bluuebunny/crossref_metadata_2025_split huggingface dataset:
Pedigree Reconstruction and Spatial Analysis for Genetic Testing and Selection In A Larix Kaempferi (Lamb.) Carrière Plantation
Authors: Lee Kyungmi , Kim In-Sik , Kang Kyu-Suk 
Published: September 2021

Abstract
Background: Larix kaempferi is one of the major timber species in Northeast Asia. Demand for the reforestation of the species is rising in South Korea due to an increase in large timber production and utilization. However, progeny trials for the species have not been explored, making it challenging to foster advanced generations of tree improvement. In the present study, genetic testing and selection for diameter growth were conducted using pedigree reconstruction and phenotypic spatial distribution analysis in a plantation of L. kaempferi. The aim of the present study was to select the superior larch individuals using the pedigree reconstruction and phenotypic spatial distribution to substitute progeny trials. The plantation of seed orchard crops was established in 1990 and one-hundred and eighty-eight trees were selected as the study material. Genetic variation was investigated first to validate its adequacy as breeding material. Genetic testing was carried out using a model considering pedigree information and spatial autoregression of the phenotypes. The major environmental effects on growth were assessed to understand the site characteristics where the breeding materials were located.Results: The expected heterozygosity of the mother trees and offspring were 0.672 and 0.681 presenting the corresponding level of genetic variation between two groups. The pedigree reconstruction using maternity analysis assigned one to six progenies to ninety-two candidate mothers. The accuracy of genetic testing was exceedingly increased with the animal model considering AR1⊗AR1 structure compared to the animal model only. The estimated genetic variance of the former was 9.086 whereas that of the latter was 4.9E-5. The predicted breeding values of the offspring were ranged from -5.937 to 5.655 and the estimated heritability of diameter growth was 0.344. Shifts in the effects of the factors were detected using geographically weighted regression analysis, which provided information on the post-sectioning of the plantation. Conclusions: The genetic testing approach based on pedigree reconstruction and phenotypic spatial distribution analysis was considered a useful analytical scheme that could replace or supplement progeny trials. Subdivision of the plantation as blocks of the progeny testing was accomplished a posteriori based on the changes of the environmental effects.
Mechanisms of Motor Symptom Improvement by Long-Term Tai Chi Training in Parkinson's Disease Patients
Authors: Li Gen , Huang Pei , Cui Shi-Shuang , Tan Yu-Yan , He Ya-Chao , Shen Xin , Jiang Qin-Ying , Huang Ping , He Gui-Ying , Li Bin-Yin , Li Yu-Xin , Xu Jin , Wang Zheng , Chen Sheng-Di 
Published: September 2021

Abstract
Background
Tai Chi has been shown to improve motor symptoms in Parkinson’s disease (PD), but its long-term effects was not clear and the related mechanism was not elucidated. Therefore, we investigated the mechanism of long-term Tai Chi training on improving motor symptoms in PD.
Methods
95 early-stage PD patients were enrolled and randomly divided into Tai Chi (N = 32), brisk walking (N = 31) and no-exercise group (N = 32). All subjects were assessed at baseline, 6 months and 12 months after one-year intervention. Motor symptoms were evaluated by Berg balance scale (BBS), Unified PD rating scale (UPDRS), Timed Up and Go test (TUG) and 3D gait analysis. Functional MRI, plasma cytokine and metabolomics, blood Huntingtin interaction protein 2 (HIP2) mRNA levels were analyzed to investigate the mechanisms of Tai Chi training at macro and molecular level. The longitudinal effects of self-changes were calculated using repeated measures ANOVA. Generalized estimating equations (GEE) was used to assess the association of longitudinal data of rating scales. Switch rates were used into the fMRI analysis. False discovery rate (FDR) correction was used to perform multiple correction.
Results
Tai Chi group had better performance in BBS, UPDRS, TUG and step width. Besides, Tai Chi had more advantages in improving BBS, step width than brisk walking. Improved BBS was correlated with enhanced visual network function and downregulation of IL-1β. Improvements in UPDRS were associated with enhanced default mode network function, decreased L-malic acid and 3-phosphoglyceric acid, increased adenosine and HIP2 mRNA levels. In addition, arginine biosynthesis, urea cycle, TCA cycle and beta oxidation of very long chain fatty acids were also improved by Tai Chi.
Conclusions
Long-term Tai Chi training improved motor function, especially gait and balance, in PD. Enhanced brain network function, reduced inflammation, improved amino acid metabolism, energy metabolism and neurotransmitters metabolism, decreased vulnerability to dopaminergic degeneration might be the mechanisms of Tai Chi effects.
Trial registration:
This study is registered at Chinese Clinical Trial Registry (Registration number: ChiCTR-OPC-16008074; Registration date: March 8, 2016).
Denatonium Benzoate Exposure Promotes IgE-Mediated Mast Cell Degranulation and Allergy By Upregulating FcεRIα Expression
Authors: Xu Huaping , Shi Xiaoyun , Xie Mengting , Xiao Shiyu , Li Xin , Zou Jiexin , Liu Wenfeng , Shao Huming , Zhang Xiaoyan , Chen Hongbing , Shi Linbo 
Published: September 2021

