bluuebunny/crossref_metadata_2025_split huggingface dataset:
Glucose confers protection to Escherichia coli against contact killing by Vibrio cholerae
Authors: Crisan Cristian V. , Nichols Holly L. , Wiesenfeld Sophia , Steinbach Gabi , Yunker Peter J. , Hammer Brian K. 
Published: February 2021

AbstractEvolutionary arms races are broadly prevalent among organisms including bacteria, which have evolved defensive strategies against various attackers. A common microbial aggression mechanism is the type VI secretion system (T6SS), a contact-dependent bacterial weapon used to deliver toxic effector proteins into adjacent target cells. Sibling cells constitutively express immunity proteins that neutralize effectors. However, less is known about factors that protect non-sibling bacteria from T6SS attacks independently of cognate immunity proteins. In this study, we observe that human Escherichia coli commensal strains sensitive to T6SS attacks from Vibrio cholerae are protected when co-cultured with glucose. We confirm that glucose does not impair V. cholerae T6SS activity. Instead, we find that cells lacking the cAMP receptor protein (CRP), which regulates expression of hundreds of genes in response to glucose, survive significantly better against V. cholerae T6SS attacks even in the absence of glucose. Finally, we show that the glucose-mediated T6SS protection varies with different targets and killers. Our findings highlight the first example of an extracellular small molecule modulating a genetically controlled response for protection against T6SS attacks. This discovery may have major implications for microbial interactions during pathogen-host colonization and survival of bacteria in environmental communities.
Quantifying lumbar vertebral perfusion by a Tofts model on DCE-MRI using segmental versus aortic arterial input function
Authors: Liu Yi-Jui , Yang Hou-Ting , Yao Melissa Min-Szu , Lin Shao-Chieh , Cho Der-Yang , Shen Wu-Chung , Juan Chun-Jung , Chan Wing P. 
Published: February 2021

AbstractThe purpose of this study was to investigate the influence of arterial input function (AIF) selection on the quantification of vertebral perfusion using axial dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI). In this study, axial DCE-MRI was performed on 2 vertebrae in each of eight healthy volunteers (mean age, 36.9 years; 5 men) using a 1.5-T scanner. The pharmacokinetic parameters Ktrans, ve, and vp, derived using a Tofts model on axial DCE-MRI of the lumbar vertebrae, were evaluated using various AIFs: the population-based aortic AIF (AIF_PA), a patient-specific aortic AIF (AIF_A) and a patient-specific segmental arterial AIF (AIF_SA). Additionally, peaks and delay times were changed to simulate the effects of various AIFs on the calculation of perfusion parameters. Nonparametric analyses including the Wilcoxon signed rank test and the Kruskal–Wallis test with a Dunn–Bonferroni post hoc analysis were performed. In simulation, Ktrans and ve increased as the peak in the AIF decreased, but vp increased when delay time in the AIF increased. In humans, the estimated Ktrans and ve were significantly smaller using AIF_A compared to AIF_SA no matter the computation style (pixel-wise or region-of-interest based). Both these perfusion parameters were significantly greater using AIF_SA compared to AIF_A.
CD38 is associated with bonding-relevant cognitions and relationship satisfaction over the first 3 years of marriage
Authors: Makhanova Anastasia , McNulty James K. , Eckel Lisa A. , Nikonova Larissa , Bartz Jennifer A. , Hammock Elizabeth A. D. 
Published: February 2021

AbstractAlthough there are numerous benefits to having a satisfying romantic relationship, maintaining high levels of relationship satisfaction is difficult. Many couples experience declines in relationship satisfaction in the early years of marriage, and such declines predict not only relationship dissolution but also poor mental and physical health. Several recent studies indicate that genetic variation on the CD38 gene (CD38), at the single nucleotide polymorphism (SNP) rs3796863, is associated with cognitions and behaviors related to pair bonding; we thus leveraged longitudinal data from a sample of newlywed couples (N = 139 genotyped individuals; 71 couples) to examine whether rs3796863 is associated with relationship maintenance processes and, in turn, relationship satisfaction in the early years of marriage. Replicating and extending prior research, we found that individuals with the CC genotype (vs. AC/AA) of rs3796863 reported higher levels of gratitude, trust, and forgiveness and that trust mediated the association between rs3796863 and marital satisfaction. Moreover, the benefits conferred to CC individuals lasted over the first 3 years of marriage. To our knowledge, this is the first study to examine the link between variation inCD38rs3796863 and marital functioning over time.
Salicylic acid stabilizes Staphylococcus aureus biofilm by impairing the agr quorum-sensing system
Authors: Dotto Cristian , Lombarte Serrat Andrea , Ledesma Martín , Vay Carlos , Ehling-Schulz Monika , Sordelli Daniel O. , Grunert Tom , Buzzola Fernanda 
Published: February 2021

