bluuebunny/crossref_metadata_2025_split huggingface dataset:
Modelling the imposed magnetospheres of Mars-like exoplanets: star–planet interactions and atmospheric losses
Authors: Basak Arnab , Nandy Dibyendu 
Published: January 2021

ABSTRACT
Based on 3D compressible magnetohydrodynamic simulations, we explore the interactions between the magnetized wind from a solar-like star and a Mars-like planet – with a gravitionally stratified atmosphere – that is either non-magnetized or hosts a weak intrinsic dipolar field. The primary mechanism for the induction of a magnetosphere around a non-magnetized conducting planet is the pile-up of stellar magnetic fields in the day-side region. The magnetopause stand-off distance decreases as the strength of the planetary dipole field is lowered and saturates to a minimum value for the case of a planet with no magnetic field. Global features such as bow shock, magnetosheath, magnetotail, and strong current sheets are observed in the imposed magnetosphere. We explore variations in atmospheric mass loss rates for different stellar wind strengths to understand the impact of stellar magnetic activity and plasma winds – and their evolution – on (exo)planetary habitability. In order to simulate a case analogous to the present-day Mars, a planet without atmosphere is considered. Our simulations are found to be in good agreement with observational data from Mars Global Surveyor and Mars Atmosphere and Volatile EvolutioN missions and is expected to complement observations from the Emirates (Hope) Mars Mission, China's Tianwen-1 and NASA's Mars 2020 Perseverance mission.
Kozai–Lidov oscillations triggered by a tilt instability of detached circumplanetary discs
Authors: Martin Rebecca G , Zhu Zhaohuan , Armitage Philip J , Yang Chao-Chin , Baehr Hans 
Published: January 2021

ABSTRACT
Circumplanetary discs can be linearly unstable to the growth of disc tilt in the tidal potential of the star–planet system. We use 3D hydrodynamical simulations to characterize the disc conditions needed for instability, together with its long-term evolution. Tilt growth occurs for disc aspect ratios, evaluated near the disc outer edge, of H/r ≳ 0.05, with a weak dependence on viscosity in the wave-like regime of warp propagation. Lower mass giant planets are more likely to have circumplanetary discs that satisfy the conditions for instability. We show that the tilt instability can excite the inclination to above the threshold where the circumplanetary disc becomes unstable to Kozai–Lidov (KL) oscillations. Dissipation in the KL unstable regime caps further tilt growth, but the disc experiences large oscillations in both inclination and eccentricity. Planetary accretion occurs in episodic accretion events. We discuss implications of the joint tilt–KL instability for the detectability of circumplanetary discs, for the obliquity evolution of forming giant planets, and for the formation of satellite systems.
Structural basis of diversity and homodimerization specificity of zinc-finger-associated domains in Drosophila
Authors: Bonchuk Artem , Boyko Konstantin , Fedotova Anna , Nikolaeva Alena , Lushchekina Sofya , Khrustaleva Anastasia , Popov Vladimir , Georgiev Pavel 
Published: January 2021

AbstractIn arthropods, zinc finger-associated domains (ZADs) are found at the N-termini of many DNA-binding proteins with tandem arrays of Cys2-His2 zinc fingers (ZAD-C2H2 proteins). ZAD-C2H2 proteins undergo fast evolutionary lineage-specific expansion and functional diversification. Here, we show that all ZADs from Drosophila melanogaster form homodimers, but only certain ZADs with high homology can also heterodimerize. CG2712, for example, is unable to heterodimerize with its paralog, the previously characterized insulator protein Zw5, with which it shares 46% homology. We obtained a crystal structure of CG2712 protein's ZAD domain that, in spite of a low sequence homology, has similar spatial organization with the only known ZAD structure (from Grauzone protein). Steric clashes prevented the formation of heterodimers between Grauzone and CG2712 ZADs. Using detailed structural analysis, site-directed mutagenesis, and molecular dynamics simulations, we demonstrated that rapid evolutionary acquisition of interaction specificity was mediated by the more energy-favorable formation of homodimers in comparison to heterodimers, and that this specificity was achieved by multiple amino acid substitutions resulting in the formation or breaking of stabilizing interactions. We speculate that specific homodimerization of ZAD-C2H2 proteins is important for their architectural role in genome organization.
Bacteria have numerous distinctive groups of phage–plasmids with conserved phage and variable plasmid gene repertoires
Authors: Pfeifer Eugen , Moura de Sousa Jorge A , Touchon Marie , Rocha Eduardo P C 
Published: January 2021

