bluuebunny/crossref_metadata_2025_split huggingface dataset:
Genetic Diagnosis in Hereditary Hemochromatosis: Discovering and Understanding the Biological Relevance of Variants
Authors: Baas Floor S , Rishi Gautam , Swinkels Dorine W , Subramaniam V Nathan 
Published: June 2021

AbstractBackgroundHereditary hemochromatosis (HH) is a genetic disease, leading to iron accumulation and possible organ damage. Patients are usually homozygous for p. Cys282Tyr in the homeostatic iron regulator gene but may have mutations in other genes involved in the regulation of iron. Next-generation sequencing is increasingly being utilized for the diagnosis of patients, leading to the discovery of novel genetic variants. The clinical significance of these variants is often unknown.ContentDetermining the pathogenicity of such variants of unknown significance is important for diagnostics and genetic counseling. Predictions can be made using in silico computational tools and population data, but additional evidence is required for a conclusive pathogenicity classification. Genetic disease models, such as in vitro models using cellular overexpression, induced pluripotent stem cells or organoids, and in vivo models using mice or zebrafish all have their own challenges and opportunities when used to model HH and other iron disorders. Recent developments in gene-editing technologies are transforming the field of genetic disease modeling.SummaryIn summary, this review addresses methods and developments regarding the discovery and classification of genetic variants, from in silico tools to in vitro and in vivo models, and presents them in the context of HH. It also explores recent gene-editing developments and how they can be applied to the discussed models of genetic disease.
Tunicate Swarm Magnetic Optimization With Deep Convolution Neural Network For Collaborative Filter Recommendation
Authors: Gupta Shefali , Goel Ankit , Dave Dr Meenu 
Published: June 2021

Abstract
Collaborative filtering (CF) is a well-known and eminent recommendation technique to predict the preference of new users by revealing the structures of historical records of the examined users. Even though CF is effectively adapted in several commercial areas, many limitations still exist, particularly in the sparsity of rating data that raises many issues. This paper devises a novel deep learning strategy for CF to recognize user preferences. Here, black hole entropic fuzzy clustering (BHEFC) is devised for clustering item sequences to form groups with similar item sequences. Moreover, cluster centroids are optimized using the tunicate swarm magnetic optimization algorithm (TSMOA), which is devised by combining tunicate swarm algorithm and magnetic optimization algorithm. After grouping similar items together, the group matching is performed based on a deep convolutional neural network (Deep CNN). Subsequently, the visitor sequence and query sequence are compared using Jaro–Winkler distance, which contributes to the best visitor sequence. From this best visitor sequence, the recommended product is acquired. The proposed TSMOA–BHEFC and Deep CNN outperformed other methods with minimal mean absolute error of 0.200, mean absolute percentage error of 0.198 and root mean square error of 0.447, respectively.
Device-related complications after Impella mechanical circulatory support implantation: an IMP-IT observational multicentre registry substudy
Authors: Ancona Marco B , Montorfano Matteo , Masiero Giulia , Burzotta Francesco , Briguori Carlo , Pagnesi Matteo , Pazzanese Vittorio , Trani Carlo , Piva Tommaso , De Marco Federico , Di Biasi Maurizio , Pagnotta Paolo , Casu Gavino , Garbo Roberto , Preti Gerlando , Nicolini Elisa , Sclafani Rocco , Colonna Giuseppe , Mojoli Marco , Siviglia Massimo , Denurra Cristiana , Caprioglio Francesco , Scandroglio Anna Mara , Tarantini Giuseppe , Chieffo Alaide 
Published: June 2021

Abstract

Aims
To report the incidence, the predictors and clinical impact of device-related complications (DRCs) in the IMP-IT (IMPella Mechanical Circulatory Support Device in Italy) registry. Impella is percutaneous left ventricular assist devices, which provides mechanical circulatory support both in cardiogenic shock (CS) and high-risk percutaneous coronary intervention (HR-PCI). The IMP-IT registry is a multicentre registry evaluating the trends in use and clinical outcomes of Impella in Italy.

