bluuebunny/crossref_metadata_2025_split huggingface dataset:
Toosendanin Inhibits Colorectal Cancer Cell Growth Through the Hedgehog Pathway by Targeting SHH
Authors: Zhang Meng , Tao Zhongyi , Gao Lijuan , Chen Fengyang , Ye Yiping , Xu Shifang , Huang Wenkang , Li Xiaoyu 
Published: December 2021

Abstract
Colorectal cancer (CRC) is one of the most common gastrointestinal cancers worldwide. It is complex and often fatal and is associated with a high disease-related mortality. The Hedgehog (Hh) signalling pathway plays indispensable roles in CRC. Many studies have proven that Shh is overexpressed in cancer stem cells (CSCs) and shown that SHH overexpression is positively correlated with CRC tumorigenesis. The development of new drugs to kill CRC cells through the Hh pathway is urgently needed. Toosendanin (TSN), a natural triterpenoid saponin extracted from the bark or fruit of Melia toosendan Sieb. et Zucc., has been proven to inhibit various tumours. Here, we demonstrated that TSN inhibited the CRC cell growth through the Hh signalling pathway by targeting SHH. TSN has promising potential as an antitumour agent for CRC treatment.
CENPF Upregulation is Associated with Immunosuppressive Status and Predicts Poor Clinical Outcomes in Lung Adenocarcinoma Validated by Qrt- PCR
Authors: xu zhiyun , Wang Shi , Ren Zhijian , Gao Xiang , Xu Lin , Ren Binhui , Zhang Shuai 
Published: December 2021

Abstract
Background: CENPF was differentially expressed in various cancers. However, the relationship between CENPF and immune infiltrates in lung adenocarcinoma was previously unknown.Methods: We implemented a comprehensive analysis of expression of CENPF in the GEO and TCGA databases. CENPF was evaluated for its prognostic value combining clinical samples from the GEPIA2 and TCGA databases. The Metascape together with WebGestalt databases were used for enrichment analysis of genesets that were most postively associated with CENPF. We retrieved the score for immune cell infiltration in TCGA data and examined the correlation between CENPF expression and the infiltration of immune cell by R software.Results: The outcomes exhibited that up-regulated CENPF mRNA expression was evidently related to poor PFS, DSS and OS in patients with lung adenocarcinoma. Moreover, high expression of CENPF was markedly related to genes associated with immune checkpoint. Further analysis showed that T cell CD4+ Th2 infiltration increased in lung adenocarcinoma samples with high CENPF expression.Conclusions: Our study indicated that CENPF led to T cell CD4+ Th2 infiltration through oncogenic activity and may be employed as a biomarker for the prediction of prognosis in lung adenocarcinoma.
Lungworms (Metastrongylus Spp.) Demonstrated in Domestic Pigs with Respiratory Disease - What was the True Relevance of that?
Authors: Wallgren Per , Pettersson Emelie 
Published: December 2021

Abstract
BackgroundAn outdoor pig herd was affected by severe respiratory disease in one out of three pastures. At necropsy, Mycoplasma hyopneumoniae and Pasteurella multocida were detected in the lungs, as well as the lung worm Metastrongylus apri. The life cycle of Metastrongylus spp. includes earth worms as an intermediate host, and since domesticated pigs mainly are reared indoors lungworms has not been diagnosed in domestic pigs in Sweden for decades, not even in pigs reared outdoors. Therefore, this disease outbreak was scrutinised from the view of validating the impact of Metastrongylus spp..ResultsAt the time of the disease outbreak, neither eggs of Metastrongylus spp. nor Ascaris suum were detected in faeces of pigs aged ten weeks. In contrast, five-months-old pigs at the pasture with respiratory disease shed large amounts of eggs from Ascaris suum, whereas Ascaris suum not was demonstrated in healthy pigs aged six months at another pasture. Low numbers of eggs from Metastrongylus spp. were seen in faecal samples from both these age categories.At slaughter, seven weeks later, ten normal weighted pigs in the preceding healthy batch were compared with ten normal weighted and five small pigs from the affected batch. Healing Mycoplasma-like pneumonic lesions were seen in all groups. Small pigs had more white spot liver lesions, and all small pigs shed eggs of Ascaris suum in faeces, compared to around 50% of the pigs in the normally sized groups. Metastrongylus spp. were demonstrated in 13 of the 25 pigs (52%), %), representing all groups included.ConclusionAs Metastrongylus spp. were demonstrated regardless of health status, and in another healthy outdoor herd, the impact of Metastrongylus spp. on the outbreak of respiratory disease was depreciated. Instead, Metastrongylus spp. was suggested to be common in outdoor production, although rarely diagnosed. The reason for this is because they will escape detection at routine inspection at slaughterhouses, and that they appeared to generally not induce clinical signs of respiratory disease. Instead, a possible association with a high burden of Ascaris suum was suggested to have preceded the severe outbreak with respiratory disease.
Multiscale 3D Genome Reorganization during Skeletal Muscle Stem Cell Lineage Progression and Muscle Aging
Authors: WANG Huating , Zhao Yu , Ding Yingzhe , He Liangqiang , Li Yuying , Chen Xiaona , Sun Hao 
Published: December 2021

