bluuebunny/crossref_metadata_2025_split huggingface dataset:
Pathological significance and prognostic role of LATS2 in prostate cancer
Authors: Matsuda Tsuyoshi , Miyata Yasuyoshi , Nakamura Yuichiro , Otsubo Asato , Mukae Yuta , Harada Junki , Mitsunari Kensuke , Matsuo Tomohiro , Ohba Kojiro , Furusato Bungo , Sakai Hideki 
Published: September 2021

AbstractBackgroundLarge tumor suppressor 2 (LATS2) is an important regulator of the Hippo pathway and it plays crucial roles in cell survival and behaviors. Herein, we evaluated the pathological roles of LATS2 in prostate cancer (PC), for which very little information is available.MethodsCell proliferation, migration, and invasion in response to the siRNA‐mediated knockdown (KD) LATS2 expression were evaluated in two PC cell lines (LNCaP and PC3). The expression of LATS2 in specimens from 204 PC patients was investigated immunohistochemically, and the relationships between its expression and clinicopathological features, proliferation index (PI; measured using an anti‐KI‐67 antibody), and biochemical recurrence (BCR) were investigated.ResultsKD of LATS2 increased the growth, migration, and invasion in LNCaP cells and only increased migration in PC3 cells. The expression of LATS2 was negatively associated with the grade group, T, N, M stage, and PI. In addition, the expression of LATS2 was a useful predictor of the histological effects of neoadjuvant hormonal therapy and BCR‐free survival periods. A multivariate analysis model including clinicopathological features showed that negative expression of LATS2 had a significantly higher risk of BCR (odds ratio = 2.95, P < 0.001).ConclusionsLATS2 acts as a tumor suppressor in PC. LATS2 expression is a useful predictor for BCR. LATS2‐related activities are possibly dependent on the androgen‐dependency of PC cells. Therefore, we suggest that LATS2 could be a potential therapeutic target and a useful predictor for outcome in patients with PC.
An automated streamlined method for the therapeutic drug monitoring of digoxin based on the online‐solid‐phase extraction liquid chromatography tandem high‐resolution mass spectrometry
Authors: Liu Congmin , Wang Li , Pan Lixuan , Hou Wenqing , Hao Zhihui , Qu Lihua 
Published: September 2021

RationaleDigoxin is widely used in the clinical treatment of cardiovascular diseases. However, due to its extremely narrow therapeutic window, therapeutic drug monitoring (TDM) is vitally important. In consideration of the time‐consuming and labor‐intensive nature of the traditional techniques, an automated and efficient method was required for the clinical individualized TDM of digoxin.MethodsAn online solid‐phase extraction liquid chromatography tandem high‐resolution mass spectrometry (online‐SPE‐LC‐HRMS) method was developed and applied for the determination of digoxin in plasma. The online SPE‐LC steps included pretreatment and separation of plasma samples that were carried out using a Waters Oasis HLB cartridge and XBridge Shield RP18 column, respectively. A high‐resolution Q Orbitrap mass spectrometer with targeted‐selected ion monitoring in negative scan mode was applied to monitor formate‐adduct ions [M + HCOO]− m/z 825.42781 for digoxin.ResultsLinearity was shown over the range 0.1–10 ng mL−1 for digoxin with correlation coefficients of R2 > 0.999. The lower limit of quantitation (LLOQ) for digoxin was 0.1 ng mL−1. Extraction recoveries ranged from 82.61% to 94.28% for digoxin. The intra‐ and inter‐day precision values were < 5.53% with accuracy ranging from 84.97% to 96.75%. The total running time was 10 min for each sample.ConclusionThe established method displayed satisfactory recoveries, accuracy, precision, and stability, and successfully applied on the TDM of digoxin. This automated streamlined method provides a powerful tool to guide the individualized administration of digoxin, which is significant for the practice of precision medicine.
Developing a taxa‐related physical habitat score based on the response of macroinvertebrate community structure to fine bed sediment composition in the Tsitsa River, Eastern Cape, South Africa
Authors: Huchzermeyer Nicholaus H. , Rowntree Kate 
Published: September 2021

