bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effectiveness of liposomal ozonized oil in reducing ocular microbial flora in patients undergoing cataract surgery
Authors: Spadea Leopoldo , Zanotto Elisa , Cavallo Rossana , Campagna Giuseppe , Giannico Maria Ilaria , Costagliola Ciro ,   ELOOM Study Investigators
Published: May 2021

Purpose:
To evaluate the antimicrobial effectiveness of a liposomal ozonized oil solution used as a home therapy in patients undergoing cataract surgery. Antimicrobial efficacy was evaluated as the reduction in the bacterial load of the most common bacteria isolated from cases with endophthalmitis.

Setting:
20 Italian experimental centers of the Effectiveness of Liposomal Ozonized oil on Ocular Microbial flora before cataract surgery study group.

Design:
Interventional, nonrandomized, paired-eye designed, phase 4 clinical study.

Methods:
A total of 174 patients undergoing cataract surgery were divided into 2 groups: the study group (174 eyes) underwent surgery and received an isotonic ophthalmic solution of 0.5% ozonized oil in liposomes plus hypromellose treatment (2 drops 4 times/d), and the control group (174 contralateral eyes) was treated with saline solution. The treatment lasted for 3 days. Subconjunctival swabs were taken from both eyes of each patient at T0 (the day before starting the treatment and 4 days preoperatively) and at T4 (after 3 days of treatment and 10 min preoperatively) and sent to the laboratory within 24 hours of collection for microbiological analysis.

Results:
30% of 696 swabs taken at T0 were sterile. Contaminated swabs had a high prevalence of coagulase-negative staphylococci, including Staphylococcus epidermidis, and more than 60 different bacterial species were isolated. A significant reduction in microbial load was observed after treatment (>90% of the samples). The microbial load in the control group remained unchanged.

Conclusions:
Liposomal ozonized oil reduced the microbial burden after topical administration in a large study population.
Beta 2-adrenergic receptor mediates noradrenergic action to induce cyclic adenosine monophosphate response element-binding protein phosphorylation in satellite glial cells of dorsal root ganglia to regulate visceral hypersensitivity
Authors: Shen Shanwei , Tiwari Namrata , Madar Jonathan , Mehta Parshva , Qiao Liya Y. 
Published: May 2021

Abstract
Sympathoneuronal outflow into dorsal root ganglia (DRG) is suggested to be involved in sympathetically maintained chronic pain, which is mediated by norepinephrine (NE) action on DRG cells. This study combined in vitro and in vivo approaches to identify the cell types of DRG that received NE action and examined cell type–specific expression of adrenergic receptors (ARs) in DRG. Using DRG explants, we identified that NE acted on satellite glial cells (SGCs) to induce the phosphorylation of cAMP response element-binding protein (CREB). Using primarily cultured SGCs, we identified that beta (β)2-adrenergic receptor but not alpha (α)adrenergic receptor nor other βAR isoforms mediated NE-induced CREB phosphorylation and CRE-promoted luciferase transcriptional activity. Using fluorescence in situ hybridization and affinity purification of mRNA from specific cell types, we identified that β2AR was expressed by SGCs but not DRG neurons. We further examined β2AR expression and CREB phosphorylation in vivo in a model of colitis in which sympathetic nerve sprouting in DRG was observed. We found that β2AR expression and CREB phosphorylation were increased in SGCs of thoracolumbar DRG on day 7 after colitis induction. Inhibition but not augmentation of β2AR reduced colitis-induced calcitonin gene-related peptide release into the spinal cord dorsal horn and colonic pain responses to colorectal distention. Prolonged activation of β2AR in naive DRG increased calcitonin gene-related peptide expression in DRG neurons. These findings provide molecular basis of sympathetic modulation of sensory activity and chronic pain that involves β2AR-mediated signaling in SGCs of DRG.
Portal Annular Pancreas With Common Hepatic Artery Trajectory Abnormality
Authors: Tahara Munenori , Harada Takuya , Wakui Yosuke , Mizukami Shoichiro , Kita Kengo , Funakoshi Tohru , Wakayama Kenji , Ishizu Hiroyuki 
Published: May 2021