Abstract
Plasmids and temperate phages are key contributors to bacterial evolution. They are usually regarded as very distinct. However, some elements, termed phage–plasmids, are known to be both plasmids and phages, e.g. P1, N15 or SSU5. The number, distribution, relatedness and characteristics of these phage–plasmids are poorly known. Here, we screened for these elements among ca. 2500 phages and 12000 plasmids and identified 780 phage–plasmids across very diverse bacterial phyla. We grouped 92% of them by similarity of gene repertoires to eight defined groups and 18 other broader communities of elements. The existence of these large groups suggests that phage–plasmids are ancient. Their gene repertoires are large, the average element is larger than an average phage or plasmid, and they include slightly more homologs to phages than to plasmids. We analyzed the pangenomes and the genetic organization of each group of phage–plasmids and found the key phage genes to be conserved and co-localized within distinct groups, whereas genes with homologs in plasmids are much more variable and include most accessory genes. Phage–plasmids are a sizeable fraction of the sequenced plasmids (∼7%) and phages (∼5%), and could have key roles in bridging the genetic divide between phages and other mobile genetic elements.
Experts’ Perceptions of and Suggestions for Cigar Warning Label Messages and Pictorials
Authors: Wackowski Olivia A , Jeong Michelle , Schroth Kevin R J , Rashid Mariam , Delnevo Cristine D 
Published: January 2021

Abstract

Introduction
Warning labels can be effective tools to inform the public about tobacco risks. However, tobacco warning research has been largely cigarette-centric. This formative study explores potential directions for improving the current warnings for cigars.

Aims and Methods
Between June and August 2019, we conducted interviews with 10 experts about the current cigar warning statements and requirements, the inclusion of pictorials in cigar warnings, and legal issues. Additionally, experts viewed and discussed concept images to pair with existing warning statements, including realistic photographic images and symbolic images (eg, icons, caution symbols).

Results
Experts agreed that cigar warnings should be strengthened (eg, by increasing their size and use of pictorials) to better leverage their potential impact and maintain warning parity with cigarettes. However, perceived challenges exist given the wide variety of cigar products, product terminology, and use patterns. Experts agreed that photographic pictorials of health effects are likely to be more salient, informative, and effective for behavior change than symbolic ones, but may be vulnerable to legal challenges. Symbolic images used in warnings may obtain attention in a less “controversial” way, but may be considered less serious and “factually accurate,” increasing legal risks. Experts indicate that cigar educational campaigns can complement warnings and address additional themes, especially aimed at youth, including exposure to chemicals and flavorings and misperceptions that cigars are “natural.”

Conclusions
Additional consumer research is needed to examine the potential public health impact of improving cigar warnings and to support regulatory efforts.

Implications
This study provides feedback from tobacco control experts about the importance of cigar warning labels and directions for future research and improvements. Experts support increasing the size of cigar warnings and adopting pictorials to improve cigar warning impact and maintain warning parity with cigarettes. Experts agreed that photographic pictorials of health effects are likely to be more salient and effective for behavior change than symbolic ones. Given legal challenges, experts discussed potentially prioritizing warning improvements for those cigars used most frequently as cigarette alternatives and by youth. Overall, more cigar warning research is needed to support regulatory efforts.
Winter zooplankton dynamics in the English Channel and southern North Sea: trends and drivers from 1991 to 2013
Authors: Dudeck Tim , Rohlf Norbert , Möllmann Christian , Hufnagl Marc 
Published: January 2021

