bluuebunny/crossref_metadata_2025_split huggingface dataset:
Phenotypic diversity of genetic Creutzfeldt–Jakob disease: a histo-molecular-based classification
Authors: Baiardi Simone , Rossi Marcello , Mammana Angela , Appleby Brian S. , Barria Marcelo A. , Calì Ignazio , Gambetti Pierluigi , Gelpi Ellen , Giese Armin , Ghetti Bernardino , Herms Jochen , Ladogana Anna , Mikol Jacqueline , Pal Suvankar , Ritchie Diane L. , Ruf Viktoria , Windl Otto , Capellari Sabina , Parchi Piero 
Published: July 2021

AbstractThe current classification of sporadic Creutzfeldt–Jakob disease (sCJD) includes six major clinicopathological subtypes defined by the physicochemical properties of the protease-resistant core of the pathologic prion protein (PrPSc), defining two major PrPSc types (i.e., 1 and 2), and the methionine (M)/valine (V) polymorphic codon 129 of the prion protein gene (PRNP). How these sCJD subtypes relate to the well-documented phenotypic heterogeneity of genetic CJD (gCJD) is not fully understood. We analyzed molecular and phenotypic features in 208 individuals affected by gCJD, carrying 17 different mutations, and compared them with those of a large series of sCJD cases. We identified six major groups of gCJD based on the combination PrPSc type and codon 129 genotype on PRNP mutated allele, each showing distinctive histopathological characteristics, irrespectively of the PRNP associated mutation. Five gCJD groups, named M1, M2C, M2T, V1, and V2, largely reproduced those previously described in sCJD subtypes. The sixth group shared phenotypic traits with the V2 group and was only detected in patients carrying the E200K-129M haplotype in association with a PrPSc type of intermediate size (“i”) between type 1 and type 2. Additional mutation-specific effects involved the pattern of PrP deposition (e.g., a “thickened” synaptic pattern in E200K carriers, cerebellar “stripe-like linear granular deposits” in those with insertion mutations, and intraneuronal globular dots in E200K-V2 or -M”i”). A few isolated cases linked to rare PRNP haplotypes (e.g., T183A-129M), showed atypical phenotypic features, which prevented their classification into the six major groups. The phenotypic variability of gCJD is mostly consistent with that previously found in sCJD. As in sCJD, the codon 129 genotype and physicochemical properties of PrPSc significantly correlated with the phenotypic variability of gCJD. The most common mutations linked to CJD appear to have a variable and overall less significant effect on the disease phenotype, but they significantly influence disease susceptibility often in a strain-specific manner. The criteria currently used for sCJD subtypes can be expanded and adapted to gCJD to provide an updated classification of the disease with a molecular basis.
The evolving epidemiology of acute gastroenteritis in hospitalized children in Italy
Authors: Stanyevic Brigida , Sepich Margherita , Biondi Samanta , Baroncelli Giampiero Igli , Peroni Diego , Di Cicco Maria 
Published: July 2021

AbstractFew data are available on the prevalence and features of acute gastroenteritis (AGE) in hospitalized children in Italy, where specific rotavirus vaccines were introduced into the national vaccination plan in 2017. To evaluate vaccination effects on AGE epidemiology, we analysed data from children aged ≤ 18 years admitted for AGE at the University Hospital of Pisa in 2019, comparing them with those recorded in 2012. Demographical, clinical, diagnostic, and treatment data were collected reviewing medical records and were therefore compared. In 2019 and 2012, 86 (median age 2.5 years [IQR 1.4–5.9]) and 85 children (median age 2.3 years [IQR 1.3–5.1]) were respectively admitted with AGE. The most common symptoms were diarrhoea and vomiting; decreased skin turgor was more frequent in 2019 (54% and 34% respectively, p = 0.01). Viral infections were more common than bacterial ones; in 2019, a decrease in rotavirus infections (67% and 22%, p = 0.003) and an increase in adenovirus infections (50% and 10%, p = 0.002) and in the number of patients with negative stool testing (58% and 39%, p = 0.04) were found.Conclusions: Viral infections are the leading cause of AGE in hospitalized children in Italy. The introduction of rotavirus vaccines did not reduce the number of hospitalizations per year. Adenovirus and other non-routinely screened viruses may be undergoing a selection process making them common causative agents for AGE.

