bluuebunny/crossref_metadata_2025_split huggingface dataset:
Salvia Chinensia Benth Inhibits Triple Negative Breast Cancer Progression Through Inducing DNA Damage Pathway
Authors: Wang Kainan , Hu Ye , Xu Lingzhi , Zhao Shanshan , Song Chen , Sun Siwen , Zhao Zuowei , Li Man 
Published: July 2021

Abstract
Background: Salvia chinensia Benth (SCH) is an herb that has extensively demonstrated antitumor attributes in multiple tumors. However, there are only marginal reports about the impacts of SCH on TNBC-triple negative breast cancer tumorigenesis. Herein, our current research reported the in vitro effects as well as the in vivo antitumor effect, and the fundamental mechanisms of SCH on TNBC. Methods: In vitro, we measured the antitumor effect of SCH on TNBC through clonogenic assay, cell viability, flow cytometry, apoptosis assay, migration and invasion assay. Meanwhile, breast cancer xenografts with the aid of MDA-MB-231 cells were established to evaluate the therapeutic effect of SCH on TNBC in vivo. To investigate the molecular mechanisms of SCH, bioinformatic analysis, specifically network pharmacology-based analysis, was performed. Then, the comet assay and western blot analyses were applied to confirm the influence of SCH on the DNA damage and repair pathways.Results: Our results demonstrated that the application of SCH could lead to the reticence of cell proliferation, migration, invasion and the induction of cellular apoptosis caused in TNBC cells. Based on the bioinformatic analysis, we discovered two key compounds of SCH, quercetin and beta-sitosterol, and detected significant synergistic effects of the combined treatment of them on TNBC, especially on the DNA damage of TNBC cells as judged by comet assays. Moreover, substantial inductions of DNA damage were examined after SCH treatment in TNBC cells, as well as quercetin, and beta-sitosterol treatment. Apart from inducing DNA damage, SCH specifically decreased inter-strand crosslink repair pathway and the double-strand breach repair perforated by the homologous recombination pathway. Similar results were also obtained with quercetin and beta-sitosterol treatment. Conclusion: Taken together, the current investigation elucidates the novel discernment into the therapeutic function of SCH as a DNA damage agent for treatment of TNBC.
Conventional Multiplex PCR For Identify Soil Transmitted Helminthes (Ascais Lumbricoides, Trichuris Trichura And Ancylostoma Duodenale) In Fecal Specimens
Authors: Alomashi Ghada Basi Ali , Khudhur Hasan Raheem , Jebur Layla Safar 
Published: July 2021

Abstract
Ascaris lumbricoides, Trichuris trichiura, and Ancylostoma duodenale are soil-transmitted helminths (STHs) and medically neglected in Iraq country in spite of their effect on the public health. A cross-sectional study was performed in the Maternity and Childhood Teaching Hospital and General Education Hospital in Al-Dewanyia province, included 850 tool samples collected from patients who attended to the O&P lab. General stool examination (GSE), Direct wet mount method DWMM and Kato-Katz were using for diagnosis of STH infections through detected the adult and the ovum of the helminthes. A conventional multiplex PCR assay was used for detection of STHs in fecal samples. Base on microscopic examination. The results showed that 275/ 850 range among triple, double and single infection on other hand was 365/ 850 range among triple, double and single infection. In conclusions the investigative sensitivity of DWMM is notable for STH, in exception, it is capable to identify patients with the intention of highest required of management, and therefore contributes to the universal target to reduce STH as a community healthiness trouble.
ANXA2 is Correlated With the Molecular Features and Clinical Prognosis of Glioma, and Acts as a Potential Marker of Immunosuppression
Authors: Ma Kaiming , Chen Xin , Liu Weihai , Yang Yang , Chen Suhua , Sun Jianjun , Ma Changcheng , Wang Tao , Yang Jun 
Published: July 2021

