bluuebunny/crossref_metadata_2025_split huggingface dataset:
Impacts of a delayed and slow-paced vaccination on cases and deaths during the COVID-19 pandemic: a modelling study
Authors: Libotte Gustavo , Anjos Lucas , de Almeida Regina Célia Cerqueira , Malta Sandra Mara , Medronho Roberto 
Published: November 2021

Abstract
Background: In Brazil, vaccination has always been cutting across party political and ideological lines, which have delayed its start and brought the whole process into disrepute. Such divergences put the immunisation of the population in the background and create additional hurdles beyond the pandemic, mistrust and scepticism over vaccines.Methods: We conduct a mathematical modelling study to analyse the impacts of late vaccination and with slowly increasing coverage, as well as how harmful it would be if part of the population refused to get vaccinated or missed the second dose. We analyse data from confirmed cases, deaths caused by COVID-19, and vaccination in the state of Rio de Janeiro in the period between March 10, 2020, and October 27, 2021. The classical SIR model is extended to consider the effect of vaccination (efficacy, interval between doses, and vaccination rate) and data sets are regularised using Gaussian Process Regression. The model parameter distributions are estimated using Bayesian inference, aiming to obtain credible intervals in the simulations.Findings: We estimate that if the start of vaccination had been 30 days earlier, combined with efforts to drive vaccination rates up, 31,657 (25,801–35,117) deaths could have been averted. Our results also indicate that the slow pace of vaccination and the low demand for the second dose could cause a resurgence of cases as early as 2022.Interpretation: The government's inaction and lack of a strategic plan to fight the pandemic meant that vaccination started late, leading to thousands of deaths that could have been prevented. Even when reaching the expected vaccination coverage for the first dose, it is still challenging to increase adherence to the second dose and maintain a high vaccination rate to avoid new outbreaks.Funding: Carlos Chagas Filho Foundation for Supporting Research in the State of Rio de Janeiro (FAPERJ) and Brazilian National Council for Scientific and Technological Development (CNPq).
Spa Diversity and Genetic Characterization of t127 Methicillin-resistant Staphylococcus Aureus in a Tertiary Greek Hospital
Authors: Tsergouli Katerina , Karampatakis Theodoros , Kontopoulou Konstantina , Pappa Styliani , Kampouridou Parthena , Kallasidou Georgia , Tsioka Katerina , Zotou Sophia , Farmaki Eleftheria-Eugenia , Kotzamanidis Charalampos , Papa Anna 
Published: November 2021

Abstract
Introduction: Methicillin-resistant Staphylococcus aureus (MRSA) causes severe community and hospital acquired infections. Identification of staphylococcal cassette chromosome mec (SCCmec), multilocus-sequence typing, and sequencing of S. aureus protein A (spa) gene are used for MRSA typing. The aim was to investigate the spa types of MRSA isolates in a tertiary hospital in Greece and analyse the whole genome sequences of two t127 MRSA isolates. Methods: Totally, 39 MRSA isolates collected during July 2019 to June 2020 in “Georgios Gennimatas” General Hospital of Thessaloniki, Greece, were included in the study. Identification and antimicrobial susceptibility testing were performed using VITEK II automated system, and spa typing was performed. A minimum spanning tree was used to display the spa type frequencies and the genetic distances among them. Two t127-MRSA isolates (IM-MRSA and PD-MRSA) were selected for WGS.Results: Twenty-two different spa types were detected, with t002, t003, and t422 being the most frequent (5/39, 12.8% each), followed by t1994 (4/39, 10.3%). The isolates presented high genetic diversity and, taking into account the time between hospital admission and sampling, intrahospital spread did not occur. Even the two t127 isolates were assigned to different sequence types, ST9-XII-t127 and ST1-IVa-t127. Plasmids and genes conferring antimicrobial resistance and virulence were also identified.Conclusions: Various spa types were identified and together with the information about the time between hospital admission and sampling supports polyclonal MRSA spread in the hospital excluding a nosocomial infection. WGS provides a more detailed analysis distinguishing even the isolates belonging to the same spa type.
ASXL1 Mutations with Serum EPO Levels Predict a Poor Response to Darbepoetin Alfa in Lower-Risk MDS: W-JHS MDS01 Trial
Authors: Morita Yasuyoshi , Nannya Yasuhito , Ichikawa Motoshi , Hanamoto Hitoshi , Shibayama Hirohiko , Maeda Yoshinobu , Hata Tomoko , Miyamoto Toshihiro , Kawabata Hiroshi , Takeuchi Kazuto , Kishimoto Junji , Tanaka Hiroko , Miyano Satoru , Matsumura Itaru , Ogawa Seishi , Akashi Koichi , Kanakura Yuzuru , Mitani Kinuko 
Published: November 2021

