bluuebunny/crossref_metadata_2025_split huggingface dataset:
Angelicin Shows Antitumor Effects On The Oral Squamous Cell Carcinoma By Negatively Regulating DUSP6-Mediated cMYC-MAPK Signaling Pathway
Authors: Liu Na , Shang Qianhui , Qi Jiajia , Li Qionghua , Sun Yutong , Chen Jinhuan , Deng Jing , Dan Hongxia , Xie Liang , Chen Qianming 
Published: July 2021

Abstract
Background: Angelicin has been reported to have antitumor effects on many cancers. However, few studies on angelicin in oral squamous cell carcinoma (OSCC) have been performed. Methods: In this study, we performed a cell cycle and apoptosis assay, wound healing assay, and transwell assay to assess the effects of angelicin on malignant phenotypes of OSCC in vitro. To determine the potential regulatory mechanism, we conducted differentially expressed genes analysis of OSCC cells. Subsequently, nude mouse xenograft models were used to evaluate the function of angelicin and validate the regulatory mechanism in vivo. Results: The results showed that angelicin inhibited the malignant phenotype of OSCC in vitro and reduced tumor formation in vivo. Mechanistically, angelicin induced the downregulation of the significantly different gene—dual-specificity phosphatase 6 (DUSP6) and the downstream transcription factor c-MYC in the mitogen-activated protein kinase (MAPK) signaling pathway. Conclusion: Our results indicate that angelicin has antitumor effects on OSCC by negatively regulating the DUSP6-mediated cMYC-MAPK signaling pathway and is a promising antitumor drug in OSCC therapy.
Adiponectin Ameliorates Lung Ischemia-Reperfusion Injury Through SIRT1-Pink1 Signaling-Mediated Mitophagy in Type 2 Diabetic Rats
Authors: Jiang Tao , Liu Tianhua , Deng Xijin , Ding Wengang , Yue Ziyong , Yang Wanchao , Lv Xiangqi , li wenzhi 
Published: July 2021

Abstract
BackgroundDiabetes mellitus (DM) is a key contributing factor to the poor survival in lung transplantation recipients. Mitochondrial dysfunction is recognized as a critical mediator in the pathogenesis of diabetic lung ischemia-reperfusion (IR) injury. The protective effects of adiponectin have been demonstrated in our previously study, but the underlying mechanism remained unclear. Here we demonstrated an important role of mitophagy in the protective effect of adiponectin during diabetic lung IR injury.Methods High-fat diet-fed streptozotocin-induced type 2 diabetic rats as recipients were exposed to adiponectin with or without administration of the SIRT1 inhibitor EX527 following lung transplantation. To unravel the mechanisms underlying the action of adiponectin, rat pulmonary microvascular endothelial cells were transfected with SIRT1 small-interfering RNA or Pink1 small-interfering RNA and then subjected to in vitro diabetic lung IR injury.ResultsMitophagy was impaired in the diabetic lung subjected to IR injury, accompanied by increased oxidative stress, inflammation, apoptosis, and mitochondrial dysfunction. Adiponectin induced mitophagy and attenuated subsequent diabetic lung IR injury by improving lung functional recovery, suppressing oxidative damage, diminishing inflammation, decreasing cell apoptosis, and preserving mitochondrial function. However, both inhibitors of mitophagy and knockdown of Pink1 suppressed mitophagy, and reduced the protective action of adiponectin. Furthermore, we demonstrated that APN affected Pink1 stabilization via the SIRT1 signaling pathway, and knockdown of SIRT1 suppressed Pink1 expression and compromised the protective effect of adiponectin.ConclusionThese data demonstrated that adiponectin attenuated reperfusion-induced oxidative stress, inflammation, apoptosis and mitochondrial dysfunction via activation of SIRT1-Pink1 signaling-mediated mitophagy in diabetic lung IR injury.
Serum Uric Acid as a Potential Biomarker in Patients With Rheumatoid Arthritis-associated Interstitial Pneumonia (RA-ILD)
Authors: Wang Zitao , Wang Wen , Xiang Ting , Gong Bangdong , Xie Jianmin 
Published: July 2021

