bluuebunny/crossref_metadata_2025_split huggingface dataset:
Is Human Chorionic Gonadotropin Trigger Beneficial for Natural Cycle Frozen-Thawed Embryo Transfer?
Authors: Gao Dan-Dan , Li Li , Zhang Yi , Wang Xiao-Xuan , Song Jing-Yan , Sun Zhen-Gao 
Published: October 2021

Objective: The aim of this study is to investigate, in ovulatory patients, whether there is a difference in reproductive outcomes following frozen-thawed embryo transfer (FET) in natural cycles (NC) compared to modified natural cycles (mNC).Methods: This retrospective cohort study, performed at the public tertiary fertility clinic, involved all infertile patients undergoing endometrial preparation prior to FET in NC and mNC from January, 2017 to November, 2020. One thousand hundred and sixty-two patients were divided into two groups: mNC group (n = 248) had FET in a NC after ovulation triggering with human chorionic gonadotropin (hCG); NC group (n = 914) had FET in a NC after spontaneous ovulation were observed. The primary outcome was live birth rate. All pregnancy outcomes were analyzed by propensity score matching (PSM) and multivariable logistic regression analyses.Results: The NC group showed a higher live birth rate [344/914 (37.6%) vs. 68/248 (27.4%), P = 0.003; 87/240 (36.3%) vs. 66/240 (27.5%), P = 0.040] than the mNC group before and after PSM analysis. Multivariable analysis also showed mNC to be associated with a decreased likelihood of live birth compared with NC [odds ratio (OR) 95% confidence interval (CI) 0.71 (0.51–0.98), P = 0.039].Conclusion: For women with regular menstrual cycles, NC-FET may have a higher chance of live birth than that in the mNC-FET cycles. As a consequence, it's critical to avoid hCG triggering as much as possible when FETs utilize a natural cycle strategy for endometrial preparation. Nevertheless, further more well-designed randomized clinical trials are still needed to determine this finding.
Lactitol Supplementation Modulates Intestinal Microbiome in Liver Cirrhotic Patients
Authors: Lu Haifeng , Chen Liang , Pan Xiaxia , Yao Yujun , Zhang Hua , Zhu Xiaofei , Lou Xiaobin , Zhu Chunxia , Wang Jun , Li Lanjuan , Wu Zhongwen 
Published: October 2021

Background: Cirrhosis is a common chronic liver disease characterized by irreversible diffuse liver damage. Intestinal microbiome dysbiosis and metabolite dysfunction contribute to the development of cirrhosis. Lactitol (4-β-D-galactopyranosyl-D-glucitol) was previously reported to promote the growth of intestinal Bifidobacteria. However, the effect of lactitol on the intestinal microbiome and fecal short-chain fatty acids (SCFAs) and bile acids (BAs) and the interactions among these factors in cirrhotic patients pre- and post-lactitol treatment remain poorly understood.Methods: Here, using shotgun metagenomics and targeted metabolomics methods.Results: we found that health-promoting lactic acid bacteria, including Bifidobacterium longum, B.pseudocatenulatum, and Lactobacillus salivarius, were increased after lactitol intervention, and significant decrease of pathogen Klebsiella pneumonia and associated antibiotic resistant genes /virulence factors. Functionally, pathways including Pseudomonas aeruginosa biofilm formation, endotoxin biosynthesis, and horizontal transfer of pathogenic genes were decreased in cirrhotic patients after 4-week lactitol intervention compared with before treatment.Conclusion: We identified lactitol-associated metagenomic changes, and provide insight into the understanding of the roles of lactitol in modulating gut microbiome in cirrhotic patients.
SYDE1 Acts as an Oncogene in Glioma and has Diagnostic and Prognostic Values
Authors: Han Zhenyuan , Zhuang Xiaodong , Yang Biao , Jin Lihui , Hong Pengjie , Xue Junqing , Chen Shunjie , Tian Zhen 
Published: October 2021

