bluuebunny/crossref_metadata_2025_split huggingface dataset:
α -Lipoic Acid-Plus Ameliorates Endothelial Injury via Inhibiting the Apoptosis Pathway Mediated by Intralysosomal Cathepsins in Vivo and Vitro Endothelial Injury Model
Authors: Wang Yang , Kong Xiangqian , Bao Dejun , xu Bin , Dong Yongfei , Wei Xiangping , Niu Chaoshi 
Published: February 2021

Abstract
PurposeWe tried to explore the potential role of the α-Lipoic acid-plus (LAP) in endothelial injury in vitro and vivo models. Simultaneously, possible endovascular protective mechanisms of LAP were also investigated further. MethodsIn vitro, oxyhemoglobin (OxyHb) stimulating human umbilical vein endothelial cells (HUVECs) simulated intimal injury. In vivo, carotid artery angioplasty injury was used to generate a model of rat carotid artery intimal injury (CAII). HUVECs and rats were treated with desferrioxamine (DFO) and LAP. ResultsIn experiment 1, we found that the expressions of Cathepsin B/D in endothelial tissue increased and reached peak point in 48 hours post rat CAII. In experiment 2, firstly, the protein levels of Cathepsin B/D, cleaved-caspase-3, Bax, Ferritin, Transferrin Receptor (TfR) markedly increased after CAII and reversed by DFO and LAP treatments. Besides, DFO and LAP treatments also reduced oxidative stress level and endothelial cells (ECs) necrosis of the damaged endometrium. In experiment 3, firstly, the protein levels of Cathepsin B/D, cleaved-caspase-3, Bax, Ferritin and TfR apparently increased post OxyHb stimulation, which were further aggravated by the addition of iron and decreased by DFO and LAP treatments. Moreover, DFO and LAP significantly ameliorated oxidative stress level, HUVECs injury, iron level, mitochondrial damage and were beneficial to maintain lysosomal integrity. Finally, LAP may have exerted more significant endovascular protective effects than DFO.Conclusions﻿LAP probably exerted endovascular protective effects via inhibiting the apoptosis pathway mediated by intralysosomal Cathepsins by chelating excessive iron in endothelial lysosomes post intimal injury.
Transcriptomic Phases of Periodontitis Lesions Using the Nonhuman Primate Model
Authors: Ebersole Jeffrey , Nagarajan Radhakrishnan , Kirakodu Sreenatha , Gonzalez Octavio A. 
Published: February 2021

Abstract
We used a nonhuman primate model of ligature-induced periodontitis to identify patterns of gingival transcriptomic changes demarcating phases of periodontitis lesions (initiation, progression, resolution). 18 adult Mulatta macaca (12-22 years) had ligatures placed (premolar, 1st molar teeth) in all 4 quadrants. Gingival tissue samples were obtained (baseline, 2 weeks, 1 and 3 months during periodontitis and at 5 months resolution). Gene expression was analyzed by microarray [Rhesus Gene 1.0 ST Array (Affymetrix)]. Compared to baseline, a large array of genes were significantly altered at initiation (n=6049), early progression (n=4893), and late progression (n=5078) of disease, with the preponderance being up-regulated. Additionally, 1918 genes were altered in expression with disease resolution, skewed towards down-regulation. Assessment of the genes demonstrated specific profiles of epithelial, bone/connective tissue, apoptosis/autophagy, metabolism, regulatory, immune, and inflammatory responses that were related to health, stages of disease, and tissues with resolved lesions. Unique transcriptomic profiles occured during the kinetics of the periodontitis lesion exacerbation and remission. We delineated phase specific gene expression profiles of the disease lesion. Detection of these gene products in gingival crevicular fluid samples from human disease may contribute to a better understanding of the biological dynamics of the disease to improve patient management.
Targeting Several Biologically Reported Targets of Glioblastoma Multiforme by Assaying 2D and 3D Cultured Cells
Authors: Sixto-López Yudibeth , Marhuenda Emilie , García-Vazquez Juan Benjamin , Fragoso-Vazquez Jonathan , Rosales-Hernández Martha Cecilia , Zacarías-Lara Oscar , Méndez-Luna David , Gómez-Vidal José Antonio , Cornu David , Bakalara Norbert , Basurto Jose Correa Correa 
Published: February 2021

