bluuebunny/crossref_metadata_2025_split huggingface dataset:
Single nucleotide polymorphisms associated with methotrexate-induced nausea in juvenile idiopathic arthritis
Authors: Kyvsgaard Nini , Mikkelsen Torben Stamm , Als Thomas D. , Christensen Anne Estmann , Corydon Thomas J. , Herlin Troels 
Published: April 2021

Abstract
Background

Context: Methotrexate (MTX) is a cornerstone in the treatment of juvenile idiopathic arthritis (JIA). MTX treatment is commonly associated with nausea. Large inter-individual variation exists in the level of MTX-induced nausea, possibly due to genetic factors.

Purpose: To investigate whether MTX-induced nausea was associated with single nucleotide polymorphisms (SNPs) in genes encoding MTX-transporter proteins, a MTX metabolizing enzyme and a nausea receptor.

Findings

Methods: Children aged ≥9 years treated with MTX for JIA were eligible. MTX-induced nausea was registered by the children’s completion of a nausea diary (min. 7 days) and the parents’ completion of the MTX intolerance severity score (MISS). The selected SNPs were: SLCO1B1 (rs4149056; rs4149081), SLCO1B3 (rs2117032), SLC19A1 (rs1051266), ABCC2 (rs2273697; rs3740066; rs717620), ABCB1 (rs2032582; rs1045642), MTHFR (rs1801131, rs1801133), HTR3A (rs1062613; rs1985242; rs1176713) and HTR3B (rs1176744).

Result: Enrolled were 121 JIA patients (82 girls: 39 boys) with a median age of 13.3 years (IQR: 11.3–15.1). The median MTX dose was 9.7 mg/m2/week (IQR: 9.0–10.9). The median MTX treatment duration prior to enrolment was 340 days (IQR: 142–766). The SNP analysis was available for 119 patients. MTX intolerance was associated with the genotype distribution of rs1801133 (MTHFR) (p = 0.02). There was no additive effect of the minor alleles for any of the selected SNPs, nor any significant haplotype associations.

Conclusion

Summary: MTX-induced nausea may be influenced by genetic polymorphisms in a MTX metabolizing enzyme (rs1801133; MTHFR).

Implications: Further analyses involving inclusion of larger cohorts are needed to understand the impact of SNPs on MTX-induced nausea in JIA.
Foreign investment in emerging legal medicinal cannabis markets: the Jamaica case study
Authors: Rychert Marta , Emanuel Machel Anthony , Wilkins Chris 
Published: April 2021

Abstract
Introduction
The establishment of a legal market for medicinal cannabis under the Dangerous Drugs Amendment Act 2015 has positioned Jamaica at the forefront of cannabis law reform in the developing world. Many local cannabis businesses have attracted investment from overseas, including from Canada, US and Europe.

Aim
To explore the opportunities and risks of foreign investment in an emerging domestic legal cannabis market in a developing country.

Methods
Thematic analysis of semi-structured face-to-face interviews with 22 key informants (KIs) from the Jamaican government, local cannabis industry, academia and civil society, and field observations of legal and illegal cannabis cultivators.

Results
KIs from the Jamaican public agencies and domestic cannabis entrepreneurs saw foreign investment as an essential source of capital to finance the start-up costs of legal cannabis businesses. Local cannabis entrepreneurs prioritised investors with the greatest financial resources, brand reputation and export networks. They also considered how allied an investor was with their business vision (e.g., organic cultivation, medical vs. recreational). The key benefits of partnering with a foreign investor included transfer of technical knowledge and financial capital, which enhanced production, quality assurance and seed-to-sale tracking. Some KIs expressed concern over investors’ focus on increasing production efficiency and scale at the expense of funding research and development (R&D) and clinical trials. KIs from the local industry, government agencies and civil society highlighted the risks of ‘predatory’ shareholder agreements and domestic political interference. Concerns were raised about the impact of foreign investment on the diversity of the domestic cannabis sector in Jamaica, including the commitment to transition traditional illegal small-scale cannabis cultivators to the legal sector.

