bluuebunny/crossref_metadata_2025_split huggingface dataset:
Optimization of α‐amylase and Glucoamylase Production Using Cull Potatoes as Substrate
Authors: Bansal Riya , Katyal Priya 
Published: May 2021

AbstractAmylases are required for the conversion of starchy substrate into sugars. Commercially available enzymes are quite costly which makes the process uneconomical. This study is conducted to standardize physico‐chemical parameters such as substrate concentration (1.0–5.0%), temperature (25–40 °C), pH (4.0–7.0), incubation time (18–24) h for α‐amylase and 3–6 days for glucoamylase production) for optimum production of α‐amylase from bacterial isolate (AM1) and glucoamylase from Aspergillus niger by using cull potatoes as a substrate. Various organic and inorganic nitrogen sources (urea, yeast extract and soybean meal, and ammonium sulphate) are tried for maximum enzyme production. Among the four nitrogen sources, yeast extract supported maximum α‐amylase and glucoamylase production. Among the different amylolytic isolates from fermented jalebi dough and AM1culture, maximum α‐amylase activity is shown by AM1 (90.46 U mL−1) as compared to J1 (71.02 U mL−1). After optimization, α‐amylase activity of AM1 increases by 41.73% from 198.20 to 280.92 U mL−1 at 5.0% substrate concentration, 30 °C temperature, 24 h of incubation and pH 5.0. Glucoamylase activity of A. niger increases by 38.78% from 228.72 to 317.44 U mL−1 after optimization at 5% substrate concentration, pH 5, incubation period of 4 days and temperature 30 °C.
Reluctantly Solo? Representations of Single Mothers via Donor Procedure, Insemination and IVF in Swedish Newspapers
Authors: Wahlström Henriksson Helena , Bergnehr Disa 
Published: May 2021

AbstractThis chapter explores representations of single mothers via ARTs (assisted reproductive technologies) in Swedish newspapers. These women are gender-politically interesting to study because they stand as examples of ‘families we choose’ that subvert the hegemony of the nuclear family ideal, and they activate pertinent questions concerning reproductive choices and practices. The mothers in the news articles are typically white, heterosexual and urban, and they, their parenthood and ‘choice’, are framed in particular ways to project maternal and feminine respectability as well as ‘intensive mothering’. This suggests that, although in an international comparison there is generally little stigma attached to single motherhood in Sweden, these mothers need to become ‘understandable’ as good mothers in specific ways to deflect (risk of) stigma.
Cosmological Cutting Rules
Authors: Melville Scott , Pajer Enrico 
Published: May 2021

Abstract
Primordial perturbations in our universe are believed to have a quantum origin, and can be described by the wavefunction of the universe (or equivalently, cosmological correlators). It follows that these observables must carry the imprint of the founding principle of quantum mechanics: unitary time evolution. Indeed, it was recently discovered that unitarity implies an infinite set of relations among tree-level wavefunction coefficients, dubbed the Cosmological Optical Theorem. Here, we show that unitarity leads to a systematic set of “Cosmological Cutting Rules” which constrain wavefunction coefficients for any number of fields and to any loop order. These rules fix the discontinuity of an n-loop diagram in terms of lower-loop diagrams and the discontinuity of tree-level diagrams in terms of tree-level diagrams with fewer external fields. Our results apply with remarkable generality, namely for arbitrary interactions of fields of any mass and any spin with a Bunch-Davies vacuum around a very general class of FLRW spacetimes. As an application, we show how one-loop corrections in the Effective Field Theory of inflation are fixed by tree-level calculations and discuss related perturbative unitarity bounds. These findings greatly extend the potential of using unitarity to bootstrap cosmological observables and to restrict the space of consistent effective field theories on curved spacetimes.
Classical solutions and their double copy in split signature
Authors: Monteiro Ricardo , O’Connell Donal , Veiga David Peinador , Sergola Matteo 
Published: May 2021

