bluuebunny/crossref_metadata_2025_split huggingface dataset:
Empirical prediction of variant-associated cryptic-donors with 87% sensitivity and 95% specificity
Authors: Dawes Ruebena , Joshi Himanshu , Cooper Sandra T. 
Published: July 2021

AbstractPredicting which cryptic-donors may be activated by a genetic variant is notoriously difficult. Through analysis of 5,145 cryptic-donors activated by 4,811 variants (versus 86,963 decoy-donors not used; any GT or GC), we define an empirical method predicting cryptic-donor activation with 87% sensitivity and 95% specificity. Strength (according to four algorithms) and proximity to the authentic-donor appear important determinants of cryptic-donor activation. However, other factors such as auxiliary splicing elements, which are difficult to identify, play an important role and are likely responsible for current prediction inaccuracies. We find that the most frequent mis-splicing events at each exon-intron junction, mined from 40,233 RNA-sequencing samples, predict with remarkable accuracy which cryptic-donor will be activated in rare disease. Aggregate RNA-Sequencing splice-junction data provides an accurate, evidence-based method to predict variant-activated cryptic-donors in genetic disorders, assisting pathology consideration of possible consequences of a variant for the encoded protein and RNA diagnostic testing strategies.
CLARA: A web portal for interactive exploration of the cardiovascular cellular landscape in health and disease
Authors: Dona Malathi S.I. , Hsu Ian , Rathnayake Thushara S. , Farrugia Gabriella E. , Gaynor Taylah L. , Kharbanda Malvika , Skelly Daniel A. , Pinto Alexander R. 
Published: July 2021

ABSTRACTMammalian cardiovascular tissues are comprised of complex and diverse collections of cells. Recent advances in single-cell profiling technologies have accelerated our understanding of tissue cellularity and the molecular networks that orchestrate cardiovascular development, maintain homeostasis, and are disrupted in pathological states. Despite the rapid development and application of these technologies, many cardiac single-cell functional genomics datasets remain inaccessible for most cardiovascular biologists. Access to custom visual representations of the data, including querying changes in cellular phenotypes and interactions in diverse contexts, remains unavailable in publicly accessible data portals. Visualizing data is also challenging for scientists without expertise in processing single-cell genomic data. Here we present CLARA—CardiovascuLAR Atlas—a web portal facilitating exploration of the cardiovascular cellular landscape. Using mouse and human single-cell transcriptomic datasets, CLARA enables scientists unfamiliar with single-cell-omic data analysis approaches to examine gene expression patterns and the cell population dynamics of cardiac cells in a range of contexts. The web-application also enables investigation of intercellular interactions that form the cardiac cellular niche. CLARA is designed for ease-of-use and we anticipate that the portal will aid deeper exploration of cardiovascular cellular landscapes in the context of development, homeostasis and disease. CLARA is freely available at https://clara.baker.edu.au.
Botrytis cinerea BcPTP1 is a late infection phase, cysteine rich protein cytotoxic effector
Authors: Zhu Wenjun , Yu Mengxue , Xu Ran , Bi Kai , Xiong Chao , Liu Zhiguo , Sharon Amir , Jiang Daohong , Wu Mingde , Gu Qiongnan , Gong Ling , Chen Weidong , Wei Wei 
Published: July 2021

