bluuebunny/crossref_metadata_2025_split huggingface dataset:
Development of a validated quantitative polymerase chain reaction assay and fungal culture for the diagnosis of Macrorhabdus ornithogaster in budgerigars (Melopsittacus undulatus)
Authors: Lang Danielle M. , Adamovicz Laura A. , Hung Chien-Che , Delk Katie W. , Langan Jennifer N. , Chinnadurai Sathya K. , Allender Matthew C. 
Published: February 2025

Abstract

Objective
To develop and validate a quantitative PCR (qPCR) assay for detecting Macrorhabdus ornithogaster (MO) and reproducibly culture MO from infected budgerigars (Melopsittacus undulatus).

Methods
A TaqMan qPCR assay targeting a 94-bp segment of MO 18S rRNA was evaluated for limit of detection, dynamic range, intra-assay variability, interassay variability, and efficiency. Proventricular-ventricular samples and feces from deceased budgerigars positive for MO on cytology were plated with Basal Medium Eagle or chicken serum media, 20% fetal bovine serum, 5% sucrose, 100 U/mL penicillin, 100 µg/mL streptomycin, and 25 µg/mL chloramphenicol at pH 3 to 4 and 42 °C under microaerophilic conditions.

Results
The qPCR was successfully developed and performed with high efficiency (slope = −3.355; R2 = 0.999; efficiency = 98.622) and low intra- and interassay variability (coefficient of variation < 2.63% at all dilutions). The dynamic range was 107 to 101 copies/reaction with a limit of detection of 10 target copies/reaction. Macrorhabdus ornithogaster was successfully cultured from 4 different infected budgerigars using this culture protocol; however, cultures did not maintain long enough for antifungal susceptibility testing.

Conclusions
We developed and analytically validated a TaqMan qPCR assay for MO detection. Macrorhabdus ornithogaster culture is possible, but further research is needed for culture maintenance and susceptibility testing.

Clinical Relevance
This analytically validated qPCR MO assay will be a useful diagnostic tool for the detection and quantification of MO in infected budgerigar feces. Reliable culturing of MO will provide the basis for antifungal drug susceptibility testing to improve treatment methods for MO in birds.
Can interprofessional education that embeds One Health be an effective platform for climate change education in veterinary and medical curricula?
Authors: Roopnarine Rohini , Webb Tracy L. , Riles Anuja , Tucker Claire , Watson Brittany , Chun Ruthanne 
Published: February 2025

Abstract
Climate change has been recognized as a significant public health threat, and education is needed to allow health professionals to better care for their patients affected by climate-related conditions. Medical students concerned about their lack of preparedness to respond to its effects on patient health, created the Planetary Health Report Card, calling for the inclusion of planetary health education into curricula. Veterinary students similarly express their lack of readiness in this area for practice. In alignment with the United Nations, the American Medical Association (AMA) and AVMA, promulgate educators’ development of climate education to prepare graduates for future practice. Implementing interprofessional education is a critical paradigm for closing the gaps on climate education in these curricula. Incorporating One Health related competencies into an interprofessional education curriculum would prepare future graduates to effectively respond to this threat.
Monitoring of Wastewater Parameters in Treatment Plants: Case Study – WWTP of Iasi City
Authors: Prăjanu Costel-Cătălin , Stătescu Florian , Toma Daniel , Toma Cristina-Mihaela Vîrlan 
Published: February 2025

Abstract
This article briefly presents how to monitor a technological process in a municipal WWTP, in order to better understand the treatment processes. Monitoring the parameters of wastewater in a treatment plant, involves monitoring the quality of wastewater through measuring and control equipment, sampling wastewater as accurately and as many points as possible, the ideal case being after each technological object or treatment step and analysis of wastewater samples as accurately as possible, also the ideal case being compliance with well-defined methodologies. Following the monitoring of wastewater parameters, data and study results are obtained that can be used to evaluate the technological process, make graphs and tables or to optimize technological processes. The research methodology consists in the presentation of the way of monitoring the wastewater parameters with the measuring and control equipment, the presentation of the sampling method and the analysis method, after which the obtained data will be presented graphically and tabularly.
Application of Geostatistical Methods in the Study of Algae in Water Reservoirs: A Review
Authors: Zawadzki Jarosław , Fabijańczyk Piotr 
Published: February 2025

