bluuebunny/crossref_metadata_2025_split huggingface dataset:
Transcriptome analysis and reverse engineering verification of SNZ3 Val125Ile and Pho3 Asn134Asp revealed the mechanism of laboratory adaptive evolution to increase the yield of tyrosol in Saccharomyces cerevisiae S26
Authors: Song Na , Xia Huili , Yang Xiaoxue , Liu Siyao , Xu Linglong , Zhuang Kun , Yao Lan , Yang Shihui , Chen Xiong , Dai Jun 
Published: December 2024

Abstract

Background
Tyrosol is an important drug precursor, and Saccharomyces cerevisiae is one of the main microorganisms producing tyrosol. Although excessive metabolic modification increased the production of tyrosol, it also caused a decrease in the growth rate of yeast. Therefore, this study attempted to restore the growth of S. cerevisiae through adaptive evolution and further improve tyrosol production.
Results
After the adaptive laboratory evolution of S. cerevisiae S26, three evolutionary strains were obtained. The biomass of strain S26-AE2 reached 17.82 under the condition of 100 g/L glucose which was 15.33% higher than that of S26, and its tyrosol production reached 817.83 mg/L. Transcriptome analysis showed that the strain S26-AE2 may through decreased expression of HXK2 reduce the transcriptional regulation of glucose repression and increase the expression of gene PGI1 to promote the utilization of glucose. The genes related to pyruvate synthesis were enhanced in strain S26-AE2. Under the 20 g/L glucose condition, the TCA cycle-related genes of the S26-AE2 were more active. Furthermore, the tyrosol production of S26 with SNZ3Val125Ile mutation increased by 17.01% compared with the control strain S26 under the condition of 100 g/L glucose.
Conclusions
In this paper, a strain S26-AE2 with good growth and tyrosol production performance was obtained by adaptive evolution. The transcriptome reveals the differences in gene expression in metabolic pathways of adaptive evolutionary strains may be related to the growth of yeast and the production of tyrosol. Further reverse engineering verified the mutation of SNZ3 promoted the synthesis of tyrosol in S. cerevisiae in the glucose-rich medium. This study provides a theoretical basis for the metabolic engineering of S. cerevisiae to synthesize tyrosol and its derivatives.
Comparative study of heat input in conventional and advanced short circuit GMAW variants
Authors: Wiggers João Francisco , Silva Régis Henrique Gonçalves 
Published: December 2024

Abstract

Due to its varied range of applications, the short circuit GMAW process is highly used in the industry. Among the welding processes available in the market, this one stands out for delivering low cost and high productivity, being commonly used in naval field as root pass and also in additive manufacture for turbines production. Nevertheless, the heat input applied for this process is one of the main challenges when regarding applications where sensitive materials are used and precision is required, since high temperatures imply residual stress and possible structural damages. Therefore, the short circuiting metal transfer technologies developed by different companies are tested to study their methods to control the temperature in the welding process. This knowledge may help to understand the influences of the parameters and, then, to parameterize the process and select a technology among so many available in the market. The processes divided in standard, electrically controlled and retractable wire feed short circuit are used to produce a weld bead with similar geometry and have their performance analyzed developing the same task. As one of the most recent applications of modified short circuit GMAW, an exploratory additive manufacturing test is developed with three different processes to compare their methods and performances. The electrical parameters of each process are measured and the theoretical heat input is calculated and compared. A macrograph of the result weld beads is made to analyze the penetration and its relation with the deposition. It was observed throughout the heat input and penetration areas comparison that the theoretical heat input does not describe the physical performance of the processes. Some samples showed higher theoretical heat input and smaller penetration than others. Therefore, the physical performance can be better predicted by observing the process electric waveform, where drop detachment frequency, power peak and low current intervals are very influential. The standard short circuit processes showed the highest penetrations, followed by the electrical controlled processes. The retractable wire feed processes showed the lowest penetration values. In the theoretical heat input measured was not possible to observe significant advantages between the electrical controlled and standard processes, with exception for the ECP 2 technology. The retractable wire feed processes had the best results in the metrics, showing the lowest theoretical heat input and penetration. The preliminary AM procedure showed a big range of geometry changes, with the retractable wire feed processes controlling better the heat input, leading to a more continuous part.
Experimental Study on Abrasive Flow Polishing of Grooves and Oil Holes of Aircraft Engine Main Bearing Ring
Authors: An.Shi.Chen  , wumingyu  , Yi.Lan.Long  , Xv.Wu  , A.Qiang.Xv  , Qing.Hao.Zhang  
Published: December 2024

