bluuebunny/crossref_metadata_2025_split huggingface dataset:
Dynamics of Microbial Communities on Eggshells and on Nest Materials During Incubation in the Oriental Tit (Parus Minor)
Authors: Song Hokyung , Lee Keesan , Hwang Injae , Yang Eunjeong , Ha Jungmoon , Kim Woojoo , Park Sungjin , Cho Hyunjoon , Choe Jae Chun , Lee Sang-im , Jablonski Piotr G. 
Published: June 2021

Abstract
Eggshell microbial communities may affect hatching success and nestling’s condition. Nest materials are in direct contact with the eggshells, but the relationships with the eggshell microbiome during incubation have not been fully elucidated. Here, we characterize eggshell and nest-material microbial communities and their changes during incubation in the Oriental Tit (Parus minor). Microbial communities on the nest material were relatively stable and remained distinct from the eggshell communities, and had higher diversity and greater phylogenetic clustering compared to the eggshell communities from the same nest, resulting in lower phylogenetic turnover rate of nest material microbiome during incubation than expected by chance. While the species diversity of both communities did not change during incubation, we found significantly greater changes in the structure of microbial communities on the eggshell than on the nest material. However, eggshell microbiome remained distinct from nest material microbiome, suggesting independent dynamics between the two microbiomes during incubation. We detected an increase in the relative abundance of several bacterial taxa on the eggshell that likely come from the bird’s skin, feathers or cloaca/intestine, which suggests some exchange of bacteria between the incubating bird and the eggshell. Furthermore, incubation appeared to promote the abundance of antibiotic producing taxa on the eggshell, which may hypothetically inhibit growth of many bacteria including pathogenic ones. Our results suggest that the future studies should focus on simultaneous monitoring of absolute abundance as well as relative abundance in communities on eggshells, nest materials and the incubating bird’s body.
Calculation of the Optimal Number of Nurses Based on Nursing Intensity by Patient Classification Groups in General Units in South Korea: a Cross-sectional Study
Authors: Ko Yukyung , Park Bohyun 
Published: June 2021

Abstract
Background: Calculating the accurate number of nursing personnel based on a patient classification system that clearly reflects the nursing needs of patients is a problem directly related to the nursing unit’s budget management, productivity, etc. This study aimed to calculate the total daily nursing workload and the optimal number of nurses per general unit based on the nursing intensity and direct nursing time per inpatient through patient classification.Methods: Three units at one general hospital were investigated. To calculate nursing intensity, patient classification according to nursing needs was performed for over 10 days in each unit in September 2018. The direct and non-direct nursing time and nursing intensity scores were analyzed using descriptive statistics (e.g. frequency, percentage, and average) generated using Microsoft Excel.Results: For the internal medicine unit, the average direct nursing time per patient was 1.0, 1.5, 2.2, and 2.9 hours for Groups 1, 2, 3 and 4, respectively. For the surgical unit, the average direct nursing time per patient was 0.9, 1.4, 2.1, and 2.6 hours for Groups 1, 2, 3, and 4, respectively. For the comprehensive nursing care unit, the average direct nursing time per patient was 0.8, 1.2, 1.7, and 2.2 hours for Groups 1, 2, 3, and 4. The optimal number of nurses was 25 in the internal medicine unit, 37 in the surgical unit, and 22 in the comprehensive nursing unit. There was a shortage of five nurses in the internal medicine unit and nine in the surgical unit.Conclusion: Based on the nursing time according to patient classification groups, this study confirmed that the optimal number of nurses cannot be secured and that the nursing intensity is very high. The results of this study suggest that long-term efforts, such as improving the nursing environment, should be made to secure an optimal number of nurses in various hospital nursing units.
Analysis of Risk Factors of Kawasaki Disease With Coronary Artery Lesions
Authors: Hu Jinling , Ren Weidong 
Published: June 2021

