bluuebunny/crossref_metadata_2025_split huggingface dataset:
Monotonous driving induces shifts in spatial attention as a function of handedness
Authors: Chandrakumar D. , Coussens S. , Keage H. A. D. , Banks S. , Dorrian J. , Loetscher T. 
Published: May 2021

AbstractCurrent evidence suggests that the ability to detect and react to information under lowered alertness conditions might be more impaired on the left than the right side of space. This evidence derives mainly from right-handers being assessed in computer and paper-and-pencil spatial attention tasks. However, there are suggestions that left-handers might show impairments on the opposite (right) side compared to right-handers with lowered alertness, and it is unclear whether the impairments observed in the computer tasks have any real-world implications for activities such as driving. The current study investigated the alertness and spatial attention relationship under simulated monotonous driving in left- and right-handers. Twenty left-handed and 22 right-handed participants (15 males, mean age = 23.6 years, SD = 5.0 years) were assessed on a simulated driving task (lasting approximately 60 min) to induce a time-on-task effect. The driving task involved responding to stimuli appearing at six different horizontal locations on the screen, whilst driving in a 50 km/h zone. Decreases in alertness and driving performance were evident with time-on-task in both handedness groups. We found handedness impacts reacting to lateral stimuli differently with time-on-task: right-handers reacted slower to the leftmost stimuli, while left-handers showed the opposite pattern (although not statistically significant) in the second compared to first half of the drive. Our findings support suggestions that handedness modulates the spatial attention and alertness interactions. The interactions were observed in a simulated driving task which calls for further research to understand the safety implications of these interactions for activities such as driving.
Ancient genomes provide insights into family structure and the heredity of social status in the early Bronze Age of southeastern Europe
Authors: Žegarac A. , Winkelbach L. , Blöcher J. , Diekmann Y. , Krečković Gavrilović M. , Porčić M. , Stojković B. , Milašinović L. , Schreiber M. , Wegmann D. , Veeramah K. R. , Stefanović S. , Burger J. 
Published: May 2021

AbstractTwenty-four palaeogenomes from Mokrin, a major Early Bronze Age necropolis in southeastern Europe, were sequenced to analyse kinship between individuals and to better understand prehistoric social organization. 15 investigated individuals were involved in genetic relationships of varying degrees. The Mokrin sample resembles a genetically unstructured population, suggesting that the community’s social hierarchies were not accompanied by strict marriage barriers. We find evidence for female exogamy but no indications for strict patrilocality. Individual status differences at Mokrin, as indicated by grave goods, support the inference that females could inherit status, but could not transmit status to all their sons. We further show that sons had the possibility to acquire status during their lifetimes, but not necessarily to inherit it. Taken together, these findings suggest that Southeastern Europe in the Early Bronze Age had a significantly different family and social structure than Late Neolithic and Early Bronze Age societies of Central Europe.
Application of multiple omics and network projection analyses to drug repositioning for pathogenic mosquito-borne viruses
Authors: Amemiya Takayuki , Horimoto Katsuhisa , Fukui Kazuhiko 
Published: May 2021

AbstractPathogenic mosquito-borne viruses are a serious public health issue in tropical and subtropical regions and are increasingly becoming a problem in other climate zones. Drug repositioning is a rapid, pharmaco-economic approach that can be used to identify compounds that target these neglected tropical diseases. We have applied a computational drug repositioning method to five mosquito-borne viral infections: dengue virus (DENV), zika virus (ZIKV), West Nile virus (WNV), Japanese encephalitis virus (JEV) and Chikungunya virus (CHIV). We identified signature molecules and pathways for each virus infection based on omics analyses, and determined 77 drug candidates and 146 proteins for those diseases by using a filtering method. Based on the omics analyses, we analyzed the relationship among drugs, target proteins and the five viruses by projecting the signature molecules onto a human protein–protein interaction network. We have classified the drug candidates according to the degree of target proteins in the protein–protein interaction network for the five infectious diseases.
Segmenting surface boundaries using luminance cues
Authors: DiMattina Christopher , Baker Curtis L. 
Published: May 2021

