bluuebunny/crossref_metadata_2025_split huggingface dataset:
De novo deoxyribonucleotide biosynthesis regulates cell growth and tumor progression in small-cell lung carcinoma
Authors: Maruyama Ami , Sato Yuzo , Nakayama Joji , Murai Junko , Ishikawa Takamasa , Soga Tomoyoshi , Makinoshima Hideki 
Published: June 2021

AbstractDeoxyribonucleotide biosynthesis from ribonucleotides supports the growth of active cancer cells by producing building blocks for DNA. Although ribonucleotide reductase (RNR) is known to catalyze the rate-limiting step of de novo deoxyribonucleotide triphosphate (dNTP) synthesis, the biological function of the RNR large subunit (RRM1) in small-cell lung carcinoma (SCLC) remains unclear. In this study, we established siRNA-transfected SCLC cell lines to investigate the anticancer effect of silencing RRM1 gene expression. We found that RRM1 is required for the full growth of SCLC cells both in vitro and in vivo. In particular, the deletion of RRM1 induced a DNA damage response in SCLC cells and decreased the number of cells with S phase cell cycle arrest. We also elucidated the overall changes in the metabolic profile of SCLC cells caused by RRM1 deletion. Together, our findings reveal a relationship between the deoxyribonucleotide biosynthesis axis and key metabolic changes in SCLC, which may indicate a possible link between tumor growth and the regulation of deoxyribonucleotide metabolism in SCLC.
Reproducibility of automated habenula segmentation via deep learning in major depressive disorder and normal controls with 7 Tesla MRI
Authors: Lim Sang-Heon , Yoon Jihyun , Kim Young Jae , Kang Chang-Ki , Cho Seo-Eun , Kim Kwang Gi , Kang Seung-Gul 
Published: June 2021

AbstractThe habenula is one of the most important brain regions for investigating the etiology of psychiatric diseases such as major depressive disorder (MDD). However, the habenula is challenging to delineate with the naked human eye in brain imaging due to its low contrast and tiny size, and the manual segmentation results vary greatly depending on the observer. Therefore, there is a great need for automatic quantitative analytic methods of the habenula for psychiatric research purposes. Here we propose an automated segmentation and volume estimation method for the habenula in 7 Tesla magnetic resonance imaging based on a deep learning-based semantic segmentation network. The proposed method, using the data of 69 participants (33 patients with MDD and 36 normal controls), achieved an average precision, recall, and dice similarity coefficient of 0.869, 0.865, and 0.852, respectively, in the automated segmentation task. Moreover, the intra-class correlation coefficient reached 0.870 in the volume estimation task. This study demonstrates that this deep learning-based method can provide accurate and quantitative analytic results of the habenula. By providing rapid and quantitative information on the habenula, we expect our proposed method will aid future psychiatric disease studies.
Genetic diversity of Colletotrichum lupini and its virulence on white and Andean lupin
Authors: Alkemade J. A. , Messmer M. M. , Voegele R. T. , Finckh M. R. , Hohmann P. 
Published: June 2021

AbstractLupin cultivation worldwide is threatened by anthracnose, a destructive disease caused by the seed- and air-borne fungal pathogen Colletotrichum lupini. In this study we explored the intraspecific diversity of 39 C. lupini isolates collected from different lupin cultivating regions around the world, and representative isolates were screened for their pathogenicity and virulence on white and Andean lupin. Multi-locus phylogeny and morphological characterizations showed intraspecific diversity to be greater than previously shown, distinguishing a total of six genetic groups and ten distinct morphotypes. Highest diversity was found across South America, indicating it as the center of origin of C. lupini. The isolates that correspond to the current pandemic belong to a genetic and morphological uniform group, were globally widespread, and showed high virulence on tested white and Andean lupin accessions. Isolates belonging to the other five genetic groups were mostly found locally and showed distinct virulence patterns. Two highly virulent strains were shown to overcome resistance of advanced white lupin breeding material. This stresses the need to be careful with international seed transports in order to prevent spread of currently confined but potentially highly virulent strains. This study improves our understanding of the diversity, phylogeography and pathogenicity of a member of one of the world’s top 10 plant pathogen genera, providing valuable information for breeding programs and future disease management.
Younger age of onset and uveitis associated with HLA-B27 and delayed diagnosis in Thai patients with axial spondyloarthritis
Authors: Limsakul Naphruet , Chiowchanwisawakit Praveena , Permpikul Parichart , Thanaketpaisarn Yubolrat 
Published: June 2021