Abstract
Darbepoetin alfa (DA) is often used in treating anemia of lower-risk (IPSS low or int-1) myelodysplastic syndromes (MDS). However, whether mutations can predict effectiveness of DA has not been examined. The present study aimed to determine gene mutations for predicting therapeutic effect of DA. Primary endpoint was correlation between the presence of highly frequent (≥10%) mutations and hematological improvement erythroid according to IWG criteria 2006 by DA (240 μg/week) until week 16. Included were 79 patients (age 29-90, median 77.0 years; 52 [65.8%] male). Frequently (≥10%) mutated genes were SF3B1 (24 cases, 30.4%), TET2 (20, 25.3%), SRSF2 (10, 12.7%), ASXL1 (9, 11.4%), and DNMT3A (8, 10.1%). Overall response rate to DA was 70.9%. Multivariable analysis including baseline erythropoietin levels and red blood cell transfusion volumes as variables revealed that erythropoietin levels and mutations of ASXL1 gene were significantly associated with worse response (odds ratio 0.146, 95% confidence interval 0.042-0.503; p=0.0023, odds ratio 0.175, 95% confidence interval 0.033-0.928; p=0.0406, respectively). This study indicated that anemic patients who show higher erythropoietin levels and harbor ASXL1 gene mutations may have poor response to DA. The alternative strategies are needed for the treatment of anemia in this population. Trial registration number and date of registration: UMIN000022185 & 09/05/2016.
Theoretical and Experimental Investigation of the Electronic and Optical Properties of Pure and Interstitial Nitrogen -Doped (TiO2)n Cluster
Authors: Kakil Shaida , Abdullah Hewa Y , Abdullah Tahseen G. 
Published: November 2021

Abstract
The structural and electronic properties of pure and nitrogen-doped TiO2 nanoclusters are investigated using density functional theory (DFT) with vibrational modes. We performed numerical simulation using two methods based on theories at the Quantum Espresso/PBE and Gaussian/B3LYP/631G (d) levels. The properties of a single nitrogen-doped (TiO2)n nanocluster are also computed in this study. In both cases, interstitial and substitutional Nitrogen doping at all accessible sites was examined. For the experiment, Supersonic Cluster Beam Deposition (SCBD) was used to create pure and nitrogen-doped TiO2 films of nanocluster assemblies. Atomic force microscopy (AFM), X-ray photoelectron spectroscopy (XPS), UV-Vis spectroscopy, and Raman techniques were used to characterize these samples. The binding energies (Np, O2s, Ti 2p1/2, and Ti 2p3/2) of N-doped TiO2 were estimated using XPS spectral results. The UV-Vis measurement confirmed the previously stated reasoning about the quantum size effect on the band gap of the pure and nitrogen doped TiO2 nanocluster. The theoretical vibrational modes frequencies are calculated using the B3LYP/6-31G (d) functional via the Gaussian16 code's implementation algorithm. The good agreement between simulation and experimental results implies that a significant advantage of interstitial over substitutional positions. N-O vibration modes appeared in interstitial doped TiO2, and each vibration was dependent on a different cluster structure.
Assessment of Genetic Diversity and Relationship of the Two Sanga Type Cattle of Botswana Based on Microsatellite Markers
Authors: Bakae Tirelo , Monau Phetogo Ineeleng , Nsoso James , Kgwatalala Patrick Monametsi 
Published: November 2021

