bluuebunny/crossref_metadata_2025_split huggingface dataset:
PSVI-13 Amino Acid Supplementation During the Adaptation Period Did Not Affect the Standardized Ileal Digestibility of Amino Acids in Corn and Soybean Meal Fed to Pigs
Authors: Choi Hyunjun , You Sun Jong , Kim Beob Gyun G 
Published: May 2021

Abstract
The objective was to determine the influence of amino acid (AA) supplementation during the adaptation period on the ileal digestibility of crude protein and AA in corn and soybean meal (SBM). Six barrows with an initial body weight of 30.9 ± 2.6 kg fitted with a T-cannula in the distal ileum were assigned to a 6 × 6 Latin square design with 6 dietary treatments and 6 periods. Two experimental diets contained corn or SBM as the sole source of AA and an N-free diet was additionally prepared. For AA supplementation groups, an AA mixture consisted of Gly, Lys, Met, Thr, Trp, Ile, Val, His, and Phe was added to the corn diet and the N-free diet at the expense of cornstarch, and an AA mixture of Lys, Met, and Thr was added to the SBM diet. All diets contained 0.5% of chromic oxide. The 6 experimental diets were fed to the pigs for 4 and half days, and the 3 diets containing AA mixture were switched to the respective diets without AA mixture during the following 2 and half days. Ileal digesta were collected during the last 2 days. The addition of AA mixture during the adaptation period caused increased apparent ileal digestibility of Arg and Trp in corn (P &lt; 0.05), but did not affect that in SBM. The addition of AA mixture during the adaptation period caused increased apparent ileal digestibility of Pro and Gly regardless of feed ingredient (P &lt; 0.05), but did not affect that of other AA. All AA except Pro in corn and SBM were unaffected by the addition of AA mixture during the adaptation period. In conclusion, the addition of amino acid during the adaptation period does not affect the standardized ileal digestibility of indispensable amino acids in feed ingredients.
PSVI-12 Full-fat Rice Bran as an Alternative Feed Ingredient for Corn in Grow-finish Pigs
Authors: Jayaraman Balachandar , Van Kinh La , Van Sy Phan , González-Vega Caroline , Krishnan Pradeep , Channarayapatna Girish 
Published: May 2021

Abstract
A study was conducted to evaluate the effects of replacing corn by full-fat rice bran (FFRB) on performance and meat quality characteristics of growing-finishing pigs. A total of 728 pigs [Duroc x (Yorkshire x Landrace); initial body weight (BW) = 24.1 ± 0.11 kg] were randomly distributed to 4 dietary treatments with 7 replicate pens (26 pigs/pen) per treatment. Diets 1, 2, 3 and 4 were formulated replacing corn by 5%, 15%, 30%, and 45% of FFRB, respectively. Growth phases include, phase 1 (24 - 60 kg BW), and phase 2 (61 - 105 kg BW). Data were analyzed as a completely randomized design, and each pen was considered as an experimental unit. During phase 1 and 2, average daily gain (ADG) did not differ (P &gt;0.05) when FFRB was increased from 5 to 30% in the diets. Pigs fed 45% FFRB had lower (P&lt; 0.05) ADG in phase 1 and higher in phase 2 than other dietary treatments. Throughout the study, feed intake was not affected (P &gt; 0.05) by dietary treatments. During phase 1, feed conversion ratio (FCR) was similar (P &gt;0.05) among the treatments, however, during phase 2, pigs fed increasing levels of FFRB linearly improved (P&lt; 0.05) FCR. The overall growth performance was similar (P &gt;0.05) among the dietary treatments. Back-fat thickness and meat color were not affected (P &gt;0.05) by treatments. Saturated fatty acids (palmitic acid, stearic acid) in pig muscle (Longissimus) significantly decreased (linear; P&lt; 0.05) with increasing levels of FFRB in pig diets. Pigs fed increasing levels of FFRB had increased linoleic acid (linear; P&lt; 0.05) and linolenic acid (quadratic; P&lt; 0.05) in meat. In conclusion, FFRB could be used as an alternative feed ingredient for corn up to 45% in swine diets without compromising overall growth performance, back-fat thickness and favoring improved unsaturated to saturated fatty acid profile.
PSV-4 Determination of Deoxynivalenol (DON) Content in Biological Samples as an Indicator of DON Intake in Grower-finisher Pigs
Authors: Wellington Michael O , Bosompem Michael A , Nagl Veronika , Columbus Daniel A 
Published: May 2021

