bluuebunny/crossref_metadata_2025_split huggingface dataset:
All that attaches to atrial septum is not myxoma: deception is everywhere!
Authors: Arvind Balaji , Gupta Saurabh Kumar , Arava Sudheer 
Published: June 2021

AbstractPreterm neonates – especially those with prolonged duration of intensive care stay – are prone to develop fungal endocarditis. Majority of these children have a stormy course, however, a few may be relatively asymptomatic. Occasionally these vegetations may be large and pedunculated, originating from the atrial septum, mimicking a cardiac myxoma on echocardiography.
Additional costs of lactose-reduced diets: lactose-free dairy product substitutes are a cost-effective alternative for people with lactose intolerance
Authors: Taeger Mareike , Thiele Silke 
Published: June 2021

AbstractObjective:People with lactose intolerance have to limit their consumption of lactose-containing dairy products which are a main source of Ca. In particular, for low-income people it is of interest which alternative diet form rich in Ca leads to the lowest additional costs. This study aims to calculate the additional costs of lactose-reduced diets and to show which of different options represent the most cost-effective alternative within a lactose-reduced diet.Design:Using linear programming, food baskets with different lactose contents were calculated and were compared to a basic model, reflecting a normal diet without a limitation of lactose. By comparing the costs and the composition of the food baskets, recommendations for a lactose-reduced diet were derived.Setting:Germany.Participants:A consumer panel dataset representative for Germany is used for the calculations. Information on prices and nutrients is derived from the 9429 adult households without children, and information on consumed food quantities from the 3046 single households.Results:The minimum additional food costs depend on the severity of lactose intolerance and range from 0·2 % to 6·1 % per month. It was found that the greatest adjustments due to lactose reduction could be observed within the dairy product group. In this group, with a rising lactose limit, normal milk was increasingly replaced by lactose-free milk.Conclusion:It was shown that a lactose-reduced diet is generally associated with higher food costs. When suffering from lactose intolerance, switching to lactose-free milk seems to be the most cost-effective way to cover nutrient requirements.
Nutritional marketing of plant-based meat-analogue products: an exploratory study of front-of-pack and website claims in the USA
Authors: Lacy-Nichols Jennifer , Hattersley Libby , Scrinis Gyorgy 
Published: June 2021

AbstractObjective:To explore how some of the largest food companies involved in producing alternative proteins (AP) use health and nutrition claims to market their products.Design:We identified the largest food manufacturers, meat processors and AP companies selling plant-based AP products in the USA. Using publicly available data, we analysed the voluntary health and nutrition claims made on front-of-pack labels (FOPL) and company webpages. We also analysed company websites for further nutrition and health-related statements about their products or AP more generally. Claim classification was guided by the INFORMAS (International Network for Food and Obesity/Non-Communicable Diseases Research, Monitoring, and Action Support) taxonomy for health-related food labelling.Setting:USA.Participants:Not applicable.Results:1394 health and nutrition-related FOPL claims were identified on 216 products, including 685 nutrition claims and 709 ‘other health-related’ claims. No FOPL health claims were identified. Most nutrient claims were for nutrients associated with meat, with 94 % of products carrying a protein claim and 30 % carrying a cholesterol claim. 74 % of products carried a GMO-free claim, and 63 % carried a plant-based claim. On their websites, some companies expanded on these claims or discussed the health benefits of specific ingredients.Conclusions:Companies involved in this category appear to be using nutritional marketing primarily to position their products in relation to meat. There is a focus on nutrient and ingredient claims, with discussion of processing largely avoided. The findings highlight the challenges companies face in positioning AP products as healthy against the backdrop of debates about ultra-processed foods.
PUBLIC OPINIONS ABOUT PAYING COLLEGE ATHLETES AND ATHLETES PROTESTING DURING THE NATIONAL ANTHEM
Authors: Allison Rachel , Knoester Chris , Ridpath B. David 
Published: June 2021

