bluuebunny/crossref_metadata_2025_split huggingface dataset:
FDH Knockout and TsFDH Transformation Led to Enhanced Growth Rate of Escherichia Coli
Authors: Razavipour Roya , Ashtiani Saman Hosseini , Sepahy Abbas Akhavan , Modarressi Mohammad Hossein , Bambai Bijan 
Published: December 2021

Abstract
Background:Increased Atmospheric CO2 to over 400 ppm has prompted global climate irregularities. Reducing the released CO2 from biotechnological processes could remediate these phenomena. In this study, we sought to find a solution to reduce the amount of CO2 in the process of growth and reproduction by preventing the conversion of formic acid into CO2.Results:The (bio)chemical conversion of formic acid to CO2 is a key reaction. Therefore, we compared the growth of BL21, being a subfamily of K12, alongside two strains in which two different genes related to the formate metabolism were deleted, in complex and simple media. Experimental results were entirely consistent with metabolic predictions. Subsequently, the knockout bacteria grew more efficiently than BL21. Interestingly, TsFDH, a formate dehydrogenase with the tendency of converting CO2 to formate, increased the growth of all strains compared with cells without the TsFDH. Most mutants grew in a simple medium containing glycerol, which showed that glycerol is the preferred carbon source compared to glucose for the growth of E. coli. Conclusion:These results explain the reasons for the inconsistency of predictions in previous metabolic models that declared glycerol as a suitable carbon source for the growth of E. coli but failed to achieve it in practice. To conduct a more mechanistic evaluation of our observations, RNA sequencing data analysis was conducted on an E. coli RNA-seq dataset. The gene expression correlation outcome revealed the increased expression levels of several genes related to protein biosynthesis and glycerol degradation as a possible explanation of our observations.
SP/NK-1R Axis Promotes Perineural Invasion of Pancreatic Cancer and is Affected by lncRNA LOC389641
Authors: Ji Tengfei , Ma Keqiang , Wu Hongsheng , Cao# Tiansheng 
Published: December 2021

Abstract
Background

Pancreatic cancer is a deadly disease with low overall survival during the past 30 years. Perineural invasion (PNI) was considered to be the main reason for poor prognosis. In the present study, we analyzed the role of substance P (SP)/neurokinin-1 receptor (NK-1R) and long non-coding RNA (lncRNA) LOC389641 on pancreatic cancer PNI.
Material and methods

Pancreatic cancer cell lines of BxPC-3 and MIAPaCa-2 were co-cultured with SHSY5Y cells, and then stimulated with SP to simulate the in vivo influence of pancreatic cancer by ganglion. The co-culture cells were transfected with overexpressed neurokinin-1 receptor (NK-1R), silenced NK-1R, overexpressed LOC389641, and silenced LOC389641. Enzyme-linked immunosorbent assay (ELISA) assay was performed to examine the concentration of SP in cells. The cell proliferation ability was assessed by cell counting kit-8 and 5-ethynyl-2’-deoxyuridine (EdU) assays. The wound-healing and Transwell assays were carried out to determine the cell migration and invasion analyses. Quantitative real-time PCR (qRT-PCR), western blot, immunofluorescence (IF) analyses were performed to evaluate the expression levels.
Results

Addition of SP in co-culture system positively regulates cell proliferation, migration, and invasion of pancreatic cancer PNI. SP significantly stimulated NK-1R/Akt/NF-κB signaling pathway. The concentration of 100 nmol/L for 24h was chosen to be optimal for SP treatment.NK-1R positively regulated the proliferation, migration, and invasion of pancreatic cancer PNI. The expression of lncRNA LOC389641 and mRNA tumor necrosis factor receptor SF10A (TNFRSF10A) was not affected by SP. Overexpressed and silenced LOC389641 can correspondingly change the effect of SP stimulation on pancreatic cancer PNI.
Conclusion

We found that SP/NK-1R and LOC389641 promote the cell progression of pancreatic cancer PNI. Moreover, we assumed that the pancreatic cancer PNI promoted by SP/NK-1R axis may be blocked by the TNFRSF10A/NF-κB pathway mediated by LOC389641.
Preclinical Trial of the Effectiveness of the Safety Nasogastric Tube to Detecting Tube Position Based on Tidal Volume and Pepsin Assay Results in the Gastrointestinal Tract of Macaca Fascicularis
Authors: Kartinah Neng Tine , Nuzul Sigit Mohammad , Nur Busjra M. , Ibrahim Ermita Isfandiary 
Published: December 2021

