bluuebunny/crossref_metadata_2025_split huggingface dataset:
Integrated exome and RNA sequencing of TFE3-translocation renal cell carcinoma
Authors: Sun Guangxi , Chen Junru , Liang Jiayu , Yin Xiaoxue , Zhang Mengni , Yao Jin , He Ning , Armstrong Cameron , Zheng Linmao , Zhang Xingming , Zhu Sha , Sun Xiaomeng , Yang Xiaoxia , Zhao Wanbin , Liao Banghua , Pan Xiuyi , Nie Ling , Yang Ling , Chen Yuntian , Zhao Jinge , Zhang Haoran , Dai Jindong , Shen Yali , Liu Ji-Yan , Huang Rui , Liu Jiandong , Wang Zhipeng , Ni Yuchao , Wei Qiang , Li Xiang , Zhou Qiao , Haojie Huang , Liu Zhenhua , Shen Pengfei , Chen Ni , Zeng Hao 
Published: March 2021

Abstract
TFE3-translocation renal cell carcinoma (TFE3-tRCC) is a rare and heterogeneous subtype of kidney cancer that has no standard treatment for advanced disease. We described comprehensive molecular characteristics of 63 untreated primary TFE3-tRCCs based on whole-exome and RNA sequencing. TFE3-tRCC is highly heterogeneous, both clinicopathologically and genotypically. ASPSCR1-TFE3 fusion, certain fusion isoforms and high somatic copy number alteration burdens were associated with aggressive features and poor outcomes. Apart from tumors with MED15-TFE3 fusion, most TFE3-tRCCs exhibited low PD-L1 expression and low T-cell infiltration. Unsupervised transcriptomic analysis revealed five molecular clusters with distinct angiogenesis, stroma, proliferation and KRAS down signatures, which showed association with fusion patterns and prognosis. Specifically, the high angiogenesis/stroma/proliferation cluster exclusively consisted of tumors with ASPSCR1-TFE3 fusion, which was likely to benefit from combination of immune checkpoint and anti-angiogenesis inhibitors. Our findings reveal the genomic and transcriptomic features of TFE3-tRCC and provide insights into precision medicine for this disease.
Clinical experience in anti-synthetase syndrome: a monocentric retrospective analytical study
Authors: Maloir Quentin , Laurence Seidel , Christian Von Frenckell , Fanny Gester , Renaud Louis , Julien Guiot 
Published: March 2021

Abstract
OBJECTIVES
Anti-synthetase syndrome (ASS) is a rare auto-immune disorder combining autoantibodies and specifics clinical manifestations. One of the particularities of the ASS is the pleiomorphic radiological presentation seen at the initial work-up. Evaluating treatment response can also be challenging and requires a specific clinical, functional, biological and radiological monitoring. For these reasons is fundamental to identify specific radiological and clinical features of ASS to improve diagnosis and therapeutic approach.
METHODS
We retrospectively studied all patients suffering from ASS in CHU of Liège from 2008 to 2019. The diagnosis of ASS was made according to ERS's criteria. We analyzed clinical features, pulmonary function test (PFT), computed tomography (CT), and longitudinal evolution with regard to their treatments.
RESULTS
In the whole cohort of 30 patients, we identified 19 anti-JO1, 5 anti-PL12 & 6 anti-PL7. The sex-ratio is slightly in favor of male. Interestingly PL-12 syndrome was present in younger patients than those with others antibodies (mean age 39,8 vs 53,1 (JO1) & 73,3 (PL7) (p < 0.01)). 77% of the overall cohort exhibited specific pulmonary involvement without any significant difference with regards to the severity assessed by PFT at diagnosis. Whereas the radiological presentation was pleomorphic in the anti-JO1 syndrome, the anti-PL12 syndrome exhibited mainly ground glass opacities (GGO) as well as reticular abnormalities while those with anti-PL7 showed reticulations and bronchiectasis. The longitudinal CT analysis mainly showed up a reduction of consolidations and GGO with specific therapies.
CONCLUSION
In our single center retrospective study, we found different profile of autoantibodies according to age and radiological appearance.
Metabolic flux analysis of simultaneous production of vitamin B12 and propionic acid in a coupled fermentation process by Propionibacterium freudenreichii
Authors: Zhang Yuhan , Li Xiaolian , Wang Ziqiang , Wang Yunshan , Ma Yuanyuan , Su Zhiguo 
Published: March 2021

