bluuebunny/crossref_metadata_2025_split huggingface dataset:
SEMAPHORINS and their receptors: focus on the crosstalk between melanoma and hypoxia
Authors: Valentini Elisabetta , Di Martile Marta , Del Bufalo Donatella , D’Aguanno Simona 
Published: April 2021

AbstractHypoxia, a condition of oxygen deprivation, is considered a hallmark of tumor microenvironment regulating several pathways and promoting cancer progression and resistance to therapy. Semaphorins, a family of about 20 secreted, transmembrane and GPI-linked glycoproteins, and their cognate receptors (plexins and neuropilins) play a pivotal role in the crosstalk between cancer and stromal cells present in the tumor microenvironment. Many studies reported that some semaphorins are involved in the development of a permissive tumor niche, guiding cell-cell communication and, consequently, the development and progression, as well as the response to therapy, of different cancer histotypes, including melanoma.In this review we will summarize the state of art of semaphorins regulation by hypoxic condition in cancer with different origin. We will also describe evidence about the ability of semaphorins to affect the expression and activity of transcription factors activated by hypoxia, such as hypoxia-inducible factor-1. Finally, we will focus our attention on findings reporting the role of semaphorins in melanocytes transformation, melanoma progression and response to therapy. Further studies are necessary to understand the mechanisms through which semaphorins induce their effect and to shed light on the possibility to use semaphorins or their cognate receptors as prognostic markers and/or therapeutic targets in melanoma or other malignancies.
Resuscitative transesophageal echocardiography in the diagnosis of post-CABG loculated pericardial clot causing cardiac tamponade
Authors: Adi Osman , Ahmad Azma Haryaty , Fong Chan Pei , Ranga Asri , Panebianco Nova 
Published: April 2021

Abstract
Background
Pericardial effusion is a known complication of post-open cardiac surgery which can progress to life-threatening cardiac tamponade. Classical signs of tamponade such as hypotension and pulsus paradoxus are often absent. Diagnosing acute cardiac tamponade with transthoracic echocardiography (TTE) can be challenging in post-cardiac surgical patients due to distorted anatomy and limited scanning windows by the presence of surgical dressings or scar. Additionally, this patient population is more likely to have a loculated pericardial effusion, or an effusion that is isoechoic in appearance secondary to clotted blood. These findings can be challenging to visualize with traditional TTE. Missed diagnosis of cardiac tamponade due to loculated pericardial clot can result in delayed diagnosis and clinical management.

Case presentation
We report a case series that illustrates the diagnostic challenge and value of resuscitative transesophageal echocardiography (TEE) in the emergency department (ED) for the diagnosis of cardiac tamponade due to posterior loculated pericardial clot in post-surgical coronary artery bypass graft (CABG) patients.

Conclusions
Cardiac tamponade due to loculated posterior pericardial clot post-CABG requires prompt diagnosis and appropriate management to avoid the potential for hemodynamic instability. Transesophageal echocardiography allows a rapid diagnosis, early appropriate referral and an opportunity to institute appropriate therapeutic measures.
KLF7/VPS35 axis contributes to hepatocellular carcinoma progression through CCDC85C-activated β-catenin pathway
Authors: Guo Yarong , Chai Bao , Jia Junmei , Yang Mudan , Li Yanjun , Zhang Rui , Wang Shunmin , Xu Jun 
Published: April 2021

Abstract
Objective
Dysregulation of KLF7 participates in the development of various cancers, but it is unclear whether there is a link between HCC and aberrant expression of KLF7. The aim of this study was to investigate the role of KLF7 in proliferation and migration of hepatocellular carcinoma (HCC) cells.

Methods
CCK8, colony growth, transwell, cell cycle analysis and apoptosis detection were performed to explore the effect of KLF7, VPS35 and Ccdc85c on cell function in vitro. Xenografted tumor growth was used to assess in vivo role of KLF7. Chip-qPCR and luciferase reporter assays were applied to check whether KLF7 regulated VPS35 at transcriptional manner. Co-IP assay was performed to detect the interaction between VPS35 and Ccdc85c. Immunohistochemical staining and qRT-PCR analysis were performed in human HCC sampels to study the clinical significance of KLF7, VPS35 and β-catenin.

