bluuebunny/crossref_metadata_2025_split huggingface dataset:
Assessment of salivary matrix metalloproteinase (MMP8) and activated salivary matrix metalloproteinase (aMMP8) in periodontitis patients: a systematic review and meta-analysis
Authors: Boynes Sean G. , Sofiyeva Nigar , Saw Tina , Nieto Valerie , Palomo Leena 
Published: February 2025

IntroductionPeriodontitis affects a significant portion of the global population and is associated with systemic health issues. Salivary biomarkers such as salivary matrix metalloproteinase-8 (MMP-8) and its activated form (aMMP-8) have been studied for their roles in tissue degradation and inflammation in periodontitis. This meta-analysis investigates the association between salivary MMP-8 and aMMP-8 levels and periodontitis.MethodsA systematic literature search was conducted utilizing PubMed, Embase, Web of Science, and Cochrane Library databases up to October 2023, yielding 35 studies that quantified MMP-8 or aMMP-8 in saliva from patients with periodontitis and healthy controls. Data were extracted, and standardized mean differences (SMD) with 95% confidence intervals (CI) were calculated. Heterogeneity was assessed, and subgroup analyses were performed based on saliva collection techniques. Meta-regression analysis evaluated the impact of publication year on heterogeneity.ResultsThe meta-analysis included 35 studies. Pooled results indicated significantly higher levels of MMP-8 and aMMP-8 in periodontitis cases compared to healthy controls (SMD: 2.71, 95% CI: 1.04–4.38, p = 0.002) with substantial heterogeneity (I2 = 94.5%). No significant difference was found between MMP-8 and aMMP-8 (p = 0.445). Subgroup analyses by saliva collection technique did not reduce heterogeneity significantly. Meta-regression showed that publication year did not impact heterogeneity. Small-study effects and publication bias were present, suggesting caution in interpreting the results.DiscussionThe findings support the potential of MMP-8 and aMMP-8 as biomarkers for periodontitis, although substantial heterogeneity and methodological differences among studies pose challenges. Standardized protocols and larger sample sizes are necessary to enhance the reliability of these biomarkers in clinical practice. Despite limitations, salivary diagnostics hold promise for non-invasive, early detection and monitoring of periodontitis.ConclusionSalivary MMP-8 and aMMP-8 levels are significantly associated with periodontitis, highlighting their potential as diagnostic biomarkers. However, methodological improvements and standardization are essential for their clinical application. Collaborative efforts and advancements in salivary diagnostics are crucial for improving periodontitis management and patient outcomes.
Vortex fluidic enhanced enzymatic hydrolysis of gelatin from barramundi skin for 3D printing
Authors: Sun Xiaoqi , Wu Yixiao , Wang Hao , He Shan , Young David J. , Thennadil Suresh , Raston Colin L. , Abukhadra Mostafa R. , El-Sherbeeny Ahmed M. , Deng Shanggui , Jellicoe Matt 
Published: February 2025

IntroductionProcessing with a continuous flow thin film vortex fluidic device (VFD) significantly improves the efficiency of enzymatic hydrolysis of barramundi skin gelatin compared with conventional methodology.MethodsDegree of hydrolysis, scanning electron microscopy, rheological properties, texture profile analysis, and dynamic light scattering were applied in this study.Results and discussionThe processing time was reduced from 120 min to 20 min, and the degree of hydrolysis increased from 55.0 to 74.5%. VFD-treated gelatin hydrolysates were combined with starch in different proportions for use as 3D printing inks. The ink composed of 60% starch and 40% fish gelatin hydrolyate gave an ink with a regular crosslinked internal structure, relatively high storage modulus (G’), adhesiveness (399 g.sec) and loss modulus (G”) suitable for 3D printing. This new, one-step processing methodology has the potential to add value to an abundant waste product of the seafood industry.
Research trends and hotspots of Colles fracture: a bibliometric analysis from 1980 to 2023
Authors: Zhou Chaoxi , Yu Guangrong , Wang Qinglei 
Published: February 2025

