bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluation of Changes in Gastric Ph and Gastrine Concentrations in Horses Subjected to Inhalatory General Anesthesia in Dorsal Decubitus
Authors: Guerrero Jesus Leonardo Suarez , Brito Pedro Henrique Salles , Ferreira Marilia Alves , Arantes Julia de Asis , Rush Elidiane , Carregaro Adriano Bonfim , Doria Renata Gebara Sampaio 
Published: November 2021

Abstract
Background: In high-performance horses, it is reported that 50 to 90% have stomach disorders, especially gastritis and gastric ulceration, which compromises both, athletic performances, and health status. This work aims to stablish changes in gastric pH and gastrin hormone in horses submitted to general inhalation anesthesia and in supine position, to determine the occurrence of duodenogastric and gastroesophageal reflux. Eight mares fasted for eight hours, subjected to inhaled anesthesia and in supine position were monitored during the anesthetic period. Gastric pH, arterial blood gases and vital signs data were recorded.Results: In the post-anesthetic period, gastric pH was evaluated for 24 h, samples were collected at one-hour intervals. In addition, blood samples were collected for gastrin hormone evaluation before the anesthetic procedure (8-hour fast), during the anesthetic recovery period, and four months after the anesthetic procedure, 90 minutes after the morning meal. Gastric pH during the anesthetic period remained within physiological values (mean value 4.52 ± 1.69), without changes between times (p > 0.05). After anesthesia, mean gastric pH values increased, remaining alkaline during the 24 h of evaluation, differences between T0 (4.88±2.38), T5 (7.08±0.89), T8 (7,43±0,22), T9(7,28±0,36), T11 (7.26±0.71), T13 (6.74±0.90), and T17 (6.94±1.04) (p < 0.05) were observed. There was an increase in gastrin hormone in the post-anesthetic period (20.15±7.65 pg/ml) compared to baseline (15.15±3.82 pg/ml) (p < 0.05). Conclusions: The results obtained demonstrate that general inhalational anesthesia and dorsal decubitus in horses, for 90 min, promotes gastric pH alkalinization for 24hours after anesthesia, possibly induced by entero-gastric reflux. Therefore, gastric mucosa protectors should be considered preventively when horses are submitted to general anesthesia and supine.
What Was the Impact of COVID-19 Pandemic on Low Back Pain? Data from the PAMPA Cohort, Brazil.
Authors: Caputo Eduardo L , Ferreira Paulo H , Feter Natan , Doring Igor R , Leite Jayne S , Alt Ricardo , Cassuriaga Júlia , Reichert Felipe F , Rombaldi Airton J , Silva Marcelo C da 
Published: November 2021

Abstract
Background: To evaluate how COVID-19 pandemic impacted low back pain (LBP) outcomes in southern Brazil. Methods: Data from the Prospective Study about Mental and Physical Health (PAMPA) Cohort were analyzed. Adults were recruited between June and July 2020 in the Rio Grande do Sul state using online-based strategies. Participants responded a self-reported, online questionnaire on LBP with two timepoints: before (retrospectively) and during COVID-19 pandemic. We assessed the presence of LBP, LBP-related activity limitation (no/yes), and LBP intensity (0 to 10 [strongest pain]). Results: From a total sample of 2,321 respondents (mean age: 37.6 ± 13.5; 75.4% women), the prevalence of LBP did not change significantly from before (74.7% [95%CI 72.3; 76.9]) to during the COVID-19 pandemic (74.2% [95%CI 71.9; 76.3]). However, an increased pain levels (β: 0.40; 95%CI 0.22; 0.58) and a higher likelihood for activity limitation due to LBP was observed during the pandemic (PR 1.14; 95%CI 1.01; 1.29). Longitudinal analyzes showed that age, gender, BMI, chronic diseases, physical activity and anxiety and depression symptoms, were associated with LBP during the COVID-19 pandemic. Conclusion: Although the prevalence of LBP did not change during the COVID-19 pandemic, LBP-induced impairment in daily activities and LBP intensity was higher during than before the pandemic. Sociodemographic and lifestyle characteristics were associated with increased LBP and related outcomes.
Health-Related Quality of Life and Associated Factors in Chinese Menstrual Migraine Patients
Authors: Luo Wenxiu , Cao Xing , Zhao Jiayu , Yang Jiaming , Cen Yu , He Jinlong , Luo Jing , Zhong Yunling , Luo Ying , Wang Xinyue , Yang Liqiu , Feng Xingyu , Pang Xiaoqing , Luo Jiaming 
Published: November 2021

