bluuebunny/crossref_metadata_2025_split huggingface dataset:
Knowledge on neonatal jaundice and its associated factors among mothers in northern Ethiopia: a facility-based cross-sectional study
Authors: Demis Asmamaw , Getie Addisu , Wondmieneh Adam , Alemnew Birhan , Gedefaw Getnet 
Published: March 2021

ObjectiveThis study aimed to assess mother’s knowledge on neonatal jaundice (NNJ) and its associated factors in northern Ethiopia.DesignFacility-based cross-sectional study.SettingReferral hospitals in Amhara region, Ethiopia.ParticipantsThe study was done among 380 mothers selected using a systematic random sampling technique at referral hospitals in the Amhara region, northern Ethiopia, from 1 March 2019 to 30 July 2019.Primary outcomeMother’s knowledge on NNJ, modelled using binary logistic regression.Secondary outcomeFactors associated with mother’s knowledge about NNJResultsThis study revealed that the overall mother’s knowledge on NNJ was 39.2%. Having favourable attitude (AOR=2.05, 95% CI: 1.25 to 3.34), having a history of NNJ in previous children (AOR=7.51, 95% CI: 3.12 to 18.05), having a history of NNJ in the current child (AOR=1.97, 95% CI: 1.19 to 3.27), antenatal care (ANC) follow-up (AOR=3.85, 95% CI: 1.24 to 14.55) and resided in the urban area (AOR=2.63, 95% CI: 1.32 to 5.25) were factors significantly associated with mother’s knowledge on NNJ.ConclusionsThis study showed that mothers' knowledge on NNJ was low. The attitude of mothers on NNJ, previous and current child history of NNJ, ANC follow-up and residence were variables that had a significant association with mother’s knowledge on NNJ. Enhancing NNJ educational programmes targeting mothers in raising awareness on the prevention of NNJ in the population are the recommended interventions.
Heterogeneity in COVID-19 patient volume, characteristics and outcomes across US Department of Veterans Affairs facilities: an observational cohort study
Authors: Bravata Dawn M , Myers Laura J , Perkins Anthony J , Keyhani Salomeh , Zhang Ying , Zillich Alan J , Dysangco Andrew , Lindsey Reese , Sharmitha Dev , Myers Jennifer , Austin Charles , Sexson Ali , Arling Greg 
Published: March 2021

ObjectiveStudies describe COVID-19 patient characteristics and outcomes across populations, but reports of variation across healthcare facilities are lacking. The objectives were to examine differences in COVID-19 patient volume and mortality across facilities, and understand whether facility variation in mortality was due primarily to differences in patient versus facility characteristics.DesignObservational cohort study with multilevel mixed effects logistic regression modelling.SettingThe Veterans Health Administration (VA) is the largest healthcare system in the USA.ParticipantsPatients with COVID-19.Main outcomeAll-cause mortality within 45 days after COVID-19 testing (March–May, follow-up through 16 July 2020).ResultsAmong 13 510 patients with COVID-19, 3942 (29.2%) were admitted (2266/3942 (57.5%) ward; 1676/3942 (42.5%) intensive care unit (ICU)) and 679/3942 (17.2%) received mechanical ventilation. Marked heterogeneity was observed across facilities in median age (range: 34.3–83.9 years; facility mean: 64.7, SD 7.2 years); patient volume (range: 1–737 at 160 facilities; facility median: 48.5, IQR 14–105.5); hospital admissions (range: 1–286 at 133 facilities; facility median: 11, IQR 1–26.5); ICU caseload (range: 1–85 at 115 facilities; facility median: 4, IQR 0–12); and mechanical ventilation (range: 1–53 at 90 facilities; facility median: 1, IQR 0–5). Heterogeneity was also observed in facility mortality for all patients with COVID-19 (range: 0%–29.7%; facility median: 8.9%, IQR 2.4%–13.7%); inpatients (range: 0%–100%; facility median: 18.0%, IQR 5.6%–28.6%); ICU patients (range: 0%–100%; facility median: 28.6%, IQR 14.3%–50.0%); and mechanical ventilator patients (range: 0%–100%; facility median: 52.7%, IQR 33.3%–80.6%). The majority of variation in facility mortality was attributable to differences in patient characteristics (eg, age).ConclusionsMarked heterogeneity in COVID-19 patient volume, characteristics and mortality were observed across VA facilities nationwide. Differences in patient characteristics accounted for the majority of explained variation in mortality across sites. Variation in unadjusted COVID-19 mortality across facilities or nations should be considered with caution.
Comparative cohorts of retinopathy of prematurity outcomes of differing oxygen saturation: real-world outcomes
Authors: Choo May May , Grigg John , Barnes Elizabeth H , Khaliddin Nurliza , Kamalden Tengku Ain , Ahmad Kamar Azanna , Choo Yao Mun , Lim Chin Theam , Martin Frank Joseph 
Published: March 2021

