bluuebunny/crossref_metadata_2025_split huggingface dataset:
Multicenter, Randomized, Open-Label, Comparative Study of Therapeutic Efficacy, Safety and Tolerability of BNO 1030 application in the technology of delayed prescription of antibiotics in patients with severe acute tonsillitis
Authors: Popovych V. I. , Koshel I. V. , Malofiichuk O. N. , Pyletska L. I. , Semeniuk O. A. , Martynnyk O. V. , Orlovska R. N. , Leta O. I. 
Published: March 2021

AbstractAcute bacterial tonsillitis occurs in 20 –30 % of immunocompetent children; however, the frequency of antibacterial drug prescriptions reaches up to 90 %. Delayed antibiotic prescription is recommended by current guidelines. The study objective was to determine the efficacy of phytoneering extract BNO 1030 in the technology of delayed antibiotic prescription in patients with severe acute tonsillitis.MethodsIn the multicenter, randomized, open-label, comparative study, 182 out of 200 randomized children with acute tonsillitis aged 6 –12 years completed the study. Evaluation criteria: a reduced severity of sore throat when swallowing and at rest, throat irritation at rest, hyperemia of the tonsils assessed by a physician according to a 4-point scale at each visit compared to Visit 1, dynamics of self-assessment of general well-being, intensity of sore throat and difficulty swallowing according to a 10-point visual analogue scale, frequency of antibiotic prescriptions, therapeutic benefit from BNO 1030 in days.ResultsThe use of phytotherapeutic medicinal product BNO 1030 in addition to the standard symptomatic treatment of severe acute tonsillitis provides a clinically significant, adequate reduction in the symptom severity assessed by a physician at V2 (p < 0.005). There are significant differences in the patient’s self-assessment of the symptoms from treatment Day 2 (p < 0.005). This allows to significantly reduce the duration of systemic antipyretic administration (p < 0.005). In the first days of treatment, when a decision on delay of antibiotic prescription is made, a therapeutic benefit in two days in patients of the treatment group was observed compared to the control group. The use of BNO 1030 in patients with severe acute tonsillitis significantly reduces, by 43.7 % or 2.3 times, the need for prescribing antibiotic therapy as part of the technology of delayed antibiotic prescription (p < 0.005). During treatment, no side effects and complications of the disease were recorded.ConclusionBNO 1030 is a safe and effective medicinal product for the treatment of severe acute tonsillitis in children aged 6 –12 years. It provides a significant therapeutic benefit when administered in addition to standard symptomatic therapy and reduces the irrational antibiotic prescription.Trial registrationClinicalTrials.gov Identifier: NCT04537819https://clinicaltrials.gov/ct2/show/NCT04537819?term=ATi-2&draw=2&rank=1
Environmental impact and life cycle financial cost of hybrid (reusable/ single-use) instruments versus single-use equivalents in laparoscopic cholecystectomy
Authors: Rizan Chantelle , Bhutta Mahmood F 
Published: March 2021

ABSTRACTBackgroundHybrid surgical instruments contain both single-use and reusable components, potentially bringing together advantages from both approaches. The environmental and financial costs of such instruments have not previously been evaluated.MethodsWe used Life Cycle Assessment to evaluate the environmental impact of hybrid laparoscopic clip appliers, scissors and ports used for a laparoscopic cholecystectomy, comparing these with single-use equivalents. We modelled this using SimaPro and ReCiPe midpoint and endpoint methods to determine 18 midpoint environmental impacts including the carbon footprint, and three aggregated endpoint impacts. We also conducted life cycle cost analysis of products, taking into account unit cost, decontamination, and disposal costs.ResultsThe environmental impact of using hybrid instruments for a laparoscopic cholecystectomy was lower than single-use equivalents across 17 midpoint environmental impacts, with mean average reductions of 60%. The carbon footprint of using hybrid versions of all three instruments was around one-quarter of single-use equivalents (1,756 g versus 7,194 g CO2e per operation), and saved an estimated 1.13 e-5 DALYs (disability associated life years, 74% reduction), 2.37 e-8 species.year (loss of local species per year, 76% reduction), and US $ 0.6 in impact on resource depletion (78% reduction). Scenario modelling indicated that environmental performance of hybrid instruments was better even if there was low number of reuses of instruments, decontamination with separate packaging of certain instruments, decontamination using fossil-fuel rich energy sources, or changing carbon intensity of instrument transportation. Total financial cost of using a combination of hybrid laparoscopic instruments was less than half that of single-use equivalents (GBP £131 versus £282).ConclusionAdoption of hybrid laparoscopic instruments could play an important role in meeting carbon reduction targets for surgery, and also save money.
Kinetics and seroprevalence of SARS-CoV-2 antibodies – a comparison of 3 different assays
Authors: Kahre Elisabeth , Galow Lukas , Unrath Manja , Haag Luise , Blankenburg Judith , Dalpke Alexander H. , Lück Christian , Berner Reinhard , Armann Jakob P. 
Published: March 2021

