bluuebunny/crossref_metadata_2025_split huggingface dataset:
Does the Radius Contain Bone Marrow Mesenchymal Stem Cells?-A Comparison between Cells of the Iliac Crest and Radius in Kienböck’s Disease
Authors: Ogawa Takeshi , Sugaya Hisashi , Hara Yuki , Yoshii Yuichi , Ochiai Naoyuki , Yamazaki Masashi 
Published: May 2021

Background: Numerous studies have indicated the presence of mesenchymal stem cells (MSCs) in the bone marrow aspirated from the vertebral body, distal femur, proximal tibia, humeral head, sternum, and iliac crest. However, their presence has not been reported in the radius thus far. In this study, we aimed to compare the number of MSCs in bone marrow aspirated from radius and iliac crest in patients with Kienböck’s disease. Furthermore, we examined the association between the number of MSCs in the bone marrow and patient age. Methods: A total of 17 patients were recruited. Owing to difficulties in obtaining samples for 5 cases, only 12 cases were included. Hematological analyses and fibroblastic colony-forming unit (CFU-F) assay were performed using bone marrow samples aspirated from the radius (group R), the first sample aspirated from the iliac crest (group I-1), and the second sample aspirated from the iliac crest (group I-2). The CFU-F numbers among the three groups were compared using Mann–Whitney U-test. Pearson’s correlation coefficient was calculated to evaluate the association between the CFU-F numbers and patient age. Results: The average numbers of CFU-Fs/ml in the bone marrow samples from the R, I-1, and I-2 groups were 3.4, 57.3, and 13.7, respectively. The CFU-F number in Group I-1 was significantly higher than that in the other two groups; the CFU-F number was lower in group R than in group I-2. The correlation coefficients were -0.168, 0.166, and 0.036 for samples from groups R, I-1, and I-2, respectively. No significant association between the CFU-F numbers and patient age was observed. Conclusions: The presence of MSCs in the radius was indicated by CFU-Fs in patients with Kienböck’s disease. The number of CFU-Fs was lower in the radius than in the iliac crest; the CFU-F number was not associated with patient age.
Long-Term Evaluation of Visual Outcomes and Patient Satisfaction after Binocular Implantation of a Bioanalogic Lens
Authors: Wagner Sylwia , Wagner Grzegorz , Mrukwa-Kominek Ewa 
Published: May 2021

Purpose. Long-term evaluation of the visual refractive outcomes and the quality of life after implantation of the WIOL-CF (Medicem, Czech Republic) in both eyes. Design. retrospective, nonrandomized noncomparative case series. Methods. 50 eyes of 25 patients, including 11 women (44%) and 14 men (56%). The age range of the patients was 38 to 77 years (mean age 55.48 ± 10.97 years). All patients underwent bilateral implantation of the WIOL-CF. Exclusion criteria were previous ocular surgeries except for cataract surgery and refractive lens exchange, irregular corneal astigmatism of >1.0 diopter, and ocular pathologies or corneal abnormalities. Postoperative examinations were performed at 14 days and 3, 6, 12 months of surgery; the last follow-up was between 24 and 36 months after the procedure. All exams included manifest refraction, monocular uncorrected visual acuity (UCVA) and distance-corrected visual acuity (DCVA) in 5 m (Snellen), monocular uncorrected visual acuity in 70 cm and 40 cm (Jeager) and binocular UCVA, DCVA in 5 m, 70 cm, and 40 cm, binocular contrast sensitivity (CS) under photopic conditions, binocular defocus curves, high-order aberrations, quality-of-vision VF-14 questionnaire, and spectacle independence. Results. Significant improvement in monocular visual acuity at all distances was demonstrated; the mean postoperative spherical equivalent was 0.32 ± 0.45D. The postoperative means of binocular distance UCVA and BCVA were also improved (

p

< .001) and so were the mean uncorrected intermediate VA (2.053 ± 1.268) and near uncorrected VA (2.737 ± 1.447). There was a significant improvement in contrast sensitivity at all spatial frequencies and higher-order aberration, compared to preoperative results. Conclusions. The evaluation of a WIOL-CF showed good distance, intermediate, and near visual acuity. Contrast sensitivity increased after surgery in all spatial frequencies. Patient satisfaction was high despite some optical phenomena. The rate of postoperative spectacle independence also turned out high. Financial Disclosure. No author has a financial or proprietary interest in any material or method mentioned.
Clinical Outcomes in Patients with Multi-Hit TP53 Chronic Lymphocytic Leukemia Treated with Ibrutinib
Authors: Brieghel Christian , Aarup Kathrine , Torp Mathias H. , Andersen Michael A. , Yde Christina W. , Tian Xin , Wiestner Adrian , Ahn Inhye E. , Niemann Carsten U. 
Published: May 2021

Abstract

Purpose:
TP53 aberration (TP53 mutation and/or 17p deletion) is the most important predictive marker in chronic lymphocytic leukemia (CLL). Although each TP53 aberration is considered an equal prognosticator, the prognostic value of carrying isolated (single-hit) or multiple (multi-hit) TP53 aberrations remains unclear, particularly in the context of targeted agents.