BackgroundColles fractures, a common type of distal radius fracture, predominantly affect older adults and are often associated with osteoporosis. Understanding the epidemiology, treatment methods, and complications of Colles fractures is crucial for improving patient outcomes.ObjectiveThis bibliometric analysis aims to assess the trends, influential research, and collaboration patterns in Colles fracture studies from 1980 to 2023, providing insights into emerging areas of research.MethodsLiterature was retrieved from the Web of Science Core Collection (WoSCC), Science Citation Index Expanded (SCI-EXPANDED) using the search term “Colles fracture”. A total of 948 relevant documents, including 901 articles and 47 reviews, were analyzed. VOSviewer, CiteSpace, and bibliometrix were utilized for visualization and data analysis, focusing on publication trends and hotspots.ResultsThe analysis revealed a steady increase in publications and citation counts, peaking around 2010, with a notable decline in publication output post-2010 while citations continued to rise. The USA led in both publication volume and citation impact, with significant contributions from England, Canada, Germany, and Japan. Key authors such as Cooney WP and Jupiter JB were identified as influential, while the Journal of Hand Surgery-American Volume emerged as the leading publication outlet. Keyword analysis indicated a growing emphasis on epidemiology and outcomes research, reflecting broader public health concerns.ConclusionThis bibliometric analysis highlights the evolving research on Colles fractures from 1980 to 2023. Despite a plateau in publication rates, citations continue to increase, indicating the lasting influence of earlier studies. Significant advancements have been made in treatment methods, particularly in external fixation (EF) and open reduction internal fixation (ORIF). The growing interdisciplinary focus on Colles fractures, osteoporosis, and rehabilitation underscores the need for continued research to enhance clinical outcomes and preventive measures.
Evaluating the role of nursing interventions in enhanced recovery after surgery for minimally invasive spine surgery: a retrospective analysis
Authors: Zhang Dan , Ding Hongmei , Shen Caiping , Liu Yanyan , Jia Nan 
Published: February 2025

BackgroundEnhanced Recovery After Surgery (ERAS) protocols have revolutionized postoperative care, particularly in minimally invasive spine surgery (MISS). This study aims to evaluate the role of nursing interventions in improving patient outcomes and reducing healthcare costs within this framework.MethodsThis retrospective cohort study evaluated 150 patients undergoing MISS at The First Affiliated Hospital of Guangzhou University of Chinese Medicine from January 2017 to December 2021. Of these, 75 were assigned to the conventional group and 75 to the ERAS group. The study compared conventional nursing care with the ERAS protocol, assessing clinical outcomes and hospital expenses.ResultsThe analysis revealed that implementing targeted nursing interventions significantly decreased the length of hospital stay (LOS) in the ERAS group compared to the conventional group (3.2 days vs. 4 days; p &lt; 0.001). Moreover, the multivariate analysis demonstrated that the patients in the the conventional group had significantly higher odds of prolonged length of stay (LOS) as compared to the ERAS group (OR: 5.114; 95% CI: 2.345–11.152, p &lt; 0.001). Furthermore, postoperative drainage volumes were markedly lower in the ERAS group than in the conventional cohort (p &lt; 0.001). Opioid consumption was also reduced, with only 24% of patients in the ERAS group requiring opioids, compared to 45.3% in the conventional care group (p = 0.01). Additionally, the ERAS protocol resulted in lower overall hospital expenses, highlighting its cost-effectiveness in enhancing patient outcomes.ConclusionThe implementation of targeted nursing interventions within the ERAS protocol significantly improves patient outcomes in MISS. The ERAS group demonstrated shorter hospital stays, reduced postoperative drainage, and lower opioid requirements compared to the conventional care group. Additionally, the cost-effectiveness of the ERAS protocol highlights its potential to enhance overall healthcare efficiency.
Transvaginal natural orifice transluminal endoscopic surgery for early-stage ovarian cancer and borderline ovarian tumors: a case series
Authors: Kellerhals Gaétan , Nef James , Hurni Yannick , Huber Daniela 
Published: February 2025

