bluuebunny/crossref_metadata_2025_split huggingface dataset:
Educação em Direitos Humanos em debate
Authors: Fernandes Ana , Lopes Igreja Beatriz , Rego Carlos Eduardo , Gomes Gabriela , Figueiredo Ivanilda , Oliveira Natalia , Passos Pâmella , Ferreira Vinicius 
Published: January 2021

Transcrição do debate Educação em Direitos Humanos realizado em 16 de setembro de 2020, em uma live durante a Pandemia de Covid-19, através da parceria entre o LAEDH – Laboratório de Educação em Direitos Humanos do Colégio Pedro II e a Agência Espaço e Vida, que contou com as participações de Natália Braga Oliveira, professora de Sociologia do Colégio Pedro II e colaboradora do LAEDH; Vinicius Cavalcanti Ferreira, professor de Geografia da Educação Básica vinculado à à Espaço e Vida, que realiza atividades de turismo pedagógico e atividades de campo, voltadas para alunos do Ensino Fundamental e do Ensino Médio, e que mantém um canal no Youtube, que possibilitou a transmissão do presente debate em seu programa semanal, o Roda Espaço e Vida; Pâmella Passos, professora de História do Instituto Federal de Educação, Ciência e Tecnologia do Rio de Janeiro (IFRJ), onde integra o GPTEC - Grupo de Pesquisa em Tecnologia, Educação & Cultura, que possui produção voltada para práticas de Educação em Direitos Humanos e Ivanilda Figueiredo, professora de Direitos Humanos da Faculdade de Direito da Universidade do Estado do Rio de Janeiro (UERJ), onde integra o URDIR – Núcleo Multidisciplinar de Ensino, Pesquisa e Extensão Universidade, Resistência e Direitos Humanos, no âmbito do qual possui iniciativas para Educação em Direitos Humanos. Este debate foi transcrito por Carlos Eduardo Oliva de C. Rêgo, professor de Sociologia do Colégio Pedro II e integrante do LAEDH, e por Ana Fernandes, Beatriz Lopes Igreja e Gabriela Prado Gomes, três estudantes da Faculdade de Direito da UERJ que atuam como colaboradoras voluntárias do LAEDH.
Além do senso comum: ensino de Sociologia e conhecimento científico
Authors: Fontes Barbara 
Published: January 2021

Chegamos à 26ª edição da Perspectiva Sociológica: A revista de professores de Sociologia no final desse difícil, triste e enlutado ano de 2020. Infelizmente, muitas das questões e incertezas trazidas no nosso editorial de julho ainda permanecem tanto no que se refere à educação quanto às políticas públicas para esse cenário pandêmico. Além de todos os desafios decorrentes do aumento da precariedade das condições de vida de muitos brasileiros e brasileiras, temos que lidar com angústias relacionadas ao medo do contágio pela COVID-19, à falta de um plano consistente de vacinação quando temos quase 200 mil mortos somente no Brasil e, não menos problemático, a um negacionismo em relação à ciência.Nesse cenário angustiante e delicado, publicações como a nossa e a Sociologia como disciplina escolar possuem grande importância para a desconstrução de determinados sensos comuns e para a compreensão das etapas e do rigor que caracterizam o conhecimento científico. Isso posto, esta edição, intitulada Além do senso comum: ensino de Sociologia e conhecimento científico, traz em suas diversas seções reflexões pautadas pelo conhecimento da tríade das Ciências Sociais sobre temas muito presentes no senso comum, como família, direitos humanos, violência e arte de rua.
The management of patient with osteoporosis in clinical practice
Authors: Degli Esposti Luca , Giacomini Elisa , Ghigi Alessandro , Perrone Valentina 
Published: January 2021

Osteoporosis is a systemic skeletal disorder characterized by increased bone fragility, which is associated with an enhanced fracture risk. The first fracture often represents indeed the clinical manifestation of this condition. In the present document we provided an overview of the economic and clinical impact of a not-adequate therapeutic appropriateness and suboptimal adherence to osteoporosis therapy, that are both widely reported in literature despite osteoporotic treatments have proved their efficacy in reducing fracture risk. Adequate treatment and adherence were reported to be associated with a lower risk of re-fracture and all-cause mortality. Moreover, healthcare costs in osteoporotic patients with previous fractures were significantly lower in those receiving osteoporosis treatment rather than among untreated patients. Nevertheless, these two key-factors are not improving over time. The measurement of indicators of adherence and therapeutic appropriateness allows to analyse the utilization profile of the drugs indicated for the treatment of osteoporosis and to evaluate the presence of possible deviation between the prescriptive behaviours observed in clinical practice and the recommendations reported in the guidelines. The periodic monitoring of such indicators together with prescribing audit activity could represent a useful tool for the optimization of osteoporosis management and to achieve a correct resource allocation. (Rheumatology)
New data of topographic anatomy of semilunar line (semilunar line by A. Spiegel)
Authors: Chernykh A. V. , Popova M. P. , Brigadirova V. Yu. 
Published: January 2021

