bluuebunny/crossref_metadata_2025_split huggingface dataset:
Circ_0072088 promotes progression of hepatocellular carcinoma by activating JAK2/STAT3 signaling pathway via miR‐375
Authors: Li Liheng , Xiao Chengjiang , He Ke , Xiang Guoan 
Published: June 2021

AbstractCircular RNAs feature prominently in cancer development. Nonetheless, the role of circ_0072088 in hepatocellular carcinoma (HCC) remains unclear. GEO databases (GSE97332, GSE108724, GSE36915, and GSE33006) were used to screen out the differentially expressed circRNAs, miRNAs, and mRNA in HCC. The expressions of circ_0072088, miR‐375, and Janus Kinase 2 (JAK2) mRNA in HCC tissue and cell lines were determined with quantitative real‐time polymerase chain reaction. RNase R treatment assay was used to measure the stability of circ_0072088, and subcellular fraction assay was used to detect the localization of circ_0072088. Cell counting kit‐8 assay, flow cytometry, and Transwell assay were used to measure proliferation, apoptosis, migration, and invasion of HCC cells. RNA immunoprecipitation and dual‐luciferase reporter gene assay were employed for investigating the binding sequence between circ_0072088 and miR‐375, as well as miR‐375 and JAK2 3′UTR. Western blot assay was used to detect the expression of JAK2 and p‐STAT3 after circ_0072088 and miR‐375 were selectively regulated. Circ_0072088 and JAK2 mRNA expressions were highly expressed in HCC tissues and cell lines while miR‐375 expression was remarkably downregulated. Circ_0072088 was resistant to RNase R treatment and mainly located in the cytoplasm of HCC cells. The transfection of circ_0072088 overexpression plasmid or miR‐375 inhibitors promoted the proliferation, migration, and invasion, and inhibited the apoptosis of HCC cells, whereas transfection of circ_0072088 siRNA or miR‐375 mimics exerted opposite effects. Besides, miR‐375 was confirmed as a target of circ_0072088 and miR‐375 could further downregulate the expression of JAK2. MiR‐375 mimics could reverse the upregulation of JAK2 and p‐STAT3 protein induced by circ_0072088 overexpression. Circ_0072088 can enhance the proliferation, migration, and invasion, and impede apoptosis of HCC cells. Mechanistically, circ_0072088 activates JAK2/STAT3 signaling pathway by serving as a molecular sponge of miR‐375.
High‐flux strategy for electrospun nanofibers in membrane distillation to treat aquaculture wastewater: a review
Authors: Zhou Lei , Tan Soon Huat , Ahmad Abdul Latif , Low Siew Chun 
Published: June 2021

AbstractAquaculture wastewater contains a large amount of nitrogen and phosphorus compounds, which has a serious impact on the environment and is also harmful to aquatic life. Such wastewater also contains high salinity, especially for mariculture activities. This review summarizes the recent progress and potential of electrospun nanofiber membranes (ENMs) in membrane distillation (MD) to treat high‐salinity aquaculture wastewater. Superhydrophobic membranes, which display a prominent wetting resistance, have been widely used to improve the efficiency of the MD process. The intuitive hypothesis is that a membrane with a low surface energy and a low sliding angle has a higher slip effect, thereby improving the wetting resistance of the membrane. Based on this perspective, this review focuses on the wetting tendency of parahydrophobic ENMs with high water adhesion and membrane design for MD wettability. Various strategies are reviewed, including the techniques to induce surface roughness during membrane fabrication and the post‐membrane modification to improve the wetting resistance of MD for long‐term operation. This review provides an in‐depth analysis and a fundamental interpretation of novel perspectives of ENMs for more advanced MD applications. © 2021 Society of Chemical Industry (SCI).
An autochthonous aerobic bacterial community and its cultivable isolates capable of degrading fluoxetine
Authors: Luz Palma Tânia , Shylova Anastasiia , Dias Carlier Jorge , Costa Maria Clara 
Published: June 2021