Abstract
3D genome rewiring is known to influence spatiotemporal expression of lineage-specific genes and cell fate transition during stem cell differentiation and aging processes. Yet it is unknown how 3D architecture remodels and orchestrates transcriptional changes during skeletal muscle stem cell (also called satellite cell, SC) activation, proliferation and differentiation course. Here, using in situ Hi-C we comprehensively map the 3D genome topology reorganization at multiscale levels during mouse SC lineage progression and integrate with transcriptional and chromatin signatures to elucidate how 3D genome rewiring dictates gene expression program. Specifically, rewiring at compartment level is most pronounced when SC becomes activated. Striking loss in TAD border insulation and chromatin looping also occurs during early activation process. Meanwhile, TADs can also form TAD clusters and super-enhancer containing TAD clusters orchestrate stage-specific gene expression during SC early activation. Furthermore, we elucidate 3D chromatin regulation of key transcription factor, PAX7 and identify cis-regulatory elements that are crucial for local chromatin architecture and Pax7 expression. Lastly, 3D genome remodeling is profiled in SCs isolated from naturally aging mice, unveiling that geriatric SCs display a prominent gain in long-range contacts and loss of TAD border insulation. Genome compartmentalization and chromatin looping are evidently altered in aged SC while geriatric SC display a more prominent loss in strength of TAD borders. Together, our results implicate 3D chromatin extensively reorganizes at multiple architectural levels and underpin the transcriptome remodeling during SC lineage development and SC aging.
Super Strong Wide TM Mie Bandgaps Tolerating Disorders
Authors: Goudarzi Kiyanoush , Lee Moonjoo 
Published: December 2021

Abstract
This study demonstrates the appearance of super intense and wide Mie bandgaps in metamaterials composed of germanium rods in air that tolerate some disordering of rod position and rod radius under transverse magnetic (TM) polarized light waves. Results for Mie bandgap modes TM01 and TM11 tolerate rod-position disordering of 50%, and rod-radius disordering of 34 and 20%, respectively. Using these characteristics of TM11 under position and radius disordering, ultra-narrow straight, L-shaped and crossing waveguides that contain 14, four, and two rows of Ge rods in air are designed. Also, it is shown that TE01 Mie bandgap appear in metamaterials contain high refractive index, and disappear in metamaterials with lower refractive index such as silicon; in contrast, a new phenomenon of intense and broadband TM01, TM11, and TM21 in metamaterials with lower refractive index such as silicon appear. Also, in Si-based metamaterials, TM01 tolerates high rod-position and rod-radius disordering of 50% and 34%, respectively, and TM11 shows robustness to rod-position and rod-radius of 20%. This strong tolerance of disordering of TM modes in silicon and germanium metamaterials opens a new way to design small, high-efficient, and easy-fabricable optical devices for optical integrated circuits.
Psychological Distress And Smoking Behaviors of Chinese College Students: Mediating Effects of The Dimensions of Learning Burnout
Authors: Li Xiong , Tan Yuhua , Li Shanqing , Wang Xiaoxin 
Published: December 2021

