bluuebunny/crossref_metadata_2025_split huggingface dataset:
Development and Evaluation of Models to Predict Readmission or Death After Discharge from Intensive Care
Authors: Boyd Jamie M , James Matthew T , Zuege Danny J , Stelfox Henry Thomas 
Published: September 2021

Abstract
Background
Patients being discharged from the intensive care unit (ICU) have variable risks of subsequent readmission or death; however, there is limited understanding of how to predict individual patient risk. We sought to derive risk prediction models for ICU readmission or death after ICU discharge to guide clinician decision-making.
Methods
Systematic review and meta-analysis to identify risk factors. Development and validation of risk prediction models using two retrospective cohorts of patients discharged alive from medical-surgical ICUs (n = 3 ICUs, n = 11,291 patients; n = 14 ICUs, n = 11,400 patients). Models were developed using literature and data-derived weighted coefficients.
Results
Sixteen variables identified from the systematic review were used to develop four risk prediction models. In the validation cohort there were 795 (7%) patients who were re-admitted to ICU and 703 (7%) patients who died after ICU discharge. The area under the curve (AUROC) for ICU readmission for the literature (0.615 [95%CI: 0.593, 0.637]) and data (0.652 [95%CI: 0.631, 0.674]) weighted models showed poor discrimination. The AUROC for death after ICU discharge for the literature (0.708 [95%CI: 0.687, 0.728]) and local data weighted (0.752 [95%CI: 0.733, 0.770]) models showed good discrimination. The negative predictive values for ICU readmission and death after ICU discharge ranged from 94%-98%.
Conclusions
Identifying risk factors and weighting coefficients using systematic review and meta-analysis to develop prediction models is feasible and can identify patients at low risk of ICU readmission or death after ICU discharge.
Development of a Novel CTCs/CTMs Related Prognostic Signature for Hepatocellular Carcinoma
Authors: Li Xiangyu , Shi Yuanxin , Zhao Kai , Lu Yun , Qiu Peng , Deng Zhengdong , Yao Wei , Wang Jianming 
Published: September 2021

Abstract
Background: Hepatocellular carcinoma (HCC) is a common malignancy and the third most deadly cancer worldwide. Previous studies have demonstrated that circulating tumor cells are involved in the occurrence and development of various cancers, including HCC. For this study, we aimed to comprehensively analyze data related to HCC to develop a prognostic model based on CTCs/CTMs related genes (CRGs). Methods: Data were obtained from TCGA, ICGC, and GEO. Firstly, we screened the differentially expressed CRGs and constructed a signature in the TCGA cohort by Lasso‐penalized Cox regression analysis and the multivariate cox regression analysis. Then, the prognostic model was validated in the ICGC dataset and GES14520 dataset with survival analysis and receiver operating characteristic analysis. Moreover, we investigated the clinical significance of prognostic signature, including the correlations with clinical characteristics, immune cell infiltration, and immune checkpoints. Next, we also established the nomogram and to better predict the prognosis of patients. We identified five potential small molecule drugs by Connectivity Map (CMap) and validated them using the Comparative Toxicogenomics Database (CTD). Besides, we further explored the biological role of CDCA8 in hepatocellular carcinoma cells.Results: The prognostic signature exhibited good predictive power and clinical application. Besides, the signature was associated with immune checkpoints (PD-1, PD-L1, and CTLA4), implying that high-risk patients might benefit more from immunotherapy. Additionally, In vitro experiments showed that CDCA8 could promote the proliferation, invasion, and metastasis of hepatocellular carcinoma cells, and silencing CDCA8 could lead to cell cycle arrest and increased apoptosis.Conclusion: We developed a multi-gene classifier that can effectively help the HCC patients benefit from target therapy or immune therapy. And CDCA8 may be the next therapeutic target for hepatocellular carcinoma.
A Long-Term Negative Effect of Monetary Incentives On Participatory Surveillance of Animal Disease
Authors: Kewprasopsak Tossapond , Singhapreecha Charuk , Yano Terdsak , Doluschitz Reiner 
Published: September 2021

