bluuebunny/crossref_metadata_2025_split huggingface dataset:
The First Years of DNA Sequencing
Authors: Arslan Mevlüt 
Published: December 2024

We were able to sequence DNA molecules a 100 years later, after its discovery. Wu was the first scientist to report DNA sequencing in 1968. In 1970, we learned the first eight-base sequence of lambda DNA from Wu’s studies, which were based on synthesis by DNA polymerase to be sequenced DNA. In 1971, a four-base sequence was added also, and we learned a twelve-base sequence in total. In the same year, Englund reported a direct DNA sequencing method that allowed in vivo DNA to be sequenced directly without synthesis. After 1971, DNA sequencing studies have developed in two ways: sequencing by synthesis and direct sequencing. Sanger reported the first DNA sequencing study in 1973, when Wu introduced the primer-extension method to sequence-specific DNA regions. Although Wu’s and other scientists’ studies were very valuable, the techniques were difficult. Sanger’s methods described in 1973 and 1975 were also difficult. Practical methods were highly demanded. In 1977, Sanger introduced a more practical chain termination method, also known as the “dideoxy method,” which was related to sequencing by synthesis. Direct sequencing of in vivo DNA was highly desired. In the rout, Maxam-Gilberts introduced the chemical-cleavage method in 1977. Seven years after the first DNA sequencing, DNA sequencing methods reached a significant milestone.
Influence of Literacy Ability to The Interest of Reading and Critical Thinking Ability in Indonesia
Authors: Hakim Farrel Ariella 
Published: December 2024

Literacy must become a culture that is instilled from an early age so as to produce competent people who are able to understand, involve, use, analyze and transform existing information. This can make a person able to recognize and develop self-potential as an effort to improve self-quality so that they are able to compete with the outside world, to be able to improve this literacy ability, individuals must make literacy a culture that is instilled from an early age so as to produce competent people who are able to understand, surround, use, analyze, and transform existing information. This can make a person able to recognize and develop self-potential as an effort to improve self-quality so that they are able to compete, with good literacy skills, it is hoped that the ability to think critically will also increase.
Türkiye Cumhuriyet Merkez Bankası’nın Yayınladığı Sektör Bilançoları Üzerinden İnşaat Sektörünün Finansal Oranlarının Analizi
Authors: Aydın Engin 
Published: December 2024

İnşaat sektörü konut üretiminin yanı sıra okul hastane kamu binaları (bina inşaatı) ile doğalgaz boru hatları, barajlar, karayolları, havalimanları gibi altyapı projelerinin (bina dışı inşaatı yapıların inşaatı) üretimini kapsamaktadır. Bu sektör ekonomik büyümeye, istihdama, ileri ve geri bağlantılı sektörlere katkısı nedeniyle Türkiye gibi gelişmekte olan ülkelerde stratejik öneme sahiptir. Bu kapsamda hem inşaat sektörünün genel olarak hem de bina inşaatı ve bina dışı yapıların inşaatı alt sektörleri bazında finansal performanslarının yıllar itibariyle değerlendirilmesi sektörün durumunu ve gidişatını görmek için gereklidir.
Çalışmada inşaat sektörünün performansın ölçülmesi için finansal tablolar analizi kapsamında en çok kullanılan yöntem olan oran analizi yöntemi kullanılmıştır. Bu kapsamda inşaat sektörünün iki alt sektörü olan bina inşaatı ve bina dışı yapıların inşaatı alt sektörlerinin ölçek bazında (mikro, küçük, orta ve büyük) Türkiye Cumhuriyet Merkez Bankası tarafından yayınlanmış 2021-2023 yılları arasındaki konsolide edilmiş mali tablolarından yararlanılarak finansal oranlar hesaplanmıştır. Çalışma kapsamında inşaat sektörünün; kısa vadeli borç ödeme gücünün ölçülmesinde likidite oranları, finansal yapısının analizi için finansal yapı oranları, varlıkların verimli kullanılıp kullanılmadığının analizi için faaliyet oranları, karlılığın analizi için karlılık oranları kullanılmıştır. Böylece 2021-2023 yılları arasında inşaat sektörünün önemli iki alt sektörü olan bina inşaatı ve bina dışı yapıların inşaatı alt sektörleri hem birbirleriyle hem ölçek bazında hem de yıllar itibarı ile karşılaştırılarak inşaat sektörünün finansal performansı ölçülmüş, yorumlanmış ve değerlendirilmiştir.
Dolgu Barajların Göçmesi ve İçsel Erozyon Mekanizması
Authors: Topçu Sadettin , Savaş Hasan , Tosun Hasan 
Published: December 2024

