bluuebunny/crossref_metadata_2025_split huggingface dataset:
KLF5-induced BBOX1-AS1 contributes to cell malignant phenotypes in non-small cell lung cancer via sponging miR-27a-5p to up-regulate MELK and activate FAK signaling pathway
Authors: Shi Jiang , Yang Chao , An Jinlu , Hao Dexun , Liu Cong , Liu Jumin , Sun Jing , Jiang Junguang 
Published: April 2021

AbstractBackgroundNon-small cell lung cancer (NSCLC) is a major histological subtype of lung cancer with high mortality and morbidity. A substantial amount of evidence demonstrates long non-coding RNAs (lncRNA) as critical regulators in tumorigeneis and malignant progression of human cancers. The oncogenic role of BBOX1 anti-sense RNA 1 (BBOX1-AS1) has been reported in several tumors. As yet, the potential functions and mechanisms of BBOX1-AS1 in NSCLC are obscure.MethodsThe gene and protein expression was detected by qRT-PCR and western blot. Cell function was determined by CCK-8, colony forming, would healing and transwell assays. Bioinformatics tools, ChIP assays, dual luciferase reporters system and RNA pull-down experiments were used to examine the interaction between molecules. Subcutaneous tumor models in nude mice were established to investigate in vivo NSCLC cell behavior.ResultsBBOX1-AS1 was highly expressed in NSCLC tissues and cells. High BBOX1-AS1 expression was associated with worse clinical parameters and poor prognosis. BBOX1-AS1 up-regulation was induced by transcription factor KLF5. BBOX1-AS1 deficiency resulted in an inhibition of cell proliferation, migration, invasion and EMT in vitro. Also, knockdown of BBOX1-AS1 suppressed NSCLC xenograft tumor growth in mice in vivo. Mechanistically, BBOX1-AS1 acted act as a competetive “sponge” of miR-27a-5p to promote maternal embryonic leucine zipper kinase (MELK) expression and activate FAK signaling. miR-27a-5p was confirmed as a tumor suppressor in NSCLC. Moreover, BBOX1-AS1-induced increase of cell proliferation, migration, invasion and EMT was greatly reversed due to the overexpression of miR-27a-5p. In addition, the suppressive effect of NSCLC progression owing to BBOX1-AS1 depletion was abated by the up-regulation of MELK. Consistently, BBOX1-AS1-mediated carcinogenicity was attenuated in NSCLC after treatment with a specific MELK inhibitor OTSSP167.ConclusionsKLF5-induced BBOX1-AS1 exerts tumor-promotive roles in NSCLC via sponging miR-27a-5p to activate MELK/FAK signaling, providing the possibility of employing BBOX1-AS1 as a therapeutic target for NSCLC patients.
Pathogenicity of Pakistani isolates of Steinernema bifurcatum and S. affine (Rhabditida: Steinernematidae) in management of stored grain pests Lasioderma serricorne and Tribolium castaneum (Coleoptera: Ptinidae, Tenebrionidae)
Authors: Khanum Tabassum Ara , Javed Salma 
Published: April 2021

Abstract
Background
Stored grain products suffer a considerable economic loss due to insect infestations. The resistance of pests to insecticide, and residues on crops are produced by the application of insecticide. Nowadays, incremental necessity has been occurred to find out alternatives to chemicals. As biological control agents, entomopathogenic nematodes (EPNs) attract attention increasingly in the research area recently.

Results
The aim of the study was to evaluate the pathogenicity of Pakistani isolates Steinernema bifurcatum and S. affine against the stored grain pests, Tribolium castaneum and Lasioderma serricorne, under laboratory conditions. Suspensions of nematodes were applied at 4 different concentrations 0, 50, 100, and 200 IJs/ml and 3 variable temperatures 15, 30, and 45°C. Pathogenicity rate was recorded after 48 h of application. The larval stage is found more susceptible than the adult. At 200 IJs, S. bifurcatum showed high mortality of L. serricorne larva (92%) and T. castaneum larva (93%), whereas S. affine showed 90 and 95% mortality of L. serricorne and T. castaneum larva, respectively, at 200 IJs. The results revealed that both species of EPN were able to control and reproduce on L. serricorne and T. castaneum. Maximum mortality was recorded at 45 °C from S. bifurcatum whereas S. affine at 15 °C.

