bluuebunny/crossref_metadata_2025_split huggingface dataset:
Auld Lang Syne
Authors: Grant Morag Josephine 
Published: November 2021

In Auld Lang Syne: A Song and its Culture, M. J. Grant explores the history of this iconic song, demonstrating how its association with ideas of fellowship, friendship and sociality has enabled it to become so significant for such a wide range of individuals and communities around the world.
This engaging study traces different stages in the journey of Auld Lang Syne, from the precursors to the song made famous by Robert Burns to the traditions and rituals that emerged around the song in the nineteenth and early twentieth centuries, including its use as a song of parting, and as a song of New Year. Grant’s painstaking study investigates the origins of these varied traditions, and their impact on the transmission of the song right up to the present day.
Grant uses Auld Lang Syne to explore the importance of songs and singing for group identity, arguing that it is the active practice of singing the song in group contexts that has made it so significant for so many. The book offers fascinating insights into the ways that Auld Lang Syne has been received, reused and remixed around the world, concluding with a chapter on more recent versions of the song back in Scotland.
This highly original and accessible work will be of great interest to non-expert readers as well as scholars and students of musicology, cultural and social history, social anthropology and Scottish studies. The book contains a wealth of illustrations and includes links to many more, including manuscript sources. Audio examples are included for many of the musical examples. Grant’s extensive bibliography will moreover ease future referencing of the many sources consulted.
Evaluation of Soil Moisture in CMIP6 Simulations
Authors: Qiao Liang , Zuo Zhiyan , Xiao Dong 
Published: November 2021

Abstract
This study employs multiple reanalysis datasets to evaluate the global shallow and deep soil moisture in Coupled Model Intercomparison Project phase 6 (CMIP6) simulations. The multimodel ensemble mean produces generally reasonable simulations for overall climatology, wet and dry centers, and annual peaks in the melt season at mid- to high latitudes and the rainy season at low latitudes. The simulation capability for shallow soil moisture depends on the relationship between soil moisture and the difference between precipitation and evaporation (P − E). Although most models produce effective simulations in regions where soil moisture is significantly related to the P − E (e.g., Europe, low-latitude Asia, and the Southern Hemisphere), considerable discrepancies between simulated conditions and reanalysis data occur at high elevations and latitudes (e.g., Siberia and the Tibetan Plateau), where cold-season processes play a driving role in soil moisture variability. These discrepancies reflect the lack of information concerning the thaw of snow and frozen ground in the reanalyzed data and the inability of models to simulate these processes. The models also perform poorly in areas of extreme aridity. On a global scale, the majority of models provide consistent and capable simulations owing to the minimal variability in deep soil moisture and limited observational information in reanalysis data. Models with higher spatial resolution do not exhibit closer agreement with the reanalysis data, indicating that spatial resolution is not the first limiting factor for CMIP6 soil moisture simulations.
Climate Modeling in Low Precision: Effects of Both Deterministic and Stochastic Rounding
Authors: Paxton E. Adam , Chantry Matthew , Klöwer Milan , Saffin Leo , Palmer Tim 
Published: November 2021

Abstract
Motivated by recent advances in operational weather forecasting, we study the efficacy of low-precision arithmetic for climate simulations. We develop a framework to measure rounding error in a climate model, which provides a stress test for a low-precision version of the model, and we apply our method to a variety of models including the Lorenz system, a shallow water approximation for flow over a ridge, and a coarse-resolution spectral global atmospheric model with simplified parameterizations (SPEEDY). Although double precision [52 significant bits (sbits)] is standard across operational climate models, in our experiments we find that single precision (23 sbits) is more than enough and that as low as half precision (10 sbits) is often sufficient. For example, SPEEDY can be run with 12 sbits across the code with negligible rounding error, and with 10 sbits if minor errors are accepted, amounting to less than 0.1 mm (6 h)−1 for average gridpoint precipitation, for example. Our test is based on the Wasserstein metric and this provides stringent nonparametric bounds on rounding error accounting for annual means as well as extreme weather events. In addition, by testing models using both round-to-nearest (RN) and stochastic rounding (SR) we find that SR can mitigate rounding error across a range of applications, and thus our results also provide some evidence that SR could be relevant to next-generation climate models. Further research is needed to test if our results can be generalized to higher resolutions and alternative numerical schemes. However, the results open a promising avenue toward the use of low-precision hardware for improved climate modeling.

