bluuebunny/crossref_metadata_2025_split huggingface dataset:
Continuous Improvement of Quality Management in the Fall Prevention Process for Inpatients: a Historically Controlled Study
Authors: XU Hua , Cao Xiang , ZHANG Yanhong , Chen Zhaohong 
Published: June 2021

Abstract
Background: Falls are one of the most common adverse nursing events and sensitive indicators to measure nursing quality. The interventions for fall prevention are important; however, monitoring tools for the implementation are scarce.Methods: To explore the effects of monitoring the fall prevention process in reducing inpatients’ falls. Historically controlled trial and retrospective analysis. Explore and refer to the "structure-process-result" quality evaluation model. The design included assessment of fall risks, knowledge of prevention, participation of patients and their families, environment and management. The monitoring form for the implementation of fall prevention measures was used to monitor the effect and reduce the incidence rate of falls. Results: The incidence rate of falls among inpatients with different risk levels was compared and analyzed before monitoring (control group) and 12 months, 24 months, 36 months later after monitoring. The trend chi-square results showed that the incidence rate of falls decreased from 0.199‰ to 0.101‰, and the difference was statistically significant (p < 0.05). The probability of falls 12 months and 36 months later after intervention was 0.84 times and 0.51 times that of control group. The proportion of patients at high risk of falls had a decreasing trend.Conclusions: After the use of the monitoring form, the incidence rate of falls reduces, nurses' professional quality and personal ability are improved, the awareness of patients and family members participating in patient’s safety is strengthened, the environmental safety management is strengthened and the hardware facility is improved.
Computer Vision Based Detection and Quantification of Extraneous Water in Raw Milk
Authors: Asefa Bezuayehu Gutema , Hagos Legesse , Kore Tamirat , Emire Shimelis Admassu 
Published: June 2021

Abstract
A rapid method based on digital image analysis and machine learning technique is proposed for the detection of milk adulteration with water. Several machine learning algorithms were compared, and SVM performed best with 89.48 % of total accuracy and 95.10 % precision. An increase in the classification performance was observed in extreme classes. Better quantitative determination of the extraneous water was achieved using SVMR with R2(CV) and R2(P) of 0.65 and 0.71 respectively. The proposed technique can be used to screen raw milk based on the level of added extraneous water without the necessity of any additional reagent.
Identification of Genes During the Reprogramming of Fibroblast using Bioinformatics Analysis
Authors: Wu Peng , Sai Xiyalatu , Tang Xiaoying , Guo Huiming , Huang Huanlei , Nasser Moussa Ide , Zhu Ping 
Published: June 2021

Abstract
Bioinformatics method was used to screen Human induced pluripotent stem cells reprogrammed by Human Fibroblasts (FBs). The reprogramming efficiency was explored by difference expressed genes (DEGs) before and after the expression of hiPSCs. Likewise, microarray datasets GSE34309, GSE43996, and GSE56805 datasets were downloaded from Gene Expression Omnibus (GEO) and GEO2R to screen the differentially expressed DEGs. Further, Gene Ontology (GO) and KEGG (Kyoto Encyclopedia of Genes and Genomes (KEGG) signaling pathways were analyzed by David database. Likewise, to explore the correlation between DEGs, and the STRING database software was used to construct the protein-protein internet (PPI), and the Cytoscape_v3.7.2 Cytohubba plug-in was used to screen out the core genes. A total of 622 DEGs were identified, including 344 up regulated genes and 278 down-regulated genes. DEGs were mainly concentrated in the cell cycle and PI3K-Akt signaling pathway. Among the ten core genes screened, CDK1, IL6, EGFR, FN1, CDH1, SOX2, BRCA1, EZH2, CD44, and CCNB1 were most significant in the reprogramming of FBs into iPSCs. Regardless underline the differential of those gene expression profiles and regulatory network during FBs reprogramming could provide a theoretical basis for efficient reprogramming.
Association Between the lncRNA TDRG1 rs8506 C&gt;T Polymorphism and Susceptibility To Kawasaki Disease in Chinese Children
Authors: Li Jinqing , Guo Kai , Yu Hongyan , Xu Yufen , Yang Chuanzi , Qiu Lijuan , Zhou Huazhong , Pi Lei , Che Di , Fu Lanyan , Wei Bing , Ma Shuxuan , Gu Xiaoqiong 
Published: June 2021

