bluuebunny/crossref_metadata_2025_split huggingface dataset:
Recurrent Uterine Tumor Resembling Ovarian Sex Cord Tumor: A Case report and Clinicopathological and Immunohistochemical Analysis
Authors: Wei Xiao-qiang , Tang Meng , Liu Lili , Zhao Shu-ping 
Published: July 2021

Abstract
Background: Uterine tumor resembling ovarian sex-cord tumor (UTROSCT) is a considerably rare gynecological tumor which has undetermined pathogenesis but with distinct polyphenotypic immunohistochemical expressions. According to the limited cases and follow-up information in the relevant literature, most of the tumors exhibit indolent or low malignant clinical course and the outcomes of the patients with the tumors generally have a good ending. But for the subset of UTROSCT with aggressive characters, the outcomes of the patients with recurrent neoplasm were not always satisfactory.Case presentation: In this case report, the recurrence of neoplasm was reported in the pelvic cavity after 53 months of surgery and irregular follow-up. The recurrent neoplasm grew in an invasive manner, and the arrangement of the recurrent neoplasm cells was closer, the nucleus atypia is more pronounced, and the cells demonstrated a more briskly mitotic activity (10 mitotic figures/10 HPF). The Ki67 index increased significantly and the expression of P53 was positive. For the recurrent tumor, both the clinical characteristics and the histological morphology of the recurrent neoplastic cells showed a more malignant behavior. The patient received a palliative resection of pelvic mass and bilateral oophorectomy, and she died of intestinal obstruction caused by the recurrent disease 9 months postoperatively.Conclusions: UTROSCT should be recognized as a definite malignant potential neoplasm. Based on the clinicopathological, immunohistochemistry parameters and the reviewed previous literature, we speculated that significant mitotic activity and large tumors (≥10 cm) were of value for the aggressive characters of the tumor, and the recurrence of the tumor might lead to a poor prognosis.
Endothelin B Receptor Mutant Exhibits Craniofacial Dysmorphology Resembling Domestication Syndrome
Authors: Chen Ko-Chin , Gee Allison , Croaker Geoffrey 
Published: July 2021

Abstract
Background: ETB-/- mutation is a major cause of HSCR, a neurocristopathy known for its enteric nervous system failure. Other than regulating ENCC migration, ETB mediates ET-1 clearance. Consequently, ETB may indirectly affect ET-1/ETA signaling, which controls CNCC migration and craniofacial development. Interestingly, it was hypothesized that “domestication syndrome” arise from changes in neural crest determining genes, including ETA and ETB. While ETA-/- animals are known to suffer severe dysmorphology resembling CATCH22 syndrome, we hypothesize that sl/sl rat, an ETB-/- HSCR model animal, may exhibit subtle craniofacial changes through indirect control. These features may share resemblance to those of domestication syndrome. Methods: Ten rat pups with an average age of 88 hours were anaesthetized with 5% isoflurane and culled via exsanguination. Tail tips were removed for genotyping. Head tissue were stained in 1.5% iodine for two weeks prior to micro-CT scanning. In vivo micro-CT scanning of cranial specimen was performed followed by ex vivo micro-CT scanning of 2 samples for image quality control. 3D visualization and analyses were performed using open-source program, Drishti. Cephalometric measurements were made based on selected craniofacial landmarks. Comparisons were made between sl/sl rats and the control group, which consisted of wild-type and heterozygotes. Results: Subtle reductions in facial measurements were seen in sl/sl rats when compared with the control group, ranging from 1.4% to 15%. These changes were observed in cranial, maxillary and mandibular parameters: total skull length, nasal length, nasal width, nasal cavity width, interorbital width, interlens distance, inner and outer canthal distance, maximal skull height, cranial length, intracranial length and width, interorbital width, and interzygomatic width. Consistently, craniofacial ratio indices showed sl/sl rat has a flatter cranium (skull height/skull length: 0.393 vs 0.413) and a shorter but broader nose (nasal-width/nasal-length: 0.794 vs 0.874). Additionally, subtle dystopia canthorum may be presented in sl/sl rat based on increased W index. While there was no discrepancy in dental number and morphology between the control and sl/sl groups, dimensional difference was detected. Conclusions: This study demonstrated subtle craniofacial changes are presented in ETB-/- HSCR model, supporting the idea that ETB regulates CNCC migration. The findings also implicate HSCR patient may have predisposing risks for conditions such as obstructive sleep apnea, cleft palate, or dental malocclusion. Lastly, these changes share resemblance with described domestication syndrome, supporting NCC-determining gene, ETB, may play a role in the formation of domestication.
TK/gI/gE/11K Genes Is Not Involved in the Virulence Enhancement of Highly Virulent Pseudorabies Virus
Authors: Tan Feifei , Guo Linghua , Zhang Chaoling , Yan Shijun , Wang Yuzhou , Xiao Yan , Wang Tongyan , Huang Baicheng , Tian Kegong 
Published: July 2021

