bluuebunny/crossref_metadata_2025_split huggingface dataset:
SPOP Suppresses Hepatocellular Carcinoma Growth and Metastasis by Ubiquitination and Proteasomal Degradation of TRAF6
Authors: Chang Wenyi , Feng Kaiying , Zhou Peng , Gong Deao , Wang Ke , Huang Ailong , Wang Kai , Tang Ni 
Published: February 2025

ABSTRACTTumor necrosis factor receptor‐associated factor‐6 (TRAF6) is a well‐established upstream regulator of the IKK complex, essential for the modulation of the NF‐κB (nuclear factor kappa B) signaling pathway. Aberrant activation of TRAF6 has been strongly implicated in the pathogenesis of various cancers, including hepatocellular carcinoma (HCC). The speckle type BTB/POZ protein (SPOP), an E3 ubiquitin ligase substrate‐binding adapter, constitutes a significant component of the CUL3/SPOP/RBX1 complex, which is closely linked to tumorigenesis. In this study, we demonstrated that the E3 ubiquitin ligase SPOP shielded TRAF6 from proteasomal degradation, leading to the hyperactivation of the NF‐κB pathway. Notably, a liver cancer‐associated S119N mutation in SPOP resulted in a failure to mediate the ubiquitination and subsequent degradation of TRAF6. Moreover, both gain‐of‐function and loss‐of‐function experiments revealed that SPOP inhibits the proliferation and invasion of HCC cells through the TRAF6‐NF‐κB axis in vitro and in vivo. Taken together, our findings elucidate the underpinning mechanism by which SPOP negatively regulates the stability of the TRAF6 oncoprotein, thus offering a new therapeutic target for HCC intervention.
Learning and Teaching in the Era of Generative Artificial Intelligence Technologies: An In‐Depth Exploration Using Multi‐Analytical SEM‐ANN Approach
Authors: Shahzad Muhammad Farrukh , Xu Shuo , An Xin , Zahid Hira , Asif Muhammad 
Published: February 2025

ABSTRACTThe arrival of generative artificial intelligence (GAI) technologies marks a significant transformation in the educational landscape, with implications for teaching and learning performance. These technologies can generate content, simulate interactions, and adapt to learners' needs, offering opportunities for interactive learning experiences. In China's education sector, incorporating GAI technologies can address educational challenges, enhance teaching practices, and improve performance. This study scrutinises the impact of GAI technologies on learning performance in the education sector, focusing on the mediating roles of e‐learning competence (EC), desire for learning (DL), and beliefs about the future (BF), as well as the moderating role of facilitating conditions amongst Chinese educators. Data was collected from 411 teachers across various educational institutions in China using purposive sampling. PLS‐SEM and ANN were employed to assess the suggested structural model. The study results indicate that GAI technologies significantly influence learning performance by mediating EC, DL, and BF roles. Furthermore, facilitating conditions positively moderate the association amongst GAI technologies and EC, DL, and BF. This study underscores the critical role of self‐determination theory in shaping the effective incorporation of GAI technologies in education, offering valuable insights to improve teaching and learning outcomes in the Chinese education sector.
Body Mass–Biomass Scaling Modulates Species Keystone‐Ness to Press Perturbations
Authors: Li Xiaoxiao , Yang Wei , Novak Mark , Zhao Lei , de Ruiter Peter C. , Yang Zhifeng , Guill Christian 
Published: February 2025

ABSTRACTIdentifying species with disproportionate effects on other species under press perturbations is essential, yet how species traits and community context drive their ‘keystone‐ness’ remain unclear. We quantified keystone‐ness as linearly approximated per capita net effect derived from normalised inverse community matrices and as non‐linear per capita community biomass change from simulated perturbations in food webs with varying biomass structure. In bottom‐heavy webs (negative relationship between species' body mass and their biomass within the web), larger species at higher trophic levels tended to be keystone species, whereas in top‐heavy webs (positive body mass to biomass relationship), the opposite was true and the relationships between species' energetic traits and keystone‐ness were weakened or reversed compared to bottom‐heavy webs. Linear approximations aligned well with non‐linear responses in bottom‐heavy webs, but were less consistent in top‐heavy webs. These findings highlight the importance of community context in shaping species' keystone‐ness and informing effective conservation actions.
Portable ultra‐low‐field magnetic resonance imaging enables postictal seizure imaging
Authors: Bauer Tobias , Sabir Hemmen , Baumgartner Tobias , Rácz Attila , Pukropski Jan , Badr Mostafa , Olbrich Simon , Lange Annalena , Bisten Justus , Groteklaes Anne , Lehnen Nils C. , Cendes Fernando , Radbruch Alexander , Surges Rainer , Rüber Theodor 
Published: February 2025

