bluuebunny/crossref_metadata_2025_split huggingface dataset:
Integrated analysis of the chemical-material basis and molecular mechanisms for the classic herbal formula of Lily Bulb and Rehmannia Decoction in alleviating depression
Authors: Zhang Hongxiu , Xue Xiaoyan , Pan Jin , Song Xiaobin , Chang Xing , Mao Qiancheng , Lu Yanting , Zhao Haijun , Wang Yuan , Chi Xiansu , Wang Shijun , Ma Ke 
Published: October 2021

Abstract
Background
Lily Bulb and Rehmannia Decoction (LBRD), is a traditional Chinese formula that has been shown to be safe and effective against depression; however, its material basis and pharmacological mechanisms remain unknown.

Methods
Here, ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UHPLC-Q-TOF/MS) and high-performance liquid chromatography (HPLC) were used to identify the chemical spectrum and qualitatively identify the major active ingredients in the LBRD standard decoction, respectively. Subsequently, we assessed the behavior, neuronal function and morphology, neurotransmitter levels, hypothalamic–pituitary–adrenal (HPA)-axis associated hormones, inflammatory cytokine levels, and miRNA/mRNA expression alterations in an in vitro/vivo depression model treated by the LBRD standard decoction. Finally, miRNA/mRNA regulatory networks were created through bioinformatics analysis, followed by functional experiments to verify its role in LBRD standard decoction treatment.

Results
A total of 32 prototype compounds were identified in the LBRD standard decoction, and the average quality of verbascoside in the fresh lily bulb decoction, fresh raw Rehmannia juice, and the LBRD standard decoction were 0.001264%, 0.002767%, and 0.009046% (w/w), respectively. Administration of the LBRD standard decoction ameliorated chronic unpredictable mild stress (CUMS)-induced depression-like phenotypes and protected PC12 cells against chronic corticosterone (CORT)-induced injury. The levels of neurotransmitter, cytokine, stress hormones and neuronal morphology were disrupted in the depression model, while LBRD standard decoction could work on these alterations. After LBRD standard decoction administration, four differentially expressed miRNAs, rno-miR-144-3p, rno-miR-495, rno-miR-34c-5p, and rno-miR-24-3p, and six differentially expressed mRNAs, Calml4, Ntrk2, VGAT, Gad1, Nr1d1, and Bdnf overlapped in the in vivo/vitro depression model. Among them, miR-144-3p directly mediated GABA synthesis and release by targeting Gad1 and VGAT, and miR-495 negatively regulated BDNF expression. The LBRD standard decoction can reverse the above miRNA/mRNA network-mediated GABA and BDNF expression in the in vivo/vitro depression model.

Conclusion
Collectively, the multi-components of the LBRD standard decoction altered a series of miRNAs in depression through mediating GABAergic synapse, circadian rhythm, and neurotrophic signaling pathway etc., thereby abolishing inhibitory/excitatory neurotransmitter deficits, recovering the pro-/anti-inflammatory cytokine levels and regulating the HPA-axis hormone secretion to achieve balance of the physiological function of the whole body.
Principles and biological concepts of heredity before Mendel
Authors: Poczai Péter , Santiago-Blay Jorge A. 
Published: October 2021