Abstract

This study addresses the challenges in machining the raceways and oil holes of aircraft engine bearing rings by conducting abrasive flow machining experiments on main bearing rings which had undergone ultra-precision grinding. Viscoelastic abrasive media containing cubic boron nitride of different particle sizes is used during the experiments. The results show that bearing performance is improved significantly in terms of surface roughness, surface microtopography, residual compressive stress, and oil film thickness, consequently, the overall surface quality is raised. The machining process meets the precision requirements for the main bearings of this type of aircraft engine, validating the feasibility and effectiveness of Abrasive Flow Machining( AFM). And the foundation for further optimization of this process is set through this research.
Unlocking single-cell level and whole-slide insights in spatial transcriptomics with PanoSpace
Authors: He Hui-Feng , Peng Pai , Yang Shi-Tong , Wang Meng-Guo , Chen Luonan , Zhang Xiao-Fei 
Published: December 2024

Abstract

Spatial transcriptomics has significantly advanced our ability to map gene expression within native tissue contexts. However, current low-resolution technologies are constrained by limited spatial resolution and tissue coverage. We present PanoSpace, a novel computational framework that integrates low-resolution spatial transcriptomics data with high-resolution histological images and matched single-cell RNA sequencing references. PanoSpace achieves comprehensive single-cell level and whole-tissue analysis by accurately inferring spatial localization, cell type, and gene expression for all cells across entire tissue slides. It also facilitates exploration of intra-cell-type heterogeneity and cell-cell interactions within spatial contexts. Application of PanoSpace to breast, prostate, and cervical cancer tissues reveals detailed cell-type distributions and gene expression patterns with unprecedented resolution and coverage. Furthermore, through analysis of interactions with cancer-associated fibroblasts, PanoSpace uncovers intra-cell-type heterogeneity and provides novel insights into tumor microenvironment dynamics. These findings highlight PanoSpace as a powerful tool for offering insights beyond the reach of existing technologies and computational methods.
Water impact analysis due to coal-electricity generation using the life cycle assessment method: a case study in Malaysia
Authors: Mardi Nurul Hani , Ean Lee Woen , Malek Marlinda Abdul , Chua Kok Hua , Ahmed Ali Najah 
Published: December 2024

ABSTRACT

Coal power plants adversely impact air pollution, but they also pose a risk to our water sources. Discharge wastewater from power plants may degrade the quality of nearby water bodies. This study evaluates the potential water-related environmental impacts of electricity generation at an ultra-supercritical coal power plant in Malaysia using the life cycle assessment method. The inventory data were gathered from a Malaysian power plant, and supporting data were taken from the relevant literature. Utilizing the ReCiPe 2016 impact assessment method, this study analyses the mid-point impact categories of freshwater eutrophication (FEP), marine eutrophication (MEP), freshwater ecotoxicity (FETP), and marine ecotoxicity (METP). The results indicate that METP is the leading risk, with an average impact of 1.94 × 10−2 kg 1,4-DCB per kWh electricity generated, followed by FETP (1.40 × 10−2 kg 1,4-DCB), FEP (4.66 × 10−4 kg P eq), and MEP (2.95 × 10−5 kg N eq). About 95% of the mid-point impact is due to the extraction and processing of hard coal. These findings underscore a critical aspect of environmental management at the supply chain level. Furthermore, mitigating direct emissions from power generation could reduce the mid-point impact, as demonstrated by comparisons with previous research.
Liquefaction Potential Analysis on Pandansimo Bridge using Nonlinear Site Response Analysis
Authors: Pramudyaningrum Astri Tamara , Rifa’i Ahmad , Fathani Teuku Faisal 
Published: December 2024

Background
The construction of bridges is one of the infrastructures that support the Trans South-South Java Road in Yogyakarta Province, including the Pandansimo Bridge. This bridge is located in a high liquefaction vulnerability zone near the coastal area with a shallow groundwater table, composed of saturated sandy soil and less than 10 km from the active Opak fault.

Objective
The study aims to evaluate the liquefaction potential in the Pandansimo Bridge area with the scenario of the 2006 Yogyakarta earthquake of 6.3 Mw.

Methods
The evaluation used eight modified pairs of ground motion to obtain peak ground acceleration values through nonlinear calculations with site-specific response analysis. Furthermore, the liquefaction potential analysis was conducted based on these results using simplified procedure methods.

