bluuebunny/crossref_metadata_2025_split huggingface dataset:
Infant Mortality and the Repeal of Federal Prohibition
Authors: Jacks David S , Pendakur Krishna , Shigeoka Hitoshi 
Published: February 2021

Abstract
Using new data on county-level variation in alcohol prohibition from 1933 to 1939, we investigate whether the repeal of federal prohibition increased infant mortality, both in counties and states that repealed and in neighbouring counties. We find that repeal is associated with a 4.0% increase in infant mortality rates in counties that chose wet status via local option elections or state-wide legislation and with a 4.7% increase in neighbouring dry counties, suggesting a large role for cross-border policy externalities. These estimates imply that roughly twenty-seven thousand excess infant deaths could be attributed to the repeal of federal prohibition in this period.
The novel cystatin C, lactate, interleukin-6, and N-terminal pro-B-type natriuretic peptide (CLIP)-based mortality risk score in cardiogenic shock after acute myocardial infarction
Authors: Ceglarek Uta , Schellong Paul , Rosolowski Maciej , Scholz Markus , Willenberg Anja , Kratzsch Jürgen , Zeymer Uwe , Fuernau Georg , de Waha-Thiele Suzanne , Büttner Petra , Jobs Alexander , Freund Anne , Desch Steffen , Feistritzer Hans-Josef , Isermann Berend , Thiery Joachim , Pöss Janine , Thiele Holger 
Published: February 2021

AbstractBackground Cardiogenic shock (CS) complicating acute myocardial infarction (AMI) still reaches excessively high mortality rates. This analysis is aimed to develop a new easily applicable biomarker-based risk score.Methods and results A biomarker-based risk score for 30-day mortality was developed from 458 patients with CS complicating AMI included in the randomized CULPRIT-SHOCK trial. The selection of relevant predictors and the coefficient estimation for the prognostic model were performed by a penalized multivariate logistic regression analysis. Validation was performed internally, internally externally as well as externally in 163 patients with CS included in the randomized IABP-SHOCK II trial. Blood samples were obtained at randomization. The two trials are registered with ClinicalTrials.gov (NCT01927549 and NCT00491036), are closed to new participants, and follow-up is completed. Out of 58 candidate variables, the four strongest predictors for 30-day mortality were included in the CLIP score (cystatin C, lactate, interleukin-6, and N-terminal pro-B-type natriuretic peptide). The score was well calibrated and yielded high c-statistics of 0.82 [95% confidence interval (CI) 0.78–0.86] in internal validation, 0.82 (95% CI 0.75–0.89) in internal-external (temporal) validation, and 0.73 (95% CI 0.65–0.81) in external validation. Notably, it outperformed the Simplified Acute Physiology Score II and IABP-SHOCK II risk score in prognostication (0.83 vs 0.62; P &lt; 0.001 and 0.83 vs. 0.76; P = 0.03, respectively).Conclusions A biomarker-only score for 30-day mortality risk stratification in infarct-related CS was developed, extensively validated and calibrated in a prospective cohort of contemporary patients with CS after AMI. The CLIP score outperformed other clinical scores and may be useful as an early decision tool in CS.
Insights into the structure and role of seed-borne bacteriome during maize germination
Authors: Figueiredo dos Santos Lidiane , Fernandes Souta Julie , de Paula Soares Cleiton , Oliveira da Rocha Letícia , Luiza Carvalho Santos Maria , Grativol Clicia , Fernando Wurdig Roesch Luiz , Lopes Olivares Fabio 
Published: February 2021