Abstract
Due to difficulties in obtaining consistent and/or reliable measures of deoxynivalenol (DON) in complete swine diets, we investigated whether measuring DON in biological samples could be used as an indicator of DON ingestion in pigs. In this study, graded levels of DON (1, 3, or 5 ppm) were fed to grower-finisher pigs for a period of 77-d. On d 35 and 77 of the study, urine samples were quantitatively collected over a 24-h period and blood samples were collected between 3 – 4 h after the morning meal on each of those days for serum DON analysis. For direct quantification of DON in urine, high-performance liquid chromatography with tandem mass spectrometry was performed. For serum samples, indirect quantification of DON was performed via enzymatic hydrolysis. We observed that DON content in urine increased linearly as intake of DON increased (Fig.1A; P &lt; 0.05). Analysis of DON in serum follow a similar trend, where serum DON content was increased as DON intake increased (Fig.1B; P &lt; 0.05). An average of 30% of DON ingested was recovered as DON in urine over a 24-h period. In summary, there was a linear relationship between DON intake and DON content in both urine and blood serum, therefore, analyzing DON concentration in serum and urine could be used as a tool to estimate for DON exposure in pigs under controlled conditions.
PSV-1 Effects of Induction on Piglet Blood Parameters at Time of Farrowing
Authors: Mills Kayla M , Shirley Larissa K , Sharp Katharine G , Garcia Ricardo M , Stewart Kara R 
Published: May 2021

Abstract
Typically, sows are induced to farrow using prostaglandin followed by an injection of oxytocin 24 hours later. Benefits of induction can include decreased rate of stillbirths, dystocia, and postnatal mortality along with increasing the likelihood of farrowings being attended. Several studies have indicated that oxytocin administration may negatively impact fetal oxygen supply during parturition, potentially from umbilical cords breaking prior to birth, resulting in increased preweaning mortality. Therefore, the objective of this study was to determine if various induction protocols impact umbilical cord breakage and fetal blood parameters at birth. Fifty-eight primiparous and multiparous sows were assigned to one of three treatments: no induction (NO; n=24), or 2 cc Lutalyse administered on d114 of gestation followed by either 1 cc of oxytocin 24 hours later (OXY24; n=13) or 0.5 cc of oxytocin at 6 and 12 hours after Lutalyse (OXY6; n=21). Details of the farrowing process were recorded, and umbilical cord blood was collected from piglets at birth and evaluated on an iSTAT machine using an Abbott EC8+ test cartridge. There were no differences in total born, number born alive, stillborns, mummies, or assistance needed during farrowing. Sows in the OXY24 treatment tended to have longer farrowings when compared to both NO and OXY6 (5.6 vs 3.7 vs 3.7 hours; P=0.09). OXY24 gilts (38%) and NO sows (33%) tended to have more piglets born with broken umbilical cords than other parities and treatments (OXY24 sows: 19%; NO gilts: 18%; OXY6 gilts: 25%; OXY6 sows: 18%; P=0.07). Piglets born from NO sows had higher base excess, total carbon dioxide, and glucose which suggests that these piglets had prolonged moments of asphyxiation (P&lt; 0.01). OXY24 piglets had the lowest blood pH which is indicative of hypoxic birthing conditions (P&lt; 0.01). There were no signs of asphyxia in the blood parameters of piglets born from OXY6 sows. Therefore, multiple low doses of oxytocin to induce farrowing may be more beneficial for the welfare of the piglet during farrowing.
PSV-11 Efficacy of Quisqualic Acid as an Amino Acid Transporter Inhibitor in Pig Oocytes
Authors: Reynolds Skyla , Arena Haley A , Sprungl Kimberly , Whitaker Brian D 
Published: May 2021

