bluuebunny/crossref_metadata_2025_split huggingface dataset:
Heterogenous expression of endoglin marks advanced renal cancer with distinct tumor microenvironment fitness
Authors: Momoi Yusaku , Nishida Jun , Miyakuni Kosuke , Kuroda Masafumi , Kubota Shimpei I. , Miyazono Kohei , Ehata Shogo 
Published: June 2021

AbstractIntratumoral heterogeneity, including in clear cell renal cell carcinoma, is a potential cause of drug resistance and metastatic cancer progression. We specified the heterogeneous population marked by endoglin (also known as CD105) in a preclinical model of clear cell renal cell carcinoma progression. Highly malignant derivatives of human clear cell renal cell carcinoma OS‐RC‐2 cells were established as OS5Ks by serial orthotopic inoculation in our previous study. Expression of both ENG (encoding endoglin) mRNA and protein were heterogeneously upregulated in OS5Ks, and the endoglin‐positive (ENG+) population exhibited growth dependency on endoglin in anchorage‐independent cultures. Despite the function of endoglin as a type III receptor, transforming growth factor β and bone morphogenetic protein‐9 signaling were unlikely to contribute to the proliferative phenotype. Although endoglin has been proposed as a marker for renal cancer‐initiating cells, the OS5K‐3 ENG+ population did not enrich other reported cancer‐initiating cell markers or differentiate into the ENG– population. Mouse tumor inoculation models revealed that the tumor‐forming capabilities of OS5K‐3 ENG+ and ENG– cells in vivo were highly dependent on the microenvironment, with the renal microenvironment most preferable to ENG+ cells. In conclusion, the renal microenvironment, rather than the hypothesized ENG+ cell‐centered hierarchy, maintains cellular heterogeneity in clear cell renal cell carcinoma. Therefore, the effect of the microenvironment should be considered when evaluating the proliferative capability of renal cancer cells in the experimental settings.
Cognition and psychopathology in autoimmune encephalitides: A focus on risk factors and patient outcomes
Authors: Griffith Sarah P. , Malpas Charles B. , Kyndt Chris , Alpitsis Rubina , O’Brien Terence J. , Monif Mastura 
Published: June 2021

AbstractNeurocognitive compromise, neuropsychiatric symptoms and psychopathology are all evident in the acute stages of autoimmune encephalitides. These factors considerably affect functional independence after discharge. Drawing on psychometric assessments and qualitative descriptions, this review explores the nature, extent and diagnosis of cognitive disorder in autoimmune encephalitides. Potential pathophysiological and neuroanatomical architecture related to neurocognitive compromise in the acute and chronic stages of this illness is examined. In regard to outcomes, the review highlights clinicodemographic factors currently known to be associated with poorer cognitive outcome. Finally, the review delves into neuropsychiatric symptomology and psychological concerns that should be considered at diagnosis and during follow up of these patients.
Intraocular metastases of lung origin: Case reports and cytology correlates
Authors: Amer Hoda Zeinab M. , Cebulla Colleen M. , Li Zaibo , Shen Rulong 
Published: June 2021

AbstractIntraocular metastases from the lung are a common occurrence and an important differential for cytopathologists reading fine needle aspiration biopsies (FNAB). It is a particularly challenging diagnosis when the patient has had no previous diagnosis of lung cancer. We present two cases of intraocular metastases from lung primaries, and we discuss the clinical, radiological, and cytopathological features that help differentiate intraocular metastases of lung primary from other intraocular tumours, in the setting of FNAB. We also discuss the importance of recognising the spectrum of FNAB cases that can be seen specific to an institution, which may vary according to different patient populations. A thorough metastatic workup and ancillary testing, such as IHC or molecular genetics, ensures an accurate diagnosis.
Permanent loss of independence in adult febrile‐infection‐related epilepsy syndrome survivors: an underestimated and unsolved challenge
Authors: Nass Robert Daniel , Taube Julia , Bauer Tobias , Rüber Theodor , Surges Rainer , Helmstaedter Christoph 
Published: June 2021

