bluuebunny/crossref_metadata_2025_split huggingface dataset:
A medullary centre for lapping in mice
Authors: Dempsey Bowen , Sungeelee Selvee , Bokiniec Phillip , Chettouh Zoubida , Diem Séverine , Autran Sandra , Harrell Evan R. , Poulet James F. A. , Birchmeier Carmen , Carey Harry , Genovesio Auguste , McMullan Simon , Goridis Christo , Fortin Gilles , Brunet Jean-François 
Published: November 2021

AbstractIt has long been known that orofacial movements for feeding can be triggered, coordinated, and often rhythmically organized at the level of the brainstem, without input from higher centers. We uncover two nuclei that can organize the movements for ingesting fluids in mice. These neuronal groups, IRtPhox2b and Peri5Atoh1, are marked by expression of the pan-autonomic homeobox gene Phox2b and are located, respectively, in the intermediate reticular formation of the medulla and around the motor nucleus of the trigeminal nerve. They are premotor to all jaw-opening and tongue muscles. Stimulation of either, in awake animals, opens the jaw, while IRtPhox2b alone also protracts the tongue. Moreover, stationary stimulation of IRtPhox2b entrains a rhythmic alternation of tongue protraction and retraction, synchronized with jaw opening and closing, that mimics lapping. Finally, fiber photometric recordings show that IRtPhox2b is active during volitional lapping. Our study identifies one of the subcortical nuclei underpinning a stereotyped feeding behavior.
Polluted white dwarfs reveal exotic mantle rock types on exoplanets in our solar neighborhood
Authors: Putirka Keith D. , Xu Siyi 
Published: November 2021

AbstractPrior studies have hypothesized that some polluted white dwarfs record continent-like granitic crust—which is abundant on Earth and perhaps uniquely indicative of plate tectonics. But these inferences derive from only a few elements, none of which define rock type. We thus present the first estimates of rock types on exoplanets that once orbited polluted white dwarfs—stars whose atmospheric compositions record the infall of formerly orbiting planetary objects—examining cases where Mg, Si, Ca and Fe are measured with precision. We find no evidence for continental crust, or other crust types, even after correcting for core formation. However, the silicate mantles of such exoplanets are discernable: one case is Earth like, but most are exotic in composition and mineralogy. Because these exoplanets exceed the compositional spread of >4,000 nearby main sequence stars, their unique silicate compositions are unlikely to reflect variations in parent star compositions. Instead, polluted white dwarfs reveal greater planetary variety in our solar neighborhood than currently appreciated, with consequently unique planetary accretion and differentiation paths that have no direct counterparts in our Solar System. These require new rock classification schemes, for quartz + orthopyroxene and periclase + olivine assemblages, which are proposed here.
Volumetric extrusive rates of silicic supereruptions from the Afro-Arabian large igneous province
Authors: Thines Jennifer E. , Ukstins Ingrid A. , Wall Corey , Schmitz Mark 
Published: November 2021

AbstractThe main phase of silicic volcanism from the Afro-Arabian large igneous province preserves some of the largest volcanic eruptions on Earth, with six units totaling >8,600 km3 dense rock equivalent (DRE). The large volumes of rapidly emplaced individual eruptions present a case study for examining the tempo of voluminous silicic magma generation and emplacement. Here were report high-precision 206Pb/238U zircon ages and show that the largest sequentially dated eruptions occurred within 48 ± 34 kyr (29.755 ± 0.023 Ma to 29.707 ± 0.025 Ma), yielding the highest known long-term volumetric extrusive rate of silicic volcanism on Earth. While these are the largest known sequential silicic supereruptions, they did not cause major global environmental change. We also provide a robust tie-point for calibration of the geomagnetic polarity timescale by integrating 40Ar/39Ar data with our 206Pb/238U ages to yield new constraints on the duration of the C11n.1r Subchron.
Potent SARS-CoV-2 neutralizing antibodies with protective efficacy against newly emerged mutational variants
Authors: Li Tingting , Han Xiaojian , Gu Chenjian , Guo Hangtian , Zhang Huajun , Wang Yingming , Hu Chao , Wang Kai , Liu Fengjiang , Luo Feiyang , Zhang Yanan , Hu Jie , Wang Wang , Li Shenglong , Hao Yanan , Shen Meiying , Huang Jingjing , Long Yingyi , Song Shuyi , Wu Ruixin , Mu Song , Chen Qian , Gao Fengxia , Wang Jianwei , Long Shunhua , Li Luo , Wu Yang , Gao Yan , Xu Wei , Cai Xia , Qu Di , Zhang Zherui , Zhang Hongqing , Li Na , Gao Qingzhu , Zhang Guiji , He Changlong , Wang Wei , Ji Xiaoyun , Tang Ni , Yuan Zhenghong , Xie Youhua , Yang Haitao , Zhang Bo , Huang Ailong , Jin Aishun 
Published: November 2021

