bluuebunny/crossref_metadata_2025_split huggingface dataset:
Immunogenicity analysis of a composition of inactivated human rotavirus A strains in mice following immunisation
Authors: Kovrizhko M. V. , Kolpakova E. P. , Kolpakov D. S. , Tverdokhlebova T. I. , Kurbatov E. S. 
Published: December 2024

INTRODUCTION. Currently, rotavirus infection is prevented with live attenuated vaccines. However, international and Russian vaccination practices, as well as the physiological characteristics of paediatric patients, necessitate the development of inactivated rotavirus vaccines. Prerequisites for the development of such vaccines are the availability of virus strains capable of stable replication and the selection of optimal inactivation conditions providing for the required antigenicity and immunogenicity levels.AIM. This study aimed to evaluate and compare the characteristics of the rotavirus-specific immune response to native strains and to a composition of inactivated rotavirus A strains in a mouse model.MATERIALS AND METHODS. The study used human rotavirus A strains (RRV-4, RRV-5, RRV-6, and RRV-7), a standard rotavirus strain (SA-11 NVC 2364, National Virus Collection of the Russian Federation), and cultures of pig embryo kidney cells treated with Versene solution (SPEV) and Vero cells. Virus titration was used to determine the infectivity of the strains grown in Vero cells maintained in continuous culture. The authors monitored infected cell cultures up to the onset of the cytopathic effect, calculated the 50% tissue culture infectious dose (TCID50) by the Kärber method modified by Ashmarin, and expressed the results as log10 TCID50/mL. Virus strains were inactivated with formaldehyde. To evaluate immuno­genicity, outbred white mice were immunised with native strains and the composition of inactivated strains (RRV-4, RRV-5, RRV-6, and RRV-7). After immunisation, blood was taken from the animals, and the serum titre of rotavirus A antibodies was determined by indirect heterogeneous enzyme immunoassay.RESULTS. The infectivity of the rotavirus strains adapted to Vero cells ranged from 8.9 to 7.9 log10 TCID50/mL. When selecting inactivation conditions, the authors showed that inactiva­tion occurred at a temperature of 37 °C and a formaldehyde concentration of 0.05–0.025% (depending on the duration of treatment). The antigenicity analysis demonstrated that the antigen titre of the inactivated strain composition (1:16) was lower than that of native strains (1:32–1:64). The authors demonstrated comparability of immunogenicity profiles of the inacti­vated strain composition and native strains in mice.CONCLUSIONS. The study generated candidate rotavirus A strains that exhibited stable replica­tion in continuous cultures of Vero cells. The authors selected optimal inactivation conditions for these rotavirus strains and developed an inactivated strain composition showing antigenicity and immunogenicity. The presented data suggest that the composition of inactivated rotavirus A strains can be considered as a basis for further development of an inactivated rotavirus vaccine.
Potency evaluation of erythropoietin products: current status
Authors: Gaiderova L. A. , Alpatova N. A. , Lysikova S. L. , Guskov A. M. , Golovinskaya O. V. 
Published: December 2024

INTRODUCTION. Given the widespread clinical use of recombinant human erythropoietin (rhEPO) products from different manufacturers, potency assays should ensure that patients receive comparable doses of rhEPO across medicinal products. The harmonisation of approaches to potency testing requires the use of pharmacopoeial bioassays and appropriate international/pharmacopoeial reference standards (RSs).AIM. This study aimed to summarise information on pharmacopoeial requirements, relevant RSs, and bioassays (in vivo and in vitro) for the assessment of rhEPO potency, as well as to analyse the pharmacopoeial compliance of manufacturers’ specifications for rhEPO products authorised in Russia.DISCUSSION. This article presents information on the molecular structure of erythropoietin. The glycosylation profile of erythropoietin not only accounts for most of the differences in the half-life and biodegradation rate but also significantly influences the potency of rhEPO products. The authors outlined the pharmacopoeial requirements for potency assays in vivo, summarised the information on RSs for potency determination, characterised the development of potency assays in vitro, and studied the possibility of including in vitro assays in pharmacopoeias. The analysis showed that some potency assays used for rhEPO products manufactured in Russia did not comply with the requirements of the State Pharmacopoeia of the Russian Federation.CONCLUSIONS. The study identified the need to develop and certify a national pharmacopoeial RS for the potency of rhEPO in order to satisfy the demands of Russian manufacturers in the context of import substitution. To implement adequate 3R-compliant methods for rhEPO quality assessment, it is necessary to harmonise approaches to potency testing of rhEPO products and develop a consolidated document governing such testing.
Determination of the genetic dimension of Pseudomonas stutzeri which of nitrogen-fixation bacteria in a random PCR method
Authors: Fawz Abdul Al-Salam Kalil Al-Saffar  
Published: December 2024

