bluuebunny/crossref_metadata_2025_split huggingface dataset:
Silencing of the Long Non-Coding RNA TTN-AS1 Attenuates the Malignant Progression of Osteosarcoma Cells by Regulating the miR-16-1-3p/TFAP4 Axis
Authors: Meng Xianghai , Zhang Zhenjun , Chen Lin , Wang Xi , Zhang Qingguo , Liu Shuheng 
Published: June 2021

ObjectivesOsteosarcoma (OS) is a type of bone malignancy. This study attempted to explore the effect of long non-coding RNA TTN-AS1 (TTN-AS1) on OS and to determine its molecular mechanisms.MethodsThe expression of TTN-AS1, microRNA-16-1-3p (miR-16-1-3p), and transcription factor activating enhancer binding protein 4 (TFAP4) in OS was assessed using qRT-PCR. The OS cell proliferation, migration, and invasion were measured using 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), wound-healing, and transwell assays. N-cadherin and MMP-2 protein level was determined with western blot. Interactions between TTN-AS1 and miR-16-1-3p or TFAP4 and miR-16-1-3p were confirmed using the dual-luciferase reporter assay. Additionally, an OS xenograft tumor model was constructed to assess the effect of TTN-AS1 on tumor growth.ResultsTTN-AS1 and TFAP4 expression was increased in OS, while miR-16-1-3p expression was decreased. TTN-AS1 silencing restrained OS cell proliferation, migration, invasion, N-cadherin and MMP-2 protein expression, and hindered tumor growth. MiR-16-1-3p overexpression retarded the malignant behavior of OS cells. TTN-AS1 played a carcinostatic role by down-regulating miR-16-1-3p in the OS cells. Moreover, miR-16-1-3p inhibition or TFAP4 elevation weakened the suppressive effect of TTN-AS1 silencing on OS cell tumor progression.ConclusionTTN-AS1 promoted the proliferation, migration, invasion, and epithelial-mesenchymal transition (EMT) of OS cells via mediating the miR-16-1-3p/TFAP4 axis. TTN-AS1 may be a critical target for improving OS.
A Machine Learning Model for Predicting a Major Response to Neoadjuvant Chemotherapy in Advanced Gastric Cancer
Authors: Chen Yonghe , Wei Kaikai , Liu Dan , Xiang Jun , Wang Gang , Meng Xiaochun , Peng Junsheng 
Published: June 2021

AimsTo develop and validate a model for predicting major pathological response to neoadjuvant chemotherapy (NAC) in advanced gastric cancer (AGC) based on a machine learning algorithm.MethodA total of 221 patients who underwent NAC and radical gastrectomy between February 2013 and September 2020 were enrolled in this study. A total of 144 patients were assigned to the training cohort for model building, and 77 patients were assigned to the validation cohort. A major pathological response was defined as primary tumor regressing to ypT0 or T1. Radiomic features extracted from venous-phase computed tomography (CT) images were selected by machine learning algorithms to calculate a radscore. Together with other clinical variables selected by univariate analysis, the radscores were included in a binary logistic regression analysis to construct an integrated prediction model. The data obtained for the validation cohort were used to test the predictive accuracy of the model.ResultA total of 27.6% (61/221) patients achieved a major pathological response. Five features of 572 radiomic features were selected to calculate the radscores. The final established model incorporates adenocarcinoma differentiation and radscores. The model showed satisfactory predictive accuracy with a C-index of 0.763 and good fitting between the validation data and the model in the calibration curve.ConclusionA prediction model incorporating adenocarcinoma differentiation and radscores was developed and validated. The model helps stratify patients according to their potential sensitivity to NAC and could serve as an individualized treatment strategy-making tool for AGC patients.
Clinical Characteristics and Survival Outcomes in Neuroblastoma With Bone Metastasis Based on SEER Database Analysis
Authors: He Bin , Mao Jianshui , Huang Leyi 
Published: June 2021