Abstract
The large-scale outbreaks of Pseudorabies in china since 2011 in vaccine-immunized pig farms were caused by the highly virulent Pseudorabies virus (PRV). To investigate the factors involved in the virulence enhancement of the variant PRV, four recombinant viruses with the virulence gene’s replacement, gI/gE/11K and TK/gI/gE/11K, between the variant strain HN1201 and the classical strain Fa were constructed based on bacterial artificial chromosome infectious clones. Compared with their parental strain, the viral titers of the recombinant PRV strains are not strongly influenced by the replacement of TK/gI/gE/11K, while as previously reported, the strain HN1201 and its derived viruses showed the higher mortality, the severer clinical symptoms and tissues damage than that of strain Fa and its derived strains. In summary, the TK/gI/gE/11K genes of variant strain HN1201 showed no contribute to its virulence enhancement compares with the classical strain.
Features of Cerebral Demyelination and Clinical Relevance in Systemic Lupus Erythematosus
Authors: Guo Qian , He Yang , Liu Xia , Gao Xu guang , Xu Jing , Li Xue , Sun Yue , Xiang Yajuan , Li Ru , Li Zhanguo 
Published: July 2021

Abstract
We aimed to explore the clinical features and cerebral magnetic resonance imaging (MRI) changes of central nervous system demyelination in systemic lupus erythematosus (SLE) patients. Based on a consecutive cohort of 1191 SLE patients, 273 patients with cerebral MRI were enrolled to assess cerebral demyelination. Patients were divided into two groups, ie. complicated with or without demyelination. The MRI findings of the cerebral demyelination were divided into three categories: type A, periventricular white matter (WM) lesions; type B, subcortical WM lesions; type C, multiple discrete WM lesions. Among the 273 SLE patients with cerebral MRI, 35.9% (98/273) had demyelinating changes. The incidences of type A, B and C were 54.1% (53/98), 11.2% (11/98) and 92.9% (91/98), respectively. Fifty-one percent of the patients were with overlap of 2 or 3 types. Type C was the most common subgroup combined with other types. Compared with those without demyelination, the patients with demyelination were more likely to develop neuropsychiatric systemic lupus erythematosus (NPSLE), lupus nephritis (LN), hypertension and hyperuricemia (p < 0.05). Significantly higher rates of polyserous effusions and cardiac involvement were found in the patients with demyelination (p < 0.05). In addition, the patients with demyelination had higher frequency of proteinuria and higher levels of CD8+ T cells (p < 0.05). In multivariate logistic analysis, hyperuricemia and higher CD8+ T cells were significantly correlated with demyelination in SLE patients (p < 0.05). The data suggest that demyelination is a common complication in SLE patients and strongly associated with systemic involvements, including NPSLE, LN, polyserous effusions and cardiac involvement. Hyperuricemia and higher CD8+ T cells were independent risk factors for demyelination in SLE.
QAEmap: A Novel Local Quality Assessment Method for Protein Crystal Structures Using Machine Learning
Authors: Miyaguchi Ikuko , Sato Miwa , Kashima Akiko , Nakagawa Hiroyuki , Kokabu Yuichi , Ma Biao , Matsumoto Shigeyuki , Tokuhisa Atsushi , Ohta Masateru , Ikeguchi Mitsunori 
Published: July 2021

Abstract
Low-resolution electron density maps can pose a major obstacle in the determination and use of protein structures. Herein, we describe a novel method, quality assessment based on an electron density map (QAEmap), that evaluates local protein structures determined by X-ray crystallography and corrects structural errors using low-resolution maps. QAEmap uses a three-dimensional deep convolutional neural network with electron density maps and their corresponding coordinates as input and predicts the correlation between the local structure and the putative high-resolution experimental electron density map. This estimates how well the structure fits the high-resolution map. Further, we propose that this method may be applied to evaluate ligand binding, which can be difficult to determine at low resolution.
Methyloradius Palustris Gen. Nov., Sp. Nov., a Methanol-oxidizing Bacterium Isolated From Snow
Authors: Miyadera Takeshi , Kojima Hisaya , Fukui Manabu 
Published: July 2021

