bluuebunny/crossref_metadata_2025_split huggingface dataset:
A cultural experience to support mental health in people aged 16–24 during the COVID-19 pandemic compared to a typical museum website: study protocol of an online randomised controlled trial
Authors: Syed Sheriff Rebecca J. , Vuorre Matti , Riga Evgenia , Przybylski Andrew K. , Adams Helen , Harmer Catherine J. , Geddes John R. 
Published: July 2021

Abstract
Background
Despite the high prevalence of common mental disorders in adolescents and young adults, and their association with poor health and socio-economic outcomes throughout the lifespan, many young people do not seek or receive help for such disorders. There is growing interest in the community sector in supporting mental health in young people; however, there is little by way of experimental research in this area. During the COVID-19 pandemic and lockdown, we designed an online cultural experience to reduce anxiety and depression and support mental health in people aged 16–24.

Methods/design
The O-ACE POP (Online Active Community Engagement Proof of Principle) study is a UK-based online randomised controlled trial of an online cultural experience named Ways of Being, involving human centred narratives and viewpoints, compared with a typical museum website (the Ashmolean Museum). We aim to compare efficacy on affect, symptoms of epression and anxiety, flourishing and loneliness as well as investigating potential mechanisms of action.

Discussion
The COVID-19 pandemic has provided a unique opportunity to design an innovative approach to supporting mental health in young adults. Findings derived from this study will allow us to evaluate the efficacy of this intervention and will inform the design of studies to further refine the resource and test it further.

Trial registration
ClinicalTrials.gov NCT04663594. Registered on 11 December 2020 (submitted in same form 27 November 2020). Protocol v1.0: 27 November 2020. Date recruitment began: 4 December 2020. Recruitment complete (estimate): February 2021
Effect of opposing needling on motor cortex excitability in healthy participants and in patients with post-stroke hemiplegia: study protocol for a single-blind, randomised controlled trial
Authors: Xu Mindong , Zi Yinyu , Wu Jianlu , Xu Nenggui , Lu Liming , Liu Jiahui , Yu Yanling , Mo Haofeng , Wen Weifeng , Tang Xiaorong , Fan Wenjuan , Zhang Yu , Liu Churong , Yi Wei , Wang Lin 
Published: July 2021

Abstract
Background
Opposing needling has an obvious curative effect in the treatment of post-stroke hemiplegia; however, the mechanism of the opposing needling in the treatment of post-stroke hemiplegia is still not clear. The purpose of this study is to investigate the effect of opposing needling on the excitability of primary motor cortex (M1) of healthy participants and patients with post-stroke hemiplegia, which may provide insight into the mechanisms of opposing needling in treating post-stroke hemiplegia.

Methods
This will be a single-blind, randomised, sham-controlled trial in which 80 healthy participants and 40 patients with post-stroke hemiplegia will be recruited. Healthy participants will be randomised 1:1:1:1 to the 2-Hz, 50-Hz, 100-Hz, and sham electroacupuncture groups. Patients with post-stroke hemiplegia will be randomised 1:1 to the opposing needling or conventional treatment groups. The M1 will be located in all groups by using neuroimaging-based navigation. The stimulator coil of transcranial magnetic stimulation (TMS) will be moved over the left and right M1 in order to identify the TMS hotspot, followed by a recording of resting motor thresholds (RMTs) and motor-evoked potentials (MEPs) of the thenar muscles induced by TMS before and after the intervention. The primary outcome measure will be the percent change in the RMTs of the thenar muscles at baseline and after the intervention. The secondary outcome measures will be the amplitude (μV) and latency (ms) of the MEPs of the thenar muscles at baseline and after the intervention.

Discussion
The aim of this trial is to explore the effect of opposing needling on the excitability of M1 of healthy participants and patients with post-stroke hemiplegia.

