bluuebunny/crossref_metadata_2025_split huggingface dataset:
Successful Gas Shutoff Operation Using a Coiled Tubing Telemetry System: A Case History
Authors: Amirov A. , Hadiaman F. , Parra D. , Zeynalov J. , Kok A. 
Published: November 2021

Summary
In a deviated well in the Caspian Sea, the gas/oil ratio (GOR) increased rapidly in 2017. The result was an oil rate decline with several choke backs to manage GOR buildup. After performing two production-logging jobs, it was confirmed that 76% of the gas production was coming from four upper perforations. The main objective was to perform a gas shutoff (GSO) treatment in two stages to reduce gas production by squeezing polymer into the formation and setting packers at a 59° deviation inside a 9⅝-in. casing for temporary isolation of the middle and lower production sands.
Fifteen runs were performed with a tube wire-enabled coiled tubing (CT) telemetry (CTT) system that consists of a customized bottomhole assembly (BHA) that instantaneously transmits differential pressure (DP), temperature, and depth data to the surface through a nonintrusive tube wire installed inside the CT. For the first time in the region, a tension, compression, and torque (TCT) subassembly was deployed to control the entire setting/retrieval process with accurate downhole upward/downward forces. CTT technology was a key element to successfully set two through-tubing inflatable retrievable packers (TTIRPs) by performing casing collar locator correlations at the tubing end, which was 133 and 228 m [measured depth (MD)] shallower from the setting depths. In addition, during the second GSO operation, the GSO gel system crosslink time was modified on the basis of the actual bottomhole temperature (BHT) recorded with the CTT system. Finally, during the third GSO operation, treatment placement was improved, spotting more GSO gel system inside the casing section and avoiding further treatments.
After successful placement of the GSO gel system, a drop from 15.5 to 4.5 MMscf/D in gas production was observed (GOR reduction from 11,000 to 750 MMscf/bbl) with an oil rate increment from 1.4 to 6.04 Mbbl/D. Furthermore, after the gas reduction, the operator was able to produce between 1.5 and 2.0 Mbbl/D from other wells that were choked back on the basis of gas handling capabilities limitations. In the short term, GOR reduction sustained at 3,000 MMscf/bbl and 3.0 Mbb/D oil rate.
The novelty of using the CTT system and TCT subassembly for real-time monitoring of BHA data proved to be beneficial for positioning two TTIRP, modifying GSO gel system design, placing it precisely across target intervals, and retrieving two TTIRPs that in the end provided direct and positive financial impact for the operator.
Numerical Study on Mechanism and Parameters Optimization of Temporary Plugging by Particles in Wellbore
Authors: Zhang Tao , Li Ming , Guo Jianchun , Gou Haoran , Mu Kefan 
Published: November 2021

Summary
The temporary plugging by particles in the wellbore can open new perforation clusters and increase stimulated reservoir volume, but the temporary plugging process of particles is not clear. Therefore, in this paper, we take an ultradeep well in the Tarim Basin as the research object and establish a numerical model based on the coupled computational fluid dynamics-discrete element technology (CFD-DEM) approach, which accurately describes the movement process and mechanism of the temporary plugging particles in the wellbore. Furthermore, the influence of flow rate, concentration of injected particles, and the injected mass ratio of particle size on the temporary plugging effect were studied, respectively. In addition, based on the results of the orthogonal experimental analysis, we obtained the pump rate as the primary factor affecting the effect of temporary plugging, and we recommended the optimal operation parameters for temporary plugging by particles in the field: The pump rate is 2 m3/min, the concentration of the injected temporary plugging particles is 20%, and the ratio of the mass of the injected temporary plugging particles with particle size 1 to 5 mm to the mass of the temporary plugging particles with particle size 5 to 10 mm is 3:1. Finally, a single well that had implemented temporary plugging by particles was used to verify the recommended optimal temporary plugging operation parameters. The research results of this paper provide important guidance and suggestions for the design of temporary plugging schemes on the field.
Impact of Placenta-Derived Mesenchymal Stem Cells Treatment on Patients with Severe Lung Injury Caused by COVID-19 Pneumonia: Clinical and Immunological Aspect
Authors: Chen Mei-Chuan , Lai Kevin Shu-Leung , Chien Ko-Ling , Teng Sing-Teck , Lin Yuh-Rong , Chao Wei , Lee Meng-Jung , Wei Po-Li , Huang Yen-Hua , Kuo Han-Pin , Weng Chih-Ming , Chou Chun-Liang 
Published: November 2021

