bluuebunny/crossref_metadata_2025_split huggingface dataset:
Transcutaneous penetration of a single-chain variable fragment (scFv) compared to a full-size antibody: potential tool for atopic dermatitis (AD) treatment
Authors: Baylet Audrey , Vyumvuhore Raoul , Laclaverie Marine , Marchand Laëtitia , Mainzer Carine , Bordes Sylvie , Closs-Gonthier Brigitte , Delpy Laurent 
Published: July 2021

AbstractCurrently, several biologics are used for the treatment of cutaneous pathologies such as atopic dermatitis (AD), psoriasis or skin cancers. The main administration routes are subcutaneous and intravenous injections. However, little is known about antibody penetration through the skin. The aim was to study the transcutaneous penetration of a reduced-size antibody as a single-chain variable fragment (scFv) compared to a whole antibody (Ab) and to determine its capacity to neutralize an inflammatory cytokine involved in AD such as human interleukin-4 (hIL-4). Transcutaneous penetration was evaluated by ex vivo studies on tape-stripped pig ear skin. ScFv and Ab visualization through the skin was measured by Raman microspectroscopy. In addition, hIL-4 neutralization was studied in vitro using HEK-Blue™ IL-4/IL-13 cells and normal human keratinocytes (NHKs). After 24 h of application, analysis by Raman microspectroscopy showed that scFv penetrated into the upper dermis while Ab remained on the stratum corneum. In addition, the anti-hIL4 scFv showed very efficient and dose-dependent hIL-4 neutralization. Thus, scFv penetrates through to the upper papillary dermis while Ab mostly remains on the surface, the anti-hIL4 scFv also neutralizes its target effectively suggesting its potential use as topical therapy for AD.
SARS-CoV-2: from its discovery to genome structure, transcription, and replication
Authors: Brant Ayslan Castro , Tian Wei , Majerciak Vladimir , Yang Wei , Zheng Zhi-Ming 
Published: July 2021

AbstractSARS-CoV-2 is an extremely contagious respiratory virus causing adult atypical pneumonia COVID-19 with severe acute respiratory syndrome (SARS). SARS-CoV-2 has a single-stranded, positive-sense RNA (+RNA) genome of ~ 29.9 kb and exhibits significant genetic shift from different isolates. After entering the susceptible cells expressing both ACE2 and TMPRSS2, the SARS-CoV-2 genome directly functions as an mRNA to translate two polyproteins from the ORF1a and ORF1b region, which are cleaved by two viral proteases into sixteen non-structural proteins (nsp1-16) to initiate viral genome replication and transcription. The SARS-CoV-2 genome also encodes four structural (S, E, M and N) and up to six accessory (3a, 6, 7a, 7b, 8, and 9b) proteins, but their translation requires newly synthesized individual subgenomic RNAs (sgRNA) in the infected cells. Synthesis of the full-length viral genomic RNA (gRNA) and sgRNAs are conducted inside double-membrane vesicles (DMVs) by the viral replication and transcription complex (RTC), which comprises nsp7, nsp8, nsp9, nsp12, nsp13 and a short RNA primer. To produce sgRNAs, RTC starts RNA synthesis from the highly structured gRNA 3' end and switches template at various transcription regulatory sequence (TRSB) sites along the gRNA body probably mediated by a long-distance RNA–RNA interaction. The TRS motif in the gRNA 5' leader (TRSL) is responsible for the RNA–RNA interaction with the TRSB upstream of each ORF and skipping of the viral genome in between them to produce individual sgRNAs. Abundance of individual sgRNAs and viral gRNA synthesized in the infected cells depend on the location and read-through efficiency of each TRSB. Although more studies are needed, the unprecedented COVID-19 pandemic has taught the world a painful lesson that is to invest and proactively prepare future emergence of other types of coronaviruses and any other possible biological horrors.
Hierarchical coordinated anti-jamming channel access in clustering networks: a multi-leader multi-follower Stackelberg game approach
Authors: Xu Yifan , Chen Jin , Feng Zhibin , Yao Kailing , Li Guoxin , Song Fei , Fang Gui 
Published: July 2021