Abstract
Winter has long been regarded as a period of minor importance in marine zooplankton ecology with static, low concentrations and growth rates of organisms. Yet, there is growing evidence that winter conditions influence spring bloom strength. With rising water temperatures, growing importance of fish larvae survival during winter and the lack of data to parameterize ecosystem models for this period, research focus shifted on winter zooplankton ecology. To enable looking into past changes in winter zooplankton ecology, we established a new winter zooplankton time series based on samples collected in the eastern English Channel and southern North Sea by the International Herring Larvae Survey since 1988. Four areas of the study region were identified containing different congregations of zooplankton. Overall, zooplankton size decreased while total zooplankton abundance increased, reaching its maximum in 2011. Zooplankton abundance dynamics were mainly related to temperature, chlorophyll a concentration and North Atlantic Oscillation index. Depth and Atlantic water inflow strongly influenced zooplankton size. Increased chlorophyll a concentration and high abundances of small copepods indicated bottom-up controlled secondary production since 2010 and a possible winter bloom in 2011. Based on the analysed parameters, no relation between herring larvae abundance and zooplankton or environmental drivers was determined.
Insights into the mechanisms of transport and regulation of the arabidopsis high-affinity K+ transporter HAK51
Authors: Ródenas Reyes , Ragel Paula , Nieves-Cordones Manuel , Martínez-Martínez Almudena , Amo Jesús , Lara Alberto , Martínez Vicente , Quintero Francisco J , Pardo Jose M , Rubio Francisco 
Published: January 2021

Abstract
The high-affinity K+ transporter HAK5 from Arabidopsis (Arabidopsis thaliana) is essential for K+ acquisition and plant growth at low micromolar K+ concentrations. Despite its functional relevance in plant nutrition, information about functional domains of HAK5 is scarce. Its activity is enhanced by phosphorylation via the AtCIPK23/AtCBL1-9 complex. Based on the recently published three-dimensionalstructure of the bacterial ortholog KimA from Bacillus subtilis, we have modeled AtHAK5 and, by a mutational approach, identified residues G67, Y70, G71, D72, D201, and E312 as essential for transporter function. According to the structural model, residues D72, D201, and E312 may bind K+, whereas residues G67, Y70, and G71 may shape the selective filter for K+, which resembles that of K+shaker-like channels. In addition, we show that phosphorylation of residue S35 by AtCIPK23 is required for reaching maximal transport activity. Serial deletions of the AtHAK5 C-terminus disclosed the presence of an autoinhibitory domain located between residues 571 and 633 together with an AtCIPK23-dependent activation domain downstream of position 633. Presumably, autoinhibition of AtHAK5 is counteracted by phosphorylation of S35 by AtCIPK23. Our results provide a molecular model for K+ transport and describe CIPK-CBL-mediated regulation of plant HAK transporters.
Plasma membrane phylloquinone biosynthesis in nonphotosynthetic parasitic plants
Authors: Gu Xi , Chen Ing-Gin , Harding Scott A , Nyamdari Batbayar , Ortega Maria A , Clermont Kristen , Westwood James H , Tsai Chung-Jui 
Published: January 2021

Abstract
Nonphotosynthetic holoparasites exploit flexible targeting of phylloquinone biosynthesis to facilitate plasma membrane redox signaling. Phylloquinone is a lipophilic naphthoquinone found predominantly in chloroplasts and best known for its function in photosystem I electron transport and disulfide bridge formation of photosystem II subunits. Phylloquinone has also been detected in plasma membrane (PM) preparations of heterotrophic tissues with potential transmembrane redox function, but the molecular basis for this noncanonical pathway is unknown. Here, we provide evidence of PM phylloquinone biosynthesis in a nonphotosynthetic holoparasite Phelipanche aegyptiaca. A nonphotosynthetic and nonplastidial role for phylloquinone is supported by transcription of phylloquinone biosynthetic genes during seed germination and haustorium development, by PM-localization of alternative terminal enzymes, and by detection of phylloquinone in germinated seeds. Comparative gene network analysis with photosynthetically competent parasites revealed a bias of P. aegyptiaca phylloquinone genes toward coexpression with oxidoreductases involved in PM electron transport. Genes encoding the PM phylloquinone pathway are also present in several photoautotrophic taxa of Asterids, suggesting an ancient origin of multifunctionality. Our findings suggest that nonphotosynthetic holoparasites exploit alternative targeting of phylloquinone for transmembrane redox signaling associated with parasitism.
Consumer acceptance and willingness to pay for cow housing systems in eight European countries
Authors: Waldrop Megan E , Roosen Jutta 
Published: January 2021

