bluuebunny/crossref_metadata_2025_split huggingface dataset:
Tannic Acid Induces Apoptosis In Bladder Cancer Cells Via Mitochondrial-Dependent And AKT Pathway
Authors: Chen Ming-Cheng , S Ann Seles , Velmurugan Bharath Kumar , Hsu Chiung-Hung , Day Cecilia Hsuan , Chen Ray-Jade , Wang Tso-Fu , Viswanadha Vijaya Padma , Li Chi-Cheng , Huang Chih-Yang 
Published: September 2021

Abstract
Urothelial bladder cancer is rapidly spreading across Western countries, and therapy has shown little-to-moderate effects on bladder cancer. Thus, focusing on curbing cancer incidence has become crucial. In this study, we examined the effects of tannic acid, a polyphenol with anti-fungal and anti-cancer effects, on UMUC3 bladder cancer cells. UMUC3 cells were exposed to tannic acid for 24 hours, following which we observed significant inhibition of cell proliferation. Additionally, flow cytometry and TUNEL assay revealed that tannic acid induces apoptosis in UMUC3 cells in a dose-dependent manner. Furthermore, tannic acid treatment upregulated the expression of cleaved caspase-3 which further confirmed induction of apoptosis in UMUC3 cells by treatment with tannic acid. TA treatment decreased stem cell markers expression such as SOX2, OCT4, and NANOG. Together, these results showed that tannic acid can promote apoptosis; therefore, it may have potential as treatment for bladder cancer.
Enhanced Photocatalytic Activity of Two Dimensional SrTiO3 Nano Structures for Dye Degradation
Authors: AlIssa Y. , Sajjadian S. T. , Hamidi S. M. , Sadeghi S. , Nabid M. R. 
Published: September 2021

Abstract
The combination of the properties of nanostructures, photocatalytic semiconductors, and plasmonic materials can significantly contribute to improving the performance of the entire photocatalysis process. We tried to produce a unique catalyst by combining these three properties. We have produced a two-dimensional nanostructure which was then coated with gold as a plasmonic material followed by SrTiO3 as semiconductor material. The result was the production of the first catalyst of its kind made in this way, which offered notable results. The degradation ability of the sample was examined with different degrees of pH of rhodamine B dye, where we achieved the highest degradation, 16%, at pH10.
Effects of Bile Acids Supplement in High Plant Protein Diet on Common Carp (Cyprinus Carpio) Bile Acids Profile and Hepatopancreas Health
Authors: Xian Wei , Ting Yao , FALL Fatou Ndoye , Xiaofang Liang , Jie Wang , Wenlong Du , Xu Gu , Min Xue 
Published: September 2021

Abstract
BackgroundBile acids (BAs) have considerable importance in the metabolism of glycolipid and cholesterol. BAs profile in mammals has been widely reported, but lacking in fishes. The purpose of the present study is to clarify BAs profiles of common carp and how exogenous additions of BAs could alleviate hepatopancreas injured of common carp under a high plant protein diet. A 11-week feeding trial was conducted with high plant protein diet (18%) (HP) and high plant protein diet (18%) added 600 mg/kg BAs (HP+BAs) for Common carp, and then the UHPLC-MS/MS technology was used to analyze the BAs in the bile and plasma of two groups. ResultsHP could induce vacuolation of hepatocytes and accumulation of glycogen in Common carp, while these phenotypes were significantly improved in HP+BAs group. In addition, BAs profile of HP group and HP+BAs group were described in detail, for Common carp bile with treatment by exogenous BAs, TCA, CA, T β MCA and T ω MCA were the main components. Furthermore, in HP+BAs group plasma, CDCA, CA, LCA, and GCDCA increased significantly, they could activate TGR5, the activation of hepatopancreas TGR5 might regulate glucose metabolism to relieve hepatopancreas glycogen accumulation. ConclusionsHP could induce glycogen accumulation in common carp hepatopancreas while supplemented BAs to HP could mitigate this symptom. And we determined that the reduction of common carp hepatopancreas glycogen accumulation in the HP+BAs group is importantly related to the change in the endogenous BAs profile after the addition of BAs in HP through an integrated bile acids profile determination by UHPLC-MS/MS . This study has important guiding significance for aquaculture.
Effect of Kinesio Taping on Low Back Pain During Pregnancy: A Protocol for Systematic Review and Meta-Analysis
Authors: Xue Xiali , Yang Xinwei , Tu Huan , Deng Zhongyi , Chen Yan , Mao Xiaorong , Li Ning 
Published: September 2021

