bluuebunny/crossref_metadata_2025_split huggingface dataset:
Maximum baryon masses for static neutron stars in f(R) gravity
Authors: Astashenok A. V. , Capozziello S. , Odintsov S. D. , Oikonomou V. K. 
Published: November 2021

Abstract
We investigate the upper mass limit predictions of the baryonic mass for static neutron stars in the context of f(R) gravity. We use the most popular f(R) gravity model, namely the R
2 gravity, and calculate the maximum baryon mass of static neutron stars adopting several realistic equations of state and one ideal equation of state, namely that of causal limit. Our motivation is based on the fact that neutron stars with baryon masses larger than the maximum mass for static neutron star configurations inevitably collapse to black holes. Thus with our analysis, we want further to enlighten the predictions for the maximum baryon masses of static neutron stars in R
2 gravity, which, in turn, further strengthens our understanding of the mysterious mass gap region. As we show, the baryon masses of most of the equations of states studied in this paper lie in the lower limits of the mass gap region

–

, but intriguingly enough, the highest value of the maximum baryon masses we found is of the order of

. This upper mass limit also appears as a maximum static neutron star gravitational mass limit in other contexts. Combining the two results which refer to baryon and gravitational masses, we point out that the gravitational mass of static neutron stars cannot be larger than three solar masses, while based on maximum baryon masses results of the present work, we can conspicuously state that it is highly likely the lower mass limits of astrophysical black holes in the range of

–

. This, in turn, implies that maximum neutron star masses in the context of R
2 gravity are likely to be in the lower limits of the range of

–

. Hence our work further supports the General Relativity claim that neutron stars cannot have gravitational masses larger than

and then, to explain observations comparable or over this limit, we need alternative extensions of General Relativity, other than f(R) gravity.
The spectral line asymmetry of the Doppler effect in relativistic plasmas
Authors: Gossa H. , Meftah M. T. , Chenini K. , Zenkhri D. E. , Amieur B. , Guerrida H. 
Published: November 2021

Abstract
In this work, we present a comparative study between the relativistic and non-relativistic Doppler effects on spectral line profiles in ultra-hot plasmas in a laboratory system. We have established an exact formula of the relativistic Doppler profile in ultra-high-temperature plasma that is not a Gaussian one (unlike the non-relativistic Doppler profile that is Gaussian). We have also derived a new FWHM (Full Width at Half Maximum) formula of the corresponding profile that is different from the non-relativistic FWHM (

). We have also shown that, in the relativistic case, Doppler broadening exhibits an asymmetry of spectral line profile (non-Gaussian profile). To ensure the validity of our investigation, we have compared our theoretical calculation with the experimental results and found a good agreement.
The mechanosensitive channel Piezo1 cooperates with semaphorins to control neural crest migration
Authors: Canales Coutiño Brenda , Mayor Roberto 
Published: November 2021

ABSTRACT
Cells are permanently exposed to a multitude of different kinds of signals: however, how cells respond to simultaneous extracellular signals within a complex in vivo environment is poorly understood. Here, we studied the role of the mechanosensitive ion channel Piezo1 on the migration of the neural crest, a multipotent embryonic cell population. We identify that Piezo1 is required for the migration of Xenopus cephalic neural crest. We show that loss of Piezo1 promotes focal adhesion turnover and cytoskeletal dynamics by controlling Rac1 activity, leading to increased speed of migration. Moreover, overactivation of Rac1, due to Piezo1 inhibition, counteracts cell migration inhibitory signals by Semaphorin 3A and Semaphorin 3F, generating aberrant neural crest invasion in vivo. Thus, we find that, for directional migration in vivo, neural crest cells require a tight regulation of Rac1, by semaphorins and Piezo1. We reveal here that a balance between a myriad of signals through Rac1 dictates cell migration in vivo, a mechanism that is likely to be conserved in other cell migration processes.
A 37 kb region upstream of brachyury comprising a notochord enhancer is essential for notochord and tail development
Authors: Schifferl Dennis , Scholze-Wittler Manuela , Wittler Lars , Veenvliet Jesse V. , Koch Frederic , Herrmann Bernhard G. 
Published: November 2021

