bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Study of the Pupal Development of Five Forensically Important Flies (Diptera: Brachycera)
Authors: Barros-Cordeiro K B , Pujol-Luz J R , Báo S N 
Published: April 2021

AbstractHolometabolous insects undergo complete metamorphosis, and hence, they have different phases of development (egg, larva, pupa, and adult), which occupy distinct ecological niches. The pupae of several fly species are surrounded by the puparium, which is a rigid structure, usually formed by the integument of the last larval instar. The puparium presents unique characteristics distinct from those of the larval and adult phases. During intrapuparial development, it is possible to distinguish at least four fundamental and continuous steps, namely: 1) larval–pupal apolysis, 2) cryptocephalic pupa, 3) phanerocephalic pupa, and 4) pharate adult. The objective of this work was to describe the external morphology of the distinct phase of development for five species that were collected, identified, and raised in the laboratory; intrapuparial development was studied by fixing immature specimens at regular intervals; the morphological analyses were performed with the aid of both light and scanning electron microscopy. Under the conditions established (27 ± 1.0 or 23 ± 1.0°C, 60 ± 10% relative humidity, 12 h of photoperiod), the minimum time for intrapuparial development was: 252 h for Megaselia scalaris (Loew 1966) (Phoridae), 192 h for Piophila casei (Linnaeus 1758) (Piophilidae), Fannia pusio (Wiedemann 1830) (Fanniidae), and Musca domestica (Linnaeus 1758) (Muscidae), and 96 h for Chrysomya megacephala (Fabricius 1794) (Calliphoridae). Intrapuparial development has defined steps, and distinct species responded differently to the same environmental conditions. In addition, it is possible to establish a sequential rule without ignoring the specific characteristics of each taxon.
Mosquito Identification From Bulk Samples Using DNA Metabarcoding: a Protocol to Support Mosquito-Borne Disease Surveillance in Canada
Authors: Mechai S , Bilodeau G , Lung O , Roy M , Steeves R , Gagne N , Baird D , Lapen D R , Ludwig A , Ogden N H 
Published: April 2021

Abstract
Approximately 80 species of mosquitoes (Diptera: Culicidae) have been documented in Canada. Exotic species such as Aedes albopictus (Skuse) (Diptera: Culicidae) are becoming established. Recently occurring endemic mosquito-borne diseases (MBD) in Canada including West-Nile virus (WNV) and Eastern Equine Encephalitis (EEE) are having significant public health impacts. Here we explore the use of DNA metabarcoding to identify mosquitoes from CDC light-trap collections from two locations in eastern Canada. Two primer pairs (BF2-BR2 and F230) were used to amplify regions of the cytochrome c oxidase subunit I (CO1) gene. High throughput sequencing was conducted using an Illumina MiSeq platform and GenBank-based species identification was applied using a QIIME 1.9 bioinformatics pipeline. From a site in southeastern Ontario, Canada, 26 CDC light trap collections of 72 to &gt;300 individual mosquitoes were used to explore the capacity of DNA metabarcoding to identify and quantify captured mosquitoes. The DNA metabarcoding method identified 33 species overall while 24 species were identified by key. Using replicates from each trap, the dried biomass needed to identify the majority of species was determined to be 76 mg (equivalent to approximately 72 mosquitoes), and at least two replicates from the dried biomass would be needed to reliably detect the majority of species in collections of 144–215 mosquitoes and three replicates would be advised for collections with &gt;215 mosquitoes. This study supports the use of DNA metabarcoding as a mosquito surveillance tool in Canada which can help identify the emergence of new mosquito-borne disease potential threats.
On the Child’s Right to Bodily Integrity: When Is the Right Infringed?
Authors: Mazor Joseph 
Published: April 2021

