bluuebunny/crossref_metadata_2025_split huggingface dataset:
Exploring the relation between mortality and left ventricular structure and function in stable hemodialysis treated patients, a longitudinal multicenter cohort study
Authors: Chisavu Lazar A. , Apostol Adrian , Pop Gheorghe N. , Ivan Viviana , Schiller Oana , Bob Flaviu , Marc Luciana , Mihaescu Adelina , Gadalean Florica , Grosu Iulia , Timar Bogdan , Schiller Adalbert 
Published: June 2021

AbstractLeft ventricular (LV) structure and function anomalies are frequent during the CKD continuum and are associated with increased risk of mortality. Cross section and longitudinal ultrasound data are available for advanced CKD and transition to ESKD. Less information is available about LV changes during stable, long-term hemodialysis (HD) treatment. All stable HD patients from 9 HD centers (1034 patients, 671 males, age 58.71 ± 12.94 years) have been enrolled in January 2015. The cohort was followed-up for 4 years, kidney transplantation or death. Yearly, two-dimensional and M-mode continuous and Pulse Doppler echocardiography were performed. During the follow-up, the prevalence of cardiovascular comorbidities significantly increased (p < 0.0001), coronary artery disease (CAD) from 73.5 to 88.8%, peripheral artery disease (PAD) from 29 to 40.9%, cerebral vascular disease (CVD) from 20.4 to 30.8%, heart valves calcification (VC) from 65.6 to 89.3% and left ventricular hypertrophy (LVH) from 67.6 to 76.5%. The mortality risk increased with the presence of CAD (1.59-fold), PAD (1.61-fold), CVD (1.59-fold), and VC (1.77-fold). Mortality risk was increased in those with LVEF < 50% (LVEF 40–49% 1.5-fold and LVEF < 40% 2.3 fold). Among the survivors of the first year, LVEF varied (> 5% decrease, > 5% increase and ± 5% variations). More than 5% increase of LVEF was associated with higher mortality risk (crude 1.5-fold, adjusted 1.43-fold) compared to stationary EF (p = 0.001). Cardiovascular disease progresses during stable long-term HD therapy and increases mortality risk. HF becomes highly prevalent but only HF with decreased LVEF < 50% is associated with increased risk of mortality.
Application of the random forest algorithm to Streptococcus pyogenes response regulator allele variation: from machine learning to evolutionary models
Authors: Buckley Sean J. , Harvey Robert J. , Shan Zack 
Published: June 2021

AbstractGroup A Streptococcus (GAS) is a globally significant bacterial pathogen. The GAS genotyping gold standard characterises the nucleotide variation of emm, which encodes a surface-exposed protein that is recombinogenic and under immune-based selection pressure. Within a supervised learning methodology, we tested three random forest (RF) algorithms (Guided, Ordinary, and Regularized) and 53 GAS response regulator (RR) allele types to infer six genomic traits (emm-type, emm-subtype, tissue and country of sample, clinical outcomes, and isolate invasiveness). The Guided, Ordinary, and Regularized RF classifiers inferred the emm-type with accuracies of 96.7%, 95.7%, and 95.2%, using ten, three, and four RR alleles in the feature set, respectively. Notably, we inferred the emm-type with 93.7% accuracy using only mga2 and lrp. We demonstrated a utility for inferring emm-subtype (89.9%), country (88.6%), invasiveness (84.7%), but not clinical (56.9%), or tissue (56.4%), which is consistent with the complexity of GAS pathophysiology. We identified a novel cell wall-spanning domain (SF5), and proposed evolutionary pathways depicting the ‘contrariwise’ and ‘likewise’ chimeric deletion-fusion of emm and enn. We identified an intermediate strain, which provides evidence of the time-dependent excision of mga regulon genes. Overall, our workflow advances the understanding of the GAS mga regulon and its plasticity.
Analysis of spatiotemporal changes of agricultural land after the Second World War in Czechia
Authors: Zelinka Vít , Zacharová Johana , Skaloš Jan 
Published: June 2021

