bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comparison Of Tolerant And Susceptible Cultivars Revealed The Roles of Circular RNAs In Rice Responding To Salt Stress
Authors: Yin Junliang , Liu Yike , Lu Lin , Zhang Jian , Chen Shaoyu , Wang Baotong 
Published: June 2021

Abstract
As a newly characterized class of noncoding RNAs, circular RNAs (circRNAs) have been identified in many plant species, and play important roles in plant stress responses. However, little is known about how salt stress mediates the expression of circRNAs in rice. In this study, we identified circRNAs from root tissues of salt-susceptible recipient cultivar 93-11 and salt-tolerant introgression line 9L136. A total of 190 circRNAs were identified. Among them, 93 circRNAs were differentially expressed under salt stress in 93-11 (36 up- and 57 down-regulated) and 95 in 9L136 (46 up- and 49 down-regulated). Salt stress significantly decreased the average expression level of circRNAs in 93-11, but circRNA expression levels were slightly increased in 9L136, suggesting that circRNAs had different response patterns in these two cultivars. Function annotation and enrichment analysis indicated that, through cis-regulation and circRNA-miRNA-mRNA network regulation, those induced circRNAs were commonly involved in transcription, signal transduction, ion transportation, and secondary metabolism. Compared to 93-11, salt-induced circRNAs in line 9L136 targeted more stress response genes participating in transcription regulation, ion transportation, and signal transduction, which may contribute to the salt tolerance of 9L136. Summarily, this study revealed the common response of rice circRNAs to salt stress, and the possible circRNA-related salt tolerance mechanisms of 9L136.
Experimental Study of Solar Energy Based Water Purifier (SEBWP) of Single Slope Type by Incorporating N Similar Evacuated Tubular Collectors (ETCs) having Series Connection
Authors: Sharma Sanjeev Kumar , Mallick Ashis , Singh Desh Bandhu , Tiwari Gopal Nath 
Published: June 2021

Abstract
This research paper deals with the experimental investigation of solar energy based water purifier (SEBWP) of single slope type by incorporating N similar evacuated tubular collectors (ETCs) having series connection. Experimental investigation has been done for a year from August 2018 to July 2019. MATLAB has been used for evaluating performance parameters of the system followed by the validation of these results with their experimental values. A fair agreement has been found between theoretical and experimental values. Values of correlation coefficients for condensing glass temperature, water temperature and water yield have been found to be 0.9932, 0.9928 and 0.9951 respectively. Further, energy metrics, productivity, cost of producing one kg of fresh water, exergoeconomic and enviroeconomic parameters have been evaluated. Values of energy payback time, per kg cost of producing fresh water and exergy loss per unit Rs. have been evaluated to be 1.72 years, Rs. 0.95/kg and 0.128 kWh/Rs. respectively.
Antimicrobial Activity of Ceftazidime-Avibactam and Comparators against Levofloxacin-Resistant Escherichia Coli Collected from Four Geographic Regions, 2012–2018
Authors: Stone Gregory 
Published: June 2021

Abstract
Background: Increases in resistance to fluoroquinolones have been correlated with the use of levofloxacin in the treatment of infections caused by Escherichia coli. The analysis presents the in vitro activity of ceftazidime-avibactam and comparator agents against 10,840 levofloxacin-resistant E. coli isolates collected from four geographic regions (Africa/Middle East, Europe, Asia/South Pacific, Latin America) between 2012 and 2018.Methods: Non-duplicate clinical isolates of E. coli were collected from participating centres and shipped to International Health Management Associates, Inc. [IHMA], Schaumburg, IL, USA. Susceptibility testing was performed with frozen broth microdilution panels manufactured by IHMA, according to CLSI guidelines. Levofloxacin-resistance was defined at a minimum inhibitory concentration of ≥2 mg/L. Isolates collected between 2012 and 2015 were tested for extended-spectrum β-lactamase (ESBL) activity by determining susceptibility to cefotaxime, cefotaxime-clavulanate, ceftazidime, and ceftazidime-clavulanate as recommended by CLSI guidelines. Isolates collected between 2016 and 2018 were identified as ESBL-positive by genotype using multiplex polymerase chain reaction assays. Results: A total of 74.8% of levofloxacin-resistant E. coli isolates in the analysis were from three culture sources: urinary tract infections (N = 3,229; 29.8%), skin and skin structure infections (N = 2,564; 23.7%) and intra-abdominal infections (N = 2,313; 21.3%). Susceptibility rates to ceftazidime-avibactam were consistently high in all regions against both ESBL-positive (97.0% in Asia/South Pacific to 99.7% in Africa/Middle East and Latin America) and ESBL-negative isolates (99.4% in Asia/South Pacific to 100% in Latin America). Susceptibility was also high in each region among ESBL-positive and ESBL-negative isolates to colistin (≥98.5%), imipenem (≥96.5%), meropenem (≥96.5%) and tigecycline (≥94.1%). Conclusions: Antimicrobial susceptibility to ceftazidime-avibactam among levofloxacin-resistant E. coli isolates, including ESBL-positive isolates, collected from four geographical regions between 2012 and 2018 was consistently high. Susceptibility to the comparator agents colistin, tigecycline, imipenem and meropenem was also high.
A Focus Group Interview with Health Professionals: Establishing Efficient Transition Care Plan for Older Adult Patients in Korea
Authors: Park Chan Mi , Han Seung Jun , Lee Jae Hyun , Lim Jin , Moon Sung do , Moon Hongran , Lee Seo-Young , Kim Hyeanji , Jang Il-Young , Jung Hee-Won 
Published: June 2021