Abstract
Glioblastoma multiforme (GBM) is account for 70% of all primary malignancies of the central nervous system. Median The survival of human patients after treatment is around 15 months. There are However, several biological targets which have been reported that can be pursued using ligands with varied structures to treat this disease. In our group, we have developed several ligands that target a wide range of proteins involved in anticancer effects, such as histone deacetylase (HDACs), G protein-coupled estrogen receptor 1 (GPER), estrogen receptor-beta (ERβ), and NADPH oxidase (NOX), that were screened on bidimensional (2D) and tridimensional (3D) GBM stem cells like (GSC). Our results show that some HDAC inhibitors show antiproliferative properties at 21-32 µM as well as . These results suggest that in this 3D culture, HDACs could be the most relevant targets that are modulated to induce the antiproliferative effects that require in the future further experimental studies.
Levels, Daily Exposures, and Health Risk Assessment of Pesticides and Toxic Metals in Fruits of Lycium Barbarum L. (Goji berries) Sourced from Different Locations in Ningxia, China
Authors: Zhang Yahong , Qin Jiaqi , Wang Yan , Zhou Tongning , Feng Ningchuan , Ma Caihong , Zhu Meilin 
Published: February 2021

Abstract
Background: The berries of Lycium barbarum L. (Goji) are widely used as a Chinese traditional herbal medicine and functional food because of their reported beneficial pharmacological effects. However, there are reports of Goji berries being contaminated by chemical residues that could pose a hazard to humans. Methods: In this study, samples of L. barbarum L. berries were collected from plantations in a genuine production area and supermarkets in Ningxia, China. The major hazardous chemicals, including pesticides (dichlorvos, omethoate, cypermethrin, fenvalerate, malathion, and deltamethrin) and toxic metals (lead, cadmium, copper, nickel, zinc, and arsenic), were quantified by gas chromatography and inductively coupled plasma-optical emission spectrometry. In addition, associated daily exposures and health risks were determined using deterministic and probabilistic assessments. Results: The levels of five pesticides from the plantation samples were considerably lower than the maximum residue limits; only dichlorvos was detected in the supermarket samples, and deltamethrin was not detected in any samples. Copper, zinc, arsenic, lead, nickel and cadmium were detected in samples from both sources. The hazard quotient values of individual hazardous chemicals and the hazard index of combined hazardous chemicals were considerably less than 1, indicating the absence of a non-carcinogenic effect of hazardous chemical exposures through Goji berry consumption. The R value of arsenic was much less than 10-6, which shows that consumption of the Goji berries had no obvious carcinogenic risks. Conclusions: The potentially harmful effects of the L. barbarum L. are more likely from berries obtained from plantations than those from supermarkets, and heavy metal exposure is more dangerous than pesticide exposure. However, on the basis of our analysis, no population would be exposed hazardous chemicals exceeding existing standards, and the factors most affecting the health risk were exposure frequency and arsenic content.
Surgery Alone, Adjuvant Tegafur/gimeracil/octeracil (S-1), or Platinum-based Chemotherapies For Resectable Gastric Cancer: Real-world Experience and A Propensity Score Matching Analysis
Authors: Yen Chih-Chieh , Shan Yan-Shen , Chao Yin-Jui , Liao Ting-Kai , Huang Hsuan-Yi , Liu I-Ting , Yen Chia-Jui 
Published: February 2021