Bacteria play an important role in fixing atmospheric nitrogen and converting it into compounds that can be used by plants. Due to the importance of this topic, this type of bacteria was chosen, and its genetic dimension was studied. The nitrogen-fixing strain of Pseudomonas stutzeri, or those that have already been positively identified, should be collected from the wild or from laboratory cultures. Pseudomonas stutzeri strain was collected and their genomic DNA was extracted. This can be done using regular methods for extracting DNA. The purpose of this research is to generate a diverse panel of PCR amplification primers. Primers chosen at random are short sequences of nucleotides that can bind to various locations in the bacterial genome. These primers can be used to initiate polymerase chain reaction (RPCR) amplification at a high number of random positions within the DNA, allowing researchers to probe a wide range of genes. Fifteen different bundles, ranging in size from 200 to 3000 base pairs, were recorded. Thirteen differential bundles of 350, 425, 600, 650, 700, 800, 950, 1000, 1300, 1400, 1500, 1700, and 3000 base pairs are featured by the inclusion of two public bundles of 200 and 2000. Fifteen different bundles, ranging in size from 200 to 3000 base pairs, were recorded. Thirteen differential bundles of 350, 425, 600, 650, 700, 800, 950, 1000, 1300, 1400, 1500, 1700, and 3000 base pairs are featured by the inclusion of two public bundles of 200 and 2000.
Tinjauan Yuridis Pembuktian Elektronik dalam Perkara Tindak Pidana Penipuan Online
Authors: Siregar Zulkifli Anwar , Rafiqi Rafiqi , Sitorus Nanang Tomi 
Published: December 2024

The purpose of this writing is to be able to review and analyze electronic information evidence of criminal acts of online fraud from the perspective of criminal procedural law in Indonesia. The method used in this writing is to use a normative juridical method approach. The problems discussed in this paper are how electronic evidence is used to prove criminal acts of fraud through online media and how electronic evidence is used for online fraud crimes based on Law no. 16 of 2019 concerning Amendments to Law no. 11 of 2008 concerning Information and electronic transactions, therefore the judge's task in his authority as a law enforcer is to interpret or interpret electronic evidence as an extension of documentary evidence or instructions which are valid evidence according to Article 184 of the Criminal Procedure Code, as regulated in Article 5 paragraphs (1) and (2) Law no. 16 of 2019 concerning Amendments to Law Number 11 of 2008 concerning Information and Electronic Transactions which regulates evidence, namely electronic information, electronic documents and printouts, as valid evidence and the expansion of evidence in criminal procedural law relating to acts criminal penalties regulated in the electronic information and transactions law. Apart from that, to assist in interpreting and interpreting electronic evidence, judges need expert testimony in the evidentiary process. So, electronic information evidence is an extension of expert testimony and guidance evidence in accordance with Article 184 of the Criminal Procedure Code, expansion of legal evidence based on criminal procedural law. Furthermore, in Article 5 paragraph (3) of Law no. 16 of 2019 concerning Amendments to Law no. 11 of 2008 concerning Information and Electronic Transactions, through a negative legal proof system, that proof is not based only on legal regulations, but also on the judge's belief, so the judge also needs expert information to find out the real facts regarding electronic evidence, p. This relates to article 1 points 1 and 4 of Law no. 16 of 2019 concerning Amendments to Law number 11 of 2008 concerning Information and Electronic Transactions.
A Comparative Study of the Adjustment of Employees Working in the Police Department
Authors: Rathava Aminabahen Kunvarsing , Savalia Jagdishchandra K. 
Published: December 2024

