bluuebunny/crossref_metadata_2025_split huggingface dataset:
Oxidative stress tolerance contributes to heterologous protein production in Pichia pastoris
Authors: Lin Nai-Xin , He Rui-Zhen , Xu Yan , Yu Xiao-Wei 
Published: July 2021

Abstract
Background
Pichia pastoris (syn. Komagataella phaffii) is an important yeast system for heterologous protein expression. A robust P. pastoris mutant with oxidative and thermal stress cross-tolerance was acquired in our previous study. The robust mutant can express a 2.5-fold higher level of lipase than its wild type (WT) under methanol induction conditions.

Results
In this study, we found that the robust mutant not only can express a high level of lipase, but also can express a high level of other heterogeneous proteins (e.g., green fluorescence protein) under methanol induction conditions. Additionally, the intracellular reactive oxygen species (ROS) levels in the robust mutant were lower than that in the WT under methanol induction conditions. To figure out the difference of cellular response to methanol between the WT and the robust mutant, RNA-seq was detected and compared. The results of RNA-seq showed that the expression levels of genes related to antioxidant, MAPK pathway, ergosterol synthesis pathway, transcription factors, and the peroxisome pathway were upregulated in the robust mutant compared to the WT. The upregulation of these key pathways can improve the oxidative stress tolerance of strains and efficiently eliminate cellular ROS. Hence, we inferred that the high heterologous protein expression efficiency in the robust mutant may be due to its enhanced oxidative stress tolerance. Promisingly, we have indeed increased the expression level of lipase up to 1.6-fold by overexpressing antioxidant genes in P. pastoris.

Conclusions
This study demonstrated the impact of methanol on the expression levels of genes in P. pastoris and emphasized the contribution of oxidative stress tolerance on heterologous protein expression in P. pastoris. Our results shed light on the understanding of protein expression mechanism in P. pastoris and provided an idea for the rational construction of robust yeast with high expression ability.
Regression of taxane-related cystoid macular edema after topical dorzolamide treatment: two case reports
Authors: Otsubo Mitsuru , Kinouchi Reiko , Kamiya Takayuki , Yoshida Akitoshi 
Published: July 2021

Abstract
Background
Cystoid macular edema is a rare, vision-threatening side effect of the taxane family of anticancer agents. There is no established treatment or standard treatment protocol for taxane-related cystoid macular edema. Here, we report two cases of taxane-related cystoid macular edema that were treated with topical dorzolamide.

Case presentation
In case 1, a 72-year-old Japanese woman with bilateral geographic choroiditis reported for a follow-up visit with a complaint of blurred vision in both eyes for 2 months after starting nanoparticle albumin-bound paclitaxel chemotherapy for multiple metastases of her breast cancer. Her best-corrected visual acuity had dropped from 1.2 to 0.9 in the right eye and from 1.0 to 0.4 in the left eye. Fundus examination showed no newly active geographic choroiditis lesion, but optical coherence tomography exhibited cystoid macular edema. We suspected taxane-related cystoid macular edema and terminated nanoparticle albumin-bound paclitaxel, and started topical dorzolamide treatment. Cystoid macular edema nearly resolved within 6 weeks in the right eye and within 10 weeks in the left eye after starting topical dorzolamide treatment. The resolution of cystoid macular edema without leaving a chorioretinal scar after discontinuation of paclitaxel confirmed our initial diagnosis of taxane-related cystoid macular edema. A few inconspicuous cystoid spaces persisted at the parafovea for a year after dorzolamide treatment ended, but regressed after restarting dorzolamide treatment without any side effects. Best-corrected visual acuity improved to 1.2 in the right eye and 1.0 in the left eye. In case 2, a 70-year-old Japanese man, who received nanoparticle albumin-bound paclitaxel for pancreatic cancer with multiple metastases, developed bilateral cystoid macular edema. Best-corrected visual acuity was 0.3 bilaterally. Cystoid macular edema resolved within 5 weeks after stopping nanoparticle albumin-bound paclitaxel and starting topical dorzolamide treatment confirming the diagnosis of taxane-related cystoid macular edema. Nine weeks later, best-corrected visual acuity improved to 0.8 in the right eye and 1.0 in the left eye.

