bluuebunny/crossref_metadata_2025_split huggingface dataset:
mtor Haploinsufficiency Ameliorates Renal Cysts and Cilia Abnormality in Adult Zebrafish tmem67 Mutants
Authors: Zhu Ping , Qiu Qi , Harris Peter C. , Xu Xiaolei , Lin Xueying 
Published: February 2021

Significance Statement
Zebrafish embryos are well recognized for their value in studying polycystic kidney disease, but they cannot fully recapitulate the pathogenesis of the disease. The establishment of tmem67

e3/e3
mutants provides the first adult zebrafish model for cystogenesis. The model exhibits progressive renal cysts and enables the discernment of compensational effects from pathogenic events. Development of a tissue-clearing, whole-mount imaging protocol allows the analysis of renal cysts and cilia abnormality at a single-nephron resolution. Evidence also indicates potential benefit of mammalian target of rapamycin inhibition in tmem67-based cystogenesis. Combining powerful genetics and phenotyping tools, this novel adult zebrafish model shall facilitate mechanistic studies and therapeutic development for cystic diseases.

Background
Although zebrafish embryos have been used to study ciliogenesis and model polycystic kidney disease (PKD), adult zebrafish remain unexplored.

Methods
Transcription activator-like effector nucleases (TALEN) technology was used to generate mutant for tmem67, the homolog of the mammalian causative gene for Meckel syndrome type 3 (MKS3). Classic 2D and optical-clearing 3D imaging of an isolated adult zebrafish kidney were used to examine cystic and ciliary phenotypes. A hypomorphic mtor strain or rapamycin was used to inhibit mTOR activity.

Results
Adult tmem67 zebrafish developed progressive mesonephric cysts that share conserved features of mammalian cystogenesis, including a switch of cyst origin with age and an increase in proliferation of cyst-lining epithelial cells. The mutants had shorter and fewer distal single cilia and greater numbers of multiciliated cells (MCCs). Absence of a single cilium preceded cystogenesis, and expansion of MCCs occurred after pronephric cyst formation and was inversely correlated with the severity of renal cysts in young adult zebrafish, suggesting a primary defect and an adaptive action, respectively. Finally, the mutants exhibited hyperactive mTOR signaling. mTOR inhibition ameliorated renal cysts in both the embryonic and adult zebrafish models; however, it only rescued ciliary abnormalities in the adult mutants.

Conclusions
Adult zebrafish tmem67 mutants offer a new vertebrate model for renal cystic diseases, in which cilia morphology can be analyzed at a single-nephron resolution and mTOR inhibition proves to be a candidate therapeutic strategy.
Compensating Landowners? The State's (Limited) Duty toward Landowners in Delayed Eviction Matters
Authors: Fick Sarah 
Published: February 2021

The unlawful occupation of private land creates a tension between the interests of the unlawful occupiers to avoid homelessness and the landowner to regain control of its property. To balance the interests and rights of the occupiers and the landowners, courts have relied on the municipality, due to its constitutional housing duty, to provide the unlawful occupiers with alternative accommodation. The provision of alternative accommodation prevents homelessness, while at the same time allowing the landowner to regain control of its property.
In response to demands by unlawful occupiers that they provide alternative accommodation, municipalities have either failed to cooperate or argued that they are unable to accommodate the unlawful occupiers immediately due to resource constraints. This has led to delays in eviction matters to the detriment of landowners. Only in one delayed eviction matter did the Constitutional Court order relief for the landowner. It ordered the municipality to pay constitutional damages to the landowner. After this order, scholars have advocated for similar relief to be granted in other delayed eviction matters. This article aims to add to that debate, by determining under what circumstances an award of constitutional damages in such matters would be appropriate, just and equitable, as is required of a constitutional remedy.
Fatores relacionados da proposição diagnóstica atraso no desenvolvimento em adolescentes escolares: estudo transversal
Authors: Nogueira Isadora Lorenna Alves , Gabriel Maynara Caroline Gomes , Lúcio Kadyjina Daiane Batista , Carino Ana Carolina Costa , Lopes Marcos Venícios de Oliveira , Lira Ana Luisa Brandão de Carvalho 
Published: February 2021