Abstract
The growing pollution of aquatic environments, primarily of anthropogenic origin, combined with global climate change, has led to significant increases in eutrophication. This process often results in harmful algal blooms (HABs) of phytoplankton and algae in various water bodies, including inland lakes, marshes, rivers, seas, and oceans. These blooms pose a serious threat not only to aquatic ecosystems but also to human health. Understanding phytoplankton and algal blooms is inherently complex, as these phenomena manifest on multiple spatial and temporal scales. Comprehensive studies of phytoplankton and algae require the collaboration of scientists from diverse scientific disciplines, including biology, ecology, and environmental science. One of the critical tools in this multidisciplinary approach is geostatistics, an advanced and continuously evolving branch of statistics that specialises in analysing spatial and temporal phenomena. Geostatistics is particularly well-suited for the study of phytoplankton and algal blooms due to its ability to handle data that varies across different scales and locations. This review presents and discusses selected studies that employ geostatistical methods to investigate plankton and algae in various water bodies. It highlights the most significant scientific works that, in the authors’ opinion, represent milestones in the application of these studies. Furthermore, various geostatistical methods are explored, ranging from variography to spatiotemporal modelling, providing insights into spatial and temporal patterns and their variability of phytoplankton and algal blooms in aquatic ecosystems.
Optimising Low Carbon Inventory Decisions and Coordination for Used Clothing Under Carbon Tax and Carbon Labelling System
Authors: Gao Honghu , Tang Jialin Karen , Gong Daqing , Zhao Xiaoxia , Yan Xiaojie 
Published: February 2025

Abstract
This paper investigates the trade-off between carbon emissions and profitability in used clothing inventory management under carbon labelling and taxation policies. The study analyses various decision models within a two-tier supply chain comprising a single supplier and retailer, deriving optimal solutions for inventory carbon emission levels, sales prices, wholesale prices, and profit maximisation for each supply chain participant. Our findings reveal several key patterns: For environmentally conscious suppliers, both centralised and decentralised decision-making models show that increasing carbon tax rates lead to decreased inventory carbon emission levels of used clothing. When carbon tax remains constant, heightened consumer awareness results in reduced inventory carbon emission levels. For pollution-intensive suppliers under decentralised decision-making, the inventory carbon emission levels follow a U-shaped trend. Similarly, when carbon tax is fixed, increasing consumer awareness of carbon labels also produces a U-shaped trend in suppliers’ inventory carbon emission levels. Under centralised decision-making, higher carbon tax rates consistently lead to decreased inventory carbon emission levels. Additionally, stronger consumer preferences for low-carbon labels result in reduced used clothing inventory carbon emission levels. When the baseline of low carbon levels is identical, carbon tax and carbon regulation intensity demonstrate similar effects on inventory carbon emission levels, demand, supply chain profits, and coordination mechanisms, while exhibiting opposite effects on sales prices. The study achieves supply chain coordination through an optimal wholesale price contract based on Nash bargaining, with numerical analysis validating the proposed model.
Synthesis of Sustainable Superabsorbent Biopolymer: Modified Rice Straw Cellulose with Initiator and Crosslink Agent
Authors: Susmanto Prahady , Putri Anggi Junia , Parwati Ajeng 
Published: February 2025

Abstract
Superabsorbent biopolymers are polymer-based materials that have high absorption capabilities made from synthetic monomers modified with natural monomers such as cellulose. The addition of cellulose as a natural monomer is to produce a product with superabsorbent properties biodegradable so as not to have a negative impact to the environment. The aim of this research was to determine the potential of rice straw cellulose to be used as a natural monomer in the manufacture of superabsorbent biopolymers. The preparation of this superabsorbent biopolymer was carried out by varying the concentration of the initiator and crosslink agent. Initiator variation potassium persulfate (KPS) namely 0.2 %; 0.4 %; 0.6 %; and 0.8 %; meanwhile, variation crosslink agent in the form of citric acid was 0 g, 2 g, 4 g, 6 g, and 8 g with the addition of 20 g acrylamide. Functional group analysis was carried out by testing Fourier Transform Infrared (FTIR) indicating the presence of -OH, C-H, and C=C functional groups in rice straw cellulose that shows the potential to be the material for producing superabsorbent polymer. The Scanning Electron Microscope (SEM) analysis showed that the morphology of superabsorbent, the surface of the superabsorbent biopolymer looks denser, this indicates that there has been a bond formation between the monomer and crosslink agent. The results showed that the superabsorbent biopolymer produced with variations in initiator concentrations of 0.6 % and crosslink agent 6 g has a water absorption capacity and swelling in solution with buffer acetate the best were 1304.3 % and 10.26 w/w respectively.
Parameter Tuning of A Binary Pareto Whale Optimization Algorithm Using Taguchi Grey Relational Analysis
Authors: Haleel Aseel Jameel , Dawood Lamyaa Mohammed 
Published: February 2025