AbstractThe knowledge of the history of a subject stimulates understanding. As we study how other people have made scientific breakthroughs, we develop the breadth of imagination that would inspire us to make new discoveries of our own. This perspective certainly applies to the teaching of genetics as hallmarked by the pea experiments of Mendel. Common questions students have in reading Mendel’s paper for the first time is how it compares to other botanical, agricultural, and biological texts from the early and mid-nineteenth centuries; and, more precisely, how Mendel’s approach to, and terminology for debating, topics of heredity compare to those of his contemporaries? Unfortunately, textbooks are often unavailing in answering such questions. It is very common to find an introduction about heredity in genetic textbooks covering Mendel without mentions of preceding breeding experiments carried out in hisalma mater. This does not help students to understand how Mendel came to ask the questions he did, why he did, or why he planned his pea studies the way he did. Furthermore, the standard textbook “sketch” of genetics does not allow students to consider how discoveries could have been framed and inspired so differently in various parts of the world within a single historical time. In our review we provide an extended overview bridging this gap by showing how different streams of ideas lead to the eventual foundation of particulate inheritance as a scientific discipline. We close our narrative with investigations on the origins of animal and plant breeding in Central Europe prior to Mendel in Kőszeg and Brno, where vigorous debates touched on basic issues of heredity from the early eighteenth-century eventually reaching a pinnacle coining the basic questions: What is inherited and how is it passed on from one generation to another?
Salvage hypofractionated accelerated versus standard radiotherapy for the treatment of biochemical recurrence after radical prostatectomy (SHARE): the protocol of a prospective, randomized, open-label, superiority, multi-institutional trial
Authors: Park Geumju , Kim Yeon Joo , Ahn Hanjong , Park Won , Lee Ji sung , Kim Young Seok 
Published: October 2021

Abstract
Background
While several phase III trials have investigated the role of hypofractionated radiotherapy in the definitive treatment of localized prostate cancer, prospective data reporting the outcomes of hypofractionated radiotherapy in the postoperative treatment setting are sparse. Therefore, this study is designed to assess the efficacy and treatment-related toxicity of hypofractionated salvage radiotherapy for the treatment of biochemical recurrence in men who underwent radical prostatectomy. The primary objective of this trial is to investigate whether hypofractionated radiotherapy improves biochemical control compared with conventionally fractionated radiotherapy. In addition, treatment-related toxicity, quality of life, and survival will be evaluated as secondary endpoints.

Methods
In this prospective, randomized, multi-institutional trial (the SHARE study), patients with intermediate- or high-risk prostate cancer will be randomized to receive either hypofractionated radiotherapy (65 Gy in 2.5-Gy fractions) or conventionally fractionated radiotherapy (66 Gy in 2-Gy fractions). Prostate bed irradiation or elective pelvic nodal irradiation including the prostate bed will be performed using intensity-modulated radiotherapy and daily image guidance. Treatment efficacy will be assessed using the serum tumor marker prostate-specific antigen, and toxicity will be evaluated through both physician- and patient-reported outcomes. Quality of life will also be investigated.

Discussion
This study is designed to demonstrate whether hypofractionated radiotherapy is beneficial in terms of biochemical control and toxicity compared with standard salvage radiotherapy. If hypofractionated radiotherapy is shown to be superior to conventionally fractionated radiotherapy, it will mean that improved biochemical control can be achieved, accompanied by greater patient convenience and more efficient use of medical resources.

Trial registration
ClinicalTrials.gov NCT03920033. Registered on 18 April 2019
Evaluating the potential of respiratory-sinus-arrhythmia biofeedback for reducing physiological stress in adolescents with autism: study protocol for a randomized controlled trial
Authors: Thoen Anoushka , Steyaert Jean , Alaerts Kaat , Van Damme Tine 
Published: October 2021

Abstract
Background
Prior evidence points towards lower cardiac vagal modulation in individuals with autism spectrum disorder (ASD) as compared to control groups. A cross-sectional phase in this study will gather more evidence concerning this topic. A longitudinal phase will explore the efficacy of a biofeedback intervention based on respiratory sinus arrhythmia (RSA) in adolescents with ASD. Finally, a feasibility study will focus on a non-supervised RSA biofeedback intervention in this population.

Methods
The cross-sectional phase includes the comparison of adolescents with ASD (n=38) and age- and gender-matched typically developing peers. A standardized assessment will be used which contains physiological, cortisol, and behavioral measurements. The longitudinal phase contains a randomized, single-blinded, and sham-controlled design to determine the efficacy of supervised RSA biofeedback in adolescents with ASD (n=128). A follow-up phase of 5 weeks is included to evaluate the presence of retention effects. During the latter, a feasibility study will focus on a non-supervised intervention (n=64). Assessments as described previously are scheduled after the intervention and the follow-up phase.