What is Known:• Rotavirus is the leading cause of acute severe gastroenteritis in children worldwide, especially < 5 years of age.• The introduction of specific vaccines may be changing its epidemiology.• Few data are available on acute gastroenteritis in hospitalized children in Italy.

What is New:• Viral infections are the leading cause of acute gastroenteritis in hospitalized children in Italy.• Specific vaccines are reducing rotavirus infections, but adenovirus and other non-routinely screened viruses may be undergoing a selection process making them common causative agents for gastroenteritis.
„Jetzt Sprichst Du!“
Authors: Schabus Manuel , Eigl Esther-Sevil 
Published: July 2021

ZusammenfassungDie Umfrage „Jetzt Sprichst Du!“ veranschaulicht eindrücklich die psychosozialen Belastungen und die Beeinträchtigung von Kindern und Jugendlichen in Österreich während der aktuellen Coronapandemie. Im Rahmen einer Online-Umfrage wurden 5483 Kinder und Jugendliche im Alter von 6 bis 18 Jahren bezüglich ihrer Gefühle, Ängste, Sorgen und Einschätzungen im Zusammenhang mit der Coronapandemie befragt. Es zeigt sich, dass die Kinder und Jugendlichen durch die Situation geängstigt sind und Mädchen hierbei über alle Altersgruppen hinweg stärker belastet sind. In diesem Zusammenhang wird auch deutlich, dass das Risiko einer COVID-19-assoziierten Hospitalisierung ähnlich wie bei Erwachsenen von den Kindern wie Jugendlichen massiv überschätzt wird. Auch macht sich in allen Altersgruppen eine hohe Perspektivenlosigkeit aufgrund der anhaltend herausfordernden Situation bemerkbar. Ferner häufen sich Gefühle von Wut, Ärger, Einsamkeit und Traurigkeit und es zeigt sich eine alarmierende Verschlechterung der Schlafqualität und eine Zunahme der Schlafproblematiken. Die Daten der Umfrage „Jetzt Sprichst Du!“ betonen die Notwendigkeit eines unabdingbaren und raschen Handelns, um sowohl die psychosozialen, entwicklungspsychologischen als auch gesundheitlichen Kollateralschäden in dieser jungen Altersgruppe einzudämmen, soweit dies heute noch möglich ist.
Integrating Aquatic Metabolism and Net Ecosystem CO2 Balance in Short- and Long-Hydroperiod Subtropical Freshwater Wetlands
Authors: Malone Sparkle L. , Zhao Junbin , Kominoski John S. , Starr Gregory , Staudhammer Christina L. , Olivas Paulo C. , Cummings Justin C. , Oberbauer Steven F. 
Published: July 2021

AbstractHow aquatic primary productivity influences the carbon (C) sequestering capacity of wetlands is uncertain. We evaluated the magnitude and variability in aquatic C dynamics and compared them to net ecosystem CO2 exchange (NEE) and ecosystem respiration (Reco) rates within calcareous freshwater wetlands in Everglades National Park. We continuously recorded 30-min measurements of dissolved oxygen (DO), water level, water temperature (Twater), and photosynthetically active radiation (PAR). These measurements were coupled with ecosystem CO2 fluxes over 5 years (2012–2016) in a long-hydroperiod peat-rich, freshwater marsh and a short-hydroperiod, freshwater marl prairie. Daily net aquatic primary productivity (NAPP) rates indicated both wetlands were generally net heterotrophic. Gross aquatic primary productivity (GAPP) ranged from 0 to − 6.3 g C m−2 day−1 and aquatic respiration (RAq) from 0 to 6.13 g C m−2 day−1. Nonlinear interactions between water level, Twater, and GAPP and RAq resulted in high variability in NAPP that contributed to NEE. Net aquatic primary productivity accounted for 4–5% of the deviance explained in NEE rates. With respect to the flux magnitude, daily NAPP was a greater proportion of daily NEE at the long-hydroperiod site (mean = 95%) compared to the short-hydroperiod site (mean = 64%). Although we have confirmed the significant contribution of NAPP to NEE in both long- and short-hydroperiod freshwater wetlands, the decoupling of the aquatic and ecosystem fluxes could largely depend on emergent vegetation, the carbonate cycle, and the lateral C flux.
Unveiling endogeneity and temporal dependence in energy prices and demand in Iberian countries: a stochastic hidden Markov model approach
Authors: Antunes Jorge , Gil-Alana Luis Alberiko , Riccardi Rossana , Tan Yong , Wanke Peter 
Published: July 2021