PurposeClinical features and survival analysis of neuroblastoma (NB) are well explored. However, clinical research of NB patients with bone metastasis is rarely reported. Thus, the current study was performed to analyze the clinical features, survival outcome, and risk factors in those patients.Materials and MethodsWe reviewed the Surveillance, Epidemiology, and End Results (SEER) database to select cases diagnosed with NB with bone metastasis from 2010 to 2016. Overall survival (OS) and cancer-specific survival (CSS) were analyzed through univariate Cox regression analysis. Subsequently, we performed multivariate analysis to determine independent predictors of survival. The Kaplan–Meier method was applied to intuitively show differences in prognostic value between independent risk factors.ResultsWe finally identified 393 NB patients with bone metastasis who were selected for survival analysis. Nearly half of the patients (47.3%) were aged &gt;3 years. The adrenal gland was the primary tumor site, accounting for approximately two thirds of cases (66.2%). The 5-year OS and CSS rates of all patients were 62.1% and 64.1%, respectively. The univariate analysis indicated that age, lung metastasis, and tumor size were significantly associated with OS and CSS. Based on the multivariable analysis, age at 2 and 3 years, lung metastasis, and tumor size &gt;10 cm remained significant negative predictors of OS and CSS.ConclusionFor NB patients with bone metastasis, three independent prognostic risk factors (age, lung metastasis, and tumor size) are helpful to clinicians for predicting prognosis and guiding treatment. Reasonable treatment modalities for these patients should be further investigated to prolong survival.
Does Health Literacy Make a Difference? Comparing the Effect of Conventional Medicine Versus Homeopathic Prescribing on Treatment Credibility and Expectancy
Authors: Wilhelm Marcel , Euteneuer Frank 
Published: June 2021

ObjectiveWhile homeopathic remedies are often used to treat non-specific complaints such as headaches, empirical evidence suggests their treatment effect is due to the placebo effect. Low health literacy seems to be connected to higher use of complementary and alternative medicine (CAM). The aim of this study was to examine what people with occasional headaches expect from conventional medicine or homeopathic remedies and if health literacy interacts with this expectation.MethodsIn this experimental study, n = 582 participants with occasional headaches were randomized to read one of two vignettes, which described the prescription of either conventional medicine or a homeopathic remedy. Subsequently, the participants were asked to rate treatment credibility and expectancy with regard to their assigned vignette. Health literacy was assessed as a potential moderator.ResultsParticipants in the conventional medicine group rated treatment credibility and expectancy higher than in the homeopathic remedy group. Moderation analysis revealed that when being offered conventional medicine, participant reports of treatment credibility and expectancy decreased with lower health literacy, while these outcomes increased with lower health literacy for homeopathic remedies.DiscussionPeople with occasional headaches estimate the effectiveness of conventional medication properly. However, health care professionals should pay special attention to patients with low health literacy, as they might need more time and information to give their informed consent.
Microtubule Dynamics Plays a Vital Role in Plant Adaptation and Tolerance to Salt Stress
Authors: Chun Hyun Jin , Baek Dongwon , Jin Byung Jun , Cho Hyun Min , Park Mi Suk , Lee Su Hyeon , Lim Lack Hyeon , Cha Ye Jin , Bae Dong-Won , Kim Sun Tae , Yun Dae-Jin , Kim Min Chul 
Published: June 2021

