bluuebunny/crossref_metadata_2025_split huggingface dataset:
PSVIII-20 Heterologous lactobacilli feed additive impact on microflora in Cobb-500 broiler laying hens
Authors: Kurilova Anastasiia A , Karpenko Larisa Y , Balykina Anna B , Bakhta Alesya A , Kochish Ivan I , Nikonov Ilia N , Kozitcyna Anna I 
Published: October 2021

Abstract
Experiment was carried out on introducing eggshells from an experimental group of laying hens of lactobacilli strains with anti-C.jejuni activity. Eggs were incubated for 21 days to obtain broiler chickens containing auto-strains of lactobacilli with anti-C.jejuni activity in the blind processes of the intestine. It should be noted that most of the incubation eggs were observed in the 2nd group, in which the eggs were laid for subsequent administration of probiotic bacteria. Check was carried out for the presence of auto-strains of lactobacilli with anti-C.jejuni activity in the intestines of broiler chickens and the timing of their persistence for 1–35 days. The objective of the study was a comparative analysis of the following DNA samples: DNA from a feed supplement containing strains of lactobacilli with anti-C.jejuni activity; DNA samples from litter of laying hens of the parent herd receiving heterologous lactobacilli as part of the feed; isolated DNA from washes from hatching eggs; DNA samples from the contents of the digestive tract of chicken embryos; DNA samples from the intestines of broiler chickens. DNA samples from groups of birds and their embryos obtained without the use of heterologous lactobacilli were used as controls. The microflora of the contents of blind processes was taken from chickens on the 22nd day and on the 35th day of growing. In total, 343 different bacterial OTUs were identified in this study, belonging to 13 types, 26 classes, 51 orders, 106 families and 172 genera. Assessment of the biodiversity of bacterial ensembles in the samples of the contents of blind processes of chickens revealed fundamental differences already at the level of growing time and type. The study was performed at the FGBOU VO “St. Petersburg State University of Veterinary Medicine» with the aid of the Russian Science Foundation Grant (Project No. 18-76-10017).
PSXIII-16 Supplementing organic-complexed or inorganic Co, Cu, Mn, and Zn to beef cows during gestation: physiological and productive responses of cows and their offspring until weaning
Authors: Harvey Kelsey , Cooke Reinaldo F , Colombo Eduardo , Rett Bruna , Sousa Osvaldo , Harvey Lorin , Russell Jason , Pohler Ky G , Brandão Alice 
Published: October 2021

Abstract
Pregnant, non-lactating beef cows (n = 190; 117 ± 2.2 d of gestation; d 0) were assigned to receive: 1) sulfate sources of Cu, Co, Mn, and Zn (INR; n = 95) or 2) an organic complexed source of Cu, Mn, Co, and Zn (AAC; Availa®4, Zinpro; n = 95). From d 0 to calving, cows were maintained in a single pasture and individually offered treatments three times weekly. Cow BW and BCS were recorded on d -30, d 97, upon calving, and at weaning and liver biopsies performed in 30 cows/treatment. Milk production was estimated at 42 ± 0.5 d postpartum via weigh-suckle-weigh (WSW). Liver and longissimus muscle (LM) biopsies were performed in 30 calves/treatment upon calving and 24 h later, at WSW, and at weaning. No treatment effects were detected (P ≥ 0.49) for cow BCS, despite AAC cows having greater (P = 0.04) BW on d 97. Liver Co (P &lt; 0.01) concentrations were greater for AAC compared to INR cows, and liver concentrations of Cu were greater (P = 0.02) for INR compared to AAC cows on d 97. Upon calving, INR cows had greater (P ≤ 0.01) liver Cu and Zn concentrations compared to AAC cows. Cows receiving AAC had greater (P = 0.04) hepatic mRNA expression of metallothionein 1A at calving, and their calves had greater (P = 0.04) hepatic mRNA expression of superoxide dismutase at weaning. Milk production did not differ between AAC and INR cows (P = 0.70). No treatment effects were detected (P ≥ 0.29) for mRNA expression of LM genes in calves at birth and weaning. Calf birth and weaning BW were similar (P ≥ 0.19) between treatments. Hence, supplementing AAC or INR to beef cows during the last 5 mo of gestation yielded similar cow-calf productive responses until weaning.
PSXIII-25 Quality and sensory evaluation of Xhosa goat meat subjected to different watering regimen and vitamin C supplementation
Authors: Akinmoladun Oluwakamisi F , Fon Fabian N , Mpendulo Conference , Okoh Omobola 
Published: October 2021

