bluuebunny/crossref_metadata_2025_split huggingface dataset:
Nicotinamide Mononucleotide Adenylyltransferase 1 Regulates Cerebral Ischemia-Induced Blood–Brain Barrier Disruption Through NAD+/SIRT1 Signaling Pathway
Authors: Zhang Yang , Guo Xun , Peng Zhifeng , Liu Chang , Ren Lili , Liang Jia , Wang Peng 
Published: November 2021

Abstract
The molecular mechanisms of blood–brain barrier (BBB) disruption in the early stage after ischemic stroke are poorly understood. In the present study, we investigated the potential role of nicotinamide mononucleotide adenylyltransferase 1 (NMNAT1) in ischemia-induced BBB damage using an animal middle cerebral artery occlusion (MCAO) model of ischemic stroke. Recombinant human NMNAT1 (rh-NMNAT1) was administered intranasally and Sirtuin 1 (SIRT1) siRNA was administered by intracerebroventricular injection. Our results indicate that rh-NMNAT1 reduced infarct volume, improved functional outcome and decreased BBB permeability in mice after ischemic stroke. Furthermore, rh-NMNAT1 prevented the loss of tight junction proteins (occludin and claudin-5) and reduced cell apoptosis in ischemic microvessels. NMNAT1-mediated BBB permeability was correlated with the elevation of nicotinamide adenine dinucleotide (NAD+)/NADH ratio and SIRT1 level in brain microvascular endothelial cells. In addition, rh-NMNAT1 treatment significantly decreased the levels of acetylated nuclear factor-κB, acetylated p53 and matrix metalloproteinase-9 in ischemic microvessels. Moreover, the protective effects of rh-NMNAT1 could be reversed by SIRT1 siRNA. In conclusion, these findings indicate that rh-NMNAT1 protects BBB integrity after cerebral ischemia via the NAD+/SIRT1 signaling pathway in brain microvascular endothelial cells. NMNAT1 may be a novel potential therapeutic target for reducing BBB disruption after ischemic stroke.
The Comparison of the Dietary Intake Loss Between Elderly and Non-elderly Patients after Gastrectomy for Gastric Cancer
Authors: Nakazono Masato , aoyama toru , Komori Keisuke , Watanabe Hayato , kano kazuki , Nagasawa Shinsuke , kenki segami , tamagawa hiroshi , rino yasushi , Ogata Takashi , oshima takashi 
Published: November 2021

Abstract
Background/Aim: The present study quantified the changes in the dietary and nutritional intake after gastrectomy between elderly and non-elderly patients. Patients and Methods: This prospective observational study enrolled patients who underwent curative gastrectomy for gastric cancer. Results: 23 patients ≥75 years old were classified into the elderly group, and127 patients <75 years old were classified into the non-elderly group. The respective median % dietary intake losses at one and three months postoperatively were -12.4% and -5.3% in the elderly group and -8.3% and -2.8% in the non-elderly group (p=0.075 and 0.080). On comparing the intake loss of three major nutrients, the respective median % lipid intake losses at 1 and 3 months postoperatively were -13.5% and -5.8% in the elderly group and -7.3% and 0% in the non-elderly group (p=0.029 and 0.045). Conclusion: Our results suggested that elderly patients experienced more serious lipid intake loss after gastrectomy than non-elderly patients.
Hybrid Optimization-Based Robust Watermarking Using Denoising Convolutional Neural Network
Authors: Mahto Dhiran Kumar , Singh Amit 
Published: November 2021

Abstract
Colour images have been widely used in many aspects of life; however, copyright violation issues related to these images motivate research efforts. This paper aims to develop an enhanced watermarking algorithm for producing a watermarked image using hybrid optimisation with high imperceptibility and robustness. The algorithm is based on spatial and transform domains and begins by embedding multiple secret marks into cover media using an optimal scaling factor. The multi-type mark contributes an additional level of authenticity to the proposed algorithm. Furthermore, the marked image is encrypted using an improved encryption scheme, and the denoising convolutional neural network (DnCNN) is employed to enhance the robustness of the proposed algorithm. The results reveal that the proposed watermarking algorithm yields low computational overhead, excellent watermark capacity, imperceptibility, and robustness to common filtering attacks. Moreover, the comparison shows that the proposed algorithm outperforms other competing methods.
Nocturia as a Risk Factor for All-Cause and Cardiovascular Disease Mortality
Authors: Moon Shinje , Kim Yoon Jung , Chung Hye Soo , Yu Jae Myung , Park Il In , Park Sung Gon , Pak Sahyun , Kwon Ohseong , Lee Young Goo , Cho Sung Tae 
Published: November 2021