Although recent studies suggest that the plant cytoskeleton is associated with plant stress responses, such as salt, cold, and drought, the molecular mechanism underlying microtubule function in plant salt stress response remains unclear. We performed a comparative proteomic analysis between control suspension-cultured cells (A0) and salt-adapted cells (A120) established from Arabidopsis root callus to investigate plant adaptation mechanisms to long-term salt stress. We identified 50 differentially expressed proteins (45 up- and 5 down-regulated proteins) in A120 cells compared with A0 cells. Gene ontology enrichment and protein network analyses indicated that differentially expressed proteins in A120 cells were strongly associated with cell structure-associated clusters, including cytoskeleton and cell wall biogenesis. Gene expression analysis revealed that expressions of cytoskeleton-related genes, such as FBA8, TUB3, TUB4, TUB7, TUB9, and ACT7, and a cell wall biogenesis-related gene, CCoAOMT1, were induced in salt-adapted A120 cells. Moreover, the loss-of-function mutant of Arabidopsis TUB9 gene, tub9, showed a hypersensitive phenotype to salt stress. Consistent overexpression of Arabidopsis TUB9 gene in rice transgenic plants enhanced tolerance to salt stress. Our results suggest that microtubules play crucial roles in plant adaptation and tolerance to salt stress. The modulation of microtubule-related gene expression can be an effective strategy for developing salt-tolerant crops.
The Effect of Doping in Split-Well Direct-Phonon THz Quantum-Cascade Laser Structures
Authors: Lander Gower Nathalie , Piperno Silvia , Albo Asaf 
Published: June 2021

We have studied the effect of doping on the temperature performance of a split-well (SW) direct-phonon (DP) terahertz (THz) quantum-cascade laser (QCL) scheme supporting a clean three-level system. Achieving a system that is as close as possible to a clean n-level system proved to be the strategy that led to the best temperature performance in THz-QCLs. We expected to obtain a similar improvement to that observed in resonant-phonon (RP) schemes after increasing the carrier concentration from 3 × 1010 cm−2 to 6 × 1010 cm−2. Our goal was to improve the temperature performance by increasing the doping, ideally the results should have improved. To our surprise, in the devices we checked, the results show the contrary. Although an increase in doping had previously shown a positive effect in RP schemes, our results indicated that this does not happen with SW–DP devices. However, we observed a significant increase in gain broadening and a reduction in the dephasing time as the doping and temperature increased. We attribute these effects to enhanced ionized-impurity scattering (IIS). The observation and study of effects related to dephasing included in our experimental work have previously only been possible via simulation.
Effect of different sources and doses of sulphur on yield, nutrient content and uptake by spring wheat
Authors: Juhász Evelin Kármen , Balláné Kovács Andrea 
Published: June 2021

The objective of this study was to investigate the effect of two sulphur forms (sulphate and tiosulphate) in combination with three different N:S ratios on the yield of spring wheat and total N- and S-content and uptake by the aboveground biomass on chernozem and sandy soil. In the greenhouse experiment, the effects of two sulphur forms were compared: sulphate (SO42-) and thiosulphate (S2O32-). The sulphate was applied as potassium-sulphate (K2SO4) and thiosulphate as ammonium-thiosulphate ((NH4)2S2O3). Increasing doses of both sulphur forms (24, 60, 120 kg S ha-1) were used with the same nitrogen dose (120 kg N ha-1) which caused three different N:S ratios background (1:0.2, 1:0.5, 1:1). Nitrogen was supplied in the form of monoammonium-phosphate (MAP), ammonium-nitrate and ammonium-thiosulphate. Plant samples were taken in three different development stages of spring wheat based on the BBCH scale: at the stage of BBCH 30–32 (stem elongation), BBCH 65–69 (flowering) and BBCH 89 (ripening). The total nitrogen and total sulphur content of plant at different development stages and also wheat grain were measured by Elementar Vario EL type CNS analyser. The nutrient uptake by plant and grain was calculated from the yield of spring wheat and the N and S content of plant. The grain yield on chernozem soil ranged between 6.31 and 12.13 g/pot. All fertilised treatments significantly increased the grain yield compared to the control. The highest yield was obtained in the case of the application of 120 kg N ha-1 and 60 kg S ha-1in sulphate form. The grain yield on sandy soil varied from 2.53 to 6.62 g/pot. The fertilised treatments significantly enhanced the yield compared to the control. The highest yield was observed in the case of the application of 120 kg N ha-1 and 60 kg S ha-1 in thiosulphate form. On chernozem soil the increasing doses of sulphur (24, 60, 120 kg S ha-1) with the same N dose (120 kg N ha-1) increased the N-content of spring wheat at all development stages and in the grain. The treatments with different sulphur sources did not cause further changes in the N-content. On sandy soil in the most cases the N-content did not change significantly as a result of increasing sulphur doses. The treatments with sulphate form basically resulted higher nitrogen-content than treatments with thiosulphate form. The treatments with increasing sulphur doses resulted higher S-content on both of chernozem and sandy soil in the case of all development stage. Comparing the effect of the applied sulphur sources on the S-content it can be stated that at the stage of BBCH 30–31 and 65–69 the treatments with sulphate form resulted higher sulphur-content. At the stage of BBCH 89 there was no significant differences in S-content of grain as a result of different sulphur-sources.
Avaliação de segurança de medicamento off-label utilizados no tratamento da COVID-19: revisão sistemática/ Safety assessment of off-label medication used in the treatment of COVID-19: Systematic review
Authors: Oliveira Fabianny Silva de , Mendonça Gleyze da Silva , Silva Socorro de Souza 
Published: June 2021

