bluuebunny/crossref_metadata_2025_split huggingface dataset:
Analysis of the transcriptome of bovine endometrial cells isolated by laser micro-dissection (2): impacts of post-partum negative energy balance on stromal, glandular and luminal epithelial cells
Authors: Chankeaw Wiruntita , Lignier Sandra , Richard Christophe , Ntallaris Theodoros , Raliou Mariam , Guo Yongzhi , Plassard Damien , Bevilacqua Claudia , Sandra Olivier , Andersson Göran , Humblot Patrice , Charpigny Gilles 
Published: June 2021

Abstract
Background
In post-partum dairy cows, the energy needs to satisfy high milk production induces a status of more or less pronounced Negative Energy Balance (NEB). NEB associated with fat mobilization impairs reproductive function. In a companion paper, we described constitutive gene expression in the three main endometrial cell types (stromal, glandular and luminal epithelial cells) isolated by laser capture micro-dissection (LCM) showing the specificities of their transcriptomic profiles. This study investigates the specific impact of NEB on gene expression in these cells around 80 days after parturition at day 15 of the oestrus cycle and describes their specific response to NEB.

Results
Following the description of their constitutive expression, the transcriptome profiles obtained by RNA sequencing of the three cells types revealed that differences related to the severity of NEB altered mainly specific patterns of expression related to individual cell types. Number of differentially expressed genes between severe NEB (SNEB) and mild NEB (MNEB) cows was higher in ST than in LE and GE, respectively. SNEB was associated with differential expression of genes coding for proteins involved in metabolic processes and embryo-maternal interactions in ST. Under-expression of genes encoding proteins with functions related to cell structure was found in GE whereas genes encoding proteins participating in pro-inflammatory pathways were over-expressed. Genes associated to adaptive immunity were under-expressed in LE.

Conclusion
The severity of NEB after calving is associated with changes in gene expression around 80 days after parturition corresponding to the time of breeding. Specific alterations in GEs are associated with activation of pro-inflammatory mechanisms. Concomitantly, changes in the expression of genes encoding proteins involved in cell interactions and maternal recognition of pregnancy takes place in ST. The combination of these effects possibly altering the uterine environment and embryo maternal interactions may negatively influence the establishment of pregnancy.
Ecological strategies of biological and chemical control agents on wildfire disease of tobacco (Nicotiana tabacum L.)
Authors: Liu Tianbo , Gu Yabing , Zhou Zhicheng , Liu Zhenghua , Yin Huaqun , Qin Chong , Yi Tuyong , Tao Jiemeng 
Published: June 2021

Abstract
Background
To investigate the ecological effects of chemical and biological control methods on tobacco wildfire disease, a plot field experiment was conducted to compare the control efficiency and mechanisms of a chemical pesticide (kasugamycin wettable powder, KWP) and a biological control agent (BCA) through high-throughput sequencing of bacterial 16S rRNA genes.

Results
The results showed that the BCA displayed better performance in decreasing the disease index and morbidity of tobacco than the chemical pesticide. By monitoring the endophytic community within tobacco leaves, it was found that the control effects of these two methods might be mediated by different changes in the endophytic bacterial communities and community assembly patterns. The application of either method decreased the taxonomic diversity of the leaf endophytic community. Compared to the BCA, KWP showed a more significant effect on the endophytic community structure, while the endophytic community treated with the BCA was able to return to the original state, which presented much lower disease infection. The disease control efficiency of KWP and BCA treatments might be achieved by increasing the abundance of Sphingomonas and Streptophyta, respectively. Furthermore, an analysis of the ecological processes in community assembly indicated that the BCA strengthened the homogeneous and variable selection, while KWP enhanced ecological drift.

