bluuebunny/crossref_metadata_2025_split huggingface dataset:
Urbanidade e agricultura no contemporâneo: pensando a alimentação pelo viés da agroecologia e as relações com uma dinâmica socioambiental
Authors: Folmer Ivanio , Machado Gabriella Eldereti 
Published: February 2021

RESUMO: A agroecologia se dispõe a propor e discutir o modelo convencional de agricultura atual e consequentemente o modelo de produção de alimentos, ressaltando alternativas de mudança para estilos de agriculturas de base ecológica. Compreende um enfoque teórico e metodológico multidisciplinar visando à sustentabilidade ecológica, social, econômica, cultural, política e ética para uma produção de alimentos saudáveis. Os diálogos que a Agroecologia propõe vão desde o conhecimento dos agricultores sobre as plantas, sobre os diferentes tipos de solos, os processos ecológicos, conhecimentos sobre o ambiente em geral, todos esses saberes são importantes para se propor uma dinâmica socioambiental de sociedade. Nesse contexto, para permear o diálogo que a agroecologia trás para o urbano, buscando caracterizar a urbanidade contemporânea e seu potencial de criação do novo, de outros espaços para a agricultura, como na agricultura urbana, que vem crescendo muito nos últimos anos. O objetivo deste trabalho é propor a problematização da urbanidade e da agricultura no cenário atual, sendo feito por meio de uma pesquisa teórica, no qual serão discutidos alguns conceitos base para o entendimento dessas relações. Iremos discutir as questões conceituais envolvendo o território, urbanidade, agroecologia e a produção de alimentos no contemporâneo. Além da abordagem das Políticas Públicas com enfoque na produção de alimentos saudáveis, contemplando um ambiente socioambiental equilibrado.
PALAVRAS-CHAVE: Agroecologia; Socioambiental; Meio Ambiente.
Desenvolvimento de um material educativo com aplicabilidade na temática socioambiental: uma abordagem ligada aos profissionais do turismo
Authors: Melo Maria Carolina Acioli de , Kozlowiski Vanessa Doro Abdallah 
Published: February 2021

RESUMO: Apesar de uma constante preocupação com o meio ambiente, onde se fala diariamente sobre sustentabilidade, existe um contrassenso, onde se vê o descarte incorreto de óleo de origem vegetal ou animal proveniente de frituras, tanto no campo residencial, quando comercial, diretamente no sistema de esgoto, no solo e até mesmo no lixo. As consequências destes atos são diversos problemas, como o entupimento dos encanamentos, impermeabilização do solo, encarecimento do tratamento na rede de esgoto, dentre outros. O presente trabalho teve como principal objetivo desenvolver um material educativo voltado para a temática ambiental, com foco nos profissionais do turismo, elaborando, discutiu-se também a importância e a aplicabilidade da Logística Reversa para o problema do descarte incorreto do óleo de cozinha usado, bem como o impacto deste para o meio ambiente, por intermédio da aplicação de um questionário, que visou estabelecer uma percepção dos participantes da pesquisa em relação as consequências, oferendo uma visão real sobre o descarte deste resíduos sólido. Não obstante, ocorra tanta discussão sobre o assunto em escolas, universidades e mídia, durante a aplicação do questionário, verificou-se que nem todos os entrevistados sabiam a forma correta do descarte e também dos seus malefícios para o meio ambiente. O questionário fora aplicado em dois hotéis, ambos no município de Maceió, foram 19 participantes da pesquisa, sendo 10 funcionários de um Resort e nove funcionários de um Hotel. Para a devida apreciação dos resultados, os funcionários dos hotéis foram divididos em dois grupos, nomeados como colaboradores dos serviços gerais e colaboradores administrativos, sendo o primeiro composto por funcionários dos serviços gerais, funcionários da cozinha e funcionários da recepção e o segundo grupo composto por chefes de cozinha, responsáveis pela cozinha e gerência do hotel. Diante do apanhado, foi notório que no primeiro grupo, a maioria das respostas foi “sim”, revelando que estes tinham conhecimento acerca das questões referentes ao uso e descarte do óleo de cozinha, onde mostraram interesse sobre o desenvolvimento de uma cartilha sobre o ensino do descarte adequado do óleo, assim como o que pode ser feito com este resíduo já utilizado.
PALAVRAS-CHAVE: óleo de cozinha, logística reversa, Política Nacional de Resíduos sólidos.
Improvement of Microbial Cell Concentration Technology in the Production of Live Plague Vaccine in the Form of Orodispersible Tablets
Authors: Sharov D. A. , Leshchenko A. A. , Bagin S. V. , Logvinov S. V. , Mokhov D. A. , Ezhov A. V. , Lazykin A. G. , Krupin V. V. , Kosenkov I. V. 
Published: February 2021

