bluuebunny/crossref_metadata_2025_split huggingface dataset:
CircStrn3 Targeting microRNA-9-5p Is Involved in the Regulation of Cartilage Degeneration and Subchondral Bone Remodeling in Osteoarthritis
Authors: Li Bin , Ding Tao , Chen Haoyi , Li Changwei , Chen Bo , Xu Xing , Huang Ping , Hu Fangqiong , Guo Lei 
Published: November 2021

Abstract
Background: Osteoarthritis (OA) is the most frequent chronic degenerative joint disease, which is a “whole joint” disease including the pathological changes in the cartilage, subchondral bone and the synovium. Mechanical instability is the initiation of the development of OA. Methods: Minus RNA sequencing, fluorescence in situ hybridization and quantitative real-time PCR were used to detect the expression of circStrn3 in human and mouse OA cartilage tissues and chondrocytes. Stimulate chondrocytes to secrete exosomes miR-9-5p by stretching strain. Intra-articular injection of exosomes miR-9-5p into the OA model induced by the operation of instability of the medial meniscus in mice.Results: In the present study, minus RNA sequencing data showed that tensile strain could decrease the expression of circStrn3 in chondrocytes. The results of fluorescence in situ hybridization and quantitative Real-time PCR showed that circStrn3 expression was significantly decreased in human and mouse OA cartilage tissues and chondrocytes. CircStrn3 could inhibit matrix metabolism of chondrocytes through competitively 'sponging' miRNA-9-5p targeting kruppel-like factor 5 (KLF5), indicating that the decreasing of circStrn3 might be a protective factor in mechanical instability-induced OA. Further studies showed that the tensile strain stimulated chondrocytes to secrete exosomes miR-9-5p. Exosomes with high miR-9-5p expression from chondrocytes could inhibit osteoblasts differentiation by targeting KLF5. In addition, intra-articular injection of exosomal miR-9-5p obviously alleviated the progression of OA induced by destabilized medial meniscus surgery in mice. Conclusions: Taken together, these results demonstrated that the reduction of circStrn3 caused the increasing of miR-9-5p, which acted as a protective factor in mechanical instability-induced OA and provided a novel mechanism of communication among joint components and a potential application for the treatment of OA.
High Triglyceride-Glucose Index (TyG) is Associated With Adverse Cardiovascular Events in Patients With Type 2 Diabetes Mellitus (T2DM) and Chronic Total Occlusion (CTO) After Percutaneous Coronary Intervention (PCI)
Authors: Lin Xiao Long , Sun Hao Xuan , Zhao Jin Yang , Li Fan Qi , Zhao Dong Hui , Ma Qin , Liu Jing Hua , Fan Qian 
Published: November 2021

Abstract
Background: The triglyceride-glucose index (TyG index) is a reliable surrogate marker of insulin resistance, which is associated with cardiovascular disease morbidity and prognosis. However, the predictive value of the TyG index for cardiovascular events in patients with type 2 diabetes mellitus (T2DM) and chronic total occlusion (CTO) after percutaneous coronary intervention (PCI) has not been specifically studied. Method: The study retrospectively enrolled 687 patients with T2DM and CTO after PCI in the final analysis. Patients were divided into three groups according to the TyG index tertiles. The TyG index was calculated as ln [ fasting triglyceride (mg/dL) × fasting glucose (mg/dL)/2 ]. The primary observational endpoint was the composite of overall death, non-fatal stroke, non-fatal myocardial infarction (MI), or unplanned revascularization.Results: During a median follow-up of 22.0 months, 159 patients (23.14%) experienced primary endpoint events. Multivariate Cox hazards regression analysis showed that the TyG index was significantly correlated with the primary endpoint [HR 2.827, 95% CI (1.877-4.529), P＜0.001]. Kaplan–Meier curves for the primary endpoint showed a significant difference between the lower and higher TyG index group was mainly driven by the increased incidence of unplanned revascularization (Log-rank P＜0.001).Conclusion: The TyG index may be a remarkable predictor of adverse cardiovascular events, especially unplanned revascularization in patients with T2DM and CTO who are treated by PCI.
Prevalence and Determinants of Depression, Anxiety and Stress Among Psychiatric Nurses in Ghana: a Cross Sectional Study
Authors: Agyemang Sampson Opoku , Ninnoni Jerry Paul , Enyan Nancy Innocentia Ebu 
Published: November 2021

