bluuebunny/crossref_metadata_2025_split huggingface dataset:
The situation of echinococcosis in stray dogs in Turkey: the first finding ofEchinococcus multilocularisandEchinococcus ortleppi
Authors: Avcioglu Hamza , Guven Esin , Balkaya Ibrahim , Kirman Ridvan , Akyuz Muzaffer , Mebarek Bia Mohammed , Gulbeyen Hatice , Yaya Sali 
Published: May 2021

Abstract
The genomic distribution map of human papillomavirus in Western China
Authors: Chen Ling , Dong Yan , Li Jiao , Zhao Jinqiu , Wang Dan , Xu Li , Wu Yue , Liu Huandong , Lu Jungao , Yao Zuoyi , Li Xiaosong 
Published: May 2021

Abstract
Human papillomavirus (HPV) has been confirmed as the causative agent for cervical cancer. In this study, a total of 301 880 women were recruited from four different regions of Western China, with 301 880 exfoliated cervical cell samples collected from women for DNA isolation and purification. The HPV genotype was tested by polymerase chain reaction. The overall HPV prevalence rate, high-risk (HR) HPV infection rate, low-risk (LR) HPV infection rate and mixed HPV infection rate was 18.24%, 79.14%, 12.56% and 8.30%, respectively. The four most common HR HPV subtypes were HPV-52, 16, 58 and 53, which accounted for 20.49%, 19.93%, 14.54% and 10.01%, respectively. In LR HPV genotype, HPV-6 ranked the highest (28.17%), followed by HPV-81 (9.09%) and HPV-11 (3.78%). HPV genotype subgroup analysis also showed that single-type infection was the most common (77.26%) among HPV-positive individuals. Among multi-infection genotypes, double infection was the most common with frequencies of 76.04%. The overall prevalence of HPV is high in Western China, whose distribution demonstrates different patterns across different ages and regions. Viral genotypes HPV 53, 6 were frequently detected in this population, which is worth of significant clinical attention.
Breeding behaviour of colour-aberrant Adélie penguins (Pygoscelis adeliae) at Cape Crozier, Ross Island, Antarctica
Authors: Levinson Parker M. , Schmidt Annie E. , Morandini Virginia , Elrod Megan , Jongsomjit Dennis , Ballard Grant 
Published: May 2021

AbstractPlumage colour variation occurs widely among bird species and is often associated with individual fitness. More specifically, colouration can affect thermoregulatory ability, mate selection and conspicuousness during foraging. Colour aberrations can be caused by genetic mutations, dietary imbalances, environmental conditions or disease and are rare. Plumage variations have previously been noted in Adélie penguins, although without any follow-up to measure implications for behaviour or fitness. To assess how this low-frequency condition affects breeding in Adélie penguins, we monitored the breeding of several colour-aberrant Adélie penguins during the 2019–2020 nesting season at the large Cape Crozier, Ross Island colony (> 300,000 pairs). In total, we found 12 individuals with unusual plumage for a frequency of 1:50,000 breeding penguins. There were seven dark brown Adélie penguins, three progressive greying Adélie penguins, one dilute Adélie penguin and one brown Adélie penguin, of which five were female, three male and four of unknown sex. Six colour aberrants initiated breeding with a normal-coloured mate, and five raised at least one chick to crèche. The likelihood of breeding and breeding success of colour aberrants were similar to those of normal-coloured Adélie penguins, suggesting that colour aberrations do not negatively affect breeding.
Snowball Sampling and Facebook: How Social Media Can Help Access Hard-to-Reach Populations
Authors: Dosek Tomas 
Published: May 2021

ABSTRACTThe snowball sampling method (SSM) is one of the most widely used methods to collect information about hard-to-reach populations and in low-information contexts. This article argues that SSM can be enhanced by the use of Facebook in at least three ways. This social-networking site helps to address some of the inherent limitations of SSM by learning about local contexts and identifying principal political actors, by gathering background information about and establishing contact with them, and by triangulating information they provide and reaching out to additional key informants. Thus, this article shows how Facebook can provide leverage in overcoming the problem of biased samples and offers concrete empirical illustrations from research on local politics in Latin American countries.
District magnitude and electoral mobilization: how uneven electoral systems shift the focus of campaign efforts by political parties
Authors: Umeda Michio 
Published: May 2021

