bluuebunny/crossref_metadata_2025_split huggingface dataset:
Association of malaria and curable sexually transmitted infections with pregnancy outcomes in rural Burkina Faso
Authors: Zango Serge Henri , Lingani Moussa , Valea Innocent , Samadoulougou Ouindpanga Sekou , Bihoun Biebo , Lankoande Diagniagou , Donnen Phillipe , Dramaix Michele , Tinto Halidou , Robert Annie 
Published: October 2021

Abstract
Background
Malaria and curable sexually transmitted infections (STIs) are severe infections associated with poor pregnancy outcomes in sub-Saharan countries. These infections are responsible for low birth weight, preterm birth, and miscarriage. In Burkina Faso, many interventions recommended by the World Health Organization were implemented to control the impact of these infections. After decades of intervention, we assessed the impact of these infections on pregnancy outcomes in rural setting of Burkina Faso.

Methods
Antenatal care and delivery data of pregnant women attending health facilities in 2016 and 2017 were collected in two rural districts namely Nanoro and Yako, in Burkina Faso. Regression models with likelihood ratio test were used to assess the association between infections and pregnancy outcomes.

Results
During the two years, 31639 pregnant women received antenatal care. Malaria without STI, STI without malaria, and their coinfections were reported for 7359 (23.3%), 881 (2.8 %), and 388 (1.2%) women, respectively. Low birth weight, miscarriage, and stillbirth were observed in 2754 (10.5 %), 547 (2.0 %), and 373 (1.3 %) women, respectively. Our data did not show an association between low birth weight and malaria [Adjusted OR: 0.91 (0.78 – 1.07)], STIs [Adjusted OR: 0.74 (0.51 – 1.07)] and coinfection [Adjusted OR: 1.15 (0.75 – 1.78)]. Low birth weight was strongly associated with primigravidae [Adjusted OR: 3.53 (3.12 – 4.00)]. Both miscarriage and stillbirth were associated with malaria [Adjusted OR: 1.31 (1.07 – 1.59)], curable STI [Adjusted OR: 1.65 (1.06 – 2.59)], and coinfection [Adjusted OR: 2.00 (1.13 – 3.52)].

Conclusion
Poor pregnancy outcomes remained frequent in rural Burkina Faso. Malaria, curable STIs, and their coinfections were associated with both miscarriage and stillbirth in rural Burkina. More effort should be done to reduce the proportion of pregnancies lost associated with these curable infections by targeting interventions in primigravidae women.
Digging down or scratching the surface: how patients use metaphors to describe their experiences of psychotherapy
Authors: Malkomsen A. , Røssberg J. I. , Dammen T. , Wilberg T. , Løvgren A. , Ulberg R. , Evensen J. 
Published: October 2021

Abstract
Background
In the present study, we wanted to explore which metaphors patients suffering from major depressive disorder (MDD) use to explain their experience of being in therapy and their improvement from depression.

Methods
Patients with MDD (N = 22) received either psychodynamic therapy (PDT) or cognitive behavioral therapy (CBT). They were interviewed with semi-structured qualitative interviews after ending therapy. The transcripts were analyzed using a method based on metaphor-led discourse analysis.

Results
Metaphors were organized into three different categories concerning the process of therapy, the therapeutic relationship and of improvement from depression. Most frequent were the metaphorical concepts of surface and depth, being open and closed, chemistry, tools, improvement as a journey from darkness to light and depression as a disease or opponent.

Conclusions
Patient metaphors concerning the therapeutic experience may provide clinicians and researchers valuable information about the process of therapy. Metaphors offer an opportunity for patients to communicate nuances about their therapeutic experience that are difficult to express in literal language. However, if not sufficiently explored and understood, metaphors may be misinterpreted and become a barrier for therapeutic change.

Trial registration
Clinical Trial gov. Identifier: NCT03022071. Date of registration: 16/01/2017.
Results of multiple ligament reconstruction after knee dislocation——A prospective study with 95 patients and minimum 2-year follow up
Authors: Li Tao , Xiong Yan , Zhang Zhong , Tang Xin , Chen Gang , Li Qi , Fu Wei Li , Li Jian 
Published: October 2021

Abstract
Background
There is still a lack of clinical data in arthroscopic treatment for treating multiple ligament injuries. This study aims to evaluate the clinical outcomes of patients with multiple ligament injuries undergoing treatment based on the classification stage and type of injury.