Abstract
Parameter values of any metaheuristic algorithm affect the performance of the algorithm search. However, using statistics to estimate the proper values for the algorithm’s parameters will be feasible to make optimization algorithm more robust and effective. The aim of this paper is to investigate the optimal control parameter values for the Binary Pareto Whale Optimization Algorithm BPWOA, which is used for solving maintenance scheduling problem at a power plant. Three algorithm control parameters involving population size, iteration number, and archive size were fine-tuned using Taguchi-Grey Relational Analysis GRA to achieve an optimal maintenance schedule with maximum power supply, minimum fuel expense, and minimum Carbone Dioxide CO2 emissions. The algorithm runs carried out based on Taguchi experiment design using L25 orthogonal array. The Grey Relational Grade GRG metric is utilized to evaluate the BPWOA performance. The results show that the Taguchi-Grey relational analysis approach is a dependable and efficient way to generate new optimal values for the BPWOA control parameters, allowing for multi-objective power plant maintenance scheduling with fewer runs in less time and a 20% improvement in GRG of objectives.
PENINGKATAN SKILL NARAPIDANA LEMBAGA PEMASYARAKATAN LOWOKWARU MALANG MELALUI KEGIATAN BUDIDAYA IKAN LELE DENGAN TEKNOLOGI RESRKULASI AKUAKULTUR SISTEM (RAS) DAN BUDIDAYA PAKAN ALAMI
Authors: Ramadhani Putri , Irawan Phobie Yolasica , Zahroh Fathimatuz , Wahyudi Moh. Ilham , Ghifari Maulana Zaky , Handajani Hany 
Published: February 2025

Kegiatan pengabdian masyarakat peningkatan soft skill dan hard skill narapidana lapas kelas 1 Malang dengan mengupayakan kemandirian pangan melalui budidaya ikan lele dengan teknologi Resirkulasi Akuakultur Sistem dan budidaya pakan alami. Kegiatan dilatar belakangi beberapa permasalahan yaitu sulitnya mencari pekerjaan bagi narapidana saat keluar dari Lapas dan petugas tidak bisa mendampingi narapidana, kurangnya kegiatan mengisi waktu luang dan minimnya ketersediaan protein hewani. Metode pelaksanaan program meliputi penyuluhan, pelatihan dan pendampingan. Tujuannya untuk memberikan skill pada narapidana dan petugas Lapas tentang budidaya ikan teknologi RAS dan pakan alami. Memanfaatkan waktu luang sehingga menanbah waktu produktif, serta memenuhi kebutuhan protein hewani narapidana. Metode program dilakukan secara offline dengan diawali kegiatan penyuluhan dan pelatihan, pembuatan rak dan penebaran Tubifex sp., budidaya Daphnia sp. Pembuatan teknologi RAS, penebaran ikan lele ke kolam terpal, monitoring, pemanenan pakan alami, dan pemanenan ikan lele sistem teknologi RAS. Kegiatan ini secara keseluruhan mendapatkan hasil yang baik dan memuaskan. Tim PKM PM secara rutin melakukan pendampingan dalam pelaksanaan pengabdian. Hal ini diketahui dari pemahaman narapidana dalam menerapkan program budidaya ikan dengan teknologi resirkulasi akuakultur sistem (RAS) dan budidaya pakan alami di Lapas Kelas 1 Malang, yakni pemeliharaan ikan dan pakan alami Tubifex sp. dan Daphnia sp. hingga proses pemanenan dan terpenuhinya protein hewani narapidana. Serta terwujudnya penambahan waktu produktif bagi narapidana. Sehingga harapan tiga tahun kedepan para narapidana dapat melanjutkan proses budidaya ikan dan budidaya pakan alami yang berkelanjutan. Program dapat terwujud melalui 10 kegiatan yang sudah dilakukan berupa budidaya ikan teknologi resirkulasi akuakultur sistem dan budidaya pakan alami.
PENERAPAN DIGITAL MARKETING DAN PEMBUATAN NIB SEBAGAI STRATEGI PEMASARAN UMKM
Authors: Kader Mukhtar Abdul , Toto Toto , Puspita Endah , Cahyani Ika , Pauji Irfan Hilmi 
Published: February 2025

