bluuebunny/crossref_metadata_2025_split huggingface dataset:
Platelet Priming Following Naturally Occurring Thermal Burn Injuries And Wildfire Smoke Exposure is Associated with Intracardiac Thrombosis in Feline Survivors
Authors: Li Ronald H. L. , Tan Avalene W. K. , Ueda Yu , Stern Joshua A. , Hussain Mehrab , Haginoya Satoshi , Sharpe Ashley N. , Gunther-Harrington Catherine T. , Epstein Steven E. , Nguyen Nghi 
Published: August 2021

Abstract
Wildfires pose a major health risk for humans, wildlife, and domestic animals. We previously discovered pathophysiologic parallels between domestic cats with naturally occurring smoke inhalation and thermal burn injuries and human beings with similar injuries; these were characterized by transient myocardial thickening, cardiac troponin I elevation and formation of intracardiac thrombosis. While the underlying mechanisms remain unclear, results from murine models suggest that platelet priming and activation may contribute to a global hypercoagulable state and thrombosis. Herein, we evaluated and compared the degree of platelet activation, platelet response to physiologic agonists and levels of platelet-derived microvesicles (PDMV) in 29 cats with naturally occurring wildfire thermal injuries, 21 clinically healthy cats with subclinical hypertrophic cardiomyopathy (HCM) and 11 healthy cats without HCM (CC). We also quantified and compared circulating PDMVs in WF cats to CC cats. In addition, we examined the association between thrombotic events, severity of burn injuries, myocardial changes, and the degree of platelet activation in cats exposed to wildfires. Flow cytometric detection of platelet surface P-selectin expression showed that cats in the wildfire group (WF) had increased platelet response to adenosine diphosphate (ADP) and thrombin compared to the 2 control groups indicating the presence of primed platelets in circulation. In addition, cats in the WF group had increased circulating levels of PDMV, characterized by increased phosphatidylserine on the external leaflet. Cats in the WF group with documented intracardiac thrombosis had elevated platelet activation and platelet priming in the presence of ADP or thrombin. While high dose arachidonic acid (AA) mostly resulted in platelet inhibition, persistent platelet activation in response to AA was noted among cats in the WF group with intracardiac thrombosis. Univariate and multiple logistic regression analyses demonstrated that increased platelet response to AA was independently associated with thrombotic events. This is the first study reporting the significant association between platelet priming and intracardiac thrombosis in domestic cats with naturally occurring wildfire-related injuries and smoke inhalation. Further studies are required to delineate additional mechanisms between inflammation and thrombosis, especially regarding platelet primers and the cyclooxygenase pathway.
Analysis Of Dose At 3D-Crt And Imrt Techniques In Left Breast Cancer Case With Hypofractionation Using Deep Inspiration Breath-Hold (Dibh)
Authors: - Samsun - , Arif Muhammad Arif , Septayudha Gregorius Septayudha 
Published: August 2021

Abstract
In terms of breast cancer radiation treatment, it has radiation using the 3D-Conformal Radiotherapy (3D-CRT) technique and the continuation of the 3D-CRT technique, namely the Intensity Modulated Radiation Therapy (IMRT) technique. This study aims to evaluate the dosage aspects of PTV and OAR between the 3D-CRT and IMRT techniques in cases of left breast cancer with hypofractionation using the Deep Inspiration Breath Hold (DIBH) method using the Conformity Index (CI) and Homogeneity Index (H.I.) and H.I. organ at risk uses tolerance limits. This type of research is comparative quantitative with ten samples with primary data conducted at Siloam T.B. Hospital. Simatupang from November 2019 to April 2020. The research was carried out in the form of radiation planning with 3D-CRT techniques and IMRT techniques, and the results of planning both techniques were evaluated between 3D-CRT techniques and IMRT techniques through PTV evaluations using CI and H.I. values. Furthermore, the organs at risk use tolerance limits on each organ. The results showed the assessment between 3D-CRT and IMRT on PTV and organs at risk received different doses. The PTV shows the CI value, which is almost the same as the difference of 0.034, and there is a slight difference in H.I. with an average value in the IMRT technique of 0.07 and 3D-CRT of 0.11, and it can be seen that the IMRT is slightly superior because the excellent H.I. value is the closest to 0. Then at the dose of organ at risk received by the sample, the 3D-CRT technique is slightly superior by obtaining a lower dose that obtains the difference in the heart by 0.53%, lung by 3.46%, spinal cord by 6.51 Gy, esophagus at 4.5 Gy, and larynx at 5.18 Gy.
Design and Synthesis of a Siderophore-Based Fluorescent Probe and Its Application in Selective Detection of Aeromonas
Authors: Cisneros-Sureda Javier , Rey-Varela Diego , Rodríguez Jaime , Balado Miguel , Lemos Manuel L. , Jiménez Carlos 
Published: August 2021