AbstractAccumulating mutations in the SARS-CoV-2 Spike (S) protein can increase the possibility of immune escape, challenging the present COVID-19 prophylaxis and clinical interventions. Here, 3 receptor binding domain (RBD) specific monoclonal antibodies (mAbs), 58G6, 510A5 and 13G9, with high neutralizing potency blocking authentic SARS-CoV-2 virus display remarkable efficacy against authentic B.1.351 virus. Surprisingly, structural analysis has revealed that 58G6 and 13G9 both recognize the steric region S470–495 on the RBD, overlapping the E484K mutation presented in B.1.351. Also, 58G6 directly binds to another region S450–458 in the RBD. Significantly, 58G6 and 510A5 both demonstrate prophylactic efficacy against authentic SARS-CoV-2 and B.1.351 viruses in the transgenic mice expressing human ACE2 (hACE2), protecting weight loss and reducing virus loads. Together, we have evidenced 2 potent neutralizing Abs with unique mechanism targeting authentic SARS-CoV-2 mutants, which can be promising candidates to fulfill the urgent needs for the prolonged COVID-19 pandemic.
A previously unrecognized membrane protein in the Rhodobacter sphaeroides LH1-RC photocomplex
Authors: Tani Kazutoshi , Nagashima Kenji V. P. , Kanno Ryo , Kawamura Saki , Kikuchi Riku , Hall Malgorzata , Yu Long-Jiang , Kimura Yukihiro , Madigan Michael T. , Mizoguchi Akira , Humbel Bruno M. , Wang-Otomo Zheng-Yu 
Published: November 2021

AbstractRhodobacter (Rba.) sphaeroides is the most widely used model organism in bacterial photosynthesis. The light-harvesting-reaction center (LH1-RC) core complex of this purple phototroph is characterized by the co-existence of monomeric and dimeric forms, the presence of the protein PufX, and approximately two carotenoids per LH1 αβ-polypeptides. Despite many efforts, structures of the Rba. sphaeroides LH1-RC have not been obtained at high resolutions. Here we report a cryo-EM structure of the monomeric LH1-RC from Rba. sphaeroides strain IL106 at 2.9 Å resolution. The LH1 complex forms a C-shaped structure composed of 14 αβ-polypeptides around the RC with a large ring opening. From the cryo-EM density map, a previously unrecognized integral membrane protein, referred to as protein-U, was identified. Protein-U has a U-shaped conformation near the LH1-ring opening and was annotated as a hypothetical protein in the Rba. sphaeroides genome. Deletion of protein-U resulted in a mutant strain that expressed a much-reduced amount of the dimeric LH1-RC, indicating an important role for protein-U in dimerization of the LH1-RC complex. PufX was located opposite protein-U on the LH1-ring opening, and both its position and conformation differed from that of previous reports of dimeric LH1-RC structures obtained at low-resolution. Twenty-six molecules of the carotenoid spheroidene arranged in two distinct configurations were resolved in the Rba. sphaeroides LH1 and were positioned within the complex to block its channels. Our findings offer an exciting new view of the core photocomplex of Rba. sphaeroides and the connections between structure and function in bacterial photocomplexes in general.
Two distinct superconducting states controlled by orientations of local wrinkles in LiFeAs
Authors: Cao Lu , Liu Wenyao , Li Geng , Dai Guangyang , Zheng Qi , Wang Yuxin , Jiang Kun , Zhu Shiyu , Huang Li , Kong Lingyuan , Yang Fazhi , Wang Xiancheng , Zhou Wu , Lin Xiao , Hu Jiangping , Jin Changqing , Ding Hong , Gao Hong-Jun 
Published: November 2021

