bluuebunny/crossref_metadata_2025_split huggingface dataset:
Markets, infrastructures and infrastructuring markets
Authors: Araujo Luis , Mason Katy 
Published: November 2021

AbstractDespite a growing understanding of market infrastructures—the rules and socio-material arrangements that enable agreements on the properties of goods, and the calculation of value, equivalence and exchange—we know little of what lies beneath the arrangements that underpin and are implicated in exchange. The socio-material lens has done much to explain how specific assemblages circulate information and goods, but has done little to explain how different infrastructures configure relations between dispersed market practices. Using the history of the development of the market for market research we show how knowledge-based infrastructures constitute markets as knowledge objects: new expertise emerged through alliances between academia, government, and private actors form a new occupation embodied in specialist agencies that set themselves up in an infrastructural relation to marketing practices. Our conceptualization of markets as knowledge objects extends extant understandings of markets by showing how: (1) extant knowledge-based infrastructures are drawn on to construct new markets; (2) infrastructural relations emerge between different markets to constitute multiple systems of provision and demand, leading to an increasingly valuable knowledge infrastructure; and (3) organized practices in one market are often heavily reliant on connections to other markets, including knowledge-based infrastructures such as market research services.
A review on low salinity waterflooding in carbonates: challenges and future perspective
Authors: Nande Soumitra B. , Patwardhan Samarth D. 
Published: November 2021

AbstractLow salinity waterflooding has gained significant attention and importance in the last decade, as it is seen as an impactful method for recovery of additional oil from carbonate reservoirs. Existing literature does not do justice to the underlying mechanisms that aid in the recovery of additional oil from such rock types. In this paper, we present a comprehensive review of the research conducted on low salinity waterflooding in carbonates and further provide a detailed and critical analysis on the same. The intention of this paper is also to present a condensed research summary on the said topic, and to chart out a detailed roadmap for future work, thereby opening the possibilities of new avenues of research in the field.
Current practices and evaluation of barriers and facilitators to surgical site infection prevention measures in Jimma, Ethiopia
Authors: Berman Leigh R. , Lang Andrew , Gelana Beshea , Starke Samuel , Siraj Dawd , Yilma Daniel , Shirley Daniel 
Published: November 2021

Abstract

Objective:
Surgical site infections (SSIs) greatly burden healthcare systems around the world, particularly in low- and middle-income countries. We sought to employ the Systems Engineering Initiative for Patient Safety (SEIPS) model to better characterize SSI prevention practices and factors affecting adherence to prevention guidelines at Jimma University Medical Center (JUMC).

Design:
Our cross-sectional study consisted of semistructured interviews designed to elicit perceptions of and barriers and facilitators to SSI prevention among surgical staff and observations of current preoperative, perioperative, and postoperative SSI prevention practices in surgical cases. Interviews were recorded, manually transcribed, and thematically coded within the SEIPS framework. Trained observers recorded compliance with the World Health Organization’s SSI prevention recommendations.

Setting:
A tertiary-care hospital in Jimma, Ethiopia.

Participants:
Surgical nurses, surgeons, and anesthetists at JUMC.

Results:
Within 16 individual and group interviews, participants cited multiple barriers to SSI prevention including shortages of water and antiseptic materials, lack of clear SSI guidelines and training, minimal Infection Prevention Control (IPC) interaction with surgical staff, and poor SSI tracking. Observations from nineteen surgical cases revealed high compliance with antibiotic prophylaxis (94.7%), hand scrubbing (100%), sterile gloves and instrument use (100%), incision site sterilization (100%), and use of surgical safety checklist (94.7%) but lower compliance with preoperative bathing (26.3%), MRSA screening (0%), and pre- and postoperative glucose (0%, 10.5%) and temperature (57.9%, 47.3%) monitoring.