Son yıllarda baraj göçme vakaları ve sonuçları tüm dünyada ilgi çeken felaketler arasına girmiştir. Rezervuarlarında biriktirdikleri milyonlarca metreküp suyun ani bir şekilde sellenmesiyle mansap bölgelerinde yer alan yerleşim alanlarında can ve mal kayıplarına yol açan baraj göçme vakaları, afet kapsamında değerlendirilen bir konu haline gelmeye başlamıştır. Ülkelerin altyapı yatırımlarını tehdit eden baraj göçmeleri, uzun yıllar boyunca yaşanan vakalardan tecrübe edinilerek elde edilen verilere dayalı bir konudur. Bu nedenledir ki özellikle yer yapısının taklit edilmesiyle tasarlanan dolgu barajların göçme nedenleri ve mekanizmaları oldukça fazla başlık halinde değerlendirilmektedir. Bu kitap bölümü çalışması kapsamında da dolgu barajların göçme nedenleri anlatılmış ve bununla birlikte dolgu barajların göçme nedenlerinin başında gelen içsel erozyon mekanizması detaylı bir şekilde sunulmuştur.
Dialog Antar Agama sebagai Implementasi Pendidikan Perdamaian
Authors: Nur Ashilla Fathya , Tari Natia , Rizki Akbar Muhammad 
Published: December 2024

Dialog antar agama memainkan peran penting dalam mempromosikan perdamaian dan pemahaman dalam masyarakat yang beragam. Studi ini mengkaji pelaksanaan dialog antaragama sebagai komponen kunci pendidikan perdamaian. Dengan menggunakan pendekatan tinjauan literatur yang komprehensif, penelitian ini menggali peran dialog antaragama dalam implementasi pendidikan perdamaian. Program Halaqah Damai muncul sebagai langkah konkret untuk memupuk toleransi, mengurai stigma, dan membina masyarakat yang harmonis dan inklusif. Dengan mendorong dan memanfaatkan dialog sebagai alat penghubung di antara individu-individu dari berbagai agama, inisiatif pendidikan perdamaian bertujuan untuk menumbuhkan rasa saling pengertian dan kerja sama di antara pendudukIndonesia yang beragam.
Семиотика коммуникации в цифровом арт-пространстве Казахстана (на материале выставок городов Астаны и Алматы)
Authors: Нурбосынова Балжан 
Published: December 2024