Objectives: Gliomas remain one of serious public health problems worldwide which demand further and deeper investigation. The aim of this study was to explore the association between synapse defective protein 1 homolog 1 (SYDE1) and gliomas via public database analysis and in vitro validation to determine the potential diagnostic and prognostic values.Methods and Results: Compared with healthy brain tissues, there was a significant increase in SYDE1 expression in glioma tissues. Additionally, SYDE1 exhibited higher expression levels in glioma patients with unfavorable clinicopathological factors. In vitro knockdown of SYDE1 in glioma cell lines A172 inhibited their migrative and invasive ability but not the proliferative ability. GO and KEGG pathway analysis of the top 100 genes coexpressed with SYDE1 showed enrichments of tumor-associated terms. Further bioinformatic analysis revealed that the SNHG16/hsa-miR-520e/SYDE1 axis might be involved in glioma development.Conclusions:SYDE1 is expressed at higher levels in gliomas than in healthy brains, and can promote metastasis and invasion but not proliferation of gliomas. Furthermore, SYDE1 has values in the diagnosis and prognosis prediction of gliomas.
A Bibliometric Analysis of 34,692 Publications on Thyroid Cancer by Machine Learning: How Much Has Been Done in the Past Three Decades?
Authors: Zhang Zeyu , Yao Lei , Wang Wenlong , Jiang Bo , Xia Fada , Li Xinying 
Published: October 2021

IntroductionThyroid cancer (TC) is the most common neck malignancy. However, a large number of publications of TC have not been well summarized and discussed with more comprehensive methods. The purpose of this bibliometric study is to summarize scientific publications during the past three decades in the field of TC using a machine learning method.Material and MethodsScientific publications focusing on TC from 1990 to 2020 were searched in PubMed using the MeSH term “thyroid neoplasms”. Full associated data were downloaded in the format of PubMed, and extracted in the R platform. Latent Dirichlet allocation (LDA) was adopted to identify the research topics from the abstract of each publication using Python.ResultsA total of 34,692 publications related to TC from the last three decades were found and included in this study with an average of 1,119.1 publications per year. Clinical studies and experimental studies shared the most proportion of publications, while the proportion of clinical trials remained at a relatively small level (5.87% as the highest in 2004). Thyroidectomy was the lead MeSH term, followed by prognosis, differential diagnosis, and fine-needle biopsy. The LDA analyses showed the study topics were divided into four clusters, including treatment management, basic research, diagnosis research, epidemiology, and cancer risk. However, a relatively weak connection was shown between treatment managements and basic researches. Top 10 most cited publications in recent years particularly highlighted the applications of active surveillance in TC.ConclusionThyroidectomy, differential diagnosis, genomic analysis, active surveillance are the most concerning topics in TC researches. Although the BRAF-targeted therapy is under development with promising results, there is still an urgent need for conversions from basic studies to clinical practice.
The Influence of Different Treatment Strategies on the Long-Term Prognosis of T1 Stage Esophageal Cancer Patients
Authors: Pan Liang , Liu Xingyu , Wang Weidong , Zhu Linhai , Yu Wenfeng , Lv Wang , Hu Jian 
Published: October 2021