The objective of this research study was to conduct a comparative study of the adjustment of employees working in the police department. The sample for the study was selected based on the research objectives. Female and male employees working in the Chhota Udepur district of the Vadodara range of the police department were chosen as the sample for this research study. Employees with at least five years of work experience and aged above 25 years were selected as the sample. The study included 103 female and 197 male employees as participants. To measure adjustment, a questionnaire developed by Dr. Pramod Kumar was used. To compare the mental health of employees working in the police department, the 't-test' ratio was applied at the 0.05 level to examine the significance of the means. The findings revealed that there was no significant difference in adjustment between the groups of male and female employees. Both male and female employees were found to have similar levels of adjustment.
Abstract in Gujarati Language: આ સંશોધન અભ્યાસનો હેતુ પોલીસ વિભાગમાં કામ કરતા કર્મચારીઓના સમાયોજનનો તુલનાત્મક અભ્યાસ કર્યો હતો. આ સંશોધન અભ્યાસમાં સંશોધન હેતુઓને ધ્યાનમાં રાખી નમૂનો પસંદ કરવામાં આવ્યો હતો. આ સંશોધન અભ્યાસમાં પોલીસ વિભાગમાં વડોદરા રેન્જના છોટા ઉદેપુર જિલ્લામાં કામ કરતી મહિલા અને પુરુષ કર્મચારીઓને નમૂના તરીકે પસંદ કરવામાં આવ્યા હતા. આ સંશોધન અભ્યાસમાં પાંચ વર્ષનો નોકરીનો અનુભવ પૂરો થયો હોય તેવા અને 25 વર્ષથી વધારે ઉંમર ધરાવતા કર્મચારીઓને નમૂના તરીકે પસંદ કરવામાં આવ્યા હતા. આ સંશોધન અભ્યાસમાં 103 મહિલાઓ અને 197 પુરુષ કર્મચારીઓને નમૂના તરીકે પસંદ કરવામાં આવ્યા હતા. આ સંશોધન અભ્યાસમાં સમાયોજનના માપન માટે ડૉ. પ્રમોદકુમાર દ્વારા રચિત પ્રશ્નાવલિનો ઉપયોગ કરવામાં આવ્યો હતો. પોલીસ વિભાગમાં કામ કરતા કર્મચારીઓના સમાયોજન તુલના કરવા માટે 0.05 કક્ષાએ ‘t’ ટેસ્ટ રેશિયો લાગુ પાડી મધ્યકોની સાર્થકતા ચકાસવામાં આવી હતી. જેનું તારણ આ પ્રમાણે જોવા મળ્યું હતું. પુરૂષો અને મહિલાઓના જૂથો વચ્ચે સમાયોજનમાં અર્થપૂર્ણ તફાવત જોવા મળ્યો ન હતો. પુરૂષો અને મહિલાઓ સમાયોજનમાં સમાન જોવા મળ્યા હતા.
Keywords: પોલીસ વિભાગ, કર્મચારીઓનું સમાયોજન, તુલનાત્મક અભ્યાસ
Pengaruh Layanan Konseling Kelompok Terhadap Konsep Diri Peserta Didik SMP
Authors: Indy Rahma Aulia  , Dwi Dasalinda  , Eka Heriyani  , Haning Tri Widiastuti  
Published: December 2024