AbstractThe detection of transient peri‐ictal magnetic resonance imaging (MRI) abnormalities has been variable after epileptic seizures. The most common reason for this variability is that abnormalities may disappear if the interval between seizure and scan acquisition is prolonged using conventional high‐field systems. Here, we deployed a portable ultra‐low‐field MRI system in the presurgical evaluation at the bedside of individuals with epilepsy. We hypothesized that this novel technology enables rapid postictal scans and reliably shows focal peri‐ictal MRI abnormalities in the seizure onset zone. A .064‐T Swoop Portable MR Imaging System was used. Postictally, an axial diffusion‐weighted sequence was acquired. The interictal MRI consisted of the diffusion‐weighted and three‐dimensional T1‐weighted sequences. Postictal–interictal difference maps of diffusion‐weighted volumes were calculated. Three individuals were included. Two individuals with focal aware seizures scanned 29 s and 19 min after the seizure, respectively, showed focal restrictions in diffusivity in the seizure onset zone, and a third individual scanned 5 h 45 min after a focal to bilateral tonic–clonic seizure showed global restrictions of diffusivity. Portable ultra‐low‐field MRI opens a new line of inquiry with the aim to establish postictal seizure imaging as part of the presurgical evaluation of people with epilepsy.
Cyclic Mechanical Behavior, Damage‐Coupled Constitutive Model, and Remaining Life Prediction Model of QCr0.8 at High Temperature
Authors: Wang Jundong , Yu Jiaheng , Wen Zhixun , Xu Xiangqian , Shi Yanqi , Yue Zhufeng 
Published: February 2025

ABSTRACTThe low cycle fatigue (LCF) behavior including the fatigue life, hysteresis loop, and damage process of QCr0.8 at 350°C and 500°C was studied. A viscoplastic damage‐coupled unified creep‐plasticity constitutive model was developed to simulate the cyclical mechanical behavior and damage of QCr0.8 with the same set of parameters at identical temperatures. Meanwhile, LCF damage measurement methods based on changes in elastic modulus and evolution of peak stress were compared. To assess the remaining life of QCr0.8 after a certain period of service, a remaining life prediction model based on the entropy increase theory within the unified thermodynamic framework was proposed. The results show that the prediction results are in good agreement with experimental data.
Synthesis of a new amino acid derivative with long‐lasting hair shape control effects and elucidation of its mechanisms
Authors: Sato Sotaro , Tabuchi Hinako , Katayama Kohtaro , Matsumoto Kenji , Miyamoto Hiroyuki , Kanno Sorai , Sekiguchi Hiroshi , Tanaka Yoshinobu , Inoue Mari , Murata Choji , Hotta Hiroki , Tsujino Yoshio 
Published: February 2025

AbstractObjectiveHair is a crucial aspect of an individual's physical appearance. Thus, there is always a need for technology to care for curls and undulations. In this study, we found that heat treatment with a mixed aqueous solution of cysteamine (2‐aminoethanethiol) and fumaric acid was highly effective in improving hair quality. The purpose of this study was to scientifically elucidate the effectiveness of this technology by identifying its active ingredients, evaluating their functionality and analysing changes in the internal structure of hair after treatment.MethodWe observed changes in hair shape through a hair straightening test, analysed the components using liquid chromatography/mass spectrometry (LC/MS) and investigated the effects on mechanical properties using bending and surface friction measurements. Furthermore, we analysed the hair conditions using small‐angle X‐ray scattering (SAXS) measurements and evaluated water retention using thermal analysis.ResultsIn the hair straightening test, even extremely strong curls of African human hair improved. The LC/MS results revealed that the active ingredient was 2‐(2‐aminoethylthio) succinic acid (ATS). Furthermore, by treating hair with ATS, decreases in the coefficient of friction and bending stiffness and an increase in moisture content were observed. The SAXS measurements revealed that the treatment widened the distance between the intermediate filaments (IFs) inside the hair and improved the orientation of the IFs.ConclusionATS, produced by the thiol–ene reaction between fumaric acid and cysteamine, acts as an active ingredient in hair shape control. ATS reacted within the matrix and increased the IF–IF distance. This suggests that ATS functions as a crosslinker for keratin proteins. ATS is believed to increase the moisture content of hair and improve hair texture.
TAXATION OF TOP INCOMES AND TAX AVOIDANCE
Authors: Di Nola Alessandro , Kocharkov Georgi , Scholl Almuth , Tkhir Anna‐Mariia , Wang Haomin 
Published: February 2025