Abstract
This study aimed to evaluate the relationship between nocturia and mortality risk using the National Health and Nutrition Examination Survey database 2005–2010, given that only few studies have investigated nocturia or its association with mortality using this database. Data were obtained from the database, and nocturia was defined based on the symptom questionnaire. We categorized patients into two groups: mild nocturia (2–3 voids/night) and moderate-to severe nocturia (≥4 voids/night). Mortality data were obtained by linking the primary database to death certificate data found in the National Death Index with mortality follow-up up to December 31, 2015. Multiple Cox proportional hazard regression analyses were performed with adjustment for confounding variables at the baseline survey. We included 9,892 adults (4,758 men, 5,134 women) in this study. Nocturia occurred in 3,314 individuals (33.5%). In the multiple Cox regression analysis (results presented as hazard ratio, 95% confidence interval), nocturia was significantly associated with all-cause (1.21, 1.08–1.35, p=0.001) and cardiovascular disease (1.45, 1.13–1.85, p=0.003) mortality. Both mild and moderate-to-severe nocturia were significantly associated with all-cause (1.14, 1.02–1.28, p=0.021 and 1.62, 1.34–1.98, p<0.001, respectively) and cardiovascular disease (1.43, 1.11–1.84, p=0.006 and 1.58, 1.01–2.45, p=0.043, respectively) mortality. Nocturia was significantly associated with mortality in men and women after adjustments for major confounding factors. Moreover, the mortality risk increased with increasing nocturia severity.
Biodiversity Hotspots for Conservation of Hancornia speciosa GOMES
Authors: Álvares-Carvalho Sheila Valéria , Vieira Thiago Roberto Soares , de Freitas Bruno Antonio Lemos , de Souza Erica Moraes Santos , Gomes Laura Jane , MANN RENATA SILVA 
Published: November 2021

Abstract
Hancornia speciosa is the target of research on genetic diversity, ethnobotanical and medicinal studies. However, information on the genetic variability of populations associated with modeling the potential distribution in the state of Sergipe has not yet been performed. The objective of this study was to predict the potential occurrence of H. speciosa in areas of high use of their fruits. The maximum entropy method was used to detect the distribution patterns of H. speciosa in variable environments. The diversity of four natural populations, situated in areas of extractivist, was determined by ISSR molecular markers. The species occurs more densely in the coastal regions of Sergipe. The prediction of occurrence indicates that the species reduces areas of occurrence, mainly due to anthropic actions. It is suggested that the species needs public policies aimed at its conservation and the priority populations for conservation.
Large cooperativity in strongly coupled chip-scale photonic-atomic integrated system
Authors: Naiman Alex , Sebbag Yoel , Talker Eliran , Barash Yefim , Stern Liron , Levy Uriel 
Published: November 2021

Abstract
The miniaturization of atomic quantum systems and their integration into silicon microchips paves the way for a wide variety of applications in quantum computing, metrology and magnetometry. A particular interest is found in the integration of quantum entities into the micro and nanoscale photonic resonators to implement chip scale cavity quantum electrodynamics. Here we demonstrate the interaction of a chip scale micro disc resonator with thermal rubidium atoms via the evanescent field of the mode. We observe high Rabi splitting of 4 GHz in the transmission spectrum of the coupled photonic-atomic system due to collective enhancement of the coupling rate by the ensemble of hot atoms and present a theoretical model to support the measured results. This result corresponds to atom-photon cooperativity of ~ 1. Such cooperativity is the onset for quantum interference, needed for high-end chip scale quantum technologies, such as such as quantum manipulation, quantum information storage and processing, and few photon switching.
GYY4137 Ameliorates Sepsis-induced Cardiomyopathy via Inhibiting NLRP3 Inflammasome Activity and Reduction of Oxidative Stress in the Myocardium
Authors: Tao zhou , Qian Huitao , Zheng Nan , lu qiulun , Han Yi 
Published: November 2021