Patients and Methods:
We performed deep sequencing of TP53 using baseline samples collected from 51 TP53 aberrant patients treated with ibrutinib in a phase II study (NCT01500733).

Results:
We identified TP53 mutations in 43 patients (84%) and del(17p) in 47 (92%); 9 and 42 patients carried single-hit and multi-hit TP53, respectively. The multi-hit TP53 subgroup was enriched with younger patients who had prior treatments and unmutated immunoglobulin heavy-chain variable region gene status. We observed significantly shorter overall survival, progression-free survival (PFS), and time-to-progression (TTP) in patients with multi-hit TP53 compared with those with single-hit TP53. Clinical outcomes were similar in patient subgroups stratified by 2 or &gt;2 TP53 aberrations. In multivariable analyses, multi-hit TP53 CLL was independently associated with inferior PFS and TTP. In sensitivity analyses, excluding mutations below 1% VAF demonstrated similar outcome. Results were validated in an independent population-based cohort of 112 patients with CLL treated with ibrutinib.

Conclusions:
In this study, single-hit TP53 defines a distinct subgroup of patients with an excellent long-term response to single-agent ibrutinib, whereas multi-hit TP53 is independently associated with shorter PFS. These results warrant further investigations on prognostication and management of multi-hit TP53 CLL.
See related commentary by Bomben et al., p. 4462
Objet trouvé: etnografia de rua – Paris
Authors: Tacca Fernando de 
Published: May 2021

Esse pequeno ensaio faz parte de uma proposta mais ampla sobre a cidade de Paris, e teve seu início em 2012, inspirado pela relação entre o livro “Pasages”, de Walter Benjamin e as fotografias de Eugéne Atget. Naquele momento, durante o período de um mês, foram produzidas imagens de ruas, vitrines, passagens, e de pequenos cantos, através de três tipos de câmeras, sendo uma delas analógica. Baseadas e inspiradas nos olhares técnicos de Atget, mas também produzidas e trabalhadas a partir de uma câmera cega (GoPRO) e de uma câmera Lomo olho de peixe, foram criadas sobreposições de imagens no próprio negativo para que se evidenciasse a decomposição de espaços monumentais através das imagens.
Como continuação da proposta, em um período mais amplo, entre março de 2016 e fevereiro de 2017, a partir do deambular e do flanar fotográfico pelas ruas de Paris, situações fortuitas e encontros casuais com objetos imprevistos, indicativa dos diálogos que propõe a cidade, retomaram o pensamento de Jean Baudrillard, e fizeram latente e constante a busca por uma existência ou uma conversa entre os objetos e seu entorno. Objetos banais, esquecidos, descartados ou interferidos, que ao olhar fotográfico criam pulsões, transformando o fotógrafo em um coletor, em seu sentido mais antropológico.
Temporal Incidence and Predictors of High‐Grade Atrioventricular Block After Transcatheter Aortic Valve Replacement
Authors: El‐Sabawi Bassim , Welle Garrett A. , Cha Yong‐Mei , Espinosa Raúl E. , Gulati Rajiv , Sandhu Gurpreet S. , Greason Kevin L. , Crestanello Juan A. , Friedman Paul A. , Munger Thomas M. , Rihal Charanjit S. , Eleid Mackram F. 
Published: May 2021

Background
The temporal incidence of high‐grade atrioventricular block (HAVB) after transcatheter aortic valve replacement (TAVR) is uncertain. As a result, periprocedural monitoring and pacing strategies remain controversial. This study aimed to describe the temporal incidence of initial episode of HAVB stratified by pre‐ and post‐TAVR conduction and identify predictors of delayed events.

