bluuebunny/crossref_metadata_2025_split huggingface dataset:
Metabolic potentials of members of the class Acidobacteriia in metal‐contaminated soils revealed by metagenomic analysis
Authors: Xu Rui , Sun Xiaoxu , Häggblom Max M. , Dong Yiran , Zhang Miaomiao , Yang Zhaohui , Xiao Enzong , Xiao Tangfu , Gao Pin , Li Baoqin , Sun Weimin 
Published: June 2021

SummaryThe relative abundance of Acidobacteriia correlated positively with the concentrations of arsenic (As), mercury (Hg), chromium (Cr), copper (Cu) and other metals, suggesting their adaptation of the metal‐rich environments. Metagenomic binning reconstructed 29 high‐quality metagenome‐assembled genomes (MAGs) associated with Acidobacteriia, providing an opportunity to study their metabolic potentials. These MAGs contained genes to transform As, Hg and Cr through oxidation, reduction, efflux and demethylation, suggesting the potential of Acidobacteriia to transform such metal(loid)s. Additionally, genes associated with alleviation of acidic and metal stress were also detected in these MAGs. Acidobacteriia may have the capabilities to resist or transform metal(loid)s in acidic metal‐contaminated sites. Moreover, these genes encoding metal transformation could be also identified in the Acidobacteriia‐associated MAGs from five additional metal‐contaminated sites across Southwest China, as well as Acidobacteriia‐associated reference genomes from the NCBI database, suggesting that the capability of metal transformation may be widespread among Acidobacteriia members. This discovery provides an understanding of metabolic potentials of the Acidobacteriia in acidic metal‐rich sites.
Dual‐fluorescence imaging and automated trophallaxis detection for studying multi‐nutrient regulation in superorganisms
Authors: Baltiansky Lior , Sarafian‐Tamam Einav , Greenwald Efrat , Feinerman Ofer 
Published: June 2021

Abstract

Much like a single organism, colonies of social insects regulate their collective nutrition towards a specific intake of nutrients. Yet, the mechanisms behind this colony‐level regulation are not fully understood. Although foraging behaviour in social insects has been studied extensively, not much is known on its relation with feeding processes that occur within the nest. In the nest, food is commonly transferred between individuals in numerous oral feeding interactions (trophallaxis). Studies on the properties of these trophallactic networks have so far been limited by: (a) the difficulty to non‐intrusively measure food inside individual ants, let alone its nutritional composition, and (b) the meticulous manual labour involved in detecting trophallactic events.

Our dual‐fluorescence imaging set‐up is designed to track two food sources, each labelled with a different fluorophore, as they are disseminated throughout a freely behaving colony of individually tagged ants. Additionally, our image‐based deep learning algorithm for automatic detection of ant trophallaxis events efficiently yields a detailed record of all food‐transfer interactions.

Using a series of calibration experiments, we demonstrate the reliability of our measurements. We then exemplify the capabilities of our new method by tracking food dissemination in a colony of Camponotus sanctus ants supplied with two nutritionally distinct food sources. We follow the amount of each food type in the crop of each ant in the colony. Our data reveal the path in nutrient space that the colony took to reach its final nutrient intake, the contributions of the different forager ants to this path and the dynamic nutritional states of all ants in the colony. We further demonstrate the potential applicability of dual‐fluorescence food imaging to other insect species.

Our system provides access to data that were previously unavailable and is crucial for a complete description of nutrient regulation by social insects. More broadly, the ability to simultaneously and efficiently track two material flows in a behaving colony provides new opportunities for tackling many open and emerging questions on topics other than nutrition, such as disease spread and social regulation of colony development.
Dioscin elevates lncRNA MANTIS in therapeutic angiogenesis for heart diseases
Authors: Kong Chuiyu , Lyu Dayin , He Chang , Li Rui , Lu Qiulun 
Published: June 2021

