bluuebunny/crossref_metadata_2025_split huggingface dataset:
Flow cytometric myeloma measurable residual disease testing in the era of targeted therapies
Authors: Ng David P. 
Published: July 2021

AbstractTherapies in myeloma are rapidly advancing with a host of new targeted therapies coming to market. While these drugs offer significant survival benefits and better side‐effect profiles compared with conventional chemotherapeutics, they raise significant difficulties in monitoring post‐therapy disease status by flow cytometry due to assay interference and/or selection of phenotypically different sub‐clones. The principal culprit, anti‐CD38 monoclonal antibodies, limits the ability to detect plasma cells based on classical CD38/CD45 gating. Other markers, such as CD138, are known to be suboptimal by flow cytometry. Various techniques have been proposed to overcome this problem. The most promising of these techniques has been the marker VS38c, a monoclonal antibody targeting an endoplasmic reticulum protein which has shown high sensitivity for plasma cells. Alternative techniques for gating plasma cells, while variably effective in the near term are already the subject of several targeted therapies rendering their usefulness limited in the longer term. Likewise, future targets of these therapies may render present aberrancy markers ineffective in MRD testing. These therapies pose challenges that must be overcome with new markers and novel panels in order for flow cytometric MRD testing to remain relevant.
A systematic review of empirical research on learning with3Dprinting technology
Authors: Novak Elena , Brannon Megan , Librea‐Carden Mila Rosa , Haas Amy L. 
Published: July 2021

AbstractAlthough 3D printing (3DP) technology has become an increasingly popular educational tool in recent years, very little is known about the learning benefits of this technology. This systematic literature review synthesized empirical research on learning with 3DP in various educational settings, focusing on publication and study participants' characteristics, curriculum areas, research methodologies, instructional approaches, educational outcomes and benefits. A comprehensive survey of published and unpublished studies identified 78 empirical studies that met the inclusion criteria. In addition to positive effects on learning, 3DP facilitated innovative curriculum development and created opportunities for cross‐disciplinary research. The findings revealed five major trends in 3DP learning: (1) prepare a new generation of engineers, (2) democratize additive manufacturing technology and production, (3) support learning using low‐cost 3D printed learning aids, (4) produce assistive technologies, and (5) promote creativity and innovation. The review identifies critical gaps in the literature and offers suggestions for future research.
Peripheral blood transcriptome heterogeneity and prognostic potential in lung cancer revealed by RNA‐Seq
Authors: Zhang Qi , Kuang Manchao , An Haiyin , Zhang Yajing , Zhang Kai , Feng Lin , Zhang Lei , Cheng Shujun 
Published: July 2021

AbstractUnderstanding of the complex interaction between the peripheral immune system and lung cancer (LC) remains incomplete, limiting patient benefit. Here, we aimed to characterize the host peripheral immune response to LC and investigate its potential prognostic value. Bulk RNA‐sequencing data of peripheral blood leucocytes (PBLs) from healthy volunteers and LC patients (n = 142) were analysed for characterization of host systemic immunity in LC. We observed broad blood transcriptome perturbations in LC patients that were heterogeneous, as two new subtypes were established independent of histology. Functionally, the heterogeneity between the two subtypes included dysregulation of diverse biological processes, such as the cell cycle, blood coagulation and inflammatory signalling pathways, together with the abundance and activity of blood cells, particularly lymphocytes and neutrophils, ultimately manifesting as differences in antitumour immune status. Based on these findings, a prognostic model composed of ten genes dysregulated in one LC subtype with relatively poor immune status was developed and validated in a Gene Expression Omnibus (GEO) data set (n = 108), helping to generate a prognostic nomogram. Collectively, our study provides novel and comprehensive insight into the heterogeneity of the host peripheral immune response to LC. The expression heterogeneity–based predictive model may help guide prognostic management for LC patients.
COVID‐19 Transmission during Transportation of 1st to 12th Grade Students: Experience of an Independent School in Virginia
Authors: Ramirez Dana W.E. , Klinkhammer Martin D. , Rowland Leah C. 
Published: July 2021

