bluuebunny/crossref_metadata_2025_split huggingface dataset:
CD4+ CD8αα+ T Cells in the Gastric epithelium Mediate Chronic Inflammation Induced by Helicobacter Felis
Authors: Ruan Guojing , Huang An , Hu Chupeng , Xu Ningyin , Fan Menghui , Zhang Zhenxing , Wang Yue , Xing Yingying 
Published: February 2021

Abstract
CD4+ CD8αα+ double-positive intraepithelial T lymphocytes (DP T cells), a newly characterized subset of intraepithelial T cell, has been reported to contribute to local immunosuppression. However, whether DP T cells are present in Helicobacter. pylori-induced gastritis, and their relationship with disease prognosis remain to be elucidated. In this study, We established chronic gastritis models through Helicobacter felis (H. felis) infection. Gastric infiltrating lymphocytes were isolated from H. felis-induced gastritis mice and analyzed by flow cytometry. Our results suggest that DP T cells frequency in H. felis-induced gastritis mice was higher than the uninfected mice. Gastric DP T cells derived from lamina propria cells, which distributed in the gastric epithelial layer. We found that DP T cells exhibited anti-inflammatory function. In vitro, DP T cells inhibited the maturation of dendritic cells and the proliferation of CD4+ T cell. The elimination of CD4+CD8αα+ T cells in vivo resulted in severe gastritis and a reduction of H. felis load. Additionally, vaccine with silk fibroin as delivery systems enhanced vaccine efficacy by reducing DP T cells. We demonstrated that DP T cells performed an immunosuppressive role in Helicobacter felis-induced gastritis. These findings revealed that DP T cells may affect the prognosis of the disease and the vaccine efficacy.
Redefining the Risk of Surgery for Clinical Stage IIIA (N2) Non-Small Cell Lung Cancer: A Pooled Analysis of the STS GTSD and ESTS Registry
Authors: Arndt Andrew T , Brunelli Alessandro , Cicconi Silvia , Falcoz Pierre-Emmanuel , Salati Michele , Kozower Benjamin , Liptay Michael J. , Rocco Gaetano , Karush Justin M. , Geissen Nicole , Basu Sanjib , Seder Christopher W. 
Published: February 2021

Abstract
BackgroundManagement of clinical stage IIIA-N2 (cIIIA-N2) non-small cell lung cancer (NSCLC) remains controversial. We evaluated treatment strategies and outcomes in cIIIA-N2 NSCLC patients who underwent pulmonary resection in The Society of Thoracic Surgeons General Thoracic Surgery Database (STS GTSD) and the European Society of Thoracic Surgeons (ESTS) Registry.MethodsThe STS GTSD and ESTS Registry were queried for patients who underwent pulmonary resection for cIIIA-N2 NSCLC between 2012 and 2016. Demographic variables, treatment strategies, and outcome measures were collected and analyzed. Significance of differences was determined using the X2 test for categorical variables and the Wilcoxon rank sum test for continuous variables.ResultsPulmonary resection was performed in 4279 cIIIA-N2 NSCLC patients (2928 STS GTSD; 1351 ESTS). Induction therapy was administered to 49%. Lobectomy was performed in 67.1% and pneumonectomy in 13%. Lobectomy was associated with 19.2% major morbidity and 1.6% operative mortality, while pneumonectomy was associated with 34.1% and 5%, respectively. Induction therapy was associated with a higher rate of major morbidity or mortality than upfront surgery (23.2% vs. 19.5%, p=0.004), driven by pneumonectomy (40.7% vs. 30.3%, p=0.012) rather than lobectomy (20.3% vs. 18.8%, p=0.31).ConclusionsPulmonary resection for cIIIA-N2 NSCLC is associated with low rates of operative morbidity and mortality, with lobectomy having lower morbidity and mortality than pneumonectomy. Induction therapy, particularly chemoradiotherapy, is associated with a higher rate of composite morbidity or mortality than upfront surgery in pneumonectomy patients but not lobectomy patients.
Selective Intra-Arterial 177Lu-PSMA Therapy for Castration Resistant Prostate Cancer: Preliminary Results of a Pilot Study
Authors: Sayman Haluk , Gulsen Fatih , Sager Sait , Akgun Elife , Yeyin Nami , Bilgic Seckin , Toplutas Kubra N. , Beytur Fatih , Oklu Rahmi , Aras Omer 
Published: February 2021