Abstract
Objective:To identify factors predictive of coronary artery lesions (CALs) in children with Kawasaki disease (KD).Methods:The clinical data of 420 children with KD who were hospitalized between January 2018 and December 2020 were retrospectively evaluated after assignment to groups by the presence of coronary artery aneurysm (CAA), coronary artery dilation (CAD), or no CALs. The association between coronary artery damage and patient clinical and laboratory values was investigated by pairwise comparison of the three groups. Univariate and multivariate logistic regression identified independent risk factors. The predictive value of patient variables for development of CAA and CAD was estimated by receiver operating characteristic curve analysis.Results:CALs occurred in 17.6% (94/420) of children with KD. Duration of fever, cervical lymphadenopathy, intravenous immune globulin resistance, immunoglobulin (Ig)A, procalcitonin, hemoglobin (Hb), erythrocyte sedimentation rate (ESR), C-reactive protein, and others differed between patients with CALs and without CALs and were significantly associated with the development of CAA and CAD. The largest area under the curve was for combined CAA or CAD indicators, 0.851 for CAA (sensitivity of 68.09% and specificity of 60.62%) and 0.714 for CAD (sensitivity of 65.96% and specificity of 70.77%). Fever of >7 days before treatment was predictive of severe CAA or CAD.Conclusion:Fever duration, ESR, IgA, Hb, and cervical lymphadenopathy were independent risk factors of CAA; CRP, ESR, and cervical lymphadenopathy were independent risk factors of CAD, with combined factors having increased sensitivity and specificity. Early, active treatment is essential to reduce the occurrence of CALs.
Contrasting Ability of Deep and Shallow Rooting Rice Genotypes to Grow Through Plough Pans Containing Biopores and Cracks
Authors: Islam Md. Dhin , Price Adam H. , Hallett Paul D. 
Published: June 2021

Abstract
Aims Cracks and biopores in compacted soil such as plough pans could aid deep rooting, mitigating constraints to seasonal upland use of paddy fields for rice production. This research investigated how soil macropores through a simulated plough pan affects root growth of contrasting deep and shallow rooting rice genotypes.Methods Deep rooting Black Gora and shallow rooting IR64 rice varieties were grown in packed cores of unsaturated soil in a controlled greenhouse. Artificial biopores and cracks (macropores) were inserted through the plough pan to form treatments with no macropores, biopores, cracks, and combined cracks and biopores. Different root parameters such as root length density (RLD), root volume, root diameter, number of root tips and branches were measured. The number of roots was calculated manually, including the number of roots growing through macropores in the plough pan layer.Results Plough pans with macropores had 25-32 % more roots than with no macropores. RLD was 55 % greater in the plough pan layer if cracks were present compared to biopores. Conversely, RLD was 26 % less in subsoil if the plough pan had cracks compared to biopores. Different root parameters were greatly influenced by the presence of macropores in the plough pan, and deep-rooted Black Gora produced 81% greater RLD, 30 % more root numbers and 103 % more branching than the shallow rooted rice genotype IR64 within the plough pan layer.Conclusions Macropores greatly improve rice root growth through plough pans for a deep rooting but not shallow rooting rice variety. Whereas cracks produce a greater number of roots in the plough pan, biopores result in greater root branching and root numbers deeper in subsoil.
Genetic Variations of Plasmodium Falciparum Histidine-rich Protein 2 and 3 in Assosa Zone, Ethiopia: Its Impact on the Performance of Malaria Rapid Diagnostic Tests
Authors: Alemayehu Gezahegn Solomon , Messele Alebachew , Lopez Karen , Lo Eugenia , Janies Daniel , Golassa Lemu 
Published: June 2021

Abstract
Background: Rapid diagnostic tests (RDT) are commonly used for the diagnosis of Plasmodium falciparum malaria. However, false negative results of RDT caused by genetic variations of P. falciparum histidine-rich protein 2 and 3 genes (pfhrp2/3) threaten existing malaria case management and control efforts. The main objective of this study was to investigate the genetic variations of the pfhrp2/3 genes. Methods: A cross-sectional study was conducted from malaria symptomatic individuals in 2018 in Assosa zone, Ethiopia. Finger prick samples were collected for RDT and microscopic examination of thick and thin blood films. Dried blood spots (DBS) were used for genomic parasite DNA extraction and molecular detection. Amplification of parasite DNA was made by quantitative PCR. DNA amplicons of pfhrp2/3 were purified and sequenced. Results: The PfHRP2 repeat type isolates were less conserved compared to the PfHRP3 repeat type. A total of eleven and eight different PfHRP2 and PfHRP3 amino acid repeat types were identified, respectively. Type 1, 4 and 7 repeats were shared by PfHRP2 and PfHRP3 isolates. Type 2 repeats were found only in PfHRP2, while types 16 and 17 were found only in PfHRP3 with a high frequency in all isolates. 18 novel repeat types were found in PfHRP2 and 13 novel repeat types were found in PfHRP3 in single or multiple copies per isolate. The positivity rate for PfHRP2 RDT was high, 82.9% in PfHRP2 and 84.3 % in PfHRP3 sequence isolate at parasitemia levels >250 parasites/µl. Using the Baker model, 100 % of the isolates in group A and 73.7% of the isolates in group B were predicted to be detected by PfHRP2 RDT at parasitemia level> 250 parasite/μl. Conclusion: The findings of this study indicate the presence of different PfHRP2 and PfHRP3 amino acid repeat including novel repeats in P. falciparum from Ethiopia. These results indicate that there is a need to closely monitor the performance of PfHRP2 RDT associated with the genetic variation of the pfhrp2 and pfhrp3 gene in P. falciparum isolates at the country-wide level.
"More Air – Better Performance – Faster Recovery": Study Protocol for Randomised Controlled Trial of the Effect of Post-stroke Inspiratory Muscle Training for Adults
Authors: Sørensen Susanne Lillelund , Kjeldsen Simon Svanborg , Mortensen Sine Secher , Hansen Ulla Torp , Hansen Dorthe , Pedersen Asger Roger , Pallesen Hanne 
Published: June 2021