Conclusions:
Utilizing the SEIPS model helped identify institution-specific barriers and facilitators that can inform targeted interventions to increase compliance with currently underperformed SSI prevention practices at JUMC.
Engaging armed groups at the International Committee of the Red Cross: Challenges, opportunities and COVID-19
Authors: Herbet Irénée , Drevon Jérôme 
Published: November 2021

AbstractThis article examines the presence of 605 armed groups in today's conflict environment by bringing new evidence based on internal research. It looks in particular at the way these non-State entities provide varying degrees of services to the population in the spaces that they control, and how this might impact the way a humanitarian organization like the ICRC engages with them in a dialogue over time. This model of analysis is then used to situate and better explain armed groups’ positions on the COVID crisis.
Light management with quantum nanostructured dots-in-host semiconductors
Authors: Alexandre M. , Águas H. , Fortunato E. , Martins R. , Mendes M. J. 
Published: November 2021

AbstractInsightful knowledge on quantum nanostructured materials is paramount to engineer and exploit their vast gamut of applications. Here, a formalism based on the single-band effective mass equation was developed to determine the light absorption of colloidal quantum dots (CQDs) embedded in a wider bandgap semiconductor host, employing only three parameters (dots/host potential barrier, effective mass, and QD size). It was ascertained how to tune such parameters to design the energy level structure and consequent optical response. Our findings show that the CQD size has the biggest effect on the number and energy of the confined levels, while the potential barrier causes a linear shift of their values. While smaller QDs allow wider energetic separation between levels (as desired for most quantum-based technologies), the larger dots with higher number of levels are those that exhibit the strongest absorption. Nevertheless, it was unprecedently shown that such quantum-enabled absorption coefficients can reach the levels (104–105 cm−1) of bulk semiconductors.
GeSnOI mid-infrared laser technology
Authors: Wang Binbin , Sakat Emilie , Herth Etienne , Gromovyi Maksym , Bjelajac Andjelika , Chaste Julien , Patriarche Gilles , Boucaud Philippe , Boeuf Frédéric , Pauc Nicolas , Calvo Vincent , Chrétien Jérémie , Frauenrath Marvin , Chelnokov Alexei , Reboud Vincent , Hartmann Jean-Michel , El Kurdi Moustafa 
Published: November 2021

AbstractGeSn alloys are promising materials for CMOS-compatible mid-infrared lasers manufacturing. Indeed, Sn alloying and tensile strain can transform them into direct bandgap semiconductors. This growing laser technology however suffers from a number of limitations, such as poor optical confinement, lack of strain, thermal, and defects management, all of which are poorly discussed in the literature. Herein, a specific GeSn-on-insulator (GeSnOI) stack using stressor layers as dielectric optical claddings is demonstrated to be suitable for a monolithically integration of planar Group-IV semiconductor lasers on a versatile photonic platform for the near- and mid-infrared spectral range. Microdisk-shape resonators on mesa structures were fabricated from GeSnOI, after bonding a Ge0.9Sn0.1 alloy layer grown on a Ge strain-relaxed-buffer, itself on a Si(001) substrate. The GeSnOI microdisk mesas exhibited significantly improved optical gain as compared to that of conventional suspended microdisk resonators formed from the as-grown layer. We further show enhanced vertical out-coupling of the disk whispering gallery mode in-plane radiation, with up to 30% vertical out-coupling efficiency. As a result, the GeSnOI approach can be a valuable asset in the development of silicon-based mid-infrared photonics that combine integrated sources in a photonic platform with complex lightwave engineering.
PTEN alleviates maladaptive repair of renal tubular epithelial cells by restoring CHMP2A-mediated phagosome closure
Authors: Wang Huizhen , Wang Yifan , Wang Xin , Huang Huimi , Bao Jingfu , Zhong Wenhui , Li Aiqing 
Published: November 2021

