bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Step in the Right Direction: A Prospective Randomized, Controlled Crossover Trial of Autologous Fat Grafting for Rejuvenation of the Heel
Authors: James Isaac B , Gusenoff Beth R , Wang Sheri , DiBernardo Gabriella , Minteer Danielle , Gusenoff Jeffrey A 
Published: February 2021

AbstractBackgroundThe shock-absorbing soft tissues of the heel are composed of dermis and specialized fat pads. Heel fat pad atrophy is common and can be painful and debilitating. In our previous work, autologous fat grafting was effective for treating pain from forefoot fat pad atrophy.ObjectivesThe authors hypothesized that autologous fat grafting to the heel would relieve pain and improve function in patients with heel fat pad atrophy.MethodsPatients with heel fat pad atrophy and associated pain were recruited and randomized into 2 groups. Group 1 received autologous fat grafting on enrollment and was followed for 2 years. Group 2 received offloading and activity modification for 1 year, then crossed over, underwent autologous fat grafting, and was followed for 1 year afterward. Outcome measures included ultrasound-measured fat pad and dermal thickness; pedobarograph-measured foot pressures and forces; and patient-reported outcomes as measured by the Manchester Foot Pain and Disability Index.ResultsThirteen patients met the inclusion criteria and completed the study. Seven (12 affected feet) were randomized into Group 1; and 6 (9 affected feet) were randomized into Group 2. The average age was 55 years and BMI was 30.5 kg/m2. Demographics did not significantly differ between groups. Heel fat pad thickness increased after autologous fat grafting but returned to baseline at 6 months. However, autologous fat grafting increased dermal thickness significantly and also increased fat pad thickness under a compressive load compared with controls at 6 and 12 months. Foot pain, function, and appearance were also significantly improved compared with controls at 6 and 12 months.ConclusionsAutologous fat grafting improved patient-reported foot pain, function, and appearance and may rejuvenate local soft tissues in patients with heel fat pad atrophy.Level of Evidence: 3
Investigating the molecular guts of endoderm formation using zebrafish
Authors: Figiel Daniela M , Elsayed Randa , Nelson Andrew C 
Published: February 2021

AbstractThe vertebrate endoderm makes major contributions to the respiratory and gastrointestinal tracts and all associated organs. Zebrafish and humans share a high degree of genetic homology and strikingly similar endodermal organ systems. Combined with a multitude of experimental advantages, zebrafish are an attractive model organism to study endoderm development and disease. Recent functional genomics studies have shed considerable light on the gene regulatory programs governing early zebrafish endoderm development, while advances in biological and technological approaches stand to further revolutionize our ability to investigate endoderm formation, function and disease. Here, we discuss the present understanding of endoderm specification in zebrafish compared to other vertebrates, how current and emerging methods will allow refined and enhanced analysis of endoderm formation, and how integration with human data will allow modeling of the link between non-coding sequence variants and human disease.
Nine Maxims for the Ecology of Cold-Climate Winters
Authors: Studd Emily K , Bates Amanda E , Bramburger Andrew J , Fernandes Timothy , Hayden Brian , Henry Hugh A L , Humphries Murray M , Martin Rosemary , McMeans Bailey C , Moise Eric R D , O'Sullivan Antóin M , Sharma Sapna , Sinclair Brent J , Sutton Alex O , Templer Pamela H , Cooke Steven J 
Published: February 2021

Abstract
Frozen winters define life at high latitudes and altitudes. However, recent, rapid changes in winter conditions have highlighted our relatively poor understanding of ecosystem function in winter relative to other seasons. Winter ecological processes can affect reproduction, growth, survival, and fitness, whereas processes that occur during other seasons, such as summer production, mediate how organisms fare in winter. As interest grows in winter ecology, there is a need to clearly provide a thought-provoking framework for defining winter and the pathways through which it affects organisms. In the present article, we present nine maxims (concise expressions of a fundamentally held principle or truth) for winter ecology, drawing from the perspectives of scientists with diverse expertise. We describe winter as being frozen, cold, dark, snowy, less productive, variable, and deadly. Therefore, the implications of winter impacts on wildlife are striking for resource managers and conservation practitioners. Our final, overarching maxim, “winter is changing,” is a call to action to address the need for immediate study of the ecological implications of rapidly changing winters.
The current progress and critical analysis of three-dimensional scanning and three-dimensional printing applications in breast surgery
Authors: Alshehri S A , Singh S K , Mosahebi A , Kalaskar D M 
Published: February 2021

