bluuebunny/crossref_metadata_2025_split huggingface dataset:
Scale-up fermentation of Escherichia coli for the production of recombinant endoglucanase from Clostridium thermocellum
Authors: Shahzadi Iram , Al-Ghamdi Maryam A. , Nadeem Muhammad Shahid , Sajjad Muhammad , Ali Asif , Khan Jalaluddin Azam , Kazmi Imran 
Published: March 2021

AbstractEndoglucanase (EC 3.2.1.4) catalysing the hydrolysis of β-1.4-glycosidic linkage of cellulose molecules is an enzyme of tremendous industrial importance. The present study describes a response surface methodology based predicted model to deduce a set of fermentation conditions for optimum growth and activity of recombinant endoglucanase in E. coli BL21 (DE3). Numerous significant parameters including fermentation media composition, temperature (Celsius), pH and agitation rate (rpm) were analysed systemically by employing central composite design. This effort reports highly efficient recombinant endoglucanase overproduction (6.9 gl−1 of biomass) with 30% expression by E. coli in modified M9NG media incubated at 37 °C and pH 7 agitated at 200 rpm. Addition of 3 mM glucose and 24 mM glycerol in the M9NG media has shown positive effect on the enzyme yield and activity. The CMCase activity experimentally estimated was found to be 1185 U/mg with the optimized parameters. The outcomes of both the responses by the predicted quadratic model were found in consensus with the obtained values. Our results well depicted the favourable conditions to further scale-up the volumetric yield of other relevant recombinant enzymes and proteins.
Cerebrospinal fluid findings in patients with psychotic symptoms—a retrospective analysis
Authors: Rattay Tim W. , Martin Pascal , Vittore Debora , Hengel Holger , Cebi Idil , Tünnerhoff Johannes , Stefanou Maria-Ioanna , Hoffmann Jonatan F. , von der Ehe Katrin , Klaus Johannes , Vonderschmitt Julia , Herrmann Matthias L. , Bombach Paula , Al Barazi Hazar , Zeltner Lena , Richter Janina , Hesse Klaus , Eckstein Kathrin N. , Klingberg Stefan , Wildgruber Dirk 
Published: March 2021

AbstractIn current international classification systems (ICD-10, DSM5), the diagnostic criteria for psychotic disorders (e.g. schizophrenia and schizoaffective disorder) are based on symptomatic descriptions since no unambiguous biomarkers are known to date. However, when underlying causes of psychotic symptoms, like inflammation, ischemia, or tumor affecting the neural tissue can be identified, a different classification is used ("psychotic disorder with delusions due to known physiological condition" (ICD-10: F06.2) or psychosis caused by medical factors (DSM5)). While CSF analysis still is considered optional in current diagnostic guidelines for psychotic disorders, CSF biomarkers could help to identify known physiological conditions. In this retrospective, partly descriptive analysis of 144 patients with psychotic symptoms and available CSF data, we analyzed CSF examinations' significance to differentiate patients with specific etiological factors (F06.2) from patients with schizophrenia, schizotypal, delusional, and other non-mood psychotic disorders (F2). In 40.3% of all patients, at least one CSF parameter was out of the reference range. Abnormal CSF-findings were found significantly more often in patients diagnosed with F06.2 (88.2%) as compared to patients diagnosed with F2 (23.8%, p < 0.00001). A total of 17 cases were identified as probably caused by specific etiological factors (F06.2), of which ten cases fulfilled the criteria for a probable autoimmune psychosis linked to the following autoantibodies: amphiphysin, CASPR2, CV2, LGl1, NMDA, zic4, and titin. Two cases presented with anti-thyroid tissue autoantibodies. In four cases, further probable causal factors were identified: COVID-19, a frontal intracranial tumor, multiple sclerosis (n = 2), and neurosyphilis. Twenty-one cases remained with "no reliable diagnostic classification". Age at onset of psychotic symptoms differed between patients diagnosed with F2 and F06.2 (p = 0.014), with the latter group being older (median: 44 vs. 28 years). Various CSF parameters were analyzed in an exploratory analysis, identifying pleocytosis and oligoclonal bands (OCBs) as discriminators (F06.2 vs. F2) with a high specificity of > 96% each. No group differences were found for gender, characteristics of psychotic symptoms, substance dependency, or family history. This study emphasizes the great importance of a detailed diagnostic workup in diagnosing psychotic disorders, including CSF analysis, to detect possible underlying pathologies and improve treatment decisions.
Assessing adaptive requirements and breeding potential of spelt under Mediterranean environment
Authors: Curzon Arie Y. , Kottakota Chandrasekhar , Nashef Kamal , Abbo Shahal , Bonfil David J. , Reifen Ram , Bar-El Shimrit , Rabinovich On , Avneri Asaf , Ben-David Roi 
Published: March 2021