Conclusions
Cystoid macular edema in each case resolved within a few months without any side effects using topical dorzolamide and terminating taxane-based chemotherapy. Topical dorzolamide appears to be a safe and effective treatment option for patients with taxane-related cystoid macular edema whose quality of life is threatened by visual disturbances.
Severe reinfection with severe acute respiratory syndrome coronavirus 2 in a nursing home resident: a case report
Authors: Bader Nimrah , Khattab Mahmood , Farah Fahmi 
Published: July 2021

Abstract
Background
The topic of natural immunity related to severe acute respiratory syndrome coronavirus 2 remains controversial. Although evidence suggests postinfection immunity can be achieved, there have been reported cases of reinfection with similar or milder symptoms. Information on severe disease manifestation during reinfection is not known. We present a case of reinfection with a more severe presentation as compared with the initial infection.

Case Report
We describe a white male patient from a nursing home who was reinfected with severe acute respiratory syndrome coronavirus 2 with severe disease manifesting as dyspnea, fevers, and encephalopathy with hypoxemic respiratory failure requiring intubation, elevated inflammatory markers, and lung infiltrates on imaging, after initially testing positive with mild symptoms 2 months prior to presentation. Notably, severe acute respiratory syndrome coronavirus 2 antibodies were detected, which indicated this was a coronavirus disease 2019 reinfection. After treatment with remdesivir, dexamethasone, and convalescent plasma, he was subsequently extubated and discharged home after 2 weeks.

Conclusion
It is not clear whether an initial infection with severe acute respiratory syndrome coronavirus 2 and recovery provides prolonged immunity beyond 2 months. Furthermore, even if antibodies are present, it does not guarantee an attenuated course during reinfection. Therefore, vaccination plays an important role in prevention. Long-term cohort studies will be needed to study the factors behind reinfection.
Renal 99mTc-DMSA pharmacokinetics in pediatric patients
Authors: Plyku Donika , Ghaly Michael , Li Ye , Brown Justin L. , O’Reilly Shannon , Khamwan Kitiwat , Goodkind Alison B. , Sexton-Stallone Briana , Cao Xinhua , Zurakowski David , Fahey Frederic H. , Treves S. Ted , Bolch Wesley E. , Frey Eric C. , Sgouros George 
Published: July 2021

Abstract
99mTc-DMSA is one of the most commonly used pediatric nuclear medicine imaging agents. Nevertheless, there are no pharmacokinetic (PK) models for 99mTc-DMSA in children, and currently available pediatric dose estimates for 99mTc-DMSA use pediatric S values with PK data derived from adults. Furthermore, the adult PK data were collected in the mid-70’s using quantification techniques and instrumentation available at the time. Using pediatric imaging data for DMSA, we have obtained kinetic parameters for DMSA that differ from those applicable to adults.

Methods
We obtained patient data from a retrospective re-evaluation of clinically collected pediatric SPECT images of 99mTc-DMSA in 54 pediatric patients from Boston’s Children Hospital (BCH), ranging in age from 1 to 16 years old. These were supplemented by prospective data from twenty-three pediatric patients (age range: 4 months to 6 years old).

Results
In pediatric patients, the plateau phase in fractional kidney uptake occurs at a fractional uptake value closer to 0.3 than the value of 0.5 reported by the International Commission on Radiological Protection (ICRP) for adult patients. This leads to a 27% lower time-integrated activity coefficient in pediatric patients than in adults. Over the age range examined, no age dependency in uptake fraction at the clinical imaging time was observed. Female pediatric patients had a 17% higher fractional kidney uptake at the clinical imaging time than males (P < 0.001).

Conclusions
Pediatric 99mTc-DMSA kinetics differ from those reported for adults and should be considered in pediatric patient dosimetry. Alternatively, the differences obtained in this study could reflect improved quantification methods and the need to re-examine DMSA kinetics in adults.
Passive multichannel analysis of surface waves using 1D and 2D receiver arrays
Authors: Morton Sarah L. , Ivanov Julian , Peterie Shelby L. , Miller Richard D. , Livers-Douglas Amanda J. 
Published: July 2021