ABSTRACT
Seed germination events modulate microbial community composition, which ultimately influences seed-to-seedling growth performance. Here, we evaluate the germinated maize (variety SHS 5050) root bacterial community of disinfected seed (DS) and non-disinfected seed (NDS). Using a gnotobiotic system, sodium hypochlorite (1.25%; 30 min)-treated seeds showed a reduction of bacterial population size and an apparent increase of bacterial community diversity associated with a significant selective reduction of Burkholderia-related sequences. The shift in the bacterial community composition in DS negatively affects germination speed, seedling growth and reserve mobilization rates compared with NDS. A synthetic bacterial community (syncom) formed by 12 isolates (9 Burkholderia spp., 2 Bacillus spp., and 1 Staphylococcus sp.) obtained from natural microbiota maize seeds herein was capable of recovering germination and seedling growth when reintroduced in DS. Overall, results showed that changes in bacterial community composition and selective reduction of Burkholderia-related members' dominance interfere with germination events and the initial growth of the maize. By cultivation-dependent and -independent approaches, we deciphered seed–maize microbiome structure, bacterial niches location and bacterial taxa with relevant roles in seedling growth performance. A causal relationship between seed microbial community succession and germination performance opens opportunities in seed technologies to build-up microbial communities to boost plant growth and health.
A Chromosome-Painting-Based Pipeline to Infer Local Ancestry under Limited Source Availability
Authors: Molinaro Ludovica , Marnetto Davide , Mondal Mayukh , Ongaro Linda , Yelmen Burak , Lawson Daniel John , Montinaro Francesco , Pagani Luca 
Published: February 2021

Abstract
Contemporary individuals are the combination of genetic fragments inherited from ancestors belonging to multiple populations, as the result of migration and admixture. Isolating and characterizing these layers are crucial to the understanding of the genetic history of a given population. Ancestry deconvolution approaches make use of a large amount of source individuals, therefore constraining the performance of Local Ancestry Inferences when only few genomes are available from a given population. Here we present WINC, a local ancestry framework derived from the combination of ChromoPainter and NNLS approaches, as a method to retrieve local genetic assignments when only a few reference individuals are available. The framework is aided by a score assignment based on source differentiation to maximize the amount of sequences retrieved and is capable of retrieving accurate ancestry assignments when only two individuals for source populations are used.
Development and Initial Validation of the Adolescents’ Ageism Toward Older Adults Scale
Authors: Marchetti Anna , Lommi Marzia , Barbaranelli Claudio , Piredda Michela , De Marinis Maria Grazia , Matarese Maria 
Published: February 2021

AbstractBackground and ObjectivesAgeism toward older adults, prevalent in contemporary societies, seems to be internalized during childhood and consolidated during adolescence. Although several instruments have been developed to measure adolescents’ ageism, they present a number of limitations. The study aimed at developing a new instrument, the Adolescents’ Ageism Toward Older Adults Scale, and testing its psychometric properties.Research Design and MethodsA three-phase process was followed: The instrument’s items were developed empirically from focus groups of adolescents; its content validity was evaluated; and finally, its psychometric properties were tested through a multicenter cross-sectional study involving 575 adolescents.ResultsExploratory factor analysis of the new scale retained 33 items grouped into 6 factors: moodiness, traditionalism, physical deterioration, antitechnologism, sageness, and sociability. Confirmatory factor analysis evidenced a second-order factor structure. Omega coefficients measuring internal consistency were above the cutoff of 0.60 for the 6 factors except for antitechnologism. Intraclass correlation coefficients for the entire scale and for the 6 factors were greater than 0.70, again excepting antitechnologism.Discussion and ImplicationsThe Adolescents’ Ageism Toward Older Adults Scale is a promising measure of adolescents’ negative and positive attitudes toward older adults. Its validation highlighted some criticalities that can be resolved by a few modifications. Further testing of the scale should be conducted after these modifications.
Students’ experience and adherence to containment measures during COVID-19 in Switzerland
Authors: Zysset Annina E , Schlatter Nadine , von Wyl Agnes , Huber Marion , Volken Thomas , Dratva Julia 
Published: February 2021

Summary

Background
Young adults are not considered a risk group, but the public health response to COVID-19 impacts all citizens. We investigated the impact on young adults’ and their adherence to containment measures addressing potential gender differences.

Methods
In April 2020 12 341 students of the Zurich University of Applied Sciences were invited to a longitudinal health survey. Survey topics spanned socio-demographic data, students’ health status and behavior, COVID-19 specific impact, concerns, information sources, adherence to containment measures, and trust in government bodies. Group comparisons by gender and multivariate ordinal regression models assessing adherence to restrictions of mobility and social contacts were conducted (n = 2373).

