bluuebunny/crossref_metadata_2025_split huggingface dataset:
WITHDRAWN: Effects of GABAA Receptors in Dorsal Hippocampus on Memory Retention of Cholestatic Rats
Authors: Chen Ruili , Zhang Dongdong , Tayyebi Sepideh , Li Nini 
Published: November 2021

Abstract
The molecular mechanisms that result in cognitive deficits following cholestasis are mainly unknown. As there are many GABAA receptors in the hippocampus CA1 region and the crucial role for GABA in modulating memory, we evaluated the effects of GABAA receptor agents in the CA1 of cholestatic rats on memory retention. The interaction between GABAergic and opioidergic systems in the CA1 on memory was also investigated. The effects of administration of GABAA receptor agonist and antagonist, muscimol (60, 120 and 240 ng/ side) and bicuculline (100, 200 and 400 ng/ side), into CA1 on memory retention were studies using passive avoidance learning (PAL) task in bile duct ligated (BDL) rats. Naloxone (250 ng/ side), the mu-opioid receptor antagonist, was also co-administered alone or with bicuculline (400 ng/ side) to indicate the interaction between opioidergic and GABAergic system. Cholestasis inhibited memory retrieval is shown by the decrease in the step-through latency (STLr). Administration of muscimol or bicuculline alone after training potentiated or attenuated respectively amnesia in BDL rats dose-dependently. Naloxone (250 ng /side) alone increased STLr in BDL-treated rats. Bicuculline (100 ng/side) alone antagonized the amnesic effect of muscimol (120 ng/side). Co-administration of bicuculline and naloxone or muscimol and naloxone caused a significant difference in STLr compared to only naloxone treated rats which show the interaction between two systems on memory retention in cholestssis. Bicuculline (100 ng/side) microinjection alone antagonized the amnesic effect of muscimol (120 ng/side). We indicated the contribution of intra-CA1 GABAA receptors on memory retention in cholestatic rats by the PAL test. Blockade of each GABAA or mu-opioid receptors alone could attenuate the amnesia in BDL rats. Furthermore, blockade of both GABAA or mu-opioid receptors reversed the memory deficit in BDL-treated rats, which shows the interaction between GABAergic and opioidergic systems on memory retention in this test.
Production Enhancement of Human Adipose-Derived Mesenchymal Stem Cells By Low-Intensity Ultrasound Stimulation
Authors: Min Soohong , Byeon Yungsun , Kim Min , Lee Youngjun , Lee Hongki , Paeng Dong-Guk 
Published: November 2021

Abstract
Background: Low-intensity ultrasound (LIUS) has been used to increase the proliferation rate of various stem cells including human adipose-derived mesenchymal stem cells (hAdMSCs). hAdMSCs is now commercially produced for various therapeutic applications. The purpose of this study was to show feasibility of enhancing the productivity of cell culture during 16-day cell culturing and increasing proliferation rate of hAdMSCs by LIUS stimulation with appropriate ultrasound parameters. Methods: Beam patterns of 5 and 10 MHz ultrasound transducers were measured to confirm the area of stimulation. The intensity of sound waves transmitted through a Petri-dish was measured in situ for quantitative evaluation. Bromodeoxyuridine (BrdU) incorporation assay was performed to search for appropriate parameters for LIUS stimulation of hAdMSCs. Cell culture medium supplemented with 8% fetal bovine serum (FBS) in a 35 mm Petri-dish was used for 16 days with subculture from 2 to 6 passage. Results: A frequency of 5 MHz, an intensity of 300 , a duration of 10 minutes per day, and continuous waves with 100% duty cycle were the best parameters according to the BrdU assay of proliferation rate of hAdMSCs. LIUS stimulation group had about 3.25-fold greater number of cells from passage 2 to 6 compared with the control group. Doubling time was decreased to 4.44 hours in average. Cell viability was the same between the control and LIUS stimulation groups.Conclusions: This study of enchanced proliferation rate and cell culture productivity of hAdMSCs by LIUS stimulation may lay the foundation for the application of LIUS stimulation in cell therapeutic industry by reducing the production cost and time required for cell therapy.
Synthesis, Characterization And Antimicrobial Activity of Copper-Metal Organic Framework (Cu-MOF) And Its Modification By Melamine
Authors: Abdelmoaty A.S. , El-Beih A.A. , Hanna A.A. 
Published: November 2021

