bluuebunny/crossref_metadata_2025_split huggingface dataset:
PENGARUH JUMLAH ULIR DAN KEKASARAN PERMUKAAN TERHADAP KINERJA TURBIN ULIR MIKRO HIDRO
Authors: Putra Adi , Joditiadhi Andi , Qiram Ikhwanul 
Published: June 2021

Pemanfaatan energi potensial air salah satunya adalah menggunakan teknologi turbin ulir sebagai pembangkit listrik mikrohidro. Beberapa parameter yang mempengaruhi kinerja turbin ulir adalah jumlah ulir, kekasaran permukaan,sudut kemiringan poros dan debit aliran. Penelitian ini bertujuan untuk mengetahui pengaruh jumlah ulir dan kekasaran permukaan terhadap kinerja turbin ulir. Penelitian dilakukan dengan eksperimen, dimana turbin menggunakan variasi jumlah ulir 7, 9, 11. Kekasaran menggunakan variasi grid 0 (tanpa kekasaran), grid 40, grid 60. Sudut kemiringan poros menggunakan 30⁰, 33⁰, 36⁰ dan debit aliran menggunakan bukaan valve 50⁰ (502,14), 70⁰ (22,42), (90⁰) 803,52. Analisis kinerja turbin ditinjau berdasarkan kecepatan putaran (Rpm) dan tegangan (volt) yang dihasilkan. Hasil penelitian menunjukkan debit aliran, jumlah dan sudut turbin serta kekasaran permukaan sudu mempengaruhi unjuk kerja turbin ulir. Semakin rendah jumlah ulir dan tingkat kekasaran maka putaran dan tegangan akan semakin meningkat dan sebaliknya. Jumlah sudu dan tingkat kekasaran permukaan sudu akan memicu peningkatan luas hambatan aliran fluida. Hasil pengujian menunjukkan kinerja turbin terbaik pada variasi 7 ulir, dengan sudut kemiringan 33o dengan kekasaran grid 0 (tanpa kekasaran) menghasilkan tegangan 7,26 volt pada 2928,40 rpm.
Issues of therapy and prevention of post-inflammatory acne pigmentation
Authors: Kruglova L. S. , Gryazeva N. V. , Bondarenko V. V. 
Published: June 2021

Azelaic acid is well suited for the treatment of acne in women, where PIH occurs most often (it is not an obstacle for use during pregnancy), the drug may be combined with systemic and topical therapy, may combined with skin care and decorative cosmetics, has no restrictions when used in active sun, possesses light peeling effect.Material and methods.The study evaluated the effectiveness of the combined method, including systemic isotretinoin and Skinaren®cream 20 %, in patients with moderate acne – group 1A (n = 24) in comparison with isotretinoin monotherapy – group 1B (n = 25). The efficacy of Skinoren®20 % cream was also studied in patients with post-inflammatory hyperpigmentation – the group 2 (n = 37).Research results.The combination of 20 % azelaic acid cream (Skinoren®) with oral isotretinoin (from 4th months of admission) in patients with adult acne allows to influence the formation of post-inflammatory hyperpigmentation, which ultimately leads to a more significant aesthetic result (88 % with the achievement of IGA0) and contributes to an improvement in the quality of life (reduction of APSEA by 87 %) The safety of the 20 % azelaic acid cream (Skinoren®) is assessed as very high, which is confirmed by the absence of adverse events in most patients. The use of 20 % azelaic acid cream (Skinoren®) as a monotherapy for post-inflammatory hyperpigmentation causes a pronounced positive effect in stopping PIH in all patients, while achieving clear skin is noted in 78.4 %.
SMARCC1 Suppresses Tumor Progression by Inhibiting the PI3K/AKT Signaling Pathway in Prostate Cancer
Authors: Xiao Zhao-Ming , Lv Dao-Jun , Yu Yu-zhong , Wang Chong , Xie Tao , Wang Tao , Song Xian-Lu , Zhao Shan-Chao 
Published: June 2021