Results
Mean age was 26.4 (SD = 5.6), 70% were female. 43.5% reported some concern about their own health, 2.7% stated major worries. Women experienced more conflicts (p &lt; 0.000) and, enjoyed time with the family more (p &lt; 0.000). Men felt less locked up (p = 0.001). The most frequented COVID-19 information source was public media (48%) and confidence in government bodies was high (82%) for both genders. Men yielded lower adjusted odds (OR; 95%-CI) of adherence regarding the following measures: social distancing (0.68; 0.53–0.87), non-utilization of public transport (0.74; 0.56–0.97), 5-person limit for social gatherings (0.47; 0.35–0.64) and the stay at home rule (0.64; 0.51–0.82).

Conclusion
Early in the pandemic a high degree of adherence was observed in this young academic population. Containment measures restricting movement and social contact yielded considerable differences by gender, information source and perceived susceptibility to the virus. More targeted communication may increase adherence regarding mobility restrictions.
Validation of the Personal Social Capital Scale-16 in Portugal: preliminary data on Portuguese and immigrants
Authors: Nascimento Paulo , Roberto Magda Sofia , Santos Ana Sofia 
Published: February 2021

Summary

Objectives
The Personal Social Capital Scale 16 (PSCS-16) is a self-report measure used to assess social capital, as a social determinant of health. To guarantee validated measures of this construct, the psychometric properties of the Portuguese version of the PSCS-16 were studied.

Methods
The PSCS-16 comprises 16 items, organized in two scales: bonding and bridging social capital. A convenience sample of 280 participants was collected through an online survey. For construct validity, we used confirmatory factor analysis, and convergent and discriminant validity through the average variance extracted (AVE) and correlations. For reliability, we used: The Spearman–Brown split-half and the omega hierarchical coefficient. Correlations were made between the PSCS-16 and socio-demographic variables.

Results
A first-order model depicting two oblique factors was supported, suggesting the use of the two scales. Evidence of convergent validity was achieved: acceptable AVE and associations between social capital and emotional self-disclosure. For discriminant validity, the AVE values surpassed the squared correlation between bonding and bridging, and associations with sexual health were found to be absent. Reliability was good. Additional correlations: A positive association between the education level and bridging social capital and participants with an immigrant status having more bridging social capital.

Conclusions
Preliminary findings support the Portuguese version of PSCS-16 as suitable to evaluate social capital. Contributions are highlighted: the need to study correlates of social capital, particularly crossing migrations, social capital and mental health; and confirming the structure found by measuring its invariance.
‘Alcohol causes cancer’: a difficult message for Australians to swallow
Authors: May Natalie Jane , Eliott Jaklin , Crabb Shona 
Published: February 2021

Summary
Alcohol is a modifiable risk factor for cancer. Public awareness of the link between alcohol and cancer risk is poor; thus, alcohol consumers may be unknowingly putting themselves at increased risk of cancer. One way to raise awareness of alcohol-related cancer is through placing labels warning of cancer risk on alcoholic beverage containers; however, little is known about the impact of such labels. We conducted seven focus groups, comprising participants who self-identified as low-to-moderate alcohol consumers, to gauge public attitudes towards the labels and messages relating to alcohol-related cancer risk. Transcripts of discussions were coded to identify emergent themes. Participants expressed a negative response to the alcohol warning labels, and their talk worked to challenge the legitimacy of alcohol-related cancer messages, and the entities responsible for disseminating the information. These responses functioned to counter any implied recommendation for reduction in speakers’ alcohol consumption. These findings illustrate how the general population make sense of information about health risks, using this knowledge to make decisions about personal behaviour. In combination with other public health initiatives, alcohol-warning labels have the potential to increase awareness of cancer risk and help in the fight against cancer, but any messaging will need to account for probable consumer resistance.
Double Hurwitz Numbers and Multisingularity Loci in Genus 0
Authors: Kazarian Maxim , Lando Sergey , Zvonkine Dimitri 
Published: February 2021

AbstractIn the Hurwitz space of rational functions on ${{\mathbb{C}}}\textrm{P}^1$ with poles of given orders, we study the loci of multisingularities, that is, the loci of functions with a given ramification profile over 0. We prove a recursion relation on the Poincaré dual cohomology classes of these loci and deduce a differential equation on Hurwitz numbers.
Potential applications of halophilic microorganisms for biological treatment of industrial process brines contaminated with aromatics
Authors: Mainka Thomas , Weirathmüller David , Herwig Christoph , Pflügl Stefan 
Published: February 2021