Methods
A prospective, clinical trial on multiple ligament injuries was planned, which included 95 patients (58 men and 37 women; age: 42.8 ± 11.9 [range, 18–63] years) from October 2017 to June 2018. Injuries were classified into three stages (emergency stage < 24 h; acute stage: 24 h to 3 weeks, and chronic stage: > 3 weeks) and six types (KD I–VI) based on injuries time and structures, which indicated appropriate treatments. The clinical outcomes were evaluated at 2, 4, 6, 8, and 12 weeks and at 6, 9, 12 months and 24 months after surgery. The final choices in efficacy index included International Knee Documentation Committee (IKDC) score, Lysholm score, visual analog scale (VAS) score, and range of motion.

Results
During the follow-up, all patients exhibited statistically significant functional improvement in the injured limb compared with their preoperative situation. The mean postoperative scores of acute stage patients at 2-year follow-up were IKDC subjective score, 77.54 ± 11.53; Lysholm score, 85.96 ± 9.39; Tegner score, 4.13 ± 1.08; and VAS score, 1.21 ± 0.76. The mean postoperative scores of chronic stage patents at 2-year follow-up were IKDC subjective score, 74.61 ± 12.38; Lysholm score, 81.71 ± 10.80; Tegner score, 3.96 ± 1.14; and VAS score, 1.71 ± 0.60. The IKDC subjective score, Lysholm score, and Tegner score were significantly improved (P < 0.01) and the VAS score was significantly decreased (P < 0.01) at 2-year follow-up. Regarding the multiple ligament injuries classification, patients with more structural damage in stages V and VI showed less progress in functional recovery than those in stages I–IV.

Conclusions
This new classification with three stages and six types helps to identify the severity of injury and plan the management effectively. The outcomes were encouraging and the subjective functional results showed significant improvement at 2-year follow-up.

Study design
Prospective clinical trial.

Level of evidence
II
Preparation of an anti-NEK2 monoclonal antibody and its application in liver cancer
Authors: Chen Qiuli , Li Hui , Yang Lichao , Wen Sha , Huang Xuejing , Liu Jiajuan , Guo Xiaoping , Hu Bing , Li Gang , He Min 
Published: October 2021

Abstract
Background
Never in mitosis gene-A (NIMA)-related expressed kinase 2 (NEK2) is a serine/threonine protein kinase regulated by the cell cycle. The purpose of this study was to obtain NEK2 protein to prepare an anti-NEK2 monoclonal antibody (mAb) and explore the application of the anti-NEK2 mAb of therapeutic and diagnostic in hepatocellular carcinoma (HCC).

Results
The NEK2 gene sequence was cloned from the normal liver cell line HL7702, and the full-length NEK2 gene sequence was cloned into the prokaryotic expression vector pET30a and transformed into Escherichia coli BL21 (DE3) cells. The recombinant fusion protein was obtained under optimized conditions and injected in BALB/c mice to prepare an anti-NEK2 mAb. By screening, we obtained a stable hybridoma cell line named 3A3 that could stably secrete anti-NEK2 mAb. Anti-NEK2 3A3 mAb was purified from ascites fluid. The isotype was IgG1, and the affinity constant (Kaff) was 6.0 × 108 L/mol. Western blot, indirect enzyme-linked immunosorbent assay (iELISA), immunofluorescence and immunocytochemical analyses showed that the mAb could specifically recognize the NEK2 protein. MTT assays showed that the mAb 3A3 could inhibit the proliferation of HCC cells. KEGG pathway analysis showed that NEK2 might affected pathways of the cell cycle. Moreover, NEK2-related genes were mainly enriched in the S and G2 phases and might act as tumor-promoting genes by regulating the S/G2 phase transition of HCC cells.

Conclusions
An anti-NEK2 mAb with high potency, high affinity and high specificity was prepared by prokaryotic expression system in this study and may be used in the establishment of ELISA detection kits and targeted treatment of liver cancer.
Narratives: a review of concepts, determinants, effects, and uses in migration research
Authors: Dennison James 
Published: October 2021