Abstract
Background

Menstrual migraine is a special form of migraine with significant impact on quality of life for those women afflicted. Presently, there is no study reported on quality of life in menstrual migraine patients. The study reported here aimed to assess the health-related quality of life and identify its associated factors among Chinese menstrual migraine patients.
Methods

The cross-sectional study group consisted of 109 patients with menstrual migraine and the control group consisted of 397 female patients with non-menstrual migraine. In total, 506 patients completed questionnaires for demographic and clinical information, Self-rating Idea of Suicide Scale, Hamilton Depression Scale, Hamilton Anxiety Scale, Headache Impact Test-6, Perceived Social Support Scale, Pittsburgh Sleep Quality Index. Health-related quality of life was measured using the 36-Item Short Form Survey.
Results

Compared with non-menstrual migraine patients, five dimensions of health-related quality of life were all found to be significantly impaired in menstrual migraine patients. Headache frequency (ß=-0.218, P=0.014), the impact of headache on daily life (ß=-0.270, P=0.002), depression symptoms (ß=-0.345, P<0.001) were significantly associated with physical component summary, depression symptoms (ß= -0.379, P<0.001), social support (ß=0.270, P<0.001), suicidal ideation (ß=-0.344, P<0.001) were closely related to mental component summary.
Conclusion

Menstrual migraine patients had significantly poorer health-related quality of life in many domains than non-menstrual migraine patients. Headache frequency, the impact of headache on daily life, depression symptoms, social support and suicidal ideation were significantly associated with health-related quality of life in menstrual migraine patients.
Trial registration:

ChiCTR1800014343. Registered 01 July 2018 at Chinese Clinical Trial registry. http://www.chictr.org.cns
Lift-off reduction and patella tracking in Total Knee Arthroplasty (TKA) using a 3D printed patient-specific knee model
Authors: Mercader Alexandra , Ostner Isabella , Küppers Freya , Lueth Tim C. , Röttinger Heinz , Bigdeli Amir H. 
Published: November 2021

Abstract
The patellofemoral joint is greatly influenced by the geometry and position of the femur during knee flexion. After knee replacement surgery, the joint geometry is changed by the surface replacement and its kinematics by the orientation of the cutting planes planned by the surgeon. The technique presented in this study verifies the compatibility of the cutting method during total knee arthroplasty (TKA) with the geometry of the joint and the kinematics of knee flexion. To confirm the benefit of this method, three patient-specific knee models were printed in 3D. All models were made from the patient’s CT data and the patient’s measurement of flexion motion obtained with an infrared stereo camera. The models are operated according to three different techniques: Kinematic Alignment (KA), Measured Resection (MR) with 3 degrees of external rotation and MR with 9 degrees of external rotation. The resulting postoperative knees from the different alignment techniques are compared to the patient’s preoperative knee. The results show that the movement of the patella is modified after surgery on the model. The different alignments also lead to a variation in the behavior of the tibiofemoral joint. Based on these three examples, MR with an external orientation of the prosthesis results in a patellar movement closer to the preoperative movement. The KA method also reproduces the patella movement almost identically, but a lift-off appears at the tibiofemoral joint. The error observed for each cutting method between the pre-and postoperative patella position varies by a maximum of 5 degrees of rotation and 5 mm of translation, showing that the configuration has an overall small impact on the patellar movement. This study of three cases shows the importance of the preparation of the operation and the adjustment of the prosthesis for each specific patient using the 3D printed model.
Changes in the Density and Composition of Rhizosphere Pathogenic Fusarium and Beneficial Trichoderma Contributing to Reduced Root Rot of Intercropped Soybean
Authors: Chang Xiaoli , Xu Huiting , Yan Li , Zhu Dan , Wang Wei , Naeem Muhammd , Song Chun , Wu Xiaoling , Gong Guoshu , Chen Huabao , Zhang Min , Liu Taiguo , Chen Wanquan , Yang Wenyu 
Published: November 2021

