bluuebunny/crossref_metadata_2025_split huggingface dataset:
Biomimetic Manganese-Eumelanin Nanocomposites for Combined Hyperthermia-Immunotherapy Against Prostate Cancer
Authors: Liu Yu , Shang Wenting , Liu Heng , Hui Hui , Wu Jun , Zhang Wei , Gao Pengli , Guo Kunxiong , Guo Yanli , Tian Jie 
Published: November 2021

Abstract
Pro-tumoral and immunosuppressive M2-like tumor-associated macrophages (TAMs) contribute to tumor progression and distal metastasis. However, current TAMs-modulating therapeutic strategies often encounter challenges including insufficient immune activation, weak antigen presentation ability and unsatisfactory antitumor immune performance. Herein, cyclic RGD peptide functionalized and manganese doped eumelanin-like nanocomposites (RMnMels) were designed for combined hyperthermia-immunotherapy against PC3 prostate cancer. The RMnMels could promote M2-to-M1 macrophage repolarization via scavenging reactive oxygen species and remodel the tumor immunosuppressive microenvironment (TIME). Following near-infrared light irradiation, RMnMels-mediated local hyperthermia not only could destroy tumor cells directly through thermal ablation, but also elicit the release of damage associated molecular patterns and tumor-associated antigens, which could act as effective immunological stimulators to provoke robust tumor immunogenicity and strong antitumor immune responses. The results showed that RMnMels could effectively scavenge reactive oxygen species and promote M2-to-M1 macrophage repolarization both in vitro and in vivo. Synergistically enhanced anti-tumor therapeutic efficacy was achieved following single administration of RMnMels plus single round of laser irradiation, evidenced by decreased primary tumor sizes and decreased number of distant liver metastatic nodules. The as-developed RMnMels may represent a simple and high-performance therapeutic nanoplatform for immunomodulation and enhanced antitumor immune responses.
Ethnobotanical Study of Cowpea (Vigna Unguiculata (L.) Walp.) in Senegal
Authors: Sarr Awa , Bodian Amy , Gueye Mame Codou , Gueye Badara , Kanfani Ghislain , Diatta Cyril , Bougma Ali Lardia , Diop Elisabeth A.M.C. , Cissé Ndiaga , Diouf Diaga , Leclerc Christian 
Published: November 2021

Abstract
Cowpea (Vigna unguiculata) plays a key role in family farming systems in Senegal. It makes an essential contribution to economic, nutritional and food security. Although it is crucial, little is known about how farmers classify the diversity of local varieties or about the social practices associated with them. The aim of this study is to characterize the farming practices associated with growing cowpea in Senegal. Surveys were conducted involving 335 rural farmers living in 37 villages, spread across seven regions that produce cowpea. An average of ten farmers were randomly selected in each village. The results reveal that cowpea is a key feature of cropping systems in the studied area. Our findings highlight the high diversity of local cowpea varieties with 59 local names inventoried. In 75% of cases, the name refers to the seed’s morphology or color. Cowpea production is more diverse in Diourbel and Louga and less diverse in the south. More than half the farmers (57%) acquired their cowpea seeds (early, semi-early and late varieties) outside their village, either from markets, seed suppliers or NGOs. This new understanding of farmers’ expertise in the management of cowpea and its local variability will help to valorise local diversity in breeding programmes.
Coarse-Grained Modeling of The Calcium, Sodium, Magnesium And Potassium Cations Interacting With Proteins
Authors: Lipska Agnieszka Gabriela , Antoniak Anna Maria , Wesołowski Patryk , Warszawski Alan , Samsonov Sergey A , Sieradzan Adam Kazimierz 
Published: November 2021

Abstract
Metal ions play important biological roles e.g.: activation or deactivation of enzymatic reactions and signal transduction. Moreover, they can stabilize protein structure, or even be actively involved in the protein folding process. Therefore, accurate treatment of the ions is crucial to model and investigate biological phenomena properly. In this work the coarse-grained UNRES (UNited RESidue) force field was extended to include the interactions between proteins and four alkali or alkaline earth metal cations of biological significance, i.e. calcium, magnesium, sodium and potassium. Additionally, chloride anions were introduced as counter-ions. Parameters were derived from all-atom simulations and incorporate water in an implicit manner. The new force field was tested on the set of the proteins and was able to reproduce the ion-binding preferences.
Geographical inequalities in COVID-19 mortality: a scoping review protocol
Authors: Bambra Clare , McGowan Victoria J 
Published: November 2021