AbstractBotrytis cinerea is a broad-host-range necrotrophic phytopathogen responsible for serious crops diseases. To facilitate infection, B. cinerea secretes a large number of effectors that induce plant cell death. In screening secretome data of B. cinerea during infection stage, we identified a phytotoxic protein (BcPTP1) that can also induce immune resistance in plants. BcPTP1 is a small (90 aa), cysteine rich protein without any known domains. Transiently expression of BcPTP1 in leaves caused chlorosis that intensifies with time and eventually lead to cell death. Point mutations in eight of the 10 cysteine residues of BcPTP1 abolished the toxic effect, however residual toxic activity remained after heating the peptide, suggesting contribution of unknown epitopes to protein phytotoxic effect. The transcript level of the bcptp1 gene was low during the first 36 h after inoculation and increased sharply upon transition to the late infection stage, suggesting a role of BcPTP1 in lesion spreading. While statistically insignificant, deletion of the bcptp1 gene led to slightly smaller lesions on bean leaves. Further analyses indicated that BcPTP1 is internalized into plant cells after secreting into the apoplast and its phytotoxic effect is negatively regulated by the receptor-like kinases BAK1 and SOBIR1. Collectively, our findings show that BcPTP1 is a virulence factor that toxifies the host cells and facilitates lesion spreading during the late infection stage.
Unraveling the mechanism to develop health consciousness from organic food: a cross-comparison of Brazilian and Spanish millennials
Authors: Japutra Arnold , Vidal-Branco Murilo , Higueras-Castillo Elena , Molinillo Sebastian 
Published: July 2021

PurposeThe aim of this study is to analyze the drivers of health consciousness related to millennials’ organic food consumption and the impact of health consciousness on millennials’ willingness to pay premium through a cross-cultural study.Design/methodology/approachA comparative analysis was conducted in two countries (Brazil vs Spain). Based on the stimuli-organism-response framework, the authors present a conceptual model to investigate the relationship between cognitive and affective stimuli (i.e. natural content, value for money, sensorial appeal, price fairness, trend, emotional appeal and food safety concern) and customers’ health consciousness with the mediating effect of food safety concern and their impact on the customers’ response (i.e. willingness to pay premium). A survey and a structural equation approach are applied.FindingsThe results show that cognitive and affective stimuli and food safety concern improve millennials’ health consciousness and, consequently, their willingness to pay a premium price for organic food. The results present a high validity correlation of constructs with significant differences between the two countries.Originality/valueThe originality of this study lies in the comparison of drivers of health consciousness and their impact on organic food consumption among millennials from two countries (developed vs developing). This work contributes to the study of organic food consumption with an analysis of the impact of seven drivers on health consciousness and its relationship with willingness to pay premium in a cross-comparison of Brazilian and Spanish millennials.
Research shows role of pre-hire and post-hire outcomes in effectiveness of social media recruitment
Authors: Smith David 
Published: July 2021

Purpose
The authors were motivated to carry out their study by the increasing popularity of social media as a recruitment tool. They focused on the recruitment managers as previous studies have concentrated on the experiences of the candidates.

Design/methodology/approach
The authors wanted to test two hypotheses. The first one was split into two parts – Hypothesis 1A was that: “Recruiters intentions to adopt SMR are significantly related to pre-hire ROs.” And Hypothesis 1B was that: “Recruiters intentions to adopt SMR are significantly related to post-hire ROs.” Their second hypothesis was that: “CS (credibility and satisfaction) will mediate the relationship between SMR intention and ROs”. They surveyed 240 recruitment managers in the manufacturing and services sector, in Gujarat, India.

Findings
The results confirmed both of the hypotheses. Firstly, the responses showed that recruiters intended to use social media as it provides pre-hire benefits. Similarly, results showed SMR is more compatible, less complex and ensures better trialability and observability. Secondly, the research showed SMR intentions are significantly related to post-hire ROs. The study proved that SMR was “cost-effective, attracts better talents and retains them”.

Originality/value
The study suggested social media sites like Facebook could maximize the number of applicants and be more effective than traditional advertising in targeting passive job seekers. Second, it showed managers could optimize their SM post-hire outcomes to improve talent retention. Third, the results suggested that SMR could attract dream candidates by providing credible information. Finally, HR departments needed to understand social media complements rather than replaces traditional recruitment methods.
Effects of air mattress pressure on sleep quality and physiological responses: comparison of shoulder and hip pressure relief
Authors: Baek Yoon Jeong , Kim Seung-Hyun , Kim Sayup , Yoo Eui-Sang , Lee Joo-Young 
Published: July 2021

