bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effect of CD146 on Bone Regeneration by Transplantation of Stem Cells from Human Exfoliated Deciduous Teeth into Mouse Skull Defect
Authors: Rikitake Kodai , Kunimatsu Ryo , Yoshimi Yuki , Nakajima Kengo , Hiraki Tomoka , Putranti Nurul Aisyah Rizky , Tsuka Yuji , Abe Takaharu , Ando Kazuyo , Hayashi Yoko , Nikawa Hiroki , Tanimoto Kotaro 
Published: April 2021

Abstract
Stem cells from human exfoliated deciduous teeth (SHED) possess bone regeneration ability and may have therapeutic applications. CD146, a cell adhesion protein expressed by vascular endothelial cells, is involved in the osteoblastic differentiation of stem cells. However, the effect of CD146 on SHED-mediated bone regeneration in vivo remains unknown. Hence, in this study we aimed to establish efficient conditions for SHED transplantation. SHED were isolated from the pulp of an extracted deciduous tooth and cultured, and CD146-positive (CD146+) and CD146-negative (CD146−) populations were sorted. Heterogeneous populations of SHED and CD146+ and CD146– cells were transplanted into bone defects generated in the skulls of individual immunodeficient mice. Micro-computed tomography was performed immediately post-transplantation and at 4- and 8-weeks thereafter to evaluate bone regeneration. Histological and immunohistochemical assessments were also performed at 8 weeks after transplantation. Micro-computed tomography revealed bone regeneration upon transplantation with CD146+ and heterogeneous populations of SHED, particularly at 8 weeks after transplantation, with significantly higher bone regeneration observed following transplantation with CD146+ cells. Furthermore, histological and immunohistochemical assessments revealed that CD146+ cells promoted bone regeneration and angiogenesis. Therefore, transplantation of CD146+ SHED into bone defects may serve as a useful strategy for bone regeneration.
Long-Term Outcomes of Fully Covered Self-Expandable Metal Stents Versus Plastic Stents in Chronic Pancreatitis
Authors: Lee Sang Hoon , Kim Yeon Suk , Kim Eui Joo , Lee Hee Seung , Park Jeong Youp , Park Seung Woo , Song Si Young , Cho Jae Hee , Bang Seungmin 
Published: April 2021

Abstract
Chronic pancreatitis (CP) related main pancreatic duct (MPD) stricture has been a challenge for endoscopists. Fully covered self-expandable metal stents (FC-SEMS) has been tried in CP patients, but the efficacy and safety are still controversial. Thus, we aim to compare the long-term clinical efficacy of FC-SEMS vs. plastic stent placement in persistent MPD strictures secondary to CP. Between 2007 and 2018, 80 chronic pancreatitis patients (58 males, median age 49 years), who underwent endoscopic placement of FC-SEMS (n=26) and plastic stent (n=54) for persistent MPD strictures, were retrospectively analyzed during a median follow-up duration of 33.7 months. As a result, MPD stricture resolution rate was statistically higher in FC-SEMS group (87.0% vs. 42.0%, p < 0.001). Although immediate complications occurred similarly (38.5% vs. 37.0%, p = 0.902), spontaneous migration (26.9%) and de novo strictures (23.1%) were pronounced delayed complications in FC-SEMS group. Pain relief during follow-up was significantly higher in FC-SEMS group (76.9% vs. 53.7%, p = 0.046). The total procedure cost was similar in both groups ($1,455.6 vs. $1,596.9, p = 0.486). In comparison with plastic stent, FC-SEMS placement for persistent MPD strictures had favorable long-term clinical efficacy, with its typical complications like spontaneous migration and de novo strictures.
The Complete Mitochondrial Genome Sequence Variation and Phylogenetic Analysis of Mulberry
Authors: Liangliang Guo , Yisu Shi , Mengmeng Wu , Ackah Michael , Peng Guo , Danyan Zheng , Lin Qiang , Zhao Weiguo 
Published: April 2021

