bluuebunny/crossref_metadata_2025_split huggingface dataset:
Is positron emission tomography enough to rule out cardiac sarcoidosis? A case report
Authors: Huang Siyi , Kunchakarra Siri , Rathod Ankit 
Published: September 2021

Abstract

Background
Cardiac sarcoidosis (CS) is associated with poor prognosis, yet the clinical diagnosis is often challenging. Advanced cardiac imaging including cardiac magnetic resonance (CMR) and positron emission tomographic (PET) have emerged as useful modalities to diagnose CS.

Case summary
A 66-year-old woman presented with palpitations. A 24-h Holter monitor detected a high premature ventricular contraction burden of 25.6%. She underwent two transthoracic echocardiograms; both showed normal results. Stress perfusion CMR did not show any evidence of ischaemic aetiology; however, myocardial lesions detected by late gadolinium enhancement (LGE) imaging raised suspicion for CS. While there was no myocardial uptake of fluorodeoxyglucose (FDG) in subsequent cardiac PET, high FDG uptake was seen in hilar lymph nodes. Lymph node biopsy confirmed the diagnosis of sarcoidosis.

Discussion
Cardiac magnetic resonance and PET imaging are designed to evaluate different aspects CS pathophysiology. The characteristic LGE in the absence of increased FDG uptake suggested inactive CS with residual myocardial scarring.
Maternal hypercholesterolaemia during pregnancy affects severity of myocardial infarction in young adults
Authors: Cacciatore Francesco , Bruzzese Giuseppe , Abete Pasquale , Russo Giuseppe , Palinski Wulf , Napoli Claudio 
Published: September 2021

Abstract

Aims
Elevated maternal cholesterol during pregnancy (MCP) enhances atherogenesis in childhood, but its possible impact on acute myocardial infarction (AMI) in adults is unknown.

Methods and results
We retrospectively evaluated 310 patients who were admitted to hospital and whose MCP data were retrievable. Eighty-nine AMI patients with typical chest pain, transmural infarction Q-waves, elevated creatinine kinase, and 221 controls hospitalized for other reasons were identified. The AMI cohort was classified by MI severity (severe = involving three arteries, left ventricle ejection fraction ≤35, CK-peak &gt;1200 mg/dL, or CK-MB &gt;200 mg/dL). The association of MCP with AMI severity was tested by linear and multiple regression analysis that included conventional cardiovascular risk factors, gender, age, and treatment. Associations of MCP with body mass index (BMI) in patients were assessed by linear correlation. In the AMI cohort, MCP correlated with four measures of AMI severity: number of vessels (β = 0.382, P = 0.001), ejection fraction (β = −0.315, P = 0.003), CK (β = 0.260, P = 0.014), and CK-MB (β = 0.334, P = 0.001), as well as survival time (β = −0.252, P = 0.031). In multivariate analysis of patients stratified by AMI severity, MCP predicted AMI severity independently of age, gender, BMI, and CHD risk factors (odds ratio = 1.382, 95% confidence interval 1.046–1.825; P = 0.023). Survival was affected mainly by AMI severity.

Conclusions
Maternal cholesterol during pregnancy is associated with adult BMI, atherosclerosis-related risk, and severity of AMI.
Integrated analyses of SNP-genotype and environmental data in a continuously distributed snapper species (Lutjanus johnii, Bloch, 1792) reveals a mosaic of populations and a challenge for sustainable management
Authors: Taillebois Laura , Davenport Danielle , Barton Diane P , Crook David A , Saunders Thor , Hearnden Mark , Saunders Richard J , Newman Stephen J , Travers Michael J , Dudgeon Christine L , Maher Safia L , Ovenden Jennifer R 
Published: September 2021