Results
Firstly, KLF7 was highly expressed in human HCC samples and correlated with patients’ differentiation and metastasis status. KLF7 overexpression contributed to cell proliferation and invasion of HCC cells in vitro and in vivo. KLF7 transcriptional activation of VPS35 was necessary for HCC tumor growth and metastasis. Further, co-IP studies revealed that VPS35 could interact with Ccdc85c in HCC cells. Rescue assay confirmed that overexpression of VPS35 and knockdown of Ccdc85c abolished the VPS35-medicated promotion effect on cell proliferation and invasion. Finally, KLF7/VPS35 axis regulated Ccdc85c, which involved in activation of β-catenin signaling pathway, confirmed using β-catenin inhibitor, GK974. Functional studies suggested that downregulation of Ccdc85c partly reversed the capacity of cell proliferation and invasion in HCC cells, which was regulated by VPS35 upregulation. Lastly, there was a positive correlation among KLF7, VPS35 and active-β-catenin in human HCC patients.

Conclusion
We demonstrated that KLF7/VPS35 axis promoted HCC cell progression by activating Ccdc85c-medicated β-catenin pathway. Targeting this signal axis might be a potential treatment strategy for HCC.
Inner ear salivary gland choristoma extending to the middle ear with congenital profound hearing loss and facial palsy: A case report
Authors: Yamanobe Yoshiharu , Oishi Naoki , Nishiyama Takanori , Hosoya Makoto , Ogawa Kaoru 
Published: April 2021

Background Salivary gland choristoma (SGCh) is a rare benign tumor reported in several unusual sites, such as the gastrointestinal tract, the optic nerve, and the internal auditory canal, but never reported in the inner ear. Case presentation An 8-year-old girl with a history of left profound congenital hearing loss presented to us with ipsilateral progressive severe facial nerve palsy (House-Brackmann Grade VI). The left tympanic membrane was swollen with a pulsatile tumor. Radiological investigations revealed a multilocular tumor in the inner ear extending into the middle ear and internal auditory canal (IAC). We performed a partial resection of the tumor by transmastoid approach to preserve the anatomical structure of the facial nerve. The tumor was pathologically diagnosed as SGCh. Two years after surgery, her facial function recovered to House-Brackmann Grade II and the residual tumor did not show regrowth on MRI. Conclusions Although the natural course of this rare tumor is unknown, a partial resection is an acceptable treatment procedure when functional recovery of the facial nerve is anticipated. Graphical abstract
Song divergence indicates an unclear relationship between the Neotropical and Nearctic Horned Larks
Authors: Vargas-Puentes Jimmy Alexander , Arias-Sosa Luis Alejandro , Ramos-Montaño Carolina 
Published: April 2021

AbstractBackgroundSome studies have indicated that the Horned Larks (Eremophila alpestris) should be considered as a species complex. Recently it split into four species or clades and seven subclades based on genetic, morphological, and biogeographic data. However, other aspects like song divergence have not been studied and several subspecies have not been evaluated, leading to important information gaps in this group of birds. In this work, we aimed to assess the differences in song traits and playback response between the Nearctic subclade and the Neotropical or Colombian subspeciesE. a. peregrina.MethodsWe compared six song traits between these groups and performed field playback experiments, to test the response of the Neotropical larks to both songs. We tested the difference in the variables for separate as well as by principal component analysis (PCA).ResultsWe found significant differences (p < 0.05) in the individual song traits and the PCA analysis between the two groups. Further, the PCA analysis showed a clearer divergence of the Neotropical songs in comparison to the Nearctic songs of different locations within North America. Similarly, the playback analysis showed a significantly lower response ofE. a. peregrinato the songs of the Nearctic larks.ConclusionsBesides this song divergence, there are important ecological and biogeographic differences between the Neotropical and Nearctic Horned Larks, that indicate an unclear relationship between these two groups. Thus, further morphological and genetic studies are required to clarify the taxonomy of the Neotropical Horned Lark and define if they share the same evolutionary history as the other subspecies of the Nearctic subclade.
Reversible acute Fontan circulation failure secondary to retrogradely conducted junctional rhythm: clinical echocardiographic correlation
Authors: Ferrero Paolo , Piazza Isabelle , Sadou Youcef , Ciuffreda Matteo 
Published: April 2021

Abstract
Background
Sequential atrioventricular activation plays a critical role in the physiology of Fontan circulation. Although bradycardia is usually well tolerated, retrogradely conducted junctional rhythm may acutely increase atrial pressure impairing cardiac output. Echocardiographic evaluation can reveal clues of this hemodynamic condition. The clinical impact of arrhythmic disturbance on the follow up of patients who had undergone total cavo-pulmonary connection is well recognized but the role of, transient periods of retrogradely conducted junctional rhythm on the immediate post-operative course is less defined.

