bluuebunny/crossref_metadata_2025_split huggingface dataset:
Retenção do lítio em solos de áreas adjacentes às impactadas pelo rompimento da barragem do Fundão, Mariana – MG
Authors: Levy Roger , Miranda Fernanda Leal , Firmino Fernando Soares , Marques Rosângela Francisca de Paula Vitor , Thebaldi Michael Silveira , Rocha Marcelo Henrique Fernandes de Faria , Oliveira Alisson Souza de , Santos Claudiomir da Silva dos , Ritá Fabricio dos Santos , Soares Karina de Jesus 
Published: September 2021

Objetivou-se analisar a sorção de Lítio (Li) em solos não contaminados de áreas adjacentes à que recebeu rejeito da Barragem de Fundão, por meio de modelos matemáticos e tempo de contato da solução e solo, visando identificar a potencialidade de contaminação do solo e águas subterrâneas. Coletaram-se amostras deformadas de solo, na profundidade de 0 - 20 cm para caraterização química. Posteriormente pesou-se terra fina seca ao ar e colocou-se em béqueres com 20 mL de cloreto de lítio. As concentrações utilizadas foram baseadas na legislação vigente, para classe 1, sendo: 2,3; 5,2; 10,1; 20,3; 30,1 e 50,0 mg L-1 de Li. Para identificação do melhor tempo de contato entre solução e adsorvente, a solução permaneceu em repouso (1, 12 e 24 horas), à temperatura constante de 20 °C. Os parâmetros das isotermas Linear, Langmuir e Freundlich foram ajustados objetivando obter curvas experimentais da solução. Assim utilizou-se o coeficiente de determinação (R2) e teste F. O solo apresenta baixa capacidade de adsorção devido às características químicas, portanto, vulnerável à contaminação das águas subterrâneas. A maior adsorção foi no tempo de 24 horas e o modelo com melhor ajuste aos dados foi o de Freundlich.
Consequências nutricionais da privação de sono em crianças e adolescentes: uma revisão integrativa
Authors: Oliveira Juliana Paiva de , Ramos Robson Vieira , Sousa Angela Maria de , Oliveira Fabiana Carla Teixeira de , Baldoni Nayara Ragi 
Published: September 2021

O sono é um processo do corpo para regulação metabólica e fisiológica em todos os seres humanos, se tornando necessário tanto em sua quantidade e qualidade. Realizar uma revisão integrativa para analisar as consequências alimentares e nutricionais em crianças e adolescentes que sofrem de privação de sono. Efetuou-se uma busca sistematizada de artigos indexados nas bases de dados: PubMed, Biblioteca Virtual da Saúde (BVS) e Web of Science publicado até a data 03/12/2020. Foram incluídas publicações em todos os idiomas. Dois revisores independentes selecionaram os artigos elegíveis e extraíram os dados dos artigos selecionados. Com base nos 405 artigos encontrados, após aplicação dos critérios de inclusão, 28 artigos foram incluídos. Tendo em vista as consequências da privação de sono, foram encontrados fator de risco para sobrepeso e obesidade, maiores resultados de medidas antropométricas (circunferência da cintura, circunferência do quadril e percentual de gordura corporal), aumento de Índice Massa Corporal, maior consumo calórico, maior ingestão de alimentos industrializados e menor ingestão de frutas. Conclui-se que a privação de sono traz consequências negativas para crianças e adolescentes, tendo como um dos resultados principais o risco para sobrepeso e obesidade, maior ingestão de alimentos industrializados e mais calóricos, menor ingestão de frutas. Diante desses achados, educação em saúde sobre este tema deve ser mais discutida entre os pais e/ou responsáveis de crianças e adolescentes.
Variação das propriedades tecnológicas de painéis MDF em uma linha de produção industrial no Brasil
Authors: Araujo Elesandra da Silva , Protásio Thiago de Paula , Barbosa Antonio Vinicius Correa , Mendes Rafael Farinassi , Guimarães Júnior José Benedito , Mendes Lourival Marin , Silva Marcela Gomes da 
Published: September 2021

