bluuebunny/crossref_metadata_2025_split huggingface dataset:
Transcriptomic-based toxicological investigations of graphene oxide with modest cytotoxicity to human umbilical vein endothelial cells: changes of Toll-like receptor signaling pathways
Authors: Luo Yingmei , Wang Xuefeng , Cao Yi 
Published: September 2021

Abstract
The wide uses of graphene oxide (GO) lead to the contact of GO with vascular systems, so it is necessary to investigate the toxicological effects of GO to endothelial cells. Recently, we reported that GO of small lateral size (&lt;500 nm) was relatively biocompatible to human umbilical vein endothelial cells (HUVECs), but recent studies by using omics-techniques revealed that nanomaterials (NMs) even without acute cytotoxicity might induce other toxicological effects. This study investigated the effects of GO on HUVECs based on RNA-sequencing and bioinformatics analysis. Even after exposure to 100 μg/ml GO, the cellular viability of HUVECs was higher than 70%. Furthermore, 25 μg/ml GO was internalized but did not induce ultrastructural changes or intracellular superoxide. These results combined indicated GO’s relatively high biocompatibility. However, by analyzing the most significantly altered Gene Ontology terms and Kyoto Encyclopedia of Gene and Genomes pathways, we found that 25 μg/ml GO altered pathways related to immune systems’ functions and the responses to virus. We further verified that GO exposure significantly decreased Toll-like receptor 3 and interleukin 8 proteins, indicating an immune suppressive effect. However, THP-1 monocyte adhesion was induced by GO with or without the presence of inflammatory stimulus lipopolysaccharide. We concluded that GO might inhibit the immune responses to virus in endothelial cells at least partially mediated by the inhibition of TLR3. Our results also highlighted a need to investigate the toxicological effects of NMs even without acute cytotoxicity by omics-based techniques.
Biologic Reconstruction With a Motorized Intramedullary Bone Transport Nail After Tumor Resection
Authors: Zuckerman Lee M. 
Published: September 2021

Summary:
Distraction osteogenesis is an option for reconstruction of intercalary defects. The use of bone transport after tumor surgery has been limited because of concerns of pin tract infections with external fixation and the theoretical risk of causing tumor growth. The effects of chemotherapy and radiation on the regenerate and healing of the docking site are also not well studied, but the current literature has mostly favorable outcomes with no evidence of causing tumor proliferation. The Precice bone transport nail offers a noninvasive method of distraction osteogenesis, which eliminates the need for prolonged external fixation and the risk of pin tract infections. This report discusses the technique for using the Precice bone transport nail after tumor resection. Bone transport may be considered for intercalary defects after en bloc resection of sarcoma, metastatic disease, and benign aggressive bone tumors. The use of distraction osteogenesis after tumor resection is a promising technique for the biologic reconstruction of intercalary defects.
Management of Segmental Tibial Bone Defects With a Motorized Intramedullary Bone Transport Nail: A Case Review With Follow-Up
Authors: Stoneback Jason W. , Erdman Mary Kate , Marecek Geoffrey S. 
Published: September 2021

Summary:
Large segment bone defects of the tibia are challenging problems. Although caused by a wide range of conditions, tibial critical bone loss defects often require complex reconstructive plans with prolonged inability to weight-bear on the effected extremity. Reconstruction options frequently require harvesting of autograft leading to further morbidity. Distraction osteogenesis allows reconstruction of large segmental defects of the tibia while avoiding donor site morbidity. Historically, distraction osteogenesis of tibia was most reliably performed with circler ring external fixation. This process allowed early weight-bearing but unfortunately has considerable drawbacks. Negative effects include pin tract irritation and inability to wear normal clothes. The advent of the bone transport nail now allows management of tibial critical bone loss defects through distraction osteogenesis negating the need for external fixation. This new technique allows treatment of large segmental tibial defects by means of distraction osteogenesis with an all-internal device avoiding the negative effects of external fixation while simultaneously allowing early weight-bearing.
Geriatric Acetabular Fractures and Femoral Head Protrusio: Methods of Fixation and the Option of Total Hip Arthroplasty
Authors: Walters Murphy M. , Bergin Patrick F. , Russell George V. , Morellato John T. 
Published: September 2021