Abstract
BackgroundEven though transition care can affect functional status and quality of life after acute hospitalization for older adults, less is known on problems associated with discharge planning in Korean acute hospitals. We aimed to investigate barriers and possible solutions on transfer planning of complex older patients in this study.MethodsWe used focus group interview with application of framework analysis. Twelve physicians providing inpatient care from 6 different institutions in Korea participated for the interview. Facilitating questions were extracted from 2 roundtable meetings prior to the main interview. From transcribed verbatim, themes were constructed from corresponding remarks by participants. ResultsWe revealed two main domains of the barrier which included multiple subdomains for each of them. First domain was a patient factor barrier, a composite of misperception of medical provider’s intentions, incomprehension of the healthcare system, and communication failure between the caregivers or decision makers. Second domain, institutional factors included different fee structures across the different levels of care, high barrier to accessing health service in tertiary hospitals or to be referred to, hardship of communication between institutions, and insufficient subacute rehabilitation service across the country. ConclusionsThrough the interview, physicians in the field recognized barriers of smooth transition care process from tertiary level hospitals to community care especially for older adults. Participants emphasized both the patients and hospital sides of adjustment on behaviors, communication, and greater attention for what really matters for the individuals during the transition period.
Development of an environmentally friendly formulation of silk fibroin associated with fatty acid from Astrocaryum murumuru Mart. fat, effective against larvae of the Aedes aegypti vector
Authors: Marinho Victor H. S. , Neves Fernando B. , Jimenez David E. Q. , Oliveira Fabio R. , Santos Abrahão Victor T. L. T. , Ferreira Ricardo M. A. , Souto Raimundo N. P. , Carvalho José C. T. , Yoshioka Sérgio A. , Ferreira Irlon Maciel 
Published: June 2021

Abstract
The Aedes aegypti mosquito is a vector of several diseases, such as dengue, malaria and the Zika virus. Synthetic insecticides such as chlorpyrifos and chlorothalonil have been used for plague control, despite causing damage to the environment and to humans. It is therefore important to study natural active compounds with a low environmental impact. The present paper developed an environmentally friendly formulation of silk fibroin (SF) associated with fatty acid esters [ethyl (FAEE-SF), propyl (FAPE-SF) and butyl (FABE-SF)] from Astrocaryum murumuru Mart. fat, which was effective against Aedes aegypti 3th instar larvae. The FABE-SF nanoemulsion induced a higher mortality rate in the larvae of the A. aegypti after 48 h (LC50 = 18.92 µg / mL). The stabilities of the nanoemulsions were monitored for 21 days, and FABE-SF exhibited greater stability throughout the monitored period, with average particle, zeta and PDI values of around 217 ± 0.85 nm, -25.6 ± 3.24 mV and 0.338 ± 0.01, respectively. This paper reported the first effective action of fatty acid esters from A. murumuru Mart. associated with silk fibroin against A. aegypti. The FABE-SF solution also had a low hemolytic index, suggesting that treatment may be safe for animal and human use.
Robust Quantification of Regional Patterns of Migration in Three-Dimensional Cell Culture Models
Authors: Vong Chun Kiet , Wang Alan , Dragunow Mike , Park Thomas I-H , Shim Vickie 
Published: June 2021

