bluuebunny/crossref_metadata_2025_split huggingface dataset:
Bioactive Evaluation of Naringenin in Ameliorating Hyperuricemia‐Induced Liver Injury by Inhibiting Xanthine Oxidase
Authors: Zhang Xiaoyue , Liu Linfeng , Fan Zhaosen , Mamadalieva Nilufar , Liu Chao , Guo Xu , Sun Shutao , Sun Hui , Li Ningyang , Wang Muxuan 
Published: January 2025

ABSTRACTHyperuricemia (HUA) is one of main risk factors for liver injury, and xanthine oxidase (XOD) is an important target for HUA‐induced liver injury. As a typical natural active ingredient, naringenin (NAR) has been confirmed the good therapeutic effect on variety of diseases. However, studies of NAR ameliorating HUA‐induced liver injury have not been reported. Therefore, we evaluated the bioactivity of NAR in ameliorating HUA‐induced liver injury and investigated the related molecular mechanisms. The inhibitory activity and type of NAR on XOD was investigated by enzymatic reactions and kinetic analyses, and molecular docking showed that NAR was able to bind tightly to XOD. In vivo activity studies showed that NAR ameliorated liver function while being able to inhibit XOD activity. NAR alleviated oxidative stress in the liver caused by excess reactive oxygen species through antioxidant activity. At the same time, NAR exerted anti‐inflammatory activity by regulating the levels of inflammatory factors. The molecular docking results suggested that NAR was able to interact with Keap1 and AMPK to exhibit antioxidant and anti‐inflammatory effects. This work demonstrated the therapeutic effect of NAR on HUA‐induced liver injury, which was valuable for the further development of related functional foods.
The Effect of a Novel Functional Solid Drink on Obesity Management and Its Underlying Mechanisms
Authors: Wen Ying , Peng Yan‐Mei , Zhou Xuan‐Yu , Han Yu‐Xin , Jiang Hong‐Li , Wu Hui‐Xuan , Bu Yan‐Hong , Cheng Fei , Li Long , Xiao Fen , Cai Jun‐Min , Mo Yu‐Yao , Liang Han‐Dan , Zhou Hou‐De 
Published: January 2025

ABSTRACTObesity has become a global public health concern, yet an effective, low‐toxic, and high‐compliance strategy for its control remains elusive. This study aims to develop a novel comprehensive intervention method for the management of obesity and its complications. Thus, we designed a functional solid beverage named RLMCR, which consists of medicinal and edible Chinese herbs. We identified its chemical composition and evaluated its efficacy. Our results indicated that RLMCR was abundant in various functional ingredients, including flavonoids, polyphenols, and terpenoids. It could mitigate lipid accumulation in 3T3‐L1 cells by upregulating the expression of lipolysis‐related genes. We then established diet‐induced obese (DIO) mice models, which were administered with low, middle, and high doses of RLMCR for 8 weeks. Our findings indicated that the optimal dosage of RLMCR for weight loss was 3.0 g/kg, which effectively mitigated weight gain, lipid accumulation, hepatic steatosis, and abnormal glucose metabolism. Following treatment, oxygen consumption, carbon dioxide production, and thermogenesis were increased in DIO mice, without affecting food intake. The content of brown adipose tissue was significantly elevated, and the expression of thermogenesis‐related genes and lipolysis‐related genes in the epididymal white adipose tissue was upregulated. So, we developed a novel functional food that effectively mitigates obesity and its related metabolic disorders.
Isoelectric Focusing Fractionation Method for Signal Enhancement in Detection of Inactivated Biological Agents Using Matrix‐Assisted Laser Desorption/Ionization Mass Spectrometry
Authors: Duša Filip , Šalplachta Jiří , Horká Marie , Lunerová Kamila , Čermáková Veronika , Dřevínek Michal , Kubíček Oldřich 
Published: January 2025

