bluuebunny/crossref_metadata_2025_split huggingface dataset:
Inhibition of miR-665 alleviates lipopolysaccharide-induced inflammation via up-regulation of SOCS7 in chondrogenic ATDC5 cells
Authors: Ning Qiaoyi , He Yiting , Ma Wukai , Tang Fang , Huang Ying , Yao Xueming 
Published: January 2021

Purpose: To examine the effect and mechanism of action of miR-665 in osteoarthritis.Methods: An in vitro inflammatory injury model of osteoarthritis was established using chondrogenic ATDC5 cells with lipopolysaccharide (LPS) treatment. The expression levels of inflammatory cytokines were determined by enzyme-linked immunosorbent assays (ELISAs) and by quantitative real-time polymerase chain reaction (qRT-PCR). A binding target for miR-665 was predicted using TargetScan and then evaluated using a dual-luciferase reporter assay.Results: Treatment with LPS significantly up-regulated the inflammatory cytokine expressions of interleukin-1β (IL-1β), IL-6, and tumor necrosis factor-alpha (TNF-α), in ATDC5 cells (p < 0.01), and the expression of miRNA-665 was significantly increased in LPS-treated ATDC5 cells (p < 0.01).Knockdown of miR-665 down-regulated the expression levels of these inflammatory cytokines. Suppressor of cytokine signaling 7 (SOCS7) was identified as a target of miR-665. Data from qRT-PCR and western-blot analyses indicated that SOCS7 expression was promoted by miR-665 inhibition and inhibited by miR-665 over-expression. LPS treatment significantly decreased the expression of SOCS7 protein in ATDC5 cells (p < 0.01), and over-expression of SOCS7 attenuated the LPS-stimulated inflammatory injury. In addition, over-expression of miR-655 enhanced the inflammatory injury and reversed the protective effect of SOCS7 against LPS-stimulated inflammation.Conclusion: Inhibition of miR-665 alleviated LPS-stimulated inflammatory injury in ATDC5 cells via the up-regulation of SOCS7, suggesting a potential therapeutic target for osteoarthritis.
Keywords: MiR-665, Lipopolysaccharide, Inflammation, SOCS7, Chondrogenic, ATDC5
Effects of different extracts of Cremastra appendiculata (D. Don) Makino Cremastra appendiculata (D. Don) Makino on apoptosis of A549 cells
Authors: Zhang Haifeng , Zhang Ziying , Du Hua , Su Xiaoli , Li Xiaorong , Shao Junjie , Qiao Pengfei 
Published: January 2021

Purpose: To investigate the effect of different extracts of Cremastra appendiculata (D. Don) Makino onapoptosis of A549 cells, and the underlying mechanism.Methods: The contents of colchicine in ethyl acetate and n-butanol extracts of Cremastra appendiculata(D. Don) Makino were determined using high performance liquid chromatography (HPLC). Lung cancerA549 cells cultured in vitro were divided into blank control, standard colchicine and Cremastra appendiculata (D. Don) Makino extract groups. The effect of different extract concentrations on proliferation of the cells was determined using methyl thiazolyl diphenyl-tetrazolium (MTT) assay, while apoptosis of A549 cells induced by the extracts was evaluated by flow cytometry (FC).Results: Compared with the standard colchicine group, there was no colchicine in the n-butanol and ethyl acetate extracts of Cremastra appendiculata. Results from MTT assay showed that the extract inhibited the proliferation of A549 cells (p < 0.05). Flow cytometry results showed that ethyl acetate extract significantly enhanced apoptosis in A549 cells (p < 0.05). However, n-butanol extract had no significant effect on the apoptosis of A549 cells (p < 0.05).Conclusion: The ethyl acetate extract of Cremastra appendiculata (D. Don) Makino induces apoptosis in lung cancer A549 cells. Therefore, there is a need for further research and development of antitumor drugs from the extract of Cremastra appendiculata (D. Don) Makino.
Keywords: Cremastra appendiculata (D. Don) Makino, Colchicine, A549 cells, Apoptosis
Esomeprazole improves the acidic microenvironment of epithelial ovarian cancer by inhibiting the expression of VATPase
Authors: Chi Yasong , Yue Ruiqin , Lv Yanru , Liao Wei , Yin Ruchang 
Published: January 2021