AbstractDioscin has been widely used in clinics for coronary artery disease (CAD) treatment for years in China. However, the underlying mechanism for Dioscin‐mediated cardioprotective effect has not been elucidated. Here, we showed that Dioscin significantly rescues the cardiac function in mouse model of myocardial infarction (MI), accompanied by the reduction of cardiac fibrosis and apoptosis, resulting from elevated angiogenesis. Mechanistically, Dioscin promotes the proliferation and migration of hypoxic endothelial cells via the up‐regulation of lncRNA MANTIS, which serves as a scaffolding lncRNA within a chromatin remodeling complex. Meanwhile, it enables pol II binding to the transcription start sites, which leads to induced expression of angiogenesis‐related genes, including SOX18, SMAD6, and COUP‐TFII. Conversely, IncRNA MANTIS silencing prevents Dioscin‐induced migration and angiogenesis in hypoxic endothelial cells. Taken together, these data provide new insights that clarifies the cardioprotective effects of Dioscin against myocardial infarcted injury and confirms the effect on angiogenic activity of endothelial cells. This will build a solid theoretical basis for clinical therapeutic strategies.
Guideline adherence and clinical relevance of laboratory investigations during follow‐up in paediatric coeliac disease: A Dutch single‐centre cohort study
Authors: van Kalleveen Michael W. , Dykstra Tim C. , de Meij Tim G. J. , Plötz Frans B. 
Published: June 2021

AbstractAimDutch national guidelines on follow‐up of paediatric celiac disease (CD) are available. The primary aim was to evaluate guideline adherence by paediatricians during follow‐up. The secondary aim was to determine the clinical relevance and diagnostic yield of routine laboratory tests suggested by these guidelines.MethodsA retrospective, single‐centre, cohort study was performed in paediatric CD patients who visited Tergooi Hospital, the Netherlands, between January 2017 and December 2019, with follow‐up of at least twelve months after diagnosis. We analysed guideline adherence, number of outpatient visits and all laboratory data.ResultsWe included 91 CD children with a median follow‐up of 4.0 years (range 1–16 years) and 162 follow‐up visits. Strict adherence amongst paediatricians during follow‐up was 8.0% (13/162 cases). A total of 1570 laboratory tests were performed of which 45.4% (713/1570) was in strict compliance with the Dutch national guidelines. Clinically relevant deviations were observed in 5.3% of requested laboratory tests.ConclusionStrict guideless adherence amongst paediatricians in follow‐up of paediatric CD was low and the clinical relevance of the suggested routine laboratory tests is limited. This underlines the increasing notion that evidence‐based guidelines on follow‐up of CD are warranted.
Dual commitment profiles and job satisfaction among temporary agency workers
Authors: Menatta Pietro , Consiglio Chiara , Borgogni Laura , Moschera Luigi 
Published: June 2021

AbstractTemporary agency workers (TAWs) are an ever‐increasing type of employees which establish a double work relationship with both the agency and the client organization. Within this context, the concept of dual commitment has received considerable attention in the last years. The present contribution integrates dual commitment line of research with the one adopting a person‐centered approach to the study of commitment configurations, to investigate commitment profiles on a large sample of TAWs. According to Sinclair et al.'s framework, we aimed to identify TAWs' commitment profiles based on their levels of dual affective commitment (to the agency and to the client organization) and on their general continuance commitment and to investigate differences in job satisfaction among profiles. Latent profile analyses on 7225 TAWs revealed 5 distinct profiles, namely Dually Free Agents, Dually Involved, Dually Allied, (Unilaterally) Client Allied and (Unilaterally) Agency Invested. The Dually Involved profile, followed by the Dually Allied profile, had the highest level of job satisfaction, whereas the Dually Free Agent profile and the (Unilaterally) Agency Invested had the lowest. Furthermore, the (Unilaterally) Client Allied group had a higher level of job satisfaction as compared to the (Unilaterally) Agency Invested profile. Implications are discussed.
Generation of combined hepatocellular‐cholangiocarcinoma through transdifferentiation and dedifferentiation in p53‐knockout mice
Authors: Liu Yang , Xin Bing , Yamamoto Masahiro , Goto Masanori , Ooshio Takako , Kamikokura Yuki , Tanaka Hiroki , Meng Lingtong , Okada Yoko , Mizukami Yusuke , Nishikawa Yuji 
Published: June 2021