AbstractFree walk housing systems aim to address major animal health issues for dairy cows and support higher animal welfare. The purpose of this study is to estimate consumer acceptance of different housing systems and willingness to pay (WTP) for milk from tie-stall, cubicle, compost-bedded, and artificial floor housing systems in addition to attitudes toward animal welfare. Focus groups were held to identify the pertinent concepts for an online quantitative survey conducted in eight European countries. A discrete choice experiment was included for estimating WTP. Consumers are found to care about animal welfare and grazing. Results indicate consumers like the compost-bedded system the best followed by the artificial floor system; however, no positive WTP for these systems was found. Consumers seem to find the current labeling rules regarding organic production and grazing sufficiently informative. The results can help producers make more informed investment and marketing decisions regarding cow husbandry.
Defining the optimal disease duration of early diffuse systemic sclerosis for clinical trial design
Authors: Domsic Robyn T , Gao Shiyao , Laffoon Maureen , Wisniewski Steven , Zhang Yuqing , Steen Virginia , Lafyatis Robert , Medsger Thomas A 
Published: January 2021

Abstract

Objectives
Clinical trials in early diffuse cutaneous systemic sclerosis (SSc) using the modified Rodnan skin score (mRSS) as the primary outcome measure have most often been negative. We wanted to assess how the definition of disease onset (first SSc manifestation vs first non-Raynaud manifestation) and varying lengths of disease duration at trial entry as an inclusion criteria functioned. Our objective was to optimize trial inclusion criteria

Methods
We used the prospective, observational University of Pittsburgh Scleroderma Cohort to identify early diffuse SSc patients first evaluated between 1980 and 2015. All had &lt;3 years from first SSc (n = 481) or first non-Raynaud manifestation (n = 514) and three or more mRSS scores. We used descriptive, survival and group-based trajectory analyses to compare the different definitions of disease onset and disease duration as inclusion criteria for clinical trials.

Results
There was no appreciable difference between using first SSc manifestation compared with first non-Raynaud manifestation as the definition of disease onset. Compared with other disease durations, &lt;18 months of disease had &gt;70% of patients fitting into trajectories with worsening cutaneous disease over 6 months of follow-up. Longer disease durations demonstrated the majority of patients with trajectories showing an improvement in mRSS (regression to the mean) over 6 months.

Conclusions
Regardless of whether the first SSc or first non-Raynaud manifestation is used to define disease onset, duration of &lt;18 months at enrolment is preferable. A longer disease duration criterion more frequently results in regression to the mean of the mRSS score, and likely contributes to negative trial outcomes.
Post-translational modifications in T cells in systemic erythematosus lupus
Authors: Yang Fan , Lin Jin , Chen Weiqian 
Published: January 2021

AbstractSystemic erythematosus lupus (SLE) is a classic autoimmune disease characterized by multiple autoantibodies and immune-mediated tissue damage. The aetiology of this disease is still unclear. A new drug, belimumab, which acts against the B-lymphocyte stimulator (BLyS), can effectively improve the condition of SLE patients, but it cannot resolve all SLE symptoms. The discovery of novel, precise therapeutic targets is urgently needed. It is well known that abnormal T-cell function is one of the most crucial factors contributing to the pathogenesis of SLE. Protein post-translational modifications (PTMs), including phosphorylation, glycosylation, acetylation, methylation, ubiquitination and SUMOylation have been emphasized for their roles in activating protein activity, maintaining structural stability, regulating protein–protein interactions and mediating signalling pathways, in addition to other biological functions. Summarizing the latest data in this area, this review focuses on the potential roles of diverse PTMs in regulating T-cell function and signalling pathways in SLE pathogenesis, with the goal of identifying new targets for SLE therapy.
Is Cohort Representativeness Passé? Poststratified Associations of Lifestyle Risk Factors with Mortality in the UK Biobank
Authors: Stamatakis Emmanuel , Owen Katherine B. , Shepherd Leah , Drayton Bradley , Hamer Mark , Bauman Adrian E. 
Published: January 2021

Background:
The UK Biobank (UKB) has been used widely to examine associations between lifestyle risk factors and mortality outcomes. It is unknown whether the extremely low UKB response rate (5.5%) and lack of representativeness materially affects the magnitude and direction of effect estimates.

Methods:
We used poststratification to match the UKB sample to the target population in terms of sociodemographic characteristics and prevalence of lifestyle risk factors (physical inactivity, alcohol intake, smoking, and poor diet). We compared unweighted and poststratified associations between each lifestyle risk factor and a lifestyle index score with all-cause, cardiovascular disease (CVD), and cancer mortality. We also calculated the unweighted to poststratified ratio of HR (RHR) and 95% confidence interval as a marker of effect-size difference.

