bluuebunny/crossref_metadata_2025_split huggingface dataset:
Impact of Helicobacter Pylori Eradication Therapy on Gastric Microbiome
Authors: Mao Liqi , Zhou Yanlin , Wang Shuangshuang , Chen Lin , Hu Yue , Yu Leimin , Xu Jingming , Lyu Bin 
Published: April 2021

Abstract
Background: Helicobacter pylori (Hp) eradication therapy has been used in clinical practice for many years. Yet, the effect of this therapy on existing gastric microflora has not been well understood. In this study, we explored the effect of eradication therapy on the microbial community in the stomach and the specific recovery after the successful eradication therapy. Methods: Among the 89 included patients, 23, 17, 40, and 9 were enrolled into the Hp-negative, Hp-positive, Successful eradication, and Failed eradication groups, respectively. Four subgroups were further divided according to disease status (Hp-negative chronic gastritis [N-CG], Hp-negative atrophic gastritis [N-AG], successful-eradication chronic gastritis [SE-CG], and atrophic gastritis with successful eradication [SE-AG]). During the endoscopic examination, one piece of gastric mucosa tissue was obtained from the lesser curvature side of the gastric antrum and gastric corpus, respectively. 16S rRNA gene sequencing was used to analyze the gastric mucosal microbiome. Results: In Hp negative group, the gastric microbiota was dominated by five phyla: Firmicutes , Proteobacteria , Actinobacteria , Bacteroidetes, and Fusobacteria . Two Hp-related genera were selected as potential biomarkers: Curvibacter and Acinetobacter . After successfully eradicating Hp, the bacterial flora in the stomach recovered to a considerable extent, and the failure of eradication was almost unchanged compared with Hp positive subjects. SE-CG was characterized by an increase in Firmicutes taxa and a decrease in Proteobacteria taxa compared with N-CG. SE-AG was characterized by a decrease in Firmicutes relative to N-AG. Finally, no differences were found in pairwise comparisons of nitrate and nitrite reductase functions among the four subgroups. Conclusions: After Hp infection, the diversity and relative abundance of gastric microflora were significantly decreased. Yet, gastric microbiota could be partially restored to the Hp-negative status after eradication; however, this effect was incomplete and might contribute to the long-term risks.
Supervillin contributes to LPS-induced inflammatory response in THP-1 cell-derived macrophages
Authors: Zhou Jun , Que Yuhui , Pan Lihua , Li Xu , Zhu Chao , Jin Lai , Li Shengnan 
Published: April 2021

Abstract
Supervillin (SVIL), the largest member of villin/gelsolin family, is an actin-binding and membrane-associated protein, that can also be localized to the nucleus. It has been reported that the mRNA expression of SVIL in neutrophils could be increased by lipopolysaccharide (LPS), but the underlying mechanisms remain unknown. Moreover, SVIL was also observed to be involved in the regulation of macrophages’ movement. However, it is not clear whether SVIL is involved in the LPS-induced inflammatory response in macrophages. This work was to investigate the underlying molecular mechanisms of LPS regulating SVIL expression in macrophages and hence the possible role of SVIL in LPS-induced inflammation. Our data showed that in THP-1-derived macrophages, LPS stimulation significantly increased SVIL mRNA and protein expression. Inhibition of TLR4 by Resatorvid (Res) completely reversed the expression of SVIL and inflammatory cytokines (IL-6, IL-1β and TNF-α) induced by LPS. Additionally, ERK1/2 and NF-κB inhibitors (U0126 and BAY) significantly reduced SVIL and IL-6, IL-1β & TNF-α expression. Furthermore, down-regulation of SVIL by SVIL-specific shRNA significantly attenuated the expression of IL-6, IL-1β & TNF-α induced by LPS. Taken together, as a downstream molecule of TLR4/NF-κB and ERK1/2, SVIL was involved in the inflammatory response of LPS-induced elevated IL-6, IL-1β and TNF-α in macrophages.
Prognostic Value of Serum MICA Levels as a Marker of Severity in COVID-19 Patients
Authors: Farzad Faramarz , Yaghoubi Neda , Azad Farahzad Jabbari , Mahmoudi Mahmoud , Mohammadi Mojgan 
Published: April 2021

