bluuebunny/crossref_metadata_2025_split huggingface dataset:
Linking sustainable business models and supply chains — Toward an integrated value creation framework
Authors: Norris Simon , Hagenbeck Julia , Schaltegger Stefan 
Published: June 2021

AbstractExtant literature on sustainable business models highlights that value creation stems from resources exchanged in relationships between a focal firm and its stakeholders. In this context, the literature has, so far, focused on direct relationships. However, despite the acknowledged relevance of sustainability issues in supply chains, this relational view of the focal company and its direct stakeholders has not been extended toward value creation for and with indirect stakeholders, such as stakeholders of suppliers. Addressing this gap, this conceptual article integrates a relational view of sustainable supply chain management into the management of sustainable business models. It extends the scope of sustainable business models from relationships between the focal firm and its direct stakeholders to indirect relationships with stakeholders of suppliers. A framework is developed that supports analysis and management of value‐creating relationships between the focal firm, suppliers, and stakeholders of suppliers. By extending the conceptualization of sustainable business models to consider relationship chains beyond direct relationships, this article proposes that a focal firm has to actively manage interactions both with suppliers and with suppliers' stakeholders.
Improvement of the efficiency and quality in developing a new CHO host cell line
Authors: Huhn Steven C. , Ou Yang , Tang Xiaoyan , Jiang Bo , Liu Ren , Lin Henry , Du Zhimei 
Published: June 2021

AbstractChinese hamster ovary (CHO) cells are a ubiquitous tool for industrial therapeutic recombinant protein production. However, consistently generating high‐producing clones remains a major challenge during the cell line development process. The glutamine synthetase (GS) and dihydrofolate reductase (DHFR) selection systems are commonly used CHO expression platforms based on controlling the balance of expression between the transgenic and endogenous GS or DHFR genes. Since the expression of the endogenous selection gene in CHO hosts can interfere with selection, generating a corresponding null CHO cell line is required to improve selection stringency, productivity, and stability. However, the efficiency of generating bi‐allelic genetic knockouts using conventional protocols is very low (<5%). This significantly affects clone screening efficiency and reduces the chance of identifying robust knockout host cell lines. In this study, we use the GS expression system as an example to improve the genome editing process with zinc finger nucleases (ZFNs), resulting in improved GS‐knockout efficiency of up to 46.8%. Furthermore, we demonstrate a process capable of enriching knockout CHO hosts with robust bioprocess traits. This integrated host development process yields a larger number of GS‐knockout hosts with desired growth and recombinant protein expression characteristics.
A New Apotirucallane from Walsura trichostemon Leaves and Its Antibacterial and α‐Glucosidase Inhibitory Activities
Authors: Suchaichit Nattawut , Pitchuanchom Siripit , Kanokmedhakul Kwanjai , Moosophon Panawan , Chompoosor Apiwat , Kanokmedhakul Somdej , Suchaichit Natcha P. 
Published: June 2021

AbstractPhytochemical investigation of Walsura trichostemon leaves led to the isolation of a new apotirucallane‐type triterpenoid, 11,25‐dideacetyl‐16‐hydroxytrichostemonate (1), along with two known apotirucallane‐type triterpenoids (2 and 3), two known tirucallane‐type triterpenes (4 and 5), and two known steroids (6 and 7). Their structures were identified by intensive analysis of 1D and 2D nuclear magnetic resonance, infrared, and mass spectrometry data, which were compared with data reported in the literature. Compounds 2, 3, and 5 exhibited moderate antibacterial activity against Pseudomonas aeruginosa (minimum inhibitory concentration (MIC) value: 64 μg/mL), and compound 4 showed weak antibacterial activity against P. aeruginosa (MIC: 128 μg/mL). Furthermore, compound 5 displayed activity against Bacillus cereus (MIC: 64 μg/mL). In addition, compound 4 showed stronger α‐glucosidase inhibitory activity than the control, acarbose. The active compound 4 was subjected to molecular docking experiments using AutoDock4 and revealed precise interactions with the active gorge of the enzyme through hydrogen bonding, supporting the in vitro results.
Phytochemical Profile and Antioxidant Activity of the Aerial Part Extracts from Matthiola incana subsp. rupestris and subsp. pulchella (Brassicaceae) Endemic to Sicily
Authors: Miceli Natalizia , Cavò Emilia , Spadaro Vivienne , Raimondo Francesco Maria , Ragusa Salvatore , Cacciola Francesco , El Majdoub Yassine Oulad , Arena Katia , Mondello Luigi , Condurso Concetta , Cincotta Fabrizio , Taviano Maria Fernanda 
Published: June 2021