Methods and Results

Consecutive patients undergoing TAVR at a single center between February 2012 and June 2019 were retrospectively assessed for HAVB within 30 days. Patients with prior aortic valve replacement, permanent pacemaker (PPM), or conversion to surgical replacement were excluded. Multivariable logistic regression was performed to assess predictors of delayed HAVB (initial event >24 hours post‐TAVR). A total of 953 patients were included in this study. HAVB occurred in 153 (16.1%). After exclusion of those with prophylactic PPM placed post‐TAVR, the incidence of delayed HAVB was 33/882 (3.7%). Variables independently associated with delayed HAVB included baseline first‐degree atrioventricular block or right bundle‐branch block, self‐expanding valve, and new left bundle‐branch block. Forty patients had intraprocedural transient HAVB, including 16 who developed HAVB recurrence and 6 who had PPM implantation without recurrence. PPM was placed for HAVB in 130 (13.6%) (self‐expanding valve, 23.7% versus balloon‐expandable valve, 11.9%;
P
<0.001). Eight (0.8%) patients died by 30 days, including 1 unexplained without PPM present.

Conclusions
Delayed HAVB occurs with higher frequency in patients with baseline first‐degree atrioventricular block or right bundle‐branch block, new left bundle‐branch block, and self‐expanding valve. These findings provide insight into optimal monitoring and pacing strategies based on periprocedural ECG findings.
50 Grades of Shade
Authors: Middel Ariane , AlKhaled Saud , Schneider Florian A. , Hagen Bjoern , Coseo Paul 
Published: May 2021

AbstractCities increasingly recognize the importance of shade to reduce heat stress and adopt urban forestry plans with ambitious canopy goals. Yet, the implementation of tree and shade plans often faces maintenance, water use, and infrastructure challenges. Understanding the performance of natural and nonnatural shade is critical to support active shade management in the built environment. We conducted hourly transects in Tempe, Arizona, with the mobile human-biometeorological station MaRTy on hot summer days to quantify the efficacy of various shade types. We sampled sun-exposed reference locations and shade types grouped by urban form, lightweight/engineered shade, and tree species over multiple ground surfaces. We investigated shade performance during the day, at peak incoming solar, at peak air temperature, and after sunset using three thermal metrics: the difference between a shaded and sun-exposed location in air temperature (∆Ta), surface temperature (∆Ts), and mean radiant temperature (∆TMRT). Air temperature did not vary significantly between shade groups, but ∆TMRT spanned a 50°C range across observations. At daytime, shade from urban form most effectively reduced Ts and TMRT, followed by trees and lightweight structures. Shade from urban form performed differently with changing orientation. Tree shade performance varied widely; native and palm trees were least effective, while nonnative trees were most effective. All shade types exhibited heat retention (positive ∆TMRT) after sunset. Based on the observations, we developed characteristic shade performance curves that will inform the City of Tempe’s design guidelines toward using “the right shade in the right place” and form the basis for the development of microclimate zones (MCSz).
The Influence of Ground-Relative Flow and Friction on Near-Surface Storm-Relative Helicity
Authors: Flournoy Matthew D. , Rasmussen Erik N. 
Published: May 2021

AbstractRecent studies have highlighted the importance of near-ground storm-relative helicity (SRH) in supercell and tornado processes and how surface friction can play a role. In this study, we use an analytical approach to examine how uniform changes to the ground-relative wind profile above the near-ground layer influence SRH within the near-ground layer. We show how the ground-relative influence of surface friction alters the near-ground shear profile. For idealized semicircular and straight shear profiles, increasing preexisting ground-relative flow above the near-ground layer yields increasing SRH. The magnitude of the SRH increase is sensitive to storm motion, with more deviant motion yielding greater SRH increases given the same increase in ground-relative flow. Supercells may be more susceptible to storm-induced SRH enhancements given their deviant motion and ability to increase ground-relative flow in the background environment.
Impact of Reduced Arctic Sea Ice on Northern Hemisphere Climate and Weather in Autumn and Winter
Authors: Chripko Svenya , Msadek Rym , Sanchez-Gomez Emilia , Terray Laurent , Bessières Laurent , Moine Marie-Pierre 
Published: May 2021