AbstractPurposeComparing seroprevalence and antibody kinetics in three different commercially available assays for SARS-CoV-2.MethodsSerostatus of COVID-19 patients was analyzed 5 months and 10 months after their infection, using three different assays: Diasorin LIAISON®, Euroimmun®, Abbott Diagnostics® ARCHITECT.ResultsSeropositivity at baseline differed significantly depending on the assay (Diasorin 81%, Euroimmun 83%, Abbott 59%). At follow-up antibody levels detected in the Diasorin assay were stable, while there was a significant loss in seropositivity in the Euroimmun and Abbott assays.ConclusionThere are significant differences in SARS-CoV-2 antibody kinetics based on the specific assay used.Trial registration number, date of registrationDRKS00022549, 29.07.2020 “retrospectively registered”
Impact of close interpersonal contact on COVID-19 incidence: evidence from one year of mobile device data
Authors: Crawford Forrest W. , Jones Sydney A. , Cartter Matthew , Dean Samantha G. , Warren Joshua L. , Li Zehang Richard , Barbieri Jacqueline , Campbell Jared , Kenney Patrick , Valleau Thomas , Morozova Olga 
Published: March 2021

AbstractClose contact between people is the primary route for transmission of SARS-CoV-2, the virus that causes coronavirus disease 2019 (COVID-19). We sought to quantify interpersonal contact at the population-level by using anonymized mobile device geolocation data. We computed the frequency of contact (within six feet) between people in Connecticut during February 2020 – January 2021. Then we aggregated counts of contact events by area of residence to obtain an estimate of the total intensity of interpersonal contact experienced by residents of each town for each day. When incorporated into a susceptible-exposed-infective-removed (SEIR) model of COVID-19 transmission, the contact rate accurately predicted COVID-19 cases in Connecticut towns during the timespan. The pattern of contact rate in Connecticut explains the large initial wave of infections during March–April, the subsequent drop in cases during June–August, local outbreaks during August–September, broad statewide resurgence during September–December, and decline in January 2021. Contact rate data can help guide public health messaging campaigns to encourage social distancing and in the allocation of testing resources to detect or prevent emerging local outbreaks more quickly than traditional case investigation.One sentence summaryClose interpersonal contact measured using mobile device location data explains dynamics of COVID-19 transmission in Connecticut during the first year of the pandemic.
Type I, II, and III interferon signatures correspond to COVID-19 disease severity
Authors: Kim Myung-Ho , Salloum Shadi , Wang Jeffrey Y , Wong Lai Ping , Regan James , Lefteri Kristina , Manickas-Hill Zachary ,   MGH COVID-19 Collection & Processing Team, Li Jonathan Z , Sadreyev Ruslan I , Yu Xu G , Chung Raymond T 
Published: March 2021

