bluuebunny/crossref_metadata_2025_split huggingface dataset:
Ground-dwelling spider families and forest structure variables for monitoring ecologically sustainable logging operations
Authors: Alcalde Ana Sofía , Politi Natalia , Rodríguez-Artigas Sandra , Corronca José Antonio , Rivera Luis Osvaldo 
Published: July 2021

SummaryApproximately 80% of neotropical forests are subject to unsustainable economic practices, such as logging. Spiders are a megadiverse taxonomic group with a particularly great diversity in forest ecosystems and could help indicate the sustainability of logging operations. At six sites at 400–700 m altitude in the piedmont forest of north-western Argentina, spiders collected using pitfall traps and forest structure and spider assemblage structure variables were quantified in order to examine the association between them and to identify indicator spider families. Logging changes forest structure and seems to generate an unsuitable habitat for spiders associated with mature forests. The family taxonomic level is a good surrogate for spider morphospecies. The Mysmenidae, Nemesiidae, Theridiidae, Pholcidae, Hahniidae and Tetragnathidae families were associated with upper canopy cover of 20% or more and with more than two dead fallen trees per 0.1 ha and >15 live trees per 0.1 ha, found in unlogged forests. Bearing in mind that the piedmont forest of north-western Argentina is being logged in the absence of sustainability criteria, we suggest including spiders in monitoring schemes to complement the information obtained from more readily used groups, such as charismatic vertebrates.
An investigation of machine translation output quality and the influencing factors of source texts
Authors: Lee Sangmin-Michelle 
Published: July 2021

Abstract
The use of machine translation (MT) in the academic context has increased in recent years. Hence, language teachers have found it difficult to ignore MT, which has led to some concerns. Among the concerns, its accuracy has become a major factor that shapes language teachers’ pedagogical decision to use MT in their language classrooms. Despite the urgency of the issue, studies on MT output quality in foreign language education remain scarce. Moreover, as MT is advancing every year, updated studies are imperative. Therefore, the present study investigated the quality of MT outputs (Google Translate) from Korean to English by comparing it with the English-translated texts of intermediate English as a foreign language students. The study also examined the factors within the source texts that affect the quality of MT outputs. Five trained evaluators examined multiple aspects of MT output samples (N = 104) and students’ English texts (N = 104), including mechanics, vocabulary, grammar, and context. The results showed that both texts were equally comprehensible, but MT outperformed the students in most aspects under investigation. The study further found that only two factors in the source texts – punctuation and sentence complexity – influenced MT output quality, whereas lexical and grammatical accuracy, lexical diversity, and contextual understanding did not affect it. Based on the results, the study presents classroom implications for using MT for educational purposes.
Evaluation of primary care services in Hungary: a comprehensive description of provision, professional competences, cooperation, financing, and infrastructure, based on the findings of the Hungarian-arm of the QUALICOPC study
Authors: Rurik Imre , Nánási Anna , Jancsó Zoltán , Kalabay László , Lánczi Levente István , Móczár Csaba , Semanova Csilla , Schmidt Péter , Torzsa Péter , Ungvári Tímea , Kolozsvári László Róbert 
Published: July 2021

Abstract

Background:
Primary health care provision in terms of quality, equity, and costs are different by countries. The Quality and Costs of Primary Care (QUALICOPC) study evaluated these domains and parameters in 35 countries, using uniformized method with validated questionnaires filled out by family physicians/general practitioners (GPs).
This paper aims to provide data of the Hungarian-arm of the QUALICOPC study and to give an overview about the recent Hungarian primary care (PC) system.

Methods:
The questionnaires were completed in 222 Hungarian GP practices, delivered by fieldworkers, in a geographically representative distribution. Descriptive analysis was performed on the data.