AbstractThis paper mainly investigates the multi-user coordinated anti-jamming problem in clustering communication networks. In such kinds of networks, there exist multiple clusters and multiple users who communicate with their receivers simultaneously. Besides, a malicious jammer persistently attacks channels with wide-band and dynamic changing jamming signals. To cope with these challenges brought by the large-scale clustering network and the dynamic wide-band jamming, a hierarchical coordinated anti-jamming approach is proposed, and a multi-leader multi-follower Stackelberg game is introduced to model the anti-jamming problem. In detail, cluster heads act as leaders, and select available frequency bands to avoid jamming attacks, while users in each cluster act as followers and select corresponding channels distributedly and independently. Moreover, it is proved that there exist multiple Stackelberg equilibriums (SEs) in the proposed game. To obtain SEs, a hierarchical coordinated anti-jamming channel access (HCACA) algorithm is designed. Simulation results illustrate that the proposed approach is effective to cope with the dynamic wide-band jamming attacks. Furthermore, it is also depicted that the proposed approach outperforms the distributed anti-jamming comparative approach in terms of convergence speed.
Safety and efficacy of different antibiotic regimens in patients with ocular toxoplasmosis: systematic review and meta-analysis
Authors: Feliciano-Alfonso John E. , Muñoz-Ortiz Juliana , Marín-Noriega María Alejandra , Vargas-Villanueva Andrés , Triviño-Blanco Laura , Carvajal-Saiz Natalia , de-la-Torre Alejandra 
Published: July 2021

AbstractBackgroundOcular toxoplasmosis (OT) is the most common cause of posterior uveitis, which leads to visual impairment in a large proportion of patients. Antibiotics and corticosteroids lower the risk of permanent visual loss by controlling infection and inflammation. However, there remains disagreement regarding optimal antibiotic therapy for OT. Therefore, this systematic review and meta-analysis were performed to determine the effects and safety of existing antibiotic treatment regimens for OT.MethodsMEDLINE, EMBASE, The Cochrane Central Register of Controlled Trials, LILACS, WHO International Clinical Trials Registry Platform portal, ClinicalTrials.gov, and Gray Literature in Europe (“OpenGrey”) were searched for relevant studies; manual searches of reference lists were performed for studies identified by other methods. All published and unpublished randomized controlled trials that compared antibiotic schemes known to be effective in OT at any dosage, duration, and administration route were included. Studies comparing antibiotics with placebo were excluded. This review followed standard methodological procedures recommended by the Cochrane group.ResultsTen studies were included in the narrative summary, of which four were included for quantitative synthesis (meta-analysis). Interventions were organized into three groups: intravitreal clindamycin versus pyrimethamine + sulfadiazine, trimethoprim + sulfamethoxazole versus other antibiotics, and other interventions. The first comparison favored intravitreal clindamycin (Mean difference (MD) = 0.10 logMAR; 95% confidence interval = 0.01 to 0.22). However, this finding lacks clinical relevance. Other outcomes showed no statistically significant differences between the treatment groups. In general, the risk of performance bias was high in evaluated studies, and the quality of the evidence found was low to very low.ConclusionsNo antibiotic scheme was superior to others, and the selection of a treatment regimen depends on multiple factors; therefore, treatment should be chosen based on safety, sulfa allergies, and availability.
Geotechnical evaluation of gully erosion and landslides materials and their impact in Iguosa and its environs, southern Nigeria
Authors: Aigbadon Godwin Okumagbe , Ocheli Azuka , Akudo Ernest Orji 
Published: July 2021

Abstract
Background
Detailed field surveys and geotechnical evaluation of soils in Iguosa and its environs, Southern Nigeria, were undertaken to determine the root causes, mechanisms, and impacts of landslides and gully erosion. This was done to suggest appropriate mitigation measures to reclaim the affected land and prevent future occurrences in the study area.

Results
Field study revealed high elevations, a steep slope, high rainfall and inadequate drainage systems. Also, human activities and socio-cultural activities have contributed to the large lateral extents in depths and widths of the landslides and gullies in the study area. The geotechnical analyses reveal that soil samples from SB1, SB2, SB6 and SB7 lithological units are mainly sandy clay with a coefficient of permeability ranging from 3.5 × 10−4 to 4.2 × 10−4 cm/s, the cohesion ranges from 27 to 28 kƿa and angle of internal resistance ranges from 27° to 30° respectively. The plastic limit ranges from 2 to 4, and liquid limit ranges from 33 to 38, and the plasticity index ranges from 30 to 36. Ajali sand units SB3, SB4, SB8 and SB9, consist of coarse-grained sand with no plasticity. The coefficient of permeability ranges from 2.8 × 10−4 to 3.2 × 10−4 cm/s, the cohesion range from 10 to 18 kƿa, angle of internal resistance 24° to 26°, respectively. The soil samples from SB5 and SB10 lithological units are silty-clay with a coefficient of permeability of 4.6 × 10−4 to 4.8 × 10−4 cm/s. The cohesion of 45 to 46 kƿa, and angle of internal resistance of 37﻿° to 40°, respectively. The plastic limits ranges from 35 to 36, and liquid limit is 76, and the plasticity index ranges from 40 to 41.