Abstract
Background

Kawasaki disease (KD) is an acute systemic vasculitis with unknown pathogen, and the formation of coronary artery lesions/aneurysm (CAL/CAA) is the most common sequela. Environmental and genetic factors have been suggested to be involved in the pathogenesis of KD. Human testis development related 1(TDRG1) is a newly identified long non-coding RNA (lncRNA) which stimulates the vascular endothelial growth factor (VEGF) pathway. The aim of this study was to investigate the association between genetic polymorphism of TDRG1 and the risk of KD.
Methods

A total of two cohorts from Guangzhou (988 KD patients and 1054 controls) and Beijing (564 KD patients and 1221 controls) were enrolled in the present study. Rs8506 of TDRG1 was chosen for TaqMan genotyping assay.
Results

Logistic regression suggested that lncRNA TDRG1 rs8506 C > T polymorphism was not associated with the risk of KD in both Guangzhou and Beijing cohort (dominant model and recessive model: P > 0.05). Moreover, we also did not observe a significant association between the lncRNA TDRG1 rs8506 C > T polymorphism and the risk of KD patients with different ages, gender or coronary artery outcomes in both Guangzhou and Beijing cohort.
Conclusions

The present study revealed that the lncRNA TDRG1 rs8506 C > T polymorphism might not be associated with susceptibility to KD in Chinese children. Future research with a larger sample size should be performed to confirm these results.
An iteratively optimised process for improving the efficiency and effectiveness of an industrial SARS-CoV-2 diagnostic facility
Authors: Douthwaite Julie A. , Brown Christopher A. , Ferdinand John R. , Sharma Rahul , Elliott Jane , Taylor Molly , Malintan Nancy T. , Duvoisin Hannah , Hill Thomas , Delpuech Oona , Orton Alexandra L. , Pitt Haidee , Kuenzi Fred , Fish Simon , Nicholls David J. , Cuthbert Anna , Richards Ian , Ratcliffe Giles , Upadhyay Abhishek , Marklew Abigail , Hewitt Craig , Ross-Thriepland Douglas , Brankin Christopher , Chodorge Matthieu , Browne Gareth , Mander Palwinder K. , DeWildt Ruud M. , Weaver Shane , Smee Penny A. , Kempen Joost van , Bartlett Jon G. , Allen Paula M. , Koppe Emma L. , Ashby Charlotte A. , Phipps Julian D. , Mehta Nalini , Brierley David J. , Tew David G. , Leveridge Melanie V. , Baddeley Stuart M. , Goodfellow Ian G. , Green Clive , Abell Chris , Neely Andy , Waddell Ian , Rees Steve , Maxwell Patrick H. , Pangalos Menelas N. , Howes Rob , Clark Roger 
Published: June 2021

Abstract
On 11th March 2020, the UK government announced plans for the scaling of COVID-19 testing, and on 27th March 2020 it was announced that a new alliance of private sector and academic collaborative laboratories were being created to generate the testing capacity required. The Cambridge COVID-19 Testing Centre (CCTC) was established during April 2020 through collaboration between AstraZeneca, GlaxoSmithKline, and the University of Cambridge, with Charles River Laboratories joining the collaboration at the end of July 2020. The CCTC lab operation focussed on the optimised use of automation, introduction of novel technologies and process modelling to enable a testing capacity of 22,000 tests per day. Here we describe the optimisation of the laboratory process through the continued exploitation of internal performance metrics, while introducing new technologies including the Heat Inactivation of clinical samples upon receipt into the laboratory and a Direct to PCR protocol that removed the requirement for the RNA extraction step. We anticipate that these methods will have value in driving continued efficiency and effectiveness within all large scale viral diagnostic testing laboratories.
Effect of Single-Port Inflatable Mediastinoscopy Simultaneous Laparoscopic-Assisted Radical Esophagectomy On Respiration and Circulation
Authors: Ye Haibo , Wang Xiaojin , Li Xiaojian , Gan Xiangfeng , Zhong Hongcheng , Wu Xiangwen , Cao Qingdong 
Published: June 2021

