bluuebunny/crossref_metadata_2025_split huggingface dataset:
Perhexiline Therapy in Patients with Type 2 Diabetes: Dissociation Between Potentiation of Nitric Oxide Signalling and Changes in Insulin Resistance
Authors: Chong Cher-Rin , Liu Saifei , Imam Hasan , Heresztyn Tamila , Sallustio Benedetta , Chirkov Yuliy Y , Horowitz John D 
Published: December 2021

Abstract
Purpose: Perhexiline (Px) has previously been utilized primarily in the treatment of otherwise refractory angina pectoris and/or systolic heart failure. In recent years, Px has also shown increasing promise as a potential chemotherapeutic agent. Px inhibits carnitine palmitoyltransferases 1 and 2 (CPT1, CPT2), which control uptake of long-chain fatty acids into mitochondria and thus represent the rate-limiting steps in their metabolism. However, occasional cases of hypoglycaemia have been reported in Px-treated patients, raising the possibility that Px may also increase sensitivity to insulin. Furthermore, Px increases anti-aggregatory responses to nitric oxide (NO), an effect which may parallel insulin sensitization. No previous studies have examined either the effect of Px on insulin sensitivity, or the relationship of such putative changes with effects on NO signalling. We therefore sought to examine these relationships in patients with stable T2D and cardiovascular disease.Methods: In 30 patients with concomitant T2D and cardiovascular disease, Px was initiated, and dosage was titrated to reach therapeutic range and to prevent toxicity. Investigations were performed before and after 2 weeks, to examine changes in insulin sensitivity, platelet responsiveness to the anti-aggregatory effects of the NO donor sodium nitroprusside (SNP), as well as other markers of inflammation and modulators of NO signaling.Results: Px substantially potentiated inhibition of ADP-induced platelet aggregation by SNP (from 16.7±3.0 to 27.3±3.7%; p=0.005). Px did not change fasting blood glucose concentrations and reduced insulin sensitivity (HOMA-IR score increased from median of 4.47 to 6.08; p=0.028), via increasing fasting plasma insulin concentrations (median 16.5 to 19.0 mU/L: p=0.014). Increases in SNP responses tended (r=-0.30; p=0.11) to be reciprocally related to increases in HOMA-IR. No patient developed symptomatic hypoglycaemia, nor was there any other short-term toxicity of Px.Conclusions: In patients with stable T2D and cardiovascular disease, Px is not an insulin sensitizer, and does not normally induce hypoglycaemia. These results effectively dissociate the NO-sensitizing effect of Px from variability in insulin signaling.
Diversity of satDNA fraction in Cestrum, the Genus with the Largest Genomes within Solanaceae
Authors: de Souza Thaissa Boldieri , Parteka Leticia Maria , de Assis Rafael , Vanzela Andre Luis Laforga 
Published: December 2021

Abstract
Cestrum species present large genomes (~24 pg), a high occurrence of B chromosomes, and great diversity in heterochromatin bands. Despite this, there is maintenance of chromosome shape and karyotype symmetry. To deepen our knowledge on Cestrum genome composition, low coverage sequencing data of C. strigilatum and C. elegans were compared. Bioinformatics analyses showed retrotransposons comprising more than 70% of the repetitive fraction, followed by transposons (~18%). The four satDNA families that accumulated the most in the datasets were used as probes in FISH assays, and showed different distribution profiles along chromosomes. Most hybridization signals were located in the C-CMA/DAPI banding sites, including those related to AT-rich Cold-Sensitive Regions (CSRs) and heterochromatin. Although satellite probes hybridized in all tested species, a satDNA family named CsSat49 was highlighted as it predominates in centromeric regions. Data suggest that the satDNA fraction is still conserved in the genus, although there is variation in the number of FISH signals between karyotypes, as well as in the B chromosomes. This study brings an important advance in the knowledge on genome organization and heterochromatin composition in Cestrum, especially on the distribution and differentiation mechanisms of satellite fraction between species of a genus of Solanaceae with large genomes.
Analysis of Metal Transfer and Weld Forming Characteristics in Triple-wire Gas Indirect Arc Welding
Authors: Liu Liming , Wang Zeli , Zhang Tianyi , Ba Xianli 
Published: December 2021

