bluuebunny/crossref_metadata_2025_split huggingface dataset:
Theorizing Chemical Rhetoric: Toward an Articulation of Chemistry as a Public Vocabulary
Authors: Jensen Robin E 
Published: May 2021

AbstractChemistry has been a pivotal part of scientific discovery and human life for centuries. This essay argues that chemical terms, tropes, figures, appeals, and narratives serve as powerful rhetorical features of public discourse. From affinities and atoms to dark matter and radioactivity, chemical rhetoric fulfills a central organizing function in contemporary society and shapes how people deliberate and delineate their identities, relationships, and communities. The present research demarcates chemical rhetoric as a form of nonexpert communication, and explicates its association with chemistry’s disciplinary history, as well as with technical chemical language’s grounding in key focal concepts. More specifically, it maps out a framework for defining and theorizing chemical rhetoric through three, interconnected lenses: historical–ecological, conceptual articulation, and vernacular. The overarching goal in this essay is to create an infrastructure for investigating chemistry’s longitudinal circulation and emergence as a shared public vocabulary.
A cumulative review on the utilisation of drug information services provided in India
Authors: Sadagoban G K , Baiju Aiswarya , Sanjeev Samantha , Ayilya M , Borra Swathi Swaroopa 
Published: May 2021

AbstractObjectiveTo understand the utilisation of drug information services provided in India.Key findingsThe quantitative aspects of drug information queries were assessed. Majority of the queries were raised from the General Medicine Department (43.64%) and by clinicians (41.77%), most of them were regarding adverse drug reactions (18.17%) and the prime purpose of the enquirer was to update knowledge (46.73%). The three steps for qualitative assessment and evaluation of drug information services were assessed and the overall response from the receiver’s perspective was found to be very good/satisfactory and the provider’s perspective was rendered excellent.SummaryThere is an increased need to expand the scope of drug information services and promote awareness regarding the services and it is recommended that every hospital have a Drug Information Centre.
Chk1 suppression leads to a reduction in the enhanced radiation-induced invasive capability on breast cancer cells
Authors: Adachi Takanori , Zhao Wantong , Minami Kazumasa , Yokoyama Yuhki , Okuzaki Daisuke , Kondo Rika , Takahashi Yutaka , Tamari Keisuke , Seo Yuji , Isohashi Fumiaki , Yamamoto Hirofumi , Koizumi Masahiko , Ogawa Kazuhiko 
Published: May 2021

Abstract
Radiation therapy is generally effective for treating breast cancers. However, approximately 30% of patients with breast cancer experience occasional post-treatment local and distant metastasis. Low-dose (0.5 Gy) irradiation is a risk factor that promotes the invasiveness of breast cancers. Although an inhibitor of checkpoint kinase 1 (Chk1) suppresses the growth and motility of breast cancer cell lines, no study has investigated the effects of the combined use of a Chk1 inhibitor and radiation on cancer metastasis. Here, we addressed this question by treating the human breast cancer cell line MDA-MB-231 (in vitro) and mouse mammary tumor cell line 4 T1 (in vitro and in vivo) with γ-irradiation and the Chk1 inhibitor PD407824. Low-dose γ-irradiation promoted invasiveness, which was suppressed by PD407824. Comprehensive gene expression analysis revealed that low-dose γ-irradiation upregulated the mRNA and protein levels of S100A4, the both of which were downregulated by PD407824. We conclude that PD407824 suppresses the expression of S100A4. As the result, γ-irradiation-induced cell invasiveness were inhibited.
Endoscopic management of a nail-magnet aggregation impacted in the terminal ileum: a case report
Authors: Benesch Matthew G K , Howard Carrie L , Smith Chris G 
Published: May 2021

