bluuebunny/crossref_metadata_2025_split huggingface dataset:
A workflow for streamlined acquisition and correlation of serial regions of interest in array tomography
Authors: Gabarre Sergio , Vernaillen Frank , Baatsen Pieter , Vints Katlijn , Cawthorne Christopher , Boeynaems Steven , Michiels Emiel , Vandael Dorien , Gounko Natalia V. , Munck Sebastian 
Published: July 2021

AbstractBackgroundArray tomography (AT) is a high-resolution imaging method to resolve fine details at the organelle level and has the advantage that it can provide 3D volumes to show the tissue context. AT can be carried out in a correlative way, combing light and electron microscopy (LM, EM) techniques. However, the correlation between modalities can be a challenge and delineating specific regions of interest in consecutive sections can be time-consuming. Integrated light and electron microscopes (iLEMs) offer the possibility to provide well-correlated images and may pose an ideal solution for correlative AT. Here, we report a workflow to automate navigation between regions of interest.ResultsWe use a targeted approach that allows imaging specific tissue features, like organelles, cell processes, and nuclei at different scales to enable fast, directly correlated in situ AT using an integrated light and electron microscope (iLEM-AT). Our workflow is based on the detection of section boundaries on an initial transmitted light acquisition that serves as a reference space to compensate for changes in shape between sections, and we apply a stepwise refinement of localizations as the magnification increases from LM to EM. With minimal user interaction, this enables autonomous and speedy acquisition of regions containing cells and cellular organelles of interest correlated across different magnifications for LM and EM modalities, providing a more efficient way to obtain 3D images. We provide a proof of concept of our approach and the developed software tools using both Golgi neuronal impregnation staining and fluorescently labeled protein condensates in cells.ConclusionsOur method facilitates tracing and reconstructing cellular structures over multiple sections, is targeted at high resolution ILEMs, and can be integrated into existing devices, both commercial and custom-built systems.
Exosome-derived noncoding RNAs in gastric cancer: functions and clinical applications
Authors: Tang Xiao-Huan , Guo Ting , Gao Xiang-Yu , Wu Xiao-Long , Xing Xiao-Fang , Ji Jia-Fu , Li Zi-Yu 
Published: July 2021

AbstractExosomes are a subpopulation of the tumour microenvironment (TME) that transmit various biological molecules to promote intercellular communication. Exosomes are derived from nearly all types of cells and exist in all body fluids. Noncoding RNAs (ncRNAs) are among the most abundant contents in exosomes, and some ncRNAs with biological functions are specifically packaged into exosomes. Recent studies have revealed that exosome-derived ncRNAs play crucial roles in the tumorigenesis, progression and drug resistance of gastric cancer (GC). In addition, regulating the expression levels of exosomal ncRNAs can promote or suppress GC progression. Moreover, the membrane structures of exosomes protect ncRNAs from degradation by enzymes and other chemical substances, significantly increasing the stability of exosomal ncRNAs. Specific hallmarks within exosomes that can be used for exosome identification, and specific contents can be used to determine their origin. Therefore, exosomal ncRNAs are suitable for use as diagnostic and prognostic biomarkers or therapeutic targets. Regulating the biogenesis of exosomes and the expression levels of exosomal ncRNAs may represent a new way to block or eradicate GC. In this review, we summarized the origins and characteristics of exosomes and analysed the association between exosomal ncRNAs and GC development.
Examination of the psychometric properties of the persian version of the COVID-19-impact on Quality of Life Scale
Authors: Dehkordi Ali Hasanpour , Aslani Marzieh , Ebadi Abbas , Repišti Selman , Moradveisi Borhan , Gheshlagh Reza Ghanei 
Published: July 2021

Abstract
Introduction
As a result of high transmission and mortality rates, the Covid-19 pandemic has led to a worldwide health crisis, isolation, and widespread fear, therefore negatively influencing people’s quality of life (QOL). The goal of the present study was to examine the psychometric properties (validity and reliability) of the Persian version of the COVID-19-Impact on Quality of Life (COV19-QoL) scale.