Background. Today, a special place in surgical practice is the treatment of patients with spigelian hernias, which can be subcutaneous, interstitial, prepperitoneal, and make up 1% of all external hernias of the abdomen. The difficulty in diagnosing of spigelian hernias can lead to a serious complication – infringement of the hernia, which will require emergency surgical treatment. Therefore, the study of the typical, sexual and variant anatomy of the Spigelian line region is important in improving the diagnosis and treatment of such patients.The aim. To study the features of typical, sexual and variant anatomy of the Spigelian line region.Materials and methods. We examined 42 non-fixed corpses of persons of both sexes without signs of pathology of the anterior abdominal wall. Among them were 26 (54.2%) men and 22 (45.8%) women. At autopsy, we performed anatomical dissection of the semilunar line region. We measured the width of the aponeurotic stretch from the end of the transverse abdominal muscle fibers to the lateral edge of the rectus abdominis muscle at level corresponding to d. bicostarum, umbilical ring and d. bispinarum.Results. We identified four clusters corresponding to the variations in the shape of the semilunar line: tapering down (9.5%), uniform wide (19.0%), tapering up (28.6%) and wide in the middle (42.9%). We found that the semilunar line, tapering up, was significantly more often observed in women (83.3%), uniformly wide – in brachymorphic body type (75.0%), wide in the middle – in mesomorphic body type (66.07%), and the semilunar line, tapering down, was found only in men. Variants of the shape of the semilunar line, tapering up or down, were absent in persons of the brachymorphic body type. Semilunar line, tapering up, was found (without significant differences) in persons of the mesomorphic body type in 41.6%, in the persons of the brachymorphic body type – in 58.4%, and semilunar line, tapering down, was noted in persons with a dolichomorphic body type in 75.0%.Conclusion. New data may allow to predict the location, type of spigelian hernia, and also improve the diagnosis and treatment of spigelian hernia.
Exosomes of Human Umbilical Cord MSCs Protect Against Hypoxia/Reoxygenation-Induced Pyroptosis of Cardiomyocytes via the miRNA-100-5p/FOXO3/NLRP3 Pathway
Authors: Liang Chenyu , Liu Yang , Xu Huifeng , Huang Junling , Shen Yi , Chen Faxiu , Luo Ming 
Published: January 2021

BackgroundAcute myocardial infarction (AMI) is one of the leading causes of morbidity and death worldwide. Studies have indicated that microRNAs in mesenchymal stem cell (MSC)-derived exosomes are crucial for treating various diseases.MethodsHuman umbilical cord MSC (hucMSC)-derived exosomes (hucMSC-exo) were isolated and used to treat cardiomyocytes that underwent hypoxia/reoxygenation (H/R) injury. Bioluminescence assessment was used to study binding of miRNA to its targeting gene.ResultsWe found that H/R decreased the viability of AC16 cells, increased the expression of NLRP3, and activated caspase-1(p20) and GSDMD-N as well as release of IL-1β and IL-18, and such effects were abolished by administration of hucMSC-exo. Administration of exosomes from negative scramble miRNA (NC)-transfected hucMSCs blocked H/R-caused lactate dehydrogenase release, pyroptosis, and over-regulation of NLRP3 and activated caspase-1(p20) and GSDMD-N as well as release of IL-1β and IL-18. More importantly, in comparison to exsomes from NC-transfected hucMSCs, exsomes from miR-100-5p-overexpressing hucMSCs had more obvious effects, and those from miR-100-5p-inhibitor-transfected hucMSCs showed fewer effects. Functional study showed that miR-100-5p bound to the 3’-untranslated region (3’-UTR) of FOXO3 to suppress its transcription. Moreover, overexpression of FOXO3 abolished the protective effects of miR-100-5p.ConclusionEnriched miR-100-5p in hucMSC-exo suppressed FOXO3 expression to inhibit NLRP3 inflammasome activation and suppress cytokine release and, therefore, protected cardiomyocytes from H/R-induced pyroptosis and injury.
MicroRNA-505, Suppressed by Oncogenic Long Non-coding RNA LINC01448, Acts as a Novel Suppressor of Glycolysis and Tumor Progression Through Inhibiting HK2 Expression in Pancreatic Cancer
Authors: Xu Zhenglei , Zhang Dingguo , Zhang Zhuliang , Luo Weixiang , Shi Ruiyue , Yao Jun , Li Defeng , Wang Lisheng , Liao Bihong 
Published: January 2021