AbstractTo identify characteristics associated with HLA-B27, and to identify factors associated with delayed diagnosis in Thai patients with axial spondyloarthritis (axSpA). This cross-sectional study included Thai patients were diagnosed with axSpA by a rheumatologist at Siriraj Hospital. Clinical data were collected. Regression analyses were employed to identify factors associated with study outcomes. Of total 177 patients, 127 (72%) were positive HLA-B27. Uveitis [Odds ratio (OR) 4.0], age at onset of the first musculoskeletal symptom of ≤ 28 years [OR 3.5], female [OR 0.4], and psoriasis [OR 0.4] were significantly associated with HLA-B27 in multiple regression analysis. Those with positive HLA-B27 had less spinal flexibility. Elevated C-reactive protein (p = 0.012) was associated with shorter delay in diagnosis, while uveitis (p < 0.001) and younger age at onset of the first symptom (p = 0.002) were associated with longer delay in diagnosis in multiple regression analysis. Younger age at onset of the first musculoskeletal symptom and uveitis were associated with HLA-B27 and delayed diagnosis in axSpA patients. Young people with musculoskeletal symptom and uveitis should be referred to a rheumatologist to rule out or make a timely diagnosis of axSpA.
Radiomics is feasible for prediction of spread through air spaces in patients with nonsmall cell lung cancer
Authors: Onozato Yuki , Nakajima Takahiro , Yokota Hajime , Morimoto Jyunichi , Nishiyama Akira , Toyoda Takahide , Inage Terunaga , Tanaka Kazuhisa , Sakairi Yuichi , Suzuki Hidemi , Uno Takashi , Yoshino Ichiro 
Published: June 2021

AbstractTumor spread through air spaces (STAS) in non-small-cell lung cancer (NSCLC) is known to influence a poor patient outcome, even in patients presenting with early-stage disease. However, the pre-operative diagnosis of STAS remains challenging. With the progress of radiomics-based analyses several attempts have been made to predict STAS based on radiological findings. In the present study, patients with NSCLC which is located peripherally and tumors ≤ 2 cm in size on computed tomography (CT) that were potential candidates for sublobar resection were enrolled in this study. The radiologic features of the targeted tumors on thin-section CT were extracted using the PyRadiomics v3.0 software package, and a predictive model for STAS was built using the t-test and XGBoost. Thirty-five out of 226 patients had a STAS histology. The predictive model of STAS indicated an area under the receiver-operator characteristic curve (AUC) of 0.77. There was no significant difference in the overall survival (OS) for lobectomy between the predicted-STAS (+) and (−) groups (p = 0.19), but an unfavorable OS for sublobar resection was indicated in the predicted-STAS (+) group (p < 0.01). These results suggest that radiomics with machine-learning helped to develop a favorable model of STAS (+) NSCLC, which might be useful for the proper selection of candidates who should undergo sublobar resection.
Transmission of bee-like vibrations in buzz-pollinated plants with different stamen architectures
Authors: Nevard Lucy , Russell Avery L. , Foord Karl , Vallejo-Marín Mario 
Published: June 2021

AbstractIn buzz-pollinated plants, bees apply thoracic vibrations to the flower, causing pollen release from anthers, often through apical pores. Bees grasp one or more anthers with their mandibles, and vibrations are transmitted to this focal anther(s), adjacent anthers, and the whole flower. Pollen release depends on anther vibration, and thus it should be affected by vibration transmission through flowers with distinct morphologies, as found among buzz-pollinated taxa. We compare vibration transmission between focal and non-focal anthers in four species with contrasting stamen architectures: Cyclamen persicum, Exacum affine, Solanum dulcamara and S. houstonii. We used a mechanical transducer to apply bee-like vibrations to focal anthers, measuring the vibration frequency and displacement amplitude at focal and non-focal anther tips simultaneously using high-speed video analysis (6000 frames per second). In flowers in which anthers are tightly arranged (C. persicum and S. dulcamara), vibrations in focal and non-focal anthers are indistinguishable in both frequency and displacement amplitude. In contrast, flowers with loosely arranged anthers (E. affine) including those with differentiated stamens (heterantherous S. houstonii), show the same frequency but higher displacement amplitude in non-focal anthers compared to focal anthers. We suggest that stamen architecture modulates vibration transmission, potentially affecting pollen release and bee behaviour.
High stretch induces endothelial dysfunction accompanied by oxidative stress and actin remodeling in human saphenous vein endothelial cells
Authors: Girão-Silva T. , Fonseca-Alaniz M. H. , Ribeiro-Silva J. C. , Lee J. , Patil N. P. , Dallan L. A. , Baker A. B. , Harmsen M. C. , Krieger J. E. , Miyakawa A. A. 
Published: June 2021