AbstractBackgroundIn‐school transmission of COVID‐19 among K‐12 students is low when mitigation layers are used, but the risk of acquiring COVID‐19 during school bus transportation is not well defined. Given the operational limitations of many school districts, more data is needed to determine what mitigation is required to keep COVID‐19 transmission low during bus transport.MethodsAn independent school in Virginia monitored 1154 students in grades 1 to 12 with asymptomatic PCR testing every 2 weeks from August 24, 2020 to March 19, 2021, during the highest community transmission. Fifteen buses served 462 students while operating at near capacity of 2 students in every seat, using a physical distancing minimum of 2.5 ft, universal masking, and simple ventilation techniques.ResultsA total of 39 individuals were present on buses during their COVID‐19 infectious period, which resulted in the quarantine of 52 students. Universal testing and contact tracing revealed no transmission linked to bus transportation.ConclusionsThis study demonstrates a model for the safe operation of school buses while near capacity. COVID‐19 transmission can be low during student transport when employing mitigation including simple ventilation, and universal masking, at minimal physical distances and during the highest community transmission.
Adaptive and nonadaptive causes of heterogeneity in genetic differentiation across the Mimulus guttatus genome
Authors: Colicchio Jack M. , Hamm Lauren N. , Verdonk Hannah E. , Kooyers Nicholas J. , Blackman Benjamin K. 
Published: July 2021

AbstractGenetic diversity becomes structured among populations over time due to genetic drift and divergent selection. Although population structure is often treated as a uniform underlying factor, recent resequencing studies of wild populations have demonstrated that diversity in many regions of the genome may be structured quite dissimilar to the genome‐wide pattern. Here, we explored the adaptive and nonadaptive causes of such genomic heterogeneity using population‐level, whole genome resequencing data obtained from annual Mimulus guttatus individuals collected across a rugged environment landscape. We found substantial variation in how genetic differentiation is structured both within and between chromosomes, although, in contrast to other studies, known inversion polymorphisms appear to serve only minor roles in this heterogeneity. In addition, much of the genome can be clustered into eight among‐population genetic differentiation patterns, but only two of these clusters are particularly consistent with patterns of isolation by distance. By performing genotype‐environment association analysis, we also identified genomic intervals where local adaptation to specific climate factors has accentuated genetic differentiation among populations, and candidate genes in these windows indicate climate adaptation may proceed through changes affecting specialized metabolism, drought resistance, and development. Finally, by integrating our findings with previous studies, we show that multiple aspects of plant reproductive biology may be common targets of balancing selection and that variants historically involved in climate adaptation among populations have probably also fuelled rapid adaptation to microgeographic environmental variation within sites.
Broadening the roles of UDP‐glycosyltransferases in auxin homeostasis and plant development
Authors: Mateo‐Bonmatí Eduardo , Casanova‐Sáez Rubén , Šimura Jan , Ljung Karin 
Published: July 2021

Summary

The levels of the important plant growth regulator indole‐3‐acetic acid (IAA) are tightly controlled within plant tissues to spatiotemporally orchestrate concentration gradients that drive plant growth and development. Metabolic inactivation of bioactive IAA is known to participate in the modulation of IAA maxima and minima.

IAA can be irreversibly inactivated by oxidation and conjugation to aspartate and glutamate. Usually overlooked because of its reversible nature, the most abundant inactive IAA form is the IAA‐glucose (IAA‐glc) conjugate.

Glycosylation of IAA in Arabidopsis thaliana is reported to be carried out by UDP‐glycosyltransferase 84B1 (UGT84B1), while UGT74D1 has been implicated in the glycosylation of the irreversibly formed IAA catabolite oxIAA.