Purpose: To determine the effect of esomeprazole on apoptosis of ovarian cancer cells and their sensitivity to paclitaxel, and the underlying mechanism.Methods: Human ovarian paclitaxel-resistant cancer cells were cultured in vitro, and treated with esomeprazole at doses of 50, 100 and 250 mol/L. Cell proliferation was determined using MTT assay. Paclitaxel-resistant cells were divided into control group, esomeprazole group, paclitaxel group, and esomeprazole + taxol group. Western blot was employed for the assay of protein levels of bcl-2, Bcl-xl, P-gp and V-ATPase, while BCECF-AM method was employed to determine changes in intracellular pH.Results: Esomeprazole significantly inhibited the proliferation of paclitaxel-resistant cells in a dosedependent manner. The half-maximal inhibitory concentration (IC50) value of esomeprazole + paclitaxel was significantly low, when compared with those of the other treatments (p < 0.05). Apoptosis was significantly higher in esomeprazole + paclitaxel group than in any other treatment group (p < 0.05). The expressions of Bcl-2 and P-gp in esomeprazole + paclitaxel group decreased significantly, relative to the corresponding values for other groups, while protein expression of bcl-xl was markedly increased. The intracellular pH value of esomeprazole + paclitaxel group was significantly lower than those for other treatment groups (p < 0.05).Conclusion: Esomeprazole improves the acidic microenvironment of epithelial ovarian cancer by inhibiting the expression of V-ATPase, and restores the sensitivity of ovarian cancer cells to paclitaxel by inhibiting their proliferation and apoptosis. This revelation may explain patients’ resistance topaclitaxel.
Keywords: Esomeprazole, V-ATPase, Apoptosis, Ovarian cancer, Taxol, Sensitivity
Quinolinone inhibits proliferation of gastric cancer cells and induces their apoptosis via down-regulation of the expression of pro-oncogene c-Myc
Authors: Ma Jing , Chen Xinrui , Xu Baoli , Liu Wenguang 
Published: January 2021

Purpose: To determine the anti-proliferative potential of quinolinone against gastric cancer cells, and the underlying mechanism of action.Methods: Quinolinone-mediated proliferative changes were measured using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay, while its effect on apoptosis was determined by flow cytometry. Transwell and wound healing assays were used for the determination of the effect of quinolinone on cell invasion and migration. The effect of quinolinone on protein expression levels were assayed with western blotting.Results: Quinolinone caused reduction in gastric cancer cell viability, but it had no effect on normal (GES-1) cells. Treatment with 8 μM quinolinone reduced the viability of SNU-5 and SGC-7901 cells to 32 and 27 %, respectively. Moreover, 8 μM quinolinone induced 67.90 and 71.54 % apoptosis in SNU-5 and SGC-7901 cells, respectively. Quinolinone significantly increased the population of cells in G1 phase, and suppressed migration potential (p < 0.05). Furthermore, in quinolinone-treated cells, the expression levels of p-PI3K, c-Myc and p-AKT were much lower than those in untreated cells (p < 0.05). Quinolinone also downregulated the expressions of MMP-2 and MMP-9, while it upregulated p21 expression in SNU-5 and SGC-7901 cells.Conclusion: Quinolinone suppresses the growth of SNU-5 and SGC-7901 gastric cancer cells via cell cycle arrest, induction of apoptosis and downregulation of the expressions of c-Myc and metalloproteinases. Thus, quinolinone may be developed as a potential drug candidate for the treatment of gastric cancer.
Keywords: Gastric cancer, Apoptosis, Metalloproteinases, Phosphorylation
AVALIAÇÃO CLÍNICA PARA DIAGNÓSTICO DE ENFERMAGEM DE RETENÇÃO URINÁRIA: CONSTRUÇÃO E VALIDAÇÃO DE PROTOCOLO
Authors: Jorge Beatriz Maria , Martins José Carlos Amado , Napoleão Anamaria Alves , Almeida Rodrigo Guimarães dos Santos , Mazzo Alessandra 
Published: January 2021

