bluuebunny/crossref_metadata_2025_split huggingface dataset:
Molecular sexual determinants in Pistacia genus by KASP assay
Authors: Sahin Zeyneb Nur , Sahin Ezgi Cabuk , Aydin Yildiz , Uncuoglu Ahu Altinkut 
Published: May 2021

Abstract
I. Background: Pistacia

is kind of unisexual plant species that can take 4–5 years to initiate bearing stage of trees in terms of economic value. The fruits of species have an extreme importance in food, health and baking industry as a raw material. So, identify the individual in early juvenile period for pollination and position of trees is very crucial for growers. The objective of this study is developing markers for each Pistacia species which helps the screening the sex of plant seedling quickly before they reach reproduction stage without waiting morphological data.
II. Methods and Results:

Within the context, by using the power of KASP (Kompetitive Allele Specific PCR) assay technology as a marker screening system, we successfully discriminate 7 Pistacia species out of 8 which are P. atlantica, P. integerrima, P. khinjuk, P. mutica, P. terebinthus, P. vera and P. lentiscus. We have used a high-throughput DNA sequence read archive (SRA) to assemble a reference genome to use in our studies as de novo bioinformatics method. 4 genomic regions were sequenced with collected plant material from Antepfıstıgı Research Institute Collection Garden predominantly and 8 species were aligned intraspecifically for Single Nucleotide Polymorphism (SNP) mining. 12 SNP markers are converted to KASP markers and 5 of them (SNP-PIS-133396, SNP-PIS-167992, P-ATL-91951-565, P-INT-91951-256, P-KHI-91951-115) showed obvious allelic discrimination between male and female species. SNP-PIS-167992 and P-ATL-91951-565 were identified as best marker assays due to the allelic frequency differences in graphs which are apparent for all individuals and homozygosis/heterozygosis observed clearly. These markers could be most comprehensive for whole genus because the discriminability power of several species in once.
III. Conclusions:

As a conclusion, this study is reported first Pistacia gender discrimination by KASP, besides the precursor study for sex discrimination by KASP in plants.
Circ-GALNT16 Restrains Colorectal Cancer Progression by Enhancing the SUMOylation of hnRNPK
Authors: Peng Chaofan , Tan Yuqian , Yang Peng , Jin Kangpeng , Zhang Chuan , Peng Wen , Wang lu , Zhou Jiahui , Chen Ranran , Wang Tuo , Jin Chi , Ji Jiangzhou , Feng Yifei , Tang Junwei , Sun Yueming 
Published: May 2021

Abstract
BackgroundEmerging studies have investigated circRNAs as significant regulation factors in multiple cancer progression. Nevertheless, the biological functions and underlying mechanisms of circRNAs in colorectal cancer progression remain unclear.MethodsA novel circRNA (circ-GALNT16) was identified by microarray and qRT-PCR. A series of phenotype experiments in vitro and vivo were performed to investigate the role of circ-GALNT16 in CRC. FISH, RNA pulldown assay, RIP assay, RNA sequencing, coimmunoprecipitation, and ChIP were constructed to explore the molecular mechanisms of circ-GALNT16 in colorectal cancer.ResultsCirc-GALNT16 was downregulated in colorectal cancer and negatively correlated with poor prognosis. Circ-GALNT16 suppressed the proliferation and metastasis ability of colorectal cancer in vitro and vivo. Mechanistically, circ-GALNT16 could bind to the KH3 domain of heterogeneous nuclear ribonucleoprotein K (hnRNPK), which resulted in the SUMOylation of hnRNPK. Additionally, circ-GALNT16 could enhance the hnRNPK-p53 complex by facilitating the SUMOylation of hnRNPK. Furthermore, RNA sequencing assay identified serpin family E member 1 as the target gene of circ-GALNT16 at the transcriptional level. Rescue assays revealed that circ-GALNT16 regulated the expression of Serpine1 by inhibiting the deSUMOylation of hnRNPK mediated by SUMO specific peptidase 2 and then regulating the sequence-specific DNA binding ability of the hnRNPK-p53 transcriptional complex.Conclusions﻿Circ-GALNT16 suppressed CRC progression via inhibiting Serpine1 expression through adjusting the sequence-specific DNA binding ability of the SENP2-mediated hnRNPK-p53 transcriptional complex and might work as a biomarker and therapeutic target for CRC.
Extra-Corporeal Membrane Oxygenation (ECMO) for Postcardiotomy Cardiogenic Shock (PCCS) in Asian Patients: A Systematic Review and Meta-Analysis
Authors: Li Di-huan , Sun Ming-wei , Li Ting , Yuan Ting , Zhou Ping , Zeng Jun , Wang Yu , Jiang Hua 
Published: May 2021

