bluuebunny/crossref_metadata_2025_split huggingface dataset:
Synthesis, characterization, and pharmacological evaluation of some metal complexes of quercetin as P-gp inhibitors
Authors: Shastrala Kirankumar , Kalam Sirisha , Damerakonda Kumaraswamy , Sheshagiri Sharvana Bhava Bandaru , Kumar Hitesh , Guda Ramu , Kasula Mamatha , Bedada Satish Kumar 
Published: May 2021

AbstractBackgroundSix different metal complexes of quercetin (Cu, Zn, Co, Vd, Mo, Ni) were synthesized, purified, and characterized by their physical and spectral (UV, IR) data. They were evaluated for their P-gp (permeability glycoprotein) inhibitory activity by in vitro everted sac method in rats. The apparent permeability of atorvastatin (P-gp substrate) from everted sac of the rat intestine was determined in control, standard (verapamil), and groups treated with quercetin-metal complexes. The drug contents were analyzed by validated RP-HPLC method using a mixture of acetonitrile and water (60:40 v/v) adjusted to pH 2.8 with phosphate buffer as mobile phase.ResultsIn vitro studies revealed that the apparent permeability of atorvastatin (P-gp substrate) across the small intestine is much affected by the treatment with Cu/Co/Ni complexes of quercetin. The mean ± SD and apparent permeability of atorvastatin decreased after pre-treatment with these metal complexes.ConclusionsThe quercetin Cu/Co/Ni complexes could inhibit P-gp and increase the atorvastatin absorption. Hence, they could be considered P-gp inhibitors.
Bacterial isolates and their antibiotic sensitivity pattern of surgical site infections among the surgical ward patients of Asella Referral and Teaching Hospital
Authors: Gebissa Teshome , Bude Bonge , Yasir Mohd , Mekit Shimelis , Noorulla K. M. 
Published: May 2021

Abstract
Background
Surgical site infection (SSI) is the most typical problem for patients who undergo operative procedures. It remains a typical and widespread problem causing morbidity and mortality, partly related to a rise in infections due to antimicrobial-resistant bacterial pathogens. The study was purposed to evaluate the bacterial isolates and their drug susceptibility patterns in patients with postoperative surgical site infection.

Results
The rate of postoperative surgical site infections was studied at Asella Referral and Teaching Hospital and found to be 23.3%. One hundred fifty specimens of pus and surface swabs were collected from the surgical site-infected patients over the period of March 2016 to May 2017, and from that, a total of 147 bacterial pathogens were recovered. The predominant organisms associated with postoperative surgical site infections were Klebsiella species 38 (26%, n=147), Escherichia coli 31 (21%, n=147), Staphylococcus aureus 25 (17%, n=147), and Pseudomonas aeruginosa 18 (12%, n=147).

Conclusion
Higher number of bacterial isolates were recovered. The predominant isolates were Klebsiella species, Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa. The majority of gram-negative bacterial species which were isolated were found to be resistant to the commonly prescribed antimicrobial agents in the study setting. Thus, to achieve effective therapy for wound infections and to reduce/stop the appearance of multidrug-resistant (MDR) pathogens, continuous monitoring is essential with the fair use of antimicrobial agents.
DXA-Based Bone Strain Index: A New Tool to Evaluate Bone Quality in Primary Hyperparathyroidism
Authors: Tabacco Gaia , Naciu Anda M , Messina Carmelo , Sanson Gianfranco , Rinaudo Luca , Cesareo Roberto , Falcone Stefania , Manfrini Silvia , Napoli Nicola , Bilezikian John P , Ulivieri Fabio M , Palermo Andrea 
Published: May 2021

Abstract

Context
Primary hyperparathyroidism (PHPT) is associated with impaired bone quality and increased fracture risk. Reliable tools for the evaluation of bone quality parameters are not yet clinically available. Bone Strain Index (BSI) is a new metric for bone strength based on Finite Element Analysis from lumbar spine and femoral neck dual-energy x-ray absorptiometry (DXA) images.

Objective
To assess the lumbar spine (LS), femoral neck (FN), and total hip (TH) BSI in PHPT patients compared with controls and to investigate the association of BSI with vertebral fractures (VFs) in PHPT.

Methods
This case-control study enrolled 50 PHPT patients and 100 age- and sex-matched control subjects from an outpatient clinic. The main outcome measures were LS-BSI, FN-BSI, and TH-BSI.