Trial registration
Chinese Clinical Trial Registry ChiCTR1900028138. Registered on 13 December 2019.
Abdominal angiostrongyliasis in the Americas: fifty years since the discovery of a new metastrongylid species, Angiostrongylus costaricensis
Authors: Rojas Alicia , Maldonado-Junior Arnaldo , Mora Javier , Morassutti Alessandra , Rodriguez Rubens , Solano-Barquero Alberto , Tijerino Anamariela , Vargas Marianela , Graeff-Teixeira Carlos 
Published: July 2021

AbstractAngiostrongylus costaricensis is a zoonotic parasitic nematode described for the first time in 1971 by Pedro Morera and Rodolfo Céspedes in Costa Rica. This parasite causes an infection known as abdominal angiostrongyliasis, affecting mainly school-aged children and young adults. Infection with A. costaricensis has been associated with a myriad of rodent and mollusk species in the Americas and the Caribbean, as its natural hosts and reservoirs. In this commemorative review, we highlight the extensive research collected through a 50-year journey, which includes ecological, pathological, and molecular studies on A. costaricensis and its implicated disease. We also identify major knowledge gaps in its evolutionary history, the ecological role of imported and invasive mollusk species, and immune response. We propose that the advent of -omics analyses will allow us to gather novel information regarding A. costaricensis biology and infection dynamics, as well as to promote the design of much-needed sensitive and specific diagnostic tools.
Methylation status of nc886 epiallele reflects periconceptional conditions and is associated with glucose metabolism through nc886 RNAs
Authors: Marttila Saara , Viiri Leena E. , Mishra Pashupati P. , Kühnel Brigitte , Matias-Garcia Pamela R. , Lyytikäinen Leo-Pekka , Ceder Tiina , Mononen Nina , Rathmann Wolfgang , Winkelmann Juliane , Peters Annette , Kähönen Mika , Hutri-Kähönen Nina , Juonala Markus , Aalto-Setälä Katriina , Raitakari Olli , Lehtimäki Terho , Waldenberger Melanie , Raitoharju Emma 
Published: July 2021

Abstract
Background
Non-coding RNA 886 (nc886) is coded from a maternally inherited metastable epiallele. We set out to investigate the determinants and dynamics of the methylation pattern at the nc886 epiallele and how this methylation status associates with nc886 RNA expression. Furthermore, we investigated the associations between the nc886 methylation status or the levels of nc886 RNAs and metabolic traits in the YFS and KORA cohorts. The association between nc886 epiallele methylation and RNA expression was also validated in induced pluripotent stem cell (iPSC) lines.

Results
We confirm that the methylation status of the nc886 epiallele is mostly binomial, with individuals displaying either a non- or hemi-methylated status, but we also describe intermediately and close to fully methylated individuals. We show that an individual’s methylation status is associated with the mother’s age and socioeconomic status, but not with the individual’s own genetics. Once established, the methylation status of the nc886 epiallele remains stable for at least 25 years. This methylation status is strongly associated with the levels of nc886 non-coding RNAs in serum, blood, and iPSC lines. In addition, nc886 methylation status associates with glucose and insulin levels during adolescence but not with the indicators of glucose metabolism or the incidence of type 2 diabetes in adulthood. However, the nc886-3p RNA levels also associate with glucose metabolism in adulthood.

Conclusions
These results indicate that nc886 metastable epiallele methylation is tuned by the periconceptional conditions and it associates with glucose metabolism through the expression of the ncRNAs coded in the epiallele region.
Hypoxic preconditioning rejuvenates mesenchymal stem cells and enhances neuroprotection following intracerebral hemorrhage via the miR-326-mediated autophagy
Authors: Liu Jianyang , He Jialin , Ge Lite , Xiao Han , Huang Yan , Zeng Liuwang , Jiang Zheng , Lu Ming , Hu Zhiping 
Published: July 2021

Abstract
Background
Intracerebral hemorrhage (ICH) is a major public health concern, and mesenchymal stem cells (MSCs) hold great potential for treating ICH. However, the quantity and quality of MSCs decline in the cerebral niche, limiting the potential efficacy of MSCs. Hypoxic preconditioning is suggested to enhance the survival of MSCs and augment the therapeutic efficacy of MSCs in ICH. MicroRNAs (miRNAs) are known to mediate cellular senescence. However, the precise mechanism by which miRNAs regulate the senescence of hypoxic MSCs remains to be further studied. In the present study, we evaluated whether hypoxic preconditioning enhances the survival and therapeutic effects of olfactory mucosa MSC (OM-MSC) survival and therapeutic effects in ICH and investigated the mechanisms by which miRNA ameliorates hypoxic OM-MSC senescence.