Abstract
Purpose177Lu-PSMA is a promising therapy for patients with metastatic castration resistant prostate cancer patients (mCRPC). To our knowledge, no study has compared the pharmacokinetic profiles of 177Lu-PSMA therapy delivered intra-arterially. We aimed to compare the feasibility and safety of selective intra-arterial (IA) administration vs conventional intravenous (IV) administration of 177Lu-PSMA in mCRPC. MethodsIn this within-patient pilot study, four patients (median age, 62.5; range, 53-72) with mCRPC who were referred to our clinic between January 2018 and September 2019 for treatment with 177Lu-PSMA. Patients were treated in two visits; in each visit receiving half of the total empiric therapeutic dose of 200 mCi, IV at the first visit and IA at the second visit. They were followed upto 8 weeks using routine parameters such as hematological status, renal function, and serum PSA levels. For each patient, organ biodistribution in lesions, kidneys, liver, bone marrow, prostate, and whole body was calculated for both administration routes according to the Medical Internal Radiation Dose (MIRD) schema. Mean absorbed doses (MAD) across all patients were compared between both administration routes. ResultsThe MAD of all metastatic lesions was significantly higher by IA vs IV administration (1.59 vs 1.20 MGy/MBq; p=0.024). The prostate gland had a lower MAD between IA and IV administrations, but the difference was not significant (0.27 vs 0.36 MGy/MBq; p=0.22). Patients tolerated IA administration very well without unexpected side effects. ConclusionThese preliminary results of an ongoing study showed that IA 177Lu-PSMA therapy is feasible and safe to treat metastatic lesions, potentially having a higher delivery efficacy compared to IV administration.This study is approved by Cerrahpasa Medical Faculty ethics committee on March 5, 2019 and retrospectively registered with file no: 83045809-604.01.02.
Visceral Adiposity Index was Independently Associated with Hyperuricemia in Patients with Polycystic Ovary Syndrome
Authors: Yun Chen , Yan Han , Hongyi Yang , Danyan Ma , Xiying Zeng , Jiawen Ye , Zheng Chen , Xin Zheng , Xiaohong Yan , Changqin Liu 
Published: February 2021

Abstract
Objective: The current study aimed to explore the prevalence rate of hyperuricemia in women with polycystic ovary syndrome (PCOS) and investigate the relationship between Visceral adiposity index (VAI) and hyperuricemia in PCOS.Methods: In this cross-sectional study, 318 PCOS women were evaluated between November 2018 to September 2020. Of them, 256 subjects with complete anthropometric and SUA data were analyzed. Multivariable linear regression and logistic regression analyses were performed to determine the associations of VAI with the SUA level and hyperuricemia.Results: The prevalence rate of hyperuricemia was 56.3% in women with PCOS and was gradually increased across tertiles of VAI, which was 2.6%, 21.3%, 22.4%, respectively. Obese subjects had significantly higher levels of systolic blood pressure (SBP), waist circumference (WC), hip circumference (HC), waist-to-hip ratio (WHR), body fat percentage (BFP), triglycerides (TG), low-density lipoprotein cholesterol (LDL-c), VAI (P<0.001) and lower high-density lipoprotein cholesterol (HDL-c) (P<0.001). Pearson correlation analysis showed the SUA level was positively correlated with BMI, BFP, WHR, log (TG), log (LDL-c), SBP, and log (VAI) and negatively correlated with HDL-c. In addition, with adjustment for potential confounding factors, multivariable linear regression and logistic regression analyses showed that VAI significantly associated with the SUA level and hyperuricemia, with the coefficient (95% confidence interval (CI)) of 9.20 (2.85-15.56, P=0.005) and the adjusted odds ratio (95% CI) of 1.32 (1.05-1.65, P=0.018), respectively.Conclusion: The present study indicates that VAI was independently associated with hyperuricemia, even with adjustment for BMI and other potential confounding factors.
ORMDL1 is Upregulated and Associated With Favorable Outcome in Colorectal Cancer
Authors: Wang Qian , Liu Wanjun , Chen Si , Luo Qianxin , Li Yichen , Peng Shaoyong , Wang Huaiming , Liu Xiaoxia , Chen Daici 
Published: February 2021