AbstractWe examine the aggregate and distributional effects of raising the top marginal income tax rate in the presence of entrepreneurial tax avoidance. In a model with heterogeneous agents and occupational choice, entrepreneurs can avoid taxes by choosing the legal form of business organization and shifting income across tax bases. Applied to the U.S. economy, we find that tax avoidance weakens the distortionary effects of higher income taxes at the top but makes them ineffective at lowering inequality. Eliminating tax avoidance by an equal tax treatment of entrepreneurs across all legal forms substantially increases tax revenue, aggregate output, and welfare.
Validation of the computerised battery for neuropsychological evaluation of children (BENCI) in a Cuban sample
Authors: Pérez‐Marfil María Nieves , Fernández‐Alcántara Manuel , Navarro Elena , García‐Navarro Xiomara , Cruz‐Quintana Francisco 
Published: February 2025

AbstractThe aim of this research was to analyse the reliability and validity of the Computerised Battery for Neuropsychological Evaluation of Children (BENCI) in a Cuban population of children and adolescents. The study involved 1714 Cuban students between the ages of 6 and 18 who were divided into three groups according to their level of education (Elementary: 6–11 years old; High School: 12–14 years old; and Pre‐University: 15–18 years old). All participants were evaluated using the BENCI with some also undergoing additional neuropsychological testing. The BENCI evaluates the following cognitive domains: processing speed, visuomotor coordination, attention, memory, language, and executive functions. The results showed that the BENCI has good test–retest reliability indices and high internal consistency values in Reasoning, Reaction Time, and Working Memory. In terms of validity, the data revealed significant correlations between the BENCI tests and other neuropsychological tests assessing similar cognitive functions. The BENCI also has discriminative validity, as it was found that performance on the tests varies according to the level of education of those being evaluated. In terms of construct validity, confirmatory factor analysis showed that the fit indices for the executive functions dimension of the BENCI are adequate. The data show that the BENCI is a reliable and valid instrument for assessing neurodevelopment in Cuban children and adolescents. Since this is the first neuropsychological test of its kind to be validated in the Cuban population, this finding is of particular importance.
Factors associated with multiple‐death suicides among rural US decedents
Authors: Soupene Victor A. , Hays Timothy , Davis Jonathan , Vakkalanka J. Priyanka 
Published: February 2025

AbstractPurposeTo identify contributing circumstances to multiple‐death suicides in rural US counties from law enforcement and coroner/medical examiner narratives.MethodsWe identified multiple‐death suicides as multiple suicides (i.e., two or more decedents) from the National Violent Death Reporting System (NVDRS) between 2013 and 2021. We identified rural decedents from their residence in NVDRS using federal information processing codes linked to Rural–Urban Continuum Codes. Quantitatively, we described demographic characteristics and circumstances leading to the suicide. From narrative law enforcement and coroner/medical examiner reports, we generated codes describing demographic and circumstance information using Dedoose software. We then used inductive content analysis to identify themes related to multiple‐death suicides.FindingsAmong all multiple‐death suicides (n = 50 multiple‐death suicides, 99 suicide decedents), decedents were mostly non‐Hispanic White (n = 90; 91%), were male (n = 54; 54%), and had a high school diploma or less (n = 51; 56%). We identified four themes: meticulous and considerate planning (e.g., with notes, reasons, and next steps), mutual dependency (e.g., decedents were in separable), unbearable health conditions (e.g., pain, poor quality of life from chronic illnesses), and social factors of despair (e.g., financial strain, legal problems, and interpersonal violence).ConclusionDeveloping strategies for discussing suicidal ideation and improving access to financial resources and health care may reduce multiple‐death suicides in the rural United States, particularly among older adults with chronic health problems. Improving other public health initiatives such as interpersonal violence and chronic disease prevention and management may further prevent multiple‐death suicides.
Patient perspectives of the impact of a rural health center closure: A cross‐sectional survey
Authors: Trombetta Mallory , Suchy Natalea , Irwin Adriane N. 
Published: February 2025