AbstractSegmenting scenes into distinct surfaces is a basic visual perception task, and luminance differences between adjacent surfaces often provide an important segmentation cue. However, mean luminance differences between two surfaces may exist without any sharp change in albedo at their boundary, but rather from differences in the proportion of small light and dark areas within each surface, e.g. texture elements, which we refer to as a luminance texture boundary. Here we investigate the performance of human observers segmenting luminance texture boundaries. We demonstrate that a simple model involving a single stage of filtering cannot explain observer performance, unless it incorporates contrast normalization. Performing additional experiments in which observers segment luminance texture boundaries while ignoring super-imposed luminance step boundaries, we demonstrate that the one-stage model, even with contrast normalization, cannot explain performance. We then present a Filter–Rectify–Filter model positing two cascaded stages of filtering, which fits our data well, and explains observers' ability to segment luminance texture boundary stimuli in the presence of interfering luminance step boundaries. We propose that such computations may be useful for boundary segmentation in natural scenes, where shadows often give rise to luminance step edges which do not correspond to surface boundaries.
Effect of various weight loss interventions on serum NT-proBNP concentration in severe obese subjects without clinical manifest heart failure
Authors: Hollstein Tim , Schlicht Kristina , Krause Laura , Hagen Stefanie , Rohmann Nathalie , Schulte Dominik M. , Türk Kathrin , Beckmann Alexia , Ahrens Markus , Franke Andre , Schreiber Stefan , Becker Thomas , Beckmann Jan , Laudes Matthias 
Published: May 2021

AbstractObesity is associated with a “natriuretic handicap” indicated by reduced N-terminal fragment of proBNP (NT-proBNP) concentration. While gastric bypass surgery improves the natriuretic handicap, it is presently unclear if sleeve gastrectomy exhibits similar effects. We examined NT-proBNP serum concentration in n = 72 obese participants without heart failure before and 6 months after sleeve gastrectomy (n = 28), gastric bypass surgery (n = 19), and 3-month 800 kcal/day very-low calorie diet (n = 25). A significant weight loss was observed in all intervention groups. Within 6 months, NT-proBNP concentration tended to increase by a median of 44.3 pg/mL in the sleeve gastrectomy group (p = 0.07), while it remained unchanged in the other groups (all p ≥ 0.50). To gain insights into potential effectors, we additionally analyzed NT-proBNP serum concentration in n = 387 individuals with different metabolic phenotypes. Here, higher NT-proBNP levels were associated with lower nutritional fat and protein but not with carbohydrate intake. Of interest, NT-proBNP serum concentrations were inversely correlated with fasting glucose concentration in euglycemic individuals but not in individuals with prediabetes or type 2 diabetes. In conclusion, sleeve gastrectomy tended to increase NT-proBNP levels in obese individuals and might improve the obesity-associated “natriuretic handicap”. Thereby, nutritional fat and protein intake and the individual glucose homeostasis might be metabolic determinants of NT-proBNP serum concentration.
A human infertility-associated KASH5 variant promotes mitochondrial localization
Authors: Bentebbal Sana A. , Meqbel Bakhita R. , Salter Anna , Allan Victoria , Burke Brian , Horn Henning F. 
Published: May 2021

