bluuebunny/crossref_metadata_2025_split huggingface dataset:
Numerical Simulation of Gas-Water Two-Phase Flow in Deep Shale Gas Reservoir Development Based on Mixed Fracture Modeling
Authors: Fang Sidong , Dai Cheng , Zeng Junsheng , Li Heng 
Published: November 2021

Abstract
In this paper, the development of a three-dimensional, two-phase fluid flow model (Modified Embedded Discrete Fracture Model) to study flow performances of a fractured horizontal well in deep-marine shale gas is presented. Deep-marine shale gas resources account for nearly 80% in China, which is the decisive resource basis for large-scale shale gas production. The dynamic characteristics of deep shale gas reservoirs are quite different and more complex. This paper uses the embedded discrete fracture model to simulate artificial fractures (main fractures and secondary fractures) and the dual-media model to simulate the mixed fractured media of natural fractures and considers the flow characteristics of partitions (artificial fractures, natural fractures, and matrix). Gas desorption is considered in the matrix. Different degrees of stress sensitivity are considered for natural and artificial fractures. Aiming at accurately simulating the whole production history of horizontal well fracturing, especially the dynamic changes of postfracturing flowback, a postfracturing fluid initialization method based on fracturing construction parameters (fracturing fluid volume and pump stop pressure) is established. The flow of gas and water in the early stage after fracturing is simulated, and the regional phase permeability and capillary force curves are introduced to simulate the process of flowback and production of horizontal wells after fracturing. The influence of early fracture closure on the gas-water flow is characterized by stress sensitivity. A deep shale gas reservoir of Sinopec was selected for the case study. The simulation results show it necessary to consider the effects of fractures and stress sensitivity in the matrix when considering the dynamic change of production during the flowback and production stages. The findings of this study can help for better understanding of the fracture distribution characteristics of shale gas, shale gas production principle, and well EUR prediction, which provide a theoretical basis for the effective development of shale gas horizontal well groups.
The Remediation Strategy and Mechanism of Combined Passivation and Foliar Inhibition for Safe Rice Production in Red Paddy Soil Contaminated With Heavy Metals
Authors: Zhang Fengsong , He Lixia , Zhang Guixiang , Wei Zhuo , Wang Jiaqi , Liu Hongfang , Wu Siyang 
Published: November 2021

Abstract
To solve the problem of Cd in rice over the food-safe standard, the present field study was carried out to explore the combined passivators (a mixture of quicklime (Q), polyacrylamide (A), sepiolite (S)) and Si-/Se- containing foliar inhibitors (Si or Se) at low (1) and high (2) application rates were added into the red paddy soil. After harvest the rice, the soil samples were collected to examine the soil properties, bacterial community, and the availability of heavy metals (Cd, Pb, and Cu) in soil. The rice samples were obtained to investigate the accumulation of heavy metals by rice. All of the treatments increased the soil's pH, but the treatments excluding QSe2 decreased the total P and soil organic matter (SOM), which was favourable for the immobilisation of heavy metals in red paddy soil by decreasing 14.29%-42.86% of available Cd, 10.18%-63.17% of available Pb, and 6.95%-36.81% of available Cu. With the increasing application rates, QAS significantly decreased the heavy metals available because of the enhanced immobilisation, while QSi and QSe significantly increased the heavy metals available because of the inhibited plant uptake. After remediation, QA1, QSi2, and QSe2 most effectively decreased the uptake Cd by rice in the present red paddy soil to solve the problem of Cd exceeding the threshold value according to the National Food Safety Standard of China (GB2762-2017). Additionally, the treatments, with the exception of Q1, QA1, QSi1, and QSi2, did not dramatically change the community structure of bacteria at the genus level in soil. Considering the safety and stability of soil, QSe2 was the primary recommendation for remediating Cd-contaminated red paddy soil.
The Role for GABAergic Interneurons Subtypes in the Antidepressant Efficacy of Traditional Chinese Medicine Formula Lily Bulb and Rehmannia Decoction
Authors: Xue Xiaoyan , Zhang Hongxiu , Pan Jin , Lu Yanting , Mao Qiancheng , Chang Xing , Wang Shijun , Song Xiaobin , Ma Ke 
Published: November 2021