Abstract
Background: Denatonium benzoate (DB), one of the bitterest compounds known to man, is currently added to a wide range of products and is also used for alcohol denaturation. Some reports demonstrated that asthmatic symptoms are associated with DB exposure but the possible links between DB and IgE-mediated allergy susceptibility have not been examined to date. We investigated the effects of DB on IgE-mediated mast cell degranulation in vitro and in the ovalbumin (OVA)-induced mouse model of allergy.Methods: DB treatments were given to RBL-2H3 IgE-sensitized rat mast cell/basophil cells and KU812 human basophilic cells together with OVA-induced allergic BALB/c mice. Allergic mediator release, Ca2+ influx and OVA-specific IgE anaphylactic shock symptoms were measured along with the cell-surface expression of the α-subunit of high-affinity IgE receptor FcεRI on mast cells.Results: DB increases β-hexosaminidase (β-hex) release and Ca2+ mobilization in IgE-mediated activated RBL-2H3 and KU812 cells, and enhanced the cell-surface expression of FcεRIα. DB also promoted the severity of OVA-induced anaphylactic and diarrheic symptoms which was accompanied by mucus thickness in jejunum and the levels of β-hex, histamine and OVA-specific IgE in allergy mice, as well as the levels of FcεRIα mRNA and the FcεRIα proteinin isolated mucosal mast cells. Conclusions: DB treatments can promote the IgE-mediated mast cell degranulation in vitro and OVA-induced allergic susceptibility in mice by upregulating mast-cell-surface FcεR1α expression, providing evidence for DB exposure in promoting allergy susceptibility.
Predictive Factors of Therapeutic Response According to Craniofacial Skeletal Biotype in Patients with Sleep Apnoea Syndrome using Mandibular Advancement Devices: A Pilot Study
Authors: Navarro Rafael Ecija , Karadede Berşan , Ünal Beyza Karadede , Salvador Domingo Martin 
Published: September 2021