AbstractSalicylic acid (SAL) has recently been shown to induce biofilm formation inStaphylococcus aureusand to affect the expression of virulence factors. This study was aimed to investigate the effect of SAL on the regulatoryagrsystem and its impact onS. aureusbiofilm formation. Theagrquorum-sensing system, which is a central regulator inS. aureuspathogenicity, plays a pivotal role in the dispersal ofS. aureusmature biofilms and contributes to the creation of new colonization sites. Here, we demonstrate that SAL impairs biofilm dispersal by interfering withagrexpression. As revealed by our work, protease and surfactant molecule production is diminished, and bacterial cell autolysis is also negatively affected by SAL. Furthermore, as a consequence of SAL treatment, theS. aureusbiofilm matrix revealed the lack of extracellular DNA. In silico docking and simulation of molecular dynamics provided evidence for a potential interaction of AgrA and SAL, resulting in reduced activity of theagrsystem. In conclusion, SAL stabilized the matureS. aureusbiofilms, which may prevent bacterial cell dissemination. However, it may foster the establishment of infections locally and consequently increase bacterial persistence leading to therapeutic failure.
Diurnal and seasonal variability of CO2 and CH4 concentration in a semi-urban environment of western India
Authors: Metya Abirlal , Datye Amey , Chakraborty Supriyo , Tiwari Yogesh K. , Sarma Dipankar , Bora Abhijit , Gogoi Nirmali 
Published: February 2021

AbstractAmongst all the anthropogenically produced greenhouse gases (GHGs), carbon dioxide (CO2) and methane (CH4) are the most important, owing to their maximum contribution to the net radiative forcing of the Earth. India is undergoing rapid economic development, where fossil fuel emissions have increased drastically in the last three decades. Apart from the anthropogenic activities, the GHGs dynamics in India are governed by the biospheric process and monsoon circulation; however, these aspects are not well addressed yet. Towards this, we have measured CO2 and CH4 concentration at Sinhagad, located on the Western Ghats in peninsular India. The average concentrations of CO2 and CH4 observed during the study period are 406.05 ± 6.36 and 1.97 ± 0.07 ppm (µ ± 1σ), respectively. They also exhibit significant seasonal variabilities at this site. CH4 (CO2) attains its minimum concentration during monsoon (post-monsoon), whereas CO2 (CH4) reaches its maximum concentration during pre-monsoon (post-monsoon). CO2 poses significant diurnal variations in monsoon and post-monsoon. However, CH4 exhibits a dual-peak like pattern in pre-monsoon. The study suggests that the GHG dynamics in the western region of India are significantly influenced by monsoon circulation, especially during the summer season.
New analytic bending, buckling, and free vibration solutions of rectangular nanoplates by the symplectic superposition method
Authors: Zheng Xinran , Huang Mingqi , An Dongqi , Zhou Chao , Li Rui 
Published: February 2021

AbstractNew analytic bending, buckling, and free vibration solutions of rectangular nanoplates with combinations of clamped and simply supported edges are obtained by an up-to-date symplectic superposition method. The problems are reformulated in the Hamiltonian system and symplectic space, where the mathematical solution framework involves the construction of symplectic eigenvalue problems and symplectic eigen expansion. The analytic symplectic solutions are derived for several elaborated fundamental subproblems, the superposition of which yields the final analytic solutions. Besides Lévy-type solutions, non-Lévy-type solutions are also obtained, which cannot be achieved by conventional analytic methods. Comprehensive numerical results can provide benchmarks for other solution methods.
Dynamics of the soil respiration response to soil reclamation in a coastal wetland
Authors: Song Xiliang , Zhu Yihao , Chen Weifeng 
Published: February 2021