AbstractPhosphatase and Tensin Homolog on chromosome Ten (PTEN) has emerged as a key protein that governs the response to kidney injury. Notably, renal adaptive repair is important for preventing acute kidney injury (AKI) to chronic kidney disease (CKD) transition. To test the role of PTEN in renal repair after acute injury, we constructed a mouse model that overexpresses PTEN in renal proximal tubular cells (RPTC) by crossing PTENfl-stop-fl mice with Ggt1-Cre mice. Mass spectrometry-based proteomics was performed after subjecting these mice to ischemia/reperfusion (I/R). We found that PTEN was downregulated in renal tubular cells in mice and cultured HK-2 cells subjected to renal maladaptive repair induced by I/R. Renal expression of PTEN negatively correlated with NGAL and fibrotic markers. RPTC-specific PTEN overexpression relieved I/R-induced maladaptive repair, as indicated by alleviative tubular cell damage, apoptosis, and subsequent renal fibrosis. Mass spectrometry analysis revealed that differentially expressed proteins in RPTC-specific PTEN overexpression mice subjected to I/R were significantly enriched in phagosome, PI3K/Akt, and HIF-1 signaling pathway and found significant upregulation of CHMP2A, an autophagy-related protein. PTEN deficiency downregulated CHMP2A and inhibited phagosome closure and autolysosome formation, which aggravated cell injury and apoptosis after I/R. PTEN overexpression had the opposite effect. Notably, the beneficial effect of PTEN overexpression on autophagy flux and cell damage was abolished when CHMP2A was silenced. Collectively, our study suggests that PTEN relieved renal maladaptive repair in terms of cell damage, apoptosis, and renal fibrosis by upregulating CHMP2A-mediated phagosome closure, suggesting that PTEN/CHMP2A may serve as a novel therapeutic target for the AKI to CKD transition.
Single cell multi-omic analysis identifies a Tbx1-dependent multilineage primed population in murine cardiopharyngeal mesoderm
Authors: Nomaru Hiroko , Liu Yang , De Bono Christopher , Righelli Dario , Cirino Andrea , Wang Wei , Song Hansoo , Racedo Silvia E. , Dantas Anelisa G. , Zhang Lu , Cai Chen-Leng , Angelini Claudia , Christiaen Lionel , Kelly Robert G. , Baldini Antonio , Zheng Deyou , Morrow Bernice E. 
Published: November 2021

AbstractThe poles of the heart and branchiomeric muscles of the face and neck are formed from the cardiopharyngeal mesoderm within the pharyngeal apparatus. They are disrupted in patients with 22q11.2 deletion syndrome, due to haploinsufficiency of TBX1, encoding a T-box transcription factor. Here, using single cell RNA-sequencing, we now identify a multilineage primed population within the cardiopharyngeal mesoderm, marked by Tbx1, which has bipotent properties to form cardiac and branchiomeric muscle cells. The multilineage primed cells are localized within the nascent mesoderm of the caudal lateral pharyngeal apparatus and provide a continuous source of cardiopharyngeal mesoderm progenitors. Tbx1 regulates the maturation of multilineage primed progenitor cells to cardiopharyngeal mesoderm derivatives while restricting ectopic non-mesodermal gene expression. We further show that TBX1 confers this balance of gene expression by direct and indirect regulation of enriched genes in multilineage primed progenitors and downstream pathways, partly through altering chromatin accessibility, the perturbation of which can lead to congenital defects in individuals with 22q11.2 deletion syndrome.
Measuring the photoelectron emission delay in the molecular frame
Authors: Rist Jonas , Klyssek Kim , Novikovskiy Nikolay M. , Kircher Max , Vela-Pérez Isabel , Trabert Daniel , Grundmann Sven , Tsitsonis Dimitrios , Siebert Juliane , Geyer Angelina , Melzer Niklas , Schwarz Christian , Anders Nils , Kaiser Leon , Fehre Kilian , Hartung Alexander , Eckart Sebastian , Schmidt Lothar Ph. H. , Schöffler Markus S. , Davis Vernon T. , Williams Joshua B. , Trinter Florian , Dörner Reinhard , Demekhin Philipp V. , Jahnke Till 
Published: November 2021

AbstractHow long does it take to emit an electron from an atom? This question has intrigued scientists for decades. As such emission times are in the attosecond regime, the advent of attosecond metrology using ultrashort and intense lasers has re-triggered strong interest on the topic from an experimental standpoint. Here, we present an approach to measure such emission delays, which does not require attosecond light pulses, and works without the presence of superimposed infrared laser fields. We instead extract the emission delay from the interference pattern generated as the emitted photoelectron is diffracted by the parent ion’s potential. Targeting core electrons in CO, we measured a 2d map of photoelectron emission delays in the molecular frame over a wide range of electron energies. The emission times depend drastically on the photoelectrons’ emission directions in the molecular frame and exhibit characteristic changes along the shape resonance of the molecule.
Independent infections of porcine deltacoronavirus among Haitian children
Authors: Lednicky John A. , Tagliamonte Massimiliano S. , White Sarah K. , Elbadry Maha A. , Alam Md. Mahbubul , Stephenson Caroline J. , Bonny Tania S. , Loeb Julia C. , Telisma Taina , Chavannes Sonese , Ostrov David A. , Mavian Carla , Beau De Rochars Valery Madsen , Salemi Marco , Morris J. Glenn 
Published: November 2021