Abstract
Management of inedible foreign objects within the gastrointestinal tract requires diligent observation and timely intervention in situations of impaction, obstruction or perforation. Here, we describe an adult patient with borderline personality disorder presenting with sequential ingestions of nails and magnets that had already passed beyond the reach of upper endoscopy. These objects conglomerated as a single mass in the terminal ileum and failed to clear into the colon despite a prolonged trial of conservative management. The patient underwent a successful colonoscopy to remove a significant component of the bezoar, allowing the remaining objects to pass through the ileocecal valve and be eliminated. This case highlights the benefits of careful conservative management with prudent use of endoscopy to manage impaction of foreign bodies in the terminal ileum in an otherwise stable adult patient.
Arabidopsis OXS3 family proteins repress ABA signaling through interactions with AFP1 in the regulation ofABI4expression
Authors: Xiao Shimin , Jiang Li , Wang Changhu , Ow David W 
Published: May 2021

AbstractAbscisic acid (ABA) and the AP2/ERF (APETALA2/ETHYLENE-RESPONSIVE FACTOR)-type transcription factor called ABA INSENSITIVE 4 (ABI4) play pivotal roles in plant growth responses to environmental stress. An analysis of seedling development in Arabidopsis ABA hypersensitive mutants suggested that OXS3 (OXIDATIVE STRESS 3), OXS3b, O3L3 (OXS3 LIKE 3), O3L4, and O3L6 were negative regulators of ABI4 expression. We therefore characterized the roles of the OXS3 family members in ABA signaling. All the above five OXS3 proteins were found to interact with AFP1 (ABI FIVE BINDING PROTEIN 1) in yeast two hybrid assays. Seven OXS3 family members including two other members O3L1 and O3L5 were found to interact with histone H2A.X, although OXS3b, O3L3, and O3L5 showed weaker interactions. ChIP-qPCR analysis showed that the absence of some of these OXS3 family proteins was associated with increased occupancy of histone γ-H2A.X at the ABI4 promoter, which also corresponded with de-repression of ABI4 expression. Repression of ABI4 expression, however, required both AFP1 and OXS3, OXS3b or O3L6. We conclude that in the absence of stress, OXS3 family proteins regulate γ-H2A.X deposition at the ABI4 promoter and that together with AFP1, OXS3 family proteins function to prevent ABA-induced growth arrest by co-repressing ABI4 through decreased promoter occupancy of histone γ-H2A.X.
Hydrogen sulfide signaling in plant adaptations to adverse conditions: molecular mechanisms
Authors: Aroca Angeles , Zhang Jing , Xie Yanjie , Romero Luis C , Gotor Cecilia 
Published: May 2021

AbstractHydrogen sulfide (H2S) is a signaling molecule that regulates critical processes and allows plants to adapt to adverse conditions. The molecular mechanism underlying H2S action relies on its chemical reactivity, and the most-well characterized mechanism is persulfidation, which involves the modification of protein thiol groups, resulting in the formation of persulfide groups. This modification causes a change of protein function, altering catalytic activity or intracellular location and inducing important physiological effects. H2S cannot react directly with thiols but instead can react with oxidized cysteine residues; therefore, H2O2 signaling through sulfenylation is required for persulfidation. A comparative study performed in this review reveals 82% identity between sulfenylome and persulfidome. With regard to abscisic acid (ABA) signaling, widespread evidence shows an interconnection between H2S and ABA in the plant response to environmental stress. Proteomic analyses have revealed persulfidation of several proteins involved in the ABA signaling network and have shown that persulfidation is triggered in response to ABA. In guard cells, a complex interaction of H2S and ABA signaling has also been described, and the persulfidation of specific signaling components seems to be the underlying mechanism.
Water and deuterium-to-hydrogen ratio in comets
Authors: Fulle Marco 
Published: May 2021

ABSTRACTRecent models find that nuclei of comets are a mixture of water-rich and water-poor cm-sized pebbles. We aim to fit the correlation of the deuterium-to-hydrogen ratio, D/H, with the nucleus active area fraction. Pebble parameters, constrained by the Rosetta mission data, depend on where pebbles accreted in the protoplanetary disc. The diversity of comets depends on the fraction of water-rich versus water-poor pebbles in each nucleus. Our model explains why the D/H values correlate to the nucleus active area fraction, and also implies that: (i) the seasonal colour cycle of the nucleus is opposite to the dust coma one; (ii) the perihelion dust size distribution is steeper than the average; (iii) water–ice content is anticorrelated with the amount of supervolatiles and with the D/H ratio. (iv) the fallout is composed of supervolatiles-depleted water-poor pebbles, whose D/H ratio was measured by the Rosetta mission; (v) the D/H average in nuclei may be different than that measured in comae and cannot be retrieved by local sample-return missions; (vi) mixing of pebbles in the outer protoplanetary disc excludes any correlation of the water content, of the D/H ratio and of the nucleus size with the disc location where comets were born.
VirusViz: comparative analysis and effective visualization of viral nucleotide and amino acid variants
Authors: Bernasconi Anna , Gulino Andrea , Alfonsi Tommaso , Canakoglu Arif , Pinoli Pietro , Sandionigi Anna , Ceri Stefano 
Published: May 2021

