bluuebunny/crossref_metadata_2025_split huggingface dataset:
Alterations in Sphingolipid Composition and Mitochondrial Complex I Deficiency Represent Synergistic Therapeutic Vulnerabilities Linked to Vincristine Resistance
Authors: Fisher-Wellman Kelsey H. , Hagen James T , Kassai Miki , Kao Li-Pin , Nelson Margaret , McLaughlin Kelsey , Coalson Hannah , Fox Todd , Tan Su-Fern , Feith David , Kester Mark , Loughran Thomas , Claxton David , Cabot Myles 
Published: May 2021

Abstract
Background: Modifications in sphingolipid (SL) metabolism and mitochondrial bioenergetics are key factors implicated in cancer cell response to chemotherapy, including chemotherapy resistance. Vinca alkaloids such as vincristine (VCR), widely used in cancer treatment, are no exception, as their beneficial actions are often supplanted by resistance. In the present work we utilized HL-60 human leukemia cells and a VCR-resistant counterpart, HL-60/VCR cells, to determine potential interplay between SL metabolism and mitochondrial bioenergetics supportive of VCR resistance. Methods: Cellular SL alterations were assessed via a combination of enzyme immunoblot analysis and lipidomics. Mitochondrial function was evaluated in intact cells, permeabilized cells and isolated mitochondria using a comprehensive diagnostic assay system designed to directly interrogate oxidative phosphorylation (OXPHOS) kinetics. Informed by SL and bioenergetic readouts, experiments were designed to assess the therapeutic efficacy of co-targeting SL metabolism and mitochondrial flux to combat VCR resistance. Results: Relative to wild-type cells, HL-60/VCR presented with global alterations in SL composition, typified by upregulated expression of sphingosine kinase (SPHK1), which catalyzes formation of sphingosine 1-phosphate (S1P), glucosylceramide synthase (GCS), which catalyzes formation of glucosylceramides (GC), and acid ceramidase, responsible for ceramide hydrolysis. In support of these changes, VCR resistance was also characterized by increases in S1P, several molecular species of ceramide and GC, and changes in sphingomyelin (SM) molecular species. With respect to mitochondria, despite increased basal respiration in HL-60/VCR cells, direct interrogation of the mitochondrial network revealed intrinsic respiratory complex insufficiency, largely localized to complex I (CI). Importantly, forced ceramide accumulation in wild-type cells phenocopied the respiratory insufficiency observed in HL-60/VCR, and co-targeting SL metabolism and CI induced synergistic cytotoxicity in HL-60/VCR. Conclusions: Together, these data underscore the intimate connection between cellular sphingolipids and mitochondrial metabolism and suggest that pharmacological intervention across both pathways may represent a novel treatment strategy against VCR resistance.
Development of Metal-Based Drugs and Application in Clinical Treatment
Authors: Shao Yi Ming , Sharavyn Bold , Huang Ping , Naranmandura Hua , Wang Qian Qian 
Published: May 2021

Abstract:
Metals occur naturally in soil and many kinds of rocks, particularly in minerals and ores,
which also play a vital role in living systems such as plants and animals. Over the large time scale,
metal evolution from toxins to drugs has achieved a milestone mean in medicine. Currently, a few
metal-based drugs (i.e., metallodrugs) have been used in the clinic to treat patients with different
medical conditions, making exciting new developments in anticancer therapeutics (e.g., arsenic trioxide
and cisplatin) that quickly move into focus. Moreover, a thorough understanding of the properties
and effects of metals on the human body could improve the development and innovation of
metal-based drugs. In this review, we have comprehensively described the discovery and development
of metal-based anticancer drugs, as well as their side effects in clinical treatment. Although
metallodrugs have shown promising outcomes in the treatment of cancers, further investigation is
needed to optimize their side effect for broader applications.
Surgical management and outcome of dogs with primary spontaneous pneumothorax: 110 cases (2009–2019)
Authors: Dickson Rachel , Scharf Valery F. , Michael Aleisha E. , Walker Meagan , Thomson Chris , Grimes Janet , Singh Ameet , Oblak Michelle , Brisson Brigitte , Case J. Brad 
Published: May 2021

OBJECTIVE
To describe surgical management and associated outcomes for dogs with primary spontaneous pneumothorax.

