bluuebunny/crossref_metadata_2025_split huggingface dataset:
Do Archive Catalogues Make History?: Exploring Interactions between Historians and Archives
Authors: Dunley Richard , Pugh Jo 
Published: July 2021

Abstract
Archival research is foundational to the writing of most works of academic history and is a central part of the professional identity of historians. Despite this, we know very little about what historians do in archives, and how it shapes the process of writing history. This article uses quantitative data from The National Archives of the UK to analyse one part of this puzzle, namely how historians choose what to look at in an archive. It explores the nature of archive catalogues, and their relationship to the collections they describe. It goes on to assess how catalogues shape what historians choose to view when they visit an archive, and concludes by looking more widely at historians’ information seeking practices. Through this analysis the article will argue that external factors have a far greater impact on archival research, and through it the writing of history, than is traditionally acknowledged, and as such historians need to pay considerably more attention to the ways in which their access to archives is shaped by these factors.
‘Look into Their Hearts’ 7-Year-Old Boy with Sickle Cell Disease, Fever, Left Sixth and Seventh Cranial Nerve Palsy and Left Hemiplegia
Authors: Jabateh Sekou M , Mohsenibod Hadi 
Published: July 2021

ABSTRACT
A 7-year-old Liberian boy presented with fever, severe headache, neck pain and ‘not being able to walk’. In the emergency room, his sickle cell screen was positive, and his haemoglobin level was 7 g/dl. Initially, he was admitted to the ward with a diagnosis of sickle cell painful crisis and was treated with ceftrixone iv, and oral morphine and paracetamol. In a more complete physical examination, he had left peripheral seventh nerve palsy, left sixth nerve palsy and ipsilateral hemiplegia, also neck rigidity. In a bedside ultrasound scan, he had a large, mobile vegetation on the aortic leaflet of the mitral valve. The final diagnosis was acute infective endocarditis with multiple embolic strokes in a child with sickle cell disease. Headache and neck rigidity was most likely due to SAH or meningitis.
Long-term Survival after Combined Epidural–General Anesthesia or General Anesthesia Alone: Follow-up of a Randomized Trial
Authors: Du Ya-Ting , Li Ya-Wei , Zhao Bin-Jiang , Guo Xiang-Yang , Feng Yi , Zuo Ming-Zhang , Fu Cong , Zhou Wei-Jie , Li Huai-Jin , Liu Ya-Fei , Cheng Tong , Mu Dong-Liang , Zeng Yuan , Liu Peng-Fei , Li Yan , An Hai-Yan , Zhu Sai-Nan , Li Xue-Ying , Li Hui-Juan , Wu Yang-Feng , Wang Dong-Xin , Sessler Daniel I. ,   for the Peking University Clinical Research Program Study Group
Published: July 2021

Background
Experimental and observational research suggests that combined epidural–general anesthesia may improve long-term survival after cancer surgery by reducing anesthetic and opioid consumption and by blunting surgery-related inflammation. This study therefore tested the primary hypothesis that combined epidural–general anesthesia improves long-term survival in elderly patients.

Methods
This article presents a long-term follow-up of patients enrolled in a previous trial conducted at five hospitals. Patients aged 60 to 90 yr and scheduled for major noncardiac thoracic and abdominal surgeries were randomly assigned to either combined epidural–general anesthesia with postoperative epidural analgesia or general anesthesia alone with postoperative intravenous analgesia. The primary outcome was overall postoperative survival. Secondary outcomes included cancer-specific, recurrence-free, and event-free survival.

Results
Among 1,802 patients who were enrolled and randomized in the underlying trial, 1,712 were included in the long-term analysis; 92% had surgery for cancer. The median follow-up duration was 66 months (interquartile range, 61 to 80). Among patients assigned to combined epidural–general anesthesia, 355 of 853 (42%) died compared with 326 of 859 (38%) deaths in patients assigned to general anesthesia alone: adjusted hazard ratio, 1.07; 95% CI, 0.92 to 1.24; P = 0.408. Cancer-specific survival was similar with combined epidural–general anesthesia (327 of 853 [38%]) and general anesthesia alone (292 of 859 [34%]): adjusted hazard ratio, 1.09; 95% CI, 0.93 to 1.28; P = 0.290. Recurrence-free survival was 401 of 853 [47%] for patients who had combined epidural–general anesthesia versus 389 of 859 [45%] with general anesthesia alone: adjusted hazard ratio, 0.97; 95% CI, 0.84 to 1.12; P = 0.692. Event-free survival was 466 of 853 [55%] in patients who had combined epidural–general anesthesia versus 450 of 859 [52%] for general anesthesia alone: adjusted hazard ratio, 0.99; 95% CI, 0.86 to 1.12; P = 0.815.