AbstractNarratives are increasingly cited by scholars, international organisations, NGOs, and governments as one of the most powerful factors in migration politics and policymaking today. However, narratives are typically conceptually underspecified, with relatively little known about why some narratives become publicly popular or the nature of their effects. This article reviews recent scholarly advances to specify what narratives are and to offer a novel theoretical framework to better explain variation in their public popularity and effects. It is argued that the popularity of a narrative, defined as a generalisable, constructed and selective depiction of reality across time, is determined by a combination of contextual factors, such as issue complexity and salience, the plausibility of the narrative and the traits of the recipient of the narrative. These findings are relevant for policymakers and, particularly, communicators. However, although significant work has gone into explaining how narratives affect migration policymaking, the often-assumed effects of narratives on attitudes to immigration and migration behaviour have rarely been robustly tested.
Antibacterial, antibiofilm, and cytotoxic activities and chemical compositions of Peruvian propolis in an in vitro oral biofilm
Authors: Millones Gómez Pablo Alejandro , Tay Chu Jon Lidia Yileng , Maurtua Torres Dora Jesús , Bacilio Amaranto Reyma Evelyn , Collantes Díaz Ingrit Elida , Minchón Medina Carlos Alberto 
Published: October 2021

Background: Natural products with antibacterial potential have begun to be tested on biofilm models, bringing us closer to understanding the response generated by the complex microbial ecosystems of the oral cavity. The objective of this study was to evaluate the antibacterial, antibiofilm, and cytotoxic activities and chemical compositions of Peruvian propolis in an in vitro biofilm of Streptococcus gordonii and Fusobacterium nucleatum. Methods: The experimental work involved a consecutive, in vitro, longitudinal, and double-blinded study design. Propolis samples were collected from 13 different regions of the Peruvian Andes. The disk diffusion method was used for the antimicrobial susceptibility test. The cytotoxic effect of propolis on human gingival fibroblasts was determined by cell viability method using the MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) assay, and the effect of propolis on the biofilm was evaluated by confocal microscopy and polymerase chain reaction (PCR). Results: The 0.78 mg/mL and 1.563 mg/mL concentrations of the methanolic fraction of the chloroform residue of Oxapampa propolis showed effects on biofilm thickness and the copy numbers of the srtA gene of S. gordonii and the radD gene of F. nucleatum at 48 and 120 hours, and chromatography (UV, λ 280 nm) identified rhamnocitrin, isorhamnetin, apigenin, kaempferol, diosmetin, acacetin, glycerol, and chrysoeriol. Conclusions: Of the 13 propolis evaluated, it was found that only the methanolic fraction of Oxapampa propolis showed antibacterial and antibiofilm effects without causing damage to human gingival fibroblasts. Likewise, when evaluating the chemical composition of this fraction, eight flavonoids were identified.
Targeting MICA/B with cytotoxic therapeutic antibodies leads to tumor control
Authors: Bléry Mathieu , Mrabet-Kraiem Manel , Morel Ariane , Lhospice Florence , Bregeon Delphine , Bonnafous Cécile , Gauthier Laurent , Rossi Benjamin , Remark Romain , Cornen Stéphanie , Anceriz Nadia , Viaud Nicolas , Trichard Sylvia , Carpentier Sabrina , Joulin-Giet Alix , Grondin Gwendoline , Liptakova Veronika , Kim Younghoon , Daniel Laurent , Haffner Aurélie , Macagno Nicolas , Pouyet Laurent , Perrot Ivan , Paturel Carine , Morel Yannis , Steinle Alexander , Romagné François , Narni-Mancinelli Emilie , Vivier Eric 
Published: October 2021

Background: MICA and MICB are tightly regulated stress-induced proteins that trigger the immune system by binding to the activating receptor NKG2D on cytotoxic lymphocytes. MICA and MICB are highly polymorphic molecules with prevalent expression on several types of solid tumors and limited expression in normal/healthy tissues, making them attractive targets for therapeutic intervention. Methods: We have generated a series of anti-MICA and MICB cross-reactive antibodies with the unique feature of binding to the most prevalent isoforms of both these molecules. Results: The anti-MICA and MICB antibody MICAB1, a human IgG1 Fc-engineered monoclonal antibody (mAb), displayed potent antibody-dependent cellular cytotoxicity (ADCC) and antibody-dependent cellular phagocytosis (ADCP) of MICA/B-expressing tumor cells in vitro. However, it showed insufficient efficiency against solid tumors in vivo, which prompted the development of antibody-drug conjugates (ADC). Indeed, optimal tumor control was achieved with MICAB1-ADC format in several solid tumor models, including patient-derived xenografts (PDX) and carcinogen-induced tumors in immunocompetent MICAgen transgenic mice. Conclusions: These data indicate that MICA and MICB are promising targets for cytotoxic immunotherapy.
ВЛИЯНИЕ СВЕТОВОЙ ДЕСИНХРОНИЗАЦИИ НА СТРЕСС-ИНДУЦИРУЮЩИЕ И СТРЕСС-БЛОКИРУЮЩИЕ ФИЗИОЛОГИЧЕСКИЕ МЕХАНИЗМЫ У СТАРЫХ МЫШЕЙ
Authors: Zulfugarova Parvin A. 
Published: October 2021

