bluuebunny/crossref_metadata_2025_split huggingface dataset:
Colon Available Bioactive Compounds Exhibits Anticancer Effect onIn-VitroModel of Colorectal Cancer
Authors: Patil Poounima , Killedar Suresh 
Published: May 2021

The current work was addressed to characterize gallic acid from amla fruit and quercetin from peels of pomegranate fruit and formulated into Chitosan (CS) nanoparticles and to evaluate their cytotoxicity towards human colorectal cancer (HCT 116) cell lines. Identification of the biomolecules was performed by chromatographic and spectroscopic techniques and characterization of gallic acid and quercetin loaded chitosan nanoparticles carried out by using FT-IR, X- ray diffraction, entrapment efficiency and loading content confirmed successful encapsulation of biomolecules into nanoparticles. In vitro drug release studies done by using simulated fluids at various pH (1.2, 4.5, 7.5, and 7.0) to mimic the GIT tract and achieved drug releases 77.56% for gallic acid 79.06% for quercetin at 24 hr. in a sustained manner. The human HCT116 cell line by MTT assay results inferred that the synthesized CS nanoparticles demonstrated shows more effective antiproliferative potential with IC50 value of 36.17 ug/ml than polyherbal extract 60.32ug/ml.
The Conservation of European Goldfinch in North Algeria
Authors: Mouslim Bara 
Published: May 2021

This chapter presents the conservation status and threat of the European goldfinch Carduelis carduelis in Algeria. Many selective pressures run into this passerine, mainly human pressure due to keeping and hatching the birds in captivity. Illegal trading is actively carried out between countries of North Africa (mainly the pathway Morocco – Algeria – Tunisia). This situation is clearly expounding a threatened status of this songbird. A scientific survey was done in north center of Algeria to assess the captivity rate of goldfinch. Systematic sampling was done in several houses of Bouira district. Goldfinch breeder’s age varies between 20 and 40 years. No income and no fixed job are the main reasons for these breeders. Results exposed serious threat of this species in center of Algeria. Management plan and conservation directions have been proposed for this species.
Modelado de distribución de especies en los bosques de los andes meridionales
Authors: Alberdi Nieves Virginia 
Published: May 2021

La elevada biodiversidad junto con una gran variedad de ecosistemas convierten a la Cordillera de los Andes es una de las regiones de mayor diversidad ambiental del mundo, donde se encuentran los rangos más extremos de tipos de paisajes, clima y formaciones forestales de la Tierra, un área adecuada para estudiar los posibles efectos del cambio climático sobre la distribución espacial de las formaciones forestales.
Para ello resulta imprescindible entender los efectos de cambio climático en la zona, dónde las observaciones climáticas indican diferentes escenarios climáticos en el futuro, para un periodo de tiempo actual y para el periodo 2040-2069, con variaciones de las temperaturas y precipitaciones. Se analiza la distribución de los bosques a través de modelado predictivo con el método de máxima entropía de MaxEnt. Los resultados señalan que la mayoría de las formaciones forestales de los bosques andinos analizados tendrán previsiblemente problemas importantes en un futuro próximo, consecuencia de la pérdida de idoneidad climática en el área actual de distribución y del cambio geográfico de las áreas potencialmente adecuadas en el futuro como reflejan los resultados.
Palabras clave: biodiversidad, modelos de distribución de especies, cambio climático, bosques, máxima entropía.
The high biodiversity along with a great variety of ecosystems turn Andes Mountain one of the regions of greater environmental diversity of the world, where are the most extreme ranges of types of landscapes, climate and forest formations of the Earth, an area suitable for studying the possible effects of climate change on the spatial distribution of forest formations.
For this it is essential to understand the effects of climate change in the area, where climate observations indicate different climate scenarios in the future, for a current time period and for the period 2040-2069, with variations in temperatures and precipitation. The distribution of forests through predictive modelling is analyzed using MaxEnt’s maximum entropy method. The results indicate that most of the forest formations in the Andean forests analysed are expected to face significant problems in the near future, as a result of the loss of climate suitability in the current area of distribution and the geographic change of potentially suitable areas in the future as reflected in the results.
Keywords: biodiversity, species distribution models, climate change, forests, maximum entropy
Cuenca hidrográfica agropecuaria y evolución de los servicios ecosistémicos en la región sur pampeana Argentina
Authors: Vazquez Patricia , Somoza Ailin 
Published: May 2021