AbstractBiomonitoring techniques such as the South African Scoring System version 5 (SASSv5) is widely used in South Africa to rapidly assess river water quality, but there are currently no equivalent rapid monitoring tools for fine sediment assessments. This article presents a tool that monitors the effects of fine sediment on macroinvertebrates that can be used alongside the SASSv5 assessment. Sampling was carried out in the Tsitsa River in the Eastern Cape province of South Africa. Five sampling surveys were conducted over one hydrological year to capture a range of flow conditions resulting in a total of 90 sampling points across five sampling sites. Using an adaption of the SASSv5 methods, sampling was undertaken at a quadrat scale, rather than at a site scale, to identify macroinvertebrate families in different habitat types and degrees of sediment deposition. Fine sediment sampling using a disturbance technique was undertaken immediately upstream of 78 of the biological sampling points. A taxa‐related sediment deposition score was created in which taxa were scored in terms of their sensitivity to fine sediment accumulation. The index was tested as a monitoring tool at 11 monitoring sites along the Tsitsa River in different seasons and flow conditions in 2019 and 2020.
Genetic diversity assessment of Georgia peanut cultivars developed during ninety years of breeding
Authors: Brown Nino , Branch William D. , Johnson Matthew , Wallace Jason 
Published: September 2021

AbstractThe cultivated peanut(Arachis hypogaea L.) has experienced severe genetic bottlenecks over the course of its evolution and domestication. Most genetic diversity studies in peanut have focused on global genetic stocks, wild accessions, and related species, but few have focused on elite cultivars. The objective of this project was to assess the genetic diversity of 32 peanut cultivars developed by the University of Georgia breeding program since its inception in 1931. Quantifying genetic similarity (GSIM) among these cultivars will provide a better understanding of their relationships and aid in breeding for cultivar development. Genotyping‐by‐sequencing (GBS), in concert with the recently published A. hypogaea genome sequence, was used to identify a total of 27,142 single nucleotide polymorphisms (SNPs) among these cultivars. Coefficients of parentage (CoP) were calculated based on publicly available pedigree information and compared with SNP‐based GSIM estimates; the resulting correlations were low, ranging from R2 = 0.212–0.279. Although genetic diversity is generally low in cultivated peanut, our data indicate that the genetic diversity of Georgia cultivars has actually increased since the early days of the breeding program, likely a result of the incorporation of diverse germplasm and breeding lines into the program. The results reported here provide a valuable understanding of genetic variation among elite Georgia peanut cultivars that have had a significant impact on the peanut industry within the United States.
Small Changes that Make a Great Difference: Reading, Playing and Eating with your Children and the Facilitating Role of Managers in Latin America
Authors: Bosch María José , Las Heras Mireia 
Published: September 2021

AbstractParenting is challenging in today’s world. Dual careers, hyper-connectivity, and long distances take almost all our time, and parents must integrate their different roles. A direct impact of this hectic life is on the time parents spend with their children. Additionally, the role of fathers has gained importance, and it is important to understand his influence. In this chapter we will analyze the importance of the time fathers spend in positive engagement activities with their children, such as eating and reading with their children, and also how organizations, through their managers, can promote these positive engagement activities. Also, to show how context influences this relationship, we compare different countries in Latin America: Argentina, Chile, Colombia, El Salvador, Guatemala, Mexico, and the Dominican Republic.
Fatherhood and Reproductive Health in the Antenatal Period: From Men’s Voices to Clinical Practice
Authors: Levy Raymond A. , Kotelchuck Milton 
Published: September 2021

AbstractThere is very limited literature on the experiences of fathers during Obstetric prenatal care (PNC), especially hearing from fathers’ voices directly. The MGH Fatherhood Project conducted two annual surveys—data combined for analysis—of all fathers who accompanied their partners to prenatal care visits over 2-week periods at a large, tertiary-care urban hospital in Boston, MA. The anonymous, voluntary close-ended survey was offered in multiple languages and self-administered on iPads.Results: Nine hundred fifty nine fathers participated, 86% of attending fathers, possibly making the study the largest research sample of fathers in PNC. Fathers are actively and deeply engaged with the impending birth; they have substantial physical health needs (obesity, family planning and lack of primary care), and mental health needs (stress, depressive symptoms, and personal isolation). Fathers perceived they were well treated during the PNC visit, but were desirous of more reproductive, relational, and infant health information and skills, which they preferred to receive from publications, social media, or health professionals; and they were very supportive of PNC fatherhood initiatives.Discussion: The results suggest five sets of practical recommendations to create a more father-friendly environment in Obstetric care-Staff Training; Father-Friendly Clinic Environment; Explicit Affirmation of Father Inclusion; Development of Educational Materials; and Specialized Father-Focused Health Initiatives, all with the goal of improving reproductive health outcomes for families.
Bildgebung in der prächirurgischen Epilepsiediagnostik
Authors: Ilyas-Feldmann Maria , Vorderwülbecke Bernd , Steinbrenner Mirja 
Published: September 2021