AbstractAs part of a project aimed at investigating the Matthiola taxa endemic to Sicily (Italy), this study focused on Matthiola incana, an edible species used in the traditional medicine of various countries. Herein, the characterization of phenolic and volatile compounds, the antioxidant capacity in vitro (1,1‐diphenyl‐2‐picrylhydrazil (DPPH), reducing power and Fe2+ chelating activity assays) and the toxicity test (Artemia salina lethality bioassay) of the hydroalcoholic extracts from the aerial parts of M. incana subsp. rupestris from Mt. Pellegrino (Palermo) and Mt. Erice (Trapani), and of M. incana subsp. pulchella are reported. The results are compared with those previously shown for M. incana subsp. incana, to achieve a comprehensive overview of the three subspecies. The HPLC‐PDA/ESI‐MS and SPME‐GC/MS analyses led to the identification of 13 phenolics and 54 volatile compounds. Differences in the qualitative‐quantitative profile of these phytochemicals have been highlighted between the M. incana subspecies. The antioxidant tests showed different activity for the extracts, which were found to possess better chelating properties. At last, none of the tested extracts displayed toxicity against brine shrimp larvae. These findings enrich the knowledge on the Matthiola taxa growing wild in Sicily, both from the strictly systematic point of view and for the possible applications as sources bioactive compounds that can be used in the nutraceutical field.
Cloning and Functional Characterization of a Squalene Synthase from Paris polyphylla var. yunnanensis
Authors: Gao Jian‐Xiong , Chen Yue‐Gui , Li De‐Sen , Lin Liang , Liu Yan , Li Sheng‐Hong 
Published: June 2021

AbstractParis polyphylla Smith var. yunnanensis (Franch.) Hand. – Mazz. is a precious traditional Chinese medicine, and steroidal saponins are its major bioactive constituents possessing extensive biological activities. Squalene synthase (SQS) catalyzes the first dedicated step converting two molecular of farnesyl diphosphate (FDP) into squalene, a key intermediate in the biosynthetic pathway of steroidal saponins. In this study, a squalene synthase gene (PpSQS1) was cloned and functionally characterized from P. polyphylla var. yunnanensis, representing the first identified SQS from the genus Paris. The open reading frame of PpSQS1 is 1239 bp, which encodes a protein of 412 amino acids showing high similarity to those of other plant SQSs. Expression of PpSQS1 in Escherichia coli resulted in production of soluble recombinant proteins. Gas chromatography‐mass spectrometry analysis showed that the purified recombinant PpSQS1 protein could produce squalene using FDP as a substrate in the in vitro enzymatic assay. qRT‐PCR analysis indicated that PpSQS1 was highly expressed in rhizomes, consistent with the dominant accumulation of steroidal saponins there, suggesting that PpSQS1 is likely involved in the biosynthesis of steroidal saponins in the plant. The findings lay a foundation for further investigation on the biosynthesis and regulation of steroidal saponins, and also provide an alternative gene for manipulation of steroid production using synthetic biology.
COVID‐19 isolation drape for sialendoscopy‐assisted transfacial approach to parotid gland
Authors: Gaffuri Michele , Libonati Antonio , Ettori Jacopo , Torretta Sara , Pignataro Lorenzo , Capaccio Pasquale 
Published: June 2021

AbstractSurgical procedures requiring close contact with saliva, such as salivary gland surgery, may determine the risk of spreading the SARS‐CoV‐2 infection. The use of PPE and isolation settings are mandatory to protect health workers.
Infective endocarditis uncovered a very rare congenital heart disease
Authors: Jafari Fatemeh , Mirabdollahi Seyed‐Alireza , Jafari Shirin , Saberi Arezoo , Mohammadi Khadije 
Published: June 2021

AbstractIn this article, we introduced a case of rare congenital anomalies that was asymptomatic until adulthood and was complicated by infective endocarditis and dissection of aortic valve leaflet.
2q13 microdeletion syndrome: Report on a newborn with additional features expanding the phenotype
Authors: Piro Ettore , Serra Gregorio , Giuffrè Mario , Schierz Ingrid Anne Mandy , Corsello Giovanni 
Published: June 2021