Abstract
Background: Generally, insertion of a nasogastric tube (NGT) does not use imaging guidance. This procedure has a risk of malposition to the lungs from 0.3–15%. The NGT verification only detects the position of the tube in the end of procedure. Misplacement of NGT into the respiratory tract can result in damage to the lungs. Safety nasogastric tube (SNGT) has been created to detect the position of the tube in real-time, simple, and inexpensive. This study aims to prove the effectiveness of the SNGT prototype in Macaca fascicularis. Result: The SNGT with an airbag size of 50% of tidal volume (SNGT 50% TV) had 100% sensitivity and specificity in detecting the position of the tube. While the SNGT with an airbag size of 100% of TV (SNGT 100% TV) has sensitivity of 100% and specificity of 87.5%. There was significant difference between the movement of airbag of SNGT 50% TV and SNGT 100% TV (p ≤ 0.05). However, there was no significant difference between the accuracy of placement of 50% TV SNGT, 100% TV SNGT, and conventional NGT (p > 0.05). The pepsin enzyme has better sensitivity (100%) than pH paper (91.66%) in detecting the end position of tube. Conclusion: SNGT tube has high effectiveness in detecting the position of the tube inside of the respiratory and digestive tracts to prevent misplacement.
Prediction Models for Radiation-Induced Neurocognitive Decline in Adult Patients with Primary or Secondary Brain Tumors: A Systematic Review
Authors: Tohidinezhad Fariba , Perri Dario Di , Zegers Catharina M.L. , Dijkstra Jeanette , Anten Monique , Dekker Andre , Elmpt Wouter Van , Eekers Daniëlle BP , Traverso Alberto 
Published: December 2021

Abstract
Purpose: Although an increasing body of literature suggests a relationship between brain irradiation and deterioration of neurocognitive function, it remains as the standard therapeutic and prophylactic modality in patients with brain tumors. This review was aimed to abstract and evaluate the prediction models for radiation-induced neurocognitive decline in patients with primary or secondary brain tumors.Methods: MEDLINE was searched on October 31, 2021 for publications containing relevant truncation and MeSH terms related to “radiotherapy”, “brain”, “prediction model”, and “neurocognitive impairments”. Risk of bias was assessed using the Prediction model Risk Of Bias ASsessment Tool.Results: Of 3,580 studies reviewed, 23 prediction models were identified. Age, tumor location, education level, baseline neurocognitive score, and radiation dose to the hippocampus were the most common predictors in the models. The Hopkins verbal learning (n=7) and the trail making tests (n=4) were the most frequent outcome assessment tools. All studies used regression (n=14 linear, n=8 logistic, and n=4 Cox) as machine learning method. All models were judged to have a high risk of bias mainly due to issues in the analysis.Conclusion: Existing models have limited quality and are at high risk of bias. Following recommendations are outlined in this review to improve future models: develop a standardized instrument for neurocognitive assessment in patients with brain tumors; adherence to model development and validation guidelines; careful choice of candidate predictors according to the literature and domain expert consensus; and considering radiation dose to brain substructures as they can provide important information on specific neurocognitive impairments.
The Effects Of Some Insecticides On Honeybees (Apis mellifera)
Authors: Akça Rıfat , Saruhan Islam 
Published: December 2021

Abstract
In the study, the topical application (1 µl/bee), contact (5 ml/pot/10 bees) and residual (5 ml/pot/10 bees) effects of eight insecticides (Methiocarb, Alphacypermethrin, Indoxacarb, Spinosad, Thiacloprid + Deltamethrin, Thiamethoxam, Thiamethoxam + Lambda-Cyhalothrin and Zeta Cypermethrin), which are commonly used in pest control in hazelnut cultivation, were investigated on Apis mellifera L. (Hymenoptera:Apidae). The study was conducted with 10 young worker bees in 4 replicates. Knock-down effect and 48-hour mortality were determined in all three methods. The study was conducted under 65-70% humidity and 24 ± 1 oC laboratory conditions. The study findings demonstrated that the impact of the topical application was low in all pesticides, while the other two methods led to 100% mortality after 48 hours at the recommended dose. The highest contact effect was observed with thiamethoxam + lambda-cyhalothrin, thiamethoxam, zeta cypermethrin, methiocarb and indoxacarb, followed by thiacloprid + deltamethrin, Spinosad and alphacypermethrin. Among the insecticides tested for residual effects, thiamethoxam + lambda-cyhalothrin, thiamethoxam, zeta cypermethrin and Spinosad led to over 90% mortality after 5 days.
Real World Data on Cardiometabolic Diseases in U.S. Adults During the SARS-CoV-2 Pandemic: A Decentralized Registry Study
Authors: Shah Parth , Magee Kim , Buccellato Kiara H , Ismond McKenna , Watson Jalisa 
Published: December 2021

