bluuebunny/crossref_metadata_2025_split huggingface dataset:
Aerobic Training Alleviates Muscle Atrophy by Promoting the
Proliferation of Skeletal Muscle Satellite Cells in Myotonic Dystrophy
Type 1 by Inhibiting Glycolysis via the Upregulation of MBNL1
Authors: Wang Hui-Qi , Guo Min , Lu Jie-Qiong , Chen Ling-Yun , Liang Feng , Huang Peng-Peng , Song Kai-Yi 
Published: December 2024

Background:
Skeletal muscle atrophy in myotonic dystrophy type 1 (DM1) is caused
by abnormal skeletal muscle satellite cell (SSC) proliferation due to increased glycolysis, which
impairs muscle regeneration. In DM1, RNA foci sequester muscleblind-like protein 1 (MBNL1)
in the nucleus, inhibiting its role in regulating SSC proliferation. Aerobic training reduces glycolysis
and increases SSC proliferation and muscle fiber volume. This study aimed to investigate
whether aerobic training prevents muscle atrophy in DM1 through the regulation of glycolysis via
MBNL1.

Methods:
In this study, we used the HSALR transgenic mice (DM1 mice model) to investigate the
effects of aerobic training on skeletal muscle atrophy and its molecular mechanisms. HSALR mice
were subjected to 4 weeks of aerobic training. After aerobic training, hindlimb grip, and myofiber
mean cross-sectional area (CSA) detected by haematoxylin and eosin (HE) staining were performed.
In DM1 primary SSCs, cell proliferation was assessed using Pax7 and MyoD immunofluorescence
and CCK-8 assays, RNA foci were detected by RNA fluorescence in situ hybridization,
and total MBNL1 expression was measured by western blot. We also used lentivirus to knock
down MBNL1 in DM1 primary SSCs and performed RNA sequencing and extracellular acidification
rate (ECAR). Furthermore, glycolysis detected by ECAR and oxygen consumption rate
(OCR) assays were performed in WT, Sedentary, and Training group SSCs. Glycolysis was inhibited
with shikonin, a glycolysis inhibitor, and the proliferation of DM1 SSCs was subsequently
evaluated. Finally, we engineered an adeno-associated virus specifically targeting MBNL1 to
knock down MBNL1 in DM1 mice. Subsequently, we assessed hindlimb grip strength and CSA
in vivo, as well as the glycolytic capacity and proliferative capacity of DM1 SSCs in vitro.

Results:
Aerobic training increased hindlimb grip strength and the average myofiber CSA in
DM1 mice. Additionally, aerobic training reduced RNA foci, upregulated MBNL1, and promoted
SSC proliferation. Gene set enrichment analysis (GSEA) indicated that glycolytic processes were
enriched following the knockdown of MBNL1. Furthermore, ECAR showed glycolysis was enhanced
after the knockdown of MBNL1. Aerobic training reduced elevated glycolysis in DM1
mice and primary SSCs. Treatment with shikonin promoted DM1 SSC proliferation. However,
MBNL1 knockdown was shown to abolish the reduced glycolysis and increased proliferation capability
of SSCs due to aerobic training.

Conclusion:
Taken together, aerobic training suppresses glycolysis in SSCs via the upregulation
of MBNL1, thereby enhancing SSC proliferation and alleviating muscle atrophy.
The effect of comorbid conditions on the duration of pain syndrome in patients with total hip replacement and the ability to manage them
Authors: Tikhomirova N. Yu. , Zhikhareva O. A. , Eliseyeva L. N. , Zhdamarova O. I. , Bondarenko M. N. 
Published: December 2024

