bluuebunny/crossref_metadata_2025_split huggingface dataset:
Field multi-omics analysis reveals a close association between bacterial communities and mineral properties in the soybean rhizosphere
Authors: Yamazaki Shinichi , Mardani-korrani Hossein , Kaida Rumi , Ochiai Kumiko , Kobayashi Masaru , Nagano Atsushi J. , Fujii Yoshiharu , Sugiyama Akifumi , Aoki Yuichi 
Published: April 2021

AbstractThe plant root-associated environments such as the rhizosphere, rhizoplane, and endosphere are different from the outer soil region (bulk soil). They establish characteristic conditions including microbiota, metabolites, and minerals, and they can directly affect plant growth and development. However, comprehensive insights into those characteristic environments, especially the rhizosphere, and molecular mechanisms of their formation are not well understood. In the present study, we investigated the spatiotemporal dynamics of the root-associated environment in actual field conditions by multi-omics analyses (mineral, microbiome, and transcriptome) of soybean plants. Mineral and microbiome analyses demonstrated a characteristic rhizosphere environment in which most of the minerals were highly accumulated and bacterial communities were distinct from those in the bulk soil. Mantel’s test and co-abundance network analysis revealed that characteristic community structures and dominant bacterial taxa in the rhizosphere significantly interact with mineral contents in the rhizosphere, but not in the bulk soil. Our field multi-omics analysis suggests a rhizosphere-specific close association between the microbiota and mineral environment.
Parameter study of the high temperature MOCVD numerical model for AlN growth using orthogonal test design
Authors: An Jiadai , Dai Xianying , Feng Lansheng , Zheng Jieming 
Published: April 2021

AbstractWe investigated the process parameters of the high temperature MOCVD (HT-MOCVD) numerical model for the AlN growth based on CFD simulation using orthogonal test design. It is believed that high temperature growth condition is favorable for improving efficiency and crystallization quality for AlN film, while the flow field in the HT-MOCVD reactor is closely related to the process parameters, which will affect the uniformity of the film. An independently developed conceptual HT-MOCVD reactor was established for the AlN growth to carry out the CFD simulation. To evaluate the role of the parameters systematically and efficiently on the growth uniformity, the process parameters based on CFD simulation were analyzed using orthogonal test design. The advantages of the range, matrix and variance methods were considered and the results were analyzed comprehensively and the optimal process parameters were obtained as follows, susceptor rotational speed 400 rpm, operating pressure 40 Torr, gas flow rate 50 slm, substrate temperature 1550 K.
Deep genetic structure at a small spatial scale in the endangered land snail Xerocrassa montserratensis
Authors: Català Cristina , Bros Vicenç , Castelltort Xavier , Santos Xavier , Pascual Marta 
Published: April 2021

AbstractSpecies with small geographic ranges do not tend to have a high genetic structure, but some land snail species seem to be an exception. Xerocrassa montserratensis, an endangered land snail endemic to Catalonia (northeastern Iberian Peninsula), is an excellent model to study the processes affecting the phylogeography of specialized species of conservation concern. This species is restricted to xerophilous stony slopes and occurs within a small and fragmented area of ca. 500 km2. We sequenced the COI barcode region of 152 individuals from eight sites covering the entire range of the species. We found four genetic groups mostly coincident with their geographic distribution: a central ancestral group containing shared haplotypes among five localities and three groups restricted to a single locality each. Two of these derived groups were geographically and genetically isolated, while the third and most differentiated group was not geographically isolated. Geomorphologic and paleoclimatic processes during the Pleistocene can explain the divergence found between populations of this low dispersal species with historical fragmentation and secondary contacts. Nonetheless, recent passive large dispersal through streams was also detected in the central group. Overall, our study uncovered four evolutionary units, partially matching morphologically described subspecies, which should be considered in future conservation actions.
An advancement in the synthesis of unique soft magnetic CoCuFeNiZn high entropy alloy thin films
Authors: Pavithra Chokkakula L. P. , Janardhana Reddy Kunda Siri Kiran , Reddy Kolan Madhav , Murapaka Chandrasekhar , Joardar Joydip , Sarada Bulusu V. , Tamboli Rameez R. , Hu Yixuan , Zhang Yumeng , Wang Xiaodong , Dey Suhash Ranjan 
Published: April 2021

