bluuebunny/crossref_metadata_2025_split huggingface dataset:
Machine Learning based Identification and Classification of Disorders in human Knee Joint - Computational Approach
Authors: A. Balajee , Rajagopal Venkatesh 
Published: June 2021

Abstract
Earlier identification of knee joint pathology helps the therapist to provide the appropriate clinical procedures to control the deteriorating process of arthritis. Beyond usual medical investigations, computational techniques have been used for the diagnosis of knee joint disorder. Among different methodologies, Vibroarthrographic technique is employed to identify knee joint disorder. Machine Learning contains number of classification methods for the given data. A novel technique called Greedy sequential backward feature selection based Radial kernelized least square support vector classification (GSBFS-RKLSSVC) is introduced for accurate detection of knee joint pathology with minimum time. The proposed GSBFS-RKLSSVC technique consists of three processes namely feature selection, feature evaluation and classification. Initially number of VAG signal images are taken from the dataset for detection of knee joint disorder. The relevant feature is selected through the Greedy mutual informative regressed sequential backward selection algorithm to reduce an initial dimensional feature space into a low dimensional feature subspace. Following this the dichotomous logit regression is applied to select the best features and discard others. Therefore, the feature selection process of the proposed GSBFS-RKLSSVC minimizes the time consumption of the knee joint pathology detection. Once the signal features are extracted, RKLSSVC is applied to detect the normal and abnormal VAG signal. Decision boundary is utilized by the classifier to categorize the samples based on the similarity between the training features and testing features. As a result, the accurate classification is obtained with a minimum error rate. The observed result indicates that GSBFS-RKLSSVC achieves higher accuracy, sensitivity, specificity and reduces time than the conventional methods.
Aerobic Exercise Inhibits Inflammatory Response in Atherosclerosis via Sestrin1 Protein
Authors: sun yunfeng , Li ke , wu wei , yang keping 
Published: June 2021

Abstract
Background Aerobic exercise plays an important role in prevention and treatment of atherosclerosis but its role in inflammatory response is not completely clear. Inflammatory response is the main pathological process during occurrence and development stage of atherosclerosis. SESNs are considered as anti-inflammation protein in atherosclerosis. Results In current study, a high expression level of SESN1 is identified under the condition of aerobic exercise, further investigation shows levels of IL-1β/IL-6/TNF-α are significantly suppressed compared to those atherosclerosis mice with no aerobic training. Besides, we find that the activation of NK-κB signaling is impeded. Combine with our previous study, SESN1 is considered as the downstream factor of aerobic exercise which tend to inhibit the activation of inflammatory signaling and result in suppress the expression level of inflammatory factors. Another exciting finding is that MMP9/13 are also suppressed，but the potential mechanism is unclear. Conclusion Overall, present study sheds light on the significance of aerobic exercise for inflammation and stability of plaque through SESN1 may help developing new clinical treatments of atherosclerosis.
Renal Replacement Therapy for Patients Requiring Extracorporeal Membrane Oxygenation: A Multicenter International Survey
Authors: THY Michael , Augustin Pascal , Tran-Dinh Alexy , Montravers Philippe , de Tymowski Christian 
Published: June 2021

