bluuebunny/crossref_metadata_2025_split huggingface dataset:
Correlation between semen examination based on the WHO criteria and sperm DNA fragmentation test
Authors: Okubo Tsuyoshi , Onda Noriyuki , Hayashi Teruaki , Omi Kenji , Segawa Tomoya 
Published: May 2021

Abstract
In this study, we performed and analyzed the DFI test and semen test based on the WHO criteria and compared the two using semen factors. DFI tests and general semen tests were conducted in accordance with the WHO criteria, and correlations between the two tests were investigated. The WHO criteria were set as the cutoff value for each of the following factors: fluid volume, concentration, motility, and normal morphology, and compared with the DFI results. The subjects had a mean DFI of 15.6 ± 11.5% and the DFI increased with age. In contrast, the concentration, motility, and normal morphology decreased as the DFI increased. Patients who satisfied the WHO criteria in terms of sperm concentration, motility, and normal morphology had a significantly lower DFI than the group that did not satisfy the criteria. Therefore, evaluation with a general semen test based on the WHO criteria should be regarded as a qualitative evaluation of all factors other than fluid volume. Male infertility due to sperm DNA fragmentation should be suspected when in vitro fertilization (IVF) results are poor despite normal semen findings based on the WHO criteria. The results of this study suggest that the DFI test may be an important evaluation in follow-up semen analyses.
Data Optimization in IoT-Assisted Sensor Networks on Cloud Platform
Authors: Suseendran G. , Akila D. , Pal Souvik , Sarkar Bikramjit , Aly Ayman A. , Le Dac-Nhuong 
Published: May 2021

Abstract
This article presents a new scheme for data optimization in IoT assister sensor networks. The various components of IoT assisted cloud platform are discussed. In addition, a new architecture for IoT assisted sensor networks is presented. Further, a model for data optimization in IoT assisted sensor networks is proposed. A novel Membership inducing Dynamic Data Optimization (MIDDO) algorithm for IoT assisted sensor network is proposed in this research. The proposed algorithm considers every node data and utilized membership function for the optimized data allocation. The proposed framework is compared with two stage optimization, dynamic stochastic optimization and sparsity inducing optimization and evaluated in terms of performance ratio, reliability ratio, coverage ratio and sensing error. It was inferred that the proposed MIDDO algorithm achieves an average performance ratio of 76.55%, reliability ratio of 94.74%, coverage ratio of 85.75% and sensing error of 0.154.
Sjögren's Syndrome in Children: About 15 Cases in Guinea Conakry
Authors: Condé Kaba , Guelngar Carlos Othon , Barry Mamadou Ciré , Atakla Hugues Ghislain , Mohamed Awada , Cissé Fodé 
Published: May 2021

Abstract
Objectives- Sjögren's syndrome is difficult to diagnose in the African context. It is rarely reported in children in Black Africa. We report a series of 15 cases of Sjögren’s syndrome in order to clarify the particularities of this condition in children.Methods- This 2-year retrospective study focused on children under 16 years of age with a male predominance who were followed for AS in the rheumatology and pediatrics departments. Patient data were collected and analyzed using STATA/SE version 11.2 software. Anonymity and respect for ethical rules were the norm. There was no link between patients and researchers.Results- The average age of the patients was 11 years with extremes of 5 to 15 years. An anamnesis revealing dry mouth was found in more than half of the cases, i.e. in 10 (66.7%) patients. The clinical examination found oral ulceration and periodontitis in equal proportions, i.e. 6 (40%). The immunological assessment and the biopsy of the accessory salivary glands were used as diagnostic evidence in the 15 patients according to the American-European criteria of 2002.Conclusion- Sjögren's syndrome is a rare entity in pediatrics. It is difficult to diagnose in pediatrics and its severity is linked to the occurrence of visceral and lymphomatous late dry syndrome. Rapid diagnosis and the use of a synthetic antimalarial drug (Hydroxychloroquine) increases the hope of a cure.
Volumetric comparative analysis of anatomy through far-lateral approach: surgical space and exposed tissues
Authors: Tang Ke , Xu Feng , Yuan Xiaodong , Li Yang , Chen Xinyue 
Published: May 2021