AbstractThe Northern Hemisphere transient atmospheric response to Arctic sea decline is investigated in autumn and winter, using sensitivity experiments performed with the CNRMCM6-1 high-top climate model. Arctic sea ice albedo is reduced to the ocean value, yielding ice-free conditions during summer and a more moderate sea ice reduction during the following months. A strong ampli_cation of temperatures over the Arctic is induced by sea ice loss, with values reaching up to 25°C near the surface in autumn. Signi_cant surface temperature anomalies are also found over the mid-latitudes, with a warming reaching 1°C over North America and Europe, and a cooling reaching 1°C over central Asia. Using a dynamical adjustment method based on a regional reconstruction of circulation analogs, we show that the warming over North America and Europe can be explained both by changes in the atmospheric circulation and by the advection of warmer oceanic air by the climatological ow. In contrast, we demonstrate that the sea-ice induced cooling over central Asia is solely due to dynamical changes, involving an intensi_cation of the Siberian High and a cyclonic anomaly over the Sea of Okhotsk. In the troposphere, the abrupt Arctic sea ice decline favours a narrowing of the subtropical jet stream and a slight weakening of the lower part of the polar vortex that is explained by a weak enhancement of upward wave activity toward the stratosphere. We further show that reduced Arctic sea ice in our experiments is mainly associated with less severe cold extremes in the mid-latitudes.
Simulating observed cloud transitions in the northeast Pacific during CSET
Authors: Blossey Peter N. , Bretherton Christopher S. , Mohrmann Johannes 
Published: May 2021

AbstractThe goal of this study is to challenge a large eddy simulation model with a range of observations from a modern field campaign and to develop case studies useful to other modelers. The 2015 Cloud System Evolution in the Trades (CSET) field campaign provided a wealth of in situ and remote sensing observations of subtropical cloud transitions in the summertime Northeast Pacific. Two Lagrangian case studies based on these observations are used to validate the thermodynamic, radiative and microphysical properties of large eddy simulations (LES) of the stratocumulus to cumulus transition. The two cases contrast a relatively fast cloud transition in a clean, initially well-mixed boundary layer vs. a slower transition in an initially decoupled boundary layer with higher aerosol concentrations and stronger mean subsidence. For each case, simulations of two neighboring trajectories sample mesoscale variability and the coherence of the transition in adjacent air masses. In both cases, LES broadly reproduce satellite and aircraft observations of the transition. Simulations of the first case match observations more closely than for the second case, where simulations underestimate cloud cover early in the simulations and overestimate cloud top height later. For the first case, simulated cloud fraction and liquid water path increase if a larger cloud droplet number concentration is prescribed. In the second case, precipitation onset and inversion cloud breakup occurs earlier when the LES domain is chosen large enough to support strong mesoscale organization.
Characteristics of Long-Duration Heavy Precipitation Events along the West Coast of the United States
Authors: Moore Benjamin J. , White Allen B. , Gottas Daniel J. 
Published: May 2021

AbstractProlonged periods (e.g., several days or more) of heavy precipitation can result in sustained high-impact flooding. Herein, an investigation of long-duration heavy precipitation events (HPEs), defined as periods comprising ≥3 days with precipitation exceeding the climatological 95th percentile, is conducted for 1979–2019 for the U.S. West Coast, specifically Northern California. An objective flow-based categorization method is applied to identify principal large-scale flow patterns for the events. Four categories are identified and examined through composite analyses and case studies. Two of the categories are characterized by a strong zonal jet stream over the eastern North Pacific, while the other two are characterized by atmospheric blocking over the central North Pacific and the Bering Sea–Alaska region, respectively. The composites and case studies demonstrate that the flow patterns for the HPEs tend to remain in place for several days, maintaining strong baroclinicity and promoting occurrences of multiple cyclones in rapid succession near the West Coast. The successive cyclones result in persistent water vapor flux and forcing for ascent over Northern California, sustaining heavy precipitation. For the zonal jet patterns, cyclones affecting the West Coast tend to occur in the poleward jet exit region in association with cyclonic Rossby wave breaking. For the blocking patterns, cyclones tend to occur in association with anticyclonic Rossby wave breaking on the downstream flank of the block. For Bering Sea–Alaska blocking cases, cyclones can move into this region in conjunction with cyclonically breaking waves that extend into the eastern North Pacific from the upstream flank of the block.
The cytotoxic conjugate of highly internalizing tetravalent antibody for targeting FGFR1-overproducing cancer cells
Authors: Poźniak Marta , Porębska Natalia , Krzyścik Mateusz Adam , Sokołowska-Wędzina Aleksandra , Jastrzębski Kamil , Sochacka Martyna , Szymczyk Jakub , Zakrzewska Małgorzata , Otlewski Jacek , Opaliński Łukasz 
Published: May 2021

