bluuebunny/crossref_metadata_2025_split huggingface dataset:
IL-15-induced Lymphocytes as Adjuvant Cellular Immunotherapy for Gastric Cancer
Authors: Hu Yuefeng , Liu Dong , Cui Peilin , Zhang Wen , Chen Hao , Piao Chunmei , Lu Yongcheng , Liu Xuesong , Wang Yue , liu Jingwei , Lu Xu 
Published: July 2021

Abstract
To test the antitumor potential of adoptive cell therapy (ACT)-transferred lymphocytes in a mouse model of human gastric cancer and evaluate the clinical efficacy and safety of combining lymphocytes as adjuvant therapy with first-line chemotherapy in patients with gastric cancer.A human gastric cancer xenograft model was constructed in sublethally irradiated 6–8-week-old NCG male mice. MKN-45 cells (1 × 106 cells/mouse) were subcutaneously injected into the flanks. After tumors had become palpable, the mice were randomized into control, ACTIL-2, and ACTIL-15 groups. Human lymphocytes were injected into the mouse tail veins. In addition, 63 patients who had histological or cytologically confirmed stage III–IV gastric cancer randomly received S-1 plus oxaliplatin plus ACTIL-15 (a combination therapy group) or S-1 plus oxaliplatin alone (chemotherapy group).In the mice, treatment with ACTIL-15 cells inhibited tumor growth upon adoptive transfer, and mice that received ACTIL-15 cells had a significantly longer survival (P<0.05, ACTIL-15 vs. ACTIL-2).In the human study, the median survival of patients in the combination therapy group was 472 days (95% confidence interval (CI), 276–668 days), whereas the median survival of patients in the chemotherapy group was 266 days (95% CI, 200–332 days; P<0.05). Eleven percent (7/63) of patients had adverse reactions, but the reactions did not interfere with treatment.Adoptive transfer of ACTIL-15 cells in a mouse model of gastric cancer and patients with advanced gastric cancer treated with S1 and oxaliplatin improved survival with an acceptable safety profile.
Fatigability is among the factors of discoordination between heart rate variability and physical acceleration during free-moving days in non-older women
Authors: Taniguchi Kentaro , Shimouchi Akito , Jinno Naoya , Seiyama Akitoshi 
Published: July 2021

Abstract
Fatigability is related to several diseases as well as the autonomic nervous system. We investigated whether fatigability is associated with the coordination between the physical acceleration (PA) and heart rate variability (HRV) in women. Overall, 95 adult women were divided into non-old (n=50; age: 22–59 years) and old group (n=45; age: ≥ 60 years). HRV and PA data were simultaneously obtained every minute for 24 h. We defined %lag0 as the percent ratio of lag=0 min between HRV and PA in 1 h. Cornell Medical Index was used to determine the degrees of physical and psychological symptoms. In the non-older group in the hour before sleep, the participants with high fatigability scores had significantly lower %lag0 than those with low fatigability %lag0 (p<0.05). Additionally, those with higher fatigability combined with exhaustion in the morning had significantly lower %lag0 than those without exhaustion in the hour before sleep (p<0.05) but not in the hour after waking up. These results suggest that fatigability in non-older women was associated with the discoordination between HRV and PA in the hour before sleep. Additionally, exhaustion in the morning may be related to the discoordination of HRV and PA the previous night.
Implementation of Evidence-based Guidance for Dementia Palliative Care using Participatory Action Research: Examining Implementation through the Consolidated Framework for Implementation Research (CFIR)
Authors: Coffey Alice , Hartigan Irene , Timmons Suzanne , Buckley Catherine , Lehane Elaine , O'Loughlin Christina , O'Connell Selena , Cornally Nicola 
Published: July 2021

