bluuebunny/crossref_metadata_2025_split huggingface dataset:
Integration of Transcriptomic and Proteomic Data Reveals the Possible Action Mechanism of the Antimicrobial Zhongshengmycin AgainstDidymella segeticola, the Causal Agent of Tea Leaf Spot
Authors: Ren Yafeng , Li Dongxue , Jiang Shilong , Wang Yu , Tang Qin , Huang Honglin , Wang Delu , Song Baoan , Chen Zhuo 
Published: April 2021

Tea leaf spot, caused by the fungal phytopathogen Didymella segeticola, is an important foliar disease that can cause huge losses in the production and quality of tea, and there are no effective management measures to control the disease. This study screened a natural antimicrobial chemical for its activity against D. segeticola and studied its mode of action. Antifungal activity of the Streptomyces-derived antimicrobial zhongshengmycin (ZSM) against D. segeticola strain GZSQ-4 was assayed in vitro via the mycelial growth rate method. Optical microscopy and scanning and transmission electron microscopy were used to observe the morphological effects on hyphae treated with ZSM, with these studies complemented by transcriptomic, proteomic, and bioinformatic studies to identify the differentially expressed genes or differentially expressed proteins in hyphae treated with ZSM. Correlation analysis of transcriptomic and proteomic data were used to reveal the mode of action. The results indicated that ZSM could inhibit the growth of hyphae in vitro with a half-maximal effective concentration of 5.9 μg/ml, inducing some morphological changes in organelles, septa, and extracellular polysaccharides, targeting ribosomes to disturb translation, affecting the biosynthesis of some hyphal proteins at the messenger RNA and protein levels, and revealing correlations between findings from transcriptomes and proteomes.
Validation of Quantitative and Digital Polymerase Chain Reaction Assays Targeting the Mating Types of Phyllosticta citricarpa, the Causal Agent of Citrus Black Spot
Authors: Urbina Hector , Smith Taylor , Jones Callie , Sun Xiaoan , McVay John , Walker Caroline , Whilby Leroy , Hodges Greg , Smith Trevor 
Published: April 2021

Citrus black spot (CBS) is a disease caused by the ascomycetous fungus Phyllosticta (formerly Guignardia) citricarpa (Botryosphaeriales, Pezizomycotina) currently present in citrus groves in five counties in southwest Florida. Within Florida, P. citricarpa shows limited reproduction via asexual sporulation due to the presence of only one (MAT1-2-1) of the two required mating types for sexual reproduction. Here, we present two novel PCR assays standardized in quantitative PCR (qPCR) and digital PCR (dPCR) platforms to distinguish both mating types (MAT1-1-1 and MAT1-2-1) of P. citricarpa to monitor for the potential introduction of the MAT1-1-1 mating type into Florida and a novel protocol for DNA extraction from asymptomatic leaves. During citrus harvesting season 2018 − 19, fruit lesions as well as asymptomatic leaves adjacent to symptomatic fruit and asymptomatic trees in CBS-infected groves were surveyed for P. citricarpa presence and mating types. Results support the presence of only the MAT1-2-1 mating type in Florida, after surveying more than 1,145 citrus fruit lesions. We also confirmed the limited dispersal ability of the asexual state of P. citricarpa in Florida in 10 groves using the enhanced capabilities of the dPCR platform in the detection of P. citricarpa directly from asymptomatic leaves with low pathogen inoculum. [Formula: see text] Copyright © 2021 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license .
The Midlife Transition and the Risk of Cardiovascular Disease and Cancer Part II: Strategies to Maximize Quality of Life and Limit Dysfunction and Disease
Authors: Kase Nathan G. , Friedman Elissa Gretz , Brodman Michael 
Published: April 2021

(Abstracted from Am J Obstet Gynecol 2020;223:834–847.e2)
The leading causes of death in the United States are heart disease and cancer. Among women, the clinical appearance of heart disease and cancer (breast, endometrium, and ovary) escalates during the decades of the midlife transition during and after menopause.
Medication-Assisted Treatment vs. Detoxification for Women Who Misuse Opioids in Pregnancy: Associations With Dropout, Relapse, Neonatal Opioid Withdrawal Syndrome (NOWS), and Childhood Sexual Abuse
Authors: Macfie Jenny , Towers Craig V. , Fortner Kimberly B. , Stuart Gregory L. , Zvara Bharathi J. , Kurdziel-Adams Gretchen , Kors Stephanie B. , Noose Samantha K. , Gorrondona Andrea M. , Cohen Chloe T. 
Published: April 2021