AbstractPurposeTo describe the impact from the closure of a rural community's only health center on chronic health conditions, access to care, and quality of care received from the patient's perspective.MethodsThis was a cross‐sectional telephone survey. Adult patients established with a rural health center participated in a 19‐item survey at 6 months post‐closure to collect data on perceived impacts. Data were summarized using descriptive statistics.FindingsThere were 249 patients contacted, with 131 participants (52.6% response rate). Participants had a median age of 63 years (interquartile range, 44.5–73.0), and the majority were female (n = 82; 62.6%) and had been established with the health center for over 10 years (n = 79; 60.3%). At 6 months, the majority of participants had established care with another health center (n = 91; 69.5%). Most participants felt that the closure made it more difficult to access care (n = 106; 80.9%) but did not feel the closure reduced the quality of care they were receiving (n = 42; 32.1%). There was no impact perceived on the management of most health conditions, except chronic pain where worsening was the most selected option.ConclusionsPatients were able to successfully transition care after closure of their local health center, and most did not perceive an impact on the quality of care received. However, participants reported reduced access to care. Future research might repeat this process, perhaps using a mixed‐method approach, to intentionally capture nuances in patient behavior, experiences, and attitudes following the loss of primary care services in rural communities.
CCL19+ Fibroblasts Promote Tertiary Lymphoid Structure in Oral Lichen Planus: A Retrospective Study
Authors: Lai Yi‐Rao , Pan Lei , Jiang Xin‐Ke , Deng Yi‐Wen , Feng Ming‐Hua , Li Jin , Han Xiao‐Zhe , Sun Lei , Wang Yu‐Feng , Tang Guo‐Yao 
Published: February 2025

ABSTRACTObjectivesThe priming mechanism of adaptive immunity in oral mucositis remains unclarified. This study aimed to elucidate the mechanisms of oral lichen planus (OLP), with a specific focus on the role of inducible tertiary lymphoid structures (iTLSs) and stromal‐immune microenvironments within OLP.Materials and MethodsWe employed single‐cell RNA sequencing and multiplex immunofluorescence staining to characterize the spatial characteristics and transcriptional signature of iTLSs in OLP. To investigate whether the presence of iTLSs influences the disease severity of OLP, we retrospectively collected clinical data from OLP patients.ResultsBased on single‐cell resolution analysis, we identified distinct cellular compositions and fibroblast‐immune cells interactions within the iTLSs of OLP. We highlighted the critical role of CCL19+ fibroblasts in T‐cell chemotaxis, promoting the development of iTLSs. Lymphotoxin was demonstrated to activate fibroblasts expressing CCL19. We revealed a significant correlation between the presence of iTLSs and increased severity of OLP, marked by more atrophic‐erosive forms and higher disease scores in patients with iTLSs.ConclusionsThe present research proposed the fibroblast‐immune cell interactions within iTLSs as essential factors in OLP's inflammatory milieu, indicating iTLSs as the priming structure of T‐cell immunity in the local oral mucosa.
Deciphering Disulfidptosis‐Linked lncRNA Patterns as Potential HNSCC Biomarkers
Authors: Chen Qi , Shi Xiao , Bao Yuanyuan , Chen Yue 
Published: February 2025

ABSTRACTBackgroundOur investigation sought to uncover the intrinsic features of Head and Neck Squamous Cell Carcinoma (HNSCC), particularly the role of long non‐coding RNAs implicated in disulfidptosis (DRLs).Materials and MethodsWe carried out lncRNA‐mRNA RNA‐Seq studies on HNSCC cells and harnessed the data from The Cancer Genome Atlas (TCGA), which includes 522 HNSCC tumors and 44 normal specimens. Bioinformatics evaluations aided in recognizing DRLs and estimating their prognostic value. Furthermore, we built a predictive model related to the chosen DRLs to scrutinize its linkage with the patients' prognosis. We also dug into tumor mutation loads and responses to chemotherapy.ResultsOur study identified three key DRLs (LINC02434, AC245041.2, and LINC02762) with considerable correlation to HNSCC prognosis. The risk model, utilizing these DRLs, successfully categorized patients into high‐risk and low‐risk clusters, uncovering differential survival trajectories. Moreover, the same risk model conveyed unique prognostic potential in HNSCC. Surveying the tumor microenvironment unfolded disparities between the groups, hinting toward potential implications for tactics in immunotherapy. We recognized distinct chemotherapeutic drugs with fluctuating responses across the risk clusters and molecular categories.ConclusionThis investigation not only sheds light on prospective therapeutic pathways but also enhances our grasp of the molecular intricacies of HNSCC.
Real‐World Study of Later‐Line Anlotinib in R/M Head and Neck Squamous Cell Carcinoma
Authors: Ju Houyu , Zhao Yuanyuan , Zhao Minqi , Chen Lingyan , Geng Xinrong , Xu Xuanli , Ren Guoxin , Hu Jingzhou 
Published: February 2025