Abstract
Mulberry is admired for its landscaping and possesses high development prospects and scientific research value. Mitochondria are the plants' powerhouse that produces energy to carry out life processes. In this study, the mt genome of Morus L(M. atropurpurea and M. multicaulis)were annotated and assembled. The circular mt genome of M. multicaulis has a length of 361,546bp, contains 54 genes, including 31 protein-coding genes, 20 tRNA genes, and 3 rRNA genes and composition of A(27.38%), T (27.20%), C (22.63%) and G (22.79%). The sequence repeats, RNA editing gene and migration from cp to mt and was observed in M. multicaulis mt genome. Phylogenetic analysis based on the complete mt genomes of Morus and other 28 species reflects an exact evolutionary and taxonomic status. Furthermore, we investigation on mt genome size, organization, and plastomes at the global level and pi analysis of Morus genome was investigated and compared to other land plants. The results indicate that the exist mt genome's variation in plants. We reported the mt genome assembly and annotation of a halophytic model plant, M. multicaulis, and subsequent analysis, which provided us with a comprehensive understanding of the Morus mt genome.
Hazardous Alcohol Drinking and Associated Factors Among Patients with Tuberculosis Attending Public Healthcare Facilities in Gedeo Zone, Southern Ethiopia
Authors: Yohannes Kalkidan , Ayano Getinet , Koirita Kusse , Mulatu Henok , Mokona Hirbaye 
Published: April 2021

Abstract
Background: Hazardous drinking is a quantity or pattern of alcohol consumption that places individuals at risk for adverse health events. Studies have shown that hazardous alcohol drinking among patients with tuberculosis has been often linked to a decrease in immune system functioning, greater rates of treatment-resistant cases, and death during treatment. However, there are rare studies that assessed the prevalence of hazardous alcohol use and associated factors among patients with tuberculosis in Ethiopia. Therefore, this study was aimed to assess the prevalence of hazardous alcohol drinking and associated factors among patients with tuberculosis attending public healthcare facilities in Gedeo Zone, Southern Ethiopia.Methods: An institution-based, cross-sectional study was conducted among a randomly selected sample of 415 patients with tuberculosis. The study was conducted at the tuberculosis unit of public healthcare facilities in the Gedeo zone, Southern Ethiopia. The Alcohol Use Identification Test (AUDIT) scale was used to measure hazardous alcohol drinking. Data on social support, perceived tuberculosis stigma, and depression were collected using a standard, validated, and structured questionnaire. Results: The prevalence of hazardous alcohol drinking in our study was 20 % (95% CI; 16.1% -24.2%). Medical comorbidity (AOR = 2.44, 95% CI: 1.29–4.62), length of illness (>=12 months) (AOR = 2.88, 95% CI: 1.03–3.04), and being male (AOR = 2.10, 95% CI: 1.17–3.77) were found to be significant predictors of hazardous alcohol drinking after controlling for potential confounders.Conclusion: A high prevalence of hazardous alcohol drinking was observed among patients with tuberculosis. Therefore, routine screening for alcohol use and a holistic treatment approach for patients with tuberculosis are crucial.
DksA is a central regulatory switch for stress protection and virulence in Acinetobacter baumannii
Authors: Maharjan Ram , Sulivan Geraldine , Adams Felise , Delgado Natasha , Semenec Lucie , Dinh Hue , Li Liping , Short Francesca , Parkhill Julian , Paulsen Ian , Barquist Lars , Eijkelkamp Bart , Cain Amy 
Published: April 2021

Abstract
Bacterial coordination of stress resistance mechanisms in harsh environments is key to long-term survival and evolutionary success. In many Gram-negative pathogens, both general- and specific-stress response are controlled by alternative sigma factors such as RpoS. The critically important pathogen Acinetobacter baumannii is notoriously recalcitrant to external stressors, yet it lacks RpoS, so the molecular control of its resilience remains unclear. Here, we used transposon insertion sequencing to characterize the molecular responses of Acinetobacter baumannii to two biologically-important metals stressors, zinc and copper, and discovered that the transcriptional regulator DksA acts as a major regulatory stress-protection switch. We mapped the highly pleiotropic nature of DksA using transcriptomics and phenomics and found that it controls ribosomal protein expression, metabolism of gluconeogenic substrates and survival in stresses that cause oxidative damage. A. baumannii strains lacking DksA were no longer virulent in both murine and Galleria mellonella in vivo models. In vitro, DksA mutants exhibited increased sensitivity to human serum and antibiotics yet promoted biofilm and capsule formation. Our study provides detailed insight into the unique role that DksA plays in stress protection and virulence for A. baumannii and lays the groundwork for understanding of RpoS-independent regulatory general stress response.
Self-Compassion in Medical Students: A Pilot Study of Its Association With Professionalism Pressure
Authors: Světlák Miroslav , Daňhelová Šárka , Kóša Barbora , Slezáčková Alena , Šumec Rastislav 
Published: April 2021