(Abstracted from Addict Behav Rep 2020;12:100315)
The American College of Obstetrics and Gynecology recommends medically assisted treatment (MAT) for the management of women who misuse opioids during pregnancy. Medically assisted treatment allows pregnant women to use opioids at doses that lessen the euphoric effect, minimize withdrawal symptoms, and curb cravings.
Maternal Perceived Discrimination and Association With Gestational Diabetes
Authors: MacGregor Caitlin , Freedman Alexa , Keenan-Devlin Lauren , Grobman William , Wadhwa Pathik , Simhan Hyagriv N. , Buss Claudia , Borders Ann 
Published: April 2021

(Abstracted from Am J Obstet Gynecol MFM 2020;2:100222)
Up to 7% of pregnancies are complicated by gestational diabetes mellitus (GDM), disproportionately affecting women of color and lower socioeconomic status. These disparities in GDM have been associated with risk factors such as obesity and chronic stress.
Clinical Outcomes of a Genomic Screening Program for Actionable Genetic Conditions
Authors: Buchanan Adam H. , Kirchner H. Lester , Schwartz Marci L. B. , Kelly Melissa A. , Schmidlen Tara , Jones Laney K. , Hallquist Miranda L. G. , Rocha Heather , Betts Megan , Schwiter Rachel , Butry Loren , Lazzeri Amanda L. , Frisbie Lauren R. , Rahm Alanna Kulchak , Hao Jing , Willard Huntington F. , Martin Christa L. , Ledbetter David H. , Williams Marc S. , Sturm Amy C. 
Published: April 2021

(Abstracted from Genet Med 2020;22:1874–1882)
Hereditary breast and ovarian cancer (HBOC), Lynch syndrome, and familial hypercholesterolemia (FH) are recognized by the Centers of Disease Control and Prevention as tier-1 genetic conditions for which early identification and management could decrease morbidity and mortality. Yet few patients with a personal or familial history of these conditions are screened for genetic risk.
Functional Outcomes for Meniscal Allograft Interposition Arthroplasty of the Hand
Authors: McCullough Meghan C. , Minasian Raquel , Tanabe Kylie , Rodriguez Sandra , Kulber David 
Published: April 2021

Background:
Osteochondral defects of the carpometacarpal (CMC), metacarpophalangeal (MCP), and proximal interphalangeal (PIP) joints often necessitate arthrodesis or arthroplasty. Meniscal allograft has been used for large joint resurfacing, but its application to smaller joints is less well understood. We propose its use for hand joint resurfacing as an off-the-shelf alternative to address osteochondral defects and restore articular function.

Methods:
Thirty-one patients with osteoarthritis of the CMC, MCP, or PIP joints underwent arthroplasty with meniscal allograft. Patient demographics and operative information were recorded. Preoperative Disability of the Shoulder, Arm, and Hand, Wong Baker pain, grip and pinch strength, and range of motion were compared to postoperative scores at 6 weeks, 6 months, and 1 year.

Results:
Twenty-three women and 8 men, mean age 62.8 years, underwent 39 joint reconstructions, including CMC (n = 26), thumb MP (n = 2), thumb IP (n = 2), digit MP (n = 2), and digit PIP (n = 7). At 1 year, mean Disability of the Shoulder, Arm, and Hand scores decreased from 41.3 to 15.6 (P < 0.001) and pain scores from 6.9 to 1.0 (P < 0.001). Grip strength increased from 38.1 to 42.9 (P = 0.017) and radial and palmar abduction from 43.1 to 49.2 (P = 0.039) and 43.7 to 51.6 (P = 0.098), respectively. There were no complications related to the meniscus.

Conclusions:
Meniscal allograft represents an alternative to arthrodesis which obviates the need for a donor site and avoids many of the complications inherent to synthetic alternatives. Our early results demonstrate its successful use to reduce subjective pain and disability scores, improve objective strength measures, and maintain range of motion.
Bilateral Pseudomonas aeruginosa Keratitis in 7 Patients
Authors: Perzia Brittany , Enzor Rikki , Kowalski Regis P. , Jhanji Vishal 
Published: April 2021

Purpose:
To report 7 patients (14 eyes) with bilateral Pseudomonas aeruginosa keratitis (PAK).

Methods:
Case records of patients with bilateral PAK were reviewed at a single academic medical center from April 2009 to December 2020.