Abstract
Quisqualic acid is a known inhibitor of sodium-dependent amino acid transporters. However, it is unknown if quisqualic acid has similar effects in in vitro mature oocytes. Therefore, the objective of this study was to determine the optimal dose and effects of quisqualic acid supplemented during maturation. Oocytes (n=362) were supplemented during maturation with quisqualic acid (0, 0.5, 0.75, 1.0, 2.5 mM) to determine the minimum concentration of quisqualic acid that had no effect on oocyte maturation but significantly decreased the intracellular glutathione concentration. The addition of 1.0 mM quisqualic acid was the lowest concentration observed to cause intracellular glutathione levels to be significantly less (P &lt; 0.05) without affecting maturation compared to no quisqualic acid. Based on those results, oocytes were supplemented with or without 1.0 mM quisqualic acid then evaluated for cumulus cell expansion (n=410) and stage of meiosis (n=380) at the end of maturation. Additional oocytes were fertilized and assessed for cortical granule exocytosis (n=400) and kinetics at 12 h after IVF (n=420). Supplementing quisqualic acid to the media did not have an effect on stage of meiosis, fertilization, polyspermic penetration, or cortical granule exocytosis. Supplementing 1.0 mM quisqualic acid significantly decreased (P &lt; 0.05) cumulus cell expansion by the end of maturation and male pronuclear formation by 12 h after IVF. These results suggest that quisqualic acid supplementation during maturation in pigs inhibits sodium-dependent amino acid transporters.
PSIV-6 Effect of a Direct Fed Microbial Feed Additive on Finishing Beef Cattle Performance and Liver Abscess Rate
Authors: Adair Kelton , Fernando Samodha , Erickson Galen E , Bartenslager Alison , Carlson Zachary E 
Published: May 2021

Abstract
A feedlot study was conducted comparing the effects of a direct-fed microbial feed additive (DFM) to no feed additive (CON) on performance and liver abscess rates in finishing beef cattle. The study utilized 60 crossbred steers (initial BW 274 kg ± 2.23) individually fed using a Calan gate system. Steers were housed in separate pens by treatment to avoid DFM cross-contamination, with pen (barn of 30 steers) assigned randomly to each treatment. Cattle were fed a diet consisting of 15% corn silage, 36.5% high moisture corn, 24.5% dry rolled corn, 20% modified distillers grains, and 4% supplement for 189 days. The DFM counts were estimated using cell cytometry and was top dressed at a concentration of approximately 81 billion bacterial cells/head/day. The DFM additive used in this study was developed to reduce the abundance of Fusobacterium necrophorum and Streptococcus bovis in the rumen. No effect of treatment on hot carcass weight (HCW), average daily gain (ADG), dry matter intake (DMI), feed efficiency (G:F), or carcass traits (Table 1) were observed. No significant difference in the occurrence of liver abscesses between treatment groups were observed with 4 steers having abscessed livers in the CON group and 3 steers in the DFM group. Additionally, there were no differences in the severity of liver abscesses; all observed liver abscesses received the score of A. The DFM utilized in this study did not significantly affect performance, liver abscess rate, or the severity of liver abscesses in finishing beef cattle.
PSIV-1 Effect of Corn Silage Inclusion Rate on Apparent Total Tract Digestibility of Dry Matter, Organic Matter, and Crude Protein in Finishing Steers
Authors: Buckhaus Elizabeth M , Smith Zachary K 
Published: May 2021