Abstract
Background:The novel coronavirus disease 2019 (COVID-19) has been a global pandemic health issue since 30, January, 2020. Mortality rate was as high as more than 50% in critically ill patients. The Stem cell treatment is effective in refractory severe critically ill COVID-19 patients, but immune regulation mechanisms have not been reported well. Therefore, we evaluate the clinical efficacy and immune modulation of placenta-derived mesenchymal stem cells (pcMSCs) (MatriPlax) in severe critically ill COVID-19 infection who are refractory to current standard therapies.Methods:Intravenous infusion of 1 × 107 MatriPlax was given to five severe COVID-19 patients at Day 0 and day 4. Serum inflammatory markers and immune profiles were studied at Day 0, 4 and 8. Clinical parameters and 28-days mortality were compared between treated group and control group.Results:The treatment group had no 28-days mortality and Murray’s lung injury score was significantly improved compared with control group. After treatment, Ferritin, C-reactive protein (CRP) and Lactate dehydrogenases (LDH) were significantly reduced and lymphopenia was improved. IL-6, IL-1β, IFN-γ and IL-2 were significantly decreased together with decrease in IL-10 reflecting decreasing intensity of inflammation. Immune cell profiles showed increase in CD4+ T cells (CD4+ naïve T cells, CD4+ memory T cells subtypes), Treg cells, CD19+ B cells (and CD19+ naive B cells, CD27+ switched B cells subtypes) and dendritic cells, and a significant decrease in CD14+ monocytes (and CD16- classical, CD16+ non-classical subtypes) monocytes as well as plasma/plasmablast cells. pc-MSCs treatment suppressed hyper-inflammatory states of innate immune responses to COVID-19 infection by increasing Treg cells, decreasing monocytes and plasma/plasmablast cells, and promoted CD4+ T cells and CD19+ B cells towards adaptive immune responses.Conclusion:The intravenous transplantation of Matriplax was safe and effective for severe critically ill COVID-19 patients, especially those who were refractory to current standard care and immunosuppressive therapies
Temporal Hydrogeochemical Dynamics and Source Apportionment in Groundwater of a Complex Lithology.
Authors: N Ganesh , U Vasudevan , N Devaraj , K Mahalakshmi , Panda Banajarani , Singh Dhiraj Kr 
Published: November 2021

Abstract
The present research reports the level of major ions and other physical parameters like pH, EC and TDS and possible sources of contamination in groundwater from south India. A total of 138 groundwater samples were collected during four different seasons and analyzed for physical parameters and other major ions. Many samples are above or approaching the recommended level of ions for safe drinking water. The groundwater quality has been determined by considering 11 parameters and classified into 5 different categories based upon water quality index (WQI) value. The groundwater of the study area is approaching towards pollution which has to consider for future management of the resource. Different geochemical diagrams like Gibbs and Piper are used to evaluate the process affecting the composition of groundwater. Again, the geostatistical techniques applied to confirm the processes through an integrated approach. Based on result of geochemical investigation, the contamination sources in the groundwater of this region are likely to be from (a) Anthropogenic activities (b) Weathering (c) Agricultural fertilizers. Continuous consumption of such water may pose serious health risk to the residents.
Pathogen Detection and Genotyping With Trace Sample by A Phi29 Based Unbiased Exponential Amplification
Authors: Zhang Xi , Chen Jingjing , Xu Heling , Zhang Qi , Zhang Huan , Han Xiaohu , Chen Zeliang 
Published: November 2021