Abstract
Background: Recent studies have shown that ANXA2 plays a crucial role in the development of many cancers, while its role in glioma remains unclear. Thus, our study aimed to investigate the molecular biological characteristics and clinical value of ANXA2 in glioma. Methods: We analyzed the RNA sequencing data of 325 glioma samples from the Chinese Glioma Genome Atlas (CGGA) as the training cohort and the RNA expression data of 699 samples from The Cancer Genome Atlas (TCGA) database as the validation cohort, totaling approximately 1024 samples. Results: We found that ANXA2 was overexpressed significantly in high-grade glioma (HGG), isocitrate dehydrogenase (IDH) wild-type glioma and mesenchymal-subtype glioma. Receiver operating characteristic (ROC) analysis revealed that ANXA2 was a potential indicator of the mesenchymal subtype. Kaplan–Meier curve analysis revealed that high expression of ANXA2 indicated significantly poorer survival in glioma and HGG patients. Multivariate Cox analysis also demonstrated that ANXA2 acted as an independent indicator of poor prognosis in glioma patients. Further gene ontology (GO) analysis showed that ANXA2-related genes were mainly involved in the innate immune response and inflammatory response of glioma, and these genes were positively correlated with the expression of ANXA2. Then, we selected seven immune-related meta-genes and found that ANXA2 was positively correlated with HCK, LCK, MHC II, STAT1 and interferon but negatively correlated with IgG. Subsequent correlation analysis of infiltrating immune cells showed that ANXA2 was positively correlated with the infiltration of tumor-related macrophages (TAMs), regulatory T cells (Tregs) and myeloid-derived suppressor cells (MDSCs), suggesting that ANXA2 may be involved in the immunosuppression of glioma.Conclusion: Our study revealed that ANXA2 is closely related to the malignant glioma phenotype and poor patient prognosis. ANXA2 also plays an important role in the immune response and inflammatory activity and can be considered a biomarker of immunosuppression in glioma. These findings suggest that ANXA2 may be a promising target for the further development of immunotherapeutic for glioma.
The rich get richer and the poor get poorer: commonness and rarity of arctic tundra plants diverge under warming and herbivore exclusion
Authors: Post Eric , Pedersen Christian , Watts David A. 
Published: July 2021

Abstract
While most species are rare, our understanding of how rare species persist remains limited. Consequently, little is also known about how the commonness and rarity of co-occurring species might be differentially impacted by direct and indirect effects of climate change. We report results of a 15-year field experiment investigating effects on commonness and rarity of 14 arctic tundra plant taxa to warming and exclusion of large herbivores, factors demonstrated to have important effects on plant community composition in many biomes. Across all taxa, pooled commonness was reduced by experimental warming, and more strongly under herbivore exclusion than under herbivory. However, taxon-specific analyses revealed that although warming elicited variable effects on commonness, herbivore exclusion disproportionately reduced the commonness of rare taxa. Over the course of the experiment, we also observed trends in commonness and rarity under all treatments through time. Sitewide commonness increased for two common taxa, the deciduous shrubs Betula nana and Salix glauca, and declined in six other taxa, all of which were rare. Across experimental treatments, rates of increase and decline in commonness (i.e., temporal trends over the duration of the experiment) were strongly related to baseline commonness of taxa early in the experiment. Hence, commonness itself may be a strong predictor of plant species responses to climate change in the arctic tundra biome, but large herbivores may mediate such responses in rare taxa, perhaps facilitating their persistence.
Imaging and reconstruction of the cytoarchitecture of axonal fibres: enabling biomedical engineering studies involving brain microstructure
Authors: Bernardini Andrea , Trovatelli Marco , Klosowski Michal , Pederzani Matteo , Zani Davide , Brizzola Stefano , Porter Alexandra , Baena Ferdinando Rodriguez y , Dini Daniele 
Published: July 2021

Abstract
There is an increased need and focus to understand how local brain microstructure affects the transport of drug molecules directly administered to the brain tissue, for example in convection-enhanced delivery procedures. This study reports the first systematic attempt to characterize the cytoarchitecture of commissural, long association and projection fibers, namely: the corpus callosum, the fornix and the corona radiata. Ovine samples from three different subjects were stained with osmium tetroxide (to enhance contrast from cell organelles and the fibers), embedded in resin and then imaged using scanning electron microscope combined with focused ion beam milling to generate 3D volume reconstructions of the tissue at subcellular spatial resolution. Particular focus has been given to the characteristic cytological feature of the white matter: the axons and their alignment in the tissue. Via 2D images a homogeneous myelination has been estimated via detection of ~40% content of lipids in all the different fiber tracts. Additionally, for each tract, a 3D reconstruction of relatively large volumes (15μm x 15μm x 15μm – including a significant number of axons) has been performed. Namely, outer axonal ellipticity, outer axonal cross-sectional area and their relative perimeter have been measured. The study of well-resolved microstructural features provides useful insight into the fibrous organization of the tissue, whose micromechanical behaviour is that of a composite material presenting elliptical tortuous tubular fibers embedded in the extra-cellular matrix. Drug flow can be captured through microstructurally-based models, leading to a workflow to enable physically-accurate simulations of drug delivery to the targeted tissue.
A IMPORTÂNCIA DA INSERÇÃO DA CULTURA DA PAZ NO CURRÍCULO ESCOLAR
Authors: Monteiro Michelle Popenga Geraim , Lima-Berton Tatiane Delurdes de , Asinelli-Luz Araci 
Published: July 2021

