bluuebunny/crossref_metadata_2025_split huggingface dataset:
Spatial dynamics and vaccine-induced fitness changes of Bordetella pertussis
Authors: Lefrancq Noémie , Bouchez Valérie , Fernandes Nadia , Barkoff Alex-Mikael , Bosch Thijs , Dalby Tine , Åkerlund Thomas , Fabianova Katerina , Vestrheim Didrik F. , Fry Norman K. , González-López Juan José , Gullsby Karolina , Habington Adele , He Qiushui , Litt David , Martini Helena , Piérard Denis , Stefanelli Paola , Stegger Marc , Zavadilova Jana , Armatys Nathalie , Landier Annie , Guillot Sophie , Hong Samuel L. , Lemey Philippe , Parkhill Julian , Toubiana Julie , Cauchemez Simon , Salje Henrik , Brisse Sylvain 
Published: November 2021

ABSTRACTCompetitive interactions between pathogen strains drive infection risk. Vaccines are thought to perturb strain diversity through shifts in immune pressures, however, this has rarely been measured due to inadequate data and analytical tools. Bordetella pertussis (B. pertussis), responsible for 160,000 deaths annually1, provides a rare natural experiment as many countries have switched from whole cell vaccines to acellular vaccines, which have very different immunogenic properties2,3. Here we use 3,344 sequences from 23 countries and build phylogenetic models to reveal that B. pertussis has substantial diversity within communities, with the relative fitness of local genotypes changing in response to switches in vaccine policy. We demonstrate that the number of transmission chains circulating within subnational regions is strongly associated with host population size. It takes 5-10 years for individual lineages to be homogeneously distributed throughout Europe or the United States. Increased fitness of pertactin-deficient strains following implementation of acellular vaccines, but reduced fitness otherwise, can explain long-term genotype dynamics. These findings highlight the role of national vaccine policies in shifting local diversity of a pathogen that still poses a large burden on global public health.
Transcriptional regulation of cyclophilin D by BMP/SMAD signaling and its role in osteogenic differentiation
Authors: Sautchuk Rubens , Kalicharan Brianna H , Escalera-Rivera Katherine , Jonason Jennifer H , Porter George A , Awad Hani , Eliseev Roman A 
Published: November 2021

SUMMARYCyclophilin D (CypD) promotes opening of the mitochondrial permeability transition pore (MPTP) which plays a key role in both cell physiology and pathology. It is, therefore beneficial for cells to tightly regulate CypD and MPTP but little is known about such regulation. We have reported before that CypD is downregulated and MPTP deactivated during differentiation in various tissues. Herein, we identify BMP/Smad signaling, a major driver of differentiation, as a transcriptional repressor of the CypD gene, Ppif. Using osteogenic induction of mesenchymal lineage cells as a model of BMP/Smad-dependent differentiation, we show that CypD is in fact transcriptionally repressed during this process. The importance of such CypD downregulation is evidenced by the negative effect of CypD ‘rescue’ via gain-of-function on osteogenesis both in vitro and in vivo. In sum, we characterized BMP/Smad signaling as a regulator of CypD expression and elucidated the role of CypD downregulation during cell differentiation.
Distance-dependent inhibition of translation initiation by downstream out-of-frame AUGs reveals that ribosome scans in a Brownian ratchet process
Authors: Li Ke , Kong Jinhui , Zhang Shuo , Zhao Tong , Qian Wenfeng 
Published: November 2021

