bluuebunny/crossref_metadata_2025_split huggingface dataset:
OLIG2 maintenance is not essential for diffuse intrinsic pontine glioma cell line growth but regulates tumor phenotypes
Authors: Liao Yunfei , Luo Zaili , Deng Yaqi , Zhang Feng , Rao Rohit , Wang Jiajia , Xu Lingli , Kumar Shiva Senthil , Sengupta Satarupa , DeWire-Schottmiller Mariko , Berry Kalen , Garrett Matthew , Fouladi Maryam , Drissi Rachid , Lu Qing Richard 
Published: February 2021

AbstractBackgroundDiffuse intrinsic pontine glioma (DIPG) is a pediatric lethal high-grade brainstem glioma with no effective therapies. OLIG2 (oligodendrocyte transcription factor 2) was reported to be critical for the growth of a DIPG cell line CCHMC-DIPG-1. Surprisingly, we found that the CCHMC-DIPG-1 cells express little OLIG2 and exhibit a mesenchymal phenotype, which raised a question regarding the role of OLIG2 in the growth of DIPG cells.MethodsWe evaluated the function of OLIG2 in different DIPG cell lines through molecular and genetic approaches and performed transcriptomic and genomic landscape profiling including whole-genome bisulfite sequencing, RNA-seq, ATAC-seq, and ChIP-seq. shRNA-mediated knockdown and CRISPR-Cas9-mediated knockout approaches were utilized to assess OLIG2 functions in DIPG cell growth.ResultsWe found that DIPG cells are phenotypically heterogeneous and exhibit the characteristics of distinct malignant gliomas including proneural, classical, and mesenchymal subtypes. OLIG2 knockdown did not impact the growth of CCHMC-DIPG-1 cells, wherein OLIG2 is epigenetically silenced. Moreover, OLIG2 deletion did not substantially impair OLIG2-expressing proneural-like DIPG growth but led to an upregulation of HIPPO-YAP1 and epidermal growth factor receptor (EGFR) signaling and a tumor phenotype shift. Targeting HIPPO-YAP1 and EGFR signaling in OLIG2-deficient DIPG cells inhibited tumor cell growth.ConclusionsOur data indicate that OLIG2 is dispensable for DIPG growth but regulates the phenotypic switch of DIPG tumor cells. OLIG2 downregulation leads to deregulation of adaptive YAP1 and EGFR signaling. Targeting YAP1 and EGFR pathways inhibits the growth of OLIG2-deficient DIPG cells, pointing to a therapeutic potential by targeting adaptive signaling to treat DIPG tumors with nominal OLIG2 expression.
The impact of socioeconomic status (SES) on cognitive outcomes following radiotherapy for pediatric brain tumors: a prospective, longitudinal trial
Authors: Torres Victoria A , Ashford Jason M , Wright Evelyn , Xu Jiahui , Zhang Hui , Merchant Thomas E , Conklin Heather M 
Published: February 2021

Abstract

Background
Socioeconomic status (SES) is a determinant of cognitive and academic functioning among healthy and ill children; however, few pediatric oncology studies examine SES and long-term cognitive functioning. The current study systematically investigated SES as a predictor of cognitive outcomes among children treated for localized brain tumors (BT) with photon radiation therapy (RT).

Methods
248 children treated on a prospective, longitudinal, phase II trial of conformal RT (54-59.4 Gy) for ependymoma, low-grade glioma, or craniopharyngioma were monitored serially with cognitive assessments (intelligence quotient [IQ], reading, math, attention, adaptive function) for 10 years (2209 observations, median age at RT = 6.6 years, 48% male, 80% Caucasian). SES was derived from the Barratt Simplified Measure of Social Status, which incorporates parental occupation, education, and marital status.

Results
Overall, SES scores fell in the low range (Barratt median = 37). At pre-RT baseline, linear mixed models revealed significant associations between SES and IQ, reading, math, attention, and adaptive function, with higher SES associated with better performance (P &lt; .005). SES predicted change over time in IQ, reading, and math; higher SES was associated with less decline (P &lt; .001). Accounting for sex and age at RT, SES remained predictive of IQ, reading, and math. Analysis of variance revealed a greater relative contribution of SES than sex or age at RT to reading and math.

