bluuebunny/crossref_metadata_2025_split huggingface dataset:
Tunisian Maturity-Onset Diabetes of the Young: A Short Review and a New Molecular and Clinical Investigation
Authors: Moalla Mariam , Safi Wajdi , Babiker Mansour Maab , Hadj Kacem Mohamed , Mahfood Mona , Abid Mohamed , Kammoun Thouraya , Hachicha Mongia , Mnif-Feki Mouna , Hadj Kacem Faten , Hadj Kacem Hassen 
Published: July 2021

Introduction/AimsMaturity-Onset Diabetes of the Young (MODY) is a monogenic non-autoimmune diabetes with 14 different genetic forms. MODY-related mutations are rarely found in the Tunisian population. Here, we explored MODY related genes sequences among seventeen unrelated Tunisian probands qualifying the MODY clinical criteria.Materials and MethodsThe GCK and HNF1A genes were systematically analyzed by direct sequencing in all probands. Then, clinical exome sequencing of 4,813 genes was performed on three unrelated patients. Among them, 130 genes have been reported to be involved in the regulation of glucose metabolism, β-cell development, differentiation and function. All identified variants were analyzed according to their frequencies in the GnomAD database and validated by direct sequencing.ResultsWe identified the previously reported GCK mutation (rs1085307455) in one patient. The clinical features of the MODY2 proband were similar to previous reports. In this study, we revealed rare and novel alterations in GCK (rs780806456) and ABCC8 (rs201499958) genes with uncertain significance. We also found two likely benign alterations in HNF1A (rs1800574) and KLF11 (rs35927125) genes with minor allele frequencies similar to those depicted in public databases. No pathogenic variants have been identified through clinical exome analysis.ConclusionsThe most appropriate patients were selected, following a strict clinical screening approach, for genetic testing. However, the known MODY1-13 genes could not explain most of the Tunisian MODY cases, suggesting the involvement of unidentified genes in the majority of Tunisian affected families.
In Situ Pre-Treatment of Vascularized Composite Allografts With a Targeted Complement Inhibitor Protects Against Brain Death and Ischemia Reperfusion Induced Injuries
Authors: Lei Biao , Sleiman M. Mahdi , Cheng Qi , Tu Zhenxiao , Zhu Peng , Goddard Martin , Martins Paulo N. , Langerude Logan , Nadig Satish , Tomlinson Stephen , Atkinson Carl 
Published: July 2021

IntroductionDonor brain death (BD) is an unavoidable component of vascularized composite allograft (VCA) transplantation and a key contributor to ischemia-reperfusion injury (IRI). Complement is activated and deposited within solid organ grafts as a consequence of BD and has been shown to exacerbate IRI, although the role of BD and complement in VCA and the role it plays in IRI and VCA rejection has not been studied.MethodsBD was induced in Balb/c donors, and the VCA perfused prior to graft procurement with UW solution supplemented with or without CR2-Crry, a C3 convertase complement inhibitor that binds at sites of complement activation, such as that induced on the endothelium by induction of BD. Following perfusion, donor VCAs were cold stored for 6 hours before transplantation into C57BL/6 recipients. Donor VCAs from living donors (LD) were also procured and stored. Analyses included CR2-Crry graft binding, complement activation, toxicity, injury/inflammation, graft gene expression and survival.ResultsCompared to LD VCAs, BD donor VCAs had exacerbated IRI and rejected earlier. Following pretransplant in-situ perfusion of the donor graft, CR2-Crry bound within the graft and was retained post-transplantation. CR2-Crry treatment significantly reduced complement deposition, inflammation and IRI as compared to vehicle-treated BD donors. Treatment of BD donor VCAs with CR2-Crry led to an injury profile not dissimilar to that seen in recipients of LD VCAs.ConclusionPre-coating a VCA with CR2-Crry in a clinically relevant treatment paradigm provides localized, and therefore minimally immunosuppressive, protection from the complement-mediated effects of BD induced exacerbated IRI.
Psychedelics for Brain Injury: A Mini-Review
Authors: Khan Mia , Carter Gregory T. , Aggarwal Sunil K. , Holland Julie 
Published: July 2021