Desa Kertayasa merupakan salah Desa Swadaya dimana letaknya cukup jauh dari Kab. Ciamis dan Sulitnya Akses Internet, dengan kegiatan Kampus Merdeka pada program Merdeka Belajar Kampus Merdeka dimana salah satu programnya adalah Kuliah Kerja Nyata Tematik yang dilaksanakan selama satu semester. Pemilihan Desa kertayasa oleh karena peringkat desa yang di peroleh perlu di tingkatkan menjadi Desa mandiri, letaknya yang cukup jauh dari Kota Ciamis sehingga akses internet menjadi sangat sulit, diharapkan Desa kertayasa dimasa datang mampu meningkatkan levelnya menjadi desa swadaya: masyarakatnya mampu menggunakan internet untuk meningkatkan penjualannya. Kegiatan KKNT ini memfokuskan peningkatan kemampuan UMKM dengan menggunakan berbagai media online. Metode yang digunakan adalah melakukan pendampingan langsung bersama warga terutama para remaja yang sudah memiliki kompetensi literasi digital marketing cukup baik kepada usaha kecil mikro mulai dari pengurusan perizinan, kemasan produk, pembuatan marketplace dan metode pemasaran dengan konsep digital marketting. Usaha Mikro kecil dan Menengah yang ada di desa kertayasa Beberapa Usaha mikro telah memiliki Nomor Induk Berusaha (NIB) sebagian besar belum memiliki sementara pemanfaatan digital marketing masih sangat kurang sehingga dilaksanakanlah pelatiahan sekaligus pendampingan implementasi digital marketing. sebagai identitas dan meningkatkan kepercayaan konsumen, pembuatan media sosial antara lain mulai dari Instagram Bisnis, Fans Page, Facebook Profesional, Youtube ads. Bagi Usaha Mikro yang memiliki banyak jenis produk diarahkan ke marketplace seperti shopee dan tokopedia. Selanjutnya berkolaborasi dengan masyarakat terutama pemuda karang taruna dalam penyebaran informasi produk melalui konsep digital marketing. Kerjasama fakultas ekonomi dengan desa kertayasa melalui pembuatan NIB Usaha Mikro, Pendampingan Digital Marketing mampu meningkatan luas pasar, kompetensi warga.
INOVASI TEKNOLOGI PIROLISIS UNTUK PENINGKATAN KESEJAHTERAAN PETANI KOPI HUTAN DI DESA KRETEK BONDOWOSO
Authors: Putri Resti Pranata , Yudiastuti Silvia Oktavia Nur , Yossi Wibisono , Hernowo Pandit , Handayani Wiwik , Arumsari Alif Gita , Halim Abdul 
Published: February 2025

Kelompok Tani Hutan (KTH) Sejahtera didirikan untuk meningkatkan kesejahteraan masyarakat di Desa Kretek, Kecamatan Taman Krocok, Kabupaten Bondowoso. KTH ini mengelola lahan kopi robusta dengan produksi tahunan 500-800 kg. Namun, selama 11 tahun, lahan ini tidak dipupuk, menyebabkan penurunan produktivitas tanah dan kualitas biji kopi. Untuk mengatasi hal tersebut, teknologi pirolisis diperkenalkan guna memanfaatkan limbah kulit kopi. Teknologi ini menghasilkan biochar yang dapat meningkatkan kesuburan tanah, mengikat zat pencemar, serta mengurangi emisi gas rumah kaca. Teknologi ini juga menghasilkan asap cair yang dapat dimanfaatkan sebagai antimikroba dan bahan pengawet makanan. Tujuan kegiatan ini adalah membantu masyarakat memanfaatkan limbah kulit kopi menjadi produk unggulan. Metode yang digunakan adalah participatory rural appraisal, yang meliputi introduksi inovasi pemanfaatan limbah kulit kopi menjadi biochar dan asap cair, perancangan dan pembuatan alat, serta praktik penggunaannya dengan melibatkan anggota KTH Sejahtera. Hasil kegiatan pengabdian ini menunjukkan terjadinya peningkatan pengetahuan serta ketrampilan kelompok tani hutan dalam pembuatan biochar dan asap cair menggunakan teknologi pirolisis dari semula rata-rata 30% menjadi 90%. Secara keseluruhan, program pengabdian ini dapat meningkatkan semangat dalam pemanfaatan limbah kulit kopi dan meningkatkan kekompakan KTH Sejahtera.
SOSIALISASI DAN PENDAMPINGAN PEMBUATAN ENKAPSULASI PUPUK ORGANIK GRANULAR DARI LIMBAH KOTORAN SAPI MENGGUNAKAN BAKTERI RHIZOBIUM SPP. BAGI MASYARAKAT TANI DESA MAYANGAN, KECAMATAN GUMUKMAS, KABUPATEN JEMBER
Authors: Soeparjono Sigit , Khozin Mohammad Nur , Putri Widya Kristiyanti , Siswoyo Tri Agus , Slameto Slameto , Niagra Risnanda Deva Martha , Ahnaf Yusuf Dary 
Published: February 2025