ABSTRACT
The node-streak border region comprising notochord progenitor cells (NPCs) at the posterior node and neuro-mesodermal progenitor cells (NMPs) in the adjacent epiblast is the prime organizing center for axial elongation in mouse embryos. The T-box transcription factor brachyury (T) is essential for both formation of the notochord and maintenance of NMPs, and thus is a key regulator of trunk and tail development. The T promoter controlling T expression in NMPs and nascent mesoderm has been characterized in detail; however, control elements for T expression in the notochord have not been identified yet. We have generated a series of deletion alleles by CRISPR/Cas9 genome editing in mESCs, and analyzed their effects in mutant mouse embryos. We identified a 37 kb region upstream of T that is essential for notochord function and tailbud outgrowth. Within that region, we discovered a T-binding enhancer required for notochord cell specification and differentiation. Our data reveal a complex regulatory landscape controlling cell type-specific expression and function of T in NMP/nascent mesoderm and node/notochord, allowing proper trunk and tail development.
SMAD4 target genes are part of a transcriptional network that integrates the response to BMP and SHH signaling during early limb bud patterning
Authors: Gamart Julie , Barozzi Iros , Laurent Frédéric , Reinhardt Robert , Martins Laurène Ramos , Oberholzer Thomas , Visel Axel , Zeller Rolf , Zuniga Aimée 
Published: November 2021

ABSTRACT
SMAD4 regulates gene expression in response to BMP and TGFβ signal transduction, and is required for diverse morphogenetic processes, but its target genes have remained largely elusive. Here, we identify the SMAD4 target genes in mouse limb buds using an epitope-tagged Smad4 allele for ChIP-seq analysis in combination with transcription profiling. This analysis shows that SMAD4 predominantly mediates BMP signal transduction during early limb bud development. Unexpectedly, the expression of cholesterol biosynthesis enzymes is precociously downregulated and intracellular cholesterol levels are reduced in Smad4-deficient limb bud mesenchymal progenitors. Most importantly, our analysis reveals a predominant function of SMAD4 in upregulating target genes in the anterior limb bud mesenchyme. Analysis of differentially expressed genes shared between Smad4- and Shh-deficient limb buds corroborates this function of SMAD4 and also reveals the repressive effect of SMAD4 on posterior genes that are upregulated in response to SHH signaling. This analysis uncovers opposing trans-regulatory inputs from SHH- and SMAD4-mediated BMP signal transduction on anterior and posterior gene expression during the digit patterning and outgrowth in early limb buds.
The endoplasmic reticulum–plasma membrane tethering protein TMEM24 is a regulator of cellular Ca2+ homeostasis
Authors: Xie Beichen , Panagiotou Styliani , Cen Jing , Gilon Patrick , Bergsten Peter , Idevall-Hagren Olof 
Published: November 2021

ABSTRACT
Endoplasmic reticulum (ER)–plasma membrane (PM) contacts are sites of lipid exchange and Ca2+ transport, and both lipid transport proteins and Ca2+ channels specifically accumulate at these locations. In pancreatic β-cells, both lipid and Ca2+ signaling are essential for insulin secretion. The recently characterized lipid transfer protein TMEM24 (also known as C2CD2L) dynamically localizes to ER–PM contact sites and provides phosphatidylinositol, a precursor of phosphatidylinositol-4-phosphate [PI(4)P] and phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2], to the PM. β-cells lacking TMEM24 exhibit markedly suppressed glucose-induced Ca2+ oscillations and insulin secretion, but the underlying mechanism is not known. We now show that TMEM24 only weakly interacts with the PM, and dissociates in response to both diacylglycerol and nanomolar elevations of cytosolic Ca2+. Loss of TMEM24 results in hyper-accumulation of Ca2+ in the ER and in excess Ca2+ entry into mitochondria, with resulting impairment in glucose-stimulated ATP production.
Superoxide dismutase and the sigma1 receptor as key elements of the antioxidant system in human gastrointestinal tract cancers
Authors: Skrzycki Michał 
Published: November 2021

