bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification of restriction enzyme in the FSHR gene of indonesian local cattle
Authors: Prihandini P W , Primasari A , Luthfi M , Pamungkas D , L Sari A P Z N , Dina T B , Maharani D 
Published: November 2021

Abstract
The restriction enzyme is important for genotyping using the PCR-RFLP technique. Therefore, this study aims to identify the restriction enzyme mapping in the partial sequence of the follicle-stimulating hormone receptor (FSHR) gene in Indonesian local cattle. A total of 29 samples sized 306 bp, were aligned with Genbank sequence acc no. NC_032660, resulting three polymorphic sites, namely g.193G>C, g.227T>C, and g.275A>C. Furthermore, the restriction mapping analysis using the NEBcutter program V2.0 showed that no enzyme recognized the SNP g.275A>C, while the SNP g.193G>C and g.227T>C were identified by the AluI and MscI enzymes, respectively. The AluI enzyme cuts at two positions (193 bp and 243 bp) in the G allele sample producing three fragments namely 50 bp, 63 bp, and 193 bp, meanwhile, in the C allele, the AluI cuts only in position 243 bp, hence, the fragment products are 63 bp and 243 bp. In contrast, the MscI enzyme was only recognized in the T allele, producing fragments sized 77 bp and 229 bp but failed to identify the restriction site along with the PCR products in the C allele. Based on the results, the SNPs (g.193G>C and g.227T>C) and restriction enzymes (AluI and MscI) are applicable for genotyping local Indonesian cattle using the PCR-RFLP technique in future studies.
Effect of different queen cell sizes on the acceptance of grafted larvae in Apis cerana javana Fabr. Rearing
Authors: Ustadi  , Suyadi  , Ikhsan N , Radiati L E , Sjofjan O , Batoro D , Susilo A 
Published: November 2021

Abstract
This study aims to evaluate Apis cerana javana Fabr. rearing based on differences in the queen cup sizes. A total of four queen bees were used with three different treatments and six replications, meanwhile, this study was conducted at Kembang Joyo Bee Farm, Malang, East Java. The results showed that the differences in the queen cup size had no significant effect on the success rate of queen rearing (p>0.05). The highest percentage of larvae acceptance (79%) was found in P3 treatment, followed by P2 (71%), while the lowest was found in P1 (63%). Furthermore, the highest percentage of larvae to pupae metamorphosis was found in P1 and P2 with a success rate of 100%, while the lowest was found in P3 with 89%. The highest percentage of pupae emergence was found in treatment P1 and P2 with a success rate of 100% and P3 with 94%. Based on the results, the difference queen cup sizes had no significant effect on the success rate of Apis cerana javana Fabr. rearing.
Physico-chemical properties of gathot (fermented cassava) flour applied on edible film
Authors: Ratnaduhita A , Nuhriawangsa A M P , Kartikasari L R 
Published: November 2021

Abstract
The objective of this research is to examine the physicochemical properties of gathot and its potential as an edible film. The materials used included dried gathot, carrageenan, and glycerol with 4 levels of treatment and 5 repetitions. The treatment was in the form of variation of gathot flour’s concentration (0.00; 0.75; 1.00; 1.25 %). The test was conducted on gathot flour, covering scavenging ability, bulk density, water absorbency, color brightness, proximate analysis and crude fiber with descriptive analysis. Gathot flour-derived edible film’s characteristics were tested for tensile strength and water vapor transmission rate (WVTR) to examine whether gathot flour-derived edible film was able to compete with commercial bioplastics through quantitative statistical analysis and Completely Randomized Design (RAL) and processing with SPSS 23.0. The results of physicochemical test on gathot flour were scavenging ability 49.37%; bulk density 0.57 g/mL; water absorbency 2.37 g/g; color brightness 17.42; water content 9.32%; protein content 4.76%; fat content 0.13%; ash content 0.09%; carbohydrate 85.70%; and crude fiber 3.54%. The average results of edible film’s characteristics test with a variation of gathot flour’s concentration were tensile strength 1.41-3.00 N/mm2 and water vapor transmission rate (WVTR) 5.42-6.99%. From the perspective of physicochemical properties, it is concluded that gathot flour is almost equal to wheat flour and tapioca flour. In addition, gathot flour-derived edible film at up to concentration level 1.25% has met the criteria for the edible film with moderate properties and complied with the standard bioplastic packaging.
Optimum condition of Streptococcus termophilus, Lactobacillus fermentum, and Lactobacillus plantarum producing yoghurt starter
Authors: Sukma A , Anggraini O R , Kurnia Y F , Purwati E 
Published: November 2021

