bluuebunny/crossref_metadata_2025_split huggingface dataset:
A web-based survey of contact lens-related adverse events among the Japanese female population
Authors: Ono Koichi , Murakami Akira , Haishima Yuji 
Published: August 2021

AbstractTo assess the safety of cosmetic contact lenses and to identify other factors of contact lens (CL)-related complications for Japanese females. A web-based, cross-sectional, observational survey of complications related to CL use was performed. The frequencies of complications were compared between transparent and cosmetic CLs. Besides lens pigmentation, age, replacement schedule, total experience, daily wear time, location of purchase, stacking of CLs, CL exchange with friends, compliance to hygiene procedure, replacement of CLs at intervals longer than recommended, and CL wear overnight were considered as risk factors. Logistic regression analyses were performed to calculate the odds ratios. A total of 3803 Japanese females were analyzed. The frequency of adverse events was 33.4% (95%CI 31.3–35.4%) and 35.7% (95%CI 33.5–38.0%) for transparent and cosmetic CLs, respectively. In a multivariate model, statistically significant factors associated with complications included the following: quarterly schedule lenses, replacement at intervals longer than recommended, compliance to hygiene procedure, overnight wearing, purchase at physical shops and on the internet, and longer daily wearing time. Most of the risk-increasing behaviors are preventable. The role of public health ophthalmology is to increase awareness and to improve CL use behaviors.
Restricted differentiative capacity of Wt1-expressing peritoneal mesothelium in postnatal and adult mice
Authors: Wilm Thomas P. , Tanton Helen , Mutter Fiona , Foisor Veronica , Middlehurst Ben , Ward Kelly , Benameur Tarek , Hastie Nicholas , Wilm Bettina 
Published: August 2021

AbstractPreviously, genetic lineage tracing based on the mesothelial marker Wt1, appeared to show that peritoneal mesothelial cells have a range of differentiative capacities and are the direct progenitors of vascular smooth muscle in the intestine. However, it was not clear whether this was a temporally limited process or continued throughout postnatal life. Here, using a conditional Wt1-based genetic lineage tracing approach, we demonstrate that the postnatal and adult peritoneum covering intestine, mesentery and body wall only maintained itself and failed to contribute to other visceral tissues. Pulse-chase experiments of up to 6 months revealed that Wt1-expressing cells remained confined to the peritoneum and failed to differentiate into cellular components of blood vessels or other tissues underlying the peritoneum. Our data confirmed that the Wt1-lineage system also labelled submesothelial cells. Ablation of Wt1 in adult mice did not result in changes to the intestinal wall architecture. In the heart, we observed that Wt1-expressing cells maintained the epicardium and contributed to coronary vessels in newborn and adult mice. Our results demonstrate that Wt1-expressing cells in the peritoneum have limited differentiation capacities, and that contribution of Wt1-expressing cells to cardiac vasculature is based on organ-specific mechanisms.
Bedside microdialysis for detection of early brain injury after out-of-hospital cardiac arrest
Authors: Mölström Simon , Nielsen Troels Halfeld , Nordström Carl H. , Forsse Axel , Möller Sören , Venö Sören , Mamaev Dmitry , Tencer Tomas , Schmidt Henrik , Toft Palle 
Published: August 2021

AbstractBedside detection and early treatment of lasting cerebral ischemia may improve outcome after out-of-hospital cardiac arrest (OHCA). This feasibility study explores the possibilities to use microdialysis (MD) for continuous monitoring of cerebral energy metabolism by analyzing the draining cerebral venous blood. Eighteen comatose patients were continuously monitored with jugular bulb and radial artery (reference) MD following resuscitation. Median time from cardiac arrest to MD was 300 min (IQR 230–390) with median monitoring time 60 h (IQR 40–81). The lactate/pyruvate ratio in cerebral venous blood was increased during the first 20 h after OHCA, and significant differences in time-averaged mean MD metabolites between jugular venous and artery measurements, were documented (p < 0.02). In patients with unfavorable outcome (72%), cerebral venous lactate and pyruvate levels remained elevated during the study period. In conclusion, the study indicates that jugular bulb microdialysis (JBM) is feasible and safe. Biochemical signs of lasting ischemia and mitochondrial dysfunction are frequent and associated with unfavorable outcome. The technique may be used in comatose OHCA patients to monitor biochemical variables reflecting ongoing brain damage and support individualized treatment early after resuscitation.
Identifying organ dysfunction trajectory-based subphenotypes in critically ill patients with COVID-19
Authors: Su Chang , Xu Zhenxing , Hoffman Katherine , Goyal Parag , Safford Monika M. , Lee Jerry , Alvarez-Mulett Sergio , Gomez-Escobar Luis , Price David R. , Harrington John S. , Torres Lisa K. , Martinez Fernando J. , Campion Thomas R. , Wang Fei , Schenck Edward J. 
Published: August 2021

