bluuebunny/crossref_metadata_2025_split huggingface dataset:
Oiling the gears of memory: quercetin exposure during memory formation, consolidation, and recall enhances memory in Lymnaea stagnalis
Authors: Rivi Veronica , Batabyal Anuradha , Benatti Cristina , Blom Johanna MC , Tascedda Fabio , Lukowiak Ken 
Published: June 2021

AbstractMemory formation (short-term, intermediate-term, and long-term) is an integral process of cognition which allows individuals to retain important information and is influenced by various intrinsic and extrinsic factors. A major extrinsic factor influencing cognition across taxa is diet, which may contain rich sources of molecular agents with antioxidant, anti-inflammatory, and memory enhancing properties that potentially enhance cognitive ability. A common and abundant flavonoid present in numerous food substances is quercetin (Q) which is also known to upregulate cyclic AMP response element binding protein (CREB) in several animals including our model system Lymnaea stagnalis. Since CREB is known to be involved in long term memory (LTM) formation, we investigated the role of Q-exposure on memory formation, consolidation, and recall during operant conditioning of aerial respiratory behaviour in Lymnaea. Snails were exposed to Q 3h before or after training to ascertain its effects on LTM. Additionally, we investigated the effect of the combined presentation of a single reinforcing stimulus (at 24h post-training or 24h before training) and Q-exposure on both LTM formation and reconsolidation. Our data indicate that Q-exposure acts on the different phases of memory formation, consolidation, and recall leading to enhanced LTM formation.Summary StatementQuercetin enhances long-term memory formation acting on the different phases of memory formation, consolidation, and recall.
Intramolecular carbon isotope signals reflect metabolite allocation in plants
Authors: Wieloch Thomas , Sharkey Thomas David , Werner Roland Anton , Schleucher Jürgen 
Published: June 2021

AbstractStable isotopes at natural abundance are key tools to study physiological processes occurring outside the temporal scope of manipulation and monitoring experiments. Whole-molecule carbon isotope ratios (13C/12C) enable assessments of plant carbon uptake yet conceal information about carbon allocation. Here, we identify an intramolecular 13C/12C signal at treering glucose C-5 and C-6 and develop experimentally testable theories on its origin. More specifically, we assess the potential of processes within C3 metabolism for signal introduction based (inter alia) on constraints on signal propagation posed by metabolic networks. We propose that the intramolecular signal reports carbon allocation into major metabolic pathways in actively photosynthesising leaf cells including the anaplerotic, shikimate, and non-mevalonate pathway. We support our theoretical framework by linking it to previously reported whole-molecule 13C/12C increases in cellulose of ozone-treated Betula pendula and a highly significant relationship between the intramolecular signal and tropospheric ozone concentration. Our theory postulates a pronounced preference of leaf-cytosolic triose-phosphate isomerase to catalyse the forward reaction in vivo (dihydroxyacetone phosphate to glyceraldehyde 3-phosphate). In conclusion, intramolecular 13C/12C analysis resolves information about carbon uptake and allocation enabling more comprehensive assessments of carbon metabolism than whole-molecule 13C/12C analysis.HighlightIntramolecular 13C/12C analysis resolves information about carbon uptake and allocation (and associated environmental controls) enabling more comprehensive assessments of carbon metabolism, plant-environment interactions, and environmental variability than whole-molecule 13C/12C analysis.
Nde1 is Required for Heterochromatin Compaction and Stability in Neocortical Neurons
Authors: Chomiak Alison A. , Lowe Clara C. , Guo Yan , McDaniel Dennis , Pan Hongna , Zhou Xiaoming , Zhou Qiong , Doughty Martin L. , Feng Yuanyi 
Published: June 2021