Background: MicroRNAs (miRNAs) and long non-coding RNAs (lncRNAs) play vital regulatory roles in pancreatic cancer (PC) initiation and progression. We aimed to explore the biological functions and underlying mechanisms of miR-505-3p (miR-505) in PC.Methods: We first screened miRNA expression profiles using microarray in PC tissues and normal tissues, and then studied the function and underlying mechanism of miR-505. Moreover, we evaluated the regulatory effect of lncRNA LINC01448 on miR-505.Results: We demonstrated miR-505 that was significantly downregulated in PC tissues. We further revealed that miR-505 significantly inhibited cell proliferation, invasion, sphere formation, glucose consumption, and lactate production by targeting HK2. In addition, overexpression of miR-505 led to tumor growth inhibition in vivo, demonstrating that it acts as a tumor suppressor in PC. LINC01448 was identified as an oncogenic lncRNA that could reduce miR-505 expression. Subsequent studies confirmed that LINC01448 enhanced cell proliferation, invasion, sphere formation, glucose consumption, and lactate production by regulating the miR-505/HK2 pathway.Conclusions: These findings demonstrated that miR-505, suppressed by LINC01448, could function as a key tumor suppressor by targeting HK2 in PC, elucidating an important role of the LINC01448/miR-505/HK2 pathway in regulating PC glycolysis and progression.
Identifying RBM47, HCK, CD53, TYROBP, and HAVCR2 as Hub Genes in Advanced Atherosclerotic Plaques by Network-Based Analysis and Validation
Authors: Liu Chiyu , Zhang Haifeng , Chen Yangxin , Wang Shaohua , Chen Zhiteng , Liu Zhaoyu , Wang Jingfeng 
Published: January 2021

Background:Atherosclerotic cardiovascular diseases accounted for a quarter of global deaths. Most of these fatal diseases like coronary atherosclerotic disease (CAD) and stroke occur in the advanced stage of atherosclerosis, during which candidate therapeutic targets have not been fully established. This study aims to identify hub genes and possible regulatory targets involved in treatment of advanced atherosclerotic plaques.Material/Methods:Microarray datasetGSE43292andGSE28829, both containing advanced atherosclerotic plaques group and early lesions group, were obtained from the Gene Expression Omnibus database. Weighted gene co-expression network analysis (WGCNA) was conducted to identify advanced plaque-related modules. Module conservation analysis was applied to assess the similarity of advanced plaque-related modules betweenGSE43292andGSE28829. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis of these modules were performed by Metascape. Differentially expressed genes (DEGs) were mapped into advanced plaque-related modules and module membership values of DEGs in each module were calculated to identify hub genes. Hub genes were further validated for expression in atherosclerotic samples, for distinguishing capacity of CAD and for potential functions in advanced atherosclerosis.Results:The lightgreen module (MElightgreen) inGSE43292and the brown module (MEbrown) inGSE28829were identified as advanced plaque-related modules. Conservation analysis of these two modules showed high similarity. GO and KEGG enrichment analysis revealed that genes in both MElightgreen and MEbrown were enriched in immune cell activation, secretory granules, cytokine activity, and immunoinflammatory signaling. RBM47, HCK, CD53, TYROBP, and HAVCR2 were identified as common hub genes, which were validated to be upregulated in advanced atherosclerotic plaques, to well distinguish CAD patients from non-CAD people and to regulate immune cell function-related mechanisms in advanced atherosclerosis.Conclusions:We have identified RBM47, HCK, CD53, TYROBP, and HAVCR2 as immune-responsive hub genes related to advanced plaques, which may provide potential intervention targets to treat advanced atherosclerotic plaques.
Contributions and Prognostic Values of N6-Methyladenosine RNA Methylation Regulators in Hepatocellular Carcinoma
Authors: Qi Li-Wen , Jia Jian-Hui , Jiang Chen-Hao , Hu Jian-Ming 
Published: January 2021