Abstract
Background: Adjuvant chemotherapy has changed the paradigm in resectable gastric cancer. S-1 is an oral chemotherapeutic with promising efficacy in Asia. However, comparisons with close observation or platinum-based doublets post D2 gastrectomy have been less reported.Methods: We retrospectively evaluated patients with D2-dissected stage IB-III gastric cancer who received S-1 (S-1, n=64), platinum-based doublets (P, n=95) and operation with close observation (OBS, n=110) from Jan 2013 to Oct 2018. A propensity score matching was used to compare for recurrence-free (RFS) and overall survivals (OS). Adverse reactions, dosage, and associated factors for S-1 are also discussed.Results: In a median follow-up of 42.5 months, Group S-1 had a comparable survival to that of P and OBS. (median RFS/OS: S-1 vs. P, 24.4/37.4 vs. 39.1/62.1 months, HR=1.12/1.22, p=0.667/0.514; S-1 vs. OBS, 23.6/37.4 vs. 24.0/64.6 months, HR=1.17/1.23, p=0.554/0.499). However, in patients with N2-3 or stage IIIB/IIIC disease, P significantly improved survival as compared with S-1. Conversely, S-1 was comparable to P and superior to OBS in stage IB-IIIA or N0-1 disease. S-1 was well-tolerated with a relative dose intensity of 73.6%, a median duration of 8.3 months and associated with less adverse reactions as compared with P. In the subgroup analysis, P was favored in stage III, N2-3 or T3-4 disease wherase patients with Helicobacter Pylori infection had a better survival when treated with S-1.Conclusions: Adjuvant S-1 correlated with comparable survival rates but fewer adverse reactions as compared with platinum-based doublets. Patients with low to moderate risk of recurrence and Helicobacter pylori infection derived benefits from S-1 while platinum-based doublets were favored in advanced cases. The study provides additional information about adjuvant S-1 in patients with selected risk of recurrence.
A Comprehensive Bioinformatics Analysis of TIMP2 in Multiple Malignancies
Authors: Wang Dan-Dan , Xu Wen-Xiu , Chen Wen-Quan , Yang Su-Jin , Zhang Jian , Tang Jinhai 
Published: February 2021

Abstract
Background: Tissue inhibitor of metalloproteinase-2 (TIMP2), an endogenous inhibitor of matrix metalloproteinases, has been disclosed to participate in the development and carcinogenesis of multiple malignancies. However, the prognosis of TIMP2 in different cancers and its correlation with tumor microenvironment and immunity have not been clarified.Methods: In this study, we conducted a comprehensive bioinformatics analysis to evaluate the prognostic and therapeutic value of TIMP2 in cancer patients by utilizing a series of databases, including ONCOMINE, GEPIA, cBioPortal, GeneMANIA, Metascape, and Sangerbox online tool. The expression of TIMP2 in different cancers were analyzed by Oncomine, TCGA and GTEx databases and mutation status of TIMP2 in cancers was then verified using cBioportal database. The protein-protein interaction (PPI) network of the TIMP family was exhibited by GeneMANIA. The prognosis of TIMP2 in cancers was performed though GEPIA database and cox regression. Additionally, the correlations between TIMP2 expression and immunity (immune cells, gene markers of immune cells, TMB, MSI, and neoantigen) were explored using Sangerbox online tool.Results: The transcriptional level of TIMP2 in most cancerous tissues were significantly elevated. Survival analysis revealed that elevated expression of TIMP2 was associated with unfavorable survival outcome in multiple cancers. Enrichment analysis demonstrated the possible mechanisms of TIMPs and their associated genes mainly involved in pathways including extracellular matrix (ECM) regulators, degradation of ECM and ECM disassembly, and several other signaling pathways. Conclusions: Our findings systematically dissected that TIMP2 was a potential prognostic maker in various cancers and use the inhibitor of TIMP2 may be an effective strategy for cancer therapy to improve the poor cancer survival and prognostic accuracy, but concrete mechanisms need to be validated by subsequent experiments.
Breast Cancer Incidence by Age at Finding of Mammographic Abnormality in Women Participating in French Organized Screening Campaigns.
Authors: KOÏVOGUI AKOÏ , BALAMOU CHRISTIAN , RYMZHANOVA Raushan , CORNELIS STEPHANE , RODRIGUE-MOULINIE CHRISTELLE , SELLIER NICOLAS 
Published: February 2021