Findings:
Financing is based mostly on capitation, with additional compensatory elements and minor financial incentives. The gate-keeping function is weak. The communication between GPs and specialists is often insufficient. The number of available devices and equipment are appropriate. Single-handed practices are predominant. Appointment instead of queuing is a new option and is becoming more popular, mainly among better-educated and urban patients. GPs are involved in the management of almost all chronic condition of all generations. Despite the burden of administrative tasks, half of the GPs estimate their job as still interesting, burn-out symptoms were rarely found. Among the evaluated process indicators, access, continuity, comprehensiveness, and coordination were rated as satisfactory, together with equity among health outcome indicators. Financing is insufficient; therefore, many GPs are involved in additional income-generating activities. The old age of the GPs and the lack of the younger GPs generation contributes to a shortage in manpower. Cooperation and communication between different levels of health care provision should be improved, focusing better on community orientation and on preventive services. Financing needs continuous improvement and appropriate incentives should be implemented. There is a need for specific PC-oriented guidelines to define properly the tasks and competences of GPs.
Quasi‐Stationary Intense Rainstorms Spread Across Europe Under Climate Change
Authors: Kahraman Abdullah , Kendon Elizabeth J. , Chan Steven C. , Fowler Hayley J. 
Published: July 2021

AbstractUnder climate change, increases in precipitation extremes are expected due to higher atmospheric moisture. However, the total precipitation in an event also depends on the condensation rate, precipitation efficiency, and duration. Here, a new approach following an “ingredients‐based methodology” from severe weather forecasting identifies important aspects of the heavy precipitation response to climate change, relevant from an impacts perspective and hitherto largely neglected. Using 2.2 km climate simulations, we show that a future increase in precipitation extremes across Europe occurs, not only because of higher moisture and updraft velocities, but also due to slower storm movement, increasing local duration. Environments with extreme precipitation potential are 7× more frequent than today by 2100, while the figure for quasi‐stationary ones is 11× (14× for land). We find that a future reduction in storm speeds, possibly through Arctic Amplification, could enhance event accumulations and flood risk beyond expectations from studies focusing on precipitation rates.
How Does LCL Height Influence Deep Convective Updraft Width?
Authors: Mulholland J. P. , Peters J. M. , Morrison H. 
Published: July 2021

AbstractPrevious studies have hypothesized that the width of deep convection should positively scale with lifting condensation level (LCL) height. To evaluate this hypothesis, we analyzed idealized large‐eddy simulations with varying LCL heights and initial warm bubble widths in unsheared environments with comparable convective available potential energy. For a given initial warm bubble width, simulations with higher LCLs result in wider, deeper, and stronger cloudy updrafts compared to simulations with lower LCLs. Rising dry thermals in higher LCL simulations experience longer residence times within the sub‐cloud layer, and consequently entrain more conditionally unstable air and grow wider before reaching the LCL. The resulting cloudy updrafts are wider, deeper, and have faster vertical velocities because of a reduction in entrainment‐driven dilution of buoyancy, relative to lower LCL simulations. These results confirm the hypothesized positive relationship between LCL height and deep convective updraft width, and provide a physical explanation for this relationship.
Uncertainty of Tropical Cyclone Wind Radii on Sea Surface Temperature Cooling
Authors: Pun Iam‐Fei , Knaff John A. , Sampson Charles R. 
Published: July 2021

AbstractThe sea surface temperature (SST) beneath a tropical cyclone (TC) is of great importance to its dynamics; therefore, understanding and accurately estimating the magnitude of SST cooling is of vital importance. Existing studies have explored important influences on SST of TC translation speed, maximum surface winds, ocean thermal condition, and ocean stratification. But the influence of the TC wind radii (or collectively called the TC size) on SST has been largely overlooked. In this study, we assess the influence of wind radii uncertainty on SST cooling by a total of 15,983 numerical simulations for the western North Pacific during the 2014–2018 seasons. Results show a 6%–20% SST cooling error induced using wind radii from the Joint Typhoon Warning Center official forecast and a 35%–40% SST cooling error using wind radii from the operational runs of the Hurricane Weather Research and Forecasting (HWRF) model. Our results indicate that SST cooling is most sensitive to the radius of 64 kt winds (R64) due to its effects on the integrated kinetic energy of the TC and subsequent mixing of the ocean surface layer. It is also found that the correlation between SST cooling induced by the TC and its size is 0.49, which is the highest among all the parameters tested. This suggests that it is extremely important to get TC size correct in order to predict the SST cooling response, which then impacts TC evolution in numerical weather prediction models.
DNA repair- and nucleotide metabolism-related genes exhibit differential CHG methylation patterns in natural and synthetic polyploids (Brassica napus L.)
Authors: Yin Liqin , Zhu Zhendong , Huang Liangjun , Luo Xuan , Li Yun , Xiao Chaowen , Yang Jin , Wang Jisheng , Zou Qiong , Tao Lanrong , Kang Zeming , Tang Rong , Wang Maolin , Fu Shaohong 
Published: July 2021

