bluuebunny/crossref_metadata_2025_split huggingface dataset:
Voluntary Adolescent-Onset Alcohol Drinking Fails to Influence Alcohol Consumption or Anxiety-Like Behaviour in Adulthood in Female Alcohol-Preferring Rats
Authors: Mugantseva Ekaterina , Hyytiä Petri , Latvala Antti 
Published: August 2021

Abstract

Aims
Alcohol exposure during adolescence is associated with both increased risk for alcohol use disorders and anxiety in adulthood. Our present experiments examined this association using alcohol-preferring AA (Alko Alcohol) rats selected for high voluntary alcohol drinking.

Methods
Two groups of female AA rats acquired alcohol drinking at different ages. We gave the adolescent-onset group free choice to 10% alcohol and water for seven weeks, starting on post-natal day 42 (PND 42), whereas the adult-onset group started drinking alcohol on PND 112. After the 7-week drinking, we withdrew the adolescent group from alcohol for two weeks, followed by another voluntary 7-week drinking period, started at the same age as the adult-onset group. We assessed anxiety-like behaviour repeatedly during alcohol drinking with open field and elevated plus maze tests. At the end of alcohol drinking, we also tested the rats using the light/dark box, stress-induced body temperature test and social dominance test.

Results
During the first 7-week alcohol drinking, adolescent rats exhibited significantly slower acquisition of alcohol drinking and lower alcohol preference than the adult-onset group. However, when tested at the same age as the adult-onset rats, they displayed identical alcohol intake and preference. We found no alcohol-induced effects on anxiety- or stress-related behaviour in the experimental groups at any time points.

Conclusions
These data show that the genetically determined phenotype of high alcohol drinking of the female alcohol-preferring AA rats is not associated with a predisposition to develop anxiety-like behaviour following voluntary alcohol exposure, even when initiated during adolescence.
Distinctive Connectivities of Near-Stream and Watershed-Wide Land Uses Differentially Degrade Rural Aquatic Ecosystems
Authors: Jackson C Rhett , Cecala Kristen K , Wenger Seth J , Kirsch Joseph E , Webster Jackson R , Leigh David S , Sanders Jennifer M , Love Jason P , Knoepp Jennifer D , Fraterrigo Jennifer M , Rosemond Amy D 
Published: August 2021

AbstractThe water-quality effects of low-density rural land-use activities are understudied but important because of large rural land coverage. We review and synthesize spatially extensive studies of oligotrophic mountain streams in the rural Southern Appalachian Mountains, concluding that rural land-use activities significantly degrade water quality through altered and mostly enhanced landscape–stream connections, despite high forest retention. Some connections (insolation, organic inputs, root–channel interactions, stream–field connectivity, individual landowner discharges) are controlled by near-stream land-use activities, whereas others (reduced nitrogen uptake and cycling, enhanced biological nitrogen fixation, nutrient subsidy, runoff from compacted soils, road runoff delivery) are controlled by basin-wide land use. These connections merge to alter basal resources and shift fish, salamander, and invertebrate assemblages toward species tolerant of higher turbidity and summer temperatures and those more competitive in mesotrophic systems. Rural water quality problems could be mitigated substantially with well-known best management practices, raising socioecological governance questions about best management practice adoption.
Functional Differentiation of Dorsal and Ventral Posterior Parietal Cortex of the Rat: Implications for Controlled and Stimulus-Driven Attention
Authors: Yang Fang-Chi , Dokovna Lisa B , Burwell Rebecca D 
Published: August 2021

Abstract
The posterior parietal cortex (PPC) is important for visuospatial attention. The primate PPC shows functional differentiation such that dorsal areas are implicated in top–down, controlled attention, and ventral areas are implicated in bottom–up, stimulus-driven attention. Whether the rat PPC also shows such functional differentiation is unknown. Here, we address this open question using functional neuroanatomy and in vivo electrophysiology. Using conventional tract-tracing methods, we examined connectivity with other structures implicated in visuospatial attention including the lateral posterior nucleus of the thalamus (LPn) and the postrhinal cortex (POR). We showed that the LPn projects to the entire PPC, preferentially targeting more ventral areas. All parts of the PPC and POR are reciprocally connected with the strongest connections evident between ventral PPC and caudal POR. Next, we simultaneously recorded neuronal activity in dorsal and ventral PPC as rats performed a visuospatial attention (VSA ) task that engages in both bottom–up and top–down attention. Previously, we provided evidence that the dorsal PPC is engaged in multiple cognitive process including controlled attention (Yang et al. 2017). Here, we further showed that ventral PPC cells respond to stimulus onset more rapidly than dorsal PPC cells, providing evidence for a role in stimulus-driven, bottom–up attention.
Impaired Filtering and Hyperfocusing: Neural Evidence for Distinct Selective Attention Abnormalities in People with Schizophrenia
Authors: Hahn Britta , Robinson Benjamin M , Kiat John E , Geng Joy , Bansal Sonia , Luck Steven J , Gold James M 
Published: August 2021