Abstract
Triple-wire gas indirect arc welding (TW-GIA) has the advantages of low heat input and high deposition rate. However, the simultaneous melting of triple wires makes the metal transfer mode complicated. The unknown of the metal transfer mode restricts the development of this technology. In this paper, high-speed camera systems and electrical signal acquisition sensors were used to explore the TW-GIA metal transfer mode. The static force model and the arc conductive channel model were used to discuss the droplet force and energy conversion characteristics respectively. Results showed that the TW-GIA metal transfer modes can be divided into: short-circuit transfer (SCT), main wire projected transfer + side wire globular transfer (PGT), main wire streaming transfer + side wire projected transfer (SPT) and main wire streaming transfer + side wire streaming transfer (SST). Moreover, the process parameter ranges corresponding to the four modes were summarized. Due to the stable arc and the uniform metal transfer process, SPT and SST can form desirable weld seam. The gravity and z-axis components of electromagnetic force are the main forces that promote metal transfer. The x-axis and y-axis components of the electromagnetic force deviate the metal transfer path from the arc coverage. Due to the change of arc conductive channel, the energy transferred from TW-GIA to the base metal is less than that of GMAW, showing the advantages of small welding deformation, narrow heat affected zone and grain refinement.
Comparison of Outcomes in Obese Patients After Total Knee Arthroplasty With Neutral or Mild Varus: a Retrospective Study With 8-year Follow-up
Authors: Lai Ya-hao , Cao Jian , Feng Wei , Li Ze-xi , Xu Hong , Zhou Zongke 
Published: December 2021

Abstract

Background Residual varus after total knee arthroplasty (TKA) can affect functional outcomes and prosthetic survival, which may worsen in the presence of obesity. However, no studies were found to compare the outcomes of obese patients involving postoperative residual mild varus or neutral. The aim of this study was to compare outcomes for knees of obese patients with neutral or mild varus after TKA. Methods We retrospectively reviewed 188 consecutive obese patients (body mass index ≥ 30 kg/m2) at our hospital who underwent TKA due to varus knee osteoarthritis from January 2010 to December 2015. The mechanical hip-knee-ankle axis angle was measured in all patients pre- and postoperatively. Prosthesis survival and knee function were retrospectively assessed based on the Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC) score, Knee Society Knee Score (KS-KS), Knee Society Function Score (KS-FS), Forgotten Joint Score (FJS), and range of motion (ROM). Results Of the 156 knees in 132 obese patients who completed follow-up for a mean of 8.32 ± 1.47 years, 97 knees were corrected from varus to neutral and 54 knees were kept in mild residual varus. Knees with mild varus showed significantly better WOMAC and FJS scores. The two types of knees did not differ significantly in KS-KS, KS-FS or ROM. Although one patient with a neutral knee had to undergo revision surgery, there was no significant difference between two groups.Conclusions For obese patients with osteoarthritis, preservation of residual varus alignment after TKA can improve functional outcomes without compromising prosthesis survival.
Experimental Investigation on the Milling Performance of a 50 μm D-Shaped Micromilling Cutter with Different Materials
Authors: Jiang Chen , Jiang Jinxin , Hao Yu , Gao Rui , Zhang Yongbin 
Published: December 2021