Discussion
First, more conclusive evidence will be provided for the presence of lower cardiac vagal modulation in adolescents with ASD as well as the association between these lower values and physiological and behavioral indices. Second, the supervised intervention in adolescents with ASD is hypothesized to upregulate this cardiac vagal modulation and positively change behavioral and physiological parameters. Third, evidence regarding the feasibility and acceptability of a non-supervised intervention may open novel avenues for home-based interventions in this population.

Trial registration
ClinicalTrials.govNCT04628715. Registered on 13 November 2020.
Radiomic features of breast parenchyma: assessing differences between FOR PROCESSING and FOR PRESENTATION digital mammography
Authors: Sansone Mario , Grassi Roberta , Belfiore Maria Paola , Gatta Gianluca , Grassi Francesca , Pinto Fabio , La Casella Giorgia Viola , Fusco Roberta , Cappabianca Salvatore , Granata Vincenza , Grassi Roberto 
Published: October 2021

AbstractObjectiveTo assess the similarity and differences of radiomics features on full field digital mammography (FFDM) in FOR PROCESSING and FOR PRESENTATION data.Methods165 consecutive women who underwent FFDM were included. Breasts have been segmented into “dense” and “non-dense” area using the software LIBRA. Segmentation of both FOR PROCESSING and FOR PRESENTATION images have been evaluated by Bland–Altman, Dice index and Cohen’s kappa analysis. 74 textural features were computed: 18 features of First Order (FO), 24 features of Gray Level Co-occurrence Matrix (GLCM), 16 features of Gray Level Run Length Matrix (GLRLM) and 16 features of Gray Level Size Zone Matrix (GLSZM). Paired Wilcoxon test, Spearman’s rank correlation, intraclass correlation and canonical correlation have been used. Bilateral symmetry and percent density (PD) were also evaluated.ResultsSegmentation from FOR PROCESSING and FOR PRESENTATION gave very different results. Bilateral symmetry was higher when evaluated on features computed using FOR PROCESSING images. All features showed a positive Spearman’s correlation coefficient and many FOR-PROCESSING features were moderately or strongly correlated to their corresponding FOR-PRESENTATION counterpart. As regards the correlation analysis between PD and textural features from FOR-PRESENTATION a moderate correlation was obtained only for Gray Level Non Uniformity from GLRLM both on “dense” and “non dense” area; as regards correlation between PD and features from FOR-PROCESSING a moderate correlation was observed only for Maximal Correlation Coefficient from GLCM both on “dense” and “non dense” area.ConclusionsTexture features from FOR PROCESSING mammograms seem to be most suitable for assessing breast density.
Hepatic progenitor cells promote the repair of schistosomiasis liver injury by inhibiting IL-33 secretion in mice
Authors: Zhang Beibei , Wu Xiaoying , Li Jing , Ning An , Zhang Bo , Liu Jiahua , Song Langui , Yan Chao , Sun Xi , Zheng Kuiyang , Wu Zhongdao 
Published: October 2021

Abstract
Background
Hepatic schistosomiasis, a chronic liver injury induced by long-term Schistosoma japonicum (S. japonicum) infection, is characterized by egg granulomas and fibrotic pathology. Hepatic progenitor cells (HPCs), which are nearly absent or quiescent in normal liver, play vital roles in chronic and severe liver injury. But their role in the progression of liver injury during infection remains unknown.

Methods
In this study, the hepatic egg granulomas, fibrosis and proliferation of HPCs were analyzed in the mice model of S. japonicum infection at different infectious stages. For validating the role of HPCs in hepatic injury, tumor necrosis factor-like-weak inducer of apoptosis (TWEAK) and TWEAK blocking antibody were used to manipulate the proliferation of HPCs in wild-type and IL-33−/− mice infected with S. japonicum.

Results
We found that the proliferation of HPCs was accompanied by inflammatory granulomas and fibrosis formation. HPCs expansion promoted liver regeneration and inhibited inflammatory egg granulomas, as well as the deposition of fibrotic collagen. Interestingly, the expression of IL-33 was negatively associated with HPCs’ expansion. There were no obvious differences of liver injury caused by infection between wild-type and IL-33−/− mice with HPCs’ expansion. However, liver injury was more attenuated in IL-33−/− mice than wild-type mice when the proliferation of HPCs was inhibited by anti-TWEAK.

