bluuebunny/crossref_metadata_2025_split huggingface dataset:
A streamlined whole blood CyTOF workflow defines a circulating immune cell signature of COVID‐19
Authors: Geanon Daniel , Lee Brian , Gonzalez‐Kozlova Edgar , Kelly Geoffrey , Handler Diana , Upadhyaya Bhaskar , Leech John , De Real Ronaldo M. , Herbinet Manon , Magen Assaf , Del Valle Diane , Charney Alexander , Kim‐Schulze Seunghee , Gnjatic Sacha , Merad Miriam , Rahman Adeeb H. 
Published: January 2021

AbstractMass cytometry (CyTOF) represents one of the most powerful tools in immune phenotyping, allowing high throughput quantification of over 40 parameters at single‐cell resolution. However, wide deployment of CyTOF‐based immune phenotyping studies are limited by complex experimental workflows and the need for specialized CyTOF equipment and technical expertise. Furthermore, differences in cell isolation and enrichment protocols, antibody reagent preparation, sample staining, and data acquisition protocols can all introduce technical variation that can confound integrative analyses of large data‐sets of samples processed across multiple labs. Here, we present a streamlined whole blood CyTOF workflow which addresses many of these sources of experimental variation and facilitates wider adoption of CyTOF immune monitoring across sites with limited technical expertise or sample‐processing resources or equipment. Our workflow utilizes commercially available reagents including the Fluidigm MaxPar Direct Immune Profiling Assay (MDIPA), a dry tube 30‐marker immunophenotyping panel, and SmartTube Proteomic Stabilizer, which allows for simple and reliable fixation and cryopreservation of whole blood samples. We validate a workflow that allows for streamlined staining of whole blood samples with minimal processing requirements or expertise at the site of sample collection, followed by shipment to a central CyTOF core facility for batched downstream processing and data acquisition. We apply this workflow to characterize 184 whole blood samples collected longitudinally from a cohort of 72 hospitalized COVID‐19 patients and healthy controls, highlighting dynamic disease‐associated changes in circulating immune cell frequency and phenotype.
Dbx2 regulation in limbs suggests interTAD sharing of enhancers
Authors: Beccari Leonardo , Jaquier Gabriel , Lopez‐Delisle Lucille , Rodriguez‐Carballo Eddie , Mascrez Bénédicte , Gitto Sandra , Woltering Joost , Duboule Denis 
Published: January 2021

AbstractBackgroundDuring tetrapod limb development, the HOXA13 and HOXD13 transcription factors are critical for the emergence and organization of the autopod, the most distal aspect where digits will develop. Since previous work had suggested that the Dbx2 gene is a target of these factors, we set up to analyze in detail this potential regulatory interaction.ResultsWe show that HOX13 proteins bind to mammalian‐specific sequences at the vicinity of the Dbx2 locus that have enhancer activity in developing digits. However, the functional inactivation of the DBX2 protein did not elicit any particular phenotype related to Hox genes inactivation in digits, suggesting either redundant or compensatory mechanisms. We report that the neighboring Nell2 and Ano6 genes are also expressed in distal limb buds and are in part controlled by the same Dbx2 enhancers despite being localized into two different topologically associating domains (TADs) flanking the Dbx2 locus.ConclusionsWe conclude that Hoxa13 and Hoxd genes cooperatively activate Dbx2 expression in developing digits through binding to mammalian specific regulatory sequences in the Dbx2 neighborhood. Furthermore, these enhancers can overcome TAD boundaries in either direction to co‐regulate a set of genes located in distinct chromatin domains.
Facile Fabrication of Ag Nanocrystals Encapsulated in Nitrogen‐doped Fibrous Carbon as an Efficient Catalyst for Lithium Oxygen Batteries
Authors: Liu Lili , Ma Tianyi , Fang Weiwei , Liu Yuqing , Konstantinov Kostantin , Wang Jiazhao , Liu Hua‐Kun 
Published: January 2021