Conclusions
The results suggested different control mechanisms between KWP and BCA treatments, which will help in developing diverse ecological strategies for plant disease control.
Resilience mediates the effect of self-efficacy on symptoms of prenatal anxiety among pregnant women: a nationwide smartphone cross-sectional study in China
Authors: Ma Ruqing , Yang Fengzhi , Zhang Lijuan , Sznajder Kristin K. , Zou Changqing , Jia Yajing , Cui Can , Zhang Weiyu , Zhang Wenzhu , Zou Ning , Yang Xiaoshi 
Published: June 2021

Abstract
Background
Prenatal anxiety is one of the most prevalent mental disorders during pregnancy. This study assessed the prevalence of prenatal anxiety and examined whether resilience could play the mediating role in the association between self-efficacy and symptoms of prenatal anxiety among pregnant women in China.

Methods
A nationwide smartphone cross-sectional study was carried out in three cities (Shenyang of Liaoning Province, Zhengzhou of Henan Province and Chongqing Municipality) in China from July 2018 to July 2019. The questionnaire consisted of questions on demographic characteristics, the Generalized Anxiety Disorder Scale (GAD-7), the Chinese version of General Self-efficacy Scale (GSES), and the 14-item Wagnild and Young Resilience Scale (RS-14). A total of 665 pregnant women were recruited in this study. A hierarchical multiple regression model was employed to explore the associate factors and mediators of symptoms of prenatal anxiety. A structural equation model was employed to test the hypothesis that resilience mediates the association between self-efficacy and symptoms of prenatal anxiety.

Results
The prevalence of symptoms of prenatal anxiety was 36.4% in this study. Self-efficacy was negatively correlated with symptoms of prenatal anxiety (r = -0.366, P < 0.01). Resilience had a significant positive correlation with self-efficacy (r = 0.612, P < 0.01) and had a negative correlation with symptoms of prenatal anxiety (r = -0.427, P < 0.01). The hierarchical multiple regression model indicated that self-efficacy and resilience were the main factors associated with symptoms of prenatal anxiety and contributed to 11.9% and 6.3% to the variance of symptoms of prenatal anxiety, respectively. Resilience served as a mediator between self-efficacy and symptoms of prenatal anxiety (a*b = -0.198, Bias-corrected and accelerated bootstrap 95% Confidence interval: -0.270, -0.126).

Conclusions
Self-efficacy was a negative predictor of symptoms of prenatal anxiety among pregnant women. Moreover, resilience mediated the relation between self-efficacy and symptoms of prenatal anxiety among pregnant women in China. It was observed in this study that psychological interventions might be beneficial for pregnant women to relieve symptoms of prenatal anxiety through improved self-efficacy and resilience.
COVID-19 infection across workplace settings in Qatar: a comparison of COVID-19 positivity rates of screened workers from March 1st until July 31st, 2020
Authors: Al-Kuwari Mohamed Ghaith , Al-Nuaimi Asma Ali , Abdulmajeed Jazeel , Semaan Sandy , Al-Romaihi Hamad Eid , Kandy Mujeeb Chettiyam , Swamy Selvakumar 
Published: June 2021

Abstract
Introduction
COVID-19 transmission was significant amongst Qatar’s working population during the March–July 2020 outbreak. The study aimed to estimate the risk of exposure for COVID-19 across various workplace settings and demographics in the State of Qatar.

Methods
A cross-sectional study was conducted utilizing surveillance data of all workplaces with 10 or more laboratory-confirmed cases of COVID-19. These workplaces were categorized using a mapping table adapted from the North American Industry Classification System (NAICS) codes, 2017 version. The data was then analyzed to estimate and compare the positivity rate as an indicator of the risk of developing COVID-19 infection across various workplace settings in the State of Qatar.

Results
The highest positivity rate was reported amongst the Construction & Related (40.0%) and the Retail & Wholesale Trade sectors (40.0%), whereas, the lowest positivity rate was attributed to the healthcare workplace setting (11.0%). The highest incidence of COVID-19 infections occurred in South Asian nationalities and in the male gender. The private funded sector employees have seen higher positivity rate than employees of the governmental funded sector.

