bluuebunny/crossref_metadata_2025_split huggingface dataset:
Primary Catheter-Directed Thrombolysis For Porto-Mesenteric Venous Thrombosis (PMVT) In Non-Cirrhotic Patients.
Authors: Chiang Chia-Ling , Liang Huei-Lung , Li Ming-Feng 
Published: November 2021

Abstract
Purpose: To report our technique, treatment strategy and clinical outcomes for porto-mesenteric venous thrombosis (PMVT) in non-cirrhotic patients.Methods: 16 non-cirrhotic PMVT patients (mean age: 48.6 years) with imminent intestinal ischemia were enrolled from 2004-2020. 8 patients presented thrombus extension into the peripheral mesenteric vein, close to the venous arcade. Transhepatic catheter-directed thrombolysis (CDT) was performed by urokinase infusion (60,000-30,000 IU/hour concomitant with heparin 300-400 IU/hour), catheter aspiration and/or balloon dilation/stent placement. Additional intra-arterial mesenteric infusion of urokinase (30,000 IU/hour) was given in patients with the peripheral mesenteric venules involved. Transjugular intrahepatic porto-systemic shunt (TIPS) was created in patients with poor recanalization of the intrahepatic portal flow (PV). Results: The transhepatic route was adopted in all patients, with adjunct indirect mesenteric arterial thrombolytic infusion in 8 patients. A total of up to 20.4 million IU urokinase was infused for 1-30 days’ treatment duration. TIPS was created in 3 patients with recanalization failure of the intrahepatic PV. Technical success was achieved in 100% of patients with complete recanalization of 80% and partial recanalization of 20%. No major procedure-related complications were encountered. The 30-day mortality rate was 6.7%. The overall 1- and 2-year primary and secondary patency were both 74.0% and 84.6% respectively. Conclusions: CDT can be performed as a primary salvage treatment once the diagnosis is made. CDT via the transhepatic route with tailored thrombolytic regimen is safe and effective for both acute and chronic PMVT . TIPS creation can be preserved in non-cirrhotic PMVT patients if intrahepatic PV recanalization fails.
Mad7: An IP Friendly CRISPR Enzyme
Authors: Rojek Johan , Basavaraju Yogesh , Nallapareddy Saranya , Baumgartner Roland Franz , Schoffelen Sanne , Pedersen Lasse Ebdrup 
Published: November 2021

Abstract
Mad7 is a CRISPR enzyme, similar to Cas9 and Cas12a, which is of great interest to industry and academia due to its permissive licensing agreement, which states that everyone is free to use it but not distribute it commercially. This paper demonstrates that Mad7 genome editing works in CHO cells and provides the CHO engineering community with the computational tools needed to generate Mad7 compatible gRNAs against the CHO genome.
Preparation and Comparative Study of Aerogels Based on Cellulose Nanocrystals and Nanofibers from Eucalyptus Pulp
Authors: Zhu Wenkai , Zhang Yang , Wang Xiaoyu , Wu Yan , Han Minsu , You Jungmok , Jia Chong , Kim Jeonghun 
Published: November 2021

Abstract
Nanocellulose-based materials have attracted significant attention because of their attractive advantages. Particularly, aerogel, a porous nanocellulose material, have been used in diverse applications owing to their unique properties. In this study, short rod-like cellulose nanocrystals (CNCs) and long filament-like cellulose nanofibers (CNFs) were isolated from a eucalyptus pulp source using acidolysis and oxidation/mechanical methods, respectively. Subsequently, two different aerogels were prepared from the CNCs and CNFs using the sol-gel method and their properties were compared. The morphology, chemical structure, chemical composition, shrinkage rate, internal structure, thermal degradation, biophysical properties, and mechanical properties of the as-prepared aerogels were compared. Furthermore, the shrinkage of the CNC and CNF aerogels was effectively controlled using a supercritical CO2 drying process. Additionally, three decomposition regions were observed in the thermogravimetric analysis curves of the aerogels; however, the CNF aerogels exhibited enhanced thermal stability than the CNC aerogels. Further, the CNC and CNF aerogels exhibited a mesoporous structure, and the compressive strength of the CNC and CNF aerogels under 85% strain was 269.5 and 299.5 KPa, respectively. This study provides fundamental knowledge on the fabrication of CNCs, CNFs, and corresponding aerogels from lignocellulosic biomass, and their characteristics.
Association of microRNA-652 Expression with Radiation Response of Colorectal Cancer: A Study from Rectal Cancer Patients in a Swedish Trial of Preoperative Radiotherapy to Public Data Analysis and in Vitro Investigation
Authors: Pathak Surajit , Meng Wen-Jian , Sriram Sushmitha , Jangamreddy Jaganmohan Reddy , Banerjee Antara , Jothimani Ganesan , Adell Gunnar , Zhang Xueli , Zhang Alexander Sun , Zhang Hong , Sun Xiao-Feng 
Published: November 2021

