bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cis- and trans-acting Influences on Telomeric Position Effect in Drosophila melanogaster Detected With a Subterminal Transgene
Authors: Mason James M , Konev Alexander Y , Golubovsky Mikhail D , Biessmann Harald 
Published: April 2021

Abstract
One model of telomeric position effect (TPE) in Drosophila melanogaster proposes that reporter genes in the vicinity of telomeres are repressed by subterminal telomere-associated sequences (TAS) and that variegation of these genes is the result of competition between the repressive effects of TAS and the stimulating effects of promoters in the terminal HeT-A transposon array. The data presented here support this model, but also suggest that TPE is more complex. Activity of a telomeric white reporter gene increases in response to deletion of some or all of the TAS on the homolog. Only transgenes next to fairly long HeT-A arrays respond to this trans-interaction. HeT-A arrays of 6-18 kb respond by increasing the number of dark spots on the eye, while longer arrays increase the background eye color or increase the number of spots sufficiently to cause them to merge. Thus, expression of a subtelomeric reporter gene is influenced by the telomere structure in cis and trans. We propose that the forces involved in telomere length regulation in Drosophila are the underlying forces that manifest themselves as TPE. In the wild-type telomere TAS may play an important role in controlling telomere elongation by repressing HeT-A promoter activity. Modulation of this repression by the homolog may thus regulate telomere elongation.
The Drosophila melanogaster sir2+ Gene Is Nonessential and Has Only Minor Effects on Position-Effect Variegation
Authors: Åström Stefan U , Cline Thomas W , Rine Jasper 
Published: April 2021

Abstract
Five Drosophila melanogaster genes belong to the highly conserved sir2 family, which encodes NAD+-dependent protein deacetylases. Of these five, dsir2+ (CG5216) is most similar to the Saccharomyces cerevisiae SIR2 gene, which has profound effects on chromatin structure and life span. Four independent Drosophila strains were found with P-element insertions near the dsir2 transcriptional start site as well as extraneous linked recessive lethal mutations. Imprecise excision of one of these P elements (PlacW 07223) from a chromosome freed of extraneous lethal mutations produced dsir217, a null intragenic deletion allele that generates no DSIR2 protein. Contrary to expectations from the report by Rosenberg and Parkhurst on their P-mobilization allele dSir2ex10, homozygosity for dsir217 had no apparent deleterious effects on viability, developmental rate, or sex ratio, and it fully complemented sir2ex10. Moreover, through a genetic test, we ruled out the reported effect of dSir2ex10 on Sex-lethal expression. We did observe a modest, strictly recessive suppression of whitem4 position-effect variegation and a shortening of life span in dsir2 homozygous mutants, suggesting that dsir2 has some functions in common with yeast SIR2.
The Drosophila chk2 Gene loki Is Essential for Embryonic DNA Double-Strand-Break Checkpoints Induced in S Phase or G2
Authors: Masrouha Nisrine , Yang Long , Hijal Sirine , Larochelle Stéphane , Suter Beat 
Published: April 2021

Abstract
Cell cycle checkpoints are signal transduction pathways that control the order and timing of cell cycle transitions, ensuring that critical events are completed before the occurrence of the next cell cycle transition. The Chk2 family of kinases is known to play a central role in mediating the cellular responses to DNA damage or DNA replication blocks in various organisms. Here we show through a phylogenetic study that the Drosophila melanogaster serine/threonine kinase Loki is the homolog of the yeast Mek1p, Rad53p, Dun1p, and Cds1 proteins as well as the human Chk2. Functional analyses allowed us to conclude that, in flies, chk2 is involved in monitoring double-strand breaks (DSBs) caused by irradiation during S and G2 phases. In this process it plays an essential role in inducing a cell cycle arrest in embryonic cells. Our results also show that, in contrast to C. elegans chk2, Drosophila chk2 is not essential for normal meiosis and recombination, and it also appears to be dispensable for the MMS-induced DNA damage checkpoint and the HU-induced DNA replication checkpoint during larval development. In addition, Drosophila chk2 does not act at the same cell cycle phases as its yeast homologs, but seems rather to be involved in a pathway similar to the mammalian one, which involves signaling through the ATM/Chk2 pathway in response to genotoxic insults. As mutations in human chk2 were linked to several cancers, these similarities point to the usefulness of the Drosophila model system.
Nuclear Gene Genealogies Reveal Historical, Demographic and Selective Factors Associated With Speciation in Field Crickets
Authors: Broughton Richard E , Harrison Richard G 
Published: April 2021