Abstract
Background: The job demands and stress associated with the nursing profession put nurses on an escalating risk to exhibit psychiatric morbidity such as anxiety and depression. This study assessed the prevalence and risk factors of depression, anxiety and stress among nurses working in public psychiatric hospitals in Ghana. Methods: A cross-sectional design was used for this study. Proportionate and simple random sampling method were used to select 311 respondents for the study. Data was collected using a questionnaire which comprised Beck’s Depression Inventory, Beck’s Anxiety Inventory and Perceived Stress Scale. Data was analysed using descriptive statistics and a multinomial logistic regression. Results: The results showed that 92% and 96.5% of mental health nurses experienced minimal depression and low anxiety respectively. In addition, 56.6% and 42.8% experienced low and moderate stress respectively. There was a positive association between educational level and depression whereas, a negative association existed between educational level and anxiety and stress. Also, mental health nurses who work at the OPD and Administration were more likely to experience anxiety and stress. Conclusion: The findings of this study suggest that, higher level of educational opportunities for mental health nurses leads to decreases anxiety among nurses. Also, the work environment needs to be made conducive to promote the psychological wellbeing of nurses. Additionally, interventions on the management of depression, anxiety and stress by nurses in mental health hospitals need to be instituted, thereby, improving the health of nurses working in mental health hospitals.
Postoperative complication of the index surgery is a risk factors for postoperative ileus after diverting loop ileostomy closure: a retrospective study
Authors: Nakao Toshihiro , Shimada Mitsuo , Yoshikawa Kozo , Tokunaga Takuya , Nishi Masaaki , Kashihara Hideya , Takasu Chie , Wada Yuma , Yoshimoto Toshiaki , Yamashita Syoko , Iwakawa Yosuke 
Published: November 2021

Abstract
Background: Postoperative ileus is one of the most common complications after diverting loop ileostomy closure. Some reports have investigated the risk factors for postoperative complications or ileus after ileostomy closure; however, these studies did not evaluate the index surgery sufficiently. In this study, we evaluated the risk factors, including the details of the index surgery, for ileus after diverting ileostomy closure.Methods: This was a retrospective study of patients who underwent ileostomy closure following index surgery for rectal cancer. Patients who developed postoperative ileus (POI (+)) and patients who did not (POI (−)) after ileostomy closure were compared.Results: Fifty-nine patients were evaluated and were divided into two groups: POI (+) (n=9) and POI (−) (n=50), and the groups were compared. There were no significant differences in the details of the index surgery, operative procedure, transanal total mesorectal excision, lateral lymph node dissection, operating time, or blood loss. The incidence of Clavien–Dindo grade ≥ III complications after the index surgery was significantly higher in the POI (+) group. Conclusions: The incidence of Clavien–Dindo grade ≥ III complications after the index surgery may increase the risk of postoperative ileus after ileostomy closure.
Engrafted glial progenitor cells yield long-term integration and sensory improvement in aged mice
Authors: Yang Zhiqi , Gong Mingyue , Jian Tingliang , Li Jin , Yang Chuanyan , Ma Qinlong , Deng Ping , Wang Yuxia , Huang Mingzhu , Wang Haoyu , Yang Shaofan , Chen Xiaowei , Yu Zhengping , Wang Manxia , Chen Chunhai , Zhang Kuan 
Published: November 2021

Abstract
Aging causes astrocyte morphological degeneration and functional deficiency, which impairs neuronal functions. Until now, whether age-induced neuronal deficiency could be alleviated by engraftment of glial progenitor cell (GPC) derived astrocytes remained unknown. In the current study, GPCs were generated from embryonic cortical neural stem cells in vitro and transplanted into the brains of aged mice. Their integration and intervention effects in the aged brain were examined 12 months after transplantation. Results indicated that these in-vitro-generated GPC-derived astrocytes possessed normal functional properties. After transplantation they could migrate, differentiate, achieve long-term integration, and maintain much younger morphology in the aged brain. Additionally, these GPC-derived astrocytes established endfeet expressing aquaporin-4 (AQP4) and ameliorate AQP4 polarization in the aged neocortex. More importantly, age-dependent sensory response degeneration was reversed by GPC transplantation. This work demonstrates that rejuvenation of the astrocyte niche is a promising treatment to prevent age-induced degradation of neuronal and behavioral functions.
Partial Cystectomy Could Be a Better Option of Bladder Preservation in T1 High-Grade Bladder Cancer Patients: A Propensity Score Matching Study
Authors: Zhan Xiangpeng , Fu Bin 
Published: November 2021