AbstractWhile eukaryotic ribosomes are widely presumed to scan mRNA for the AUG codon to initiate translation in a strictly 5′–3′ movement (strictly unidirectional scanning model), other evidence has suggested that the ribosome uses small-amplitude 5′–3′ and 3′–5′ oscillations with a net 5′–3′ movement to recognize the AUG codon (Brownian ratchet scanning model). Here, we generated 13,437 yeast variants, each with an ATG triplet placed downstream (dATGs) of the annotated ATG (aATG) codon of green fluorescent protein. We found that out-of-frame dATGs could inhibit translation at the aATG, but with diminishing strength over increasing distance between aATG and dATG, undetectable beyond ∼17 nt. Computational simulations revealed that each triplet is scanned back and forth approximately ∼10 times until an AUG codon is recognized. Collectively, our findings uncover the basic process by which eukaryotic ribosomes scan for initiation codons, and how this process could shape eukaryotic genome evolution and influence cancer development.
An essential ER-resident N-acetyltransferase in Plasmodium falciparum
Authors: Polino Alexander J , Floyd Katherine , Avila-Cruz Yolotzin , Yang Yujuan , Goldberg Daniel E. 
Published: November 2021

AbstractN-terminal acetylation is a common eukaryotic protein modification that involves the addition of an acetyl group to the N-terminus of a polypeptide. This modification is largely performed by cytosolic N-terminal acetyltransferases (NATs). Most associate with the ribosome, acetylating nascent polypeptides co-translationally. In the malaria parasite Plasmodium falciparum, exported effectors are translated into the ER, processed by the aspartic protease plasmepsin V and then N-acetylated, despite having no clear access to cytosolic NATs. Here, we used post-transcriptional knockdown to investigate the most obvious candidate, Pf3D7_1437000. We found that it co-localizes with the ER-resident plasmepsin V and is required for parasite growth. However, depletion of Pf3D7_1437000 had no effect on protein export or acetylation of the exported proteins HRP2 and HRP3. Pf3D7_1437000-depleted parasites arrested later in their development cycle than export-blocked parasites, suggesting the protein’s essential role is distinct from protein export.
High-Speed Imaging Reveals the Bimodal Nature of Dense Core Vesicle Exocytosis
Authors: Zhang Pengcheng , Rumschitzki David , Edwards Robert H. 
Published: November 2021

ABSTRACTDuring exocytosis, the fusion of secretory vesicle with plasma membrane forms a pore that regulates release of neurotransmitter and peptide. Heterogeneity of fusion pore behavior has been attributed to stochastic variation in a common exocytic mechanism, implying a lack of biological control. Using a fluorescent false neurotransmitter (FFN), we imaged dense core vesicle (DCV) exocytosis in primary mouse adrenal chromaffin cells at millisecond resolution and observed strikingly divergent modes of release, with fast events lasting <30 ms and slow events persisting for seconds. Dual imaging of slow events shows a delay in the entry of external dye relative to FFN release, suggesting exclusion by an extremely narrow pore <1 nm in diameter. Unbiased comprehensive analysis shows that the observed variation cannot be explained by stochasticity alone, but rather involve distinct mechanisms, revealing the bimodal nature of DCV exocytosis. Further, loss of calcium sensor synaptotagmin 7 increases the proportion of slow events without changing the intrinsic properties of either class, indicating the potential for independent regulation. The identification of two distinct mechanisms for release capable of independent regulation suggests a biological basis for the diversity of fusion pore behavior.
Religiosity and stigmatization related to mental illness among African-Americans and Black immigrants: cross-sectional observational study and moderation analysis
Authors: Pederson Aderonke Bamgbose , Earnshaw Valerie A. , Lewis-Fernández Roberto , Hawkins Devan , Mangale Dorothy I. , Tsai Alexander C. , Thornicroft Graham 
Published: November 2021