Results
The results show that each soil layer’s peak ground acceleration value ranged from 0.30 g to 0.49 g. Liquefaction potential is at a depth of 1 m to 16 m, with various thicknesses for each borehole depending on the soil properties, with vulnerability levels varying from very low to very high.

Conclusion
The findings indicate that liquefaction potential occurs at several borehole locations at Pandansimo Bridge. This liquefaction condition may serve as a reference for mitigating strategies applicable in case of probable liquefaction in the infrastructure.
EARLY LIVE OVARIAN ECTOPIC GESTATION, CAN THE OVARY BE PRESERVED? : A CASE REPORT
Authors: Adiga Chaitra , Vinekar Sripada , Prasad Mohini N , Mahesh Deepa 
Published: December 2024

Ovarian ectopic gestation is often missed or misdiagnosed and occurs on or within the ovary. Owing to the similar appearances of ovarian pregnancy and corpus luteum cyst, the diagnosis is not straightforward when yolk sac and fetal pole are absent. We hereby report a rare case of early live ectopic gestation in the ovary at 6 weeks of amenorrhea. Ultrasound examination of the pelvis showed, no intrauterine gestation, but a corpus luteum cyst with associated hemoperitoneum and a thick-walled adnexal cyst close to the ovary with fetal node and cardiac activity within confirming ectopic pregnancy. She underwent emergency laparoscopic cyst removal, preserving the ovary and is doing well.
Keywords: ectopic pregnancy, laparoscopic cystectomy, live ectopic, ovarian ectopic
Integrated Use of Autosomal Dominant Polycystic Kidney Disease Prediction Tools for Risk Prognostication
Authors: Wolff Constantin A. , Aiello Valeria , Elhassan Elhussein A.E. , Cristalli Carlotta , Lerario Sarah , Paccapelo Alexandro , Ciurli Francesca , Montanari Francesca , Conti Amalia , Benson Katherine , Seri Marco , Brigl Carolin B. , Münster Julia S. , Sciascia Nicola , Kursch Sebastian , de Fallois Jonathan , La Manna Gaetano , Eckardt Kai-Uwe , Rank Nina , Popp Bernt , Schönauer Ria , Conlon Peter J. , Capelli Irene , Halbritter Jan 
Published: December 2024

Key Points

The Mayo clinic imaging classification and the predicting renal outcome in polycystic kidney disease score are used to assess the risk of progression to kidney failure in autosomal dominant polycystic kidney disease.Mayo imaging classification and predicting renal outcome in polycystic kidney disease show little concordance; combined use increased the ability to identify rapid progression especially among intermediate risk patients.Accurate risk prediction is key for determining indication for specific treatment.

Background
Autosomal dominant polycystic kidney disease is the most common genetic cause of kidney failure. Specific treatment is indicated on observed or predicted rapid progression. For the latter, risk stratification tools have been developed independently based on either total kidney volume or genotyping as well as clinical variables. This study aimed to improve risk prediction by combining both imaging and clinical-genetic scores.

Methods
We conducted a retrospective multicenter cohort study of 468 patients diagnosed with autosomal dominant polycystic kidney disease. Clinical, imaging, and genetic data were analyzed for risk prediction. We defined rapid disease progression as an eGFR slope ≥3 ml/min per 1.73 m2 per year over 2 years, Mayo imaging classification (MIC) 1D–1E, or a predicting renal outcome in polycystic kidney disease (PROPKD) score of ≥7 points. Using MIC, PROPKD, and rare exome variant ensemble learner scores, several combined models were designed to develop a new classification with improved risk stratification. Primary endpoints were the development of advanced CKD stages G4–G5, longitudinal changes in eGFR, and clinical variables such as hypertension or urological events. Statistically, logistic regression, survival, receiver operating characteristic analyses, linear mixed models, and Cox proportional hazards models were used.

Results

PKD1-genotype (P < 0.001), MIC class 1E (P < 0.001), early-onset hypertension (P < 0.001), and early-onset urological events (P = 0.003) correlated best with rapid progression in multivariable analysis. While the MIC showed satisfactory specificity (77%), the PROPKD was more sensitive (59%). Among individuals with an intermediate risk in one of the scores, integration of the other score (combined scoring) allowed for more accurate stratification.