Abstract
Amonabactins, the siderophores produced by some pathogenic bacteria belonging to Aeromonas genus, can be used for the preparation of conjugates that can be imported into the cell using their specific transport machinery. Herein, we report the design and synthesis of a new amonabactin-based fluorescent probe by conjugation of the appropiate amonabactin analogue to sulforhodamine B (AMB-SRB) using a thiol-maleimide click reaction. Growth promotion assays and fluorescence microscopy studies demonstrated that AMB-SRB fluorescent probe was able to label the fish pathogenic bacterium A. salmonicida subsp. salmonicida through its outer membrane transport (OMT) protein FstC. The labelling of other Aeromonas species such as the human pathogenic A. hydrophila, indicates that this probe can be a very useful molecular tool for studying the amonabactin-dependent iron uptake mechanism. Furthermore, the selective labelling of A. salmonicida and other Aeromonas species in presence of other fish pathogenic bacteria, suggest the potential application of this probe for detection of Aeromonas in water and other fish farming samples through fluorescence assays.
SARS-CoV-2 Delta Variant Predominant at a Tertiary-Care Hospital in Saudi Arabia
Authors: Alhamlan Fatimah , Al-Qahtani Ahmed , Obeid Dalia , Aljumaah Suliman , Alghamdi Salem , ALNAFEE KHALED , Alsanea Madain , Alahideb Basma , Almutairi Shuruq , Alsuwairi Feda , Al-Abdulkarim Maha , Asiri Mohamed , Alkahtani Jalwa , Altamimi Sara , Mutabagani Maysoon , Althawadi Sahar , Almaghrabi Reem 
Published: August 2021

Abstract
Despite the effectiveness of the Pfizer-BioNTech and Oxford-AstraZeneca vaccines against SARS-CoV-2, the delta (B.1.617.2) variant of concern (VOC) has spread globally, and breakthrough infections have been reported. We identified the circulating variants and effectiveness of those vaccines between April and June 2021 at a tertiary-care hospital in Saudi Arabia. Among 320 patients with confirmed COVID-19 (mean age, 39 years; 53.6% male), 70.6% received a one-dose vaccination; 15.1% received two-dose vaccinations; 14. 3% were unvaccinated; 64.9% received Oxford-AstraZeneca; and 32.5% received Pfizer-BioNTech. Most breakthrough cases involving VOCs (71%) occurred among patients who received a one-dose Oxford-AstraZeneca vaccination, and most patients with breakthrough disease were infected with the delta variant. Among all VOCs and non-VOCS, the delta variant was most frequently detected and was associated with young age (20–49 years), males, symptoms, and low cycle threshold value. These findings indicate an increased rate of transmission for the delta variant in this cohort.
The neuromorphic Mosaic: re-configurable in-memory small-world graphs
Authors: Dalgaty Thomas , Moro Filippo , De Pra Alessio , Indiveri Giacomo , Vianello Elisa , Payvand Melika 
Published: August 2021

Abstract
Thanks to their non-volatile and multi-bit properties, memristors have been extensively used as synaptic weight elements in neuromorphic architectures. However, their use to define and re-program the network connectivity has been overlooked.
Here, we propose, implement and experimentally demonstrate Mosaic, a neuromorphic architecture based on a systolic array of memristor crossbars. For the first time, we use distributed non-volatile memristors not only for computation, but also for routing (i.e., to define the network connectivity). Mosaic is particularly well-suited for the implementation of re-configurable small-world graphical models, with dense local and sparse global connectivity - found extensively in the brain. We mathematically show that, as the networks scale up, the Mosaic requires less memory than in conventional memristor approaches. We map a spiking recurrent neural network on the Mosaic to solve an Electrocardiogram (ECG) anomaly detection task. While the performance is either equivalent or better than software models, the advantage of the Mosaic was clearly seen in respective one and two orders of magnitude reduction in energy requirements, compared to a micro-controller and address-event representation-based processor. Mosaic promises to open up a new approach to designing neuromorphic hardware based on graph-theoretic principles with less memory and energy.
Seroprevalence of Antibodies against SARS COV- 2 among Health Care Workers at Tertiary Care Hospital, Uttarakhand: A Retrospective study
Authors: Saini Amit K , Panda Prasan Kumar , Bahurupi Yogesh Arvind , Omar Balram , T Akhil , Panwar Pooja , Sharma Maneesh 
Published: August 2021

