bluuebunny/crossref_metadata_2025_split huggingface dataset:
Crime Victims, Immigrants And Social Welfare: Creating The Racialized Other In Sweden
Authors: Branteryd Fia , Gallo Carina , Brown Elizabeth , Svensson Kerstin 
Published: August 2021

AbstractThis study explores the role of immigration and racialization in creating the first Swedish victim support centre in the early 1980s. The study is based on a qualitative content analysis of the archives of Victim Support Södertälje, the first lasting Swedish victim support centre, from 1983 to 1990. While many of the centre’s activities focused on crime prevention, it defined crime and victimization as the province of immigrant communities in Södertälje, and, notably, as outside—and aberrant from—the imagined racial community of Sweden. Thus, victim support centres were one of the mechanisms to continue to defend the ideals of the Swedish welfare state, but to do so in ways that prevented incursions within Swedish racial homogeneity from the outside.
Short-term outcomes of a multicentre randomized clinical trial comparing laparoscopic pylorus-preserving gastrectomy with laparoscopic distal gastrectomy for gastric cancer (the KLASS-04 trial)
Authors: Park D. J. , Kim Y.-W. , Yang H.-K. , Ryu K. W. , Han S.-U. , Kim H.-H. , Hyung W.-J. , Park J. H. , Suh Y.-S. , Kwon O. K. , Yoon H. M. , Kim W. , Park Y.-K. , Kong S.-H , Ahn S. H. , Lee H.-J. 
Published: August 2021

Abstract

Background
There remain concerns about the safety and functional benefit of laparoscopic pylorus-preserving gastrectomy (LPPG) compared with laparoscopic distal gastrectomy (LDG). This study evaluated short-term outcomes of a randomized clinical trial (RCT) comparing LPPG with LDG for gastric cancer.

Methods
The Korean Laparoendoscopic Gastrointestinal Surgery Study (KLASS)-04 trial was an investigator-initiated, open-label, parallel-assigned, superiority, multicentre RCT in Korea. Patients with cT1N0M0 cancer located in the middle third of the stomach at least 5 cm from the pylorus were randomized to undergo LPPG or LDG. Participants, care givers and those assessing the outcomes were not blinded to group assignment. Outcomes were 30-day postoperative morbidity rate and death at 90 days.

Results
Some 256 patients from nine institutions were randomized (LPPG 129 patients, LDG 127 patients) between July 2015 and July 2017 and outcomes for 253 patients were analysed. Postoperative complications within 30 days were seen in 19.3 and 15.5 per cent in the LPPG and LDG groups respectively (P = 0·419). Postoperative pyloric stenosis was observed in nine (7.2 per cent) and two (1·5 per cent) patients in the LPPG and LDG groups (P = 0·026) respectively. In multivariable analysis higher BMI was a risk factor for postoperative complications (odds ratio 1·17, 95 per cent c.i. 1·04 to 1·32; P = 0·011). Death at 90 days was zero in both groups.

Conclusion
Postoperative complications and mortality was comparable in patients undergoing LPPG and LDG. Registration number: NCT02595086 (http://www.clinicaltrials.gov).
Interactome screening ofC9orf72dipeptide repeats reveals VCP sequestration and functional impairment by polyGA
Authors: Božič Janja , Motaln Helena , Janež Anja Pucer , Markič Lara , Tripathi Priyanka , Yamoah Alfred , Aronica Eleonora , Lee Youn-Bok , Heilig Raphael , Fischer Roman , Thompson Andrew J , Goswami Anand , Rogelj Boris 
Published: August 2021