ANIMALS
110 client-owned dogs with primary spontaneous pneumothorax that underwent surgical management.

PROCEDURES
Medical records at 7 veterinary teaching hospitals were reviewed. Data collected included signalment, history, clinical signs, radiographic and CT findings, surgical methods, intraoperative and postoperative complications, outcomes, and histopathologic findings. Follow-up information was obtained by contacting the referring veterinarian or owner.

RESULTS
110 dogs were included, with a median follow-up time of 508 days (range, 3 to 2,377 days). Ninety-nine (90%) dogs underwent median sternotomy, 9 (8%) underwent intercostal thoracotomy, and 2 (2%) underwent thoracoscopy as the sole intervention. Bullous lesions were most commonly found in the left cranial lung lobe (51/156 [33%] lesions) and right cranial lung lobe (37/156 [24%] lesions). Of the 100 dogs followed up for > 30 days, 13 (13%) had a recurrence of pneumothorax, with median time between surgery and recurrence of 9 days. Recurrence was significantly more likely to occur ≤ 30 days after surgery, compared with > 30 days after surgery. Recurrence > 30 days after surgery was rare (3 [3%]). No risk factors for recurrence were identified.

CONCLUSIONS AND CLINICAL RELEVANCE
Lung lobectomy via median sternotomy resulted in resolution of pneumothorax in most dogs with primary spontaneous pneumothorax. Recurrence of pneumothorax was most common in the immediate postoperative period, which may have reflected failure to identify lesions during the initial thoracic exploration, rather than development of additional bullae.
Study of the solubilisation process of bacterial model membranes induced by DDAO
Authors: Želinská K. , Gallová J. 
Published: May 2021

Abstract
Solubilisation of two bacterial model membranes induced by N,N-dimethyl-1-dodecanamine-N-oxide (DDAO) was studied. The first model membrane consisted of a mixture of palmitoyloleoylphosphatidylethanolamine (POPE) and palmitoyloleoylphosphatidylglycerol (POPG) in a molar ratio 0.6:0.4 mol/mol, and a second model membrane was enriched with tetraoleoylcardiolipin (TOCL) with a composition POPE-POPG-TOCL = 0.67:0.23:0.1 mol/mol/mol. Solubilisation of these model membranes was studied by static light scattering (nephelometry). Effective ratio Re (the amount of DDAO integrated into the bilayer to the amount of lipid) at different steps of the solubilisation process was determined. The molar partition coefficient of DDAO was calculated – in case of the POPE-POPG membrane, Kp = 5,300 ± 400, for the POPE-POPG-TOCL membrane, Kp = 6,500 ± 500.
Eco-Fashion Designing to Ensure Corporate Social Responsibility within the Supply Chain in Fashion Industry
Authors: Jutidamrongphan Warangkana , Rahman Md Ahbabur , Hossain Tareq , Khatun Syeda Abida , Lamas Wendell de Queiróz 
Published: May 2021

