bluuebunny/crossref_metadata_2025_split huggingface dataset:
Pregnancy Outcomes Following the First Frozen Blastocyst Transfer among Women Aged Less than 35 Years Old
Authors: Hoang Le , Thang Le Duc , Huong Nguyen Thi Lien , Thuy Nguyen Minh , Anh Vu Thi Mai , Duc Nguyen Thanh , Chi Nguyen Thi Dieu , Dung Tham Chi , hugues jean-noël 
Published: March 2021

Abstract
Aims of the study were to describe main outcomes (clinical, ongoing single and multiple pregnancy and live birth rates) following frozen blastocyst transfer performed for the first time among women aged less than 35 years old and analyzed according to both quantity and quality of the embryos. A descriptive cross-sectional study was applied to collect and analyze available data of 505 patients who performed transfer of frozen blastocysts for the first time between June, 2018 and September, 2019 at the Assisted Reproductive Technology Centre of Tam Anh General Hospital. One good quality embryo was transferred for 121 patients (Group 1), 2 good quality embryos for 214 patients (Group 2), 1 good and 1 poor quality embryo for 112 patients (Group 3), 1 good and 2 poor quality embryos for 25 patients (Group 4) and 1 or 2 poor quality embryos for 33 patients (Group 5). Main results showed that the pregnancy rate was 71.9%, 74.8%, 69.4%, 84.0% and 39.4% in Group 1, 2, 3, 4 and 5, respectively. The rate of multiple pregnancy was 36.9%, 16.9%, and 32.0% in Group 2, 3, and 4, respectively, higher than in Group 1 (4.9%). Meanwhile, the live birth rate was 55.6%, 50.9%, and 60.0% in Group 2, 3 and 4, respectively, but not significantly different from the live birth rate in Group 1 (47.9%). In conclusion, pregnancy and live birth rates were not significantly different following transfer of 1 or 2 good quality blastocysts while the rate of multiple pregnancy was significantly increased following the transfer of 2 good quality ones. Transfer of 1 or 2 poor quality embryos in addition to 1 good embryo did not significantly improve the pregnancy rate.
Shenzhi Jiannao Formula Ameliorates Vascular Dementia in Vivo and in Vitro by Inhibition Glutamate Neurotoxicity via Promoting Clathrin-Mediated Endocytosis
Authors: Tian Danfeng , Guo Yangyang , Zhang Dandan , Gao Qiang , Liu Ganlu , Lin Jingfeng , Chang Ze , Wang Yuchun , Su Rui , Han Zhenyun 
Published: March 2021

Abstract
Background: Synaptic damage and glutamate excitotoxicity have been implicated in the pathogenesis of vascular dementia (VD). Clathrin, RAB5B and N-methyl-d-aspartic acid receptor 1 (NMDAR1) proteins play a vital role in endocytosis of synaptic vesicles in neurons and glutamate over accumulation. Previous researches have been confirmed that Shenzhi Jiannao (SZJN) formula has an anti-apoptotic and neuroprotective effect in VD, but the underlying mechanisms are still unclear. In this study, we aimed to explore the effect of SZJN formula on cognitive impairment and glutamate excitotoxicity via clathrin-mediated endocytosis (CME) in vivo and in vitro. Methods: Both common carotid arteries were permanent occluded (2‐vessel occlusion, 2VO) in male Sprague Dawley (SD) rats to model VD. One day after operation, the rats began daily treatment with SZJN formula for two weeks. The neuroprotective effects of SZJN formula was subsequently assessed by the novel object recognition test, Morris water maze, hematoxylin-eosin (HE) staining and Nissl staining. Glutamate cytotoxicity was assessed by detecting cell viability and cell death of PC12 cells. Immunohistochemistry, immunofluorescence, Western blot, and quantitative real‐time PCR were used to detect the expression levels of clathrin, RAB5B, and NMDAR1.Results: Administration of SZJN formula effectively improved short-term memory and spatial memory. SZJN treatment significantly reduced hippocampal neuronal loss, and recovered the arrangement and morphology of neurons and Nissl bodies. Moreover, SZJN formula promoted the proliferation of PC12 cells and inhibited glutamate-induced cell death. The down-regulation of clathrin and RAB5B, as well as the upregulation of NMDAR1 in the brain induced by 2VO or glutamate was also notably reversed by SZJN formula at both the protein and mRNA levels, which may contribute to SZJN formula induced improved neurological function. Conclusions: Taken together, our findings provide evidence that the neuroprotective effects of SZJN formula in experimental VD maybe mediated through promoting the expression of clathirn-mediated endocytosis and reducing NMDARs‐associated glutamate excitotoxicity. SZJN formula serves as a promising alternative therapy and may be a useful herbal medicine for preventing progression of VD.
Pregnancy Rate After Myomectomy and Associated Factors Among Reproductive Age Women Who Had Myomectomy at Saint Paul’s Hospital Millennium Medical College, Addis Ababa: Retrospective Cross-sectional Study.
Authors: jeldu meseret olana , asress Tadios Mekonnen , arusi temesgen tantu , gutulo Muluken Gunta 
Published: March 2021