Significance Statement
Weather and climate models provide vital information for decision-making, and will become ever more important in the future with a changed climate and more extreme weather. A central limitation to improved models are computational resources, which is why some weather forecasters have recently shifted from conventional 64-bit to more efficient 32-bit computations, which can provide equally accurate forecasts. Climate models, however, still compute in 64 bits, and adapting to lower precision requires a detailed analysis of rounding errors. We develop methods to quantify rounding error in a climate model, and find similar precision acceptable across weather and climate models, with even 16 bits often sufficient for an accurate climate. This opens a promising avenue for computational efficiency gains in climate modeling.
Risk Factors for Gastrointestinal Leak after Perforated Peptic Ulcer Disease Operative Repair
Authors: Lund Sarah , Chauhan Kiran Kaur , Zietlow John , Stephens Daniel , Zietlow Scott , Strajina Veljko , Turay David , Zielinski Martin 
Published: November 2021

Background There are limited studies regarding the impact of post-operative leak on perforated peptic ulcer disease (PPUD) and conflicting results regarding routine drain placement in operative repair of PPUD. This study aims to identify risk factors for gastrointestinal leak after operative repair of PPUD to better guide intra-operative decisions about drain placement. Methods We performed a retrospective cohort study at a tertiary care center from 2008 to 2019, identifying 175 patients who underwent operative repair of PPUD. Results Patients who developed a leak (17%) were compared to patients who did not. Both hypoalbuminemia (albumin < 3.5 g/dL) ( P = .03) and duodenal ulcers ( P < .01) were identified as significant risk factors for leak. No significant difference was found between leak and no leak groups for AAST disease severity grade, repair technique, or pre-operative use of tobacco, alcohol, or steroids. Post-operative leaks were associated with prolonged hospital stay (29 days compared to 10, P < .01), increased complication rates (77% compared to 48%, P < .01), and increased re-operation rates (73% compared to 26%, <0.01). No difference was identified in patient characteristics or operative leak rates between patients who had drains placed at the index operation and those that did not. Discussion Leak after operative PPUD repair is associated with significant post-operative morbidity. Hypoalbuminemia and duodenal perforations are significant risk factors for post-operative leaks.
Haemocyte variations in 35 species of grasshoppers and locusts
Authors: Zhang Keshi , Zhang Xiaomin 
Published: November 2021

Introduction: Grasshoppers and locusts are widely distributed worldwide, causing significant losses in agriculture. The origin and functions of their haemocytes are not entirely understood. Objectives: Insect haemocytes arbitrate cellular defence and participate in humoral defences. Due to their importance, the haemocytes of 35 species of grasshoppers and locusts from China were morphologically examined in this study. We aim to highlight a simple method for the morphological examination of insect haemocytes. Methods: The haemocytes were observed, counted and compared under a light microscope after Wright-Giemsa staining. Results: High complexity in form and shape were observed in the haemocytes. These include prohaemocytes, plasmatocytes, granulocytes, vermicytes, podocytes and megakaryocytes. No clear relationship was seen between the haemocyte type and their phylogenetic relationship among the three families examined. The high abundance of plasmatocytes and granulocytes suggests their importance in the immunity of grasshoppers and locusts. The minor haemocyte populations including prohaemocytes, vermicytes and podocytes may not be always present in individuals. Conclusion: All examined species shared similarities in their haemocyte types. Wright-Giemsa staining is a simple and efficient method for evaluating haemocytes.
Sulforaphane suppresses autophagy during the malignant progression of gastric carcinoma via activating miR-4521/PIK3R3 pathway
Authors: Peng Zi-tan , Gu Pei 
Published: November 2021

