bluuebunny/crossref_metadata_2025_split huggingface dataset:
Validation of Drought Tolerance Gene-linked Microsatellite Markers and Their Efficiency for Diversity Assessment in a Set of Soybean Genotypes
Authors: Mishra Nishi , Tripathi M. K. , Tripathi Niraj , Tiwari Sushma , Gupta Neha , Sharma Akash 
Published: October 2021

Aim: Soybean is well-thought-out to be a major crop owing to its significant involvement as vegetable oil and protein in human diet. However, inopportunely, its production has been melodramatically declined attributable to the commonness of drought related stress.
Study Design: During the present study a total of 53 soybean genotypes were selected. For molecular diversity analysis as well as validation total 12 SSR markers were used. Molecular screening of soybean genotypes was done to determine the efficiency of available markers in genetic diversity analysis as well as their validation on the basis of their association with drought tolerance gene.
Place and Duration of the Study: The present study was conducted at Department of Plant Molecular Biology and Biotechnology, College of Agriculture, Gwalior, Rajmata Vijayaraje Scindia Krishi Vishwa Vidyalaya, Gwalior, M.P., India during the year 2018 - 2019.
Methodology: Template DNA of all 53 selected soybean genotypes extracted for molecular screening. The current investigation has been accomplished to validate the available SSR markers with their efficiency in genetic diversity analysis in a set of soybean genotypes.
Results: Among applied drought tolerance gene-linked 12 SSR molecular markers, the highest genetic diversity (0.6629) was noticed in Satt520 while lowest (0.0370) was in Satt557 with an average of 0. 3746.While, the highest PIC value was 0.5887 prearranged by Satt520 and lowest 0.0363 by Satt557 with the mean worth of 0.3063.
Conclusion: Dendrogram constructed on the basis of banding profile of employed markers was able to discriminate some putative drought tolerant genotypes i.e., JS97-52, JS95-60 from rest of the genotypes. The results of the present examination may donate towards enhancement of soybean genotypes to bread drought tolerant varieties.
Performance Evaluation of DSSAT CERES Wheat Model under Different Thermal and Irrigation Regimes at Prayagraj
Authors: Prasanna Aggile Lakshmi , Rawat Shradhha , Gautam Shweta 
Published: October 2021

The present study was planned to determine the phenology and heat unit requirement of promising wheat varieties under different crop growing environment of Prayagraj, India. Delay in sowing of wheat results into reduction in the grain yield as the crop exposes to high temperature at phenological stages. A field experiment on wheat (variety PBW-502) was conducted at the farm nursery, College of Forestry, SHUATS, Prayagraj during rabi seasons of 2018-19 and 2019-20. The experiment was conducted in factorial randomized block design and replicated thrice with three dates of sowing and five irrigation levels. The yield attributes (grain and biological yield) were significantly influenced by sowing dates and irrigation levels. The significantly highest grain and biological yield were recorded in crop sown on 15th November as compared to other dates of sowing. Among different irrigation levels, grain and biological yield were significantly more in I5 (CRI+ tillering + jointing + anthesis + maturity) treatment over the other irrigation levels. DSSAT CERES-Wheat model suggests good model performance at optimum irrigation at Prayagraj under stress condition.
Antioxidant Property and Oral Glucose Tolerance of Stem Extract of Andrographis paniculata – Nees
Authors: Awoyinka E. O. , Ogundare A. O. , Adebolu T. T. , Isaac Adeloye Adedeji 
Published: October 2021

This research aims to investigate the antioxidant property and oral glucose tolerance of stem extract of Andrographis paniculata using standard methods. The antioxidant activity of the extracts were assessed using total phenol, total flavonoid, ABTS (2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid)), nitric oxide, GSH (gluthatione), super oxide, DPPH [1,1-di-phenyl-2-picryl hydrazyl] and ascorbic acid. The oral glucose tolerance of methanol stem extract of A. paniculata was carried out using wistar albino rats. The Wistar albino rats were divided into six groups consisting of five rats each. Group 1 was given water only, group two to group five were induced with 2g/kg of glucose. After 30 minutes, group 3 and 4 were treated with the doses of 15 mg/kg and 30 mg/kg of the extract while group 5 was treated with glibenclamide respectively. The blood glucose level of each group was monitored for 2hours at 30 minutes interval and compared with the control that was given water only. The result shows that the extracts were able to scavenge free radicals but methanol stem extract of A. paniculata performed better in scavenging free radicals as compared to others. The blood sugar levels of the rats treated with doses of 15 and 30 mg/kg of methanol stem extract of A. paniculata were significantly lower than the group of rats that were fed with only glucose and the group of rats treated with glibenclamide. The glucose-lowering efficiency of the extract was between 60-120 minutes. Findings from this present study revealed that A. paniculata is a potential plant that can scavenge free radicals and also possess hypoglycemic activity.
Fetal Outcomes of Abnormal Placental and Birth Weight Ratio
Authors: Aslam Uzma , Raishem . , Kashif Asma , Dahri Aisha , Khanam Azra , Iqbal Mahwish , Ali Aneela 
Published: October 2021