AbstractThe term Sudetenland refers to large regions of the former Czechoslovakia that had been dominated by Germans. German population was expelled directly after the Second World War, between 1945 and 1947. Almost three million people left large areas in less than two years. This population change led to a break in the relationship between the people and the landscape. The aim of the study is to compare the trajectories of these changes in agricultural landscapes in lower and higher altitudes, both in depopulated areas and areas with preserved populations. This study included ten sites in the region of Northern Bohemia in Czechia (18,000 ha in total). Five of these sites represent depopulated areas, and the other five areas where populations remained preserved. Changes in the landscape were assessed through a bi-temporal analysis of land use change by using aerial photograph data from time hoirzons of 2018 and 1953. Land use changes from the 1950s to the present are corroborated in the studied depopulated and preserved areas mainly by the trajectory of agricultural land to forest. The results prove that both population displacement and landscape type are important factors that affect landscape changes, especially in agricultural landscapes.
Effects of transcranial direct current stimulation combined with listening to preferred music on memory in older adults
Authors: Chow Ricky , Noly-Gandon Alix , Moussard Aline , Ryan Jennifer D. , Alain Claude 
Published: June 2021

AbstractListening to autobiographically-salient music (i.e., music evoking personal memories from the past), and transcranial direct current stimulation (tDCS) have each been suggested to temporarily improve older adults’ subsequent performance on memory tasks. Limited research has investigated the effects of combining both tDCS and music listening together on cognition. The present study examined whether anodal tDCS stimulation over the left dorsolateral prefrontal cortex (2 mA, 20 min) with concurrent listening to autobiographically-salient music amplified subsequent changes in working memory and recognition memory in older adults than either tDCS or music listening alone. In a randomized sham-controlled crossover study, 14 healthy older adults (64–81 years) participated in three neurostimulation conditions: tDCS with music listening (tDCS + Music), tDCS in silence (tDCS-only), or sham-tDCS with music listening (Sham + Music), each separated by at least a week. Working memory was assessed pre- and post-stimulation using a digit span task, and recognition memory was assessed post-stimulation using an auditory word recognition task (WRT) during which electroencephalography (EEG) was recorded. Performance on the backwards digit span showed improvement in tDCS + Music, but not in tDCS-only or Sham + Music conditions. Although no differences in behavioural performance were observed in the auditory WRT, changes in neural correlates underlying recognition memory were observed following tDCS + Music compared to Sham + Music. Findings suggest listening to autobiographically-salient music may amplify the effects of tDCS for working memory, and highlight the potential utility of neurostimulation combined with personalized music to improve cognitive performance in the aging population.
Two-sided matching based on I-BTM and LSGDM applied to high-level overseas talent and job fit problems
Authors: Yang Qing , You Xinshang , Zhang Yiye 
Published: June 2021

AbstractWith the increasing number of overseas talent tasks in China, overseas talent and job fit are significant issues that aim to improve the utilization of this key human resource. Many studies based on fuzzy sets have been conducted on this topic. Among the many fuzzy set methods, intuitionistic fuzzy sets are usually utilized to express and handle the evaluation information. In recent years, various intuitionistic fuzzy decision-making methods have been rapidly developed and used to solve evaluation problems, but none of them can be used to solve the person-job fit problem with intuitionistic best-worst method (BWM) and TOPSIS methods considering large-scale group decision making (LSGDM) and evaluator social network relations (SNRs). Therefore, to solve problems of intuitionistic fuzzy information analysis and the LSGDM for high-level overseas talent and job fit, we construct a new hybrid two-sided matching method named I-BTM and an LSGDM method considering SNRs. On the one hand, to express the decision-making information more objectively and reasonably, we combine the BWM and TOPSIS in an intuitionistic environment. Additionally, we develop the LSGDM with optimized computer algorithms, where the evaluators’ attitudes are expressed by hesitant fuzzy language. Finally, we build a model of high-level overseas talent and job fit and establish a mutual criteria system that is applied to a case study to illustrate the efficiency and reasonableness of the model.
A novel automated SARS-CoV-2 saliva PCR test protects a global asymptomatic workforce
Authors: Carter Nikki , Clausen Maryam , Halpin Rebecca A. , Blackmore Colin , Cai Kang , Delpuech Oona , Kohlmann Alexander , Magnusson Otto , March Ruth , O’Neill Daniel , Prakash Kasthuri , Sherwood James , Sundin Tabetha , Swift Jason , Tarakameh Azar , Wijdicks Marilou , Wise Daniel , Fidock Mark 
Published: June 2021