Results
FN bone mineral density (BMD) and one-third distal radius BMD were lower in the PHPT group than in controls (FN 0.633 ± 0.112 vs 0.666 ± 0.081, P = 0.042; radius 0.566 ± 0.07 vs 0.625 ± 0.06, P &lt; 0.001). PHPT group has significant lower TBS score compared with controls (1.24 ± 0.09 vs 1.30 ± 0.10, P &lt; 0.001). BSI was significantly higher at LS (2.28 ± 0.59 vs 2.02 ± 0.43, P = 0.009), FN (1.72 ± 0.41 vs 1.49 ± 0.35, P = 0.001), and TH (1.51 ± 0.33 vs 1.36 ± 0.25, P = 0.002) in PHPT. LS-BSI showed moderate accuracy for discriminating VFs (AUC 0.667; 95% CI, 0.513-0.820). LS-BSI ≥ 2.2 and was a statistically significant independent predictor of VFs, with an adjusted odds ratio ranging from 5.7 to 15.1.

Conclusion
BSI, a DXA-derived bone quality index, is impaired in PHPT and may help to identify PHPT subjects at high risk of fractures.
A Novel Mouse Model for SNP in Steroid Receptor Co-Activator-1 Reveals Role in Bone Density and Breast Cancer Metastasis
Authors: Watters Rebecca J , Verdelis Kostas , Lucas Peter C , Jiang Shiming , Chen Yuqing , Lu Feiqi , Martin Benjamin M , Lukashova Lyuda , Pecar Geoffrey , Morales-Restrepo Alejandro , Hankins Margaret , Zhu Li , Mittwede Peter , Hartmaier Ryan J , Alexander Peter G , Tseng George C , Weiss Kurt R , Galson Deborah L , Lee Adrian V , Lee Brendan , Oesterreich Steffi 
Published: May 2021

Abstract
The steroid receptor coactivator-1 (SRC-1) is a nuclear receptor co-activator, known to play key roles in both estrogen response in bone and in breast cancer metastases. We previously demonstrated that the P1272S single nucleotide polymorphism (SNP; P1272S; rs1804645) in SRC-1 decreases the activity of estrogen receptor in the presence of selective estrogen receptor modulators (SERMs) and that it is associated with a decrease in bone mineral density (BMD) after tamoxifen therapy, suggesting it may disrupt the agonist action of tamoxifen. Given such dual roles of SRC-1 in the bone microenvironment and in tumor cell-intrinsic phenotypes, we hypothesized that SRC-1 and a naturally occurring genetic variant, P1272S, may promote breast cancer bone metastases. We developed a syngeneic, knock-in mouse model to study if the SRC-1 SNP is critical for normal bone homeostasis and bone metastasis. Our data surprisingly reveal that the homozygous SRC-1 SNP knock-in increases tamoxifen-induced bone protection after ovariectomy. The presence of the SRC-1 SNP in mammary glands resulted in decreased expression levels of SRC-1 and reduced tumor burden after orthotopic injection of breast cancer cells not bearing the SRC-1 SNP, but increased metastases to the lungs in our syngeneic mouse model. Interestingly, the P1272S SNP identified in a small, exploratory cohort of bone metastases from breast cancer patients was significantly associated with earlier development of bone metastasis. This study demonstrates the importance of the P1272S SNP in both the effect of SERMs on BMD and the development of tumor in the bone.
The Cholesterol Metabolite 27HC Increases Secretion of Extracellular Vesicles Which Promote Breast Cancer Progression
Authors: Baek Amy E , Krawczynska Natalia , Das Gupta Anasuya , Dvoretskiy Svyatoslav Victorovich , You Sixian , Park Jaena , Deng Yu-Heng , Sorrells Janet E , Smith Brandi Patrice , Ma Liqian , Nelson Adam T , McDowell Hannah B , Sprenger Ashabari , Henn Madeline A , Madak-Erdogan Zeynep , Kong Hyunjoon , Boppart Stephen A , Boppart Marni D , Nelson Erik R 
Published: May 2021