Abstract
Purpose: The global pandemic of COVID-19 and high mortality rates, necessitate the development of diagnostic and prognostic tools and expansion of testing capacity. The existing approaches to identification and characterization of SARS-COV-II infection are usually based on viral genome detection or measuring COVID-19-specific antibody levels. Despite being valuable, these methods are unable to anticipate disease outcomes in patients. Given the point that the innate immune cells, particularly NK cells, ‌contribute significantly to anti-viral defense, this research aimed to determine the prognostic value of serum secretory MHC class I polypeptide–related sequence A (sMICA) levels as an important ligand for NKG2D receptor, the master regulator of NK cell development and responsiveness.Methods: Serum levels of MICA were measured by ELISA assay. Sera (n = 60) were collected from SARS-CoV-2 positive patients and disease severity was assessed by clinical criteria. The patient group consisted of 30 cases with mild disease and 30 severely ill patients examined versus 30 controls. Results: Our data revealed that serum levels of MICA were considerably higher in patients compared with controls, particularly in cases with severe complications (P<0.0001). Conclusion: Higher serum levels of MICA may be associated with respiratory failure in COVID-19 and can serve as a marker of clinical severity for patients with SARS-CoV-2 infection in particular whenever the clinical manifestations are not sufficient to make a confident prediction.
Manure Applications Combined With Chemical Fertilizer Improves Soil Functionality, Microbial Biomass and Rice Production in a Paddy Field
Authors: Iqbal Anas , He Liang , McBride Steven G , Ali Izhar , Akhtar Kashif , Khan Rayyan , Zaman Maid , We Shangqin , Guo Zixiong , Jiang Ligeng 
Published: April 2021

Abstract
Synthetic fertilizer with organic fertilizer (OF) is an approach for the improvement of soil health and quality without compromising crop yield. Therefore, a two-year field experiment was conducted to explore optimal chemical fertilizer (CF) management strategies in the context of OF, such as cattle manure (CM) and poultry manure (PM) fertilization to Ultisol soil to improve soil microbial biomass production, enzyme activities and nutrient contents, as well as grain yield of rice. A total of six treatments in the following combinations were used: i.e., T1— CF0; T2—100% CF; T3—60% CM + 40% CF; T4—30% CM + 70%CF; T5—60% PM + 40% CF, and T6—30% PM + 70% CF. Results showed that the combined fertilization significantly increased soil enzymatic activities such as soil invertase, acid phosphatase, urease, catalase, ꞵ-glucosidase, and cellulase as compared to sole CF application. Similarly, the integrated manure and inorganic fertilizers led to significant increases in soil microbial biomass carbon (MBC), microbial biomass nitrogen (MBN), soil pH, soil organic carbon (SOC), total nitrogen (TN), available nitrogen (AN), available phosphorous (AP) and grain yield of rice. Average increases in soil MBC, MBN, SOC AN, and AP in the 0–20 cm soil depth were 62.2%, 54.5%, 29.2%, 17.4%, and 19.8%, respectively, across the years in treatment T3 compared with T2. Interestingly, the linear regression analysis displayed that soil enzymatic activities were highly positively correlated with MBC and MBN. Furthermore, the PCA exhibited that the improved soil enzyme activities and microbial biomass production played a key role in the higher grain yield of rice. Overall, the results of this study demonstrate that the combined use of CF and OF in paddy soil could be beneficial for the farmers in southern China by improving soil functionality and yield of rice on a sustainable basis.
Is There a Clinical Significance of Very Low Serum Immunoglobulin E Level?
Authors: Al Serdar , Asilsoy Suna , Uzuner Nevin , Atakul Gizem , Atay Özge , Kangallı Özge , Al Işık Odaman , Karaman Özkan 
Published: April 2021