Objective: Stroke and traumatic brain injury (TBI) are among the leading causes of disability. Even after engaging in rehabilitation, nearly half of patients with severe TBI requiring hospitalization are left with major disability. Despite decades of investigation, pharmacologic treatment of brain injury is still a field in its infancy. Recent clinical trials have begun into the use of psychedelic therapeutics for treatment of brain injury. This brief review aims to summarize the current state of the science's relevance to neurorehabilitation, and may act as a resource for those seeking to understand the precedence for these ongoing clinical trials.Methods: Narrative mini-review of studies published related to psychedelic therapeutics and brain injury.Results: Recent in vitro, in vivo, and case report studies suggest psychedelic pharmacotherapies may influence the future of brain injury treatment through modulation of neuroinflammation, hippocampal neurogenesis, neuroplasticity, and brain complexity.Conclusions: Historical data on the safety of some of these substances could serve in effect as phase 0 and phase I studies. Further phase II trials will illuminate how these drugs may treat brain injury, particularly TBI and reperfusion injury from stroke.
Updated Database and Trends of Declared Low- and No-Calorie Sweeteners From Foods and Beverages Marketed in Spain
Authors: Samaniego-Vaesken Mª de Lourdes , González-Fernández Beatriz , Partearroyo Teresa , Urrialde Rafael , Varela-Moreiras Gregorio 
Published: July 2021

Background: The past few years have witnessed an increase in the availability of food products containing one or more low- and no-calorie sweeteners (LNCS) in the Spanish market, mostly due to the new massive reformulation plan. However, these are not included in food composition tables or databases, and, therefore, assessment of their intake among the population is complex. This study aims to update a database including commercialized foods and beverages.Method: A systematic search of ingredients information from the different food and beverage categories was undertaken during 2019 by recording the availability and type of LNCS declared in the information of the product from labels and online shopping platforms of retailers from Spain to update a previous food composition database compiled in 2017.Results: A total of 1,238 products were identified. The major groups were sugar and sweets (24%), non-alcoholic beverages (21%), cereals and grains (19%), and milk and dairy products (14%) accounting for &gt;70% of total products. The mainly declared LNCS were sorbitol (19.5%), sucralose (19.5%), and acesulfame K (19.2%).Conclusion: There is a wide variety of products that include LNCS as a main ingredient with higher availability than when compared with the results of database of 2017, consequently, it might be expected that LNCS are commonly consumed at present in the Spanish diet.
Clinical Significance of Metastasis or Micrometastasis to the Lymph Node Along the Superior Mesenteric Vein in Gastric Carcinoma: A Retrospective Analysis
Authors: Xu Xing , Zheng Guoliang , Zhang Tao , Zhao Yan , Zheng Zhichao 
Published: July 2021

BackgroundThe validity of lymphadenectomy of the lymph node along the superior mesenteric vein (LN14v) in gastric cancer remains controversial. The study investigated the characteristics and prognosis of gastric cancer with metastasis or micrometastasis to LN14v.MethodsA retrospective study of 626 patients undergoing radical gastrectomy in our center from January 2003 to December 2015 was analyzed. In total, 303 patients had lymphadenectomy of LN14v, and lymph node micrometastasis was evaluated by immunohistochemical staining for cytokeratin nodes CK8/18. A logistic regression model was applied to confirm the predictive factors of micrometastasis. Survival analysis was performed to evaluate the effect of micrometastasis or metastasis on prognosis.ResultsThe metastatic rate of the LN14v lymph node was 15.8%, and the micrometastatic rate was 3.3%. Multivariate analysis showed site, Borrmann classification, postoperative lymph node metastasis (pN), and metastasis in LN6 and LN9 were predictive factors for LN14v micrometastasis or metastasis (P &lt; 0.05). The 5-year survival rate in the positive group (LN14v micrometastasis or metastasis) was 12.4%. The prognosis of patients without LN14v lymph node micrometastasis was better than that of the positive group, whereas the difference between group of LN14v micrometastasis and LN14v metastasis was not obvious. In matched analysis, patients with stage III gastric cancer L/M area, pN2-3, and LN6(+) who underwent lymphadenectomy of LN14v had better survival than those without lymphadenectomy of LN14v.ConclusionLymph node micrometastasis may provide accurate prognostic information for patients with gastric cancer. Moreover, lymphadenectomy of LN14v might improve the survival of patients with stage III gastric cancer of L/M area, pN2-3, and LN6(+).
Adapting Blood DNA Methylation Aging Clocks for Use in Saliva Samples With Cell-type Deconvolution
Authors: Galkin Fedor , Kochetov Kirill , Mamoshina Polina , Zhavoronkov Alex 
Published: July 2021