We use the seismic multichannel analysis of surface waves (MASW) method, which originally used active sources (i.e., controlled by users), to present a new alternative technique for surface-wave analysis of passive data (i.e., seismic sources not controlled by users). Our passive MASW technique uses two types of receiver arrays, 1D and 2D, for two different purposes. The 2D square array is used for assessing the number and directionality of passive seismic sources to help identify optimal time window(s) when such sources are of sufficient quality and aligned with the 1D receiver spread. Then, these time windows are used to selectively extract passive seismic data recorded by the 1D receiver spread for subsequent MASW analysis. In such a manner, this hybrid 1D–2D passive MASW approach can approximate the higher quality advantages of the active MASW method. This technique is successfully applied at a test site in southcentral Kansas taking advantage of train energy as the passive sources monitoring and assessing the potential development of vertically migrating voids that could endanger groundwater and infrastructure. The 1D–2D passive MASW method has proven beneficial for optimizing passive-data dispersion patterns and accurately assessing relative velocities for investigations with reduced labor costs and minimal added processing time and can be considered as a contribution to the surface-wave analysis tools.
Salivary Gland Hypofunction and/or Xerostomia Induced by Nonsurgical Cancer Therapies: ISOO/MASCC/ASCO Guideline
Authors: Mercadante Valeria , Jensen Siri Beier , Smith Derek K. , Bohlke Kari , Bauman Jessica , Brennan Michael T. , Coppes Robert P. , Jessen Niels , Malhotra Narinder K. , Murphy Barbara , Rosenthal David I. , Vissink Arjan , Wu Jonn , Saunders Deborah P. , Peterson Douglas E. 
Published: July 2021

PURPOSE To provide evidence-based recommendations for prevention and management of salivary gland hypofunction and xerostomia induced by nonsurgical cancer therapies. METHODS Multinational Association of Supportive Care in Cancer/International Society of Oral Oncology (MASCC/ISOO) and ASCO convened a multidisciplinary Expert Panel to evaluate the evidence and formulate recommendations. PubMed, EMBASE, and Cochrane Library were searched for randomized controlled trials published between January 2009 and June 2020. The guideline also incorporated two previous systematic reviews conducted by MASCC/ISOO, which included studies published from 1990 through 2008. RESULTS A total of 58 publications were identified: 46 addressed preventive interventions and 12 addressed therapeutic interventions. A majority of the evidence focused on the setting of radiation therapy for head and neck cancer. For the prevention of salivary gland hypofunction and/or xerostomia in patients with head and neck cancer, there is high-quality evidence for tissue-sparing radiation modalities. Evidence is weaker or insufficient for other interventions. For the management of salivary gland hypofunction and/or xerostomia, intermediate-quality evidence supports the use of topical mucosal lubricants, saliva substitutes, and agents that stimulate the salivary reflex. RECOMMENDATIONS For patients who receive radiation therapy for head and neck cancer, tissue-sparing radiation modalities should be used when possible to reduce the risk of salivary gland hypofunction and xerostomia. Other risk-reducing interventions that may be offered during radiation therapy for head and neck cancer include bethanechol and acupuncture. For patients who develop salivary gland hypofunction and/or xerostomia, interventions include topical mucosal lubricants, saliva substitutes, and sugar-free lozenges or chewing gum. For patients with head and neck cancer, oral pilocarpine and oral cevimeline, acupuncture, or transcutaneous electrostimulation may be offered after radiation therapy. Additional information can be found at www.asco.org/supportive-care-guidelines .
Quality Care in Survivorship: Lessons Learned From the ASCO Quality Oncology Practice Initiative
Authors: Shapiro Charles L. , Zubizarreta Nicole , Moshier Erin , Brockway Julia P. , Mandeli John , Markham Merry Jennifer , Kozlik Mary May , Crist Stephanie , Jacobsen Paul B. 
Published: July 2021

PURPOSE: The ASCO Quality Oncology Practice Initiative (QOPI) project was established to evaluate the influence of guideline recommendations on routine clinical practice. METHODS: QOPI provided summary data from 839 unique practices in which data were collected every six months from the Fall of 2015 to the Spring of 2019. From these data, six items were chosen based on their relationship to domains of survivorship. A zero-inflated negative binomial regression model was used to test for trends in QOPI measures adherence rates over time. The models were adjusted for the time period, region, practice-ownership, multispecialty site, fellowship program, and hospital type. RESULTS: Smoking cessation counseling recommended and smoking cessation counseling administered or referred both increased over time, 50%-61% (adjusted incidence rate ratios (IRR), 1.028; 95% CI, 1.016 to 1.040; P < .001) and 34%-49% (adjusted IRR, 1.052; 95% CI, 1.035 to 1.070; P < .001), respectively. Infertility risks discussed before chemotherapy increased from 36% to 53% (adjusted IRR, 1.056; 95% CI, 1.035 to 1.078; P < .001) and fertility options discussed or referred to specialists increased from 23% to 38% (adjusted IRR, 1.074; 95% CI, 1.046 to 1.102; P < .001). Twenty-nine percent documented a positron emission tomography, computed tomography, or bone scan within the first 12 months for women diagnosed with early breast cancer treated for curative intent (adjusted IRR, 1.000; 95% CI, 0.977 to 1.024; P = .971). Tumor marker surveillance within 12 months increased from 78% to 87% (adjusted IRR, 1.018; 95% CI, 1.002 to 1.033; P = .023). CONCLUSION: As scientific evidence to guide cancer survivorship care grows, the role of guideline recommendations permeating clinical practice using quality metrics will become increasingly important.
From tip to toe – dressing centrioles in γTuRC
Authors: Schweizer Nina , Lüders Jens 
Published: July 2021

