bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Role of TOP2A Protein Expression and Aneuploidy in NSCLC
Authors: Chen Zhenwen , Zeng Zijuan , Wang Qi , Xi Yanfeng , Xu Yirong , Yang Ning , Chai Fei , Zhao Yingying , Gong Yuanyuan , Qian Siyi , Wen Tingting 
Published: December 2021

Abstract
Purpose

DNA topoisomerase II alpha (TOP2A) is a cell-cycle dependent protein associated with cell proliferation and division. Abnormal regulation of TOP2A and aneuploidy induces tumorigenesis and poor prognosis. Data related to TOP2A protein in non-small cell lung cancer (NSCLC) is limited to few studies on gene status. The present study aimed to investigate the consistency between aneuploidy and expression of TOP2A gene and protein, respectively, and the role of aneuploidy in the prognosis of NSCLC patients.
Methods

Clinical data and lung cancer tissues were collected from 244 patients with NSCLC. TOP2A protein and gene expression were detected using immunohistochemistry (IHC) and fluorescence in situ hybridization (FISH), respectively. Nonparametric Spearman’s rank sum test was used to analyze clinicopathological data. Kaplan–Meier and multivariate Cox regression methods were used for survival analysis.
Results

Enhanced expression and amplification rate of TOP2A protein and gene were 29.9% (73/244) and 0.4% (1/244), respectively. The aneuploidy rate was 31.6% (77/244). In NSCLC, patients with enhanced expression of TOP2A protein and aneuploidy correlated with clinical stages (p < 0.001) Enhanced expression of TOP2A protein was consistent with aneuploidy as detected by Kappa test (K = 0.307) and this correlation was confirmed by chi-square test (p < 0.001). The overall survival of patients with aneuploidy was shorter than diploidy (p < 0.001). Clinically advanced patients with aneuploidy together with TOP2A overexpression had poor prognosis (p < 0.001).
Conclusion

Aneuploidy and overexpression of TOP2A is a predictor of poor prognosis in patients with advanced NSCLC.
Drug Knowledge Extraction Framework with Entity Pair Calibration for Chinese Drug Instructions
Authors: Zhang Xiaoliang , Zhou Lunsheng , Gao Feng , Wang Zhongmin , Wang Yongqing , Guo Jianjun , Jing Shenqi , Miao Shumei , Zhang Xin , Shan Tao , Liu Yun 
Published: December 2021

Abstract
Existing pharmaceutical information extraction research often focus on standalone entity or relationship identification tasks over drug instructions. There is a lack of a holistic solution for drug knowledge extraction. Moreover, current methods perform poorly in extracting fine-grained interaction relations from drug instructions. To solve these problems, this paper proposes an information extraction framework for drug instructions. The framework proposes deep learning models with fine-tuned pre-training models for entity recognition and relation extraction, in addition, it incorporates an novel entity pair calibration process to promote the performance for fine-grained relation extraction. The framework experiments on more than 60k Chinese drug description sentences from 4000 drug instructions. Empirical results show that the framework can successfully identify drug related entities (F1 >= 0.95) and their relations (F1 >= 0.83) from the realistic dataset, and the entity pair calibration plays an important role (~5% F1 score improvement) in extracting fine-grained relations.
IL-17 Exacerbates Experimental Autoimmune Prostatitis via CXCL1/CXCL2-Mediated Neutrophil Infiltration
Authors: Zhang Cheng , Chen Jia , Wang Hui , Chen Jing , Zheng Mei-Juan , Chen Xian-Guo , Zhang Li , Liang Chao-Zhao , Zhan Chang-Sheng 
Published: December 2021