Abstract

Background
Several attempts have been made to develop a tool capable of evaluating breast shape and volume to aid surgical planning and outcome assessment. More recently, newer technologies such as three-dimensional (3D) scanning and 3D printing have been applied in breast assessment. The aim of this study was to review the literature to assess the applicability of 3D scanning and 3D printing in breast surgery.

Methods
A literature search was carried on PubMed, Google Scholar and OVID from January 2000 to December 2019 using the keywords ‘3D’, ‘Three-dimensional’, ‘Three/four dimensions’ and ‘Breast’.

Results
A total of 6564 articles were identified initially; the abstracts of 1846 articles were scanned, and 81 articles met the inclusion criteria and were included in this review. Articles were reviewed and classified according to their aims, study subjects, the software and hardware used, main outcomes and major limitations.

Conclusions
These technologies are fast and easy to use, however, high costs, long processing times and the need for training might limit their application. To incorporate these technologies into standard healthcare, their efficacy and effectiveness must be demonstrated through multiple and rigorous clinical trials.
Think Family, Think Relationship: Factors Influencing Stronger Professional Relationships with Parents Diagnosed with a Mental Illness. A Qualitative Study
Authors: Grant A , Devaney J , Davidson G , Lagdon S , Duffy J 
Published: February 2021

Abstract
This qualitative study explored how professionals and parents with mental illness experience their relationships with each other, what aspects of interaction promote a constructive relationship and the role of wider organisational and systemic factors. A purposive sample of 30 adult mental health and children’s services professionals, and 21 parents completed semi-structured interviews. Professionals’ transparent, non-judgemental, empathetic and positive approach and ability to form partnerships and to share power with parents were keys in building trusting relationships with them. Professionals’ capacity to use limited self-disclosure of their own personal experiences (i.e. parenting) enabled them to develop constructive relationships with parents. Equally, important was parents’ willingness to form partnerships with professionals and to accept a whole family approach to service delivery. Professionals’ limited understanding of mental illness and focus on administration hindered their relationships with parents. An understanding of what constitutes a constructive relationship between professionals and parents and how it develops may help professionals to reflect upon how they engage parents and to do it well. It may also assist organisations to develop the necessary structures and resources to create the conditions for promoting constructive engagement between professionals and parents.
Inhibition of Matrix Metalloproteinase 9 Activity Promotes Synaptogenesis in the Hippocampus
Authors: Salamian Ahmad , Legutko Diana , Nowicka Klaudia , Badyra Bogna , Kaźmierska-Grębowska Paulina , Caban Bartosz , Kowalczyk Tomasz , Kaczmarek Leszek , Beroun Anna 
Published: February 2021

Abstract
Information coding in the hippocampus relies on the interplay between various neuronal ensembles. We discovered that the application of a cholinergic agonist, carbachol (Cch), which triggers oscillatory activity in the gamma range, induces the activity of matrix metalloproteinase 9 (MMP-9)—an enzyme necessary for the maintenance of synaptic plasticity. Using electrophysiological recordings in hippocampal organotypic slices, we show that Cch potentiates the frequency of miniature inhibitory and excitatory postsynaptic currents (mIPSCs and mEPSCs, respectively) in CA1 neurons and this effect is MMP-9 dependent. Interestingly, though MMP-9 inhibition prevents the potentiation of inhibitory events, it further boosts the frequency of excitatory mEPSCs. Such enhancement of the frequency of excitatory events is a result of increased synaptogenesis onto CA1 neurons. Thus, the function of MMP-9 in cholinergically induced plasticity in the hippocampus is to maintain the fine-tuned balance between the excitatory and the inhibitory synaptic transmission.
Beta-Alanine and Tris-(hydroxyl methyl) Aminomethane as Peak Modifiers in the Development of RP–HPLC Methods Using Aceclofenac and Haloperidol Hydrochloride as Exemplar Drugs
Authors: Peraman Ramalingam , Chiranjeevi Pokuri , Reddy Yerrigamreddy Padmanabha , Kumar Kondreddy Vinod , Vasantharaju S G , Nagappan Krishnaveni 
Published: February 2021