Results:
Of 371,974 UKB participants with no missing data, 302,009 had no history of CVD or cancer, corresponding to 3,298,958 person years of follow-up. Protective associations between alcohol use and CVD mortality observed in the unweighted UKB were substantially altered after poststratification, for example, from a hazard ratio (HR) of 0.63 (0.45–0.87) unweighted to 0.99 (0.65–1.50) poststratified for drinking ≥5 times/week versus never drinking. The magnitude of the poststratified all-cause mortality hazard ratio comparing least healthy with healthiest tertile of lifestyle risk factor index was 9% higher (95% confidence interval: 4%, 14%) than the unweighted estimates.

Conclusions:
Lack of representativeness may distort the associations of alcohol with CVD mortality, and may underestimate health hazards among those with cumulatively the least healthy lifestyles.
Health Care Satisfaction in Relation to Gender Identity
Authors: Ferrucci Katarina A. , Walubita Tubanji , Beccia Ariel L. , Ding Eric Y. , Jesdale Bill M. , Lapane Kate L. , Streed Carl G. 
Published: January 2021

Background:
Health care satisfaction is a key component of patient-centered care. Prior research on transgender populations has been based on convenience samples, and/or grouped all gender minorities into a single category.

Objective:
The objective of this study was to quantify differences in health care satisfaction among transgender men, transgender women, gender nonconforming, and cisgender adults in a diverse multistate sample.

Research Design:
Cross-sectional analysis of 2014–2018 Behavioral Risk Factor Surveillance System data from 20 states, using multivariable logistic models.

Subjects:
We identified 167,468 transgender men, transgender women, gender-nonconforming people, cisgender women, and cisgender men and compared past year health care satisfaction across these groups.

Results:
Transgender men and women had the highest prevalence of being “not at all satisfied” with the health care they received (14.6% and 8.6%, respectively), and gender-nonconforming people had the lowest prevalence of being “very satisfied” with their health care (55.7%). After adjustment for sociodemographic characteristics, transgender men were more likely to report being “not at all satisfied” with health care than cisgender men (odds ratio: 4.45, 95% confidence interval: 1.72–11.5) and cisgender women (odds ratio: 3.40, 95% confidence interval: 1.31–8.80).

Conclusions:
Findings indicate that transgender and gender-nonconforming adults report considerably less health care satisfaction relative to their cisgender peers. Interventions to address factors driving these differences are needed.
Estimated Lifetime HIV–Related Medical Costs in the United States
Authors: Bingham Adrienna , Shrestha Ram K. , Khurana Nidhi , Jacobson Evin U. , Farnham Paul G. 
Published: January 2021

Background
Lifetime cost estimates are a useful tool in measuring the economic burden of HIV in the United States. Previous estimation methods need to be updated, given improving antiretroviral therapy regimens and updated costs.

Methods
We used an updated version of the agent-based model progression and transmission of HIV (PATH) 3.0 to reflect current regimens and costs. We simulated a cohort of those infected in 2015 until the last person had died to track the lifetime costs for treatment of HIV, including HIV health care utilization costs (inpatient, outpatient, opportunistic infection prophylaxis, non-HIV medication, and emergency department), opportunistic infection treatment costs, and testing costs. We assumed a median per-person diagnosis delay of 3 years and a 3% base monthly probability of dropout from care for a base-case scenario. Additionally, we modeled a most favorable scenario (median diagnosis delay of 1 year and 1% base dropout rate) and a least favorable scenario (median diagnosis delay of 5 years and 5% base dropout rate).

Results
We estimated an average lifetime HIV-related medical cost for a person with HIV of $420,285 (2019 US$) discounted (3%) and $1,079,999 undiscounted for a median 3-year diagnosis delay and 3% base dropout rate. Our discounted cost estimate was $490,045 in our most favorable scenario and $326,411 in our least favorable scenario.

Conclusions
Lifetime per-person HIV-related medical costs depend on the time from infection to diagnosis and the likelihood of dropping out of care. Our results, which are similar to previous studies, reflect updated antiretroviral therapy regimens and costs for HIV treatment.