Abstract
Chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS) is a poorly understood disease. Accumulating evidence suggests that autoimmune dysfunction is involved in the development of CP/CPPS. IL-17 is associated with the occurrence and development of several chronic autoimmune inflammatory diseases. However, the molecular mechanisms underlying the role of IL-17 in CP/CPPS remain unclear. Herein, we first confirmed that IL-17 was increased in the prostate tissues of experimental autoimmune prostatitis (EAP) mice. Corresponding to the increase of IL-17 in the prostate of EAP, neutrophil infiltration and the levels of CXCL1 and CXCL2 (CXC chemokine ligands 1 and 2) were also increased. Treatment of EAP mice with IL-17-neutralizing monoclonal antibody (mAb) resulted in a decreased number of infiltrated neutrophils, as well as the CXCL1 and CXCL2 level. Depletion of neutrophil by anti-Ly6G antibodies ameliorated inflammatory changes and hyperalgesia caused by EAP. Fucoidan, which could potently inhibit neutrophil migration, could also ameliorate the manifestations of EAP. Our finding suggested that IL-17 promoted the production of CXCL1 and CXCL2, which subsequently triggered neutrophil chemotaxis to prostate tissues. And fucoidan might be a potential drug for the therapy of EAP by the effectively inhibiting on neutrophil infiltration.
Birefringent elastomers with Multiple Stimuli-Responses
Authors: Gao Yiyang , Sun Danqi , Chen Jing , Shi Lei , Da Xinyu , Guo Haoyu , Xi Kai , Zhang Dongyang , Gao Ting , Gao Guoxin , Lu Tongqing , Ding Shujiang 
Published: December 2021

Abstract
Currently, even though birefringent soft materials have attracted considerable attention for many sensors and optical devices, it is still difficult for most of them to achieve multiple responses. Herein, we have designed a multiple-responding birefringent elastomer with high birefringence but low modulus by using 2-phenoxy-ethanoacrylate (PHEA) as monomer and 4-cyano-4'pentylbiphenyl as solvent. The excellent transparency (~90%) exhibited by the designed birefringent elastomer allowed us to observe the change of interference color. The obtained birefringent elastomer not only can be used as a photoelastic strain sensor with high sensitivity and fast response but also show a very sensitive response to heat, particularly in the range of the human body temperature. More interestingly, it shows excellent dielectric properties with a strong correlation between the interference color and the applied electric field, which allows easily writing and erasing the encrypted information. These unique multi-signal response features of our obtained birefringent elastomer shed light on the multiple information encryptions, the anti-counterfeiting, and the multifunctional sensors.
Evaluation of transplacental transfer of mRNA vaccine products and functional antibodies during pregnancy and early infancy
Authors: Prahl Mary , Golan Yarden , Cassidy Arianna , Matsui Yusuke , Li Lin , Alvarenga Bonny , Chen Hao , Jigmeddagva Unurzul , Lin Christine , Gonzalez Veronica , Chidboy Megan , Warrier Lakshmi , Buarpung Sirirak , Murtha Amy , Flaherman Valerie , Greene Warner , Wu Alan , Lynch Kara , Rajan Jayant , Gaw Stephanie 
Published: December 2021

Abstract
Studies are needed to evaluate the safety and effectiveness of mRNA SARS-CoV-2 vaccination during pregnancy, and the levels of protection provided to their newborns through placental transfer of antibodies. We evaluated the transplacental transfer of mRNA vaccine products and functional anti-SARS-CoV-2 antibodies during pregnancy and early infancy in a cohort of 20 individuals vaccinated during pregnancy. We found no evidence of mRNA vaccine products in maternal blood, placenta tissue, or cord blood at delivery. However, we found time-dependent efficient transfer of IgG and neutralizing antibodies to the neonate that persisted during early infancy. Additionally, using phage immunoprecipitation sequencing, we found a vaccine-specific signature of SARS-CoV-2 Spike protein epitope binding that is transplacentally transferred during pregnancy. In conclusion, products of mRNA vaccines are not transferred to the fetus during pregnancy, however timing of vaccination during pregnancy is critical to ensure transplacental transfer of protective antibodies during early infancy
The impact of lymphadenectomy on lymph node recurrence after performing various treatments for esophageal squamous cell carcinoma
Authors: Shigeno Takashi , Hoshino Akihiro , Matsunaga Shiho , Shimano Rumi , Ishibashi Naoya , Shinohara Hajime , Shiobara Hiroyuki , Tomii Chiharu , Saito Katsumasa , Fujiwara Naoto , Sato Yuya , Kawada Kenro , Tokunaga Masanori , Kinugasa Yusuke 
Published: December 2021