AbstractDrawing on insights from Critical Race Theory and framing theory, as well as previous research, this study ties together and analyzes public opinions about two racialized and politicized sports-related issues: (1) the financial compensation of college athletes, and (2) athlete protests during the national anthem. Consequently, we highlight racial/ethnic identities, racial attitudes, and political identities as predictors of these public opinions. Data for our analyses come from a nationally representative sample of U.S. adults who were surveyed just prior to the 2016 presidential election. Descriptive results suggest that well over half of U.S. adults opposed having the NCAA pay college athletes; also, about two-thirds of adults viewed athlete protests during the national anthem as unacceptable. Regression results reveal that Black and Latinx adults were more supportive of paying college athletes and athlete protests during the national anthem than White adults. Other people of color were also more likely than White adults to support paying college athletes. Racial attitudes such as a lack of recognition of racial/ethnic inequalities in education and support for Black Lives Matter also shaped public opinions about these issues in expected ways. Finally, we find that political identities were linked to public opinions about these issues even after accounting for racial/ethnic identities and racial attitudes. Overall, this study documents public opinions about these prominent sports-related issues just prior to the 2016 election of a President who particularly racialized and politicized sports issues. Even then, these sports-related issues had been similarly filtered through both a White racial frame that encourages colorblind racism and a counter frame that promotes antiracist activism. Altogether, the present study offers further evidence of how sports provide cultural terrain for individuals to enact and negotiate racialized and politicized views of sports and society and illustrates how these processes were reflected in public opinions in 2016.
Leptin and adiponectin concentrations in infants with low birth weight: relationship with maternal health and postnatal growth
Authors: Santana-Meneses Juliana Fernandez , Suano-Souza Fabíola I. , Franco Maria do Carmo , Fonseca Fernando L. , Strufaldi Maria Wany L. 
Published: June 2021

AbstractHealth in pregnancy and infancy can affect the risk of chronic non-communicable diseases. We aimed to describe leptin and adiponectin concentrations in low birth weight (LBW) infants and identify possible associations with maternal nutritional status, adequacy for gestational age, nutritional recovery, and current dietary intake. A cross-sectional study with LBW infants (9–12 months) including maternal background and pre-pregnancy nutritional condition was performed. From the Infants: anthropometry at birth and current was expressed as z-score (weight: WAZ, length, head circumference), nutritional recovery, dietary intake, leptin, and adiponectin blood concentrations. The mean age of the 54 infants was 10.0 ± 1.5 months, 32 (59.3%) were female, 36 (66.7%) preterm, 23 (42.6%) small for gestational age (SGA), and 25 pregnancies (46.3%) were twin. Almost all (98%) of the infants intake energy and protein above the recommendation, and 47 (87.6%) consumed ultra-processed foods. At the time of the assessment, 8 (14.8%) were overweight and 4 (7.4%) had short stature. SGA infants showed faster weight recovery (WAZ 1.54; 95% CI 1.17, 1.91;p= 0.001), higher leptin’s concentration (3.0 ng/ml (1.7, 3.0) versus 1.6 ng/ml (0.9, 2.6);p= 0.032)), and leptin/adiponectin ratio (0.13 ± 0.08 versus 0.07 ± 0.07;p= 0.018). The pre-gestational BMI was a modifier of the effect of WAZ on leptin levels (p= 0.027) in LBW infants. Higher pre-gestational BMI increased the effect of WAZ variation (birth and current) on leptin levels. Concluding, LBW infants showed early changes in leptin and adiponectin concentrations, influenced by maternal (pre-gestational BMI), intrauterine (gestational age adequacy – SGA), and postnatal weight gain. This combination of factors may increase the risk of NCD for this group of children.
Impact of a Mixed Ocean Layer and the Diurnal Cycle on Convective Aggregation
Authors: Tompkins Adrian M. , Semie Addisu G. 
Published: June 2021

AbstractWe investigate how ocean feedbacks and the diurnal cycle impact convective aggregation using a slab ocean coupled to a cloud resolving model. With a 20 m mixed layer ocean, aggregation occurs after 25 days. Thinner ocean layers slow the onset of clustering, with a 1 m ocean layer needing around 43 days. The delay is due to anomalous solar radiation in clear sky regions, causing a relative surface warming balanced by a matching cooling in cloudy areas. The resulting gradient in surface heat fluxes opposes low level convergence into convecting regions. On aggregation, the convective regions are surrounded by moist, clear sky regions with the hottest SSTs, toward which convection migrates, while a cold SST patch forms under dry suppressed regions due to enhanced latent heat fluxes and longwave radiation. The next experiment allows a diurnal cycle of 2.5°C in the domain mean SST. This causes convective rainfall to shift from a weak morning maximum to a sharper evening peak, reminiscent of undisturbed tropical observations. However, convection reverts to a weak early morning maximum once aggregation starts due to spatially heterogeneous radiative forcing. This implies thin mixed ocean layers are a necessary but non‐sufficient condition for an afternoon maximum of convection; limited spatial water vapor variability is also necessary. The imposition of the mean diurnal cycle has no statistically significant impact on the mean timing of clustering onset.
Recording Fidelity of Relative Paleointensity Characteristics in the North Pacific Ocean
Authors: Gai Congcong , Liu Yanguang , Shi Xuefa , Sun Chunqing , Jiang Xiaodong , Liu Jianxing , Zhong Yi , Liu Qingsong 
Published: June 2021