IntroductionSurgery is the cornerstone of ovarian cancer treatment. Transvaginal natural orifice transluminal endoscopic surgery (vNOTES) is a novel, minimally invasive technique that is gaining interest in gynecological oncology. However, its use in ovarian cancer is still limited, with only a few cases reported. This study aimed to evaluate the feasibility of vNOTES for the surgical staging of borderline and early-stage ovarian cancer.MethodsWe retrospectively reviewed all cases of borderline ovarian tumors (BOTs) and early-stage ovarian cancer surgically staged by vNOTES at our institution between October 2021 and August 2024.ResultsEleven patients were included, seven with early-stage ovarian or tubal cancer and 4 with BOTs. The median age was 47 (27–81) years, and the median body mass index was 28.1 (22.4–39.2) kg/m2. Complete vNOTES staging was achieved in all cases, including peritoneal washing, unilateral/bilateral salpingo-oophorectomy, abdominal cavity inspection, peritoneal biopsies, infracolic omentectomy, and total hysterectomy when required. The median operating time was 70 (35–138) min, with a median blood loss of 50 (10–100) ml. No intraoperative complications occurred except for one case of minor ovarian spillage. No conversions to conventional laparoscopy or laparotomy were needed. Postoperative complications included one surgical site infection (9.1%) and 2 cases of postoperative cystitis (18.2%). No severe complications graded ≥3 on the Clavien-Dindo classification were observed.ConclusionvNOTES appears to be a feasible approach for the surgical staging of highly selected patients with early-stage adnexal malignancies. Further studies are needed to validate its long-term safety and oncological outcomes.
Comparing mid-term outcomes and patient satisfaction between percutaneous endoscopic lumbar discectomy and microendoscopic discectomy for foraminal and extraforaminal lumbar disc herniations: a retrospective matched cohort study
Authors: Liu Sen , Zhao Feng , Yin Chun-Ping , Zhu Chao-Hua , Zhao Ruo-Yu , Liu Guo-Bin , Ji Gang , Chen Jia , Gao Hong-Yang 
Published: February 2025

ObjectiveThis study aimed to compare the mid-term outcomes and patient satisfaction between percutaneous endoscopic lumbar discectomy (PELD) and microendoscopic discectomy (MED) for the treatment of foraminal and extraforaminal lumbar disc herniations.MethodsA retrospective matched cohort study was conducted, including patients diagnosed with foraminal or extraforaminal lumbar disc herniations who underwent PELD or MED between January 2014 and December 2021. Patient demographics, clinical characteristics, and perioperative data were analyzed. Primary outcomes included Visual Analog Scale (VAS) scores for pain, Japanese Orthopaedic Association (JOA) scores and improvement rates for functional status, and overall satisfaction at a minimum 2-year follow-up.ResultsA total of 133 patients were included in the final analysis. The PELD group demonstrated a significantly greater reduction in VAS scores for low back pain (6.74 ± 1.21 to 1.95 ± 0.42) compared to the MED group (6.93 ± 1.17 to 2.35 ± 0.89) at the 2-year follow-up (p &lt; 0.001). Both groups exhibited significant improvements in JOA scores, with no notable differences observed at the final follow-up. Patient satisfaction rates were higher in the PELD group, with 86% reporting high satisfaction compared to 72% in the MED group; however, this difference was not statistically significant. Logistic regression analysis identified VAS scores for low back pain, operation cost, and symptom recurrence as independent factors influencing patient dissatisfaction at 2 years post-surgery.ConclusionBoth PELD and MED demonstrated efficacy in treating foraminal and extraforaminal lumbar disc herniations over a 2-year follow-up period. PELD, however, exhibited superior relief of low back pain. Factors, such as low back pain intensity, surgical costs, and symptom recurrence significantly impacted patient dissatisfaction, despite comparable overall satisfaction rates between the two surgical techniques.
Metabolic diversity and responses of anteater clostridial isolates to chitin-based substrates
Authors: Amin Ahmad , Modrackova Nikol , Tejnecky Vaclav , Neuzil-Bunesova Vera 
Published: February 2025

AimClostridium species, such as Clostridium perfringens, C. baratii, C. colicanis, Paraclostridium bifermentans, and Paeniclostridium sordellii, are Gram-positive, anaerobic, endospore-forming bacteria with diverse pathogenic mechanisms. While these species are commensals in the guts of variable animal species, such as anteaters, they are less frequently found in humans. The diet of anteaters, which includes chitin and formic acid, plays an important role in their specific dietary habits, as well as in clostridial metabolism.Methods and resultsThis study investigates the metabolic diversity and responses of anteater clostridial isolates to various substrates, namely chitin, chitosan, cellulose, N-acetyl-D-glucosamine (NAG), and glucose. All tested clostridia were able to grow in the presence of chitin, cellulose, NAG, and glucose, but varied in metabolite production. However, the presence of chitosan surprisingly showed an antimicrobial effect against clostridia, especially Pae. sordellii, P. bifermentans, and C. colicanis. The results demonstrate significant variations in fermentation profiles, and metabolite production across substrates and clostridial species. Acetate production was detected as common for all tested clostridia despite species variability and incoming substrates, as well as lactate, butyrate, propionate, and formate for some strains.ConclusionIn relation to digestion, anteater clostridia could play an important role in chitin and its degradation products, which, in the end, can influence clostridial occurrence and pathogenicity via chitosan.
Shrub Control by Burning and Clearing in the Southern Pyrenees: Effects on Soils After Two Years of Treatment
Authors: Quintana-Esteras Silvia , Badía-Villas David , Martí-Dalmau Clara 
Published: February 2025