Abstract
Background: Uric acid (UA) has been reported participate in various inflammatory and autoimmune diseases. Increasing evidence has shown that UA also plays an important role in lung inflammation and fibrosis. We aimed to investigate the correlation between UA and rheumatoid arthritis (RA), especially rheumatoid arthritis-associated interstitial lung disease (RA-ILD).Methods: 266 RA patients and 138 healthy individuals were recruited in this study. RA was identified according to ACR/EULAR 2010 criteria. UA in serum and bronchoalveolar lavage fluid (BALF), as well as clinic and laboratory Indexes were collected from participants enrolled in the study. Serum KL-6 was measured via ELISA and then Spearman correlation analysis was used to analysing their association. Subsequent the receiver operating characteristic (ROC) curve and area under the curve (AUC) were used to estimate the diagnostic value of UA in RA-ILD and UIP pattern of RA-ILD. HRCT and pulmonary function tests (PFT) were performed to evaluated the characteristics and pulmonary findings of RA patients. Furtherly, we validated the effect of UA on the EMT and the expression of cytokines in A549 cells. Results: Compared with healthy controls (HC), UA levels in serum was significantly higher in RA group (262.25±91.94 vs. 234.69±54.78µmol/L, P<0.01). Spearman correlation analysis revealed associations of SUA with risk factors of RA, including RF, anti-CCP, CRP and ESR (r=0.37, p=0.02; r=0.48, p<0.01; r=0.26, p<0.01; r=0.27, p<0.01). And higher UA was measured both in serum (291.81±102.42 vs. 252.38±6.15µmol/L, P<0.01) and BALF (393.3±222.6 vs. 204.0±120.3µmol/L, p<0.01) of RA-ILD patients, particularly those with UIP pattern (475.58±249.28 vs. 262.86±103.45µmol/L, p<0.01; 393.3±222.6 vs. 204.0±120.3µmol/L, p<0.01). Meanwhile, the correlation between the level of UA in serum and BALF and serum KL-6 concentration in RA were also significant (r=0.59, p<0.01; r=0.43, p<0.01). Also, the negative correlations of UA level, both in serum and BALF, with lung function parameters including FEV1/FVC and FEV1% predicted were measured (r=-0.78; r=-0.87, p<0.01). In the ROC curve analysis of AUC, the AUC value of UA was 0.76 (95%CI=0.66-0.87, p<0.01). The sensitivity and specificity were 60% and 92%, respectively. The vitro experiment showed that UA stimulated the EMT in A549 cells, as well as induced the expression of cytokines, such as IL-1, IL-6 and TGF-β, in lung epithelial cell.Conclusions: This study suggests that UA is correlated with the ILD in RA. Particularly, the higher UA levels may be related to UIP, a pattern with worse prognosis, in patients with ILD. Therefore, UA may be an important contributing factor to the pathogenesis of RA-ILD.
Polydatin Protects Against Articular Cartilage Degeneration by Regulating Autophagy Mediated by AMPK/Mtor Signaling Pathway
Authors: Ye Zhengcong , He Chun , Yu Pengzheng , Cao Guoping , Shen Qinrong , Wang Canfeng 
Published: July 2021

