bluuebunny/crossref_metadata_2025_split huggingface dataset:
Technological entrepreneurship: present conditions and future perspectives for Latin America
Authors: Yáñez-Valdés Claudia 
Published: November 2021

Purpose
Technological entrepreneurship is distinguished from other types of initiatives by its innovative characteristics and rapid growth, as well as by the high uncertainty it faces, both in terms of investment cost and capacity for continuous adaptation. The purpose of this study is to investigate the definitions, measures and methods that have been used to investigate the phenomenon and to define the process of the “entrepreneurial journey” and the associated typologies according to the technological level.

Design/methodology/approach
This paper provides a systematic review of the literature on technological entrepreneurship focusing on emerging economies in Latin America. Based on the review of 62 papers, it analyses the current state and future research perspectives on the topic.

Findings
The authors can conclude that technological entrepreneurship in Latin America has great opportunities, especially linked to growth, but faces the threats of an uncertain and dynamic environment. Taking advantage of technologies allows the formulation of unique value propositions linked to the environment through innovation that improves the quality of life of the population.

Research limitations/implications
This study is a starting point for the development of research on resilient technology ventures. Latin America represents a limited location but offers insight into the developing phenomenon to measure the impacts that this phenomenon generates in society.

Originality/value
Considering the possibilities offered by technology and its continuous evolution, the impact that this type of entrepreneurship generates on the environment can be on a global scale. However, high growth and success are as likely as a failure and this is part of the improvement process.
Does environmental proactivity make a difference? The critical roles of green operations and collaboration in GSCM
Authors: Kitsis Aleksandr M. , Chen Injazz J. 
Published: November 2021

PurposeThis paper aims to examine the complex links between environmental proactivity, collaboration with suppliers and customers, green operations and firm performance.Design/methodology/approachBased on a sample of 208 US companies, five main effects and two mediation effects were tested using structural equation modeling analysis.FindingsThis study reveals that environmental proactivity exerts positive effects on green operations and that collaboration mediates the above relationship. Further, green operations are a powerful driver of a firm’s economic and environmental performance. Findings also demonstrate the critical mediating role of green operations in linking collaboration with performance.Research limitations/implicationsThis research contributes to a scholarly debate by offering novel insights into the extent to which proactivity improves firm performance may be influenced by multiple supply chain practices. To managers, this study highlights the strategic value of environmental proactivity as it fosters collaboration and green operations in boosting a firm’s environmental and economic performance.Originality/valueThis study addresses a gap in the literature by investigating the links between environmental proactivity, collaboration, green operations and corporate performance.
Outcomes to measure the effects of pharmacological interventions for pain management for women during labour and birth: a review of systematic reviews and randomised trials
Authors: Tan A , Wilson AN , Eghrari D , Clark H , Tse WC , Bohren MA , Homer CSE , Vogel JP 
Published: November 2021

BackgroundPharmacological pain management options can relieve women’s pain during labour and birth. Trials of these interventions have used a wide variety of outcomes, complicating meaningful comparisons of their effects. To facilitate better assessment of the effectiveness of labour pain management in trials and meta‐analyses, consensus about key outcomes and the development of a core outcome set is essential.ObjectiveTo identify all outcomes used in studies of pharmacological pain management interventions during labour and birth.DesignA review of systematic reviews and their included randomised controlled trials was undertaken.Search strategyCochrane CENTRAL was searched to identify all Cochrane systematic reviews describing pharmacological pain management options for labour and birth. Search terms included ‘pain management’, ‘labour’ and variants, with no limits on year of publication or language.Selection criteriaCochrane reviews and randomised controlled trials contained within these reviews were included, provided they compared a pharmacological intervention with other pain management options, placebo or no treatment.Data collection and analysisAll outcomes reported by reviews or trials were extracted and tabulated, with frequencies of individual outcomes reported.Main resultsNine Cochrane reviews and 227 unique trials were included. In total, 146 unique outcomes were identified and categorised into maternal, fetal, neonatal, child, health service, provider’s perspective or economic outcome domains.ConclusionsOutcomes of pharmacological pain management interventions during labour and birth vary widely between trials. The standardisation of trial outcomes would permit the assessment of meta‐analyses for best clinical practice.Tweetable abstractOutcomes to measure pharmacological pain management options during labour are highly variable and require standardisation.
Contributions of side effects to contraceptive discontinuation and method switch among Kenyan women: a prospective cohort study
Authors: Rothschild CW , Richardson BA , Guthrie BL , Kithao P , Omurwa T , Mukabi J , Callegari LS , Lokken EL , John‐Stewart G , Unger JA , Kinuthia J , Drake AL 
Published: November 2021