Los modelos productivos actuales incluyen doble cultivo anual, aumento de agroquímicos, fragmentación de la biodiversidad y alteración de ecosistemas. El objetivo del presente trabajo es analizar de forma temporal las transformaciones de los servicios ecosistémicos (SE) de las unidades agroecológicas (UAE) de la Cuenca del río Quequén Grande (CrQG). La metodología se basa en la estimación de indicadores de sustentabilidad: conservación de la calidad de suelos y agua (CCSA), conservación de la biodiversidad (CB) y conservación del ecosistema (CE). La metodología utilizada fue el cálculo de las ecuaciones de los indicadores por UAE (a partir de datos de trabajos antecedentes y observación actual directa a campo), y se calcularon coeficientes de correlación lineal (r) entre los indicadores. Los resultados muestran que el proceso de agriculturización afecta sobre todo a los servicios de regulación de la unidad de Sierras y Serranías, y que en todas las UAE los ecosistemas fueron adaptados en su mayor parte a la provisión de servicios de abastecimiento de importancia comercial. Los gráficos de dispersión permitieron verificar correspondencia en los valores. Se concluye que los SE se ven afectados de diferente manera al interior de la CrGQ siendo fundamental una gestión que permita un futuro ordenamiento territorial.
The Pampean Region has strong comparative advantages for agricultural production. Current production models include annual double cultivation with increased use of agrochemicals and strong alteration of ecosystems with biodiversity fragmentation. The aim of this work is to temporarily analyze the transformations of the ecosystem services (SE) of the agroecological units (UAE) of the Quequén Grande River Basin (CrQG). The methodology is based on the estimation of sustainability indicators: soil and water quality conservation (CCSA), biodiversity conservation (CB) and ecosystem conservation (CE).
For these, surface data obtained from classified images on the advancement of agricultural development by UAE of the Basin and field data obtained in interviews and background studies are necessary. The results show that the agricultural instensification process was manifested with different intensity in the different UAE of the Basin, the Sierras and Serranías unit was the most affected in both periods, with high values of environmental risk. The conclusions are that SE are affected differently in the CrQG and therefore it is intended to contribute to the territorial planning of SE in the basin.
Molecular evidence for cross boundary spread ofSalmonellaspp. in meat sold at retail markets in the middle Mekong basin area
Authors: Meunsene Dethaloun , Eiamsam-ang Thanaporn , Patchanee Prapas , Pascoe Ben , Tadee Phacharaporn , Tadee Pakpoom 
Published: May 2021

BackgroundThe surrounding areas of the middle Mekong basin, particularly along the border between Thailand and Lao People’s Democratic Republic (Lao PDR), are high-risk areas for many livestock-associated foodborne illnesses, especially salmonellosis. This study aimed to determine the prevalence and characteristics ofSalmonellaspp. contamination in pork, beef and chicken meats sold at retail markets in the Thailand-Laos border area surrounding the Thai-Lao Friendship Bridge I from January to May 2019. We focused on the prevalent serotypes, antimicrobial susceptibility profiles and the multilocus sequence type (MLST) genotypes of the collectedSalmonellastrains.ResultsFrom a total of 370 meat samples collected, 63% were positive forSalmonella, with the prevalence of 73%, 60% and 56% from pork, beef and chicken meat samples, respectively. Of all the positive samples, 53 serotypes were identified. Of these,Salmonella entericaserovar London accounted for the majority (27%), followed by serovars Corvallis (14%), and Rissen (6%). Resistance against tetracycline was found at the highest frequency (50%), followed by ampicillin (35%) and sulfamethoxazole-trimethoprim (28%). MLST revealed no evidence of shared genetic relatedness ofSalmonellaat retail sites among Thailand-Laos border zone. However, a diverse range ofSalmonellagenotypes were spread over the area. Besides, the persistence of the residential pathogen and sharing of the supply route within-country can be inferred.ConclusionsGiven the high levels of contamination of retail meats, regular disinfecting of all working areas and quality control checking at pre-retail stage must be applied to reduce the transmission ofSalmonellaand other foodborne pathogens to consumers. The findings of this study will make a significant contribution to the current understanding ofSalmonellaepidemiology to enhance food security in the region.
Centromere protein N may be a novel malignant prognostic biomarker for hepatocellular carcinoma
Authors: Wang Qingqing , Yu Xiaoyan , Zheng Zhewen , Chen Fengxia , Yang Ningning , Zhou Yunfeng 
Published: May 2021

Background
Hepatocellular carcinoma (HCC) is one of the deadliest tumors. The majority of HCC is detected in the late stage, and the clinical results for HCC patients are poor. There is an urgent need to discover early diagnostic biomarkers and potential therapeutic targets for HCC.