Abstract
Background
The importance of providing evidence-based palliative care for people with dementia is increasingly acknowledged. In Ireland, evidence-based guidance has been developed in order to address key features of dementia palliative care, including the management of pain, medications and hydration and nutrition. The aim of this study was to identify and explore the factors affecting the implementation of evidence-based guidance on dementia palliative care.
Methods
The Consolidated Framework for Implementation (CFIR) guided a mixed-method pre-post study. One guidance document pertaining to the management of pain, medication or hydration and nutrition was implemented in three long-term care facilities. Participatory Action Research in the form of work-based learning groups was used to implement the guidance, drawing on a situational analysis (pre-implementation). Staff questionnaires and audits were conducted pre and post-implementation while champion interviews were also conducted post-implementation.
Results
Features of the guidance (innovation), the inner setting, and the process of implementation were most frequently identified as impacting implementation. Components of the outer setting and individual characteristics featured less commonly. The guidance was perceived as advantageous or complimentary to previous care provided. Within the inner setting, leadership and support from other colleagues facilitated implementation. However, limited availability of other healthcare professionals to assist with carrying out guidance actions presented a barrier in some facilities. The external facilitators of the work-based learning groups (WBLGs) were perceived as experienced and encouraged active participation and reflection on practices. Despite the challenge of releasing staff to attend the WBLGs, a trend toward reduced staff de-motivation among those who did attend was noted post-implementation.
Conclusions
A situational analysis informed by the CFIR framework in conjunction with a participatory action research approach helped to advance implementation of the guidance. The progress of implementation depended on the extent to which evidence-based care was previously being implemented at each site. Post-implementation analysis using CFIR, identified challenges to address in future projects such as staff cover on the ward and timing of training to facilitate attendance for staff with different working hours. Facilitators included multidisciplinary engagement with the intervention and champions at each site to support the implementation process.
Updated Distribution of Anopheline Mosquitoes (Diptera: Culicidae) in Hokkaido, Japan, and the First Evidence of Anopheles (Anopheles) Belenrae in Japan
Authors: Sawabe Kyoko , Imanishi-Kobayashi Nozomi , Maekawa Yoshihide , Higa Yukiko , Kim Kyeong Soon , Hoshino Keita , Tsuda Yoshio , Hayashi Toshihiko , Nihei Naoko , Takai Kenji , Kurihara Takeshi , Kobayashi Mutsuo 
Published: July 2021

Abstract
Background: In Hokkaido, northern island of Japan, at least seven cases of falciparum malaria were reported by 1951. A survey conducted at that time was unsuccessful in implicating any mosquito species as the possible vector. Although active anopheline mosquito surveillance continued until the middle of the 1980s, there is very limited information on their current status and distribution in Japan. Therefore, this study is an update on the current status and distribution of anopheline mosquitoes in Hokkaido based on a 15-year entomological surveillance between 2001 and 2015.Methods: A survey of mosquitoes was conducted at 22 sites in Hokkaido, Japan, from 2001 to 2015. Adult mosquitoes were collected from cowsheds, lakesides, shrubs, and habitats ranging from open grassland to coniferous forest using a CDC miniature light trap enhanced with dry ice, aspirators, and sweeping nets. Larvae were collected from lakes, ponds, swamps, stagnant and flowing rivers, and paddy fields. All specimens were morphologically identified and subjected to PCR-based sequence analysis of the ITS2 region of rDNA. Phylogenetic trees were reconstructed using the neighbor-joining method. Results: A total of 46 anopheline specimens were used for the phylogenetic analysis. During the survey, a new member of the Anopheles hyrcanus group, An. belenrae Rueda (2005), was discovered in eastern Hokkaido in 2004. Anopheles belenrae has since then been consistently found and confirmed to inhabit only this area of Japan. Four members of the An. hyrcanus group, An. belenrae, An. engarensis, An. lesteri, and An. sineroides, have been found in Hokkaido. The results also suggest that An. sinensis, formerly a dominant species throughout Japan, has become a rarely found species, at least currently in Hokkaido.Conclusion: The updated distribution of anopheline mosquitoes in Hokkaido, Japan, showed considerable differences from that observed in previous surveys conducted from 1969 to 1984. In particular, areas where An. sinensis was previously distributed may have been greatly reduced in Hokkaido. The phylogenetic analysis revealed a novel An. hyrcanus group member identified as An. belenrae, described in South Korea in 2005. It is interesting that An. belenrae was confirmed to inhabit only eastern Hokkaido, Japan.
Different Mechanism and Efficacy of Dextran-Sodium Sulfate and 2,4,6-Trinitrobenzene Sulphonic Acid in Modeling Ulcerative Colitis in C57BL/6 Mice
Authors: YE Qiaobo , YE Zhen , WU Mingquan , QIN Kaihua , LU Fating , DENG Qing , WANG Juan , SHE Yingqi , HU Yu , HU Zhipeng , YANG Maoyi , PENG Xi 
Published: July 2021