Abstract
Objective

The objective of this scoping review is to understand what is known in relation to geographical inequalities in COVID-19 mortality.
Introduction:

COVID-19 has occurred against a backdrop of existing social and economic inequalities in health. The impact of the pandemic has been examined across various intersections of health inequalities such as age, gender, ethnicity, and occupation. However, spatial inequalities have been less explored. Therefore, this review aims to identify and synthesise what is known on geographical inequalities in COVID-19 mortality globally.
Inclusion criteria:

Following standard scoping review methodology the inclusion criteria will be guided by Population, Concept, Context (PCC). Population: children and adults (all ages); Concept: Area-level inequalities in COVID-19 mortality; Context: any country, at any level (neighbourhood, town, city, municipality, region). Any published, peer-reviewed study written in English that focuses on all three elements of the PCC inclusion criteria will be included.
Methods

Searches will be conducted in (host sites given in parentheses) Medline (Ovid), Embase (Ovid), Science Citation Index Expanded and Social Science Citation Index (Web of Science) and limited to dates ranging from 1st January 2020 to December 31st 2021. Reference lists of all relevant papers will be searched for additional studies. All titles and abstracts will be screened in Rayyan. Primary studies focusing on COVID-19 mortality rates disaggregated by any measure of area-level deprivation will be selected for full text review. Data relating to study design, population, location, outcomes, and results by deprivation will be extracted using standardised extraction forms. Identified papers meeting the inclusion criteria will be tabulated, synthesised thematically and a narrative constructed to describe the evidence base of geographical inequalities in COVID-19 mortality.
Spatio-temporal analysis of hydropower projects with terrestrial environmentally sensitive areas of Nepal
Authors: Ghimire Hemant R 
Published: November 2021

Abstract
Hydropower project construction is increasing, which can affect the terrestrial environment. Hydropower projects located in environmentally sensitive areas have higher environmental impacts, so I analyzed the spatiotemporal interaction between hydropower project locations and terrestrial environmentally sensitive areas of Nepal to visualize the probable environmental impacts. Most of the existing projects lie on the hill; however, future projects are moving northward. Among the 12 eco-regions of Nepal, hydropower projects are located in 10 eco-regions. Hydropower projects were found to interact with more than half of the biodiverse areas of the country (28 out of 45), and more than five thousand megawatts of hydropower projects are located completely inside these biodiverse areas. The study suggests that the interaction between hydropower projects and environmentally sensitive areas might increase in the future. Hydropower projects should avoid environmentally sensitive areas such as biodiverse areas and protected areas as much as possible to minimize the impacts. Rapid hydropower development is a necessity in countries such as Nepal, so further studies on the effects of hydropower projects on environmentally sensitive areas as well as improvement of the quality of the environmental assessment of the projects are necessary for environmentally friendly development.
Risk Factors for Postoperative Recurrence of Ovarian Endometrioma in Young Women
Authors: Xin Xing , Song Xiaochen , Zhao Xinyang , WU Shanshan , Hao Xinyao , Song Aixin , Meng Zifan , Qi Jiarui , Tan Jichun 
Published: November 2021

Abstract
Purpose

Ovarian endometroma seriously affects women's health, and is susceptible to recurrence after surgery. However, only a few studies have been conducted to asses the risk factors for postoperative recurrence of ovarian endometrioma in young women, and no risk factors associated with recurrence have been found. The purpose of this study is to analyse the risk factors for postoperative recurrence of ovarian endometrioma in young women, and to provide data support for postoperative follow-up of high-risk groups.
Materials and Methods

We retrospectively analysed 196 young women who underwent ovarian endometrioma resection in Peking Union Medical College Hospital and Shengjing Hospital from January 2008 to January 2018 and were followed-up for at least 3 years postoperatively. The patients were divided into a recurrence group or a non-recurrence group. Their preoperative histories, laboratory indexes, intraoperative manifestations, and follow-up symptoms were analysed.
Results