BackgroundSWI/SNF-related, matrix-associated, actin-dependent regulator of chromatin subfamily C member 1 (SMARCC1) protein is a potential tumor suppressor in various cancers. However, its role in prostate cancer (PCa) remains controversial. The aim of this study was to determine the biological function of SMARCC1 in PCa and explore the underlying regulatory mechanisms.MethodsThe expression of SMARCC1 was validated in PCa tissues by immunohistochemistry. Meanwhile, function experiments were used to evaluate the regulatory role on cell proliferation and metastasis in PCa cells with SMARCC1 depletion both in vitro and in vivo. The expression levels of relevant proteins were detected by Western blotting.ResultsOur finding showed that SMARCC1 was significantly downregulated in prostate adenocarcinoma, with a higher Gleason score (GS) than that in low GS. The decreased expression of SMARCC1 was significantly correlated with a higher GS and poor prognosis. Additionally, we found that silencing of SMARCC1 dramatically accelerated cell proliferation by promoting cell cycle progression and enhancing cell migration by inducing epithelial mesenchymal transition (EMT). Furthermore, depletion of SMARCC1 facilitated PCa xenograft growth and lung metastasis in murine models. Mechanistically, the loss of SMARCC1 activated the PI3K/AKT pathway in PCa cells.ConclusionSMARCC1 suppresses PCa cell proliferation and metastasis via the PI3K/AKT signaling pathway and is a novel therapeutic target.
Combining Hyperbranched and Linear Structures in Solid Polymer Electrolytes to Enhance Mechanical Properties and Room-Temperature Ion Transport
Authors: Jing Benxin , Wang Xiaofeng , Shi Yi , Zhu Yingxi , Gao Haifeng , Fullerton-Shirey Susan K. 
Published: June 2021

Polyethylene oxide (PEO)-based polymers are commonly studied for use as a solid polymer electrolyte for rechargeable Li-ion batteries; however, simultaneously achieving sufficient mechanical integrity and ionic conductivity has been a challenge. To address this problem, a customized polymer architecture is demonstrated wherein PEO bottle-brush arms are hyperbranched into a star architecture and then functionalized with end-grafted, linear PEO chains. The hierarchical architecture is designed to minimize crystallinity and therefore enhance ion transport via hyperbranching, while simultaneously addressing the need for mechanical integrity via the grafting of long, PEO chains (Mn = 10,000). The polymers are doped with lithium bis(trifluoromethane) sulfonimide (LiTFSI), creating hierarchically hyperbranched (HB) solid polymer electrolytes. Compared to electrolytes prepared with linear PEO of equivalent molecular weight, the HB PEO electrolytes increase the room temperature ionic conductivity from ∼2.5 × 10–6 to 2.5 × 10−5 S/cm. The conductivity increases by an additional 50% by increasing the block length of the linear PEO in the bottle brush arms from Mn = 1,000 to 2,000. The mechanical properties are improved by end-grafting linear PEO (Mn = 10,000) onto the terminal groups of the HB PEO bottle-brush. Specifically, the Young’s modulus increases by two orders of magnitude to a level comparable to commercial PEO films, while only reducing the conductivity by 50% below the HB electrolyte without grafted PEO. This study addresses the trade-off between ion conductivity and mechanical properties, and shows that while significant improvements can be made to the mechanical properties with hierarchical grafting of long, linear chains, only modest gains are made in the room temperature conductivity.
Machine Learning With GA Optimization to Model the Agricultural Soil-Landscape of Germany: An Approach Involving Soil Functional Types With Their Multivariate Parameter Distributions Along the Depth Profile
Authors: Ließ Mareike , Gebauer Anika , Don Axel 
Published: June 2021

Societal demands on soil functionality in agricultural soil-landscapes are confronted with yield losses and environmental impact. Soil functional information at national scale is required to address these challenges. On behalf of the well-known theory that soils and their site-specific characteristics are the product of the interaction of the soil-forming factors, pedometricians seek to model the soil-landscape relationship using machine learning. Following the rationale that similarity in soils is reflected by similarity in landscape characteristics, we defined soil functional types (SFTs) which were projected into space by machine learning. Each SFT is described by a multivariate soil parameter distribution along its depth profile. SFTs were derived by employing multivariate similarity analysis on the dataset of the Agricultural Soil Inventory. Soil profiles were compared on behalf of differing sets of soil properties considering the top 100 and 200 cm, respectively. Various depth weighting coefficients were tested to attribute topsoil properties higher importance. Support vector machine (SVM) models were then trained employing optimization with a distributed multiple-population hybrid Genetic algorithm for parameter tuning. Model training, tuning, and evaluation were implemented in a nested k-fold cross-validation approach to avoid overfitting. With regards to the SFTs, organic soils were differentiated from mineral soils of various particle size distributions being partly influenced by waterlogging and groundwater. Further SFTs reflect soils with a depth limitation within the top 100 cm and high stone content. Altogether, with SVM predictive model accuracies between 0.7 and 0.9, the agricultural soil-landscape of Germany was represented with eight SFTs. Soil functionality with regards to the soil’s capacity to store plant-available water and soil organic carbon is well characterized. Four additional soil functions are described to a certain extent. An extension of the approach to fully cover soil functions such as nutrient cycling, agricultural biomass production, filtering of contaminants, and soil as a habitat for soil biota is possible with the inclusion of additional soil properties. Altogether, the developed data product represents the 3D multivariate soil parameter space. Its agglomerated simplicity into a limited number of spatially allocated process units provides the basis to run agricultural process models at national scale (Germany).
Challenging Disease Ontology by Instances of Atypical PKHD1 and PKD1 Genetics
Authors: de Fallois Jonathan , Schönauer Ria , Münch Johannes , Nagel Mato , Popp Bernt , Halbritter Jan 
Published: June 2021