Abstract
Portal annular pancreas (PAP) is a rare congenital pancreatic anomaly, in which the uncinate process of the pancreas fuses to the body of pancreas behind the portal vein. Here, we report a case of PAP with common hepatic arterial anomaly, which was identified during surgery. A 57-year-old man who had branch type intraductal papillary mucinous neoplasm in the head of the pancreas developed a nodule in the cystic lesion. We planned pylorus preserving pancreaticoduodenectomy. The common hepatic artery from the celiac artery passing behind the portal vein was revealed in preoperative examinations. During surgery, we discovered that the uncinate process of the pancreas was fused with the body of the pancreas behind the portal vein. We divided the pancreas at the anterior and posterior of the portal vein. The main pancreatic duct was present in the anterior pancreatic stump. We performed pancreaticojejunostomy in the anterior stump and closed the posterior stump by interrupted suture. Forty-four surgical cases of PAP have been reported in the English medical literature. There are few previous reports of PAP which involved an arterial anomaly. Clinicians should consider PAP preoperatively to ensure that the surgeon can appropriately plan pancreatic resection to avoid postoperative complications.
Comparison of Treatment Outcomes Between Gemcitabine With Nab-Paclitaxel and Modified FOLFIRINOX for First-Line Chemotherapy in Metastatic and Recurrent Pancreatic Cancer
Authors: Mie Takafumi , Sasaki Takashi , Takeda Tsuyoshi , Fukuda Koshiro , Furukawa Takaaki , Yamada Yuto , Kasuga Akiyoshi , Matsuyama Masato , Ozaka Masato , Sasahira Naoki 
Published: May 2021

Objectives
To compare the treatment outcomes of gemcitabine with nab-paclitaxel (GnP) and modified FOLFIRINOX (mFFX; a combination of fluorouracil, leucovorin, irinotecan, and oxaliplatin) for metastatic pancreatic cancer.

Methods
We retrospectively analyzed consecutive patients with metastatic or recurrent pancreatic cancer treated with GnP or mFFX as the first-line chemotherapy between March 2014 and December 2019 in our hospital. Treatment outcomes were compared using propensity score matching to adjust for age, sex, performance status, carcinoembryonic antigen levels, carbohydrate antigen 19-9 levels, and disease status (metastatic or recurrent).

Results
Five hundred sixty-eight patients were included (GnP/mFFX, 456/112). After propensity score matching, 218 patients were extracted. The median age was around 61 years, and the proportion of performance status 0 was approximately 90%. The median overall survival values were 14.6 and 15.5 months (P = 0.45), and the median progression-free survival was 7.4 months (P = 0.53) for GnP and mFFX, respectively. The disease control rates were higher in the GnP group (82.6% vs 67.9%, P = 0.02). In nonhematologic adverse events, grade 3/4 anorexia and diarrhea occurred significantly more frequently in the mFFX group.

Conclusions
Gemcitabine with nab-paclitaxel had a higher disease control rate and lower rates of severe anorexia and diarrhea in our propensity-matched population.
Adherence to standardized 8-week mindfulness-based interventions among women with breast or gynecological cancer: a scoping review
Authors: Stanic Jelena , Barth Jürgen , Danon Nadia , Bondolfi Guido , Jermann Françoise , Eicher Manuela 
Published: May 2021