ObjectiveTo compare the long-term prognosis effects of non-esophagectomy and esophagectomy on patients with T1 stage esophageal cancer.MethodsAll esophageal cancer patients in the study were included from the National Surveillance Epidemiology and End Results (SEER) database between 2005-2015. These patients were classified into non-esophagectomy group and esophagectomy group according to therapy methods and were compared in terms of esophagus cancer specific survival (ECSS) and overall survival (OS) rates.ResultsA total of 591 patients with T1 stage esophageal cancer were enrolled in this study, including 212 non-esophagectomy patients and 111 esophagectomy patients in the T1a subgroup and 37 non-esophagectomy patients and 140 esophagectomy patients in the T1b subgroup. In all T1 stage esophageal cancer patients, there was no difference in the effect of non-esophagectomy and esophagectomy on postoperative OS, but postoperative ECSS in patients treated with non-esophagectomy was significantly better than those treated with esophagectomy. Cox proportional hazards regression model analysis showed that the risk factors affecting ECSS included race, primary site, tumor size, grade, and AJCC stage but factors affecting OS only include tumor size, grade, and AJCC stage in T1 stage patients. In the subgroup analysis, there was no difference in either ECSS or OS between the non-esophagectomy group and the esophagectomy group in T1a patients. However, in T1b patients, the OS after esophagectomy was considerably better than that of non-esophagectomy.ConclusionsNon-esophagectomy, including a variety of non-invasive procedures, is a safe and available option for patients with T1a stage esophageal cancer. For some T1b esophageal cancer patients, esophagectomy cannot be replaced at present due to its diagnostic and therapeutic effect on lymph node metastasis.
GSTT1null and rs156697 Polymorphism in GSTO2 Influence the Risk and Therapeutic Outcome of B-Acute Lymphoblastic Leukemia Patients
Authors: Baba Shahid M. , Pandith Arshad A. , Shah Zafar A. , Geelani Sajad A. , Bhat Javid R. , Gul Ayaz , Guru Sameer A. , El-Serehy Hamed A. , Koul Abid M. , Mansoor Sheikh 
Published: October 2021

IntroductionGlutathione S-transferase (GST) gene deletion or polymorphic sequence variations lead to decreased enzyme activity that influences susceptibility and response to chemotherapy in acute lymphoblastic leukemia (ALL). This case–control study investigated the association of GST gene polymorphisms with the etiology and therapeutic outcome of B-ALL among Kashmiri population.MethodsA total of 300 individuals including 150 newly diagnosed B-ALL patients and an equal number of age and gender matched controls were genotyped for five GST gene polymorphisms by polymerase chain reaction–restriction fragment length polymorphism technique (PCR-RFLP) and multiplex PCR techniques.ResultsHigher frequency of GSTT1null, GSTO2-AG, and GSTO2-GG genotypes was observed in ALL cases compared to controls that associated significantly with ALL risk (GSTT1null: OR = 2.93, p = 0.0001; GSTO2-AG: OR = 2.58, p = 0.01; GSTO2-GG: OR = 3.13, p = 0.01). GSTM1, GSTP1, and GSTO1 SNPs showed no significant association (p &gt; 0.05). Combined genotype analysis revealed significant association of GSTT1null/GSTM1null (OR = 4.11, p = 0.011) and GSTT1null/GSTP1-AG (OR = 4.93, p = 0.0003) with B-ALL susceptibility. Haplotype analysis of rs4925 and rs156697 revealed that carriers of CG haplotype had increased risk of B-ALL (p = 0.04). Kaplan–Meier plots revealed significantly inferior 3-year disease-free survival for GSTO2-GG carriers (p = 0.002). Multivariate analysis confirmed GSTO2-GG as an independent poor prognostic factor for DFS (HR = 4.5, p = 0.034). Among combined genotypes, only GSTT1null/GSTP1-AG associated significantly with poorer DFS rates (p = 0.032).ConclusionThis study demonstrated that GSTT1null individually or in combination with GSTM1null and GSTP1-AG genotypes associated with increased B-ALL risk. Also, rs156697 variant genotypes (AG and GG) associated with B-ALL, whereas the GG genotype of rs156697 influenced the treatment outcome.
Mechanisms Underlying the Effects of Lianhua Qingwen on Sepsis-Induced Acute Lung Injury: A Network Pharmacology Approach
Authors: Yang Ruhao , Yang Haizhen , Wei Jie , Li Wenqiang , Yue Fang , Song Yan , He Xin , Hu Ke 
Published: October 2021

