bluuebunny/crossref_metadata_2025_split huggingface dataset:
Laser Damage Threshold of Hydrophobic Up-Conversion Carboxylated Nanocellulose/SrF2:Hо Composite Films Functionalized With 3-Aminopropyltriethoxysilane
Authors: Luginina Anna A. , Kuznetsov Sergey V. , Ivanov Vladimir K. , Voronov Valery V. , Yapryntsev Alexey D. , Lyapin Andrey A. , Chernova Elena V. , Pynenkov Aleksandr A. , Nishchev Konstantin N. , Gainutdinov Radmir V. , Bogach Alexey V. , Fedorov Pavel P. 
Published: May 2021

Abstract
Luminescent nanocomposite films, containing SrF 2 :Но up-conversion particles, were prepared by two different protocols from aqueous dispersions of TEMPO-oxidized cellulose nanofibrils (TOCNF) functionalized with 3-aminopropyltriethoxysilane (APS) without the use of organic solvents at pH = 4.0-4.5 and 9.0-9.5, respectively. Proposed synthetic protocols included formation of the films by drying the dispersions containing pre-hydrolyzed APS adsorbed onto TOCNF and SrF 2 :Но particles followed by heating at 105 °C. Hydrophobic (water contact angle 101 ± 2°), strong, and translucent TOCNF/SrF 2 :Но-APS films were prepared by casting from a solution at pH = 4.0-4.5. Scanning electron microscopy, energy-dispersive X-ray spectroscopy with element mapping, Fourier-transform infrared spectroscopy, X-ray diffraction methods confirmed homogeneous distribution of up-conversion particles in TOCNF matrices as well as the grafting of linear polysiloxanes via the condensation of silanol groups and OH-groups on the surface of TOCNF. Differential scanning calorimetry and thermogravimetry data confirmed an increase in thermal stability of the APS modified nanocomposite films obtained at pH = 4.0-4.5. Hydrophobic TOCNF/SrF 2 :Но-APS nanocomposite films exhibited an intense red luminescence in the visible spectrum range ( 5 I 7 level excitation of Ho 3+ ions with 1912 nm laser irradiation) as well as two-times higher laser damage threshold compared to unmodified TOCNF/SrF 2 :Но films. TOCNF/SrF 2 :Но films can be used for visualization 2 μm laser radiation in medicine and long-distance atmosphere monitoring.
Virtual autopsy and confirmation of normal fetal heart anatomy in the first trimester using three-dimensional (3D) reconstruction of histological sections
Authors: Ruican Dan , Petrescu Ana-Maria , Ungureanu Anda , Pirici Daniel , Marinaș Marius Cristian , Ofiteru Anca Maria , Serbanescu Mircea , Simionescu Cristina , Badiu Anne Marie , Roșu Gabriela-Camelia , Belciug Smaranda , Iliescu Dominic Gabriel 
Published: May 2021

Abstract
Objective: In this pilot study we tested the feasibility of cardiac structures reconstruction from histologic sections in 12-13 weeks normal fetuses. Conventional autopsy is hampered at this gestational age because of the small size of the heart anatomical structures, while alternative non-invasive methods for pathology examination of the fetus are expensive, rarely available and lack accuracy data regarding the confirmation of first trimester heart defects.Method: Normal hearts from fetuses aged 12-13 gestational weeks were removed for histological preparation, virtual reconstruction and cardiac structures analysis. All sections have been scanned and a three-dimensional (3D) reconstruction of the whole organ has been rendered. Results: 5 cases were investigated. Visualization of the normal heart cavities, including atrio-ventricular septum was very good in all fetuses. The entire course of right and left ventricle outflow tracts was confidently confirmed, along the and branching pattern of aorta and pulmonary artery trunk.Conclusion: The use of 3D reconstruction of fetal heart histological sections in first trimester may serve as an important audit to confirm the normalcy of heart structures. The histological and post processed information is retained, and this volume can be stored, reanalyzed or sent online for a second opinion.
Delayed Cutaneous Hypersensitivity Reaction to Vaxzevria (ChAdOx1-S) Vaccine against SARS-CoV-2
Authors: Sprute Rosanne , Schumacher Sofie , Pauls Martina , Pauls Wolfgang , Cornely Oliver A. 
Published: May 2021