Abstract
ObjectivesPatients receiving extracorporeal membrane oxygenation (ECMO) often require renal replacement therapy (RRT). Insertion of a dialysis catheter may be challenging in these patients. Direct connection of RRT lines on an ECMO circuit (DCRE) may help circumvent this problem. However, no guidelines exist on this issue, and various types of practices may exist. This study aimed to describe the practice of RTT in patients on ECMO, including the direct connection of RTT lines on the ECMO circuit (DCRE) as well as the perception and concerns related to this technique.DesignInternational surveySetting.Worldwide diffusion via email.Subject.Professionals involved in the management of ECMOInterventions:Respondents’ characteristics were analyzed. Respondents always or often performing RRT via the ECMO circuit were classified in the ECMO group, and those using a dialysis catheter were classified in the DC group. The two groups were compared regarding their characteristics and their practice in ECMO and RRT. Participants were asked about their perceptions of DCRE and actual problems previously encountered. Measurements and Main Results:From March 2019 to October 2019, 298 participants answered the questionnaire from 46 different countries. Only 28% were working in pediatric departments. Among the 165 participants commonly performing RRT in patients on ECMO, 100 (61%) performed mainly RRT via the ECMO circuit, and 65 (39%) performed RRT via DC. Pediatric practice and a longer experience were the only noticeable characteristics of the ECMO group. The most reported fear regarding DCRE was the risk of air embolism (n=84, 28%), but the most encountered problem was unmanageable pressure alarms in RRT devices.ConclusionThe present study showed significant heterogeneity in RRT practices in patients on ECMO. The lower experience of the DC group, the high rates of fears toward DCRE, and pressure alarm issues suggested that protocols and training may overcome reluctance and technical difficulties. Further research may provide data to back specific guidelines. Take-home messageThe present study showed significant heterogeneity in RRT practices in patients on ECMO showing lower experience, fears and pressure alarm issues as barriers for direct connection of RRT lines on ECMO circuit, suggesting needs for protocols, training and specific guidelines.140-character TweetLow experience, fears and pressure alarm issues as barriers for direct connection of RRT lines on ECMO circuit, suggesting needs for protocols, training and specific guidelines.
Serum Homocysteine, Lipid Profile and BMI as Atherosclerotic Risk Factors in Children with Numerical Chromosomal Aberrations
Authors: Youness Eman R. , El-Daly Sherien M , Abdallah Hanaa Reyad , El-Bassyouni Hala T , Megahed Hisham , Khedr Azzah A , Elhady Marwa , Abuelhamid Walaa A 
Published: June 2021

Abstract
Children with chromosomal disorders are at greater risk for cardiovascular comorbidities. Dyslipidemia and homocysteinemia have been identified to play a role in the pathogenesis of premature atherosclerosis. The current study aimed to explore the association of serum homocysteine level in children with numerical chromosomal disorders (Klinefelter syndrome, Down syndrome, and Turner syndrome) and explore its relation to dyslipidemia among such children. This case control study included 60 children with numerical chromosomal disorders (18 Klinefelter syndrome 22 Down syndrome and 20 Turner syndrome) diagnosed by cytogenetic analysis. In addition, 37 healthy normal weight children were included as control group. Anthropometric assessment was done for all groups including weight, height and body mass index was calculated. Serum level of homocysteine, cholesterol, triglycerides, high density lipoprotein and low-density lipoprotein were measured for all included children. Serum homocysteine level was significantly higher in children with numerical chromosomal disorders in comparison to healthy controls. Serum homocysteine level has significant positive correlation with cholesterol and low-density lipoprotein. Despite of the significant positive correlation between homocysteine serum levels and body mass index (BMI) in children with numerical chromosomal disorders, there was no significant difference in homocysteine level between children with and without obesity. Regression analysis demonstrated significant association between homocysteinemia and LDL level in children with chromosomal disorders (odd ratio: 1.396; 95% confidence interval: 1.177–1.657). There is significant association between dyslipidemia and homocysteinemia in children with non-mosaic numerical chromosomal disorders that positively correlated with body mass index.
Paediatric Oncology Ward Nurses’ Experiences of Patients’ Deaths in China: A Qualitative Study
Authors: Ma Ruo Han , Zhao Xue Ping , Ni Zhi Hong , Xue Xiao Ling 
Published: June 2021