BackgroundAutosomal polycystic kidney disease is distinguished into dominant (ADPKD) and recessive (ARPKD) inheritance usually caused by either monoallelic (PKD1/PKD2) or biallelic (PKHD1) germline variation. Clinical presentations are genotype-dependent ranging from fetal demise to mild chronic kidney disease (CKD) in adults. Additionally, exemptions from dominant and recessive inheritance have been reported in both disorders resulting in respective phenocopies. Here, we comparatively report three young adults with microcystic-hyperechogenic kidney morphology based on unexpected genetic alterations beyond typical inheritance.MethodsNext-generation sequencing (NGS)-based gene panel analysis and multiplex ligation-dependent probe amplification (MLPA) of PKD-associated genes, familial segregation analysis, and reverse phenotyping.ResultsThree unrelated individuals presented in late adolescence for differential diagnosis of incidental microcystic-hyperechogenic kidneys with preserved kidney and liver function. Upon genetic analysis, we identified a homozygous hypomorphic PKHD1 missense variant causing pseudodominant inheritance in a family, a large monoallelic PKDH1-deletion with atypical transmission, and biallelic PKD1 missense hypomorphs with recessive inheritance.ConclusionBy this report, we illustrate clinical presentations associated with atypical PKD-gene alterations beyond traditional modes of inheritance. Large monoallelic PKHD1-alterations as well as biallelic hypomorphs of both PKD1 and PKHD1 may lead to mild CKD in the absence of prominent macrocyst formation and functional liver impairment. The long-term renal prognosis throughout life, however, remains undetermined. Increased detection of atypical inheritance challenges our current thinking of disease ontology not only in PKD but also in Mendelian disorders in general.
Efficacy and Safety of Bone Marrow-Derived Mesenchymal Stem Cells for Chronic Antibody-Mediated Rejection After Kidney Transplantation- A Single-Arm, Two-Dosing-Regimen, Phase I/II Study
Authors: Wei Yongcheng , Chen Xiaoyong , Zhang Huanxi , Su Qun , Peng Yanwen , Fu Qian , Li Jun , Gao Yifang , Li Xirui , Yang Shicong , Ye Qianyu , Huang Huiting , Deng Ronghai , Li Gang , Xu Bowen , Wu Chenglin , Wang Jiali , Zhang Xiaoran , Su Xiaojun , Liu Longshan , Xiang Andy Peng , Wang Changxi 
Published: June 2021