Abstract
The study aimed to reveal the interaction of temperature and duration of incubation onto total LAB yogurt starter producer. The bacteria inoculated was used are Streptococcus termophilus, Lactobacillus fermentum, and Lactobacillus plantarum. The research method was used are randomized block factorial design was A factor variety of temperature A1 (25 0C), A2 (30 0C), A3 (37 0C), and A4 (42 0C), then B factor range of duration of incubation B1 (12 h), B2 (18 h) and B3 (24 h) within three replications. Best treatment determined by the highest Lactic Acid Bacteria (LAB) level consisted of treatment. Only the best treatment will analyze the proximate. The result showed an interaction between temperature and duration onto total LAB on the yogurt starter producer. It can be concluded that the best results from starter yogurt using three bacterial cultures were obtained at an incubation temperature of 370C for 18 hours with the number of LAB is 5,5 x 1010. Proximate results are the pH value of 4.46, Total Titrations Acid 2.20%, the water content of 82.48%, the protein content of 6.39%, and fat content of 4.36%.
Potential of Indigenous Microorganisms (IMO) from cow manure, elephant grass, Aloe vera, pineapple skin to the N, P and K content of organic cow manure
Authors: Novia D , Sandra A , Yogi A , Allismawita  , Putra A A 
Published: November 2021

Abstract
The manufacture of organic fertilizers can be accelerated by adding indigenous microorganisms (IMO) which function as decomposers. The purpose of this study was to determine the effect of adding several types of IMO to the N, P, and K content of organic cow manure. This research used animal MOL, namely cow manure IMO and vegetable IMO, namely elephant grass IMO, Aloe vera IMO, pineapple skin IMO and dry cow manures. This study used an experimental method with a completely randomized design with five treatments and four replications. The treatments were A: addition of cow manure IMO, B : elephant grass IMO, C : Aloe vera IMO, D : pineapple skin IMO, E : combination IMO (A, B, C, D). The parameters were measured; the N, P and K content. The results showed that the addition of several types of IMO had a significant effect (P<0.05) on phosphorus content of organic cow manure but had no significant effect (P>0.05) on nitrogen and potassium content. The manufacture of organic cow manure with the addition of several types of IMO for 21 days gave the best results in the combination IMO treatment with 1.70% nitrogen, 0.65% phosphorus, and 1.78% potassium content.
Variant alpha and beta biodiversity of the genus in dadiah through deep sequencing 16S Ribosomonal RNA genes
Authors: Fatdillah H , Desriani  , Melia S , Fitria N , Morita H , Sukma A 
Published: November 2021

Abstract
Dadiah is a traditional spontaneous fermentation of buffalo milk from West Sumatra that doesn’t go through pasteurized stages. The aim of the study is to find out the biodiversity of α and β diversity of the microbiota in the level of the genus in dadiah. The V3 -V4 hypervariable region of the 16 S rRNA deep sequencing was used to the detected genus of bacteria. The available data were analyzed by Alpha Diversity (richness and evenness) and Beta Diversity (Taxonomy Chart Heatmaps). These studies revealed that the most divergent sample is appropriate to C3 with Shanon diversity Index 2.014; meanwhile, the most evenness fit in to B4 with the Simpson diversity index of 0.573. Further, the highest value of the heatmap taxonomic chart on the B4 sample has its place in Lactococcus. There were differences in dadiah microbiota composition among the samples based on different areas. Each sample was found Lactococcus and Lactobacillus with the largest populations.
Nutrient analysis of dadih from Lintau Regency, West Sumatra, Indonesia
Authors: Alzahra Hurriya , Melia Sri , Susmiati  
Published: November 2021

AbstractDadih is a traditional food from buffalo milk fermentation in West Sumatra, Indonesia. Which is fermented for 24-48 hours at room temperature.This study aimed to determine the chemical properties of Dadih from Lintau Regency, West Sumatra, Indonesia. The measured Variables were protein content, fat content, water content, and pH. The research method was a descriptive method and analysis in the laboratory. The sample used was taken in Lintau Regency, West Sumatra, Indonesia. The results showed that the nutrition composition of Dadih is: protein content ranged from 7.58-8.92%, fat content ranged from 5.60-6.18%, water content ranged from 72.43-74.86%, and pH ranged from 5.1-5.4. The conclution is that Dadih has good nutritional quality.
Physical, mechanical, and microstructure properties of whey edible films incorporated with Virgin Coconut Oil (VCO)
Authors: Juliyarsi I , Melia S , Novia D , Nabila S 
Published: November 2021