INTRODUÇÃO: O SARS-CoV-2 é um vírus de ácido ribonucleico, composto de uma fita de RNA positiva, com genoma formado por uma molécula de ribose, um ácido fosfórico e uma base nitrogenada, destacando-se a proteína spike e nucleoproteína que compõem a glicoproteína de pico. A glicoproteína faz com que o SARS-CoV-2 penetre na célula do hospedeiro pela ligação do receptor e membrana, fazendo a replicação do vírus em seu interior, ocasionando a transmissão do vírus.OBJETIVO: Analisar as principais reações adversas dos medicamentos utilizados de forma off-label no tratamento da COVID -19.MÉTODOS: Utilizou-se as palavras chaves nos bancos de dados Scielo (Scientific Electronic Library Online), Pubmed (Biblioteca Nacional de Medicina) e Lilacs (Literatura Latina – Americana e do Caribe em ciência da saúde. RESULTADOS: Ao final da pesquisa, foram encontrados nos bancos de dados 120 artigos, desses, 8 na plataforma Scielo, 39 no Lilacs e 73 no Pubmed. Após as etapas de seleção, foram excluídos 109 artigos por meio dos critérios de exclusão e selecionados para análise 11 artigos completos.CONCLUSÕES: Os estudos apontam para a falta de evidências conclusivas aos benefícios e a segurança das intervenções terapêuticas contribuindo para a morbidade e mortalidade de pacientes com COVID-19. A utilização de medicamentos off- label manifestou uma quantidade bem significativa de reações adversas, superando os benefícios clínicos.
Regionalización de la Cirugía Oncológica Torácica en Chile
Authors: Perez Pablo , Santolaya Raimundo , Berrios Raul , Ortega Jose , Zink Manfred 
Published: June 2021

La patología oncológica representa la segunda causa de muerte en Chile. Si bien se han realizado importantes avances como las garantías explicitas de salud para 17 patologías malignas, la Ley Ricarte Soto para enfermedades de alto costo y la Ley del Cáncer Dr. Claudio Mora, aun existen importantes diferencias de sobrevida basados en estrato socioeconómico y desgraciadamente no existen datos nacionales sobre diferencias en resultados clínicos entre centros de alto y bajo volumen. Para avanzar en un cuidado de pacientes con cáncer que permita a la población de Chile recibir una buena atención y optar a los mejores resultados oncológicos posibles, se hace necesario concentrar la experiencia en cáncer en un grupo mas reducido de centros regionales que puedan concentrar recursos y experiencia.
Ambient-hydrogen-induced changes in the characteristics of Pt/GaN Schottky diodes fabricated on bulk GaN substrates
Authors: Irokawa Yoshihiro , Ohki Tomoko , Nabatame Toshihide , Koide Yasuo 
Published: June 2021