Abstract
Introduction: Uterine myoma occurs in 20-50% of reproductive age women. Uterine myomas may be associated with 5-10% of cases of infertility, but it is the sole cause or factor in only 2-3% of all infertility cases. Myomectomy is surgery done to remove myoma regardless of the methods.Objective: to assess impact of myomectomy on pregnancy rate and associated factors among reproductive age women who had myomectomy at St. Paul’s Hospital Millennium Medical College, in Addis Ababa. Methodology: Hospital based retrospective cross-sectional study was conducted to determine pregnancy rate after myomectomy & its associated factors. Patients who had myomectomy in SPHMMC from September, 2012 to September, 2017 were enrolled. Information was retrieved from hospital records & phone interviews with the patients. The strength of statistical association was measured by adjusted odds ratios and 95% confidence intervals. Statistical significance was declared at p-value < 0.05. Result: Among 180 females participated in this study, 52.2% got pregnant after myomectomy. The result showed that females with age >35 years were 0.31 times less likely to get pregnant after surgery than those ages 20-25 years [AOR=0.31(95%CI: 0.29-0.54)]. People with no infertility before surgery were 1.19 times more likely to be pregnant after surgery than those with unexplained infertility before the surgery [AOR=1.19(95%CI: 1.06-1.57)]. People with two uterine incisions were 0.06 times less likely [AOR=0.06(95%CI: 0.043-0.51)] while those with three or more than three incisions were 0.02 times less likely [AOR=0.02(95%CI: 0.002-0.22)] to get pregnant compared with those with one incision on uterine wallConclusion: Age, number of incision and infertility before surgery were significantly associated with rate of pregnancy after myomectomy.
Analysis of Association Between Genetic Heterogeneity of Interferon Beta Receptor Promoter and Therapeutic Response to Interferon-beta in a Patient with Multiple Sclerosis
Authors: Hajian Samin , Mazdeh Mehrdokht , Nouri Fatemeh , Soleimani Meysam 
Published: March 2021

Abstract
Multiple sclerosis (MS) is an autoimmune disease characterized by inflammatory neuronal damages and consequent disabilities. Episodic relapses of the disease could be decreased by the interferon-beta (IFN-β) therapy in most MS patients. However, the drug response’s efficiency is variable among patients, and the precise mechanism of action of the IFN-β is not clear. This study aimed to investigate the interferon beta-receptor (IFNAR) promoter polymorphisms on response to interferon beta in MS patients. Patients were divided into either responding (n = 57) or non-responding (n = 43) groups according to interferon beta treatment and Expanded Disability Status Scale score. The Sanger sequencing method is used for genotyping. Here, we found a significant association between 65 SNP in responders and non-responders to interferon beta (p-value < 0.05). The results also showed a significant difference between the two groups of responders and non-responders to the treatment in the presence or absence of insertion before GT repeat dinucleotide microsatellite (p-value < 0.02). The present study’s obtained results suggested the genetic heterogeneity in the promoter region of IFNAR can affect response to IFN-β. However, more studies with a larger sample size are needed to demonstrate this relationship further.
The Legacy of the Reformasi: The Role of Local Government Spending on Industrial Development in a Decentralized Indonesia
Authors: Wiryawan Bangkit A. , Otchia Christian 
Published: March 2021