AbstractIn the present analytical approach, beta-alanine (ALA) and tris-(hydroxyl methyl) aminomethane (TRIS) were investigated as peak modifiers due to their water solubility and their possible peak modifying a property. These reagents were tested for their efficacy on the elution of aceclofenac (ACF) and haloperidol hydrochloride (HLC) from C18 column (250 mm × 4.6 mm, 5 μ) equipped with a photodiode array detector. The test reagents were investigated at 0.25 ± 0.05% concentration with a varying % aqueous composition on elution efficacy of HLC and ACF. The added ALA/TRIS in the mobile phase significantly (P &lt; 0.05) improvised the symmetrical elution of HLC with 3-fold theoretical plates increase (P &lt; 0.05) and 10-fold reduced capacity factor as compared to the control run. For ACF, the shoulder effect observed for ACF peak was eliminated. The optimized mobile phase was a combination of acetonitrile and water containing 0.25% beta-alanine/TRIS (pH 3.5 with ortho-phosphoric acid) at the ratio of 70:30 and 60:40% v/v, respectively, for ACF and HLC. The method was validated as per ICHQ2 guidelines. The column performance was tested for reproducibility in non-peak modifier applications and revealed a null effect on the column, thus these agents are relatively less toxic to HPLC columns.
Reassortant Cache Valley Virus Associated With Acute Febrile, Nonneurologic Illness, Missouri
Authors: Baker Molly , Hughes Holly R , Naqvi S Hasan , Yates Karen , Velez Jason O , McGuirk Sophia , Schroder Barb , Lambert Amy J , Kosoy Olga I , Pue Howard , Turabelidze George , Staples J Erin 
Published: February 2021

Abstract
An adult male from Missouri sought care for fever, fatigue, and gastrointestinal symptoms. He had leukopenia and thrombocytopenia and was treated for a presumed tickborne illness. His condition deteriorated with respiratory and renal failure, lactic acidosis, and hypotension. Next-generation sequencing and phylogenetic analysis identified a reassortant Cache Valley virus.
Potential of eHealth smart technology in optimization and monitoring of heart failure treatment in adults with systemic right ventricular failure
Authors: Nederend Marieke , Zandstra Tjitske E , Kiès Philippine , Jongbloed Monique R M , Vliegen Hubert W , Treskes Roderick W , Schalij Martin J , Atsma Douwe E , Egorova Anastasia D 
Published: February 2021

Abstract

Aims
Patients with a systemic right ventricle (sRV) in the context of transposition of the great arteries (TGA) after atrial switch or congenitally corrected TGA are prone to heart failure and arrhythmias. This study evaluated feasibility, patient adherence, and satisfaction of a smart technology-based care pathway for heart failure treatment optimization in these patients.

Methods and results
Patients with symptomatic sRV failure eligible for initiation of sacubitril/valsartan were provided with four smartphone compatible devices (blood pressure monitor, weight scale, step counter, and rhythm monitor) and were managed according to a smart technology-based care pathway. Biweekly sacubitril/valsartan titration visits were replaced by electronical visits, patients were advised to continue measurements at least weekly after titration. Data of 24 consecutive sRV patients (median age 47 years, 50% female) who participated in the smart technology-based care pathway were analysed. Median home-hospital distance was 65 km (maximum 227 km). Most patients (20, 83.3%) submitted weekly measurements; 100% submitted prior to electronical visits. Titration conventionally occurs during a hospital visit. By implementing eHealth smart technology, 68 such trips to hospital were replaced by virtual visits facilitated by remote monitoring. An eHealth questionnaire was completed by 22 patients (92%), and 96% expressed satisfaction. After titration, 30 instances of remote adjustment of heart failure medication in addition to scheduled outpatient clinic visits occurred, one (4%) heart failure admission followed, despite ambulant adjustments. Five patients (21%) sent in rhythm registrations (n = 17), of these 77% showed sinus rhythm, whereas supraventricular tachycardia was detected in the remaining four registrations.