AbstractNorth Pacific Ocean sediments are important archives of geomagnetic field and paleoenvironmental evolutions, yet late Quaternary sedimentary sequences in the North Pacific Ocean are less explored because of generally low sedimentation rates and the challenge of dating sediments deposited below the calcite compensation depth. Core NP02 sediments from the North Pacific Ocean contain three visible tephra layers, which are identified as the To‐Of, Spfa‐1, and Kt‐3 tephra, respectively. An age model for core NP02 is established based on tephra correlation and one radiocarbon date, which gives a mean sedimentation rate of ∼11.6 cm/kyr. Magnetic analyses suggest the dominance of two remanence carriers; detrital vortex state and biogenic single domain magnetite. Variable depth offsets between the relative paleointensity (RPI) signals carried by the two remanence carriers adds a negligible age error (<0.5 kyr) to the core NP02 RPI record. Pronounced RPI amplitude variations are observed, and the Rockall and Laschamp excursions are identified in the core NP02 RPI record based on the age model. The fidelity of the core NP02 RPI record is further verified by comparison with other RPI stacks and records, and we demonstrate that the RPI variations can assist in chronology refinement.
Methane-dependent selenate reduction by a bacterial consortium
Authors: Shi Ling-Dong , Lv Pan-Long , McIlroy Simon J , Wang Zhen , Dong Xiao-Li , Kouris Angela , Lai Chun-Yu , Tyson Gene W , Strous Marc , Zhao He-Ping 
Published: June 2021

Abstract
Methanotrophic microorganisms play a critical role in controlling the flux of methane from natural sediments into the atmosphere. Methanotrophs have been shown to couple the oxidation of methane to the reduction of diverse electron acceptors (e.g., oxygen, sulfate, nitrate, and metal oxides), either independently or in consortia with other microbial partners. Although several studies have reported the phenomenon of methane oxidation linked to selenate reduction, neither the microorganisms involved nor the underlying trophic interaction has been clearly identified. Here, we provide the first detailed evidence for interspecies electron transfer between bacterial populations in a bioreactor community where the reduction of selenate is linked to methane oxidation. Metagenomic and metaproteomic analyses of the community revealed a novel species of Methylocystis as the most abundant methanotroph, which actively expressed proteins for oxygen-dependent methane oxidation and fermentation pathways, but lacked the genetic potential for selenate reduction. Pseudoxanthomonas, Piscinibacter, and Rhodocyclaceae populations appeared to be responsible for the observed selenate reduction using proteins initially annotated as periplasmic nitrate reductases, with fermentation by-products released by the methanotrophs as electron donors. The ability for the annotated nitrate reductases to reduce selenate was confirmed by gene knockout studies in an isolate of Pseudoxanthomonas. Overall, this study provides novel insights into the metabolic flexibility of the aerobic methanotrophs that likely allows them to thrive across natural oxygen gradients, and highlights the potential role for similar microbial consortia in linking methane and other biogeochemical cycles in environments where oxygen is limited.
The long non-coding RNA βFaar regulates islet β-cell function and survival during obesity in mice
Authors: Zhang Fangfang , Yang Yue , Chen Xi , Liu Yue , Hu Qianxing , Huang Bin , Liu Yuhong , Pan Yi , Zhang Yanfeng , Liu Dechen , Liang Rui , Li Guoqing , Wei Qiong , Li Ling , Jin Liang 
Published: June 2021