Conclusions
In elderly patients having major thoracic and abdominal surgery, combined epidural–general anesthesia with epidural analgesia did not improve overall or cancer-specific long-term mortality. Nor did epidural analgesia improve recurrence-free survival. Either approach can therefore reasonably be selected based on patient and clinician preference.

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Improving Vascular Access Dressing Integrity in the Acute Care Setting
Authors: DeVries Michelle , Sarbenoff Jill , Scott Nancy , Wickert Margaret , Hayes Lisa Marie 
Published: July 2021

PURPOSE:
The purpose of this quality improvement project was to evaluate transparent vascular access dressings and the use of a liquid gum mastic adhesive on improving dressing integrity over peripheral intravenous (PIV) insertion sites without increasing medical adhesive-related skin injuries (MARSIs) such as tears.

PARTICIPANTS AND SETTING:
A multidisciplinary team consisting of specialists in infection prevention, vascular access, nursing professional development, materials management, and WOC nurses met to review current audit data and available products to trial on 2 intermediate care units in our 2 hospitals in Indiana with a combined average daily unit census of 35 patients.

APPROACH:
Four dressing protocols—including our existing dressing with education, and an updated dressing with education, and the updated and new dressing, both with education and the addition of a gum mastic adhesive agent—were sequentially implemented by nurses on the units, each over a 2-week period. The goal was for 80% of the dressings to remain with all 4 corners fully intact without reinforcement at day 7, or sooner if PIV was discontinued before day 7. Data were reported as frequencies for intact dressings and skin complications.

OUTCOMES:
Education combined with the original dressing and the updated dressing did not achieve the goal of 80% fully intact dressings in the samples evaluated. The addition of the adhesive agent to the updated and new dressings with education exceeded the 80% goal. In addition, there were zero exposed PIV insertion sites and no documented MARSI in any of the 4 protocols.

IMPLICATIONS FOR PRACTICE:
We continued to collect postproject data of 30,049 vascular access sites including central line catheters and observed the same effectiveness of incorporating a gum mastic adhesive on dressing integrity. This practice change has now become standard of care in our institution.
One Year of SARS-CoV-2: How Much Has the Virus Changed?
Authors: Vilar Santiago , Isom Daniel G. 
Published: July 2021

AbstractSARS-CoV-2 coronavirus has caused a world-wide crisis with profound effects on both healthcare and the economy. In order to combat the COVID-19 pandemic, research groups have shared viral genome sequence data through the GISAID initiative. We collected and computationally profiled ∼223,000 full SARS-CoV-2 proteome sequences from GISAID over one year for emergent nonsynonymous mutations. Our analysis shows that SARS-CoV-2 proteins are mutating at substantially different rates, with most viral proteins exhibiting little mutational variability. As anticipated, our calculations capture previously reported mutations occurred in the first period of the pandemic, such as D614G (Spike), P323L (NSP12), and R203K/G204R (Nucleocapsid), but also identify recent mutations like A222V and L18F (Spike) and A220V (Nucleocapsid). Our comprehensive temporal and geographical analyses show two periods with different mutations in the SARS-CoV-2 proteome: December 2019 to June 2020 and July to November 2020. Some mutation rates differ also by geography; the main mutations in the second period occurred in Europe. Furthermore, our structure-based molecular analysis provides an exhaustive assessment of mutations in the context of 3D protein structure. Emerging sequence-to-structure data is beginning to reveal the site-specific mutational tolerance of SARS-CoV2 proteins as the virus continues to spread around the globe.
A cross-sectional study of the association between frequency of telecommuting and unhealthy dietary habits among Japanese workers during the COVID-19 pandemic
Authors: Kubo Yoshiko , Ishimaru Tomohiro , Hino Ayako , Nagata Masako , Ikegami Kazunori , Tateishi Seiichiro , Tsuji Mayumi , Matsuda Shinya , Fujino Yoshihisa 
Published: July 2021