Abstract
Background and Purpose

We previously developed a new surgical method, namely, single-port inflatable mediastinoscopy simultaneous laparoscopic-assisted radical esophagectomy. The purpose of this study was to evaluate the effect of carbon dioxide inflation on respiration and circulation using this approach.
Methods

From April 2018 to October 2020, 105 patients underwent this novel surgical approach. The changes in respiratory and circulatory functions were reported when the mediastinal pressure and pneumoperitoneum pressure were 10 and 12mmHg, respectively. Data on blood loss, operative time, and postoperative complications were also collected.
Results

104 patients completed the operation successfully, except for 1 patient who was converted to thoracotomy because of intraoperative injury. During the operation, respectively, the heart rate, mean arterial pressure, central venous pressure, peak airway pressure, end-expiratory partial pressure of carbon dioxide and partial pressure of carbon dioxide increased in an admissibility range. The pH and oxygenation index decreased 1 hour after inflation, but these values were all within a safe and acceptable range and restored to the baseline level after CO2 elimination. Postoperative complications included anastomotic fistula (8.6%), pleural effusion that needed to be treated (8.6%), chylothorax (0.9%), pneumonia (7.6%), arrhythmia (3.8%) and postoperative hoarseness (18.2%). There were no cases of perioperative death.
Conclusions

When the inflation pressure in the mediastinum and abdomen was 10 mmHg and 12 mmHg, respectively, the inflation of carbon dioxide from single-port inflatable mediastinoscopy simultaneous laparoscopic-assisted radical esophagectomy did not cause serious changes in respiratory and circulatory function or increase perioperative complications.
Impact of Chronic Obstruction Pulmonary Disease with Acute Exacerbation on Survival in Current-Smoking Patients with Advanced stages Lung Squamous Cell Carcinoma undergoing Concurrent Chemoradiotherapy
Authors: Chiu Kuo-Chin , Lin Wei-Chun , Chang Chia-Lun , Wu Szu-Yuan 
Published: June 2021

Abstract
BACKGROUNDTo date, no data are available regarding the effect of chronic obstruction pulmonary disease (COPD) and COPD with acute exacerbation (COPDAE) on survival in patients with lung squamous cell carcinoma (SCC) receiving definitive concurrent chemoradiotherapy (CCRT).PATIENTS AND METHODSCurrent-smoking patients with clinical stage IIIA-IIIB, lung SCC who had received standard definitive CCRT and categorized them into two groups based on their COPD status to compare overall survival outcomes. We also examined the effects of COPD severity (0, 1, or ≥2 hospitalizations for COPDA within 1 year before CCRT). The aHRs (95% CIs) of lung cancer death for 1 and ≥2 hospitalizations for COPDAE within 1 year before CCRT were 1.21 (1.09, 1.39) and 1.63 (1.34, 1.97), respectively, compared with no hospitalization for COPDAE.RESULTSIn the inverse probability of treatment weighting (IPTW)-adjusted model, the adjusted hazard ratio (aHR) (95% confidence interval [CI]) of all-cause death for COPD was 1.06 (1.07, 1.71) compared with the non-COPD group. Moreover, in the IPTW-adjusted model, the aHRs (95% CIs) of 1 and ≥2 hospitalizations for COPDAE within 1 year before CCRT were 1.29 (1.16, 1.43) and 1.77 (1.41, 2.13), respectively, compared with no hospitalization for COPDAE.CONCLUSIONCOPD and its severity are significant independent risk factors for all-cause death in patients with stage IIIA-IIIB lung SCC receiving definitive CCRT. Hospitalization for COPDAE within 1 year before CCRT is the significant independent risk factors for lung cancer death in the patients with stages IIIA-IIIB lung SCC receiving definitive CCRT.
DRP1-Mediated Mitochondrial Fission Regulates the Lung Epithelial Response to Allergen
Authors: Bruno Sierra , Kumar Amit , Mark Zoe , Chandrasekaran Ravishankar , Nakada Emily , Chamberlain Nicolas , Mihavics Bethany , Walzer Joseph , Cahoon Jonathon , Dixon Anne E. , Cunniff Brian , Anathy Vikas 
Published: June 2021

