bluuebunny/crossref_metadata_2025_split huggingface dataset:
The impact of housing conditions on porcine mesenchymal stromal/stem cell populations differ between adipose tissue and skeletal muscle
Authors: Quéméner Audrey , Dessauge Frédéric , Perruchot Marie-Hélène , Le Floc’h Nathalie , Louveau Isabelle 
Published: June 2021

ABSTRACTBackgroundIn pigs, the ratio between lean mass and fat mass in the carcass determines production efficiency and is strongly influenced by the number and size of cells in tissues. During growth, the increase in the number of cells results from the recruitment of different populations of multipotent mesenchymal stromal/stem cells (MSCs) residing in the tissues. We hypothesized that the impact of hygiene of housing conditions during growth on the proportions of MSCs in adipose tissue and skeletal muscle may differ between pigs with different residual feed intake (RFI), a measure of feed efficiency.MethodsAt the age of 12 weeks, Large White pigs from two lines divergently selected for low and high RFI were housed in two contrasting hygiene conditions (good vs poor). After six weeks, pigs were slaughtered (n = 30; 5-9/group). Samples of subcutaneous adipose tissue and longissimus skeletal muscle were collected, and cells from the stromal vascular fraction (SVF), which includes mesenchymal stromal/stem cell populations, were isolated from each tissue. Adipose and muscle cell populations from the SVF were phenotyped by flow cytometry using antibodies that targeted different cell surface markers (CD45 to separate hematopoietic cells from MSCs; CD34, CD38, CD56 and CD140a to identify MSC populations with adipogenic and/or myogenic potential).ResultsAdipose tissue and muscle shared some common MSC populations although MSC diversity was higher in muscle than in adipose tissue. In muscle, the CD45-CD56+CD34-CD140a+ and CD45-CD56+CD34+CD140a+ cell populations were abundant. Of these two cell populations, only the proportions of CD45-CD56+CD34+CD140a+ cells increased (P < 0.05) in pigs housed in poor hygiene as compared with pigs in good hygiene conditions. For the CD45-CD56-CD34- cell population, present in low proportion, there was an interaction between hygiene condition and genetic line (P < 0.05) with a decrease in low RFI pigs housed in poor hygiene conditions. In adipose tissue, the two abundant MSC populations were CD45-CD56-CD34- and CD45-CD56+CD34-. The proportion of CD45-CD56-CD34- cells increased (P < 0.05) whereas the proportion of CD45-CD56+CD34- tended to decrease (P < 0.1) in pigs housed in poor conditions. This study shows that the proportions of some MSC populations were affected by hygiene of housing conditions in a tissue-dependent manner in pigs of both RFI lines. It suggests that MSCs may play a significant role in adipose tissue and skeletal muscle homeostasis and may influence later growth and body composition in growing animals.
The wall-less bacterium Spiroplasma poulsonii builds a polymeric cytoskeleton composed of interacting MreB isoforms
Authors: Masson Florent , Pierrat Xavier , Lemaitre Bruno , Persat Alexandre 
Published: June 2021