Objective: To find out the fetal complications of abnormal placental and birth weight ratio.
Materials and methods: This is a cross sectional study conducted from January 2019 to January 2020 at department of Gyn/Obs PMCH Nawabshah. Total 110 patients who met the criteria were included in this study. After history, clinical examination and required radiological and biochemical investigations, participants were delivered and weight of placenta and bay was measured and ratio was recorded. Results were made and conclusion was drawn.
Results: Age difference was also seen in participants. Maternal age ranged from 18-35 years. 65(59%) patients ranged from 18 to 25 years. 30(27%) patients were of age between 26-30 years. 16(14%) aged from 31-35 years. Regarding fetal outcomes, Intra Uterine Growth Retardation (IUGR) was found to be among 30(27.27%), IUD in 3(2.72%), fetal distress in10 (9.09%), low APGAR in 7(6.36%), respiratory distress in 6(5.45%) and Cardio Vascular Diseases in 0% patients.
Conclusion: To sum up, it is concluded that in our study, the common fetal outcome due to abnormal Abnormal Placental And Birth Weight Ratio (PBWR) was intra uterine growth retardation (IUGR) followed by IUD, fetal distress and low APGAR.
Knowledge of Healthcare Professionals Regarding the Primary Management of Anaphylaxis in Madinah, Saudi Arabia
Authors: AlHaddad Nasser S. , Alshaeri Abdul Rahman A. , Faidh Athar A. , Alahmadi Yaser M. , Elbadawy Hossein M. , Aldhafiri Ahmed J. 
Published: October 2021

Aim: To assess the knowledge, attitude and practice of healthcare professionals regarding the anaphylaxis in Medina, Saudi Arabia.
Settings and Design: This was a population-based epidemiological study using a survey composed of 20 questions about the causes, symptoms, and treatment of anaphylaxis.
Methods: This survey was distributed to healthcare professionals who represented different job categories including consultants, specialists, interns, residents, nurses, and medical students.
Statistical Analysis Used: One-way ANOVA is used to compare the knowledge score across different job categories. SPSS V26 software was used for the analysis and a p value of <0.05 was considered significant.
Results: The mean of the correct answers for all participants was 60% and no difference in the mean scores was found in all participant from different job categories. The minimum achieved score was 30% and the maximum score was 100%. The score for the correct answers to each question was varied from 21% to 81%.
Conclusions: The shortage of knowledge among the healthcare professionals regarding anaphylaxis predicts the occurrence of undesirable outcomes for patients. Educating healthcare professionals regarding the management of anaphylaxis can improve the quality of healthcare services.
Work-related factors among people with diabetes and the risk of cardiovascular diseases: A systematic review
Authors: Saif-Ur-Rahman K M , Mamun Razib , Li Yuanying , Matsunaga Masaaki , Ota Atsuhiko , Yatsuya Hiroshi 
Published: October 2021

Abstract

Background
Diabetes is a major risk factor for cardiovascular diseases (CVD). This systematic review aims to explore the work-related factors among people with diabetes in developing CVD.

Methods
Four electronic databases were searched on 1 February 2021 using a comprehensive search strategy without any time restriction. Two independent researchers screened the articles and extracted data. The risk of bias was assessed independently using the risk of bias assessment tool for non-randomized studies (RoBANS). A narrative synthesis was conducted considering the heterogeneity of the included articles.

Results
A total of five articles incorporating 4 409 810 participants from three geographic regions were included that highlights the research gap. As per the included studies, Occupational drivers with diabetes were at a higher risk of CVD in comparison to the nondrivers, workers with diabetes having a long working hour were at a higher risk of CVD mortality, workers with a lower occupational status were at a higher risk of 10-years stroke risk, and occupational physical activity and occupational commuting lowered the risk of CVD deaths.