Prescribed burns and selective shrub clearing are widely implemented as management strategies to stop the shrub encroachment of grasslands, decrease fuel loads and fire risks, and improve biodiversity and ecosystem functionality in mountain environments. While the short-term effects of burns on soil have been extensively studied, the impact of mechanical treatments on soil has received comparatively less attention. This study aims to: i) evaluate the physical, chemical, and biological characteristics of subalpine soils influenced by prescribed burns and selective clearing, and ii) assess the effectiveness of these interventions by examining the changes in vegetation cover 2 years after implementation. The research was conducted in the Central Pyrenees, where three plots were selected according to their management type: a prescribed burn plot (B), a clearing plot (CL), and a shrubland control plot (C). The results highlight how both treatments increased soil pH and reduced other properties (EC, BD, moisture, GLU) after 2 years of study, with burned and cleared plots showing similar trends in all cases. The carbon source utilization patterns of soil microbial communities (CLPP) remained unchanged by either treatment, which may indicate the short-term resilience of microbial communities. However, differences in soil microbial activity, as measured by basal soil respiration (bSR), were observed. An increase in bSR was found with shrub removal via mechanical clearing, as evidenced by the constants of the single-compartment model and the average residence time (ART) of organic matter. These changes were primarily driven by the indirect effects of vegetation cover alteration. Shrub cover remained low 2 years after the application of both methods, although prescribed burning resulted in more bare soil and lower plant diversity compared to the cleared plot.
Research on the Damage Characteristics of a UAV Flight Control System Irradiated by a Continuous Laser
Authors: Liu Le , Xu Chengyang , Cai Sheng , Wang Jiamin , Huang Dandan , Yang Kun , Zheng Changbin , Guo Jin 
Published: February 2025

To improve laser anti-UAV technology and UAV laser protection capabilities, research on continuous laser damage to a UAV flight control system has been carried out. Combining waveform and function monitoring, the performance of the flight control system under laser irradiation was observed in real time, and the temperature and ablation process were recorded, which were used to analyze its damage characteristics and thresholds. Our experimental results show that the flight control system had two damage modes: temporary failure and permanent damage. Temporary failure had a temperature threshold, which was on average 450.85 K. All temporarily failed flight control system functions could be restored after cooling and a manual restart, but permanently damaged flight control systems could not be manually restarted. This experiment showed that, within K. All temporarily failed flight control system functions could be restored after cooling and a manual restart, but permanently damaged flight control systems could not be manually restarted. This experiment showed that, within 10 s, the power density required for temporary failure and permanent damage to the flight control system was 28.4 W/cm2 and 42.6 W/cm2, respectively. The power density required for permanent damage was 82.0 W/cm2 within 16 s if the flight control system was encapsulated with aluminum alloy. Based on the circuit fault diagnosis of the flight control system samples that has been permanently damaged, the laser’s thermal effects damaged the diodes and linear regulators, ultimately rendering the flight control system unable to be manually restarted.
Industrial Hemp Finola Variety Photosynthetic, Morphometric, Biomechanical, and Yield Responses to K Fertilization Across Different Growth Stages
Authors: Varga Ivana , Markulj Kulundžić Antonela , Krolo Paulina , Iljkić Dario , Tišma Marina , Kraus Ivan 
Published: February 2025