Abstract
Background and Aims: Dextran-sodium sulfate and 2,4,6-trinitrobenzene sulphonic acid are common modeling methods in studying ulcerative colitis. Little attention has been paid to the mechanism differences between the two approaches. Here, we aim to compare the mechanisms and efficacy of these two models and wish to provide fundamental proves for choosing ideal ulcerative colitis models. Methods: Dextran-sodium sulfate and 2,4,6-trinitrobenzene sulphonic acid were applied to induce the colitis in C57BL/6 mice for seven days. Body weight and disease activity index were assessed. Hematology was detected by routine blood test. Histopathology was analyzed by hematoxylin-eosin staining section. Enzyme-linked immunosorbent assay, Western blot and quantitative real-time PCR were used to detect the cytokines protein levels and mRNA levels. Flow cytometry were used to detect the cycles and subsets of splenic cells. Results: Dextran-sodium sulfate induced colitis in C57BL/6 mice showed higher acute immune activities, while 2,4,6-trinitrobenzene sulphonic acid induced colitis showed chronic immune activities with high platelet amounts and activation. Dextran-sodium sulfate is more suitable for modeling acute ulcerative colitis. On the contrary, 2,4,6-trinitrobenzene sulphonic acid is more appropriate for modeling chronic ulcerative colitis. Conclusions: Dextran-sodium sulfate treatment within 7 days in C57BL/6 mice is a suitable experimental model for studying human acute ulcerative colitis with immune response, fecal blood and acute pathogenic damage. Conversely, 2,4,6-trinitrobenzene sulphonic acid treatment within 7 days is more appropriate for studying human chronic ulcerative colitis with hypercoagulable state, IL-2 over-expression state and chronic pathogenic damage.
Predictive performance and clinical utility of HCC risk scores in chronic hepatitis C: a comparative study
Authors: Shiha Gamal , Mikhail Nabiel NH , Soliman Reham , Hassan Ayman , Eslam Mohammed 
Published: July 2021

Abstract
Background and aim:Many HCC risk prediction scores were developed to guide HCC risk stratification and identify CHC patients who either need intensified surveillance or may not require screening. There is a need to compare different scores and their predictive performance in clinical practice.We aim to compare the newest HCC risk scores evaluating their discriminative ability, and clinical utility in a large cohort of CHC patients.Patients and methodsThe performance of the scores was evaluated in 3075 CHC patients who achieved SVR following DAAs using Log rank, Harrell’s c statistic, also tested for HCC-risk stratification and negative predictive values.ResultsHCC developed in 212 patients within 5 years follow up. Twelve HCC risk scores were identified and displayed significant Log rank (p ≤ 0.05) except Alonso-Lopez TE-HCC, and Chun scores where (p = 0.374, p = 0.053 respectively). Analysis of the remaining ten scores revealed that ADRES, GES pre-post treatment, GES algorithm and Watanabe (post-treatment) scores including dynamics of AFP, were clinically applicable and demonstrated good statistical performance; Log rank analysis < 0.001, Harrell’s C statistic (0.66–0.83) and high negative predictive values (94.38–97.65%). In these three scores, the 5 years Cumulative IR in low risk groups is very low (0.54–1.6) so, screening could be avoid safely in these patients.ConclusionADRES, GES score, GES algorithm and Watanabe (post-treatment) scores seem to offer acceptable HCC-risk predictability and clinical utility in CHC patients. The dynamics of AFP as a component of these scores may explain their high performance when compared to other scores.Word count: 250
Predicting the Future Land Use and Land Cover Changes for Bhavani Basin, Tamil Nadu, India Using QGIS MOLUSCE Plugin
Authors: kamaraj manikandan , Rangarajan Sathyanathan 
Published: July 2021

Abstract
Human population growth, movement, and demand have a substantial impact on land use and land cover dynamics. Thematic maps of land use and land cover (LULC) serve as a reference for scrutinizing, source administration, and forecasting, making it easier to establish plans that balance preservation, competing uses, and growth compressions. The objective of this study is to identify the changeover of land-use changes in the Bhavani basin for the two periods 2005 and 2015, as well as to forecast and establish potential land-use changes in the year 2025 and 2030 by using QGIS 2.18.24 version MOLUSCE plugin (ANN-Multi layer perception) model.The five criteria, such as DEM, gradient, aspect, distance from the road and river, and built-up density, are used as spatial variable maps in the processes of learning in ANN-Multi layer perception to predict their influences on LULC between 2005 and 2010 and it was found that DEM, distance from the road and river, and built-up density have significant effects. The projected and accurate LULC maps for 2015 indicate a good level of accuracy, with an overall Kappa value of 0.69 and with a percentage of the correctness 76.28 %. ANN-Multi-layer perception model is then used to forecast changes in LULC for the years 2025 and 2030 which shows significant rise in cropland and built-up areas, by 20 km2 and 10 km2 respectively. The findings assist farmers and policymakers in developing optimal land use plans and better management techniques for the long-term development of natural resources.
Removal of Ni (II) By Sol-Gel Silica Functionalized With Efficient Metal Chelating Agents
Authors: Naheed Shazia , Syed Shumaila , Amir Tahira , Nawaz Faisal , Bibi Asia , Asghar Faiza , Ahmad Fawad , Ahmad Zaheer , Zakria Ghulam , Zahid Muhammad 
Published: July 2021