DeepMAge is a deep-learning DNA methylation aging clock that measures the organismal pace of aging with the information from human epigenetic profiles. In blood samples, DeepMAge can predict chronological age within a 2.8 years error margin, but in saliva samples, its performance is drastically reduced since aging clocks are restricted by the training set domain. However, saliva is an attractive fluid for genomic studies due to its availability, compared to other tissues, including blood. In this article, we display how cell type deconvolution and elastic net can be used to expand the domain of deep aging clocks to other tissues. Using our approach, DeepMAge’s error in saliva samples was reduced from 20.9 to 4.7 years with no retraining.
Influence of Interactions between Nitrogen, Phosphorus Supply and Epichloёbromicola on Growth of Wild Barley (Hordeum brevisubulatum)
Authors: Lang Mingxiao , Zhou Jingle , Chen Taixiang , Chen Zhenjiang , Malik Kamran , Li Chunjie 
Published: July 2021

Epichloë endophytes are biotrophic fungi that establish mutualistic symbiotic relationship with grasses and affect performance of the host under different environments. Wild barley (Hordeum brevisubulatum) is an important forage grass and often infected by Epichloë bromicola, thus showing tolerances to stresses. Since the plant growth correlates with both microbial infection and nutrient stoichiometry, this study was performed to investigate whether the function of Epichloë bromicola endophyte to improve host growth depend upon the nitrogen (N), phosphorus (P) fertilization. Epichloë-infected (E+) and Epichloë-free (E−) wild barley plants were subjected to nine types of mixed N (0.2 mM, 3 mM, 15 mM) and P (0.01 mM, 0.1 mM, 1.5 mM) levels treatments for 90 d to collect plant samples and determine multiple related indexes. We found that E. bromicola and N, P additions positively affected seed germination. Further, E. bromicola significantly enhanced chlorophyll content and root metabolic activity under N-deficiency, and meanwhile, might alter allocation of photosynthate under different conditions. The contents of N, P and stoichiometry of C:N:P of E+ plants were significantly higher than that of E− under nutrient deficiency, but contrary results were observed under adequate nutrients. Therefore, we propose that the growth-promoting ability of E. bromicola is closely correlated with N and P additional levels. Under low N, P additions, positive roles of endophyte are significant as opposed to negative roles under high N, P additions.
Taxonomy and Physiology of Oxyrrhis marina and Oxyrrhis maritima in Korean Waters
Authors: Jung Min Kyoung , Yin Tae Yeon , Moon Seung Joo , Park Jaeyeon , Yoon Eun Young 
Published: July 2021

The genus Oxyrrhis is a heterotrophic dinoflagellate found in diverse marine environments. Oxyrrhis spp. have received attention owing to their ecological and industrial importance, high lipid contents, and docosahexaenoic acid formation. To the best of our knowledge, contrary to O. marina, ecophysiological characterization studies on O. maritima have not yet been reported. Therefore, we investigated the taxonomy and ecophysiology of four strains of O. marina from coastal waters and two strains of O. maritima from the littoral tidepool waters of Korea. Based on phylogenetic trees constructed using internal transcribed spacer ribosomal DNA (ITS rDNA) and SSU rDNA of dinoflagellates, the clade of all four O. marina strains was divergent from that of the two O. maritima strains. We measured the growth rates of both species at various water temperatures (10–36 °C), salinities (5–90), and light intensities (0–100 µE·m−2·s−1). The lowest (O. marina and O. maritima: 10 °C) and highest temperatures (O. marina: <35 °C, O. maritima: >35 °C) revealed that O. maritima has more tolerance to high salinity. This study provides a basis for understanding the ecophysiology of O. marina and O. maritima and their population dynamics in marine ecosystems.
Migração intrapélvica do parafuso cefálico de uma haste tipo gama: relato de um caso desafiador
Authors: Gonçalves Schroder e Souza Bruno , David Oliveira Cler , Vieira Caires Nathália , Álaf Lucinda de Sá Leonardo , Chartuni Teixeira Cury Gabriel 
Published: July 2021