Abstract
Background Extra-corporeal membrane oxygenation (ECMO) is an important extracorporeal life support system to treat patients with postcardiotomy cardiogenic shock (PCCS). But its effectiveness and safety are still inconclusive. Existing systematic reviews and meta-analysis have heterogeneity challenges such as the inclusion of different races in the same study.Objective The impact of ECMO treatment on the survival rate of Asian adult patients with PCCS was evaluated by searching the literature and using the method of systematic review and meta-analysis.Study Design and Methods PubMed, Web of Science, Cochrane Library, EMBASE, CNKI, WANFANG MED ONLINE, SinoMed were searched to find relevant research on the use of ECMO on PCCS patients in Asia. Outcomes included survival rate to hospital discharge, long-term survival rate, complications.Results A total of 32 articles were selected, including observational studies and one RCT, involving China and Japan covering 4,278 PCCS patients. The pooled rate of survival to hospital discharge was 43.0% (95% CI 38% ~47%, I2 = 87%), the pooled 1-year, 2-year, and 3-year survival rate were 34% (95% CI 26% ~ 42%, I2 = 85%), 29% (95% CI 18% ~39%, I2 = 93%), 25% (95%CI 16% ~35%, I2=93%). The pooled rate of bleeding, neurologic complications, rethoracotomy, leg ischemia, renal failure, renal replacement therapy, and infection were 18% (95% CI 13% ~24%, I2=91%), 13% (95% CI 9% ~17%, I2 = 90.0%), 36% (95% CI 27% ~46%, I2 = 97.0%), 11% (95% CI 9% ~13%, I2 = 55%), 40% (95% CI 27% ~54%, I2 = 90%), 45% (95% CI 35% ~ 56%, I2 = 98%), 26% (95%CI (20%~31%, I2=95%).Conclusion ECMO can provide survival benefits for Asian adult patients with PCCS. However, attention should be paid to reducing the incidence of complications, especially renal failure. More high-quality clinical studies are needed to confirm the survival benefit of ECMO.
Atovaquone-HSA Nano-drugs Enhance the Efficacy of PD-1 Blockade Immunotherapy by Improving Hypoxic Tumor Microenvironment
Authors: Wang Simeng , Zhou Xinrui , Zeng Zekun , Sui Mengjun , Chen Lihong , Feng Chao , Huang Chen , Yang Qi , Ji Meiju , Hou Peng 
Published: May 2021

