bluuebunny/crossref_metadata_2025_split huggingface dataset:
Manufacturability and Surface Characterisation of Polymeric Microfluidic Devices for Bio-medical Applications
Authors: Arcot Yugandhar , G.L Samuel , Kong Lingxue 
Published: November 2021

Abstract
Microfluidic devices fabricated through mechanical micromachining techniques have already been reported to be highly economical when compared to other techniques. Direct mechanical machining processes are generally classified as a one-step manufacturing process, having the advantages of rapid prototyping and batch production. Though there are advancements in ultra-precision machining techniques, the real challenge of direct machining polymeric microfluidic channels is the occurrence of poor surface integrity owing to the change in mechanical as well as viscoelastic properties. This forms the key objective of the present research work, where the major emphasis has been given to understand the applicability of micro-milling techniques in fabricating microfluidic devices, especially for bio-applications. In this research, the mechanical micro-milling technique was used to create microscale channels on polymethylmethacrylate (PMMA) and polycarbonate (PC) materials; wherein the process capability was mainly assessed based on the surface characteristics of the micro features. Furthermore, for the quantitative analysis, a comparative study was also performed by measuring the surfaces roughness and surface energy of the microchannels made by various fabrication routes such as hot embossing and lithography. The experimental results indicate that the micro-milling of PMMA is the preferable choice for fabricating microfluidic devices when compared to PC. Also, for showing the manufacturability of the mechanical micromachining technique, microfluidic channels with serpentine channels were machined with a depth and width of 50µm and 200µm respectively. The applicability of the fabricated microfluidic devices was further validated by evaluating the functioning of these devices for blood cell separation at different dilution rates.
Comparison of the Efficacy and Safety of Non-Steroidal Anti-Inflammatory Drugs and Corticosteroid Drugs for Prevention of Cystoid Macular Edema After Cataract Surgery: A Systematic Review and Network Meta-Analysis
Authors: Li Shanshan , Wang Hui-Hui , Zhang Da-Wei 
Published: November 2021

Abstract
Introduction:

To compare the efficacy and safety of non-steroidal anti-inflammatory drugs (NSAID), corticosteroid (CS), and a combination of both drugs to prevent cystoid macular edema (CME) after cataract surgery.
Methods

We searched Pubmed, Cochrane Library, and Embase electronic databases to assess the relevant randomized controlled trials (RCTs) up to 28 April 2021. Network meta-analysis was registered on PROSPERO (CRD42020182520).
Results

Twenty-four RCTs were included in this review. The NSAID and combination of both drugs were significantly reduced the risk of developing CME than CS alone in non-diabetics and mix population. In the ranking profiles, the combination therapy showed a significant advantage over the single drugs and was less likely to develop CME. Diclofenac was the most likely to reduce the odds of developing CME compared with bromfenac and nepafenac. Dexamethasone was the most likely to reduce the odds of developing CME compared with betamethasone and fluorometholone.
Conclusion

NSAID combination with CS has significantly reduced the risk of developing CME postoperatively than the single drug. Diclofenac was superior to bromfenac and nepafenac in preventing CME. Dexamethasone was superior to betamethasone and fluorometholone in preventing CME.
miR-1275 Targets MDK/AKT Signaling to Inhibit Breast Cancer Chemoresistance by Lessening the Properties of Cancer Stem Cells
Authors: Han Xu , Ma Ge , Wang Xinyang , Fu Jingyue , Wang Jingyi , Xu Jin , Li Minghui , Xia Tiansong , Wang Shui 
Published: November 2021