Conclusion
While foreign investment has facilitated the commercialisation of the cannabis sector in Jamaica, regulatory measures are also needed to protect the domestic industry and support the transition of small-scale illegal cultivators to the legal regime. Foreign investments may alter the economic, social and political determinants of health in transitioning from illegal to legal cannabis market economy.
L2MXception: an improved Xception network for classification of peach diseases
Authors: Yao Na , Ni Fuchuan , Wang Ziyan , Luo Jun , Sung Wing-Kin , Luo Chaoxi , Li Guoliang 
Published: April 2021

Abstract
Background
Peach diseases can cause severe yield reduction and decreased quality for peach production. Rapid and accurate detection and identification of peach diseases is of great importance. Deep learning has been applied to detect peach diseases using imaging data. However, peach disease image data is difficult to collect and samples are imbalance. The popular deep networks perform poor for this issue.

Results
This paper proposed an improved Xception network named as L2MXception which ensembles regularization term of L2-norm and mean. With the peach disease image dataset collected, results on seven mainstream deep learning models were compared in details and an improved loss function was integrated with regularization term L2-norm and mean (L2M Loss). Experiments showed that the Xception model with L2M Loss outperformed the current best method for peach disease prediction. Compared to the original Xception model, the validation accuracy of L2MXception was up to 93.85%, increased by 28.48%.

Conclusions
The proposed L2MXception network may have great potential in early identification of peach diseases.
A stable isotope dilution method for a highly accurate analysis of karrikins
Authors: Hrdlička Jakub , Gucký Tomáš , van Staden Johannes , Novák Ondřej , Doležal Karel 
Published: April 2021

Abstract
Background
Karrikins (KARs) are recently described group of plant growth regulators with stimulatory effects on seed germination, seedling growth and crop productivity. So far, an analytical method for the simultaneous targeted profiling of KARs in plant tissues has not been reported.

Results
We present a sensitive method for the determination of two highly biologically active karrikins (KAR1 and KAR2) in minute amounts of plant material (< 20 mg fresh weight). The developed protocol combines the optimized extraction and efficient single-step sample purification with ultra-high performance liquid chromatography-tandem mass spectrometry. Newly synthesized deuterium labelled KAR1 was employed as an internal standard for the validation of KAR quantification using a stable isotope dilution method. The application of the matrix-matched calibration series in combination with the internal standard method yields a high level of accuracy and precision in triplicate, on average bias 3.3% and 2.9% RSD, respectively. The applicability of this analytical approach was confirmed by the successful analysis of karrikins in Arabidopsis seedlings grown on media supplemented with different concentrations of KAR1 and KAR2 (0.1, 1.0 and 10.0 µmol/l).

Conclusions
Our results demonstrate the usage of methodology for routine analyses and for monitoring KARs in complex biological matrices. The proposed method will lead to better understanding of the roles of KARs in plant growth and development.
Forms and functions of bridging factors: specifying the dynamic links between outer and inner contexts during implementation and sustainment
Authors: Lengnick-Hall Rebecca , Stadnick Nicole A. , Dickson Kelsey S. , Moullin Joanna C. , Aarons Gregory A. 
Published: April 2021