AbstractCOVID-19-associated respiratory failure offers the unprecedented opportunity to evaluate the differential host response to a uniform pathogenic insult. Understanding whether there are distinct subphenotypes of severe COVID-19 may offer insight into its pathophysiology. Sequential Organ Failure Assessment (SOFA) score is an objective and comprehensive measurement that measures dysfunction severity of six organ systems, i.e., cardiovascular, central nervous system, coagulation, liver, renal, and respiration. Our aim was to identify and characterize distinct subphenotypes of COVID-19 critical illness defined by the post-intubation trajectory of SOFA score. Intubated COVID-19 patients at two hospitals in New York city were leveraged as development and validation cohorts. Patients were grouped into mild, intermediate, and severe strata by their baseline post-intubation SOFA. Hierarchical agglomerative clustering was performed within each stratum to detect subphenotypes based on similarities amongst SOFA score trajectories evaluated by Dynamic Time Warping. Distinct worsening and recovering subphenotypes were identified within each stratum, which had distinct 7-day post-intubation SOFA progression trends. Patients in the worsening suphenotypes had a higher mortality than those in the recovering subphenotypes within each stratum (mild stratum, 29.7% vs. 10.3%, p = 0.033; intermediate stratum, 29.3% vs. 8.0%, p = 0.002; severe stratum, 53.7% vs. 22.2%, p < 0.001). Pathophysiologic biomarkers associated with progression were distinct at each stratum, including findings suggestive of inflammation in low baseline severity of illness versus hemophagocytic lymphohistiocytosis in higher baseline severity of illness. The findings suggest that there are clear worsening and recovering subphenotypes of COVID-19 respiratory failure after intubation, which are more predictive of outcomes than baseline severity of illness. Distinct progression biomarkers at differential baseline severity of illness suggests a heterogeneous pathobiology in the progression of COVID-19 respiratory failure.
Genome-wide analysis of HECT E3 ubiquitin ligase gene family in Solanum lycopersicum
Authors: Sharma Bhaskar , Saxena Harshita , Negi Harshita 
Published: August 2021

AbstractThe E3 ubiquitin ligases have been known to intrigue many researchers to date, due to their heterogenicity and substrate mediation for ubiquitin transfer to the protein. HECT (Homologous to the E6-AP Carboxyl Terminus) E3 ligases are spatially and temporally regulated for substrate specificity, E2 ubiquitin-conjugating enzyme interaction, and chain specificity during ubiquitylation. However, the role of the HECT E3 ubiquitin ligase in plant development and stress responses was rarely explored. We have conducted an in-silico genome-wide analysis to identify and predict the structural and functional aspects of HECT E3 ligase members in tomato. Fourteen members of HECT E3 ligases were identified and analyzed for the physicochemical parameters, phylogenetic relations, structural organizations, tissue-specific gene expression patterns, and protein interaction networks. Our comprehensive analysis revealed the HECT domain conservation throughout the gene family, close evolutionary relationship with different plant species, and active involvement of HECT E3 ubiquitin ligases in tomato plant development and stress responses. We speculate an indispensable biological significance of the HECT gene family through extensive participation in several plant cellular and molecular pathways.
Climate-smart agriculture practices influence weed density and diversity in cereal-based agri-food systems of western Indo-Gangetic plains
Authors: Jat Hanuman S. , Kumar Virender , Kakraliya Suresh K. , Abdallah Ahmed M. , Datta Ashim , Choudhary Madhu , Gathala Mahesh K. , McDonald Andrew J. , Jat Mangi L. , Sharma Parbodh C. 
Published: August 2021

