bluuebunny/crossref_metadata_2025_split huggingface dataset:
Therapeutic Effect of IL1β Priming Tonsil Derived-Mesenchymal Stem Cells in Osteoporosis
Authors: Yoo Minjoo , Cho Sungkuk , Shin Sunhye , Kim Jung-Mi , Park Hyeon-Gyeong , Cho Sungyoo , Hwang Yu Kyeong , Park Dae Hwi 
Published: June 2021

Abstract
Background:
Stem cell therapies can be a new therapeutic strategy that may rebalance anabolic and anti-resorptive effects in osteoporosis patients. Tonsil-derived mesenchymal stem cells (TMSCs) can be an alternative therapeutic source for chronic degenerative diseases including osteoporosis. MSCs acquire immune regulatory function under the inflammatory cytokines. Since interleukin (IL) 1β is known to be one of inflammatory cytokines involved in osteoporosis progression, treatment of IL1β with TMSCs may enhance immunomodulatory function and therapeutic effects of TMSCs in osteoporosis.

Methods:
For IL1β priming, TMSCs were cultured in the presence of the medium containing IL1β for 1 day. Characteristics of IL1β priming TMSCs such as multipotent differentiation properties, anti-inflammatory potential, and suppression of osteoclast differentiation were assessed in vitro. For in vivo efficacy study, IL1β priming TMSCs were intravenously infused twice with ovariectomized (OVX) osteoporosis mouse model, and blood serum and bone parameters from micro computed tomography images were analyzed.

Results:
IL1β priming TMSCs had an enhanced osteogenic differentiation and secreted factors that regulate both osteoclastogenesis and osteoblastogenesis. IL1β priming TMSCs also suppressed proliferation of peripheral blood mononuclear cells (PBMCs) and decreased expression of Receptor activator of nuclear factor kappa-Β ligand (RANKL) in PHA-stimulated PBMCs. Furthermore, osteoclast specific genes such as Nuclear factor of activated T cells c1 (NFATc1) were effectively down regulated when co-cultured with IL1β priming TMSCs in RANKL induced osteoclasts. In OVX mice, IL1β priming TMSCs induced low level of serum RANKL/osteoprotegerin (OPG) ratio on the first day of the last administration. Four weeks after the last administration, bone mineral density and serum Gla-osteocalcin were increased in IL1β priming TMSC-treated OVX mice. Furthermore, bone formation and bone resorption markers that had been decreased in OVX mice with low calcium diet were recovered by infusion of IL1β priming TMSCs.

Conclusion:
IL1β priming can endow constant therapeutic efficacy with TMSCs, which may contribute to improve bone density and maintain bone homeostasis in postmenopausal osteoporosis. Therefore, IL1β priming TMSCs can be a new therapeutic option for treating postmenopausal osteoporosis.
Smart Bionic Surfaces with Switchable Wettability and Applications
Authors: Li Shuyi , Fan Yuyan , Liu Yan , Niu Shichao , Han Zhiwu , Ren Luquan 
Published: June 2021

AbstractIn order to satisfy the needs of different applications and more complex intelligent devices, smart control of surface wettability will be necessary and desirable, which gradually become a hot spot and focus in the field of interface wetting. Herein, we review interfacial wetting states related to switchable wettability on superwettable materials, including several classical wetting models and liquid adhesive behaviors based on the surface of natural creatures with special wettability. This review mainly focuses on the recent developments of the smart surfaces with switchable wettability and the corresponding regulatory mechanisms under external stimuli, which is mainly governed by the transformation of surface chemical composition and geometrical structures. Among that, various external stimuli such as physical stimulation (temperature, light, electric, magnetic, mechanical stress), chemical stimulation (pH, ion, solvent) and dual or multi-triggered stimulation have been sought out to realize the regulation of surface wettability. Moreover, we also summarize the applications of smart surfaces in different fields, such as oil/water separation, programmable transportation, anti-biofouling, detection and delivery, smart soft robotic etc. Furthermore, current limitations and future perspective in the development of smart wetting surfaces are also given. This review aims to offer deep insights into the recent developments and responsive mechanisms in smart biomimetic surfaces with switchable wettability under external various stimuli, so as to provide a guidance for the design of smart surfaces and expand the scope of both fundamental research and practical applications.
Actuation of Liquid Flow by Guided Acoustic Waves on Punched Steel Tapes with Protruding Loops
Authors: Backer Alexander , Landskron Johannes , Drese Klaus Stefan , Lindner Gerhard 
Published: June 2021

