bluuebunny/crossref_metadata_2025_split huggingface dataset:
Perceived Risk and Severity of the Uptake of Safe Male Circumcision Services among Young Men Aged 15-24 Years in Rhino Camp Refugees Settlement, Arua District- Uganda
Authors: David Blate M. , Bandaru Juliet M. , Namajja Khawa E. , Kajoba David , Atuhaire Shallon 
Published: May 2021

Abstract
Background
Safe male circumcision is a proven cost-effective intervention in reducing the risk of sexual transmission of Human Immunodeficiency Virus /Acquired Immune Deficiency Syndrome among heterosexual individuals by 60%. This study aimed at exploring the perceived risk and severity to the uptake of safe male circumcision among young men aged 15–24 years in Rhino Camp Refugees Settlement, Arua District, Uganda.
Methods
This was a cross-sectional study among 378 respondents, which utilized both quantitative and qualitative approaches with a semi-structured and three focus group discussions conducted among young men aged 15–24 years. Univariate analysis was used to determine the uptake of safe male circumcision. Chi-square test and binary regression model were used to determine the odds ratio at 95% confidence levels of safe male circumcision uptake and other exposure variables. Results from the focus group discussions were analysed thematically.
Results
The prevalence of safe male circumcision uptake was 42.1%. The major factor associated with increased likelihood of safe male circumcision uptake was the perceived benefit of cervical cancer prevention in my partner (AOR = 2.455, 95% CI = 1.278–3.627). Factors associated with reduced likelihood safe male circumcision uptake included: fear of perceived increased risk of meatitis (AOR = 0.726, 95% CI = 0.338- .959), fear that undergoing safe male circumcision is very painful and uncomfortable (AOR = 0.742, 95%, CI = 0.279–1.973). Also, the perception that safe make circumcision is not affordable at most private health facilities (AOR = 0.167, 95%, CI = 0.058–0.478).
Conclusions
Safe make circumcision uptake in Rhino Refugees Settlement is lower than Uganda’s national target of 80%. This is attributed to the perceived risks such as perceived risk of infection, pain, irritability, and costs involved. Awareness creation on myths and misconceptions is key to increasing up take of safe make circumcision.
Effects of Early Respiratory Physiotherapy on Spontaneous Respiratory Activity of Preterm Infants: Study Protocol for a Randomized Controlled Trial
Authors: Polito Alessia Di , Vecchio Arianna Del , Tana Milena , Papacci Patrizia , Vento Anna Laura , Campagnola Benedetta , Celona Sefora , Cricenti Laura , Bastoni Ilaria , Tirone Chiara , Lio Alessandra , Aurilia Claudia , Bottoni Anthea , Paladini Angela , Cota Francesco , Ferrara Paola Emilia , Ronconi Gianpaolo , Vento Giovanni 
Published: May 2021

Abstract
Background: Tactile maneuvers stimulating spontaneous respiratory activity in preterm infants are recommended since birth, but data on how and how often these maneuvers are applied in clinical practice are unknown. In the last years, most preterm newborns with respiratory failure are preferentially managed with non-invasive respiratory support and by stimulating spontaneous respiratory activity from the delivery room and in Neonatal Intensive Care Unit (NICU), in order to avoid the risks of intubation and prolonged mechanical ventilation.Methods: Preterm infants with gestational age ≤ 30 weeks not intubated in the delivery room and requiring non-invasive respiratory support at birth will be eligible for the study. They will be randomized and allocated to one of two treatment groups: 1) the Study Group infants will be subject to the technique of respiratory facilitation within the first 24 h of life, according to the reflex stimulations, by the physiotherapist. The newborn is placed in supine decubitus and a slight digital pressure is exerted on a hemithorax. The respiratory facilitation technique will be performed for about three minutes and repeated for a total of 4/6 times in sequence, three times a day until spontaneous respiratory activity is achieved, thus no respiratory support is required; 2) the Control Group Infants will take part exclusively in the Individualized Postural Care program. They will perform the technique of respiratory facilitation and autogenous drainage. The primary outcome of the study will be the incidence of intubation and mechanical ventilation in the first week of life. Discussion: The technique of respiratory facilitation is based on reflex stimulations, applied early to preterm infant. Slight digital pressure is exerted on a "trigger point” of each hemithorax, to stimulate the respiratory activity with subsequent increase of the ipsilateral pulmonary minute ventilation and to facilitate the contralateral pulmonary expansion. This mechanism will determine the concatenation of input to all anatomical structures in relation to the area being treated, to promote spontaneous respiratory activity and reducing work of breathing, avoiding or minimizing the use of invasive respiratory support.Trial registration: UMIN-CTR Clinical Trial, Identifier: UMIN000036066, Registered March 1, 2019. Protocol 1. https://www.umin.ac.jp/ctr
Multiple clonal types of nonvaccine serotypes constitute β-lactams nonsusceptible pneumococcus isolates from adult pneumonia patients in Japan
Authors: Kakiuchi Satoshi , Dhoubhadel Bhim Gopal , Suzuki Motoi , Ishifuji Tomoko , Watanabe Kiwao , Parry Christopher M , Morimoto Konosuke , Ariyoshi Koya 
Published: May 2021