Цель. Исследование механизмов формирования изменений глюкокортикоидной рецепции (в эксперименте на белых беспородных мышах старого возраста) под влиянием световой десинхронизации.
Материалы и методы исследования. Объектами исследования были белые беспородные мыши (n=246), средний возраст которых составил около 19 месяцев. Световую десинхронизацию моделировали путем изменения режима освещения (комбинация естественного и искусственного освещения) в течение 23 дней. В определённые дни после начала десинхронизации проводили взятие проб крови, в которых с помощью иммуноферментного анализатора «Multiskan GO» определяли уровень плазменного кортизола. Радиоиммунологическим методом выявляли комплексы антиген-антитело, в котором один из иммунореагентов был мечен изотопом. Результаты анализировались для выявления сходства или различий (применяли двухвыборочный тест Стьюдента, с оценкой достоверности различий при p<0,05).
Результаты исследования и обсуждение. Результаты анализа уровня экспрессии глюкокортикоидных рецепторов II типа (относится к стресс-индуцирующим факторам) и уровня экспрессии глюкокортикоидных рецепторов III типа (стресс-блокирующий фактор) в различные сроки после проведения световой десинхронизации указывали на патологическое снижение адаптационных способностей. У старых мышей это выражалось в увеличении количества стресс-индуцирующих факторов и неадекватным уменьшении синтеза стресс-блокирующих факторов. Вероятно, у старых мышей в ответ на стрессовые стимулы происходит ослабление протекторных механизмов их организма. Полученные данные не противоречат общепринятым концепциям, которые толкуют роль глюкокортикоидной системы в системе протекторных механизмов организма. Однако, известно, что помимо глюкокортикоидных и антиглюкокортикоидных механизмов имеются и прочие звенья, которые реагируют при световой десинхронизации (уровень цитокинов, эндогенных опиоидов, серотонина и др.). В этом контексте мы запланировали продолжить исследования с большим количеством животных и наряду с изучением при световой десинхронизации глюкокортикоидной рецепции, также провести комплексный анализ показателей иммунного и нейромедиаторного статуса.
Заключение. Исследования влияния световой десинхронизации на стресс-индуцирующие и стресс-блокирующие физиологические механизмы у старых мышей выявили высокую степень стрессовой реакции у них на этот раздражитель. Эти сведения могут быть очень полезны в медицине и биологии. Вместе с тем, для более надёжных выводов необходимо продолжить исследования на ещё большем количестве животных (в том числе молодого возраста) и изучить наряду с глюкокортикоидами показатели иммунного и нейромедиаторного статуса.
СТРУКТУРА МОРФОЛОГИЧЕСКОЙ ИЗМЕНЧИВОСТИ И ВИТАЛИТЕТНАЯ СТРУКТУРА ЦЕНОПОПУЛЯЦИЙ TULIPA GESNERIANA L. НА ЕРГЕНИНСКОЙ ВОЗВЫШЕННОСТИ
Authors: Lidzhieva Nina Ts. , Ochirova Aleksandra S. , Ovadykova Zhanna V. , Ubushaeva Saglara V. 
Published: October 2021

Обоснование. Изучение жизненности индивидуумов и виталитетной структуры популяций является одним из актуальных подходов в оценке состояния редких видов растений, к которым относится объект исследования.
Цель. Определение структуры морфологической изменчивости и виталитетной структуры ценопопуляций Tulipa gesneriana L. на Ергенинской возвышенности.
Материалы и методы. Исследования проводили в течение двух вегетационных сезонов в трех ценопопуляциях из разных растительных сообществ. Уровень изменчивости 12 морфологических признаков растений оценивали по С.А. Мамаеву. В структуре изменчивости выделяли группы системных индикаторов. Для оценки пластичности признака использовали индекс фитоценотической пластичности, виталитетного спектра популяций – методику Ю.А. Злобина, виталитетной структуры популяции – индекс виталитета, виталитетного типа – критерий Q, степени процветания или депрессивности ценопопуляций – индекс IQ.
Результаты. Большинство изученных признаков растений T. gesneriana в первый год исследования имели большие значения, чем в следующем году, варьируя при этом преимущественно среднем и повышенном уровне. В следующем году уровень варьирования признаков возрос.
Структуру изменчивости морфологических признаков T. gesneriana в первый год исследования слагали группы эколого-биологических и биологических системных индикаторов, в следующем году наряду с данными двумя группами появилась группа экологических системных индикаторов. Оценка жизненности ценопопуляций T. gesneriana выявила, что они относятся к типу «процветающие». Степень процветания ценопопуляций колебалась по годам.
Заключение. Таким образом, ценопопуляции T. gesneriana относились к виталитетному типу «процветающие». Во второй год исследования в ценопопуляциях T. gesneriana, показатели жизненности ухудшались.
Servicios ecosistémicos de provisión suministrados por agroecosistemas de huertos familiares del Estado de México
Authors: Arcos Severo Minerva , Gutiérrez Cedillo Jesús Gastón , Balderas Plata Miguel Ángel , Martínez García Carlos Galdino 
Published: October 2021