Abstract
Population genetics theory predicts that genetic drift should eliminate shared polymorphism, leading to monophyly or exclusivity of populations, when the elapsed time between lineage-splitting events is large relative to effective population size. We examined patterns of nucleotide variation in introns at four nuclear loci to relate processes affecting the history of genes to patterns of divergence among natural populations and species. Ancestral polymorphisms were shared among three recognized species, Gryllus firmus, G. pennsylvanicus, and G. ovisopis, and genealogical patterns suggest that successive speciation events occurred recently and rapidly relative to effective population size. High levels of shared polymorphism among these morphologically, behaviorally, and ecologically distinct species indicate that only a small fraction of the genome needs to become differentiated for speciation to occur. Among the four nuclear gene loci there was a 10-fold range in nucleotide diversity, and patterns of polymorphism and divergence suggest that natural selection has acted to maintain or eliminate variation at some loci. While nuclear gene genealogies may have limited applications in phylogeography or other approaches dependent on population monophyly, they provide important insights into the historical, demographic, and selective forces that shape speciation.
Fine Mapping of Complex Trait Genes Combining Pedigree and Linkage Disequilibrium Information: A Bayesian Unified Framework
Authors: Pérez-Enciso Miguel 
Published: April 2021

Abstract
We present a Bayesian method that combines linkage and linkage disequilibrium (LDL) information for quantitative trait locus (QTL) mapping. This method uses jointly all marker information (haplotypes) and all available pedigree information; i.e., it is not restricted to any specific experimental design and it is not required that phases are known. Infinitesimal genetic effects or environmental noise (“fixed”) effects can equally be fitted. A diallelic QTL is assumed and both additive and dominant effects can be estimated. We have implemented a combined Gibbs/Metropolis-Hastings sampling to obtain the marginal posterior distributions of the parameters of interest. We have also implemented a Bayesian variant of usual disequilibrium measures like D′ and r2 between QTL and markers. We illustrate the method with simulated data in “simple” (two-generation full-sib families) and “complex” (four-generation) pedigrees. We compared the estimates with and without using linkage disequilibrium information. In general, using LDL resulted in estimates of QTL position that were much better than linkage-only estimates when there was complete disequilibrium between the mutant QTL allele and the marker. This advantage, however, decreased when the association was only partial. In all cases, additive and dominant effects were estimated accurately either with or without disequilibrium information.
A Tail’s Tale: Biomechanical Roles of Dorsal Thoracic Spine of Barnacle Nauplii
Authors: Branam Emily N , Wong Jin Yung , Chan Benny K K , Chan Kit Yu Karen 
Published: April 2021