Abstract
Purpose: Using reduced samples, statistical modelling was already predicted the occurrence of Breast-cancer or its prognosis from previous radiological findings. This study aims to predict breast-cancer risk by mammographic abnormalities finding age in the French breast-cancer screening campaign. Methods: The study involved 261,083 women aged 50-74 living in French Departments (Ain, Doubs, Haute-Saône, Jura, Territoire-de-Belfort, Yonne). These women had at least two screening mammograms between Jan-1999 and Dec-2017 of which the first was classified as “normal/benign”. The incidence of mammographic-abnormality (microcalcification, spiculated-mass, obscured-mass, architectural-distortion, asymmetric-density) and the incidence of breast-cancer after abnormality detection were estimated abnormalities finding age, using an actuarial life-table method. Breast-cancer risk was predicted in a Cox multivariate model.Results: The incidence of mammographic-abnormality was 95.4[94.9; 95.9]/1000 person-years. Breast-cancer (6,326 cases) incidence was 3.3[3.0; 3.1]/1000 person-years. That incidence was 5 times higher in women who showed a speculated-mass vs. those who did not (6.9[6.4; 7.4] vs. 1.3[1.2; 1.3]). Whatever the abnormality, the incidence of cancer was higher when it was present in only one breast. Depending on the spiculated-mass finding age, the risk increased by at least 40% between the age groups 55-59years (1.4[1.0; 1.8]) and ≥70years (2.4[1.9; 3.3]).Conclusion: The study showed the increased risk of cancer with the abnormalities finding age and the low risk related to the presence of the same mammographic-abnormality in both breasts compared to the isolated mammographic-abnormality in one of the breasts. This should alert radiologists to the relevance of certain diagnostic procedures in the management of a bilateral mammographic abnormality.
Correlation Between Semen Examination Based on the WHO Criteria and Sperm DNA Fragmentation Test
Authors: Okubo Tsuyoshi , Onda Noriyuki , Hayashi Teruaki , omi Kenji , Segawa Tomoya 
Published: February 2021

Abstract
Purpose: We performed and analyzed the DFI test and semen test based on the WHO criteria and compared the two using semen factors. Methods: DFI tests and general semen tests were conducted in accordance with the WHO criteria, and correlations between the two tests were investigated. The WHO criteria were set as the cutoff value for each of the following factors: fluid volume, concentration, motility, and normal morphology, and compared with the DFI results.Results: The subjects had a mean DFI of 15.6±11.5% and the DFI increased with age. In contrast, the concentration, motility, and normal morphology decreased as the DFI increased. Patients who satisfied the WHO criteria in terms of sperm concentration, motility, and normal morphology had a significantly lower DFI than the group that did not satisfy the criteria. Therefore, evaluation with a general semen test based on the WHO criteria should be regarded as a qualitative evaluation of all factors other than fluid volume.Conclusions: Male infertility due to sperm DNA fragmentation should be suspected when in vitro fertilization (IVF) results are poor despite normal semen findings based on the WHO criteria. The results of this study suggest that the DFI test may be an important evaluation in follow-up semen analyses.
Challenging collaboration of general practitioners and specialists for patients with complex chronic conditions: A scoping review to identify improvement strategies
Authors: Tomaschek Rebecca , Lampart Patricia , Scheel-Sailer Anke , Gemperli Armin , Merlo Christoph , Essig Stefan 
Published: February 2021

Abstract
BackgroundCollaboration between medical specialists and general practitioners (GPs) is well known from daily practice, but research on the topic is rather scarce. This scoping review summarizes role distributions and components of this collaboration that have potential for improvement for the care of patients with complex chronic conditions. Methods Scoping review as a knowledge synthesis for components of collaboration and role distributions between medical specialists and GPs in intervention studies. The PubMed database was searched for literature from 2010-2020.ResultsLiterature search and reference screening generated 2,174 articles. Of these, 30 articles originating from 22 unique projects were included in our synthesis. These projects focus on different patient populations and take diverse approaches to improve collaboration within an intervention. The GP is most commonly in charge of patient management and extends the scope of practice. The specialist provides support when needed. Clear definition of roles and responsibilities, resources for knowledge exchange and raising awareness through education from specialists for specific health conditions are commonly utilized interventions. Typically, combinations of process and system changes that address communication and coordination issues are applied. Most interventions improve provider and patient satisfaction, health outcomes, and reduce fragmentation of patient care.ConclusionInterventions to improve collaboration between GPs and medical specialists seem promising. Further efforts should be made to test and apply the findings in broad clinical practice.
Seizure After Cardiopulmonary bypass Surgery: A Retrospective Study
Authors: Kim Jean Hee , Jang Jinhee , Kim Hwan Wook , Kim Woojun 
Published: February 2021