Abstract
Background

Depression is associated with reduced γ-aminobutyric acid (GABA) and alterations of GABAergic interneurons in medial prefrontal cortex (mPFC), which can be normalized by traditional Chinese medicine formula lily bulb and Rehmannia decoction (LBRD) standard decoction. However, the impact of GABAergic interneurons subtypes including parvalbumin (PV), somatostatin (SST), and vasoactive intestinal peptide (VIP), in depressive episodes as well as in the LBRD standard decoction antidepressant effects remains to be elucidated.
Methods

We assessed the effects of LBRD standard decoction intervention on the expression of GABA associated miRNA/mRNA and GABAergic subtype-specific markers in chronic stress induced depression by western blot and qRT-PCR. The lncRNA/miRNA/GABA regulatory axis was verified by luciferase reporter assay, RNA immunoprecipitation, RNA pull-down assay and then detect its changes in LBRD standard decoction administration with use of immunofluorescence staining and RNA-fluorescence in situ hybridization (FISH).
Results

In the current study, we found that LBRD standard decoction exerted antidepressant effects based on the results of behavioral tests. Meanwhile, LBRD also revised the reduced GABA levels in depression by increasing the expression of lncRNA Neat1 and Malat1, as well as decreasing miRNA-144-3p and miRNA-15b-5p. Moreover, the expression of SST mRNA and protein in the mPFC tissues from depression group, was significantly lower than those in the control cases, while LBRD standard decoction treatment revised these changes. Whereas, neither chronic stress nor treatment can obviously change the level of PV and VIP mRNA and protein expression. In the SST-positive neurons of mPFC tissues, treatment with LBRD standard decoction resulted in the elevation of Gad-67, VGAT, GAT-3 and an reduction of miRNA-144-3p expression.
Conclusion

Taken together, these findings suggest that SST interneurons may be served as a preferentially vulnerable GABAergic neuronal subtype in depression and LBRD standard decoction antidepressant activities potentially relate to against the SST cells deficits by regulating the miRNA-144-3p mediated GABA synthesis, release.
Phenotypic characterization of four indigenous naked neck chicken ecotypes in Pakistan
Authors: Shafiq Muhammad , Khan Muhammad Tahir , Raziq Fazal , Bughio Erum , Farooq Zahid , Jalees Muhammad Mozam , Rauf Mubasher , Gondal Mushtaq Ahmad , Liaqat Saima , Sarwar Fozia , Azad Ayesha , Asad Tahreem , Arslan Muhammad , Azhar Muhammad , Kamal Rana Amjad 
Published: November 2021

Abstract
A study was conducted to characterize four phenotypes (black, white with black tips, light brown and dark brown) of naked neck chickens [n=320; 80(40♂, 40♀)] for qualitative and quantitative traits at 20 weeks of age. Qualitative phenotypic characterization was based on head appearance, comb type, wattle size, plumage pattern, shank color, spurs prevalence and number of toes. Quantitative phenotypic characterization was based on neck length, keel length, shank length, drum stick length, body length, wing span and circumference. Males and females of all phenotype had plain head and single comb. Plain feather pattern was predominantly most frequent on breast, wing bow, wing bar, wing bay, saddle and tail followed by stippled, penciled and laced. Males had most frequent yellow shanks followed by grey, off-white and green. White black, light and dark brown phenotypes expressed maximum yellow shank coloration whereas grey shanks were most frequent in black birds. Morpho-metric measurements were significantly higher in males than females. Light and dark brown phenotypes had higher values of quantitative traits than those of black and white black. All males and females of naked neck phenotypes possessed four toes, normal spurs and tuft feathers on the ventral portion of the neck above crop. The phenotypic variation observed in indigenous naked neck chickens could provide valuable basic information to develop effective utilization and conservation programs.
Statin in Combination with Xuezhikang Capsules Get More Lipid-Regulating Effects Than Statin Only: A Systematic Review and Meta-Analysis
Authors: Chen Dongxiong , Feng Huiyan , Li Yigen , Huang Jinzhu 
Published: November 2021

