bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Cuticle Collagen Encoded by the lon-3 Gene May Be a Target of TGF-β Signaling in Determining Caenorhabditis elegans Body Shape
Authors: Suzuki Yo , Morris Gail A , Han Min , Wood William B 
Published: April 2021

Abstract
The signaling pathway initiated by the TGF-β family member DBL-1 in Caenorhabditis elegans controls body shape in a dose-dependent manner. Loss-of-function (lf) mutations in the dbl-1 gene cause a short, small body (Sma phenotype), whereas overexpression of dbl-1 causes a long body (Lon phenotype). To understand the cellular mechanisms underlying these phenotypes, we have isolated suppressors of the Sma phenotype resulting from a dbl-1(lf) mutation. Two of these suppressors are mutations in the lon-3 gene, of which four additional alleles are known. We show that lon-3 encodes a collagen that is a component of the C. elegans cuticle. Genetic and reporter-gene expression analyses suggest that lon-3 is involved in determination of body shape and is post-transcriptionally regulated by the dbl-1 pathway. These results support the possibility that TGF-β signaling controls C. elegans body shape by regulating cuticle composition.
A Genome Scan for Quantitative Trait Loci in a Wild Population of Red Deer (Cervus elaphus)
Authors: Slate J , Visscher P M , MacGregor S , Stevens D , Tate M L , Pemberton J M 
Published: April 2021

AbstractRecent empirical evidence indicates that although fitness and fitness components tend to have low heritability in natural populations, they may nonetheless have relatively large components of additive genetic variance. The molecular basis of additive genetic variation has been investigated in model organisms but never in the wild. In this article we describe an attempt to map quantitative trait loci (QTL) for birth weight (a trait positively associated with overall fitness) in an unmanipulated, wild population of red deer (Cervus elaphus). Two approaches were used: interval mapping by linear regression within half-sib families and a variance components analysis of a six-generation pedigree of &gt;350 animals. Evidence for segregating QTL was found on three linkage groups, one of which was significant at the genome-wide suggestive linkage threshold. To our knowledge this is the first time that a QTL for any trait has been mapped in a wild mammal population. It is hoped that this study will stimulate further investigations of the genetic architecture of fitness traits in the wild.
Genetic Analysis, Expression and Molecular Characterization of BoGSL-ELONG, a Major Gene Involved in the Aliphatic Glucosinolate Pathway of Brassica Species
Authors: Li Genyi , Quiros Carlos F 
Published: April 2021

Abstract
We cloned a major aliphatic glucosinolate (GSL) gene, BoGSL-ELONG in Brassica oleracea, using the Arabidopsis sequence database. We based our work on an Arabidopsis candidate gene forming part of a gene family coding for isopropyl malate synthetase-like enzymes (IPMS). This gene is presumably responsible for synthesis of GSL possessing side chains consisting of four carbons (4C). The similarity of the Brassica homolog IPMS-Bo from broccoli to its Arabidopsis counterpart IPMS-At was on the order of 78%, both sharing the same number of exons. A nonfunctional allele of the BoGSL-ELONG gene from white cauliflower, based on the absence of 4C GSL in this crop, displayed a 30-bp deletion, which allowed us to develop a codominant marker for 4C-GSL. Gene expression analysis based on RT-PCR revealed a splicing site mutation in the white cauliflower allele. This resulted in a longer transcript containing intron 3, which failed to excise. Perfect cosegregation was observed for broccoli and cauliflower alleles at the IPMS-Bo gene and 4C-GSL content, strongly indicating that this gene indeed corresponds to BoGSL-ELONG. Cloning of two other major genes, BoGSL-ALK and BoGSL-PRO, is underway. The availability of these genes and BoGSL-ELONG is essential for the manipulation of the aliphatic GSL profile of B. oleracea.
The SONBNUP98 Nucleoporin Interacts With the NIMA Kinase in Aspergillus nidulans
Authors: De Souza Colin P C , Horn Kevin P , Masker Kathryn , Osmani Stephen A 
Published: April 2021