Abstract
Background: Hypoxia is inherent character of most solid malignancies, leading to the failure of chemotherapy, radiotherapy and immunotherapy. Atovaquone, an anti-malaria drug, can alleviate tumor hypoxia by inhibiting mitochondrial complex Ⅲ activity. The present study exploits atovaquone/albumin nanoparticles to improve bioavailability and tumor targeting of atovaquone, enhancing the efficacy of anti-PD-1 therapy by normalizing tumor hypoxia.Methods: We prepared atovaquone-loaded human serum albumin (HSA) nanoparticles stabilized by intramolecular disulfide bonds, termed HSA-ATO NPs. The average size and zeta potential of HSA-ATO NPs were measured by particle size analyzer. The morphology of HSA-ATO NPs was characterized by transmission electron microscope (TEM). The bioavailability and safety of HSA-ATO NPs were assessed by animal experiments. Immunofluorescence and ELISA assays were used to evaluate tumor immune microenvironment.Results: Our data first verified that atovaquone effectively alleviated tumor hypoxia by inhibiting mitochondrial activity both in vitro and in vivo, and successfully encapsulated atovaquone in vesicle with albumin, forming HSA-ATO NPs of approximately 164 nm in diameter. We then demonstrated that the HSA-ATO NPs possessed excellent bioavailability, tumor targeting and a highly favorable biosafety profile. When combined with anti-PD-1 antibody, we observed that HSA-ATO NPs strongly enhanced the response of mice bearing tumor xenografts to immunotherapy. Mechanistically, HSA-ATO NPs promoted intratumoral CD8+ T cell recruitment by improving tumor hypoxia microenvironment, thereby enhancing the efficacy of anti-PD-1 immunotherapy. Conclusion: Our data provide strong evidences showing that HSA-ATO NPs can serve as safe and effective nano-drugs to enhance cancer immunotherapy by improving hypoxic tumor microenvironment.
Low Dosage and Long-Term Use of Cyclophosphamide Improve the Survival of Patients with Systemic Lupus Erythematosus
Authors: Hussenbocus Yoosuf Ali Ashraf Muhammad , Jin Ziyi , Pan Wenyou , Liu Lin , Wu Min , Hu Huaixia , Ding Xiang , Wei Hua , Zou Yaohong , Qian Xian , Wang Meimei , Wu Jian , Tao Juan , Tan Jun , Da Zhanyun , Zhang Miaojia , Li Jing , Feng Xuebing , Sun Lingyun 
Published: May 2021

Abstract
Background: Cyclophosphamide (CTX) is an alkylating agent used in the treatment of systemic lupus erythematosus (SLE) for its potent immunosuppression effect. Many aspects of the use of CTX in SLE have been previously studied. However, its relation to mortality in SLE had rarely been mentioned. Thus we investigate the effect of cyclophosphamide on organ involvements and the overall and cause-specific mortality of SLE patients.Methods: Information about CTX prescription were taken from medical records in the Jiangsu Lupus database that was set up to collect data from SLE patients since their first admission during 1999-2009 in Jiangsu province, China. Follow- up studies were carried out in 2010 and 2015 to check the survival status of the patients. Cox regression models were used to estimate hazard ratio (HR) and 95% CI. Kaplan-Meier model was used to assess the effect of CTX on mortality between organ involvements and non-involvements.Results: There were 221 deaths observed out of 2446 SLE patients. CTX users showed a lower mortality of SLE (8.4%) with adjusted HR (95% CI) of 0.74 (0.56-0.97), as compared to non-users. A decreased in overall mortality of SLE in low daily dosage of CTX, with adjusted HR (95% CI) of 0.54 (0.36-0.81) and a significant dose-response pattern (P = 0.004) between overall mortality of SLE and long-term use of CTX with adjusted HR (95% CI) 0.53 (0.35-0.80) were observed. In cause-specific mortality analyses, protective effective of CTX was found to be insignificant. However, CTX could eliminate the differences in mortality between organ involvement and non-involvement, including renal, neuropsychiatric, cardiopulmonary, gastrointestinal and hematological involvements.Conclusion: Low daily dosage and long-term use of CTX lowers the risk of overall mortality of SLE. CTX might improve the survival of patients with renal, neuropsychiatric, cardiopulmonary, gastrointestinal and hematological involvements.
Human Cytomegalovirus mir-UL70-3p downregulates the H2O2 induced apoptosis in HEK293T cells by targeting the Modulator of Apoptosis-1 (MOAP1)
Authors: Pandeya Abhishek , Mishra Anup , Khalko Raj Kumar , Singh Sukhveer , Singh Nishant , Yadav Sanjay , Saha Sudipta , Saxena Sangeeta , Gosipatala Sunil Babu 
Published: May 2021

