bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Retrospective Study on Sex Difference in Patients With Urolithiasis: Who Is More Vulnerable to Chronic Kidney Disease?
Authors: Li Ching-Chia , Chien Tsu-Ming , Lu Yen-Man , Chou Yii-Her 
Published: February 2021

Abstract
Background: Urolithiasis is considered a vital public health issue with a substantial burden on kidney function. Additionally, only few reports focused on the gender difference in patients with urolithiasis. Therefore, this study aimed to compare the clinical characteristics of sex difference and their potential risk for chronic kidney disease (CKD) in patients with urolithiasis.Methods: Patients diagnosed with stone disease from 2013 to 2018 were retrospectively reviewed and divided into two groups by gender. Clinical demographic characteristics, stone location, stone composition, urine chemistries, and renal function were investigated. Univariate and multivariate analyses were used to assess the relationship and potential risk of CKD between sex groups.Results: A total of 1,802 stones were included: 1,312 from men and 490 from women. Female patients had higher rate of hypertension, diabetes, and dyslipidemia. Male patients predominantly had calcium-containing stones, especially calcium oxalate stone, uric acid stone, and struvite stone. Carbonate apatite stone was more frequently found in women. Complex surgeries such as percutaneous nephrolithotomy (PCNL) and ureteroscopic lithotripsy (URSL) were more frequently performed in women than that in men. Multivariate analysis confirmed that age >60 years (odds ratios [ORs] = 6.36; 95% confidence interval [CI], 3.8–10.8), female sex (ORs = 5.31; 95% CI 3.3–8.4), uric acid stone (ORs = 3.55; 95% CI 2.0–6.4), hypertension (OR = 7.20; 95% CI 3.8–13.7), and diabetes (OR = 7.06; 95% CI 3.1–16.2) were independent predictors of poor prognoses in CKD.Conclusions: Gender is significantly associated with higher prevalence of CKD among patients with urolithiasis. Therefore, women with stone disease may need close renal function monitoring during follow-up.
Decellularization of kidney tissue: Comparison of sodium lauryl ether sulfate and sodium dodecyl sulfate for allotransplantation in rat
Authors: Keshvari Mohammad Amin , Afshar Alireza , Daneshi Sajad , Khoradmehr Arezoo , Baghban Mandana , Muhaddesi Mahdi , Behrouzi Pouya , Miri Mohammad Reza , Azari Hossein , Nabipour Iraj , Shirazi Reza , Mahmudpour Mehdi , Tamadon Amin 
Published: February 2021

Abstract
Background: Chronic kidney diseases and end stage renal disease are growing threats worldwide. Tissue engineering is a new hope to surpass the current limitations such as the shortage of donor. To do so, the first step would be fabrication of an intact decellularized kidney scaffold. In the current study, an automatic decellularization device was developed to perfuse and decellularize male rats' kidneys using both sodium lauryl ether sulfate (SLES) and sodium dodecyl sulfate (SDS) and to compare their efficacy in kidney decellularization and post-transplantation angiogenesis.Methods: After anesthesia, kidneys were perfused with either 1% SDS solution for 4 h or 1% SLES solution for 6 h. The decellularized scaffolds were stained with hematoxylin and eosin, periodic acid Schiff, Masson’s trichrome, and Alcian blue to determine cell removal and glycogen, collagen and glycosaminoglycan contents, respectively. Moreover, scanning electron microscopy was performed to evaluate the cell removal and preservation of microarchitecture of both SDS and SLES scaffolds. Additionally, DNA quantification assay was applied for all groups in order to measure residual DNA in the scaffolds and normal kidney. In order to demonstrate biocompatibility and bioactivity of the decellularized scaffolds two tests were done. The scaffolds were recellularized with the human umbilical cord mesenchymal stromal/stem cells (hUC-MSCs). In addition, the allotransplantation was performed in back muscle and angiogenesis was evaluated.Results: Complete cell removal in both SLES and SDS groups was observed in scanning electron microscopy and DNA quantification assays. Moreover, the extracellular matrix architecture of rat kidney in the SLES group was significantly preserved better than the SDS group. The hUC-MSCs were successfully migrated from the cell culture plate surface into the SDS and SLES decellularized scaffolds. The formation of blood capillaries and vessels were observed in the kidney allotransplantation in both SLES and SDS decellularized kidneys.Conclusions: We demonstrated that both SLES and SDS could be promising tools in kidney tissue engineering. The better preservation of extracellular matrix than SDS, introduces SLES as the solvent of choice for kidney decellularization.
Design, synthesis and evaluation of benzimidazole-NsCOXis hybrids for the treatment of Alzheimer’s disease
Authors: Kaur Sukhvir , Minhas Richa , Kaur Birpal , bansal Yogita , Bansal Gulshan 
Published: February 2021