Abstract
Background: Knee osteoarthritis (KOA) is one of the leading causes of disability, and its etiopathogenesis is not completely understood. Polydatin has the potential effect on the treatment of KOA, but the mechanism is not clear.Methods: After an KOA rat model was established by anterior cruciate ligament transection, KOA rats were treated with polydatin (4 mg/kg) for 30 days. Subsequently, cartilage tissues were collected from rats and detected by HE, TUNEL staining and Western blotting to evaluate the pathological damage, apoptosis and autophagy activity. Then, human chondrocyte C28/I2 cells were stimulated by LPS to induce a KOA model in vitro, and the effects of polydatin on the C28/I2 cell viability, apoptosis and autophagy were also detected. In addition, the mechanism of polydatin on KOA in C28/I2 cells was investigated, and the effect of an AMPK inhibitor (Dorsomorphin 2HCl) on the proliferation and apoptosis of polydatin administrated-cells were also detected. Results: After treated with polydatin, the pathological damage of rat cartilage tissues were ameliorated, cells apoptosis was inhibited and autophagy was activated in KOA rats. Meanwhile, polydatin also ameliorated the proliferation and apoptosis of C28/I2 cells, the expression of autophagy-related proteins, LC3II/LC3I, Beclin-1, and p-AMPK/AMPK were up-regulated, p-mTOR/mTOR was down-regulated by polydatin in C28/I2 cells. Interestingly, relative results showed that the improvement effect of polydatin on LPS-sdtimulated-C28/I2 cells was blocked by AMPK/mTOR inhibitor, Dorsomorphin 2HCl. Conclusion: Our research showed that polydatin reduces apoptosis and activate autophagy both in a rat model of KOA and C28/I2 cell model by AMPK/mTOR signaling pathway, which provides the basis for further investigations into the potential therapeutic impact of polydatin in KOA.
Glacial Lake Outburst Floods Simulation in the Yi’ong Zangbo River Basin Based on One-Dimensional Hydrodynamic Model
Authors: Wang Qi , Duan Hongyu , Liu Na , Du Zhishui , Wang Pan , Yi Bo , Xu Jun , Huang Jian 
Published: July 2021

Abstract
Glacial lake outburst floods (GLOFs) are a serious potential threat to the safety of life and property in downstream areas. In this study, moraine-dammed glacial lakes in the Yi’ong Zangbo River basin were recognized based on Landsat ETM+/TM/OLI images in 2000 and 2019. And the GLOFs for the Jionglaco, the largest glacial lake in this basin, was simulated using the one-dimensional hydrodynamic model. The results show that the total number and area of moraine-dammed glacial lakes in this basin increased by 10 (10.52%) and 5.49 km2 (48.24%) from 2000 to 2019, in which the area of the Jionglaco increased by 3.22 km2. The peak discharge at the breach outlet for five scenarios with different combinations of breach width (80 m and 120 m), depth (2.5 m and 5 m) and flood time (1.5 h and 3 h) are 489 m3/s, 1327.43 m3/s, 444.32 m3/s, 617.47 m3/s and 1570.61 m3/s. With the addition of baseflow in river, the peak discharge at bridge site 15 138.93 km from Jionglaco are 1040.89 m3/s, 1724.00 m3/s, 1024.85 m3/s, 1162.25 m3/s and 1990.52 m3/s. The combination of baseflow in river and the GLOFs discharge results in the increasing peak discharge in the further downstream region. However, the arrival of peak discharge in downstream areas is delayed, which increases the chances of people escaping. This study aims to provide some references for the prevention of GLOFs in this region.
Effects of dietary Lasia spinosa Thw. powder supplementation on growth performance, blood metabolites, antioxidant status, intestinal morphology, and cecal microbiome in Chinese yellow-feathered broilers
Authors: Yongxing Hong , Lang Zhang , Kui Tian , Haodong Sun , Xingting Liu , Yanfei Tang , A. Hassanin Abdallah , A. Abdelnour Sameh , Yuying Liao , Wanwipa Suthikrai , Kittiya Srisakwattana , Theerawat Tharasanit , Lu Yangqing 
Published: July 2021