AbstractIn this paper, we analyze the temporal dependence in energy prices and demand using daily data of Portugal and Spain over the period 2007–2017. The methodology used is based on a stochastic Hidden Markov Model and the results indicate first that all significant relationships between energy prices and demands were found to be positive; second, spot prices are only time dependent on future prices and spot energy, while future energy is solely time dependent on spot energy behavior; third, future prices are not only autocorrelated but also time-dependent with spot energy and future energy demands level; and finally, spot energy is autocorrelated and time-dependent with future prices and future energy. Policy implications of the results obtained are presented at the end of the article.
We do not measure what we aim to measure: Testing Three Measurement Models for Nationalism and Patriotism
Authors: Mußotter Marlene 
Published: July 2021

AbstractThe measurement models for both nationalism and patriotism originally developed by Blank and Schmidt are broadly established. Despite their widespread usage in Germany and beyond, concerns have been voiced about the operationalisation of these nation-related concepts. However, in previous scholarship little attention has been devoted to systematically reviewing the models’ validity. This paper’s major goal is to contribute substantially to research on operationalising national attitudes by thoroughly examining how both nationalism and patriotism are measured and how valid the predominant measurement models really are. By running a confirmatory factor analysis, three measurement models based on the ISSP data of 2003 are replicated and empirically reviewed. By conducting a single-country analysis, the models are tested for the German case, including the evaluation of measurement invariance for both Eastern and Western Germany. Although the selected measurement models yield satisfying results, the paper identifies considerable shortcomings with regard to the way both nationalism and patriotism are empirically approached. It calls for a reconceptualising of the prevailing concept of pride and thus challenges the predominant operationalisation.
Between mechanical clocks and emergent flocks: complexities in biology
Authors: Gross Fridolin 
Published: July 2021

AbstractEven though complexity is a concept that is ubiquitously used by biologists and philosophers of biology, it is rarely made precise. I argue that a clarification of the concept is neither trivial nor unachievable, and I propose a unifying framework based on the technical notion of “effective complexity” that allows me to do justice to conflicting intuitions about biological complexity, while taking into account several distinctions in the usage of the concept that are often overlooked. In particular, I propose a distinction between two kinds of complexity, “mechanical” and “emergent”, which can be understood as different ways of relating the effective complexity of mechanisms and of behaviors in biological explanations. I illustrate the adequacy of this framework by discussing different attempts to understand intracellular organization in terms of pathways and networks. My framework provides a different way of thinking about recent philosophical debates, for example, on the difference between mechanistic and topological explanations and about the concept of emergence. Moreover, it can contribute to a proper assessment of metascientific arguments that invoke biological complexity.
A Spectroscopic Paradox: The Interaction of Methanol with ZSM-5 at Room Temperature
Authors: Zachariou Andrea , Hawkins Alexander P. , Howe Russell F. , Barrow Nathan , Bradley Jonathan , Collier Paul , Lennon David , Parker Stewart F. 
Published: July 2021

AbstractThe adsorption of methanol in HZSM-5 at low temperatures has long been regarded as an associative process involving hydrogen bonding to the acidic zeolite hydroxyl groups. Recent studies employing inelastic neutron scattering spectroscopy (INS) have reported that complete dissociation to methoxylate the zeolite occurs at 298 K, and infrared evidence for a partial dissociation at 298 K has also been described. Here we investigate the apparent contradictions between different techniques, using a combination of INS, infrared spectroscopy and solid-state NMR spectroscopy, including isotopic substitution experiments. Different possible explanations are proposed and considered; we conclude that at room temperature methanol is very largely associatively adsorbed, although the presence of some small extent (>1%) of methoxylation cannot be ruled out.
Occurrence and size distribution of silver nanoparticles in wastewater effluents from various treatment processes in Canada
Authors: Gagnon Christian , Turcotte Patrice , Gagné François , Smyth Shirley Anne 
Published: July 2021