Methods and results
A total of 406 patients have been included in this registry: 56.4% in the setting of CS, while 43.6% patients in the setting of HR-PCI. DRCs were defined as a composite endpoint of access-site bleeding, limb ischaemia, vascular complication requiring treatment, haemolysis, aortic injury, and left ventricular perforation. DRC incidence in the overall population was 25.6%, with significantly higher rate in the CS (37.1%) than in the HR-PCI (10.7%) group. The most frequent complication was haemolysis (11.8%), which occurred almost exclusively in CS population. Access-site bleeding was observed in 9.6% of the overall population, with no significant difference between the two groups. Limb ischaemia was observed in 8.3% of the overall population, with significantly higher rate in the CS group. CS and right ventricular dysfunction appear as the strongest independent predictors of DRC. One-year mortality in patients with DRC appears higher than in patients with no DRC. However, DRC was not confirmed as an independent predictor of 1-year mortality at multivariate analysis.

Conclusion
In the IMP-IT registry, the rate of DRC was 25.6%, with CS being the strongest independent predictor. DRC was not found as an independent predictor of 1-year mortality.
Staphylococcus aureusmembrane-damaging activities of four phenolics
Authors: Bouarab Lynda , Degraeve Pascal , Bouajila Jalloul , Cottaz Amandine , Jbilou Fouzia , Joly Catherine , Oulahal Nadia 
Published: June 2021

AbstractThe membrane-damaging activities of four phenolics chosen for their bactericidal activity against Staphylococcus aureus CNRZ3 were investigated: 5,7-dihydroxy-4-phenylcoumarin (DHPC), 5,8-dihydroxy-1,4-naphthoquinone (DHNQ), epigallocatechin gallate (EGCG) and isobutyl 4-hydroxybenzoate (IBHB). Staphylococcus aureus CNRZ3 cells, as well as model liposomes mimicking its membrane phospholipids composition, were treated with each phenolic at its minimal bactericidal concentration. Membrane integrity, intracellular pH and intracellular esterase activity were examined by flow cytometric analysis of S. aureus cells stained with propidium iodide and SYTO® 9, 2′,7′-bis(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester, and 5(6)-carboxyfluorescein diacetate, respectively. While intracellular pH was affected by the foyr phenolics, only DHNQ and to a lesser extent EGCG, caused a loss of membrane integrity. Flow cytometric analysis of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and DPPC/POPG (2-oleoyl-1-palmitoyl-sn-glycero-3-phosphoglycerol) liposomes stained with Coumarin 6 (which penetrates the lipid bilayer) or 5-N(octadecanoyl)-amino-fluorescein (which binds to the liposome shell) suggested that only EGCG and DHNQ penetrated the bilayer of phospholipids of liposomes. Taken together, these findings support the hypothesis that EGCG and DHNQ bactericidal activity results from their accumulation in the phospholipid bilayer of S. aureus cells membrane causing its disruption.
The herbicide cycloxydim is an effective alternative to propyzamide or glyphosate for the control of the forest grass weeds Molinia caerulea, Calamagrostis epigejos, Deschampsia flexuosa and Holcus lanatus
Authors: Willoughby Ian H , Forster Jack 
Published: June 2021

Abstract
We tested the efficacy of a range of herbicides to see if they might be potential substitutes for the use of propyzamide or glyphosate to control grass weed species that, if left unchecked, often outcompete recently planted trees in UK forests.
Applications of 0.45kg a.i. ha−1 cycloxydim in late April–early May gave good control of wood small reed (Calamagrostis epigejos (L.) Roth), Yorkshire fog (Holcus lanatus L.), and wavy hair grass (Deschampsia flexuosa (L.) Trin.)) for at least 12 weeks after application. When coupled with a sequential application of 0.15kg a.i. ha−1 propaquizafop made 5 weeks later, purple moor grass (Molinia caerulea (L.) Moench) was controlled for at least 21 weeks.
Sequential applications of 0.45 kg a.i. ha−1 cycloxydim in October, then 0.45 kg a.i. ha−1 cycloxydim in late April, was the most effective treatment for minimizing competition from purple moor grass, wood small reed or wavy hair grass and equally effective as propyzamide on Yorkshire fog.
The herbicides carbetamide, clethodim, clodinafop-propargyl and fenoxaprop-p-ethyl were not as effective as cycloxydim.
We conclude that cycloxydim is effective on a broader range of grass weeds than previously thought. Cycloxydim and propaquizafop are both highly selective and unlike the broad-spectrum herbicide glyphosate will not harm young trees, even if they are oversprayed when in active growth. If applied at the correct time of year, cycloxydim is likely to be as effective as glyphosate on most grasses, and for wood small reed, purple moor grass and wavy hair grass considerably more effective than propyzamide. A sequential application of cycloxydim or possibly propaquizafop in the autumn, followed by a second spray in late April of the following spring, is likely to be the most effective means of maximizing tree growth and survival in grass dominated new planting or restock sites in UK forests.
Extending the Analysis of Functional Ability Trajectories to Unexplored National Contexts: The Case of Chile
Authors: Madero-Cabib Ignacio , Villalobos Dintrans Pablo , Browne Salas Jorge 
Published: June 2021

