bluuebunny/crossref_metadata_2025_split huggingface dataset:
Listeria monocytogenes utilizes the ClpP1/2 proteolytic machinery for fine-tuned substrate degradation under heat stress
Authors: Balogh Dóra , Eckel Konstantin , Fetzer Christian , Sieber Stephan A. 
Published: May 2021

AbstractListeria monocytogenes exhibits two ClpP isoforms (ClpP1/ClpP2) which assemble into a heterooligomeric complex with enhanced proteolytic activity. Herein, we demonstrate that the formation of this complex depends on temperature and reaches a maximum ratio of about 1:1 at heat shock conditions, while almost no complex formation occurred below 4°C. In order to decipher the role of the two isoforms at elevated temperatures, we constructed L. monocytogenes ClpP1, ClpP2 and ClpP1/2 knockout strains and analyzed their protein regulation in comparison to the wild type (WT) strain via whole proteome mass-spectrometry (MS) at 37 °C and 42 °C. While ΔclpP1 strain only altered the expression of very few proteins, ΔclpP2 and ΔclpP1/2 strains revealed the dysregulation of many proteins at both temperatures. These effects were corroborated by crosslinking co-immunoprecipitation MS analysis. Thus, while ClpP1 serves as a mere enhancer of protein degradation in the heterocomplex, ClpP2 is essential for ClpX binding and thus functions as a gatekeeper for substrate entry. Applying an integrated proteomic approach combining whole proteome and co-immunoprecipitation datasets, several putative ClpP2 substrates were identified in the context of different temperatures and discussed with regards to their function in cellular pathways such as the SOS response.
Single-cell transcriptome profiling reveals multicellular ecosystem of nucleus pulposus during degeneration progression
Authors: Tu Ji , Li Wentian , Yang Sidong , Yang Pengyi , Yan Qi , Wang Shenyu , Lai Kaitao , Bai Xupeng , Wu Cenhao , Ding Wenyuan , Cooper-White Justin , Diwan Ashish , Yang Cao , Yang Huilin , Zou Jun 
Published: May 2021

AbstractDegeneration of the nucleus pulposus (NP) is a major contributor to intervertebral disc degeneration (IVDD) and low back pain. However, the underlying molecular complexity and cellular heterogeneity remain poorly understood. Here, we first reported a comprehensive single-cell resolution transcriptional landscape of human NP. Six novel human nucleus pulposus cell (NPCs) populations were identified by distinct molecular signatures. The potential functional differences among NPC subpopulations were analyzed at the single-cell level. Predictive genes, transcriptional factors, and signal pathways with respect to degeneration grades were analyzed. We reported that fibroNPCs, one of our identified subpopulations, might be a population for NP regeneration. CD90+NPCs were observed to be progenitor cells in degenerative NP tissues. NP-infiltrating immune cells comprise a previously unrecognized diversity of cell types, including granulocytic myeloid-derived suppressor cells (G-MDSCs). We uncovered CD11b, OLR1, and CD24 as surface markers of NP-derived G-MDSCs. The G-MDSCs were also found to be enriched in mildly degenerated (grade I and II) NP tissues compared to severely degenerated (grade III and IV) NP tissues. Their immunosuppressive function and protective effects for NPCs were revealed. Collectively, this study revealed the NPC type complexity and phenotypic characteristics in NP, providing new insights and clues for IVDD treatment.
Transcriptional Reprogramming of Skeletal Muscle Stem Cells by the Niche Environment
Authors: Lazure Felicia , Farouni Rick , Sahinyan Korin , Blackburn Darren M. , Hernández-Corchado Aldo , Perron Gabrielle , Ragoussis Jiannis , Crist Colin , Perkins Theodore J. , Jahani-Asl Arezu , Najafabadi Hamed S. , Soleimani Vahab D. 
Published: May 2021

AbstractAdult stem cells are indispensable for tissue regeneration. Tissue-specific stem cells reside in a specialized location called their niche, where they are in constant cross talk with neighboring niche cells and circulatory signals from their environment. Aging has a detrimental effect on the number and the regenerative function of various stem cells. However, whether the loss of stem cell function is a cause or consequence of their aging niche is unclear. Using skeletal muscle stem cells (MuSCs) as a model, we decouple cell-intrinsic from niche-mediated extrinsic effects of aging on their transcriptome. By combiningin vivoMuSC heterochronic transplantation models and computational methods, we show that on a genome-wide scale, age-related altered genes fall into two distinct categories regarding their response to the niche environment. Genes that are inelastic in their response to the niche exhibit altered chromatin accessibility and are associated with differentially methylated regions (DMRs) between young and aged cells. On the other hand, genes that are restorable by niche exposure exhibit altered transcriptome but show no change in chromatin accessibility or DMRs. Taken together, our data suggest that the niche environment plays a decisive role in controlling the transcriptional activity of MuSCs, and exposure to a young niche can reverse approximately half of all age-associated changes that are not epigenetically encoded. The muscle niche therefore serves as an important therapeutic venue to mitigate the negative consequence of aging on tissue regeneration.
Phenotypic plasticity triggers rapid morphological convergence
Authors: Gómez José M. , González-Megías Adela , Narbona Eduardo , Navarro Luis , Perfectti Francisco , Armas Cristina 
Published: May 2021