Abstract
Background: To be a “good doctor” and have “good medical practices” are apparent goals for both medical students and medical faculties. However, the associated implicit and explicit standards could be a source of distress in the form of pressure to achieve professionalism. Self-compassion has been identified as a transtherapeutic factor that plays a crucial role in developing and maintaining mental health. It seems to be an essential meta-skill to learn, especially for medical students who often perceive imperfection as failure. In this pilot study, we investigated the qualities that medical students attribute to the “good doctor” concept, how they perceive themselves compared to this concept, and whether any possible discrepancy between these two perspectives could be associated with self-compassion. Methods: Altogether, 301 medical students participated in the study (mean age 22.3 ± 2.1; 71.8% female). The discrepancy between concepts was measured by a semantic differential consisting of a list of 36 adjectives and antonyms that students repeatedly mentioned in courses in their responses to the question “What should a doctor be like?” Self-compassion was measured by the Self-Compassion Scale. Results: The obtained results offer an insight into students’ conceptualization of a “good doctor” and the hierarchy of given characteristics. Statistical analysis revealed significant associations between the discrepancy between the “ideal” doctor concept vs. actual self-perception and Self-Compassion Scale scores. The more students are compassionate to themselves, the lower the discrepancy.Conclusions: The current pilot study supports the hypothesis that student self-compassion plays some role in the degree of discrepancy between the ideal “good doctor” image and student self-concept. This result could support the importance of educational interventions developing self-compassion for medical students. The proposed discrepancy measurement could also be a tool for measuring the effect of well-being programs aimed at self-compassion in medical students.
Phosphodiesterase 10A is a critical target for neuroprotection in a mouse model of ischemic stroke
Authors: Beker Mustafa C. , B.Caglayan Ahmet , Altunay Serdar , Ozbay Elif , Ates Nilay , Kelestemur Taha , Caglayan Berrak , Kilic Ulkan , Doeppner Thorsten R. , Hermann Dirk M. , Kilic Ertugrul 
Published: April 2021

Abstract
Phosphodiesterase 10A (PDE10A) hydrolyzes adenosine 3′,5′-cyclic monophosphate (cAMP) and guanosine 3′,5′-cyclic monophosphate (cGMP). It is highly expressed in the striatum. Recent evidence implied that PDE10A may be involved in the inflammatory processes following injury, such as ischemic stroke. Its role in ischemic injury was unknown. Herein, we exposed mice to 90 or 30 min middle cerebral artery occlusion, followed by the delivery of the highly selective PDE10A inhibitor TAK-063 (0.3 mg/kg or 3 mg/kg) immediately after reperfusion. Animals were sacrificed after 24 or 72 hours, respectively. Both TAK-063 doses enhanced neurological function, reduced infarct volume, increased neuronal survival, reduced brain edema, and increased blood-brain barrier integrity, alongside cerebral microcirculation improvements. Post-ischemic neuroprotection was associated with increased phosphorylation (i.e., activation) of pro-survival Akt, Erk-1/2 and GSK-3α/β, decreased phosphorylation (i.e., activation) of pro-survival mTOR, increased HIF-1α, MMP-9 and anti-apoptotic Bcl-xL abundance, and reduced pro-apoptotic Bax abundance. Interestingly, PDE10A inhibition reduced inflammatory cytokines/chemokines, including IFN-γ and TNF-α, analyzed by planar surface immunoassay. In addition, liquid chromatography-tandem mass spectrometry revealed 40 proteins were significantly altered by TAK-063. Our study established PDE10A as a target for ischemic stroke therapy.
Investigation of Difference Between the Three Representatives of the Functional Status According to Executive Functions and Participation in Children With Hemiplegic Cerebral Palsy
Authors: Bingöl Hasan , Günel Mintaze Kerem , Sel Sinem asena 
Published: April 2021