AbstractFor iron-based superconductors, the phase diagrams under pressure or strain exhibit emergent phenomena between unconventional superconductivity and other electronic orders, varying in different systems. As a stoichiometric superconductor, LiFeAs has no structure phase transitions or entangled electronic states, which manifests an ideal platform to explore the pressure or strain effect on unconventional superconductivity. Here, we observe two types of superconducting states controlled by orientations of local wrinkles on the surface of LiFeAs. Using scanning tunneling microscopy/spectroscopy, we find type-I wrinkles enlarge the superconducting gaps and enhance the transition temperature, whereas type-II wrinkles significantly suppress the superconducting gaps. The vortices on wrinkles show a C2 symmetry, indicating the strain effects on the wrinkles. By statistics, we find that the two types of wrinkles are categorized by their orientations. Our results demonstrate that the local strain effect with different directions can tune the superconducting order parameter of LiFeAs very differently, suggesting that the band shifting induced by directional pressure may play an important role in iron-based superconductivity.
N1-methyladenosine methylation in tRNA drives liver tumourigenesis by regulating cholesterol metabolism
Authors: Wang Yanying , Wang Jing , Li Xiaoyu , Xiong Xushen , Wang Jianyi , Zhou Ziheng , Zhu Xiaoxiao , Gu Yang , Dominissini Dan , He Lei , Tian Yong , Yi Chengqi , Fan Zusen 
Published: November 2021

AbstractHepatocellular carcinoma (HCC) accounts for the majority of primary liver cancers and is characterized by high recurrence and heterogeneity, yet its mechanism is not well understood. Here we show that N1-methyladenosine methylation (m1A) in tRNA is remarkably elevated in hepatocellular carcinoma (HCC) patient tumour tissues. Moreover, m1A methylation signals are increased in liver cancer stem cells (CSCs) and are negatively correlated with HCC patient survival. TRMT6 and TRMT61A, forming m1A methyltransferase complex, are highly expressed in advanced HCC tumours and are negatively correlated with HCC survival. TRMT6/TRMT61A-mediated m1A methylation is required for liver tumourigenesis. Mechanistically, TRMT6/TRMT61A elevates the m1A methylation in a subset of tRNA to increase PPARδ translation, which in turn triggers cholesterol synthesis to activate Hedgehog signaling, eventually driving self-renewal of liver CSCs and tumourigenesis. Finally, we identify a potent inhibitor against TRMT6/TRMT61A complex that exerts effective therapeutic effect on liver cancer.
Antimicrobial and cytotoxic capacity of pyroligneous extracts films of Eucalyptus grandis and chitosan for oral applications
Authors: de Souza Juliana Leitzke Santos , Alves Tomaz , Camerini Laísa , Nedel Fernanda , Campos Angela Diniz , Lund Rafael Guerra 
Published: November 2021

AbstractChitosan films containing distilled pyroligneous extracts of Eucalyptus grandis (DPEC), characterized and developed by Brazilian Agricultural Research Corporation—Embrapa Temperate Agriculture (EMBRAPA-CPACT), were evaluated for antimicrobial activity against Candida albicans, Streptococcus mutans, and Lactobacillus acidophilus by direct contact test. Further, their capacity for the prevention of teeth enamel demineralization and cytotoxicity in vitro were also determined. The natural polymers were tested at different concentrations (1500–7500 µg mL−1) and the formulation of an experimental fluoride varnish with antimicrobial activity was evaluated by direct contact test, whereas cytotoxicity was analyzed through the colorimetric MTT assay. Preliminary data showed no statistically significant differences in cytotoxicity to NIH/3T3 cell line when DPEC is compared to the control group. On the other hand, the antimicrobial capacity and demineralization effects were found between the test groups at the different concentrations tested. Chitosan films containing distilled pyroligneous extracts of E. grandis may be an effective control strategy to prevent biofilm formation related to dental caries when applied as a protective varnish. They may inhibit the colonization of oral microorganisms and possibly control dental caries through a decrease in pH and impairment of enamel demineralization.
Unveiling microbial preservation under hyperacidic and oxidizing conditions in the Oligocene Rio Tinto deposit
Authors: Fernández-Remolar David C. , Carrizo Daniel , Harir Mourad , Huang Ting , Amils Ricardo , Schmitt-Kopplin Philippe , Sánchez-García Laura , Gomez-Ortiz David , Malmberg Per 
Published: November 2021