AbstractA rigid cell wall defines the morphology of most bacteria. MreB, a bacterial homologue of actin, plays a major role in coordinating cell wall biogenesis and defining a cell’s shape. In contrast with most bacteria, the Mollicutes family is devoid of cell wall. As a consequence, many Mollicutes have undefined morphologies. Spiroplasma species are an exception as they robustly grow with a characteristic helical shape, but how they maintain their morphology remains unclear. Paradoxal to their lack of cell wall, the genome of Spiroplasma contains five homologues of MreB (SpMreBs). Since MreB is a homolog of actin and that short MreB filaments participate in its function, we hypothesize that SpMreBs form a polymeric cytoskeleton. Here, we investigate the function of SpMreB in forming a polymeric cytoskeleton by focusing on the Drosophila endosymbiont Spiroplasma poulsonii. We found that in vivo, Spiroplasma maintain a high concentration of all five MreB isoforms. By leveraging a heterologous expression system that bypasses the poor genetic tractability of Spiroplasma, we found that strong intracellular levels of SpMreb systematically produced polymeric filaments of various morphologies. Using co-immunoprecipitation and co-expression of fluorescent fusions, we characterized an interaction network between isoforms that regulate the filaments formation. Our results point to a sub-functionalization of each isoform which, when all combined in vivo, form a complex inner polymeric network that shapes the cell in a wall-independent manner. Our work therefore supports the hypothesis where MreB mechanically supports the cell membrane, thus forming a cytoskeleton.Significance statementBacteria shape is determined by their cell wall. The actin homologue MreB essentially determines shape by organizing cell wall synthesis at the subcellular level. Despite their lack of cell wall, Spiroplasma robustly grow into long helical bacteria. Surprisingly, its genome retains five copies of mreB while it lost genes encoding canonical MreB interactors. We sought to delineate the exact function of Spiroplasma MreBs (SpMreBs). We leveraged in vivo data along with functional studies to systematically investigate MreB polymerization behavior. We uncovered that SpMreBs build into filaments, which structure it determined by a complex interaction network between isoforms. Our results support the hypothesis that MreB can mechanically support the membrane of Spiroplasma, hence acting as a load-bearing cytoskeletal protein.
Contrast – a lightweight Python framework for beamline orchestration and data acquisition
Authors: Björling Alexander , Weninger Clemens , Kahnt Maik , Kalbfleisch Sebastian , Johansson Ulf , Sala Simone , Lenrick Filip , Thånell Karina 
Published: June 2021

The emergence of fourth-generation synchrotrons is prompting the development of new systems for experimental control and data acquisition. However, as general control systems are designed to cover a wide set of instruments and techniques, they tend to become large and complicated, at the cost of experimental flexibility. Here we present Contrast, a simple Python framework for interacting with beamline components, orchestrating experiments and managing data acquisition. The system is presented and demonstrated via its application at the NanoMAX beamline of the MAX IV Laboratory.
Public debt and economic growth: contemporary evidence from a developing economy
Authors: Hilton Sam Kris 
Published: June 2021

PurposeConsidering the continuous rise in the public debt stock of developing countries (particularly Ghana) with the unstable economic growth rate for the past decades and the recent borrowing because of the impact of COVID 19, this paper aims to examine the causal relationships between public debt and economic growth over time.Design/methodology/approachThe paper uses a dynamic multivariate autoregressive-distributed lag (ARDL)-based Granger-causality model to test the causal relationships between public debt and economic growth [gross domestic product (GDP)]. Annual time-series data that spanned 1978–2018 were sourced from the World Bank Development Indicator database and the IMF fiscal Affairs Department Database and WEO.FindingsThe results reveal that public debt has no causal relationship with GDP in the short-run but there is unidirectional Granger causality running from public debt to GDP in the long run. Again, investment spending has a negative bi-directional causal relationship with GDP in the short-run but they have a positive bi-directional causal relationship in the long run. Conversely, no short-run causal relationship exists between government consumption expenditure and GDP but long-run Granger causality runs from government consumption expenditure to GDP. Finally, public debt has a positive impact on the inflation rate in the short run.Practical implicationsThe findings imply that government(s) must ensure high fiscal discipline to serve as a precursor for the effective and efficient use of recent borrowing, that is, the loans should be used for highly prioritized projects (preferably investment spending) that are well evaluated and self-sustained to add positively to the GDP.Originality/valueThis paper provides contemporary findings to augment extant literature on public debt and economic growth by using variables and empirical models, which prior studies could not sufficiently cover in a developing country perspective and affirms that public debt contributes to GDP only in the long run.
Enhancing users involvement in architectural design using mobile augmented reality
Authors: Shouman Bassam , Othman Ayman Ahmed Ezzat , Marzouk Mohamed 
Published: June 2021