Abstract
Background
Generally animal diseases affect widely on human health, therefore practical animal diseases surveillance system is important controlling system to motivate participants in the long term. The objective of this study is to determine the effect of monetary and social motivation on the participatory surveillance of animal diseases. Based on the Fiske’s relational theory (1992), we proposed that there were basically two types of motivation; monetary incentives (monetary markets) and non-monetary incentives (social markets). Our hypothesis is that the effort by monetary motivation is higher than the efforts by social motivation during the payment period. This effort decreases at the end of the payment period, whereas the effort by social motivation is stable.
Methods
We analyzed the data generated by a pilot project that started in 2014 by using a smartphone application to report on the symptoms that indicate animal health malfunctions in Chiang Mai province, northern Thailand. This experiment involved the participation of 67 volunteers from 17 areas in the central part of the province.
Results
The results of the experiment demonstrated that monetary motivation was more effective during the payment period. However, after the termination of the payment period, the social motivation group was more effective. The volunteers given monetary motivation demonstrated not only lower effort than those given social motivation, but the group with monetary motivation was also not re-motivated immediately by social motivation after the payment period had terminated.
Conclusions
In the long run, the social motivation was more efficient and sustainable than the monetary motivation.
Effect of Moisture Content On The Time-Dependent Mechanical Characteristics of Loess
Authors: Chen Lijie , Peng Jianbing , Xie Fei , Leng Yanqiu , Ma Penghui , Nan Jingjing , Yan Ruixin 
Published: September 2021

Abstract
It is of great significance to study the time-dependent mechanical properties of loess, as loess landslides are closely related to them. The purpose of this study is to investigate the effect of moisture content on instantaneous and time-dependent deformation, strength and failure behaviors of undisturbed loess specimens from Nangou in Yan'an City, Shanxi Province, China, via triaxial shearing tests and multi-loading triaxial creep tests under moisture contents of 5%, 10%, 17% and 22%. The results show that the time-dependent deformation of loess increase with the moisture content, while the time-dependent deformation rate decreases slowly. The soil deformation is divided into four stages based on the peak strain rate. Furthermore, the instantaneous and long-term strength of loess decrease with increasing moisture content, and the instantaneous strength decreases more than the long-term strength. The failure mode of undisturbed loess changes from shear failure to homogeneous failure with increasing moisture content; when the failure mode is shear failure, the thickness of the shear band that forms at the specimen surface over time is smaller than the corresponding thickness that forms instantaneously. Finally, the macroscopic morphology and microstructure of loess specimens were considered together to analyze the effect of moisture content on the instantaneous and long-term mechanical behavior of loess and to discuss the process of loess deformation to failure.
Has Iran achieved the goal of reducing the prevalence of households faced with catastrophic health expenditure to 1%? The results of a national study
Authors: Piroozi Bakhtiar , Mahmoodi Hassan , Safari Hossein , Bolbanabad Amjad Mohamadi , Rezaei Satar , Moradi-Joo Mohammad , Shokri Azad 
Published: September 2021

Abstract
Background

Access to universal health coverage and reducing the prevalence of catastrophic health expenditure (CHE) to 1% are the commitments of the Islamic Republic of Iran. The aim of this study was to investigate the prevalence of households exposed to CHE.
Methods

This cross-sectional study was performed on 2000 households in five provinces of Iran in 2021. Data were collected through interviews using the World Health Survey questionnaire.
Results

Data from households whose health care costs were more than 40% of their capacity to pay were included in the group of households with CHE. Determinants of CHE were identified using multivariate regression analysis. 8.3% of households were exposed to CHE. The variables of being a female head of household, use of inpatient, outpatient, dental, and rehabilitation services, families with disabled members and low economic status of the households were significantly associated with increased odds of facing CHE.
Conclusion

In the final year of the sixth five-year development plan, Iran has not yet achieved its goal of "reducing the percentage of households exposed to CHE to 1%," and a high percentage of Iranian households still face CHE. Policymakers should pay attention to factors increasing the chance of facing CHE in designing interventions and this can help the goal of financial protection against health costs.
Vero Cells Gain Renal Tubule Markers in Low-calcium and Magnesium Chemically Defined Media
Authors: Logan Megan , Rinas Karsten , McConkey Brendan , Aucoin Marc G. 
Published: September 2021

