bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Lived Experience of Already-Lonely Older Adults During COVID-19
Authors: Bundy Henry , Lee Heather M , Sturkey Kim N , Caprio Anthony J 
Published: June 2021

Abstract

Background and Objectives
From the outset of the coronavirus disease 2019 (COVID-19) pandemic, analysts warned that older populations, due to their age, chronic illnesses, and lack of technological facility, would suffer disproportionately from loneliness as they sheltered in place indefinitely. Several studies have recently been published on the impact of COVID-19-related loneliness among older populations, but little has been written about the experiences of already-lonely older individuals; those who had lived with persistent loneliness before the advent of COVID-19. This qualitative study sought to understand how already-lonely older individuals navigated and endured the social isolation of the pandemic.

Research Design and Methods
Twelve semistructured interviews were conducted with individuals aged 65 or older who scored a 6 or above on the 3-item UCLA Loneliness Risk screening tool. Interviews were coded using the constant comparative method. Themes and understandings of loneliness that reoccurred within and across interviews were identified and collected.

Results
Already-isolated older interviewees did not necessarily experience the abject loneliness hypothesized by analysts. Most interviewees used longstanding arrangements, in place to mitigate loneliness and endure social isolation, to manage the social deprivation of COVID-19. As a result, their loneliness did not compound during long bouts of mandated social isolation. To the contrary, loneliness during the pandemic appeared to carry a new valence for interviewees, as COVID-19 imbued their isolation with new meaning, rendering their loneliness necessary and responsible.

Discussion and Implications
Exploring individuals’ subjective perceptions of loneliness can help provide a deeper understanding of what it means to be isolated and alone during COVID-19 and aid in designing strategies to mitigate loneliness.
Spatial, temporal and cell-type-specific expression profiles of genes encoding heparan sulfate biosynthesis enzymes and proteoglycan core proteins
Authors: Moon Sohyun , Zhao Ying-Tao 
Published: June 2021

Abstract
Heparan sulfate (HS) is a linear polysaccharide found in almost all animal cells and plays an important role in various biological processes. HS functions mainly via covalently binding to core proteins to form HS proteoglycans (HSPGs), which are heterogeneous in the lengths of the HS chain, the modifications on HS and the core proteins. The molecular mechanisms underlying HSPG heterogeneity, although widely studied, are not yet fully defined. The expression profiles of HS biosynthesis enzymes and HSPG core proteins likely contribute to the HSPG heterogeneity, but these expression profiles remain poorly characterized. To investigate the expression profiles of genes encoding HS biosynthesis enzymes and HSPG core proteins, we systematically integrated the publicly available RNA sequencing data in mice. To reveal the spatial expression of these genes, we analyzed their expression in 21 mouse tissues. To reveal the temporal expression of these genes, we analyzed their expression at 17 time points during the mouse forebrain development. To determine the cell-type-specific expression of these genes, we obtained their expression profiles in 23 cell types in the mouse cerebral cortex by integrating single nucleus RNA sequencing data. Our findings demonstrate the spatial, temporal and cell-type-specific expression of genes encoding HS biosynthesis enzymes and HSPG core proteins and represent a valuable resource to the HS research community.
The 2019 Morocco Model BIT: Moving Forwards, Backwards or Roundabout in Circles?
Authors: Banerjee Arpan , Weber Simon 
Published: June 2021

Abstract
The Moroccan Model Bilateral Investment Treaty (BIT) (2019) adds to a growing body of ‘new-generation’ model BITs that have fuelled the conversation on reforming the investment arbitration system. Broadly speaking, such agreements have attempted to rethink means of dispute resolution and traditional investment protection standards while adding detailed investor obligations and standards of conduct. In that context, this article discusses the provisions of the Moroccan Model BIT (2019), highlighting not only progressive developments but also areas of concern. Upon comparing the provisions of the BIT with those of other ‘new-generation’ investment agreements, as well as existing treaty practice, the authors offer the following findings.
Reflection on modern methods: statistical, policy and ethical implications of using age-standardized health indicators to quantify inequities
Authors: Thurber Katherine A , Thandrayen Joanne , Maddox Raglan , Barrett Eden M , Walker Jennie , Priest Naomi , Korda Rosemary J , Banks Emily , Williams David R , Lovett Raymond 
Published: June 2021