The aim of the study was to improve the procedure for the Yersinia pestis EV strain cell concentration using the system for tangential flow microfiltration with the ASF-020 filter support unit.Materials and methods. The study used the vaccine Y. pestis EV strain derived from NIIEG cell line. Submerged cultivation of the native culture was performed using BIOR-0.25 reactor with automated control system. Microbial suspension concentrate was produced through microfiltration applying (Adaptive filtration system) AFS-009 and AFS-020 installations. The content of live microbial cells was determined by cytorefractometry. Assessment of the resistance of Y. pestis EV strain cells to technological factors was performed by photometric registration of changes in the optical density of bacterial suspension during the lytic response of cells to sodium dodecyl sulfate. Physical-chemical and immunobiological properties of the dry live plague vaccine were determined in accordance with the pharmacopoeial item.3.3.1.0021.15 of the State Pharmacopoeia of the Russian Federation, 14th edition.Results and discussion. The design features of the equipment introduced made it possible to carry out membrane filtration of microbial suspension, using BIOR-0.25 reactor as an intermediate storage unit, thereby excluding three technological stages. The total concentration of microbes in the suspension obtained by routine and improved methods was more than 150 billion microbial cells per ml. A comparative study of the effect of various hydrodynamic regimes in the working cavities of AFS-009 and AFS-020 filter units did not significantly affect the morphometric properties and resistance of microbial cultures to extreme (technological) factors. Based on the experimental data, the mass balance of the membrane filtration process has been determined. The optimized technology gave 0.13 liter yield of concentrate from 1 liter of native culture, and the process duration was reduced to 5 hours, the yield of the finished product in one production cycle was increased by 3 times. Thus, the process of concentrating Y. pestis EV strain cells during the production of the tablet form of live plague vaccine has been enhanced. A comparative study of the morphometric properties and resistance of plague microbe cultures to technological factors in the process of their concentration using optimized technology did not reveal any significant differences as compared to the routine one. Technological stage of concentrating has been reduced to 5 h term with a three-fold increase in the yield of finished product.
Lenticular-bedding-like bioturbation and the onshore recognition of carbonate drifts (Oligocene, Cyprus)
Authors: Reolid Jesús , Betzler Christian , Bialik Or M. , Waldman Nicolas 
Published: February 2021

ABSTRACTThe fine-grained carbonate deposits of the Oligocene to early Miocene of Cyprus are the most characteristic example of onshore outcropping carbonate drifts. These deposits were analyzed from a sedimentological and ichnological perspective, in order to determine the origin of the lenticular bedding characteristic of such deposits. The facies at the two study sections, Tsada and Petra Tou Romiou, consist of an alternation of thin, poorly cemented, intensely bioturbated marly limestone intervals and thick cemented wackestone intervals with abundant bioturbation and “lenticular bedding.” The ichnoassemblage, comprising Zoophycos, Thalassinoides, and Planolites is attributable to the Zoophycos Ichnofacies. The alternation of intervals with medium to well-preserved traces with completely biogenically homogenized facies reflects changes in substrate consistency related to changes in bottom-current velocity or to sedimentation. The presence of lenticular-bedding-like structures in the study deposits, traditionally considered diagnostic of bottom-current action in carbonate-drift outcrops, is shown to be exclusively the result of bioturbation. In the study sections, the “lenticular bedding” is the result of the coalescence of successive Zoophycos structures, which are readily preserved because they are constructed in the historical layer. It is proposed that the overlap of abundant isolated cone-shaped burrows of Zoophycos is the origin of the putative lenticular bedding recorded in the Oligocene fine-grained carbonate deposits of Cyprus, traditionally identified as drifts. Since this bedding is not related to currents in the study sections, the consideration of these carbonate deposits of Cyprus as drifts should be reevaluated. This has crucial implications for the recognition of carbonate drift outcrops elsewhere. Onshore carbonate drift outcrops wherein lenticular bedding is the main diagnostic criterion should be revisited and evaluated ichnologically.
FOSSILIZATION OF THE EOCENE “MONKEYHAIR” LATICIFER TREE FROM GEISELTAL, GERMANY: A DEEPER UNDERSTANDING USING MICRO-CT AND PYROLYSIS GC/MS
Authors: MCCOY VICTORIA E. , BOOM ARNOUD , WINGS OLIVER , WAPPLER TORSTEN , LABANDEIRA CONRAD C. , GEE CAROLE T. 
Published: February 2021