Objective Sulforaphane, which exerts an effective anti-cancer ability, is a phytochemical converted from cruciferous plants. Here, we aimed to identify whether sulforaphane could suppress autophagy during the malignant progression of gastric carcinoma and to explore the underlying mechanisms. Methods SGC7901 cells were transfected with miR-4521 mimics, inhibitor, and pcDNA3.1- PIK3R3, and treated with sulforaphane or autophagy inhibitor. Cell proliferation, apoptosis, and miR-4521 or PIK3R3 expression were detected. Results MiR-4521 over-expression suppressed LC3-II/I ratio and Beclin-1 expression but induced p62 expression in SGC7901 cells. MiR-4521 also reduced gastric carcinoma cell proliferation and promoted apoptosis in vitro. In the mechanical observation, we identified that miR-4521 directly targeted PIK3R3 to repress its expression, and PIK3R3 up-regulation partly antagonized miR-4521-mediated autophagy, proliferation, and apoptosis in gastric carcinoma cells. In addition, sulforaphane exerted effective anti-cancer functions by repressing autophagy and growth in tumor cells at a concentration-dependent way. MiR-4521 inhibition or PIK3R3 over-expression weakened the anti-cancer functions of sulforaphane in gastric carcinoma cells. Conclusion Consequently, miR-4521 suppressed autophagy during the malignant progression of gastric carcinoma by targeting PIK3R3. Thus, miR-4521 may be applied as a therapeutic target for sulforaphane in gastric carcinoma.
Valproic Acid After Neurosurgery Induces Elevated Risk of Liver Injury: A Prospective Nested Case-Control Study
Authors: Guo Jinlin , Ma Jiuhong , Wang Shan , Li Xingang , Ji Hongming , Li Yuanping , Peng Fangchen , Sun Yiqi 
Published: November 2021

Background Valproic acid (VPA) has been widely used to prevent epileptic seizures after neurosurgery in China. We have found that the incidence of liver injury (LI) in patients using VPA after neurosurgery is higher than that in other patients. Objective The objective of this study was to investigate the risk factors of LI in patients using VPA after neurosurgery. Methods A nested case-control study was conducted in patients using VPA after neurosurgery between September 2019 and March 2021. Cases of LI were matched to controls by age and body mass index (BMI). Conditional logistic regression was used to estimate matched odds ratios representing the odds of LI. A receiver operating characteristic curve was used to analyze the optimal cutoff condition. Results A total of 248 people (62 LI and 186 control) were enrolled. Among patients with vs without LI, the matched odds ratio for trough concentration of VPA was significant (matched odds ratio [OR], 1.09; 95% confidence interval [CI]: 1.01-1.19). The course of treatment (OR: 1.17, 95% CI: 1.02-1.33), Glasgow score (OR: 0.26, 95% CI: 0.10-0.67), gene polymorphisms of CYP2C19 (OR: 2.09, 95% CI: 1.03-146.93), and UGT1A6 (OR: 34.61, 95% CI: 1.19-1003.23) were all related to the outcome. The optimal cutoff of the course of treatment was 10 days, while the trough concentration of VPA was determined to be 66.16 mg/L. Conclusion Length of treatment, VPA trough concentration, and Glasgow score were associated with LI in patients after neurosurgery. A gene test may be necessary for people who are prescribed VPA for a long time.
Tolerability of palbociclib in younger and older patients with advanced breast cancer
Authors: Dennison Taylor , Heiling Hillary , Deal Allison , Brunk Kelly , Kemper Ryan , Crona Daniel J , Faso Aimee 
Published: November 2021

Introduction Palbociclib is a small-molecule cyclin-dependent kinase 4/6 inhibitor used to treat hormone receptor-positive, human epidermal growth factor receptor-2 negative advanced breast cancer. Patient-specific factors impacting dose reductions or discontinuations are unknown. Methods The primary objective was to evaluate the association of age (<60 vs. ≥60 years) with palbociclib dose reductions or discontinuations secondary to neutropenia. This single-center, retrospective chart review included hormone receptor-positive, human epidermal growth factor receptor-2 negative advanced breast cancer patients ≥18 years treated with palbociclib between April 2015 and May 2020. Patients <60 years at the time of palbociclib initiation were in the younger group and patients ≥60 years were in the older group. Results Among the 107 patients included, younger patients were less likely than older patients to have a palbociclib starting dose <125 mg (0% vs. 11.9%, p = 0.02). Differences in palbociclib dose reductions or treatment discontinuations secondary to neutropenia were not detected (35.4% vs. 42.4%, p = 0.55). Neither the total number of palbociclib dose reductions (none: 54.2% vs. 49.1%, one: 33.3% vs. 42.4%, two: 12.5% vs. 8.5%, p = 0.61), nor the final dose of palbociclib (125 mg: 54.2% vs. 40.7%, 100 mg: 29.2% vs. 27.1%, 75 mg: 16.7% vs. 32.2%, p = 0.17) differed between younger and older patients. Conclusions Age (<60 vs. ≥60 years) was not associated with the rate of palbociclib dose reductions or discontinuations secondary to neutropenia. Older (≥60 years) patients were more likely to start palbociclib at lower doses which may impact neutropenia and non-neutropenic intolerance.
Anterior Glenoid Reconstruction With Distal Tibial Allograft: Biomechanical Impact of Fixation and Presence of a Retained Lateral Cortex
Authors: Parada Stephen A. , Shaw K. Aaron , McGee-Lawrence Meghan E. , Kyrkos Judith G. , Paré Daniel W. , Amero Jessica , Going James W. , Morpeth Brice , Shelley Robert , Eichinger Josef K. , Provencher Matthew T. 
Published: November 2021