PurposeThe purpose of the present study was to evaluate the effect of air mattress pressure on sleep quality.Design/methodology/approachTen young healthy males participated in all hard surface [AH], shoulder soft [SS] and shoulder and hip soft mattress [SHS] conditions. The surface pressure for SS and SHS were set at their preferred levels.FindingsThe results showed that sleep efficiencies were over 95% for all the three conditions; there were no significant differences in individual sleep variables among the three conditions, but overall sleep quality was better for SS than AH (p = 0.065); heart rates during sleep was greater for AH than the other two conditions (p < 0.1); and a stronger relationship between clothing and bed microclimate humidity were found for SS and SHS than that for AH.Research limitations/implicationsThese results indicated that the both pressure relief air mattresses that were set at their own preferred levels provided high quality sleep with no marked differences.Practical implicationsAir pressure relief mattresses can improve sleep quality of healthy individuals during sleep at night. The results can be used to understand appropriate pressure distribution on surface mattress according to body region, and also to develop algorithms to provide optimum sleep using mattresses with surface pressure control by body region.Originality/valueThe present study found that the shoulder and/or hip pressure relief air mattresses that were set at their own preferred levels provided high quality sleep with no marked differences.
The transformational leadership role in achieving organizational resilience through adaptive cultures: the case of Dubai service sector
Authors: Madi Odeh Rana B.S. , Obeidat Bader Yousef , Jaradat Mais Osama , Masa'deh Ra'ed , Alshurideh Muhammad Turki 
Published: July 2021

PurposeThis empirical research draws on the existing theory of transformational leadership, adaptive culture and organizational resilience, and investigates the effect of the elected TQM leadership style “transformational leadership” through the mediating effect of adaptive culture on organizational resilience, that is the key of survival during crises like the recent COVID-19 pandemic, which has severely impacted the business globally.Design/methodology/approachThis study exploited a cross-sectional online questionnaire of a random sample of Dubai service firms, with the unit of analysis being at the firm level. In total, 379 usable responses were received. Regression analysis was conducted to test hypotheses.FindingsThe overall findings of this study supported that transformational leadership is positively associated with both adaptive culture and firm's resilience and significantly impacts them. Adaptive culture was found partially mediating the effect of transformational leadership on organizational resilience.Practical implicationsThe research findings provide important insights to practitioners (managers and leaders) to better improve their transformational qualities, as these qualities are expected to improve the organizational adaptive cultures and capacity of resilience.Originality/valueTo the best of the authors' knowledge, this is one of the first studies to examine the transformational leadership effect on organizational adaptive culture and firm's resilience. This investigation expands the boundaries of leadership style theory into new arenas, attempting to partially address the identified knowledge gap in this vein.
Bonded design in the virtual environment: the transition of a participatory design methodology
Authors: Nesset Valerie , Davis Elisabeth C. , Stewart-Robertson Owen , Bible J. Brice 
Published: July 2021

PurposeThis paper examines how bonded design (BD), a participatory design methodology, was influenced by the transition to working in a virtual environment necessitated by the coronavirus disease 2019 (COVID-19) pandemic.Design/methodology/approachAbiding by the participatory design tenets of creativity, learning-by-doing and mutual learning, the BD methodology was created for the specific purpose of fostering meaningful communication and interaction between two disparate groups. Previous iterations of BD are discussed, including its naissance with intergenerational teams, its adaptation to provide a framework for a university-wide initiative, the Faculty Information Technology (IT) Liaison Program that brought together faculty members and IT professional staff, and its current use in helping public librarians to develop with older adults, targeted library programming and services.FindingsAnalysis of the findings from the assessment of the BD methodology in different physical contexts demonstrates that the flexibility in the makeup and order of design techniques (discussion, evaluation, brainstorming, prototyping, consensus-building) makes BD potentially adaptable to online spaces. Recommendations for implementing the BD methodology online are outlined. It is argued that BD’s adaptability makes it an ideal method for creating meaningful and productive collaborations within both physical and virtual environments.Originality/valueThe proposed iteration of the BD methodology responds to a need for innovative practices to foster collaborative work in a virtual environment. BD is a unique, inclusive and cost-effective methodology to encourage meaningful interaction and communication between disparate groups in physical or online contexts.
Do reputational signals matter for nonprofit organizations? An experimental study
Authors: Shahid Subhan , Becker Annika , Kundi Yasir Mansoor 
Published: July 2021