Abstract
Human Cytomegalovirus (HCMV), a prototypic member of the Beta-herpesvirinae subfamily, mainly responsible for congenital disabilities in newborns, cause opportunistic infections in immunocompromised individuals. Its seroprevalence varies across the globe ranging from 50–70 percent in developed countries to 90–100 percent in developing countries. Causing latent infections in the immunocompetent host suggests that it employs several strategies to escape the wrath of the host’s antiviral mechanisms. Apoptosis is an innate cellular response to viral infection, and downregulation of this mechanism by HCMV is a well-established phenomenon. HCMV utilizes its proteins, RNA and miRNA in regulating this response to establish a productive infection in the host. The role of HCMV miRNAs on cellular apoptosis is very interesting, where some miRNAs downregulate but a few upregulate this process. In the present study, we report the antiapoptotic activity of HCMV miRNA, miR-UL-70-3p, on H2O2 induced apoptosis in HEK293T cells. The ectopic expression of this HCMV miRNA in HEK293T cells downregulate the apoptosis, and continuing studies reveal that the proapoptotic gene, Modulator of Apoptosis Protein-1 (MOAP1), is a functional target for this miRNA. We verified the functionality of the binding site predicted in the 3'UTR of MOAP1 in our earlier studies through dual luciferase-based assays using both the wild and mutant 3'UTR’s of MOAP1. The MOAP1 protein levels were significantly downregulated by the miR-UL70-3p, suggesting that the MOAP1 mRNA was degraded after binding with the miR-UL70-3p. Further, the extent of MOAP1 mRNA and its protein inhibitions by HCMV miR-UL70-3p and siRNA of MOAP1 were compared and found that the siRNA of MOAP1 inhibits 69.52 percent of mRNA and 35.67 percent of MOAP1 protein; while the miR-UL70-3p inhibits 46.66 percent MOAP1 mRNA and 21.05 percent MOAP1 protein. Though the inhibitory activity of miR-UL70-3p is not equal to the siRNA of MOAP1, but it was significant enough in reversing the H2O2 induced apoptosis in HEK293T cells. These results confirm that hcmv-miR-UL70-3p downregulates H2O2 induced apoptosis in HEK293T cells by targeting the 3'UTR of MOAP1 mRNA.
Tumor microenviromental reaction would diminish incomparable accuracy and swiftness of flow cytometric diagnosis of primary central nervous system lymphoma
Authors: Takeuchi Hayato , Inaba Tohru , Shishido-Hara Yukiko , Tsukamoto Taku , Mizutani Shinsuke , Okamoto Takanari , Tanigawa Seisuke , Yamanaka Takumi , Takahashi Yoshinobu , Konishi Eiichi , Kuroda Junya , Hashimoto Naoya 
Published: May 2021

Abstract
Background

Primary central nervous system lymphoma (PCNSL), a relatively rare brain tumor, bears a dire prognosis. On occasion, rapid progression of the tumor makes immediate diagnosis and initiation of therapy imperative. To achieve swift diagnosis, we adopt flow cytometry (FCM) in addition to conventional histopathology. The aim of this study is to reveal utility and drawbacks of FCM diagnosis for PCNSL.
Methods

Patients with suspected PCNSL on neuroradiological findings and received both FCM and histological diagnosis between August 2015 and April 2020 were retrospectively enrolled into the study. Tumor samples were collected by craniotomy with either of endoscope or microscope. The patients’ electronic medical records were investigated, and histological findings, results of FCM, and other clinical data were evaluated.
Results