Abstract
Background: Stroke results in varying physical, cognitive, emotional and/or social disabilities in the short and long term alike. Motor impairments are important, persistent consequences of stroke and include, among others, decreased respiratory muscle function, decreased ability to expand the thorax and postural dysfunction. These deficits affect the patient's ability to perform daily activities, produce fatigue and reduce endurance and quality of life. Inspiratory muscle training (IMT) aims to improve the strength and endurance of the diaphragm and the external intercostal muscles. The objective of this study was to investigate i) the effect of three weeks of IMT on maximal inspiratory pressure (MIP) in adults having suffered a stroke, and ii) the effects of three weeks of IMT on the degree of dependency in activities of daily living, endurance in gait, fatigue, voice volume, phonation endurance and expiratory function.Methods/Design: This is a randomised controlled trial (RCT) comparing IMT with conventional neurorehabilitation (usual practise). The trial will include 80 patients with reduced MIP hospitalised at a specialised neurorehabilitation hospital in Denmark. The intervention group will receive IMT sessions, exercising at 30% of MIP. Patients in the intervention group will perform two daily sessions (one session of IMT with Threshold IMT consisting of two times 15 inspirations at normal breathing rhythm (5-10 min)), seven days a week for three weeks. Training can be with or without physiotherapist supervision. Study outcomes: MIP assessed by the Power Breath, Functional Independence Measurement, The 6-minute Walk Test, the Fatigue Severity Scale and average voice volume. Expiratory function will be assessed using spirometry. All assessments will be conducted at baseline and three weeks (at termination of the intervention) and three months after the intervention has concluded.Discussion: The primary purpose of this study is to assess the effect of IMT interventions in patients with reduced MIP following stroke; secondarily, to assess the effect of IMT on the degree of dependency in activities of daily living, endurance in gait, fatigue, voice volume, phonation endurance and expiratory capacity. Hereby, this study accepts the call for further research.Trial registration: ClinicalTrials.gov NCT04686019. Registered 28.12.2020, https://www.clinicaltrials.gov/ct2/show/NCT04686019
Higher Long-term Visit-to-Visit Glycemic Variability Predicts New-Onset Atrial Fibrillation in Patients with Diabetes Mellitus
Authors: Hsu Jung-Chi , Yang Yen-Yun , Chuang Shu-Lin , Yu Chih-Chieh , Lin Lian-Yu 
Published: June 2021

Abstract
BackgroundAtrial fibrillation (AF) is prevalent in patients with type 2 diabetes mellitus (T2DM). Glycemic variability (GV) is associated with risk of micro- and macrovascular diseases. However, whether the GV can increase the risk of AF remains unknown.MethodsThe cohort study used a database from National Taiwan University Hospital, a tertiary medical center in Taiwan. Between 2014 and 2019, a total of 27246 adult patients with T2DM were enrolled for analysis. Each individual was assessed to determine the coefficients of variability of fasting glucose (FGCV) and HbA1c variability score (HVS). The GV parameters were categorized into quartiles. Multivariate Cox regression models were employed to estimate the relationship between the GV parameters and the risk of AF, transient ischemic accident (TIA)/ischemic stroke and mortality in patients with T2DM.ResultsThe incidence rates of AF and TIA/ischemic stroke were 21.31 and 13.71 per 1000 person-year respectively. The medium follow-up period was 70.7 months. In Cox regression model with full adjustment, the highest quartiles of FGCV was not associated with increased risk of AF (Hazard ratio (HR): 1.11, 95% confidence interval (CI): 0.96-1.29, p=0.165) or TIA/ischemic stroke (HR: 1.03, 95% CI: 0.82-1.29, p=0.821), but was associated with increased risk of total mortality (HR: 1.34, 95% CI: 1.13-1.60, p<0.001) and non-cardiac mortality (HR: 1.42, 95% CI: 1.17-1.72, p<0.001). The highest HVS was significantly associated with increased risk of AF (HR: 1.29, 95% CI: 1.12-1.48, p<0.001), total mortality (HR: 2.44, 95% CI: 2.04-2.91, p<0.001), cardiac mortality (HR: 1.48, 95% CI: 1.04-2.10, p=0.028) and non-cardiac mortality (HR: 2.83, 95% CI: 2.31-3.14, p<0.001) but was not associated with TIA/ischemic stroke (HR: 1.00, 95% CI: 0.79-1.26, p=0.989). The Kaplan-Meier analysis showed significantly higher risk of AF, cardiac and non-cardiac mortality according to the magnitude of GV(log-rank<0.001). ConclusionsHigher GV is independently associated with the development of new-onset AF in patients with T2DM. Reducing GV may be a potential new therapeutic target to prevent AF.
Bowel Endometriosis Presenting With Massive Ascites Relapses in Ovaries: a Case Report and Literature Review
Authors: Bao Yiting , Huang Wu , Yao Liangqing , Yuan Lei 
Published: June 2021