Abstract
The study was performed to evaluate genetic variation on two Sanga type cattle found in Botswana; Tswana and Tuli using twelve microsatellite markers. All amplified loci were polymorphic with 75 and 77 alleles genotyped in the Tswana and Tuli populations, respectively. The total number of alleles per locus ranged from 2 (BM1818) to 10 (TGLA227) with total mean of 6.25 for Tswana and 6.43 for Tuli population. Almost all the markers showed high polymorphic information content (PIC) apart from BM1818 (0.375) and INTRA23 (0.393) which were moderately informative in Tswana population. Most of the markers were in Hardy-Weinberg equilibrium except for CSSRM60 and CSSM66 loci in Tswana population and ETH10, ETH225 and CSSM66 loci in Tuli population. A total of 103 unique alleles were genotyped across the two breeds with 49-shared, and 26 and 28 were unique to Tswana and Tuli populations, respectively. The expected heterozygosity (He) values were higher than the observed heterozygosity (Ho) in both populations; Tswana (He=0.7895±0.033 vs Ho=0.631±0.091) and Tuli (He=0.8123±0.033 vs Ho=0.556±0.021). The inbreeding coefficient was 0.200±0.002 and 0.332±0.001 in Tswana and Tuli populations, respectively. Analysis of molecular variance revealed 6.8% of the total genetic variation corresponding to differences between the two breeds and 93.2% within populations. The genetic identity between the two breeds was 56% and there were similar levels of multilocus heterozygosity and allelic diversity in the two breeds. The use of Tswana and Tuli breeds in a crossbreeding program is likely to result in minimal heterosis and therefore not recommended.
Impact of the absence of dental support on cancer patients during the COVID-19 pandemic
Authors: Reia Verônica Caroline Brito , Robles-Mengoa Maria Gabriela , Orcina Bernardo da Fonseca , Quenta-Huayhua Milenka Gabriela , Carneiro Mailon Cury , Manzano Brena Rodrigues , Prestes Anderson , Rubira Cássia Maria Fischer , Santos Paulo Sérgio da Silva 
Published: November 2021

Abstract
Purpose

With the suspension of routine services due to the coronavirus disease-2019 (COVID-19) pandemic, a significant number of the population has been presenting acute oral alterations without proper treatment. Through telecontact, this study aimed to identify individuals in treatment and had been treated for cancer who had their clinical dental care interrupted by the COVID-19 pandemic.
Methods

Individuals with oncologic diseases were selected from a telephone list of a clinical research center specializing in the care of cancer patients. We included those who answered an online questionnaire about their general health status and oral problems when they were unable to access dental care during the pandemic. Statistical analysis was performed using Fisher and Chi-square tests. The significance level was set at 5% (p<0.05).
Results

Of the 280 patients recruited, 104 answered the questionnaire. There were 75 (72.1%) were women, of which 22 (36.7%) were under antineoplastic treatment, and 30 (68.2%) had already been treated; 29 (27.9%) were men, of which 15 (25.0%) were under antineoplastic treatment and 14 (31.8%) had already been treated. Of the universal sample, 64 (61.5%) had no complaints regarding their general health, and 74 (71.1%) had no difficulties in their daily activities due to teeth/mouth problems. However, the rates of oral problems were not statistically significant (p=NS).
Conclusion

In dentistry, telephone support and digital tools are useful and necessary instruments for the follow-up of cancer patients during the pandemic.
Hydroxycinnamic Acids and Their Related Synthetic Analogs: An Update
of Pharmacological Activities
Authors: Selka Ayyoub , Moutombi Fanta J. Ndongou , Jean-François Jacques , Touaibia Mohamed 
Published: November 2021

:
The hydroxycinnamic acid scaffold is extremely versatile with various biological activities.
This review will highlight the progress of the biological activities of hydroxycinnamic acids and their
related synthetic analogs, including recently reported anti-cancer, anti-inflammatory, and antioxidant
activities.
EDL933 Strains of Escherichia coli O157 can Demonstrate Genetic Diversity and Differential Adherence to Bovine Recto-Anal Junction Squamous Epithelial Cells
Authors: S. Hoefler Raegan , Kudva Indira T. 
Published: November 2021

