bluuebunny/crossref_metadata_2025_split huggingface dataset:
Development of an LC-MS/MS Method for Measurement of Irinotecan and Its Major Metabolites in Plasma: Technical Considerations
Authors: Aoullay Zineb , Van Wijk Xander M R , Yanhui Ma , Meddah Bouchra , Lynch Kara L , Cherrah Yahia , Wu Alan H B 
Published: June 2021

Abstract

Objective
Irinotecan (CPT-11) is an important drug used in the treatment of several solid tumor types. To minimize its toxicity, therapeutic drug monitoring of CPT-11 and its major metabolites (SN-38, SN-38-glucuronide [SN-38G], and APC) has been proposed. We aimed to develop a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the quantification of CPT-11 and its major metabolites in plasma.

Methods
Specimen preparation consisted of protein precipitation, evaporation, and reconstitution. Analyses were performed on a C18 column using reverse-phase gradient elution. Electrospray ionization and multiple reaction monitoring in positive mode were used for MS. The following heavy isotope–labeled internal standards were used: CPT-11 D10, SN-38 D3, SN-38G D3, and APC D3.

Results
We found that CPT-11, SN-38G, and APC eluted at ~4.6 to 4.7 minutes, and SN-38 eluted at ~5.1 to 5.2 minutes. A second peak for SN-38 was detected at ~4.6 to 4.7 minutes. Given that the structure of SN-38 is found in CPT-11, SN-38G, and APC, and in the CPT-11 D10 used here, in-source fragmentation was the likely cause. In addition, we found that a low-level SN-38 impurity was present in CPT-11 D10 and to a lesser extent in SN-38 D3.

Conclusion
When developing methods for CPT-11 and its metabolites, it is important to consider the effects of in-source fragmentation and the choice of internal standards.
Influence of the menopausal transition on polysomnographic sleep characteristics: a longitudinal analysis
Authors: Matthews Karen A , Lee Laisze , Kravitz Howard M , Joffe Hadine , Neal-Perry Genevieve , Swanson Leslie M , Evans Marissa A , Hall Martica H 
Published: June 2021

AbstractStudy ObjectivesTo evaluate how change in menopausal status related to spectral analysis and polysomnographic measures of sleep characteristics.MethodsThe Study of Women’s Health Across the Nation (SWAN) Ancillary Sleep Study evaluated sleep characteristics of 159 women who were initially pre- or early perimenopausal and repeated the assessment about 3½ years later when 38 were pre- or early perimenopausal, 31 late perimenopausal, and 90 postmenopausal. Participants underwent in-home ambulatory polysomnography for two to three nights. Average EEG power in the delta and beta frequency bands was calculated during NREM and REM sleep, and sleep duration, wake after sleep onset (WASO), and apnea hypopnea index (AHI) were based on visually-scored sleep.ResultsThe women who transitioned to postmenopause had increased beta NREM EEG power at the second assessment, compared to women who remained pre-or early premenopausal; no other sleep measures varied by change in menopausal status. In multivariate models the associations remained; statistical controls for self-reported hot flashes did not explain findings. In secondary analysis, NREM beta power at the second assessment was greater among women who transitioned into the postmenopause after adjustments for initial NREM beta power.ConclusionsSleep duration and WASO did not vary by menopause transition group across assessments. Consistent with prior cross-sectional analysis, elevated beta EEG power in NREM sleep was apparent among women who transitioned to postmenopause, suggesting that independent of self-reported hot flashes, the menopausal transition is associated with physiological hyperarousal during sleep.
Inclusive and Included? Practices of Civic Inclusivity of American Muslims in Los Angeles
Authors: Cantori Valentina 
Published: June 2021

AbstractHow do American Muslims practice inclusivity and bridge religious differences in U.S. civic life? Sociological research on bridging focuses mostly on bridging efforts on the part of majority groups, leaving unanswered the timely question of if and how inclusivity is practiced by minority groups, particularly religious minorities, in U.S. civic spaces. Drawing on participant observation among two Muslim groups in Los Angeles, this paper identifies two practices of inclusivity that participants adopt to bridge religious difference: the interreligious heritage practice and the shared ethics practice. Both practices simultaneously draw and diffuse group boundaries, emphasize sameness, albeit using different sets of religious meanings, and are grounded in an understanding of civic spaces as implicitly exclusionary of minorities. I find that these practices can create tension points in the pursuit of mutual understanding and create textures of meanings that operate differently depending on the situation and the participants in the interaction.
Woodsmoke Extracts Cross-Link Proteins and Induce Cornified Envelope Formation without Stimulating Keratinocyte Terminal Differentiation
Authors: Lin Lo-Wei , Denison Michael S , Rice Robert H 
Published: June 2021