AbstractBackground and purposeFebrile‐infection‐related epilepsy syndrome (FIRES) is an exceedingly rare and devastating subtype of new‐onset refractory status epilepticus, which causes refractory epilepsy and permanent neurocognitive impairment.MethodsThis was a long‐term follow‐up of adult FIRES survivors treated between 2005 and 2018 as part of the EpiCARE initiative, a European Reference Network for rare and complex epilepsies. Clinical, electroencephalography, imaging and functional outcome measures are described using the Scores of Independence for Neurologic and Geriatric Rehabilitation, the modified Rankin Scale and the Global Assessment of Severity of Epilepsy Scale.ResultsSix patients with refractory epilepsy following FIRES were evaluated. Despite general improvement after intensive care unit discharge, disease severity was still high at follow‐up in all patients. The functional outcome, as assessed by the modified Rankin Scale, was moderately impaired in 2/6 patients. In contrast, the Scores of Independence for Neurologic and Geriatric Rehabilitation indicated a loss of independence in 5/6, serious problems in memory and planning/problem‐solving in 4/6 and serious attentional problems in 3/6 patients.ConclusionsFebrile‐infection‐related epilepsy syndrome survivors may regain vital functions and mobility but experience a significant loss of independence and participation due to recurring seizures, structural brain damage and neurocognitive decline. Minimization of disastrous outcomes through the systematic evaluation of rescue therapies within a network of specialized centres is crucial.
Marine food web perspective to fisheries‐induced evolution
Authors: Hočevar Sara , Kuparinen Anna 
Published: June 2021

AbstractFisheries exploitation can cause genetic changes in heritable traits of targeted stocks. The direction of selective pressure forced by harvest acts typically in reverse to natural selection and selects for explicit life histories, usually for younger and smaller spawners with deprived spawning potential. While the consequences that such selection might have on the population dynamics of a single species are well emphasized, we are just beginning to perceive the variety and severity of its propagating effects within the entire marine food webs and ecosystems. Here, we highlight the potential pathways in which fisheries‐induced evolution, driven by size‐selective fishing, might resonate through globally connected systems. We look at: (i) how a size truncation may induce shifts in ecological niches of harvested species, (ii) how a changed maturation schedule might affect the spawning potential and biomass flow, (iii) how changes in life histories can initiate trophic cascades, (iv) how the role of apex predators may be shifting and (v) whether fisheries‐induced evolution could codrive species to depletion and biodiversity loss. Globally increasing effective fishing effort and the uncertain reversibility of eco‐evolutionary change induced by fisheries necessitate further research, discussion and precautionary action considering the impacts of fisheries‐induced evolution within marine food webs.
Polygenic risk scores for alcohol involvement relate to brain structure in substance‐naïve children: Results from the ABCD study
Authors: Hatoum Alexander S. , Johnson Emma C. , Baranger David A. A. , Paul Sarah E. , Agrawal Arpana , Bogdan Ryan 
Published: June 2021

AbstractBrain imaging‐derived structural correlates of alcohol involvement have largely been speculated to arise as a consequence of alcohol exposure. However, they may also reflect predispositional risk. In substance naïve children of European ancestry who completed the baseline session of the Adolescent Brain Cognitive Development (ABCD) Study (n = 3013), mixed‐effects models estimated whether polygenic risk scores (PRS) for problematic alcohol use (PAU‐PRS) and drinks per week (DPW‐PRS) are associated with magnetic resonance imaging‐derived brain structure phenotypes (i.e., total and regional: cortical thickness, surface area and volume; subcortical volume; white matter volume, fractional anisotropy, mean diffusivity). Follow‐up analyses evaluated whether any identified regions were also associated with polygenic risk among substance naïve children of African ancestry (n = 898). After adjustment for multiple testing correction, polygenic risk for PAU was associated with lower volume of the left frontal pole and greater cortical thickness of the right supramarginal gyrus (|βs| > 0.009; ps < 0.001; psfdr < 0.046; r2s < 0.004). PAU PRS and DPW PRS showed nominally significant associations with a host of other regional brain structure phenotypes (e.g., insula surface area and volume). None of these regions showed any, even nominal association among children of African ancestry. Genomic liability to alcohol involvement may manifest as variability in brain structure during middle childhood prior to alcohol use initiation. Broadly, alcohol‐related variability in brain morphometry may partially reflect predisposing genomic influence. Larger discovery genome‐wide association studies and target samples of diverse ancestries are needed to determine whether observed associations may generalize across ancestral origins.
Annual precipitation explains variability in dryland vegetation greenness globally but not locally
Authors: Ukkola Anna M. , De Kauwe Martin G. , Roderick Michael L. , Burrell Arden , Lehmann Peter , Pitman Andy J. 
Published: June 2021