AbstractCholesterol has been implicated in the clinical progression of breast cancer, a disease that continues to be the most commonly diagnosed cancer in women. Previous work has identified the cholesterol metabolite 27-hydroxycholesterol (27HC) as a major mediator of the effects of cholesterol on breast tumor growth and progression. 27HC can act as an estrogen receptor (ER) modulator to promote the growth of ERα+ tumors, and as a liver X receptor (LXR) ligand in myeloid immune cells to establish an immune-suppressive program. In fact, the metastatic properties of 27HC require the presence of myeloid cells with neutrophils (polymorphonuclear neutrophils; PMNs) being essential for the increase in lung metastasis in murine models. In an effort to further elucidate the mechanisms by which 27HC alters breast cancer progression, we made the striking finding that 27HC promoted the secretion of extracellular vesicles (EVs), a diverse assortment of membrane bound particles that includes exosomes. The resulting EVs had a size distribution that was skewed slightly larger than EVs generated by treating cells with vehicle. The increase in EV secretion and size was consistent across 3 different subtypes: primary murine PMNs, RAW264.7 monocytic cells, and 4T1 murine mammary cancer cells. Label-free analysis of 27HC-EVs indicated that they had a different metabolite composition to those from vehicle-treated cells. Importantly, 27HC-EVs from primary PMNs promoted tumor growth and metastasis in 2 different syngeneic models, demonstrating the potential role of 27HC-induced EVs in the progression of breast cancer. EVs from PMNs were taken up by cancer cells, macrophages, and PMNs, but not T cells. Since EVs did not alter proliferation of cancer cells, it is likely that their protumor effects are mediated through interactions with myeloid cells. Interestingly, RNA-seq analysis of tumors from 27HC-EV-treated mice do not display significantly altered transcriptomes, suggesting that the effects of 27HC-EVs occur early on in tumor establishment and growth. Future work will be required to elucidate the mechanisms by which 27HC increases EV secretion, and how these EVs promote breast cancer progression. Collectively, however, our data indicate that EV secretion and content can be regulated by a cholesterol metabolite, which may have detrimental effects in terms of disease progression, important findings given the prevalence of both breast cancer and hypercholesterolemia.
An RNAi Screening of Clinically Relevant Transcription Factors Regulating Human Adipogenesis and Adipocyte Metabolism
Authors: Björk Christel , Subramanian Narmadha , Liu Jianping , Acosta Juan Ramon , Tavira Beatriz , Eriksson Anders B , Arner Peter , Laurencikiene Jurga 
Published: May 2021

Abstract

Context
Healthy hyperplasic (many but smaller fat cells) white adipose tissue (WAT) expansion is mediated by recruitment, proliferation and/or differentiation of new fat cells. This process (adipogenesis) is controlled by transcriptional programs that have been mostly identified in rodents.

Objective
A systemic investigation of adipogenic human transcription factors (TFs) that are relevant for metabolic conditions has not been revealed previously.

Methods
TFs regulated in WAT by obesity, adipose morphology, cancer cachexia, and insulin resistance were selected from microarrays. Their role in differentiation of human adipose tissue-derived stem cells (hASC) was investigated by RNA interference (RNAi) screen. Lipid accumulation, cell number, and lipolysis were measured for all screened factors (148 TFs). RNA (RNAseq), protein (Western blot) expression, insulin, and catecholamine responsiveness were examined in hASC following siRNA treatment of selected target TFs.

Results
Analysis of TFs regulated by metabolic conditions in human WAT revealed that many of them belong to adipogenesis-regulating pathways. The RNAi screen identified 39 genes that affected fat cell differentiation in vitro, where 11 genes were novel. Of the latter JARID2 stood out as being necessary for formation of healthy fat cell metabolic phenotype by regulating expression of multiple fat cell phenotype-specific genes.

Conclusion
This comprehensive RNAi screening in hASC suggests that a large proportion of WAT TFs that are impacted by metabolic conditions might be important for hyperplastic adipose tissue expansion. The screen also identified JARID2 as a novel TF essential for the development of functional adipocytes.
Insulin Infusion Is Linked to Increased NPPC Expression in Muscle and Plasma C-type Natriuretic Peptide in Male Dogs
Authors: Gregory Justin M , Kraft Guillaume , Farmer Ben , Smith Marta S , LaNeve David C , Williams Phillip E , Tomasek Kelsey , Su Yan Ru , Wilson Christopher S , Thompson Mark D , Cherrington Alan D , Coate Katie C 
Published: May 2021