A facile synthesis of Ag nanocrystals encapsulated in nitrogen‐doped carbon fiber (NCF) is proposed, based on the simultaneous reaction between pyrrole and Ag+ ions in an aqueous solvent followed by a heat treatment. The as‐prepared Ag/NCF demonstrated superior catalytic behavior toward ORR and OER. Besides improved cycling stability, a much lower discharge/charge gap of 0.89 V (vs Li/Li+) compared with 1.38 V for NCF cathode with a fixed capacity of 500 mAh g−1 was obtained in lithium oxygen batteries. The introduction of Ag crystals into NCF facilitates the oxygen reduction reaction/oxygen evolution reaction kinetics. X‐ray diffraction analysis coupled with Raman spectroscopy confirmed that Ag/NCF cathode could reversibly catalyze Li2O2 formation and decomposition. The NCF matrix offers a conductive network to realize rapid mass transfer and the encapsulated Ag nanocrystals supplied effective catalytic active sites. The combined action between both contributes to the superior electrocatalytic performance.
The prevalence, predictors, and outcomes of spontaneous echocardiographic contrast or left ventricular thrombus in patients with HFrEF
Authors: Zhou Xiaodong , Shi Ruiyu , Wu Gaojun , Zhu Qianli , Zhou Changzuan , Wang Liangguo , Xue Chenglong , Jiang Yuanyuan , Cai Xueli , Huang Weijian , Shan Peiren 
Published: January 2021

AbstractAimsThis study aimed to determine prevalence, predictors, and association with ischaemic stroke risk of spontaneous echocardiographic contrast (SEC) or left ventricular thrombus (LVT) in patients with heart failure with reduced ejection fraction (HFrEF).Methods and resultsClinical, echocardiographic, and follow‐up data from January 2009 through February 2019 were retrospectively extracted from electronic medical records of patients with heart failure with left ventricular ejection fraction < 40% by echocardiography on admission, with follow‐up to February 2020. Of 9485 consecutive patients with HFrEF, 123 (1.3%) presented LVT and 331 (3.5%) presented SEC. Patients with vs. those without SEC/LVT had larger left ventricular end‐diastolic volume (199.5 ± 77.7 vs. 165.8 ± 61.3 mL, P < 0.001), lower left ventricular ejection fractions (29.5 ± 7.0% vs. 33.7 ± 5.5%, P < 0.001), and more often ischaemic cardiomyopathy, apical aneurysm, chronic kidney diseases, and smoking habit. In Cox regression analysis, SEC and LVT were independent predictors for ischaemic stroke occurrence [hazard ratio (HR) = 2.40, 95% confidence interval (CI): 1.74–3.31; HR = 4.52, 95% CI: 2.77–7.40, both P < 0.001]. In patients with those without SEC or LVT, stroke risk was higher among those not on anticoagulants (HR = 2.55, 95% CI: 1.85–3.53; HR = 4.71, 95% CI: 2.84–7.81, both P < 0.001), but similar among those on anticoagulants (P > 0.05). In patients with sinus rhythm, the associations between SEC/LVT and ischaemic stroke persist with HRs of 2.57 (95% CI: 1.69–3.92) and 5.74 (95% CI: 3.38–9.75).ConclusionsIn patients with HFrEF, SEC was not uncommon and increased risk of ischaemic stroke as well as LVT. Anticoagulants could play a role in the reduction of stroke risk, suggesting that patients with SEC/LVT, even those in sinus rhythm, would benefit from systemic anticoagulation treatment.
Label‐free Selective Detection of Protein Markers in the Picomolar Range via a Convenient Voltammetric Sensing Strategy
Authors: Chen Chun‐Yu , Lehr Joshua 
Published: January 2021