Case presentation
We describe two cases of acute Fontan circulatory failure due to postoperative retrogradely conducted junctional escape rhythm despite an adequate heart rate and circadian variation. The patients rapidly improved after atrial pacing, allowing discharge with a minimal dose of diuretic.

Conclusion
In the absence of any hemodynamic target, hearth rhythm should be systematically checked after TCPC irrespective of adequacy of heart rate. Likewise, efficiency of temporary atrial pacing should be granted and surgeons should have a low threshold for epicardial lead implantation.
Machine learning model and strategy for fast and accurate detection of leaks in water supply network
Authors: Fan Xudong , Zhang Xijin , Yu Xiong ( Bill) 
Published: April 2021

AbstractThe water supply network (WSN) is subjected to leaks that compromise its service to the communities, which, however, is challenging to identify with conventional approaches before the consequences surface. This study developed Machine Learning (ML) models to detect leaks in the WDN. Water pressure data under leaking versus non-leaking conditions were generated with holistic WSN simulation code EPANET considering factors such as the fluctuating user demands, data noise, and the extent of leaks, etc. The results indicate that Artificial Neural Network (ANN), a supervised ML model, can accurately classify leaking versus non-leaking conditions; it, however, requires balanced dataset under both leaking and non-leaking conditions, which is difficult for a real WSN that mostly operate under normal service condition. Autoencoder neural network (AE), an unsupervised ML model, is further developed to detect leak with unbalanced data. The results show AE ML model achieved high accuracy when leaks occur in pipes inside the sensor monitoring area, while the accuracy is compromised otherwise. This observation will provide guidelines to deploy monitoring sensors to cover the desired monitoring area. A novel strategy is proposed based on multiple independent detection attempts to further increase the reliability of leak detection by the AE and is found to significantly reduce the probability of false alarm. The trained AE model and leak detection strategy is further tested on a testbed WSN and achieved promising results. The ML model and leak detection strategy can be readily deployed for in-service WSNs using data obtained with internet-of-things (IoTs) technologies such as smart meters.
Recurrent schwannoma of the tongue in a pediatric patient—report of a rare case with an updated review of literature
Authors: Singh Anup , Kuzhikkali Vaisakh , Kairo Arvind Kumar 
Published: April 2021

Abstract
Background
Head and neck is a relatively common site of occurrence for the peripheral nerve sheath tumors, and majority of these tumors are seen involving neck, involving vagus nerve, and cervical sympathetic chain. Schwannomas involving mobile tongue are rarely encountered, especially in the pediatric population

Case presentation
We present a case of recurrent tongue schwannoma in a 13-year-old female successfully managed with transoral excision. At a follow-up of 3 years, no recurrence is observed.

Conclusion
Surgical excision is the recommended modality of treatment for lingual schwannomas, and when excised adequately, recurrences are not expected. A clear margin of surrounding normal tissue should be aimed for to avoid possible recurrence.
Modified thoracoscopic pleural cryo-biopsy in diagnosis of exudative pleural effusion of undetermined etiology
Authors: Baess Ayman Ibrahim , Hassanein Essam Gouda , Emara Mohamed Al Saeed , Eshmawey Heba Ahmed 
Published: April 2021

Abstract
Background
Rigid thoracoscopy is the gold standard tool for diagnosing exudative pleural effusion, but sometimes it is difficult to obtain sufficient biopsies using the conventional forceps. This study evaluated the efficacy, safety, and diagnostic value of a modified technique using cryoprobe to obtain pleural biopsies during thoracoscopy.