No presente estudo foram caracterizadas as propriedades tecnológicas de painéis de fibras de média densidade (MDF), em diferentes horários de produção em uma linha industrial brasileira, com o objetivo de avaliar a variabilidade tecnológica das chapas e sua qualidade em relação à norma técnica comercial. Painéis MDF confeccionados com fibras de Eucalyptus spp. (70%), Schizolobium parahyba (20%) e madeiras residuais de serraria (10%), aglutinados com resina ureia-formaldeído foram coletadas em intervalos de duas horas de um turno de produção da empresa. Foram determinadas às propriedades físicas (umidade, densidade aparente, absorção superficial, absorção de água, inchamento em espessura e taxa de não retorno de espessura) e resistência mecânica (flexão estática, dureza janka, tração perpendicular e arrancamento de parafuso). Os painéis de início e final do turno foram submetidos ao ciclo de envelhecimento acelerado, sendo avaliados posteriormente à densidade, tração e flexão estática. Do total das propriedades avaliadas nos painéis MDF, um percentual de 55% não apresentaram variações entre os horários de produção, e se enquadraram aos valores da norma. Apenas as propriedades de inchamento em espessura (IE 24h) e tração em 80% dos horários, não atenderam aos valores estipulados pela ABNT NBR 15316-1, variando de 11,2 a 15,6% e 0,42 a 0,62 N/mm², respectivamente, entre o início e o final do turno. O envelhecimento acelerado promoveu diminuição expressiva na qualidade das chapas. Os painéis MDF estudados apresentam bom desempenho tecnológico para uso em ambiente seco, e sua variação tecnológica decorreu do uso de fibras de diferentes madeiras na produção das chapas.
Inteligência Artificial no diagnóstico de doenças neurodegenerativas: uma revisão sistemática de literatura
Authors: Brito Emilayne Nicácio Dias , Figueiredo Bárbara Queiroz de , Souto Diego Nunes , Nogueira Júlia Fernandes , Melo Ana Luísa de Castro , Silva Iorrane Tavares da , Oliveira Iuri Pimenta , Almeida Marcelo Gomes de 
Published: September 2021

Introdução: Inteligência Artificial (IA) é um ramo da ciência da computação que se propõe a desenvolver sistemas que simulem a capacidade humana de percepção de um problema, identificando seus componentes para, com isso, resolver problemas e propor/tomar decisões. Objetivo: ampliar conhecimentos e categorizar aplicações do uso da IA para o diagnóstico, tratamento e prognóstico de doenças neurodegenerativas, uma vez que, atualmente, seu uso se torna amplamente aplicável e essencial para contornar as etapas da moléstia. Metodologia: Trata-se de uma pesquisa descritiva do tipo revisão integrativa da literatura realizada através do acesso online nas bases de dados National Library of Medicine (PubMed MEDLINE), Scientific Electronic Library Online (Scielo), Google Scholar, Biblioteca Virtual em Saúde (BVS), Web of Science e EBSCO Information Services, nos meses de junho e julho de 2021. Resultados e discussão: Nos últimos anos, os dados obtidos por redes neurais, aprendizagem profunda e outros métodos matemáticos estão se desenvolvendo a uma velocidade sem precedentes. Eles têm sido amplamente utilizados no campo da análise de imagens, e demostraram grande potencial na análise de imagens médicas no diagnóstico de Doença de Alzheimer, Doença de Parkinson, esclerose múltipla, sendo a aplicação destes métodos podem melhorar ainda mais a capacidade de análise de dados de imagem multimodais complexos e melhorar a eficiência desses diagnósticos. Conclusão: com a inteligência artificial, os distúrbios neurodegenerativos podem ser investigados em um nível mais profundo, fornecendo uma visão geral abrangente da doença e abrindo caminhos para a aplicação da medicina de precisão para essas patologias.
Hubungan Antara Pengetahuan, Sikap dan Praktik Personal Hygiene Organ Genitalia Eksterna Terhadap Kejadian Keputihan Patologis
Authors: Cahyaningtyas Widia A. A. , Nainggolan Sudung , Simanjuntak Tigor P. 
Published: September 2021