Conclusion
Field survey and geotechnical evaluations of the soils revealed that high elevation, a steep slope and the geotechnical properties of the soils were the initial conditions that initiated landslides and gully developments in the study area. This has also been influenced by rainfall, poor vegetation, inadequate drainage systems, and human activities as well as socio-cultural activities. Over four hundred and thirty-two houses and farmlands and other properties have been damaged and abandoned in the study area. Covering the landslide areas with impermeable layers/materials, diverting surface water away from the landslide areas, enacting laws to prevent the erection of structures on landslide prone-areas, sound drainage systems, the use of biotechnical slope and bioengineering methods, afforestation and re-vegetation were the proposed mitigation measures to tackle this menace in the study area.
ACER3-related leukoencephalopathy: expanding the clinical and imaging findings spectrum due to novel variants
Authors: Dehnavi Ali Zare , Heidari Erfan , Rasulinezhad Maryam , Heidari Morteza , Ashrafi Mahmoud Reza , Hosseini Mohammad Mahdi , Sadeghzadeh Fatemeh , Fallah Mohammad-Sadegh , Rostampour Noushin , Bahraini Amir , Garshasbi Masoud , Tavasoli Ali Reza 
Published: July 2021

Abstract
Background
Leukodystrophies are the main subgroup of inherited CNS white matter disorders which cause significant mortality and morbidity in early years of life. Diagnosis is mostly based on clinical context and neuroimaging findings; however, genetic tools, particularly whole-exome sequencing (WES), have led to comprehending the causative gene and molecular events contributing to these disorders. Mutation in Alkaline Ceramidase 3 (ACER3) gene which encodes alkaline ceramidase enzyme that plays a crucial role in cellular growth and viability has been stated as an uncommon reason for inherited leukoencephalopathies. Merely only two ACER3 mutations in cases of progressive leukodystrophies have been reported thus far.

Results
In the current study, we have identified three novel variants in ACER3 gene in cases with new neurological manifestations including developmental regression, dystonia, and spasticity. The detected variants were segregated into family members.

Conclusion
Our study expands the clinical, neuroimaging, electroencephalographic, and genetic spectrum of ACER3 mutations. Furthermore, we reviewed and compared the findings of all the previously reported cases and the cases identified here in order to facilitate their diagnosis and management.
Text Data Augmentation for Deep Learning
Authors: Shorten Connor , Khoshgoftaar Taghi M. , Furht Borko 
Published: July 2021

AbstractNatural Language Processing (NLP) is one of the most captivating applications of Deep Learning. In this survey, we consider how the Data Augmentation training strategy can aid in its development. We begin with the major motifs of Data Augmentation summarized into strengthening local decision boundaries, brute force training, causality and counterfactual examples, and the distinction between meaning and form. We follow these motifs with a concrete list of augmentation frameworks that have been developed for text data. Deep Learning generally struggles with the measurement of generalization and characterization of overfitting. We highlight studies that cover how augmentations can construct test sets for generalization. NLP is at an early stage in applying Data Augmentation compared to Computer Vision. We highlight the key differences and promising ideas that have yet to be tested in NLP. For the sake of practical implementation, we describe tools that facilitate Data Augmentation such as the use of consistency regularization, controllers, and offline and online augmentation pipelines, to preview a few. Finally, we discuss interesting topics around Data Augmentation in NLP such as task-specific augmentations, the use of prior knowledge in self-supervised learning versus Data Augmentation, intersections with transfer and multi-task learning, and ideas for AI-GAs (AI-Generating Algorithms). We hope this paper inspires further research interest in Text Data Augmentation.
The risks of warm nights and wet days in the context of climate change: assessing road safety outcomes in Boston, USA and Santo Domingo, Dominican Republic
Authors: Nazif-Munoz José Ignacio , Martínez Pablo , Williams Augusta , Spengler John 
Published: July 2021

Abstract
Background
There remains a dearth of cross-city comparisons on the impact of climate change through extreme temperature and precipitation events on road safety. We examined trends in traffic fatalities, injuries and property damage associated with high temperatures and heavy rains in Boston (USA) and Santo Domingo (Dominican Republic).