AbstractMany marine invertebrates have complex life histories that begin with a planktonic larval stage. Similar to other plankton, these larval invertebrates often possess protruding body extensions, but their function beyond predator deterrence is not well-documented. For example, the planktonic nauplii of crustaceans have spines. Using the epibiotic pedunculate barnacle Octolasmis spp., we investigated how the dorsal thoracic spine affects swimming and fluid disturbance by comparing nauplii with their spines partially removed against those with intact spines. Our motion analysis showed that amputated Octolasmis spp. swam slower, in jerkier trajectories, and were less efficient per stroke cycle than those with intact spines. Amputees showed alterations in limb beat pattern: larger beat amplitude, increased phase lag, and reduced contralateral symmetry. These changes might partially help increase propulsive force generation and streamline the flow, but were insufficient to restore full function. Particle image velocimetry further showed that amputees had a larger relative area of influence, implying elevated risk by rheotactic predator. Body extensions and their interactions with limb motion play important biomechanical roles in shaping larval performance, which likely influences the evolution of form.
The role of taxonomic expertise in interpretation of metabarcoding studies
Authors: Pappalardo Paula , Collins Allen G , Pagenkopp Lohan Katrina M , Hanson Kate M , Truskey Sarit B , Jaeckle William , Ames Cheryl Lewis , Goodheart Jessica A , Bush Stephanie L , Biancani Leann M , Strong Ellen E , Vecchione Michael , Harasewych M G , Reed Karen , Lin Chan , Hartil Elise C , Whelpley Jessica , Blumberg Jamie , Matterson Kenan , Redmond Niamh E , Becker Allison , Boyle Michael J , Osborn Karen J 
Published: April 2021

Abstract
The performance of DNA metabarcoding approaches for characterizing biodiversity can be influenced by multiple factors. Here, we used morphological assessment of taxa in zooplankton samples to develop a large barcode database and to assess the congruence of taxonomic identification with metabarcoding under different conditions. We analysed taxonomic assignment of metabarcoded samples using two genetic markers (COI, 18S V1–2), two types of clustering into molecular operational taxonomic units (OTUs, ZOTUs), and three methods for taxonomic assignment (RDP Classifier, BLASTn to GenBank, BLASTn to a local barcode database). The local database includes 1042 COI and 1108 18S (SSU) barcode sequences, and we added new high-quality sequences to GenBank for both markers, including 109 contributions at the species level. The number of phyla detected and the number of taxa identified to phylum varied between a genetic marker and among the three methods used for taxonomic assignments. Blasting the metabarcodes to the local database generated multiple unique contributions to identify OTUs and ZOTUs. We argue that a multi-marker approach combined with taxonomic expertise to develop a curated, vouchered, local barcode database increases taxon detection with metabarcoding, and its potential as a tool for zooplankton biodiversity surveys.
Durable mechanical circulatory support in patients with heart failure with preserved ejection fraction
Authors: Lanmueller Pia , Eulert-Grehn Jaime-Juergen , Schoenrath Felix , Pieske Burkert , Mulzer Johanna , Mueller Marcus , Falk Volkmar , Potapov Evgenij 
Published: April 2021

Abstract
Only few patients with heart failure with preserved ejection fraction undergo durable mechanical circulatory support implantation. We identified three patients with heart failure with preserved ejection fraction who needed durable mechanical circulatory support as bridge-to-transplant therapy. In two patients with hypertrophic cardiomyopathy, the hypertrophic papillary muscles and myocardium were resected to allow for subsequent left ventricular assist device implantation. In one patient, all visible parts of the mitral valve were additionally resected. The third patient with restrictive cardiomyopathy underwent Berlin Heart Excor BVAD implantation with left atrial cannulation.
The Meaning of Participation in Transitional Justice: A Conceptual Proposal for Empirical Analysis
Authors: Evrard Elke , Mejía Bonifazi Gretel , Destrooper Tine 
Published: April 2021

Abstract
Much has been written about the potential of participatory approaches to entrench and expand transitional justice processes. Yet, evidence-based research on how to understand, organize and evaluate victim participation has lagged behind. Empirical research, moreover, often lacks an explicit conceptualization of participation or adopts existing models that start from an institutional perspective and that normatively hierarchize forms and functions of participation. This article, instead, proposes an actor-oriented analytical framework that outlines participants’ trajectories throughout the transitional justice ecosystem. This framework invites a more rigorous investigation of (a) participants’ identities and interests, (b) the spaces they navigate, (c) the relation between various interests, spaces and temporalities and (d) the open-ended nature of outcomes. We apply this framework to Guatemalan indigenous women’s quest for redress for conflict-related sexual violence. The framework facilitates a different way of understanding impact, rooted in local actors’ multidirectional, context-specific and non-linear engagement with transitional justice.
Emotional reactions towards COVID-19 among persons with diabetes
Authors: Shinan-Altman Shiri , Levkovich Inbar 
Published: April 2021

