bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification of lncRNA–mRNA Regulatory Network Associated With Isolated Systolic Hypertension and Atherosclerotic Cerebral Infarction
Authors: Yang jie , Tao Yan-Nan , Hu Fang-Xiao , Chen Yong-Zhi , Yang Xue-Song , Zhuang Xin , Cui Ming-Ling , Li Guan-lan , Li Chao , Yang Chuan-Hua , Jiang Yue-hua 
Published: April 2021

Abstract
Background: Increasing evidences uncover that lncRNAs play an important role in Isolated systolic hypertension (ISH). However, a systematic lncRNA-mRNA regulatory network is still absent in isolated systolic hypertension and atherosclerotic cerebral infarction patients (ISH & ACI).Aim:This research aims to establish a lncRNA-mRNA co-expression network in patients with ISH & ACI, to probe into the potential functions of lncRNA in those patients.Design and Setting:Expression profiles of lncRNA and mRNAs are collected and compared respectively from 8 patients with ISH and 8 patients with ISH & ACI by RNA-seq data.Methods: Differentially expressed lncRNAs and mRNAs were screened out via high-throughput sequencing in the plasma of ISH/ACI patients and control ISH patients. Then, a lncRNA-mRNA interaction network was built using the Pearson correlation coefficient by Cytoscape software. The expression levels of the hub genes and lncRNAs were verified by qRT-PCR in another 10 ISH/ACI patients and 10 control patients. Results: 2768 differentially expressed lncRNAs and 747 differentially expressed mRNAs were identified. 2 hub genes (CD226 and PARVB) and 11 lncRNAs were identified in the lncRNA-mRNA interaction network. qRT-PCR and cell assay results verified that lncRNAs ENST00000590604 and CD226 are highly expressed in patients of ISH & ACI. CD226 was associated with vascular endothelial cells growth and stability through platelet activation and focal adhesion pathway.Conclusion: We established a novel mRNA-lncRNA interaction network. lncRNAs ENST00000590604 and CD226 might be the potential biomarkers of ISH & ACI.
Up-regulation thioredoxin contributes to inhibite diabetic hearing impairment
Authors: Ren Xiang , Lv Jinjuan , Fu Yuzhen , Zhang Na , Zhang Chenghong , Dong Zhenghao , Chudhary Maryam , Zhong Shiwen , Kong Li , Kong Hui 
Published: April 2021

Abstract
Diabetes mellitus (DM) is a metabolic disorder characterized by chronic hyperglycemia. An association between DM and hearing impairment has been widely discussed. It’s essential to find effective prevetion or diagnosis of diabetic hearing loss. Thioredoxin (Trx) is a small molecule protein (12kDa) and plays biological functions such as anti-apoptotic, transcriptional regulation. In this study, our aim is to clarify the protective effect of Trx on diabetic hearing loss to find the early potential therapeutic target of diabetic hearing impairment in clinic in the future. Trx transgenic(Tg) mice was used to induce diabetic model by intraperitoneal injected Streptozotocin (STZ) and with/without SF or PX12 treatment. Succinate Dehydrogenase (SDH) Staining was used to evaluate the loss of hair cells. The relative expression of related proteins and genes was detected by Western blot and qRT-PCR. In diabetic mice, the outer hair cells were lost significantly. However, the loss of hair cells was delayed over-expression Trx. Moreover, the expression of PGC-1α, bcl-2 and LC3 was increased in Tg(+)-DM mice compared with Tg(-)-DM mice. The expression of ASK1, Txnip, GRP78, CHOP and p62 was decreased in Tg(+)-DM mice compared with Tg(-)-DM mice. Taken together, up-regulation Trx can protect cochlear hair cell from damage in diabetes. The underlying mechanism may be related to regulate ER stress through ASK1 and mitochondria pathway or autophagy via Txnip.
Repeatability and Comparability of the Galilei-G4 and Cassini in Measuring Corneal Power and Astigmatism in Normal and Post-Refractive Surgery Eyes
Authors: Mohamed Mohamed , Khan Mahmood , Kanakamedala Amritha , Gupta Isha , Wang Li , Koch Douglas , Al-Mohtaseb Zaina 
Published: April 2021