ZusammenfassungWährend zwei Drittel der PatientInnen mit Epilepsie durch Medikamente anfallsfrei werden, ist die Erkrankung bei 30 % pharmakoresistent. Bei pharmakoresistenter fokaler Epilepsie bietet die Epilepsiechirurgie eine etwa 65 %ige Chance auf Anfallsfreiheit. Vorab muss der Anfallsfokus exakt eingegrenzt werden, wofür bildgebende Methoden unverzichtbar sind. In den letzten Jahren hat sich in der Prächirurgie der Anteil von PatientInnen mit unauffälliger konventioneller Magnetresonanztomographie (MRT) erhöht. Allerdings konnte die Sensitivität der MRT durch spezielle Aufnahmesequenzen und Techniken der Postprozessierung gesteigert werden. Die Quellenlokalisation des Signals von Elektro- und Magnetenzephalographie (EEG und MEG) verortet den Ursprung iktaler und interiktaler epileptischer Aktivität im Gehirn. Nuklearmedizinische Untersuchungen wie die interiktale Positronen-Emissions-Tomographie (PET) und die iktale Einzelphotonen-Emissionscomputertomographie (SPECT) detektieren chronische oder akute anfallsbezogene Veränderungen des Hirnmetabolismus und können auch bei nichtlokalisierendem MRT auf den epileptogenen Fokus hinweisen. Alle Befunde zusammengenommen werden zur Planung eventueller invasiver EEG-Ableitungen und letztlich der chirurgischen Operation eingesetzt. Konkordante Befunde sind mit besseren chirurgischen Ergebnissen assoziiert und zeigen auch im Langzeitverlauf signifikant höhere Anfallsfreiheitsraten.
Risk factors and incidence over time for lower extremity amputations in people with type 1 diabetes: an observational cohort study of 46,088 patients from the Swedish National Diabetes Registry
Authors: Hallström Sara , Svensson Ann-Marie , Pivodic Aldina , Ólafsdóttir Arndís F. , Löndahl Magnus , Wedel Hans , Lind Marcus 
Published: September 2021

Abstract
Aims/hypothesis
The aim of this work was to study the incidence over time of lower extremity amputations and determine variables associated with increased risk of amputations in people with type 1 diabetes.

Methods
Individuals with type 1 diabetes registered in the Swedish National Diabetes Registry with no previous amputation from 1 January 1998 and followed to 2 October 2019 were included. Time-updated Cox regression and gradient of risk per SD were used to evaluate the impact of risk factors on the incidence of amputation. Age- and sex-adjusted incidences were estimated over time.

Results
Of 46,088 people with type 1 diabetes with no previous amputation (mean age 32.5 years [SD 14.5], 25,354 [55%] male sex), 1519 (3.3%) underwent amputation. Median follow-up was 12.4 years. The standardised incidence for any amputation in 1998–2001 was 2.84 (95% CI 2.32, 3.36) per 1000 person-years and decreased to 1.64 (95% CI 1.38, 1.90) per 1000 person-years in 2017–2019. The incidence for minor and major amputations showed a similar pattern. Hyperglycaemia and renal dysfunction were the strongest risk factors for amputation, followed by older age, male sex, cardiovascular comorbidities, smoking and hypertension. Glycaemic control and age- and sex-adjusted renal function improved during the corresponding time period as amputations decreased.

Conclusions/interpretation
The incidence of amputation and of the most prominent risk factors for amputation, including renal dysfunction and hyperglycaemia, has improved considerably during recent years for people with type 1 diabetes. This finding has important implications for quality of life, health economics and prognosis regarding CVD, indicating a trend shift in the treatment of type 1 diabetes.

Graphical abstract
Increased patellar bone tracer uptake in preoperative SPECT/CT before medial opening high tibial osteotomy correlates with inferior clinical outcome
Authors: Schelker B. L. , Moret C. S. , Dogan O. , Amsler F. , Rasch H. , Hügli R. W. , Hirschmann M. T. 
Published: September 2021

Abstract
Purpose
The purpose of this study was to investigate whether specific bone tracer uptake (BTU) patterns on preoperative SPECT/CT could predict which patients with varus alignment and medial overload would particularly benefit from medial opening-wedge high tibial osteotomy (MOWHTO). It was the hypothesis that an increased preoperative BTU relative to the reference BTU of the femur on SPECT/CT in the lateral and patellar compartments of the knee are predictive factors for inferior clinical outcome and that the clinical outcome correlates with the extent of alignment correction.