Abstract
Ticks are vectors for many infectious diseases such as spotted fever group (SFG) rickettsioses and borrelioses. Ticks are valuable material for pathogen ecology study. Ticks have several growth stages with significant varying size, and therefore, in most cases, the collected ticks cannot provide sufficient DNA for subsequent studies, particularly for multiple pathogen screening and genotyping. Unbiased pretreatment of the tick samples for subsequent analysis is an urgent need for subsequent ecological survey and other studies. Phi29 DNA polymerase, an enzyme with strand displacement activity, could exponentially amplify DNA randomly and non-biasedly, generating large quantities of DNA. In the present study, we developed a Phi29 based unbiased exponential amplification (PEA) assay for unbiased treatment of sample nucleic acid to provide sufficient DNA for genetic analysis. By using tick borne pathogen detection and genotype as a model, we tested and evaluated the feasibility of the assay. Nucleic acid were extracted from single ticks and subjected to PEA. The results showed that tick DNA could be amplified up to 10 5 folds. The amplified products were successfully used for pathogen screening and genotyping. With the amplified DNA from single tick, Rickettsia was successfully detected and genotyped. A new genotype of Rickettsia was identified from ticks collected from Dandong city, Liaoning province, Northeast China. This PEA assay is universal and can also be extended to other applications where samples are greatly limited.
A Clinical Nomogram For Predicting Preterm Birth in Women Who Conceived Through Assisted Reproductive Technology
Authors: Lin Yaduan , He Fanchen , Gao Rui , Liu Ting , Zhao Ke , He Jiang , Liu Chaoqun 
Published: November 2021

Abstract
Background: We used prepregnant and gestational characteristics as predictors to develop and validated a nomogram predicting the risk of preterm birth (PTB) in assisted reproductive technology (ART) treated women. Methods: The National Vital Statistics System (NVSS) was queried for singleton ART-treated pregnant women from 2015 to 2019. Multivariable cox regression was used to develop the early (< 32 weeks) or late (< 37 weeks) PTB risk model using both statistical significance and clinical importance criteria for variable selection. The predictive accuracy was assessed, and bootstrapping was used for validation. A nomogram was constructed for the presentation of the final model. Results: ART-treated women who were over 45 years old, black, obese, had a history of cesarean section and PTB, restarting ART within 3 months, prepregnant diabetes, chronic hypertension, gestational diabetes, gestational hypertension, and eclampsia, had the highest risk for late and early-stage PTB. The nomogram with these variables accurately predicted PTB in ART women with a singleton pregnancy. (Brier score:0.121, calibration slope: 0.99, c-index: 0.684). Conclusion: We created a nomogram predicting the risk of early or late PTB in ART women with a singleton pregnancy, which could identify potentially at-risk women who seeking ART treatment and inform appropriate preterm care.
The Relation Between Host TLR9 -1486T/C, rs187084 Gene Polymorphisms and H. Pylori cagA, sodB, hsp60 and vacA Virulence Genes Among Gastric Cancer Patients
Authors: Nabiel Yasmin , Shenouda Ragy , Sultan Ahmad M , Shehata Ahmed , sultan amira M 
Published: November 2021

Abstract
Purpose: To identify different genotypes of TLR9 -1486T/C (rs187084) in patients with gastric cancer and reveal their relation to H. pylori virulence genes (cagA, sodB, hsp60 and vacA). Methods: Patients with gastric cancer were recruited to our study, diagnosed both endoscopically and histo-pathologically. H. pylori strains were isolated from gastric samples by culture and PCR amplification of glmM gene. Virulence genes of H. pylori; cagA, sodB, Hsp60 and vacA, were detected by multiplex PCR. Blood samples were used for genotyping of TLR9 -1486T/C (rs187084) by PCR-RFLP. Results: Out of 132 patients with gastric cancer, 106 (80.3%) were positive for H. pylori by both culture and PCR. Similar number of healthy participants were recruited as controls. The prevalence of virulence genes among the isolated H. pylori were 90.6%, 70.8%, 83.0% and 95.3% for cagA, sodB, hsp60 and vacA respectively. The vacA gene alleles had a prevalence of 95.3% for vacAs1/s2, 52.8% for vacAm1 and 42.5% for vacAm2. The CC genotype of TLR9 -1486T/C had a significantly higher frequency in gastric cancer patients when compared to healthy participants (P = 0.045). Furthermore, the CC genotype demonstrated a significantly higher association with H. pylori strains carrying sodB, hsp60 and vacAm1 virulence genes (P = 0.021, P = 0.049 and P = 0.048 respectively). Conclusions: Patients with CC genotype of TLR9 -1486T/C (rs187084) are at higher risk for development of gastric cancer and its co-existence with H. pylori strains carrying sodB, hsp60 or vacAm1 virulence genes might have a synergistic effect in development of gastric cancer.
Investigating The Prolonged Discharge Process in Pediatric Teaching Hospital of Tehran, Iran: A Cross-Sectional Study
Authors: Vatankhoh Sodabeh , Chopani Azam , Aghdam Emir Tahmazi , Tavakoli Mohammad Esmaeel , Joudyian Nasrin , Nafar Hamideh 
Published: November 2021

