bluuebunny/crossref_metadata_2025_split huggingface dataset:
Highly functional Cellular Immunity in SARS-CoV-2 Non-Seroconvertors is associated with immune protection
Authors: Kilpeläinen Athina , Jimenez-Moyano Esther , Blanch-Lombarte Oscar , Ouchi Dan , Peña Ruth , Quirant-Sanchez Bibiana , Chamorro Anna , Blanco Ignacio , Martínez-Caceres Eva , Paredes Roger , Mateu Lourdes , Carrillo Jorge , Blanco Julià , Brander Christian , Massanella Marta , Clotet Bonaventura , Prado Julia G. 
Published: May 2021

ABSTRACTThe role of T cells in the control of SARS-CoV-2 infection has been underestimated in favor of neutralizing antibodies. However, cellular immunity is essential for long-term viral control and protection from disease severity. To understand T-cell immunity in the absence of antibody generation we focused on a group of SARS-CoV-2 Non-Seroconvertors (NSC) recovered from infection. We performed an immune comparative analysis of SARS-CoV-2 infected individuals stratified by the absence or presence of seroconversion and disease severity. We report high levels of total naïve and low effector CD8+ T cells in NSC. Moreover, polyfunctional Nucleocapsid (NP)-specific CD8+ T-cell responses, as well as reduced levels of T-cell activation monitored by PD-1 and activation-induced markers, were distinctive immunological traits in NSC. Longitudinal data support the stability of the NSC phenotype over three months. Our results implicate highly functional SARS-CoV-2 Spike and NP T-cell responses with low immune activation in protection from disease severity in the absence of seroconversion.SUMMARYTo understand SARS-CoV-2 specific T-cell immunity in the absence of seroconversion, we characterized immunological features of Non-Seroconvertors recovered from infection. Highly functional specific T-cell responses and low immune activation were determinants of immune protection from severe disease.
Gene by environment interaction mouse model reveals a functional role for 5-hydroxymethylcytosine in neurodevelopmental disorders
Authors: Papale Ligia A. , Madrid Andy , Zhang Qi , Chen Kailei , Sak Lara , Keleş Sündüz , Alisch Reid S. 
Published: May 2021

AbstractMouse knockouts ofCntnap2exhibit altered neurodevelopmental behavior and a genome-wide disruption of 5-hydroxymethylcytosine (5hmC). Here we examined whether adultCntnap2heterozygous mice (Cntnap2+/-, lacking behavioral or neuropathological abnormalities) subjected to a prenatal stress would have disruptions in brain 5hmC levels and exhibit altered behaviors similar to the knockout mice. Adult prenatally stressedCntnap2+/-female mice showed repetitive behaviors and altered sociability, similar to the homozygote phenotype. Genomic profiling revealed disruptions in hippocampal and striatal 5hmC levels that were correlated to altered transcript levels of genes linked to these phenotypes (e.g., Reln,Dst,TrioandEpha5). Chromatin-immunoprecipitation coupled with high-throughput sequencing and hippocampal nuclear lysate pull-down data indicated that 5hmC abundance alters the binding of the transcription factor CLOCK near the promoters of differentially expressed genes (e.g., Palld,Gigyf1, andFry), providing a mechanistic role for 5hmC (disruption of transcription factor binding) in gene regulation of developmentally important genes.
A New Assay Capturing Chromosome Fusions Shows a Protection Trade-off at Telomeres and NHEJ Vulnerability to Low Density Ionising Radiation
Authors: Pobiega Sabrina , Alibert Olivier , Marcand Stéphane 
Published: May 2021

