bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Master Hoof (Claw) Care Program: Summary Statistics From Three Years
Authors: Shearer Jan K. , Van Amstel S. R. , Gonzalez-Sagues A. , Shearer L. C. 
Published: March 2021

In 1996, through a grant from the Florida dairy farmers, the Master Hoof Care Program was developed at the University of Florida. The objective of this program was (and is) to train dairy health care technicians and others in proper methods of foot care and claw trimming. The program is offered three times per year at the University of Florida and once annually at the University of Tennessee. This document is VM145, one of a series of the College of Veterinary Medicine-Large Animal Clinical Sciences, Florida Cooperative Extension Service, Institute of Food and Agricultural Sciences, University of Florida. Original publication date August 7, 2002.
https://edis.ifas.ufl.edu/vm109
Using "Ag in the Classroom" curricula: Teachers' awareness, attitudes and perceptions of agricultural literacy
Authors: Malecki Cynthia L. , Israel Glenn D. , Toro Elena 
Published: March 2021

Florida's residents are more dependent than ever on agriculture, but for many the relationship is invisible. This is because it is masked by the proliferation of widely available food products on store shelves and numerous intermediaries in the food processing, distribution and marketing system. The mission of agricultural education today is to create an awareness of this progression as well as the need for the agricultural industry. Additionally, agricultural education should create awareness about how newly developed technologies have become an integral part of the world's production of food and fiber. This document is AEC 370, a publication of the Agricultural Education and Communication Department, Florida Cooperative Extension Service, Instituteof Agricultural and Food Sciences, University of Florida. Publication date January, 2004.
Weed Control in Parsley
Authors: Stall William M. 
Published: March 2021

Parsley is placed in the "leafy greens" EPA group due to its closeness to lettuce and spinach for residues. Bensulide may be applied to parsley preplant incorporated or preemergence on mineral soils. This document is HS952, one of a series of the Horticultural Sciences Department, Florida Cooperative Extension Service, Institute of Food and Agricultural Sciences, University of Florida. Publication date: August 2003.
HS952/WG212: Weed Management in Parsley and Cilantro (ufl.edu)
PELUANG TERJADINYA PENGANGGURAN DI PROVINSI BENGKULU : SEBERAPA BESAR?
Authors: Marini Lisa , Putri Novi Tri 
Published: March 2021

The aim of this research is to analyze how are opportunity occured unemployment based on characteristics of populations in Bengkulu province. Characteristics of populations that used are education, training/course certificate, age, gender, work experience, marital status, status in the family, and area of residence. The analytical method used to achieve this goal is the logistic regression analysis. Type of key data use row data derived from the National Labor Force Survey (Sakernas) February 2018. The result of the calculation using the program SPSS 20.0 was found that not all the variables of population characteristics significantly affect the chances of unemployment with a confidence level of 95 percent. Education and age are significantly affect of unemployment in Bengkulu Province, while training/course certificate, gender, work experience, marital status, status in the family and area residence are not significantly affect of unemployment in Bengkulu Province. Keywords: Logistic Regression Analysis, National Labor Force Survey (Sakernas), Unemployment.
PROBLEM MENDASAR PENERAPAN BEA PEROLEHAN HAK ATAS TANAH DAN/ATAU BANGUNAN
Authors: Faishal Achmad 
Published: March 2021