Abstract
Background: Considering cancer death is second only to accidental death in the number of lives claimed each year,nurses in paediatric oncology wards often experience helplessness, sadness, frustration and such other adverse emotions when they witness children’s death due to cancer.However,there is a lack of qualitative studies on nurses who witness the death of children in paediatric oncology wards in China. Method: Semi-structured interviews were conducted with nurses in the paediatric oncology wards at three children’s hospitals in Jiangsu,China,between January and June 2019.A total of 22 paediatric oncology ward nurses (18 female and 4male) aged between 26 and 39 years were enrolled. A previously developed and pilot-tested interview guide was used for the interviews. The number of interviews was determined by data saturation.Results: Nurses in paediatric oncology wards have strong stress responses to facing the death of children. They reported experiencing complex psychological feelings and have different coping attitudes. Nursing managers should pay attention to problems faced by nurses in paediatric oncology wards, and take targeted measures in terms of continuing training courses, improving the psychological adaptability of oncology professional nurses, and providing them substantive support.Conclusion: The findings of this study increase the knowledge and understanding regarding a seldom-studied topic in China. Healthcare authorities should recognise and understand the needs of paediatric oncology ward nurses,who often witness the death of children.Appropriate and effective support measures should be planned and implemented for these nurses to maintain their mental health, thus enabling them to better serve patients.
U2AF2 Promote the Proliferation and Migration of Esophageal Adenocarcinoma Cells by Sustaining the Mrna Stability of SNORA21
Authors: Lijuan Gao , Yongshun Chen , Bin Li 
Published: June 2021

Abstract
Background

Esophageal adenocarcinoma has gains predominance with squamous carcinoma moving to a second place in the western world. The 5-year survival rate of patients with esophageal cancer (EC) is still between 15–25%, although the treatment strategies for EC have been improved. RNA-binding proteins (RBPs) are key players in post-transcriptional events. Particularly, the U2 snRNP auxiliary (U2AF2), as a canonical RBP, has been reported to play an important role in the development, progression, and metastasis of several human cancers. However, the biological role of U2AF2 in the context of malignancies, including EC, has not yet been reported. This study is aimed to investigate the role and mechanism of U2AF2 in esophageal adenocarcinoma cell line.
Methods

The expression level of U2AF2 and SNORA21 was analysed by TCGA database. The expression level of U2AF2 and SNORA21 in esophageal adenocarcinoma cells was determined by Western Blot and RT-qPCR assay. The cell viability and proliferation was detected by CCK8 assay and BrdU incorporation assay. The apoptosis was examined by caspase3 immunocytofluorescense. The protein levels of Bax, Bcl-2 and E-cadherin were explored by Western Blot assay. The interaction between U2AF2 and SNORA21 was predicted by Starbase 3.0 and confirmed by RIP assay. The RNA regulated by U2AF2 was through RNA-seq analysis on the basis of downregulation of U2AF2.
Results

The expression of U2AF2 and SNORA21 was found to be upregulated and served as an unfavourable factor which negatively related with overall survival of esophageal adenocarcinoma patients. Silencing the expression of U2AF2 or SNORA21 could suppress the proliferation and migration of OE19 and OE33 cells and stimulate its apoptosis. Moreover, we identified that the U2AF2 could bind and interact with SNORA21 to sustain its stability. Furthermore, we show that overexpression of SNORA21 could rescue the proliferation inhibition phenotype caused by si-U2AF2.
Conclusion

Our study provides several lines of evidence supporting the intriguing concept that U2AF2 could promote the proliferation of OE19 and OE33 cells by directly targeting SNORA21, which might serve as a novel candidate biomarker and a new target for the treatment of esophageal adenocarcinoma.
SPECT Findings on Neuropsychiatric Symptoms Caused by Nitrous Oxide Abuse
Authors: Wang Li , Yin Lijie , Wang Qian , Wang Renbin , Liu Zunjing , Dong Mingrui , Duan Xiaohui , Zheng Yumin , Hong Wen , Liu Fang , Tie Changle 
Published: June 2021