Abstract
Participant adherence to standardized 8-week mindfulness-based interventions (MBIs) may be challenging, and adaptations from intervention protocols have been reported in mindfulness research. We conducted a scoping review to determine how women with breast or gynecological cancer adhered to standardized 8-week MBIs delivered in intervention studies. Searches were conducted for articles published till February 2020 in PubMed, Embase, CINAHL EBSCO, PsycINFO Ovid SP, and Cochrane Library Wiley. The following outcomes were investigated: class and silent retreat attendance, intervention completion rate (ICR), adherence to home practice, and reasons for dropping out from an MBI study. Among the 25 included MBI studies, mindfulness-based stress reduction was the most often delivered intervention and mostly women with stage I–III breast cancer were represented. The duration of classes varied from 1.5 to 3.5 hours. Planned home practice varied from 20 to 60 min/day, and silent retreat varied from 4.5 to 8 hours. Due to heterogeneity in the reporting of class attendance, the data could not be pooled. Six studies reported an average class attendance ranging from 5 to 8.2 classes. Overall, intervention completion rate (the proportion who completed all classes) varied from 26.3% to 100%; however, discontinuations were not systematically reported. Home practice time was reported in 20% of the studies and ranged from 17 to 24 min/day. The main reasons for dropping out from an MBI study were health-related problems, organizational challenges, travel distance, and lack of motivation/commitment. About 70% of the studies reported some data on participant adherence, revealing a relatively high overall frequency of class attendance. However, the monitoring and reporting of participant adherence should be improved in future studies to increase our knowledge on the required amount of participant engagement to improve health outcomes and facilitate the implementation of effective interventions on a larger scale.
Social Cognition in Temporal and Frontal Lobe Epilepsy: Systematic review, Meta-analysis, and Clinical Recommendations
Authors: Ziaei Maryam , Arnold Charlotte , Thompson Kate , Reutens David 
Published: May 2021

AbstractObjectiveDespite the importance of social cognitive functions to mental health and social adjustment, assessment of these functions is absent in routine assessment of epilepsy patients. Thus, this review aims to provide a comprehensive overview of the literature on four major aspects of social cognition among temporal and frontal lobe epilepsy, which is a critical step towards designing new interventions.MethodPapers from 1990-2021 were reviewed and examined for inclusion in this study. After the deduplication process, a systematic review and meta-analysis of 44 and 40 articles, respectively, involving 113 people with frontal lobe epilepsy and 1482 people with temporal lobe epilepsy were conducted.ResultsOur results indicated that while patients with frontal or temporal lobe epilepsy have difficulties in all aspects of social cognition relative to non-clinical controls, the effect sizes were larger for theory of mind (g= 0.95), than for emotion recognition (g=0.69) among temporal lobe epilepsy group. The frontal lobe epilepsy group exhibited significantly greater impairment in emotion recognition compared to temporal lobe. Additionally, people with right temporal lobe epilepsy (g= 1.10) performed more poorly than those with a left-sided (g= 0.90) seizure focus, specifically in the theory of mind domain.ConclusionsThese data point to a potentially important difference in the severity of deficits within the emotion recognition and theory of mind abilities depending on the laterlization of seizure side. We also suggest a guide for the assessment of impairments in social cognition that can be integrated into multidisciplinary clinical evaluation for people with epilepsy
Clinical Significance of Circulating Tumor Cells in Unresectable Pancreatic Ductal Adenocarcinomas
Authors: Kim Hyemin , Heo Chan Mi , Oh Jinmyeong , Lee Eun Mi , Park Juhee , Lee Se-Hoon , Lee Kwang Hyuck , Lee Kyu Taek , Lee Jong Kyun , Cho Yoon-Kyoung , Park Joo Kyung 
Published: May 2021