AbstractThe three-point amplitude is the key building block in the on-shell approach to scattering amplitudes. We show that the classical objects computed by massive three-point amplitudes in gauge theory and gravity are Newman-Penrose scalars in a split-signature spacetime, where three-point amplitudes can be defined for real kinematics. In fact, the quantum state set up by the particle is a coherent state fully determined by the three-point amplitude due to an eikonal-type exponentiation. Having identified this simplest classical solution from the perspective of scattering amplitudes, we explore the double copy of the Newman-Penrose scalars induced by the traditional double copy of amplitudes, and find that it coincides with the Weyl version of the classical double copy. We also exploit the Kerr-Schild version of the classical double copy to determine the exact spacetime metric in the gravitational case. Finally, we discuss the direct implication of these results for Lorentzian signature via analytic continuation.
Gopakumar-Vafa hierarchies in winding inflation and uplifts
Authors: Carta Federico , Mininno Alessandro , Righi Nicole , Westphal Alexander 
Published: May 2021

Abstract
We propose a combined mechanism to realize both winding inflation and de Sitter uplifts. We realize the necessary structure of competing terms in the scalar potential not via tuning the vacuum expectation values of the complex structure moduli, but by a hierarchy of the Gopakumar-Vafa invariants of the underlying Calabi-Yau threefold. To show that Calabi-Yau threefolds with the prescribed hierarchy actually exist, we explicitly create a database of all the genus 0 Gopakumar-Vafa invariants up to total degree 10 for all the complete intersection Calabi-Yau’s up to Picard number 9. As a side product, we also identify all the redundancies present in the CICY list, up to Picard number 13. Both databases can be accessed at this link (https://www.desy.de/∼westphal/GV_CICY_webpage/GVInvariants.html).
On small black holes, KK monopoles and solitonic 5-branes
Authors: Cano Pablo A. , Murcia Ángel , Ramírez Pedro F. , Ruipérez Alejandro 
Published: May 2021

Abstract
We review and extend results on higher-curvature corrections to different configurations describing a superposition of heterotic strings, KK monopoles, solitonic 5-branes and momentum waves. Depending on which sources are present, the low-energy fields describe a black hole, a soliton or a naked singularity. We show that this property is unaltered when perturbative higher-curvature corrections are included, provided the sources are fixed. On the other hand, this character may be changed by appropriate introduction (or removal) of sources regardless of the presence of curvature corrections, which constitutes a non-perturbative modification of the departing system. The general system of multicenter KK monopoles and their 5-brane charge induced by higher-curvature corrections is discussed in some detail, with special attention paid to the possibility of merging monopoles. Our results are particularly relevant for small black holes (Dabholkar-Harvey states, DH), which remain singular after quadratic curvature corrections are taken into account. When there are four non-compact dimensions, we notice the existence of a black hole with regular horizon whose entropy coincides with that of the DH states, but the charges and supersymmetry preserved by both configurations are different. A similar construction with five non-compact dimensions is possible, in this case with the same charges as DH, although it fails to reproduce the DH entropy and supersymmetry. No such configuration exists if d > 5, which we interpret as reflecting the necessity of having a 5-brane wrapping the compact space.
On Gauss-Bonnet gravity and boundary conditions in Lorentzian path-integral quantization
Authors: Narain Gaurav 
Published: May 2021

Abstract
Recently there has been a surge of interest in studying Lorentzian quant urn cosmology using Picard-Lefschetz methods. The present paper aims to explore the Lorentzian path-integral of Gauss-Bonnet gravity in four spacetime dimensions with metric as the field variable. We employ mini-superspace approximation and study the variational problem exploring different boundary conditions. It is seen that for mixed boundary conditions non-trivial effects arise from Gauss-Bonnet sector of gravity leading to additional saddle points for lapse in some case. As an application of this we consider the No-boundary proposal of the Universe with two different settings of boundary conditions) and compute the transition amplitude using Picard-Lefschetz formalism. In first case the transition amplitude is a superposition of a Lorentzian and a Euclidean geometrical configuration leading to interference incorporating non-perturbative effects coming from Gauss-Bonnet sector of gravity. In the second case involving complex initial momentum we note that the transition amplitude is an analogue of Hartle-Hawking wave-function with non-perturbative correction coming from Gauss-Bonnet sector of gravity.
The Cosmological Semiclassical Einstein Equation as an Infinite-Dimensional Dynamical System
Authors: Gottschalk Hanno , Siemssen Daniel 
Published: May 2021

