bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Multimodal Mobile Sleep Intervention for Young Adults Engaged in Risky Drinking: Protocol for a Randomized Controlled Trial
Authors: Fucito Lisa M , Ash Garrett I , DeMartini Kelly S , Pittman Brian , Barnett Nancy P , Li Chiang-Shan R , Redeker Nancy S , O'Malley Stephanie S 
Published: January 2021

Background
This paper describes the research protocol for a randomized controlled trial of a multimodal mobile sleep intervention for heavy-drinking young adults. Young adults report the highest rates of heavy, risky alcohol consumption and are a priority population for alcohol prevention and intervention efforts. Alcohol strategies that leverage other health concerns and use technology may offer an innovative solution. Poor sleep is common among young adults and is a risk factor for developing an alcohol use disorder. Moreover, young adults are interested in information to help them sleep better, and behavioral sleep interventions address alcohol use as a standard practice.

Objective
The primary aim of this study is to assess the effectiveness of a 2-week multimodal mobile sleep intervention for reducing drinks consumed per week among heavy-drinking young adults. We will explore the effects on alcohol-related consequences, assessing quantitative and qualitative sleep characteristics as secondary aims. The study’s goals are to identify the optimal combination of sleep intervention components for improving drinking outcomes, the feasibility and acceptability of these components, and the potential mechanisms by which these components may promote alcohol behavior change.

Methods
Young adults (aged 18-25 years) who report recent heavy drinking will be randomly assigned to one of three conditions: mobile sleep hygiene advice (n=30), mobile sleep hygiene advice and sleep and alcohol diary self-monitoring (n=30), or mobile sleep hygiene advice, sleep and alcohol diary self-monitoring, and sleep and alcohol data feedback (n=60). For the feedback component, participants will complete two web-based sessions with a health coach during which they will receive summaries of their sleep and alcohol data, and the potential association between them along with brief advice tailored to their data. All participants will wear sleep and alcohol biosensors daily for 2 weeks for objective assessments of these outcomes.

Results
The study was funded by the National Institutes of Health in May 2018. Recruitment began in December 2018 and will be concluded in Spring 2021. As of February 4, 2021, we have enrolled 110 participants.

Conclusions
Ultimately, this research could result in an efficacious, low-cost intervention with broad population reach through the use of technology. In addition, this intervention may substantially impact public health by reducing alcohol use disorder risk at a crucial developmental stage.

Trial Registration
ClinicalTrials.gov NCT03658954; https://clinicaltrials.gov/ct2/show/NCT03658954

International Registered Report Identifier (IRRID)
DERR1-10.2196/26557
Bioinformatic Approaches for Identification of Potential Repurposable Drugs in COVID-19
Authors: Mandal Manisha , Mandal Shyamapada 
Published: January 2021

Introduction: Repurposing existing drugs approved for other conditions is crucial to identifying specific therapeutics against SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2) causing COVID-19 (coronavirus disease 2019) pandemic. Towards this attempt, it is important to understand how this virus hijacks the host system during the course of infection and determine potential virus- and host-targeted inhibitors.
Methods: This study elucidates the underlying virus-host interaction based on differentially expressed gene profiling, functional enrichment and pathway analysis, protein-protein and protein-drug interactions utilizing the information on transcriptional response to SARS-CoV-2 infection from GSE147507 dataset containing COVID-19 case relative to healthy control and infected cell culture compared to uninfected one.
Results: Low IFN signaling, chemokines level elevation, and proinflammatory cytokines release were observed markedly. We identified MYC-rapamycin and ABCG2-rapamycin interactions, and unique gene signatures in case (regulation of protein modification and MAPK signaling) as well as in cell (metabolic dysregulation and interferon signaling) different from known COVID-19 genes.
Conclusion: Among a plethora of repurposable drugs those appearing here with unique gene signatures might be helpful in COVID-19
Keywords: COVID-19, SARS-CoV-2, GSE 147507 dataset, protein-protein interaction, gene-drug interaction, repurposable drugs.
Bioadhesive polymers, permeation enhancers and types of dosage forms for buccal drug delivery
Authors: Samanthula Kumara Swamy , Satla Shobha Rani , Bairi Agaiah Goud 
Published: January 2021