Methods
In the in vivo model, ICH was induced in mice by administration of collagenase IV. At 24 h post-ICH, 5 × 105 normoxia or hypoxia OM-MSCs or saline was administered intracerebrally. The behavioral outcome, neuronal apoptosis, and OM-MSC survival were evaluated. In the in vitro model, OM-MSCs were exposed to hemin. Cellular senescence was examined by evaluating the expressions of P16INK4A, P21, P53, and by β-galactosidase staining. Microarray and bioinformatic analyses were performed to investigate the differences in the miRNA expression profiles between the normoxia and hypoxia OM-MSCs. Autophagy was confirmed using the protein expression levels of LC3, P62, and Beclin-1.

Results
In the in vivo model, transplanted OM-MSCs with hypoxic preconditioning exhibited increased survival and tissue-protective capability. In the in vitro model, hypoxia preconditioning decreased the senescence of OM-MSCs exposed to hemin. Bioinformatic analysis identified that microRNA-326 (miR-326) expression was significantly increased in the hypoxia OM-MSCs compared with that of normoxia OM-MSCs. Upregulation of miR-326 alleviated normoxia OM-MSC senescence, whereas miR-326 downregulation increased hypoxia OM-MSC senescence. Furthermore, we showed that miR-326 alleviated cellular senescence by upregulating autophagy. Mechanistically, miR-326 promoted the autophagy of OM-MSCs via the PI3K signaling pathway by targeting polypyrimidine tract-binding protein 1 (PTBP1).

Conclusions
Our study shows that hypoxic preconditioning delays OM-MSC senescence and augments the therapeutic efficacy of OM-MSCs in ICH by upregulating the miR-326/PTBP1/PI3K-mediated autophagy.
hucMSC-derived exosomes attenuate colitis by regulating macrophage pyroptosis via the miR-378a-5p/NLRP3 axis
Authors: Cai Xiu , Zhang Zhi-yu , Yuan Jin-tao , Ocansey Dickson Kofi Wiredu , Tu Qiang , Zhang Xu , Qian Hui , Xu Wen-rong , Qiu Wei , Mao Fei 
Published: July 2021

Abstract
Background
Human umbilical cord mesenchymal stem cell (hucMSC)-derived exosomes are recognized as novel cell-free therapeutic agents for inflammatory bowel disease (IBD), a condition caused by dysregulated intestinal mucosal immunity. In this event, macrophage pyroptosis, a process of cell death following the activation of NLRP3 (NOD-like receptor family, pyrin domain-containing 3) inflammasomes, is believed to partially account for inflammatory reactions. However, the role of macrophage pyroptosis in the process of hucMSC-derived exosomes alleviating colitis remains unknown. This study aimed at exploring the therapeutic effect and mechanism of hucMSC-derived exosomes on colitis repair.

Methods
In vivo, we used BALB/c mice to establish a dextran sulfate sodium (DSS)-induced colitis model and administrated hucMSC-derived exosomes intravenously to estimate its curative effect. Human myeloid leukemia mononuclear (THP-1) cells and mouse peritoneal macrophages (MPMs) were stimulated with lipopolysaccharides (LPS) and Nigericin to activate NLRP3 inflammasomes, which simulated an inflammation environment in vitro. A microRNA mimic was used to verify the role of miR-378a-5p/NLRP3 axis in the colitis repair.

Results
hucMSC-derived exosomes inhibited the activation of NLRP3 inflammasomes in the mouse colon. The secretion of interleukin (IL)-18, IL-1β, and Caspase-1 cleavage was suppressed, resulting in reduced cell pyroptosis. The same outcome was observed in the in vitro cell experiments, where the co-culture of THP-1 cells and MPMs with hucMSC-derived exosomes caused decreased expression of NLRP3 inflammasomes and increased cell survival. Furthermore, miR-378a-5p was highly expressed in hucMSC-derived exosomes and played a vital function in colitis repair.

Conclusion
hucMSC-derived exosomes carrying miR-378a-5p inhibited NLRP3 inflammasomes and abrogated cell pyroptosis to protect against DSS-induced colitis.
Dental health of preschool children after two-years of a supervised tooth brushing program in Southern Israel
Authors: Natapov Lena , Dekel Dan , Pikovsky Vadim , Zusman Shlomo Paul 
Published: July 2021

Abstract
Background
Supervised tooth brushing is an important part of leading national oral health improvement programs in different countries.
With the cessation of water fluoridation in 2014, a new program was immediately required to provide community-based caries prevention, especially amongst young children.
The aim of this study was to determine whether a supervised tooth brushing program (STBP) in kindergartens could reduce dental caries amongst preschool children, when compared with children from the same community who did not participate in the program. The study was performed 2 years after the start of the program.