Abstract
Purpose: Radiotherapy (RT) is a standard adjuvant therapy in progressive rectal cancer patients, but many patients are resistant to RT, leading to poor prognosis. Our study identified microRNA-652 (miR-652) value on RT response and outcome in rectal cancer patients.Methods: miR-652 expression was determined by RT-PCR in primary rectal cancer from 48 patients with and 53 patients without RT. The relationship of miR-652 with biological factors and prognosis were examined. The biological function of miR-652 was identified through TCGA and GEPIA database search. Two human colon cancer cell lines (HCT116 p53+/+ and p53-/-) were used for in vitro study. Results: miR-652 expression was augmented significantly in cancer than normal mucosa in non-RT patients (P=0.044). High miR-652 expression in non-RT patients was related to more apoptosis (P=0.036), ATM (P=0.010) and DNp73 expression (P=0.009). High miR-652 expression was related to worse disease-free survival of non-RT patients, independent of gender, age, tumor stage and differentiation (P=0.028; HR=7.398, 95% CI 0.217-3.786). The biological functional analysis further identified the prognostic value and potential relationship of miR-652 with the apoptosis in rectal cancer. In RT patients, miR-652 expression was notably decreased in cancers when compared to non-RT cases (P=0.047), and miR-652 expression in cancers was negatively related to WRAP53 expression (P=0.022). After miR-652 inhibition, the estimation of reactive oxygen species, caspase activity and apoptosis in HCT116 p53+/+ cells were significantly increased compared with HCT116 p53-/- cells after radiation.Conclusions Our findings suggest the potential value of miR-652 expression as a marker for the prediction of radiation response and clinical outcome in rectal cancer patients.
Multi-Objective Casting Production Scheduling Problem by a Neighborhood Structure Enhanced Discrete NSGA-II: An Application from Real-World Workshop
Authors: Tan Weihua , Yuan Xiaofang , Yang Yuhui , Wu Lianghong 
Published: November 2021

Abstract
Casting production scheduling problem (CPSP) has attracted increasing research attention in recent years to facilitate the profits, efficiency, and environment issues of casting industry. Casting is often characterized by the properties of intensive energy consumption and complex process routes, which motivate the in-depth investigation on construction of practical multi-objective scheduling models and development of effective algorithms. In this paper, for the first time, the multi-objective casting production scheduling problem (MOCPSP) is constructed to simultaneously minimize objectives of defective rate, makespan, and total energy consumption. Moreover, a neighborhood structure enhanced discrete NSGA-II (N-NSGA-II) is designed to better cope with the proposed MOCPSP. In the N-NSGA-II, the advantage of selection mechanism of NSGA-II is fully utilized for selecting non-dominate solution, three neighborhood structures are elaborately designed to strengthen the ability of the local search, and a novel solution generating approach is proposed to increase the diversity of solutions for global search. Finally, a real-world case is illustrated to evaluate the performance of the N-NSGA-II. Computational results show that the proposed N-NSGA-II obtains a wider range of non-dominated solutions with better quality compared to other well-known multi-objective algorithms.
Infantil Hemangioma and Optimum Dose of Propranolol Treatment: A Retrospective Tertiary-Center Study
Authors: Cakmak Hatice Mine , Kartal Omer 
Published: November 2021