Pentingnya artikel ini adalah untuk menunjukkan bahwa penerapan Pajak Bea Perolehan Hak Atas Tanah dan/atau Bangunan (BPHTB) terdapat problem mendasar, Pertama, ditengah masyakarakat terdapat suatu keadaan ketidaktahuan bahwa perolehan hak atas tanah dan/atau bangunan terkena kewajiban pajak, hal itu terjadi akibat ketiadaan informasi publik yang menjadi hak dari publik untuk mengetahuinya. Kedua, dalam eksistensi pemungutannya saat ini, ternyata besaran pungutannya berdasarkan pada regresi dasar nilai perolehan objek pajak yang oleh aturan bisa menggunakan harga transaksi jual beli atau Nilai Jual Objek Pajak pada Pajak Bumi dan Bangunan (NJOP PBB) tahun perolehannya. Hal demikian menimbulkan adanya ketidakpastian hukum. Ketiga, peralihan hak atas tanah dan/atau bangunan kekuatannya dipengaruhi aturan keharusan dihadapan Pejabat Pembuat Akta Tanah/Notaris (PPAT/Notaris) dan akta peralihan hak hanya akan ditandatangani oleh Notaris apabila Bea Perolehan Hak Atas Tanah dan/atau Bangunan telah dibayar. Karena adanya regresi dasar nilai perolehan objek Pajak dapat menimbulkan perbedaan besaran nilai Pajak, maka PPAT/Notaris cenderung melibatkan diri untuk mengarahkan pada bentuk transaksi dengan menurunkan harga jual beli agar nilai Pajak tidak sebesar yang semestinya. Kondisi-kondisi tersebut dikemukakan dalam artikel ini secara dialektis sebagai respon terhadap kondisi aturan hukum.
Statin Use and Benefits of Thyroid Function: A Retrospective Cohort Study
Authors: Wang Yupeng , Li Qihang , Yuan Zhongshang , Ma Shizhan , Shao Shanshan , Wu Yafei , Wang Zhixiang , Li Qiu , Gao Ling , Zhao Meng , Zhao Jiajun 
Published: March 2021

PurposePrevious studies have suggested that cholesterol may influence thyroid function. Since statins are widely used for their cholesterol-lowering effect, we aimed to assess the association between statin use and thyroid function, and also to explore the role of the cholesterol-lowering effect in it.MethodsWe performed a retrospective cohort study derived from REACTION study. Eligible subjects receiving statin therapy were included in the statin group, and sex-, age-, total cholesterol (TC)-, and thyroid function-matched participants without lipid-lowering therapy were included in the control group. The median follow-up time was three years. Outcomes of thyroid function were evaluated at the end of follow-up. We used multivariable regression models to assess the association between statin use and outcomes of thyroid function, and also performed mediation analyses to explore the role of cholesterol in it.ResultsA total of 5,146 participants were screened, and 201 eligible subjects in the statin group and 201 well-matched subjects in the control group were analyzed. At the end of follow-up, TC and thyroid-stimulating hormone (TSH) levels in the statin group were lower than those in the control group (both p &lt; 0.05), and the percentage of euthyroid subjects was higher in the statin group (88.06% vs. 76.12%, p = 0.002). The incidence rate of subclinical hypothyroidism (SCH) in euthyroid subjects was lower in the statin group (6.29% vs. 14.86%, p = 0.009), and the remission rate among subjects with SCH was higher in the statin group (50.00% vs. 15.38%, p = 0.008). In multivariable regression analyses, statin use was independently associated with lower TSH levels and higher odds to be euthyroid (OR 2.335, p = 0.004) at the end of follow-up. Mediation analyses showed the association between statin use and TSH levels were mediated by TC changes during follow-up.ConclusionStatin use was associated with benefits of thyroid function, and TC changes serve as a mediator of the association between statin use and TSH levels. Further studies are needed to clarify the possible underlying mechanism.
Effects of Deep Brain Stimulation in the Subthalamic Nucleus on Neurocognitive Function in Patients With Parkinson's Disease Compared With Medical Therapy: A Meta-Analysis
Authors: Wang Jiazhen , Pan Ru , Cui Ying , Wang Zhigang , Li Qinghua 
Published: March 2021

Background: DBS has been shown to significantly affect motor symptoms in Parkinson's disease (PD). However, some studies have suggested that it may have adverse effects on patients' neurocognitive function. To clarify this operation's effect on neurocognitive function, we collected studies containing neurocognitive function evaluation for qualitative and quantitative analysis.Methods: We searched relevant clinical studies through Pubmed and Embase databases and extracted and sorted out information such as sample size, post-operative scores, pre-operative and post-operative evaluation interval, PD course, and exclusion criteria, from articles meeting the standards. The magnitude and variance of the DBS group's combined effects and the drug therapy group in each neurocognitive domain were calculated and analyzed by the random-effects model.Results: Compared with the drug treatment group, the verbal fluency of patients in the experimental group was significantly decreased at least moderately (ES = −0.553), in which the phonemic fluency declines greatly (ES = −0.842), learning and memory ability was slightly decreased (ES = −0.305), and other neurocognitive functions were not significantly decreased.Conclusion: STN-DBS can affect verbal fluency and damage learning and memory. There was no significant correlation between the above effects and disease progression itself, and it was more likely to be associated with STN-DBS. It is suggested that post-operative patients should be trained and evaluated regularly for their verbal fluency and learning and memory ability. The safety of STN-DBS is acceptable for the majority of patients with motor symptoms.
DRP1 Promotes BRAFV600E-Driven Tumor Progression and Metabolic Reprogramming in Colorectal Cancer
Authors: Padder Rayees Ahmad , Bhat Zafar Iqbal , Ahmad Zaki , Singh Neetu , Husain Mohammad 
Published: March 2021