Abstract
Maine-Anjou × Angus beef steers (n = 156; initial BW 366 ± 37.2 kg) were used in a finishing experiment at the Ruminant Nutrition Center in Brookings, SD. Steers were weighed on 2 consecutive days and assigned into 5 weight blocks. Treatments were arranged in a 2 × 2 factorial, 2 types of implants and 2 dietary treatments with 5 replicate pens for each simple effect. Dietary treatments consisted (DM basis) of 1) 15% (CS15) or 2) 30% corn silage (CS30). Other ingredients consisted of a 50:50 blend of DRC and HMC, liquid supplement and a dry supplement. Bunks were managed using a slick bunk approach and all diets contained (DM basis) 33 mg/kg monensin sodium. Feed collections occurred during the morning and afternoon feedings for two days prior to fecal collections. Each feed sample was composited in equal amounts to create a single sample for each pen. Two fecal collections from each steer occurred 7 hours apart on day 112 of the study. Feces from each steer were composited in equal amounts to create a pen sample. All samples were dried and ground through a 1-mm screen. Acid insoluble ash was used as an internal marker. Apparent total tract digestibility was calculated using the equation: 100-100 ˣ (feed marker/fecal marker) × (fecal variable/feed variable). No interaction between implant and diet was detected for any variables (P ≥ 0.08). One pen was removed from the statistical analysis due to all values being 3 standard deviations away from the mean. Intake did not differ between CS15 and CS30 (P = 0.41). Fecal output was increased 36.9% (P = 0.01) in CS30. Dry matter, organic matter, and crude protein digestion were decreased by feeding CS30 (11.47%, 10.83%, 16.35% respectively; P ≤ 0.03). As corn silage inclusion increases, digestibility coefficients for DM, OM, and CP are decreased.
PSVI-3 Gestational Inulin Supplementation Stabilizes Glucose Metabolism and Improves Reproductive Performance of Sows
Authors: Gao Xin , Hu Qiong , Chan Lawrence , Ma Cally , Zhao Junmei 
Published: May 2021

Abstract
The beneficial effects of dietary fiber on sows performance have been widely studied, but it’s still unclear how fermentable fiber affects sows glucose metabolism and reproduction. The present study aimed to investigate the effects of late gestational inulin supplementation on sows pre-farrowing glucose metabolism and reproductive performance. A total of 106 sows were allotted randomly to 2 groups, receiving either a control diet with 4% wheat flour, or a diet with 4% inulin, from gestation day 85 to 110, both diets met nutrient requirements expressed by NRC. On day 111, sows blood glucose was tested before a common lactation diet meal, then subsequent glucose levels were measured at 1, 2 and 3 hour after meal. Sows were weighed on initial, entry and weaning days. The numbers of live-born and stillborn piglets as well as mortality were recorded. Inulin supplementation didn’t affect sows average daily intake during lactation, and also no significant difference was observed in weaning bodyweight and backfat thickness between two groups. However, the relative wean to entry BW loss was significantly lower in inulin fed sows than control (11.3% vs 13.5%) (P&lt; 0.05). Moreover, postprandial glucose response was less steep in inulin group compared with control, especially glucose concentration at 1h after meal was 10.2% higher in the control group sows. Although the sows farrowing duration was similar in two groups, inulin significantly increased (P&lt; 0.05) proportion of born alive from 90.1% to 93.0% and reduced (P=0.10) still born from 7.3% to 5.4%. Post-foster mortality and livability did not differ between treatments, litter birth weight and average birth weight were not different either. In conclusion, Sows fed 4% inulin diet at late gestation tended to have lower weight loss, slower rate of postprandial blood glucose response and higher born alive and lower stillborn.
PSVI-1 Effect of Light Regiment on Farrowing Performance in Sows
Authors: McLoda Shelby A , Anderson Nichole C , Lugar Drew W 
Published: May 2021