Abstract
Background and objective The global spread of antimicrobial-nonsusceptible Streptococcus pneumoniae is a major concern. Molecular epidemiology of those strains in relation to vaccine serotype remains to be explored in Japan. This study aimed to elucidate the distribution of molecular types with the serotypes and antimicrobial susceptibility of pneumococcus strains isolated from adult pneumonia patients. Methods We enrolled adult pneumonia patients from four sites in Japan between September 2011 and August 2014. S. pneumoniae isolates from sputum and blood were analyzed for serotyping by the Quellung reaction and for antimicrobial susceptibility by the agar dilution method and e-test and for multilocus sequence typing. Results In total, 204 isolates were analyzed from 200 patients with a median age of 72.5 years, of whom 55 (27.5%) patients had received a 23-valent pneumococcal polysaccharide vaccine (PPSV23). We detected 41 clonal complexes (CCs) and 62 sequence types (STs), including 10 new STs: CC/ST 180 of serotype 3 was the most common followed by CC/ST 236 of serotype 19F and CC/ST 99 of serotype 11A; 144 (70.6%) isolates were PPSV23 serotypes; 40 (19.6%) and 121 (59.3%) isolates were b-lactam nonsusceptible (bNS) and multidrug-nonsusceptible strains, respectively. Among the bNS strains, 18 (45%) were nonvaccine serotypes, and 4 CCs (CC236, CC63, CC242, and CC558) comprised of 62.5% of them.Conclusion Multiple CCs of bNS strains, including nonvaccine serotype are spreading. It is crucial to monitor the antimicrobial susceptibility, serotypes, and molecular types of pneumococci to predict the effectiveness of vaccines in preventing pneumonia by bNS pneumococci strains.
Effectiveness of Kangaroo Mother Care by Role-Playing Method on Mother’s Resilience and Breastfeeding Self-Efficacy in Neonatal Intensive Care Unit, a Randomized Controlled Trial
Authors: mohammadi fateme , Basiri Behnaz , Barati Majid , Khazaei Salman , Masoumi Seyedeh Zahra , Sadeghian Efat , Gillespie Mark , Oshvandi Khodayar 
Published: May 2021

Abstract
Background

The purpose of the study is to evaluate the effectiveness of kangaroo mother care by role-playing method on mother’s resilience and breastfeeding self-efficacy in neonatal intensive care unit.
Methods

In this one-blind a randomized controlled trial,78 mothers of infants admitted to neonatal intensive care unit were randomly assigned into two groups: role-playing method (n = 39), routine method (n = 39). The mothers of the intervention group were trained individually in two 30-minute sessions. After training, mothers performed kangaroo mother Care for their infants three times a day and each time 30 min for 7 days. Mothers in the control group received routine embrace care training (booklet training). Questionnaires were collected before the intervention, one day after and one week after intervention. Data analyses were conducted using SPSS version 22.
Results

mother’s resilience and breastfeeding self-efficacy in the study groups was significant at the end of the two among groups. Funding demonstrated a statistically significant difference in the score of the resilience (P = < 0.001) and breastfeeding self-efficacy (P = < 0.001) in the each group one day and one week after performing the intervention. In addition, revealed a statistically significant difference between the both groups in of resilience score(P < 0.01) and breastfeeding self-efficacy (P < 0.01) one day and one week after performing the intervention (P < 0.01), So that the role playing method was more effective than the routine method (booklet training).
Conclusion