AbstractKASH5 is the most recently identified member of the KASH domain family of tail anchored, outer nuclear membrane (ONM) and endoplasmic reticulum (ER) proteins. During meiosis prophase I, KASH5 and SUN1 form a complex that spans the nuclear envelope and which links the telomeres of meiotic chromosomes to cytoplasmic dynein. This connection is essential for homologous chromosome dynamics and pairing. A recent study identified a variant in human KASH5 (L535Q) that correlated with male infertility associated with azoospermia. However, no molecular mechanism was described. Here, we report that this amino acid substitution, within the KASH5 transmembrane domain (TMD) has no predicted effects on secondary structure. However, the overall hydrophobicity of the L535Q TMD, is calculated to be lower than thewild-typeKASH5, based on the GES (Goldman–Engelman–Steitz) amino acid hydrophobicity scale. This change in hydrophobicity profoundly affects the subcellular localization of KASH5. Through a series of amino acid substitution studies, we show that the L535Q substitution perturbs KASH5 localization to the ER and ONM and instead results in mistargeting to the mitochondria membrane. We suggest that this mislocalization accounts for the infertility and azoospermia phenotype in patients.
Typhoon storm surge in the southeast Chinese mainland modulated by ENSO
Authors: Feng Xingru , Li Mingjie , Li Yuanlong , Yu Fujiang , Yang Dezhou , Gao Guandong , Xu Lingjing , Yin Baoshu 
Published: May 2021

AbstractIn the past decade (2010–2019), the annual maximum typhoon storm surge (AMTSS) accounted for 46.6% of the total direct economic loss caused by marine disasters in Chinese mainland, but its prediction in advance is challenging. By analyzing records of 23 tide-gauge stations, we found that the AMTSSs in Shanghai, Zhejiang and Fujian show significant positive correlations with the El Niño-Southern Oscillation (ENSO). For the 1987–2016 period, the maximum correlation is achieved at Pingtan station, where correlation coefficient between the AMTSS and Niño-3.4 is 0.55. The AMTSS occurring in El Niño years are stronger than those in non-El Niño years by 9–35 cm in these areas. Further analysis suggests that a developing El Niño can greatly modulate the behaviors of Northwest Pacific typhoons. Strong typhoons tend to make landfall in southeast China with stronger intensities and northward shifted landfall positions. This study indicates that the modulation effect by ENSO may provide potential predictability for the AMTSS, which is useful for the early alert and reduction of storm surge damages.
Distribution of adenylyl cyclase/cAMP phosphodiesterase gene, CAPE, in streptophytes reproducing via motile sperm
Authors: Yamamoto Chiaki , Takahashi Fumio , Ooe Yosuke , Shirahata Haruto , Shibata Aika , Kasahara Masahiro 
Published: May 2021

AbstractWe recently isolated a novel adenylyl cyclase/cAMP phosphodiesterase gene from the liverwort, Marchantia polymorpha. The protein encoded by this gene has a class III adenylyl cyclase (AC) in the C-terminal domain and class I phosphodiesterase (PDE) in the N-terminal domain; therefore, we named it CAPE (COMBINED AC with PDE). CAPE protein is likely involved in spermatogenesis and sperm motility due to its tissue-specific expression pattern in M. polymorpha and the distribution of CAPE genes in streptophytes. However, little is known about the distribution of CAPE in gymnosperms that use motile sperm for fertilization, such as cycads and ginkgo. The present study aimed to isolate CAPE genes from the cycad, Cycas revoluta, the ginkgo, Ginkgo biloba, and the hornwort, Anthoceros agerestis. Sequences with high homology to CAPE were obtained from these species. Our analyses revealed that all plant taxonomic groups reproducing via motile sperm possessed CAPE, whereas those that do not produce motile sperm did not possess CAPE, with one exception in gymnosperm Cupressales. The phylogenic distribution of CAPE almost corresponds to the evolutionary history of motile sperm production and further suggests that CAPE may be involved in sexual reproduction process using motile sperm in streptophytes.
Body temperature and esthesia in individuals with stroke
Authors: da Silva Dias Caren , Alfieri Fábio Marcon , dos Santos Artur Cesar Aquino , Battistella Linamara Rizzo 
Published: May 2021