Objective: identification of risk factors for long-term pain syndrome in patients with total hip replacement (TEP) and the possibility of managing them.Material and methods: 122 patients were examined. Inclusion criteria: men and women aged 55-67 years, suffering from stage III osteoarthritis of the hip joint (OA TBS), compensated type 2 diabetes mellitus (DM), who signed an informed consent for dynamic follow-up. All patients were divided into 2 groups: group 1 (n=62) had a combination of OA TBS+DM, group 2 (n=60) included patients with OA TBS without DM. All the examined patients underwent an assessment of concomitant pathology, an anthropometric study with the calculation of body mass index, a study of morphometric parameters of the heart by echocardiography, as well as the calculation of glomerular filtration rate using the CKD-EPI formula. The presence of pain syndrome was assessed for more than 3 months after surgery. Statistical processing of the obtained data was performed using the package of licensed statistical programs STATISTICA 6.0 (StatSoftInc., USA).Results: in our study, the risk of pain syndrome increased for more than 3 months: age, concomitant pathology (diabetes mellitus, hypertension, coronary heart disease, varicose veins of the lower extremities with thrombosis), duration of osteoarthritis of the hip joint, decreased GFR. In our work, the proportion of people with GFR <60 ml/min/1.73 m2 was significantly higher among patients with OA TBS+DM, as well as with a duration of OA TBS for more than 10 years and the presence of DM, which can be explained by the negative effect on the kidneys of taking painkillers with long-term OA, as well as the influence of high levels blood glucose (against the background of concomitant diabetes) on the glomerular apparatus. Among patients with OA TBS+DM without SYSADOA, the proportion of people with GFR<60 ml/min/1.73 m2 was 3.1 times higher than among people with OA TBS without DM+ SYSADOA (p<0.05).Conclusions: monitoring the intake of nonsteroidal anti-inflammatory drugs in the preoperative period, correction of concomitant pathology, as well as compliance with national recommendations for the treatment of coxarthrosis in terms of taking SYSADOA and physical exercises will reduce the risk of prolonged pain syndrome in the postoperative period of hip TEP.
Machine Learning and Deep Learning for Diagnosis of Lumbar Spinal Stenosis: Systematic Review and Meta-Analysis
Authors: Wang Tianyi , Chen Ruiyuan , Fan Ning , Zang Lei , Yuan Shuo , Du Peng , Wu Qichao , Wang Aobo , Li Jian , Kong Xiaochuan , Zhu Wenyi 
Published: December 2024

Background
Lumbar spinal stenosis (LSS) is a major cause of pain and disability in older individuals worldwide. Although increasing studies of traditional machine learning (TML) and deep learning (DL) were conducted in the field of diagnosing LSS and gained prominent results, the performance of these models has not been analyzed systematically.

Objective
This systematic review and meta-analysis aimed to pool the results and evaluate the heterogeneity of the current studies in using TML or DL models to diagnose LSS, thereby providing more comprehensive information for further clinical application.

Methods
This review was performed under the PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-Analyses) guidelines using articles extracted from PubMed, Embase databases, and Cochrane Library databases. Studies that evaluated DL or TML algorithms assessment value on diagnosing LSS were included, while those with duplicated or unavailable data were excluded. Quality Assessment of Diagnostic Accuracy Studies 2 was used to estimate the risk of bias in each study. The MIDAS module and the METAPROP module of Stata (StataCorp) were used for data synthesis and statistical analyses.

Results
A total of 12 studies with 15,044 patients reported the assessment value of TML or DL models for diagnosing LSS. The risk of bias assessment yielded 4 studies with high risk of bias, 3 with unclear risk of bias, and 5 with completely low risk of bias. The pooled sensitivity and specificity were 0.84 (95% CI: 0.82-0.86; I2=99.06%) and 0.87 (95% CI 0.84-0.90; I2=98.7%), respectively. The diagnostic odds ratio was 36 (95% CI 26-49), the positive likelihood ratio (LR+) was 6.6 (95% CI 5.1-8.4), and the negative likelihood ratio (LR–) was 0.18 (95% CI 0.16-0.21). The summary receiver operating characteristic curves, the area under the curve of TML or DL models for diagnosing LSS of 0.92 (95% CI 0.89-0.94), indicating a high diagnostic value.

