bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comprehensive Analysis of the Autophagy-dependent Ferroptosis-related Gene FANCD2 in Lung Adenocarcinoma
Authors: Miao Huikai , Ren Qiannan , Li Hongmu , Zeng Mingyue , Chen Dongni , Chen Youfang , Wen Zhesheng 
Published: July 2021

Abstract
Background. The development of lung adenocarcinoma (LUAD) involves the interactions between cell proliferation and death. Autophagy-dependent ferroptosis, a distinctive cell death process was implicated in a multitude of diseases, whereas no research revealing the relationship between autophagy-dependent ferroptosis and LUAD pathogenesis was reported. Thus, the primary objective was to explore the role and potential function of the autophagy-dependent ferroptosis-related genes in LUAD.Methods. Clinical information and transcriptome profiling of patients with LUAD were retrieved and downloaded from open-source databases. Autophagy-dependent ferroptosis-related genes were screened by published articles. The key gene was identified as the intersection between the differentially expressed genes and prognosis-related genes. Patients were divided into high- and low-risk groups using the expression level of the key gene. The validity of the key gene prognosis model was verified by survival analysis. The correlation between the clinical characteristics of LUAD and the expression level of the key gene was analyzed to explore the clinical significance and prognosis value. And the roles of the key gene in response to chemotherapy, immune microenvironment and tumor mutation burden were predicted. The validation of key gene expression levels were further performed by quantitative real-time PCR and immunohistochemistry staining.Results. FANCD2, a key autophagy-dependent ferroptosis-related gene by searching database, was confirmed as an independent prognostic factor for LUAD occurrence. The high expression level of FANCD2 was associated with an advantaged TNM stage, a less chemotherapy sensitivity, a low ImmuneScore, which indicated a deactivation status in immune microenvironment, a high tumor mutation burden, and a poor survival for LUAD patients. Pathway enrichment analysis showed that FANCD2 were involved in response to oxidative stress and neutrophil mediated immunity. Quantitative real-time PCR and immunohistochemistry staining showed that the expression level of FANCD2 is higher in LUAD patients than normal tissue samples, which was in accordance with the database report.Conclusion. FANCD2, a key gene related to autophagy-dependent ferroptosis, could work as a biomarker, which predicts the survival, chemotherapy sensitivity, tumor immunity and mutation burden of LUAD. Research of autophagy-dependent ferroptosis and targeting the FANCD2 may offer a new perspective for the treatment and improvement of prognosis in LUAD.
Blood Circulatory Level of Seven Sirtuins in Alzheimer’s Disease: Potent Biomarker Based on Translational Research
Authors: Pradhan Rashmita , Singh Abhinay Kumar , Kumar Pramod , Bajpai Swati , Pathak Mona , Chatterjee Prasun , Dwivedi Sadanand , Dey A.B. , Dey Sharmistha 
Published: July 2021

Abstract
Alzheimer's disease (AD) is an accelerating neurodegenerative disorder. Dysfunction of mitochondria and oxidative stress contributes to the pathogenesis of AD. Sirtuins play a role in this pathway and can be a potential marker to study neurodegenerative changes. This study evaluated serum levels of all seven sirtuins (SIRT1 – SIRT7) proteins in three study groups: AD, Mild Cognitive Impairment (MCI), and Geriatric Control (GC) by Surface Plasmon Resonance (SPR) technique. Further, it was validated by the Western blot experiment. ROC analysis was performed to differentiate the study group based on the concentration of serum SIRT proteins. Out of seven sirtuins, serum SIRT1, SIRT3, and SIRT6 levels (mean+SD) were significantly decreased in AD (1.65±0.56, 3.15±0.28, 3.36±0.32ng/µl); compare to MCI (2.17±0.39, 3.60±0.51, 3.73±0.48ng/µl) and GC (2.84±0.47, 4.55±0.48, 4.65±0.55ng/µl). ROC analysis showed the cut-off value with high sensitivity and specificity for cognitive impairment (AD and MCI). The concentration declined significantly with the disease progression. No specific difference was observed in case of other SIRTS between the study groups. This study reveals an inverse relation of serum SIRT1, SIRT3, and SIRT6 concentration with AD. ROC analysis showed that these serum proteins have greater accuracy in diagnosing of AD. This is the first report of estimation of all seven serum sirtuins and the clinical relevance of SIRT3 and SIRT6 as serum protein markers for AD.
Amyloid PET Quantification Using Low-dose CT-guided Anatomic Standardization
Authors: Matsuda Hiroshi , Yamao Tensho , Shakado Mitsuru , Shigemoto Yoko , Okita Kyoji , Sato Noriko 
Published: July 2021