AbstractWe develop a comprehensive framework in which the existence of solutions to the semiclassical Einstein equation (SCE) in cosmological spacetimes is shown. Different from previous work on this subject, we do not restrict to the conformally coupled scalar field and we admit the full renormalization freedom. Based on a regularization procedure, which utilizes homogeneous distributions and is equivalent to Hadamard point splitting, we obtain a reformulation of the evolution of the quantum state as an infinite-dimensional dynamical system with mathematical features that are distinct from the standard theory of infinite-dimensional dynamical systems (e.g., unbounded evolution operators). Nevertheless, applying methods closely related to Ovsyannikov’s method, we show existence of maximal/global solutions to the SCE for vacuum-like states and of local solutions for thermal-like states. Our equations do not show the instability of the Minkowski solution described by other authors.
Extracellular matrix remodeling in animal models of anthracycline-induced cardiomyopathy: a meta-analysis
Authors: Leerink Jan M. , van de Ruit Mabel , Feijen Elizabeth A.M. , Kremer Leontien C.M. , Mavinkurve-Groothuis Annelies M.C. , Pinto Yigal M. , Creemers Esther E. , Kok Wouter E.M. 
Published: May 2021

AbstractAs in other cardiomyopathies, extracellular matrix (ECM) remodeling plays an important role in anthracycline-induced cardiomyopathy. To understand the pattern and timing of ECM remodeling pathways, we conducted a systematic review in which we describe protein and mRNA markers for ECM remodeling that are differentially expressed in the hearts of animals with anthracycline-induced cardiomyopathy. We included 68 studies in mice, rats, rabbits, and pigs with follow-up of 0.1–8.2 human equivalent years after anthracycline administration. Using meta-analysis, we found 29 proteins and 11 mRNAs that were differentially expressed in anthracycline-induced cardiomyopathy compared to controls. Collagens, matrix metalloproteinases (MMPs), inflammation markers, transforming growth factor ß signaling markers, and markers for cardiac hypertrophy were upregulated, whereas the protein kinase B (AKT) pro-survival pathway was downregulated. Their expression patterns over time from single time point studies were studied with meta-regression using human equivalent years as the time scale. Connective tissue growth factor showed an early peak in expression but remained upregulated at all studied time points. Brain natriuretic peptide (BNP) and MMP9 protein levels increased in studies with longer follow-up. Significant associations were found for higher atrial natriuretic peptide with interstitial fibrosis and for higher BNP and MMP2 protein levels with left ventricular systolic function.
Proteomic profiling of murine biliary-derived hepatic organoids and their capacity for drug disposition, bioactivation and detoxification
Authors: Howell Lawrence , Jenkins Rosalind E. , Lynch Stephen , Duckworth Carrie , Kevin Park B. , Goldring Christopher 
Published: May 2021

AbstractHepatic organoids are a recent innovation in in vitro modeling. Initial studies suggest that organoids better recapitulate the liver phenotype in vitro compared to pre-existing proliferative cell models. However, their potential for drug metabolism and detoxification remains poorly characterized, and their global proteome has yet to be compared to their tissue of origin. This analysis is urgently needed to determine what gain-of-function this new model may represent for modeling the physiological and toxicological response of the liver to xenobiotics. Global proteomic profiling of undifferentiated and differentiated hepatic murine organoids and donor-matched livers was, therefore, performed to assess both their similarity to liver tissue, and the expression of drug-metabolizing enzymes and transporters. This analysis quantified 4405 proteins across all sample types. Data are available via ProteomeXchange (PXD017986). Differentiation of organoids significantly increased the expression of multiple cytochrome P450, phase II enzymes, liver biomarkers and hepatic transporters. While the final phenotype of differentiated organoids is distinct from liver tissue, the organoids contain multiple drug metabolizing and transporter proteins necessary for liver function and drug metabolism, such as cytochrome P450 3A, glutathione-S-transferase alpha and multidrug resistance protein 1A. Indeed, the differentiated organoids were shown to exhibit increased sensitivity to midazolam (10–1000 µM) and irinotecan (1–100 µM), when compared to the undifferentiated organoids. The predicted reduced activity of HNF4A and a resulting dysregulation of RNA polymerase II may explain the partial differentiation of the organoids. Although further experimentation, optimization and characterization is needed relative to pre-existing models to fully contextualize their use as an in vitro model of drug-induced liver injury, hepatic organoids represent an attractive novel model of the response of the liver to xenobiotics. The current study also highlights the utility of global proteomic analyses for rapid and accurate evaluation of organoid-based test systems.
Preoperative breast magnetic resonance imaging in patients with ductal carcinoma in situ: a systematic review for the European Commission Initiative on Breast Cancer (ECIBC)
Authors: Canelo-Aybar Carlos , Taype-Rondan Alvaro , Zafra-Tanaka Jessica Hanae , Rigau David , Graewingholt Axel , Lebeau Annette , Pérez Gómez Elsa , Rossi Paolo Giorgi , Langendam Miranda , Posso Margarita , Parmelli Elena , Saz-Parkinson Zuleika , Alonso-Coello Pablo 
Published: May 2021

