bluuebunny/crossref_metadata_2025_split huggingface dataset:
Surgical Compliance and Survival Outcomes For Patients With Osteosarcoma: A Population-Based Database Study
Authors: Tang Chao , Wang Dongdong , Zhang Hailong 
Published: August 2021

Abstract
PurposeWe aimed to determine the effect of surgical compliance on prognosis in patients with osteosarcoma and the risk factors leading to surgical noncompliance.MethodsWe analyzed the date collected 3412 osteosarcoma patients from the Surveillance Epidemiology and End Results (SEER) databases between 1973 and 2015. Cox analyses were used to identify the independent prognostic factors. Logistic regression model was conducted to clear the factors associated to surgical compliance; Kaplan-Meier estimator method was adopted to analyze the Overall survival (OS) and Cancer-specific survival (CSS). ResultsAmong 3412 eligible osteosarcoma patients, the poor surgical compliance of patients with osteosarcoma is associated with the earlier time of diagnosis, advanced age, lower economic income, poor grade, distant stage, accepting radiotherapy and refusing chemotherapy. There were significant differences in the effects of diagnostic time, age, grade, radiotherapy, chemotherapy, tumor stage and economic income on surgical compliance (All P < 0.05). Patients’ compliance was an independent prognostic factor for OS and CSS of osteosarcoma patients. ConclusionsOsteosarcoma patients with good surgical compliance have favorable survival. This can help clinicians effectively realize patients' views on surgery and guide patients to learn the signification of surgery.
Contact Model And Simulation Analysis of Rail Grinding With Open-Structured Abrasive Belt Based On Pressing Plate
Authors: Qian Chang , Wu Zhiwei , Hou Guangyou , Fan Wengang 
Published: August 2021

Abstract
Recently, the emerging rail grinding method with open-structured abrasive belt based on pressing plate which adopts full contour copying grinding method to extend the working length of abrasive belt and is more suitable for preventative grinding and maintenance of rail corrugation in high-speed railway has been proposed. The essence of its grinding process is the complex nonlinear interaction among the pressing plate, abrasive belt and rail. The research on the contact mechanism of the rail grinding method with open-structured abrasive belt based on pressing plate is still very lacking, which limits the practical application of the emering rail grinding method to a certain extent. In this paper, a theoretical model of the contact between pressing plate, abrasive belt and rail is established. The contact area morphology and contact stress distribution were obtained, and validity of the theoretical model was verified by finite element simulation. At the same time, the distribution characteristics of contact stress under single concentrated force, uniform force and multiple concentrated force were studied based on the contact model. It is concluded that the multiple concentrated force is the optimal loading method, and the contact stress is more uniform, which is more conducive to repair the standard contour of rail.
Daily Tackling Heath Inequalities: French Child Individual-Level Deprivation Index Development and Validation
Authors: Laporte Remi , Babe Philippe , Jouve Elisabeth , Daguzan Alexandre , Mazoue Franck , Minodier Philippe , Noel Guilhem , Urbina Diego , Gentile Stephanie 
Published: August 2021

Abstract
The purpose of this study was to develop and validate a pediatric individual-level index for deprivation, usable in clinical practice and in public health. The index had a 4 phases development: items generation with literature review and experts interviews, items reduction with steering committee consensus, index derivation with multivariate analysis, and index validation with psychometric and Pearson analysis. French Child Individual-Level Deprivation Index (FrenChILD-Index) was addressed by untrained healthcare professionals in a cross-sectional multicentric study. The deprivation burden was blindly evaluated in every domain of lifestyle by an expert. Children in need of one specific type of healthcare for deprived children were: moderately deprived. Children in need of referral to a socio-medical unit for access to healthcare were: severely deprived. The main outcome measure was the agreement between FrenChILD-Index results and expert evaluation.Development phases produced a 12-item instrument. Validation phases were carried out in a 986 children sample. FrenChILD-Index fulfilled the Terwee validity criterion for screening instruments. For moderate deprivation, sensitivity was 96.0% [92.6; 98.7] and specificity 68.3% [65.2; 71.4]. For severe deprivation, sensitivity was 96.3% [92.7; 100] and specificity 91.1% [89.2; 92.9]. It correlated with the number of lifestyle deprived domains 0.80 [0.77; 0.83] and the amount of specific healthcare for children 0.86 [0.83; 0.88].Conclusions: FrenChILD-Index is the first pediatric individual-level index of deprivation, methodologically validated in Europe. FrenChILD-Index enables individual appropriate referral for deprived children. It enables considering social determinants of health into account in epidemiological adjustment, patient sample stratification and program impact measurement. (NCT03640715, 21/08/2018)
Trajectories of Prenatal Alcohol Consumption in An Australian Population-Based Cohort of Pregnant Women - A Data Driven Approach
Authors: Muggli Evelyne , Hearps Stephen , Halliday Jane , Elliott Elizabeth J , Penington Anthony , Thompson Deanne K , Spittle Alicia , Forster Della A , Lewis Sharon , Anderson Peter J 
Published: August 2021