Abstract
Background: Low back pain during pregnancy can affect the daily living activities of pregnant women to a certain extent, and even lead to fetal agitation and threatened abortion. Kinesio taping (KT) can improve tissue circulation and provide elastic supports, which is a reliable method to treat low back pain. At present, there is a lack of high-level clinical evidence for the treatment of low back pain during pregnancy with KT. Therefore, this study will systematically review and analyze currently available randomized controlled trials to evaluate the efficacy and safety of KT in the treatment of low back pain during pregnancy.Methods and analysis: This protocol is guided by the Preferred Reporting Items for Systematic Reviews and Meta-Analyses Protocols. We will search the following database sources of the Randomized controlled trials: PubMed, the Cochrane Library, EMBASE, Web of Science, Chinese Biomedical Literature Database (CBM), Chinese National Knowledge Infrastructure Database (CNKI), Chinese Science, and the Wanfang Database. From the establishment of the database to April 2021. Two independent investigators will conduct an electronic literature search, study selection, data extraction, and quality assessment to summarize and evaluate the efficacy of KT in the treatment of low back pain during pregnancy. and risk of bias will be assessed using the Cochrane bias risk tool. All data analysis will be conducted using Revman5.3 software.Result: This study will objectively and comprehensively evaluate the efficacy and safety of randomized controlled trials of KT for low back pain during pregnancy, and submit the results to a peer-reviewed journal for publication.Conclusion: This study will provide clinicians with the latest high-quality evidence for the use of KT in the treatment of low back pain during pregnancy.Systematic review registration: PROSPERO CRD42021250373
Cohort Profile: Norwegian Survey of Health and Ageing (NORSE)
Authors: Strand Bjørn Heine , Skirbekk Vegard , Langballe Ellen Melbye , Bergh Sverre , Landmark Brynjar , Wangensteen Sigrid , Selbæk Geir , Kirkevold Øyvind 
Published: September 2021

Abstract
Purpose: The Norwegian Survey of Health and Ageing (NORSE) was set up to provide internationally comparable data on ageing in Norway, which includes measured intrinsic capacity and cognitive function.Participants: NORSE is a population-based health examination study of seniors aged 60+ from the 1929-1959 birth cohorts in the former Norwegian county of Oppland, interviewed and examined in three study waves 2017-19 (N=957, 16% response rate). NORSE is based on the SHARE (share-project.org) questionnaire, which includes work-related information, self-assessed and retrospective health, and expectations on longevity, quality of life, volunteering activities, consumption, and financial arrangements. In addition, several objective measures of intrinsic and cognitive capacity are included.Findings to date: A shorter preferred life expectancy (PLE)was found to be associated with the prospects of a life with dementia and chronic pain.Motivation for retirementwas found to be related towork-life experience and health.Social media was mostly used in the younger age groups and there was a tendency towards more use in the higher educational groups. NORSE incorporates questions on religion, and older women tend to have a higher degree of religiosity (proxied as self-assessed religiosity) than men in their 80s, but more similar (and lower levels) among those in their 60s.Future plans: NORSE participants have allowed their data to be linked to National registry data and midlife health examination studies and thereby provide a longitudinal design as well as information on disability status, socioeconomic status, household and marital status, support to/from children and parents, and pension status.
The Efficacy and Tolerability of Sitagliptin, Saxagliptin, Vildagliptin, Lindagliptin, Alogliptin, Omarigliptin and Trelagliptin in Patients with Type 2 Diabetes: Protocol for a Network Meta-Analysis of Randomized Control Trials
Authors: chen guoquan , Chen Jiale , Hu Xiaoxia , Chen Jianqing , He Yiling 
Published: September 2021