Abstract
Methods for calculating health indicators profoundly influence understanding of and action on population health and inequities. Age-standardization can be useful and is commonly applied to account for differences in age structures when comparing health indicators across groups. Age-standardized rates have well-acknowledged limitations, including that they are relative indices for comparison, and not accurate measures of actual rates where the age structures of groups diverge. This paper explores these limitations, and demonstrates alternative approaches through a case study quantifying mortality rates within the Aboriginal and Torres Strait Islander (Indigenous) population of Australia and inequities compared with the non-Indigenous population, over 2001–16. Applying the Australian Standard Population, the Aboriginal and Torres Strait Islander age-standardized mortality rate was more than double the crude mortality rate in 2001 and 2016, inflated through high weighting of older age groups. Despite divergent population age structures, age-standardized mortality rates remain a key policy metric for measuring progress in reducing Indigenous-non-Indigenous inequities in Australia. Focusing on outcomes age-standardized to the total population can obscure inequities, and denies Aboriginal and Torres Strait Islander peoples and communities valid, actionable information about their health and well-being. Age-specific statistics convey the true magnitude of health risks and highlight high-risk subgroups. When requiring standardization, standardizing to a population-specific standard (here, an Indigenous standard) generates metrics centred around and reflective of reality for the population of focus, supporting communities’ self-determination to identify priorities and informing resource allocation and service delivery. The principles outlined here apply across populations, including Indigenous and other populations internationally.
Type 1 Innate Lymphoid Cell and Natural Killer Cells Are Sources of Interferon-γ and Other Inflammatory Cytokines Associated With Distinct Clinical Presentation in Early Dengue Infection
Authors: Quintino-de-Carvalho Iracema Luisa , Gonçalves-Pereira Marcela Helena , Faria Ramos Michele , de Aguiar Milhim Bruno Henrique Gonçalves , Da Costa Último Libânio , Santos Érika Gonçalves , Nogueira Maurício Lacerda , Da Costa Santiago Helton 
Published: June 2021

Abstract

Background
Increased levels of inflammatory cytokines are associated with severe dengue evolution, but the source of such hypercytokinemia is elusive. We investigated the contribution of innate lymphocytes, innate lymphoid cells (ILCs), and natural killer (NK) cells in cytokine production in early dengue infection.

Methods
Peripheral blood mononuclear cells of individuals with dengue without warning signs (DWS–) and dengue with warning signs and severe dengue (SD) presentation combined (DWS+) were obtained between 2 and 7 days since fever onset and submitted to flow cytometry without specific antigen stimulation to evaluate cytokines in ILC and NK cell subpopulations.

Results
ILCs and NK cells were found to be important sources of cytokines during dengue. ILCs of the DWS+/SD group displayed higher production of interferon gamma (IFN-γ) and interleukin (IL) 4/IL-13 when compared to DWS– individuals. On the other hand, NK Eomes+ cells of DWS– patients displayed higher IFN-γ production levels compared with the DWS+/SD group. Interestingly, when NK cells were identified by CD56 expression, DWS+/SD displayed higher frequency of IL-17 production compared with the DWS– group.

Conclusions
These results indicate that ILCs and NK cells are important sources of inflammatory cytokines during acute dengue infection and display distinct profiles associated with different clinical forms.
Dietary dimethylglycine sodium salt supplementation improves growth performance, redox status, and skeletal muscle function of intrauterine growth-restricted weaned piglets
Authors: Bai Kaiwen , Jiang Luyi , Li Qiming , Zhang Jingfei , Zhang Lili , Wang Tian 
Published: June 2021