Abstract
Accurate information on dose, frequency and timing of maternal alcohol consumption is critically important when investigating fetal risks from prenatal alcohol exposure. Identification of distinct alcohol use behaviours can also assist in developing directed public health interventions to prevent adverse child outcomes, including Fetal Alcohol Spectrum Disorder. We aimed to determine group-based trajectories of time-specific, unit-level, alcohol consumption using data from 1458 pregnant women in the Asking Questions about Alcohol in Pregnancy (AQUA) longitudinal study in Melbourne, Australia. Six alcohol consumption trajectories were identified incorporating four timepoints across gestation. Labels were assigned based on consumption in trimester one and whether alcohol use was continued throughout pregnancy: abstained (33.8%); low discontinued (trimester one) (14.4%); moderate discontinued (11.7%); low sustained (13.0%); moderate sustained (23.5%); and high sustained (3.6%). Median weekly consumption in trimester one ranged from 3 grams (low discontinued) to 184 grams of absolute alcohol (high sustained). Alcohol use after pregnancy recognition decreased dramatically for all sustained drinking trajectories, indicating some awareness of fetal risk. Improved understanding of factors that contribute to alcohol consumption in pregnancy in specific sub-populations is critical when developing prevention strategies. Maternal characteristics associated with different trajectories can inform the strategic development of such initiatives.
The Effect of Rebamipide Administration to the Improvement of Neutrophil-lymphocyte Ratio in COVID-19 Confirmed Patients with ARDS
Authors: Suryantoro Satriyo Dwi , Veterini Anna Surgean , Mahdi Bagus Aulia 
Published: August 2021

Abstract
Rebamipide has a noteworthy potential as an adjunct therapy of coronavirus disease (COVID-19) which may act as an anti- IL8. There have been no yet previous studies declared worldwide examining the use of it. We conducted a study of Rebamipide through the secondary data obtained from the medical record then analyzed the neutrophile, lymphocytes, and neutrophil-lymphocyte ratio of patients. Our data exhibited improvements in the laboratory outcomes, revealing decreased neutrophils, increased lymphocytes, also increased neutrophil-lymphocyte rations, observed in COVID-19 patients experiencing acute respiratory distress syndrome (ARDS).
Cisplatin Increased the Expression of PD-1 and PD-L1 by YAP1 in Hepatocellular Carcinoma
Authors: Hao Liyuan , Guo Yinglin , Peng Qing , Zhang Zhiqin , Li Shenghao , Ji Jingmin , Xue Yu , Liu Yiwei , Li Caige , Shi Xinli 
Published: August 2021

Abstract
Hepatocellular carcinoma (HCC) was one of the most malignant cancers in the world. Cisplatin (DDP) was one of the main chemotherapy drugs for HCC, but the mechanism of DDP treatment for HCC remains unclear. In this presentation, we found that DDP inhibited the growth of HCC cells and promoted the expression of PD-1 and its ligand PD-L1 in cancer cells. Meanwhile, flow cytometry analysis revealed that DDP enhanced PD-1-CD8+ T cells expression and decreased PD-1+CD8+ T cells expression. ELISA analysis suggested that DDP decreased TGF-β expression in vivo. Therefore, the study indicated that DDP enhanced PD-1 and PD-L1 expression and inhibited the growth of HCC.
Low CCL19 Expression Is Associated With Adverse Clinical Outcomes For Patients With Advanced Stage Follicular Lymphoma
Authors: Zhou Yu , Wang Shasha , Tao Yunxia , Chen Haizhu , Qin Yan , He Xiaohui , Zhou Shengyu , Liu Peng , Yang Jianliang , Yang Sheng , Gui Lin , Lou Ning , Zhang Zhishang , Yao Jiarui , Han Xiaohong , Shi Yuankai 
Published: August 2021