ABSTRACTAlthough the fibrous plant material called Affenhaar from the middle Eocene lignites of Geiseltal in Germany has been repeatedly studied for over 172 years, modern imaging and chemical analyses have enabled a deeper understanding of its chemical composition and preservation within the parent plant. Known in English as “monkeyhair,” the fibrous material represents a very rare case of fossil laticifers, the latex-bearing ducts of plants. Here we use high-resolution X-ray microcomputed tomography (micro-CT) for the first time to elucidate the anatomy of tissues and their differential degradation within the monkeyhair tree. Even in large, fairly intact stem axes, the center cylinder of secondary xylem inside the trunks or branches is completely absent, presumably due to diagenetic degradation. Nevertheless, there is a moderately well-preserved outer layer of bark and mats of excellently preserved laticifers in the extraxylary zone beneath the bark. Previous chemical analyses revealed that the latex in these laticifers was dominated by natural rubber (cis-1,4-polyisoprene), but also included various triterpenoids. These fossil laticifers also contained large amounts of organic sulfur, suggesting the rubber may have been naturally vulcanized. Pyrolysis GC/MS is also applied to the fossil laticifers for the first time to identify the organic sulfur constituents of this ancient latex. The chemical analysis yields 12 major sulfur compounds, mostly thiophenes, consistent with vulcanized rubber. The vulcanization of these laticifers most likely occurred during early diagenesis, before the degradation of the wood, at low temperatures, and may have been facilitated with other compounds that served as accelerators, activators, and fillers.
Clinical characteristics and prognosis of castleman disease patients in a Chinese hospital: paraneoplastic pemphigus is an independent risk factor
Authors: Hua Yibo , Liang Chao , Yang Jie , Wang Luyang , Xi Lei , Xu Aimin , Wang Shangqian , Wang Zengjun 
Published: February 2021

Abstract
Background: Castleman disease (CD) is a rare lymphoproliferative disorder that has had limited clinical research. This study aims to detect the clinical manifestations, pathological features, and prognostic factors of this disease.Methods: This study retrospectively analyzed the information of 54 patients with CD hospitalized in a single centre. A Cox regression model was employed to perform univariate analysis and multivariate analysis in order to identify independent prognostic factors for survival.Results: Based on clinical classification, 30 patients (55.6%) had unicentric CD (UCD) and 24 patients (44.4%) had multicentric CD (MCD). Moreover, pathological classification identified 32 cases (59.3%) with hyaline vascular variant (HV), 3 (5.6%) with mixed cellular variant (Mix), and 19 (35.2%) with plasmacytic variant (PC). The MCD patients commonly exhibited clinical signs and symptoms, including fever, splenomegaly, and pleural effusion and/or ascites. Several clinical complications, such as liver injury, anemia, and polyradiculoneuropathy, organomegaly, endocrinopathy, monoclonal plasma cell disorder, skin changes syndrome (POEMS) were more common in MCD patients. Univariate analysis showed that presence of paraneoplastic pemphigus (PNP) and elevated C-reactive protein (CRP) were unfavorable factors relating to CD patient survival. Multivariate analysis identified the presence of PNP as an independent prognostic factor in patients with CD.Conclusions: This study provided a panoramic elaboration of CD cases and showed the presence of PNP was an independent unfavorable factor.
Mismatch repair deficiency is associated with specific morphologic features and frequent loss of ARID1A expression in ovarian clear cell carcinoma
Authors: Ge Huijuan , Xiao Yaoxin , Qin Guangqi , Gu Yanzi , Cai Xu , Jiang Wenhua , Tu Xiaoyu , Yang Wentao , Bi Rui 
Published: February 2021