Abstract
Air pollution poses a serious risk to human health. To help understand the contribution of smoke from wood burning to the harmfulness of air pollution toward the skin, we studied the effects of liquid smoke, aqueous extracts of wood smoke condensate, a commercially available food flavor additive, in cultured keratinocytes. We report that liquid smoke can react with and cross-link keratinocyte cellular proteins, leading to abnormal cross-linked envelope formation. Instead of inducing genes ordinarily involved in terminal differentiation, liquid smoke induced expression of genes associated with stress responses. When transglutaminase activity was inhibited, liquid smoke still promoted protein cross-linking and envelope formation in keratinocytes. This phenomenon likely results from oxidative stress and protein adducts from aldehydes as either preloading the cells with N-acetylcysteine or reducing the aldehyde content of liquid smoke decreased its ability to promote protein cross-linking and envelope formation. Finally, liquid smoke-induced envelopes were found to have elevated protein content, suggesting oxidative cross-linking and formation of protein adducts might impair barrier function by inducing abnormal incorporation of cellular proteins into envelopes. Since the cross-linked protein envelope provides structural stability to the stratum corneum and serves as a scaffold for the organization of the corneocyte lipid envelope (hydrophobic barrier to the environment), these findings provide new insight into the mechanism by which pro-oxidative air pollutants can impair epidermal function.
Hormones and carbohydrates are both involved in the negative effects of reproduction on vegetative bud outgrowth in the mango tree: consequences for irregular bearing
Authors: Capelli Mathilde , Lauri Pierre-Éric , Léchaudel Mathieu , Normand Frédéric 
Published: June 2021

AbstractThe negative effects of fruit production during one cycle on reproduction during the following cycle are generally explained by two complementary processes: hormone synthesis and carbohydrate mobilization. Our study focused on mango (Mangifera indica L.) for which it has been shown that reproduction decreases and delays vegetative bud outgrowth. This, in turn, affects flowering and fruiting in the following cycle. Vegetative growth therefore plays a pivotal role in irregular fruit production patterns across consecutive years. Our aim was to decipher the respective roles of hormones and carbohydrates on the negative effects of reproduction on vegetative growth. We analyzed the changes in various hormone (auxin, cytokinin, abscisic acid) and carbohydrate (glucose, sucrose, starch) concentrations in terminal axes with vegetative and reproductive fates of two mango cultivars, Cogshall and José, characterized by different bearing patterns, across consecutive phenological periods during a growing cycle. Auxin concentrations were high in inflorescences, fruit peduncles and axes bearing inflorescences or fruit, suggesting auxin-induced inhibition of vegetative bud outgrowth in the flowering and fruiting axes. Moreover, growing fruits, which are strong sink organs, depleted carbohydrates from non-fruiting axes. During vegetative growth, this starch depletion probably contributed to decreasing the probability of and to delaying vegetative bud outgrowth of reproductive axes for Cogshall, and of reproductive and nonreproductive axes for José. Starch dynamics in quiescent and flowering growth units during early fruit growth and their starch concentrations at fruit maturity differed between the two cultivars, presumably in relation to the observed contrasted crop loads and/or to differences in photosynthetic capacity or carbohydrate allocation. These differences between the two cultivars in terms of starch concentration in terminal axes during vegetative growth could partly explain their different bearing patterns.
PtrMYB120 functions as a positive regulator of both anthocyanin and lignin biosynthetic pathway in a hybrid poplar
Authors: Kim Min-Ha , Cho Jin-Seong , Bae Eun-Kyung , Choi Young-Im , Eom Seok Hyun , Lim You Jin , Lee Hyoshin , Park Eung-Jun , Ko Jae-Heung 
Published: June 2021