Abstract
Objectives: Smokers or never smokers exposed to environmental tobacco use (ETS) are usually associated with various diseases and cancers. In order to better help college students prevent the tobacco use and thus preclude the incidences of avoidable diseases, this study explored the predictive power of different variables including demographic and psychological variables in relation to smoking behaviors. Methods: Maslach Burnout Inventory-Student Survey (MBI-SS) and Kessler Psychological Distress Scale (K10) were used in this study. Results: The multivariate logistic regression indicated that college students who were male (versus female, OR = 9.55), majoring in medicine and sports (versus nursing, ORmedicine = 2.19, ORsports=2.81), born in the non-singleton family (versus singleton family, OR= 0.63) with higher family income (versus lower family income, OR = 0.45), surrounded with smoking friends (versus without smoking friends, OR= 0.18), were more vulnerable to smoke. In addition, combined with the theory of planned behavior (TPB), the dimensions of learning burnout had full mediation effects between psychological distress and smoking behavior. Conclusions: Psychological distress can only indirectly affect smoking behavior via learning efficacy, cynicism and emotional exhaustion. Adjustment from different dimensions of learning burnout will help college students better prevent the tobacco use.
Development And Validation of a Risk Scoring System to Predict Pneumothorax in CT-Guided Percutaneous Transthoracic Needle Biopsy
Authors: Yang Qiuhong , Luo Lin cheng , Peng Xinyi , Wei Hailong , Yi Qun , Luo Wei 
Published: December 2021

Abstract
Objective: To develop and validate a risk scoring system using variables easily obtained for the prediction of pneumothorax in CT-guided percutaneous transthoracic needle biopsy (PTNB).Methods: The derivation cohort was comprised of 1001 patients who underwent CT-guided PTNB. Multivariate logistic regression was used to identify risk factors for pneumothorax, which were treated as the foundation to develop the risk scoring system. To validate the system, a validation cohort group of 230 patients was enrolled.Results: Age, puncture times, puncture depth, smoking index, number of specimens, bleeding from the needle path, and lobular lesion were identified as risk factors in the derivation cohort. A risk scoring system (Hosmer-Lemeshow goodness-of-fit test p =0.33) was developed. The area under the receiver operating characteristic curve (AUROC) was 0.601 by using the risk score system. This risk score system demonstrated a better diagnostic effect with increasing age. In the group of patients older than 80 years, the AUROC was 0.76, showing good predictive power. This risk scoring system was confirmed in the validation cohort with an AUROC of 0.736.Conclusion: This scoring system has a good predictive effect in both derivation and validation cohort.
Octopamine Signaling Via Oamb is Essential for A Well-Orchestrated Climbing Performance of Adult Drosophila Melanogaster
Authors: El-Kholy Samar Ezzat , Afifi Basma , El-Husseiny Iman , Seif Amal 
Published: December 2021

Abstract
The biogenic amine octopamine (OA) orchestrates many behavioural processes in insects. OA mediates its function by binding to OA receptors belonging to the G protein-coupled receptors superfamily. Despite the potential relevance of OA for controlling locomotion, our knowledge about the role of each octopaminergic receptor still limited. In this study, RNA interference (RNAi) was used to knockdown each OA receptor type in almost all Drosophila melanogaster tissues using a tubP-GAL4 driver to investigate the loss of which receptor affects the climbing ability of adult flies. The results demonstrated that oamb-deficient flies had impaired climbing ability more than those deficient in other receptors receptive for OA. Targeted RNAi-mediated kockdown of oamb in the nervous system or muscular system decreased the climbing ability, indicating that within Drosophila legs, OA through oamb orchestrated the nervous system control and muscular tissue responses. Oamb-deficient adult males showed morphometric changes in the length and width of leg parts. Transmission electron microscopy revealed that the leg muscles oamb-deficient flies have severe ultrastructural changes compared to those of control flies. The severe impairment in the climbing performance of oamb-deficient flies correlates well with the completely distorted leg muscle ultrastructure in these flies. Taken together, we could conclude that OA via oamb plays an important role in the locomotor activity of Drosophila.
Proportions of Beneficial Factors in MLR, NLR, PLR and D-dimer in Preoperative Peripheral Blood of Patients With Early Stage Lung Cancer as Predictors of Patient Survival After Surgery
Authors: wang jun , li huawei , xu ran , lu tong , zhao jiaying , zhang Pengfei , qu lidong , zhang Shengqiang , guo Jida , zhang Linyou 
Published: December 2021