Abstract
Background: The number of confirmed SARS-CoV-2 infections is vastly underestimated. In this context, Seroprevalence surveys are of utmost importance to assess the proportion of the population that has already developed antibodies against the virus and might potentially be protected against subsequent infection. Health care workers face greater risk of developing SARS-CoV-2. Therefore, present retrospective study was undertaken to estimate the prevalence of antibodies against SARS-CoV-2 among healthcare workers at tertiary care institute, Uttarakhand. Material and Methods : Data was gathered from Hospital records of 704 healthcare workers admitted at COVID 19 Unit and attended Covid-OPD of tertiary care institute, Uttarakhand in-between 15th July to 14th Aug 2020. Result: Out of the 704 recruited participants, 14 (1.99%) were seropositive for IgG antibodies against SARS-CoV-2. The cumulative prevalence of SARS-CoV-2 infection (presence of antibodies or past or current positive RT-PCR) was 4.40%. Conclusion: The present study shows a low prevalence of SARS-CoV-2 IgG antibodies among health care workers. In addition, posting in COVID-19-positive areas was not associated with increased seropositivity. More studies are warranted to assess IgG/IgM antibodies against SARS-CoV-2 among those HCW who are exposed to COVID-19 patients.
Four-dimensional trajectory optimization model with priority in continuous time period
Authors: Li Meng , Chu Jianyu , Li Yinfeng , Wu Xianjie 
Published: August 2021

This article aims to mitigate the imbalance of capacity and flow in airspace. Targeting to minimize the total delay time, taking the real-life sector operation and aircraft flight rules into consideration, with constraints of sector capacity limit and minimal time interval, the principle to prioritize aircraft when the delay occurred was raised. A trajectory planning model with the priority of aircraft was then established in the model of trajectory based on operation in a continuous period. The traditional genetic algorithm was also improved through the strategy of enhanced elitism preserving and double-stranded chromosome structure. Case studies indicated that the proposed trajectory planning model and solution algorithm have contributed to, over the two periods, an average reduction of 71.77 % in the delay time on optimization effect, and an increase of 19.48 % in the calculation speed. In this case, the model appears to, in a relatively short time, provide a trajectory allocation strategy with security and timeliness, for aircraft operated in consecutive periods. As a result, the sectors can operate without any conflicts while effectively reducing flight delays, minimizing the traffic congestion and potential accidents, so as to take the most advantages of sector resources allocation.
Seismic performance analysis of self-centering segment piers with mortise-tenon shear connectors based on cyclic pseudo-static test
Authors: Ni Yongjun , Hao Changshun , Xu Yanzhou 
Published: August 2021

In the past decades, more and more precast piers have been used in actual engineering, which brings convenience in construction and standardization of production. However, precast piers still have some shortcomings, such as shear slip between segments and poor energy dissipation. Thus, a self-centering mortise-tenon segmental bridge pier was proposed in this paper. To research the seismic performance of the self-centering mortise-tenon segment piers, three scaled model piers with their scale ratio as 1:3 were designed and prefabricated, namely as a cast-in-place model pier (CP) and 2 self-centering mortise-tenon segment model piers (designated as MTSP1 and MTSP2) with the initial pretension of 1302 MPa and 1488 MPa, respectively. The cyclic pseudo-static tests of the three piers were carried out. The comparison analysis was made respecting to the test results including the damage form, hysteretic characteristics, skeleton curve, energy dissipation capacity, residual displacement and equivalent stiffness of the three specimens. It was shown that MTSP specimens had higher horizontal bearing capacity. While the yield load, ultimate load, displacement ductility coefficient and initial stiffness of MTSP1 and MTSP2 were higher than those of CP. The MTSP had better performance considering the above aspects. The mean value of the residual displacement of MTSP2 was lower than that of CP 21.14 % and 29. 72 % respectively, as the drift ratio reaching 5 %. The MTSP specimens had better self-centering capacity due to the increased pretension stress which reducing the residual displacement greatly. The energy dissipation reinforcements improved the energy dissipation capacity of the segmental assembled piers. Based on the ABAQUS model, the numerical simulation was carried out and compared with the experimental data. MTSP had great energy dissipation capacity and self-centering capacity. It was suggested that MTSP should be used to make up for the deficiency of segmental assembled pier and improve the seismic performance of segmental assembled pier.
Characterization of bacterial fluorescence: insight into rapid detection of bacteria in water
Authors: Mao Yu , Chen Xiao-Wen , Chen Zhuo , Chen Gen-Qiang , Lu Yun , Wu Yin-Hu , Hu Hong-Ying 
Published: August 2021