Abstract
PURPOSE
To assess the repeatability and comparability of the Galilei G4 versus the Cassini topographer in post-refractive eyes and in normal eyes, including older patients representative of an initial cataract evaluation.
METHODS
Simulated keratometric (simK), total corneal and posterior corneal power and astigmatism were evaluated in both post-refractive and normal eyes. Repeatability was measured by calculating within-subject standard deviation (Sw), coefficient of variation (CoV), and intraclass correlation coefficient (ICC). Vector analyses and Bland-Altman plots were employed to assess agreement between devices.
RESULTS
We studied 32 subjects with a history of refractive surgery and 32 subjects without a history of refractive surgery undergoing cataract surgery. The mean age was 55 ± 18.5 years and the age range was 21.5 to 91.5 years. In non-refractive and post-refractive eyes, the ICC was > 0.9 (P < .001) for all corneal powers and for simK and total corneal astigmatism for both analyzers. The ICC for posterior corneal astigmatism magnitude using the Galilei was 0.62 and 0.67 and for the Cassini 0.55 and 0.38 in normal and post-refractive eyes, respectively.
CONCLUSIONS
In both post-refractive and normal eyes, the Galilei G4 and Cassini analyzers have high repeatability in simK, total, and posterior corneal power and low repeatability for posterior corneal astigmatism.
Role of Thrombospondin-1 in Sepsis-Induced Myocardial Injury
Authors: XIE YUN , ZHANG JIAXIANG , JIN WEI , TIAN RUI , Wang Ruilan 
Published: April 2021

Abstract
Objective: Sepsis often causes myocardial injury with a high mortality. We wanted to investigate the effects of thrombospondin-1 (THBS1) expression on myocardial cell injury, oxidative stress and apoptosis in sepsis.Methods: The expression of THBS1 mRNA in LPS-induced mouse primary cardiomyocytes was detected by real-time fluorescence quantitative PCR. We constructed a eukaryotic siRNA expression vector and used liposome transfection to knockdown THBS1 mRNA expression in myocardial cells. We detected the THBS1 mRNA expression level using real-time fluorescent quantitative PCR. Four groups were used: control, LPS, THBS1 siRNA, and LPS + THBS1 siRNA. ELISA was used to detect cTnI, proBNP, ROS, caspase3 and other indicators of cell damage. At the same time, sepsis mouse models were prepared for H&E, TUNEL and caspase-3 staining to evaluate myocardial cell injury and apoptosis. Clinical samples were collected to analyze the serum THBS1 level and correlate it with the prognosis of patients with myocardial injury of sepsis.Results: The expression level of THBS1 mRNA in myocardial cells induced by LPS was increased, and the serum THBS1 level in patients with myocardial injury in sepsis was also significantly increased. In the THBS1 siRNA group with myocardial injury, the levels of cTnI and proBNP were significantly decreased, the levels of the inflammatory cytokines IL-6 and TNF-α were significantly decreased, ROS were significantly decreased, and caspase3 was significantly decreased, and myocardial cell apoptosis was also reduced in the sepsis mouse model. Conclusion: THBS1 is closely related to the biological behavior of myocardial cells and may be a therapeutic target for myocardial injury in sepsis.
A Novel Ceruloplasmin Mutation Identified in a Chinese Patient and Clinical Spectrum of Aceruloplasminemia Patients
Authors: Xu Wan-Qing , Ni Wang , Wang Rou-Min , Dong Yi , Wu Zhi-ying 
Published: April 2021

