bluuebunny/crossref_metadata_2025_split huggingface dataset:
Conserved and divergent aspects of Robo receptor signaling and regulation betweenDrosophilaRobo1 andC. elegansSAX-3
Authors: Daiber Trent , VanderZwan-Butler Christine J , Bashaw Greg J , Evans Timothy A 
Published: February 2021

AbstractThe evolutionarily conserved Roundabout (Robo) family of axon guidance receptors control midline crossing of axons in response to the midline repellant ligand Slit in bilaterian animals including insects, nematodes, and vertebrates. Despite this strong evolutionary conservation, it is unclear whether the signaling mechanism(s) downstream of Robo receptors are similarly conserved. To directly compare midline repulsive signaling in Robo family members from different species, here we use a transgenic approach to express the Robo family receptor SAX-3 from the nematode Caenorhabditis elegans in neurons of the fruit fly, Drosophila melanogaster. We examine SAX-3’s ability to repel Drosophila axons from the Slit-expressing midline in gain of function assays, and test SAX-3’s ability to substitute for Drosophila Robo1 during fly embryonic development in genetic rescue experiments. We show that C. elegans SAX-3 is properly translated and localized to neuronal axons when expressed in the Drosophila embryonic CNS, and that SAX-3 can signal midline repulsion in Drosophila embryonic neurons, although not as efficiently as Drosophila Robo1. Using a series of Robo1/SAX-3 chimeras, we show that the SAX-3 cytoplasmic domain can signal midline repulsion to the same extent as Robo1 when combined with the Robo1 ectodomain. We show that SAX-3 is not subject to endosomal sorting by the negative regulator Commissureless (Comm) in Drosophila neurons in vivo, and that peri-membrane and ectodomain sequences are both required for Comm sorting of Drosophila Robo1.
Association of GDF15 With Inflammation and Physical Function During Aging and Recovery After Acute Hospitalization: A Longitudinal Study of Older Patients and Age-Matched Controls
Authors: Tavenier Juliette , Rasmussen Line Jee Hartmann , Andersen Aino Leegaard , Houlind Morten Baltzer , Langkilde Anne , Andersen Ove , Petersen Janne , Nehlin Jan O 
Published: February 2021

Abstract
Growth differentiation factor 15 (GDF15) is a stress-induced cytokine. Its plasma levels increase during aging and acute illness. In older Patients and age-matched Controls, we evaluated whether GDF15 levels (i) were associated with recovery after acute illness, and (ii) reflected different trajectories of aging and longitudinal changes in health measures. Fifty-two older Patients (≥65 years) were included upon admission to the emergency department (ED). At 30 days after discharge (time of matching), Patients were matched 1:1 on age and sex with Controls who had not been hospitalized within 2 years of inclusion. Both groups were followed up after 1 year. We assessed plasma levels of GDF15 and inflammatory biomarkers, frailty, nutritional status (mini nutritional assessment short-form), physical and cognitive function, and metabolic biomarkers. In Patients, elevated GDF15 levels at ED admission were associated with poorer resolution of inflammation (soluble urokinase plasminogen activator receptor [suPAR]), slowing of gait speed, and declining nutritional status between admission and 30-day follow-up. At time of matching, Patients were frailer and overall less healthy than age-matched Controls. GDF15 levels were significantly associated with participant group, on average Patients had almost 60% higher GDF15 than age-matched Controls, and this difference was partly mediated by reduced physical function. Increases in GDF15 levels between time of matching and 1-year follow-up were associated with increases in levels of interleukin-6 in Patients, and tumor necrosis factor-α and suPAR in age-matched Controls. In older adults, elevated GDF15 levels were associated with signs of accelerated aging and with poorer recovery after acute illness.
Hypothesis tests on Rayleigh wave radiation pattern shapes: a theoretical assessment of idealized source screening
Authors: Carmichael Joshua D 
Published: February 2021