AbstractBACKGROUNDFluoxetine is an antidepressant and recalcitrant fluorine pharmaceutical that is poorly biodegraded, so it enters the hydric resources and causes hazardous effects to aquatic environments. According to these fluoxetine features, the main aim of the present research was to find resistant bacteria in environmental samples with a high degradation efficiency.RESULTSThe results obtained from raw municipal wastewater spiked with fluoxetine and inoculated with aerobic sludge from a Portuguese wastewater treatment plant under highly aerobic conditions showed that more than half and ≈89% of the drug was degraded after 48 and 144 h, respectively. During the assay, the initial population (mainly composed of Arcobacter, Bacteroides, and Macellibacteroides) shifted with an increase of members of the Acinetobacter, Rheinheimera, Shewanella, Pseudomonas, Methylobacillus, Piscinobacter genera and Aeromonadales order and the Pseudomonadaceae family, all of which were likely responsible for fluoxetine biodegradation. From the same sludge, six bacterial isolates were selected and identified as follows: Pseudomonas putida, Enterobacter ludwigii, Pseudomonas nitritireducens, Alcaligenes faecalis, Pseudomonas aeruginosa, and Pseudomonas nitroreducens; all of them grew with fluoxetine as sole carbon source. Pseudomonas nitroreducens showed the highest removal of 55 ± 1% at 20 mg L–1 fluoxetine after 24 h.CONCLUSIONAn autochthonous aerobic bacterial community and its cultivable isolates showed the capacity to biodegrade fluoxetine. Biodegradation, rather than adsorption, appears to play the main role in the fluoxetine removal in aerobic conditions using bacteria simply obtained from an environmental sample. As far as is known, those bacteria are reported for the first time as fluoxetine biodegraders; thus, these bacteria are a promising option to integrate into new bioremediation processes aiming at the removal of fluoxetine. © 2021 Society of Chemical Industry (SCI).
ABCMETAapp: R shiny application for simulation‐based estimation of mean and standard deviation for meta‐analysis via approximate Bayesian computation
Authors: Kwon Deukwoo , Reddy Roopesh Reddy Sadashiva , Reis Isildinha M. 
Published: June 2021

AbstractIn meta‐analysis based on continuous outcome, estimated means and corresponding standard deviations from the selected studies are key inputs to obtain a pooled estimate of the mean and its confidence interval. We often encounter the situation that these quantities are not directly reported in the literatures. Instead, other summary statistics are reported such as median, minimum, maximum, quartiles, and study sample size. Based on available summary statistics, we need to estimate estimates of mean and standard deviation for meta‐analysis. We developed an R Shiny code based on approximate Bayesian computation (ABC), ABCMETA, to deal with this situation. In this article, we present an interactive and user‐friendly R Shiny application for implementing the proposed method (named ABCMETAapp). In ABCMETAapp, users can choose an underlying outcome distribution other than the normal distribution when the distribution of the outcome variable is skewed or heavy tailed. We show how to run ABCMETAapp with examples. ABCMETAapp provides an R Shiny implementation. This method is more flexible than the existing analytical methods since estimation can be based on five different distributions (Normal, Lognormal, Exponential, Weibull, and Beta) for the outcome variable.
Chemometric evaluation of functional components and anti‐quorum sensing activity of mulberry leaves from Indian cultivars: a potential contribution to the food industry
Authors: Shreelakshmi Saligrama Viswanath , Nazareth Maria Sheeba , Matam Pradeep , Dorairaj Darshan , Shetty Nandini P 
Published: June 2021