Abstract
Objective To investigate the clinical, neuropsychological and changes of regional cerebral blood flow (rCBF) perfusion in patients with neuropsychiatric symptoms caused by nitrous oxide (N2O) abuse. Methods Sixteen patients with neuropsychiatric symptoms caused by nitrous oxide abuse were recruited. 25-30mci 99mTc-ECD was administered intravenously. the SPECT/CT images were collected with low-energy and high-resolution collimator. The region uptake statistics of different brain regions of interest between patients with N2O abuse and normal people of the same age group from background software database were calculated automatically. Results The clinical manifestations of the 16 patients with neuropsychiatric symptoms were mood lability, anxiety, hallucination, delusion, Agitated, Confusion, and other psychiatric symptoms. In addition, 15 patients of them also complained their memory decline. 14 patients manifested as numbness or paresthesias. 14 patients developed limb weakness, whose motor impairments were more severe in lower limbs than upper limbs. 8 patients also accompanied by urinary and defecation disturbance. Neuropsychological examination: BPRS score was 54.69±11.48, the score of HAMD was 30.00 ± 11.06, the score of HAMA was 18.06 ± 5.77, the score of MMSE was28.06 ± 2.29, and the score of MoCA was 25.06 ± 3.40. SPECT showed hypoperfusion in the frontal lobe and the temporal lobe, which consistent with the clinical findings. Conclusion It was the first study to demonstrate obvious effect of N2O abuse on CBF in patients with neuropsychiatric symptom. CBF perfusion imaging is helpful to detect the changes of local brain functional activity in patients with N2O abuse.
GPR120 Prevents Colorectal Adenocarcinoma Progression by Sustaining The Mucosal Barrier Integrity
Authors: Rubbino Federica , Garlatti Valentina , Garzarelli Valeria , Massimino Luca , Spanò Salvatore , Iadarola Paolo , Cagnone Maddalena , Giera Martin , Heijink Marieke , Guglielmetti Simone , Arena Vincenzo , Malesci Alberto , Laghi Luigi , Danese Silvio , Vetrano Stefania Stefania 
Published: June 2021

Abstract
Background&Aims: GPR120 (encoded by FFAR4 gene) is a receptor for long chain fatty acids, activated by ω-3 PUFAs, and expressed in many cell types. Its role in the context of colorectal cancer (CRC) is still puzzling with many controversial evidences. Here, we explored the involvement of epithelial GPR120 in the CRC development. Methods: Both in vitro and in vivo experiments were conducted to mimic the conditional deletion of the receptor from gut epithelium. Intestinal permeability and integrity of mucus layer were assessed by using Evans blue dye and immunofluorescence for MUC-2 protein, respectively. Microbiota composition, presence of lipid mediators and short chain fatty acids were analyzed in the stools of conditional GPR120 and wild type (WT) mice. Incidence and grade of tumors were evaluated in all groups of mice before and after colitis-associated cancer. Finally, GPR120 expression was analyzed in 9 human normal tissues, 9 adenomas, and 17 primary adenocarcinomas. Results: Our work for the first time highlight the role of the receptor in the progression of colorectal cancer. We observed that the loss of epithelial GPR120 in the gut results into increased intestinal permeability, microbiota translocation and dysbiosis, which turns into hyperproliferation of epithelial cells, likely through the activation of β -catenin signaling. Therefore, the loss of GPR120 represents an early event of CRC, but avoid its progression as invasive cancer. Conclusion: these results demonstrate that the epithelial GPR120 receptor is essential to maintain the mucosal barrier integrity and to prevent CRC developing. Therefore, our data pave the way to GPR120 as an useful marker for the phenotypic characterization of CRC lesions and as new potential target for CRC prevention.
High Quality-Factor Dual-Band Fano Resonances Induced by Bound States in The Continuum Using A Planar Nanohole Slab
Authors: Sang Tian , Mi Qing , Pei Yao , Yang Chaoyu , Li Shi , Wang Yueke , Ma Bin 
Published: June 2021