Abstract
BackgroundTreatment for regional lymph node recurrence after initial treatment for esophageal squamous cell carcinoma (ESCC) differs among institutions. Though some retrospective cohort studies have shown that lymphadenectomy for cervical lymph node recurrence is safe and leads to long-term survival, the efficacy remains unclear. In this study, we investigated the long-term outcomes of patients who underwent lymphadenectomy for regional recurrence after treatment for ESCC.Patients and methodsWe retrieved 20 cases in which lymphadenectomy was performed for lymph node recurrence after initial treatment for ESCC in our hospital from January 2003 to December 2016. Initial treatments included esophagectomy, endoscopic resection (ER) and chemoradiotherapy/chemotherapy (CRT/CT). Overall survival (OS) and recurrence-free survival (RFS) after lymphadenectomy were calculated by the Kaplan-Meier method. We also used a univariate analysis with a Cox proportional hazards model to determine factors influencing the long-term outcomes.ResultsThe 5-year OS and RFS of patients who underwent secondary lymphadenectomy for recurrence after initial treatment were 55.0% and 35.3%, respectively. The 5-year overall survival rates of patients who received esophagectomy, ER and CRT/CT as initial treatments, were 45.5%, 80.0% and 50.0%, respectively. The 5-year OS rates of patients with pStage I and pStage II-IVB lymph node recurrence were 75.0% and 41.7%, respectively.ConclusionsLymphadenectomy for regional recurrence after initial treatment for ESCC is effective to some degree. Patients with regional recurrence after initial treatment for Stage I ESCC have a good prognosis; thus, lymphadenectomy should be considered for these cases.
Deep Learning Analysis of Polar Maps from SPECT Myocardial Perfusion Imaging for Prediction of Coronary Artery Disease
Authors: Zahiri Narges , Asgari Rhona , Razavi-Ratki Seid-Kazem , parach Ali-Asghar 
Published: December 2021

Abstract
Purpose: This study aimed to investigate the diagnostic accuracy of deep convolutional neural networks for classifying the polar map images in Single-photon emission computed tomography (SPECT) myocardial perfusion imaging (MPI) by considering the physician’s diagnosis as reference.Methods: 3318 images of stress and rest polar maps related to patients (67% women and 33% men) who underwent 99mTc-sestamibi MPI were collected. The images were manually labeled with normal and abnormal labels according to the doctor’s diagnosis reports. The proposed deep learning model was trained using stress and rest polar maps and evaluated for prediction of obstructive disease in a stratified 5-fold cross-validation procedure.Results: The mean values of accuracy, sensitivity, accuracy, specificity, f1 score, and the area under the roc curve were 0.7562, 0.7856, 0.5748, 0.7434, 0.6646, and, 0.8450, respectively over 5 folds using both stress and rest scans. The inclusion of rest perfusion maps significantly improved AUC of the deep learning model (AUC: 0.845; 95% CI: 0.832-0.857), compared with using stress polar maps only (AUC: 0.827; 95% CI: 0.814-0.840); P < 0.05.Conclusion: The results of the present work reveal the possible applications of deep learning for polar map images classification in SPECT MPI.
Social Determinants of Emergency Department Visits in Mild Compared To Moderate and Severe Asthma
Authors: Alachraf Kamel , Currie Caroline , Wooten William , Tumin Dmitry 
Published: December 2021

Abstract
Social determinants of health (SDH) influence emergency department (ED) use among children with asthma. We aimed to examine if SDH were more strongly associated with ED use among children with moderate/severe compared to mild asthma. This study utilized the 2016-2019 data from the National Survey of Children’s Health. Children with asthma ages 0-17 years (N=9,937) were included in the analysis. Asthma severity and all-cause ED use in the past year were reported by caregivers. The association between patient factors and ED visits was evaluated using ordinal logistic regression. Based on the study sample, 29% of children with asthma had moderate/severe asthma. In the mild group, 30% visited the ED at least once in the past 12 months, compared to 49% in the moderate/severe group. SDH associated with ED visits included race/ethnicity, insurance coverage, and parental educational attainment, but the strength of these associations did not vary according to asthma severity. In a nationally-representative data set, SDH were equally predictive of ED use regardless of children’s asthma severity. Interventions to reduce ED use among children with asthma should be considered for children with any severity of asthma, especially children in socially disadvantaged groups at higher risk of ED utilization.
WITHDRAWN: Analyzing the institutional and regulatory framework for operationalizing the blue economy in Bangladesh: Lessons from Australia
Authors: Rahman Md. Mizanur 
Published: December 2021