AbstractWe analyzed the plasma levels of interferons and cytokines, and the expression of interferon-stimulated genes in peripheral blood mononuclear cells in COVID-19 patients with different disease severity. Mild patients exhibited transient type I interferon responses, while ICU patients had prolonged type I interferon responses with hyper-inflammation mediated by interferon regulatory factor 1. Type II interferon responses were compromised in ICU patients. Type III interferon responses were induced in the early phase of SARS-CoV-2 infection, even in convalescent patients. These results highlight the importance of type I and III interferon responses during the early phase of infection in controlling COVID-19 progression.
Lung function changes of divers after single deep heliox diving
Authors: Bao Xiao-Chen , Fang Yi-Qun , Yang Tao , Sun Yong-jun , Ma Jun , Xu Ji , Wang Nan , Wang Fang-Fang 
Published: March 2021

AbstractObjectivesThis study detects the changes in pulmonary function of divers after 80m, 100 m, and 120 m helium-oxygen (heliox) dive. Methods: A total of 26 divers participated in the experiment, of which 15 divers performed the 80m dive, 5 divers performed the 100m dive, and 6 divers performed the 120m dive. The exposure phases included breathing heliox or air in water and O2 in the hyperbaric chamber. Pulmonary function (forced flow-volume) was measured twice before diving, within 30 minutes after diving, and 24 hours after diving. The parameters examined were forced vital capacity (FVC), forced expired volume in 1 second (FEV1), forced expired flow from 25% to 75% volume expired (FEF25-75%), 25-75 percent maximum expiratory flow as compared with vital capacity (MEF 25-75%) and peak expiratory flow (PEF). Results: FEV1/FVC and MEF25% markedly decreased (p = 0.0395, p = 0.0496) within 30min after the 80m dive, but returned to base values at 24h after the dive. Other indicators showed a downward trend within 30min after 80m heliox diving (no statistical difference). Interestingly, FEV1, FEV1/FVC, PEF, MEF decreased after 100m heliox dives, but there was no statistical difference. However, in the 120m heliox dive, FEV1/FVC and MEF75% significantly decreased again after diving (p = 0.0098, p = 0.0073). The relatively small number and more proficient diving skills of divers in 100m and 120m diving may be responsible for the inconsistent results. But when the diving depth reached 120m, results again showed a significant statistical change. Conclusion: Single deep heliox diving can cause temporary expiratory and small airway dysfunction, which can be recovered at 24h after diving.
MRI versus mammography plus ultrasound in women at intermediate breast cancer risk: study design and protocol of the MRIB multicentre randomized controlled trial
Authors: Bonelli Luigina Ada , Calabrese Massimo , Belli Paolo , Corcione Stefano , Losio Claudio , Montemezzi Stefania , Pediconi Federica , Petrillo Antonella , Zuiani Chiara , Camera Lucia , Carbonaro Luca Alessandro , Cozzi Andrea , De Falco Alfano Daniele , Gristina Licia , Panzeri Marta , Poirè Ilaria , Schiaffino Simone , Tosto Simona , Trecate Giovanna , Trimboli Rubina Manuela , Valdora Francesca , Viganò Sara , Sardanelli Francesco 
Published: March 2021

AbstractBackgroundIn women at high/intermediate lifetime risk of breast cancer (BC-LTR), contrast-enhanced magnetic resonance imaging (MRI) added to mammography ±ultrasound (Mx±US) increases sensitivity but decreases specificity. An alternative strategy, MRI alone, potentially more cost-effective, has never been explored. This study aims to assess the characteristics of women who participated in a randomized trial offering MRI alone.MethodsIn this feasibility multicentre randomized controlled trial we compared MRI alone versus Mx+US in women at intermediate BC-risk (allocation ratio 1:1). Eligible women were aged 40 –59, with a 15–30% LTR and/or extremely dense breasts. Two screening rounds per woman were planned in ten centres experienced in MRI screening. Primary endpoint: rate of cancers detected in the two arms after 5 years of follow-up. Secondary endpoints: distribution of the risk profiles among the women enrolled in the trial; distribution of pathological stages and histology of cancers detected; interval cancer rate in the two arms.ResultsFrom 07/2013 to 11/2015, 1,254 women (mean age 47 years) were enrolled: 624 were assigned to Mx+US, 630 to MRI. Most of them were aged below 50 (72%) and premenopausal (45%), 52% used oral contraceptives. Among postmenopausal women, 15% had used hormone replacement therapy. Breast and/or ovarian cancer in mother and/or sisters were reported by 37% of enrolled women, extreme breast density was recorded for 79%, 41% had a 15–30% BC-LTR.ConclusionsThe distribution of BC-risk profile major determinants (breast density and family of breast and ovarian cancer) of enrolled women varied across centres.Trial registrationNCT02210546Key pointsMammography plus ultrasound are commonly used to screen women with dense breastsSupplemental ultrasound increases BC detection rate but also false-positives and potential overdiagnosisWhether increased BC detection by US translates into reduced mortality is unknownMRI alone could be more risk- and cost-effective than mammography plus ultrasound
Insulin-stimulated adiponectin secretion in pregnancy is mediated by inhibition of adiponectin ubiquitination and degradation and is impaired in obesity
Authors: Aye Irving L. M. H. , Rosario Fredrick J. , Kramer Anita , Kristiansen Oddrun , Michelsen Trond M. , Powell Theresa L. , Jansson Thomas 
Published: March 2021

