bluuebunny/crossref_metadata_2025_split huggingface dataset:
Dirofilaria immitisandD. repensin Europe: a systematic literature review on vectors, host range, and the spatial distribution in the 20th and 21st century
Authors: Hattendorf Carolin , Lühken Renke 
Published: February 2025

AbstractBackgroundDirofilaria immitisandD. repensare mosquito-borne nematodes with dogs as primary hosts, but other mammalian species including humans can be also infected. In the last century, circulation of both pathogens was predominantly restricted to Southern Europe. However, different studies indicated a potential establishment in Central, Eastern and Western parts of Europe as an increasing threat to animal and human health.MethodsWe conducted a systematic literature review of publications reportingD. immitisandD. repensscreening in mosquitoes and mammalian vertebrates in Europe. These data were used to analyse the range of vectors and hosts and for a comparison of the spatial distribution between the 20thand 21stcentury.ResultsBoth nematodes appear to have a high overlap ofAedes,AnophelesandCulexvector species, which are abundant in Europe. MostD. immitisinfections were reported in dogs, whileD. repenspredominated in humans.Dirofilaria immitisinfections were detected in a wider range of wild and zoo animals. Compared to the last century, many more countries especially in Central Europe were affected byDirofilariaspp. circulation, illustrating a significant spread over the last 20 years.ConclusionOur findings suggest thatD. immitisandD. repensare a growing health concern for animals and humans in Europe. Continuous globalisation and climate warming will probably lead to a further spread and increased circulation in the future. All data are made available open access, which will enable further analysis in the future.
SUGP1 loss is the sole driver of SF3B1 hotspot mutant missplicing in cancer
Authors: Xing Peiqi , Bak-Gordon Pedro , Xie Jindou , Zhang Jian , Liu Zhaoqi , Manley James L. 
Published: February 2025

AbstractSF3B1 is the most frequently mutated splicing factor in cancer. Mechanistically, such mutations cause missplicing by promoting aberrant 3’ splice site usage; however, how this occurs remains controversial. To address this issue, we employed a computational screen of 600 splicing-related proteins to identify those whose reduced expression recapitulated mutant SF3B1 splicing dysregulation. Strikingly, our analysis revealed only two proteins whose loss reproduced this effect. Extending our previous findings, loss of the G-patch protein SUGP1 recapitulated almost all splicing defects induced bySF3B1hotspot mutations. Unexpectedly, loss of the RNA helicase Aquarius (AQR) reproduced ∼40% of these defects. However, we found that AQR knockdown caused significantSUGP1missplicing and reduced protein levels, suggesting that AQR loss reproduced mutant SF3B1 splicing defects only indirectly. This study advances our understanding of missplicing caused by oncogenicSF3B1mutations, and highlights the fundamental role of SUGP1 in this process.
OTX2 controls chromatin accessibility to direct somatic versus germline differentiation
Authors: Barbieri Elisa , Chambers Ian 
Published: February 2025

SummaryThe choice between somatic and germline fates is essential for species survival. This choice occurs in embryonic epiblast cells, as these cells are competent for both somatic and germline differentiation. The transcription factor OTX2 regulates the choice between somatic and germline fates, asOtx2-null epiblast-like cells (EpiLCs) form primordial germ cell-like cells (PGCLCs) with enhanced efficiency. However, the mechanisms by which OTX2 achieves this function are not fully characterized. Here we show that OTX2 controls chromatin accessibility at specific chromatin loci to modulate gene transcription and enable somatic differentiation. By performing CUT&RUN for OTX2 and ATAC-seq in wild-type andOtx2-null embryonic stem cells and EpiLCs, we identified regions where OTX2 binding opens chromatin, either alone or in cooperation with a ZIC family protein. Enforced OTX2 expression maintains accessibility at these regions and, in addition, induces opening of ∼4,000 somatic-associated regions in cells differentiating in the presence of PGC-inducing cytokines. Once cells have acquired germline identity, these additional somatic-associated regions can no longer respond to OTX2 and remain closed. Our results indicate that OTX2 works in cells with dual competence for both somatic and germline differentiation to increase accessibility of somatic regulatory regions and induce the somatic fate at the expense of the germline.
Decision-making insights on the prepositioning of relief items: a systematic review
Authors: Shariati Amir , L’Hermitte Cécile , Trent Nadia Millis 
Published: February 2025

Purpose
This study aims to review the prepositioning of relief items literature through a decision-making lens to explore the decisions involved, the factors guiding them and the influence of model design on these decisions. Despite their potential to inform decision-making, quantitative prepositioning models remain underutilised in practice. Understanding the foundational principles that shape model design and their connections to decision-making is crucial for effectively developing and implementing more practical models.

