bluuebunny/crossref_metadata_2025_split huggingface dataset:
Observational Study of Nisarg Cyclone Winds using Phased Array Doppler Sodar at Atigre, Kolhapur, India
Authors: Pawar Rani P. , dharmaraj Thiyagesan , Nade Dada P. , Patil Mahendra N. , Patil Omkar M. , Victor Jeni , Pawar S. D. 
Published: November 2021

Abstract
One of the most important parameters in meteorology is the mean wind profile in the tropical cyclone boundary layer. The signature of the Nisarg cyclone is reported in the Phased Array Doppler Sound Detection and Ranging (SODAR) data installed at the Center for Space and Atmospheric Science (CSAS), Sanjay Ghodawat University, Kolhapur (16.74° N, 74.37° E; near India's western coast). The vertical profile of wind speed and wind direction measured from the sodar system clearly reveals the signature of Nisarg cyclone during 2- 3 June 2020. Our analysis revealed that, the maximum mean wind speed was 17 m/s on 3rd June 2020 at 10:00 IST. It also shows the change in the wind direction from southwest to southeast on 2nd June 2020 and 3rd June 2020. Daily high-resolution reanalysis in the domain, 0-25°N, 65-110°E, during the period from 31st May-5th June 2020 shown the variation in atmospheric pressure of the Nisarg cyclone from 1000 to 1008 hPa, sea surface tremperature (SST) between 30 and 31°C, outgoing longwave radiation (OLR) varied between 100 and 240 Wm−2, wind speed between 3 and 15 m/s and low values of vertical wind shear (VWS) was observed to the north of the track Nisarg. These findings could aid in better understanding and forecasting in this region. The present results are initial measurements of sodar system.
Process Related Characteristic-Based Topography Evaluation of Wear Conditions On Grinding Wheels
Authors: Strug Maikel , Denkena Berend , Breidenstein Bernd , Krödel-Worbes Alexander 
Published: November 2021

Abstract
Non-productive auxiliary processes affect the single part and small badge production of milling tools. The key production process grinding is inevitably linked to the auxiliary conditioning process. The time demand of those process steps decreases the overall productivity of the manufacturing process. However, today the machine operator decides on conditioning cycles individually by the use of experience. Until today, there is no objective data based approach available that supports the initiation of these conditioning processes or the adaption of the grinding process itself in order to improve its process efficiency. For this purpose, a process related topography evaluation method of the grinding wheel surface is developed within this study. For the measurement an optical method based on laser triangulation is used. The measurement system is implemented into a common tool grinding machine tool. In addition, characteristic topography values are defined that show the wear conditions of the grinding tool. Moreover, the data is summarized in a database of wear conditions. The developed measurement method can save grinding and dressing tool resources, process times and minimizes scrap parts. In addition, an adaptation of the process and a targeted launch of auxiliary processes can be enabled. The novel characteristic-based topography measurement creates the opportunity to enhance the tool life of the grinding wheels up to 30 % without losing productivity.
Operational Behavior of Graded Diamond Grinding Wheels For End Mill Cutter Machining
Authors: Denkena Berend , Bergmann Benjamin , Raffalt Daniel 
Published: November 2021

Abstract
The varying related material removal rate during deep grinding of cemented carbide end mill cutters results in an unevenly wear of the grinding wheel. This causes a reduced geometrical precision of the manufactured tools. Consequently, the intervals between dressing steps are reduced and the dressing infeed increases. The aim of this research project is therefore to design a tailored grinding tool with uniform wear behavior. To address this situation, the grinding tool load is determined simulatively along the width of the grinding wheel. From this an equation is derived to adapt the bonding layer properties to the local load differences. First investigations show that two different concentrations zones in the abrasive layer of the grinding wheel improves the wear behavior already. This indicates that a further reduction of the wear difference is possible by a more uniform gradient. A simulation is performed to define a knowledge based gradient with more than two concetration zones. This allows a more precise load optimized adaptation of the grinding layer properties to the geometry to be ground in terms of wear behavior. Grinding tools manufactured on this basis are used for flute grinding of end mill cutters. A reduction of the wear difference over the grindig wheel width of 52% and an improved cutting edge quality of these are demonstrated.
Comparison of The Electrophysiological Characteristics of Tight Filum Terminale And Tethered Cord Syndrome
Authors: Kamei Naosuke , Nakamae Toshio , Nakanishi Kazuyoshi , Morisako Taiki , Harada Takahiro , Maruyama Toshiaki , Adachi Nobuo 
Published: November 2021