This study explores the semiotics of communication on Kazakhstani digital visual art platforms in Astana and Almaty. Empirical analysis of exhibitions visited in spring and summer 2024 supports the theoretical framework of semiotic communication used in this work. The article aims to conduct a semiotic analysis of communication processes at Kazakhstani digital visual art exhibitions and assess the significance of artistic signs in cultural information exchange. Based on Yuri Lotman’s concept of communication semiotics, the study hypothesizes that cultural communication in the digital era activates the semantic, syntactic, and pragmatic content of visual art images. The empirical part focuses on museum exhibitions, multimedia projects, and works of digital artists, examining information exchange among the viewer, artist, and artwork. It identifies and classifies the types of signs in communication, offering interpretations from a semantic perspective. Observations demonstrate the dynamics of art communications using digital technologies. The author notes the expansion of the semantic range in contemporary Kazakhstani art and the efforts of digital artists and curators to combine traditional art forms with digital technologies (syntactics of artistic construction). The article shows a shift in communication pragmatics, revealing a trend toward activating the artist’s civic engagement. The value of this material lies in illustrating positive processes in contemporary Kazakhstani art. However, the author highlights issues such as insufficient public awareness of digital art content, lack of platforms uniting digital artists’ works, and the absence of open dialogue venues for critical analysis of new digital visual art.
การประยุกต์แนวความคิดมาใช้กับการออกแบบผลิตภัณฑ์กระเป๋าสุภาพสตรี
Authors: เรืองถฐาวร บุญญคมสัน 
Published: December 2024

การประยุกต์แนวความคิดมาใช้กับการออกแบบผลิตภัณฑ์กระเป๋าสุภาพสตรีนี้มีวัตถุประสงค์เพื่อประยุกต์ใช้แนวคิดการออกแบบในการสร้างสรรค์กระเป๋าสุภาพสตรีที่สวยงามและมีคุณค่า โดยเน้นการใช้สัดส่วนทองและหลักการองค์ประกอบศิลป์ กระบวนการประกอบด้วยหลายขั้นตอนดังนี้

การศึกษาทฤษฎีและแนวคิด การรวบรวมและวิเคราะห์ทฤษฎีที่เกี่ยวข้องกับสัดส่วนทองและองค์ประกอบศิลป์
การวิเคราะห์ตัวอย่างกระเป๋า การศึกษารูปแบบ ขนาดสัดส่วน รูปทรง และลวดลายของกระเป๋าสุภาพสตรีที่มีอยู่ในตลาดจำนวน 7 ชนิด ได้แก่ 1) กระเป๋าเป้สะพายหลัง 2) กระเป๋าถือ 3) กระเป๋าสะพายข้างเพื่อเก็บเอกสาร 4) กระเป๋าแบบใช้สะพายไหล่ 5) กระเป๋าสำหรับเดินเล่น 6) กระเป๋าทรงถุง 7) กระเป๋าทรงพระจันทร์เสี้ยว
การถอดรหัสแนวคิดในการออกแบบกระเป๋า ตามแนวคิดและทฤษฎี สไตล์ เทรนด์ เป็นตัวอย่างการทดลองให้เห็นกระบวนการออกแบบนำมาสู่ ความสวยงาม ความสมมาตร ความน่าดึงดูดใจ ที่เกิดจากการถอดรหัสแนวคิด

ผลการศึกษาแสดงให้เห็นว่า การประยุกต์ใช้สัดส่วนทองและหลักการองค์ประกอบศิลป์ เป็นแนวคิดที่นำมาใช้เป็นแนวทางในการออกแบบผลิตภัณฑ์กระเป๋าสุภาพสตรี ตรงตามความต้องการของผู้บริโภคและตลาด กระบวนการนี้ช่วยให้นักออกแบบสามารถนำมาประยุกต์ใช้สำหรับสร้างสรรค์ผลิตภัณฑ์ที่มีคุณภาพและให้เกิดมิติในการจัดวาง ความกว้าง ความสูง ความลึก หูหิ้ว รูปร่าง รูปทรง จะเน้นไปที่ความสมมาตร มีความโดดเด่น สร้างคุณค่า มีรูปแบบที่สวยงาม มีสไตล์ ทำให้ผลงานการออกแบบผลิตภัณฑ์กระเป๋าสุภาพสตรีตรงตามจุดประสงค์ของบุคลิกกระเป๋าที่แตกต่างกัน
THE INFLUENCE OF ECONOMIC VALUE ADDED, GOING CONCERN OPINION AND DIVIDEND POLICY ON STOCK PRICES IS MEASURED BY MARKET VALUE ADDED
Authors: Anastasia Awa  , Endang Ruhiyat  , Sugiyanto  
Published: December 2024