Abstract
The Aspergillus nidulans NIMA kinase is essential for mitotic entry. At restrictive temperature, temperature-sensitive nimA alleles arrest in G2, before accumulation of NIMA in the nucleus. We performed a screen for extragenic suppressors of the nimA1 allele and isolated two cold-sensitive son (suppressor of nimA1) mutants. The sonA1 mutant encoded a nucleoporin that is a homolog of yeast Gle2/Rae1. We have now cloned SONB, a second nucleoporin genetically interacting with NIMA. sonB is essential and encodes a homolog of the human NUP98/NUP96 precursor. Similar to NUP98/NUP96, SONBNUP98/NUP96 is autoproteolytically cleaved to generate SONBNUP98 and SONBNUP96. SONBNUP98 localizes to the nuclear pore complex and contains a GLEBS domain (Gle2 binding sequence) that binds SONAGLE2. A point mutation within the GLEBS domain of SONB1NUP98 suppresses the temperature sensitivity of the nimA1 allele and compromises the physical interaction between SONAGLE2 and SONB1NUP98. The sonB1 mutation also causes sensitivity to hydroxyurea. We isolated the histone H2A-H2B gene pair as a copy-number suppressor of sonB1 cold sensitivity and hydroxyurea sensitivity. The data suggest that the nucleoporins SONAGLE2 and SONBNUP98 and the NIMA kinase interact and regulate nuclear accumulation of mitotic regulators to help promote mitosis.
Effect of the Suppressor of Underreplication (SuUR) Gene on Position-Effect Variegation Silencing in Drosophila melanogaster
Authors: Belyaeva E S , Boldyreva L V , Volkova E I , Nanayev R A , Alekseyenko A A , Zhimulev I F 
Published: April 2021

Abstract
It has been previously shown that the SuUR gene encodes a protein located in intercalary and pericentromeric heterochromatin in Drosophila melanogaster polytene chromosomes. The SuUR mutation suppresses the formation of ectopic contacts and DNA underreplication in polytene chromosomes; SuUR+ in extra doses enhances the expression of these characters. This study demonstrates that heterochromatin-dependent PEV silencing is also influenced by SuUR. The SuUR protein localizes to chromosome regions compacted as a result of PEV; the SuUR mutation suppresses DNA underreplication arising in regions of polytene chromosomes undergoing PEV. The SuUR mutation also suppresses variegation of both adult morphological characters and chromatin compaction observed in rearranged chromosomes. In contrast, SuUR+ in extra doses and its overexpression enhance variegation. Thus, SuUR affects PEV silencing in a dose-dependent manner. However, its effect is expressed weaker than that of the strong modifier Su(var)2-5.
Population Subdivision and Molecular Sequence Variation: Theory and Analysis ofDrosophila ananassaeData
Authors: Vogl Claus , Das Aparup , Beaumont Mark , Mohanty Sujata , Stephan Wolfgang 
Published: April 2021

AbstractPopulation subdivision complicates analysis of molecular variation. Even if neutrality is assumed, three evolutionary forces need to be considered: migration, mutation, and drift. Simplification can be achieved by assuming that the process of migration among and drift within subpopulations is occurring fast compared to mutation and drift in the entire population. This allows a two-step approach in the analysis: (i) analysis of population subdivision and (ii) analysis of molecular variation in the migrant pool. We model population subdivision using an infinite island model, where we allow the migration/drift parameter 0398; to vary among populations. Thus, central and peripheral populations can be differentiated. For inference of 0398;, we use a coalescence approach, implemented via a Markov chain Monte Carlo (MCMC) integration method that allows estimation of allele frequencies in the migrant pool. The second step of this approach (analysis of molecular variation in the migrant pool) uses the estimated allele frequencies in the migrant pool for the study of molecular variation. We apply this method to a Drosophila ananassae sequence data set. We find little indication of isolation by distance, but large differences in the migration parameter among populations. The population as a whole seems to be expanding. A population from Bogor (Java, Indonesia) shows the highest variation and seems closest to the species center.
Quantitative Trait Locus Analysis of Leaf Dissection in Tomato Using Lycopersicon pennellii Segmental Introgression Lines
Authors: Holtan Hans E E , Hake Sarah 
Published: April 2021

Abstract
Leaves are one of the most conspicuous and important organs of all seed plants. A fundamental source of morphological diversity in leaves is the degree to which the leaf is dissected by lobes and leaflets. We used publicly available segmental introgression lines to describe the quantitative trait loci (QTL) controlling the difference in leaf dissection seen between two tomato species, Lycopersicon esculentum and L. pennellii. We define eight morphological characteristics that comprise the mature tomato leaf and describe loci that affect each of these characters. We found 30 QTL that contribute one or more of these characters. Of these 30 QTL, 22 primarily affect leaf dissection and 8 primarily affect leaf size. On the basis of which characters are affected, four classes of loci emerge that affect leaf dissection. The majority of the QTL produce phenotypes intermediate to the two parent lines, while 5 QTL result in transgression with drastically increased dissection relative to both parent lines.
Evolution in Saccharomyces cerevisiae: Identification of Mutations Increasing Fitness in Laboratory Populations
Authors: Blanc Victoria M , Adams Julian 
Published: April 2021