Abstract
The metabolic processes involved in simultaneous production of vitamin B12 and propionic acid by Propionibacterium freudenreichii are very complicated. To further investigate the regulatory mechanisms of this metabolism, a simplified metabolic network was established. The effects of glucose feeding, propionic acid removal, and 5,6-dimethylbenzimidazole (DMB) addition on the metabolic flux distribution were investigated. The results showed that synthesis of propionic acid can be increased by increasing the metabolic flux through the oxaloacetate and methylmalonyl-CoA branches in the early and middle stages of the coupled fermentation. After DMB addition, the synthesis of vitamin B12 was significantly enhanced via increased metabolic flux through the δ-aminolevulinate branch, which promoted the synthesis of uroporphyrinogen III, a precursor of vitamin B12. Therefore, the analysis of metabolic flux at key nodes can provide theoretical guidance for the optimization of P. freudenreichii fermentation processes. In an experimental coupled fermentation process, the concentrations of vitamin B12 and propionic acid reached 21.6 and 50.12 g/L respectively, increased by 105.71% and 73.91% compared with batch fermentation, which provides a new strategy for industrial production.
Improved hole injection/extraction using PEDOT:PSS interlayer coated onto high temperature annealed ITO electrode for efficient device performances
Authors: Dasi Gnyaneshwar , Lavanya Thyda , Sathiyan Govindasamy , Gupta Raju Kumar , Garg Ashish , Amaladass P. , KUPPUSAMY THANGARAJU 
Published: March 2021

Abstract
We demonstrate the improved device performances by using the structure of poly(3,4-ethylenedioxythiophene):poly(styrene sulfonate) (PEDOT:PSS) film coated onto the indium tin oxide (ITO) anodic electrode annealed at 400 °C under the normal ambient. The ITO thin films show the improved film quality with decreased dislocation density and lattice strain as annealing temperature increases. The spin-coated PEDOT:PSS film smoothens the wrinkle kind of surface morphology of the ITO film annealed at 400 °C. The annealed ITO (400 °C) with PEDOT:PSS interlayer improves the hole-current density in the hole-only devices (HODs) having the device structure of ITO/PEDOT:PSS/N,N'-Bis(3-methylphenyl)-N,N'-diphenylbenzidine as hole-transporting layer/Al. It enhances the efficiency of organic photovoltaic devices [ITO (annealed) /PEDOT:PSS/P3HT:PCBM (active layer)/LiF/Al] by three times higher (1.69 %) when compared to that (0.48 %) of pristine ITO based OPV device. These results show that the annealing of ITO film at the high temperature of 400 °C under the normal ambient improves the film quality and lowers the potential energy barrier at ITO/PEDOT:PSS interface for effective hole injection/extraction process, resulting in the enhanced device electrical performances.
Morphometric analysis of the His bundle (atrioventricular fascicle) in humans and other animal species, associated with electrophysiological variables. Histological and immunohistochemical study
Authors: Gómez-Torres Fabián Alejandro , SAURI AMPARO RUIZ 
Published: March 2021

Abstract
His bundle is a part of the specialized electrical conduction system that, in the normal or anormal hearts, provides connection between the atrial and ventricular myocardial compartments. The aim of this study was to perform a morphometric analysis of the characteristics of His bundle and its association with predetermined electrophysiological variables in humans, dogs, horses, and pigs. We used five hearts of the species studied. Histological sections of 5 µm thickness were obtained and stained with hematoxylin-eosin and Masson's trichrome. We also used the desmin and PAS method for precise identification of cells. His bundle was longer in horses (2.85 x 0.82 mm) and pigs (1.77 x 0.44 mm) than in dogs (1.53 x 0.26 mm) and humans, which was the shortest (1.06 x 0.23 mm). In His bundle cells, the area and diameters were significantly larger in pigs and horses than in humans (p < 0.001) and dogs (p < 0.001). We have found two patterns of organization of the components of His bundle: Group I, with large cells and a high amount of collagen fibers in ungulates (pigs and horses); group II, with smaller cells and less amount of collagen fibers in humans and dogs. Documenting differences in cell size in His bundle allows us to obtain an additional, alternative identification criterion to commonly used ones such as anatomical location. Morphological characteristics of His bundle and its cells in the different species studied coincide with rapid or slow transmission of the electrical impulse when compared with the predetermined electrophysiological variables.
Genome-wide Hierarchical Mixed Model Association Analysis
Authors: Yang Runqing , Liu Di , Hao Zhiyu , Song Yuxin , Yang Runqing , Liu Di 
Published: March 2021