Abstract
Background: This study aimed to explore the interactions and relationships of genes and recognize the hub genes associated with prognosis in follicular lymphoma (FL) treated with first-line rituximab combined with chemotherapy.Method: RNA sequencing data of dataset GSE65135 (n=24) were included in differentially expressed genes (DEGs) analysis. Weighted gene co-expression network analysis (WGCNA) was applied for exploring the coexpression network and identifying hub genes. Validation of hub genes expression and prognosis were applied in dataset GSE119214 (n=137) and patient cohort of Cancer Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College (n=32), respectively, by analyzing RNAseq expression data and serum protein concentration quantified by ELISA. CIBERSORT was applied for tumor-infiltrating immune cells (TIICs) subset analysis. Results: A total of 3260 DEGs were obtained, with 1861 genes upregulated and 1399 genes downregulated. Using WGCNA and Cytoscape analysis, eight hub genes, PLA2G2D, MMP9, PTGDS, CCL19, NFIB, YAP1, RGL1, and TIMP3 were identified. Kaplan-Meier analysis and multivariate COX regression analysis indicated that CCL19 independently associated with overall survival (OS) for FL patients treated with rituximab and chemotherapy (HR = 0.47, 95%CI [0.25-0.86], p = 0.014). Higher Serum CCL19 concentration was associated with longer progression-free survival (PFS, p=0.014) and OS (p=0.039). TIICs subset analysis showed that CCL19 expression had a positive correlation with monocytes and macrophages M1, and a negative correlation with naïve B cells and plasma cells. Conclusion: CCL19 expression was associated with survival outcomes and might be a potential prognostic biomarker for FL treated with first-line immunochemotherapy.
A Small Molecule Modulating Monounsaturated Fatty Acids and Wnt Signaling Confers Maintenance to Induced Pluripotent Stem Cells Against Endodermal Differentiation
Authors: Hosseini Vahid , Kalantary-Charvadeh Ashkan , Hajikarami Maryam , Fayyazpour Parisa , Rahbarghazi Reza , Totonchi Mehdi , Darabi Masoud 
Published: August 2021

Abstract
Background: Stearoyl-coenzyme A desaturase 1 (SCD1) is required for de novo synthesis of fatty acids. This enzyme can orchestrate posttranslational modification of proteins involved in the development and differentiation of cells through the fatty acid acylation process. In this study, we evaluated whether a small molecule modulating unsaturated fatty acids influences early endodermal differentiation of induced pluripotent stem cells, using biochemical methods and immunostaining.Methods: The hiPSCs were cultured in an endoderm-inducing medium containing activin A and low defined fetal bovine serum in the presence of an SCD1 inhibitor at different time points. The yield of three germ layers endoderm, mesoderm, and ectoderm, and the cell cycle analysis were assessed using flow cytometry. The expression of endoderm and pluripotency markers, as well as the expression of Wnt signaling pathway proteins, were assessed using western blotting and RT-PCR. Total protein acylation was evaluated using a click chemistry reaction.Results: The population of cells showing endoderm features was decreased at the end of differentiation when SCD1 was inhibited on the first day. Moreover, early SCD1 inhibition preserved hiPSCs properties without a shift toward mesoderm or ectoderm. Treatment of cells with SCD1 inhibitor only on the first day decreased the β-catenin gene expression and intensity of fluorescent emission in the click chemistry. These effects were effectively rescued by cotreatment with oleate. Late treatment at two subsequent days of endoderm induction induced no significant effect on endoderm-specific markers and fluorescent intensity. Reproducible results were also obtained with a human embryonic stem cell line. Conclusion: The small molecule SCD1 inhibitor attenuates the Wnt/β-catenin signaling pathway, conferring maintenance to hiPSCs by opposing the initiation of endoderm differentiation. The immediate requirement for SCD1 activity in endoderm commitment of pluripotent stem cells may be eminent in disorders of endoderm-derived organs and dysregulated metabolism.
Dietary Intakes, Knowledge and Perceptions of Semi-Professional Rugby Athletes in Scotland.
Authors: Hitendre Sonam , Jordan Rebecca , Theodorakopoulos Chistos , White Lois 
Published: August 2021