ObjectiveTo determine the contribution of specific contraceptive side effects to method switch and modern‐method discontinuation among Kenyan women.DesignA prospective cohort study.SettingFive counties in Western Kenya.ParticipantsWomen aged ≥18 years old and emancipated female minors ≥14 years old using modern, reversible contraception were recruited while attending 10 public health facilities.MethodsPatient‐reported adverse effect symptoms, method switch and discontinuation were reported through weekly text message‐based surveys for 24 weeks.Main outcome measurementsPrevalence, hazards ratio (HR).ResultsAmong 825 women, 44% were using implants, 43% injectables, 7% an intrauterine device and 6% oral contraceptive pills at enrolment. Most (61%) women were continuing a method used in the previous month. During the 24‐week follow up, incidence of contraceptive switch was 61.3 per 100 person‐years (95% confidence interval [CI] 52.4–71.8) and incidence of discontinuation was 38.5 per 100 person‐years (95% CI 31.6–47.0). On average, one‐quarter (prevalence [Pr] 0.24, 95% CI 0.22–0.26) of participants reported side effects or method problems weekly, with sexual side effects the most prevalent symptom (Pr 0.15, 95% CI 0.13–0.16). Lack of expected bleeding was associated with higher risk of method switch (adjusted hazard ratio [aHR] 2.36, 95% CI 1.22–4.57). Risk of all‐modern method discontinuation was higher among women experiencing irregular bleeding (aHR 2.39, 95% CI 1.20–4.77), weight changes (aHR 2.72, 95% CI 1.47–4.68) and sexual side effects (aHR 2.42, 95% CI 1.40–4.20).ConclusionsAddressing irregular bleeding, weight changes and sexual side effects through development of new products that minimise these specific side effects and anticipatory counseling may reduce method‐related discontinuation.Tweetable abstractBleeding, weight changes, sexual problems associated with discontinuation of #contraception, but many continue despite side effects.
Phylogenomic analyses of the genusDrosophilareveals genomic signals of climate adaptation
Authors: Li Fang , Rane Rahul V. , Luria Victor , Xiong Zijun , Chen Jiawei , Li Zimai , Catullo Renee A. , Griffin Philippa C. , Schiffer Michele , Pearce Stephen , Lee Siu Fai , McElroy Kerensa , Stocker Ann , Shirriffs Jennifer , Cockerell Fiona , Coppin Chris , Sgrò Carla M. , Karger Amir , Cain John W. , Weber Jessica A. , Santpere Gabriel , Kirschner Marc W. , Hoffmann Ary A. , Oakeshott John G. , Zhang Guojie 
Published: November 2021

AbstractManyDrosophilaspecies differ widely in their distributions and climate niches, making them excellent subjects for evolutionary genomic studies. Here, we have developed a database of high‐quality assemblies for 46Drosophilaspecies and one closely relatedZaprionus. Fifteen of the genomes were newly sequenced, and 20 were improved with additional sequencing. New or improved annotations were generated for all 47 species, assisted by new transcriptomes for 19. Phylogenomic analyses of these data resolved several previously ambiguous relationships, especially in themelanogasterspecies group. However, it also revealed significant phylogenetic incongruence among genes, mainly in the form of incomplete lineage sorting in the subgenusSophophorabut also including asymmetric introgression in the subgenusDrosophila. Using the phylogeny as a framework and taking into account these incongruences, we then screened the data for genome‐wide signals of adaptation to different climatic niches. First, phylostratigraphy revealed relatively high rates of recent novel gene gain in three temperatepseudoobscuraand five desert‐adapted cactophilicmullerisubgroup species. Second, we found differing ratios of nonsynonymous to synonymous substitutions in several hundred orthologues between climate generalists and specialists, with trends for significantly higher ratios for those in tropical and lower ratios for those in temperate‐continental specialists respectively than those in the climate generalists. Finally, resequencing natural populations of 13 species revealed tropics‐restricted species generally had smaller population sizes, lower genome diversity and more deleterious mutations than the more widespread species. We conclude that adaptation to different climates in the genusDrosophilahas been associated with large‐scale and multifaceted genomic changes.
The behavioral economics of alcohol demand in Greek‐affiliated college students
Authors: Ortelli Olivia A. , Martinetti Margaret P. 
Published: November 2021