ABSTRACTBackgroundCirculating tumour cells (CTCs) have emerged as liquid biopsy biomarker providing non-invasive assessment of cancer progression and biology. We investigated whether longitudinal analysis of CTCs could monitor disease progression, response to chemotherapy, and survival in patients with unresectable pancreatic ductal adenocarcinoma (PDAC).MethodsCTCs were isolated using a centrifugal microfluidic disc from serially collected peripheral blood with clinical assessments. CTCs were enumerated with immunostaining against Epithelial cell adhesion molecule, Cytokeratin, Plectin-1 and CD45.ResultsCTCs were detected in 91.7% of 52 patients with unresectable PDAC at the time of diagnosis. CTC numbers were not statistically different across tumour sizes, stages and metastatic sites. The absolute CTC counts after chemotherapy was inversely related to survival, and the decreased number of CTCs after the first cycle of chemotherapy was significantly associated with longer survival.ConclusionsIdentifying CTCs and monitoring CTC changes after chemotherapy could be a useful prognostic marker for survivals in patients with unresectable PDACs.FundingThis work was supported by a grant from SK Chemical Research Fund of the Korean Society of Gastroenterology (Grant No.800-20130378) and a grant from Korean Gastroenterology Fund for Future Development. This study was granted by the Korean Health Technology R&D Project, Ministry of Health & Welfare funded by the Korean Government (Grant No. HI12C1845), and work by Y.K.Cho was partially supported by IBS-R020-D1 funded by the Korean Government. This research was supported by Collaborative Genome Program for Fostering New Post-Genome industry through the National Research Foundation (NRF) funded by the Korean government (MSIT) (Grant No. NRF-2017M3C9A5031002), and also supported by National Research Foundation (NRF) grant funded by the Korean government (MSIT) (Grant No. 2019R1C1C1008646).Clinical Trial NumberClinicalTrials.gov Identifier No. NCT02934984
Risk factors at birth, in childhood and adolescence for the onset of musculoskeletal chronic pain in adulthood: a scoping review
Authors: Michele Margelli , Gianelli Alice , Borghi Lucia , Zanchetta Federico , Salvioli Stefano , Filippo Maselli 
Published: May 2021

AbstractBackgroundLiterature already confirmed that chronic pain is not only correlated to functional deficits, structural problems or neural alterations, but it is also strongly influenced by the presence of psychological and social factors. It was also observed that those factors can have an impact on the subject even after many years: in fact, pain experiences and traumatic events during childhood can lead to changes in stress and pain neural network area and to maladaptive behaviors that concur to develop chronic pain. The main purpose of this review is to identify the bio-psycho-social risk factors in developmental age that could lead to musculoskeletal chronic pain in adulthood.Inclusion criteriaEvery study about risk factors in developmental age (0-18) for the onset of musculoskeletal chronic pain in adulthood will be included. This scoping review will consider studies conducted in any context.MethodsThe proposed scoping review will be conducted in accordance with the Joanna Briggs Institute methodology (JBI) for scoping reviews.The search will be carried out on 6 databases: MEDLINE, Cochrane Central, Scopus, CINHAL, Embase, and PEDro.Selection and data extraction will be conducted by two blind independent researchers and inconsistencies will be resolved by a third reviewer.The results will be presented in a schematic, tabular and descriptive format that will line up with the objectives and scope of the review.ConclusionsThis will be the first scoping review to provide a comprehensive overview of the topic. The results will add meaningful information for clinicians especially during the patient’s assessment. Furthermore, any knowledge gaps of the topic will be identified. The results of this research will be published in a peer-reviewed journal and will be presented at relevant (inter)national scientific events.
Application of Omni-ATAC to Profile Chromatin Accessibility Before and After Ovarian Tissue Cryopreservation
Authors: Shannon Jennifer A. , Sundaresan Aishwarya , Bukulmez Orhan , Jiao Zexu , Capelouto Sarah , Carr Bruce , Banaszynski Laura A. 
Published: May 2021

AbstractOvarian tissue cryopreservation and subsequent autologous transplantation has allowed resumption of endocrine function as well as fertility in certain populations. However, graft function is short-lived due to ischemia and aberrant follicular activation post-transplantation. While many studies have focused on gene expression, we wanted to determine whether cryopreservation itself had a deleterious effect on regulatory elements that might influence transcriptional integrity and graft performance. In this study, we used Omni-ATAC to assess genome-wide chromatin accessibility in primary human follicles before and after cryopreservation. Omni-ATAC from fresh ovarian follicles identified active regulatory elements expected to be functional in oocytes and granulosa cells, and gene ontology was consistent with RNA translation/processing and DNA repair. While promoter accessibility was largely maintained in cryopreserved ovarian follicles, we observed a widespread increase in the number of accessible enhancers. Transcription factor motif analysis and gene ontology suggested that this dysregulation was focused around the epithelial-mesenchymal transition. Indeed, transcription factor binding was noted in major pathways involved in this transition: TGF-β and Wnt signaling. Overall, our work provides the first genomic analysis of active regulatory elements in matched fresh and cryopreserved ovarian follicles as they undergo the process of ovarian tissue cryopreservation. Our data suggest that the process of cryopreservation activates an epithelial-mesenchymal transition state, which may lead to graft burn-out post-transplantation. Optimizing this technique in relation to this transition may therefore be an important step towards improving graft longevity and patient outcomes in fertility preservation.Summary sentenceCryopreservation of ovarian cortical tissue results in activation of differentiation and EMT pathways in follicles, which may explain graft burnout after autotransplantation.
Spatial Association of Respiratory Health with Social and Environmental Factors: Case Study of Cook County, IL
Authors: Lotfata Aynaz , Hohl Alexander 
Published: May 2021