Abstract
Copper- Metal organic framework (Cu-MOF) and melamine/ Cu-MOF (MCu-MOF) samples were prepared by the hydrothermal process. The produced powder was dried and characterized by using the FTIR, X-ray techniques. The thermal gravimetry (TG) analysis was performed to detect the thermal stability of the product. On other hand, the morphology and the surface area of the powder were carried. The bioactivity of the powder was carried by measuring the inhibition zone diameter around samples in (mm). The results of characterization showed that the formation of Cu-MO and melamine/ Cu-MOF in crystalline state in both samples, the surface area of Cu-MOF is equal to 1350 m2 g-1 while increase to 1410 m2 g-1 by incorporate by melamine into the Cu-MOF. The thermal behavior (TG) of the Cu-MOF showed three sequence stages attributed to the moisture evaporation, degradation of the Cu-MOF and forming the Cu-O as end product respectively. For melamine incorporated to Cu-MOF (MCu-MOF), the TG profile shows that the split of the main degradation into two parts resulting from the presence of melamine. The bioactivities of the both samples were tested against microbial strains. The results showed that the Cu-MOF and MCu-MOF have insignificant antimicrobial activity against gram positive of bacteria and Fungi. While for gram negative of bacteria it is observed a considerable effect.
Parent-of-origin effects in the UK Biobank
Authors: Delaneau Olivier , Hofmeister Robin , Rubinacci Simone , Ribeiro Diogo , Kutalik Zoltan , Buil Alfonso 
Published: November 2021

Abstract
Identical genetic variations can have different phenotypic effects depending on their parent of origin (PofO). Yet, studies focussing on PofO effects have been largely limited in terms of sample size due to the need of parental genomes or known genealogies. Here, we used a novel probabilistic approach to infer PofO of individual alleles in the UK Biobank that does not require parental genomes nor prior knowledge of genealogy. Our model uses Identity-By-Descent (IBD) sharing with second- and third-degree relatives to assign alleles to parental groups and leverages chromosome X data in males to distinguish maternal from paternal groups. When combined with robust haplotype inference and haploid imputation, this allowed us to infer the PofO at 5.4 million variants genome-wide for 26,393 UK Biobank individuals. We used this large dataset to systematically screen 59 biomarkers and 38 anthropomorphic phenotypes for PofO effects and discovered 101 significant associations, demonstrating that this type of effects is widespread. Notably, we retrieved well known PofO effects, such as the MEG3/DLK1 locus on platelet count, and we discovered many new ones often at loci outside currently known imprinted regions and previously thought to harbour additive associations, implying that the underlying molecular mechanisms may be more complex than expected.
Factors Influencing Survival Time in Patients Treated for Oesophageal Cancer with Palliative Intent in South Africa
Authors: Ferndale Lucien , Ayeni Oluwatosin A , Chen Wenlong Carl , Aldous Colleen 
Published: November 2021

Abstract
BackgroundMost patients presenting with oesophageal cancer to South African institutions present late with advanced disease and are eligible for palliative treatment only. The optimal management of these patients is dependent on their expected survival but factors affecting survival in our setting is unknown. MethodsWe assessed factors influencing survival in patients diagnosed with oesophageal squamous cell carcinoma at Grey's hospital in KwaZulu-Natal, South Africa. Factors assessed for effect on prognosis included patient demographics, clinical and laboratory data and tumour factors. Patients were divided into two groups; those that survived for less than or equal to three months and those that survived for more than three months.Univariate and multivariate analyses were performed to determine which factors significantly influenced whether patients survived for more than three months or not. ResultsA total of 384 patients were entered into the study. There were 218 males with a female to male ratio of 1:1.3. The median survival of the cohort was 3.7 months. One hundred and sixty-one (41.9%) patients survived three months or less, while 223 (58.1%) of patients survived more than three months. Factors that influenced survival on univariate and multivariate analysis were the area of residence, body mass index, serum albumin and performance status.ConclusionArea of residence, body mass index, serum albumin and performance status significantly affect survival in patients treated palliatively for advanced oesophageal cancer at our institution. This knowledge can be used to plan the optimal management algorithm for these patients.
The differential efficacy of systemic therapy: a protocol for a systematic review and meta-analysis
Authors: Braus Niels , von Oepen Rebecca , Immel Nina , Wichmann Johanna , Frankman Christian , Messner Eva-Maria , Zemp Martina , Hunger-Schoppe Christina 
Published: November 2021