This study aims to examine the influence of Economic Value Added, Going Concern Opinion and Dividend Policy on Stock Prices as analyzed by Market Value Added. This type of research is associative quantitative research. The type of data used is secondary data obtained from the company's www.idx.co.id and website. The population in this study is manufacturing companies in the consumer non-cyclicals sector that have been listed on the IDX for the 2018-2022 period. The determination of the sample used the purposive sampling method, the number of research samples was 29 companies, with a research year of 5 years, the total data in this study was 145 data and the data was tested using the help of EViews software version 12. The data analysis technique used is panel data model regression. Based on the results of this study, the Economic Value Added variable has a positive effect on the Stock Price, the Going Concern Opinion has no effect on the Stock Price, the Dividend Policy has no effect on the Stock Price, the Market Value Added cannot decode the relationship between the Economic Value Added and the Stock Price, the Market Value Added cannot decode the relationship of Going Concern Opinion to Stock Price, Market Value Added cannot decode the relationship of Dividend Policy to Stock Price.
Socialization of Goleman's 6 Leadership Types to Prepare Young Leaders for the Future
Authors: Yulyantika Nadya Rindy , Permata Fedina Dyah Ayu , Romadlona Nohan Arum 
Published: December 2024

This leadership is the art of influencing someone, either individually or in groups. According to Daniel Goleman, leadership styles are divided into 6: Commanding, Visionary, Affiliative, Democratic, Pacesetting, and Coaching. Basically, leadership can be formed and trained since school age. This is done so that when they are faced with a problem, they can easily find several alternative solutions to the problem In order to realize a young generation that has a leader's soul, community service activities are carried out at SMP Ma'arif Kota Batu. This socialization activity aims to introduce and increase knowledge about Goleman's 6 leadership styles. The method used in socialization is using the lecture method using power point media with material on 6 types of leadership according to Goleman. To measure the level of knowledge of students, a pre test was conducted before socialization and a post test was conducted after socialization. The result is an increase in knowledge of female students after socialization so that it is expected that students can recognize their own leadership style. As for the suggestions in this activity, namely the use of leadership material to direct female students in increasing their understanding and leadership skills in an organization.
EXPLORING THE PREDICTIVE ASSOCIATION OF BIG FIVE PERSONALITY TRAITS WITH PSYCHOLOGICAL DISTRESS AMONG UNDERGRADUATE UNIVERSITY STUDENTS
Authors: Assma Ashraf  , Amreen  , Muneeba Shakil  
Published: December 2024

The objective of this study was to assess the predictive association among the big five personality traits and psychological distress among undergraduate students. The study also explored the frequency of Psychological Distress among undergraduate students. The hypothesis of the study were a) big five personality traits would predict the level of psychological distress among undergraduate students. b) there would be a higher prevalence of psychological distress among undergraduate students c) the level of psychological distress would be higher among female undergraduate students as compared to their male counterparts. The sample of the study consisted of 480 participants within the age range of 18-24 years from various departments of the University of Karachi. Participants were selected using purposive-convenient sampling technique. The measures used in the study were demographic form, big five inventory (John et.al, 2008) and the depression anxiety stress scale (Lovibond & Lovibond, 1995). With reference to the frequency of mental health problems, 52.70%, 67.70% and 40.62% undergraduate university students reported moderate to extreme level of depression, anxiety and stress, respectively. Findings are reflective of significant differences between male (M=45.5, SD= 22.8) and female (M=49.8, SD= 29.7) undergraduate students on the domain of Psychological distress (t (477) = 1.959, p< .05) showing that the level of psychological distress is higher in female students as compared to male students. Regarding the predictive association among big five personality traits and psychological distress, findings reflect a significant predictive impact of agreeableness (R2= .028, F=13.7, P<.001), conscientiousness (R2= .034, F=16.7, P<.001) and neuroticism (R2= .158, F=89.8, P<.001) on psychological distress among undergraduate students. In addition agreeableness and conscientiousness were found to be negative predictors causing 2.8 % and 3.4 % variance in the scores of psychological distress respectively. Conversely, neuroticism was found to be a positive predictor of psychological distress among undergraduate students causing 15.8 % variation. However extraversion (R2= .006, F=13.7, P>.001) and openness to experience (R2= .000, F=.000, P>.001) were found to be insignificant predictors of psychological distress among undergraduate students. The finding of the study highlights the importance of personality traits and its impact on the psychological well-being of students. These finding can be utilized for effective treatment planning in the domain of student counseling.
Regulation of lymphoma in vitro by CLP36 through the PI3K/AKT/CREB signaling pathway
Authors: Lv Chao , Chen Guannan , Lv Shuang 
Published: December 2024