Training kangaroo mother care by role-playing and routine methods were both effective in mother’s resilience and breastfeeding self-efficacy, but role-playing method was More effective and can be recommended role playing and routine methods as therapeutic care methods in clinical settings at the beginning of the admission of infants in the neonatal intensive care unit to improve mother’s resilience and breastfeeding self-efficacy.
Can Fetal Weight Discordancy Measured by Ultrasound During Pregnancy Predict Maternal Complications and Neonatal Outcomes in Dichorionic Diamniotic Twins?
Authors: Kim Hyun Mi , Cha Hyun-Hwa , Seong Won Joon , Kim Mi Ju 
Published: May 2021

Abstract
Purpose: The aim of this study was to determine the relationships between the estimated fetal weight discordancy, which was measured by ultrasound during pregnancy, and maternal pregnancy complications and neonatal outcomes in dichorionic diamniotic twin pregnancies.Methods: We conducted a retrospective review of the medical records of 320 twin pregnancies delivered at Chilgok Kyungpook National University Hospital between January 2011 and February 2020. This study included dichorionic diamniotic twin mothers who delivered between 32+1 and 38+0 weeks of gestation. Mothers who had one fetal demise, a major anomaly, or twin-specific complications were excluded. At 20–24 weeks and 28–32 weeks of gestation, participants were divided into 2 groups: discordant twins with an estimated fetal weight difference of more than 20% and concordant twins with a weight difference of less than 20%. The maternal complications and neonatal outcomes were compared between the two groups. Results: The incidences of preeclampsia and placenta previa were significantly higher in discordant twins measured between 20 and 24 weeks compared with concordant twins, but no statistical significance was found in the neonatal outcomes between the two groups. Delivery times were earlier and neonatal weights were lower in discordant twins measured between 28 and 32 weeks. Neonatal outcomes such as ventilator use and neurodevelopment were also significantly different. Conclusion: Discordance in estimated fetal weight measured by ultrasound between 20 and 24 weeks is a risk factor for maternal preeclampsia and placenta previa, whereas discordancy at 28–32 weeks can predict poor neonatal outcomes.
Comprehensive Analysis of The Significance of Hypoxia-Related Genes in Ovarian Cancer
Authors: Zhao Wancheng , Yin Lili 
Published: May 2021

Abstract
Background: Hypoxia-related genes have been reported to play important roles in a variety of cancers. However, their roles in ovarian cancer (OC) have remained unknown. The aim of our research was to explore the significance of hypoxia-related genes in OC patients.Methods: In this study, 15 hypoxia-related genes were screened from The Cancer Genome Atlas (TCGA) database to group the ovarian cancer patients using the consensus clustering method. Principal component analysis (PCA) was performed to calculate the hypoxia score for each patient to quantify the hypoxic status. Results: The OC patients from TCGA-OV dataset were divided into two distinct hypoxia statuses (cluster.A and cluster.B) based on the expression level of the 15 hypoxia-related genes. Most hypoxia-related genes were expressed more highly in the cluster.A group than in the cluster.B group. We also found that patients in the cluster.A group exhibited higher expression of immune checkpoint-related genes, epithelial-mesenchymal transition-related genes, and immune activation-related genes, as well as elevated immune infiltrates. PCA algorithm indicated that patients in the cluster.A group had higher hypoxia scores than that in in the cluster.B group.Conclusions: In summary, our research elucidated the vital role of hypoxia-related genes in immune infiltrates of OC. Our investigation of hypoxic status may be able to improve the efficacy of immunotherapy for OC.
Mental Healthcare-Seeking Behavior during The Perinatal Period among Rural Women in Bangladesh
Authors: Dutta Goutam Kumar , Sarker Bidhan Krishna , Ahamed Helal Uddin , Bhattacharyya Dipika Shankar , Rahman Musfikur , Biswas Taposh Kumar 
Published: May 2021