Abstract
Usage of an acetylcholinesterase (AChE) inhibitor and a non-selective COX inhibitor (NsCOXi) have been documented to exhibit significantly protective and recuperative effects in AD patients. Therefore, it is hypothesised that a compound capable of exhibiting both AChE inhibition and anti-inflammatory activities can be potential pluripotent drug candidate for de-accelerating the progression of AD, as well as for providing relief from the associated inflammatory pathological indications. The present study involves the coupling of ibuprofen (IB) or naproxen (NP) to varied disubstituted amines (AChE inhibitor pharmacophore) through benzimidazole to develop two series of compounds i.e. IB01-IB05 and NP01-NP05 as pluripotent anti-AD compounds. All target compounds are evaluated for in vitro AChE inhibitory and COX inhibitory activities. Compounds IB01-IB05 are found more potent as compared to NP01-NP05. Compound IB04 being the most active in in vitro evaluation is selected for in vivo evaluation of memory restoration using scopolamine-induced amnesia model in mice. It significantly reverses the scopolamine-induced changes (i.e., escape latency time, mean time spent in target quadrant, brain AChE activity and oxidative stress) in a dose-dependent manner. IB04 at higher dose i.e. 8 mg/kg is significantly effective in lowering AD manifestation in comparison to donepezil. The findings indicate that Benzimidazole-NsCOXi derivatives having pyrrolidine moiety may prove a useful template for the development of new chemical moieties against AD with multiple potencies.
A Cosmological Fireball with Thirty-Percent Gamma-Ray Radiative Efficiency
Authors: Li Liang , Wang Yu , Ryde Felix , Pe'er Asaf , Zhang Bing , Guiriec Sylvain , Castro-Tirado Alberto , Kann David Alexander , Axelsson Magnus , Page Kim , Veres Peter , Bhat Narayana Perumpalli 
Published: February 2021

Abstract
Gamma-ray bursts (GRBs) are the most powerful explosions in the universe. The composition of the jets is, however, subject to debate\cite{Peer2015,Zhang2018}. Whereas the traditional model invokes a relativistic matter-dominated fireball with a bright photosphere emission component\cite{Meszaros2000}, the lack of the detection of such a component in some GRBs\cite{Abdo2009} has led to the conclusion that GRB jets may be Poynting-flux-dominated\cite{Zhang2009}. Furthermore, how efficiently the jet converts its energy to radiation is poorly constrained. A definitive diagnosis of the GRB jet composition and measurement of GRB radiative efficiency requires high-quality prompt emission and afterglow data, which has not been possible with the sparse observations in the past. Here we report a comprehensive temporal and spectral analysis of the TeV-emitting bright GRB 190114C. Its fluence is one of the highest of all GRBs detected so far, which allows us to perform a high-significance study on the prompt emission spectral properties and their variations down to a very short timescale of about 0.1 s. We identify a clear thermal component during the first two prompt emission episodes, which is fully consistent with the prediction of the fireball photosphere model. The third episode of the prompt emission is consistent with synchrotron radiation from the deceleration of the fireball. This allows us to directly dissect the fireball energy budget in a parameter-independent manner\cite{Zhang2021} and robustly measure a nearly $30\%$ radiative efficiency for this GRB. The afterglow microphysics parameters can be also well constrained from the data. GRB 190114C, therefore, exhibits the evolution of a textbook-version relativistic fireball, suggesting that fireballs can indeed power at least some GRBs with high efficiency.
Genetic transformation technologies for the common dandelion, Taraxacum officinale
Authors: Dinkeloo Kasia , Cantero-Garcia Araceli Maria , Paik Inyup , Vulgamott Alexa , Ellington Andrew , Lloyd Alan 
Published: February 2021

