bluuebunny/crossref_metadata_2025_split huggingface dataset:
A new insight into aggregation of oncolytic adenovirus Ad5-delta-24-RGD during CsCl gradient ultracentrifugation
Authors: Stepanenko Aleksei A. , Sosnovtseva Anastasiia O. , Valikhov Marat P. , Chekhonin Vladimir P. 
Published: August 2021

AbstractTwo-cycle cesium chloride (2 × CsCl) gradient ultracentrifugation is a conventional approach for purifying recombinant adenoviruses (rAds) for research purposes (gene therapy, vaccines, and oncolytic vectors). However, rAds containing the RGD-4C peptide in the HI loop of the fiber knob domain tend to aggregate during 2 × CsCl gradient ultracentrifugation resulting in a low infectious titer yield or even purification failure. An iodixanol-based purification method preventing aggregation of the RGD4C-modified rAds has been proposed. However, the reason explaining aggregation of the RGD4C-modified rAds during 2 × CsCl but not iodixanol gradient ultracentrifugation has not been revealed. In the present study, we showed that rAds with the RGD-4C peptide in the HI loop but not at the C-terminus of the fiber knob domain were prone to aggregate during 2 × CsCl but not iodixanol gradient ultracentrifugation. The cysteine residues with free thiol groups after the RGD motif within the inserted RGD-4C peptide were responsible for formation of the interparticle disulfide bonds under atmospheric oxygen and aggregation of Ad5-delta-24-RGD4C-based rAds during 2 × CsCl gradient ultracentrifugation, which could be prevented using iodixanol gradient ultracentrifugation, most likely due to antioxidant properties of iodixanol. A cysteine-to-glycine substitution of the cysteine residues with free thiol groups (RGD-2C2G) prevented aggregation during 2 × CsCl gradient purification but in coxsackie and adenovirus receptor (CAR)-low/negative cancer cell lines of human and rodent origin, this reduced cytolytic efficacy to the levels observed for a fiber non-modified control vector. However, both Ad5-delta-24-RGD4C and Ad5-delta-24-RGD2C2G were equally effective in the murine immunocompetent CT-2A glioma model due to a primary role of antitumor immune responses in the therapeutic efficacy of oncolytic virotherapy.
Clinicopathological characteristics and prognosis of gastrointestinal vascular tumours
Authors: Haibin Zhou , Lingling Wang , Lexing Zhang , Xumin Bao , Yingyu Wang , Jianfeng Yang , Xiaofeng Zhang 
Published: August 2021

AbstractTo evaluate the clinicopathological characteristics and prognosis of gastrointestinal vascular tumours. By reviewing the information from the electronic medical record system and pathology database of Hangzhou First People's Hospital affiliated with Zhejiang University School of Medicine and Jiaxing First People’s Hospital from June 2008 to December 2019, 31 patients pathologically diagnosed with vascular tumours were included in this study. The age of onset, sex differences, clinical manifestations, imaging and endoscopic characteristic manifestations, pathological characteristics, treatment methods and prognosis were analysed. The pathological classification was haemangiolymphangioma, haemangioma, and lymphangioma in 8, 14, and 9 cases, respectively. The age of onset was 44–66 years, with no significant difference according to sex (P = 0.583); 32.26% (10/31) of patients had no noticeable symptoms, 37.5% (12/31) of patients had gastrointestinal bleeding, and 6.45% (2/31) of patients, all with lymphangioma, had intestinal obstruction. The lesions were located in and below the duodenum. Endoscopy showed colour differences. Both endoscopic and surgical treatments were safe and effective. The mean survival time was 57.06 ± 35.64 months. Regarding vascular tumours without typical symptoms, the main pathological classification is haemangioma. Vascular tumours are often clinically identified because of bleeding or obstruction and can be treated with endoscopy or surgery. Clinical follow-up is recommended because no invasive manifestations or instances of recurrence were observed.
Detection of fake-video uploaders on social media using Naive Bayesian model with social cues
Authors: Li Xiaojun , Li Shaochen , Li Jia , Yao Junping , Xiao Xvhao 
Published: August 2021