Abstract
Background/Objectives: Propranolol is the mainstay treatment of infantile hemangioma, and the optimal dose is unclear. Few studies are comparing the efficacy of propranolol dose of 2 vs.3 mg/kg/day. We compared the efficacy between these two doses and propranolol groups with no treatment group. Methods: One hundred eight patients with infantile hemangioma (15 days-27 months of age) were examined. The patients with high-risk features and/or a score of >6 points are given propranolol with a final dose of 2 or 3 mg/kg/day according to tolerance for 6-12 months. The resolutions rates for propranolol vs. placebo and propranolol 2 mg/kg/day vs. 3 mg/kg/day are compared. Results: The demographic and clinical features of the groups ( the non-treatment, propranolol 2 mg/kg/day group, propranolol 3 mg/kg/day group) are similar. Propranolol is significantly efficent in infantil hemangioma treatment (p<0.001). The resolution rates are not statistically different between 2 mg/kg/day propranolol group vs 3 mg/kg/day propranolol group at the sixth (68,59 ± 28,95 vs 73,44 ± 32,54)(p=0,673) and twelfth month (p=0,673) (89,08 ± 46,58 vs 91,13 ± 37,46 respectively )of follow up. A milld (n=3)(4%) adverse event was reported with no need for cessation.Conclusions: Propranolol is a safe drug for treating infantile hemangioma with an ideal dose of 2 mg/kg/day rather than 3 mg/kg/day.
Analysis of Automatically Extracted Hallux Valgus Angles Using Markerless 3D Foot Scans From North America, Europe and Asia
Authors: Jiao Yang , Džeroski Sašo , Jurca Ales 
Published: November 2021

Abstract
Background The forefoot is the foot most affected part by ill-fitting shoes. Footwear fitting standards have the measurements of length, width and arch length. Toe shape has not yet been used in footwear measurement. This study aims at investigating the variation in toe shape, as measured by the hallux valgus angle. Methods An automatic and reproducible hallux valgus angle measuring method using 3D foot scans with no palpation markers is proposed and applied to about half a million samples collected across North America, Europe and Asia. The measuring method is robust, it can detect the medial contour along the proximal phalanx even in extreme cases. Results The hallux valgus angle has a normal distribution with long tails on both sides in the general population. In the three regions (North America, Europe, Asia), the mean and standard deviation of this angle are 9° ± 6°, 8 ° ± 6°, 12° ± 6° for males and 11 ° ± 7°, 12 ° ± 7°, 16° ± 7° for females. Conclusions The hallux valgus angle has a broad distribution in the general population. Females have larger hallux valgus angle than males, and people from Asia have larger hallux valgus angle than people from North America and Europe.
Classify Elderly Pain Severity from Open-Source Automatically Video Clip Facial Action Units Analysis. A Study from the Integrated Pain Artificial Intelligence Network (I-PAIN) Data Repository.
Authors: Gomutbutra Patama , Kittisares Adisak , Sanguansri Atigorn , Choosri Noppon , Sawaddiruk Passakorn , Fakfum Puriwat , Lerttrakarnnon Peerasak , Saralamba Sompob 
Published: November 2021

Abstract
Background: It is increasingly interesting to monitor pain severity in elderly individuals by applying machine learning models. In previous studies, OpenFace© - a well-known automated facial analysis algorithm, was used to detect facial action units (FAUs) that initially need long hours of human coding. However, OpenFace© developed from the dataset that dominant young Caucasians who were illicit pain in the lab. Therefore, this study aims to evaluate the accuracy and feasibility of the model using data from OpenFace© to classify pain severity in elderly Asian patients in clinical settings.Methods: Data from 255 Thai individuals with chronic pain were collected at Chiang Mai Medical School Hospital. The phone camera recorded faces for 10 seconds at a 1-meter distance briefly after the patients provided self-rating pain severity. For those unable to self-rate, the video was recorded just after the move, which illicit pain. The trained assistant rated each video clip for the Pain Assessment in Advanced Dementia (PAINAD). The classification of pain severity was mild, moderate, or severe. OpenFace© process video clip into 18 FAUs. Five classification models were used, including logistic regression, multilayer perception, naïve Bayes, decision tree, k-nearest neighbors (KNN), and support vector machine (SVM). Results: Among the models that included only FAU described in the literature (FAUs 4, 6, 7, 9, 10, 25, 26, 27 and 45), multilayer perception yielded the highest accuracy of 50%. Among the machine learning selection features, the SVM model for FAU 1, 2, 4, 7, 9, 10, 12, 20, 25, 45, and gender yielded the best accuracy of 58%. Conclusion: Our open-source automatic video clip facial action unit analysis experiment was not robust for classifying elderly pain. Retraining facial action unit detection algorithms, enhancing frame selection strategies, and adding pain-related functions may improve the accuracy and feasibility of the model.
Evaluation the Reactivity of a Peptide-Based Monoclonal Antibody with Drug Resistant Pulsotypes of Acinetobacter Baumannii as Potential Therapeutic Approach
Authors: Yeganeh Omid , Shabani Mahdi , Pakzad Parviz , Mosaffa Nariman , Hashemi Ali 
Published: November 2021