SUMMARYTheNDE1gene encodes a scaffold protein essential for brain development. While biallelicNDE1loss of function (LOF) causes microcephaly with profound mental retardation,NDE1missense mutations and copy number variations are associated with multiple neuropsychiatric disorders. However, the etiology of the diverse phenotypes resulting fromNDE1aberrations remains elusive. Here we show Nde1 controls neurogenesis through facilitating heterochromatin compaction via histone H4K20 trimethylation. This mechanism patterns diverse chromatin landscapes and stabilizes constitutive heterochromatin of neocortical neurons. We show NDE1 undergoes dynamic liquid-liquid phase separation, partitioning to the nucleus and interacting with pericentromeric and centromeric satellite repeats. Nde1 LOF results in nuclear architecture aberrations and DNA double strand breaks, as well as instability and derepression of pericentromeric satellite repeats in neocortical neurons. These findings uncover a pivotal role of NDE1/Nde1 in establishing and maintaining neuronal heterochromatin. They suggest that heterochromatin impairments underlie a wide range of brain dysfunction.
Clusters of neuronal Neurofascin prefigure node of Ranvier position along single axons
Authors: Vagionitis Stavros , Auer Franziska , Xiao Yan , Almeida Rafael G , Lyons David A , Czopka Tim 
Published: June 2021

SummaryThe spacing of nodes of Ranvier crucially affects conduction properties along myelinated axons. It has been assumed that node position is primarily driven by the growth of myelin sheaths. Here, we reveal an additional mechanism of node positioning that is driven by the axon. We show through longitudinal live imaging of node formation dynamics that stable clusters of the cell adhesion molecule Neurofascin A accumulate at specific sites along axons prior to myelination. While some of these clusters change position upon encounter with growing myelin sheaths, others restrict sheath extension and are therefore predictive of future node position. Animals that lack full-length Neurofascin A showed increased internodal distances and less regular spacing of nodes along single axons. Together, our data reveal the existence of an axonal mechanism to position its nodes of Ranvier that does not depend on regulation of myelin sheath length.
Characterisation of the transcriptional dynamics underpinning the function, fate, and migration of the mouse Anterior Visceral Endoderm
Authors: Thowfeequ Shifaan , Fiorentino Jonathan , Hu Di , Solovey Maria , Ruane Sharon , Whitehead Maria , Vanhaesebroeck Bart , Scialdone Antonio , Srinivas Shankar 
Published: June 2021

SUMMARYDuring early post-implantation development of the mouse embryo, the Anterior Visceral Endoderm (AVE) differs from surrounding visceral endoderm (VE) in its migratory behaviour and ability to restrict primitive streak formation to the opposite side of the egg cylinder. In order to characterise the molecular basis for the unique properties of the AVE, we combined single-cell RNA-sequencing of the VE prior to and during AVE migration, with high-resolution imaging, short-term lineage labelling, phosphoproteomics and pharmacological intervention. This revealed the transient nature of the AVE, the emergence of heterogeneities in AVE transcriptional states relative to position of cells, and its prominence in establishing gene expression asymmetries within the spatial constraints of the embryo. We identified a previously unknown requirement of Ephrin- and Semaphorin-signalling for AVE migration. These findings point to a tight coupling of transcriptional state and position in the AVE and reveal molecular heterogeneities underpinning its migratory behaviour and function.
Robotic industrial automation simulation-optimization for resolving conflict and deadlock
Authors: Fazlollahtabar Hamed , Saidi-Mehrabad Mohammad , Masehian Ellips 
Published: June 2021

Purpose
The purpose of this study is to investigate the benefits of the turning point layout; a simulation model being applicable for strategic level is designed that compares systems with and without turning points. Specifically, the avoidance of deadlocks and prevention of conflicts is substantial.

Design/methodology/approach
Optimization process for different layouts and configuration of autonomous guided vehicles (AGVs) are worked out using statistical methods for design parameters. Regression analysis is used to find effective design parameters and analysis of variance is applied for adjusting critical factors. Also, the optimal design is then implemented in a manufacturing system for an industrial automation and the results are reported.