Abstract
Background Ovarian clear cell carcinoma (OCCC) is the second subtype of ovarian epithelial carcinoma reported to be closely related to Lynch syndrome (LS). ARID1A mutation is an important pathogenetic mechanism in OCCC that leads to loss of ARID1A expression in approximately half of OCCCs. However, the correlation of MMR status and ARID1A deficiency is unclear. The current study aimed to identify the clinical and histopathological characteristics of OCCC associated with dMMR and to further explore the association between dMMR and ARID1A deficiency. Methods A cohort of 176 primary OCCC patients was enrolled and review included histological characteristics (nuclear atypia, necrosis, mitosis, stromal hyalinization, and background precursors) and host inflammatory response (tumor-infiltrating lymphocytes, peritumoral lymphocytes, intratumoral stromal inflammation and plasma cell infiltration). Immunohistochemical staining of MLH1, PMS2, MSH2, MSH6 and ARID1A was performed using tissue microarrays.Results dMMR was detected in 10/176 tumors (6%), followed by MSH2/MSH6 (6/176), MLH1/PMS2 (3/176), and MSH6 (1/176). The average age of patients with dMMR was younger than that of patients with intact MMR (46 y vs 53 y). Tumors with diffuse intratumoral stromal inflammation remained significantly associated after multivariate analysis. ARID1A expression was absent in 8 patients with dMMR (8/10), which is a significantly higher frequency than that observed in patients with intact MMR (80% vs. 43.2%). Conclusion Our study indicates that diffuse intratumoral stromal inflammation of OCCCs is associated with dMMR, with loss of MSH2/MSH6 expression being most frequent. dMMR is strongly associated with the loss of ARID1A expression in OCCC.
Factors Associated With Herbal Medicine Use in Pregnancy Among Postnatal Mothers in Mbarara Regional Referral Hospital in Western Uganda
Authors: Muteebwa Laban , Ssetaala Ali , Muramuzi Dan , Nanvubya Annet , Mayanja Yunia 
Published: February 2021

Abstract
BackgroundThere is widespread use of herbal medicines among populations in sub-Saharan Africa. However, pregnant women should be conscious about medication taken during pregnancy including herbal medicines because their safety profiles are not known and some of them might affect the mother, fetus and pregnancy outcomes. Knowledge about use and safety of herbs in pregnant women is limited. This study aimed to assess the extent of use and the factors associated with use of herbal medicine in pregnancy.MethodsA cross-sectional study involving 385 women 6 weeks post-partum and below, receiving post-natal services at Mbarara Regional Referral Hospital in Western Uganda was conducted during May to August 2016. Simple random sampling was used to select participants. A structured pretested questionnaire was administered after written informed consent. Data was analyzed using logistic regression (STATA- 14).ResultsOf 385 respondents, 70.4% reported to have used herbal medicine during their most recent pregnancy. Associated factors were perception that herbal medicines are safe (aOR 9.8, 95% CI (4.2-23.0), perception that herbal medicines are important (aOR 12.4, 95% CI (5.2-29.5), staying more than 10KM from the heath facility (aOR 3.1, 95%CI (1.4-6.9), being a first time mother (aOR 2.6, 95%CI (1.1-6.2) and dissatisfaction with ANC services at health facility (aOR 2.6, 95%CI (1.1-6.3)ConclusionHerbal medicine use in pregnancy is common in the study area. Community Sensitization drives about the dangers of herbal medicine use in pregnancy is recommended. Healthcare workers should routinely screen for herbal medicine use during antenatal care visits and labor.
Multiplex Sequencing of SARS-Cov-2 genome directly from clinical samples using the Ion Personal Genome Machine (PGM)
Authors: Tan Kim-Kee , Tiong VunJia , Jia-Yi Tan , Wong Jo-Ern , Teoh Boon-Teong , Abd-Jamil Juraina , Johari Jefree , Nor'E Siti Sarah , Khor Chee-Sieng , Yaacob Che-Norainon , Zulkifli Mulya-Mustika-Sari , CheMatSeri AsmaAnati , Mahfodz Nur-Hidayana , Azizan Noor Syahida , AbuBakar Sazaly 
Published: February 2021

