bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Novel Propidium Monoazide-Based PCR Assay Can Measure Viable Uropathogenic E. coli in Vitro and in Vivo
Authors: Lee Albert S , Lamanna Olivia K , Ishida Kenji , Hill Elaise , Hsieh Michael H. 
Published: October 2021

AbstractBackgroundPolymerase chain reaction (PCR) is an important means by which to study the urine microbiome and is emerging as possible alternative to urine cultures to identify pathogens that cause urinary tract infection (UTI). However, PCR is limited by its inability to differentiate DNA originating from viable, metabolically active versus non-viable, inactive bacteria. This drawback has led to concerns that urobiome studies and PCR-based diagnosis of UTI are confounded by the presence of relic DNA from non-viable bacteria in urine. Propidium monoazide (PMA) dye can penetrate cells with compromised cell membranes and covalently bind to DNA, rendering it inaccessible to amplification by PCR. Although PMA has been shown to differentiate between non-viable and viable bacteria in various settings, its effectiveness in urine has not been previously studied. We sought to investigate the ability of PMA to differentiate between viable and non-viable bacteria in urine.MethodsVarying amounts of viable or non-viable uropathogenic E. coli (UTI89) or buffer control were titrated with mouse urine. The samples were centrifuged to collect urine sediment or not centrifuged. Urine samples were incubated with PMA and DNA cross-linked using blue LED light. DNA was isolated and uidA gene-specific PCR was performed. For in vivo studies, mice were inoculated with UTI89, followed by ciprofloxacin treatment or no treatment. After the completion of ciprofloxacin treatment, an aliquot of urine was plated on non-selective LB agar and another aliquot was treated with PMA and subjected to uidA-specific PCR.ResultsPMA’s efficiency in excluding DNA signal from non-viable bacteria was significantly higher in bacterial samples in phosphate-buffered saline (PBS, dCT=13.69) versus bacterial samples in unspun urine (dCT=1.58). This discrepancy was diminished by spinning down urine-based bacterial samples to collect sediment and resuspending it in PBS prior to PMA treatment. In 3 of 5 replicate groups of UTI89-infected mice, no bacteria grew in culture; however, there was PCR amplification of E. coli after PMA treatment in 2 of those 3 groups.ConclusionWe have successfully developed PMA-based PCR methods for amplifying DNA from live bacteria in urine. Our results suggest that non-PMA bound DNA from live bacteria can be present in urine, even after antibiotic treatment.This indicates that viable but non-culturable E. coli can be present following treatment of UTI, and may explain why some patients have persistent symptoms but negative urine cultures following UTI treatment.
Mitochondrial active Ras2 protein promotes apoptosis and regulated cell death in a cAMP/PKA pathway-dependent manner in budding yeast
Authors: Bonomelli Barbara , Martegani Enzo , Colombo Sonia 
Published: October 2021

AbstractIn previous papers, using the eGFP-RBD3 probe, which binds Ras-GTP with high affinity, we showed that activated Ras proteins are localized to the plasma membrane and in the nucleus in wild-type Saccharomyces cerevisiae cells growing exponentially on glucose, while an aberrant accumulation of activated Ras in mitochondria correlates to mitochondrial dysfunction, accumulation of ROS and an increase of apoptosis. In this paper, we show that lack of TPS1, which is known to trigger apoptosis in S. cerevisiae, induces localization of active Ras proteins in mitochondria, confirming the above-mentioned correlation. Next, by characterizing the ras1Δ and ras2Δ mutants concerning localization of active Ras proteins and propensity to undergo cell death, we show that active Ras2 proteins, which accumulate in the mitochondria following addition of acetic acid, a well-known pro-apoptotic stimulus, might be the GTPases involved in regulated cell death, while active Ras1 proteins, constitutively localized in mitochondria, might be involved in a pro-survival molecular machinery. Finally, by characterizing the gpa2Δ and cyr1Δ mutants concerning the propensity to undergo cell death, we show that active mitochondrial Ras proteins promote apoptosis through the cAMP/PKA pathway.
Kdm6b confers Tfdp1 with the competence to activate p53 signalling in regulating palatogenesis
Authors: Guo Tingwei , Han Xia , He Jinzhi , Feng Jifan , Jing Junjun , Janečková Eva , Lei Jie , Ho Thach-Vu , Xu Jian , Chai Yang 
Published: October 2021