Abstract
Background: In the scientific literature, there is no consistency of results regarding the effectiveness of mandibular advancement devices (MADs) for sleep apnoea treatment. We have considered facial growth as an important predictor of treatment. In this study we analysed that how facial biotype can influence the therapeutic effect of MADs according to polysomnographic records in SAHS patients.Methods: The study enrolled 46 patients with mild to moderate SAHS diagnosed by a polysomnographic test. Using cephalometry, we classified all the patients according their facial biotype (mesofacial, brachyfacial or dolichofacial). Shapiro-Wilk test was used to choose the parametric or non-parametric tests. The quantitative variables were described with the arithmetic mean with its standard deviation or the median with its interquartile range. The hypothesis tests used were Pearson’s chi-squared test, paired sample Student's t test, the Wilcoxon test, one-way ANOVA, Kruskal-Wallis test and Mann-Whitney U test. A p < 0.05 was considered statistically significant.Results: The respiratory disturbance index (RDI) results were: brachyfacial patients had a reduction to 15 events/hour (p < 0.001), the mesofacial patients had a reduction to 14 events/hour (p < 0.001) and the dolichofacial patients did not show a significant reduction in the RDI. The oxygen desaturation index (ODI) results were as follows: brachyfacial patients had a reduction in ODI episodes to 45 episodes/hour (p = 0.001), mesofacial patients had a reduction to 18 episodes/hour (p = 0.001). The dolichofacial patients did not show a reduction in this index. As far as the number of arousals, in the brachyfacial group, the number of awakenings with MAD therapy was reduced to 23 events/hour (p = 0.003), while in the mesofacial group, it was reduced to 37 episodes/hour (p = 0.012). The same behaviour was observed in the dolichofacial group, did not have a reduction in the number of awakenings during sleep after MAD therapy.Conclusions: The facial biotype influences the effectiveness of MAD therapy and is considered a good predictive factor. Dolichofacial patients failed to obtain a significant result in the polysomnographic records using a MAD for sleep apnoea treatment.
Social Relations and Contact With General Practitioner in a Middle-aged Danish Population: A Prospective Register- and Survey-Based Cohort Study
Authors: Mikkelsen Anne Sophie Bech , Lund Rikke , Siersma Volkert , Jørgensen Terese Sara Høj , Christensen Ulla , Kristiansen Maria 
Published: September 2021

Abstract
Background: Findings about the relationship between individuals’ social relations and general practitioner (GP) contact are ambiguous as to whether weak social relations are associated with an increased or decreased consultation pattern. Furthermore, social relations may affect GP contact differently for men compared to women, between socioeconomic groups and according to perceived need. The overall aim of the study is to examine the association between functional aspects of social relations, perceived emotional and instrumental social support, the tendency to consult a GP and the frequency of GP contact. Methods: The study comprised 6911 individuals aged 49–61 at baseline from the Copenhagen Aging and Midlife Biobank (CAMB). We conducted a two-part regression to explore the association between perceived emotional and instrumental social support and GP contact (tendency and frequency), controlling for age, sex, occupational social class, cohabitation status and number of morbidities. Conclusions: Results show no overall effect of the perceived social support aspects of social relations on GP contact. Trial registration: The study has been registered and approved by the Danish Data Protection Agency and the local ethical committee (approval No.H-A-2008-126 and No. 2013-41-1814).
A MiRNA Target Prediction Model based on Distributed Representation Learning and Deep Learning
Authors: Sun Yuzhuo , Xiong Fei , Sun Yongke , Zhao Youjie , Cao Yong 
Published: September 2021

Abstract
Background: MicroRNAs (miRNAs) are a kind of non-coding RNA, which plays an essential role in gene regulation by binding to messenger RNAs(mRNAs). Accurate and rapid identification of miRNA target genes is helpful to reveal the mechanism of transcriptome regulation, which is of great significance for the study of cancer and other diseases. Many bioinformatics methods have been proposed to solve this problem, but the previous research did not further study the encoding of the base sequence. Results: In this study, we developed a novel method combining word embedding and deep learning for human miRNA targets at the site level prediction, which is inspired by the similarity between natural language and biological sequences. First, the wor2vec model was used to mine the distribution representation of miRNAs and mRNAs. Then, the data features are fully extracted automatically from temporal and spatial via the stacked Bidirectional Long short-term memory(BiLSTM) network. We compare the effects of different embedding methods on model accuracy in different deep learning models, and the results prove that using word2vec can improve the accuracy of deep learning models. In addition, we performed visual analysis on the distributed represented sequences and found hidden similarity relationships between bases. Finally, compared with different advanced methods and data sets, the results show that our proposed method has gotten better performance in multiple evaluation aspects. Conclusions: We present a novel method for predicting miRNA target sites consisting of word2vec and the BiLSTM model and demonstrate that this method can realize automatic feature extraction and has higher accuracy. Furthermore, we process miRNA and mRNA as two languages for the first time and explore their biological significance through visual analysis.
Rapidly Evolving Deep Dissecting Hematoma With Sudden Onset, Developed One Month After A Minimal Trauma.
Authors: Hamdan Rémy , Zwetyenga Narcisse , Macheboeuf Yvan , Ray Patrick 
Published: September 2021