AbstractPolyploidization plays a crucial role in the evolution of angiosperm species. Almost all newly formed polyploids encounter genetic or epigenetic instabilities. However, the molecular mechanisms contributing to genomic instability in synthetic polyploids have not been clearly elucidated. Here, we performed a comprehensive transcriptomic and methylomic analysis of natural and synthetic polyploid rapeseeds (Brassica napus). Our results showed that the CHG methylation levels of synthetic rapeseed in different genomic contexts (genes, transposon regions, and repeat regions) were significantly lower than those of natural rapeseed. The total number and length of CHG-DMRs between natural and synthetic polyploids were much greater than those of CG-DMRs and CHH-DMRs, and the genes overlapping with these CHG-DMRs were significantly enriched in DNA damage repair and nucleotide metabolism pathways. These results indicated that CHG methylation may be more sensitive than CG and CHH methylation in regulating the stability of the polyploid genome ofB. napus. In addition, many genes involved in DNA damage repair, nucleotide metabolism, and cell cycle control were significantly differentially expressed between natural and synthetic rapeseeds. Our results highlight that the genes related to DNA repair and nucleotide metabolism display differential CHG methylation patterns between natural and synthetic polyploids and reveal the potential connection between the genomic instability of polyploid plants with DNA methylation defects and dysregulation of the DNA repair system. In addition, it was found that the maintenance of CHG methylation inB. napusmight be partially regulated byMET1. Our study provides novel insights into the establishment and evolution of polyploid plants and offers a potential idea for improving the genomic stability of newly formedBrassicapolyploids.
Conductivity of the phloem in mango (Mangifera indica L.)
Authors: Barceló-Anguiano Miguel , Hormaza José I. , Losada Juan M. 
Published: July 2021

AbstractMango (Mangifera indica L., Anacardiaceae), the fifth most consumed fruit worldwide, is one of the most important fruit crops in tropical regions, but its vascular anatomy is quite unexplored. Previous studies examined the xylem structure in the stems of mango, but the anatomy of the phloem has remained elusive, leaving the long-distance transport of photoassimilates understudied. We combined fluorescence and electron microscopy to evaluate the structure of the phloem tissue in the tapering branches of mango trees, and used this information to describe the hydraulic conductivity of its sieve tube elements following current models of fluid transport in trees. We revealed that the anatomy of the phloem changes from current year branches, where it was protected by pericyclic fibres, to older ones, where the lack of fibres was concomitant with laticiferous canals embedded in the phloem tissue. Callose was present in the sieve plates, but also in the walls of the phloem sieve cells, making them discernible from other phloem cells. A scaling geometry of the sieve tube elements—including the number of sieve areas and the pore size across tapering branches—resulted in an exponential conductivity towards the base of the tree. These evaluations in mango fit with previous measurements of the phloem architecture in the stems of forest trees, suggesting that, despite agronomic management, the phloem sieve cells scale with the tapering branches. The pipe model theory applied to the continuous tubing system of the phloem appears as a good approach to understand the hydraulic transport of photoassimilates in fruit trees.
VqMYB154 promotes polygene expression and enhances resistance to pathogens in Chinese wild grapevine
Authors: Jiang Changyue , Wang Dan , Zhang Jie , Xu Yan , Zhang Chaohong , Zhang Jianxia , Wang Xiping , Wang Yuejin 
Published: July 2021

