bluuebunny/crossref_metadata_2025_split huggingface dataset:
Application of Electrical Impedance Measurements’ Method for Studies of Bubble Flows
Authors: Konovalov I. A. , Chesnokov A. A. , Barinov A. A. , Dmitriev S. M. , Khrobostov A. E. , Makarov M. A. 
Published: October 2021

One of the important tasks in carrying out a computational justification of the reliability and safety of equipment that is part of the projected nuclear power plants today is the modeling of the bubbly regime of the coolant flow. In this regard the aim of this work is the use of extended methods of using matrix conductometric systems which are widespread in research practice for study of gas-liquid flows.The work uses a method of primary processing of experimental data aimed at eliminating of excess conductivity in the cells of the developed wire mesh sensor which makes it possible to obtain the values of the true volumetric gas content in the investigated area.Subsequent analysis of the possibilities to estimate the volumes of registered gas bubbles by the gradient method as well as the size of the interface in the sensor cells which plays a key role in modeling the interfacial heat and mass transfer.Comparison of readings values with the control instruments cues showed a good agreement. The presented work is an adaptation of the use of a conductometric measuring system for the study of multicomponent flows with the aim of further application for the study of two-component flows in the channels of the core simulator using wire mesh sensors.
Evaluation of the Magnet Breakaway Force Measurement Accuracy of the NT-800 Sensors for Early Detection of Defects of Their Manufacturing
Authors: Kutsepau A. Yu. , Kren A. P. , Hnutsenka Y. V. 
Published: October 2021

Сontrol of mechanical stresses formed with the deposition of nickel coatings plays an important role in the diagnosis of coatings’ technical condition. Large internal stresses can lead to cracking or flaking of coatings which is completely unacceptable for critical parts and assembly units used, for example, in space technology for which reliability is of paramount importance. An important aspect of internal stresses monitoring is the measurement error of the instruments used. The purpose of this work was to determine the characteristics of the device sensors, which make the assessment of their manufacturing possible at the preliminary stage of the measuring equipment assembling in order to maintain the required accuracy of subsequent measurements.In most cases the measurement error assessment is possible only after the equipment manufacture and calibration. In this paper it is proposed to evaluate the accuracy characteristics of device sensors based on the precision (repeatability and reproducibility) of the primary informative parameter recording. In the case of the NT-800 device that was developed at the Institute of Applied Physics of the National Academy of Sciences of Belarus the effect of precision characteristics deterioration on the eventual measurement error is demonstrated. Determining the precision parameters before establishing correlation dependences between the primary informative parameter and the measured characteristic is proposed in order to reject poorly manufactured sensors and reduce labor costs.In particular, measurements of the magnitude proportional to the magnetic breakaway force were carried out using the NT-800 device with nickel specimens simulating coatings with a thickness of 200 to 700 μm and a rolling value from 0 to 40 %. It was established that in the case of well-made sensors the variation coefficient calculated from the dispersion of repeatability is in the range 0.2–0.6 %, and the variation coefficient calculated from the dispersion of reproducibility does not exceed 0.9 %. In the case of a sensor with the sensitive element parameters worsened, the variation coefficient of repeatability and reproducibility were up by one and a half times. Deterioration of the precision characteristics resulted in significant changes in the readings of the calibrated instrument. Thus the absolute measurement error for a sensor with a poorly made sensitive element turned out to be approximately 3 times higher in the range of 200– 300 MPa than that for a sensor with good precision parameters.
Calculation of the Effective Energy Release Centerʼs Position of Inorganic Scintillation Detectors for Calibration at Small “Source–Detector” Distances
Authors: Lukashevich R. V. , Fokov G. A. 
Published: October 2021