Abstract
We partitioned the genomic mixed model into two hierarchies to firstly estimate genomic breeding values (GBVs) using the genomic best linear unbiased prediction and then statistically infer the association of GBVs with each SNP using the generalized least square. The genome-wide hierarchical mixed model association study (named Hi-LMM) can correct effectively confounders with polygenic effects as residuals in association tests, preventing potential false negative errors produced with GRAMMAR or EMMAX. The Hi-LMM performs the same statistical power as the exact FaST-LMM with the same computing efficiency as EMMAX. When the GBVs have been estimated precisely, Hi-LMM outperforms existing methods in statistical power, especially through joint association analysis.
Optimal cut-offs of four anthropometric measures and their predictive ability of diabetes in a nationally representative Kenyan study
Authors: Manyara Anthony Muchai 
Published: March 2021

Abstract
Background Type 2 diabetes (T2D) is one of the top non-communicable diseases in Kenya and prevention strategies are needed. Intervening to reduce obesity is the most common prevention strategy. However, black ethnicities develop T2D at lower obesity levels and it is unclear what anthropometric cut-offs could provide the best predictive ability for T2D risk. This study, therefore, aimed to determine the optimal anthropometric cut-offs and their predictive ability of T2D. Methods The study included 2159 participants (59% women) aged 35-69 years from the Kenya STEPwise survey conducted in 2014. Four anthropometric measures – body mass index (BMI), waist circumference (WC), waist to hip ratio (WHR), and waist to height ratio (WHtR) – were used. Diabetes was defined as a fasting blood glucose of ≥7.0 mmol/l or a previous diagnosis for diabetes. Optimal anthropometric cut-offs and their receiver operating characteristics, such as the area under the curve (AUC), were computed. Results Overall, the optimal cut-off for BMI, WC, WHR and WHtR were 24. 8kg.m-2, 90.0cm, 0.88 and 0.54. On disaggregation by sex, the optimal cut-off for BMI, WC, WHR and WHtR was 27.1 kg.m-2, 87cm, 0.85 and 0.55 in women and 24.8 kg.m-2, 91cm, 0.88, and 0.54 in men. Overall, WC (AUC 0.71 (95% confidence interval 0.65, 0.76)) had a better predictive ability for T2D than BMI (AUC 0.68 (0.62, 0.73), p=0.0314)). Also, WHtR (AUC 0.71 (0.66, 0.76)) had better a predictive ability for T2D than BMI (AUC 0.68 (0.62, 0.73), p=0.0182)). Conclusions WC and WHtR were better predictors of T2D than BMI and should be used for risk stratification in Kenya. A WC cut-off of 87cm in women and 91cm in men, or a WHtR cut-off of 0.55 in both men and women should be used to identify individuals at high risk of T2D.
Estimating interactions in individual participant data meta-analysis. A comparison of methods in practice.
Authors: Walker Ruth , Stewart Lesley , Simmonds Mark 
Published: March 2021

Abstract
Medical interventions may be more effective in some types of individuals than others and identifying characteristics that modify the effectiveness of an intervention is a cornerstone of precision or stratified medicine. The opportunity for detailed examination of treatment-covariate interactions can be an important driver for undertaking an individual participant data (IPD) meta-analysis, rather than a meta-analysis using aggregate data. A number of recent modelling approaches are available. We apply these methods to the Perinatal Antiplatelet Review of International Studies (PARIS) Collaboration IPD dataset and compare estimates between them. We discuss the practical implications of applying these methods, which may be of interest to aid meta-analysists in the use of these, often complex models.
Models compared included the two-stage meta-analysis of interaction terms and one-stage models which fit multiple random effects and separate within and between trial information. Models were fitted for nine covariates and five binary outcomes and results compared.
Interaction terms produced by the methods were generally consistent. We show that where data are sparse and there is low heterogeneity in the covariate distributions across trials, the meta-analysis of interactions may produce unstable estimates and have issues with convergence. In this IPD dataset, varying assumptions by using multiple random effects in one-stage models or using only within trial information made little difference to the estimates of treatment-covariate interaction. Method choice will depend on datasets characteristics and individual preference.
Morphological and molecular characters of Scutellonema brachyurus (Steiner, 1938) Andrássy, 1958 from South Africa
Authors: Shokoohi Ebrahim 
Published: March 2021