Abstract
Background

The patient discharge process is one of the most important factors affecting patient satisfaction, which can affect the quality of services of hospitals, and its prolongation can be detrimental in bed management and result in admission delays for new patients. This study aimed to investigate the factors contributing to the prolongation of the discharge process in Tehran's pediatric teaching hospital.
Methods

The present study was a descriptive cross-sectional study carried out on 300 patients discharged from one of the pediatric teaching hospitals in Tehran in 2019. This study was conducted using a mixed-method and composed of two parts: quantitative and qualitative. Initially, in the quantitative stage, the statistical form was used, the average discharge time in different wards of the hospital was measured using the stopwatch method, and the data were analyzed using SPSS V. 25 software and descriptive statistics. Subsequently, to identify the causes of the prolonged process in some departments and identify practical solutions, a qualitative approach was used, including observations, file reviews, Focus Group Discussion (FGD), brainstorming with experts from the Quality Improvement Office, ward secretaries, clearance unit experts, pharmacy experts, and ward supervisors. Finally, the factors were prioritized and approved using the nominal group method.
Results

The average discharge time was 3 hours and 7 minutes; the shortest discharge time belonged to the blood ward, and the longest discharge time belonged to the kidney ward. The causes of delay in discharge were divided into two categories: organizational and human factors. The most crucial elements were the time required to issue discharge orders, write patient summaries, and maintain patient files.
Conclusion

For improving the quality of the discharge process and patient satisfaction, solutions such as having assistants write file summaries before the morning round, sending files out of the ward on time, hiring a circular secretary to collect patients' files from various wards, and controlling them using a standard checklist, nurses' training, and continuous supervision were proposed.
The Flip-Flop Neuron – A Memory Efficient Alternative for Solving Challenging Sequence Processing and Decision Making Problems
Authors: Kumari Sweta , Vigneswaran C , Chakrava V. Srinivasa 
Published: November 2021

Abstract
Sequential decision making tasks that require information integration over extended durations of time are challenging for several reasons including the problem of vanishing gradients, long training times and significant memory requirements. To this end we propose a neuron model fashioned after the JK flip-flops in digital systems. A flip-flop is a sequential device that can store state information of the previous history. We incorporate the JK flip-flop neuron into several deep network architectures and apply the networks to difficult sequence processing problems. The proposed architectures include flip-flop neural networks (FFNNs), bidirectional flip-flop neural networks (BiFFNNs), convolutional flip-flop neural networks (ConvFFNNs), and bidirectional convolutional flip-flop neural networks (BiConvFFNNs). Learning rules of proposed architectures have also been derived. We have considered the most popular benchmark sequential tasks like signal generation, sentiment analysis, handwriting generation, text generation, video frame prediction, lung volume prediction, and action recognition to evaluate the proposed networks. Finally, we compare the results of our networks with the results from analogous networks with Long Short-Term Memory (LSTM) neurons on the same sequential tasks. Our results show that the JK flip-flop networks outperform the LSTM networks significantly or marginally on all the tasks, with only half of the trainable parameters.
LncRNA PCAT6 as a Predictor of Poor Colorectal Cancer Patient Prognosis: A TCGA Dataset Analysis
Authors: Han Lin , Sun Yanjun , Sun Dengqun 
Published: November 2021

