bluuebunny/crossref_metadata_2025_split huggingface dataset:
Consequences of PDGFRα+fibroblast reduction in adult murine hearts
Authors: Kuwabara Jill T. , Bhutada Sumit , Shettigar Vikram , Gojanovich Greg S. , DeAngelo Lydia P. , Heckl Jack R. , Jahansooz Julia R. , Tacdol Dillon K. , Ziolo Mark T. , Apte Suneel S. , Tallquist Michelle D. 
Published: May 2021

AbstractFibroblasts produce the majority of collagen in the heart and are thought to regulate extracellular matrix (ECM) turnover. Although fibrosis accompanies many cardiac pathologies and is generally deleterious, the role of fibroblasts in maintaining the basal ECM network and in fibrosisin vivois poorly understood. We genetically ablated fibroblasts in mice to evaluate the impact on homeostasis of adult ECM and cardiac function after injury. Fibroblast-ablated mice demonstrated a 60-80% reduction in cardiac fibroblasts, which did not overtly alter fibrillar collagen or the ECM proteome evaluated by quantitative mass spectrometry and N-terminomics. However, the distribution and quantity of collagen VI, a microfibrillar collagen that forms an open network with the basement membrane, was altered. In fibroblast-ablated mice, cardiac function was better preserved following angiotensin II/phenylephrine (AngII/PE)-induced fibrosis and myocardial infarction. Analysis of cardiomyocyte function demonstrated weaker contractions and slowed calcium decline in both uninjured and AngII/PE infused fibroblast-ablated mice. Moreover, fibroblast-ablated hearts had a similar gene expression profile to hearts with physiological hypertrophy after AngII/PE infusion. Our results indicate that the adult mouse heart tolerated a significant degree of fibroblast loss with potential beneficial impacts on cardiac function. Controlled fibroblast reduction may have therapeutic value in heart disease by providing cardioprotective effects.
A bioorthogonal chemical reporter for fatty acid synthase-dependent protein acylation
Authors: Karthigeyan Krithika P. , Zhang Lizhi , Loiselle David R. , Haystead Timothy A. J. , Bhat Menakshi , Yount Jacob S. , Kwiek Jesse J. 
Published: May 2021

SummaryCells acquire fatty acids from dietary sources or viade novopalmitate production by fatty acid synthase (FASN). Although most cells express FASN at low levels, it is upregulated in cancers and during replication of many viruses. The precise role of FASN in disease pathogenesis is poorly understood, and whetherde novofatty acid synthesis contributes to host or viral protein acylation has been traditionally difficult to study. We describe a cell permeable, click-chemistry compatible alkynyl-acetate analog (Alk-4) that functions as a reporter of FASN-dependent protein acylation. In a FASN-dependent manner, Alk-4 selectively labeled the cellular protein interferon-induced transmembrane protein 3 (IFITM3) at its palmitoylation sites, and the HIV-1 matrix protein at its myristoylation site. Alk-4 metabolic labeling also enabled biotin-based purification and identification of more than 200 FASN-dependent acylated cellular proteins. Thus, Alk-4 is a useful bioorthogonal tool to selectively probe FASN-mediated protein acylation in normal and diseased states.
The VE-cadherin/AmotL2 mechanosensory pathway suppresses aortic inflammation and the formation of abdominal aortic aneurysms
Authors: Zhang Yuanyuan , Hutterer Evelyn , Hultin Sara , Bergman Otto , Forteza Maria J. , Andonovic Zorana , Ketelhuth Daniel F.J. , Roy Joy , Eriksson Per , Holmgren Lars 
Published: May 2021