Conclusions
This systematic review and meta-analysis emphasize that despite the generally satisfactory diagnostic performance of artificial intelligence systems in the experimental stage for the diagnosis of LSS, none of them is reliable and practical enough to apply in real clinical practice. Further efforts, including optimization of model balance, widely accepted objective reference standards, multimodal strategy, large dataset for training and testing, external validation, and sufficient and scientific report, should be made to bridge the distance between current TML or DL models and real-life clinical applications in future studies.

Trial Registration
PROSPERO CRD42024566535; https://tinyurl.com/msx59x8k
Evaluating Bard Gemini Pro and GPT-4 Vision Against Student Performance in Medical Visual Question Answering: Comparative Case Study
Authors: Roos Jonas , Martin Ron , Kaczmarczyk Robert 
Published: December 2024

Abstract

Background
The rapid development of large language models (LLMs) such as OpenAI’s ChatGPT has significantly impacted medical research and education. These models have shown potential in fields ranging from radiological imaging interpretation to medical licensing examination assistance. Recently, LLMs have been enhanced with image recognition capabilities.

Objective
This study aims to critically examine the effectiveness of these LLMs in medical diagnostics and training by assessing their accuracy and utility in answering image-based questions from medical licensing examinations.

Methods
This study analyzed 1070 image-based multiple-choice questions from the AMBOSS learning platform, divided into 605 in English and 465 in German. Customized prompts in both languages directed the models to interpret medical images and provide the most likely diagnosis. Student performance data were obtained from AMBOSS, including metrics such as the “student passed mean” and “majority vote.” Statistical analysis was conducted using Python (Python Software Foundation), with key libraries for data manipulation and visualization.

Results
GPT-4 1106 Vision Preview (OpenAI) outperformed Bard Gemini Pro (Google), correctly answering 56.9% (609/1070) of questions compared to Bard’s 44.6% (477/1070), a statistically significant difference (χ2₁=32.1, P<.001). However, GPT-4 1106 left 16.1% (172/1070) of questions unanswered, significantly higher than Bard’s 4.1% (44/1070; χ2₁=83.1, P<.001). When considering only answered questions, GPT-4 1106’s accuracy increased to 67.8% (609/898), surpassing both Bard (477/1026, 46.5%; χ2₁=87.7, P<.001) and the student passed mean of (674/1070, SE 1.48%; χ2₁=4.8, P=.03). Language-specific analysis revealed both models performed better in German than English, with GPT-4 1106 showing greater accuracy in German (282/465, 60.65% vs 327/605, 54.1%; χ2₁=4.4, P=.04) and Bard Gemini Pro exhibiting a similar trend (255/465, 54.8% vs 222/605, 36.7%; χ2₁=34.3, P<.001). The student majority vote achieved an overall accuracy of 94.5% (1011/1070), significantly outperforming both artificial intelligence models (GPT-4 1106: χ2₁=408.5, P<.001; Bard Gemini Pro: χ2₁=626.6, P<.001).

Conclusions
Our study shows that GPT-4 1106 Vision Preview and Bard Gemini Pro have potential in medical visual question-answering tasks and to serve as a support for students. However, their performance varies depending on the language used, with a preference for German. They also have limitations in responding to non-English content. The accuracy rates, particularly when compared to student responses, highlight the potential of these models in medical education, yet the need for further optimization and understanding of their limitations in diverse linguistic contexts remains critical.
An improved Fuzzy multiple object clustering in remodeling of roofs with perceptron algorithm
Authors: D. Neguja  , A. Senthilrajan  
Published: December 2024