Conclusions
SES represents a novel predictor of cognitive performance before and after RT for pediatric BT. These findings have broad implications as high SES represents a protective factor. Developing interventions to mitigate the effects of low SES is warranted.
Transmission Routes of Severe Acute Respiratory Syndrome Coronavirus 2 Among Healthcare Workers of a French University Hospital in Paris, France
Authors: Contejean Adrien , Leporrier Jérémie , Canouï Etienne , Fourgeaud Jacques , Mariaggi Alice-Andrée , Alby-Laurent Fanny , Lafont Emmanuel , Beaudeau Lauren , Rouzaud Claire , Lecieux Fabienne , Greffet Agnès , L’Honneur Anne-Sophie , Tréluyer Jean-Marc , Lanternier Fanny , Casetta Anne , Frange Pierre , Leruez-Ville Marianne , Rozenberg Flore , Lortholary Olivier , Kernéis Solen 
Published: February 2021

Abstract
In this case-control study on 564 healthcare workers of a university hospital in Paris (France), contacts without protection with coronavirus disease 2019 (COVID-19) patients or with colleagues were associated with infection with severe acute respiratory syndrome coronavirus 2, whereas working in a COVID-dedicated unit and having children kept in childcare facilities were not.
Phytoplankton carbon and nitrogen biomass estimates are robust to volume measurement method and growth environment
Authors: Mcnair Heather , Hammond Courtney Nicole , Menden-Deuer Susanne 
Published: February 2021

Abstract
Phytoplankton biomass is routinely estimated using relationships between cell volume and carbon (C) and nitrogen (N) content that have been defined using diverse plankton that span orders of magnitude in size. Notably, volume has traditionally been estimated with geometric approximations of cell shape using cell dimensions from planar two-dimensional (2D) images, which requires assumptions about the third, depth dimension. Given advances in image processing, we examined how cell volumes determined from three-dimensional (3D), confocal images affected established relationships between phytoplankton cell volume and C and N content. Additionally, we determined that growth conditions could result in 30–40% variation in cellular N and C. 3D phytoplankton cell volume measurements were on average 15% greater than the geometric approximations from 2D images. Volume method variation was minimal compared to both intraspecific variation in volumes (~30%) and the 50-fold variation in elemental density among species. Consequently, C:vol and N:vol relationships were unaltered by volume measurement method and growth environment. Recent advances in instrumentation, including those for at sea and autonomous applications can be used to estimate plankton biomass directly. Going forward, we recommend instrumentation that permits species identification alongside size and shape characteristics for plankton biomass estimates.
Acceptance of a Covid-19 vaccine is associated with ability to detect fake news and health literacy
Authors: Montagni I , Ouazzani-Touhami K , Mebarki A , Texier N , Schück S , Tzourio C ,   the CONFINS group
Published: February 2021

ABSTRACT

Background
During the Covid-19 pandemic fake news has been circulating impacting on the general population’s opinion about a vaccine against the SARS-CoV-2. Health literacy measures the capacity of navigating health information.

Methods
We used data from a prospective national online cohort of 1647 participants. Descriptive statistics, Chi2 and ANOVA independence tests and two multivariable multinomial regression models were performed. Interactions between each variable were tested.

Results
Detection of fake news and health literacy scores were associated with intention to get vaccinated against SARS-CoV-2 (p &lt; 0.01). The risk of being “anti-vaccination” or “hesitant”, rather than “pro-vaccination”, was higher among individuals reporting bad detection of fake news, respectively OR = 1.93 (95%CI = [1.30;2.87]) and OR = 1.80 (95%CI = [1.29;2.52]). The risk of being in “hesitant”, rather than “pro-vaccination” was higher among individuals having a bad health literacy score (OR = 1.44; 95%CI = [1.04;2.00]). No interaction was found between detection of fake news and health literacy.

Conclusions
To promote acceptance of a vaccine against SARS-CoV-2, it is recommended to increase individuals’ ability to detect fake news and health literacy through education and communication programs.
‘And with your spirit’: a holistic support for healthcare workers in the midst of COVID-19 pandemic
Authors: Cordero Dalmacito A 
Published: February 2021

Abstract
Public health is characterized by social justice where it has the obligation to ‘give what is due’ and address the needs of the entire community. Recent correspondence calls for the physical, financial and mental support of the government to our ‘distressed’ healthcare workers. However, this seems to insufficient since the spiritual well-being is set aside and Filipinos are known to be religious people by their very nature. I therefore propose and highlight the use of a holistic approach which emphasizes the needed support which caters the faith dimension of our frontliners. The proposals include the availability of human resource and religious practices in both public and private hospitals where the healthcare workers do their battle relentlessly.
Compassion fatigue of funeral directors during and after the first wave of COVID-19
Authors: Van Overmeire Roel , Van Keer Rose-Lima , Cocquyt Marie , Bilsen Johan 
Published: February 2021

Abstract

Background
Compassion fatigue has not been studied among funeral directors. Yet, funeral directors have been exposed to the same risks for compassion fatigue as other caregivers during the coronavirus disease 2019 (COVID-19) pandemic.