ObjectiveTo investigate the efficacy and safety of bone marrow-derived mesenchymal stem cells (BM-MSCs) on chronic active antibody-mediated rejection (cABMR) in the kidney allograft.MethodsKidney recipients with biopsy-proven cABMR were treated with allogeneic third-party BM-MSCs in this open-label, single-arm, single-center, two-dosing-regimen phase I/II clinical trial. In Regimen 1 (n=8), BM-MSCs were administered intravenously at a dose of 1.0×106 cells/kg monthly for four consecutive months, while in Regimen 2 (n=15), the BM-MSCs dose was 1.0×106 cells/kg weekly during four consecutive weeks. The primary endpoints were the absolute change of estimated glomerular filtration rate (eGFR) from baseline (delta eGFR) and the incidence of adverse events associated with BM-MSCs administration 24 months after the treatment. Contemporaneous cABMR patients who did not receive BM-MSCs were retrospectively analyzed as the control group (n =30).ResultsTwenty-three recipients with cABMR received BM-MSCs. The median delta eGFR of the total BM-MSCs treated patients was -4.3 ml/min per 1.73m2 (interquartile range, IQR -11.2 to 1.2) 2 years after BM-MSCs treatment (P=0.0233). The median delta maximum donor-specific antibody (maxDSA) was -4310 (IQR -9187 to 1129) at 2 years (P=0.0040). The median delta eGFR of the control group was -12.7 ml/min per 1.73 m2 (IQR -22.2 to -3.5) 2 years after the diagnosis, which was greater than that of the BM-MSCs treated group (P=0.0342). The incidence of hepatic enzyme elevation, BK polyomaviruses (BKV) infection, cytomegalovirus (CMV) infection was 17.4%, 17.4%, 8.7%, respectively. There was no fever, anaphylaxis, phlebitis or venous thrombosis, cardiovascular complications, or malignancy after BM-MSCs administration. Flow cytometry analysis showed a significant decreasing trend of CD27-IgD- double negative B cells subsets and trend towards the increase of CD3+CD4+PD-1+/lymphocyte population after MSCs therapy. Multiplex analysis found TNF-α, CXCL10, CCL4, CCL11 and RANTES decreased after MSCs treatment.ConclusionKidney allograft recipients with cABMR are tolerable to BM-MSCs. Immunosuppressive drugs combined with intravenous BM-MSCs can delay the deterioration of allograft function, probably by decreasing DSA level and reducing DSA-induced injury. The underlying mechanism may involve immunomodulatory effect of MSCs on peripheral B and T cells subsets.
Mapping T Cell Responses to Native and Neo-Islet Antigen Epitopes in at Risk and Type 1 Diabetes Subjects
Authors: Arif Sefina , Pujol-Autonell Irma , Kamra Yogesh , Williams Evangelia , Yusuf Norkhairin , Domingo-Vila Clara , Shahrabi Yasaman , Pollock Emily , Khatri Leena , Peakman Mark , Tree Timothy , Lorenc Anna 
Published: June 2021

AimsRecent studies highlight the potentially important role of neoepitopes in breaking immune tolerance in type 1 diabetes. T cell reactivity to these neoepitopes has been reported, but how this response compares quantitatively and phenotypically with previous reports on native epitopes is not known. Thus, an understanding of the relationship between native and neoepitopes and their role as tolerance breakers or disease drivers in type 1 diabetes is required. We set out to compare T cell reactivity and phenotype against a panel of neo- and native islet autoantigenic epitopes to examine how this relates to stages of type 1 diabetes development.MethodsFifty-four subjects comprising patients with T1D, and autoantibody-positive unaffected family members were tested against a panel of neo- and native epitopes by ELISPOT (IFN-γ, IL-10, and IL-17). A further subset of two patients was analyzed by Single Cell Immune Profiling (RNAseq and TCR α/β) after stimulation with pools of native and neoepitope peptides.ResultsT cell responses to native and neoepitopes were present in patients with type 1 diabetes and at-risk subjects, and overall, there were no significant differences in the frequency, magnitude, or phenotype between the two sets of peptide stimuli. Single cell RNAseq on responder T cells revealed a similar profile in T1D patients stimulated with either neo- or native epitopes. A pro-inflammatory gene expression profile (TNF-α, IFN-γ) was dominant in both native and neoepitope stimulated T cells. TCRs with identical clonotypes were found in T cell responding to both native and neoepitopes.Conclusion/InterpretationThese data suggest that in peripheral blood, T cell responses to both native and neoepitopes are similar in terms of frequency and phenotype in patients with type 1 diabetes and high-risk unaffected family members. Furthermore, using a combination of transcriptomic and clonotypic analyses, albeit using a limited panel of peptides, we show that neoepitopes are comparable to native epitopes currently in use for immune-monitoring studies.
Screening and Validation of the Hypoxia-Related Signature of Evaluating Tumor Immune Microenvironment and Predicting Prognosis in Gastric Cancer
Authors: Pei Jun-Peng , Zhang Chun-Dong , Yusupu Maimaititusun , Zhang Cheng , Dai Dong-Qiu 
Published: June 2021