Abstract
Background: About 10% reproductive aged women are affected by endometriosis. Deep infiltrative endometriosis (DIE) is the most severe form of endometriosis which has a high risk of recurrence. Bowel is the most common extragenital involved organ. The clinical features of bowel endometriosis vary by location, size, and infiltrative depth of lesions, which makes diagnosis and treatment difficult. Endometriosis presenting with massive ascites is rare and hard to diagnose before histopathology. There are no authoritative guidelines on the management of DIE at present. Case presentation: A 37-year-old woman presenting with massive ascites and pelvic mass was diagnosed with bowel endometriosis after laparoscopy. Bowel resection and anastomosis followed by gonadotropin-releasing hormone agonists (GnRH-a) therapy was performed. Two levonorgestrel-releasing intrauterine devices and two levonorgestrel-releasing subcutaneous silastic implants were inserted subsequently. Recurrence was found in bilateral ovaries 25 months after surgery. Cystectomy of bilateral ovarian cysts, bilateral salpingectomy and postoperative GnRH-a therapy was performed. The patient showed no recurrence at follow-up by May 2021 (22 months).Conclusions: In the presence of massive ascites and pelvic mass, DIE should be considered as a differential diagnosis for ovarian cancer. Endometriosis in different organs may have different pathogenesis, which leads to different treatment focus. On the basis of complete resection and postoperative GnRH-a therapy, our application of high dose levonorgestrel-releasing systems creatively improves the treatment effect of bowel endometriosis. The mechanism of recurrent endometriosis that occurs in different organs may relate to lymph node involvement and individual immune state.
The Platelet-activating Factor Receptor’s Association with the Outcome of Ovarian Cancer Patients and its Experimental Inhibition by Rupatadine
Authors: Deuster Eileen , Hysenaj Ivi , Kahaly Maja , Schmoeckel Elisa , Mayr Doris , Beyer Susanne , Kolben Thomas , Hester Anna , Kraus Fabian , Chelariu-Raicu Anca , Burges Alexander , Mahner Sven , Jeschke Udo , Trillsch Fabian , Czogalla Bastian 
Published: June 2021

Abstract
The platelet-activating factor receptor (PAFR) and its ligand (PAF) are important inflammatory mediators that are overexpressed in ovarian cancer. The receptor is an important player in ovarian cancer development. In this study, we aimed to evaluate the prognostic value of PAFR in epithelial ovarian cancer (EOC) and the potential use of its antagonist, Rupatadine as an experimental treatment.
Tissue microarrays of ovarian cancer patients, most markedly those with a non-mucinous subtype, immunohistochemically overexpressed PAFR. Elevated cytoplasmic PAFR expression was found to significantly and independently impair patients’ overall and recurrence-free survival (OS: median 83.48 vs. 155.03 months; p=0.022; RFS: median 164.46 vs. 78.03 months; p=0.015). In vitro, especially the serous ovarian cancer subtypes displayed an elevated PAFR gene and protein expression. siRNA knockdown of PAFR decreased cell proliferation significantly, thus confirming the receptor's protumorigenic effect on ovarian cancer cells. The clinically approved PAFR antagonist Rupatadine effectively inhibited in vitro cell proliferation and migration of ovarian cancer cells. PAFR is a prognostic marker in ovarian cancer patients and its inhibition through Rupatadine may have important therapeutic implications in the therapy of ovarian cancer patients.
A Precise Carbon and Oxygen Abundance Determination in a Hot Jupiter Atmosphere
Authors: Line Michael , Brogi Matteo , Bean Jacob , Gandhi Siddharth , Zalesky Joseph , Parmentier Viven , Smith Peter , Mace Gregory , Mansfield Megan , Kempton Eliza , Fortney Jonathan , Shkolnik Evgenya , Patience Jennifer , Rauscher Emily , Desert Jean-Michel , Wardenier Jooost 
Published: June 2021