Abstract
Backgound: Type 2 diabetes mellitus remains a major public health challenge in the worldwide. The control of blood glucose is essential in the metabolic management of diabetes. Dipeptidyl peptidase-4 (DPP-4) inhibitors are new hypoglycemic agents with good hypoglycemic effect. However, the difference of efficacy and tolerability among different kinds of DPP-4 inhibitors remains unclear and no network meta-analysis(NMA) has been performed yet to compare different kinds of DPP-4 inhibitors comprehensively. Our research aims to investigate and rank different kinds of DPP-4 inhibitors on efficacy and tolerability.Methods/design: English language studies will be searched in the electronic databases(PubMed, the Cochrane Library and EMBASE), without time restriction. Parallel-group randomized controlled trials (RCTs) meeting the following criteria will be included, regardless of the blinding design: (1) T2DM patients aged over 18, pregnant or breastfeeding female patients and patients with T2DM on Maintenance Hemodialysis will be excluded, (2) interventions with DPP-4 inhibitors (sitagliptin, saxagliptin, vildagliptin, lindagliptin, alogliptin, omarigliptin and trelagliptin), traditional antidiabetic agents includes α-glucosidase inhibitors, sulphonylureas, non-sulfonylureas and thiazolidinediones, or placebo and (3) change in HbA1c concentration, weight and FPG from baseline to the end of treatment and proportions of patients in achieving HbA1c < 7.0%, patients with treatment-emergent adverse events leading to treatment discontinuation and patients with hypoglycaemia. Random effects pairwise and Bayesian NMA will be performed for these outcomes. Subgroup analyses are carried out for race and dosing frequency.Discussion: Our NMA will be the first to systematically summarize, compare and rank the efficacy and tolerability of different kinds of DPP-4 inhibitors in T2DM patients. We are confident that our research project will significantly contribute to optimizing hypoglycemic therapy.Systematic review registration:PROSPERO:CRD42021253822
Preparation for Animal Models of Osteoporosis: a Systematic Review
Authors: Mu Panyun , Qu Peihua , Li Yulin , Feng Jie , Ma Xu , Xiong Feng , Hu Yimei 
Published: September 2021

Abstract
Background: it is of great significance for clinical diagnosis, prevention and treatment of osteoporosis to deeply understand the pathogenesis and development process of osteoporosis through animal models of osteoporosis. This systematic review aim to summarize the modeling methods of osteoporosis, reveal the current situation and progress of animal models of osteoporosis, and compare the advantages and disadvantages of various modeling methods, so as to provide reference for clinical research.Methods: CNKI, CBM database, VIP database, Wanfang database, PubMed database and EMBASE database were searched by computer from the database establishment to December 2020 with the key words of "animal model; osteoporosis" in Chinese and English respectively. The literatures were screened according to inclusion and exclusion criteria. The methods of osteoporosis modeling, the improvement of the methods and the advantages and disadvantages of each method are summarized.Discussion: a total of 9303 related literatures were collected, and 112 eligible literatures were included. The establishment of an appropriate animal model is the key to the etiology, pathophysiology and drug therapy of osteoporosis. As the causes and pathophysiological changes of different types of OP have their own characteristics, the modeling methods are also different. Therefore, different modeling methods and experimental animals should be selected according to different experimental requirements.Systematic review registration: No
Colon Cancer Classification and Prognosis Prediction Based on Genomics Multi-features
Authors: Xu Jiasheng , Liao Kaili , Wu Chengfeng , Yang Qijun , Wan Hongping , Wang Xiaozhong 
Published: September 2021

