bluuebunny/crossref_metadata_2025_split huggingface dataset:
Scalp Acupuncture Protects Against Neuronal Ferroptosis by Activating the P62-Keap1-Nrf2 Pathway in ICH Rat Models
Authors: Li Ming yue , Dai Xiaohong , Yu Xueping , Zou Wei , Teng Wei , Liu Peng , Yu Xinyang , An Qi , Wen Xin 
Published: April 2021

Abstract
Intracerebral hemorrhage (ICH) is a devastating disease and different patients have varying degrees of neurological impairment despite after treatment. However, the therapeutic strategies targeting currently known cell death pathways of the secondary injury fail to effectively improve neurological deficits after ICH. As a new form of cell death, ferroptosis is closely related to neurological deficits after ICH. Moreover, increasing evidence has shown that scalp acupuncture (SA) has a neuroprotective effect on ICH rats by mediating different molecular pathways. Our study sought to investigate the effect of SA treatment on neurological function and elucidate whether SA could alleviate secondary injury after ICH by regulating ferroptosis, alongside analyzing its underlying mechanisms. First, our results demonstrated that SA treatment (Baihui [DU20] acupoint-penetrating-Qubin [GB7] acupoint, on the lesion side) could attenuate neurological functional impairment in ICH rats, according to the Ludmila Belayev test, and improve pathological injury according to hematoxylin-eosin staining. Second, perihematomal brain tissues from different groups were collected to detect the biological characteristics of neuronal ferroptosis including iron overload, lipid peroxidation accumulation, mitochondrial morphology changes and the decreased NeuN expression levels. Results revealed that SA treatment could attenuate neuronal ferroptosis in ICH rats to a remarkable degree. Furthermore, consistent with deferoxamine (DFX) being one of the main inhibitors of ferroptosis, there were no significant differences between the effects of SA treatment and DFX administration. The molecular mechanism studies found that SA treatment triggered the antioxidant effect alongside activating the p62/Keap1/Nrf2 pathway and up-regulating levels of ferritin heavy chain 1 (FTH1) and glutathione peroxidase 4 (GPX4), which contributed to reduced iron overload and oxidative stress in ICH rats. These results suggest that the p62/Keap1/Nrf2 signaling pathway is responsible for alleviating ferroptosis lipid peroxidation injury after ICH, thereby providing new theoretical basis with regards to SA treatment of ICH.
Analysis of the Effect of DRG Medical Insurance Payment on the Performance of Hospital Service in Hunan Province
Authors: Wang Yi-ting , Lu Xiao-qin , Peng Lin-lin , Chen Qiong , Wang Jian-qiang , Zhang Fu-feng 
Published: April 2021

Abstract
Background: Performance evaluation is an important means to promote the hospital's medical ability and management ability. Therefore, the aim of this study was to explore the effect of DRG payment mode on the performance of inpatient services in large tertiary hospitals. Methods: Based on the relevant report data of DRG pilot hospital and control hospital from 2015 to 2020, CN-DRG was used as grouping method, and case mix index (CMI), DRG group number, cost consumption index, time consumption index, low-risk group mortality and other indicators were used to analyze the impact of DRG on hospital inpatient service performance from the aspects of medical service ability, medical service efficiency, medical safety. Results: The average annual growth rate of DRG group was 4.07%, the average annual growth rate of CMI was 0.95%, the time consumption index and cost consumption index were lower than 1, and the mortality rate of low-risk group decreased by 27.56%. Conclusions: DRG medical insurance payment can improve the performance of inpatient service in the process of hospital management.
High Fibular Osteotomy Ameliorates Medial Compartment Knee Osteoarthritis in a Rabbit Model
Authors: Yan Feihua , Zhao Xujun , Duan Shisheng , Maimaiti Aini , Qi Yong , Li Maozhao , Maimaiti Muteli , Li Wenqiang 
Published: April 2021

