bluuebunny/crossref_metadata_2025_split huggingface dataset:
You Won’t Believe Our Results! But They Might: Heterogeneity in Beliefs About the Accuracy of Online Media
Authors: Luca Mario , Munger Kevin , Nagler Jonathan , Tucker Joshua A. 
Published: January 2021

Abstract“Clickbait” media has long been espoused as an unfortunate consequence of the rise of digital journalism. But little is known about why readers choose to read clickbait stories. Is it merely curiosity, or might voters think such stories are more likely to provide useful information? We conduct a survey experiment in Italy, where a major political party enthusiastically embraced the esthetics of new media and encouraged their supporters to distrust legacy outlets in favor of online news. We offer respondents a monetary incentive for correct answers to manipulate the relative salience of the motivation for accurate information. This incentive increases differences in the preference for clickbait; older and less educated subjects become even more likely to opt to read a story with a clickbait headline when the incentive to produce a factually correct answer is higher. Our model suggests that a politically relevant subset of the population prefers Clickbait Media because they trust it more.
New Precise Dating of the India‐Asia Collision in the Tibetan Himalaya at 61 Ma
Authors: An Wei , Hu Xiumian , Garzanti Eduardo , Wang Jian‐Gang , Liu Qun 
Published: January 2021

AbstractThe timing of the India‐Asia collision onset, essential to understanding the evolution of the Himalayan‐Tibetan orogen, has been widely investigated through multidisciplinary approaches. Among these, the India to Asia provenance reversal (IAPR) documented in the Indian passive margin successions has proved to be most effective. We present integrated stratigraphic, sedimentological, and provenance data on Upper Cretaceous‐Paleogene strata from the newly investigated Mubala section exposed south of the Yarlung‐Zangbo suture zone (YZSZ) in southern Tibet, which preserves continuous deep‐marine turbiditic and biogenic sedimentation on the distal Indian passive margin. Sandstone petrography, heavy minerals, detrital zircon geochronology and Hf isotopes, and detrital Cr‐spinel geochemistry constrain the IAPR to later than 62.7 Ma (youngest zircon ages from the earliest Asian‐derived sandstone) and by 61.0 ± 0.3 Ma (SIMS age of a tuffaceous layer ∼30 m above this bed). The onset of intercontinental collision along the YZSZ began by 61 Ma.
Wave‐Driven Flow Along a Compact Marginal Ice Zone
Authors: Thomson Jim , Lund Björn , Hargrove John , Smith Madison M. , Horstmann Jochen , MacKinnon Jennifer A. 
Published: January 2021

AbstractObservations of surface waves and ice drift along a compact sea ice edge demonstrate the importance of waves in a marginal ice zone. An analytic model is presented for the along‐ice drift forced by the radiation stress gradient of oblique waves. A momentum balance using quadratic drag to oppose the wave forcing is sufficient to explain the observations. Lateral shear stresses in the ice are also evaluated, though this balance does not match the observations as well. Additional forcing by local winds is included and is small relative to the wave forcing. However, the wave forcing is isolated to a narrow region around 500‐m wide, whereas the wind forcing has effects on larger scales. The simplistic drag is assessed using observations of shear and turbulent dissipation rates. The results have implications for the shape and evolution of the ice edge, because the lateral shear may be a source of instabilities.
Contrasting Effect of Soil Moisture on the Daytime Boundary Layer Under Different Thermodynamic Conditions in Summer Over China
Authors: Xu Zhiqi , Chen Haishan , Guo Jianping , Zhang Wanchun 
Published: January 2021

AbstractThe investigation is still lacking concerning the effect of soil moisture (SM) on the evolution of planetary boundary layer (PBL) under different land conditions in a huge domain as large as China. We perform an explicit correlation analysis between daytime PBL height (PBLH) and SM for convective, neutral and stable boundary layer regimes (i.e., CBL, NBL, and SBL), respectively. A negative correlation exists between SM and daytime PBLH for CBL and NBL, exhibiting a spatial pattern of “east strong west weak”, albeit a positive correlation for SBL. The standard deviation of PBLH for CBL and NBL exhibits a spatial pattern of “northwest high southeast low”. Cloudy, humid, and stable atmosphere result in CBL shoaling. PBLH more correlates with the sensible heat flux (CBL: r = 0.25; NBL: r = 0.33) over dry areas, but over Northwest China the PBL depends more on meteorology likely owing to the extremely dry soil.
Tree‐Ring Reconstruction of the Atmospheric Ridging Feature That Causes Flash Drought in the Central United States Since 1500
Authors: Bolles Kasey C. , Williams A. Park , Cook Edward R. , Cook Benjamin I. , Bishop Daniel A. 
Published: January 2021