Abstract
In this study a comprehensive investigation of doping of sol-gel silica with efficient metal chelating agents is provided at room temperature using infrared spectroscopy, elemental analysis, SEM, EDS and XRD. Sol-gel silica was doped with a series of metal chelating agents to investigate the sorption of Ni (II) ions from aqueous media. It is observed that the chelating agents are encapsulated by the pores of the xerogel from where they interact with the metal ions by ion exchange and chelation mechanism. Nickel complexes of the chelating agents were also synthesized. Sol-gel silica was doped with metal complexes for comparison with sol-gel silica having sorbed metal ions. Same amount of Ni (II) was observed in both samples, showing high efficiency of the method for removal of Ni (II). The batch adsorption experiments were performed at room temperature and neutral pH. The advantage of the method is high sorption capacity at room temperature and neutral pH. The developed method can be applied on large scale removal of toxic heavy metals. The sorption process completed within a minute. 0.1 N HNO3 resulted in complete desorption of metal ions from the gels. The regenerated sorbents were reused several times with negligible loss of sorption capacity.
Regional Forecast of China's Carbon Emission Peak in 2030 and Analysis of Influencing Factors
Authors: Wang Dawei , Shen Wenxing , Zhang Shengliang 
Published: July 2021

Abstract
In this article, we firstly analyzed the historical development trends, regional economies, population, urbanization and carbon emissions of the four major regions which are the East, the Middle, the North-east and the West, respectively. Then we compared China's environmental Kuznets curve both in home and abroad, found that the relationship between economic development and pollution emissions varies from regions and countries. We used the IPAT model to design 48 peak CO2 emission scenarios in order to get to more comprehensive depict of future development path of China's carbon emissions. Furthermore, we used a centralized DEA to evaluate the allocative efficiency of scenario-based CO2 emission paths. As a consequence, we found that maintaining relatively high GDP growth (around 6%), reducing energy intensity by about 75% of average annual decline rate(around 4%); and increasing non-fossil energy to 21% to meet the Innovation plan of energy technology revolution 2016-2030 requirements is the most efficient low-carbon behaviors towards 2030 peak CO2 emissions of 10.57Gt. We also found that China can potentially reduce CO2 emission intensity (CEI) by up to 72.7% compared to the 2005 level in 2030, and therefore that official CEI reduction targets (60-65%) are well within reach. It is also found that in the future path of the predicted optimal scenario, only economic scale and low carbon per unit output value have a great contribution to the changes in carbon dioxide emissions.
Association Between Bone Mineral Density And Serum Creatinine In People &lt; 46 Years Old
Authors: Guo Yanru , Zhu Xianyang 
Published: July 2021

Abstract
Purpose: To research the relationship between serum creatinine and lumbar bone mineral density in people aged <46 years. Methods: A total of 10,968 subjects from the American Nhanes database were included in this cross-sectional study, including 5,744 males (mean age 26.2 years) and 5224 females (mean age 26.7 years). The exposure factor is the serum creatinine value, and the outcome indicator is the lumbar bone mineral density. This study mainly used multivariate linear regression analysis to test the relationship between lumbar bone mineral density and serum creatinine. Results: In the multivariate linear regression analysis, serum creatinine was positively correlated with lumbar bone mineral density (β = 0.122, 95%CI: 0.047-0.198), but in the subgroup analysis stratified by sex, this positive correlation only exists in the female population (Β = 0.186, 95%CI: 0.070-0.301).Conclusions: Our study found that in women aged <46 years with normal renal function, there is a positive correlation between serum creatinine and lumbar BMD. And in those people, the determination of serum creatinine can provide a sensitive biomarker for the early identification and treatment of Osteopenia or osteoporosis.
The Computational Analyses, Molecular Dynamics of Fatty-Acid Transport Mechanism to the CD36 Receptor: The Outcomes of the Mutation K164A on the Binding Domain, Structure and Function
Authors: Akachar Jihane , Etchebest Catherine , Eljaoudi Rachid , Ibrahimi Azeddine 
Published: July 2021