The novel way is completely discrete technique to remodel the roof of old buildings from the real value related repetition. Pointed quantities of partition for most of the developing renovation or modifications at the roof via a strategy are some parameters simplifying a fuzzy multiple object technique, where every segment is linked to all clusters with specialized matching weights of roof segments. The strategy considers multiple objects of perceptron algorithm across the indepth joints and incorporates of 3 layers: (i) every part is featued with the leading direction of a vector of exact measures of the roof densities, (ii) most required factor of evaluation is completed to review the principle changes in lowing the outcomes of the clatter, and (iii) the squared Euclidean location most of the number one retained major components is used to c-arry out clustering through the equal vintage fuzzy Multiple object-approach using perceptron method. A perceptron technique, multiple object is applied strategy in this research and the image parts and its neighboring segments are added to form a cluster by using the use of immediate computation of the resulting fuzzy number and overall idea of the process of the technique is to compute the mapping concept of sequentially located from equally well-defined clusters. The fuzzy number is applied to multiple objects using perceptron algorithm of the segment and compared with fuzzy technique. The outcome is to bring the nearest neighbor of the fuzzy value,.
Studies on the Effect of Bagging on Yield and Quality of Mango cv. Himsagar
Authors: Ramez Noorhabeeb , Subba Susan , Taheri Mohammad Naser , Alam Mahabub , Hasan Md Abu 
Published: December 2024

The present experiment was carried out twice in a mango orchard situated in the village of Ghoragacha, Nadia, West Bengal (741 245), India during the months of March to June in the year 2019 and 2020 with twenty-one trees of mango cultivar himsagar comprising seven treatments viz. T1: Non-woven yellow bag, T2: Non-woven red bag, T3: Non-woven Blue bag, T4: Non-woven white bag, T5: Non-woven green bag, T6: Newspaper and T7: Control was laid out in randomized block design with three replication. The result from the present experiment revealed that there were significant variation in fruit weight, fruit length, fruit diameter, fruit volume, fruit peel weight and fruit seed weight. Healthy bearer and productive mango trees were selected and the fruits when at pea stage were considered for bagging. After bagging in a different coloured paper bags and newspaper bags, stapled is done carefully to cover the fruits from the direct sunlight and also to avoid open space for entry of insects or rain, etc. The physical and chemical data were collected from both the year i.e., 2019 and 2020 and an average data were given. The highest fruit weight (256.00 g), highest fruit volume (224.38 ml), highest fruit length (89.68 mm), highest fruit diameter (57.15 cm) and highest fruit peel weight were observed in T5 treatment, where non-woven green bags were used and highest fruit seed weight (57.15 cm) was observed in control. However, there were no such significant changes in terms of chemical properties of fruits.
Evaluation of Seed Germination of Chironji (Buchanania cochinchinensis (Lour.) M. R. Almedia) under Nursery Conditions
Authors: Malakar Ayushman , Kumar Rikesh , Mishra Yogeshwar , Sinha Animesh 
Published: December 2024

The study was carried out during the month of June to August in the year, 2022 in the FGR field in the nursery of Institute of Forest Productivity (IFP), Lalgutwa, Ranchi, Jharkhand (835303), India to find out effective germination techniques to expedite nursery propagation of Chironji, an economically significant non-timber forest produce (NTFP) in red and lateritic zone (RLZ) of eastern India. The research evaluated seven pre-treatments to enhance germination potential using seeds harvested from a Candidate Plus Tree (CPT) in the natural forest of Burdwan Forest Division, West Bengal. The study employed a completely randomized design with four replications, evaluating various germination parameters over a 50-day period. The combination of hot water treatment and mechanical scarification (T6) exhibited highest germination of 87.5% and also outperformed all other pre-treatments in every germination parameter evaluated. It also yielded the highest seed vigour indices (SVI) of 1303.53 (SVI-I) and 109.155 (SVI-II). This method proved to be the most effective for breaking seed dormancy and promoting robust seedling development of Chironji for the prevailing agroclimatic conditions in RLZ of eastern India. The outcomes of this study demonstrate an affordable and swift seed treatment method without relying on costly growth hormones or chemicals contributing to conservation efforts. Given the challenges of seed dormancy in this species, marginal farmers and state forest departments (SFDs) can employ the pre-treatment reported in the study for commercial cultivation of Chironji as well as contributing to conservation efforts of the species.
Daya Simpan Benih Botani Bawang Merah (Allium cepa var. agregatum) Setelah Invigorasi
Authors: Adhinugraha Qudus Sabha , Widajati Eny , Palupi Endah Retno 
Published: December 2024

