bluuebunny/crossref_metadata_2025_split huggingface dataset:
DPP3 Expression Promotes Cell Proliferation and Migration in Vitro and Tumor Growth in Vivo That Associates with Poor Prognosis of Esophageal Carcinoma
Authors: Liu Jing-Kun , Abudula Abulizi , Yang Hai-Tao , Xu Li-Xiu , Bai Ge , Tulahong Aisiker , Eli Maynur 
Published: December 2021

Abstract
Background: Dipeptidyl peptidase III (DPP3) is a zinc-dependent metallopeptidase and elevated in a variety of malignant tumors, but the underlying mechanism is not well understood so far. Here we investigated the association of esophageal carcinogenesis with the regulation of DPP3 expression by tissue-based quantitative analysis and the depletion of DPP3 expression in esophageal cancer cells and xenograft model. Methods: The expression level of DPP3 in esophageal cancer tissues and adjacent normal tissues was detected in 93 cases of tissue biopsies collected from patients diagnosed with esophageal carcinoma by immunohistochemistry. The effect of DPP3 expression on cell proliferation, migration or apoptosis was determined in DPP3-depleted esophageal cancer cells created by infection with the lentivirus containing the shRNA specific to human DPP3 mRNA sequence followed by cytometric detection using celigo cell count assay, flow cytometry, wound-healing assay and trans-well assay as well as chip screening with a Human Apoptosis Antibody Array kit, which enables the quantitative detection of 43 apoptosis-related genes. A xenograft model was applied to the detection of tumor growth and invasion of DPP3-depleted cancer cells in nude mice.Results: DPP3 expression was elevated in esophageal cancer tissues compared with adjacent non-tumor tissues (normal controls) with statistical significance (P<0.05), and associated with poor prognosis of esophageal carcinoma. The DPP3-depletion resulted in a reduced cell proliferation and migration and enhanced cell-cycle arrest and apoptosis of esophageal cancer cells, and lead to the inhibition of tumor growth and invasion in xenograft model. In addition, DPP3-depletion was associated with the upregulation of pro-apoptotic proteins and the downregulation of anti-apoptotic proteins.Conclusions: These findings suggest that DPP3 may promote cell proliferation, migration and survival of esophageal cancer cells in vitro, and tumor growth and invasion of esophageal carcinoma in vivo and this might serve as a molecular target for tumor therapy.
Technical Analysis and Identification of a Glass Inlaid Sui-Tang Dynasty Woman’s Crown
Authors: Du Jingnan , Eckfeld Tonia , Yang Junchang , Jiang Fengrui , Zhang Quanmin , Shao Yanbing 
Published: December 2021

Abstract
A woman’s crown made of fine gilt bronze with refined glass inlay work was excavated in 2007 from the Sui-Tang tomb called Kunlun M2 in Xi’an. Many questions surround this mysterious crown which was found buried without context. This study uses nondestructive analysis of the crown to determine its significance, when was it made, and the status of the woman for whom was it made. Optical microscopy (OM), optical coherence tomography (OCT), micro X-ray fluorescence imagery (XRF), and Raman spectroscopy were applied, and identified the crown’s inlays as potash-lime glass, composed mainly of the raw materials include vein quartz or quartzite with potassium nitrate as flux. The inlays were sintered before embedding into gilt copper wire filigree. Compared with existing research on other crowns and their inlays, it is speculated that the crown dates from the Sui dynasty (581-618) or early Tang dynasty (618-649), was made locally by Chinese craftsmen and belonged to the wife of an official.
Effects of Nipping and Micronutrient Foliar Application on Dry Matter Production, Yield, Agronomic Efficiency and Nutrient Uptake of Short Duration Pigeonpea (Cajanus Cajan L.) Co(Rg)- 7
Authors: G Srinivasan , R Gobi , Balasubramanian A. , Sathiyamurthi S. 
Published: December 2021