ABSTRACTTimely identification of highly pathogenic bacteria is crucial for efficient mitigation of the connected harmful health effects. Matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry (MALDI‐TOF MS) of intact cells enables fast identification of the microorganisms based on their mass spectrometry protein fingerprint profiles. However, the MALDI‐TOF MS examination must be preceded by a time‐demanding cultivation of the native bacteria to isolate representative cell samples to obtain indicative fingerprints. Isoelectric focusing (IEF) is capable of separating bacterial cells according to their isoelectric point while effectively removing other non‐focusing compounds from sample matrix. In this work, we present a divergent‐flow IEF chip (DF‐IEF chip) fractionation as an alternative way for sample clean‐up and concentration of bacterial cells to prepare samples usable for following MALDI‐TOF MS analysis without the need of time‐demanding cultivation. By means of DF‐IEF chip method, we processed four species of highly pathogenic bacteria (Bacillus anthracis, Brucella abortus, Burkholderia mallei, and Yersinia pestis) inactivated with H2O2 vapors or by heat treatment at 62.5°C for 24 h. The DF‐IEF chip method continually separated and concentrated the inactivated bacterial cells for subsequent detection using MALDI‐TOF MS. The content of the inactivated bacteria in the DF‐IEF chip fractions was evaluated with the MS analysis, where inactivated Y. pestis was found to be the most efficiently focusing species. Sensitivity analysis showed limits as low as 2 × 105 colony forming units per mL for inactivated B. anthracis.
Adaptation of the disease‐related apathy scale in adults with epilepsy into Turkish: A methodological study
Authors: Turan Gülcan Bahcecioglu , Özer Zülfünaz , Başak Seda 
Published: January 2025

AbstractObjectiveThis study was conducted to culturally adapt the Epilepsy‐Related Apathy Scale in Adults with Epilepsy (E‐RAS) to Turkish and to assess its psychometric properties in adult epilepsy patients.MethodsA total of 172 epilepsy patients receiving care at the Neurology clinic and outpatient clinic of Fırat University Hospital from February to July 2023 were included in this methodological investigation. The E‐RAS was translated into Turkish, and its content and construct validity were thoroughly examined. Construct validity was assessed through exploratory factor analysis (EFA) and confirmatory factor analysis (CFA). Reliability was evaluated through item analyses, internal consistency analysis, composite reliability coefficient, and mean explained variance analysis.ResultsThe factor loadings of the scale items ranged from 0.66 to 0.89. The fit index values of the scale were X2 = 467.09, df = 245 (p <0.05), X2/df = 1.9, RMSEA = 0.073, CFI = 0.97, RMR = 0.046, SRMR = 0.057, TLI = 0.97, and AIC = 557.09. The Cronbach's alpha coefficients of the sub‐dimensions of the scale ranged from 0.880 to 0.992, and the total Cronbach's alpha coefficient was 0.928. The total McDonald's omega coefficient was 0.916, and the McDonald's omega coefficients of the sub‐dimensions ranged from 0.880 to 0.947. The Turkish form of the 24‐item and 4‐sub‐dimensional scale was validated without any changes to the original scale form.SignificanceThe Turkish adaptation of E‐RAS is a valid and reliable instrument for measuring apathy in adult epilepsy patients. Its use in clinical practice is strongly recommended.Plain Language SummaryThe E‐RAS scale can be used to assess apathy in adults with epilepsy. The was determined that the adapted Turkish form had a similar structure to the original scale. It was determined that the E‐RAS scale is valid and reliable in Turkish culture. The fact that the Turkish adaptation of the scale is similar to the original structure and other adapted cultures is a factor that facilitates its use and acceptance in international comparisons. The scale can be used to assess apathy in adults with epilepsy.
Replacement therapy in pregnant women with von Willebrand disease during delivery: Factor levels and pharmacokinetics
Authors: Al Arashi Wala , Cloesmeijer Michael E. , Leebeek Frank W. G. , Duvekot Johannes J. , Kruip Marieke J. H. A. , Mathôt Ron A. A. , Cnossen Marjon H. ,   the OPTI‐CLOT/To WiN study group and SYMPHONY consortium
Published: January 2025