Benih botani bawang merah (TSS) merupakan alternatif bahan tanam dalam budidaya bawang merah. Penggunaan TSS sebagai bahan tanam memiliki keunggulaan dibandingkan dengan benih asal umbi, antara lain kebutuhan benih yang lebih sedikit, dapat memutus siklus patogen, tidak ada masa dormansi, dan dapat disimpan dalam jangka waktu lebih dari satu tahun. Walaupun dapat disimpan dalam jangka waktu yang lama, namun TSS tetap akan mengalami deteriorasi atau kemunduran yang dapat menyebabkan penuruan mutu benih. Salah satu upaya yang dapat dilakukan untuk meningkatkan kembali mutu benih adalah dengan perlakuan invigorasi KNO3. Saat ini peran invigorasi terhadap daya simpan benih TSS masih belum diketahui. Penelitian ini dilaksanakan Laboratorium Penyimpanan dan Pengujian Mutu Benih IPB University, Indonesia. Penelitian ini bertujuan untuk mengetahui informasi daya simpan TSS setelah invigorasi dan pengeringan. Percobaan disusun menggunakan rancangan tersarang (nested design) dua faktor. Faktor pertama adalah kondisi ruang simpan benih yang terdiri atas tiga taraf, yaitu suhu 28±2˚C, RH 73±7% (kondisi kamar); suhu 18±2 ˚C, RH 61±7% (kondisi ruang ber-AC); dan suhu 8±2˚C, RH 24±8% (kondisi lemari es). Faktor kedua adalah jenis kemasan, yang terdiri atas dua jenis, yaitu kemasan alumunium foil (ketebalan 0,08 mm) dan kemasan plastik polipropilen (PP) (ketebalan 0,06 mm). Faktor jenis kemasan tersarang pada faktor kondisi ruang simpan benih. Hasil penelitian menunjukkan bahwa Mutu TSS pasca invigorasi dapat dipertahankan selama 16 MSS dalam ruang ber-AC (suhu 18±2 ˚C, RH 61±7%) dengan kemasan alumunium foil atau lemari es (suhu 8±2˚C, RH 24±8%) dengan kemasan alumunium foil atau plastik PP.
PENGARUH KANDUNGAN HARA TANAH DAN KLON TERHADAP KADAR PATI UBI KAYU (Manihot esculenta Crantz) DI LAMPUNG TENGAH YANG DIUKUR DENGAN METODE NERACA MASSA
Authors: Yelli Fitri , Wahyudi Muhammad , Surtono Arif , Supriatin Supriatin 
Published: December 2024

Kadar pati sangat mempengaruhi jumlah produksi tepung tapioka yang dihasilkan. Kandungan pati pada ubi kayu dipengaruhi oleh klon, umur panen optimal umbi, kondisi cuaca, serta faktor unsur hara tanah selama periode pertumbuhan hingga panen Apabila unsur hara pada lahan berkurang maka akan mempengaruhi hasil kadar pati pada tanaman ubi kayu, sehingga terjadi degradasi komponen pati. Tujuan penelitian ini adalah untuk mengetahui pengaruh kandungan hara tanah terhadap kadar pati ubi kayu dan mengetahui pengaruh klon ubi kayu yang dibudidayakan pada kondisi tanah yang berbeda terhadap kadar pati ubi kayu. Penelitian ini dilaksanakan di Laboratorium Ilmu Tanah, Fakultas Pertanian, Universitas Lampung. Dilaksanakan pada bulan Oktober 2023 hingga Januari 2024. Penelitian dilakukan dengan perlakuan 4 jenis klon ubi kayu yaitu klon Klantang, Daun Sembilan, Kasetsart dan Garuda. Setiap klon diambil sebanyak 4 titik sampel ubi kayu dengan umur panen 8 bulan dan 4 titik sampel tanah di Desa Tanjung Anom, Kecamatan Terusan Nunyai, Kabupaten Lampung Tengah. Sampel ubi kayu diambil untuk diukur kadar pati dengan metode neraca massa, sedangkan sampel tanah diambil untuk dianalisis pH tanah, nitrogen, fosfor, kalium dan C organik tanah. Penelitian ini menggunakan uji analisis deskriptif dengan standar deviasi untuk mendeskripsikan hasil pengukuran kadar pati ubi kayu. Sedangkan uji korelasi dilakukan untuk mengetahui pengaruh pH dan unsur hara tanah terhadap kadar pati ubi kayu. Hasil korelasi menunjukkan nitrogen berpengaruh terhadap kadar pati ubi kayu. Berdasarkan analisis deskriptif dengan standar deviasi kadar pati tertinggi terdapat pada klon Kasetsart dan Garuda, sedangkan kadar pati terendah terdapat pada klon Klantang dan Daun Sembilan.
“Felicidades hermanas”. Sociabilidad y subjetividad entre seguidoras argentinas de Taylor Swift
Authors: Gonzalez Julieta , Rodriguez Rocío María 
Published: December 2024