Conclusions
Therefore, S. bifurcatum and S. affine could be suggested as a biological control agent for hot and cold climatic zones, respectively.
Safety planning-type interventions for suicide prevention: meta-analysis
Authors: Nuij Chani , van Ballegooijen Wouter , de Beurs Derek , Juniar Dilfa , Erlangsen Annette , Portzky Gwendolyn , O'Connor Rory C. , Smit Johannes H. , Kerkhof Ad , Riper Heleen 
Published: April 2021

BackgroundSafety planning-type interventions (SPTIs) for patients at risk of suicide are often used in clinical practice, but it is unclear whether these interventions are effective.AimsThis article reports on a meta-analysis of studies that have evaluated the effectiveness of SPTIs in reducing suicidal behaviour and ideation.MethodWe searched Medline, EMBASE, PsycINFO, Web of Science and Scopus from their inception to 9 December 2019, for studies that compared an SPTI with a control condition and had suicidal behaviour or ideation as outcomes. Two researchers independently extracted the data. To assess suicidal behaviour, we used a random-effects model of relative risk based on a pooled measure of suicidal behaviour. For suicidal ideation, we calculated effect sizes with Hedges’ g. The study was registered at PROSPERO (registration number CRD42020129185).ResultsOf 1816 unique abstracts screened, 6 studies with 3536 participants were eligible for analysis. The relative risk of suicidal behaviour among patients who received an SPTI compared with control was 0.570 (95% CI 0.408–0.795, P = 0.001; number needed to treat, 16). No significant effect was found for suicidal ideation.ConclusionsTo our knowledge, this is the first study to report a meta-analysis on SPTIs for suicide prevention. Results support the use of SPTIs to help preventing suicidal behaviour and the inclusion of SPTIs in clinical guidelines for suicide prevention. We found no evidence for an effect of SPTIs on suicidal ideation, and other interventions may be needed for this purpose.
Effective dose estimation in cone-beam computed tomography for dental use by Monte-Carlo simulation optimizing calculation numbers using a step-and-shoot method
Authors: Ozaki Yoshihiro , Watanabe Hiroshi , Kurabayashi Tohru 
Published: April 2021

Objective:The objective of this study was to perform effective dose estimation in cone-beam CT for dental use (CBCT) using a Monte-Carlo simulation employing a step-and-shoot method as well as to determine the optimal number of steps.Methods:We simulated 3DX Accuitomo FPD8 as a CBCT model and estimated the effective doses of a large and a small field of view (FOV) examination against the virtual Rando phantom using a particle and heavy ion transport code system. We confirmed the results compared to those from a thermo-luminescence dosemeter (TLD) system in a real phantom and investigated how the reduced angle calculations could be accepted.Results:The effective doses of both FOVs estimated with each one degree were almost the same as those estimated from the TLD measurements. Considering the effective doses and the itemized organ doses, simulation with 5° and 10° is acceptable for the large and small FOV, respectively. We tried to compare an effective dose with a large FOV as well as with multiple small FOVs covering the corresponding area and found that the effective dose from six small FOVs was approximately 1.2 times higher than that of the large FOVs.Conclusion:We successfully performed a Monte-Carlo simulation using a step-and-shoot method and estimated the effective dose in CBCT. Our findings indicate that simulation with 5° or 10° is acceptable based on the FOV size, while a small multiple FOV scan is recommended from a radiation protection viewpoint.
The Granular Nature of Large Institutional Investors
Authors: Ben-David Itzhak , Franzoni Francesco , Moussawi Rabih , Sedunov John 
Published: April 2021

Large institutional investors own an increasing share of the equity markets in the United States. The implications of this development for financial markets are still unclear. The paper presents novel empirical evidence that ownership by large institutions predicts higher volatility and greater noise in stock prices as well as greater fragility in times of crisis. When studying the channel, we find that large institutional investors exhibit traits of granularity (i.e., subunits within a firm display correlated behavior), which reduces diversification of idiosyncratic shocks. Thus, large institutions trade larger volumes and induce greater price impact. This paper was accepted by David Simchi-Levi, finance.
Association of CXCR4 mRNA Expression with Clinicopathological Aspects of Invasive Breast Carcinoma
Authors: Setyawan Novan Adi , Heriyanto Didik Setyo , Yoshuantari Naomi , Irianiwati Irianiwati 
Published: April 2021