Abstract
The purpose of this study was to assess insulin-stimulated gene expression in canine skeletal muscle with a particular focus on NPPC, the gene that encodes C-type natriuretic peptide, a key hormonal regulator of cardiometabolic function. Four conscious canines underwent hyperinsulinemic, euglycemic clamp studies. Skeletal muscle biopsy and arterial plasma samples were collected under basal and insulin-stimulated conditions. Bulk RNA sequencing of muscle tissue was performed to identify differentially expressed genes between these 2 steady-state conditions. Our results showed that NPPC was the most highly expressed gene in skeletal muscle in response to insulin infusion, rising 4-fold between basal and insulin-stimulated conditions. In support of our RNA sequencing data, we found that raising the plasma insulin concentration 15-fold above basal elicited a 2-fold (P = 0.0001) increase in arterial plasma concentrations of N-terminal prohormone C-type natriuretic peptide. Our data suggest that insulin may play a role in stimulating secretion of C-type natriuretic peptide by skeletal muscle. In this context, C-type natriuretic peptide may act in a paracrine manner to facilitate muscle–vascular bed crosstalk and potentiate insulin-mediated vasodilation. This could serve to enhance insulin and glucose delivery, particularly in the postprandial absorptive state.
FREEDOM DOMINATION OF RABINDRANATH TAGORE’S FREEDOM POETRY: A POSTCOLONIAL APPROACH
Authors: Effendie Haris Prasetya 
Published: May 2021

War and Independence were made by all types of imperialism movement plot. A widely place which contained natural resources, and people as the tribe was victimised to create the movement. Postcolonialism tells the wealth as freed to the motherland. The motherland or kingdom of great nationalism was called and a major attend to the nationalism day to pay the national bitter history with prayer. Kingdom of human life is wealth because the years moment has been calling human to study about political relation, nationalism relation, nationalism knowledge. Case of war and how the wealth is always in upper domination are how the poetry as a literary work to reflect the soul of cultural hope and denotation in the poetry line. Wealthy is a most appropriate statue to know the social life. The nationalism knowledge is freed because the structure of greatness what human must to study is education to speak about wealth after wealthy. Descent aspect was ordered to the identity of nation, but no less to join the political descent to restart the freedom to some nation. The wisdom from any discipline to grow and refresh the freedom identity about nature and humanity. India has the nasionalism history as Indonesia when Rabindranath Tagore also talks about how the country speak about all the day long nationally wealth in his poetry to next their life. The research was a qualitative research to apply the Postcolonialism theory.
THE IMPACT OF CONCEPT MAPPING IN TEACHING SCIENCE IN MAGUINDANAO
Authors: Dalgan Norma , Camua Amour , Abellar Melda , Tabulong Analie , Unsi Alibai 
Published: May 2021

Concept mapping is a teaching strategy that helps students to organize information and facts for knowledge representation. It can be used to help students understand science subject matters and concepts. The study sougth to determine the impact of the concept mapping in teaching science among Grade 12 students in Maguindanao. Specifically, this study aims to determine the students scores in a test before and after the introduction of concept mapping strategy. Likewise, the study determined the significant improvement of the students’ scores after the concept mapping strategy in instruction. The single group pretest-posttest design was used with a detailed lesson plan. The multiple choice type of test and consists of 25 items with lesson plan was the main tool for data gathering The mean, frequencey and percentage counts were used to describe the pretest and posttest scores of the students. The t-test was used to determine the significant difference between the students’ pretest and posttest scores. The t-value was set at 0.05 levels of significance.
Findings from the study showed that there were significantly improve the scores of the pretest and posttest of the Grade 12 students in Maguindanao. This implies that the scores of the Grade 12 students improved significantly after the exposure of the concetp mapping strategy. This mean that use concetp mapping strategy increased the students’ scores.
Pratiques enseignantes Enseigner l'éducation physique en temps de pandémie
Authors: Godue-Couture Camille , Tremblay Johanne 
Published: May 2021

