bluuebunny/crossref_metadata_2025_split huggingface dataset:
Fatiga y Calidad de vida en niños, niñas y adolescentes con cáncer hematológico
Authors: Espín Jordán Javier Andrés , Camacho Rodriguez Erick Andrés , Jurado Auria Stalin Augusto 
Published: July 2021

Introducción: La fatiga es un problema relevante en pacientes con cáncer hematológico y puede ser un motivo de reducción importante de la calidad de vida. El objetivo del presente estudio fue realizar un estudio descriptivo de un grupo de niños y adolescentes portadores de neoplasias de origen hematológico en un centro único de referencia.
Metodología: El presente estudio observacional tiene un corte retrospectivo, en donde se incluyeron pacientes menores a 18 años con el diagnóstico de cáncer hematológico en quienes se revisó la historia clínica y se realizó un test de calidad de vida. Se realiza un análisis de correlación entre las variables usando estadística no paramétrica.
Resultados: ingresaron al estudio 64 pacientes. De sexo masculino (n=37) y femenino (n=27). El valor medio de fatiga fue (5.72 ± 0.53 y 5.85 ± 0.51), en actividad física y salud (1.49 ± 0.17 y 1.44 ± 0.13) en hombres y mujeres respectivamente. El valor medio de edad fue de 5.81 ± 2.46 en el rango de 2 a 10 años y 14.55 ±2.15 en el rango de 11 a 18 años. Según el rango etario existió más predominancia de fatiga en niños que en adolescentes y más en mujeres que en hombres.
Conclusión: No se encontró relación entre las variables de estudio. Sin embargo, los hallazgos expuestos indican una marcada sensación de fatiga siendo esta moderada pudiendo convertirse en seve-ra; de igual manera se obtuvo niveles bajos y medios de calidad de vida en las similares di-mensiones incluidas en las baterías de evaluación; sugiriendo futuras investigaciones inclu-yendo colaboración de abordaje multidisciplinaria para llegar a impartir las terapias adecuadas que beneficien a los pacientes con cáncer.
The Regulatory Network and Potential Role of LINC00973-miRNA-mRNA ceRNA in the Progression of Non-Small-Cell Lung Cancer
Authors: Guo Qiang , Li Dan , Luo Xiangyu , Yuan Ye , Li Tian , Liu Huasong , Wang Xinju 
Published: July 2021

BackgroundThe occurrence and development of cancer could be promoted by abnormally competing endogenous RNAs (ceRNA) network. This article aims to determine the prognostic biomarker of ceRNA for non-small-cell lung cancer (NSCLC) prognosis.MethodsThe expression and clinical significance of LINC00973 in NSCLC tissues were analyzed via the The Cancer Genome Atlas (TCGA), Gene Expression Profiling Interactive Analysis (GEPIA), lnCAR, and clinical samples in Taihe Hospital. The biological functions and signaling pathways involved in target genes of ceRNA network were analyzed via Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG). Survival analysis, univariate and multivariate Cox regression analysis were used for prognostic-related mRNA.ResultsExpression of LINC00973 was increased in NSCLC tissues. High expression of LINC00973 was associated with poor prognosis of NSCLC patients. There were 15 miRNA and 238 differential mRNA in the INC00973-miRNA-mRNA ceRNA network, involving cell migration, endothelial cell proliferation, tumor growth factor (TGF)-β, cellular senescence, phosphatidylinositol 3-hydroxy kinase (PI3K)-Akt, Hippo, Rap1, mitogen-activated protein kinase (MAPK), cell cycle signaling pathway, etc. The expression levels of RTKN2, NFIX, PTX3, BMP2 and LOXL2 were independent risk factors for the poor prognosis of NSCLC patients.ConclusionsLINC00973-miRNA-mRNA ceRNA network might be the basis for determining pivotal post-translational regulatory mechanisms in the progression of NSCLC. BMP2, LOXL2, NFIX, PTX3 and RTKN2 might be valuable prognostic markers and potential therapeutic targets.
Clinical Significance and Inflammatory Landscape of aNovel Recurrence-Associated Immune Signature in Stage II/III Colorectal Cancer
Authors: Liu Zaoqu , Lu Taoyuan , Li Jing , Wang Libo , Xu Kaihao , Dang Qin , Liu Long , Guo Chunguang , Jiao Dechao , Sun Zhenqiang , Han Xinwei 
Published: July 2021