AbstractWith the rapid development of the Internet, the wide circulation of disinformation has considerably disrupted the search and recognition of information. Despite intensive research devoted to fake text detection, studies on fake short videos that inundate the Internet are rare. Fake videos, because of their quick transmission and broad reach, can increase misunderstanding, impact decision-making, and lead to irrevocable losses. Therefore, it is important to detect fake videos that mislead users on the Internet. Since it is difficult to detect fake videos directly, we probed the detection of fake video uploaders in this study with a vision to provide a basis for the detection of fake videos. Specifically, a dataset consisting of 450 uploaders of videos on diabetes and traditional Chinese medicine was constructed, five features of the fake video uploaders were proposed, and a Naive Bayesian model was built. Through experiments, the optimal feature combination was identified, and the proposed model reached a maximum accuracy of 70.7%.
Social communication activates the circadian gene Tctimeless in Tribolium castaneum
Authors: Rath Animesha , Benita Miriam , Doron Josef , Scharf Inon , Gottlieb Daphna 
Published: August 2021

AbstractChemical communication via pheromones is an integral component in insect behavior, particularly for mate searching and reproduction. Aggregation pheromones, that attract conspecifics of both sexes, are particularly common and have been identified for hundreds of species. These pheromones are among the most ecologically selective pest suppression agents. In this study, we identified an activating effect of the aggregation pheromone of the red flour beetle,Tribolium castaneum(Herbst) (Coleoptera: Tenibroidae) on a highly conserved circadian clock gene (Tctimeless).Tribolium castaneumis one of the most damaging cosmopolitan pest of flour and other stored food products. Its male produced aggregation pheromone, 4,8-dimethyldecanal (DMD), attracts both conspecific males and females and is used for pest management via monitoring and mating disruption. TheTctimelessgene is an essential component for daily expression patterns of the circadian clock and plays vital roles in eclosion, egg production, and embryonic development. In this study, we demonstrate that constant exposure to the species-specific aggregation pheromone led toTctimelessup-regulation and a different pattern of rhythmic locomotive behavior. We propose that changing the well-adapted "alarm clock", using DMD is liable to reduce fitness and can be highly useful for pest management.
Assessing effectiveness of exclusion fences in protecting threatened plants
Authors: Lorite Juan , Salazar-Mendías Carlos , Pawlak Roza , Cañadas Eva María 
Published: August 2021

AbstractOvergrazing stands out as threat factors on biodiversity, being especially harmful in the Mediterranean, due to strong human pressure and an accelerated climate change acting synergistically. Fencing is a common tool used in conservation biology to tackle this problem. Advantages of fences are usually fast, intuitive, and easy to evaluate. However, disadvantages could also arise (increasing interspecific competition, disturbing habitat structure, limiting pollination, reducing dispersion). Together with management issues (maintenance, conflicts with stakeholders, and pulling effect). Effectiveness of fencing for conservation has been frequently assessed for animals, while it is almost a neglected topic in plants. We evaluated the outcome of fencing three threatened and narrow-endemic plants. Selected 5 populations were only partly fenced, which allowed comparing different variables inside and outside the fence. For evaluating the fencing effects, we sampled several habitats (vegetation cover, composition, density of target species), and target-species features (individual size, neighbouring species, and fruit-set). Fencing had strong effects on the habitat and on target-species individuals, showing contrasting responses at species and population level. Particularly, forErodium cazorlanum, fence had a positive effect in one case, and negative in another. InHormathophylla baeticaeffect was positive in all populations. Finally, fencing negatively affectedSolenanthus reverchoniiby increasing competition and limiting seed-dispersal. Fencing outcome was different in assessed species, highlighting the need to a case-by-case evaluation to determine the net balance (pros vs. cons), also its suitability and most favourable option (i.e. permanent vs. temporary fences).
Estrogen mediates sex differences in preoptic neuropeptide and pituitary hormone production in medaka
Authors: Yamashita Junpei , Nishiike Yuji , Fleming Thomas , Kayo Daichi , Okubo Kataaki 
Published: August 2021