AbstractThis paper shows how an uneven electoral system in Japan shapes political parties' mobilization strategies by utilizing a majoritarian electoral system with heterogeneous district magnitudes, which in turn contributes to the gap in turnout across districts. Scholars have long debated the relationship between electoral systems and turnout; it is known that countries with proportional representation electoral systems – those with larger district magnitude – tend to have higher turnout rates than countries with majoritarian electoral systems, especially single-member district (SMD) systems. The current discussion on turnout and district magnitude of an electoral system assumes a monotonic relationship between these factors: the larger the district magnitude of the electoral system, the more (or less) participatory the electorate, due to competitiveness and mobilization efforts by political parties and other relevant groups. In contrast, this paper shows a mixed relationship between district magnitude of the electoral system and party mobilization and subsequent turnout, investigating a majoritarian electoral system with uneven district magnitude in the Japanese Upper House. During the survey period, the party system in Japan consisted of two major parties and a few smaller parties; consequently, the two major parties focused their efforts on SMDs in order to maximize their seat share, while smaller parties focused their resource on districts electing more than two members (where they have some chance to elect their party's candidates). In combination, these party strategies have resulted in the lowest mobilization and turnout rates in districts with two members.
Localized Scenarios and Latitudinal Patterns of Vertical and Lateral Resilience of Tidal Marshes to Sea‐Level Rise in the Contiguous United States
Authors: Holmquist James R. , Brown Lauren N. , MacDonald Glen M. 
Published: May 2021

AbstractCoastal wetlands have two dimensions of vulnerability to sea‐level rise (SLR), a vertical one, in cases where SLR outpaces their capacity to vertically accrete, and a lateral one, in cases where they are restricted from migrating inland by topography and land use. We conducted a meta‐analysis of accretion rates, standardized our analysis by using only 137Cs based estimates, and used model intercomparison to generate a vertical resilience index, a function of local SLR, tidal range, and tidal elevation category for the tidal wetlands of the contiguous US. We paired the vertical resilience index with a lateral resilience index made up of elevation, water level, and land cover maps, then projected them both into the future using localized SLR scenarios. At the regional scale, the vertical resilience index predicts changes from marsh aggradation to submergence for the coastal US Mid‐Atlantic, Southeast, and portions of the Northeast by 2100. At the sub‐regional scale, there is a geographic tradeoff between vertical and lateral resilience with more northerly wetlands vulnerable to the lack of suitable proportional area to migrate into, and more southerly wetlands vulnerable to accretion deficit. We estimate between 43% and 48% of the existing contiguous US wetland area, almost entirely located in watersheds along the Gulf of Mexico and Mid‐Atlantic coasts, is subject to both vertical and lateral limitations. These vertical and lateral resilience indices could help direct future research, planning, and mitigation efforts at a national scale, as well as supplement more processed informed approaches by local planners and practitioners.
Inflammatory responses to metal oxide ceramic nanopowders
Authors: Jamieson Shannon , Mawdesley Amy , Deehan David , Kirby John , Holland James , Tyson-Capper Alison 
Published: May 2021

AbstractCeramic orthopaedic implants are increasingly popular due to the need for robust total joint replacement implants that have a high success rate long-term and do not induce biological responses in patients. This study was designed to investigate the biological effects of ceramic nanopowders containing aluminium oxide or zirconium oxide to activate the human macrophage THP-1 cell line. In vitro investigation of pro-inflammatory gene expression and chemokine secretion was performed studied using RT-qPCR and ELISA, respectively. TLR4 inhibition, using a small-molecule inhibitor, was used to determine whether ceramic-mediated inflammation occurs in a similar manner to that of metals such as cobalt. THP-1 macrophages were primed with ceramics or LPS and then treated with ATP or ceramics, respectively, to determine whether these nanopowders are involved in the priming or activation of the NLRP3 inflammasome through IL-1β secretion. Cells treated with ceramics significantly increased pro-inflammatory gene expression and protein secretion which was attenuated through TLR4 blockade. Addition of ATP to cells following ceramic treatment significantly increased IL-1β secretion. Therefore, we identify the ability of ceramic metal oxides to cause a pro-inflammatory phenotype in THP-1 macrophages and propose the mechanism by which this occurs is primarily via the TLR4 pathway which contributes to inflammasome signalling.
Predicting language treatment response in bilingual aphasia using neural network-based patient models
Authors: Grasemann Uli , Peñaloza Claudia , Dekhtyar Maria , Miikkulainen Risto , Kiran Swathi 
Published: May 2021