Abstract
Although schizophrenia is classically thought to involve impaired attentional filtering, people with schizophrenia (PSZ) exhibit a more intense and more exclusive attentional focus than healthy control subjects (HCS) in many tasks. To resolve this contradiction, this functional magnetic resonance imaging study tested the impact of attentional control demands on the modulation of stimulus-induced activation in the fusiform face area and parahippocampal place area when participants (43 PSZ and 43 HCS) were looking for a target face versus house. Stimuli were presented individually, or as face-house overlays that challenged attentional control. Responses were slower for house than face stimuli and when prioritizing houses over faces in overlays, suggesting a difference in salience. Blood-oxygen-level-dependent activity reflected poorer attentional selectivity in PSZ than HCS when attentional control was challenged most, that is, when stimuli were overlaid and the task required detecting the lower-salience house target. By contrast, attentional selectivity was exaggerated in PSZ when control was challenged least, that is, when stimuli were presented sequentially and the task required detecting the higher-salience face target. These findings are consistent with 2 distinct attentional abnormalities in schizophrenia leading to impaired and exaggerated selection under different conditions: attentional control deficits, and hyperfocusing once attention has been directed toward a stimulus.
Early Start of Infliximab in Crohn’s Disease Increases Rates of Endoscopic Remission and Decreases Stenosis Formation: Experiences From a Single Center Cohort
Authors: Schnitzler Fabian , Seitz Theresia , Tillack-Schreiber Cornelia , Lange Silke , Waggershauser Constanze , Ochsenkühn Thomas 
Published: August 2021

Abstract

Background
Over 10 years ago, the step-up/top-down trial demonstrated favorable outcomes of Crohn’s disease (CD) after early initiation of infliximab (IFX) in patients with CD. However, data on long-term effects of this treatment strategy in daily clinical practice are scarce.

Methods
This retrospective study investigated effects of early (&lt;24 months after diagnosis) versus late intervention (&gt;24 months) of IFX in CD on endoscopic remission (ER) rates, surgery rates, and course of CD, long term.

Results
Overall, 242 CD patients (94 early, 148 late intervention) were started on IFX and followed for 24 months. Sixty-one patients with early and 86 with late intervention underwent endoscopy after start of IFX. After IFX induction, 90.3% of patients with early versus 87.8% with late intervention were in clinical remission (P = .676), compared to 89.1% versus 85.8% after 24 months (P = .554). Almost half of patients with early IFX (45.9%, n = 28/61) achieved ER within 24 months compared to only one forth with late IFX intervention (25.6%, n = 22/86, P = .013). In addition, significantly less patients with early IFX intervention (9.8%, n = 6/61) developed intestinal stenosis during 24 months follow-up compared to late IFX start (29.1%, n = 25/86, P = .007). Logistic regression revealed early IFX intervention as only relevant factor achieving ER with an odds ratio of 2.386 (95% confidence interval [1.1180; 4.825], P = .016).

Conclusions
Our data on early IFX therapy in CD support early IFX intervention with more patients achieving ER, and less patients developing stricturing disease behavior. Early IFX intervention could therefore change the course of CD.
Efficacy and Safety of Subcutaneous Vedolizumab in Patients With Moderately to Severely Active Crohn’s Disease: Results From the VISIBLE 2 Randomised Trial
Authors: Vermeire Séverine , D’Haens Geert , Baert Filip , Danese Silvio , Kobayashi Taku , Loftus Edward V , Bhatia Siddharth , Agboton Christian , Rosario Maria , Chen Chunlin , Zhang Wenwen , Kisfalvi Krisztina , Sandborn William J 
Published: August 2021

Abstract

Background and Aims
To report results from VISIBLE 2, a randomised, double-blind, placebo-controlled, phase 3 trial evaluating a new subcutaneous [SC] vedolizumab formulation as maintenance treatment in adults with moderately to severely active Crohn’s disease [CD].

