bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification of fibronectin 1 as a candidate genetic modifier in a Col4a1 mutant mouse model of Gould syndrome
Authors: Mao Mao , Popli Tanav , Jeanne Marion , Hoff Kendall , Sen Saunak , Gould Douglas B. 
Published: March 2021

ABSTRACT
Collagen type IV alpha 1 and alpha 2 (COL4A1 and COL4A2) are major components of almost all basement membranes. COL4A1 and COL4A2 mutations cause a multisystem disorder that can affect any organ but typically involves the cerebral vasculature, eyes, kidneys and skeletal muscles. In recent years, patient advocacy and family support groups have united under the name of Gould syndrome. The manifestations of Gould syndrome are highly variable, and animal studies suggest that allelic heterogeneity and genetic context contribute to the clinical variability. We previously characterized a mouse model of Gould syndrome caused by a Col4a1 mutation in which the severities of ocular anterior segment dysgenesis (ASD), myopathy and intracerebral hemorrhage (ICH) were dependent on genetic background. Here, we performed a genetic modifier screen to provide insight into the mechanisms contributing to Gould syndrome pathogenesis and identified a single locus [modifier of Gould syndrome 1 (MoGS1)] on Chromosome 1 that suppressed ASD. A separate screen showed that the same locus ameliorated myopathy. Interestingly, MoGS1 had no effect on ICH, suggesting that this phenotype could be mechanistically distinct. We refined the MoGS1 locus to a 4.3 Mb interval containing 18 protein-coding genes, including Fn1, which encodes the extracellular matrix component fibronectin 1. Molecular analysis showed that the MoGS1 locus increased Fn1 expression, raising the possibility that suppression is achieved through a compensatory extracellular mechanism. Furthermore, we found evidence of increased integrin-linked kinase levels and focal adhesion kinase phosphorylation in Col4a1 mutant mice that is partially restored by the MoGS1 locus, implicating the involvement of integrin signaling. Taken together, our results suggest that tissue-specific mechanistic heterogeneity contributes to the variable expressivity of Gould syndrome and that perturbations in integrin signaling may play a role in ocular and muscular manifestations.
Picornavirus 3C – a protease ensuring virus replication and subverting host responses
Authors: Yi Jiamin , Peng Jiangling , Yang Wenping , Zhu Guoqiang , Ren Jingjing , Li Dan , Zheng Haixue 
Published: March 2021

ABSTRACT
The protease 3C is encoded by all known picornaviruses, and the structural features related to its protease and RNA-binding activities are conserved; these contribute to the cleavage of viral polyproteins and the assembly of the viral RNA replication complex during virus replication. Furthermore, 3C performs functions in the host cell through its interaction with host proteins. For instance, 3C has been shown to selectively ‘hijack’ host factors involved in gene expression, promoting picornavirus replication, and to inactivate key factors in innate immunity signaling pathways, inhibiting the production of interferon and inflammatory cytokines. Importantly, 3C maintains virus infection by subtly subverting host cell death and modifying critical molecules in host organelles. This Review focuses on the molecular mechanisms through which 3C mediates physiological processes involved in virus–host interaction, thus highlighting the picornavirus-mediated pathogenesis caused by 3C.
Education and training to support physiotherapists working in dementia care: a scoping review protocol
Authors: O'Sullivan Trish , Foley Tony , McVeigh Joseph G. , Timmons Suzanne 
Published: March 2021

Background: The care of people with dementia is of global concern. Physiotherapeutic intervention can be of benefit to patients with dementia. Physiotherapists can play a role in assessment, falls prevention, pain management and gait re-education. Dementia care forms a significant part of the workload of a physiotherapist. However, there is a paucity of evidence on what constitutes effective education and training for physiotherapists working in dementia care. Objective: This scoping review aims to explore and map the evidence on education and training for physiotherapists working in dementia care. Inclusion criteria: Studies that explore dementia training and/or education for physiotherapists or for multidisciplinary teams, in which physiotherapists have been included. Studies that explore student physiotherapy training will also be considered. Systematic reviews, qualitative, quantitative, mixed methods studies, case studies and observational studies will be included. Methods: This scoping review will follow the Joanna Briggs Institute (JBI) methodology for scoping reviews. Databases to be searched as part of this review include: Medline, Cochrane Database of Systematic Reviews, SocINDEX, CINAHL and, PsycINFO, with no limitation on publication date. Google Scholar and Open-Grey will be searched for grey literature, limited to the first 100 searches. Titles and abstracts will be screened for inclusion and identified full texts reviewed independently by two reviewers. Data will be extracted using a draft data extraction tool based on the JBI data extraction tool. A chronological narrative synthesis of the data will outline how the results relate to the aims and objective of this scoping review.
Growth pattern of three species of Mullets (Pisces: Mugilidae) in Sombreior River, Rivers State, Niger Delta, Nigeria
Authors: Dienye Henry Eyina , Olopade Olaniyi Alaba 
Published: March 2021