AbstractEpigenetic regulation plays extensive roles in diseases and development. Disruption of epigenetic regulation not only increases the risk of cancer, but can also cause various developmental defects. However, it is still unclear how epigenetic regulators coordinate with tissue-specific regulatory factors during morphogenesis of specific organs. Using palatogenesis as a model, we reveal the functional significance of Kdm6b, a H3K27me3 demethylase, in regulating embryonic development. Our study shows that Kdm6b plays an essential role in neural crest development, and loss of Kdm6b disturbs p53 pathway-mediated activity, leading to complete cleft palate along with cell proliferation and differentiation defects. Furthermore, activity of H3K27me3 on the promoter of p53 is precisely controlled by Kdm6b, and Ezh2 in regulating p53 expression in cranial neural crest cells. More importantly, Kdm6b renders chromatin accessible to the transcription factor Tfdp1, which binds to the promoter of p53 along with Kdm6b to specifically activate p53 expression during palatogenesis. Collectively our results highlight the important role of the epigenetic regulator Kdm6b and how it cooperates with Tfdp1 to achieve its functional specificity in regulating p53 expression, and further provide mechanistic insights into the epigenetic regulatory network during organogenesis.
Can SMEs in the food industry expect competitive advantages from proactive CSR when CSR trade-offs exist?
Authors: Kim Yeonsoo , Bhalla Nandini 
Published: October 2021

PurposeThe study aims to examine the effects of proactive vs passive environmental corporate social responsibility (CSR) in the context of small and medium size enterprises (SMEs), factoring in the moderating effects of price and the mediating effects of company–consumer identification(C-C identification) on consumer responses.Design/methodology/approachAn experiment with general consumer samples was conducted. A randomized 2 (CSR levels: proactive CSR vs passive CSR) × 2 (price as a CSR trade-off: higher price vs lower price) full factorial design was used.FindingsThe study findings revealed that proactive environmental CSR not only engendered more positive C-C identification but also resulted in more favorable consumer attitudes, stronger supportive communication intent and purchase intent. In addition, when a company demonstrates proactive CSR, consumers' C-C identification is generally positive irrespective of price differences, and in turn, more positive reactions follow. When a company takes a passive approach and offers lower prices, respondents showed significantly less positive C-C identification, and less favorable responses. This indicates that passive environmental CSR programs can potentially backfire, especially when combined with lower prices. This study also shows the important mediating impact of C-C identification on consumer responses.Originality/valueThis study is one of the few to explore consumer perceptions of and reactions toward the food industry's environmental CSR programs by degree of CSR involvement and price differences in the context of SMEs. This study's findings provide useful information to SME managers and public relations practitioners who work closely with SMEs, allowing them to make informed strategic decisions, especially when they evaluate the extent of their company's commitment to environmentally proactive CSR practices and its communication to consumers.
Towards a meaningful innovation paradigm: conceptual framework and practice of leading world-class enterprise
Authors: Wang Luyao , Qu Guannan , Chen Jin 
Published: October 2021

Purpose
The purpose of this study is to conceptualize the paradigm of meaningful innovation (MI) by exploring the theoretical basis, identifying its core concepts and key processes, and supporting it with evidence from leading world-class enterprises.

Design/methodology/approach
Based on a systemic literature review on the evolution of innovation paradigms, this study builds a conceptual framework to explicate the core concepts and process of MI. Moreover, a horizontal embedded case study of GREE electric appliances is conducted to further enrich, refine and support the proposed framework.

Findings
The main finding of this study is that MI could be regarded as a process of integrating innovation elements (resources, capabilities, systems, etc.) with internal and external innovation meaning to obtain outcomes with both economic value and social significance. As a “long-termism” paradigm with meaning identification and conversion as its core, MI is driven by the collaboration of “deductive mechanism” and “reflective mechanism.”

Originality/value
Based on the previous studies concerning innovation paradigms on the internal and external meaning, this paper proposes an integrated framework of MI. In this framework, enterprises can consider internal and external meanings through deductive and reflective mechanisms, to better coordinate resources, capabilities, institutions, markets and other factors to achieve higher innovation performance.
Factors affecting the international competitiveness of polish economy system in 2004-2019
Authors: Rojek Konrad 
Published: October 2021

Purpose
This study aims to present the issue of the international systemic competitiveness of the Polish economy. The essence of this concept was shown, as well as the measures and methods of analysis used. The aim of the research was to identify the factors that had the greatest impact on the formation of the international systemic competitiveness of the Polish economy.

Design/methodology/approach
An econometric model was constructed to explain the shaping of the value of the dependent variable (gross domestic product [GDP] per capita) in the years 2004–2019. For this purpose, explanatory variables were used selected from among the measures of the international systemic competitiveness of the Polish economy. The developed econometric model was verified to check its practical usefulness. This process was performed using the Gretl program. The research also used the Pentagon Model of Macroeconomic Stabilization, which was used to examine the general economic development of Poland because of which it is possible to conclude about the international systemic competitiveness of the economy.