Abstract

Objectives
Despite the enormous advances in the field, most evidence about functional ability trajectories in old age comes from studies conducted in developed and high-income countries. This research aims to build on these previous advances to examine functional ability trajectories in Chile.

Method
Drawing on a robust, publicly available 15-year panel data set (2004–2018), and using sequence analysis, we examine functional ability trajectories types among 4 age groups (people aged 46–50, 51–55, 56–60, and 61–64 at baseline). Then, we analyze trajectories’ dynamics looking at intraindividual health-declining and health-recovery transitions between functional ability statuses, within each trajectory type. Finally, we assess how multiple baseline individual characteristics predict the likelihood of following a functional ability trajectory type, using multinomial regression models.

Results
Across all age groups, an important fraction (between 26% and 50%) reports stable healthy trajectories, and between 10% and 20% follow equivocal-declining trajectories (i.e., exhibiting both health-declining and health-recovery intraindividual transitions), suggesting that age might not be the main source of heterogeneity in functional ability trajectories. Overall, women, lower educated people, nonworking individuals, and people with a higher burden of chronic conditions at baseline are more prevalent among health-declining trajectory types; however, these results are not constant across the age groups analyzed.

Discussion
This nationally focused study reinforces the feasibility and usefulness of an in-depth analysis of functional ability trajectories in old age. The study findings can be crucial to define different prevention strategies according to the functional ability path that an individual might follow, especially in countries like Chile that currently navigate the challenges of population aging.
Assessing Age Inclusivity in Higher Education: Introducing the Age-Friendly Inventory and Campus Climate Survey
Authors: Silverstein Nina M , Whitbourne Susan K , Bowen Lauren M , Montepare Joann M , Jansen Taylor , Beaulieu Celeste , Prasad Anyah 
Published: June 2021

AbstractBackground and ObjectivesThis study introduces a theoretical framework for assessing age inclusivity in higher education environments and describes the Age-Friendly Inventory and Campus Climate Survey (ICCS). The ICCS measures age-friendly campus practices as reported by administrators, perceptions of age-friendliness by campus constituents, and the fit between objective practices and subjective perceptions as an overall indicator of age inclusivity.Research Design and MethodsThe ICCS was administered at a public university in the northeastern United States. Administrators completed the Inventory of potential age-friendly campus practices associated with their units. Campus constituents (n = 688) completed the online Campus Climate Survey to assess subjective awareness of these practices, perceived age-friendliness, and personal beliefs about age inclusivity.ResultsThe Inventory yielded a score of 66% of potential age-friendly practices in place as reported by administrators. The Campus Climate Survey showed low overall perceptions of age-friendliness and varied beliefs about age inclusivity on campus. Fit was measured by comparing the Inventory practices and Campus Climate Survey awareness of the existence of 47 of 73 potential practices. Convergence on this campus showed an awareness of 36% of age-friendly practices.Discussion and ImplicationsBased on the proposed theoretical framework for age inclusivity, the ICCS offers a way of assessing the age-friendliness of the objective environment across campus functions, the subjective environment across campus constituents, and the fit between subjective and objective environments on campuses. The ICCS will help higher education institutions identify strengths and challenges for advancing age inclusivity.
Self-Reported Behavioral Symptoms of People With Dementia: A Pilot Study Examining Individuals’ Perceived Illness Experience
Authors: Minyo Morgan J , Judge Katherine S 
Published: June 2021

Abstract

Background and Objectives
Persons with dementia experience behavioral symptoms, such as agitation and repeating questions, which have been reported as one of the most burdensome and stressful aspects of providing care by dementia caregivers. However, no published studies have assessed the subjective experience of behavioral symptoms and distress from people with dementia.