AbstractThe occurrence of silver (Ag) in urban effluents is partly associated with the increasing use of silver nanoparticles (Ag NPs) as an antiseptic agent in various consumer products. Distinction among Ag forms must be taken into account in the assessment of exposure and toxicological effects to aquatic organisms. Wastewater treatment processes effectively remove Ag particles and colloids (mostly > 95%), but this still leaves notable concentrations (in order of ng/L) escaping to effluent-receiving waters. Total suspended Ag concentrations in various studied effluents ranged from 0.1 to 6 ng/L. The purpose of this study was then to measure and characterize Ag NPs in urban effluents for their concentrations and size distribution using the single particle ICP-MS technique (SP-ICP-MS). Wastewater influents and effluents from various treatment plants—from aerated lagoons to advanced treatment technology—were collected for three sampling days. Our results showed the presence of Ag NP in all samples with concentrations reaching 0.5 ng/L on a mass basis. However, on a particle number basis, Ag NP concentrations (expressed in particle/mL) in the 20–34-nm fraction (up to 3400 particles/mL) were much more abundant (> 700%) than in the > 35-nm larger fraction. The proportion of Ag at the nanoscale (1–100 nm) represents less than 8% of the total suspended Ag for all effluent samples, regardless of their origins. A significant correlation (linear regression: r2 > 0.7) was observed between Ag NP and total suspended Ag concentrations in investigated effluents. Because Ag nanotoxicity is size dependent, the determination of size distribution and exposure concentration on a particle number basis is urgently needed for risk assessment of this class of nanoparticles.
Lipid and metabolite correlation networks specific to clinical and biochemical covariate show differences associated with sexual dimorphism in a cohort of nonagenarians
Authors: Di Cesare Francesca , Tenori Leonardo , Meoni Gaia , Gori Anna Maria , Marcucci Rossella , Giusti Betti , Molino-Lova Raffaele , Macchi Claudio , Pancani Silvia , Luchinat Claudio , Saccenti Edoardo 
Published: July 2021

AbstractThis study defines and estimates the metabolite-lipidic component association networks constructed from an array of 20 metabolites and 114 lipids identified and quantified via NMR spectroscopy in the serum of a cohort of 355 Italian nonagenarians and ultra-nonagenarian. Metabolite-lipid association networks were built for men and women and related to an array of 101 clinical and biochemical parameters, including the presence of diseases, bio-humoral parameters, familiarity diseases, drugs treatments, and risk factors. Different connectivity patterns were observed in lipids, branched chains amino acids, alanine, and ketone bodies, suggesting their association with the sex-related and sex-clinical condition-related intrinsic metabolic changes. Furthermore, our results demonstrate, using a holistic system biology approach, that the characterization of metabolic structures and their dynamic inter-connections is a promising tool to shed light on the dimorphic pathophysiological mechanisms of aging at the molecular level.
Waning antibody responses in COVID-19: what can we learn from the analysis of other coronaviruses?
Authors: Hamady Ali , Lee JinJu , Loboda Zuzanna A. 
Published: July 2021

Abstract
Objectives
The coronavirus disease 2019 (COVID-19), caused by the novel betacoronavirus severe acute respiratory syndrome 2 (SARS-CoV-2), was declared a pandemic in March 2020. Due to the continuing surge in incidence and mortality globally, determining whether protective, long-term immunity develops after initial infection or vaccination has become critical.

Methods/Results
In this narrative review, we evaluate the latest understanding of antibody-mediated immunity to SARS-CoV-2 and to other coronaviruses (SARS-CoV, Middle East respiratory syndrome coronavirus and the four endemic human coronaviruses) in order to predict the consequences of antibody waning on long-term immunity against SARS-CoV-2. We summarise their antibody dynamics, including the potential effects of cross-reactivity and antibody waning on vaccination and other public health strategies. At present, based on our comparison with other coronaviruses we estimate that natural antibody-mediated protection for SARS-CoV-2 is likely to last for 1–2 years and therefore, if vaccine-induced antibodies follow a similar course, booster doses may be required. However, other factors such as memory B- and T-cells and new viral strains will also affect the duration of both natural and vaccine-mediated immunity.