Abstract
Purpose Knee osteoarthritis (KOA) is a common and severe disease characterized by articular cartilage degeneration, subchondral bone remodeling and inflammation. This study aimed to investigate the therapeutic effects of high fibular osteotomy (HFO) in a KOA rabbit model and to examine the molecular mechanisms involved in medial compartment KOA protective effects.Methods A rabbit model of destabilization of the medial meniscus was used to induce post-traumatic KOA. The effectiveness of HFO on protection against KOA was tested. Hematoxylin and eosin staining, Safranin O/Fast green staining and micro-CT analysis were performed to evaluate structural and morphological changes. The expression of metalloproteinase (MMP)-1, MMP-3, MMP-13, collagen type II (Col2), a disintegrin and metalloproteinase domain with thrombospondin motifs (ADAMTS)-5, aggrecan, interleukin (IL)-1β, IL-6 and tumor necrosis factor (TNF)-α was assessed by real time PCR, western blotting and enzyme-linked immunosorbent assay. Additionally, western blotting was performed to test the expression of NFκB p65, phospho-IκBα and IκBα. Results HFO delayed the progression of articular cartilage damage and suppressed subchondral bone remodeling. HFO also decreased MMP-1, MMP-3, MMP-13 and ADAMTS-5 expression, and increased Col2 and aggrecan expression. In parallel, HFO attenuated the expression of IL-1β, IL-6 and TNF-α. Furthermore, the molecular mechanism underlying the protective effect of HFO in medial compartment KOA was related to the NFκB signaling pathway. Conclusion HFO may be a novel therapeutic approach to treating medial compartment KOA.
Association Between Plasma Level of Superoxide Dismutase and Survival of Patients with Acute-On-Chronic Liver Failure
Authors: Tian Zhen , Yao Naijuan , Wu Yuchao , Wang Fei , Zhao Yingren 
Published: April 2021

Abstract
Fewer than 50% patients with acute-on-chronic liver failure (ACLF) recover spontaneously, and ACLF patients have high mortality without liver transplantation. Oxidative stress has been shown to mediate hepatic inflammation during liver failure. The aim of this study is to investigate whether a biomarker of oxidative stress exists in assessing severity and outcomes of patients with ACLF. Between January 2015 and September 2018, a retrospective cohort of 124 ACLF patients, together with healthy individuals, liver cirrhosis and acute liver failure (ALF) patients were analyzed. Plasma Superoxide Dismutase (SOD) level was measured using a commercial ELISA kit, survival analysis was carried out using the Kaplan-Meier method. Results indicated that patients with ACLF had statistically higher plasma SOD levels compared to controls (healthy controls and cirrhosis patients). A level of SOD > 428 U/mL was associated with a statistically increase in risk for mortality or liver transplantation due to ACLF. Combination of plasma SOD level and MELD score had improved performance in assessing severity and outcomes of ACLF. In conclusion, this research revealed that plasma SOD level measured at hospital admission can be used to assign patients with ACLF into high- and low-risk groups. Combination of plasm SOD level and MELD score was more closely associated with patient outcome than either value alone. This system might be used to determine patient prognoses and prioritize patients for liver transplantation.
Revealing the Tomato Endophyte Bacteria Communities Under Long-term Organic and Conventional Agricultural Practice System
Authors: Zhang Zeyu , Zhan Yabin , Zhang Zengqiang , Liu Youzhou , Xu Ting , Wei Yuquan , Li Ji 
Published: April 2021

Abstract
Purpose: Microorganisms associated with the plant can contribute to plant health. Discrepancy both soil microbial structure and physicochemical properties observed in the organic and conventional agricultural systems. However, very little is known about the diversity of endophytic bacterial communities in plants grown in separate manipulation systems. The goal of this work was to get a broader overview of the diversity and dynamics of the tomato endophytic bacteria in the different agricultural practice systems.Methods: The structure of tomato endophyte bacterial communities under different growing stage (seeding stage, flowering stage, fruiting stage and harvesting stage), agricultural manipulation practices (organic and conventional systems) and organ-type (root or stem) were explored by using 16S rRNA gene profiling in this study.Results: A total of 2,014,992 16S rRNA gene sequences were obtained. These sequences revealed large-scale functional taxonomy units (OTUs). That is, there are 648 different OTUs in libraries, and 96 OTUs are common. Tomato endophyte bacteria consisted mainly of four phyllum, of which Proteobacteria was the most represented, followed by Firmcutes, Bacteroides, Actinobacteria, Gamma proteobacteria, the most abundant class are proteobacteria, bacteriobacteria, and so on. Proteobacteria are low. Enterobacteriaceae, Vecella, Bacillus, Mesorizobium and Chrysobacterium were shared by all plant development stage. Rich endophytic bacterial diversity was observed at the seedling stage（TI）, and endophytic bacterial diversity at the flowering stage （T2）and fruiting stage （T4）was low. Significant difference in endophytic bacterial communities emerged from roots and different host biographical stages, and tomato exerts greater influence on endophyte bacteria compared to organ type (main) agricultural manipulation methods.Conclusions: Tomato endophyte microbiota have a distinct structure in different plant development stage. Bacillus.spp were enriched seeding stage (TI) and decreased in the fruiting stage while Mesorhizobium.spp increased during in the fruiting stage(T4). Tomato have distinct endosphere microbiota by comparing beta diversity of microbiota in all crop season, compared with the manipulation resume and organ niche. And a strong correlation was observed between the structure of the microbiota in the whole dataset and soil chemistry which indicated that the soil type and treatment affected the endosphere microbiota of tomato. Organ niche exert more influence on the tomato microbiota compared with agricultural treatments between organic-farming and conventional farming.
Associations between glucose metabolism disorders and prognosis in patients with acute-on-chronic liver failure
Authors: Hu Han , Hu Xinxin , Tian Caiyun , Zhu Yanping , Liu Yujuan , Cheng Qijiao , Yang Fangwan , Liu Jun , Li Ying , De Lin Shi 
Published: April 2021