Methods
The GSE87630 and GSE112790 datasets from the Gene Expression Omnibus (GEO) database were downloaded to analyze the differentially expressed genes (DEGs) between HCC and normal tissues. R packages were used for Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO) enrichment analyses of the DEGs. A Search Tool for Retrieval of Interacting Genes (STRING) database was used to develop a protein-protein interaction (PPI) network, and also cytoHubba, Molecular Complex Detection (MCODE), EMBL-EBI, CCLE, Gene Expression Profiling Interactive Analysis (GEPIA), and Oncomine analyses were performed to identify hub genes. Gene expression was verified with a third GEO dataset, GSE25097. The Cancer Genome Atlas (TCGA) database was used to explore the correlations between the hub genes and clinical indexes of HCC patients. The functions of the hub genes were enriched by gene set enrichment analysis (GSEA), and the biological significance of the hub genes was explored by real-time polymerase chain reaction (qRT-PCR), western blot, immunofluorescence, CCK-8, colony formation, Transwell and flow cytometry assays with loss-of-function experiments in vitro.

Results
Centromere protein N (CENPN) was screened as a hub gene affecting HCC tumorigenesis. Evaluation by Cox regression showed that a high level of CENPN expression was an independent danger variable for poor prognosis of HCC. GSEA showed that high CENPN expression was linked to the following pathways: liver cancer subclass proliferation, cell cycle, p53 signaling pathway, Rb1 pathway, positive regulation of cell cycle G1/S phase transition, and DNA damage response signal transduction by p53 class moderators. Further cell experiments showed that knocking down CENPN expression decreased the proliferation and colony-forming abilities of HepG2 and Huh7 cells as well as Ki67 expression in these cell lines. The cell cycle was arrested in G1 phase, which is consistent with previous experiments on CENPN downregulation., but neither migration nor invasion were significantly affected. Western blot results revealed that the expression of p53, p27, p21, CDK4, cyclin D1, CDK2, cyclin E, pRb, E2F1 and c-myc decreased after CENPN knockdown, but there was no significant change in total Rb levels. In addition, CENPN-knockdown cells subjected to irradiation showed significantly enhanced of γ-H2AX expression and reduced colony formation.

Conclusion
CENPN functions as an oncogene in HCC and may be a therapeutic target and promising prognostic marker for HCC.
CHA2DS2-VASC Score as a Predictor of Coronary Artery Disease: A Cross Sectional Observational Study
Authors: Shehata Mohamed Magdy , Kassem Hanan Kamel , Ibrahim Ibtsam Khairat , Deraz Inas Elsayed 
Published: May 2021

Background: The CHA2Ds2-VASC was was revealed to be a predictor for thromboembolism event in patients who do not have atrial fibrillation or who have supra ventricular arrhythmia. The aim of this work was to evaluate the role of CHA2DS2 – VASC score in prediction of coronary artery disease.
Methods: This cross sectional observational study included 150 patients underwent coronary angiography for diagnosis and treatment of CAD. There were 59 patients with ACS (including STEMI and Non-STEMI) and 91 patients with no ACS. All patients were subjected to complete history taking, clinical, general examination and local cardiac examination. Standard 12-lead ECG was obtained within 10 minutes of first medical contact (FMC) according to ESC guidelines. Baseline laboratory tests were done including serum creatinine, INR, hemoglobin, platelets, cholesterol and triglycerides. Arterial coronary angiography (Femoral approach), right and left coronary imaging and echocardiographs were performed.
Results: The incidence of HTN, CHF, DM and Vascular disease was statistically significantly higher in the cases with ACS as compared with the cases with no ACS. The total mean Gensini score was significantly higher in the cases with ACS as compared with the cases with no ACS. There was a statistically significantly strong positive correlation between CHA2DS2‐VASc Score and Gensini score. The best cut-off point of CHA2DS2‐VASc Score to differentiate between cases with ACS and no ACS WAS > 2 with 79.7% sensitivity and 56% specificity. With univariate regression analysis, increasing age, CHF, DM, previous stroke, vascular disease and increasing CHADS-VASC score were reported as risk factors for vessel affection, however with multivariate regression analysis CHF, DM and increasing CHADS-VASC score were shown as independent risk factors of vessel affection
Conclusions: CHA2DS2-VASc score could be utilized as a useful diagnostic and predictor tool in cases with CAD. Patients with higher CHA2DS2-VASc scores had higher risks of cardiovascular disease severity.
Effect of Zinc Fertilization on Yield, Quality of Soybean and Zinc Pools in a Typic Haplusterts
Authors: Jain Sushmita , Tagore G. S. , Sharma B. L. 
Published: May 2021