AbstractPredicting language therapy outcomes in bilinguals with aphasia (BWA) remains challenging due to the multiple pre- and poststroke factors that determine the deficits and recovery of their two languages. Computational models that simulate language impairment and treatment outcomes in BWA can help predict therapy response and identify the optimal language for treatment. Here we used the BiLex computational model to simulate the behavioral profile of language deficits and treatment response of a retrospective sample of 13 Spanish-English BWA who received therapy in one of their languages. Specifically, we simulated their prestroke naming ability and poststroke naming impairment in each language, and their treatment response in the treated and the untreated language. BiLex predicted treatment effects accurately and robustly in the treated language and captured different degrees of cross-language generalization in the untreated language in BWA. Our cross-validation approach further demonstrated that BiLex generalizes to predict treatment response for patients whose data were not used in model training. These findings support the potential of BiLex to predict therapy outcomes for BWA and suggest that computational modeling may be helpful to guide individually tailored rehabilitation plans for this population.
Kokumi taste perception is functional in a model carnivore, the domestic cat (Felis catus)
Authors: Laffitte A. , Gibbs M. , Hernangomez de Alvaro C. , Addison J. , Lonsdale Z. N. , Giribaldi M. G. , Rossignoli A. , Vennegeerts T. , Winnig M. , Klebansky B. , Skiles J. , Logan D. W. , McGrane S. J. 
Published: May 2021

AbstractKokumi taste is a well-accepted and characterised taste modality and is described as a sensation of enhancement of sweet, salty, and umami tastes. The Calcium Sensing Receptor (CaSR) has been designated as the putative kokumi taste receptor for humans, and a number of kokumi-active ligands of CaSR have been discovered recently with activity confirmed both in vivo and in vitro. Domestic cats (Felis catus) are obligate carnivores and accordingly, their diet is abundant in proteins, peptides, and amino acids. We hypothesised that CaSR is a key taste receptor for carnivores, due to its role in the detection of different peptides and amino acids in other species. Using in silico, in vitro and in vivo approaches, here we compare human CaSR to that of a model carnivore, the domestic cat. We found broad similarities in ligand specificity, but differences in taste sensitivity between the two species. Indeed our in vivo data shows that cats are sensitive to CaCl2 as a kokumi compound, but don’t show this same activity with Glutathione, whereas for humans the reverse is true. Collectively, our data suggest that kokumi is an important taste modality for carnivores that drives the palatability of meat-derived compounds such as amino acids and peptides, and that there are differences in the perception of kokumi taste between carnivores and omnivores.
Predictors for affected stroke territory and outcome of acute stroke treatments are different for posterior versus anterior circulation stroke
Authors: Handelsmann H. , Herzog L. , Kulcsar Z. , Luft A. R. , Wegener S. 
Published: May 2021

AbstractDistinct patient characteristics have been proposed for ischaemic stroke in the anterior versus posterior circulation. However, data on functional outcome according to stroke territory in patients with acute stroke treatment are conflicting and information on outcome predictors is scarce. In this retrospective study, we analysed functional outcome in 517 patients with stroke and thrombolysis and/or thrombectomy treated at the University Hospital Zurich. We compared clinical factors and performed multivariate logistic regression analyses investigating the effect of outcome predictors according to stroke territory. Of the 517 patients included, 80 (15.5%) suffered a posterior circulation stroke (PCS). PCS patients were less often female (32.5% vs. 45.5%, p = 0.031), received thrombectomy less often (28.7% vs. 48.3%, p = 0.001), and had lower median admission NIHSS scores (5 vs. 10, p < 0.001) as well as a better median three months functional outcome (mRS 1 vs. 2, p = 0.010). Predictors for functional outcome were admission NIHSS (OR 0.864, 95% CI 0.790–0.944, p = 0.001) in PCS and age (OR 0.952, 95% CI 0.935–0.970, p < 0.001), known symptom onset (OR 1.869, 95% CI 1.111–3.144, p = 0.018) and admission NIHSS (OR 0.840, 95% CI 0.806–0.876, p < 0.001) in ACS. Acutely treated PCS and ACS patients differed in their baseline and treatment characteristics. We identified specific functional outcome predictors of thrombolysis and/or thrombectomy success for each stroke territory.
Evaluation of schistosomula crude antigen (SCA) as a diagnostic tool for Schistosoma mansoni in low endemic human population
Authors: Oyeyemi Oyetunde Timothy , Corsini Camila Amormino , Gonçalves Gustavo , de Castro Borges William , Grenfell Rafaella Fortini Queiroz 
Published: May 2021