Abstract
Purpose: It has been well known that high serum immunoglobulin (Ig) E levels are associated with allergies, parasitic infections and some immune deficiencies; however, the potential effects and clinical implications of low IgE level on the human immune system are not well known. To determine the disorders accompanying very low IgE levels in children and adults. Methods: The patients whose IgE levels were determined between January 2015 and September 2020 were analyzed, and the ones with an IgE level <2.5 IU/mL were included in the study. Demographic data, immunoglobulin levels, auto-antibody results, and the diagnoses of the patients were noted from the electronic recording system of the hospital. Result: The IgE levels were measured in 34,809 patients (21,875 children, 12,934 adults), and 180 patients had IgE levels <2.5 IU/mL. Eighty patients were children (0.37%), 100 were adults (0.77%). There was a malignant disease in 45 (11 of them children) (25%), autoimmune diseases in 30 (4 of them children) (16.7%) and immunodeficiency in 19 (16 of them children) (10.6%) of the patients. The most common reasons were other disases, immunodeficiency and malignancy in children; and malignancy, autoimmune disorders and other diseases in the adults, in rank order. The IgE level did not show any correlation with the levels of other immunoglobulins.Conclusion: Although rare, a low IgE level has been shown to accompany malignancies, autoimmune disorders and immune deficiencies. Patients with very low IgE levels should be carefully monitored for systemic disorders.
Turnover and Retention Strategies Among Mental Health Workers Debbie L. Youngdoctor of Business Administrationtrophy Club, Texas Debyoungwahu@yahoo.com, (254) 421-4134orcid Id Https://orcid.org/0000-0001-6073-8160
Authors: Young Debbie L 
Published: April 2021

Abstract
PurposeThe purpose of this study was to explore retention strategies that healthcare administrators use to retain mental health workers in community mental health clinics. The study was guided by the question What strategies do HCAs use to retain their MHWs with more than 2 years of experience? MethodsA qualitative case study analysis was performed. Interviews were conducted with six healthcare administrators at Fort Worth area community mental health clinics. Interviews were coded using thematic analysis. To increase the study’s reliability and validity, the interview guide was field checked by experts and member checking performed on interview transcripts.ResultsThe healthcare administrators identified six strategies for retention: providing good benefits packages, promoting worker health and wellness, providing competitive salaries, giving workers flexible scheduling options, incentivizing workers with rewards and positive reinforcement, and maintaining open communication channels.ConclusionsThe results from this study have positive implications for mental health workers, patients, and community mental health clinics. The strategies identified in this study can be implemented to increase worker satisfaction and reduce turnover. In doing so, patients will receive higher quality care and organizations will be more attractive to prospective employees.
Quality of Life in Keratoconus: Evaluation With Keratoconus Outcomes Specific Questionnaire (KORQ)
Authors: pinto roberto damian pacheco , Abe Ricardo Yuji , Gomes Flavia Cid , Barbisan Paulo Rodolfo Tagliari , Martini Alexandre Fattah , Borges Daniel de Almeida , Fernandes Arthur gustavo , Arieta Carlos Eduardo Leite , Alves Monica 
Published: April 2021

Abstract
Purpose: To assess quality of life in keratoconus patients using the Keratoconus Outcomes Research Questionnaire (KORQ) translated and validated to Portuguese language. KORQ is the only validated keratoconus specific questionnaire and has high rating for psychometric properties.Methods: In this cross-sectional study enrolled 100 patients with keratoconus from a tertiary referral eye hospital, from April 2018 to June 2019. Associations between age, gender, allergic conjunctivitis, keratoconus stage, best-corrected visual acuity (BCVA), maximum simulated keratometry (Kmax), steep keratometry (K2), pachymetry, treatments performed, hydrops and KORQ scores were evaluated with univariate (Wilcoxon test and the Kruskal Wallis test) and multivariate linear regression with stepwise backward modeling. Lower scores of KORQ are associated with better quality of life, as well as higher scores are associated with greater impairment of functional activities and symptoms.Results: Out of the 100 patients, mild, moderate and severe keratoconus, was observed in 15%, 46% and 39% of participants, respectively. Univariate analysis showed lower values for function scores with male gender (p<0.05) and both functional and symptoms scores statistically associated with BCVA<0.3 (LogMAR) (p<0.05). Multivariate analysis indicated significantly lower functional scores in individuals with BCVA<0.3 (p<0.001) and those with history of crosslinking treatment (p=0.022), while symptom scores were only statistically associated with BCVA<0.3 (p<0.001).Conclusions: In patients with keratoconus, BCVA in the better eye and history of crosslinkig are factors were associated with better quality of life scores using KORQ.
The microRNA miR-18a Links Proliferation and Inflammation During Photoreceptor Regeneration in the Injured Zebrafish Retina
Authors: Magner Evin , Sandoval-Sanchez Pamela , Hitchcock Peter F , Taylor Scott M 
Published: April 2021