Abstract
BackgroundCentiloid (CL) scaling has become a standardized quantitative measure in amyloid PET because it facilitates the direct comparison of results across institutions, even when different analytical methods or tracers are used. Standard volumes of interest must be used to calculate the CL scale after the anatomic standardization of amyloid PET images using coregistered MRI; if the MRI is unavailable, the CL scale cannot be accurately calculated. This study sought to determine the substitutability of low-dose CT, which is used to correct PET attenuation in PET/CT equipment, by evaluating the measurement accuracy when low-dose CT is used as an alternative to MRI in the calculation of the CL scale. Amyloid PET images obtained using 18F-flutemetamol from 24 patients with possible or probable Alzheimer’s disease were processed to calculate the CL scale using 3D T1-weighted MRI and low-dose CT of PET/CT. CLMRI and CLCT were respectively defined as the use of MRI and CT for anatomic standardization and compared. Trial registration: Japan Registry of Clinical Trials, jRCTs031180321. Registered 18 March 2019, https://jrct.niph.go.jp/latest-detail/jRCTs031180321.ResultsA Bland–Altman plot showed that CLCT was slightly but significantly underestimated (mean ± standard deviation, −1.7 ± 2.4; p < 0.002) compared with CLMRI. The 95% limits of agreement ranged from −2.8 to −0.7. Pearson correlation analysis showed a highly significant correlation of r = 0.998 between CLCT and CLMRI (p < 0.001). The linear regression equation was CLMRI = 1.027 × CLCT + 0.762. In a Bland-Altman plot, Spearman correlation analysis did not identify a significant association between the CLMRI versus CLCT difference and the CL load (ρ = −0.389, p = 0.060). This slight underestimation of CLCT may derive from slightly higher uptake when the cerebellum is used as a reference area in CT-based anatomically standardized PET images versus MRI-based images.ConclusionsLow-dose CT of PET/CT can substitute for MRI in the anatomic standardization used to calculate the CL scale from amyloid PET, although a slight underestimation occurs.
Inverted ternary OPD based on PEIE
Authors: An Tao , Liu Suiyang 
Published: July 2021

Abstract
This paper proposes an inverted ternary organic photodetector (OPD), whose structure is ITO/PEIE/PC61BM/P3HT:PCPDTBT/MoO3 /Al. The use of PEIE as the cathode buffer layer avoids the influence of acidic PEDOT:PSS on the surface and life of the conventional device . The preparation of the ternary active layer ensures the photoelectric characteristics of the device in the visible-infrared broad spectrum range. In this experiment, the effect of PEIE thickness on the working mode of the device was studied by changing the concentration of the spin-coated PEIE solution. Finally, when the solution of PEIE is less than 0.45wt%, the device works in the diode mode, on the contrary, it works in the photoconductive mode. And under 550nm illumination (optical power 4.02mW/cm2) , the device achieves a responsivity of 1.64A/W and an external quantum efficiency of 370%.
Efficacy of Concurrent Chemoradiotherapy In Subgroups of Stage III Nasopharyngeal Carcinoma: An Analysis Based On 10-Year Follow-Up
Authors: Wang Lei , Zheng Wu , Cheng Wanqin , Xie Dehuan , Lin Feifei , Xia Liangping , Su Yong 
Published: July 2021

Abstract
PurposeTo evaluate the efficacy of concurrent chemoradiotherapy (CCRT) in subgroups of stage III nasopharyngeal carcinoma (NPC) in the context of intensity-modulated radiotherapy (IMRT).Methods272 patients with stage III NPC who underwent IMRT with or without concurrent chemotherapy (CCT) were retrospectively reviewed. Clinicopathological features were evaluated by a Cox regression model to identify independent prognostic factors. Survival outcomes were assessed using Kaplan-Meier method and log-rank test.ResultsThe median follow-up time was 108 months. The 10-year locoregional-free survival (LRFS), distant metastasis-free survival (DMFS), disease-free survival (DFS), and overall survival (OS) rates were 87.8%, 80.7%, 68.8%, and 74.9%, respectively. Multivariate analysis showed that the N classification was significantly associated with DMFS (hazard ratio [HR] 3.616, 95% confidence interval [CI] 1.387-9.428, P=0.009), DFS (HR 2.417, 95% CI 1.291-4.423, P=0.006), and OS (HR 3.024, 95% CI 1.385-6.602, P=0.005). In patients with T1-3N2 disease, CCRT was associated with improved 10-year LRFS (89.6% vs. 65.4%, P=0.005), DFS (71.9% vs. 39.4%, P=0.001) and OS (80.0% vs. 50.5%, P=0.004) compared with IMRT alone. However, in patients with T3N0-1 disease, no significant survival differences were observed between patients treated with IMRT alone and CCRT (P>0.05). ConclusionCCRT is an effective therapy in stage III NPC, especially for patients with N2 disease but N0-1 disease. Individualized treatment strategies are essential for patients with varying disease risks.
Cesarean Delivery on Maternal Request and Common Child Health Outcomes: A Prospective Cohort Study
Authors: Si Ke-yi , Li Hong-tian , Zhou Yu-bo , Li Zhi-wen , Zhang Le , Zhang Ya-li , Ye Rong-wei , Liu Jian-meng 
Published: July 2021