Introdução: A migração intrapélvica do parafuso cefálico de hastes cefalomedulares é uma complicação rara das fraturas transtrocantéricas e está associada a alta morbi-mortalidade. Objetivo: Relatar um novo caso de migração intrapélvica do parafuso cefálico com intuito de buscar soluções para essa complicação. Relato de Caso: Apresentamos um caso de migração intrapélvica do parafuso cefálico ocorrido no pós-operatório de seis semanas de um paciente de 77 anos com fratura transtrocantérica tratado com haste cefalomedular (tipo gama) e relatos de múltiplas quedas após a cirurgia inicial, discutimos os aspectos relacionados as possíveis causas desta complicação, ao planejamento e execução do tratamento, bem como ao prognostico desta lesão desafiadora. Conclusão: Percebemos que ainda é preciso ampliar o debate em busca da elucidação completa da gênese dessa complicação, uma vez que esta ainda permanece obscura.
Modeling changes in internal pressure during the solidification of hydrous granitic intrusives: Implications for the crystallization of miarolitic pegmatites
Authors: Jacques Issac J. , Anderson Alan J. 
Published: July 2021

ABSTRACT
Volatile exsolution is widely recognized as an important trigger for eruptions from shallow magma reservoirs, but relatively few studies quantify the effects of exsolution on internal pressure within deeper-seated intrusive bodies. We present a model to predict internal pressure changes during the crystallization of a haplogranite melt containing 3 and 5 mass % H2O and with an emplacement pressure of 200 MPa. Mass and volume relations between phases are used to determine internal pressure assuming a closed, constant-volume system. The results indicate that initial crystallization of alkali feldspar and quartz causes a decrease in pressure prior to the exsolution of an aqueous fluid from the residual melt (i.e., resurgent boiling). Further crystallization toward the core of the body in the presence of a separate volatile phase results in a sharp increase in internal pressure. Our model shows that in closed, isochoric systems, the crystallization of the H2O-saturated melt will generate internal pressures that greatly exceed emplacement pressures typical of miarolitic pegmatites. Extreme overpressure modifies the physical and chemical properties of the residual melt and coexisting aqueous fluid, which in turn influences crystallization kinetics and the development of primary textures. Primary melt and fluid inclusions in pocket minerals thus likely represent samples trapped at various pressures in a rapidly evolving melt–fluid system. In most pegmatites, increasing fluid pressure and the formation of large pockets is regulated by the permeability and tensile strength of the enclosing rock. This explains why many miarolitic pegmatites occur within rigid host rocks such as granite, gabbro, and gneiss.
Implementasi Sekolah Ramah Anak Di SDN Lempuyangwangi Kota Yogyakarta
Authors: Utami Tri , Kusumawiranti Retno , Mali Matheus Gratiano 
Published: July 2021