Abstract
A novel methylotrophic bacterium, strain Zm11 T , was isolated from reddish brown snow collected in a moor in Japan. Cells of the isolate were Gram-stain-negative, motile and rod-shaped (0.6-0.7×1.2-2.7 μm). Growth was observed at 5–32°C with an optimum growth temperature of 25–28°C. The pH range for growth was 5.4–7.8 with an optimum pH of 6.8. The strain utilized only methanol as carbon and energy sources for aerobic growth. The major cellular fatty acids (>40% of total) were summed feature 3 (C 16:1 ω 7 c and/or C 16:1 ω 6 c ) and C 16 : 0 . The predominant quinone was Q-8. The complete genome of strain Zm11 T is composed of a circular chromosome (2,800,413 bp), with G + C content of 46.4 mol%. Phylogenetic analyses were conducted based on the 16S rRNA gene sequence and conserved proteins encoded in the genome. The results of analyses indicate that strain Zm11 T is a member of the family Methylophilaceae but does not belong to any existing genus. On the basis of its genomic and phenotypic properties, strain Zm11 T (= DSM111909 T = NBRC114766 T ) is proposed as the type strain of a new species in a new genus, Methyloradius palustris gen. nov., sp. nov.
Spatiotemporal Change Analysis of Long Time Series Inland Water in Sri Lanka Based on Remote Sensing Cloud Computing
Authors: Li Jianfeng , Wang Jiawei , Yang Liangyan , Ye Huping 
Published: July 2021

Abstract
Sri Lanka is an important hub connecting Asia-Africa-Europe maritime routes, with abundant rainfall but uneven spatiotemporal distribution, and obvious seasonal water shortage. Monitoring water area change in its inland lakes and reservoirs plays an important role in guiding the development and utilization of water resources. In this study, a rapid extraction model of surface water based on Google Earth Engine remote sensing cloud computing platform was constructed. On the basis of evaluating the optimal spectral water index method, the spatiotemporal variation of typical reservoir and inland lakes and reservoirs in Sri Lanka were analyzed. The results showed that AWEIsh can accurately identify the water boundary, with an overall accuracy of 99.14%, which was suitable for the extraction of surface water in Sri Lanka. The area of Maduru Oya Reservoir showed an overall increasing trend on the basis of small fluctuations from 1988 to 2018, and the monthly area of reservoir fluctuated greatly in 2017, thus water resources management in dry zone should pay more attention to seasonal regulation and control. From 1995 to 2015, the number and area of lakes and reservoirs in Sri Lanka increased in different degrees, mainly concentrated in arid provinces such as Northern Province, North Central Province, Western Province, etc., and the amount of surface water resources increased as a whole.
Specific expression of lactate dehydrogenases in glioblastoma controls intercellular lactate transfer to promote tumor growth and invasion
Authors: Guyon Joris , Fernandez-Moncada Ignacio , Larrieu Claire , Bouchez Cyrielle , Chouleur Tiffanie , Espedal Heidi , Røsland Gro , Daher Boutaina , Barre Aurelien , Dartigues Benjamin , Coffe Aurelien , Rudewicz Justine , Romero-Garmendia Irati , Klink Barbara , Grützmann Konrad , Derieppe Marie-Alix , Molinié Thibaut , Obad Nina , Leon Céline , Miletic Hrvoje , Marsicano Giovanni , Nikolski Macha , Bjerkvig Rolf , Bikfalvi Andreas , DAUBON Thomas 
Published: July 2021