Abstract
Introduction: Mental health conditions are of rising concern due to their increased contribution to the global burden of disease. Mental health issues are inextricably linked with other sociocultural and health dimensions, especially in the rural areas in developing countries. The complex relationship between mental health issues and sociocultural settings may largely toll upon the healthcare-seeking behavior. Evidence suggests that mental illness affects more than 10% of women and one year after childbirth. So, it urges to document the current status of mental healthcare-seeking behavior during the perinatal period among rural women in Bangladesh to develop a context-specific intervention in the future.Methods: This study was carried out in one sub-district in Bangladesh from April 2017 to June 2018. We conducted 21 In-depth Interviews and seven Focus Group Discussions with different groups of purposively selected participants such as perinatal women, head of the family, community stakeholders, and community level healthcare providers. After collecting the recorded interview and making the verbatim transcription, the data were coded through Atlasti 5.7.a. Data were analyzed thematically to explain the findings.Results: Most of the women with mental disorders at the community level did not seek healthcare during the perinatal period. Women with mental illness also did not know who and where the mental health services are provided. The study found that only one respondent out of twenty-one sought maternal mental healthcare from a gynecologist from a private hospital. In this regard, socio-cultural factors such as social stigma traditional beliefs and practices, social and religious taboos, and social capital also negatively influence healthcare-seeking behaviors. Besides, the community-level service providers were not trained and did not have any guidelines regarding its proper management.Conclusion: The study findings provide us evidence that there is an urgent need to increase the awareness for service users and formulate a guideline for the community-level service provider to manage maternal mental problems during the perinatal period of women in rural Bangladesh.
Molecular Characterization of Isolated Chicken Infectious Anemia Viruses in Central China
Authors: Wang Xinwei , Feng Jie , Liu Ying , Sun Aijun , Liu Huayuan , Wang Xiangru , Wang Jingjing , Wang Rui , Zhu Xiaojing , Xu Yijie , Qin Jinglin , Yang Xia , Chen Lu , Zhuang Guoqing 
Published: May 2021

Abstract
Background: Chicken infectious anemia (CIA) is an immunosuppressive disease induced by the chicken infectious anemia virus (CIAV) causing heavy economic losses once outbreak. This study conducted a systematic analysis of the epidemiology and pathology of CIA in Henan province, central China.Methods: A total of 437 clinical tissue samples and 120 poultry disease-related live attenuated vaccines were collected during 2017-2019; of which 45 were positive for CIAV nucleic acid, with a positive rate of 8.08%. Results: Our results showed that genome sequences similarity among a total of 12 CIAV isolates was high, and ranged from 97.1% to 99.3%, and their similarity to the vaccine strains Cux-1 and Del-Ros ranged from 97.8% to 98.6%. However, There were non-synonymous amino acid mutations in the locus of the major capsid proteins VP1, VP2 and VP3 among all isolates. The subsequent sequence analysis indicated that the isolates of HN-4 and HN-8 showed genetic recombination and follow up animal experiments revealed that HN-4 might be a high pathogenic strain. Conclusions: Our results reveal that both field infection and vaccine contamination promote epidemiology of CIAV in China. Some dominant epidemic viruses have undergone recombination and evolution. This study provides important information for CIAV prevention and control in poultry industry development.
Lonosphere-thermosphere Relation as Seen in Measured and Modeled Electron and Neutral Densities
Authors: Vielberg Kristin , Corbin Armin , Kusche Jürgen , Xiong Chao , Stolle Claudia 
Published: May 2021

Abstract
The availability of in-situ neutral and electron densities along the orbit of the satellite missions GRACE and CHAMP provide a good opportunity to study the ionosphere-thermosphere (IT) system. The aim of this paper is (1) to use these data sets, to study the IT density relation empirically via correlation properties for different conditions depending on solar activity, geomagnetic latitude, and local time and (2) to verify whether these relations are consistent with the output of the TIE-GCM model of the thermosphere and ionosphere.
Our results show that the correlations of electron and neutral densities strongly depend on magnetic local time (MLT) with a minimal correlation between 6-9h MLT, e.g., every 131 days for CHAMP around 400km altitude and every 160 days for GRACE around 500km. During low solar activity, the correlation of modeled and measured densities agrees well for both satellites. On the contrary, we note that the correlations between the modeled values are higher, especially during high solar activity, where the difference between correlations of modeled and measured densities is about 0.2. We suggest that the reason for this misalignment might be related to the poor representation of the equatorial density anomaly in the model especially during high solar activity.
We believe our results will be useful for studies that aim at assimilating electron densities into a physical model to improve the prediction of neutral densities, since the skill of data assimilation depends to a large extent on the representation of the correlation between both densities.
Computational Identification of Guillain-Barre Syndrome Related Genes by an mRNA Gene Expression Profile and a Protein-Protein Interaction Network
Authors: Wang Chunyang , Liao Shiwei , xu jing 
Published: May 2021