Abstract
This study aims to characterize tight filum terminale (TFT) in motor evoked potential (MEP) testing by comparing TFT patients with both tether cord syndrome (TCS) patients and healthy subjects. Fifty TFT patients, 18 TCS patients, and 35 healthy volunteers participated in this study. We recorded MEPs following transcranial magnetic stimulation from the bilateral abductor hallucis muscles as well as compound muscle action potentials and F-waves evoked by electrical stimulation of the tibial nerve from the bilateral abductor pollicis brevis muscles. The peripheral conduction time (PCT) was calculated from the latency of the compound action potential and F-wave. Furthermore, the central motor conduction time (CMCT) was calculated by subtracting PCT from MEP latency. TFT and TCS patients had a significantly longer MEP latency than healthy subjects. PCT in TFT patients were significantly longer than those in TCS patients or healthy subjects. Using the cut-off values for PCT, we were able to diagnose patients with TFT patients with a sensitivity of 72.0% and a specificity of 91.4%. Prolonged PCT in the MEP test may be a useful indicator for TFT and suggests that MEP may be used as an adjunct diagnostic tool for TFT.
Oncogenic RAS Generates Cancer Stem Cells in p53-Deficient Fibroblasts Through SOX2 Induced By CDK1-Mediated Protein O-GlcNAcylation
Authors: Shimizu Masahiro , Shibuya Hiroshi , Tanaka Nobuyuki 
Published: November 2021

Abstract
Cancer stem cells (CSCs) have tumour initiation, self-renewal, and long-term tumour repopulation properties, and it is postulated that differentiated somatic cells can be reprogrammed to CSCs by oncogenic signals. We previously showed that oncogenic HRASV12 conferred tumour initiation capacity in tumour suppressor p53-deficient (p53−/−) primary mouse embryonic fibroblasts (MEFs) through transcription factor NF-κB-mediated enhancement of glucose uptake; however, the underlying mechanisms of RAS oncogene-induced CSC reprogramming have not been elucidated. Here, we found that the expression of the reprogramming factor SOX2 was induced by HRASV12 in p53−/− MEFs. Moreover, gene knockout studies revealed that SOX2 is an essential factor for the generation of CSCs by HRASV12 in mouse and human fibroblasts. We demonstrated that HRASV12-induced cyclin-dependent kinase 1 (CDK1) activity and subsequent enhancement of protein O-GlcNAcylation were required for SOX2 induction and CSC generation in these fibroblasts and cancer cell lines containing RAS mutations. Moreover, the CDK inhibitor dinaciclib and O-GlcNAcylation inhibitor OSMI1 reduced the number of CSCs derived from these cells. Taken together, our results reveal a signalling pathway and mechanism for CSC generation by oncogenic RAS and suggest the possibility that this signalling pathway is a therapeutic target for CSCs.
The Role of MicroRNA-155 in Glomerular Endothelial Cell Injury of Diabetic Nephropathy
Authors: He Kaiying , Chen Zhan , Zhao Jing , He Yang , Deng Rongrong , Fan Xin , Zhou Xiaochun , Wang Jianqin 
Published: November 2021