AbstractBackgroundCollege students affiliated with fraternity and sorority, or “Greek” life represent a known high‐risk group for alcohol consumption and related consequences, but little is known about demand for alcohol in this population. The current study examined behavioral economic demand for alcohol in a sample of Greek life‐affiliated undergraduate students using the alcohol purchase task (APT) and a novel variation of the APT that included a fixed‐price, nonalcoholic alternative (APT Choice).MethodsParticipants (n = 229) completed the APT, APT Choice, Alcohol Use Disorders Identification Test (AUDIT), and Daily Drinking Questionnaire (DDQ). Group demand indices were calculated for the entire sample and then separately for participants who met or did not meet the legal drinking age (21+ or underage, respectively). Independent‐sample t tests assessed whether there were any significant differences between the two age cohorts in the percent change in each behavioral economic index from the APT to APT Choice. Tests of correlation evaluated the construct validity of the demand indices from both hypothetical purchase tasks.ResultsDescriptive statistics on alcohol use in this Greek‐affiliated sample revealed “hazardous” drinking scores, with AUDIT‐C scores exceeding the threshold of alcohol misuse. These measures were significantly correlated with demand indices from both APT conditions, and demand was inversely related to price; however, demand for alcohol was reduced when a nonalcoholic alternative was available. Both age cohorts reported a reduction in BP1 (highest price of nonzero consumption) and an increase in α (rate of change in elasticity), but these changes were significantly greater among underage participants.ConclusionsAlthough Greek life‐affiliated students demonstrate high demand for alcohol, the concurrent availability of a nonalcoholic alternative reduces alcohol demand, particularly for underage students. These findings suggest that nonalcoholic options may enhance the effectiveness of increasing alcohol prices to reduce alcohol consumption among students at higher risk for alcohol use.
Chloride intracellular channel 4 (CLIC4) expression profile in the mouse medial prefrontal cortex and its regulation by ethanol
Authors: Bogenpohl James W. , Weston Rory M. , Foreman Taylor N. , Kitchen Kaitlyn E. , Miles Michael F. 
Published: November 2021

AbstractBackgroundChloride intracellular channel 4 (CLIC4) is a multifunctional metamorphic protein for which a growing body of evidence supports a major role in the brain's molecular and behavioral responses to ethanol (EtOH). Although key to understanding the functional biology underlying this role, little is known about the cellular and subcellular expression patterns of CLIC4 in brain and how they are affected by EtOH.MethodsWe used qRT‐PCR to assess Clic4 mRNA expression in the medial prefrontal cortex (mPFC) of C57BL/6J mice in the absence and presence of acute EtOH exposure. Two complementary immunohistochemical techniques were employed to assess the subcellular localization of the CLIC4 protein and its pattern of expression across brain cell types in the mPFC in the absence and presence of acute EtOH.ResultsThrough immunohistochemical and stereological techniques, we show that CLIC4 protein is robustly expressed by oligodendrocytes (most abundant), microglia, and astrocytes, with minimal expression in neurons. Following acute EtOH exposure, we observed a rapid increase in Clic4 mRNA expression in female but not male mice and an overall increase in the number of oligodendrocytes and astrocytes expressing the CLIC4 protein.ConclusionsThese findings suggest that Clic4 functions as an early response gene for acute EtOH in brain, which likely underlies its ability to modulate EtOH behavior. Our results also suggest that the role of CLIC4 in the brain's response to EtOH is mediated through oligodendrocytes.
Understanding uncontrolled severe allergic asthma by integration of omic and clinical data
Authors: Delgado‐Dolset María Isabel , Obeso David , Rodríguez‐Coira Juan , Tarin Carlos , Tan Ge , Cumplido José A. , Cabrera Ana , Angulo Santiago , Barbas Coral , Sokolowska Milena , Barber Domingo , Carrillo Teresa , Villaseñor Alma , Escribese María M. 
Published: November 2021