BackgroundMitochondria are highly dynamic organelles which remain in a continuous state of fission/ fusion dynamics to meet the metabolic needs of a cell. However, this fission/fusion dynamism has been reported to be dysregulated in most cancers. Such enhanced mitochondrial fission is demonstrated to be positively regulated by some activating oncogenic mutations; such as those of KRAS (Kristen rat sarcoma viral oncogene homologue) or BRAF (B- rapidly accelerated fibrosarcoma), thereby increasing tumor progression/ chemotherapeutic resistance and metabolic deregulation. However, the underlying mechanism(s) are still not clear, thus highlighting the need to further explore possible mechanism(s) of intervention. We sought to investigate how BRAFV600E driven CRC (colorectal cancer) progression is linked to mitochondrial fission/fusion dynamics and whether this window could be exploited to target CRC progression.MethodsWestern blotting was employed to study the differences in expression levels of key proteins regulating mitochondrial dynamics, which was further confirmed by confocal microscopy imaging of mitochondria in endogenously expressing BRAFWT and BRAFV600E CRC cells. Proliferation assays, soft agar clonogenic assays, glucose uptake/lactate production, ATP/ NADPH measurement assays were employed to study the extent of carcinogenesis and metabolic reprograming in BRAFV600E CRC cells. Genetic knockdown (shRNA/ siRNA) and/or pharmacologic inhibition of Dynamin related protein1/Pyruvate dehydrogenase kinase1 (DRP1/PDK1) and/or BRAFV600E were employed to study the involvement and possible mechanism of these proteins in BRAFV600E driven CRC. Statistical analyses were carried out using Graph Pad Prism v 5.0, data was analyzed by unpaired t-test and two-way ANOVA with appropriate post hoc tests.ResultsOur results demonstrate that BRAFV600E CRC cells have higher protein levels of mitochondrial fission factor- DRP1/pDRP1S616 leading to a more fragmented mitochondrial state compared to those harboring BRAFWT. This fragmented mitochondrial state was found to confer glycolytic phenotype, clonogenic potential and metastatic advantage to cells harboring BRAFV600E. Interestingly, such fragmented mitochondrial state seemed positively regulated by mitochondrial PDK1 as observed through pharmacologic as well as genetic inhibition of PDK1.ConclusionIn conclusion, our data suggest that BRAFV600E driven colorectal cancers have fragmented mitochondria which confers glycolytic phenotype and growth advantage to these tumors, and such phenotype is dependent at least in part on PDK1- thus highlighting a potential therapeutic target.
A Machine Learning Model Based on PET/CT Radiomics and Clinical Characteristics Predicts ALK Rearrangement Status in Lung Adenocarcinoma
Authors: Chang Cheng , Sun Xiaoyan , Wang Gang , Yu Hong , Zhao Wenlu , Ge Yaqiong , Duan Shaofeng , Qian Xiaohua , Wang Rui , Lei Bei , Wang Lihua , Liu Liu , Ruan Maomei , Yan Hui , Liu Ciyi , Chen Jie , Xie Wenhui 
Published: March 2021