Twenty seven patients met the eligibility criteria. Twenty three patients (11 males and 12 females) were diagnosed with PCNSL by histological confirmation, and 22 cases were B-cell type lymphoma and 1 was T-cell type. Median age at diagnosis was 65. FCM analysis showed lymphoma pattern in 20 cases, but in the other 3 lymphoma cases (FCM discordant: FCM-D) and 4 non-lymphomatous tumor cases, FCM results did not show lymphoma pattern (sensitivity: 86.4%, specificity: 100%). Analysis of FCM-D cases showed infiltration of T lymphocytes or astrocytes into the tumor tissue, indicating tumor microenvironmental reaction, were observed, and it is assumed that those reactions deceived FCM diagnosis.
Conclusions

Despite some disadvantages, diagnosis of PCNSL by FCM is rapid and reliable.
The Complete Mitogenome of Curculio Chinensis (Coleoptera: Curculionidae: Curculioninae): Structural Characterization and Phylogenetic Implication
Authors: Yang Zaihua , Hu Kai , Zhang Niannian 
Published: May 2021

Abstract
To explore the phylogenetic position of Curculio chinensis Chevrolat, 1878 and phylogenetic relationships among major lineages of the family Curculionidae, we sequenced and annotated this mitogenome. The mitogenome is 18,680 bp in length, and includes the 37 typical mitochondrial genes and a large control region (length: 1,997 bp). Mitogenome organization, nucleotide composition, and codon usage are similar to most of the previously sequenced Curculioninae mitogenomes. All 13 protein-coding genes use ATN or TTG as start codon, and end with TAA/G or incomplete stop codons (single T-). Twenty-one transfer RNA genes have the typical clover-leaf structures, while the dihydrouridine (DHU) arm of trnS1 is missing. In Curculioninae mitogenomes, the size and number of tandem repeats in the control region are highly variable. Both ML and BI analyses based on the 13 PCGs and two rRNAs from 91 species of Coleoptera strongly supported the monophyly of Curculionidae and three of the included subfamilies (Platypodinae, Dryophthorinae, and Cryptorhynchinae) plus the sister relationship between Platypodinae and Dryophthorinae. Additionally, the monophyly of the genus Curculio was recovered with strong support.
A Novel Monoclonal Antibody to PL2L60 is Effective For Therapy of Various Types of Cancers in Human and Mice
Authors: Lu Hong-Min , Fu Yu-Jie , Liu Ning , Xia Wu-Yan , Chen Hai-Yan , Liu Meng-Yao , Li Lin-Feng , Gao Jian-Xin 
Published: May 2021

Abstract
Background: PL2L60 is a PIWIL2-like (PL2L) protein that is specifically and widely expressed in various types of hematopoietic and solid tumors. However, it is unknown whether PL2L60 is an effective target for cancer immunotherapy. The current study aimed to investigate the efficacy of an monoclonal antibody (mAb) to PL2L60 (clone KAO3；IgM isotype) in treatment of the cancers either from human or mouse.Methods: The expression of PL2L60 protein in the cell surface and cytoplasm were determined in a panel of human and mouse tumor cell lines by flow cytometry, immunofluorescent staining and Western Blotting. The apoptosis and the cell cycle arrest of the tumor cells treated with mAb KAO3 were evaluated by flow cytometry. The tumorigenesis of the mAb KAO3-pretreated tumor cells was determined by tumor incidence and tumor size, and efficacy of mAb KAO3 treatment on tumor growth in tumors-bearing mice were kinetically evaluated. Complement-dependent cytotoxicity tests were utilized to determine the mechanism of mAb KAO3 killing tumor cells. Results: Treatment of human or mouse tumor cells with mAb KAO3 at the time of inoculation efficiently inhibited their tumorigenesis in the immunodeficient mice. Moreover, injection of mAb KAO3 into established tumors significantly inhibited their growth, and prolonged survival of the tumor-bearing mice, including lymphoma, breast cancer, lung cancer and cervical cancer. The inhibitory effects of mAb KAO3 were likely associated with its binding to the PL2L60 expressed on tumor cell surface, which may induce cell apoptosis through either activation of complement or blocking cell cycling. The cell cycling was arrested at G2/M phase and DNA synthesis may be inhibited.Conclusion: We have identified the PL2L60 as a novel tumor-specific and broad-spectral biomarker, which is recognized by the mAb KAO3 as an efficient target for immunotherapy of both solid and hematopoietic cancers.
Big Data and Energy Security: Impacts on Private Companies, National Economies and Societies
Authors: Hassani Hossein , Komendantova Nadejda , Kroos Daniel , Unger Stephan , Yeganegi Mohammad Reza 
Published: May 2021