AbstractChromosome fusions threaten genome integrity and promote cancer by engaging catastrophic mutational processes, namely chromosome breakage-fusion-bridge cycles and chromothripsis. Chromosome fusions are frequent in cells incurring telomere dysfunctions or those exposed to DNA breakage. Their occurrence and therefore their contribution to genome instability in unchallenged cells is unknown. To address this issue, we constructed a genetic assay able to capture and quantify rare chromosome fusions in budding yeast. This chromosome fusion capture assay (CFC) relies on the controlled inactivation of one centromere to rescue unstable dicentric chromosome fusions. It is sensitive enough to quantify the basal rate of end-to-end chromosome fusions occurring in wild-type cells. These fusions depend on canonical nonhomologous end-joining (NHEJ). Our results show that chromosome end protection results from a trade-off at telomeres between positive effectors (Rif2, Sir4, telomerase) and a negative effector partially antagonizing them (Rif1). The CFC assay also captures NHEJ-dependent chromosome fusions induced by ionising radiation. It provides evidence for chromosomal rearrangements stemming from a single photon-matter interaction.
Transcriptome analysis of heterogeneity in mouse model of metastatic breast cancer
Authors: Ionkina Anastasia A. , Balderrama-Gutierrez Gabriela , Ibanez Kristian J. , Phan Steve Huy D. , Cortez Angelique N. , Mortazavi Ali , Prescher Jennifer A. 
Published: May 2021

AbstractCancer metastasis is a complex process involving the spread of malignant cells from a primary tumor to distal organs. Understanding this cascade at a mechanistic level could provide critical new insights into the disease and potentially reveal new avenues for treatment. Transcriptome profiling of spontaneous cancer models is an attractive method to examine the dynamic changes accompanying tumor cell spread. However, such studies are often complicated by the underlying heterogeneity of the cell types involved. Here we performed a comprehensive analysis of tumor heterogeneity and organ tropism during breast cancer metastasis. We created a suite of organ-derived metastatic cell lines from the MMTV-PyMT mouse model. Bulk sequencing analyses uncovered tissue-specific genes across the different metastatic and primary tumor samples. We further investigated intratumoral heterogeneity by performing single-cell RNA-seq. These data revealed transcriptomes that may contribute to sub-clonal evolution during cancer progression. Collectively, the organ-derived cell lines provide a platform for comprehensive studies of metastatic breast cancer progression.
Immunolocalization studies of vimentin and ACE2 on the surface of cells exposed to SARS-CoV-2 Spike proteins
Authors: Lalioti Vasiliki , González-Sanz Silvia , Lois-Bermejo Irene , González-Jiménez Patricia , Viedma-Poyatos Álvaro , Merino Andrea , A. Pajares María , Pérez-Sala Dolores 
Published: May 2021

AbstractThe Spike protein from SARS-CoV-2 mediates docking of the virus onto cells and contributes to viral invasion. Several cellular receptors are involved in SARS-CoV-2 Spike docking at the cell surface, including ACE2 and neuropilin. The intermediate filament protein vimentin has been reported to be present at the surface of certain cells and act as a co-receptor for several viruses; furthermore, its potential involvement in interactions with Spike proteins has been proposed. Here we have explored the binding of Spike protein constructs to several cell types using low-temperature immunofluorescence approaches in live cells, to minimize internalization. Incubation of cells with tagged Spike S or Spike S1 subunit led to discrete dotted patterns at the cell surface, which showed scarce colocalization with a lipid raft marker, but consistent coincidence with ACE2. Under our conditions, vimentin immunoreactivity appeared as spots or patches unevenly distributed at the surface of diverse cell types. Remarkably, several observations including potential antibody internalization and adherence to cells of vimentin-positive structures present in the extracellular medium exposed the complexity of vimentin cell surface immunoreactivity, which requires careful assessment. Notably, overall colocalization of Spike and vimentin signals markedly varied with the cell type and the immunodetection sequence. In turn, vimentin-positive spots moderately colocalized with ACE2; however, a particular enrichment was detected at elongated structures positive for acetylated tubulin, consistent with primary cilia, which also showed Spike binding. Thus, these results suggest that vimentin-ACE2 interaction could occur at selective locations near the cell surface, including ciliated structures, which can act as platforms for SARS-CoV-2 docking.
Does supervisor gender moderate the mediation of job embeddedness between LMX and job satisfaction?
Authors: Stewart Jodie Louise , Wiener Karl Kilian Konrad 
Published: May 2021