AbstractIn a biomimetic approach the feasibility of liquid flow actuation by vibrating protruding structures excited via guided acoustic waves is investigated. Inspired by periodically beating cilia the loop part of a punched metallic hook-and-loop tape with tilted protruding loops was used as a waveguide for plate waves in water. Such waves were excited in the frequency range of 110 Hz to 220 Hz by directly coupling the tape to a loudspeaker membrane. A flow generated in the tilt direction of the loops with velocities up to 60 mm·s−1 was visualized by ink droplets deposited on the tape. The phenomenon persisted, when the protruding length of the loops was reduced by decreasing the protrusion angle. However, after closing the punch holes near the loops with sticking tape streaming could not be observed any longer. The same happened with open punch holes when the ink was replaced by glycerol. Low-frequency acoustic streaming around vibrating sharp edges is proposed as an explanation for the observed phenomena. Applications are expected with respect to the modification of flow profiles and the enhancement of transport processes along and across liquid-solid boundaries.
RNAi of vacuolar-type H+-ATPase genes causes growth delay and molting defect inHenosepilachna vigintioctopunctata
Authors: Zeng Jie , Mu Li-Li , Jin Lin , Ali Anjum Ahmad , Li Guo-Qing 
Published: June 2021

AbstractHenosepilachna vigintioctopunctatais one of the most serious insect pests to a large number of nightshades and cucurbits. RNA interference (RNAi) triggered by double-stranded RNA (dsRNA) offers a reduced risk approach to control the beetle. Identification of amenable target genes and determination of appropriate life stage for dsRNA treatment are two critical steps in order to improve RNAi efficiency. In the present paper, we identified three vATPase genes, namelyHvvATPaseC,HvvATPaseEandHvvATPaseH. We found that the three transcripts were widely expressed in the eggs, first- to fourth-instar larvae, prepupae, pupae and adults. They were abundantly transcribed in the hindgut and Malpighian tubules, in contrast to the epidermis and fat body. Three days' ingestion of dsvATPaseC, dsvATPaseEand dsvATPaseHby the fourth-instar larvae significantly decreased corresponding transcript level by 90.1, 88.9 and 97.2%, greatly reduced larval fresh weight by 28.0, 29.9 and 28.0%, and caused 66.7, 100 and 78.7% larval lethality respectively. Comparably, 3 days' exposure of the third-instar larvae to dsvATPaseCsignificantly reducedHvvATPaseCmRNA level by 89.5%, decreased approximately 80% of the larval fresh weight, and killed 100% of the treated larvae. Therefore, the three vATPase genes, especiallyHvvATPaseE, are potential amenable target genes and young larvae are more susceptible to dsRNA. Our findings will enable the development of the dsRNA-based pesticide to controlH. vigintioctopunctata.
Intravascular ultrasonography-guided measurement and transcatheter closure of highly calcified patent ductus arteriosus in an older adult
Authors: Kawai Yoko , Nakamura Takeshi , Matoba Satoaki 
Published: June 2021

AbstractIn older adults with patent ductus arteriosus, CT is widely used for duct visualisation because angiography images can be inadequate for evaluation. We report the case of a 73-year-old woman with a highly calcified patent ductus arteriosus whose CT images were insufficient for accurate measurement. Intravascular ultrasonography is useful for sizing of and guiding device closure of the duct.
Fishy SPS Measures? The WTO's Korea – Radionuclides Dispute
Authors: Brewster Rachel , Fischer Carolyn 
Published: June 2021

AbstractThe Korea–Radionuclides case addresses Korean SPS measures imposed on Japanese fishery products after the Fukushima Dai-ichi nuclear plant meltdown in 2011. Japan challenged these measures as more restrictive than necessary under the SPS Agreement. The panel agreed with Japan, but this ruling was largely reversed by the Appellate Body. Korea's victory at the Appellate Body was based on procedure. The panel accepted Korea's appropriate level of protection (ALOP), which included both quantitative and qualitative elements. However, the Appellate Body found that the panel only addressed the quantitative aspect of Korea's ALOP and reversed on that basis. The Appellate Body's ruling did not affirmatively find that Korea's SPS measures were legal under WTO rules. Instead, the Appellate Body found that panel had not sufficiently addressed Korea's arguments and, thereby, the panel could not determine that the SPS measures were more restrictive than necessary. The case highlights the need for the Appellate Body to be able to conduct its own factual analysis, a power it could be given if the dispute settlement system is reformed. Without independent fact-finding power, the Appellate Body cannot correct panels’ mistakes, and respondents can prevail based on panel error.
Acquisition and Online Display of High‐Resolution Backscattered Electron and X‐Ray Maps of Meteorite Sections
Authors: Ogliore Ryan C. 
Published: June 2021

