bluuebunny/crossref_metadata_2025_split huggingface dataset:
CircRNA-DAPK1 promotes vascular cell pyroptosis in diabetes by regulating the circ-DAPK1/miR-4454/TXNIP axis.
Authors: Chen Tianchi , Zheng Xiangtao , He Yangyan , Qiu Chenyang , Wang Xiaohui , Xiang Yilang , Zhu Qianqian , Zeng Qinglong , Shang Tao , He Yunjun , Li Donglin , Wu Ziheng , Zhang Hongkun 
Published: February 2021

Abstract
Background

Circular RNAs have been demonstrated to play an important role in the development of vascular diseases. However, little is known about the role of circ-021774, also named circ-DAPK1, in vascular cell pyroptosis.
Methods

Circ-DAPK1 was selected from circular RNA sequencing data of HUVECs treated with high glucose medium and normal medium. RT-qPCR was used to determine the expression of circ-DAPK1 in vivo and in vitro. Dual luciferase reporter assay, fluorescence in situ hybridization (FISH) and RNA immunoprecipitation (RIP) were performed to prove the interaction of circ-DAPK1, miRNA-4454 and thioredoxin-interactingprotein (TXNIP). Adeno-associated virus (AAV) was injected intravenously to establish mouse models. PI staining, western-blot and transmission electron microscopy (TEM) analyses were performed to identify the role of circ-DAPK1 in promoting pyroptosis.
Results

We found that circ-DAPK1 was highly expressed in high glucose medium cultured HUVECs and db/db mice. In vitro and in vivo experiments demonstrated that circ-DAPK1 knockdown decreased the number of PI+ cells, the expression of ASC, NLRP3, GSDMD-N, cleaved caspase-1, IL-18 and IL-1β. In a mechanistic study, the circ-DAPK1/miRNA-4454/TXNIP signaling axis was demonstrated to promote vascular cell pyroptosis in diabetes.
Conclusions

Circ-DAPK1 functions as a promoter of vascular cell pyroptosis in diabetes via the circ-DAPK1/miRNA-4454/TXNIP signaling axis.
Application of Modified Laparoscopic Radical Hysterectomy in The Treatment of Early Cervical Cancer
Authors: Li Xuqing , Pei Xueting , Li Hongyan , Wang Yan , Zhou Youwei , Wei Zhaolian , Yin Zongzhi 
Published: February 2021

Abstract
Background: To investigate the safety and efficacy of modified laparoscopic radical hysterectomy (MLRH) in the treatment of early stage cervical cancer by comparing relevant indices of different surgical procedures in patients with early stage cervical cancer.Methods: Patients with 2014 International Federation of Gynecology and Obstetrics (FIGO) clinical stages IB1 and IIA1 cervical cancer who underwent radical hysterectomy in the Gynecological Department of our hospital from October 2015 to June 2018 were enrolled. Patients were divided into two groups based on the surgical procedure: the open radical hysterectomy (ORH) group (n=336) and MLRH group (n=302). We retrospectively analyzed and compared the clinical characteristics, surgical indices, and survival prognosis between the groups.Results: Compared to the ORH group, the MLRH group exhibited a longer operative time, normal bladder function recovery time, less intraoperative blood loss volume, and more harvested pelvic lymph nodes (P<0.05). No significant differences were observed in postoperative complications, the 2.5-year overall survival (OS) rate, 2.5-year disease-free survival (DFS) rate, and recurrence rate between the groups (P>0.05), but the recurrence pattern was significantly different between the groups (P<0.05). Stratified analysis revealed that OS time was shorter in the ORH group than in the MLRH group in patients with stage IB1 and middle invasion (P<0.05). Pathological type was an independent factor for DFS and OS in early stage cervical cancer.Conclusion: MLRH incorporates a series of measures to prevent tumor spillage. It is a feasible and effective surgical procedure for the treatment of early stage cervical cancer.Trial registration: Present research is a retrospective study. The study had retrospectively registered on Chinese Clinical Trial Registry (http://www.chictr.org.cn/) and the registered number is ChiCTR1900026306.
A Novel Precast Concrete Beam–Column Connection With Replaceable Energy-Dissipation Connector: Experimental Investigation and Theoretical Analysis
Authors: Tong Chao , Wu Jing , Li Chunyu 
Published: February 2021

