bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Expanding Coal Power Fleet in Southeast Asia: Implications for Future CO2 Emissions and Electricity Generation
Authors: Chen Xu , Mauzerall Denise L. 
Published: November 2021

AbstractCoal combustion for power generation made up 30% of global CO2 emissions in 2018. To achieve the goal of the Paris Agreement to keep global average temperatures below 2°C, power generation must be decarbonized globally by mid‐century. This requires a rapid phase‐out of coal‐fired power generation. However, global coal power expansion continues, mostly in developing countries where electricity demand continues to increase. Since the early 2010s, Southeast Asia's coal power capacity expansion has been among the fastest in the world, following China and India, but its implications for the global climate and regional energy transition remain understudied. Here we examine Southeast Asia's power generation pipeline as of mid‐2020 and evaluate its implications for the region's CO2 emissions over the plant lifetime as well as projected electricity generation between 2020‐2030 in Indonesia, Vietnam, and the Philippines. We find that power plants under construction and planned in Southeast Asia as of 2020 will more than double the region's fossil fuel power generation capacity. If all fossil fuel plants under development are built, Southeast Asia's power sector CO2 emissions will increase by 72% from 2020 to 2030 and long‐term committed emissions will double. Moreover, in Indonesia, Vietnam, and the Philippines, projected electricity generation from fossil fuel plants under development, combined with generation from renewable capacity targets and existing power capacity, will exceed future national electricity demand. As a result, fossil fuel plants will likely be underutilized and/or become stranded assets while also potentially crowding out renewable energy deployment.
Controls on Alkenone Carbon Isotope Fractionation in the Modern Ocean
Authors: Phelps Samuel R. , Stoll Heather M. , Bolton Clara T. , Beaufort Luc , Polissar Pratigya J. 
Published: November 2021

AbstractCarbon isotope records from alkenone biomarkers (εp37:2) produced by haptophyte algae are frequently used for atmospheric CO2 paleobarometry, but this method has yielded inconsistent results during periods where CO2 variations are known independently. Recent syntheses of algal cultures have quantitatively demonstrated that εp37:2 indeed records CO2 information: εp37:2 increases as aqueous CO2 concentrations increase relative to carbon demand. However, interpretations of εp37:2 are complicated by irradiance, where higher irradiance yields higher εp37:2. Here we examine the roles of physiology and environment in setting εp37:2 in the ocean. We compile water‐column and sediment core‐top εp37:2 data and add new core‐top measurements, including estimates of cell sizes and growth rates of the alkenone‐producing population. In support of culture studies, we find irradiance to be a key control on εp37:2 in the modern ocean. We test a culture‐derived model of εp37:2 and find that the quantitative relationships calibrated in culture experiments can be used to predict εp37:2 in sediment samples. In water‐column samples, the model substantially overestimates εp37:2, largely resulting from higher irradiance at the depth of sample collection than the integrated light conditions under which the sampled biomass was produced and vertically mixed to the collection depth. We argue that the theory underpinning the conventional diffusive alkenone carbon isotope fractionation model, including the “b” parameter, is not supported by field data and should not be used to reconstruct past CO2 changes. Future estimates of CO2 from εp37:2 should use empirical or mechanistic models to quantitatively account for irradiance and cell size variations.
Optimal Precursors Triggering Sudden Shifts in the Antarctic Circumpolar Current Transport Through Drake Passage
Authors: Zhou Li , Wang Qiang , Mu Mu , Zhang Kun 
Published: November 2021

AbstractDue to the high nonlinearity, the accurate prediction of the Antarctic circumpolar current (ACC) transport is still challenging. Using the eddy‐permitting regional ocean modeling system and the conditional nonlinear optimal perturbation approach, the sudden shift in the ACC transport through Drake Passage (DP) is investigated by exploring its optimal precursor (OPR). Here, the sudden shift in the ACC transport is defined as a fluctuation exceeding double STD (∼16 Sv; 1 Sv = 106 m3 s−1) within 30 days. The results indicate that the OPRs for all three cases exhibit specific structures in the middle DP (58°–62°S, 72°–64°W) at the depth of 1,000–3,000 m, implying that the ACC transport is most sensitive to the initial perturbations there. The OPRs' evolutions show similar features: the OPR for each case triggers an eddy‐like dipole perturbation with a northern cyclone and a southern anticyclone, leading to a sudden reduction in the ACC transport of ∼40 Sv. It is verified that the density components in the OPRs determine such evolution processes. Furthermore, baroclinic instability is diagnosed to play a dominant role in the development of OPRs by analyzing the perturbed kinetic energy budget. This study suggests that the deep‐layer density perturbations in the Southern Ocean should be carefully monitored when considering the short‐range prediction of the ACC transport.
BOD as a Measure of Fluvial Organic Matter Lability—The Decoupling of O2 Consumption From CO2 Production
Authors: Worrall Fred , Howden Nicholas J. K. , Burt Tim P. 
Published: November 2021