Results
This is a single-center prospective study in patients that underwent rigid thoracoscopy using conventional rigid forceps followed by a cryoprobe at the same setting after injection of subpleural mixture of adrenaline and xylocaine. Biopsies were reviewed by an independent pathologist; any complications were recorded, and all patients were followed-up post-procedure. Twenty-four patients (12 males; mean age was 56 ± 15.1years) were included. The diagnostic yield of both rigid forceps biopsy (RFB) and cryoprobe biopsy (CPB) was 100%. CPB had more surface area than RFB with no difference regarding largest diameter (p = 0.064). RFB and CPB had similar results regarding presence of fat cells. CPB had much less crushed cells than RFB with better tissue preservation (p = 0.004). No significant complications were reported.

Conclusions
The modified cryoprobe biopsy is a highly effective and safe way for obtaining pleural biopsy besides affording good quality and size of the biopsy especially in thick firmly adherent pleura.
Sección de Medicina del Trabajo de la Unión Europea de Médicos Especialistas (UEMS)
Authors: Rodríguez-Jareño Maria Cruz 
Published: April 2021

El pasado día 5 de diciembre se celebró la única reunión realizada durante el 2020 por la sección de Medicina del Trabajo de la Unión Europea de Médicos Especialistas (UEMS)(1,2). Esta fue una reunión en circunstancias muy especiales por tener lugar en medio de la pandemia por COVID-19 que obligó a realizarla de forma telemática, después de haberse cancelado las reuniones previstas para la primavera y el otoño de 2020. A la reunión asistieron 22 representantes de 18 estados miembros.
Los estados representados fueron Alemania, Austria, Bélgica, Croacia, Dinamarca, Eslovenia, España, Finlandia, Hungría, Italia, Letonia, Luxemburgo, Noruega, Polonia, Portugal, Reino Unido, República Checa y Rumanía. También asistió la presidenta de EASOM (European Association of Schools of Occupational Medicine).
La presidenta de la Sección dio la bienvenida a los asistentes e informó de que todas las reuniones presenciales de todas las secciones de la UEMS habían sido canceladas debido a la pandemia por COVID-19…
Management of incontinence-associated dermatitis with topical antibiotics and antifungal medication
Authors: Wang Chin-Min , Chien Yu-Ju , Huang Chiao-Yi , Su Na-Chi , Lin Hsing Long , Chen Ching-Hsiang , Chen Chia-Jung , Hsu Honda 
Published: April 2021

Objective: The primary goals of managing incontinence-associated dermatitis (IAD) are to control the incontinence and to stop the progress of dermatitis. This study evaluated the effectiveness of using a combination of topical antibiotic and topical antifungal medication to manage IAD. Method: Patients with grade 2 IAD treated with a combination of topical antibiotic Biomycin (CBC Biotechnological and Pharmaceutical, Taiwan) and antifungal clotrimazole (Sinphar Group, Taiwan) between January 2017 and January 2019 were included in this retrospective study. Data collected included patients' age, sex, diagnosis, body mass index, comorbidities and surface area involved. Patients were reviewed fortnightly until the wounds had healed, the patient was discharged or had died. Results: A total of 76 patients were included. There were 39 men and 37 women with a mean age of 74 years. In 58 (76%) patients, the surface area involved was >50cm2, in 13 (17%) patients the involved area was 20–50cm2 and in five (7%) patients the area involved was <20cm2. The mean number of days treated was 10.3 (range: 1–53). A total of 46 (61%) patients showed total healing of their IAD, 17 (22%) patients showed improvement of >50% of the involved area, seven (9%) patients showed improvement of 0–50%, five (7%) patients showed no improvement and one (1%) patient showed an increase in the area involved. Conclusion: This combination of treatment was effective in the management of IAD. It was cheap, easy to apply, easy to remove and easily accessible. It could be used efficiently by the hospital staff and the patient's family.
Concurrent validity of biofilm detection by wound blotting on hard-to-heal wounds
Authors: Astrada Adam , Nakagami Gojiro , Minematsu Takeo , Goto Taichi , Kitamura Aya , Mugita Yuko , Sanada Hiromi 
Published: April 2021