Abstract
In mammals, photoreceptor loss causes permanent blindness, but in zebrafish (Danio rerio), Müller glia function as intrinsic stem cells, producing progenitor cells that regenerate photoreceptors and restore vision. MicroRNAs (miRNAs) critically regulate neurogenesis in the brain and retina, but the roles of miRNAs in injury-induced neuronal regeneration are largely unknown. The miRNA miR-18a regulates photoreceptor differentiation in the embryonic retina. The purpose of the current study was to determine the function of miR-18a during injury-induced photoreceptor regeneration. RT-qPCR, in-situ hybridization (ISH) and immunohistochemistry (IHC) showed that miR-18a expression increases throughout the retina by 1-day post-injury (dpi) and continues to increase through 5 dpi. Bromodeoxyuridine (BrdU) labeling showed that at 7 and 10 dpi, when regenerated photoreceptors are normally differentiating, there are more proliferating Müller glia-derived progenitors in homozygous miR-18a mutant (miR-18ami5012) retinas compared with wild type (WT), indicating that miR-18a negatively regulates injury-induced proliferation. At 7 and 10 dpi, miR-18ami5012 retinas have fewer mature photoreceptors than WT, but there is no difference at 14 dpi, revealing that photoreceptor regeneration is delayed. BrdU labeling showed that the excess progenitors in miR-18ami5012 retinas migrate to other retinal layers besides the photoreceptor layer. Inflammation is critical for photoreceptor regeneration and RT-qPCR showed that, in the absence of miR-18a, inflammation is prolonged. Suppressing inflammation with dexamethasone rescues the miR-18ami5012 phenotype. Together, these data show that during injury-induced photoreceptor regeneration, miR-18a regulates proliferation and photoreceptor regeneration by regulating key aspects of the inflammatory response during photoreceptor regeneration in zebrafish.
Der Hochschullehrgang Coding und Robotik für Lehrkräfte an der Pädagogischen Hochschule Burgenland
Authors: Leitgeb Thomas , Zimmermann Alexander , Rollett Wolfram 
Published: April 2021

Um an einer zunehmend digitalisierten Welt beruflich und gesellschaftlich teilnehmen zu können, bedarf es umfangreicher und tiefer Kenntnisse bezüglich informatischer Konzepte. Vor diesem Hintergrund erscheint eine Erweiterung des herkömmlichen schulischen Curriculums um die systematische Implementierung von Computational Thinking (CT) –also dem Lösen von Problemen bzw. Aufgaben mit Hilfe algorithmischer Lösungsstrategien unter Rückgriff auf grundlegende Konzepte der Informatik (Wing 2006, 33) – sinnvoll und notwendig. Weltweit wurde dies bereits in vielen Ländern umgesetzt (Bocconi u. a. 2016). Der Lehrkräfteprofessionalisierung wird für die erfolgreiche Implementation von CT in der Schule eine besondere Bedeutung zugesprochen (Pollak und Ebner 2019). Im österreichischen Burgenland wurde zur Förderung von CT für die Primarstufe eine integrative Lehrplanverankerung vorgenommen und für die Sekundarstufe I das Wahlpflichtfach Coding und Robotik eingeführt (Leitgeb 2019). Um die Lehrkräfte entsprechend fort- bzw. weiterbilden zu können, wurde an der Pädagogischen Hochschule Burgenland ein zweijähriger Hochschullehrgang (HLG) Coding und Robotik entwickelt und implementiert. Im vorliegenden Beitrag werden der theoretische Rahmen und Hintergrund, die theoretische Konzeption, die inhaltliche und didaktische Ausgestaltung sowie die Ziele dieses HLG vorgestellt. Ferner werden Ergebnisse einer nach dem ersten Studienjahr durchgeführten Begleitevaluation präsentiert.
Effects of water-saving pruning on the growth and water consumption of jujube (Ziziphus jujuba Mill.) in a loess hilly region with deep soil desiccation
Authors: Ma Jianpeng , Wang Xing , Zhao Xining , Zhang Wenfei , Wang Youke 
Published: April 2021