Design/methodology/approach
A systematic literature review was conducted, and 97 relevant papers were analysed bibliographically and thematically. The thematic analysis is guided by the value-focused thinking approach, which provides a structured understanding of the decision-making process by focusing on the decision makers’ values.

Findings
This study identifies key prepositioning decisions related to facilities, inventory and distribution. It highlights efficiency, effectiveness and equity as the main values guiding prepositioning decisions and examines the mutual influence of model design and decisions. Moreover, a decision-making framework for prepositioning problems has been developed, outlining key steps and relevant decisions.

Originality/value
This research provides novel insights into how the decision-making process regarding prepositioning is reflected in quantitative models. It helps researchers choose model designs that better align with decision makers’ priorities and requirements, increasing the models’ practicality. Additionally, it helps decision makers comprehend quantitative models and the reasons behind their mathematical complexities, ultimately improving the effectiveness of decision-making for prepositioning.
Board gender diversity and corporate environmental, social and governance performance: evidence from New Zealand listed firms
Authors: Zharfpeykan Ramona , Bai Yuanzhen 
Published: February 2025

Purpose
This study aims to examine whether board gender diversity is significantly and positively associated with corporate environmental, social and governance (ESG) performance, as well as the link between board gender diversity and each of the three ESG pillars.

Design/methodology/approach
Aligning with stakeholder and critical mass theories, this study uses ordinary least squares regression and fixed-effects models on the sample comprising New Zealand listed firms from 2016 to 2022.

Findings
The findings reveal that board gender diversity positively and significantly is associated with firms’ overall ESG performance. In addition, female board representation positively and significantly associates with the governance pillar but, not with the other two. The findings highlight that a higher proportion of female board representation enhances a firm’s overall ESG performance by improving its corporate governance initiative.

Originality/value
Besides adding to the literature on board gender diversity’s association with New Zealand listed firms’ ESG performance, the findings provide insights for companies, policymakers and stakeholders.
Affordable 3D printing methodology for the fabrication of orthotic insoles aimed at reducing plantar pressure for diabetic patients
Authors: Koprivnik Mathieu , A. Marquette Christophe , Courtial Edwin-Joffrey 
Published: February 2025

Purpose
This study aims to address the pressing need for innovative, accessible and cost-effective healthcare solutions for diabetic foot complications by exploring additive manufacturing, specifically fused deposition modelling (FDM), to create patient-specific orthotic insoles.

Design/methodology/approach
The research aims to streamline design and production processes, reducing lead times and costs associated with traditional orthotic manufacturing methods. The study involves a detailed examination of FDM technology to produce custom orthotic insoles, focusing on mechanical evaluations of various candidate materials based on their viscoelastic properties. Different infill lattice patterns, such as Honeycomb and Gyroid, are assessed to determine their effectiveness in mitigating plantar pressures.

Findings
The research outlines a comprehensive workflow for the FDM printing process, highlighting the significance of tailored infill percentages to achieve the desired stiffness levels in the insoles. Filaflex 60A is identified as a suitable material for FDM printing of orthotic insoles due to its favourable viscoelastic properties, comparable to established orthotic materials. Among the tested infill lattice patterns, Gyroid structures demonstrate superior resilience under compression, making them more effective in reducing plantar pressures. Despite challenges such as soft filament handling and printing speed limitations, FDM printing is shown to be a viable alternative to traditional manual techniques, offering benefits in automation and cost-effectiveness.