Abstract
Background: Chemoresistance is a major obstacle in the neoadjuvant chemotherapy (NCT) of locally advanced breast cancer (LABC). Identification of miRNAs as prognostic biomarkers may help overcome chemoresistance of breast cancer (BC). The aim of this study was to evaluate the expression level of miR-1275 in plasma samples and the biological functions in the chemoresistance of BC.Methods: The expression levels of miR-1275 in plasma samples and cells were measured by RT-qPCR. The associations between the expression levels of miR-1275 and clinicopathological features were studied. CRISPR/Cas9-mediated gene editing was used to construct miR-1275 knock-out cells. CCK-8, colony formation assays, epirubicin accumulation assay and xenograft tumor models were used to detect the sensitivity of breast cancer cells to epirubicin in vitro and in vivo. Mammosphere formation assay, flow cytometry analysis and western blot analyses were used to evaluate the effects of miR-1275 on cancer stem cells (CSCs) characteristics in BC cells. Dual-luciferase reporter, RNA pulldown, ELISA and IHC were used to verify the relationship between the expression of miR-1275 and Midkine (MDK). Results: We found that miR-1275 was significantly downregulated in plasma from patients resistant to chemotherapy with LABC and in chemoresistant breast cancer cell lines, while patients with low levels of miR-1275 show poor overall survival. Mir-1275 knock-out promoted chemoresistance in breast cancer cells by increasing the properties of cancer stem cells in vitro and in vivo. Mechanistically, we identified that Midkine was determined to be direct downstream protein of miR-1275 which initiated PI3K/Akt signaling in breast cancer cells.Conclusions: We demonstrated that the high expression level of miR-1275 in plasma predicted better response to NCT. The reduction of miR-1275 promoted BC cells chemoresistance by increasing CSC properties via targeting MDK/AKT axis. The potential of miR-1275 as a new prognostic biomarker and therapeutic target of breast cancer patients was identified.
Learning and Visualizing Chronic Latent Representations Using Electronic Health Records
Authors: Chushig-Muzo David , Soguero-Ruiz Cristina , Bohoyo Pablo de Miguel , Mora-Jiménez Inmaculada 
Published: November 2021

Abstract
Background: Nowadays, patients with chronic diseases such as diabetes and hypertension have reached alarming numbers worldwide. These diseases increase the risk of developing acute complications and involve a substantial economic burden and demand for health resources. The widespread adoption of Electronic Health Records (EHRs) is opening great opportunities for supporting decision-making. Nevertheless, data extracted from EHRs are complex (heterogeneous, high-dimensional and usually noisy), hampering the knowledge extraction with conventional approaches. Methods: We propose the use of the Denoising Autoencoder (DAE), a Machine Learning (ML) technique allowing to transform high-dimensional data into latent representations (LRs), thus addressing the main challenges with clinical data. We explore in this work how the combination of LRs with a visualization method can be used to map the patient data in a two-dimensional space, gaining knowledge about the distribution of patients with different chronic conditions. Furthermore, this representation can be also used to characterize the patient's health status evolution, which is of paramount importance in the clinical setting. Results: To obtain clinical LRs, we considered real-world data extracted from EHRs linked to the University Hospital of Fuenlabrada in Spain. Experimental results showed the great potential of DAEs to identify patients with clinical patterns linked to hypertension, diabetes and multimorbidity. The procedure allowed us to find patients with the same main chronic disease but different clinical characteristics. Thus, we identified two kinds of diabetic patients with differences in their drug therapy (insulin and non-insulin dependant), and also a group of women affected by hypertension and gestational diabetes. We also present a proof of concept for mapping the health status evolution of synthetic patients when considering the most significant diagnoses and drugs associated with chronic patients. Conclusions: Our results highlighted the value of ML techniques to extract clinical knowledge, supporting the identification of patients with certain chronic conditions. Furthermore, the patient's health status progression on the two-dimensional space might be used as a tool for clinicians aiming to characterize health conditions and identify their more relevant clinical codes.
Production of Silk Fibroin Membrane for Heavy Metal Removal in Water
Authors: Sudsandee Suntorn , Dokmaingam Pannipha , Mingkhwan Rachaneekorn , Kiangkoo Nuttapohn , Worakhunpiset Suwalee , Boonying Patharawadee , Hu Chien-Chieh , Laohaprapanon Sawanya 
Published: November 2021

