bluuebunny/crossref_metadata_2025_split huggingface dataset:
Socially robotic: making useless machines
Authors: Yolgormez Ceyda , Thibodeau Joseph 
Published: August 2021

AbstractAs robots increasingly become part of our everyday lives, questions arise with regards to how to approach them and how to understand them in social contexts. The Western history of human–robot relations revolves around competition and control, which restricts our ability to relate to machines in other ways. In this study, we take a relational approach to explore different manners of socializing with robots, especially those that exceed an instrumental approach. The nonhuman subjects of this study are built to explore non-purposeful behavior, in an attempt to break away from the assumptions of utility that underlie the hegemonic human–machine interactions. This breakaway is accompanied by ‘learning to be attuned’ on the side of the human subjects, which is facilitated by continuous relations at the level of everyday life. Our paper highlights this ground for the emergence of meanings and questions that could not be subsumed by frameworks of control and domination. The research-creation projectMachine Ménagerieserves as a case study for these ideas, demonstrating a relational approach in which the designer and the machines co-constitute each other through sustained interactions, becoming attuned to one another through the performance of research.Machine Ménagerieattempts to produce affective and playful—if not unruly—nonhuman entities that invite interaction yet have no intention of serving human social or physical needs. We diverge from other social robotics research by creating machines that do not attempt to mimic human social behaviours.
Mixed variational formulations for structural topology optimization based on the phase-field approach
Authors: Marino Michele , Auricchio Ferdinando , Reali Alessandro , Rocca Elisabetta , Stefanelli Ulisse 
Published: August 2021

AbstractWe propose a variational principle combining a phase-field functional for structural topology optimization with a mixed (three-field) Hu–Washizu functional, then including directly in the formulation equilibrium, constitutive, and compatibility equations. The resulting mixed variational functional is then specialized to derive a classical topology optimization formulation (where the amount of material to be distributed is an a priori assigned quantity acting as a global constraint for the problem) as well as a novel topology optimization formulation (where the amount of material to be distributed is minimized, hence with no pre-imposed constraint for the problem). Both formulations are numerically solved by implementing a mixed finite element scheme, with the second approach avoiding the introduction of a global constraint, hence respecting the convenient local nature of the finite element discretization. Furthermore, within the proposed approach it is possible to obtain guidelines for settings proper values of phase-field-related simulation parameters and, thanks to the combined phase-field and Hu–Washizu rationale, a monolithic algorithm solution scheme can be easily adopted. An insightful and extensive numerical investigation results in a detailed convergence study and a discussion on the obtained final designs. The numerical results clearly highlight differences between the two formulations as well as advantages related to the monolithic solution strategy; numerical investigations address both two-dimensional and three-dimensional applications.
Current state of and need for enzyme engineering of 2-deoxy-D-ribose 5-phosphate aldolases and its impact
Authors: Rouvinen Juha , Andberg Martina , Pääkkönen Johan , Hakulinen Nina , Koivula Anu 
Published: August 2021

Abstract
Deoxyribose-5-phosphate aldolases (DERAs, EC 4.1.2.4) are acetaldehyde-dependent, Class I aldolases catalyzing in nature a reversible aldol reaction between an acetaldehyde donor (C2 compound) and glyceraldehyde-3-phosphate acceptor (C3 compound, C3P) to generate deoxyribose-5-phosphate (C5 compound, DR5P). DERA enzymes have been found to accept also other types of aldehydes as their donor, and in particular as acceptor molecules. Consequently, DERA enzymes can be applied in C–C bond formation reactions to produce novel compounds, thus offering a versatile biocatalytic alternative for synthesis. DERA enzymes, found in all kingdoms of life, share a common TIM barrel fold despite the low overall sequence identity. The catalytic mechanism is well-studied and involves formation of a covalent enzyme-substrate intermediate. A number of protein engineering studies to optimize substrate specificity, enzyme efficiency, and stability of DERA aldolases have been published. These have employed various engineering strategies including structure-based design, directed evolution, and recently also machine learning–guided protein engineering. For application purposes, enzyme immobilization and usage of whole cell catalysis are preferred methods as they improve the overall performance of the biocatalytic processes, including often also the stability of the enzyme. Besides single-step enzymatic reactions, DERA aldolases have also been applied in multi-enzyme cascade reactions both in vitro and in vivo. The DERA-based applications range from synthesis of commodity chemicals and flavours to more complicated and high-value pharmaceutical compounds.