AbstractThe soil carbon (C) pools in coastal wetlands are known as “blue C” and have been damaged extensively owing to climate change and land reclamation. Because soil respiration (RS) is the primary mechanism through which soil carbon is released into the atmosphere at a global scale, investigating the dynamic characteristics of the soil respiration rate in reclaimed coastal wetlands is necessary to understand its important role in maintaining the global C cycle. In the present study, seasonal and diurnal changes in soil respiration were monitored in one bare wetland (CK) and two reclaimed wetlands (CT, a cotton monoculture pattern, and WM, a wheat–maize continuous cropping pattern) in the Yellow River Delta. At the diurnal scale, the RS at the three study sites displayed single-peak curves, with the lowest values occurring at midnight (00:00 a.m.) and the highest values occurring at midday (12:00 a.m.). At the seasonal scale, the mean diurnal RS of the CK, CT and WM in April was 0.24, 0.26 and 0.79 μmol CO2 m−2 s−1, and it increased to a peak in August for these areas. Bare wetland conversion to croplands significantly elevated the soil organic carbon (SOC) pool. The magnitude of the RS was significantly different at the three sites, and the yearly total amounts of CO2 efflux were 375, 513 and 944 g CO2·m−2 for the CK, CT and WM, respectively. At the three study sites, the surface soil temperature had a significant and positive relationship to the RS at both the diurnal and seasonal scales, and it accounted for 20–52% of the seasonal variation in the daytime RS. The soil water content showed a significant but negative relationship to the RS on diurnal scale only at the CK site, while it significantly increased with the RS on seasonal scale at all study sites. Although the RS showed a noticeable relationship to the combination of soil temperature and water content, the synergic effects of these two environment factors were not much higher than the individual effects. In addition, the correlation analysis showed that the RS was also influenced by the soil physico-chemical properties and that the soil total nitrogen had a closer positive relationship to the RS than the other nutrients, indicating that the soil nitrogen content plays a more important role in promoting carbon loss.
Intraspecific differences in the invasion success of the Argentine ant Linepithema humile Mayr are associated with diet breadth
Authors: Seko Yugo , Hashimoto Koya , Koba Keisuke , Hayasaka Daisuke , Sawahata Takuo 
Published: February 2021

AbstractThe Argentine ant, Linepithema humile Mayr, has spread to almost all continents. In each introduced region, L. humile often forms a single large colony (supercolony), the members of which share the haplotype “LH1”, despite the presence of other supercolonies with different genetic structures. However, the mechanisms underlying the successful invasion of LH1 ants are unclear. Here, we examined whether diet breadth differs between more successful (LH1) and less successful (LH2, LH3, LH4) L. humile supercolonies in Japan to better understand the processes responsible for invasion success. The standard ellipse areas (SEAs) of δ13C and δ15N and their ranges (CR and NR) were used as diet breadth indices. The SEAs of LH1 were much larger than those of the less successful supercolonies despite no differences in the baseline SEAs of arthropods within the supercolony habitats, indicating that the invasion success of a supercolony is associated with its diet breadth. Furthermore, LH1 had a broader CR than the other supercolonies, suggesting that which might be derived from superior resource exploitation ability. Our study highlights the importance of focusing on intraspecific differences in diet breadth among supercolonies when assessing organisms that can potentially invade and become dominant in new habitats.
Simultaneous acquisition of current and lateral force signals during AFM for characterising the piezoelectric and triboelectric effects of ZnO nanorods
Authors: Yang Yijun , Kim Kwanlae 
Published: February 2021

AbstractAtomic force microscopy (AFM) is central to investigating the piezoelectric potentials of one-dimensional nanomaterials. The AFM probe is used to deflect individual piezoelectric nanorods and to measure the resultant current. However, the torsion data of AFM probes have not been exploited to elucidate the relationship between the applied mechanical force and resultant current. In this study, the effect of the size of ZnO nanorods on the efficiency of conversion of the applied mechanical force into current was investigated by simultaneously acquiring the conductive AFM and lateral force microscopy signals. The conversion efficiency was calculated based on linear regression analysis of the scatter plot of the data. This method is suitable for determining the conversion efficiencies of all types of freestanding piezoelectric nanomaterials grown under different conditions. A pixel-wise comparison of the current and lateral force images elucidated the mechanism of current generation from dense arrays of ZnO nanorods. The current signals generated from the ZnO nanorods by the AFM probe originated from the piezoelectric and triboelectric effects. The current signals contributed by the triboelectric effect were alleviated by using an AFM probe with a smaller spring constant and reducing the normal force.
Exceptional antibacterial and cytotoxic potency of monodisperse greener AgNPs prepared under optimized pH and temperature
Authors: Riaz Muhammad , Mutreja Vishal , Sareen Shweta , Ahmad Bashir , Faheem Muhammad , Zahid Nafeesa , Jabbour Ghassan , Park Jeongwon 
Published: February 2021

