bluuebunny/crossref_metadata_2025_split huggingface dataset:
Risk factors for wound-related complications after surgical stabilization of spinal metastases with a special focus on the effect of postoperative radiation therapy
Authors: Jarvers Jan-Sven , Lange Maximilian , Schiemann Samuel , Pfränger Jan , Heyde Christoph-Eckhard , Osterhoff Georg 
Published: December 2021

Abstract
Background
Advancements in the field of oncological therapies during the last decades have led to a significantly prolonged survival of cancer patients. This has led to an increase in the incidence of spinal metastases. The purpose of this study was to assess risk factors for wound-related complications after surgical stabilization of spinal metastases with a special focus on the effect of postoperative RT and its timing.

Methods
Patients who had been treated for metastatic spine disease by surgical stabilization followed by radiotherapy between 01/2012 and 03/2019 were included and a retrospective chart review was performed.

Results
Of 604 patients who underwent stabilizing surgery for spinal metastases, 237 patients (mean age 66 years, SD 11) with a mean follow-up of 11 months (SD 7) were eligible for further analysis. Forty-one patients (17.3%) had wound-related complications, 32 of them before and 9 after beginning of the RT. Revision surgery was necessary in 26 patients (11.0%). Body weight (p = 0.021), obesity (p = 0.018), ASA > 2 (p = 0.001), and start of radiation therapy within 21 days after surgery (p = 0.047) were associated with an increased risk for wound complications. Patients with chemotherapy within 3 weeks of surgery (12%) were more likely to have a wound-related surgical revision (p = 0.031).

Conclusion
Body weight, obesity and ASA > 2 were associated with an increased risk for wound complications. Patients with chemotherapy within 3 weeks of the surgery were more likely to have a wound-related revision surgery. Patients who had begun radiation therapy within 21 days after surgery were more likely to have a wound complication compared to patients who waited longer.
Enzymatic degradation is an effective means to reduce aflatoxin contamination in maize
Authors: Schmidt Monica A. , Mao Yizhou , Opoku Joseph , Mehl Hillary L. 
Published: December 2021

Abstract
Background
Aflatoxins are carcinogenic compounds produced by certain species of Aspergillus fungi. The consumption of crops contaminated with this toxin cause serious detrimental health effects, including death, in both livestock and humans. As a consequence, both the detection and quantification of this toxin in food/feed items is tightly regulated with crops exceeding the allowed limits eliminated from food chains. Globally, this toxin causes massive agricultural and economic losses each year.

Results
In this paper we investigate the feasibility of using an aflatoxin-degrading enzyme strategy to reduce/eliminate aflatoxin loads in developing maize kernels. We used an endoplasmic reticulum (ER) targeted sub-cellular compartmentalization stabilizing strategy to accumulate an aflatoxin-degrading enzyme isolated from the edible Honey mushroom Armillariella tabescens and expressed it in embryo tissue in developing maize kernels. Three transgenic maize lines that were determined to be expressing the aflatoxin-degrading enzyme both at the RNA and protein level, were challenged with the aflatoxin-producing strain Aspergillus flavus AF13 and shown to accumulate non-detectable levels of aflatoxin at 14-days post-infection and significantly reduced levels of aflatoxin at 30-days post-infection compared to nontransgenic control Aspergillus-challenged samples.

Conclusions
The expression of an aflatoxin-degrading enzyme in developing maize kernels was shown to be an effective means to control aflatoxin in maize in pre-harvest conditions. This aflatoxin-degradation strategy could play a significant role in the enhancement of both US and global food security and sustainability.
Design of bionic active–passive hybrid-driven prosthesis based on gait analysis and simulation of compound control method
Authors: Xu Xinsheng , Xu Xiaoli , Liu Ying , Zhong Kai , Zhang Haowei 
Published: December 2021

Abstract
Purpose
The purpose of this paper is to design a prosthetic limb that is close to the motion characteristics of the normal human ankle joint.

Methods
In this study, combined with gait experiments, based on a dynamic ankle joint prosthesis, an active–passive hybrid-driven prosthesis was designed. On this basis, a real-time control algorithm based on the feedforward compensation angle outer loop is proposed. To test the effectiveness of the control method, a multi-body dynamic model and a controller model of the prosthesis were established, and a co-simulation study was carried out.