Key points
• DERA aldolases are versatile biocatalysts able to make new C–C bonds.
• Synthetic utility of DERAs has been improved by protein engineering approaches.
• Computational methods are expected to speed up the future DERA engineering efforts.

Graphical abstract
Outcomes of conduction system pacing compared to right ventricular pacing as a primary strategy for treating bradyarrhythmia: systematic review and meta-analysis
Authors: Abdin Amr , Aktaa Suleman , Vukadinović Davor , Arbelo Elena , Burri Harran , Glikson Michael , Meyer Christian , Munyombwe Theresa , Nielsen Jens Cosedis , Ukena Christian , Vernooy Kevin , Gale Chris P. 
Published: August 2021

Abstract
Background
Right ventricular pacing (RVP) may cause electrical and mechanical desynchrony leading to impaired left ventricular ejection fraction (LVEF). We investigated the outcomes of RVP with His bundle pacing (HBP) and left bundle branch pacing (LBBP) for patients requiring a de novo permanent pacemaker (PPM) for bradyarrhythmia.

Methods and results
Systematic review of randomized clinical trials and observational studies comparing HBP or LBP with RVP for de novo PPM implantation between 01 January 2013 and 17 November 2020 was performed. Random and fixed effects meta-analyses of the effect of pacing technology on outcomes were performed. Study outcomes included all-cause mortality, heart failure hospitalization (HFH), LVEF, QRS duration, lead revision, atrial fibrillation, procedure parameters, and pacing metrics. Overall, 9 studies were included (6 observational, 3 randomised). HBP compared with RVP was associated with decreased HFH (risk ratio [RR] 0.68, 95% confidence interval [CI] 0.49–0.94), preservation of LVEF (mean difference [MD] 0.81, 95% CI − 1.23 to 2.85 vs. − 5.72, 95% CI − 7.64 to -3.79), increased procedure duration (MD 15.17 min, 95% CI 11.30–19.04), and increased lead revisions (RR 5.83, 95% CI 2.17–15.70, p = 0.0005). LBBP compared with RVP was associated with shorter paced QRS durations (MD 5.6 ms, 95% CI − 6.4 to 17.6) vs. (51.0 ms, 95% CI 39.2–62.9) and increased procedure duration (MD 37.78 min, 95% CI 20.04–55.51).

Conclusion
Of the limited studies published, this meta-analysis found that HBP and LBBP were superior to RVP in maintaining physiological ventricular activation as an initial pacing strategy.
Mucosal and faecal neutrophil gelatinase-associated lipocalin as potential biomarkers for collagenous colitis
Authors: Bakke Ingunn , Walaas Gunnar Andreas , Bruland Torunn , Røyset Elin Synnøve , van Beelen Granlund Atle , Escudero-Hernández Celia , Thorsvik Silje , Münch Andreas , Sandvik Arne Kristian , Østvik Ann Elisabet 
Published: August 2021

Abstract
Background
Collagenous colitis (CC) is an inflammatory bowel disease where chronic diarrhoea is the main symptom. Diagnostic markers distinguishing between CC and other causes of chronic diarrhoea remain elusive. This study explores neutrophil gelatinase-associated lipocalin (NGAL) and its mRNA lipocalin2 (LCN2) as histological and faecal disease markers in CC.

Methods
NGAL/LCN2 were studied in colonic biopsies from CC patients before and during budesonide treatment using RNA sequencing (n = 9/group), in situ hybridization (ISH) (n = 13–22/group) and immunohistochemistry (IHC) (n = 14–25/group). Faecal samples from CC (n = 3–28/group), irritable bowel syndrome diarrhoea (IBS-D) (n = 14) and healthy controls (HC) (n = 15) were assayed for NGAL and calprotectin.

Results
NGAL/LCN2 protein and mRNA expression were upregulated in active CC vs HC, and vs paired samples of treated CC in clinical remission. IHC and ISH localized increased NGAL/LCN2 mainly to epithelium of active CC, compared to almost absence in HC and treated CC. In contrast, calprotectin was solely expressed in immune cells. Despite great individual differences, faecal NGAL was significantly increased in active CC compared to HC, IBS-D and treated CC and had high test sensitivity. Faecal calprotectin levels were variably increased in active CC, but the values remained below usual clinical cut-offs.