Methods
Following open-label vedolizumab 300 mg intravenous induction therapy at Weeks 0 and 2, Week 6 clinical responders (≥70-point decrease in CD Activity Index [CDAI] score from baseline) were randomised 2:1 to receive double-blind maintenance vedolizumab 108 mg SC or placebo every 2 weeks until Week 50. Assessments at Week 52 included clinical remission [primary endpoint; CDAI ≤150], enhanced clinical response [≥100-point decrease in CDAI from baseline], corticosteroid-free clinical remission among patients using a corticosteroid at baseline, clinical remission in anti-tumour necrosis factor [anti-TNF]-naïve patients, and safety.

Results
Following vedolizumab intravenous induction, 275 patients were randomised to vedolizumab SC and 135 to placebo maintenance. At Week 52, 48.0% of patients receiving vedolizumab SC versus 34.3% receiving placebo were in clinical remission [p = 0.008]. Enhanced clinical response at Week 52 was achieved by 52.0% versus 44.8% of patients receiving vedolizumab SC versus placebo, respectively [p = 0.167]. At Week 52, 45.3% and 18.2% of patients receiving vedolizumab SC and placebo, respectively, were in corticosteroid-free clinical remission, and 48.6% of anti-TNF-naïve patients receiving vedolizumab SC and 42.9% receiving placebo were in clinical remission. Injection site reaction was the only new safety finding observed for vedolizumab SC [2.9%].

Conclusions
Vedolizumab SC is an effective and safe maintenance therapy in patients with CD who responded to two infusions of vedolizumab intravenous induction therapy.
Microbially mediated nitrogen removal and retention in the York River Estuary
Authors: G. Fortin Samantha , Song Bongkeun , C. Anderson Iris 
Published: August 2021

ABSTRACT
Denitrification, anaerobic ammonium oxidation and dissimilatory nitrate reduction to ammonium (DNRA) are important microbial processes determining the fate of nitrogen (N) in estuaries. This study examined these processes in sediments of the York River Estuary, a tributary of Chesapeake Bay, and investigated environmental and microbial drivers of the rates of denitrification and DNRA. Nitrate reduction followed a consistent pattern throughout the year and across the estuary with nitrogen removal, primarily through denitrification, decreasing from the head of the estuary to the mouth and nitrogen retention, through DNRA, following the opposite pattern. At the mouth of the estuary, nitrogen retention was consistently higher than nitrogen removal. Denitrification rates showed strong linear relationships with concentrations of organic matter, nitrate and chlorophyll a, and the abundance of the nirS gene. DNRA rates were best correlated with the relative abundance of three bacterial families, Anaerolineaceae,Ectothiorhodospiraceae and Prolixibacteraceae, which carry the nrfA gene. The controls responsible for retention or removal of N from an estuary are complex, involving both geochemical and microbial factors. The N retained within estuaries may support primary production and seasonal algae blooms and result in estuarine eutrophication.
Efficacy of Clonal Propagation in Andaman Padauk (Pterocarpus dalbergioides): Augmenting Conservation of an Endemic Ornamental Timber Species of Andaman & Nicobar Islands, India
Authors: Jaisankar Iyyappan , Varadan R Jayakumara , Vijayakumar S , Jambhulkar Nitiprasad Namdeorao , Jerard Bosco Augustine , Ganguly Nabanita , Rajkumar Muthu , Moses Ezekiel Manasseh , Simhachalam Putcha 
Published: August 2021

Abstract
The population of Andaman padauk (Pterocarpus dalbergioides Roxb.), an endemic ornamental timber species, is fast depleting in the Andaman Islands. Afforestation through seedlings is hindered by low germination and high mortality rates. This study attempts to assess the efficacy of clonal propagation methods to hasten mass multiplication by trying out different combinations of growth hormones, concentration, and dipping duration in semi-hardwood stem cuttings and different combinations of growth hormone concentration and substrate in air layering. Dipping the cuttings in indole-3-butyric acid (IBA) 1,500 ppm for 24 hours and spraying the modified stem-cut layers with IBA 2,000 ppm and binding with top soil and decomposed coir dust turned out to be the best treatment combination for cutting and air layering, respectively. Among the considered factors, the concentration of growth hormone is crucial for cutting, as the performance is enhanced with concentration under a given growth hormone and dipping duration. Similarly, the substrate is crucial for air layering, as modified stem-cut air layers bound with top soil and decomposed coir dust not only achieve the best performance, but also catalyze IBA for further improvement when other substrates remain unresponsive. Successful outcome of both clonal propagation techniques gives hope for disseminating the species across the tropics at global level.
Volatility Decay and Diversification Role of Timberland as the Investment Horizon Lengthens
Authors: Zhang Weiyi , Mei Bin 
Published: August 2021