Abstract
Background The productivity of pulse crop is low due to cultivation on agriculturally marginal and sub marginal lands under poor management. So, it needs earnest attention in adaption of desirable production technologies to exploit the yield potential of the pulses and it can be possible by application of fertilizers, nipping and foliar application of nutrients.In this view, aresearch was conducted to studyyield maximization, agronomic efficiency and nutrient uptake pattern with nipping and nutrient management practices in irrigated pigeonpea.Methods Field experiment was conducted at farmer’s field, Palacode, Palacode Taluk, Dharmapuri District.The pigeonpea seeds were sown by adapting a spacing of 45 × 30 cm. Entire dose of N, P2O5 and K2O (25: 50: 25 kg)were applied basally and the foliar spraying of 0.5 per cent Micronutrient mixture was done as per treatment schedule on 30th& 45th days after sowing using Knapsack Sprayer with the spray fluid of 500 lit. ha-1.Results The results revealed that the application of T9 (125% RDF + Nipping + Micronutrient) recorded significantly higher dry matter production, yield and nutrient uptake.The least values were recorded under the treatment T1 (Control).
Chemical Characterization, Source Apportionment, and Health Risk Assessment of PM2.5 in a Typical Industrial Region in North China
Authors: wang zhanshan , Yan Jiayi , Zhang Puzhen , Li Zhigang , Guo Chen , Wu Kai , Li Xiaoqian , Zhu Xiaojing , Sun Zhaobin , Wei Yongjie 
Published: December 2021

Abstract
To clarify the chemical characteristics, source contributions, and health risks of pollution events associated with high PM2.5 in typical industrial areas of North China, manual sampling and analysis of PM2.5 were conducted in the spring, summer, autumn, and winter of 2019 in Pingyin County, Jinan City, Shandong Province. The results showed that the total concentration of 29 components in PM2.5 was 53.4 μg·m-3; the largest contribution was from the NO3- ion, at 14.6 ± 14.2 μg·m-3, followed by organic carbon (OC), SO42-, and NH4+, with concentrations of 9.3 ± 5.5, 9.1 ± 6.4, and 8.1 ± 6.8 μg·m-3, respectively. The concentrations of OC, NO3-, and SO42- were highest in winter and lowest in summer, whereas the NH4+ concentration was highest in winter and lowest in spring. Typical heavy metals had higher concentrations in autumn and winter, and lower concentrations in spring and summer. The annual average sulfur oxidation rate (SOR) and nitrogen oxidation rate (NOR) were 0.30 ± 0.14 and 0.21 ± 0.12, respectively, with the highest SO2 emission and conversion rates in winter, resulting in the SO42- concentration being highest in winter. Although the emission rate of SO2 was low in summer, its conversion rate was high. In winter and autumn, NORs were significantly higher than in spring and summer, and a higher NOR in autumn contributed to significant elevation of the NO3- concentration in autumn relative to spring and summer. The average concentration of secondary organic carbon in 2019 was 2.8±1.9 μg·m-3, and it comprised approximately 30% of total OC. The health risk assessment for typical heavy metals showed that Pb poses a potential carcinogenic risk for adults, whereas As may pose a carcinogenic risk for adults, children, and adolescents. Positive matrix factorization analysis indicated that coal-burning emissions contributed the largest fraction of PM2.5, accounting for 35.9% of the total. The contribution of automotive emissions is similar to that of coal, at 32.1%. The third-largest contributor was industrial sources, which accounted for 17.2%. The contributions of dust and other emissions sources to PM2.5 were 8.4% and 6.4%, respectively.
Effects of Moringa Oleifera Leaf Polysaccharide on Growth Performance and Immune Response of Broiler Chickens
Authors: Husien Hosameldeen Mohamed , Huang JunJie , Peng WeiLong , Zheng ShuMei , Li JinGui 
Published: December 2021

Abstract
Moringa oleifera (MO) is a widely used as the nutritious and non-traditional feed supplementation containing kinds of bioactive substances. However, the enhancement effect of Moringa oleifera leaf Polysaccharide (MOLP) as a feed additive in broilers growth performance and immunity remains unclear. In this study, MOLP was obtained by water extraction and alcohol precipitation method, then purified with Trichloroacetic acid (TCA) assay. Chickens were randomly divided into 4 groups, to receive different doses of MOLP (0, 0.1, 0.2, 0.4g/kg) in feed for 3 weeks. The body weight gain (BWG) and feed consumption were recorded for feed conversion ratio (FCR) and average daily feed intake (ADFI) calculation. Broiler chickens were sacrificed and sampled on day 14, 21, 28 (D 14, D 21, and D 28) respectively. Serological indicators, including total protein (TP), albumin (ALB), globulin (GLO), and creatinine (CREA) were detected. ELISA kits were applied for detecting the levels of immunoglobulin A (IgA), immunoglobulin G (IgG), interleukin-2 (IL-2), and tumor necrosis factor (TNF-α). From D 21 to D 28, the results showed that middle dose of MOLP significantly increased BWG and ADFI as well as liver and bursa indexes when compared with the control group. In addition, TP and GLO were also increased (P<0.05). All MOLP treatments enhanced the serum concentrations of IgG and IL-2 (P<0.01). Furthermore, results of quantitative RT-PCR showed that high dose of MOLP treatment significantly increased (P<0.001) the mRNA expression levels of IL-2 and TNF-α of chickens relative to the control group. In conclusion, the results showed that MOLP supplementation contributed to improve growth performance and immune response in broiler chickens, and MOLP could be considered as a promising feed additive.
Bayesian Additive Modeling of Maternal Mortality In Ghana
Authors: Jakperik Dioggban , Nadarajah Saralees , Adjabui Michael Jackson 
Published: December 2021