Results
A real-time control algorithm based on the feedforward compensation angle outer loop can effectively realize the gait angle curve measured in the gait test, and the error is less than the threshold. The co-simulation result and the test result have a high close rate, which reflects the real-time nature of the control algorithm. The use of parallel springs can improve the energy efficiency of the prosthetic system.

Conclusions
Based on the motion characteristics of human ankle joint prostheses, this research has completed an effective and feasible design of active and passive ankle joint prostheses. The use of control algorithms improves the controllability of the active and passive ankle joint prostheses.
Combinatorial use of environmental stresses and genetic engineering to increase ethanol titres in cyanobacteria
Authors: Andrews Fraser , Faulkner Matthew , Toogood Helen S. , Scrutton Nigel S. 
Published: December 2021

AbstractCurrent industrial bioethanol production by yeast through fermentation generates carbon dioxide. Carbon neutral bioethanol production by cyanobacteria uses biological fixation (photosynthesis) of carbon dioxide or other waste inorganic carbon sources, whilst being sustainable and renewable. The first ethanologenic cyanobacterial process was developed over two decades ago using Synechococcus elongatus PCC 7942, by incorporating the recombinant pdc and adh genes from Zymomonas mobilis. Further engineering has increased bioethanol titres 24-fold, yet current levels are far below what is required for industrial application. At the heart of the problem is that the rate of carbon fixation cannot be drastically accelerated and carbon partitioning towards bioethanol production impacts on cell fitness. Key progress has been achieved by increasing the precursor pyruvate levels intracellularly, upregulating synthetic genes and knocking out pathways competing for pyruvate. Studies have shown that cyanobacteria accumulate high proportions of carbon reserves that are mobilised under specific environmental stresses or through pathway engineering to increase ethanol production. When used in conjunction with specific genetic knockouts, they supply significantly more carbon for ethanol production. This review will discuss the progress in generating ethanologenic cyanobacteria through chassis engineering, and exploring the impact of environmental stresses on increasing carbon flux towards ethanol production.
Technical–tactical analysis of small combat games in male kickboxers: effects of varied number of opponents and area size
Authors: Ouergui Ibrahim , Delleli Slaheddine , Bouassida Anissa , Bouhlel Ezdine , Chaabene Helmi , Ardigò Luca Paolo , Franchini Emerson 
Published: December 2021

AbstractBackgroundTo handle the competition demands, sparring drills are used for specific technical–tactical training as well as physical–physiological conditioning in combat sports. While the effects of different area sizes and number of within-round sparring partners on physiological and perceptive responses in combats sports were examined in previous studies, technical and tactical aspects were not investigated. This study investigated the effect of different within-round sparring partners number (i.e., at a time; 1 vs. 1, 1 vs. 2, and 1 vs. 4) and area sizes (2 m × 2 m, 4 m × 4 m, and 6 m × 6 m) variation on the technical–tactical aspects of small combat games in kickboxing.MethodTwenty male kickboxers (mean ± standard deviation, age: 20.3 ± 0.9 years), regularly competing in regional and national events randomly performed nine different kickboxing combats, lasting 2 min each. All combats were video recorded and analyzed using the software Dartfish.ResultsResults showed that the total number of punches was significantly higher in 1 versus 4 compared with 1 versus 1 (p = 0.011, d = 0.83). Further, the total number of kicks was significantly higher in 1 versus 4 compared with 1 versus 1 and 1 versus 2 (p < 0.001; d = 0.99 and d = 0.83, respectively). Moreover, the total number of kick combinations was significantly higher in 1 versus 4 compared with 1 versus 1 and 1 versus 2 (p < 0.001; d = 1.05 and d = 0.95, respectively). The same outcome was significantly lower in 2 m × 2 m compared with 4 m × 4 m and 6 m × 6 m areas (p = 0.010 and d = − 0.45;p < 0.001 and d = − 0.6, respectively). The number of block-and-parry was significantly higher in 1 versus 4 compared with 1 versus 1 (p < 0.001, d = 1.45) and 1 versus 2 (p = 0.046, d = 0.61) and in 2 m × 2 m compared with 4 m × 4 m and 6 × 6 m areas (p < 0.001; d = 0.47 and d = 0.66, respectively). Backwards lean actions occurred more often in 2 m × 2 m compared with 4 m × 4 m (p = 0.009, d = 0.53) and 6 m × 6 m (p = 0.003, d = 0.60). However, the number of foot defenses was significantly lower in 2 m × 2 m compared with 6 m × 6 m (p < 0.001, d = 1.04) and 4 m × 4 m (p = 0.004, d = 0.63). Additionally, the number of clinches was significantly higher in 1 versus 1 compared with 1 versus 2 (p = 0.002, d = 0.7) and 1 versus 4 (p = 0.034, d = 0.45).ConclusionsThis study provides practical insights into how to manipulate within-round sparring partners' number and/or area size to train specific kickboxing technical–tactical fundamentals.Trial registrationThis study does not report results related to health care interventions using human participants and therefore it was not prospectively registered.
Some notes on conformable fractional Sturm–Liouville problems
Authors: Wang Wei-Chuan 
Published: December 2021