Penelitian ini dilatarbelakangi oleh permasalahan yang muncul pada peserta didik yang berkaitan dengan konsep diri. Berdasarkan permasalahan tersebut peneliti melakukan penelitian yang bertujuan untuk mengetahui pengaruh konseling kelompok dalam meningkatkan konsep diri peserta didik di SMPN 9 Tambun Selatan. Penelitian ini menggunakan metode kuantitatif pra-eksperimen model one group pre-test post-test. Berdasarkan analisis statistik didapatkan nilai sig. sebesar 0.001 untuk mendapatkan titik ‘t’ dengan taraf signifikansi 5%. df=8-1=7, t0=5,33 dan tt= 2,36. Berdasarkan hasil uji analisis membuktikan bahwa Konseling Kelompok berpengaruh signifikan dalam meningkatkan konsep diri peserta didik di SMPN 9 Tambun Selatan. Penelitian ini penting dilakukan untuk meningkatkan konsep diri peserta didik, karena konsep diri positif mampu memberikan dampak baik dalam bidang akademik maupun sosial.
Kata kunci: konseling kelompok, konsep diri, peserta didik
Smartphone Distraction dan Driving Behavior Pada Driver Ojek Online
Authors: Muhammad Alvin Septiandy  , Jusuf Tjahjo Purnomo  
Published: December 2024

Perilaku mengemudi yang berisiko dapat berakibat pada kondisi yang membahayakan seperti kecelakaan lalu lintas. Salah satu faktor yang menyebabkan kecelakaan di jalan adalah perilaku mengemudi ojek online yang lebih fokus pada smartphone dari pada ke jalan. Tujuan penelitian ini adalah menganalisis hubungan smartphone distraction terhadap driving behavior pada driver ojek online. Metode penelitian yang digunakan adalah pendekatan kuantitatif menggunakan teknik purposive sampling. Sampel yang diambil sebanyak 129 yang merupakan driver ojek online di Salatiga. Pengumpulan data menggunakan skala smartphone distraction dan skala driving behavior. Analisis data kuantitatif meliputi uji korelasi product moment. Hasil penelitian menunjukkan terdapat hasil korelasi positif sebesar 0,537 dengan sig=0,000 (p<0,005) yang dapat diartikan bahwa terdapat hubungan positif antara smartphone distraction dengan driving behavior pada driver ojek online. Dampak dari penelitian ini memberikan gambaran agar driver ojek online tidak fokus pada smartphone saat berkendara, karena membahayakan konsentrasi berkendara.
Kata kunci: distraksi ponsel, perilaku mengemudi, pengemudi ojek online
ФОРМУВАННЯ МОВНО-МОВЛЕННЄВОЇ КОМПЕТЕНТНОСТІ ЗДОБУВАЧІВ ПОЧАТКОВОЇ ОСВІТИ: МЕТОДИЧНІ АСПЕКТИ
Authors: Собко Валентина 
Published: December 2024

Статтю присвячено розгляду проблеми реалізації мети мовно-літературної освітньої галузі (українська мова і література) у початковій мовній освіті, зокрема методичних аспектів формування мовно-мовленнєвої компетентності молодших школярів як важливого складника ключової компетентності «вільне володіння державною мовою».
У роботі розкрито сутність предметних (мовна і мовленнєва) компетентностей у структурі комунікативної; проаналізовано суть поняття «мовно-мовленнєва компетентність»; визначається зміст роботи над мовно-мовленнєвою компетентністю.
Обґрунтовуються лінгводидактичні можливості її формування у процесі дослідження мовних явищ (лексичних, лексико-граматичних, синтаксичних, текстологічних) у двох аспектах: засвоєння учнями доступного й необхідного обсягу мовних знань та розвиток навичок мовленнєвої діяльності.
Зазначено, що вивчення мовних одиниць і явищ у початкових класах має особливе значення у процесі формування мовно-мовленнєвої компетентності молодшого школяра. Це насамперед пояснюється метою уроків української мови в початковій ланці освіти – допомогти молодшим школярам оволодіти нормами граматичної структури української мови, збагатити мовлення різноманітними формами і способами висловлення думки, піднести рівень розвитку зв’язного мовлення.
Автором запропоновано вправи і завдання, метою яких є опанування учнями найважливіших функційних складників мовної системи (слово, речення, текст), використання молодшими школярами мовних явищ у процесі мовлення відповідно до мети й умов спілкування; представлено види мовних і мовленнєвих вправ, зорієнтованих на збагачення словника здобувачів початкової освіти, формування граматичних і текстологічних умінь, прагнення вдосконалювати своє мовлення.
Приділено увагу висвітленню продуктивності поєднання в системі навчання української мови змістового і процесуального блоків, що передбачає формування мовно-мовленнєвої компетентності у процесі дослідження мовних та мовленнєвих явищ.
Подано рекомендації щодо використання інтерактивних методів навчання, які дають можливість вирішувати такі завдання: активно включити кожного учня в процес засвоєння навчального матеріалу; підвищити мотивацію до навчання; створювати атмосферу співробітництва, взаємодії; розвивати уміння успішного спілкування (вміти слухати і чути один одного, вибудовувати правильно діалог).
Трансформація судової системи та її вплив на правовий статус селян Правобережної України наприкінці 18 - на початку 19 ст.
Authors: Шевчук Андрій 
Published: December 2024