Abstract
Background/Purpose Associations between glucose metabolism disorders and prognosis in patients with acute-on-chronic liver failure (ACLF) remain unclear. This study was conducted to investigate the clinical characteristics of glucose metabolism disorders and their associations with 90-day mortality in patients with ACLF. Methods Ninety-six patients with hepatitis B virus (HBV)-related ACLF without pre-existing diabetes were retrospectively included. Glucose metabolism disorders were diagnosed based on fasting plasma glucose and oral glucose tolerance test results on admission and during follow-up. Multivariate Cox proportional hazards analysis was used to identify independent risk factors for death within 90 days after admission. Results Among 96 patients with ACLF, 51 (53.1%) had diabetes, 29 (30.2%) had impaired glucose tolerance (IGT), and 17 (17.7%) had hypoglycemia. Patients with diabetes had significantly lower levels of homeostasis model assessment 2-β-cell function than did patients with normal glucose tolerance. Of 22 patients with diabetes or IGT and without antihyperglycemic treatment, 8 (36.4%) exhibited regression of their glucose metabolism disorders after a follow-up of 32.8 ± 28.8 days, and higher platelet levels were associated with regression. Twenty-five patients (25.0%) with ACLF died of liver failure within 90 days. Diabetes (odds ratio [OR] = 3.601, 95% confidence interval [CI]: 1.342–9.661) and age (OR = 1.045, 95% CI: 1.010–1.082) were the independent risk factors associated with mortality. Conclusions Impaired pancreatic β-cell function is related to diabetes development, and diabetes is associated with high mortality in patients with chronic HBV-related ACLF.
Fibromyalgia-Associated Hyperalgesia and Psychopathological Alterations are Unrelated to Gut Microbiome Diversity
Authors: Weber Thomas , Tatzl Eva , Kashofer Karl , Holter Magdalena , Trajanoski Slave , Berghold Andrea , Heinemann Akos , Holzer Peter , Herbert Michael Karl 
Published: April 2021

Abstract
Fibromyalgia-syndrome (FMS) is a complex disease characterized by chronic widespread pain and additional symptoms including depression, cognitive dysfunction (“fibro-fog”) and maldigestion. We examined whether FMS-related pain parameters assessed by quantitative sensory testing (QST) are related to changes of microbial diversity.We recruited 25 patients with FMS and 26 age- and sex-matched healthy controls. Medical background, food habits, psychopathology and quality of life were assessed with questionnaires. Stool samples were analyzed by 16S rRNA gene amplification and sequencing. QST was performed according to the protocol of the German Network for Neuropathic Pain.QST showed that both lemniscal and spinothalamic afferent pathways are altered in FMS patients relative to healthy controls and that peripheral as well as central pain sensitization processes are manifest. Psychometric assessment revealed enhanced scores of depression, anxiety and stress. In contrast, neither the composition nor the alpha- and beta-diversity of the fecal microbiome was changed in FMS patients.FMS patients segregate from healthy controls in various parameters of QST and psychopathology, but not in terms of composition and diversity of the fecal microbiome. Despite consideration of several confounding factors we conclude that the impact of the gut microbiome on the pathophysiology of FMS is limited.
Optimized Soft Frame Design of Traditional Printing and Dyeing Process in Xiangxi Based on Pattern Mining and Edge-Driven Scene Understanding
Authors: Xiao Hong 
Published: April 2021