Abstract
Background: Deep dissecting hematoma is a rapidly extending blood collection that splits the hypodermis from muscle fascia, constituting a medical surgical emergency. The natural history of this condition includes trauma (even minor physical injury) shortly before onset of the lesion, occurring in a patient with advanced dermatoporosis. Case presentation: We report the admission of a 70-year-old woman to the emergency department of our hospital for the onset of a deep dissecting hematoma one month after a negligible trauma in the right leg, complicating secondary iatrogenic dermatoporosis. Bedside ultrasound examination was used to eliminate differential or additional diagnoses and to assess the main features of the hematoma (dimensions, existence of blood supply). Surgical debridement and hematoma drainage were performed due to rapid horizontal extension of the hematoma and unresolved pain, with the operative report confirming the diagnosis. Conclusion: This observation emphasises that in patients with severe dermatoporosis, several weeks can elapse between a minor impact and the sudden development of a limb-threatening deep dissecting hematoma.
Clinical Features, Echocardiographic Findings, Management and Outcome in a Young Male Patient with Atrial Myxoma: A Rare Primary Cardiac Tumor
Authors: Mohamud Mohamed Farah Yusuf , Ahmed Said Abdirahman , Mohamud Mohamed Abdullahi 
Published: September 2021

Abstract
Primary cardiac tumors in pediatric are rare and mostly benign. Atrial Myxoma is the most common primary tumor of the heart, which is usually involved in the left atrium. Surgical treatment is recommended when the patients develop symptoms or hemodynamically is impaired. Cardiac Myxoma can cause left atrial obstruction and systemic embolization. So early diagnosis with surgical intervention is the definitive management of the tumor and should not be delayed. Here we present a case of a 23-year old male who admitted to the emergency department with syncope and dyspnea for 1day ago. The tumor was removed surgically; pathologic examination confirmed the diagnosis of Myxoma.
Digging for the Discovery of SARS-CoV-2 nsp12 Inhibitors: A Pharmacophore-Based and Molecular Dynamics Simulation Study
Authors: Askari Fatemeh Sana , Ebrahimi Mohsen , Parhiz Jabbar , Hassanpour Mina , Mohebbi Alireza , Mirshafiey Abbas 
Published: September 2021

Abstract

Background: The severe acute respiratory syndrome Coronavirus 2 (SARS-CoV-2) is a grave global threat causing Coronavirus Disease 2019 (COVID-19). The therapeutics are urgently needed to cure patients severely infected with COVID-19. The aim of the study was to investigate for potential candidates of nsp12 inhibitors by searching for druggable cavity pockets within the viral protein and drug discovery.Methods: The crystallographic structure of SARS-CoV-2 nsp12 was searched for strong druggable cavity pockets and pharmacophore features by the CavityPlus server. The features were selected for high-throughput screening (HTS) of a chemical library of ZINC natural products and hit identification database by ZINCPharmer. Autodock Vina was furthered utilized for estimation of hits' affinities to nsp12. A lead compound with the highest affinity to nsp12 was simulated dynamically by GROMACS for 10 nanoseconds (ns) to measure the hit stability in complex with nsp12 and conformational changes.Results: 1 of 6 cavities with the highest score was selected for extraction of pharmacophore features and hit-identification. 9 pharmacophores were screened, and a total of unique 1274 hits were identified. One compound, ZINV03977803, with an -11.0 Kcal.mol-1 affinity was selected as the lead compound for molecular dynamic simulation (MDS). The results showed stable interaction between ZINV03977803 and nsp12 during 10 ns of simulation. The room-mean-square of deviation (RMSD) measure showed dramatically high conformational changes in the complex of ZINV03977803 and nsp12 compare two the viral proteins alone.Conclusions: The lead compound ZINV03977803 showed stable interaction with higher potential and hydrogen bonding with the catalytic subunit of SARS-CoV-2, nsp12. It could also inhibit the SARS-CoV-2 life cycle by direct interaction with nsp12 and inhibits RdRp complex formation.
Parallel Mediation Model Linking Type D Personality with Adverse Health Outcomes in People with Type 2 Diabetes
Authors: Lee Eun-Hyun , Lee Young Whee , Chae Duckhee , Kang Eun Hee , Kang Hyun-Jung 
Published: September 2021

