bluuebunny/crossref_metadata_2025_split huggingface dataset:
Social isolation is linked to declining grey matter structure and cognitive functions in the LIFE-Adult panel study
Authors: Lammer Laurenz , Beyer Frauke , Luppa Melanie , Sander Christian , Baber Ronny , Engel Christoph , Wirkner Kerstin , Loeffler Markus , Riedel-Heller Steffi G , Villringer Arno , Witte Veronica 
Published: December 2021

AbstractSocial isolation has been suggested to increase the risk to develop cognitive decline. However, our knowledge on causality and neurobiological underpinnings is still limited. In this preregistered analysis, we tested the impact of social isolation on central features of brain and cognitive aging using a longitudinal population-based magnetic resonance imaging (MRI) study. Assaying 1335 cognitively healthy participants (50-80 years old, 659 women) at baseline and 895 participants after ∼6 years follow-up, we found baseline social isolation and change in social isolation to be associated with smaller volumes of the hippocampus, reduced cortical thickness and poorer cognitive functions. Combining advanced neuroimaging outcomes with prevalent lifestyle characteristics from a well-characterized population of middle- to older aged adults, we provide evidence that social isolation contributes to human brain atrophy and cognitive decline. Within-subject effects of social isolation were similar to between-subject effects, indicating an opportunity to reduce dementia risk by promoting social networks.
Epigenetic remodeling during monolayer cell expansion reduces therapeutic potential
Authors: Scott Adrienne K. , Casas Eduard , Schneider Stephanie E. , Swearingen Alison R. , Van Den Elzen Courtney L. , Seelbinder Benjamin , Barthold Jeanne E. , Kugel Jennifer F. , Stern Josh Lewis , Foster Kyla J. , Emery Nancy C. , Brumbaugh Justin , Neu Corey P. 
Published: December 2021

ABSTRACTUnderstanding how cells remember previous mechanical environments to influence their fate, or mechanical memory, informs the design of biomaterials and therapies in medicine. Current regeneration therapies require two-dimensional (2D) cell expansion processes to achieve large cell populations critical for the repair of damaged (e.g. connective and musculoskeletal) tissues. However, the influence of mechanical memory on cell fate following expansion is unknown, and mechanisms defining how physical environments influence the therapeutic potential of cells remain poorly understood. Here, we show that the organization of histone H3 trimethylated at lysine 9 (H3K9me3) and expression of tissue-identifying genes in primary cartilage cells (chondrocytes) transferred to three-dimensional (3D) hydrogels depends on the number of previous population doublings on tissue culture plastic during 2D cell expansion. Decreased levels of H3K9me3 occupying promoters of dedifferentiation genes after the 2D culture were also retained in 3D culture. Suppression of H3K9me3 during expansion of cells isolated from a murine model similarly resulted in the loss of the chondrocyte phenotype and global remodeling of nuclear architecture. In contrast, increasing levels of H3K9me3 through inhibiting H3K9 demethylases partially rescued the chondrogenic nuclear architecture and gene expression, which has important implications for tissue repair therapies, where expansion of large numbers of phenotypically-suitable cells is required. Overall, our findings indicate mechanical memory in primary cells is encoded in the chromatin architecture, which impacts cell fate and the phenotype of expanded cells.SIGNIFICANCE STATEMENTTissue regeneration procedures, such as cartilage defect repair (e.g. Matrix-induced Autologous Chondrocyte Implantation) often require cell expansion processes to achieve sufficient cells to transplant into an in vivo environment. However, the chondrocyte cell expansion on 2D stiff substrates induces epigenetic changes that persist even when the chondrocytes are transferred to a different (e.g. 3D) or in vivo environment. Treatments to alter epigenetic gene regulation may be a viable strategy to improve existing cartilage defect repair procedures and other tissue engineering procedures that involve cell expansion.
Pharmacokinetics and Pharmacodynamics of Vancomycin derivative LYSC98 in a Murine Thigh Infection Model against Staphylococcus aureus
Authors: He Peng , Li Xin , Guo Xiaohan , Bian Xingchen , Feng Meiqing 
Published: December 2021