AbstractClimate-smart agriculture (CSA)-based management practices are getting popular across South-Asia as an alternative to the conventional system for particular weed suppression, resources conservation and environmental quality. An 8-year study (2012–2013 to 2019–2020) was conducted to understand the shift in weed density and diversity under different CSA-based management practices called scenarios (Sc). These Sc involved: Sc1, conventional tillage (CT)-based rice–wheat system with flood irrigation (farmers’ practice); Sc2, CT-rice, zero tillage (ZT)-wheat–mungbean with flood irrigation (partial CA-based); Sc3, ZT rice–wheat–mungbean with flood irrigation (partial CSA-based rice); Sc4, ZT maize–wheat–mungbean with flood irrigation (partial CSA-based maize); Sc5, ZT rice–wheat–mungbean with subsurface drip irrigation (full CSA-based rice); and Sc6, ZT maize–wheat–mungbean with subsurface drip irrigation (full CSA-based maize). The most abundant weed species were P. minor > A. arvensis > M. indicus > C. album and were favored by farmers’ practice. However, CSA-based management practices suppressed these species and favored S. nigrum and R. dentatus and the effect of CSAPs was more evident in the long-term. Maximum total weed density was observed for Sc1, while minimum value was recorded under full CSA-based maize systems, where seven weed-species vanished, and P. minor density declined to 0.33 instead of 25.93 plant m−2 after 8-years of continuous cultivation. Full CSA-based maize–wheat system could be a promising alternative for the conveniently managed rice–wheat system in weed suppression in north-west India.
Large-scale assessment of lepidopteran soybean pests and efficacy of Cry1Ac soybean in Brazil
Authors: Horikoshi Renato J. , Dourado Patrick M. , Berger Geraldo U. , de S. Fernandes Davi , Omoto Celso , Willse Alan , Martinelli Samuel , Head Graham P. , Corrêa Alberto S. 
Published: August 2021

AbstractThe soybean technology MON 87701 × MON 89788, expressing Cry1Ac and conferring tolerance to glyphosate, has been widely adopted in Brazil since 2013. However, pest shifts or resistance evolution could reduce the benefits of this technology. To assess Cry1Ac soybean performance and understand the composition of lepidopteran pest species attacking soybeans, we implemented large-scale sampling of larvae on commercial soybean fields during the 2019 and 2020 crop seasons to compare with data collected prior to the introduction of Cry1Ac soybeans. Chrysodeixis includens was the main lepidopteran pest in non-Bt fields. More than 98% of larvae found in Cry1Ac soybean were Spodoptera spp., although the numbers of Spodoptera were similar between Cry1Ac soybean and non-Bt fields. Cry1Ac soybean provided a high level of protection against Anticarsia gemmatalis, C. includens, Chloridea virescens and Helicoverpa spp. Significant reductions in insecticide sprays for lepidopteran control in soybean were observed from 2012 to 2019. Our study showed that C. includens and A. gemmatalis continue to be primary lepidopteran pests of soybean in Brazil and that Cry1Ac soybean continues to effectively manage the target lepidopteran pests. However, there was an increase in the relative abundance of non-target Spodoptera spp. larvae in both non-Bt and Cry1Ac soybeans.
Heterogeneous mental health development during the COVID-19 pandemic in the United Kingdom
Authors: Ellwardt Lea , Präg Patrick 
Published: August 2021

AbstractThe COVID-19 pandemic and the mitigation measures by governments have upended the economic and social lives of many, leading to widespread psychological distress. We explore heterogeneity in trajectories of psychological distress during the pandemic in the United Kingdom and relate this heterogeneity to socio-demographic and health factors. We analyze nine waves of longitudinal, nationally representative survey data from the UK Household Longitudinal Study ($$N=15{,}914$$