AbstractAnalyses of meteorites in thin or thick section begins with a detailed mineralogic/petrologic study of the sample. Backscattered electron and X‐ray imaging in a scanning electron microscope is critical for the characterization and study of the meteorite sections at sub‐μm to cm size scales. Here, I describe techniques to acquire backscattered electron and X‐ray images of an entire one‐inch thin or thick section at high resolution, assemble large mosaic mosaic maps of the data, and display the maps conveniently online in a web browser. The code to acquire, stitch, and display the maps is made available as an open‐source project on GitHub.
NK-/T-cell lymphomas
Authors: Wang Hua , Fu Bi-bo , Gale Robert Peter , Liang Yang 
Published: June 2021

AbstractNatural killer/T-cell lymphoma (NKTL) is a sub-type of Epstein–Barr virus (EBV)-related non-Hodgkin lymphomas common in Asia and Latin America but rare elsewhere. Its pathogenesis is complex and incompletely understood. Lymphoma cells are transformed from NK- or T-cells, sometimes both. EBV-infection and subsequent genetic alterations in infected cells are central to NKTL development. Hemophagocytic syndrome is a common complication. Accurate staging is important to predict outcomes but there is controversy which system is best. More than two-thirds of NKTL lympohmas are localized at diagnosis, are frequently treated with radiation therapy only and have 5-year survival of about 70 percent. Persons with advanced NKTLs receive radiation therapy synchronously or metachronously with diverse multi-drug chemotherapy typically includingl-asparginase with 5-year survival of about 40 percent. Some persons with widespread NKTL receive chemotherapy only. There are few data on safety and efficacy of high-dose therapy and a haematopoietic cell autotransplant. Immune therapies, histone deacetylase (HDAC)-inhibitors and other drugs are in early clinical trials. There are few randomized controlled clinical trials in NKTLs and no therapy strategy is clearlybest; more effective therapy(ies) are needed. Some consensus recommendations are not convincingly evidence-based. Mechanisms of multi-drug resistance are considered. We discuss these issues including recent advances in our understanding of and therapy of NKTLs.
Avian extremity reconstruction via osseointegrated leg-prosthesis for intuitive embodiment
Authors: Hochgeschurz Sarah , Bergmeister Konstantin D. , Brånemark Rickard , Aman Martin , Rocchi Attillio , Restitutti Flavia , Gumpenberger Michaela , Sporer Matthias E. , Gstoettner Clemens , Kramer Anne-Margarete , Lang Susanna , Podesser Bruno K. , Aszmann Oskar C. 
Published: June 2021

AbstractFor large avians such as vultures, limb loss leads to loss of ambulation and eventually death from malnutrition. Prosthetic devices may replace the limb, however, conventional prosthetic sockets are not feasible in feathered limbs and the extreme stress and strain of unreflected daily use in animals. Osseointegration is a novel technique, where external prosthetic parts are connected directly to a bone anchor to provide a solid skeletal-attachment. This concept provides a high degree of embodiment since osseoperception will provide direct intuitive feedback allowing natural use of the limb in gait and feeding. Here we demonstrate for the first time an osseointegrated bionic reconstruction of a limb in a vulture after a tarsometatarsal amputation with a longterm follow-up.
Selective control of the contact and transport between droplet pairs by electrowetting-on-dielectric for droplet-array sandwiching technology
Authors: Konishi Satoshi , Ohya Chikara , Yamada Tatsuhiro 
Published: June 2021