Abstract
ObjectiveThe purpose of this paper is to predict the following items. preoperative baseline monocyte-to-lymphocyte ratio (MLR)、neutrophil-to-lymphocyte ratio (NLR) Platura-to-lymphocyte ratio (PLR) and dimeric fibrin fragment D (D-dimer) associated with clinical outcome in patients with Early Lung Cancer (LC).MethodsWe performed a retrospective analysis of 376 patients with LC. Progression-free survival (PFS) and overall survival (OS) were assessed by Kaplan-Meier, and univariate and multivariate Cox regression analyses were performed to identify prognostic factors. Finally, multivariate Cox regression analysis was used to evaluate the influence of favorable factors on patients’ OS and PFS combined with the basic clinical characteristics of the patient ResultsAmong the variables screened by univariate Cox regression, MLR < 0.22, NLR < 1.99, PLR < 130.55 and D-Dimer < 70.5 (ng/ml) were significantly associated with both better OS and PFS. In multivariate Cox regression analysis, it was determined that MLR and D-Dimer had a better independent correlation with OS (p = 0.009, p = 0.05, respectively), while MLR was only better independently associated with PFS (P = 0.005). Furthermore, according to the number of favorable factors, patients with none of these factors had a significantly worse prognosis than patients with at least one of these factors.Conclusion﻿Baseline characteristics of low MLR, low NLR, low PLR and low D-dimer were associated with better outcomes.
Cascade position- torque control strategy based on function approximation technique (FAT) for flexible joint robots
Authors: Al-Shuka Hayder F.N. , Corves Burkhard , Abbas Ehab N. 
Published: December 2021

Abstract
This work deals with control of rigid link robotic manipulators provided with flexible joints. Due to presence of flexible joint dynamics, additional degrees of freedom and underactuation are developed that would complicate the control design. Besides, model uncertainties, unmodeled dynamics and disturbances should be considered in robot modeling and control. Therefore, this paper proposes a cascade position-torque control strategy based on function approximation technique (FAT). The key idea is to design two nested loops: 1) an outer position control loop for tracking reference trajectory, and 2) an inner joint torque control loop to track the desired joint torque resulted from the outer position loop. The torque control loop makes the robot system more adaptable and compliant for sudden disturbances. It increases the perception capability for the target robot mechanisms. Adaptive approximation control (AAC) is used as a strong tool for dealing with time-varying uncertain parameters and disturbances. A sliding mode term is easily integrated with control law structure; however, a constraint on feedback gains are established for compensating modeling (approximation) error. The proposed control architecture can be easily used for high degrees of freedom robotic system due to the decentralized behavior of the AAC. A two-link manipulator is used for simulation experiments.The simulated robot is commanded to move from rest to desired step references considering three cases depending on the selected value of the sliding mode time constant. It is shown that selection of a large time constant parameter related to the position loop leads to slow response. Besides, one of the inherent issues associated with the inner torque control is the presence of derivative of desired joint torque that makes the input control abruptly jumping at the beginning of the dynamic response. To end this, an approximation for derivative term of the desired joint torque is established using a low-pass filter with a time constant selected carefully such that a feasible dynamic response is ensured.The results show the effectiveness of the proposed controller.
Complete mineralization of acetic acid using catalytic ozonation with a high energy efficiency enhanced by MnO2/γ-Al2O3
Authors: Meng Ruiyun , Yao Shuiliang , Gao Erhao , Wu Zuliang , Li Jing , Du Erdeng 
Published: December 2021

Abstract
The complete mineralization of acetic acid in a biodegradation process is difficult due to the α-position methyl on the carboxyl group of acetic acid. This study explores the complete oxidation of acetic acid by catalytic ozonation. Metal oxides of MnO2, Co3O4, Fe3O4, and CeO2 loaded on γ-Al2O3 power were used as the catalysts. The experimental results showed that MnO2/γ-Al2O3 catalyst had the best mineralization performance for acetic acid. Typically, the mineralization of acetic acid is as high as 88.4% after 300 min ozonation of 100 mL of 1.0 g L‒1 acetic acid catalysed by 3.0 g 1.0wt.% MnO2/γ-Al2O3 catalyst powder with an energy efficiency of 15 g kWh‒1. However, without a catalyst, the mineralization of acetic acid is only 33.2% with an energy efficiency of 5.1 g kWh−1. The effects of MnO2 loading, catalyst dosage, acetic acid concentration, O3 concentration, ozonation temperature, and initial pH value of the acetic acid solution were systematically investigated. Radical quenchers and in-situ DRIFTS analyses indicated that •OH radical and reactive oxygen species on catalyst surface played an important role in the ozonation of acetic acid to CO2 and H2O. The mechanism of acetic acid oxidation on MnO2/γ-Al2O3 is proposed.
P. Edulis Extract Protects Against Amyloid-β Toxicity in Alzheimer’s Disease Models Through Maintenance of Mitochondrial Homeostasis via the FOXO3/DAF-16 Pathway
Authors: Cao Shu-qin , Aman Yahyah , Fang Evandro Fei , Tencomnao Tewin 
Published: December 2021