AbstractA convenient strategy for the preparation of label‐free selective protein biomarker sensors is presented. After formation of a ferrocene terminated monolayer on a gold electrode using thiol chemistry, grafting of an antifouling phenylalanine layer onto the preformed ferrocene monolayer is achieved by diazonium chemistry. This provides a platform for receptor (anti‐BSA or anti‐CRP) immobilization as well an antifouling interface. Specific target binding leads to attenuation of the voltammetric faradaic signal of the underlying ferrocene group. Thereby, label‐free, selective, voltammetric detection is achieved with highly competitive limits of detection in the low (≤4) picomolar range for both BSA and CRP.
Increased DNA strand breaks and neoplastic transformation in human bladder cells treated with pioglitazone
Authors: Atlı Şekeroğlu Zülal , Şekeroğlu Vedat , Kontaş Yedier Seval , İlkun Emre , Liou Louis S. 
Published: January 2021

AbstractPioglitazone (PIO), an oral hypoglycemic agent, is used in the treatment of type 2 diabetes. Some studies have suggested that an increased risk of bladder cancer with PIO exposure, while the others reported there is no such relationship. Therefore, it is doubtful whether PIO can increase the risk of bladder cancer. The effects of PIO on DNA damage and/or transformation of human bladder cells are not fully known. We investigated the effects of PIO on cytotoxicity, DNA single and double strand breaks and repair and neoplastic transformation in human bladder cells (hTU1) treated with 10, 20, and 40 μM PIO for 24, 48 and 72 hr. PIO decreased cell viability in a concentration‐dependent manner. Increased levels of comet parameters showed that PIO and its metabolites can significantly induce DNA double strand breaks at all concentrations tested. PIO also significantly induced the formation of phosphorylated H2AX and p53 binding protein 1 foci. DNA damage was not repaired in a 24 hr recovery period. PIO can also induce malignant transformation of human bladder cells exhibiting loss of contact inhibition and anchorage independent growth. This is the first study to indicate that PIO can induce DNA damage and malignant transformation, reduce or alter the DNA repair capacity in human bladder cells. From these results, we suggest that patients with diabetes treated with PIO may have an increased risk of bladder cancer.
Micronucleated erythrocytes in peripheral blood from neonate rats fed by nursing mothers exposed to X‐rays
Authors: Solórzano‐Meléndez Alejandro , Rodrigo‐Alarcón Rodolfo , Gómez‐Meda Belinda C. , Zamora‐Pérez Ana L. , Ortiz‐García Ramón G. , Bayardo‐López Luis H. , González‐Virgen Roberto , Gallegos‐Arreola Martha P. , Zúñiga‐González Guillermo M. 
Published: January 2021

AbstractMost women with breast cancer can become pregnant and give birth while undergoing radiation therapy and breastfeeding is generally not contraindicated. The induction of long‐lived reactive species in proteins, such as casein by X‐ray radiation and DNA damage to unexposed organisms, has been shown when ingesting irradiated cheese. To determine whether exposing lactating rats to X‐rays increases the number of micronucleated erythrocytes (MNEs) in peripheral blood of their unexposed or breastfeeding rat pups, 15 female Wistar rats were divided into three groups: Negative control; Experimental group exposed to X‐rays, and group exposed to X‐rays plus vitamin C. The mothers of groups 2 and 3 were irradiated for three consecutive days after giving birth, returning them to their respective cages each time to continue lactation. A blood sample was taken from the mothers and pups at 0, 24, and 48 hr. Blood smears were stained with acridine orange to analyze MNEs. In mother rats, the frequency of micronucleated polychromatic erythrocytes (MNPCEs) increased significantly at 24 and 48 hr in both study groups exposed to radiation. Likewise, in rat pups the MNPCE and MNE frequencies increased in both groups with radiation and radiation plus vitamin C at 24 and 48 hr, and a protection from vitamin C was observed. In conclusion, the genotoxic damage produced in rat pups that were lactated by mothers irradiated with X‐rays is possibly due to the effect of long‐lived reactive species that were formed in the breast milk of female Wistar rats during the irradiation process.
Does reduced intraspecific competition of the dominant species in intercrops allow for a higher population density?
Authors: Wang Qi , Bai Wei , Sun Zhanxiang , Zhang Dongsheng , Zhang Yue , Wang Ruonan , Evers Jochem B. , Stomph Tjeerd‐Jan , van der Werf Wopke , Feng Chen , Zhang Lizhen 
Published: January 2021