<p class="Abstract">ABSTRACT</p><p><strong> </strong></p><p><strong>Background</strong></p><p>Breast cancer is the most common malignancy in women of which majority histological type is Invasive (Ductal) Carcinoma of No Special Type (NST). The prognosis in breast carcinoma is influenced by many factors such as age, tumor size, degree of histology, and lymph node metastasis. Another factor in the development and metastasis of breast cancer is the chemokine receptor CXCR4 and its ligand, CXCL12. Studies state that the expression of CXCR4 in Breast Invasive Carcinoma associated with clinicopathologic aspects remain unclear. This study aims to determine differences in the level of CXCR4 mRNA expression between clinicopathologic aspects in breast carcinoma..</p><p><strong>Method</strong></p><p>A total of 50 samples of formalin-fixed paraffin-embedded (FFPE) tissues diagnosed as invasive breast carcinoma (NST) are used in this study. Samples are divided into groups, namely with and without lymph node metastasis, age &lt;45 years and&gt; 45 years, small and large size, low grade and high grade. CXCR4 mRNA expression is quantitatively examined by qRT-PCR. CXCR4 mRNA expression differences between various clinicopathologic aspects were analyzed by One-Way ANOVA</p><p><strong>Result</strong></p><p>Of the 50 samples, 26 samples (52%) revealed increased expression of CXCR4 mRNA compared to normal tissue. There were no significant differences in mRNA expression of CXCR4 between various prognostic factors (p&gt; 0.05) such as the status of lymph node metastasis, histologic grading, size, and age. However, the expression of CXCR4 mRNA is increased in breast carcinoma when compared to normal breast tissue. Nonetheless the level of CXCR4 expression alone is not associated to clinicopathologic aspects in invasive breast carcinoma.</p><p><strong>Conclusion</strong></p><p>CXCR4 mRNA expression did not differ significantly between the various clinicopathological aspects of invasive breast carcinoma.</p><p> </p><p><strong><em>Keyword</em></strong><strong>: </strong>invasive breast carcinoma, mRNA of CXCR4, Clinicopathologic aspects</p><p> </p>
Dielectric function of vanadium oxide thin films by thermal annealing
Authors: Canillas Adolf , Güell Frank , Arteaga Oriol , Martínez-Alanis Paulina R. , Vergnat Michel , Rinnert Hervé , Garrido Blas 
Published: April 2021

The dielectric function of

V
O

x

and

V

2

O

5

thin films is determined with the use of a spectroscopic Mueller matrix ellipsometer from 1.5 to 5.0 eV. The complex dielectric function of the films is calculated using the measured Mueller matrices filtered with the Cloude decomposition.

V
O

x

shows high absorption in the UV region, a Tauc–Lorentz gap around 2.4 eV, and non-vanishing absorption in the visible.

V

2

O

5

shows a high absorption band centered at 2.87 eV, an indirect optical band gap at 1.95 eV, and a direct optical band gap at 2.33 eV. The ellipsometric characterization is supported by Raman, x-ray photoelectron, and photoluminescence spectroscopy.
“Kankasha” in Kassala: A prospective observational cohort study of the clinical characteristics, epidemiology, genetic origin, and chronic impact of the 2018 epidemic of Chikungunya virus infection in Kassala, Sudan
Authors: Bower Hilary , el Karsany Mubarak , Adam Abd Alhadi Adam Hussein , Idriss Mubarak Ibrahim , Alzain Ma’aaza Abasher , Alfakiyousif Mohamed Elamin Ahmed , Mohamed Rehab , Mahmoud Iman , Albadri Omer , Mahmoud Suha Abdulaziz Alnour , Abdalla Orwa Ibrahim , Eldigail Mawahib , Elagib Nuha , Arnold Ulrike , Gutierrez Bernardo , Pybus Oliver G. , Carter Daniel P. , Pullan Steven T. , Jacob Shevin T. , Abdallah Tajeldin Mohammedein , Gannon Benedict , Fletcher Tom E. 
Published: April 2021

Background
The public health impact of Chikungunya virus (CHIKV) is often underestimated. Usually considered a mild condition of short duration, recent outbreaks have reported greater incidence of severe illness, fatality, and longer-term disability. In 2018/19, Eastern Sudan experienced the largest epidemic of CHIKV in Africa to date, affecting an estimated 487,600 people. Known locally as Kankasha, this study examines clinical characteristics, risk factors, and phylogenetics of the epidemic in Kassala City.

Methodology/Principal findings
A prospective cohort of 102 adults and 40 children presenting with chikungunya-like illness were enrolled at Kassala Teaching Hospital in October 2018. Clinical information, socio-demographic data, and sera samples were analysed to confirm diagnosis, characterise illness, and identify viral strain. CHIKV infection was confirmed by real-time reverse transcription-PCR in 84.5% (120/142) of participants. Nine (7.5%) CHIKV-positive participants had concurrent Dengue virus (DENV) infection; 34/118 participants (28.8%) had a positive Rapid Diagnostic Test for Plasmodium falciparum; six (5.0%) had haemorrhagic symptoms including two children with life-threatening bleeding. One CHIKV-positive participant died with acute renal injury. Age was not associated with severity of illness although CHIKV-infected participants were younger (p = 0.003).
Two to four months post-illness, 63% of adults available for follow-up (30) were still experiencing arthralgia in one or more joints, and 11% remained moderately disabled on Rapid3 assessment. Phylogenetic analysis showed all CHIKV sequences from this study belonged to a single clade within the Indian Ocean Lineage (IOL) of the East/Central/South African (ECSA) genotype. History of contact with an infected person was the only factor associated with infection (p = 0.01), and likely related to being in the same vector environment.