AbstractRepeat expansions in the C9orf72 gene are a common cause of amyotrophic lateral sclerosis and frontotemporal lobar degeneration, two devastating neurodegenerative disorders. One of the proposed mechanisms of GGGGCC repeat expansion is their translation into non-canonical dipeptide repeats, which can then accumulate as aggregates and contribute to these pathologies. There are five different dipeptide repeat proteins (polyGA, polyGR, polyPR, polyPA and polyGP), some of which are known to be neurotoxic.In the present study, we used BioID2 proximity labelling to identify the interactomes of all five dipeptide repeat proteins consisting of 125 repeats each. We identified 113 interacting partners for polyGR, 90 for polyGA, 106 for polyPR, 25 for polyPA and 27 for polyGP. Gene Ontology enrichment analysis of the proteomic data revealed that these target interaction partners are involved in a variety of functions, including protein translation, signal transduction pathways, protein catabolic processes, amide metabolic processes and RNA-binding. Using autopsy brain tissue from patients with C9orf72 expansion complemented with cell culture analysis, we evaluated the interactions between polyGA and valosin containing protein (VCP). Functional analysis of this interaction revealed sequestration of VCP with polyGA aggregates, altering levels of soluble valosin-containing protein. VCP also functions in autophagy processes, and consistent with this, we observed altered autophagy in cells expressing polyGA. We also observed altered co-localization of polyGA aggregates and p62 in cells depleted of VCP.All together, these data suggest that sequestration of VCP with polyGA aggregates contributes to the loss of VCP function, and consequently to alterations in autophagy processes in C9orf72 expansion disorders.
Functional Inhibitory Connections Modulate the Electrophysiological Activity Patterns of Cortical-Hippocampal Ensembles
Authors: Brofiga Martina , Pisano Marietta , Tedesco Mariateresa , Boccaccio Anna , Massobrio Paolo 
Published: August 2021

Abstract
The brain is a complex organ composed of billions of neurons connected through excitatory and inhibitory synapses. Its structure reveals a modular topological organization, where neurons are arranged in interconnected assemblies. The generated patterns of electrophysiological activity are shaped by two main factors: network heterogeneity and the topological properties of the underlying connectivity that strongly push the dynamics toward different brain-states. In this work, we exploited an innovative polymeric structure coupled to Micro-Electrode Arrays (MEAs) to recreate in vitro heterogeneous interconnected (modular) neuronal networks made up of cortical and hippocampal neurons. We investigated the propagation of spike sequences between the two interconnected subpopulations during the networks’ development, correlating functional and structural connectivity to dynamics. The simultaneous presence of two neuronal types shaped the features of the functional connections (excitation vs. inhibition), orchestrating the emerging patterns of electrophysiological activity. In particular, we found that hippocampal neurons mostly project inhibitory connections toward the cortical counterpart modulating the temporal scale of the population events (network bursts). In contrast, cortical neurons establish a larger amount of intrapopulation connections. Moreover, we proved topological properties such as small-worldness, degree distribution, and modularity of neuronal assemblies were favored by the physical environment where networks developed and matured.
Determination of Saccharides in Honey Using Supercritical Fluid Chromatography Coupled with Single Quadrupole Mass Spectrometry
Authors: Huang Yang , Jiang Zhengjin 
Published: August 2021

Abstract
Saccharides are one of the most important biomoleculars in the world, which are responsible for various roles in living organisms. In this work, a combined use of mass spectrometry (MS) together with supercritical fluid chromatography (SFC) was applied for the separation of eight saccharides. A satisfactory separation was achieved within 15 min on a Unitary Diol column using 10 mM ammonium acetate in methanol as mobile phase additive at a temperature of 60°C. Compared to liquid chromatography–MS (LC–MS), the SFC–MS presented faster running time and better selectivity. The SFC–MS was validated and applied to the analysis of three saccharides (fructose, glucose and sucrose) present in different honey samples. The limits of detection (LODs) and limits of quantification (LOQs) of SFC–MS method for fructose, glucose and sucrose were 5.2, 4.5, 7.4 μg/mL and 17.2, 14.9, 24.1 μg/mL, respectively. The relative standard deviations for intrabatch and interbatch precision were &lt;2.5% and the recoveries ranged from 97.6% to 103.1% for three concentration levels. The values of LODs and LOQs using SFC–MS method were lower (1.5–2.4-fold) than using LC–MS method. The results demonstrated the potential of SFC–MS for fast and sensitive determination of saccharides in honey.
Do you feel itchy? A guide towards diagnosis and measurement of chronic kidney disease-associated pruritus in dialysis patients
Authors: Manenti Lucio , Leuci Emanuela 
Published: August 2021