Abstract
Starting in 2001 the government of Indonesia employed Regional Autonomy law, providing larger fiscal role to the province and district governments. However, our understanding of its impacts on economic development of Indonesia is still limited. This paper seeks to find the relationship between increasing local government’s capital expenditure on industrial development with focus in the non-oil and gas sector. Capital spending is thought to have moderation effect on investment, the main channel for industrialization, that should contribute to industrial growth. Our System GMM result suggests that there is significant correlation between capital spending and industrial growth. However, we fail to find significance moderation effect between local spending and investment towards the industry. Decentralization progress in Indonesia has been institutionally anchored by the central government particularly with the introduction of concurrent affairs in 2004, allowing central government to take a major developmental role at the local level. In the long run this contributes to the weakening capacity building at the local level, resulting in our non-positive findings. We propose a new institutional model that promote better central-local collaboration.
Post-Traumatic Stress Disorder (PTSD) and Depression in Iranian Adolescents with and Without Hearing Loss (HL) in Previous and During the Outbreak of the COVID-19
Authors: Ariapooran Saeed , Khezeli Mehdi , Batool Ahadi 
Published: March 2021

Abstract
Background: Due to the unavailability of information and resources about COVID-19 in people with Hearing Loss (HL), especially deaf people, the psychological problems, such as PTSD and depression are probably raised in people with hearing loss (HL) during the outbreak of COVID-19. This study was conducted to compare post-traumatic stress disorder (PTSD) and depression in Iranian adolescents with and without HL in previous and during the outbreak of COVID-19. Methods: The statistical sample was 112 adolescents half (56) of whom was with HL, while another half (56) was without HL. The two groups were also homogenized in terms of age, gender, and education. Data were gathered using the Child PTSD Symptom Scale for DSM-5 (CPSS-5) and Children's Depression Inventory Short version (CDI: S). The data obtained were analyzed using two-way MANOVA. Results: Results showed that 46.43% and 41.04% of with-HL adolescents during the outbreak of COVID-19, and 17.87% and 25.00% of them in previous the outbreak of COVID-19 had symptoms of PTSD and depression, respectively. Results indicated that the mean score difference between PTSD and depression during and in previous the outbreak of COVID-19 [(during)-(previous)] was higher in adolescents with HL than the control group. conclusion: We concluded that psychological and medical interventions must be beneficial to decrease symptoms of PTSD and depression in adolescents (especially in deaf and hard-of-hearing adolescents) during the outbreak of COVID-19.
Penta Band Single Negative Meta-Atom Absorber designed on Square Enclosed Star-Shaped Modified Split Ring Resonator for S-, C-, X- and Ku- Bands Microwave Applications
Authors: Islam Md. Rashedul , Islam Mohammad Tariqul , Moniruzzaman Md. , Samsuzzaman Md. , Arshad Haslina 
Published: March 2021

Abstract
This paper represents a penta band square enclosed star-shaped modified split ring resonator (SRR) based single negative meta-atom absorber (MAA) for multi-band microwave regime applications. FR-4 low-cost material has been used as a substrate to make the MAA unit cell with 0.101λ0 × 0.101λ0 of electrical size, where λ0 is the wavelength calculated at the lower resonance frequency of 3.80 GHz. There are two outer square split ring and one inner star ring shape resonator of 0.035 mm thickness of copper placed on the one side, and another side of the substrate has full copper to construct the desired unit cell. The MAA unit cell provides five absorption peaks of 97.87%, 93.65%, 92.66 %, 99.95%, and 99.86% at the frequencies of 3.80, 5.65, 8.45, 10.82, and 15.92 GHz, respectively, which covers S-, C-, X-, and Ku-bands. The properties of MAA have been investigated and analyzed in the E-, H-fields and surface current. The EMR and highest Q factor of the designed MAA is 9.87 and 30.41, respectively, and it shows a single negative (SNG) property. Different types of parametric analysis have been done to show the better performance of absorption. Advanced Designed System (ADS) software has been used for equivalent circuit to verify the simulated S11 result obtained from the CST-2019 software. Experimental outcomes of the MAA unit cell have a good deal with the simulated result and measured result of the 24×20 array of unit cells also shown. Since the unit cell provides superior EMR, excellent Q-factor, and highest absorption so the recommended MAA can be effectively used as a penta band absorber in microwave applications, like notch filtering, sensing, reducing the unintended noise generated with the copper component of the satellite and radar antennas.
In Vitro Screening of Cucmis Melo L. Against Drought Mediated By PEG and Sorbitol
Authors: Mehmandar Maryam Nekoee , Rasouli Farzad , Giglou Mousa Torabi , Zahedi Seyed Morteza , Aazami Mohammad Ali 
Published: March 2021