AbstractResveratrol plays a crucial phytoalexin role in the grapevine and is beneficial to human health. However, the molecular mechanism of resveratrol accumulation in the enhancement of disease resistance is unclear. Here, we report that the transcription factor VqMYB154 from Vitis quinquangularis accession Danfeng-2 is strongly expressed under artificial inoculation with Uncinula necator and regulates resveratrol accumulation. Unlike its homolog, VqMYB154 has a pathogen-induced promoter and responds to stimulation by U. necator, Pseudomonas syringae, and other treatments. Yeast one-hybrid and GUS activity assays confirmed that VqMYB154 can activate the stilbene synthase genes VqSTS9, VqSTS32, and VqSTS42 by directly binding to their promoters. Overexpression of VqMYB154 in grape leaves resulted in activation of the stilbene pathway, upregulation of STS genes, and accumulation of stilbenoids. In addition, heterologous overexpression of VqMYB154 in Arabidopsis activated resistance-related genes and resulted in greater programmed cell death and accumulation of reactive oxygen species, which led to resistance against P. syringae. These results suggest that the transcription factor VqMYB154 from V. quinquangularis accession Danfeng-2 participates in the regulatory mechanism that improves the biosynthesis and accumulation of stilbenes and enhances resistance to disease in grapevine.
Evolution of the MLO gene families in octoploid strawberry (Fragaria ×ananassa) and progenitor diploid species identified potential genes for strawberry powdery mildew resistance
Authors: Tapia Ronald R , Barbey Christopher R , Chandra Saket , Folta Kevin M , Whitaker Vance M , Lee Seonghee 
Published: July 2021

Abstract
Powdery mildew (PM) caused by Podosphaera aphanis is a major fungal disease of cultivated strawberry. Mildew Resistance Locus O (MLO) is a gene family described for having conserved seven-transmembrane domains. Induced loss-of-function in specific MLO genes can confer durable and broad resistance against PM pathogens. However, the genomic structure and potential role of MLO genes for PM resistance have not been characterized yet in the octoploid cultivated strawberry. In the present study, MLO gene families were characterized in four diploid progenitor species (Fragaria vesca, F. iinumae, F. viridis, and F. nipponica) and octoploid cultivated (Fragaria ×ananassa) strawberry, and potential sources of MLO-mediated susceptibility were identified. Twenty MLO sequences were identified in F. vesca and 68 identified in F. ×ananassa. Phylogenetic analysis divided diploid and octoploid strawberry MLO genes into eight different clades, in which three FveMLO (MLO10, MLO17, and MLO20) and their twelve orthologs of FaMLO were grouped together with functionally characterized MLO genes conferring PM susceptibility. Copy number variations revealed differences in MLO composition among homoeologous chromosomes, supporting the distinct origin of each subgenome during the evolution of octoploid strawberry. Dissecting genomic sequence and structural variations in candidate FaMLO genes revealed their potential role associated with genetic controls and functionality in strawberry against PM pathogen. Furthermore, the gene expression profiling and RNAi silencing of putative FaMLO genes in response to the pathogen indicate the function in PM resistance. These results are a critical first step in understanding the function of strawberry MLO genes and will facilitate further genetic studies of PM resistance in cultivated strawberry.
Antagonism between abscisic acid and gibberellin regulates starch synthesis and corm development in Gladiolus hybridus
Authors: Li Jingru , Seng Shanshan , Li Donglei , Zhang Fengqin , Liu Yixuan , Yao Ting , Liang Jiahui , Yi Mingfang , Wu Jian 
Published: July 2021

AbstractUnderstanding corm development in flower bulbs is of importance for securing the quality of cut flowers and propagation of commercial stocks. Gladiolus is one of the most popular bulb plants worldwide. Its corm development is characterized by starch accumulation. Previous research has shown that phytohormones (especially gibberellin (GA)) are involved in tuber development. However, the relationship between abscisic acid (ABA)/GA and starch during corm development remains unclear. To gain deeper insights into the biological process of corm development, we performed a detailed anatomical characterization of different stages of corm development and analyzed phytohormone levels. Our study showed that corm development is linked to hormones (ABA and GA) and carbohydrates (sucrose and starch). Exogenous hormone treatment and silencing of endogenous hormone biosynthesis genes indicated that ABA positively regulates corm development, while GA acts as an antagonist of ABA function. A sucrose synthase gene (GhSUS2) was shown to be involved in the antagonism between ABA and GA. GhSUS2 was upregulated by ABA and downregulated by GA. The increase in the transcript level of GhSUS2 coincided with the development of corm/cormels. Silencing of GhSUS2 repressed corm development and starch accumulation. In conclusion, we propose that GhSUS2, an essential enzyme in sucrose degradation, is differentially regulated by ABA and GA and controls corm development in Gladiolus.
Evaluation of ambient mass spectrometry tools for assessing inherent postharvest pepper quality
Authors: Mason Tyler J. , Bettenhausen Harmonie M. , Chaparro Jacqueline M. , Uchanski Mark E. , Prenni Jessica E. 
Published: July 2021