AbstractPatients with sequelae of stroke commonly report somatosensory losses. It is believed that body temperature may be associated with tactile sensibility and sensorimotor recovery of these patients. Demonstrate the associations among tactile sensibility, cutaneous temperature, subjective temperature perception, and sensorimotor recovery of patients with stroke sequelae. 86 patients with stroke sequelae were included. Patients had standardized regions of interest (ROIs) assessed with infrared thermography (FLIR T650SC) and monofilaments esthesiometry, and global motor recovery was evaluated with Fugl-Meyer Assessment (FMA). The presence of self-reported perception of temperature difference was used to divide the participants into two groups of 43 patients, and correlation tests were applied to establish correlations among variables. There is no clinically relevant association between tactile sensibility and cutaneous temperature of the foot, regardless of the subjective sensation of temperature changes. Sensorimotor recovery evaluated by FMA is associated with the difference of sensibility between both sides of the body (p < 0.001), as well as with the difference of tactile sensibility (p < 0.001). A clinically significant association between the difference of cutaneous temperature and tactile sensibility was not found, regardless of the presence or absence of subjective perception of such temperature difference. However, sensorimotor recovery is correlated with cutaneous temperature differences and tactile sensibility.
Characterizing the interactions between classical and community-aware centrality measures in complex networks
Authors: Rajeh Stephany , Savonnet Marinette , Leclercq Eric , Cherifi Hocine 
Published: May 2021

AbstractIdentifying vital nodes in networks exhibiting a community structure is a fundamental issue. Indeed, community structure is one of the main properties of real-world networks. Recent works have shown that community-aware centrality measures compare favorably with classical measures agnostic about this ubiquitous property. Nonetheless, there is no clear consensus about how they relate and in which situation it is better to use a classical or a community-aware centrality measure. To this end, in this paper, we perform an extensive investigation to get a better understanding of the relationship between classical and community-aware centrality measures reported in the literature. Experiments use artificial networks with controlled community structure properties and a large sample of real-world networks originating from various domains. Results indicate that the stronger the community structure, the more appropriate the community-aware centrality measures. Furthermore, variations of the degree and community size distribution parameters do not affect the results. Finally, network transitivity and community structure strength are the most significant drivers controlling the interactions between classical and community-aware centrality measures.
Supplementation of Lactobacillus early in life alters attention bias to threat in piglets
Authors: Verbeek Else , Dicksved Johan , Keeling Linda 
Published: May 2021

AbstractGut microbes play an important role in regulating brain processes and influence behaviour, cognition and emotional states in humans and rodents. Nevertheless, it is not known how ingestion of beneficial microbes modulates emotional states in piglets and whether it can improve welfare. Here we use an attention bias task to assess the effects of Lactobacillus reuteri ATCC-PTA-6475 and Lactobacillus plantarum L1-6 supplementation early in life on emotional states in 33 piglets compared to 31 placebo supplemented piglets. We hypothesized that Lactobacillus supplementation would reduce vigilance behaviour (head at shoulder height or higher) and attention (head oriented towards the threat) in response to an auditory threat. The results showed that the control group increased vigilance behaviour in response to the threat, but there was no increase in the probiotics group. Despite the increased vigilance, the control group paid less attention to the threat. One explanation may be that control piglets avoided looking in the direction of the threat just because they perceived it as more threatening, but further research is necessary to confirm this. In conclusion, Lactobacillus supplementation may be a suitable tool to reduce anxiety, promote a more appropriate response to a challenge and so improve welfare.
Metabolomics improves the histopathological diagnosis of asphyxial deaths: an animal proof-of-concept model
Authors: Locci Emanuela , Chighine Alberto , Noto Antonio , Ferino Giulio , Baldi Alfonso , Varvarousis Dimitrios , Xanthos Theodoros , De-Giorgio Fabio , Stocchero Matteo , d’Aloja Ernesto 
Published: May 2021