AbstractArterial endothelial cells (ECs) have the ability to respond to mechanical forces exerted by fluid shear stress. This response is of importance, as it is protective against vascular diseases such as atherosclerosis and aortic aneurysms. Mechanical forces are transmitted at the sites of adhesion to the basal membrane as well as cell-cell junctions where protein complexes connect to the cellular cytoskeleton to relay force into the cell. Here we present a novel protein complex that connects junctional VE-cadherin and radial actin filaments to the LINC complex in the nuclear membrane. We show that the scaffold protein AmotL2 is essential for the formation of radial actin filaments and the flow-induced alignment of aortic and arterial ECs. The deletion of endothelial AmotL2 alters nuclear shape as well as subcellular positioning. Molecular analysis shows that VE-cadherin is mechanically associated with the nuclear membrane via binding to AmotL2 and Actin. Furthermore, the deletion of AmotL2 in ECs provokes a pro-inflammatory response and abdominal aortic aneurysms (AAA) in the aorta of mice on a normal diet. Remarkably, transcriptome analysis of AAA samples from human patients revealed a negative correlation between AmotL2 expression and aneurysm diameters, as well as a positive correlation between AmotL2 and YAP expression. These findings provide a conceptual framework regarding how mechanotransduction in the junctions is coupled with vascular disease.
Symmetry breaking in the female germline cyst
Authors: Nashchekin D. , Busby L. , Jakobs M. , Squires I. , St Johnston D. 
Published: May 2021

AbstractIn mammals and flies, only a limited number of cells in a multicellular female germline cyst become oocytes, but how the oocyte is selected is unknown. Here we show that the microtubule minus end-stabilizing protein, Patronin/CAMSAP marks the futureDrosophilaoocyte and is required for oocyte specification. The spectraplakin, Shot, recruits Patronin to the fusome, a branched structure extending into all cyst cells. Patronin stabilizes more microtubules in the cell with most fusome and this weak asymmetry is amplified by Dynein-dependent transport of Patronin-stabilized microtubules. This forms a polarized microtubule network, along which Dynein transports oocyte determinants into the presumptive oocyte. Thus, Patronin amplifies a weak fusome anisotropy to break cyst symmetry. These findings reveal a molecular mechanism of oocyte selection in the germline cyst.
The Slit-binding Ig1 domain is required for multiple axon guidance activities ofDrosophilaRobo2
Authors: Howard LaFreda J. , Reichert Marie C. , Evans Timothy A. 
Published: May 2021

AbstractDrosophilaRobo2 is a member of the evolutionarily conserved Roundabout (Robo) family of axon guidance receptors. The canonical role of Robo receptors is to signal midline repulsion in response to their cognate Slit ligands, which bind to the N-terminal Ig1 domain in most Robo family members. In theDrosophilaembryonic ventral nerve cord, Robo1 and Robo2 cooperate to signal Slit-dependent midline repulsion, while Robo2 also regulates the medial-lateral position of longitudinal axon pathways and acts non-autonomously to promote midline crossing of commissural axons. Although it is clear that Robo2 signals midline repulsion in response to Slit, it is less clear whether Robo2’s other activities are also Slit-dependent. To determine which of Robo2’s axon guidance roles depend on its Slit-binding Ig1 domain, we have used a CRISPR/Cas9-based strategy replace the endogenousrobo2gene with arobo2variant from which the Ig1 domain has been deleted(robo2ΔIg1). We compare the expression and localization of Robo2ΔIg1 protein with that of full-length Robo2 in embryonic neuronsin vivo, and examine its ability to substitute for Robo2 to mediate midline repulsion and lateral axon pathway formation. We find that removal of the Ig1 domain from Robo2ΔIg1 disrupts both of these axon guidance activities. In addition, we find that the Ig1 domain of Robo2 is required for its proper subcellular localization in embryonic neurons, a role that is not shared by the Ig1 domain of Robo1. Finally, we report that although FasII-positive lateral axons are misguided in embryos expressing Robo2ΔIg1, the axons that normally express Robo2 are correctly guided to the lateral zone, suggesting that Robo2 may guide lateral longitudinal axons through a cell non-autonomous mechanism.
A Mean Shift Algorithm for Drift Correction in Localization Microscopy
Authors: Fazekas Frank J , Shaw Thomas R , Kim Sumin , Bogucki Ryan A , Veatch Sarah L 
Published: May 2021