Methods
Two Jewish and two Arab local authorities (one participating and one control) were randomly chosen. In each local authority, 4 kindergartens (children aged 5) were randomly chosen, giving a total of 16 kindergartens. Children in the intervention group brushed once daily at kindergartens, with fluoridated toothpaste, for two school- years. All the children were examined using the WHO Oral Health Survey Methods Ed.4.

Results
Two hundred eighty-three five-year-old children were examined, 157 of them Jewish (86 participants in STBP, 71 non-participants) and 126 Arab (59 vs 67 respectively). Among Jewish children, the fraction of untreated decayed teeth was 61% in the participant group and 65% for non-participants, and amongst the Arab children 69% vs. 90% respectively. The fraction of treated decayed teeth for the participant group was 37% compared to 29% for the non-participants among Jewish children, whilst for the Bedouin group it was 23% vs. 8% respectively.

Conclusions
After 2 years, supervised tooth brushing with fluoride toothpaste shows a favorable effect. This study suggests that dental health of children participating in STBP was better than the control group. Fewer carious teeth and more treated carious lesions were recorded in this group. This program can be applied to low SES communities nationwide.
Guidelines for fluoride concentration in toothpaste for children should be re-considered based on high caries levels.
Dynamical analysis of a stochastic three-species predator–prey system with distributed delays
Authors: Zhao Jinxing , Shao Yuanfu 
Published: July 2021

AbstractA stochastic two-prey one-predator system with distributed delays is proposed in this paper. Firstly, applying the linear chain technique, we transform the predator–prey system with distributed delays to an equivalent system with no delays. Then, by use of the comparison method and the inequality technique, we investigate the stability in mean and extinction of species. Further, by constructing some suitable functionals, using M-matrix theory and three important lemmas, we establish sufficient conditions assuring the existence of distribution and the attractivity of solutions. Finally, some numerical simulations are given to illustrate the main results.
Implications of food-for-work programs for consumption and production diversity: Evidence from the Tigray Region of Ethiopia
Authors: Debela Bethelhem Legesse , Shively Gerald E. , Holden Stein T. 
Published: July 2021

AbstractFood-based transfer programs have the potential to change diets or alter basic crop mixes. This study empirically investigates the associations between participating in food-for-work (FFW) programs and the diversity of food consumption and production. Four waves of panel data from the Tigray Region of Northern Ethiopia, covering the period 2001–2010, are used to estimate a series of panel data regressions. A dose-response model is used to measure how the intensity of FFW participation aligns with dietary outcomes. Results show that FFW participants had greater household dietary diversity compared with non-participants, with an average magnitude equivalent to one-fifth of a standard deviation in the food variety score. When items directly provided by the FFW program are excluded from the variety score, the overall effect is statistically weaker, but similar in sign and magnitude, suggesting modest “crowding in” of dietary diversity from FFW participation. FFW participation was not correlated with changes in production diversity, suggesting that the labor demands of the program did not alter crop choice. Findings have relevance for interventions that aim to improve food security and promote dietary quality in low-income populations.
A systematic review of enteral feeding by nasogastric tube in young people with eating disorders
Authors: Hindley Kristen , Fenton Clare , McIntosh Jennifer 
Published: July 2021

Abstract
Background
Adolescents with severe restrictive eating disorders often require enteral feeding to provide lifesaving treatment.
Nasogastric feeding (NG) is a method of enteral nutrition often used in inpatient settings to treat medical instability, to supplement poor oral intake or to increase nutritional intake. This systematic review sets out to describe current practice of NG in young people with eating disorders.

Methods
A systematic review following PRISMA guidelines was conducted by searching AMED, EMBASE and MEDLINE databases from 2000 to 2020. Inclusion terms were: enteral feeding by nasogastric tube, under 18 years, eating disorders, and primary research. Exclusion terms: psychiatric disorders other than eating disorders; non-primary research; no outcomes specific to NG feeding and participants over 18 years. Titles and abstracts were screened by all authors before reviewing full length articles. Quality assessment, including risk of bias, was conducted by all authors.