AbstractCoronaviruses have caused three major epidemics since 2003, including the ongoing SARS-CoV-2 pandemic. In each case, the emergence of coronavirus in our species has been associated with zoonotic transmissions from animal reservoirs1,2, underscoring how prone such pathogens are to spill over and adapt to new species. Among the four recognized genera of the family Coronaviridae, human infections reported so far have been limited to alphacoronaviruses and betacoronaviruses3–5. Here we identify porcine deltacoronavirus strains in plasma samples of three Haitian children with acute undifferentiated febrile illness. Genomic and evolutionary analyses reveal that human infections were the result of at least two independent zoonoses of distinct viral lineages that acquired the same mutational signature in the genes encoding Nsp15 and the spike glycoprotein. In particular, structural analysis predicts that one of the changes in the spike S1 subunit, which contains the receptor-binding domain, may affect the flexibility of the protein and its binding to the host cell receptor. Our findings highlight the potential for evolutionary change and adaptation leading to human infections by coronaviruses outside of the previously recognized human-associated coronavirus groups, particularly in settings where there may be close human–animal contact.
SPME-LC/MS-based serum metabolomic phenotyping for distinguishing ovarian cancer histologic subtypes: a pilot study
Authors: Olkowicz Mariola , Rosales-Solano Hernando , Kulasingam Vathany , Pawliszyn Janusz 
Published: November 2021

AbstractEpithelial ovarian cancer (EOC) is the most common cause of death from gynecological cancer. The outcomes of EOC are complicated, as it is often diagnosed late and comprises several heterogenous subtypes. As such, upfront treatment can be highly challenging. Although many significant advances in EOC management have been made over the past several decades, further work must be done to develop early detection tools capable of distinguishing between the various EOC subtypes. In this paper, we present a sophisticated analytical pipeline based on solid-phase microextraction (SPME) and three orthogonal LC/MS acquisition modes that facilitates the comprehensive mapping of a wide range of analytes in serum samples from patients with EOC. PLS-DA multivariate analysis of the metabolomic data was able to provide clear discrimination between all four main EOC subtypes: serous, endometrioid, clear cell, and mucinous carcinomas. The prognostic performance of discriminative metabolites and lipids was confirmed via multivariate receiver operating characteristic (ROC) analysis (AUC value > 88% with 20 features). Further pathway analysis using the top 57 dysregulated metabolic features showed distinct differences in amino acid, lipid, and steroids metabolism among the four EOC subtypes. Thus, metabolomic profiling can serve as a powerful tool for complementing histology in classifying EOC subtypes.
SuperSelective primer pairs for sensitive detection of rare somatic mutations
Authors: Kramer Fred Russell , Vargas Diana Yaneth 
Published: November 2021

AbstractSuperSelective primers, by virtue of their unique design, enable the selective exponential amplification of rare DNA fragments containing somatic mutations in the presence of abundant closely related wild-type DNA fragments. However, when a SuperSelective primer is used in conjunction with a conventional reverse primer, linear amplification of the abundant wild-type fragments occurs, and this may lead to a late arising signal that can be confused with the late arising signal from the rare mutant fragments. We have discovered that the use of a pair of SuperSelective primers, one specific for the target mutation in a plus strand, and the other specific for the same mutation in the complementary minus strand, but both possessing 3′-terminal nucleotides that are complementary to the mutation, significantly suppresses the linear amplification of the related wild-type sequence, and prevents the generation of false mutant sequences due to mis-incorporation by the DNA polymerase. As a consequence, the absence of mutant fragments in a sample does not give rise to a false-positive signal, and the presence of mutant fragments in a sample is clearly distinguishable as a true-positive signal. The use of SuperSelective primer pairs should enhance the sensitivity of multiplex PCR assays that identify and quantitate somatic mutations in liquid biopsies obtained from patients with cancer, thereby enabling the choice of a targeted therapy, the determination of its effectiveness over time, and the substitution of a more appropriate therapy as new mutations arise.
Detection of cancer stem cells by EMT-specific biomarker-based peptide ligands
Authors: Chen Yi-An , Ho Cheau-Ling , Ku Min-Tzu , Hwu Luen , Lu Cheng-Hsiu , Chiu Sain-Jhih , Chang Wen-Yi , Liu Ren-Shyan 
Published: November 2021