Background: Glenoid reconstruction with distal tibial allograft (DTA) is a known surgical option for treating recurrent glenohumeral instability with anterior glenoid bone loss; however, biomechanical analysis has yet to determine how graft variability and fixation options alter the torque of screw insertion and load to failure. Hypothesis: It was hypothesized that retention of the lateral cortex of the DTA graft and the presence of a washer with the screw will significantly increase the maximum screw placement torque as well as the load to failure. Study Design: Controlled laboratory study. Methods: Whole, fresh distal tibias were used to harvest 28 DTA grafts, half of which had the lateral cortex removed and half of which had the lateral cortex intact. The grafts were secured to polyurethane solid foam blocks with a 2-mm epoxy laminate to simulate a glenoid with an intact posterior glenoid cortex. Grafts underwent fixation with 4.0-mm cannulated drills, and screws and washers were used for half of each group of grafts while screws alone were used for the other half, creating 4 equal groups of 7 samples each. A digital torque-measuring screwdriver recorded peak torque for screw insertion. Constructs were then tested in compression with a uniaxial materials testing system and loaded in displacement control at 100 mm/min until at least 3 mm of displacement occurred. Ultimate load was defined as the load sustained at clinical failure. Results: The use of a washer significantly improved the ultimate torque that could be applied to the screws (+cortex and +washer = 12.42 N·m [SE, 0.82]; –cortex and +washer = 10.54 N·m [SE, 0.59]) ( P < .0001), whereas the presence of the native bone cortex did not have a significant effect (+cortex and –washer = 7.83 N·m [SE, 0.40]; –cortex and –washer = 8.03 N·m [SE, 0.56]) ( P = .181). Conclusion: In a hybrid construct of fresh cadaveric DTA grafts secured to a foam block glenoid model, the addition of washers was more effective than the retention of the lateral distal tibial cortex for both load to failure and peak torque during screw insertion. Clinical Relevance: This biomechanical study is relevant to the surgeon when choosing a graft and selecting fixation options during glenoid reconstruction with a DTA graft.
How I treat cardiovascular complications in patients with lymphoid malignancies
Authors: Herrmann Joerg , McCullough Kristen B. , Habermann Thomas M. 
Published: November 2021

Abstract
The prognosis of several lymphoid malignancies has improved through development of novel therapies, combination with traditional chemotherapies, and delineation of appropriate therapeutic sequencing. Toxicities that are arising because of prolonged or multiple sequential therapeutic interventions are becoming increasingly impactful. Among the broad spectrum of complications that patients with lymphoid malignancies may experience, cardiovascular toxicities are significant in terms of morbidity and mortality. The entire cardiovascular system can be affected, but cardiomyopathy, heart failure, and arrhythmias remain of greatest concerns with the use of anthracyclines, hematopoietic stem cell transplantation, and radiation therapy in patients with lymphoid malignancies. These aspects will be covered in this article within the framework of case-based discussions. Key to the management of cardiovascular complications in patients with lymphoid malignancies is awareness and preparedness across the cancer continuum. Baseline risk stratification helps to direct surveillance and early intervention efforts before, during, and after cancer therapy, which are paramount for the best possible outcomes. Along these lines, the overall goal is to enable the best possible therapies for lymphoid malignancies without the complications of clinically significant cardiovascular events.
Microlyse: a thrombolytic agent that targets VWF for clearance of microvascular thrombosis
Authors: de Maat Steven , Clark Chantal C. , Barendrecht Arjan D. , Smits Simone , van Kleef Nadine D. , El Otmani Hinde , Waning Manon , van Moorsel Marc , Szardenings Michael , Delaroque Nicolas , Vercruysse Kristof , Urbanus Rolf T. , Sebastian Silvie , Lenting Peter J. , Hagemeyer Christoph , Renné Thomas , Vanhoorelbeke Karen , Tersteeg Claudia , Maas Coen 
Published: November 2021