Abstract
Cesarean delivery (CD) versus vaginal delivery was reported to increase the risks of childhood obesity, pneumonia, anemia, and neurobehavioral disorders, but few studies were able to deal with the confounding biases associated with medical conditions indicating cesareans. This prospective cohort study aims to investigate the associations of non-medically indicated CD on maternal request (CDMR) with multiple child health outcomes. Among live-born infants whose mothers participated in a randomized controlled trial on micronutrient supplementation and pregnancy outcomes during 2006-2009 in 5 rural counties in Hebei Province, China, 6972 singletons born by full-term spontaneous vaginal delivery (SVD) and 3626 by CDMR were selected and followed up at 1.5-5 years in 2011. The primary outcome was obesity, defined as a weight-for-height z-score >3. The secondary outcomes included self-reported pneumonia, anemia defined as hemoglobin <110 g/L, and neurobehavioral disorders identified by Child Behavior Checklist and Bayley Scales of Infant Development. Compared with SVD, CDMR was associated with increased risks of obesity (adjusted odds ratio [aOR] 1.41; 95% confidence interval [CI] 1.14-1.75) and anemia (aOR 1.65; 95% CI 1.28-2.12), but not with the risk of pneumonia (aOR 1.16; 95% CI 0.94-1.45) or neurobehavioral disorders (aORs varied from 0.82 to 1.13) in childhood. Conclusion: CD, independent of cesarean indications, is likely associated with childhood obesity and anemia, indicating a need to keep pregnant women informed, especially those seeking CDMR, a need to explore possible improvement on obstetric service, and even a need for main stakeholders to reach a compromise in making a cesarean decision.
A Survey on SDN-based Intrusion Detection Systems on the Internet of Thing: Concepts, Issues, and Blockchain Applications
Authors: Hassan Heba A. , Hemdan Ezz E. , El-Shafai Walid , Shokair Mona , El-Samie Fathi E. Abd 
Published: July 2021

Abstract
With the accelerated development of computer networks utilization and the enormous growth of the number of applications running on top of it, network security becomes more significant. Intrusion Detection Systems (IDS) is considered as one of the essential tools utilized to protect computer networks and information systems. Software-defined network (SDN) architecture is used to provide network monitoring and analysis mechanism due to the programming environment of the SDN controller. On the other hand intrusion detection system is developed to monitor incoming traffic to the SDN network; hence it enables SDN to adjust security service insertion. This paper presents a survey study for SDN with the Internet of Things (IoT) and its improved versions like SDN-based IDS and SDN-based IoT. Likewise, discussing the IoT and its problems, especially the security aspects and solutions to overcome these problems. Finally, a brief description of the Blockchain concept and how it can be merged with an SDN-based IoT system to further enhance its security aspects is provided.
Study of Antimicrobial Susceptibility And Biofilm Formation of Cronobacter Sakazakii Isolates From Neonatal Sepsis In Southwest Iran
Authors: Moradi Melika , dezfuli Aram asareh zdegan , Dargahi Zahra , Amin Mansour , Ahmadkhosravi Nazanin , Motahar Moloudsadat , Jahangirimehr Fatemeh , Meghdadi Hossein , Bakhtiyariniya Pezhman , Heydari Reza , Emam Nasrin , Mehdipour Golshan 
Published: July 2021

Abstract
The authors have requested that this preprint be removed from Research Square.
Association between dietary fatty acids pattern and incidence of oral cancer: A case-control study from Southeast China
Authors: Fan Yi , Qiu Yu , Chen Qing , Wang Sijie , Xu Mingming , Li Yanni , Weng Yanfeng , Qian Jiawen , Wang Jing , Chen Fa , Wang Jing , Shi Bin , Pan Lizhen , Lin Lisong , He Baochang , Liu Fengqiong 
Published: July 2021

