bluuebunny/crossref_metadata_2025_split huggingface dataset:
Enpp1 ameliorates MAFLD by regulating hepatocyte lipid metabolism through the AMPK/PPARα signaling pathway
Authors: Liu Xiaohui , Chen Shuai , Liu Xing , Wu Xianxian , Jiang Xiaoliang , Li Yuhan , Yang Zhiwei 
Published: February 2025

Abstract

Background
Metabolic dysfunction-associated fatty liver disease (MAFLD) has become the leading chronic liver disease globally, and there are no approved pharmacotherapies to treat this disease. Ectonucleotide pyrophosphatase/phosphodiesterase 1 (Enpp1) has been found to be related to insulin resistance and lipid accumulation. However, the role and mechanism of Enpp1 in the development of MAFLD remain unknown.

Results
Here we discovered that Enpp1 is lowly expressed in the liver of MAFLD patients by clinical investigation. Knocking out Enpp1 in the liver of mice aggravated obesity, insulin resistance and hepatic steatosis, and these effects were reversed by liver-specific Enpp1 overexpression. Through transcriptomic data mining and experimental validation, we demonstrated that Enpp1 deficiency inhibited the expression of AMPK (energy receptor) and PPARα (nuclear transcription factor for lipid metabolism), thereby promoting the transcription of lipid synthesis factors and mediating the progression of MAFLD. Mechanistically, Enpp1 enhanced the activity of AMPK by increasing the AMP-to-ATP ratio, which in turn raised PPARα levels and promoted the transcription of its downstream lipid metabolism factors. Pharmacological inhibition of AMPK activity abolished the promoting effect of Enpp1 on PPARα protein expression.

Conclusions
This study indicate that Enpp1 can effectively ameliorate MAFLD through effects on AMPK/PPARα signaling pathway-mediated lipid metabolism, revealing the significance of Enpp1 as a promising therapeutic target against MAFLD.
Examining maladaptive eating behaviors and psychological difficulties among women with compulsive eating and obesity: a latent profile analysis
Authors: Maltais-Lévesque Charlie , Legendre Maxime , Bégin Catherine 
Published: February 2025

Abstract

Background
Maladaptive eating behaviors are central to weight gain and are influenced by various psychological determinants associated with obesity. While some studies have established profiles based on different maladaptive eating behaviors using medical samples of individuals with obesity, no studies have focused on all patients with overweight or obesity seeking psychological help for compulsive eating. The objective was to identify eating behaviors profiles using maladaptive eating behaviors (disinhibition, susceptibility to hunger, and restraint) among women patients with compulsive eating and overweight or obesity, and to compare those profiles with each other on socio-demographic, clinical, psychological, and eating-related variables.

Methods
One hundred and eighty-eight women patients with overweight or obesity (BMI ≥ 25 kg/m2) completed online questionnaires and participated in an eating disorders diagnosis interview. Profiles were created using latent profile analysis and compared with a MANOVA with Tukey adjusted post-hoc comparisons and a chi-square analysis.

Results
Three profiles emerged from the latent profile analysis. The Highly disinhibited profile includes women with high scores for disinhibition and susceptibility to hunger and a low score of restraint. The Moderate sensitivity to eating cues profile includes women with moderate scores of disinhibition, susceptibility to hunger, and restraint. The Perceived control over food profile includes women with the highest restraint score and the lowest levels of disinhibition and susceptibility to hunger. Group comparisons showed significant differences between profiles. The Highly disinhibited profile included women with higher levels of depressive symptoms and impulsivity as well as lower scores of self-directedness and cooperation. This profile also showed the highest level of binge eating, food craving, and food addiction symptoms. The Moderate sensitivity to eating cues profile showed more body esteem and greater cooperation. The Perceived control over food profile had less impulsivity and depressive symptoms as well as a higher level of self-directedness.