Abstract
Background: Pre-existing cardiometabolic comorbidities place SARS-CoV-2 positive patients at a greater risk for poorer clinical course and mortality than those without it. We aimed to analyze real-world registry data focused primarily on participants with cardiometabolic diseases (CMD), which were remotely obtained via a digital platform. Methods: Participants were divided into two groups: CMD or no cardiometabolic disease (non-CMD). They were evaluated based on their medical history, current medications/supplements, COVID-19 status, demographics, and baseline characteristics. The frequency of medications/supplements for CMD were compared using relative risks and 95% confidence intervals. Results: The 791 enrollees represented 49 U.S. states. The CMD group had significantly higher (p<0.0001) BMI (mean >30kg/m2) and age (mean >9 years) compared to non-CMD group. In the CMD group, participants who tested positive for COVID-19 had lower (p<0.0001) well-being scores than those without COVID-19. For the 274 participants on CMD medications/supplements, there was no statistical difference in risk of COVID-19 contracture based on medication/supplement type; however, all six participants who were not being treated for CMD were COVID-19 positive (RR ~104). For 89 participants who were on treatment for diabetes or insulin resistance, there was a 90% reduced risk of COVID-19 incidence (p = 0.0187). Conclusion: The well-being score of the CMD group was dependent on whether they tested positive for COVID-19. Type of CMD treatment did not impact COVID-19 status, but absence of treatment significantly increased COVID-19 incidence. With respect to SARS-CoV-2, our analysis supports continued use of the statins, ACE-I, ARBs, and diabetes medications in CMD patients.ClinicalTrials.gov Identifier: NCT04348942
Ego-resiliency Moderates the Risk of Depression and Social Anxiety Symptoms on Suicidal Ideation in Medical Students
Authors: Seo Eun Hyun , Yang Hae-Jung , Kim Seung-Gon , Yoon Hyung-Jun 
Published: December 2021

Abstract
BackgroundLittle is known about the role of protective factors in suicidal ideation among medical students. This study aimed to examine the association between suicidal ideation and protective (self-esteem/ego-resiliency/social support) and risk (depression/social anxiety) factors.MethodsData on sociodemographic factors, depression, social anxiety, self-esteem, ego-resiliency, social support, and current suicidal ideation were collected from 408 medical students. A logistic regression model was constructed to identify the independent impact of potential influencing factors on suicidal ideation. Potential moderating effects were also explored.ResultsThirty-eight participants (9.3%) reported experiencing suicidal ideation. Younger age, higher levels of depression, social anxiety, and lower levels of self-esteem, ego-resiliency, and social support were found to be significantly correlated with suicidal ideation. In the final model, higher levels of depression and social anxiety were associated with an increased risk of suicidal ideation, while higher levels of self-esteem and social support were associated with a decreased risk of suicidal ideation. Although the independent effect was not significant, the interactions of ego-resiliency with both depression and social anxiety on suicidal ideation were significant. Higher levels of ego-resiliency acted as a buffer against suicidal ideation among those with higher levels of depression or social anxiety.ConclusionsIn addition to risk factors, this study revealed the underlying protective and moderating factors of suicidal ideation among medical students. Mental health programs focusing on enhancing ego-resiliency, self-esteem, and social support may contribute to suicide prevention in medical students.
Antimicrobial Activities of Fungus Comb Extracts Isolated From Indo-Malayan Termite Macrotermes Gilvus Mound
Authors: Witasari Lucia Dhiantika , Wahyu Khairunnasa Wizdjanul , Anugrahani Bonifasia Junita , Kurniawan Dina Clarissa , Haryanto Aris , Nandika Dodi , Karlinasari Lina , Arinana Arinana , Batubara Irmanida , Santoso Djoko , Rachmayanti Yanti , Firmansyah Dikhi , Sudiana I Ketut , Hertanto Decsa Medika 
Published: December 2021