AbstractThe conformable fractional eigenvalue problem $$\begin{aligned} -D_{x}^{\alpha }D_{x}^{\alpha }y+q(x)y=\lambda \rho (x)y \end{aligned}$$
−

D
x
α

D
x
α

y
+
q
(
x
)
y
=
λ
ρ
(
x
)
y
is considered. We employ an easy and efficient method to derive its eigenvalue asymptotic expansion. On the basis of this result, we also investigate Ambarzumyan problems related to this eigenvalue problem as an application.
Bovine serum albumin protected gold nanozymes as a novel anti-cancer nanodrug for acute T-type lymphoblastic leukemia treatment via effect on the expression of anti-apoptotic genes
Authors: Vafa Ehsan , Bazargan-Lari Reza 
Published: December 2021

AbstractIn this paper, the bovine serum albumin protected gold nanozymes (BSA-Au nanozymes) were utilized as a novel nanodrug for treatment of acute T-type lymphoblastic leukemia (Jurkat) by production of excessive ROS and effect on the expression of anti-apoptotic genes. The effect of BSA-Au nanozymes on the Bcl-2 expression and survivin in the Jurkat cell line was checked. The results showed that the expression of anti-apoptotic genes was significantly reduced after treatment of the Jurkat cell line with the BSA-Au nanozymes (p-value of 0.001) as the potential nanodrug while their expression in the normal PBMC was not affected by the nanodrug. Moreover, the cytotoxic effect of the developed nanodrug on the Jurkat cell line was evaluated which illustrated that survival rate in the studied cell line reaches its minimum value (100% lethality, 0.0% survival) after treatment for 48 h. The IC50 for the nanodrug was calculated at 0.05 mM of the developed nanodrug. Overall, the BSA-Au nanozymes can be used as the nanodrug for treatment of T-type lymphoblastic leukemia via reducing the expression of anti-apoptotic genes, increasing the effect of common anticancer drugs such as Adriamycin and ara-C, and consequently increasing the survival of patients with leukemia.
Are orthodontic randomised controlled trials justified with a citation of an appropriate systematic review?
Authors: Patel Kishan , Cobourne Martyn T. , Pandis Nikolaos , Seehra Jadbinder 
Published: December 2021

Abstract
Background
A systematic review of the evidence should be undertaken to support the justification for undertaking a clinical trial. The aim of this study was to examine whether reports of orthodontic Randomised Clinical Trials (RCTs) cite prior systematic reviews (SR) to explain the rationale or justification of the trial. Study characteristics that predicated the citation of SR in the RCT report were also explored.

Material and methods
Orthodontic RCTs published between 1st January 2010 to 31st December 2020 in seven orthodontic journals were identified. All titles and abstracts were screened independently by two authors. Descriptive statistics and associations were assessed for the study characteristics. Logistic regression was used to identify predicators of SR inclusion in the trial report.

Results
301 RCTs fulfilling the eligibility criteria were assessed. 220 SRs were available of which 74.5% (N = 164) were cited, and 24.5% (N = 56) were not included but were available in the literature within 12 months of trial commencement. When a SR was not included in the introduction or no SR was available within 12 months of trial commencement, interventional studies were commonly cited. The continent of the corresponding author predicated the possibility of inclusion of a SR in the introduction (OR 0.36; 95% CI 0.18–0.71; p = 0.003).