AbstractWe describe an additional newborn with craniofacial dysmorphisms, congenital heart disease, hypotonia, and a 2q13 deletion of 1.7 Mb. The clinical and genomic findings observed are consistent with the diagnosis of 2q13 microdeletion syndrome.
Two novel biallelic variants in TECPR2 and FA2H genes causing complicated hereditary spastic paraplegia in Iranian families from Lur ethnicity: Case series
Authors: Edizadeh Masoud , Chegeninejad Negar , Akbari Soheila , Salehirad Maryam , Pakmanesh Rezvan , Ahmadipour Shokoufeh , Hayatigolkhatmi Kourosh , Khodadadi Hamidreza 
Published: June 2021

AbstractWe herein report first Iranian families with spastic paraplegia 35 and 49 and claim that TECPR2 gene causes complicated spastic paraplegia 49 with or without sensory autonomic neuropathy. In addition, we show how coexistence of SPG49 and griscelli syndrome can lead to misdiagnosis.
The CdIn2S4/WO3 Nanosheet Composite Has a Significantly Enhanced Photo‐electrochemical Cathodic Protection Performance and Excellent Electron Storage Capability
Authors: Yang Yuying , Chen Zhuoyuan , Feng Chang , Jing Jiangping 
Published: June 2021

AbstractPhoto‐electrochemical cathodic protection (CP) technology is considered to be a green metallic corrosion protection technology that uses solar energy to protect from corrosion and does not consume any anode materials. In this work, a CdIn2S4/WO3 nanocomposite photoelectrode was prepared, and its photo‐electrochemical CP performance and mechanism were studied and analyzed. WO3 has a well band matching with CdIn2S4, leading to a significantly enhanced photo‐electrochemical CP performance of the nanocomposite. Meanwhile, as confirmed in this work, the CdIn2S4/WO3 nanocomposite can store photoinduced electrons under light illumination through intercalation reactions and changing the valence state of tungsten. Moreover, it can discharge in the dark state to provide continuous CP for the coupled metals. This research will promote the practical application process of the photo‐electrochemical CP technology.
Nitric Oxide Photoreleasers with Fluorescent Reporting
Authors: Fraix Aurore , Parisi Cristina , Seggio Mimimorena , Sortino Salvatore 
Published: June 2021

AbstractNitric oxide (NO) plays a multifaceted role in human physiology and pathophysiology, and its controlled delivery has great prospects in therapeutic applications. The light‐activated uncaging of NO from NO caging compounds allows this free radical to be released with accurate control of site and dosage, which strictly determine its biological effects. Molecular constructs able to activate fluorescence concomitantly to NO release offer the important advantage of easy and real‐time tracking of the amount of NO uncaged in a non‐invasive fashion even in the cell environment. This contribution provides an overview of the advances in photoactivatable NO releasers bearing fluorescent reporting functionalities achieved in our and other laboratories, highlighting the rationale design and their potential therapeutic applications.
Various Molecular Arrangements Leading to Different Desolvation Rates: Research on the Stability of 2,7‐Dibromo‐9H‐carbazole Solvates
Authors: Guo Xuran , Chen Wei , Wei Rongli , Sui Jingchen , Huang Xin , Wang Na , Liu Songqiang , Wang Ting , Hao Hongxun 
Published: June 2021

AbstractThe stability of solvates is very important in the field of crystal engineering. Molecular interaction and packing mode are important factors affecting the stability of solvates. In this study, 2,7‐dibromo‐9H‐carbazole is selected as the model compound to investigate the influence of molecular stacking mode on the stability of solvates. An anhydrous form and five solvates of 2,7‐dibromo‐9H‐carbazole are obtained by recrystallization. The desolvation phenomena of the five solvates are studied by thermogravimetric analysis, hot stage microscope, and infrared spectroscopy and it is found that the stability of the solvates is N,N‐dimethylacetamide solvate > dimethyl sulfoxide solvate > N,N‐dimethylformamide solvate > dioxane solvate > acetonitrile solvate. Crystal structures are analyzed by single crystal X‐ray diffraction and Hirshfeld surface analysis is also applied to analyze the intermolecular interactions in the crystals. The results show that the stability of the five solvates is related to the packing modes of the molecules in the crystal. It is suggested that the solute molecules and solvent molecules in the unstable solvates are arranged in the interlayer mode, while they are arranged in a staggered mode in stable solvates.
Imine‐Induced Metal‐Organic and Covalent Organic Coexisting Framework with Superior Li‐Storage Properties and Activation Mechanism
Authors: Zhao Lu , Tang Xuxu , Lv Li‐Ping , Chen Shuangqiang , Sun Weiwei , Wang Yong 
Published: June 2021