Abstract
BackgroundTaraxacum officinale, or the common dandelion, is a widespread perennial species recognized worldwide as a common lawn and garden weed. Common dandelion is also cultivated for use in teas, as edible greens, and for use in traditional medicine. It produces latex and is closely related to the Russian dandelion, T. kok-saghyz, which is being developed as a rubber crop. Finally, the vast majority of extant common dandelions reproduce asexually through apomictically derived seeds-an important goal for many major crops in modern agriculture. There is increasing interest in the molecular control of important pathways as well as in the basic molecular biology and reproduction of common dandelion.ResultsHere we present an improved Agrobacterium-based genetic transformation and regeneration protocol, a protocol for generation and transformation of protoplasts using free DNA, and a protocol for leaf Agrobacterium infiltration for transient gene expression. These protocols use easily obtainable leaf explants from soil-grown plants and reagents common to most molecular plant laboratories. We show that common markers used in many plant transformation systems function as expected in common dandelion including fluorescent proteins, GUS, anthocyanin regulation, as well as resistance to kanamycin, basta, and hygromycin. ConclusionReproducible, stable and transient transformation methods are presented that will allow needed molecular structure and function studies of genes and proteins in T. officinale.
Preparation of β(1→3)/β(1→4)-xylooligosaccharides from red alga dulse by two xylanases from Streptomyces thermogriseus&nbsp;
Authors: Fujii Yuki , Kobayashi Manami , Miyabe Yoshikatsu , Kishimura Hideki , Hatanaka Tadashi , Kumagai Yuya 
Published: February 2021

Abstract
Red alga dulse has xylan with the structure containing β(1→3)/β(1→4)-linkage. We previously prepared xylooligosaccharides (XOS) from dulse xylan, however, the product contains many D-xylose and less XOS having β(1→3)-linkage. To improve the efficiency of XOS production, we prepared two recombinant endo-xylanases from Streptomyces thermogriseus (StXyl10 and StXyl11). Comparing the kcat/Km value for dulse xylan, the value of StXyl10 was approximately two times higher than that of StXyl11. We then determined the suitable condition for XOS production. As a result, the dulse XOS was prepared by the successive hydrolysis of 10 mg/ml of dulse xylan by 0.5 µg/ml StXyl10 for 4 h at 50°C and then 2.0 µg/ml StXyl11 for 36 h at 60°C. The xylan was converted into 95.8% of XOS, including 59.7% of XOS having β(1→3)-linkage, and 0.97% of X1. Our study provide useful information for the production of XOS having β(1→3)-linkage.
LncRNA HOXD-AS1 functions as a ceRNA to promote hepatocellular carcinoma metastasis in zebrafish xenograft models
Authors: Zhang Ying , Wang Xiaolu , Qin Feng , Jia Shaochang 
Published: February 2021

Abstract
Background: A few studies have shown that long noncoding RNA (lncRNA) HOXD cluster antisense RNA 1 (HOXD-AS1) plays an important role in hepatocellular carcinoma (HCC) metastasis as a competing endogenous RNA (ceRNA), but there is little in vivo evidence. This study aims to explore the zebrafish HCC xenograft as an in vivo metastasis model to verify the ceRNA network of HOXD-AS1. Methods: The quantitative reverse transcription PCR (qRT-PCR) assay was used to assess the expression level of HOXD-AS1 in HCC cell lines. Knockdown of HOXD-AS1 or miR-130a-3p was performed by transfecting small interfering RNA (siRNA) or microRNA (miRNA) inhibitor, respectively. The proliferation and invasion of HCC cells in vitro were analyzed by CCK-8 and transwell assays. The growth and metastasis of HCC cells in vivo were assessed by zebrafish xenograft models.Results: We verified that HOXD-AS1 was overexpressed in all tested HCC cell lines than the normal hepatic cells. Silence of HOXD-AS1 suppressed cell proliferation and invasion in Hep3B and Huh7 HCC cell lines in vitro. In zebrafish xenograft models, knockdown of HOXD-AS1 also reduced the growth and metastasis of the two HCC cells. Moreover, downregulation of miR-130a-3p not only increased the HCC metastasis, but also rescued the metastasis which inhibited by silence of HOXD-AS1 in vitro and in vivo.Conclusions: Our study demonstrates the metastasis role of the HOXD-AS1/miR-130a-3p ceRNA network in HCC cells in vitro and in vivo, and these findings suggest that zebrafish xenograft model could be used for ceRNA mechanism verification in tumor metastasis.
Anti-Tumoral Effects of A Benzimidazolyl Carbamate Ester Derivative on Head and Neck Squamous Carcinoma Cell Lines
Authors: Nicolai Alice , Madia Valentina Noemi , Messore Antonella , Leo Alessandro De , Ialongo Davide , Tudino Valeria , Tortorella Elisabetta , Scipione Luigi , Taurone Samanta , Pergolizzi Tiziano , Artico Marco , Santo Roberto Di , Costi Roberta , Scarpa Susanna 
Published: February 2021