Conclusion
These data suggest that implementation of a smart technology-based care pathway for optimization of medical treatment sRV failure is feasible with high measurement adherence and patient satisfaction.
Epidemiological transitions in human evolution and the richness of viruses, helminths, and protozoa
Authors: Amoroso Caroline R , Nunn Charles L 
Published: February 2021

Abstract

Background and objectives
In absolute terms, humans are extremely highly parasitized compared to other primates. This may reflect that humans are outliers in traits correlated with parasite richness: population density, geographic range area, and study effort. The high degree of parasitism could also reflect amplified disease risk associated with agriculture and urbanization. Alternatively, controlling for other variables, cultural and psychological adaptations could have reduced parasitism in humans over evolutionary time.

Methodology
We predicted the number of parasites that would infect a nonhuman primate with human phenotypic characteristics and phylogenetic position, and then compared observed parasitism of humans in eight geopolitical countries to the predicted distributions. The analyses incorporated study effort, phylogeny, and drivers of parasitism in 33 primate species.

Results
Analyses of individual countries were not supportive of either hypothesis. When analyzed collectively, however, human populations showed consistently lower than expected richness of protozoa and helminths, but higher richness of viruses. Thus, human evolutionary innovations and new parasite exposures may have impacted groups of parasites in different ways, with support for both hypotheses in the overall analysis.

Conclusions and implications
The high level of parasitism observed in humans only applies to viruses, and was not extreme in any of our tests of individual countries. In contrast, we find consistent reductions in protozoa and helminths across countries, suggesting reduced parasitism by these groups during human evolution. We propose that hygienic and technological advances might have extinguished fecal-orally or indirectly transmitted parasites like helminths, whereas higher human densities and host-shifting potential of viruses have supported increased virus richness.

Lay Summary
Vastly more parasite species infect humans than any other primate host. Controlling for factors that influence parasite richness, such as the intensity of study effort and body mass, we find that humans may have more viruses, but fewer helminths and protozoa, than expected based on evolutionary analyses of parasitism in other primates.
PIP2 Improves Cerebral Blood Flow in a Mouse Model of Alzheimer’s Disease
Authors: Mughal Amreen , Harraz Osama F , Gonzales Albert L , Hill-Eubanks David , Nelson Mark T 
Published: February 2021

Abstract
Alzheimer’s disease (AD) is a leading cause of dementia and a substantial healthcare burden. Despite this, few treatment options are available for controlling AD symptoms. Notably, neuronal activity-dependent increases in cortical cerebral blood flow (CBF; functional hyperemia) are attenuated in AD patients, but the associated pathological mechanisms are not fully understood at the molecular level. A fundamental mechanism underlying functional hyperemia is activation of capillary endothelial inward-rectifying K+ (Kir2.1) channels by neuronally derived potassium (K+), which evokes a retrograde capillary-to-arteriole electrical signal that dilates upstream arterioles, increasing blood delivery to downstream active regions. Here, using a mouse model of familial AD (5xFAD), we tested whether this impairment in functional hyperemia is attributable to reduced activity of capillary Kir2.1 channels. In vivo CBF measurements revealed significant reductions in whisker stimulation (WS)-induced and K+-induced hyperemic responses in 5xFAD mice compared with age-matched controls. Notably, measurements of whole-cell currents in freshly isolated 5xFAD capillary endothelial cells showed that Kir2.1 current density was profoundly reduced, suggesting a defect in Kir2.1 function. Because Kir2.1 activity absolutely depends on binding of phosphatidylinositol 4,5-bisphosphate (PIP2) to the channel, we hypothesized that capillary Kir2.1 channel impairment could be corrected by exogenously supplying PIP2. As predicted, a PIP2 analog restored Kir2.1 current density to control levels. More importantly, systemic administration of PIP2 restored K+-induced CBF increases and WS-induced functional hyperemic responses in 5xFAD mice. Collectively, these data provide evidence that PIP2-mediated restoration of capillary endothelial Kir2.1 function improves neurovascular coupling and CBF in the setting of AD.
Minimally invasive aortic valve replacement: short-term efficacy of sutureless compared with stented bioprostheses
Authors: Paparella Domenico , Santarpino Giuseppe , Moscarelli Marco , Guida Pietro , De Santis Adriano , Fattouch Khalil , Martinelli Luigi , Coppola Roberto , Mikus Elisa , Albertini Alberto , Del Giglio Mauro , Gregorini Renato , Speziale Giuseppe 
Published: February 2021