Abstract
BackgroundThe dynamic of soil-borne disease is closely related to the rhizosphere microbial communities. Maize-soybean intercropping can suppress soybean root rot as compared to monoculture. However, it is still unknown whether rhizosphere microbial community participates in the regulation of intercropped soybean root rot.MethodsIn this study, the difference of rhizosphere Fusarium and Trichoderma community was compared between healthy or root-rotted soybean rhizosphere soil from soybean monoculture and maize-soybean intercropping, and the inhibitory effect of potential biocontrol Trichoderma against pathogenic Fusarium were examined.ResultsThe abundance of rhizosphere Fusarium was remarkably different between intercropping and monoculture, while Trichoderma was largely accumulated in healthy rhizosphere soil of intercropping rather than monoculture. Four rhizosphere Fusarium species identified were all pathogenic to soybean but displayed distinct composition and isolation proportion in the corresponding soil types. As the dominant and most aggressive species, F. oxysporum was more frequently isolated in diseased soil of monoculture. Furthermore, of three Trichoderma species identified, T. harzianum dramatically increased in the rhizosphere of intercropping rather than monoculture as compared to T. virens and T. afroharzianum. For in-vitro antagonism test, Trichoderma strains had antagonistic effects on F. oxysporum with the percentage of mycelial inhibition ranging of 50.59%-92.94%, and they displayed good mycoparasitic abilities against F. oxysporum through coiling around and entering into the hyphae, expanding along cell-cell lumen and even dissolving cell walls of target fungus.Conclusion﻿These results indicate maize-soybean intercropping significantly increase the density and composition proportion of beneficial Trichoderma to antagonist the pathogenic Fusarium species, thus contributing to the suppression of soybean root rot under intercropping.
Bupivacaine modulates the apoptosis and ferroptosis in bladder cancer via PI3K/AKT pathway
Authors: Hao Jianli , Zhang Weiqing , Huang Zeqing 
Published: November 2021

Abstract
Background
The study aimed to explore the effects of local anesthetic bupivacaine on bladder cancer cells in vivo and in vitro.
Methods
After T24 cells and 5637 cells were treated with different doses of bupivacaine (0-16 mM) for 24 h, MTT assay was used to detect the cytotoxicity, and bupivacaine (0.25, 0.5, 1 mM) was selected for subsequent experiments. Apoptosis was detected by Hoechst 33342 staining and TUNEL. The contents of Fe2+, MDA, GSH and ROS were detected by the corresponding kit. Mitochondrial membrane potential was detected by JC-1 kit. HE staining, TUNEL and immunohistochemistry were used to detect the xenografted tumors. Protein expression was detected by western blot.
Results
Bupivacaine significantly inhibited the activity of T24 cells and 5637 cells at 0.25-16 mM. Bupivacaine significantly promoted cell apoptosis with increasd concentration. bupivacaine inhibited the expression of Bcl-2 and increased the expression of Bax and cytochrome C. Moreover, bupivacaine promoted the increase of Fe2+ and ROS, and inhibited the expression of xCT and GPX4. Further results showed that bupivacaine decreased mitochondrial membrane potential, reduced GSH, and increased MDA levels. Besides, bupivacaine attenuated the phosphorylation of PI3K, Akt, and mTOR, which might be involved in the regulation of bupivacaine-induced apoptosis and ferroptosis. In addition, bupivacaine suppressed the growth of xenografted tumors, induced apoptosis and ferroptosis, and inhibited the activity of PI3K/AKT signaling pathway in xenografted tumors.
Conclusion
Bupivacaine could induce apoptosis and ferroptosis by inhibiting PI3K / Akt signaling pathway in bladder cancer cells.
Alterations in Vaginal Microbiota among Pregnant Women with COVID-19
Authors: Celik Ebru , Ozcan Gulin , Vatansever Cansel , Paerhati Erxiati , Kuskucu Mert Ahmet , Dogan Ozlem , Cekic Sebile Guler , Ergonul Onder , Gursoy Atilla , Keskin Ozlem , Can Fusun 
Published: November 2021