PurposeThis paper aims to examine the quality of the relationship between a supervisor and their subordinate, conceptualised as leader member exchange (LMX), and the mediating influence of subordinate’s job embeddedness on job satisfaction. The LMX model considered the four-gender dominant leadership style facets, female – affect and loyalty (communal), and male – contribution and professional respect (agentic). Social role theory was applied to explain societies influence on leadership style. The moderating influence of supervisor gender on the relationship of LMX facets and subordinate embeddedness is investigated.Design/methodology/approachThis cross-sectional survey study of 213 self-selected employed participants investigated the mediation of job embeddedness LMX and job embeddedness and the moderation impact of supervisor gender on this mediation.FindingsJob embeddedness mediated the relationship between all four facets of LMX and job satisfaction. Supervisor gender did not moderate the relationships of the four LMX facets and job embeddedness. These findings highlight the potential impact of a homogeniuos sample in relation to industry type and culture as this may impact on the findings. That is, participants in this study were predominantly females working in female dominant industries.Originality/valueThis study builds on the work of Collinset al.(2014) who examined the moderating impact of subordinate gender on the mediating relationship of job embeddedness on the relationship between LMX facets and job satisfaction. Previously, the gender role of supervisors on this relationship was not explored.
A housing price index with the improvement-value adjusted repeated sales (IVARS) method
Authors: Yiu Chung Yim Edward , Cheung Ka Shing 
Published: May 2021

Purpose
The repeat sales house price index (HPI) has been widely used to measure house price movements on the assumption that the quality of properties does not change over time. This study aims to develop a novel improvement-value adjusted repeat sales (IVARS) HPI to remedy the bias owing to the constant-quality assumption.

Design/methodology/approach
This study compares the performance of the IVARS model with the traditional hedonic price model and the repeat sales model by using half a million repeated sales pairs of housing transactions in the Auckland Region of New Zealand, and by a simulation approach.

Findings
The results demonstrate that using the information on improvement values from mass appraisal can significantly mitigate the time-varying attribute bias. Simulation analysis further reveals that if the improvement work done is not considered, the repeat sales HPI may be overestimated by 2.7% per annum. The more quality enhancement a property has, the more likely it is that the property will be resold.

Practical implications
This novel index may have the potential to enable the inclusion of home condition reporting in property value assessments prior to listing open market sales.

Originality/value
The novel IVARS index can help gauge house price movements with housing quality changes.
Measuring the knowledge and attitudes of physicians towards patients with HIV/AIDS: study of Anatolian group
Authors: Kaya Safak , Arac Esref , Akgul Fethiye , Comoglu Senol , Kaya Sehmuz , Araç Songul , Yildiz Yesim , Buyuktuna Seyit Ali , Kayaaslan Bircan , Parlak Emine , Baysal Birol , Karakecili Faruk , Balik Elif Zelal , Akkoç Ali , Ozdemir Kevser , Kavak Seyhmus , Dogan Suat Ali , Günay Emrah , Karabela Semsi Nur , Cabalak Mehmet , Cag Yasemin , Avci Veli , Durdu Yasemin , Kaya Zehra , Kilic Damla , Yerlikaya Halis , Tarakçı Hüseyin , Mentes Osman , Sağmak Tartar Ayse , Kose Adem , Alakus Omer Faruk , Aktas Ulas , Komek Halil , Aksoz Selcuk 
Published: May 2021

Purpose
This paper aims to determine the knowledge and attitudes of the physicians regarding human immunodeficiency virus (HIV)/acquired immune deficiency syndrome (AIDS), to emphasize that these patients exist and they will exist in the future and to raise awareness so as to prevent that their rights to treatment are revoked.

Design/methodology/approach
The survey was conducted via a link sent through an online system. Random physicians from 81 cities of the country were invited to the survey. The survey has 41 questions regarding knowledge and attitudes in total, including epidemiological information such as age, gender and title.

Findings
A total of 3,107 physicians has voluntarily participated in the study. In total, 2,195 (70.7%) are internal physicians and 912 (29.3%) are surgical physicians among the participant physicians. In total, 1,452 (46.7%) of the participants are specialist physicians, 608 (19.6%) of the participants are practising physician and the rest of it is physician assistants, academicians and dentists, respectively.