Background and Purpose: Sepsis is a life-threatening condition associated with secondary multiple organ injury. Acute lung injury (ALI) caused by sepsis has high morbidity and mortality in critical care units. Lianhua Qingwen (LHQW) is a traditional Chinese medicine composing of 11 herbs and 2 medicinal minerals. LHQW exhibits anti-inflammatory activity and is effective in treating pneumonia. Our study aimed to evaluate the effect of LHQW on sepsis-induced ALI and its underlying mechanism.Materials and Methods: A network pharmacology approach was used to predict the bioactive components and effective targets of LHQW in treating ALI. We established ALI model C57/BL6 mice via an intraperitoneal injection of LPS and inhibited p53 expression by pifithrin-α, in order to validate the mechanism by which LHQW exerted protective role in ALI. Hematoxylin-eosin staining was conducted to assess the severity of lung injury. The severity of inflammation was evaluated based on MPO (myeloperoxidase) activity. TUNEL assay was employed to detect apoptotic cells. The levels of p53 and caspase-3 were tested by immunohistochemical staining and Western blotting. The expression levels of Bcl-2, Bax, cytochrome C and caspase-9 were detected by Western blotting.Results: A total of 80 genes were associated with LHQW in the treatment of ALI. After PPI network construction, four active components (quercetin, luteolin, kaempferol and wogonin) and 10 target genes (AKT1, TP53, IL6, VEGFA, TNF, JUN, STAT3, MAPK8, MAPK1, and EGF) were found to be essential for ALI treatment. GO and KEGG analyses indicated that apoptosis pathway was mainly involved in the LHQW-ALI network. Animal experiments showed that LHQW was able to attenuate LPS-induced ALI, and medium-dose LHQW exhibited the most prominent effect. LHQW could inhibit the overexpression of p53 induced by LPS and suppress p53-mediated intrinsic apoptotic pathways by decreasing the levels of Bax, caspase-3 and caspase-9, increasing the expression of Bcl-2, and attenuating the release of cytochrome C in ALI mice.Conclusion: This study reveals that LHQW may alleviate LPS-induced ALI via inhibiting p53-mediated intrinsic apoptosis pathways.
Efficacy of a Simplified Feedback Trainer for High-Quality Chest Compression Training: A Randomized Controlled Simulation Study
Authors: Meng Xiao-yan , You Jia , Dai Li-li , Yin Xiao-dong , Xu Jian-an , Wang Jia-feng 
Published: October 2021

Background: The most recent international guidelines recommended support training of chest compression (CC) using feedback devices. This study aimed to compare the training efficacy of a simplified feedback trainer with the traditional cardiopulmonary resuscitation (CPR) simulator in CPR training.Methods: A total of 60 soldiers were randomly allocated into three groups equally, trained with a simplified external cardiac massage (ECM) trainer named Soul SheathTM (SS) (SS group), a Resusci Anne manikin (RA group), or traditional simulation training with instructor feedback, respectively. After 7 days of training, the CPR skills were tested blindly in a 2-min assessment session. The primary outcome was the proportion of effective CC, and the secondary outcome included CC rate, depth, compression position, and extent of the release.Results: The percentage of effective CC achieved in the SS group was comparable with the RA group (77.0 ± 15.52 vs. 77.5 ± 10.73%, p = 0.922), and significantly higher than that in the control group (77.0 ± 15.52 vs. 66.8 ± 16.87%, p = 0.037). Both the SS and RA groups showed better CC performance than the control group in terms of CC rate (SS group vs. control group, P = 0.032 and RA group vs. control group, P = 0.026), the proportion of shallow CC (SS group vs. control group, P = 0.011 and RA group vs. control group, P = 0.017). No difference between the SS group and RA group was found in all the CC parameters.Conclusions: The simplified ECM trainer (SS) provides a similar efficacy to the traditional manikin simulator with feedback in CC training to improve the quality of CPR skills.
Identification of DNA Bases and Their Cations in Astrochemical Environments: Computational Spectroscopy of Thymine as a Test Case
Authors: Zhao Yage , Hochlaf Majdi , Biczysko Malgorzata 
Published: October 2021