Conclusions
A quarter of orthodontic RCTs (24.5%) included in this study did not cite a SR in the introduction section to justify the rationale of the trial when a relevant SR was available. To reduce research waste and optimal usage of resources, researchers should identify or conduct a systematic review of the evidence to support the rationale and justification of the trial.
Prevalence and associated factors of COVID-19 across Italian regions: a secondary analysis from a national survey on physiotherapists
Authors: Gambazza Simone , Bargeri Silvia , Campanini Isabella , Meroni Roberto , Turolla Andrea , Castellini Greta , Gianola Silvia ,   Scientific Technical Committee of AIFI, Gambazza Simone , Bargeri Silvia , Bertozzi Lucia , Campanini Isabella , Cattaneo Davide , Chiarotto Alessandro , Corbetta Davide , Costi Stefania , Meroni Roberto , Mezzarobba Susanna , Innocenti Tiziano , Pelosin Elisa , Petrarca Maurizio , Rossettini Giacomo , Testa Marco , Turolla Andrea , Vanti Carla , Castellini Greta , Gianola Silvia 
Published: December 2021

Abstract
Background
Coronavirus disease 2019 (COVID-19) broke out in China in December 2019 and now is a pandemic all around the world. In Italy, Northern regions were hit the hardest during the first wave. We aim to explore the prevalence and the exposure characteristics of physiotherapists (PTs) working in different Italian regions during the first wave of COVID-19.

Methods
Between April and May 2020 a structured anonymous online survey was distributed to all PTs registered in the National Professional Registry to collect prevalence data of a confirmed diagnosis of COVID-19 (i.e., nasopharyngeal swab and/or serological test). A bottom-up agglomerative nesting hierarchical clustering method was applied to identify groups of regions based on response rate. Multivariable logistic regression was used to explore personal and work-related factors associated with a confirmed diagnosis of COVID-19.

Results
A total of 15,566 PTs completed the survey (response rate 43.3%). The majority of respondents (57.7%) were from Northern regions. Considering all respondents, the number of confirmed COVID-19 cases in Northern and Central Italy, was higher compared to those in Southern Italy (6.9% vs. 1.8%, P < 0.001); focusing the analysis on respondents who underwent nasopharyngeal swab and/or serological test led to similar findings (14.1% vs. 6.4%, P < 0.001). Working in Northern and Central regions was associated with a higher risk of confirmed diagnosis of COVID-19 compared to Southern regions (OR 3.4, 95%CI 2.6 to 4.3). PTs working in Northern and Central regions were more likely to be reallocated to a different unit and changing job tasks, compared to their colleagues working in the Southern regions (10.5% vs 3.7%, P < 0.001).

Conclusions
Work-related risk factors were differently distributed between Italian regions at the time of first pandemic wave, and PTs working in the Northern and Central regions were more at risk of a confirmed diagnosis of COVID-19, especially when working in hospitals. Preventive and organizational measures should be applied to harmonize physiotherapy services in the national context.

Registration
https://osf.io/x7cha
Perceived stress, eating behavior, and overweight and obesity among urban adolescents
Authors: Roy S. K. , Jahan Khurshid , Alam Nurul , Rois Rumana , Ferdaus Ambrina , Israt Samina , Karim Md. Rizwanul 
Published: December 2021

Abstract
Background
WHO estimated 20% of adolescents (10–19 years) have mental health problems. We examined the prevalence and associated risk predictors of overweight/obesity and perceived stress using eating behaviors and physical activity among school-and-college-going urban adolescents in Bangladesh.

Methods
A cross-sectional study with a multistage sampling technique was employed to select 4609 adolescent students, aged 13–19 years, from all eight Bangladesh divisions during January–June 2019. Data were collected using a self-administered questionnaire containing Turconi Physical Activity Questionnaire (PAQ), Adolescent Stress Questionnaire (ASQ), Dutch Eating Behavior Questionnaire (DEBQ), and Anthropometric measurements. Logistic regression and different association measures assessed relationships among adolescent characteristics.