O objetivo do presente estudo é salientar a importância da inserção da Cultura da Paz no currículo escolar para a prevenção das violências na infância no ambiente escolar, visto que a escola tem sido considerada como espaço de expressão e manifestação da violência. É resultado de um estudo qualitativo e tem como metodologia o estudo bibliográfico, decorrentes de pesquisas de abordagem teórica, relacionadas ao Mestrado em Educação da Universidade Federal do Paraná. No contexto social e educacional, em defesa de uma escola com base humanizadora, amplia-se o olhar para o âmbito do desenvolvimento do currículo escolar, aliado à Cultura da Paz como forma de compreensão e prevenção do fenômeno das violências. Acredita-se que ao compreender a escola como espaço de interações, cujos valores são pautados no diálogo e no respeito, aumenta-se a chance concreta de uma realidade educativa melhor e de prevenção das violências.
CURSO NORMAL RURAL DO COLÉGIO NOSSA SENHORA AUXILIADORA DE PETROLINA (1929-1989): UM MARCO NA HISTÓRIA DA FORMAÇÃO DOCENTE
Authors: Mendes da Silva Rosa Santos , Reis Edmerson dos Santos 
Published: July 2021

RESUMO
Neste trabalho apresentamos uma trajetória histórica do Curso Normal Rural do Colégio Nossa Senhora Auxiliadora. Essa Instituiçãose enquadra dentro de uma perspectiva de ensino confessional, mantida pela congregação das filhas de Maria Auxiliadora, constituída nos conceitos cristãos fundada na primeira metade do século XX.Com relação aos aspectos teórico-metodológicos, a pesquisa baseou-se na análise histórico documental e pesquisa de campo, tendo como base o recorte temporal as décadas de 1929 a 1989, período que abrange desde a criação do curso normal rural até o ano em que houve oficialmente a extinção do curso. Foi possível apreender que no estado de Pernambuco a primeira “Escola Normal Rural” foi a do Colégio Nossa Senhora Auxiliadora no ano de 1936. Assim, a educação do Curso Normal esteve associada às idéias de cristianização da Igreja Católica e atendeu os anseios da sociedade da época.
PALAVRAS-CHAVE: Curso Normal Rural.Educação Confessional. Formação docente.
Influence of Metabolic Syndrome on Factors Associated with Chronic Kidney Disease: A Cross-sectional Study
Authors: Marreiros Camila Santos , Nogueira Thaís Rodrigues , do Nascimento Paulo Pedro , de Araújo Diana Stefany Cardoso , do Nascimento Monteiro Nayara Vieira , da Paz Suzana Maria Rebelo Sampaio , de Jesus e Silva de Almendra Freitas Betânia 
Published: July 2021

Background:
Metabolic Syndrome (MetS) is defined by the presence of three or more
of the following components: inadequate fasting serum glucose levels and elevated waist circumference,
hypertension and dyslipidemia, which represent a potential risk for the development
and/or worsening of Chronic Kidney Disease.

Objective:
Based on this, this research aimed to investigate the presence of MetS and its influence
on associated factors in patients with Chronic Kidney Disease undergoing hemodialysis.

Methods:
This is an evaluation of a cross-sectional multicenter research project, carried out with
95 patients with Chronic Kidney Disease, seen at outpatient clinics in the state capital Piaui. Anthropometric,
biochemical and hemodynamic parameters were determined. The data were analyzed
using the Stata® v.12 software (Statacorp, College Station, Texas, USA), adopting a significance
level of p < 0,05. The survey received ethical approval (no. 2.527.329).

Results:
It was observed that individuals with elevated BMI, WC, NC, SBP, DBP were more
likely to develop MetS, with significant differences (p <0.001). In addition, it was found that
serum levels of glucose, insulin, HOMA-IR, TC, LDL, TG and blood pressure were higher in the
group with MetS.