Inorganic scintillation detectors are widely used to measure of dose rate in the environment due to their high sensitivity to photon radiation. A distinctive feature when using such detectors is the need to take into account of the position of the effective energy release center. This peculiarity is actual when using measuring instruments with inorganic scintillation detectors as working standards during calibration at short “source–detector” distances in conditions of low-background shield or using a facility with protection from external gamma radiation background in the dose rate range from 0.03 to 0.3 μSv/h (μGy/h). The purpose of this work was to calculate the position of the effective energy release center of NaI(Tl) scintillation detectors and to take it into account when working at short “source–detector” distances.An original method of determining the position of the effective energy release center when irradiating the side and end surfaces of inorganic scintillation detector with parallel gamma radiation flux and point gamma radiation sources at small “source–detector” distances using Monte Carlo methods is proposed. The results of calculations of the position of the effective energy release center of NaI(Tl) based detectors of “popular” sizes for the cases of parallel gamma radiation flux and point sources of gamma radiation at small “source–detector” distances are presented. The functional dependences of the position of the effective energy release center of NaI(Tl) based detectors on the distance to the point gamma radiation sources and the energy of gamma radiation sources are presented.As a result of the study it was found that for scintillation NaI(Tl) detectors of medium size (for example, Ø25×40 mm or Ø40×40 mm) the point gamma radiation source located at a distance of 1 m or more, creates a radiation field which does not differ in characteristics from the radiation field created by a parallel flux of gamma radiation. It is shown that approaching the point gamma radiation source to the surface of scintillation detector leads to displacement of the position of the effective energy release center to the surface of the detector.
Development and Successful Field Trial of Retrievable, Instrumented & Tandem Downhole Isolation Valve RIT-DIV System
Authors: Amangeldiyeva Diana , Aliyeva Aida Askarovna , Amanbayev Yerlan , Toralde Julmar Shaun Sadicon , Higginson Timothy Peter , Tilekkabyl Akzharkyn Assetkyzy , Nurseiitov Dinmukhamed Jangaliuly , Fan Yong , Cameron Gareth 
Published: October 2021

Abstract
This paper describes the development and field deployment of a new downhole isolation valve system called the Retrievable, Instrumented & Tandem Downhole Deployment Valve (RIT-DDV). The purpose of this technology is to provide a temporary mechanical barrier to isolate and monitor the well during drilling operations in an environment where a full column of single-phase fluid cannot be maintained.
The RIT-DDV is based on predominantly used downhole isolation valve (DIV) design and technology, which is a hydraulic flapper-type isolation device installed in the casing that seals the open hole during pipe tripping operations.
The key features of the new RIT-DDV systems are dual flapper valves with three downhole pressure and temperature gauges to take measurements above, between, and below the flappers. The advantage of this configuration is that it enhances safety by enabling double-block-and-bleed system functionality, providing valve redundancy, and moreover allowing for continuous real-time monitoring of downhole well conditions. In addition, the RIT-DDV is designed to be reusable and can be tested upon installation and replaced if necessary. The RIT-DDV system enabled the operator to isolate and monitor the well while drilling through a depleted formation that prevented drilling with a full column of single-phase drilling fluid. The RIT-DDV was successfully trialed in western Kazakhstan and demonstrated the potential of this technology to enhance the safety of drilling heavily fractured carbonate formations with reservoir fluids containing hydrogen sulfide (H2S) / carbon dioxide (CO2) that are prone to total loss of circulation. The downhole pressure / temperature monitoring capabilities that the system provides within the casing string helped drill through the depleted fractured carbonate reservoir section without incurring non-productive time (NPT).
Thymol improves salinity tolerance of tobacco by increasing the Na+ efflux and enhancing the content of NO and GSH
Authors: xu liang , Song Jia-Qian , wang yuelin , Liu Xiao-Han , Li Xue-Li , Zhang Bo , Li Ai-Jie , Ye Xie-Feng , Wang Jing , Wang Peng 
Published: October 2021

Abstract
Plants have evolved a lot of strategies to improve salt tolerance to cope with salt stress. Recent studies have suggested that thymol (a nature medicine) enhances the plant tolerance against abiotic stresses, but the mechanisms are rarely known. Here, we found that thymol played an important role in maintaining root growth under salt stress. Thymol rescued root growth from salt stress via ameliorating ROS (reactive oxygen species) accumulation, lipid peroxidation, and cell death. In addition, thymol enhanced the level of NO (nitric oxide) and GSH (glutathione) to repress ROS accumulation, further protecting the stability of cell membrane. Thymol-induced Na+ efflux in roots and leaves under salt stress may depend on the upregulation of SOS1, HKT1 and NHX1. Consequently, all of these evidences suggested that thymol improved tobacco salt tolerance via enhancing NO and GSH content as well as inducing Na+ efflux.
The Tobacco Metabolite NNK Enhances Pancreatic Cancer Cell Stemness and Chemoresistance by Activating the β2AR-Akt Autophagy Axis
Authors: Chen Xin , Zhang Weifan , Liu Rujuan , Zhu Zeen , Gong Mengyuan , Wang Qiqi , Qian Weikun , Wu Zheng , Ma Qingyong , Wang Zheng 
Published: October 2021