Originality/value
This research presents a novel approach to orthotic insole production using FDM technology, significantly contributing to diabetic foot care. By providing a comprehensive workflow and emphasizing the importance of material selection and infill design, the study paves the way for further advancements in patient-specific healthcare solutions through innovative and affordable manufacturing techniques.
Implications of summer breeding phenology on demography of monarch butterflies
Authors: Debinski Diane M. , Warchola Norah , Altizer Sonia , Crone Elizabeth E. 
Published: February 2025

Abstract

Phenological changes have been widely documented in animal and plant responses to directional environmental change. However, predicting the consequences of these shifts for species interactions and population viability requires knowledge of vital rate responses to biotic and abiotic drivers.

Here, we paired long‐term phenology data documenting monarch butterfly abundance and occurrence of their milkweed hostplant with outdoor experiments in the central United States to ask how changes in spring arrival times to monarch breeding sites affect their development, survival, and within‐season population growth.

Monarch arrival times did not change across the 17 years of monitoring, but the peak abundance of monarchs, which occurred just prior to their fall migration, shifted 9 days later in 2019 as compared to 2003. Summer population growth declined from 2003 to 2019, significant in ~80% bootstrap calculations. Phenological changes in milkweed occurrence mirrored changes in monarch abundance, happening later through time. Our field experiment showed that early season larval survival was highest when the timing of hatching matched the average timing of the first natural monarch cohort; survival was lowest when egg hatching shifted 14 days earlier.

The results of our study indicate that earlier arrival of adult monarchs to summer breeding habitat would be costly for monarchs—but field survey data show that arrival times have not changed to date. Instead, the local changes we observed in the timing of peak abundance occurred towards the end of the breeding season, not the onset. At present, we conclude that changes in early season phenology are not a threat to eastern North American monarchs living in the central United States, but drivers of breeding‐season growth rates and changes in late‐season phenology merit further study, both in the central United States and in other parts of the monarch's range.
Microbial necromass carbon drives soil organic carbon accumulation during long‐term vegetation succession
Authors: Zhao Ziwen , Qin Yanli , Wu Yang , Chen Wenjing , Wang Hao , Chen Jiawen , Yang Jinqiu , Liu Guobin , Xue Sha 
Published: February 2025

Abstract

Plant‐ and microbial‐derived carbon (C) are the two major sources of soil organic carbon (SOC) pools that make important contributions to stable and labile SOC. Although the hypothesis of an increase in SOC during natural vegetation restoration has been broadly verified, the contributions of plant‐ and microbial‐derived C to SOC accumulation remain uncertain.

In this study, we used biomarker approaches to assess the contribution and allocation of plant‐ and microbial‐derived C in long‐term vegetation succession sequences.

We found a unimodal distribution of total lignin phenols along vegetation succession, with the maximum occurring at 100 years of succession (293 ± 22.7 mg kg−1). Vegetation succession significantly increased microbial‐derived C, including microbial necromass C (MNC) and glomalin‐related soil proteins (GRSP). The contribution of MNC to SOC was high (26%–49%) and increased significantly with vegetation succession, whereas the proportion of plant‐derived C and GRSP in SOC consistently decreased.

The results indicated that the distribution of lignin phenols is determined by the quality and abundance of plant litter input to the soil, and the increase in microbial‐derived C is closely associated with microbial metabolism mediated by environmental factors. However, the C turnover pathway from microbial necromass to persistent SOC formation, as inferred from the nonlinear response of the MNC accumulation coefficient, requires 90–100 years to achieve a stable contribution to soil C sequestration.

Synthesis and applications. Our findings further highlight the critical role of the microbial C pump in SOC formation and accumulation. We argue that prioritizing native pioneer species and their mixed communities with climax species during revegetation of extensive fragile ecosystems contributes to sustainable soil C sequestration practices.
Parent and adolescent outcomes from a family‐based treatment‐informed day programme for adolescents with restrictive eating disorders: A pilot study
Authors: Wilson Daniel , Loxton Natalie , Middeldorp Christel , Geissler Ingrid , Catania Salvatore , Withington Tania 
Published: February 2025