Abstract
The edible film is a thin layer derived from edible materials and formed on top of food components serving as an inhibitor of mass transfer (e.g., moisture, oxygen, fat, and dissolved substances), a carrier of foodstuffs, and an additive to improve food handling. Virgin Coconut Oil (VCO) contains lactic acid bacteria that are probiotics and anti-microbial, adding edible whey film to utilize cheese waste into biodegradable packaging. The research aims to study the effect of VCO on barrier, mechanical, and microstructure whey edible films. This research used whey, VCO, glycerol, and Carboxymethyl Cellulose (CMC). The treatment in this study was the addition of VCO namely A (0%), B (0.5%), C (1%), D (1.5%) and E (2%). The result of the research showed that whey edible films with the addition VCO affected (P<0.05) physical properties (thickness and solubility time) and no effect (P>0.05) on mechanical properties (tensile strength and elongation) and microstructure.
Probiotic effect of fermented milk from Pediococcus acidilactici BK01 in fecal wistar rat microflora
Authors: Melia S , Juliyarsi I , Kurnia Y F , Fitria N , Pratama Y E , Ramadhanti N 
Published: November 2021

Abstract
Microorganisms that provide health benefits to the host when consumed in the right proportions are called Probiotics. Certain lactic acid bacteria (LAB) have been considered probiotics with proven health benefits. These probiotics have been used extensively for human health. In this study, in vivo evaluation was conducted to determine the count of microflora in the fecal of Wistar rats, which were given fermented milk with probiotic Pediococcus acidilactici BK01. Pediococcus acidilactici BK01 is a LAB with probiotic use, isolated from Bekasam (fermented fish). This research was conducted in vivo on 24 male rats (Wistar Rat). This study contain in 4 treatment groups. Each group contains six male rats. The results showed a significant increase in the group of rats given fermented milk compared to the control group (without fermented milk). The probiotics of fermented milk have significantly reduced the count of E.coli and not on the total aerobic bacteria. The conclusion of this study, probiotic of fermented milk Pediococcus acidilactici BK01, can increase the total microflora of lactic acid bacteria and reduce the number of pathogenic bacteria.
The effect of additional turmeric flour in rations on the performance and egg quality of laying chicken isa brown strain age 70th weeks
Authors: Jaelani A , Abelina N , Samudera R , Rostini T , Masganti  , Setyowati A I 
Published: November 2021

Abstract
This research aims to study and analyze the effect of adding turmeric flour (Curcuma domestica Val) in the rations on the performance of 70 weeks old Isa Brown strain of laying hens. The research method used in this study was an experimental method with a completely randomized design (CRD) step with five treatments and four replications. The number of chickens used was as many as 40 laying hens. The treatments given to the sample were P0 = feed without giving turmeric flour (control), P1 = feed with 0.3% turmeric flour, P2 = feed with 0.5% turmeric flour, P3 = feed with 0.7% turmeric flour and treatment P4 = feed by giving turmeric flour 0,9%. The results showed that giving turmeric flour in the rations of the Isa Brown strain of laying hens affected chicken performance and egg quality. This research concludes that giving turmeric flour a percentage of 0.7% in Isa Brown’s 70-week old laying hens is the best for ration consumption, Hen Day Production, egg weight, feed conversion, and egg quality: Haught Unit, yolk egg color, yolk egg index, grading egg and egg mass. Based on the haugh unit value, all eggs produced are still in the AA grade category
Synthesis, extraction and idetification of meat bioactive peptides: a review
Authors: Susanto Edy , Fadlilah Anik , Fathul Amin Muhammad 
Published: November 2021