Abstract

This study's overarching objective was to assess the suitability and applicability of the existing legal and institutional framework for managing the coastal and marine resources in Bangladesh. Besides, the legal and institutional arrangement of Australia was examined to adopt the best practices for Bangladesh. The glimpse of potential coastal and marine resources of Bangladesh was assessed to have an idea of the untapped resources' dimensions. Together with, the fundamental challenges faced by the artisanal and small-scale fishers in Bangladesh were critically scrutinized. The empirical data collection followed a multi-approach like mini-symposium, consultation workshops, focus group discussions, case studies, visiting, personal, and key informant interviews. The study revealed that the vast provisioning, regulating, and cultural ecosystem services of the Bay of Bengal, including its coast and mangrove, is mostly unexplored. The regulatory framework in Bangladesh is characterized by jurisdictional overlapping followed by the conflict of interests among the public institutes, which originated from the aged and fragmented laws and ambiguous business allocation; consequently, the artisanal and small-scale fishers suffer a lot. On the flip side, despite some limitations, Australia established sectoral governance enacting strong legislative measures. The local government and community’s right in resource management locally has been institutionalized in Australia, which remains fuzzy in Bangladesh. Taking lessons from Australia, Bangladesh can enact new sectoral laws followed by business reallocation for the line ministries. The study will help policymakers identify the bottlenecks rooted in Bangladesh’s existing regulatory and institutional framework.
Multifunctional Flexible and Wearable Sensing System Based on Optical Microfiber Interferometry
Authors: Mishra Pratik , Kumar Hemant , Sahu Subrat , jha Rajan 
Published: December 2021

Abstract
Optical segments based flexible systems are the key for the development of futuristic advanced wearable devices for health monitoring, robotics, and ultraprecision positioning in industrial applications. Here, we have demonstrated an processed optical microfiber based multifunctional sensing system, which overcomes the various limitations of most widely reported electronics and material-based flexible devices. By optimizing the position of the post processed microfiber configuration in optimized Polydimethylsiloxane (PDMS) thickness and controlling the interference between the fundamental mode and higher order modes of microfiber to form and tunable interference pattern, we are able to make an efficient, simple, flexible and economical optical wearable vector bending system with a sensitivity as high as 1.01nm/degree. In addition, this skinmountable sensing sensor shows a remarkable and ultrasensitivity of -3.07 nm/oC. This ultrahigh sensitivity, mechanical robustness, with the excellent flexible and biocompatible nature also makes this sensing system a dominant candidate for wearable medical devices for elder-care facilities, physioclogical monitoring, athletic training, and rehabilitation program.
The Quantitative Evaluation of the Political Cost of Environmental Issues: A Case Study of a Typical Public Protest in China
Authors: Li Xintao , Cheng Tongshun , Zhang Zaisheng , Liu Li 
Published: December 2021

Abstract
It is of great reference significance for broadening the research perspective of environmental issues, improving the efficiency of government environmental governance and the credibility of the government, to scientifically measure and analyze the political cost of environmental issues. This article takes the typical case “protest event of power generation project of R steel plant in T city, China” as the research background. First, the generation process and action mechanism of the political cost of environmental issues in the actual situation are investigated. Then, through in-depth interview, multi-case grounded theory and fuzzy subordinate function analysis, the scientific construction of the political cost index system of environmental issues are completed. Finally, based on G1 method/entropy method combined with weighting and fuzzy comprehensive evaluation method, the political cost of the protest events of R iron and steel plant in T city is measured. The results show that (1) it is important that good single dimensions and reliable indicators are embodied in the overall political cost scale. Among them, the behavioral political cost of the masses is the largest proportion of all indicators; (2) after the entire environmental mass incident is over, the political costs are difficult to repair, and some lagging ideas and behaviors shown by local governments lead to a continuous expansion of the political cost associated with environmental issues; and (3) local governments should not conceal information asymmetry. Instead, local governments should give greater freedom to other actors to deal with environmental problems. This will mitigate the effect of political costs. Corresponding policy recommendations are proposed.
A Randomized Controlled Trial Enhancing Non-Communicable Diseases Health Literacy of Indian Youth
Authors: Shah Ankita R. , Jindal Rahul M , Subramanyam Malavika A. 
Published: December 2021