Conclusions/Significance
Vulnerability to CHIKV remains in Kassala and elsewhere in Sudan due to widespread Aedes aegypti presence and mosquito-fostering household water storage methods. This study highlights the importance of increasing awareness of the severity and impact of CHIKV outbreaks, and the need for urgent actions to reduce transmission risk in households.
Factors affecting utilization of mental health services from Primary Health Care (PHC) facilities of western hilly district of Nepal
Authors: Devkota Gaurav , Basnet Puspa , Thapa Bijay , Subedi Madhusudan 
Published: April 2021

Aim
To explore the factors affecting mental health service utilization from Primary Health Care facilities of Arghakhanchi district, a western hilly district of Nepal.

Background
Mental health service utilization has many facilitating and hindering factors present at different socio-ecological levels. Stigma and lack of awareness in the community have been identified as the major barriers for mental health service demand and access worldwide.

Methods
A cross-sectional qualitative study was conducted in Arghakhanchi district of Nepal in July-August 2019 that collected information through face-to-face In-depth and Key Informant Interviews of three categories of participants selected judgmentally. Thirty-two purposively selected participants from the three categories were interviewed using validated interview guidelines. Thematic analysis was performed using RQDA package for EZR software. Validation of translated transcripts, member checking and inter-coder percent agreement were performed to maintain rigor in the study.

Results
Mental health stigma and inadequate awareness were identified as major factors that caused barriers for mental health service utilization at community level. They also influenced different factors at other socio-ecological levels to act as barriers. Awareness in community along with accessibility and availability of comprehensive mental health services were recommended by the participants for increasing service utilization from Primary Health Care facilities.

Conclusion
Individual, family and community awareness could help reduce and/or eliminate mental health stigma. Accessibility of health facilities and availability of comprehensive mental health services in Primary Health Care facilities could help increase service utilization from those facilities.
Maternal obesity and stillbirth at term; placental pathology—A case control study
Authors: Åmark Hanna , Westgren Magnus , Sirotkina Meeli , Hulthén Varli Ingela , Persson Martina , Papadogiannakis Nikos 
Published: April 2021

Objective
The aim was to explore the potential role of the placenta for the risk of stillbirth at term in pregnancies of obese women.

Methods
This was a case-control study comparing placental findings from term stillbirths with placental findings from live born infants. Cases were singleton term stillbirths to normal weight or obese women, identified in the Stockholm stillbirth database, n = 264 and n = 87, respectively. Controls were term singletons born alive to normal weight or obese women, delivered between 2002–2005 and between 2018–2019. Placentas were compared between women with stillborn and live-born infants, using logistic regression analyses.

Results
A long and hyper coiled cord, cord thrombosis and velamentous cord insertion were stronger risk factors for stillbirth in obese women compared to normal weight women. When these variables were adjusted for in the logistic regression analysis, also adjusted for potential confounders, the odds ratio for stillbirth in obese women decreased from 1.89 (CI 1.24–2.89) to 1.63 (CI 1.04–2.56).

Conclusion
Approximately one fourth of the effect of obesity on the risk of stillbirth in term pregnancies is explained by umbilical cord associated pathology.
Epidemiology, outcomes, and utilization of intensive care unit resources for critically ill COVID-19 patients in Libya: A prospective multi-center cohort study
Authors: Elhadi Muhammed , Alsoufi Ahmed , Abusalama Abdurraouf , Alkaseek Akram , Abdeewi Saedah , Yahya Mohammed , Mohammed Alsnosy , Abdelkabir Mohammed , Huwaysh Mohammed , Amkhatirah Emad , Alshorbaji Kamel , Khel Samer , Gamra Marwa , Alhadi Abdulmueti , Abubaker Taha , Anaiba Mohamed , Elmugassabi Mohammed , Binnawara Muhannud , Khaled Ala , Zaid Ahmed , Msherghi Ahmed 
Published: April 2021

