bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comprehensive Analysis of Genome-instability Related lncRNAs and Tumor-immune Microenvironment to Discriminate the Prognosis, Immunotherapy and PARP Inhibitor Responses in Patients with Low-grade Glioma
Authors: Zhou Zijian , Lu Bin , Wei JinHong , Guo Lei , He ZhongMing , Liu Yini , Wang Weimin , Li Luo 
Published: July 2021

Abstract
Background: Previous study revealed that Genome-instability was correlated with tumor-immune microenvironment in cancer. We try to discriminate the prognosis, immunotherapy and poly (ADP)-ribose polymerase (PARP) inhibitor responses through comprehensive analysis of genome-instability related lncRNAs and the tumor-immune microenvironment in patients with low-grade glioma (LGG). Methods and Results: RNAseq data, genome variation profiling data and copy number variation (CNV) data were used to evaluate the genomic instability of LGG patients. Genomic unstable-like (GU-like) and genomic stable-like (GS-like) clusters were identified by hierarchical clustering analysis of 102 genome-instability related lncRNAs (GILncRNAs). GS-like cluster had a tendency to receive better clinical outcome. Patients in GU-like cluster were more likely to respond to immunotherapy, especially anti-PD-1/PD-L1 treatment. PARP inhibitors including Rucaparib and Olaparib will get better therapeutic effects for patients in GU-like cluster. Lasso and Cox regression analysis were utilized to construct the risk model based on GILncRNAs. As for the risk model constructed by 9 GILncRNAs, the overall survival, clinical outcome, immunotherapeutic response, and PARP inhibitor sensitivity were significantly different between patients of high and low-risk groups. Conclusions: The genome-instability related lncRNAs signature involved in our risk model had great advantages in predicting prognosis, immunotherapy and PARP inhibitor response.
Creatinine Reduction Ratio is a Prognostic Factor for Acute Kidney Injury Following Cardiac Surgery with Cardio Pulmonary Bypass: A Single Center Retrospective Cohort Study.
Authors: anzai akiko , takaki shunsuke , Yokoyama Nobuyuki , Kashiwagi Shizuka , Yokose Masashi , Goto Takahisa 
Published: July 2021

Abstract
Background: Acute kidney injury (AKI) after cardiac surgery is common complication, and it is known as risk of death. In the previous study, we reported creatinine reduction ratio (CRR) can be useful as a prognostic factor of AKI. Primary outcome of this study was determination of predictor of AKI after surgery with using perioperative information. Secondary outcome was the reliability of CRR for short and long term outcome among the different nation.Methods: We retrospectively collected cardiac surgical patients with cardiopulmonary bypass from electrical health record. Patients were excluded with insufficient data. AKI was defined by the AKIN and RIFLE criteria, (1) increment of creatinine≧ 0.3mg/dl (2); increment of creatinine≧ 150%. Patients were divided by AKI and non-AKI group. We analyzed two group about the preoperative patients’ data and operative information. CRR was calculated as follows: (preoperative creatinine-postoperative creatinine)/preoperative creatinine. Results: 225 patients data were collected from electrical health record, and analysed. The prognostic factor of AKI-CS was surgery time, CPB time, aorta clamp time, platelet transfusion, and CRR < 20%. In the multivariable logistic analysis, CRR was an independent predictor of AKI. (adjusted odds ratio 0.90[0.87-0.93] p<0.001) However, there were not significant difference in CRR about the rate of new onset of chronic kidney disease (CKD). Conclusions: After cardiac surgery with cardiopulmonary bypass, the CRR has a good diagnostic power for predicting perioperative AKI. However, we cannot use it as a prognostic factor for long term period.
Epidemiology and Phenotypic Characteristics of Dual Molecular Diagnosis Cases in Skeletal Abnormality
Authors: Liu Lian , Sun Liying , Chen Yujun , Yu Chenxi , Huang Yingzhao , Zhao Sen , Du Huakang , Chen Shaoke , Fan Xin , Tian Wen , Wu Zhihong , Qiu Guixing , Zhang Terry Jianguo , Wu Nan 
Published: July 2021