AbstractDay programmes designed for young people with restrictive eating disorders offer intensive treatment options for individuals who have not made satisfactory progress through outpatient treatment but do not require hospitalisation. Existing literature consistently shows positive outcomes in terms of weight gain, reduced eating disorder and comorbid symptomology. However, it is crucial to note that although many day programmes consider family‐based treatment a core framework, research on treatment outcomes for parents and the relationship between parent outcomes and adolescent outcomes remains limited. The current pilot study evaluated parent (n = 20) and adolescent (n = 13) outcomes of a new day programme for adolescents with restrictive eating disorders and their families. Results showed significant improvements across body mass index, eating disorder and comorbid symptomatology for adolescents and improvements in parent self‐efficacy, parent mental health and a reduction in eating disorder accommodating behaviours. The findings suggest several potential mediators of day programme outcomes that could be tested in future research.
Jung’s Koan of the Self and a Zen Buddhist Solution
Authors: Anderson Daniel 
Published: February 2025

Abstract“God is a circle, whose centre is everywhere, whose circumference is nowhere.” Jung repeats this ancient saying often when describing the Self. This article frames the saying as a type of koan and uses Zen Buddhism’s conception of the Buddha‐nature to unlock the psychological meaning of Jung’s saying and to suggest new perspectives on Jung’s notion of the Self. Parallels between Jung’s notion of the Self and Zen’s Buddha‐nature emerge with particular clarity in Mysterium Coniunctionis in connection with Jung’s discussion of the alchemical unus mundus and caelum. The essay further delves into the relationship between Zen teachings on enlightenment and Jung’s notion of individuation and explores how these insights can inform Jungian theory and practice. By addressing the implications of Zen concepts for understanding the Self, the essay ultimately seeks to illuminate the shared spiritual dimensions of Jungian psychology and Zen Buddhism and to provide new perspectives on both.
Identifying bromazolam, etizolam, and flualprazolam in blood using gas chromatography–mass spectrometry
Authors: Ballotari Marco , Truver Michael T. , Baer Devin C. , Brogan Sara C. , Hoyer Jennifer L. , Crosby Michele M. , Goldberger Bruce A. , Chronister Chris W. 
Published: February 2025

AbstractDesigner benzodiazepines are a commonly misused class of novel psychoactive substances (NPS) that present challenges for forensic toxicology laboratories and public health. Due to the difficulty in detection by routine toxicology methods, it is important to enhance the analytical methods for the identification and quantitation of designer benzodiazepines. The aim of this study was to identify and quantify designer benzodiazepines using gas chromatography coupled with mass spectrometry (GC‐MS) in postmortem blood specimens. A method originally validated to identify and quantitate alprazolam was modified to quantitate flualprazolam, etizolam, and bromazolam, as well as identify adinazolam and 4′‐chloro‐deschloroalprazolam. The method was subsequently used to analyze postmortem specimens (n = 35) from Medical Examiner Districts (n = 4) in Florida that screened positive for one or more designer benzodiazepines from February 2022 to December 2023. Among the screened samples, 21 tested positive for one or more designer benzodiazepines in the postmortem blood; 1 was positive for etizolam (90 ng/mL) and flualprazolam (<5.0 ng/mL), while 20 were positive for bromazolam. Bromazolam was the most frequently detected designer benzodiazepine, with a concentration range of 5.9–352 ng/mL (mean: 59.1 ng/mL; median: 28.5 ng/mL) for 18 decedents and <5.0 ng/mL for two decedents. Polydrug use was confirmed in all decedents. The most prevalent drugs found with bromazolam in the blood were fentanyl, methamphetamine and/or amphetamine, and cocaine. The optimized GC‐MS method provides a way to identify and quantitate designer benzodiazepines in postmortem blood and to also qualitatively monitor two newer designer benzodiazepines: 4′‐chloro‐deschloroalprazolam and adinazolam.
MESR4 targets bam to mediate intestinal homeostasis and aging in adult flies
Authors: Cai Qingshuang , Wang Zixuan , Xiao Yihua , Zhang Chuchu , Yang Yulong , Kong Fanrui , Feng Yue , Guo Huimin , Saeed Muhammad Abdul Rehman , Ali Umar , Li Weini , Ji Shanming 
Published: February 2025