Abstract
Various methods have been developed for rapid and high throughput full genome sequencing of SARS-CoV-2. Here, we described a protocol for targeted multiplex full genome sequencing of SARS-CoV-2 genomic RNA directly extracted from human nasopharyngeal swabs using the Ion Personal Genome Machine (PGM). This protocol involves concomitant amplification of 237 gene fragments encompassing the SARS-CoV-2 genome to increase the abundance and yield of viral specific sequencing reads. Five complete and one near-complete genome sequences of SARS-CoV-2 were generated with a single Ion PGM sequencing run. The sequence coverage analysis revealed two amplicons (positions 13751-13965 and 23941-24106), which consistently gave low sequencing read coverage in all isolates except 4Apr20-64-Hu. We analyzed the potential primer binding sites within these low covered regions and noted that the 4Apr20-64-Hu possess C at positions 13730 and 23929, whereas the other isolates possess T at these positions. The genetic variations observed suggest that the naturally occurring genome variations present in the actively circulating SARS-CoV-2 strains affected the performance of the target enrichment panel of the Ion AmpliSeq™ SARS‑CoV‑2 Research Panel. The possible impact of other genetic sequence variations warrants further investigation, and an improved version of the Ion AmpliSeq™ SARS‑CoV‑2 Research Panel, hence, should be considered.
Effects of Propolis Supplementation on The Severity of Disease In Irritable Bowel Syndrome Subjects: A Randomized, Double-Blind Clinical Trial
Authors: Miryan Mahsa , Alavinejad Pejman , Abbaspour Mohammadreza , Soleimani Davood , Ostadrahimi Alireza 
Published: February 2021

Abstract
The effects of propolis, a well-known functional food, on irritable bowel syndrome (IBS), a chronic gastrointestinal disorder, in humans have yet to be investigated. This study evaluated propolis effects in IBS subjects. In this clinical study, 56 patients with IBS diagnosed by Rome IV criteria were assigned for 6 weeks randomly to the study groups. At the baseline and endpoint phase, patients’ gastrointestinal symptoms, quality of life (QOL), anxiety state, dietary intakes, and anthropometric indices were assessed. Independent t-test, paired t-test, Mann-Whitney U test, Wilcoxon, Fisher's exact test, repeated measures analysis of variance and logistic regression test were used for analyzing the data. To adjust the effect of confounders, covariance analysis was used. The results of this study showed that after modulating the effect of potential confounders, propolis supplementation increased the chance of improving IBS severity by 6.22 (with a confidence interval of: 1.33 - 1.14 and P = 0.035). A significant abdominal pain improvement, anxiety state, and bowel habits dissatisfaction reduction was observed within- and between-group differences in propolis group compared to the placebo group (P = 0.040, P = 0.035, P = 0.029, retrospectively). The overall score of quality of life and its domains in the propolis group was statistically significant, but in comparison between the two groups, this difference was not significant. Also, regards to the food intakes and anthropometric indices, there were no significant differences between and within the two study groups. This study illustrated that propolis supplementation could be used as adjunctive therapy in IBS disease to reduce abdominal pain and anxiety state.
Classification and Registration of Persistent Somatic Symptoms in Primary Care: A General Practitioner’s Perspective
Authors: Kitselaar Willeke , Vaart Rosalie van der , Boeft Madelon van Tilborg-den , Vos Hedwig , Numans Mattijs , Evers Andrea 
Published: February 2021

Abstract
Background: Persistent somatic symptoms (PSS) are common in primary care and often accompanied by an increasing disease burden for both the patient and healthcare. In medical practice, PSS is historically considered a diagnosis by exclusion or primarily seen as psychological. As a result, classification and registration of PSS in electronic health records (EHR) is hampered. The present study explores how general practitioners (GPs) currently register PSS, and what their view is regarding necessary improvements in classification, registration, and consultations.Method: Dutch GPs were invited by email to participate in a national cross-sectional online survey. The survey addressed ICPC-codes used by GPs to register PSS, PSS-related terminology added to free text areas, usage of PSS-related syndrome codes, and GPs’ need for improvement of PSS classification, registration and care.Results: GPs (n=259) were most likely to use codes specific to the symptom presented (89.3%). PSS-related terminology in free-text areas was used sparsely. PSS-related syndrome codes were reportedly used by 91.5% of GPs, but this was primarily the case for the code for irritable bowel syndrome. The ambiguous registration of PSS is reported as problematic by 47.9% of GPs. Over 56.7% of GPs reported needing additional schooling, tools or other support for PSS classification and consultation. GPs also reported needing other referral options and better guidelines.Conclusions: Registration of PSS in primary care is currently ambiguous. Approximately half of GPs felt a need for more options for registration of PSS and reported a need for further support. In order to improve classification, registration and care for patients with PSS, there is a need for a more appropriate coding scheme and additional schooling.