Abstract
Background: Low responsiveness to chemotherapy is an important cause of poor prognosis in pancreatic cancer. Smoking is a high-risk factor for pancreatic cancer and its resistance to gemcitabine; however, the underlying mechanisms remain unclear. 4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) is the main metabolite of tobacco burning and has been shown to be associated with cancer development and chemoresistance, but in pancreatic cancer, its mechanism remains poorly understood.Methods: The effect of NNK on pancreatic cancer cell viability was confirmed by using Cell Counting Kit-8 and colony formation assays. Stem cell sphere formation assays and western blotting/qPCR measurements of stemness-related molecules were used to detect pancreatic cancer cell stemness. The pancreatic cancer autophagy status was evaluated by immunofluorescence staining of LC3 and western blotting/qPCR analysis of autophagy-related molecules.Results: NNK promoted stemness and gemcitabine resistance in pancreatic cancer cell lines. Furthermore, NNK intervention increased autophagy and changed the expression levels of autophagy-related markers, which preliminarily confirmed the activation of autophagy by NNK. Finally, the results showed that NNK-promoted stemness, and gemcitabine resistance was activated by the autophagy pathway, which was mediated by the β2AR-Akt signalling pathway.Conclusions: Autophagy induced by activating the NNK-induced β2AR-Akt signalling pathway promoted stemness and gemcitabine resistance in pancreatic cancer cells.
Insights Into Vacuum Preloading Consolidation of Landfill Sludge Based on Fe2+-activated Sodium Persulfate
Authors: Wu Yajun , Zhang Xingtao , Zhang Xudong , Zhang haiqiang , Wang Yaoyi , Ye Peng 
Published: October 2021

Abstract
To further reduce the in-situ sludge from landfill, Fe2+-activated sodium persulfate combined with vacuum preloading was first proposed. Firstly, the effects of optimal Na2S2O8 dosage on the landfill sludge (LS) were investigated by the vacuum filtration experiments. Then, vacuum preloading experiments were conducted on the sludge with different Na2S2O8 dosage to study the water content, water discharge and settlement. Besides, sludge particle size diversification was carried out by particle size distribution and scanning electron microscopy (SEM) tests. The results were summarized as follows: the specific resistance of filtration (SRF) of LS could be reduced by 98.5% mostly when the Na2S2O8 dosage was 30%; the particle size became significantly smaller, and large particles were converted to small particles; the water content dropped from 86.9% to 58.3%; the SEM test manifested the oxidation of sodium persulfate caused the destruction of the glial structure of the sludge and the recombination of partial particles.
Polymorphic crystalline wetting layers on crystal surfaces
Authors: Wang Xipeng , Li Bo , Li Mengmeng , Han Yilong 
Published: October 2021

Abstract
Analogous to surface premelting, we propose that a crystal surface can undergo a pre-solid-solid transition, i.e. developing a thin polymorphic crystalline layer before reaching the solid-solid transition temperature if two crystals can form a low-energy coherent interface. We confirm this in simulations and colloid experiments at single-particle resolution. The power-law increase of surface layer thickness is analogous to premelting. Different kinetics and reversibilities of surface-crystal growth are observed in various systems. Surface crystals exist not only under thermal equilibrium, but also during melting, crystallization, and grain coarsening. Furthermore, the premelting and pre-solid-solid transition can coexist, resulting double surface wetting layers. We hypothesize that such surface phenomena exist in atomic and molecular crystals, which provide a novel way to tune material properties.
Extrapolating potential crop damage by insect pests based on land use data: examining inter-regional generality in agricultural landscapes
Authors: Tabuchi Ken , Takahashi Akihiko , Uesugi Ryuji , Okudera Shigeru , Yoshimura Hideto 
Published: October 2021