Abstract
Aim: This study aimed to explore whether the three functional status representatives differentiate according to actual performance in everyday life. Method: High to moderate functional motor and communication performance levels as described by Manual Ability Classification System (MACS), Gross Motor Function Classification System, and Communication Function Classification System (CFCS) were investigated in the context of the scaled scores of executive function tasks and participation in different life situations on ABILHAND-Kids and Child and Adolescent Scale of Participation (CASP) questionnaires. The data were collected from 98 children with hemiplegic cerebral palsy (CP) mainstreamed in regular schools (mean age 9,3 years [SD 3.0 years], [%95 CI:8.7-9.9]; 42 females, 56 males); 28,6% classified to level I, 29,6 % to level II, and 41,8 % to level III in MACS. Results: The study findings demonstrated that scaled scores of the executive function tasks and participation in different life situations increased with the greater MACS, GMFCS, and CFCS levels (p<0,5).Conclusion: Data from both MACS, GMFCS and CFCS might be useful to predict a child’s actual performance in daily life.
Assessment of quality and safety of pork treated with low-temperature atmospheric-pressure plasma
Authors: Moskalenko N. Yu. , Kudryashova O. A. , Kudryashov L. S. , Tikhonov S. L. , Tikhonova N. V. , Pestov V. V. 
Published: April 2021

It is known that processing methods ensuring partial or full microbial inactivation are quite limited. Therefore, it is of great interest to develop technique and technologies allowing the effective action on microorganisms without a significant influence on product properties. The use of cold plasma can be one of the promising methods of meat product treatment by cold sterilization. The present work examines a possibility of chilled meat treatment with low-temperature atmospheric-pressure plasma to increase its stability to microbial spoilage and extend shelf life. To obtain low temperature plasma, the equipment developed by the designing department “Plasmamed” was used. Chilled meat was treated with low-temperature atmospheric-pressure argon plasma for 5, 10, 20 and 30 min. Samples were stored at a temperature of 2–4 °C for 10 days. Organoleptic indices, moisture weight fraction, changes in pH and water activity were analyzed before treatment and during storage. Sanitary microbiological analyses were carried out by the following indicators: quantity of mesophilic aerobic and facultative anaerobic microorganisms (QMAFAnM), the presence and quantity of coliforms, Salmonella, Escherichia coli, Listeria monocytogenes, Proteus. It was shown that meat cold treatment with argon plasma inhibited the development of mesophilic microorganisms. The colony forming units detected in the samples after ten days of storage were determined by the duration of exposure to plasma. It was proved that meat treatment for 15 and 30 min had the bactericidal effect and facilitated an improvement in meat color during storage. The organoleptic indices of the samples treated with plasma corresponded to the requirements of standards and approved consumer characteristics.
Quality properties and storage stability of beef burger as influenced by addition of orange peels (albedo)
Authors: Baioumy A. A. , Abedelmaksoud T. G. 
Published: April 2021

The objective of this study was to investigate the effect of using the orange albedo (OA) on the quality attributes of low fat beef burger. The analysis included: chemical composition, water-holding capacity, cooking loss% and pH values as well as sensory evaluation of low fat beef burger prepared with the introduction of the OA with a concentration of 5% as a partial fat replacer were also studied. Microbiological analysis of frozen minced meat semi-finished products (burger patties, at minus 18 °C) stored for 126 days was included. According to the obtained results, the OA contains 1.47% of lipids, 1.42% of protein and 24.61% of dietary fiber. The result also showed that the usage of OA has a positive impact on organoleptic indicators of beef burger. Nutritional value, waterholding capacity, cooking loss% and pH of treated burger patties were improved. In addition, the caloric value of treatment samples decreased because of replacing the animal fat with the OA in recipe. During the storage of semi-finished products, Thiobarbituric values (TBA) showed that treatment samples substantially developed using OA instead of animal fat had a lower level of lipid oxidation compared to control samples. The shelf life of treated burger patties was significantly increased compared to control sample by decreasing the microbial growth and rate of fatty acids oxidation. Finally, OA could be accepted as a functional component in meat products.
Biological-based control strategies for MBR membrane biofouling: a review
Authors: Cui Yin , Gao Huan , Yu Ran , Gao Lei , Zhan Manjun 
Published: April 2021