ObjectiveAn ongoing third epidemic of retinopathy of prematurity (ROP) is contributed largely by developing nations. We describe a cohort of infants in a single neonatal unit where two limits of oxygen saturation were administered, to show real-world outcomes from trend in neonatology for higher oxygen to improve survival.Methods and analysisThis retrospective, comparative study of prospectively collected data in an ROP screening programme included infants indicated by gestational age ≤32 weeks, birth weight <1501 g, ventilation for 7 days or requiring oxygen >1 month, who underwent dilated fundoscopic examination from age 4 weeks, every 2 weeks until full retinal vascularisation. Infants with ROP were examined weekly and treated where indicated. Data were divided into two epochs. Epoch 1 oxygen saturation targets were [88–92%], epoch 2 targets [90–95% (99%)] with allowance of increase to 20% for several hours after procedures. Outcome measures included development of ROP, treatment, mortality, sepsis and intraventricular haemorrhage.ResultsA total of 651 infants underwent examination between 2003 and 2016. The incidence of ROP in epoch 1 was 29.1% and epoch 2 was 29.3% (p=0.24). ROP progression doubled in epoch 2 (5 vs 11%, p=0.006), proportion of cases treated halved (14% vs 6%, p=0.0005), sepsis was halved (78.5% vs 41.2%, p<0.0001) and intraventricular haemorrhage doubled (20.2% vs 43.8%, p=0.0001) in epoch 2. Mortality was 4% and 0% in epochs 1 and 2, respectively.ConclusionIncidence of ROP did not differ, although ROP cases that worsened doubled with higher oxygen targets. ROP cases requiring treatment decreased, as did sepsis and mortality. Intraventricular haemorrhage cases doubled.
Monotonic and cyclic behaviour of root-reinforced sand
Authors: Karimzadeh Ali Akbar , Leung Anthony Kwan , Hosseinpour Saied , Wu Zhaoyi , Fardad Amini Pedram 
Published: March 2021

Plant roots are known to provide mechanical reinforcement to soils upon shearing and seismic loading. However, the effects of different stress paths on root reinforcement remain unclear. Moreover, whether and how roots provide resistance to soil liquefaction upon cyclic loading have rarely been studied. The objective of this study is to conduct a series of undrained triaxial tests to investigate the monotonic and cyclic behaviour of rooted sand. Roots of vetiver grass (Chrysopogon zizanioides L.), which has been advocated for use in shallow slope stabilisation purposes, were used for testing. The root diameters ranged between 0.3 and 1.5 mm, while the root volume ratios (RVRs) were 0.23%, 0.45%, and 0.67%. It was discovered that the root reinforcement effect was anisotropic and path-dependent. Along the extension path, when the major principal stress was perpendicular to the predominant root orientation, the root-induced increase in soil friction angle was approximately 10°. This increase was much greater than that along the compression path, where the change was minimal. The presence of roots prevented the limited flow failure at some RVRs and cyclic stress ratios (CSRs) (which occurred in the unreinforced sand) and the failure mode of the root-reinforced soil switched to cyclic mobility. The liquefaction resistance was improved with an increase in root volume, and this improvement was more remarkable at higher CSRs.
A comparative evaluation of the nutritional quality of three wild populations of female mitten crabs (Eriocheir sensu stricto) in northern China
Authors: Zhang Dongdong , Jiang Xiaodong , Zu Lu , Zhou Wenquan , Luo Min , Cheng Yongxu , Wu Xugan 
Published: March 2021