Background
CLP36 is also known as PDZ and LIM Domain 1 (PDLIM1) that is a ubiquitously-expressed α-actinin-binding cytoskeletal protein involved in carcinogenesis, and our current study aims to explore its involvement in lymphoma.

Methods
Accordingly, the CLP36 expression pattern in lymphoma and its association with the overall survival was predicted. Then, qPCR was applied to gauge CLP36 expression in lymphoma cells and determine the knockdown efficiency. The survival, proliferation and apoptosis of CLP36-silencing lymphoma cells were tested. Cell viability, proliferation and apoptosis were assessed based on cell counting kit-8 (CCK-8) assay, colony formation assay, EdU staining, and flow cytometry, respectively. Additionally, qPCR was used to calculate the expressions of proteins associated with metastasis and apoptosis, while immunoblotting was employed to determine the phosphorylation status of phosphoinositide 3-kinase (PI3K)/protein kinase B (AKT)/cAMP-response element binding protein (CREB).

Results
CLP36 expression was relatively higher in lymphoma, which was associated with a poor prognosis. Also, CLP36 was highly-expressed in lymphoma cells and the silencing of CLP36 contributed to the suppressed survival and proliferation as well as the enhanced apoptosis of lymphoma cells. Further, CLP36 silencing repressed the expressions of Cadherin 2 (CDH2) and Vimentin (VIM) yet promoted those of Bax and Caspase 3 in lymphoma cells, concurrent with the reduction on the phosphorylation of PI3K, AKT and CREB, all of which were confirmed to be positively correlated with CLP36.

Conclusion
This study, so far as we are concerned, provided evidence on the involvement of CLP36/PI3K/AKT/CREB axis in lymphoma, which may be contributive for the identification on the relevant molecular targets of lymphoma.
Boron‐Doped Polycyclic Aromatic Hydrocarbons (B‐PAHs): Synthesis, Properties, and Applications
Authors: Lorenzo‐García María M. , Fasano Francesco , Bonifazi Davide 
Published: September 2021

AbstractInspired by the doping approach used in silicon technology to building inorganic semiconductors, in this chapter we tackle the emerging approach in organic synthesis of exploiting the replacement of carbon atoms with isolobal heteroatoms. Capitalizing on the idea for which the insertion of heteroatoms of graphitic nanostructure preserves the planar geometry of the π‐scaffold, one could envisage to tune the optoelectronic properties of π‐conjugated molecular materials by the only insertion of heteroatoms. Herein, we discuss all B‐containing rings that can be envisaged to construct doped π‐conjugated structures integrating a graphenoid framework. In the first section, we guide the reader through the synthesis, properties, and applications of the most relevant examples of B‐doped PAHs, in which one or more sp2‐hybridized carbon atoms have been replaced by B atoms through the formation of the CB bond. Past and recent synthetic developments of aromatic and nonaromatic B‐doped ring, i.e., boracycle, are discussed. B‐PAHs are amenable to be classified by ring size of the boracycle, that is, three‐, four‐, five‐, six‐, or seven‐membered rings. In a second avenue, we focused the attention on the use of the boron–nitrogen (BN) pair site as isoelectronic and isostructural doping units replacing CC bonds in PAHs. While three‐ and four‐membered rings containing BN couples are known but not extensively explored, five‐ and six‐membered rings are certainly the most studied structural π‐conjugated motifs. In the last section, the heart of the discussion is centered on B‐PAHs containing BO bonds, mainly in five‐ and six‐membered rings.
Strategic Engineering of Sub‐5 nm Dyes@CDs Nanoassemblies Platform for Super Resolution Imaging
Authors: Man Zhongwei , Lv Zheng , Xu Zhenzhen , Yao Jiannian , Fu Hongbing 
Published: September 2021