AbstractObjectivesStigma about mental illness is a known barrier to engagement in mental health services. This study aimed to estimate the associations between religiosity and mental illness stigma among Black adults.DesignWe conducted an online cross-sectional study of Black adults in the United States (n=269, ages 18-65) from diverse ethnic backgrounds.ResultsMost (n=248 [92%]) participants attended religious services; while 21 (8%) never attended. Social distance was assessed as an index of past or current stigmatizing behavior. After adjusting for demographic factors, respondents with higher attendance at religious services or greater engagement in religious activities (e.g., prayer, meditation or Bible study) reported greater proximity to people living with mental health problems (RR=1.72; 95% CI: 1.14, 2.59 and RR=1.82; CI: 1.18, 2.79, respectively). Despite reporting greater past or current social proximity, respondents with higher religiosity indices also reported greater future intended stigmatizing behavior (or lower future intended social proximity) (RR’s=0.92-0.98). Ethnicity moderated the association between religiosity and future intended stigmatizing behavior. Black immigrants with higher religiosity reported lower future intended stigmatizing behavior (RR=1.16 CI: 1.02 – 1.32) whereas African-Americans with higher religiosity reported greater future intended stigmatizing behavior (RR = 0.83 CI: 0.76, 0.91).ConclusionsHigher indices of religiosity were associated with lower past or current stigmatizing behavior towards individuals living with mental health problems but not lower future intended stigmatizing behavior. Focusing specifically on future intended stigmatizing behavior and the respondent’s level of religiosity, age, and ethnicity may be critical for designing effective stigma-reducing interventions for Black adults.
Plasmids as donors of Insertion Sequence elements mediating colistin resistance in Klebsiella pneumoniae
Authors: Fordham Stephen , Mantzouratou Anna , Sheridan Elizabeth Anne 
Published: November 2021

AbstractColistin is a last resort antibiotic for the treatment of carbapenemase producing Klebsiella pneumoniae (CRKP). In line with rising colistin use worldwide, colistin resistant Klebsiella pneumoniae (K. pneumoniae) isolates have emerged. The disruption of mgrB by insertion sequences (ISs) has been widely reported worldwide representing a mechanism mediating colistin resistance. Evidence suggests plasmids encode mobilizable IS elements which preferentially integrate into the mgrB gene in K. pneumoniae causing gene inactivation and colistin resistance.Recognised IS elements targeting mgrB include ISL3 (ISKpn25), IS5 (ISKpn26), ISKpn14 and IS903B-like elements. K. pneumoniae represents the single largest species carrying plasmids encoding these IS elements. For IS presence among species, 1000 BLASTn hits were downloaded and filtered for plasmids. Additionally, the top 120 BLASTn non-duplicate circularised plasmid contig hits for each IS element were typed for incompatibility (Inc) group and carbapenemase gene presence.IS903B was found in 28 unique Inc groups, while ISKpn25 was largely carried by IncFIB(pQil) plasmids. ISKpn26 and ISKpn14 were most often found associated with IncFII(pHN7A8) plasmids. Of the 34 unique countries which contained any of the IS elements, ISKpn25 was identified from 26. ISKpn26, ISKpn14, and IS903B insertion sequences were identified from 89.3%, 44.9% and 23.9% plasmid samples from China.Plasmids carrying ISKpn25, ISKpn14, and ISKpn26 IS elements are 12.18, 27.0, and 44.43 times more likely to carry carbapenemase genes relative to plasmids carrying the IS element IS903B. Moreover, plasmids carrying ISKpn26, ISKpn25, and ISKpn14 were 6.10, 28.82, and 31.47 times more likely to be sourced from a clinical environmental setting than the environment relative to IS903B IS harboring plasmid.ISKpn25 present on IncFIB(pQil) sourced from clinical settings is established across multiple countries, while ISKpn26, ISKpn14, and IS903B appear most often in China. High carbapenemase presence in tandem with IS elements may help promote an extensively drug resistant profile in K. pneumoniae limiting already narrow therapeutic treatment options.
Haspin participates in Aurora phosphorylation at centromeres and contributes to chromosome congression in male mouse meiosis
Authors: Berenguer I , López-Jiménez P , Mena I , Viera A , Page J , Maestre C , Malumbres M , Suja JA , Gómez R 
Published: November 2021