Abstract
Background:

Acinetobacter baumannii is an opportunistic and antibiotic-resistant pathogen that predominantly causes nosocomial infections. There is urgent need for development nonantibiotic-based treatment strategies. We developed novel monoclonal antibody (mAb) against a peptide of conserved outer membrane protein A (OmpA) and evaluated its reactivity with different pulsotypes of A.baumannii.
Materials and Methods:

Peptide derived from A.baumannii OmpA was conjugated to keyhole limpet hemocyanin and injected into Balb/c mice. Splenocytes of immunized mice were fused with SP2/0 myeloma cells followed by selection of antibody-producing hybridoma cells. After screening of different hybridoma colonies by ELISA, one monoclone was selected as 3F10-C9 and the antibody was tested for reaction with five different Acinetobacter pulsotypes that were resistant to carbapenem antibiotics. The affinity constant was measured by ELISA. The ELISA, Western blotting, indirect immunofluorescence (IFA), and in vitro Opsonophagocytosis assays were used to evaluate the reactivity of generated mAb.
Results:

The anti-OmpA antibody reacted with the immunizing peptide and had a high affinity (around 1.94 × 10 − 9 M) for its antigen in the ELISA. Specific binding of mAb to OmpA was confirmed in Western blot. IFA assays revealed that mAb recognized specific OmpA on the pulsotypes. Opsonophagocytosis assays showed that the mAb increased bactericidal activity of macrophage cells. The antibody function was higher in the presence of serum complement.
Conclusion:

The peptide-based mAb demonstrated optimal performance in laboratory experiments which may be appropriate in investigation on OmpA in Acinetobacter pathogenesis and development of passive immunization as novel therapeutic approach.
The Effects of the Probiotic Cocktail On Modulation of the NF-kB and JAK/STAT Signaling Pathways Involving in Inflammatory Response in Bowel Disease Model
Authors: Aghamohammad Shadi , Sepehr Amin , Miri Seyedeh Tina , Najafi Saeideh , Rohani Mahdi , Pourshafie Mohammad Reza 
Published: November 2021

Abstract
Background: Probiotics are beneficial microorganisms which have beneficial effects on inflammatory responses, in part, through Janus kinase/signal transduction and activator of transcription (JAK/STAT) and inflammatory signaling pathways. Materials: Quantitative real-time polymerase chain reaction (qPCR) was used to analyze the expression of JAK/STAT and inflammatory genes (TIRAP, IRAK4, NEMO, and RIP) following treatment of HT-29 cell line with sonicated pathogen, Lactobacillus spp., Bifidobacterium spp. and a mixture cocktail. Cytokine assay also used to evaluate the production of IL-6 and IL-1B following the probiotic treatment. Results: Compared to sonicate pathogen treatment cells, our probiotics cocktail was down-regulated JAK genes and TIRAP, IRAK4, NEMO, and RIP genes in NF-kB pathway. The expression of STAT genes were variable following the probiotic treatment. The production of IL-6 and IL-1B were decreased after probiotic treatment. Conclusions: Our probiotics cocktail was shown to have anti-inflammatory effects on HT-29 cells by modulating JAK/STAT and NF-kB pathways; therefore, the use of Lactobacillus spp. and Bifidobacterium spp. probiotics as nutritional supplements may reduce inflammation-associated diseases such as inflammatory bowel disease.
Expression of the Yes-Associated Protein, β-Catenin, and Smoothened Protein and Their Clinical Significance in Invasive Breast Cancer
Authors: Liu Pengju , Zeng Jianfeng , Yang Gaohua 
Published: November 2021