Abstract
Since the publication of the complete sequence of the genome of Saccharomyces cerevisiae, a number of comprehensive investigations have been initiated to gain insight into cellular function. The focus of these studies has been to identify genes essential for survival in specific environments or those that when mutated cause gross phenotypic defects in growth. Here we describe Ty1-based mutational approaches designed to identify genes, which when mutated generate evolutionarily significant phenotypes causing small but positive increments on fitness. As expected, Ty1 mutations with a positive fitness effect were in the minority. However, mutations in two loci, one inactivating FAR3 and one upstream of CYR1, identified in evolving populations, were shown to have small but significantly positive fitness effects.
A giant left internal iliac artery aneurysm in a patient with Loeys–Dietz syndrome
Authors: Skopin Ivan I , Arakelyan Valerii S , Tchurikov Nickolai A , Belyaev Andrei M 
Published: April 2021

Abstract
A 16-year-old female presented with left iliac fossa pain. In January 2021, she was admitted to her local hospital with severe lower abdominal pain and the pelvic ultrasound demonstrated a 13-cm left internal iliac artery dissecting aneurysm with its partial thrombosis. On examination, she had a high-arched palate, multiple skin stretch marks, flat feet and a soft systolic ejection murmur at the left 5th mid-clavicular line. She had a mildly tender abdomen in the left iliac fossa. Computed tomography angiography demonstrated a 12.2 cm × 10.4 cm × 12.5 cm left internal iliac artery aneurysm. During surgery, the aneurysm was incised and the proximal and distal orifices of the internal iliac artery were ligated. Genetic testing yielded 2 mutations in the SMAD3 gene characteristic for Loeys–Dietz syndrome.
Can Consumers Learn Price Dispersion? Evidence for Dispersion Spillover across Categories
Authors: André Quentin , Reinholtz Nicholas , De Langhe Bart 
Published: April 2021

AbstractPrice knowledge is a key antecedent of many consumer judgments and decisions. This article examines consumers’ ability to form accurate beliefs about the minimum, the maximum, and the overall variability of prices for multiple product categories. Eight experiments provide evidence for a novel phenomenon we call dispersion spillover: Consumers tend to overestimate price dispersion in a category after encountering another category in which prices are more dispersed (vs. equally or less dispersed). Our experiments show that this dispersion spillover is consequential: It influences the likelihood that consumers will search for (and find) better prices and offers, and how much consumers bid in auctions. Finally, we disentangle two cognitive processes that might underlie dispersion spillover. Our results suggest that judgments of dispersion are not only based on specific prices stored in memory and that dispersion spillover does not simply reflect the inappropriate activation of prices from other categories. Instead, it appears that consumers also form “intuitive statistics” of dispersion: Summary representations that encode the dispersion of prices in the environment but that are insufficiently category specific.
Refining Pheromone Lures for the Invasive Halyomorpha halys (Hemiptera: Pentatomidae) Through Collaborative Trials in the United States and Europe
Authors: Leskey Tracy C , Andrews Heather , Bády Angelika , Benvenuto Luca , Bernardinelli Iris , Blaauw Brett , Bortolotti Pier Paolo , Bosco Lara , Di Bella Emanuele , Hamilton George , Kuhar Thomas , Ludwick Dalton , Maistrello Lara , Malossini Giorgio , Nannini Roberta , Nixon Laura J , Pasqualini Edison , Preti Michele , Short Brent D , Spears Lori , Tavella Luciana , Vétek Gábor , Wiman Nik 
Published: April 2021