Conclusion
The elevated risk of infection in Construction and Retail & Wholesale Trade is probably due to environmental and educational vulnerabilities. The predominant labor force of those workplace categories is South Asian craft and male manual workers. Alternatively, the better containment of the healthcare workplace setting can be attributed to the enforcement of infection control and occupational safety measures. These findings imply the importance of using preventive and surveillance strategies for high-risk workplace settings appropriately.
Dietary fiber intake, the gut microbiome, and chronic systemic inflammation in a cohort of adult men
Authors: Ma Wenjie , Nguyen Long H. , Song Mingyang , Wang Dong D. , Franzosa Eric A. , Cao Yin , Joshi Amit , Drew David A. , Mehta Raaj , Ivey Kerry L. , Strate Lisa L. , Giovannucci Edward L. , Izard Jacques , Garrett Wendy , Rimm Eric B. , Huttenhower Curtis , Chan Andrew T. 
Published: June 2021

AbstractBackgroundA higher intake of dietary fiber is associated with a decreased risk of chronic inflammatory diseases such as cardiovascular disease and inflammatory bowel disease. This may function in part due to abrogation of chronic systemic inflammation induced by factors such as dysbiotic gut communities. Data regarding the detailed influences of long-term and recent intake of differing dietary fiber sources on the human gut microbiome are lacking.MethodsIn a cohort of 307 generally healthy men, we examined gut microbiomes, profiled by shotgun metagenomic and metatranscriptomic sequencing, and long-term and recent dietary fiber intake in relation to plasma levels of C-reactive protein (CRP), an established biomarker for chronic inflammation. Data were analyzed using multivariate linear mixed models.ResultsWe found that inflammation-associated gut microbial configurations corresponded with higher CRP levels. A greater intake of dietary fiber was associated with shifts in gut microbiome composition, particularly Clostridiales, and their potential for carbohydrate utilization via polysaccharide degradation. This was particularly true for fruit fiber sources (i.e., pectin). Most striking, fiber intake was associated with significantly greater CRP reduction in individuals without substantialPrevotella copricarriage in the gut, whereas those withP. copricarriage maintained stable CRP levels regardless of fiber intake.ConclusionsOur findings offer human evidence supporting a fiber-gut microbiota interaction, as well as a potential specific mechanism by which gut-mediated systemic inflammation may be mitigated.
Microalgal-based feed: promising alternative feedstocks for livestock and poultry production
Authors: Saadaoui Imen , Rasheed Rihab , Aguilar Ana , Cherif Maroua , Al Jabri Hareb , Sayadi Sami , Manning Schonna R. 
Published: June 2021

AbstractThere is an immediate need to identify alternative sources of high-nutrient feedstocks for domestic livestock production and poultry, not only to support growing food demands but also to produce microalgae-source functional foods with multiple health benefits. Various species of microalgae and cyanobacteria are used to supplement existing feedstocks. In this review, microalgae have been defined as a potential feedstock for domestic animals due to their abundance of proteins, carbohydrates, lipids, minerals, vitamins, and other high-value products. Additionally, the positive physiological effects on products of animals fed with microalgal biomass have been compiled and recommendations are listed to enhance the assimilation of biomolecules in ruminant and nonruminant animals, which possess differing digestive systems. Furthermore, the role of microalgae as prebiotics is also discussed. With regards to large scale cultivation of microalgae for use as feed, many economic trade-offs must be considered such as the selection of strains with desired nutritional properties, cultivation systems, and steps for downstream processing. These factors are highlighted with further investigations needed to reduce the overall costs of cultivation. Finally, this review outlines the pros and cons of utilizing microalgae as a supplementary feedstock for poultry and cattle, existing cultivation strategies, and the economics of large-scale microalgal production.
Effects of root dominate over aboveground litter on soil microbial biomass in global forest ecosystems
Authors: Jing Yanli , Tian Peng , Wang Qingkui , Li Weibin , Sun Zhaolin , Yang Hong 
Published: June 2021

Abstract
Background
Inputs of above- and belowground litter into forest soils are changing at an unprecedented rate due to continuing human disturbances and climate change. Microorganisms drive the soil carbon (C) cycle, but the roles of above- and belowground litter in regulating the soil microbial community have not been evaluated at a global scale.

Methods
Here, we conducted a meta-analysis based on 68 aboveground litter removal and root exclusion studies across forest ecosystems to quantify the roles of above- and belowground litter on soil microbial community and compare their relative importance.