AbstractRapid drought intensification, or flash droughts, is often driven by anomalous atmospheric ridging and can cause severe and complex impacts on water availability and agriculture, but the full range of variability of such events in terms of intensity and frequency is unknown. New tree‐ring reconstructions of May–July mid‐tropospheric ridging and soil moisture anomalies back to 1500 CE in the central United States—a hotspot for flash drought—suggest that over the last five centuries, anomalies in these two variables combined to indicate flash‐drought conditions in ∼17% of years and exceptionally severe flash drought in ∼4% of years, similar to frequencies in recent decades. However, over one‐third of all inferred exceptional flash droughts occurred since 1900, suggesting the 20th century was highly flash‐drought prone. These results may guide future work to diagnose the roles of external, oceanic, and land‐surface forcing of warm‐season atmospheric circulation and hydroclimate over North America.
Whistler and Broadband Electrostatic Waves in the Multiple X‐Line Reconnection at the Magnetopause
Authors: Zhong Z. H. , Graham D. B. , Khotyaintsev Yu. V. , Zhou M. , Le Contel O. , Tang R. X. , Deng X. H. 
Published: January 2021

AbstractWe investigate whistler‐mode waves and broadband electrostatic waves (EWs) within an ion diffusion region (IDR) at the magnetopause. The quasi‐parallel whistlers are observed in the separatrix regions associated with the electron anisotropy or loss cone, while the oblique whistlers, the Buneman‐type waves, and the oblique EWs are observed in the center of the current sheet associated with the accelerated electron or ion beams. The whistlers are linked with Buneman‐type waves by the electron Pacman distribution rather than the wave‐wave process. The accelerated cold electron beams excite the Buneman‐type instabilities and make the anisotropy or loss cone of hot electrons less apparent, which led to the conversion of the whistlers from quasi‐parallel to oblique. Additionally, the oblique EWs are associated with the ion beams produced by the multiple X‐line reconnection. These results provide a further understanding of the relation between plasma waves and plasma kinetics in the IDR.
European Windstorm Risk of Post‐Tropical Cyclones and the Impact of Climate Change
Authors: Haarsma Rein 
Published: January 2021

AbstractSainsbury et al. (2020, https://doi.org/10.1029/2020GL089853) highlighted the fact that although post‐tropical cyclones only consist of a small part of the cyclones impacting Northern Europe (∼1%) during the hurricane season, their contribution to storms with at least storm force (>25 m/s) is about 10 times larger and therefore pose a substantial windstorm risk for Northern Europe. Overall post‐TCs impacting Northern Europe are significantly more intense than midlatitude cyclones. This is related to their combined tropical (warm seawater) and midlatitude (baroclinic instability) energy source. A few studies have suggested that in a warmer climate the amount and intensity of post‐tropical cyclones might increase due to stronger tropical cyclones (TCs), warmer oceans, and pole and eastward extension of TC genesis regions. This would further enhance the windstorm risk of post‐TCs. Further high‐resolution modeling research is required to delineate this risk.
New Constraints on Slip Deficit on the Aleutian Megathrust and Inflation at Mt. Veniaminof, Alaska From Repeat GPS Measurements
Authors: Drooff Connor , Freymueller Jeffrey T. 
Published: January 2021

AbstractWe employ an enhanced data set of Global Positioning System (GPS) velocities to reassess the slip deficit along the plate interface of the Alaska‐Aleutian subduction zone. An examination of velocities of sites near Mt Veniaminof, located in the southwest Alaska Peninsula, shows that existing models for tectonic deformation do not accurately predict the local velocity field along this section of the peninsula. In a combined model, we solve for the volcanic inflation signal on the edifice of Mt. Veniaminof and reassess the variations in trench coupling outboard of the volcano. The interseismic deformation near Veniaminof and the eastern Shumagin islands requires an additional model segment with higher slip deficit than the western Shumagin islands. This segment appears to have been the main rupture patch of the 2020 M 7.9 Simeonof earthquake. The volcano inflated over the time period of 2005–2017, although at a lower rate than in 2002–2005.
Multiscale Legacy Responses of Soil Gas Concentrations to Soil Moisture and Temperature Fluctuations
Authors: Parolari Anthony J. , Sizemore Joseph , Katul Gabriel G. 
Published: January 2021