AbstractBackgroundBridging factors are relational ties, formal arrangements, and processes that connect outer system and inner organizational contexts. They may be critical drivers of evidence-based practice (EBP) implementation and sustainment. Yet, the complex interplay between outer and inner contexts is often not considered. Bridging factors were recently defined in the updated Exploration, Preparation, Implementation, Sustainment (EPIS) framework. Further identification and specification of this construct will advance implementation models, measures, and methods. Our goal is to advance bridging factor research by identifying relevant dimensions and exemplifying these dimensions through illustrative case studies.MethodsWe used a multiple case study design. Each case (n= 10) represented different contexts, EBPs, and bridging factor types. Inclusion criteria were the presence of clearly distinguishable outer and inner contexts, identifiable bridging factor, sufficient information to describe how the bridging factor affected implementation, and variation from other cases. We used an iterative qualitative inquiry process to develop and refine a list of dimensions. Case data were entered into a matrix. Dimensions comprised the rows and case details comprised the columns. After a review of all cases, we collectively considered and independently coded each dimension as function or form.ResultsWe drew upon the concepts of functions and forms, a distinction originally proposed in the complex health intervention literature. Function dimensions help define the bridging factor and illustrate its purpose as it relates to EBP implementation. Form dimensions describe the specific structures and activities that illustrate why and how the bridging factor has been customized to a local implementation experience. Function dimensions can help researchers and practitioners identify the presence and purpose of bridging factors, whereas form dimensions can help us understand how the bridging factor may be designed or modified to support EBP implementation in a specific context. We propose five function and three form bridging factor dimensions.ConclusionsBridging factors are described in many implementation models and studies, but without explicit reference or investigation. Bridging factors are an understudied and critical construct that requires further attention to facilitate implementation research and practice. We present specific recommendations for a bridging factors research agenda.
Discovery of two novel laccase-like multicopper oxidases from Pleurotus citrinopileatus and their application in phenolic oligomer synthesis
Authors: Zerva A. , Pentari C. , Termentzi A. , America A. H. P. , Zouraris D. , Bhattacharya S. K. , Karantonis A. , Zervakis G. I. , Topakas E. 
Published: April 2021

Abstract
Background
Laccases and laccase-like multicopper oxidases (LMCOs) oxidize a vast array of phenolic compounds and amines, releasing water as a byproduct. Their low substrate specificity is responsible for their tremendous biotechnological interest, since they have been used for numerous applications. However, the laccases characterized so far correspond to only a small fraction of the laccase genes identified in fungal genomes. Therefore, the knowledge regarding the biochemistry and physiological role of minor laccase-like isoforms is still limited.

Results
In the present work, we describe the isolation, purification and characterization of two novel LMCOs, PcLac1 and PcLac2, from Pleurotus citrinopileatus. Both LMCOs were purified with ion-exchange chromatographic methods. PcLac2 was found to oxidize a broader substrate range than PcLac1, but both LMCOs showed similar formal potentials, lower than those reported previously for laccases from white-rot fungi. Proteomic analysis of both proteins revealed their similarity with other well-characterized laccases from Pleurotus strains. Both LMCOs were applied to the oxidation of ferulic and sinapic acid, yielding oligomers with possible antioxidant activity.

Conclusions
Overall, the findings of the present work can offer new insights regarding the biochemistry and variability of low-redox potential laccases of fungal origin. Low-redox potential biocatalysts could offer higher substrate selectivity than their high-redox counterparts, and thus, they could be of applied value in the field of biocatalysis.
Three Babesia species in Ixodes ricinus ticks from migratory birds in Sweden
Authors: Wilhelmsson Peter , Pawełczyk Olga , Jaenson Thomas G. T. , Waldenström Jonas , Olsen Björn , Forsberg Pia , Lindgren Per-Eric 
Published: April 2021

Abstract
Background
Migratory birds can cross geographical and environmental barriers and are thereby able to facilitate transmission of tick-borne pathogens both as carriers of infected ticks and as reservoirs of pathogenic microorganisms. Ixodes ricinus is one of the most abundant tick species in the Northern Hemisphere and a main vector of several Babesia species, some which pose a potential threat to human and animal health. At present only two cases of overt babesiosis in humans have so far been reported in Sweden. To better understand the potential role of birds as disseminators of zoonotic Babesia protozoan parasites, we investigated the presence of Babesia species in ticks removed from migratory birds.