Conclusions
Our data uncovered a protective role of HPCs in hepatic schistosomiasis in an IL-33-dependent manner, which might provide a promising progenitor cell therapy for hepatic schistosomiasis.
Numerical solvability of generalized Bagley–Torvik fractional models under Caputo–Fabrizio derivative
Authors: Hasan Shatha , Djeddi Nadir , Al-Smadi Mohammed , Al-Omari Shrideh , Momani Shaher , Fulga Andreea 
Published: October 2021

AbstractThis paper deals with the generalized Bagley–Torvik equation based on the concept of the Caputo–Fabrizio fractional derivative using a modified reproducing kernel Hilbert space treatment. The generalized Bagley–Torvik equation is studied along with initial and boundary conditions to investigate numerical solution in the Caputo–Fabrizio sense. Regarding the generalized Bagley–Torvik equation with initial conditions, in order to have a better approach and lower cost, we reformulate the issue as a system of fractional differential equations while preserving the second type of these equations. Reproducing kernel functions are established to construct an orthogonal system used to formulate the analytical and approximate solutions of both equations in the appropriate Hilbert spaces. The feasibility of the proposed method and the effect of the novel derivative with the nonsingular kernel were verified by listing and treating several numerical examples with the required accuracy and speed. From a numerical point of view, the results obtained indicate the accuracy, efficiency, and reliability of the proposed method in solving various real life problems.
Nosocomial cluster of carbapenemase-producing Enterobacter cloacae in an intensive care unit dedicated COVID-19
Authors: Miltgen Guillaume , Garrigos Thomas , Cholley Pascal , Deleume Marine , Allou Nicolas , Allyn Jérôme , Wilkinson David A. , Lugagne Nathalie , Belmonte Olivier , Bertrand Xavier , Hocquet Didier , Mavingui Patrick 
Published: October 2021

AbstractConcomitant prevention of SARS-CoV-2 and extensively drug-resistant bacteria transmission is a difficult challenge in intensive care units dedicated to COVID-19 patients. We report a nosocomial cluster of four patients carrying NDM-1 plasmid-encoded carbapenemase-producing Enterobacter cloacae. Two main factors may have contributed to cross-transmission: misuse of gloves and absence of change of personal protective equipment, in the context of COVID-19-associated shortage. This work highlights the importance of maintaining infection control measures to prevent CPE cross-transmission despite the difficult context and that this type of outbreak can potentially involve several species of Enterobacterales.
Empirical review on the use dynamics and economics of teff in Ethiopia
Authors: Tadele Esubalew , Hibistu Tewabe 
Published: October 2021

Abstract
Background
Teff is a warm-season cereal crop and the tiniest grain on the planet. It is one of the underutilized crops that can contribute to food security and crop diversification. It is nutritious and well adapted to the growing conditions in Ethiopia, but little has been invested to expand its potential to the domestic or international markets.

Method
Comprehensive empirical review was carried out emphasizing the spatial, temporal production dynamics of teff in Ethiopia and its economic value. Different inclusion and exclusion criteria were applied and filtered pertinent to this study in country-wide verdicts.

Results
Ethiopia is not only the biggest teff-producing nation but also the only nation to have adopted teff as a staple crop. Teff contains a high nutritive value and has unique dietary benefits due to its being gluten-free and is typically preferred by health-conscious consumers. However, teff producing and value addition practice is insufficient and generally depends on conventional practices, and its marketplace is restricted local and the government imposes an export ban on it to limit the upward pressure on domestic grain prices and address local food security. Instead, other countries, such as USA, are increasingly participating in the teff market and teff has a great contribution for foreign earning through Injera. Because of its appealing nutritional and functional features, the crop’s popularity is fast growing over the world. Several health advantages have been associated with the grain, these conditions necessitate extensive investigation on the grain’s nutritional and functional qualities.