BackgroundA considerable number of patients with stage II/III colorectal cancer (CRC) will relapse within 5 years after surgery, which is a leading cause of death in early-stage CRC. The current TNM stage system is limited due to the heterogeneous clinical outcomes displayed in patients of same stage. Therefore, searching for a novel tool to identify patients at high recurrence-risk for improving post-operative individual management is an urgent need.MethodsUsing four independent public cohorts and qRT-PCR data from 66 tissues, we developed and validated a recurrence-associated immune signature (RAIS) based on global immune genes. The clinical and molecular features, tumor immune microenvironment landscape, and immune checkpoints profiles of RAIS were also investigated.ResultsIn five independent cohorts, this novel scoring system was proven to be an independent recurrent factor and displayed excellent discrimination and calibration in predicting the recurrence-risk at 1~5 years. Further analysis revealed that the high-risk group displayed high mutation rate of TP53, while the low-risk group had more abundance of activated CD4+/CD8+ T cells and high expression of PD-1/PD-L1.ConclusionsThe RAIS model is highly predictive of recurrence in patients with stage II/III CRC, which might serve as a powerful tool to further optimize decision-making in adjuvant chemotherapy and immunotherapy, as well as tailor surveillance protocol for individual patients.
Desaturation-Distance Ratio During Submaximal and Maximal Exercise Tests and Its Association With Lung Function Parameters in Patients With Lymphangioleiomyomatosis
Authors: Queiroz Douglas Silva , da Silva Cibele Cristine Berto Marques , Amaral Alexandre Franco , Oliveira Martina Rodrigues , Moriya Henrique Takachi , Carvalho Carlos Roberto Ribeiro , Baldi Bruno Guedes , Carvalho Celso R. F. 
Published: July 2021

Background: The desaturation–distance ratio (DDR), the ratio of the desaturation area to the distance walked, is a promising, reliable, and simple physiologic tool for functional evaluation in subjects with interstitial lung diseases. Lymphangioleiomyomatosis (LAM) is a rare neoplastic condition frequently associated with exercise impairment. However, DDR has rarely been evaluated in patients with LAM.Objectives: To assess DDR during maximal and submaximal exercises and evaluate whether DDR can be predicted using lung function parameters.Methods: A cross-sectional study was conducted in a cohort of women with LAM. The 6-min walking test (6MWT) and the incremental shuttle walking test (ISWT) were performed, and DDR was obtained from both tests. The functional parameters were assessed at rest using spirometry and body plethysmography. The pulmonary function variables predictive of DDR were also assessed.Results: Forty patients were included in this study. The mean age was 46 ± 10 years. Airway obstruction, reduced DLCO, and air trapping were found in 60, 57, and 15% of patients, respectively. The distance walked and the DDR for the 6MWT and ISWT were, respectively, 517 ± 65 and 443 ± 127 m; and 6.6 (3.8–10.9) and 8.3 (6.2–12.7). FEV1 (airway obstruction) and reduced DLCO and RV/TLC (air trapping) were independent variables predictive of DDR during exercises field tests [DDR6MWT = 18.66–(0.06 × FEV1%pred)–(0.10 × DLCO%pred) + (1.54 × air trapping), Radjust2 = 0.43] and maximal [DDRISWT = 18.84–(0.09 × FEV1%pred)–(0.05 × DLCO%pred) + (3.10 × air trapping), Radjust2 = 0.33].Conclusion: Our results demonstrated that DDR is a useful tool for functional evaluation during maximal and submaximal exercises in patients with LAM, and it can be predicted using airway obstruction, reduced DLCO, and air trapping.
Cancer Cells Can Exhibit a Sparing FLASH Effect at Low Doses Under Normoxic In Vitro-Conditions
Authors: Adrian Gabriel , Konradsson Elise , Beyer Sarah , Wittrup Anders , Butterworth Karl T. , McMahon Stephen J. , Ghita Mihaela , Petersson Kristoffer , Ceberg Crister 
Published: July 2021