Abstract
In order to study whether jujube trees can grow normally under rain-fed conditions in loess hilly areas, we planted jujube trees (Ziziphus jujuba Mill.) 4 years after felling a 23-year-old apple orchard. The growth process of the jujube trees and the variation in soil water content (SWC) were monitored for three consecutive years following planting in order to study the effects of the water-saving pruning (WSP) technique. Results showed the following. (1) The soil at a depth of 0–1,000 cm had been desiccated when the area was an apple orchard. (2) Under rain-fed conditions, the jujube trees with the WSP technique were always able to maintain normal growth while the jujube trees with conventional pruning method had a normal growth stage of only 4 years. The water use efficiency of the jujube trees with the WSP technique was much higher than that of the jujube trees with conventional pruning. We recommend WSP in jujube orchard management, because the jujube trees with WSP could maintain normal growth in the deep dried soils of the loess hilly region, as WSP can reduce the water consumption of the jujube trees and may have a positive effect on soil moisture restoration.
Review of 10,11-Dehydrocurvularin: Synthesis, Structural Diversity,
Bioactivities and Mechanisms
Authors: Zhou FuGui , Zhou Yiqing , Guo ZhiYong , Yu XianJun , Deng Zhangshuang 
Published: April 2021

Abstract:
10,11-Dehydrocurvularin is a natural benzenediol lactone (BDL) with a 12-membered macrolide
fused to a resorcinol ring produced as a secondary metabolite by many fungi. In this review, we
summarized the pieces of literature regarding biosynthesis, chemical synthesis, biological activities,
and assumed work mechanisms of 10,11-dehydrocurvularin, which presented a potential for agricultural
and pharmaceutical uses.
QUO VADIS GRANDES ECOLES: ECOLE NATIONALE D’ADMINISTRATION (ENA)
Authors: Wicaksono Kristian Widya 
Published: April 2021

In France, to reach a good career as the high-rank of government officials, people must join the Ecole National d’Administration (ENA). This situation put the ENA graduates (enarques) in the strategic position as the administrative elite but this special privilege gives the word egalité within higher education a hollow sound. This paper wants to know the impact of Ernarques on the merit system recruitments of high-rank civil servants. The method used in this paper writing is a systematic literature review. The results of this study show that it is not compulsory to dissolve ENA but we should treat ENA as an ordinary school for Public Administrator. Then, it is important to create another university that has the same concentration and access as ENA to procreate a fair competition. Finally, we must invite diverse graduates to join the high-rank position to encourage innovation and competitive nuance in the public organization.
MICRO, SMALL, AND MEDIUM ENTERPRISES (MSMEs) EMPOWERMENT THROUGH QUADRUPLE HELIX APPROACH IN BATU CITY
Authors: Mindarti Lely Indah , Muzaqi Ajie Hanif 
Published: April 2021

The research purpose is to describe implementing of MSMEs empowerment in Batu using perspective of Quadruple helix model . This research using descriptive qualitative approach. Government of Batu through Department of Micro Enterprise Cooperatives and Trade of Batu implements MSMEs empowerment by relying on 4 main factors of empowerment: financial capital access, human resource, partnership, and market strengthening. This government also established Integrated Business Service Centre (IBSC) as one of the commitments in empowering MSMEs and also making Batu as MSMEs centre. The result of analysis using the concept of Quadruple helix model found that MSMEs empowerment in Batu involves collaboration of different sectors: the government of Batu, private/business institutions, academics, and civil society through communities, groups, and non-governmental organizations. Thus, the government of Batu plays dominant roles in the interaction synergies of different sectors and being responsible in equitable economic development. Private institutions play roles as production developer, academics play roles as knowledge and skill development transferor and civil society plays roles as creative economic agents and product users