Abstract
Background
The three-dimensional (3D) visualization model has ability to quantify the surgical anatomy of far-lateral approach. This study was designed to disclose the relationship between surgical space and exposed tissues in the far-lateral approach by the volumetric analysis of 3D model.
Methods
The 3D skull base models were constructed using MRI and CT data of 15 patients (30 sides) with trigeminal neuralgia. Surgical corridors of the far-lateral approach was simulated by triangular pyramids to represent two surgical spaces exposing bony and neurovascular tissues. Volumetric comparison of surgical anatomy was performed using pair t test.
Results
The morphometric results were almost the same in the two surgical spaces except the vagus nerve (CN X) exposed only in one corridor. Whereas, the volumetric comparison represented the statistical significant differences of surgical space, and bony and neurovascular tissues involved in the two corridors (P༜0.001). The differences of bony and neurovascular tissuesfailed to equal the difference of surgical space.
Conclusions
For far-laterl approach, the increase of exposure for the bony and neurovascular tissues is not necessarilymatched with the increase of surgical space. The volumetric comparative analysis is helpful to provide more detailed anatomical information in the surgical design.
Olanzapine 5mg vs 10mg for the Prophylaxis of Chemotherapy-Induced Nausea and Vomiting – A Network Meta-Analysis
Authors: Chow Ronald , Navari Rudolph M , Terry Bryan , DeAngelis Carlo , Prsic Elizabeth 
Published: May 2021

Abstract
Introduction: Olanzapine administered at a 10mg dosage for prophylaxis of chemotherapy-induced nausea and vomiting may be associated with fatigue, drowsiness and reduced general activity. Therefore, a 5mg dose may be preferred, to reduce the occurrence of adverse events. The aim of this study was to conduct a network meta-analysis, and report on the efficacy of olanzapine administered at 5mg, relative to when administered at 10 mg. Methods: We used previously-published data from the systematic review by Chow et al which identified 17 adult trials which used 10mg doses, 3 which used 5mg doses, and 1 which used a mix of 5 and 10mg doses. A multivariate network meta-analysis using a restricted maximum likelihood model was used. Results: The complete response rate in the acute phase is not statistically different, between 5mg and 10mg doses of olanzapine – RR 0.97, 95% CI: 0.83 – 1.13. Additionally, in the overall phase, 5mg olanzapine is similarly as efficacious as 10mg olanzapine – RR 0.95, 95% CI: 0.56 – 1.60. Evaluation of data demonstrated that there was inadequate information to compare the toxicities of the two doses. Conclusion: Our analyses support individual published trials, and the rationale for future trials to compare 5mg to 10mg olanzapine regimens in head-to-head comparisons.
Exogenous melatonin regulates chromium stress induced feedback inhibition of photosynthesis and antioxidative protection in Brassica napus cultivars
Authors: Ayyaz Ahsan , Farooq Ahsan , Zafar Zafar Ullah , Rahman Habib ur 
Published: May 2021

Abstract
Developing heavy metal chromium (Cr) tolerant cultivars is mainly constrained due to poor knowledge of the mechanism behind Cr stress tolerance. In the present study, available germplasm of Brassica napus (canola) was screened out for Cr stress tolerance using Dual-PAM-100 and biochemical attributes and used to selected one Cr stress tolerant (Ac-Excel) and one sensitive (DGL) under melatonin (MT) treatments for further studies. Plant oxidative enzymes and PSII activity were found to be key physiological discriminating factors. Changes in PSII and PSI efficiency, oxidative damage, higher proline content and antioxidants were further evaluated in selected Cr tolerant and sensitive cultivars. Cr stress reduced PSII efficacy and ETC in both canola cultivars. MT application increased NPQ and Y(NPQ), higher increase in NPQ and Y(NPQ) were observed in Cr stress tolerant cv AC-Excel, indicating that the MT treated tolerant cultivar have higher excitation of PSII by more heat dissipation via photo-protective component of NPQ under Cr stress. Reduced PSI efficiency and increased donor end limitation of PSI in both canola cultivars further confirmed the downregulation of electron transport from PSII. However, Cr stress sensitive cv DGL had poor efficacy to manage over-excitation of PSII via buildup of Y(NPQ) through increased oxidative stress. Tolerant cultivar exhibited higher proline content and antioxidant enzymes activity than Cr sensitive cultivar. Overall, MT induced tolerance in canola cultivars can be related to PSII activity, Y(NPQ), and antioxidants potential which can be effectively used for canola cultivars selection under Cr stress.
Bladder malignant granular cell tumor with EP300 gene mutation: a case report and literature review
Authors: Huang Jian , Zhu Di , Ren Xinle , Huang Bing 
Published: May 2021