Abstract
Background and Purpose: Aceruloplasminemia (ACP) is a rare disorder of iron overload resulting from ceruloplasmin (CP) variants. Because of its rarity and heterogeneity, the diagnosis of ACP is often missed or misdiagnosed. Here, we aim to present a clinical spectrum of ACP and raise more attention to the early diagnosis. Methods: Whole exome sequencing (WES) was performed in a Chinese female patient suspected with ACP and her clinical data were collected in detail. The PubMed databases was searched for published ACP patients within the last decade, and we present a systematic review of their clinical features with data extracted from these researches. Results: A novel pathogenic variant (c.2689delC) and a known pathogenic variant (c.606dupA) within ceruloplasmin gene were identified in our patient and confirmed the diagnosis of ACP. Then we reviewed 50 ACP patients including the case we reported here. A possible timeline of symptoms was discovered, anemia appears first (29.7 years old on average), followed by diabetes (37.3 years old) and finally neurological symptoms (52.2 years old). The delay in diagnosis was significantly shortened in patients without neurological symptoms. Biochemical triad including anemia, low to undetectable serum ceruloplasmin, low serum iron and/or hyperferritinemia, showed better sensitivity in diagnosis than clinical triad including diabetes, neurological symptoms and retinal degeneration. Conclusions: Due to the variable symptom spectrum, patients with ACP often visit different departments, which can lead to misdiagnosis. Clinical attention needs to be paid to symptoms and tests that have a warning effect. Prompt diagnosis in the early stage of the disease can be beneficial.
Effectiveness Comparisons of Drug Therapies for Postoperative Aneurysmal Subarachnoid Hemorrhage Patients: Network Meta‑analysis and systematic review
Authors: Yu Wanli , Huang Yizhou , Zhang Xiaolin , Luo Huirong , Chen Weifu , Jiang Yongxiang , Cheng Yuan 
Published: April 2021

Abstract
Objective: To compare the effectiveness of various drug interventions in improving the clinical outcome of postoperative patients after aneurysmal subarachnoid hemorrhage (aSAH) and assist in determining the drugs of definite curative effect in improving clinical prognosis.Methods: Eligible Randomized Controlled Trials (RCTs) were searched in databases of PubMed, EMBASE, and Cochrane Library (inception to Sep 2020). Glasgow Outcome Scale (GOS) score, Extended Glasgow Outcome Scale (GOSE) score or modified Rankin Scale (mRS) score was used as the main outcome measurements to evaluate the efficacy of various drugs in improving the clinical outcomes of postoperative patients with aSAH. The network meta-analysis (NMA) was conducted based on a random-effects model, dichotomous variables were determined by using odds ratio (OR) with 95% confidence interval (CI), and a surface under the cumulative ranking curve (SUCRA) was generated to estimate the ranking probability of comparative effectiveness among different drug therapies.Results: From the 493 of initial citation screening, forty-four RCTs (n=10626 participants) were eventually included in our analysis. Our NMA results showed that cilostazol (OR=3.35,95%CI=1.50,7.51) was the best intervention to improve the clinical outcome of patients (SUCRA=87.29%, 95%CrI 0.07-0.46). Compared with the placebo group, only two drug interventions [nimodipine (OR=1.61, 95%CI 1.01,2.57) and cilostazol (OR=3.35, 95%CI 1.50, 7.51)] achieved significant statistical significance in improving the clinical outcome of patients. Conclusions: Both nimodipine and cilostazol have exact curative effect to improve the outcome of postoperative patients with aSAH, and cilostazol may be the best drug to improve the outcome of patients after aSAH operation. Our study provides implications for future studies that, the combination of two or more drugs with relative safety and potential benefits (e.g., nimodipine and cilostazol) may improve the clinical outcome of patients more effectively.
Behavioural responses to Covid-19 health certification: A rapid review
Authors: Drury John , Mao Guanlan , John Ann , Kamal Atiya , Rubin G James , Stott Clifford , Vandrevala Tushna , Marteau Theresa M 
Published: April 2021