ABSTRACTIn pregnancy, adiponectin serves as an endocrine link between maternal adipose tissue, placental function and fetal growth, with low adiponectin promoting placental function and fetal growth. Circulating adiponectin levels are decreased in obese pregnant women and in gestational diabetes, which is believed to contribute to the insulin resistance and increased risk of fetal overgrowth associated with these conditions. However, the molecular mechanisms governing adiponectin secretion from maternal adipose tissues in pregnancy are poorly understood. Using visceral adipose tissue from lean and obese pregnant mice, we show that obesity in pregnancy is associated with adipose tissue inflammation, ER stress, insulin resistance, increased adiponectin ubiquitination and decreased total abundance of adiponectin. Moreover, adiponectin ubiquitination was increased in visceral fat of obese pregnant women as compared to lean pregnant women. We further observed that insulin prevents, whereas ER stress and inflammation promote, adiponectin ubiquitination and degradation in differentiated 3T3-L1 adipocytes. We have identified key molecular pathways regulating adiponectin secretion in pregnancy. This information will help us better understand the mechanisms controlling maternal insulin resistance and fetal growth in pregnancy and may provide a foundation for the development of strategies aimed at improving adiponectin production in pregnant women with obesity or gestational diabetes.
Telencephalic outputs from the medial entorhinal cortex are copied directly to the hippocampus
Authors: Tsoi Sau Yee , Öncül Merve , Svahn Ella , Robertson Mark , Bogdanowicz Zuzanna , McClure Christina , Sürmeli Gülşen 
Published: March 2021

AbstractComplementary actions of the neocortex and the hippocampus enable encoding and long-term storage of experience dependent memories. Standard models for memory storage assume that sensory signals reach the hippocampus from superficial layers of the entorhinal cortex (EC). Deep layers of the EC on the other hand relay hippocampal outputs to the telencephalic structures including many parts of the neocortex. Here we show that cells in Layer 5a of the medial EC send a copy of their telencephalic outputs back to the CA1 region of the hippocampus. Combining cell-type specific anatomical tracing, high-throughput RNA-sequencing based projection mapping and optogenetics aided circuit mapping, we show that these projections have a unique topography and target hippocampal pyramidal cells and interneurons. Our results suggest that rather than serving as a relay, deep EC may coordinate hippocampal-neocortical interactions in spatial cognition and memory.
Alpha satellite insertion close to an ancestral centromeric region
Authors: Giannuzzi Giuliana , Logsdon Glennis A. , Chatron Nicolas , Miller Danny E. , Reversat Julie , Munson Katherine M. , Hoekzema Kendra , Bonnet-Dupeyron Marie-Noëlle , Rollat-Farnier Pierre-Antoine , Baker Carl A. , Sanlaville Damien , Eichler Evan E. , Schluth-Bolard Caroline , Reymond Alexandre 
Published: March 2021