AbstractThe sensitivity of soil carbon dynamics to climate change is a major uncertainty in carbon cycle models. Of particular interest is the response of soil biogeochemical cycles to variability in hydroclimatic states and the related quantification of soil memory. Toward this goal, the power spectra of soil hydrologic and biogeochemical states were analyzed using measurements of soil temperature, moisture, oxygen, and carbon dioxide at two sites. Power spectra indicated multiscale power law scaling across subhourly to annual timescales. Precipitation fluctuations were most strongly expressed in the soil biogeochemical signals at monthly to annual timescales. Soil moisture and temperature fluctuations were comparable in strength at one site, while temperature was dominant at the other. The effect of soil hydrologic, thermal, and biogeochemical processes on gas concentration variability was evidenced by low spectral entropy relative to the white noise character of precipitation. A full mass balance model was unable to capture high‐frequency soil temperature influence, indicating a gap in commonly used model assumptions. A linearized model was shown to capture the main features of the observed and modeled gas concentration spectra and demonstrated how the means and variances of soil moisture and temperature interact to produce the gas concentration spectra. Breakpoints in the spectra corresponded to the mean rate of gas efflux, providing a first‐order estimate of the soil biogeochemical integral timescale (∼1 min). These methods can be used to identify biogeochemical system dynamics to develop robust, process‐based soil biogeochemistry models that capture variability in addition to long‐term mean values.
The Influence of Anisotropic Sediment Layer on Dissolved Oxygen Transfer in Turbulent Flows
Authors: Wang Kunpeng , Li Qingxiang , Zhou Quan , Dong Yuhong 
Published: January 2021

AbstractThe concentration and distribution of dissolved oxygen (DO) are critical to the self‐purification capacity of water and the survival of aquatic organisms, and they are important factors considered in the ecological balance system of water resources. In this work, the high‐Schmidt number DO transfer phenomenon in open channel flows with anisotropic sediment beds is studied using large eddy simulation. The volume‐averaged Navier‐Stokes equations coupled with the Monod equation is used to describe the in‐sediment flow and the sediment oxygen demand of micro‐organisms, so as to examine the transport characteristics of DO inside and outside the sediment layer. The effects of various permeable conditions of sediments (characterized by the anisotropy index of permeability, i.e., λ) on the distribution of DO concentration and flux, penetration depth, and mass transfer processes is studied by means of the time‐averaged statistics variations in flow and scalar fields. A larger anisotropy index λ is found can enhance the transport of DO from the water flowing into the sediment layer, which is caused by turbulent diffusions and turbulence bursting events. It suggests that the λ‐dependent distributions of the DO fluctuations and fluxes are highly correlated with the coherent structures in the turbulence over the sediment‐water interface (SWI). A key relationship between the Sherwood number and the anisotropy index is found by means of the current simulations and also is verified by our theoretical analysis. The dependence of λ on DO penetration depth is consistent with the hypothesis that coherent turbulence in the water column drives mass transport across the SWI.
The Met1-linked ubiquitin machinery in inflammation and infection
Authors: Fiil Berthe Katrine , Gyrd-Hansen Mads 
Published: January 2021

AbstractUbiquitination is an essential post-translational modification that regulates most cellular processes. The assembly of ubiquitin into polymeric chains by E3 ubiquitin ligases underlies the pleiotropic functions ubiquitin chains regulate. Ubiquitin chains assembled via the N-terminal methionine, termed Met1-linked ubiquitin chains or linear ubiquitin chains, have emerged as essential signalling scaffolds that regulate pro-inflammatory responses, anti-viral interferon responses, cell death and xenophagy of bacterial pathogens downstream of innate immune receptors. Met1-linked ubiquitin chains are exclusively assembled by the linear ubiquitin chain assembly complex, LUBAC, and are disassembled by the deubiquitinases OTULIN and CYLD. Genetic defects that perturb the regulation of Met1-linked ubiquitin chains causes severe immune-related disorders, illustrating their potent signalling capacity. Here, we review the current knowledge about the cellular machinery that conjugates, recognises, and disassembles Met1-linked ubiquitin chains, and discuss the function of this unique posttranslational modification in regulating inflammation, cell death and immunity to pathogens.
Structure of a microtubule-bound axonemal dynein
Authors: Walton Travis , Wu Hao , Brown Alan 
Published: January 2021