AbstractHorticulturists are interested in evaluating how cultivar, environment, or production system inputs can affect postharvest quality. Ambient mass spectrometry approaches enable analysis of minimally processed samples under ambient conditions and offer an attractive high-throughput alternative for assessing quality characteristics in plant products. Here, we evaluate direct analysis in real time (DART-MS) mass spectrometry and rapid evaporative ionization-mass spectrometry (REIMS) to assess quality characteristics in various pepper (Capsicum annuum L.) cultivars. DART-MS exhibited the ability to discriminate between pod colors and pungency based on chemical fingerprints, while REIMS could distinguish pepper market class (e.g., bell, lunchbox, and popper). Furthermore, DART-MS analysis resulted in the putative detection of important bioactive compounds in human diet such as vitamin C, p-coumaric acid, and capsaicin. The results of this study demonstrate the potential for these approaches as accessible and reliable tools for high throughput screening of pepper quality.
Copper(I)-catalysed site-selective C(sp3)–H bond chlorination of ketones, (E)-enones and alkylbenzenes by dichloramine-T
Authors: Jin Jianwen , Zhao Yichao , Kyne Sara Helen , Farshadfar Kaveh , Ariafard Alireza , Chan Philip Wai Hong 
Published: July 2021

AbstractStrategies that enable intermolecular site-selective C–H bond functionalisation of organic molecules provide one of the cornerstones of modern chemical synthesis. In chloroalkane synthesis, such methods for intermolecular site-selective aliphatic C–H bond chlorination have, however, remained conspicuously rare. Here, we present a copper(I)-catalysed synthetic method for the efficient site-selective C(sp3)–H bond chlorination of ketones, (E)-enones and alkylbenzenes by dichloramine-T at room temperature. A key feature of the broad substrate scope is tolerance to unsaturation, which would normally pose an immense challenge in chemoselective aliphatic C–H bond functionalisation. By unlocking dichloramine-T’s potential as a chlorine radical atom source, the product site-selectivities achieved are among the most selective in alkane functionalisation and should find widespread utility in chemical synthesis. This is exemplified by the late-stage site-selective modification of a number of natural products and bioactive compounds, and gram-scale preparation and formal synthesis of two drug molecules.
Fluid flow-induced left-right asymmetric decay of Dand5 mRNA in the mouse embryo requires a Bicc1-Ccr4 RNA degradation complex
Authors: Minegishi Katsura , Rothé Benjamin , Komatsu Kaoru R. , Ono Hiroki , Ikawa Yayoi , Nishimura Hiromi , Katoh Takanobu A. , Kajikawa Eriko , Sai Xiaorei , Miyashita Emi , Takaoka Katsuyoshi , Bando Kana , Kiyonari Hiroshi , Yamamoto Tadashi , Saito Hirohide , Constam Daniel B. , Hamada Hiroshi 
Published: July 2021

AbstractMolecular left-right (L-R) asymmetry is established at the node of the mouse embryo as a result of the sensing of a leftward fluid flow by immotile cilia of perinodal crown cells and the consequent degradation of Dand5 mRNA on the left side. We here examined how the fluid flow induces Dand5 mRNA decay. We found that the first 200 nucleotides in the 3′ untranslated region (3′-UTR) of Dand5 mRNA are necessary and sufficient for the left-sided decay and to mediate the response of a 3′-UTR reporter transgene to Ca2+, the cation channel Pkd2, the RNA-binding protein Bicc1 and their regulation by the flow direction. We show that Bicc1 preferentially recognizes GACR and YGAC sequences, which can explain the specific binding to a conserved GACGUGAC motif located in the proximal Dand5 3′-UTR. The Cnot3 component of the Ccr4-Not deadenylase complex interacts with Bicc1 and is also required for Dand5 mRNA decay at the node. These results suggest that Ca2+ currents induced by leftward fluid flow stimulate Bicc1 and Ccr4-Not to mediate Dand5 mRNA degradation specifically on the left side of the node.