Abstract
Membrane bioreactor (MBR) technology has been paid extensive attention for wastewater treatment because of its advantages of high effluent quality and minimized occupation space and sludge production. However, the membrane fouling is always an inevitable problem, which causes high operation and maintenance costs and prevents the wide use of MBR technology. The membrane biofouling is the most complicated and has relatively slow progress among all types of fouling. In recent years, many membrane biofouling control methods have been developed. Different from the physical or chemical methods, the biological-based strategies are not only more effective for membrane biofouling control, but also milder and more environment-friendly and, therefore, have been increasingly employed. This paper mainly focuses on the mechanism, unique advantages and development of biological-based control strategies for MBR membrane biofouling such as quorum quenching, uncoupling, flocculants and so on. The paper summarizes the up-to-date development of membrane biofouling control strategies, emphasizes the advantages and promising potential of biological-based ones, and points out the direction for future studies.
A violência como regra
Authors: Souza Borzuk Cristiane , Carvalho Lombardi Tosta Tiago 
Published: April 2021

Resumo: O objetivo deste texto é analisar as determinações sociais e subjetivas que tornam as diferenças interpessoais ameaçadoras para os indivíduos, produzindo nestes o ódio e a intolerância. Investigou-se os entrelaçamentos entre indivíduo e sociedade que favorecem o surgimento de comportamentos intolerantes, tornando-os uma espécie de regra dentro da vida social regida pelo capitalismo administrado. Com a realização deste estudo foi possível verificar que há uma tendência inerente às sociedades administradas a promover o modo de funcionamento psíquico paranoico em seus indivíduos, resultando em uma lógica de violência e intolerância como um mecanismo para a manutenção da própria coerência interna. Ao tornar-se um “existencial social”, a paranoia deixa de ser uma categoria exclusivamente clínica para se tornar um modelo de administração da sociedade, produzindo e reproduzindo nas subjetividades as suas características fundamentais. O referencial teórico e metodológico é a Teoria Crítica da Sociedade.
Palavras-chave: Intolerância. Paranoia. Violência. Indivíduo. Sociedade.
Interfaces entre a Psicologia e o Direito Internacional
Authors: Andrade Vivenzio Rafaella , Borges Taveira Luís Felipe 
Published: April 2021

Atualmente, é possível encontrar um vasto e multidisciplinar acervo teórico sobre as populações infantis, que respaldam a elaboração das formas de cuidado que são voltadas para as crianças. Desde Convenções Internacionais até Decretos do Executivo, o Direito se preocupa com a proteção à criança. Simultaneamente, a Psicologia trata do assunto de maneira mais particularizada, levando em conta aspectos específicos necessários à sua eficaz proteção. O objetivo do trabalho é, então, traduzido na questão: as políticas públicas adotadas a fim de maximizar o bem estar infantil, admitidas no ordenamento jurídico, são encaradas a partir das especificidades subjetivas e particularizadas das crianças ou sob um prisma axiológico e principiológico geral? O objeto da pesquisa é o estudo da relação entre os instrumentos normativos nacionais e internacionais de proteção às crianças e as suas reais necessidades, levantadas pela Psicologia. A partir da pesquisa bibliográfica e utilizando um método qualitativo, alcançou-se o resultado de que os princípios e valores definidos na Convenção Sobre os Direitos da Criança harmonizam com a proteção conferida na Constituição Federal e no Estatuto da Criança e do Adolescente, todavia a sua execução exige uma vigilância às dimensões etária e regional aplicáveis. A conclusão, por sua vez, é de que os profissionais de outras áreas, como os psicólogos, são essenciais para que haja a efetiva proteção da criança, com a observância das particularidades regionais e necessidades especiais da idade.
Palavras-chave: Direito Internacional; Psicologia do Desenvolvimento; Infância; Estatuto da Criança e do Adolescente; Convenção Sobre os Direitos da Criança.