Abstract
In micromilling, the performance and diameter of the milling cutter directly determine the service life of the milling cutter and the surface quality of the microgroove. Therefore, it is vital for high-precision milling to explore the milling performance of different materials and expand the application scope of micromilling cutter sizes. In this study, the milling performance of three kinds of material cutters—carbide, diamond coating and polycrystalline diamond (PCD)—was evaluated. A series of micromilling tests were carried out to determine the effects of cutter material type on cutter wear, surface quality and burr formation, particularly when a 50 µm micromilling cutter was used in the milling process. A D-shaped milling cutter with a diameter of 50 µm was manufactured on a self-developed high-precision modular machine tool by wire electrode electric discharge grinding (WEDG) technology. From theoretical and experimental perspectives, it is easy to master microgroove quality milled by different material cutters. The results show that the microgrooves processed with PCD cutters have fewer burrs, lower surface roughness values, and a smoother groove bottom morphology.
SPP1 is a Prognostic Related Biomarker and Correlated with Tumor-Infiltrating Immune Cells in Hepatocellular Carcinoma
Authors: Wang Xiaofeng , Zhang Kun , Geng Li , Liu DongLi 
Published: December 2021

Abstract
Background: Secreted phosphoprotein 1 (SPP1) functions as a tumor promoter in varies tumors, but little is known whether it is an actual player on driving immune infiltration in hepatocellular carcinoma. Methods: In this study, we identified the expression of SPP1 by Oncomine, GEPIA and TIMER databases, and assessed SPP1 immumohistochemical staining analysis by The HPA database. We evaluated the clinical outcomes between SPP1 expression and hepatocellular carcinoma patients via Kaplan-Meier Plotter. We also tested the relationship between SPP1 and critical oncogenes by TIMER and GEPIA databases. Then we explored immune infiltration analyses using TIMER and TISIDB datasets. In addition, we performed functional enrichment analyses with Metascape and GeneMANIA databases. Results: We found that SPP1 overexpressed in hepatocellular carcinoma tissues and high SPP1 expression was correlated with shorter OS and PFS survivals in hepatocellular carcinoma patients. SPP1 expression is positive correlation with critical oncogenes related stemness associated genes, cell cycle and proliferation, therapeutic resistance, metastasis, and tumor angiogenesis in hepatocellular carcinoma. Importantly, SPP1 expression was positively correlated with infiltrating levels of CD4+ T cells, CD8+ T cells, macrophages, neutrophils, and dendritic cells. Furthermore, SPP1 expression showed strong correlations with diverse immune hallmark sets in hepatocellular carcinoma. Notably, functional enrichment analysis suggested that SPP1 strong related with immune response. Conclusions: These findings imply that SPP1 is correlated with prognosis and immune cell infiltrating, offering a new potential immunotherapeutic target in hepatocellular carcinoma.
Efficient Organization of The Diagnostic Process In The Emergency Department of Patients With Acute Appendicitis During The COVID-19 Pandemic
Authors: Cybulska Natalia , Łopaciński Tomasz , Sieminski Mariusz 
Published: December 2021

Abstract
Background. The COVID-19 pandemic has forced significant changes in the functioning of health care systems worldwide. The availability of health resources and patient readiness to present to the hospital have been reduced. COVID-19-related safety procedures might have potentially disturbed diagnostic and therapeutic processes in the emergency department (ED), leading to noticeable delays in service with a possible negative impact on the final therapeutic outcome. The aim of this study was to assess whether changes in the health care system and designed modes of functioning of the ED during the pandemic had negative consequences on the diagnostic process and final effect of treatment of subjects with acute appendicitis.Methods. Due to the pandemic, certain changes in the functioning of emergency departments were implemented to ensure the safety of patients and the staff. We performed a retrospective analysis of the charts of patients diagnosed with acute appendicitis in the ED before (from 10.03.2019 to 31.12.2019) and during the pandemic (from 10.03.2020 to 31.12.2020). We compared the period from symptom onset to admission to the hospital, demographic and clinical features (including appendicitis severity), time intervals between crucial events in the diagnostic and therapeutic process and final results of therapy.Results. We included 133 patients (69 in the prepandemic period and 64 in the pandemic period). There were no significant differences in terms of demographic characteristics and comorbidities. During the pandemic, patients presented to the hospital later than before the pandemic, although the difference was not statistically significant (2.6 vs. 1.9 days from symptom onset, p=0.108). The severity of acute appendicitis was significantly higher during the pandemic according to the clinical scales used. For patients who underwent surgery, the time from admission to the beginning of the operation did not differ significantly before versus during the pandemic. We did not find significant differences in length of stay, rates of complications, reoperations or rehospitalizations between the prepandemic and pandemic periods.Conclusions. Our data show that COVID-19-related changes in the functioning of the ED did not lead to delays in the diagnostic process or to worsening of the therapeutic effect in patients with acute appendicitis.
Real Structure of Benzene Molecule and the Thickness of Graphene
Authors: Wang Dulun 
Published: December 2021