Abstract
Background: ORMDL1 gene encodes a transmembrane protein for endoplasmic reticulum and is known as crucial negative regulator for sphingolipid biogenesis. However, it has been rarely studied in tumor-related context. Therefore, its prognostic value and functional significance in colorectal cancer (CRC) remain to be explored.Methods: TCGA CRC cohort analysis, qRT-PCR, and immunohistochemistry (IHC) were used to examine the ORMDL1 expression level. The association between ORMDL1 expression and various clinical characteristics were analyzed by Chi-square tests. CRC patients’ overall survival (OS) was analyzed by Kaplan-Meier analysis. In vitro and in vivo cell-based assays were performed to explore the role of ORMDL1 in cell proliferation, invasion and migration. Transcriptional changes of cells either with ORMDL1 knockdowned or overexpressed were compared and analyzed.Results: ORMDL1 was upregulated in CRC tissues either in TCGA cohort or in our cohort. Interestingly, its expression was significantly lower in patients with metastasis compared to patients without metastasis, and high expression group had longer OS than low expression group. Knockdown of ORMDL1 expression can promote proliferation, colony formation and invasion, while attenuate migration in CRC cell lines. In opposite, forced overexpression of ORMDL1 reduced cell proliferation, colony formation and invasion, while enhanced cell migration. Epithelial-to-mesenchymal transition (EMT)-like morphologic change was induced when ORMDL1 was knockdowned. Finally, stable knockdown of ORMDL1 can promote cancer cell proliferation in vivo to some extent. Conclusion: ORMDL1 is upregulated and may serve as biomarker to predict favourable outcome in colorectal cancer.
Effects of Fly Ash Amended Soil on Biochemical Attributes and Antioxidant Responses of Medicinally Potent Plant Calendula Officinalis
Authors: Varshney Ayushi , Dahiya Praveen , Mohan Sumedha 
Published: February 2021

Abstract
Phytoremediation of fly ash (FA) deposits using metal tolerant plant species has become an important eco-friendly technique for reclamation nowadays. The present study was carried out to determine the impact of FA application on photosynthetic pigments (chlorophyll a, chlorophyll b, total chlorophyll, and carotenoids), biochemical parameters (soluble protein, reducing sugar, nitrate and nitrate reductase (NR) activity), metal accumulation (Fe, Zn, Cu, Co, Cd, Cr, and Mn) and antioxidant defense activity (SOD, CAT, POD, and APX) of Calendula officinalis. With this aim in mind, under pot culture conditions, Calendula officinalis was grown in different combinations of FA and soil which include: Control, 10%, 20%, 40%, 60%, 80%, and 100% FA. The results from the study indicated that the addition of FA (40%) in soil not only improved the physico-chemical properties of soil but also increased the photosynthetic pigment and other biochemical parameters in plants, however, these parameters declined under high FA applications. On the other hand, antioxidant enzyme activities (SOD, CAT, APX and Peroxidase) of Calendula increased with increasing FA application to combat heavy metal stress from fly ash. At high FA applications, antioxidant enzyme levels increased in leaves thereby reflecting heavy metal stress and mitigation of reactive oxygen species.
Tagasaste, Leucaena and Paulownia. Three Industrial Crops for Energy and Hemicelluloses Production
Authors: Palma Alberto , Rodriguez Javier Mauricio Loaiza , Díaz Manuel J. , García Juan Carlos , Giráldez Inmaculada , López Francisco 
Published: February 2021

Abstract
BackgroundBurning fast-growing trees for energy production can be an effective alternative to coal combustion. Thus, lignocellulosic material, which can be used to obtain chemicals with a high added value, is highly abundant, easily renewed and usually inexpensive. In this work, hemicellulose extraction by acid hydrolysis of plant biomass from three different crops (Chamaecytisus proliferus, Leucaena diversifolia and Paulownia trihybrid) was modelled and the resulting solid residues were used for energy production.ResultsThe influence of the nature of the lignocellulosic raw material and the operating conditions used to extract the hemicellulose fraction on the heat capacity and activation energy of the subsequent combustion process was examined.The heat power and the activation energy of the combustion process were found to depend markedly on the hemicellulose content of the raw material. Thus, a low content in hemicelluloses resulted in a lower increased combustion efficiency and energy yield after acid hydrolysis stage.The process was also influenced by the operating conditions of the hydrolysis treatment, which increased the superior calorific value (SCV) of the solid residue by 0.6–9.7% relative to the starting material. Also, the activation energy of combustion of the hydrolysis residues from tagasaste and paulownia was considerably lower than that for the starting materials, the difference increasing with increasing degree of conversion as well as with increasing temperature and acid concentration in the acid hydrolysis.The activation energy of combustion of the solid residues from acid hydrolysis of tagasaste and paulownia decreased markedly with increasing degree of conversion, and also with increasing temperature and acid concentration in the hydrolysis treatment. No similar trend was observed in leucaena owing to its low content in hemicelluloses.ConclusionsAcid hydrolysis of tagasaste, leucaena and paulownia provided a valorizable liquor containing a large amount of hemicelluloses and a solid residue with an increased heat power amenable to efficient valorization by combustion. There are many potential applications of the hemicelluloses-rich and lignin-rich fraction, for example as multi-components of bio-based feedstocks for 3D printing, for energy and other value-added chemicals.
Learning from Public Health and Hospital Resilience to the SARS-CoV-2 Pandemic: Protocol for a Multiple Case Study (Brazil, Canada, China, France, Japan, and Mali).
Authors: Ridde Valery , Gautier Lara , Dagenais Christian , Chabrol Fanny , Hou Renyou , Bonnet Emmanuel , David Pierre-Marie , Cloos Patrick , Duhoux Arnaud , Lucet Jean-Christophe , Traverson Lola , Oliveira Sydia Rosana de Araujo , Cazarin Gisele , Peiffer-Smadja Nathan , Touré Laurence , Coulibaly Abdourahmane , Honda Ayako , Noda Shinichiro , Tamura Toyomitsu , Baba Hiroko , Kodoi Haruka , Zinszer Kate 
Published: February 2021