Background:Differences betweenEscherichia coliO157 (O157) strains are well-established with some of these strains being associated with major outbreaks in the US. EDL933 is one such O157 strain that caused a multistate outbreak in 1982 and has since been used as a prototype in various O157-related experiments.Objective:As O157 can readily acquire genetic mutations, we sought to determine if the genetic and phenotypic profiles of EDL933 strains from different sources would be consistent.Methods:We evaluated wild-type O157 strains stocked as EDL933 from three different laboratories, in the strain typing Polymorphic Amplified Typing Sequence (PATS) and the bovine rectal-anal junction squamous epithelial (RSE) cell- and HEp-2 cell- adherence assays. In addition, we also verified if Shiga toxins (Stx), the Locus of Enterocyte Effacement (LEE) or curli fimbriae contributed to the adherence phenotypes observed using mutant and wild-type EDL933 isolates.Results:Our results showed differences in PATS profiles and RSE cell-adherence phenotype, with no influence from the Stx or LEE genes, between EDL933 from different sources. Interestingly, the EDL933 strain that demonstrated the most contrasting diffuse adherence phenotype on RSE cells, EDL933-T, had decreased curli production that may have contributed to this phenotype.Conclusion:Our observations suggest that a comprehensive characterization of bacterial isolates, even if assigned to the same strain type prior to use in experiments, is warranted to ensure consistency and reproducibility of results.
The Impact of Quarantine on Emotions during the COVID-19 Pandemic
Authors: Chomentauskas Gintaras , Dereškevičiūtė Edita , Kalanavičiūtė Gustė , Ališauskienė Rasa , Paulauskaitė Kristina 
Published: November 2021

Introduction:
The present study explores how the general population of Lithuania felt during the national quarantine of COVID-19 in the period from March 30th to June 8th, 2020. Representative subjects were interviewed five times using adapted single-item questions to evaluate their emotions, stress, and perceived pain levels.

Methods:
It was hypothesized that gender and age-related differences would be found in emotional responses to the quarantine.

Results and Discussion:
During the five polls taken, more women were found to report feeling stressed, anxious, sad, and in more physical pain than men. Evaluations of anger, enjoyment, and calmness provided no statistically significant gender differences. Emotions were found to differ significantly between three age groups (18-29, 30-49, 50-74).

Conclusion:
Contrary to expectations, the biggest negative impact of quarantine was found in the youngest group aged 18-29 years: it showed the highest prevalence of stress, anxiety, and sadness of all age groups. Possible explanations for different gender and age-related emotional reactions are discussed.
Factors Hampering the Participation of Nursing Staff in the Continuing Education Activities of Hospitals Centers in the Casablanca-Settat Region
Authors: Lhbibani Amina , Lamiri Abderrahmane , Lotfi Said , Tridane Malika , Belaaouad Said 
Published: November 2021

Aim:This study aimed to identify the factors hampering the participation of nurses in the activities of CE sessions at the level of hospitals in the region of Casablanca.Background:Continuing education (CE) for nursing staff represents a strategic asset for hospitals, identifying the constraints of nursing staff participation in continuing training could help improve the quality of care for patients and the population in general.Objective:This study aimed to identify the factors hampering the participation of nurses in the activities of CE sessions at the level of hospitals in the region of Casablanca.Methods:The study used a two-phase mixed method design. First of all, a questionnaire was administered to 930 nurses belonging to 9 hospital centers in the Casablanca-Settat region in order to explore and estimate the frequencies of the factors hindering the participation of nurses in the activities of the FC sessions at the level of hospitals, and a semi-structured interview with 9 persons in charge of continuing education from these different hospitals to complete and explain the data collected by the questionnaire.Results:The data analysis confirmed that the work overload is the first individual difficulty hindering the participation of nurses in CE sessions,i.e., 85.4%. The most mentioned organizational difficulties are schedule recommended in the FC not encouraging and not suitable,i.e., 23%, non-targeted content (does not meet the needs of the nursing staff),i.e., 66.7%. Finally, the absence of support measures in terms of monitoring and evaluation to maintain the knowledge and skills acquired during FC sessions in real situations at the workstation occupied is the first institutional difficulty mentioned by the interviewed (88%).Conclusion:Those responsible for training should take into account the factors nurse’s face in participating in continuing education sessions when designing, developing, and implementing continuing education sessions.
Algorithm to construct integro splines
Authors: Mijiddorj Renchin-Ochir , Zhanlav Tugal 
Published: November 2021