AbstractBACKGROUNDPotential use of many native, easily available vegetal materials for human consumption and value addition is not well recognized. Mulberry, being a traditional industrial crop rich in nutrients and nutraceuticals can be of great importance for the food industry. However, mulberry leaves are mainly being utilized in sericulture and are not exploited for their functional components. Thus, the selection of promising mulberry cultivars, rich in bioactive compounds, like resveratrol and 1‐deoxynojirimycin, increase their potential use in functional foods.RESULTSChlorogenic acid, myricetin and kaempferol were the major polyphenols present in the nine selected cultivars, in the range 0.001–0.086, 0.003–0.079 and 0.003–0.163 g kg−1fresh weight (FW), respectively. Protocatechuic acid, epicatechin and rutin were predominantly present in cultivars V‐1, G‐2 and ML (0.103, 0.080 and 0.121 g kg−1FW, respectively). Similarly, resveratrol and 1‐deoxynojirimycin were highest in cultivars ML and K‐2 (0.078 and 0.079 g kg−1FW, respectively). Leaf extracts of cultivars G‐2 and ML were able to effectively inhibit the violacein production with 64.08% and 70.04%, respectively at the concentration of 6 mg mL−1presumably due to a higher content of polyphenols. Chemometric evaluation of chromatographic data showed the intraspecific variability and secondary metabolite co‐existence in different cultivars.CONCLUSIONSConsidering phytoconstituents, cultivars G‐2, ML, K‐2 and V‐1 could contribute efficiently to the rational utilization of mulberry in agro‐food industries. Furthermore, cultivars G‐2 and ML leaves can be a new source of quorum sensing inhibitory agents. © 2021 Society of Chemical Industry.
Effects of yeast hydrolysate on growth performance, serum parameters, carcass traits, meat quality and antioxidant status of broiler chickens
Authors: Wang Ting , Cheng Kang , Yu CaiYun , Tong YiChun , Yang ZaiBin , Wang Tian 
Published: June 2021

AbstractBACKGROUNDYeast hydrolysate (YH) has multiple salutary biological activities. Nevertheless, the application of YH in broiler production is limited. This study was conducted to evaluate the protective effects of YH derived from Saccharomyces cerevisiae by exploring growth performance, serum parameters, organs relative weight, carcass traits, meat quality and antioxidant status of broilers.RESULTSSupplementing YH linearly and quadratically improved (P < 0.05) body weight gain and gain‐to‐feed ratio compared to that in the control group. Triglycerides, low‐density lipoprotein cholesterol and total cholesterol in serum, the decline in pH and cooking loss of breast muscle, and malonaldehyde concentration in serum and liver were decreased linearly and/or quadratically by YH (P < 0.05), whereas high‐density lipoprotein cholesterol in serum, superoxide dismutase (SOD) and glutathione peroxidase (GSH‐Px) activities in serum, GSH‐Px activity in liver, glutathione content in serum and liver, eviscerated yield rate and chest muscle yield, and the relative weight of spleen and liver were linearly and/or quadratically increased (P < 0.05). Moreover, YH enhanced the mRNA levels of nuclear factor erythroid 2‐related factor 2, heme oxygennase‐1 (HO‐1), GSH‐Px1 and SOD1 (linear and/or quadratic, P < 0.05).CONCLUSIONDietary YH beneficially affected growth performance, serum parameters, organ relative weight, carcass traits, meat quality and antioxidant status in broilers, indicating its potential application as a promising feed additive in broiler production. © 2021 Society of Chemical Industry.
Effects of 25‐hydroxyvitamin D3 on growth performance, serum parameters, fecal microbiota, and metabolites in weaned piglets fed diets with low calcium and phosphorus
Authors: Zhang Lianhua , Yang Ming , Piao Xiangshu 
Published: June 2021