Abstract
Hydrogen-induced changes in the characteristics of Pt/GaN rectifiers fabricated on bulk GaN were studied using current–voltage (I–V), capacitance–voltage (C–V), and impedance spectroscopy measurements. The results were similar to those for Pt/GaN rectifiers fabricated on sapphire substrates [Y. Irokawa, Jpn. J. Appl. Phys. 59, 120901 (2020)]. That is, an ambient H2 atmosphere reduced the Schottky barrier height and the resistance of the semiconductor space-charge region but did not affect the ideality factor, carrier concentration, or capacitance of the semiconductor space-charge region, suggesting that the quality of the GaN layers was not the origin of the observed H2-induced changes.
Hipertensión Arterial de pacientes con covid-19 en el Hospital General Manta
Authors: López Parrales José Xavier , Espinoza Lucas Milton , Castelo Caiza Michael Javier 
Published: June 2021

La enfermedad COVID-19 es el tercer brote epidémico de neumonía por coronavirus, causado por coronavirus SARS-CoV-2, en lo que va de este siglo. Afecta de manera más severa a grupos vulnerables, como los adultos mayores, con inmunodepresión y enfermedades crónicas entre ellas, la hipertensión arterial, de gran relevancia en la mortalidad por enfermedades cardiovasculares y cerebrovasculares, siendo un marcador clínico de gravedad de pacientes con COVID-19, manifestado fundamentalmente en edades mayores de 60 años, y la suspensión de forma preventiva de los mencionados tratamientos antihipertensivos puede conducir a complicaciones clínicas de corto a largo plazo, El objetivo del estudio fue determinar la prevalencia de la hipertensión arterial en pacientes diagnosticados con Covid-19 en el Hospital IESS-Manta, Ecuador. Se realizó un estudio de enfoque cuantitativo, observacional descriptivo de corte transversal, retrospectivo, en pacientes comprendidos entre los 20 hasta 65 años,que ingresaron desde Marzo hasta Octubre del 2020 al Hospital de segundo nivel y de referencia para pacientes conCOVID-19 en la Provincia de Manabí-Ecuador. Con una población de 905 sujetos diagnosticados con Covid-19 se analizaron las variables controladas de edad y sexo. El análisis estadístico se basó en la estadística descriptiva de la base de datos AS400 de la historia Clínica del Centro de Salud. La prevalencia de pacientes hipertensos diagnosticados con Covid-19 es de 58 por cada 100 pacientes que ingresaron por covid-19 y edad comprendida entre 20 a 65 equivale al 84.8 %.
Diseño de un manual de procesos y procedimientos para la Clínica Veterinaria de Pequeñas Especies
Authors: Arteaga Macías Johanna , Arteaga Majojo Laura , Navarrete Suarez Gustavo , Mendoza Saltos María , Palma Macías Gregorio 
Published: June 2021

Las clínicas veterinarias deben aspirar a lograr un buen desempeño en los servicios que brindan, para lograrlo deben enfocarse en fortalecer los procesos y procedimientos que van a permitir dar un mejor seguimiento en todas las áreas incluyendo la docencia. El objetivo del estudio fue diseñar un manual de procesos y procedimientos para cada uno de los lineamientos de la clínica veterinaria de pequeñas especies de la Universidad Técnica de Manabí, Ecuador. La investigación fue de tipo descriptiva, utilizando los métodos deductivos, cognitivos. Se aplicaron encuestas, entrevistas, y cuestionario de control interno. La muestra se realizó por conveniencia, con 50 usuarios y 15 estudiantes pasantes. Los principales resultados destacan que los pasantes entienden las funciones principales que se desarrollan de manera cotidiana en la clínica. El 87% de los sujetos encuestados manifiestan estar totalmente insatisfechos y el 13% lo consideran parcialmente satisfactorio, esto evidencia la necesidad de contar con un manual de procesos y procedimientos. Respecto a la rapidez del servicio de veterinaria con relación a los pacientes que llegan a la clínica, el 75% de los sujetos refiere estar totalmente de acuerdo, mientras que el 19% está de acuerdo y solamente el 5,6% dice estar indeciso. Se concluye que el manual de procesos y procedimientos utilizados permite asegurar la calidad de los servicios veterinarios que ofrece la clínica de pequeñas especies, la herramienta cuenta con los elementos básicos para fortalecer el centro y se logra una mejor actuación de los estudiantes pasantes en las actividades de la clínica.
Metodología del aprendizaje basado en problemas como una herramienta para el logro del proceso de enseñanza- aprendizaje
Authors: Velazquez Raquel Vera , Maldonado Zúñiga Kirenia , Castro Piguave Carlos , Batista Garcet Yoiler 
Published: June 2021