Findings
In the analyzed period (2004–2019), the international systemic competitiveness of the Polish economy was to the greatest extent conditioned by such factors as government integrity, tax burdens and investment freedom. It is significant that the integrity of the government had a negative impact on the value of GDP per capita.

Practical implications
The results of the conducted research may be particularly useful for the institutional sphere. They indicate systemic factors that had the greatest impact on the prosperity of Polish society in the analyzed period. This enables the weakest elements of the policy to be identified and improved. Proper applications and appropriate corrective actions will have a positive economic effect.

Originality/value
So far, it has not been possible to develop/indicate a uniform and generally accepted measure and method of analyzing international systemic competitiveness. Therefore, all attempts to assess and measure systemic competitiveness have a high research value. The vast majority of studies on the international competitiveness of the economy focus only on assessing its level (growth, decline and comparison with other countries). When building an econometric model (based on the 2004–2019 time series), the author also checks the impact of its individual components, not only its level. On this basis, it can be deduced, which factors influenced the competitiveness in a given period to a greater extent, positively or negatively.
Cybersecurity capabilities for critical infrastructure resilience
Authors: Malatji Masike , Marnewick Annlizé L. , Von Solms Suné 
Published: October 2021

PurposeFor many innovative organisations, Industry 4.0 paves the way for significant operational efficiencies, quality of goods and services and cost reductions. One of the ways to realise these benefits is to embark on digital transformation initiatives that may be summed up as the intelligent interconnectivity of people, processes, data and cyber-connected things. Sadly, this interconnectivity between the enterprise information technology (IT) and industrial control systems (ICS) environment introduces new attack surfaces for critical infrastructure (CI) operators. As a result of the ICS cybersecurity risk introduced by the interconnectivity between the enterprise IT and ICS networks, the purpose of this study is to identify the cybersecurity capabilities that CI operators must have to attain good cybersecurity resilience.Design/methodology/approachA scoping literature review of best practice international CI protection frameworks, standards and guidelines were conducted. Similar cybersecurity practices from these frameworks, standards and guidelines were grouped together under a corresponding National Institute of Standards and Technology (NIST) cybersecurity framework (CF) practice. Practices that could not be categorised under any of the existing NIST CF practices were considered new insights, and therefore, additions.FindingsA CI cybersecurity capability framework comprising 29 capability domains (cybersecurity focus areas) was developed as an adaptation of the NIST CF with an added dimension. This added dimension emphasises cloud computing and internet of things (IoT) security. Each of the 29 cybersecurity capability domains is executed through various capabilities (cybersecurity processes and procedures). The study found that each cybersecurity capability can further be operationalised by a set of cybersecurity controls derived from various frameworks, standards and guidelines, such as COBIT®, CIS®, ISA/IEC 62443, ISO/IEC 27002 and NIST Special Publication 800-53.Practical implicationsCI sectors are immediately able to adopt the CI cybersecurity capability framework to evaluate their levels of resilience against cyber-attacks, given new attack surfaces introduced by the interconnectivity of cyber-connected things between the enterprise and ICS levels.Originality/valueThe authors present an added dimension to the NIST framework for CI cyber protection. In addition to emphasising cryptography, IoT and cloud computing security aspects, this added dimension highlights the need for an integrated approach to CI cybersecurity resilience instead of a piecemeal approach.
Lean practices toward a balanced sustainability in higher education institutions: a Brazilian experience
Authors: Klein Leander Luiz , De Guimarães Julio Cesar Ferro , Severo Eliana Andréa , Dorion Eric Charles Henri , Schirmer Feltrin Thiago 
Published: October 2021

Purpose
As few studies have explored the causal relationship between both the triple bottom line (TBL) of sustainability and the lean practices in higher education institutions (HEIs), the purpose of this paper is to analyze the relationships between lean practices and sustainable practices in Brazilian HEIs.

Design/methodology/approach
A survey was conducted with 454 public and private HEIs workers in Brazil. Confirmatory factor analysis and structural equations modeling were used for data analysis.

Findings
The results support the hypotheses of the study that HEI lean practices are positively related to environmental, economic and social practices in HEIs.

Practical implications
HEI managers must evaluate the use of lean practices in the organizational processes, to identify their weaknesses and to improve the aspects that influence a balanced TBL of sustainability practices.

Social implications
The findings highlight the importance of leadership as a support for the workers, as a continuous improvement practice on a daily basis and a focus on the student as a basic principle of HEI success.