Abstract
Water deficit in first growth stages of melon (Cucumis melo L.) in formation of first true leaves after germination can be a factor limiting production. The first step for resolve the problem is genotypes evaluation and identification of drought tolerant melons. An effective method to achieve the goal is use of osmotic solution in tissue culture. Responses of Iranian melon landraces to drought was evaluated using sorbitol at 0.1, 0.2 and 0.4 M or polyethyleneglycol (PEG 6000) at 0.009, 0.012 and 0.015 M concentrations, and MS medium without treatment as the control. Coleoptile length, fresh and dry weight of shoots and roots, photosynthetic pigments, protein, proline, malondialdehyde (MDA) and antioxidant enzymes superoxide dismutase, guaiacol peroxidase and ascorbate peroxidase were measured. The PEG or sorbitol decreased coleoptile length, fresh weight and photosynthetic pigments, and led to enhancement of proline and MDA. Contents of protein and antioxidant enzymes was completely dependent on genotype and type and concentration of osmotic material. The in vitro culture for screening and identification of tolerant and sensitive drought genotypes could be rapid, useful and effective, with sorbitol mimicing drought better than PEG. After in vitro evaluation, the genotype responses to induced water deficit need to be confirmed under field conditions.
Antibioresistance and Virulence Factors Spectrum in Several Bacterial Strains Isolated from Polluted Environments
Authors: Cirstea Maria Doina , Neagu Simona , Stefănescu Mugur 
Published: March 2021

Abstract
Most studies concerning antibiotic resistance are performed on strains isolated from the clinical environment. This phenomenon should not be neglected in bacterial strains isolated from natural or polluted environments, because the phenomenon of virulence and antibiotic resistance occurred in these environments and can contribute to maintaining a reservoir of virulence and antibiotic resistance. This study aimed to assess the resistance factors as well as the antibiotic resistance pattern in bacterial strains isolated from environments polluted with oil products or heavy metals.The strains under study exhibited virulence factors, 85% of the strains had the ability to synthesize amylases, 65% to hydrolyze esculin and gelatin, 50% to produce lecithinases, and hemolysins, and 40% to metabolize casein. To some bacterial straina, the blaNDM and blaIMP resistance genes were identified. These microorganisms showed intermediate resistance to Carbapenem, Monobactam and Aminoglycoside antibiotic classes. Pollutants exert selective pressure on native microorganisms contributing to the development of defense mechanisms against pollution stress, these mechanisms can be used in virulence and antibiotic resistance.
Heparin- and Rosuvastatin- loaded Poly(L-lactide-co-caprolactone) Nanofiber Aneurysm Covered Stent Inhibits Inflammatory Smooth Muscle Cell in Reducing in-stent Stenosis and Thrombosis
Authors: Liu Yingjun , Liu Peixi , Song Yaying , Li Sichen , Shi Yuan , Quan Kai , Yu Guo , Li Peiliang , An Qingzhu , Zhu Wei 
Published: March 2021