AbstractThe study aimed to determine the potential of schistosomula crude antigen (SCA) as a diagnostic target for anti-S. mansoni antibody detection. Cercariae were transformed into schistosomula, homogenized through sonication, and then centrifuged to obtain the SCA. SCA was evaluated using ELISA and dot blots immunoassays on 30 S. mansoni infected sera samples obtained from chronic patients and 30 non-infected humans’ sera samples. Either Kato-Katz or saline gradient method or both were employed as the diagnostic reference. Dot blots immunoassay was further performed on protein eluted from 10 to 12 kDa immunoreactive band identified by Western blot analysis. The area under the ROC curve was 0.95 (AUC 0.95, CI 0.88–1.01, p < 0.0001). The sensitivity and specificity of SCA-ELISA and dot blots assays were 96.67% and 86.67% respectively. The human IgG-specific response against SCA was significantly higher in S. mansoni infected individuals (OD = 0.678 ± 0.249) compared to the non-infected population (OD = 0.235 ± 0.136) (p < 0.0001). Our study showed that SCA and its 10–12 kDa component could be useful as diagnostic tools for chronic schistosomiasis.
Pharmacokinetics of anti-infective agents during CytoSorb hemoadsorption
Authors: Schneider Antoine G. , André Pascal , Scheier Joerg , Schmidt Monika , Ziervogel Heiko , Buclin Thierry , Kindgen-Milles Detlef 
Published: May 2021

AbstractCytokine hemoadsorption might be beneficial in patients with sepsis. However, its effect on anti-infective agents' disposition remains largely unknown. We sought to determine the influence of hemoadsorption on the pharmacokinetics of common anti-infective agents. This is an interventional experimental study, conducted in 24 healthy pigs. Animals were randomly allocated to either hemoadsorption (cases) or sham extracorporeal circuit (controls) and to drug combinations (3 cases and 3 controls for each combination). Hemoadsorption was performed with CytoSorb (CytoSorbents Corporation, USA). We evaluated 17 drugs (clindamycin, fluconazole, linezolid, meropenem, piperacillin, anidulafungin, ganciclovir, clarithromycin, posaconazole, teicoplanin, tobramycin, ceftriaxone, ciprofloxacin, metronidazole, liposomal amphotericin B, flucloxacillin and cefepime). Repeated blood sampling from the extracorporeal circulation (adsorber inlet/outlet, sham circuit) was performed over six hours following administration. Total clearance and adsorber-specific clearance were computed. Hemoadsorption was associated with increased clearance of all study drugs, except ganciclovir. Its impact on total body clearance was considered as moderate for fluconazole (282%) and linezolid (115%), mild for liposomal amphotericin B (75%), posaconazole (32%) and teicoplanine (31%) and negligible for all other drugs. Hemoadsorber clearance declined over time, with even delayed desorption for beta-lactams. It was moderately correlated with drug's lipophilicity (p = 0.01; r2 = 0.43). Hemoadsorption with CytoSorb appears to increase to a clinically significant extent the clearance of five among 17 tested anti-infectives. Studies in human patients are required to confirm the need for dosage adjustment of these agents.
Visual motion integration of bidirectional transparent motion in mouse opto-locomotor reflexes
Authors: Kirkels L. A. M. H. , Zhang W. , Rezvani Z. , van Wezel R. J. A. , van Wanrooij M. M. 
Published: May 2021

AbstractVisual motion perception depends on readout of direction selective sensors. We investigated in mice whether the response to bidirectional transparent motion, activating oppositely tuned sensors, reflects integration (averaging) or winner-take-all (mutual inhibition) mechanisms. We measured whole body opto-locomotor reflexes (OLRs) to bidirectional oppositely moving random dot patterns (leftward and rightward) and compared the response to predictions based on responses to unidirectional motion (leftward or rightward). In addition, responses were compared to stimulation with stationary patterns. When comparing OLRs to bidirectional and unidirectional conditions, we found that the OLR to bidirectional motion best fits an averaging model. These results reflect integration mechanisms in neural responses to contradicting sensory evidence as has been documented for other sensory and motor domains.
AAV-mediated YAP expression in cardiac fibroblasts promotes inflammation and increases fibrosis
Authors: Francisco Jamie , Zhang Yu , Nakada Yasuki , Jeong Jae Im , Huang Chun-Yang , Ivessa Andreas , Oka Shinichi , Babu Gopal J. , Del Re Dominic P. 
Published: May 2021