Abstract
Background: Lasia spinosa Thw. (LST) has been proven to be nutritious and have growth-promoting, antioxidant functions and so on, but its effect in chicken is still unclear. This study aimed to evaluate the effects of dietary LST powder supplementation on growth performance, blood metabolites, antioxidant status, intestinal morphology and cecal microbiome in Chinese yellow-feathered broilers.Methods: A total of 400 one-day-old yellow-feather broilers were randomly allotted to 4 dietary treatments: LST0 group (a basal diet), LST1 group (a basal diet with 1% LST powder), LST2 group (a basal diet with 2% LST powder), LST4 group (a basal diet with 4% LST powder), ten replicates for each treatment and 10 broilers in each treatment group. Results: Results indicated that the average daily feed intake of broilers during 22-42d and the average daily gain of chickens over all periods were significantly increased by dietary supplementation of LST powder compared to a control group, while the feed conversion ratio during the overall periods was markedly decreased. The levels of SOD, CAT and GSH-Px in serum, liver and breast muscle were also significantly increased in LST supplemented groups, while ROS and MDA in serum, liver and breast muscle were decreased. Furthermore, the levels of TG and LDL-C were significantly decreased by the addition of dietary LST powder, while levels of HDL-C, Ca, Fe, Mg and P were linearly increased. Regarding the gut morphometric, crypt depth was significantly decreased by LST supplementation, while villus height and the ratio of villus height to crypt depth were notably increased. Sequencing of 16S rRNA from the cecal contents of broilers revealed that the composition of the chicken gut microbiota was altered by LST supplementation. Moreover, the diversity of microbiota in broilers was increased in the LST1 groups but was decreased in the LST2 and LST4 groups compared with LST0 groups. The differential genera enriched in LST1 groups, such as Bacillus, Odoribacter, Sutterella, Anaerofilum, Peptococcus, were closely related to the increased growth performance, antioxidant status, intestinal morphology, Ca, Mg and reduced blood lipid in the treated broilers. Conclusions: The supplementation of LST powder to the diets of Chinese yellow-feathered broilers improved growth performance, lipid profile, antioxidant indices, intestinal morphology and gut microbiota balance, with its optimum level in yellow-feathered broilers’ diet being 1%.
Lorenz-invariant system with quantum mechanical properties
Authors: Pérez Francisco de Luis 
Published: July 2021

Abstract
In this work we study potential fluids, within which eddies exist which have quantum
mechanical properties because according to Helmholtz they are made up of an integer
number of lines and their displacement in a potential medium is a function of a frequency.
However, this system is Lorenz-invariant since Maxwell’s equations can be obtained
from it, and this is what we demonstrate here. The considered hypothesis is that the
electric charge arises naturally as the intensity of the eddy in the potential fluid, that is,
the circulation of the velocity vector of the elements that constitute it, along that potential
(it is not another parameter, whose experimental value must be added, as proposed by the
standard model of elementary particles). Hence, the electric field appears as the rotational
of the velocity field, at each point of the potential medium, and the magnetic field appears
as the variation with respect to the velocity field of the potential medium, which is
equivalent to the Biot and Savart law. From these considerations, Maxwell’s equations
are reached, in particular his second equation which is the non-existence of magnetic
monopoles, and the fourth equation which is Ampere’s law, both of which to date are
obtained empirically demonstrated theoretically. The electromagnetic field propagation
equation is also arrived at, thus this can be considered a demonstration that a potential
medium in which eddies exist constitutes a Lorenz-invariant with quantum mechanical
properties.
Impact of Landscape and Season on the Ecological Distribution of Tabanidae and Stomoxyinae, Mechanical Vectors of Bovine Trypanosomosis in the Forest of Sanaga Maritime and Savanna of Ngaoundere, Cameroon
Authors: Djonguep Andre Sieumeni , Mamoudou Abdoulmoumini , Hiol Victor Dermy , Lebalé Oumarou , Sevidzem Silas Lendzele , Kohagne Lisette Tongue , Cornel Adler , Nukenine Elias Nchiwan 
Published: July 2021