Field experiment was conducted at the research Farm of JNKVV, Jabalpur (M.P) during the Kharif season of 2018 with three main treatments as single year application, alternate and each year zinc addition and five levels of Zn (0, 2.5, 5, 7.5 and 10 kg ha-1 as sub treatments in a split plot design. Results revealed that alternate year Zn application increased seed yield by 1.48 t ha-1 over single year. The difference recorded in between alternate year and single year Zn application was 0.33 t ha-1. Treatment 5.0 kg Zn ha-1 had significantly increased seed yield by 1.43 t ha-1 over control. The difference recorded in between 5.0 kg Zn ha-1 and in control Zn application was 0.38 t ha-1.
The each year Zn application and 10 kg Zn ha-1 gave the highest protein (40.30%) and oil content (20.17%) in seed. Results explained that the higher release of Zn pools were observed with the each year Zn application and 10 kg Zn ha-1. The order of prevalence of Water soluble and exchangeable-Zn< Complexed-Zn<Amorphous Sesquioxide < Crystalline Sesquioxide bound-Zn< Residual-Zn.
Knowledge, Attitudes and Practices of Primary Health Care Physicians with Regard to Laryngopharyngeal Reflux in the Qassim Region of Saudi Arabia
Authors: Alanazy Sultan Mohammed , Alawaji Rakan Ibrahim , Alsager Ahmad Hamad , Alasqah Abdulrahman Abdullah , Aljabr Ahmed Abdullah , Alwabili Mohammed Ahmed , Alsuhaibani Abdulazizabdullah , Aldehami Mohammad Abdulrahman 
Published: May 2021

Many physicians confuse laryngopharyngeal reflux disease (LPR) with gastroesophageal reflux disease (GERD), leading to misdiagnosis of these two diseases. LPR is a common condition among the population, and physicians should be aware of it to save time, effort, resources, and money. The key objective of the study is to assess the level of knowledge, attitudes, and practices of primary care physicians with respect to LPR and its management and presentation in the Qassim region of Saudi Arabia.
Methods: A quantitative observational cross-sectional study was conducted at the primary health care centers in the Qassim region of Saudi Arabia. The sample included willing respondents among the primary health care physicians in these health care centers. Written consent was obtained from each participant before including their data in the study. The data were tabulated and analyzed using SPSS version 25.
Results: The sample consisted of 109 clinicians from different specialties. The average age of the respondents was 44.3 years (standard deviation [SD]: 9.35 years, 95% confidence interval [CI]: 35.7, 52.9) with an average experience of 12.94 years (SD: 8.58 years, 95% CI: 11.3, 14.6). Forty-six percent (46.8%) of the respondents did not agree that there is an association between sinusitis and LPR. About (43.11%) of clinicians said that they always educate patients about LPR, compared to (24.77%) of the clinicians who said that they do not educate patients about LPR.
Conclusions: Clinicians in the Qassim region of Saudi Arabia have relatively good knowledge, attitudes, and practices with regard to LPR.
THE RISK OF TUBERCULOSIS INFECTION IN THE HOSPITAL ENVIRONMENT
Authors: Couto Ingrid Ramos Reis , Andrade Marilda , Flach Diana Mary Araújo de Melo , Couto Ivo Batista Reis 
Published: May 2021

Objetivos: Identificar na literatura evidências disponíveis sobre a ocorrência da tuberculose pulmonar nos profissionais de enfermagem e mecanismos de controle da tuberculose existentes na unidade hospitalar. Metodologia: Revisão integrativa nas bases de dados da LILACS, MEDLINE e banco de tese da USP, no período de 2000 a 2010. Resultados: Os profissionais que mais desenvolveram a tuberculose doença em seu ambiente de trabalho foram: enfermagem citada em 14 (82,4%) e medicina citados em 03 (17,6%) dos artigos analisados, em relação aos demais profissionais de saúde. Conclusão: É de extrema importância a implementação de medidas de biossegurança em unidades hospitalares, bem como a investigação da infecção da tuberculose pulmonar em profissionais de saúde.
SIGN LANGUAGE: HOW THE NURSING STAFF INTERACTS TO TAKE CARE OF DEAF PATIENTS?
Authors: Machado Wiliam César alves , Machado Daniel Aragão , Figueiredo Nébia Maria Almeida de , Tonini Teresa , Miranda Rodrigo Sousa de , Oliveira Gabriela Moraes Bueno de 
Published: May 2021