Cet article présente les changements apportés aux pratiques enseignantes de Johanne Tremblay, enseignante d’éducation physique et coordonnatrice départementale au cégep de Saint-Félicien, lors de la pandémie de la COVID-19. L’article fait état des différentes adaptations qu’elle a réalisées durant les sessions d’hiver et d’automne 2020. Il met en valeur le rôle et l’apport des principaux acteurs de son milieu, de même que l’importance de la collaboration durant cette période de grand bouleversement.
Phase equilibria in the Gd–Cr–Ge system at 1070 K
Authors: Konyk M. , Romaka L. , Stadnyk Yu. , Romaka V.V. , Pashkevych V. 
Published: May 2021

The isothermal section of the phase diagram of the Gd–Cr–Ge ternary system was constructed at 1070 K over the whole concentration range using X-ray diffractometry, metallography and electron microprobe (EPM) analysis. Three ternary compounds are realized in the Gd–Cr–Ge system at the temperature of annealing: Gd117Cr52Ge112 (Tb117Fe52Ge112 structure type, space group Fm-3m, Pearson symbol cF1124, a = 2.8971(6) nm), GdCr6Ge6 (SmMn6Sn6 structure type, space group P6/mmm, Pearson symbol hP16, a = 0.51797(2), c = 0.82901(4) nm) and GdCr1-хGe2 (CeNiSi2 structure type, space group Cmcm, Pearson symbol oS16, a = 0.41569(1)-0.41593(8), b = 1.60895(6)-1.60738(3), c = 0.40318(1)-0.40305(8) nm). For the GdCr1-xGe2 compound the homogeneity range was determined (x=0.73 – 0,69).
Inelastic energy loss of Ar ions scattered Al2O3 surface under grazing incidence
Authors: Ashirov A.S. , Qurbanov M.K. , Tangribergenov I.U. , Karimov M.K. , Otabaeva K.U. 
Published: May 2021

In this paper presents the investigation of the surface Al2O3 by ion scattering spectroscopy. The trajectory of small angle scattered ions calculated by the method of binary collision approximation. It was found dependence of inelastic energy loss and trajectories of scattered ions. The value of inelastic energy loss scattered ions almost depend to the angle of incidence and the geometrical parameters of surface semichannels.
Convalescent Plasma to Limit Coronavirus Associated Complications: A Proof of Concept Phase-2 Clinical Study at a designated COVID-19 hospital in Mumbai city
Authors: Pardiwalla Behram , Yadav Ajaykumar , Bhatia Ashima , Toraskar Kedar , Sharma Vijay , Shah Manishkumar , Gutte Ranjeet , Kumbhar Dhananjay , Gohel Dhaval , Narayan Preeti , Savla Honey , Nagda Nimitt , Bopardikar A. 
Published: May 2021

Objective: With few treatment options available to manage coronavirus disease 2019 (COVID-19), health systems devised strategies to manage covid-19 using repurposed drugs and revisiting older strategies, such as convalescent plasma. This study was planned to evaluate safety and efficacy of Anti-SARS-CoV-2 convalescent plasma in hospitalized subjects with COVID-19.
Method: An open label, single centre, two arm, prospective, randomised, controlled exploratory phase 2 study was conducted at a covid-19 designated center. 20 subjects (≥18 years) were admitted to hospital (screened 15 June to 27 July 2020) with confirmed moderate covid-19 (partial pressure of oxygen in arterial blood/ fraction of inspired oxygen (PaO2/FiO2) ratio between 200 mm Hg and 300 mm Hg or a respiratory rate of more than 24/min with oxygen saturation 93% or less on room air): 10 subjects were assigned to convalescent plasma with standard treatment (test arm) and 10 subjects to standard treatment only (control arm). Subjects in the test arm received either single or two doses of convalescent plasma 24 hours apart based on their clinical condition as per investigator’s discretion.
Results: Subjects in test arm showed earlier resolution of symptoms of fever, shortness of breath and cough and the mean duration for RT-PCR test turning negative was better in the test arm. One subject in the control arm progressed to severe ARDS, while none in test arm progressed to severe ARDS. There was no difference in the use of respiratory support (invasive and non-invasive ventilation) between the 2 arms. There was no mortality observed in the study and no serious adverse reaction observed with the transfusion of convalescent plasma in the study.
Conclusion: This was an exploratory proof of concept study to explore the effectiveness of convalescent plasma in COVID-19 subjects and sample size was not large enough to detect a statistically significant difference however subjects in test arm of this study showed better outcomes in few of the efficacy parameters as compared to subjects in control arm. The use of convalescent plasma transfusion was also observed to be safe.