Methods
Official publicly available data on daily traffic outcomes and weather conditions during the warm season (May to September) were used for Boston (2002–2015) and Santo Domingo (2013–2017). Daily maximum temperatures and mean precipitations for each city were considered for classifying hot days, warm days, and warm nights, and wet, very wet, and extremely wet days. Time-series analyses were used to assess the relationship between temperature and precipitation and daily traffic outcomes, using a quasi-Poisson regression.

Results
In Santo Domingo, the presence of a warm night increased traffic fatalities with a rate ratio (RR) of 1.31 (95% CI [confidence interval]: 1.00,1.71). In Boston, precipitation factors (particularly, extremely wet days) were associated with increments in traffic injuries (RR 1.25, 95% CI: 1.18, 1.32) and property damages (RR 1.42, 95% CI: 1.33, 1.51).

Conclusion
During the warm season, mixed associations between weather conditions and traffic outcomes were found across Santo Domingo and Boston. In Boston, increases in heavy precipitation events were associated with higher traffic injuries and property damage. As climate change-related heavy precipitation events are projected to increase in the USA, the associations found in this study should be of interest for road safety planning in a rapidly changing environment.
A comparative geological, tectonic and geomorphological assessment of the Charlotte, Regent and Madina landslides, Western area, Sierra Leone
Authors: Lahai Yusuf Alhaji , Anderson Kelvin F. E. , Jalloh Yaguba , Rogers Ibrahim , Kamara Mohamed 
Published: July 2021

AbstractThis work focused on three landslide events that have attracted significant public concern due to the associated calamities they recorded in 1945, 2017 and 2019, i.e. the Charlotte, Regent and Madina landslides, respectively. Their geology, tectonics (structural discontinuities) and geomorphology, i.e. their GTG characteristics were studied to establish links between them and the landslide events.Field surveys were conducted, particularly on the Charlotte landslide, where the identification of geological structures was impeded to an extent by its obliteration by vegetation and sediment accumulations on relatively planar sections of the landslide area. Remote sensing and GIS techniques (earth imagery and drone images) enhanced the mapping and determination of landslides’ geometric and geomorphic parameters. Laboratory analyses of rock and soil samples provided the landslides’ petrological characterisation and were used to determine the particle-size distribution in the slide-prone soil.The study indicated a change in the gabbroic rock composition, variable geomorphological characteristics, and nature/pattern and density of the discontinuities. These factors, to a large extent, determined the nature and magnitude of the rainfall-triggered landslides. Charlotte lithology slightly differed from the other two landslides and recorded higher Silica (Si) and Aluminum (Al) and lower iron (Fe) from X-Ray Fluorescence (XRF) than rocks of Regent and Madina landslides. The study also revealed only a tenuous correlation between rock composition and weathering depth. The slope angles at the landslides’ prominent scarps (depletion zone) are steep (> 45 degrees) with altitudes of approximately 270 m, 200 m and 470 m above sea level for Charlotte, Regent and Madina, respectively. Unlike the Charlotte landslide, both Regent and Madina landslides are active, but geometrically, their area, length and run-out distances have relatively high variance with a coefficient of variance equals to 1. Information derived from this work can help understand the spatial variation in landslide characteristics and develop a susceptibility map.
Retention of different temporary cements tested on zirconia crowns and titanium abutments in vitro
Authors: Dähne Felix , Meißner Heike , Böning Klaus , Arnold Christin , Gutwald Ralf , Prause Elisabeth 
Published: July 2021

Abstract
Purpose
The aim of the present study was to examine the retention force of monolithic zirconia copings cemented with various temporary cements on implant abutments in vitro.

Methods
Sixty exercise implants with pre-screwed implant abutments were embedded in resin. Subsequently, 60 CAD/CAM manufactured zirconia copings were divided into three main groups [Harvard Implant Semi-permanent (HAV), implantlink semi Forte (IMP), Temp Bond NE (TBNE)]. The zirconia copings were cemented on the implant abutments and loaded with 35 N. Specimens were stored in distilled water (37 °C) for 24 h. Half of the test specimens of each group were subjected to a thermocycling (TC) process. Retention force was measured in a universal testing machine. Using magnifying glasses, the fracture mode was determined. Statistical analysis was performed applying the Kruskal-Wallis test, the post hoc test according to Dunn-Bonferroni and a chi-square test of independence.