AbstractDue to the adjustable structure and the broad application prospects in energy and other fields, the exploration of porous organic materials [metal‐organic polymers (MOPs), covalent organic frameworks (COFs), etc.] has attracted extensive attention. In this work, an imine‐induced metal‐organic and covalent organic coexisting framework (Co−MOP@COF) hybrid was designed based on the combination between the amino units from the organic ligands of Co−MOP and the aldehyde groups from COF. The obtained Co−MOP@COF hybrid with layer‐decorated microsphere morphology exhibited good electrochemical cycling performance (a large reversible capacity of 1020 mAh g−1 after 150 cycles at 100 mA g−1 and a reversible capacity of 396 mAh g−1 at 500 mA g−1) as the anode for Li‐ion batteries. The coexisting framework structure endowed the Co–MOP@COF hybrid with more surface area exposed in the exfoliated COF structure, which provided rapid Li‐ion diffusion, better electrolyte infiltration, and effective activation of functional groups. Therefore, the Co−MOP@COF hybrid material achieved an enhanced Li storage mechanism involving multi‐electron redox reactions, related to the CoII center and organic groups (C=C groups of benzene rings and C=N groups), and furthermore improved electrochemical performance.
Mapping supply of and demand for ecosystem services to assess environmental justice in New York City
Authors: Herreros‐Cantis Pablo , McPhearson Timon 
Published: June 2021

AbstractLivability, resilience, and justice in cities are challenged by climate change and the historical legacies that together create disproportionate impacts on human communities. Urban green infrastructure has emerged as an important tool for climate change adaptation and resilience given their capacity to provide ecosystem services such as local temperature regulation, stormwater mitigation, and air purification. However, realizing the benefits of ecosystem services for climate adaptation depend on where they are locally supplied. Few studies have examined the potential spatial mismatches in supply and demand of urban ecosystem services, and even fewer have examined supply–demand mismatches as a potential environmental justice issue, such as when supply–demand mismatches disproportionately overlap with certain socio‐demographic groups. We spatially analyzed demand for ecosystem services relevant for climate change adaptation and combined results with recent analysis of the supply of ecosystem services in New York City (NYC). By quantifying the relative mismatch between supply and demand of ecosystem services across the city we were able to identify spatial hot‐ and coldspots of supply–demand mismatch. Hotspots are spatial clusters of census blocks with a higher mismatch and coldspots are clusters with lower mismatch values than their surrounding blocks. The distribution of mismatch hot‐ and coldspots was then compared to the spatial distribution of socio‐demographic groups. Results reveal distributional environmental injustice of access to the climate‐regulating benefits of ecosystem services provided by urban green infrastructure in NYC. Analyses show that areas with lower supply–demand mismatch tend to be populated by a larger proportion of white residents with higher median incomes, and areas with high mismatch values have lower incomes and a higher proportion of people of color. We suggest that urban policy and planning should ensure that investments in “nature‐based” solutions such as through urban green infrastructure for climate change adaptation do not reinforce or exacerbate potentially existing environmental injustices.
Geography, Host Genetics, and Cross‐Domain Microbial Networks Structure the Skin Microbiota of Fragmented Brazilian Atlantic Forest Frog Populations
Authors: Belasen Anat M. , Riolo Maria A. , Bletz Molly C. , Lyra Mariana L. , Toledo L. Felipe , James Timothy Y. 
Published: June 2021