Conclusion
Overall, antibody titres required for protection are yet to be established and inaccuracies of serological methods may be affecting this. We expect that with standardisation of serological testing and studies with longer follow-up, the implications of antibody waning will become clearer.
An improved generative adversarial network with modified loss function for crack detection in electromagnetic nondestructive testing
Authors: Tian Lulu , Wang Zidong , Liu Weibo , Cheng Yuhua , Alsaadi Fuad E. , Liu Xiaohui 
Published: July 2021

AbstractIn this paper, an improved generative adversarial network (GAN) is proposed for the crack detection problem in electromagnetic nondestructive testing (NDT). To enhance the contrast ratio of the generated image, two additional regulation terms are introduced in the loss function of the underlying GAN. By applying an appropriate threshold to the segmentation of the generated image, the real crack areas and the fake crack areas (which are affected by the noises) are accurately distinguished. Experiments are carried out to show the superiority of the improved GAN over the original one on crack detection tasks, where a real-world NDT dataset is exploited that consists of magnetic optical images obtained using the electromagnetic NDT technique.
The Mechanism of Metallosis After Total Hip Arthroplasty
Authors: Ude Chinedu C. , Esdaille Caldon J. , Ogueri Kenneth S. , Kan Ho-Man , Laurencin Samuel J. , Nair Lakshmi S. , Laurencin Cato T. 
Published: July 2021

AbstractMetallosis is defined as the accumulation and deposition of metallic particles secondary to abnormal wear from prosthetic implants that may be visualized as abnormal macroscopic staining of periprosthetic soft tissues. This phenomenon occurs secondary to the release of metal ions and particles from metal-on-metal hip implants in patients with end-stage osteoarthritis. Ions and particles shed from implants can lead to local inflammation of surrounding tissue and less commonly, very rare systemic manifestations may occur in various organ systems. With the incidence of total hip arthroplasty increasing as well as rates of revisions due to prosthesis failure from previous metal-on-metal implants, metallosis has become an important area of research. Bodily fluids are electrochemically active and react with biomedical implants. Particles, especially cobalt and chromium, are released from implants as they abrade against one another into the surrounding tissues. The body’s normal defense mechanism becomes activated, which can elicit a cascade of events, leading to inflammation of the immediate surrounding tissues and eventually implant failure. In this review, various mechanisms of metallosis are explored. Focus was placed on the atomic and molecular makeup of medical implants, the component/surgical associated factors, cellular responses, wear, tribocorrosion, joint loading, and fluid pressure associated with implantation. Current treatment guidelines for failed implants include revision surgery. An alternative treatment could be chelation therapy, which may drive future studies.Lay SummaryArthroplasty is an invasive procedure which disrupts surrounding joint tissues, and can greatly perturb the joint’s immune homeostasis. In some instances, this may pose a difficult challenge to implant integration. Particles released from implants into the surrounding joint tissues activate the body’s defense mechanism, eliciting a cascade of events, which leads to biotribocorrosion and electrochemical attacks on the implant. This process may lead to the release of even more particles. Besides, implant makeup and designs, frictions between bearing surfaces, corrosion of non-moving parts with modular junctions, surgical mistakes, patient factor, comorbidities, and loosened components can alter the expected function of implants. High accumulations of these ions and particulates result in metallosis, with accompanying adverse complications. Current recommended treatment for failed prosthesis is revision surgeries. However, chelation therapy as a prophylactic intervention may be useful in future efforts but more investigation is required.
Scolicidal activity of biosynthesized zinc oxide nanoparticles by Mentha longifolia L. leaves against Echinococcus granulosus protoscolices
Authors: Shnawa Bushra H. , Hamad Samir M. , Barzinjy Azeez A. , Kareem Payman A. , Ahmed Mukhtar H. 
Published: July 2021

AbstractCystic echinococcosis is a public health problem in developing countries that practice sheep breeding extensively. In the current study, the protoscolicidal activity of biosynthesized zinc oxide nanoparticles (ZnO NPs) derived from Mentha longifolia L. leaf extracts was investigated. The resultant ZnO NPs were characterized by means of various analytical techniques, such as ultraviolet–visible (UV–Vis) spectrometry, Fourier transform infrared (FT-IR) spectrophotometry, X-ray diffraction (XRD), scanning electron microscopy (SEM), and energy-dispersive X-ray spectroscopy (EDX) analysis. The results showed that the ZnO NP had the highest scolicidal activity at 400 ppm concentration after 150 min of exposure time, showing 100% mortality rate. The treated protoscolices exhibited loss of viability with several morphological alterations. Hence, an easy and effective green synthesis of ZnO NPs, with efficient scolicidal potential, is reported in this study.