Abstract
Objective
To evaluate the impact of preoperative MRI in the management of Ductal carcinoma in situ (DCIS).

Methods
We searched the PubMed, EMBASE and Cochrane Library databases to identify randomised clinical trials (RCTs) or cohort studies assessing the impact of preoperative breast MRI in surgical outcomes, treatment change or loco-regional recurrence. We provided pooled estimates for odds ratios (OR), relative risks (RR) and proportions and assessed the certainty of the evidence using the GRADE approach.

Results
We included 3 RCTs and 23 observational cohorts, corresponding to 20,415 patients. For initial breast-conserving surgery (BCS), the RCTs showed that MRI may result in little to no difference (RR 0.95, 95% CI 0.90 to 1.00) (low certainty); observational studies showed that MRI may have no difference in the odds of re-operation after BCS (OR 0.96; 95% CI 0.36 to 2.61) (low certainty); and uncertain evidence from RCTs suggests little to no difference with respect to total mastectomy rate (RR 0.91; 95% CI 0.65 to 1.27) (very low certainty). We also found that MRI may change the initial treatment plans in 17% (95% CI 12 to 24%) of cases, but with little to no effect on locoregional recurrence (aHR = 1.18; 95% CI 0.79 to 1.76) (very low certainty).

Conclusion
We found evidence of low to very low certainty which may suggest there is no improvement of surgical outcomes with pre-operative MRI assessment of women with DCIS lesions. There is a need for large rigorously conducted RCTs to evaluate the role of preoperative MRI in this population.

Key Points
• Evidence of low to very low certainty may suggest there is no improvement in surgical outcomes with pre-operative MRI.
• There is a need for large rigorously conducted RCTs evaluating the role of preoperative MRI to improve treatment planning for DCIS.
Thermally tolerant symbionts may explain Caribbean octocoral resilience to heat stress
Authors: Pelosi Jessie , Eaton Katherine M. , Mychajliw Samantha , terHorst Casey P. , Coffroth Mary Alice 
Published: May 2021

AbstractCoral reef ecosystems are under threat from the frequent and severe impacts of anthropogenic climate change, particularly rising sea surface temperatures. The effects of thermal stress may be ameliorated by adaptation and/or acclimation of the host, symbiont, or holobiont (host + symbiont) to increased temperatures. We examined the role of the symbiont in promoting thermal tolerance of the holobiont, using Antillogorgia bipinnata (octocoral host) and Breviolum antillogorgium (symbiont) as a model system. We identified five distinct genotypes of B. antillogorgium from symbiont populations isolated from Antillogorgia colonies in the Florida Keys. Three symbiont genotypes were cultured and maintained at 26 °C (ambient historical temperature), and two were cultured and maintained at 30 °C (elevated historical temperature) for 2 yrs. We analyzed the growth rate and carrying capacity of each symbiont genotype at both ambient and elevated temperatures in culture (in vitro). All genotypes grew well at both temperatures, indicating that thermal tolerance exists among these B. antillogorgium cultures. However, a history of long-term growth at 30 °C did not yield better performance for B. antillogorgium at 30 °C (as compared to 26 °C), suggesting that prior culturing at the elevated temperature did not result in increased thermal tolerance. We then inoculated juvenile A. bipinnata polyps with each of the five symbiont genotypes and reared these polyps at both ambient and elevated temperatures (in hospite experiment). All genotypes established symbioses with polyps in both temperature treatments. Survivorship of polyps at 30 °C was significantly lower than survivorship at 26 °C, but all treatments had surviving polyps at 56 d post-infection. Our results suggest broad thermal tolerance in B. antillogorgium, which may play a part in the increased resilience of Caribbean octocorals during heat stress events.
Exploiting large ensembles for a better yet simpler climate model evaluation
Authors: Suarez-Gutierrez Laura , Milinski Sebastian , Maher Nicola 
Published: May 2021