The buccal delivery is defined as the drug administration through the mucosal membranes lining the cheeks (buccal mucosa). The main impediment to the use of many hydrophilic macromolecular drugs as potential therapeutic agents is their inadequate and erratic oral absorption. Based on our current understanding of biochemical and physiological aspects of absorption and metabolism of many biotechnologically produced drugs, they cannot be delivered effectively through the conventional oral route. Because after oral administration many drugs are subjected to pre-systemic clearance extensive in the liver, which often leads to a lack of significant correlation between membrane permeability, absorption and bioavailability. Difficulties associated with the parenteral delivery and poor oral bioavailability provided the impetus for exploring alternative routes for the delivery of such drugs. This review covers the advantages, disadvantages of buccal delivery, drug and excipient selection especially bioadhesive polymers and permeation enhancers, and further a list of drugs developed as various dosage forms for buccal route of administration.
Keywords: Buccal delivery, bioadhesive/mucoadhesive, permeation enhancer, dosage forms.
Formulation and Evaluation of Liposome by Thin Film Hydration Method
Authors: Umbarkar Mahesh , Thakare Swapnil , Surushe Tanaji , Giri Amol , Chopade Vaibhav 
Published: January 2021

Liposomes are the most advance formulation for targeting and controlled drug delivery system. These liposomes are generally administered by intra-venous route. In this work the liposome was prepared by using thin film hydration method. The formulated liposome is evaluated or characterised by using zeta sizer, Encapsulation efficiency, Entrapment efficiency, In vitro drug release. Main things are drug which are used for formulation of liposome was Diclofenac sodium, it having anti-inflammatory and anti-pyretic effect. The Diclofenac sodium having several adverse effects, such as depression of renal function, Liver failure for repeated administration, Local mucosal irritation, gastritis. To avoid this adverse effect Diclofenac sodium are incorporate in liposomal formulation. By formulating liposomal formulation, the bioavailability of Diclofenac sodium increase. In conventional dosage form bioavailability of diclofenac sodium is 50℅. But in liposomal formulation bioavailability of this drug increase. The final result includes that diclofenac liposome formulation shows more sustained and prolong anti-inflammatory activity.
Keywords: Diclofenac sodium, Liposome, Anti-inflammatory activity.
Green-Blue Spaces and Mental Health: A Longitudinal Data Linkage Study
Authors: Thompson Daniel A , Nieuwenhuijsen Mark , White James , Lovell Rebecca , White Mathew , Lyons Ronan A , Stratton Gareth , Akbari Ashley , Geary Rebecca , Wheeler Ben , Watkins Alan , Fry Richard , Rowney Francis , Mizen Amy , Rodgers Sarah E 
Published: January 2021