PurposeMobile augmented reality (MAR) is one of the advanced three-dimensional (3D) representation tools that has been recently utilized in the construction industry. This paper aims to assess a user’s involvement levels through MAR application that has been experimented against traditional involvement techniques through an existing facility, plan re-designing scenario.Design/methodology/approachThrough reviewing related literature studies in the MAR field, an application has been developed that can superimpose real design alternatives on paper-based markers, allowing for flexible wall positioning, interior and exterior wall material application. As such, an enhanced user involvement experience is created. To measure user involvement levels, the application is experimented with 33 participants having the British University in Egypt’s library building as a case study, followed by survey questionnaires to gather and evaluate user responses.FindingsThe results of the analyzed data using SPSS indicated that MAR showed a positive impact on enhancing user involvement and better understanding of design projects. It also allowed users to produce different design alternatives in comparison to the traditional involvement approaches where users showed low design interaction and understanding.Originality/valueThe interactive features of the proposed application facilitate implementing ideas in design of construction projects that require user involvement.
Impact of personal values on customer satisfaction and word of mouth for luxury hotel service consumption in Confucian culture: evidence from Vietnam
Authors: Cam Le Nguyen Thi , Quy Vo Thi , Kim Quy Hoang Thi 
Published: June 2021

Purpose
This research aims to investigate the effect of personal values on customer satisfaction and whether positive experiences lead to positive word of mouth (WOM) about the luxury hotel service from the Confucian perspective.

Design/methodology/approach
This research developed a structural equation model for testing. Research data collected from Confucian participants who have used luxury hotel services in Vietnam, where Confucianism is the dominant cultural paradigm.

Findings
The results show that there is a positive relationship between Confucian customer personal values and satisfaction, as well as between customer satisfaction and positive WOM; personal values had no effect on positive WOM.

Research limitations/implications
This research implies that the personal values of Confucian consumers constitute the lens through which they view the world, thus the service personal values reflect their priorities on luxury hotel service consumption. A key to success for luxury hotel service providers lies in providing services that enhance Confucian customer personal values, which allow service providers to intercommunicate with the Confucian consumers’ core.

Originality/value
This research is significant because there is little research on personal values from the cultural mechanism of Confucian culture. This research also comes up with a richer understanding of the relationship between customer personal values and behaviors within the luxury hotel segment.
Commitment profiles of nascent entrepreneurs: insights from an empirical taxonomy among French student entrepreneurs
Authors: Gabay-Mariani Laetitia , Boissin Jean-Pierre 
Published: June 2021

PurposeIn line with an emerging body of literature questioning student entrepreneurs’ practices, and recent calls to bridge the intention-action gap, this contribution aims to identify profiles of commitment among nascent entrepreneurs, and their relationship with the performance of entrepreneurial behaviors.Design/methodology/approachRelying on Meyer and Allen's multidimensional model, the authors build an empirical taxonomy regarding affective and instrumental forms of commitment experienced by nascent entrepreneurs (n = 328) operating within French higher education.FindingsThe authors identify three commitment profiles – weak, affective and total – associated with distinct levels of advancement and investment in the entrepreneurial process. This analysis leads them to map out the entrepreneurial process followed by nascent entrepreneurs with three main thresholds: the initial threshold, the resonance threshold and the irreversibility threshold.Research limitations/implicationsThe work contributes to an emerging field of research dedicated to student entrepreneurship. It highlights the existence of different trajectories among nascent entrepreneurs, but also to different ways of being tied to them. It also enriches more broadly the understanding of the entrepreneurial process, especially its volitional phase.Practical implicationsThe results are also important to guide public action, especially to design relevant support programs accounting for nascent entrepreneurs' diversity.Originality/valueThis is the first research to identify profiles of nascent student entrepreneurs based on the way they feel tied to their project, but also to the broader project of becoming entrepreneurs.
Initial teacher education students' perceptions during a practicum in primary schools: a New Zealand experience
Authors: Fletcher Jo , Astall Chris , Everatt John 
Published: June 2021