Abstract
Background: The mechanism of the Yes-associated protein in the occurrence and development of breast cancer is still unclear. Here, we aimed to explore the relationship among the Yes-associated protein, β-catenin, and Smoothened signaling pathways to provide a theoretical basis for the clinical diagnosis and Treatment program of invasive breast cancer. Methods: Immunohistochemistry was used to detect the expression of Yes-associated protein, Smoothened protein, and β-catenin in breast cancer, adjacent, and normal tissues. The relationship between the expression of these three proteins and clinicopathological features of breast cancer patients was analyzed, and western blotting was used to detect differences in Yes-associated protein expression in MCF-7 and MDA-MB-231 cell lines. Results: The expression level of Yes-associated protein in breast cancer tissues was prominently Lower than that in normal breast tissues; the expression level of β-catenin in breast cancer tissues was prominently higher than that in normal tissues; and the expression level of Smoothened protein in cancer tissue was Prominently higher than that in normal breast tissue. The expression of Yes-associated protein in breast cancer tissues was correlated with the expression of the human epidermal growth factor receptor 2, progesterone receptor, and estrogen receptor; the expression of β-catenin and Smoothened protein in breast cancer tissues were all correlated with the human epidermal growth factor receptor expression. The expression level of Yes-associated protein in the MCF-7 cells was significantly higher than that in the MDA-MB-231 cells. There was a negative correlation between the Yes-associated protein and Smoothened protein expression in the breast cancer tissues.Conclusions: The Yes-associated protein, β-catenin, and Smoothened protein can be used as new targets in the treatment of invasive breast cancer or as reference indicators for prognostic judgment. The differences in expression of the three proteins in cancer adjacent, cancer, and normal tissues suggest that they may be of great significance in the process of breast-conserving surgery.
SLR Estimation Using CEEMDAN Near the Korean Peninsula
Authors: Kim Young Jin , Kwon Okyu , Song Hark-Soo , Kim Jongho , Kang Hyuk 
Published: November 2021

Abstract
The rise of sea levels due to global warming is a problem of concern at an international scope and the causes are already known relatively clearly. Every year, the Intergovernmental Panel on Climate Change (IPCC) creates a scenario for greenhouse gas emissions and predicts the global average sea-level rise rate accordingly. It is necessary to estimate the rate of sea-level rise to date in creating such a scenario. In particular, since the height of the sea level changes (SLC) continuously, the errors of SLC may occur due to various causes with a fragmental analysis. To estimate the sea-level rise accurately, we applied Complete Ensemble Empirical Mode Decomposition with Adaptive Noise (CEEMDAN) is based on the Empirical Mode Decomposition (EMD) to decompose the tidal level. Through this, we discover that the differences in the local sea-level rise rate occurred even within a small area. To understand each component of tide level decomposed through CEEMDAN, we confirm the component-wise/regional correlation between tidal stations. In addition, we looked at how local sea-level rise correlated with the global meteorological phenomenon, El Niño-Southern Oscillation (ENSO) which is one of the most influential recurring climate patterns Socioeconomically.
Hand-Held Dynamometry to Safely Measure Muscle Strength in Patients In Intensive Care
Authors: Júnior Balbino Rivail Ventura Nepomuceno , Santos Kamilla Ribeiro Bastos dos , Júnior Jailson de Souza Santos , de Almeida Deisimar Fernandes , de Barros Roberto Moreno , Neto Mansueto Gomes 
Published: November 2021

Abstract
Background: Intensive care unit-acquired weakness (ICU-AW) has been increasingly studied and associated with prognostic factors of negative outcomes during the hospitalization of critically ill patients. Therefore, it is essential to evaluate the muscle strength of patients in the intensive care unit (ICU) with accurate quantitative tools, such as the hand-held dynamometer (HHD), which directly measures the strength of large muscle groups, providing earlier and more accurate decision making. The objective of the present study was to evaluate the safety of using HHD to measure the strength of large muscle groups in ICU patients, in addition to determining the prevalence of muscle imbalance in these patients, and correlating HHD measures with the Medical Research Council (MRC) scale. Methods: A cross-sectional study was conducted, inferring the impact of strength measurement of the main muscle groups using HHD on vital signs, dyspnea, and pain. The occurrence of adverse events during the evaluation was also observed. Safety was assessed using the paired t-test, calculating the prevalence of muscle imbalance in the sample and Pearson’s correlation between the strength measurement instruments. Results: The sample consisted of 46 volunteers, and no clinically significant variability was observed for pre- and post-safety variables. A high prevalence of muscle imbalance was found in the sample, and there was a strong correlation between HHD and MRC. Discussion: Strength measurement using HHD in the ICU is safe and well-tolerated, providing information on the individual condition of large muscle groups and improving the ability to diagnose muscle imbalance in the ICU patients.