Abstract
The emotional impact of the coronavirus disease 2019 (COVID-19) pandemic on populations at large is emerging in the literature. However, the emotional response of persons with diabetes to the pandemic is only now beginning to emerge. Therefore this study aimed to identify factors contributing to emotional reactions towards this pandemic among persons with diabetes. A total of 205 persons with diabetes participated in this cross-sectional online survey between 14 May and 22 June 2020. Participants completed measures of emotional reactions towards COVID-19, perceived diabetes status, perceived susceptibility, knowledge about COVID-19, sense of mastery and sociodemographic questionnaires. Statistical analyses included Pearson correlations and regression analysis. According to the results, the mean score of negative emotional reactions towards COVID-19 was 3.45 (standard deviation 1.11, range 1–5), meaning that the score was relatively high. Higher levels of negative emotional reactions towards COVID-19 were associated with higher perceived susceptibility, greater knowledge about COVID-19 and a lower sense of mastery. The study's findings emphasize the need to communicate ongoing knowledge regarding COVID-19 and diabetes as well as to provide persons with diabetes with the necessary emotional support related to coping with diabetes and COVID-19.
International, national and local trends in the spread of COVID-19: a geographic view of COVID-19 spread and the role to be played by coproduction
Authors: Schmidt Peter , Nelson Eugene C , Kearney Gregory , Kraft Sally , Oliver Brant J 
Published: April 2021

Abstract

Background
COVID-19, a respiratory disease caused by the SARS-CoV-2 virus, emerged in 2019 and led to a worldwide pandemic in 2020. The COVID-19 pandemic has been a massive natural experiment in the formation of mitigation strategies to prevent cases and to provide effective healthcare for those afflicted. Regional differences in the impact of the pandemic on morbidity and mortality have been driven by political and regional differences in the coproduction of public health and social policy. We explored the United States (US) experience of COVID-19 for trends and correlations with other nations and also at the national, regional, state and local levels.

Objective
To identify geographic and temporal trends in the spread of COVID-19 in the United States.

Methods
Population data on COVID-19 cases and mortality were acquired on a daily basis from multiple publicly available databases, including the New York Times and Johns Hopkins University. At each geographic level (national, state and county), geographic entities’ reported cases were evaluated for correlations using linear least-squares methods to identify patterns of correlation in the cases independent of scale. We evaluated for two specific characteristics: (i) the nature of the curvature of the line linking across percentile scores, ranging from concave to convex and (ii) the area under this curve, indicating how effectively a selected region (nation, state and county) is linked to its entire containing unit (world, country and state). We used this approach to identify three distinct COVID behavior phenotypes, each of which consisted of a number of states in the USA.

Results
We found that COVID activity in the USA follows a unique trend compared to other countries and that within the USA during the first year of the pandemic, three initial COVID phenotypes emerged: (i) the metropolitan outbreak (early outbreak phenotype); (ii) the regional outbreak (summer peak phenotype) and (iii) trans-regional outbreak (fall/winter peak phenotype), which, taken in sum, represent the overall USA national trend. Each phenotype has specific behavioral characteristics and is composed of a cluster of different states experiencing different conditions.

Conclusion
Our findings suggest a new opportunity for public health strategy in the pandemic, namely to apply targeted public health approaches to address the specific needs of each phenotype. In the future, we should create databases that capture key health and hardship data elements at the smallest geographic level possible and use these to track trends, predict the future and apply targeted coproduction approaches to more effectively and efficiently safeguard population health, economic vitality and social well-being.
Erylysin A inhibits cytokinesis in Escherichia coli by binding with cardiolipin
Authors: Sakihara Tomoko , Takiguchi Naoko , Uzawa Hikari , Serizawa Rika , Kobayashi Tetsuyuki 
Published: April 2021