Abstract
ObjectiveTo investigate the relationship between dietary fatty acid pattern and the risk of oral cancer (OC).MethodIn 446 patients with primary oral cancer and 448 controls, we assessed prediagnosis consumption of 159 food items by food frequency questionnaires completed within 1 week of diagnosis. Fatty acid patterns were identified using principal component analysis. Odds ratio (OR) and 95% confidence (CI) interval was calculated.ResultsGeneral differences of fatty acid intake were observed between case and control, intake of saturated fatty acids such as C14:0, C16:0, C18:0 are higher in case than control group (p < 0.001), intake of monounsaturated fatty acid such as C18:1 is higher in case than control group (p < 0.001). We identified a fatty acid pattern which was characterized by saturated fatty acids and it explained 33.2% of the overall variability of the 32 fatty acids. The identified fatty acid pattern scores were positively associated with a higher risk of oral cancer [multivariable-adjusted OR comparing extreme quintiles, 3.325 (95%CI: 2.222, 4.975); Ptrend<0.001].ConclusionsGeneral differences of dietary fatty acids were observed between oral cancer patients and controls. A dietary fatty acids pattern, which was mainly composed of saturated fatty acids were positively correlated with the risk of oral cancer.
Diet Content of Non-Saturated Fatty Acids is Related to Lower Plasma Adiponectin in Patients with Metabolic Syndrome
Authors: Suárez-Cuenca Juan Antonio , Díaz-Jiménez Diana Elisa , Pineda-Juárez Juan Antonio , Mendoza-Mota Alondra Gissel , Valencia-Aldana Ofelia Dinora , Núñez-Angeles Said , Vera-Gómez Eduardo , Hernández-Patricio Alejandro , Escamilla-Tilch Mónica , Loeza-Magaña Pavel , Lara-Vargas Jorge Antonio , de Vaca Rebeca Pérez-Cabeza , Arteaga-Martínez José Rodolfo , Garduño-Pérez Ángel Alfonso , Barranco-Fragoso Beatriz , Montoya-Ramírez Jesús , Díaz-Aranda María Angélica , Chaparro-Hernández Roberto Carlos , Melchor-López Alberto , García Silvia , Gutiérrez-Salinas José , Mondragón-Terán Paul 
Published: July 2021

Abstract
Introduction. The metabolic syndrome (MS) has been related to the unbalance between pro- and anti-inflammatory cytokines; where adiponectin, an anti-inflammatory adipokine, is considered to play a key metabolic role. The consumption of certain micronutrients has been claimed to modify pro- and anti-inflammatory cytokines. Aim. To explore whether dietary micronutrients are related with plasma adiponectin in patients with MS. Methods. Cross-sectional analysis. Quartiles of dietary bioactive compounds were compared according to values of plasma adiponectin. Interquartile variation (IQV) and correlation analyses were performed. Results. There was a significant IQV of dietary unsaturated fatty acids (between -50% to -66% of change, p25 vs p75, p<0.05), particularly for trans-fatty acids, poly-unsaturated fatty acids, mono-unsaturated fatty acids and w-6 fatty acids, in relation to plasma adiponectin; as well as a negative correlation (rho= -0.53, -0.37, -0.29 and -0.34, respectively; p<0.05). Conclusion. Dietary amounts of unsaturated fatty acids inversely related to plasma adiponectin in patients with MS.
Ionic Liquid Regenerated Cellulose Membrane Electroless Plated By Silver Layer For ECG Signal Monitoring
Authors: Fu Xueli , Wang Yanping , Wang Wei , Yu Dan 
Published: July 2021

Abstract
Flexible electrodes have attracted the interest of a wide range of people because they can monitor human health signals like ECG, EMG and EEG as wearable devices. However, PDMS-based membrane electrodes have the problem of difficulty in depositing metal layers, while fabric electrodes have high contact impedance. Furthermore, the widely used Ag/AgCl electrodes have the shortcomings of skin inflammation or skin irritation. Therefore, we fabricate a skin-like electrical conductive electrode via electroless silver plating on the surface of regenerated cellulose membrane, in which the cellulose membrane is obtained by the dissolution of cotton fiber with green solvent ionic liquid [Bmim]Cl. The as-prepared biocompatible electrode with low skin-electrode contact impedance can be used as a dry electrode for a long-term period of use. The impedance at 700 Hz is only 8 kΩ/cm2, and the conductivity can reach 252 s/cm. After 5 hours of wear, the skin contact impedance of the electrode was only 10 kΩ/cm2 under 700 Hz(when AgNO3 was used at a concentration of 0.20 mol/L). Importantly, the electrodes not only provide a stable and clear ECG signal, but also offer a high level of comfort and low impedance, when used for long-term health monitoring.
Diagnostic Performance of TE, 2D-SWE And MRE For Liver Fibrosis In Treatment-Naive People With HBV: A Systematic Review And Meta-Analysis
Authors: Li Mingkai , Wan Sizhe , Wu Xiaoying , Wu Bin 
Published: July 2021