Conclusions
The combined use of both risk scores was associated with higher ability to identify rapid progressors and resulted in a better stratification, notably among intermediate risk patients.
Kritikk av kritisk danning
Authors: Æsøy Knut Ove 
Published: December 2024

The chapter undertakes a theoretical investigation of critical formation. In the introduction, I explain the term critical formation, tracing its historical roots from the Enlightenment and examining its place in Norwegian educational thinking during the 1970s and beyond. The introduction culminates in showing the significance of critical formation in fostering an environment where individuals are encouraged to think independently.
The main section presents three criticisms of critical formation, emphasizing that it should function more as a watchdog than as an end in itself. The first critique questions the tendency of critical thinking to problematize, analyze, and complicate every aspect of existence. The second critique suggests that critical formation may result in self-criticism and an increased demand for conscious action. The third critique investigates how critical formation may limit the scope for a person’s existential, theological, and metaphysical dimensions.
In the final part of the chapter, I try to unfold a constructive ability to acknowledge reality independent of criticism, and call this ‘resonance’. The purpose is not to reject criticism, but to open a dialectical movement between, on the one hand, criticism and, on the other, resonance and open trust. Both resonance and trust, grounded in faith in the world, should serve as the basis and ultimate goal of our capacity to be in the world.
KORELASI ANTARA POLA ASUH DAN KEBIASAAN BELAJAR TERHADAP PRESTASI MATEMATIKA SISWA SMA DI MASA PANDEMI
Authors: Afini Arsyi 
Published: December 2024

Penelitian bertujuan untuk mengetahui korelasi antara pola asuh terhadap prestasi matematika, kebiasaan belajar terhadap prestasi matematika dan secara bersama-sama antara pola asuh dan kebiasaan belajar terhadap prestasi matematika. Jenis penelitian ini menggunakan pendekatan kuantitatif dengan metode Ex Post Facto. Sampel yang diteliti sebanyak 30 siswa kelas XI SMA. Pengumpulan data yang digunakan yaitu kuisioner skala likert dan nilai UAS. Teknik analisis data menggunakan uji prasyarat (deskriptif statistik, normalitas, linieritas dan multikolinieritas) dan uji korelasi (Korelasi Product Moment dan Multiple Correlation). Hasil penelitian diperoleh bahwa (1) tidak terdapat korelasi yang signifikan antara pola asuh terhadap prestasi matematika sebesar -0.234; (2) terdapat korelasi yang signifikan antara kebiasaan belajar terhadap prestasi matematika sebesar 0.398; (3) terdapat korelasi yang signifikan antara pola asuh dan kebiasaan belajar terhadap prestasi matematika sebesar 0.424 dengan tingkat kontribusi 18% dari variabel-variabel.
Kata kunci : Pola Asuh, Kebiasaan Belajar dan Prestasi Matematika
PENERAPAN MODEL PEMBELAJARAN KOOPERATIF TIPE TALKING STICK UNTUK MENINGKATKAN MINAT BELAJAR MATEMATIKA PESERTA DIDIK DI KELAS XI MIPA 3 SMA NEGERI 1 KUTA UTARA
Authors: Pande Putu Dyah Maharani  , Jayantika I Gusti Agung Ngurah Trisna 
Published: December 2024

ABSTRAK
Penelitian ini merupakan penelitian dengan jenis penelitian tindakan kelas (PTK) yang bertujuan untuk meningkatkan minat belajar matematika dengan menerapkan model pembelajaran kooperatif tipe talking stick. Desain penelitian ini terdiri dari 2 siklus dengan subjek penelitian adalah peserta didik di kelas XI MIPA 3 SMA Negeri 1 Kuta Utara sebanyak 45 peserta didik pada tahun ajaran 2022/2023. Tahapan penelitian pada setiap siklusnya terdiri dari perencanan (planning), pelaksanaan Tindakan (action), pengamatan tindakan (observation), dan refleksi (reflection). Instrumen penelitian yang digunakan yaitu angket untuk mengukur minat belajar matematika peserta didik. Pada pra siklus peserta didik memiliki rata-rata minat belajar yang dikategorikan sedang dengan persentase 66,2% dan terdapat 11,1% (5 peserta didik) dengan minat belajar tinggi. Pada siklus I, rata-rata minat belajar berada pada kategori sedang dengan persentase 73,2% dan persentase peserta didik yang memiliki minat belajar tinggi adalah 42,2% (19 peserta didik). Pada siklus II, persentase rata-rata minat belajar peserta didik berada pada kategori tinggi dengan persentase 79,5% dan terdapat 80% yang memiliki minat belajar tinggi (33 peserta didik dengan minat belajar tinggi dan 3 peserta didik dengan minat belajar sangat tinggi). Hal tersebut menunjukkan terjadinya peningkatan minat belajar peserta didik dengan menerapkan model pembelajaran kooperatif tipe talking stick.
Kata Kunci: Minat belajar, Talking Stick, Pembelajaran Kooperatif