PurposeThis paper is about mentoring of initial teacher education (ITE) students whilst on their practicum.Design/methodology/approachInformed by a social constructivist theoretical framework, an online survey was used to capture the breadth of quantitative data and the richness of qualitative responses relating to factors that impact student teachers' experiences during practicum.FindingsQuantitative data indicate that many student teachers were positive about the practicum, but this varied across the type of school in which they were placed. The qualitative data analyses showed a greater in-depth understanding of the range of issues that impacted how student teachers are treated in their role as a mentee by the mentor and the wider school community.Practical implicationsBetter understanding the experiences of student teachers helps to inform ITE providers of the critical role that mentor teachers play in preparing student teachers. The practical implications are that strategies to develop deep and collaborative partnerships amongst ITE providers, mentor teachers and school leaders, which build stronger understandings of a mentor teacher's role, are critical in order to support student teachers.Originality/valueThis research study repositions the critical nature of effective mentoring of student teachers so that mentor teachers and ITE providers can be informed by the voices and lived realities of these student teachers. The mentoring relationship needs to be critically interrogated to provide a more even and supportive “playing field” for all student teachers.
CSR fit and organizational attractiveness for job applicants
Authors: Agnihotri Arpita , Bhattacharya Saurabh 
Published: June 2021

Purpose
The purpose of this paper is to explore a conceptual model on the influence of corporate social responsibility (CSR) “fit” on organizational attractiveness among job applicants under the boundary conditions of chief executive officer (CEO) activism and CSR credibility.

Design/methodology/approach
The study is based on archival research, largely reviewing extant in CSR and drawing propositions based on existing theory.

Findings
Paper draws propositions based on CSR fit, credibility and CEO activism. The paper asserts the influence of CSR fit on organizational attractiveness among job applicants. It further explains moderated mediating mechanism through which CSR fit influences organizational attractiveness among job applicants.

Originality/value
Recruitment has primarily explored the role of CSR in attracting potential job candidates. This paper presents the role of CSR “fit,” an aspect not explored before in recruitment. This paper also introduces the role of CEO activism in recruitment.
Intraday analysis of regulation change in microstructure: evidence from an emerging market
Authors: Kadioglu Eyup 
Published: June 2021

PurposeThis study investigates the impact of simultaneously replacing both midday single-price call auction and lunch break with multi-price continuous trading on intraday volatility–volume patterns as well as the intraday volatility–volume nexus.Design/methodology/approachThe analysis utilises 150 m tick-by-tick transaction data related to 333 stocks traded on Borsa Istanbul Equity Market covering a period of 2 months prior to and following the change. In addition to graphic comparisons, the study uses difference in mean tests, panel-fixed generalized least squares (GLS), panel-random GLS and random-effects linear models with AR(1) disturbance regression estimations.FindingsThe results show that intraday volatility and trading volume form an inverse J-shape and are positively correlated. It is observed that the implementation of the regulation change decreased intraday volatility and increased trading volume. Additionally, the results indicate a negative volatility–liquidity and a positive volume–liquidity relationship, supporting the mixture of distribution hypothesis.Research limitations/implicationsEnhanced market efficiency provides greater opportunity for investment and risk management. Investors can benefit from the findings on the intraday volatility–volume nexus, which is an indicator of informed trading, and regulatory authorities can use volume to oversight volatility.Originality/valueThis very rare regulation change of the simultaneous replacement of the lunch break and midday call auction with continuous trading is investigated in the context of intraday volume and volatility. This study also expands upon some important findings on the volume–volatility nexus for the Turkish Stock Market.
Tribo-performance analysis of HVOF sprayed 86WC-10Co4Cr & Ni-Cr2O3on AISI 316L steel using DOE-ANN methodology
Authors: Singh Jashanpreet 
Published: June 2021