Conclusion
These results highlighted mechanisms that seem to prevail in different profiles of patients with compulsive eating which offer intervention targets that should be prioritized when offering psychotherapeutic treatment.
The impact of a personality trait rooted in Chinese confucian culture—Junzi personality—on loneliness and subjective well-being among chinese individuals: the mediating role of self-reflection and self-control
Authors: Zhao Boqiang , Zhang Yuhan 
Published: February 2025

Abstract
Reducing loneliness and enhancing subjective well-being are key topics in psychological research. However, few studies have explored the impact of cultural factors on loneliness and subjective well-being from a cultural perspective. Moreover, there has been few research examining the underlying mechanisms through which sociocultural factors influence individual loneliness and well-being. This study aims to explore the influence of a personality trait rooted in Chinese Confucian culture—Junzi personality—on subjective well-being among Chinese, specifically examining the mediating roles of self-reflection and self-control. A sample of 693 Chinese college students were surveyed on their Junzi personality, self-reflection, self-control, loneliness, and subjective well-being in three stages over 6 months. Correlation analysis and a multiple mediation model were conducted using SPSS 23.0 and AMOS 23.0. Results revealed that Junzi personality positively predicted subjective well-being and negatively predicted loneliness. Additionally, self-reflection, self-control, and loneliness sequentially mediated the effect of Junzi personality on subjective well-being. This study highlights the significant role of cognitive factors in the process through which sociocultural factors influence well-being.
A decade of research contributions and emerging trends in the International Journal of STEM Education
Authors: Chiu Thomas K. F. , Li Yeping , Ding Meixia , Hallström Jonas , Koretsky Milo D. 
Published: February 2025

Abstract
In this editorial, we review 400 articles and reviews published in the International Journal of STEM Education during its first decade (2014–2023). Using bibliometric analysis, we examine these publications to assess the journal’s major contributions to STEM education research and identify emerging trends over the years. The results present a dynamic picture of the growth of STEM education, highlighting key topics, such as STEM integration, equity, and emerging technologies. These findings also reveal evolving “hot topics” that reflect the shifting interests of researchers in the field. This review suggests that many areas of STEM education research are still in the growth phase. We encourage readers to use these insights as a foundation for developing future research agendas and advancing STEM education globally.
No disadvantages for women in acute stroke care in Germany: an analysis of access to stroke treatment services in Germany from 2017 to 2022
Authors: Ungerer Matthias N. , Bartig Dirk , Tunkl Christine , Richter Daniel , Katsanos Aristeidis , Krogias Christos , Hacke Werner , Gumbinger Christoph 
Published: February 2025

Abstract

Background
Several publications have raised concerns that female stroke patients may be at a disadvantage when accessing stroke treatment services. These publications have found significant regional differences in the provision of stroke treatment to male and female patients. In this study, we provide current nationwide data on stroke management differences between men and women in Germany.

Methods
This large retrospective cohort study used national datasets from the German Federal Statistical Office for 2017–2022. We examined differences between female and male stroke patients in terms of case volume, intravenous thrombolysis (IVT), mechanical thrombectomy (MTE), stroke unit (SU) treatment, intrahospital mortality, and prevalence of atrial fibrillation (AF).

Results
Data from more than 1.3 million hospitalised stroke patients were included. Forty-seven percent of the patients were female. Female patients were older and more frequently ≥ 80 years old (50.3% versus 29.4%). Rates of IVT (16.3% versus 16.3%) were similar for both sexes but higher in females when adjusted for age. MTE rates (8.2% versus 6.3%) were consistently higher in female patients across all age groups. Female patients had higher rates of intrahospital mortality (9.1% versus 6.2%), and admission to SUs (73.6% versus 76.0%) was less common. Treatment rates in intensive care units were similar (10.6% versus 10.5%). AF, a surrogate for embolic (and more severe) strokes, was more prevalent in females (32.6% versus 25.4%).