Abstract
Background Skeletal abnormality is a heterogeneous group of disorders that affects the composition and structure of bone and cartilage. In our previous studies, we have revealed that a substantial proportion of cases with early-onset scoliosis could be explained by monogenic disorders such as Marfan syndrome and Ehlers-Danlos syndrome. More recently complex phenotypes caused by more than one genetic defect (i.e., dual molecular diagnosis) have also been reported in skeletal abnormalities. To explore the molecular epidemiology and phenotypic characteristics of dual diagnosis in skeletal abnormalities, we described cases with dual molecular diagnosis from the Deciphering disorders Involving Scoliosis and COmorbidities (DISCO) study.Results In total, 1108 patients with skeletal abnormality from the DISCO study underwent Exome Sequencing. We identified eight probands with dual molecular diagnosis, including three (0.7%) from individuals with early-onset scoliosis (EOS), three (0.5%) from individuals with short stature, and two (2%) from individuals with congenital hand/foot deformity (CHFA). Other skeletal abnormalities observed in these individuals included bone fracture and interphalangeal joint contracture. All the eight probands have dual diagnosis of two autosomal dominant (AD) diseases. A total of 16 variants in 12 genes were identified. A substantial rate (5 of 10) of the identified causal variants were of de novo origin. The frequently observed molecular diagnoses (observed in more than one patient) include Osteogenesis Imperfecta Type I (COL1A1, MIM:166200), Neurofibromatosis, Type I (NF1, MIM:162200) and Marfan Syndrome (FBN1, MIM:154700). These patients with dual molecular diagnosis often present blended phenotypes of two diseases, which significantly complicate their diagnostic process. Conclusions This study revealed the molecular epidemiology and complex diagnostic odyssey of dual molecular diagnosis through analyzing the clinical traits of skeletal abnormalities in eight patients.
Transcriptomics Analysis of Daheng Broilers Reveals that PLIN2 Regulates Chicken Preadipocyte Proliferation, Differentiation and Apoptosis
Authors: Li Jingjing , Yang Chaowu , Ren Peng , Lin Zhongzhen , Zhang Donghao , Jiang Xiaosong , Wang Li , Liu Yiping 
Published: July 2021

Abstract
Intramuscular fat content, an important meat quality trait, strongly affects flavor, juiciness, and tenderness. Sex hormones regulate lipid metabolism, and female hormones stimulate fat deposition, thereby making the female chickens always fatter than males. In this study, the effect of sex on IMF deposition was screened following transcriptomics in chickens. Results confirmed significantly higher IMF content of 150-day female chickens as compared to the male chickens. The female chickens manifested higher serum TG, LDL-C, and VLDL, and significantly lower HDL-C contents than male chickens. Moreover, differential expression of genes involved in lipid metabolism were obtained in the muscle and liver between female and male chicken, which could partly interpret the possible reasons for the sex-mediated differences of IMF content. Cellular results revealed that inhibition of PLIN2 significantly inhibited chicken preadipocyte proliferation and induces apoptosis of preadipocytes, as well as promoted adipocyte differentiation.
Polycystic Ovary Syndrome Phenotype Does Not Have Impact on Oocyte Morphology
Authors: Uk Audrey , Decanter Christine , Grysole Camille , Keller Laura , Béhal Hélène , Silva Mauro , Dewailly Didier , Robin Geoffroy , barbotin anne-laure 
Published: July 2021

Abstract
Purpose The primary objective of the present study of women participating in an ICSI program was to determine whether the morphologic quality of oocytes was related to the polycystic ovary syndrome (PCOS) phenotype.Methods We performed a retrospective cohort study in the IVF unit at the Lille University Medical Center (Lille, France) between 2006 and 2015. Oocyte morphology (fragmented first polar body, abnormal zona pellucida, large perivitelline space, material in perivitelline space, abnormal shape of oocyte, granular cytoplasm and intracytoplasmic vacuoles) was evaluated in PCOS women and according to different subgroup (depending on the presence or absence of the cardinal features polycystic ovarian morphology (PCOM), hyperandrogenism (HA), and oligo-anovulation (OA)).Results A total of 1496 metaphase II oocytes (n=602 for phenotype A combining PCOM + HA + OA, n=462 oocytes for phenotype C: PCOM + HA, and n=432 for phenotype D: PCOM + OA) were assessed. The phenotypes A, C and D did not differ significantly with regard to the proportion of normal oocytes (adjusted percentages (95%CI): 35.2% (31.5% to 39.1%), 25.8% (21.9% to 29.9%) and 34.0% (29.7% to 38.6%), respectively: adjusted p=0.13). Likewise, there were no significant intergroup differences in oocyte morphology. The ICSI outcome was not significantly associated with the PCOS phenotype.Conclusion The present study is the first to show that the PCOS phenotype (notably the presence vs. absence of OA and/or HA) is not significantly associated with the morphological quality of oocytes.
Molecular Genotypic Diversity of Populations of Brinjal Shoot and Fruit Borer, Leucinodes Orbonalis and Development of SCAR Marker for Pesticide Resistance
Authors: Murali Palraju , Hilda Karuppiah , Ramakrishnan Muthusamy , Ganesh Arumugam , Bhuvaragavan Sreeramulu , Janarthanan Sundaram 
Published: July 2021