AbstractDiscovery of advanced soft-magnetic high entropy alloy (HEA) thin films are highly pursued to obtain unidentified functional materials. The figure of merit in current nanocrystalline HEA thin films relies in integration of a simple single-step electrochemical approach with a complex HEA system containing multiple elements with dissimilar crystal structures and large variation of melting points. A new family of Cobalt–Copper–Iron–Nickel–Zinc (Co–Cu–Fe–Ni–Zn) HEA thin films are prepared through pulse electrodeposition in aqueous medium, hosts nanocrystalline features in the range of ~ 5–20 nm having FCC and BCC dual phases. The fabricated Co–Cu–Fe–Ni–Zn HEA thin films exhibited high saturation magnetization value of ~ 82 emu/g, relatively low coercivity value of 19.5 Oe and remanent magnetization of 1.17%. Irrespective of the alloying of diamagnetic Zn and Cu with ferromagnetic Fe, Co, Ni elements, the HEA thin film has resulted in relatively high saturation magnetization which can provide useful insights for its potential unexplored applications.
The BET inhibitor attenuates the inflammatory response and cell migration in human microglial HMC3 cell line
Authors: Baek Mina , Yoo Eunyoung , Choi Hae In , An Ga Yeong , Chai Jin Choul , Lee Young Seek , Jung Kyoung Hwa , Chai Young Gyu 
Published: April 2021

AbstractMicroglia, resident macrophages of the brain that act as primary immune cells, play essential roles in innate immunity and neuroinflammatory pathologies. Microglial cells are rapidly activated in response to infection and inflammation/injury, associated with the expression of proinflammatory genes and secretion of cytokines. The bromodomain and extra-terminal (BET) inhibitor JQ1 has been shown to be an epigenetic agent that reduces inflammation. In this study, we investigated the mechanisms underlying the anti-inflammatory and anti-migratory functions of JQ1 and the genes targeted by JQ1 in lipopolysaccharide (LPS)-activated human microglial clone 3 (HMC3) cells using RNA-sequencing (RNA-seq). We analyzed the pattern of inflammation-related genes (chemokines, cytokines, and interferon-stimulated genes) and migration-related genes with JQ1 treatment from differentially expressed genes analysis in HMC3 cells. We found that LPS-induced IRF1 directly regulated inflammation- and migration-related genes and that JQ1 significantly reduced IRF1 and its target genes. Additionally, IRF1 attenuation significantly downregulated target genes and inhibited microglial migration. Our data suggest that the BET inhibitor JQ1 can modulate the inflammatory response and migration through the regulation of LPS-induced IRF1 in human microglia.
A computer study of the risk of cholesterol gallstone associated with obesity and normal weight
Authors: Kubica Krystian , Balbus Joanna 
Published: April 2021

AbstractObese people differ from the people of normal weight in gall bladder motility and have a higher risk of cholesterol stone formation. In this study, using a mathematical model of cholesterol homeostasis, which also considers the enterohepatic circulation of bile as well as cholesterol, we investigated the risk of cholesterol stone formation in obese and normal-weight groups who had normal blood cholesterol levels. We associated the risk of stone formation with the amount of cholesterol released into bile and the amount of de novo-synthesized cholic acid. For both groups, we determined the conditions of low and high risk. In addition, we analyzed the potential effects of changes in gall bladder motility with increased weight. The results showed that the obese group exhibited increased kinetics of enterohepatic circulation, leading to a significant increase in blood cholesterol levels, which can be reduced by increasing the amount of cholesterol in bile. Based on this finding, we suggest that for obese people, it is beneficial to reduce the amount and change the composition of circulating bile through the inhibition of cholic acid synthesis along with cholesterol synthesis. Furthermore, obese people should maintain a triglyceride-lowering diet and consume small meals containing fat, preferably in combination with agents that can reduce bile output from the gall bladder.
Social buffering in horses is influenced by context but not by the familiarity and habituation of a companion
Authors: Ricci-Bonot Claire , Romero Teresa , Nicol Christine , Mills Daniel 
Published: April 2021