AbstractPhenotypic convergence, the independent evolution of similar traits, is ubiquitous in nature, happening at all levels of biological organizations and in most kinds of living beings. Uncovering its mechanisms remains a fundamental goal in biology. Evolutionary theory considers that convergence emerges through independent genetic changes selected over long periods of time. We show in this study that convergence can also arise through phenotypic plasticity. We illustrate this idea by investigating how plasticity drivesMoricandia arvensis, a mustard species displaying within-individual polyphenism in flowers, across the morphological space of the entire Brassicaceae family. By compiling the multidimensional floral phenotype, the phylogenetic relationships, and the pollination niche of over 3000 Brassicaceae species, we demonstrated thatMoricandia arvensisexhibits a plastic-mediated within-individual floral disparity greater than that found not only between species but also between higher taxonomical levels such as genera and tribes. As a consequence of this divergence,M. arvensismoves outside the morphospace region occupied by its ancestors and close relatives, crosses into a new region where it encounters a different pollination niche and converges phenotypically with distant Brassicaceae lineages. Our study suggests that, by inducing phenotypes that explore simultaneously different regions of the morphological space, plasticity triggers rapid phenotypic convergence.
The extracellular ATP receptor P2RX7 imprints a pro-memory transcriptional signature in effector CD8+T cells
Authors: Vardam-Kaur Trupti , van Dijk Sarah , Peng Changwei , Wanhainen Kelsey , Jameson Stephen C. , da Silva Henrique Borges 
Published: May 2021

AbstractDevelopment of central memory (Tcm) and resident memory (Trm) CD8+T cells, which respectively promote immunity in the circulation and in barrier tissues, is not completely understood. Tcm and Trm cells may arise from common precursors, however their fate-inducing signals are elusive. We found that virus-specific effector CD8+T cells display heterogeneous expression of the extracellular ATP sensor P2RX7. P2RX7-high expression is confined, at peak effector phase, to CD62L+memory precursors which preferentially form Tcm cells. Among early effector CD8+T cells, asymmetrical P2RX7 distribution correlated with distinct transcriptional signatures, with P2RX7-high cells enriched for memory and tissue-residency sets. P2RX7-high early effectors preferentially form both Tcm and Trm cells. Defective Tcm and Trm formation in P2RX7 deficiency is significantly reverted when the transcriptional repressor Zeb2 is ablated. Our study indicates that unequal P2RX7 upregulation in effector CD8+T cells is a foundational element of the early Tcm/Trm fate.
Modeling investment intention in online P2P lending: an elaboration likelihood perspective
Authors: Lin Chieh-Peng , Huang Hao-Yu 
Published: May 2021

PurposeThis work proposes a research model that explains investment intention in online peer-to-peer (P2P) lending based on the persuasion theory of elaboration likelihood model (ELM). In the proposed model, investment intention indirectly relates to source credibility and argument quality through the mediation of trust. At the same time, the study hypothetically moderates the relationships between source credibility and trust and between argument quality and trust by financial self-efficacy and risk preference.Design/methodology/approachThis research presents an experiment to empirically validate its theoretical rationales. The hypotheses herein were tested using data from working professionals at a large science park in Taiwan. A total of 500 participants took part in the experiment in which the scenario of a pseudo-online P2P lending intermediary was first presented for their perusal, and then questionnaires based on the scenario were provided for the participants to fill out.FindingsTrust cannot be improved over night without making great efforts on source credibility and argument quality in the long run. Online marketers should study market segmentations to decide what appropriate elements and promises should be provided in advertisements in order to improve their source credibility. Moreover, how online intermediaries formulate convincing messages and Polish their delivery communication skills should be improved so as to increase argument quality.Originality/valueFirst, the theoretical conceptualization of source credibility and argument quality built upon the ELM not only broadens the boundary of virtual communities beyond the literature that considers source credibility and argument quality as important determinants, but also shows the practical status quo of trust as a critical mediator. Second, this research incorporates financial self-efficacy (based on social cognitive theory) and risk preference (based on economic theory) as important moderators in the development of trust. For that reason, customer education initiatives that influence financial self-efficacy and risk preference are discussed in greater detail.
Wrong, but not failed? A study of unexpected events and project performance in 21 engineering projects
Authors: Wied Morten , Oehmen Josef , Welo Torgeir , Pikas Ergo 
Published: May 2021