Methods
Ticks were collected from birds captured at Ottenby Bird Observatory, south-eastern Sweden, from March to November 2009. Ticks were molecularly identified to species, and morphologically to developmental stage, and the presence of Babesia protozoan parasites was determined by real-time PCR.

Results
In total, 4601 migratory birds of 65 species were examined for tick infestation. Ticks removed from these birds have previously been investigated for the presence of Borrelia bacteria and the tick-borne encephalitis virus. In the present study, a total of 1102 ticks were available for molecular analysis of Babesia protozoan parasites. We found that 2.4% of the ticks examined, all I. ricinus, were positive for mammal-associated Babesia species. Out of all Babesia-positive samples, Babesia venatorum was the most prevalent (58%) species, followed by Babesia microti (38%) and Babesia capreoli (4.0%). B. venatorum and B. capreoli were detected in I. ricinus larvae, whereas B. microti was only present in I. ricinus nymphs. This supports the view that the two first-mentioned species are vertically (transovarially) transmitted in the tick population, in contrast to B. microti. The largest number of Babesia-infected ticks was removed from the common redstart (Phoenicurus phoenicurus) and European robin (Erithacus rubecula).

Conclusions
This study reveals that Babesia protozoan parasites are present in ticks infesting migratory birds in south-eastern Sweden, which could potentially lead to the dissemination of these tick-borne microorganisms into new areas, thus posing a threat to humans and other mammals.

Graphical Abstract
Generative adversarial network-based rogue device identification using differential constellation trace figure
Authors: Chen Zekun , Peng Linning , Hu Aiqun , Fu Hua 
Published: April 2021

AbstractWith the dramatic development of the internet of things (IoT), security issues such as identity authentication have received serious attention. The radio frequency (RF) fingerprint of IoT device is an inherent feature, which can hardly be imitated. In this paper, we propose a rogue device identification technique via RF fingerprinting using deep learning-based generative adversarial network (GAN). Being different from traditional classification problems in RF fingerprint identifications, this work focuses on unknown accessing device recognition without prior information. A differential constellation trace figure generation process is initially employed to transform RF fingerprint features from time-domain waveforms to two-dimensional figures. Then, by using GAN, which is a kind of unsupervised learning algorithm, we can discriminate rogue devices without any prior information. An experimental verification system is built with 54 ZigBee devices regarded as recognized devices and accessing devices. A universal software radio peripheral receiver is used to capture the signal and identify the accessing devices. Experimental results show that the proposed rogue device identification method can achieve 95% identification accuracy in a real environment.
Colorimetry to assess the visual impact of dust deposition on mosaics at sheltered archaeological sites
Authors: Cabello Briones Cristina , Mayorga Pinilla Santiago , Vázquez Moliní Daniel , Álvarez Fernández-Balbuena Antonio 
Published: April 2021

Abstract
One of the most important alteration factors for archaeological sites is the deposition of dust, primarily onto horizontal surfaces, because it affects both the conservation state of the remains and their appearance. The deposition is responsible for visual changes that prevent proper appreciation of the site, and this is especially negative for the decorative elements such as mosaics. Dust deposition has been seen as a significant problem in sheltered sites as opposed to those located in the open air, where deposits are regularly washed away. However, there is a lack of knowledge on the visual effects of dust on sheltered archaeological remains despite the fact that this information could help to determine cleaning regimes. This research has been undertaken at the House of Hippolytus, a Roman villa located on the outskirts of Complutum, situated where the city of Alcalá de Henares (Spain) stands today. The site was covered with a partially enclosed shelter in 1999 and it contains a magnificent mosaic in the central area. This ornamental floor is the center of attention for visitors and has often been used as a promotional image because of its high artistic value. This study has objectively evaluated the visual changes (color and spectral characteristics) of the mosaic at the House of Hippolytus before and after being cleaned with dry and wet systems using a novel approach based not only on a spectrophotometer (CM-2600d Konica Minolta®) but also on a LumiCam® 1300 camera (Instrument Systems GmbH). Although wet cleaning implies the use of water, which is a decay factor, it has been found to be the best option for recovering the original aspect of the mosaic.
Acute exudative polymorphous paraneoplastic vitelliform maculopathy (AEPPVM) associated with choroidal melanoma
Authors: Gameiro Filho Aluisio Rosa , Sturzeneker Guilherme , Rodriguez Ever Ernesto Caso , Maia André , Morales Melina Correia , Belfort Rubens N. 
Published: April 2021