Length-weight relationships (LWRs) and condition factor are important biological information to assess the growth pattern and wellbeing of fish species influenced by numerous abiotic and biotic factors. To date, no data on the LWRs and condition of mullets in Sombreior River, Niger Delta. Hence, this research was conducted to determine abundance, length–weight relationship and Fulton’s condition factor (K) of three species of fish belonging to family Mugilidae in Sombreior River, Nigeria. There were a total of 248 samples from five sampling stations for a period of months. Fish species were of various sizes ranging from 24.66±0.40 to 26.35±0.26cm in length and 141.41±6.30 to 161.56±5.87g in weight. For the three species, slope (b) values ranged from 2.18 to 2.68 indicating a negative allometric growth of all fish species. The mean condition factor ranged from 0.89±0.03 forsix Mugil cephalus to 0.93±0.03 for Parachelon grandisquamis while the coefficients of determination (R2) of the LWR regressions ranged between 0.64 (Neochelon falcipinnis) and 0.79 (Parachelon grandisquamis). It was concluded that the mullets in the Sombreior River had a negative allometric growth pattern and the condition is showing the species were in good state of well-being since mean K values are greater than 1.
Effect of vitamin E on the quality of egg bonylip barb fish Osteochilus vittatus (Valenciennes, 1842)
Authors: Tarigan Nurbety , Affandi Ridwan , Meiyasa Firat 
Published: March 2021

Bonylip barb Osteochilus vittatus is a local fish species of significant economic value with vast potential in commercial freshwater fish culture. Traditional cultivation methods are currently employed depending on the seeds, although a low hatchery rate is assumed. The purpose of this study, therefore, was to determine the effect of dietary vitamin E on the quality of bonylip barb eggs, with the applied dosage of 0, 125, 250, and 375 mg/kg. Subsequent parameters measured include fecundity, drowned egg percentage, the chemical composition of eggs, and total energy retention. The results showed that several vitamin E doses significantly (P˂0.05) influenced these aforementioned constraints. Furthermore, the application of vitamin E at 375 mg/kg recorded the best dosage to improve egg quality. In addition, the fecundity was estimated at 23.484 grains, and sink egg percentage reached 92.66%, while protein and fat content were 21.43 mg/kg and 27.88%higher compared to the controlled sample. Therefore, It is concluded that the administration of vitamin E in the feed tends to increase egg quality of bonylip barb fish.
Effect of asam kandis (Garcinia cowa Roxb) extract in drinking water on egg quality of Japanese quail (Coturnix coturnix japonica)
Authors: Darmawan Arif , Hermana Widya , Dirgantara Muhamad Prastyo 
Published: March 2021

Asam kandis (Garcinia cowa Roxb) fruit has been reported to contain phytochemicals such as xanthon, hydroxy cytric acid and flavonoids that have various activities such as antimicrobial and antioxidant. This study was aimed to investigate the effect of asam kandis extract addition in drinking water on the quality of quail eggs. Ninety nine laying quails of 59 days of age were divided into 3 treatments and 3 replications with 11 laying quails in each replication. Completely Randomized Design was used and the treatments were: T1= control (drinking water without asam kandis extract), T2= drinking water with asam kandis extract at pH 4, T3= drinking water with asam kandis extract at pH 3. The parameters observed were egg weight, egg shape index, yolk color, yolk percentage, albumen percentage, eggshell percentage, shell thickness, and haugh unit score. The data obtained were analyzed by Analysis of Variance (ANOVA) and significant differences among treatments means were tested by Duncans multiple test. The the addition of asam kandis extract did not affect (P0.05) on egg weight, egg shape index, yolk color, yolk percentage, albumen percentage, eggshell percentage, and haugh unit score. The addition of asam kandis extract in drinking water at pH 3 significantly (P0.05) increased eggshell thickness compare to that of other treatments. The conclusion was the addition of asam kandis extract in drinking water at pH 3 increased eggshell thickness without decreasing egg quality.
Komposisi biota dasar hasil tangkapan alat garok pada perairan pesisir Kronjo, Tangerang
Authors: Yonvitner Yonvitner , Dahuri Rokhmin , Andi Isdradjad Setyobudi , Boer Mennofatria 
Published: March 2021