LYSC98 is a vancomycin derivative used for gram-positive bacterial infections therapy. We reported the pharmacokinetic/pharmacodynamic (PK/PD) targets of LYSC98 against Staphylococcus aureus using a murine thigh infection model. Three Staphylococcus aureus strains were utilized. Single-dose plasma pharmacokinetics of LYSC98 were determined in infected mice after the tail vein injection of 2, 4, and 8mg/kg. The results showed maximum plasma concentration (Cmax) 11466.67 - 48866.67 ng/mL, area under the concentration-time curve from 0 to 24 h(AUC0-24) 14788.42 -91885.93 ng/mL·h, and elimination half-life(T1/2) 1.70-2.64 h, respectively. The Cmax (R2 0.9994) and AUC0-24 (R2 0.981) were positively correlated with the dose of LYSC98 in the range of 2-8 mg/kg.Dose fractionation studies using total doses of 2 to 8 mg/kg administered with q6h, q8h, q12h, and q24h were performed to evaluate the correlation of different PK/PD indices with efficacy. Sigmoid model analysis showed Cmax/MIC (R2 0.8941) was the best PK/PD index to predict the efficacy of LYSC98. In the dose ranging studies, two Methicillin-resistant Staphylococcus aureus (MRSA) clinical strains were used to infect the mice and 2-fold-increasing doses (1 to 16 mg/kg) of LYSC98 were administered. The magnitude of LYSC98 Cmax/MIC associated with net stasis, 1, 2, 3 and 4 - log10 kill were 5.78, 8.17, 11.14, 15.85 and 30.58, respectively. The results of this study showed LYSC98 a promising antibiotic with in vivo potency against MRSA, and will help in the dose design of phase one study for LYSC98.
Change in the trend of long-term care service usage following COVID-19 pandemic in Japan: a survey using nationwide statistical summary in 2018-2021
Authors: Sato Kenichiro , Niimi Yoshiki , Iwatsubo Takeshi , Ishii Shinya 
Published: December 2021

AbstractAimSocial restriction due to coronavirus disease 2019 (COVID-19) pandemic forced long-term care (LTC) service users to refrain from using services as before, of which degree of change we aim to evaluate in this study.MethodsWe retrospectively analyzed publicly-distributed nationwide statistics summarizing the monthly number of public LTC insurance users in Japan in the period between April 2018 and March 2021. The degree of decline was quantified as odds ratio (OR), where the ratio of a certain month to the reference month was divided by the ratio in the previous year.ResultsThe use of LTC services showed unimodal serial change: it started to decline in March 2020 and reached its largest decline in May 2020, which had insufficiently recovered even as of late 2020. The degree of decline was specifically large in services provided in facilities for community-dwelling elderly individuals (adjusted OR 0.719 (95%CI: 0.664 ∼ 0.777) in short-stay services and adjusted OR 0.876 (95%CI: 0.820 ∼ 0.935) in outpatient services) but was non-significant in other types of service, including those provided for elderly individuals living in nursing homes.ConclusionsCurrent study showed that community-dwelling elderly individuals who had used outpatient or short-stay services were the segments which were specifically affected by the COVID-19 pandemic in 2020 Japan. It underlines the need for further investigation for the medium- or long-term influence on the mental and physical health of these LTC service users as well as their family caregivers.
RB1 loss triggers dependence on ESRRG in retinoblastoma
Authors: Field Matthew G. , Kuznetsoff Jeffim N. , Zhang Michelle G. , Dollar James J. , Durante Michael A. , Sayegh Yoseph , Decatur Christina L. , Kurtenbach Stefan , Pelaez Daniel , Harbour J. William 
Published: December 2021