Abstract
Background: Systemic therapy is a widespread evidence-based psychotherapy approach. Its main goal revolves around the concept of viewing mental symptoms within the context of social systems (e.g., families, couples). Systematic reviews and meta-analyses have confirmed that systemic therapy significantly reduces symptom severity of DSM- or ICD-diagnosed patients. This meta-analysis aims to investigate the differential efficacy of systemic therapy, taking into account moderators (e.g., allegiance, adherence) and outcomes (system functioning) not considered in previous meta-analytical investigations.Methods: To conduct a comprehensive literature search, we will optimize search strategies from previous systematic reviews on systemic therapy. The search strategy presented in this protocol has improved the precision and sensitivity by using an iterative validation and optimization process. We will conduct the literature search in multiple electronic databases (PsycInfo, Pubmed, Embase, and Cochrane Central Register of Controlled Trials (CENTRAL)). We will include all randomized controlled trials that report quantitative outcomes for symptom change and/or system functioning. For the calculations, we will conduct a Bayesian meta-analysis for the outcomes based on a random-effects model, a Bayesian meta-regression for the moderators, and Bayesian subgroup analysis for disorder-specific differences.Discussion: Understanding the differential efficacy of systemic therapy is essential for the conceptualization, performance, and analysis of future research and therapeutic practice. This meta-analysis faces potential limitations associated with the definition of systemic therapy, as well as methodological problems in systemic therapy research.
A Comparison Survey of Paramedic Level of Confidence in Handling Paediatric Versus Adult Patients- Riyadh City
Authors: Alharthy Nesrin , Alfraih Ethar , Alenazi Atheer , Alaiban Reem , Alanazi Rahaf , Alsuwais Sara , Almutairi Mohammad 
Published: November 2021

Abstract
Background:Emergency Medical Services (EMS) is a national network of prehospital services coordinated to provide aid and medical assistance to pediatric and adult patients. Studies indicate that a paramedic’s level of confidence in handling an adult patient is higher than that in handling a paediatric patient. The study objective is to explore paramedics’ levels of confidence in handling pediatric patients as compared to that in handling adult patients in Riyadh, Saudi Arabia. Method:A cross-sectional study targeted paramedics in different institutions in the city of Riyadh after , a self-administered questionnaire were used to measure level of confidence in handling adult compared to pediatric in different given clinical scenarios using five point Likert scaleResults:A total of 144 participants completed the survey, with a response rate of 90%. Of these, 91.7% were male, 42.4% worked in pre-hospital care, and 43.8% worked in-hospital station. The sample was comprised of both paramedics who obtained their qualifications in the Kingdom of Saudi Arabia (KSA) (53.5%) and those who obtained their qualifications outside the KSA (46.5%). Across the sample, 97% were confident in handling adults, while 78.5% were confident in handling paediatric patients. The paramedics’ levels of confidence in handling adult and paediatric patients were compared in terms of ten specific conditions and skills using a five-point Likert scale, where a set score based on response of 10 was the minimum and 50 was the maximum. The difference in confidence level with paediatric (median = 36, IQR: 29,39) and adult (Median= 47, IQR: 42,50) patients was statistically significant, with a p-value of 0.001. Conclusions: The findings of this study are consistent with international studies showing that paramedics are more confident in handling adult patients than pediatric patients. This study recommends the dissemination of its findings to stakeholders for advanced training in paediatric.
One-Pot Synthesis of Quinazoline Derivatives Using Copper Nanocatalyst Stabilized on Graphene Oxide Functionalized by N1,N2-bis((Pyridine-2-yl)Methyl)Benzene-1,2-Diamine
Authors: Emami-Nori Alahyar , Karamshahi Zahra , Ghorbani-Vaghei Ramin 
Published: November 2021