Abstract
Background: Huge pasture areas in sub-Saharan Africa are heavily infested by tabanids and stomoxyines whose distribution across season and in different landscape differs and is poorly described in Cameroon. Methods: A longitudinal entomological prospection (January 2017 to December 2017) was carried out in the savanna of Ngaoundere and forest of the Sanaga Maritime of Cameroon, using three trap-types (Nzi, Biconical and Vavoua). A total of 18 traps were used in this study with nine in each site.Results: In the forest, 44317 Stomoxys belonging to four species were captured with an apparent density of 136.78 flies per trap and day (f/t/d). Twelve species of tabanids were collected with 3524 individuals with an apparent density of 10.87 f/t/d. Stomoxys niger niger was the most abundant species while for tabanids, Tabanus taeniola (48.63%) was most represented. In the savanna, 4501 (13.89 f/t/d) Stomoxys were collected and regrouped under five species with S. n. niger (70.91%) highly represented and 547 (1.68 f/t/d) tabanids collected and regrouped under 10 species with Tabanus taeniola as the most dominant species. Trap apparent density (ADT) varied with sampled sites, seasons and the prevailing weather conditions in the forest and savanna.Conclusion: From the present finding, it can be deduced that T. taeniola and S. n. niger were the most abundant and indicate the risk of mechanical transmission of dangerous pathogens such as bovine trypanosomosis.
Pulmonary B Lymphocytes Ameliorate Alzheimer's Disease-Like Neuropathology
Authors: Feng Weixi , Zhang Yanli , Wang Tianqi , Li Qian , Wang Ze , Zou Ying , Sheng Chengyu , Chen Yan , Pang Yingting , Ding Fengfei , Marshall Charles , Shi Jingping , Nedergaard Maiken , Xiao Ming 
Published: July 2021

Abstract
Increasing evidences reveal that the peripheral immune system is involved in the pathogenesis of Alzheimer's disease (AD). Here, we report that pulmonary B lymphocytes mitigate beta-Amyloid (Aβ) pathology in 5xFAD mice. The proportion of B cells, rather than T cells, increases within the brain, meningeal and lung tissues in 3-month-old 5xFAD mice. Deletion of mature B cells aggravates Aβ load and memory deficits of 5xFAD mice. Mechanistically, pulmonary B cells can migrate to the brain parenchyma and produce interleukin-35, which inhibits neuronal β-site APP-cleaving enzyme 1 expression, and subsequently reduces the production of Aβ. In turn, pulmonary B cell proliferation is associated with activation of the toll-like receptor/nuclear factor kappa-B pathway through elevated Aβ that is drained from the brain parenchyma to the lungs via meningeal lymphatics. Furthermore, promoting pulmonary B cell proliferation via overexpression of B-cell-activating factor ameliorates brain Aβ load and improves cognitive functions of 10-month-old 5xFAD mice. Together, these results highlight the lungs as both immune targets and effector organs in Aβ pathogenesis. Pulmonary B cells could serve as a potential target against AD.
Laryngeal mask airway versus endotracheal tube for airway management during laparoscopic percutaneous extraperitoneal closure for inguinal hernia in pediatric day surgery: a retrospective analysis
Authors: Iwade Tamaki , Ohno Koichi 
Published: July 2021

Abstract
BackgroundAlthough endotracheal tube is preferred for airway management during laparoscopic percutaneous extraperitoneal closure for inguinal hernias, laryngeal mask airway may also be used. However, few studies have reported the usefulness of laryngeal mask airway during laparoscopic percutaneous extraperitoneal closure. Our study aims to report the advantages of laryngeal mask airway versus endotracheal tube during laparoscopic percutaneous extraperitoneal closure for inguinal hernia in pediatric day surgery.MethodsThe records of 56 patients (Group I, endotracheal tube; Group II, laryngeal mask airway) treated for inguinal hernia using laparoscopic percutaneous extraperitoneal closure between November 2018 and December 2019 were retrospectively reviewed. The duration of anesthesia; changes in hemodynamics (heart rate and systolic/diastolic blood pressure), end-tidal carbon dioxide, and bispectral index; and postoperative complications were analyzed.ResultsGroups I and II had 39 and 17 patients, respectively. The duration of anesthesia and surgery and changes in hemodynamics and bispectral index were similar between the two groups. Induction and recovery times were significantly shorter and changes in end-tidal carbon dioxide were more significant in Group II (p < 0.05). The incidence of sore throat and nausea was higher in Group I (p < 0.05).ConclusionsLaryngeal mask airway was equivalent to endotracheal tube in terms of performance during laparoscopic percutaneous extraperitoneal closure, although induction and recovery were achieved sooner in laryngeal mask airway, with a lower incidence of sore throat and nausea.Trial RegistrationNot applicable
Atypical Renal Cyst (Clear Cell Type)
Authors: Li Yu-Ying , Sun Ming-Fang , Yuan Ning-Lang , Liu Yang , Zhang Di 
Published: July 2021