Abstract
Background
Antibody drug conjugates (ADCs) represent one of the most promising approaches in the current immuno-oncology research. The precise delivery of cytotoxic drugs to the cancer cells using ADCs specific for tumor-associated antigens enables sparing the healthy cells and thereby reduces unwanted side effects. Overexpression of fibroblast growth factor receptor 1 (FGFR1) has been demonstrated in numerous tumors and thereby constitutes a convenient molecular target for selective cancer treatment. We have recently engineered tetravalent anti-FGFR1 antibody, T-Fc, and have demonstrated that it displays extremely efficient internalization into FGFR1 producing cells, a feature highly desirable in the ADC approach. We have revealed that T-Fc mediates clustering of FGFR1, largely enhancing the uptake of FGFR1-T-Fc complexes by induction of clathrin-independent endocytic routes. The aim of this study was to obtain highly internalizing cytotoxic conjugate of the T-Fc for specific delivery of drugs into FGFR1-positive cancer cells.

Methods
Conjugation of the T-Fc to a cytotoxic payload, vcMMAE, was carried out via maleimide chemistry, yielding the T-Fc-vcMMAE. The specific binding of the T-Fc-vcMMAE conjugate to FGFR1 was confirmed in vitro with BLI technique. Confocal microscopy and flow cytometry were applied to determine FGFR1-dependence of the T-Fc-vcMMAE internalization. Western blot analyses of FGFR1-dependent signaling were conducted to assess the impact of the T-Fc-vcMMAE on FGFR1 activation and initiation of downstream signaling cascades. Finally, using FGFR1-negative and FGFR1-possitive cell lines, the cytotoxic potential of the T-Fc-vcMMAE was evaluated.

Results
We have performed the efficient conjugation of the tetravalent engineered antibody with a cytotoxic drug and generated FGFR1-specific ADC molecule, T-Fc-vcMMAE. We have demonstrated that T-Fc-vcMMAE conjugate exhibits high selectivity and affinity for FGFR1, similarly to T-Fc. Furthermore, we have shown that T-Fc constitutes an effective drug delivery vehicle as T-Fc-vcMMAE was efficiently and selectively internalized by FGFR1-producing cells leading to their death. Interestingly, we show that the efficiency of the uptake of T-Fc-vcMMAE corresponds well with the cytotoxicity of the conjugate, but doesn’t correlate with the FGFR1expression level.

Conclusion
Our results show that T-Fc-vcMMAE fulfills the key criteria for the successful cytotoxic drug carrier in a targeted approach against FGFR1-positive cancer cells. Furthermore, our data implicate that not solely expression level of the receptor, but rather its cellular trafficking should be taken into account for selection of suitable molecular targets and cancer models for successful ADC approach.
A spatial analysis of the epidemiology of HIV-infected students in Zhejiang province, China
Authors: Chen Wanjun , Yang Jiezhe , Jiang Jun , He Lin , Xu Yun , Zheng Jinlei , Jiang Jianmin , Pan Xiaohong 
Published: May 2021

Abstract
Background
The upsurge in HIV infections among students is a matter of particular concern. However, few studies have explored the epidemiological characteristics including the risky sexual networking of HIV-infected students in Zhejiang province, China.

Methods
Using the provincial surveillance data of HIV-infected students, we conducted a retrospective epidemiology study to describe the epidemiological characteristics of 628 newly diagnosed cases from 2011 to 2016 and detailed information of 124 cases from 2015 to 2016. Spatial analyses were conducted using ArcGIS software, and statistical analyses were performed using SPSS software.

Results
A total of 628 cases of HIV/AIDS were diagnosed among students in Zhejiang Province, China between 2011 and 2016. The cases showed an overall increasing trend over time, while the proportions of students with HIV disease status, cases diagnosed by HIV voluntary counseling and testing (VCT), and cases of homosexual transmission remained stable over time. Significant spatial heterogeneity in the cases was seen at the county level. Detailed data on 124 HIV-positive individuals collected from the local Center for Disease Control and Prevention (CDC) from 2015 and 2016, showed that the majority of them (85.5%,) engaged in homosexual behavior, and 93.4% had sex with casual partners. These partners included not only social members, but also other students. Online dating applications represented the most common means of seeking and communicating with homosexual partners. The level of awareness regarding the risk of HIV infection, and the amount coverage of face-to-face education towards students were both low.

Conclusions
HIV infections among students were characterized by increasing trend and spatial clustering in Zhejiang Province between 2011 and 2016, with homosexual sexual activity being the main mode of infection. Interventions are urgently required to prevent HIV infection in this population by increasing awareness of the disease. HIV testing programs and information regarding disease prevention specifically through online dating applications are needed.