Abstrak
Keputihan (leukorea, flour albus, vaginal discharge) adalah sekret berlebihan dari vagina selain darah haid, dan tidak disebabkan neoplasma atau penyakit sistemik. Keputihan merupakan masalah kesehatan reproduksi dialami hampir 75% perempuan didunia, paling tidak sekali selama hidupnya. Kurangnya pengetahuan, sikap, dan praktik dalam perawatan vagina diyakini berpengaruh terhadap kejadian keputihan patologis tersebut. Penelitian ini bertujuan untuk mengetahui hubungan pengetahuan, sikap, dan praktik personal hygiene organ genital eksterna terhadap kejadian keputihan patologis pada mahasiswi Fakultas Kedokteran Universitas Kristen Indonesia. Penelitian ini menggunakan desain penelitian analitik korelasional pada 98 mahasiswi. Terdapathubungan yang bermakna (p= 0,000) dan korelasi positif antara tingkat pengetahuan, sikap dan praktik personal hygiene dengan kejadian keputihan patologis.

Kata Kunci : keputihan, pengetahuan, sikap, praktik, personal hygiene.

Relationship Between Knowledge, Attitudes and Personal Practices of External Genitalia Hygiene Against Pathological Leucorrhoea

Abstract
Vaginal discharge (leukorrhea, flour albus, vaginal discharge) is excessive discharge from the vagina other than menstrual blood, and is not caused by neoplasms or systemic disease. Leucorrhoea is a reproductive health problem that affects nearly 75% of women in the world, at least once in their lifetime. Lack of knowledge, attitude, and practice in vaginal care is believed to have an effect on the incidence of pathological vaginal discharge. This study aims to determine the relationship between knowledge, attitudes, and personal hygiene practices of external genital organs on the incidence of pathological vaginal discharge in students of the Faculty of Medicine, Christian University of Indonesia. This study used a correlational analytical research design on 98 female students of the Faculty of Medicine, Christian University of Indonesia. It was found a significant relationship (p = 0.000) and a positive correlation between the level of knowledge, attitudes and personal hygiene practices with the incidence of pathological vaginal discharge.

Keywords: leucorrhoea, knowledge, attitude, practice, personal hygiene.
State national security policy and intellectual property issues
Authors: Zaikivskyi Oleksandr , Onistrat Oleksandr 
Published: September 2021

Keywords: state policy, national security, intellectual property
National security (“NS”) of Ukraine is achieved through a balancedstate policy in accordance with accepted doctrines, strategies, concepts and programsin such areas as political, economic, social, military, environmental, scientific andtechnological, information, etc.However, many theoretical and practical issues concerning the definition of thecontent, challenges, tasks and mechanisms of implementation of effective functions ofpublic policy and management decisions for the further development of social processesstill remain insufficiently studied. Therefore, the main tasks of the NS system subjectsare constant monitoring of the impact on NS of processes taking place in variousfields (including intellectual property), forecasting, identifying and assessing possiblethreats, destabilizing factors and conflicts, their causes and occurrence consequences.In particular, the impact on NS (especially on defence capabilities) of the significantdevelopment of intellectual property and the full use of intellectual property rights isnot taken into account at all.State NS policy should include measures to prevent the emergence and neutralizationof sources of threats to NS under the influence of the development of intellectualproperty.Theoretical aspects of the state policy of National Security and Defence of Ukraine(“NSDU”) development are considered. The necessity of current problems definition inthis sphere and the inclusion of intellectual property questions in these processes isinvestigated. It is determined that the effective implementation of the state NS policyis impossible without a comprehensive analysis of intellectual property issues impacton the sphere of the NSDU. The importance of further research on the development ofa unified approach to improving the protection of intellectual property and its determinationas a component of NSDU, the development of appropriate recommendationsto address issues of intellectual property in this area and to neutralize sources ofthreats under the influence of intellectual property in the structure of NSDU.
Case Report: BMPR2-Targeted MinION Sequencing as a Tool for Genetic Analysis in Patients With Pulmonary Arterial Hypertension
Authors: Takashima Tomoya , Brisset Sophie , Furukawa Asuka , Taniguchi Hirohisa , Takeyasu Rika , Kawamura Akio , Tamura Yuichi 
Published: September 2021