Abstract
Traditional folk crafts are handicrafts and corresponding activities created by Chinese working people in all ages to meet their material and spiritual needs by using various material materials and handicrafts. It reflects the general characteristics of Arts and crafts, but also shows the distinctive personality of national culture. The folk printing and dyeing process of Miao Nationality in Xiangxi and Southeast Guizhou has this characteristic, which once brought a lot of convenience to people's life and became an important part of people's life at that time. However, under the impact of economic globalization, the diversity of human culture and the intangible cultural heritage of all ethnic groups are facing serious challenges. With the development of economy, the traditional manual printing and dyeing technology is gradually replaced by modern printing and dyeing technology, and its position in textile printing and dyeing is greatly reduced. However, it can not be ignored that the manual printing and dyeing process is still full of vitality, which is welcomed and loved by the public in the optimization design of fashion textiles. Traditional art carries a lot of spiritual things in its material form, so it has high aesthetic value, cultural value and profound spiritual connotation. Therefore, the optimization design of traditional printing and dyeing process framework in Xiangxi is worth studying. In this paper, using big data pattern mining algorithm and scene understanding algorithm, a framework optimization system of traditional printing and dyeing process in Xiangxi is constructed.
Anxiety and Depression in School-age Patients With Spinal Muscular Atrophy: a Cross-sectional Study
Authors: Yao Mei , Xia Yu , Feng Yijie , Ma Ying , Hong Yi , Zhang Yanyi , Chen Jie , Yuan Changzheng , Mao Shanshan 
Published: April 2021

Abstract
Background: Spinal muscular atrophy (SMA) is a rare neurogenetic disease which involves multisystem dysfunctions such as respiratory, digestive, and motor disorders. Anxiety, depression and other psychological disorders often accompany severe chronic physical diseases. The aim of this study was to investigate the prevalence of anxiety and depression along with their influencing factors among school-age patients with SMA.Methods: We conducted a cross-sectional study on school-age SMA patients in China. Patients aged 8–18 years with a genetic diagnosis of 5qSMA were invited to answer a questionnaire composed of sociodemographic and clinical questions, then to complete the Screen for Child Anxiety-Related Emotional Disorders and Depression Self-Rating Scale for depression and anxiety level evaluation. At the end of the questionnaire, further questions assessed the subjective anxiety and subjective depression of patients’ caregivers and their expectations for their child’s future. Results: Complete data were available for 155 patients. The sample included 45.8% boys and 54.2% girls; 65.2% were type II, 27.1% were type III, and the remainder were type I SMA. Rates of anxiety and depression in these school-age SMA patients were 40.0% and 25.2%, respectively. Gender, age, and disease type were not associated with anxiety or depression, but respiratory system dysfunction, digestive system dysfunction, skeletal deformity, rehabilitation exercise, academic delay, specialized support from school, household income level, caregivers’ subjective anxiety, and caregivers’ expectations were significantly related to both anxiety and depression. Conclusions: There was a high prevalence of anxiety and depression in school-age SMA patients in China. Professional psychological care maybe included in the standard of care. These results also call for possible targets for intervention such as reducing complications, improving drug accessibility, retaining normal schooling, strengthening school support, and enhancing the ability of the caregivers of SMA patients to assist in the diagnosis and treatment of the disease, so improving the mental health of SMA patients.
Nonholonomic Dynamics of the Twistcar Vehicle: Asymptotic Analysis and Hybrid Dynamics of Frictional Skidding
Authors: Or Yizhar , Halvani Oriel 
Published: April 2021

Abstract
The Twistcar vehicle is a classic example of a nonholonomic dynamical system. The vehicle model consists of two rigid links connected by an actuated rotary joint and supported by wheeled axles, where nonholonomic constraints are assumed to impose no skidding of the wheels. Recent experimental measurements conducted with a robotic Twistcar prototype have shown disagreements with previous theoretical analyses. In particular, significant skidding has been observed, in addition to discrepancies with respect to theoretical predictions of divergence in oscillations of the vehicle’s speed and orientation, as well as direction reversal depending on the vehicle’s structure.