Objetivo: Identificar como profissionais da equipe de enfermagem de um hospital universitário interagem para cuidar de seus clientes surdos, considerando-se fundamental o domínio da Língua de Sinais Brasileira (Libras) para planejamento da assistência de enfermagem prestada a esta clientela. Método: Pesquisa descritiva, exploratória, com abordagem quanti-qualitativa, realizada no segundo semestre de 2011 com 37 profissionais da equipe de enfermagem de hospital universitário da rede federal de ensino localizado na cidade do Rio de Janeiro. Os elementos quantitativos foram analisados a luz da estatística descritiva, elucidando o perfil sociodemográfico dos sujeitos (sexo, idade, faixa etária, categoria profissional, tempo de atuação profissional e domínio de Libras), enquanto os dados qualitativos foram extraídos das informações referentes à interação ao cuidar de clientes surdos foram analisadas a luz da técnica de análise de conteúdo. Resultados: Nenhum profissional da equipe domina Libras e 21 (57%) informaram nunca ter prestado cuidados a clientes surdos. Das estratégias de comunicação utilizadas pelos 16 (43%) dos profissionais de enfermagem que já prestaram cuidados aos clientes surdos destacaram-se: 12 (46,15%) referências ao uso da mímica; 4 (15,38%) menções ao uso da leitura labial; 8 (30,77%) referências ao uso da escrita; 1 (3,85%) referência ao uso do desenho; e 1 (3,85%) menção à ajuda de intérprete para se comunicar com clientes surdos quando lhes prestaram cuidados de enfermagem. Conclusão: Conclui-se que é preciso a tomada de providências efetivas para que profissionais de enfermagem se comuniquem adequadamente com os clientes surdos, a começar pela oferta regular de disciplinas específicas em todos os cursos e programas de ensino, habilitando os estudantes e, consequentemente, as futuras gerações para que possam planejar a assistência e prestar cuidados dignos a essas pessoas. Descritores: Cuidado de Enfermagem; Surdez; Acessibilidade; Língua de sinais; Pessoas com deficiência.
UNVEILED THE LARVAL THERAPY AS ALTERNATIVE IN THE TREATMENT OF SKIN LESIONS: AN INTEGRATIVE REVIEW
Authors: Da Silva Kauana Flores , Marchiori Mara Regina Caino Teixeira 
Published: May 2021

Objetivo: identificar nas evidências científicas da literatura quais os benefícios que a terapia larval proporciona aos pacientes com lesões de pele através de uma revisão sistemática da literatura, na modalidade de revisão integrativa. Método: para a busca dos artigos e a análise dos dados, foram utilizados dois instrumentos de avaliação. A pesquisa foi realizada entre julho de 2009 a julho de 2010. Resultado: a terapia larval mostrou-se positiva na maior parte dos estudos selecionados tanto em relação à restauração rápida dos tecidos como nos custos. Conclusão: os resultados apresentados proporcionaram um maior conhecimento sobre a terapia larval e mostraram que, apesar de não haver indícios de seu uso no Brasil, pode ser uma opção eficaz, principalmente quando os tratamentos convencionais não funcionam mais. Descritores: Larva; Moscas; Cicatrização de Feridas.
THE PROFILE OF PARTICIPANT WOMEN IN A NURSING EXTENSION PROGRAM
Authors: Valente Geilsa Soraia Cavalcanti , Lindolpho Mirian da Costa , De Mello Liliane Pinheiro , Gomes Helena Ferraz , Sá Selma Petra Chaves 
Published: May 2021

Objetivo: Identificar o perfil das mulheres participantes do Programa de Extensão A enfermagem na Atenção à saúde do idoso e seus cuidadores (EASIC). Método: Pesquisa documental com abordagem quantitativa dos dados, realizada entre os meses de junho a julho de 2010, no EASIC. Pesquisa aprovada no comitê de ética da Faculdade de Medicina da Universidade Federal Fluminense – UFF sob o n° 118/2010. O tratamento dos dados se deu por frequência percentual simples. Resultados: das 458 mulheres atendidas no EASIC; 44,1% tem idade entre 71 a 80 anos; 30,1% são casadas;16,2% recebem 1 salário mínimo; 36,7% possuem como escolaridade o ensino fundamental incompleto; 20,1% são da religião católica; 53,9% reside em Niterói; e 63,1% possuem associações de várias patologias. Conclusão: O conhecimento do perfil da clientela a ser atendida é essencial para identificar as demandas e necessidades de saúde, respeitando os saberes e preservando a resiliência desta população.