AbstractThe diagnosis of mechanical asphyxia remains one of the most difficult issues in forensic pathology. Asphyxia ultimately results in cardiac arrest (CA) and, as there are no specific markers, the differential diagnosis of primitive CA and CA secondary to asphyxiation relies on circumstantial details and on the pathologist experience, lacking objective evidence. Histological examination is currently considered the gold standard for CA post-mortem diagnosis. Here we present the comparative results of histopathology versus those previously obtained by 1H nuclear magnetic resonance (NMR) metabolomics in a swine model, originally designed for clinical purposes, exposed to two different CA causes, namely ventricular fibrillation and asphyxia. While heart and brain microscopical analysis could identify the damage induced by CA without providing any additional information on the CA cause, metabolomics allowed the identification of clearly different profiles between the two groups and showed major differences between asphyxiated animals with good and poor outcomes. Minute-by-minute plasma sampling allowed to associate these modifications to the pre-arrest asphyxial phase showing a clear correlation to the cellular effect of mechanical asphyxia reproduced in the experiment. The results suggest that metabolomics provides additional evidence beyond that obtained by histology and immunohistochemistry in the differential diagnosis of CA.
The nucleoplasmic interactions among Lamin A/C-pRB-LAP2α-E2F1 are modulated by dexamethasone
Authors: Ricci Anastasia , Orazi Sara , Biancucci Federica , Magnani Mauro , Menotta Michele 
Published: May 2021

AbstractAtaxia telangiectasia (AT) is a rare genetic neurodegenerative disease. To date, there is no available cure for the illness, but the use of glucocorticoids has been shown to alleviate the neurological symptoms associated with AT. While studying the effects of dexamethasone (dex) in AT fibroblasts, by chance we observed that the nucleoplasmic Lamin A/C was affected by the drug. In addition to the structural roles of A-type lamins, Lamin A/C has been shown to play a role in the regulation of gene expression and cell cycle progression, and alterations in the LMNA gene is cause of human diseases called laminopathies. Dex was found to improve the nucleoplasmic accumulation of soluble Lamin A/C and was capable of managing the large chromatin Lamin A/C scaffolds contained complex, thus regulating epigenetics in treated cells. In addition, dex modified the interactions of Lamin A/C with its direct partners lamin associated polypeptide (LAP) 2a, Retinoblastoma 1 (pRB) and E2F Transcription Factor 1 (E2F1), regulating local gene expression dependent on E2F1. These effects were differentially observed in both AT and wild type (WT) cells. To our knowledge, this is the first reported evidence of the role of dex in Lamin A/C dynamics in AT cells, and may represent a new area of research regarding the effects of glucocorticoids on AT. Moreover, future investigations could also be extended to healthy subjects or to other pathologies such as laminopathies since glucocorticoids may have other important effects in these contexts as well.
Meta-analysis and metaregression of risk factors associated with mortality in hip fracture patients during the COVID-19 pandemic
Authors: Raheman Firas J. , Rojoa Djamila M. , Nayan Parekh Jvalant , Berber Reshid , Ashford Robert 
Published: May 2021

AbstractIncidence of hip fractures has remained unchanged during the pandemic with overlapping vulnerabilities observed in patients with hip fractures and those infected with COVID-19. We aimed to investigate the independent impact of COVID-19 infection on the mortality of these patients. Healthcare databases were systematically searched over 2-weeks from 1st–14th November 2020 to identify eligible studies assessing the impact of COVID-19 on hip fracture patients. Meta-analysis of proportion was performed to obtain pooled values of prevalence, incidence and case fatality rate of hip fracture patients with COVID-19 infection. 30-day mortality, excess mortality and all-cause mortality were analysed using a mixed-effects model. 22 studies reporting 4015 patients were identified out of which 2651 (66%) were assessed during the pandemic. An excess mortality of 10% was seen for hip fractures treated during the pandemic (OR 2.00, p = 0.007), in comparison to the pre-pandemic controls (5%). Estimated mortality of COVID-19 positive hip fracture patients was four-fold (RR 4.59, p < 0.0001) and 30-day mortality was 38.0% (HR 4.73, p < 0.0001). The case fatality rate for COVID-19 positive patients was 34.74%. Between-study heterogeneity for the pooled analysis was minimal (I2 = 0.00) whereas, random effects metaregression identified subgroup heterogeneity for male gender (p < 0.001), diabetes (p = 0.002), dementia (p = 0.001) and extracapsular fractures (p = 0.01) increased risk of mortality in COVID-19 positive patients.