Abstract
Wound healing assays is a common two-dimensional migration model, with the spheroid assay three-dimensional migration model recently emerging as being more representative of in vivo migration behaviours. These models provide insight to the overall migration of cells in response to various factors such as biological, chemotactic and molecular agents. However, currently available analysis techniques for these assays fall short on providing quantifiable means to measure regional migration patterns, , which is essential to allow more robust assessment of drug treatments on cell migration in a chemotactic fashion. Therefore, the aim of this study is to develop a finite element (FE) based pipeline that can objectively quantify regional migration patterns of cells. Here, we report that our FE based approach was able to accurately measure changes in overall migration areas compared to the standard ImageJ method. Furthermore, our regional migration analysis provided accurate and quantitative means to analyse the migration pattern seen in the phantom data and our experimental results, giving us confidence that it can be a robust tool for analysing cell migration patterns.
Simulation of Printer Nozzle for 3D Printing TNT/HMX Based Melt-Cast Explosive
Authors: Zong Huzeng , Cong Qilun , Zhang Tengyue , Hao Yanjun , Xiao Lei , Hao Gazi , Zhang Guangpu , Guo Hu , Hu Yubing , Jiang We 
Published: June 2021

Abstract
Fused deposition modelling (FDM) has been one of the most widely used rapid prototyping (RP) technologies, which has been attracted increasing attentions in the world. However, existing literatures about energetic material flow inside the 3D printer nozzle are sparse. For plunger 3D printer, we summarized the experimental and related literatures, finding that viscosity, temperature, outlet velocity, pressure, and nozzle diameter are the main factors to affect the flow state in the nozzle. Based on the actual printer nozzle structure, in this paper, a finite element model was established by SOLIDWORKS software firstly, meanwhile, the flow channel model of the nozzle was extracted and simplified. Secondly, the factors influencing the printing results were researched and analysed. In the end, numerical simulation on velocity field and temperature field was carried out by FLUENT software. Moreover, the printing test of HMX/TNT was also carried out by using EAM-D-1 3D printer. The printed sample shows that 3D printing is more satisfactory than conventional melt-casting ways to prepare high viscocity and unconventional structure explosives
A Global Bibliometric and Visualized Analysis in The State of Pseudomonas Aeruginosa Efflux Pump Research
Authors: Zhang Ruiqin , Wang Siyang , Ren Jian , feng Jinxin , Li Zhen , Wang Hongxia , Zhi Tingting , Zhu Yuanyuan , wang Yanhui , Yao Zhiqing , duan jinju 
Published: June 2021

Abstract
OBJECTIVE. This study was designed with the goal of surveying the current status of the Pseudomonas aeruginosa efflux pump research field in an effort to explore current and future trends in this clinically relevant research area. METHODS. Bibliometric and visual analyses were used to conduct a global survey of the current status of Pseudomonas aeruginosa efflux pump research. All studies regarding Pseudomonas aeruginosa efflux that were published from 1994 to 2019 were retrieved from the Web of Science database, after which we conducted bibliographic coupling, co-authorship, co-citation, and co-occurrence analyses, and analyzed trends in Pseudomonas aeruginosa efflux pump research publications using the VOS Viewer software. RESULTS. We identified 2583 total publications that were included in this study. The number of relevant studies from around the world was found to be rising over time. The USA was identified as a central player in this research field at a global level, as studies from the USA had the most citations, the highest H-index values, and the greatest total link strength. Three primary research directions were identified through this analysis, including studies of efflux pump resistance mechanisms, transfer mechanisms, and inhibitors. CONCLUSIONS. Our visual analysis provides a quantitative overview for researchers who wish to quickly understand the past and current dynamics of the field of Pseudomonas aeruginosa efflux pump research. Overall, these findings suggest that future studies of efflux pump resistance mechanisms and efflux pump inhibitors may aid in overcoming clinical Pseudomonas aeruginosa resistance.
Geometric Morphology and Population Genomics Provide Insights into the Adaptive Evolution of Apis Cerana in Changbai Mountain
Authors: Nannan Liu , Huamiao Liu , Yan Ju , Xingan Li , Yang Li , Tianjiao Wang , Jinming He , Qingsheng Niu , Xiumei Xing 
Published: June 2021