AbstractThe occurrence of epithelial-mesenchymal transition (EMT) within tumors, which enables invasion and metastasis, is linked to cancer stem cells (CSCs) with drug and radiation resistance. We used two specific peptides, F7 and SP peptides, to detect EMT derived cells or CSCs. Human tongue squamous carcinoma cell line-SAS transfected with reporter genes was generated and followed by spheroid culture. A small molecule inhibitor-Unc0642 and low-dose ionizing radiation (IR) were used for induction of EMT. Confocal microscopic imaging and fluorescence-activated cell sorting analysis were performed to evaluate the binding ability and specificity of peptides. A SAS xenograft mouse model with EMT induction was established for assessing the binding affinity of peptides. The results showed that F7 and SP peptides not only specifically penetrated into cytoplasm of SAS cells but also bound to EMT derived cells and CSCs with high nucleolin and vimentin expression. In addition, the expression of CSC marker and the binding of peptides were increased in tumors isolated from Unc0642/IR-treated groups. Our study demonstrates the potential of these peptides for detecting EMT derived cells or CSCs and might provide an alternative isolation method for these subpopulations within the tumor in the future.
Principles governing control of aggregation and dispersion of aqueous graphene oxide
Authors: Suter James L. , Coveney Peter V. 
Published: November 2021

AbstractControlling the structure of graphene oxide (GO) phases and their smaller analogues, graphene (oxide) quantum dots (GOQDs), is vitally important for any of their widespread intended applications: highly ordered arrangements of nanoparticles for thin-film or membrane applications of GO, dispersed nanoparticles for composite materials and three-dimensional porous arrangements for hydrogels. In aqueous environments, it is not only the chemical composition of the GO flakes that determines their morphologies; external factors such as pH and the coexisting cations also influence the structures formed. By using accurate models of GO that capture the heterogeneity of surface oxidation and very large-scale coarse-grained molecular dynamics that can simulate the behaviour of GO at realistic sizes of GOQDs, the driving forces that lead to the various morphologies in aqueous solution are resolved. We find the morphologies are determined by a complex interplay between electrostatic, $${\pi }$$
π
–$${\pi }$$
π
and hydrogen bonding interactions. Assembled morphologies can be controlled by changing the degree of oxidation and the pH. In acidic aqueous solution, the GO flakes vary from fully aggregated over graphitic domains to partial aggregation via hydrogen bonding between hydroxylated domains, leading to the formation of planar extended flakes at high oxidation ratios and stacks at low oxidation ratios. At high pH, where the edge carboxylic acid groups are deprotonated, electrostatic repulsion leads to more dispersion, but a variety of aggregation behaviour is surprisingly still observed: over graphitic regions, via hydrogen bonding and “face-edge” interactions. Calcium ions cause additional aggregation, with a greater number of “face-face” and “edge-edge” aggregation mechanisms, leading to irregular aggregated structures. “Face-face” aggregation mechanisms are enhanced by the GO flakes possessing distinct domains of hydroxylated and graphitic regions, with $${\pi }$$
π
–$${\pi }$$
π
and hydrogen bonding interactions prevalent between these regions on aggregated flakes respectively. These findings furnish explanations for the aggregation characteristics of GO and GOQDs, and provide computational methods to design directed synthesis routes for self-assembled and associated applications.
3D hotspot matrix of Au nanoparticles on Au island film with a spacer layer of dithiol molecules for highly sensitive surface-enhanced Raman spectroscopy
Authors: Lee Dong-Jin , Kim Dae Yu 
Published: November 2021

AbstractEngineering of efficient plasmonic hotspots has been receiving great attention to enhance the sensitivity of surface-enhanced Raman scattering (SERS). In the present study, we propose a highly sensitive SERS platform based on Au nanoparticles (AuNPs) on Au island film (AuIF) with a spacer layer of 1,4-benzenedimethanethiol (BDMT). The three-dimensional (3D) hotspot matrix has been rationally designed based on the idea of employing 3D hotspots with a vertical nanogap between AuIF and AuNPs after generating large area two-dimensional hotspots of AuIF. AuNPs@BDMT@AuIF are fabricated by functionalizing BDMT on AuIF and then immobilizing AuNPs. The SERS performance is investigated with Rhodamine 6G as a probe molecule and the determined enhancement factor is 1.3 × 105. The AuNPs@BDMT@AuIF are then employed to detect thiram, which is used as a fungicide, with a detection limit of 13 nM. Our proposed platform thus shows significant potential for use in highly sensitive SERS sensors.