Conclusion:
It was concluded that changes in the parameters analyzed in patients with CKD reinforce
MetS as a predictive condition for worsening nutritional status and a factor for the progression
of kidney disease.
The Use of Mobile Phones by Women Accessing Health Care Services in
Bangladesh: A Cross-Sectional Study
Authors: Hossain Md. Mosharaf , Min Ruhani Mat , Shukri Madihah Mohd 
Published: July 2021

Background:
Maternal and neonatal mortality rates still pose a global challenge.

Objectives:
The present study aimed to identify the socio-demographic characteristics of mobile users trying to access antenatal care services, transportation to facilities, and postnatal care for mothers and newborns.

Methods:
This was a cross-sectional study that used data collected from the Bangladesh Demographic and Health Survey (BDHS) 2014. Data were gathered using a questionnaire. Participants comprised 4,494 mothers aged between 14 and 49. Models of logistic regression were used to determine associations.

Results:
The overall sample size was 4494, with only 22.23% of women using mobile phones. Among these, 29.9% used their mobile phones to obtain health services or advice and 70.1% did not. Women with mobile phones who had a higher level of education used them to receive more health care (secondary and higher: OR = 1.922, 95%CI = 1.225−3.014; primary: OR = 1.982, 95% CI = 1.738−2.654); they were of higher socio-economic status (rich: OR = 1.228, 95% CI = 1.009&−1.494; middle income: OR = 1.691, 95% CI = 1.256−2.333); they accessed more prenatal care (yes: OR = 1.993, 95% CI = 1.425−1.987) and antenatal care (yes: OR = 1.951, 95% CI = 1.765−1.998); they checked their health status after delivery (yes: OR = 1.966, 95% CI = 1.639−2.357).

Conclusion:
The results of the present study showed that young women who had a higher level of education and income and resided in urban areas were more likely to use a mobile phone to receive health care facilities in Bangladesh. Intervention studies are essential in recognising that mobile phone-based facilities improve maternal health care.
Prediction of Asthma Hospitalizations for the Common Cold Using Google Trends: Infodemiology Study
Authors: Sousa-Pinto Bernardo , Halonen Jaana I , Antó Aram , Jormanainen Vesa , Czarlewski Wienczyslawa , Bedbrook Anna , Papadopoulos Nikolaos G , Freitas Alberto , Haahtela Tari , Antó Josep M , Fonseca João Almeida , Bousquet Jean 
Published: July 2021

Background
In contrast to air pollution and pollen exposure, data on the occurrence of the common cold are difficult to incorporate in models predicting asthma hospitalizations.

Objective
This study aims to assess whether web-based searches on common cold would correlate with and help to predict asthma hospitalizations.

Methods
We analyzed all hospitalizations with a main diagnosis of asthma occurring in 5 different countries (Portugal, Spain, Finland, Norway, and Brazil) for a period of approximately 5 years (January 1, 2012-December 17, 2016). Data on web-based searches on common cold were retrieved from Google Trends (GT) using the pseudo-influenza syndrome topic and local language search terms for common cold for the same countries and periods. We applied time series analysis methods to estimate the correlation between GT and hospitalization data. In addition, we built autoregressive models to forecast the weekly number of asthma hospitalizations for a period of 1 year (June 2015-June 2016) based on admissions and GT data from the 3 previous years.

Results
In time series analyses, GT data on common cold displayed strong correlations with asthma hospitalizations occurring in Portugal (correlation coefficients ranging from 0.63 to 0.73), Spain (ρ=0.82-0.84), and Brazil (ρ=0.77-0.83) and moderate correlations with those occurring in Norway (ρ=0.32-0.35) and Finland (ρ=0.44-0.47). Similar patterns were observed in the correlation between forecasted and observed asthma hospitalizations from June 2015 to June 2016, with the number of forecasted hospitalizations differing on average between 12% (Spain) and 33% (Norway) from observed hospitalizations.

Conclusions
Common cold–related web-based searches display moderate-to-strong correlations with asthma hospitalizations and may be useful in forecasting them.
Feasibility of Indirect Secondary Distribution of HIV Self-test Kits via WeChat Among Men Who Have Sex With Men: National Cross-sectional Study in China
Authors: Li Shangcao , Zhang Jing , Mao Xiang , Lu Tianyi , Gao Yangyang , Zhang Wenran , Wang Hongyi , Chu Zhenxing , Hu Qinghai , Jiang Yongjun , Geng Wenqing , Shang Hong , Xu Junjie 
Published: July 2021

Background
HIV self-testing (HIVST) kits are common in key sexually active populations. Direct secondary distribution of HIVST kits (DSDHK) is effective in improving the uptake of HIVST. However, there are concerns about the various limitations of DSDHK, including limited geographic reach, payment problems, and need for face-to-face interactions.