Results:
Seven patients aged 29 to 94 years presented with bilateral P. aeruginosa corneal ulcers. All patients were soft contact lens wearers except one elderly patient with a complex ocular history. Three patients wore specialty contact lens, including one cosmetic contact lens wearer, one multifocal contact lens wearer, and one extended-wear contact lens wearer. The remaining three patients were not overnight contact lens wearer but regularly showered in contact lens or used tap water for contact lens case cleaning. All patients presented with asymmetric disease, with visual acuity ranging from 20/20 to light perception. Ulcers were located centrally in 5 eyes (35.7%), diffusely over the entire cornea in 5 eyes (35.7%), temporally in 2 eyes (14.2%), and nasally in 1 eye (7.14%), and in 1 eye (8.3%), the ulcer location was not recorded. Corneal thinning was noted in 7 eyes (50%). Hypopyon was also noted in 7 eyes (50%). Two patients required procedures because of progressive stromal necrosis. All other patients were treated nonsurgically, with antibiotic drops, which resulted in ulcer resolution. Final visual acuity on last recorded follow-up ranged from 20/20 to no light perception.

Conclusions:
This is the largest case series of bilateral PAK, which occurred primarily in contact lens wearer. This case series reiterates the risk of contact lens infection related to poor hygiene.
A meta-analysis of optimal medical therapy with or without percutaneous coronary intervention in patients with stable coronary artery disease
Authors: Shah Rahman , Nayyar Mannu , Le Francis K , Labroo Ajay , Nasr Abrar , Rashid Abdul , Davis Donnie A , Weintraub William S. , Boden William E. 
Published: April 2021

Background
Whether percutaneous coronary intervention (PCI) improves clinical outcomes in patients with chronic angina and stable coronary artery disease (CAD) has been a continuing area of investigation for more than two decades. The recently reported results of the International Study of Comparative Health Effectiveness with Medical and Invasive Approaches, the largest prospective trial of optimal medical therapy (OMT) with or without myocardial revascularization, provides a unique opportunity to determine whether there is an incremental benefit of revascularization in stable CAD patients.

Methods
Scientific databases and websites were searched to find randomized clinical trials (RCTs). Pooled risk ratios were calculated using the random-effects model.

Results
Data from 10 RCTs comprising 12 125 patients showed that PCI, when added to OMT, were not associated with lower all-cause mortality (risk ratios, 0.96; 95% CI, 0.87–1.08), cardiovascular mortality (risk ratios, 0.91; 95% CI, 0.79–1.05) or myocardial infarction (MI) (risk ratios, 0.90; 95% CI, 0.78–1.04) as compared with OMT alone. However, OMT+PCI was associated with improved anginal symptoms and a lower risk for revascularization (risk ratios, 0.52; 95% CI, 0.37–0.75).

Conclusions
In patient with chronic stable CAD (without left main disease or reduced ejection fraction), PCI in addition to OMT did not improve mortality or MI compared to OMT alone. However, this strategy is associated with a lower rate of revascularization and improved anginal symptoms.
Development of delayed neurologic sequelae in acute carbon monoxide poisoning cases caused by briquette-based kotatsu
Authors: Onodera Makoto , Tsukada Yasuhiko , Suzuki Tsuyoshi , Sorimachi Kotaro , Ebihara Kenichi , Sato Lubna , Zenda Rie , Ueno Satoshi , Sugaya Kazuki , Iseki Ken 
Published: April 2021

Abstract
Briquette-based kotatsu, a traditional Japanese heating system, is still used in rural areas and has been linked to the development of acute carbon monoxide (CO) poisoning. This study aimed to investigate the occurrence of delayed neurologic sequelae (DNS) in patients with acute CO poisoning caused by briquette-based kotatsu.
This retrospective study included 17 patients treated for acute CO poisoning due to briquette-based kotatsu, between April 2017 and March 2020. Patients were divided into either a sequelae group (3 patients) or a non-sequelae group (14 patients) based on the presence or absence, respectively, of DNS. Demographic data, kotatsu characteristics, clinical findings, and therapies were compared between the 2 groups.
Significant differences were noted in patient posture during their initial discovery. Specifically, all non-sequelae patients only had their legs under the kotatsu quilt and all sequelae patients had their entire bodies under the kotatsu quilt (P = .001). There were no statistically significant differences in carbon monoxide levels in hemoglobin (CO-Hb) or the creatine-kinase myocardial band (CK-MB), between the 2 groups; however, troponin-I levels were significantly higher in the sequelae group (P = .026). Abnormal head imaging findings were noted in 2 sequelae-group patients, with a significant difference between the groups (P = .025).
We speculate that acute CO poisoning, caused by briquette-based kotatsu, may lead to DNS more frequently in patients in who cover their entire body with the kotatsu quilt and are found in this position. Patients should be warned about the dangers of acute CO poisoning when using briquette-based kotatsu.
Primary varicella-zoster virus infection of the immunocompromised associated with acute pancreatitis and hemophagocytic lymphohistiocytosis
Authors: Bérar Antoine , Ardois Samuel , Walter-Moraux Patricia , Jegonday Marc-Antoine , Henriot Basile 
Published: April 2021