Abstract
The consumption of meat should consider the concept of functional food. The meat had a highquality protein and contain of bioactive peptide compounds. Amino acid was component of bioactive peptides compound. It joined by covalent bonds known as amide or peptide bonds. A lot of research was currently focused on the bioactive peptide compounds isolated from myofibril and sarcoplasmic proteins with the synthesis, extraction, and identification methods. This study used a systematic review to get the structure of amino acids that the source of bioactive components and the principle of synthesis, extraction and identification of bioactive peptide in the meat. This paper highlights were finding on the structure of amino acid in the meat. The proportion of amino acids was also different in each animal body location. The result identified that more than 170 peptides were released from the main structure of the myofibril (actin, myosin) and sarcoplasmic muscle proteins, and the synthesis, extraction and bioactive peptide identification in the meat as well as their potential use as functional food.
Effect of the inclusion of different sources of carbohydrate in the fattening ration based on Leucaena leaves on the intake, digestibility, and weight gain of Culled Bali Cows
Authors: Malelak G E M , Jelantik I G N , Benu I 
Published: November 2021

Abstract
An experiment with the objective of improving the utilization of leucaena leaves by culled Bali cows by the inclusion of different sources of carbohydrate was conducted following a completely randomized design using 16 thin cull Bali cows. Four diets were allotted to each animal at any period including La: the animal was offered a diet consisting of 80% dried leucaena leaves and 20% rice straw as a control, LaLb: added with pumpkin, LaJg: added with cornmeal, and LaLbJg: added with pumpkin and cornmeal. All supplemented diets were set to have similar energy and protein level. Variables measured included intake and digestibility of nutrients, rumen environments, and daily weight gain. Results showed that the inclusion of pumpkin and especially cornmeal significantly increased (P<0,05) dry matter and nutrient intake. DM and nutrient digestibility did not differ among treatments except for crude fiber. Rumen concentration of volatile fatty acids was significantly increased but rumen ammonia concentration was reduced with the addition of carbohydrate. Daily weight gain was significantly (P<0,05) improved and the inclusion of cornmeal produced the highest daily weight gain. It can be concluded that the intake and the utilization of leucaena leaves for weight gain by culled Bali cows was improved by the inclusion of carbohydrates with cornmeal exert a better effect over pumpkin.
Evaluation of various salt addition levels in the pellets on performance and health of the domestic rabbit urination system
Authors: Siburian I S , Diapari D , Retnani Y 
Published: November 2021

Abstract
This research aims to evaluate the various salt addition levels in the pellets on the performance and health of the domestic rabbit urination system. The completely randomized design method was used to determine the 5 levels of salt addition to the treatment, namely P1, P2, P3, P4, and P5 with 0%, 0,175%, 0,25%, 0,375%, and 0,50%. The sample consists of 20 male domestic rabbits aged 8 weeks with an average body weight of 897,05 ± 68,27g, which were kept in individual cages. The variables of these rabbits were analyzed to determine their performance and urinalysis using Anova (analysis of variance) and correlation analysis. The Anova results showed that salt addition levels in the pellets had a significant effect on FI, WI, FCR, UV, Nit, Pro (p<0,05), and insignificant on BWG, pH, Leu, Uro, Blo, Ket, Bil, Glu, and SG (p>0,05). The results further showed the correlation on several variables of performance and urinalysis. In conclusion, the optimal level of salt addition in the pellets was 0,25% because it give the lowest value of feed conversion ratio and does not adversely affect the health of the domestic rabbit urination system.
Effect of protease in diets containing winged bean seeds (Psophocarpus tetragonolobus) on growth performance of broiler chickens
Authors: Nurpaidah  , Hermana W , Ridla M 
Published: November 2021

Abstract
This study aims to determine the effect of adding protease enzyme in diets that contains different levels of winged bean seeds on the growth performance of broiler chickens. In this study, a total of 240 one-day-old chicks were randomly grouped within six treatments and four replicates in a 3×2 factorial arrangements. The first factor was the level of winged bean seeds at 0%, 2.5%, and 5%, while the second was the enzyme treatment with and without protease. The results showed that the additive protease interaction and level of winged bean seeds on the diet had a significant effect on the feed conversion ratio value (P<0.05). Meanwhile, on the feed inateke, final, and body weight gain had no significant effect (p>0.05). Furthermore, the protease treatment significantly decreased feed intake in 35-day old broilers. (P<0.05). Based on these results, the addition of protease enzyme at the levels has the potential to offset the effect of winged bean seeds on growth performance of broiler chickens.
In vitro nutrient digestibility, volatile fatty acids and gas production of fermented palm fronds combined with tithonia (Tithonia diversifolia) and elephant grass (Pennisetum Purpureum)
Authors: Pazla R , Jamarun N , Agustin F , Zain M , Arief  , Cahyani N O 
Published: November 2021