Abstract
In order to avoid the damage of the connection due to the overstrength of beam strength, and the alternation of the strength hierarchy in the structural system. The additional bending moment produced by the combined action of the pressure generated by the concrete compression zone and the tension generated by the reinforcements needs to be reduced. A novel precast concrete beam–column connection is proposed herein. In the proposed connection, the precast beam is laid on a steel corbel embedded in the precast column. A novel replaceable energy-dissipation connector (REDC) is placed at the bottom of the steel corbel, which ensures that it is in the same horizontal position aligned with the longitudinal reinforcement at the bottom of the connection. In addition, there is a narrow vertical slot adjacent to the column face. The total yield capacity of the top reinforcement is larger than that of the bottom REDC energy-dissipation connector. Theory study focused on the structural features and mechanical mechanism of this novel precast connection. Three low-cycle quasi-static loading tests were carried out on a single full-scaled specimen by replacing three REDCs with different sizes. The cracking pattern of this novel precast connection, and the effects of different parameters of the REDCs on the energy-dissipation capacity and load capacity of the connections were discussed. By performing a finite-element simulation, a method for reducing the additional bending moment and keeping the top reinforcements always in elastic was developed. In addition, the relevant design suggestions were provided. The conclusion shows that the seismic performance of this novel precast concrete beam–column connection is excellent. The positive and negative bending moments of novel precast concrete beam–column connection both reduce the additional bending moment. In view of this, the bending moment of the novel connection is a controllable variable.
Endovascular Coiling versus Microsurgical Clipping for Ruptured Intracranial Aneurysms: A Meta-Analysis and Systematic Review.
Authors: Peng Chao , Diao Yu-hang , Cai Shi-fei , Yang Xinyu 
Published: February 2021

Abstract
Objectives: The purpose of this analysis is to evaluate the current evidence with regard to efficiency and safety between coiling and clipping in patients with ruptured intracranial aneurysms (RIAs).Methods: We performed a meta-analysis that compared clipping with coiling between July 2000 and September 2019. PubMed, EMBASE, and the Cochrane Library were searched for related articles systematically. Results: We identified three randomized controlled trials and thirteen-six observational studies involving 60217 patients with ruptured cerebral aneurysms. The summary results showed that coiling was related a better quality of life (mRS0-2; OR=1.462; CI=1.2375-1.676; P=0.000), a higher risk of mortality (OR=1.116; CI=1.054-1.180; P=0.000), higher rate of rebleeding (RR=1.410; CI=1.092-1.822; P=0.000), lower incidence of vasospasm (OR=0.787; CI=0.649-0.954; P=0.015), lower risk of hydrocephaly (RR=1.143; CI=1.043-1.252; P=0.004), lower risk of cerebral infarction (RR=0.669; CI=0.596-0.751; P=0.000), lower risk of neuro deficits(RR=0.720; CI=0.582-0.892; P=0.003), lower pulmonary complications(RR=0.456; CI=0.232-0.896; P=0.023), and shorter length of stay in hospital and ICU(WMD=-2.290; CI=-3.423--1.157; P=0.000, SMD=-0.346; CI=-0.459--0.234; P=0.000, respectively), a lower rate of complete occlusion(OR=0.495; CI=0.280-0.876; P=0.016). There were no significant difference with regard to the result of GOS (4-5) and the incidence of seizure, intracranial infection.Conclusion: Coiling was significantly associated with a better life quality (mRS0-2), a lower incidence of postoperative complications (vasospasm, hydrocephaly, cerebral infarction, neuro deficits, pulmonary complications), and a higher rate of mortality, rebleeding than clipping. What’s more, coiling was associated with a lower rate of complete occlusion. There was no significant difference about seizure and intracranial infection and the result of GOS (4-5) between the two groups.
Landslide Susceptibility Mapping Using GIS-based Information Value and Frequency Ratio Methods in Gindeberet area, West Shewa Zone, Oromia Region, Ethiopia
Authors: Genene Alembante , Meten Matebie 
Published: February 2021