AbstractVariant visualization plays an important role in supporting the viral evolution analysis, extremely valuable during the COVID-19 pandemic. VirusViz is a web-based application for comparing variants of selected viral populations and their sub-populations; it is primarily focused on SARS-CoV-2 variants, although the tool also supports other viral species (SARS-CoV, MERS-CoV, Dengue, Ebola). As input, VirusViz imports results of queries extracting variants and metadata from the large database ViruSurf, which integrates information about most SARS-CoV-2 sequences publicly deposited worldwide. Moreover, VirusViz accepts sequences of new viral populations as multi-FASTA files plus corresponding metadata in CSV format; a bioinformatic pipeline builds a suitable input for VirusViz by extracting the nucleotide and amino acid variants. Pages of VirusViz provide metadata summarization, variant descriptions, and variant visualization with rich options for zooming, highlighting variants or regions of interest, and switching from nucleotides to amino acids; sequences can be grouped, groups can be comparatively analyzed. For SARS-CoV-2, we manually collect mutations with known or predicted levels of severity/virulence, as indicated in linked research articles; such critical mutations are reported when observed in sequences. The system includes light-weight project management for downloading, resuming, and merging data analysis sessions. VirusViz is freely available at http://gmql.eu/virusviz/.
The ribosome assembly factor Nop53 has a structural role in the formation of nuclear pre-60S intermediates, affecting late maturation events
Authors: Bagatelli Felipe F M , de Luna Vitorino Francisca N , da Cunha Julia P C , Oliveira Carla C 
Published: May 2021

Abstract
Eukaryotic ribosome biogenesis is an elaborate process during which ribosomal proteins assemble with the pre-rRNA while it is being processed and folded. Hundreds of assembly factors (AF) are required and transiently recruited to assist the sequential remodeling events. One of the most intricate ones is the stepwise removal of the internal transcribed spacer 2 (ITS2), between the 5.8S and 25S rRNAs, that constitutes together with five AFs the pre-60S ‘foot’. In the transition from nucleolus to nucleoplasm, Nop53 replaces Erb1 at the basis of the foot and recruits the RNA exosome for the ITS2 cleavage and foot disassembly. Here we comprehensively analyze the impact of Nop53 recruitment on the pre-60S compositional changes. We show that depletion of Nop53, different from nop53 mutants lacking the exosome-interacting motif, not only causes retention of the unprocessed foot in late pre-60S intermediates but also affects the transition from nucleolar state E particle to subsequent nuclear stages. Additionally, we reveal that Nop53 depletion causes the impairment of late maturation events such as Yvh1 recruitment. In light of recently described pre-60S cryo-EM structures, our results provide biochemical evidence for the structural role of Nop53 rearranging and stabilizing the foot interface to assist the Nog2 particle formation.
A plasmid toolbox for controlled gene expression across the Proteobacteria
Authors: Schuster Layla A , Reisch Christopher R 
Published: May 2021