Summary:
Currently, the literature is unclear regarding the optimal treatment algorithm for geriatric acetabular fractures. In a recent epidemiological study, 70% of all acetabular fractures in patients older than 65 years were classified as either associated both column or anterior column/posterior hemitransverse. Within the subset of these fractures, variants with significant femoral head protrusio, which is defined as the displacement of the femoral head medial to the ilioischial line, present with unique challenges. Goals of treatment in these cases should include surgical techniques that minimize the physiologic insult for the patient yet restore hip congruity and stability. “Fix and replace” is becoming an increasingly popular approach in the acute setting; however, its indications are not yet well-established. At our institution, we often favor open reduction and internal fixation alone as an effective and efficient way to treat the protrusio variant, even at the expense of protected weight-bearing. In the following article, we present a systematic approach for the management of geriatric acetabular fracture femoral head protrusio along with specific case examples.
Geriatric Ankle Fracture: Robust Fixation Versus Hindfoot Nail
Authors: Srinath Arjun , Matuszewski Paul E. , Kalbac Tyler 
Published: September 2021

Summary:
The incidence of geriatric ankle fractures is increasing in step with the current aging population. Special considerations must be taken into account when treating geriatric patients due to increased medical comorbidities, worse soft tissue envelope, poor bone quality, and the needs for early mobilization. The orthopaedic surgeon must have a variety of surgical options to treat fractures in the elderly population based on patient-specific needs. This article uses a case-based approach to discuss relevant considerations when selecting between hindfoot fusion nail and robust open reduction and internal fixation for geriatric ankle fractures.
Characterizing Adverse Events Reporting for an Over-the-Counter Disposable Intravaginal Support Device for Stress Urinary Incontinence
Authors: Sansone Stephanie A. , Stoddard Michelina D. , Cho Ahra , Asfaw Tirsit S. , Sedrakyan Art , Chughtai Bilal 
Published: September 2021

Objectives
In recent years, new intravaginal support devices for stress urinary incontinence have been introduced to the market. Although studies have demonstrated their safety and efficacy, few studies have examined adverse events attributed to their use. The objective of this study was to characterize reported adverse events for an over-the-counter intravaginal support device and determine whether further medical treatment was necessary to manage the adverse event.

Methods
We searched the Manufacturer and User Device Experience database from January 2015 to September 2020 for pertinent reported adverse events, which included the unique report key, event date, report date, event text descriptions, and resulting course of treatment. Per Manufacturer and User Device Experience database mandatory requirements, all reports were submitted by the manufacturer within 30 days of receiving the consumer’s voluntary submission.

Results
A total of 257 reports were included for analysis. The most common reported adverse event was related to the string of the device, which broke with either removal or insertion of the device (230 of 257 [89.5%]). The majority of patients who required medical attention (133 of 257 [51.8%]) were evaluated and managed in the office setting (85 of 133 [63.9%]), whereas 37% (95 of 257) of patients were able to self-treat.

Conclusions
Most complications attributed to device use were self-managed or managed in the outpatient setting. No emergent hospitalizations or deaths were attributed to their use, supporting their generally low-risk profile and high tolerability. Limitations of this review include the inability to decipher a denominator for these reported adverse events and the potential bias attributed to voluntary consumer reporting.
Levator-Urethra Gap: Normative Data in a Nonpregnant Nulliparous Population
Authors: Gregory W. Thomas , Cramer Meagan , Holland Amanda , Boniface Emily 
Published: September 2021

Objective
This study aimed to establish a normative range of the levator-urethra gap (LUG) measurement in nonpregnant, nulliparous women to be used as a standard against which assessments of injury or avulsion of the levator ani could be made.

Methods
Nulliparous women in the Pacific Northwest who were planning pregnancy within the ensuing 12 months underwent high-resolution magnetic resonance imaging (MRI) and 3-dimensional transperineal ultrasonography. In the plane of minimal hiatal dimensions, the LUG was measured on both the right and left sides for 3 contiguous image slices.

Results
One hundred thirty-five participants underwent examinations, of which 134 had evaluable MRIs and 70 had evaluable ultrasounds. Mean (SD) age was 30.8 (4.0) years, and mean (SD) body mass index was 25.9 (6.4). The majority of the participants self-identified as White (80%) and non-Hispanic (89%). The 99th percentile was no greater than 2.39 cm on any slice for either MRI or US-based measurements. In the MRI set, the lowest LUG values at which all 3 measurements on a side were found to have exceeded a putative threshold value (for injury) were 2.1 cm for the right side and 2.05 cm for the left side. The corresponding LUG values in the ultrasound set were 2.2 cm on the right and 2.25 cm on the left.