AbstractBiochemical oxygen demand (BOD) has been taken as a measure of the lability of the fluvial organic matter (both dissolved and particulate organic matter—DOM and POM), but such a link is based on a number of assumptions. First, the oxidation state of the organic carbon is zero and the molar stoichiometry of the reaction between organic C and O2 is 1. Second, the consumption of O2, as measured by BOD, is a first‐order reaction with respect to DOM or POM. Third, that POM and DOM are correlated. Applying a Bayesian hierarchical analysis to an extensive river monitoring records for 236 sites across Great Britain from 1974 to 2019, it is possible to test the nature of the link between BOD and fluvial organic matter. The study shows that: (a) The inclusion of both DOC and SS concentrations improved the modeling. (b) When a first‐order reaction was assumed there was no significant linear relationship with BOD and DOC and suspended solids (SS) in at least 98% of cases. (c) 53% of sites showed a zero‐order reaction; when there was a significant order of reaction, the median was 0.28 and was never larger than 0.93, that is, a first‐order reaction rate was never observed. The lack of significant relationship means that the supply of organic matter exceeds the capacity to turn over that organic matter; and that given the range of oxidation states of fluvial organic carbon, it is possible to completely decouple O2 consumption from fluvial organic matter concentration in rivers.
Regulation of antiviral innate immune signaling and viral evasion following viral genome sensing
Authors: Chathuranga Kiramage , Weerawardhana Asela , Dodantenna Niranjan , Lee Jong-Soo 
Published: November 2021

AbstractA harmonized balance between positive and negative regulation of pattern recognition receptor (PRR)-initiated immune responses is required to achieve the most favorable outcome for the host. This balance is crucial because it must not only ensure activation of the first line of defense against viral infection but also prevent inappropriate immune activation, which results in autoimmune diseases. Recent studies have shown how signal transduction pathways initiated by PRRs are positively and negatively regulated by diverse modulators to maintain host immune homeostasis. However, viruses have developed strategies to subvert the host antiviral response and establish infection. Viruses have evolved numerous genes encoding immunomodulatory proteins that antagonize the host immune system. This review focuses on the current state of knowledge regarding key host factors that regulate innate immune signaling molecules upon viral infection and discusses evidence showing how specific viral proteins counteract antiviral responses via immunomodulatory strategies.
Histone N-terminal acetyltransferase NAA40 links one-carbon metabolism to chemoresistance
Authors: Demetriadou Christina , Raoukka Anastasia , Charidemou Evelina , Mylonas Constantine , Michael Christina , Parekh Swati , Koufaris Costas , Skourides Paris , Papageorgis Panagiotis , Tessarz Peter , Kirmizis Antonis 
Published: November 2021

AbstractAberrant function of epigenetic modifiers plays an important role not only in the progression of cancer but also the development of drug resistance. N-alpha-acetyltransferase 40 (NAA40) is a highly specific epigenetic enzyme catalyzing the transfer of an acetyl moiety at the N-terminal end of histones H4 and H2A. Recent studies have illustrated the essential oncogenic role of NAA40 in various cancer types but its role in chemoresistance remains unclear. Here, using transcriptomic followed by metabolomic analysis in colorectal cancer (CRC) cells, we demonstrate that NAA40 controls key one-carbon metabolic genes and corresponding metabolites. In particular, through its acetyltransferase activity NAA40 regulates the methionine cycle thereby affecting global histone methylation and CRC cell survival. Importantly, NAA40-mediated metabolic rewiring promotes resistance of CRC cells to antimetabolite chemotherapy in vitro and in xenograft models. Specifically, NAA40 stimulates transcription of the one-carbon metabolic gene thymidylate synthase (TYMS), whose product is targeted by 5-fluorouracil (5-FU) and accordingly in primary CRC tumours NAA40 expression associates with TYMS levels and poorer 5-FU response. Mechanistically, NAA40 activates TYMS by preventing enrichment of repressive H2A/H4S1ph at the nuclear periphery. Overall, these findings define a novel regulatory link between epigenetics and cellular metabolism mediated by NAA40, which is harnessed by cancer cells to evade chemotherapy.
Network based analysis identifies TP53m-BRCA1/2wt-homologous recombination proficient (HRP) population with enhanced susceptibility to Vigil immunotherapy
Authors: Sliheet Elyssa , Robinson Molly , Morand Susan , Choucair Khalil , Willoughby David , Stanbery Laura , Aaron Phylicia , Bognar Ernest , Nemunaitis John 
Published: November 2021