Abstract
Background

To compare the lipid-regulating effects and safety of statin combined with Xuezhikang capsules and statin used alone for hyperlipidemia.
Methods

CNKI, Wanfang database, VIP Chinese Journals, PubMed, Embase, and Cochrane library were searched to comprehensively collect and screen RCTs of Xuezhikang combined with statin compared with statin used alone for lipid modifying treatment in hyperlipidemia patients from the database built as of September 2021, and the Cochrane 5.1.0 quality evaluation form was used to evaluate the quality of the included literature and The Cochrane 5.1.0 quality assessment form was used to evaluate the quality of the included literature and bias analysis, extract basic study information, primary and secondary outcome indicators, and meta-analysis of the outcome indicators was performed using RevMan 5.3.
Results

A total of 14 studies with a total of 2042 patients were included, and the quality of the included studies was low to medium. ①Effectiveness rate: OR=3.63,95%CI[2.69,4.90]; Funnel plot: all within the funnel, but not in accordance with the principle of "aggregation at the top and dispersion at the bottom". ②Total cholesterol: MD=-0.61,95%CI[-0.84,-0.39], The forest plots of each subgroup are to the left. ③Triglycerides: MD=-0.30,95%CI[-0.41,-0.19], The forest plots of each subgroup are to the left,. ④ LDL: MD=-0.33,95%CI[-0.46,-0.20], The forest plots of each subgroup are to the left. ⑤ HDL: MD=0.23,95%CI[0.12,0.35], The forest plots of each subgroup are to the right. ⑥Adverse effects: OR=0.32, 95% CI[0.19,0.55].
Conclusion

The combination of statin with Xuezhikang capsule has better effect on lipid regulation in patients with hyperlipidemia, and can effectively reduce the incidence of adverse events and has better safety. It is recommended that the treatment of Xuezhikang combined with statin can be used as a safer and more effective treatment for patients with hyperlipidemia.
The HuoXue DiTan Recipe Attenuates Left Ventricular Hypertrophy in Spontaneously Hypertensive Rats by Increasing Autophagy Through the PTEN/PI3K/AKT/mTOR Pathway
Authors: Jiamei YAO , Cui ZHANG , Yushu YANG , Haiyan YANG , Dan ZHANG , Yongyue SHI , Qiong CHEN , Zhong Guangwei 
Published: November 2021

Abstract
Background

Hypertension-induced left ventricular hypertrophy (LVH) is associated with a reduction in autophagy, which can be inhibited by disruption of the PTEN/PI3K/AKT/mTOR pathway. The HuoXue DiTan recipe (HDR) is a commonly used prescription that has shown therapeutic effects on hypertension and its complications. However, its mechanisms are still unclear. In the present study, we hypothesized that HDR can regulate the PTEN/PI3K/AKT/mTOR signaling pathway and thereby reverse LVH by increasing autophagy in spontaneously hypertensive rats.
Methods

Twelve-week-old male spontaneously hypertensive (SH) rats and age-matched normotensive-control Wistar-Kyoto (WKY) rats were divided into four groups. After 12 weeks of treatment, echocardiographic measurements were made on the left ventricle, blood samples were collected for oxidative stress analysis, left ventricle tissue was processed for hematoxylin and eosin, Masson's trichrome, and immunohistochemical/ immunofluorescence staining, and TUNEL, RT-qPCR and Western blot analyses were performed.
Results

Compared with age-matched WKY rats, SH rats at 16 weeks of age exhibited significantly greater myocardial hypertrophy and remodeling with abnormal heart function. There was a reduction in autophagy and increase in apoptosis, resulting in an imbalance of oxidative stress manifested as left ventricular hypertrophy and impaired cardiac function. These effects may be related to a decrease in PTEN expression, which leads to activation of the PI3K/AKT/mTOR signaling pathway, resulting in abnormal expression of autophagy- and apoptosis-related proteins. After 12 weeks of HDR administration, blood pressure and ventricular hypertrophy were reduced, MDA and SOD levels and NADPH oxidase activity were better regulated, and gene expression of myocardial hypertrophy markers (ANP and β-MHC) was inhibited. HDR can promote autophagy and inhibit apoptosis, which may be related to regulation of autophagy- and apoptosis-related genes and proteins. HDR can also induce autophagy by enhancing expression of PTEN and inhibiting activation of the PI3K/AKT/mTOR signal pathway. Importantly, we demonstrated that VO-Ohpic, an inhibitor of PTEN, could suppress the effect of HDR on LVH in spontaneously hypertensive rats.
Conclusion