Conclusion
This study examines the use dynamics and economics of teff in Ethiopia. Teff has received restricted consideration from the global market and mainstream researches perhaps due to its orphan crop’ status. To take comparative advantage of the growing domestic and international demand of teff, intensive investment by the domestic teff industry needs to improve methods of teff producing, opening up and expanding its international market to ensuring its super global food and part of the solution to worldwide food and nutrition security gains. Ethiopia should improve to take the lead in the growing teff market and support others to engage in teff food system part.
Four magnetic resonance imaging surveillance-detected breast cancer cases in cancer-free BRCA1/2 mutation carriers
Authors: Takaoka Megumi , Ohsumi Shozo , Miyoshi Yuichiro , Takahashi Mina , Takashima Seiki , Aogi Kenjiro , Shimizu Teruhiko , Teramoto Norihiro , Yamamoto Yasuko , Okamura Miki 
Published: October 2021

Abstract
Background
Hereditary breast and ovarian cancer (HBOC) syndrome is a susceptibility syndrome for cancers, such as breast and ovarian cancer, and BRCA1/2 are its causative genes. Annual breast-enhanced magnetic resonance imaging (MRI) is recommended for BRCA1/2 mutation carriers aged over 25 years as a secondary prevention of breast cancer. However, breast MRI surveillance is rarely performed in Japan, and only four cases of breast cancer diagnosis triggered by MRI surveillance have been reported.

Case presentation
At our hospital, MRI triggered the diagnosis of breast cancer in four cancer-free BRCA1/2 mutation carriers. In one of our four cases, although MRI showed only a 3-mm focus, we could diagnose breast cancer by shortening the surveillance interval considering the patient’s high-risk for developing breast cancer.

Conclusions
Image-guided biopsy, including MRI-guided biopsy, depending on the size of the lesion, and shorter surveillance intervals are useful when there are potentially malignant findings on breast MRI surveillance for cancer-free patients with HBOC.
The association between problematic Internet use, eating disorder behaviors, and well-being among Palestinian university students
Authors: Mahmid Fayez , Bdier Dana , Chou Priscilla 
Published: October 2021

AbstractObjectivesThe current study aimed to test the correlation between problematic Internet use, eating disorder behaviors, and well-being among Palestinian university students.MethodsTo examine the relationship between the study variables, a correlational study was conducted. The geographical representation of the study sample showed that 48.1% of participants were from urban populations, 48.1% were from rural villages, and 3.8% were from internally displaced people’s camps.ResultsPearson’s correlation coefficient was used to test the relationship between problematic Internet use, eating disorder behaviors, and well-being. Results showed that problematic Internet use was negatively correlated to well-being (r =− .32, p < .01), and positively correlated to eating disorder behaviors(r = .39,p <.01). The regression analysis found that problematic Internet use contributes statistically and significantly towards explaining variance in eating disorder behaviors (B = .46, SE= .08,β= .32). Moreover, well-being contributed in a way that was statistically significant towards explaining variance in eating disorders behaviors (B =− .39, SE= .09,β= − .25).ConclusionThe results of our study support previous studies that indicated that problematic Internet use was significantly and positively correlated with eating disorder behaviors, while it was significantly and negatively correlated to well-being among Palestinian university students. Further studies testing this relationship will be crucial in developing interventions to both reduce problematic Internet use and eating disorder behaviors and increase well-being among university students.
Gender difference in the effect of cultural distance on academic performance among cross-border students in China
Authors: Hu Jieyi , Cheung Chau Kiu 
Published: October 2021

AbstractCross-border students’ academic performance draws people’s attention, whereas perceived cultural distance might influence their performance with gender difference. Based on role theory, men and women present different roles in society, and women are good at perceptual, cognitive aspects, making them more sensitive to cultural distance. Finding shows that the negative moderation role of gender existed in the relationship between cultural distance and academic performance. Particularly, female students showed lower cultural adaptation after cross-border migration, which then influenced their academic performance in universities. This study provides implication for policymakers and universities to pay more attention with additional resources to support female students’ cultural adaption.