Garok is a fishing gear that operates at the bottom or surface of the substrate that is caught of various benthic species. The garok gear that operates in sediment, even in the long term can cause changes in the composition of the biota. Because exploitative fishing proses, can cause damage, vulnerability and at the long-term impact to the sustainability of the population. The research was carried out in Kronjo Bay, Tangerang from March to May 2011. The samples were collected from the operation, then determined the species, quantity, and weight of each species. Descriptive statistical analysis and ANOVA were used to determine the significance of the composition between stations and observation times. The caught consist of Placuna placenta, Anadara, Murex, Tellina, and crustaceans which belong to the mollusk and crustacean groups. Statistical analysis did not show a significant difference between the research stations, but it was significantly based on the observation time with Fhit 3,1 and Ftab 1,7. It turned out that the abundance of basic biota was found to be high in April then decreased in May. Likewise, the abundance did not show a significant difference in the location and time of observation. The catch composition on the Kronjo coast is dominated by the gastropod group with an average composition of above 50% per operation.Keywords:GarokCompositionDemersalKronjoCoastalABSTRAKGarok adalah alat tangkap yang dioperasikan di dasar perairan yang menangkap berbagai jenis biota dasar. Alat garok yang dioperasikan dapat menangkap beragam jenis biota dasar dan dalam jangka lama dapat menyebabkan terjadinya perubahan komposisi biota. Alat tangkap yang bersifat eksploitatif dapat menyebabkan kerusakan, kerentanan sehingga menganggu keberlanjutan populasi secara jangka panjang. Penelitian dari praktek penggunaan alat garok ini dilakukan di Teluk Kronjo Tangerang mulai Maret-Mei tahun 2011. Sampel dikumpulkan secara eskploratif dari operasi alat garok, kemudian tentukan jenis, jumlah dan bobotnya dari setiap jenis. Analisis statistik deskriptif dan ANOVA digunakan untuk mengetahui perbedaan komposisi antar stasiun dan antara waktu pengamatan. Hasil tangkapan terdiri dari jenis Placuna placenta, Anadara, Murex, Tellina, dan krustasea yang termasuk kelompok moluska dan krustasea. Analisis statistik tidak menunjukan perbedaan yang nyata antara stasiun penelitian, namun berbeda nyata berdasarkan waktu pengamatan dengan Fhit 3,1 dan Ftab 1,7. Kelimpahan biota dasar ditemukan tinggi pada bulan April kemudian menurun pada bulan Mei. Begitu juga kelimpahan tidak menunjukan perbedaan yang nyata pada lokasi dan waktu pengamatan. Komposisi tangkapan di pesisir Kronjo didominasi oleh kelompok gastropoda dengan komposisi rata-rata diatas 50% setiap kali operasi.Kata kunci:GarokKomposisiDemersalKronjoPesisir
Analisis dinamika populasi ikan selar kuning (Selaroides leptolepis) dalam upaya pengelolaan sumberdaya ikan pelagis kecil di perairan Bintan, Provinsi Kepulauan Riau
Authors: Septiyawati Septiyawati , Fauzi Muhammad , Efizon Deni 
Published: March 2021