Abstract
This long-term research was designed to evaluate whether superoxide dismutase (SOD) isoenzymes participate in the development of human gastrointestinal neoplasms and the potential influence of the sigma1 receptor (Sig1R) on the regulation of SOD gene expression during the neoplastic process. The experiments included human tissues from selected gastrointestinal tract tumors (liver cancer, colorectal adenocarcinoma, and colorectal cancer liver metastases). Activity, protein levels, and mRNA levels were determined for SOD isoenzymes and Sig1R. Additionally, markers of oxidative stress (glutathione, lipid peroxidation) were measured. The results showed significant changes in the antioxidant system activity in all examined types of tumors. SOD changed both in healthy cells and in neoplastic cells. The activity and expression of all studied enzymes significantly changed due to the advancement of tumor development. The Sig1R might be an additional regulator of the antioxidant system on which activity might depend on the survival and proliferation of cancer cells. Overall, the study shows that SOD1 and SOD2 are involved not only in the formation of neoplastic changes in the human gastrointestinal tissues (healthy intestine – colon tumor; healthy liver – liver cirrhosis – liver cancer) but also in the development of tumors in the sequence: benign tumor – malignant tumor – metastasis.
Oxy+ (arthrospira) and its medicinal importance: an appraisal
Authors: Alam Md Anzar , Quamri Mohd Aleemuddin , Ahmad Bhat Muzafar Din , Aafreen Siddiqui , Sofi Ghulamuddin 
Published: November 2021

Abstract
Oxy+ is a natural source of arthrospira found in nature, used as a dietary supplement and manufactured in Aruba for lifefactors. Arthrospira contains good quality of proteins, sulfated polysaccharides, γ-linoleic acid, along with an array of carotene and phytopigments, vitamins, and minerals which are reported to be antioxidant, immunomodulator, antihyperglycemic, antidyslipidemic, cardioprotective, hepatoprotective, antiviral, anticancerous, antihypertensive, anti-inflammatory, analgesic, neuroprotective and renoprotective activities. Several studies have shown arthrospira, and active ingredients of it revealed various pharmacological activities. It can be used for the management of various ailments such as diabetes, dyslipidemia, obesity, hypertension, cancer, arthritis, osteoarthritis, autoimmune disorders, etc. This review attempts to explore the hidden benefits of Oxy+ (arthrospira).
Ocimum gratissimum leaf extract ameliorates phenylhydrazine-induced anaemia and toxicity in Wistar rats
Authors: Akara Emmanuel U. , Emmanuel Okezie , Ude Victor C. , Uche-Ikonne Chikezie , Eke Godswill , Ugbogu Eziuche Amadike 
Published: November 2021

Abstract

Objectives

Ocimum gratissimum L. is used in traditional medicine for the treatment of bacterial infections and anaemia. This study was designed to evaluate the effect of O. gratissimum leaf extract on phenylhydrazine (PHZ)-induced anaemia and toxicity in rats.

Methods
The experimental rats were divided into five groups (A–E) (n=6/sex/group). Each rat in groups B–E was intraperitoneally administered 50 mg/kg of PHZ for two consecutive days. Group A (normal control) did not receive any PHZ, group B (negative control), group C received orally 5 mg/kg ferrous sulphate whereas groups D and E received 200 and 400 mg/kg O. gratissimum leaf extract respectively, for 14 days.

Results
Red blood cell count, packed cell volume, haemoglobin concentration and high-density lipoprotein increased significantly (p<0.05) whereas low-density lipoprotein and very-low-density lipoprotein decreased in extract-treated groups when compared to the negative control. O. gratissimum (400 mg/kg extract) and standard drug (5 mg/kg ferrous sulphate) significantly (p<0.05) reduced the levels of aspartate aminotransferase, alanine aminotransferase, and alkaline phosphatase.

Conclusions
The results of this study indicate that O. gratissimum leaf extract has a restorative effect on the phenylhydrazine-induced metabolic distortions in the blood, liver, and kidney, and therefore could be used therapeutically as an anti-anaemic tonic.
Influence of paper properties on adhesive strength of starch gluing
Authors: Dohr Claudia Anna , Hirn Ulrich 
Published: November 2021

Abstract
The effect of paper properties on the strength of starch gluing for Kraft sack papers has been investigated. We analyzed the effect of surface roughness, wettability and glue penetration. Surface roughness was found not to be related to gluing strength, also surface wetting measured by contact angle showed only a weak relation. Liquid penetration measured by ultrasound (ULP) was found to have a substantial correlation to gluing strength. Comparing ULP liquid penetration speed with actual glue uptake during the gluing process we found that they are only moderately connected. We are attributing this to the fact that the penetration and spreading of the glue on the paper is driven by applying an external pressure during the gluing process, which is not the case for the liquid penetration measurement. Investigating how asymmetrical glue penetration affects gluing strength we found that the relationship was low. The best indication for gluing strength turned out to be the surface wetting/substrate swelling parameter from the ultrasonic liquid penetration measurement. We conclude that the main parameter capturing gluing strength combines the influence of fiber wetting and penetration of the glue into the fibers.
Study of gold nanoparticles’ preparation through ultrasonic spray pyrolysis and lyophilisation for possible use as markers in LFIA tests
Authors: Jelen Žiga , Majerič Peter , Zadravec Matej , Anžel Ivan , Rakuša Martin , Rudolf Rebeka 
Published: November 2021