AbstractDominant species in intercropping experience less resource competition compared with its monoculture. This reduced competition for resources may allow cultivating the dominant species at an increased density in intercropping to obtain greater yield. However, experimental results are inconclusive when the optimal within row density in the sole crop is not well established. Here, we conducted a two‐year experiment to test the hypothesis that optimal within row plant density of dominant species in intercropping would be higher in the intercrop than in the sole crop. We tested three maize densities (3, 4.5, and 6 plants m−1) in both sole maize and two replacement designed intercrops. The row configurations of two intercrops are two rows maize intercropped with four rows peanut (M2P4) and four rows maize intercropped with four rows peanut (M4P4). Peanut was grown at the same plant density of 12 plants m−1 row in both sole crop and intercrops. The results indicated that increasing maize density from the optimal density in monoculture is not worthy of promotion to improve yield in intercropping, which denied our hypothesis. The land equivalent ratios (LER) in the dry year (2017) were higher than the wet year (2016). Maize yields per unit area of the whole intercropping system were highest with densities of 4.5 and 6 plants m−1 row, with no significant difference between these two densities. Maximum maize yields in sole cropping were obtained with maize densities of 6 plants m−1 row. Intercropping provided higher yields at low and intermediate sole crop maize densities, but not at high sole crop maize density. Average land equivalent ratios at 3, 4.5, and 6 plants m−1 of maize were 1.09, 1.04, and 0.95 in 2016, and 1.07, 1.10, and 1.02 in 2017. Our results suggest that intercropping performs better at conditions with less resources than adequate resources.
Empirical Evidence for Depensation in Freshwater Fisheries
Authors: Sass Greg G , Feiner Zachary S , Shaw Stephanie L 
Published: January 2021

Abstract
Inland fisheries face increasing threats to their sustainability. Despite speculation that depensation may exacerbate the effects of stressors on population resiliency, depensation has not been empirically explored in freshwater fisheries. Declining productivity of Walleye Sander vitreus populations in northern Wisconsin foreshadows an underlying change in naturally reproduced juvenile Walleye survival. We used long-term stock and recruitment data from lakes in the Ceded Territory of Wisconsin to quantify density-dependent trends in juvenile Walleye survival and tested for the prevalence of depensation using the q parameter of Liermann and Hilborn (1997). Of 82 Walleye populations evaluated, about half exhibited depensatory recruitment. An analysis of the global q for all populations examined suggested that the posterior probability of depensatory dynamics was about 0.89. In addition, there were few clear cases of compensation—most populations exhibited weak density dependence. The general lack of strong compensatory recruitment across Walleye populations could leave these stocks vulnerable to stressors and unresponsive to rehabilitation. We present multiple lines of evidence to suggest that depensation is a plausible phenomenon explaining declines in Walleye populations in the Ceded Territory of Wisconsin and may be implicated in other invisible collapses of freshwater fisheries.
Respiratory‐swallow coordination and swallowing impairment in head and neck cancer
Authors: Hopkins‐Rossabi Theresa , Armeson Kent E. , Zecker Steven G. , Martin‐Harris Bonnie 
Published: January 2021