AbstractRegular PCR testing of nasopharyngeal swabs from symptomatic individuals for SARS-CoV-2 virus has become the established method by which health services are managing the COVID-19 pandemic. Businesses such as AstraZeneca have also prioritised voluntary asymptomatic testing to keep workplaces safe and maintain supply of essential medicines to patients. We describe the development of an internal automated SARS-CoV-2 testing programme including the transformative introduction of saliva as an alternative sample type.
Assessment with clinical data of a coupled bio-hemodynamics numerical model to predict leukocyte adhesion in coronary arteries
Authors: Ciri Umberto , Bennett Ruth L. , Bhui Rita , Molony David S. , Samady Habib , Meyer Clark A. , Hayenga Heather N. , Leonardi Stefano 
Published: June 2021

AbstractNumerical simulations of coupled hemodynamics and leukocyte transport and adhesion inside coronary arteries have been performed. Realistic artery geometries have been obtained for a set of four patients from intravascular ultrasound and angiography images. The numerical model computes unsteady three-dimensional blood hemodynamics and leukocyte concentration in the blood. Wall-shear stress dependent leukocyte adhesion is also computed through agent-based modeling rules, fully coupled to the hemodynamics and leukocyte transport. Numerical results have a good correlation with clinical data. Regions where high adhesion is predicted by the simulations coincide to a good approximation with artery segments presenting plaque increase, as documented by clinical data from baseline and six-month follow-up exam of the same artery. In addition, it is observed that the artery geometry and, in particular, the tortuosity of the centerline are a primary factor in determining the spatial distribution of wall-shear stress, and of the resulting leukocyte adhesion patterns. Although further work is required to overcome the limitations of the present model and ultimately quantify plaque growth in the simulations, these results are encouraging towards establishing a predictive methodology for atherosclerosis progress.
Premature ventricular contraction increases the risk of heart failure and ventricular tachyarrhythmia
Authors: Kim Yun Gi , Choi Yun Young , Han Kyung-Do , Min Kyoung Jin , Choi Ha Young , Shim Jaemin , Choi Jong-Il , Kim Young-Hoon 
Published: June 2021

AbstractPremature ventricular contraction (PVC), a common arrhythmia affecting 1–2% of the general population, has been considered to have a benign clinical course. However, people with PVC often develop heart failure and ventricular arrhythmias such as ventricular tachycardia. We aimed to clarify the risk of heart failure and lethal ventricular arrhythmias in people with PVC. The Korean National Health Insurance Service database was used for this study. People who underwent nationwide health check-ups in 2009 were enrolled in this study and clinical follow-up data until December 2018 were analyzed. Newly diagnosed PVC in 2009 (≥ 1 inpatient or outpatient claim) were identified and cumulative incidence of heart failure (≥ 1 inpatient claim) and ventricular arrhythmias (≥ 1 inpatient or outpatient claim) were compared. A total of 4515 people were first diagnosed with PVC in 2009 among 9,743,582 people without prior history of PVC, heart failure, or ventricular arrhythmias. People with newly diagnosed PVC in 2009 had a significantly higher incidence of heart failure compared to those without PVC [adjusted hazard ratio (HR)  1.371; 95% confidence interval (CI)  1.177–1.598; p  < 0.001]. Significant interaction was observed between age and PVC with young age people at greater risk of developing heart failure for having PVC. The incidence of ventricular arrhythmia was also significantly increased in people with PVC (HR  5.588; 95% CI  4.553–6.859; p  < 0.001). Age and chronic kidney disease had significant interactions with PVC. In conclusion, the incidence of heart failure and ventricular arrhythmia was significantly increased in people with PVC. Outpatient follow-up of people with PVC can be helpful to detect early signs of heart failure or advanced forms of ventricular arrhythmia.
Individual and community level factors associated with unintended pregnancy among pregnant women in Ethiopia
Authors: Zeleke Liknaw Bewket , Alemu Addisu Alehegn , Kassahun Eskeziaw Abebe , Aynalem Bewket Yeserah , Hassen Hamid Yimam , Kassa Getachew Mullu 
Published: June 2021