Abstract
Nocodazole is a well-known anti-proliferative agent, thanks to its anti-microtubule activity. Herein we report a benzimidazolyl carbamate ester derivative, namely RDS 60, as structural analog of this compound. We evaluated the anti-neoplastic properties of RDS 60 in two human head and neck squamous cell carcinoma (HNSCC) cell lines. We found that RDS 60 significantly inhibited replication of both HNSCC without inducing any significant cytotoxic effect on non-transformed human dermal fibroblasts. The treatment of the two cell lines with 1 µM RDS 60 for 24 hours determined the incapacity of cells to develop normal bipolar mitotic spindles contemporaneously to a block of the cell cycle in G2/M and to cytoplasmic accumulation of cyclin B1. Moreover, doubling the dosage of RDS 60, an activation of apoptosis in both HNSCC was observed. Additionally, RDS 60 treatment reversed the epithelial-mesenchymal transition and inhibited cell migration and extracellular matrix infiltration of both HNSCC. These results demonstrate that this compound has a potent effect in blocking cell cycle, inducing apoptosis and inhibiting cell motility and invasion of the two HNSCC cell lines. Therefore, the ability of RDS 60 to attenuate the malignant phenotype of HNSCC suggests its potential role as an interesting and powerful tool for new approaches in treating these tumors.
Assessing the feasibility of a social worker-led non-communicable disease self-management intervention for low-income women elderly: a pilot trial
Authors: Wu Xueji , Chen Xiongfei , Pan Bingying , Liu Lan , Dong Xiaomei , Yu Wanwen , Parker David L. , Liao Bagen , Hu Min 
Published: February 2021

Abstract
Background Low-income Chinese women aged 60 years and older are more likely to have multiple non-communicable diseases (NCDs). Neither the independent healthcare system nor the social work system based on the communities is equipped to address their complex needs of health and daily care. The objective was to design and evaluate a social worker-led self-management program for older community-dwelling women with NCDs. MethodsA total of 144 and 138 subjects were recruited in the intervention and control groups from 10 communities in Guangzhou, China. The intervention group was designed as follows: each community recruited two social workers to carry out an NCD self-management program. A health care professional assisted social workers to complete six weekly two-hour sessions. The control group had no extra intervention. The evaluation was conducted at baseline and 12-months follow-up. Outcomes included changes in weight, blood pressure, fasting plasma glucose (FPG), lipid profile, self-management behaviors, and NCD-related knowledge. Results After 12 months, participants had gained 3.6 and 6.6 kg (P=0.025 for between-group difference) in the intervention and control groups, respectively. NCD-related knowledge increased by 15.4% and 11.3% (P<0.001 for between-group difference). The intervention group had a greater increase in self-reported exercise (87% to 95%) than the control group (81% to 85%) (P difference=0.031). General linear regression analysis showed that the intervention effects were significant for FPG (P=0.013) and total cholesterol (TC) (P=0.046).ConclusionsA social worker-led NCD self-management program successfully promoted NCD-related knowledge and decreased metabolic risk factors in low-income Cantonese women.Trial registrationChinese Clinical Trial Registry:Evaluation on effectiveness of Non-communicable Disease Self-management models led by social organization. ChiCTR1800015659. Registered 13 April 2018 - Retrospectively registered, http://www.chictr.org.cn/showproj.aspx?proj=15005
Does Baseline On-treatment Platelet Reactivity Impact Long-term Prognosis in Patients with Acute Coronary Syndrome and Thrombocytopenia Who Underwent Percutaneous Coronary Intervention?
Authors: Liu Ru , Li Tianyu , Yuan Deshan , Chen Yan , Tang Xiaofang , Gao Lijian , Zhang Ce , Jia Sida , Zhu Pei , Xu Ou , Gao Runlin , Xu Bo , Yuan Jjinqing 
Published: February 2021

Abstract
Objectives: This study analyzed the association between on-treatment platelet reactivity and long-term outcomes of patients with acute coronary syndrome (ACS) and thrombocytopenia (TP) in the real world. Methods: A total of 10724 consecutive cases with coronary artery disease who underwent percutaneous coronary intervention (PCI) were collected from January to December 2013. Cases with ACS and TP under dual anti-platelet therapy were enrolled from the total cohort. 5-year clinical outcomes were evaluated among cases with high on-treatment platelet reactivity (HTPR), low on-treatment platelet reactivity (LTPR) and normal on-treatment platelet reactivity (NTPR), tested by thromboelastogram (TEG) at baseline. Results: Cases with HTPR, LTPR and NTPR accounted for 26.2%, 34.4% and 39.5%, respectively. Cases with HTPR were presented with the most male sex, lowest hemoglobin level, highest erythrocyte sedimentation rate and most LM or three-vessel disease, compared with the other two groups. The rates of 5-year all-cause death, major adverse cardiovascular and cerebrovascular events (MACCE), cardiac death, myocardial infarction (MI), revascularization, stroke and bleeding were all not significantly different among three groups. Multivariable Cox regression indicated that, compared with cases with NTPR, cases with HTPR were not independently associated with all endpoints, as well as cases with LTPR (all P>0.05). Conclusions: In patients with ACS and TP undergoing PCI, 5-year all-cause death, MACCE, MI, revascularization, stroke and bleeding risk were all similar between cases with HTPR and cases with NTPR, tested by TEG at baseline, in the real world. The comparison result was the same between cases with LTPR and NTPR.
Bibliometric study of ‘overviews of systematic reviews’: evaluation of prevalence, citation and impact factor
Authors: Lunny Carole , Neelakant Trish , Chen Alyssa , Shinger Gavindeep , Stevens Adrienne , Tasnim Sara , Sadeghipouya Shadi , Adams Stephen , Zheng Yi Wen , Lin Lester , Yang Pei Hsuan , Dosanjh Manpreet , Ngsee Peter , Ellis Ursula , Shea Beverley J. , Reid Emma K. , Wright James M. 
Published: February 2021