The purpose of the article is a comprehensive analysis of the changes in the judicial system of the Right-Bank Ukraine after its incorporation into the Russian Empire and their impact on the legal status of the serfdom in the late 18th and early 19th centuries. The methodological basis of the study is the principles of historicism and scientific objectivity, as well as critical, systematic and comparative analysis of sources. The application of these methods and principles made it possible to study the transformation of the judicial system and the legal status of peasants in dynamics and in aggregate, taking into account all contradictory factors. The use of various methods made it possible to trace, on the example of the Right-Bank Ukraine, the impact of Russian imperial legislation on the events and processes which regulated the activities of judicial institutions and determined the legal status of landed peasants. Conclusions. The incorporation of the Right-Bank Ukraine into the Russian Empire led to significant changes in the judicial system of the region and affected the legal status of serfs. There was a transition from the manorial (patrimonial) court to the imperial court system, where criminal cases of serfs for medium and serious crimes were considered by state judicial institutions. The introduction of the “Statute on the Administration of the Provinces of the Russian Empire” in 1775 and further reforms by Paul I formed a new structure of judiciary on the Right Bank. An analysis of court practice shows that serfs were subject to trial for a wide range of criminal offenses, with a tendency to mitigate punishments compared to the norms of the Lithuanian statutes and imperial justice. The supreme power sought to establish control over the proceedings against serfs, limiting the power of landlords in criminal cases to minor offenses. The judicial system became a tool for integrating the Right Bank into the imperial space, but at the same time retained certain elements of local specificity.
THE NEED FOR PROSTHETIC TREATMENT WITH DIFFERENT DENTURE DESIGNS FOR RESIDENTS OF ODESA AND THE ODESA REGION
Authors: Gorbunov A.A. , Zahradska O.L. 
Published: December 2024

Objective. The aim of this study is to analyze the need for orthopedic treatment with removable dental prosthetic constructions among the residents of Odesa and the Odesa region based on a retrospective analysis of outpatient dental patient records.
Materials and Methods. This research was conducted using outpatient records (form 043/o) of patients who sought dental treatment with removable orthopedic constructions at healthcare institutions in the Odesa region and city dental departments from 2019 to 2023.
Results. The analysis examined the proportion of removable prostheses relative to the total number of prostheses manufactured. From 2019 to 2023, 5,439 individuals (38.1%) in the Odesa region received removable dental prostheses, while 8,272 (61.9%) were from Odesa. A total of 95,237 prostheses were produced, with 65.3% (62,182) made in Odesa. In 2020, 33,488 prostheses were manufactured; in 2021, 42,026; and in 2022, 19,723. The need for prosthetic treatment in Odesa is estimated at 57% (570 individuals per 1,000 population). Patient visits were recorded as follows: 176 individuals in 2020, 212 in 2021, and 182 in 2022. In 2022, the number of visits decreased by 10,428 individuals (15.5%) compared to 2020, and by 3,001 individuals (17.95%) compared to 2021, indicating a decline in the accessibility of dental services.
Conclusion. The study conducted in the Odesa region (2019-2023) revealed a high demand for orthopedic treatment, particularly for removable dental prostheses. 38.1% of patients received prostheses, with 61.9% coming from Odesa. A decrease in patient visits was observed in 2022 that was likely due to the consequences of the COVID-19 pandemic and economic difficulties. The results highlight the need for monitoring the population's demand for dental services and adapting strategies to improve accessibility and quality of treatment.
Catechin Isolates from Gambir (Uncaria gambir Roxb) Maintain Glucose Homeostasis in Diabetic Model Rat
Authors: Rita Rauza Sukma , Yetti Husna , Santoso Putra , Afriani Nita 
Published: December 2024