Abstract
Background

There is a theory-praxis gap related to health literacy interventions focused on non-communicable diseases (NCD) among young people. We designed a NCD curriculum and investigated its’ effect on health literacy in non-medical, non-nursing college students in India. We deliberately selected non-medical and non-nursing college students as we hypothesized they would have minimal baseline knowledge of NCDs.
Methods

We initially carried out a pilot study in 85 students in a four-day long workshop (12 teaching hours) using empirically developed health literacy instrument. We administered the curriculum to 120 randomly selected students in 4 colleges, while 50 students were controls. The curriculum was given over 4 days for a total of 32 hours. Each lecture comprised of didactic lecture followed by discussion and skills testing of measuring BP and blood sugar. Health literacy was measured using a specifically designed tool at baseline and endline. Difference in health literacy scores between the two time-points was analyzed using the t-test. Multiple linear and Poisson regression models were used for covariates.
Results

Study groups were comparable at baseline. The intervention group showed 20.6% higher health literacy score at endline versus control group (p<0.001). Participants scoring 40% or above on the health literacy measure in both groups were comparable at baseline. However, the proportion of participants scoring 40% or above was higher in the intervention group versus control group at endline (p<0.001).
Conclusions

We provide empirical data to support incorporation of NCDs as a credit course in college curricula in low and middle income countries. This is the first study in the Indian context that addressed NCD-related health literacy in a randomized trial.
A Socio-spatial Approach to the First Legal Hall Cohousing Setting in Switzerland: The Case Study of Hallenwohnen in Zurich
Authors: Khatibi Maryam 
Published: December 2021

Abstract
This study explores the collective settings of Hallenwohnen (hall dwelling) as a section of the Zollhaus settlement, which is the follow-up project by the Kalkbreite housing cooperative and has been in function since January 2021 in Zurich, Switzerland. Hallenwohnen is the first legal hall cohousing arrangement in Switzerland. The private and semiprivate spaces of Hallenwohnen consist of a large open hall with collective basic structures and mobile residential towers (roll spaces) as the core concept, which offer an affordable, self-managed/self-build, collaborative coliving and coworking arrangement in the center of Zurich. The qualitative case study method was applied through semistructured interviews with occupants of Hallenwohnen, direct observations, spatial investigations and document analyses. The results reveal that the innovative socio-spatial potentials of the intermediate spaces have been activated through the participatory intentions of the community. Living as one household in a collective arrangement and multiplicity usage of hybrid spaces have activated participation-capable housing spaces, while constraints and conflicts have mobilized the (re)negotiations and reinterpretations of collective housing spaces.
Rainfall distribution and tea production in Bangladesh: Socio-economic impact analysis
Authors: Rahman Md. Mizanur 
Published: December 2021

Abstract

The study aimed to assess the present pattern of tea production and the impacts of rainfall patterns on tea yield and made tea quality. The cost of production and profit-loss were examined to link with rainfall distribution. Likewise, the daily income of permanent workers, the job market of the contractual workers, and the workers' associated welfare were critically analyzed to understand the social impact. The Rangpur division showed an incremental dryness; in contrast, the Sylhet division showed a steady pattern with a high frequency of continuous heavy rainfall. The rainfall pattern of the Chottogram division was highly unpredictable and oscillated. Long spell drought and heavy showers followed by flash floods affect tea yield. According to respondents' perception, the rainfall pattern showed changing behaviours that are not favourable for tea production. The emergence of new pests and deterioration of soil characteristics were reported. Bangladesh enjoyed the most pleasant weather for tea production in 2019. The cost of production increased with the adverse weather, which increases the loss for the growers. Profit loss of growers is a determinant of the welfare of the workers impacting their livelihoods. The organic tea garden is not affected by climatic events. Consequently, the socio-economic conditions of the organic garden are safeguarded. Hence, the study recommends following organic farming to cope with climate change. Differently, it was reported that the intermediaries' hegemony in the market chain is more problematic than climate change. Policy initiatives are warranted to correct the market and to establish growers and consumers' rights.