Methods
After translating the scale using the forward–backward method, face and content validly was qualitatively assessed. Then the scale was distributed to 488 individuals from the general population via online platforms. Construct validity was assessed using exploratory (EFA) and confirmatory (CFA) factor analysis. In addition, internal consistency was examined using Cronbach’s alpha coefficient and McDonald’s omega, relative stability was assessed using interclass correlation coefficient (ICC), and absolute stability was calculated through examination of standard error of measurement.

Results
The EFA revealed one factor that explained 55.96% of the total variance of the scale. Internal consistencies of 0.823 and 0.882 were found using Cronbach’s alpha coefficient and McDonald’s omega, respectively. In addition, an ICC of 0.837 (with a two-week interval) was found. Covid-19 had a greater impact on the QOL of healthy participants than that of those with underling conditions (p = 0.004), and also on the QOL of single participants than that of married ones (p = 0.032).

Conclusion
The Persian version of the COV19-QoL is a valid and reliable instrument that can be used to examine the impact of Covid-19 on QOL.
A digital health peri-operative cognitive-behavioral intervention to prevent transition from acute to chronic postsurgical pain in adolescents undergoing spinal fusion (SurgeryPalTM): study protocol for a multisite randomized controlled trial
Authors: Rabbitts Jennifer A. , Zhou Chuan , de la Vega Rocio , Aalfs Homer , Murray Caitlin B. , Palermo Tonya M. 
Published: July 2021

Abstract
Background
Spinal fusion surgery is associated with severe acute postsurgical pain and high rates of chronic postsurgical pain in adolescents. Psychological distress, sleep disturbance, and low pain self-efficacy predict higher acute pain and likelihood of developing chronic postsurgical pain. Interventions targeting baseline psychosocial risk factors have potential to interrupt a negative trajectory of continued pain and poor health-related quality of life (HRQL) over time but have not yet been developed and evaluated. This randomized controlled trial will test effectiveness of a digital peri-operative cognitive-behavioral intervention (SurgeryPalTM) vs. education-control delivered to adolescents and their parents to improve acute and chronic pain and health outcomes in adolescents undergoing spine surgery.

Methods
Adolescents 12–18 years of age undergoing spinal fusion for idiopathic conditions, and their parent, will be recruited from pediatric centers across the USA, for a target complete sample of 400 dyads. Adolescents will be randomized into 4 study arms using a factorial design to SurgeryPalTM or education control during 2 phases of treatment: (1) pre-operative phase (one-month before surgery) and (2) post-operative phase (1 month after surgery). Acute pain severity and interference (primary acute outcomes) and opioid use will be assessed daily for 14 days following hospital discharge. Chronic pain severity and interference (primary acute outcomes), as well as HRQL, parent and adolescent distress, sleep quality, and opioid use/misuse (secondary outcomes), will be assessed at 3 months and 6 months post-surgery.

Discussion
Demonstration of effectiveness and understanding optimal timing of perioperative intervention will enable implementation of this scalable psychosocial intervention into perioperative care. Ultimately, the goal is to improve pain outcomes and reduce reliance on opioids in adolescents after spine surgery.

Trial registration
NCT04637802 ClinicalTrials.gov. Registered on November 20, 2020
Bioengineering of LAB vector expressing Haemolysin co-regulated protein (Hcp): a strategic approach to control gut colonization of Campylobacter jejuni in a murine model
Authors: Gorain Chandan , Khan Afruja , Singh Ankita , Mondal Samiran , Mallick Amirul Islam 
Published: July 2021