Abstract
Recently, an increasing number of cases with delayed large cutaneous reaction after immunisation with mRNA-based vaccines have been reported. This adverse reaction – which is considered a delayed-type or T-cell mediated hypersensitivity reaction – has been described primarily for the Moderna (mRNA-1273) vaccine and to a lesser extent for Comirnaty (Pfizer/BioNTech, BNT162b2).We describe a delayed large cutaneous reaction in a patient who received the viral vector vaccine Vaxzevria (ChAdOx1-S). The time course and clinical symptoms of delayed major skin reaction after vaccination with Moderna or Pfizer/BioNTech have a similar pattern that we recognized in our patient after Vaxzevria vaccination. In contrast to the Moderna vaccine trials, this phenomenon has not been described in the Vaxzevria clinical trials and is to the best of our knowledge the first report of this adverse reaction to a vector-based vaccine against SARS-CoV-2.With this, we hope to raise awareness about delayed injection site reactions that also occur after viral vector vaccines and to encourage additional reporting and patient education regarding the cutaneous reactions after COVID-19 vaccination.
Probiotic feeding effect of Bacillus subtilis on broilers chicks’ microflora, TLRs and interleukin gene expression
Authors: Wang Zaigui , Yang Ru , Khan Salman , wei Chunjie , Qian Hu , Khalid Anam 
Published: May 2021

Abstract
The broiler chicks provide the major portion of daily food items. The current study aimed to investigate the effects of Bacillus subtilis (BS) on broiler chicken gut microflora diversity, digestive enzyme activity and expression of Toll-like receptors (TLRs). A total of 240 crossbred broiler chickens were randomly allocated into 4 groups with 3 replicates. The groups were named as control group (basal diet, BD), group Ⅰ (BD with 300 g/d BS,1.08×107 CFU/kg), group Ⅱ (BD with 600 g/d BS, 2.16×107 CFU/kg), and group Ⅲ (BD with 900 g/d BS, 3.24×107 CFU/kg). Samples were collected at the 21st day. The difference of α diversity and β diversity between control group and treatment groups were not significant, but the abundance of some microorganisms in the treatment groups were improved, and the core microorganisms were different between groups. In comparison of control group, the protease activity in ileum of experimental groups was significantly increased (p < 0.05). Protease activity in ileum of group Ⅱ and Ⅲ was improved by 22.59% (p < 0.01) and 14.49% (p < 0.05). The amylase activity in ileum of treatment groups were also significantly increased (p < 0.05). Amylase activity of group Ⅱ was increased by 41.85% (p < 0.01) in comparison to control, group Ⅰ and Ⅲ. Expression of TLR1A and TLR7 in the jejunum and caecum of treatment groups were significantly up-regulated while their expression in ileum was decreased. Similarly, the expression of TLR1B gene was significantly improved in the ileum, and down regulated in the cecum. Transcription levels of TLR2A and MyD88 in jejunum, liver, spleen and kidney were significantly increased, but their expression was significantly lower in the ileum and cecum. The TLR2B, TLR3, TLR4, TLR15, TLR21 expression were also significantly modulated in various organ of broiler chicken. The most important inflammatory factor such as IL-1β expressions in spleen and kidney were significantly increased in the current data. Bacillus subtilis could not regulate the caecal microflora diversity, but improved amount of some gut probiotics such as lactobacillus, the digestive enzyme activity, regulate some immunogenic expressions and enhance the immune capacity of animal. We hope that the impact of this data will explore the diseases control and feeding quality of broiler chicks at industrial level.
Factors Associated With Adherence to Lockdown Measures in Uganda
Authors: Muzyamba Choolwe , Makova Ogylive , Mushibi Geofrey 
Published: May 2021

Abstract
Background

While the debate regarding the usefulness of the lockdown goes on in research, it has been highlighted that this debate is pointless unless studies demonstrate to what extent people are actually abiding by the lockdown. Studies on factors associated with adherence of COVID-19-related lockdowns have largely been conducted in western countries. However, similar studies are still lacking in low income countries like Uganda. This study fills this gap by investigating factors associated with adherence to lockdown in Uganda.
Methods

A qualitative cross sectional survey was conducted online via the AfriSight platform with a sample size of 1249. These participants were selected randomly from all across Uganda. The data was analyzed descriptively and for inferential statistics we analyzed the data using probit regression. This allowed us to investigate the various motivations, socioeconomic, and demographic factors that help predict adherence or lack thereof to lockdown measures. Based on this probit analysis, we further generated marginal effects to ascertain the probabilities of adhering to lockdown.
Results

Our study reports the complexity that characterizes adherence to the lockdown in Uganda. Various socioeconomic and demographic factors particularly, age, gender, size of household, religion, income level, and employment status all seem to uniquely interact in shaping adherence behavior.
Conclusion