Abstract
Background Malignant granular cell tumor (GCT) is extremely rare. Malignant GCT with EP300 gene mutation in the bladder has not been reported in the literature. Case presentation We report a special case of 45 years old female with malignant GCT of the bladder. Physical examination found a pelvic mass in the patient. Magnetic resonance imaging showed a huge mass between the posterior wall of the bladder, the cervix, and the anterior wall of the vagina. Pathological examination showed that the mass was 11×11×4.5cm in size, involved in the bladder's posterior wall. Under the microscope, the tumor cells were arranged in the shape of a nest or cord to infiltrate the bladder's wall. The tumor cells were pleomorphic, red-stained granular within the cytoplasm, with increased nuclear/cytoplasmic ratio, vacuolar nuclei, and obvious nucleoli. The tumor cells were showed obvious nuclear atypia, and the mitosis was more than 5/50HPF. Coagulative necrosis was widely showed within the tumor. Immunohistochemistry(IHC) showed that S-100, NSE, CD68, CR, α-AT, and TFE-3 were strongly positive, and the Ki-67 proliferation index was around 15%. The next-generation high throughput sequencing indicated that EP300 gene was missense mutated (c.457A>G) with 33% mutation abundance, and genes of DPYD(c.1627A>G),ERCC1( c.354T>C),NQO1(c.559C>T),TPMT(c.719A>G) and XRCC1(c.1196A>G) were polymorphic mutated. The patient died after three months of the second surgical treatment. Conclusions We report for the first time a primary bladder malignant GCM accompanied by mutations in special driving genes such as EP300. We also conducted a comprehensive literature review and an in-depth discussion.
Lymphoblastic lymphoma in two young siblings (coincidence or genetics?): a case report
Authors: Hassan Naya Talal , Makhoul Ebrahim , Sallameh Jaafar , Ghanem Abdulmunem , Rajab Samer , Ali Waseem , Alshehabi Zuheir 
Published: May 2021

Abstract
Background: Non-Hodgkin lymphoma is the fourth most common malignancy in children and it is not considered to be a hereditary disorder. However, it could affect members from the same family.Case presentation: We are presenting two cases of Caucasian female siblings who were diagnosed with mediastinal lymphoblastic lymphoma in the same year. The two young females were presented to the emergency department with respiratory symptoms. After doing radiological investigations and biopsies, the diagnosis of lymphoblastic lymphoma was done. The elder sister died before confirming the diagnosis, and the other one is on chemotherapy now and the treatment outcomes is good.Conclusions: This case emphasize the crucial role of precursor genetics in lymphoblastic lymphomas and suggesting the strong relation between those genetics and the age of symptoms presentation. This is the first report of non-Hodgkin siblings pair in the pediatric population.
In vitro organogenesis and plant regeneration of Passiflora xishuangbannaensis, a species with extremely small populations
Authors: Meng Yuan-yuan , Song Shi-jie , Landrein Sven 
Published: May 2021