Abstract
Both anthocyanins and lignins are essential secondary metabolites in plant growth and development. Their biosynthesis is metabolically interconnected and diverges in the central metabolite 4-coumaroyl CoA of the phenylpropanoid pathway. Considerable progress has been made in understanding transcriptional regulation of genes involved in lignin and anthocyanin synthesis pathways, but the concerted regulation of these pathways is not yet fully understood. Here, we functionally characterized PtrMYB120, a R2R3-MYB transcription factor from Populus trichocarpa. Overexpression of PtrMYB120 in a hybrid poplar (i.e., 35S::PtrMYB120) was associated with increased anthocyanin (i.e., cyanidin 3-O-glucoside) accumulation and upregulation of anthocyanin biosynthetic genes. However, transgenic poplars with dominant suppression of PtrMYB120 function achieved by fusing the ERF-associated amphiphilic repression motif to PtrMYB120 (i.e., 35S::PtrMYB120-SRDX) had a dramatic decrease in not only anthocyanin but also Klason lignin content with downregulation of both anthocyanin and lignin biosynthetic genes. Indeed, 35S::PtrMYB120-SRDX poplars had irregularly shaped xylem vessels with reduced S-lignin content in stems, which was proportionally related to the level of the introduced PtrMYB120-SRDX gene. Furthermore, protoplast-based transcriptional activation assay using the PtrMYB120-GR system suggested that PtrMYB120 directly regulates genes involved in both anthocyanin and lignin biosynthesis, including chalcone synthase and ferulate-5 hydroxylase. Interestingly, the saccharification efficiency of line #6 of 35S::PtrMYB120-SRDX poplars, which had slightly reduced lignin content with a normal growth phenotype, was dramatically enhanced (&gt;45%) by NaOH treatment. Taken together, our results suggest that PtrMYB120 functions as a positive regulator of both anthocyanin and lignin biosynthetic pathways and can be targeted to enhance saccharification efficiency in woody perennials.
A comprehensive analysis of genetic diversity of EBV reveals potential high-risk subtypes associated with nasopharyngeal carcinoma in China
Authors: Xue Wen-Qiong , Wang Tong-Min , Huang Jing-Wen , Zhang Jiang-Bo , He Yong-Qiao , Wu Zi-Yi , Liao Ying , Yuan Lei-Lei , Mu Jianbing , Jia Wei-Hua 
Published: June 2021

Abstract
Epstein–Barr virus (EBV), a widespread oncovirus, is associated with multiple cancers including nasopharyngeal carcinoma (NPC), gastric cancer and diverse lymphoid malignancies. Recent studies reveal that specific EBV strains or subtypes are associated with NPC development in endemic regions. However, these NPC specific subtypes were only identified in a portion of infected individuals due possibly to the limited samples size studied or the complicated population structures of the virus. To identify additional high-risk EBV subtypes, we conducted a comprehensive genetic analysis of 22 critical viral proteins by using the largest dataset of 628 EBV genomes and 792 sequences of single target genes/proteins from GenBank. The phylogenetic, principal component and genetic structure analyses of these viral proteins were performed through worldwide populations. In addition to the general Asia-Western/Africa geographic segregation, population structure analysis showed a ‘Chinese-unique’ cluster (96.57% isolates from China) was highly enriched in the NPC patients, compared to the healthy individuals (89.6% vs. 44.5%, P &lt; 0.001). The newly identified EBV subtypes, which contains four Chinese-specific NPC-associated amino acid substitutions (BALF2 V317M, BNRF1 G696R, V1222I and RPMS1 D51E), showed a robust positive association with the risk of NPC in China (Odds Ratio = 4.80, 20.00, 18.24 and 32.00 for 1, 2, 3 and 4 substitutions, respectively, Ptrend &lt;0.001). Interestingly, the coincidence of positively selected sites with NPC-associated substitutions suggests that adaptive nonsynonymous mutation on critical proteins, such as BNRF1, may interact with host immune system and contribute to the carcinogenesis of NPC. Our findings provide a comprehensive overview of EBV genetic structure for worldwide populations and offer novel clues to EBV carcinogenesis from the aspect of evolution.
Transition to Practice and Back Again: The Journey Into Nursing Academia
Authors: Ruiz Diana , Gonzales Minerva 
Published: June 2021

Abstract
There is little research on the transition of experienced nurses from acute/clinical practice to academia. The transition into a faculty role can be overwhelming, isolating, and intimidating, even for expert nurse leaders and clinicians. This article presents the journey of two experienced nurse leaders who transitioned into academia and explores how to overcome challenges during the transition period.
Validated Outcome Measures and Postsurgical Scar Assessment Instruments in Auricular Surgery: A Systematic Review
Authors: Chu Sherman , Ramanathan Diya , Thuener Jason E. , Carroll Bryan T. 
Published: June 2021

BACKGROUND
The utilization of an assessment instrument that successfully analyzes validated outcome measures for auricular surgery is important for advancing evidence-based medicine.

OBJECTIVE
To systematically review postsurgical scar assessment instruments and outcome measures after auricular surgery to assess if any individual or combination of 2 assessment instruments encompass all relevant, validated auricular outcome measures.

METHODS
Two systematic reviews were conducted using the PubMed/MEDLINE and Ovid databases: one for postauricular surgical outcome measures and another for postsurgical scar assessment instruments. Auricular outcome measure articles were selected for inclusion if they included at least one auricular-specific validated outcome measure, and assessment tool articles were included if they referenced one or more specific tool(s) specifically designed to assess postsurgical scars. Assessment tools were evaluated based on which outcome measures each covered.