Conclusions
The LUG threshold in 3 successive slices in the minimal hiatal dimension suggestive of levator ani injury could be as low as 2.1 cm when using MRI and 2.25 cm when using ultrasound in a similar population.
Conducive environments reduce sleep disturbances and improve sleep quality: a quality improvement project
Authors: Ho Alexander G.T. , Tan Mary , Perez Hilario M. , Tan Ching Nee. , Mordiffi Siti Z. 
Published: September 2021

ABSTRACT

Aim:
The current implementation project aimed to improve patients’ sleep quality in a hospital by implementing targeted interventions to reduce sleep disturbances.

Methods:
The Silent Night project was implemented in two general units of an academic tertiary hospital. The project comprised three phases: preimplementation, implementation, and postimplementation. Patient surveys were administered to obtain patients’ perspectives on the sources of noise disturbances at night. Noise-monitoring machines were installed in two wards to obtain objective data on noise levels at night. Concurrently, data were collected on noise-generating activities that were observed.

Results:
The overall quality of sleep reported by patients improved by 17% (from 73.5 to 88.9%). Patients’ survey reported reduced noise disturbances from direct care activities, environmental noise and medical equipment alarms. The mean noise level in the orthopedics unit reduced significantly from 57.04 to 55.22 dB with a corresponding decrease in noise generating activities by 60% (from 362 to 144). In the geriatric unit, the mean noise level increased from 51.36 to 53.12 dB but is within the National Environmental Agency's permissible noise level of 55 dB even though the noise generating activities reduced significantly by 92.2% (from 954 to 74).

Conclusion:
The ‘Silent Night’ project has successfully reduced noise disturbances generating from environment and nursing care activities and improved patients’ sleep quality in the hospital. Sharing sessions regarding sources of noise and the ‘Silent Night’ poster promoted the awareness of noise disturbances in healthcare professionals, visitors, and patients.
The equivalence of hydration status measured by the Fresenius BCM and the Cella Bioimpedance Spectroscopy devices on hemodialysis patients
Authors: Matthie Jim , Baricevic Borut , Malnaric Marentic Vlasta , Krajacic Boris 
Published: September 2021

AbstractBackgroundFluid management is a serious challenge for patients undergoing hemodialysis therapy (HD). Bioimpedance spectroscopy (BIS) is a promising technique to help with clinical hydration (HYD) assessment. The Fresenius Medical Care (FMC) Body Composition Monitor (BCM) is the standard but is large and expensive. Cella Medical has introduced a small wireless BIS device. This study compared the HYD status predicted by the two devices.MethodsFollowing the FMC BCM device manual guidelines, measurements of BIS were made wrist-ankle using typical ECG electrodes on the non-fistula side of HD patients pre dialysis while in their normal supine position. As usual, patients were measured before their normal time of therapy with the BCM. The Cella measurements were then performed within two minutes.ResultsForty-two HD patients (M=64%, age=64±30 yrs.), were measured. One patient data was removed. The mean BCM HYD status was 1.86 l, SD 1.46 l, and SEM 0.22 l. Cella was 1.806 l, SD 1.36 l, and the SEM 0.21 l. The 95% difference confidence interval (CI) was -0.66 to 0.55 l. The Pearson’s correlation (r) was r^2 = 0.85 (p<0.00001). There was no proportional bias: the offset was -0.056 l, and K=1.010. The limits of agreement (LOA) analysis showed a mean difference of 0.56 l, and limits d ±2SD = (−1.192 l, 1.081 l), indicating 95% of the difference will lie within these limits. To evaluate equivalence, we performed two one-sided t-tests (TOST). When the bounds were reduced to the limit =0.47 l and -0.59 l, we obtained a 0.046 p-value (alpha =0.05), at 80% statistical power. For 26% of the subjects, the difference was <0.1 l, for 43% <0.25 l, for 71% <0.5 l, for 83% <0.75 l, for 90% <1.0 l, and for 9.5% (4 patients) more than 1 l. Only two cases (4.8%) were just over the ±2SD limit.ConclusionThis study suggests the BCM and Cella devices can be used interchangeably.
Seroresponse to SARS-CoV-2 vaccines among maintenance dialysis patients over six months
Authors: Hsu Caroline M. , Weiner Daniel E. , Manley Harold J. , Aweh Gideon N. , Ladik Vladimir , Frament Jill , Miskulin Dana , Agyropoulos Christos , Abreo Kenneth , Chin Andrew , Gladish Reginald , Salman Loay , Johnson Doug , Lacson Eduardo K. 
Published: September 2021