BackgroundHypoxia is one driving factor of gastric cancer. It causes a series of immunosuppressive processes and malignant cell responses, leading to a poor prognosis. It is clinically important to identify the molecular markers related to hypoxia.MethodsWe screened the prognostic markers related to hypoxia in The Cancer Genome Atlas database, and a risk score model was developed based on these markers. The relationships between the risk score and tumor immune microenvironment were investigated. An independent validation cohort from Gene Expression Omnibus was applied to validate the results. A nomogram of risk score model and clinicopathological factor was developed to individually predict the prognosis.ResultsWe developed a hypoxia risk score model based on SERPINE1 and EFNA3. Quantified real-time PCR was further applied to verified gene expressions of SERPINE1 and EFNA3 in gastric cancer patients and cell lines. A high-risk score is associated with a poor prognosis through the immunosuppressive microenvironment and immune escape mechanisms, including infiltration of immunosuppressive cells, expression of immune checkpoint molecules, and enrichment of signal pathways related to cancer and immunosuppression. The nomogram basing on the hypoxia-related risk score model showed a good ability to predict prognosis and high clinical net benefits.ConclusionsThe hypoxia risk score model revealed a close relationship between hypoxia and tumor immune microenvironment. The current study potentially provides new insights of how hypoxia affects the prognosis, and may provide a new therapeutic target for patients with gastric cancer.
Diversion Colitis and Probiotic Stimulation: Effects of Bowel Stimulation Prior to Ileostomy Closure
Authors: Rodríguez-Padilla Ángela , Morales-Martín Germán , Pérez-Quintero Rocío , Rada-Morgades Ricardo , Gómez-Salgado Juan , Ruiz-Frutos Carlos 
Published: June 2021

Background: Diversion colitis is a non-specific inflammation of a defunctionalised segment of the colon after a temporary stoma has been performed. This inflammation is associated with a change in the colonic flora.Aim: To evaluate the efficacy and safety of preoperative stimulation of the efferent loop with probiotics prior to closure of the protective ileostomy in patients operated on colorectal carcinoma and its effect on diversion colitis. A prospective, randomised, double-blind, controlled study is carried out.Methods: Patients who underwent surgery for colorectal carcinoma with protective ileostomy pending reconstructive surgery and with diversion colitis as diagnosis are included. Randomised and divided into two groups. Histological and endoscopic changes were evaluated after stimulation, after restorative surgery and during the short-term follow-up after surgery.Results: Patients in CG were distributed according to the endoscopic index of severity in pre-stimulation/post-stimulation as follows: severe n = 9/9 (25.7%), moderate n = 23/23 (65.7%), and mild n = 3/3 (8.6%); compared to the distribution in SG, severe n = 9/0 (26.5/0%), moderate n = 23/3 (67.6/8.8%), mild n = 2/19 (5.9/55.9%) and normal colonoscopy in 0/12 patients (0/35.3%).Conclusion: Probiotic stimulation of the efferent loop is a safe and effective method, managing to reduce both macroscopic and microscopic colitis, as well as a decrease in symptoms in the short term after reconstructive surgery.
The Association Between Dietary Inflammatory Index and Parathyroid Hormone in Adults With/Without Chronic Kidney Disease
Authors: Qin Zheng , Yang Qinbo , Liao Ruoxi , Su Baihai 
Published: June 2021

Aims: We aimed to assess the association between dietary inflammation index (DII) with parathyroid hormone (PTH) and hyperparathyroidism (HP) in adults with/without chronic kidney disease (CKD).Methods: Data were obtained from the 2003–2006 National Health and Nutrition Examination Survey (NHANES). The participants who were &lt;18 years old, pregnant, or missing the data of DII, PTH, and CKD were excluded. DII was calculated based on a 24-h dietary recall interview for each participant. Weighted multivariable regression analysis and subgroup analysis were conducted to estimate the independent relationship between DII with PTH and the HP in the population with CKD/non-CKD.Results: A total of 7,679 participants were included with the median DII of −0.24 (−2.20 to 1.80) and a mean PTH level of 43.42 ± 23.21 pg/ml. The average PTH was 45.53 ± 26.63 pg/ml for the participants in the highest tertile group compared with 41.42 ± 19.74 pg/ml in the lowest tertile group (P &lt; 0.0001). The rate of HP was 11.15% overall, while the rate in the highest DII tertile was 13.28 and 8.60% in the lowest DII tertile (P &lt; 0.0001). The participants with CKD tended to have higher PTH levels compared with their counterparts (61.23 ± 45.62 vs. 41.80 ± 19.16 pg/ml, P &lt; 0.0001). A positive association between DII scores and PTH was observed (β = 0.46, 95% CI: 0.25, 0.66, P ≤ 0.0001), and higher DII was associated with an increased risk of HP (OR = 1.05, 95% CI: 1.02, 1.08, P = 0.0023). The results from subgroup analysis indicated that this association was similar in the participants with different renal function, gender, age, BMI, hypertension, and diabetes statuses and could also be appropriate for the population with CKD.Conclusions: Higher consumption of a pro-inflammatory diet appeared to cause a higher PTH level and an increased risk of HP. Anti-inflammatory dietary management may be beneficial to reduce the risk of HP both in the population with and without CKD.