Abstract
Under the mean-variance efficiency framework, we investigate the role of timberland asset in a mixed-asset portfolio in the United States. Starting from a single period (quarterly) view, we first reveal the crucial role of serial correlation in defining an asset’s financial performance. Accordingly, we modify return, volatility, and correlation for multiyear horizons to account for the nature of long-term investments. Our results show that, although timberland is persistent in all portfolios, private-equity timberland can be substituted by other liquid assets, including public-equity timberland, as the lengthening holding period substantially reduces their volatilities. We conclude that private-equity timberland is a risk diversifier regardless of the length of the investment horizon, whereas public-equity timberland becomes a suitable diversifier only for long-term investors with high risk tolerance.
Speeding up eQTL scans in the BXD population using GPUs
Authors: Trotter Chelsea , Kim Hyeonju , Farage Gregory , Prins Pjotr , Williams Robert W , Broman Karl W , Sen Śaunak 
Published: August 2021

Abstract
The BXD family of mouse strains are an important reference population for systems biology and genetics that have been fully sequenced and deeply phenotyped. To facilitate interactive use of genotype–phenotype relations using many massive omics data sets for this and other segregating populations, we have developed new algorithms and code that enable near-real-time whole-genome quantitative trait locus (QTL) scans for up to one million traits. By using easily parallelizable operations including matrix multiplication, vectorized operations, and element-wise operations, our method is more than 700 times faster than a R/qtl linear model genome scan using 16 threads. We used parallelization of different CPU threads as well as GPUs. We found that the speed advantage of GPUs is dependent on problem size and shape (the number of cases, number of genotypes, and number of traits). Our approach is ideal for interactive web services, such as GeneNetwork.org that need to display results in real-time. Our implementation is available as the Julia language package LiteQTL at https://github.com/senresearch/LiteQTL.jl.
BPIFB4 Circulating Levels and Its Prognostic Relevance in COVID-19
Authors: Ciaglia Elena , Lopardo Valentina , Montella Francesco , Sellitto Carmine , Manzo Valentina , De Bellis Emanuela , Iannaccone Teresa , Franci Gianluigi , Zannella Carla , Pagliano Pasquale , Di Pietro Paola , Carrizzo Albino , Vecchione Carmine , Conti Valeria , Filippelli Amelia , Puca Annibale Alessandro 
Published: August 2021

Abstract
Aging and comorbidities make individuals at greatest risk of COVID-19 serious illness and mortality due to senescence-related events and deleterious inflammation. Long-living individuals (LLIs) are less susceptible to inflammation and develop more resiliency to COVID-19. As demonstrated, LLIs are characterized by high circulating levels of BPIFB4, a protein involved in homeostatic response to inflammatory stimuli. Also, LLIs show enrichment of homozygous genotype for the minor alleles of a 4 missense single-nucleotide polymorphism haplotype (longevity-associated variant [LAV]) in BPIFB4, able to counteract progression of diseases in animal models. Thus, the present study was designed to assess the presence and significance of BPIFB4 level in COVID-19 patients and the potential therapeutic use of LAV-BPIFB4 in fighting COVID-19. BPIFB4 plasma concentration was found significantly higher in LLIs compared to old healthy controls while it significantly decreased in 64 COVID-19 patients. Further, the drop in BPIFB4 values correlated with disease severity. Accordingly to the LAV-BPIFB4 immunomodulatory role, while lysates of SARS-CoV-2-infected cells induced an inflammatory response in healthy peripheral blood mononuclear cells in vitro, the co-treatment with recombinant protein (rh) LAV-BPIFB4 resulted in a protective and self-limiting reaction, culminating in the downregulation of CD69 activating-marker for T cells (both TCD4+ and TCD8+) and in MCP-1 reduction. On the contrary, rhLAV-BPIFB4 induced a rapid increase in IL-18 and IL-1b levels, shown largely protective during the early stages of the virus infection. This evidence, along with the ability of rhLAV-BPIFB4 to counteract the cytotoxicity induced by SARS-CoV-2 lysate in selected target cell lines, corroborates BPIFB4 prognostic value and open new therapeutic possibilities in more vulnerable people.
Application of explicit precautionary principles in data-limited fisheries management
Authors: Fischer Simon H , De Oliveira José A A , Mumford John D , Kell Laurence T 
Published: August 2021