Abstract
Water scarcity, often accompanied by limited water intake (WI) in livestock, may result in pre-slaughter stress, thereby affecting meat quality. The study evaluated water restriction (WR) levels with or without vitamin C (VC) supplementations on Xhosa goats’ performance, quality and sensory attributes of its meat. Goats (n = 42; mean weight= 15.92±2.12 kg) were distributed into seven treatments groups: without WR (W0, control), WR of 70% (W70) and 50% (W50) of ad libitum WI, WR of 70% (W70+) and 50% (W50+) of ad libitum WI plus 3g VC daily, and WR of 70% (W70++) and 50% (W50++) of ad libitum WI plus 3g VC and extra 5gVC given every eight-day.VC was administered per os. Body weight changes were taken weekly. At the end of the trial (75-d), slaughtering was done following standard procedures. pH24 was measured in the Longissimus lumborum (LTL) muscle between the 12th and 13th ribs, 24 h post-slaughter. Four pieces (2 cm thick) were cut from the carcass’s LTL muscle and assigned for thaw loss, moisture, TBARS, sensory attributes (colour, texture, taste-odour, acceptability), fat and fat-free dry matter determinations. The sensory scores were transformed to achieve normality. Data obtained were analyzed using the GLM of SAS (2003). The dry matter intake and body weight decreased (P ≤ 0.05) in response to WR levels and rose in the vitamin C treated groups. Effect of WR levels and VC supplementation were not significant (P &gt; 0.05) on TBARS, pH24, colour, odour and thaw loss. The meat appearance, moisture and fat content decreased (P ≤ 0.05) with WR levels. This study demonstrates that reduced body weight due to suboptimal WI could be lessened with daily VC supplementation. Also, WR levels and with or without VC, did not impact negatively on the meat quality parameters like oxidative stability of lipids, thaw loss and pH24
PSXIII-18 The effect of lead on alkaline phosphatase serum activity in European carp
Authors: Bokhan Polina D , Polistovskaia Polina A , Karpenko Larisa Y , Enukashvili Abram I , Bakhta Alesya A , Kozitcyna Anna I 
Published: October 2021

Abstract
The aim of this research is to assess the toxic influence of heavy metals in fish. This study was intended to analyze alkaline phosphatase (AP) serum activity under the influence of various lead concentrations in European carp. AP is involved in the dephosphorylation processes and is an important indicator of liver and skeleton tissue metabolism. When exposed to heavy metals, a change in the activity of this enzyme can be observed. In this experiment 3 groups of fish (European carp) were formed, 10 fish in each group: control group; 1-experimental group – fish were contained in water with 0.06 mg/l lead acetate concentration (upper threshold level than fishery waters permissible exposure limit according to the laws of the Russian Federation); 2-experimental group – fish were contained in water with 0.6 mg/l lead acetate concentration (10 times higher than fishery waters permissible exposure limit according to the laws of the Russian Federation). Exposure time was 4 hours. The blood samplings were taken from heart by syringe and placed in dry test tubes. After samplings centrifugation in non-hemolysed serum samples AP activity was assessed. The study revealed the decreasing trend of AP serum activity by 1.08% (21.89±0.24 IU/l) in 1-experimental group in compare with control group (22.13±0.78 IU/l). In case where the lead acetate concentration were 10 times higher than fishery waters permissible exposure limit were meaningful decreasing of AP activity (P ≤ 0.05) by 66.23% (17.42±0.63 IU/l) accordingly in 2-experimental group in compare with control group. In conclusion, a statistically significant decrease in the AP activity in fish blood serum was found after acetate concentration 10 times higher than the permissible exposure limit in fishery waters, according to the laws of the Russian Federation. We can suppose that the activity of this enzyme is the reason for the inhibitory effect of lead on liver tissue.
PSXIII-26 Supplementation with a polyphenolic pine bark extract reduces ammonia nitrogen production in a RUSITEC system as determined by principal component analysis
Authors: Vera Nelson , Gutierrez Constanza , Williams Pamela , Fuentealba Cecilia , Allende Rodrigo , Avila-Stagno Jorge 
Published: October 2021