AbstractSaline wastewater contaminated with aromatic compounds can be frequently found in various industrial sectors. Those compounds need to be degraded before reuse of wastewater in other process steps or release to the environment. Halophiles have been reported to efficiently degrade aromatics, but their application to treat industrial wastewater is rare. Halophilic processes for industrial wastewater treatment need to satisfy certain requirements: a continuous process mode, low operational expenditures, suitable reactor systems and a monitoring and control strategy. The aim of this review is to provide an overview of halophilic microorganisms, principles of aromatic biodegradation, and sources of saline wastewater containing aromatics and other contaminants. Finally, process examples for halophilic wastewater treatment and potential process monitoring strategies are discussed. To further illustrate the significant potential of halophiles for saline wastewater treatment and to facilitate development of ready-to-implement processes, future research should focus on scale-up and innovative process monitoring and control strategies.
Effect of Terbufos (Organophosphate) on the Cadaveric Colonization Process: Implications for Postmortem Interval Calculation
Authors: Jales J T , Barbosa T M , Soares V P , Gama R A 
Published: February 2021

Abstract
The determination of necrophagous fly specie’s development time is considered an accurate method for estimating postmortem interval (PMI). However, pesticides and other chemicals can alter the flies’ life cycle, inducing errors in PMI estimation. Thus, this work aimed to evaluate the effect of different doses of Terbufos (Organophosphates) on the temporal dispersion pattern and development of immature dipterans associated with decaying rat carcasses. For this, 150 g female Wistar rats received, via gavage, 200 µl of Terbufos (5 or 10 mg/kg) or distilled water (control) and, after 30 min of administration, the animals were euthanized and distributed in suspended traps to decompose under environmental conditions. The dispersing immatures were collected daily, and their development time was monitored until the emergence of adult flies. After data analysis, it was observed that Terbufos altered 1) the temporal pattern of larval dispersion; 2) the composition and structure of the colonizing assemblage (emerged adults); 3) species’ development time, accelerating or delaying their cycle, depending on the dose used; and 4) the califorids and sarcophagids emergence rate, increasing the mortality of pupae from intoxicated carcasses. Thus, this work demonstrates experimentally that Terbufos directly influences the development of flies with forensic potential and discusses the implications for PMI estimation, which can assist in future investigative processes with suspected poisoning by this organophosphate.
Making Masters Moral: Household Subordinates and Upward Social Discipline in Late Medieval Basel
Authors: Grace Philip 
Published: February 2021

Abstract
This article examines how late medieval non-nuclear household relationships shaped the pursuit of honorable social status. It examines in detail several witness depositions from the Basel municipal court (Schultheissengericht) in the late fifteenth and early sixteenth century. Historians have long noted the concern of householders to regulate the morality of their servants and apprentices. However, the study will demonstrate that subordinates could and did defend their own household honor by policing the morality of their masters and other household members, both through verbal shaming and through the distinctive strategic option of refusing to work. The importance of subordinates as arbiters of honorable status was recognized and exploited by individual masters, guilds, and courts; in the region around Basel, it could even be formalized in the written genre of a Schelmbrief (shame-document). Social discipline was not simply a question of householder-masters controlling their subordinates, but also the reverse.
Golgi-localized membrane protein AtTMN1/EMP12 functions in the deposition of rhamnogalacturonan II and I for cell growth in Arabidopsis
Authors: Hiroguchi Akihiko , Sakamoto Shingo , Mitsuda Nobutaka , Miwa Kyoko 
Published: February 2021