AbstractThe preoptic area (POA) is one of the most evolutionarily conserved regions of the vertebrate brain and contains subsets of neuropeptide-expressing neurons. Here we found in the teleost medaka that two neuropeptides belonging to the secretin family, pituitary adenylate cyclase-activating polypeptide (Pacap) and vasoactive intestinal peptide (Vip), exhibit opposite patterns of sexually dimorphic expression in the same population of POA neurons that project to the anterior pituitary: Pacap is male-biased, whereas Vip is female-biased. Estrogen secreted by the ovary in adulthood was found to attenuate Pacap expression and, conversely, stimulate Vip expression in the female POA, thereby establishing and maintaining their opposite sexual dimorphism. Pituitary organ culture experiments demonstrated that both Pacap and Vip can markedly alter the expression of various anterior pituitary hormones. Collectively, these findings show that males and females use alternative preoptic neuropeptides to regulate anterior pituitary hormones as a result of their different estrogen milieu.
Predicting the taxonomic and environmental sources of integron gene cassettes using structural and sequence homology of attC sites
Authors: Ghaly Timothy M. , Tetu Sasha G. , Gillings Michael R. 
Published: August 2021

AbstractIntegrons are bacterial genetic elements that can capture mobile gene cassettes. They are mostly known for their role in the spread of antibiotic resistance cassettes, contributing significantly to the global resistance crisis. These resistance cassettes likely originated from sedentary chromosomal integrons, having subsequently been acquired and disseminated by mobilised integrons. However, their taxonomic and environmental origins are unknown. Here, we use cassette recombination sites (attCs) to predict the origins of those resistance cassettes now spread by mobile integrons. We modelled the structure and sequence homology of 1,978 chromosomal attCs from 11 different taxa. Using these models, we show that at least 27% of resistance cassettes have attCs that are structurally conserved among one of three taxa (Xanthomonadales, Spirochaetes and Vibrionales). Indeed, we found some resistance cassettes still residing in sedentary chromosomal integrons of the predicted taxa. Further, we show that attCs cluster according to host environment rather than host phylogeny, allowing us to assign their likely environmental sources. For example, the majority of β-lactamases and aminoglycoside acetyltransferases, the two most prevalent resistance cassettes, appear to have originated from marine environments. Together, our data represent the first evidence of the taxonomic and environmental origins of resistance cassettes spread by mobile integrons.
High proton conductivity of NaMg1−xLixHx (PO3)3·yH2O with a three-dimensional open framework in the intermediate temperature range
Authors: Ueda Naoya , Nakajima Jun , Mori Daisuke , Taminato Sou , Imanishi Nobuyuki , Higashimoto Shinya , Matsuda Yasuaki 
Published: August 2021

A mixed cation phosphate, NaMg1−xLixHx(PO3)3·yH2O, exhibits a proton conductivity of over 10−2 Scm−1 in the wide temperature range of 150–500 ° C under a non-humidified atmosphere, which is covering the desired operating temperature of fuel cells for automobiles and cogeneration systems.
Cannabidiol modulation of oxidative stress and signalling
Authors: Pereira Sónia R. , Hackett Becky , O’Driscoll David N. , Sun Melody Cui , Downer Eric J. 
Published: August 2021

Abstract
Cannabidiol (CBD), one of the primary non-euphoric components in the Cannabis sativa L. plant, has undergone clinical development over the last number of years as a therapeutic for patients with Lennox-Gastaut syndrome and Dravet syndromes. This phytocannabinoid demonstrates functional and pharmacological diversity, and research data indicate that CBD is a comparable antioxidant to common antioxidants. This review gathers the latest knowledge regarding the impact of CBD on oxidative signalling, with focus on the proclivity of CBD to regulate antioxidants and control the production of reactive oxygen species. CBD is considered an attractive therapeutic agent for neuroimmune disorders, and a body of literature indicates that CBD can regulate redox function at multiple levels, with a range of downstream effects on cells and tissues. However, pro-oxidant capacity of CBD has also been reported, and hence caution must be applied when considering CBD from a therapeutic standpoint. Such pro- and antioxidant functions of CBD may be cell- and model-dependent and may also be influenced by CBD dose, the duration of CBD treatment and the underlying pathology.
Turn the Page, Speech-Language Pathologists: Adequate, Authentic, and Accurate Representation as a Consideration in the Selection of Picture Books for Use in Treatment
Authors: Harris Sierrah , Owen Van Horne Amanda J. 
Published: August 2021

Purpose
This tutorial discusses what it means to be a culturally responsive speech-language pathologist (SLP) and then grounds this discussion in strategies that SLPs can engage in to diversify the books and other materials that they use in clinical practice.