2.Abstract and keywordsObjectiveDue to the COVID-19 pandemic, telecommuting has become a new way of working that has not only changed individuals’ work, but also their health and lifestyle. We examined the relationship between telecommuting frequency and unhealthy dietary habits among Japanese workers.MethodsA total of 33,302 workers completed an Internet survey about telecommuting and dietary habits. Data from 13,468 office workers who telecommuted were analyzed. Telecommuting frequency during the COVID-19 pandemic was extracted from a questionnaire. The odds ratios (ORs) of four types of dietary habits, namely, skipping breakfast, solitary eating, lower meal frequency, and meal substitution associated with telecommuting frequency were estimated using multilevel logistic regression nested in the prefecture of residence to control for differences in residential area.ResultsThe multivariate OR of skipping breakfast was 1.15 (95% CI: 1.03–1.29, p=0.013) for participants who telecommuted in excess of four days per week compared to those who rarely telecommuted. Similarly, the OR of solitary eating, lower meal frequency and meal substitution were 1.44 (95%CI: 1.28–1.63, p<0.001), 2.39 (95%CI: 1.66–3.44, p<0.001), and 1.26 (95%CI: 1.04–1.51, p=0.015) for those who telecommuted in excess of four days per week compared to those who rarely telecommuted. There was a statistically significant increase in the dose-response trend in ORs of solitary eating (p for trend <0.001), lower meal frequency (p for trend <0.001), and meal substitution (p for trend =0.001) with increasing telecommuting frequency.ConclusionTelecommuters may develop unhealthy dietary habits, indicating the need for strategies to help telecommuters manage their nutrition and diet.
Molecular pathways in post-colonoscopy versus detected colorectal cancers: results from a nested case-control study
Authors: Bogie Roel M.M. , le Clercq Chantal M.C. , Voorham Quirinus J.M. , Cordes Martijn , Sie Daoud , Rausch Christian , van den Broek Evert , de Vries Sara D.J. , van Grieken Nicole C.T. , Riedl Robert G. , Sastrowijoto Prapto , Speel Ernst-Jan , Vos Rein , Winkens Bjorn , van Engeland Manon , Ylstra Bauke , Meijer Gerrit A. , Masclee Ad A.M. , Carvalho Beatriz 
Published: July 2021

AbstractBackgroundPost-colonoscopy colorectal cancers (PCCRCs) pose a challenge in clinical practice. PCCRCs occur due to a combination of procedural and biological causes. Specific features of lesions may contribute for this. In a nested case-control study, we compared clinical and molecular features of PCCRCs and detected CRCs (DCRCs).MethodsPCCRCs were defined according to the WEO 2018 classification, as cancers occurring after a complete index colonoscopy, which excluded CRC. CRCs in patients without colonoscopy or with colonoscopy >10 years before were defined as DCRCs. Whole genome chromosomal copy number changes and mutation status of genes commonly affected in CRC (including APC, KRAS, BRAF, FBXW7, PIK3CA, NRAS, SMAD4 and TP53) were examined by low-coverage WGS and targeted sequencing, respectively. MSI and CIMP status were also determined.ResultsIn total, 122 PCCRCs and 98 DCRCs with high quality DNA were examined. PCCRCs were more often located proximally in the colon (p<0.001), non-polypoid appearing (p=0.004), early stage (p=0.009), and poorly differentiated (p=0.006). PCCRCs showed similar patterns of DNA copy number changes typical of CRC, although significantly less 18q loss (FDR <0.2), compared to DCRCs. No significant differences in mutations were detected between PCCRCs and DCRCs. PCCRCs were more commonly CIMP-high (p=0.014) and MSI (p=0.029). After correction for tumour location, the only molecular difference between PCCRCs and DCRCs that remained significant was less frequent loss of 18q chromosome in PCCRCs (p=0.005).ConclusionAlthough PCCRCs show molecular characteristics that are common to the canonical CIN, MSI and hypermethylation pathways, molecular features associated with the sessile serrated lesions (SSLs) and non-polypoid colorectal neoplasms (CRNs) are more commonly seen in PCCRCs than in DCRCs. This and the clinical features observed in PCCRCs support the hypothesis that sessile serrated lesions and non-polypoid CRNs are contributors to the development of these cancers. In order to further reduce the occurrence of PCCRCs, the focus should be directed at improving the detection, determination and endoscopic removal of these non-polypoid CRN and SSLs.Clinical Trial RegistrationNTR3093 in the Dutch trial register (www.trialregister.nl)
Dimeric IgA is a specific biomarker of recent SARS-CoV-2 infection
Authors: Drummer Heidi E , Van Huy , Klock Ethan , Zheng Shuning , Wei Zihui , Boo Irene , Center Rob J , Li Fan , Bhat Purnima , Ffrench Rosemary , Lau Jillian SY , McMahon James , Laeyendecker Oliver , Fernandez Reinaldo E. , Manabe Yukari C. , Klein Sabra L. , Quinn Thomas C. , Anderson David A. 
Published: July 2021