Abstract
To monitor the progress and prevent the spread of the COVID-19 pandemic in real time and outside laboratories, it is essential to develop effective tests that can ensure rapid, selective, and reliable diagnosis of infected persons in different environments. Key in this regard is the lateral flow immunoassays (LFIAs) that can detect the presence of the SARS-CoV-2 virus quickly, with the aid of nanoparticles (NPs) and specific proteins. We report the use of gold (Au) NPs AuNPs synthesised from a gold(iii) chloride tetrahydrate precursor in a USP device and collected in a suspension composed of deionised water with polyvinylpyrrolidone as a stabiliser and cryoprotectant. In combination with freeze-drying of the AuNPs’ suspension to achieve water elimination, improved stability, and the target concentration, they exhibit the necessary properties for use as markers in LFIA rapid diagnostic tests. This was confirmed by complementary characterisation determined by using the techniques including inductively coupled plasma-optical emission spectrometry, dynamic light scattering method and zeta-potential, ultraviolet-visible spectroscopy, X-ray diffraction, scanning electron microscopy with energy dispersion spectrometer, and transmission electron microscopy, as well as with the preparation of a prototype LFIA test strip with AuNPs. Thus, such AuNPs, as well as the USP method, show promise for the development of new markers for use in LFIA.
COVID-19 and Second Home Prices in Switzerland: An Empirical Insight
Authors: Bieger Thomas , Weinert Robert , Klumbies Aristid 
Published: November 2021

Abstract
During the COVID-19 pandemic, second home ownership created several owner benefits. This paper analyses price changes of second homes during the pandemic. It derives propositions for the impact of the pandemic on the value of second homes for its owners. The contribution draws on panel data of transaction prices for second homes from Switzerland, a country with traditionally strong second home ownership, provided by Wüest Partner. The results show that there is a significant price increase for second homes – especially compared to apartments – after the start of the COVID-19 crisis. They also show that prices even in certain second-class destinations have risen significantly during the pandemic. Different research propositions are derived like that buyers might look for less crowded places in the pandemic, and the reduced benefits of intensive infrastructures during a pandemic.
Estratégias discursivas da Vale S.A. e a construção do pensamento sobre o desenvolvimento da Amazônia
Authors: Santos Larissa Pereira , Fonseca de Castro Fábio , Amorim Célia Regina Trindade Chagas 
Published: November 2021

A mineradora Vale S.A. é uma empresa transnacional, presente em 30 países, sendo uma das maiores mineradoras do mundo. Na Amazônia brasileira ela atua há mais de três décadas, principalmente com o Programa Grande Carajás (PGC) e recentemente com o Projeto S11D, projeto de ampliação das atividades de extração de minério de ferro, na Serra de Carajás, no estado do Pará. Por meio da Análise do Discurso e da Enunciação (VERÓN, 2004), essa pesquisa busca analisar como são construídos os discursos produzidos no site da mineradora, com a página intitulada “Complexo S11D Eliezer Batista”, a partir da ideia de desenvolvimento divulgada pela empresa. O S11D é considerado pelos críticos do setor da mineração, como a continuidade do PGC, agora com perspectivas de lucros duplicados. A análise considerou que a mineradora contribui para a construção de um pensamento sobre desenvolvimento na Amazônia, assentado na ideia de crescimento econômico, ignorando os discursos que representam os territórios e sujeitos sociais da região onde atua.
Comparison of patients’ satisfaction with eye care and family medicine services at a primary health centre in Ogun State, Nigeria
Authors: Betiku Anthony O. , Folashade Akinsola B. , Aribaba Olufisayo T. , Jagun Omodele O. , Oduyoye Omobola O. 
Published: November 2021