ABSTRACTObjectivesThe option is rare for patients with recurrent or metastatic head and neck squamous cell carcinoma (R/M HNSCC) failed to prior‐line immune checkpoint inhibitors (ICIs) and cetuximab‐based treatment. The efficacy of salvage therapy was unsatisfied.Materials and MethodsWe collected the clinical data of R/M HNSCC patients progressed from prior‐line immunotherapy and cetuximab therapy, and retrospectively analyzed the efficacy and toxicity of anlotinib‐based therapy in these patients.ResultsIn total, 24 eligible participants were accrued between October 2021 and November 2024. Up to the cutoff time of November 1st, 2024, the objective response rate was 37.5%. Survival analysis revealed the median progression‐free survival and overall survival was 4.27 months (95% CI, 1.53–7.01 months) and 8.67 months (95% CI, 5.62–11.72 months), respectively. For the safety, most common TRAEs with any grades were hypertension, hand–foot syndrome, gastrointestinal response, hemorrhage, hepatic dysfunction, and fatigue. Grade 3 or more TRAEs were observed in 3 (12.5%) patients, and no Grade 5 TRAEs were occurred.ConclusionsOur observations indicated that anlotinib‐based therapy had considerable efficacy and well tolerance in R/M HNSCC patients failed to ICIs and cetuximab‐based therapy, and might be acted as a novel and potentially effective option in later‐line treatment of R/M HNSCC.
Unravelling the metabolic signatures and associated pathways underlying saline‐alkali stress resilience in the halophyte Salvadora persica
Authors: Kumari Asha , Fatnani Dhara , Seth Chandra Sekhar , Parida Asish Kumar 
Published: February 2025

AbstractSoil salinization and alkalization are the foremost abiotic stresses adversely affecting plant growth and yield worldwide. Salvadora persica is an important facultative halophyte that has been reported for its high salinity tolerance ability. The present study undertakes the growth, photosynthetic efficiency, chlorophyll fluorescence, antioxidative defense, ionomic adjustments, and metabolomic responses of S. persica to understand its resilience mechanisms under saline‐alkali stress. Our findings reveal that S. persica undergoes substantial physiological, ionomic, and metabolic modulations for endurance under saline‐alkali stress. Under saline‐alkali stress, metabolomic analysis identified 75 differentially accumulated metabolites (DAMS), including amino acids, sugars, sugar alcohols, organic acids, polyphenols, and phytohormones. Notable upregulated metabolites included quercetin (+4‐fold), naringenin (+2.6‐fold), glycerol (+2.4‐fold), and proline (+1.7‐fold), whereas D‐mannitol (−3.8‐fold), D‐talose (−2.9‐fold), and GA3 (−2.8‐fold) were significantly downregulated. Pathway enrichment analysis revealed alterations in key metabolic pathways, such as amino acid metabolism, starch and sucrose metabolism, tricarboxylic acid cycle (TCA cycle), glycolysis/gluconeogenesis, and photosynthetic carbon fixation. A 2‐fold reduction in glucose indicated an enhanced glycolytic flux to support energy needs under stress. The study highlights S. persica's adaptive strategy of slower nitrogen metabolism, increased glycolysis, and downregulated TCA cycle, giving an indication towards a slower growth rate under saline‐alkali stress to reallocate energy for osmolyte biosynthesis. The amino acids, sugars, and sugar alcohols emerged as major contributors to osmotic adjustment. A robust antioxidant defense system exists in S. persica to mitigate ROS under saline‐alkali stress. These findings provide insights into the mechanisms of saline‐alkali tolerance in the halophyte S. persica, offering valuable direction for developing saline‐alkali‐tolerant crops.