AbstractUnderstanding population structure is important for predicting the outcomes of anthropogenic development, harvest, and environmental change on the abundance of fisheries species. Logically, the use of several data sources is best for delineating population structure, but combining across data types adds extra challenges as results can be incongruent. Here, genetic and environmental markers were used to test hypotheses about population structure in a collection of 523 tropical inshore finfish (Lutjanus johnii) across northern Australia from 20 locations. Redundancy analyses (RDA) were used to statistically test for correlations among data sources providing a basis for reconciliation and insights into ecological and evolutionary processes. Genomic data (10349 SNP loci) revealed low, but significant genetic divergence (max FST 0.0402) between 16 locations. A total of 12 putative contiguous biological stocks were proposed whose distributions were correlated with IMCRA bioregions but not to existing regions used for management of the fishery. RDA showed a significant correlation between environmental markers (otolith chemistry and parasite abundances) and SNP genotype in two of three region-wide analyses supporting the proposed stock structure, and suggesting that L. johnii may be locally adapted to the heterogeneous environment. These findings are significant for the conservation of the commercially and recreationally important L. johnii and can be used to guide future fisheries management actions.
Comparison of MICs in Escherichia coli isolates from human health surveillance with MICs obtained for the same isolates by broth microdilution
Authors: Suwono Beneditta , Hammerl Jens André , Eckmanns Tim , Merle Roswitha , Eigner Ulrich , Lümen Michaela , Lauter Sven , Stock Rüdiger , Fenner Ines , Boemke Eva , Tenhagen Bernd-Alois 
Published: September 2021

Abstract

Objectives
Human health surveillance and food safety monitoring systems use different antimicrobial susceptibility testing (AST) methods. In this study, we compared the MICs of Escherichia coli isolates provided by these methods.

Methods
E. coli isolates (n = 120) from human urine samples and their MICs were collected from six medical laboratories that used automated AST methods based on bacterial growth kinetic analyses. These isolates were retested using broth microdilution, which is used by the food safety monitoring system. The essential and categorical agreements (EA and CA), very major errors (VME), major errors (ME) and minor errors (mE) for these two methods were calculated for 11 antibiotics using broth microdilution as a reference. For statistical analysis, clinical breakpoints provided by EUCAST were used.

Results
Five study laboratories used VITEK®2 and one MicroScan (Walkaway Combo Panel). Out of 120 isolates, 118 isolates (98.3%) were confirmed as E. coli. The 99 E. coli isolates from five study laboratories that used VITEK®2 showed high proportions of EA and CA with full agreements for gentamicin, meropenem, imipenem and ertapenem. Additionally, 100% CA was also observed in cefepime. Few VME (0.5%), ME (1.9%) and mE (1.5%) were observed across all antibiotics. One VME for ceftazidime (7.1%) and 12 MEs for ampicillin (29.4%), cefotaxime (2.4%), ciprofloxacin (3.2%), tigecycline (1.5%) and trimethoprim (22.2%) were detected.

Conclusions
MICs from E. coli isolates produced by VITEK®2 were similar to those determined by broth microdilution. These results will be valuable for comparative analyses of resistance data from human health surveillance and food safety monitoring systems.
Predictions for anisotropic X-ray signatures in the circumgalactic medium: imprints of supermassive black hole driven outflows
Authors: Truong Nhut , Pillepich Annalisa , Nelson Dylan , Werner Norbert , Hernquist Lars 
Published: September 2021

ABSTRACT
The circumgalactic medium (CGM) encodes signatures of the galaxy-formation process, including the interaction of galactic outflows driven by stellar and supermassive black hole (SMBH) feedback with the gaseous halo. Moving beyond spherically symmetric radial profiles, we study the angular dependence of CGM properties around z = 0 massive galaxies in the IllustrisTNG simulations. We characterize the angular signal of density, temperature, and metallicity of the CGM as a function of galaxy stellar mass, halo mass, distance, and SMBH mass, via stacking. TNG predicts that the CGM is anisotropic in its thermodynamical properties and chemical content over a large mass range, $M_*\sim 10^{10-11.5}\, \mathrm{M}_\odot$. Along the minor axis directions, gas density is diluted, whereas temperature and metallicity are enhanced. These feedback-induced anisotropies in the CGM have a magnitude of 0.1−0.3 dex, extend out to the halo virial radius, and peak at Milky Way-like masses, $M_*\sim 10^{10.8}\, \mathrm{M}_\odot$. In TNG, this mass scale corresponds to the onset of efficient SMBH feedback and the production of strong outflows. By comparing the anisotropic signals predicted by TNG versus other simulations – Illustris and EAGLE – we find that each simulation produces distinct signatures and mass dependencies, implying that this phenomenon is sensitive to the underlying physical models. Finally, we explore X-ray emission as an observable of this CGM anisotropy, finding that future X-ray observations, including the eROSITA all-sky survey, will be able to detect and characterize this signal, particularly in terms of an angular modulation of the X-ray hardness.
Anti-Ku antibody-positive myositis presenting as a wide range of axial myopathies and myocarditis: A case report and review of the literature
Authors: Kono Masanori , Komai Toshihiko , Yuki Hayato , Hanata Norio , Kakumoto Toshiyuki , Kubota Akatsuki , Hashimoto Maeda Meiko , Toda Tatsushi , Shoda Hirofumi , Fujio Keishi 
Published: September 2021