Abstract
The embodiment of antimicrobial components into the food packaging material has the ability to prevent microbial contamination. Fungus comb could be an alternative source of natural antimicrobial agents. In this study, n-hexane, ethyl acetate, methanol, and water extracts from fungus comb isolated from Indo-malayan termite Macrotermes gilvus Hagen mound were analysed for its antibacterial and antifungal activities against food spoilage microorganisms, including Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, Staphylococcus aureus ATCC 25923, Aspergillus flavus, and Aspergillus niger. The antimicrobial activity of the fungus comb extracts was evaluated using Kirby-Bauer disc diffusion and microdilution method. The result showed that ethyl acetate extract exhibited the biggest diameter inhibition zone for all bacteria and fungi tested. Ethyl acetate extract showed antibacterial activity in all bacteria with minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values of 0.39 mg/mL and 0.78 mg/mL, respectively. This extract also inhibited A. flavus and A. niger with MIC value of 0.78 mg/ml. Ethyl acetate extract contained guaiacol and syringol, which were predicted as the main antimicrobial components in fungus comb. Whereas n-hexane extract only inhibited Gram-positive bacteria. S. aureus ATCC 25923 was the most sensitive bacteria tested using all extracts. In addition, A. flavus was more sensitive compared to A. niger. Overall, fungus comb extract exhibited antimicrobial activity against E.coli ATCC 25922, P. aeruginosa ATCC 27853, S. aureus ATCC 25923, A. flavus, and A. niger. This study revealed that the fungus comb extract, especially ethyl acetate, could be considered as a new antimicrobial agent.
The characteristics of weather-related wind load parameters and wind 2 power quality over the Dabanchen strong gale area
Authors: Xin Yu , Liu Zonhui , He Qing 
Published: December 2021

Abstract
Analysis of data from cup and ultrasonic anemometers on a 100 m-tall wind mast in the Dabanchen Canyon reveals that the turbulence intensities, gust factors, and peak factors measured by cup anemometers tend to be severely undervalued compared to longitudinal values from an ultrasonic anemometer, and onsite three-dimensional measurement data are preferred for weather-related wind load calculation. This difference is related to the rotating responses of cup anemometers during wind speed acceleration or deceleration and the higher vertical fluctuation speeds driven by dynamic interactions with the sloped canyon terrain. The higher lateral turbulence is key consideration for determining wind turbine classes in Danbanchen strong wind area. The longitudinal gust and peak factors under wind speeds exceeding 25.0 m s−1 are comparable with those of typhoon-prone open, flat regions. The chances of high turbulence and unstable stratification convective processes are very high; thus, wind power collection efficiencies are not high. Except the longitudinal turbulence integral scale, China’s wind-resistance codes are not applicable due to occasional undesirable strong gales with extraordinary turbulence structures. The measured vertical power spectral density of fluctuating wind in the high-frequency domain cannot reflect the rapidly adapting features of the vast terrain under strong gales.
Indicators of Infertility and Fertility Care: A Systematic Scoping Review
Authors: Nabhan Ashraf , Kamel Menna , Abuelnaga Yasmeen , Ghonim Mohanad , Elshafeey Farida , Magdi Rana , Gebril Sarah , Mahdy Shahd , Sarhan Dana , Mburu Gitau , Kiarie James 
Published: December 2021