Abstract
This article considers two competing types of conceptions of the pre-autonomous child’s right to bodily integrity. The first, which I call encroachment conceptions, holds that any physically serious bodily encroachment infringes on the child’s right to bodily integrity. The second, which I call best-interests conceptions, holds that the child’s right to bodily integrity is infringed just in case the child is subjected to a bodily encroachment that substantially deviates from what is in the child’s best interests. I argue in this article that best-interests conceptions are more plausible than encroachment conceptions. They have more attractive implications regarding the permissibility of interventions in children’s bodies that are beneficial for the child but are not medically necessary. They are better able to explain the moral distinction between cases in which an encroachment on a child’s body is needed to benefit that child and cases in which an encroachment on one child’s body is needed to benefit another. Finally, best-interests conceptions are more consonant than encroachment conceptions with our understanding of adults’ right to bodily integrity.
EGFRAmplification in Metastatic Colorectal Cancer
Authors: Randon Giovanni , Yaeger Rona , Hechtman Jaclyn F , Manca Paolo , Fucà Giovanni , Walch Henry , Lee Jeeyun , Élez Elena , Seligmann Jenny , Mussolin Benedetta , Pagani Filippo , Germani Marco Maria , Ambrosini Margherita , Rossini Daniele , Ratti Margherita , Salvà Francesc , Richman Susan D , Wood Henry , Nanjangud Gouri , Gloghini Annunziata , Milione Massimo , Bardelli Alberto , de Braud Filippo , Morano Federica , Cremolini Chiara , Pietrantonio Filippo 
Published: April 2021

AbstractBackgroundEGFR amplification occurs in about 1% of metastatic colorectal cancers (mCRCs) but is not routinely tested as a prognostic or predictive biomarker for patients treated with anti-EGFR monoclonal antibodies. Herein, we aimed to characterize the clinical and molecular landscape of EGFR-amplified mCRC.MethodsIn this multinational cohort study, we compared clinical data of 62 patients with EGFR-amplified vs 1459 EGFR nonamplified mCRC, as well as comprehensive genomic data of 35 EGFR-amplified vs 439 EGFR nonamplified RAS/BRAF wild-type and microsatellite stable (MSS) tumor samples. All statistical tests were 2-sided.ResultsEGFR amplification was statistically significantly associated with left primary tumor sidedness and RAS/BRAF wild-type status. All EGFR-amplified tumors were MSS and HER2 nonamplified. Overall, EGFR-amplified samples had higher median fraction of genome altered compared with EGFR-nonamplified, RAS/BRAF wild-type MSS cohort. Patients with EGFR-amplified tumors reported longer overall survival (OS) (median OS = 71.3 months, 95% confidence interval [CI] = 50.7 to not available [NA]) vs EGFR-nonamplified ones (24.0 months; 95% CI = 22.8 to 25.6; hazard ratio [HR] = 0.30, 95% CI = 0.20 to 0.44; P &lt; .001; adjusted HR = 0.46, 95% CI = 0.30 to 0.69; P &lt; .001). In the subgroup of patients with RAS/BRAF wild-type mCRC exposed to anti-EGFR-based therapy, EGFR amplification was again associated with better OS (median OS = 54.0 months, 95% CI = 35.2 to NA, vs 29.1 months, 95% CI = 27.0 to 31.9, respectively; HR = 0.46, 95% CI = 0.28 to 0.76; P = .002).ConclusionPatients with EGFR-amplified mCRC represent a biologically defined subgroup and merit dedicated clinical trials with novel and more potent EGFR-targeting strategies beyond single-agent monoclonal antibodies.
Fundamentals of the Development of Connectivity in the Human Fetal Brain in Late Gestation: From 24 Weeks Gestational Age to Term
Authors: Kostović Ivica , Radoš Milan , Kostović-Srzentić Mirna , Krsnik Željka 
Published: April 2021

Abstract
During the second half of gestation, the human cerebrum undergoes pivotal histogenetic events that underlie functional connectivity. These include the growth, guidance, selection of axonal pathways, and their first engagement in neuronal networks. Here, we characterize the spatiotemporal patterns of cerebral connectivity in extremely preterm (EPT), very preterm (VPT), preterm and term babies, focusing on magnetic resonance imaging (MRI) and histological data. In the EPT and VPT babies, thalamocortical axons enter into the cortical plate creating the electrically-active, functional synapses. Additionally, the subplate zone gradually resolves in the preterm and term brain in conjunction with the growth of associative pathways leading to the activation of large-scale neural networks. We demonstrate that specific classes of axonal pathways within cerebral compartments are selectively vulnerable to temporally nested pathogenic factors. In particular, the radial distribution of axonal lesions, that is, radial vulnerability, is a robust predictor of clinical outcome. Furthermore, the subplate tangential nexus that we can visualize using MRI could be an additional marker as pivotal in the development of cortical connectivity. We suggest to direct future research toward the identification of sensitive markers of earlier lesions, the elucidation of genetic mechanisms underlying pathogenesis, and better long-term follow-up using structural and functional MRI.
SMARCB1-deficient sinonasal carcinoma: a case report and literature review
Authors: Yanagawa Naoki , Suzuki Masamichi , Sugimoto Ryo , Osakabe Mitsumasa , Uesugi Noriyuki , Shiga Kiyoto , Sugai Tamotsu 
Published: April 2021