AbstractBACKGROUNDWith the development of intensive farming, long‐term exposure of pigs to poor light conditions is not conducive to the production of vitamin D3, and vitamin D3 deficiency could affect absorption and metabolism of calcium (Ca) and phosphorus (P). 25‐Hydroxyvitamin D3 (25OHD3) has higher bioactivity than regular vitamin D3. This study investigated the effects of 25OHD3 on performance, serum parameters, fecal microbiota, and metabolites in weaned piglets fed with low Ca‐P diet.RESULTSIt was found that a low Ca‐P diet supplemented with 50 μg/kg 25OHD3 (NC + 25‐D) improved (P < 0.05) average daily gain (ADG) in phase 2 and in the overall period of the experiment, and increased (P < 0.05) the immunoglobulin G (IgG), immunoglobulin A (IgA), catalase (CAT), bone‐specific alkaline phosphatase (BALP), and osteocalcin (OC) serum content on day 28 compared with a low Ca‐P diet (NC), but no differences were observed between a normal Ca‐P diet (PC) and the NC + 25‐D diet. Compared with NC, the abundance of Firmicutes was higher (P < 0.05) in PC and NC + 25‐D. NC + 25‐D decreased (P < 0.05) the abundance of Streptococcaceae compared with PC and NC, and increased (P < 0.05) the abundance of Lachnospiraceae compared with NC. Serum 25OHD3 was negatively correlated with the abundance of fecal Streptococcaceae (P < 0.05), and positively correlated with the abundance of fecal Lachnospiraceae (P < 0.05).CONCLUSIONSupplementation of 25OHD3 in a low Ca‐P diet improved serum immunity, bone biochemical parameters, and fecal microbiota such as decreased Streptococcaceae abundance and increased Lachnospiraceae abundance, which could subsequently promote growth of piglets. The effects were similar to that of a normal Ca‐P diet. © 2021 Society of Chemical Industry.
Research advancement and commercialization of microalgae edible oil: a review
Authors: Xue Zhaohui , Li Shihao , Yu Wancong , Gao Xin , Zheng Xu , Yu Yue , Kou Xiaohong 
Published: June 2021

AbstractThe global food crisis has led to a great deal of attention being given to microalgal oil as a sustainable natural food source. This article provides an overview of the progress and future directions in promoting the commercialization of microalgal edible oils, including microalgal triglyceride accumulation, suitable edible oil culture strategies for high nutritional value, metabolic engineering, production, and downstream technologies. The integration of the production process, biosafety, and the economic sustainability of microalgal oil production are analyzed for their critical roles in the commercialization of microalgal edible oil to provide a theoretical and scientific basis for the comprehensive development and utilization of microalgal edible oil. © 2021 Society of Chemical Industry.
Determination of heterocyclic amines in braised sauce beef and the effects of different cooking conditions on the formation of heterocyclic amines
Authors: Zhou Ya Jun , Zhang Yu Xia , Dong Xue Wen 
Published: June 2021

AbstractBACKGROUNDCooked meat is a good source of high‐quality protein. However, during long‐term cooking of meat, radical‐induced lipid and protein oxidation may lead to the formation of poisonous compounds, such as heterocyclic amines (HAs). This work investigated a testing method for HAs and it describes that cooking temperature, cooking time and repeated cooking times have influences on the overall quality of braised sauce beef and the effect of HAs formation.RESULTSThe improved method has a good separation effect on nine kinds of HAs. The average recovery of HAs at two spiked levels is between 51.70% and 88.25% (n = 6). The detection limit is 0.025–0.060 ng g–1, and the limit of quantitation is 0.070–0.160 ng g–1. Only harman and norharman were detected in samples. Cooking time and cooking temperature will affect the quality and HA content of samples. When the braised sauce beef soup was cooked 20 times, the HA content was the highest – as high as 21.43 ng g–1. The more times the beef was cooked repeatedly, the higher was the HA content. Under different cooking conditions, glucose has a significant effect on the formation of β‐carboline.CONCLUSIONWe have established a detection method for HAs with good repeatability and accuracy. HAs were more easily formed in braised sauce beef by high temperature, long heating and repeated brine cooking. © 2021 Society of Chemical Industry.
Shift in archaeal community along a soil profile in coastal wheat‐maize rotation fields of different reclamation ages
Authors: Wan Shaoyu , Liao Xiaolin , Zhou Tong Tong , Wu Yan , Hu Anyong , Yan Dezhi , Zhang Jiao , Long Xi‐En 
Published: June 2021