AbstractHuman centromeres are mainly composed of alpha satellite DNA hierarchically organized as higher-order repeats (HORs). Alpha satellite dynamics is shown by sequence homogenization in centromeric arrays and by its transfer to other centromeric locations, for example during the maturation of new centromeres. We identified during prenatal aneuploidy diagnosis by FISH ade novoinsertion of alpha satellite DNA from the centromere of chromosome 18 (D18Z1) into cytoband 15q26. Although bound by CENP-B, this locus did not acquire centromeric functionality as demonstrated by lack of constriction and absence of CENP-A binding. The insertion was associated with a 2.8 kbp deletion and likely occurred in the paternal germline. The site was enriched in long terminal repeats (LTRs) and located ~10 Mbp from the location where a centromere was ancestrally seeded and became inactive in the common ancestor of humans and apes 20-25 million years ago. Long read mapping to the T2T-CHM13 human genome assembly revealed that the insertion derives from a specific region of chromosome 18 centromeric 12-mer HOR array in which the monomer size follows a regular pattern. The rearrangement did not directly disrupt any gene or predicted regulatory element and did not alter the methylation status of the surrounding region, consistent with the absence of phenotypic consequences in the carrier. This case demonstrates a likely rare but new class of structural variation that we name ‘alpha satellite insertion’. It also expands our knowledge on alphoid DNA dynamics and conveys the possibility that alphoid arrays can relocate near vestigial centromeric sites.
Bidirectional modulation of pain-related behaviors in the zona incerta
Authors: Singh Sudhuman , Valdivia Spring , Soler-Cedeño Omar , Adke Anisha P. , Benowitz Barbara , Velasquez Daniela , Wilson Torri D. , Carrasquillo Yarimar 
Published: March 2021

AbstractCentral amygdala neurons expressing protein kinase C-delta (CeA-PKCδ) are sensitized following nerve injury and promote pain-related responses in mice. The neural circuits underlying modulation of pain-related behaviors by CeA-PKCδ neurons, however, remain unknown. In this study, we identified a functional monosynaptic inhibitory neural circuit that originates in CeA-PKCδ neurons and terminates in the ventral region of the zona incerta (ZI), a subthalamic structure previously linked to pain processing. Behavioral experiments further show that chemogenetic inhibition of GABAergic ZI neurons is sufficient to induce bilateral hypersensitivity in uninjured mice as well as contralateral hypersensitivity after nerve injury. In contrast, chemogenetic activation of GABAergic ZI neurons reverses nerve injury-induced hypersensitivity, demonstrating that silencing of the ZI is required for injury-induced behavioral hypersensitivity. Our results identify a previously unrecognized inhibitory efferent pathway from CeA-PKCδ neurons to the ZI and demonstrate that ZI-GABAergic neurons can bidirectionally modulate pain-related behaviors in mice.
Characterization and Heterotic Grouping of Traditional Assam rice (Oryza sativaL.)
Authors: Kumar Praveen , Sarma Debojit , Hazarika Mainu 
Published: March 2021

AbstractParents of heterotic hybrids are derived from different heterotic groups with high genetic divergence. Classification of traditional Assam rice germplasm in divergent pools will be advantageous to maximize the heterosis and thereby to ensure food security. In the present investigation, a group of 60 upland rice genotypes were characterized using 53 polymorphic simple sequence repeats (SSR) markers out of 83 molecular markers. The genetic divergence study using unweighted Neighbour-joining (UNJ) method clustered the 60 genotypes into 3 major clusters. The eleven most divergent genotypes identified were crossed in half diallel fashion to determine the mid-parent and better-parent heterosis values for the objective of heterotic grouping. No correlation between heterosis and genetic distance can be attributable to the use of a subset of markers not linked to yield or concerned. In genetic distance based heterotic grouping, the intra-group hybrids were recorded a higher frequency of crosses, grain yield per plant, specific combining ability effect, mid parent heterosis, better parent heterosis and standard parent heterosis value than those of inter-group hybrids. Overall, sn extensive choice of parents with attractive traits constellation leading to increased yield of the hybrids for much better complementation must be stressed along with a substantial hereditary distance for augmentation of yield heterosis.