Abstract

Background: Mitochondria regulate a myriad of cellular needs and functions. Dysregulation of mitochondrial control within airway epithelial cells has been implicated in the pro-inflammatory response to allergens in asthmatics. Because of their multifaceted nature, mitochondrial structure needs to be tightly regulated through fission and fusion. Dynamin Related Protein 1 (DRP1), a cytosolic GTPase, is a key driver of mitochondrial fission. During allergic asthma, airway epithelial mitochondria appear smaller and structurally altered. The role of DRP1-mediated mitochondrial fission, however, has not been fully elucidated in allergic airway disease. Methods: We used a Human Bronchial Epithelial Cell line (HBECs), primary Mouse Tracheal Epithelial Cells (MTECs), and conditional ablation of DRP1 in lung epithelial cells to investigate mitochondrial fission and its impact on the pro-inflammatory response to House Dust Mite (HDM) in vitro and in vivo. Results: Our data suggest that, following HDM challenge, mitochondrial fission is rapidly upregulated in airway epithelial cells and precedes production of pro-inflammatory cytokines and chemokines. Further, deletion of DRP1 in lung epithelial cells lead to decreased mitochondrial fission and enhanced pro-inflammatory signaling in response to HDM. Analysis of lung epithelial specific DRP1 deletion in mice demonstrated enhanced Airway Hyper Responsiveness (AHR), inflammation, differential mucin transcription, and epithelial cell death. Conclusions: Mitochondrial fission is rapidly upregulated in airway epithelial cells following HDM exposure, prior to epithelial release of pro-inflammatory cytokines and chemokines. Deletion of DRP1, a necessary pro- fission protein, reduces fission and enhances the pro-inflammatory epithelial response to HDM, exacerbating the allergic response.
In Vitro Radiosensitization by Eribulin in Human Cancer Cell Lines
Authors: Benlloch Raquel , Castejón Raquel , Rosado Silvia , Coronado María José , Sánchez Patricia , Romero Jesús 
Published: June 2021

Abstract
Objectives: To determine the radiosensitizing properties of eribulin and to establish the potential mechanisms of radiosensitization in cervical (HeLa) and pharyngeal (FaDu) cancer cell lines.Material and Methods: Cytotoxicity was evaluated by the crystal violet method. The 10% and 50% inhibitory concentration (IC10, IC50) for 24-hour drug exposure were determined in both cell lines. The surviving fraction at 2Gy (SF2) and the sensitizer enhancement ratio (SER) were calculated from radiation cell survival curves in presence or absence of eribulin. Combination index (CI) was calculated to determine if there is a true synergistic interaction between eribulin and irradiation. Cell cycle changes were assessed by propidium iodide staining and flow cytometry analysis. Apoptotic cells were detected by FITC-conjugated annexin-V labelling and flow cytometry and by immunofluorescence with TUNEL-assay.Results: Mean IC50s and IC10s were 1.58nM and 0.7nM and 0.7nM and 0.27nM for HeLa and FaDu cells respectively. Radiosensitization was observed in both lines tested with a SER up to 2.71 and 2.32 for HeLa and FaDu cells respectively. A true synergistic effect was showed with a CI of 0.82 and 0.76 for HeLa and FaDu cells respectively. Eribulin induced significant G2/M cell arrest and marked apoptosis. Irradiation combined with 3nM eribulin enhanced radiation induced apoptosis in Hela cells.Conclusions: Eribulin shows a true in vitro radiosensitizing effect in tested cell lines by inducing significant G2/M phase arrest and apoptosis changes. Further studies are needed to assess the clinical benefits of concurrent eribulin and radiotherapy as a novel therapeutic strategy for cancer.
Genomic Analysis of Lytic Phage V09 that Targets Pathogenic Vibrio Parahaemolyticus
Authors: Ni Peien , Lei Xiaoling , Yin Yujie , Almeida Adelaide , Wang Dapeng 
Published: June 2021

Abstract
Phage therapy is a promising approach to control foodborne pathogenic bacteria. In this study, a lytic phage against Vibrio parahaemolyticus, designated Vibrio phage V09, was isolated from surface water in Shanghai, China and its genomic DNA was sequenced. The data demonstrated that phage V09 is a double-stranded DNA phage. Its genome consists of 243,881 base pairs (42.6% guanine-cytosine content) encoding 377 open reading frames and with 27 tRNAs detected. According to phylogenetic analysis, phage V09 can be considered a member of the subfamily Tevenvirinae and family Myoviridae.
Cost-Utility Analysis of a Chronic Back Pain Multidisciplinary Biopsychosocial Rehabilitation (MBR) compared to Standard Care for Privately Insured in Germany
Authors: Hochheim Martin , Ramm Philipp , Wunderlich Max , Amelung Volker 
Published: June 2021