AbstractCoastal mudflats reclamation activities show a strong influence on soil properties and microbial community activity, which drive soil carbon and nitrogen cycling. Studies have found there is a shift of bacterial community with reclamation time. However, effects of reclamation time on the soil archaeal community are still largely unknown. Here, we determined soil properties and archaeal community in the soil profiles (0–15, 15–30, 30–45 cm) of different reclaimed sites with different reclamation ages (R12: 12‐year; R38: 38‐year; R68: 68‐year; R100: 100‐year) in Rudong County, Jiangsu, China. Soil archaeal communities were identified via Illumina high‐throughput sequencing. Soil archaeal communities were dominated by two phyla, the Crenarchaeota (82.1–93.3%) and Thermoplasmatota (4.2–17.9%), which are associated with nitrogen cycle (Nitrosopumilaceae, Nitrososphaeraceae, and Nitrosotaleaceae) and organic carbon metabolism (Marine_Group_II), respectively. Ammonia oxidizing archaea showed a divergent response to the reclamation, i.e. Nitrosotaleaceae increased with reclamation time whereas Nitrosopumilaceae showed a decreasing trend. Soil total carbon, total nitrogen, pH, EC, C:N ratio, NO3−‐N content, soil moisture, depth, and reclamation age determined archaeal community distribution in our reclaimed sites. Our findings suggest that both reclamation time and soil depth significantly shaped the archaeal community composition, and then in turn modulated soil carbon and nitrogen cycling in the reclaimed mudflats.
Recent pharmacological advances in the repurposing of artemisinin drugs
Authors: Meng Yuqing , Ma Nan , Lyu Haining , Wong Yin Kwan , Zhang Xing , Zhu Yongping , Gao Peng , Sun Peng , Song Yali , Lin Lizhu , Wang Jigang 
Published: June 2021

AbstractArtemisinins are a family of sesquiterpene lactones originally derived from the sweet wormwood (Artemisia annua). Beyond their well‐characterized role as frontline antimalarial drugs, artemisinins have also received increased attention for other potential pharmaceutical effects, which include antiviral, antiparsitic, antifungal, anti‐inflammatory, and anticancer activities. With concerted efforts in further preclinical and clinical studies, artemisinin‐based drugs have the potential to be viable treatments for a great variety of human diseases. Here, we provide a comprehensive update on recent reports of pharmacological actions and applications of artemisinins outside of their better‐known antimalarial role and highlight their potential therapeutic viability for various diseases.
Proteomics support the threespine stickleback egg coat as a protective oocyte envelope
Authors: Killingbeck Emily E. , Wilburn Damien B. , Merrihew Gennifer E. , MacCoss Michael J. , Swanson Willie J. 
Published: June 2021

AbstractAncestrally marine threespine stickleback fish (Gasterosteus aculeatus) have undergone an adaptive radiation into freshwater environments throughout the Northern Hemisphere, creating an excellent model system for studying molecular adaptation and speciation. Ecological and behavioral factors have been suggested to underlie stickleback reproductive isolation and incipient speciation, but reproductive proteins mediating gamete recognition during fertilization have so far remained unexplored. To begin to investigate the contribution of reproductive proteins to stickleback reproductive isolation, we have characterized the stickleback egg coat proteome. We find that stickleback egg coats are comprised of homologs to the zona pellucida (ZP) proteins ZP1 and ZP3, as in other teleost fish. Our molecular evolutionary analyses indicate that across teleosts,ZP3but notZP1has experienced positive Darwinian selection. MammalianZP3is also rapidly evolving, and surprisingly some residues under selection in stickleback and mammalian ZP3 directly align. Despite broad homology, however, we find differences between mammalian and stickleback ZP proteins with respect to glycosylation, disulfide bonding, and sites of synthesis. Taken together, the changes we observe in stickleback ZP protein architecture suggest that the egg coats of stickleback fish, and perhaps fish more generally, have evolved to fulfill a more protective functional role than their mammalian counterparts.
Exertional rhabdomyolysis in newly enrolled cadets of a military academy
Authors: Mao Han‐ding , Li Xin , Liu Shu‐yuan , Xing Ling , Zhao Jin‐bao , Tan Zhi‐jun , Sun Hai‐long , Song Qing 
Published: June 2021