Abstract
Background: Atypical cysts do not diagnostic criteria form classification as any of the variants of renal tumors, which have led to diagnostic difficulties. When these cysts have been encountered in surgical pathology practice it can be easily misdiagnosed as renal carcinoma and results in overtreatment. Case presentation: A 55-year-old Chinese woman with a right renal cystic mass came to our hospital. The computed tomography revealed bilateral cysts in both kidneys. In histology, the right renal cystic mass was arranged by stratified epithelium with short papillary projections and tufting. The covered epithelial cells were characterized by clear cytoplasm and small nuclei. Immunohistochemistry, these epithelial cells were diffusely positive for CK7, CK8, CK18, Vimentin and EMA, weakly positive for RCC and PAX-8, while diffusely negative for AMACR, CD10 and TFE-3. And the Ki-67 positive rate was 3%. Based on the immunohistochemical profile and histological features, the final diagnosis is an atypical renal cyst (clear cell type). The patient underwent right nephrectomy. The patient is alive and without any sign of subsequent renal cancer during 11 months of follow-up. Conclusion: Atypical renal cyst is extremely rare among the routine renal pathological diagnosis, we report this case to enhance the understanding and differential diagnosis of the atypical renal cyst and remind the pathologist of this precancerous lesion, and therefore, to avoid unnecessary or excessive treatment.
Factors Associated with Depression and Anxiety in the Adult Population of Qatar in the First Year of the COVID-19 pandemic: Results from an Online Survey
Authors: Khaled Salma M. , Amro Iman , Bader Lina , Woodruff Peter , Alabdulla Majid A , Bellaj Tarek , Marzouki Youssri , Hasan Yousef , Al-Kaabi Ibrahim , Haddad Peter M. 
Published: July 2021

Abstract

Background: There is limited data from Arabic-speaking countries on risk factors for depression and anxiety during the COVID-19 pandemic. Country-specific data is necessary given differences in culture, demographics, COVID-19 infection and mortality rates.Aim: To identify factors associated with symptoms of depression-anxiety in the adult population of Qatar during the first year of the COVID-19 pandemic.Method: We conducted a cross-sectional online survey in Qatar between July and December 2020 after the first COVID-19 wave and before the beginning of the second wave. Depression-anxiety was defined as a cut-off of 20 or higher on the PHQ-ADS scale. Results: Of 1138 participants, 71.05% were female, 69.0% Arabs, and 70.0% Non-Qataris. 77% were < 40 years (the median age in Qatar is 32 years). In a fully-adjusted model, six variables were significantly associated with PHQ-ADS; Arab ethnicity (OR=1.67, p=0.026), never married (OR=2.04, p < 0.001 (versus married), prior history of psychiatric disorder (versus no history) (OR=1.76, p=0.039), increased worries due to social media use for COVID-related news/updates (OR=1.72, p=0.003), those with a history of COVID-19 (OR=1.76, p=0.039), loneliness (OR=1.91, p < 0.001), and lower levels of religiosity (OR=0.96, p=0.039). These associations also pertained in the reduced model, with exception of religiosity which was only marginally statistically significant (OR=0.97, p=0.055).Conclusions: The potential risk factors identified may assist with anxiety and depression prevention in future COVID-19 waves, and similar crises, and assist with early intervention to treat sufferers.