Abstract
Introduction: Multidisciplinary biopsychosocial rehabilitation (MBR) is highly recommended to treat chronic lower back pain (CLBP). However, its economic benefit remains to be clearly demonstrated. Objective: To analyse the effect of a 12-month MBR with behaviour-change coaching and device-supported exercise on direct medical costs, sick leave and health-related quality of life (HRQOL) at 24 months. Methods: A cohort of privately insured in Germany was evaluated using administrative and trial data. After removing dissimilarities in characteristics between MBR and control via propensity score matching, treatment effects were calculated with a Difference-in-Difference approach.Results: The base-case analysis comprised 112 MBR participants and 111 members in the standard care group. With an incremental cost-effectiveness ratio (ICER) of €8,428 per quality-adjusted life year (QALY) gained, the intervention was classified as cost-effective. Economically unaccounted sick leave due to back pain (BP) in the last six months was reduced by 17.5 days (p = 0.001) in the MBR group. Development of HRQOL was positive for the MBR (0.046, p= 0.026). Subgroup analysis with major impaired participants demonstrated the possibility of a dominant intervention with an ICER of - €6,861 per QALY. Savings were driven by a reduction in BP specific costs by - €1,733 (p= 0.035). Difference in sick leave was 27 days (p = 0.006) in favour of the MBR group.Conclusion: This is the first cost-utility study with combined data from a private health insurer and a controlled trial to demonstrate that long term MBR is cost-effective in the treatment of CLBP. Subgroups with major impairment benefit more from the intervention than participants with minor impairment. MBR significantly reduces sick leave in all participants making it a profitable intervention from society’s point of view.
Covid-19 pandemic in India: Chronological comparison of the regional heterogeneity in the pandemic progression and gaps in mitigation strategies
Authors: Datta Satabdi , Chakroborty Neloy Kumar , Sharda Deepinder , Attri Komal , Choudhury Diptiman 
Published: June 2021

Abstract
COVID-19 second wave, in India, exerted more catastrophe than the first. The progressions of both waves were heterogeneous in the six different regions involving 28 states and 8 union territories. An analysis of the temporal variations in new cases and fatalities in all the states of India was done for both the 1st (30th January 2020 to 31st January 2021) and 2nd wave (1st February 2021 to 29th May 2021) showed India accounted for over 16% and 9% of the cases and fatalities of the world respectively. The Southern and Western regions remained the top contributor of cases and fatalities in both waves. The state of Punjab and Maharashtra reported the highest CFR (3.24 and 2.5 respectively) in the country during 1st wave, and in the second wave, Andaman & Nicobar Islands (2.6), and Punjab (2.25) reported the highest CFR. Goa and Delhi showed the highest CCR and CDR for 1st wave respectively, whereas Lakshadweep and Goa reported the highest CCR and CDR respectively in the 2nd wave. The study comprehends the severity of the second wave over all the states of the country, highlights major hotspots regions and some gaps in mitigation strategies.
Dynamics of Small World Effect and Random Fuzzy Graph
Authors: Zhang Yijin , Huang Jie , Lin Zongbing 
Published: June 2021

Abstract
Small World Effect (or Six Degrees of Separation theory) has generated significant influence in the world. Many researchers and institutes have done lots of work on the study of it. We model Small World Effect by random process and Graph, calculate the probability that any two people i and j in the world can contact each other after n steps (forwarding messages by intermediaries) based on different R (average number of acquaintances everyone has in the world).When R=50 or 80, if n≥5, the probability is 0.848 and the search is very likely to happen. When R=150, n=4, this probability is 0.99994, that is, after 4 steps, any two people in the world will establish connection almost surely. In the sense of Dunbar's number (R=150), six degrees of separation becomes four degrees of separation. We propose the concepts of (directed) Random Fuzzy Graph for the very first time which can describe the fact of recognition among people best, because the relation among persons is random and fuzzy.