AbstractIntroduction/AimsExertional rhabdomyolysis (ER) often occurs during prolonged intense exercise in hot environments, posing a threat to the health of military personnel. In this study we aimed to investigate possible risk factors for ER and provide further empirical data for prevention and clinical treatment strategies.MethodsA retrospective investigation of 116 concurrent ER cases was conducted. Conditional logistic regression analyses were performed to assess the association between each potential risk (or protective) factor and ER. The clinical characteristics of the 71 hospitalized patients were analyzed descriptively.ResultsAfter screening, the following variables significantly increased the risk of ER: shorter length of service (recruits; odds ratios [OR], 7.49; 95% confidence interval [CI], 2.58‐21.75); higher body mass index (BMI; OR, 1.14, 95% CI, 1.03‐1.26); lack of physical exercise in the last half year (less than once per month; OR, 3.20; 95% CI, 1.08‐9.44); and previous heat injury (OR, 2.94; 95% CI, 1.26‐6.89). Frequent fruit consumption (OR, 0.57; 95% CI, 0.33‐0.99), active hydration habit (OR, 0.37; 95% CI, 0.20‐0.67), water replenishment of more than 2 L on the training day (OR, 0.15; 95% CI, 0.05‐0.45), and water replenishment of at least 500 mL within 1 hour before training (OR, 0.33; 95% CI, 0.12‐0.88) significantly decreased the risk of ER. Of the 71 hospitalized patients, 41 (57.7%) were diagnosed with hypokalemia on admission.DiscussionIn military training, emphasis should be placed on incremental adaptation training before more intense training, and close attention should be given to overweight and previously sedentary recruits. Fluid replenishment before exercise, increased fruit intake, and proper potassium supplementation may help prevent ER.
Kinetics and morphologies in polymerization‐induced cooperative assembly: a computer simulation investigation
Authors: Lu Hui , Song Wen‐Yuan , Zou Ying‐Yi , Xu Wei‐Shao , Yan Yu‐Dou , Liu Hong , Ma Li‐Jun 
Published: June 2021

AbstractIn the last decade, polymerization‐induced self‐assembly (PISA) has emerged to be a quite popular technique for preparing a variety of nanoassembly structures in a selective solvent. As a special technique of PISA, polymerization‐induced cooperative assembly (PICA) is believed to be able to produce more variable nanostructures and its ways of regulation seem more flexible. The process of PICA is studied systematically with a simulation method for discovering possible ways of regulation of morphologies. We find that the existence of solvophobic homopolymers yields a ‘chain growth retarding effect’, which effectively slows down the reaction kinetics of the polymerization. Besides proving the transition from lower‐order morphologies to higher‐order morphologies as reported in experiments, we also find a novel transition from higher‐order morphologies to lower‐order morphologies, as a result of the self‐adaptation behavior of the system. For the first time, we demonstrate that this behavior is able to be well described as a kind of inverse flip‐flop process of vesicle structures to thermodynamically stable micelle structures. We also find that the obvious contrast of length between the solvophilic macro‐chain transfer agents and solvophobic blocks is beneficial to the emergence of plentiful kinds of structures. The results of this study are helpful for understanding the crucial factors for precisely regulating the morphologies in PICA, as well as guiding the way to improve experimental technologies. © 2021 Society of Industrial Chemistry.