Methods
Twenty-three knees from 22 patients who underwent MOWHTO for medial compartment overload were investigated preoperatively using Tc-99m-SPECT/CT. BTU was quantified and localised to specific joint areas according to a previously validated scheme. Pre- and postoperative mechanical alignment was measured. Clinical outcome was assessed at a median of 24 months (range 11–30) after MOWHTO by collecting the WOMAC score.

Results
Significant correlations between BTU in the patellar area and the total WOMAC score and its subcategories pain and stiffness were found. Thus, BTU in the 1sPat area (superior lateral patellar compartment) correlated with total WOMAC (rho = 0.43, p = 0.04), pain subcategory (rho = 0.43, p = 0.04), and stiffness subcategory (rho = 0.59, p = 0.003). No significant correlations were found between alignment correction, age, gender and WOMAC.

Conclusion
This study highlights the role of preoperative SPECT in modern knee surgery to obtain information about the loading pattern on different compartments of the knee. Despite the limited number of participants, the present study shows that a preoperative SPECT/CT scan can help the treating surgeons to identify patients who may be at risk of inferior clinical outcome if an MOWHTO is considered, as an elevated BTU in the patellar region on preoperative SPECT/CT appears to be a potential risk factor for postoperative pain and stiffness.

Level of evidence
Level III.
Single-stage versus staged interposition urethroplasty for glandular hypospadias with severe penile curvature: 15-year experience
Authors: Zhou Guanglun , Xu Wanhua , Yin Jianchun , Sun Junjie , Yang Zhilin , Li Shoulin 
Published: September 2021

Abstract
Purpose
Our study examined the benefit of an alternative interposition urethroplasty (IU) procedure for glandular hypospadias (GH) with severe penile curvature (SPC). The technique involved transecting and reconstructing the urethra to preserve the distal glandular and coronal urethra and correct the curvature. We compared procedural characteristics, outcomes, and surgical complications for the single-stage and staged IU techniques.

Methods
We retrospectively studied 44 patients with GH with SPC who underwent single-stage or staged IU between March 2005 and June 2020. Demographics, operative details, complications, and uroflometry findings were analyzed.

Results
The median age at initial surgery was 37.5 months. Ten patients underwent single-stage IU repair, and 34 patients underwent staged IU repair. The median length of the interposition neourethra was 3.2 cm (2.2–4.3). The median follow-up duration was 58 months, and the overall complication rate was 13.6%. Complications were noted in 30% (3/10) and 8.8% (3/34) of patients in the single-stage and staged IU groups, respectively (p > 0.05). Fistula formation was noted in one and three patients in the single-stage and staged groups, respectively (8.8% vs. 10%, p > 0.05). Two cases of urethral stricture were documented in the single-stage group only. No chordee recurrence or urethral diverticula was noted in any of the patients.

Conclusion
IU is a reliable and durable technique for GH with SPC. It avoided penile shortening, preserved the distal urethra, and reduced the risk of chordee recurrence. The staged IU technique had more superior outcomes compared to the single-stage IU technique.
Symptomatic and restorative therapies in neuromyelitis optica spectrum disorders
Authors: Abboud Hesham , Salazar-Camelo Andrea , George Naveen , Planchon Sarah M. , Matiello Marcelo , Mealy Maureen A. , Goodman Andrew ,   On-behalf of the Guthy-Jackson Foundation NMO International Clinical Consortium, Alroughani Raed , Altintas Ayse , Apiwattanakul Metha , Asgari Nasrin , Paolilo Renata Barbosa , Bennett Jeffrey , Bichuetti Denis Bernardi , Blaschke Terrence F. , Boyko Alexey , Broadley Simon , Carnero Contentti Edgar , Cohen Jeffrey A. , Delgado-Garcia Guillermo , Basuroski Irena Dujmovic , Flores-Rivera Jose , Fujihara Kazuo , Havla Joachim , Hellwig Kerstin , Hor Jyh Yung , Huda Saif , Iorio Raffaele , Jarius Sven , Kimbrough Dorlan , Kister Ilya , Kleiter Ingo , Kissani Najib , Lana-Peixoto Marco , Leite Maria Isabel , Levy Michael , Maiga Youssoufa , Mao-Draayer Yang , Mariotto Sara , Melamed Esther , Neubrand Veronika E. , Oreja-Guevara Celia , Palace Jacqueline , Pröbstel Anne-Katrin , Qian Peiqing , Quan Chao , Riley Claire , Ringelstein Marius , Sa Maria Jose , Siritho Sasitorn , Smith Terry J. , de Castillo Ibis Soto , Tenembaum Silvia , Villoslada Pablo , Wuerfel Jens , Wingerchuk Dean , Yamout Bassem , Yeaman Michael 
Published: September 2021