Abstract
Passiflora xishuangbannaensis (Passifloraceae) is endemic to a few sites of Mengyang nature reserve in Yunnan, Xishuangbanna and less than 40 individuals have been recorded. Nine Passiflora species are endemic to Yunnan with most species occurring in South America, making P. xishuangbannaensis highly significant and emblematic to the conservation work in the region. This study is designed to provide the first protocol for in vitro organogenesis and plant regeneration for ex situ conservation and reintroduction for an Asian Passiflora species. Using internodes, petioles and tendrils we optimize calli formation and root elongation using several plant growth regulators, individually or in combination. We also assess the genetic stability of regenerated cells. The maximum callus induction and shoot bud differentiation were both achieved on half Murashige and Skoog basal medium supplemented with 4.44 µM 6-Benzylaminopurine and 1.08 µM 1-Naphthaleneacetic acid. The best rooting was achieved from 30 days old, regenerated shoots on half Murashige and Skoog basal medium supplemented with 1.08 µM 1-Naphthaleneacetic acid. Micropropagated plants were subjected to inter simple sequence repeat markers analyses. Collectively, 86 bands were generated from 6 primers of which 12 bands were polymorphic, showing genetic variation between the regenerated plantlets and the original plant. Response to plant growth regulators was more specific than most other studies using South American species, which could be explained by the morphological and physiological differences between South American and Asian Passiflora species
A new point mutation in SIX1 causes Branchio-oto syndrome, thymoma and pure red cell aplasia via mitochondrial apoptosis
Authors: Sun Runjie , Pisano Michael , Gao Yi , Sun Fumou , Shi Jin , Xu Jie , Dong Xueyan , Zheng Wei , Zhang Yanyu , Sun Xiaoqi , Cui Xing 
Published: May 2021

Abstract
A patient was admitted to the hospital with thymoma, branchio-oto syndrome and pure red aplastic anemia. He had both Six1 and mitochondrial mutations. The Six1 gene has been linked to branchio-oto-renal spectrum disorder and malignant tumors. Additionally, mitochondrial mutations are known to cause anemia as well. To this end, we designed experiments to explore the relationship between Six1 mutations, mitochondrial function and these diseases. Whole-exome sequencing (WES) was used for gene detection, and transfected 293T cells were used for in vitro experiments. CCK-8 and flow cytometry were used to detect cell proliferation, cell cycle stage and apoptotic populations. Caspase-3 activity was used as an apoptosis indicator. The effects on mitochondria were examined by ROS and JC-1. Western blotting was used to detect the expression of Bcl-2, Bax and cleaved caspase-3. WES revealed a Six1c.374A > G point mutation in the proband and deaf members of his family, as well as, a mitochondrial mutation (ND3) in the proband. 293T cells transfected with Six1 were used as the WT group, the Six1c.374A > G transfection group was used as the MUT group, and the untransfected 293T cells were used as the NC group. A CCK-8 test found that the cell proliferation rates were reduced both in the WT group and the MUT group, and MUT group cells were found to be blocked in S-phase. Due to these findings, apoptosis was investigated. Following investigation, increased levels of ROS, caspase-3 activity and cleaved caspase-3 were detected in WT and MUT groups. Following these results, mitochondrial function was measured. The level of mitochondrial membrane potential in WT and MUT groups was decreased significantly. Western blot showed that and the Bcl-2/Bax ratio decreased. Six1c.374A > G point mutation can affect cell proliferation through increased apoptosis controlled by the mitochondrial apoptotic pathway. This could be the cause of the patient's pure red cell aplasia.
The Perils of a “My Work Here is Done” Perspective: A Mixed Methods Evaluation of Sustainment of an Intervention to Improve Quality of Transient Ischemic Attack Care
Authors: Bravata Dawn M , Miech Edward J , Myers Laura J , Perkins Anthony J , Zhang Ying , Rattray Nicholas A , Baird Sean A , Penney Lauren S , Austin Charles , Damush Teresa M 
Published: May 2021