AbstractSocial buffering occurs when the presence of one animal attenuates another’s stress response during a stressful event and/or helps the subject to recover more quickly after a stressful event. Inconsistent previous results might reflect previously unrecognised contextual influences, such as the nature of the stimulus presented or social factors. We addressed these issues in a two-part study of horses paired with familiar (16 subjects) or unfamiliar (16 subjects) companions. Each subject performed 4 tests in a counterbalanced order: novel object test (static ball)—alone or with companion; and umbrella opening test—alone or with companion. Social buffering was significantly influenced by the nature of the stimulus presented, but not by companion’s habituation status or familiarity. Importantly, the stimulus used produced differential effects on behavioural and physiological measures of buffering. A companion significantly reduced behavioural response (reactivity) in the novel object test but not in the umbrella test. However, heart rate recovered more quickly for subjects with a companion in the umbrella test but not in the novel object test. We propose that circumstances which permit greater contextual processing may facilitate demonstration of behavioural effects of social buffering, whereas buffering in response to startling events may be manifest only during post-event physiological recovery.
New species and a new record of Phylloporia from Benin
Authors: Olou Boris Armel , Yorou Nourou Soulemane , Langer Ewald 
Published: April 2021

AbstractSpecies of the wood-decay genus Phylloporia (Hymenochaetaceae, Hymenochaetales, Basidiomycota) are widely distributed in the tropics. Phylloporia species are, however, morphologically and ecologically diverse, which makes morphology-based species identification challenging. In this study, we re-examined species of Phylloporia reported from Benin (West Africa). Using an integrative approach combining morphology, ecology, and phylogenetic analyses, we describe Phylloporia beninensis sp. nov. and report Phylloporia littoralis for the first time outside of its type locality. Phylloporia beninensis sp. nov. is characterized by its annual and imbricate basidiomata, duplex context with a black zone separating the upper context from the lower one, dimitic hyphal system, presence of cystidioles, basidia of 9–12 × 4–5 μm, and subglobose to ellipsoid basidiospores measuring 3–4.6 × 2.1–3.6 μm. Detailed descriptions with illustrations for the new species are provided. With the addition of the new species, 15 Phylloporia species are now known to occur in tropical Africa. Our discovery of a new Phylloporia species in Benin should stimulate further mycological investigations in tropical African ecosystems to discover other new polypore species. To facilitate further taxonomy studies on tropical African Phylloporia taxa, a key to the known tropical African species is provided.
A strain rate dependent thermo-elasto-plastic constitutive model for crystalline metallic materials
Authors: Chen Cen , Wang TzuChiang 
Published: April 2021