Abstract
The problem of benzene molecular structure has not been solved for more than 100 years. This research proposes a new concept of covalent bond based on the existing theory: each electron shared by multiple atom nuclei corresponds to a half-valent bond. The half-valent bond can be formed between the spacer carbon atoms of the benzene ring. In this way, a new theory was established. Quantum mechanical calculations can quantitatively explain experimental results, such as the hydrogenation heat and ultraviolet spectrum of benzene. Using the dotted line to indicate the half-valent bond, benzene molecular structural forms and chemical reaction formulas as will as its dozens of homologues and derivatives are designed easily. The method not only has a wide range of adaptability, but can also record the chemical reaction process. If several stacked benzene rings can form a benzene tube under the guidance of the new theory, calculated thickness of the three-layer benzene tube is very close to the thickness of graphene. Therefore, referring to other characteristics of graphene, it is considered to be more like a three-layer structure.
Clinical Features, Management and Prognostic Factors of Intracranial Solitary Fibrous Tumor
Authors: Liu Jingdian , Wu Sisi , Wang Junwen , Shu Kai , Lei Ting 
Published: December 2021

Abstract
Purpose Due to low incidence and the constantly changing diagnostic and classification criteria, the clinical features, management and prognostic factors of intracranial solitary fibrous tumor (ISFT) remain unclear and were thus analyzed in this study. Methods A total of 38 patients who were diagnosed as ISFT in our institution were enrolled in this study. Patient data including age, gender, clinical presentation, histopathological features, immunohistochemistry staining, tumor location, tumor size, treatment methods, and prognosis were extracted and retrospectively analyzed. Results The median age at diagnosis was 45.5 years (range 28-66 years) and the male to female ratio was 1:1.53 in our series. The 3-, 5-, and 10-year progression-free survival rate was 82.2%, 62.8%, and 21.4%, respectively; and the 3-, 5-, and 10-year overall survival rate was 97.1%, 86.9% and 64.2%, respectively. Patients with high WHO grade (grade III) ISFTs had impaired PFS (p<0.05) and OS (p<0.01). Subtotal resection (STR) was associated with shorter PFS and OS (p<0.001, respectively). Postoperative radiotherapy (PORT) significantly improved PFS but not OS in patients with WHO grade III ISFTs (P=0.025). The PFS of patients with STR+PORT was improved compared with those who received STR alone, although not significant. Moreover, CD34-negative immunostaining and high Ki-67 index (>10%) were associated with impaired PFS in ISFTs.Conclusion Our study provides evidence that high tumor grade, subtotal tumor resection, CD34 negative immunostaining and high Ki-67 index (>10%) were independent predictors for poor prognosis of ISFTs. PORT should be recommended for patients with high grade ISFTs or STR.
Helminths' antigens differentially modulate the activation of SARS-CoV-2-reactive helper and cytotoxic T cells in COVID-19 patients and healthy blood donors
Authors: Adjobimey Tomabu , Meyer Julia , Terkeš Vedrana , Parcina Marijo , Hoerauf Achim 
Published: December 2021

Abstract
Background

Contrary to the predictions, prevalence and mortality due to COVID-19 have remained moderate on the African continent. Several factors, including age, genetics, vaccines, and co-infections, might impact the course of the pandemic in Africa. Helminths are highly endemic in Sub-Saharan Africa and are renowned for their ability to modulate their host immune reactions.
Methods

Here we analyzed in vitro the impact of major helminth antigens on the immune reactivity to SARS-CoV-2 in COVID-19 patients using flow cytometry and Luminex.
Results:

We observed that helminth antigens significantly reduced the frequency of SARS-CoV-2-reactive CD4+ T helper cells. In contrast, the expression of SARS-CoV-2-reactive CD8+ T cells was not affected. In addition, stimulation with helminth antigens was associated with increased IL-10 and a reduction of IFNγ and TNFα.
Conclusion:

Our data offer a plausible explanation for the moderate incidence of COVID-19 in Africa and support the hypothesis that helper T cell-mediated immune responses to SARS-CoV-2 are mitigated in the presence of helminth antigens, while virus-specific cytotoxic T cell responses are maintained.
Assessment of Turnover Intention and Associated Factors Among Health Information Technology Professionals Working in Public Health Institution, South Gondar Zone, Northwest Ethiopia
Authors: Zimamu Libsuye Yalgaw , Wubet Gashaw Mehiret 
Published: December 2021

Abstract
Background: Place of work turnover is critical to health information technology professionals (HIT) and health information systems as it indications dropping skilled and competent health information professionals. However, the progress of surviving plans requests a strong acceptance of the place of work variables that either inspire health information staff to persist active or principal them to dispensation their recent working organization.Objective: This study was deliberate to measure intention to leave and associated factors among health information technology professionals working in south Gondar zone public health institution, Amhara Region, EthiopiaMethods: A cross-sectional study was carried out among 122 health information technology professionals. Data were collected using structured pre-tested self-administered questionnaires from September 1 to October 1/2020. The collected data were entered into Epi-info Version 7 and exported to SPSS version 20 software for analysis. Bivariate and multivariable logistic regression was employed to detect influences associated with dependent and independent variables. Odds ratio (OR), with 95% CI and p< 0.05 were computed to determine the level of significance.Results: Based on the operational definition the total score of intent to leave health information technology professionals from the organization was 64.8% [95% CI: 56.6-72.9]. Among the applicant variables for multivariable analysis 3 variables like the marital status of the respondents [OR: 0.49(0.231-1.042)p: value 0.000], performance appraisal of the respondents [OR: 7.0(2.66-18.45) p: value 0.000] and recognition of the respondents [OR: .447(.783-3.513) p: value 0.002] had to have a significant association with intention to leave and performance appraisal of the respondents were significantly associated 8.29 times [AOR: 8.29(3.002-22.889)p: value 0.001] to leave of the respondents from the working organization.Conclusion and recommendation: The magnitude of health information professionals' intention to leave was found to be high, which can extremely affect the coverage and quality of information management and use of information for decisions in the zone. Health care policy-makers and hospital managers need to develop and institutionalize evidence-based retention strategies taking into consideration the predictors of health information professionals’ intention to leave.
Effects of Differently Incubated Cupric Oxide Nanoparticles on the Granulosa Cells of Caprine Ovary in Vitro
Authors: Kumar Chetan , Sharma Rajnesh Kumar 
Published: December 2021

Abstract
Present study was aimed to investigate the effect of temperature on the shape and size of nanoparticles and related cytotoxicity of these particles on ovarian granulosa cells. Cupric oxide nanoparticles (CuONPs) were synthesized using a simple, efficient, and reproducible precipitation method involving reduction of Cu metal salt with sodium hydroxide and then incubation of the precipitates at 70oC for 5 hrs. Subsequently, this prepared sample was divided into 3 subsamples and incubated at 3 different temperatures i.e. 70oC, 150oC, and 350oC for a time duration of 5 hrs. to study the effect of temperature on the particles. The products were characterized by XRD, FTIR, HRTEM, and FESEM. Characterization of the particles revealed that all particles were monoclinic crystalline in nature and had a size range from 9 nm - 60 nm. Particles were of different shapes; spherical, needle, and capsule. Toxicity of each particle was determined on granulosa cells by exposing them for 24 hrs. at 2 different doses. Toxicological results showed the size and shape-related toxicity of nanoparticles; particles which were spherical shape were significantly more toxic than capsule-shaped particles.