Abstract
In photonics, it is essential to achieve high quality (Q)-factor resonances to enhance light-mater interactions for improving performances of optical devices. Herein, we demonstrate that high Q-factor dual-band Fano resonances can be achieved by using a planar nanohole slab (PNS) based on the excitation of bound states in the continuum (BICs). By shrinking or expanding the tetramerized holes of the superlattice of the PNS, symmetry-protected BICs can be excited and the locations of Fano resonances as well as their Q-factors can be flexibly tuned. Physical mechanisms for the dual-band Fano resonances can be interpreted as the resonant couplings between the electric-toroidal dipoles or the magnetic-toroidal dipoles based on the far-field multiple decompositions and the near-field distributions of the superlattice. The dual-band Fano resonances of the PNS possess polarization independent feature, they can be survived even the geometric parameters of the PNS are significantly altered, making them more suitable for potential applications.
miR-218-5p in Endometrial Microenvironment Prevents the Migration of Ectopic Endometrial Stromal Cells by Inhibiting LASP1
Authors: Zhang Ziyu , Wang Yaoqing , Zeng Liqin , Yu Kaihui , Wang Yuanqin , Luo Yong , Liu Faying , Yang Bicheng , Zou Yang , Wang Liqun , Huang Ouping 
Published: June 2021

Abstract
Our previous two-dimensional electrophoresis experiments showed that the expression of LASP1 in patients with endometriosis was significantly higher than that of control endometrium. However, the molecular mechanism by which LASP1 is regulated in endometriosis/adenomyosis is unknown. Herein, qPCR was performed to analysis the expression levels of LASP1 and miR-218-5p between EMs cells and control cells. Immunofluorescence in situ hybridization was carried out to measure the expression level of miR-218-5p in ectopic endometrium versus normal endometrium. After miR-218-5p mimic or inhibitor were transfected, the transwell experiment was carried out to see the effect of miR-218-5p on the migration of ESCs. EdU was used to measure cell proliferation rate. Dual-luciferase reporter assay (DR) was used to verify the binding of hsa-miR-218-5p to the 3'UTR of LASP1. Western blot and immunofluorescence analysis were carried out to identify the protein expression pattern of LASP1 and EMT markers in endometrial tissue. Our results show that high expression of LASP1 is related to the development of EMs. miR-218-5p inhibits the expression level of LASP1 by binding the 3'UTR region of LASP1. miR-218-5p is negatively correlated with the expression of LASP1 protein in endometriosis cells and tissues. Without affecting proliferation, miR-218-5p can increase the migration of ESCs. miR-218-5p is mainly secreted from blood vessels and expressed in the muscle layer around the endometrium. miR-218 also inhibits mesenchymal transition of epithelial cells by inhibiting the expression of Vimentin.
The Image Presentation of Inflammatory Follicular Dendritic Cell Sarcoma Mimic Primary Cholangiocarcinoma in Epstein-Barr Virus Prevalent Country: A Case Report and Literature Review
Authors: Chao WuPo , Chen Yi-Chan , Yang Cheng-Han , Soong Rueyshyang 
Published: June 2021

Abstract
Background: Follicular dendritic cell sarcoma (FDCS) is a very rare malignant soft tissue tumor. About half of the cases occurred in lymph node, mostly around neck. The uncommon inflammatory variant of FDCS is an Epstein-Barr virus (EBV)-associated neoplasm. It was first introduced as Inflammatory pseudotumor-like FDC in 2001. Few cases had been found in liver and spleen as primary tumor. It mimicked the presentation of cholangiocarcinoma in this case and few had been reported as primary liver tumor yet. Case report: We represent a 53-year-old man who was referred to our department with a liver tumor. The tumor was accidently found during health exam at lateral segment, which was suspected to be cholangiocarcinoma on Magnetic Resonance Imaging(MRI). The liver segment was complete resected via laparoscopic hepatectomy. The tumor turned out to be inflammatory FDCS. The tumor specimen was margin free. No recurrence was detected at a 12-month follow-upClinical Discussion: Inflammatory FDCS often found in liver and spleen. Taiwan was EBV high-prevalence country, but the occurrence seemed not related. Current management as FDCS, with radiation therapy and chemotherapy if total resection was not feasible.Conclusion: Inflammatory FDCS is a rare malignant tumor in abdomen. Complete resection represents favorable outcome.