Abstract
Background: Endovascular covered-stent has unique advantages in treating complex intracranial aneurysms. However, in-stent stenosis and late thrombosis have become the main factors affecting the efficacy of covered-stent treatment. Smooth-muscle-cell phenotypic modulation plays an important role in late in-stent stenosis and thrombosis. Thus, covered stents loaded with drugs that can inhibit smooth-muscle-cell phenotypic modulation may lower the incidence of long-term complications. Methods: Nanofiber covered stents were prepared using coaxial electrospinning. A rabbit-carotid-artery aneurysm model was established and treated with covered stents. Angiography and histology were performed to evaluate the therapeutic efficacy and incidence rate of in-stent stenosis and thrombosis. Phenotype, function, and inflammatory factors of smooth-muscle cells were studied to explore the mechanism of rosuvastatin in smooth-muscle cells. Result: Heparin–rosuvastatin-loaded nanofiber scaffolds inhibited the proliferation of synthetic smooth-muscle cells. Heparin–rosuvastatin-loaded nanofiber covered stent effectively treated aneurysms without showing any notable in-stent stenosis. In vitro experiments showed that rosuvastatin could inhibit the smooth muscle cell phenotypic modulation of platelet-derived growth factor-BB induction. The inflammatory cytokines secretion and cell viability were inhibited after rosuvastatin treatment.Conclusion: Rosuvastatin could inhibit the abnormal proliferation of synthetic smooth-muscle cells. Heparin–rosuvastatin-loaded covered stents could reduce the incidence of stenosis and late thrombosis and improve the healing rates of stents used for aneurysm treatment.
The effectiveness and safety of modified posterior scleral reinforcement in Chinese children with high myopia
Authors: Ouyang Qianru , Miao Zequn , Xu Xin , Guo Lili , Meng Qingyu , Liang Shuting , Zhang Jingyi , Fu Haoli , Cao Yu , Huang Lvzhen , Wang Lejin 
Published: March 2021

Abstract
Background To evaluate the effectiveness and safety of modified posterior scleral reinforcement in controlling the progression of myopia in patients with high myopia and provide reference information on surgical treatment for these patients.Methods The study included 32 patients (55 eyes) with high myopia who were treated with modified posterior scleral reinforcement and were followed up at 3 months, 6 months, 1 year and 2 years after the operation.The axial length, refractive error, best corrected visual acuity (BCVA) and fundus colour photographs after the operation were compared with those before the operation, and whether there were any complications was recorded.Results The axial length of the eye increased by 0.07 mm, 0.12 mm, 0.31 mm and 0.19 mm at 3 months, 6 months, 1 year and 2 years after the operation, respectively. The refractive error increased by 0.05 D, 0.28 D, 0.53 D and 0.50 D at 3 months, 6 months, 1 year and 2 years after the operation, respectively, and the differences were not significant (P > 0.05). There were no statistically significant changes in BCVA, but there was an upward trend. No severe complications occurred.Conclusion Modified posterior scleral reinforcement can effectively delay the worsening of the ocular axis and refractive error, without severe complications in the short term.
Design, validation and implementation of the Post-Acute (long) COVID-19 Quality of Life (PAC-19QoL) instrument in the PAC-19QoLReg patient registry
Authors: Jandhyala Ravi 
Published: March 2021

Abstract
Background

To design, validate and implement a Post-Acute (long) COVID-19 disease-specific quality of life instrument, (the PAC-19QoL), in a dedicated patient registry, PAC-19QoLReg, to observe changes in quaQoL over a 12-month period.
Methods

The Jandhyala Method was used to identify indicators of long COVID quality of life. The resulting PAC-19QoL was validated with a control group of unaffected participants and finally implemented in the dedicated PAC-19QoLReg.
Participants

: 15 participants suffering from long COVID, who have been positively diagnosed with COVID-19, either via diagnostic or antibody tests and a validation control group of 16 healthy participants who have not suffered from COVID-19.
Main Outcome measures:

Indicators submitted by participants with long COVID that address the specific impact of the illness in their quality of life.
Results

Forty-four Quality of Life Indicators (QoLI) across four domains – psychological, physical, emotional and work-related – were agreed by the participants with long COVID to be relevant for the assessment of their quality of life (CI > 0.5). The validation stage identified 35/44 QoLIs that differentiated between the two groups, with a statistically significant difference between the mean QoLI Likert Scores (P < 0.05).
Conclusions

The PAC-19QoL instrument and PAC-19QoLReg prospective observational cohort clinical study will enable an understanding of disease progression, on and off treatment, on the quality of life of patients with long COVID beyond simple symptomatology.
Trial registration:

ClinicalTrials.gov Identifier NCT04586413; 14th October 2020