Conclusions
We found no evidence that female stroke patients in Germany face any disadvantage in accessing stroke treatment services. Acute stroke treatment rates were generally similar or higher when compared to males. Higher intrahospital mortality and lower SU rates were attributed to greater age, comorbidities, and stroke severity. However, the differences were not fully explained when adjusting for AF and age. Further research is needed on sex differences in stroke mechanisms and outcomes.
Developing a one health data integration framework focused on real-time pathogen surveillance and applied genomic epidemiology
Authors: Oltean Hanna N. , Lipton Beth , Black Allison , Snekvik Kevin , Haman Katie , Buswell Minden , Baines Anna E. , Rabinowitz Peter M. , Russell Shannon L. , Shadomy Sean , Ghai Ria R. , Rekant Steven , Lindquist Scott , Baseman Janet G. 
Published: February 2025

Abstract

Background
The One Health approach aims to balance and optimize the health of humans, animals, and ecosystems, recognizing that shared health outcomes are interdependent. A One Health approach to disease surveillance, control, and prevention requires infrastructure for coordinating, collecting, integrating, and analyzing data across sectors, incorporating human, animal, and environmental surveillance data, as well as pathogen genomic data. However, unlike data interoperability problems faced within a single organization or sector, data coordination and integration across One Health sectors requires engagement among partners to develop shared goals and capacity at the response level. Successful examples are rare; as such, we sought to develop a framework for local One Health practitioners to utilize in support of such efforts.

Methods
We conducted a systematic scientific and gray literature review to inform development of a One Health data integration framework. We discussed a draft framework with 17 One Health and informatics experts during semi-structured interviews. Approaches to genomic data integration were identified.

Results
In total, 57 records were included in the final study, representing 13 pre-defined frameworks for health systems, One Health, or data integration. These frameworks, included articles, and expert feedback were incorporated into a novel framework for One Health data integration. Two scenarios for genomic data integration were identified in the literature and outlined.

Conclusions
Frameworks currently exist for One Health data integration and separately for general informatics processes; however, their integration and application to real-time disease surveillance raises unique considerations. The framework developed herein considers common challenges of limited resource settings, including lack of informatics support during planning, and the need to move beyond scoping and planning to system development, production, and joint analyses. Several important considerations separate this One Health framework from more generalized informatics frameworks; these include complex partner identification, requirements for engagement and co-development of system scope, complex data governance, and a requirement for joint data analysis, reporting, and interpretation across sectors for success. This framework will support operationalization of data integration at the response level, providing early warning for impending One Health events, promoting identification of novel hypotheses and insights, and allowing for integrated One Health solutions.
The long-term effect of SiO2 nanoparticles in drinking water inducing chronic kidney disease in a mouse model with modern dietary habits
Authors: Ye Tzung Cheng , Chao Shu-Ju , Huang Chihpin 
Published: February 2025

Abstract
SiO2 nanoparticles (SNPs), which reportedly have low potential side effects, are used in various applications, for example, as food additives, for drug delivery, and as semiconductors, but these NPs are a growing concern because a few studies have shown potential risks to human health, such as fatty liver formation. In recent years, dietary habits have changed, and more refined foods, sugary beverages, and fast food are being increasingly consumed during daily life; therefore, the dietary habits have changed toward increasing consumption of high-fat diets, which may increase the incidence of liver and kidney disease. Our in vivo study demonstrated that the administration of SNPs in drinking water in combination with a high-fat diet was associated with the accumulation of SNPs and triglycerides in renal tissue; elevations in the blood urea nitrogen, uric acid, and creatinine levels in serum; the formation of basophilia and tubular cell sloughing; abnormal renal parenchyma, cysts, and hyaline casts; the induction of proliferating cell nuclear antigen and markers for chronic kidney disease such as vimentin, lipocalin 2, kidney injury molecule; the activation of fibrosis and inflammation; increased oxidative stress; and the development of chronic kidney disease. The evidence showed that long-term treatment of SNPs in drinking water might induce renal injury in individuals with modern dietary habits and provided important results for evaluating the safety of SNPs, especially for human health.
Protein disulfide isomerase in Philadelphia-negative myeloproliferative neoplasms: a case–control study
Authors: Mohammed Marwa Salah , Alnaggar Alaa , Tawfik Nehad , Morad Mohamed Abdelkader , Abdelaziz Eman Ahmed , Yasser Nagham , Soliman Lamyaa H. , Elshenoufy Mai Galal 
Published: February 2025