Abstract
SWItch/Sucrose Non-Fermentable (SWI/SNF) -related matrix-associated actin-dependent regulator of chromatin (SMARC) subfamily B member 1 (SMARCB1) deficient sinonasal carcinoma (SdSNC) is a rare variant of sinonasal undifferentiated carcinoma (SNUC). A 72-year-old man was referred to our hospital with complaints of left facial pain and nasal obstruction. Computed tomography (CI) revealed a tumor 5.5 cm in size in the left nasal cavity. Atypical cells with eosinophilic cytoplasm proliferating as solid nests and exhibiting necrosis were observed and diagnosed as poorly differentiated carcinoma. Carbon ion radiotherapy was performed. Follow-up CI revealed multiple masses in both lungs. Partial resection of the right lung was performed. Proliferating atypical cells with clear-to-eosinophilic cytoplasm were observed and resembled those in the paranasal sinus tumor. Immunohistochemical analysis indicated a metastatic lung tumor derived from the SNUC revealed completely negative SMARCB1 expression in the nuclei of the tumor cells. SdSNC is difficult to diagnose. However, molecular targeted therapy may be useful. Thus, it is necessary and important to recognize this rare cancer accurately.
The energy security and insecurity of Bangladesh: a critical analysis
Authors: Siddiky Chowdhury Ishrak Ahmed 
Published: April 2021

Abstract
The last five years have seen Bangladesh making good progress in adding various energy mixes to its supply of energy for domestic consumption. The GDP of the country grew at 8 per cent in the year before the pandemic. This was possible due to certain policy-level decisions by the government to increase the production of energy, implementing short-term projects for electricity generation and the flexibility of the industrial sector. The country now has a plan to increase its energy security and lower its reliability on imported energy. The purpose of this article is to discuss and analyse the various policies and the challenges that the government faces in order to ensure the country’s energy security.
The bacterial biocontrol agent Paenibacillus alvei K165 confers inherited resistance to Verticillium dahliae
Authors: Gkizi Danai , González Gil Anna , Pardal Alonso J , Piquerez Sophie J M , Sergaki Chrysi , Ntoukakis Vardis , Tjamos Sotirios E 
Published: April 2021

Abstract
The biocontrol agent Paenibacillus alvei K165 was previously shown to protect Arabidopsis thaliana plants against Verticillium dahliae. Here we show that K165 also confers inherited immune resistance to V. dahliae. By performing a histone acetyltransferases mutant screen, ChIP assays, and transcriptomic experiments, we were able to show that histone acetylation significantly contributes to the K165 biocontrol activity and establishment of inheritable resistance to V. dahliae. K165 treatment primed the expression of immune-related marker genes and the cinnamyl alcohol dehydrogenase gene CAD3 through the function of histone acetyltransferases. Our results reveal that offspring of plants treated with K165 have primed immunity and enhanced lignification, both contributing towards the K165-mediated inherited immune resistance. Thus, our study paves the way for the use of biocontrol agents for the establishment of inheritable resistance to agronomically important pathogens.
3D distribution models of Ca, Cr, and Fe in a magnetic CP star atmosphere with anisotropic wind
Authors: Alecian G , Stift M J 
Published: April 2021

ABSTRACT
We present time-dependent atomic diffusion calculations for Ca, Cr, and Fe in a stellar magnetic atmosphere including an anisotropic wind. For three different models of mass-loss rates (or wind), we obtain a 3D description of the entire atmosphere. In two of these models, the mass-loss rate varies according to the magnetic field inclination, the assumed field geometry being non-axisymmetric. When the dipolar component of the magnetic field dominates, we find that ring-like abundance structures will be prominent. Spot-like distributions can also exist according to the field geometry. Abundance distributions turn out to be highly sensitive to the mass-loss model. The results are discussed and compared to an observational model of a real chemically peculiar star (θ Aurigae) that features parameters close to those of the model we have adopted for our calculations.
Planes of satellites around Milky Way/M31-mass galaxies in the FIRE simulations and comparisons with the Local Group
Authors: Samuel Jenna , Wetzel Andrew , Chapman Sierra , Tollerud Erik , Hopkins Philip F , Boylan-Kolchin Michael , Bailin Jeremy , Faucher-Giguère Claude-André 
Published: April 2021

