bluuebunny/crossref_metadata_2025_split huggingface dataset:
Characterization of a murine model of endothelial dysfunction induced by chronic intraperitoneal administration of angiotensin II
Authors: Trejo-Moreno Celeste , Jiménez-Ferrer Enrique , Castro-Martínez Gabriela , Méndez-Martínez Marisol , Santana María Angélica , Arrellín-Rosas Gerardo , Pedraza-Chaverri José , Medina-Campos Omar Noel , Hernández-Téllez Beatriz , Ramírez-Pliego Oscar , Herrera-Ruiz Maribel , Cervantes-Torres Jacquelynne , Alvarado-Ojeda Zimri Aziel , Costet-Mejía Alejandro , Fragoso Gladis , Rosas-Salgado Gabriela 
Published: October 2021

AbstractEndothelial dysfunction (ED) is a key factor for the development of cardiovascular diseases. Due to its chronic, life-threatening nature, ED only can be studied experimentally in animal models. Therefore, this work was aimed to characterize a murine model of ED induced by a daily intraperitoneal administration of angiotensin II (AGII) for 10 weeks. Oxidative stress, inflammation, vascular remodeling, hypertension, and damage to various target organs were evaluated in treated animals. The results indicated that a chronic intraperitoneal administration of AGII increases the production of systemic soluble VCAM, ROS and ICAM-1 expression, and the production of TNFα, IL1β, IL17A, IL4, TGFβ, and IL10 in the kidney, as well as blood pressure levels; it also promotes vascular remodeling and induces non-alcoholic fatty liver disease, glomerulosclerosis, and proliferative retinopathy. Therefore, the model herein proposed can be a representative model for ED; additionally, it is easy to implement, safe, rapid, and inexpensive.
Microbial diversity in Mediterranean sponges as revealed by metataxonomic analysis
Authors: Ruocco Nadia , Esposito Roberta , Zagami Giacomo , Bertolino Marco , De Matteo Sergio , Sonnessa Michele , Andreani Federico , Crispi Stefania , Zupo Valerio , Costantini Maria 
Published: October 2021

AbstractAlthough the Mediterranean Sea covers approximately a 0.7% of the world’s ocean area, it represents a major reservoir of marine and coastal biodiversity. Among marine organisms, sponges (Porifera) are a key component of the deep-sea benthos, widely recognized as the dominant taxon in terms of species richness, spatial coverage, and biomass. Sponges are evolutionarily ancient, sessile filter-feeders that harbor a largely diverse microbial community within their internal mesohyl matrix. In the present work, we firstly aimed at exploring the biodiversity of marine sponges from four different areas of the Mediterranean: Faro Lake in Sicily and “Porto Paone”, “Secca delle fumose”, “Punta San Pancrazio” in the Gulf of Naples. Eight sponge species were collected from these sites and identified by morphological analysis and amplification of several conserved molecular markers (18S and 28S RNA ribosomal genes, mitochondrial cytochrome oxidase subunit 1 and internal transcribed spacer). In order to analyze the bacterial diversity of symbiotic communities among these different sampling sites, we also performed a metataxonomic analysis through an Illumina MiSeq platform, identifying more than 1500 bacterial taxa. Amplicon Sequence Variants (ASVs) analysis revealed a great variability of the host-specific microbial communities. Our data highlight the occurrence of dominant and locally enriched microbes in the Mediterranean, together with the biotechnological potential of these sponges and their associated bacteria as sources of bioactive natural compounds.
Acidic fluids in the Earth’s lower crust
Authors: Samuel Vinod O. , Santosh M. , Jang Yirang , Kwon Sanghoon 
Published: October 2021