Objective: Wound biofilms delay healing of hard-to-heal wounds. Convenient biofilm identification tools for clinical settings are currently not available, hindering biofilm-based wound management. Wound blotting with biofilm staining is a potential tool for biofilm detection, owing to its convenience. Although predictive validity of wound blotting has been established, it is necessary to confirm its concurrent validity. Furthermore, current staining systems employing ruthenium red have some disadvantages for clinical use. This study aimed to evaluate the usability of alcian blue as a substitute for ruthenium red. Method: Both in vitro and in vivo clinical samples were used to investigate validity and usability. Results: The in vitro study showed that proteins and extracellular DNA in biofilms did not affect staining ability of ruthenium red and alcian blue in the detection of biofilms. In the in vivo study, using a wound biofilm model with Pseudomonas aeruginosa, the staining sensitivity of ruthenium red was 88.9% and 100% for alcian blue, with correlation coefficients of signal intensities with native polyacrylamide gel electrophoresis (PAGE) of r=0.67 (p=0.035) and r=0.67 (p=0.036) for ruthenium red and alcian blue, respectively. Results from clinical samples were r=0.75 (p=0.001) for ruthenium red and r=0.77 (p<0.001) for alcian blue. The sensitivities of wound blotting staining by ruthenium red and alcian blue were very high and had a good correlation with native PAGE analysis. Conclusion: Because the alcian blue procedure is more convenient than the ruthenium red procedure, wound blotting with alcian blue staining would be a promising tool to guide clinicians in delivering biofilm-based wound management.
Reflection-type vapor cell for micro atomic clocks using local anodic bonding of 45° mirrors
Authors: Nishino Hitoshi , Yano Yuichiro , Hara Motoaki , Toda Masaya , Kajita Masatoshi , Ido Tetsuya , Ono Takahito 
Published: April 2021

This Letter reports the design, fabrication, and evaluation of reflection-type planar vapor cells for chip-scale atomic clocks. The cell with 2–8 mm cavity length contains two 45° Bragg reflector mirrors assembled using a local anodic bonding. Coherent population trapping resonance of Rb atoms is observed, realizing an atomic clock operation. Allan deviations at an averaging time of 1 s are

2.2

×

1

0

−
10

and

9.5

×

1

0

−
11

for 2 mm long and 6 mm long vapor cells, respectively. These results show that planar vapor cells compatible with a system-in-package are feasible without degradation of clock stabilities compared to conventional vertically stacked cells.
Intestinal endometriotic nodules with a length greater than 2.25 cm and affecting more than 27% of the circumference are more likely to undergo segmental resection, rather than linear nodulectomy
Authors: Abdalla-Ribeiro Helizabet , Maekawa Marina Miyuki , Lima Raquel Ferreira , de Nicola Ana Luisa Alencar , Rodrigues Francisco Cesar Martins , Ribeiro Paulo Ayroza 
Published: April 2021

Study objective
To analyze the efficacy of intestinal ultrasonography with bowel preparation (TVUSBP) for endometriosis mapping in evaluating intestinal endometriosis to choose the surgical technique (segmental resection or linear nodulectomy) for treatment.

Design
Cross-sectional observational study.

Setting
University Hospital—Center for Advanced Endoscopic Gynecologic Surgery from April 2010 to November 2014.

Patient(s)
One hundred and eleven women with clinically suspected endometriosis and intestinal endometriotic nodule or intestinal adherence in TVUSBP for endometriosis mapping.

Intervention(s)
All patients with suspected endometriosis underwent TVUSBP for endometriosis mapping prior to videolaparoscopy for complete excision of endometriosis foci, including intestinal foci, using the linear nodulectomy or segmental resection techniques, depending on the characteristics of the intestinal lesion with confirmation of endometriosis on anatomopathological examination.

Measurements and main results
Preoperative ultrasonographic assessment of the length of the intestinal nodule, circumference of the intestinal loop affected by the endometriotic lesion, distance from the anal verge and intestinal wall layers infiltrated by endometriosis, as well as other endometriosis sites. Of the 111 patients who participated in the study, 63 (56.7%) presented intestinal endometriotic nodules in ultrasonography, performed by a single examiner (A.L.A.N.), and underwent intestinal surgical treatment of deep endometriosis—linear nodulectomy or segmental resection. The analysis of the receiver operating characteristic (ROC) curve showed that a longitudinal length of the intestinal nodule of 2.25 cm and a loop circumference of 27% are cutoff points separating linear nodulectomy from segmental resection techniques for excising intestinal endometriosis. The information obtained by TVUSBP helps the surgeon and patient, in the preoperative period, to select the surgical technique to be performed for resection of intestinal endometriosis and plan the surgical procedure while taking into account postoperative morbidity.