BackgroundThe coronavirus disease (COVID-19) pandemic has severely affected African countries, specifically the countries, such as Libya, that are in constant conflict. Clinical and laboratory information, including mortality and associated risk factors in relation to hospital settings and available resources, about critically ill patients with COVID-19 in Africa is not available. This study aimed to determine the mortality and morbidity of COVID-19 patients in intensive care units (ICU) following 60 days after ICU admission, and explore the factors that influence in‐ICU mortality rate.MethodsThis is a multicenter prospective observational study among COVID-19 critical care patients in 11 ICUs in Libya from May 29th to December 30th 2020. Basic demographic data, clinical characteristics, laboratory values, admission Sequential Organ Failure Assessment (SOFA) score, quick SOFA, and clinical management were analyzed.ResultWe included 465 consecutive COVID-19 critically ill patients. The majority (67.1%) of the patients were older than 60 years, with a median (IQR) age of 69 (56.5–75); 240 (51.6%) were male. At 60 days of follow-up, 184 (39.6%) were discharged alive, while 281 (60.4%) died in the intensive care unit. The median (IQR) ICU length of stay was 7 days (4–10) and non-survivors had significantly shorter stay, 6 (3–10) days. The body mass index was 27.9 (24.1–31.6) kg/m2. At admission to the intensive care unit, quick SOFA median (IQR) score was 1 (1–2), whereas total SOFA score was 6 (4–7). In univariate analysis, the following parameters were significantly associated with increased/decreased hazard of mortality: increased age, BMI, white cell count, neutrophils, procalcitonin, cardiac troponin, C-reactive protein, ferritin, fibrinogen, prothrombin, and d-dimer levels were associated with higher risk of mortality. Decreased lymphocytes, and platelet count were associated with higher risk of mortality. Quick SOFA and total SOFA scores increase, emergency intubation, inotrope use, stress myocardiopathy, acute kidney injury, arrythmia, and seizure were associated with higher mortality.ConclusionOur study reported the highest mortality rate (60.4%) among critically ill patients with COVID-19 60 days post-ICU admission. Several factors were found to be predictive of mortality, which may help to identify patients at risk of mortality during the ongoing COVID-19 pandemic.
Estudo laboratorial do comportamento mecânico de agregados reciclados para aplicação em reaterro de valas
Authors: Carvalho João Paulo Barbosa , Motta Rosângela dos Santos 
Published: April 2021

Este trabalho visa contribuir com a avaliação de agregados reciclados para uso em pavimentação, particularmente em reaterro de valas, possibilitando, assim, o reaproveitamento de materiais e buscando a melhoria da qualidade do pavimento reparado. Geralmente as especificações acerca do assunto indicam somente uma caracterização física básica dos materiais neste tipo de aplicação, deixando uma lacuna quanto ao seu comportamento mecânico. Para contribuir neste âmbito, o principal objetivo desta pesquisa é analisar o comportamento mecânico de agregados reciclados de resíduo de construção e demolição (RCD) e de reclaimed asphalt pavement (RAP) em laboratório. O RCD foi avaliado com e sem adição de cimento Portland, enquanto o RAP foi estudado na forma de mistura reciclada a frio com espuma de asfalto. Foi analisado o comportamento mecânico por meio de ensaios de módulo de resiliência e deformação permanente, sob carga repetida, além das evoluções de deformação permanente segundo a teoria do Shakedown. Adicionalmente, os resultados obtidos permitiram a proposição de seções-tipo com estes materiais reciclados em reaterro de valas. Os resultados mostram, de maneira geral, que o RCD estabilizado com cimento e o RAP podem ser aplicados como material de preenchimento de valas em diferentes tipos de tráfego, segundo as condições propostas.
Esophageal Mucosa Innervation in Children With Nonerosive Reflux Disease
Authors: Nikaki Kornilia , Lee Chung , Ustaoglu Ahsen , Alessandrella Annalisa , Staiano Annamaria , Woodland Philip , Sifrim Daniel 
Published: April 2021

INTRODUCTION:
Esophageal mucosa innervation in adults with nonerosive reflux disease (NERD) is more superficial compared with healthy volunteers. We delineated the esophageal mucosal innervation in pediatric NERD and controls.

METHODS:
Distal and proximal pediatric esophageal biopsies were immunohistochemically stained with calcitonin gene-related peptide and transient receptor potential cation channel subfamily V member 1.

RESULTS:
Mucosal innervation was assessed in 18 controls (9M:9F, median age: 9 years) and 11 NERD patients (6M:5F, median age: 5 years). Calcitonin gene-related peptide positive nerve fibers were lying deep in the mucosa in both groups, P > 0.05 and did not coexpress transient receptor potential cation channel subfamily V member 1.

DISCUSSION:
The pediatric esophageal mucosa in NERD displays deep lying nerve fibers, in contrast to adults.