Abstract
The study area is found in Gindeberet district of West Shewa zone in Oromia Regional State of Ethiopia.This area is highly susceptible to active surface processes due to the presence of rugged morphology with steep scarps, sharp ridges, cliffs, deep gorges and valleys. This study aimed to identify and evaluate the causative factors and to prepare the landslide susceptibility maps (LSMs) of the study area. Two bivariate statistical models i.e. Information value(IV) and the Frequency ratio(FR), were used. First, active, reactivated and passive landslides and scarps were identified using Google Earth image interpretation and extensive field survey for landslide inventory. A total of 580 landslide were randomly selected into two datasets in which (80%)460 landslides were used for modeling and (20%)116 landslidesfor validation. conditioning factors (slope, aspect, curvature, distance from stream, distance from lineaments, lithology, rainfall and land use) were combined with a training landslide dataset in a ArcGIS to generate LSMs which weredivided into verylow, low, moderate, high and veryhigh susceptibility zones. LSMs for IV and FR models were validated using the Area under(ROC) curve showing a success rate of 0.836 and 0.835 respectively and a predictive rate of 0.817 and 0.818 respectively wich showed a good performance of both models. The resulting LSMs can be used for land use planning and management.
In Vitro Propagation and Nuclear DNA Content of Tripleurospermum Insularum (Asteraceae) – A Critically Endangered Insular Endemic Species From Turkey
Authors: Inceer Huseyin , Cuce Mustafa , Imamoglu Kemal Vehbi , Ergin Tugba , Ucler Ali Omer 
Published: February 2021

Abstract
Tripleurospermum insularum Inceer & Hayirlioglu-Ayaz (Asteraceae) is a critically endangered endemic species in Turkey that is face the risk of extinction as a result of the fragmentation of its habitat as well as overgrazing and trampling of its natural population. However, the protocol for micropropagation of this threatened species has not been developed yet. Here, its regeneration ability on MS media supplemented with different plant growth regulators were evaluated using nodal segments. The higher number and length of shoot per explant was achieved with the addition 4.6 µM ZEA and 0.5 µM IAA to the culture medium. Besides, the highest node number of shoot per explant was obtained from MS medium supplemented with 4.6 µM ZEA and 0.5 µM IBA. Flow cytometric analysis also revealed that most of the in vitro developed shoots of T. insularum possessed similar nuclear DNA content as well as ploidy level as initial material and plants from natural population. In vitro rooting of shoots was achieved at 100 % efficiency containing 2.9 µM IAA. Rooted and well-developed plantlets were initially acclimatized under greenhouse conditions and then moved to the botanical garden, where they matured and flowered. Finally, 76% and 74% survivals were achieved during the acclimatization process, respectively. This is the first report of a successfully developed micropropagation protocol of threatened T. insularum for its ex situ conservation.
An Unusual Presentation of Pyelonephritis: Is it COVID-19 Related?
Authors: Hof Liseanne van 't , Pellikaan Lucy , Soonawala Darius , Roshani Hossain 
Published: February 2021

Abstract
IntroductionIn severe cases of COVID-19, late complications such as coagulopathy and organ injury are increasingly described. In milder cases of the disease, the exact time frame and causal path of late onset complications has not yet been determined. Although direct and indirect renal injury by SARS-CoV-2 has been confirmed, hemorrhagic renal infection or coagulative problems in the urinary tract have not yet been described.Case PresentationThis case report describes a 35-year-old female without relevant medical history who, five days after having recovered from an infection with SARS-CoV-2, had an unusual course of acute pyelonephritis of the right kidney and persistent fever under targeted antibiotic treatment. A hemorrhagic ureteral obstruction and severe swollen renal parenchyma preceded the onset of fever and was related to the developing pyelonephritis. Sudden thrombotic vascular occlusion in the right eye appeared during admission. Symmetrical paresthesia in de limbs in combination of severe lower back pain and gastro-intestinal manifestation was documented and not been explained despite of intensive investigation.ConclusionWe present the unusual combination of culture confirmed bacterial hemorrhagic pyelonephritis with a blood clot in the proximal right ureter, complicated by a retinal venous thrombosis, in a patient who had recovered from SARS-CoV-2-infection five days before presentation. The case is suspect of a COVID-19 related etiology.
Predictors of Transient Congenital Primary Hypothyroidism: Data from the German Registry for Congenital Hypothyroidism (AQUAPE "HypoDok")
Authors: Matejek Nicola , Tittel Sascha R. , Haberland Holger , Rohrer Tilman , Busemann Eva-Maria , Jorch Norbert , Schwab Karl-Otfried , Wölfle Joachim , Holl Reinhard W. , Bettendorf Markus 
Published: February 2021