ABSTRACT
Chronic kidney disease-associated pruritus (CKD-aP) is a potentially distressing condition that affects a significant proportion of patients with end-stage kidney disease undergoing dialysis. CKD-aP may lead to worsening of patients’ physical and mental health-related quality of life (HRQoL) and has also been linked with worse clinical outcomes, including increased mortality. Despite these detrimental effects, evidence from real-world studies shows that CKD-aP still remains overlooked by nephrologists and underreported by patients in clinical practice. Itch is subjective and therefore its diagnosis is often dependent on patients reporting this symptom. There is an opportunity to reduce the burden of CKD-aP on dialysis patients by increasing awareness about this condition and the availability of effective treatments. It is particularly important that nephrologists and other healthcare providers routinely ask their patients if they are experiencing itch. The differential diagnosis of CKD-aP requires a step-by-step identification and exclusion of possible alternative or concomitant causes of itch. Several simple validated self-reported assessment scales are available to evaluate the presence and severity of itch in a time-efficient manner, making them suitable for use in everyday clinical practice. The impact of CKD-aP on haemodialysis patients’ HRQoL should also be assessed on a regular basis. This review provides a comprehensive overview of the differential diagnosis of CKD-aP and the diagnostic tools that are available to identify itch and quantify its severity and impact on patient HRQoL. A suggested algorithm to guide the screening, diagnosis and assessment of CKD-aP among dialysis patients in real-world practice is provided.
Novel biomarker-driven prognostic models to predict morbidity and mortality in chronic heart failure: the EMPEROR-Reduced trial
Authors: Pocock Stuart J , Ferreira João Pedro , Gregson John , Anker Stefan D , Butler Javed , Filippatos Gerasimos , Gollop Nicholas D , Iwata Tomoko , Brueckmann Martina , Januzzi James L , Voors Adriaan A , Zannad Faiez , Packer Milton 
Published: August 2021

Abstract

Aims
The aim of this study was to generate a biomarker-driven prognostic tool for patients with chronic HFrEF. Circulating levels of N-terminal pro B-type natriuretic peptide (NT-proBNP) and high-sensitivity cardiac troponin T (hs-cTnT) each have a marked positive relationship with adverse outcomes in heart failure with reduced ejection fraction (HFrEF). A risk model incorporating biomarkers and clinical variables has not been validated in contemporary heart failure (HF) trials.

Methods and results
In EMPEROR-Reduced, 33 candidate variables were pre-selected. Multivariable Cox regression models were developed using stepwise selection for: (i) the primary composite outcome of HF hospitalization or cardiovascular death, (ii) all-cause death, and (iii) cardiovascular mortality. A total of 3730 patients were followed up for a median of 16 months, 823 (22%) patients had a primary outcome and 515 (14%) patients died, of whom 389 (10%) died from a cardiovascular cause. NT-proBNP and hs-cTnT were the dominant predictors of the primary outcome, and in addition, a shorter time since last HF hospitalization, longer time since HF diagnosis, lower systolic blood pressure, New York Heart Association (NYHA) Class III or IV, higher heart rate and peripheral oedema were key predictors (eight variables in total, all P &lt; 0.001). The primary outcome risk score discriminated well (c-statistic = 0.73), with patients in the top 10th of risk having an event rate &gt;9 times higher than those in the bottom 10th. Empagliflozin benefitted patients across risk levels for the primary outcome. NT-proBNP and hs-cTnT were also the dominant predictors of all-cause and cardiovascular mortality, followed by NYHA Class III or IV and ischaemic aetiology (four variables in total, all P &lt; 0.001). The mortality risk model presented good event discrimination for all-cause and cardiovascular mortality (c-statistic = 0.69 for both). These simple models were externally validated in the BIOSTAT-CHF study, achieving similar c-statistics.