The cumulative recurrence rate of ovarian endometrioma in young women 3 and 5 years after surgery was 8.0%, and 20.3%, respectively. Univariate analysis showed significant differences in cancer antigen-125 levels (hazard ratio [HR]: 3.207, 95% confidence interval [CI]: 1.126–9.135, P = 0.029), the revised American Fertility Society (rAFS) disease stage (HR: 4.588, 95%CI: 1.422–14.805, P = 0.011), postoperative pregnancy (HR: 0.28, 95%CI: 0.109–0.723, P = 0.008), and postoperative dysmenorrhoea (HR: 2.465, 95%CI :1.320–4.604, P = 0.005) between the two groups. Cox multivariate proportional risk analysis showed that rAFS disease stage (HR:3.783, 95%CI: 1.162–12.311, P = 0.027) and postoperative dysmenorrhoea (HR: 2.291, 95%CI :1.222–4.296, P = 0.01) were risk factors for postoperative recurrence of ovarian endometrioma, whereas postoperative pregnancy (HR: 0.361, 95%CI: 0.138–0.944, P = 0.038) was a protective factor for postoperative recurrence. There was no significant correlation between recurrence of ovarian endometrioma and age at surgery, age at the time of menarche, body mass index, duration of dysmenorrhoea, degree of dysmenorrhoea, genital malformation, surgical approach, maximum diameter of the cyst, and postoperative medication.
Conclusion

rAFS disease stage and postoperative dysmenorrhoea are risk factors for the recurrence of ovarian endometrioma during long-term follow-up, whereas postoperative pregnancy is a protective factor.
What is the Impact of CSN5 on the Prognosis of Digestive System Cancers: A Systematic Review and Meta-Analysis
Authors: Guo Yonghua , Gao Meng , Yao Ye , Li Jinghua , Chen Xi , Wang Xingxing , Chen Zhang , Yuan Yufeng , Ma Weijie 
Published: November 2021

Abstract
Background: Despite the understanding of COP9 signalosome subunit 5 (CSN5) in tumor genesis, there is no conclusive evidence on CSN5 value to predict digestive system tumor patients' survival and prognosis. The article was performed to evaluate the impact of CSN5 expression levels on survival consequence and clinicopathological parameters of digestive system neoplasm patients.Methods: A comprehensive search was conducted through four databases. We utilized Hazard Ratio (HR) with a 95% confidence interval (CI) to evaluate the prognostic value of CSN5 in overall survival (OS) and recurrence free survival (RFS). We estimated the connection between CSN5 and clinicopathological parameters based on Odds Ratio (OR) with a corresponding 95% CI. Results: The meta-analysis contained 22 studies, involving 2,193 patients diagnosed with digestive system tumor. High CSN5 expression level was indicated to predict poorer OS (HR = 2.28, 95% CI: 1.71–3.03; p < 0.00001). Additionally, high CSN5 was correlated with worse invasion depth (OR = 0.49, 95% CI: 0.25-0.96, p = 0.04), positive lymphatic metastasis (OR = 0.28, 95% CI: 0.16-0.47, p = 0.00001), positive distant metastasis (OR = 0.32, 95% CI: 0.13-0.76, p = 0.01) and poorer differentiation degree (OR = 0.34, 95% CI: 0.19-0.60, p = 0.0003). However, we could not find a correlation between CSN5 expression and age, gender, tumor stage, tumor size or vascular invasion. Furthermore, no significant publication bias was detected.Conclusion: This meta-analysis demonstrated that the overexpression of CSN5 level could foresee poorer OS in digestive system cancer patients. Additionally, CSN5 level was related to tumor invasion depth, lymphatic metastasis, distant metastasis and differentiation degree.
Identification of Key Iron Metabolism-Related Long Non-Coding Rnas in Hepatocellular Carcinoma Based on Bioinformatics Analysis
Authors: Chen Yu 
Published: November 2021