Methods
An online survey was spread two times to 287 employees of funeral home DELA, in Belgium. Once during the height of the first wave of COVID-19 in Belgium, and a second time at the end of the first wave. The professional quality of life-scale 5 (PROQOL-5) was used to measure compassion fatigue, which includes burnout, compassion satisfaction and secondary trauma. Non-parametric tests were performed.

Results
In total, 104 participants answered the first survey, and 107 the second. Burnout increases from survey 1 to survey 2 (P &lt; 0.001), while compassion satisfaction (P = 0.011) and secondary trauma decrease (P &lt; 0.001). In survey 1, only age (P = 0.007) and gender (P = 0.040) were found to be significantly associated with secondary trauma. In survey 2, having more work experience is associated with having a higher burnout (P = 0.008) and secondary trauma (P = 0.001) score. Neither for burnout (P &lt; 0.001), nor for secondary trauma (P &lt; 0.001) are there any respondents in the highest category.

Conclusions
Although overall funeral directors do not have acute problems with compassion fatigue, burnout scores increase significantly after the first wave.
‘We are not the virus’: stigmatization and discrimination against frontline health workers
Authors: Corpuz Jeff Clyde G 
Published: February 2021

Abstract
During the current COVID-19 pandemic, several forms of stigma and discrimination have been reported worldwide mainly from toward Asian descent, those with international travel history, and frontline health workers. In response to the situation of stigmatization and discrimination against frontline healthcare workers, the author proposes several ways on how the public and the Philippine government might create safety guidelines by revisiting the international and local laws. Thus, there is an urgent need to end the stigma, discrimination and other forms of inhumane treatment against our frontline health workers. They are not the virus.
The Declining Significance of Race in Criminal Sentencing: Evidence from US Federal Courts
Authors: Light Michael T 
Published: February 2021

AbstractRacial inequality in sentencing has decreased substantially over the last decade. In 2009, the average sentencing difference between black and white defendants in federal court was nearly 3 yrs. By 2018, this difference was less than 6 mos. Among drug offenders over this same period, the black–white gap went from 47 mos. down to zero. Yet, despite the fact that racial inequality in the legal system remains at the fore of sociological discourse, these developments remain conspicuously under-evaluated and the underlying processes driving these changes remain unknown. This article fills this gap by applying longitudinal decomposition methods to US District Court data between 2009 and 2018. Three notable findings emerge. First, the declining racial gap was driven, in equal parts, by decreasing black sentences and increasing white sentences. Second, black and white sentences became more equal almost entirely due to changes in observable case characteristics and not due to changes in the treatment of offenders. Third, shifts in the prosecutorial use of mandatory minimums played a critical role in decreasing black–white sentencing inequality.
A standardized terminology of spines in the order Siluriformes (Actinopterygii: Ostariophysi)
Authors: Ballen Gustavo A , De Pinna Mario C C 
Published: February 2021

AbstractA standardized terminology for the anatomy of pectoral- and dorsal-fin spines in the order Siluriformes is proposed based on an extensive literature review and direct examination of representatives of the order. The adult anatomy of the spines is described in detail. Terminology of various spine parts are reviewed and standardized, each term provided with a synonymic list organizing previous usage. Most of the structures treated have been recorded and named in the literature, but some are herein named for the first time. A quantitative approach is proposed for orienting decisions on name usage, aiming at minimizing differences between the terminology proposed and the vast amount of pre-existing literature, herein called the cost function. It is expected that this system will aid efforts in organizing the chaotic anatomical nomenclature of the appendicular skeleton in Siluriformes, and provide a solid basis for advances in comparative anatomy and nomenclature. The proposed terminology system has potential application on a number of fields that utilize information from catfish spines, ranging from taxonomy to phylogenetic systematics to paleontology and archaeology.
Residues R192 and K225 in RNA-Binding Pocket of Tobacco Vein Banding Mosaic Virus CP Control Virus Cell-to-Cell Movement and Replication
Authors: Yan Zhi-Yong , Xu Xiao-Jie , Fang Le , Cheng De-Jie , Tian Yan-Ping , Geng Chao , Li Xiang-Dong , Valkonen Jari P. T. 
Published: February 2021