AbstractFibrosis is a hallmark of heart disease independent of etiology and is thought to contribute to impaired cardiac dysfunction and development of heart failure. However, the underlying mechanisms that regulate the differentiation of fibroblasts to myofibroblasts and fibrotic responses remain incompletely defined. As a result, effective treatments to mitigate excessive fibrosis are lacking. We recently demonstrated that the Hippo pathway effector Yes-associated protein (YAP) is an important mediator of myofibroblast differentiation and fibrosis in the infarcted heart. Yet, whether YAP activation in cardiac fibroblasts is sufficient to drive fibrosis, and how fibroblast YAP affects myocardial inflammation, a significant component of adverse cardiac remodeling, are largely unknown. In this study, we leveraged adeno-associated virus (AAV) to target cardiac fibroblasts and demonstrate that chronic YAP expression upregulated indices of fibrosis and inflammation in the absence of additional stress. YAP occupied the Ccl2 gene and promoted Ccl2 expression, which was associated with increased macrophage infiltration, pro-inflammatory cytokine expression, collagen deposition, and cardiac dysfunction in mice with cardiac fibroblast-targeted YAP overexpression. These results are consistent with other recent reports and extend our understanding of YAP function in modulating fibrotic and inflammatory responses in the heart.
Regeneration linked miRNA modify tumor phenotype and can enforce multi-lineage growth arrest in vivo
Authors: Salehi Siamak , Tavabie Oliver D. , Villanueva Augusto , Watson Julie , Darling David , Quaglia Alberto , Farzaneh Farzin , Aluvihare Varuna R. 
Published: May 2021

AbstractRegulated cell proliferation is an effector mechanism of regeneration, whilst dysregulated cell proliferation is a feature of cancer. We have previously identified microRNA (miRNA) that regulate successful and failed human liver regeneration. We hypothesized that these regulators may directly modify tumor behavior. Here we show that inhibition of miRNAs -503 and -23a, alone or in combination, enhances tumor proliferation in hepatocyte and non-hepatocyte derived cancers in vitro,driving more aggressive tumor behavior in vivo. Inhibition of miRNA-152 caused induction of DNMT1, site-specific methylation with associated changes in gene expression and in vitro and in vivo growth inhibition. Enforced changes in expression of two miRNA recapitulating changes observed in failed regeneration led to complete growth inhibition of multi-lineage cancers in vivo. Our results indicate that regulation of regeneration and tumor aggressiveness are concordant and that miRNA-based inhibitors of regeneration may constitute a novel treatment strategy for human cancers.
Scrutinizing the stability and exploring the dependence of thermoelectric properties on band structure of 3d-3d metal-based double perovskites Ba2FeNiO6 and Ba2CoNiO6
Authors: Mir Shabir Ahmad , Gupta Dinesh C. 
Published: May 2021

AbstractThrough the conventional DFT computation, we have designed new oxide double perovskites Ba2FeNiO6 and Ba2CoNiO6. The structural and thermodynamic stabilities are predicted by optimizing the crystal structure and evaluation of enthalpy of formation, respectively. Then by using the optimized lattice constant, we have explored the different physical properties. The GGA + mBJ electronic band-structure illustrates Ba2FeNiO6 is a half-metal with 100% spin polarization at the Fermi level. While Ba2CoNiO6 shows a ferromagnetic semiconducting nature. The change in the electronic structure when Fe is replaced by Co is explained with the help of the orbital diagram and exchange interaction. The eg-eg hybridization that happens via O-p states is strong because Fe–O–Ni and Co–O–Ni bond angles are strictly 180°. The narrow bandgaps in the semiconducting channels prompted us to analyze the applicability of these materials towards thermoelectric technology. Besides this, we have investigated the dependency of transport properties on electronic band structure. The semiconducting nature in Ba2CoNiO6 results in a significant ZT around 0.8 at room temperature makes it suitable for wasted-energy regeneration