Method
We motivate the tutorial by reviewing policy statements and theoretical information from allied literature. Then, we suggest some ways that SLPs can reflect on their practice to enact an antiracist/culturally responsive approach to treatment, taking the selection of children's literature up as a particular example. We identified strategies that have been suggested across a variety of fields and illustrate these strategies with examples. We both provide recommendations for how to select picture books and also suggest ways to implement these suggestions with accountability.

Conclusions
There is a need for SLPs to reflect on how to be culturally responsive in their practice and to review their materials selection practices with regard to how materials reflect the composition of their caseloads. As a predominantly White profession serving diverse caseloads, we have an ethical obligation to review our choice of materials and align them with culturally responsive practices.
The GRAVITY young stellar object survey
Authors:   GRAVITY Collaboration, Sanchez-Bermudez J. , Caratti o Garatti A. , Garcia Lopez R. , Perraut K. , Labadie L. , Benisty M. , Brandner W. , Dougados C. , Garcia P. J. V. , Henning Th. , Klarmann L. , Amorim A. , Bauböck M. , Berger J. P. , Le Bouquin J. B. , Caselli P. , Clénet Y. , Coudé du Foresto V. , de Zeeuw P. T. , Drescher A. , Duvert G. , Eckart A. , Eisenhauer F. , Filho M. , Gao F. , Gendron E. , Genzel R. , Gillessen S. , Grellmann R. , Heissel G. , Horrobin M. , Hubert Z. , Jiménez-Rosales A. , Jocou L. , Kervella P. , Lacour S. , Lapeyrère V. , Léna P. , Ott T. , Paumard T. , Perrin G. , Pineda J. E. , Rodríguez-Coira G. , Rousset G. , Segura-Cox D. M. , Shangguan J. , Shimizu T. , Stadler J. , Straub O. , Straubmeier C. , Sturm E. , van Dishoeck E. , Vincent F. , von Fellenberg S. D. , Widmann F. , Woillez J. 
Published: August 2021

Context. Protoplanetary disks drive some of the formation process (e.g., accretion, gas dissipation, formation of structures) of stars and planets. Understanding such physical processes is one of the most significant astrophysical questions. HD 163296 is an interesting young stellar object for which infrared and sub-millimeter observations have shown a prominent circumstellar disk with gaps plausibly created by forming planets.
Aims. This study aims to characterize the morphology of the inner disk in HD 163296 with multi-epoch, near-infrared interferometric observations performed with GRAVITY at the Very Large Telescope Interferometer. Our goal is to depict the K-band (λ0 ~ 2.2 μm) structure of the inner rim with milliarcsecond (sub-au) angular resolution. Our data is complemented with archival Precision Integrated-Optics Near-infrared Imaging ExpeRiment (H-band; λ0 ~ 1.65 μm) data of the source.
Methods. We performed a gradient descent parametric model fitting to recover the sub-au morphology of our source.
Results. Our analysis shows the existence of an asymmetry in the disk surrounding the central star of HD 163296. We confirm variability of the disk structure in the inner ~2 mas (0.2 au). While variability of the inner disk structure in this source has been suggested by previous interferometric studies, this is the first time that it is confirmed in the H- and K-bands by using a complete analysis of the closure phases and squared visibilities over several epochs. Because of the separation from the star, position changes, and the persistence of this asymmetric structure on timescales of several years, we argue that it is probably a dusty feature (e.g., a vortex or dust clouds) made by a mixing of silicate and carbon dust and/or refractory grains, inhomogeneously distributed above the mid-plane of the disk.
Rieger-type periodicity in the total irradiance of the Sun as a star during solar cycles 23–24
Authors: Gurgenashvili E. , Zaqarashvili T. V. , Kukhianidze V. , Reiners A. , Oliver R. , Lanza A. F. , Reinhold T. 
Published: August 2021