Conclusion
NGAL/LCN2 is upregulated in the epithelium of active CC and reduced during budesonide-induced clinical remission to the level of HC and IBD-S. This was reflected in NGAL faecal concentrations. We propose NGAL as an IHC marker for disease activity in CC and a potential faecal biomarker discriminating CC from HC and IBS-D.
Rapid improvement of psychiatric stigmata after IFN-free treatment in HCV patients with and without cryoglobulinemic vasculitis
Authors: Gragnani Laura , Lorini Serena , Martini Lorenzo , Stasi Cristina , Visentini Marcella , Petraccia Luisa , Marello Niccolò , Monti Monica , Marri Silvia , Madia Francesco , Ricca Valdo , Zignego Anna Linda 
Published: August 2021

Abstract
Objective
Hepatitis C virus (HCV) causes neuropsychiatric disorders and quality of life impairment, especially in patients with cryoglobulinemic vasculitis (CV). Direct acting antivirals (DAAs) are effective in most extrahepatic HCV diseases, but limited information exists regarding the outcome of psychiatric disorders in patients with and without CV, after therapy. We aimed to evaluate psychiatric outcomes, in HCV-patients with and without CV, before and after successful DAA therapy.

Methods
We prospectively studied DAA-treated HCV-patients, stratified into presence (CV) or absence of CV (NON-CV). Four psychometric scales were administered to assess depression (HAM-D and MADRS), anxiety (HAM-A), and mania (MRS). Short-Form-36 questionnaires evaluated quality of life.

Results
Seventy-six patients were recruited, and 47 CV and 29 NON-CV were treated with antivirals. At baseline, depression and anxiety, from mild to severe, were frequently shown, with the most advanced cases in thee CV group; no patients achieved the scores for mania. A significant improvement emerged for all the psychometric scales in the entire population and in the subgroups, after viral eradication even in the short-term outcome. The Short-Form-36 summary components showed benefits.

Conclusions
After HCV eradication, the depression and anxiety scores significantly improved and severity grade generally lowered. DAA-positive effects on mental disorders should be considered part of the therapy outcome, being beneficial especially in CV patients who usually have worse baseline mental scores.

Key Points• HCV frequently causes psychiatric disorders and an often-invalidating autoimmune/lymphoproliferative disease called cryoglobulinemic vasculitis.• The new direct acting antivirals (DAAs) are very effective and well tolerated by HCV-patients.• This study shows DAA-induced benefits on depression and anxiety in HCV-patients that are especially evident in CV patients who usually have worse baseline mental scores.• DAA-induced benefits are observed in the short-term post-therapy follow-up, in contrast with data previously obtained in HCV patients treated with IFN-based anti-HCV therapy.
Cross-section instability in financial markets: impatience, extrapolation, and switching
Authors: Dieci Roberto , He Xue-Zhong 
Published: August 2021

AbstractThis paper presents a stylized model of interaction among boundedly rational heterogeneous agents in a multi-asset financial market to examine how agents’ impatience, extrapolation, and switching behaviors can affect cross-section market stability. Besides extrapolation and performance based switching between fundamental and extrapolative trading documented in single asset market, we show that a high degree of ‘impatience’ of agents who are ready to switch to more profitable trading strategy in the short run provides a further cross-section destabilizing mechanism. Though the ‘fundamental’ steady-state values, which reflect the standard present-value of the dividends, represent an unbiased equilibrium market outcome in the long run (to a certain extent), the price deviation from the fundamental price in one asset can spill-over to other assets, resulting in cross-section instability. Based on a (Neimark–Sacker) bifurcation analysis, we provide explicit conditions on how agents’ impatience, extrapolation, and switching can destabilize the market and result in a variety of short and long-run patterns for the cross-section asset price dynamics.
Inhibitory effects of ultrasound irradiation on Staphylococcus epidermidis biofilm
Authors: Koibuchi Harumi , Fujii Yasutomo , Sato’o Yusuke , Mochizuki Takashi , Yamada Toshiyuki , Cui Longzhu , Taniguchi Nobuyuki 
Published: August 2021

Abstract
Purpose
We aimed to investigate whether low-intensity continuous and pulsed wave ultrasound (US) irradiation can inhibit the formation of Staphylococcus epidermidis biofilms, for potential application in the treatment of catheter-related bloodstream infections (CRBSI).

Methods
S. epidermidis biofilms that formed on the bottom surfaces of 6-well plates were irradiated on the bottom surface using the sound cell incubator system for different intervals of time.

Results
US irradiation with continuous waves for 24 h notably inhibited biofilm formation (p < 0.01), but the same US irradiation for 12 h had no remarkable effect. Further, double US irradiation with pulsed waves for 20 min inhibited biofilm formation by 33.6%, nearly two-fold more than single US irradiation, which reduced it by 17.9%.