AbstractDryland vegetation productivity is strongly modulated by water availability. As precipitation patterns and variability are altered by climate change, there is a pressing need to better understand vegetation responses to precipitation variability in these ecologically fragile regions. Here we present a global analysis of dryland sensitivity to annual precipitation variations using long‐term records of normalized difference vegetation index (NDVI). We show that while precipitation explains 66% of spatial gradients in NDVI across dryland regions, precipitation only accounts for <26% of temporal NDVI variability over most (>75%) dryland regions. We observed this weaker temporal relative to spatial relationship between NDVI and precipitation across all global drylands. We confirmed this result using three alternative water availability metrics that account for water loss to evaporation, and growing season and precipitation timing. This suggests that predicting vegetation responses to future rainfall using space‐for‐time substitution will strongly overestimate precipitation control on interannual variability in aboveground growth. We explore multiple mechanisms to explain the discrepancy between spatial and temporal responses and find contributions from multiple factors including local‐scale vegetation characteristics, climate and soil properties. Earth system models (ESMs) from the latest Coupled Model Intercomparison Project overestimate the observed vegetation sensitivity to precipitation variability up to threefold, particularly during dry years. Given projections of increasing meteorological drought, ESMs are likely to overestimate the impacts of future drought on dryland vegetation with observations suggesting that dryland vegetation is more resistant to annual precipitation variations than ESMs project.
Responses of soil organic carbon stock to animal manure application: A new global synthesis integrating the impacts of agricultural managements and environmental conditions
Authors: Li Binzhe , Song He , Cao Wenchao , Wang Yajing , Chen Jingsheng , Guo Jingheng 
Published: June 2021

AbstractEnhancing soil organic carbon (SOC) through applying animal manure is of interest for both sustaining cereal production and mitigating greenhouse gas (GHG) emissions. Previous syntheses showed that manuring‐induced SOC changes varied substantially with agricultural managements and environmental conditions, while their significance and relative importance to such variability are still largely uncertain. Here, we presented a new synthesis using an updated and balanced database integrating the manuring‐induced SOC stock changes and their plausible explanatory factors in 250 observations at global 120 sites. Manure application increased SOC stock by 7.41 ± 1.14 (95% confidence interval, CI) and 8.96 ± 1.83 (95% CI) Mg C ha−1, respectively, compared to their mineral fertilized (REF‐min) and unfertilized (REF‐zero) references. Of which approx. 72% and 34% were directly contributed by manure‐C input, respectively. Following the IPCC (Intergovernmental Panel on Climate Change) approach, these changes corresponded to the manuring‐induced SOC change factors of 1.27 ± 0.04 (95% CI) and 1.40 ± 0.08 (95% CI), respectively. Basing on a balanced database, we identified the amount of manure‐C input as the most important factor to the global variations in the resultant SOC stock changes. More importantly, our integrative analysis distinguished the significance of soil properties (e.g., soil pH and initial SOC content) in regulating the efficiency of manure application in enhancing SOC stock. These results indicate that, at the similar rate, applying manure could sequestrate much more carbon in alkaline soils than in neutral and acidic soils. By integrating the impacts of agricultural managements and environmental conditions, our findings would help to develop region‐specific tailor‐made manure application measures in agriculture and to refine the SOC change factors for regional GHG inventories.
Discriminating the biophysical signal from human‐induced effects on long‐term primary production dynamics. The case of Patagonia
Authors: Irisarri J. Gonzalo N. , Texeira Marcos , Oesterheld Martín , Verón Santiago R. , Della Nave Facundo , Paruelo José M. 
Published: June 2021