Abstract
ContextInter-regional relationships between landscape factors and biological responses under natural conditions are important but difficult to predict because of the differences in each landscape context and local environment. ObjectivesTo examine the inter-regional variability in relation to landscape factors and the biological response of an insect pest, we extrapolated a damage prediction model (‘the original model’) for rice using land-use data. We hypothesized that the original model would be applicable to new regions, but the predictive accuracy would be reduced. We predicted that adjusting the parameter coefficients would improve model performance (‘the adjusted model’). MethodsA field experiment was conducted in two regions that had a similar landscape context with the original region, in different years for each region, for four years in total. The proportion of rice damage and surrounding land-use within a 300-m radius was investigated. ResultsWhen ‘the original model’ was assigned to combined data from the original and extrapolated regions, the relationship between observed and predicted values was statistically significant, suggesting that there was an inter-regional common relationship. The relationship was not statistically significant if the model was applied only to the new regions. The adjusted model improved by 14% compared with the original model.ConclusionsThese results imply that in this pest–crop system, a common inter-regional biological response to arthropods is likely because of landscape factors, although local environmental factors must be considered. Application of such relationships is needed to identify or prevent pest hazards by offering region-wide management options.
Rapidly Mutating Y Chromosomal STRs in Deep-Rooted Endogamous Pedigrees
Authors: Nazir Shahid , Adnan Atif , Rehman Rahat , Jawad Muhammad , Almheiri Reem , Lootah Reem , Alreyami Afra , Almarri Iman , Rakha Allah , Hadi Sibte 
Published: October 2021

Abstract
Y-chromosome short tandem repeat polymorphisms (Y-STRs) are important in many areas of human genetics. Y chromosomal STRs being normally utilized in the field of forensic exhibit low haplotype diversity in endogamous populations and fail to discriminate among male relatives from same pedigree. Rapidly mutating Y-STRs (RM Y-STRs) have been paid much attention in last decade. These 13 RM Y STRs have high mutation rates (>10-2) and have considerably higher haplotype diversity and discrimination capacity than conventionally used Y-STRs showing remarkable power, when it comes to differentiation in paternal lineages in endogamous populations. Previously, we have analyzed 2–4 generation, 99 pedigrees covering 1568 pairs of men covering 1–6 meioses from all over Pakistan and 216 male relatives from 18 deep rooted endogamous Sindhi pedigrees covering 1-7 meioses. Here we are presenting 861 pairs of men from 63 endogamous pedigrees covering 1-6 meioses from Punjabi population of Punjab, Pakistan. Mutations were frequently observed at DYF399 and DYF403 while no mutation was observed at DYS526a/b. The rate of differentiation ranged from 29.70% (first meiosis) to 80.95% (fifth meiosis) while overall (1 to 6 meiosis) differentiation was 59.46%. Combining previously published data with newly generated data, an overall differentiation rate was 38.79% based on 5176 pairs of men related by 1–20 meioses, while Y-filer differentiation was 9.24% based on 3864 pairs. Using father-son pair data from the present and previous studies, we also provide updated RM Y-STR mutation rates.
A High-Throughput Skim-sequencing Approach for Genotyping, Dosage Estimation and Identifying Translocations
Authors: Adhikari Laxman , Shrestha Sandesh , Wu Shuanyge , Crain Jared , Gao Liangliang , Evers Byron , Wilson Duane , Ju Yoonha , Koo Dal-Hoe , Hucl Pierre , Pozniak Curtis , Walkowiak Sean , Wang Xiaoyun , Wu Jing , Glaubitz Jeffrey C. , DeHaan Lee , Friebe Bernd , Poland Jesse 
Published: October 2021