Background: Mutations in the bone morphogenetic protein receptor type 2 gene (BMPR2) represent a major genetic cause of pulmonary arterial hypertension (PAH). Identification of BMPR2 mutations is crucial for the genetic diagnosis of PAH. MinION nanopore sequencer is a portable third-generation technology that enables long-read sequencing at a low-cost. This nanopore technology-based device has not been used previously for PAH diagnosis. This study aimed to determine the feasibility of using MinION nanopore sequencing for the genetic analysis of PAH patients, focused on BMPR2.Methods: We developed a protocol for the custom bioinformatics pipeline analysis of long reads generated by long-PCR. To evaluate the potential of using MinION sequencing in PAH, we analyzed five samples, including those of two idiopathic PAH patients and a family of three members with one affected patient. Sanger sequencing analysis was performed to validate the variants.Results: The median read length was around 3.4 kb and a good mean quality score of approximately 19 was obtained. The total number of reads generated was uniform among the cases and ranged from 2,268,263 to 3,126,719. The coverage was consistent across flow cells in which the average number of reads per base ranged from 80,375 to 135,603. We identified two polymorphic variants and three mutations in four out of five patients. Certain indel variant calling-related errors were observed, mostly outside coding sequences.Conclusion: We have shown the ability of this portable nanopore sequencer to detect BMPR2 mutations in patients with PAH. The MinION nanopore sequencer is a promising tool for screening BMPR2 mutations, especially in small laboratories and research groups.
Rapid Detection and Quantification of Mycobacterium tuberculosis DNA in Paraffinized Samples by Droplet Digital PCR: A Preliminary Study
Authors: Antonello Maria , Scutari Rossana , Lauricella Calogero , Renica Silvia , Motta Valentina , Torri Stefania , Russo Cristina , Gentile Leonarda , Cento Valeria , Colagrossi Luna , Mattana Giordana , Codecasa Luigi Ruffo , Vismara Chiara , Scaglione Francesco , Veronese Silvio Marco , Bonoldi Emanuela , Bandera Alessandra , Gori Andrea , Mazzola Ester , Perno Carlo Federico , Alteri Claudia 
Published: September 2021