Abstract
There are three major wild populations of mitten crab (Eriocheir sensu stricto), Suifenhe River (SF), Tumenjiang River (TM) and Liaohe River (LH) in northern China. However, the information on the nutritional quality of these three populations of crabs is still scarce. Thus, we investigated and compared the tissue indices, total edible yield, proximate composition, fatty acid compositions and amino acid contents of these wild crab populations. We showed that: (1) TM population had the highest meat yield among the three populations; (2) TM recorded the highest moisture and the lowest total lipid content in ovary and muscle; (3) a higher level of total n-3 poly-unsaturated fatty acids was found in TM, except for the ovary in SF; (4) the three populations had similar essential amino acids scores. In conclusion, Eriocheir s.s. from these three populations all showed a high nutritional quality suitable for humans, especially the TM, which contains more eicosapentaenoic acid and docosahexaenoic acid.
Infrasound Generation by Meteorological Fronts and Its Propagation in the Atmosphere
Authors: Chunchuzov I. P. , Kulichkov S. N. , Popov O. E. , Perepelkin V. G. 
Published: March 2021

AbstractInfrasound parameters (amplitudes, coherences, grazing angles, azimuths, and horizontal phase speeds) derived during the passage of warm and cold fronts through the networks of microbarometers in the cities of Dubna and Moscow are presented. The significant differences observed in the temporal variations of the parameters of infrasound from warm and cold fronts are discussed. Such differences must be taken into account when detecting infrasound precursors of atmospheric storms. A possible mechanism for the generation of infrasound by the turbulent airstream flowing around the geometric irregularities of the meteorological front is proposed. The observed effect of internal gravity waves on the parameters of infrasound and its frequency spectrum is explained.
Evaluating and Optimizing Surface Soil Moisture Drydowns in the ORCHIDEE Land Surface Model at In Situ Locations
Authors: Raoult Nina , Ottlé Catherine , Peylin Philippe , Bastrikov Vladislav , Maugis Pascal 
Published: March 2021

AbstractThe rate at which land surface soils dry following rain events is an important feature of terrestrial models. It determines, for example, the water availability for vegetation, the occurrences of droughts, and the surface heat exchanges. As such, surface soil moisture (SSM) “drydowns,” i.e., the SSM temporal dynamics following a significant rainfall event, are of particular interest when evaluating and calibrating land surface models (LSMs). By investigating drydowns, characterized by an exponential decay time scale τ, we aim to improve the representation of SSM in the ORCHIDEE global LSM. We consider τ calculated over 18 International Soil Moisture Network sites found within the footprint of FLUXNET towers, covering different vegetation types and climates. Using the ORCHIDEE LSM, we compare τ from the modeled SSM time series to values computed from in situ SSM measurements. We then assess the potential of using τ observations to constrain some water, carbon, and energy parameters of ORCHIDEE, selected using a sensitivity analysis, through a standard Bayesian optimization procedure. The impact of the SSM optimization is evaluated using FLUXNET evapotranspiration and gross primary production (GPP) data. We find that the relative drydowns of SSM can be well calibrated using observation-based τ estimates, when there is no need to match the absolute observed and modeled SSM values. When evaluated using independent data, τ-calibration parameters were able to improve drydowns for 73% of the sites. Furthermore, the fit of the model to independent fluxes was only minutely changed. We conclude by considering the potential of global satellite products to scale up the experiment to a global-scale optimization.
Activation of the MAPK pathway mediates resistance to PI3K inhibitors in chronic lymphocytic leukemia
Authors: Murali Ishwarya , Kasar Siddha , Naeem Aishath , Tyekucheva Svitlana , Khalsa Jasneet K. , Thrash Emily M. , Itchaki Gilad , Livitz Dimitri , Leshchiner Ignaty , Dong Shuai , Fernandes Stacey M. , Getz Gad , Johnson Amy , Brown Jennifer R. 
Published: March 2021