Abstract
The present study explored the regulatory mechanisms and functional roles of iron metabolism-related long non-coding RNAs (lncRNAs) in hepatocellular carcinoma (HCC) and their potential impact on prognosis of HCC patients. RNA-seq data and clinical information of HCC samples and normal samples were downloaded from The Cancer Genome Atlas (TCGA) database and International Cancer Genome Consortium (ICGC) portal. Iron metabolism-related genes were downloaded from Reactome database and AmiGo2 database. Differential expression and correlation analysis were performed to identify iron metabolism-related differentially expressed lncRNAs (DElncRNAs). Moreover, Kaplan-Meier (KM) survival and receiver operating characteristic (ROC) analysis were used to screen the possible prognostic and diagnostic biomarkers of HCC. A total of 20 differentially expressed and iron metabolism-related genes (DEIMRG) were identified by overlapping 3746 differentially expressed genes (DEGs) and 86 IMRGs. Next, ARHGAP11B, LINC00205, LINC00261 and SNHG12 were screened through Univariate Cox regression. Kaplan-Meier survival curves indicated that ARHGAP11B, LINC00205, LINC00261 and SNHG12 were related to overall survival (OS) in HCC patient in TCGA database. ARHGAP11B, LINC00205 and LINC00261 were finally identified as prognostic DEIMRGs related with OS of HCC patients after validate the survival results in ICGC portal. ARHGAP11B, LINC00205 and LINC00261 all achieved an AUC value of >0.80 in ROC curve analysis. Furthermore, LINC00205 was identified as independently prognostic factor by multivariate Cox analysis combined with clinicopathological factors. Moreover, a ceRNA network including 25 DEmRNAs, 15 DEmiRNA and 3 DElncRNAs was successfully constructed, based on prognostic DElncRNAs and key target miRNAs and mRNAs of them predicted by starBase database and miRwalk. The PPI network illustrated that CDC25A, CHEK1, CCNE2 and ANLN proteins interact more with other proteins. In the present study, we identified iron metabolism related LINC00205 as a prognostic and diagnostic biomarker and constructed a metabolism-related ceRNA network, which may contribute to the treatment of HCC.
An Integrative Multi-Omics Analysis Based on Liquid-Liquid Phase Separation Delineates Distinct Subtypes of Lower-Grade Glioma and Identifies a Prognostic Signature
Authors: Zheng Jianglin , Wu Zhipeng , Qiu Yue , Wang Xuan , Jiang Xiaobing 
Published: November 2021

Abstract
Background: Emerging evidences have indicated that the aberrant liquid-liquid phase separation (LLPS) leads to the dysfunction of biomolecular condensates, thereby contributing to the tumorigenesis and progression. Nevertheless, it remains unclear whether or how the LLPS of specific molecules affects the prognosis and tumor immune microenvironment (TIME) of patients with lower-grade glioma (LGG).Methods: We integrated the transcriptome information of 3585 LLPS-related genes to comprehensively evaluate the LLPS patterns of 423 patients with LGG in The Cancer Genome Atlas (TCGA) cohort. Then, we systematically demonstrated the differences among four LLPS subtypes based on multi-omics analyses. In addition, we constructed the LLPS-related prognostic risk score (LPRS) for individualized integrative assessment. Results: Based on the expression profiles of 85 scaffolds, 355 regulators, and 3145 clients in LGG, we identified four LLPS subtypes, namely LS1, LS2, LS3 and LS4. We confirmed that there were significant differences in prognosis, clinicopathological features, cancer hallmarks, genomic alterations, TIME patterns and immunotherapeutic responses among four LLPS subtypes. In addition, a prognostic signature called LPRS was constructed for individualized integrative assessment. LPRS exhibited a robust predictive capacity for prognosis of LGG patients in multiple cohorts. Moreover, LPRS was found to be correlated with clinicopathological features, cancer hallmarks, genomic alterations and TIME patterns of LGG patients. The predictive power of LPRS in response to immune checkpoint inhibitor (ICI) therapy was also prominent.Conclusions: This study provided a novel classification of LGG patients based on LLPS. The constructed LPRS might facilitate individualized prognosis prediction and better immunotherapy options for LGG patients.
Enhancing the Insecticidal Potential of Baculovirus by Overexpressing the Mammalian Β-Galactosyl Binding Protein Galectin-1
Authors: Lin Chia-Yang , Lin Yu-Chun , Wu YuehLung , Huang Rong-Nan 
Published: November 2021