IntroductionA growing evidence base indicates health benefits are associated with access to green-blue spaces (GBS), such as beaches and parks. However, few studies have examined associations with changes in access to GBS over time.
Objectives and ApproachWe have linked cross-sector data collected within Wales, United Kingdom, quarterly from 2008 to 2019, to examine the impact of GBS access on individual-level well-being and common mental health disorders (CMD). We created a longitudinal dataset of GBS access metrics, derived from satellite and administrative data sources, for 1.4 million homes in Wales. These household-level metrics were linked to individuals using the Welsh Demographic Service Dataset within the Secure Anonymised Information Linkage (SAIL) Databank. Linkage to Welsh Longitudinal General Practice data within SAIL enabled us to identify individual-level CMD over time. We also linked individual-level self-reported GBS use and well-being data from the National Survey for Wales (NSW) to routine data for cross-sectional survey participants.
ResultsWe created a longitudinal cohort panel capturing all 2.84 million adults aged 16+ living in Wales between 2008 and 2019 and with a general practitioner (GP) registration. Individual-level health data and household-level environmental metrics were linked for each quarter an individual is in the study. Household addresses were linked to 97% of the cohort, creating 110+ million rows of anonymously linked cross-sector data. The cohort provides an average follow-up period of 8 years, during which 565,168 (20%) adults received at least one CMD diagnosis or symptom.
Conclusion / ImplicationsThis example of multi-sectoral data linkage across multiple environmental and administrative data sources has created a rich data source, which we will use toquantify the impact of changes in GBS access on individual–level CMD and well-being. This evidence will inform policy in the areas of health, planning and the environment.
PENAMBAHAN BEBERAPA JENIS BAHAN NUTRISI PADA MEDIA PERBANYAKAN UNTUK MENINGKATKAN VIRULENSI BEAUVERIA BASSIANA TERHADAP HAMA WALANG SANGIT
Authors: Nuryanti Ni Siluh Putu , Wibowo Lestari , Azis Abdul 
Published: January 2021

A research was conducted to increase the virulence of Beauvaria bassiana to the rice bug, L. acuta, by adding four different substances (materials) to the rice media to grow the fungus. The research was conducted from April to August 2011 at Polinela Laboratory, Bandar Lampung. To do this a set of experimental unit consisting of five treatments and five replications were arranged in completely randomized design. The four treatments were media made up of rice (rice based media) each given or added with four different materials such as rice skin powder, corn sugar, grasshopper powder and chitosan powder. The result showed that B. bassiana grown on rice media added with grasshopper powder or rice skin powder were able of causing mortality to the L. acuta as high as 78% and 71%, respectively, meaning that both mortalities were much higher than that of other replication. The virulence values of Beauveria bassiana grown on those both powder were also higher than that of three others.
KOMPATIBILITAS CENDAWAN ENTOMOPATOGEN BEAUVERIA BASSIANA (BALS) VUILL (DEUTEROMYCOTINA: HYPHOMYCETES) DENGAN MINYAK SERAI WANGI
Authors: Trizelia Trizelia , Rusli Rusdi 
Published: January 2021

Entomopathogenic fungi such as Beauveria bassiana are important natural control agents of many insects and can be potentially used as a bioinsecticide against several pests. Other potential source of bioinsecticide is certain plants such as fragrant lemongrass oil. The in vitro compatibility of the entomopathogenic fungus B. bassiana and fragrant lemongrass oil was evaluated. Fragrant lemongrass oil was tested in three different concentrations (0.1, 0.3 and 0.5%). Fragrant lemongrass oil was diluted in the steril SDAY medium at the different concentrations. Effects of these concentrations on conidia germination, colony growth and sporulation were compared. Fragrant lemongrass oil affected conidial germination, colony growth and sporulation of B. bassiana. Fragrant lemongrass oil was not compatible with the entomopathogenic fungus B. bassiana.
PATOGENISITAS ISOLAT BEAUVERIA BASSIANA DAN Metarhizium anisopliae ASAL TANAH LEBAK DAN PASANG SURUT SUMATERA SELATAN UNTUK AGENS HAYATI Scirpophaga incertulas
Authors: Thalib Rosdah , Fernando Redi , Khodijah Khodijah , Meidalima Dewi , Herlinda Siti 
Published: January 2021