The goal of our research is to resolve this disagreement by generalizing the theoretical analysis. First, we extend previous asymptotic analysis by incorporating the effects of links’ inertia and oscillation amplitude of the input angle on the direction of net motion. Next, we formulate the vehicle’s hybrid dynamics under frictional bounds and skid-state transitions. Using numerical analysis, we obtain optimal values for the vehicle’s mean speed and energetic cost-of-transport as a function of the input frequency. Our results improve the agreement between theory and experiments and suggest directions for further experimental investigation.
Prediction of H3K27M-mutant Brainstem Glioma by Amide Proton Transfer Weighted Imaging and Its Derived Radiomics
Authors: Zhuo Zhizheng , Qu Liying , Zhang Peng , Duan Yunyun , Cheng Dan , Xu Xiaolu , Sun Ting , Ding Jinli , Xie Cong , Liu Xing , Haller Sven , Barkhof Frederik , Zhang Liwei , Liu Yaou 
Published: April 2021

Abstract
Purpose H3K27M-mutant associated brainstem glioma (BSG) carries a very poor prognosis. We aimed to predict H3K27M mutation status by amide proton transfer weighted (APTw) imaging and radiomic features. Methods Eighty-one BSG patients with APTw imaging at 3T MRI and known H3K27M status were retrospectively studied. APTw values (mean, median and max) and radiomic features within manually delineated 3D tumor masks were extracted. Comparison of APTw measures between H3K27M-mutant and wildtype groups was conducted by two-sample Student’s T/Mann-Whitney U test and receiver operating characteristic curve (ROC) analysis. H3K27M-mutant prediction using APTw-derived radiomics was conducted using a machine-learning algorithm in randomly selected train (n=64) and test (n=17) sets. Sensitivity analysis with additional random splits of train and test sets, 2D tumor masks and other classifiers were conducted. Finally, a prospective cohort including 29 BSG patients was acquired for validation of the radiomics algorithm. Results BSG patients with H3K27M-mutant were younger and had higher max APTw values than those with wildtype. APTw-derived radiomic measures reflecting tumor heterogeneity could predict H3K27M mutation status with an accuracy of 0.88, sensitivity of 0.92 and specificity of 0.80 in the test set. Sensitivity analysis confirmed the predictive ability (accuracy range: 0.71-0.94). In the independent prospective validation cohort, the algorithm reached an accuracy of 0.86, sensitivity of 0.88 and specificity of 0.85 for predicting H3K27M-mutation status. Conclusion BSG patients with H3K27M-mutant had higher max APTw values than those with wildtype. APTw-derived radiomics could accurately predict a H3K27M-mutant status in BSG patients.
Identification of Key Biomarkers Associated with Survival and Prognosis in Colon Adenocarcinoma
Authors: Zhou Yanjie , Jiang Lu , Lin Jiang , Tang Wendong , Jiang Wenqian , Wang Ke 
Published: April 2021

Abstract
Background: Colorectal cancer (CRC) has a high rate of relapse and recurrence that result in poor prognosis and unsatisfactory outcomes. Colon adenocarcinoma (COAD) is the most prevalent type of CRC. It is crucial to identify novel molecular biomarkers for the early diagnosis, prognosis evaluation and disease monitoring of COAD.Methods: Three gene expression profile data were downloaded from the Gene Expression Omnibus(GEO), and the differential expression genes(DEGs) were identified by GEO2R. Gene Ontology (GO) and KEGG pathway enrichment analysis were conducted by WebGestalt online tool. String database and Cytoscape software were used for protein–protein interaction (PPI) network construction and module analysis. The top 20 Hub Genes were screened from the PPI network using MCC algorithm on CytoHubba app of Cytoscape software, and were verified by ONCOMINE database then. The core genes affecting CRC prognosis were screened by GEPIA2 survival analysis web tool. Finally, the expression level and clinical indicators including core genes was analyzed by TCGA-COAD dataset.Results: In total, 413 differentially expressed genes (DEGs) were identified, and the GO and KEGG enrichment analyses of DEGs were processed. After, the protein–protein interaction (PPI) network was constructed and 20 hub genes were identified. Furthermore, three core genes were selected via survival analysis . Finally, the diagnostic and prognostic value of these core genes was verified by clinical analysis of TCGA-COAD dataset.Conclusion: SPP1, GRP and GNGT1 were all over-expressed in COAD, and may be regarded as novel diagnostic and prognostic biomarkers for COAD.