Abstract
During a survey on plant-parasitic nematodes from South Africa, Scutellonema brachyurus was recovered from soil samples collected around the rhizosphere of wild grass in the North West and Limpopo provinces. This species characterized by a hemispherical lip region with four to six annuli, basal lip’s annuli with longitudinal incisures, body length 696–904 µm (a = 25.1–33.5; b = 5.0–7.2; c = 48.9–75.3; c’ = 0.5–0.9; V = 55–60), stylet 21–27 µm length, tail rounded with 10–19 µm length and spermatheca nonfunctional and male absent. The nblast analysis based on the D2-D3 segment of 28 S rDNA placed South African populations of S. brachyurus with 98% similarity to Greece (KU059494) and 99% similarity to South African (JX472052) S. brachyurus. Besides, nblast of COI of mtDNA showed 98% similarity of the test species with South African populations of S. brachyurus (JX472096; JX472097). The phylogenetic analysis put the South African populations of S. brachyurus together with other S. brachyurus with a 100 posterior probability support. Besides, the measurements, line illustration, and scanning electron microscopy photographs are provided for S. brachyurus from South Africa.
Significance of tridymite distribution during cooling and vapor-phase alteration of ignimbrites
Authors: Heled Yuli , Rowe Michael C. , Chambefort Isabelle , Wilson Colin J.N. 
Published: March 2021

Abstract
Thick sequences of silicic ignimbrites contain complex emplacement and cooling histories, often masking contacts between ignimbrite flow packages. Mineralogical and textural variations in these sequences are primarily a function of emplacement temperature and cooling time. Here, we focus on the use of the silica polymorph tridymite to understand the association of vapor-phase crystallization and devitrification within ignimbrite flow packages. As opposed to the common occurrence of cristobalite, the restricted domains in which we observe tridymite may provide more relevant constraints for interpreting post-emplacement devitrification and vapor-phase alteration. This study examines sections through the Whakamaru (New Zealand), Bishop (U.S.A.), and Grey’s Landing (U.S.A.) ignimbrites by combining textural observations with measurements of density, groundmass crystallinity, and the distribution and proportion of tridymite to cristobalite. The rheomorphic Grey’s Landing ignimbrite represents a high-temperature end-member scenario, with widely distributed tridymite (up to 20%) resulting from a high-magmatic temperature and rapid devitrification in a low-porosity deposit. In the welded Whakamaru and Bishop ignimbrites, metastable tridymite (up to 13%) is concentrated along boundaries between flow packages. Here tridymite is interpreted to crystallize in transient permeable zones, forming during vapor-phase alteration prior to compaction, where upper denser-welded flow materials serve as vapor seals. Our results suggest that tridymite may link the initial cooling and welding history of ignimbrites to vapor-phase alteration and devitrification, and may serve as a potential mineralogical fingerprint of depositional contacts, important for consideration of lateral transport of fluids in geothermal reservoirs.
Awareness and Acceptance of Contraceptive Methods Among Women of
Reproductive Age Attending Gynae Out-Patient Clinic at a Tertiary Care
Hospital in Western Uttar Pradesh, India
Authors: Kulshrestha Kalpna , Sukla Debbarma , Verma Kalpana 
Published: March 2021

Background:
India is the second most populous country in the world. Appropriate
knowledge and usage of contraception are important.

Objective:
This cross-sectional observational study was done to assess the awareness and usage of
different methods of contraception among women of reproductive age group.

Material and Methods:
Six hundred women between the ages 18-45 years, attending Gynae OPD
at SIMS, Hapur were interviewed with the help of a predesigned questionnaire from May 2019 to
January 2020. Women having medical disorders were excluded.

Results:
Among 600 women, 52.5% were between 21 to 30 years of age. 81.2% had parity equal to
or more than two. 86% of women were of rural origin and 66.5% belonged to the Muslim community.
85.3% of women were aware of barrier contraception, 81% were aware of natural methods,
47.7% knew about IUCD, and 57.5% were aware of OCP’s. 78.2% were aware of permanent methods
of family planning. 96.3% of women were aware of at least one method of contraception. Only
37.4% of women were using contraception. There was a statistical difference among the rural and
urban populations for awareness and usage of contraception.

Conclusion:
Despite awareness of contraception, in our study population, the acceptance of contraception
was low in comparison to other studies. Fear of side effects is the most common reason for
less usage.
The Use of N-Acetyl Cysteine Versus Chromium Picolinate as an Adjuvant
to Clomiphene Citrate and Metformin in PCOS Women to Improve Ovulation
Induction and Insulin Resistance: A Pilot Randomized Controlled
Trial
Authors: Alalfy Mahmoud , Rashwan Ahmed S. S. A. , Hussein Mohamed , Bakry Ahmed , Eid Ahmed , Eid Marwa M. 
Published: March 2021

Background:
Good response to ovulation induction and in turn improving pregnancy
rates represents a big challenge in patients with PCOS.