AbstractThe host‐associated microbiome plays a significant role in health. However, the roles of factors such as host genetics and microbial interactions in determining microbiome diversity remain unclear. We examined these factors using amplicon‐based sequencing of 175 Thoropa taophora frog skin swabs collected from a naturally fragmented landscape in southeastern Brazil. Specifically, we examined (1) the effects of geography and host genetics on microbiome diversity and structure; (2) the structure of microbial eukaryotic and bacterial co‐occurrence networks; and (3) co‐occurrence between microeukaryotes with bacterial OTUs known to affect growth of the fungal pathogen Batrachochytrium dendrobatidis (Bd). While bacterial alpha diversity varied by both site type and host MHC IIB genotype, microeukaryotic alpha diversity varied only by site type. However, bacteria and microeukaryote composition showed variation according to both site type and host MHC IIB genotype. Our network analysis showed the highest connectivity when both eukaryotes and bacteria were included, implying that ecological interactions may occur among domains. Lastly, anti‐Bd bacteria were not broadly negatively co‐associated with the fungal microbiome and were positively associated with potential amphibian parasites. Our findings emphasize the importance of considering both domains in microbiome research and suggest that for effective probiotic strategies for amphibian disease management, considering potential interactions among all members of the microbiome is crucial.
Consistent functional clusters explain the effects of biodiversity on ecosystem productivity in a long‐term experiment
Authors: Jaillard Benoît , Deleporte Philippe , Isbell Forest , Loreau Michel , Violle Cyrille 
Published: June 2021

AbstractBiomass production in ecosystems is a complex process regulated by several facets of biodiversity and species identity, but also species interactions such as competition or complementarity between species. For studying these different facets separately, ecosystem biomass is generally partitioned in two biodiversity effects. The composition effect is a simple, linear effect, and the interaction effect is a more subtle, nonlinear effect. Here we used a clustering approach (1) to separately and comprehensively capture all linear and nonlinear effects induced by both biodiversity effects on ecosystem functioning, and (2) to determine the functional composition at the origin of each biodiversity effect. We used data from the long‐term Cedar Creek BioDIV experiment carried out over 22 yr, and we partitioned multiplicatively the biomass in composition and interaction effects. Both biodiversity effects were weakly correlated. Our clustering approach accurately explains and predicts each diversity effect over time: each one is modeled by a different functional composition. Even if environmental conditions and the strength of interaction effect strongly varied over time, the functional clusters of species that govern the interaction effect do not change over the 22 yr of the experiment. The functional composition governing the interaction effect is therefore very robust. In contrast, the functional clusters of species that govern the composition effect are less robust and change with environmental conditions. Understanding ecosystem functioning therefore requires that ecological properties are first partitioned by type, then each type of property is analyzed and modeled separately. Approaches without a priori groupings of species, such as functional clustering, appear particularly efficient and robust to unravel the web of species interactions, and identify the role played by species on biodiversity effects.
Consequences of phenological shifts and a compressed breeding period in Magellanic penguins
Authors: Cappello Caroline D. , Boersma P. Dee 
Published: June 2021

AbstractPhenological shifts may ameliorate negative effects of climate change or create carryover effects and mismatches that decrease fitness. Identifying how phenological shifts affect performance is critical for understanding how individuals and populations will respond to climate change, but requires long‐term, longitudinal data. Using 34 yr of data from the Magellanic penguin (Spheniscus magellanicus) colony at Punta Tombo, Argentina, we examined the consequences of the delayed onset of breeding (i.e., arrival and egg‐laying dates) that has occurred at the colony since 1983. To understand how the delay propagates through the rest of the reproductive cycle, we identified phenological trends in hatch and fledge dates. Median hatch dates were 0.29 d later each year, amounting to a 10‐d shift over the course of the study. Median fledge dates did not shift over the 34‐yr period, however, thus shortening the median nestling period duration by 14%. We tested several predictions regarding performance outcomes of the compressed nestling period, finding that later‐hatched chicks fledged significantly younger than earlier‐hatched chicks, and that younger fledglings left the colony with smaller bills and with more chick down. Interestingly, although younger chicks fledged significantly lighter and in worse body condition than older fledglings early in the study, this trend reversed over time, with younger chicks actually fledging heavier and in better body condition in more recent years. Smaller and lighter fledglings were less likely to recruit to the colony as adults. We find that delayed breeding has significantly compressed nestling periods at Punta Tombo, influencing chick growth and fledgling condition. These findings highlight the importance of studying phenology across multiple life events to understand the consequences of phenological shifts for organismal fitness.