AbstractFluid flux through Earth’s surface and its interior causes geochemical cycling of elements in the Earth. Quantification of such process needs accurate knowledge about the composition and properties of the fluids. Knowledge about the fluids in Earth’s interior is scarce due to limitations in both experimental methods and thermodynamic modeling in high/ultrahigh pressure–temperature conditions. In this study, we present halogen (Cl, F) measurements in apatite grains from the mafic (metagabbro), and felsic (two-pyroxene granulite, charnockite, hornblende-biotite gneiss) rocks preserved in the Nilgiri Block, southern India. Previous experiments show that it is difficult to incorporate Cl in apatite compared to F at high pressure and temperature conditions. Based on regional trends in Cl and F content in apatite (with highest Cl content 2.95 wt%), we suggest the presence of acidic C–O–H fluids in the lower crust (~20–40 km deep) during the high-grade metamorphism of these rocks. These fluids are capable of causing extreme chemical alterations of minerals, especially refractory ones. They also have significant potential for mass transfer, causing extensive geochemical variations on a regional scale and altering the chemical and isotope records of rocks formed in the early Earth. Our findings have important relevance in understanding speciation triggered by acidic fluids in the lower crust, as well as the role of fluids in deep Earth processes.
Experimental study on the explosion characteristics of hydrogen-methane premixed gas in complex pipe networks
Authors: Jia Jinzhang , Chen Yinuo , Che Guangbo , Zhu Jinchao , Wang Fengxiao , Jia Peng 
Published: October 2021

AbstractTo explore the overpressure evolution laws and flame propagation characteristics in complex pipe networks after the addition of hydrogen to methane, we experimentally studied the explosive pressure wave and flame wave propagation laws for three different premixed gas mixtures with hydrogen-methane concentrations of 0, 10% and 20% when the equivalence ratio was 1. Experimental results indicate that the maximum explosion overpressure of the premixed gas increases with increasing distance from the explosion source, and it shows a gradually decreasing trend. In the complex pipe network, an overpressure zone is formed in the B–E–H and D–E sections of the network. The flame temperature is superimposed with the superimposition of the pressure, showing a trend of first increasing, then decreasing, then increasing, and finally decreasing in the complex pipe network. The flame arrival time increases with increasing distance, and the maximum flame speed shows a decreasing trend. The peak overpressure and maximum flame velocity of the premixed gas under a hydrogen volume fraction of 20% are 1.266 MPa and 168 m/s. The experimental research results could provide important theoretical guidelines for the prevention and control of fuel gas explosions in urban pipe networks.
CD157 signaling promotes survival of acute myeloid leukemia cells and modulates sensitivity to cytarabine through regulation of anti-apoptotic Mcl-1
Authors: Yakymiv Yuliya , Augeri Stefania , Bracci Cristiano , Marchisio Sara , Aydin Semra , D’Ardia Stefano , Massaia Massimo , Ferrero Enza , Ortolan Erika , Funaro Ada 
Published: October 2021

AbstractCD157/BST-1 (a member of the ADP-ribosyl cyclase family) is expressed at variable levels in 97% of patients with acute myeloid leukemia (AML), and is currently under investigation as a target for antibody-based immunotherapy. We used peripheral blood and bone marrow samples from patients with AML to analyse the impact of CD157-directed antibodies in AML survival and in response to cytarabine (AraC) ex vivo. The study was extended to the U937, THP1 and OCI-AML3 AML cell lines of which we engineered CD157-low versions by shRNA knockdown. CD157-targeting antibodies enhanced survival, decreased apoptosis and reduced AraC toxicity in AML blasts and cell lines. CD157 signaling activated the PI3K/AKT/mTOR and MAPK/ERK pathways and increased expression of Mcl-1 and Bcl-XL anti-apoptotic proteins, while decreasing expression of Bax pro-apoptotic protein, thus preventing Caspase-3 activation. The primary CD157-mediated anti-apoptotic mechanism was Bak sequestration by Mcl-1. Indeed, the Mcl-1-specific inhibitor S63845 restored apoptosis by disrupting the interaction of Mcl-1 with Bim and Bak and significantly increased AraC toxicity in CD157-high but not in CD157-low AML cells. This study provides a new role for CD157 in AML cell survival, and indicates a potential role of CD157 as a predictive marker of response to therapies exploiting Mcl-1 pharmacological inhibition.
Selective and predicable amine conjugation sites by kinetic characterization under excess reagents
Authors: Huang Wei-Chun , Huang Li-Juan , Hsu Liang-Sheng , Huang Shih-Ting , Lo Wen-Ting , Wang Tzu-Fan , Sun Wei-Ting , Wei Win-Yin , Lee Ying-Shuan , Chuang Shih-Hsien , Lee Chao-Pin , Chou Ho-Hsuan , Chen Shu-Hui 
Published: October 2021