ABSTRACT
Centrioles are microtubule-based cylindrical structures that assemble the centrosome and template the formation of cilia. The proximal part of centrioles is associated with the pericentriolar material, a protein scaffold from which microtubules are nucleated. This activity is mediated by the γ-tubulin ring complex (γTuRC) whose central role in centrosomal microtubule organization has been recognized for decades. However, accumulating evidence suggests that γTuRC activity at this organelle is neither restricted to the pericentriolar material nor limited to microtubule nucleation. Instead, γTuRC is found along the entire centriole cylinder, at subdistal appendages, and inside the centriole lumen, where its canonical function as a microtubule nucleator might be supplemented or replaced by a function in microtubule anchoring and centriole stabilization, respectively. In this Opinion, we discuss recent insights into the expanded repertoire of γTuRC activities at centrioles and how distinct subpopulations of γTuRC might act in concert to ensure centrosome and cilia biogenesis and function, ultimately supporting cell proliferation, differentiation and homeostasis. We propose that the classical view of centrosomal γTuRC as a pericentriolar material-associated microtubule nucleator needs to be revised.
Radiation Protection of Personnel in Contact Radiation Therapy in Ophthalmology
Authors: Kashirina O. , Timofeev L , Likhvantseva V. 
Published: July 2021

Purpose: To ensure radiation safety of medical staff personal protective equipment (PPE).
Material and methods: In order to not make assumptions of possible protective materials optical distortion, estimated the dependence of light transmission in the wavelength range 330–660 nm leaded glass brands of TF-1 and TF-5, as well as the intensity of glow glasses when exposed to light and X-rays.
Results: We trace the degree of browning and the nature of the recovery of transparency of glass after irradiation. To determine the optimum thickness of lead glass for eyewear experimentally evaluated attenuation of X-rays with energy efficiency 30 and 80 keV. Lead equivalent values for lead glass, was determined so for Eeff ~ 20 keV at a multiplicity of weakening k = 10 lead glass brand TF-5 thickness 2.0 mm equivalent 0.8 mm Pb, etc. For the possible use of other additives in the window shows the curves of the attenuation of photon radiation radionuclide 241Am (20–60 keV) filters from 9Be, 26Al, 56Fe, 64Cu, 99Mo, 112Cd, 184W, 207Pb
Conclusion: Possible introduction of new dose limits for the lens of the eye can be successful only in case of both existing and newly developed PPE. To reduce the impact of domestic lead glass radiation can be used for staff in the form of screens and glasses.
Analysis of the Physical and Radiobiological Equivalence of the Calculated and Measured Dose Distributions for Prostate Stereotactic Radiotherapy
Authors: Sukhikh E. , Sukhikh L. , Vertinsky A. , Izhevsky P. , Sheino I. , Vertoukhova V. 
Published: July 2021