Objetivo: Construir e validar protocolo de avaliação clínica para o diagnóstico de enfermagem de retenção urinária em pacientes adultos. Métodos: Pesquisa metodológica desenvolvida em duas etapas: construção do protocolo e validação de conteúdo e aparência por meio dos aspectos: objetivos, conteúdo, linguagem, relevância, funcionalidade e usabilidade. Para validação foi utilizada técnica Delphi e considerado concordância maior que 80% entre os Juízes pelo cálculo do Índice de Validade de Conteúdo (IVC). Resultados: O protocolo foi construído na forma de documento descritivo e folheto ilustrativo contendo 28 fotografias. A primeira rodada da validação foi realizada por 71 juízes, e todos os itens alcançaram IVC ≥ 0,90 contendo algumas sugestões de modificações. A segunda rodada foi realizada com 50 juízes, o IVC foi de ≥ 0,92 nos itens avaliados. Conclusões: O protocolo construído, foi considerado válido, apropriado e pertinente ao uso, tornando-se uma ferramenta que auxilia o diagnóstico de enfermagem de retenção urinária.
Evaluasi Sistem Dapodikmen Dengan Pendekatan Utility System Pada Sekolah Menengah Atas Negeri Kota Palembang
Authors: Indriansari Raden Ayu , Antoni Darius , Atika Linda 
Published: January 2021

Dapodikmen system has not yet reached its accurately and effective utilization.. The results of observation and interview done to school operators in some high schools in Palembang show that there are different factors which determine the effectiveness of the system, for example, the content of information, ease of access and availability of the system which often has not been in accordance with the users’ needs. One approach used to evaluate the effectiveness of the system is the System Utility. The approach by Kendall & Kendall, 2007 (in Martoyo, 2015:638) review the success of the system implementation from six points of view, namely possession, goal, place, form, time and actualization utility. This approach is included in the category of system effectiveness evaluation viewed from the user's expectations. The results of this study demonstrate that of the aspect of variable utility and value of the Dapodikmen system is 4, 2636. This concludes that the availability of the system in high schools in Palembang city is effective.
INTERVIEW WITH PROFESSOR AZIZ NACIB AB'SABER
Authors: AZIZ NACIB AB’SABER (In Memoriam)  , JOÃO MAURO ARAÚJO  , ADRIANO CAPELO  , FRANCISCO ALBUQUERQUE  
Published: January 2021

Em abril de 2007, a Universidade Regional do Cariri, no momento da realização do I Simpósio de Geografia Física do Nordeste, decidiu prestar homenagem a um pesquisador ímpar: geógrafo por vocação, cidadão por coração, um dos principais estudiosos das dinâmicas do espaço brasileiro. Espaço esse que o encantou desde tenra idade em seu estado natal, São Paulo, e que o instigou através de tantas viagens Brasil adentro. Mas essa homenagem ocorria por um motivo especial - o professor Aziz Nacib Ab’Saber tinha uma vasta obra voltada para a compreensão dos “Sertões Secos”, como ele mesmo gostava de chamar o semiárido nordestino. Além da Geomorfologia, seu maior foco, os demais elementos das paisagens sertanejas não escapavam de seu olhar astuto e seus questionamentos argutos. O Domínio Morfoclimático das Depressões Interplanálticas e Intermontanas recobertas de Caatingas não foi apenas uma das grandes unidades morfoesculturais classificadas por ele, mas um lugar de fascínio, de descobertas e de grande empatia para um homem que dedicou sua vida à ciência, mas também à busca de cidadania para todos. Sem condições para a viagem até o Cariri cearense, os “Cariris Novos”, como forma de sua participação no evento que lhe outorgou o título de Doutor Honoris Causa, o professor Ab’Saber aceitou mandar uma fala para o evento. No texto a seguir, está transcrita essa conversa/entrevista, concedida por ele a um jornalista amigo meu, João Mauro Araújo, e seus colegas, Adriano Capelo e Francisco Albuquerque, em sua casa, em Cotia/SP, no mês de março de 2007. Espero que a simplicidade e a sabedoria deste grande brasileiro motivem muitas gerações de pesquisadores no caminho da ciência cidadã, como fez comigo!
Simone Cardoso RibeiroCrato/CE, Julho de 2020
Callus Induction and Phytochemical Constituents of Finger Eggplant (Solanum sp.)
Authors: SIDIK Norizzah Jaafar , DAUD Norhayati , SINANG Som Cit , OMAR Nurul Fazira 
Published: January 2021