We study some properties of integro splines. Using these properties, we design an algorithm to construct splines \(S_{m+1}(x)\) of neighbouring degrees to the given spline \(S_{m}(x)\) with degree \(m\). A local integro-sextic spline is constructed with the proposed algorithm. The local integro splines work efficiently, that is, they have low computational complexity, and they are effective for use in real time. The construction of nonlocal integro splines usually leads to solving a system of linear equations with band matrices, which yields high computational costs.

doi:10.1017/S1446181121000316
Spelling Bee Competition Trophy Rector of Bina Darma 2018
Authors: Kurniawan Ferry , Ariana Sunda , Rosmaidar Rosmaidar 
Published: November 2021

Global competition has demanded every individual to have strong competitiveness on a national and international scale. Proficiency in language is also a demand in global competition. Language is also a medium that can influence someone and is a media used to express opinions and analyze problems. As a lecturer who takes shelter in the English study program, there is a desire to conduct guidance in the language field for lecturers and employees of Bina Darma University as one of the community service activities. Therefore Bujang Girls Campus Bina Darma University was chosen to conduct Spelling Bee Competition activities to provide motivation to learn English for lecturers and employees of Bina Darma University .. The mission of the Spelling Bee Comepetion activity is to provide additional knowledge of English which is expected to be able to increase English proficiency for Bina Darma University lecturers and employee.

Keywords: Spelling, Bee, Competition
Analisis Vo2Max Melalui Velocity Critical Run Tim Futsal Putra SMA Plus PGRI Cibinong
Authors: Fitranto Nur , Apriyanto Tirto , Octyawan Mohamad Rizal 
Published: November 2021

Tujuan penelitian ini adalah untuk mengetahui Velocity Critical Run Tim futsal putra Sekolah menengah atas Plus PGRI Cibinong, menganalisis seberapa jauh jarak para pemain melakukan aktifitas fisik dengan bejalan ,berlari dan sprint serta menganalisis seberapa lama durasi yang ditempuh para pemain dengan berjalan berlari, dan sprint pada Kejuaraan Allievo Futsal Championship. Penelitian ini dilaksanakan di Gor Laga tangkas Kab. Bogor tanggal 21 – 26 februari 2020 dan metode yang dipergunakan dalam penilitian ini adalah metode analisis deskriptif. Teknik pengambilan samplenya yaitu total Sampling atau teknik pengambilan sampel total dimana jumlah sampel sama dengan populasi. Keseluruhan pemain futsal putra Sekolah menengah atas Plus PGRI Cibinong yang bertanding di Allievo Futsal Championship sebanyak 12. Hasil pertama pada penelitian adalah Jarak Velocity Critical Run Tim Futsal Putra Sekolah menengah atas Plus PGRI Cibinong pada Kejuaraan Allievo Futsal Championship terjadi sebanyak 32006 meter. Dari hasil tersebut rata-rata pemain dalam melakukan aktifitas fisik adalah hanya 1,9 m/s maka dapat disimpulkan pada pertandingan tersebut para pemain Futsal Putra SMA Plus PGRI Cibinong dalam tiga pertandingan melakukan aktfitias dengan intesitas rendah. Dengan demikian Vcr para pemain futsal Tim Futsal SMA Plus PGRI Cibinong harus ditingkatkan 2.2 m/s -2.4 m/s dari indikator 70-75% dari VO2max.
Kata kunci — Analisis, Vo2Max, Velocity Critical Run (VCR), Futsal
First report of an important sheat nematode, Hemicycliophora poranga, associated with sugar beet (Beta vulgaris L.) in Vietnam
Authors: Nguyen T. D. , Trinh Q. P. 
Published: November 2021

Summary
Several species of the sheat nematodes, Hemicycliophora spp., have been known to cause significant damage to agricultural crops, including Hemicycliophora arenaria, H. conida, H. parvana, H. poranga, H. similis, and H. typica. Remarkably, our study reported on the presence of H. poranga for the first time in Vietnam. This species was found on 83.33% of the total samples with an average density of 270 individuals/100ml of soil (positive samples). In this study, the Vietnamese population of H. poranga was characterized based on both morphology and molecular characterization of D2-D3 expansion segment of 28S rRNA sequence. Besides, a molecular phylogenetic tree of the genus Hemicycliophora was also provided.