Problema: A ausência de estímulos facilitadores no ambiente em que o adolescente convive é normalmente atribuída a ocorrência do atraso do desenvolvimento. Objetivo: Analisar a associação dos fatores relacionados com a proposta diagnóstica atraso no desenvolvimento em adolescentes no ambiente escolar. Método: Estudo transversal, realizado com 385 adolescentes em uma cidade no Nordeste do Brasil. Resultados: A prevalência da proposição diagnóstica atraso no desenvolvimento foi 18,26%. Os fatores relacionados que associação associação associados foram: marginalização social, dor crônica, não aceita às transformações corporais da puberdade e trauma psicológico. Discussão:Percebe-se a relação entre as variáveis estudadas e o desenvolvimento humano frente à literatura. Conclusão: O conhecimento dos fatores associados mais frequentes nessa população, facilita a inferência da proposição diagnóstica pelo enfermeiro. Outrossim, contribui na identificação de aspectos capazes de gerar prejuízos ao adolescente e, consequentemente, sequelas na fase adulta.
The effect of free radical stress correction on corticoid signaling in the kidney of rat with different resistance to hypoxia after systemic circulation arrest
Authors: Bayburina G A , Samigullina A F , Drozdova G A 
Published: February 2021

Aim. To assess the influence of the pathogenetic action of the succinate-containing drug on corticosteroid regulation in the kidney of rats with different resistance to hypoxia during recovery after systemic circulation arrest.
Methods. The object of the study was male non-inbred white rats weighing 200220 g. A week after testing for resistance to hypoxia, a 5-minute systemic circulation arrest was simulated by intrathoracic clamping of the vascular bundle of the heart, followed by resuscitation. In the postresuscitation period, the experimental rats were once daily injected with a solution containing inosine + nicotinamide + riboflavin + succinic acid, and the control rats 0.9% Sodium Chloride solution. The observation period was 35 days. We studied the content of corticosterone, aldosterone in blood plasma, gluco- and mineralocorticoid receptors, carbonylated proteins, dityrosine, and products that react with thiobarbituric acid in homogenates of the kidneys. Statistical data were presented as mean and standard deviation M. Nonparametric KruskalWallis (N) and MannWhitney (U) tests followed by Dunn test, Spearman correlation analysis were used. Differences were considered statistically significant at p 0.05.
Results. The use of succinate-containing preparation reduced the intensity of free radical processes in both groups of animals. Against this background, in low-resistance rats, on the 1st day, the concentration of glucocorticoid receptors statistically significantly increased to 117% (p 0.05), and then was comparable to the control; the greatest statistically significant changes in the level of mineralocorticoid receptors occurred on the 1st day (increase by 25%, p 0.001) and at 2135th days (decrease by 2230%, p 0.001). In highly resistant rats, the correction led to a shift in the maximum content of glucocorticoid receptors from the last day (134% of the control level, p 0.01 without therapy) to the 1st (123%, p 0.05 with succinate-containing therapy) and maintaining the receptors level comparable to the initial, in the future. The level of mineralocorticoid receptors in highly resistant rats was lower than in low resistant rats, both in the group without correction and with correction.
Conclusion. Correction of the course of the postresuscitation period with a succinate-containing drug in animals with a low resistance to hypoxia against the background of a decrease in the intensity of carbonyl stress and restoration of feedback mechanisms causes stabilization of glucocorticoid receptors level and a decrease in mineralocorticoid receptors to control values by the end of the experiment; in organisms highly resistant to hypoxia, against the background of correction, the activity of lipid peroxidation decreases and the level of both types of receptors are restored.
Ecos da violência na literatura brasileira contemporânea
Authors: Ribeiro Rondinele Aparecido 
Published: February 2021

<p>A literatura contemporânea é marcada por diversas possibilidades de escrita, sobressaindo, portanto, uma multiplicidade de formas, que não necessariamente precisam se sobrepor uma a outra, mas sim conviverem de maneira harmônica. Como uma das possibilidades expressivas desse mosaico artístico, destaca-se a violência, vertente relevante na configuração do cenário literário nacional devido sua imanência à constituição do país. Dessa forma, intentamos com este artigo, analisar como se configura essa temática em alguns textos da literatura mais recente.</p><p><strong> </strong></p>
O imaginário feminino sobre o casamento em Gil Vicente: “libertação ou fardo?”
Authors: Mendes Renata de Jesus Aragão 
Published: February 2021