AbstractUnintended pregnancy is among the major challenges of public health and a major reproductive health issue, due to its implications on the health, economic and social life of a woman and her family mainly in low and middle-income countries, particularly sub-Saharan Africa. The study aimed to assess unintended pregnancy and associated factors among pregnant women in Ethiopia using multilevel analysis from the EDHS 2016. We used the data from the 2016 Ethiopian Demographic and Health Survey, comprised of 1122 pregnant women. The prevalence of unintended pregnancy was determined through descriptive statistics and multilevel logistic regression was performed to identify factors associated with unintended pregnancy. Variables with a p-value < 0.05 in the selected model were considered as significantly associated and an adjusted odds ratio was used to determine the strength and direction of the association. The prevalence of unintended pregnancy was 29.7% (CI 27.0%, 32.4%), of which 20.4% were mistimed and 9.3% unwanted. Being multi-para and fertility preference to have no more child were associated with a higher risk of unintended pregnancy whereas husbands' polygamy relation, having no women autonomy, and living in Afar and Somali regions showed a less likely risk of experiencing an unintended pregnancy. This study showed that the proportion of women who experienced unintended pregnancy is considerably high. Parity, fertility preference, polygamy relation, women autonomy, and region were identified factors associated with unintended pregnancy. Therefore, policymakers at all levels, reproductive health experts, and concerned organizations should emphasize minimizing unintended pregnancy targeting the regional variation at large. Researchers have to explore the regional variations through a qualitative study.
Learning curve evaluation upskilling retinal imaging using smartphones
Authors: Jansen Linus G. , Shah Payal , Wabbels Bettina , Holz Frank G. , Finger Robert P. , Wintergerst Maximilian W. M. 
Published: June 2021

AbstractSmartphone-based fundus imaging (SBFI) is a low-cost approach for screening of various ophthalmic diseases and particularly suited to resource limited settings. Thus, we assessed how best to upskill alternative healthcare cadres in SBFI and whether quality of obtained images is comparable to ophthalmologists. Ophthalmic assistants and ophthalmologists received a standardized training to SBFI (Heine iC2 combined with an iPhone 6) and 10 training examinations for capturing central retinal images. Examination time, total number of images, image alignment, usable field-of-view, and image quality (sharpness/focus, reflex artifacts, contrast/illumination) were analyzed. Thirty examiners (14 ophthalmic assistants and 16 ophthalmologists) and 14 volunteer test subjects were included. Mean examination time (1st and 10th training, respectively: 2.17 ± 1.54 and 0.56 ± 0.51 min, p < .0001), usable field-of-view (92 ± 16% and 98 ± 6.0%, p = .003) and image quality in terms of sharpness/focus (p = .002) improved by the training. Examination time was significantly shorter for ophthalmologists compared to ophthalmic assistants (10th training: 0.35 ± 0.21 and 0.79 ± 0.65 min, p = .011), but there was no significant difference in usable field-of-view and image quality. This study demonstrates the high learnability of SBFI with a relatively short training and mostly comparable results across healthcare cadres. The results will aid implementing and planning further SBFI field studies.
Influence of lens opacities and cataract severity on quantitative fundus autofluorescence as a secondary outcome of a randomized clinical trial
Authors: Reiter Gregor S. , Schwarzenbacher Luca , Schartmüller Daniel , Röggla Veronika , Leydolt Christina , Menapace Rupert , Schmidt-Erfurth Ursula , Sacu Stefan 
Published: June 2021

AbstractThe aim of this study is to investigate the impact of age-related lens opacities and advanced cataract, quantified by LOCS III grading, on quantitative autofluorescence (qAF) measurements in patients before and after cataract surgery. Images from a randomized controlled trial evaluating the impact of femtosecond-laser assisted cataract surgery (FLACS) on retinal thickness were analyzed post-hoc. One-hundred and twenty eyes from 60 consecutive patients with age-related cataract were included and assessed with qAF and optical coherence tomography (OCT) before, 1, 3 and 6 weeks after cataract surgery (randomized 1:1 to FLACS or phacoemulsification). LOCS III grading was performed before surgery. Pre- to post-surgical qAF values, as well as the impact of LOCS III gradings, surgery technique, gender, axial length and age on post-surgery qAF values was investigated using generalized linear mixed models. For this analysis, 106 eyes from 53 patients were usable. No difference in qAF was found between FLACS and phacoemulsification (p > 0.05) and results were pooled for the total cohort. Mean pre-surgical qAF was 89.45 ± 44.9 qAF units, with a significant mean increase of 178.4–191.6% after surgery (p < 0.001). No significant difference was found between the three follow-up visits after surgery (p > 0.05). Higher LOCS III cortical opacity quantifications were associated with a significantly greater increase in qAF after surgery (estimate: 98.56, p = 0.006) and nuclear opacities showed a trend toward an increased change (estimate: 48.8, p = 0.095). Considerable interactions were identified between baseline qAF and cortical opacities, nuclear opacities and posterior subcapsular opacities, as well as nuclear opacities and cortical opacities (p = 0.012, p = 0.064 and p = 0.069, respectively). Quantitative autofluorescence signals are significantly reconstituted after cataract surgery and LOCS III gradings are well associated with post-surgical qAF values. Careful consideration of age-related lens opacities is vital for the correct interpretation of qAF, especially in retinal diseases affecting the elderly.ClinicalTrials.gov Identifier: NCT03465124.
Cancer-specific immune evasion and substantial heterogeneity within cancer types provide evidence for personalized immunotherapy
Authors: Thelen Martin , Wennhold Kerstin , Lehmann Jonas , Garcia-Marquez Maria , Klein Sebastian , Kochen Elena , Lohneis Philipp , Lechner Axel , Wagener-Ryczek Svenja , Plum Patrick Sven , Velazquez Camacho Oscar , Pfister David , Dörr Fabian , Heldwein Matthias , Hekmat Khosro , Beutner Dirk , Klussmann Jens Peter , Thangarajah Fabinshy , Ratiu Dominik , Malter Wolfram , Merkelbach-Bruse Sabine , Bruns Christiane Josephine , Quaas Alexander , von Bergwelt-Baildon Michael , Schlößer Hans A. 
Published: June 2021