Abstract
Background: To classify colon cancer and predict the prognosis of patients with multiple characteristics of the genome.Methods: We used the mRNA expression profile data and mutation maf files of colon cancer patients in the TCGA database to calculate the TMB value of patients. Combined with CNV, MSI, and corresponding clinical information, the patients were clustered by the "K-means" method to identify different molecular subtypes of colon cancer. Comparing the differences of prognosis, and immune cell infiltration, and other indicators among patients in each subgroup, we used COX and lasso regression analysis to screen out the prognosis difference genes among subgroups and construct the prognosis prediction model. We used the external data set to verify the model, and carried out the hierarchical analysis of the model to compare the immune infiltration of patients in the high and low-risk groups. And detected the expression differences of core genes in tumor tissues of patients with different clinical stages by qPCR and immunohistochemistry.Results: We successfully calculated the TMB value and divided the patients into three subgroups. The prognosis of the second subgroup was significantly different from the other two groups. The mmunoinfiltration analysis showed that the expression of NK.cells.resting increased in cluster1 and cluster 3, and the expression of T.cells.CD4.memory.resting increased in cluster3. By analyzing the differences among subgroups, we screened out eight core genes related to prognoses, such as HYAL1, SPINK4, EREG, and successfully constructed a patient prognosis evaluation model. The test results of the external data set shows that the model can accurately predict the prognosis of patients; Compared with risk factors such as TNM stage and age, the risk score of the model has higher evaluation efficiency. The experimental results confirmed that the differential expression of eight core genes was basically consistent with the model evaluation results.Conclusion: Colon cancer patients were further divided into three subtypes by using genomic multi-features, and eight-core genes related to prognosis were screened out and the prognosis evaluation model was successfully constructed. With external data and experiments, it verified that the model had good evaluation efficiency.
Plant–Soil Feedback In Camellia Oleifera And Mixed Gardenia Jasminoides Ellis–Camellia Oleifera Stands Determined By Soil Bacterial Community Analysis
Authors: Wang Yun , Xie Peng , She Jiyun , Deng Aihua , Fan Shaogang 
Published: September 2021

Abstract
Purpose: Little is known regarding the combined impact of plant and soil traits on the soil bacterial community. Herein, we assessed physical and chemical properties along with bacterial community structure in soils sampled at different depths (0–20 cm, 20–40 cm, and 40–60 cm) and slope positions (peak, hillside, and bottom), in Camellia oleifera monoculture and mixed Gardenia jasminoides–Camellia oleifera stands. Methods: Soil physicochemical characteristics were determined using standard methods. The composition of soil bacterial communities was evaluated using high-throughput sequencing of the 16S rRNA gene. Results: Soil organic carbon, humus, and total organic contents were higher in G. jasminoides + C. oleifera low-yielding forest than in other stands, however, the NH4+-N levels were significantly lower than that in monoculture. The slope position did not greatly influence soil physical and chemical properties. The dominant bacteria were Proteobacteria, Chloroflexi, Acidobacteria, and Actinobacteria. The alpha and beta diversity and abundance of soil bacterial community were higher in intercropping systems than in monoculture systems. Potassium and nitrogen levels and pH significantly affected the soil microbial community composition. Correlation analysis revealed that alkaline hydrolysable nitrogen and pH were significantly correlated with the input of plant-associated organic matter and dynamic changes of keystone taxa.Conclusion: G. jasminoides improved the physicochemical characteristics of C. oleifera low-yielding soil and greatly affected the soil bacterial community, further improving the soil microecological environment. Therefore, this specific intercropping system is an effective strategy for improving soil health.
Bacteriophage EK99P-1 Alleviates Enterotoxigenic Escherichia Coli K99-Induced Barrier Dysfunction and Inflammation
Authors: Kim Narae , Gu Min jeong , Kye Yoon-Chul , Ju Young-Jun , Hong Rira , Ju Do Bin , Pyung Young Jin , Han Seung Hyun , Park Byung-Chul , Yun Cheol-Heui 
Published: September 2021

Abstract
Background
Bacteriophages have long been used as a potential alternative to antibiotics for livestock due to their ability to specifically kill enterotoxigenic Escherichia coli (ETEC), which are a major cause of diarrhea in piglets. However, the control of ETEC infection by phages within intestinal epithelial cells, and their relationship with host immune responses, remain poorly understood.
Results
In this study, we evaluated the effect of phage EK99P-1 against ETEC K99-infected porcine intestinal epithelial cell line (IPEC-J2). Phage EK99P-1 prevented ETEC K99-induced barrier disruption by attenuating the increased permeability mediated by the loss of tight junction proteins such as zonula occludens-1 (ZO-1), occludin, and claudin-3. ETEC K99-induced inflammatory responses, such as IL-8 secretion, were decreased by treatment with phage EK99P-1. We used a IPEC-J2/peripheral blood mononuclear cell (PBMC) Transwell co-culture system to investigate whether the modulation of barrier disruption and chemokine secretion by phage EK99P-1 in ETEC K99-infected IPEC-J2 would influence basolateral immune cells. The results showed that phage EK99P-1 reduced the mRNA expression of ETEC K99-induced pro-inflammatory cytokines, interleukin (IL)-1β and IL-8, from pPBMC collected on the basolateral side.
Conclusion
Together, these results suggest that phage EK99P-1 prevented ETEC K99-induced barrier dysfunction in IPEC-J2 and alleviated inflammation caused by ETEC K99 infection. Reinforcement of the intestinal barrier by phage EK99P-1 also modulates the immune cell inflammatory response.
Cognitive Strengths Assessment and Intervention in First Episode Psychosis: A Thematic Analysis of Clinicians’ Perspectives
Authors: Steele Peter , Cheng Nicholas , Phillips Lisa J. , Bryce Shayden , Alvarez-Jimenez Mario , Allott Kelly 
Published: September 2021