BackgroundIrradiation with ultra-high dose rate (FLASH) has been shown to spare normal tissue without hampering tumor control in several in vivo studies. Few cell lines have been investigated in vitro, and previous results are inconsistent. Assuming that oxygen depletion accounts for the FLASH sparing effect, no sparing should appear for cells irradiated with low doses in normoxia.MethodsSeven cancer cell lines (MDA-MB-231, MCF7, WiDr, LU-HNSCC4, HeLa [early passage and subclone]) and normal lung fibroblasts (MRC-5) were irradiated with doses ranging from 0 to 12 Gy using FLASH (≥800 Gy/s) or conventional dose rates (CONV, 14 Gy/min), with a 10 MeV electron beam from a clinical linear accelerator. Surviving fraction (SF) was determined with clonogenic assays. Three cell lines were further studied for radiation-induced DNA-damage foci using a 53BP1-marker and for cell cycle synchronization after irradiation.ResultsA tendency of increased survival following FLASH compared with CONV was suggested for all cell lines, with significant differences for 4/7 cell lines. The magnitude of the FLASH-sparing expressed as a dose-modifying factor at SF=0.1 was around 1.1 for 6/7 cell lines and around 1.3 for the HeLasubclone. Similar cell cycle distributions and 53BP1-foci numbers were found comparing FLASH to CONV.ConclusionWe have found a FLASH effect appearing at low doses under normoxic conditions for several cell lines in vitro. The magnitude of the FLASH effect differed between the cell lines, suggesting inherited biological susceptibilities for FLASH irradiation.
CDK4: A Novel Therapeutic Target for Extramammary Paget’s Disease
Authors: Hashimoto Hiroki , Kaku-Ito Yumiko , Oda Yoshinao , Ito Takamichi 
Published: July 2021

BackgroundThe outcome of extramammary Paget’s disease (EMPD) is poor when it progresses to metastasis because of the lack of effective systemic therapies. Recently, CDK4-targeted therapy has attracted attention as a potential therapeutic target for some cancers. The aim of this study was to analyze the impact of CDK4 expression on the survival of patients with EMPD.MethodsWe retrospectively reviewed 110 patients with EMPD. We conducted immunohistochemical analysis of CDK4 and cyclin D1 expression, and assessed the association between their expression and survival.ResultsMost EMPD lesions (108/110, 98.2%) were positive for CDK4 staining and there was a positive correlation between CDK4 expression and cyclin D1 expression (r = 0.54,p&lt; 0.001). Tumor thickness (p= 0.0003) and the presence of regional lymph node metastasis (p= 0.015) were significantly associated with high CDK4 expression. Regarding invasive EMPD, the multivariate analysis did not show the correlation between the expression of CDK4/cyclin D1 and survival outcomes (HR: 3.14,p= 0.14).ConclusionThe overexpression of CDK4 was identified as a major risk factor for disease progression. CDK4-targeted therapy could thus be a novel treatment option for unresectable EMPD.
Comprehensive Analysis of the Expression Profiles of Hepatic lncRNAs in the Mouse Model of Alcoholic Liver Disease
Authors: Dou Xiaobing , Yang Wenwen , Ding Qinchao , Han Qiang , Qian Qianyu , Du Zhongyan , Fan Yibin , Wang Cui , Li Songtao 
Published: July 2021