IntroductionThe methylation at position N6 of adenine is called N6-methyladenosine (m6A). This transcriptional RNA modification exerts a very active and important role in RNA metabolism and in other biological processes. However, the activities of m6A associated with malignant liver hepatocellular carcinoma (LIHC) are unknown and are worthy of study.Materials and MethodsUsing the data of University of California, Santa Cruz (UCSC), the expression of M6A methylation regulators in pan-cancer was evaluated as a screening approach to identify the association of M6A gene expression and 18 cancer types, with a specific focus on LIHC. LIHC datasets of The Cancer Genome Atlas (TCGA) were used to explore the expression of M6A methylation regulators and their clinical significance. Gene Ontology (GO) analysis and Gene Set Enrichment Analysis (GSEA) were used to explore the underlying mechanism based on the evaluation of aberrant expression of m6A methylation regulators.ResultsThe expression alterations of m6A-related genes varied across cancer types. In LIHC, we found that in univariate Cox regression analysis, up-regulated m6A modification regulators were associated with worse prognosis, except for ZC3H13. Kaplan–Meier survival curve analysis indicated that higher expression of methyltransferase-like protein 3 (METTL3) and YTH N6-methyladenosine RNA binding protein 1 (YTHDF1) genes related to the worse survival rate defined by disease-related survival (DSS), overall survival (OS), progression-free interval (PFI), and disease-free interval (DFI). Up-regulated m6A methylation regulator group (cluster2) obtained by consensus clustering was associated with poor prognosis. A six-gene prognostic signature established using the least absolute shrinkage and selection operator (LASSO) Cox regression algorithm performed better in the early (I + II; T1 + T2) stages than in the late (III + IV; T3 + T4) stages of LIHC. Using the gene signature, we constructed a risk score and found that it was an independent predictive factor for prognosis. Using GSEA, we identified processes involved in DNA damage repair and several biological processes associated with malignant tumors that were closely related to the high-risk group.ConclusionIn summary, our study identified several genes associated with m6A in LIHC, especially METTL3 and YTHDF1, and confirmed that a risk signature comprised of m6A-related genes was able to forecast prognosis.
Immunosenescence Study of T Cells: A Systematic Review
Authors: Rodriguez Ivon Johanna , Lalinde Ruiz Nicolás , Llano León Manuela , Martínez Enríquez Laura , Montilla Velásquez María del Pilar , Ortiz Aguirre Juan Pablo , Rodríguez Bohórquez Oscar Mauricio , Velandia Vargas Esteban Alejandro , Hernández Edgar Debray , Parra López Carlos Alberto 
Published: January 2021

BackgroundAging is accompanied by alterations in immune response which leads to increased susceptibility to infectious diseases, cancer, autoimmunity, and inflammatory disorders. This decline in immune function is termed as immunosenescence; however, the mechanisms are not fully elucidated. Experimental approaches of adaptive immunity, particularly for T cells, have been the main focus of immunosenescence research. This systematic review evaluates and discusses T cell markers implicated in immunosenescence.ObjectiveTo determine the best flow cytometry markers of circulating T cells associated with immunosenescence.MethodsWe systematically queried PubMed, MEDLINE, EBSCO, and BVS databases for original articles focused on two age groups of healthy humans: 18–44 (young adults) and &gt;60 (older adults) years. In accordance with the Cochrane methodology, we synthesized data through qualitative descriptions and quantitative random effects meta-analysis due to extensive heterogeneity.ResultsA total of 36 studies conducted in the last 20 years were included for the qualitative analysis and four out of these studies were used to perform the meta-analysis. A significant decrease in naïve T cell subset was observed in older adults compared to young adults. Primary markers used to identify senescent cells were loss of CD28 and increased expression of CD57 and KLRG1 in terminally-differentiated memory T cell subset in older adults. Moreover, we observed an increase in proinflammatory cytokines and decrease in telomere length in old adult T cells. It was not possible to perform quantitative synthesis on cell markers, cytokines, and telomere length because of the significant variations between the groups, which is attributed to differences in protocols and unreported measurements, thus generating a high risk of bias.ConclusionsHeterogeneity among studies in terms of data report, measurement techniques and high risk of bias were major impediments for performing a robust statistical analysis that could aid the identification of eligible flow cytometry markers of immunosenescence phenotype in T cells.
Case Report: Variable Pharmacokinetic Profile of Eculizumab in an aHUS Patient
Authors: Bouwmeester Romy N. , Ter Avest Mendy , Wijnsma Kioa L. , Duineveld Caroline , ter Heine Rob , Volokhina Elena B. , Van Den Heuvel Lambertus P. W. J. , Wetzels Jack F. M. , van de Kar Nicole C. A. J. 
Published: January 2021