PurposeMost complex engineering projects encounter unexpected events through their life cycle. These are traditionally attributed to inaccurate foresight and poor planning. Outlining a nonanticipatory alternate, the authors seek to explain the ability to rebound from unexpected events, without foresight, using resilient systems theory. This paper seeks to outline the theoretical underpinnings of project resilience and to identify criteria for planning and selecting projects for greater resilience.Design/methodology/approachInvestigating project resilience, this paper studies the relationship between unexpected events and project performance in 21 projects. The authors perform a systematic review of project ex post evaluations 3–12 years after project completion.FindingsFirst, the authors find that all projects encountered unexpected events, even when discounting planning error. Second, the authors show that, as a consequence, projects underperformed, not necessarily relative to formal criteria, but in terms of subjective opportunity cost, that is, relative to competing alternates – known or imagined – foregone by their implementation. Finally, the authors identify four types of resilient projects – superior, equivalent, compensatory and convertible projects – as opportunities for building project resilience.Practical implicationsThe properties of resilient projects provide opportunities for building resilience in complex projects.Originality/valueDeparting from traditional efforts to “de risk” plans and “de-bias” planners, this paper focuses on the properties of projects themselves, as an alternate to improved foresight and up-front planning.
Analysis of Islamic moral principles for sustainable economic development in developing society
Authors: Kalkavan Hakan , Dinçer Hasan , Yüksel Serhat 
Published: May 2021

Purpose
Economic development is one of the primary goals of all countries. In this context, countries aim to determine effective and correct policies to achieve this goal. This situation is especially important for developing countries. These countries aim to grow their economies to reach the level of developed countries. This study aims to identify significant Islamic moral principles for sustainable economic growth of emerging seven (E7) countries.

Design/methodology/approach
In this framework, eight different criteria are defined to improve the welfare in the society for these countries. Additionally, fuzzy decision-making trial and evaluation laboratory (DEMATEL) approach is taken into consideration in the analysis process.

Findings
It is identified that Islamic moral values in economic activities, fair income distribution and taxation and prohibition of interest and securing business partnership are the most significant criteria. On the other side, it is found that preventing poverty and avoiding wastage are the influenced factors.

Practical implications
The findings show that righteousness in commercial activities decreases uncertainty in the market so that trade volume can be increased which contributes sustainable economic improvement. For this purpose, public awareness about the issue should be improved in the media and necessary trainings should be given to the people in their educations related to the business ethics. Furthermore, legal system in these countries should be improved to have fair income distribution and taxation. Additionally, social responsibility activities should be encouraged, so people with good income will help poor people. Moreover, business partnership system should be improved in which companies can obtain capital from the public and make necessary payments to these people according to the profit amount. This issue positively affects sustainability in economic improvement and welfare society for developing countries.

Originality/value
There are limited studies on sustainable economic development based on Islamic moral-based factors.
The perceptions of SME retailers towards the usage of social media marketing amid COVID-19 crisis
Authors: Salam Muhammad Talha , Imtiaz Hamza , Burhan Muhammad 
Published: May 2021

Purpose
During the COVID-19 crisis, diversified attitudes and behaviors of structural equation modeling (SME) retailers were observed in using social media marketing that could have helped mitigate the adverse effects of this crisis on businesses. This paper aims to present a thorough investigation of these perceptions and limited acceptance of social media marketing among SME retailers in a developing country during a crisis.

Design/methodology/approach
The investigation was designed using a mixed-method design. A qualitative investigation, as the first part, explored SME retailers’ perceptions of the use of social media marketing when they were faced with mandatory lockdown that stifled their business activity. The insights from qualitative study and literature helped devise the second part of the study, a quantitative study using the technology acceptance model (TAM). Analysis of responses from a sample of SME retailers (n = 149) was done using SEM in this study.

Findings
In the qualitative study, SME retailers were found to have a varying outlook toward social media marketing. Some ventured into social media marketing while others were impeded by their limited understanding. The second (quantitative) study showed the general applicability of TAM such that perceived ease of use through perceived usefulness influenced SME retailers’ attitudes toward the usage of social media marketing during the COVID-19 crisis. An important finding in both studies was that business owners’ education level influenced their perceptions of social media marketing.

Research limitations/implications
The investigation, albeit a comprehensive one, was conducted in a particular market and for SME retailers. This opens avenues for conducting similar studies in other segments of entrepreneurs to generate insights based on comparative analysis across segments and scenarios.