ABSTRACTChromosome segregation requires that centromeres properly attach to spindle microtubules. This is an essential step towards the accuracy of cell division and therefore must be precisely regulated in both mitosis and meiosis. One of the main centromeric regulatory signaling pathways is the Haspin-H3T3ph-chromosomal passenger complex (CPC) cascade, which is responsible for the recruitment of the CPC to the centromeres. In mitosis, Haspin kinase phosphorylates H3 at threonine 3 (H3T3ph), the essential histone mark that recruits the CPC whose catalytic component is Aurora B kinase. To date, no data has yet been presented about the action of the centromeric Haspin-H3T3ph-CPC pathway in mammalian male meiosis. We have analyzed the consequences of Haspin chemical inhibition in cultured spermatocytes using LDN-192960. Ourin vitrostudies suggest that Haspin kinase activity is required for proper chromosome congression during both meiotic divisions and for the recruitment of phosphorylated Aurora B at meiotic centromeres. These results have been confirmed by the characterization of the meiotic phenotype of the genetic mouse modelHaspin-/-, which displays similar defects. In addition, our work demonstrates that the absence of H3T3ph histone mark does not alter SGO2 localization to meiotic centromeres. These results add new and relevant information regarding the regulation of centromere function during meiosis.
Factors influencing community participation in the implementation and monitoring of FLEGT-VPA in Ghana
Authors: Derkyi Mercy Afua Adutwumwaa , Appau Yaw , Boakye Boadu Kwadwo 
Published: November 2021

PurposeVoluntary Partnership Agreement (VPA) offers a framework for open and participatory forest management for forest actors, particularly communities fringing forest reserves. This paper aimed to determine the factors influencing the participation of local communities in the implementation and monitoring of Forest Law Enforcement, Governance and Trade-Voluntary Partnership Agreement (FLEGT-VPA) activities.Design/methodology/approachThe study was conducted in three communities in Goaso Forest District in the Ahafo Region of Ghana. Based on a cross-sectional design, 105 (n = 105) community members were interviewed using a semi-structured questionnaire. Logistic regression analysis was performed to identify socio-demographic and cultural factors that influence people's participation in FLEGT-VPA activities. Friedman and Wilcoxon signed-rank tests were conducted to analyse the influence of the environmental role of the forest and participation in FLEGT-VPA activities.FindingsThe study found a significant association between the leadership status of respondents' local customs/taboo days and participation in both implementation and monitoring activities. At the same time, a significant association was found between respondents' participation in previous training programs and FLEGT-VPA monitoring activities. The environmental role of the forest was also found to have a significant association with respondents' participation in FLEGT-VPA activities.Originality/valueStudies on the factors that influence the participation of forest fringe communities in Ghana in implementing and monitoring FLEGT-VPA activities are non-existent or scarce. This study identified significant socio-demographic and environmental factors contributing to participatory forest conservation modules such as FLEGT-VPA which will guide future forest conservation initiatives that are inclusive of stakeholders’ interests/concerns.
After-hours work-related technology use and individuals' deviance: the role of other-initiated versus self-initiated interruptions
Authors: Khalid Junaid , Weng Qingxiong Derek , Luqman Adeel , Rasheed Muhammad Imran , Hina Maryam 
Published: November 2021

PurposeThe purpose of this study is to investigate the effect of after-hours work-related technology use on interpersonal, organizational and nonwork deviance through work–family conflict (WFC) by focusing on the moderating role of other- and self-initiated interruptions.Design/methodology/approachThe online survey included 318 valid samples from employees working in different organizations in the Anhui provinces of the People's Republic of China. The authors applied Statistical Package for Social Sciences (SPSS) process macros for testing mediation and moderated mediation analysis while conducting path analytic procedures and bootstrapping analysis.FindingsThis study found that after-hours work-related technology use is positively associated with interpersonal, organizational and nonwork deviance through WFC. This positive relationship strengthens in the presence of other-initiated interruptions compared with self-initiated interruptions. The results show that as compared to self-initiated interruptions, other-initiated interruptions strengthen the relationship between after-hours work-related technology use and its outcomes in the forms of WFC and deviance.Originality/valueAfter-hours work-related technology use is a ubiquitous phenomenon and got significant scholarly attention. However, its effect on WFC and individual deviant behaviors has never been studied. Moreover, the moderated-mediation role of self-initiated and other-initiated interruptions presents a unique and important development in the context of after-hours work-related technology use and deviant behavior.
Sustainable recovery for digital entrepreneurs with shared resources: enablers, challenges and solutions
Authors: Jha Ajay , Sindhwani Rohit , Dwivedi Ashish , Saddikuti Venkataramanaiah 
Published: November 2021