AbstractAxonemal dyneins are tethered to doublet microtubules inside cilia to drive ciliary beating, a process critical for cellular motility and extracellular fluid flow. Axonemal dyneins are evolutionarily and biochemically distinct from cytoplasmic dyneins that transport cargo, and the mechanisms regulating their localization and function are poorly understood. Here, we report a single-particle cryo-EM reconstruction of a three-headed axonemal dynein natively bound to doublet microtubules isolated from cilia. The slanted conformation of the axonemal dynein causes interaction of its motor domains with the neighboring dynein complex. Our structure shows how a heterotrimeric docking complex specifically localizes the linear array of axonemal dyneins to the doublet microtubule by directly interacting with the heavy chains. Our structural analysis establishes the arrangement of conserved heavy, intermediate and light chain subunits, and provides a framework to understand the roles of individual subunits and the interactions between dyneins during ciliary waveform generation.
Distinct mechanisms control genome recognition by p53 at its target genes linked to different cell fates
Authors: Farkas Marina , Hashimoto Hideharu , Bi Yingtao , Davuluri Ramana V. , Resnick-Silverman Lois , Manfredi James J. , Debler Erik W. , McMahon Steven B. 
Published: January 2021

AbstractThe tumor suppressor p53 integrates stress response pathways by selectively engaging one of several potential transcriptomes, thereby triggering cell fate decisions (e.g., cell cycle arrest, apoptosis). Foundational to this process is the binding of tetrameric p53 to 20-bp response elements (REs) in the genome (RRRCWWGYYYN0-13RRRCWWGYYY). In general, REs at cell cycle arrest targets (e.g. p21) are of higher affinity than those at apoptosis targets (e.g., BAX). However, the RE sequence code underlying selectivity remains undeciphered. Here, we identify molecular mechanisms mediating p53 binding to high- and low-affinity REs by showing that key determinants of the code are embedded in the DNA shape. We further demonstrate that differences in minor/major groove widths, encoded by G/C or A/T bp content at positions 3, 8, 13, and 18 in the RE, determine distinct p53 DNA-binding modes by inducing different Arg248 and Lys120 conformations and interactions. The predictive capacity of this code was confirmed in vivo using genome editing at the BAX RE to interconvert the DNA-binding modes, transcription pattern, and cell fate outcome.
Molecular determinants and mechanism for antibody cocktail preventing SARS-CoV-2 escape
Authors: Ku Zhiqiang , Xie Xuping , Davidson Edgar , Ye Xiaohua , Su Hang , Menachery Vineet D. , Li Yize , Yuan Zihao , Zhang Xianwen , Muruato Antonio E. , i Escuer Ariadna Grinyo , Tyrell Breanna , Doolan Kyle , Doranz Benjamin J. , Wrapp Daniel , Bates Paul F. , McLellan Jason S. , Weiss Susan R. , Zhang Ningyan , Shi Pei-Yong , An Zhiqiang 
Published: January 2021

AbstractAntibody cocktails represent a promising approach to prevent SARS-CoV-2 escape. The determinants for selecting antibody combinations and the mechanism that antibody cocktails prevent viral escape remain unclear. We compared the critical residues in the receptor-binding domain (RBD) used by multiple neutralizing antibodies and cocktails and identified a combination of two antibodies CoV2-06 and CoV2-14 for preventing viral escape. The two antibodies simultaneously bind to non-overlapping epitopes and independently compete for receptor binding. SARS-CoV-2 rapidly escapes from individual antibodies by generating resistant mutations in vitro, but it doesn’t escape from the cocktail due to stronger mutational constraints on RBD-ACE2 interaction and RBD protein folding requirements. We also identified a conserved neutralizing epitope shared between SARS-CoV-2 and SARS-CoV for antibody CoV2-12. Treatments with CoV2-06 and CoV2-14 individually and in combination confer protection in mice. These findings provide insights for rational selection and mechanistic understanding of antibody cocktails as candidates for treating COVID-19.
High-fidelity DNA ligation enforces accurate Okazaki fragment maturation during DNA replication
Authors: Williams Jessica S. , Tumbale Percy P. , Arana Mercedes E. , Rana Julian A. , Williams R. Scott , Kunkel Thomas A. 
Published: January 2021