Context. Total solar irradiance allows for the use of the Sun as a star for studying observations of stellar light curves from recent space missions.
Aims. We aim to study how the mid-range periodicity observed in solar activity indices influences the total solar irradiance.
Methods. We studied periodic variations of total solar irradiance based on SATIRE-S and SOHO/VIRGO data during solar cycles 23–24 on timescales of Rieger-type periodicity. Then we compared the power spectrum of oscillations in the total solar irradiance to those of sunspot and faculae data to determine their contributions.
Results. Wavelet analyses of TSI data reveal strong peaks at 180 days and 115 days in cycle 23, while cycle 24 showed periods of 170 days and 145 days. There are several periods in the sunspot and faculae data that are not seen in total solar irradiance as they probably cancel each other out through simultaneous brightening (in faculae) and darkening (in sunspots). Rieger-type periodicity is probably caused by magneto-Rossby waves in the internal dynamo layer, where the solar cyclic magnetic field is generated. Therefore, the observed periods in the total solar irradiance and the wave dispersion relation allow us to estimate the dynamo magnetic field strength as 10–15 kG.
Conclusions. Total solar irradiance can be used to estimate the magnetic field strength in the dynamo layer. This tool can be of importance in estimating the dynamo magnetic field strength of solar-like stars using light curves obtained by space missions.
Linear response in large deviations theory: a method to compute non-equilibrium distributions
Authors: Freitas Nahuel , Falasco Gianmaria , Esposito Massimiliano 
Published: August 2021

Abstract
We consider thermodynamically consistent autonomous Markov jump processes displaying a macroscopic limit in which the logarithm of the probability distribution is proportional to a scale-independent rate function (i.e. a large deviations principle is satisfied). In order to provide an explicit expression for the probability distribution valid away from equilibrium, we propose a linear response theory performed at the level of the rate function. We show that the first order non-equilibrium contribution to the steady state rate function, g(
x
), satisfies

u

(

x

)

⋅
∇
g

(

x

)

=
−
β

W

˙

(

x

)

where the vector field
u
(
x
) defines the macroscopic deterministic dynamics, and the scalar field

W

˙

(

x

)

equals the rate at which work is performed on the system in a given state
x
. This equation provides a practical way to determine g(
x
), significantly outperforms standard linear response theory applied at the level of the probability distribution, and approximates the rate function surprisingly well in some far-from-equilibrium conditions. The method applies to a wealth of physical and chemical systems, that we exemplify by two analytically tractable models—an electrical circuit and an autocatalytic chemical reaction network—both undergoing a non-equilibrium transition from a monostable phase to a bistable phase. Our approach can be easily generalized to transient probabilities and non-autonomous dynamics. Moreover, its recursive application generates a virtual flow in the probability space which allows to determine the steady state rate function arbitrarily far from equilibrium.
Hydroponically Planting Ipomoea Aquatica Vegetables Using Planting Media from Used Goods
Authors: Nurwahyudi M A , Hatta H 
Published: August 2021

AbstractThis study aims to determine the growth of ipomoea aquatica plants using hydroponic techniques with planting media treatment. Ipomoea aquatica is a plant that is a type of vegetable that is easy to obtain, easy to cultivate, processed consumptive and quite economical. Media for seeding, nursery, used cup as a media for plants, which is used is water to facilitate the growth process. Harvesting is carried out in about 25 days. Maintenance of kangkung plants includes controlling and measuring pH. The research method used is to test the hypothesis using the t-test. From the results of this study, it was found that there was a significant difference in the height of the kangkung plants to the treatment of the planting medias. Uneconomical planting medias / industrial business scale plants have an average height growth of ipomoea aquatica plants which is better and more economical than the use of economical planting medias.
The Potential of Pure Chitosan in Increasing Eggshell Thickness Silver Arabic Chicken (Silver brakel kriel)
Authors: Sahara E , Sandi S , Yosi F , Sari M L , Riswandi  , Agustina R 
Published: August 2021

Abstract
The quality of chicken eggs is greatly influenced by the nutrient ration consumed. Chitosan is a crustacean waste product that is biodegradable and anti-germ so it is suitable to be given to poultry. The purpose of this study was to see the effect of chitosan on the external quality of chicken eggs. The study used a completely randomized design (CRD) with 6 treatments and 5 replications, each replication consisting of 2 Arab chickens aged 4.5 months. The study was conducted for 7 weeks. The treatments given were: R0 (control ration without chitosan), R1 (ration + chitosan 0.5%), R2 (ration + chitosan 1%), R3 (ration + chitosan 1.5%), R4 (ration + chitosan 2%), R5 (ration + chitosan 2.5%). The results showed that chitosan had the potential to improve the external quality of eggs. The dose of chitosan 1.5% in the ration was significantly higher (P <0.05), increasing the thickness of the eggshell of all treatments, while the egg index and egg weight still showed the same value (P> 0.05). The conclusion of this study was that the dose of 1.5% chitosan in the ration had a shell thickness 32.56% higher than the control.