AbstractControlled gene expression is fundamental for the study of gene function and our ability to engineer bacteria. However, there is currently no easy-to-use genetics toolbox that enables controlled gene expression in a wide range of diverse species. To facilitate the development of genetics systems in a fast, easy, and standardized manner, we constructed and tested a plasmid assembly toolbox that will enable the identification of well-regulated promoters in many Proteobacteria and potentially beyond. Each plasmid is composed of four categories of genetic parts (i) the origin of replication, (ii) resistance marker, (iii) promoter-regulator and (iv) reporter. The plasmids can be efficiently assembled using ligation-independent cloning, and any gene of interest can be easily inserted in place of the reporter. We tested this toolbox in nine different Proteobacteria and identified regulated promoters with over fifty-fold induction range in eight of these bacteria. We also constructed variant libraries that enabled the identification of promoter-regulators with varied expression levels and increased inducible fold change relative to the original promoter. A selection of over 50 plasmids, which contain all of the toolbox's genetic parts, are available for community use and will enable easy construction and testing of genetics systems in both model and non-model bacteria.
DIOXYGENASE FOR AUXIN OXIDATION 1 catalyzes the oxidation of IAA amino acid conjugates
Authors: Müller Karel , Dobrev Petre Ivanov , Pěnčík Aleš , Hošek Petr , Vondráková Zuzana , Filepová Roberta , Malínská Kateřina , Brunoni Federica , Helusová Lenka , Moravec Tomáš , Retzer Katarzyna , Harant Karel , Novák Ondřej , Hoyerová Klára , Petrášek Jan 
Published: May 2021

Abstract
Together with auxin transport, auxin metabolism is a key determinant of auxin signaling output by plant cells. Enzymatic machinery involved in auxin metabolism is subject to regulation based on numerous inputs, including the concentration of auxin itself. Therefore, experiments characterizing altered auxin availability and subsequent changes in auxin metabolism could elucidate the function and regulatory role of individual elements in the auxin metabolic machinery. Here, we studied auxin metabolism in auxin-dependent tobacco BY-2 cells. We revealed that the concentration of N-(2-oxindole-3-acetyl)-l-aspartic acid (oxIAA-Asp), the most abundant auxin metabolite produced in the control culture, dramatically decreased in auxin-starved BY-2 cells. Analysis of the transcriptome and proteome in auxin-starved cells uncovered significant downregulation of all tobacco (Nicotiana tabacum) homologs of Arabidopsis (Arabidopsis thaliana) DIOXYGENASE FOR AUXIN OXIDATION 1 (DAO1), at both transcript and protein levels. Auxin metabolism profiling in BY-2 mutants carrying either siRNA-silenced or CRISPR-Cas9-mutated NtDAO1, as well as in dao1-1 Arabidopsis plants, showed not only the expected lower levels of oxIAA, but also significantly lower abundance of oxIAA-Asp. Finally, ability of DAO1 to oxidize IAA-Asp was confirmed by an enzyme assay in AtDAO1-producing bacterial culture. Our results thus represent direct evidence of DAO1 activity on IAA amino acid conjugates.
Effect of jet lag on brain white matter functional connectivity
Authors: Zhang Feifei , Yang Zhipeng , Qin Kun , Sweeney John A , Roberts Neil , Jia Zhiyun , Gong Qiyong 
Published: May 2021

AbstractBackgroundA long-haul flight across more than five time zones may produce a circadian rhythm sleep disorder known as jet lag. Little is known about the effect of jet lag on white matter (WM) functional connectivity (FC).ObjectiveThe present study is to investigate changes in WM FC in subjects due to recovery from jet lag after flying across six time zones.MethodsHere, resting-state functional magnetic resonance imaging was performed in 23 participants within 24 hours of flying and again 50 days later. Gray matter (GM) and WM networks were identified by k-means clustering. WM FC and functional covariance connectivity (FCC) were analyzed. Next, a sliding window method was used to establish dynamic WM FC. WM static and dynamic FC and FCC were compared between when participants had initially completed their journey and 50 days later. Emotion was assessed using the Positive and Negative Affect Schedule and the State Anxiety Inventory.ResultsAll participants were confirmed to have jet lag symptoms by the Columbian Jet Lag Scale. The static FC strengthes of cingulate network (WM7)- sensorimotor network and ventral frontal network- visual network were lower after the long-haul flight compared with recovery. Corresponding results were obtained for the dynamic FC analysis. The analysis of FCC revealed weakened connections between the WM7 and several other brain networks, especially the precentral/postcentral network. Moreover, a negative correlation was found between emotion scores and the FC between the WM7 and sensorimotor related regions.ConclusionsThe results of this study provide further evidence for the existence of WM networks and show that jet lag is associated with alterations in static and dynamic WM FC and FCC, especially in sensorimotor networks. Jet lag is a complex problem that not only is related to sleep rhythm but also influences emotion.
High frequency ultrasonography: reliable tool to measure skin fibrosis in SSC? A systematic literature review and additional pilot study
Authors: Vanhaecke Amber , Cutolo Maurizio , Heeman Lise , Vilela Veronica , Deschepper Ellen , Melsens Karin , Smith Vanessa 
Published: May 2021