ABSTRACT
We examine the prevalence, longevity, and causes of planes of satellite dwarf galaxies, as observed in the Local Group. We use 14 Milky Way/Andromeda-(MW/M31) mass host galaxies from the Feedback In Realistic Environments-2 simulations. We select the 14 most massive satellites by stellar mass within $d_\mathrm{host}\le 300\mathrm{\, kpc}$ of each host and correct for incompleteness from the foreground galactic disc when comparing to the MW. We find that MW-like planes as spatially thin and/or kinematically coherent as observed are uncommon, but they do exist in our simulations. Spatially thin planes occur in 1–2 per cent of snapshots during z = 0−0.2, and kinematically coherent planes occur in 5 per cent of snapshots. These planes are generally transient, surviving for &lt;500 Myr. However, if we select hosts with a Large Magellanic Cloud-like satellite near first pericentre, the fraction of snapshots with MW-like planes increases dramatically to 7–16 per cent, with lifetimes of  0.7–1 Gyr, likely because of group accretion of satellites. We find that M31’s satellite distribution is much more common: M31’s satellites lie within ∼1σ of the simulation median for every plane metric we consider. We find no significant difference in average satellite planarity for isolated hosts versus hosts in LG-like pairs. Baryonic and dark matter-only simulations exhibit similar levels of planarity, even though baryonic subhaloes are less centrally concentrated within their host haloes. We conclude that planes of satellites are not a strong challenge to ΛCDM cosmology.
Non-Kolmogorov turbulence in multiphase intracluster medium driven by cold gas precipitation and AGN jets
Authors: Wang C , Ruszkowski M , Pfrommer C , Oh S Peng , Yang H-Y K 
Published: April 2021

ABSTRACT
Active galactic nuclei (AGNs) feedback is responsible for maintaining plasma in global thermal balance in extended haloes of elliptical galaxies and galaxy clusters. Local thermal instability in the hot gas leads to the formation of precipitating cold gas clouds that feed the central supermassive black holes, thus heating the hot gas and maintaining global thermal equilibrium. We perform 3D magnetohydrodynamical (MHD) simulations of self-regulated AGNs feedback in a Perseus-like galaxy cluster with the aim of understanding the impact of the feedback physics on the turbulence properties of the hot and cold phases of the intracluster medium (ICM). We find that, in general, the cold phase velocity structure function (VSF) is steeper than the prediction from Kolmogorov’s theory. We attribute the physical origin of the steeper slope of the cold phase VSF to the driving of turbulent motions primarily by the gravitational acceleration acting on the ballistic clouds. We demonstrate that, in the pure hydrodynamical case, the precipitating cold filaments may be the dominant agent driving turbulence in the hot ICM. The arguments in favour of this hypothesis are that: (i) the cold phase mass dominates over hot gas mass in the inner cool core; (ii) hot and cold gas velocities are spatially correlated; (iii) both the cold and hot phase velocity distributions are radially biased. We show that, in the MHD case, the turbulence in the ambient hot medium (excluding the jet cone regions) can also be driven by the AGN jets. The driving is then facilitated by enhanced coupling due to magnetic fields of the ambient gas and the AGN jets. In the MHD case, turbulence may thus be driven by a combination of AGN jet stirring and filament motions. We conclude that future observations, including those from high spatial and spectral resolution X-ray missions, may help to constrain self-regulated AGN feedback by quantifying the multitemperature VSF in the ICM.
Opposite roles of transcription elongation factors Spt4/5 and Elf1 in RNA polymerase II transcription through B-form versus non-B DNA structures
Authors: Xu Jun , Chong Jenny , Wang Dong 
Published: April 2021