Abstract
Fashion is a widespread cultural phenomenon which supports cultural attitudes and proposes new ways of acting. This study considers how ecological goals can be applied in the fashion industry through an awareness of its effects on the environment with the aim of conducting the process of designing and manufacturing fashion garments. To understand this needs practically, this study investigates on few industries in Hatyai municipality (Thailand) for knowing their practice on eco-fashion approach to support MSW management properly. The study adopts as its methodology a systematic review of relevant literature covering the twin problems of the degradation of the environment and the shortage of natural resources currently caused by the fashion-garment industry, in search of feasible solutions centred on the concept of eco-fashion. The study also addresses how the concept of ecological clothing can generate a new mentality among consumers. The study identifies concepts, techniques and processes which can economically be applied in producing fashion garments from a more ecologically sound starting point. This paper also sheds light on how corporate social responsibility (CSR) can be practiced within the fashion supply chain. This study contributes to the existing literature by assisting the understanding of the current situation of waste in the fashion-garment industry and the methods necessary to ensure eco-fashion designing and a responsible fashion supply chain.
Renal Recovery in Critically Ill Adult Patients Treated With Veno-Venous or Veno-Arterial Extra Corporeal Membrane Oxygenation: A Retrospective Cohort Analysis
Authors: Thyagarajan Braghadheeswar , Murea Mariana , Jones Deanna N. , Saha Amit K. , Russell Gregory B. , Khanna Ashish K. 
Published: May 2021

Abstract

Introduction
Patients on extracorporeal membrane oxygenator (ECMO) therapy are critically ill and often develop acute kidney injury (AKI) during hospitalisation. Little is known about the association of exposure to and the effect of the type of ECMO and extent of renal recovery after AKI development.

Aim of the study
In patients who developed AKI, renal recovery was characterised as complete, partial or dialysis-dependent at the time of hospital discharge in both the Veno-Arterial (VA) and Veno-Venous (VV) ECMO treatment groups.

Material and methods
The study consisted of a single-centre retrospective cohort that includes all adult patients (n=125) who received ECMO treatment at a tertiary academic medical centre between 2015 to 2019. Data on demographics, type of ECMO circuit, comorbidities, exposure to nephrotoxic factors and receipt of renal replacement therapy (RRT) were collected as a part of the analysis. Acute Kidney Injury Network (AKIN) criteria were used for the diagnosis and classification of AKI. Group differences were assessed using Fisher’s exact tests for categorical data and independent t-tests for continuous outcomes.

Results
Sixty-four patients received VA ECMO, and 58 received VV ECMO. AKI developed in 58(91%) in the VA ECMO group and 51 (88%) in the VV ECMO group (p=0.77). RRT was prescribed in significantly higher numbers in the VV group 38 (75%) compared to the VA group 27 (47%) (p=0.0035). At the time of discharge, AKI recovery rate in the VA group consisted of 15 (26%) complete recovery and 5 (9%) partial recovery; 1 (2%) remained dialysis-dependent. In the VV group, 22 (43%) had complete recovery (p=0.07), 3(6%) had partial recovery (p=0.72), and 1 (2%) was dialysis-dependent (p>0.99). In-hospital mortality was 64% in the VA group and 49% in the VV group (p=0.13).

Conclusions
Renal outcomes in critically ill patients who develop AKI are not associated with the type of ECMO used. This serves as preliminary data for future studies in the area.
Prevalence of Depression, Anxiety and Stress among Patients Discharged from Critical Care Units
Authors: Saeidi Mohammad , Safaei Alireza , Sadat Zohreh , Abbasi Parisa , Sarcheshmeh Masoumeh Sadat Mousavi , Dehghani Fariba , Tahrekhani Mehran , Abdi Mohammad 
Published: May 2021

Abstract

Introduction
The widespread use of advanced technology and invasive intervention creates many psychological problems for hospitalized patients; it is especially common in critical care units.

Methods
This cross-sectional study was conducted on 310 patients hospitalized in critical care units, using a non-probability sampling method. Data were collected using depression, anxiety, and stress scale (DASS-21) one month after discharge from the hospital. Data analysis was performed using descriptive and inferential statistics.

Results
181 males and 129 females with a mean age (SD) of 55.11(1.62) years were enrolled in the study. The prevalence of depression, anxiety and stress were 46.5, 53.6 and 57.8% respectively, and the depression, anxiety and stress mean (SD) scores were 16.15(1.40), 18.57(1.46), 19.69(1.48), respectively. A statistically significant association was reported between depression, anxiety and stress with an increase in age, the number of children, occupation, education, length of hospital stay, use of mechanical ventilation, type of the critical care unit, and drug abuse.