In conclusion, these results provide evidence for an important role of HDR in inhibition of left ventricular hypertrophy in spontaneously hypertensive rats, and indicate that it may act by improving autophagy through the PTEN/PI3K/AKT /mTOR pathway.
Parents’ Decision-making for Childhood Immunization and Prevention of Childhood Diseases – A Cross-Sectional Survey
Authors: NGANDJON Jonas KEMEUGNI , Laengler Alfred , Ostermann Thomas , Kenmoue Virgile 
Published: November 2021

Abstract
Background

The childhood vaccination program (EPI) is claimed by the World Health Organization (WHO) to be the most cost-effective intervention to reduce child mortality. Therefore, in low-income countries governments and health authorities invest in vaccination programs to reach herd immunity. However, despite the resources allocated to the EPI, epidemics preventable through vaccines are still reported in these countries. In Cameroon, the Foumbot district in the West region has witnessed measles epidemics since 2010 and in 2013 a polio outbreak was reported.
Methods

The design of this study is a cross-sectional survey. A total of 160 mothers of children between the ages of 12 to 23 months were selected by simple random sampling technique. Pre-tested structured questionnaire was used for data collection. Data was analyzed using SPSS statistical software.
Results

The outcome of this survey shows that 60% of the children studied were completely vaccinated, 37.75% were partially vaccinated, and 1.25% had not received any vaccine. The logistic regression analysis shows that a poor knowledge of infectious diseases (OR=0.3) was a significant predictor of partial and no vaccination status in children.
Conclusion

Parents’ decision-making for EPI was based on the information and experiences available in the community. Therefore, parents who are poorly educated on VPDs and living in a community with missing information and misinformation about vaccination will probably not complete the EPI. Public health authorities should invest in health education programs with the goal of developing skills for health- seeking behavior in individuals and communities.
Single Image Reconstruction using Novel Super Resolution Technique for Large Scaled Images
Authors: Datta Ramanath , Mandal Sekhar , Umer Saiyed , AlZubi Ahmad Ali , Alharbi Abdullah , Alanazi Jazem Mutared 
Published: November 2021

Abstract
A fast and novel method for single-image reconstruction using super resolution (SR) technique has been proposed in this paper. The working principle of proposed technique has been divided into three components. In the first component, a low resolution image is divided into several homogeneous or non-homogeneous regions. This partition is based on the analysis of texture pattern within that region. Only the non-homogeneous regions undergo to the sparse representation for super resolution image reconstruction in the second component. The obtained reconstructed region from the second component undergoes to a statistical based prediction model to generate its more enhanced version in the third component. The remaining homogeneous regions are bicubic interpolated and reflected to the required high resolution image. The proposed technique is applied on some Large scaled Electrical, Machine and Civil architectural design images. The purpose of using these images is that these images are huge in size and processing such large images for any applications, is time consuming. The proposed SR technique results the better reconstructed SR image from its very lower version with low time complexity. The performance of the proposed system on the Electrical, Machine and Civil architectural design images is compared with the state-of-the-art methods and it is shown that the proposed system outperforms the other competing methods.
Time-Dependent Effect of Graphene on the Microbial Activity of the Soil Under Single and Repeated Exposures
Authors: Liu Wenjuan , Guo Yufeng , Chai Guoli , Deng Wenbo 
Published: November 2021