AbstractBackgroundThe aim of the study was to determine the link between frequency of optimal respiratory‐swallow coordination, swallowing impairment, and airway invasion in head and neck cancer (HNC) patients.MethodA cross‐sectional study of a heterogeneous group of HNC patients (49), precancer (N = 30) or postcancer treatment (N = 29), participated in a single Modified Barium Swallow Study (MBSS) with synchronized respiratory data.ResultsSpearman correlation coefficients revealed significant negative correlations between optimal respiratory‐swallow phase pattern and objective measures of swallowing impairment: penetration‐aspiration scale max, pharyngeal total, and oral total scores with Spearman correlation coefficients of −0.53 (z .001), −0.50 (P < .001), and −0.43 (P = .002), respectively. Optimal respiratory‐swallow pattern was significantly decreased (P = .03) in patients after cancer treatment compared with another patient group before cancer treatment.ConclusionThese findings indicate that as the percentage of optimal respiratory‐swallow phase patterns increase, swallowing impairment decreases in the HNC patient population.
Safety of switching to brexpiprazole in Japanese patients with schizophrenia: A post‐hoc analysis of a long‐term open‐label study
Authors: Ishigooka Jun , Inada Ken , Niidome Kazunari , Aoki Kazuo , Kojima Yoshitsugu , Iwashita Shuichi , Yamada Sakiko 
Published: January 2021

AbstractObjectivesTo determine the long‐term safety of switching to brexpiprazole from aripiprazole or non‐aripiprazole dopamine antagonists.MethodsPost‐hoc analysis of 56‐week study of Japanese outpatients with schizophrenia switched to brexpiprazole 2 mg/day over 4‐week switching period with further titration (1–4 mg/day) allowed during the 52‐week, open‐label period. Major assessment items: total/low‐density lipoprotein (LDL)‐/high‐density lipoprotein (HDL)‐cholesterol, triglycerides, blood glucose, body weight and prolactin. Secondary evaluations were related to efficacy, treatment emergent adverse events (TEAEs), extrapyramidal symptoms, and corrected QT interval (QTc).Results84/186 (45.2%) patients (aripiprazole, 32.9%; non‐aripiprazole, 54.8%) discontinued treatment over 56 weeks mainly because of consent withdrawal/adverse events. From baseline to Week 56, both groups showed minimal mean changes in total/LDL‐/HDL‐cholesterol, triglycerides, and glucose levels and a slight increase in mean (SD) body weight (aripiprazole, 1.1 [4.4] kg; non‐aripiprazole, 0.4 [4.6] kg). Mean prolactin levels increased slightly in the aripiprazole group, but decreased in the non‐aripiprazole group. Symptom severity scores decreased similarly in both groups. TEAEs occurred in 161/186 (86.6%) patients (aripiprazole, 84.1% [serious, 9.8%]; non‐aripiprazole, 88.5% [serious, 14.4%]). Few changes occurred in extrapyramidal symptom scales or QTc interval.ConclusionsSwitching to brexpiprazole is associated with a low long‐term risk for metabolic abnormalities (including weight gain), hyperprolactinemia, extrapyramidal symptoms and QTc changes and minimal changes in psychiatric symptoms.
Response of soil aggregate disintegration to the different content of organic carbon and its fractions during splash erosion
Authors: Li Hairu , Liu Gang , Gu Ju , Chen Hong , Shi Hongqiang , Abd Elbasit Mohamed A. M. , Hu Feinan 
Published: January 2021

AbstractAggregate disintegration is a critical process in soil splash erosion. However, the effect of soil organic carbon (SOC) and its fractions on soil aggregates disintegration is still not clear. In this study, five soils with similar clay contents and different contents of SOC have been used. The effects of slaking and mechanical striking on splash erosion were distinguished by using deionized water and 95% ethanol as raindrops. The simulated rainfall experiments were carried out in four heights (0.5, 1.0, 1.5 and 2.0 m). The result indicated that the soil aggregate stability increased with the increases of SOC and light fraction organic carbon (LFOC). The relative slaking and the mechanical striking index increased with the decreases of SOC and LFOC. The reduction of macroaggregates in eroded soil gradually decreased with the increase of SOC and LFOC, especially in alcohol test. The amount of macroaggregates (>0.25 mm) in deionized water tests were significantly less than that in alcohol tests under the same rainfall heights. The contribution of slaking to splash erosion increased with the decrease of heavy fractions organic carbon. The contribution of mechanical striking was dominant when the rainfall kinetic energy increased to a range of threshold between 9 J m−2 mm−1 and 12 m−2 mm−1. This study could provide the scientific basis for deeply understanding the mechanism of soil aggregates disintegration and splash erosion.
Long‐term database of Beasley Lake Watershed with 25 years of agricultural conservation practices
Authors: Lizotte Jr Richard E. , Yasarer Lindsey M. W. , Griffith Mark K. , Locke Martin A. , Bingner Ronald L. 
Published: January 2021