AbstractLimited data are available on VWF activity (VWF:Act) and factor VIII (FVIII:C) levels during delivery after VWF/FVIII concentrate administration in women with von Willebrand disease (VWD). We aimed to evaluate treatment with a specific VWF/FVIII concentrate on factor levels in women with VWD during delivery and the postpartum period. A retrospective single‐center study was conducted between January 1, 2008, and August 1, 2022. Pregnant women treated with Haemate®P during delivery were included if they had ≥2 consecutive VWF:Act and FVIII:C measurements post‐infusion. VWF:Act/FVIII:C levels were compared to predefined target levels. A population pharmacokinetic (PopPK) model was developed, estimating VWF and FVIII pharmacokinetics after Haemate®P administration. Nineteen women were included. Targeted VWF:Act/FVIII:C peak levels were achieved after the first infusion (≥1.00 IU/mL, n = 12; ≥1.50 IU/mL, n = 5), and all VWF:Act/FVIII:C trough levels remained ≥0.50 IU/mL during first 72 h of treatment. All women had pretreatment FVIII:C levels ≥1.00 IU/mL, except one woman with type 2N, which was significantly higher than FVIII:C levels during the third trimester (median increase: 0.42 IU/mL, interquartile range: [0.12–0.92]). FVIII:C trough levels increased during treatment, median 2.05 IU/mL [1.65–2.71]. Nine women (47%) experienced postpartum hemorrhage and no thrombosis occurred. A one‐compartment PopPK model adequately described VWF:Act/FVIII:C levels. Targeted VWF:Act/FVIII:C peak levels were achieved with the prescribed dosing regimens. VWF clearance was similar to that in nonpregnant individuals. Both pretreatment and FVIIIC trough levels during treatment were high with reduced FVIII clearance. Monitoring VWF:Act/FVIII:C levels is recommended for optimizing target levels and enriching the current PopPK model, improving VWF:Act/FVIII:C level predictions, and achieving more effective dosing.
Dietary supplementation with milk‐derived Bifidobacterium animalis subsp. lactis relieves colitis‐linked reproductive disorders via gut‐testis axis
Authors: Lin Jingmin , Yin Lingzi , Fan Yueyao , Yu Zhiling , Ma Xin , Fu Xiuqiong , Zhang Yong , Tang Shaojun , Chen Jiali 
Published: January 2025

AbstractRapid urbanization, medication, and modern dietary patterns are the main challenges leading to impaired fertility, with a lack of effective therapies. Emerging evidence suggests that reproductive disorders may be closely associated with intestinal damage or occasionally worsen by the side effects of medications. Therefore, the development of dietary supplements as alternatives is crucial for intestinal‐linked reproductive health. Milk and dairy products are essential in dietary nutrition with great potential functional ingredients. Bifidobacterium animalis subsp. lactis NJ241 (NJ241), a promising probiotic isolated from naturally fermented bovine milk, remains underexplored. This study aims to investigate the molecular mechanisms of NJ241 on colitis and its associated reproductive disorders. The biomarker microbes and their correlated metabolites were further explored by 16S rRNA sequencing and metabolomics. Hematological analysis and histopathological examination were applied for conjoined identification. Results indicated that NJ241 effectively restored the expression levels of Claudin‐2 and MLCK1, reducing intestinal permeability. Multi‐omics results further revealed that NJ241 may effectively improve gut barrier integrity by increasing the abundance of Akkermansia muciniphila and its metabolite trans‐ferulic acid. This effect was accompanied by a reduction in the pro‐inflammatory cytokine IL‐6 in both serum and testicular tissue, mediated through the TLR4 signaling pathway. Consequently, the restoration of microbiota homeostasis and a systemic reduction in inflammation rescued testicular spermatogenesis, which was impaired by colitis. The current findings consistently elucidated the potential molecular mechanism by which NJ241 ameliorates colitis‐linked reproductive disorders through the gut‐testis axis. Additionally, NJ241 demonstrates promise as a probiotic supplement for the development of fortified dairy products and provides strong evidence for the potential reproductive health benefits of naturally fermented bovine milk.
The Role of Implant Coronal Surface Properties on Early Adhesion of Streptococcus Oralis—An In Vitro Comparative Study
Authors: Wang Xuesong , Liddell Robert S. , Wen Hai Bo , Davies John E. , Ajami Elnaz 
Published: January 2025

ABSTRACTDental implant coronal surfaces designed with the primary goal of maintaining crestal bone levels may also promote bacterial adhesion, leading to soft tissue inflammation and peri‐implant bone loss. Achieving an optimal surface roughness that minimizes bacterial adhesion while preserving crestal bone is crucial. It is hypothesized that a specific threshold surface roughness value may exist below which, and above which, initial bacterial adhesion does not statistically change. This study evaluated 12 commercially available and 2 custom‐designed implant surfaces for their physicochemical properties and initial bacterial adhesion, as represented by Streptococcus oralis (S. oralis) the dominant initial colonizer of the successive waves of bacterial consortia that result in plaque and biofilm formation. Implants were immersed in a S. oralis suspension for 4 h, after which microbial viability was assessed. Marked differences were observed in surface roughness, chemical composition, and wettability, and S. oralis adhesion. Surfaces with Sa > 1 μm had significantly more adherent bacteria after 4 h compared to those with Sa < 1 μm, despite complexity. Adding nanotopography to dual‐acid etched surfaces further reduced bacterial adhesion compared to surfaces without these features. The role of chemical composition and wettability was less influential than roughness. In conclusion, there is a cut‐off threshold roughness around Sa = 1 μm, above which the adhesion of bacteria increases significantly to a plateau level; while below which, bacterial adhesion is equivalent to a machined surface despite the surface texture of the implant collar.
A Closer Look at the FeS Heme Bonds in Azotobacter vinelandii Bacterioferritin: QM/MM and Local Mode Analysis
Authors: Freindorf Marek , Kraka Elfi 
Published: January 2025