Abstract
Few studies have focused on the role of dimethylglycine sodium (DMG-Na) salt in protecting the redox status of skeletal muscle, although it is reported to be beneficial in animal husbandry. This study investigated the beneficial effects of DMG-Na salt on the growth performance, longissimus dorsi muscle (LM) redox status, and mitochondrial function in weaning piglets that were intrauterine growth restricted (IUGR). Ten normal birth weight (NBW) newborn piglets (1.53 ± 0.04 kg) and 20 IUGR newborn piglets (0.76 ± 0.06 kg) from 10 sows were obtained. All piglets were weaned at 21 d of age and allocated to the three groups with 10 replicates per group: NBW weaned piglets fed a common basal diet (N); IUGR weaned piglets fed a common basal diet (I); IUGR weaned piglets fed a common basal diet supplemented with 0.1% DMG-Na (ID). They were slaughtered at 49 d of age to collect the serum and LM samples. Compared with the N group, the growth performance, LM structure, serum, and, within the LM, mitochondrial redox status, mitochondrial respiratory chain complex activity, energy metabolites, redox status-related, cell adhesion-related, and mitochondrial function-related gene expression, and protein expression deteriorated in group I (P &lt; 0.05). The ID group showed improved growth performance, LM structure, serum, and, within the LM, mitochondrial redox status, mitochondrial respiratory chain complex activity, energy metabolites, redox status-related, cell adhesion-related, and mitochondrial function-related gene expression, and protein expression compared with those in the I group (P &lt; 0.05). The above results indicated that the DMG-Na salt treatment could improve the LM redox status and mitochondrial function in IUGR weaned piglets via the nuclear factor erythroid 2-related factor 2/sirtuin 1/peroxisome proliferator-activated receptorγcoactivator-1α network, thus improving their growth performance.
Water Balance and Desiccation Tolerance of the Invasive South American Tomato Pinworm
Authors: Tarusikirwa Vimbai L , Cuthbert Ross N , Mutamiswa Reyard , Gotcha Nonofo , Nyamukondiwa Casper 
Published: June 2021

Abstract
Temperature and dehydration stress are two major co-occurring environmental stressors threatening the physiology, biochemistry, and ecology of insects. As such, understanding adaptive responses to desiccation stress is critical for predicting climate change impacts, particularly its influence on insect invasions. Here, we assessed water balance and desiccation resistance of the invasive Tuta absoluta (Meyrick, 1917) (Lepidoptera: Gelechiidae), and infer how eco-physiology shapes its niche. We measured basal body water and lipid content, water loss rates (WLRs), and desiccation resistance in larvae (second to fourth instars) and adults. Body -water, -lipid, and WLRs significantly varied across life stages. Second instars recorded the lowest while fourth instars exhibited the highest body water and lipid content. Adult body water and lipid content were higher than second and third instars and lower than fourth instars while proportion of body water and lipid contents were highest in adults and second larval instars respectively. Water loss rates were significantly highest in fourth-instar larvae compared to other life stages, but differences among stages were less apparent at longer exposure durations (48 h). Desiccation resistance assays showed that second instars had greatest mortality while fourth-instar larvae and adults were the most desiccation tolerant. Our results show that T. absoluta fourth-instar larvae and adults are the most resilient developmental stages and potentially contribute most to the invasion success of the pest in arid environments. Incorporation of these species-specific eco-physiological traits in predictive models can help refine invasive species potential spread under changing climates.
The Cause of all Humanity
Authors: Eboe-Osuji Chile 
Published: June 2021

Abstract
After years of uneasy interactions between successive administrations of the United States and the International Criminal Court (ICC) — ranging from apathy to indifference to tacit utilitarian engagement behind the scenes — the administration of President Donald Trump unleashed the most intense aggression against the Court, marked by direct coercive measures against the Court’s officials. This followed relentless prior threats of such actions and promises to do more harm. Since other international institutions including the World Health Organization, the World Trade Organization, and the Human Rights Council have also been on the receiving end of rough treatment from Mr Trump, it is evident that the experience of the ICC under his administration was not entirely unique, although the particular circumstances may have been so. The new administration of Mr Biden has signalled an intention to reset US foreign policy — and has started this process — by reversing the more aggressive aspects of Mr Trump’s stance. In relation to the ICC, this change should go beyond merely reversing Mr Trump’s coercive measures. It should include positive support for the Court, given America’s leading role in inspiring its creation and the Court’s raison d’être which remains the cause of all humanity.
Discovery of an alternative pathway of peptidoglycan biosynthesis: A new target for pathway specific inhibitors
Authors: Ogasawara Yasushi , Dairi Tohru 
Published: June 2021

Abstract
Peptidoglycan in bacterial cell walls is a biopolymer consisting of sugars and amino acids and plays important role in maintaining cell integrity from the environment. Its biosynthesis is a major target for antibiotics and the genes and enzymes involved in the biosynthetic pathway have been well studied. However, we recently identified an alternative pathway in the early stage of peptidoglycan biosynthesis in Xanthomonas oryzae, a plant pathogen causing bacterial blight disease of rice. The distribution of the alternative pathway is limited to relatively few bacterial genera that contain many pathogenic species, including Xylella and Stenotrophomonas, besides Xanthomonas. Thus, the alternative pathway is an attractive target for the development of narrow-spectrum antibiotics specific to pathogens. In this minireview, we summarize the discovery of the alternative pathway and identification of its specific inhibitors.
Color Preference for Host-Seeking Activity ofAedes albopictusandCulex pipiens(Diptera: Culicidae)
Authors: Jung Sun Ho , Kim Dongmin , Jung Ki-Suck , Lee Dong-Kyu 
Published: June 2021