Results
Aboveground litter removal significantly declined soil microbial biomass by 4.9% but root exclusion inhibited it stronger, up to 11.7%. Moreover, the aboveground litter removal significantly raised fungi by 10.1% without altering bacteria, leading to a 46.7% increase in the fungi-to-bacteria (F/B) ratio. Differently, root exclusion significantly decreased the fungi by 26.2% but increased the bacteria by 5.7%, causing a 13.3% decrease in the F/B ratio. Specifically, root exclusion significantly inhibited arbuscular mycorrhizal fungi, ectomycorrhizal fungi, and actinomycetes by 22.9%, 43.8%, and 7.9%, respectively. The negative effects of aboveground litter removal on microbial biomass increased with mean annual temperature and precipitation, whereas that of root exclusion on microbial biomass did not change with climatic factors but amplified with treatment duration. More importantly, greater effects of root exclusion on microbial biomass than aboveground litter removal were consistent across diverse forest biomes (expect boreal forests) and durations.

Conclusions
These data provide a global evidence that root litter inputs exert a larger control on microbial biomass than aboveground litter inputs in forest ecosystems. Our study also highlights that changes in above- and belowground litter inputs could alter soil C stability differently by shifting the microbial community structure in the opposite direction. These findings are useful for predicting microbe-mediated C processes in response to changes in forest management or climate.
The Wire Study—a protocol for a multi-stage feasibility study evaluating K-wire fixation of hand fractures in the UK
Authors: Wormald Justin Conrad Rosen , Mikhail Mark Medhat , Rodrigues Jeremy Neil , Gardiner Sonya , Pezas Theodore , Lloyd-Hughes Hawys , Issa Fadi , Jain Abhilash , Gardiner Matthew David 
Published: June 2021

Abstract
Background
Hand fractures are common and sometimes require surgery to restore function. Placement of Kirschner wires (K-wires) is the most common form of surgical fixation. After placement, a key decision is whether to bury the end of a K-wire or leave it protruding from the skin (exposed). A recent systematic review found no evidence to support either approach and a national clinician and surgeon survey demonstrated further uncertainty. We aim to determine the design of a definitive randomised controlled trial assessing the cost and clinical effectiveness of buried versus exposed Kirschner wires for adults with metacarpal or phalangeal fractures.

Methods
We will employ three methodologies: a national service evaluation of current clinical practice, patient and surgeon focus groups and a consensus meeting to finalise the protocol for a randomised controlled trial. For the service evaluation, all outcomes will be summarised using descriptive statistics overall and split by group (buried versus exposed K-wires). Information collected in the patient focus groups will be analysed thematically. The surgeon consensus meeting will address each part of the design in turn and through discussion agree a final protocol.

Discussion
The study may be monitored, or audited in accordance with the current approved protocol, Good Clinical Practice (GCP), relevant regulations and standard operating procedures. The Chief Investigator will submit and, where necessary, obtain approval from the above parties for all substantial amendments to the original approved documents. A feasibility study report will be published by the Wire Study Steering committee. Additional members of the steering group and citable collaborators will be listed within the manuscript and their roles identified.
Formalising the induction of patient and public involvement contributors on trial oversight committees
Authors: Pickering Emily C. , Hanley Bec , Bell Philip , Gath Jacqui , Hanlon Patrick , Oldroyd Robert , Stephens Richard , Tweed Conor D. 
Published: June 2021