Abstract
The brinjal shoot and fruit borer, Leucinodes orbonalis is a destructive pest of Solanum melongena. The control of L. orbonalis with extensive application of synthetic chemical insecticides resulted in the development of resistance with known genetic heterogeneity among populations. Understanding the genetic diversity of their populations is important in developing strategies for their management. The present investigation was performed to characterize populations of L. orbonalis for their genetic diversity in the entire region of Tamil Nadu, South India using random amplified polymorphic DNA (RAPD) primers as a tool of the molecular marker. Among sixty random 10-mer primers, only ten primers generated reproducible and scorable banding profile. Among the ten different random primers, the primers namely OPG 7, OPG 8, OPS 2 and OPS 7 generated the highest genetic variation with over 80% genetic polymorphism. Phylogram analysis produced 18 clusters with 8 major and 10 minor clusters. Cluster analysis, statistical fitness, population structure and analysis of molecular variance confirmed the significant genetic variation among different populations. A trait specific marker obtained through RAPD was cloned, sequenced and used to develop a stable diagnostic SCAR marker for DNA fingerprinting to distinguish the populations. Amplification of this locus in the samples of 20 different populations indicated recognition of the trait for pesticide resistance in 12 populations. The results suggest that the biochemical nature of host plant varieties of this insect pest and variation in the application of different insecticides are essential contributing factors for the genotypic variations observed among populations of L. orbonalis.
A New Method for Measuring Stress Inside Movable Element in Contact
Authors: Yu Gonggui , He Jun , Hoang Van Cuong , Huang Ping 
Published: July 2021

Abstract
The subsurface stress plays an important role in the damage of the movable contact element, but most subsurface stresses are obtained with numerical calculations according to the contact mechanics. In the present paper, a new method to measure the subsurface stress of the movable element is proposed with using photoelastic technology. Although the technology has been widely used in measuring the stress of the static elements, it is seldom used in the moving body because the observed point is moving. After the experimental tester is introduced in detail, the principles of the photoelastic technology are presented. The tester is designed to be able to working in the three conditions, the static, the rolling and the sliding in the line or surface contacts. The experimental results, that is the interference fringes, of the three states are then presented in the different loads and the rotational speeds. Because the fringe figures indicate the maximum shear stress distribution in the body of the moving element, we can find what the real stress distribution in the rolling or sliding element is alike.
Prognostic and Therapeutic Potential of The OIP5 Network in Papillary Renal Cell Carcinoma
Authors: Chow Mathilda , Gu Yan , He Lizhi , Lin Xiaozeng , Dong Ying , Mei Wenjuan , Kapoor Anil , Tang Damu 
Published: July 2021