AbstractA cue for long-range vision allows mosquitoes to identify hosts and differentiate the ecological niches (e.g., habitats). However, the visual factors involved in attracting mosquitoes to a host are complex and have not been fully understood. Therefore, we assessed color preference to Aedes albopictus (Skuse) and Culex pipiens (Conquillett) as diurnal and nocturnal species, respectively, using seven fundamental colors including black, white, red, yellow, green, blue, and purple with each trap at 100 lux in a laboratory. We used a binary behavioral assay using the Mosquito Preference Index (MPI) as a preference ratio with a range of 0–1. Our analyses showed that Ae. albopictus had a greater response to black (MPIs, 0.7), followed closely by red, blue, and purple (MPIs, 0.6). We also found that red, blue, and purple were significantly higher (P &lt; 0.05) than those of green (MPI, 0.5), white (MPI, 0.3), and yellow (MPI, 0.2). Similarly, the MPIs for Cx. pipiens were significantly higher at black and red (MPIs, 0.7; P &lt; 0.05) compare to those of white and yellow (MPIs, 0.3; P &lt; 0.05). The color preference of Ae. albopictus showed significant correlation to luminous intensities (L-value) (r = −0.640; P = 0.000) and blue intensities (b-value) (r = −0.372; P = 0.000) for all seven colors. In addition, Cx. pipiens negatively correlated (r = −0.703; P = 0.000) between color preference and L-value. Our analyses provide a greater understanding of how color plays a role in visual sensory stimuli, and how that could potentially affect mosquito host-seeking behavior.
Alamandine protects against renal ischaemia–reperfusion injury in rats via inhibiting oxidative stress
Authors: Zhu Jue , Qiu Jian-Guo , Xu Wei-Tao , Ma Hong-Xiang , Jiang Ke 
Published: June 2021

Abstract

Objective
This study was to determine whether alamandine (Ala) could reduce ischaemia and reperfusion (I/R) injury of kidney in rats.

Methods
Renal I/R was induced by an occlusion of bilateral renal arteries for 70 min and a 24-h reperfusion in vivo, and rat kidney proximal tubular epithelial cells NRK52E were exposed to 24 h of hypoxia and followed by 3-h reoxygenation (H/R) in vitro.

Results
The elevated serum creatinine (Cr), blood cystatin C (CysC) and blood urea nitrogen (BUN) levels in I/R rats were inhibited by Ala treatment. Tumour necrosis factor alpha (TNF)-α, IL-1β, IL-6, cleaved caspase-3, cleaved caspase-8 and Bax were increased, and Bcl2 was reduced in the kidney of I/R rats, which were reversed by Ala administration. Ala reversed the increase of TNF-α, IL-1β, IL-6, cleaved caspase-3, cleaved caspase-8 and Bax and the decrease of Bcl2 in the H/R NRK52E cells. Ala could also inhibit the increase of oxidative stress levels in the kidney of I/R rats. NADPH oxidase 1 (Nox1) overexpression reversed the improving effects of Ala on renal function, inflammation and apoptosis of I/R rats.

Conclusion
These results indicated that Ala could improve renal function, attenuate inflammation and apoptosis in the kidney of I/R rats via inhibiting oxidative stress.
MHY1485 enhances X-irradiation-induced apoptosis and senescence in tumor cells
Authors: Sun Lue , Morikawa Kumi , Sogo Yu , Sugiura Yuki 
Published: June 2021