Abstract
Objective: To investigate the role of microRNA-155-5p (miR-155-5p) on apoptosis and inflammatory response in human glomerular endothelial cells (HRGEC) cultured with high glucose.Methods: The primary human glomerular endothelial cells (HRGEC) were studied, QPCR, WB , IF were used to detect cell morphology, target gene ETS-1 (ETS-1), downstream factors VCAM-1 and MCP-1, and apoptosis of cells in each group after high glucose stimulation and transfection with miR-155 overexpression or inhibitor.Results:1.The expression of inflammatory factors and apoptosis of HRGEC cells increased under high glucose stimulation.2.The overexpression of miR-155 in HRGEC cells under high glucose stimulation decreased the expression of ETS-1, while the expression of ETS-1 increased when miR-155 was inhibited. These results suggest that miR-155 may be involved in endothelial cell injury by negatively regulating the expression of ETS-1.3.HRGEC cells were transfected with miR-155 mimic and ETS-1 siRNA with high glucose stimulation. The expression of ETS-1 was positively correlated with the expression of downstream factors VCAM-1 and MCP-1. These results suggest that ETS-1 can mediate endothelial cell inflammation by regulating VCAM-1 and MCP-1.
Evolution Behaviour of Kink Breathers and Lump-M-Solitons (M → ∞) for the (3+1)-Dimensional Hirota-Satsuma-Ito-Like Equation
Authors: Li Long-Xing 
Published: November 2021

Abstract
In this paper, some novel lump solutions and interaction phenomenon between lump and kink M-soliton are investigated. Firstly, we study the evolution and degeneration behaviour of kink breather wave solution with difffferent forms for the (3+1)-dimensional Hirota-Satsuma-Ito-like equation by symbolic computation and Hirota bilinear form. In the process of degeneration of breather waves, some novel lump solutions are derived by the limit method. In addition, M-fifissionable soliton and the interaction phenomenon between lump solutions and kink M-solitons (lump-M-solitons) are investigated, the theorem and corollary about the conditions for the existence of the interaction phenomenon are given and proved further. The lump-M-solitons with difffferent types is studied to illustrate the correctness and availability of the given theorem and corollary, such as lump-cos type, lump-cosh-exponential type, lump cosh-cos-cosh type. Several three-dimensional fifigures are drawn to better depict the nonlinear dynamic behaviours including the oscillation of breather wave, the emergence of lump, the evolution behaviour of fission and fusion of lump-M-solitons and so on.
Co-expression Effect of LLCL2 and SLC7A5 to Predict Prognosis in ERα-Positive Breast Cancer
Authors: Hisada Tomoka , Kondo Naoto , Wanifuchi-Endo Yumi , Osaga Satoshi , Fujita Takashi , Asano Tomoko , Uemoto Yasuaki , Nishikawa Sayaka , Katagiri Yusuke , Terada Mitsuo , Kato Akiko , Sugiura Hiroshi , Okuda Katsuhiro , Kato Hiroyuki , Komura Masayuki , Morita Satoshi , Takahashi Satoru , Toyama Tatsuya 
Published: November 2021

Abstract
Lethal giant larvae homolog 2 (LLGL2) and solute carrier family 7 member 5 (SLC7A5) have been reported to be involved in resistance to endocrine therapy. This study aimed to assess the effects of LLGL2/SLC7A5 co-expression in predicting prognosis and response to endocrine therapy in ERα-positive breast cancer patients by using LLGL2/SLC7A5 mRNA and protein expression in long-term follow-up invasive breast cancer tissues. We identified that low LLGL2/SLC7A5 mRNA co-expression (LLGL2low/SLC7A5low) was associated with disease-free survival (DFS) compared with other combination groups in all breast cancer patients. In ERα-positive breast cancer patients, LLGL2low/SLC7A5low showed longer DFS and overall survival (OS) compared with LLGL2high/SLC7A5high and a positive trend of longer survival compared with other combination groups. We also observed that LLGL2low/SLC7A5low showed longer survival compared with LLGL2high/SLC7A5high in ERα-positive breast cancer patients receiving adjuvant tamoxifen therapy. Multivariate analysis demonstrated that LLGL2low/SLC7A5low was an independent favorable prognostic factor of both DFS and OS, not only in all breast cancer patients, but also in ERα-positive breast cancer patients. High co-expression of LLGL2 and SLC7A5 protein showed a positive trend of shorter survival. Our study showed that co-expression of LLGL2 and SLC7A5 mRNA is a promising candidate biomarker in early breast cancer patients.
A Precursory Signal of the Central Pacific El Niño Event: Eastern Pacific Cooling Mode
Authors: Lingjiang Tao , Wansuo Duan 
Published: November 2021