Abstract
Background: Overviews synthesizing the results of multiple systematic reviews help inform evidence-based clinical practice. In this first of two companion papers, we evaluate the bibliometrics of ‘overviews of systematic reviews’, including their prevalence, number of citations, and factors affecting citation rates and journal impact factor.Methods: We searched MEDLINE, Epistemonikos and the Cochrane library databases. We applied eligibility criteria to identify overviews that: (a) aimed to focus on synthesizing reviews, (b) conducted a systematic search, (c) had a full methods section, and (d) examined a health intervention or clinical treatment effect. A multivariate regression was conducted to determine the association between citation density and impact factor and 6 predictor variables of interest. Results: We found 1218 overviews published from 2000 to 2020; the majority (73%) of which were published in the most recent 5-year period (2016-2020). We extracted a selection of these overviews (n=541; 44%) dated from 2000 to 2018. The 541 overviews were published in 307 journals; the Cochrane Database of Systematic Reviews (8%), PLOS ONE (3%) and the Sao Paulo Medical Journal (2%) being the most prevalent. The majority of overviews (70%) were published in journals with impact factors between 0.05 and 3.97. The average citation rate was 90 (SD ±219.7) over 9 years, or 10 citations per overview per year. In multivariate analysis, overviews with a high number of citations and high journal impact factors tended to have more authors, larger sample sizes, be open access and report funding source. Conclusions: We found an 8 fold increase in the number of overviews from 2009 to 2020; and a representation of one published a day in 2020. Factors driving the increase in overviews include the exponential increase in the number of systematic reviews, the publication of Cochrane guidance on overview of reviews in 2009 and the subsequent publication of the first Cochrane overview in the same year. Our study found a significantly higher mean citation count of 10 overviews per year, published in journals with a mean impact factor of 4.4. These data indicate that, overall, overviews perform above average for the journals in which they publish. We also found that highly cited overviews in high impact factor journals had group authorship, large sample sizes, were openly accessible, and reported funding source.
Effect of adhesive strategy on non-carious cervical lesions pre-conditioned with bioactive glass air-abrasion: a randomised controlled clinical trial
Authors: Milly Hussam , Karkoutly Humam , Banerjee Avijit 
Published: February 2021

Abstract
Background: This double-blind randomised controlled clinical trial evaluated the effect of adhesive strategy on the performance of resin composite restorations in non-carious cervical lesions (NCCLs) preconditioned using bioactive glass air-abrasion.Methods: One hundred teeth with NCCLs were assigned randomly into four experimental groups (n=25); A: air-abrasion + etch & rinse adhesive (AA+ER), B: air-abrasion + self-etch adhesive (AA+SE), C: etch & rinse adhesive (ER) and D: self-etch adhesive (SE). NCCLs were restored clinically using conventional resin composite placed incrementally. The lesions and restorations were evaluated at baseline, 3-months and 6-months according to the World Dental Federation (FDI) criteria. The level of statistical significance was established at p=0.05.Results: An increased retention rate was observed at 6-month recall within the AA+ER group. The retention rate of NCCLs restoration was affected significantly by the height of the NCCLs. NCCLs width and the height affected significantly the marginal adaptation of the restorations. Conclusion: Etch-and-rinse adhesive revealed a statistical improved retention rate when compared to self-etch adhesive in NCCLs pre-conditioned with bioactive glass air-abrasion at 6-months recall. NCCLs’ physical characteristics affected significantly the resin composite restoration performance.This clinical trial was registered in clinicaltrial.gov clinical registry under protocol # NCT04578717.