Abstract
Background Covid-status certification – certificates for those who test negative for the SARS-CoV-2 virus, test positive for antibodies, or who have been vaccinated against SARS-CoV-2 – has been proposed to enable safer access to a range of activities. Realising these benefits will depend in part upon the behavioural and social impacts of certification. The aim of this rapid review was to describe public attitudes towards certification, and its possible impact on uptake of testing and vaccination, protective behaviours, and crime.Method A search was undertaken in peer-reviewed databases, pre-print databases, and the grey literature, from 2000 to December 2020. Studies were included if they measured attitudes towards or behavioural consequences of health certificates based on one of three indices of Covid-19 status: test-negative result for current infectiousness, test-positive for antibodies conferring natural immunity, or vaccination(s) conferring immunity. Results Thirty-three papers met the inclusion criteria, only three of which were rated as low risk of bias. Public attitudes were generally favourable towards the use of immunity certificates for international travel, but unfavourable towards their use for access to work and other activities. A significant minority was strongly opposed to the use of certificates of immunity for any purpose. The limited evidence suggested that intention to get vaccinated varied with the activity enabled by certification or vaccination (e.g., international travel). Where vaccination is seen as compulsory this could lead to unwillingness to accept a subsequent vaccination. There was some evidence that restricting access to settings and activities to those with antibody test certificates may lead to deliberate exposure to infection in a minority. Behaviours that reduce transmission may decrease upon health certificates based on any of the three indices of Covid-19 status, including physical distancing and handwashing.Conclusions The limited evidence suggests that health certification in relation to COVID-19 – outside of the context of international travel – has the potential for harm as well as benefit. Realising the benefits while minimising the harms will require real-time evaluations allowing modifications to maximise the potential contribution of certification to enable safer access to a range of activities.
Pelargonidin as an Attenuator of Neuronal Mitochondrial Dysfunction: An in Vivo Study
Authors: Rashed Engy , El-Hamoly Tarek , El-Sheikh Marwa , El-Ghazaly Mona A. 
Published: April 2021

Abstract
Targeting the neuronal mitochondria as a possible intervention to guard against neurodegenerative disorders' progression has been investigated in the current work via the administration of Pelargonidin (PEL) to rats intoxicated by the mitochondrial toxin Reserpine. The main criteria for choosing pelargonidin (PEL) were its reported poly (ADP-ribose) polymerase (PARP)-inhibitor, antioxidant, anti-apoptotic and anti-inflammatory activities. Male rats were randomized into four experimental groups; normal control, reserpinized to induce mitochondrial failure, standard PARP-1-inhibitor 1,5-isoquinolinediol (DIQ)-treated reserpinized, and PEL-treated reserpinized . PEL significantly restored brain glutathione (GSH) with a reduction in nitric oxide contents as compared to reserpine-challenged group. Meanwhile, it improved the neuronal mitochondrial function by the elevation of complex I activity associated with a low ADP/ATP ratio. Likely, through its anti-inflammatory effect, PEL reduced the elevation of serum interlukine-1ß level and inhibited serum lactate dehydrogenase (LDH) activity. These findings were aligned with the reduced expressions of cleaved PARP and cleaved caspase-3 proteins, indicating PEL suppressive effect to the intrinsic apoptotic pathway. Those biochemical findings were confirmed through comparable histopathological tissue examination among the experimental groups. In conclusion, PEL is a promising candidate for the future use in management of mitochondria-associated neuronal complications via controlling the ongoing inflammatory and degeneration cascades.
Effects of Mesh Loop Modes on Performance of Unstructured Finite Volume GPU Simulations
Authors: Weng Yue , Zhang Xi , Guo Xiaohu , Zhang Xianwei , Lu Yutong , Liu Yang 
Published: April 2021

Abstract
In unstructured finite volume method, loop on different mesh components such as cells, faces, nodes, etc is used widely for the traversal of data. Mesh loop results in direct or indirect data access that affects data locality significantly. By loop on mesh, many threads accessing the same data lead to data dependence. Both data locality and data dependence play an important part in the performance of GPU simulations. For optimizing a GPU-accelerated unstructured finite volume Computational Fluid Dynamics (CFD) program, the performance of hot spots under different loops on cells, faces, and nodes is evaluated on Nvidia Tesla V100 and K80. Numerical tests under different mesh scales show that the effects of mesh loop modes are different on data locality and data dependence. Specifically, face loop makes the best data locality, so long as access to face data exists in kernels. Cell loop brings the smallest overheads due to non-coalescing data access, when both cell and node data are used in computing without face data. Cell loop owns the best performance in the condition that only indirect access of cell data exists in kernels. Atomic operations reduced the performance of kernels largely in K80, which is not obvious on V100. With the suitable mesh loop mode in all kernels, the overall performance of GPU simulations can be increased by 15%-20%. Finally, the program on a single GPU V100 can achieve 4.8 speed up comparing with 28 MPI tasks on two Intel CPUs Xeon Gold 6132.
Does Anterior Controllable Antidisplacement and Fusion Technique Improve Quality of Life in Revision Treatment for Cervical Ossification of the Posterior Longitudinal Ligament? Three-Year Follow-Up Study
Authors: Li Hai-Dong , Min Ji-Kang 
Published: April 2021