Penelitian dilatarbelakangi oleh keprihatinan dunia pendidikan terhadap berbagai kasus yang kerap terjadi di sekolah. Dari data KPAI sepanjang Januari hingga April 2019 menyebutkan, pelanggaran hak anak masih terjadi dan didominasi perundungan, yaitu berupa kekerasan fisik, kekerasan psikis, dan kekerasan seksual, dan anak menjadi korban kebijakan. Sekolah Ramah Anak merupakan salah satu kebijakan yang dilakukan sebagai upaya pencegahan dalam mengatasi berbagai permasalahan yang melanggar hak anak terutama ketika berada di sekolah. SDN Lempuyangwangi adalah salah satu sekolah di Kota Yogyakarta yang menerapkan kebijakan Sekolah Ramah Anak (SRA) ini. Penelitian ini bertujuan untuk mendeskripsikan implementasi Program Sekolah Ramah Anak di SD Negeri Lempuyangwangi Kota Yogyakarta. Deskripsi tersebut terkait dengan implementasi Program Sekolah Ramah Anak serta faktor-faktor yang mempengaruhi dalam mengimplementasikan Program Sekolah SRA. Penelitian ini menggunakan jenis penelitian deskriptif kualitatif. Subjek dalam penelitian ini yaitu tim Sekolah Ramah Anak Kota Yogyakarta, kepala sekolah, guru, siswa, dan orang tua siswa dengan objek penelitian meliputi implementasi Program Sekolah Ramah Anak di SD Negeri Lempuyangwangi. Metode pengumpulan data yang digunakan berupa wawancara mendalam (Indepth Interview), observasi, dan dokumentasi. Teknik analisis data menggunakan model interaktif Miles dan Huberman meliputi pengumpulan data, reduksi data, penyajian data, dan verifikasi/penarikan kesimpulan. Adapun validasi data menggunakan triangulasi sumber data. Dari hasil penelitian terlihat bahwa: (1)Komitmen tertulis tentanf kebijakan tentang Sekolah Ramah Anak sudah ada; (2) metode pembelajaran berbasis PAIKEM (Pembelajaran Aktif, Inovatif, Kreatif, Efektif, dan Menyenangkan) sudah sesuai dengan prinsip SRA; (3) partisipasi anak sudah baik terlihat pada peran siswa dalam keaktifannya berekspresi, bertanya, menjawab, berargumentasi, dan memberikan interupsi; dan (4) Partisipasi orang tua, lembaga masyarakat, dunia usaha, pemangku kepentingan sudah terjalin dengan baik. Faktor yang mempengaruhi implementasi SRA di SD Negeri Lempuyangwangi meliputi: (1) Komunikasi, (2) Sumber daya manusia, sumber daya sarana prasarana dan metode yang digunakan untuk pencapaian Sekolah Ramah Anak; dan (3) Disposisi. Hasil penelitian menunjukkan bahwa program Sekolah Ramah Anak (SRA) di SDN Lempuyangwangi telah terimplikasikan dengan baik. Namun masih perlu perbaikan di bidang sarana dan prasarana serta belum terpenuhonya guru bimbingan konseling dan guru untuk ank berkebutuhan khusus (inklusi).
Pemberdayaan Masyarakat Dalam Memanfaatkan Potensi Sumber Daya Air Melalui Pengembangan Pembangkit Listrik Tenaga Picohydro di Dusun Tumba Desa Tamaila Utara
Authors: Arifin Yayu Indriati , Djafar Abdi Gunawan 
Published: July 2021

Dusun Tumba adalah salah satu dari 6 (enam) dusun yang secara administratif berada di Desa Tamaila Utara, Kecamatan Tolangohula, Kabupaten Gorontalo, Provinsi Gorontalo. Secara astronomis berada pada koordinat N 0º49’23.3” E 122º32’08.5”. Metode pelaksanaan program yang dilakukan mahasiswa KKN GEF-SGP Energi Terbarukan terbagi menjadi enam program yang bermanfaat untuk pemberdayaan di lingkungan masyarakat Dusun Tumba. Program Pemberdayaan Masyarakat Dalam Memanfaatkan Potensi Sumber Daya Air Melalui Pengembangan Pembangkit Listrik Tenaga Picohydro di Dusun Tumba Desa Tamaila Utara yakni program yang dilaksanakan berupa Pendataan Jumlah Pemakaian Energi dan Maintenance Pembangkit Listrik Tenaga Pycohidro, Pemetaan Daerah Tangkapan Air, Konsesi HTI, Pelaksanaan Produksi Film Dokumenter, serta Program Pendidikan dan Infrastruktur KKN GEF-SGP Energi Terbarukan berjalan dengan baik. Program ini diharapkan bermanfaat bagi pemberdayaan masyarakat sebagai penunjang perekonomian, pertanian, pendidikan di Dusun Tumba
Strategi Program Kampung Iklim berbasis bottom up participative dalam mendorong pencapaian target SDGs Di Desa Hutadaa
Authors: Yunginger Raghel , Dako Amirudin 
Published: July 2021