AbstractBeasley Lake Watershed is an agriculturally influenced drainage basin in western Mississippi that has been intensively studied for 25 years. As part of the USDA Conservation Effects Assessment Project (CEAP), the watershed has archived hydrology, precipitation, and water quality data in order to measure the effects of multiple USDA Natural Resources Conservation Service conservation practices on lake water quality. The long‐term database is available to researchers using a web‐based application, Sustaining the Earth's Watersheds, Agricultural Research Data System (STEWARDS). STEWARDS is a GIS‐based data retrieval application that encompasses spatial and temporal data collected from multiple sites within the watershed. This data note describes information located in the STEWARDS Beasley Lake Watershed database, including hydrology, precipitation, and water quality data. This information is valuable to researchers and agencies beyond the USDA as an available and useful database to improve the understanding of how land‐use practices affect the water quality of shallow lake systems.
Long‐run co‐variability between oil prices and economic policy uncertainty
Authors: Shahbaz Muhammad , Sharif Arshian , Belaid Fateh , Vo Xuan Vinh 
Published: January 2021

AbstractThis study investigates the long run co‐variability between prices of oil and uncertainty of economic policy by using monthly data for the period of 1998M1‐2018M12. We develop an innovative approach based on long‐run co‐variability approach developed recently by Muller and Watson (Müller & Watson, 2018). Our empirical results reveal the positive long‐run relationship between oil prices and uncertainty of economic policy for Australia, India, and Hong Kong whereas, a negative relationship is found between both variables in Brazil, China, Europe, Japan, Korea, Mexico, Russia, Sweden, and United States. We also find an insignificant effect of oil prices on uncertainty of economic policy in Chile, Colombia, Greece, Ireland, Netherland, and Singapore. This study extends the recent empirical findings on oil prices‐economic policy uncertainty, which imply a differential influence of oil prices on uncertainty economic policy in sampled countries. From policy perspectives, results of this paper are helpful to policy makers with long‐term concerns. They may guide the policy makers to identify the suitable policies to deal with substantial economic policy uncertainty and large oil prices shakes. In addition, they offer possibilities for drawing on the empirical evidence to deepen their understanding of dynamic relationship among oil prices and uncertainty of economic policy.
A simple way to novel 2‐dichloromethyl‐5‐aryl‐2H‐tetrazoles
Authors: Mikolaichuk Olga V. , Batyrenko Alina A. , Kornyakov Ilya V. , Trifonov Rostislav E. 
Published: January 2021

AbstractA series of 2‐dichloromethyl‐5‐aryl‐2H‐tetrazoles (3a–i) were synthesized by the reaction of 5‐aryl‐NH‐tetrazoles with trichloromethane in strong organic basic condition. The reaction represents a single‐stage access to a new class of promising compounds, which not only can have biological activity, but can also serve as intermediates for further alkylation of halogen and the introduction of various fragments into the structure. The compounds obtained were fully characterized by means of ESI–MS, 1H and 13C{1H} NMR spectroscopies, as well as by single‐crystal X‐ray diffraction (for 3b) the structural parameters as well as the vibrational corrections to enthalpies and total energies of the investigated systems were calculated using DFT B3LYP/6‐31+G** approach.