Abstract
Background: Exploration of adaptive evolution of organisms in response to environmental change will offer us a hint to the evolutionary history of species and the underlying mechanisms of adaptation to local environments, thus guiding future conservation programmes. Before the introduction of Apis mellifera in China, Apis cerana was the only species, which could be reared in captivity to obtain products. Moreover, A. cerana in Changbai Mountain is the only ecotype in such a flora. Result: We investigated the geometric morphological features of A. cerana in Changbai Mountain by analysing 300 wing specimens from 30 populations of A. cerana in 5 geographic regions. A total of 3,859,573 high-quality SNP loci were yielded via the whole-genome resequencing of 130 individuals from 130 A. cerana geographic populations.Conclusion: Corresponding geometric morphology and population genome confirmed the outstanding evolutionary role of the A. cerana population in Changbai Mountain. Genetic differentiation at the subspecies level exists between populations in Changbai Mountain and remaining geographic regions, and a significant reduction in the effective population size and an excessive degree of inbreeding may be responsible for a substantial loss of population genetic diversity. Candidate genes potentially associated with cold environmental adaptations in populations under natural selection were identified, which may represent local adaptations in populations. Our study provided insights into the evolutionary history and adaptive characteristics of A. cerana in Changbai Mountain, as well as the scientific conservation of this population.
Resolvin D1 Inhibits Endothelial Permeability and Mitochondrial Damage Following Cardiac Ischemia–Reperfusion in Diabetic Mice
Authors: Zhang Jialiang , Huang Fangyang , Chen Li , Li Guoyong , Xiao Dan , Lei Wenhua , Zhao Jiahao , Liao Yanbiao , Li Changming , Chen Mao 
Published: June 2021

Abstract
Purpose

Resolvin D1(RvD1), a metabolite derived from docosahexaenoic acid, plays important therapeutic roles in inflammatory diseases. However, the role of RvD1 in diabetic myocardial ischemia/reperfusion (IR) is still unknown.
Methods

Diabetic mice was established by high-fat diet and streptozotocin, RvD1 was pretreated by intraperitoneal injection for 3 days, followed by myocardial IR. To evaluate the effects of RvD1 on chronic cardiac remodeling, RvD1 was administered for another 2 weeks after IR. The effects of RvD1 following myocardial IR injury were measured, including severity of infarct size, regional inflammation, cardiac function, as well as permeability of cultured endothelial monolayer. Mitochondrial reactive oxygen species (mito-ROS) and mitochondrial membrane potential (MMP) were determined by MitoSOX and JC-1.
Results

RvD1 pretreatment significantly reduced infarct size and the content of Evans blue in injured heart, which was associated with decreased endothelial damage. RvD1 also reduced leukocyte density, gene expression of inflammatory cytokines, cardiomyocytes death, and mitochondrial damage compared to control group. At 2 weeks after myocardial IR, RvD1 treatment partially improved cardiac performance, and reduced cardiac fibrosis in diabetic IR. In vitro, RvD1 attenuated endothelial leakage induced by hypoxia-reoxygenation, H2O2, and Lipopolysaccharide (LPS), meanwhile, RvD1 also remarkably protected endothelial cells from H2O2-induced mitochondrial damage as evident from the decreased MMP and increased mito-ROS, which were associated with the preservation of VE-cadherin
Conclusion

RvD1 protects the heart against diabetic IR-induced injuries by attenuating endothelial permeability and mitochondrial damage. Our study also provide insight into a novel underlying mechanism and a new strategy for treating diabetic IR.
The Synergistic Anticancer Effect of Bromodomain Inhibitor OTX015 and Histone Deacetylase 6 Inhibitor WT-161 in Osteosarcoma
Authors: Yu Bo , Liu Lang , Cai Feng , Peng Yuanxiang , Tang Xiaofeng , Zeng Duo , Li Teng , Zhang Feifei , Liang Yiping , Yuan Xuhui , Li Jiayu , Dai Zhengzai , Liao Qi , Lv Xiao-Bin 
Published: June 2021