AbstractThe rate of the remodeling of the arterialized saphenous vein conduit limits the outcomes of coronary artery bypass graft surgery (CABG), which may be influenced by endothelial dysfunction. We tested the hypothesis that high stretch (HS) induces human saphenous vein endothelial cell (hSVEC) dysfunction and examined candidate underlying mechanisms. Our results showed that in vitro HS reduces NO bioavailability, increases inflammatory adhesion molecule expression (E-selectin and VCAM1) and THP-1 cell adhesion. HS decreases F-actin in hSVECs, but not in human arterial endothelial cells, and is accompanied by G-actin and cofilin’s nuclear shuttling and increased reactive oxidative species (ROS). Pre-treatment with the broad-acting antioxidant N-acetylcysteine (NAC) supported this observation and diminished stretch-induced actin remodeling and inflammatory adhesive molecule expression. Altogether, we provide evidence that increased oxidative stress and actin cytoskeleton remodeling play a role in HS-induced saphenous vein endothelial cell dysfunction, which may contribute to predisposing saphenous vein graft to failure.
Phylogenomics illuminates the evolution of bobtail and bottletail squid (order Sepiolida)
Authors: Sanchez Gustavo , Fernández-Álvarez Fernando Á. , Taite Morag , Sugimoto Chikatoshi , Jolly Jeffrey , Simakov Oleg , Marlétaz Ferdinand , Allcock Louise , Rokhsar Daniel S. 
Published: June 2021

AbstractBobtail and bottletail squid are small cephalopods with striking anti-predatory defensive mechanisms, bioluminescence, and complex morphology; that inhabit nektobenthic and pelagic environments around the world’s oceans. Yet, the evolution and diversification of these animals remain unclear. Here, we used shallow genome sequencing of thirty-two bobtail and bottletail squids to estimate their evolutionary relationships and divergence time. Our phylogenetic analyses show that each of Sepiadariidae, Sepiolidae, and the three subfamilies of the Sepiolidae are monophyletic. We found that the ancestor of the Sepiolinae very likely possessed a bilobed light organ with bacteriogenic luminescence. Sepiolinae forms a sister group to Rossinae and Heteroteuthinae, and split into Indo-Pacific and Atlantic-Mediterranean lineages. The origin of these lineages coincides with the end of the Tethys Sea and the separation of these regions during the Eocene and the beginning of the Oligocene. We demonstrated that sepiolids radiated after the Late Cretaceous and that major biogeographic events might have shaped their distribution and speciation.
Accounting for tropical cyclones more than doubles the global population exposed to low-probability coastal flooding
Authors: Dullaart Job C. M. , Muis Sanne , Bloemendaal Nadia , Chertova Maria V. , Couasnon Anaïs , Aerts Jeroen C. J. H. 
Published: June 2021

AbstractStorm surges that occur along low-lying, densely populated coastlines can leave devastating societal, economical, and ecological impacts. To protect coastal communities from flooding, return periods of storm tides, defined as the combination of the surge and tide, must be accurately evaluated. Here we present storm tide return periods using a novel integration of two modelling techniques. For surges induced by extratropical cyclones, we use a 38-year time series based on the ERA5 climate reanalysis. For surges induced by tropical cyclones, we use synthetic tropical cyclones from the STORM dataset representing 10,000 years under current climate conditions. Tropical and extratropical cyclone surge levels are probabilistically combined with tidal levels, and return periods are computed empirically. We estimate that 78 million people are exposed to a 1 in 1000-year flood caused by extratropical cyclones, which more than doubles to 192 M people when taking tropical cyclones into account. Our results show that previous studies have underestimated the global exposure to low-probability coastal flooding by 31%.
Educational Challenges During the Pandemic for Students Who Have Hearing Loss
Authors: Schafer Erin C. , Dunn Andrea , Lavi Alexandra 
Published: June 2021

Purpose
The COVID-19 pandemic introduced new educational challenges for students, teachers, and caregivers due to the changed and varied learning environments, use of face masks, and social distancing requirements. These challenges are particularly pronounced for students with hearing loss who often require specific accommodations to allow for equal access to the curriculum. The purpose of this study was to document the potential difficulties that students with hearing loss faced during the pandemic and to generate recommendations to promote learning and engagement based on findings.