Abstract
Microbial contamination is one of the main risks affecting water safety. Traditional microbial detection methods tend to be time-consuming and labor-intensive. Thus, this study investigated a potential rapid and simple method for bacterial detection in water by excitation–emission matrix (EEM) fluorescence spectroscopy. Particularly, bacterial intrinsic fluorophores were divided into three regions, namely Region A (amino acids), Region N (NAD(P)H) and Region F (flavins). Afterwards, fluorescence characteristics of four pure bacterial species (Bacillus subtilis, Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa) as well as indigenous bacteria in secondary effluent from two water reclamation plants were evaluated via fluorescence regional integration (FRI). Correlation analysis between fluorescence intensity (FI) integral and bacterial concentration was conducted, and principal component analysis (PCA) was applied to distinguish the fluorescence spectra of different bacteria. The results showed that most of the bacterial autofluorescence was emitted by amino acids and the FI integral of flavins had a good linear relationship (R2 &gt; 0.9) with bacterial concentration. PCA could distinguish varied bacterial species and bacteria from different secondary effluents. This study indicated that FRI was helpful for the characterization of bacterial fluorescence and the quantification of bacteria in water.
Numerical investigation of flow field around T-shaped spur dyke in a reverse-meandering channel
Authors: Tripathi Ravi Prakash , Pandey K. K. 
Published: August 2021

Abstract
In this paper, the flow characteristics around T-shape spur dyke situated in the reverse meandering channel with a rigid bed is simulated using renormalization group (RNG) turbulence model with ANSYS 2018 Fluent software. To solve the model in 3D ways we used Navier-Stroke's equation based on the principle of conservation of mass and momentum within a moving fluid. For studying the flow characteristics, computational fluid dynamics were applied with all geometric parameters and the turbulence was simulated using (RNG) equations of model. In this simulation, the structured meshes were used with different diameters and diameters of meshes are high at the exit channel for obtaining accuracy in the result. In this study, we mainly focus on the effect of Froude number on flow pattern and several other characteristics like velocity distribution, flow separation, bed-shear-stress distribution. The final result of this research work is compared with the condition when no structure is present in the channel.
Generation and Characterization of Monoclonal Antibodies Against Tth DNA Polymerase and its Application to Hot-Start PCR
Authors: Tang Yuting , Chen Xiaoyu , Zhang Jian , Wang Jin , Hu Wenhao , Liu Songbai , Luo Zhidan , Xu Henghao 
Published: August 2021

Background:
As a heat-resistant polymerase, Thermus thermophilus (Tth) DNA polymerase
can be widely used in Polymerase Chain Reaction (PCR). However, its non-specific amplification
phenomenon is serious, which greatly limits development.

Objective:
In this study, we prepared Tth monoclonal antibodies against Tth DNA polymerase and
researched their application in hot-start PCR.

Methods:
Tth was recombinantly expressed and purified, and used as an antigen to immunize BALB/
c mice to obtain monoclonal antibodies. The qualified monoclonal antibody and Tth were incubated
for a period of time at a certain temperature to obtain the hot-start Tth. We tested the polymerase
activity and exonuclease activity blocking the performance of hot-start Tth. Finally, the
hot-start Tth was applied to one-step RT-PCR.