AbstractThe two principal histological types of primary liver cancers, hepatocellular carcinoma (HCC) and cholangiocarcinoma, can coexist within a tumor, comprising combined hepatocellular‐cholangiocarcinoma (cHCC‐CCA). Although the possible involvement of liver stem/progenitor cells has been proposed for the pathogenesis of cHCC‐CCA, the cells might originate from transformed hepatocytes that undergo ductular transdifferentiation or dedifferentiation. We previously demonstrated that concomitant introduction of mutant HRASV12 (HRAS) and Myc into mouse hepatocytes induced dedifferentiated tumors that expressed fetal/neonatal liver genes and proteins. Here, we examine whether the phenotype of HRAS‐ or HRAS/Myc‐induced tumors might be affected by the disruption of the Trp53 gene, which has been shown to induce biliary differentiation in mouse liver tumors. Hepatocyte‐derived liver tumors were induced in heterozygous and homozygous p53‐knockout (KO) mice by hydrodynamic tail vein injection of HRAS‐ or Myc‐containing transposon cassette plasmids, which were modified by deleting loxP sites, with a transposase‐expressing plasmid. The HRAS‐induced and HRAS/Myc‐induced tumors in the wild‐type mice demonstrated histological features of HCC, whereas the phenotype of the tumors generated in the p53‐KO mice was consistent with cHCC‐CCA. The expression of fetal/neonatal liver proteins, including delta‐like 1, was detected in the HRAS/Myc‐induced but not in the HRAS‐induced cHCC‐CCA tissues. The dedifferentiation in the HRAS/Myc‐induced tumors was more marked in the homozygous p53‐KO mice than in the heterozygous p53‐KO mice and was associated with activation of Myc and YAP and suppression of ERK phosphorylation. Our results suggest that the loss of p53 promotes ductular differentiation of hepatocyte‐derived tumor cells through either transdifferentiation or Myc‐mediated dedifferentiation.
Randomized Clinical Trial comparing clinical adjustment times of CAD/CAM screw‐retained posterior crowns on ti‐base abutments created with digital or conventional impressions. One‐year follow‐up
Authors: Derksen Wiebe , Tahmaseb Ali , Wismeijer Daniel 
Published: June 2021

AbstractObjectivesThe purpose of this randomized clinical trial was to compare the required time of potential clinical adjustments of posterior screw‐retained monolithic zirconia implant retained crowns based on intraoral optical scanning (IOS) or conventional impressions.Materials and methodsPatients with posterior tissue level implants (Straumann RN) replacing solitary teeth were recruited. Of all patients, impressions were taken with both an IOS (3M™ TDS) and a conventional (polyether) pick‐up impression. Randomization was performed after impression taking and patients were to receive either a crown based on the digital or the conventional impression. The time required for adjustments at placement was recorded. Additionally, restoration survival and mechanical complications with a follow‐up of one year were documented.ResultsThirty two patients with 45 implants were included: 23 restorations in the test (IOS) and 22 in the control (conventional) group. The average adjustment time was 3.35 min (SD ± 3.38, range: 0–11 min) for the digital versus 6.09 min (SD ± 4.63, range: 0–18 min) for the conventional impressions (p = .039). A proper fit (no adjustments required) was achieved 39,1% in the digital and 18,2% conventional group respectively. All 45 restorations could be placed within the two planned appointments and only two minor mechanical complications occurred during the first year of function.ConclusionsThe use of IOS resulted in shorter adjustment times at try‐in than conventional impressions for solitary CAD/CAM implant restorations. Screw‐retained solitary monolithic zirconia restorations on ti‐base abutments show low complication‐ and survival rates in the short term.
Assessment of the cytotoxic effects and chemical composition of the insoluble precipitate formed from sodium hypochlorite and chlorhexidine gluconate
Authors: Jeong Ji Wook , Sarmast Nima D. , Terlier Tanguy , van der Hoeven Ransome , Holland Julian N. , Parikh Neha 
Published: June 2021