Abstract
In this study, a chemically defined, animal component-free media was developed to promote Vero growth in suspension. Key media compounds were screened using Plackett-Burman styled experiments to create a media formulation to support suspension growth. Vero cells remained viable in suspension, but their growth rate was extremely low, conversely, other cell types such as CHO-K1, MDCK and HEK293T were able to grow in single cell suspension in the same media. To investigate the slow growth of Vero cells, RNA-seq analysis was conducted. Vero cells were cultured in three different conditions: adherently in serum-containing medium, adherently in in-house medium, and in suspension in low calcium and magnesium in-house medium. This study illustrates that adherent cells maintain similar gene expression, while the suspension phenotype tends to overexpress genes related to renal tubules.
Associations between a Polymorphism in the rat 5-HT1A Receptor Gene Promoter Region (rs198585630) and Cognitive Alterations Induced by Microwave Exposure
Authors: Li Hai-Juan , Gao Yu , Zou Yong , Qiao Si-Mo , Zhi Wei-Jia , Xu Xin-Ping , Zhou Hong-Mei , Ma Li-Zhen , Wang Lifeng , Hu Xiang-Jun 
Published: September 2021

Abstract
The nervous system is a sensitive target of electromagnetic radiation (EMR). Chronic microwave exposure can induce cognitive deficits, and the 5-HT system is involved in this effect. Genetic polymorphisms lead to individual differences. In this study, we evaluated whether the single-nucleotide polymorphism (SNP) rs198585630 of the 5-HT1A receptor is associated with cognitive alterations in rats after microwave exposure. The transcriptional activity of the 5-HT1A receptor promoter containing the rs198585630 C/T allele was determined in vitro. Electroencephalograms (EEGs), spatial learning and memory, and the mRNA and protein expression of 5-HT1A receptor were evaluated in vivo. We demonstrated that the transcriptional activity of 5-HT1A receptor promoter containing the rs198585630 C allele was higher than that of 5-HT1A receptor promoter containing the T allele. The transcriptional activity of the 5-HT1A receptor promoter was stimulated by 30 mW/cm2 microwave exposure, and the rs198585630 C allele was more sensitive to microwave exposure, as it showed stronger transcriptional activation. Rats carrying the rs198585630 C allele exhibited increased mRNA and protein expression of 5-HT1A receptor and were more susceptible to 30 mW/cm2 microwave exposure, showing cognitive deficits and inhibition of brain electrical activity.
Effect of National Basic Public Health Services On The Health of Internal Migrants in China
Authors: Zhao Liange , yuan hongbin , Wang Xueyuan 
Published: September 2021

Abstract
Objective

This paper evaluates the effect of National Basic Public Health Services (NBPHS) on the health of internal migrants in China.
Study design:

The study design used in this research is a cross-sectional study.
Methods

Data were obtained from the China Migrants Dynamic Survey (CMDS) of 2017, including 150,384 internal migrants at the age of 15–59. Propensity score matching (PSM) was used to estimate the effect of NBPHS.
Results

The findings indicate that NBPHS is successful in improving the health of internal migrants. Different matching algorithms showed the improvement ranging from 2.7 to 2.9 percentage points for the indicator of self-reported health, compared with the reduction of the probability of having disease ranging from 3.3 to 3.7 percentage points in the past year. However, gains are not shared equally. Heterogeneity analysis found significant improvement in the health of patients with hypertension, but the health improvement of diabetics was relatively small. There was also no significant effect on patients with both hypertension and diabetes. Similarly, less improvement was observed in those over 65 years old.
Conclusions

This research suggests that policymakers should not only pay attention to the equalization of project implementation but also focus on narrowing the benefits gap between different groups of internal migrants. This finding highlights the importance of encouraging more young doctors to provide health services in primary institutions and promoting the sinking of high-quality medical resources.
Clinical Efficacy of Endoscopic Technique in the Treatment of Gastrointestinal Anastomotic Complications: a Retrospective Study
Authors: Deng Shenghe , Gu Junnan , Cao Yinghao , Mao Fuwei , Liu Ke , Wang Jiliang , Wu Ke , Cai Kailin 
Published: September 2021