AbstractThe temporal trend of aboveground net primary production (ANPP) is frequently used to estimate the effect of humans on ecosystems. In water‐limited ecosystems, like most grazing areas in the world, the effect of humans act upon ANPP in combination with environmental variations. Our main objective was to quantify long‐term (1981–2012) changes of ANPP and discriminate the causes of these changes between environmental and human at a subcontinental scale, across vast areas of Patagonia. We estimated ANPP through a radiative model based on remote sensing data. Then, we evaluated the relation between ANPP and environmental interannual variations of two hierarchically related factors: El Niño Southern Oscillation (ENSO) through the Southern Oscillation Index (SOI), and precipitation. We described the effect of humans through the shape of the temporal trends of the residuals (RESTREND) of the environmental model and quantified human relative impact through the RESTREND: ANPP trend ratio. ANPP interannual variation was significantly explained by ENSO (through SOI) and precipitation in 65% of the study area. The SOI had a positive association with annual precipitation. The association between ANPP and annual precipitation was positive. RESTREND analysis was statistically significant in 92% of the area where the tested environmental model worked, representing 60% of the study area, and it was mostly negative. However, its magnitude, revealed through the RESTREND: ANPP trend ratio, was relatively mild. Our analysis revealed that most of ANPP trends were associated with climate and that even when human density is low, its incidence seems to be mainly negative.
Methane production and oxidation potentials along a fen‐bog gradient from southern boreal to subarctic peatlands in Finland
Authors: Zhang Hui , Tuittila Eeva‐Stiina , Korrensalo Aino , Laine Anna M. , Uljas Salli , Welti Nina , Kerttula Johanna , Maljanen Marja , Elliott David , Vesala Timo , Lohila Annalea 
Published: June 2021

AbstractMethane (CH4) emissions from northern peatlands are projected to increase due to climate change, primarily because of projected increases in soil temperature. Yet, the rates and temperature responses of the two CH4 emission‐related microbial processes (CH4 production by methanogens and oxidation by methanotrophs) are poorly known. Further, peatland sites within a fen‐bog gradient are known to differ in the variables that regulate these two mechanisms, yet the interaction between peatland type and temperature lacks quantitative understanding. Here, we investigated potential CH4 production and oxidation rates for 14 peatlands in Finland located between c. 60 and 70°N latitude, representing bogs, poor fens, and rich fens. Potentials were measured at three different temperatures (5, 17.5, and 30℃) using the laboratory incubation method. We linked CH4 production and oxidation patterns to their methanogen and methanotroph abundance, peat properties, and plant functional types. We found that the rich fen‐bog gradient‐related nutrient availability and methanogen abundance increased the temperature response of CH4 production, with rich fens exhibiting the greatest production potentials. Oxidation potential showed a steeper temperature response than production, which was explained by aerenchymous plant cover, peat water holding capacity, peat nitrogen, and sulfate content. The steeper temperature response of oxidation suggests that, at higher temperatures, CH4 oxidation might balance increased CH4 production. Predicting net CH4 fluxes as an outcome of the two mechanisms is complicated due to their different controls and temperature responses. The lack of correlation between field CH4 fluxes and production/oxidation potentials, and the positive correlation with aerenchymous plants points toward the essential role of CH4 transport for emissions. The scenario of drying peatlands under climate change, which is likely to promote Sphagnum establishment over brown mosses in many places, will potentially reduce the predicted warming‐related increase in CH4 emissions by shifting rich fens to Sphagnum‐dominated systems.
Transcriptomic exploration of a high sucrose mutant in comparison with the low sucrose mother genotype in sugarcane during sugar accumulating stage
Authors: Khan Qaisar , Chen Jiao‐Yun , Zeng Xu‐Peng , Qin Ying , Guo Dao‐Jun , Mahmood Amir , Yang Li‐Tao , Liang Qiang , Song Xiu‐Peng , Xing Yong‐Xiu , Li Yang‐Rui 
Published: June 2021