AbstractSingle molecule localization microscopy (SMLM) techniques transcend the diffraction limit of visible light by localizing isolated emitters sampled stochastically. This time-lapse imaging necessitates long acquisition times, over which sample drift can become large relative to the localization precision. Here we present a novel, efficient, and robust method for estimating drift using a simple peak-finding algorithm based on mean shifts that is effective for SMLM in 2 or 3 dimensions.
Numerical and experimental research on the erosion of solid-liquid two-phase flow in transport butterfly valve based on DEM method
Authors: Xu Benliang , Zhu Zuchao , Lin Zhe , Wang Dongrui , Ma Guangfei 
Published: May 2021

Purpose
The purpose of this paper is to analyze the mechanism of particle erosion in butterfly valve pipelines under hydraulic transportation conditions. The results will affect the sealing and safety of butterfly valve pipelines and hopefully serve as reference for the anti-erosion design of butterfly valve pipelines.

Design/methodology/approach
Through the discrete element method (DEM) simulation that considers the force between particles, the detached eddy simulation (DES) turbulence model based on realizable k-epsilon is used to simulate the solid-liquid two-phase flow-induced erosion condition when the butterfly valve is fully opened. The simulation is verified by building an experimental system correctness. The solid-liquid two-phase flow characteristics, particle distribution and erosion characteristics of the butterfly valve pipeline under transportation conditions are studied.

Findings
The addition of particles may enhance the high-speed area behind the valve. It first increases and then decreases with increasing particle size. With increasing particle size, the low-velocity particles change from being uniformly distributed in flow channel to first gathering in the front of the valve and, then, to gathering in lower part of it. Fluid stagnation at the left arc-shaped flange leads to the appearance of two high-speed belts in the channel. With increasing fluid velocity, high-speed belts gradually cover the entire valve surface by focusing on the upper and lower ends, resulting in the overall aggravation of erosion.

Originality/value
Considering the complexity of solid-liquid two-phase flow, this is the first time that the DEM method with added inter-particle forces and the DES turbulence model based on realizable k-epsilon has been used to study the flow characteristics and erosion mechanism of butterfly valves under fully open transportation conditions.
Analysis of the effects of spatiotemporal demand data aggregation methods on distance and volume errors
Authors: Hornberger Zachary , Cox Bruce , Hill Raymond R. 
Published: May 2021

PurposeLarge/stochastic spatiotemporal demand data sets can prove intractable for location optimization problems, motivating the need for aggregation. However, demand aggregation induces errors. Significant theoretical research has been performed related to the modifiable areal unit problem and the zone definition problem. Minimal research has been accomplished related to the specific issues inherent to spatiotemporal demand data, such as search and rescue (SAR) data. This study provides a quantitative comparison of various aggregation methodologies and their relation to distance and volume based aggregation errors.Design/methodology/approachThis paper introduces and applies a framework for comparing both deterministic and stochastic aggregation methods using distance- and volume-based aggregation error metrics. This paper additionally applies weighted versions of these metrics to account for the reality that demand events are nonhomogeneous. These metrics are applied to a large, highly variable, spatiotemporal demand data set of SAR events in the Pacific Ocean. Comparisons using these metrics are conducted between six quadrat aggregations of varying scales and two zonal distribution models using hierarchical clustering.FindingsAs quadrat fidelity increases the distance-based aggregation error decreases, while the two deliberate zonal approaches further reduce this error while using fewer zones. However, the higher fidelity aggregations detrimentally affect volume error. Additionally, by splitting the SAR data set into training and test sets this paper shows the stochastic zonal distribution aggregation method is effective at simulating actual future demands.Originality/valueThis study indicates no singular best aggregation method exists, by quantifying trade-offs in aggregation-induced errors practitioners can utilize the method that minimizes errors most relevant to their study. Study also quantifies the ability of a stochastic zonal distribution method to effectively simulate future demand data.
Banks’ international assets and sovereign default risk
Authors: Gori Filippo 
Published: May 2021