AbstractBackground and ObjectivesWhile most maintenance dialysis patients exhibit initial seroresponse to vaccination, concerns remain regarding the durability of this antibody response. This study evaluated immunity over time.Design, setting, participants, and measurementsThis retrospective cohort study included maintenance dialysis patients from a midsize national dialysis provider who received a complete SARS-CoV-2 vaccine series and had at least one antibody titer checked after full vaccination. Immunoglobulin G spike antibodies (SAb-IgG) titers were assessed monthly with routine labs beginning after full vaccination and followed over time; the semiquantitative SAb-IgG titer reported a range between 0 and ≥ 20 U/L. Descriptive analyses compared trends over time by prior history of COVID-19 and type of vaccine received. Time-to-event analyses were conducted for the outcome of loss of seroresponse (SAb-IgG < 1 U/L or development of COVID-19). Cox proportional hazards regression was used to adjust for additional clinical characteristics of interest.ResultsAmong 1898 maintenance dialysis patients, 1567 (84%) had no prior history of COVID-19. Patients without a history of COVID-19 had declining titers over time. Among 441 BNT162b2/Pfizer recipients, median [IQR] SAb-IgG titer declined from 20 [5.99-20] U/L in month 1 to 1.30 [0.15-3.59] U/L by month 6. Among 779 mRNA-1273/Moderna recipients, median [IQR] SAb-IgG titer declined from 20 [20-20] in month 1 to 6.20 [1.74-20] by month 6. The 347 Ad26.COV2.S/Janssen recipients had a lower titer response than mRNA vaccine recipients over all time periods. In time-to-event analyses, Ad26.COV2.S/Janssen and mRNA-1273/Moderna recipients had the shortest and longest time to loss of seroresponse, respectively. The maximum titer reached in the first two months after full vaccination was predictive of the durability of the SAb-IgG seroresponse; patients with SAb-IgG titer 1-19.99 U/L were more likely to have loss of seroresponse compared to patients with SAb-IgG titer ≥ 20 U/L (HR 23.9 [95% CI: 16.1-35.5]).ConclusionsVaccine-induced seroresponse wanes over time among maintenance dialysis patients across vaccine types. Early titers after full vaccination predict the durability of seroresponse.
Metabotropic group II glutamate receptors mediate cue-triggered increases in incentive motivation for reward
Authors: Garceau Caroline , Marsault Justine , Robinson Mike J F , Samaha Anne-Noël 
Published: September 2021

AbstractRationaleReward-associated cues can acquire incentive motivational properties and invigorate reward-seeking actions via Pavlovian-to-instrumental transfer (PIT). Glutamatergic neurotransmission mediates the appetitive effects of reward-associated cues. We characterized the expression of PIT and its mediation by metabotropic group II glutamate (mGlu2/3) receptor activity in female and male rats.ObjectivesAcross the sexes, we used PIT procedures to determine i) cue-triggered increases in incentive motivation for water reward (Experiment 1), ii) the respective influences of the mGlu2/3 receptor agonist LY379268 and reward devaluation by satiation on this effect (Experiment 2).MethodsWater-restricted male and female Sprague-Dawley rats learned to lever press for water. Separately, they learned that one of two auditory stimuli predicts free water (CS+ vs CS-). On PIT test days, the CS+ and CS- were presented non-contingently, and we measured effects on lever pressing under extinction (no water). In Experiment 1, we characterized PIT across the sexes. In Experiment 2, we measured PIT after systemic LY379268 administration (0, 0.3 and 1 mg/kg), and water satiation, respectively.ResultsFemale and male rats showed similar PIT, with CS+ but not CS- presentations potentiating water-seeking behaviour. LY379268 (1 mg/kg) attenuated CS+ evoked increases in both water-associated lever pressing and conditioned approach to the water port. Reward devaluation attenuated both water-seeking and CS+ evoked conditioned approach behaviour.ConclusionsThe sexes show similar cue-triggered increases in reward ‘wanting’, and water devaluation suppresses both water seeking and cue-triggered anticipation of water reward. Finally, across the sexes, mGlu2/3 receptor activity mediates cue-triggered increases in reward ‘wanting’.
Immunoglobulin enhancers increase RNA polymerase 2 stalling at somatic hypermutation target sequences
Authors: Tarsalainen Alina , Maman Yaakov , Meng Fei-Long , Kyläniemi Minna K. , Soikkeli Anni , Budzynska Paulina , McDonald Jessica J. , Šenigl Filip , Alt Frederic W. , Schatz David G. , Alinikula Jukka 
Published: September 2021