Research Design and Methods
The current pilot study examined the feasibility of people with dementia providing self-reported behaviors and behavioral-related distress. Data from a larger, ongoing research study was used consisting of people with mild to moderate dementia (n = 12) residing in a long-term memory care facility.

Results
Participants were able to provide reliable (α = 0.91) self-reported data concerning their own behaviors and behavioral-related distress with variability among responses. The most frequently self-reported behaviors included agitation (66.7%) and complaining/criticizing things (58.3%) while the least-reported behaviors were refusing to be left alone (8.3%) and yelling/swearing (8.3%). The highest behavioral distress reported was agitation (58.3%) while the least was wandering (8.3%).

Discussion and Implications
Understanding the subjective, perceived experience of people with dementia provides valuable information on the illness experience. Additional research is needed to examine the role and impact of self-reported behaviors and the resulting behavioral-related distress on outcomes of well-being. Subjective reports of behavioral-related distress could predict well-being, above and beyond that of traditional objective measures, creating the potential for novel nonpharmacological intervention development for people with dementia.
One Health and reconciliation: media portrayals of dogs and Indigenous communities in Canada
Authors: Fraser-Celin Valli-Laurente , Rock Melanie J 
Published: June 2021

Summary
This qualitative media analysis explores how the Canadian Broadcasting Corporation (CBC) portrayed ‘dog problems’ and their solutions in Indigenous communities in Canada from 2008 through 2018. We apply a One Health framework to demonstrate how human, animal, and the socio-environmental health are interconnected, which aligns more explicitly with Indigenous worldviews. Through this analysis, we respond to the Truth and Reconciliation Commission of Canada (TRC) Calls to Action, specifically Action 19 (health inequity) and Action 84 (media). We found that the CBC portrayed dogs as “strays” and focused mainly on the removal of dogs, whether rehoming by animal rescue groups or through culling, and that rescue groups were portrayed as ‘animal lovers’. Meanwhile, journalists sometimes mentioned the lack of policies to support community-driven dog population control and veterinary services, but these policy deficits did not receive emphasis. The CBC coverage did not highlight systemic injustices that can impact dog health and welfare in Indigenous communities. This media analysis outlines ways forward for reconciliation with Indigenous communities when the media reports on dogs; we recommend journalists (i) focus on lack of veterinary services in communities and the impacts rather than the removal of dogs, (ii) discuss broader systemic structures and policies that limit access to veterinary services in Indigenous communities and (iii) how such resource constraints impact human and animal health.
Intergenerational change in anthropometry of children and adolescents in the New Delhi Birth Cohort
Authors: Sinha Sikha , Shah Dheeraj , Osmond Clive , Fall Caroline H D , Bhargava Santosh K , Sachdev Harshpal Singh 
Published: June 2021

Abstract

Background
A comparison of the anthropometry of children and adolescents with that of their parents at the same age may provide a more precise measure of intergenerational changes in linear growth and body mass index (BMI).

Methods
New Delhi Birth Cohort participants (F1), born between 1969 and 1972, were followed up for anthropometry at birth and at 6-monthly intervals until 21 years of age. At variable intervals 1447 children, aged 0–19 years (F2) and born to 818 F1 participants, were measured (weight and height), providing 2236 sets of anthropometries. Intergenerational changes (F2-F1) in height and BMI [absolute and standard deviation (SD) units] were computed by comparing children with their parents at corresponding ages.