Abstract
Background: The laminoplasty and laminectomy are two comparatively safe posterior procedures for the treatment of OPLL. The long-term outcomes of posterior surgery seem to be favorable, however it is also criticized for C5 nerve root palsy, progression OPLL and bad cervical lordosis. An additional revision surgery was required for those patients who had the poor clinical improvement after the initial posterior surgery.Methods: This study was to identify the mid-term clinical results and the health-related quality of life (HRQoL) after anterior controllable antidisplacement and fusion (ACAF) technique for revision treatment of cervical ossification of the posterior longitudinal ligament (OPLL). All the patients were followed up for more than 3 years. The Japanese Orthopaedic Association (JOA) scales, Neck Disability Index (NDI) and Short-Form 36 (SF-36) were recorded to evaluate the health-related quality of life. And the neck and arm pain scale as an expression of visual analog scale (VAS) were also collected. Intraoperative parameters, clinical features, radiological investigations and the surgery-related complications were performed to analysis. Results: There were 20 patients who underwent ACAF revision surgery between March 2017 and January 2019. Clinical improvements were observed in all outcomes; significant improvements on the NDI, VAS, SF-36, and JOA scores were maintained at 3 years (P < 0.05). All the patients showed a better cervical alignment, and the average cervical lordosis was 17±4.6° after revision surgery. There was only one case of cerebrospinal fluid (CSF) leakage, no instance of C5 root platy, postoperative hematoma or esophageal injury occured. No instrumented failure or pseudarthrosis case was observed during the follow-up.Conclusions: This study suggested that ACAF is an effective and safe procedure for the revision treatment of cervical OPLL. Such a surgical technique can offer the postoperative improvement on pain levels and health-related quality of life.
A Randomized Controlled Trial to Evaluate and Assess the Effect of Comprehensive Pre-End Stage Kidney Disease Education on Home Dialysis Use in Veterans, Rationale and Design
Authors: Shukla Ashutosh M. , Hale-Gallardo Jennifer , Orozco Tatiana , Freytes Ivette , Romero Sergio , Jia Huanguang , Purvis Zachary 
Published: April 2021

Abstract
Background Informed dialysis selection and greater home dialysis use are the two long-desired, underachieved targets of advanced chronic kidney disease care in the US healthcare system. Observational institutional studies have shown that comprehensive pre-end stage kidney disease (ESKD) disease education (CPE) can improve both these outcomes. However, lack of validated protocols, well-controlled studies, and systemic models have limited wide-spread adoption of CPE in the US. We hypothesized that a universal CPE and patient-centered initiation of renal replacement therapy can improve multiple clinical, patient-centered and health service outcomes in advanced chronic kidney disease (CKD) and ESKD.Methods Trial to Evaluate and Assess the effects of CPE on Home dialysis in Veterans (TEACH-VET) is a mixed method randomized controlled trial aimed to evaluate the effects of a system-based approach for providing CPE to all Veterans with advanced CKD across a regional healthcare System. The study will randomize 544 Veterans with non-dialysis stage 4 and 5 CKD in a 1:1 allocation stratified by their annual family income and the stage of CKD to an intervention (CPE) arm or control arm. Intervention arm will receive a two-phase CPE in an intent-to-teach manner. Control arm will receive usual clinical care supplemented by resources for the freely-available kidney disease information. Participants will be followed after intervention/control for the duration of the study or until 90-days post-ESKD, whichever occurs earlier.Results The primary outcome will assess the proportion of Veterans using home dialysis at 90-days post-ESKD, and secondary outcomes will include post-intervention/control CKD knowledge, confidence in dialysis decision and home dialysis selection. Qualitative arm of the study will use semi-structured interviews to in-depth assess Veterans’ satisfaction with the intervention, preference for delivery, and barriers and facilitators to home dialysis selection and use. Several post-ESKD clinical, patient-centered and health services outcomes will be assessed 90-days post-ESKD as additional secondary outcomes.Conclusion The results will provide evidence regarding the need and efficacy of a system-based, patient-centered approach towards universal CPE for all patients with advanced CKD. If successful, this may provide a blueprint for developing such programs across the similar healthcare infrastructures throughout the country.Trial registration: NCT04064086