Abstract
Thermodynamically driven salts and annealing processes developed the pure silk fibroin (SF) membranes from Bombyx mori cocoons. For more generation, the silk fibroin solution 5 wt% mixed the sodium chloride (NaCl) and polyethylene glycol (PEG). The top surface and cross-section of silk fibroin mixed with NaCl (SF/NaCl) showed pore in the surface membrane and hydrophilic property. The β-sheet content in the SF/NaCl membrane is higher than pure silk fibroin (SF) and the silk fibroin mixed with PEG (SF/PEG) membranes. Then cadmium (Cd), lead (Pb), and mercury (Hg) metals in water filtered pass through only SF/NaCl experimented. The pressure, pH, and temperature were effect by the flow rate efficiency of SF/NaCl membranes. The results showed that the highest % removal of Cd, Pb, and Hg were 45.36%, 61.43%, and 86.87%, respectively. The best condition of absorption capacity was 8.50, 6.42, and 41.14 mg/g for Cd, Pb, and Hg. As a natural biopolymer, SF/NaCl possibly apply for Hg removal for water treatment, its nontoxicity, excellent biocompatibility, and mechanical toughness.
Effect of Bagasse Fiber As the Renewable Environmentally Friendly Material on the Properties of the Waterborne Intumescent Fireproof Coating
Authors: Zhan Wang , Ma Zhongfei , Sun Zhihao , Li Lixia , Gu Zhaozhan , Chen Le , Jiang Juncheng , Chen Zhiquan 
Published: November 2021

Abstract
Bagasse was applied as fire-retardant filler to prepare the waterborne intumescent fireproof coating. The effect of bagasse on the properties of the fireproof coating was investigated by fire protection test, thermogravimetric (TG), scanning electron microscope (SEM), energy dispersive spectrometer (EDS), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), water resistance test and mechanical properties test. The result demonstrated the coating contained 1.5wt.% bagasse performed best in fire protection, thermal stability, oxidation resistance. Moreover, the char layer was dense and continuous. However, the fireproof coating contained 2 wt.% bagasse had excellent performance in water resistance test and mechanical properties test due to its properties of fiber. In addition, the components and chemical structure of char layer were characterized to study the flame retardant mechanism of bagasse in the fireproof coating.
Identification of two QTLs, BPH41 and BPH42, and their respective gene candidates for brown planthopper resistance in rice by a combinatorial approach of QTL mapping and RNA-seq
Authors: Tan Han Qi , Palyam Sreekanth , Gouda Jagadeesha , Kumar Prakash , Chellian Santhosh Kumar 
Published: November 2021

Abstract
The brown planthopper (BPH) is the leading cause of insect damage to rice plants. It has caused profound losses in rice production since the late 1970’s and continues to be a major pest problem. Therefore, there is an urgent need to discover new BPH resistance genes to ensure the successful production of rice. Here, a new BPH resistance source provided by SeedWorks International Pvt. Ltd., SWD10, was used for this purpose. A total of 232 F2 progenies were genotyped with 216 polymorphic markers. Results revealed two dominant QTLs located on the short and long arms of chromosome 4. These QTLs are referred to as BPH41 and BPH42, respectively. BPH resistance mechanism test revealed that antibiosis and antixenosis mechanisms both play a role in BPH resistance conferred by these two QTLs. To further reduce the size of each QTL, 14,368 recombinant lines were screened, and the QTLs were delimited between markers SWRm_01617 and SWRm_01522 for BPH41, and SWRm_01695 and SWRm_00328 for BPH42. Additionally, using RNA-seq data of lines containing the QTLs, we further shortlisted four and three gene candidates for BPH41 and BPH42, respectively. Differential gene expression analysis of lines containing the QTLs suggested that SWD10 BPH resistance is contributed by the plant’s innate immunity and the candidate genes may be part of the rice innate immunity pathway. The newly identified QTLs are currently being utilized for breeding BPH resistant rice varieties and hybrids.
Characterizing Diversity Based on Phenotypes and Molecular Marker Analyses of Purple Yam (Dioscorea Alata) Germplasm in Southern China
Authors: Chen Xin , Sun Jingyu , Zhu Qianglong , Xiao Yao , Zhang Hongyu , Huang Yingjin , Wang Putao , Cao Tianxu , Hu Rui , Xiang Zijin , Shan Nan , ZHOU Qinghong 
Published: November 2021