Abstract
Maternal mortality is a critical measure for quality of health system in any country and hence many countries have made concerted efforts to check its occurrence. Various stakeholders involved in the management of health system in Ghana have been tasked to ensure women do not die whilst giving birth. This study was conducted on a sample of 1,052 women selected from all the ten administrative regions of Ghana in which 188 maternal deaths occurred. Two main analytical tools, namely the Zero-Inflated Negative Binomial Regression and Bayesian Additive Posterior Modeling using IN LA were used. Age at death, Marital Status, and Place of Death emerged as the most significant determinants of maternal mortality in Ghana. It was realized that high number of maternal deaths were recorded in the least developed regions with Northern Region having the highest number of Maternal Deaths. It is therefore important for stakeholders to devise a road map of getting health workers to accept postings to the rural areas and also provide well resourced health facilities to stem this menace.
In Vitro Rumen Degradability of Tropical Legumes and Their Secondary Metabolites Depends on Inoculum Source
Authors: Ortega Einar Artiles , Fé-Rodríguez Pedro Yoelvys de la , Reguera-Barreto Beydis , Lima-Orozco Raciel , Fievez Veerle 
Published: December 2021

Abstract
In this study, the in vitro apparent rumen degradability of organic matter (ARDOM) and plant secondary metabolites (ARDPSM) of three tropical legumes (Mucuna pruriens, Canavalia ensiformis and Leucaena leucocephala) were assessed. For this, single end-point incubations (24 h) as well as kinetic assessments (0, 2, 4, 6, 8, 10, 12 and 24 h) were set up with different inocula sources, i.e. ruminal inocula from Belgian and Cuban sheep. L-mimosine, L-canavanine, Concanavaline A (Con A) and trypsin inhibitor (TI) were the plant secondary metabolites (PSM) targeted in this study. Bean and forage/bean meals of M. pruriens and C. ensiformis as well as their PSM were extensively degraded during 24 h incubation (0.70 and 0.78 g ARDOM/g of organic matter (OM), respectively and > 0.80 g/g for L-canavanine, > 0.76 TIU/TIU for TI and > 0.95 g/g for Con A, for both legumes). Forage meal of L. leucocephala was considerably less degraded, with apparent ruminal degradabilities of 0.20 and 0.35 g ARDOM/g OM after 24 h incubation with Belgian or Cuban sheep inoculum, respectively. This could - at least partially - be related to L-mimosine, present in L. leucocephala, which was hardly degraded in the Belgian incubation, while a more extensive ruminal breakdown was observed under the Cuban conditions (0.05 to 0.78 g ARDPSM/g PSM, respectively). The negative effect of L-mimosine on OM degradability was supported by in vitro incubations with straw and inoculum from Belgian sheep, as ruminal degradation of straw was 31% lower when L-mimosine was supplemented.
Heat Shock Protein Grp78/BiP/HspA5 Binds Directly to TDP-43 and Mitigates Toxicity Associated with Neurodegenerative Disease Pathology
Authors: François-Moutal Liberty , Scott David , Ambrose Andrew , Zerio Christopher , Dissanayake Kumara , May Danielle , Carlson Jacob , Barbieri Edward , Moutal Aubin , Roux Kyle , Shorter James , Khanna Rajesh , Barmada Sami , McGurk Leeanne , Khanna May 
Published: December 2021