PurposeThis paper aims to untangle the underlying mechanisms through which reputational signals promote stakeholders' intentions to donate in nonprofit organizations via stakeholder trust.Design/methodology/approachThe authors apply a moderated mediation model using an experimental design with N = 248 business and public management students of France.FindingsThe results indicate that both a formal reputational signal (third-party certificate) and an informal reputational signal (self-proclaiming to be social entrepreneurial) affect stakeholder trust and intentions to donate. Stakeholder trust partially mediated the relationship between the formal signal and intentions to donate, and the mediation effect was stronger when an informal signal was present (vs. not present).Practical implicationsTrust is central to the exchange of nonprofit organizations and their external stakeholders. To enhance trust and supportive behavior toward nonprofit organizations, these organizations may consider using formal and informal reputational signaling within their marketing strategies.Originality/valueThis research highlights the pivotal role of formal and informal reputational signals for the enhancing stakeholders' trust and donation behavior in a nonprofit context.
Development and post-dicing wet release of MEMS magnetometer: an approach
Authors: Aditi  , Das Supriyo , Gopal Ram 
Published: July 2021

Purpose
Si-based micro electro mechanical systems (MEMS) magnetometer does not require specialized magnetic materials avoiding magnetic hysteresis, ease in fabrication and low power consumption. It can be fabricated using the same processes used for gyroscope and accelerometer fabrication. The paper reports the dicing mechanism for the released MEMS xylophone magnetic sensor fabricated using wafer bonding technology and its characterization in ambient pressure and under vacuum conditions. The purpose of this paper is to dice the wafer bonded Si-magnetometer in a cost-effective way without the use of laser dicing and test it for Lorentz force transduction.

Design/methodology/approach
A xylophone bar MEMS magnetometer using Lorentz force transduction is developed. The fabricated MEMS-based xylophone bars in literature are approximately 500 µm. The present work shows the released structure (L = 592 µm) fabricated by anodic bonding technique using conducting Si as the structural layer and tested for Lorentz force transduction. The microstructures fabricated at the wafer level are released. Dicing these released structures using conventional diamond blade dicing may damage the structures and reduce the yield. To avoid the problem, positive photoresist S1813 was filled before dicing. The dicing of the wafer, filled with photoresist and later removal of photoresist post dicing, is proposed.

Findings
The devices realized are stiction free and straight. The dynamic measurements are done using laser Doppler vibrometer to verify the released structure and test its functionality for Lorentz force transduction. The magnetic field is applied using a permanent magnet and Helmholtz coil. Two sensors with quality factors 70 and 238 are tested with resonant frequency 112.38 kHz and 114.38 kHz, respectively. The sensor D2, with Q as 238, shows a mechanical sensitivity of 500 pm/Gauss and theoretical Brownian noise-limited resolution of 53 nT/vHz.

Originality/value
The methodology and the study will help develop Lorentz force–based MEMS magnetometers such that stiction-free structures are released using wet etch after the mechanical dicing.
Mechanical and durability properties of adobe blocks filled with date palm wastes
Authors: Zaidi Amine , Izemmouren Ouarda , Taallah Bachir , Guettala Abdelhamid 
Published: July 2021

Purpose
Earthen construction does not meet today’s requirements due to certain limitations such as low water resistance and its high vulnerability to cracking damage. The purpose of this study is to improve the mechanical properties and low durability of adobe blocks by incorporating date palm wastes as a natural reinforcement and lime as a stabilizer.

Design/methodology/approach
Soil from the region of Biskra in Algeria was mixed with sand and lime in suitable ratios. Then, date palm wastes were added to the previous mixture at different ratios (0.3%, 0.6% and 0.9%) by dry mix weight to manufacture adobes. Cubical and cylindrical specimens were prepared and tested in a laboratory to investigate the curing time, mechanical and durability characteristics of the formulated blocks. In addition, X-ray diffraction and scanning electron microscopy (SEM) tests were used to identify the materials.