Abstract
Background: This study aims to investigate a parallel mediation model to directly link Type D personality with adverse glycated hemoglobin (HbA1c) health outcomes and health-related quality of life (HRQOL), and indirectly through diabetes distress and social isolation in people with type 2 diabetes.Methods: Secondary data analysis of a cross-sectional survey was used. Data on 524 people with type 2 diabetes from June 2020 to February 2021 were recruited from outpatient clinics. The analyzed components of the data set were Type D personality, diabetes distress, social isolation, HbA1c, and health-related quality of life (HRQOL). The hypothesized parallel mediation model was tested using the PROCESS macro for SPSS. A 10,000-bootstrap bias-corrected procedure was used to estimate 95% confidence intervals for indirect effects.Results: Type D personality was present in 31.3% of the participants. The direct effect of Type D personality on HbA1c was insignificant, but was significant on HRQOL. The indirect effects of Type D personality on HbA1c and HRQOL through diabetes distress and social isolation were both significant. The indirect effect on HRQOL through diabetes distress was greater than that through social isolation.Conclusion: People with a Type D personality more often experienced negative emotions in response to the burden of living with and managing diabetes and might have fewer interpersonal contacts, which in turn impair glycemic control and HRQOL. This study identified the mechanisms underlying the relationship between Type D personality and glycemic control and HRQOL in people with type 2 diabetes. Both diabetes distress and social isolation mediated these relationships. Health professionals must monitor people with type 2 diabetes to determine the presence of Type D personality. It is particularly recommended for those with Type D personality to be provided with interventions to reduce diabetes distress and to enhance social isolation in order to improve their glycemic control and HRQOL.
Surface Coal Mining Impacts On Land Use Change And Ecological Service Value: A Case Study In The Shengli Coalfield, Inner Mongolia
Authors: Zhang Lijia , Zhou Xu , Zhou Yan , Zhou Ji , Guo Jiwang , Zhai Zihan , Chen Yan , Su Xiangyan , Ying Lingxiao , Wang Liwei , Qiao Ying 
Published: September 2021

Abstract
Coal plays a crucial role in global economic development, which is still the most common and widely distributed fossil fuel in the world. As the world's largest developing country, China's mining and utilization of coal resources make great contributions to the rapid growth of China's economy. Inner Mongolia lies in the arid and semi-arid areas of China, its ecological environment is very fragile. The exploitation of opencast mining seriously hinders the sustainable use of regional land and the promotion of residents' well-being. This paper uses 2000, 2005, 2010, 2015 and 2020 remote sensing imageries, Based on ENVI, using a random forest algorithm to divide the land utilization type into construction land, vegetation, cultivated land, bare land and water area，and analyses the evolution characteristics of land use and ecosystem service value during the past 20 years. The results showed that: (1) Between 2000 and 2020, the construction land, vegetation and water of mining area were reduced, the bare land and cultivated land showed an increasing trend. The largest ratio changed is bare land, the smallest changed rate is water area. (2) The total ecosystem service value of the mining area reduced from10.939 billion yuan to 9.527 billion yuan. Among them, vegetation ecosystem service value is the highest, followed by cultivated land and water, and bare land ecosystem service value is the lowest. (3) From the spatial scale, the total ecosystem service value of the Shengli mining area decreases year by year, which shows that the land-use change in the mining area does have an impact on the ecosystem service value.