Abstract
Background: To evaluate the safety and effectiveness of endoscopic technique in treating postoperative anastomotic complications of digestive tract. Methods: Clinical data of patients received endoscopic treatment in our hospital due to anastomotic complications after gastrointestinal surgery from January 2015 to December 2018 were collected for retrospective analysis. Endoscopic intervention was used for postoperative anastomotic complications in all the included cases. The time of the intervention measures, laboratory examination, incidence of complications and postoperative follow-up were observed and analyzed.Results: A total of 88 patients were included in the study, including 43 patients with anastomotic stenosis,22 with anastomotic obstruction,23 with anastomotic fistula. For anastomotic obstruction patients, 36 patients with anastomosis were successfully treated with endoscopy. For anastomotic obstruction patients, 18 cases were successfully treated with endoscopy. For anastomotic fistula patients, 21 cases were successfully treated with endoscopy.During follow-up after the endoscopic procedure, 4 patients with anastomotic stenosis needed endoscopic intervention again, 3 cases undertook surgical intervention and 2 cases maintained acceptable defecation function by intermittent dilation with a plastic dilater. one patients with anastomotic obstruction had stent displacement and was removed. For the anastomotic fistula patients,one case relapsed and then underwent endoscopic intervention again, three cases converted to surgical intervention.Preoperative and postoperative blood biochemical examination had no significant statistical significanceConclusion: Endoscopic treatment of anastomosis complications was effective for some of the patients, especially for those with anastomosis stenosis or stricture, considering its saftey, it might be the first choice for anastomosis complications.
Nitrogen enhanced sorghum morpho-physiological activity, and antioxidant capacity
Authors: Ahmad Irshad , Zhu Guanglong , Zhou Guisheng , Song Xudong , Jamal Yousaf , Eldeen Muhi , Gabralla Ebtehal , Hussain Shahid , jun Wangjian 
Published: September 2021

Abstract
Imbalanced mineral nutrition and scant information about nitrogen (N) in plants may result in reduction in sorghum morpho-physiological activities. However, farmers use higher or lower fertilizer doses regarding sorghum growth and yield. This study was undertaken to determine the response of sorghum morpho-physiological activities to different rates of nitrogen (N) during two growing seasons of 2017 and 2018. The treatments were consisted of a factorial combination of three N levels (N1 = 0 kg N ha− 1, N2 = 150 Kg N ha− 1, and N3 = 300 kg N ha− 1) and two varieties (V1 = CFSH30, and V2 = Siyong 3180). Treatments were arranged in a randomized complete block design with three replicates. Our results found that N application (N2 and N3) significantly increased plant growth and morpho-physiological activities; leaf length increased by 2.7–8.9%, leaf width by 0.4–4.5%, plant height by 27.8–20.5%, Specific leaf weight by 4.2–10.9%, leaf weight by 18.4–17.4%, and protein contents by 39.5–117.0% compared to N1. Interestingly, higher rates of N reduced number of plants m− 2 by 42.4–24.8% but increased number of plants m− 2 weight kg− 1 by 11.6–62.8%. Moreover, compared with control, N enhanced CAT activity by 92.8-131.9%, SOD by 81.2–84.5%, and POD by 43.6–52.8% in 2017 and 2018. This study indicated that N3 significantly performed best among all treatments during the two growing seasons particularly in case of V1. Moreover, we also concluded that V1 performed better in terms of producing higher leaf width, plant height, specific leaf weight, stem weight, number of plants m2 (weight/kg− 1), protein contents, and antioxidant enzymes activity as compared to V2.
SIRT7 is a prognostic biomarker in kidney renal clear cell carcinoma that is correlated with immune cell infiltration
Authors: Li Ming , Qian Yue , Mu Lili , Wang Yixian , Jin Xue , Zhang Wei 
Published: September 2021