Abstract
Having a biological property is just one of features of N-heterocycles. Another thing is to be dramatically well-designed. In other words, awe-inspiring combination of materials and the thing that really makes these materials just unique of its kind is nothing but its medical properties. That is why, majority of scientists are full of enthusiasm for synthesizing of these types of materials. ln this article, we have reported five effective methods for synthesizing quinazoline, which is a immensely important group of N-heterocycles with using copper nanocatalyst stabilized on graphene oxide functionalized by N1,N2-bis((pyridine-2-yl)methyl)benzene-1,2-diamine. Moreover, we have investigated the effects of substitutions connected to aldehydes, benzylamines and benzamides as a carbon source of quinazoline heterocyclic ring. Finally, we have assessed effects kinds of ammonium salts as a nitrogen source of quinazoline heterocyclic ring.
Galactooligosaccharides and Xylo-oligosaccharides Altering the Caecal Microbiome, Metabolome, and Transcriptome of Chickens Revealed by a Multi-Omics Analysis
Authors: Yang Chaowu , Qiu Mohan , Yu Chunlin , Peng Han , Song Xiaoyan , Yang Li , Xiong Xia , Hu Chenming , Zhang Zengrong , Chen Jialei , Xia Bo , Du Huarui , Li Qingyun , Jiang Xiaosong 
Published: November 2021

Abstract
Backgroud Studies have shown that prebiotics could affect meat quality, but the underlying mechanism are poorly understood. This study aimed to investigate whether prebiotics affect chicken’s meat quality through gut microbiome and metabolome. Methods The gut content were collected from chickens fed with or without prebiotics (galactooligosaccharides or xylo-oligosaccharides) and subjected to microbiome and metabolome analyses, and chicken breast was performed transcriptome sequencing. Results The prebiotics altered proportions of microbiota in gut contents at different levels, especially microbiota in the phylum of Bacteroidetes and Firmicutes, such as genus of Alistipes, Bacteroides, and Faecalibacterium. The prebiotics also altered contents of caecal metabolites such as lysophosphatidylcholine (lysoPC), intramuscular fat and flavor compound (Benzaldehyde and myristic acid). Differentially expressed genes (DEGs) induced by prebiotics were significantly involved in regulation of lipolysis inadipocytes and adipocytokine signaling pathway. Changes in gut microbiota and metabolites were remarkably correlated such as Bacteroidetes and Firmicutes was respectively positively and negatively correlated with lysoPC. DEGs were also interacted with caecal metabolites. Conclusion These findings integrated and incorporated link among gut microbiota, metabolites and transcriptome, which proposed prebiotics may affect meat quality and flavor of chickens.
The Development and Concurrent Validity of a Multi-sensor-based Frailty Toolkit for In-home Frailty Assessment
Authors: Bian Chao , Ye Bing , Mihailidis Alex 
Published: November 2021

Abstract

BackgroundEarly identification of frailty is crucial to prevent or reverse the syndrome but faces challenges due to frailty’s insidious onset. Monitoring behaviroal changes in real life may offer opportunities for early identifying frailty. Home-based technologies for assessing frailty have shown potentials. However, they are still in early stage of development with many types of technologies and behavioural and physical signs associated with frailty underexamined. This study presented a sensor-based system that used heterogeneous sensors and cloud technologies to monitor behavioural and physical signs of frailty from home settings. We aimed to validate the concurrent validity of the sensor system.MethodsThe sensor system consisted of motion, door, distance and mat sensors, a smart speaker, and a smart weight scale. The selection of these sensors was based on behavioural and physical signs found significantly associated with frailty. These signs included room-level physical activity, life space, sedentary behaviour, stair climbing, self-report exhaustion, and weight. Older adults’ voices were included in the system design. The sensor system prototype was tested in a simulated home lab environment with nine young, healthy participants. Cohen’s kappa and Bland-Altman Plot statistical methods evaluated the agreements between the sensor and ground truth measurements.ResultsExcellent concurrent validity was achieved for the smart speaker (100% agreement), the motion sensor (a kappa value of k=0.938), the door sensor (87.5% agreement, 100% if excluding outliers), the mat sensor (mean difference of -0.286, 95% limits of agreement) and the distance sensor (mean difference of 0.526, 95% limits of agreement). The smart weight scale and traditional weight scale had a significant difference in weight measurements. However, bivariate correlation showed a strong, positive correlation between the two measurements (r=0.942, n=24, p<0.001). ConclusionsOverall, FT is reliable for monitoring physical and behavioural signs of frailty in home settings. The ambient sensors and the smart speaker in FT showed excellent concurrent validity, while the smart weight scale needs further validation. This work laid the ground for the next step of the research to test the toolkit with frail and non-frail older adults to validate the system’s clinical effectiveness.
Regional Agricultural Drought Risk Assessment Method Based on Risk Transforming Process
Authors: WEI Yanqi , CUI Yi , JIN Juliang , ISHIDAIRA Hiroshi , LI Haichao , JIANG Shangming , ZHO Rongxing , ZHOU Liangguang 
Published: November 2021