Originality/value
This article provides new measurement and structural models to analyze Lean thinking practices as the predictors of sustainability practices. The work could assist any HEI in prioritizing its strategies and actions and to contribute to excellence in decision-making. This study could contribute as a source of empirical data for transferability in other contexts for HEIs.
Key performance indicators for a sustainable recovery strategy in health-care supply chains: COVID-19 pandemic perspective
Authors: Rahman Towfique , Moktadir Md. Abdul , Paul Sanjoy Kumar 
Published: October 2021

Purpose
Supply chains’ (SCs’) sustainability practices and recovery strategies are attaining popularity in academia and industries to improve the resilience of the SCs and to manage large-scale disruptions. The global pandemic caused by the COVID-19 has raised the question of the sustainability of essential health-care products’ SCs of Bangladesh. It is an essential avenue for making the life of people safe and secure. Despite its importance, most of the health-care SCs in Bangladesh are struggling to meet the demand of its nation due to capacity shortage, technological backwardness of the manufacturers, delivery capacity shortages and less advanced forecasting capabilities. Therefore, this study aims to investigate the key performance indicators (KPIs) of a sustainable recovery strategy in the context of health-care SCs considering the COVID-19 pandemic.

Design/methodology/approach
This study used a dynamic method named graph theory and matrix approach to evaluate the most critical KPIs of a sustainable recovery strategy in the context of Bangladeshi health-care SCs.

Findings
The result revealed that dynamic forecasting and planning with a smooth delivery and distribution support system, production capacity diversification and having alternative or multiple suppliers during extraordinary disruptions may aid in the sustainability of the health-care SCs in Bangladesh.

Originality/value
This study is unique as no previous study has identified and examined the sustainable recovery strategy perspective KPIs considering the COVID-19 pandemic in the context of Bangladeshi health-care SCs. This study will also add value by guiding decision-makers of the health-care SCs of Bangladesh to adopt strategies toward the sustainability of SCs.
The sustainability reporting-firm performance nexus: evidence from a threshold model
Authors: Bansal Manish , Samad Taab Ahmad , Bashir Hajam Abid 
Published: October 2021

PurposeThis study aims to provide a convincing argument behind the mixed findings on the association between sustainability reporting and firm performance by investigating the possibility of a non-linear relationship through a threshold model.Design/methodology/approachThis study used (Hansen’s 1999) threshold framework to investigate the relationship between firm performance and sustainability reporting using a sample of 210 Bombay Stock Exchange-listed firms spanning over 10 years from March 2010 to March 2019. This framework helps to test the threshold effect’s presence, estimate the threshold value and check the authenticity of the estimated threshold value.FindingsSustainability reporting has a differential threshold impact on the different indicators of firm performance. On the one hand, the authors’ results illustrate that the firms’ operating performance is positively impacted if and only if the sustainability reporting crosses a certain threshold. On the other hand, sustainability reporting positively impacts firms’ market performance only up to a cut-off point.Practical implicationsManagers should strive to balance sustainability reporting to reap its desired benefits on firm performance.Originality/valueThis study explores the possible non-linearity in the association between firm performance and sustainability reporting and explains the relationship’s inconclusive results. Further, this study explores the field in the novel emerging economy with unique institutional settings that mandate spending on sustainability activities.
Shear resistance of sandwich panel connection at elevated temperature
Authors: Cábová Kamila , Garifullin Marsel , Shoushtarian Mofrad Ashkan , Wald František , Mela Kristo , Ciupack Yvonne 
Published: October 2021

PurposeSandwich construction has developed and has become an integral part of lightweight construction. In the recent projects, it has been shown that by using sandwich panels as stabilizing members, a considerable amount of savings of steel can be achieved for structural members at ambient temperature. These stabilizing effects may also help to achieve similar savings in case of fire.Design/methodology/approachThe response of a sandwich single panel as well as the behaviour of the whole structure at ambient temperature and in case of fire is influenced by joints between the sandwich panels and the sub-structure. The fastenings used to fix the sandwich panels to a sub-structure may be loaded by shear forces caused by self-weight, live loads or diaphragm action. Therefore, an experimental investigation was conducted to investigate the shear behaviour of sandwich panel joints in fire.FindingsThis paper summarized briefly the experimental results, numerical simulations and analytical models on the shear behaviour of sandwich panel joints at ambient and elevated temperatures.Research limitations/implicationsThe work is limited to studied types of screws and sandwich panels which are generally used in current sandwich construction.Practical implicationsThese stabilizing effects in sandwich construction help to achieve savings in case of fire.Social implicationsSandwich construction has developed and has become an integral part of lightweight construction. In the recent projects, it has been shown that by using sandwich panels as stabilizing members, a considerable amount of savings of steel can be achieved for structural members at ambient temperature. These stabilizing effects help to achieve similar savings in case of fire.Originality/valueThis paper summarized briefly the experimental results, numerical simulations and analytical models on the shear behaviour of sandwich panel joints at ambient and elevated temperatures, which were not published yet.