Abstract
The aim of the study was to correlate the effects of supplementation with a polyphenolic pine bark extract (PBE) in diets with different forage to concentrate (F:C) ratio on methane (CH4), ammonia nitrogen (NH3–N) production and ruminal fermentation parameters using the Rumen Simulation Technique (RUSITEC). The experimental diets were F:C 70:30 (HF) or F:C 30:70 (HC) with or without 2% PBE on a DM basis. The four diets were isoproteic (15% CP), with similar OM (HF 94% and HC 96%), but different NDF (HF 40% and HC 25%). The treatments, in duplicate, were assigned in an 8 fermenter RUSITEC apparatus. Incubations were run twice, with 5 days of sampling after 10 days adaptation. The experimental design was a 2x2 factorial arrangement in a randomized complete block with repeated measures. Pearson correlation and principal component analysis (PCA) were conducted to elucidate relationships among PBE total polyphenols (TP) and the variables evaluated. The TP was highly correlated with NH3–N (r = –0.98; P &lt; 0.001) and butyrate (r = –0.85; P &lt; 0.001), and had a high correlation with propionate (r = 0.75; P &lt; 0.001) and acetate (r = 0.68; P = 0.001). Correlation with total VFA was moderate (r = –0.59; P = 0.006), and CH4 yield and IVDMD there were not correlated (r ≤ –0.07; P ≥ 0.188). The PCA (KMO = 0.655; BTS &lt; 0.001) shows that 75.2% of the total variation is explained by the first two principal components (PC1 = 46.5% and PC2 = 28.7%). In the score plot, PC1 discriminated between diets with and without PBE, while the PC2 separated based on NDF. The loading plot showed that TP and propionate were clustered, and had inverse directions to NH3–N. In conclusion, the PBE supplementation reduces NH3–N production in a RUSITEC system without decreasing CH4 yield or negatively affecting ruminal fermentation parameters.
PSXIII-22 Heat stress in north central Mexico and time of exposure to it, measured by the Environmental Temperature Index
Authors: Rodriguez-Martinez Rafael , Meza-Herrera César A , Rodriguez-Venegas Rafael , Robles-Trillo Pedro Antonio , Rivas-Madero Jesús Salvador 
Published: October 2021

Abstract
The Temperature Humidity Index (THI) is the most used to identify the risk of heat stress for dairy cattle, which is based on the environmental temperature (ºC) and humidity (%), and of which they are currently considered 68 points as the threshold for high producing dairy cows. In the Comarca Lagunera, located in north central Mexico, 21% of the Mexican dairy production is generated annually, despite its environmental conditions characterized by temperatures up to 41.5 ° C in the summer, low relative humidity and high solar radiation. The objective was to identify the average number of days in which a THI ≥68 is reached per month in each season of the year with 1, 8 and 16 h of exposure in dairy farms of the region, for which, the information recorded every 10 min was analyzed daily in five farms for 6 years (2015–2020), using the DiGiTH ™ application (DiGiTH Technologies, Mexico) and through ANOVA (proc GLM; SAS Inst. Inc., Cary, NC, USA), to identify differences seasonal for this variable. The attached table shows that spring and summer were the seasons in which the highest number of average days per month were recorded, and winter in which the fewest days were recorded. Regarding the hours of exposure, it is observed that spring and summer are very similar in 1 and 8 hours of exposure, while for autumn and winter a clearer decrease is observed according to the hours of exposure for these seasons. These results indicate a very marked seasonal variability and in terms of the hours of exposure, which should be considered in order to adopt the measures to reduce the negative effects for the dairy cattle of the region.
PSIX-2 Carcass traits of nelore bulls: Supplementation with probiotic (Saccharomyces cerevisiae boulardii CNCM I-1079) during adaptation in feedlot
Authors: Bomfim Lorena Emily L M , Nascimento Kaique S , Moreira Couto Victor R , Arnhold Emmanuel , da R Fernandes Márcia Helena M , Moura Silva Luan O , Mari Lucas José , Santos Mateus Castilho , Gauthier Marine , Chevaux Eric , De Resende Fernandes Juliano José 
Published: October 2021

Abstract
This study aimed to evaluate the effect of probiotic (Saccharomyces cerevisiae boulardii CNCM I-1079) supplementation during feedlot adaptation period on performance of Nellore bulls. One-hundred ninety-eight Nellore bulls were used in a completely randomized block design, divided in 22 pens (9 animals/pen). Treatments were: CON – adaptation diet and finishing diet comprised of basal diet (with 28 ppm of monensin); and SCB – same diets as for the previous treatment with monensin plus probiotic (Saccharomyces cerevisiae boulardii CNCM I-1079, ProTernative® 20; Lallemand Animal Nutrition at a dose of 1.0 x 10e10 cfu/animal/d) supplementation. Supplementation was offered for the first 42 days (21 days in the step-up phase and 21 days in the finishing diet with 900 g concentrate/kg DM), then both treatments were fed the same basal diet for another 76d, reaching 118 days in total. Pen was considered the experimental unit and data were analyzed using the MIXED procedure of SAS with Tukey test at 5% significance level. Final carcass weight was 1.69% greater for animals fed SCB (283.8 vs. 288.7 kg, P = 0.04). Total carcass weight gain (111.0 vs. 114.8 kg) and daily carcass weight gain (0.924 vs. 0.956 kg) tended (P = 0.06) to increase by 3.46% in SCB-fed animals compared to the CON ones. Gain yield (P = 0.39), biological efficiency (P = 0.35) and carcass yield (P = 0.71) did not differ between treatments. Probiotic supplementation with live yeast during the first 42 days in feedlot helped Nellore bulls optimize early on growth performance with an interesting carry-over effect on carcass gain through the total feedlot time.
PSVI-30 Feedlot performance and carcass characteristics of beef steers under intensive management with or without an added nutritional packet
Authors: Osorio Andrea M , Nardi Kaue Tonelli , Fávero Igor Gomes , Scaranto Silva Kaliu G , Coello Kymberly D , Raudales Angel A , Ciriaco Francine M , Sarturi Jhones Onorino , Henry Darren D 
Published: October 2021