Abstract

Background: The maintenance of vaginal microbiota is an important factor to achieve ideal pregnancy outcomes. Coronavirus disease (COVID-19) has been shown to have potential adverse effects on pregnancy and neonatal outcomes. Pregnancy itself is a risk factor for the severity of COVID-19, with an increased risk of intensive care unit (ICU) admission, maternal morbidity, and mortality. the role of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infection in vaginal microbiome composition in pregnant women with COVID-19 has not yet been investigated. Therefore, we anticipate that COVID-19 may unfavorably affect the composition of the vaginal microbiota, resulting in adverse pregnancy outcomes. We aimed to describe the alterations of the composition of vaginal microbiota in pregnant women with COVID-19.Results: A prospective case-control study was conducted among 19 pregnant women with COVID-19 and 28 healthy controls matched according to the gestational week and age. The vaginal swabs were collected during the active phase of infection and consecutively, within a month after recovering from infection. In three patients, longitudinal samples before, in the course, and after infection were also obtained. The microbiome alterations were examined by 16S rRNA gene sequencing. We indicated that COVID-19 was associated with vaginal dysbiosis during pregnancy, which was indicated by an increased alpha diversity index. There was a significantly decrease in Firmicutes (P=0.007) and Lactobacillus (P=0.019) with an increase in Bacteroidetes (P=0.024) in the COVID-19 group. In the moderate/severe group, increased amounts of Ureaplasma and vanishing of Lactobacillus gasseri were found in women, compared to the asymptomatic or mild group (P=0.001). In longitudinal analysis, elevation of Actinobacteria with reduction of Firmicutes and Bacteroides were indicated during the active phase. Conclusions: The study revealed vaginal dysbiosis with a low abundance of Lactobacillus and an increase in Bacteroidetes in relation to SARS-CoV-2 infection. Vaginal dysbiosis in COVID-19 could be a contributing factor in pregnancy adverse outcomes. Trial registration: clinicaltrials, Registered 15 November 2019, https://www.clinicaltrials.gov/ct2/show/NCT04165252?cond=microbiota&cntry=TR&draw=3&rank=12
Age and Sex Effects across the Blood Proteome after Lonizing Radiation Exposure: Consequences for Biomarker Screening and Risk Assessment
Authors: Langen Britta , Vorontsov Egor , Spetz Johan , Swanpalmer John , Sihlbom Carina , Helou Khalil , Forssell-Aronsson Eva 
Published: November 2021

Abstract
Molecular biomarkers of ionizing radiation (IR) exposure are a promising new tool in various disciplines: they can give necessary information for adaptive treatment planning in cancer radiotherapy, enable risk projection for radiation-induced survivorship diseases, or facilitate triage and intervention in radiation hazard events. However, radiation biomarker discovery has not yet resolved the most basic features of personalized medicine: age and sex. To overcome this critical bias in biomarker identification, we quantitated age and sex effects and assessed their relevance in the radiation response across the blood proteome. We used high-throughput mass spectrometry on blood plasma collected 24 hours after 0.5 Gy total body irradiation (15 MV nominal photon energy) from male and female C57BL/6N mice at juvenile (7-weeks-old) or adult (18-weeks-old) age. We also assessed sex and strain effects using juvenile male and female BALB/c nude mice. We showed that age and sex created significant effects in the proteomic response regarding both extent and functional quality of IR-induced responses. Furthermore, we found that age and sex effects appeared non-linear and were often end-point specific. Overall, age contributed more to differences in the proteomic response than sex, most notably in immune responses, oxidative stress, and apoptotic cell death. Interestingly, sex effects were pronounced for DNA damage & repair pathways and associated cellular outcome (pro-survival vs. pro-apoptotic). Only one protein (AHSP) was identified as a potential general biomarker candidate across age and sex, while GMNN, REG3B, and SNCA indicated some response similarity across age. This low yield advocated that unisex or uniage biomarker screening approaches are not feasible. In conclusion, age- and sex-specific screening approaches should be implemented as standard protocol to ensure robustness and diagnostic power of biomarker candidates. Bias-free molecular biomarkers are a necessary progression towards personalized medicine and integral for advanced adaptive cancer radiotherapy and risk assessment.
Mettl14 Mediates the Inflammatory Response of Macrophages in Atherosclerosis Through the NF-κB/IL-6 Signaling Pathway
Authors: Zheng Yang , Li Yunqi , Ran Xianwen , Wang Di , Zheng Xianghui , Zhang Maomao , Wu Jian , Sun Yong , Yu Bo 
Published: November 2021