Findings
The outputs imply the effectiveness of the proposed approach for real industrial cases. This research will combine both simulation-based method and optimization technique to improve the quality of solutions.

Originality/value
In AGV systems, the begin-end combinations are usually connected by using a fixed layout, which is not the optimal path. The capability of these configurations is limited and often the conflict of multiple AGVs and deadlock are inevitable. By appearing more flexible layouts and advanced technology, the positioning and dispatching of AGVs increased. A new concept would be to determine each path dynamically. This would use the free paths for AGVs leading to overcome the conflicts and deadlocks.
Three-dimensional tolerance analysis of discrete surface structures based on the torsor cluster model
Authors: Zhou Chuanyuan , Liu Zhenyu , Qiu Chan , Tan Jianrong 
Published: June 2021

Purpose
The purpose of this paper is to propose a novel mathematical model to present the three-dimensional tolerance of a discrete surface and to carry out an approach to analyze the tolerance of an assembly with a discrete surface structure. A discrete surface is a special structure of a large surface base with several discrete elements mounted on it, one, which is widely used in complex electromechanical products.

Design/methodology/approach
The geometric features of discrete surfaces are separated and characterized by small displacement torsors according to the spatial relationship of discrete elements. The torsor cluster model is established to characterize the integral feature variation of a discrete surface by integrating the torsor model. The influence and accumulation of the assembly tolerance of a discrete surface are determined by statistical tolerance analysis based on the unified Jacobian-Torsor method.

Findings
The effectiveness and superiority of the proposed model in comprehensive tolerance characterization of discrete surfaces are successfully demonstrated by a case study of a phased array antenna. The tolerance is evidently and intuitively computed and expressed based on the torsor cluster model.

Research limitations/implications
The tolerance analysis method proposed requires much time and high computing performance for the calculation of the statistical simulation.

Practical implications
The torsor cluster model achieves the three-dimensional tolerance representation of the discrete surface. The tolerance analysis method based on this model predicts the accumulation of the tolerance of components before their physical assembly.

Originality/value
This paper proposes the torsor cluster as a novel mathematical model to interpret the tolerance of a discrete surface.
Teaching a child with autism to request help only when needed
Authors: Chen Mo , Kreibich Shelley , Hyppa-Martin Jolene 
Published: June 2021

Purpose
Children with autism spectrum disorders (ASD) or other developmental disabilities are often reported to have challenges in well generalizing the newly learned communicative skills such as requesting help. Not requesting help when it is needed can hinder engagement and learning, whereas requesting help could also be socially inappropriate. This paper aims to offer a demonstration of applying general case instruction to teach a young child diagnosed with ASD to request help only when needed while concurrently increasing the child’s independence in task completion.

Design/methodology/approach
The demonstration adopted within-participant AB designs for one 5-year-old boy with ASD, with data collected across three tasks targeted for intervention and the other three tasks targeted for generalization probes throughout both the baseline and intervention phases. Dependent measures consisted of independent help request and independent task completion. Visual analysis was used to describe the results.

Findings
Results showed that the child with ASD learned to ask for help on difficult educational activities, while concurrently increasing his independence on these tasks; generalized the skill of requesting help by asking for help when he encountered other challenging novel tasks; and independently completed easy educational activities without requesting help.

Originality/value
The findings from this study may add to the limited literature that explored the generalization performance across tasks/activities in young learners with ASD, while demonstrating the feasibility of designing and applying general case instruction framework to enhance generalization performance for one individual learner.
The effects of mental budgeting on the intentions to switch to low-toxicity pesticides: evidence from vegetable farmers in Sichuan, China
Authors: Zeng Yangyi , Herzfeld Thomas 
Published: June 2021