Purpose: Carrying out the analysis of the physical and radiobiological equivalence of dose distributions obtained during the planning of hypofractionated stereotactic radiation therapy of the prostate cancer and verification using a three-dimensional cylindrical dosimeter.
Material and Methods: Based on the anatomical data of twelve patients diagnosed with prostate carcinoma, stage T2N0M0 with low risk, plans were developed for stereotactic radiation therapy with volumetric modulates arc therapy (VMAT). The dose per fraction was 7,25 Gy for 5 fractions (total dose 36,25 Gy) with a normal photon energy of 10 MV. The developed plans were verified using a three-dimensional cylindrical ArcCHECK phantom. During the verification process, the three-dimensional dose distribution in the phantom was measured, based on which the values of the three-dimensional gamma index and the dose–volume histogram within each contoured anatomical structures were calculated with 3DVH software.
The gamma index value γ (3 %, 2 mm, GN) at a threshold equal to 20 % of the dose maximum of the plan and the percentage of coincidence of points at least 95 % was chosen as a criterion of physical convergence of the calculated and measured dose distribution according to the recommendations of AAPM TG-218. To analyze the radiobiological equivalence of the calculated and measured dose distribution, the local control probability (TCP) and normal tissue complication probability (NTCP) criteria were used based on the calculated and measured dose–volume histograms. Contours of the target (PTV) and the anterior wall of the rectum were used for the analysis. The approach based on the concept of equivalent uniform dose (EUD) by A. Niemierko was used to calculate the values of TCP/NTCP criteria.
Results: The results of physical convergence of plans for all patients on the contour of the whole body were higher than 95 % for the criteria γ (3 %, 2 mm, GN). The convergence along the PTV contour is in the range (75.5–95.2)%. The TCP and NTCP values obtained from the measured dose-volume histograms were higher than the planned values for all patients. It was found that the accelerator delivered a slightly higher dose to the PTV and the anterior wall of the rectum than originally planned.
Conclusion: The capabilities of modern dosimetric equipment allow us move to the verification of treatment plans based on the analysis of TCP / NTCP radiobiological equivalence, taking into account the individual characteristics of the patient and the capabilities of radiation therapy equipment.
Modern Treatment of Non-Small Cell Lung Cancer with Intracerebral Metastases and Extracranial Metastatic Affection of Liver, Mediastinal Lymph Nodes, Skeletal Bones. Clinical Observation
Authors: Udalov Yu. , Samoylov A. , Danilova L. , Slobina E. , Stepanyuchenko E. , Bogomolova I. , Kashentseva N. , Kiselev V. , Vanchugov A. , Andreev V. , Stepanov E. 
Published: July 2021

Introduction: The article covers issues related to modern methods of treatment of non-small cell lung cancer (NSCLC) with multiple synchronous metastases in brain, liver, mediastinal lymph nodes, skeletal bones and describes a clinical case of modern effective treatment and long-term follow-up of a patient with advanced NSCLC and extra- and intracranial metastatic spread.
Purpose: Evaluation of modern methods of special antitumor treatment and their application in clinical practice for the treatment of metastatic NSCLC.
Material and methods: Modern methods of proton and photon radiation therapy, targeted therapy are considered.
Results: The use of modern methods of special antitumor therapy has increased the overall and relapse-free survival rate of patients with multiple metastases of NSCLC in brain, liver, mediastinal lymph nodes, skeletal bones reducing the need for additional interventions. This is confirmed by a long-term follow-up after modern effective antitumor treatment of a patient with advanced NSCLC and synchronous progression in the form of extra- and intracranial metastatic spread. The patient has been alive for more than 2 years from the moment of progression with an estimated life expectancy of 4–5 months.
Conclusion: Modern methods of special antitumor therapy can increase the survival rate of patients with multiple synchronous NSCLC metastases in brain, liver, mediastinal lymph nodes, skeletal bones without significant deterioration in their quality of life.
RiRiShun Logistics: Home Appliance Delivery Data for the 2021 Manufacturing & Service Operations Management Data-Driven Research Challenge
Authors: Guo Xiaolong , Yu Yugang , Allon Gad , Wang Meiyan , Zhang Zhentai 
Published: July 2021

To support the 2021 Manufacturing & Service Operations Management (M&SOM) Data-Driven Research Challenge, RiRiShun Logistics (a Haier group subsidiary focusing on logistics service for home appliances) provides MSOM members with logistics operational-level data for data-driven research. This paper provides a detailed description of the data associated with over 14 million orders from 149 clients (the consigners) associated with 4.2 million end consumers (the recipients and end users of the appliances) in China, involving 18,000 stock keeping units operated at 103 warehouses. Researchers are welcomed to develop econometric models, data-driven optimization techniques, analytical models, and algorithm designs by using this data set to address questions suggested by company managers. History: This paper has been accepted as part of the 2021 M&SOM Data-Driven Research Challenge. Funding: This work was supported by the National Natural Science Foundation of China [Grants 72091215/72091210, 71871207, 71991464/71991460, and 71921001]. Supplemental Material: Data and the online appendix are is available at https://doi.org/10.1287/msom.2021.0994 .