Abstract
The mammalian target of rapamycin (mTOR) is a sensor of nutrient status and plays an important role in cell growth and metabolism. Although inhibition of mTOR signaling promotes tumor cell death and several mTOR inhibitors have been used clinically, recent reports have shown that co-treatment with MHY1485, an mTOR activator, enhances the anti-cancer effects of anti-PD-1 antibody and 5-fluorouracil. However, it remains unclear whether MHY1485 treatment alters the effects of radiation on tumor cells. In this study, the radiosensitizing effects of MHY1485 were investigated using murine CT26 and LLC cell lines. We examined mTOR signaling, tumor cell growth, colony formation, apoptosis, senescence, oxidative stress, p21 accumulation and endoplasmic reticulum (ER) stress levels in cells treated with MHY1485 and radiation, either alone or together. We found that MHY1485 treatment inhibited growth and colony formation in both cell lines under irradiation and no-irradiation conditions, results that were not fully consistent with MHY1485’s known role in activating mTOR signaling. Furthermore, we found that combined treatment with MHY1485 and radiation significantly increased apoptosis and senescence in tumor cells in association with oxidative stress, ER stress and p21 stabilization, compared to radiation treatment alone. Our results suggested that MHY1485 enhances the radiosensitivity of tumor cells by a mechanism that may differ from MHY1485’s role in mTOR activation.
Mechanisms of stress response in the root stem cell niche
Authors: Ubogoeva Elena V , Zemlyanskaya Elena V , Xu Jian , Mironova Victoria 
Published: June 2021

Abstract
As plants are sessile organisms unable to escape from environmental hazards, they need to adapt for survival. The stem cell niche in the root apical meristem is particularly sensitive to DNA damage induced by environmental stresses such as chilling, flooding, wounding, UV, and irradiation. DNA damage has been proven to cause stem cell death, with stele stem cells being the most vulnerable. Stress also induces the division of quiescent center cells. Both reactions disturb the structure and activity of the root stem cell niche temporarily; however, this preserves root meristem integrity and function in the long term. Plants have evolved many mechanisms that ensure stem cell niche maintenance, recovery, and acclimation, allowing them to survive in a changing environment. Here, we provide an overview of the cellular and molecular aspects of stress responses in the root stem cell niche.
Jitter radiation: towards TeV-photons of gamma-ray bursts
Authors: Mao Jirong , Wang Jiancheng 
Published: June 2021

ABSTRACT
The synchrotron mechanism has the radiation limit of about 160 MeV, and it is not possible to explain the very high energy (VHE) photons that are emitted by high-energy objects. Inverse Compton scattering as a traditional process is applied for the explanation of the VHE emission. In this paper, jitter radiation, the relativistic electron radiation in the random and small-scale magnetic field, is proposed to be a possible mechanism to produce VHE photons. The jitter radiation frequency is associated with the perturbation field. The spectral index of the jitter radiation is dominated by the kinetic turbulence. We utilize the jitter radiation to explain the gamma-ray burst (GRB 190114C and GRB 180720B) VHE emissions that were recently detected by the Imaging Atmospheric Cherenkov Telescopes. We suggest that this mechanism can be applied to other kinds of VHE sources.
A 21-cm power spectrum at 48 MHz, using the Owens Valley Long Wavelength Array
Authors: Garsden H , Greenhill L , Bernardi G , Fialkov A , Price D C , Mitchell D , Dowell J , Spinelli M , Schinzel F K 
Published: June 2021

ABSTRACT
The Large-aperture Experiment to detect the Dark Age (LEDA) was designed to measure the 21-cm signal from neutral hydrogen at Cosmic Dawn, z ≈ 15–30. Using observations made with the ≈ 200 m diameter core of the Owens Valley Radio Observatory Long Wavelength Array (OVRO–LWA), we present a 2D cylindrical spatial power spectrum for data at 43.1–53.5 MHz (zmedian ≈ 28) incoherently integrated for 4 h, and an analysis of the array sensitivity. Power from foregrounds is localized to a ‘wedge’ within k⊥, $k_\parallel$ space. After calibration of visibilities using five bright compact sources including Vir A, we measure Δ2(k) ≈ 2 × 1012 mK2 outside the foreground wedge, where an uncontaminated cosmological signal would lie, in principle. The measured Δ2(k) is an upper limit that reflects a combination of thermal instrumental and sky noise, and unmodelled systematics that scatter power from the wedge, as will be discussed. By differencing calibrated visibilities for close pairs of frequency channels, we suppress foreground sky structure and systematics, extract thermal noise, and use a mix of coherent and incoherent integration to simulate a noise-dominated power spectrum for a 3000 h observation and z = 16−37. For suitable calibration quality, the resulting noise level, Δ2(k) ≈ 100 mK2 (k = 0.3 Mpc−1), would be sufficient to detect peaks in the 21-cm spatial power spectrum due to early Ly-α and X-ray sources, as predicted for a range of theoretical model parameters.