El presente artículo analiza la construcción del gusto por Taylor Swift mediante el acercamiento etnográfico a las prácticas de sus fans en el contexto argentino. Para ello, el texto aborda escenarios del mundo swiftie online y offline, que tenían especial relevancia para la producción de sentidos de comunidad y formas de hacer lo social, pero también de interpretar y realizar lo individual. Específicamente, indaga la producción de sentidos presente en trendig topics, memes y trends que circulaban por redes sociales durante la época en la que tuvo lugar el primer paso de la cantante por escenarios argentinos. Ese análisis permite reflexionar sobre los complejos entramados en los que se entretejían los mercados, las músicas y las prácticas aficionadas. Particularmente el caso swiftie, permitió identificar formas de construcción subjetiva y colectiva que ocurrían de manera dialógica entre el mundo online y los espacios offline, territorios donde se disputaban y realizaban performativamente formas de ser y de hacer.
A generic precurved interlocking nail is more compatible with femoral and tibial morphology than a straight nail in dogs
Authors: Rousseau Thomas , Jourdain Mathieu , Godart Benjamin , Maitre Pierre , Cachon Thibaut , Leperlier Dimitri , Gauthier Olivier 
Published: December 2024

Abstract

OBJECTIVE
To study the morphology of canine and feline femurs and tibias in lateral radiographic projections and assess their compatibility with either a straight or a curved full-length interlocking nail (ILN).

METHODS
Lateral projection radiographs of 50 tibias and 50 femurs (10 cats and 40 dogs per bone) were used to measure the minimum and maximum radius of curvature of an ILN compatible with each bone. These radii were defined by cranial and caudal endosteal points at the proximal entry point of the nail, at the isthmus, and at the most distal point of the ILN insertion into the femoral or tibial metaphysis. These points were figured by 2 proximal circles, 2 diaphyseal circles at the isthmus, and 2 distal circles in distal metaphyses of the bones. The diameter of these circles corresponded to 75% of the medullary canal width at the isthmus of the bone.

RESULTS
An ILN with a radius between 750 and 806 mm fit 87% of all the bones reviewed. In dogs, an ILN within this radius range would fit 95% of the tibias, whereas a straight ILN would fit only 50%. For femurs, the curved ILN would fit 80% compared to 37.5% for a straight ILN. In cats, an ILN with a radius between 750 and 806 mm would fit 85% of the bones (femur, 100%; tibia, 70%), whereas a straight ILN would fit all tested bones.

CONCLUSIONS
A full-length ILN with a radius of curvature between 750 and 806 mm would fit 87% of the studied bones, whereas only 55% of them could accept a straight ILN.

CLINICAL RELEVANCE
A full-length ILN with a 750–806-mm curvature radius may provide a suitable option for treating most femoral and tibial diaphyseal fractures.