Abstract
Inhibitors of Bruton tyrosine kinase (BTK) and phosphatidylinositol 3-kinase δ (PI3Kδ) that target the B-cell receptor (BCR) signaling pathway have revolutionized the treatment of chronic lymphocytic leukemia (CLL). Mutations associated with resistance to BTK inhibitors have been identified, but limited data are available on mechanisms of resistance to PI3Kδ inhibitors. Here we present findings from longitudinal whole-exome sequencing of cells from patients with multiply relapsed CLL (N = 28) enrolled in trials of PI3K inhibitors. The nonresponder subgroup was characterized by baseline activating mutations in MAP2K1, BRAF, and KRAS genes in 60% of patients. PI3Kδ inhibition failed to inhibit ERK phosphorylation (pERK) in nonresponder CLL cells with and without mutations, whereas treatment with a MEK inhibitor rescued ERK inhibition. Overexpression of MAP2K1 mutants in vitro led to increased basal and inducible pERK and resistance to idelalisib. These data demonstrate that MAPK/ERK activation plays a key role in resistance to PI3Kδ inhibitors in CLL and provide a rationale for therapy with a combination of PI3Kδ and ERK inhibitors.
Characterization of DLBCL with a PMBL gene expression signature
Authors: Duns Gerben , Viganò Elena , Ennishi Daisuke , Sarkozy Clementine , Hung Stacy S. , Chavez Elizabeth , Takata Katsuyoshi , Rushton Christopher , Jiang Aixiang , Ben-Neriah Susana , Woolcock Bruce W. , Slack Graham W. , Hsi Eric D. , Craig Jeffrey W. , Hilton Laura K. , Shah Sohrab P. , Farinha Pedro , Mottok Anja , Gascoyne Randy D. , Morin Ryan D. , Savage Kerry J. , Scott David W. , Steidl Christian 
Published: March 2021

Abstract
Primary mediastinal large B-cell lymphoma (PMBL) is a type of aggressive B-cell lymphoma that typically affects young adults, characterized by presence of a bulky anterior mediastinal mass. Lymphomas with gene expression features of PMBL have been described in nonmediastinal sites, raising questions about how these tumors should be classified. Here, we investigated whether these nonmediastinal lymphomas are indeed PMBLs or instead represent a distinct group within diffuse large B-cell lymphoma (DLBCL). From a cohort of 325 de novo DLBCL cases, we identified tumors from patients without evidence of anterior mediastinal involvement that expressed a PMBL expression signature (nm-PMBLsig+; n = 16; 5%). A majority of these tumors expressed MAL and CD23, proteins typically observed in bona fide PMBL (bf-PMBL). Evaluation of clinical features of nm-PMBLsig+ cases revealed close associations with DLBCL, and a majority displayed a germinal center B cell–like cell of origin (GCB). In contrast to patients with bf-PMBL, patients with nm-PMBLsig+ presented at an older age and did not show pleural disease, and bone/bone marrow involvement was observed in 3 cases. However, although clinically distinct from bf-PMBL, nm-PMBLsig+ tumors resembled bf-PMBL at the molecular level, with upregulation of immune response, JAK-STAT, and NF-κB signatures. Mutational analysis revealed frequent somatic gene mutations in SOCS1, IL4R, ITPKB, and STAT6, as well as CD83 and BIRC3, with the latter genes significantly more frequently affected than in GCB DLBCL or bf-PMBL. Our data establish nm-PMBLsig+ lymphomas as a group within DLBCL with distinct phenotypic and genetic features. These findings may have implications for gene expression– and mutation-based subtyping of aggressive B-cell lymphomas and related targeted therapies.
Osteocytes regulate neutrophil development through IL-19: a potent cytokine for neutropenia treatment
Authors: Xiao Min , Zhang Wuju , Liu Wen , Mao Linlin , Yang Jincheng , Hu Le , Zhang Sheng , Zheng Yaling , Liu Anling , Song Qiancheng , Li Yuhua , Xiao Guozhi , Zou Zhipeng , Bai Xiaochun 
Published: March 2021