Results
Twenty-nine studies met the full criteria. 86% of studies were deemed high or medium risk of bias due to the type of study: 34.4% retrospective cohort and 10.3% RCT; 17.2% were qualitative. Studies identified 1) a wide range of refeeding regimes depending on country, settings, and the reason for initiation; 2) standard practice is to introduce Nasogastric feeds (NG) if medically unstable or oral intake alone is inadequate; 3) NG may enable greater initial weight gain due to increased caloric intake; 4) there are 3 main types of feeding regime: continuous, nocturnal and bolus; 5) complications included nasal irritation, epistaxis, electrolyte disturbance, distress and tube removal; 6) where NG is routinely implemented to increase total calorie intake, length of stay in hospital may be reduced; however where NG is implemented in correlation to severity of symptoms, it may be increased; 7) both medical and psychiatric wards most commonly report using NG in addition to oral intake.

Conclusions
NG feeding is a safe and efficacious method of increasing total calorie intake by either supplementing oral intake or continuously. There are currently no direct comparisons between continuous, nocturnal or bolus regimes, which may be used to direct future treatment for YP with ED.
Hedychium gardnerianum Sheph. ex Ker Gawl. from its discovery to its invasive status: a review
Authors: Pereira Maria João , Eleutério Telmo , Meirelles Maria Gabriela , Vasconcelos Helena Cristina 
Published: July 2021

AbstractHedychium gardnerianumSheph. ex Ker Gawl. is one of the 100 world's worst invasive alien species and the research target in areas as diverse as biological control, natural fibres uses, taxonomy or the biological activity of its compounds. This review aimed to clarify the taxonomic status and the native range ofH. gardnerianumand bring accuracy to the history of its introduction and escape from cultivation through the analysis of the increasing number of accessible digitalized dry specimens and grey literature. The analysis of the available information allowed to conclude that: (a)Hedychium gardnerianumis a validly published name, the authority of the name is Sheph. ex Ker Gawl., the species holotype is the illustration published along with the species name, and the Natural History Museum BM000574691 specimen collected in 1815 is the first dried specimen ofH. gardnerianum; (b) This species is native to the Central and Eastern Nepal, Bhutan, Northeast India and North Myanmar; (c) The species was cultivated at Cambridge Botanical Garden since 1818 and the first known herbarium specimen collected in Europe dates back to 1821; (d) Kathmandu (Nepal) and Khasi Hills (India) specimens are considered two varieties of the same species and the BM000574691 specimen is the lectotype ofH. gardnerianumvar.speciosum; (e) Specimens, references, and/or pictures support thatH. gardnerianumescaped from cultivation at Galicia (Spain), Azores archipelago, Madeira, Tenerife, Cuba, Jamaica, Martinique, Trinidad, Ascension, Mexico, Honduras, Brazil, South Africa, Swaziland, Zimbabwe, Réunion, Mauritius, Australia, New Zealand, Fiji, Hawaii, and Vietnam; and (f)H. gardnerianumis a serious pest in Azores, Madeira, Jamaica, Réunion, New Zealand and Hawaii and continues to expand its distribution area in South and Central America, Australia and Southern Africa. This review presents linear raw information compiled with precision, allowing the world databases updating their data but also gives the most detailed information possible to each country/region identifying new regions of concern and updating the invasiveness status in each region.
Enhancing argumentation component classification using contextual language model
Authors: Hidayaturrahman  , Dave Emmanuel , Suhartono Derwin , Arymurthy Aniati Murni 
Published: July 2021

AbstractArguments facilitate humans to deliver their ideas. The outcome of the discussion heavily relies on the validity of the argument. If an argument is well-composed, it is more effective to grasp the core idea behind the argument. To grade the argument, machines can be utilized by decomposing into semantic label components. In natural language processing, multiple language models are available to perform this task. It is divided into context-free and contextual models. The majority of previous studies used hand-crafted features to perform argument component classification, while state of the art language models utilize machine learning. The majority of these language models ignore the context in an argument. This research paper aims to analyze whether by including the context in the classification process may improve the accuracy of the language model which will enhance the argumentation mining process as well. The same document corpus is fed into several language models. Word2Vec and GLoVe represent the context free models, while BERT and ELMo as context sensitive language models. Accuracy and time from each model are then compared to determine the importance of context. The result shows that contextual language models are proven to be able to boost classification accuracy by approximately 20%. However, time comes as a cost where contextual models require longer training and prediction time. The benefit from the increase in accuracy outweighs the burden of time. Thus, as a contextual task, argumentation mining is suggested to use contextual model where context must be included to achieve promising results.