ABSTRACTUsing the QM/MM methodology and a local mode analysis, we investigated a character and a strength of FeS bonds of heme groups in oxidized and reduced forms of Bacterioferritin from Azotobacter vinelandii. The strength of the FeS bonds was correlated with a bond length, an energy density at a bond critical point, and a charge difference of the F and S atoms. Changing the oxidation state from ferrous to ferric generally makes the FeS bonds weaker, longer, more covalent, and more polar. We also investigated the SFeS bond bending and found that the stronger FeS bond, generally makes the SFeS bond bending stiffer, which could play a key role in the balance between ferric and ferrous oxidation states and related biological activities.
High satisfaction and low conversion rate to total hip arthroplasty after hip arthroscopy for femoroacetabular impingement syndrome and risk factors affecting survival at long‐term follow‐up
Authors: Uzun Erdal , Ferrer Josep , Lizano Xavi , Cabello Joan , Gürsu Sarper , Pons Marc Tey 
Published: January 2025

AbstractPurposeStudies evaluating the long‐term survival rate, patient satisfaction, and conversion to total hip arthroplasty (THA) are limited. The aim of this study was to evaluate satisfaction and hip survival at a minimum 10‐year follow‐up in patients following hip arthroscopy for femoroacetabular impingement syndrome (FAIS).MethodsA total of 164 patients underwent hip arthroscopy for FAIS between 2007 and 2012; of these, 76 (49 men and 27 women; mean age, 40.7 ± 11.2 years; range, 15–69 years) patients could be clinically examined or reached by phone or email and included in the study. Prospectively collected data of these patients who followed for a minimum of 10 years were retrospectively analysed. Patients who had previous trauma or surgery on the hip or were younger than 15 years were excluded. Of the 76 patients, 37 (48.7%) had mixed type, where 29 (38.2%) had cam and 10 (13.2%) had pincer type FAIS. Patient demographics, Tonnis grade for osteoarthritis and intraoperative findings were reviewed. Outcome measures of interest included reoperations (re‐arthroscopy, conversion to THA), patient satisfaction, ability to return to sports, the Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC), modified Harris Hip Score (mHHS) and some possible factors associated with conversion to THA.ResultsThe mean follow‐up was 12.4 ± 1.8 (10–16 years). Twenty‐one per cent required conversion to THA, with a mean time requiring THA of 4.8 ± 1.7 years (2–8 years). Mean age of the patients requiring THA was 47.8 ± 8.2 years. Two (2.6%) patients required re‐arthroscopy. Fifty‐eight (76.3%) patients did not undergo any revision surgery or other treatments. Forty‐seven (61.8%) patients were completely satisfied with the results, where 5 (6.6%) patients were very satisfied and 4 (5.3%) patients were satisfied. Overall satisfaction rate was 73.7%. Mean postoperative mHHS score was 76.5 ± 15.5 and the mean WOMAC score was 84.0 ± 16.0. Return‐to‐sports rate was 85%, and the return to the old sport level was 67.1%. Age over 40 years (p = 0.004), a Tonnis Grade >1 (p < 0.001) and a full‐thickness acetabular chondral lesion (p = 0.001) were identified as factors for failure and conversion to THA.ConclusionsHip arthroscopy for FAIS resulted in high satisfaction of patients who did not eventually require THA. Higher rates of conversion to THA were seen in older patients, patients treated with higher Tonnis grade and full‐thickness acetabular chondral lesions.Level of EvidenceIV, Case Series.
Sport‐specific differences in ACL injury, treatment and return to sports: Basketball
Authors: Kocaoglu Baris , Yozgatli Tahir Koray , Tischer Thomas , Calleja‐Gonzalez Julio , Cuzzolin Francesco , Bedi Asheesh , Seil Romain 
Published: January 2025