Abstract
In recent decades, the tropical Pacific frequently experiences a new type of El Niño with warming center in the central tropical Pacific (i.e., the CP-El Niño) with distinct global climate effect to the traditional El Niño (i.e., EP-El Niño). Predicting the El Niño diversity is still a huge challenge for climatologists partly due to the precursory signals of El Niño events with different type is unclear. In the present study, a novel precursory signal that presents a negative sea surface temperature anomaly in the eastern tropical Pacific (i.e., EP-cooling mode) is revealed, which tends to evolve into a CP-El Niño event. The transition from the EP-cooling mode to CP-El Niño is explained by the basin-scale air-sea coupling in the tropical Pacific and teleconnections between the tropical and North Pacific. With the EP-cooling mode as a predictor, the forecast skill for the CP-El Niño in hindcast experiments is obviously improved by using regression models. The results in the present study are therefore instructive for promoting a better understanding of El Niño diversity and predictability.
Characterization of Globodera ellingtonae Populations from Chile Utilizing Whole Genome Sequencing
Authors: Hesse C.N. , Moreno I. , Acevedo Pardo O. , Pacheco Fuentes H. , Grenier E. , Dandurand L. M. , Zasada I. A. 
Published: November 2021

Abstract

Globodera ellingtonae was originally described from populations collected in the United States. In the original description, ribosomal DNA loci from Globodera sp. collected in Chile and Argentina were similar to G. ellingtonae, suggesting this nematode originated in this region of South America. In an effort to find additional populations of G. elllingtonae, collection trips were conducted in 2017 and 2020 in the Antofagasta and Arica y Parinacota Regions in Northern Chile, respectively. Globodera sp. were more prevalent in Antofagasta (17 samples collected, 53% positive for Globodera sp.) than in Arica y Parincota (16 samples collected, 13% positive for Globodera sp.). The genomes of single cysts (N ≥ 3) from four fields were sequenced. Additionally, the genomes of the G. ellingtonae population from Oregon and a Globodera sp. population originally collected in Antofagasta Region but maintained in culture in France were also sequenced. Based upon a HSP90 sequenced data mined from WSG data, all of the populations from the Antofagasta Region were G. ellingtonae and grouped in a monophyletic clade. A population collected from the Arica y Parincota Region was identified as G. rostochiensis based upon HSP90 data. Genome-wide SNP patterns of the G. ellingtonae populations showed strong clustering based on geographic location indicating that G. ellingtonae has high genetic diversity within Chile. A phylogenetic tree derived from 168,354 binary SNPs in the nuclear genome showed separate but distinct clustering of the Oregon population and the population from Antofagasta maintained in France. The Oregon G. ellingtonae population subtended the Chilean clades and placed on a long branch representing approximately twice the genetic variation observed among all Chilean G. ellingtonae populations. The possibility remains that G. ellingtonae from Chile may be sufficiently diverged to constitute a new species from G. ellingtonae originally described from a population collected in Oregon.
Announcement of WormAtlas partnership with the Journal of Nematology
Authors: Schroeder Nathan E. , Hall David H. 
Published: November 2021

Abstract
A detailed understanding of nematode anatomy can be leveraged for the development of new parasitic nematode control strategies and for fundamental biological insights through nematode model organisms. The Center for C. elegans Anatomy, with its websites WormAtlas and WormImage, is the central anatomical resource for researchers studying the model organism Caenorhabditis elegans. Here, we announce our expansion of the WormAtlas and WormImage resources beyond C. elegans to include additional nematode species. Towards this goal, we will partner with the Journal of Nematology to write and solicit anatomically focused review chapters for publication in the Journal and corresponding inclusion on the WormAtlas website.
A Significant Role of Chemistry in Drug Development: A Systematic Journey from Traditional to Modern Approaches with Anti-HIV/AIDS Drugs as Examples
Authors: Yadav Madhu , Srivastava Ritika , Naaz Farha , Verma Rajesh , Singh Ramendra K. 
Published: November 2021

Background:
Traditionally, various plant extracts having interesting biological properties were the
main source of new drugs. In the last 30 years, the role of chemistry in combination with new technologies, like
various computational techniques in chemistry, has witnessed a major upsurge in drug discovery and targeted
drug delivery.