SUMMARYShallow seismic sources excite Rayleigh wave ground motion with azimuthally dependent radiation patterns. We place binary hypothesis tests on theoretical models of such radiation patterns to screen cylindrically symmetric sources (like explosions) from non-symmetric sources (like non-vertical dip-slip or non-VDS faults). These models for data include sources with several unknown parameters, contaminated by Gaussian noise and embedded in a layered half-space. The generalized maximum likelihood ratio tests that we derive from these data models produce screening statistics and decision rules that depend on measured, noisy ground motion at discrete sensor locations. We explicitly quantify how the screening power of these statistics increase with the size of any dip-slip and strike-slip components of the source, relative to noise (faulting signal strength) and how they vary with network geometry. As applications of our theory, we apply these tests to (1) find optimal sensor locations that maximize the probability of screening non-circular radiation patterns and (2) invert for the largest non-VDS faulting signal that could be mistakenly attributed to an explosion with damage, at a particular attribution probability. Finally, we quantify how certain errors that are sourced by opening cracks increase screening rate errors. While such theoretical solutions are ideal and require future validation, they remain important in underground explosion monitoring scenarios because they provide fundamental physical limits on the discrimination power of tests that screen explosive from non-VDS faulting sources.
Ileocolonic Healing After Extended Small Bowel Resection in Mice: NOD2 Deficiency Impairs Anastomotic Healing and Postoperative Outcome
Authors: Witte Maria , Reiner Johannes , Bannert Karen , Jaster Robert , Maschmeier Christian , Schafmayer Clemens , Lamprecht Georg , Berlin Peggy 
Published: February 2021

Abstract

Background
Nucleotide-binding oligomerization domain-containing protein 2 (NOD2) mutations are a genetic risk factor for Crohn disease. Ileocecal resection is the most often performed surgery in Crohn disease. We investigated the effect of Nod2 knockout (KO) status on anastomotic healing after extended ileocecal resection (ICR) in mice.

Methods
Male C57BL6/J wild-type and Nod2 KO mice underwent an 11 cm resection of the terminal ileum including the cecum. An end-to-end jejuno-colostomy was performed. Animals were killed after 5 days investigating bursting pressure, hydroxyproline content, and expression of matrix metabolism genes, key cytokines, and histology of the anastomosis.

Results
Mortality was higher in the Nod2 KO group but not because of local or septic complications. Bursting pressure was significantly reduced in the Nod2 KO mice (32.5 vs 78.0 mmHg, P &lt; 0.0024), whereas hydroxyprolin content was equal. The amount of granulation tissue at the anastomosis was similar but more unstructured in the Nod2 KO mice. Gene expression measured by real-time polymerase chain reaction showed significantly increased expression for Collagen 1alpha and for collagen degradation as measured by matrix metalloproteinase-2, -9, and -13 in the Nod2 KO mice. Gelatinase activity from anastomotic tissue was enhanced by Nod2 status. Gene expression of arginase I, tumor necrosis factor-α, and transforming growth factor-ß but not inducible nitric oxide synthase were also increased at the anastomosis in the Nod2 KO mice compared with the control mice.

Conclusions
We found that Nod2 deficiency results in significantly reduced bursting pressure after ileocecal resection. This effect is mediated via an increased matrix turnover. Patients with genetic NOD2 variations may be prone to anastomotic failure after bowel resection.
Interannual variability of gelatinous mesozooplankton in a temperate shelf sea: greater abundance coincides with cooler sea surface temperatures
Authors: Long A P , Haberlin D , Lyashevska O , Brophy D , O’ Hea Brendan , O’Donnell C , Scarrott R G , Lawton C , Doyle T K 
Published: February 2021

AbstractAlthough gelatinous zooplankton are an important component of marine ecosystems, gelatinous mesozooplankton that are &lt;2 cm are underrepresented in monitoring programmes. Here, the interannual variability of gelatinous mesozooplankton abundance and diversity was estimated from 167 zooplankton net samples that were collected in the Celtic Sea during seven fisheries surveys between 2007 and 2019 and analysed alongside environmental parameters. Compositional changes occurred interannually, including an overturn in the abundance ratio of two siphonophores (Muggiaea atlantica and Agalma elegans). Analysis of annual mean gelatinous abundance revealed no linear trend over time (Spearman, r = −0.09, p = 0.287); however, the interannual abundance varied by a factor of 33 (minimum mean abundance in 2013 = 7.36 ± 4.86 individuals m−3; maximum in 2017 = 244.82 ± 84.59 individuals m−3). Holoplanktonic taxa dominated the abundance of the gelatinous community (93.27%) and their abundance was negatively associated with summer sea surface temperature (represented by the 16°C isotherm in July), and the Eastern Atlantic Pattern index 3 months prior (April). Our data suggest that gelatinous mesozooplankton in the Celtic Sea may become less abundant with further ocean warming, and further highlight the need to monitor gelatinous mesozooplankton with a high taxonomic resolution moving forward.
Effects of flavophospholipol on conjugation and plasmid curing of multidrug-resistantSalmonellaEnteritidis in broiler chickens
Authors: Lim Kelvin , Pennell Michael , Lewis Stephanie , El-Gazzar Mohamed , Gebreyes Wondwossen A 
Published: February 2021