Background and Aim: The worldwide prevalence of alcoholic liver disease (ALD) due to escalating alcohol consumption has presented an unprecedented pressure on human health. A few studies have determined long non-coding RNAs (lncRNAs) involved in the pathogenesis of liver diseases. However, the roles of lncRNAs in ALD development is still poorly understood.Methods: An ALD mouse model was established and confirmed. Expression profiles of lncRNAs were obtained by whole transcriptome sequencing. The altered lncRNAs in ALD mice were further verified by qRT-PCR. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses were used to enrich the functions of these lncRNAs. In combination with miRNA and mRNA profiles, we constructed concise endogenous RNA (ceRNA) networks. The function of the most up/downregulated lnRNA was further verified and investigated in both ALD model and AML-12 cells.Results: Totally, five downregulated lncRNAs were obtained and verified in ALD mice. The GO term and KEGG pathway analyses revealed that the identified lncRNAs were associated with alcohol-induced hepatic oxidative damage, cellular inflammation, and lipid metabolism. Combination the differentially modulated miRNAs and mRNAs with ceRNA network analysis, we constructed five ceRNA networks and obtained 30 miRNAs and 25 mRNAs that may participate in ALD. Further, we verified and investigate the function of the most downregulated lnc_1700023H06Rik. Depletion lnc_1700023H06Rik reduced genes encoding for lipid metabolism, especially mRNA Acat2 (ENSMUST00000159697) and Pgrmc2 (ENSMUST00000058578) both in vivo and in vitro. Knocking down lnc_1700023H06Rik induced triglyceride accumulation and lactate dehydrogenase leakage in AML12 cells, consisting with that in alcohol-treated cells.Conclusion: The five remarkably downregulated lncRNAs in ALD mouse model were identified as novel biomarkers, highlighting the key role of lncRNAs in the development of ALD. The effect of lnc_1700023H06Rik plays a pivotal role in lipid deposition and its pathological pathway in ALD needs further investigation.
Allogeneic Red Blood Cell Transfusion Rate and Risk Factors After Hemiarthroplasty in Elderly Patients With Femoral Neck Fracture
Authors: Yue Rui , Yang Minghui , Deng Xiaohui , Zhang Ping 
Published: July 2021

ObjectiveThis study aimed to determine the rate and risk factors of allogeneic red blood cell transfusions (ABT) after hemiarthroplasty (HA) in elderly patients with femoral neck fracture (FNF).MethodsThe subjects of the study were elderly patients (≥65 years old) who were admitted to the geriatric trauma orthopedics ward of Beijing Jishuitan Hospital between March 2018 and June 2019 for HA treatment due to an FNF. The perioperative data were collected retrospectively, and univariate and multivariate stepwise logistic regression analyses were performed to determine the post-operative ABT rate and its risk factors.ResultsThere were 445 patients in the study, of whom 177 (39.8%) received ABT after surgery. Multivariate stepwise logistic regression analysis showed that preoperative low hemoglobin (Hb), high intraoperative blood loss (IBL), advanced age, and a low body mass index (BMI) are independent risk factors of ABT after HA in elderly FNF patients.ConclusionABT after HA is a common phenomenon in elderly patients with FNF. Their post-operative ABT needs are related to preoperative low Hb, high IBL, advanced age, and low BMI. Therefore, ABT can be reduced by taking these factors into account. When the same patient had three risk factors (preoperative low hemoglobin, advanced age, and low BMI), the risk of ABT was very high (78.3%). Also, when patients have two risk factors of preoperative low hemoglobin and low BMI, the risk of ABT was also high (80.0%).
Management of the Cavity After Removal of Giant Cell Tumor of the Bone
Authors: Wang Yushan , Tian Qiaoqiao , Wu Chenyang , Li Haoze , Li Jian , Feng Yi 
Published: July 2021

Purpose: To find out the most appropriate management scheme through the analysis and comparison of different inactivation methods and filling materials.Method: A systematic literature search was performed using the terms, anhydrous ethanol, phenol, hypertonic saline, cryotherapy, thermal therapy, bone reconstruction, GCTB, and etc., Selected articles were studied and summarized. The mechanism, clinical effects, and influence on bone repair of various methods are presented. Recent developments and perspectives are also demonstrated.Recent Findings: Compared to curettage alone, management of the residual cavity can effectively reduce the recurrence of giant cell tumours of bone. It is a complex and multidisciplinary process that includes three steps: local control, cavity filling, and osteogenic induction. In terms of local control, High-speed burring can enlarge the area of curettage but may cause the spread and planting of tumour tissues. Among the inactivation methods, Anhydrous ethanol, and hyperthermia therapy are relatively safe and efficient. The combination of the two may achieve a better inactivation effect. When inactivating the cavity, we need to adjust the approach according to the invasion of the tumour. Filling materials and bone repair should also be considered in management.
Kepemimpinan Transformasional Dalam Pembentukan Provinsi Kepulauan Mentawai
Authors: Hutahaean Wendy Sepmady 
Published: July 2021