This study demonstrates that any lockdown-related efforts to minimize the spread of COVID-19 must pay attention to how these factors playout in the context of Uganda. Whereas most of the factors observed can more easily be handled in wealthier countries which provide social benefits during lockdowns, Uganda must rethink the uncritical adoption of such measures without localizing them. There is a need for Uganda to invest in a COVID-19 response that is alive to local context and reality, and one which commands wide support and adherence.
Schistosoma Japonicum Peptide SJMHE1 Inhibits Acute And Chronic Colitis Induced By Dextran Sulphate Sodium In Mice
Authors: Shan Wenqi , Zhang Wenzhe , Xue Fei , Ma Yongbin , Dong Liyang , Wang Ting , Zheng Yu , Feng Dingqi , Chang Ming , Yuan Guoyue , Wang Xuefeng 
Published: May 2021

Abstract
Background: Harnessing helminth-based immunoregulation is a novel therapeutic strategy for many immune dysfunction disorders, including inflammatory bowel diseases. We previously identified a small-molecule peptide from Schistosoma japonicum and named it SJMHE1. SJMHE1 can suppress delayed-type hypersensitivity, collagen-induced arthritis, and asthma in mice. In this study, we assessed the effects of SJMHE1 on dextran sulfate sodium (DSS)-induced acute and chronic colitis. Methods: C57BL/6 mice were induced acute and chronic colitis by DSS, and were injected by emulsifier SJMHE1 or PBS. Then the mice were examined body weight loss, disease activity index, colon lengths, histopathology change, cytokine expression and helper T (Th) cell subset distribution.Results: We found that SJMHE1 treatment significantly suppressed DSS-induced acute and chronic colitis, improved disease activity and colon pathological damage. SJMHE1 treatment modulated the expression of pro-inflammatory and anti-inflammatory cytokines in splenocytes and the colon. Furthermore, SJMHE1 treatment reduced the percentage of Th1 and Th17 cells and increased the percentage of Th2 and regulatory T (Treg) cells in the splenocytes and mesenteric lymph nodes (MLNs) from mice with acute colitis. Similarly, SJMHE1 treatment upregulated the expression of IL-10 mRNA, and downregulated the expression of IL-17 mRNA, and modulated the Th cell balance in mice with chronic colitis. Conclusions: Our data show that SJMHE1 provided protection against acute and chronic colitis by restoring immune balance. As a small molecule, SJMHE1 might be a novel agent for the treatment of IBD without immunogenicity concerns.
Overexpression Of RNPEPL1 Predicts Poor Overall Survival In Ovarian Carcinoma
Authors: Chen Junchen , Peng Yanzhen , Cheng Jiumei 
Published: May 2021

Abstract
Background. RNPEPL1 (arginyl aminopeptidase-like 1) belongs to the M1 family of zinc metallopeptidases. We aimed to explore the role of RNPEPL1 in the occurrence and progression of ovarian carcinoma (OV).Methods: We analyzed the expression level and prognostic value of RNPEPL1 in OV across Oncomine, GEO, TCGA and Human Protein Atlas (HPA) database and identified its prognostic value by the Cox regression analysis and Kaplan–Meier method. We utilized GESA software to perform GO and KEGG analyses. We also utilized TIMER to evaluate the relevance between RNPEPL1 and tumor-infiltrating immune cells. Finally, relevance between RNPEPL1 and immune-related genes was examined by Pearson correlation analysis.Results: RNPEPL1 was overexpressed in OV tissues compared with normal ovary tissue. Moreover, high RNPEPL1 expression was related with poor OV survival in TCGA. Cox analyses suggested that overexpression of RNPEPL1 was an independent risk factor for overall survival(OS) of OV. Results of KEGG analysis suggested that genes were mainly enriched in T cell receptor signaling pathway, B cell receptor signaling pathway and natural killer cell mediated cytotoxicity when the expression level of RNPEPL1 is low. Besides, RNPEPL1 expression is associated with infiltrating levels of CD4+ T cells, B cells, macrophage, neutrophils and dendritic cells. And, the level of dendritic cells is correlated with OS of OV. RNPEPL1 expression was significantly correlated with the immune signaling pathway of antigen processing and presentation.Conclusions: RNPEPL1 is a novel independently prognostic biomarker of OV and was related to immune infiltration. Especially, the tight correlation between RNPEPL1 and antigen processing and presentation may be the potential hub of anti-tumor immunity and one of the essential factors influencing survival.
Diazinon Reduction In Apple Juice Using Probiotic Bacteria During Fermentation And Refrigerated Storage
Authors: Rezaei Farahnaz , Nejati Roghayeh , Sayadi Mehran , Nematillahi Amene 
Published: May 2021