Increased importance of vibrational fingerprints in the identification of molecular systems, can be highlighted by the upcoming interstellar medium (ISM) observations by the James Webb Space Telescope, or in a context of other astrochemical environments as meteorites or exoplanets, Mars robotic missions, such as instruments on board of Perseverance rover. These observations can be supported by combination of laboratory experiments and theoretical calculations, essential to verify and predict the spectral assignments. Astrochemical laboratory simulations have shown that complex organic molecules (COMs) can be formed from simple species by vacuum ultraviolet or X-ray irradiation expanding interest in searching for organic biological and prebiotic compounds. In this work an example of nucleobase, thymine, is selected as a test case for highlighting the utility of computational spectroscopic methods in astrochemical studies. We consider mid-infrared (MIR) and near-infrared (NIR) vibrational spectra of neutral (T) and cationic (T+) thymine ground states, and vibrationally-resolved photoelectron (PE) spectra in the far UV range from 8.7 to 9.4 eV. The theoretical framework is based on anharmonic calculations including overtones and combination bands. The same anharmonic wavenumbers are applied into the simulations of vibrationally-resolved photoelectron spectra based on Franck-Condon computations. The infrared and vibrationally-resolved photoelectron spectra are compared with the available experimental counterparts to verify their accuracy and provide assignment of the observed transitions. Finally, reliable predictions of spectra, going beyond currently available experimental data, either dealing with energy ranges, resolution or temperature, which can support astrochemistry studies are provided.
In-Vitro Characterization of Growth Inhibition against the Gut Pathogen of Potentially Probiotic Lactic Acid Bacteria Strains Isolated from Fermented Products
Authors: Jung Ji Young , Han Sang-Soo , Kim Z-Hun , Kim Myung Hoo , Kang Hye Kyeong , Jin Hyun Mi , Lee Mi Hwa 
Published: October 2021

Lactic acid bacteria (LAB) are probiotic candidates that may restore the balance of microbiota populations in intestinal microbial ecosystems by controlling pathogens and thereby promoting host health. The goal of this study was to isolate potential probiotic LAB strains and characterize their antimicrobial abilities against pathogens in intestinal microbiota. Among 54 LAB strains isolated from fermented products, five LAB strains (NSMJ15, NSMJ16, NSMJ23, NSMJ42, and NFFJ04) were selected as potential probiotic candidates based on in vitro assays of acid and bile salt tolerance, cell surface hydrophobicity, adhesion to the intestinal epithelium, and antagonistic activity. Phylogenetic analysis based on 16S rRNA genes showed that they have high similarities of 99.58–100% to Lacticaseibacillus paracasei strains NSMJ15 and NFFJ04, Lentilactobacillus parabuchneri NSMJ16, Levilactobacillus brevis NSMJ23, and Schleiferilactobacillus harbinensis NSMJ42. To characterize their antimicrobial abilities against pathogens in intestinal microbiota, the impact of cell-free supernatant (CFS) treatment in 10% (v/v) fecal suspensions prepared using pooled cattle feces was investigated using in vitro batch cultures. Bacterial community analysis using rRNA amplicon sequencing for control and CFS-treated fecal samples at 8 and 16 h incubation showed the compositional change after CFS treatment for all five LAB strains. The changed compositions were similar among them, but there were few variable increases or decreases in some bacterial groups. Interestingly, as major genera that could exhibit pathogenicity and antibiotic resistance, the members of Bacillus, Escherichia, Leclercia, Morganella, and Vagococcus were decreased at 16 h in all CFS-treated samples. Species-level classification suggested that the five LAB strains are antagonistic to gut pathogens. This study showed the probiotic potential of the five selected LAB strains; in particular, their antimicrobial properties against pathogens present in the intestinal microbiota. These strains would therefore seem to play an important role in modulating the intestinal microbiome of the host.
Educación superior con nuevas tecnologías de información y comunicación en tiempo de pandemia
Authors: Nivela Cornejo María Alejandrina , Echeverría Desiderio Segundo Vicente , Santos Méndez Marcos Manuel 
Published: October 2021