Objective:
This article provides a succinct overview of recent techniques of chemistry that have a great impact
on the drug development process in general and also against HIV/AIDS. It focuses on new methods employed
for drug development with an emphasis on in silico studies, including identifying drug targets, especially the
proteins associated with specific diseases.

Methods:
The rational drug development process starts with the identification of a drug target as the first phase,
which helps in the computer-assisted design of new drug molecules. Synthetic chemistry has a major impact on
the drug development process because it provides new molecules for future study. Natural products based semisynthesis
or microwave assisted synthesis is also involved in developing newly designed drug molecules. Further,
the role of analytical chemistry involves extraction, fractionation, isolation and characterization of newly
synthesized molecules.

Results:
Chemistry plays a key role in drug discovery and delivery by natural process or with the help of synthetic
nanoparticles or nanomedicines. So, nanochemistry is also deeply involved in the development of new
drugs and their applications.

Conclusion:
The previous era of drug discovery was dominated only by chemistry, but the modern approaches
involve a comprehensive knowledge of synthetic chemistry, medicinal chemistry, computational chemistry and
the concerned biological phenomenon.
Design Strategies, Chemistry and Therapeutic Insights of Multi-target Directed Ligands as Antidepressant Agents
Authors: Singh Karanvir , Bhatia Rohit , Kumar Bhupinder , Singh Gurpreet , Monga Vikramdeep 
Published: November 2021

:Depression is one of the major disorders of the central nervous system worldwide and causes disability and functional impairment. According to the World Health Organization, around 265 million people worldwide are affected by depression. Currently marketed antidepressant drugs take weeks or even months to show anticipated clinical efficacy but remain ineffective in treating suicidal thoughts and cognitive impairment. Due to the multifactorial complexity of the disease, single-target drugs do not always produce satisfactory results and lack the desired level of therapeutic efficacy. Recent literature reports have revealed improved therapeutic potential of multi-target directed ligands due to their synergistic potency and better safety. Medicinal chemists have gone to great extents to design multitarget ligands by generating structural hybrids of different key pharmacophores with improved binding affinities and potency towards different receptors or enzymes. This article has compiled the design strategies of recently published multi-target directed ligands as antidepressant agents. Their biological evaluation, structural-activity relationships, mechanistic and in silico studies have also been described. This article will prove to be highly useful for the researchers to design and develop multi-target ligands as antidepressants with high potency and therapeutic efficacy.
Ancestral Area Reconstruction of SARS-CoV-2 Indicates Multiple Sources of Entry into Australia
Authors: Phan Ngoc Minh Hien , Faddy Helen , Flower Robert , Spann Kirsten , Roulis Eileen 
Published: November 2021

Background:
The ongoing COVID-19 pandemic is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). International travels to Australia during the early stages of the pandemic prior to border closure provided avenues for this virus to spread into Australia. Studies of SARS-CoV-2 biogeographical distribution can contribute to the understanding of the viral original sources to Australia.

Objective:
This study aimed to investigate the clonality and ancestral sources of Australian SARS-CoV-2 isolates using phylogenetic methods.

Methods:
We retrieved 1,346 complete genomes from Australia along with 153 genomes from other countries from the GISAID and NCBI nucleotide databases as of the 14th May 2020. A representative dataset of 270 Australian and international sequences were resulted from performance of nucleotide redundancy reduction by CD-HIT. We then constructed a median-joining network by Network 10.1.0.0, and phylogenies by IQ-Tree, BEAST and FastTree. The Bayesian statistical dispersal-vicariance analysis (S-DIVA) and Bayesian interference for discrete areas (BayArea) built in RASP were used to reconstruct ancestral ranges over the phylogenetic trees.

Results:
Two major clusters, from Europe and from Asia, were observed on the network of 183 haplotypes with distinct nucleotide variations. Analysis of ancestral area reconstruction over the phylogenies indicated most Australian SARS-CoV-2 sequences were disseminated from Europe and East Asia-Southeast Asia.

Conclusion:
The finding is genetic evidence for the geographic origins of the Australian SARS-CoV-2 sequences. Most Australian sequences were genetically similar to those from Europe and East Asia-Southeast Asia, which were also suggested as two main sources of introduction of SARS-CoV-2 to Australia.