AbstractBackgroundAsthma is a complex, multifactorial disease often linked with sensitization to house dust mites (HDM). There is a subset of patients that does not respond to available treatments, who present a higher number of exacerbations and a worse quality of life. To understand the mechanisms of poor asthma control and disease severity, we aim to elucidate the metabolic and immunologic routes underlying this specific phenotype and the associated clinical features.MethodsEighty‐seven patients with a clinical history of asthma were recruited and stratified in 4 groups according to their response to treatment: corticosteroid‐controlled (ICS), immunotherapy‐controlled (IT), biologicals‐controlled (BIO) or uncontrolled (UC). Serum samples were analysed by metabolomics and proteomics; and classifiers were built using machine‐learning algorithms.ResultsMetabolomic analysis showed that ICS and UC groups cluster separately from one another and display the highest number of significantly different metabolites among all comparisons. Metabolite identification and pathway enrichment analysis highlighted increased levels of lysophospholipids related to inflammatory pathways in the UC patients. Likewise, 8 proteins were either upregulated (CCL13, ARG1, IL15 and TNFRSF12A) or downregulated (sCD4, CCL19 and IFNγ) in UC patients compared to ICS, suggesting a significant activation of T cells in these patients. Finally, the machine‐learning model built including metabolomic and clinical data was able to classify the patients with an 87.5% accuracy.ConclusionsUC patients display a unique fingerprint characterized by inflammatory‐related metabolites and proteins, suggesting a pro‐inflammatory environment. Moreover, the integration of clinical and experimental data led to a deeper understanding of the mechanisms underlying UC phenotype.
A prehabilitation programme implemented before robot‐assisted radical prostatectomy improves peri‐operative outcomes and continence recovery
Authors: Rahota Razvan‐George , Salin Ambroise , Gautier Jean‐Romain , Almeras Christophe , Garnault Valérie , Tollon Christophe , Loison Guillaume , Beauval Jean‐Baptiste , Ploussard Guillaume 
Published: December 2021

ObjectivesTo assess the impact of a routine, on‐site, 1‐day prehabilitation (PreHab) programme on peri‐operative and continence recovery after robot‐assisted radical prostatectomy (RARP).Materials and MethodsAll 303 consecutive RARPs performed between March 2018 and February 2020 since the routine implementation of PreHab were included in our study. PreHab was carried out according to the availability of the 1‐day programme before the planned date of surgery (two sessions per month including four patients per session). The PreHab programme was implemented in 165 patients (54.5%). The primary endpoint was continence recovery, strictly defined as no safety pad use at 1 and 6 months. Secondary endpoints were peri‐operative variables (blood loss, operating time, length of stay, transfusion, complications, and readmission rates). Comparisons were made according to whether the PreHab pathway was applied or not (PreHab+ vs PreHab−) in univariable and multivariable models.ResultsThe PreHab pathway was implemented for a stable proportion of patients over time (54.5%). The two cohorts were comparable in terms of preoperative and pathological features (P > 0.05). Length of stay was significantly shorter in the PreHab+ group (1.3 vs 1.9 days; P = 0.001). There was a trend towards fewer complications in the PreHab+ group (P = 0.061). Use of the PreHab pathway was independently correlated with higher continence rates at 1 month (37% vs 60%; P < 0.001) and 6 months (67.4% vs 87.3%; P < 0.001), even after controlling for age, body mass index, prostate volume, type of apical reconstruction, nerve‐sparing surgery and lymph node dissection. The main limitation of the study was the absence of randomization.ConclusionsOur experience demonstrates that the PreHab programme is the major predictor of improved peri‐operative outcomes and continence recovery after RARP, with sustainable benefits 6 months after surgery.
E239K mutation abolishes the suppressive effects of lysine‐specific demethylase 1 on migration and invasion of MCF7 cells
Authors: Zhang Yu , Wu Tong , Zhao Bo , Liu Ziyu , Qian Rui , Zhang Jing , Shi Yueru , Wan Youzhong , Li Zhe , Hu Xin 
Published: November 2021