Abstract
In this study, we developed a computational method to identify Guillain–Barré syndrome (GBS) related genes based on (i) a gene expression profile, and (ii) the shortest path analysis in a protein-protein interaction (PPI) network. The mRMR (Maximum Relevance Minimum Redundancy) method was employed to select significant genes from an mRNA profile dataset of GBS patients and healthy controls. The protein products of the significant genes were then mapped to a PPI network generated from the STRING database. Shortest paths were computed and all shortest path proteins were picked out and were ranked according to their betweenness. Related genes of the top-most proteins in the ordered list were then retrieved and were regarded as potential GBS related genes in this study. As a result, totally 30 GBS related genes were screened out, in which 20 were retrieved from PPI analysis of up-regulated expressed genes and 23 were from down-regulated expressed genes (13 overlap genes). GO enrichment and KEGG enrichment analysis were performed respectively. Results showed that there were some overlap GO terms and KEGG pathway terms in both up-regulated and down-regulated analysis, which indicated these terms may play critical role during GBS process. These results could shed some light on the understanding of the Genetic and molecular pathogenesis of GBS disease, providing basis for future experimental biology studies and for the development of effective genetic strategies for GBS clinical therapies.
Co-digestion of hybrid Pennisetum and peanut shell after adding TiO2 nanoparticles: Focusing on the synergistic effects on methane production
Authors: Zhao HongMei , Jiang Meng 
Published: May 2021

Abstract
Anaerobic digestion is a widely accepted method to treat wastes such as peanut shell. The energy and nutrients are simultaneously recovered by this method. The objective of this study was to elucidate the effect of TiO 2 nanoparticles in co-digestion of hybrid Pennisetum and peanut shell under mesophilic conditions. The results demonstrated the methane (CH 4 ) production was improved by adding the TiO 2 nanoparticles. The cumulative gas production is best (up to 11,133.3 mL) by adding 0.15% nano-TiO 2 particles. The microbial community analysis showed that Methanobacterium and Methanosarcina were enriched in the presence of TiO 2 nanoparticles indicating that TiO 2 can improve CH 4 production by stimulating the growth of methanogens.
Comprehensive Analysis of Metabolism-Related lncRNA Related To The Progression And Prognosis In Osteosarcoma From TCGA
Authors: Chen Xingyin , Ye Zhengyun , Lou Pan , Liu Wei , Liu Ying 
Published: May 2021

Abstract
Background: Osteosarcoma is one of the most common malignant neoplasm among children and adolescents. Studies have shown that metabolism-related pathways are more important for the development and metastasis of osteosarcoma. Long non-coding RNA (LncRNA) plays a key role in the occurrence and progression of cancer in a variety of ways, Metabolism-related lncRNA-mediated molecular mechanisms in the regulation of osteosarcoma have not been fully elucidated.Methods: In this study, all metabolic-related mRNAs and metabolic-related LncRNA in osteosarcoma were extracted and identified based on transcriptomic data from the TCGA database. The survival analysis, The univariable and multivariable independent prognostic analysis, The results of gene set enrichment analysis (GSEA) and the nomogram were used to construct a prognosis signature with metabolic LncRNA as prognostic factor.Results: 9 prognostic factors including that LncRNA AC009779.2, LncRNA AL591895.1, LncRNA AC026271.3, LncRNA LPP-AS2, LncRNA LINC01857, LncRNA AP005264.1, LncRNA LINC02454, LncRNA AL133338.1 and LncRNA AC135178.5, respectively. The survival analysis showed that the difference in expression of an individual LncRNA was closely related to poor prognosis in osteosarcoma. The univariable and multivariable independent prognostic analysis showed that the signature had good independent predictive ability for patient survival. The results of gene set enrichment analysis (GSEA) suggest that these predictors may be involved in the metabolism of certain substances or energy in cancer. The nomogram is further drawn for clinical guidance and assistance in clinical decision-making.Conclusions: This study identified multiple metabolic-related lncRNA that can be considered as novel therapeutic targets for osteosarcoma and contribute to better exploring the specific regulatory mechanisms of lncRNA in the metabolism of osteosarcoma.