AbstractRetinoblastoma (Rb) is a deadly childhood eye cancer that is classically initiated by inactivation of the RB1 tumor suppressor. Clinical management continues to rely on nonspecific chemotherapeutic agents that are associated with treatment resistance and toxicity. Here, we analyzed 103 whole exomes, 16 whole transcriptomes, 5 single-cell transcriptomes, and 4 whole genomes from primary Rb tumors to identify novel Rb dependencies. Several recurrent genomic aberrations implicate estrogen-related receptor gamma (ESRRG) in Rb pathogenesis. RB1 directly interacts with and inhibits ESRRG, and RB1 loss uncouples ESRRG from negative regulation. ESRRG regulates genes involved in retinogenesis and oxygen metabolism in Rb cells. ESRRG is preferentially expressed in hypoxic Rb cells in vivo. Depletion or inhibition of ESRRG causes marked Rb cell death which is exacerbated in hypoxia. These findings reveal a novel dependency of Rb cells on ESRRG, and they implicate ESRRG as a potential therapeutic vulnerability in Rb.
Building information modelling and building sustainability assessment: a review
Authors: Nairne Schamne Annelise , Nagalli André , Soeiro Alfredo Augusto Vieira 
Published: December 2021

PurposeThe construction sector is increasingly engaged to explore the potential application of building information modelling (BIM) for the sustainability of the buildings. BIM can be applied conjugated to certification methods to evaluate the sustainability of the buildings, and it is known as building sustainability assessment (BSA). This article aims to explore applications of BIM in the sustainability analysis of buildings, including five types of BSA.Design/methodology/approachA literature review from 2009 to 2020 considering a combination of search terms related to BIM and BSA complemented the analysis. The bibliometric analysis indicates an increase in the number of publications on this topic of study in the last five years. A peak occurred in 2015 and 2020, with 51 and 66 publications, respectively.FindingsThe results showed that BIM contributes to achieving part of the sustainability requirements from BSA, especially quantitative requirements that are related to material and resources. However, a complete analysis between BIM and BSA is still a challenge since part of BSA's have a lack information in their requirements and BIM softwares present interoperability problems that difficult an integrated analysis to extract information directly from the software to attend BSA's requirements.Originality/valueThe results contribute to analysing the evolution of studies about BIM and to understand the limitation in the applicability with sustainability rating systems. Future directions of research indicate the necessity to improve the use of BIM in the sustainability analysis of the buildings, but with a good perspective regarding the application of BIM in material and resources requirements, and waste management in construction sites.
Towards identifying knowledge bases in KIBS through their service development process
Authors: Krupskaya Anastasia , Pina Katia Oliveira 
Published: December 2021

PurposeThe purpose of this paper is to explore knowledge-intensive business services (KIBS), to understand how they implement their service development (SD) processes and how these processes are related to their knowledge bases (KB) as not enough studies have focussed on the understanding of KIBS heterogeneity, by analysing the nature and essence of these firms’ activities.Design/methodology/approachThe work adopts the case study approach involving 12 KIBS firms with different KB according to Bjorn Asheim and colleagues’ “synthetic, analytical, symbolic model”, which identifies synthetic, analytical and symbolic primary KB.FindingsThe research results showed how the SD processes are systemised in KIBS companies depending on the KB. The proposed empirical method for defining KB confirmed the assumed existence of a new type or subtype of KB for legal companies “Compliance/Interpretive”.Research limitations/implicationsFurther research, based on a larger number of companies, is needed to confirm or complement the results of this research, as well as to find more methods to define and evaluate KB on an empirical basis.Practical implicationsThe correct classification of the applied KB can help a company to create effective processes for developing innovative services, while understanding of knowledge dynamic in general – to organise a working process that corresponds to employees’ needs and is more adaptable to the changes in society.Originality/valueThe study proposed a new empirical method for defining and evaluating KB in KIBS companies based on their SD processes. This study makes a significant contribution to research by identifying knowledge-integration practices needed to enhance innovation performance.
Industry 4.0 competencies: a model for the Vietnamese workforce
Authors: Nguyen Anh Tuan 
Published: December 2021