Abstract

Rationale:
Primary varicella-zoster virus (VZV) infection may be associated with hemophagocytic lymphohistiocytosis (HLH), as well as with acute pancreatitis. However, there is few data concerning the evolution and the optimal treatment of these rare associations.

Patient concerns:
A 57-year-old immunocompromised woman, who was treated for chronic lymphocytic leukemia 3 years prior to admission, was hospitalized with abdominal pain revealing severe acute pancreatitis. The day after admission, a pruritic rash appeared on her face, trunk, and limbs, sparing the palmoplantar regions. At the same time, fever, thrombocytopenia (27 × 109/L), major hyperferritinemia (11,063 μg/mL), hypertriglyceridemia (2.56 mmol/L) and elevated lactate dehydrogenase levels (1441 IU/L) suggested HLH.

Diagnosis:
The diagnosis of chickenpox (varicella) was established. Primary VZV infection was then confirmed: cutaneous and plasma VZV polymerase chain reactions were positives, VZV serology was negative for IgG.

Interventions:
Treatment with aciclovir was started intravenously after the onset of the rash, for a total of 10 days. A 48-h surveillance in intensive care was carried out.

Outcomes:
Acute pancreatitis and biological abnormalities evolved favorably under aciclovir. Platelet count was normalized 6 days after admission to hospital.

Lessons:
A favorable outcome of primary VZV infection associated with severe acute pancreatitis and probable HLH in an immunocompromised patient is possible with aciclovir alone.
The efficacy and safety of cupping therapy for treating of intractable peripheral facial paralysis
Authors: Cao Zhiwen , Jiao Lin , Wang Hongyu , Li Jun , Zhong Genping , Zhu Daocheng , Xu Wei , Jin MengKe 
Published: April 2021

Abstract

Background:
Peripheral facial paralysis (PFP) is a common clinical neurological disease and the incidence of intractable peripheral facial paralysis is on the rise. Symptoms include crooked mouth and eyes, tearing and shallow nasolabial folds. The disease seriously affects the physical and mental health of patients. At present, a large number of clinical studies have shown that cupping is effective in treating intractable peripheral facial paralysis (IPFP). Therefore, the purpose of this review is to evaluate the effectiveness and safety of cupping in the treatment of refractory peripheral facial paralysis.

Methods:
We will conduct a comprehensive and systematic search of relevant documents in the following databases: Medline, PubMed, the Cochrane Central Register of Controlled Trials (CENTRAL), Embase, Chinese Biomedical Literatures Database, China National Knowledge Infrastructure (CNKI), Wang Fang Database, Chinese Scientific Journal Database from inception to February 2021 without any language restriction. The 2 reviewers will be independently completed select research, extract data, evaluate research quality and use the Cochrane risk of bias tool to assess methodological quality. Using revman5.4 software for statistical analysis. The degree of heterogeneity will be Determined through heterogeneity test, to definite whether to adopt a random effects model or a fixed-effects model.

Results:
The protocol for the meta-analysis will systematically evaluate the efficacy and safety of cupping therapy for intractable peripheral facial paralysis patients.

Conclusion:
This study will explore whether or not cupping therapy can be used as one of the non-drug therapies to prevent or treat intractable peripheral facial paralysis
A meta-analysis of the secondary fractures for osteoporotic vertebral compression fractures after percutaneous vertebroplasty
Authors: Zhai Gongwei , Li Ang , Liu Binfeng , Lv Dongbo , Zhang Jingyi , Sheng Weichao , Yang Guang , Gao YanZheng 
Published: April 2021