AbstractCurrent tests for SARS-CoV-2 antibodies (IgG, IgM, IgA) cannot differentiate recent and past infections. We describe a point of care, lateral flow assay for SARS-CoV-2 dIgA based on the highly selective binding of dIgA to a chimeric form of secretory component (CSC), that distinguishes dIgA from monomeric IgA. Detection of specific dIgA uses a complex of biotinylated SARS-CoV-2 receptor binding domain and streptavidin-colloidal gold. SARS-CoV-2-specific dIgA was measured both in 112 cross-sectional samples and a longitudinal panel of 362 plasma samples from 45 patients with PCR-confirmed SARS-CoV-2 infection, and 193 discrete pre-COVID-19 or PCR-negative patient samples. The assay demonstrated 100% sensitivity from 11 days post-symptom onset, and a specificity of 98.2%. With an estimated half-life of 6.3 days, dIgA provides a unique biomarker for the detection of recent SARS-CoV-2 infections with potential to enhance diagnosis and management of COVID-19 at point-of-care.
Natural and after colon washing fecal samples: the two sides of the coin for investigating the human gut microbiome
Authors: Piancone Elisabetta , Fosso Bruno , De Robertis Mariangela , Notario Elisabetta , Oranger Annarita , Manzari Caterina , Marzano Marinella , Bruno Silvia , D’Erchia Anna Maria , Maio Dominga , Minelli Martina , Vergallo Ilaria , Minelli Mauro , Pesole Graziano 
Published: July 2021

ABSTRACTTo date there are several studies focusing on the importance of gut microbiome for human health, however the selection of a universal sampling matrix representative of the microbial biodiversity associated to the gastrointestinal (GI) tract, still represents a challenge. Here we present a study in which, through a deep metabarcoding analysis of the 16S rRNA gene, we compared two sampling matrices, feces (F) and colonic lavage liquid (LL), in order to evaluate their accuracy to represent the complexity of the human gut microbiome. A training set of 37 volunteers was attained and paired F and LL samples were collected from each subject. A preliminary absolute quantification of total 16S rDNA, performed by droplet digital PCR (ddPCR), confirmed that sequencing and taxonomic analysis were performed on same total bacterial abundance obtained from the two sampling methods. The taxonomic analysis of paired samples revealed that, although specific taxa were predominantly or exclusively observed in LL samples, as well as other taxa were detectable only or were predominant in stool, the microbiomes of the paired samples F and LL in the same subject hold overlapping taxonomic composition. Moreover, LL samples revealed a higher biodiversity than stool at all taxonomic ranks, as demonstrated by the Shannon Index and the Inverse Simpson’s Index. We also found greater inter-individual variability than intra-individual variability in both sample matrices. Finally, functional differences were unveiled in the gut microbiome detected in the F and LL samples. A significant overrepresentation of 22 and 13 metabolic pathways, mainly occurring in Firmicutes and Proteobacteria, was observed in gut microbiota detected in feces and LL samples, respectively. This suggests that LL samples may allow for the detection of microbes adhering to the intestinal mucosal surface as members of the resident flora that are not easily detectable in stool, most likely representative of a diet-influenced transient microbiota. This first comparative study on feces and LL samples for the study of the human gut microbiome demonstrates that the use of both types of sample matrices may represent a possible choice to obtain a more complete view of the human gut microbiota in response to different biological and clinical questions.
B1-type cyclins control microtubule organization during cell division in Arabidopsis
Authors: Motta Mariana R. , Zhao Xin’Ai , Pastuglia Martine , Belcram Katia , Roodbarkelari Farshad , Komaki Maki , Harashima Hirofumi , Komaki Shinichiro , Bulankova Petra , Heese Maren , Riha Karel , Bouchez David , Schnittger Arp 
Published: July 2021