Abstract
The inflammatory response of macrophages has been reported to play a critical role in atherosclerosis. The inflammatory state of macrophages is modified by epigenetic reprogramming. m6A RNA methylation is an epigenetic modification of RNAs. However, little is known about the potential roles and underlying mechanisms of m6A modification in macrophage inflammation. Herein, we showed that the expression of the m6A modification “writer” Mettl14 was increased in coronary heart disease and LPS-stimulated THP-1 cells. Knockdown of Mettl14 promoted M2 polarization of macrophages, inhibited foam cell formation and decreased migration. Mechanistically, the expression of Myd88 and IL-6 was decreased in Mettl14 knockdown cells. Through m6A modification, Mettl14 regulated the stability of Myd88 mRNA. Furthermore, Myd88 affected the transcription of IL-6 via the distribution of p65 in nuclei rather than directly regulating the expression of IL-6 through m6A modification. In vivo, Mettl14 gene knockout significantly reduced the inflammatory response of macrophages and the development of atherosclerotic plaques. Taken together, our data demonstrate that Mettl14 plays a vital role in macrophage inflammation in atherosclerosis via the NF-κB/IL-6 signaling pathway, suggesting that Mettl14 may be a promising therapeutic target for the clinical treatment of atherosclerosis.
Distribution and Associated Factors of Gyrodactylus Malalai Infestation in North African Catfish, Clarias Gariepinus in Ilorin, Nigeria
Authors: Ibrahim Adeshina , Setufe Seyramsarah Blossom , Abdulwaheed Kaosarat Omolara , Adeniyi Olarinke Victoria 
Published: November 2021

Abstract
This study examined the occurrence and associated factors of Gyrodactylus malalai infestation in 30 cultured North African catfish in Ilorin, Nigeria. Four organs (gills, eyes, fin and skin) were collected from each fish (resulted into 180 organs) and observed for the existence of G. malalai. Fifty percent (50%) of the fish examined were infested with G. malalai. Males (30%) fish were more affected than the females (20%). Density, intensity and index of infestation were significantly different between males and females (p < 0.05). Fish with total length of 40.0 to 49.9 cm were significantly infested than fish with total length of 20.0 to 29.9 cm (p < 0.05) but body weight did not significantly affect the occurrence of G. malalai infestation (p > 0.05). Gills is most targeted organ of G. malalai infestation with higher percentage of infestation (8.33%), density of infestation (0.22), intensity of infestation (2.60), and index of infestation (3.25) than the eye with 2.22, 0.06, 1.57, and 0.43, respectively (p < 0.05). In conclusion, Gyrodactylus malalai infestations are high in North African catfish and could multiply rapidly and could lead to mortality if not properly managed. Furthermore, total length is identified as the predisposing factor for G. malalai infestation in fish
The Knowledge of Fermentation and Health Benefits among General Population in North-Eastern Slovenia
Authors: Pogačar Maja Šikić , Mičetić-Turk Dušanka , Fijan Sabina 
Published: November 2021

Abstract
Background

Fermented foods are staples of the human diet and fermentation process has been used by humans for thousands of years. The preparation of fermented foods was performed in the past without knowledge of the role of microorganisms involved. Nowadays, fermented foods, due to their proclaimed health benefits for consumers, are becoming increasingly popular. Our study was constructed to provide data on awareness and use of fermented foods among people in North-eastern Slovenia.
Methods

The cross-sectional study included 349 individuals (16-89 years of age). An online survey was designed to assess the participants' knowledge of fermentation, fermented foods, the consumption of fermented foods and awareness of the health benefits. Data were collected from March to June 2021 and analyzed using IBM SPSS 27.0.
Results

Compared with the youngest participants (<21 years) knowledge of fermentation was higher in older individuals (p<0.001). More than a half of the participants recognized the role of lactic acid bacteria and yeasts in fermentation process, however, only 18.3% of participants were aware of the role of the molds. Only 25.9% of the participants have become acquainted with fermented foods at home and 62.2% of them were aware of health benefits of fermented foods, but mostly on gastrointestinal health and the immune system.
Conclusions

As people today live predominantly in urban areas and incline towards westernized foods, they often lack the knowledge of fermentation and awareness regarding the nutritional value of fermented foods and their preparation. Steps should be taken to educate younger generations regarding the health benefits of fermented foods especially considering that most of them expressed their interest in learning more about the process.