Pathogeicity of Beauveria bassiana and Metarhizium anisopliae Isolates from Fresh Swamp and Tidal Lowland, South Sumatra for Scirpophaga incertulas Biological Agents. The objectives of the research weret o explore and to determine the pathogenicity of entomopathogenic fungi againts the larvae of Scirpophaga incertulas, and to measure conidial viability and density of the fungi. The method for fungi exploration used larvae of Tenebrio molitor baiting submerged in the soil. The soil was taken from fresh swampand tidal lowland rice in South Sumatra. From the exploration study, we found two species of entomopathogenic fungi: Beauveria bassiana and Metarhizium anisopliae. Mortality of S. incertulas larvae that had been treated topically with fungal conidia (1x106 conidia ml-1) varied among the isolates. The highest mortality (98.33%) caused by BPlus isolate of B. Bassiana and the lowest by MtmIn isolate of M. anisopliae (57.50%) and BTmTr isolate of B. bassiana (57.50%). The fungal colonies grew fast from the second day up to the fourth day after incubation but the growth became slow after the fifth day. The highest conidial density was resulted by BPcMs of B. bassiana isolate (63.33x106 conidia ml-1) but this density was not significantly different from that of the BPlus of B. bassiana isolate (63.11x106 conidia ml-1). The lowest conidial density found in BTmTr of B. bassiana isolate (20.97x106 conidia ml-1) . The isolate B. bassiana was more effective than M. anisopliae againt the larvae of S.incertulas.
TOLERANSI TANAMAN PENEDUH POLYALTHIA LONGIFOLIA DAN PTEROCARPUS INDICUS TERHADAP Ganoderma sp.
Authors: Widyastuti Siti Muslimah , Harjono Harjono , Riastiwi I 
Published: January 2021

Susceptibility of Urban Trees Polyalthia longifolia and Pterocarpus indicus to Infection of the red root rot fungus Ganoderma sp. Urban trees on the Gadjah Mada University (UGM) area play an important role in increasing environmental qualities as well as in supporting the teaching and learning processes. However, red root rot disease caused by Basidiomycete Ganoderma sp. has severely infected some existing urban trees. This experiment was aimed to determine the susceptibility of Polyalthia longifolia (glodokan) and Pterocarpus indicus (angsana) to the infection of Ganoderma sp. Identification of infected trees was performed in UGM area. Further steps were carried out to achieve those objectives : (1) isolation of Ganoderma spp. and testing of Koch’s postulate and (2) examination of the susceptibility of P. longifolia and P. indicus to infection of Ganoderma sp. The susceptibility test of P. longifolia and P. indicus to Ganoderma sp. indicated that P. longifolia was more resistant to fungal pathogen infection than that of P. indicus. Based on this experiment, it can be concluded that P. longifolia is a species that is more suitable than P. indicus. P. longifolia should be planted on the areas that have been infested with inocula of Ganoderma sp..
PENINGKATAN KETAHANAN TANAMAN PISANG BARANGAN TERHADAP BLOOD DISEASE BACTERIUM (BDB) DENGAN APLIKASI FUNGI MIKORIZA ARBUSKULAR INDIGENUS
Authors: Suswati Suswati , Najril Nasir , Azwana Azwana 
Published: January 2021

Increase resistence of barangan banana blood disease bacterium using Indigenus arbuscular mycorhizae fungi. Blood disease caused by Blood disease bacterium (BDB) is the major cause of production loss of banana in Indonesia. There is currently a lack of information about the application of indigenous Arbuscular mycorrhizal fungi (AMF) to increase the Barangan banana resistance to BDB. A greenhouse experiment was conducted to evaluate the increase resistance Barangan banana to 3 (three) types of AMF (Glomus type-1; Acaulospora type-4 and Glomus fasciculatum) and control (without AMF) with 3 replicates. Barangan banana plantlet were inoculated with 50 g fresh AMF inoculants and at 14 days after acclimating have transplanted to soil contaminated BDB. Observation on the Barangan banana seedlings was done after 5-60 days after application included percentage disease, severity disease, incubation periode, BDB population in rhizosfer and AMF percentage root colonization. The result indicated that AMF increased Barangan banana resistance to BDB. Glomus type-1 and G. fasciculatum increased banana resistance by 100% while Acaulospora type-4 takes 66.67% and control none. The disease severity with Acaulospora tipe-4 is 6% lower than controls (32.6%). The highest suppression level was found in 2 isolates of Glomus application was 100% and was followed Acaulospora type-4 is 81.59%. The low intensity of on FMA indigenous treatment applications related to the incubation period and BDB density in rhizosphere of plant roots. The incubation period in mychorized plants has longer 30 dap than controls (10 dap).
PARAMETER NERACA HAYATI DAN PERTUMBUHAN POPULASI KUTU PUTIH Phenacoccus manihoti Matile-Ferrero (HEMIPTERA: PSEUDOCOCCIDAE) PADA DUA VARIETAS UBI KAYU
Authors: Wardani Nila , Rauf Aunu , Winasa Wayan , Santoso Sugeng 
Published: January 2021