El objetivo de esta investigación fue describir las herramientas tecnológicas actuales utilizadas en la educación durante el tiempo de confinamiento por pandemia del COVID-19, así como algunos beneficios que ofrecen las nuevas tecnologías de información y comunicación a la educación superior. Se realizó una investigación documental, con diseño descriptivo. La técnica usada fue el análisis de contenido. Se establecieron dos unidades de análisis con sus categorías. Como resultados se destaca que entre las herramientas más usadas están el Learning Magangement System, la realidad virtual, los videojuegos y los gestores de contenido. Entre sus beneficios se mencionan la comprensión, alfabetización digital, autonomía, trabajo colaborativo, pensamiento crítico, flexibilización, motivación y renovación de métodos y procesos de enseñanza. Se concluye que la tecnología es una herramienta imprescindible para la educación superior, las herramientas tecnológicas ayudan a la enseñanza y aprendizaje fundamentalmente en momentos difíciles como los que hoy está atravesando el mundo por la pandemia.
Políticas de la virilidad. Masculinidades en el cine policial y criminal de Carlos Hugo Christensen en Brasil (1960-1970)
Authors: Gil Mariño Cecilia Nuria 
Published: October 2021

Durante varias décadas, la crítica nacional brasileña colaboró con la estigmatización de los géneros cinematográficos identificándolos con el imperialismo cultural y diferenciando a sus realizadores entre autores y artesanos. Esto impactó en el desarrollo de una historiografía que privilegió al cine de autor. No obstante, el cine policial, criminal, el terror y sus monstruos constituyen un espacio discursivo privilegiado a la hora de pensar los miedos, las ansiedades y las formas de violencia de una sociedad en relación con las matrices culturales binarias de las imágenes y discursos de lo masculino y lo femenino.
En este sentido, el presente trabajo aborda la filmografía de Carlos Hugo Christensen en Brasil, referente ineludible de los orígenes del género policial en la Argentina, para indagar la potencialidad política de estos objetos de la cultura de masas desde una perspectiva de género. Se buscará analizar las relaciones entre las desestabilizaciones del orden y la ley como institución masculina que se plantean en el género policial y criminal, con las de la heteronormatividad a través de la puesta en escena de subjetividades que desafían o ponen en cuestión las relaciones de poder de las masculinidades.
A CENTRALIDADE DE MOSSORÓ (RN) NA REDE URBANA-REGIONAL (1858-1915)
Authors: Oliveira Elizangela Justino de 
Published: October 2021

A partir da segunda metade do século XIX, Mossoró torna-se um importante nó da rede urbana potiguar devido a sua função de empório e entreposto comercial entre o litoral e a sertão, cujo raio de influência extrapolava os limites territoriais da província do Rio Grande do Norte, alcançando o Ceará e a Paraíba. A partir da análise da Geografia Histórica Urbana de Mossoró, é possível identificar as diferentes funções e as causas de sua influência sobre o território em diferentes temporalidades. Nesse sentido, este artigo tem como objetivo analisar a centralidade de Mossoró na rede urbana regional em fins do século XIX e início do século XX, identificando, para tanto, a atração e dispersão dos diferentes fluxos a partir da cidade, que lhe atribui centralidade em temporalidades distintas. A pesquisa possui abordagem na Geografia Histórica Urbana, conforme a demarcação temporal e a condução da análise com ênfase em várias fontes documentais primárias. Conclui-se que desde meados do século XIX, Mossoró constitui-se um importante nó da rede urbana-regional, passando por diversos processos de reestruturação de suas funções urbanas.