ABSTRACT
Idiopathic inflammatory myopathies (IIMs) are autoimmune diseases predominantly affecting proximal muscles; paraspinal muscle involvement is relatively rare. Because paraspinal myopathies do not always cause clinically evident symptoms, the diagnosis of IIMs with axial myopathies can be challenging. Anti-Ku autoantibodies, initially reported in polymyositis/systemic sclerosis overlap syndrome, are myositis-associated antibodies observed in patients with a wide variety of connective tissue diseases. Few reports have been published demonstrating predominant axial myopathy in IIM patients with anti-Ku antibodies. Herein, we investigated a previously healthy Japanese woman in her early 70s who presented with Raynaud’s phenomenon, back pain, and exertional dyspnoea. The creatine kinase was elevated and antinuclear antibody staining was positive, but myositis-specific antibodies were negative. Magnetic resonance imaging revealed myocarditis and a wide range of axial muscle inflammation, including bilateral thoracolumbar paraspinal, infraspinatus, and trapezius muscles. The muscle biopsy was consistent with IIM. In addition, anti-Ku antibody was positive. The administration of prednisolone and tacrolimus quickly alleviated the symptoms, and the creatine kinase level returned to normal. The diagnosis of IIM was arduous in this case because she did not present with camptocormia, muscle weakness involving the proximal limbs was not apparent, and myositis-specific antibodies were negative. Whether axial myopathy and myocarditis are more prevalent in IIM patients with than without anti-Ku antibodies is uncertain. Clinicians should suspect axial myopathy and myositis-associated antibodies, such as anti-Ku antibodies, especially in patients in whom muscle weakness of the proximal limbs is not noticeable.
CPLM 4.0: an updated database with rich annotations for protein lysine modifications
Authors: Zhang Weizhi , Tan Xiaodan , Lin Shaofeng , Gou Yujie , Han Cheng , Zhang Chi , Ning Wanshan , Wang Chenwei , Xue Yu 
Published: September 2021

Abstract
Here, we reported the compendium of protein lysine modifications (CPLM 4.0, http://cplm.biocuckoo.cn/), a data resource for various post-translational modifications (PTMs) specifically occurred at the side-chain amino group of lysine residues in proteins. From the literature and public databases, we collected 450 378 protein lysine modification (PLM) events, and combined them with the existing data of our previously developed protein lysine modification database (PLMD 3.0). In total, CPLM 4.0 contained 592 606 experimentally identified modification events on 463 156 unique lysine residues of 105 673 proteins for up to 29 types of PLMs across 219 species. Furthermore, we carefully annotated the data using the knowledge from 102 additional resources that covered 13 aspects, including variation and mutation, disease-associated information, protein-protein interaction, protein functional annotation, DNA & RNA element, protein structure, chemical-target relation, mRNA expression, protein expression/proteomics, subcellular localization, biological pathway annotation, functional domain annotation, and physicochemical property. Compared to PLMD 3.0 and other existing resources, CPLM 4.0 achieved a &gt;2-fold increase in collection of PLM events, with a data volume of ∼45GB. We anticipate that CPLM 4.0 can serve as a more useful database for further study of PLMs.
Hepatoid adenocarcinoma of the stomach with PIK3Ca mutation during pregnancy: A case report with molecular profile
Authors: Ranceva Anastasija , Stulpinas Rokas , Norvilas Rimvydas , Mickys Ugnius 
Published: September 2021

ABSTRACT
Hepatoid adenocarcinoma is an extremely aggressive special subtype of gastric tumors. It can be lethal as no standard treatment options for this type of gastric cancer exist. Here, we describe a very rare case of a young female on her 21st week of pregnancy who was diagnosed with stage IV hepatoid adenocarcinoma of the stomach with elevated α fetoprotein (AFP) level. Gene mutation analysis performed by next-generation sequencing identified somatic mutations in the PIK3CA gene. Despite the treatment, patient died 2 months after the initial disease presentation. To our best knowledge, this case represents the first report of pregnancy-associated hepatoid gastric adenocarcinoma with the PIK3CA gene mutations, which can provide further clues for the understanding of molecular features of this type of tumor that can reflect biological behavior and may lead to further effective treatment options.
‘Unique does not mean impossible: infant presenting with complicated course of ulceroglandular tularemia.’
Authors: Miącz Karolina , Śledź Jakub , Karwacki Marek W 
Published: September 2021