Abstract
Appropriate pectin deposition in cell walls is important for cell growth in plants. Rhamnogalacturonan II (RG-II) is a portion of pectic polysaccharides; its borate crosslinking is essential for maintenance of pectic networks. However, the overall process of RG-II synthesis is not fully understood. To identify a novel factor for RG-II deposition or dimerization in cell walls, we screened Arabidopsis mutants with altered boron (B)-dependent growth. The mutants exhibited alleviated disorders of primary root and stem elongation, and fertility under low B, but reduced primary root lengths under sufficient B conditions. Altered primary root elongation was associated with cell elongation changes caused by loss of function in AtTMN1 (Transmembrane Nine 1)/EMP12, which encodes a Golgi-localized membrane protein of unknown function that is conserved among eukaryotes. Mutant leaf and root dry weights were lower than those of wild-type plants, regardless of B conditions. In cell walls, AtTMN1 mutations reduced concentrations of B, RG-II specific 2-keto-3-deoxy monosaccharides, and rhamnose largely derived from rhamnogalacturonan I (RG-I), suggesting reduced RG-II and RG-I. Together, our findings demonstrate that AtTMN1 is required for the deposition of RG-II and RG-I for cell growth and suggest that pectin modulates plant growth under low B conditions.
Pneumocystis jirovecii among patients with cystic fibrosis and their household members
Authors: Morilla Ruben , Medrano Francisco J , Calzada Ana , Quintana Esther , Campano Elena , Friaza Vicente , Calderón Enrique J , de la Horra Carmen 
Published: February 2021

Abstract

We conducted a pilot study of patients with cystic fibrosis (CF) to assess intra-family transmission of P. jirovecii and compare it with data on other prevalent pathogens such as P. aeruginosa and S. pneumoniae, in which respiratory transmission has already been documented. Oral swab samples from 10 patients with CF and 15 household members were collected at baseline and 2 weeks later. P. aeruginosa and S. pneumoniae were assessed using standardized culture methods and PCR, and P. jirovecii was assessed using real and nested PCR, genotyping the positive samples by direct sequencing. P. aeruginosa cultures were positive for 7/10 (70%) of patients with CF at baseline and was identified by PCR in 8/10 (80%) of cases at baseline and 2 weeks later. S. pneumoniae cultures were negative for all patients, but the microorganism was identified by PCR in two cases. P. jirovecii was detected by real time and nested PCR in 5/10 (50%) of the patients at the two time points. In the household members, P. aeruginosa and P. jirovecii were identified in 7/15 (46.7%), and S. pneumoniae was identified in 8/15 (53,3%). The concordance of positive or negative pairs of patients with CF and their household members was 33.3% (5/15) for P. aeruginosa, 46.7% (7/15) for S. pneumonia and 93.3% (14/15) for P. jirovecii. The concordance for P. jirovecii genotypes among five pairs with available genotype was 100%. This study suggests for the first time the possible transmission of Pneumocystis in the home of patients with CF, indicating that patients and their household members are reservoirs and possible sources of infection.

Lay Summary
This study suggests for the first time the possible transmission of Pneumocystis in the family environment of patients with cystic fibrosis, indicating that patients and their household members are reservoirs and possible sources of this infection.
Evidence of chromospheric molecular hydrogen emission in a solar flare observed by the IRIS satellite
Authors: Mulay Sargam M , Fletcher Lyndsay 
Published: February 2021

ABSTRACT
We have carried out the first comprehensive investigation of enhanced line emission from molecular hydrogen, H2 at 1333.79 Å, observed at flare ribbons in SOL2014-04-18T13:03. The cool H2 emission is known to be fluorescently excited by Si iv 1402.77 Å UV radiation and provides a unique view of the temperature minimum region (TMR). Strong H2 emission was observed when the Si iv 1402.77 Å emission was bright during the flare impulsive phase and gradual decay phase, but it dimmed during the GOES peak. H2 line broadening showed non-thermal speeds in the range 7–18 $\rm {km\,s}^{-1}$, possibly corresponding to turbulent plasma flows. Small red (blue) shifts, up to 1.8 (4.9) $\rm {km\,s}^{-1}$ were measured. The intensity ratio of Si iv 1393.76 Å and Si iv 1402.77 Å confirmed that plasma was optically thin to Si iv (where the ratio = 2) during the impulsive phase of the flare in locations where strong H2 emission was observed. In contrast, the ratio differs from optically thin value of 2 in parts of ribbons, indicating a role for opacity effects. A strong spatial and temporal correlation between H2 and Si iv emission was evident supporting the notion that fluorescent excitation is responsible.