This study examined the efficiency of callus induction on optimum concentrations of NAA (a-naphthaleneacetic acid) and BAP (6-benzyladenine) from culturing stem and leaf explants of finger eggplant (Solanum sp.) and investigated the phytochemical constituents of callus tissue. Seeds were sterilized by using 3 and 5 % Clorox solution, which gave the highest number of survival seeds (100 %) and were grown in vitro plantlets. The highest frequency of callus induction (100.00 ± 0.00 %) was obtained from stems and leaf explants that were excised from in vitro plantlets. The stem explants cultured on MS medium consisted of 1.0 mg/L NAA + 1.0 mg/L BAP, giving the maximum mean callus fresh weight (0.14 ± 0.05 g). Meanwhile, the leaf explants cultured on MS medium consisted of 0.5 mg/L NAA + 2.0 mg/L BAP, generating the maximum mean callus fresh weight (0.48 ± 0.10 g). The highest frequency of callus induction (88.00 ± 1.60 %) was obtained in solidified MS medium supplemented with 0.5 mg/L NAA + 2.0 mg/L BAP, producing the maximum mean fresh weight of callus (1.54 ± 0.27 g) and dry weight (0.90 ± 0.01 g). The results of the Phytochemical screenings of callus and dried leaf extracts indicated the presence of alkaloids, flavonoids, terpenoids, and saponins.
Does Intra-articular Tranexamic Acid Decrease Blood Loss in Total Hip Arthroplasty?
Authors: Ganesan Ganesan Ram , Vijayaraghavan Phagal Varthi 
Published: January 2021

ABSTRACT

Background
Blood is the nurturing liquid that delivers oxygen to the cells of the body and removes carbon dioxide and waste from the body. Blood is considered as liquid gold. Total hip replacement causes blood loss and in turn needs blood transfusion. The aim of the study is to find the role of intra-articular tranexamic acid (TXA) in control of blood loss in total hip replacement surgeries.

Materials and methods
A prospective study of 50 patients who underwent total hip replacement surgery. The study group and control group patients were selected through the card method. In the study group after the skin closure, 2 g of TXA was injected through the drain tube and drain tube was clamped for 1 hour and then released. Blood loss during the first and second postoperative periods from suction drain was calculated.

Results
Four hundred and fifty-five milliliters was the mean first postoperative day blood collected in the drain tube in the control group while study group had 283 mL. Similarly, the second day mean postoperative day drain was 89 mL in control group and 25 mL in study group. The mean first and second postoperative day blood loss was statistically significant between the groups.

Discussion
There was a considerable reduction of transfusions in postoperative period; hence, there was a reduced hospitalization cost and less risk of transmitted infections and transfusion reactions. There is a negligible risk of thrombosis in patients who had intravenous TXA not the intra-articular TXA.