AbstractThus far immunotherapy has had limited impact on ovarian cancer. Vigil (a novel DNA-based multifunctional immune-therapeutic) has shown clinical benefit to prolong relapse-free survival (RFS) and overall survival (OS) in the BRCA wild type and HRP populations. We further analyzed molecular signals related to sensitivity of Vigil treatment. Tissue from patients enrolled in the randomized double-blind trial of Vigil vs. placebo as maintenance in frontline management of advanced resectable ovarian cancer underwent DNA polymorphism analysis. Data was generated from a 981 gene panel to determine the tumor mutation burden and classify variants using Ingenuity Variant Analysis software (Qiagen) or NIH ClinVar. Only variants classified as pathogenic or likely pathogenic were included. STRING application (version 1.5.1) was used to create a protein-protein interaction network. Topological distance and probability of co-mutation were used to calculated the C-score and cumulative C-score (cumC-score). Kaplan–Meier analysis was used to determine the relationship between gene pairs with a high cumC-score and clinical parameters. Improved relapse free survival in Vigil treated patients was found for the TP53m-BRCAwt-HRP group compared to placebo (21.1 months versus 5.6 months p = 0.0013). Analysis of tumor mutation burden did not reveal statistical benefit in patients receiving Vigil versus placebo. Results suggest a subset of ovarian cancer patients with enhanced susceptibility to Vigil immunotherapy. The hypothesis-generating data presented invites a validation study of Vigil in target identified populations, and supports clinical consideration of STRING-generated network application to biomarker characterization with other cancer patients targeted with Vigil.
Mechanism of transient photothermal inactivation of bacteria using a wavelength-tunable nanosecond pulsed laser
Authors: Tatsuno Ichiro , Niimi Yuna , Tomita Makoto , Terashima Hiroshi , Hasegawa Tadao , Matsumoto Takahiro 
Published: November 2021

AbstractThere is a great demand for novel disinfection technologies to inactivate various pathogenic viruses and bacteria. In this situation, ultraviolet (UVC) disinfection technologies seem to be promising because biocontaminated air and surfaces are the major media for disease transmission. However, UVC is strongly absorbed by human cells and protein components; therefore, there are concerns about damaging plasma components and causing dermatitis and skin cancer. To avoid these concerns, in this study, we demonstrate that the efficient inactivation of bacteria is achieved by visible pulsed light irradiation. The principle of inactivation is based on transient photothermal heating. First, we provide experimental confirmation that extremely high temperatures above 1000 K can be achieved by pulsed laser irradiation. Evidence of this high temperature is directly confirmed by melting gold nanoparticles (GNPs). Inorganic GNPs are used because of their well-established thermophysical properties. Second, we show inactivation behaviour by pulsed laser irradiation. This inactivation behaviour cannot be explained by a simple optical absorption effect. We experimentally and theoretically clarify this inactivation mechanism based on both optical absorption and scattering effects. We find that scattering and absorption play an important role in inactivation because the input irradiation is inherently scattered by the bacteria; therefore, the dose that bacteria feel is reduced. This scattering effect can be clearly shown by a technique that combines stainedEscherichia coliand site selective irradiation obtained by a wavelength tunable pulsed laser. By measuring Live/Dead fluorescence microscopy images, we show that the inactivation attained by the transient photothermal heating is possible to instantaneously and selectively kill microorganisms such asEscherichia colibacteria. Thus, this method is promising for the site selective inactivation of various pathogenic viruses and bacteria in a safe and simple manner.
Optimization of anti-ADAMTS13 antibodies for the treatment of ADAMTS13-related bleeding disorder in patients receiving circulatory assist device support
Authors: Ito Toshihiro , Minamitani Takeharu , Hayakawa Masaki , Otsubo Ryota , Akiba Hiroki , Tsumoto Kouhei , Matsumoto Masanori , Yasui Teruhito 
Published: November 2021