AbstractMethodological advances in on-chip technology enable high-throughput drug screening, such as droplet-array sandwiching technology. Droplet-array sandwiching technology involves upper and lower substrates with a droplet-array designed for a one-step process. This technology is, however, limited to batch manipulation of the droplet-array. Here, we propose a method for selective control of individual droplets, which allows different conditions for individual droplet pairs. Electrowetting-on-dielectric (EWOD) technology is introduced to control the height of the droplets so that the contact between droplet-pairs can be individually controlled. Circular patterns 4 mm in diameter composed of electrodes for EWOD and hydrophilic–hydrophobic patterns for droplet formation 4 μl in volume were developed. We demonstrate the selective control of the droplet height by EWOD for an applied voltage up to 160 V and selective control of the contact and transport of substances. Presented results will provide useful method for advanced drug screening, including cell-based screening.
Multiple functions of reversine on the biological characteristics of sheep fibroblasts
Authors: Guo Yu , Zhu Huan , Li Xiangchen , Ma Caiyun , Sun Tingting , Wang Yuanyuan , Wang Chunjing , Guan Weijun , Liu Changqing 
Published: June 2021

AbstractPrevious reports have demonstrated that Reversine can reverse differentiation of lineage-committed cells to mesenchymal stem cells and suppress tumors growth. However, the molecular mechanisms of antitumor activity and promoting cellular dedifferentiation for reversine have not yet been clearly elucidated. In the present study, it was demonstrated that reversine of 5 μM could induce multinucleated cells through cytokinesis failure rather than just arrested in G2 or M phase. Moreover, reversine reversed the differentiation of sheep fibroblasts into MSC-like style, and notably increased the expression of pluripotent marker genes Oct4 and MSCs-related surface antigens. The fibroblasts treated with reversine could transdifferentiate into all three germ layers cells in vitro. Most importantly, the induced β-like cells and hepatocytes had similar metabolic functions with normal cells in vivo. In addition, reversine promoted fibroblasts autophagy, ROS accumulation, mitochondrial dysfunction and cell apoptosis via the mitochondria mediated intrinsic pathway. The results of high-throughput RNA sequencing showed that most differentially expressed genes (DEGs) involved in Mismatch repair, Nucleotide excision repair and Base excision repair were significantly up-regulated in reversine treated fibroblasts, which means that high concentration of reversine will cause DNA damage and activate the DNA repair mechanism. In summary, reversine can increase the plasticity of sheep fibroblasts and suppress cell growth via the mitochondria mediated intrinsic pathway.
Uncovering of cytochrome P450 anatomy by SecStrAnnotator
Authors: Midlik Adam , Navrátilová Veronika , Moturu Taraka Ramji , Koča Jaroslav , Svobodová Radka , Berka Karel 
Published: June 2021

AbstractProtein structural families are groups of homologous proteins defined by the organization of secondary structure elements (SSEs). Nowadays, many families contain vast numbers of structures, and the SSEs can help to orient within them. Communities around specific protein families have even developed specialized SSE annotations, always assigning the same name to the equivalent SSEs in homologous proteins. A detailed analysis of the groups of equivalent SSEs provides an overview of the studied family and enriches the analysis of any particular protein at hand. We developed a workflow for the analysis of the secondary structure anatomy of a protein family. We applied this analysis to the model family of cytochromes P450 (CYPs)—a family of important biotransformation enzymes with a community-wide used SSE annotation. We report the occurrence, typical length and amino acid sequence for the equivalent SSE groups, the conservation/variability of these properties and relationship to the substrate recognition sites. We also suggest a generic residue numbering scheme for the CYP family. Comparing the bacterial and eukaryotic part of the family highlights the significant differences and reveals a well-known anomalous group of bacterial CYPs with some typically eukaryotic features. Our workflow for SSE annotation for CYP and other families can be freely used at addresshttps://sestra.ncbr.muni.cz.
Loss of the transcriptional repressor Rev-erbα upregulates metabolism and proliferation in cultured mouse embryonic fibroblasts
Authors: Gillis Sean P. , Yao Hongwei , Rizal Salu , Maeda Hajime , Chang Julia , Dennery Phyllis A. 
Published: June 2021