AbstractSugarcane (Saccharum spp. hybrid) is a noteworthy crop in the world for sugar and bioenergy production. In the present study, transcriptomic analysis was steered for a high sucrose mutant Guixuan B9 (GXB9) and the low sucrose mother genotype B9 of sugarcane during sugar accumulating stage. A total of 112,170 unigenes were obtained, and 106,026 (94.52%) were annotated by using the available public databases nonredundant, Gene ontology, COG, Kyoto Encyclopedia of Genes and Genomes, Swiss‐Prot, and Pfam. Functional assignment analysis of unigenes exhibited that they were active in a diversity of metabolic pathways. The vital unigenes for sucrose metabolism and accumulation were relatively characterized in the immature internodes (int. 5, 6) and maturing internodes (int. 13, 14) of GXB9 in comparison with B9. The differentially expressed unigenes (DEGs) encoding sucrose phosphate synthase (SPS, EC2.4.1.14), sucrose synthase (SuSy, EC2.4.1.13), and invertase (EC3.2.1.26), which are the vital enzymes functioning in the biosynthesis and splitting of sucrose, in the internodal tissues were revealed by using Illumina Hiseq 2000 platform, and verified by quantitative reverse transcription‐polymerase chain reaction analysis. The DEGs encoding SPS proteins SPS1, SPS2, SPS4, and SPS5 were upregulated in the maturing internodes of GXB9 compared with B9. The DEGs encoding SuSy proteins were majorly upregulated in the immature internodes of GXB9, and those encoding the enzymes involved in trehalose synthesis were downregulated in GXB9 compared with B9. The results in this study have revealed the opportunities for future sugarcane improvement by concentrating on the DEGs linked to sucrose metabolic pathways by applying omics technologies.
How much land does bioenergy require? An assessment for land‐scarce Switzerland
Authors: Bowman Gillianne , Burg Vanessa , Erni Matthias , Lemm Renato , Thees Oliver , Björnsen Gurung Astrid 
Published: June 2021

AbstractTo reduce greenhouse gas emissions, countries need to transform their energy system by increasing the share of renewable energies. For years, the use of fossil fuels meant devoting little land area to energy provision. As renewables require much more space, the relationship between renewable energy and land area becomes highly relevant. In this context, land scarcity is an important challenge, especially for densely populated countries. The power density concept, describing the relationship between energy carrier and area used for its production in W/m2, can aid decision‐making for resources allocation. Bioenergy plays a key role in the energy transition due to its diverse applications. Here, we assess how much area it takes to generate, transport and process various biomass types for energy purposes. We differentiate between 10 biomass types, determining area requirement (m2) and energy input (kWh) for each process along the supply chain. Using the whole sustainable biomass available requires >0.1% of Switzerland's land area (31 km2). Particularly for waste biomass, the area required for energy is negligible. Power densities vary widely within and between biomass types. Taking the average between minimum and maximum, they are highest for coniferous protection forest against natural hazards 114 W/m2 (22–267 W/m2) and green waste 96 W/m2 (26–176 W/m2). All of these are lower than literature values for fossil fuels (>1000 W/m2). However, sustainable power densities including compensatory land for greenhouse gas emissions are higher for biomass (average 2.4 W/m2, maximum 14.4 W/m2) than for fossil fuels (natural gas 0.9 W/m2, coal 0.2 W/m2). Estimating land requirement and power density facilitates weighing up whether and to what degree different biomass types should be used for energy.
Neighbourhood change and spatial inequalities in Cape Town
Authors: Lloyd Christopher D. , Bhatti Saad , McLennan David , Noble Michael , Mans Gerbrand 
Published: June 2021

AbstractCapturing the dynamics of population change in urban areas necessitates access to geographically fine‐grained and temporally consistent data for several time points. Such data are generally not available and they must be created using standard population data which cannot usually be compared across time periods. In this paper, the focus is on changing spatial inequalities in Cape Town, South Africa. This paper details an approach to generation of gridded population counts (250 m by 250 m) for two census years – 2001 and 2011. Census data for Small Area Layers (SALs), Spot Building Count (SBC) data, and Open Street Map (OSM) landuse data were used to construct a grid of populated cells to which population counts are then reallocated. The reallocation of population counts from SALs to grid cells was undertaken using area‐to‐point kriging – an approach which is informed by the spatial variation in the population groups of interest as measured using the variogram. A case study based on population grids of unemployment rates shows how the grids can be used to chart changes and also to measure spatial inequalities across the city at two time points. The advantages of grids for capturing fine‐scale complexities and correctly accounting for physical separation between communities are demonstrated and the results show, while the broad patterns of inequality are consistent across time, there are pronounced increases in inequalities in some neighbourhoods. These areas – and what leads some areas to fall further behind – should be the subject of attention by policy‐makers.