AbstractWe use a methodological framework exploiting the power of large ensembles to evaluate how well ten coupled climate models represent the internal variability and response to external forcings in observed historical surface temperatures. This evaluation framework allows us to directly attribute discrepancies between models and observations to biases in the simulated internal variability or forced response, without relying on assumptions to separate these signals in observations. The largest discrepancies result from the overestimated forced warming in some models during recent decades. In contrast, models do not systematically over- or underestimate internal variability in global mean temperature. On regional scales, all models misrepresent surface temperature variability over the Southern Ocean, while overestimating variability over land-surface areas, such as the Amazon and South Asia, and high-latitude oceans. Our evaluation shows that MPI-GE, followed by GFDL-ESM2M and CESM-LE offer the best global and regional representation of both the internal variability and forced response in observed historical temperatures.
Intraocular epidermal growth factor concentration, axial length, and high axial myopia
Authors: Jonas Jost B. , Dong Li , Da Chen Shi , Neumaier Michael , Findeisen Peter , Panda-Jonas Songhomitra , Jonas Rahul A. 
Published: May 2021

AbstractPurposeVarious molecules such as dopamine have been found to be associated with axial elongation in experimental studies. Here, we examined whether intraocular EGF is associated with axial length in myopic patients.MethodsThe hospital-based investigation included patients of European descent without optic nerve, retinal, or macular diseases except for myopic maculopathy. Using aqueous humor samples collected during surgery, the EGF concentration was examined applying a cytometric bead array. High myopia was defined by an axial length of ≥ 27.0 mm.ResultsThe study included a non-highly myopic group of 11 patients (mean age, 72.9 ± 10.8 years; mean axial length, 24.3 ± 1.1 mm) and a highly myopic group of three patients (age, 81.11 ± 12.3 years; axial length, 29.5 ± 1.3 mm), with one of them having pathologic myopic maculopathy. In multivariable linear regression analysis, higher EGF concentration was correlated with the highly myopic versus non-highly myopic group (beta, 1.24; non-standardized correlation coefficient B, 6.24; 95% confidence interval (CI), 0.10,12.4;P = 0.047) after adjusting for axial length. The amount of intraocular EGF was significantly higher in the highly myopic group than in the non-highly myopic group (89.1 ± 40.8 pg versus 34.1 ± 13.2 pg;P = 0.005), and it was highest in the eye with myopic maculopathy (135 pg).ConclusionsThe intraocular amount of EGF is higher in highly myopic versus non-highly myopic eyes.
In Colore Veritas? Color effects on the speed and accuracy of true/false responses
Authors: Nadarevic Lena , Symeonidou Nikoletta , Kias Alina 
Published: May 2021

AbstractIn addition to their perceptual or aesthetic function, colors often carry conceptual meaning. In quizzes, for instance, true and false answers are typically marked in green and red. In three experiments, we used a Stroop task to investigate automatic green-true associations and red-false associations, respectively. In Experiments 1 and 2, stimuli were true statements (e.g., “tables are furniture”) and false statements (e.g., “bananas are buildings”) that were displayed in different combination of green, red, and gray depending on the experimental condition. In Experiment 3, we used true-related and false-related words shown in green, red, or gray. Participants had to indicate the validity (or semantic meaning) of each statement (or word) as fast and as accurately as possible. We expected that participants would perform best when they had to categorize green stimuli as “true” and red stimuli as “false”. The prediction was only confirmed when green and red stimuli were presented within the same context (i.e., same experimental condition). This finding supports the dimension-specificity hypothesis which states that cross-modal associations (here: associations between color and validity) depend on the context (here: the color-context). Moreover, the observed color-validity effects were stronger when participants had to categorize single words instead of sentences and when they had to provide speeded responses. Taken together, these results suggest that controlled processing counteracts the influence of automatic color associations on true/false responses.