Abstract
Background
Campylobacter jejuni (C. jejuni) is accountable for more than 400 million cases of gastroenteritis each year and is listed as a high-priority gut pathogen by the World Health Organization (WHO). Although the acute infection of C. jejuni (campylobacteriosis) is commonly treated with macrolides and fluoroquinolones, the emergence of antibiotic resistance among C. jejuni warrants the need for an alternative approach to control campylobacteriosis in humans. To this end, vaccines remain a safe, effective, and widely accepted strategy for controlling emerging and re-emerging infectious diseases. In search of a suitable vaccine against campylobacteriosis, recently, we demonstrated the potential of recombinant Haemolysin co-regulated protein (Hcp) of C. jejuni Type VI secretion system (T6SS) in imparting significant immune-protection against cecal colonization of C. jejuni; however, in the avian model. Since clinical features of human campylobacteriosis are more complicated than the avians, we explored the potential of Hcp as a T6SS targeted vaccine in a murine model as a more reliable and reproducible experimental host to study vaccine-induced immune-protection against C. jejuni. Because C. jejuni primarily utilizes the mucosal route for host pathogenesis, we analyzed the immunogenicity of a mucosally deliverable bioengineered Lactic acid bacteria (LAB), Lactococcus lactis (L. lactis), expressing Hcp. Considering the role of Hcp in both structural (membrane-bound) and functional (effector protein) exhibition of C. jejuni T6SS, a head-to-head comparison of two different forms of recombinant LAB vectors (cell wall anchored and secreted form of Hcp) were tested and assessed for the immune phenotypes of each modality in BALB/c mice.

Results
We show that regardless of the Hcp protein localization, mucosal delivery of bioengineered LAB vector expressing Hcp induced high-level production of antigen-specific neutralizing antibody (sIgA) in the gut with the potential to reduce the cecal load of C. jejuni in mice.

Conclusion
Together with the non-commensal nature of L. lactis, short gut transit time in humans, and the ability to express the heterologous protein in the gut, the present study highlights the benefits of bioengineered LAB vectors based mucosal vaccine modality against C. jejuni without the risk of immunotolerance.
Retroperitoneal pneumatosis and subcutaneous emphysema after oblique lateral interbody fusion surgery: a case report
Authors: Liu Chen , Zhang Yu , Ge Xin , Xiao Liang , Xu Hongguang 
Published: July 2021

Abstract
Background
The oblique lateral interbody fusion technique has received increasing attention for the treatment of degenerative disc disease in recent years. A series of complications have been reported, such as vascular injury, sympathetic chain injury, and transient psoas weakness, although it is regarded as a relatively safe technique.

Case presentation
A 55-year-old male patient of Han nationality was diagnosed with lumbar spinal stenosis and underwent standalone oblique lateral interbody fusion surgery under general anethesia. Three days after the operation, subcutaneous gas accumulation appeared in the left lower abdomen of the patient with no inflammatory reaction of the wound. He was treated with conservative management, and the retroperitoneal pneumatosis and subcutaneous emphysema had disappeared completely 1 month later.

Conclusions
To date, this is the first reported case of retroperitoneal pneumatosis and subcutaneous emphysema related to oblique lateral interbody fusion surgery, which broadens the scope of the complications of oblique lateral interbody fusion surgery.
High performance decoupled active and reactive power control for three-phase grid-tied inverters using model predictive control
Authors: Azab Mohamed 
Published: July 2021

AbstractFinite control set-model predictive control (FCS-MPC) is employed in this paper to control the operation of a three-phase grid-connected string inverter based on a direct PQ control scheme. The main objective is to achieve high-performance decoupled control of the active and reactive powers injected to the grid from distributed energy resources (DER).The FCS-MPC scheme instantaneously searches for and applies the optimum inverter switching state that can achieve certain goals, such as minimum deviation between reference and actual power; so that both power components (P and Q) are well controlled to their reference values.In addition, an effective method to attenuate undesired cross coupling between the P and Q control loops, which occurs only during transient operation, is investigated. The proposed method is based on the variation of the weight factors of the terms of the FCS-MPC cost function, so a higher weight factor is assigned to the cost function term that is exposed to greater disturbance. Empirical formulae of optimum weight factors as functions of the reference active and reactive power signals are proposed and mathematically derived. The investigated FCS-MPC control scheme is incorporated with the LVRT function to support the grid voltage in fulfilling and accomplishing the up-to-date grid codes. The LVRT algorithm is based on a modification of the references of active and reactive powers as functions of the instantaneous grid voltage such that suitable values of P and Q are injected to the grid during voltage sag.The performance of the elaborated FCS-MPC PQ scheme is studied under various operating scenarios, including steady-state and transient conditions. Results demonstrate the validity and effectiveness of the proposed scheme with regard to the achievement of high-performance operation and quick response of grid-tied inverters during normal and fault modes.
Prevalence of esophageal eosinophilia in patients referred for diagnostic upper gastrointestinal endoscopy
Authors: Ahmed Nancy Abdel Fattah , Amer Hebat-Allah Moheb , Ibrahim Dina Abdallah , El-Zayyadi Islam Abd El-Hamid 
Published: July 2021