Abstract

Background: Papillary renal cell carcinoma (pRCC) is an aggressive but minor type of RCC compared to the main RCC type, clear cell RCC. The current understanding and management of pRCC remain poor. OIP5 possesses oncogenic functions; its contributions to pRCC remain unknown.Methods: OIP5 expression in pRCC at both the protein and mRNA levels was determined using tissue microarray and TCGA dataset. OIP5 was ectopically expressed in metastatic ACHN pRCC cells; xenografts were performed with gene expression profiled by RNA-seq. Differentially expressed genes (DEGs) were analyzed for prognostic potential and impact on pRCC. The effect of PLK1, an OIP5-related DEG, on pRCC tumor growth in vivo was examined.Results: OIP5 expression is upregulated in pRCC. The upregulation associates with pRCC adverse features (T1P<T2P<CIMP, Stage1+2<Stage 3<Stage 4, and N0<N1) and effectively stratifies the fatality risk. OIP5 promotes ACHN pRCC cell proliferation and xenograft formation. RNA-seq reveals network alterations related to immune regulation, metabolism, and hypoxia in ACHN OIP5 tumors compared to empty vector tumors. A set of DEGs was derived from ACHN OIP5 xenografts and primary pRCCs (n=282) contingent to OIP5 upregulation; both DEG sets share 66 overlap genes. Overlap66 effectively predicts overall survival (p<2e-16) and relapse (p<2e-16) possibilities. The prediction is associated with a good out-of-sample performance, supporting its clinical applications. High-risk tumors stratified by Overlap66 risk score possess an immune suppressive environment, evident by elevations in Treg cells and PD1 expression in CD8 T cells. Upregulation of PLK1 occurs in both xenografts and primary pRCC tumors with OIP5 elevations. PLK1 displays a synthetic lethality relationship with OIP5; PLK1 inhibitor BI2356 causes G2/M arrest in ACHN OIP5 cells in vitro and significantly inhibits the growth of xenografts formed by ACHN OIP5 cells in vivo. Conclusions: Our research reveals that OIP5 and its network possess robust prognostic and therapeutic potentials; the prognostic value of Overlap66 and the therapeutic potential of PLK1 inhibitors may pave the way for developing personalized medicine for pRCC management.
Title: Efficacy of Psychosocial Group Treatment for Post-traumatic Stress Disorder among Genocide Survivors in Rwanda, 25 Years After 1994 Genocide Against Tutsi.
Authors: SIMBI Clarisse Marie Claudine , Zhang Yuhong , Wang Zhizhong 
Published: July 2021

Abstract
Background: Prior studies indicated that post-traumatic stress disorder is becoming a global health concern even though still poorly known and treated. In the aftermath of 1994 Genocide against Tutsi, studies found high rates of depressive and anxious symptoms along with PTSD among genocide survivors. Due to the highest cruelty in which the Genocide was committed, genocide survivors still need high special humanitarian services, of those including specialized health care services. The aim of this study was to assess the efficacy of psychosocial group therapies created by AVEGA Agahozo in reducing PTSD symptoms among Genocide survivors in Rwanda, 25 years after 1994 Genocide against Tutsi.Methods: We conducted a comparative cross-sectional study design with a sample of 98 genocide survivors who received group therapy by AVEGA Agahozo. We used a multi-stage random sampling method to select participants and 7 trained psychologists interviewed genocide survivors about their PTSD status before and after treatment using Diagnostic and Statistical Manual of Mental Disorders, 5th Edition. The analysis was performed using SPSS version 17.1.Results: The results showed that women were 97.96% and men presented 2.04% of all participants because AVEGA Agahozo mainly focuses on helping women survivors who lost their husbands in Genocide and previous findings also concluded that women are very prone to suffer from PTSD than men. Paired t-test results showed significant differences between symptoms, before and after treatment (P<0.001 in all pairs). Cohen's d results also showed high effect sizes (d>0.5), only in pair 8 where the difference appears to be less significant (d=0.28). The descriptive statistics showed that the severity of PTSD symptoms dramatically reduced after treatment. But this difference of severity is only statistically significant among five (5) PTSD symptoms.: (Marked physiological reactivity after exposure to trauma-related stimuli [P=0.045, x2=38.111]; inability to recall key features of the traumatic event [P<001, x2=56.309]; persistent negative trauma-related emotions [P=0.013, x2=43.184]; self-destructive or reckless behavior [P=0.041, x2=38.535]; hypervigilance [P=0.020, x2=41.596]. Conclusion: Psychosocial group therapies created by AVEGA Agahozo effectively alleviate post-traumatic stress disorder symptoms and severity among genocide survivors.
Team-Based Learning in a Physiology Course To Prepare for The International Inter-Medical School Physiology Written Quiz in Kunming Medical University
Authors: Bian Hui , Bian Yan 
Published: July 2021

Abstract
Background

International Inter-medical School Physiology Quiz (IMSPQ) is a global event that purportedly engages students in team work, critical thinking and active learning of physiology. Team-based learning (TBL) is an interactive analytic teaching approach and has been used extensively in medical education. However, there is little evidence of its use in preparing for IMSPQ. The purpose of the present study was to investigate whether a TBL approach is effective to prepare for the written section of IMSPQ.
Methods