Abstract
The purpose of this study was to investigate the effects of light on farrowing performance in sows. Thirty sows were moved to the farrowing unit at d 110 of gestation and were assigned one of two treatments: 12 h light/dark cycle (Dark) or 24 h light (Light). Treatments were initiated upon entry into the farrowing unit until completion of farrowing was confirmed. For Dark sows, the farrowing crates were structured with light blocking tarps that were lowered at 1900 hr and raised at 0700 hr daily. Video recording devices were installed above all sows and continuous video was recorded from initiation of the treatments until farrowing was complete. Data collected included duration of farrowing, birthing interval, number of stillborn piglets as an indirect measure of dystocia, total born, and liveborn piglets. All statistical analyses were performed using the mixed procedure of SAS, where the experimental unit was individual sow. Funding for this project was provided by the National Pork Checkoff and the U.S. Pork Center of Excellence. Dark sows were more likely to give birth closer to their due date compared to Light sows (1.34 vs. 2.16 ± 0.24 days relative to a 115-day due date, respectively; P = 0.02). Total duration of parturition did not differ between treatments (P = 0.56). Number of liveborn piglets between treatments did not differ (P = 0.68), however the number of stillborn piglets between treatments was different (P = 0.02), where Dark sows had a higher incidence of stillborn piglets compared to Light sows (1.52 vs. 0.65 ± 0.25 stillborn piglets/litter, respectively). The interval between piglets did not differ between treatments (P = 0.93). It is hypothesized that the effects on due date and increased incidence of stillborn piglets was caused by increased stress due to visual isolation in the Dark sows.
PSVI-2 Rising 10-year Trend in Piglet Pre-weaning Mortality in Breeding Herds Associated with Sow Herd Size and Number of Piglets Born Alive
Authors: Koketsu Yuzo 
Published: May 2021

Abstract
The objectives of the present study were to explore the trend in preweaning mortality (PWM) and related measurements such as piglets born alive (PBA), stillborn piglets, herd productivity and herd size in sow herds over a 10-year period; and 2) to examine the relationships between PWM and the related measurements. Herd-level annual data from 2007–2016 for 91 herds in Spain were abstracted from a sow database compiled by a veterinary consultancy firm. The database software automatically calculated herd-level PWM (%) as follows: the total number of piglets born alive to a sow completely weaned during a year (TPBA) minus the total number of piglets weaned by the completely weaned sow during the year divided by TPBA x 100. All the statistical analyses were performed using SAS University Edition. A growth curve model was applied to incorporate correlations for all of the observations arising from the same farm. Herd means of PWM (SD) increased from 11.9 (4.1) % to 14.4 (3.2) %, and mean PBA increased by 1.9 pigs. Mean age of piglet death during lactation increased by 3.8 days, and there was a significant effect of increased year on herd size and the number of piglets weaned per sow per year (PSY; P &lt; 0.05). Higher PWM was associated with more PBA, more stillborn piglets and small herds (lower than the median size: &lt; 570 sows; P &lt; 0.05). Also, as PBA increased from 9 to 14 pigs, PWM in large herds (&gt; 570 sows) increased by only 6.6%, compared with 9.6% in small herds. Furthermore, as PWM decreased from 18 to 8%, herd productivity measured as PSY increased by 2.2 pigs in large herds, but only by 0.6 pigs in small herds. In conclusion, the effect of increased PBA on PWM was alleviated more in large herds than in small herds. Also, the impact of decreased PWM on herd productivity was greater in large herds than in small herds.
PSI-8 Evaluating the Effects of Individual Eating Rates of Feedlot Heifers on Ruminal Fermentation Using an Ex vivo Model
Authors: Zinke Maya , Huizenga Kaylie , Tondini Sara , Shike Daniel W , McCann Joshua C 
Published: May 2021