Objective
In this study, we aim to evaluate the feasibility and characteristics of indirect secondary distribution of HIVST kits (ISDHK) via WeChat (distributing HIVST application links and follow-up HIVST kits to partners) among men who have sex with men (MSM).

Methods
From October 2017 to September 2019, an HIVST recruitment advertisement was disseminated on the WeChat social media platform to invite MSM to apply for the HIVST kits (referred to as index participants [IPs]). All MSM participants were encouraged to distribute the HIVST application link to their friends and sexual partners (referred to as alters) through their social networks. All the alters were further encouraged to continue distributing the HIVST application link. All participants paid a deposit (US $7), which was refundable upon completion of the questionnaire, and uploaded the test results via a web-based survey system.

Results
A total of 2263 MSM met the criteria and successfully applied for HIVST. Of these, 1816 participants returned their HIVST results, including 1422 (88.3%) IPs and 394 (21.7%) alters. More alters had condomless anal intercourse, a higher proportion of them had never previously tested for HIV, and they showed a greater willingness to distribute HIVST kits to their sexual partners (P=.002) than the IPs. After controlling for age, education, and income, the alters had a greater proportion of MSM who had never tested for HIV before (adjusted odds ratio [aOR] 1.29, 95% CI 1.00-1.68), were more willing to distribute the HIVST application link (aOR 1.71, 95% CI 1.21-2.40), had a lower number of sexual partners (aOR 0.71, 95% CI 0.57-0.90), and were less likely to search for sexual partners on the web (aOR 0.78, 95% CI 0.60-1.02) than IPs. In comparison, the rates of reactive HIVST results, conducting HIV confirmatory tests, HIV seropositivity, and initiation of HIV antiretroviral therapy were similar for IPs and alters.

Conclusions
The ISDHK model of distributing HIVST application links among the MSM population via social media is feasible. The ISDHK model should be used to supplement the DSDHK model to enable a greater proportion of the MSM population to know their HIV infection status.
Online Public Attention Toward Premature Ejaculation in Mainland China: Infodemiology Study Using the Baidu Index
Authors: Wei Shanzun , Ma Ming , Wen Xi , Wu Changjing , Zhu Guonian , Zhou Xiangfu 
Published: July 2021

Background
Premature ejaculation (PE) is one of the most described psychosocial stress and sexual complaints worldwide. Previous investigations have focused predominantly on the prospective identification of cases that meet researchers’ specific criteria. The genuine demand from patients with regard to information on PE and related issues may thus be neglected.

Objective
This study aims to examine the online search trend and user demand related to PE on a national and regional scale using the dominant major search engine in mainland China.

Methods
The Baidu Index was queried using the PE-related terms for the period of January 2011 to December 2020. The search volume for each term was recorded to analyze the search trend and demographic distributions. For user interest, the demand and trend data were collected and analyzed.

Results
Of the 36 available PE search keywords, 4 PE searching topics were identified. The Baidu Search Index for each PE topic varied from 46.30% (86,840,487/187,558,154) to 6.40% (12,009,307/187,558,154). The annual percent change (APC) for the complaint topic was 48.80% (P<.001) for 2011 to 2014 and –16.82% (P<.001) for 2014 to 2020. The APC for the inquiry topic was 16.21% (P=.41) for 2011 to 2014 and –11.00% (P<.001) for 2014 to 2020. For the prognosis topic, the annual APC was 11.18% (P<.001) for 2011 to 2017 and –19.86% (P<.001) for 2017 to 2020. For the treatment topic, the annual APC was 14.04% (P<.001) for 2011 to 2016 and –38.83% (P<.001) for 2016 to 2020. The age distribution of those searching for topics related to PE showed that the population aged 20 to 40 years comprised nearly 70% of the total search inquiries (second was 17.95% in the age group younger than 19 years). People from East China made over 50% of the total search queries.

Conclusions
The fluctuating online popularity of PE searches reflects the real-time population demands. It may help medical professionals better understand population interest, population concerns, regional variations, and gender differences on a nationwide scale and make disease-specific health care policies. The internet search data could be more reliable when the insufficient and lagging registry data are completed.