AbstractThe strain rate and temperature effects on the deformation behavior of crystalline metal materials have always been a research hotspot. In this paper, a strain rate dependent thermo-elasto-plastic constitutive model was established to investigate the deformation behavior of crystalline metal materials. Firstly, the deformation gradient was re-decomposed into three parts: thermal part, elastic part and plastic part. Then, the thermal strain was introduced into the total strain and the thermo-elastic constitutive equation was established. For the plastic behavior, a new relation between stress and plastic strain was proposed to describe the strain rate and temperature effects on the flow stress and work-hardening. The stress–strain curves were calculated over wide ranges of strain rates (10–6–6000 s−1) and temperatures (233–730 K) for three kinds of crystalline metal materials with different crystal structure: oxygen free high conductivity copper for face centered cubic metals, Tantalum for body centered cubic metals and Ti–6Al–4V alloy for two phase crystal metals. The comparisons between the calculation and experimental results reveal that the present model describes the deformation behavior of crystalline metal materials well. Also, it is concise and efficient for the practical application.
Ultrasound use in metastatic breast cancer to measure body composition changes following an exercise intervention
Authors: Escriche-Escuder Adrian , Trinidad-Fernández Manuel , Pajares Bella , Iglesias-Campos Marcos , Alba Emilio , Cuesta-Vargas Antonio I. , Roldán-Jiménez Cristina 
Published: April 2021

AbstractChanges in body composition and muscle dysfunction are common in metastatic breast cancer (MBC). Ultrasound imaging (US) offers reliable information about muscle and fat tissue architecture (thickness) and quality (echo-intensity). This study aimed to analyze the responsiveness of thickness and echo-intensity and its possible relationship with functional and patient reported-outcomes (PRO) in MBC patients after an exercise intervention. A prospective study was conducted in 2019. A 12-week exercise program was performed, including aerobic exercise and strength training. Measurements were made at baseline and after intervention. Thickness and echo-intensity were obtained from the quadriceps and biceps brachii and brachialis (BB). Mean differences were calculated using the T-Student parametric test for dependent samples of the differences in the means before and after the intervention (p = 0.05; 95% CI). Data from 13 MBC patients showed that some US muscle variables had significant differences after intervention. Best correlations were found between the quality of life questionnaire (QLQ-BR23) PRO and variables from BB muscle thickness in contraction (r = 0.61, p < 0.01), and Non-contraction (r = 0.55, p < 0.01). BB Muscle Non-contraction Thickness also explained 70% of QLQ-BR23 variance. In conclusion, muscle architecture biomarkers showed great responsiveness and are correlated with PRO after an exercise intervention in MBC patients.
The influence of axial myopia on optic disc characteristics of glaucoma eyes
Authors: Rezapour Jasmin , Bowd Christopher , Dohleman Jade , Belghith Akram , Proudfoot James A. , Christopher Mark , Hyman Leslie , Jonas Jost B. , Fazio Massimo A. , Weinreb Robert N. , Zangwill Linda M. 
Published: April 2021

AbstractThis study characterizes differences in glaucomatous eyes with and without high axial myopia using custom automated analysis of OCT images. 452 eyes of 277 glaucoma patients were stratified into non (n = 145 eyes), mild (n = 214 eyes), and high axial myopia (axial length (AL) > 26 mm, n = 93 eyes). Optic disc ovality index, tilt and rotation angle of Bruch´s membrane opening (BMO) and peripapillary choroidal thickness (PCT) were calculated using automated and deep learning strategies. High myopic optic discs were more oval and had larger BMO tilt than mild and non-myopic discs (both p < 0.001). Mean PCT was thinnest in high myopic eyes followed by mild and non-myopic eyes (p < 0.001). BMO rotation angle, global retinal nerve fiber layer (RNFL) thickness and BMO-minimum rim width (MRW) were similar among groups. Temporal RNFL was thicker and supranasal BMO-MRW was thinner in high myopic eyes. BMO tilt and PCT showed moderate and temporal RNFL and nasal BMO-MRW showed weak but significant associations with AL in multivariable analyses (all p < 0.05). Large BMO tilt angle and thin PCT are characteristics of highly myopic discs and were not associated with severity of glaucoma. Caution should be exercised when using sectoral BMO-MRW and RNFL thickness for glaucoma management decisions in myopic eyes.
Modeling space radiation induced cognitive dysfunction using targeted and non-targeted effects
Authors: Shuryak Igor , Brenner David J. , Blattnig Steven R. , Shukitt-Hale Barbara , Rabin Bernard M. 
Published: April 2021