Abstract
Purpose: Partial cystectomy was investigated as a method of bladder preservation with better disease outcomes than transurethral bladder tumor resection in T1 high-grade bladder cancer patients.Method and materials: The national Surveillance, Epidemiology, and End Results database（SEER） (2004-2015) was used to obtain patients diagnosed with T1 high-grade bladder cancer, and finally, 25263 patients were enrolled in our study. The Kaplan-Meier method with the log-rank test was performed to analyze the outcome of overall survival (OS) and cancer-specific survival (CSS) between patients undergoing PC, TURBT, or RC. Moreover, the propensity score matching (PSM) and multivariable Cox proportional hazard model were also utilized in the study.Results: Ultimately, 24635 patients were undergoing TURBT, while 190 and 438 patients were respectively assigned to PC and RC groups. Compared with patients with TURBT, a tendency of a higher proportion of higher older and male patients was observed in the PC group. When matching with RC patients, patients in the PC group were common older and had bigger tumor sizes and single tumor. (All P<0.05). After 1:1 PSM, 190 patients with TURBT and 160 patients receiving RC were selected. In survival analysis, the patients in the PC group had a higher survival probability of both OS and CSS before and after PSM compared with those in the TURBT group. Meanwhile, no significant differences were observed between the RC and PC groups in OS and CSS analysis. Moreover, multivariable cox regression showed that PC was a protective factor for overall mortality (OM) and cancer-specific mortality (CSM) compared with TURBT in T1 high-grade patients. (All P<0.05)Conclusion: Patients undergoing partial cystectomy were shown to have a better outcome compared to those with transurethral bladder tumor resection in T1 high-grade bladder cancer patients. Partial cystectomy was a worthwhile alternative for T1 high-grade bladder cancer.
Impact of Keratoconus Stage on Outcome After Corneal Crosslinking
Authors: Gassel Caroline Julia , Röck Daniel , Konrad Eva-Maria , Blumenstock Gunnar , Bartz-Schmidt Karl Ulrich , Röck Tobias 
Published: November 2021

Abstract
Background: This study aimed to analyze if the outcome after corneal crosslinking (CXL) in progressive keratoconus patients depends on the stage in which the procedure is performed. This knowledge would help to improve success of CXL and facilitate defining surgery indications in those patients.Methods: In this retrospective study, 124 consecutive eyes of 100 patients with progressive keratoconus undergoing corneal CXL at the University Eye Hospital Tübingen were included. The eyes were graded according to modified Krumeich stages depending on induced myopia or astigmatism, corneal radii, minimum corneal thickness, and morphological changes. The observation period took place from November 2008 to September 2018. Preoperatively, 12 and 24 months after CXL, the best corrected visual acuity (BCVA) was determined and astigmatism as well as keratometric parameters (Kmax, Kmin, central corneal thickness (CCT), minimum corneal thickness (MCT)) were measured by means of a Scheimpflug camera system.Results: BCVA results showed significant differences between the modified Krumeich stages 12 months (p=0.014) and 24 months postoperatively (p=0.032). Also, astigmatism differed significantly among the stages 24 months after CXL (p=0.023); however, after 12 months, no significant differences between the groups were detectable. In terms of Kmax, Kmin, CCT, and MCT, no significant differences between the Krumeich stages were observed.Conclusions: BCVA showed a significantly higher improvement after CXL in the early stage of keratoconus compared to a higher stage. However, the postinterventional keratometric values did not differ significantly between the different modified Krumeich stages. The significantly higher improvement in BCVA after CXL in the early stage might indicate that earlier intervention provides a higher subjective benefit to the individual. Further studies with larger sample sizes are needed to confirm these findings.
Effects of Dietary Palmitoleic Acid on Vascular Function in Aorta of Diabetic Mice
Authors: Takenouchi Yasuhiro , Seki Yoshie , Shiba Sachiko , Ohtake Kazuo , Nobe Koji , Kasono Keizo 
Published: November 2021