PurposeThis paper aims to investigate the nexus between banks’ foreign assets and sovereign default risk in a panel of 15 developed economies. The empirical evidence suggests that banks’ foreign exposure is an important determinant of sovereign default probability.Design/methodology/approachUsing data from the consolidated banking statistics (total foreign claims on ultimate risk basis) by the Bank of International Settlements, the author constructs a measure of bank international exposure to peer countries. This measure is then used as the target variable in a panel regression for sovereign credit default swaps. The model includes 15 European and non-European developed economies. Identification is discussed extensively in the paper.FindingsQuantitatively, a 1% increase in banks’ cross-border claims increases sovereign default risk by about 0.19%. The relationship is weaker when banks are more capitalised. On the other hand, governments are more vulnerable to credit risk spillovers from banks’ international portfolios when having higher debt to GDP ratios.Originality/valueTo the best of the author’s knowledge, this is the first paper that attempts explicitly to establish an empirical connection between banks’ international assets and sovereign default risk. To the author’s opinion, this paper represents a contribution to our understanding of how sovereign credit risk spills over across countries. It also extends significantly the existing literature on the determinants of sovereign risk (that primarily focused on fundamentals, market characteristics – such as liquidity – and global factors). This paper ultimately sheds some new light on the role of intermediaries in the international transmission of credit risk, also adding to today’s discussion about the linkages between banks and sovereigns.
Factors affecting the selection of effective cost control techniques in the UK construction industry
Authors: Oyegoke Adekunle Sabitu , Powell Robert , Ajayi Saheed , Godawatte Godawatte Arachchige Gimhan Rathnagee , Akenroye Temidayo 
Published: May 2021

Purpose
This paper aims to identify and analyse the factors affecting the selection of effective cost control techniques in the UK construction industry and assess their importance. The study examines these key areas; the factors that have significant impacts on cost overruns, the most effective cost control techniques and the factors for selecting cost control techniques for a project.

Design/methodology/approach
The study relies on a mixed-method research approach; a qualitative exploration of the most effective cost control techniques and the factors affecting the selection of cost control techniques, followed by a questionnaire survey and follow-up interviews. Relative importance index (RII) is used for ranking the factors.

Findings
The budgeting technique is ranked first with-0.821RII, followed by cost forecasting-0.800RII and cashflow monitoring-00.733RII, as the most effective cost control techniques. On factors that influenced the choice of the techniques used, cost information/cost-related factors are ranked first with-0.611RII, followed by the size of the company-0.509RII and the effectiveness of the technique-0.572RII.

Research limitations/implications
Although the scope of the study was limited to the UK construction industry, the results could be interpreted for critical learning in other developed/developing countries.

Originality/value
Identifying and ranking the factors affecting the selection of effective cost control techniques in the UK construction industry has been the focal point of this study. The study also proposes a simple but effective model which can be used for critical learning on mitigating cost overruns and the effective use of cost control techniques in the construction industry.
Brand familiarity vs profit-sharing rate: which has a stronger impact on Muslim customers’ intention to invest in an Islamic bank?
Authors: Hati Sri Rahayu Hijrah , Putri Niken Iwani Surya , Daryanti Sri , Wibowo Sigit Sulistiyo , Safira Anya , Setyowardhani Hapsari 
Published: May 2021

Purpose
The purpose of this study is to examine the impact of brand familiarity and profit-sharing rate on Muslim customers’ brand trust, perceived financial risk, perceived value and intention to invest in an Islamic bank.

Design/methodology/approach
A between-subjects experimental design was applied in the study. Six experiments involving two brand familiarity levels and three profit-sharing rates were conducted using a total of 217 samples. Randomization was applied in the study, which generated unequal sample sizes for each group of experiments.

Findings
The findings of this experimental study demonstrated that Muslim customers’ familiarity with the bank’s brand has a significant impact on their brand trust and intention to invest in an Islamic bank. The study also found that the profit-sharing rate has a significant impact on the perceived value both with and without interaction with brand familiarity.