Method
A qualitative survey was designed to document the frequency of various learning situations (i.e., in person, remote virtual, and blended), examine the accessibility of technology and course content, and quantify hearing issues associated with safety measures and technology use in school-age students with hearing loss. Survey questions were informed from key educational issues reported in published articles and guidelines. The survey was completed by 416 educational personnel who work with students with hearing loss.

Results
Respondents indicated that most of their schools were providing remote or blended (in-person and remote) learning consisting of synchronous and asynchronous learning. Common accommodations for students with hearing loss were only provided some of the time with the exception of sign language interpreters, which were provided for almost all students who required them. According to the respondents, both students and caregivers reported issues or discomfort with the technology required for remote learning.

Conclusion
To ensure that students with hearing loss are provided equal access to the curriculum, additional accommodations should be considered to address issues arising from pandemic-related changes to school and learning practices including closed captioning, transcripts/notes, recordings of lectures, sign language interpreters, student check-ins, and family-directed resources to assist with technology issues.
Observation and calibration strategies for large-scale multi-beam velocity-resolved mapping of the [CII] emission in the Orion molecular cloud
Authors: Higgins R. , Kabanovic S. , Pabst C. , Teyssier D. , Goicoechea J. R. , Berne O. , Chambers E. , Wolfire M. , Suri S. T. , Buchbender C. , Okada Y. , Mertens M. , Parikka A. , Aladro R. , Richter H. , Güsten R. , Stutzki J. , Tielens A. G. G. M. 
Published: June 2021

Context.The [CII] 158 μm far-infrared fine-structure line is one of the dominant cooling lines of the star-forming interstellar medium. Hence [CII] emission originates in and thus can be used to trace a range of ISM processes. Velocity-resolved large-scale mapping of [CII] in star-forming regions provides a unique perspective of the kinematics of these regions and their interactions with the exciting source of radiation.Aims.We explore the scientific applications of large-scale mapping of velocity-resolved [CII] observations. With the [CII] observations, we investigate the effect of stellar feedback on the ISM. We present the details of observation, calibration, and data reduction using a heterodyne array receiver mounted on an airborne observatory.Methods.A 1.15 square degree velocity-resolved map of the Orion molecular cloud centred on the bar region was observed using the German REceiver for Astronomy at Terahertz Frequencies (upGREAT) heterodyne receiver flying on board the Stratospheric Observatory for Infrared Astronomy. The data were acquired using the 14 pixels of the German REceiver for Astronomy at Terahertz Frequencies that were observed in an on-the-fly mapping mode. 2.4 million spectra were taken in total. These spectra were gridded into a three-dimensional cube with a spatial resolution of 14.1 arcseconds and a spectral resolution of 0.3 km s−1.Results.A square-degree [CII] map with a spectral resolution of 0.3 km s−1is presented. The scientific potential of this data is summarized with discussion of mechanical and radiative stellar feedback, filament tracing using [CII], [CII] opacity effects, [CII] and carbon recombination lines, and [CII] interaction with the large molecular cloud. The data quality and calibration is discussed in detail, and new techniques are presented to mitigate the effects of unavoidable instrument deficiencies (e.g. baseline stability) and thus to improve the data quality. A comparison with a smaller [CII] map taken with theHerschel/Heterodyne Instrument for the Far-Infrared spectrometer is presented.Conclusions.Large-scale [CII] mapping provides new insight into the kinematics of the ISM. The interaction between massive stars and the ISM is probed through [CII] observations. Spectrally resolving the [CII] emission is necessary to probe the microphysics induced by the feedback of massive stars. We show that certain heterodyne instrument data quality issues can be resolved using a spline-based technique, and better data correction routines allow for more efficient observing strategies.
Vasopressoren: Physiologie, Pharmakologie und klinischer Einsatz
Authors: Keller Marius , Magunia Harry 
Published: June 2021