AbstractAimTo investigate (1) the cytotoxic potential of the brown precipitate (BP) formed with sodium hypochlorite (NaOCl) and chlorhexidine gluconate (CHX), using both a small animal model of Caenorhabditis elegans (C. elegans) and cultured human gingival fibroblasts; and (2) the chemical composition of BP using Time‐of‐Flight Secondary Ion Mass Spectrometry (ToF‐SIMS).MethodologyBrown precipitate was obtained by mixing equal volumes of 6% NaOCl and 2% CHX and separating the BP from clear supernatant by centrifugation. The brown precipitate was weighed and solubilized in dimethyl sulfoxide for cytotoxicity experiments. The cytotoxic effect of BP was assessed using C. elegans larvae and primary immortalized human gingival fibroblasts‐hTERT (hTERT‐hNOF) cells. Various dilutions of BP (25 ng/µL–150 ng/µL), supernatant (0.15% v/v), NaOCl (1:100–1:1000 dilutions of 6% NaOCl) or CHX (1:500–1:1000 dilutions of 2% CHX) along with vehicle control (0.5% v/v ethanol and 0.15% v/v DMSO) or untreated control (growth medium) were tested on C. elegans larvae and hTERT‐hNOF cells. Viability was assessed in C. elegans larvae using stereomicroscopy and in hTERT‐hNOF cells using dehydrogenase‐based colorimetric assay. ToF‐SIMS was used to assess the chemical composition of BP in comparison with CHX and para‐chloroaniline (PCA). The C. elegans and cell line data were analysed using Log‐Rank test and Student's t‐test, respectively (p < .05).ResultsBP‐75 ng/µL and BP‐150 ng/µL were significantly more toxic to C. elegans larvae than the untreated, vehicle, supernatant or CHX treatment groups (p < .0001). Similarly, in hTERT‐hNOF cells, BP‐50 ng/µL, BP‐75 ng/µL and BP‐150 ng/µL induced significant cytotoxicity within 2 h compared with untreated, vehicle, supernatant and CHX treatments (p < .05). ToF‐SIMS analysis of BP revealed ion composition characteristic of both CHX and the carcinogen PCA.ConclusionsBrown precipitate was toxic in both C. elegans larvae and hTERT‐hNOF cells. The ToF‐SIMS analysis of BP revealed ions characteristic of CHX and PCA that could account for the toxicities observed in C. elegans larvae and human gingival fibroblasts. Because of the insoluble and toxic nature of BP, consecutive use of CHX and NaOCl irrigants should be avoided in root canal treatment.
A multicentric survey to evaluate preclinical education in Endodontology in German‐speaking countries
Authors: Sacha Sabine Rosa , Sonntag David , Burmeister Ulrike , Rüttermann Stefan , Gerhardt‐Szép Susanne 
Published: June 2021

AbstractAimTo evaluate preclinical education in Endodontology at Austrian, German and Swiss dental schools using an online survey.MethodologyAn online survey divided into nine categories was sent using SurveyMonkey software to 37 dental schools, before the spread of the COVID‐19 pandemic. The questionnaire included 50 questions to evaluate preclinical endodontic education, such as faculty‐to‐student ratios, topics taught and materials used, in preclinical phantom head courses. Seven and 14 days after the first e‐mail contact, dental schools received a reminder e‐mail. After four and six weeks, the dental schools were contacted by telephone and asked to participate in the online survey. The processing time was eight weeks in total.ResultsThe response rate was 89%. Preclinical endodontic education at the participating dental schools differs considerably. Theory classes ranged from 1 to 70 h (15 h mean), and practical classes ranged from 3 to 78 h (39 h mean). The faculty‐to‐student ratio varied between 1:4 and 1:38 (1:15 mean). Forty‐five per cent of the dental schools had a specialist in endodontics teaching theory. Several dental microscopes were available for preclinical teaching purposes at 82% of the dental schools. The majority (82%) taught root canal preparation with rotary or reciprocating NiTi instruments. Overall, 85% of the dental schools taught lateral compaction, amongst other methods, for canal filling.ConclusionA substantial divergence amongst the dental schools regarding the time dedicated to theory and practical instruction in Endodontology was reported. However, convergence in the use of root canal treatment techniques and materials was reported.
THE ECONOMICS OF SAVINGS GROUPS
Authors: Burlando Alfredo , Canidio Andrea , Selby Rebekah 
Published: June 2021

AbstractMillions of households worldwide rely on savings groups (SGs) to satisfy their financial needs, yet important gaps remain in our understanding of this novel financial institution. We show theoretically that, within an SG, the supply of funds could fall short or be in excess of its demand. Then, we use week‐by‐week records from 46 Ugandan SGs to show that most groups do not generate sufficient loanable funds. We conclude by proposing three interventions that, in light of our model, should ease credit rationing and improve the welfare of SG members: requiring SG members to publicly state their savings and borrowing goals, encouraging early savings, and linking SGs with formal financial institutions.
Cosmopolitanism and expatriate’s preference for host country food: The conditional effects of experiential capital and retail development
Authors: Miocevic Dario , Kvasina Antonija , Crnjak‐Karanovic Biljana 
Published: June 2021