AbstractThe Drosophila melanogaster (fruit fly) misexpression suppressor of Ras 4 (MESR4) gene encodes a potential transcription factor and plays critical roles in various biological processes, including embryonic development, lipid metabolism, eye–antennal development, and germline stem cell differentiation. However, whether it is involved in modulating intestinal homeostasis remains elusive. In this study, we provide compelling evidence demonstrating that MESR4 is a bona fide regulator in preventing age‐onset intestinal leakage and dysbiosis in adult flies. Mechanistically, MESR4 is predominantly located at the nucleus of intestinal cells and controls the expression of bag‐of‐marbles (bam), thereby restricting the excessive activation of immune deficiency signaling during aging. The silencing of Relish (Rel), which encodes a key transcription factor of the immune deficiency signaling pathway, reverses the beneficial effects of MESR4 in mediating intestinal barrier function and fly lifespan. Collectively, our studies uncover an undescribed function of Drosophila MESR4 in the maintenance of intestinal homeostasis and overall organismal fitness.
Preventive efficacy of sprouting black soybean peptides on high‐salt diet‐induced hypertension in mice
Authors: Cheng Dawei , Huang Xinyu , Shao Ben , Zhang Caihong , Li Xueling , Li Meiqing 
Published: February 2025

AbstractThis study examined possible mechanisms of action as well as the preventive and interventional effects of sprouting black soybean peptides (SBSPs) and black soybean peptides (BSPs) on hypertension in C57BL/6j mice that was brought on by a high‐salt diet. BSP and SBSP were administered to mice in the black soybean peptide prevention (BSP‐P) group and sprouting black soybean peptide prevention (SBSP‐P) group starting 4 weeks prior to the high‐salt diet, respectively. Mice in the black soybean peptide intervention (BSP‐I) group and the sprouting black soybean peptide intervention (SBSP‐I) group received oral doses of BSP and SBSP, respectively, together with a high‐salt diet. The findings demonstrated that BSP‐I, BSP‐P, SBSP‐I, and SBSP‐P prevented the activation of localized angiotensin converting enzyme (ACE)/angiotensin II (Ang II) pathways in the kidneys and circulation, delayed the rise in blood pressure in mice, and preserved the functional nitric oxide/endothelin‐1 (NO/ET‐1) balance of endothelium. The inflammatory factors tumor necrosis factor‐α (TNF‐α), interferon‐γ (IFN‐γ), and interleukin‐6 (IL‐6) had decreased plasma levels. Additionally, it improved fibrosis, renal edema phenomena, and cardiomegaly. Furthermore, the expression of genes related to the renin–angiotensin system and endothelial function is regulated by SBSP and BSP. On the other hand, compared to intervention effects, SBSP and BSP showed more noticeable preventive benefits on hypertension brought on by a high‐salt diet. When all else was equal, SBSP was more effective than BSP at avoiding hypertension. As a result, this study offers theoretical backing for SBSP as a dietary supplement source for the prevention of high‐salt diet‐induced hypertension.
Rhamnogalacturonan‐I domains from red dragon fruit pectin promote the proliferation of Bifidobacterium animalis
Authors: Zhang Meng‐yuan , Dong Lei , Wen Yin , Deng Jie‐qi , Cai Jun 
Published: February 2025

AbstractPectin‐derived oligosaccharides, an important source of the new generation of prebiotics, have aroused widespread research. As per our previous study report, the rhamnogalacturonan‐I (RG‐I) type pectin of dragon fruit positively affects the proliferation of Bifidobacterium animalis subsp. Lactis 3296. However, the pectin region that primarily produces prebiotic effects is not clear. To further investigate the promotion of the dragon fruit pectin RG‐I domain on the proliferation of B. animalis subsp. Lactis 3296, we first obtained pectin (DP‐AC‐A2a) with a higher proportion of the RG‐I region through gel purification and enzyme digestion. Then, monosaccharide components and Fourier transform infrared were analyzed to identify the pectin structures preliminarily. The results demonstrated that DP‐AC‐A2a with a backbone consisting of repeated → 4 GalpA 1–2 Rhap 1 → and the branches are mainly composed of 1,4‐linked galactose. Furthermore, the bacterial proliferation experiment proved the contribution of galactan on the pectin's branch to the proliferation of B. animalis. The enzyme activity showed that the addition of DP‐AC‐A2a can promote the production of β‐d‐galactosidase by B. animalis. These data provide effective information for the potential application of red dragon fruit pectin.