Research limitations/implications
The current study applies an independent measured design or a between-subjects experimental design, that resulted in unequal sample sizes. In addition, the study also does not control for the types of bank accounts owned by respondents. The design may invite the presence of confounding variables that exist due to individual differences and environmental variables.

Practical implications
The results show that Islamic bank managers should care about the brand familiarity issue, which strongly influences customers’ brand trust and customer intention to invest in an Islamic bank. In addition, Islamic bank managers should pay attention to the profit-sharing rate given to customers, as it interacts with brand familiarity in influencing customers’ perceived value.

Originality/value
This study examined the impact of brand familiarity and profit-sharing rate on Muslim consumers’ brand trust, perceived risk, perceived value and intention to save in an Islamic bank. The paper provides a shred of empirical evidence to the theoretical relationship between the subjective and objective cues that influence the formation of customers’ trust, perceived financial risk, perceived value and intention in the Islamic bank context.
Measures to combat money laundering and terrorist financing in Palestine
Authors: Firas Murrar 
Published: May 2021

Purpose
The purpose of this paper is to analyse the evolution of anti-money laundering/combating the financing of terrorism (AML/CFT) procedures in Palestine since 2004 in accordance with the standards issued by the Financial Action Task Force (FATF).

Design/methodology/approach
This study is qualitative in nature and involves studying the most important improvements made by Palestinian authorities in the state’s legislative and institutional frameworks to enhance the AML/CFT regime.

Findings
Palestine has established the necessary legal basis to combat money laundering and terrorist financing crimes. At the institutional level, the Financial Follow-up Unit was granted all the required powers of Financial Intelligence Units. The National Committee for Combating Money Laundering and Financing Terrorism has also played a vital role in issuing policies and plans to respond to the outcomes of the National Risk Assessment process. In contrast, a number of challenges still exist mainly with respect to the political factors and their expected consequences on the process of preparing for and conducting the mutual evaluation process for Palestine.

Originality/value
This study focusses on the AML/CFT efforts in Palestine owing to the nature and specificity of the Palestinian situation, as Palestine’s AML/CFT procedures have not been subject to any previous mutual evaluation process by the MENAFATF. Such efforts have rarely addressed the Palestinian case, making this study important to researchers and those interested in this field.
Nigeria’s upstream petroleum industry anti-corruption legal framework: the necessity for overhauling and enrichment
Authors: Olujobi Olusola Joshua 
Published: May 2021

Purpose
This study aims to investigate why anti-corruption statutes are not efficient in Nigeria’s upstream petroleum industry.

Design/methodology/approach
This study is a doctrinal legal research that embraces a point-by-point comparative methodology with a library research technique.

Findings
This study reveals that corruption strives on feeble implementation of anti-corruption legal regime and the absence of political will in offering efficient regulatory intervention. Finally, this study finds that anti-corruption organisations in Nigeria are not efficient due to non-existence of the Federal Government’s political will to fight corruption, insufficient funds and absence of stringent implementation of the anti-corruption legal regime in the country.

Research limitations/implications
Investigations reveal during this study that Nigerian National Petroleum Corporation (NNPC) operations are characterised with poor record-keeping, lack of accountability as well as secrecy in the award of oil contracts, oil licence, leases and other financial transactions due to non-disclosure or confidentiality clauses contained in most of these contracts. Also, an arbitration proceeding limit access to their records and some of these agreements under contentions. This has also limited the success of this research work and generalising its findings.

Practical implications
This study recommends, among other reforms, soft law technique and stringent execution of anti-corruption statutes. This study also recommends increment in financial appropriation to Nigeria’s anti-corruption institutions, taking into consideration the finding that a meagre budget is a drawback.

Social implications
This study reveals that corruption strives on feeble implementation of anti-corruption legal regime and the absence of political will in offering efficient regulatory intervention. Corruption flourishes due to poor enforcement of anti-corruption laws and the absence of political will in offering efficient regulatory intervention by the government.

Originality/value
The study advocates the need for enhancement of anti-corruption agencies' budgets taking into consideration the finding that meagres budgets are challenge of the agencies.