Abstract
Background

Cognitive deficits are associated with poor functional outcomes in individuals recovering from a first episode of psychosis (FEP). Existing treatments that target cognitive deficits in FEP may enhance cognitive function, but improvements to real-world functioning are less consistent. Furthermore, these treatments may not adequately address the personal recovery goals of young people attending FEP services. A novel cognitive strengths-based approach may overcome these shortcomings.
Methods

This qualitative study used semi-structured interviews to explore clinicians' (N = 12) perspectives toward the potential development of a cognitive strengths-based assessment or intervention in FEP. The interviews were analysed using thematic analysis.
Results

Five higher-order themes emerged: (1) pro-strengths attitude despite unfamiliarity and minimal use, (2) default to a cognitive deficit lens, (3) potential benefits of a cognitive strengths approach, (4) potential risks and barriers, and (5) considerations for successful implementation. While clinicians acknowledged their current deficit approach, they supported implementing a cognitive strengths assessment or intervention and highlighted their potential benefits for the personal recovery needs of young people with FEP.
Conclusions

These findings suggest that a deficit-focused approach to cognitive function amongst clinicians may be common practice in FEP services. Nevertheless, a cognitive strengths approach to assessment and intervention was viewed favourably by clinicians and may represent a novel method of supporting personal recovery. Thus, the design and implementation of a cognitive strengths approach may be worthwhile. Future exploration of other stakeholder perspectives, such as young people with FEP, is essential.
Distribution and Molecular Analysis of Subtilase Cytotoxin Gene (subAB) Variants in Shiga Toxin-Producing Escherichia Coli (STEC) Isolated From Different Sources in Iran
Authors: Badouei Mahdi Askari , Jajarmi Maziar , Narimani Aria , Salehi Taghi Zahraei , Ghanbarpour Reza , Morabito Stefano , Koochakzadeh Alireza 
Published: September 2021

Abstract
Background

Subtilase is a potent cytotoxin that was first described in O113:H21 strain in Australia as a plasmid- encoded cytotoxin (subAB1). Subsequently, chromosomal variants including subAB2-1, subAB2-2, and subAB2-3 were described.
Results

In the present study a collection of 101 archived STEC strains isolated from various sources in Iran (2009–2016) were analyzed for the detection of different genes encoding the subtilase variants, plasmidic and chromosomal virulence genes, together with the phylogroup and serogroups. Overall, 57 isolates (56.4%) carried at least one variant of subAB. Most strains from small ruminants including 93% of sheep and 96% of caprine isolates carried at least one chromosomally encoded variant (subAB-2-1 and/or subAb2-2). In contrast, 12 cattle isolates (24%) only harbored the plasmid encoded variant (subAB1). STEC strains from other sources including deer, pony and humans were positive for subAB-2-1 and/or subAb2-2. Concerning the virulence markers, some strains showed an association with hosts the bacteria were isolated from. In particular, tia was associated with sheep, goats and pony isolates and astA gene was present in deer, pony and goats and terD was only found in deer and pony isolates. Only cattle STEC carried espP and epeA, the important markers of pO113 plasmid. Some genes were widespread among strain of various sources like ehly, iha and lpfO113 and some genes were not detected such as efa1, toxB and katP. Most strains belonged to phylogenetic group B1 (89.47%), but five strains from cattle, deer, pony and a goat were assigned to A phylogroup. Most cattle strains belonged to O113, while O5 was just detected in ovine isolates, and O128 and O113 were present in caprine strains.
Conclusions

the present study reveals the presence of potentially pathogenic genotypes among LEE-negative isolates and some host specificity related to subtilase variants and other virulence markers that may aid in source tracking of STEC during outbreak investigations.