Background: Patient satisfaction need to be assessed to enable the health care provider assess the quality of care from the patients’ perspective. This study aimed to assess and compare the level of patient satisfaction with the two main health services (primary eye care and family medicine services) at primary health centre (PHC), Pakoto, Ifo local government area of Ogun State, Nigeria.Methods: This study was a descriptive cross-sectional study. A total of 280 adult patients attending the eye clinic and family medicine clinic at the PHC, Pakoto were recruited. A structured, interviewer-administered questionnaire-the patient satisfaction questionnaire (PSQ-III) was used. Data analysis was done using Epi Info 7.0.9.7 version.Results: The average overall mean score was higher in the eye clinic (3.7±0.4) than the family medicine clinic (3.5±0.3) and mean difference was statistically significant (p=0.004, t=-2.876). Most listed areas of dissatisfaction by respondents in the eye clinic were waiting period (17.2%) and number and visiting days of doctors (19%). However, at the family medicine clinic the most listed areas of dissatisfaction were all aspects of medical care (17.7%), electricity (17.7%) and toilet facilities (14.7%). There was significant association between income and general satisfaction (p=0.001)Conclusions: Most patients were generally satisfied with medical care at both clinics studied at the PHC, Pakoto. However, areas of dissatisfaction included waiting period, number of doctors and visiting days and provision of basic amenities. The study recommends that doctors should be encouraged to reside at the PHC, Pakoto and there should be provision of good basic amenities.
Demographic variables and clinical profile of scrub typhus patients: experience from a tertiary care center in Udaipur, Rajasthan
Authors: Chaudhary Suresh , Dixit Mukul , Verma Anjana , Mittal Hemlata , Mathur Medha 
Published: November 2021

Background: Scrub typhus is an acute febrile bacterial zoonoses, caused by Orientia tsutsugamushi and is associated with a range of complications, including sepsis, Acute respiratory distress syndrome (ARDS), pre-renal azotemia and Multiorgan dysfunction syndrome (MODS). The objective of this study was to study the demographic variables and clinical profile of the patients diagnosed with scrub typhus. admitted in a tertiary care hospital in Udaipur, Rajasthan.Methods: It was a longitudinal, observational study, conducted in a tertiary care centre in Udaipur, Rajasthan from January 2020-June 2021. All patients, diagnosed (positive for antibody IgM) with scrub typhus in the hospital during the study period were included in the study. Information about the patients was collected using a pre-tested questionnaire and clinical examination, after taking the informed consent from the study participants.Results: Mean age of the patients was 36.4±19 years. Majority (69.4%) of the patients were from rural area and fever was the most common (83.9%) symptom experienced. Eschar was found in only 1.6% of patients. About 87.1% of the patients had complications, with MODS, being the most common affecting about 34% of the total patients. The patients from rural areas were found to have significantly higher odds (OR=1.61) of having complications as compared to urban area patients (CI=1.09-4.3). Conclusions: This article draws attention to the fact that scrub typhus is quite on rise and most of the cases are associated with complications. There is a need to develop effective strategies and interventions to stop this rampant rise of the cases in India.
Lessons for pandemic management in rural healthcare settings in India: a retrospective cohort study of COVID-19 in a rural COVID care centre
Authors: R. Seetharam Mysore , M. Vidyashree , Shields Sydney , Suryanarayana Kumar Gavali , Kalliath Priyanka Catherine Mani , Naik Vishwesh Vishnu , Thambad Mohan Kumar B. , Srinivasaiyer Anantha Kumar 
Published: November 2021

Background: The COVID-19 pandemic has affected billions of lives across all countries precipitating an unprecedented demand for simultaneous curative, preventative, promotive, and curative services which has impacted the entire healthcare system. Even as it continues to rage across the globe, it is pertinent to learn from current experiences to build resilience into the system, especially for the vulnerable populations in rural areas.Methods: A retrospective cohort study of COVID-19 was conducted at COVID care centre in Vivekananda Memorial Hospital. A total of 136 COVID-19 confirmed patients were included in the study. Epidemiological and clinical profiles of patients were summarised. The association between risk factors and COVID-19 disease progression and patients’ outcomes at discharge were analysed and adjusted risk ratios were presented.Results: Out of the 136 patients, 128 were discharged uneventfully, 7 were referred to a higher centre because of disease progression and one patient died due to cardiac arrest. The study showed statistically significant association between age above 50 years and the presence of diabetes with COVID-19 disease progression. Delayed presentation for care also carried a higher risk of negative outcomes. Significant number of diabetics had derangement of their glycaemic control during the period of study.Conclusions: The study has highlighted the importance of ensuring essential non-COVID public health services, especially targeted follow-up of older adults, people with diabetes for their treatment adherence and early detection and treatment initiation of COVID-19 amongst them.