AbstractThe preservation of biosignatures on Mars is largely associated with extensive deposits of clays formed under mild early Noachian conditions (> 3.9 Ga). They were followed by widespread precipitation of acidic sulfates considered adverse for biomolecule preservation. In this paper, an exhaustive mass spectrometry investigation of ferric subsurface materials in the Rio Tinto gossan deposit (~ 25 Ma) provides evidence of well-preserved molecular biosignatures under oxidative and acidic conditions. Time of flight secondary ion mass spectrometry (ToF–SIMS) analysis shows a direct association between physical-templating biological structures and molecular biosignatures. This relation implies that the quality of molecular preservation is exceptional and provides information on microbial life formerly operating in the shallow regions of the Rio Tinto subsurface. Consequently, low-pH oxidative environments on Mars could also record molecular information about ancient life in the same way as the Noachian clay-rich deposits.
The effect of age and sex on the expression of GABA signaling components in the human hippocampus and entorhinal cortex
Authors: Ethiraj Jayarjun , Palpagama Thulani Hansika , Turner Clinton , van der Werf Bert , Waldvogel Henry John , Faull Richard Lewis Maxwell , Kwakowsky Andrea 
Published: November 2021

AbstractGamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the nervous system. The GABA signaling system in the brain is comprised of GABA synthesizing enzymes, transporters, GABAA and GABAB receptors (GABAAR and GABABR). Alterations in the expression of these signaling components have been observed in several brain regions throughout aging and between sexes in various animal models. The hippocampus is the memory centre of the brain and is impaired in several age-related disorders. It is composed of two main regions: the Cornu Ammonis (CA1-4) and the Dentate Gyrus (DG), which are interconnected with the Entorhinal Cortex (ECx). The age- and sex-specific changes of GABA signaling components in these regions of the human brain have not been examined. This study is the first to determine the effect of age and sex on the expression of GABA signaling components-GABAAR α1,2,3,5, β1-3, γ2, GABABR R1 and R2 subunits and the GABA synthesizing enzymes GAD 65/67-in the ECx, and the CA1 and DG regions of the human hippocampus using Western blotting. No significant differences were found in GABAAR α1,2,3,5, β1-3, γ2, GABABR R1 and R2 subunit and GAD65/76 expression levels in the ECx, CA1 and DG regions between the younger and older age groups for both sexes. However, we observed a significant negative correlation between age and GABAAR α1subunit level in the CA1 region for females; significant negative correlation between age and GABAAR β1, β3 and γ2 subunit expression in the DG region for males. In females a significant positive correlation was found between age and GABAAR γ2 subunit expression in the ECx and GABABR R2 subunit expression in the CA1 region. The results indicate that age and sex do not affect the expression of GAD 65/67. In conclusion, our results show age- and sex-related GABAA/BR subunit alterations in the ECx and hippocampus that might significantly influence GABAergic neurotransmission and underlie disease susceptibility and progression.
Circulation patterns of human seasonal Influenza A viruses in Chile before H1N1pdm09 pandemic
Authors: Mena Juan , Tapia Rodrigo , Verdugo Claudio , Avendaño Luis , Parra-Castro Paulina , Medina Rafael A. , Barriga Gonzalo , Neira Víctor 
Published: November 2021

AbstractUnderstanding the diversity and circulation dynamics of seasonal influenza viruses is key to public health decision-making. The limited genetic information of pre-pandemic seasonal IAVs in Chile has made it difficult to accurately reconstruct the phylogenetic relationships of these viruses within the country. The objective of this study was to determine the genetic diversity of pre-pandemic human seasonal IAVs in Chile. We sequenced the complete genome of 42 historic IAV obtained between 1996 and 2007. The phylogeny was determined using HA sequences and complemented using other segments. Time-scale phylogenetic analyses revealed that the diversity of pre-pandemic human seasonal IAVs in Chile was influenced by continuous introductions of new A/H1N1 and A/H3N2 lineages and constant viral exchange between Chile and other countries every year. These results provide important knowledge about genetic diversity and evolutionary patterns of pre-pandemic human seasonal IAVs in Chile, which can help design optimal surveillance systems and prevention strategies. However, future studies with current sequences should be conducted.
β2-adrenergic receptor regulates ER-mitochondria contacts
Authors: Lim Youngshin , Cho Il-Taeg , Rennke Helmut G. , Cho Ginam 
Published: November 2021