The prevalence of diabetes mellitus is rising globally. Oxidative stress, which can result from hyperglycemia in diabetes, might have negative consequences. An antioxidant is needed to prevent hyperglycemia. Cathechin isolates are derived from gambir, which has many antioxidants. This study examines catechin isolates from gambir (Uncaria gambir Roxb.) effect on glucose homeostasis in rats induced by alloxan. For this experiment, 35 male rats were employed. Male rats were given alloxan (150 mg/BW, IP), and after 72 hours, blood glucose levels were assessed. If blood glucose levels exceeded 200 mg/dl, three oral catechin isolates were administered (T1=10 mg/kg/day, T2=20 mg/kg/day, and T3=40 mg/kg/day). Following blood collection on the experiment's last day, fasting blood glucose, glucagon and insulin levels were measured. Catechin isolates decreased blood glucose levels in all treatment groups compared to the positive control group (T1 = 150.750 ± 14.359 mg/dl; T2 = 159.750 ± 15.434 mg/dl, and T3 = 153.375 ± 20.207 mg/dl vs 385 ± 60.989 mg/dl) significantly (p value-0.05). A decrease in glucagon serum level was also observed in the treatment group vs positive control (T1: 193.855 ± 36.009 pg/ml, T2 = 286.689 ± 20.313 pg/ml, and T3 = 319.462 ± 30.060 pg/ml vs 529.825 ± 74.279 pg/ml), significantly. Catechine isolates in the T3 group showed an increase in insulin serum level compared to the positive control group significantly (T3 = 216.640 ± 38.230 µIU/ml vs 69.833 ± 3.071 µIU/ml). In conclusion, catechin isolates from gambir decreased blood glucose levels by reducing glucagon and increasing insulin serum levels.
Survey of Association Between Indoor CO and CO2 Levels and Symptoms of Sick Building Syndrome Among Employees at Alang-Alang Lebar and Plaju Health Centers
Authors: Delviana  , Fitri Agita Diora , Mariana  
Published: December 2024

Poor air quality and exceptionally high CO and CO2 pollutants can be contributing factors to Sick Building Syndrome (SBS). This study evaluated the relationship between individual factors and CO and CO2 levels with Sick Building Syndrome (SBS) symptoms in health center employees. 74 respondents work in the room of Alang-Alang Lebar and Plaju Health Center. Then, the respondents will fill out a questionnaire related to SBS symptoms. The rooms in the selected health centers will be measured for CO and CO2 levels. The results of filling out the questionnaire will be analyzed to determine whether SBS or not, and the results of measuring CO and CO2 levels will also be categorized as normal or not. Univariate and bivariate analyses related to individual employee factors, SBS symptoms, and CO and CO2. The results of 74 respondents showed that 36 respondents (48.6%) experienced SBS. The highest SBS symptoms were red, itchy, dry, or watery eyes (75%), and symptoms of dizziness or headache (71.4%). The highest CO level measurement result was seven ppm, the lowest was six ppm, while the highest CO2 level was 561 ppm, and the lowest was 498 ppm. These measurement results are still within the normal limits of the standards set. Bivariate test results showed that individual factors, CO and CO2, are not significantly related to SBS symptoms (p > 0.05).