Abstract
BACKGROUNDSepsis-induced cardiomyopathy often leads to bad prognosis of patients, and even death. However, there is no effective therapeutic strategies for sepsis-caused cardiomyopathy. GYY4137 has positive therapeutic effects in many diseases. The aim of this study was to explore the mechanism underlying the sepsis-induced cardiomyopathy and investigate the protective role of the hydrogen sulfide (H2S) donor GYY4137 in sepsis-induced cardiomyopathy.METHODSGYY4137 was administrated in mouse model of sepsis-induced cardiomyopathy (SICM) was generated by cecum ligation and puncture (CLP), and then plasma levels of hydrogen sulfide and cytokines were measured, and inflammatory cell infiltration in myocardial tissue was determined by immunohistochemistry and immunofluorescence staining. RNA sequencing assay was performed to monitor the RNA expression profile. RESULTSH2S levels were reduced in the serum of sepsis-molded mice, GYY4137 reduced macrophage infiltration in the sepsis model. RNA-seq suggested that GYY4137 has an important role in inflammatory regulation. Hydrogen sulfide can inhibit NLRP3 inflammasome activity in macrophages and reduce the secretion of inflammatory factors, while GYY4137 can reduce the production of reactive oxygen species in cardiomyocytes, thus exerting a protective effect on the myocardium.CONCLUSIONGYY4137 can reduce myocardial injury in sepsis by inhibiting the inflammatory response and reducing the production of myocardial reactive oxygen species. It can provide new therapeutic ideas for clinical practice.
Polyhydroxyalkanoate Recovery From Newly Screened Bacillus Sp. LPPI-81 Using Various Methods of Extraction From Loktak Lake Sediment Sample
Authors: Ebu Seid Mohammed , Ray Lopamudra 
Published: November 2021

Abstract
Nowadays the conventional plastic wastes are very challenging to environments and its production cost also creates an economic crisis due to petrochemical-based plastic. In order to solve this problem, the current studies were aimed at screening and characterizing these PHA producing isolates and evaluating the suitability of some carbon source for newly screened PHA producing isolates. Some carbon sources such as D-fructose, glucose, molasses, D-ribose and sucrose were evaluated for PHA production. Data were analyzed using SPSS version 20. The 16SrRNA gene sequence of these isolates was performed. This newly isolated taxa was related to Bacillus species. It was designed as Bacillus sp. LPPI-18 and affiliated Bacillus cereus ATCC 14577T (AE01687) (99.10%). Paenibacillus sp. 172 (AF273740.1) was used as an out-group. Bacillus sp. LPPI-18 is a gram-positive, rod-shaped, endospore former, and citrate test positive. This isolate showed positive for amylase, catalase, pectinase, and protease test. They produced intracellular PHA granules when this isolate was stained with Sudan Black B (SBB) and Nile Blue A (NBA) preliminary and specific staining dyes, respectively. Both Temperature and pH used to affect PHA productivity. Bacteria are able to reserve PHA in the form of granules during stress conditions. This isolate produces only when supplied with carbon sources. More PHA contents (PCs) were obtained from glucose, molasses, and D-fructose. In this regard, the maximum mean value of PC was obtained from glucose (40.55±0.7%) and the minimum was obtained from D-Ribose (12.4±1.4%). Great variations (p≤0.05) of PCs were observed among glucose & sucrose, molasses & sucrose and D-fructose & sucrose carbon sources for PHA productivity (PP) of Cell Dry Weight (CDW) g/L. After extraction, PHA film was produced for this typical isolate using glucose as a sole carbon source. Fourier transform infrared spectrum was performed for this isolate and showed the feature of polyester at 1719.64 to 1721.16 wavelength for these extracted samples. The peak of fingerprinting (band of carboxylic acid group) at this wave-length is a characteristic feature of PHB and corresponds to the ester functional group (C=O).
Super-Resolution Imaging of Xylem Cell Wall Nanostructure Using SRRF Microscopy
Authors: Donaldson Lloyd 
Published: November 2021

Abstract
Background: The nanostructure of plant cell walls is of significant biological and technological interest, but methods suited to imaging cell walls at the nanoscale while maintaining the natural water-saturated state are limited. Light microscopy allows imaging of wet cell walls but with spatial resolution limited to the micro-scale. Most super-resolution techniques require expensive hardware and/or special stains so are less applicable to some applications such as autofluorescence imaging of plant tissues. Results: A protocol was developed for super-resolution imaging of xylem cell walls using super-resolution radial fluctuations (SRRF) microscopy combined with confocal fluorescence imaging (CLSM). We compared lignin autofluorescence imaging with acriflavin or rhodamine B staining. The SRRF technique allows imaging of wet or dry tissue with moderate improvement in resolution for autofluorescence and acriflavin staining, and a large improvement for rhodamine B staining, achieving sub 100 nm resolution based on comparison with measurements from electron microscopy. Rhodamine B staining, which represents a convolution of lignin staining and cell wall accessibility, provided remarkable new details of cell wall structural features including both circumferential and radial lamellae demonstrating nanoscale variations in lignification and cell wall porosity within secondary cell walls. Conclusions: SRRF microscopy can be combined with confocal fluorescence microscopy to provide nanoscale imaging of plant cell walls using conventional stains or autofluorescence in either the wet or dry state.
Acupuncture For Major Depressive Disorder: A Systematic Review and Meta-Analysis of High-Quality Randomized Control Trials
Authors: Xu Guixing , Xiao Qiwei , Huang Biqing , Lei Hanzhou , Yin Zihan , Huang Liuyang , Zhou Zhuo , Tian Hao , Huang Fengyuan , Liu Yilin , Sun Mingsheng , Zhao Ling , Liang Fanrong 
Published: November 2021