Abstract: As the times grew, leadership began to decline and crisis. This leadership crisis is also inseparable from the church environment. Speaking of leadership in the church is seen from the point of view of the Bible, inseparable from the Great person of Jesus Christ. This paper discusses Jesus' leadership model focusing on the Gospel of John. The author formulates the following problem: : What is the model of Jesus' leadership in the Gospel of John and its implementation in the local church? The purpose of writing this scientific paper is to know and explain the model of Jesus' leadership in the Gospel of John and its implementation in the local church. This study uses qualitative research method with descriptive approach which this method considers that the data collected to be the key to what is studied. The data the author observes is the Gospel of John, literature such as books and journal articles on leadership. The model of Jesus' leadership in the Gospel of John is Jesus as the Leader of the sheep, Jesus as the Ministering Leader, and Jesus discipleship and send. An example for the local church leadership model is to put self-integrity first, lead holistically, and implement a discipleship model. The local church leadership model reflecting Jesus' leadership model in the Gospel of John is expected to be a learning experience for leaders in the local church.
Keyword: model, the leadership of Jesus, the local church, the example.

Abstrak: Semakin berkembangnya zaman, kepemimpinan mulai merosot dan mengalami krisis. Krisis kepemimpinan ini pun juga tidak terlepas dari lingkungan gereja. Berbicara mengenai kepemimpinan dalam gereja dilihat dari sudut pandang Alkitab, tidak terlepas dari pribadi yang Agung yaitu Yesus Kristus. Penulisan ini membahas tentang model kepemimpinan Yesus yang berfokus pada Injil Yohanes. Penulis merumuskan masalah sebagai berikut: : Bagaimana model kepemimpinan Yesus dalam Injil Yohanes dan implementasinya dalam gereja lokal? Adapun tujuan penulisan dari karya ilmiah ini ialah untuk mengetahui dan memaparkan model kepemimpinan Yesus dalam Injil Yohanes dan implementasinya dalam gereja lokal. Penelitian ini menggunakan metode penelitian kualitatif dengan pendekatan deskriptif yang mana metode ini memandang bahwa data yang dikumpulkan menjadi kunci terhadap apa yang diteliti. Data yang penulis amati ialah kitab Injil Yohanes, literatur-literatur seperti buku dan artikel jurnal tentang kepemimpinan. Adapun model kepemimpinan Yesus dalam Injil Yohanes ialah Yesus sebagai Pemimpin domba-domba, Yesus sebagai Pemimpin yang melayani, dan Yesus memuridkan dan mengutus. Teladan bagi model kepemimpinan gereja lokal yaitu mengutamakan integritas diri, memimpin secara holistik, dan menerapkan model discipleship. Model kepemimpinan gereja lokal yang bercermin dari model kepemimpinan Yesus dalam Injil Yohanes diharapkan dapat menjadi pembelajaran bagi para pemimpin di gereja lokal.
Kata kunci: model, kepemimpinan Yesus, gereja lokal, teladan.
Tourist Perceptions About the Implementation of CHSE in Greater Bandung: A Preliminary Study
Authors: Ningrum Harini Fajar , Septyandi Chandra Budhi 
Published: July 2021