Abstract
Background/aims: To assess the performance of transient elastography (TE), two-dimensional shear wave elastography (2D-SWE), and magnetic resonance elastography (MRE) for staging significant fibrosis and cirrhosis in untreated chronic hepatitis B (CHB) patients. Methods: Pubmed, Embase, Web of Science and Cochrane Library were searched for terms involving CHB, TE, SWE, and MRE. Other etiologies of chronic liver disease (CLD), previous treatment in patients or articles not published in SCI journals were excluded. Hierarchical non-linear models were used to evaluate the diagnostic accuracy of TE, 2D-SWE and MRE. Heterogeneity was explored via analysis of threshold effect and meta-regression. Results: Twenty-eight articles with a total of 4540 untreated CHB patients were included. The summary AUROC using TE, 2D-SWE and MRE for predicting significant fibrosis (SF) were 0.84, 0.89, and 0.99, respectively. MRE is more accurate than both TE (P＜0.01) and 2D-SWE (P＜0.01) in staging SF. 2D-SWE is superior to TE in detecting SF (P＜0.01). The summary AUROC employing TE, 2D-SWE and MRE for detecting cirrhosis were 0.9, 0.94, and 0.99, respectively. TE displayed a similar diagnostic accuracy with 2D-SWE in staging cirrhosis (P=0.14). MRE and 2D-SWE are comparable for staging cirrhosis (P=0.08). MRE is superior than TE (P＜0.01) in staging cirrhosis.Conclusion: TE, 2D-SWE, and MRE express acceptable diagnostic accuracies in staging staging significant fibrosis and cirrhosis in untreated CHB patients. Both MRE and 2D-SWE are better choices while the TE can be regarded as a secondary option.
Quantitative Microbial Population Study Reveals Geographical Differences In Bacterial Symbionts of Ixodes Ricinus
Authors: Krawczyk Aleksandra I. , Röttjers Lisa , Fonville Manoj , Takumi Katsuhisa , Takken Willem , Faust Karoline , Sprong Hein 
Published: July 2021

Abstract
Background: Ixodes ricinus ticks vector pathogens that cause serious health concerns. Like in other arthropods, the microbiome may affect the tick’s biology with consequences for pathogen transmission. Here, we explored the bacterial communities of I. ricinus across its developmental stages and six geographic locations by the 16S rRNA amplicon sequencing, combined with quantification of the bacterial load. Results: A wide range of bacterial loads was found. Accurate quantification of low microbial biomass samples permitted comparisons to high biomass samples, despite the presence of contaminating DNA. The bacterial communities of ticks were associated with geographical location rather than life stage, and differences in Rickettsia abundance determined this association. Subsequently, we explored the geographical distribution of four vertically-transmitted symbionts identified in the microbiome analysis. For that, we screened 16,555 nymphs from 19 forest sites for Rickettsia helvetica, Rickettsiella spp., Midichloria mitochondrii, and Spiroplasma ixodetis. The infection rates of all vertically-transmitted symbionts differed between the study sites, and none of them was present in all tested ticks, suggesting facultative associations with I. ricinus. The proportions in which symbionts occurred in populations of I. ricinus were highly variable, but geographically close study sites expressed similar proportions. These patterns were in contrast to what we observed for the horizontally-transmitted pathogens Borrelia burgdorferi sensu lato, Anaplasma phagocytophilum, and Neoehrlichia mikurensis. Lastly, nearly 12% of tested nymphs were free of any targeted microorganisms, which is in line with the microbiome analyses.Conclusions: Our results show that the microbiome of I. ricinus is highly variable, but changes gradually and ticks originating from geographically close forest sites express similar bacterial communities. This suggests that geography-related factors affect the infection rates of vertically-transmitted symbionts in I. ricinus. Since some symbionts, such as R. helvetica can cause disease in humans, we propose that public health investigations consider geographical differences in its infection rates.