AbstractBackgroundPeople who live with respiratory diseases like asthma are more likely at risk of serious illness. Spatial analytic techniques allow for discovering areas of concern and finding correlates of asthma prevalence.ObjectiveThere is growing interest in disentangling the impacts of socioeconomic and environmental factors on respiratory health, their spatial correlation, and the demographic profile of people at risk of respiratory diseases. It is important to know how people with asthma are geographically distributed and what social and environmental factors correlate with asthma. Thereby, the purpose of the study is to describe socioeconomic factors associated with asthma prevalence in Cook County, IL and to identify the significant risks and the protective factors to control asthma.Data and MethodsData obtained from CDC 2018 SVI, ACS, the City of Chicago Data Portal, HealthData.gov, and ESRI. In this paper, we illustrate the usefulness of geospatial regression analysis in the analysis and presentation of spatially distributed asthma prevalence among the population with disabilities, minorities with the language barrier, nonwhite population, age 17 and younger, and age 65 and older in the census tracts of Cook County, IL where Chicago Metropolitan Area located. In addition, we map the spatial variation of asthma prevalence with variation in the tree canopy, access to medical centers, air quality, and household quality. Lastly, we used bivariate mapping to illustrate the spatial distributions of residential land use and tree covers.ResultsOur findings show a good correlation between asthma and socioeconomic and physical factors including age 17 and younger, age 65 and older, population with disabilities, a minority with the language barrier, tree canopy, access to medical centers, air quality, and household quality. The aged 65 and older, 17 and younger, and people with disabilities are found to have a higher asthma prevalence in areas around the industrial corridors in southeast and west sides of Cook County, IL. Results may guide further decisions in planning for asthma research and intervention, especially for identifying vulnerable areas and people.
Double trouble: having two hosts reduces infection prevalence in vectored trypanosomatids
Authors: Hawra Al-Ghafli , Seth M. Barribeau 
Published: May 2021

AbstractTrypanosomatids are a diverse family of protozoan parasites, some of which cause devastating human and livestock diseases. There are two distinct infection life-cycles in trypanosomatids; some species complete their entire life-cycle in a single host (monoxenous) while others infect two hosts (dixenous). Dixenous trypanosomatids are mostly vectored by insects, and the human trypanosomatid diseases are caused mainly by vectored parasites. While infection prevalence has been described for subsets of hosts and trypanosomatids, little is known about whether monoxenous and dixenous trypanosomatids differ in infection prevalence. Here, we use meta-analyses to synthesise all published evidence of trypanosomatid infection prevalence for the last two decades, encompassing 931 unique host-trypansomatid systems. In examining 581 studies that describe infection prevalence, we find, strikingly, that monoxenous species are two-fold more prevalent than dixenous species across all hosts. We also find that dixenous trypanosomatids have significantly lower infection prevalence in insects than their non-insect hosts. Within monoxenous trypanosomatids, genera infecting bees are most prevalent and infection prevalence does not vary between wild and managed bees. To our knowledge, these results reveal for the first time, a fundamental difference in infection prevalence according to host specificity where vectored species suffer from lower infection prevalence as a result of a ‘jack of all trades, master of none’ style trade-off between the vector and subsequent hosts.