AbstractBasketball is an intense, fast‐paced game that is physically, highly demanding. Certain aspects of the game, such as the quick pivoting and cutting movements, predispose the players to serious knee injuries, including anterior cruciate ligament (ACL) tears. While an ACL tear can be a devastating condition for players, multidisciplinary management of the injury can provide the players with a reasonable chance to return to play at the pre‐injury level. This article aims to review the general principles and guidelines for the management of ACL injury in basketball players. The diagnosis, surgical treatment, rehabilitation and return to sports are discussed from European and North American perspectives. With a comprehensive and multidisciplinary approach to this condition, medical professionals can provide injured basketball players with a favourable prognosis for returning to play at the pre‐injury level. To return to normal life and basketball after ACL reconstruction, the proposed approach includes (1) the selection of the appropriate surgical graft and technique, (2) maintaining a healthy and persistent mental state during rehabilitation, and (3) following a scientific rehabilitation programme based on personalized recovery.Level of EvidenceLevel V.
Vegetation Resilience Evaluation of Coal Waste Dumps After Reclamation in Arid and Semi‐Arid Mining Areas Based on Temporal Satellite Imagery
Authors: Zhang Lifan , Zhao Yanling , Ren He , Xiao Wu , Li Zhibin 
Published: January 2025

ABSTRACTOpen‐pit mine waste dumps often exhibit unstable ecological recovery due to the limitations of artificial reconstruction process, despite undergoing land reclamation efforts. Especially in arid and semi‐arid mining regions, where harsh climatic conditions and drought events often occur, exacerbating the vulnerability of vegetation to degradation and hinder restoration efforts. Therefore, it is crucial to conduct continuous monitoring to ensure the stability of vegetation restoration and succession of coal waste dumps after reclamation. This study employed Landsat time series imagery combined with BFAST change detection algorithm to evaluate the vegetation resilience of two reclaimed coal waste dumps, the North coal waste dump (NCWD) and the South coal waste dump (SCWD), in a typical arid and semi‐arid mining area in China, focusing on both resistance and recovery rate. The results viewed that all pixels across both dumps experienced 2–5 breakpoints from 2013 to 2021. Compared to the NCWD, the SCWD exhibited fewer breakpoints overall. 86.11% and 93.31% of pixels in NCWD and SCWD, respectively, can be effectively recovered after a long‐term drought. However, the recovery rate was generally low, primarily concentrated in 0–0.002, highlighting the negative impact of extreme drought events on vegetation restoration. Vegetation resilience exhibited a distinct spatial distribution influenced by terrain characteristics. Generally, steeper slope, higher elevation, and windward direction (N‐W) were associated with lower vegetation resistance. Besides, vegetation status before severe drought significantly influenced the vegetation resilience. The NDVI before drought events were positively correlated to the recovery rate of NCWD (r = 0.318, p < 0.01) and SCWD (r = 0.301, p < 0.01), respectively. It was also observed the positive effect of artificial management on vegetation resilience of coal waste dumps, as shown by the superiority of SCWD to NCWD in terms of resistance and recovery rate. Our research provided valuable reference for land reclamation and post‐reclamation management measure of coal waste dumps in arid and semi‐arid mining areas.
Fluid dynamics of dinoflagellate feeding and swimming
Authors: Schuech Rudi , Nielsen Lasse Tor , Humphries Stuart , Smith Dave , Kiørboe Thomas 
Published: January 2025

AbstractFlagella are crucial to the interactions of unicellular organisms with their surrounding aquatic environment. One ecologically important group of flagellates, the dinoflagellates, has a unique flagellar arrangement consisting of a trailing and a transversal flagellum. The latter is recessed within a groove around the cell and drives a hair‐bearing membrane that undulates with a helical beat. Dinoflagellates are further unique by having clearance rates that are an order of magnitude higher than those of other similarly sized phagotrophic flagellates, overlapping in size and swimming speed with ciliates. Here, using flow visualization and computational fluid dynamics, we show how this arrangement of just two flagella propels these large cells at high speeds and allows very high clearance rates. We find that the transverse flagellum provides most of the forward thrust, whereas the trailing flagellum is mainly for steering. The flagellar hairs and the sheet‐like structure of the transverse flagellum allow dinoflagellates to exert strong propulsive forces at high efficiency without extending a long flagellum far into the surrounding fluid. The unique flagellar arrangement of dinoflagellates may therefore be key to their evolutionary success.