Abstract
Background: The long non-coding RNA (lncRNA)) PCAT6 has been studied in many cancers, yet its relationship with colorectal cancer (CRC) remains poorly defined. Here, we conducted an analysis of The Cancer Genome Atlas (TCGA) database to better clarify the role of PCAT6 in this cancer type.Materials and Methods: Wilcoxon rank-sum tests were utilized to assess relative levels of PCAT6 in CRC tumors and normal tissues, while logistic regression analyses were utilized to compare the relationships between PCAT6 levels and clinicopathological findings. Kaplan-Meier curves and Cox regression analyses were used to gauge correlations between PCAT6 and patient survival outcomes, while the biological roles of this lncRNA were investigated via a gene set enrichment analysis (GSEA) approach.Results: PCAT6 levels were significantly correlated with CRC patient N stage (OR = 1.8 for N1&N2 vs. N0), lymphatic invasion (OR = 1.9 for Yes vs. No), M stage (OR = 2.1 for M1 vs. M0), CEA level (OR = 1.9 for >5 vs. ≤5), perineural invasion (OR = 1.9 for Yes vs. No), pathologic stage (OR = 1.9 for Stage IIIIV vs. Stage I/II), and neoplasm type (OR = 2.1 for rectal adenocarcinoma vs. colon adenocarcinoma) (all P < 0.05). CRC patients expressing higher PCAT6 levels exhibited poorer survival outcomes than those expressing low levels of this lncRNA (P = 0.017), and in univariate analyses, higher PCAT6 levels were linked to worse overall survival (OS) (HR = 1.540; 95% CI: 1.079-2.1997; P = 0.017), with this relationship also being preserved in a multivariate analysis (HR = 6.892; 95% CI: 1.713-27.727, P = 0.007). GSEA revealed high PCAT6 expression to be linked to differential DNA methylation enrichment, with high PCAT6 levels being associated with changes in base excision repair, cellular senescence, G2 M DNA damage checkpoint, chromatin-modifying enzyme, and gene silencing by RNA activity. Conclusions: PCAT6 represents a promising prognostic biomarker of poor CRC patient survival outcomes, with DNA methylation and RNA-mediated gene silencing being potentially promising mechanistic pathways whereby this lncRNA may shape patient outcomes.
Viral Strategies for Targeting Spinal Neuronal Subtypes in Adult Wild-Type Rodents
Authors: Kaur Jaspreet , Berg Rune W. 
Published: November 2021

Abstract
Targeting specific subtypes of interneurons in the spinal cord is primarily restricted to a small group of genetic model animals. Since the development of new transgenic model animals can be expensive and labor intensive, it is often difficult to generalize these findings and verify them in other model organisms, such as the rat, ferret or monkey, that may be more beneficial in certain experimental investigations. Nevertheless, endogenous enhancers and promoters delivered using an adeno-associated virus (AAV) have been successful in providing expression in specific subtypes of neurons in the forebrain of wildtype animals, and therefore may introduce a shortcut. GABAergic interneurons, for instance, have successfully been targeted using the mDlx promotor, which has recently been developed and is now widely used in wild type animals. Here, we test the specificity and efficiency of the mDlx enhancer for robust targeting of inhibitory interneurons in the lumbar spinal cord of wild-type rats. Since this has rarely been done in the spinal cord, we also test the expression and specificity of the CamKIIa and hSynapsin promoters. We found that mDlx does in fact target many neurons that contain an enzyme for catalyzing GABA, the GAD-65, with high specificity and a small fraction of neurons containing an isoform, GAD-67. Expression was also seen in some motor neurons. Viral injections using the CamKIIa enhancer infected in some glutamatergic neurons but also GABAergic neurons, whereas hSynapsin targets almost all the neurons in the lumbar spinal cord.
Association of 12-Month Contraceptive Supply Policy and Months of Oral Contraception Prescribed by Obstetrics and Gynecology Physicians: An Exploratory Cross-Sectional Study
Authors: Fuerst Megan , Schrote Kaitlin , Garg Bharti , Rodriguez Maria 
Published: November 2021

Abstract
Objective

This study sought to determine if there was a difference in the months of oral contraception prescribed by physicians living in U.S. states with a 12-month supply policy compared to physicians in states without a policy.
Methods

We conducted an exploratory descriptive study using a convenience sample of Obstetrics & Gynecology resident physicians (n=275) in the United States. Standard bivariate analyses were used to compare the difference between groups.
Results

Few physicians in both groups (3.8% with a policy and 1.4% without a policy) routinely prescribed a 12-month supply of contraception. The mean coverage prescribed by providers in states with and without a policy was 2.81 and 2.07 months (p<0.05).
Conclusions

The majority of physicians were unaware of 12-month contraceptive supply policies and unable to correctly write a prescription for 12-months of contraception, regardless of whether they lived in a state with a 12-month contraceptive supply policy. Physician education may be needed to effectively implement 12-month contraceptive supply policies.