Abstract
BackgroundChronic hyperglycemia in diabetes causes atherosclerosis and progresses to diabetic macroangiopathy, and can lead to coronary heart disease, myocardial infarction and cerebrovascular disease. Palmitoleic acid (POA) is a product of endogenous lipogenesis and is present in fish and vegetable oil. In human and animal studies, POA is reported as a beneficial fatty acid related to insulin sensitivity and glucose tolerance. However, few studies have reported its effects on aortic function in diabetes. Here, we investigated the effects of POA administration on vascular function in KKAy mice, a model of type 2 diabetes.MethodsMale KKAy and C57BL/6J mice at the age of 14 weeks were used in the present study. For each mouse strain, one group was fed with normal diet and the other group was fed POA-containing diet for 2 weeks. Then, the vascular reactivities of prepared aortic rings were measured in an organ bath to determine if POA administration changed vascular function in these mice.ResultsKKAy mice treated with POA exhibited decreased plasma glucose levels compared with mice treated with normal diet. However, endothelium-dependent vasorelaxant responses to acetylcholine and protease-activated receptor 2 activating protein, which are attenuated in the aorta of KKAy mice compared to control C57BL/6J mice under a normal diet, were not affected by a 2-week POA treatment. In addition, assessment of vasoconstriction revealed that the phenylephrine-induced vasoconstrictive response was enhanced in KKAy mice compared to control mice under a normal diet, but no effect was observed in KKAy mice fed a POA-containing diet. In contrast, there was an increase in vasoconstriction in control mice fed the POA-containing diet compared to mice fed a normal diet. Furthermore, the vasoconstriction in aorta in both control and KKAy mice fed a POA-containing diet were further enhanced under hyperglycemic conditions compared to normal glucose conditions in vitro. In the hyperinsulinemic, and hyperinsulinemic combined with hyperglycemic conditions, vasoconstriction was increased in KKAy mice fed with POA.ConclusionThese results suggest that POA intake enhances vasoconstriction under hyperglycemic and hyperinsulinemic conditions, which are characteristics of type 2 diabetes, and may contribute to increased vascular complications in diabetes.
Protection offered by COVID-19 vaccines in reducing SARS-CoV-2 infection frequency; severity and mortality, among Indian Healthcare Workers: Multicenter, pan-Fortis study
Authors: Acharya Subrat , Mahindra Gaurav 
Published: November 2021

Abstract
During COVID-19 pandemic, Healthcare Workers (HCWs) were at increased risk for exposure to SARS-CoV-2 virus and prioritized for early administration of COVID-19 vaccines in India. Real-life scenario information among vaccinated HCWs acquiring COVID-19 infection, is scarce. We retrospectively analyzed COVID-19 infection frequency, severity, and associated mortality among healthcare workers, immunized with either Covishield or Covaxin vaccines at 27 Fortis Hospitals across 11 Indian states. Positive cases were identified based on RT-PCR or rapid antigen tests for SARS-CoV-2 between 16th January 2021 till 15th May 2021. 20034 HCWs received vaccination. 3971 received 1 dose, 16063 received 2 doses. Post-vaccination, 1139 HCWs acquired COVID-19 infection, 180 (4.53%) and 959 (5.97%) among partially and fully vaccinated category, respectively. Breakthrough infection occurred among 913 (5.68%) HCWs. Concurrently, Case Positivity Rate was 11.9%, among general population (control). Among 1139 positive cases, mild, moderate, and severe infections were 1059 (93%), 71 (6.2%) and 9 (0.8%), respectively with Case Fatality Rate of 0.18%, compared to 0.92% among general population (p=0.0043). The Case Fatality Rate in vaccinated HCWs was found to be 80% less than that in general population (control). Hence, COVID-19 vaccines available in India seem to be effective against SARS-CoV-2 virus.
Effect of serum bisphenol A and low T3 on short-term prognosis of young patients with intracerebral hemorrhage
Authors: wang guoping , Chen Jinhua , Qiao Zhongdong , Zhao Lili , Guo Dongxing , Guo Ping , Wang Aiven , Sun Wanli , Zhang Lifang , Feng Cueiping , Lyu Jiyuan 
Published: November 2021

Abstract

BackgroundBisphenol A (BPA) is widely present in daily necessities, and its exposure is almost everywhere. We aimed to evaluate the relationship between serum BPA levels, biomarkers and recent mortality in young patients with intracerebral hemorrhage (YICH) and low T3 .MethodsUsing a multi-center longitudinal prospective study, the research team recruited 600 YICH patients and divided into two subgroups according to their thyroid function, of which 320 (53.3%) were in the LT3, Sub-clinical hypothyroidism (SCH) was 280 (46.6%) and control group (n = 600).All patients received serum BPA, and biomarker blood draws.ResultsCalculating the cumulative quantification of plastic bag products, BPA increased by 0.204 standard deviation unit per log unit (P<0.003). Serum BPA, thrombin and matrix metalloproteinase (MMP-9) {BPA (0.91±0.14) vs (0.765±0.13) vs. (0.535±0.11) pg/ml, thrombin (1.35±0.18) vs (0.66±) 0.15) vs (0.66 ±0.15) ng/ml, MMP-9 (104±10 vs (53±8.1) vs (53±) 8.1) pg/ml, both P<0.01}. Linear correlation analysis showed that serum BPA was positively correlated with miR-388-3p and 24hDBPCV, and negatively correlated with LT3 and miR-451. Multiple linear regression showed that BPA affected the increase of nDBP Percentage of independent risk factors (β = 0.286), P <0.01. Mortality rate showed 2 ,15 and 30 days of patients in the LT3 and the SCH of 4.7%vs 2.5%,6.25% vs 3.75%,4.68%vs 2.5%, P<0.01.ConclusionHigh BPA exposure strongly predicts the risk of ICH in young patients. The activation of NF-κB signaling pathway, secondary thyroid function, ambulatory blood pressure scores, and changes in messenger ribonucleic acid construct an inflammatory response system. There are also significant differences in recent mortality between the LT3 group and the SCH group.Trial registrationThe trial is registered at clinical .gov (www.medresman.Chi CTR1900023626)
Application of Ultrasonography in Conservative Treatment of Humeral Shaft Fracture in Children
Authors: Li Jun , Tan Xin , Li Xiang , Gou Pan , Yuan Xiaowei , Liu Tao , Shi Mingyan , Liu Xing 
Published: November 2021