Peningkatan harga dan terbatasnya ketersediaan pupuk bersubsidi menyebabkan petani mencari alternatif pupuk yang lebih terjangkau dan tersedia untuk mendukung kesinambungan usaha budidaya pertanian. Salah satu pupuk yang sering digunakan masyarakat adalah pupuk organik yang berasal dari limbah kotoran hewan ternak sapi. Desa Mayangan, Kecamatan Gumukmas, Kabupaten Jember memiliki potensi limbah kotoran sapi yang melimpah dari usaha pertenakan yang di lakukan oleh masyarakat desa setempat. Sebagian besar para petani masih menggunakan cara konvensional sehingga membutuhkan waktu relatif lama. Pengabdian desa binaan Universitas Jember di Desa Mayangan ini bertujuan untuk membantu penyediaan pupuk organik bagi petani dan mempercepat waktu proses pembuatan pupuk dari limbah kotoran sapi dengan aplikasi teknologi enkapsulasi pupuk organik granular. Diharapkan kegiatan pengabdian ini mampu mendukung program pertanian berkelanjutan dan ketahanan pangan. Adapun tahapan pengabdian yang direncanakan meliputi sosialisasi kegiatan dan edukasi, pendampingan pembuatan pupuk organik granular berbahan dasar limah kotoran sapi, penerapan teknologi enkapsulasi, dan Focus Group Discussion (FGD). Program strategis sesuai RIP UNEJ yang diacu yaitu pengembangan dan penerapan teknologi unggulan tepat guna untuk mendukung peningkatan kesejahteraan masyarakat melalui program desa mitra unggulan. Hasil kegiatan pengabdian ini dapat disimpulkan bahwa dari 25 responden menunjukkan 100% peserta mengenal pupuk granula dari kompos kotoran sapi dan mengenal bakteri Rhizobium sp., serta 92% mampu membuat enkapsulasi pupuk dari limbah kotoran sapi dengan bakteri Rhizobium sp.
MEMANEN AIR HUJAN; PEMBERDAYAAN MASYARAKAT DALAM PENGELOLAAN AIR UNTUK KEHIDUPAN YANG RESILIEN DI SENGKOL LOMBOK TENGAH
Authors: Azmiyati Uzlifatul , Littaqwa Lalu Auliya Aqraboe , Side Gagassage Nanluih De 
Published: February 2025

Kelompok Perempuan Pemerhati Lingkungan (KPPL) Sejahtera dan Sumber Jaya berlokasi di kabupaten Rejang lebong, Provinsi Bengkulu. Kelompok ini telah dibentuk dalam koperasi dan memiliki beberapa olahan pangan. Bahan Baku yang menjadi fokus dalam kelompok adalah nangka dan alpukat. Tujuan dari kegiatan pengabdian ini meningkatkan pengetahuan dan keterampilan kelompok perempuan pemerhati lingkungan (KPPL) dalam pengembangan olahan nangka menjadi abon berbasis pengawetan dan peningkatan nilai gizi. Metode pengabdian terdiri dari survei, sosialisasi dengan pemaparan materi, praktek secara langsung tentang pembuatan olahan abon nangka ikan, proses pengawetan nangka dengan kombinasi ikan, proses pengolahan dan rancangan evaluasi kegiatan dengan melihat hasil pre-test dan post-test. Tingkat pemahaman peserta terhadap produk nangka apakah perlu diawetkan, beberapa jenis pengawet makanan dan nangka dapat dijadikan abon saat dilakukan pretest sudah tinggi yakni diatas 70% (86.96%, 95.65% dan 91.30%). Kombinasi antara produk nangka dan ikan, serta bahan pertanian dan perikanan dapat digunakan untuk pengawet makanan pada saat pre-test perlu di highligh (disoroti) dengan peresentase peserta yang mengetahui itu masih dibawah 70 % (65.22 dan 26.09). Secara menyeluruh peserta mengalami peningkatan pengetahuan saat sebelum kegiatan (hasil pre-test) dan setelah kegiatan (hasil post-test).