Potyviruses move to neighboring cells in the form of virus particles or a coat protein (CP)-containing ribonucleoprotein complex. However, the precise roles of RNA-binding residues in potyviral CP in viral cell-to-cell movement remain to be elucidated. In this study, we predicted the three-dimensional model of tobacco vein banding mosaic virus (TVBMV)-encoded CP and found nine residues presumably located in the CP RNA-binding pocket. Substitutions of the two basic residues at positions 192 and 225 (R192 and K225) with either alanine, cysteine, or glutamic acid abolished TVBMV cell-to-cell and systemic movement in Nicotiana benthamiana plants. These substitutions also reduced the replication of the mutant viruses. Results from the electrophoretic mobility shift assay showed that the RNA-binding activity of mutant CPs derived from R192 or K225 substitutions was significantly lower than that of wild-type CP. Analysis of purified virus particles showed that mutant viruses with R192 or K225 substitutions formed RNA-free virus-like particles. Mutations of R192 and K225 did not change the CP plasmodesmata localization. The wild-type TVBMV CP could rescue the deficient cell-to-cell movement of mutant viruses. Moreover, deletion of any of the other seven residues also abolished TVBMV cell-to-cell movement and reduced the CP RNA-binding activity. The corresponding nine residues in watermelon mosaic virus CP were also found to play essential roles in virus cell-to-cell movement. In conclusion, residues R192 and K225 in the CP RNA-binding pocket are critical for viral RNA binding and affect both virus replication and cell-to-cell movement. [Formula: see text] Copyright © 2021 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license .
High proportion of genome-wide homology and increased basal pvcrt levels in Plasmodium vivax late recurrences: a chloroquine therapeutic efficacy study
Authors: Rovira-Vallbona Eduard , Van Hong Nguyen , Kattenberg Johanna H. , Huan Ro Mah , Binh Nguyen Thi Huong , Ngọc Nguyen Thi Hong , Guetens Pieter , Hieu Nguyen Luong , Thị Thu Hien Nguyen , Sang Vu Thi , Long Nguyen Duc , Sauve Erin , Duong Tran Thanh , Xa Nguyen Xuan , Erhart Annette , Rosanas-Urgell Anna 
Published: February 2021

ABSTRACTChloroquine (CQ) is the first-line treatment for Plasmodium vivax malaria in most endemic countries. Monitoring P.vivax CQ resistance (CQR) is critical but remains challenged by the difficulty to distinguish real treatment failure from reinfection or liver relapse. Therapeutic efficacy of CQ against uncomplicated P.vivax malaria was evaluated in Gia Lai province, Vietnam. Sixty-seven patients were enrolled and followed-up for 42 days using microscopy and (RT)qPCR. Adequate clinical and parasitological response (ACPR) was 100% (66/66) on Day 28, but 75.4% (49/65) on Day 42. Eighteen recurrences (27.7%) were detected with a median time-to-recurrence of 42 days (IQR 35, 42) and blood CQ concentration <100ng/ml. Parasite genotyping by microsatellites, SNP-barcoding and whole-genome sequencing (WGS) identified a majority of homologous recurrences, with 80% (8/10) showing >98% identity-by-descent to paired Day 0 samples. Primary infections leading to recurrence occurred in younger individuals (median age for ACPR=25 years [IQR 20, 28]; recurrences=18 [16, 21]; p=0.002), had a longer parasite clearance time (PCT for ACPR=47.5h [IQR 36.2, 59.8]; recurrences=54.2h [48.4, 62.0]; p=0.035) and higher pvcrt gene expression (median relative expression ratio for ACPR=0.09 [IQR 0.05, 0.22]; recurrences=0.20 [0.15, 0.56]; p=0.002), but there was no difference in ex vivo CQ sensitivity. This study shows that CQ remained largely efficacious to treat P.vivax in Gia Lai, i.e. recurrences occurred late (>Day 28) and in the presence of low blood CQ concentrations. However, the combination of WGS and gene expression analysis (pvcrt) with clinical data (PCT) allowed to identify potential emergence of low-grade CQR that should be closely monitored.
A Molecular network approach reveals shared cellular and molecular signatures between chronic fatigue syndrome and other fatiguing illnesses
Authors: Comella Phillip H. , Gonzalez-Kozlova Edgar , Kosoy Roman , Charney Alexander W. , Peradejordi Irene Font , Chandrasekar Shreya , Tyler Scott R. , Wang Wenhui , Losic Bojan , Zhu Jun , Hoffman Gabriel E. , Kim-Schulze Seunghee , Qi Jingjing , Patel Manishkumar , Kasarskis Andrew , Suarez-Farinas Mayte , Gümüş Zeynep H. , Argmann Carmen , Merad Miriam , Becker Christian , Beckmann Noam D. , Schadt Eric E. 
Published: February 2021