The growing interest in Cannabis sativa as a highly used crop is present worldwide. There are limited data about the effect of potassium (K) fertilizer on industrial hemp yield for dual purposes (seed and stem production). The current study aimed to investigate the influence of adding two different K fertilizers, KCl and K2SO4, at two growth stages (flowering and ripening) on the productivity and chlorophyll a fluorescence (ChlF) of Cannabis sativa, variety Finola. Before sowing, different K treatments were applied: K1—100 kg ha−1 KCl (60% K) and K2—100 kg ha−1 K2SO4 (52% K, S 17%). The OJIP (O stands for “origin” (minimal fluorescence), P for “peak” (maximum fluorescence), and J and I for inflection points between the O and P levels) data were recorded and used for ChlF transients and individual ChlF parameters during vegetation. At harvest, the stem morphology parameters and yield (plant height, stem weight and diameter, and stem and seed yield), tensile strength, and the modulus of elasticity were determined. The results show the sensitivity of minimal (F0) and maximal fluorescence (Fm), electron transport from QA to intersystem electron acceptors (ET0/(TR0 − ET0)), and electron transport flux until PSI acceptors (RE0/RC) to K fertilization. The parameters that described electron transport (ET0/RC, ψE0, and φE0), performance index on absorption basis (PIABS, TR0/DI0, and φP0), dissipation (DI0/RC), and electron transport to photosystem I (φR0 and δR0/(1 − δR0)) had a reaction only at the growth stage, indicating a change in their activity during the aging of the Cannabis sativa plants. The average stem height was 67.5 cm, and the stem diameter was 0.41 cm. The different K sources did not significantly influence the stem height and diameter, nor the dry stem (on average 12.2 t ha−1) and seed yield (on average 1.85 t ha−1). The tensile strength of individual hemp stems was the highest with K2SO4 (53.32 MPa) and the lowest with KCl (49.25 MPa). The stem stiffness by modulus of elasticity was about 5 GPa on average for all the treatments. In general, the photosynthetic parameters in this study varied more between the growth stages than between the different K fertilizer formulations. Moreover, based on the results of this study, it can be recommended to use both fertilizers, KCl and K2SO4, in dual-purpose industrial hemp production since no significant effect was found for the stem morphometric and biomechanical parameters as well as for the agronomic parameters.
Relationships Between the Quantity and Quality of Pollen and the Quantity of Capped Brood in Apis mellifera carnica and Apis mellifera caucasica
Authors: Młynek Krzysztof , Wnorowska Kalina , Pawluk Grzegorz 
Published: February 2025

An important aspect in improving the efficiency of apiary production is knowledge of factors stimulating the condition of colonies. In view of climate change, one important factor is bee behavior associated with meteorological conditions. Studying the effect of selected meteorological parameters on honeybee colony development can help enhance management of the apiary economy. The aim of the study was to evaluate the connections between the quantity and quality of collected pollen and the quantity of capped brood, as an indicator of the rate of development against the background of weather condition indicators. We studied colonies of two relatively old subspecies: Apis mellifera carnica L. (CR) and Apis mellifera caucasica L. (CC) colonies. It was hypothesized that the quantity of pollen gathered is dependent on meteorological conditions, while the amount of capped brood depends on the quality and quantity of pollen. The study was carried out from April to July. Until May, both groups collected similar amounts of pollen. In the second part of the study, CC collected more pollen (averaging 32.3 g more). CR bees, however, exhibited greater development potential, with 6.32 g more capped brood on average (p ≤ 0.05). The greatest differences were noted in the spring period, which indicates faster development in CR colonies. The capped brood weight was positively correlated (0.391, p ≤ 0.05) with the amount of protein in the pollen. Trends in capped brood mass indicate that CR colonies were stronger during spring development. To some extent, this can be explained by the dependency of this behavior on meteorological conditions, specifically wind speed. In comparison to the CC subspecies (−0.600, p ≤ 0.05), CR bees were less sensitive to this factor, as indicated by the stronger correlation between pollen collection and wind strength (−0.642, p ≤ 0.05). The results indicate that some of the features of the subspecies we studied may have some potential in improving colony strength management in the context of changes related to the course of spring weather in eastern Poland.
Solvability of Singular Fractional-Order Differential Equations with a Perturbation Term
Authors: Kong Linghan , Wang Yongqing 
Published: February 2025

In this article, we study singular fractional-order differential equations with a variable coefficient, namely the linear operator of the differential equation containing a linear term with a variable coefficient. The coefficient a(s) permits singularity at s=0, 1, and the nonlinearity f(s,χ) may be singular at s=0, 1 and χ=0. By utilizing the fixed-point index theory, the existence of positive solutions are derived under sharp conditions concerning spectral radius.