Results
Without TC, IMP showed the highest retention of the three temporary luting agents (100.5 ± 39.14 N). The measured retention forces of IMP were higher than those of HAV (45.78 ± 15.66 N) and TBNE (61.16 ± 20.19 N). After TC, retention was reduced. IMP showed the greatest retentive strength (21.69 ± 13.61 N, three fail outs). HAV and TBNE showed pull-off forces of similar magnitude (17.38 ± 12.77 N and 16.97 ± 12.36 N, two fail outs). The fracture mode analysis showed different results regarding the tested cements before and after TC (facture type before/after TC): IMP (III+II/III), HAV (I/II) and TBNE (III/III). There were clear differences of the fracture modes regarding the examination before and after TC.

Conclusions
Within the limits of this study, IMP showed the highest pull-off forces under the chosen test conditions. All three temporary luting agents showed lower retention forces after TC. Retention values in the individual cement classes were very heterogeneous. Easy cement removal in the crown lumen favours the dominance of adhesive cement fractures on the abutment and adhesive/cohesive cement fractures on the abutment with HAV appears advantageous in case of recementation of the superstructure.
High-Intensity Interval Training in Older Adults: a Scoping Review
Authors: Marriott Catherine F. S. , Petrella Andrea F. M. , Marriott Emily C. S. , Boa Sorte Silva Narlon C. , Petrella Robert J. 
Published: July 2021

AbstractHigh-intensity interval training (HIIT) is an increasingly popular form of aerobic exercise which includes bouts of high-intensity exercise interspersed with periods of rest. The health benefits, risks, and optimal design of HIIT are still unclear. Further, most research on HIIT has been done in young and middle-aged adults, and as such, the tolerability and effects in senior populations are less well-known. The purpose of this scoping review was to characterize HIIT research that has been done in older adults including protocols, feasibility, and safety and to identify gaps in the current knowledge. Five databases were searched with variations of the terms, “high-intensity interval training” and “older adults” for experimental or quasi-experimental studies published in or after 2009. Studies were included if they had a treatment group with a mean age of 65 years or older who did HIIT, exclusively. Of 4644 papers identified, 69 met the inclusion criteria. The average duration of training was 7.9 (7.0) weeks (mean [SD]) and protocols ranged widely. The average sample size was 47.0 (65.2) subjects (mean [SD]). Healthy populations were the most studied group (n = 30), followed by subjects with cardiovascular (n = 12) or cardiac disease (n = 9), metabolic dysfunction (n = 8), and others (n = 10). The most common primary outcomes included changes in cardiorespiratory fitness (such as VO2peak) as well as feasibility and safety of the protocols as measured by the number of participant dropouts, adverse events, and compliance rate. HIIT protocols were diverse but were generally well-tolerated and may confer many health advantages to older adults. Larger studies and more research in clinical populations most representative of older adults are needed to further evaluate the clinical effects of HIIT in these groups.
The effect of ethanolic leaves extract of Hymenodictyon floribundun on inflammatory biomarkers: a data-driven approach
Authors: Usman Abdullahi Garba , Ahmad Mubarak Hussaini , Danraka Rabi’u Nuhu , Abba Sani Isah 
Published: July 2021

Abstract
Background
Medicinal plants are used to manage pain and inflammatory disorders in traditional medicine. A scientific investigation could serve as a basis for the determination of molecular mechanisms of antinociceptive and antiinflammatory actions of herbal products. In this work, we used both artificial intelligence (AI) based models inform of adaptive neuro-fuzzy inference system and artificial neural network (ANN) as well as a linear model, namely; stepwise linear regression in modelling the performance of four different inflammatory biomarkers namely; interleukin (1L)-1β, 1L-6, tumour necrosis factor (TNF)-α and prostaglandin E2 (PGE2). This modelling was done using number of abdominal writes, the reaction time of paw licking in mice and paw oedema diameter as the input variables.

Results
Four different performance indices were employed, which are determination coefficient (DC), root mean squared error (RMSE), mean square error (MSE) and correlation co-efficient (CC). The results have shown the superiority of the AI-based models over the linear model.

Conclusions
The overall quantitative and visualized comparison of the results showed that adaptive neuro-fuzzy inference system outperformed the ANN and SWLR models in modelling the performance of the four inflammation biomarkers in both the calibration and verification phases.