<p>Este artigo tem como intuito compreender a imagem que as mulheres portuguesas tinham do casamento no século XVI por meio das representações femininas em Gil Vicente. A análise se circunscreve a três peças2 do teatrólogo, a saber, o <em>Auto da Sibila Cassandra</em>, Quem<em> tem farelos?</em> e a <em>Farsa de Inês Pereira</em>. Além disso, fazemos uso da historiografia sobre as mulheres do período medieval e renascentista que tratam do discurso construído sobre o feminino pela literatura clerical. Ambos estes autos nos apresentam tipos femininos com comportamentos verossímeis, ao mesmo tempo em que apontam diferentes imagens sobre omatrimônio no contexto português do século XVI. Gil Vicente nos apresenta duas faces do casamento. Por um lado, é visto enquanto fardo e por outro enquanto libertação. Estas imagens são indicativos que o matrimônio estava cada vez mais perdendo o caráter sagrado defendido tanto pelo poeta quando pela Igreja Católica. Logo, temos como principal objetivo discutir de que maneira estas imagens representadas pelo devoto teatrólogo, nos ajudam a pensar nas questões de gênero dentro das relações matrimoniais e consequentemente no papel assumido pelas mulheres naquele contexto.<br />Palavras-chave: Gênero. Matrimônio. Gil Vicente. Portugal</p>
Paisagens de negação: a ambiência e a melancolia como reflexo da negação em Paisagens de inverno, de Camilo Pessanha
Authors: Santos Ezequias Da Silva 
Published: February 2021

<p>O objetivo desse artigo é analisar os poemas <em>Paisagens de inverno</em> (I e II) de Camilo Pessanha numa tentativa de aproximar o conceito de paisagem, aplicado por Fernando Pessoa em sua obra poética, como reflexo do ambiente negativo/melancólico inerente nos dois poemas supracitados. Para tal propósito, iremos nos ancorar em dois principais textos teóricos que afiançam nosso estudo. São eles: <em>Atmosfera, Ambiência, Stimmung</em>: sobre um potencial oculto na literatura, de Hans Gumbrecht e <em>Melancolia,</em> de Luiz Costa Lima. </p><p><strong> </strong></p>
L’espace narratif comme lieu d’exaltation du theme de la folie dans La case du commandeur d’édouard Glissant
Authors: Debibakas Audrey 
Published: February 2021

<em>La Traite</em> est proprement un "parler indicible" et ne donne lieu à aucun récit. C’est donc sur ce fond d’absence de mythe et d’épopées que s’inscrit <em>La Case du commandeur</em>, d’Edouard Glissant. Le roman tente de retranscrire la complexité de la remontée généalogique et mémorielle à travers une quête des origines à rebours. Il insiste sur la dimension lacunaire fragmentée de l’histoire en mettant en avant une pensée du vide et de l’indicible, notamment dans la section centrale « le Trou du temps ». Cependant, loin de se focaliser sur l’exil et le déracinement, le romanpermet l’instauration d’un « lieu <em>digénique</em> » c’est-à-dire d’un nouveau lieu narratif: il s’agit d’une dématérialisation de l’espace spatio-temporel. En effet, le roman propose des espaces de (re)création en s’éloignant de toute recherche chronologique et en prônant les possibilités narratives qui naissent de l’instauration de lieux alternatifs. L’écriture achemine les personnages vers des "solutions" narratives ouvertes, échappatoires de l’enfermement et de l’ignorance. Le "non-monde" initial laisse la place à l’émergence. C’est à partir de ces lieux narratifs dématérialisés – entre les vivants et les morts, entre le passé et le présent – dans ces interstices, que les personnages peuvent se "ré-ancrer" à une collectivité qui possède son propre passé et son propre système de représentation historique. Cet espace dématérialisé devient un moyen par lequel la narration transgresse ses frontières textuelles. A ce titre la narration dans <em>La Case du commandeur</em> devient un espace interstitiel grâce auquel le roman parvient malgré tout à décrire des invisibles et des indicibles de la mémoire. Non contente d’en décrire la vacance, le roman transforme la béance historique et mémorielle en atout littéraire et poétique.
Édouard Glissant, une parole ouverte sur le monde
Authors: Danglades Mylène 
Published: February 2021

<span>Édouard Glissant, dans son<em> Traité du Tout-monde</em> paru en 1997 nous propose sa vision d'un monde changeant qui oriente la pensée, le regard humain et génère par-là même une parole ouverte mettant en exergue des identités culturelles inédites. Cette parole cherche à extirper des miasmes de la conscience, du chaos de la mémoire une histoire créatrice. Il s'agit, par le biais de l'écriture, de la langue ou des langues, de percevoir le mystère du vivant ou d'en approcher. </span><span lang="EN-US">Mais l’humain dans “un monde chaos” peut-il se raccrocher à une identité stable en lien avec sa nation, sa langue, sa race, sa religion ou son ethnie? </span><span>Autrui ne s’inscrit-il pas contre son gré dans ce scénario? La relation à l’autre ne vient-elle pas remettre en question ces fondamentaux, ce principe unique? L’Altérité s’organise autour d’un “je” et d’un “nous, d’un jeu entre ces différentes entités, entérinées ou tenues à distance. La poétique de la relation repose sur une opposition que l’auteur qualifie de “bénéfique”, mais si nous sommes plongés dans l’obscurité, en proie à de mystérieuses sensations, mais il importe avant tout d’extraire de tout cela “de nouvelles possibilités créatrices”.</span>
Um estudo de caso sobre o ensino de inglês por meio da abordagem (ESP)
Authors: Silva Suellen Cordovil da , Silva Maria Auxiliadora da 
Published: February 2021