Abstract
The importance of energy security for successful functioning of private companies, national economies, and the overall society should not be underestimated. Energy is a critical infrastructure for any modern society, and its reliable functioning is essential for all economic sectors and for the well-being of everybody. Uncertainty in terms of the availability of information, reliable data to make predictions and to plan for investment as well as for other actions of stakeholders at the energy markets is one of the factors, which has the highest influence on energy security. This uncertainty can be connected with many factors such as the availability of reliable data or the actions of stakeholders themselves. For example, the recent outbreak of the COVID-19 pandemic revealed negative impacts of uncertainty on decision-making processes and markets. At the time point when the market participants started to receive real-time information about the situation, the energy markets began to ease. This is one scenario where Big Data can be used to amplify information to various stakeholders to prevent panic and to ensure market stability and security of supply. In a fast-paced digital world characterized by technological advances, the use of Big Data technology provides a unique niche point to close this gap in information disparity by levering the use of unconventional data sources to integrate technologies, stakeholders, and markets to promote energy security and market stability. The potential of Big Data technology is yet to be fully utilized. Big Data can handle large data set characterized by volume, variety, velocity, value, and complexity. The challenge for energy markets is to leverage this technology to mine available socioeconomic, political, geographic, and environmental data responsibly as well as to provide indicators that predict future global supply and demand. This information is crucial for energy security and ensuring global economic prosperity.
The characteristics and factors of the internalizing and externalizing behaviours of children at high risk for autism spectrum disorder
Authors: Ding Ning , Ren Yanling , Jiang Jiying , Zhai Mengyao , Shao Huan , Fu Linyan , Li Chunyan , Gao Huiyun , Li Yu , Feng Min , Cui Xiwen , Qiu Nana , Jin Peiying , Ke Xiaoyan 
Published: May 2021

Abstract
Background: Children with autism spectrum disorder (ASD) are at risk of developing internalizing and externalizing problems. However, information on the early development of behavioural problems and the contributing role of parent-child interactions among infants at high risk for ASD is lacking. We tested whether parent-child interactions and parenting characteristics were associated with behavioural problems during the infant and toddler periods and examined the effects of the intervention on behavioural problems.Methods: This study collected data from 91 infants at high for risk ASD and 68 matched typically developing (TD) infants. At baseline, behavioural observation was performed during free play with all the enrolled families, and in the ASD group, a second behavioural observation was performed after 12 weeks of the Play-based Communication and Behavior Intervention (PCBI). The coding of parent-child interactions was based on the behaviour of parents and children during free play. These data included the child’s engagement with each parent and behavioural problems.Results: During free play, infants in the ASD group showed more internalizing behaviours (p<0.001) and externalizing behaviours (p<0.05) and less positive engagement (p<0.01) than TD infants. After 12 weeks of intervention, we found that infants at high for risk ASD had reduced internalizing behaviours (p<0.001), increased parent positive engagement (p<0.001), and improved parent-child interactions (p<0.001). In the regression analysis, we found that parenting stress had an impact on infants’ externalizing behaviours (△R2 = 0.215). Parent negative engagement had an impact on infants’ internalizing behaviours (△R2 = 0.451).Conclusions: The findings on the contribution of both parent negative engagement and parent-child interactions to behavioural problems suggests that the amelioration of parent negative engagement is an important factor for the mitigation of behavioural problems. The amelioration of parenting stress is associated with externalizing behaviours. Early intervention and parental factors are important for preventing behavioural problems and possible comorbidity problems in the future.