AbstractDespite obesity being a predisposing factor for pancreatic β-cell dysfunction and loss, the mechanisms underlying its negative effect on insulin-secreting cells remain poorly understood. In this study, we identify an islet-enriched long non-coding RNA (lncRNA), which we name β-cell function and apoptosis regulator (βFaar). βFaar is dramatically downregulated in the islets of the obese mice, and a low level of βFaar is necessary for the development of obesity-associated β-cell dysfunction and apoptosis. Mechanistically, βFaar promote the synthesis and secretion of insulin by upregulating islet-specific genes Ins2, NeuroD1, and Creb1 through sponging miR-138-5p. In addition, using quantitative mass spectrometry, we identify TRAF3IP2 and SMURF1 as interacting proteins that are specifically associated with βFaar. We demonstrate that SMURF1 ubiquitin ligase activity is essential for TRAF3IP2 ubiquitination and activation of NF-κB-mediate β-cell apoptosis. Our experiments provide direct evidence that dysregulated βFaar contributes to the development of obesity-induced β-cell injury and apoptosis.
Potent inhibitors of toxic alpha-synuclein identified via cellular time-resolved FRET biosensors
Authors: Braun Anthony R. , Liao Elly E. , Horvath Mian , Kalra Prakriti , Acosta Karen , Young Malaney C. , Kochen Noah Nathan , Lo Chih Hung , Brown Roland , Evans Michael D. , Pomerantz William C. K. , Rhoades Elizabeth , Luk Kelvin , Cornea Razvan L. , Thomas David D. , Sachs Jonathan N. 
Published: June 2021

AbstractWe have developed a high-throughput drug discovery platform, measuring fluorescence resonance energy transfer (FRET) with fluorescent alpha-synuclein (αSN) biosensors, to detect spontaneous pre-fibrillar oligomers in living cells. Our two αSN FRET biosensors provide complementary insight into αSN oligomerization and conformation in order to improve the success of drug discovery campaigns for the treatment of Parkinson’s disease. We measure FRET by fluorescence lifetime, rather than traditional fluorescence intensity, providing a structural readout with greater resolution and precision. This facilitates identification of compounds that cause subtle but significant conformational changes in the ensemble of oligomeric states that are easily missed using intensity-based FRET. We screened a 1280-compound small-molecule library and identified 21 compounds that changed the lifetime by >5 SD. Two of these compounds have nanomolar potency in protecting SH-SY5Y cells from αSN-induced death, providing a nearly tenfold improvement over known inhibitors. We tested the efficacy of several compounds in a primary mouse neuron assay of αSN pathology (phosphorylation of mouse αSN pre-formed fibrils) and show rescue of pathology for two of them. These hits were further characterized with biophysical and biochemical assays to explore potential mechanisms of action. In vitro αSN oligomerization, single-molecule FRET, and protein-observed fluorine NMR experiments demonstrate that these compounds modulate αSN oligomers but not monomers. Subsequent aggregation assays further show that these compounds also deter or block αSN fibril assembly.
Overexpression of differentially expressed AhCytb6 gene during plant-microbe interaction improves tolerance to N2 deficit and salt stress in transgenic tobacco
Authors: Alexander Ankita , Singh Vijay K. , Mishra Avinash 
Published: June 2021

AbstractStenotrophomonas maltophilia has plant growth-promoting potential, and interaction with Arachis hypogaea changes host-plant physiology, biochemistry, and metabolomics, which provides tolerance under the N2 starvation conditions. About 226 suppression subtractive hybridization clones were obtained from plant-microbe interaction, of which, about 62% of gene sequences were uncharacterized, whereas 23% of sequences were involved in photosynthesis. An uncharacterized SSH clone, SM409 (full-length sequence showed resemblance with Cytb6), showed about 4-fold upregulation during the interaction was transformed to tobacco for functional validation. Overexpression of the AhCytb6 gene enhanced the seed germination efficiency and plant growth under N2 deficit and salt stress conditions compared to wild-type and vector control plants. Results confirmed that transgenic lines maintained high photosynthesis and protected plants from reactive oxygen species buildup during stress conditions. Microarray-based whole-transcript expression of host plants showed that out of 272,410 genes, 8704 and 24,409 genes were significantly (p < 0.05) differentially expressed (> 2 up or down-regulated) under N2 starvation and salt stress conditions, respectively. The differentially expressed genes belonged to different regulatory pathways. Overall, results suggested that overexpression of AhCytb6 regulates the expression of various genes to enhance plant growth under N2 deficit and abiotic stress conditions by modulating plant physiology.
Influence of competition and intraguild predation between two candidate biocontrol parasitoids on their potential impact against Harrisia cactus mealybug, Hypogeococcus sp. (Hemiptera: Pseudococcidae)
Authors: Aguirre María B. , Bruzzone Octavio A. , Triapitsyn Serguei V. , Diaz-Soltero Hilda , Hight Stephen D. , Logarzo Guillermo A. 
Published: June 2021