Abstract
This study aims to determine the effect of the addition of phosphorus minerals to palm fronds fermented with Phanerochaete chrysosporium mold on the digestibility of nutrient invitro and to evaluate the total gas production and concentration of volatile fatty acids in fermented palm fronds combined with elephant grass (Pennisetum Purpureum) and Tithonia (Tithonia diversifolia). This research was conducted with an experimental method through 2 experimental stages. Stage 1 consisted of 3 treatment doses of mineral P with 5 replications, namely: A = 1000 ppm, B = 1500 ppm and C = 2000 ppm. Stage 2 consisted of 4 combined treatments of fermented palm fronds (FPF) with elephant grass (EG) and tithonia (T with 4 replications, namely: A = 20% FPF + 80% (EG + T), B = 40% FPF + 60% (EG + T), C = 60% FPF + 40% (EG + T) and D = 80% FPF + 20% (EG + T). The ratio of EG and T is 4: 1. The parameters measured in stage 1 were the digestibility of dry matter, organic matter, crude protein, and crude fiber, while in stage 2 were gas production and total and partial VFA concentrations. The results of the research in stage 1 showed that the treatment had a significant effect (P <0.01) on the digestibility of dry matter, organic matter, crude protein, and crude fiber. Gas production and total and partial VFA concentrations at stage 2 also showed significant differences (P <0.01) between treatments. This study concludes that the addition of a P dose of 2000 ppm (C) in the fermentation process provides the highest nutrient digestibility value and the combination of 20% FPF and 80% (EG + T) (D) results in the highest gas production and VFA concentration.
Effect of addition cattle feed supplement on in vitro fermentation, synthesis of microbial biomass, and methane production of rice straw fermentation basal diets
Authors: Ramaiyulis  , Yulia E , Sari D K , Nilawati  
Published: November 2021

Abstract
The objective of this study was to evaluate the influence of supplementation of cattle feed supplement (CFS) and concentrate in ruminant diets based on rice straw fermented (R) on in vitro rumen fermentation, microbial biomass synthesis, and enteric methane production. Five experimental diets were evaluated, consist of R = rice straw fermented 100%, RS = R + CFS 10%, RSC1, 2 and 3 = RS + Concentrate levels 10, 20 and 30 (%DM). Supplementation of CFS increased the gas production (P < 0.05) and highest in treatments RSC1 and 2 (44.09 and 44.87 ml/g substrate, respectively) and was decreased proportions of methane by inhibition rate until 49.80%. Ruminal protozoa population increased by CFS dan concentrate supplementation (P<0,05) and was dominated (>80%) of Entodinium genus. The treatments RS dan RSC1 promoted greater (P < 0.01) microbial biomass synthesis (386.32 and 312.39 mg/g substrate, respectively). In conclusion, the supplementation of CFS and concentrate in ruminant diets based on rice straw fermented can promote a greater synthesis of microbial biomass and mitigation of methane production.
The effect of Indigofera zollingeriana supplementation to performance of rabbit
Authors: Haryati T , Soewandi Bayu P , Pratiwi N , Komarudin K 
Published: November 2021

AbstractRabbit has potency to produce meat since it has high growth rate, litter size, and short calving interval. Rabbit can utilize low nutritional feed and wide various of forages as its feed. Recently,Indigofera Zollingeriana(IZ) has been widely used as a green protein source in livestock. The used of IZ in rabbit feed seemed to be able to reduce production cost. The study had objective to observe the effect of IZ supplementation in feed to rabbit performance. A number of 72 heads of 12 weeks old of New Zealand white rabbits were used in the study. Rabbits were distributed in three groups of IZ supplementation levels (R1: 0 %; R2: 20 %, and R3: 30%). The supplementation was applicated for five weeks. Data were analysed using one- way ANOVA. The results showed that IZ supplementation effected body weight gain (BWG), feed conversion ratio, and dry matter and energy digestibility (P<0.05). The R2 was the optimal IZ supplementation in the study. The average BWGs of rabbits were 833.38 ± 232 g, 688.50 ± 88 g, and 485.63 ± 130 g for R1, R2, and R3, respectively. In conclusion, the IZ supplementation up to 20 % could be applicated in rabbit feed to reduce cost