Here we demonstrated that both UGT84B1 and UGT74D1 modulate IAA levels throughout plant development by dual IAA and oxIAA glycosylation. Moreover, we identified a novel UGT subfamily whose members redundantly mediate the glycosylation of oxIAA and modulate skotomorphogenic growth.
SUMOylation of different targets fine‐tunes phytochrome signaling
Authors: Péter Csaba , Nagy Ferenc , Viczián András 
Published: July 2021

SummaryPlants monitor their surrounding ambient light environment by specialized photoreceptor proteins. Among them, phytochromes monitor red and far‐red light. These molecules perceive photons, undergo a conformational change, and regulate diverse light signaling pathways, resulting in the mediation of key developmental and growth responses throughout the whole life of plants. Posttranslational modifications of the photoreceptors and their signaling partners may modify their function. For example, the regulatory role of phosphorylation has been investigated for decades by using different methodological approaches. In the past few years, a set of studies revealed that ubiquitin‐like short protein molecules, called small ubiquitin‐like modifiers (SUMOs) are attached reversibly to different members of phytochrome signaling pathways, including phytochrome B, the dominant receptor of red light signaling. Furthermore, SUMO attachment modifies the action of the target proteins, leading to altered light signaling and photomorphogenesis. This review summarizes recent results regarding SUMOylation of various target proteins, the regulation of their SUMOylation level, and the physiological consequences of SUMO attachment. Potential future research directions are also discussed.
The WOMBS Framework: A review and new theoretical model for investigating pregnancy‐related weight stigma and its intergenerational implications
Authors: Incollingo Rodriguez Angela C. , Nagpal Taniya S. 
Published: July 2021

SummaryAs the growing weight stigma literature has developed, one critically relevant and vulnerable population has received little consideration—pregnant and postpartum women. Because weight fluctuations are inherent to this life phase, and rates of prepregnancy overweight and obesity are already high, this gap is problematic. More recently, however, there has been a rising interest in pregnancy‐related weight stigma and its consequences. This paper therefore sought to (a) review the emerging research on pregnancy‐related weight stigma phenomenology and (b) integrate this existing evidence to present a novel theoretical framework for studying pregnancy‐related weight stigma. The Weight gain, Obesity, Maternal‐child Biobehavioral pathways, and Stigma (WOMBS) Framework proposes psychophysiological mechanisms linking pregnancy‐related weight stigmatization to increased risk of weight gain and, in turn, downstream childhood obesity risk. This WOMBS Framework highlights pregnant and postpartum women as a theoretically unique at‐risk population for whom this social stigma engages maternal physiology and transfers obesity risk to the child via social and physiological mechanisms. The WOMBS Framework provides a novel and useful tool to guide the emerging pregnancy‐related weight stigma research and, ultimately, support stigma‐reduction efforts in this critical context.
Wenckebach cycle length: A novel predictor for AV block in AVNRT patients treated with ablation
Authors: Chatzidou Sofia , Kontogiannis Christos , Georgiopoulos Georgios , Kosmopoulos Marinos , Pateras Konstantinos , Spartalis Michael , Stamatelopoulos Kimon , Rokas Stelios 
Published: July 2021

AbstractBackgroundRadiofrequency catheter ablation remains the most effective management option for atrioventricular nodal reentry tachycardia (AVNRT). The risk of atrioventricular (AV) block requiring permanent pacemaker is substantial, but, currently, a reliable method to predict this complication is lacking.MethodsThe electrophysiologic studies (EPS) and baseline characteristics of patients who underwent catheter ablation for the treatment of AVNRT were retrospectively analyzed to investigate predisposing factors for AV block after treatment. Patients were followed for AV block at one month and one year after hospital discharge.ResultsAmong 784 patients treated with catheter ablation for AVNRT between 1999 to 2019, 15 developed AV block. Patients with AV block were older (p = .001). Among the recorded EPS parameters, patients with AV block had significantly higher Atrial His interval (120 vs. 110 ms, p = .049), Wenckebach cycle length (WCL) (400 vs. 353 ms, p < .001) and tachycardia CL (400 vs. 387 ms, P = .01) during the ablation compared to their peers without AV block. Additionally, only WCL (OR = 1.1, 95% CI 1.02‐1.19, p = .017) remained significant after adjustment for age, gender, ERP, AH interval, and HR. This association was confirmed by comparing patients with (n = 15) and without (n = 15) AV block using propensity score‐matching. A WCL≥400ms was associated with a 4‐fold higher incidence of AV block (4.79% vs. 1.25%).ConclusionIncreased pre‐procedural WCL was associated with a high risk for AV block after catheter ablation treatment for AVNRT. These findings suggest that this readily available EPS‐derived parameter may be a novel marker of risk for severe complications in these patients.
High anatomical accuracy of a novel high‐resolution wide‐band dielectric imaging system in cryoballoon‐based ablation
Authors: Rottner Laura , Nodorp Milena , Jessica Weimann , Schleberger Ruben , Sinning Christoph , Lemoine Marc , Dinshaw Leon , Münkler Paula , Lin Tina , Meyer Christian , Reissmann Bruno , Metzner Andreas , Rillig Andreas 
Published: July 2021