Conclusion
Intra-articular TXA is an effective tool in controlling blood loss and reducing blood transfusions in hip replacement surgeries.
How to cite this article
Ram GG, Vijayaraghavan PV. Does Intra-articular Tranexamic Acid Decrease Blood Loss in Total Hip Arthroplasty? J Orth Joint Surg 2020;2(2):49–51.
An Observation of Posterior Tibial Slope and Metaphysio-diaphyseal Angle in Indian Population
Authors: Thirunarayanan Vasudevan , Ramprasath Dhurvas R , Amarnath Ramachandran , Arun Velmani 
Published: January 2021

ABSTRACT

Introduction
The posterior inclination of the tibial plateau relative to the longitudinal axis of tibia is referred to as the posterior tibial slope (PTS). There is paucity of data regarding PTS in Indian population. Metaphysio-diaphyseal angle (MDA) is the angle between longitudinal axis of tibia and proximal tibial metaphysis, a new entity with a possible clinical significance. This study was performed to determine the mean PTS and mean MDA to study the correlation of PTS and MDA changes with osteoarthritic degeneration in Indian population and to assess the sensitivity and specificity of PTS and MDA in detecting osteoarthritis. A descriptive, cross-sectional study design was followed.

Materials and methods
A total of 173 X-rays with true PA and lateral views were examined from 121 individuals using standardized technique. Osteoarthritis was classified based on Ahlback grading system. Posterior tibial slope was defined as the angle formed by two lines in the lateral knee radiograph. Metaphysio-diaphyseal angle is a new entity defined in this study, formed between two lines—first line is the proximal anatomical axis of the tibia and the second is the axis of the proximal tibial metaphysis. All the observations and measurements of PTS and MDA were statistically analyzed using MedCalc software.

Results
There were 121 individuals in the study with 91 osteoarthritic knees and 82 normal knees. The mean PTS among normal group is 9.69° [range 5–13° with standard deviation (SD) 1.81] and among arthritic group is 14.05° (range 10–24° with SD 2.38). The mean MDA among normal group is 19.87° (range 15–30° with SD 2.70) and among arthritic group is 25.03° (range 19–34° with SD 3.05). There is a moderate correlation between PTS and MDA (r = 0.64). Sensitivity and specificity in detecting osteoarthritis with PTS is 96.7% and 85.4% and by MDA is 90.1% and 84.7%, respectively.

Conclusion
Our study finds that native PTS is similar to that of oriental population but higher than that of Caucasians. There is moderate linear correlation between PTS and MDA. They also serve as a marker in detecting osteoarthritis with good sensitivity and specificity.
How to cite this article
Thirunarayanan V, Ramprasath DR, Amarnath R, et al. An Observation of Posterior Tibial Slope and Metaphysio-diaphyseal Angle in Indian Population. J Orth Joint Surg 2020;2(2):52–56.
Subtrochanteric Femur Fracture in Neonates: A Rare Complication after Breech Presentation
Authors: Sharma Rohit , Singla Sahil , Sharma Rajan , Bawa Ranbir S , Singh Arshpreet , Sidhu Navpreet S , Satti Satinderpal K , Dhillon Arshpreet S 
Published: January 2021

ABSTRACT
Fetus born in breech presentation are commonly delivered via cesarean section which is necessary to prevent trauma, and this extraction by operative maneuver can rarely lead to trauma and may result in femur fracture. But delivery of breech via vaginal route leads to various complications such as fractures. Femur fracture although being relatively a rare complication one of the most common fractures of the lower extremity presenting in newly born babies. In our case, 39-week mature fetus with weight 3,300 g breech presentation delivered by cesarean section presented with subtrochanteric femur fracture left side. A complete healing of the fracture, without any sequelae, was noted when the patient was simply immobilized with hip in flexion, abduction, and external rotation. The possibility of accidental injuries and traumatic complications are significantly reduced in cesarean section, especially in breech delivery when compared with vaginal delivery, but it does not completely eliminate the possible birth injuries and thus fractures of the newborn. So, clinicians must check for fractures even after cesarean section in breech presentation.
How to cite this article: Singla S, Sharma R, Sharma R, et al. Subtrochanteric Femur Fracture in Neonates: A Rare Complication after Breech Presentation. J Orth Joint Surg 2020;2(2):79–81.
Joyful Encounters: Learning to Play Well with Machines
Authors: Sandry Eleanor , Peaty Gwyneth 
Published: January 2021