Conclusion
US irradiation of a lower intensity (ISATA = 6–29 mW/cm2) than used in a previous study and lower than recommended by the Food and Drug Administration shows potential for preventing CRBSI caused by bacterial biofilms.
K-80003 Inhibition of Macrophage Apoptosis and Necrotic Core Development in Atherosclerotic Vulnerable Plaques
Authors: Wang Xiaolei , Sun Zhe , Yuan Ruosen , Zhang Weifeng , Shen Yejiao , Yin Anwen , Li Yanjie , Ji Qingqi , Wang Xia , Li Yi , Zhang Min , Pan Xin , Shen Linghong , He Ben 
Published: August 2021

Abstract
Purpose
Macrophage apoptosis coupled with a defective phagocytic clearance of the apoptotic cells promotes plaque necrosis in advanced atherosclerosis, which causes acute atherothrombotic vascular disease. Nonsteroidal anti-inflammatory drug sulindac derivative K-80003 treatment was previously reported to dramatically attenuate atherosclerotic plaque progression and destabilization. However, the underlying mechanisms are not fully understood. This study aimed to determine the role of K-80003 on macrophage apoptosis and elucidate the underlying mechanism.

Methods
The mouse model of vulnerable carotid plaque in ApoE−/− mice was developed in vivo. Consequently, mice were randomly grouped into two study groups: the control group and the K-80003 group (30 mg/kg/day). Samples of carotid arteries were collected to determine atherosclerotic necrotic core area, cellular apoptosis, and oxidative stress. The effects of K-80003 on RAW264.7 macrophage apoptosis, oxidative stress, and autophagic flux were also examined in vitro.

Results
K-80003 significantly suppressed necrotic core formation and inhibited cellular apoptosis of vulnerable plaques. K-80003 can also inhibit 7-ketocholesterol-induced macrophage apoptosis in vitro. Furthermore, K-80003 inhibited intraplaque cellular apoptosis mainly through the suppression of oxidative stress, which is a key cause of advanced lesional macrophage apoptosis. Mechanistically, K-80003 prevented 7-ketocholesterol-induced impairment of autophagic flux in macrophages, evidenced by the decreased LC3II and SQSTM1/p62 expression, GFP-RFP-LC3 cancellation upon K-80003 treatment.

Conclusion
Inhibition of macrophage apoptosis and necrotic core formation by autophagy-mediated reduction of oxidative stress is one mechanism of the suppression of plaque progression and destabilization by K-80003.
The Histopathology of Severe Graded Compression in Lower Thoracic Spinal Cord Segment of Rat, Evaluated at Late Post-injury Phase
Authors: Fedorova Jana , Kellerova Erika , Bimbova Katarina , Pavel Jaroslav 
Published: August 2021

AbstractSpontaneous recovery of lost motor functions is relative fast in rodent models after inducing a very mild/moderate spinal cord injury (SCI), and this may complicate a reliable evaluation of the effectiveness of potential therapy. Therefore, a severe graded (30 g, 40 g and 50 g) weight-compression SCI at the Th9 spinal segment, involving an acute mechanical impact followed by 15 min of persistent compression, was studied in adult female Wistar rats. Functional parameters, such as spontaneous recovery of motor hind limb and bladder emptying function, and the presence of hematuria were evaluated within 28 days of the post-traumatic period. The disruption of the blood-spinal cord barrier, measured by extravasated Evans Blue dye, was examined 24 h after the SCI, when maximum permeability occurs. At the end of the survival period, the degradation of gray and white matter associated with the formation of cystic cavities, and quantitative changes of glial structural proteins, such as GFAP, and integral components of axonal architecture, such as neurofilaments and myelin basic protein, were evaluated in the lesioned area of the spinal cord. Based on these functional and histological parameters, and taking the animal’s welfare into account, the 40 g weight can be considered as an upper limit for severe traumatic injury in this compression model.
Prospective assessment of AR splice variant and PSMA detection on circulating tumor cells of mCRPC patients: preliminary analysis of patients enrolled in PRIMERA trial (NCT04188275)
Authors: Francolini G. , Loi M. , Salvestrini V. , Mangoni M. , Detti B. , Di Cataldo V. , Aquilano M. , Pinzani P. , Salvianti F. , Desideri I. , Mariotti M. , Garlatti P. , Stocchi G. , Ciccone L. P. , Lucidi S. , Salvatore G. , Sottili M. , Meattini I. , Livi L. 
Published: August 2021