Aim:
To assess the effectiveness of adjuvant therapy to clomiphene citrate and metformin (in the
form of N-acetyl cysteine versus Chromium Picolinate) as regards response to ovulation induction
and metabolic syndrome improvement in patients with PCOS and infertility.

Patients and methods:
This randomized controlled trial involved 108 women diagnosed with
PCOS and infertility and were divided into 3 groups; according to the treatment protocol; group 1
received clomiphene citrate plus metformin only, group2 received clomiphene citrate and metformin
plus N-acetyl cysteine and Group 3 received clomiphene citrate and metformin plus chromium
picolinate.

Results:
There was a statistically significant difference as regards good ovulation in the form of mature
follicles (≥18 mm in diameter), with the best results in group 3, followed by group 2.
Both N-acetyl cysteine and chromium picolinate, as adjuvant therapies, were more effective in reducing
the fasting insulin levels than clomiphene citrate plus metformin alone.

Conclusion:
Chromium picolinate and N-acetyl cysteine are effective adjuvant therapies for improving
ovulation, insulin resistance, and endocrinal outcomes in women with PCOS.
Mobile and Wearable Technology for the Monitoring of Diabetes-Related Parameters: Systematic Review
Authors: Rodriguez-León Ciro , Villalonga Claudia , Munoz-Torres Manuel , Ruiz Jonatan R , Banos Oresti 
Published: March 2021

Background
Diabetes mellitus is a metabolic disorder that affects hundreds of millions of people worldwide and causes several million deaths every year. Such a dramatic scenario puts some pressure on administrations, care services, and the scientific community to seek novel solutions that may help control and deal effectively with this condition and its consequences.

Objective
This study aims to review the literature on the use of modern mobile and wearable technology for monitoring parameters that condition the development or evolution of diabetes mellitus.

Methods
A systematic review of articles published between January 2010 and July 2020 was performed according to the PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-Analyses) guidelines. Manuscripts were identified through searching the databases Web of Science, Scopus, and PubMed as well as through hand searching. Manuscripts were included if they involved the measurement of diabetes-related parameters such as blood glucose level, performed physical activity, or feet condition via wearable or mobile devices. The quality of the included studies was assessed using the Newcastle-Ottawa Scale.

Results
The search yielded 1981 articles. A total of 26 publications met the eligibility criteria and were included in the review. Studies predominantly used wearable devices to monitor diabetes-related parameters. The accelerometer was by far the most used sensor, followed by the glucose monitor and heart rate monitor. Most studies applied some type of processing to the collected data, mainly consisting of statistical analysis or machine learning for activity recognition, finding associations among health outcomes, and diagnosing conditions related to diabetes. Few studies have focused on type 2 diabetes, even when this is the most prevalent type and the only preventable one. None of the studies focused on common diabetes complications. Clinical trials were fairly limited or nonexistent in most of the studies, with a common lack of detail about cohorts and case selection, comparability, and outcomes. Explicit endorsement by ethics committees or review boards was missing in most studies. Privacy or security issues were seldom addressed, and even if they were addressed, they were addressed at a rather insufficient level.

Conclusions
The use of mobile and wearable devices for the monitoring of diabetes-related parameters shows early promise. Its development can benefit patients with diabetes, health care professionals, and researchers. However, this field is still in its early stages. Future work must pay special attention to privacy and security issues, the use of new emerging sensor technologies, the combination of mobile and clinical data, and the development of validated clinical trials.
Instrumento “Aprendizajes básicos para el manejo emocional en niños y adolescentes”
Authors: Vergara-Lope Tristán Samana , Blancas Lumbreras Abigail 
Published: March 2021

El manejo emocional, con diferentes acepciones y bajo diferentes enfoques, ha adquirido importancia en los últimos años dentro de las discusiones educativas y los currículos, pero la evaluación de este tema dentro del área de la educación está aún en etapas iniciales. Como parte del proyecto Medición Independiente de Aprendizajes (MIA) se creó y validó el instrumento “Aprendizajes básicos para el manejo emocional en niños y adolescentes” (MENA). Dicho instrumento se aplicó a una muestra de 352 alumnos de primaria y secundaria de entre 6 y 16 años. Se obtuvieron 15 reactivos ordenados en cuatro factores (Afrontamiento, Control de la impulsividad, Identificación y Comprensión y expresión) que explican 49.9 % de la varianza y obtienen un alfa de Cronbach de 0.72.