BackgroundWith the introduction of eculizumab, a C5-inhibitor, morbidity and mortality improved significantly for patients with atypical hemolytic uremic syndrome (aHUS). In view of the high costs, actual needs of the drug, and increasing evidence in literature, aHUS patients can be treated according to a restrictive eculizumab regimen. We retrospectively analyzed the pharmacokinetic and dynamic parameters of eculizumab in one patient in time, emphasizing various factors which could be taken into account during tapering of treatment.Case PresentationA nowadays 18-year-old male with a severe, frequently relapsing form of atypical HUS due to a hybrid CFH/CFHR1 gene in combination with the homozygous factor H haplotype, required chronic plasma therapy (PT), including periods with plasma infusion, from the age of onset at 5 months until initiation of eculizumab at the age of 11 years. A mild but stable chronic kidney disease (CKD) and 9 years of disease remission enabled prolongation of eculizumab interval. At the age of 15 years, a sudden yet multifactorial progression of chronic kidney disease (CKD) was observed, without any signs of disease recurrence. However, an acquired glomerulocystic disease, a reduced left kidney function, and abnormal abdominal venous system of unknown etiology were found. In addition, after an aHUS relapse, an unexpected increase in intra-patient variability of eculizumab concentrations was seen. Retrospective pharmacokinetic analysis revealed a change in eculizumab clearance, associated with a simultaneous increase in proteinuria.ConclusionHigh intra-patient variability of eculizumab pharmacokinetics were observed over time, emphasizing the necessity for adequate and continuous therapeutic drug monitoring in aHUS patients. Eculizumab serum trough levels together with complement activation markers (CH50) should be frequently assessed, especially during tapering of drug therapy and/or changing clinical conditions in the patient. In addition, an increase in proteinuria could result in urinary eculizumab loss, indicating that urinary monitoring of eculizumab may be important in aHUS patients with an unexplained decline in serum concentrations.
Rehabilitation and Biomarkers of Stroke Recovery: Study Protocol for a Randomized Controlled Trial
Authors: Picelli Alessandro , Filippetti Mirko , Del Piccolo Lidia , Schena Federico , Chelazzi Leonardo , Della Libera Chiara , Donadelli Massimo , Donisi Valeria , Fabene Paolo Francesco , Fochi Stefania , Fonte Cristina , Gandolfi Marialuisa , Gomez-Lira Macarena , Locatelli Elena , Malerba Giovanni , Mariotto Sofia , Milanese Chiara , Patuzzo Cristina , Romanelli Maria Grazia , Sbarbati Andrea , Tamburin Stefano , Venturelli Massimo , Zamparo Paola , Carcereri de Prati Alessandra , Butturini Elena , Varalta Valentina , Smania Nicola 
Published: January 2021

Background: Stroke is a leading cause of disability. Nonetheless, the care pathway for stroke rehabilitation takes partially into account the needs of chronic patients. This is due in part to the lack of evidence about the mechanisms of recovery after stroke, together with the poor knowledge of related and influencing factors. Here we report on the study protocol “Rehabilitation and Biomarkers of Stroke Recovery,” which consists of 7 work-packages and mainly aim to investigate the effects of long-term neurorehabilitation on stroke patients and to define a related profile of (clinical-biological, imaging, neurophysiological, and genetic-molecular) biomarkers of long-term recovery after stroke. The work-package 1 will represent the main part of this protocol and aims to compare the long-term effects of intensive self-rehabilitation vs. usual (rehabilitation) care for stroke.Methods: We planned to include a total of 134 adult subacute stroke patients (no more than 3 months since onset) suffering from multidomain disability as a consequence of first-ever unilateral ischemic stroke. Eligible participants will be randomly assigned to one of the following groups: intensive self-rehabilitation (based on the principles of “Guided Self-Rehabilitation Contract”) vs. usual care (routine practice). Treatment will last 1 year, and patients will be evaluated every 3 months according to their clinical presentation. The following outcomes will be considered in the main work-package: Fugl-Meyer assessment, Cognitive Oxford Screen Barthel Index, structural and functional neuroimaging, cortical excitability, and motor and somatosensory evoked potentials.Discussion: This trial will deal with the effects of an intensive self-management rehabilitation protocol and a related set of biomarkers. It will also investigate the role of training intensity on long-term recovery after stroke. In addition, it will define a set of biomarkers related to post-stroke recovery and neurorehabilitation outcome in order to detect patients with greater potential and define long-term individualized rehabilitation programs.Clinical Trial Registration:www.ClinicalTrials.gov, identifier: NCT04323501.