Conclusions
This systematic review summarized the available evidence on work-related factors influencing the risk of CVD in people with diabetes. The findings should be interpreted cautiously pondering the limited evidence and imprecision. We identified only five articles related to the topic, and there were no studies from Japan. The scarcity of studies on work-related factors on the prognosis of diabetic patients implies the need for more research in this field. We recommend further exploration of the topic designing primary studies.
Dosimetric impact of simulated changes in large bowel content during proton therapy with simultaneous integrated boost for locally advanced pancreatic cancer
Authors: Narita Yuki , Kato Takahiro , Takemasa Kimihiro , Sato Hiroki , Ikeda Tomohiro , Harada Takaomi , Oyama Sho , Murakami Masao 
Published: October 2021

AbstractPurposeTo investigate the dosimetric impact of changes in the large bowel content during proton therapy (PT) with simultaneous integrated boost (SIB) for locally advanced pancreatic cancer (LAPC).Materials and methodsFifteen patients with LAPC were included in this study. The SIB method was performed using five fields according to our standard protocol. A total dose of 67.5 Gy(relative biological effectiveness [RBE]) was prescribed in 25 fractions using the SIB method. A dose of 45 Gy(RBE) was prescribed for the entire planning target volume (PTV) by using four main fields. The remaining 22.5 Gy(RBE) was prescribed to the PTV excluding for the gastrointestinal tract using one subfield. Five simulated doses were obtained by the forward dose calculations with the Hounsfield units (HU) override to the large bowel to 50, 0, −100, −500, and −1000, respectively. The dose‐volume indices in each plan were compared using the 50 HU plan as a reference.ResultsAt D98 of the clinical target volume (CTV) and spinal cord‐D2cc, when the density of the large bowel was close to that of gas, there were significant differences compared to the reference plan (p < 0.05). By contrast, no significant difference was observed in stomach‐D2cc duodenum‐D2cc, small bowel‐D2cc, kidneys‐V18, and liver‐Dmean under any of the conditions. There were no cases in which the dose constraint of organs at risk, specified by our institution, was exceeded.ConclusionDensity change in the large bowel was revealed to significantly affect the doses of the CTV and spinal cord during PT with SIB for LAPC. For beam arrangement, it is important to select a gantry angle that prevents the large bowel from passing as much as possible. If this is unavoidable, it is important to carefully observe the gas image on the beam path during daily image guidance and to provide adaptive re‐planning as needed.
Initial Formation of the Skin Layer of PLGA Microparticles
Authors: Sharifi Farrokh , Otte Andrew , Park Kinam 
Published: October 2021

AbstractPoly(lactide‐co‐glycolide) (PLGA) has been extensively used in making long‐acting injectable formulations. The critical factors affecting the PLGA formulation properties have been adjusted to control the drug release kinetics and obtain desirable properties of PLGA‐based drug delivery systems. The PLGA microparticle formation begins as soon as the drug/PLGA‐dissolved in the organic solvent phase (oil phase) is exposed to the water phase. The initial skin (or shell) formation on the oil droplets occurs very quickly, sometimes in the matter of milliseconds, and studying the process has been difficult. The skin formation on the PLGA emulsion droplet surface that can affect the subsequent hardening steps is examined. PLGA droplets with different compositions are prepared. Using collimated light and a high‐speed camera made it possible to detect the diffusion of acetonitrile from the oil phase into the water phase during the oil droplet formation. Although the skin formation is not visible on the surface of the oil phase droplet with the current setup, the droplet shapes, solid strand formation, and the difference in the spreading time suggest that the initial contact time between the oil and water phases in the range of a few seconds is critical to the properties of the skin.
Bioinspired Artificial Liver System with hiPSC‐Derived Hepatocytes for Acute Liver Failure Treatment
Authors: Wang Jinglin , Ren Haozhen , Liu Yuxiao , Sun Lingyu , Zhang Zhuohao , Zhao Yuanjin , Shi Xiaolei 
Published: October 2021