AbstractThe transcriptional repressor Rev-erbα is known to down-regulate fatty acid metabolism and gluconeogenesis gene expression. In animal models, disruption of Rev-erbα results in global changes in exercise performance, oxidative capacity, and blood glucose levels. However, the complete extent to which Rev-erbα-mediated transcriptional repression of metabolism impacts cell function remains unknown. We hypothesized that loss of Rev-erbα in a mouse embryonic fibroblast (MEF) model would result in global changes in metabolism. MEFs lacking Rev-erbα exhibited a hypermetabolic phenotype, demonstrating increased levels of glycolysis and oxidative phosphorylation. Rev-erbα deletion increased expression of hexokinase II, transketolase, and ribose-5-phosphate isomerase genes involved in glycolysis and the pentose phosphate pathway (PPP), and these effects were not mediated by the transcriptional activator BMAL1. Upregulation of oxidative phosphorylation was not accompanied by an increase in mitochondrial biogenesis or numbers. Rev-erbα repressed proliferation via glycolysis, but not the PPP. When treated with H2O2, cell viability was reduced in Rev-erbα knockout MEFs, accompanied by increased ratio of oxidized/reduced NADPH, suggesting that perturbation of the PPP reduces capacity to mount an antioxidant defense. These findings uncover novel mechanisms by which glycolysis and the PPP are modulated through Rev-erbα, and provide new insights into how Rev-erbα impacts proliferation.
Evaluation of the prognostic value of CBXs in gastric cancer patients
Authors: He Mengya , Yue Limin , Wang Haiyan , Yu Feiyan , Yu Mingyang , Ni Peng , Zhang Ke , Chen Shuaiyin , Duan Guangcai , Zhang Rongguang 
Published: June 2021

AbstractChromobox (CBX) proteins were suggested to exert epigenetic regulatory and transcriptionally repressing effects on target genes and might play key roles in the carcinogenesis of a variety of carcinomas. Nevertheless, the functions and prognostic significance of CBXs in gastric cancer (GC) remain unclear. The current study investigated the roles of CBXs in the prognosis of GC using the Oncomine, The Gene Expression Profiling Interactive Analysis (GEPIA), UALCAN, The Cancer Genome Atlas (TCGA), and cBioPortal databases. CBX1/2/3/4/5 were significantly upregulated in GC tissues compared with normal tissues, and CBX7 was downregulated. Multivariate analysis showed that high mRNA expression levels of CBX3/8 were independent prognostic factors for prolonged OS in GC patients. In addition, the genetic mutation rate of CBXs was 37% in GC patients, and genetic alterations in CBXs showed no association with OS or disease-free survival (DFS) in GC patients. These results indicated that CBX3/8 can be prognostic biomarkers for the survival of GC patients.
Assessment of a complete and classified platelet proteome from genome-wide transcripts of human platelets and megakaryocytes covering platelet functions
Authors: Huang Jingnan , Swieringa Frauke , Solari Fiorella A. , Provenzale Isabella , Grassi Luigi , De Simone Ilaria , Baaten Constance C. F. M. J. , Cavill Rachel , Sickmann Albert , Frontini Mattia , Heemskerk Johan W. M. 
Published: June 2021

AbstractNovel platelet and megakaryocyte transcriptome analysis allows prediction of the full or theoretical proteome of a representative human platelet. Here, we integrated the established platelet proteomes from six cohorts of healthy subjects, encompassing 5.2 k proteins, with two novel genome-wide transcriptomes (57.8 k mRNAs). For 14.8 k protein-coding transcripts, we assigned the proteins to 21 UniProt-based classes, based on their preferential intracellular localization and presumed function. This classified transcriptome-proteome profile of platelets revealed: (i) Absence of 37.2 k genome-wide transcripts. (ii) High quantitative similarity of platelet and megakaryocyte transcriptomes (R = 0.75) for 14.8 k protein-coding genes, but not for 3.8 k RNA genes or 1.9 k pseudogenes (R = 0.43–0.54), suggesting redistribution of mRNAs upon platelet shedding from megakaryocytes. (iii) Copy numbers of 3.5 k proteins that were restricted in size by the corresponding transcript levels (iv) Near complete coverage of identified proteins in the relevant transcriptome (log2fpkm > 0.20) except for plasma-derived secretory proteins, pointing to adhesion and uptake of such proteins. (v) Underrepresentation in the identified proteome of nuclear-related, membrane and signaling proteins, as well proteins with low-level transcripts. We then constructed a prediction model, based on protein function, transcript level and (peri)nuclear localization, and calculated the achievable proteome at ~ 10 k proteins. Model validation identified 1.0 k additional proteins in the predicted classes. Network and database analysis revealed the presence of 2.4 k proteins with a possible role in thrombosis and hemostasis, and 138 proteins linked to platelet-related disorders. This genome-wide platelet transcriptome and (non)identified proteome database thus provides a scaffold for discovering the roles of unknown platelet proteins in health and disease.