AbstractRadiation-induced cognitive dysfunction is increasingly recognized as an important risk for human exploration of distant planets. Mechanistically-motivated mathematical modeling helps to interpret and quantify this phenomenon. Here we considered two general mechanisms of ionizing radiation-induced damage: targeted effects (TE), caused by traversal of cells by ionizing tracks, and non-targeted effects (NTE), caused by responses of other cells to signals released by traversed cells. We compared the performances of 18 dose response model variants based on these concepts, fitted by robust nonlinear regression to a large published data set on novel object recognition testing in rats exposed to multiple space-relevant radiation types (H, C, O, Si, Ti and Fe ions), covering wide ranges of linear energy transfer (LET) (0.22–181 keV/µm) and dose (0.001–2 Gy). The best-fitting model (based on Akaike information criterion) was an NTE + TE variant where NTE saturate at low doses (~ 0.01 Gy) and occur at all tested LETs, whereas TE depend on dose linearly with a slope that increases with LET. The importance of NTE was also found by additional analyses of the data using quantile regression and random forests. These results suggest that NTE-based radiation effects on brain function are potentially important for astronaut health and for space mission risk assessments.
Ecological niche adaptation of Salmonella Typhimurium U288 is associated with altered pathogenicity and reduced zoonotic potential
Authors: Kirkwood Mark , Vohra Prerna , Bawn Matt , Thilliez Gaëtan , Pye Hannah , Tanner Jennifer , Chintoan-Uta Cosmin , Branchu Priscilla , Petrovska Liljana , Dallman Timothy , Hall Neil , Stevens Mark P. , Kingsley Robert A. 
Published: April 2021

AbstractThe emergence of new bacterial pathogens is a continuing challenge for agriculture and food safety. Salmonella Typhimurium is a major cause of foodborne illness worldwide, with pigs a major zoonotic reservoir. Two phylogenetically distinct variants, U288 and ST34, emerged in UK pigs around the same time but present different risk to food safety. Here we show using genomic epidemiology that ST34 accounts for over half of all S. Typhimurium infections in people while U288 less than 2%. That the U288 clade evolved in the recent past by acquiring AMR genes, indels in the virulence plasmid pU288-1, and accumulation of loss-of-function polymorphisms in coding sequences. U288 replicates more slowly and is more sensitive to desiccation than ST34 isolates and exhibited distinct pathogenicity in the murine model of colitis and in pigs. U288 infection was more disseminated in the lymph nodes while ST34 were recovered in greater numbers in the intestinal contents. These data are consistent with the evolution of S. Typhimurium U288 adaptation to pigs that may determine their reduced zoonotic potential.
Impact of advanced inverter functions on low‐voltage power grids
Authors: Mentens Arjen , Chamorro Harold R. , Jacobs Valéry Ann , Topolánek David , Drápela Jiří , Martinez Wilmar 
Published: April 2021

AbstractIn today's power grid, a great number of inverter‐based distributed energy resources (DERs) are connected and are mainly designed to supply power without considering the voltage and frequency deviations of the grid. Therefore, distribution system operators (DSOs) are challenged with an increase in grid events because of the random implementation of DERs. Voltage levels can vary beyond predefined limits at the point of connection and are currently not evaluated by DSOs. Summarized here is the development of a simulation model for evaluating the impact of support functions integrated in inverter‐based DERs. The model aims to help grid operators simulate voltage and frequency events and study the impact of DERs to the grid with respect to different settings of integrated support functions. A model is developed in MATLAB/Simulink conforming to European standards and regulations. Grid dynamics can be evaluated by imitating voltage and frequency deviations. Support functions can be either adjusted according to the situation or turned off. Together with adjustable settings according to DSO request, this model offers flexibility and insight in the capabilities of DERs to solve voltage and frequency issues. Case studies show that the model corresponds to expected behaviour and can be used for further development.