Abstract
Graphene (GR) has huge industrial and biomedical potential, and its adverse effect on soil microorganisms has been evaluated in some ecotoxicological studies. These studies focus on a single exposure to GR, but repeated exposures are more likely to occur in soil. In this study, we compared the impact of single and repeated exposures (one, two and three exposures that resulted in the same final concentration) of GR on structure, abundance and function of soil bacterial community based on soil enzyme activity and high-throughput sequencing. The activities of urease and fluorescein diacetate esterase and alpha diversity demonstrate that repeated exposure to GR increase the diversity of soil bacterial diversity after 4 days of incubation following the last application of GR to soil. And the PCoA and sample level clustering tree showed single exposure to GR after 4 days alter the soil bacterial community to some extent, but the difference has been narrowed with the extension of time. During the entire incubation process, no matter what kind of exposure scenarios to GR, the majority of bacterial phylotypes remained unchanged except for Proteobacteria and Actinobacteria according to the relative abundance of phylotypes.
A dual-activity Topoisomerase Complex Regulates both Transcription and Translation
Authors: Su Shuaikun , Xue Yutong , Sharov Alexei , Zhang Yongqing , Lee Seung kyu , Martindale Jennifer , Li Wen , Ku Wai Lim , Zhao Keji , De Supriyo , Sen Payel , Gorospe Myriam , Xu Dongyi , Wang Weidong 
Published: November 2021

Abstract
Topoisomerases solve topological problems for DNA, but whether they function similarly for RNA remains unclear. Here we show that the Topoisomerase 3b (TOP3B)-TDRD3 complex acts on both DNA and RNA. On DNA, TOP3B-TDRD3 binds transcription start or termination regions; and inactivation of TOP3B topoisomerase activity decreases expression of some though not all genes downregulated in TOP3B-KO and TDRD3-KO cells. On mRNA, TOP3B-TDRD3 preferentially binds coding regions of long mRNAs and stabilizes them independently of TOP3B topoisomerase activity. Moreover, ablation of the complex or inactivation of TOP3B catalytic activity alters translation for some mRNAs, suggesting that TOP3B-TDRD3 may solve RNA topological problems. Finally, several schizophrenia and autism-risk genes are bound and regulated by TOP3B-TDRD3 at either transcription or translation. Our data suggest that TOP3B-TDRD3 can regulate transcription and translation by topoisomerase activity dependent or independent mechanisms; and disruption of either function could contribute to pathogenesis of psychiatric disorders in TOP3B mutation carriers.
Pueraria Flos Alleviates Alcoholic Liver Injury Via Regulation of PPARα and MAOA: Deciphering the Effective Forms and Potential Mechanism Based on a Bioinformatics-Integrated Metabolic Profile Strategy and Experimental Validation
Authors: Qu Jialin , Chen Qiuyue , Wei Tianfu , Dou Ning , Shang Dong , Yuan Dan 
Published: November 2021

Abstract
Background: Pueraria Flos, a representative medicinal and edible antidote for alcoholism, has rich clinical experience and remarkable curative effect in the treatment of alcoholic liver disease (ALD). However, its effective forms and hepatoprotective mechanism are remained unknown. Methods: A strategy based on UPLC-QTOF-MS combined with mass defect filtering technique was established for comprehensively identifying prototypes and metabolites absorbed and excreted into rat plasma, urine, bile and feces after oral administration. Then, the absorbed constituents with a relative high level were subjected to the network pharmacology, functional enrichment analysis and molecular docking to clarify the potential mechanism in the treatment of ALD. Furthermore, the therapeutic effect of PF on ALD and predicted mechanisms was further evaluated using a rat model of alcohol-induced liver injury and Western blot analysis. Results: 25 absorbed prototype constituents and 82 metabolites were identified or tentatively characterized with glucuronidation, sulfation, methylation, hydroxylation and reduction as their major metabolic pathways. The constructed absorbed constituent-target-pathway-disease network and docking analysis revealed that 4 metabolic components Te-7XG, genistein-7G-4'S, tectoridin-4'S and Te-7XG-4'S, 2 targets MAOA and PPARA, and 6 pathways related to lipid regulation and amino acid metabolism may play crucial roles in the PF mediated protection against ALD. An in vivo validation in rat further demonstrated that PF alleviated liver injury via activating and suppressing the PPARA and MAOA expression, respectively. Conclusions: The present results not only increase the understanding on the effective form and molecular mechanism of PF mediated protection against ALD, but also promote better application of PF as supplement food and herbal medicine for the treatment of ALD.