AbstractThe rising demand for spelt wheat (Triticum aestivum ssp. spelta) as a high-value grain crop has raised interest in its introduction into non-traditional spelt growing areas. This study aimed to assess adaptive constrains of spelt under short Mediterranean season. At first screening of a wide spelt collection for phenology and allelic distribution at the photoperiod (PPD) and vernalization (VRN) loci was done. In addition an in-depth phenotypic evaluation of a selected panel (n = 20) was performed, including agronomically important traits and concentration of grain mineral (GMC) and grain protein (GPC) content. Results from both wide screening and in-depth in panel (group of 18 spelt lines and two bread wheat lines) evaluation shows that the major adaptive constraint for spelt under Mediterranean conditions is late heading, caused by day length sensitivity, as evident from phenology and allelic profile (PPD and VRN). All lines carrying the photoperiod-sensitive allele (PPD-D1b) were late flowering (> 120DH). Based on the panel field evaluations those consequently suffer from low grain yield and poor agronomic performances. As for minerals, GMC for all but Zn, significantly correlated with GPC. In general, GMC negatively correlated with yield which complicated the assessment of GMC per-se and challenge the claim for higher mineral content in spelt grains. The exceptions were, Fe and Zn, which did not correlate with yield. Spelt lines showing high Fe and Zn concentration in a high-yield background illustrate their potential for spelt wheat breeding. Improving spelt adaptation to Mediterranean environments could be mediated by introducing the insensitive-PPD-D1a allele to spelt wheat background. Following this breeding path spelt could better compete with bread wheat under short season with limited and fluctuating rain fall.
Gastrectomy for stage IV gastric cancer: a comparison of different treatment strategies from the SEER database
Authors: Desiderio Jacopo , Sagnotta Andrea , Terrenato Irene , Annibale Bruno , Trastulli Stefano , Tozzi Federico , D’Andrea Vito , Bracarda Sergio , Garofoli Eleonora , Fong Yuman , Woo Yanghee , Parisi Amilcare 
Published: March 2021

AbstractIn the West, more than one third of newly diagnosed subjects show metastatic disease in gastric cancer (mGC) with few care options available. Gastrectomy has recently become a subject of debate, with some evidence showing advantages in survival beyond the sole purpose of treatment tumor-related complications. We investigated the survival benefit of different strategies in mGC patients, focusing on the role and timing of gastrectomy. Data were extracted from the SEER database. Groups were determined according to whether patients received gastrectomy, chemotherapy, supportive care. Patients receiving a multimodality treatment were further divided according to timing of surgery, whether performed before (primary gastrectomy, PG) or after chemotherapy (secondary gastrectomy, SG). 16,596 patients were included. Median OS was significantly higher (p < 0.001) in the SG (15 months) than in the PG (13 months), gastrectomy alone (6 months), and chemotherapy (7 months) groups. In the multivariate analysis, SG showed better OS (HR = 0.22, 95%CI = 0.18–0.26, p < 0.001) than PG (HR = 0.25, 95%CI = 0.23–0.28, p < 0.001), gastrectomy (HR = 0.40, 95%CI = 0.36–0.44, p < 0.001), and chemotherapy (HR = 0.42, 95%CI = 0.4–0.44, p < 0.001). The survival benefits persisted even after the PSM analysis. This study shows survival advantages of gastrectomy as multimodality strategy after chemotherapy. In selected patients, SG can be proposed to improve the management of stage IV disease.
Left posterior temporal cortex is sensitive to syntax within conceptually matched Arabic expressions
Authors: Matar Suhail , Dirani Julien , Marantz Alec , Pylkkänen Liina 
Published: March 2021