AbstractTranscription elongation can be affected by numerous types of obstacles, such as nucleosome, pausing sequences, DNA lesions and non-B-form DNA structures. Spt4/5 and Elf1 are conserved transcription elongation factors that promote RNA polymerase II (Pol II) bypass of nucleosome and pausing sequences. Importantly, genetic studies have shown that Spt4/5 plays essential roles in the transcription of expanded nucleotide repeat genes associated with inherited neurological diseases. Here, we investigate the function of Spt4/5 and Elf1 in the transcription elongation of CTG•CAG repeat using an in vitro reconstituted yeast transcription system. We found that Spt4/5 helps Pol II transcribe through the CTG•CAG tract duplex DNA, which is in good agreement with its canonical roles in stimulating transcription elongation. In sharp contrast, surprisingly, we revealed that Spt4/5 greatly inhibits Pol II transcriptional bypass of CTG and CAG slip-out structures. Furthermore, we demonstrated that transcription elongation factor Elf1 individually and cooperatively with Spt4/5 inhibits Pol II bypass of the slip-out structures. This study uncovers the important functional interplays between template DNA structures and the function of transcription elongation factors. This study also expands our understanding of the functions of Spt4/5 and Elf1 in transcriptional processing of trinucleotide repeat DNA.
Early Coagulopathy in Pediatric Traumatic Brain Injury: A Pediatric Acute and Critical Care Medicine Asian Network (PACCMAN) Retrospective Study
Authors: Chong Shu-Ling , Ong Gene Yong-Kwang , Zheng Charles Qishi , Dang Hongxing , Ming Meixiu , Mahmood Maznisah , Chan Lawrence Chi Ngong , Chuah Soo Lin , Lee Olive Pei Ee , Qian Suyun , Fan Lijia , Konoike Yoshihiko , Lee Jan Hau ,   on behalf of the Pediatric Acute and Critical Care Medicine Asian Network (PACCMAN)
Published: April 2021

Abstract

BACKGROUND
Although early coagulopathy increases mortality in adults with traumatic brain injury (TBI), less is known about pediatric TBI.

OBJECTIVE
To describe the prothrombin time (PT), activated partial thromboplastin time (APTT), and platelet levels of children with moderate to severe TBI to identify predictors of early coagulopathy and study the association with clinical outcomes.

METHODS
Using the Pediatric Acute and Critical Care Medicine Asian Network (PACCMAN) TBI retrospective cohort, we identified patients <16 yr old with a Glasgow Coma Scale (GCS) ≤13. We compared PT, APTT, platelets, and outcomes between children with isolated TBI and multiple trauma with TBI. We performed logistic regressions to identify predictors of early coagulopathy and study the association with mortality and poor functional outcomes.

RESULTS
Among 370 children analyzed, 53/370 (14.3%) died and 127/370 (34.3%) had poor functional outcomes. PT was commonly deranged in both isolated TBI (53/173, 30.6%) and multiple trauma (101/197, 51.3%). Predictors for early coagulopathy were young age (adjusted odds ratio [aOR] 0.94, 95% CI 0.88-0.99, P = .023), GCS < 8 (aOR 1.96, 95% CI 1.26-3.06, P = .003), and presence of multiple trauma (aOR 2.21, 95% confidence interval [CI] 1.37-3.60, P = .001). After adjusting for age, gender, GCS, multiple traumas, and presence of intracranial bleed, children with early coagulopathy were more likely to die (aOR 7.56, 95% CI 3.04-23.06, P < .001) and have poor functional outcomes (aOR 2.16, 95% CI 1.26-3.76, P = .006).

CONCLUSION
Early coagulopathy is common and independently associated with death and poor functional outcomes among children with TBI.
Should Health Care Institutions Mandate SARS-CoV-2 Vaccination for Staff?
Authors: Kates Olivia S , Diekema Douglas S , Blumberg Emily A 
Published: April 2021

Abstract
Health care workers have been prioritized for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) vaccination, but vaccine hesitancy among workers may limit uptake. Institutions may wish to consider SARS-CoV-2 vaccine mandates for health care workers, but such proposals raise important ethical questions. Arguments supporting mandates emphasize the proposed favorable balance of harms and benefits for both individuals and communities, as well as moral duties of health care workers and organizations. Arguments in opposition seek to challenge some claims about utility and raise additional concerns about infringement on autonomy, damage to organizational relationships, and injustice. While available SARS-CoV-2 vaccines remain under an experimental designation, mandates may be excessively problematic, but following approval by the Food and Drug Administration mandates may be reconsidered. The authors summarize ethical arguments and practical considerations, concluding that mandates may be ethically permissible in select circumstances.