ZusammenfassungVasopressoren kommen besonders in kritischen, nicht selten lebensbedrohlichen Situationen der Anästhesie, Notfall- und Intensivmedizin zum Einsatz. Die Wahl der geeigneten Substanz sowie die Erkennung und Beherrschung möglicher Nebenwirkungen sind von großer Bedeutung. Im folgenden Beitrag sollen daher die klinisch wichtigsten Substanzen vorgestellt und deren pharmakologische Charakteristika beleuchtet werden.
Natürliche und synthetische Katecholamine
Authors: Lehmann Martin , Rossaint Jan 
Published: June 2021

ZusammenfassungKatecholamine sind aus dem anästhesiologischen Alltag nicht mehr wegzudenken. Ob nun während einer Sectio bei gesunden jungen Frauen, im Operationssaal, bei multimorbiden Patienten auf Intensivstation oder im Notfalleinsatz auf der Straße: Das notwendige Basiswissen, um Katecholamine korrekt anzuwenden, ist entscheidend für das Outcome unserer Patienten und steht im Fokus dieses Beitrags.
Individualised Homeopathic Treatment of Acne—An Analysis of 83 Patients
Authors: Nwabudike Lawrence Chukwudi 
Published: June 2021

Abstract
Background Acne is a common disorder of the pilosebaceous follicle. The face, back and chest are usually involved. It leads to significant diminution in quality of life. Numerous treatments are documented in therapeutic guidelines. Naturopathic approaches have been proposed in some, but the role of homeopathy is not examined.
Methods In this study, 83 patients treated for acne with individualised homeopathic medicine alone were reviewed. Most had received conventional acne treatment, with limited success prior to presentation for homeopathy. Each patient was prescribed a single homeopathic medicine and followed up at 6- to 8-week intervals. The individualisation process resulted in 17 different medicines being used in this group. Photographic documentation was obtained per patient, with informed consent. Patients were classified as mild (comedonal acne with no papules or pustules), moderate (inflammatory and non-inflammatory lesions) and severe (predominantly inflammatory lesions: pustules, cysts, nodules). Results of treatment were recorded as remission (decrease in new lesion number, duration and intensity), failure to respond, and lost to follow-up (LTF).
Results The average age of patients was 21.5 years (range 11–45 years). The F:M ratio was 55 (66.3%):28 (33.7%). Average pre-treatment duration was 5.5 years (0.25–22 years). Seven (8.4%) patients had mild acne, 37 (44.6%) moderate, and 39 (47%) severe acne. There were 13 (15.7%) LTFs, two (2.4%) failed to respond, and 68 (81.9%) went into remission. Average time to remission was 1.9 months (range 1.5–6 months), with no relapses or side-effects. The most commonly prescribed medicines were Lycopodium (38.6%), Palladium (15.7%) and Platinum (12.1%).
Conclusion Individualised homeopathy may be useful for acne therapy. The most useful medicines appeared to be Lycopodium, Palladium and Platinum, though 17 different medicines were used in this study, underscoring the value of individualisation of therapy, a key characteristic of homeopathy.
Dynamics of artistic style: a computational analysis of the Maker’s motoric qualities in a clay-relief practice
Authors: Dick Nir , Prusak Ayala , Zoran Amit Raphael 
Published: June 2021

AbstractThe artistic style has been extensively discussed within diverse perspectives, usually studying the physical qualities of existing artifacts as the resource for investigation. This paper proposes a novel analysis of the dynamics of artistic style, as represented by a set of motor features, techniques, and their temporal interplay. The researchers hypothesize that unique characteristics of individuals’ styles are represented as transitions between motor activities, which would allow for computational analysis of style. As a case study, the researchers tracked a carving knife used in a clay-relief technique in two studies, one comprising (i) twelve sessions and five novice participants; and the other (ii) twenty-eight sessions with a single skilled artist. The analysis reveals that dynamic style is (i) unique and consistent in novices’ creative processes and that (ii) different subcategories of making can be observed in an experienced participant related to the subject of the work. These offer the possibility of quantitatively studying the making process irrespective of the esthetic qualities of the finished artifact, which allows for diverse computational applications.