Conclusions
The combination of NT-proBNP and hs-cTnT with a small number of readily available clinical variables provides prognostic assessment for patients with HFrEF. This predictive tool kit can be easily implemented for routine clinical use.
The use of remote monitoring of cardiac implantable devices during the COVID-19 pandemic: an EHRA physician survey
Authors: Simovic Stefan , Providencia Rui , Barra Sergio , Kircanski Bratislav , Guerra Jose M , Conte Giulio , Duncker David , Marijon Eloi , Anic Ante , Boveda Serge 
Published: August 2021

Abstract
It is unclear to what extent the COVID-19 pandemic has influenced the use of remote monitoring (RM) of cardiac implantable electronic devices (CIEDs). The present physician-based European Heart Rhythm Association (EHRA) survey aimed to assess the influence of the COVID-19 pandemic on RM of CIEDs among EHRA members and how it changed the current practice. The survey comprised 27 questions focusing on RM use before and during the pandemic. Questions focused on the impact of COVID-19 on the frequency of in-office visits, data filtering, reasons for initiating in-person visits, underutilization of RM during COVID-19, and RM reimbursement. A total of 160 participants from 28 countries completed the survey. Compared to the pre-pandemic period, there was a significant increase in the use of RM in patients with pacemakers (PMs) and implantable loop recorders (ILRs) during the COVID-19 pandemic (PM 24.2 vs. 39.9%, P = 0.002; ILRs 61.5 vs. 73.5%, P = 0.028), while there was a trend towards higher utilization of RM for cardiac resynchronization therapy-pacemaker (CRT-P) devices during the pandemic (44.5 vs. 55%, P = 0.063). The use of RM with implantable cardioverter-defibrillators (ICDs) and CRT-defibrillator (CRT-D) did not significantly change during the pandemic (ICD 65.2 vs. 69.6%, P = 0.408; CRT-D 65.2 vs. 68.8%, P = 0.513). The frequency of in-office visits was significantly lower during the pandemic (P &lt; 0.001). Nearly two-thirds of participants (57 out of 87 respondents), established new RM connections for CIEDs implanted before the pandemic with 33.3% (n = 29) delivering RM transmitters to the patient’s home address, and the remaining 32.1% (n = 28) activating RM connections during an in-office visit. The results of this survey suggest that the crisis caused by COVID-19 has led to a significant increase in the use of RM of CIEDs.
Docosahexaenoic acid normalizes QT interval in long QT type 2 transgenic rabbit models in a genotype-specific fashion
Authors: Castiglione Alessandro , Hornyik Tibor , Wülfers Eike M , Giammarino Lucilla , Edler Iask , Jowais Jessica J , Rieder Marina , Perez-Feliz Stefanie , Koren Gideon , Bősze Zsuzsanna , Varró András , Zehender Manfred , Brunner Michael , Bode Christoph , Liin Sara I , Larsson Hans Peter , Baczkó István , Odening Katja E 
Published: August 2021

Abstract

Aim
Long QT syndrome (LQTS) is a cardiac channelopathy predisposing to ventricular arrhythmias and sudden cardiac death. Since current therapies often fail to prevent arrhythmic events in certain LQTS subtypes, new therapeutic strategies are needed. Docosahexaenoic acid (DHA) is a polyunsaturated fatty acid, which enhances the repolarizing IKs current.

Methods and results
We investigated the effects of DHA in wild type (WT) and transgenic long QT Type 1 (LQT1; loss of IKs), LQT2 (loss of IKr), LQT5 (reduction of IKs), and LQT2–5 (loss of IKr and reduction of IKs) rabbits. In vivo ECGs were recorded at baseline and after 10 µM/kg DHA to assess changes in heart-rate corrected QT (QTc) and short-term variability of QT (STVQT). Ex vivo monophasic action potentials were recorded in Langendorff-perfused rabbit hearts, and action potential duration (APD75) and triangulation were assessed. Docosahexaenoic acid significantly shortened QTc in vivo only in WT and LQT2 rabbits, in which both α- and β-subunits of IKs-conducting channels are functionally intact. In LQT2, this led to a normalization of QTc and of its short-term variability. Docosahexaenoic acid had no effect on QTc in LQT1, LQT5, and LQT2–5. Similarly, ex vivo, DHA shortened APD75 in WT and normalized it in LQT2, and additionally decreased AP triangulation in LQT2.