Abstract
Thrombotic microangiopathies are hallmarked by attacks of disseminated microvascular thrombosis. In thrombotic thrombocytopenic purpura (TTP), this is caused by a rise in thrombogenic ultra-large von Willebrand factor (VWF) multimers because of ADAMTS13 deficiency. We previously reported that systemic plasminogen activation is therapeutic in a TTP mouse model. In contrast to its natural activators (ie, tissue plasminogen activator and urokinase plasminogen activator [uPA]), plasminogen can directly bind to VWF. For optimal efficacy and safety, we aimed to focus and accelerate plasminogen activation at sites of microvascular occlusion. We here describe the development and characterization of Microlyse, a fusion protein consisting of a high-affinity VHH targeting the CT/CK domain of VWF and the protease domain of uPA, for localized plasminogen activation on microthrombi. Microlyse triggers targeted destruction of platelet-VWF complexes by plasmin on activated endothelial cells and in agglutination studies. At equal molar concentrations, Microlyse degrades microthrombi sevenfold more rapidly than blockade of platelet-VWF interactions with a bivalent humanized VHH (caplacizumab*). Finally, Microlyse attenuates thrombocytopenia and tissue damage (reflected by increased plasma lactate dehydrogenase activity, as well as PAI-1 and fibrinogen levels) more efficiently than caplacizumab* in an ADAMTS13−/− mouse model of TTP, without affecting hemostasis in a tail-clip bleeding model. These findings show that targeted thrombolysis of VWF by Microlyse is an effective strategy for the treatment of TTP and might hold value for other forms of VWF-driven thrombotic disease.
Identification of miRNAs and their target genes in genic male sterility lines in Brassica napus by small RNA sequencing
Authors: Jiang Jianxia , Xu Pengfei , Li Yajie , Li Yanli , Zhou Xirong , Jiang Meiyan , Zhang Junying , Zhu Jifeng , Wang Weirong , Yang Liyong 
Published: November 2021

Abstract
Background
Brassica napus is the third leading source of edible oil in the world. Genic male sterility (GMS) lines provide crucial material for harnessing heterosis for rapeseed. GMS lines have been used successfully for rapeseed hybrid production in China. MicroRNAs (miRNAs) play crucial regulatory roles in various plant growth, development, and stress response processes. However, reports on miRNAs that regulate the pollen development of GMS lines in B. napus are few.

Results
In this study, 12 small RNA and transcriptome libraries were constructed and sequenced for the flower buds from the fertile and sterile lines of two recessive GMS (RGMS) lines, namely, “6251AB” and “6284AB”. At the same time, 12 small RNA and transcriptome libraries were also constructed and sequenced for the flower buds from the fertile and sterile lines of two dominant GMS (DGMS) lines, namely, “4001AB” and “4006AB”. Based on the results, 46 known miRNAs, 27 novel miRNAs on the other arm of known pre-miRNAs, and 44 new conserved miRNAs were identified. Thirty-five pairs of novel miRNA-3p/miRNA-5p were found. Among all the identified miRNAs, fifteen differentially expressed miRNAs with over 1.5-fold change between flower buds of sterile and fertile lines were identified, including six differentially expressed miRNAs between “4001A” and “4001B”, two differentially expressed miRNAs between “4006A” and “4006B”, four differentially expressed miRNAs between “6251A” and “6251B”, and ten differentially expressed miRNAs between “6284A” and “6284B”. The correlation analysis of small RNA and transcriptome sequencing was conducted. And 257 candidate target genes were predicted for the 15 differentially expressed miRNAs. The results of 5′ modified RACE indicated that BnaA09g48720D, BnaA09g11120D, and BnaCnng51960D were cleaved by bna-miR398a-3p, bna-miR158-3p and bna-miR159a, respectively. Among the differentially expressed miRNAs, miR159 was chosen to analyze its function. Overexpression of bna-miR159 in Arabidopsis resulted in decreased seed setting rate, and shortened siliques, illustrating that miR159 may regulate the fertility and silique development in rapeseed.

Conclusions
Our findings provide an overview of miRNAs that are potentially involved in GMS and pollen development. New information on miRNAs and their related target genes are provided to exploit the GMS mechanism and reveal the miRNA networks in B. napus.