Abstract
Neonatal screening for congenital primary hypothyroidism (CH) may not distinguish between transient (TCH) and permanent dysfunction (PCH), causing potential overtreatment and concerns in affected families. To specify the indication for interruption of therapy we analysed the German registry "HypoDok" for infants with CH, which oversees 1,625 patients from 49 participating centres in Germany and Austria from 1997 until today. 357 Patients with a thyroid gland in loco typico were identified and retrospectively grouped according to cessation (TCH n=24) or continuation (PCH n=333) of L-Thyroxine (L-T4) treatment at 2 years of age. The receiver operating characteristic (ROC) analysis was performed to identify cut-offs predicting TCH by screening TSH concentrations and L-T4 dosages. Gestational ages, birth weights and prevalence of associated malformations were comparable in both groups. The cut-off screening TSH concentration was 73 mU/L. The cut-off daily L-T4 dosage at 1 year was 3.1 µg/kg (90% sensitivity, 63% specificity; 36 µg/d) and at 2 years of age 2.95 µg/kg (91% sensitivity, 59% specificity; 40 µg/d). At 2 years of age, specificity (71%) increased when these both parameters were considered together. Conclusion: The decision to continue or cease L-T4 treatment at 2 years of age in CH patients diagnosed in neonatal screening may be based on their screening TSH concentrations and individual L-T4 dosages at 1 and 2 years of age. Thus, TCH and PCH may be distinguished; overtreatment avoided, and affected families reassured.
Single Crystalline Highly Pure Long Ag2Te Nanowires for Thermoelectric Applications and its Structural Analysis.
Authors: Pillai Krishnakumar 
Published: February 2021

Abstract
Ag2Te is a relatively unexplored promising material, having a variety of potential applications. This paper reports the growth of highly uniform and single crystalline Ag2Te nanowires by a simple vapor transport method without using a catalyst. The morphology, structure and phase purity of the nanowires were investigated by X-ray diffraction, scanning electron microscopy and transmission electron microscopy. The chemical composition of the individual nanowire was examined by X-ray energy-dispersive spectrometry. Our results revealed the growth of monoclinic Ag2Te along (Ī13) planes. At lower temperatures, hexagonal Te nanowires instead of Ag2Te nanowires were grown along (001) planes. This strong dependence on the growth temperature suggests a candidate growth mechanism.
Insights into Molecular Mechanisms Underlying NAFLD by Protein-Protein Interaction Network Analysis of the Differentially Expressed Genes in NASH Patients.
Authors: Najari Aida , Masoud Aminiyanfar , Meysam Mobasheri , Sobati Tabassom 
Published: February 2021

Abstract
The efficient treatment of the disease such as non-alcoholic fatty liver disease as a global concern is impeded by the limited understanding of the disease complexity. We applied a systems-biology framework for a functional analysis of disease development using candidate genes. We identified, the keys dysregulation, pathways, and Hub-genes leading the disease. Moreover, we constructed a molecular disease network for the disease via integrating the pathways, providing an intuitional view to understand the development. In addition, the druggability of genes and their new application in terms of marker discovery or new drug discovery were examined. Our analyses identify a series of highly overexpressed genes, also featuring high centrality, yet being less underlined in the context of NAFLD development in the previous research. We also found several relatively low overexpressed genes, having a central place in the PPI network topology, whose potential involvement in non-alcoholic steatohepatitis might be undermined otherwise using network biology. Our results reveal that the biological processes related to disease progression are complex. Indeed, our pathway studies identified Hub genes, which may reinforce poly-pharmacological cross-talks followed by disease progression to irreversible stages holistic approaches via systems biology tools help us identify more reliable targets and disease-mediated genes integrated into translational studies and drug development in this area.
A Method of Pedestrian Flow Monitoring Based on Received Signal Strength
Authors: Yang Zhiyong , Wen Jing , Huang Kaide 
Published: February 2021