PurposeThe purpose of this paper is to carry out erosion wear investigation on high-velocity oxy-fuel (HVOF)-deposited 86WC-10Co4Cr and synergistic Ni/Chromia powder (i.e. 80Ni-20Cr2O3) on AISI 316L.Design/methodology/approachDesign of experiments-artificial neural network (DOE-ANN) methodology was adopted to calculate the erosion wear. Taguchi’s orthogonal array L16 (42) was used to perform set-of-erosion experiments followed by lower-the-better rule. The artificial neural network (ANN) model is used on erosion wear data obtained from the experiments.FindingsExperimental results indicate that 86WC-10Co4Cr provided better erosion wear resistance as compared to Ni/Chromia. The erosion wear of 86WC-10Co4Cr and synergistic Ni/Chromia coatings increases with an increase in time duration, solid concentration and time. The magnitude of erosion generated by ashes was comparatively lower than sand. The arithmetic mean roughness (Ra) of finished AISI 316L, 86WC-10Co4Cr and Ni/Chromia coating was found as 0.46 ± 0.13, 6.50 ± 0.16 and 7.04 ± 0.23 µm, respectively. Surface microhardness of AISI 316L, 86WC-10Co4Cr and Ni/Chromia coating was found as 197 ± 18, 1,156 ± 18 and 1,021± 21 HV, respectively.Practical implicationsThe present results can be useful for estimation of erosion wear in slurry pumps used in mining industry for the conveying of sand and in thermal power plants for the conveying of ashes to the dyke area.Originality/valueThe erosion wear of HVOF-sprayed 86WC-10Co4Cr and Synergistic Ni/Chromia powders was studied experimentally as well as predicted by the ANN model, and wear mechanisms are well discussed by scanning electron micrographs.
The impact of empowerment-focused human resource management on relationship learning and innovation
Authors: Zhang Huiying , Yang Man , Huo Baofeng 
Published: June 2021

PurposeIn the field of innovation, there is growing interest in exploring the factors that determine the extent to which firms can learn from external sources. However, most previous studies neglect the role of human factors. Little is known about which employee behaviors are desirable for boundary-spanning learning activities and which human resource management (HRM) practices are appropriate to respond to external knowledge transfer across boundaries. To fill this gap, the authors investigate the role of empowerment-focused HRM in interfirm learning and explore the integration of external inputs from the perspective of employees.Design/methodology/approachBased on empirical survey data collected from different countries, the authors test the proposed model with structural equation modeling.FindingsThe authors’ findings indicate that empowerment-focused HRM practices, including job enrichment, job autonomy, teamwork and cross-functional communication, are positively associated with relationship learning.Originality/valueIn this study, the authors present a theoretical explanation for how empowerment-focused HRM may influence firm's innovation through relationship learning process and provide empirical evidence regarding the specific HRM practices that can have different effects on the different phases of relationship learning.
Adoption of sustainable agricultural practices in Brazil: understanding the influence of socioeconomic and psychological factors
Authors: Foguesatto Cristian Rogério , Machado João Armando Dessimon 
Published: June 2021

PurposeDespite substantial efforts made by Brazil’s government to increase the adoption rate of sustainable agricultural practices (SAP), many of them have not been adopted by the farmers. This paper explores the factors influencing the adoption of SAP in the state of Rio Grande do Sul, one of the largest Brazilian grain producers.Design/methodology/approachUsing four logistic models, the authors test a conceptual framework that provides a systemic view of farmers' adoption decisions from a sample of 172 farmers.FindingsThe findings show that the adoption of SAP is influenced by farmers' socioeconomic characteristics (e.g. gender, level of education, farm size, asset values and farm income), attributes of SAP (compatibility of agricultural practice) and psychological factors (ecocentrism, anthropocentrism, and beliefs about climate change).Research limitations/implicationsThe results presented may not reflect the characteristics of the region studied because random sampling was not employed. Also, other psychological statements should be used in the questionnaire.Originality/valueLittle attention has been given to the analysis of farmers' psychological factors in studies on the adoption of agricultural conservation practices. This study provides a “systemic approach” that measures both socioeconomic and psychological factors.