Abstract
Alzheimer’s disease (AD) is a common and devastating disease characterized by pathological aggregations of beta-amyloid (Aβ) plaques extracellularly, and Tau tangles intracellularly. While our understandings of the aetiologies of AD have greatly expanded over the decades, there is no drug available to stop disease progression. Here, we demonstrate the potential of P. edulis pericarp extract in protecting against Aβ-mediated neurotoxicity in mammalian cells and Caenorhabditis elegans models of AD. We show P. edulis pericarp protects against memory deficit, neuronal loss, and promotes longevity in the Aβ model of AD via stimulation of mitophagy, a selective cellular clearance of damaged and dysfunctional mitochondria. P. edulis pericarp also restores memory and increases neuronal resilience in a C. elegans Tau model of AD. While defective mitophagy-induced accumulation of damaged mitochondria contributes to AD progression, P. edulis pericarp improves mitochondrial homeostasis through NIX/DCT1-dependent mitophagy and SOD3-dependent mitochondrial resilience, both via increased nuclear translocation of the upstream transcriptional regulator FOXO3/DAF-16. Further studies to identify active molecules in P. edulis pericarp that could maintain neuronal mitochondrial homeostasis may enable the development of potential drug candidates for AD.
Simplified Chinese Version of the International Prostate Symptom Score and the Benign Prostatic Hyperplasia Impact Index: Cross-Cultural Adaptation, Reliability, and Validity for Patients with Benign Prostatic Hyperplasia
Authors: Dun Rong-liang , Mao Jian-min , Yu Chao , Zhang Qiang , Hu Xiao-hua , Zhu Wen-jing , Qi Guang-chong , Peng Yu 
Published: December 2021

Abstract
Purpose: The aim of this study was to translate and cross-cultural adapt the international prostate symptom score (IPSS) and benign prostatic hyperplasia impact index (BII) into simplified Chinese for mainland Chinese patients with benign prostatic hyperplasia (BPH).Methods: The original English IPSS and BII were translated into simplified Chinese versions, based on cross-cultural adaptation guidelines. Internal consistency was evaluated with Cronbach’ α, then test-retest reliability with intraclass correlation coefficients (ICCs) in stable patients. The validity of these two adaptations was tested by the correlation between the IPSS, and BII with visual prostate symptom score (VPSS), and 36 items Short Form Health Survey (SF-36). The floor and ceiling effects were calculated by the proportion of participants who obtained the highest and lowest possible score.Results: A total of 105 native Chinese-speaking patients with BPH were enrolled. Cronbach’ α were over 0.75 for the simplified Chinese IPSS (IPSS 0.815; IPSS-symptom 0.782), and 0.709 for the simplified Chinese BII, indicating acceptable internal consistency. The ICCs for the test-retest reliability were over 0.75 (IPSS, r = 0.836; IPSS-symptom, r = 0.801; IPSS-quality of life, r = 0.794; BII, r = 0.758), indicating excellent test-retest reliability. There were very good positive correlations between IPSS and BII (r = 0.605), as well as VPSS (r = 0.634), and very good or good negative correlations between IPSS-Qol and SF-36 physical functioning (r = -0.621), and vitality (r = -0.659), and between BII and SF-36 physical functioning (r = -0.421). No floor or ceiling effect was detected in the simplified Chinese IPSS and BII.Conclusions: This study indicates that the simplified Chinese IPSS and BII are reliable and valid measurement of the symptom and quality of life among Chinese patients with BPH, which is likely to be widely used in this population.