Abstract
Background: The effects of acupuncture for major depressive disorder (MDD) uncertain. This review aims to determine the effects of Acupuncture for MDD .Methods: Eight Database were searched to identify randomized control trials (RCTs) on Acupuncture for MDD. All RCTs with adult participants undergoing acupuncture treatment for MDD were included. The primary outcome measure was the 24-item Hamilton rating scale for depression (HAMD-24). We used random-effects meta-analysis to synthesis the results with mean difference or odds ratio. Furthermore, the potential heterogeneity was tested through meta-regression/subgroup analyses/sensitive analysis. The quality of evidence for each outcome was assessed by the Grading of Recommendations Assessment, Development and Evaluation approach.Results: Forty-three studies were included: 9 acupuncture versus sham-acupuncture (n=920), 26 acupuncture versus antidepressants (n=2169), 9 acupuncture plus antidepressants versus antidepressants (n=667). Of the 43 high-quality articles, 24 and 8 were determined to have a low and moderate risk of bias, respectively. The pooled results for HAMD-24 and SDS revealed the clinical benefits of Acupuncture or Acupuncture plus antidepressants compared to sham-acupuncture or antidepressants, with high quality evidence. Furthermore, high quality of evidence showed that acupuncture led to fewer adverse effects compared to antidepressants.Conclusions: Acupuncture or acupuncture plus antidepressants were significantly associated with reduced HAMD-24 scores, with high-quality evidence. More rigorous trials are needed to identify the optimal frequency of Acupuncture for MDD and integrate such evidence into clinical care to reduce antidepressant use.
Knowledge, Practice, and Attitude of patients and physicians towards insulin therapy in Type 2 DM: a cross sectional study in TASH patients and Physician from various universities in Ethiopia
Authors: Sheno Biniyam Woya , Reja Ahmed 
Published: November 2021

Abstract
Background
As type 2 DM is a growing health problem worldwide early treatment and intensification of therapy will decrease the vascular complications as evidenced by large trials. Insulin therapy is delayed in this group of patients and multiple physician and patient factors are mentioned in different settings.
Methods
This study is a cross-sectional, quantitative descriptive study conducted on 221patients at TASH endocrine clinic and 122 physicians working at TASH, residents visiting for detachment, and Yekatit 12 hospital. Conducted on Jul 1- Sep 30, 2020, G.C
Result
76% of physicians think taking insulin at the prescribed time is difficult while only 31.5% of patients think it’s difficult. Hypoglycemia is one of the most fearful parts of having diabetes for 81.1% of patients on Insulin therapy. 87% of physicians and76.6% of patients think insulin-treated diabetes controls life while. 56% of patients therapy know nothing about insulin therapy. 23% of patients said they will not accept insulin therapy the top reason being they will be afraid of insulin injection because they think it will be painful.
Conclusion
Physicians tend to overestimate the difficulty of insulin injection preparation and dose adjustment fears the risk of hypoglycemia when starting insulin therapy. More than half of our patients not on insulin therapy know nothing about insulin therapy which we have to improve. Even if patients not on insulin therapy overestimate insulin needle phobia and injection pain those who are already on insulin disagree with them.
C-Type Natriuretic Peptide/Natriuretic Peptide Receptor B Regulate the Expression and Secretion of Antibacterial Peptide S100A7 in Goat Mammary Gland Through PKG/JNK/c-Jun Signaling Pathway
Authors: Fan Mingzhen , Yan Yutong , Miao Yuyang , Zhu Kunyuan , Zhao Xiaoe , Pan Menghao , Ma Baohua , Wei Qiang 
Published: November 2021