Abstract
Purple yam (Dioscorea alata L.), which is widely distributed in southern China, is characterized by great purple to red flesh or skin, as well as nutritional and medical values. However, their intraspecific genetic diversity remains unknown, which inhibits the germplasm innovation and variety protection of purple yams. In this study, genetic diversity of 26 landraces from southern China were carried out by using 17 morphological traits and 9 simple sequence repeat (SSR) markers, respectively. Results of phenotypic traits showed that all the accessions were divided into four groups at the euclidean distance of 1.43, and there is high correlation between the taxa and the regional distribution of yam accessions. The group IV includes the most accessions mainly from Jiangxi province, followed by group III, which contained accessions mostly from Fujian province. In addition, the leaf shape, leaf vein color and tuber flesh color, displayed higher Shannon's diversity index (I) and variation coefficient, which could identify the purple yam resources effectively. Based on the SSR markers analysis, the euclidean distance among all germplasms ranged from 0.17 to 0.79, and the 26 landraces were also clearly separated into four clusters at the euclidean distance of 0.49. Clustering results based on SSR markers and phenotypic traits were consistent roughly. Furthermore, population structure revealed the existence of three sub-populations in all accessions. These results demonstrate the genetic diversity and distinctness among the purple yam from southern China and provide a theoretical basis for the identification and utilization of purple yams.
Longitudinal Changes in The Association Between Coffee Intake and Ischaemic Heart Disease From 1990 to 2018: An International Ecological Study
Authors: SHIRAI Yoshiro , IMAI Tomoko , SEZAKI Ayako , MIYAMOTO Keiko , KAWASE Fumiya , ABE Chisato , SANADA Masayo , INDEN Ayaka , KATO Takumi , SUZUKI-SUGIHARA Norie , SHIMOKATA Hiroshi 
Published: November 2021

Abstract
In previous observational studies, the association between coffee consumption and the risk of cardiovascular disease has reversed from positive to negative over time. This long-term international ecological study examined whether the association between coffee intake and the mortality and incidence rates of ischaemic heart disease (IHD) changed between 1990 and 2018. We obtained data on coffee intake per capita (cups per day) and IHD mortality and incidence per 100,000 population, and socioeconomic and lifestyle indicators for each country from various publicly available databases. We integrated and analysed data from 147 countries with populations of ≥1 million. We employed a linear mixed model analysis to assess the interaction among coffee intake, IHD mortality and incidence rates by year. The random effects in the mixed model were the intercept and slope by year for each country. The mean global coffee intake increased (p<0.001), while IHD mortality (p<0.001) and incidence (p=0.073) decreased. In all models, the interaction between coffee intake and year showed a significant inverse association for IHD mortality and incidence rates (p<0.001 for all). The country-level association between coffee intake and IHD mortality and incidence rates was stronger in the negative direction between 1990 and 2018.
The Impacts of Globalization on Human Resource Development Management
Authors: Gunawan Ali , Kurnia Stephen Gregorius , Ghazali Hasan 
Published: November 2021

The terms “Globalization” and “The Digital Era” are familiar words that sound in our ears which mean progress or development. The term globalization is a term that has links to increase interdependence among nations and people around the world through trade, investment, travel, popular culture and other forms of interaction so that the boundaries of a country become biased. Globalization can bring about changes in all areas such as culture, economy, social, politics, ideology and so on. On the one hand, globalization brings with its positive consequences and on the other hand also carries negative sides. The Digital Era supports the process of Globalization to make it easier to accomplish, which makes it all easy to communicate with anyone and anytime, accessing data and information easily and quickly wherever we are especially supported by the tools of modern softwares and hardwares. The Digital Era can also develop the capabilities of human resources must be able to adjust to use it. For human resources who are unable to compete will be replaced with more capable ones. However, currently Human Resources in Indonesia have not been fully encouraged to enter the Digital Era due to the constraints of several factors, such as the availability of infrastructure, the cost of digital is still inadequate when associated with the number of people with the level education and low technological understanding so they are accustomed to do jobs that do not require special skills. Here ther role of all groups, communities and governments, to further improve the skills of human resources so as to be able to improve the economy and welfare.
The Role of Personality as A Trigger For the Satisfaction of Valuable Bag
Authors: Dewanti Retno , Nabila Siti , Simanjuntak Diana , Mohamed Zainal Siti Rohaida 
Published: November 2021