Abstract
Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disease with no cure or effective treatment in which TAR DNA Binding Protein of 43 kDa (TDP-43) abnormally accumulates into misfolded protein aggregates in affected neurons. It is widely accepted that protein misfolding and aggregation promote proteotoxic stress. The molecular chaperones are the body’s primary line of defense against proteotoxic stress and there has been long-standing interest in understanding the relationship between chaperones and aggregated protein in ALS. Of particular interest are the heat shock protein of 70 kDa (Hsp70) family of chaperones; however, defining which of the 13 human Hsp70 isoforms is critical for ALS, has presented many challenges. To gain insight into the specific Hsp70 that modulates TDP-43, we investigated the relationship between TDP-43 and the Hsp70s using proximity-dependent biotin identification (BioID) and discovered several Hsp70 isoforms associated with TDP-43 in the nucleus, raising the possibility of an interaction with native TDP-43. We further found that HspA5 bound specifically to the RNA-binding domain of TDP-43 using recombinantly expressed proteins. HspA5 is increased in prefrontal cortex neurons of ALS patients. Finally, overexpression of HspA5 in Drosophila rescued TDP-43-induced toxicity, suggesting that upregulation of HspA5 may have a compensatory role in ALS pathobiology.
Contact Toxicity of Insecticides and Biopesticides to Trichogramma Chilonis Ishii. (Hymenoptera: Trichogrammatidae) under Laboratory Condition
Authors: Nidagundi Kartik S , Kambrekar DN , Mallapur C. P. 
Published: December 2021

Abstract
Integration of insecticides and biological controls is an important tactic of Integrated Pest Management (IPM). Trichogramma chilonis is a promising natural enemy of many lepidopteran insect pests. However, this hymenopteran egg parasitoid is adversely affected by most insecticides. Contact toxicity of nineteen insecticides and three biopesticides on adults of T. chilonis was investigated by using dry film residue bioassays under laboratory conditions. Profenofos and chlorpyrifos were highly lethal to the adults even at sublethal doses followed by dimethoate, spinosad, indoxacarb and acephate + imidacloprid. Diafenthiuron, nimbecidine and flubendiamide were categorised as less toxic. The biopesticides viz., Metarhizium anisopliae, Beauveria bassiana and Metarhizium rileyi were found innocuous to T. chilonis. The studysuggests that the insecticides with less toxicity and biopesticides with apparently no harmful effects on the parasitoid can be used in conjunction with parasitoids in IPM programmes. This will also advice the plant protectionists in avoiding the one with detrimental effects on this hymenopteran wasp with appropriate timing of application that controls the pests without adversely affecting their natural enemies
The Effect of Etanercept Therapy on Adrenal Steroid Metabolism in Juvenile Idiopathic Arthritis: A Steroid Metabolomics Approach
Authors: Aviel Yonatan Butbul , Keinan Ariel , Hartmann Michaela , Wudy Stefan , Tiosano Dov 
Published: December 2021

Abstract
Objective
To evaluate the impact of anti-tumor necrosis factor-alpha (TNFα: etanercept [Enbrel®]) on adrenal activity in the acute phase of juvenile idiopathic arthritis (JIA) during which there is a decrease in adrenal androgen and cortisol levels.
Method
Eleven JIA patients aged 12±6.2 years with a disease duration of 6.3±5.2 years were enrolled. They were treated once weekly with etanercept (0.8 mg/kg) for 3±2.8 years. Urine samples for gas chromatography-mass spectrometry steroid hormone analysis were collected before, and 1 and 3 days after etanercept injection and compared to age- and sex-matched healthy control.
Results
The levels of 23 of the 35 metabolites were low before etanercept treatment. Those 23 metabolites included 2 C19 steroids (androgens), 3 C21 steroid hormone intermediates, 14 cortisol metabolites, 2 corticosterone metabolites and 2 C18 steroids (estrogens). One day post-treatment, only 5 of the 23 metabolite levels remained low. They included 2 C19 metabolites, 2 21 steroid metabolites and 1 cortisol metabolite b-c. Three days post-treatment, the only metabolite levels that continued to be low were 1 C19 metabolite, 2 C21 steroid hormone intermediates and 1 cortisol metabolite (a-Cl), while the remaining 18 metabolites had already normalized after 1 day. DHEAS and 17 OH pregnenolone metabolite levels were the last ones to recover. Urinary metabolite ratios reflecting CYP21 and 11β-HSD2 enzymatic activities were lower in JIA patients than in controls.
Conclusion
Almost all of the pre-etanercept treatment cortisol urinary metabolite levels were significantly lower than normal, and almost all rose to normal values by 1 day post-treatment. Blocking TNFα restores adrenal function in JIA.
3D Geological Implicit Modeling Method of Regular Voxel Splitting Based on Layered Interpolation Data
Authors: li jian , liu pei-rong , wang xinyu , cui hao , ma yurong 
Published: December 2021