Abstract
Human interactions with machines, including computers, consoles, smart devices and robots, are becoming more and more a part of everyday life. However, human–machine relations are often regarded as problematic for people, their interpersonal communication and human society more broadly. This paper provides an analysis of the characteristics that constitute ‘play’ in relation to video games and interactions with robots, arguing it is possible to position time spent on play with machines as valuable in itself, without requiring the outcomes more traditionally regarded as productive. Much of what is valuable in play can be seen to develop from embodied processes of communication within which humans and machines encounter and respond to one other. These encounters are often shaped by stories about the capabilities of machines and humans, while the interactions themselves go on to provoke new narratives. Although human– machine interaction can be theorized as ‘cyborg’ or ‘hybrid’, this paper argues that adopting the idea of the ‘assemblage’ is a better way recognize the flexibility of bringing disparate humans and machines together, whether in relation to playing a game or playing music. In rethinking the value of play, this paper emphasizes how people’s time spent interacting, whether with video games or robots, provides opportunities for them to learn more about themselves and others.
Lessons from the History of Internet Studies
Authors: Allen Matthew 
Published: January 2021

Abstract
A Keynote Presentation given at the Open Literacy Digital Games, Social Responsibility and Social Innovation Symposium in celebration of the 20-year anniversary of the founding of Internet Studies at Curtin University. Matthew is Australia’s first Professor of Internet Studies, having established the world-leading Internet Studies department at Curtin University where he worked from 1993 to 2012.
The Avant-Garde and Creativity: A Gricean Account
Authors: Carroll Noël 
Published: January 2021

Abstract
In this article, I discuss the historical imperative for art to create new forms. This imperative has become especially pronounced in the Age of the Avant-Garde. For that reason, I focus on the way in which avant-garde creativity typically proceeds. To that end, I rely on Paul Grice’s theory of communication.
Therapeutic Self-knowledge in Narrative Art
Authors: Kuplen Mojca 
Published: January 2021

Abstract
In recent years, there have been debates in aesthetics and philosophy of art on the question of whether we can acquire knowledge about the world from works of art. However, little has been written on the effects that art has on cultivating self-knowledge and self-development. While, for most of us, it seems obvious that art has these effects, little is known about how and why these effects occur. Addressing this issue is the main aim of this paper. The gist of the argument is that narrative art provides a unique opportunity to adopt a dual (first- and third-personal) perspective on the self, which is argued recently by psychologists and philosophers of mind to be necessary for obtaining the kind of self-knowledge that leads to self-development and self-change, that is, therapeutic self-knowledge.
A new spider genus (Araneae: Linyphiidae: Erigoninae) from a tropical montane cloud forest of Mexico
Authors: Ibarra-Núñez Guillermo , Chamé-Vázquez David , Maya-Morales Julieta 
Published: January 2021

A new genus and species of spider (Araneae, Linyphiidae, Erigoninae) from a tropical montane cloud forest of Mexico is described from both male and female specimens, Xim trenzado gen. et sp. nov. A phylogenetic parsimony analysis situates Xim gen. nov. as a distinct genus among the distal Erigoninae. Xim gen. nov. is sister to a clade including Ceratinopsis, Tutaibo and Sphecozone, but differs from those genera by having a high cymbium, large paracymbium, short straight embolus, male cheliceral stridulatory striae widely and evenly spaced, both sexes with a post-ocular lobe, male with two series of prolateral macrosetae on femur I, and the female by having strongly oblong, u-shaped spermathecae.