Abstract
The main objective of this work was to study the effects of probiotic strains, probiotic primary inoculated population, concentrations of spiked diazinon, physiology of probiotic bacteria, fermentation times and cold storage period in six consecutive stages on diazinon reduction in apple juice. Chemical properties (pH, total acidity, and sugar content), probiotic viability and diazinon reduction percent were monitored during fermentation and cold storage. Dispersive Solid Phase Extraction (dSPE) followed by gas chromatography-mass spectrometry was used to extract and measure diazinon concentration. Results showed that Lactobacillus acidophilus revealed the highest ability to reduce diazinon in apple juice after fermentation. Inoculation of L. acidophilus at 9 log CFU/mL, showed significantly higher diazinon reducing ability than 7 log CFU/mL. Ability of L. acidophilus to reduce the concentration of 1000 µg/L of spiked diazinon was significantly greater than 5000 µg/L. Heat-killed (dead) L. acidophilus bacteria reduced less diazinon content at the end of fermentation than viable bacteria. Furthermore, 72 h of fermentation was more effective in diazinon reduction. Level of diazinon in treatments containing L. acidophilus decreased significantly during cold storage period, so that it is completely disappeared at the end of storage (28 days) along with maintaining health-promoting properties of probiotic apple juice.
Polyphenol-rich extract of Ocimum gratissimum leaves Prevented Toxic Effects of Cyclophosphamide on the kidney Function of Wistar rats
Authors: Alabi Quadri K. , Akomolafe Rufus O. , Akomolafe Joseph G. , Aturamu Ayodeji , Ige Mokolade S. , Kayode Oyindasola O. , Kajewole Deborah I. 
Published: May 2021

Abstract
Background Cyclophosphamide (CP) is one of the potent and low cost chemotherapy used in clinical setting against a variety of tumors. However, its association with nephrotoxicity limits its therapeutic use. Ocimum gratissimum leaf is a natural plant with numerous pharmacological and therapeutic efficacies, such as antioxidant, anti-inflammation, and anti-apoptotic properties.Methods The present study was designed to evaluate the protective effect of Ocimum gratissimum (OG) against CP-induced kidney dysfunction in rats. Rats were pre-treated with 400 mg/kg b.w. of polyphenol-rich Ocimum gratissimum leaves (PREOG) for 4 days and then 50 mg/kg b.w. of CP was co-administered from day 5 to day 7 along with PREOG. Markers of renal function and oxidative stress, food and water intake, electrolytes, aldosterone, leukocytes infiltration, inflammation and histopathological alteration were evaluated.Results Obvious renal inflammation and kidney injuries were observed in CP treated groups. PREOG administration prevented oxidative stress, kidney injuries, attenuated inflammation, increased aldosterone production and reduced sodium and water loss in rats. PREOG also decreased the plasma concentrations of Interleukin-(IL)-6, C-reactive protein and activity of myeloperoxidase and malondialdehyde in CP treated rats.Conclusion OG prevented kidney injury and enhanced renal function via inhibiting inflammation and oxidant-induced CP toxicity. The efficacy of OG is related to the presence of various phytochemicals in the plant.
Development of a Method to Evaluate Influenza Virus RNA-dependent RNA Polymerase Activity Through Real-time Reverse Transcription Polymerase Chain Reaction
Authors: Horio Yuka , Shichiri Mototada , Isegawa Yuji 
Published: May 2021