AbstractPurposeRecently, a novel cardiac imaging system based on a wide‐band dielectric technology (KODEX‐EPD) was introduced to guide catheter ablation. The aim of the study was to evaluate this 3D wide‐band dielectric imaging system (WDIS) during cryoballoon (CB)‐based atrial fibrillation (AF) ablation focusing on accuracy of pulmonary vein (PV)‐anatomy.MethodsIn consecutive patients with symptomatic AF, CB‐based ablation was performed in conjunction with the 3D WDIS. Selective PV‐angiographies were performed, and 3D anatomy of the left atrium (LA) and PVs using the 3D WDIS was created. The ostial diameters of the ipsilateral right‐sided and left‐sided PVs and ostial diameters of the right‐/left‐sided upper/lower PVs demonstrated by selective angiographies were analyzed and compared to 3D WDIS‐based PV visualization.ResultsIn 65 patients (42/65 (65%) male, age 65 ± 9 years, 29/65 (45%) paroxysmal AF) a total of 260 PVs were identified. Median ostial PV‐diameters for the ipsilateral left‐ and right‐sided PVs were 38 [34; 43] and 37 [34; 40.3] mm when assessed fluoroscopically and 40 [35.7; 43] and 39 [35.0; 43] mm as demonstrated by 3D WDIS. There was no statistically significant difference between both methods regarding PV‐diameter measurements. KODEX‐EPD overestimated fluoroscopy measurements by 1.08 mm (95% limits of agreement of ‐1.93 mm and 4.1 mm).ConclusionThe novel wide‐band dielectric 3D‐imaging system is feasible to create high‐resolution images of cardiac structures during CB ablation procedures and accurately visualizes PV‐anatomy.
Exploring the diversity of promoter and 5′UTR sequences in ancestral, historic and modern wheat
Authors: Hammond‐Kosack Michael C.U. , King Robert , Kanyuka Kostya , Hammond‐Kosack Kim E. 
Published: July 2021

AbstractA data set of promoter and 5′UTR sequences of homoeo‐alleles of 459 wheat genes that contribute to agriculturally important traits in 95 ancestral and commercial wheat cultivars is presented here. The high‐stringency myBaits technology used made individual capture of homoeo‐allele promoters possible, which is reported here for the first time. Promoters of most genes are remarkably conserved across the 83 hexaploid cultivars used with <7 haplotypes per promoter and 21% being identical to the reference Chinese Spring. InDels and many high‐confidence SNPs are located within predicted plant transcription factor binding sites, potentially changing gene expression. Most haplotypes found in the Watkins landraces and a few haplotypes found in Triticum monococcum, germplasms hitherto not thought to have been used in modern wheat breeding, are already found in many commercial hexaploid wheats. The full data set which is useful for genomic and gene function studies and wheat breeding is available at https://rrescloud.rothamsted.ac.uk/index.php/s/DMCFDu5iAGTl50u/authenticate.
Development of the Polish‐Language Relationship Disillusionment Scale and its validation
Authors: Niehuis Sylvia , Adamczyk Katarzyna , Trepanowski Radosław , Celejewska Agata , Ganclerz Monika , Frydrysiak Aleksandra , Reifman Alan , Willis‐Grossmann Emma 
Published: July 2021