Abstract
In large public places such as scenic tourist areas, shopping malls, stations, squares, and so on, there is a wide demand for people counting and pedestrian flow monitoring. Based on the feedback from the pedestrian flow monitoring system, resources can be optimally allocated to maximize social and economic benefits. Moreover, trampling accidents can be avoided because pedestrian guidance is carried out in time. In order to meet these requirements, we propose a method of pedestrian flow monitoring based on the received signal strength (RSS) of wireless sensor networks. This method mainly utilizes the shadow attenuation effect of pedestrians on RF signals of effective links. In this paper, a deployment structure of a radio frequency wireless sensor network is firstly designed to monitor the pedestrians. Secondly, the features are extracted from the wavelet decomposition of RSS signal series with a short time. Lastly, the support vector machine (SVM) algorithm is trained by an experimental data set to distinguish the instantaneous number of pedestrians passing through the monitoring point. The experimental results show that the accuracy is about 92.9% in the context of real-time pedestrian flow monitoring.In large public places such as scenic tourist areas, shopping malls, stations, squares, and so on, there is a wide demand for people counting and pedestrian flow monitoring. Based on the feedback from the pedestrian flow monitoring system, resources can be optimally allocated to maximize social and economic benefits. Moreover, trampling accidents can be avoided because pedestrian guidance is carried out in time. In order to meet these requirements, we propose a method of pedestrian flow monitoring based on the received signal strength (RSS) of wireless sensor networks. This method mainly utilizes the shadow attenuation effect of pedestrians on RF signals of effective links. In this paper, a deployment structure of a radio frequency wireless sensor network is firstly designed to monitor the pedestrians. Secondly, the features are extracted from the wavelet decomposition of RSS signal series with a short time. Lastly, the support vector machine (SVM) algorithm is trained by an experimental data set to distinguish the instantaneous number of pedestrians passing through the monitoring point. The experimental results show that the accuracy is about 92.9% in the context of real-time pedestrian flow monitoring.
Environmental footprints of soybean production in China
Authors: Chen Wei , Zhang Tianzuo , Tian Xu , Zhai Yiji , Geng Yong , Hong Jinglan , Liu Wenjing , Zhang Qian , Shen Xiaoxu , Bai Yueyang 
Published: February 2021

Abstract
The agriculture sector is both a significant consumer of energy and water and a major source of environmental pollution and greenhouse gases. Soybean production (Glycine max) has experienced a fast growth and it is the fourth most widely cultivated crop, leading to serious environmental concerns. This study evaluates the energy, carbon, and water footprints of China’s soybean production so that key environmental impacts can be identified. To provide reliable results for decision-making, uncertainty analysis is conducted based on the Monte Carlo model. Results show that the impact on climate change, fossils depletion, ecosystem quality, human health, and resource was 3.33×103 kg CO2 eq (GSD2 = 1.87), 343.37 kg oil eq (GSD2 = 1.60), 6.18×10− 5 Species·yr (GSD2 = 1.81), 3.26×10− 3 DALY (GSD2 = 1.81), and 89.22 $ (GSD2 = 2.28), respectively. Freshwater ecotoxicity was the dominant contributor (77.69%) to the ecosystem quality category, while climate change (85.22%) was the dominant contributor to the human health category. Key factors analysis results show that diammonium phosphate and diesel, and on-site emissions from soybean production, were the major contributors to the overall environmental burden of soybean production. Several policy recommendations are proposed, focusing on trade structure optimization, technological improvements, and efficient resource use. Such policy recommendations provide valuable insights to those decision-makers so that they can prepare appropriate mitigation policies.