Abstract
The transmembrane glycoprotein CD36, which is responsible of the metabolic disorders, and the elevated intake of fat induces lipid buildup, is a multifunctional scavenger receptor signaling those functions in high-affinity tissue uptake of long-chain fatty acids. In this study, we used series of molecular dynamics simulations of the wild type and mutants types K164A CD36 protein interacting with one Palmitic acid (PLM) besides simulations of the wild type interacting with the three PLM to find out the mechanism of the functioning of the complex CD36/Fatty acids and the unraveling of the role of the mutation. Additionally we determined whether Lys164, mostly exposed to protein surface, played important roles in fatty acid uptake. These simulations revealed, the conformational changes induced by Lys164 residue and the altered interactions induced by the mutagenesis of surface lysine that was badly influencing the folding, utility, solubility, and stability form of the variant. Furthermore, Lys164 residue provided the structural basis of forming an opening at the region of principal portal for the dissociation of palmitic acid. The results of our simulations revealed hole two fatty acids found in CD36 cavity structure and it was the most preferred to CD36 structure stabilization.
Design and Discovery of a High Affinity, Selective and β-Arrestin Biased 5-HT7 Receptor Agonist
Authors: Onyameh Edem K. , Ofori Edward , Bricker Barbara A. , Gonela Uma M. , Eyunni Suresh V. K. , Kang Hye J. , Voshavar Chandrashekar , Ablordeppey Seth Y 
Published: July 2021

Abstract
Compound 1c, 2-(2-(3,4-Dihydroisoquinolin-2(1H)-yl)ethyl)-5-fluoro-2,3-dihydro-1H-inden-1-one was previously reported from our laboratory showing high affinity binding to the 5-HT7 receptor (Ki = 0.5 nM). However, compound 1c racemizes readily upon enantiomeric separation. To prevent racemization, we have redesigned and synthesized a methyl and carboxyethyl analogs, compounds 2 and 3 respectively, whose binding affinities were similar to those of compound 1c. Compound 2 and 3 cannot undergo racemization since tautomerism was no longer possible and thus, compound 2 was selected for enantiomeric separation and further evaluation. Upon enantiomeric separation, the levorotatory enantiomer, (-)2 or 2a demonstrated a higher affinity and a β-arrestin biased functional selectivity for the 5-HT7 receptor. Although 2a showed 22 times less activity than 5-HT in the Gs pathway, it showed 28 times higher activity than 5-HT in the β-arrestin pathway. This constitutes a significant β-arrestin pathway preference and shows 2a to be more potent and more efficacious than the recently published β-arrestin biased 3-(4-chlorophenyl)-1,4,5,6,7,8-hexahydropyrazolo[3,4-d]azepine, the N-debenzylated analog of JNJ18038683 (Compound 7).
Bioconversion of Apple Pomace into Microbial Protein Feed Based on Extrusion Pretreatment
Authors: Yang Zhe , Jiang Lijun , Zhang Min , Deng Yuxin , Suo Wenjing , Zhang Haijing , Wang Chenjie , Li Hongjun 
Published: July 2021

Abstract
Apple pomace (AP) is often used directly as animal feed, while the value of feeding is limited by its low protein content. In this study, extrusion pretreatment was performed for AP, and further fermentation was carried out to improve its nutrition value. Strains suitable for extruded apple pomace (EAP) to produce high-quality microbial protein (MP) feed were screened from 12 different strains. Results showed that Aspergillus niger 3.324 (Asn), Candida utilis1314 (Cau), Geotrichum candidum 1315 (Gec), Bacillus subtilis A308 (Bas1) and Lactic acid bacteria (Lac) were screened as the dominant strains, which exhibited higher feeding value. Strongsymbiotic effect was observed in fermentation with mixed strains of Asn, Cau, Gec, and Lacat the ratio of 1:1:1:1. Compared with AP, the pure protein content in the optimized fermented EAP (FEAP) was increased by 138% accompanying with a pleasant flavor and taste. The nutrition value of FEAP was characterized by amino acid profiles, it found that FEAP was comparable to other commercial proteins with higher contents of histidine, phenylalanine, threonine and valine. Combination of fermentation and extrusion technology significantly enhanced pure protein content and nutritional composition of apple pomace, which was revalorized as a nutritive animal feed that rich in microbial protein.