AbstractOBJECTIVESSutureless aortic valve prostheses have been introduced to facilitate the implant process, speed up the operating time and improve haemodynamic performance. The goal of this study was to assess the potential advantages of using sutureless prostheses during minimally invasive aortic valve replacement in a large multicentre population.METHODSFrom 2011 to 2019, a total of 3402 patients in 11 hospitals underwent isolated aortic valve replacement with minimal access approaches using a bioprosthesis. A total of 475 patients received sutureless valves; 2927 received standard valves. The primary outcome was the incidence of 30-day deaths. Secondary outcomes were the occurrence of major complications following procedures performed with sutureless or standard bioprostheses. Propensity matched comparisons was performed based on a multivariable logistic regression model.RESULTSThe annual number of sutureless valve implants increased over the years. The matching procedure paired 430 sutureless with 860 standard aortic valve replacements. A total of 0.7% and 2.1% patients with sutureless and standard prostheses, respectively, died within 30 days (P = 0.076). Cross-clamp times [48 (40–62) vs 63 min (48–74); P = 0.001] and need for blood transfusions (27.4% vs 33.5%; P = 0.022) were lower in patients with sutureless valves. No difference in permanent pacemaker insertions was observed in the overall population (3.3% vs 4.4% in the standard and sutureless groups; P = 0.221) and in the matched groups (3.6% vs 4.7% in the standard and sutureless groups; P = 0.364).CONCLUSIONSThe use of sutureless prostheses is advantageous and facilitates the adoption of a minimally invasive approach, reducing cardiac arrest time and the number of blood transfusions. No increased risk of permanent pacemaker insertion was observed.
Invisible Images and Indeterminacy: Why We Need a Multi-stage Account of Photography
Authors: Wilson Dawn M 
Published: February 2021

Abstract
Some photographs show determinate features of a scene because the photographed scene had those features. This dependency relation is, rightly, a consensus in philosophy of photography. I seek to refute many long-established theories of photography by arguing that they are incompatible with this commitment. In Section II, I classify accounts of photography as either single-stage or multi-stage. In Section III, I analyze the historical basis for single-stage accounts. In Section IV, I explain why the single-stage view led scientists to postulate “latent” photographic images as a technical phenomenon in early chemical photography. In Section V, I discredit the notion of an invisible latent image in chemical photography and, in Section VI, extend this objection to the legacy of the latent image in digital photography. In Section VII, I appeal to the dependency relation to explain why the notion of a latent image makes the single-stage account untenable. Finally, I use the multi-stage account to advance debate about “new” versus “orthodox” theories of photography.
Population simulations of COVID-19 outbreaks provide tools for risk assessment and continuity planning
Authors: Peng Bo , Pettit Rowland W , Amos Christopher I 
Published: February 2021

Abstract

Objectives
We developed COVID-19 Outbreak Simulator (https://ictr.github.io/covid19-outbreak-simulator/) to quantitatively estimate the effectiveness of preventative and interventive measures to prevent and battle COVID-19 outbreaks for specific populations.

Materials and methods
Our simulator simulates the entire course of infection and transmission of the virus among individuals in heterogeneous populations, subject to operations and influences, such as quarantine, testing, social distancing, and community infection. It provides command-line and Jupyter notebook interfaces and a plugin system for user-defined operations.

Results
The simulator provides quantitative estimates for COVID-19 outbreaks in a variety of scenarios and assists the development of public health policies, risk-reduction operations, and emergency response plans.

Discussion
Our simulator is powerful, flexible, and customizable, although successful applications require realistic estimation and robustness analysis of population-specific parameters.

Conclusion
Risk assessment and continuity planning for COVID-19 outbreaks are crucial for the continued operation of many organizations. Our simulator will be continuously expanded to meet this need.