Originality/value
Limited or no marketing in the physical marketplace amid lockdown meant almost an existential crisis for entrepreneurs, especially SME retailers, in developing countries during the COVID-19 crisis. While technology acceptance by SME retailers has been discussed in the literature, there are limited discourses on technology acceptance among entrepreneurs and SME retailers during a crisis. These findings from the COVID-19 crisis explicate the possibilities and limitations of technology usage as a means to mitigate challenges faced by entrepreneurs during a crisis.
Likely winners and losers in upcoming COVID-19 economic crisis – lessons learned from the GFC
Authors: Čadil Jan , Beránek Marek , Kovář Vladimír 
Published: May 2021

Purpose
The COVID-19 pandemic and subsequent efforts to contain it have started economic downturn that may even surpass the global financial crisis (GFC). The purpose of this study is to uncover the features of enterprises’ resilience during and after an external shock such as the GFC might be helpful in predicting the shock impact on enterprises and setting proper policy measures for the upcoming COVID-19 crisis.

Design/methodology/approach
The authors analysed the impact of the GFC on the entrepreneurial population in/of the Czech Republic using marginal effects method on a large random sample of 4,478 enterprises. In this analysis, the authors defined two groups of enterprises – “winners” and “losers” – based on the company’s dissolution and performance indicators.

Findings
The GFC struck the enterprise population asymmetrically in terms of the enterprises’ characteristics but also in terms of time. Micro and small size companies are the most vulnerable to external shocks such as the GFC. Technological level plays an important role in the recovery phase, especially in the case of manufacturing micro enterprises.

Research limitations/implications
Although there are differences between the GFC and the COVID-19 crisis, the GFC was the only comparable shock in modern history in its global nature, depth and unpredicted occurrence. It can be expected that the impact on enterprises can be partly similar.

Practical implications
Government support of micro size companies should be the priority in the upcoming COVID-19 crisis. Supporting the innovation and technology progress might accelerate the recovery phase after the crisis especially in micro companies as well.

Originality/value
This paper presents interesting insights into the impact of external shocks such as the GFC or COVID-19 on enterprises. It uncovers typical features of “winners” or “losers” of such shocks.
A preliminary study for selecting the appropriate AI-based forecasting model for hospital assets demand under disasters
Authors: Jebbor Sara , Raddouane Chiheb , El Afia Abdellatif 
Published: May 2021

PurposeHospitals recently search for more accurate forecasting systems, given the unpredictable demand and the increasing occurrence of disruptive incidents (mass casualty incidents, pandemics and natural disasters). Besides, the incorporation of automatic inventory and replenishment systems – that hospitals are undertaking – requires developed and accurate forecasting systems. Researchers propose different artificial intelligence (AI)-based forecasting models to predict hospital assets consumption (AC) for everyday activity case and prove that AI-based models generally outperform many forecasting models in this framework. The purpose of this paper is to identify the appropriate AI-based forecasting model(s) for predicting hospital AC under disruptive incidents to improve hospitals' response to disasters/pandemics situations.Design/methodology/approachThe authors select the appropriate AI-based forecasting models according to the deduced criteria from hospitals' framework analysis under disruptive incidents. Artificial neural network (ANN), recurrent neural network (RNN), adaptive neuro-fuzzy inference system (ANFIS) and learning-FIS (FIS with learning algorithms) are generally compliant with the criteria among many AI-based forecasting methods. Therefore, the authors evaluate their accuracy to predict a university hospital AC under a burn mass casualty incident.FindingsThe ANFIS model is the most compliant with the extracted criteria (autonomous learning capability, fast response, real-time control and interpretability) and provides the best accuracy (the average accuracy is 98.46%) comparing to the other models.Originality/valueThis work contributes to developing accurate forecasting systems for hospitals under disruptive incidents to improve their response to disasters/pandemics situations.
Financial health of Syariah and non-Syariah banks: a comparative analysis
Authors: Heniwati Elok , Yantiana Nella , Desyana Gita 
Published: May 2021

Purpose
This paper aims to investigate whether Syariah banks are more financially stable than non-Syariah banks and check the differential impact of explanatory variables in financial health and efficiency in the context of Indonesia.

Design/methodology/approach
By using unbalanced panel data from Bankfocus over the period 2011–2018, regression analysis is performed with two response variables representing financial health, ZSCORE for return on average assets, liquid asset to deposit and short-term funding ratio. A number of control variables are used as tools to confirm the hypotheses. To check the robustness of the findings, a model with different specifications has been used.

Findings
The results indicate that while Syariah banks present higher insolvency risk (less health) for long-term activity, the opposite is true for short-term activity. Other findings show that Syariah and non-Syariah banks contribute differently to the national system of financial stability owing to varying influential factors on the bank’s health.

Originality/value
This paper presents a comparative analysis between the financial stability of Syariah banks and that of non-Syariah banks in Indonesia by building an empirical framework that allows the author to examine the differential effects of each underlying feature on financial stability in Syariah and non-Syariah banks.