AbstractIn this paper we explore the insufficiently addressed domain of expatriates’ preference for local (host country) food. Extant literature on this segment of consumers shows that tensions created by moving abroad are also reflected in their varying preference for host country foods. By drawing on social identity theory, we investigate how a cosmopolitan identity drives a preference for host country food under the contingent effects of an expatriate's experiential capital and host country's retail development. Based on a unique dataset of 293 expatriates living in six countries of the Greater Middle East, the findings from binary logistic regression model show that cosmopolitanism significantly explains expatriate's preference for host country food. We also show that the dimensions of experiential capital have countervailing moderating effects on this relationship whereas in countries with lower levels of retail development, the relationship between cosmopolitanism and expatriates’ preference for host country food becomes stronger. The findings of this study imply that cosmopolitan expatriates should not be considered a uniform segment as previous studies have advocated. Eventually, this study offers valuable contributions to literature that investigates the influence of socio‐cultural identities in food consumption as well as to literature dealing with expatriate consumer behaviour.
Relation of cardiac function to insulin resistance as evaluated by hyperinsulinemic‐euglycemic clamp analysis in individuals with type 2 diabetes
Authors: Otowa‐Suematsu Natsu , Sakaguchi Kazuhiko , Kaneko Akihiro , Ito Jun , Morita Yasuko , Miura Hiroshi , Yamada Tomoko , So Anna , Komada Hisako , Okada Yuko , Hirota Yushi , Tamori Yoshikazu , Ogawa Wataru 
Published: June 2021

AbstractAimsWhereas homeostasis model assessment of insulin resistance (HOMA‐IR), an easily measured but limited index of insulin resistance, has been shown to correlate with impairment of cardiac function in individuals without diabetes, the pathological relevance of insulin resistance to the development of cardiac dysfunction in individuals with type 2 diabetes has remained unclear. Here we investigated the relation between left ventricular (LV) function as assessed by echocardiography and insulin resistance as evaluated by hyperinsulinemic‐euglycemic clamp analysis, the gold standard for measurement of this parameter, in individuals with type 2 diabetes.MethodsThis retrospective study included 34 individuals with type 2 diabetes who underwent both hyperinsulinemic‐euglycemic clamp analysis and echocardiography. Both the insulin sensitivity index (ISI) as determined by glucose clamp analysis as well as HOMA‐IR were determined as measures of insulin resistance. The ratio of the peak early‐ to late‐diastolic mitral inflow velocities (E/A) and the LV ejection fraction (LVEF) were determined as measures of diastolic and systolic function, respectively.ResultsThe ISI was significantly correlated with both the E/A ratio and LVEF (correlation coefficients of 0.480 and 0.360, respectively), whereas HOMA‐IR was not correlated with either cardiac parameter. Multivariate analysis revealed that ISI was an independent predictor for both a high log [E/A] (P = 0.031) and a high LVEF (P = 0.045).ConclusionsInsulin resistance as evaluated by hyperinsulinemic‐euglycemic clamp analysis may be causally related to LV diastolic and systolic dysfunction in individuals with type 2 diabetes.
Gut microbiome and amyotrophic lateral sclerosis: A systematic review of current evidence
Authors: Sun Jiangwei , Huang Tingting , Debelius Justine W. , Fang Fang 
Published: June 2021

AbstractAmyotrophic lateral sclerosis (ALS), characterized by a loss of motor neurons in the brain and spinal cord, is a relatively rare but currently incurable neurodegenerative disease. The global incidence of ALS is estimated as 1.75 per 100,000 person‐years and the global prevalence is estimated as 4.1–8.4 per 100,000 individuals. Contributions from outside the central nervous system to the etiology of ALS have been increasingly recognized. Gut microbiome is one of the most quickly growing fields of research for ALS. In this article, we performed a comprehensive review of the results from existing animal and human studies, to provide an up‐to‐date summary of the current research on gut microbiome and ALS. In brief, we found relatively consistent results from animal studies, suggesting an altered gut microbiome composition in experimental ALS. Publication bias might however be a concern. Findings from human studies are largely inconclusive. A few animal and human studies demonstrated the usefulness of intervention with microbial‐derived metabolites in modulating the disease progression of ALS. We discussed potential methodological concerns in these studies, including study design, statistical power, handling process of biospecimens and sequencing data, as well as statistical methods and interpretation of results. Finally, we made a few proposals for continued microbiome research in ALS, with the aim to provide valid, reproducible, and translatable findings.