ABSTRACT
Tularemia is a disease caused by Francisella tularensis—gram-negative coccobacillus. The ulceroglandular type characterized by skin ulcers and painful regional adenopathy is recognized as the most common.
A 1-year-old patient was admitted with severe normocytic anemia, high fever and hepatosplenomegaly. A nonspecific lesion in the axillary region with a homogenous nodal reaction was found, combined with a history of a tick-bite in the pectoral muscle. Primary differentiation included leukemia, lymphoma, mononucleosis, borrelial lymphoma and simple abscess. All of the above were excluded. A further search for diagnosis focused on tick-borne diseases: TIBOLa or anaplasmosis. The ulceroglandular tularemia was eventually confirmed serologically.
Besides the fact that tularemia is a rare diagnosis nowadays, it is still necessary to include this disease in the differentiation of a nonresolving tick-bite abscess with lymphadenopathy. Diagnostic vigilance is the key to effective treatment because other obvious symptoms such as severe anemia might delay the diagnosis.
$SU(5)_{L} \times U(1)_{Y}$
electroweak unification
Authors: Palcu Adrian 
Published: September 2021

Abstract
We propose here—for the first time in the literature, to our best knowledge—an electroweak unification based on the $SU(5)_{L}\times U(1)_{Y}$ gauge group. The spontaneous symmetry breaking takes place in the manner $SU(5)_{L}\times U(1)_{Y} \rightarrow U(1)_{em}$, due to a particular Higgs sector consisting of five scalar quintuplets. Each scalar quintuplet acquires its own vacuum expectation value, by means of a proper parametrization which is worked out once an overall vacuum expectation value in the model is established. The decoupling of the low-energy regime (corresponding to the Standard Model) from the high scale (required by our model here) is straightforwardly achieved in order to preserve the consistency with the present experimental data. Finally, a promising phenomenological outcome is derived by simply tuning a single free parameter. Our results also include, besides a viable one-parameter mass spectrum, the prediction of precisely three generations in the fermion sector and the electric charge quantization.
Heterogenous electrophysiological responses of functionally distinct striatal subregions to circadian and sleep-related homeostatic processes
Authors: Fifel Karim , Deboer Tom 
Published: September 2021

AbstractBasal ganglia (BG) are a set of subcortical nuclei that are involved in the control of a wide variety of motor, cognitive, and affective behaviors. Although many behavioral abnormalities associated with BG dysfunction overlap with the clinical picture precipitated by the lack of sleep, the impact of sleep alterations on neuronal activity in BG is unknown. Using wild-type C57BI mice, we investigated the circadian and sleep-related homeostatic modulation of neuronal activity in the three functional subdivisions of the striatum (i.e. sensorimotor, associative, and limbic striatum). We found no circadian modulation of activity in both ventral and dorsomedial striatum while the dorsolateral striatum displayed a significant circadian rhythm with increased firing rates during the subjective dark, active phase. By combining neuronal activity recordings with electroencephalogram (EEG) recordings, we found a strong modulation of neuronal activity by the nature of vigilance states with increased activity during wakefulness and rapid eye movement sleep relative to nonrapid eye movement sleep in all striatal subregions. Depriving animals of sleep for 6 h induced significant, but heterogenous alterations in the neuronal activity across striatal subregions. Notably, these alterations lasted for up to 48 h in the sensorimotor striatum and persisted even after the normalization of cortical EEG power densities. Our results show that vigilance and sleep states as well as their disturbances significantly affect neuronal activity within the striatum. We propose that these changes in neuronal activity underlie both the well-established links between sleep alterations and several disorders involving BG dysfunction as well as the maladaptive changes in behavior induced in healthy participants following sleep loss.
Physiological responses of Douglas-fir to climate and forest disturbances as detected by cellulosic carbon and oxygen isotope ratios
Authors: Lee Edward Henry , Beedlow Peter A , Brooks J Renée , Tingey David T , Wickham Charlotte , Rugh William 
Published: September 2021