PurposeMental budgeting, as a part of mental accounting theory, is expected to impact a household's budgetary management in terms of expenses. The purpose of this paper is to study whether and how mental budgeting can explain differences in farmers' reactions to different incentives of low-toxicity pesticide use.Design/methodology/approachBased on data from a survey of 393 vegetable farmers in the Sichuan Province, this analysis, using a Likert Scale approach, first explores whether farmers utilize mental budgeting. Secondly, using a Probit model, this paper analyzes how mental budgeting affects farmers' intentions to switch to low-toxicity pesticide use when faced with different incentives.FindingsThe results show that the majority of farmers categorize agricultural inputs into different groups and that 26.46% of the investigated farmers utilize mental budgeting for pest control practices. In addition, farmers who utilizing mental budgeting report a higher willingness to switch to low-toxicity pesticides when they're presented with a specific subsidy. Furthermore, if offered a price premium for quality, the willingness to switch to low-toxicity pesticides for farmers utilizing mentally budget is lower compared to other farmers.Originality/valueThis paper examines the existence of mental budgeting among farmers. It provides a better understanding of how farmers categorize agricultural inputs and their mental mechanisms with respect to agricultural expenses. Finally, this paper is the first to study the effects of mental budgeting on farmers' reactions to different incentives aimed at stimulating the adoption of low-toxicity pesticides.
Managing project scope creep in construction industry
Authors: Ajmal Mian M. , Khan Mehmood , Gunasekaran Angappa , Helo Petri T. 
Published: June 2021

PurposeProject scope creep is a nightmare and nearly intolerable task. Most project managers struggle to curtail the expanse and degree of scope creep. This study examines different likely project scope creep factors associated with the construction industry projects.Design/methodology/approachAfter many brainstorming sessions with construction stakeholders, several project scope creep factors were identified. Then, a detailed survey was executed in big construction projects of the United Arab Emirates (UAE). The data were analyzed using exploratory factor analysis (EFA) and confirmatory factor analysis (CFA).FindingsThe results derived and validated five conspicuous factors leading to project scope creep. Respectively, the highest and the lowest impact on project scope appears to be imposed by tasks/specifications and complexity/uncertainty.Practical implicationsIt offers crucial support to the project stakeholders in scrutinizing different factors that stand as hurdles to project success and allows them to seek remedies to resolve them.Originality/valueIt is among the first study in the region that identifies and validates the factors that hinder construction project success.
The changing nature of traditional work settings and the emerging virtual work environments in Africa: the experience of Nigerian women
Authors: Imhanrenialena Benedict Ogbemudia , Obi-anike Ozioma Happiness , Okafor Chikodili Nkiru , Ike Ruby Nneka 
Published: June 2021

PurposeThis paper aims to investigate work–life balance and job satisfaction in the emerging virtual work environments among women in patriarchal Nigerian society.Design/methodology/approachData were collected with structured and semi-structured questionnaire from 316 participants who signed up for online affiliate marketing programs in Nigeria. The data were analysed with descriptive statistics, while the hypotheses were tested with partial least squares structural equation modelling.FindingsThe outcomes indicate insignificant conflict in the interface between remotely working from home and the discharge of family care responsibilities among married Nigerian women. Also, the women derive significant job satisfaction from virtual work settings. Outcomes from the semi-structured interviews indicate that Nigerian women receive more support in indoor household chores than outdoor household chores while performing virtual work duties from home locations with housemaids being the highest source of such support.Originality/valueThis study extends work–life balance literature from the traditional work environments to the emerging virtual work settings in Africa by providing empirical evidence that the emerging virtual work settings do not result in work–family conflict but rather yield significant job satisfaction among Nigerian women.
Sport rebranding: the effect of different degrees of sport logo redesign on brand attitude and purchase intention
Authors: Williams Antonio S. , Son Sungwook 
Published: June 2021