AbstractThe site selectivity for lysine conjugation on a native protein is difficult to control and characterize. Here, we applied mass spectrometry to examine the conjugation kinetics of Trastuzumab-IgG (Her-IgG) and α-lactalbumin under excess linker concentration ([L]0) based on the modified Michaelis–Menten equation, in which the initial rate constant per amine (kNH2 = Vmax/NH2/KM) was determined by the maximum reaction rate (Vmax/NH2) under saturated accessible sites and initial amine–linker affinity (1/KM). Reductive amination (RA) displayed 3–4 times greater Vmax/NH2 and a different panel of conjugation sites than that observed for N-hydroxysuccinimide ester (NHS) chemistry using the same length of polyethylene glycol (PEG) linkers. Moreover, faster conversion power rendered RA site selectivity among accessible amine groups and a greater tunable range of linker/protein ratio for aldehyde-linkers compared to those of the same length of NHS-linkers. Single conjugation with high yield or poly-conjugations with site homogeneity was demonstrated by controlling [L]0 or gradual addition to minimize the [L]0/KM ratio. Formaldehyde, the shortest aldehyde-linker with the greatest 1/KM, exhibited the highest selectivity and was shown to be a suitable probe to predict conjugation profile of aldehyde-linkers. Four linkers on the few probe-predicted hot spots were elucidated by kinetically controlled RA with conserved drug efficacy when conjugated with the payload. This study provides insights into controlling factors for homogenous and predictable amine bioconjugation.
Pathophysiological and diagnostic importance of fatty acid-binding protein 1 in heart failure with preserved ejection fraction
Authors: Harada Tomonari , Araki Takeshi , Sunaga Hiroaki , Kagami Kazuki , Yoshida Kuniko , Kato Toshimitsu , Kawakami Ryo , Tomono Junichi , Wada Naoki , Iso Tatsuya , Kurabayashi Masahiko , Obokata Masaru 
Published: October 2021

AbstractElevated intracardiac pressure at rest and/or exercise is a fundamental abnormality in heart failure with preserved ejection fraction (HFpEF). Fatty acid-binding protein 1 (FABP1) is proposed to be a sensitive biomarker for liver injury. We sought to determine whether FABP1 at rest would be elevated in HFpEF and would correlate with echocardiographic markers of intracardiac pressures at rest and during exercise. In this prospective study, subjects with HFpEF (n = 22) and control subjects without HF (n = 23) underwent resting FABP1 measurements and supine bicycle exercise echocardiography. Although levels of conventional hepatic enzymes were similar between groups, FABP1 levels were elevated in HFpEF compared to controls (45 [25–68] vs. 18 [14–24] ng/mL, p = 0.0008). FABP1 levels were correlated with radiographic and blood-based markers of congestion, hemodynamic derangements during peak exercise (E/e’, r = 0.50; right atrial pressure, r = 0.35; pulmonary artery systolic pressure, r = 0.46), reduced exercise cardiac output (r = − 0.49), and poor exercise workload achieved (r = − 0.40, all p < 0.05). FABP1 distinguished HFpEF from controls with an area under the curve of 0.79 (p = 0.003) and had an incremental diagnostic value over the H2FPEF score (p = 0.007). In conclusion, FABP1 could be a novel hepatic biomarker that associates with hemodynamic derangements, reduced cardiac output, and poor exercise capacity in HFpEF.
Polymerization kinetics of experimental resin composites functionalized with conventional (45S5) and a customized low-sodium fluoride-containing bioactive glass
Authors: Par Matej , Prskalo Katica , Tauböck Tobias T. , Skenderovic Hrvoje , Attin Thomas , Tarle Zrinka 
Published: October 2021

AbstractThis study aimed to investigate polymerization kinetics and curing light transmittance of two series of experimental dental resin composites filled with 0–40 wt% of either 45S5 bioactive glass (BG) or a customized low-Na F-containing BG. Polymerization kinetics in 0.1-mm and 2-mm thick layers were investigated through real-time degree of conversion measurements using a Fourier transform infrared (FTIR) spectrometer. FTIR spectra were continuously collected at a rate of 2 s−1 during light-curing (1340 mW/cm2). Light transmittance through 2-mm thick composite specimens was measured using a UV–Vis spectrometer at a rate of 20 s−1. Unlike BG 45S5, which led to a dose-dependent reduction in the rate and extent of polymerization, the customized low-Na F-containing BG showed a negligible influence on polymerization. The reduction in light transmittance of experimental composites due to the addition of the low-Na F-containing BG did not translate into impaired polymerization kinetics. Additionally, the comparison of polymerization kinetics between 0.1-mm and 2-mm thick layers revealed that polymerization inhibition identified for BG 45S5 was not mediated by an impaired light transmittance, indicating a direct effect of BG 45S5 on polymerization reaction. A customized low-Na F-containing BG showed favourable behaviour for being used as a functional filler in light-curing dental resin composites.
Exposure to respirable dust among workers fabricating aluminium trihydroxide-containing synthetic countertops
Authors: Vinnikov Denis , Blanc Paul D. , Raushanova Aizhan , Beisbekova Arailym , Abraham Jerrold L. , Zlobina Yelena 
Published: October 2021