Abstract
The development of next generation sequencing (NGS) enabled a shift from array-based genotyping to high-throughput genotyping by directly sequencing genomic libraries. Even though whole genome sequencing was initially too costly for routine analysis in large populations, such as those utilized for breeding or genetic studies, continued advancements in genome sequencing and bioinformatics have provided the opportunity to utilize whole-genome information. As new sequencing platforms can routinely provide high-quality sequencing data for sufficient genome coverage, a limitation comes in the time and high cost of library construction when multiplexing a large number of samples. Here we describe a high-throughput whole-genome skim-sequencing (skim-seq) approach that can be utilized for a broad range of genotyping and genomic characterization. Using optimized low-volume Illumina Nextera chemistry, we developed a skim-seq method and combined up to 960 samples in one multiplex library using dual index barcoding. With the dual-index barcoding, the number of samples for multiplexing can be adjusted depending on amount of data required and extended to 3,072 samples or more. Panels of double haploid wheat lines (Triticum aestivum, CDC Stanley x CDC Landmark), wheat-barley (T. aestivum x Hordeum vulgare) and wheat-wheatgrass (Triticum durum x Thinopyrum intermedium) introgression lines as well as known monosomic wheat stocks were genotyped using the skim-seq approach. Bioinformatics pipelines were developed for various applications where sequencing coverage ranged from 1x down to 0.01x per sample. Using reference genomes, we detected chromosome dosage, identified aneuploidy, and karyotyped introgression lines from the low coverage skim-seq data. Leveraging the recent advancements in genome sequencing, skim-seq provides an effective and low-cost tool for routine genotyping and genetic analysis, which can track and identify introgressions and genomic regions of interest in genetics research and applied breeding programs.
Forecasting tropical ENSO-induced drought conditions using sea surface height in the Western Pacific
Authors: David Carlos Primo , Custado Manuel Justin , Sekhon Natasha , Ibarra Daniel 
Published: October 2021

Abstract
The interannual variability of rainfall caused by the El Niño Southern Oscillation (ENSO) results in significant changes in hydrologic conditions that affect entire countries’ water supply. Forecasting ENSO and its impacts are mainly based on Central Pacific Sea surface temperature (SST) anomalies which satisfactorily correlates with timing and, to a lesser extent, the intensity of drought conditions in the Philippines and the rest of the Western Pacific during the El Niño phase. Changes in sea surface height (SSH) are also brought upon by ENSO through density changes with temperature and oceanographic processes. Here, we report that the associative nature of SSH and drought as measured by surface runoff, has a stronger correlation (r > 0.693, p < 0.05) in terms of the expected timing, with 1 to 3 months lag time, and intensity compared to using traditional ENSO SST indices from the Central Pacific. Furthermore, since SSH is co-located with its corresponding forecasted decrease in runoff, a localized prediction can be made which further increases the accuracy of this predictive tool. In the wake of a changing climate, this work demonstrates the possibility of statistically forecasting the timing of precipitation, and thus the volume of surface water availability, using local SSH as an indicator. A tool which is necessary for time-sensitive management decision making in drought-prone tropical regions.
Fetal Gene Variants Associated With Birth Weight Protection in a Native High Altitude Ladakhi Population
Authors: Bhandari Sushil , Dolma Padma , Mukerji Mitali , Prasher Bhavana , Montgomery Hugh , Kular Dalvir , Jain Vandana , Dadhwal Vatsla , Williams David J , Bhattacharyaa Aniket , Gilbert Edmund , Cavalleri Gianpiero L , Hillman Sara L 
Published: October 2021

Abstract
Pathological low birth weight ‘fetal growth restriction’ (FGR) is an important predictor of adverse obstetric outcomes including stillbirth. It is more common amongst native lowlanders when gestating in the hypoxic environment of high altitude, whilst populations who have resided at high altitude for many generations are relatively protected. Genetic study of pregnant populations at high altitude allows for exploration of the hypoxic influence on FGR pathogenesis.Pregnant women were recruited from Sonam Norboo Memorial Hospital, Ladakh between February 2017- January 2019 in this study. Principal component, admixture and genome wide association analysis (GWAS) were applied on umbilical cord blood DNA samples from 316 neonates, to explore ancestry and the genetic influence on low birth weight.Our findings support Tibetan ancestry in the Ladakhi population, with subsequent admixture with neighboring Indo-Aryan populations. Fetal growth protection was evident in Ladakhi neonates. Seven loci from five different genomic regions (ZBTB38, ZFP36L2, HMGA2, CDKAL1, PLCG1) previously associated with birthweight, were likewise similarly associated here. In summary, the Ladakhi population show evidence of enrichment of variants in genes that may help mitigate altitude-associated fetal growth restriction, supporting novel biological pathways and therapeutic targets for FGR, worthy of further investigation.