AbstractNeuromyelitis optica spectrum disorders (NMOSD) are a group of autoimmune inflammatory conditions that primarily target the optic nerves, spinal cord, brainstem, and occasionally the cerebrum. NMOSD is characterized by recurrent attacks of visual, motor, and/or sensory dysfunction that often result in severe neurological deficits. In recent years, there has been a significant progress in relapse treatment and prevention but the residual disability per attack remains high. Although symptomatic and restorative research has been limited in NMOSD, some therapeutic approaches can be inferred from published case series and evidence from multiple sclerosis literature. In this review, we will discuss established and emerging therapeutic options for symptomatic treatment and restoration of function in NMOSD. We highlight NMOSD-specific considerations and identify potential areas for future research. The review covers pharmacologic, non-pharmacologic, and neuromodulatory approaches to neuropathic pain, tonic spasms, muscle tone abnormalities, sphincter dysfunction, motor and visual impairment, fatigue, sleep disorders, and neuropsychological symptoms. In addition, we briefly discuss remyelinating agents and mesenchymal stem cell transplantation in NMOSD.
A Modified Phase-Field Fracture Model for Simulation of Mixed Mode Brittle Fractures and Compressive Cracks in Porous Rock
Authors: Spetz Alex , Denzer Ralf , Tudisco Erika , Dahlblom Ola 
Published: September 2021

AbstractIn this work, we propose a modified phase-field model for simulating the evolution of mixed mode fractures and compressive driven fractures in porous artificial rocks. For the purpose of validation, the behaviour of artificial rock samples, with either a single or double saw cuts, under uniaxial plane strain compression has been numerically simulated. The simulated results are compared to experimental data, both qualitatively and quantitatively. It is shown that the proposed model is able to capture the commonly observed propagation pattern of wing cracks emergence followed by secondary cracks driven by compressive stresses. Additionally, the typical types of complex crack patterns observed in experimental tests are successfully reproduced, as well as the critical loads.
Differential metabolism-associated gene expression of duck pancreatic cells in response to two strains of duck hepatitis A virus type 1
Authors: Chen Zhen , Shi Shao-hua , Huang Yu , Huang Cui-qin , Liu Rong-chang , Cheng Long-fei , Fu Guang-hua , Chen Hong-mei , Wan Chun-he , Fu Qiu-ling 
Published: September 2021

AbstractSeveral outbreaks of duck hepatitis A virus type 1 (DHAV-1), which were characterized by yellow coloration and hemorrhage in pancreatic tissues, have occurred in China. The causative agent is called pancreatitis-associated DHAV-1. The mechanisms involved in pancreatitis-associated DHAV-1 infection are still unclear. Transcriptome analysis of duck pancreas infected with classical-type DHAV-1 and pancreatitis-associated DHAV-1 was carried out. Deep sequencing with Illumina-Solexa resulted in a total of 53.9 Gb of clean data from the cDNA library of the pancreas, and a total of 29,597 unigenes with an average length of 993.43 bp were generated by de novo sequence assembly. The expression levels of D-3-phosphoglycerate dehydrogenase, phosphoserine aminotransferase, and phosphoserine phosphatase, which are involved in glycine, serine, and threonine metabolism pathways, were significantly downregulated in ducks infected with pancreatitis-associated DHAV-1 compared with those infected with classical-type DHAV-1. These findings provide information regarding differences in expression levels of metabolism-associated genes between ducks infected with pancreatitis-associated DHAV-1 and those infected with classical-type DHAV-1, indicating that intensive metabolism disorders may contribute to the different phenotypes of DHAV-1-infection.
Corporate Social Innovation in Developing Countries
Authors: Saka-Helmhout Ayse , Chappin Maryse M. H. , Rodrigues Suzana B. 
Published: September 2021

AbstractAlthough corporate social innovation studies in developing countries acknowledge the importance of firm resources and capabilities for attaining social goals, they overlook the way in which these interact with broader institutions to generate successful outcomes. We address this gap by exploring the relationship between firm resources-capabilities and institutions that is conducive to meeting both business and social interests in developing countries. By employing a fuzzy-set qualitative comparative analysis of corporate social innovation projects performed by joint ventures of Dutch SMEs and their local partners in developing countries, we show that firm resources and/or capabilities complement strong institutions in these countries. Corporate social innovation can also be facilitated by firm capabilities in running highly legitimate projects that substitute institutional voids in these economies, attesting to multiple paths that corporations can take to achieve social innovation.