ObjectivesAnaplastic lymphoma kinase (ALK) rearrangement status examination has been widely used in clinic for non-small cell lung cancer (NSCLC) patients in order to find patients that can be treated with targeted ALK inhibitors. This study intended to non-invasively predict the ALK rearrangement status in lung adenocarcinomas by developing a machine learning model that combines PET/CT radiomic features and clinical characteristics.MethodsFive hundred twenty-six patients of lung adenocarcinoma with PET/CT scan examination were enrolled, including 109 positive and 417 negative patients for ALK rearrangements from February 2016 to March 2019. The Artificial Intelligence Kit software was used to extract radiomic features of PET/CT images. The maximum relevance minimum redundancy (mRMR) and least absolute shrinkage and selection operator (LASSO) logistic regression were further employed to select the most distinguishable radiomic features to construct predictive models. The mRMR is a feature selection method, which selects the features with high correlation to the pathological results (maximum correlation), meanwhile retain the features with minimum correlation between them (minimum redundancy). LASSO is a statistical formula whose main purpose is the feature selection and regularization of data model. LASSO method regularizes model parameters by shrinking the regression coefficients, reducing some of them to zero. The feature selection phase occurs after the shrinkage, where every non-zero value is selected to be used in the model. Receiver operating characteristic (ROC) analysis was used to evaluate the performance of the models, and the performance of different models was compared by the DeLong test.ResultsA total of 22 radiomic features were extracted from PET/CT images for constructing the PET/CT radiomic model, and majority of these features used were based on CT features (20 out of 22), only 2 PET features were included (PET percentile 10 and PET difference entropy). Moreover, three clinical features associated with ALK mutation (age, burr and pleural effusion) were also employed to construct a combined model of PET/CT and clinical model. We found that this combined model PET/CT-clinical model has a significant advantage to predict the ALK mutation status in the training group (AUC = 0.87) and the testing group (AUC = 0.88) compared with the clinical model alone in the training group (AUC = 0.76) and the testing group (AUC = 0.74) respectively. However, there is no significant difference between the combined model and PET/CT radiomic model.ConclusionsThis study demonstrated that PET/CT radiomics-based machine learning model has potential to be used as a non-invasive diagnostic method to help diagnose ALK mutation status for lung adenocarcinoma patients in the clinic.
Adipocytes Under Obese-Like Conditions Change Cell Cycle Distribution and Phosphorylation Profiles of Breast Cancer Cells: The Adipokine Receptor CAP1 Matters
Authors: Bergqvist Malin , Elebro Karin , Borgquist Signe , Rosendahl Ann H. 
Published: March 2021

BackgroundObesity and associated metabolic conditions impact adipocyte functionality with potential consequences for breast cancer risk and prognosis, but contributing mechanisms remain to be understood. The adipokine receptor adenylyl cyclase-associated protein-1 (CAP1) has been implicated in the progression of breast cancer, but results are conflicting and the underlying molecular mechanisms are still unknown. In this study, molecular and cellular effects in breast cancer cells by stimulation of adipocytes under normal or obese-like conditions, and potential involvement of CAP1, were assessed.Material and MethodsEstrogen receptor (ER)-positive T47D and ER-negative MDA-MB-231 breast cancer cells were exposed to adipocyte-secretome from adipocytes placed under pressures mimicking normal and obese-like metabolic conditions. Changes in phosphorylated kinase proteins and related biological pathways were assessed by phospho-antibody array and PANTHER analysis, cell proliferation were investigated through sulforhodamine B, cell cycle distribution by flow cytometry. Functional effects of CAP1 were subsequently examined following small interfering (si)RNA-mediated knockdown.ResultsProtein phosphorylations involved in important biological processes were enriched in T47D breast cancer cells in response to adipocyte secretome from obese-like compared with normal conditions. The obesity-associated adipocyte secretome further stimulated cell proliferation and a shift from cell cycle G1-phase to S- and G2/M-phase was observed. Silencing of CAP1 decreased cell proliferation in both T47D and MDA-MB-231 cells, and reduced the obesity-associated secretome-induction of phosphoproteins involved in cell proliferation pathways.ConclusionsThese results indicate that the adipocyte secretome and CAP1 are mechanistically important for the proliferation of both ER-positive and ER-negative breast cancer cells, and potential signaling mediators were identified. These studies provide biological insight into how obesity-associated factors could affect breast cancer.
Fecal Multidimensional Assay for Non-Invasive Detection of Colorectal Cancer: Fecal Immunochemical Test, Stool DNA Mutation, Methylation, and Intestinal Bacteria Analysis
Authors: Mo Shaobo , Wang Hui , Han Lingyu , Xiang Wenqiang , Dai Weixing , Zhao Pengfei , Pei Fengchun , Su Zhixi , Ma Chengcheng , Li Qi , Wang Zhimin , Cai Sanjun , Wang Hao , Liu Rui , Cai Guoxiang 
Published: March 2021