IntroThe molecular mechanisms of chronic fatigue syndrome (CFS, or Myalgic encephalomyelitis), a disease defined by extreme, long-term fatigue, remain largely uncharacterized, and presently no molecular diagnostic test and no specific treatments exist to diagnose and treat CFS patients. While CFS has historically had an estimated prevalence of 0.1-0.5% [1], concerns of a “long hauler” version of Coronavirus disease 2019 (COVID-19) that symptomatically overlaps CFS to a significant degree(Supplemental Table-1)and appears to occur in 10% of COVID-19 patients[2], has raised concerns of a larger spike in CFS [3]. Here, we established molecular signatures of CFS and a corresponding network-based disease context from RNA-sequencing data generated on whole blood and FACs sorted specific peripheral blood mononuclear cells (PBMCs) isolated from CFS cases and non-CFS controls. The immune cell type specific molecular signatures of CFS we identified, overlapped molecular signatures from other fatiguing illnesses, demonstrating a common molecular etiology. Further, after constructing a probabilistic causal model of the CFS gene expression data, we identified master regulator genes modulating network states associated with CFS, suggesting potential therapeutic targets for CFS.
COVID-19 risk perceptions of social interaction and essential activities and inequity in the United States: Results from a nationally representative survey
Authors: Erchick Daniel J. , Zapf Alexander J. , Baral Prativa , Edwards Jeffrey , Mehta Shruti H. , Solomon Sunil S. , Gibson Dustin G. , Agarwal Smisha , Labrique Alain B. 
Published: February 2021

ABSTRACTIntroductionSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2) related diagnoses, hospitalizations, and deaths have disproportionately affected disadvantaged communities across the United States. Few studies have sought to understand how risk perceptions related to social interaction and essential activities during the COVID-19 pandemic vary by sociodemographic factors, information that could inform targeted interventions to reduce inequities in access to care and information.MethodsWe conducted a nationally representative online survey of 1,592 adults in the United States to understand risk perceptions related to transmission of COVID-19 for various social and essential activities. We assessed relationships for each activity, after weighting to adjust for the survey design, using bivariate comparisons and multivariable logistic regression modeling, between responses of safe and unsafe, and participant characteristics, including age, gender, race, education, income, and political affiliation.ResultsHalf of participants were younger than 45 years (n=844, 53.0%), female (n=800, 50.3%), and White/Caucasian (n=685, 43.0%), Black/African American (n=410, 25.8%), or Hispanic/Latino (n=382, 24.0%). Risk perceptions of unsafe for 13 activities ranged from 29.2% to 73.5%. Large gatherings, indoor dining, and visits with elderly relatives had the highest proportion of unsafe responses (>58%) while activities outdoor, visiting the doctor or dentist, and going to the grocery store had the lowest (<36%). Older respondents were more likely to view social gatherings and indoor activities as unsafe, yet more likely to view activities such as going to the grocery store, participating in outdoor activities, visiting elderly relatives, and visiting the doctor or emergency room as safe. Compared to White/Caucasian respondents, Black/African American and Hispanic/Latino respondents were more likely to view activities such as dining and visiting friends outdoor as unsafe. Generally, men vs. women, Republicans vs. Democrats and independents, and individuals with higher vs. lower income were more likely to view activities as safe.ConclusionsThese findings suggest the importance of sociodemographic differences in risk perception, health behaviors, and access to information and health care when implementing efforts to control the COVID-19 pandemic. Further research should address how evidence-based interventions can be tailored considering these differences with a goal of increased health equity in the pandemic response.