Abstract
The origins of gas giant planets orbiting close to their host stars (``hot Jupiters'') remain a mystery despite more than a quarter-century of study (Fortney et al. 2021). The atmospheric compositions of these planets are highly sought after to provide insight to their formation location in protoplanetary disks, how they migrated to be so close to their host stars, and the relative role of solid versus gas accretion during their assembly (Madhusudhan 2019). However, simultaneous, bounded constraints on both carbon and oxygen abundances, which are key for understanding giant planet formation (Oeberg et al. 2011, Mordasini et al. 2016, Madhusudhan et al. 2017,Cridland et al. 2016), have been elusive (Kreidberg et al. 2014,Wakeford et al. 2018,Pelletier et al. 2021). Here, we report precise abundance measurements of both water and carbon monoxide in a hot Jupiter atmosphere via ground-based, high resolution spectroscopy. From these constraints on the primary carbon- and oxygen-bearing molecules, paired with upper limits on other minor volatile elemental carriers, we are able to derive the atmospheric elemental metal enrichment (metallicity) and the carbon-to-oxygen ratio (C/O). The inferred atmospheric metallicity is slightly sub-stellar (-0.48$+0.15/-0.13) and the C/O is consistent with stellar (0.59 ±0.08). The former is suggestive of a metal-depleted atmosphere relative to expectations based on extrapolation from the solar system, indicative of a greater partitioning of metals within the core vs the atmosphere. The C/O constraint rules out gas-dominated accretion followed by disk free migration. Taken together in the context of past inferences, these results point to a diversity of planetary atmospheric compositions in addition to the observed diversity of planetary system architectures.
Serum PTH associated with malnutrition determined by bioelectrical impedance technology in chronic kidney disease patients
Authors: Liu Lilin , Wang Lulu , Wang Xiao , Xiong Mingxia , Cao Hongdi , Jiang Lei , Yang Junwei 
Published: June 2021

Abstract
Background: Chronic malnutrition and cachexia are common in chronic kidney disease (CKD), and importance should be given to these complications because they affect the patient’s quality of life and the prognosis. This sudy analyze the correlation between serum PTH level and nutritional status and body composition of patients with CKD. Methods: CKD patients were enrolled in Center for Kidney Disease, Second Affiliated Hospital of Nanjing Medical University from December 1, 2016 to November 30, 2020. Bioelectrical impedance technology was applied to examine body composition. The characteristics of the body composition were compared among different stage of CKD patients, then the correlation between PTH and the body composition was analyzed. Results: 205 CKD patients were enrolled. Twenty-five patients were in stage 1 or 2 CKD, 78 patients were in stage 3 or 4, 31 patients were in stage 5 without dialysis (referred to as CKD stage 5A), and 71 patients were in stage 5 with dialysis (referred to as CKD stage 5B). Body composition analysis showed that the patients had a phase angle (PA) of 5.02 ± 1.07°, a percentage of body fat (PBF) of 27.74 ± 8.8%, and a skeletal muscle mass index (SMI) of 7.4 ± 1.34 kg/m 2. PBF peaked in the CKD stage 3/4 group and gradually decreased with the progression of CKD. PA and SMI differed significantly between the CKD stage 1/2 and stage 5B groups. The proportion of low SMI did not differ significantly between the CKD stage 1/2 and stage 3/4 groups, but it was obviously higher in the CKD stage 5A and 5B groups. PTH was significantly correlated with BMI, hemoglobin, albumin, total cholesterol, triglycerides, and SMI .Binary logistic regression of low SMI showed that the odds ratio for PTH levels greater than the upper limit of the normal range was 11.769 (p = 0.043, 95% confidence interval: 1.078–128.536), and the model predictive power was 0.986 after correction for age, sex, height, weight, hemoglobin, serum calcium, serum phosphorus, serum total cholesterol, serum triglyceride, and basal metabolic rate. Conclusion: Bioelectrical impedance can effectively assess the nutritional status of CKD patients in terms of fat and muscle parameters. High levels of PTH are an independent risk factor for developing low SMI in CKD patients.