AbstractBackgroundEarly in vitro studies suggested that flavophospholipol has plasmid-curing effects and could inhibit conjugation by disrupting pilus formation between bacteria.ObjectivesThis 36-day controlled-challenge study aimed to evaluate the anti-conjugative and plasmid-curing effect of flavophospholipol in vivo on plasmid-mediated antimicrobial resistance (AMR) in MDR transconjugant Salmonella Enteritidis in chickens.MethodsA total of 270-day-old chicks were randomly assigned to four control and four treatment groups with two doses of in-feed flavophospholipol (10 ppm and 64 ppm) and in the presence and absence of ampicillin in drinking water. Chicks were orally challenged with Salmonella Enteritidis with known plasmid-encoded AMR factors. Cloacal swabs were collected on Day 7, 14 and 23. On Day 35, all chickens were euthanized, and caecal tissue and content were collected. Antimicrobial susceptibility testing was done with a panel of 12 antimicrobials and interpreted according to CLSI breakpoints.ResultsFlavophospholipol given in-feed at 64 ppm had an anti-conjugative effect. There was a significant reduction of acquisition of resistance to ampicillin, streptomycin and tetracycline by the recipient strains of Salmonella Enteritidis in treatment groups given flavophospholipol in-feed at 64 ppm (P &lt; 0.05). This was not seen with flavophospholipol given in-feed at 10 ppm.ConclusionsThe results demonstrate that flavophospholipol given in-feed at 64 ppm had an anti-conjugative effect. The results also suggest that AMR is reduced through other mechanisms of action, which are yet to be determined. There is insufficient evidence that flavophospholipol at 64 ppm in feed alone or with sub-therapeutic levels of antibiotics had a plasmid-curing effect.
DDIWAS: High-throughput electronic health record-based screening of drug-drug interactions
Authors: Wu Patrick , Nelson Scott D , Zhao Juan , Stone Jr Cosby A , Feng QiPing , Chen Qingxia , Larson Eric A , Li Bingshan , Cox Nancy J , Stein C Michael , Phillips Elizabeth J , Roden Dan M , Denny Joshua C , Wei Wei-Qi 
Published: February 2021

Abstract

Objective
We developed and evaluated Drug-Drug Interaction Wide Association Study (DDIWAS). This novel method detects potential drug-drug interactions (DDIs) by leveraging data from the electronic health record (EHR) allergy list.

Materials and Methods
To identify potential DDIs, DDIWAS scans for drug pairs that are frequently documented together on the allergy list. Using deidentified medical records, we tested 616 drugs for potential DDIs with simvastatin (a common lipid-lowering drug) and amlodipine (a common blood-pressure lowering drug). We evaluated the performance to rediscover known DDIs using existing knowledge bases and domain expert review. To validate potential novel DDIs, we manually reviewed patient charts and searched the literature.

Results
DDIWAS replicated 34 known DDIs. The positive predictive value to detect known DDIs was 0.85 and 0.86 for simvastatin and amlodipine, respectively. DDIWAS also discovered potential novel interactions between simvastatin-hydrochlorothiazide, amlodipine-omeprazole, and amlodipine-valacyclovir. A software package to conduct DDIWAS is publicly available.

Conclusions
In this proof-of-concept study, we demonstrate the value of incorporating information mined from existing allergy lists to detect DDIs in a real-world clinical setting. Since allergy lists are routinely collected in EHRs, DDIWAS has the potential to detect and validate DDI signals across institutions.
Transforming and evaluating electronic health record disease phenotyping algorithms using the OMOP common data model: a case study in heart failure
Authors: Papez Vaclav , Moinat Maxim , Payralbe Stefan , Asselbergs Folkert W , Lumbers R Thomas , Hemingway Harry , Dobson Richard , Denaxas Spiros 
Published: February 2021

Abstract

Objective
The aim of the study was to transform a resource of linked electronic health records (EHR) to the OMOP common data model (CDM) and evaluate the process in terms of syntactic and semantic consistency and quality when implementing disease and risk factor phenotyping algorithms.

Materials and Methods
Using heart failure (HF) as an exemplar, we represented three national EHR sources (Clinical Practice Research Datalink, Hospital Episode Statistics Admitted Patient Care, Office for National Statistics) into the OMOP CDM 5.2. We compared the original and CDM HF patient population by calculating and presenting descriptive statistics of demographics, related comorbidities, and relevant clinical biomarkers.