AbstractIn Poland, individuals enter marriages and relationships increasingly to derive personal fulfillment, creating high expectations that may elevate individuals' risk for romantic disillusionment (the perception that the relationship has not lived up to expectations and has deteriorated). Given these dynamics in Poland, a Polish‐language measure of disillusionment would open up new research opportunities. Three studies, therefore, examined psychometric properties of a new Polish‐Language Relationship Disillusionment Scale (RDS‐PL), which was translated and back‐translated with an established English‐language measure. Study 1 (pilot study) administered the English‐ and Polish‐language RDS versions 2 months apart to 19 bilingual Polish university students, yielding cross‐language test–retest reliability of 0.61. In Study 2, 214 individuals completed the RDS‐PL and other Polish‐language measures. Confirmatory factor analyses supported a one‐factor RDS‐PL structure, and significant correlations of the RDS‐PL with relationship satisfaction, forgiveness, and resentment provided convergent validity. Study 3 (N = 280) supported the RDS‐PL's convergent validity with English and Polish‐language relationship measures. Studies 2 and 3 also partially supported its discriminant validity.
The Recent Politicization of Immigration in Poland in Light of Preexisting State Practices: Continuity or Change in the Understanding of Citizenship and Nationhood?
Authors: Skowronska Kaja 
Published: July 2021

By drawing a division between citizens and noncitizens, migration policies speak to implicit definitions of what makes a good citizen. Commonly accepted definitions of citizenship are produced not only through explicit political discourse but also through the everyday actions of state agencies. The selection of the “worthy” migrants that those street‐level bureaucracies operate is deeply rooted in interpretations of the nation's boundaries. In this article, I examine this everyday production of citizenship and nationhood by focusing on the case of Polish migration policies approached through the lens of an ethnographic study of street‐level bureaucracies. While the rapid politicization of immigration in this country since 2015 resembles a major shift, an inquiry into the practices of policy implementation invites interrogations concerning continuities between past and present state practices, as well as concerning the definitions of citizens and of “worthy” immigrants that were and are prevalent within Polish institutions.Related Articles in this Special IssueBarrault‐Stella, Lorenzo, and Thomas Douniès. 2021. “Introduction to the Special Issue: Citizenship as a Tool of Government in Europe.” Politics & Policy 49 (4).Haapajärvi, Linda. 2021. “On the Importance of Playing House: Belonging Work and the Making of Relational Citizens in Finnish Immigrant Integration Policies.” Politics & Policy 49 (4).Mioni, Michele. 2021. “The ‘Good Citizen’ as ‘Respectable Worker:’ State, Unemployment, and Social Policy in the United Kingdom and Italy, 1930 to 1950.” Politics & Policy 49 (4).
Communication about physical activity to reduce vascular erectile dysfunction – A qualitative interview study among men in cardiac rehabilitation
Authors: Gerbild Helle , Areskoug Josefsson Kristina , Marie Larsen Camilla , Schantz Laursen Birgitte 
Published: July 2021

AbstractBackgroundPhysical activity, a core intervention in cardiac rehabilitation, can reduce vascular erectile dysfunction (ED). ED is a common sensitive problem for men with cardiac diseases, decreasing their quality of life. Cardiac health professionals rarely provide information about ED or its relation to physical activity. Developing health professionals’ communicative component of the complex intervention ‘Physical Activity to reduce Vascular Erectile Dysfunction’ (PAVED) is important. Understanding the receiver needs is essential in designing a complex intervention.AimTo elucidate men's perspectives on cardiac health professionals’ communication about PAVED.Ethical issuesAn Institutional Data Protection Agency approved the study.MethodsAn interpretive data‐driven thematic analysis was applied to individual, qualitative semi‐structured interviews with 20 Danish men attending cardiac rehabilitation.ResultsThe men wanted health professionals’ communicating about ED, as it was perceived as a major problem diminishing masculinity and tabooed by health professionals. Men wanted help for self‐help, which may be possible with the aid of competent health professionals’ communication about how to prevent, reduce and cope with ED ‐ including information about PAVED. The men wanted health professionals’ communication about ED in various contexts: general information in groups, sexual counselling for individuals and couples and written material.Study limitationsRecruitment was done from a Danish municipality's cardiac rehabilitation, and the transferability of the results may be limited to similar contexts.ConclusionErectile dysfunction was experienced as a major biopsychosocial problem for the men and their partners. The men had a need for health professionals’ communication about sexuality, ED and information about PAVED as well as about prevention, reduction and management of ED. The men had a need for professional communication about sexual health.