Abstract
Background
To report a case of acute exudative polymorphous paraneoplastic vitelliform maculopathy in a patient with a history of choroidal melanoma, with metastases to the pancreas, liver, and central nervous system.

Case presentation
A 63-year-old patient, with a history of enucleation of the right eye due to choroidal melanoma, complained of progressive visual loss during a follow-up visit. Fundoscopic examination revealed multiple small areas of serous retinal detachment scattered throughout the posterior pole and ancillary tests confirmed the diagnosis of acute exudative polymorphous paraneoplastic vitelliform maculopathy (AEPPVM). Screening for systemic metastases showed pancreatic, hepatic, and central nervous system involvement.

Conclusions
We describe a rare case of acute exudative polymorphous paraneoplastic vitelliform maculopathy, which should be considered in patients with or without a history of melanoma, who have vitelliform retinal detachments. Nevertheless, no previous reviews of literature have shown a correlation between AEPPVM and pancreatic metastasis.
High-dose radiation associated with improved survival in IDH-wildtype low-grade glioma
Authors: Liu Shuai , Liu Yanwei , Li Guanzhang , Feng Jin , Chen Li , Qiu Xiaoguang 
Published: April 2021

Abstract
Background
As molecular advances have deepened the knowledge on low-grade glioma (LGG), we investigated the effect of higher radiation dose on the survival of IDH-wildtype (IDHwt) LGG.

Methods
In the current study, 52 IDHwt LGG patients who received radiotherapy were enrolled from the Chinese Glioma Genome Atlas dataset. Radiation doses > 54 Gy were defined as high-dose, whereas doses ≤ 54 Gy were defined as low-dose. We performed univariate and multivariate survival analyses to examine the prognostic role of high-dose radiotherapy.

Results
In total, the radiation dose ranged from 48.6 Gy to 61.2 Gy, with a median of 55.8 Gy, and 31 patients were grouped into high-dose radiation. Univariate survival analysis indicated that high-dose radiotherapy (p = 0.015), tumors located in the frontal lobe (p = 0.009), and pathology of astrocytoma (p = 0.037) were significantly prognostic factors for overall survival. In multivariate survival analysis, high-dose radiotherapy (p = 0.028) and tumors located in the frontal lobe (p = 0.016) were independently associated with better overall survival.

Conclusions
In conclusion, high-dose radiotherapy independently improved the survival of IDHwt LGG. This can guide treatments for glioma with known molecular characteristics.
The effects of gender, educational level, and personality on online learning outcomes during the COVID-19 pandemic
Authors: Yu Zhonggen 
Published: April 2021

AbstractWith the rampant pandemic of COVID-19, an increasing number of people are acquiring knowledge through online learning approaches. This study aims to investigate how to improve online learning effectiveness during this special time. Through a mixed design, this study revealed the effect of educational levels, gender, and personality traits on online learning outcomes. It was concluded that postgraduates (N = 599) outperformed undergraduates (N = 553) in online learning, learners (N = 1152) with strong personality traits such as agreeableness, conscientiousness, and openness to a new experience outperformed those with strong extraversion and neuroticism. Future research could improve interpersonal interactions and encourage learners to post words in the online discussion forum, focus on how to design scaffolding online learning and how to improve the quality and dynamic of the online contents, and highlight blended learning rather than either merely online or traditional face-to-face learning.