Abstract
Background: All prevention efforts currently being implemented for COVID-19 are aimed at reducing the burden on strained health systems and human resources. There has been little research conducted to understand how SARS-CoV-2 has affected healthcare systems and professionals in terms of their work. Finding effective ways to share the knowledge and insight between countries, including lessons learned, is paramount to the international containment and management of the COVID-19 pandemic. The aim of this project is to compare the pandemic response to COVID-19 in Brazil, Canada, China, France, Japan, and Mali. This comparison will be used to identify strengths and weaknesses in the response, including challenges for health professionals and health systems.Methods: We will use a multiple case study approach with multiple levels of nested analysis. We chose these countries as they represent different continents and different stages of the pandemic. We will focus on several major hospitals and two public health interventions (contact tracing and testing). It is a multidisciplinary research approach that will use qualitative data through observations, document analysis, and interviews, as well as quantitative data based on disease surveillance data and other publicly available data. Given that the methodological approaches of the project are largely qualitative, the ethical risks are minimal. For the quantitative component, the data being used are publicly available.Discussion: We will deliver lessons learned based on a rigorous process and on strong evidence to enable operational-level insight for national and international stakeholders.
Esophagus Involvement in Systemic Sclerosis Ultrasound Parameters and Association with Clinical Manifestations
Authors: Ma Li , Zhu Qingli , Zhang Yan , Li Jianchu , Jiang Yuxin , Xu Dong , Zeng Xiaofeng , Hou Yong , Liu He 
Published: February 2021

Abstract
Background: The esophagus involvement in systemic sclerosis (SSc) is very common yet underestimated due to the lack of suitable evaluation tools. This study aims to explore the usefulness of ultrasound (US) in the assessment of esophagus involvement and to identify its relationship with clinical and CT manifestations.Methods: We performed esophageal US in 38 SSc patients and 38 controls. US parameters including the abdominal esophagus length, esophagus wall thickness, shear-wave elastography, gastro-esophageal (His) angle, and reflux were compared. Relationships between distinguishable US parameters and clinical/CT parameters, such as gastro-esophageal reflux disease questionnaire (GERDQ), modified Rodnan skin score (mRSS), interstitial lung disease (ILD) score, the largest esophagus diameter (Dmax), and esophagus dilation percentage (%Eop), were evaluated. Results: Abdominal esophagus length was shorter in the SSc group than the control group (2.69 cm vs 3.06 cm, P=0.018), while His angle and the angle change before and after drinking water were larger in the SSc group than the control group (121°vs 108°, P=0.000; 7.97°vs 2.92°, P=0.025). Reflux was more frequently seen in the SSc group than the control group (7/38 vs 0/38; P=0.017). As for correlation with clinical and CT parameters, His angle was higher in patients with GERDQ ≥ 8 than GERDQ < 8 (116.5°vs 125.6°, P=0.035). Patients with reflux showed higher ILD score than patients without (15.8 vs 9.6, P=0.043). Furthermore, abdominal esophagus length was negatively correlated with %Eop and Dmax (r=-0.573, P<0.001; r=-0.476, P=0.003),Conclusion: US parameters of the esophagus can distinguish SSc patients from controls, as well as had correlations with clinical and CT characteristics. Our study firstly shows that US can be used as a noninvasive and convenient method to evaluate the esophagus involvement in SSc.
Effect of Intravenous Fluid Therapy for Acute Alcohol Intoxication on Length of Time from Arrival at the Emergency Department until Awakening: A Prospective Observational Cohort Study
Authors: Terayama Takero , Sasa Ruka , Nakatani Yuka , Tanaka Fumika , Terashige Sho , Higashiyama Daishi , Sugiura Takao , Hatanaka Kosuke , Nishiyama Takashi , Takeshima Shigeto 
Published: February 2021