Abstract
Background: We are to investigate prognostic factors of seizure after cardiopulmonary bypass surgery based on the incidence, the operation parameters, and the outcomes of seizure.Methods: Consecutive patients presented a seizure after a cardiac or aortic surgery using a cardiopulmonary bypass (CPB) during the admission between January 2006 and January 2019 were retrospectively enrolled. Patients with a previous seizure history were excluded. The medical records were reviewed on the demographics, associated medical conditions, laboratory, electroencephalography (EEG), and imaging findings, cardiopulmonary bypass parameters, types of seizure, and prognosis.Results: Among the 488 patients who received cardiopulmonary surgery using CPB, 29 patients were diagnosed with seizure during admission. There were nine cases of aortic valve replacement, six of mitral valve replacement, four of aorta graft, two of patent ductus arteriosus closure, coronary artery bypass graft, tricuspid annuloplasty, and tumor resection for each. The average durations of the operation and of cardiopulmonary bypass were 278±119 and 108±55 minutes, respectively. The average aortic cross clamping time was 72±41 minutes. The median detection time of a seizure was 34.38±83.37 hours after surgery. Twenty-three patients (79.3%) did not show recovery of mental status after surgery until the seizure was detected. Seventeen patients were diagnosed with status epilepticus, and two of them had nonconvulsive status epilepticus. Nine showed repetitive seizures and three presented a single seizure. EEG analysis showed five cases of spike-and-wave, four of periodic discharges, four of rhythmic delta, and one of burst suppression. From the image studies, sixteen patients showed stroke, two revealed posterior reversible encephalopathy syndrome, and two showed seizure related changes. Nine (31.0%) expired in 25±27 postoperative day. For the survived patients, the average duration of ICU admission was 9±7 days.Conclusions: Due to its difficulty of detecting preclinical seizure after the surgery, evaluation including performance of EEG in early postoperative phase should be considered.
Global Stability and Backward Bifurcation for a Lymphatic filariasis model
Authors: Iyare Egberanmwen Barry , Okuonghae Daniel , Osagiede Francis 
Published: February 2021

Abstract
An ODE model of Lymphatic filariasis is proposed with eight mutually disjoint compartments. The model is proven to be mathematically and epidemiologically well posed. Epidemiological interpretation of the effective reproduction number is presented. A special case is considered where the death rate due to the disease is negligible. An endemic equilibrium under this special scenario is explicitly computed and the presence of backward bifurcation under this condition is suggested. Bifurcation analysis is performed using the Castillo-Chavez and Song theorem in the special case where death due to disease is zero. When the re-infection rate is zero, backward bifurcation is shown not to be present. In such a situation, global asymptotic stability of the endemic equilibrium is established.
Clinical Effect of Partial Sensory Root Rhizotomy on Recurrence of Multiple Sclerosing Trigeminal Neuralgia After Percutaneous Balloon Compression
Authors: Li Yuanchao , Zhang Guodong , Zhang Jiaqi , Cheng Zhenguo , Lan Yanping 
Published: February 2021

Abstract
Objective To investigate the Clinical effect of partial sensory root rhizotomy(PSR) on recurrence of multiple sclerosing trigeminal neuralgia(TN-MS) after percutaneous balloon compression(PBC). Methods A retrospective analysis of the clinical data of 21 cases of recurrent multiple sclerosis trigeminal neuralgia after PBC who were treated with PSR in the Department of Neurosurgery of Xinxiang Central Hospital from January 2012 to July 2018; The diagnosis of MS was made by McDonald criteria, and using MRI to exclude REZ Regional blood vessels compress nerves; analysis of patients' preoperative and postoperative Visual Analogue Score (VAS), VAS 0-3 points means effective, 4 points and above means invalid or recurrence.Results Preoperative VAS: 8 -10 points in 21 cases, 1 day and 6 months after surgery VAS: 0-3 points in 21 cases, effective rate 100%; 12 months after surgery: 4 points in 2case, effective rate 95%, recurrence rate 5%; 18 months after surgery, 4 cases scored above 4, the effective rate was 81%, and the recurrence rate was 19%. The average point of VAS after PSR at 1 day, 6M, 12M, and 18M were lower than PBC, P<0.05, and the difference was statistically significant. The postoperative recurrence rate of PSR at 6M, 12M, and 18M is lower than that of PBC, P<0.05, and the difference is statistically significant. All 21 patients had facial sensation loss after surgery without major complications such as intracranial hemorrhage, facial palsy, and cerebrospinal fluid leakage. 1 case of intracranial infection. Conclusion PSR is effective in the treatment of TN-MS and can be used as a treatment for recurrence after PBC.