AbstractThe aim of this study is to characterize personal exposure of workers to respirable particulate matter (PM) generated in cutting and other fabrication activities when fabricating acryl polymer/aluminium trihydroxide synthetic countertops. We collected 29 personal full-day samples of respirable PM from three workers in a small private workshop. We tested differences between- and within-worker variances of mass concentrations using the Kruskall-Wallis test. We used segmented regression to test the means and medians 15-min interval concentrations changes over time and to identify a breakpoint. Respirable PM concentrations ranged nearly 100-fold, from 0.280 to 25.4 mg/m3 with a median of 2.0 mg/m3 (1-min concentrations from 13,920 data points). There were no statistical difference in daily median or geometric mean concentrations among workers, whereas the concentrations were significantly higher on days with three versus two workers present. The 15-min median concentrations (n = 974 measures) increased until 2.35 h (beta 0.177; p < 0.05), representing a 0.70 mg increase in exposure per hour. This was followed by a plateau in concentrations. The high levels of respirable PM we observed among workers fabricating aluminium trihydroxide-containing synthetic countertops highlight an unmet early prevention need.
Quantum Fisher information of two atoms with dipole–dipole interaction under the environment of phase noise lasers
Authors: Chen Yu , Long Zheng-wen , He Zhi , Ji Shen-tong 
Published: October 2021

AbstractWe investigate the parameter estimation problems of two-atom system driven by the phase noise lasers (PNLs) environment. And we give a general method of numeric solution to handle the problems of atom system under the PNLs environment. The calculation results of this method on Quantum Fisher Information (QFI) are consistent with our former results. Moreover, we consider the dipole–dipole (d–d) interaction between the atoms under PNLs environment with the collective decay, and the results show that larger d–d interaction and smaller collective decay rate lead to larger QFI of the two-atom system. So the collective decay will destroy the QFI while the d–d interaction will preserve the QFI, these results can be used to protect the QFI of two-atom system driven by the PNLs environment.
GD2 CAR T cells against human glioblastoma
Authors: Prapa Malvina , Chiavelli Chiara , Golinelli Giulia , Grisendi Giulia , Bestagno Marco , Di Tinco Rosanna , Dall’Ora Massimiliano , Neri Giovanni , Candini Olivia , Spano Carlotta , Petrachi Tiziana , Bertoni Laura , Carnevale Gianluca , Pugliese Giuseppe , Depenni Roberta , Feletti Alberto , Iaccarino Corrado , Pavesi Giacomo , Dominici Massimo 
Published: October 2021

AbstractGlioblastoma is the most malignant primary brain tumor and is still in need of effective medical treatment. We isolated patient-derived glioblastoma cells showing high GD2 antigen expression representing a potential target for CAR T strategy. Data highlighted a robust GD2 CAR antitumor potential in 2D and 3D glioblastoma models associated with a significant and CAR T-restricted increase of selected cytokines. Interestingly, immunosuppressant TGF β1, expressed in all co-cultures, did not influence antitumor activity. The orthotopic NOD/SCID models using primary glioblastoma cells reproduced human histopathological features. Considering still-conflicting data on the delivery route for targeting brain tumors, we compared intracerebral versus intravenous CAR T injections. We report that the intracerebral route significantly increased the length of survival time in a dose-dependent manner, without any side effects. Collectively, the proposed anti-GD2 CAR can counteract human glioblastoma potentially opening a new therapeutic option for a still incurable cancer.
Harnessing the Electronic Health Record to Actively Support Providers with Guideline-Directed Telemetry Use
Authors: Narayanan Maya , Starks Helene , Tanenbaum Eric , Robinson Ellen , Sutton Paul R. , Schleyer Anneliese M. 
Published: October 2021