Results
The major 61.5% of adolescents were in moderate-to-extremely-severe levels of stress, 28.2% were overweight/obese, only 2.7% had a very active lifestyle, and 30.5% had a sedentary lifestyle. Perceived stress was positively and significantly correlated with eating behaviors and body mass index, whereas physical activity was significantly associated with the prevalence of overweight/obesity and high stress. The prevalence of overweight/obesity (53.8%) and high stress (52.5%) was higher in males. Adolescents’ obesity was 2.212 times more likely who had a sedentary lifestyle (95% CI 1.377–3.552), 1.13 times more likely for those who had experienced stress due to school/leisure conflict (95% CI 1.051–1.222), and 1.634 times more likely for those who were tempted by restrained eating behavior (95% CI 1.495–1.786).

Conclusion
Stress on secondary school-and-college-going students needs to be recognized, and strategies need to be developed to improve adolescents’ mental health.
Genome editing in cultured fishes
Authors: Hallerman Eric 
Published: December 2021

AbstractWith external fertilization, high fecundity, and established methods for propagation and larval rearing for cultured species, fish provide systems well suited to genome-editing procedures. While early experiments utilized zinc-finger nucleases and transcription activator-like effector nucleases (TALENs), most recent ones have used the CRISPR/Cas9 editor, and achieved rates of targeted genomic insertion well above those of classical transgenic methods, with lower frequencies of off-site integration. Genome-editing experiments with cultured fishes have focused on improving growth rate and disease resistance, achievement of reproductive confinement, and other valued traits. As reviewed here, advances in knowledge of key molecular pathways and, in some cases, favorable alterations of phenotype have been achieved. For example, loss-of-function of myostatin, a negative regulator of muscle growth, led to increased muscle mass, greater weight, and greater fillet yield in genome-edited lines of red sea bream, tiger puffer, and Nile tilapia than in their unedited counterparts. The red sea bream line become the first genome-edited animal to reach commercial production. As for all animals, wide adoption of genome-edited fishes will depend upon addressing issues of regulation, consumer acceptance, and breeding infrastructure.
Untangling the KRAS mutated lung cancer subsets and its therapeutic implications
Authors: Ritu Kulshrestha , Kumar Pawan , Singh Amit , Nupur K. , Spalgias Sonam , Mrigpuri Parul , Rajkumar  
Published: December 2021

AbstractThe Kirsten rat sarcoma virus transforming protein (KRAS) mutations (predominate in codons 12, 13, and 61) and genomically drive nearly one-third of lung carcinomas. These mutations have complex functions in tumorigenesis, and influence the tumor response to chemotherapy and tyrosine kinase inhibitors resulting in a poorer patient prognosis. Recent attempts using targeted therapies against KRAS alone have met with little success. The existence of specific subsets of lung cancer based on KRAS mutations and coexisting mutations are suggested. Their interactions need further elaboration before newer promising targeted therapies for KRAS mutant lung cancers can be used as earlier lines of therapy. We summarize the existing knowledge of KRAS mutations and their coexisting mutations that is relevant to lung cancer treatment, in this review. We elaborate on the prognostic impact of clinical and pathologic characteristics of lung cancer patients associated with KRAS mutations. We briefly review the currently available techniques for KRAS mutation detection on biopsy and cytology samples. Finally, we discuss the new therapeutic strategies for targeting KRAS-mutant non-small cell lung cancer (NSCLC). These may herald a new era in the treatment of KRASG12Cmutated NSCLC as well as be helpful to develop demographic subsets to predict targeted therapies and prognosis of lung cancer patients.
The effectiveness of telepsychiatry: thematic review
Authors: Sharma Gunjan , Devan Karrish 
Published: December 2021

Aims and methodThis review aims to clarify the evidence on the effectiveness of telepsychiatry following the COVID-19 pandemic. We conducted a literature review of three databases (Cochrane Library, PubMed and PsycINFO), using the terms virtual consultation/telepsychiatry/video consultation AND psychiatry/mental illness.ResultsWe identified 325 eligible papers and conducted a thematic analysis resulting in five themes: patient and clinical satisfaction, diagnostic reliability, outcomes, technology and professional guidance. The most significant factors linked to effectiveness of telepsychiatry were patient and clinician satisfaction and adequate technology to facilitate examination of the patient.Clinical implicationsThe consistent diagnostic reliability, satisfactory clinical outcomes and patient satisfaction linked to telepsychiatry favour its continued use once the pandemic ends. The main barrier is reluctance among clinicians and lack of professional guidance. We recommend education on the uses of telepsychiatry among clinicians, and the provision of professional guidance for its use from medical bodies and organisations.