AbstractIn our institution, a prospective observational trial testing micro-RNA (miRNA) and ARV7 mutational status in metastatic, castration resistant prostate cancer (mCRPC), is currently recruiting (PRIMERA trial, NCT04188275). A pre-planned interim analysis was performed when 50% of the planned accrual was reached. In this report, we explored the predictive value of Circulating Tumor Cell (CTC) detection in mCRPC patients undergoing 1st line therapy. Moreover, ARV7, ARFL, PSMA and PSA expression on CTC was reported to explore potential correlation with patient prognosis and response to therapy. PRIMERA is a prospective observational trial enrolling mCRPC patients undergoing standard treatment (ARTA + ADT) after I line ADT failure. Clinical and pathological features were collected. Outcomes selected for this preliminary analysis were time to castration resistance (TTCR), PSA at 8 weeks after ARTA therapy start, PSA drop at 8 weeks, Overall PSA drop, PSA nadir. Correlation between these outcomes and CTC detection was tested. Expression of ARV7, ARFL, PSA and PSMA was explored in CTC+ patients to assess their prevalence in this cohort and their impact on selected outcomes. Median TTCR was significantly shorter in CTC+ vs CTC− patients (32.3 vs 75 months, respectively, p = 0.03) and in ARFL+ vs ARFL− patients (30.2 vs 51.1 months, respectively, p = 0.02). ARV7, PSMA and PSA expression on CTC had no impact on median TTCR, nor on biochemical response to therapy. Patients in whom CTC and ARFL expression were detected had significant reduced TTCR. However, PSA response was not influenced by CTCs detection and specific biomarkers expression.
Economic elites and the constitutional design of sharing political power
Authors: Paniagua Victoria , Vogler Jan P. 
Published: August 2021

AbstractWhat explains the emergence and persistence of institutions aimed at preventing any ruling group from using the state apparatus to advance particularistic interests? To answer this recurring question, a burgeoning literature examines the establishment of power-sharing institutions in societies divided by ethnic or religious cleavages. Going beyond existing scholarly work focused on these specific settings, we argue that political power-sharing institutions can also be the result of common disputes within the economic elite. We propose that these institutions are likely to emerge and persist when competition between elite factions with dissimilar economic interests is balanced. To address the possibility of endogeneity between elite configurations and public institutions, we leverage natural resource diversity as an instrument for elite configurations. We show that, where geological resources are more diverse, competition between similarly powerful economic groups is more likely to emerge, leading ultimately to the establishment of power-sharing mechanisms that allow elite groups to protect their diverging economic interests.
Land Use Changes Assessment using a triangulated framework: Perception Interviews, Land-Use/Land Cover Observation, and Spatial Planning Analysis in Tanjung Batu and Derawan Island, Indonesia
Authors: Lukman Kevin Muhamad , Uchiyama Yuta , Quevedo Jay Mar D. , Harding Diana , Kohsaka Ryo 
Published: August 2021

AbstractUnderstanding land-use change is imperative in the management of Indonesian coastal ecosystems with numerous environmental impacts generated from land-use conversion. To assess these impacts in holistic perspective, we use three sources of data, land cover change, policy maps, and interviews, to triangulate the main issues. Combining these sources, we analyze land-use changes in the past based on perception interviews and secondary data from the government spatial plan policy map and observable land-cover. In Tanjung Batu and Derawan Island we found that local populations were more aware of the changes related to infrastructure development, but that there are discrepancies between assigned and actual land-use, illustrating the value of a triangulated framework.
Experiences of Students, Teachers, and Parents Participating in an Inclusive, School-Based Informal Engineering Education Program
Authors: Chen Yu-Lun , Murthi Kavitha , Martin Wendy , Vidiksis Regan , Riccio Ariana , Patten Kristie 
Published: August 2021

AbstractMany youth on the autism spectrum possess interests and strengths for STEM-related postsecondary pathways, yet there are few research-based programs to support those interests and competencies including complex problem solving and social communication. This qualitative study explored the experiences and perceived outcomes of students, teachers, and parents participating in an inclusive, strength-based, extracurricular engineering design program entitled the IDEAS Maker Club. Twenty-six students, 13 parents, and nine teachers in the program completed interviews and program logs while researchers conducted classroom observations over 2 years. Thematic analysis identified five common themes: (1) positive student experience and engagement, (2) skills acquisition, (3) development of interest in STEM and related careers, (4) social relationships and community, and (5) safe spaces that supported self-determination.