<p>O objetivo geral desta pesquisa é investigar a coerência do ensino da Língua Inglesa por meio da abordagem ESP, nos cursos ofertados pelo Idioma sem Fronteiras na UNIFESSPA, no qual tem como público alvo os discentes, estes que necessitam do inglês para propósitos acadêmicos como para pleitear programas de mobilidade acadêmica. Este estudo se direciona a delimitar alguns conceitos sobre os processos da internacionalização na educação superior, a importância da Língua Inglesa a nível internacional e o seu ensino para fins específicos de acordo com os autores: Kirsch (2015), Lamri (2016), Gattolin e Navarro (2016), Vilaça (2003), entre outros; a análise das novas políticas linguísticas e suas possíveis reflexões voltadas para a implantação de uma nova política linguística e sobre o desenvolvimento do programa idiomas sem fronteiras na UNIFESSPA, a partir do levantamento de dados dos quais serão: observações de aulas no NucLi, entrevista com as professoras bolsistas e a coerência do material utilizado em relação ao EPS no ensino do inglês para finalidades específicas.</p><p> </p>
Perspectivas de eCommerce y los HÃ¡bitos de Consumo Tras COVID-19
Authors: Vega Barrios Alejandra , CerÃ³n Islas Arlen , Figueroa VelÃ¡zquez Juan Gabriel 
Published: February 2021

El presente artÃ­culo tiene como finalidad estudiar las tendencias de compra en lÃ­nea dadas las condiciones actuales de aislamiento que se establecieron por la pandemia COVID-19; por lo que se realiza un anÃ¡lisis cualitativo basado en la metodologÃ­a de la TeorÃ­a Fundamentada, con la identificaciÃ³n y categorizaciÃ³n de variables sobre los hÃ¡bitos de consumo y la experiencia de la compra por Comercio ElectrÃ³nico (eCommerce). Para la construcciÃ³n de este trabajo se realizaron entrevistas semiestructuradas a consumidores en la ciudad de Pachuca, Hidalgo; quienes hicieron compras por comercio electrÃ³nico en el periodo comprendido de marzo a agosto del aÃ±o 2020, durante el confinamiento. Los resultados obtenidos en esta primera etapa proporcionan informaciÃ³n para la incorporaciÃ³n del eCommerce como parte de las estrategias de exposiciÃ³n y venta de productos para el cambio en los hÃ¡bitos de consumo dada la â€œnueva normalidadâ€.

The purpose of this article is to study ECommerce trends between conditions of isolation established by the COVID-19 pandemic. The research was done by a qualitative analysis, that is performed based on the Grounded Theory methodology with the identification and categorization of variables on consumption habits and the experience of buying by eCommerce. Semistructured consumer interviews were conducted for the construction of this work in the city of Pachuca, Hidalgo; the customers have made purchases by eCommerce in the period from March to August 2020, during the confinement. The results obtained in this first stage provide information for the incorporation of eCommerce as part of sales strategies products in the change in consumption habits given the "new normal".
CaracterizaciÃ³n de la IntenciÃ³n Emprendedora en Proyectos Universitarios con Base a un Evento AcadÃ©mico en Tiempos de COVID-19
Authors: Camacho Genesis HernÃ¡ndez , Favela RodrÃ­guez JosÃ© Luis , Bustamante Arneros Cristian Antonio , Ramos de la Rosa Sandra VerÃ³nica , RodrÃ­guez Gabriela Velasco 
Published: February 2021