Background: Rapid and reliable diagnosis of tuberculosis (TB) represents a diagnostic challenge in compartmentalized extrapulmonary TB infection because of the small number of mycobacteria (MTB) and the frequent lack of fresh samples to perform culture. Here, we estimate the performances of homemade droplet digital PCR (ddPCR)-based assays against culture in 89 biopsies, for those fresh and formalin-fixed and paraffin-embedded (FFPE) subsamples were available.Methods: MTB diagnosis in fresh subsamples was performed by culture. Fresh subsamples were also analyzed for acid-fast bacilli smear-microscopy (AFB) and Xpert® MTB/RIF (Xpert). MTB examination was repeated in blind in the 89 FFPE subsamples by in-house ddPCR assays targeting the IS6110 and rpoB. Analytical sensitivity of ddPCR assays was evaluated using serial dilution of H37Rv strain. Limit of detection (LOD) was calculated by probit analysis. Results were expressed in copies/106 cells.Results: IS6110 and rpoB ddPCR assays showed a good linear correlation between expected and observed values (R2: 0.9907 and 0.9743, respectively). Probit analyses predicted a LOD of 17 and 40 copies/106 cells of MTB DNA for IS6110 and rpoB, respectively. Of the 89 biopsies, 68 were culture positive and 21 were culture negative. Considering mycobacterial culture as reference method, IS6110 assay yielded positive results in 67/68 culture-positive samples with a median interquartile range (IQR) of 1,680 (550–8,444) copies/106 cells (sensitivity: 98.5%; accuracy: 98.9). These performances were superior to those reported by the rpoB assay in FFPE subsamples (sensitivity: 66.20%; accuracy: 74.1) and even superior to those reported by Xpert and AFB in fresh subsamples (sensitivity: 79.4 and 33.8%, respectively; accuracy: 84.3 and 49.4, respectively). When Xpert and AFB results were stratified according to mycobacterial load detected by rpoB and IS6110 ddPCR, bacterial load was lower in Xpert and AFB negative with respect to Xpert and AFB-positive samples (p = 0.003 and 0.01 for rpoB and p = 0.01 and 0.11 for IS6110), confirming the poor sensitivity of these methods in paucibacillary disease.Conclusion: ddPCR provides highly sensitive, accurate, and rapid MTB diagnosis in FFPE samples, as defined by the high concordance between IS6110 assay and culture results. This approach can be safely introduced in clinical routine to accelerate MTB diagnosis mainly when culture results remain unavailable.
Prominent Striatum Amyloid Retention in Early-Onset Familial Alzheimer's Disease With PSEN1 Mutations: A Pilot PET/MR Study
Authors: Qin Qi , Fu Liping , Wang Ruimin , Lyu Jihui , Ma Huixuan , Zhan Minmin , Zhou Aihong , Wang Fen , Zuo Xiumei , Wei Cuibai 
Published: September 2021

Background: With the advancements of amyloid imaging in recent years, this new imaging diagnostic method has aroused great interest from researchers. Till now, little is known regarding amyloid deposition specialty in patients with early-onset familial Alzheimer's disease (EOFAD), and even less is known about its role in cognitive impairments.Objectives: Our study aimed to evaluate the amyloid deposition in five patients with EOFAD, 15 patients with late-onset sporadic AD, and 12 healthy subjects utilizing 11C-labeled Pittsburgh compound-B (11C-PiB) amyloid PET imaging. Moreover, we figured out the correlation between striatal and cortical standardized uptake value ratios (SUVRs). We also investigated the correlation between 11C-PiB retention and cognitive presentation.Results: All patients with EOFAD showed high amyloid deposition in the striatum, a pattern that is not usually seen in patients with late-onset sporadic AD. The SUVR in the striatum, especially in the amygdala, showed significant correlations with cortex SUVR in EOFAD. However, neither striatal nor cortical 11C-PiB retention was related to cognitive decline.Conclusions: The amyloid distribution in patients with EOFAD differs from late-onset sporadic AD, with higher amyloid deposits in the striatum. Our study also demonstrated positive correlations in 11C-PiB retention between the striatum and other cortical areas. We revealed that the distribution of amyloid in the brain is not random but diffuses following the functional and anatomical connections. However, the degree and pattern of amyloid deposition were not correlated with cognitive deficits.
An Indirect Comparison Between Nivolumab + Ipilimumab + Two Cycles of Chemotherapy vs. Pembrolizumab + Chemotherapy as First-Line Treatment for Metastatic Non-Small Cell Lung Cancer
Authors: Jiang Panpan , Mao Ziyang , Wang Qinyang , Jia Xiaohui , Geng Luying , Xu Hong , Jiang Lili , Yang Chengcheng , Jiao Min , Guo Hui 
Published: September 2021