AbstractADAMTS13 (a disintegrin-like and metalloproteinase with thrombospondin type-1 motif 13)-related bleeding disorder has been frequently observed as a life-threatening clinical complication in patients carrying a circulatory assist device. Currently, treatment modalities for the bleeding disorder are very limited and not always successful. To address the unmet medical need, we constructed humanized antibodies of mouse anti-ADAMTS13 antibody A10 (mA10) by using complementarity-determining region (CDR) grafting techniques with human antibody frameworks, 8A7 and 16E8. The characteristics of the two humanized A10 antibodies, namely A10/8A7 and A10/16E8, were assessed in vitro and in silico. Among the two humanized A10 antibodies, the binding affinity of A10/16E8 to ADAMTS13 was comparable to that of mA10 and human-mouse chimeric A10. In addition, A10/16E8 largely inhibited the ADAMTS13 activity in vitro. The results indicated that A10/16E8 retained the binding affinity and inhibitory activity of mA10. To compare the antibody structures, we performed antibody structure modeling and structural similarity analysis in silico. As a result, A10/16E8 showed higher structural similarity to mA10, compared with A10/8A7, suggesting that A10/16E8 retains a native structure of mA10 as well as its antigen binding affinity and activity. A10/16E8 has great potential as a therapeutic agent for ADAMTS13-related bleeding disorder.
Processing of fMRI-related anxiety and bi-directional information flow between prefrontal cortex and brain stem
Authors: Pfurtscheller Gert , Blinowska Katarzyna J. , Kaminski Maciej , Schwerdtfeger Andreas R. , Rassler Beate , Schwarz Gerhard , Klimesch Wolfgang 
Published: November 2021

AbstractBrain–heart synchronization is fundamental for emotional-well-being and brain–heart desynchronization is characteristic for anxiety disorders including specific phobias. Recording BOLD signals with functional magnetic resonance imaging (fMRI) is an important noninvasive diagnostic tool; however, 1–2% of fMRI examinations have to be aborted due to claustrophobia. In the present study, we investigated the information flow between regions of interest (ROI’s) in the cortex and brain stem by using a frequency band close to 0.1 Hz. Causal coupling between signals important in brain–heart interaction (cardiac intervals, respiration, and BOLD signals) was studied by means of Directed Transfer Function based on the Granger causality principle. Compared were initial resting states with elevated anxiety and final resting states with low or no anxiety in a group of fMRI-naïve young subjects. During initial high anxiety the results showed an increased information flow from the middle frontal gyrus (MFG) to the pre-central gyrus (PCG) and to the brainstem. There also was an increased flow from the brainstem to the PCG. While the top-down flow during increased anxiety was predominant, the weaker ascending flow from brainstem structures may characterize a rhythmic pacemaker-like activity that (at least in part) drives respiration. We assume that these changes in information flow reflect successful anxiety processing.
Microstructural, electrical and biological activity in $$\mathrm{Ca}_{10}(\mathrm{PO}_4)_6(\mathrm{OH})_2-\mathrm{Ba}_{0.5}\mathrm{Sr}_{0.5}\mathrm{TiO}_3$$ ceramic composites designed for tissue engineering applications
Authors: Das Apurba , Dobbidi Pamu , Bhardwaj Aman , Saxena Varun , Pandey Lalit M. 
Published: November 2021

AbstractThe article investigates electrically active ceramic composite of $$\mathrm{Ca}_{10}(\mathrm{PO}_4)_6(\mathrm{OH})_2$$

Ca
10

(

PO
4

)

6

(
OH
)

2

(HAP) and $$\mathrm{Ba}_{0.5}\mathrm{Sr}_{0.5}\mathrm{TiO}_{3}$$

Ba

0.5

Sr

0.5

TiO
3

(BST) for biomedical applications. The study is a systematic blend of the materials science aspect of composites with a special focus on the dielectric and biological properties and their relationships. The article emphasized primarily extracting the dielectric constant ($$\epsilon _r)$$