AbstractDuring language comprehension, the brain processes not only word meanings, but also the grammatical structure—the “syntax”—that strings words into phrases and sentences. Yet the neural basis of syntax remains contentious, partly due to the elusiveness of experimental designs that vary structure independently of meaning-related variables. Here, we exploit Arabic’s grammatical properties, which enable such a design. We collected magnetoencephalography (MEG) data while participants read the same noun-adjective expressions with zero, one, or two contiguously-written definite articles (e.g., ‘chair purple’; ‘the-chair purple’; ‘the-chair the-purple’), representing equivalent concepts, but with different levels of syntactic complexity (respectively, indefinite phrases: ‘a purple chair’; sentences: ‘The chair is purple.’; definite phrases: ‘the purple chair’). We expected regions processing syntax to respond differently to simple versus complex structures. Single-word controls (‘chair’/‘purple’) addressed definiteness-based accounts. In noun-adjective expressions, syntactic complexity only modulated activity in the left posterior temporal lobe (LPTL), ~ 300 ms after each word’s onset: indefinite phrases induced more MEG-measured positive activity. The effects disappeared in single-word tokens, ruling out non-syntactic interpretations. In contrast, left anterior temporal lobe (LATL) activation was driven by meaning. Overall, the results support models implicating the LPTL in structure building and the LATL in early stages of conceptual combination.
The impact of viremia on organ failure, biomarkers and mortality in a Swedish cohort of critically ill COVID-19 patients
Authors: Järhult Josef D. , Hultström Michael , Bergqvist Anders , Frithiof Robert , Lipcsey Miklos 
Published: March 2021

AbstractThe spread of virus via the blood stream has been suggested to contribute to extra-pulmonary organ failure in Coronavirus disease 2019 (COVID-19). We assessed SARS-CoV-2 RNAemia (RNAemia) and the association between RNAemia and inflammation, organ failure and mortality in critically ill COVID-19 patients. We included all patients with PCR verified COVID-19 and consent admitted to ICU. SARS-CoV-2 RNA copies above 1000/ml measured by PCR in plasma was defined as RNAemia and used as surrogate for viremia. In this cohort of 92 patients 59 (64%) were invasively ventilated. RNAemia was found in 31 patients (34%). Hypertension and corticosteroid treatment was more common in patients with RNAemia. Extra-pulmonary organ failure biomarkers and the extent of organ failure were similar in patients with and without RNAemia, but the former group had more renal replacement therapy and higher mortality (26 vs 16%; 35 vs 16%, respectively, p = 0.04). RNAemia was not an independent predictor of death at 30 days after adjustment for age. SARS-CoV2 RNA copies in plasma is a common finding in ICU patients with COVID-19. Although viremia was not associated with extra pulmonary organ failure it was more common in patients who did not survive to 30 days after ICU admission.Trial registration: ClinicalTrials NCT04316884.
Comparison of three different types of exercises for selective contractions of supra- and infrahyoid muscles
Authors: Chang Min Cheol , Park Sungwon , Cho Joo Young , Lee Byung Joo , Hwang Jong-Moon , Kim KwanMyung , Park Donghwi 
Published: March 2021

AbstractSeveral exercise methods, such as the Shaker exercise, tongue press exercise, chin tuck against resistance (CTAR) exercise, and submandibular push exercise, have been introduced to strengthen the muscles involved in swallowing. In this study, we compared the effectiveness of the CTAR, submandibular push, and Shaker exercises for the induction of selective supra- and infrahyoid muscle contractions using surface electromyography (EMG). This study is a prospective non-randomized controlled study. Twenty-five healthy subjects and 20 patients experiencing swallowing difficulty were enrolled. During the three different types of exercises, the root mean square (RMS) values of the sternocleidomastoid (SCM), suprahyoid (anterior belly of the digastric and mylohyoid muscles), and infrahyoid (sternothyroid and thyrohyoid muscles) muscles were analyzed using surface EMG. Differences in the activity of swallowing muscles among the three different exercises were analyzed using one-way repeated measured analysis of variance. In terms of both the maximum and mean RMS values of the suprahyoid muscle, the submandibular push exercise showed a larger RMS value than the CTAR and Shaker exercises in healthy subjects (p < 0.05). In terms of both the maximum and mean RMS values of the suprahyoid muscle, the Shaker exercise and submandibular push exercise showed a larger RMS value than the CTAR exercise in patients with swallowing difficulty (p < 0.05). The submandibular push exercise may be effective as a swallowing muscle exercise owing to its superiority in inducing selective contractions of the supra- and infrahyoid muscles. The CTAR and Shaker exercises are also effective in this regard.
Statistics of seismicity to investigate the Campi Flegrei caldera unrest
Authors: Tramelli A. , Godano C. , Ricciolino P. , Giudicepietro F. , Caliro S. , Orazi M. , De Martino P. , Chiodini G. 
Published: March 2021