PurposeThis study aims to develop a competency model for the Vietnamese workforce in the era of Industry 4.0.Design/methodology/approachA combination of an online survey and a Delphi study was conducted to identify a set of skills, abilities, knowledge and personalities that are critical for effective performance in the future workplace.FindingsA set of 26 competencies was identified. Similar to previous findings in the context of developed countries, the set centres around a group of competencies commonly called “21st century competencies”. It also includes characteristics unique to a developing nation.Research limitations/implicationsThe proposed framework of competencies may not cover all human capabilities necessitated by the workplace and may include items with overlapping definitions. The study only represents the view of the business community, and its survey sample was limited in size and location. The impact of COVID-19 on the competency model was not explicitly covered during the study.Practical implicationsThe competency model could serve as a basis for the educator to reform curricula, the policy maker to devise policies and the employer to develop training programmes. It can be used as a reference for other nations at similarly-developmental level.Originality/valueThe present study is among a few empirical studies regarding a model for Industry 4.0 competencies in the context of low middle-income countries and probably provides one of the first Industry 4.0 competency models for Vietnam. It also provides a novel framework for identifying and analyzing competencies.
Diversification or specialisation? Farmers' cropping strategy and economic performance under climate change in China
Authors: Wu Mingze , Zhu Yueji , Yang Qi 
Published: December 2021

Purpose
Farmers' adaptation strategies in agricultural production are required to minimise the negative impact of climate change on a nation's food production in developing countries. Based on the panel data of the provincial level in China from 2000 to 2017, this study aims to analyse the changing climate over recent years and farmers' adaptation strategy in terms of cropping in agricultural production.

Design/methodology/approach
This study uses Simpson's diversity index (SDI) to measure the degree of crop diversity planted by farmers and evaluate the influence of climate change on farmers' cropping strategy using the fixed-effect model. Further, the authors estimate the impact of farmers' cropping strategy on their economic performances in two aspects including yields and technical efficiency of crops.

Findings
The empirical results show that the overall climate appears a warming trend. Different from farmers in some other countries, Chinese farmers tend to adopt a more specialised cropping strategy which can significantly improve the technical efficiency and yields of crops in agriculture. In addition, as a moderating role, the specialised cropping can help farmers to alleviate the negative impact of climate change on technical efficiency of their crops.

Originality/value
First, previous studies showed that the changing climate influenced farmers' adaptation strategies, while most studies focussed on multiple adaptation strategies from the farm-level perspective rather than cropping strategy from the nation-level perspective. Second, the present study investigates how the cropping strategy affects the economic performance (in terms of the technical efficiency and crop yields) of agricultural production. Third, the stochastic frontier analysis method is used to estimate the technical efficiency. Fourth, this study explores the moderating effect between farmers' cropping strategy and technical efficiency by introducing an interaction item of SDI and accumulated temperature.
Fun at workplace and intention to leave: role of work engagement and group cohesion
Authors: Jyoti Jeevan , Dimple Dimple 
Published: December 2021

PurposeThe purpose of this study is to examine the role of work engagement (WE) and group cohesion (GC) in the context of fun at workplace (FAW) and its outcomes.Design/methodology/approachIn total, 753 employees of fast food chain restaurants in India have been contacted, and 244 responded back. Exploratory and confirmatory factor analyses have been conducted to assess the reliability and validity of the data. Further, the hypothesized relationships have been tested using structural equation modeling.FindingsThe findings of the study revealed that WE mediates the relationship between FAW and intention to leave (ITL). Further, GC moderates the relationship between FAW and WE.Research limitations/implicationsThis research has been conducted in fast food chain restaurants only, and the data is cross-sectional in nature.Practical implicationsOrganizations should develop a business culture based on fun and enjoyment, which will help in creating a comfortable environment for employees and reduce their ITL.Social implicationsSociety benefits a lot if employees have positive work experiences. FAW will help to reduce the negative spillover of work pressure/stress to the home. Employees will come home with minimum work stress and will contribute positively to their personal and social life.Originality/valueThe present study empirically tested the mediating role of WE between FAW and ITL and moderating role of GC between FAW and WE that helped a lot to understand the relationships in the workplace setting.
An exploration of changing student entrepreneurial motivators – a longitudinal analysis
Authors: Figueiredo Belchior Ricardo , Lyons Roisin 
Published: December 2021