Abstract
This research is Classroom Action Research (CAR) that aims to increase students’ interest in learning mathematics by applying a talking stick type cooperative learning model. The design of this research consists of 2 cycles. 45 students of class XI MIPA 3 SMA Negeri 1 Kuta Utara in the year of 2022/ 2023 were selected as research subjects. The research stages in each cycle consist of planning, action, observation, and reflection. The research instrument used was a questionnaire to measure students' interest in learning mathematics. In the pre-cycle, students have an average interest in learning which is categorized as moderate with a percentage of 66.2% and there are 11.1% (5 students) with a high interest in learning. In the cycle I, the average interest in learning was in the moderate category with a percentage of 73.2% and the percentage of students who had a high interest in learning was 42.2% (19 learners). In cycle II, the average percentage of students' interest in learning is in the high category with a percentage of 79.5% and there are 80% who have a high interest in learning (33 students with high interest in learning and 3 students with very high interest in learning). The findings showed an increase in students' interest in learning by applying a talking stick-type cooperative learning model.
Keyword: Learning interest, talking stick, cooperative learning
PEMBELAJARAN MATEMATIKA PRAKTIS DAN MENYENANGKAN BERBASIS SMART SOLUTION PADA MATERI VOLUME BANGUN RUANG DI SD/MI
Authors: Fachrudin  , Mohamad Shokeh  , M. Nur Ghufron  , Muhamad Jalil  
Published: December 2024

Pembelajaran matematika praktis dan menyenangkan sebagai bagian dari solusi agar pelajaran matematika bukan lagi dianggap pelajaran yang sulit dan menakutkan. Tujuan penelitian ini: (1)menganalisis inovasi proses pembelajaran matematika pasca pandemi praktis dan menyenangkan berbasis smart solution bagi peserta didik. (2)menganalisis kendala dan pengaruh positif pembelajaran matematika pasca pandemi praktis dan menyenangkan berbasis smart solution bagi peserta didik. (3)menganalisis respon siswa terhadap pembelajaran matematika pasca pandemi praktis dan menyenangkan berbasis smart solution bagi peserta didik. Penelitian ini menggunakan metode penelitain deskriptif kualitatif, melalui angket kepada peserta didik dan wawancara langsung dengan guru matematika harapannya bisa melihat langsung pembelajaran praktis dan menyenangkan matematika berbasis smart solution. Data pertama diambil dari pengamatan, data kedua dimabil dengan wawancara dengan guru, data yang ketiga (respon) diambil dengan angket. Tehnik analisis data menggunakan tehnik deskriptif kualitatif. Hasil penelitian menunjukkan smart solution terdiri dari dua fase, yaitu memberikan pembelajaran dengan sederhana dilanjutkan dengan pembelajaran yang menyenangkan berbasis smart solution. Kendalanya belum semua guru menyampaikan materi secara sistematis. Dengan smart solution anak-anak lebih senang belajar matematika. Respon siswa sangat menyukai pembelajaran matematika praktis dan menyenangkan berbasis smart solution.
Optimalisasi Pembelajaran Trigonometri Melalui Kombinasi Geogebra dan Infografis: Pendekatan yang Dinamis dan Menarik
Authors: Afifah Hasna 
Published: December 2024

Trigonometry is a branch of mathematics that studies the relationship between angles and sides of triangles. It has many applications in various fields, including physics, engineering, and astronomy.Trigonometry learning is often considered difficult and abstract by students. This is due to several factors, including: the abstract nature of trigonometric concepts and lack of interesting and interactive learning media. One way to overcome this is to use a dynamic and engaging approach. One approach that can be used is to integrate Geogebra and infographics in trigonometry learning. Geogebra is a mathematical software that can be used to create graphs, animations, and simulations. Geogebra can be used to visualize trigonometric concepts dynamically. Infographics are data visualizations that use text, graphs, and images to present information in an engaging and easy-to-understand way. Infographics can be used to explain trigonometric concepts more clearly. Here are some of the benefits of combining Geogebra and infographics in trigonometry learning: improves conceptual understanding, improves problem-solving skills, and increases motivation to learn. A dynamic and engaging approach can make trigonometry learning more interesting and enjoyable. This can help students to be more motivated to learn and understand trigonometric concepts better.
Keywords: trigonometry, Geogebra, infographics, dynamic learning, engaging learning