BackgroundFecal immunochemical test (FIT), DNA mutation, DNA methylation, and microbial dysbiosis all showed promising in colorectal cancer (CRC) non-invasive detection. We assessed CRC detection with an assay combining all these strategies and investigated the effect of clinical features on the performance of this comprehensive test.MethodsWe performed a multidimensional analysis study using stool samples collected from 108 patients with CRC, 18 patients with colorectal adenoma, and 36 individuals with no evidence of colorectal disease. The multidimensional analysis of stool samples including FIT, stool DNA (sDNA) tests for three methylated genes (Septin9, NDRG4, BMP3) and three mutated genes (KRAS, BRAF, PI3KCA) using next generation sequencing as well as detection of stool bacteria level of Fusobacterium nucleatum and Parvimonas micra using qPCR method. We used a linear support vector classification model to analyze the data.ResultsThe sensitivity of FIT alone was 69.4% for CRC and 11.1% for adenoma. Separately, the sensitivity of the detection of intestinal bacteria, DNA mutation, and DNA methylation for CRC was 58.3, 50.0, and 51.9%, respectively. The combination of FIT and sDNA tests had a sensitivity of 81.5% for CRC (AUC: 0.93, better than FIT alone, P = 0.017) and 27.8% for adenoma with 94.4% specificity. Sensitivity of the multidimensional test to detect CRC with stage II (84.6%) and III (91.9%) CRC was relatively higher (88.2%) than that of patients with stage I (60.0%) and stage IV (75.0%) (P = 0.024). The rate of CRC detection increased with tumor size (P = 0.008) and age (P = 0.04). Interestingly, the rate of CRC detection was higher in smoking persons than non-smokers with marginal significance (P = 0.08).ConclusionsThe multidimensional assay of stool samples combining FIT and stool DNA tests further improved the diagnostic sensitivity for CRC. This could provide new approach for improvement of CRC screening and further demonstrations are warranted.
Evaluation of a New Tubular Finger Oxygen-Enriched Oil Inside-Coated Dressing Device in Pediatric Patients Undergoing Distal Hypospadias Repair: A Prospective Randomized Clinical Trial Part II
Authors: Esposito Ciro , Coppola Vincenzo , Del Conte Fulvia , Cerulo Mariapina , Esposito Giovanni , Crocetto Felice , Castagnetti Marco , Calignano Antonio , Escolino Maria 
Published: March 2021

Background: This study was the second part of a prospective randomized clinical trial and aimed to evaluate the use of a tubular finger oxygen-enriched oil inside-coated dressing device and its effect on the post-operative outcome of children undergoing distal hypospadias repair.Methods: A prospective single-blinded randomized clinical trial was carried out between September 2019 and September 2020. We included all patients with distal hypospadias, who received Snodgrass urethroplasty and preputioplasty. The patients were randomized in two groups according to the type of dressing: tubular finger oxygen-enriched oil inside-coated device (G1) and elastic net bandage with application of oxygen-enriched oil-based gel (G2). The patients were evaluated at 7, 14, 21, 30, and 60 post-operative day (POD).Results: Sixty-four patients (median age 14 months) were included in the study and randomized in two groups, each of 32 patients. Post-operative preputial edema rate was significantly lower in G1 (3/32, 9.3%) compared with G2 (10/32, 31.2%) (p = 0.001). The median duration of preputial edema was significantly shorter in G1 compared with G2 (6 vs. 10.5 days) (p = 0.001). Penile diameter measurements at 4th, 7th, 14th POD proved that entity and duration of post-operative swelling were objectively decreased using the new dressing. The wound healing was significantly faster in G1 compared with G2 (14.2 vs. 18.5 days) (p = 0.001). The post-operative complications rate was significantly lower in G1 (0%) compared with G2 (3/32, 9.3%) (p = 0.001). Foreskin dehiscence occurred in two G2 patients (6.2%) whereas, breakdown of urethroplasty and preputioplasty occurred in one G2 patient (3.1%) due to scratching injuries. The dressing management was subjectively assessed by nurses to be easier in G1 patients compared with G2 ones (median score 1.2 vs. 3.5) (p = 0.001). The median treatment costs were significantly lower in G1 compared with G2 (55 vs. 87 eur) (p = 0.001). No adverse skin reactions occurred.Conclusions: Post-operative dressing using tubular finger oxygen-enriched oil inside-coated device was highly effective, easy to manage, cheaper and associated with a lower rate of foreskin and urethral complications compared with the standard dressing method in pediatric patients undergoing distal hypospadias repair. It was also clinically safe without allergy or intolerance to the product.