AbstractInteractions between the endoplasmic reticulum (ER) and mitochondria (Mito) are crucial for many cellular functions, and their interaction levels change dynamically depending on the cellular environment. Little is known about how the interactions between these organelles are regulated within the cell. Here we screened a compound library to identify chemical modulators for ER-Mito contacts in HEK293T cells. Multiple agonists of G-protein coupled receptors (GPCRs), beta-adrenergic receptors (β-ARs) in particular, scored in this screen. Analyses in multiple orthogonal assays validated that β2-AR activation promotes physical and functional interactions between the two organelles. Furthermore, we have elucidated potential downstream effectors mediating β2-AR-induced ER-Mito contacts. Together our study identifies β2-AR signaling as an important regulatory pathway for ER-Mito coupling and highlights the role of these contacts in responding to physiological demands or stresses.
Distance between the center of the FAZ measured automatically and the highest foveal bulge using OCT-angiography in elderly healthy eyes
Authors: Shoji Takuhei , Ishii Hirokazu , Kanno Junji , Sasaki Takanori , Yoshikawa Yuji , Ibuki Hisashi , Shinoda Kei 
Published: November 2021

AbstractThe center of the fovea, termed the foveola, is the area of highest visual acuity, has the highest density of cone photoreceptors. We investigated the distance between the automatically-determined center of the foveal avascular zone (FAZ) and the manually-determined highest foveal bulge (FB) point using single swept-source optical coherence tomography angiography (OCTA) instrument. This cross-sectional study included 49 eyes of 49 individuals (34 women and 15 men; median age: 68 years) with no history of ocular disorders. The FAZ in the superficial capillary plexus was automatically determined using the Kanno–Saitama macro method, and the center of the FAZ was automatically determined using ellipse approximation. Another candidate foveal center, the highest FB point, was determined manually on the serial cross-sectional B-scan images. As a result, the foveal center was manually identified as the highest FB point on B-scan OCTA images. The center of the FAZ was more likely to be located inferior to the highest FB point (p = 0.031). In participants with a total (linear) distance of more than 50 μm between the center of the FAZ and the highest FB point, the displacement was significantly more in the horizontal direction than in the vertical direction (p = 0.017). These results can be applicable to further studies regarding the spatial relationships between the center of the FAZ and the highest FB point in various macular diseases or previously-treated eyes.
The immune checkpoint VISTA exhibits high expression levels in human gliomas and associates with a poor prognosis
Authors: Ghouzlani Amina , Lakhdar Abdelhakim , Rafii Soumaya , Karkouri Mehdi , Badou Abdallah 
Published: November 2021

AbstractIn human gliomas, anti-tumor T cell responses are inhibited through induction of local and systemic immunosuppression. Immune checkpoint blockade is proving to be a success in several types of cancers. However, many studies reported that the treatment of glioblastoma patients with anti-CTLA-4 or anti-PD-1 has no survival benefit compared to standard chemotherapy. This study aimed to investigate the expression and role of VISTA, a newly described immune checkpoint regulator, in human gliomas. mRNA expression was assessed in a total of 87 samples from glioma patients. 57 glioma tissues were taken at different grades. 20 peripheral blood mononuclear cells (PBMC) samples were taken before surgery and ten after surgery, all from the same set of patients. As for the control, ten specimens of PBMC were taken from healthy donors. Protein expression using immunohistochemistry was performed for 30 patients. The Cancer Genome Atlas (TCGA) data set, was also used to investigate VISTA expression through analysis of RNA-seq data of 667 glioma patients. In the Moroccan cohort, VISTA gene expression was significantly upregulated in glioma tissues related to PBMC of healthy donors. This high expression was specific to patient tissues since VISTA expression in PBMC was low when assessed either before or after surgery. Besides, VISTA exhibited higher expression levels in grade III/IV relative to grade I/II glioma patients. Interestingly, VISTA correlated positively with PD-1 expression. PD-1 also showed elevated expressions in higher glioma grades. The TCGA cohort corroborated these observations. Indeed, VISTA was also found to be strongly expressed in high grades. It was positively correlated with other critical immune checkpoints. Finally, increased VISTA transcript levels were associated with weak overall survival of glioma patients. Our study highlighted a correlation between high levels of VISTA expression and poor prognosis in glioma patients. VISTA might be involved in glioma progression and could be considered as a possible new therapeutic target, especially in advanced gliomas.