Findings
It has been observed that the addition of lime to the soil is very beneficial for its stabilization, in particular for an optimum of 12%. The presence of date palm waste in the mixture (soil + lime) generated a significant improvement in tensile strength reaching a rate of about 67%. The same observation was made for the tests of resistance to dry abrasion, resistance to erosion, attack by external sulphate and wetting/drying. However, for cases of compressive strength, water absorption and swelling an unfavorable effect was recorded.

Originality/value
Based on the above-mentioned findings, this paper presents a novel solution to increase the durability of adobe materials using date palm wastes with oven curing at 65°C for about nine days. Adopting such an approach would certainly encourage building durable mud housing on a large scale. This can contribute to solving the acute housing shortage, particularly in poor countries.
Soil acidification mediates changes in soil bacterial community assembly processes in response to agricultural intensification
Authors: Zhou Xingang , Khashi u Rahman Muhammad , Liu Junjie , Wu Fengzhi 
Published: July 2021

SummaryAgricultural intensification is known to alter the assembly of soil microbial communities, which regulate several critical ecosystem processes. However, the underlying ecological processes driving changes in microbial community assembly, particularly at the regional scale, remain poorly understood. Using 16S rDNA sequencing, we characterized soil bacterial community assembly in three land‐use types with increasing land‐use intensity: open fields cultivated with main crops (CF) or vegetables (VF), and greenhouses cultivated with vegetables (VG). Compared with CF, VF and VG altered bacterial community composition and decreased spatial turnover rates of edaphic variables and bacterial communities. Bacterial community assembly was primarily governed by deterministic processes; however, bacterial communities in VF and VG were phylogenetically less clustered and more influenced by variable selection and less by dispersal limitation. Soil pH was the most important edaphic variable mediating the changes in bacterial community assembly processes induced by agricultural intensification. Specifically, decreasing soil pH led to stochastic assembly of bacterial community. Soil pH was lower in more intensively managed lands, especially in case of VG (pH range: 5.86–7.42). Overall, agricultural intensification altered soil bacterial community assembly processes, which was associated with soil acidification. These findings may have implications for improving soil quality and agroecosystem sustainability.
Reactivity of flavanols: Their fate in physical food processing and recent advances in their analysis by depolymerization
Authors: Liu Xuwei , Le Bourvellec Carine , Guyot Sylvain , Renard Catherine M. G. C. 
Published: July 2021

AbstractFlavanols, a subgroup of polyphenols, are secondary metabolites with antioxidant properties naturally produced in various plants (e.g., green tea, cocoa, grapes, and apples); they are a major polyphenol class in human foods and beverages, and have recognized effect on maintaining human health. Therefore, it is necessary to evaluate their changes (i.e., oxidation, polymerization, degradation, and epimerization) during various physical processing (i.e., heating, drying, mechanical shearing, high‐pressure, ultrasound, and radiation) to improve the nutritional value of food products. However, the roles of flavanols, in particular for their polymerized forms, are often underestimated, for a large part because of analytical challenges: they are difficult to extract quantitatively, and their quantification demands chemical reactions. This review examines the existing data on the effects of different physical processing techniques on the content of flavanols and highlights the changes in epimerization and degree of polymerization, as well as some of the latest acidolysis methods for proanthocyanidin characterization and quantification. More and more evidence show that physical processing can affect content but also modify the structure of flavanols by promoting a series of internal reactions. The most important reactivity of flavanols in processing includes oxidative coupling and rearrangements, chain cleavage, structural rearrangements (e.g., polymerization, degradation, and epimerization), and addition to other macromolecules, that is, proteins and polysaccharides. Some acidolysis methods for the analysis of polymeric proanthocyanidins have been updated, which has contributed to complete analysis of proanthocyanidin structures in particular regarding their proportion of A‐type proanthocyanidins and their degree of polymerization in various plants. However, future research is also needed to better extract and characterize high‐polymer proanthocyanidins, whether in their native or modified forms.