BackgroundNivolumab + ipilimumab + two cycles chemotherapy (N-I + chemo, intensive immunotherapy but chemo-light) and pembrolizumab + chemotherapy (Pem + chemo) were both recommended as first-line treatment for metastatic non-small cell lung carcinoma (NSCLC) patients. We conducted this indirect comparison to compare the efficacy of and safety between these two treatments for providing reference for decision making.MethodsRelevant databases were searched for eligible trials. A well-accepted adjusted indirect treatment comparison (ITC) approach was selected to pool efficacy results and safety outcomes. Subgroup analyses were stratified according to PD-L1 expression and clinical characteristics.ResultsFour eligible randomized trials (CheckMate9LA, KEYNOTE-021G, KEYNOTE 189, KEYNOTE 407) involving 2017 patients were available to analyze. The ITC results suggested that N-I + chemo is comparable to Pem + chemo in OS (HR 1.03, 95% CI 0.82-1.30) and ORR (RR 0.81, 95% CI 0.62-1.06), but tended to yield inferior PFS (HR 1.28, 95% CI 1.04-1.59) than did Pem + chemo. As for safety profiles, N-I + chemo showed no significant difference relative to Pem + chemo in any grade adverse events: (RR 1.03, 95% CI 0.99-1.10), but demonstrated reduced toxicity in chemo-related adverse events, such as anemia (RR 0.63, 95% CI 0.49-0.81), neutropenia (RR0.51, 95% CI 0.33-0.79), and thrombocytopenia (RR 0.38, 95% CI 0.21-0.69).ConclusionsN-I + chemo is a promising treatment option for providing comparable OS related to Pem + chemo. However, for never smoker female patients, Pem + chemo is preferable to choose for demonstrating favorable OS benefit than N-I + chemo.
High EVI1 Expression Predicts Adverse Outcomes in Children With De Novo Acute Myeloid Leukemia
Authors: Zheng Yongzhi , Huang Yan , Le Shaohua , Zheng Hao , Hua Xueling , Chen Zaisheng , Feng Xiaoqin , Li Chunfu , Zheng Mincui , Xu Honggui , He Yingyi , He Xiangling , Li Jian , Hu Jianda 
Published: September 2021

BackgroundA high ecotropic viral integration site 1 (EVI1) expression (EVI1high) is an independent prognostic factor in adult acute myeloid leukemia (AML). However, little is known of the prognostic value of EVI1high in pediatric AML. This study aimed to examine the biological and prognostic significance of EVI1high in uniformly treated pediatric patients with AML from a large cohort of seven centers in China.MethodsA diagnostic assay was developed to determine the relative EVI1 expression using a single real-time quantitative polymerase chain reaction in 421 newly diagnosed pediatric AML patients younger than 14 years from seven centers in southern China. All patients were treated with a uniform protocol, but only 383 patients were evaluated for their treatment response. The survival data were included in the subsequent analysis (n = 35 for EVI1high, n = 348 for EVI1low).ResultsEVI1high was found in 9.0% of all 421 pediatric patients with de novo AML. EVI1high was predominantly found in acute megakaryoblastic leukemia (FAB M7), MLL rearrangements, and unfavorable cytogenetic aberrance, whereas it was mutually exclusive with t (8; 21), inv (16)/t (16; 16), CEBPA, NPM1, or C-KIT mutations. In the univariate Cox regression analysis, EVI1high had a significantly adverse 5-year event-free survival (EFS) and overall survival (OS) [hazard ratio (HR) = 1.821 and 2.401, p = 0.036 and 0.005, respectively]. In the multivariate Cox regression analysis, EVI1high was an independent prognostic factor for the OS (HR = 2.447, p = 0.015) but not EFS (HR = 1.556, p = 0.174). Furthermore, EVI1high was an independent adverse predictor of the OS and EFS of patients with MLL rearrangements (univariate analysis: HR = 9.921 and 7.253, both p &lt; 0.001; multivariate analysis: HR = 7.186 and 7.315, p = 0.005 and 0.001, respectively). Hematopoietic stem cell transplantation (HSCT) in first complete remission (CR1) provided EVI1high patients with a tendential survival benefit when compared with chemotherapy as a consolidation (5-year EFS: 68.4% vs. 50.8%, p = 0.26; 5-year OS: 65.9% vs. 54.8%, p = 0.45).ConclusionIt could be concluded that EVI1high can be detected in approximately 10% of pediatric AML cases. It is predominantly present in unfavorable cytogenetic subtypes and predicts adverse outcomes. Whether pediatric patients with EVI1high AML can benefit from HSCT in CR1 needs to be researched further.