Introducción: Los servicios ecosistémicos (SE) de provisión suministrados por los agroecosistemas de huertos familiares (AEHF) se refieren a los beneficios tangibles que las personas obtienen del ecosistema. Aunque los AEHF, han sido ampliamente estudiados, pocos son los trabajos que evidencian su abordaje desde una perspectiva integral, la cual incorpore características biofísicas y económicas al mismo tiempo. Objetivo: Realizar un análisis integral los servicios ecosistémicos de provisión suministrados por agroecosistemas de huertos familiares del Estado de México. Métodos: Se recopilo información sobre los SE de provisión (identificación, usos, destinos y generación de ingresos) mediante entrevistas semiestructuradas, cuestionarios, inventarios de especies, observaciónes directas, y recorridos en campo entre Marzo a Diciembre de 2019 en tres comunidades rurales, con la participación de 42 familias propietarias de los huertos familiares. Resultados: Se identificaron un total de seis SE de provisión asociados a 212 especies, las cuales fueron distribuidas en 172 géneros y 82 familias. Los Principales usos que los propietarios de los huertos dieron a los SE de provisión fueron ornamentales (51 %), seguidos de los alimenticios (39 %) y medicinales (32 %), donde especies como Cymbopogon citratus, Persea americana, Punica granatum, Zea mays e Inga jinicuil, tuvieron los mayores valores de uso (V. U. = 4). El destino de la producción fue autoconsumo, venta e intercambio; siendo el primero, la principal estrategia de sobrevivencia utilizada por los hogares. La venta de productos comestibles en fresco, así como de sus derivados represento ingresos brutos promedio de 1 333.47 USD/año para los propietarios de los huertos familiares. Conclusiones: Los servicios ecosistémicos de provisión proporcionados por los huertos familiares son vistos como una fuente importante de recursos, los cuales permiten satisfacer necesidades múltiples de los hogares rurales, catalogados en pobreza extrema.
The comparative study of invasive electroencephalography modalities in temporal lobe epilepsy
Authors: Dzhafarov V. M. , Dmitriev A. B. , Denisova N. P. , Rzaev D. A. 
Published: October 2021

Introduction. Invasive video-EEG monitoring (invasive EEG) is indicated in patients with refractory focal epilepsy while localization of the epileptogenic zone is unclear. Methods of invasive EEG in different groups of patients demonstrate variable results.Objective: to analyse the results of invasive EEG via subdural and depth electrodes in patients with refractory temporal lobe epilepsy with mesial temporal lobe seizures.Materials and methods. The series of 37 patients who underwent invasive EEG from 2013 to 2020 was retrospectively analysed. The study includes primary adult patients with structural refractory focal epilepsy with mesial temporal lobe seizures without tumor and vascular pathology. Patients were divided onto 3 groups: 1) with foramen ovale electrodes 2) subdural strip electrodes and 3) combination of subdural strips and depths electrodes. The results of anteromedial temporal lobectomy after 6 months were classified according to Engel scale.Results. A group with foramen ovale electrodes included 7 patients, subdural strips – 23, combination – 7. The seizure onset zone was detected in 36 (97 %) cases. Serious complications were observed in 2 (29 %) cases in the group with foramen ovale electrodes. The mean follow-up in 23 (76 %) patients after resective surgery was 28.3 months. Favourable results (Engel I, II) were observed in 4 (80 %) patients with foramen ovale electrodes, in 8 (67 %) patients with subdural electrodes, in 6 (100 %) with combination. Unfavourable results (Engel III, IV) were noted in 1 (20 %) patient with foramen ovale electrode, in 4 (33 %) patients with subdural strips.Conclusion. All the presented modalities of invasive EEG are effective for localizing of seizure onset zone in this category of patients. Foramen ovale electrode using may be limited due to increased risk of complications.