Kondisi geografis Desa Hutadaa yang masih relatif dekat dengan pusat Kabupaten Gorontalo seharusnya mendapat perhatian penting untuk menjadi salah satu desa yang dapat mendukung perekonomian yang berbasis lingkungan. Bahkan menariknya, desa ini berbatasan langsung dengan perairan Danau Limboto sebagai asset sumber daya alam sekaligus sebagai icon Provinsi Gorontalo. Kehadiran Danau Limboto ini sangat potensial untuk menumbuhkan ekonomi masyarakat yang dapat digerakkan pada pada sektor perikanan, pariwisata, dan juga sektor perekonomian. Namun ternyata banyak masalah yang ditemukan di desa ini terutama rendahnya pertumbuhan ekonomi, tingginya angka stunting, DBD, kerusakan lingkungan yang tandai dengan kejadian banjir maupun kekeringan serta tumpukan sampah. Berdasarkan permasalahan yang ditemukan di Desa Hutadaa, maka solusi yang telah dilaksanakan adalah strategi ProKlim berbasis partisipasi masyarakat yang sinergi dengan pencapaian SDGs terutama goals 3 (kesehatan yang baik dan kesejahteraa), 6 (air bersih dan sanitasi), dan 13 (penanganan perubahan iklim). Strategi ProKlim di Desa Hutadaa ini telah dirintis melalui program Kuliah Kerja Nyata (KKN) oleh mahasiswa Universitas Negeri Gorontalo selama 45 hari dengan harapan dapat dilakukan secara berkelanjutan. Adapun luaran yang telah dicapai dalam program pengabdian ini adalah inovasi pembuatan biopori, penghijauan dan pemanfaatan pekarangan, pembentukan kelompok usaha Bank Sampah, pembuatan Pupuk Kompos dan pupuk organik cair, program posyandu remaja, pembuatan spot foto untuk desa wisata, pembuatan peta perencanaan aliran drainase, peta adminsitrasi Desa Hutadaa, dan juga peta tanggap bencana alam. Namun hasil program pengabdian ini masih membutuhkan program KKN lagi untuk melanjutkan dan mengembangkan luaran yang telah dihasilkan
Pentingnya Penguatan Ekonomi Masyarakat Pada Masa Pandemi Covid 19 Dalam Meningkatan Manajemen Usaha Masyarakat Pengrajin Kue Berbahan Dasar Jagung
Authors: Abdussamad Zuchri , Tahir Arifin 
Published: July 2021

Permasalahan berantai yang sangat mengganjal pada masa pandemic Corona 19 adalah keterbatasan pengetahuan/keterampilan dan teknologi yang bisa diakses dalam hal teknologi pengolahan berbagai produk tanaman menjadi makanan ringan atau cemilan. pengemasan, pelabelan juga mutu dan hiegine, sehingga belum mampu menembus segmen pasar, ini sangat mempengaruhi ekonomi masyarakat. Permintaan konsumen dapat terpenuhi apabila tingkat produksi tetap kontinyu dan mutu olahan dapat terjaga. Aspek inilah yang masih menjadi permasalahan mendasar oleh kelompok masyarakat tentang teknologi pengembangan produk tanaman jagung atau tumbuhan lainnya. Penyelesaian permasalahan ini dapat ditempuh antara lain melalui pengembangan keterampilan dan manajemen usaha bagi masyarakat yang tergabung pada kelompok Dasawisma di Desa Bulontio Barat Kecamatan Sumalata Timur Gorontalo Utara dalam meningkatkan ekonomi kelompok masyarakat dengan memberikan pendampingan pemberdayaan ilmu dan teknologi tentang pengembangan produk tanaman jagung dan tanaman lainnya supaya lebih baik dan menghasilkan banyak pilihan produk. Berangkat dari permasalahan mitra, maka melalui program pengabdian masyarakat, bersama-sama dengan mahasiswa telah melaksanakan program KKN Tematik dalam bentuk pendampingan, penyuluhan dan pelatihan melalui peningkatan manajemen usaha bagi masyarakat/kelompok Dasawisma di Bulontio Barat Kecamatan Sumalata Timur Gorontalo Utara. Demikian halnya Proses pembelajaran dan pemberdayaan yang diperankan oleh mahasiswa dengan menggunakan metode pendampingan.