Conclusion
The prevalence of depression, anxiety and stress in patients discharged from critical care units was high. Therefore, crucial decisions should be made to reduce depression, anxiety and stress in patients discharged from critical care units by educational strategies, identifying vulnerable patients and their preparation before invasive diagnostic-treatment procedures.
Neumomediastino espontáneo: ¿una patología benigna?
Authors: Baeza Carrión Ana , Cornejo López María de los Ángeles , Garrido Benito Beatriz , Pascual Nadal Juan José , Velayos García Paula , Ripoll Martín Roberto , Ots Gutiérrez José Ramón , Oliver García Israel 
Published: May 2021

Introducción y objetivos: El neumomediastino espontáneo se define como aire intersticial en el mediastino sin causa primaria establecida que predomina en varones jóvenes adultos. El objetivo de este estudio es informar sobre el manejo habitual en nuestro hospital y proponer un algoritmo diagnóstico-terapéutico.
Materiales y métodos: Estudio descriptivo y retrospectivo de 25 casos de neumomediastino espontáneo diagnosticados en nuestro hospital, en un período de 25 años.
Se analizaron datos demográficos, factores predisponentes y desencadenantes, clínica, diagnóstico, tratamiento y recurrencias.
Resultados: La edad media fue de 22 años, con asma, inhalación de drogas, extracciones dentarias o tabaquismo como factores predisponentes. Los síntomas más frecuentes fueron dolor torácico, disnea, disfonía y disfagia/odinofagia. El diagnóstico inicial se obtuvo mediante radiografía de tórax, completándose en ocasiones con tomografía computarizada, esófagograma o laringoscopía. Todos los pacientes ingresaron manejándose de forma conservadora. Durante el seguimiento el 12 % presentó recurrencia del neumomediastino, resolviéndose sin complicaciones.
Discusión: La presencia de aire libre mediastínica puede percibirse como hallazgo de mal pronóstico, pero estudios previos concluyen que el neumomediastino espontáneo cuenta con un curso benigno, aunque el diagnóstico debe hacerse por exclusión, pues el grosso de la evidencia lo constituye el neumomediastino secundario.
Conclusiones:El neumomediastino espontáneo es una entidad benigna e infrecuente, que puede tener factores predisponentes y desencadenantes, si bien no existe causa primaria aparente. Se resuelve habitualmente de forma conservadora en pocos días, aunque pueden existir recurrencias.
Justification of the use of a vegetal additive to diesel fuel as a method of protecting underground personnel of coal mines from the impact of harmful emissions of diesel-hydraulic locomotives
Authors: Korshunov G. , Eremeeva Anzhelika , Drebenstedt Carsten 
Published: May 2021

Equipment with diesel engines is used in all mining enterprises. Monorail diesel transport is of great importance in coal mines, as it facilitates the heavy labor of workers when transporting materials and people, fixing mining workings, refueling and repairing equipment, which leads to an increase in the speed of tunneling operations. Reducing the concentration of harmful gases from diesel-hydraulic locomotives at the workplaces of coal mine locomotive drivers can be ensured by the use of additives to diesel fuel that reduce the volume of harmful gas emissions during the operation of diesel-hydraulic locomotives. Additive ester-based on vegetal oil in the amount of 5 mass % in a mixture with hydrotreated diesel fuel reduces the concentration of carbon monoxide by 19-60 %, nitrogen oxides by 17-98 %, depending on the operating mode of the engine, the smoke content of the exhaust gases is reduced to 71 %. There is an improvement in working conditions at the workplace of the driver of a diesel-hydraulic locomotive by the chemical factor due to the reduction of the class of working conditions from 3.1. to 2.
Repüléskoordináló és -optimalizáló rendszer
Authors: Békési Bertold , Sári János , Gajdács László 
Published: May 2021