Abstract
Background
Adequate nutritional intake plays a pivotal role in optimising performance, recovery and body composition goals. This study aimed to investigate the dietary intakes (DI), nutritional knowledge (NK) and attitudes, perceptions and challenges (APC) of semi-professional rugby players in Scotland.
Methods
Dietary intakes and NK of 24 male semi-professional rugby players of a Super6 club were evaluated using validated questionnaires. Players were categorized as having good or poor NK according to NK scores. Diet-related APCs were assessed online using researcher developed questionnaires and 1–1 semi-structured interviews.
Results
Mean ± SD total NK% was poor, 53.7 ± 11.9%. The ‘Good’ NK group scored significantly higher in the Weight Management (p = 0.014), Macronutrients (p < 0.001), Micronutrients (p = 0.001) and Sports Nutrition (p < 0.001) sections. Mean DI were 26.3 ± 9.2 kcal/kg/day energy, 1.4 ± 0.4 g/kg/day protein, 21.7 ± 10.1 g/day fibre. Median (25th ,75th ) carbohydrate intake was 3.0 (2.0,3.0) g/kg/day and 6.3 (2.3,10.6) units/week alcohol. Mean ± SD fat and saturated fat (SFA) % total energy intake (EI) were 36.2 ± 3.7% and 12.8 ± 1.9% respectively, and SFA %EI exceeded recommendations (p < 0.001). The ‘Good’ NK group had a significantly higher intakes of all macronutrients (p < 0.05). Total NK% positively correlated with intakes of meat (r = 0.556, p = 0.011), cereals (r = 0.458, p = 0.042), dietary fat (r = 0.477, p = 0.034), vegetables (r = 0.487, p = 0.030) and alcoholic beverages (r = 0.541, p = 0.014). Supplement use was 68%. Players felt diet affected performance (94%) but 31% of them were unaware of any specific nutritional strategies. A healthy diet was perceived to be ‘balanced’ with ‘variety from all food groups’. Lack of time for preparation was described as the main barrier to healthy eating.
Conclusions
Overall players had poor NK, their fibre and carbohydrate intake was suboptimal, whereas saturated fat intake exceeded recommendations. Many lacked awareness of current sports nutrition guidelines. Further nutrition education may be needed to improve diet quality and aid performance goals.
Contribution of IL-38 in Lung Immunity During Pseudomonas Aeruginosa-Induced Pneumonia
Authors: Wei Qiang , Chen Xi , Wang Chuanjiang 
Published: August 2021

Abstract

Objective: Interleukin-38 (IL-38), a new type of cytokine, is involved in processes such as tissue repair, inflammatory response, and immune response. However, its function in pneumonia caused by Pseudomonas aeruginosa is still unclear.Methods: In this study, we detected circulating IL-38 in adults affected by pneumonia caused by P. aeruginosa. The P. aeruginosa-induced pneumonia WT murine model was adopted to evaluate the effect of IL-38 on Treg differentiation, cell apoptosis, survival, tissue damage, inflammation, and bacterial removal.Results: IL-38 is insufficiently secreted in patients who died of P.A. pneumonia.Recombinant IL-38 improved survival, whereas anti-IL-38 antibody reduced survival in the experimental pneumonia murine model. IL-38 exposure reduced the inflammatory response, as suggested by the lung injury, and reduced cytokine levels (IL-1β, IL-6, IL-17A, TNF-α, and CXCL-1, but not IL-10). It also increased bacterial clearance and reduced cell apoptosis in the lungs. Furthermore, IL-38 was shown to reduce TBK1 expression in vitro when naïve CD4+ T lymphocytes were differentiated to Tregs and played a protective role in P.A. pneumonia.Conclusions: To summarize, the above findings provide additional insights into the mechanism of IL-38 in the treatment of P.A. pneumonia.
Schisandrin A protects intestinal cells from mycophenolic acid-induced cytotoxicity and oxidative damage
Authors: Deng Yiyun , Zhang Zhe , Hong Yuanyuan , Feng Lijuan , Su Yong , Xu Dujuan 
Published: August 2021