AbstractIn the present work, silver nanoparticles were prepared by using the extract of Camellia Sinensis. The extract contains phytochemicals which are mainly polyphenols acting as the natural reducing and stabilizing agents leading to the formation of uniformly dispersed and stabilized silver nanoparticles. The synthesis of silver nanoparticles was significantly influenced by the impact of the pH, as well as temperature conditions. It was found that at pH 5 and 25 °C, nanoparticles of different morphologies (spherical, polygonal, capsule) and sizes were formed. However, with the increase in temperature from 25 °C to 65 °C but at the same pH, these particles started attaining the spherical shape of different sizes owing to an increase in the reduction rate. Furthermore, for the reaction of the mixture at 65 °C, an increase in pH from 5 to 11 led to an increase in the monodispersity of spherically shaped nanoparticles, attributed to the hydroxide ions facilitated reduction. The prepared nanoparticles were investigated for their antibacterial activity using Nathan’s Agar Well-Diffusion method. It was found that AgNPs prepared at pH 9 and 65 °C demonstrated strong antibacterial activity against gram-negative Escherichia coli in contrast to gram-positive Staphylococcus aureus. In reference to the cytotoxic potency, the prepared AgNPs showed clear cytotoxicity for HeLa cells and showcased a close relationship between activity and concentration as evidenced by the decrease in the percentage (100 to 30%) of metabolically active cells up to 25 µM–75 µM concentration of silver nanoparticles.
Label free, electric field mediated ultrasensitive electrochemical point-of-care device for CEA detection
Authors: Chakraborty B. , Das A. , Mandal N. , Samanta N. , Das N. , Chaudhuri C. Roy 
Published: February 2021

AbstractDeveloping point-of-care (PoC) diagnostic platforms for carcinoembryonic antigen detection is essential. However, thefew implementations of transferring the signal amplification strategies in electrochemical sensing on paper-based platforms are not satisfactory in terms of detection limit (LOD). In the quest for pushing down LOD, majority of the research has been targeted towards development of improved nanostructured substrates for entrapping more analyte molecules and augmenting the electron transfer rate to the working electrode. But, such approaches have reached saturation. This paper focuses on enhancing the mass transport of the analyte towards the sensor surface through the application of an electric field, in graphene-ZnO nanorods heterostructure. These hybrid nanostructures have been deposited on flexible polyethylene terephthalate substrates with screen printed electrodes for PoC application. The ZnO nanorods have been functionalized with aptamers and the working sensor has been integrated with smartphone interfaced indigenously developed low cost potentiostat. The performance of the system, requiring only 50 µl analyte has been evaluated using electrochemical impedance spectroscopy and validated against commercially available ELISA kit. Limit of detection of 1 fg/ml in human serum with 6.5% coefficient of variation has been demonstrated, which is more than three orders of magnitude lower than the existing attempts on PoC device.
Proteomic profiling of milk small extracellular vesicles from bovine leukemia virus-infected cattle
Authors: Rahman Md. Matiur , Takashima Shigeo , Kamatari Yuji O. , Badr Yassien , Kitamura Yuko , Shimizu Kaori , Okada Ayaka , Inoshima Yasuo 
Published: February 2021