Abstract
The effects of a nutritional packet on performance and carcass characteristics of feedlot beef steers were evaluated. Thirty Angus-crossbred steers (BW = 542 ± 8.4 kg) were used in a randomized complete block design and allocated into pens with SmartFeed (C-Lock; 15 steers/treatment). Steers were consuming a steam-flaked corn-based finishing-diet, ad libitum, for the last 65-d on feed prior to slaughter, in which treatments were: 1) control and 2) a nutritional-packet [0.29% DM basis; live yeast (Saccharomyces cerevisiae; 8.7 Log CFU/g); Vitamin C (5.4 g/kg); Vitamin B1 (13.33 g/kg); NaCl (80 g/kg); KCl (80 g/kg)]. Individual BW was recorded every 14 d and carcass quality data was collected at slaughter. Steer was considered the experimental unit. The MIXED procedure of SAS was used with the fixed effect of treatment and the random effect of block. Total DMI was unaffected (P = 0.610) by treatment from d 0–65; however, control steers had greater (P = 0.030) DMI from d 54–65. The G:F was unaffected (P = 0.830) by treatment from d 0–35; however, it was greater (P ≤ 0.001) for steers fed the nutritional packet from d 0–50, and 0–65. Treatment did not affect (P = 0.920) ADG from d 0–35, however it increased (P ≤ 0.001) ADG from d 0–50 and tended (P = 0.060) to increase ADG from d 0–65. Carcass adjusted final BW tended (P = 0.060) to increase by 28% for treated cattle, whereas the Longissimus dorsi area tended to decrease (P = 0.090). No differences (P = 0.240) were observed for fat thickness, while yield-grade (P = 0.03) increased for treated cattle. The nutritional packet appears to improve performance in the final days of feeding steers under intensive management, and energy partitioning may be altered to fat deposition.
PSVII-18 Association of post-absorptive glucose and acetate metabolism with feed intake, growth, and efficiency in finishing beef heifers
Authors: Foote Andrew P , Rathert Abigail R , Salisbury Carlee M , McConnell Hunter L , Lalman David 
Published: October 2021

Abstract
Glucose and acetate are important nutrients for muscle and fat accretion in beef cattle. The objective of this experiment was to determine if the demand for acetate and glucose, as well as insulin response to glucose, are associated with dry matter intake (DMI), average daily gain (ADG), residual feed intake (RFI), and gain:feed (G:F). Charolais heifers (n = 16; initial BW = 412 ± 10 kg) were trained to close human contact and fed a finishing diet ad libitum in an Insentec feeding system. Following a 12-hour fast, a jugular catheter was inserted, and an acetate clearance test was performed by infusing acetate (2.18 mmol/kg BW0.75) and collecting blood samples over a 30-minute period. One hour after the conclusion of the acetate test, a glucose clearance test was performed by infusing glucose (7.57 mmol/kg BW0.75) and collecting samples over a two-hour period. Four days after the metabolic tests, heifers began an 84-d DMI and ADG test period. The area under the acetate, glucose, and insulin curves were calculated as were the clearance rate, peaks, nadir, and insulin time to peak. Pearson correlations were calculated for the metabolic parameters and production traits using SAS 9.4. Heifers gained 1.69 ± 0.03 kg/d and consumed 10.4 ± 0.19 kg/d. Acetate and glucose clearance rates were not associated with any production trait (P &gt; 0.40). Insulin time to peak concentration after the glucose challenge was associated (r = 0.69; P = 0.003) with G:F, but peak concentration was not (P = 0.45). Additionally, there was a trend (r = 0.40; P = 0.13) for area under the insulin curve to be associated with G:F. Given the small sample size in this experiment, it is possible that decreased insulin sensitivity early in the finishing period is related to improved feed efficiency in finishing heifers.
PSVII-13 Performance and environmental benefits from biochar supplementation in beef cattle grazing systems
Authors: Conlin Emily , Lardner Herbert , Ellis Jennifer L , Mandell Ira B , Wood Katharine M 
Published: October 2021