Conclusions
Docosahexaenoic acid exerts a genotype-specific beneficial shortening/normalizing effect on QTc and APD75 and reduces pro-arrhythmia markers STVQT and AP triangulation through activation of IKs in LQT2 rabbits but has no effects if either α- or β-subunits to IKs are functionally impaired. Docosahexaenoic acid could represent a new genotype-specific therapy in LQT2.
Three-dimensional inversion for sparse potential data using first-order system least squares with application to gravity anomalies in Western Queensland
Authors: Codd A L , Gross L 
Published: August 2021

SUMMARY
We present an inversion algorithm tailored for point gravity data. As the data are from multiple surveys, it is inconsistent with regards to spacing and accuracy. An algorithm design objective is the exact placement of gravity observations to ensure no interpolation of the data is needed prior to any inversion. This is accommodated by discretization using an unstructured tetrahedral finite-element mesh for both gravity and density with mesh nodes located at all observation points and a first-order system least-squares (FOSLS) formulation for the gravity modelling equations. Regularization follows the Bayesian framework where we use a differential operator approximation of an exponential covariance kernel, avoiding the usual requirement of inverting large dense covariance matrices. Rather than using higher order basis functions with continuous derivatives across element faces, regularization is also implemented with a FOSLS formulation using vector-valued property function (density and its gradient). Minimization of the cost function, comprised of data misfit and regularization, is achieved via a Lagrange multiplier method with the minimum of the gravity FOSLS functional as a constraint. The Lagrange variations are combined into a single equation for the property function and solved using an integral form of the pre-conditioned conjugate gradient method (I-PCG). The diagonal entries of the regularization operator are used as the pre-conditioner to minimize computational costs and memory requirements. Discretization of the differential operators with the finite-element method (FEM) results in matrix systems that are solved with smoothed aggregation algebraic multigrid pre-conditioned conjugate gradient (AMG-PCG). After their initial setup, the AMG-PCG operators and coarse grid solvers are reused in each iteration step, further reducing computation time. The algorithm is tested on data from 23 surveys with a total of 6519 observation points in the Mt Isa–Cloncurry region in north–west Queensland, Australia. The mesh had about 2.5 million vertices and 16.5 million cells. A synthetic case was also tested using the same mesh and error measures for localized concentrations of high and low densities. The inversion results for different parameters are compared to each other as well as to lower order smoothing. Final inversion results are shown with and without depth weighting and compared to previous geological studies for the Mt Isa–Cloncurry region.
Dissecting the transcriptional program of phosphomannomutase 2-deficient cells: Lymphoblastoide B cell lines as a valuable model for congenital disorders of glycosylation studies
Authors: Parrado Antonio , Rubio Gonzalo , Serrano Mercedes , De la Morena-Barrio María Eugenia , Ibáñez-Micó Salvador , Ruiz-Lafuente Natalia , Schwartz-Albiez Reinhard , Esteve-Solé Ana , Alsina Laia , Corral Javier , Hernández-Caselles Trinidad 
Published: August 2021

AbstractCongenital disorders of glycosylation (CDG) include 150 genetically and clinically heterogeneous diseases, showing significant glycoprotein hypoglycosylation that leads to pathological consequences in multiple organs and systems whose underlying mechanisms are not yet understood. A few cellular and animal models have been used to study specific CDG characteristics, although they have given limited information due to the few CDG mutations tested and the still missing comprehensive molecular and cellular basic research. Here, we provide specific gene expression profiles, based on ribonucleic acid (RNA) microarray analysis, together with some biochemical and cellular characteristics of a total of nine control Epstein–Barr virus-transformed lymphoblastoid B cell lines (B-LCL) and 13 CDG B-LCL from patients carrying severe mutations in the phosphomannomutase 2 (PMM2) gene, strong serum protein hypoglycosylation and neurological symptoms. Significantly dysregulated genes in PMM2-CDG cells included those regulating stress responses, transcription factors, glycosylation, motility, cell junction and, importantly, those related to development and neuronal differentiation and synapse, such as carbonic anhydrase 2 (CA2) and ADAM23. PMM2-CDG-associated biological consequences involved the unfolded protein response, RNA metabolism and the endoplasmic reticulum, Golgi apparatus and mitochondria components. Changes in the transcriptional and CA2 protein levels are consistent with the CDG physiopathology. These results demonstrate the global transcriptional impact in phosphomannomutase 2-deficient cells, reveal CA2 as a potential cellular biomarker and confirm B-LCL as an advantageous model for CDG studies.