Abstract
Lactate is a central metabolite in brain physiology, involved in the astrocyte-neuron lactate shuttle, but also contributes to tumor development. Glioblastoma (GBM) is the most common and malignant primary brain tumor in adults, recognized by angiogenic and invasive growth, in addition to its altered metabolism. By adapting their glycolytic or oxidative metabolism, GBM stem-like cells are able to resist chemo- and radiotherapy. We show herein that lactate fuels GBM anaplerosis by replenishing the TCA cycle in absence of glucose. Lactate dehydrogenases (LDH) catalyze the interconversion of pyruvate and lactate. Deletion of either LDHA or LDHB did not alter significantly GBM growth and invasion. However, ablation of both LDH isoforms led to a reduction of tumor growth, and, consequently, to an increase in mouse survival. Comparative transcriptomics and metabolomics revealed metabolic rewiring involving high oxidative phosphorylation (OxPhos) in the double LDHA/B KO group which sensitized tumors to cranial irradiation, massively improving mouse survival. Survival was also increased when control mice were treated with the antiangiogenic treatment, bevacizumab, and the antiepileptic drug, stiripentol which targets LDH activity. Taken together, this highlights the complex metabolic network in which both LDH A and B are integrated and underscores that combined inhibition of LDHA and B is necessary to impact tumor development. Targeting of these enzymes in combination with anti-angiogenic and repurposed drugs may be of therapeutic benefit, especially when associated with radiotherapy.
Bioimpedance Measurement of Knee Injuries using Bipolar Electrode Configuration
Authors: Ye Xuanjie , Wu Lexi , Mao Kaining , Feng Yiwei , Lin Xianfeng , Chen Jie 
Published: July 2021

Abstract
Currently, there is no suitable solution for the point-of-care of knee injuries. A potential portable and low-cost technique for accessing and monitoring knee injuries is bioimpedance measurement. This study validated the feasibility of the bipolar electrode configuration for knee bioimpedance measurements with two electrodes placed on a fixed pair of knee acupuncture locations called Xiyan. Then, the study collected 82 valid samples to investigate the relationship between bioimpedance and knee injuries, among whom 45 patients, each with one healthy knee and one injured knee, and 37 individuals all with healthy knees. The self-contrast results indicated that knee injuries caused a reduction of bioimpedance of the knee by about 5% on average, which was detectable at around 100 kHz (p ≈ 0.001). Furthermore, the results analyzed by principal component analysis and support vector machines show that the detection sensitivity can reach 91.11% using the leave-one-out cross-validation.
Cauda equina syndrome caused by multiple intraspinal hemorrhage following percutaneous coronary intervention: a case report and a review of the literature
Authors: Lim Yong-Kuan , Chen Pin-Yuan , Chen Wei-Siang , Kao Yu-Cheng , Lin Mei-Mei , Zheng Yun-Cong 
Published: July 2021

Abstract
Background

Percutaneous coronary intervention and dual antiplatelet therapy are common management for patients with coronary artery disease. Multiple spontaneous intraspinal hematomas mixed with epidural hematoma and subdural hematoma following regular percutaneous coronary intervention is an extremely rare complication. We describe our experiences to treat the elderly who presented with spontaneous spinal epidural hematoma and subdural hematoma in different spinal locations after percutaneous coronary intervention. Neurological examination and magnetic resonance imaging were followed to assess the treatment outcomes for more than 2.5 years.
Case presentation:

In this article, we present a 70 years-old male taking dual antiplatelet therapy for 1 year after drug-eluting stents implantation to right coronary artery and left anterior descending artery for non-ST elevation myocardial infarction had a sudden onset of paraplegia then the autonomic dysfunction immediately after another percutaneous coronary intervention. Whole spinal MRI showed mixed spontaneous spinal subdural hematoma and spinal subdural hematoma, included: anterior C5-T3 acute subdural hemorrhage, right lateral T4 to T8 epidural hemorrhage, and L5-S1 intrathecal hematoma. After urgent cauda equina neurolysis and T7-9 laminectomy to evacuate spinal epidural hemorrhage in accordance with the neurological symptoms, the patient regained walking ability immediately. The urination and defecation function recovered then. The surgical results maintained for at less 2.5 years, even after another percutaneous coronary intervention 1 year later.
Conclusions

Intraspinal hematomas in acute coronary syndromes are scarce but critical conditions after percutaneous coronary intervention. Multiple mixed spontaneous spinal subdural hematoma/ epidural hematoma could be fully reversed by circumspect neurolysis and limited laminectomy timely.
Extending Theoretical Understanding for Defining the Capabilities of Big Data Analytics in Organisations: a Thematic Analysis
Authors: Sabharwal Renu , Miah Shah Jahan 
Published: July 2021