Abstract
The objective was to determine if the eating rate of individual feedlot heifers affects ruminal fermentation using an ex vivo model. Differences in eating rate could affect ruminal fermentation and predispose an animal to be susceptible to metabolic disorders. From a contemporary group of Simmental × Angus heifers (n = 90; BW = 323 kg), 14 heifers were selected for divergent individual eating rates. During the initial 30 d on the finishing diet, a GrowSafe feed intake system was used to identify the heifers with the 7 fastest (7.60 kg DM / h; 9.25 kg DMI in 1.2 h) and 7 slowest (1.38 kg DM / h; 9.24 kg DMI in 6.7 h) eating rates. Prior to feeding, rumen fluid was collected via esophageal tubing 1 d after intake evaluation period. Each rumen fluid sample was mixed with McDougall’s buffer and a common feed substrate (similar to finishing diet). Then the samples were incubated for 24 and 48 h. In vitro dry matter disappearance (IVDMD), pH, volatile fatty acids (VFA), and ammonia were measured following incubation. The MIXED procedure of SAS 9.4 was used for statistical analysis. No eating rate by incubation time interactions (P ≥ 0.11) were observed. Although heifer eating rate did not affect (P = 0.65) IVDMD, values were greater (P &lt; 0.01) at 48 h compared with 24 h. While time affected pH as it decreased (P &lt; 0.01) from 24 to 48 h, heifer eating rate did not affect (P = 0.84) pH. Eating rate had no effect (P ≥ 0.10) on ammonia, total VFA, and molar proportions of acetate, propionate, isobutyrate, butyrate, valerate, and isovalerate at 24 h. Overall, the results indicated individual eating rates of feedlot heifers did not affect ruminal fermentation using an ex vivo model.
PSI-4 Quantification of Cooling Effects on Basic Tissue Measurements and Exposed Cross-sectional Brain Area in Cadaver Heads from Market Pigs
Authors: Bishop Katherine , Anderson Karly N , Albers Sarah , Allen Kaysie , Huber Christina , Kirk Ashlynn , Olsen Hannah , Vogel Kurt D 
Published: May 2021

Abstract
The objective of this project was to determine the impact of cooling on the soft tissue thickness, cranial thickness, and cross-sectional brain area of cadaver heads from market pigs. Documenting the effect of cooling on tissue dimensions of swine heads is valuable and important for future investigations of physical stunning and euthanasia methods that use cadaver heads. Cadaver heads were sourced from market pigs stunned with CO2. After transport to the data collection location, a captive bolt shot was applied in the frontal position. Following captive bolt application, each head (n=36) underwent an UNCHILLED and CHILLED treatment. The UNCHILLED treatment involved images collected immediately after splitting the head along the bolt path and the CHILLED treatment involved images of the same heads after storage in a walk-in cooler for 24 h at 2–4°C. All measurements for each treatment were determined from images of the heads cut along the plane of the bolt path immediately prior to and immediately after the refrigeration treatment. Measurements were performed by two observers; across all measurements, mean interobserver percent coefficient of variation (%CV) was 11.3±0.6%. The soft tissue posterior to the bolt path was different (P=0.0120) between treatments (CHILLED: 6.4±0.2mm; UNCHILLED: 7.2±0.2mm). The soft tissue thickness anterior to the bolt path was different (P=0.0378) between treatments (CHILLED: 5.5±0.2mm; UNCHILLED: 6.1±0.2mm). There was not a significant difference (P=0.8659) in cranial thickness posterior to the bolt path (CHILLED: 18.1±0.6mm; UNCHILLED: 18.3±0.6mm), nor was there a significant difference (P=0.2593) in cranial thickness anterior to the bolt path between treatments (CHILLED: 16.2±0.6mm; UNCHILLED: 15.2±0.6mm). Cross-sectional brain area did not differ (P=0.0737) (CHILLED: 3633.4±44.1mm; UNCHILLED: 3519.9±44.1mm). A correction factor of 1.12 was determined from this study for cases where estimation of UNCHILLED soft tissue thickness from CHILLED soft tissue thickness is necessary.