Abstract
Background

C- type natriuretic peptide (CNP) and its receptor natriuretic peptide receptor B (NPR-B) were members of natriuretic peptides system which were well known produced in cardiovascular and central nervous system. However, whether CNP/NPR-B is expressed in mammary gland, and what its role in mammary gland has not been reported. The antimicrobial peptide S100A7 is a critical component of the animal innate immune system and plays important roles to resist infection of pathogens in the mastitis. It is a hypothesis that CNP/NPR-B signaling pathway maybe induces the expression and secretion of S100A7 in mammary epithelial cells to take part in local mammary gland innate immune. In order to verify this hypothesis, goat mammary gland and isolated mammary epithelial cells (MECs) were used to explore the expression of CNP/NPR-B and their physiological roles in goat mammary gland.
Results

The results showed that goat mammary gland expressed NPR-B but not CNP. Activating NPR-B by treatment with CNP had no effect on synthesis of β-casein and lipid in goat MECs. But the expression and secretion of S100A7 in goat MECs were obviously induced by CNP/NPR-B signaling pathway. After treatment with CNP, the cGMP level in goat MECs were significantly up-regulated. Along with the up-regulation of cGMP level, the phosphorylation levels of JNK and its target c-Jun also increased gradually. KT-5823 is a specific inhibitor for PKG. KT5823 remarkably inhibited the phosphorylation of JNK and c-Jun induced by CNP. Correspondingly, KT5823 evidently inhibited the expression and secretion of S100A7 induced by CNP. On the other hand, the expression of NPR-B and S100A7 were up-regulated in mastitis goat mammary gland. But, there were no significant difference in expression of CNP between health and mastitis goat mammary gland tissues. Goat mastitis model was established in vitro using goat MECs treated by lipopolysaccharide (LPS). LPS treatment also could increase the expression of NPR-B and NPR-B.
Conclusions

Goat mammary gland expressed NPR-B, indicating mammary gland was target organ for natriuretic peptide system. Moreover, CNP, through NPR-B/JNK/c-Jun signaling pathway to regulate the expression and secretion of S100A7 in MECs, played an important role in mammary gland innate immune.
Elevated RIF1 Participates in the Epigenetic Abnormalities of Zygotes by Regulating Histone Modifications on MuERV-L in Obese Mice
Authors: Huang Jiliang , Ru Gaizhen , Sun Jiajia , Sun Luying , Li Zhiling 
Published: November 2021

Abstract
Background
Maternal obesity impairs embryonic developmental potential and has significantly increases metabolic risks in offspring. However, the epigenetic transmission mechanism of maternal metabolic abnormalities is still poorly understood.
Methods
We induced an obesity model in female mice by high-fat diet (HFD) feeding. The effects of a HFD on the developmental potential of oocytes and embryos, metabolic phenotype, and epigenetic modifications were investigated. The efficacy of metformin administration was assessed. Finally, the regulatory pathway of epigenetic remodeling during zygotic genome activation (ZGA) was explored.
Results
Maternal HFD significantly impaired glucose tolerance in F0 mice, and increased the risk of metabolic disorders in F1 mice. Maternal HFD also decreased embryonic developmental potential, increased ROS and γH2AX, and reduced the mitochondrial membrane potential (MMP) within oocytes, causing high levels of oxidative stress damage and DNA damage. Starting with this clue, we observed significantly increased RIF1 and shortened telomeres in obese mice. Significant abnormal DNA methylation and histone modification remodeling were observed during ZGA in obese mice, which may be coregulated by RIF1 and the ZGA marker gene MuERV-L. Metformin treatment reduced RIF1, and partially improved ZGA activation status by rescuing epigenetic modification remodeling in oocytes and preimplantation embryos of obese mice. RIF1 knockdown experiments by Trim-Away methods showed that RIF1 degradation altered the H3K4me3 and H3K9me3 enrichment and then triggered the MuERV-L transcriptional activation. Moreover, the ChIP-seq data analysis of RIF1 knockout also showed that RIF1 mediates the transcriptional regulation of MuERV-L by changing the enrichment of H3K4me3 and H3K9me3 rather than DNA methylation.
Conclusion
Elevated RIF1 in oocytes caused by maternal obesity may mediate abnormal embryonic epigenetic remodeling and elevated metabolic risk in offspring by regulating histone modifications on MuERV-L. Metformin treatment can partially rescue the abnormal epigenetic remodeling and prevent epigenetic transmission of maternal metabolic abnormalities.
Biomechanical Evaluation of Reconstruction of Calcar Femorale Structure for Femoral Neck Fractures: A Comparative Finite Element Analysis
Authors: Gao Zhi-Hao , Ma Jian-Xiong , Wang Ying , Lu Bin , Bai Hao-Hao , Sun Lei , Jin Hong-Zhen , Zhang Zi-Jian , Ma Xin-Long 
Published: November 2021

Abstract
The authors have requested that this preprint be removed from Research Square.