Abstract
Background
Several conditions are associated with esophageal eosinophilia such as eosinophilic esophagitis (EoE) and gastro-esophageal reflux disease (GERD). The aim of this study was to detect the prevalence of esophageal eosinophilia in patients with upper gastrointestinal (GI) symptoms referred for diagnostic upper GI endoscopy. This study included 86 patients who underwent upper GI endoscopy and biopsies.

Results
Esophageal eosinophilia EE was found in 26 patients (30.2%): 3 patients (3.5%) had EoE and 23 patients (26.7%) had low-grade esophageal eosinophilia. The most common presenting symptoms were heart burn in 84 patients (97.7%) and upper abdominal pain in 78 patients (90.7%). Reflux esophagitis (ERD) was observed in 18.6% of patients. In histopathological examination, EoE was found in 3.5%, mild reflux esophagitis in 37.2%, and severe reflux esophagitis in 16.3%. There is statistically significant correlation between EE and male sex, hypertension, dysphagia, hiatus hernia, incompetent cardia, and fixed rings. Age, incompetent cardia, and dysphagia were statistically significant independent predictors of low-grade EE.

Conclusion
Esophageal eosinophilia EE was found in 30.2% of patients: 3.5% had eosinophilic esophagitis EoE and 26.7% had low-grade esophageal eosinophilia.
Televised Tears
Authors: Moll Yasmin 
Published: July 2021

AbstractThe practice of feigning weeping in devotional contexts, including in hortatory preaching, is closely associated in Egypt with Islamic Revivalism. It is an expression of pious humility through which worshippers pretend to cry in order to (ideally) develop the embodied capacity to shed tears in the future. Secular Egyptians tend to dismiss such weeping as insincere, but so, too, do many participants in the piety movement in a specific context: on-camera weeping. Drawing on fieldwork with Islamic television preachers and their followers in Cairo, this article explores how the mass-mediated artifice of preacherly weeping provokes expressions of religious ambivalence about an otherwise authoritative ritual practice. While televised tears facilitate the sense of intimacy that many viewers identify as key to their religious adherence, the preacher's lachrymose passion as dramaturgical enskillment coexists uneasily with the pious discipline of sincere self-cultivation. Understanding why this is so illuminates the changing criteria for ritual aptness in a mass media age.
“I Do Not Forgive!”
Authors: Miller Alyssa 
Published: July 2021

Abstract
Reconciliation is a central goal of transitional justice. Yet, its importance for democratization can give reconciliation a coercive edge, pressuring victims to abandon legitimate grievances for the good of the nation to come. This article considers struggles over popular sovereignty in Tunisia's democratic transition, by examining the anticorruption campaign Manish Msamah (“I do not forgive”). Manish Msamah was formed in 2015 to defeat the Project Law on Economic and Financial Reconciliation, legislation that proposed amnesty for crony capitalists who profited from the Ben Ali dictatorship. Drawing on participant observation, media analysis, and activist interviews, the author shows how Manish Msamah debunks the ruse of consent at the heart of reconciliation, and in doing so maintains fidelity to the ideals of the 2011 Revolution. The campaign is revealed as an early participant in the “second wave” of the Arab Spring, which has refused the lure of procedural democracy in favor of deeper structural change.
Juridical Pan-Islam at the Height of Empire
Authors: Yahaya Nurfadzilah 
Published: July 2021