N
=
15
,
914

), covering the period from early 2020 to mid-2021. First, latent class mixture modelling is used to identify trajectories of psychological distress. Second, associations of the trajectories with covariates are tested with multinomial logistic regressions. We find four different trajectories of distress: continuously low, temporarily elevated, repeatedly elevated, and continuously elevated distress. Nearly two fifths of the population experienced severely elevated risks of distress during the pandemic. Long-term distress was highest among younger people, women, people living without a partner, those who had no work or lost income, and those with previous health conditions or COVID-19 symptoms. Given the threat of persistent stress on health, policy measures should be sensitized to the unintended yet far-reaching consequences of non-pharmaceutical interventions.
Design and modeling of a planar graphene structure as a terahertz cyclotron radiation source
Authors: Planillo Jordan , Alves Fabio 
Published: August 2021

AbstractWith incredibly high carrier mobility and saturation velocity, graphene would be an ideal candidate for a miniaturized solid-state cyclotron radiation source. A planar semicircular graphene arc geometry was investigated for emission in the 0.5–1.5 THz range. Analytical studies, confirmed by finite element simulations, show that the emitted THz frequencies are inversely proportional to the arc radius given a fixed charge-carrier velocity. The simulations show that the desired frequency spectrum can be obtained with design radii ranging from 50 to 150 nm. Interestingly, the radiated spectrum is independent of the frequency of the stimulation of the graphene nano-arcs. The simulations also indicate that the total output power correlates well with the Larmor formulation. The device is expected to emit 1 nW/cm2, which confirms the findings of existing research in this field. Such a design could yield a scalable and cost-effective THz source.
The immune status, oxidative and epigenetic changes in tissues of turkeys fed diets with different ratios of arginine and lysine
Authors: Ognik Katarzyna , Mikulski Dariusz , Konieczka Paweł , Tykałowski Bartłomiej , Krauze Magdalena , Stępniowska Anna , Nynca Anna , Jankowski Jan 
Published: August 2021

AbstractIn the present experiment, it was assumed that the appropriate dietary ratio of arginine (Arg) to lysine (Lys) can improve the immune status and growth performance of turkeys. The aim of this study was to evaluate the effects of two inclusion rates of Arg relative to Lys in turkey diets with Lys content consistent with National Research Council (NRC) recommendations or 10% higher on the immune status of birds and indicators of protein and DNA damage due to oxidation, nitration or epigenetic changes. Another goal was to determine which dietary Arg:Lys ratio stimulates the immune response of turkeys vaccinated against Ornithobacterium rhinotracheale. The experiment was performed on 576 turkeys randomly assigned to four groups with two levels of Lys (low = NRC recommendation or high = NRC + 10%) and two levels of Arg (95% or 105% Arg relative to the content of dietary Lys). It was found that the Lys content of turkey diets should be 10% higher than that recommended by the NRC and combined with the higher Arg level (105% of Lys). Although the above Arg:Lys ratio did not improve the growth performance of birds, it stimulated their immune system and reduced protein nitration as well as protein and DNA oxidation.
Mirror neurons are modulated by grip force and reward expectation in the sensorimotor cortices (S1, M1, PMd, PMv)
Authors: Atique Md Moin Uddin , Francis Joseph Thachil 
Published: August 2021

AbstractMirror Neurons (MNs) respond similarly when primates make or observe grasping movements. Recent work indicates that reward expectation influences rostral M1 (rM1) during manual, observational, and Brain Machine Interface (BMI) reaching movements. Previous work showed MNs are modulated by subjective value. Here we expand on the above work utilizing two non-human primates (NHPs), one maleMacacaRadiata (NHP S) and one femaleMacacaMulatta (NHP P), that were trained to perform a cued reward level isometric grip-force task, where the NHPs had to apply visually cued grip-force to move and transport a virtual object. We found a population of (S1 area 1–2, rM1, PMd, PMv) units that significantly represented grip-force during manual and observational trials. We found the neural representation of visually cued force was similar during observational trials and manual trials for the same units; however, the representation was weaker during observational trials. Comparing changes in neural time lags between manual and observational tasks indicated that a subpopulation fit the standard MN definition of observational neural activity lagging the visual information. Neural activity in (S1 areas 1–2, rM1, PMd, PMv) significantly represented force and reward expectation. In summary, we present results indicating that sensorimotor cortices have MNs for visually cued force and value.
Real-time SARS-CoV-2 diagnostic and variants tracking over multiple candidates using nanopore DNA sequencing
Authors: Stüder François , Petit Jean-Louis , Engelen Stefan , Mendoza-Parra Marco Antonio 
Published: August 2021