AbstractBioartificial liver (BAL) system has become a promising alternative to traditional liver transplantation in rescuing acute liver failure (ALF) patients. Herein, inspired by natural microstructure of hepatic lobules, a novel biomimetic bioartificial liver system (BBALS) is developed by integrating human induced pluripotent stem cell‐derived hepatocytes (hiPSC‐Heps) ‐laden microparticles and semipermeable microtubes into a microfluidic platform. As the working units are hepatic lobules‐like semipermeable microtubes surrounding with serum‐free suspension differentiated hiPSC‐Heps microcarriers, the BBALS is endowed with functional cell aggregates and effective circulation system. Thus, the BBALS possesses high cell viability, favorable function regeneration, and effective substances exchange. Based on these features, a 3D liver chip with multiple parallel BBALS units is created for filtering the plasma of ALF rabbits, which validates the research significance and application potential of the proposed BBALS. Moreover, the novel integrated BBALS is applied to treat ALF rabbits and shows great advantages in increasing survival, generating serum proteins, and decreasing inflammation. These properties point to the broad prospects of BBALS in treating related diseases and improving traditional clinical methods.
Mechanistic Probing of Encapsulation and Confined Growth of Lithium Crystals in Carbonaceous Nanotubes
Authors: Wei Ping , Cheng Yong , Yan Xiaolin , Ye Weibin , Lan Xiangna , Wang Lina , Sun Jingjie , Yu Zhiyang , Luo Guangfu , Yang Yong , Rummeli Mark H. , Wang Ming‐Sheng 
Published: October 2021

AbstractEncapsulation of lithium in the confined spaces within individual nanocapsules is intriguing and highly desirable for developing high‐performance Li metal anodes. This work aims for a mechanistic understanding of Li encapsulation and its confined growth kinetics inside 1D enclosed spaces. To achieve this, amorphous carbon nanotubes are employed as a model host using in situ transmission electron microscopy. The carbon shells have dual roles, providing geometric/mechanical constraints and electron/ion transport channels, which profoundly alter the Li growth patterns. Li growth/dissolution takes place via atom addition/removal at the free surfaces through Li+ diffusion along the shells in the electric field direction, resulting in the formation of unusual Li structures, such as poly‐crystalline nanowires and free‐standing 2D ultrathin (1–2 nm) Li membranes. Such confined front‐growth processes are dominated by Li {110} or {200} growing faces, distinct from the root growth of single‐crystal Li dendrites outside the nanotubes. Controlled experiments show that high lithiophilicity/permeability, enabled by sufficient nitrogen/oxygen doping or pre‐lithiation, is critical for the stable encapsulation of lithium inside carbonaceous nanocapsules. First‐principles‐based calculations reveal that N/O doping can reduce the diffusion barrier for Li+ penetration, and facilitate Li filling driven by energy minimization associated with the formation of low‐energy Li/C interfaces.
Self‐Catalytic Small Interfering RNA Nanocarriers for Synergistic Treatment of Neurodegenerative Diseases
Authors: Ji Weihong , Li Yan , Peng Huan , Zhao Ruichen , Shen Jie , Wu Yanyue , Wang Jianze , Hao Qiulian , Lu Zhiguo , Yang Jun , Zhang Xin 
Published: October 2021

AbstractGene therapy has shown great potential for neurodegenerative diseases with complex pathology. However, its therapeutic effect is limited due to the delivery barriers and its own single function. Herein, self‐catalytic small interfering RNA (siRNA) nanocarriers (S/Ce‐PABMS) are developed to catalyze delivery process and treatment process for synergistic treatment of neurodegenerative diseases. On the one hand, the rough surface of the S/Ce‐PABMS mediated by ceria (CeO2) nanozymes can catalyze cellular uptake in the delivery process, so that S/Ce‐PABMS with acetylcholine analogs penetrate the blood–brain barrier and enter neurons more effectively. On the other hand, the CeO2 nanozymes can catalyze the treatment process by scavenging excess reactive oxygen species, and cooperate with siRNA‐targeting SNCA to decrease the α‐synuclein (α‐syn) aggregation and alleviate the Parkinsonian pathology. Moreover, the S/Ce‐PABMS treatment reduces the number of activated microglia and regulates the release of inflammatory cytokine, thereby relieving neuroinflammation. After treatment with S/Ce‐PABMS, dyskinesia in Parkinson's disease model mice is significantly alleviated. The finding shows that the self‐catalytic nanocarriers, S/Ce‐PABMS, have great potential in the treatment of neurodegenerative diseases.
3D Printing Scaffold Vaccine for Antitumor Immunity
Authors: Zhang Yue , Xu Jialu , Fei Ziying , Dai Huaxing , Fan Qin , Yang Qianyu , Chen Yitong , Wang Beilei , Wang Chao 
Published: October 2021