This raw fish is used as fisheries products such as salted fish, grilled fish, fishmeal and surimi. This study aims to analyze mortality and recruitment of yellowstipe scad (Selaroides leptolepis) and the information about management of small pelagic fisheries resources in Bintan Waters. This research was carried out in February-June 2019 at the Barek Motor Fish Landing Area (TPI) with fishing areas in Bintan waters. The number of fish studied was 2.550 with a length of 102-215 mm and a weight of 15-102 gr. The highest recruitment peak occurred in Mei of 18.11% and June of 19.48%. The highest and lowest condition factors are 0.83 and 1.09. The total mortality rate (Z) of yellow strait fish is 4.61 per year with a natural mortality rate (M) of 1.66 per year and the capture mortality rate of 2.95 per year so that the exploitation rate is 0.63. The death of yellowstipe scade in the Bintan Sea most of the caused by fishing activities (F) of 2.95 per year.Keywords:Yellowstipe scad Bintan WatersRecrutmentCondition factorsMortalityABSTRAKIkan selar kuning merupakan salah satu jenis ikan ekonomis penting. Ikan ini banyak dimanfaatkan sebagai bahan baku produk olahan perikanan seperti ikan asin, ikan bakar, pindang, tepung ikan dan surimi. Penelitian ini bertujuan untuk menganalisis, mortalitas dan rekrutmen ikan selar kuning (Selaroides leptolepis) dan informasi tentang pengelolaan sumberdaya ikan pelagis kecil di Perairan Bintan. Penelitian ini telah dilaksanakan bulan Februari-Juni 2019 di Tempat Pendaratan Ikan (TPI) Barek Motor dengan wilayah penangkapan di perairan Bintan. Jumlah ikan yang diteliti 2.550 ekor dengan panjang 102-215 mm dan berat 15-102 gr. Puncak rekrutmen tertinggi terjadi bulan Mei sebesar 18,11% dan Juni sebesar 19,48%,. Nilai tertinggi dan terendah faktor kondisi yaitu 0,82 dan 1,09. Laju mortalitas total (Z) ikan selar kuning 4,61 per tahun dengan laju mortalitas alami (M) 1,66 per tahun dan laju mortalitas penangkapan 2,95 per tahun sehingga diperoleh laju eksploitasi 0,63. Kematian ikan selar kuning di laut Bintan sebagian besar diakibatkan oleh aktivitas penangkapan (F) sebesar 2,95 per tahun.Kata kunci:Ikan selar kuning Perairan Bintan Rekrutmen Faktor kondisi Mortalitas
High-power sub-15  fs nonlinear pulse compression at 515  nm of an ultrafast Yb-doped fiber amplifier
Authors: Descamps Dominique , Guichard Florent , Petit Stéphane , Beauvarlet Sandra , Comby Antoine , Lavenu Loïc , Zaouter Yoann 
Published: March 2021

We present an efficient and robust scheme to produce energetic sub-15 fs pulses centered at 515 nm with a peak power exceeding 3 GW. Combining efficient second-harmonic generation of a 135 fs, 50 W Yb-doped fiber amplifier with a low-loss capillary-based visible pulse compression stage, we reach an overall efficiency higher than

>

20

%

. The system is also designed to take advantage of the repetition rate flexibility of the fiber amplifier, leading sub-15 fs pulse generation from 166 to 500 kHz with an average power exceeding the 10 watt level. The combined reduction of the laser wavelength and pulse duration is expected to highly improve the yield of high-order harmonic generation to provide high photon flux of ultrashort extreme ultraviolet radiation.
Post-intensive care syndrome as a predictor of mortality in patients with critical illness: A cohort study
Authors: Yanagi Naoya , Kamiya Kentaro , Hamazaki Nobuaki , Matsuzawa Ryota , Nozaki Kohei , Ichikawa Takafumi , Valley Thomas S. , Nakamura Takeshi , Yamashita Masashi , Maekawa Emi , Koike Tomotaka , Yamaoka-Tojo Minako , Arai Masayasu , Matsunaga Atsuhiko , Ako Junya 
Published: March 2021

Introduction
The post-intensive care syndrome (PICS) encompasses multiple, diverse conditions, such as physical disability, cognitive impairment, and depression. We sought to evaluate whether conditions within PICS have similar associations with mortality among survivors of critical illness.

Materials and methods
In this retrospective cohort study, we identified 248 critically ill patients with intensive care unit stay ≥72 hours, who underwent PICS evaluation. Patients with disability in activities of daily living, cognitive impairment, or depression before hospitalization were excluded. We defined PICS using established measures of physical disability (usual gait speed), cognitive impairment (Mini-Cog test), and depression (Patient Health Questionnaire-2) at hospital discharge. The endpoint was all-cause mortality.

Results
Patients had a median age of 69 years and Acute Physiology and Chronic Health Evaluation (APACHE) II score of 16. One hundred thirty-two patients were classified as having PICS, and 19 patients died. 81/248 (34%) patients had physical disability, 42/248 (19%) had cognitive impairment, and 44/248 (23%) had depression. After adjusting for covariates on multivariable Cox regression analyses, PICS was significantly associated with all-cause mortality (hazard ratio [HR] 3.78, 95% confidence interval [CI] 1.02 – 13.95; P = 0.046). However, the association between PICS and all-cause mortality was related to physical disability and cognitive impairment (P = 0.001 and P = 0.027, respectively), while depression was not (P = 0.623).