AbstractThe knowledge of the dynamic of the Campi Flegrei calderic system is a primary goal to mitigate the volcanic risk in one of the most densely populated volcanic areas in the world. From 1950 to 1990 Campi Flegrei suffered three bradyseismic crises with a total uplift of 4.3 m. After 20 years of subsidence, the uplift started again in 2005 accompained by a low increment of the seismicity rate. In 2012 an increment in the seismic energy release and a variation in the gas composition of the fumaroles of Solfatara (in the central area of the caldera) were recorded. Since then, a slow and progressive increase in phenomena continued until today. We analyze the INGV - Osservatorio Vesuviano seismic catalogue of Campi Flegrei from 2000 to 2020 in order to look for any variation in the seismic parameters and compare them with geochemical monitored ones. A remarkable correlation between independent variables of earthquake cumulative number, CO/CO2 values and vertical ground deformation reveals a likely common origin. Moreover the correlation between all the variables here analysed enlightens that the same origin can cause the temporal behavior of all these variables. We interpret the seismological, geochemical and geodetic observable in terms of the injection of magmatic fluids into the hydrothermal system or its pressurization.
Near-surface real-time seismic imaging using parsimonious interferometry
Authors: Hanafy Sherif M. , Hoteit Hussein , Li Jing , Schuster Gerard T. 
Published: March 2021

AbstractResults are presented for real-time seismic imaging of subsurface fluid flow by parsimonious refraction and surface-wave interferometry. Each subsurface velocity image inverted from time-lapse seismic data only requires several minutes of recording time, which is less than the time-scale of the fluid-induced changes in the rock properties. In this sense this is real-time imaging. The images are P-velocity tomograms inverted from the first-arrival times and the S-velocity tomograms inverted from dispersion curves. Compared to conventional seismic imaging, parsimonious interferometry reduces the recording time and increases the temporal resolution of time-lapse seismic images by more than an order-of-magnitude. In our seismic experiment, we recorded 90 sparse data sets over 4.5 h while injecting 12-tons of water into a sand dune. Results show that the percolation of water is mostly along layered boundaries down to a depth of a few meters, which is consistent with our 3D computational fluid flow simulations and laboratory experiments. The significance of parsimonious interferometry is that it provides more than an order-of-magnitude increase of temporal resolution in time-lapse seismic imaging. We believe that real-time seismic imaging will have important applications for non-destructive characterization in environmental, biomedical, and subsurface imaging.
Underrated primary biogenic origin and lifetime of atmospheric formic and acetic acid
Authors: Lee Xinqing , Huang Daikuan , Liu Qi , Liu Xueyan , Zhou Hui , Wang Qian , Ma Yuena 
Published: March 2021

AbstractFormic and acetic acids are ubiquitous in the troposphere, playing an important role in the atmospheric chemistry. Recent model studies ended up with substantial low bias on their tropospheric budgets presumably due to a large missing biogenic source derived most likely from photochemical oxidation of long-lived volatile organic compound(s), i.e., a secondary biogenic emission. Here, by studying the stable carbon isotope composition of formic and acetic acid in couple in the troposphere and relevant sources, we find the gap relates to primary biogenic emission and atmospheric lifetime of the acids. We show the primary biogenic emission is only second to the secondary biogenic emission as a strong source. Marine emission is the least one yet present in all the tropospheric environments except some local air. Long-distance transport of this origin indicates the lifetime over 5 days for both acids. Our results indicate that recent simulations underrated both primary biogenic emission and the lifetime. These underestimations would inevitably bias low the modeled results, especially in the low and free troposphere where primary biogenic emission and lifetime has the most pronounced influence, respectively.
Discovery of novel antituberculosis agents among 3-phenyl-5-(1-phenyl-1H-[1,2,3]triazol-4-yl)-[1,2,4]oxadiazole derivatives targeting aminoacyl-tRNA synthetases
Authors: Rybak Mariia Yu. , Balanda Anatoliy O. , Yatsyshyna Anna P. , Kotey Igor. M. , Starosyla Sergiy A. , Bdzhola Volodymyr G. , Lukash Lubov L. , Yarmoluk Sergiy M. , Tukalo Michael A. , Volynets Galyna P. 
Published: March 2021