Abstract
Background: SIRT7 has been shown to be expressed in many cancer types, including kidney renal clear cell carcinoma (KIRC), but its functional role in this oncogenic context remains to be firmly defined. This study was designed to explore correlations between SIRT7 and KIRC characteristics using the TCGA database. Methods: Relationships between SIRT7 expression and KIRC patient clinicopathological characteristics were assessed through Kruskal-Wallis tests, Wilcoxon signed-rank tests, and logistic regression analyses. Area under the ROC curve (AUC) values were used to assess the prognostic value of SIRT7 as a means of classifying KIRC patients. The functional role of SIRT7 in this cancer type was assessed through GO/KEGG enrichment analyses and immune cell infiltration analyses. Results: In KIRC patients, higher levels of SIRT7 expression were associated with Race, M stage, T stage (all P < 0.05). SIRT7 offered significant diagnostic value in ROC curve analyses (AUC = 0.912), and elevated SIRT7 levels were linked to worse patient overall survival (OS; P < 0.001). The expression of SIRT7 was independently related with KIRC patient OS (HR: 1.827; 95%CI: 1.346-2.481; P<0.001). In GO/KEGG analyses, SIRT7 was found to be associated with ubiquitin-mediated proteolysis and nucleotide excision repair. Higher SIRT7 expression was related to the enhanced infiltration of certain immune cells.Conclusions: Increased SIRT7 expression was associated with a worse KIRC patient prognosis, and immune infiltrates, suggesting it may offer value as a prognostic biomarker for this cancer type.
FOXA1 Can Be Modulated by HDAC3 in the Progression of Epithelial Ovarian Carcinoma
Authors: Lou Tong , Liu Chongdong , Qu Hong , Zhang Zhiqiang , Wang Shuzhen , Zhuang Huiyu 
Published: September 2021

Abstract
FOXA1 is associated with malignant tumors, but the function of FOXA1 in EOC is unclear. HDAC3 can influence the proliferation, migration and invasion ability of EOC. In this study, we wanted to explore the function of FOXA1 in ovarian cancer and the relationship between HDAC3 and FOXA1.The expression of HDAC3 and FOXA1 was detected by immunohistochemical staining of primary lesions from 127 epithelial ovarian carcinoma patients. A proliferation assay, a Transwell assay, an apoptosis assay and animal experiments were used to assess the proliferation, invasion and apoptosis abilities of ovarian cancer cells before and after transfection with FOXA1. The relevance of the in vitro findings was confirmed in xenografts. The H-scores for FOXA1 and HDAC3 staining in FIGO stage III-IV were noticeably higher and predicted adverse clinical outcomes in patients with ovarian cancer. The expression level of HDAC3 was significantly correlated with the expression level of FOXA1. Invasion, proliferation and apoptosis capacity and tumor formation were decreased in the FOXA1-knockdown cells. Experiments in xenografts confirmed that HDAC3 mediated tumor formation. In conclusion, FOXA1 can be modulated by HDAC3 through the Wnt/β-catenin signaling pathway, and FOXA1 plays essential roles in the proliferation, apoptosis and invasion of EOC cell lines and xenograft experiments.
Risk factors for emergency department visit in a prospective open cohort of asthmatic children
Authors: Mahut Bruno , Amat Flore , Bokov Plamen , Delclaux Christophe 
Published: September 2021

Abstract
BackgroundRisk factors of emergency department (ED) visits have mainly been obtained from hospital cohorts.ObjectiveTo evaluate risk factors for ED visits in asthmatic children in an out-of-hospital cohort.MethodsWe led a prospective study in an open cohort of 933 asthmatic children followed-up by a specialized pediatrician. We measured the annualized rate of ED visits since age two and described their characteristics at last visit.ResultsMean age (± SD) at last visit was 11.1 ± 3.3 years, and the annualized rate of ED visit was 0.194 ± 0.356. Two groups were defined: one with no ED visits (n = 463), and the other with at least one ED visit (n = 470). The latter group included younger children, with multiple sensitizations and more frequent early atopic dermatitis, who reported having more inhaled corticosteroid (ICS) treatment and a more severe exacerbation rate in the three months prior to the last visit. Socioeconomic status did not influence the rate of ED visits. In a logistic regression, the absence of hospitalization before 2 years of age and of atopic dermatitis had odds ratios of 0.38 (95% confidence interval: 0.23–0.65) and of 0.57 (95% confidence interval: 0.42–0.79) respectively to predict at least one ED visit. When an asthmatic child had no early hospitalization and no atopic dermatitis, the relative risk of ED visit was decreased by 28%.ConclusionAsthmatic children with an absence of atopic dermatitis and hospitalization before two years of age are less prone to emergency department visit after age two.