Abstract
Big Data Analytics (BDA) have been proliferated to academic researchers and industry practitioners over the past few years. As a prominent data-driven decision application, the BDA capabilities in organisation have been recognised, but limited studies have successfully attempted to communicate an authentic understanding on BDA capabilities that may enhance the current theoretical knowledge. While big data have been defined in various ways with its characteristics of shared definitions, it is important to explore the classification of BDA and its capabilities considering its advantageous opportunities. This study conducts a review study adopting the well-known PRISMA methodology, integrating a thematic analysis approach using NVIVO12. The study analyses 70 elected sample articles for generating new insights of BDA, informing through organisation development theory and leading to this an empirical research model is outlined for further validity assessment. It is anticipated that the findings would be contributing to address dynamic clarity and relevance of adopting BDA application.
Radiographic Assessment of Distal Radius Fracture Treatment Outcomes Following Various Types of Fracture Stabilization in Elderly Patients
Authors: Olech Jarosław , Kopczyński Bartosz , Morasiewicz Piotr 
Published: July 2021

Abstract
Background: Distal radius fractures pose a serious problem due to their high incidence and can be treated with various methods. No specific distal radius fracture treatment is acknowledged to be the gold standard by orthopedic surgeons. The aim of study was to conduct a comprehensive radiographic assessment of treatment outcomes in patients with distal radius fractures following various types of stabilization in elderly patients. Methods: We retrospectively assessed 122patients who underwent treatment for distal radial fracture in the years 2017and2018.There were three study groups: closed reduction with K-wire fixation (37patients),open reduction with volar plate fixation (42 patients),and closed reduction with cast immobilization (43patients).The mean age at the beginning of treatment was 73years.The mean follow-up period was 2 years and 7months.The following radiological parameters were evaluated: union rate, time to union, time of fracture immobilization, fracture stabilization failure, and the development of adjacent-joint arthritis. Results: There were no significant differences between the study groups in terms of union rate, time to union, and the development of adjacent-joint arthritis. After treatment, the lowest rates of posttraumatic intercarpal and carpometacarpal arthritis and the lowest rates of fracture stabilization failure in our study were observed in the plaster-cast group. Volar plate fixation was associated with the shortest duration of fracture stabilization. In comparison to the status from before treatment, all study groups showed increased rates of intercarpal and carpometacarpal arthritis after treatment. Conclusions: Our radiographic assessments demonstrated similar outcomes, regardless of the stabilization method. In treating distal radius fractures, we achieved good radiographic treatment outcomes irrespective of the fracture stabilization method used.
Predicting traffic flow evolution on degraded road networks
Authors: Shen Z. 
Published: July 2021

Abstract
In order to investigate the influence of the forecast information provided by ATIS on the traffic flow evolution of the degraded road network, a weighted moving average based travel time forecasting method is designed, and the path update rule is established in the forecast information environment. The proposed route update rule is used to analyze the effects of prediction information dependency parameters, information quality parameters, weight parameters and network degradation degree on traffic flow evolution for a small test network. The results show that: 1) there is a threshold value for the dependence of travelers on the predicted information in the information environment, when the dependence is less than this value, the traffic flow quickly evolves to a stable state, and when the dependence is greater than this value, the network traffic flow will oscillate; 2) there is a situation where the higher the quality of the predicted information provided by the information system, the worse the traffic flow evolution; 3) for a particular degraded network, there is a certain optimal combination of weights that can be used to guide the release of forecast information of the network; 4) the smaller the degradation of the road network, the greater the role played by the forecast information.
Intralocus conflicts associated with a supergene
Authors: Giraldo-Deck Lina , Loveland Jasmine , Goymann Wolfgang , Tschirren Barbara , Burke Terry , Kempenaers Bart , Lank David , Küpper Clemens 
Published: July 2021

Abstract
Chromosomal inversions frequently underlie major phenotypic variation maintained by divergent selection within and between sexes. Here we examine whether and how intralocus conflicts contribute to balancing selection stabilizing an autosomal inversion polymorphism in the ruff Calidris pugnax. In this lekking shorebird, three male mating morphs (Independents, Satellites and Faeders) are associated with an inversion-based supergene. We show that in a captive population, Faeder females, who are smaller and whose inversion haplotype has not undergone recombination, have lower average reproductive success in terms of laying rate, egg size and offspring survival than Independent females, who lack the inversion. Satellite females, who carry a recombined inversion haplotype and have intermediate body size, more closely resemble Independent than Faeder females in reproductive performance. We inferred that the lower reproductive output of Faeder females is primarily balanced by higher than average reproductive success of individual Faeder males, driven by negative frequency-dependent selection. These findings suggest that intralocus conflicts may play a major role in the evolution and maintenance of supergene variants.