Abstract
Cardiolipin (CL) localizes to curved membranes such as cristae in mitochondria as well as cell poles and division sites in rod-shaped bacteria. CL is believed to stabilize the membrane curvature by localizing to sites of negative curvature. However, this hypothesis has not been tested because of a lack of appropriate tools to distinguish CL inside and outside lipid bilayers. In this study, we provided the first evidence that CL localized to regions of negative curvature in Escherichia coli using the novel CL probe erylysin A-EGFP (EryA-EGFP). Staining in E.coli illustrated that CL localized to the inner leaflets at cell poles and the outer leaflets at division sites. Furthermore, we revealed that EryA-EGFP inhibited cytokinesis. We propose that cytokinesis completes after CL in the outer leaflets transfers to the inner leaflets at division sites by inspecting the mechanism of inhibition of cytokinesis. Moreover, the cytoskeletal protein RodZ was abnormally distributed when cytokinesis was inhibited by EryA-EGFP, suggesting that RodZ participates in cytokinesis. In summary, we revealed the detailed distribution of CL and proposed a new model of cytokinesis.
Burn Specific Health Scale – Brief – Brazil and International Classification of Functioning, Disability and Health in Burn Patients
Authors: Nehme Passos Maria Claudia , Gragnani Alfredo , Sarto Piccolo Mônica , Daher Ricardo Piccolo , Santana Cordeiro Eduardo , Masako Ferreira Lydia 
Published: April 2021

Abstract
With better burn trauma survival rates, quality of life and functionality have become important outcomes in the evaluation of burn patients. The objective of this study was to evaluate the quality of life of burn survivors using the Burn Specific Health Scale-Brief-Br and their function and health using the International Classification of Functioning Disability and Health (ICF) in order to assess whether there is a correlation in the results obtained between the two instruments. A cross-sectional study with 80 burn patients who underwent outpatient follow-up was completed. Quality of life was assessed using the BSHS-B-Br, an instrument translated and validated in Brazilian Portuguese. Based on ICF category concepts, a data collection tool was used with “yes” and “no” answers. A “yes” answer represented the “8” qualifier, indicating a problem without a specific order of magnitude. Both instruments were self-applied in standardized conditions without complications during the process. Results were analyzed through Spearman’s rank correlation coefficients. The BSHS-B-Br had an average score of 127.12 (SD ± 23.03). The correlation was moderate between the total BSHS-B-Br score and the answers of ICF for body functions (r = −.53; P &lt; .001) and environmental factors (r = −.50; P &lt; .001). It was weak for body structures (r = −.47; P &lt; .001) and for activities and participation (r = −.43; P &lt; .001). This study found a moderate correlation between the results of the Burn Specific Health Scale – Brief – Brazil and the International Classification of Functioning, Disability and Health for burn patients showing that both instruments provide complementary information about burned patients.
Financial Services Trade in Special Economic Zones
Authors: Delimatsis Panagiotis 
Published: April 2021

ABSTRACT
The mushrooming of special economic zones (SEZs) in recent years has led to a significant increase in the supply of attractive incentives for investors looking for opportunities abroad. Special economic zones are self-contained regimes, inextricably associated with investment promotion policies and domestic industrial policy in general, that many times form part of a broader strategic governmental planning that aims at experimenting with economic rule-making in strictly defined territorial and jurisdictional boundaries. While empirical evidence is still inconclusive, special economic zones can contribute to economic development at the domestic level through a varying degree of channels. This is even more the case with services-only special economic zones, a new phenomenon that exemplifies the rising importance of trade in services but also the ‘servicification trend’ that drives global economic activity. Against this background, this article examines the case of services in special economic zones and offers a tour d’horizon of the current services-related special economic zone landscape. Furthermore, the article critically reviews the patterns, traits, and limits of trade in financial services within special economic zones and discusses the relevance of the General Agreement on Trade in Services at this juncture. It concludes with a discussion of potential development-related benefits ensuing from trade in financial services within special economic zones.