Abstract
Brown marmorated stink bug, Halyomorpha halys, is native to Asia and has invaded North America and Europe inflicting serious agricultural damage to specialty and row crops. Tools to monitor the spread of H. halys include traps baited with the two-component aggregation pheromone (PHER), (3S,6S,7R,10S)-10,11-epoxy-1-bisabolen-3-ol and (3R,6S,7R,10S)-10,11-epoxy-1-bisabolen-3-ol, and pheromone synergist, methyl (2E,4E,6Z)-decatrienoate (MDT). Here, an international team of researchers conducted trials aimed at evaluating prototype commercial lures for H. halys to establish relative attractiveness of: 1) low and high loading rates of PHER and MDT for monitoring tools and attract and kill tactics; 2) polyethylene lure delivery substrates; and 3) the inclusion of ethyl (2E,4E,6Z)-decatrieonate (EDT), a compound that enhances captures when combined with PHER in lures. In general, PHER loading rate had a greater impact on overall trap captures compared with loading of MDT, but reductions in PHER loading and accompanying lower trap captures could be offset by increasing loading of MDT. As MDT is less expensive to produce, these findings enable reduced production costs. Traps baited with lures containing PHER and EDT resulted in numerically increased captures when EDT was loaded at a high rate, but captures were not significantly greater than those traps baited with lures containing standard PHER and MDT. Experimental polyethylene vial dispensers did not outperform standard lure dispensers; trap captures were significantly lower in most cases. Ultimately, these results will enable refinement of commercially available lures for H. halys to balance attraction and sensitivity with production cost.
Database ofSabethesandHaemagogus(Diptera: Culicidae) in Argentina: Sylvatic Vectors of the Yellow Fever Virus
Authors: Cano María Eugenia , Marti Gerardo Aníbal , Balsalobre Agustín , Muttis Evangelina , Bruno Emiliano Aldo , Rossi Gustavo , Micieli María Victoria 
Published: April 2021

AbstractYellow fever is an endemic disease in America caused by an arbovirus that circulates in the sylvatic cycle between nonhuman primates and mosquitoes of the genera Sabethes Robineau-Desvoidy and Haemagogus Williston. The main goal of this work is to report the distribution patterns of these genera in Argentina through an updated database built from published records as well as from own sample collections. These genera are represented in Argentina by a total of 18 species distributed in 14 provinces and 10 ecoregions. The ecoregions with greatest biodiversity were Paranense Forest, Yungas, Campos and Malezales. This database will also allow generating distribution maps for these mosquito genera, and their respective species in Argentina, to establish areas with high probability of viral circulation that are an essential input for vector surveillance, as a tool for public health decision-makers.
Changing Perceptions and Actions in Response to Forest Disturbance by Mountain Pine Beetles in North Central Colorado
Authors: Qin Hua , Brenkert-Smith Hannah , Vickery Jamie , Sanders Christine , Flint Courtney G 
Published: April 2021

AbstractForest disturbances caused by insects, pathogens, and fire continue to increasingly occur within forests across the United States and around the world. Given the dynamic nature of these forest disturbances and the role played by local residents in risk management, it is valuable to explore how the human experience, attitudes, and behaviors associated with these ecological processes may evolve over time. In this paper, we assess temporal changes in local residents’ perceptions and actions in response to the mountain pine beetle outbreak that affected large swaths of forests in north central Colorado. Through analyses of secondary and household survey data from 2007 and 2018, we note significant changes in these aspects and identify factors consistently associated with individual and community activeness. The study contributes to knowledge of dynamic socioecological considerations of forest disturbances and improves understanding of how social sciences can help to identify opportunities and barriers to effective forest ecosystem management.
H-2B Guest Workers and the Critical Role They Play in US Forests
Authors: Blinn Charles R , O’Hara Timothy J , Russell Matthew B 
Published: April 2021

AbstractA survey of businesses that provide a variety of forest management services indicated the importance of H-2B guest workers in performing important tasks which facilitate management and sustainability of forest resources in the United States. Those workers accomplish a wide variety of tasks with tree planting being most common. Although the positions filled by those workers are first made available to US workers at government-mandated wage rates, few apply. Businesses reported that it is becoming increasingly difficult for them to secure all of their guest visas to fulfill their contacts. Although former President Donald J. Trump’s proclamation on June 22, 2020, suspending entry of workers with H-2B visas, would have resulted in approximately one million acres not being reforested by respondents in 2020, a National Interest Exemption process provided a reprieve through the end of 2020. A long-term solution is needed to ensure that there are enough H-2B workers available to continue providing these important services.
A fully labelled proof system for intuitionistic modal logics
Authors: Marin Sonia , Morales Marianela , Straßburger Lutz 
Published: April 2021