El aprendizaje basado en problemas es una metodología que tiene como punto de partida la adquisición e integración de los nuevos conocimientos, donde los estudiantes son los protagonistas de su aprendizaje, en la que los docentes facilitan este proceso. La investigación fue desarrollada en la Universidad Estatal del Sur de Manabí en la carrera de Agropecuaria. El objetivo del estudio fue evaluar el uso de la metodología del aprendizaje basado en problemas como una herramienta para el logro del proceso de enseñanza- aprendizaje en los estudiantes, utilizando la experiencia de docentes de diferentes enseñanzas y el estudio de artículos e investigaciones realizadas sobre el tema. Se utilizaron métodos teóricos (análisis y síntesis), empíricos (técnica observación y encuestas) y métodos estadísticos para determinar la muestra y tabular los resultados del instrumento aplicado, la muestra estuvo conformada por 9 docentes de diferentes especialidades, Los principales resultados destacan que la evaluación de la metodología el aprendizaje basado en problemas como herramienta para el logro del aprendizaje en los estudiantes, demostraron que es de gran importancia, para desarrollar la motivación, el aprendizaje significativo, el pensamiento crítico y creativo, la integración de conocimientos, la comprensión, el desarrollo de habilidades, trabajo colaborativo y participativo.
Caracterización clínico- epidemiológica del paciente con hematuria en el servicio de clínicas pediátricas. 2020.
Authors: Sánchez Pérez Yusleidy Yanet , Elías Montes Yelenis , Tamayo Cordoví Alitza , Morales Torres Glenis , Linares Rodríguez Annia María 
Published: June 2021

La hematuria es un signo frecuente en pediatría y un reto diagnóstico en muchos casos. El objetivo del estudio fue caracterizar la hematuria en pacientes ingresados en el Hospital Provincial Pediátrico Docente ¨General Milanés ¨ durante el año 2020. Se realizó un estudio, descriptivo, en niños ingresados con Hematuria en el Hospital Provincial Pediátrico Docente ¨General Milanés¨, desde enero – diciembre de 2020. El universo estuvo constituido por 86 pacientes, ingresados con hematuria macroscópica en el servicio de clínicas pediátricas. Se estudiaron las siguientes variables: edad, sexo, tipos de hematuria, síntomas y signos acompañantes, antecedentes familiares y personales y el diagnóstico definitivo al egreso. Los principales resultados destacan que el grupo de edad entre 5 y 9 años (46.02%) y el sexo masculino con (79.06 %). La disuria predomino en 45 pacientes (52.32%) y los antecedentes patológicos personales, predominaron los niños sin antecedentes para un 59.30%. En los antecedentes patológicos familiares, la hipertensión arterial predominó en el 54.55% de los niños y el 47.68% presentaron Hematuria no glomerular inespecífica, 41 pacientes. Se concluye que la hematuria predominó en el grupo etario de 5 a 9 años y el sexo masculino. La disuria fue el síntoma más frecuente así como los niños sin antecedentes patológicos personales, la hipertensión arterial constituyó el antecedente patológico familiar que predominó en la serie y el diagnóstico definitivo al egreso que más se presentó fue la hematuria no glomerular.