PurposeDespite its growth, there has been a lack of investigation on how sport rebranding influences fans and their behaviors. This study sought to examine how a critical aspect of rebranding – logo redesign – influences fans' attitudes toward a sport brand and intent to purchase merchandise bearing the rebranded logo. More specifically, we aim to investigate the influence of varying degrees of logo change and fan identification in relation to brand attitude and purchase intention.Design/methodology/approachAn experimental design, 4 (degrees of logo change) × 2 (fan identification: low, high), was utilized with the sample of 277 participants. The current logo of the Ultimate Fighting Championship was redesigned into three fictitious rebranded logos as stimuli based on different rebranding types.FindingsThe results suggest that color change and revolutionary change generate the most negative attitudes from both high- and low-identified fans, and fan identification is not a significant factor influencing fan attitudes. Regarding purchase intention, low-identified fans are not significantly influenced by logo changes whereas highly identified fans showed similar response to their attitudes.Originality/valueThe findings advance the current body of knowledge on sport rebranding and fan behavior by demonstrating the effects of varying degrees of logo redesign and fan identification levels on brand attitude and purchase intention. Moreover, the current research has important implications for sport marketers on developing effective rebranding and logo redesign strategies.
Value trace problems with assisting references for Python programming self-study
Authors: Hay Mar Shwe San Hay Mar , Funabiki Nobuo , Syaifudin Yan Watequlis , Tar Phyu Phyu , Kyaw Htoo Htoo Sandi , Thant Hnin Aye , Kao Wen-Chung , Min Nandar Win , Myint Thandar , Htet Ei Ei 
Published: June 2021

Purpose
This study aims to present the value trace problem (VTP) for Python programming self-study, by extending the works for Java programming learning assistant system. In total, 130 VTP instances are generated using Python codes in textbooks and websites that cover basic/advanced grammar topics, fundamental data structures and algorithms and two common library usages. Besides, assisting references on Python programming topics related to the VTP instances are introduced to assist novice learners in solving them efficiently.

Design/methodology/approach
PyPLAS offers the VTP to study grammar topics and library usage through code reading. A VTP instance asks a learner to trace the actual values of important variables or output messages in the given source code. The correctness of any answer is checked through string matching.

Findings
The applications to 48 undergraduate students in Myanmar and Indonesia confirm the validity of the proposal in Python programming self-studies by novice learners.

Originality/value
The applications to 48 undergraduate students in Myanmar and Indonesia confirm the validity of the proposal in Python programming self-studies by novice learners.
Spirituality and entrepreneurship: integration of spiritual beliefs in an entrepreneurial journey
Authors: Rashid Sumayya , Ratten Vanessa 
Published: June 2021

Purpose
The role of spirituality in entrepreneurship is a new area of inquiry that has emerged from different scholarly fields. There is no specific way to study spirituality as it rather depends on the context and influence of religious beliefs. This paper aims to study the integration of spirituality in the entrepreneurial journeys of individual entrepreneurs in Pakistan. Thereby focusing on the factors that set reassurance for entrepreneurs to practice their spiritual beliefs in an emerging market context.

Design/methodology/approach
Using a qualitative research approach and data from entrepreneurs in different businesses, this study examines how entrepreneurs reach the point where they are willing to run their businesses based on their spiritual beliefs. The data analysis technique used to study the entrepreneurs is the Gioia method of analysis, which enables key themes to emerge.

Findings
This study finds that the process through which Pakistani entrepreneurs run their businesses based on spiritual beliefs within their mission and goals can be considered as discerning spiritual beliefs, affirmation of spiritual beliefs and materializing beliefs into action. Each of these spiritual beliefs has been accumulated through lifelong learning and experience in which trust in business relationships is highlighted.

Social implications
The insights of this study can be applied to different entrepreneurial ventures to create a framework of entrepreneurial businesses that incorporate spirituality within their mission and vision statements. It can then be used to design education and training activities for other spiritual entrepreneurs to enable further entrepreneurial development in different communities.

Originality/value
Spirituality can alter the entrepreneurial nature of a business. This means that entrepreneurs who possess high spiritual beliefs can incorporate their spirituality within specific business functions to enable a more positive outcome. This study stands as the first in Pakistan to consider the ways entrepreneurs incorporate their spiritual beliefs in their entrepreneurial journey.