AbstractThe design and construction of fluorescence probes with small size, high quantum yield, photostability, and solubility in an aqueous solution to achieve biomarker have become a focus of interest in super‐resolution bioimaging application. Herein, four kinds of GFP‐like supramolecular nanoassemblies (SNAs) are constructed in a very facile manner by the host–guest interaction between isopercolic acid derivatives (IADs) and cyclodextrin (CD). 1H NMR, Fourier transform infrared spectroscopy (FT‐IR) and single crystal structure well prove the formation of SNAs. Moreover, compared to the initial state, the fluorescence quantum yield of the SNAs is dramatically improved through suppressing nonradiative relaxation pathways and shielding quenchers. The bimolecular exciton–exciton annihilation (bmEA) process under the high pumping fluence has been suppressed effectively. Both the improved gain and the reduced loss facilitate the buildup of stimulated emission and enable the high stimulated emission efficiency of these SNAs. Sub‐5 nm IADs@β‐CDs‐mediated STED imaging exhibits excellent stimulated emission depletion (STED) performance: high photostability, low saturation intensity (≈0.50 MW cm−2), and high resolution (30 nm) under PSTED‐775 nm of 109 MW cm−2. This supramolecular strategy avoids the tedious molecular synthesis process and paves a new pathway for insights into building up novel high‐performance STED probes.
Mitochondrion‐Anchored Photosensitizer with Near Infrared‐I Aggregation‐Induced Emission for Near Infrared‐II Two‐Photon Photodynamic Therapy
Authors: He Zhenyan , Gao Yuting , Zhang Huimin , Xue Ying , Meng Fanling , Luo Liang 
Published: September 2021

AbstractTwo‐photon photodynamic therapy (2P‐PDT) that employs photosensitizers (PSs) with 2P absorption is particularly intriguing in cancer treatment, in that 2P excitation enables precise spatial localization and deep tissue penetration. Here, a donor‐π‐acceptor PS (named TPBPy) with near infrared (NIR) aggregation‐induced emission (AIE) is designed and synthesized for imaging‐guided 2P‐PDT. The maximal photoluminescence (PL) peak of TPBPy is as high as 720 nm when it is encapsulated in liposomes. Upon 2P irradiation by a laser in NIR‐II window (λ = 1000 nm), TPBPy exhibits strong NIR‐I PL in a multicellular tumor spheroids (MCTSs) model, showing an imaging depth of 210 µm that is significantly higher than upon one‐photon irradiation. Moreover, TPBPy localizes specifically on mitochondrion, an important organelle in cell oxidative metabolism and apoptosis. When exposed to the NIR‐II irradiation, TPBPy can efficiently generate singlet oxygen (1O2) and trigger cell death. The efficacy of TPBPy‐mediated 2P‐PDT has also been validated using 4T1 tumor mouse model, the growth of which is significantly suppressed upon NIR‐II laser irradiation. TPBPy herein serves as an excellent candidate to suppress deep tumor tissues through NIR‐II 2P‐PDT, and also renders a new paradigm to construct mitochondrion‐anchored AIE luminogens for future cancer theranostic applications.