Abstract
Many management bodies require applying the precautionary approach when managing marine fisheries resources to achieve sustainability and avoid exceeding limits. For data-limited stocks, however, defining and achieving management objectives can be difficult. Management procedures can be optimized towards specific management objectives with genetic algorithms. We explored the feasibility of including an objective that limited the risk of a stock falling below various limit reference points in the optimization routine for an empirical data-limited control rule that uses a biomass index, mean catch length, and includes constraints (the “rfb-rule”). This was tested through management strategy evaluation on several fish stocks representing various life-history traits. We show that risk objectives could be met, but more restrictive risk limits can lead to a potential loss of yield. Outcomes were sensitive to simulation conditions such as observation uncertainty, which can be highly uncertain in data-limited situations. The rfb-rule outperforms the method currently applied by ICES, particularly when risk limitation objectives are considered. We conclude that the application of explicit precautionary levels is useful to avoid overfishing. However, we caution against the indiscriminate use of arbitrary risk limits without scientific evaluation to analyse their impact on stock yields and sustainability.
Advantages and Challenges of Using Breast Biopsy Markers
Authors: Alshafeiy Taghreed I , Matich Alison , Rochman Carrie M , Harvey Jennifer A 
Published: August 2021

AbstractPercutaneous image-guided biopsy procedures are the standard of care for histologic assessment of suspicious breast lesions. Post-biopsy tissue markers (clips) optimize patient management by allowing for assessment on follow-up imaging and precise lesion localization. Markers are used to ensure accurate correlation between imaging modalities, guide preoperative localization for malignant and high-risk lesions, and facilitate accurate identification of benign lesions at follow-up. Local practices differ widely, and there are no data detailing the exact frequency of use of clips for different breast biopsies. There are many indications for biopsy marker deployment, and some difficulties may be encountered after placement. The placement of biopsy markers has many advantages and few disadvantages, such that deployment should be routinely used after percutaneous biopsy procedures with rare exception.
A new species ofSalentinellaRuffo, 1947 from a thermo-mineral cave in southern Spain, with comments on the systematic position of the family Salentinellidae (Amphipoda)
Authors: Jaume Damià , Vonk Ronald 
Published: August 2021

AbstractA new species of the stygobiont amphipod genus SalentinellaRuffo, 1947 is described from a thermo-mineral cave of Granada, southern Spain. Detailed observation of the appendages of the new species and of fresh specimens of S. angelieriDelamare-Deboutteville & Ruffo, 1952, and their comparison with the descriptions of other members of the family, have revealed that a simple callynophore is present on the antennule in both sexes of salentinellids. Furthermore, salentinellids have resulted to show a non-senticaudatan (i.e., lack of apical spines on rami) condition of uropods I and II. Both features question the current placement of the family within the senticaudatan parvorder Bogidiellidira. An identification key to all known members of the family Salentinellidae is provided.
The Uses of Stages of Change and Language Within a Forestry Context
Authors: Sarmiento Irene G , Van Swol Lyn M , Shaw Bret R , Chung Jennifer H , Koshollek Alanna 
Published: August 2021

AbstractAdvising landowners can be difficult for foresters. Landowners’ motivations towards behavior change should be considered prior to giving advice. The Stages of Change Model, also known as the Transtheoretical Model, assesses whether individuals are ready to perform new behaviors. This model was used to understand how landowner’s motivations towards behavior change affect future reforestation efforts, which could help foresters better advise landowners. Landowners from Wisconsin were recruited, reported current behaviors, and separated into either predecisional or postdecisional stages, based on self-reported stage of behavior. They received advice, reported future reforestation intentions, and provided comments. Using Ordinary Least Squares (OLS) regression and language analyses, predecisional stage perceived more barriers towards reforestation efforts and used more negative emotion words, whereas postdecisional stage reported more past habits of reforestation efforts and used more positive emotion words. Foresters should provide advice that reduces barriers for the predecisional stage and build on positive aspects of reforestation for the postdecisional stage.