Results
We identified a cohort of 502 536 patients with the incident and prevalent HF and converted 1 099 195 384 rows of data from 216 581 914 encounters across three EHR sources to the OMOP CDM. The largest percentage (65%) of unmapped events was related to medication prescriptions in primary care. The average coverage of source vocabularies was &gt;98% with the exception of laboratory tests recorded in primary care. The raw and transformed data were similar in terms of demographics and comorbidities with the largest difference observed being 3.78% in the prevalence of chronic obstructive pulmonary disease (COPD).

Conclusion
Our study demonstrated that the OMOP CDM can successfully be applied to convert EHR linked across multiple healthcare settings and represent phenotyping algorithms spanning multiple sources. Similar to previous research, challenges mapping primary care prescriptions and laboratory measurements still persist and require further work. The use of OMOP CDM in national UK EHR is a valuable research tool that can enable large-scale reproducible observational research.
Cystic lymphangioma in adult—a rare case scenario or a misdiagnosis?
Authors: Sharma J V Pranav , Kazi Farah Naaz 
Published: February 2021

Abstract
Cystic lymphangiomas are a rare entity in adults. It is commonly congenital due to obstruction in the lymphatic drainage. We report the case of a 45-year-old female who was admitted with complaints of a lateral neck swelling associated with multiple palpable cervical lymph nodes. Patient was evaluated and relevant investigations were carried out. Her radiological investigations reported conflicting results of lymphatic cyst, branchial cyst and infected cyst or abscess. However, after an excisional biopsy when the sample tissue was sent for histopathology, the results featured a lymphangioma, which is a rare finding in adults of this age.
A huge ovarian serous cystadenoma with situs inversus totalis: first case report
Authors: Çetin Erman , Öner Eyüp , Aday Ulaş , Güzelgül Mehmet , Gökçe Ayça Orhan 
Published: February 2021

Abstract
Coexistence of situs inversus totalis and ovarian serous cystadenoma in pubertal girls is extremely rare. It is important to preserve ovarian hormonal physiology and fertility if it is detected in the pubertal period.
A 16-year-old girl presented with abdominal distension and pain. Radiological evaluation revealed a huge abdominal cystic mass and situs inversus totalis. In laparotomy, unilateral salpingoophorectomy and total cystectomy were performed on the ovarian cystic mass. It was confirmed as serous cystadenoma in pathological evaluation.
This is the first reported case in the literature of situs inversus totalis with a huge ovarian serous cystadenoma.
Acute retroperitoneal hemorrhage after oocyte retrieval: a case report
Authors: Xu Yangying , Hao Cuifang , Liu Xiaoqiang , Yang Zongzhi , Sun Xianhua 
Published: February 2021

Abstract
A 29-year-old woman with a 5-year history of primary infertility underwent in vitro fertilization-embryo transfer (IVF-ET) treatment. Hemorrhagic shock caused by retroperitoneal hematoma after oocyte retrieval was treated promptly by the evaluation of diagnostic laparoscopy and angiography. The patient was recovered and discharged from the hospital 7 days later without any complications. She was later diagnosed with Von Willebrand disease by a hematologist.
Exploring the Feasibility of Recruiting Respondents and Collecting Web Data via Smartphone: A Case Study of Text-To-Web Recruitment for a General Population Survey in Germany
Authors: Bucher Hannah , Sand Matthias 
Published: February 2021

Abstract
The widespread usage of smartphones, as well as their technical features, offers many opportunities for survey research. As a result, the importance and popularity of smartphone surveys is steadily increasing. To explore the feasibility of a new text-to-web approach for surveying people directly via their smartphones, we conducted a case study in Germany in which we recruited respondents from a mobile random digit dialing sample via text messages that included a link to a web survey. We show that, although this survey approach is feasible, it is hampered by a number of issues, namely a high loss of numbers at the invitation stage, and a high rate of implicit refusals on the landing page of the survey.
Phosphorus fertilization induces nectar secretion for honeybee visitation and cross-pollination of almond trees
Authors: Karunakaran Ranjith , Yermiyahu Uri , Dag Arnon , Sperling Or 
Published: February 2021