AbstractSince December 2019, a novel coronavirus responsible for a severe acute respiratory syndrome (SARS-CoV-2) is accountable for a major pandemic situation. The emergence of the B.1.1.7 strain, as a highly transmissible variant has accelerated the world-wide interest in tracking SARS-CoV-2 variants’ occurrence. Similarly, other extremely infectious variants, were described and further others are expected to be discovered due to the long period of time on which the pandemic situation is lasting. All described SARS-CoV-2 variants present several mutations within the gene encoding the Spike protein, involved in host receptor recognition and entry into the cell. Hence, instead of sequencing the whole viral genome for variants’ tracking, herein we propose to focus on the SPIKE region to increase the number of candidate samples to screen at once; an essential aspect to accelerate diagnostics, but also variants’ emergence/progression surveillance. This proof of concept study accomplishes both at once, population-scale diagnostics and variants' tracking. This strategy relies on (1) the use of the portable MinION DNA sequencer; (2) a DNA barcoding and a SPIKE gene-centered variant’s tracking, increasing the number of candidates per assay; and (3) a real-time diagnostics and variant’s tracking monitoring thanks to our software RETIVAD. This strategy represents an optimal solution for addressing the current needs on SARS-CoV-2 progression surveillance, notably due to its affordable implementation, allowing its implantation even in remote places over the world.
Optimizing the layer thickness of sol–gel-derived TiO2 coating on polyetheretherketone
Authors: Hayashi Makoto , Shimizu Takayoshi , Imamura Masashi , Fujibayashi Shunsuke , Yamaguchi Seiji , Goto Koji , Otsuki Bungo , Kawai Toshiyuki , Okuzu Yaichiro , Matsuda Shuichi 
Published: August 2021

AbstractSol–gel-derived TiO2 coatings have been confirmed to effectively promote bone-bonding behavior on polyetheretherketone (PEEK) surfaces; however, the optimal layer thickness to maximize the osseointegration and adhesive performance has not been yet determined. In this study, we applied sol–gel-derived TiO2 coatings with different layer thicknesses (40 and 120 nm) on PEEK implants to determine the effects of layer thickness on the surface characteristics, adhesive strength, and bone bonding capabilities (including histological osseointegration). The surface analysis results of both coated implants indicated no significant differences concerning the water contact angle, layer adhesion strength, and apatite formation ability in a simulated body fluid. Additionally, the in vivo biomechanical tests revealed a higher bone-bonding strength for both coated PEEK implants (compared with that of the uncoated sample). It was thus concluded that the factor of layer thickness marginally influences the bioactive advantages attained by sol–gel-derived TiO2 coatings on PEEK surfaces, highlighting the significant versatility and clinical availability of this coating technology.
New strategies for base frame fabrication in microtia reconstruction
Authors: Li Yiyuan , Li Datao , Xu Zhicheng , Zhang Ruhong , Zhang Qun , Xu Feng , Chen Xia 
Published: August 2021

AbstractThe base frame provides a stable support for the helix, antihelix, and tragus–antitragus complex in microtia reconstruction, and this support is vital to attain a highly defined outline for a reconstructed auricle. The success of base frame sculpting depends on appropriate treatment of the cartilage, mainly the sixth and seventh costal cartilages, which may have different characteristics. The aim of this study was to demonstrate the relevant details for base frame fabrication under various scenarios. Between 2016 and 2019, a total of 352 patients with microtia underwent autologous auricular reconstruction. Concerning the different sizes and characteristics of the costal cartilage used for the base frame reconstruction, we describe the related methods for fabrication and introduce corresponding strategies for proper management. We found that 90% of the patients responded at follow-up, and 76% of them were satisfied with the cosmetically refined auricle with harmonious integrity. The elaborate design and appropriate utilization of costal cartilage for base frame sculpting is one of the most significant and fundamental processes in microtia reconstruction. It contributes to achieving a clearly defined outline of the auricle with harmonious integrity, which is as important as the other projected subunits.