AbstractBackgroundClinical Trials Units are encouraged to integrate Patient and Public Involvement (PPI) into all aspects of trial design, running and oversight. This research explored the induction and training of PPI Contributors joining trial oversight committees and was used to update the Medical Research Council Clinical Trials Unit at University College London’s (MRC CTU at UCL) induction pack for new PPI Contributors.MethodsPublished and unpublished materials provided by other CTUs and research organisations on training for PPI Contributors on oversight committees were reviewed, with themes then triangulated to identify the most common topics covered in induction training. A face-to-face workshop with PPI Contributors from the MRC CTU at UCL reviewed a draft updated Induction Pack. Findings from these discussions were incorporated into a revised induction pack which was then re-reviewed by the workshop attendees.ResultsNo published literature on this subject was found. However, several common themes were identified from unpublished materials. Workshop attendees agreed with most of the themes suggested in the initial draft pack based on the literature search and also provided a number of additional topics for discussion.ConclusionsThere is very little consistency in the induction of PPI Contributors on oversight committees. Whilst most local guidance explains the general role of a PPI Contributor, more context and background of the particular trial needs to be provided to allow for adequate induction of new committee members. The Induction Pack created provides a framework upon which trial managers can build a full picture of their study.
Humanitarian support in a denial of access context: emergent strategies at the interface of humanitarian and sovereign law
Authors: Biersteker Erwin , Ferguson Julie , Groenewegen Peter , Boersma Kees 
Published: June 2021

AbstractThe principles of international humanitarian law (IHL) have evoked considerable debate in the practice of humanitarian support, particularly in terms of emerging tensions with sovereign (national) law. Drawing on organization studies, we examine the emergent strategies aimed at resolving the ambiguous legal context in which humanitarian support operations in a conflict context are embedded. Our analysis of two missions revealed two types of emergent strategies, namelynetworkandnegotiationstrategies, differentiated by particular contextual dimensions. We extend the humanitarian law debate by showing the strategic interplay between the operational humanitarian context and international humanitarian principles, thereby connecting the fields of international law and organization science.
Talent management related concepts and debates in container shipping industry by an emerging market perspective
Authors: Yildiz Ramazan Özkan , Esmer Soner 
Published: June 2021

AbstractTalent management (TM) is referred as a young and developing field and it is claimed to be require more contribution from the different industry and country perspectives. Because of the highly dynamic and competitive nature of the container shipping industry, possession of valuable and unique human capital assets is evaluated as a substantial necessity to achieve business objectives and sustained competitive advantage by container shipping companies. Accordingly, TM is considered to be an essential factor contributing the accomplishment of these goals and the successful implementation of corporate strategy. Therefore, this study aims to contribute the development of TM field by investigating and evaluating TM related concepts and debates in Turkish container shipping industry. Through this extent, a combined method of content analysis with in-depth interview, has been organized to analyse TM oriented subjects in container shipping industry.
First record of a parasitoid, Megaselia (M) scalaris (Diptera: Phoridae) of fall armyworm, Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae) from India
Authors: Deshmukh Sharanabasappa S. , Kiran S. , Naskar Atanu , Pradeep Palam , Kalleshwaraswamy C. M. , Sharath K. N. 
Published: June 2021

AbstractThe fall armyworm (FAW), Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae), has become a major threat in maize cultivation since its invasion to India in 2018. The humpbacked fly, Megaselia scalaris (Loew) (Diptera: Phoridae), was recorded as a laboratory parasitoid of FAW, for the first time in India. Initially, 30–40 maggots of M. (M) scalaris emerged out from the dead pre-pupa and pupa of laboratory-reared FAW. The fly laid up to 15 eggs on the outer surface of 6th instar larva or pre-pupa of the FAW. The incubation period was 1–2 days. The fly had 3 larval instars which lasted 3–4 days and a pupal period of 10–11 days. The adults survived for 6–7 days.
High frequency AC electric glow discharge visualization technology and application in big diameter hypersonic low-density wind tunnel
Authors: Jiang Wanqiu , Qiu Huacheng , Yang Yanguang , Shi Yilei , Wang Jie , Li Jie , Long Zhengyi , Mao Chunman 
Published: June 2021

AbstractIn order to visualize the rarefied flow field in a Φ1m hypersonic low-density wind tunnel, an electric glow discharge technique based on high frequency excited power has been developed. Firstly, finite element simulation analysis has been carried out, and it is concluded that the breakdown voltage can be reduced by using high frequency power supply; then an electric glow discharge apparatus has been fabricated, and the discharge images were compared with simulations; besides, a clear flow field around HB-1 standard model has been observed using this technology; finally, the influence of glow discharge on the flow field, as well as the differences between direct current and high frequency electric glow discharges is discussed at the end of this paper.