Abstract

Background
Classic Philadelphia-negative myeloproliferative neoplasms (MPNs), such as polycythemia vera (PV), essential thrombocythemia (ET), and myelofibrosis (MF), are defined by the unregulated production of bone marrow components resulting from the clonal expansion of multipotent bone marrow progenitors. Thrombotic complications represent a major cause of morbidity and mortality in BCR-ABL-negative MPNs. Multiple factors have been identified as potential contributors to thromboembolism in MPN patients, yet the exact pathophysiology remains poorly understood. Also, the current risk stratification systems lack early predictors of thromboembolic risk, mandating the need to identify new surrogate thrombotic markers. Despite the use of cytoreductive therapy and antithrombotic drugs, unfortunately, the incidence rate of thrombosis remains high, which advocates for further research to discover novel antithrombotic therapeutic targets. Protein disulfide isomerase (PDI), an endoplasmic reticulum protein that is critical for protein folding, is released by stimulated platelets and endothelial cells and was found to play a regulatory role in thrombosis. Inhibitors of PDI have been shown to prevent thromboembolic events in cancer patients. We aimed to study the PDI level in MPN patients and assess its role as a marker of increased thromboembolic risk in MPN patients, which might shed light on a new area of investigation in the prognosis and prevention of thromboembolism in MPN patients.

Methods
This is a case–control study where we investigated the serum level of PDI in a cohort of MPN patients in comparison to a control group. Serum PDI level was measured using ELISA to assess if PDI level is pathologically elevated in MPN patients; we also correlated the PDI level with either history/recent thrombotic events or the presence of silent thrombosis screened for by venous and arterial duplex.

Results
PDI levels were found to be pathologically elevated in MPN patients when compared to the control group. As a secondary outcome, we aimed to correlate the level of PDI to different clinical and imaging parameters, but no significant association could be found.

Conclusion
PDI may have a possible role in the underlying pathogenesis of thrombotic events in Philadelphia-negative MPN patients and may have a role in predicting a higher risk of developing thromboembolic events, and this may shed light on the possible future use of PDI as a therapeutic target for prevention of thrombosis in MPN patients.
High ionized calcium in the general population and risk of primary hyperparathyroidism
Authors: Kobylecki Camilla J , Nordestgaard Børge G , Afzal Shoaib 
Published: February 2025

Abstract

Background
Although it is generally held that in otherwise healthy individuals, primary hyperparathyroidism is the most likely cause of persistent hypercalcemia, solid research supporting this assumption is absent. We aimed to examine the relative and absolute risk of primary hyperparathyroidism associated with an incidental high ionized calcium in the general population.

Methods
We used the Danish Copenhagen General Population Study, a prospective cohort study with inclusion in 2003-2015, to investigate the association of ionized calcium on continuous and categorical scales with primary hyperparathyroidism using Cox regression and competing-risk regression. Hazard ratios and absolute 10-year risks of primary hyperparathyroidism were calculated.