AbstractFlowering plants contain a large number of cyclin families, each containing multiple members, most of which have not been characterized to date. Here, we analyzed the role of the B1 subclass of mitotic cyclins in cell cycle control during Arabidopsis development. While we reveal CYCB1;5 to be a pseudogene, the remaining four members were found to be expressed in dividing cells. Mutant analyses showed a complex pattern of overlapping, development-specific requirements of B1-type cyclins with CYCB1;2 playing a central role. The double mutant cycb1;1 cycb1;2 is severely compromised in growth, yet viable beyond the seedling stage, hence representing a unique opportunity to study the function of B1-type cyclin activity at the organismic level. Immunolocalization of microtubules in cycb1;1 cycb1;2 and treating mutants with the microtubule drug oryzalin revealed a key role of B1-type cyclins in orchestrating mitotic microtubule networks. Subsequently, we identified the GAMMA-TUBULIN COMPLEX PROTEIN 3-INTERACING PROTEIN 1 (GIP1/MOZART) as an in vitro substrate of B1-type cyclin complexes and further genetic analyses support an important role in the regulation of GIP1 by CYCB1s.
Conversion of cancer-associated fibroblasts from pro- to antitumor improves the sensitivity of pancreatic cancer to chemotherapeutics
Authors: Iida Tadashi , Mizutani Yasuyuki , Esaki Nobutoshi , Ponik Suzanne M. , Burkel Brian M , Weng Liang , Kuwata Keiko , Masamune Atsushi , Ishihara Seiichiro , Haga Hisashi , Kataoka Kunio , Mii Shinji , Shiraki Yukihiro , Ishikawa Takuya , Ohno Eizaburo , Kawashima Hiroki , Hirooka Yoshiki , Fujishiro Mitsuhiro , Takahashi Masahide , Enomoto Atsushi 
Published: July 2021

AbstractPrevious therapeutic attempts to deplete cancer-associated fibroblasts (CAFs) or inhibit their proliferation in pancreatic ductal adenocarcinoma (PDAC) were not successful in mice or patients. Thus, CAFs may be tumor suppressive or heterogeneous, with distinct cancer-restraining and -promoting CAFs (rCAFs and pCAFs, respectively). Here, we show that induced expression of the glycosylphosphatidylinositol-anchored protein Meflin, a rCAF-specific marker, in CAFs by genetic and pharmacological approaches improved the chemosensitivity of mouse PDAC. A chemical library screen identified Am80, a synthetic, non-natural retinoid, as a reagent that effectively induced Meflin expression in CAFs. Am80 administration improved the sensitivity of PDAC to chemotherapeutics, accompanied by increases in tumor vessel area and intratumoral drug delivery. Mechanistically, Meflin was involved in the suppression of tissue stiffening by interacting with lysyl oxidase to inhibit its collagen crosslinking activity. These data suggested that modulation of CAF heterogeneity may represent a strategy for PDAC treatment.
Time-dependent toxicity of tire particles on soil nematodes
Authors: Kim Shin Woong , Leifheit Eva F. , Maaß Stefanie , Rillig Matthias C. 
Published: July 2021

ABSTRACTTire-wear particles (TWPs) are being released into the environment by wearing down during car driving, and are considered an important microplastic pollution source. The chemical additive leaching from these polymer-based materials and its potential effects are likely temporally dynamic, since larger amounts of potentially toxic compounds can gradually increase with contact time of plastic particles with surrounding media. In the present study, we conducted soil toxicity tests using the soil nematode Caenorhabditis elegans with different soil pre-incubation (30 and 75 days) and exposure (short-term exposure, 2 days; lifetime exposure, 10 days) times. Soil pre-incubation increased toxicity of TWPs, and the effective concentrations after the pre-incubation were much lower than environmentally relevant concentrations. The lifetime of C. elegans was reduced faster in the TWP treatment groups, and the effective concentration for lifetime exposure tests were 100- to 1,000-fold lower than those of short-term exposure tests. Water-extractable metal concentrations (Cr, Cu, Ni, Pb, and Zn) in the TWP-soils showed no correlation with nominal TWP concentrations or pre-incubation times, and the incorporated metals in the TWPs may be not the main reason of toxicity in this study. Our results show that toxic effects of TWPs can be time-dependent, both in terms of the microplastic particles themselves and their interactions in the soil matrix, but also because of susceptibility of target organisms depending on developmental stage. It is vital that future work consider these aspects, since otherwise effects of microplastics and TWPs could be underestimated.Graphical Abstract