Abstract
To identify the risk factors of the secondary fractures for osteoporotic vertebral compression fractures (OVCFs) after percutaneous vertebroplasty (PVP).
We conducted a search of relevant articles using Cochrane Library, PubMed, Medline, Science Direct, Embase, the Web of Science and other databases. The time range we retrieved from establishment of the electronic database to November 2017. Gray studies were found in the references of included literature reports. STATA version 11.0 (Stata Corporation, College Station, Texas) was used to analyze the pooled data.
Fourteen studies involving 1910 patients, 395 of whom had fracture secondary to the surgery were included in this meta-analysis. The result of meta-analyses showed the risk factors of the secondary fractures for OVCFs after PVP was related to bone mineral density (BMD) [95%CI (−0.650, −0.164), SMD=−0.407, P=.001], cement leakage ((RR=0.596, 95%CI (0.444,0.798), P = .001)), and kyphosis after primary operation ((SMD=0.741, 95%CI (0.449,1.032), P = .000)), but not to gender, age, body mass index, cement volume, thoracolumbar spine, and cement injection approaches.
Bone mineral density, cement leakage, and kyphosis after primary operation are the risk factors closely correlative to the secondary fracture after PVP. There have not been enough evidences to support the association between the secondary fracture and gender, age, body mass index, cement volume, thoracolumbar spine, and cement injection approaches.
Correlation between real-time heart rate and fatigue in chest compression providers during cardiopulmonary resuscitation
Authors: Bae Go Eun , Choi Arom , Beom Jin Ho , Kim Min Joung , Chung Hyun Soo , Min In Kyung , Chung Sung Phil , Kim Ji Hoon 
Published: April 2021

Abstract

Background:
The American Heart Association guidelines recommend switching chest compression providers at least every 2 min depending on their fatigue during cardiopulmonary resuscitation (CPR). Although the provider's heart rate is widely used as an objective indicator for detecting fatigue, the accuracy of this measure is debatable.

Objectives:
This study was designed to determine whether real-time heart rate is a measure of fatigue in compression providers.

Study design:
A simulation-based prospective interventional study including 110 participants.

Methods:
Participants performed chest compressions in pairs for four cycles using advanced cardiovascular life support simulation. Each participant's heart rate was measured using wearable healthcare devices, and qualitative variables regarding individual compressions were obtained from computerized devices. The primary outcome was correct depth of chest compressions. The main exposure was the change in heart rate, defined as the difference between the participant's heart rate during individual compressions and that before the simulation was initiated.

Results:
With a constant compression duration for one cycle, the overall accuracy of compression depth significantly decreased with increasing heart rate. Female participants displayed significantly decreased accuracy of compression depth with increasing heart rate (odds ratio [OR]: 0.97; 95% confidence interval [CI]: 0.95–0.98; P < .001). Conversely, male participants displayed significantly improved accuracy with increasing heart rate (OR: 1.03; 95% CI: 1.02–1.04; P < .001).

Conclusion:
Increasing heart rate could reflect fatigue in providers performing chest compressions with a constant duration for one cycle. Thus, provider rotation should be considered according to objectively measured fatigue during CPR.
Efficacy and safety of Biqi capsule in the treatment of knee osteoarthritis
Authors: Zhou YaZhou , Wang WenGang , Tian Ke , Huang Hui , Jia Mengrui 
Published: April 2021

Abstract

Background:
Knee osteoarthritis (KOA) is a chronic and degenerative bone and joint disease, with KOA, cartilage degeneration, destruction and subchondral bone remodeling as the main pathological features. Its clinical symptoms are knee pain, swelling, limited activity, and long course of disease can cause joint deformities. At present, the early treatment of Western medicine is mainly the use of nonsteroidal drugs for anti-inflammation and removing pain, but because the efficacy of these drugs is unstable, the disease is easy to repeat after treatment, and the clinical effect is not good. Although Biqi capsule has advantages in the treatment of KOA, there is a lack of standard clinical studies to verify it, so the purpose of this randomized controlled study is to evaluate the efficacy and safety of Biqi capsule in the treatment of KOA.

Methods:
This is a prospective randomized controlled trial to study the efficacy and safety of Biqi capsule in the treatment of KOA. The patients were randomly divided into a treatment group and a control group according to 1:1. Among them, treatment group: Biqi capsule combined with diclofenac sodium sustained release tablets; Control group: Diclofenac sodium sustained-release tablets alone. Both groups were treated with standard treatment for 2 weeks and were followed up for 30 days to pay attention to the efficacy and safety indexes. Observation indicators included: the Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC), Hospital for Special Surgery Knee Score (HSS), liver and kidney function, adverse reactions, and so on. SPSS 25.0 software is used for data analysis.

Discussion:
This study will evaluate the efficacy and safety of Biqi capsule in the treatment of KOA, and the results of this experiment will provide a clinical basis for Biqi capsule in the treatment of KOA.

Trial registration:
OSF Registration number: DOI 10.17605/OSF.IO/6HB9D