ϵ
r

)

of the specimens (that lay in the range of 3–65) and related them to microstructural properties like the grain size and at.% of BST. A broad outlook on the importance of $$\epsilon _r$$

ϵ
r

in determining the suitability of bioceramics for clinical applications is presented. Bioactivity analysis of the specimens led to probing the surface charges (that were negative), and it was found crucial to the growth of dense apatite layers. Furthermore, the cytocompatibility of the specimens displayed cell viability above 100% for Day 1, which increased substantially for Day 3. To reveal other biological properties of the composites, protein adsorption studies using bovine serum albumin (BSA) and fetal bovine serum (FBS) was carried out. Electrostatic interactions govern the adsorption, and the mathematical dependence on surface charges is linear. The protein adsorption is also linearly correlated with the $$\epsilon _r$$

ϵ
r

, intrinsic to the biomaterials. We delve deeper into protein–biomaterials interactions by considering the evolution of the secondary structure of BSA adsorbed into the specimens. Based on the investigations, 20 at.% HAP–80 at.% BST (20H–80B) was established as a suitable composite comprising the desired features of HAP and BST. Such explorations of electrical and biological properties are interesting for modulating the behavior of bioceramic composites. The results project the suitability of 20H–80B for designing electrically active smart scaffolds for the proposed biomedical applications and are expected to incite further clinical trials.
Activation of specific bitter taste receptors by olive oil phenolics and secoiridoids
Authors: Cui Meng , Chen Bohan , Xu Keman , Rigakou Aimilia , Diamantakos Panagiotis , Melliou Eleni , Logothetis Diomedes E. , Magiatis Prokopios 
Published: November 2021

AbstractExtra-virgin olive oil (EVOO) is a critical component of the Mediterranean diet, which has been found beneficial to human health. Bitterness is often positively associated with the presence of phenolic compounds in EVOO. There are twenty-five bitter taste receptors (TAS2Rs) in humans, each of which responds to specific bitter tastants. The identity of phenolic compounds and the bitter taste receptors they stimulate remain unknown. In this study, we isolated 12 phenolic and secoiridoid compounds from the olive fruit and the oil extracted from it, and tested their ability to stimulate bitter taste receptor activity, using a calcium mobilization functional assay. Our results showed that seven out of twelve studied compounds activated TAS2R8, and five of them activated TAS2R1, TAS2R8, and TAS2R14. The phenolic compounds oleuropein aglycon and ligstroside aglycon were the most potent bitter tastants in olive oil. TAS2R1 and TAS2R8 were the major bitter taste receptors activated most potently by these phenolic compounds. The results obtained here could be utilized to predict and control the bitterness of olive oil based on the concentration of specific bitter phenolics produced during the milling process of olives.
Driving electrochemical corrosion of implanted CoCrMo metal via oscillatory electric fields without mechanical wear
Authors: Welles Thomas S. , Ahn Jeongmin 
Published: November 2021

AbstractDecades of research have been dedicated to understanding the corrosion mechanisms of metal based implanted prosthetics utilized in modern surgical procedures. Focused primarily on mechanically driven wear, current fretting and crevice corrosion investigations have yet to precisely replicate the complex chemical composition of corrosion products recovered from patients’ periprosthetic tissue. This work specifically targets the creation of corrosion products at the metal on metal junction utilized in modular hip prosthetics. Moreover, this manuscript serves as an initial investigation into the potential interaction between implanted CoCrMo metal alloy and low amplitude electrical oscillation, similar in magnitude to those which may develop from ambient electromagnetic radiation. It is believed that introduction of such an electrical oscillation may be able to initiate electrochemical reactions between the metal and surrounding fluid, forming the precursor to secondary wear particles, without mechanically eroding the metal’s natural passivation layer. Here, we show that a low magnitude electrical oscillation (≤ 200 mV) in the megahertz frequency (106 Hz) range is capable of initiating corrosion on implanted CoCrMo without the addition of mechanical wear. Specifically, a 50 MHz, 200 mVpp sine wave generates corrosion products comprising of Cr, P, Ca, O, and C, which is consistent with previous literature on the analysis of failed hip prosthetics. These findings demonstrate that mechanical wear may not be required to initiate the production of chemically complex corrosion products.