ABSTRACTSomatic hypermutation (SHM) drives the genetic diversity of immunoglobulin (Ig) genes in activated B cells and supports the generation of antibodies with increased affinity for antigen. SHM is targeted to Ig genes by their enhancers (DIVACs; diversification activators), but how the enhancers mediate this activity is unknown. We show using chicken DT40 B cells that highly active DIVACs increase the phosphorylation of RNA polymerase 2 (Pol2) and Pol2 occupancy in the mutating gene with little or no accompanying increase in elongation-competent Pol2 or production of full-length transcripts, indicating accumulation of stalled Pol2. DIVAC has similar effect also in human Ramos Burkitt lymphoma cells. The DIVAC-induced stalling is weakly associated with an increase in the detection of single-stranded DNA bubbles in the mutating target gene. We did not find evidence for antisense transcription, or that DIVAC functions by altering levels of H3K27ac or the histone variant H3.3 in the mutating gene. These findings argue for a connection between Pol2 stalling and cis-acting targeting elements in the context of SHM and thus define a mechanistic basis for locus-specific targeting of SHM in the genome. Our results suggest that DIVAC elements render the target gene a suitable platform for AID-mediated mutation without a requirement for increasing transcriptional output.
Large-scale allosteric switch in the 7SK RNA regulates transcription in response to growth and stress
Authors: Olson Samuel W. , Turner Anne-Marie W. , Arney J. Winston , Saleem Irfana , Weidmann Chase A. , Margolis David M. , Weeks Kevin M. , Mustoe Anthony M. 
Published: September 2021

Summary7SK is a highly conserved non-coding RNA that regulates eukaryotic transcription by sequestering positive transcription elongation factor b (P-TEFb). 7SK regulatory function likely entails changes in RNA structure, but characterizing dynamic RNA-protein complexes in cells has remained an unsolved challenge. We describe a new chemical probing strategy (DANCE-MaP) that uses maximum likelihood deconvolution and probabilistic read assignment to define simultaneously (i) per-nucleotide reactivity profiles, (ii) direct base pairing interactions, and (iii) tertiary and higher-order interactions for each conformation of multi-state RNA structural ensembles, all from a single experiment. We show that human 7SK RNA, despite significant heterogeneity, intrinsically codes for a large-scale structural switch that couples dissolution of the P-TEFb binding site to structural remodeling at distal release factor binding sites. The 7SK structural equilibrium is regulated by cell type, shifts dynamically in response to cell growth and stress, and can be exogenously targeted to modulate transcription in cells. Our data support that the 7SK structural ensemble functions as an integrator of diverse cellular signals to control transcription elongation in environment and cell specific ways, and establishes DANCE-MaP as a powerful strategy for comprehensively defining RNA structure and dynamics in cells.
It is not a man's world: perceptions by male faculty of the status and representation of their female colleagues
Authors: Mousa Mohamed 
Published: September 2021

PurposeBy studying four public universities in Egypt, the author of this paper aims to identify how male faculty perceive the representation and status of their female colleagues.Design/methodology/approachThe author employed a qualitative research method via semi-structured interviews with 40 male academics in addition to five focus group discussions with an additional 20 male academics. The author subsequently used thematic analysis to determine the main ideas in the transcripts.FindingsThe findings confirmed that women faculty are not under-represented at professorial levels, but they are denied administrative academic positions, such as rectors and deans in universities. The author also discovered that the social norms shaping both national and organizational culture in Egypt create a cultural bias against women faculty.Originality/valueTo the best of the author's knowledge, this study is the first of its kind in the context of a developing nation to focus on the status and representation of women faculty from the perspective of male colleagues, and subsequently, it is the first to address the higher education sector in one of the leading developing nations in Africa and the Middle East. This paper contributes by filling a gap in HR management and higher education, in which empirical studies that address male faculty to identify their perceptions of the status and representation of their female colleagues have been limited so far.