En la presente investigaciÃ³n se retoma a la iniciativa emprendedora en proyectos universitarios que â€œrepresenta un indicador de esfuerzo individual o grupal y de la disposiciÃ³n a ejecutar un comportamiento cuando sea el momento y la oportunidad adecuadaâ€ (Krauss Delorme, Bonomo Odizzio, & Volfovicz Leon, 2018, pÃ¡g. 84) ante tiempos de COVID-19. El objetivo es distinguir las caracterÃ­sticas de la intenciÃ³n emprendedora en proyectos de universitarios con base a un evento acadÃ©mico. La metodologÃ­a aplicada consiste en un anÃ¡lisis de 83 proyectos a travÃ©s una encuesta digital en el evento EmprendÃ­zate 2020-1. Con este insumo se plantea los resultados de tres indicadores correspondientes al perfil del emprendedor, sector y giro econÃ³mico, asÃ­ como aquellos factores que contribuyan a la intenciÃ³n emprendedora del proyecto, donde se pone de manifiesto que la interacciÃ³n con otras personas e internet son la fuente de inspiraciÃ³n. En cambio, los inconvenientes estÃ¡n asociados a la disponibilidad de financiamiento, contar con capacitaciÃ³n y de recursos humanos idÃ³neos para la iniciaciÃ³n de los proyectos universitarios.

This research takes up the entrepreneurial initiative in university projects that "represents an indicator of individual or group effort and the willingness to execute a behavior when the time and opportunity are right" in times of COVID-19. This paper focuses on distinguishing the characteristics of the entrepreneurial intention in university projects based on an academic event. The applied methodology consists of an analysis of 83 projects through a digital survey at the EmprendÃ­zate 2020-2021 event. With this input, the results of three indicators corresponding to the profile of the entrepreneur, sector, and economic line are presented. This was in addition those factors that contribute to the entrepreneurial intention of the project. Here it is shown that interaction with other people and the internet are the major source of inspiration. Instead, the drawbacks are associated with the availability of financing and having training and suitable human resources for the initiation of university projects.
Estrategias de Mercadotecnia GastronÃ³mica para Restaurantes de Puebla en Tiempos de Pandemia por COVID-19. Estudio de Caso: GeneraciÃ³n Z
Authors: CerÃ³n Carrillo Teresa Galdys , Morales Paredes Yesbek RocÃ­o , Santiesteban LÃ³pez Norma AngÃ©lica 
Published: February 2021

La pandemia por COVID-19 ha propiciado que los restaurantes en la Ciudad de Puebla reduzcan el flujo de clientes, por lo que implementar las nuevas disposiciones de higiene y sanidad marcadas en el Protocolo para la implementaciÃ³n de medidas preventivas ante COVID-19 en restaurantes de la SecretarÃ­a de protecciÃ³n civil y gestiÃ³n integral de riesgos de la Ciudad de Puebla y el Protocolo de prevenciÃ³n COVID-19 â€œMesa seguraâ€ de la CANIRAC, resulta imprescindible para la reapertura en la nueva normalidad, asÃ­, el uso de estrategias de mercadotecnia gastronÃ³mica se consolida como el medio adecuado para atraer a clientes de la GeneraciÃ³n Z que buscan experiencias seguras, novedosas, saludables y de bajo impacto ambiental, que les brinden valor por lo que pagan, utilizando las herramientas virtuales que se adapten a sus necesidades y gustos. Para lo anterior se realizÃ³ un estudio transversal, no experimental y un cuestionario en lÃ­nea de 20 Ã­tems el cual se distribuyÃ³ vÃ­a redes sociales entre 616 participantes pertenecientes a la GeneraciÃ³n Z, logrando asÃ­ el objetivo de generar estrategias de mercadotecnia gastronÃ³mica para un consumo seguro en restaurantes de Puebla frente a la nueva normalidad. Los resultados obtenidos revelaron que el segmento mencionado estÃ¡ dispuesto a consumir en restaurantes siempre y cuando se cuenten con las medidas de seguridad e higiene propuestas por las autoridades correspondientes, asÃ­ como, cumplan con sus expectativas y exigencias.

The COVID-19 pandemic has led restaurants in Puebla City to reduce the flow of customers. This is why it is essential to implement the new hygiene and health provisions following the Protocol for the preventive measures against COVID-19 in restaurants as proposed by the Ministry of Civil Protection and Integral Risk Management of Puebla as well as CANIRACâ€™s COVID-19 "Safe Table" Prevention Protocol. The use of gastronomic marketing strategies is consolidated as the appropriate means to attract Generation Z clients who are looking for safer, innovative, healthy and lowenvironmental impact experiences that provides value for what they pay, while using virtual tools that adapt to their needs and tastes. Based on the aforementioned, a cross-sectional and non-experimental study was carried out, and an online questionnaire of 20 items was distributed via social networks among 616 participants belonging to Generation Z. This paper focuses on generating gastronomic marketing strategies for safer consumption in Puebla restaurants that have adopted the new normal. Results obtained revealed that this segment are willing to eat in restaurants as long as they have safety and hygiene measures proposed by the corresponding authorities, in addition to meeting their expectations and demands.