AbstractLysine‐specific demethylase 1 (LSD1) is an important histone demethylase that mediates epithelial to mesenchymal transition (EMT). The E239K mutation of LSD1 was identified in a luminal breast cancer patient from the COSMIC Breast Cancer dataset. To investigate the functional effects of the E239K mutation of LSD1, a stable LSD1 knockdown MCF7 cell line was generated. Rescue with WT LSD1, but not E239K mutated LSD1, suppressed the invasion and migration of the LSD1 knockdown cells, indicating that the E239K mutation abolished the suppressive effects of LSD1 on the invasion and migration of MCF7 cells. Further analysis showed that the E239K mutation abolished LSD1‐mediated invasion and migration of MCF7 cells through downregulation of estrogen receptor α (ERα). Most importantly, the E239K mutation disrupted the interaction between LSD1 and GATA3, which reduced the enrichment of LSD1 at the promoter region of the ERα gene; the reduced enrichment of LSD1 at the promoter region of the ERα gene caused enhanced histone H3K9 methylation, which subsequently suppressed the transcription of the ERα gene. In summary, the E239K mutation abolishes the suppressive function of LSD1 on migration and invasion of breast cancer cells by disrupting the interaction between LSD1 and GATA3.
Eribulin prolongs survival in an orthotopic xenograft mouse model of malignant meningioma
Authors: Nakano Tomoyuki , Fujimoto Kenji , Tomiyama Arata , Takahashi Masamichi , Achiha Takamune , Arita Hideyuki , Kawauchi Daisuke , Yasukawa Mami , Masutomi Kenkichi , Kondo Akihide , Narita Yoshitaka , Maehara Taketoshi , Ichimura Koichi 
Published: November 2021

AbstractMeningioma is the most common intracranial tumor, with generally favorable patient prognosis. However, patients with malignant meningioma typically experience recurrence, undergo multiple surgical resections, and ultimately have a poor prognosis. Thus far, effective chemotherapy for malignant meningiomas has not been established. We recently reported the efficacy of eribulin (Halaven) for glioblastoma with a telomerase reverse transcriptase (TERT) promoter mutation. This study investigated the anti–tumor effect of eribulin against TERT promoter mutation‐harboring human malignant meningioma cell lines in vitro and in vivo. Two meningioma cell lines, IOMM‐Lee and HKBMM, were used in this study. The strong inhibition of cell proliferation by eribulin via cell cycle arrest was demonstrated through viability assay and flow cytometry. Apoptotic cell death in malignant meningioma cell lines was determined through vital dye assay and immunoblotting. Moreover, a wound healing assay revealed the suppression of tumor cell migration after eribulin exposure. Intraperitoneal administration of eribulin significantly prolonged the survival of orthotopic xenograft mouse models of both malignant meningioma cell lines implanted in the subdural space (P < .0001). Immunohistochemistry confirmed apoptosis in brain tumor tissue treated with eribulin. Overall, these results suggest that eribulin is a potential therapeutic agent for malignant meningiomas.
SUMOylation regulates Rb hyperphosphorylation and inactivation in uveal melanoma
Authors: Meng Fengxi , Yuan Yiqun , Ren Hui , Yue Han , Xu Binbin , Qian Jiang 
Published: November 2021

AbstractSmall ubiquitin‐like modifier (SUMO)ylation is one of the posttranslational modifications and is implicated in many tumor types. Modulation of SUMOylation can affect tumor progression, but the underlying mechanisms remain unclear. Here, we show that, for the first time, in uveal melanoma (UM), the most common intraocular malignancy in adults, global SUMOylation is upregulated and participates in tumor growth. Inhibition of SUMOylation in UM is sufficient to reduce tumor growth both in vitro and in vivo. Furthermore, we found that retinoblastoma protein (Rb) is a target protein and a critical downstream effector of the upregulated SUMOylation activity in UM. Increased SUMOylation of the Rb protein leads to its hyperphosphorylation and inactivation in UM cells, promoting UM cell proliferation. In summary, our results provide novel insight into the mechanism underlying SUMOylation‐regulated tumor growth in UM.