AbstractOsteocytes are the most abundant (90% to 95%) cells in bone and have emerged as an important regulator of hematopoiesis, but their role in neutrophil development and the underlying mechanisms remain unclear. Interleukin 19 (IL-19) produced predominantly by osteocytes stimulated granulopoiesis and neutrophil formation, which stimulated IL-19 receptor (IL-20Rβ)/Stat3 signaling in neutrophil progenitors to promote their expansion and neutrophil formation. Mice with constitutive activation of mechanistic target of rapamycin complex (mTORC1) signaling in osteocytes (Dmp1-Cre) exhibited a dramatic increase in IL-19 production and promyelocyte/myelocytic expansion, whereas mTORC1 inactivation in osteocytes reduced IL-19 production and neutrophil numbers in mice. We showed that IL-19 administration stimulated neutrophil development, whereas neutralizing endogenous IL-19 or depletion of its receptor inhibited the process. Importantly, low-dose IL-19 reversed chemotherapy, irradiation, or chloramphenicol-induced neutropenia in mice more efficiently than granulocyte colony-stimulating factor. This evidence indicated that IL-19 was an essential regulator of neutrophil development and a potent cytokine for neutropenia treatment.
Outcomes of a clinician-directed protocol for discontinuation of complement inhibition therapy in atypical hemolytic uremic syndrome
Authors: Chaturvedi Shruti , Dhaliwal Noor , Hussain Sarah , Dane Kathryn , Upreti Harshvardhan , Braunstein Evan M. , Yuan Xuan , Sperati C. John , Moliterno Alison R. , Brodsky Robert A. 
Published: March 2021

Abstract
Terminal complement inhibition is the standard of care for atypical hemolytic uremic syndrome (aHUS). The optimal duration of complement inhibition is unknown, although indefinite therapy is common. Here, we present the outcomes of a physician-directed eculizumab discontinuation and monitoring protocol in a prospective cohort of 31 patients that started eculizumab for acute aHUS (and without a history of renal transplant). Twenty-five (80.6%) discontinued eculizumab therapy after a median duration on therapy of 2.37 (interquartile range: 1.06, 9.70) months. Eighteen patients discontinued per protocol and 7 because of nonadherence. Of these, 5 (20%) relapsed; however, relapse rate was higher in the case of nonadherence (42.8%) vs clinician-directed discontinuation and monitoring (11.1%). Four of 5 patients who relapsed were successfully retreated without a decline in renal function. One patient died because of recurrent aHUS and hypertensive emergency in the setting of nonadherence. Nonadherence to therapy (odds ratio, 8.25; 95% confidence interval, 1.02-66.19; P = .047) was associated with relapse, whereas the presence of complement gene variants (odds ratio, 1.39; 95% confidence interval, 0.39-4.87; P = .598) was not significantly associated with relapse. Relapse occurred in 40% (2 of 5) with a CFH or MCP variant, 33.3% (2 of 6) with other complement variants, and 0% (0 of 6) with no variants (P = .217). There was no decline in mean glomerular filtration rate from the date of stopping eculizumab until end of follow-up. In summary, eculizumab discontinuation with close monitoring is safe in most patients, with low rates of aHUS relapse and effective salvage with eculizumab retreatment in the event of recurrence.
Tandem high-dose influenza vaccination is associated with more durable serologic immunity in patients with plasma cell dyscrasias
Authors: Branagan Andrew R. , Duffy Eamon , Gan Geliang , Li Fangyong , Foster Connor , Verma Rakesh , Zhang Lin , Parker Terri L. , Seropian Stuart , Cooper Dennis L. , Brandt Debra , Kortmansky Jeremy , Witt Davit , Ferencz Thomas M. , Dhodapkar Kavita M. , Dhodapkar Madhav V. 
Published: March 2021