AbstractSwiss needle cast (SNC), caused by a fungal pathogen, Nothophaeocryptopus gaeumannii, is a major forest disease of Douglas-fir (Pseudotsuga menziesii) stands of the Pacific Northwest (PNW). There is mounting concern that the current SNC epidemic occurring in Oregon and Washington will continue to increase in severity, frequency and spatial extent with future warming. Nothophaeocryptopus gaeumannii occurs wherever its host is found, but very little is known about the history and spatial distribution of SNC and its effects on growth and physiological processes of mature and old-growth forests within the Douglas-fir region of the PNW. Our findings show that stem growth and physiological responses of infected Douglas-fir to climate and SNC were different between sites, growth periods and disease severity based on cellulosic stable carbon and oxygen isotope ratios and ring width data in tree rings. At a coastal Oregon site within the SNC impact zone, variations in stem growth and Δ13C were primarily influenced by disproportional reductions in stomatal conductance (gs) and assimilation (A) caused by a loss of functioning stomates through early needle abscission and stomatal occlusion by pseudothecia of N. gaeumannii. At the less severely infected inland sites on the west slopes of Oregon’s Cascade Range, stem growth correlated negatively with δ18O and positively with Δ13C, indicating that gs decreased in response to high evaporative demand with a concomitant reduction in A. Current- and previous-years summer vapor pressure deficit was the principal seasonal climatic variable affecting radial stem growth and the dual stable isotope ratios at all sites. Our results indicate that rising temperatures since the mid-1970s has strongly affected Douglas-fir growth in the PNW directly by a physiological response to higher evaporative demand during the annual summer drought and indirectly by a major SNC epidemic that is expanding regionally to higher latitudes and higher elevations.
Genomic analysis of human noroviruses using combined Illumina–Nanopore data
Authors: Flint Annika , Reaume Spencer , Harlow Jennifer , Hoover Emily , Weedmark Kelly , Nasheri Neda 
Published: September 2021

Abstract
Whole-genome sequence analysis of noroviruses is routinely performed by employing a metagenomic approach. While this methodology has several advantages, such as allowing for the examination of co-infection, it has some limitations, such as the requirement of high viral load to achieve full-length or near full-length genomic sequences. In this study, we used a pre-amplification step to obtain full-length genomic amplicons from 39 Canadian GII isolates, followed by deep sequencing on Illumina and Oxford Nanopore platforms. This approach significantly reduced the required viral titre to obtain full-genome coverage. Herein, we compared the coverage and sequences obtained by both platforms and provided an in-depth genomic analysis of the obtained sequences, including the presence of single-nucleotide variants and recombination events.
Leveraging Telemedicine for Quality Improvement Video Review of Critical ICU Events: A Novel Multidisciplinary Form of Education
Authors: Gold Andrew K. , Huffenberger Ann , Lane-Fall Meghan , Pascual Lopez Jose L. , Rock Kristen C. 
Published: September 2021

Objectives:
The objectives of this study were to codify the events triggering bedside recording and to report the types of performance issues identified that were then used to inform dedicated ICU quality improvement efforts.

Design:
This is a retrospective descriptive analysis of a video review program conducted at a single institution from July 2016 to November 2019.

Setting:
Surgical and Trauma ICU at a single urban academic quaternary care center.

Patients:
All patients admitted to the surgical and trauma ICU between July 2016 and November 2019 were eligible for the study as all ICU beds in our health system institutions are equipped with closed circuit video/audio monitoring. Through an institutional review board approved program, any event triggering the immediate bedside presence of a provider in the ICU is routinely recorded at the discretion of the care team or tele-intensivist.

Interventions:
A database of these events was created over a 3-year period, and cases were reviewed for content, quality improvement, and educational opportunities. Select recordings were analyzed and shared at multidisciplinary/multiprofessional video review sessions.

Measurements and Main Results:
There were 286 critical events video recorded and reviewed in the ICUs between July 2016 and November 2019. The most commonly recorded events included: cardiopulmonary arrests (n = 75), intubations (n = 71), and acute clinical decompensation triggered by nonreassuring vital signs (n = 57) or arrhythmias (n = 13). Of these recordings, 59 were shared at video review conferences, where quality of care was assessed and thematic opportunities for improvement were characterized. Recurrent quality improvement themes that were identified included adherence to protocols, the importance of teamwork and closed-loop communication, clearly designated team leadership, and the use of universal precautions.

Conclusions:
Video review in the ICU is feasible and presents valuable opportunities for quality improvement and educational discussions.