Abstract

Objectives
To investigate the reliability of high frequency ultrasound (HFUS) in measuring skin fibrosis in SSc.

Methods
First, a systematic review (according to PRISMA) was conducted to identify studies that documented HFUS’ reliability in SSc as a primary outcome. Then, in an additional pilot study, the inter- and intra-rater reliability of two investigators performing HFUS for dermal thickness (DT) measurements in a standardized manner across all 17 areas of the modified Rodnan Skin Score was evaluated in a group of 59 SSc patients and descriptively in 44 healthy controls (HC). As an external validation, DT measurements by HFUS were performed in a separate group of 30 SSc patients by the same first and another third investigator.

Results
The systematic review retained few (4/1719 unique records) small-scale studies, with mixed study populations (combining SSc and HC). The reported data herein are suggestive of the inter-/intra-rater reliability of HFUS (intra-class correlation coefficient [ICCs] ranging 0.65–0.94/0.55–0.96, respectively). Additionally, in our pilot study, inter-/intra-rater reliability was good-to-excellent in both SSc groups and HC (ICCs ranging 0.70–0.97/0.70–0.98 and 0.65–0.95/0.63–0.96, respectively).

Conclusion
The identified small-scale studies were not only combining data from SSc and HC, they were also heterogeneous in terms of technical aspects (probes and frequency), image acquisition methods ([number of] areas assessed) and definitions used for skin thickness, which prevents drawing unequivocal conclusions. Despite these limitations, our standardized pilot study corroborated the findings in literature, paving the way for the applicability of HFUS as a reliable (complementary) tool to quantify skin fibrosis in SSc.
Virtual property: trusts of cryptocurrencies and other digital assets
Authors: Cooper Gilead 
Published: May 2021

Abstract
The question whether digital assets such as cryptocurrencies are “property” as a matter of law has been the subject of academic debate almost since the inception of the bitcoin network in 2009. The question arises from the fact that such assets, and cryptocurrencies in particular, do not fit into traditional categories of property as understood by the common law, being neither “choses in possession” nor “choses in action”. However, following a series of decisions in the last couple of years, it now seems reasonably settled that this question will be answered in the affirmative, although no cases have so far reached the highest level of the courts. The consequences have yet to be worked out. One obvious consequence is that cryptocurrencies can be the subject of a trust. However, the unique features of digital assets give rise to a number of interesting problems, both theoretical and practical, on which there is little authoritative guidance.
Molecular phylogeny of European Runcinida (Gastropoda, Heterobranchia): the discover of an unexpected pool of complex species, with special reference to the case ofRuncina coronata
Authors: Araujo Ana Karla , Pola Marta , Malaquias Manuel Antonio E , Ballesteros Manuel , Vitale Fabio , Cervera Juan Lucas 
Published: May 2021

AbstractRuncinida is a small heterobranch order of sea slugs with 61 known species distributed worldwide across temperate and tropical latitudes with two established families (Ilbiidae and Runcinidae). Little is known about the phylogenetic relationships within Runcinida. Here, we present the first molecular phylogeny of the order with an emphasis on European species and we discuss the taxonomic status of the type species Runcina coronata. Molecular phylogenetics based on the mitochondrial genes cytochrome c oxidase subunit I and 16S rRNA and nuclear gene histone H3 is used to investigate relationships between species. Detailed morpho-anatomical worked was additionally employed to study Runcina coronata. Our results suggest the monophyly of Runcinida and showed that Runcina coronata is a complex of four species, namely: R. coronata proper, R. aurata and two new species here formally described (R. caletensis sp. nov. and R. tingensis sp. nov.).