Abstract
Background Overuse of cardiac telemetry monitoring (telemetry) can lead to alarm fatigue, discomfort for patients, and unnecessary medical costs. Currently there are evidence-based recommendations describing appropriate telemetry use, but many providers are unaware of these guidelines.
Objectives At our multihospital health system, our goal was to support providers in ordering telemetry on acute care in accordance with evidence-based guidelines and discontinuing telemetry when it was no longer medically indicated.
Methods We implemented a multipronged electronic health record (EHR) intervention at two academic medical centers, including: (1) an order set requiring providers to choose an indication for telemetry with a recommended duration based on American Heart Association guidelines; (2) an EHR-generated reminder page to the primary provider recommending telemetry discontinuation once the guideline-recommended duration for telemetry is exceeded; and (3) documentation of telemetry interpretation by telemetry technicians in the notes section of the EHR. To determine the impact of the intervention, we compared number of telemetry orders actively discontinued prior to discharge and telemetry duration 1 year pre- to 1 year post-intervention on acute care medicine services. We evaluated sustainability at years 2 and 3.
Results Implementation of the EHR initiative resulted in a statistically significant increase in active discontinuation of telemetry orders prior to discharge: 15% (63.4–78.7%) at one site and 13% at the other (64.1–77.4%) with greater improvements on resident teams. Fewer acute care medicine telemetry orders were placed on medicine services across the system (1,503–1,305) despite an increase in admissions and the average duration of telemetry decreased at both sites (62 to 47 hours, p < 0.001 and 73 to 60, p < 0.001, respectively). Improvements were sustained 2 and 3 years after intervention.
Conclusion Our study showed that a low-cost, multipart, EHR-based intervention with active provider engagement and no additional education can decrease telemetry usage on acute care medicine services.
Uterine Isthmocele—A Frequently Overlooked Complication of Cesarean Sections
Authors: Rupa R. , Kushvaha Suchana , Venkatesh K. 
Published: October 2021

AbstractUterine isthmocele or uterine niche is a late complication of cesarean deliveries and causes chronic pelvic pain, menorrhagia or postmenstrual spotting, and infertility. As the number of cesarean sections are constantly increasing, it is important to be aware of this entity so as to make an early diagnosis. This would enable the clinicians to manage these patients efficiently. We present three patients of uterine isthmocele who were evaluated and managed at our institution.
Prognostic Significance of EMVI in Rectal Cancer in a Tertiary Cancer Hospital in India
Authors: Pangarkar Sayali Y. , Baheti Akshay D. , Mistry Kunal A. , Choudhari Amit J. , Patil Vasundhara R. , Ahuja Ankita , Katdare Aparna N. , Patil Akshay B. , Ostwal Vikas S. , Ramadwar Mukta R. , Engineer Reena Z. , Saklani Avanish P. 
Published: October 2021

Abstract
Background Presence of extramural venous invasion (EMVI) is a poor prognostic factor for rectal cancer as per literature. However, India-specific data are lacking.
Aim The aim of the study is to determine the prognostic significance of EMVI in locally advanced rectal cancer on baseline MRI.
Materials and Methods We retrospectively reviewed 117 MRIs of operable non-metastatic locally advanced rectal cancers in a tertiary cancer institute. Three dedicated oncoradiologists determined presence or absence of EMVI, and its length and thickness, in consensus. These patients were treated as per standard institutional protocols and followed up for a median period of 37 months (range: 2–71 months). Kaplan-Meier curves (95% CI) were used to determine disease-free survival (DFS), distant-metastases free survival (DMFS), and overall survival (OS). Univariate analysis was performed by comparing groups with log-rank test.
Results EMVI positive cases were 34/114 (29%). More EMVI-positive cases developed distant metastasis compared with EMVI-negative cases (14/34–41% vs. 22/83–26%). The difference, however, was not statistically significant (p = 0.146). After excluding signet-ring cell cancers (n = 14), EMVI showed significant correlation with DMFS (p = 0.046), but not with DFS or OS. The median thickness and length of EMVI was 6 and 14 mm, respectively in patients who developed distant metastasis, as compared with 5 and 11 mm in those who did not, although this difference was not statistically significant.
Conclusion EMVI is a predictor of distant metastasis in locally advanced non-metastatic, non-signet ring cell rectal cancers. EMVI can be considered another high-risk feature to predict distant metastasis.