Purpose Entrepreneurial motivations are considered key determinants of the direction, intensity and duration of entrepreneurial processes and outcomes. To measure attitudes and outcome expectations related to an entrepreneurial career, researchers often use a set of predetermined behavioral beliefs or motivators. However, motivators can be numerous, context-specific and there is a lack of evidence regarding their stability over time. This study addresses this gap, while also providing a rich description of how Portuguese college students perceive entrepreneurship and how these perceptions relate to levels of entrepreneurial intentions (EI).Design/methodology/approach Using qualitative and quantitative methods to analyze data over a 5-year period, this study seeks to confirm existing evidence on entrepreneurship motivators' diversity and heterogeneity and to explore differences in motivators' temporal stability. Using an initial sample of 851 Portuguese college students and 3 different survey waves, this study first aggregates the most frequently cited motivators for EI to compile a series of meaningful motivational factors. Subsequently, it extensively analyzes the robustness of these factors by examining their validity against EI and stability over time.Findings Based on their superior association with EI and greater temporal stability, the findings suggest that entrepreneurship-intrinsic motivational factors are more relevant for college students' EI models. Prominently, being motivated to create one's own new business due to a particular occupational interest is the most consistently stable individual motivation.Practical implications The results may interest those studying entrepreneurial motivations and EI longitudinally and who invest in promoting entrepreneurial behavior of college students.Originality/value To the best of the authors’ knowledge, this is the first study to analyze entrepreneurship motivators/reasons' temporal stability, which is relevant for assessing their value for longitudinal entrepreneurship research and education. It is also the most complete assessment of Portuguese college students' perceptions of entrepreneurship.
Barriers to bank customers’ intention to fully adopt digital payment methods
Authors: Dimitrova Irina , Öhman Peter , Yazdanfar Darush 
Published: December 2021

Purpose
The purpose of this study is to empirically investigate the relationship between a set of functional and social–psychological barriers and bank customers’ intention to fully adopt digital payment methods (DPMs).

Design/methodology/approach
The data were collected via an online questionnaire sent to two samples of Swedish bank customers, namely, adopters-accepters (i.e. young bank customers) and adopters-resisters (i.e. a group opposing a cashless society). Hypotheses were tested by applying an ordinal regression model.

Findings
Regarding the adopters-accepters, privacy and access barriers can be obstacles to the full adoption of DPMs. The adopters-resisters perceived all five studied barriers as significant, though only the impersonalisation barrier seemed to matter when the barriers were related to their intention to fully adopt DPMs. Moreover, the results suggest that barriers have a stronger negative effect on the intention to fully adopt among those with extensive experience of DPMs.

Practical implications
Based on the barriers affecting the intention of particular groups of bank customers to adopt DPMs, banks could implement customised measures to promote the ongoing development of digital financial services.

Originality/value
In this under-researched area, this study provides empirical knowledge of the influence of various barriers on the intention of bank customers characterised as adopters-accepters and adopters-resisters to fully adopt DPMs.
Exploring perceived innovation in building customers’ patronizing behavior in the food delivery service context
Authors: Ahn Jiseon 
Published: December 2021

PurposeIn the food delivery industry, mobile applications have become an important platform for providing services and interacting with customers. Thus, this study aims to examine whether customers’ perceived innovation changes their patronizing behavior intention toward the brand’s application.Design/methodology/approachUsing data from food delivery customers in the USA, this study identifies the relative impact of multidimensional innovation (i.e. service concept innovation, service process innovation and customer experience innovation) on customers’ behavior. Partial least squares-structural equation modeling is used to test the hypotheses.FindingsResults show that customers’ perceived service process and experience innovation increase commitment, which turns into reuse intention of the delivery application. Commitment also increases the impact of customers’ perceived innovation on loyalty. However, in this study, service concept innovation is insufficient to solely create positive behavioral intention.Practical implicationsFindings suggest that a food delivery application that continues to innovate to improve the service process and experience can be an effective marketing tool because they increase bonding with customers. This study recommends that food delivery companies manage their relationship with customers to achieve sustainable growth.Originality/valueDespite the growing attention in the mobile service industry, limited studies have examined the effects of innovations of food delivery application on customers’ behavior. Thus, this study provides useful guidelines that advance mobile commerce research, especially in the food delivery industry.