Abstract
Patients with plasma cell dyscrasias (PCDs) experience an increased burden of influenza, and current practice of single-dose annual influenza vaccination yields suboptimal protective immunity in these patients. Strategies to improve immunity to influenza in these patients are clearly needed. We performed a randomized, double-blind, placebo-controlled clinical trial comparing tandem Fluzone High-Dose influenza vaccination with standard-of-care influenza vaccination. Standard-of-care vaccination was single-dose age-based vaccination (standard dose, &lt;65 years; high dose, ≥65 years), and patients in this arm received a saline placebo injection at 30 days. A total of 122 PCD patients were enrolled; 47 received single-dose standard-of-care vaccination, and 75 received 2 doses of Fluzone High-Dose vaccine. Rates of hemagglutinin inhibition (HAI) titer seroprotection against all 3 strains (H1N1, H3N2, and influenza B) were significantly higher for patients after tandem high-dose vaccination vs control (87.3% vs 63.2%; P = .003) and led to higher seroprotection at the end of flu season (60.0% vs 31.6%; P = .04). These data demonstrate that tandem high-dose influenza vaccination separated by 30 days leads to higher serologic HAI titer responses and more durable influenza-specific immunity in PCD patients. Similar vaccine strategies may also be essential to achieve protective immunity against other emerging pathogens such as novel coronavirus in these patients. This trial was registered at www.clinicaltrials.gov as #NCT02566265.
Intracellular pH regulation: characterization and functional investigation of H+ transporters in Stylophora pistillata
Authors: Capasso Laura , Ganot Philippe , Planas-Bielsa Víctor , Tambutté Sylvie , Zoccola Didier 
Published: March 2021

Abstract
Background
Reef-building corals regularly experience changes in intra- and extracellular H+ concentrations ([H+]) due to physiological and environmental processes. Stringent control of [H+] is required to maintain the homeostatic acid-base balance in coral cells and is achieved through the regulation of intracellular pH (pHi). This task is especially challenging for reef-building corals that share an endosymbiotic relationship with photosynthetic dinoflagellates (family Symbiodinaceae), which significantly affect the pHi of coral cells. Despite their importance, the pH regulatory proteins involved in the homeostatic acid-base balance have been scarcely investigated in corals. Here, we report in the coral Stylophora pistillata a full characterization of the genomic structure, domain topology and phylogeny of three major H+ transporter families that are known to play a role in the intracellular pH regulation of animal cells; we investigated their tissue-specific expression patterns and assessed the effect of seawater acidification on their expression levels.

Results
We identified members of the Na+/H+ exchanger (SLC9), vacuolar-type electrogenic H+-ATP hydrolase (V-ATPase) and voltage-gated proton channel (HvCN) families in the genome and transcriptome of S. pistillata. In addition, we identified a novel member of the HvCN gene family in the cnidarian subclass Hexacorallia that has not been previously described in any species. We also identified key residues that contribute to H+ transporter substrate specificity, protein function and regulation. Last, we demonstrated that some of these proteins have different tissue expression patterns, and most are unaffected by exposure to seawater acidification.

Conclusions
In this study, we provide the first characterization of H+ transporters that might contribute to the homeostatic acid-base balance in coral cells. This work will enrich the knowledge of the basic aspects of coral biology and has important implications for our understanding of how corals regulate their intracellular environment.