AbstractAntibiotic resistance is a major problem of tuberculosis treatment. This provides the stimulus for the search of novel molecular targets and approaches to reduce or forestall resistance emergence inMycobacterium tuberculosis. Earlier, we discovered a novel small-molecular inhibitor among 3-phenyl-5-(1-phenyl-1H-[1,2,3]triazol-4-yl)-[1,2,4]oxadiazoles targeting simultaneously two enzymes—mycobacterial leucyl-tRNA synthetase (LeuRS) and methionyl-tRNA synthetase (MetRS), which are promising molecular targets for antibiotic development. Unfortunately, the identified inhibitor does not reveal antibacterial activity towardM. tuberculosis. This study aims to develop novel aminoacyl-tRNA synthetase inhibitors among this chemical class with antibacterial activity toward resistant strains ofM. tuberculosis. We performed molecular docking of the library of 3-phenyl-5-(1-phenyl-1H-[1,2,3]triazol-4-yl)-[1,2,4]oxadiazole derivatives and selected 41 compounds for investigation of their inhibitory activity toward MetRS and LeuRS in aminoacylation assay and antibacterial activity towardM. tuberculosisstrains using microdilution assay. In vitro screening resulted in 10 compounds active against MetRS and 3 compounds active against LeuRS. Structure-related relationships (SAR) were established. The antibacterial screening revealed 4 compounds active towardM. tuberculosismono-resistant strains in the range of concentrations 2–20 mg/L. Among these compounds, only one compound27has significant enzyme inhibitory activity toward mycobacterial MetRS (IC50 = 148.5 µM). The MIC for this compound towardM. tuberculosisH37Rv strain is 12.5 µM. This compound is not cytotoxic to human HEK293 and HepG2 cell lines. Therefore, 3-phenyl-5-(1-phenyl-1H-[1,2,3]triazol-4-yl)-[1,2,4]oxadiazole derivatives can be used for further chemical optimization and biological research to find non-toxic antituberculosis agents with a novel mechanism of action.
New insights into the molecular mechanism behind mannitol and erythritol fructosylation by β-fructofuranosidase from Schwanniomyces occidentalis
Authors: Rodrigo-Frutos David , Jiménez-Ortega Elena , Piedrabuena David , Ramírez-Escudero Mercedes , Míguez Noa , Plou Francisco J. , Sanz-Aparicio Julia , Fernández-Lobato María 
Published: March 2021

AbstractThe β-fructofuranosidase from Schwanniomyces occidentalis (Ffase) is a useful biotechnological tool for the fructosylation of different acceptors to produce fructooligosaccharides (FOS) and fructo-conjugates. In this work, the structural determinants of Ffase involved in the transfructosylating reaction of the alditols mannitol and erythritol have been studied in detail. Complexes with fructosyl-erythritol or sucrose were analyzed by crystallography and the effect of mutational changes in positions Gln-176, Gln-228, and Asn-254 studied to explore their role in modulating this biocatalytic process. Interestingly, N254T variant enhanced the wild-type protein production of fructosyl-erythritol and FOS by $$\sim$$
∼
30% and 48%, respectively. Moreover, it produced neokestose, which represented $$\sim$$
∼
27% of total FOS, and yielded 31.8 g l−1 blastose by using glucose as exclusive fructosyl-acceptor. Noteworthy, N254D and Q176E replacements turned the specificity of Ffase transferase activity towards the synthesis of the fructosylated polyols at the expense of FOS production, but without increasing the total reaction efficiency. The results presented here highlight the relevance of the pair Gln-228/Asn-254 for Ffase donor-sucrose binding and opens new windows of opportunity for optimizing the generation of fructosyl-derivatives by this enzyme enhancing its biotechnological applicability.
Uncertainty about the risks associated with microplastics among lay and topic-experienced respondents
Authors: Thiele Christina J. , Hudson Malcolm D. 
Published: March 2021

AbstractMicroplastics are contaminants of emerging concern but there is currently a lack of evidence on actual risks relating to them, despite claims in media and scientific articles. Research on people’s perceptions on microplastics is in its infancy. Here we present part of a larger survey about people’s perceptions of issues with microplastics. Our analysis of 1681 responses across the globe to an online questionnaire demonstrates a certain level of uncertainty, not only in lay people but also respondents who study/work on the topic of plastics and microplastics as a pollutant. This uncertainty ranges from level of concern about microplastics as an environmental issue to existing evidence for effects. Further, there is some discrepancy between risk perception and state of the research. Some of this may be driven by scientific work with some serious limitations in reporting and methods. This highlights the need for fact-checking of circulating information about microplastics, but also for addressing the discordance between ecotoxicological risk and how risk is framed within the scientific community.