Abstract
Developing bio-pesticides is an important area of research in agriculture for which viruses are an essential tool. Infection by entomological pathogenic viruses kills agricultural pests, and viral progenies are disseminated to infect more pests, eventually achieving long-term pest control in the field. Of the current virus-based pest control models, Autographa californica multiple nucleopolyhedrovirus (AcMNPV) is the most studied. AcMNPV belongs to the family Baculoviridae and can infect many lepidopterans. Although AcMNPV has been previously demonstrated as a potential pest-control tool, its long virus infection cycle has made field application challenging. To overcome this hurdle, we generated a recombinant baculovirus that can express mammalian galectin-1, which is a-galactoside-binding protein that binds to the peritrophic matrix in the midgut of pest lepidopterans and induces perforation of the membrane. Galectin-1 has been shown to affect immune responses in mammals, including humans, but to our knowledge, the effect of galectin-1 on insect immune systems has not been previously reported. Hosts infected with this recombinant virus exhibited reduced appetite and died sooner in both laboratory and small-scale field studies, suggesting that the overexpression of galectin-1 can more efficiently eliminate pest hosts. In addition to disrupting the integrity of the peritrophic matrix, the immune system of hosts infected with recombinant baculovirus carrying the galectin-1 gene was suppressed, making hosts more vulnerable to secondary infection. These results demonstrated that the potential of baculovirus for pest control can be improved by using a recombinant baculovirus that overexpresses mammalian galectin-1 in hosts.
Convergence of Energy Carbon Emission Efficiency: Evidence From Manufacturing Sub-sectors in China
Authors: Liu Dongdong 
Published: November 2021

Abstract
With China's economy entering the stage of high-quality development, manufacturing energy carbon emission efficiency has become the focus of academic attention. Based on the panel data of China's manufacturing sub-sectors, this paper measures and analyzes the evolution trend of manufacturing energy carbon emission and its efficiency. On this basis, this paper uses coefficient of variation and convergence model to test the convergence of manufacturing energy carbon emission efficiency. The results show that China’s manufacturing energy carbon emissions and its efficiency show an increasing trend. The coal was the main source of manufacturing energy carbon emissions. The manufacturing energy carbon emission efficiency does not exist σ convergence, but exists β convergence, and its convergence exists industry heterogeneity. The manufacturing energy carbon emission efficiency exits scale effect and technology effect, but not the effect of opening to the outside world and institutional effect, and its effect exists industry heterogeneity. By reducing carbon emissions, adopting differentiated policies, adjusting the industry scale, and enhancing the industry technology intensity, China's manufacturing can improve the energy carbon emissions efficiency and promote high-quality economic development.
Simulation and Optimization of Airport Rainwater Drainage System at Different Control Measures
Authors: Peng Jing , Yu Lei , Zhong Xiang , Dong Tiansong 
Published: November 2021

Abstract
The impervious area of the airport is high, which leads to the deterioration of the water environment and frequent waterlogging disasters. The construction of sponge airport has become an important and arduous task in the new era of civil aviation design industry in China. In order to compare the effects of different control measures at different scenarios, take the airport along China's southeast coast as an example, three scenarios were designed in this study (Scenario 1: no LID facilities and other measures; Scenario 2: two pump stations were setting; Scenario 3: both LID facilities and pump stations). Three simulation models under LID facilities and other measures were developed using SWMM with return period of 5a. The simulation results at different scenarios were compared, the number and the best opening scheme of pumps for each reservoir are finally obtained. The results of Scenario 3 show that the full-flow duration of nodes in the study area is greatly shortened. The decrease of full-flow duration of J1, J2 and J3 was 1.2, 0.8 and 0.5 hours respectively, with reduction rates of 40%, 53.3% and 28.6% respectively. The rainfall peak flows both the first and the second were reduced in this scenario, and the reduction rates were 10.68% and 12.78% respectively. However, the reduction effect of the third peak is poor with the further increase of rainfall intensity. The reduction rate of the total inflow and peak flow of rainwater buckets and permeable pavement is better than that of vegetative swale. The results of this study can provide the reference for the design of sponge airport and the airport flood control management.