Abstract
Purpose: Most of humeral shaft fractures in children could be treated satisfactorily by conservative treatment. This study aimed to evaluate the application value of ultrasonography in conservative treatment of humeral shaft fracture in children.Methods: We retrospectively reviewed children admitted to our hospital, for humeral shaft fracture from January 2014 to March 2018. The patients were divided into two groups: ultrasonography group and X-ray group. All patients were instructed to re-examined at 1 week, 2 weeks, 4 weeks, 12 weeks, 24 weeks and 1 year from the injury date for follow-up, and evaluated the prognosis according to the Disabilities of Arm, Shoulder and Hand (DASH) score at 12 months.Results: A total of 37 children were divided into ultrasonography group, 32 children were divided into X-ray group. There was no significant difference in prognosis between the two groups. The mean number of radiation exposures of ultrasonography group was less and ultrasonic examination found radial nerve injury, and using ultrasound reduced the chance of direct or in direct contact with infected peoples.Conclusion: Ultrasound is a non-invasive, non-radioactive test, which could decrease risk of COVID-19 infection and detect the vascular nerve injury caused by fracture. Ultrasonography may be the examination method for follow-up conservative treatment of humeral shaft fracture in children during the epidemic period.
Gut Dysbiosis and Hemodynamic Changes as Links of the Pathogenesis of Complications of Cirrhosis
Authors: Maslennikov Roman , Ivashkin Vladimir , Efremova Irina , Poluektova Elena , Shirokova Elena , Ivashkin Konstantin , Kudryavtseva Anna , Krasnov George 
Published: November 2021

Abstract
Background. Hemodynamic changes (hyperdynamic circulation) and gut dysbiosis are observed in cirrhosis. It was suggested that gut dysbiosis contributes to the development of hyperdynamic circulation, which aggravates the course of cirrhosis. The aim is to test this hypothesis.Methods. The cross-sectional observational study included 47 patients with cirrhosis. Stool microbiome was assessed using 16S rRNA gene sequencing. Echocardiography with a simultaneous assessment of blood pressure and heart rate was performed. Hemodynamic parameters were calculated. Results. Hyperdynamic circulation was found in 34% of patients. Patients with hyperdynamic circulation had higher incidences of clinically significant ascites (p=0.018), overt hepatic encephalopathy (p=0.042), hypoalbuminemia (p=0.011), hypoprothrombinemia (p=0.019), systemic inflammation (p=0.002), and severe hyperbilirubinemia (p=0.042) than patients without hyperdynamic circulation. The abundance of Proteobacteria (p=0.012), Enterobacteriaceae (p=0.008), Bacilli (p=0.027), Streptococcaceae (p=0.044), Lactobacillaceae (p=0.034), Enterococcaceae (p=0.046), and Fusobacteria (p=0.026) increased and the abundance of Bacteroidetes (p=0.049) and Erysipelotrichia (p=0.029) decreased in the gut microbiome of patients with hyperdynamic circulation compared to patients without hyperdynamic circulation. The systemic vascular resistance value negatively correlated with the abundance of Proteobacteria (r=-0.423; p=0.003), Enterobacteriaceae (r=-0.417; p=0.004), and Fusobacteria (r=-0.401; p=0.005). Heart rate was negatively correlated with the abundance of Bacteroidetes (r=-0.453; p=0.001). The cardiac output value was positively correlated with the abundance of Proteobacteria (r=0.402; p=0.003), Enterobacteriaceae (r=0.424; p=0.003), Fusobacteria (r=0.281; p=0.049), and Bacilli (r=0.314; p=0.031), and negatively correlated with the abundance of Bacteroidetes (r=-0.313; p=0.032) and Erysipelotrichia (r=-0.329; p=0.024). Conclusion. Gut dysbiosis is associated with hyperdynamic circulation, which is associated with a number of complications of cirrhosis.