Results:
Tth with a purity of >95% was obtained, and ten monoclonal antibodies were obtained by
immunization. After incubation, three monoclonal antibodies were identified that could inhibit the
polymerase activity of Tth at low temperature. Furthermore, these three antibodies successfully
eliminated non-specific amplification in practical applications.

Conclusion:
Three monoclonal antibodies were successfully validated. Among them, monoclonal
antibody 9 had the best overall effect. They possess the function of inhibiting at low temperature
and releasing at high temperature, which can be used as Tth polymerase inhibitors in the field of
molecular diagnostics.
New Scope of Targeted Therapies in Lung Carcinoma
Authors: Sonkar Archana , Kumar Pranesh , Gautam Anurag , Maity Biswanath , Saha Sudipta 
Published: August 2021

:
Lung Cancer (LC) is the leading cause of cancer deaths worldwide. Recent research has
also shown LC as a genomic disease, causing somatic mutations in the patients. Tests related to mutational
analysis and genome profiles have lately expanded significantly in the genetics/genomics field
of LC. This review summarizes the current knowledge about different signalling pathways of LC
based on the clinical impact of molecular targets. It describes the main molecular pathways and
changes involved in the development, progression, and cellular breakdown of LC and molecular
changes. This review focuses on approved and targeted experimental therapies such as immunotherapy
and clinical trials that examine the different targeted approaches to treating LC. We aim to clarify
the differences in the extent of various genetic mutations in DNA for LC patients. Targeted molecular
therapies for LC can be continued with advanced racial differences in genetic changes, which have a
significant impact on the choice of drug treatment and our understanding of the profile of drug susceptibility/
resistance. The most relevant genes described in this review are EGFR, KRAS, MET, BRAF,
PIK3CA, STK11, ERBB3, PTEN, and RB1. Combined research efforts in this field are required to
understand the genetic difference in LC outcomes in the future.
Bone Deleterious Effects of Different NRTIs in Treatment-naïve HIV Patients After 12 and 48 Weeks of Treatment
Authors: Atencio Patricia , Conesa-Buendía Francisco Miguel , Cabello-Ubeda Alfonso , Llamas-Granda Patricia , Pérez-Tanoira Ramón , Prieto-Pérez Laura , Álvarez Beatriz Álvarez , Acosta Irene Carrillo , Arboiro-Pinel Rosa , Díaz-Curiel Manuel , Largo Raquel , Herrero-Beaumont Gabriel , Górgolas Miguel , Mediero Aránzazu 
Published: August 2021

Background:
Bone alterations have been observed in the course of HIV infection, characterized
by a marked decrease in bone mineral density (BMD) and an increase in the frequency of
fractures as a result of fragility. We aim to evaluate early changes in bone metabolic profile and the
possible association with tenofovir and other nucleoside and nucleotide reverse transcriptase inhibitors
(NRTIs) in treatment-naïve HIV patients.

Methods:
We conducted a prospective study in naïve HIV-infected adults (under 50 years), separated
into three groups according to NRTI therapy: tenofovir disoproxil fumarate (TDF); tenofovir
alafenamide (TAF) and abacavir (ABC). BMD and epidemiological, immunological and metabolic
bone parameters were evaluated. Bone markers were analyzed in plasma at baseline, 12 and 48
weeks after initiating treatment.

Results:
Average age of patients was 34.8 years (± 9.6). 92.4% of them with CD4 count > 200
cel/μL. At week 12 after starting treatment, both TDF [increase in PN1P (31.7%, p = 0.004), TRAP
(11.1%, p = 0.003), OPN (19.3%, p = 0.045) and OC (38.6%, p = 0.001); decrease in OPG
(-23.4%, p = 0.003)] and TAF [increase in 42.6% for CTX (p = 0.011), 27.3% for OC (p = 0.001)
and 21% for TRAP (p = 0.008); decrease in OPG (-28.8%, p = 0.049)] presented a deep resorption
profile compared to ABC, these differences in bone molecular markers, a tendency to equalize at
week 48, where no significant differences were observed. Patients treated with TDF showed the
greatest decrease in Z-score in both lumbar spine (LS) and femoral neck (FN) at week 48 without
statistically significant differences.

Conclusions:
Treatment-naïve HIV patients have a high prevalence of low bone density. Treatment
with TDF is associated with greater bone deterioration at 12 and 48 weeks. TAF seems to present
similar early bone deterioration at 12 weeks which disappears at 48 weeks.