AbstractThe immune response against cancer is orchestrated by various parameters and site-dependent specificities have been poorly investigated. In our analyses of ten different cancer types, we describe elevated infiltration by regulatory T cells as the most common feature, while other lymphocyte subsets and also expression of immune-regulatory molecules on tumor-infiltrating lymphocytes showed site-specific variation. Multiparametric analyses of these data identified similarities of renal and liver or lung with head and neck cancer. Co-expression of immune-inhibitory ligands on tumor cells was most frequent in colorectal, lung and ovarian cancer. Genes related to antigen presentation were frequently dysregulated in liver and lung cancer. Expression of co-inhibitory molecules on tumor-infiltrating T cells accumulated in advanced stages while T-cell abundance was related to enhanced expression of genes related to antigen presentation. Our results promote evaluation of cancer-specific or even personalized immunotherapeutic combinations to overcome primary or secondary resistance as major limitation of immune-checkpoint inhibition.
Increased susceptibility to dextran sulfate-induced mucositis of iron-overload β-thalassemia mice, another endogenous cause of septicemia in thalassemia
Authors: Visitchanakun Peerapat , Panpetch Wimonrat , Saisorn Wilasinee , Chatthanathon Piraya , Wannigama Dhammika Leshan , Thim-uam Arthid , Svasti Saovaros , Fucharoen Suthat , Somboonna Naraporn , Leelahavanichkul Asada 
Published: June 2021

Abstract
Enterocyte damage and gut dysbiosis are caused by iron-overload in thalassemia (Thl), possibly making the gut vulnerable to additional injury. Hence, iron-overload in the heterozygous β-globin deficient (Hbbth3/+) mice were tested with 3% dextran sulfate solution (DSS).
With 4 months of iron-gavage, iron accumulation, gut-leakage (fluorescein isothiocyanate dextran (FITC-dextran), endotoxemia, and tight junction injury) in Thl mice were more prominent than WT mice. Additionally, DSS-induced mucositis in iron-overloaded mice from Thl group was also more severe than the WT group as indicated by mortality, liver enzyme, colon injury (histology and tissue cytokines), serum cytokines, and gut-leakage (FITC-dextran, endotoxemia, bacteremia, and the detection of Green-Fluorescent Producing Escherichia coli in the internal organs after an oral administration). However, Lactobacillus rhamnosus GG attenuated the disease severity of DSS in iron-overloaded Thl mice as indicated by mortality, cytokines (colon tissue and serum), gut-leakage (FITC-dextran, endotoxemia, and bacteremia) and fecal dysbiosis (microbiome analysis). Likewise, Lactobacillus conditioned media (LCM) decreased inflammation (supernatant IL-8 and cell expression of TLR-4, nuclear factor κB (NFκB), and cyclooxygenase-2 (COX-2)) and increased transepithelial electrical resistance (TEER) in enterocytes (Caco-2 cells) stimulated by lipopolysaccharide (LPS) and LPS plus ferric ion.
In conclusion, in the case of iron-overloaded Thl, there was a pre-existing intestinal injury that wask more vulnerable to DSS-induced bacteremia (gut translocation). Hence, the prevention of gut-derived bacteremia and the monitoring on gut-leakage might be beneficial in patients with thalassemia.