Purpose
The purpose of this study is to identify important criteria for sustainable recovery of digital entrepreneurship from distress situation using shared resources. During pandemic disruption, the importance of sharing economy in managing business efficiency is reflected through this research.

Design/methodology/approach
The present study advances the knowledge on shared resources in business by integrating case study approach with multi criteria decision-making (MCDM) model. A fuzzy analytic hierarchy process approach is adopted to compute criteria weights, and a fuzzy technique for order performance by similarity to ideal solution (TOPSIS) technique is used to rank the sharing economy entrepreneurial ventures during COVID-19 pandemic in the context of emerging economy.

Findings
The present study identified five most important enablers (technological innovation, technology expertise, convergence of virtual and physical spaces, collaboration rather than competition, and benefits to underserved groups through transparency) for sustainable recovery of sharing economy ventures in emerging economy. For example, the study highlights online tutoring through shared intellect as the most sought after sharing economy venture during pandemic disruption, which fulfills the identified enablers.

Practical implications
The proposed framework provides an accurate decision support tool to rank the various identified potential enablers of sharing economy during disruptions. Further, the approach is practically relevant to sharing economy entrepreneurs in selecting the best approach to recover sustainability during pandemic.

Originality/value
The study is unique in addressing the need of sustainability for digital ventures via sharing economy approach in emerging economy (India). To develop a conceptual framework, the present study incorporates a case based approach together with the hybrid MCDM model. Further, the extant literature on disruptions is enhanced by prioritizing the enablers for sharing economy during pandemic.
Were the US workers in “essential” sectors well paid?
Authors: Ou Yi-Pey , Chu Yun-Peng 
Published: November 2021

PurposeWhen the coronavirus disease 2019 (COVID-19) pandemic seriously hit the USA, a lot of cities/states announced their lockdowns, in some cases forbidding employees to go to work. But workers in the so called “essential sectors” were exempt from the order, and on the contrary were required to remain on the job in order to maintain the services and functions considered vital to the community. If they have not been paid well in comparison to those in the other sectors, there would be a stronger case for granting them a special hazard pay during the pandemic. This paper aims to design a way to measure the “importance” or being “essential” of the different sectors in the economy, and then investigates whether the actual pay of the workers in these sectors is consistent with the measured importance.Design/methodology/approachAt least two policy issues emerged from such an arrangement: (1) How can one define the “essential sectors” objectively instead of the authorities preparing a list according to their administrative procedure? (2) How well have been the workers in the essential sectors paid before the pandemic strike? The concept of a revised Leontief forward linkage effect will be used in an input–output model to gauge the relative “importance” of the different sectors in the US economy. Then the measured importance will be compared with the average compensation of the employees in these sectors.FindingsIt is found that for some sectors such as agriculture, retail trade, and repair and installation of machinery and equipment the ratio of workers' compensation relative to the national average is substantially lower than the relative importance of the sectors employing them. That is, many of them have been substantially underpaid in spite of their importance.Research limitations/implicationsThe scope of this study is limited to one country, the USA, but the methodology can be applied to other countries as well.Originality/valueThis study is an original research that contributes to an improved understanding of the importance of the workers engaged in different sectors in the USA during COVID-19.