AbstractDNA ligase 1 (LIG1, Cdc9 in yeast) finalizes eukaryotic nuclear DNA replication by sealing Okazaki fragments using DNA end-joining reactions that strongly discriminate against incorrectly paired DNA substrates. Whether intrinsic ligation fidelity contributes to the accuracy of replication of the nuclear genome is unknown. Here, we show that an engineered low-fidelity LIG1Cdc9 variant confers a novel mutator phenotype in yeast typified by the accumulation of single base insertion mutations in homonucleotide runs. The rate at which these additions are generated increases upon concomitant inactivation of DNA mismatch repair, or by inactivation of the Fen1Rad27 Okazaki fragment maturation (OFM) nuclease. Biochemical and structural data establish that LIG1Cdc9 normally avoids erroneous ligation of DNA polymerase slippage products, and this protection is compromised by mutation of a LIG1Cdc9 high-fidelity metal binding site. Collectively, our data indicate that high-fidelity DNA ligation is required to prevent insertion mutations, and that this may be particularly critical following strand displacement synthesis during the completion of OFM.
STIM-Orai1 signaling regulates fluidity of cytoplasm during membrane blebbing
Authors: Aoki Kana , Harada Shota , Kawaji Keita , Matsuzawa Kenji , Uchida Seiichi , Ikenouchi Junichi 
Published: January 2021

AbstractThe cytoplasm in mammalian cells is considered homogeneous. In this study, we report that the cytoplasmic fluidity is regulated in the blebbing cells; the cytoplasm of rapidly expanding membrane blebs is more disordered than the cytoplasm of retracting blebs. The increase of cytoplasmic fluidity in the expanding bleb is caused by a sharp rise in the calcium concentration. The STIM-Orai1 pathway regulates this rapid and restricted increase of calcium in the expanding blebs. Conversely, activated ERM protein binds to Orai1 to inhibit the store-operated calcium entry in retracting blebs, which results in decreased in cytoplasmic calcium, rapid reassembly of the actin cortex.
Fast micro-computed tomography data of solute transport in porous media with different heterogeneity levels
Authors: Van Offenwert Stefanie , Cnudde Veerle , Boone Marijn , Bultreys Tom 
Published: January 2021

AbstractSolute transport processes are influenced by pore-scale heterogeneity. To study this, transient micron-scale solute concentration fields were imaged by fast laboratory-based X-ray micro-computed tomography. We performed tracer injection experiments in three types of porous material with increasing levels of heterogeneity (sintered glass, Bentheimer sandstone and Savonnières limestone). Different Peclet numbers were used during the experiments. For each sample and Peclet number, datasets of 40 to 74 3D images were acquired by continuous scanning with a voxel size of 13.4 to 14.6 µm and a temporal resolution of 15 to 12 seconds. To determine the measurement uncertainty on the obtained concentration fields, we performed calibration experiments under similar circumstances (temporal resolution of 12 seconds and voxel size of 13.0 µm). Here, we provide a systematic description of the data acquisition and processing and make all data, a total of 464 tomograms, publicly available. The combined dataset offers new opportunities to study the influence of pore-scale heterogeneity on solute transport, and to validate pore-scale simulations of this process in increasingly complex samples.
Antibacterial apple cider vinegar eradicates methicillin resistant Staphylococcus aureus and resistant Escherichia coli
Authors: Yagnik Darshna , Ward Malcolm , Shah Ajit J. 
Published: January 2021

AbstractMethicillin-resistant Staphylococcus aureus (MRSA) and resistant Escherichia coli (rE.coli) infections can spread rapidly. Further they are associated with high morbidity and mortality from treatment failure. Therapy involves multiple rounds of ineffective antibiotics alongside unwanted side effects, alternative treatments are crucial. Apple cider vinegar (ACV) is a natural, vegan product that has been shown to have powerful antimicrobial activity hence we investigated whether ACV could ameliorate these resistant bacteria. The minimum dilution of ACV required for growth inhibition was comparable for both bacteria (1/25 dilution of ACV liquid and ACV tablets at 200 µg/ml were effective against rE. coli and MRSA). Monocyte co-culture with microbes alongside ACV resulted in an increase in monocyte phagocytosis by 21.2% and 33.5% compared to non-ACV treated but MRSA or rE. coli stimulated monocytes, respectively. Label free quantitative proteomic studies of microbial protein extracts demonstrated that ACV penetrated microbial cell membranes and organelles, altering the expression of key proteins. This resulted in significant reductions in total protein expression, moreover we could only detect ribosomal proteins; 50 s 30 s, enolase, phosphenol pyruvate and the ATP synthase subunit in rE. coli. Elongation factor iNOS and phosphoglycerate kinase OS were the only proteins present in MRSA samples following ACV treatment.