Community empowerment activities through research results. This article is to investigated the influence of Hedonic Value and Utilitarian Value against Customer Satisfaction with moderating Personality has impacted to Behavioral Intentions on valuable bag. This research using Structural Equation Modeling (SEM) with Smart PLS 3.0 software. Data collected directly from respondents who meet the specified characteristics of population by distributing questionnaires. This study uses a sample of respondents who ever buy or use specific brand of bag. The samples used in this study were 100 respondents. The research findings indicate that hedonic and utilitarian values had a direct effect on customer satisfaction and have direct effect on behavioural intentions. In addition, customer satisfaction directly influenced behavioural intentions. The role of personality as the moderate variable in this study were able to amplify between customer satisfaction and perceived value.
Skeletal Muscle Phenotype in Patients Undergoing Long-Term Hemodialysis Awaiting Kidney Transplantation
Authors: Souweine Jean-Sébastien , Gouzi Fares , Badia Éric , Pomies Pascal , Garrigue Valérie , Morena Marion , Hayot Maurice , Mercier Jacques , Ayoub Bronia , Quintrec Moglie Le , Raynaud Fabrice , Cristol Jean-Paul 
Published: November 2021

Background and objectives
Age and comorbidity-related sarcopenia represent a main cause of muscle dysfunction in patients on long-term hemodialysis. However, recent findings suggest muscle abnormalities that are not associated with sarcopenia. The aim of this study was to isolate functional and cellular muscle abnormalities independently of other major confounding factors, including malnutrition, age, comorbidity, or sedentary lifestyle, which are common in patients on maintenance hemodialysis. To overcome these confounding factors, alterations in skeletal muscle were analyzed in highly selected patients on long-term hemodialysis undergoing kidney transplantation.

Design, setting, participants, & measurements
In total, 22 patients on long-term hemodialysis scheduled for kidney transplantation with few comorbidities, but with a long-term uremic milieu exposure, and 22 age, sex, and physical activity level frequency-matched control participants were recruited. We compared biochemical, functional, and molecular characteristics of the skeletal muscle using maximal voluntary force and endurance of the quadriceps, 6-minute walking test, and muscle biopsy of vastus lateralis. For statistical analysis, mean comparison and multiple regression tests were used.

Results
In patients on long-term hemodialysis, muscle endurance was lower, whereas maximal voluntary force was not significantly different. We observed a transition from type I (oxidative) to type II (glycolytic) muscle fibers, and an alteration of mitochondrial structure (swelling) without changes in DNA content, genome replication (peroxisome proliferator activator receptor γ coactivator-1α and mitochondrial transcription factor A), regulation of fusion (mitofusin and optic atrophy 1), or fission (dynamin-related protein 1). Notably, there were autophagosome structures containing glycogen along with mitochondrial debris, with a higher expression of light chain 3 (LC3) protein, indicating phagophore formation. This was associated with a greater conversion of LC3-I to LC3-II and the expression of Gabaralp1 and Bnip3l genes involved in mitophagy.

Conclusions
In this highly selected long-term hemodialysis population, a low oxidative phenotype could be defined by a poor endurance, a fiber-type switch, and an alteration of mitochondria structure, without evidence of sarcopenia. This phenotype could be related to uremia through the activation of autophagy/mitophagy.

Clinical Trial registration numbers:
NCT02794142 and NCT02040363.