Abstract
Located at the intersection of four regions, the Middle East, East Asia, Central Asia, and South Asia, Afghanistan is a country whose legal history is sure to be diverse and exciting at the confluence of multiple legal currents. In the book Afghanistan Rising: Islamic Law and Statecraft between the Ottoman and British Empires, Faiz Ahmed shows how Afghanistan could be regarded as a pivot for Islamic intellectual currents from the late nineteenth century onward, especially between the Ottoman Empire and South Asia. Afghanistan Rising makes us aware of our own assumptions of the study of Islamic law that has been artificially carved out during the rise of area studies, including Islamic studies. Ahmed provides a good paradigm for a legal history of a country that was attentive to foreign influences without being overwhelmed by them. While pan-Islamism is often portrayed as a defensive ideology that developed in the closing decades of the nineteenth century in reaction to high colonialism, the plotting of Afghanistan's juridical Pan-Islam in Ahmed's book is a robust and powerful maneuver out of this well-trodden path, as the country escaped being “landlocked” mainly by cultivating regional connections in law.
Islamic Modernism, the HanafiMazhab, and Codification in Aman Allah's Afghanistan
Authors: O'Sullivan Michael 
Published: July 2021

AbstractFew works in recent years have enriched the study of Islamic law quite like Faiz Ahmed's Afghanistan Rising: Islamic Law and Statecraft between the Ottoman and British Empires. The book presents an opportunity to interrogate prevailing historiographical debates about the “codification” of Islamic law (as opposed to its “compilation”), and to account for processes of divergence in Islamic legal culture across Eurasia. This response explores some of the prevailing tensions among Ottoman, Afghan, and Indian experts in early twentieth-century Afghanistan. These stemmed from the dissimilar legal training acquired by the actors and the varying character of Islamic modernism in each geographical context. A focus on diverse intellectual trajectories and competing visions of Islamic law furnishes a useful means for accounting for the aporia endemic to Aman Allah's modernizing project.
From whole organism to ultrastructure: progress in axonal imaging for decoding circuit development
Authors: Weaver Cory J. , Poulain Fabienne E. 
Published: July 2021

ABSTRACT
Since the pioneering work of Ramón y Cajal, scientists have sought to unravel the complexities of axon development underlying neural circuit formation. Micrometer-scale axonal growth cones navigate to targets that are often centimeters away. To reach their targets, growth cones react to dynamic environmental cues that change in the order of seconds to days. Proper axon growth and guidance are essential to circuit formation, and progress in imaging has been integral to studying these processes. In particular, advances in high- and super-resolution microscopy provide the spatial and temporal resolution required for studying developing axons. In this Review, we describe how improved microscopy has revolutionized our understanding of axonal development. We discuss how novel technologies, specifically light-sheet and super-resolution microscopy, led to new discoveries at the cellular scale by imaging axon outgrowth and circuit wiring with extreme precision. We next examine how advanced microscopy broadened our understanding of the subcellular dynamics driving axon growth and guidance. We finally assess the current challenges that the field of axonal biology still faces for imaging axons, and examine how future technology could meet these needs.
Duplicated antagonistic EPF peptides optimize grass stomatal initiation
Authors: Jangra Raman , Brunetti Sabrina C. , Wang Xutong , Kaushik Pooja , Gulick Patrick J. , Foroud Nora A. , Wang Shucai , Lee Jin Suk 
Published: July 2021

ABSTRACT
Peptide signaling has emerged as a key component of plant growth and development, including stomatal patterning, which is crucial for plant productivity and survival. Although exciting progress has been made in understanding EPIDERMAL PATTERNING FACTOR (EPF) signaling in Arabidopsis, the mechanisms by which EPF peptides control different stomatal patterns and morphologies in grasses are poorly understood. Here, by examining expression patterns, overexpression transgenics and cross-species complementation, the antagonistic stomatal ligands orthologous to Arabidopsis AtEPF2 and AtSTOMAGEN/AtEPFL9 peptides were identified in Triticum aestivum (wheat) and the grass model organism Brachypodium distachyon. Application of bioactive BdEPF2 peptides inhibited stomatal initiation, but not the progression or differentiation of stomatal precursors in Brachypodium. Additionally, the inhibitory roles of these EPF peptides during grass stomatal development were suppressed by the contrasting positive action of the BdSTOMAGEN peptide in a dose-dependent manner. These results not only demonstrate how conserved EPF peptides that control different stomatal patterns exist in nature, but also suggest new strategies to improve crop yield through the use of plant-derived antagonistic peptides that optimize stomatal density on the plant epidermis.