ABSTRACTThe life history and population growth parameters of mealybug Phenacoccus manihoti Matile-Ferrero (Hemiptera: Pseudococcidae) on two cassava varieties. The development, reproduction, and population growth parameters of the cassava mealybug, Phenacoccus manihoti Matile-Ferrero (Hemiptera: Pseudococcidae) on two cassava varieties were studied in laboratory. The varieties tested were UJ-5 with high cyanide content (>100 mg per kg) dan Adira-1 with low cyanide content (27.5 mg per kg). Our research revealed that P. manihoti performances were highly affected by cassava varieties. Incubation period of eggs of P. manihoti were 7.93 ± 0.09 and 8.33 ± 0.11 days, nymphal development periode 12.32±0.13 and 15.67 ± 0.13 days, respectively on UJ-5 and Adira-1. Fecundity averaged 386.37 ± 5.83 on UJ-5 and 318.67±2.81 eggs on Adira-1. Intrinsic rate of increase (rm) were 0.258 ± 0.001 on UJ-5 and 0.220 ± 0.001 on Adira-1. Mean generation time (T) on UJ-5 and Adira-1 were 22.795 ± 0.050 and 25.532 ± 0.047 days, repectively. Our findings showed that variety UJ-5 was more suitable for development and population growth of the cassava mealybug.
DIAGNOSIS PENYAKIT MOSAIK (SOYBEAN MOSAIC VIRUS) TERBAWA BENIH KEDELAI
Authors: Andayanie Wuye Ria 
Published: January 2021

Soybean mosaic disease is wide spread throughout soybean-growing countries. Incidence of this disease in East Java is caused by Soybean mosaic virus (SMV) and cowpea mild mottle virus (CMMV). This aim of study was to find the etiology of disease at 14-28 days after planting (dap) on soybean. Research was done by observing visual symptoms. Visual symptoms confirmed by infectivity test, serology assay,electron microscopy (EM) and molecular detection. Results from experiment indicated that soybean plants (14-28 dap) with symptom could be detected in infectivity test. Mechanical inoculation with symptomatic leaf extracts produced local lessions on Chenopodium amaranticolor. Positive results were obtained for Madiun, Ngawi, and Magetan samples when tested against antiserum of SMV in serological assay, however Ponorogo samples were not detected by serological assay. Electron microscopy was also done for the selected sample to confirm the result of positive results. In EM observations, characteristic filamentous particles with modal length close to 900 nm were observed in samples infected with SMV. We detected an array of amplification products of expected size 1385 bp fragment of cylindrical inclusion gene from Madiun, Ngawi, and Magetan isolates in former fields, but not detected in Ponorogo isolate. This result showed the existence of SMV of soybean seed transmission at 14-28 dap in Madiun, Magetan and Ngawi.
UJI KETAHANAN GALUR-GALUR KENTANG TRANSGENIK HASIL TRANSFORMASI DENGAN GEN RB TERHADAP PENYAKIT HAWAR DAUN (PHYTOPHTHORA INFESTANS) DI KP PASIRSARONGGE, CIANJUR
Authors: Listanto Edy , Herman M. , Sofiari Eri 
Published: January 2021