AbstractLabelled proof theory has been famously successful for modal logics by mimicking their relational semantics within deductive systems. Simpson in particular designed a framework to study a variety of intuitionistic modal logics integrating a binary relation symbol in the syntax. In this paper, we present a labelled sequent system for intuitionistic modal logics such that there is not only one but two relation symbols appearing in sequents: one for the accessibility relation associated with the Kripke semantics for normal modal logics and one for the pre-order relation associated with the Kripke semantics for intuitionistic logic. This puts our system in close correspondence with the standard birelational Kripke semantics for intuitionistic modal logics. As a consequence, it can be extended with arbitrary intuitionistic Scott–Lemmon axioms. We show soundness and completeness, together with an internal cut elimination proof, encompassing a wider array of intuitionistic modal logics than any existing labelled system.
Ultrasound in Traumatic Spinal Cord Injury: A Wide-Open Field
Authors: Hwang Brian Y , Mampre David , Ahmed A Karim , Suk Ian , Anderson William S , Manbachi Amir , Theodore Nicholas 
Published: April 2021

Abstract
Traumatic spinal cord injury (SCI) is a common and devastating condition. In the absence of effective validated therapies, there is an urgent need for novel methods to achieve injury stabilization, regeneration, and functional restoration in SCI patients. Ultrasound is a versatile platform technology that can provide a foundation for viable diagnostic and therapeutic interventions in SCI. In particular, real-time perfusion and inflammatory biomarker monitoring, focal pharmaceutical delivery, and neuromodulation are capabilities that can be harnessed to advance our knowledge of SCI pathophysiology and to develop novel management and treatment options. Our review suggests that studies that evaluate the benefits and risks of ultrasound in SCI are severely lacking and our understanding of the technology's potential impact remains poorly understood. Although the complex anatomy and physiology of the spine and the spinal cord remain significant challenges, continued technological advances will help the field overcome the current barriers and bring ultrasound to the forefront of SCI research and development.
ABCB1 inhibition provides a novel therapeutic target to block TWIST1-induced migration in medulloblastoma
Authors: Nasir Aishah , Cardall Alice , Othman Ramadhan T , Nicolaou Niovi , Lourdusamy Anbarasu , Linke Franziska , Onion David , Ryzhova Marina , Cameron Hanna , Valente Cara , Ritchie Alison , Korshunov Andrey , Pfister Stefan M , Grabowska Anna M , Kerr Ian D , Coyle Beth 
Published: April 2021

Abstract

Background
Therapeutic intervention in metastatic medulloblastoma is dependent on elucidating the underlying metastatic mechanism. We investigated whether an epithelial–mesenchymal transition (EMT)-like pathway could drive medulloblastoma metastasis.

Methods
A 3D Basement Membrane Extract (3D-BME) model was used to investigate medulloblastoma cell migration. Cell line growth was quantified with AlamarBlue metabolic assays and the morphology assessed by time-lapse imaging. Gene expression was analyzed by qRT-PCR and protein expression by immunohistochemistry of patient tissue microarrays and mouse orthotopic xenografts. Chromatin immunoprecipitation was used to determine whether the EMT transcription factor TWIST1 bound to the promoter of the multidrug pump ABCB1. TWIST1 was overexpressed in MED6 cells by lentiviral transduction (MED6-TWIST1). Inhibition of ABCB1 was mediated by vardenafil, and TWIST1 expression was reduced by either Harmine or shRNA.

Results
Metastatic cells migrated to form large metabolically active aggregates, whereas non-tumorigenic/non-metastatic cells formed small aggregates with decreasing metabolic activity. TWIST1 expression was upregulated in the 3D-BME model. TWIST1 and ABCB1 were significantly associated with metastasis in patients (P = .041 and P = .04, respectively). High nuclear TWIST1 expression was observed in the invasive edge of the MED1 orthotopic model, and TWIST1 knockdown in cell lines was associated with reduced cell migration (P &lt; .05). TWIST1 bound to the ABCB1 promoter (P = .03) and induced cell aggregation in metastatic and TWIST1-overexpressing, non-metastatic (MED6-TWIST1) cells, which was significantly attenuated by vardenafil (P &lt; .05).

Conclusions
In this study, we identified a TWIST1–ABCB1 signaling axis during medulloblastoma migration, which can be therapeutically targeted with the clinically approved ABCB1 inhibitor, vardenafil.