AbstractPrecise phosphorus (P) application requires a mechanistic understanding of mineral effects on crop biology and physiology. Photosynthate assimilation, metabolism, and transport require phosphorylation, and we postulated that P is critical for the bloom and fruit-set of almond trees that rely on stored carbohydrate reserves. Hence, we studied the growth, physiology and carbohydrate dynamics in 2-year-old almond trees irrigated with P concentrations between 1 mg l−1 and 20 mg l−1. Almond trees attained maximal photosynthesis, transpiration, and growth by 6 mg P l−1 irrigation. Nevertheless, almond trees continued to extract P in 10 mg P l−1 and 15 mg P l−1 irrigations, which corresponded to larger yields. We attributed the augmented productivity to increased fruit-set (59% between 6 mg P l−1 and 15 mg P l−1), caused by more frequent (29%) honeybee visits. High P improved pollinator visitation by enabling almond trees to utilize more of their starch reserves for nectar secretion (which increased by ~140% between 6 mg P l−1 and 15 mg P l−1). This work elucidates the benefits of P fertilization to plant–pollinator mutualism, critical to almond productivity, and reveals novel indices for optimal P application in almond orchards.
An Analysis of Outcomes and Interventions for Female Pediatric Casualties in Iraq and Afghanistan
Authors: Gale Hannah L , Koons Natalie J , Borgman Matthew A , April Michael D , Schauer Steven G 
Published: February 2021

ABSTRACT

Background
Traumatic injuries were the most common reason for admission of pediatric patients to military hospitals during the recent wars in Iraq and Afghanistan. We compare survival and interventions between female and male pediatric casualties.

Materials and Methods
This is a secondary analysis of a previously described dataset from the Department of Defense Trauma Registry. We requested pediatric encounters from January 2007 to January 2016 within Iraq and Afghanistan. We separated casualties by sex to compare injury and mortality patterns.

Results
Our initial dataset included 3439 pediatric encounters—784 (22.8%) females and 2655 (77.2%) males. Females were less likely to sustain injuries by explosive (38.0% versus 44.5%) but more likely to sustain injuries via alternative mechanisms of injury (28.9% versus 21.5%). Both sexes had similar ISS (females median 10 [5-17], males 10 [4-17]). Fewer females underwent tourniquet application (4.2% versus 7.2%; all findings were significant). In unadjusted and adjusted regression analyses, females under age 8 had lower odds of survival to hospital discharge (OR 0.67, 95% CI 0.51-0.89) compared to males.

Conclusions
Among pediatric patients treated by U.S. medical personnel in Iraq and Afghanistan, females had a lower survival to hospital discharge despite similar severity of injury. Further studies are necessary to elucidate causes for this finding.
Shatter or not: role of temperature and metallicity in the evolution of thermal instability
Authors: Das Hitesh Kishore , Choudhury Prakriti Pal , Sharma Prateek 
Published: February 2021

ABSTRACT
We test how metallicity variation (a background gradient and fluctuations) affects the physics of local thermal instability using analytical calculations and idealized, high-resolution 1D hydrodynamic simulations. Although the cooling function (Λ[T, Z]) and the cooling time (tcool) depend on gas temperature and metallicity, we find that the growth rate of thermal instability is explicitly dependent only on the derivative of the cooling function relative to temperature (∂ln Λ/∂ln T) and not on the metallicity derivative (∂ln Λ/∂ln Z). For most of 104 K ≲ T ≲ 107 K, both the isobaric and isochoric modes (occurring at scales smaller and larger than the sonic length covered in a cooling time [cstcool], respectively) grow linearly, and at higher temperatures (≳107 K) the isochoric modes are stable. We show that even the non-linear evolution depends on whether the isochoric modes are linearly stable or unstable. For the stable isochoric modes, we observe the growth of small-scale isobaric modes but this is distinct from the non-linear fragmentation of a dense cooling region. For unstable isochoric perturbations we do not observe large density perturbations at small scales. While very small clouds (∼min[cstcool]) form in the transient state of non-linear evolution of the stable isochoric thermal instability, most of them merge eventually.
Is the Dinoflagellate Amoebophrya Really Missing an mtDNA?
Authors: Kayal Ehsan , Smith David R 
Published: February 2021

Abstract
Mitochondrial DNA (mtDNA) is a universal hallmark of aerobic eukaryotes. That is why the recent suggestion by John et al. (2019. An aerobic eukaryotic parasite with functional mitochondria that likely lacks a mitochondrial genome. Sci Adv. 5(4):eaav1110.) that the aerobic dinoflagellate Amoebophrya sp. strain AT5 (Syndiniales) lacks mtDNA was so remarkable. Here, by reanalyzing recently published genomic and transcriptomic data from three Amoebophrya strains, we provide evidence of a cryptic, highly reduced mtDNA in this clade. More work is needed before one can definitively say if Amoebophrya has or does not have an mtDNA, but for now, the data are pointing toward the existence of one. Ultimately, we urge caution when basing supposedly absent genomic features on single line evidences.