Originality/value
In this study, it has been found out that the physicians have a lack of knowledge on HIV/AIDS and they adopt a discriminatory attitude towards HIV-positive persons. HIV-positive patients who are exposed to discrimination and scared of being uncovered refrain from applying to hospitals for treatment, which puts public health into jeopardy due to the high viral load and these patients are faced with difficulties in coping with both medical and emotional load of the disease.
What should you be talking about? The communication pathway to sustainable supply chain contagion
Authors: Mir Saif , Fugate Brian S. , Johnson Jonathan L. , Blessley Misty 
Published: May 2021

PurposeThe purpose of this paper is to understand communication pathways and factors that cause sustainability initiatives to become contagious from downstream to upstream members of a supply chain, which is termed sustainable supply chain contagion (SSCC).Design/methodology/approachThis study takes an inductive, grounded theory approach, while utilizing established theories.FindingsThe decision to implement a sustainability initiative depends on the business case for the organization. Importantly, the findings outline several network and communication factors that overcome the weak business case and, therefore, foster SSCC. Based on these findings, a communication network model of SSCC is outlined. Network factors include the contagion pathways, the role of sustainability and top management teams and communication channels. Communication factors include the alignment of sustainability initiatives with departmental objectives, the articulation of goals and assuring the endurance of a sustainability initiative.Practical implicationsManagers can utilize the proposed model to create conditions that strengthen the business case of a proposed sustainability initiative, thus fostering SSCC. The presented findings reveal different tactics that can assist organizations in communicating sustainability initiatives in a persuasive manner, to permit the proliferation of sustainability across the supply chain.Originality/valueThis research enables a multilevel examination of the factors influencing SSCC.
Influence of organizational life cycle on performance management design in the software industry
Authors: Baiochi Salete Verginia Fontana , Guerino Guilherme Corredato , Leal Gislaine Camila Lapasini , Balancieri Renato , Cotrim Syntia Lemos , Galdamez Edwin Vladimir Cardoza 
Published: May 2021

PurposeThis work investigates the influence of the organizational life cycle (OLC) on the performance management system (PMS) design and the performance of projects in Brazilian industries of software development.Design/methodology/approachThe study employed descriptive statistics and structural equations modeling on the data and information obtained from 115 Brazilian industries of software development with maturity in project management. Data collection was carried out through a survey applied to managers of the organizations mapped and certified by management maturity models Brazilian software process improvement (MPS-BR) or capability maturity model integration (CMMI).FindingsResults revealed that OLC influences the PMS design dimensions (scope, aggregation and opportunity) and project performance. Regarding effects observed on the performance of projects, only the scope dimension's influence was validated by the analyses, and the other dimensions were not significant considering the investigated sample.Originality/valueThe paper shows evidence of how OLC can influence PMS design and project performance. The analyses can help managers to improve their actions in the PMS design, increasing confidence and generating more robust and sustainable results in the business model.
Supply chain quality management impact on organization performance: results from an international survey
Authors: Fernandes Ana Cristina , Vilhena Estela , Oliveira Rui , Sampaio Paulo , Carvalho Maria Sameiro 
Published: May 2021

PurposeIn this paper, Supply Chain Quality Management dimensions are empirically tested in order to understand their impact on the organization performance based on the Balanced Scorecard perspectives.Design/methodology/approachIn order to validate the theoretical model proposed, an empirical study was carried out, supported by a large-scale questionnaire and statistical analysis.FindingsResults show that all the Supply Chain Quality Management dimensions have a significant positive correlation in the four Balanced Scorecard performance perspectives. Product/service quality and quality culture dimensions were the ones that presented the highest average scores. No significant differences were detected in any dimension for the different regions considered in this study.Originality/valueThe present research can help companies to achieve a better performance in the analyzed perspectives: customer, financial, internal process, and learning and growth. This work also contributes to the existing body of knowledge on Supply Chain Quality Management, analyzing its impact on organization performance, considering a more embracing perspective.
High death rate of older persons from COVID-19 in Quebec (Canada) long-term care facilities: chronology and analysis
Authors: Beaulieu Marie , Cadieux Genesse Julien , St-Martin Kevin 
Published: May 2021

Purpose
Among the ten Canadian provinces, Quebec has experienced the most significant excess mortality of older persons during COVID-19. This practice paper aims to present the chronology of events leading to this excess mortality in long-term care facilities (LTCFs) and a comprehensive analysis of the phenomenon.