AbstractWhen two or more parasitoid species, particularly candidates for biocontrol, share the same target in the same temporal window, a complex of behaviors can occur among them. We studied the type of interactions (competition and intraguild predation) that existed between the nymphal parasitoids Anagyrus cachamai and A. lapachosus (Hymenoptera: Encyrtidae), two candidate neoclassical biocontrol agents against the Puerto Rican cactus pest mealybug, Hypogeococcus sp. (Hemiptera: Pseudococcidae). The surrogate native congener host in Argentina, the cactus mealybug Hypogeococcus sp., was studied to predict which species should be released; in the case that both should be released, in which order, and their potential impact on host suppression. In the laboratory we conducted experiments where different densities of the host mealybug were exposed to naive females of A. cachamai and A. lapachosus sequentially in both directions. Experiments were analyzed by combining a series of competitive behavioral and functional response models. A fully Bayesian approach was used to select the best explaining models and calculate their parameters. Intraguild predation existed between A. cachamai, the species that had the greatest ability to exploit the resource, and A. lapachosus, the strongest species in the interference competition. The role that intraguild predation played in suppression of Hypogeococcus sp. indicated that a multiple release strategy for the two biocontrol agents would produce better control than a single release; as for the release order, A. lapachosus should be released first.
A control framework to optimize public health policies in the course of the COVID-19 pandemic
Authors: Pataro Igor M. L. , Oliveira Juliane F. , Morato Marcelo M. , Amad Alan A. S. , Ramos Pablo I. P. , Pereira Felipe A. C. , Silva Mateus S. , Jorge Daniel C. P. , Andrade Roberto F. S. , Barreto Mauricio L. , Costa Marcus Americano da 
Published: June 2021

AbstractThe SARS-CoV-2 pandemic triggered substantial economic and social disruptions. Mitigation policies varied across countries based on resources, political conditions, and human behavior. In the absence of widespread vaccination able to induce herd immunity, strategies to coexist with the virus while minimizing risks of surges are paramount, which should work in parallel with reopening societies. To support these strategies, we present a predictive control system coupled with a nonlinear model able to optimize the level of policies to stop epidemic growth. We applied this system to study the unfolding of COVID-19 in Bahia, Brazil, also assessing the effects of varying population compliance. We show the importance of finely tuning the levels of enforced measures to achieve SARS-CoV-2 containment, with periodic interventions emerging as an optimal control strategy in the long-term.
The use of N-acetylcysteine in the prevention of hangover: a randomized trial
Authors: Coppersmith Veronica , Hudgins Sarah , Stoltzfus Jill , Stankewicz Holly 
Published: June 2021

AbstractHangovers resulting from alcohol intoxication can lead to adverse effects ranging from generalized discomfort and work-related absenteeism to emergency department visits from patients seeking symptomatic care. The purpose of this study was to evaluate the efficacy of a low dose (600–1800 mg) of N-Acetylcysteine (NAC) vs placebo on mitigating hangover symptoms. This was a randomized, double-blinded, placebo controlled crossover study involving 49 volunteers who consumed beer to obtain a breath alcohol content (BrAC) of 0.1 g/210L. The participants met on two separate occasions at which time they were given either NAC or placebo capsules. Opposing treatments were administered during the second encounter. The morning after the participant’s intoxication and treatment, a Hangover Symptom Scale Questionnaire was administered to determine subjective changes in hangover symptoms. Data was analyzed by self-control, comparing the participant’s hangover symptom severity when using NAC compared to placebo. No significant difference was found in the general distribution of total hangover scores (P = .45) (NAC = 10; Placebo = 13). There was also no significant difference found in the general distribution of specific hangover symptoms. However, a significant difference was found in the general distribution of total hangover difference scores based on gender (P = .04) (Female − 3.5; Male 2), specifically for nausea (P = .05) and weakness (P = .03). Although no difference was found in the general hangover scale scores, the study was suggestive of gender specific susceptibility with female participants having improved hangover symptoms after NAC use.