Abstract
In view of the problems in traditional geological modeling methods, such as the insufficient utilization of geological survey data, the inaccurate expression of a stratigraphic model, and the large amount of model data, a 3D geological model cannot be smoothly loaded and rendered on the web end. In this paper, a 3D geological implicit modeling method of regular voxel splitting based on hierarchical interpolation data is proposed. This method first uses the boreholes and geological section data from a geological survey for data conversion and fusion, compares the applicability of different interpolation algorithms through cross-validation research, and uses the best fitting algorithm to interpolate and encrypt discrete points in the formation. Then, it constructs the regular voxels, designs five different regular voxel split types, and divides the voxels. In addition, the data structure design of the voxel split model is implemented, and the irregular voxel metadata structure is analyzed and displayed through Three.js. Using this method, based on the survey data of an area in Zhengzhou, the global workflow from data processing to model construction and visualization is demonstrated. The experimental results show that the model can integrate multisource hierarchical interpolation data; express different stratum structures accurately and smoothly, and can realize the fast rendering, spatial query and analysis of the internal information of a geological body in a browser.
Intraoperative Measurements of Nerve Root Tension During Posterior Lumbar Interbody Fusion
Authors: Fu Zhi-yi , Wu Yujie , Zhu Tong 
Published: December 2021

Abstract
Background

There is no effective standard method to evaluate whether the nerve root tension is restored, which is an important indicator for the recovery of nerve function. This study aimed to demonstrate a technique for measuring nerve root tension during surgery.
Methods

A total of 54 consecutive patients (average age, 52.3 years; range, 28-68 years) received posterior lumbar interbody fusion for lumbar disc herniation comprised the patient sample.The nerve root tension was measured twice before and after intraoperative decompression by the nerve root tension meter modified from the transverse gauge by author. Clinical outcome was assessed by the visual analog scale (VAS) for leg pain, provided by patients before and after surgery.
Results

There was a significant improvement in the VAS score for leg pain after surgery compared with that before surgery (7.0 ± 2.24 vs. 0.8 ± 0.84, respectively; P < 0.01). Nerve root tension was significantly decreased after decompression compared with that before surgery (1.32 ± 0.22 N vs. 0.64 ± 0.17 N, respectively; P < 0.01). The nerve root tension was positively correlated with the VAS score (r = 0.772, P < 0.05; r = 0.715, P < 0.05).
Conclusions

This study shows that the nerve root tension meter can instantly and non-invasively measure nerve root tension during an operation. It was demonstrated that the nerve root tension of the patient is significantly reduced after decompression. Meanwhile, the VAS score improved significantly, and the nerve root tension and VAS scores were positively correlated.
Diagnosis and Psychotherapeutic Needs by Freiburg Personality Inventory in Inflammatory Bowel Disease
Authors: Rada Cornelia , Gheonea Dan , Popa Denisa Elena , Tieranu Cristian George 
Published: December 2021

Abstract
Background Despite recent discoveries in pharmacotherapy and psychotherapy, patients with inflammatory bowel disease (IBD) still face challenges with improvement, remission, and healing. The objectives of the study were to identify the characteristics of patients with IBD with the Freiburg Personality Inventory and the intensity of the colonic disease, comorbidities, and other factors that could be related to the personality of the subjects.Methods Data were collected in the period 2019–2020 from 46 patients from records at two hospitals. This study used nonparametric methods, such as the Wilcoxon-Mann–Whitney, Kolmogorov–Smirnov, Mann–Whitney U test, and BOOTSTRAP method. The control group was considered the national normative sample based on the average raw scores on the 12 personality assessment scales using the t test (one sample t test).Results Compared to the control group, the Inhibitedness, Health Concerns, and Emotionality scales had significantly higher raw scores. Additionally, the Social Orientation, Frankness, and Extraversion scales had significantly lower raw scores. Health status was a medical factor that influenced personality scales. On the Somatic Complaints scale, patients who had lesions or comorbidities had significantly higher raw scores. Patients who had comorbidities in addition to IBD had considerably higher raw scores on the Excitability scale.Conclusions Positive affective and cognitive adjustment interventions may aid people with IBD in managing life problems in a balanced manner while interpreting treatment outcomes with confidence. Psychotherapeutic change interventions regarding life perception are required to tackle the description of subjective suffering related to physical inconveniences (Somatic Complaints scale), a strong orientation toward performance (Strain scale), mood swings, anxiety, and pessimism (Emotionality scale). Another intervention is reconsidering values and (re)prioritization, such as family, intimate relationships, friends, health, growth, development, balanced work, all of which can promote a feeling of well-being and balance.