Design/methodology/approach
Documented content from three official sources: daily briefings by the Quebec Premier, a report from the Canadian Armed Forces and a report produced by Royal Society of Canada experts were analysed.

Findings
Two findings emerge: the lack of preparation in LTCFs and a critical shortage of staff. Indeed, the massive transfer of older persons from hospitals to LTCFs, combined with human resources management and a critical shortage of permanent staff before and during the crisis, generates unhealthy living conditions in LTCFs.

Originality/value
To our knowledge, this paper is the first to analyse official Quebec and Canadian statements concerning COVID-19 from the angle of quality of life and protection of older adults in LTCFs.
“CARE” in social media: perceptions of reputation in the healthcare sector
Authors: Chaudhri Vidhi , Oomen Tessa , Pridmore Jason , Joon Alexandra 
Published: May 2021

PurposeGuided by the growing importance of social-mediated organisational communication, this study examines how communication professionals within healthcare organisations perceive and respond to the reputation impacts of social media on the organisation’s reputation. Although the healthcare sector finds itself in the midst of a (continually) transforming landscape characterised by large amounts of digital health (mis)information and an empowered “patient-as-consumer”, little is known about how professionals in this sector understand the changes and respond to them. Moreover, much extant scholarship on the topic is published in specialised health or medical journals and does not explicitly address the communication implications for healthcare organisations.Design/methodology/approachIn-depth semi-structured interviews were conducted with communication professionals responsible for social media across eight hospitals in the Netherlands. The sample included two participants working as communication consultants/social media advisors for healthcare organisations. In all, 15 interviews were conducted.FindingsBuilding on interviewee perspectives, the authors advance the CARE (Control, Access(ability), Responsive(ness) and Engagement) model of social-mediated communication, highlighting the dualistic characteristics of each dimension. This model is built upon a careful analysis of healthcare professional responses. In an always-on environment, understanding and managing the tensions within the authors’ model may be decisive to the reputation implications of social media use.Originality/valueUnderstanding the tensions within each dimension lends a more nuanced perspective on the potential impact(s) of social media as experienced by professionals in the field. In shifting away from a binary, either/or approach, the paper contributes to explicating the complexities of a pervasive phenomenon (i.e. social-mediated communication) and its multifaceted impacts on the healthcare sector.
IoT-BIM-based digital transformation in facilities management: a conceptual model
Authors: Dahanayake Kalani Chamika , Sumanarathna Nipuni 
Published: May 2021

Purpose
This paper aims to explore the opportunities of integrating internet of things (IoT) with building information modelling (BIM) to support the digital transformation of facilities management (FM). In this regard, a conceptual framework is proposed to implement IoT-BIM-based smart FM in buildings.

Design/methodology/approach
A semi-systematic literature review was conducted to examine the opportunities of integrating IoT-BIM-based smart FM.

Findings
BIM models are seldom used during the operations stage, and the comprehensive digital information developed during the design and construction stage is not efficiently used throughout the building’s life cycle. Therefore, this paper suggests that IoT-BIM can be effectively integrated into six FM functions, namely, energy management, operations and maintenance management, space management, FM project management, emergency management and quality management. IoT-BIM provides a beneficial platform for the digital transformation in FM, optimising the effectiveness and efficiency of buildings.

Originality/value
As a recent approach, the integration of BIM with IoT has created a new direction for moving from traditional FM to digitalise smart FM. However, the adaptation of IoT-BIM concept, particularly for FM, is yet to be explored. Hence, this paper contributes to the IoT-BIM research in the FM domain by highlighting six IoT-BIM-based smart FM for digital transformation in FM.