Abstract
Objectives: The gastrointestinal side effects of mycophenolic acid affect its efficacy in kidney transplant patients, which may be due to its toxicity to the intestinal epithelial mechanical barrier, including intestinal epithelial cell apoptosis and destruction of tight junctions. The toxicity mechanism of mycophenolic acid is related to oxidative stress-mediated the activation of mitogen-activated protein kinases (MAP K). Schisandrin A (Sch A), one of the main active components of the Schisandra chinensis, can protects intestinal epithelial cells from deoxynivalenol-induced cytotoxicity and oxidative damage by antioxidant effects. The aim of this study was to investigate the protective effect and potential mechanism of Sch A on mycophenolic acid-induced damage in intestinal epithelial cell. Methods: Caco-2 cells monolayers were treated with mycophenolic acid (10µM) and/or Sch A (10, 20 and 40µM) at 37°C for 24h, and cell viability was measured by MTT; Western blot and immunofluorescence were used to detect the expression of relevant proteins. Intracellular ROS and apoptosis were measured by flow cytometry, and malondialdehyde (MDA) and superoxide dismutase (SOD) levels were measured by kits. Results: The results showed that Sch A significantly reversed the mycophenolic acid-induced cell viability reduction, restored the expression of tight junction protein ZO-1, occludin and reduced cell apoptosis. In addition, Sch A inhibited mycophenolic acid-mediated MAPK activation and reactive oxygen species (ROS) increase. Conclusions: Sch A protected intestinal epithelial cells from mycophenolic acid intestinal toxicity, at least in part, by reducing oxidative stress and inhibiting MAPK signaling pathway. Conclusions: Sch A protected intestinal epithelial cells from mycophenolic acid intestinal toxicity, at least in part, by reducing oxidative stress and inhibiting MAPK signaling pathway.
Spinal Hemangioblastomas: Analysis of Surgical Outcome and Prognostic Factors
Authors: Feletti Alberto , Boaro Alessandro , Giampiccolo Davide , Casoli Giorgio , Moscolo Fabio , Ferrara Massimiliano , Sala Francesco , Pavesi Giacomo 
Published: August 2021

Abstract
Background. The prognostic factors for surgically removed spinal hemangioblastomas, the impact of VHL disease on outcome, and the role of intraoperative neuromonitoring are still not completely clear. The aim of this study was to review our experience with spinal hemangioblastomas in order to assess potential predictors of neurological outcome after surgery.Methods. All cases of spinal hemangioblastomas removed at two Italian academic institutions from 1985 to 2020 were reviewed. Data about clinical presentation and symptoms duration, diagnosis of VHL, surgical approach, use of IONM, duration of hospital stay, follow up, McCormick grade before and after surgery were extracted.Results. Sixty-one patients (31 F, 30 M) underwent 69 surgeries to remove 74 spinal hemangioblastomas (37 cervical, 32 thoracic, 5 lumbar). Improvement was found in 32.3% of cases, neurological condition remained stable in 51.6% of cases, and deteriorated in 16.1% of patients.A worsening trend in VHL patients and an improvement trend in non-VHL patients were detected, despite the lack of statistical significance. Laminotomy and use of IONM were found to be associated with better outcome, although no association was found between surgery without IOM and worse outcome.Conclusion. In most cases, patients affected by spinal hemangioblastomas can expect a good long-term outcome. In our experience, laminotomy seems to be associated with better outcome compared to laminectomy. While its absence is not associated with worse outcome, IONM seems to be associated with a better neurological outcome. Our study suggests that the more impaired the preoperative neurological condition, the worse the outcome.
Molecular Characterization of Distinct Fungal Communities in the Soil of a Rare Rarth Mining Area
Authors: Liu jingjing , Li Chun , Ma Wendan , Liu Wei , Wu Weixiang 
Published: August 2021

Abstract
The exploitation of ion absorbed rare earth elements (REEs) has caused serious ecological destruction and environmental pollution. Effect on soil fungal structure and diversity caused by mining activities are usually ignored, although fungi are one of the most important components in soil ecosystems. In the present research, quantitative polymerase chain reaction (qPCR) and high-throughput Illumina MiSeq sequencing were conducted to characterize fungal community composition and structure in soil of a rare earth mining area after in-situ leaching. Statistical analyses, Network and FUNGuild were used to conduct in-depth analysis. Ascomycota, Basidiomycota, Glomeromycota and unclassified fungi were the most abundant phyla in the mining soils. Organic matter, TC and TN contents, but not pH or REEs contents, were the vital factors to determine soil fungal abundances and diversities. Fungal community structures were stable after leaching practice, but nutrition contents significantly and positively contributed to fungal abundances and diversities. Fungi could mediate the interaction between species to enhance their ability to resist the harsh environment of REEs toxicity or ammonium caused by in-situ leaching practice. Saprotroph in phyla Ascomycota and Basidiomycota were the dominant fungal trophic mode in the mining soils, and they played a critical role in nutrient cycling, transformation processes and reducing metal toxicity. Symbiotrophs of phyla Glomeromycota contributed to soil aggregation and slowing down nutrient losses after in-situ leaching practice.