Abstract
Drought risk management can effectively reduce drought losses and improve drought resistance capability, of which drought risk assessment is the core issue. This study evaluated the agricultural drought risk in Huaibei Plain of Anhui Province in China by the approach of constructing drought loss risk curves and risk distribution maps. The results showed that: 1) The drought events that occurred in northern regions (Huaibei and Suzhou) were with the characteristics of high-frequency and low-intensity, while in southern regions (Huainan and Bengbu), the occurring characteristics were low-frequency, high-intensity, and long-duration. 2) Without irrigation, Fuyang was the high-risk region with more than 80% potential yield loss rate, while Huainan was the relatively low-risk area with a potential yield loss of 50%. 3) Irrigation had a significant effect on reducing drought risk loss, while the efficiency was influenced by the spatio-temporal distribution of precipitation. The irrigation scheme in study area still remains to be optimized based on the characteristics of precipitation and crop growth. This study established and practiced a quantitative framework for regional drought risk assessment by creating drought risk curves and risk maps, which have significant value in improving the regional agricultural drought risk management level.
The Use of Intraoperative Radiation Therapy (IORT) in Multimodality Management of Cancer Patients. A Single Institution Experience.
Authors: Elashwah Ahmed , Alsuhaibani Abdullah , Azzam Ayman , Moftah Belal , Hussein Muhammed , ALramahi Shada , Amin Tarek , Mahmoud Rana , BREAKEIT Mohammed , Hassan Zeinab , Alzahrani Ali 
Published: November 2021

Abstract
Background Intraoperative radiation therapy (IORT) is a highly conformal technique given in the operating room in many cancer sites for better tumor local control by increasing the tumor radiation dose without exceeding normal tissues tolerance doses.Purpose Assess the feasibility of Intra operative radiation therapy (IORT) and short-term toxicity in patients with different cancer sites treated with a multidisciplinary protocols including IORT.Patients & MethodsMedical records of cancer patients who received IORT at King Faisal Specialized Hospital and Research center(KFSHRC), Riyadh, Saudi Arabia from 2013 until 2017 were retrospectively reviewed.ResultsTotal 188 patients with 212 IORT applications were analyzed. Twenty-four patients had more than one application.116 patients were males. Median age at time of diagnosis was 49.5years (19-77). Hundred thirty four patients had primary while 54 cases had recurrent disease. Gastro esophageal cancer and soft tissue sarcoma were the most frequent diagnosis in 49 patients followed by colorectal cancer in 35 patients. Major surgeries with curative intent done in 183 patients (97.3%) and 118 patients (62.8%) had hyperthermic intraperitoneal chemotherapy (HIPEC) in addition to IORT. The 30days postoperative mortality rate was 3.2%. Fifty-three (28.2%) patients develop Grade III-IV complications according to Clavien-Dindo grading system. ConclusionThe data presented discussing treatment modalities for different malignant tumors which are treated and may benefit from using IORT technique as a part of multimodality treatment. IORT seems safe and feasible, however a longer follow-up is needed for proper evaluation and defining the role of IORT in tailored multimodality approach.