Abstract
Background: The identification of appropriate, relevant and valid indicators of infertility and fertility care is critical to effective monitoring of progress in fertility care access, equity, utilization and impact globally. The aim of the review was to map existing literature on indicators of infertility and fertility care and summarize these indicators by typology and dimensions.Methods: This review followed the framework of Arksey and O’Malley. Bibliographic databases of published studies (MEDLINE, PubMed, JSTOR, CINAHL, Web of Science and Scopus) were searched from inception to June 2021. We also searched unpublished datasets and hand-searched reference lists of relevant articles. Two reviewers independently screened reports and extracted data onto a data charting table before collating and summarizing results.Results: We included 46 reports from 88 countries. The reporting of data was either voluntary in 63 countries (72%) and compulsory in 25 countries (28%). The reporting of data was partial in 63 countries (72%) and complete in 25 countries (28%). Reporting for cycles or deliveries were based on individual cycles in 56 countries (64%) and on cumulative cycles in 32 countries (36%). In types of overall typology, most of the indicators were outcome indicators (70%) with fewer being process indicators (16%) or structural indicators (14%). In terms of dimension, of most indicators were effectiveness and efficiency indicators. Indicators on safety, patient-centeredness, equity, and timeliness were under-reported.Conclusion: A wide range of indicators of infertility and fertility care exist in literature; however, most are outcomes indicators of effectiveness and efficiency dimensions, while those on safety, patient-centeredness, equity, and timeliness and non-clinical aspects are limited. These findings provide a basis for selection and prioritization of a core set of indicators to monitor progress in fertility care.Systematic review registration: Open Science Framework (osf.io/78wrg)
Changes in Spike Protein Antibody Titer Over 90 Days after the Second Dose of SARS- CoV-2 Vaccine in Japanese Dialysis Patients
Authors: Wakai Haruki , Abe Natsumi , Tokuda Touno , Yamanaka Rika , Ebihara Satoshi , Izumaru Kensuke , Ishii Daisuke , Hyodo Toru , Yoshida Kazunari 
Published: December 2021

Abstract
Objectives There is no report on antibody titers after vaccination against SARS-CoV-2 in Japanese dialysis patients. As dialysis is different between Japan and other countries, changes in antibody titers were examined. Methods Baseline characteristics and anti-spike protein antibody titers (Roche) over 90 days after administration of the BNT162b2 messenger RNA vaccine were investigated in dialysis patients. Results The maximum anti-spike protein antibody titer after the second dose was 737.9 (326.8 to 1143.4) and was reached at 19 (17 to 24.3) days after the second dose. Antibody titers decreased over time, with titers of 770 (316.4 to 1089) at 15 days, 385 (203 to 689.7) at 30 days, 253.5 (138 to 423) at 60 days, and 208 (107 to 375) at 90 days after the second dose. When an antibody titer of 137.052 U/mL was assumed to be a measure related to breakthrough infection, the proportion of subjects with antibody titers exceeding this level was 90.1％ at 15 days, 85.3% at 30 days, 75.0% at 60 days, and 65.4% at 90 days after the second dose. When a decrease in antibody titers below the assumed breakthrough level was defined as an event, subjects with a pre-dialysis albumin ≥ 3.5 were significantly less likely to experience an event than subjects with a pre-dialysis albumin < 3.5. In addition, this study suggests that the presence of anti-spike protein levels ≥ 313 at 30 days after the second vaccine dose might be a factor in maintaining antibody titers exceeding the assumed breakthrough level at 90 days after the second dose. Conclusions The maximum antibody titers and the antibody titers at 90 days after the second vaccine dose in Japanese dialysis patients were approximately 30% of those in non-dialysis individuals reported otherwise, it was similar to those in dialysis patients in other countries. Whether an additional vaccine dose is needed should be determined based on indicators serving as factors in maintaining antibody titers as well as the status of the spread of infection.
The genetic diversity of Asplenium viride (Aspleniaceae) fern colonizing heavy metal-polluted sites
Authors: Woch Marcin , Grzegorz  , Jedrzejczyk Iwona , Podsiedlik Marek , Stefanowicz Anna 
Published: December 2021

Abstract
Heavy metals can affect the morphology, physiology and evolution of plants. Asplenium viride is a diploid species, belonging to the largest genus of the cosmopolitan fern family Aspleniaceae, and occurring on various types of alkaline rocks. It is known to colonize sites with high concentrations of heavy metals, exhibiting changes in frond morphology. Microevolutionary processes, manifesting as changes in genome size and new genotype formation, can ultimately lead to the formation of new subspecies and speciation. This study aimed to evaluate the morphological and genetic diversity of A. viride, and to test for a potential correlation between variability and heavy metal concentration. Analysis of A. viride specimens, from one metalliferous site and five non-metalliferous localities, showed no apparent variation in genome size between plants from affected and non-affected sites. There was no significant correlation between genetic variability and heavy metal concentration. This was possibly due to intragametophytic selfing, caused by patchy habitats and subsequent founder effects, resulting from long-distance colonization by single spores.