Conclusion
While PICS as a syndrome has been useful in gaining attention to the sequelae of critical illness, its relationship with long-term mortality is driven largely by physical disability and cognitive impairment and not depression.
Impact of ceftriaxone and temocillin on fecal abundance of extended-spectrum β-lactamase producing Escherichia coli in a mouse model
Authors: Chenouard Rachel , Mahieu Rafael , Luque Paz David , Marion Estelle , Eveillard Matthieu , Dubée Vincent 
Published: March 2021

Background
Gut colonization by ESBL-producing Enterobacteriaceae (ESBL-PE) is widespread and is promoted by antibiotic exposure. Higher fecal abundance of ESBL-PE promotes the dissemination of the bacteria in the environment and is associated with increased risk of infection. Ceftriaxone and temocillin are commonly used antibiotics with a different activity on gut flora. Their impact on fecal abundance of ESBL-producing Enterobacteriaceae has not been studied. The objective of this study was to compare the propensity of ceftriaxone and temocillin to modify the abundance of ESBL-producing Escherichia coli in feces of colonized mice.

Methods
Mice received broad-spectrum antibiotics in order to disrupt their normal gut flora. A CTX-M-type ESBL-producing E. coli clinical isolate was then administered orally, leading to durable colonization. Thirty days later, mice received either temocillin or ceftriaxone with drinking water at a concentration simulating human intestinal exposure. Third-generation-cephalosporin resistant (3GCR) E. coli were enumerated in feces on selective medium before, 2 days and 10 days after the end of antibiotic exposure. The experiment was performed with two E. coli isolates with different temocillin minimum inhibitory concentrations.

Results
Exposure to ceftriaxone induced an increase in the fecal abundance of 3GCR E. coli. In contrast, temocillin had no effect or transiently decreased the number of 3GCR E. coli. Results obtained with the two strains were similar.

Conclusion
Contrary to ceftriaxone, temocillin does not promote expansion of ESBL-producing E. coli in feces of colonized mice. Thus temocillin may be a therapeutic of choice when a temocillin-susceptible strain infection is suspected or proven to prevent the expansion of ESBL-PE in a previously colonized patient.
Challenging drug-resistant TB treatment journey for children, adolescents and their care-givers: A qualitative study
Authors: Das Mrinalini , Mathur Taanya , Ravi Shilpa , Meneguim Augusto C. , Iyer Aparna , Mansoor Homa , Kalon Stobdan , Hossain Farah Naz , Acharya Shrikala , Ferlazzo Gabriella , Isaakidis Petros , Thakur Harshad P. 
Published: March 2021

Background
Childhood multidrug-resistant TB (MDR-TB) still affects around 25000 children every year across the globe. Though the treatment success rates for drug-resistant TB (DR-TB) in children are better than adults, children and adolescents face unique hurdles during DR-TB (MDR-TB, Pre-XDR TB and XDR-TB) treatment. This study aimed to understand the patients, guardians and healthcare providers’ perspectives about DR-TB treatment journey of patients and caregivers.

Methods
This is a qualitative study involving in depth-interviews of purposively selected adolescents (n = 6), patients guardians (for children and adolescents, n = 5) and health care providers (n = 8) of Médecins Sans Frontières (MSF) clinic, Mumbai, India. In-depth face to face interviews were conducted in English or Hindi language using interview guides during September-November 2019. The interviews were audio-recorded after consent. Thematic network analysis was used to summarize textual data. ATLAS.ti (version 7) was used for analysis.

Result
The age of adolescent patients ranged from 15–19 years and four were female. Five guardians (of three child and two adolescent patients) and eight healthcare providers (including clinicians- 2, DOT providers-2, counselors-2 and programme managers-2) were interviewed. The overarching theme of the analysis was: Challenging DR-TB treatment journey which consisted of four sub-themes: 1) physical-trauma, 2) emotional-trauma, 3) unavailable social-support and 4) non-adapted healthcare services. Difficulties in compounding of drugs were noted for children while adolescents shared experiences around disruption in social life due to disease and treatment. Most of the patients and caregivers experienced treatment fatigue and burnout during the DR-TB treatment. Participants during interviews gave recommendations to improve care.

Discussion
The TB programmes must consider the patient and family as one unit when designing the package of care for paediatric DR-TB. Child and adolescent friendly services (paediatric-formulations, age-specific counselling tools and regular interaction with patients and caregivers) will help minimizing burnout in patients and caregivers.