Results
In 106,588 individuals, 2,497(2.5%) had moderately high (1.33-1.40 mmol/L) or high (&gt;1.40 mmol/L) plasma ionized calcium at baseline and 441(0.4%) were diagnosed with primary hyperparathyroidism during follow-up. The multivariable adjusted hazard ratios for primary hyperparathyroidism for moderately high and high plasma ionized calcium versus low normal ionized calcium were 65(95%CI: 46‒92) and 350(251‒ 489). Stratified on sex, the corresponding hazard ratios were 63(42‒95) and 326(221‒482) for women and 73(37‒145) and 490(256‒935) for men. For women and men above 65 years, absolute risks of primary hyperparathyroidism were 7.9% and 3.3% in those with moderately high plasma ionized calcium, and 44% and 21% in those with high plasma ionized calcium.

Conclusions
High ionized calcium found in 1:40 in the general population conferred absolute 10-year risks of primary hyperparathyroidism of up to 44%. These findings support further diagnostic work-up following an incidentally observed high plasma ionized calcium in otherwise healthy individuals.
Prevalence and Management of Complications of Laser Ablation for Benign Thyroid Nodules: A Systematic Review of Literature and Meta-analysis
Authors: Scappaticcio Lorenzo , Di Martino Nicole , Ferrazzano Pamela , Maiorino Maria Ida , Caruso Paola , Volatile Alessandra , Longo Miriam , Docimo Giovanni , Iervolino Eduardo , Trimboli Pierpaolo , Esposito Katherine , Bellastella Giuseppe 
Published: February 2025

Abstract

Context
Percutaneous laser ablation (LA) is today regarded as a valuable therapy for symptomatic, benign solid (non-cystic) thyroid nodules.

Objective
We assessed the prevalence of complications from laser ablation (LA) for benign and solid thyroid nodules (STNs) and their management.

Methods
We conducted a systematic review with meta-analysis of data from published studies on LA of STNs, in addiction to author’s institution. A random effects meta-analysis was performed on the prevalence rates.

Results
The literature search yielded 1351 studies, of which 38 studies were included, in addition to our institutional experience (4745 STNs in total). The overall quality of each included study was judged as fair. The prevalence of “Overall” complications of LA was 23% ([CI 17–30%], I2 93.7%, 1208 of 4702 thyroid nodules [TNs]). The prevalence of “Minor” complications of LA was 21% ([CI 15–27%], I2 93.7%, 1159 of 4702 TNs). The prevalence of “Major” complications of LA was 2% ([CI 1–3%], I2 54.0%, 49 of 4745 TNs). Sensitivity analyses did not modify the results, except for dysphonia whose pooled prevalence was higher when using local anesthesia (2%, CI [1-3], I2 25.2, p-value 0.010) or conscious sedation (2%, CI [1-4], I2 27.2, p-value 0.014). The pooled prevalence rate of local pain was 15% (CI [12-20], I2 89.3). Local pain was transient and typically mild to moderate, sometimes severe requiring analgesics for 1-5 days up to one month. The pooled prevalence rate of dysphonia was 2% (CI [1-2], I2 30.3). All cases of dysphonia were transient except for one permanent case.

Conclusion
LA for benign and solid (non-cystic) thyroid nodules could be considered a generally safe technique. Major complications were rare.
Are child field student nurses psychologically prepared for their first-year clinical placements?
Authors: Couzens Chloe , Osewa Afusat 
Published: February 2025

Background: The impact of the first-year clinical placement on the mental health and wellbeing of nursing students remains under-researched, with limited information on how well students are initially prepared for clinical placement both mentally and emotionally, specifically child field student nurses (CFSN). Aim: Understand the psychological preparedness of CFSNs for their first-year clinical placements. Method: Using a phenomenological approach, this study aims to understand the experiences of CFSNs in the context of their psychological preparedness for their first-year clinical placements. Findings: Psychological preparedness concerning clinical placement is not a singular concept, but comprises feeling both practically and emotionally ready for the opportunities, challenges and overall experiences that clinical areas offer. Conclusion: Higher education institutions need to better prepare and protect the psychological wellbeing of CFSNs in their first year. This can be achieved through an enhanced curriculum and developing simulated practice sessions that better reflect the clinical environment.