Results
F2 children were taller (P &lt; 0.001) than their parents at corresponding ages; the increase {mean [95% confidence interval (CI)] World Health Organization SD units} was 0.97 (0.83, 1.11), 1.21 (1.10, 1.32), 1.09 (0.98, 1.19), 1.10 (1.00, 1.21) and 0.75 (0.65, 0.85) for age categories of 0–5, 5–7.5, 7.5–10, 10–12.5 and &gt;12.5 years, respectively. In absolute terms, this increase ranged from 3.5 cm (0–5-year-olds) to 7.5 cm (10–12.5-year-olds). The corresponding increases in BMI SD scores were 0.32 (0.18, 0.47), 0.60 (0.45, 0.75), 1.13 (0.99, 1.27), 1.30 (1.15, 1.45) and 1.00 (0.85, 1.15), respectively. The absolute BMI increase ranged from 1–3 kg/m2 at &gt;5 years age to ∼3 kg/m2 at &gt;10-years of age. The intergenerational increases were comparable in both sexes, but were greater in children born and measured later. A positive change in socioeconomic status was associated with an increase in height across the generations.

Conclusions
Children and adolescents, throughout the ages 0–19 years, have become considerably taller and have a higher BMI than their parents at corresponding ages in an urban middle-class Indian population undergoing socioeconomic improvements.
Using sustainable development indicators in developing Saudi cities—case study: Makkah city
Authors: Helmi Mansour , Hegazy Ibrahim , Qurnfulah Emad , Maddah Rahif , Ibrahim Hossam Samir 
Published: June 2021

Abstract
The Kingdom of Saudi Arabia has achieved a rate of 69% in development sustainability level and ranked 101st out of 157 countries participating in the United Nations (UN) Convention of Sustainable Development Achievement. This shows a weakness in the extent of achieving sustainable development in the cities of the Kingdom of Saudi Arabia. Therefore, Makkah city, as one of the most significant cities in the Kingdom of Saudi Arabia, was selected to measure to what extent it achieves sustainable development. The study sought to identify the indicators through which the extent of sustainable development achievement can be measured in Makkah. The values of indicators in Makkah were determined with reference to the accredited authorities in the Kingdom of Saudi Arabia, such as the General Authority for Statistics and the establishment of the Makkah Comprehensive Plan. In addition, the quality-of-life program document was reviewed and links between the sustainable development indicators and livability indicators included in the quality-of-life document were considered. Then, a gap analysis was identified to find out the difference between the status in Makkah and the standard established by the UN and those included in the quality-of-life document. Finally, the study revealed the extent of achieving sustainable development in Makkah, which reached a score of 77.5%. The weaknesses of the city were determined in comparison to the established standards. Initiations and several programs were proposed for the purpose of bridging the gap identified in the study.
Analysis of Neutralizing Antibodies as a Correlate of Instantaneous Risk of Hospitalized Dengue in Placebo Recipients of Dengue Vaccine Efficacy Trials
Authors: Huang Ying , Williamson Brian D , Moodie Zoe , Carpp Lindsay N , Chambonneau Laurent , DiazGranados Carlos A , Gilbert Peter B 
Published: June 2021

Abstract

Background
In the CYD14 (NCT01373281) and CYD15 (NCT01374516) dengue vaccine efficacy trials, month 13 neutralizing antibody (nAb) titers correlated inversely with risk of symptomatic, virologically confirmed dengue (VCD) between month 13 (1 month after final dose) and month 25. We assessed nAb titer as a correlate of instantaneous risk of hospitalized VCD (HVCD), for which participants were continually surveilled for 72 months.

Methods
Using longitudinal nAb titers from the per-protocol immunogenicity subsets, we estimated hazard ratios (HRs) of HVCD by current nAb titer value for 3 correlate/endpoint pairs: average titer across all 4 serotypes/HVCD of any serotype (HVCD-Any), serotype-specific titer/homologous HVCD, and serotype-specific titer/heterologous HVCD.

Results
Baseline-seropositive placebo recipients with higher average titer had lower instantaneous risk of HVCD-Any in 2- to 16-year-olds and in 9- to 16-year-olds (HR, 0.26 or 0.15 per 10-fold increase in average titer by 2 methods [95% confidence interval {CI}, .14–.45 and .07–.34, respectively]) pooled across both trials. Results were similar for homologous HVCD. There was evidence suggesting increased HVCD-Any risk in participants with low average titer (1:10 to 1:100) compared to seronegative participants (HR, 1.85 [95% CI, .93–3.68]).

Conclusions
Natural infection–induced nAbs were inversely associated with hospitalized dengue, upon exceeding a relatively low threshold.