AbstractMilk small extracellular vesicles (sEV) contain proteins that provide potential information of host physiology and immunology. Bovine leukemia virus (BLV) is an oncogenic virus that causes progressive B-cell lymphosarcoma in cattle. In this study, we aimed to explore the proteomic profile of milk sEV from BLV-infected cattle compared with those from uninfected cattle. Milk sEV were isolated from three BLV-infected and three uninfected cattle. Proteomic analysis was performed by using a comprehensive nanoLC-MS/MS method. Furthermore, gene ontology (GO) annotation and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways were used to evaluate the candidates for uniquely or differentially expressed proteins in milk sEV from BLV-infected cattle. Proteomic analysis revealed a total of 1330 common proteins in milk sEV among BLV-infected cattle, whereas 118 proteins were uniquely expressed compared with those from uninfected cattle. Twenty-six proteins in milk sEV were differentially expressed proteins more than two-fold significant difference (p < 0.05) in BLV-infected cattle. GO and KEGG analyses indicated that the candidates for uniquely or differentially expressed proteins in milk sEV had been involved in diverse biological activities including metabolic processes, cellular processes, respond to stimulus, binding, catalytic activities, cancer pathways, focal adhesion, and so on. Taken together, the present findings provided a novel insight into the proteomes of milk sEV from BLV-infected cattle.
Direct and rapid measurement of hydrogen peroxide in human blood using a microfluidic device
Authors: Gaikwad R. , Thangaraj P. R. , Sen A. K. 
Published: February 2021

AbstractThe levels of hydrogen peroxide ($${\mathrm{H}}_{2}{\mathrm{O}}_{2}$$

H
2

O
2

) in human blood is of great relevance as it has emerged as an important signalling molecule in a variety of disease states. Fast and reliable measurement of $${\mathrm{H}}_{2}{\mathrm{O}}_{2}$$

H
2

O
2

levels in the blood, however, continues to remain a challenge. Herein we report an automated method employing a microfluidic device for direct and rapid measurement of $${\mathrm{H}}_{2}{\mathrm{O}}_{2}$$

H
2

O
2

in human blood based on laser-induced fluorescence measurement. Our study delineates the critical factors that affect measurement accuracy—we found blood cells and soluble proteins significantly alter the native $${\mathrm{H}}_{2}{\mathrm{O}}_{2}$$

H
2

O
2

levels in the time interval between sample withdrawal and detection. We show that separation of blood cells and subsequent dilution of the plasma with a buffer at a ratio of 1:6 inhibits the above effect, leading to reliable measurements. We demonstrate rapid measurement of $${\mathrm{H}}_{2}{\mathrm{O}}_{2}$$

H
2

O
2

in plasma in the concentration range of 0–49 µM, offering a limit of detection of 0.05 µM, a sensitivity of 0.60 µM−1, and detection time of 15 min; the device is amenable to the real-time measurement of $${\mathrm{H}}_{2}{\mathrm{O}}_{2}$$

H
2

O
2

in the patient’s blood. Using the linear correlation obtained with known quantities of $${\mathrm{H}}_{2}{\mathrm{O}}_{2}$$

H
2

O
2

, the endogenous $${\mathrm{H}}_{2}{\mathrm{O}}_{2}$$

H
2

O
2

concentration in the blood of healthy individuals is found to be in the range of 0.8–6 µM. The availability of this device at the point of care will have relevance in understanding the role of $${\mathrm{H}}_{2}{\mathrm{O}}_{2}$$

H
2

O
2

in health and disease.
Localisation of clozapine during experimental autoimmune encephalomyelitis and its impact on dopamine and its receptors
Authors: Robichon Katharina , Sondhauss Sven , Jordan T. William , Keyzers Robert A. , Connor Bronwen , La Flamme Anne C. 
Published: February 2021

AbstractMultiple sclerosis is a disease characterised by axonal demyelination in the central nervous system (CNS). The atypical antipsychotic drug clozapine attenuates experimental autoimmune encephalomyelitis (EAE), a mouse model used to study multiple sclerosis, but the precise mechanism is unknown and could include both peripheral and CNS–mediated effects. To better understand where clozapine exerts its protective effects, we investigated the tissue distribution and localisation of clozapine using matrix-assisted laser desorption ionization imaging mass spectrometry and liquid chromatography-mass spectrometry. We found that clozapine was detectable in the brain and enriched in specific brain regions (cortex, thalamus and olfactory bulb), but the distribution was not altered by EAE. Furthermore, although not altered in other organs, clozapine levels were significantly elevated in serum during EAE. Because clozapine antagonises dopamine receptors, we analysed dopamine levels in serum and brain as well as dopamine receptor expression on brain-resident and infiltrating immune cells. While neither clozapine nor EAE significantly affected dopamine levels, we observed a significant downregulation of dopamine receptors 1 and 5 and up-regulation of dopamine receptor 2 on microglia and CD4+-infiltrating T cells during EAE. Together these findings provide insight into how neuroinflammation, as modelled by EAE, alters the distribution and downstream effects of clozapine.