This research is based on the phenomenon of a shift in tourist behaviour during the Covid-19 pandemic. Cleanliness, Health, Safety, and Environment (CHSE) are the primary considerations for tourists when deciding to choose a tourist destination while on vacation, beating other aspects such as low prices. This study provides an initial picture of how tourists perceive the application of CHSE in a tourist destination. The method used in this research is descriptive quantitative through a survey using a questionnaire, then the results are tabulated into a frequency distribution table. The results of this study indicate that according to the perception of tourists who are dominated by the Millennials market segment, assessing that the application of CHSE in destinations around Bandung Raya is in the category of fair.
Keywords: tourist perception, CHSE, Cleanliness, Health, Safety
ENSINO DE GRAMÁTICA: FORMAS REMISSIVAS LEXICAIS E FORMAÇÃO DE SENTIDO
Authors: Pontes Leila Silvana , Silva Filho Marcelo Nicomedes dos Reis 
Published: July 2021

O presente estudo traz para discussão a renitente postura de ensino de gramática presa a regras e a normas que não contribuem para o crescimento linguístico do aluno. A fim de defender um ensino diferente, que privilegie o uso, os sujeitos e a reflexão sobre aspectos linguísticos, este trabalho propõe uma prática de ensino de gramática pautada na Linguística Textual. Para isso, trabalhou coesão textual, especificamente, formas remissivas lexicais. Procurou resposta ao seguinte questionamento: Como o trabalho com gramática, a partir da Linguística Textual, corrobora para o entendimento de que escolhas lexicais mostram que os sujeitos são atuantes, sócio e historicamente determinados? A análise, bibliográfica e qualitativa, buscou, dentre outros autores, fundamentação teórica, principalmente, em Ingedore Koch (2001; 2006; 2009; 2012; 2014). Após levantamento teórico, analisou-se um texto de autor indígena, considerando algumas subclassificações das formas remissivas lexicais:1) Expressões ou Grupos Nominais Definidos; 2) Nominalizações; 3) Expressões Sinônimas ou Quase Sinônimas”; 4) “Hiperônimos ou Indicadores de Classe”. Objetivou-se com isso analisar como o ensino de gramática contribui para o entendimento das escolhas lexicais enquanto ação, identificação e determinação do sujeito. As análises mostraram que o trabalho com a coesão não precisa ser mecânico e que as expressões, bem como o léxico não estão fadados a um sentido fechado, uma vez que são clivados pela história, contexto e intenções. Revelou que o sujeito, ao fazer uso das formas remissivas lexicais, usa o referente conforme enxerga o seu universo ao interagir sociocognitivamente com ele. As escolhas não são aleatórias, além de serem ações do sujeito revelam quem eles são, identificando-os e determinando-os.
REATOR UASB: UMA ALTERNATIVA SUSTENTÁVEL PARA O TRATAMENTO DE ESGOTO NO MUNICÍPIO DE CODÓ – MA
Authors: Da Silva Sidne Rodrigues , Torres Michele Sousa Travassos , Rocha Nayra Salazar 
Published: July 2021

Diversos são os fatores que constituem risco à saúde pública no Brasil. Dentre eles, destacam-se as condições precárias de saneamento básico. Este estudo teve como objetivo principal avaliar melhorias nas condições de saneamento do município de Codó, Maranhão, através da construção do protótipo de um reator UASB (Upflow Anaerobic Sludge Blanket) destinado à remoção de matéria orgânica do esgoto doméstico que é lançado diretamente no Rio Itapecuru, o qual corta a cidade de Codó - MA. A pesquisa de campo foi realizada através de questionários dirigidos a líderes comunitários (vinte sujeitos) e agentes de saúde (trinta sujeitos) de cada bairro. O protótipo de reator UASB construído em cano plástico PVC teve a avaliação da sua eficiência indicada a partir dos parâmetros: Demanda Química de Oxigênio (DQO) e Demanda Bioquímica de Oxigênio (DBO) de amostras do efluente doméstico bruto e filtrado. Os dados da pesquisa apontam a precariedade no sistema de coleta de esgoto e, ainda, as doenças de veiculação hídrica como, diarreias e verminoses. O reator UASB experimental apresentou resultados de remoção total de DQO e de DBO de 61% e 67%, podendo ser, portanto, uma alternativa viável e eficiente para o tratamento do esgoto.