Five students from Grade 2014 majoring in Clinical Medicine, were selected, based on academic performance in physiology and English, to be taught in a TBL physiology course to prepare for the written section of 14th IMSPQ.
Results

In 2016, this TBL teaching method worked well and for the first time the team from Kunming Medical University entered the top forty teams at the written competition of 14th IMSPQ in Yogyakarta.
Conclusion

TBL method was effective to prepare for the IMSPQ in Kunming Medical University and provided an improved team performance in the written section of 14th IMSPQ.
Contribution In Determining The Fatigue Endurance of Slide Diamond Burnishing AISI52100 Steel Components
Authors: Taamallah Ouahiba , Hamadache Hamid , Amirat Abdelaziz , Boussaid Ouzine 
Published: July 2021

Abstract
The present paper is an investigation on the effect of slide diamond burnishing on the fatigue endurance of a component made of AISI52100 steel. Burnishing operation has been performed on cylindrical specimens using optimal parameters statistically selected such as burnishing force, burnishing feed and number of tool passes. Bending fatigue tests in air at R= -1 and S-N curves have been plotted by incrementing the applied stress from a maximum stress level of about 66% of the ultimate tensile stress to a stress value below which fatigue does not occur. Results show that slide diamond burnishing has increased the fatigue resistance comparing to unburnished specimens. The fatigue endurance is respectively 222 MPa and 190 MPa. At high stress levels, the fatigue resistance improvement is clearly observed and the fatigue trends are in good agreement with those reported in literature. However, the present results are slightly lower and that is attributed to the shoulder fillet value of the specimen. For small fillet radius, fatigue resistance is lowered.
Exosomal Long Noncoding RNA HOXD-AS1 Promotes Prostate Cancer Metastasis via miR-361-5p/FOXM1 Axis
Authors: Jiang Yongming , Zhao Hui , Chen Yuxiao , Li Kangjian , Chen Jianheng , Li Tianjie , Zhang Baiyu , Guo Caifen , Qing Liangliang , Shen Jihong , Liu Xiaodong , Gu Peng 
Published: July 2021

Abstract
Background: Development of distant metastasis is the main cause of deaths in prostate cancer (PCa) patients. Understanding the mechanism of PCa metastasis is of utmost importance to improve its prognosis. The role of exosomal long noncoding RNA (lncRNA) has been reported yet fully understood in the metastasis of PCa. Methods: Co-culture assay, in-situ hybridization and quantitative PCR (qPCR) were applied to identify the presence of HOXD-AS1 overexpressing exosomes secreted by of castration resistant prostate cancer (CRPC) cells. Migration and wound healing assays were carried out to evaluate the biological function of exosomal HOXD-AS1. Then a mouse model was used to elucidate the effect of exosomal HOXD-AS1 on distant metastasis of PCa in vivo. Further RNA immunoprecipitation and luciferase assay were applied to identify the interaction between HOXD-AS1 and miR-361-5p. Moreover, rescue experiment was conducted to prove the binding of exosomal HOXD-AS1 and miR-361-5p. Last but not least, the application of serum exosomal HOXD-AS1 as a diagnostic and prognostic biomarker for PCa was evaluated. Results: We discovered the exosomal lncRNA HOXD-AS1 is upregulated in CRPC cell line derived exosomes and serum exosomes from metastatic PCa patients, which correlated with its tissue expression. Further investigation confirmed exosomal HOXD-AS1 promotes prostate cancer cell metastasis in vitro and in vivo by inducing metastasis associated phenotype. Mechanistically exosomal HOXD-AS1 was internalized directly by PCa cells, acting as competing endogenous RNA (ceRNA) to modulate the miR-361-5p/FOXM1 axis, therefore promoting PCa metastasis. In addition, we found that serum exosomal HOXD-AS1 was upregulated in metastatic PCa patients, especially those with high volume disease. And it is correlated closely with Gleason Score, distant and nodal metastasis, Prostatic specific antigen (PSA) recurrence free survival and progression free survival (PFS). Conclusions: Our study sheds a new insight into the regulation of PCa distant metastasis by exosomal HOXD-AS1 mediated miR-361-5p/FOXM1 axis, and provided a promising liquid biopsy biomarker as well as therapeutic target to the detection and treatment of metastatic PCa.