Abstract
Worldwide, beef production systems represent a significant source of greenhouse gas (GHG), and enteric methane (CH4) emissions are the primary concern. The objective of this experiment was to determine whether biochar (Oregon Biochar Solution, White City, OR) supplementation can reduce CH4 emissions from grazing beef cows. Biochar is a stable form of carbon produced through the pyrolysis of organic matter (typically forestry waste). Sixty-four cows and their calves were blocked by cow body weight and calf age, and randomly allocated to 8 paddocks, each with 8 cow-calf pairs. Using a crossover design, each paddock was assigned to one of two treatments: (1) biochar supplemented at approximately 3% of estimated dry matter intake (DMI) or (2) control (no biochar). Biochar was incorporated into a pellet containing 45% biochar, 42.5% wheat midds, 10% canola oil, and 2.5% dry molasses and fed in a portable trough once daily. Each period consisted of 28 days: 21 days for biochar adaptation and 7 days for data collection. Enteric gas emissions from each paddock were measured using C-Lock GreenFeed trailers (C-Lock Inc., Rapid City, SD, USA) with pasture DMI estimated using paddock entry/exit quadrats during each sampling week. Enteric CH4 emissions expressed as g CH4/d were 249 and 260 ± 50.3 g (P ≥ 0.37) for control and biochar, respectively. Similarly, g CH4/kg DM and g CH4/kg BW were not affected (P ≥ 0.44) by biochar supplementation on pasture. Biochar supplementation did not affect estimated DMI or cow/calf body weights (P ≥ 0.15). Results suggest that biochar was ineffective for reducing methane emissions from grazing beef cows; however, measures of animal performance were not affected by biochar consumption. Further work is required to determine if type or higher inclusions of biochar can reduce CH4 emissions from beef cattle.
PSX-B-10 Effect of undigested neutral detergent fiber concentration and forage inclusion rate on ruminal pH, reticular motility, and total tract permeability for finishing beef heifers
Authors: Pereira Murillo C , Beauchemin Karen A , McAllister Tim A , Yang Wenzhu , Van Donkersgoed Joyce , Gibb Darryl , Wood Katharine M , Penner Gregory B 
Published: October 2021

Abstract
This study evaluated the effects of undigested neutral detergent fiber (uNDF) concentration and forage inclusion (FI) rate on dry matter (DM) intake, ruminal pH, reticular contractions, and gastrointestinal permeability for finishing beef cattle. Five ruminally cannulated Hereford′Simmental heifers (699±69.1 kg) were used in an incomplete 6×6 Latin square (26-d periods) with a 2×3 factorial treatment arrangement. Barley grain-based diets were formulated using barley silage or wheat straw to provide low or high uNDF (7.1 vs. 8.5% DM) with forage proportions of 5, 10, or 15% of dietary DM. Dry matter intake (P ≥ 0.10) and eating time (P ≥ 0.13) were not affected by uNDF, FI, or uNDF′FI. With low uNDF diets, increasing FI numerically (P = 0.02) increased rumination time (min/d); while, with high uNDF diets, rumination time increased with 5 to 10% FI, but not thereafter (P = 0.03). Mean ruminal pH was not affected by uNDF (6.17 vs. 6.19; P = 0.08), but increased with increasing FI (6.04b, 6.23a, and 6.28a; P = 0.02). Duration of ruminal pH &lt; 5.5 was not affected by uNDF but tended (P = 0.07) to be reduced with increasing FI. High uNDF diet tended to increase the frequency of reticular contractions (1.43 vs. 1.51 contractions/min; P = 0.07) but decreased the contraction duration (13.2 vs. 14.1 sec; P = 0.04). Increasing FI increased contraction frequency (1.39b, 1.50a, and 1.53a contractions/min; P = 0.03) and tended to reduce contraction duration as forage increased from 5 to 10 and 15% (14.3, 13.1, and 13.6 sec; P = 0.07). Feeding high uNDF decreased (P = 0.05) permeability of the gastrointestinal tract based on the appearance of Cr-EDTA in urine following an intra-ruminal dose. Increasing FI tended to reduce gastrointestinal tract permeability (P = 0.06). Limited interactions indicate that uNDF and FI act independently suggesting that increasing dietary uNDF, without increasing FI rate, can stimulate frequency of reticulo-ruminal contractions and reduce gastrointestinal permeability for finishing cattle.