Abstract
BACKGROUND Sustaining quality improvement has been recognized as a key implementation challenge. The objectives of this study were to evaluate sustainment during the implementation of a novel quality improvement intervention for Transient Ischemic Attack (TIA) across six geographically diverse medical centers and to identify specific factors contributing to sustainment. METHODS The PREVENT study was a five-year, stepped-wedge implementation trial at six US Department of Veterans Affairs sites; active implementation was initiated in three waves, with two facilities per wave. Six control sites were matched to each of the six PREVENT active implementation sites (total number of control sites was 36). Mixed methods were used to assess change in quality of care from baseline to active implementation to sustainment (measured by the “without-fail rate” and to identify factors which promoted or hindered sustainment. RESULTS The without-fail rate at PREVENT sites improved from 36.7% at baseline to 54.0% during active implementation and settled to 48.3% during sustainment. At control sites, the without-fail rate improved from 38.6% at baseline to 41.8% during active implementation and remained steady at 43.0% during sustainment. Changes in quality of care during sustainment varied across PREVENT sites: the without-fail rate improved at three sites, declined at two sites, and remained unchanged at one site. In adjusted analyses, although the without-fail rate improved at PREVENT sites compared with control sites during active implementation, no statistically significant difference in quality between intervention and control sites was identified during the sustainment phase. Factors that promoted sustainment were integration of key processes of care into routine practice and establishing systems for reflecting and evaluating on performance data to plan quality improvement activities or respond to changes in quality. Challenges during sustainment included competing demands from new facility quality priorities, low patient volume, and potential problems with coding impairing use of performance data. CONCLUSIONS Facilities seeking to sustain evidence-based practices while embodying the Learning Healthcare System’s core values can harness the combined power of staff and data systems by embedding quality improvement processes within routine care and establishing systems for reviewing and reflecting upon performance data.TRIAL REGISTRATION clinicaltrials.gov: NCT02769338
Automatic diagnosis and biopsy classification with dynamic Full-Field OCT and machine learning
Authors: Thouvenin Olivier , Scholler Jules , Mandache Diana , Mathieu Marie Christine , Lakhdar Aïcha Ben , Darche Marie , Monfort Tual , Boccara Claude , Olivo-Marin Jean-Christophe , Grieve Kate , Yedid Vannary Meas , Guillaume Emilie Benoit a la 
Published: May 2021

Abstract
The adoption of emerging imaging technologies in the medical community is often hampered if they provide a new unfamiliar contrast that requires experience to be interpreted. Here, in order to facilitate such integration, we developed two complementary machine learning approaches, respectively based on feature engineering and on convolutional neural networks (CNN), to perform automatic diagnosis of breast biopsies using dynamic full field optical coherence tomography (D-FF-OCT) microscopy. This new technique provides fast, high resolution images of biopsies with a contrast similar to H&E histology, but without any tissue preparation and alteration. We conducted a pilot study on 51 breast biopsies, and more than 1,000 individual images, and performed standard histology to obtain each biopsy diagnosis. Using our automatic diagnosis algorithms, we obtained an accuracy above 88% at the image level, and above 96% at the biopsy level. Finally, we proposed different strategies to narrow down the spatial scale of the automatic segmentation in order to be able to draw the tumor margins by drawing attention maps with the CNN approach, or by performing high resolution precise annotation of the datasets. Altogether, these results demonstrate the high potential of D-FF-OCT coupled to machine learning to provide a rapid, automatic, and accurate histopathology diagnosis.
Geographic distance and vegetation structure rather than river barrier drive ant beta diversity across Central Amazonia
Authors: Guilherme Diego Rodrigues , Pequeno Pedro Aurélio Costa Lima , Baccaro Fabrício Beggiato , Franklin Elizabeth , Neto Cláudio Rabelo dos Santos , de Souza Jorge Luiz Pereira 
Published: May 2021

Abstract
To understand better the effects of niche and neutral processes is important to disentangle the direct and indirect effects of each process, mainly if the environmental factors are geographically structured neglecting important indirect and synergic effects. We sampled ground-dwelling ant species on 126 plots distributed across eight sampling sites along a broad environmental gradient in Central Amazonia. Structural equation modelling was employed to quantify direct and indirect effects of geographic distance, the Amazon River’s opposite margins, and environmental differences in temperature, precipitation and vegetation structure (Normalized Difference Vegetation Index) on ant beta diversity (Jaccard’s dissimilarity). We found that geographic distance and NDVI differences had major direct effects on ant beta diversity. The major effect of temperature was indirect through NDVI, whereas precipitation had no detectable effect on beta diversity. The Amazon River had a weak influence on the ant composition dissimilarity. Our results challenge the major role often ascribed to riverine barriers in the diversification and distribution of Amazonian biota. Rather, ant compositional dissimilarity seems to be mainly driven by a combination of dispersal limitation and selection imposed by vegetation features and, indirectly, by temperature. We suggest that as NDVI differences decrease with geographic distance in the region, isolation by distance may have favoured phenotypic convergence between ant communities in the northern and southern borders of the Amazon Basin.