Abstract
Background: Osteosarcoma (OS) is a tumor with a high malignancy level and a poor prognosis. First-line chemotherapy for OS has not been improved for many decades. Bromodomain and extra terminal domain (BET) and histone deacetylase (HDACs) regulate histone acetylation in tandem, and BET and HDACs have emerged as potential cancer therapeutic targets. Methods: Cell proliferation, migration, invasion, colony formation, and sphere-forming assay were performed with the two inhibitors alone or in combination to evaluate their suppressing effect on malignant properties of OS cells. The apoptosis and cells-cycle profile were measured by flow cytometry. The synergistic inhibitory effect of OTX015/WT-161 on tumor was also examined in a nude mouse xenograft model. Results: The combined therapy of OTX015/WT-161 synergistically inhibited the growth, migration, and invasion and induced apoptosis, resulted in the G1/S arrest of OS cells. Additionally, OTX015/WT-161 inhibits the self-renewal ability of OS stem cells (OSCs) in a synergistic manner. Further mechanistic exploration uncovered that the synergistic downregulation of β-catenin by OTX015-mediated suppression of FZD2 and WT-161-mediated upregulation of PTEN may be responsible for the synergistic effect. Finally, the result of an in vivo assay showed that tumor xenografts were significantly decreased after treatment with OTX015/WT-161 combination compared with OTX015 or WT-161 alone. Conclusion: Our findings in this study demonstrated that OTX015 and WT-161 had a synergistic anti-cancer efficacy against OS, and their combination might be a promising therapeutic strategy for OS.
Distinct mechanisms involving diacylglycerol, ceramides, and inflammation underlie insulin resistance in oxidative and glycolytic muscles from high fat-fed rats
Authors: Jani Shailee , Eira Daniel , Hadday Ishvinder , Bikopoulos George , Mohasses Arta , Pinho Ricardo A. , Ceddia Rolando B. 
Published: June 2021

Abstract
This study investigated whether oxidative and glycolytic skeletal rat muscles respond differently to a high-fat (HF) sucrose-enriched diet with respect to diacylglycerol (DAG) and ceramides accumulation, protein kinase C (PKC) activation, glucose metabolism, and the expression of inflammatory genes. HF diet (8 weeks) suppressed insulin-stimulated glycogen synthesis and glucose oxidation in soleus (Sol), extensor digitorum longus (EDL) and epitrochlearis (Epit) muscles. However, DAG and ceramides levels increased in Sol and EDL, but not in Epit muscles of HF-fed rats. Additionally, membrane-bound PKC-delta and PKC-theta increased in Sol and EDL, whereas in Epit muscles both PKC isoforms were reduced by HF diet. In Epit muscles, HF diet also increased the expression of tumor necrosis factor-α (TNF-α) receptors (CD40 and FAS), toll-like receptor 4 (TLR4), and nuclear factor kappa light polypeptide gene enhancer in B cells (NF-kB), whereas in Sol and EDL muscles the expression of these inflammatory genes remained unchanged upon HF feeding. In conclusion, HF diet caused DAG and ceramides accumulation, PKC activation, and the induction of inflammatory pathways in a fiber type-specific manner. These findings help explain why oxidative and glycolytic muscles similarly develop insulin resistance, despite major differences in their metabolic characteristics and responsiveness to dietary lipid abundance.
Measurement of Entropy and Quantum Coherence Properties of Two Type-I Entangled Photonic Qubits
Authors: motazedifard ali , Madani Seyed Ahmad , Vayaghan Nader Sobhkhiz 
Published: June 2021

Abstract
Using the type-I SPDC process in BBO nonlinear crystal (NLC), we generate a polarization-entangled state near to the maximally-entangled Bell-state with high-visibility (high-brightness) 98.50 ±1.33 % (87.71 ± 4.45 %) for HV (DA) basis. We calculate the CHSH version of the Bell inequality, as a nonlocal realism test, and find a strong violation from the classical physics or any hidden variable theory (HVT), S= 2.71±0.10. Via measuring the coincidence count (CC) rate in the SPDC process, we obtain the quantum efficiency of single-photon detectors (SPDs) around (25.5 ±3.4)% , which is in good agreement to their manufacturer company. As expected, we verify the linear dependency of the CC rate vs. pump power of input CW-laser, which may yield to find the effective second-order susceptibility crystal. Using the theory of the measurement of qubits, includes a tomographic reconstruction of quantum states due to the linear set of 16 polarization-measurement, together with a maximum-likelihood-technique (MLT), which is based on the numerical optimization, we calculate the physical non-negative definite density matrices, which implies on the non-separability and entanglement of prepared state. By having the maximum likelihood density operator, we calculate precisely the entanglement measures such as Concurrence, entanglement of formation, tangle, logarithmic negativity, and different entanglement entropies such as linear entropy, Von-Neumann entropy, and Renyi 2-entropy. Finally, this high-brightness and low-rate entangled photons source can be used for short-range quantum measurements in the Lab.