Abstract
Background: The development of an influenza RNA-dependent RNA polymerase (RdRp) inhibitor is required; therefore, a method for evaluating the activity of influenza RdRp needs to be developed. The current method uses an ultracentrifuge to separate viral particles and quantifies RdRp activity with radioisotope-labeled nucleosides, such as 32P-GTP. This method requires special equipment and radioisotope management, so it cannot be implemented in all institutions. We have developed a method to evaluate the activity of RdRp without using ultracentrifugation and radioisotopes. Results: RdRp was extracted from viral particles that were purified from the culture supernatant using magnetic beads. A strand-specific real-time reverse transcription polymerase chain reaction (RT-PCR) method was developed based on the reverse transcription, using tagged primers. Real-time PCR using the tag sequence as the forward primer and a segment-specific reverse primer ensured the specificity for quantifying the mRNA of segments 1, 4, and 5. The temperature, reaction time, and Mg2+ concentration were determined to select the optimum conditions for in vitro RNA synthesis by RdRp, and the amount of synthesized mRNAs of segments 1, 4, and 5 was determined with a detection sensitivity of 10 copies/reaction. In addition, mRNA synthesis was inhibited by ribavirin triphosphate, an RdRp inhibitor, thus indicating the usefulness of this evaluation method for screening RdRp inhibitors.Conclusion: This method makes it possible to analyze the RdRp activity even in a laboratory where ultracentrifugation and radioisotopes cannot be used. This novel method for measuring influenza virus polymerase activity will further promote research to identify compounds that inhibit viral RNA synthesis.
Subjective Well-Being in Non-Obese Individuals in View of Abdominal Fat Distribution and Muscle Mass Index: A General Population Study.
Authors: Chlabicz Małgorzata , Paniczko Marlena , Jamiołkowski Jacek , Sowa Paweł , Szpakowicz Małgorzata , Łapińska Magda , Raczkowski Andrzej , Łaguna Wojciech , Moniuszko-Malinowska Anna M. , Waszkiewicz Napoleon , Kowalska Irina , Kamiński Karol A 
Published: May 2021

Abstract
Background

While obesity has been correlated with welfare in the general population, there is not much data on the influence of body composition on welfare among the non-obese adult individuals.
Methods

A longitudinal, population-based study was conducted in 2017–2020. Body composition was assessed using Dual Energy X-ray Absorptiometry. Welfare was rated with Satisfaction with Life Scale (SWLS), Euro Quality of Life Visual Analogue Scale (EQ-VAS) and Beck Depression Inventory (BDI).
Results

A total of 726 non-obese individuals from general population were analyzed. The mean age was 46.8 ± 15.4 years and 42.1% of participants were male. The mean value for SWLS was 23.09 ± 5.43, for EQ-VAS was 78.0 ± 14.5, and for BDI was 6.7 ± 6.6. On SWLS the waist-hip ratio had a negative impact even after adjustment for age, gender and concomitant diseases. EQ-VAS was inversely associated with android fat distribution and directly associated with muscle mass. BDI value was inversely associated with lower muscle mass, especially of the lower limbs. The well-being of women was mainly associated with the distribution of adipose tissue and less with the distribution of muscle tissue - abdominal fat distribution has a particularly negative impact on well-being among women. In contrast, men's well-being depends more on muscle mass and to a lesser extent on the distribution of fat tissue - a positive significant effect has lean mass and a circumference of thigh below gluteal fold.
Conclusions

Body composition is significantly associated with welfare in non-obese population. Android type fat distribution may have a negative impact while muscle mass positive.
Long Noncoding RNA LINC00460 Facilitates the Proliferation and Metastasis of Renal Cell Carcinoma via PI3K/AKT Signaling Pathway
Authors: Zhou Feng-Juan , Meng Sen , Yong Hongmei , Hou Ping-Fu , Li Min-Le , Chu Su-Fang , Bai Jin , Zheng Jun-Nian 
Published: May 2021

Abstract
Renal cell carcinoma (RCC) is one of the most prevalent cancers. Long noncoding RNAs (LncRNAs) have been indicated as a mediator acted in tumorigenesis of RCC. However, the mechanism of LINC00460 on RCC is yet to be investigated. This study aimed to investigate the potential function of LINC00460 and underlying mechanism of RCC. We detected LINC00460 expression in RCC tissues and the prognosis in RCC patients using Gene Expression Profiling Interactive Analysis (GEPIA) website and The Cancer Genome Atlas (TCGA) database. LINC00460 level in normal renal cell line and RCC cell lines were detected by quantitative real-time polymerase chain reaction (qRT-PCR). We study the effects of LINC00460 on proliferation, migration, invasion, apoptosis in RCC cells lines using a series of in vivo and in vitro experiments. RNA sequencing (RNA-seq) analysis for the whole transcriptome was applied to searching potential LINC00460 related signal pathway in RCC. We identified the significant up-regulated expression level of LINC00460 in RCC tissues and cell lines. Elevated LINC00460 was correlated with shorter survival of RCC patients. Overexpression of LINC00460 promoted cell viability, proliferation, invasion and migration, while down-regulation of LINC00460 exerted inhibitory effect on these activities. We crucially identified that LNC00460 promotes development of RCC by influencing the PI3K/AKT pathway. Knockdown of LNC00460 decreased the phosphorylation of AKT and mTOR. The key finding of our study provided a new evidence suggesting that LINC00460 functions as an oncogene in RCC pathogenesis by mediating the PI3K/AKT pathway, which may provide a new target for the treatment of RCC.