AbstractCancer vaccine platform has attracted great interest in the field of cancer immunotherapy. Here, 3D printed scaffolds loaded with immunoregulators are developed for enhanced cancer immunotherapy. The rapid manufacturing and precise molding based on 3D printing can realize the mass manufacturing of cancer vaccines and personalized design. Meanwhile, compared to the traditional hydrogel, the 3D‐scaffold with porous structure endows its similar functions compared with real lymphoid organs by recruitment of a great number of immune cells, leading to the formation of “artificial tertiary lymphoid structures,” where there is a promising site to enhance both humoral and cellular immune responses. Efficient anticancer immunity is induced when combined with immune checkpoint blockade to inhibit the tumor growth. Personalized antitumor scaffold vaccines are further demonstrated for filling of tumor site after surgery to prevent cancer metastasis. Taken together, these results promise the 3D printing scaffold vaccine as the potential strategy for cancer vaccine therapy in the future.
Whose responsibility? Reexamining Victorian justice system responses to the children and families of parents in prison
Authors: Hart Aaron , Field‐Pimm Melanie 
Published: October 2021

AbstractResearch into the children and families affected by parental imprisonment has demonstrated a range of well‐being concerns and proposed a comprehensive array of policy and practice responses, but little practical change has been achieved. With a focus on the Victorian justice system, we provide an overview of the literature and the current service provision, investigating why the policy inertia has persisted. Using feminist theory on the ethics and practices of care, we reexamine the findings of two significant Australian studies into the child and family‐centeredness of professionals within the police, courts, prisons and child protection agencies. We demonstrate that, at best, care is fragile and rendered from the margins of roles that are designed to be care‐less in relation to this cohort. At worst, justice system personnel and procedures can be resolutely uncaring. We suggest that reluctance among policymakers to displace attention from “core business” may explain the absence of policies to care for children and families of imprisoned parents, and highlight two further strategies: leadership to protect and extend the fragile care practice within the justice system; and new mandates to care for children and families of imprisoned parents extending from family‐oriented human services outside the justice system.
Dynamic electrical and mechanical properties of epoxy/silver nanowires composites
Authors: Bedel Vincent , Lonjon Antoine , Dantras Éric , Bouquet Michel , Lacabanne Colette 
Published: October 2021

AbstractThe aim of this work is to study electrically conductive composites from an epoxy matrix loaded with silver nanowires (AgNWs). Dynamic mechanical analysis (DMA) and dynamic dielectric spectroscopy (DDS) were used to study the molecular mobility of the epoxy matrix and its epoxy/AgNWs composites. The influence of the epoxy/amine ratio on the macroscopic properties was analyzed. The loss modulus of the epoxy matrix highlights two major relaxations, and . The relaxation is due to the localized mobility initiated by hydroxyl groups. The trelaxation is the anelastic effect liberated at the glass transition. The introduction of a small amount of AgNWs (<8 vol%) has practically no influence on the mechanical properties of the glassy state. For the rubbery state, AgNWs cause an increase in the storage modulus as a function of the filler content. The loss modulus profile is modified: The relaxation due to heterogeneities of the 3D network is observed independently from the hydration level of composites. The magnitude of the mode is increased due to the stick–slip phenomenon. Moreover, it is shifted toward lower temperatures upon the introduction of AgNWs. In other words, the activation enthalpy decreases for both modes. Electrical study through DDS and conductivity measurement of epoxy/AgNWs composites shows the consequence of a lower relative degree of conversion caused by the insertion of AgNWs. Epoxy/AgNWs composites allow us to obtain one of the highest values of the electrical conductivity for polymer‐based composites without any drastic evolution of mechanical properties.
Unprecedented Multicomponent Reaction of Indoles, CS2 and Nitroarenes: Stereoselective Synthesis of (Z)‐3‐((Arylamino)methylene)indoline‐2‐thiones
Authors: Qin Qi , Liu Xin‐Lian , Ma Ai‐Jun , Zhang Xiang‐Zhi , Peng Jin‐Bao 
Published: October 2021

AbstractAn efficient method for the stereoselective synthesis of (Z)‐3‐((arylamino)methylene)indoline‐2‐thiones have been developed via a novel multicomponent reaction of indoles, CS2 and nitroarenes. A range of functionalized indoline‐2‐thiones were prepared in moderate to good yields from easily available starting materials. The indoline‐2‐thione products can be easily derivatized to give biologically active thieno[2,3‐b]indole and thiopyrano[2,3‐b]indole skeletons in high yields.