Abstract
Background

Acute alcohol intoxication is a common condition observed in the emergency department (ED). Intravenous fluid therapy (IVF) is often administered to treat this condition, but its effectiveness remains unclear. We therefore conducted a study to evaluate the effect of IVF on length of time from arrival at the ED until awakening, which is a novel and more appropriate variable.
Methods

This single-center, prospective observational study was conducted in the ED of Self-Defense Forces Central Hospital, a secondary emergency hospital in Tokyo from October 1, 2018 to July 31, 2019. Patients were assigned to groups to receive either a 1,000-mL bolus of lactated Ringer’s solution or only cannulation. The primary outcome was length of until awakening. Secondary outcomes were length of stay in the ED and the occurrence of conditions requiring extra care. Univariate and multivariate regression analyses were performed. Predictors of the occurrence of any event that required extra care were identified using a statistical model.
Results

The final sample included 201 patients: 109 received IVF and 92 did not. There was no significant difference in baseline characteristics between the groups. Median length of time until awakening was 211 min (interquartile range [IQR], 119 min) in the IVF group and 208 min (IQR, 149 min) in the non-IVF group (p = 0.77). Multivariate regression analysis demonstrated that the regression coefficient of IVF for length of time until awakening was − 9.55 (95% confidence interval [CI], \(-\)36.2 to 17.2). However, hemoglobin (regression coefficient, \(10.1\); 95% CI, \(0.38\)-19.9) and initial Glasgow Coma Scale score (GCS) in the ED (regression coefficient, \(-\)7.51; 95% CI, \(-\)10.8 to \(-\)4.21) were significantly associated with length of time.
Conclusions

This is the first study to evaluate the effect of IVF therapy on the length of time until awakening. IVF therapy is not effective in shortening the length of time in patients with acute alcohol intoxication in the ED. Routine administration of IVF is unnecessary in these patients. Further research is warranted to investigate who benefit from IVF therapy. A significance difference in Hb and GCS in this study also would give useful tips for the next study.
Comparison of Surgical Ligation and Endovascular Embolization in the Management of Type II Congenital Extrahepatic Portosystemic Shunt: A Retrospective Clinical Study
Authors: Zhang Jin-long , Duan Wei Dong , Fang Zhu Ting , Wang Mao Qiang , Cui Li , Bai Yan Hua , Li Xiao Hui , Du Qi Cong , Shen Meng Qiu , Duan Feng 
Published: February 2021

Abstract
Background: Surgical ligation and endovascular embolization have been recommended for type II congenital extrahepatic portosystemic shunt (CEPS); however, no consensus has been reached. This study was designed to compare the safety and efficacy of surgical ligation and endovascular embolization for the treatment of type II CEPS. Methods: In this retrospective study, 23 consecutive patients diagnosed with type II CEPS between March 2011 and April 2019 were divided into either a surgical group (n=13; 41.5±19.9years) or the interventional group (n =10; 44.9±19.7years). The surgical group underwent laparoscopic surgical ligation of the shunt alone or ligation of the shunt and splenic artery and/or vein. The interventional group underwent endovascular embolization using microcoils, detachable coils and vascular plug. Results: All 23 patients received a one-step shunt closure, and their clinical symptoms were significantly improved within 3 months post-procedure and without recurrence during follow-up. The serum ammonia levels in both groups decreased after the procedure and dropped to normal level at 6 to 12 months post-procedure. Compared with baseline, the portal vein diameter in interventional group increased significantly at 3-, 6-, 12-, and 36-month post-occlusion (P=0.01 for all). The procedure time was shorter in interventional group (127.0±43.2minutes) than surgical group (219.8±56.7minutes; P <0.001). The intraoperative blood loss in interventional group (32.0±62.5mL) was less than that in surgical group (238.5±396.9mL; P=0.001).Conclusion: Both surgical ligation and endovascular embolization are effective in the treatment of type II CEPS. Endovascular embolization has the advantages of shorter procedure time, and less intraoperative blood loss. The ligation of the portosystemic shunt and splenic artery and vein is feasible with apparent safety, and it could avoid a second surgical treatment.