A cikkben a szerzők bemutatják a repüléskoordináló és - optimalizáló rendszert. Rövid bevezetést követően bemutatjuk az FMS funkcióit, felépítését. Ismertetjük a rendszer elemeit, típusait, valamint azok alapvető működését is. Célunk egy átfogó, könnyen megérthető összefoglaló elkészítése a repüléskoordináló és - optimalizáló rendszerről.
Performance and Health Decrements Associated With Relative Energy Deficiency in Sport for Division I Women Athletes During a Collegiate Cross-Country Season: A Case Series
Authors: Hooper David R. , Mallard Jared , Wight Jeff T. , Conway Kara L. , Pujalte George G.A. , Pontius Kelsey M. , Saenz Catherine , Hackney Anthony C. , Tenforde Adam S. , Ackerman Kathryn E. 
Published: May 2021

The purpose of this case series was to evaluate the presence of low Energy Availability (EA) and its impact on components of Relative Energy Deficiency in Sport (RED-S) in a population of female collegiate runners. Seven female NCAA Division I athletes (age: 22.3 ± 1.5 yrs; height: 169.7 ± 5.7 cm; weight: 58.3 ± 4.1 kg) were tracked from August until February, covering the beginning (Pre XC), end (Post XC) of their competitive cross country season, and beginning of the following track season (Pre Track). The athletes were assessed for female athlete triad (Triad) risk, energy availability, body composition, resting metabolic rate (RMR), nutritional intake, and blood markers (including vitamin D, ferritin, and triiodothyronine (T3)). From Pre XC to Post XC there were no significant differences in body mass, fat free mass or body fat percentage. At Pre XC, mean EA was 31.6 ± 13.3 kcal/kg FFM∙d-1. From Post XC to Pre Track, there was a significant increase in body mass (59.1 ± 5.1 to 60.6 ± 5.7 kg, p&lt;0.001,d=0.27). From Post XC to Pre Track, there was a significant increase in RMR (1466 ± 123.6 to 1614.6 ± 89.1 kcal·d-1, p&lt;0.001,d=2.6). For 25(OH) vitamin D, there was a significant reduction from Pre XC to Post XC (44.1 ± 10.6 vs 39.5 ± 12.2 ng·mL-1, p=0.047,d=-0.4), and a significant increase from Post XC to Pre Track (39.5 ± 12.2 vs. 48.1 ± 10.4 ng·mL-1, p=0.014,d=0.75). For ferritin, there was a trend towards a decrease from Pre XC to Post XC (24.2 ± 13.2 vs. 15.7 ± 8.8 ng·mL-1, p=0.07, d=-0.75), as well as a trend toward an increase from Post XC to Pre Track (15.7 ± 8.8 vs. 34.1 ± 18.0 ng·mL-1, p=0.08, d=1.3). No differences in T3 were observed across time points. Average Triad risk score was 2.3 ± 1.4. Notably, 5 of 7 athletes met criteria for moderate risk. Despite many athletes meeting criteria for low EA and having elevated Triad risk assessment scores, most were able to maintain body mass and RMR. One athlete suffered severe performance decline and a reduced RMR. Surprisingly, she was the only athlete above the recommended value for ferritin. Following increased nutritional intake and reduced training volume, her performance and RMR recovered. Changes in body mass and body composition were not indicative of the presence of other concerns associated with RED-S. This exploratory work serves as a guide for future, larger studies for tracking athletes, using RMR and nutritional biomarkers to assess RED-S.
Autoantibodies Against Ubiquitous and Confined Antigens in Patients With Ocular, Neuro-Ophthalmic and Congenital Cerebral Toxoplasmosis
Authors: Goldberg-Murow Monica , Cedillo-Peláez Carlos , Concha-del-Río Luz Elena , Cheja-Kalb Rashel , Salgar-Henao María José , Orozco-Velasco Eduardo , Luna-Pastén Héctor , Gómez-Chávez Fernando , Ibarra Antonio , Correa Dolores 
Published: May 2021