Resistance test strains of transgenic potatoes transformed with RB gene to late blight (Phytophthora infestan) in KP Pasirsarongge, Cianjur. Potato late blight caused by Phytophthora infestans (P. infestans) (Mönt.) de Barry continues to be one of the most important crop diseases of all time. Genetic engineering of potato using RB gene for resistant plant to this disease is the most effective and environmental friendly to prevent widespread of late blight. This research aims to perform resistance of transgenic potato lines containing RB gene to lateblight (P. infestans) in Pasirsarongge, Cianjur field trial station. The first generation of transgenic lines were planted on polybag containing soil:manures using randomized complete block design. Tested plant inoculation was done naturaly from inoculum source from border row (Granola) that has been planted at one month before. The symptom was observed at one month after planting and damage scoring was done every three days for five times. Twenty two transgenic lines of tested plant showed various resistance respond to late blight (P. infestans) attack. Three transgenic lines showed highly resistance to late blight (P. infestans) were lines 11, 24, and 25, one transgenic line has resistant level was line 6.
DEVELOPMENT OF SIMPLE, EASY AND INEXPENSIVE ONLINE SYSTEM FOR LIST OF PROPOSAL ASSESSMENT CREDIT SCORE OF WIDYAISWARA
Authors: Satria Wirawan Sri Mahendra 
Published: January 2021

The current process of proposing and calculating credit number for Widyaiswara is felt to require an exceedingly difficult effort. Many costs must be incurred, especially to hold office stationery such as printing equipment, paper, ink, binder clips, and others. The next issue is how to provide a relatively large number of proposal files and a place for verification and evaluation. After the research is finished, the problem arises again when it will destroy the documents that have been examined. This condition causes waste generation which is not environmentally friendly. An alternative solution for this is to use an online system of calculating credit numbers. However, the development and use of online system applications require considerable development, maintenance, and development costs. Based on research conducted using Microsoft Excel software combined with several software that provides unpaid facilities, a credit score calculation system application can be built for Widyaiswara that is simple, easy, and inexpensive. The results of trials conducted on the calculation of Widyaiswara BPSDM credit figures in DKI Jakarta Province gave very satisfying results, especially on increasing the speed of time and accuracy of proposals and assessments.
PENERAPAN MODEL PEMBELAJARAN PROJECT BASED LEARNING (PJBL) UNTUK MENINGKATKAN HASIL BELAJAR IPA PADA MATERI BIOTEKNOLOGI DAN PRODUKSI PANGAN DALAM PEMBELAJARAN DARING
Authors: Ramadhani Fakhri 
Published: January 2021

Tujuan penelitian ini adalah untuk mengetahui peningkatan hasil belajar IPA setelah penerapan model pembelajaran Project Based Learning (PjBL) untuk meningkatkan hasil belajar IPA pada materi bioteknologi dan produksi pangan dalam pembelajaran daring di Kelas IX-1 SMP Negeri 2 Binjai Kab. Langkat T.P. 2019/2020. Penelitian ini dilaksanakan dalam kelas meliputi kegiatan pelaksanaan tindakan kelas (PTK) berupa kegiatan refleksi awal dan melakukan observasi untuk mengidentifikasi permasalahan yang terjadi di kelas, perencanaan pembelajaran, pelaksanaan tindakan, observasi dan refleksi. Adapun temuan penelitian PTK ini adalah: 1) Pada siklus I sebanyak 15 orang atau sekitar 62,5% dan sebanyak 9 orang tidak tuntas atau sekitar 37,5%; 2) Pada siklus II sebanyak 22 orang tuntas atau sekitar 91,7% dan sebanyak 2 orang tidak tuntas atau sekitar 8,3%. Dapat disimpulkan bahwa penerapan model pembelajaran projek based learning (PjBL) dapat meningkatkan hasil belajar siswa di SMP Negeri 2 Binjai Kab. Langkat T.P. 2019/2020 dengan pembelajaran daring pada masa pandemi Covid-19, pada materi bioteknologi produksi pangan.