Toxoplasma gondii infection can trigger autoreactivity by different mechanisms. In the case of ocular toxoplasmosis, disruption of the blood-retinal barrier may cause exposure of confined retinal antigens such as recoverin. Besides, cross-reactivity can be induced by molecular mimicry of parasite antigens like HSP70, which shares 76% identity with the human ortholog. Autoreactivity can be a determining factor of clinical manifestations in the eye and in the central nervous system. We performed a prospective observational study to determine the presence of autoantibodies against recoverin and HSP70 by indirect ELISA in the serum of 65 patients with ocular, neuro-ophthalmic and congenital cerebral toxoplasmosis. We found systemic autoantibodies against recoverin and HSP70 in 33.8% and 15.6% of individuals, respectively. The presence of autoantibodies in cases of OT may be related to the severity of clinical manifestations, while in cases with CNS involvement they may have a protective role. Unexpectedly, anti-recoverin antibodies were found in patients with cerebral involvement, without ocular toxoplasmosis; therefore, we analyzed and proved cross-reactivity between recoverin and a brain antigen, hippocalcin, so the immunological phenomenon occurring in one immune-privileged organ (e.g. the central nervous system) could affect the environment of another (egg. the eye).
Clinical Aspects of Stevens-Johnson Syndrome/Toxic Epidermal Necrolysis With Severe Ocular Complications in Taiwan
Authors: Ma David Hui-Kang , Tsai Tsung-Ying , Pan Li-Yen , Chen Shin-Yi , Hsiao Ching-Hsi , Yeh Lung-Kun , Tan Hsin-Yuan , Lu Chun-Wei , Chen Chun-Bing , Chung Wen-Hung 
Published: May 2021

Purpose: Over the last decade, there has been tremendous progress in the treatment of Stevens-Johnson syndrome (SJS) and toxic epidermal necrolysis (TEN). To understand whether this has resulted in better ophthalmic outcomes, we aimed to study the incidence of severe ocular complications (SOCs) in the acute and chronic stage among SJS/TEN patients, major causative medications, and therapeutic effect of medical and surgical treatment.Methods: Using electronic medical records review of patients of Chang Gung Memorial Hospital Linkou Branch from 2010 to 2020, 119 patients (236 eyes) received ophthalmic consultation during the acute stage and were retrospectively studied. Sotozono's grading score systems for acute and chronic SJS/TEN were employed for accessing correlation between acute and chronic presentations, the therapeutic effect of systemic etanercept treatment, and outcome of early amniotic membrane transplantation (AMT) performed in patients with severe acute SOCs.Results: There were 46 male and 73 female patients with a mean age of 45.6 ± 22.7 years old (2–90 years), and follow-up time of 408.3 ± 351.0 (116–1,336) days. The numbers of patients with SJS, overlap syndrome, and TEN were 87, 9, and 23, respectively. In total, 109 eyes (55 patients) had acute SOCs, which comprised 46.2% of patients who underwent ophthalmic examination. Antiepileptics were the most common category of culprit drugs causing SOCs in the acute stage. At the end of follow-up, there were 14 eyes (9 patients) with chronic SOCs (5.9%), and non-steroidal anti-inflammatory drugs and cold medicine were the most common causative medications that were associated with severe chronic sequela. The correlation between Sotozono's acute and chronic grading score showed a positive relationship [Spearman's rank correlation coefficient (r) = 0.52, p &lt; 0.001]. The average chronic grading scores in patients receiving systemic corticosteroid combined with etanercept treatment were significantly lower than those receiving corticosteroid only, Finally, the average chronic grading scores in patients receiving AMT &lt;7 days after onset were significantly lower than those performed beyond 7 days.Conclusion: Our study implies that acute manifestation can be an indicator for chronic sequelae. Additional early etanercept treatment and early AMT showed beneficial effect in reducing chronic ocular sequela.