RESULTS
There was no single postsurgical scar assessment instrument or combination of 2 instruments that covered all outcome measures within the 5 different categories (psychosocial well-being, functional, objective appearance, subjective appearance, and clinical-related outcomes) after auricular surgery. None of the instruments measured functional outcomes, such as the ability to wear glasses and hearing outcomes.

CONCLUSION
There is currently no existing postsurgical scar assessment instrument that covers all outcome measures after auricular surgery.
Peak Efficiency of NaI Detector and Coincidence Summing Factor for Different Cylindrical Sources Using Geant4 Simulation
Authors: Alzahrani Jamila S. , Almuqrin Aljawhara H. 
Published: June 2021

Abstract
Geant4 simulation is used to calculate peak efficiency and correct the effect of coincidence summing in detecting volumetric gamma-ray sources; this simulation was applied to a standard 152Eu source with different volumes as a test case. The source is a liquid cylindrical shape of various volumes. Peak efficiency was calculated using two tracks in the Geant4 simulation: single-energy track and “monoenergetic Track” without coincidence summing. Here, the energy of the source is known, and the track of radionuclides, including the coincidence summing, depends on the decay scheme of the radioactive source. The ratio between the peak efficiency of the two tracks gives us the correction factor (CF). The experimental method was used to calculate the peak efficiency and was amended by the correction factor computed with Geant4 tracks. The results showed a good agreement between experimental efficiency after correction and free-summing simulated efficiency. The comparison indicated that the present method is valid and useful for voluminous gamma-ray sources.
Clinical significance of olfactory dysfunction in patients of COVID-19
Authors: Chang Chin-Chuan , Yang Ming-Hui , Chang Shu-Min , Hsieh Ya-Ju , Lee Che-Hsin , Chen Yi-Ming Arthur , Yuan Cheng-Hui , Chen Yi-Ling , Ho Sheng-Yow , Tyan Yu-Chang 
Published: June 2021

Background:
Currently, as the coronavirus disease (COVID-19) has become a pandemic, rapidly obtaining accurate information of patient symptoms and their progression is crucial and vital. Although the early studies in China have illustrated that the representative symptoms of COVID-19 include (dry) cough, fever, headache, fatigue, gastrointestinal discomfort, dyspnea, and muscle pain, there is increasing evidence to suggest that olfactory and taste disorder are related to the COVID-19 pandemic. Therefore, we conduct this study to review the present literature about the correlation between anosmia or dysgeusia and COVID-19.

Methods:
A comprehensive literature search in 2020 of the electronic journal databases, mainly PubMed or Web of Science, was performed using the keywords COVID-19 or severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), with hyposmia, anosmia, dysgeusia, olfactory disorder, or olfactory dysfunction. The country, study period, case number, inpatient or outpatient medical visit, evaluation method (subjective complaints of dysfunction or objective evaluation), and occurrence rate of olfactory or gustatory function were reviewed.

Results:
Many studies reported that the recoverable olfactory or gustatory dysfunction may play an important role as the early clinical symptom of COVID-19. It is associated with better prognosis, although further investigation and validation should be carried out.

Conclusion:
Studies have shown that smell and taste disturbances may represent an early symptom of COVID-19 and healthcare professionals must be very vigilant when managing patients with these symptoms. In the pandemic era, this implies testing for COVID-19 by healthcare workers with full personal protective equipment.
The Effect of the Neuman Systems Model on Anxiety in Patients Undergoing Coronary Artery Bypass Graft: A Randomized Controlled Trial
Authors: AKHLAGHI Elham , BABAEI Sima , MARDANI Abbas , ESKANDARI Fatemeh 
Published: June 2021

ABSTRACT

Background
Coronary artery bypass graft (CABG) surgery is the most commonly used surgical procedure for patients with coronary artery disease. Anxiety is a significant psychological problem associated with CABG surgery that may reduce related treatment outcomes.

Purpose
This study was designed to investigate the effect on anxiety of applying the Neuman Systems Model in patients awaiting and undergoing CABG surgery.

Methods
A single-blind, parallel-group randomized controlled trial was conducted on patients scheduled to receive coronary artery bypass surgery in an urban area of Iran. Seventy participants were enrolled using a convenience sampling method and randomly assigned to either the intervention group (n = 35) or the control group (n = 35). Participants in the intervention group received intervention in accordance with the Neuman Systems Model format, including meetings held at three levels of prevention. Patients' anxiety was measured using the State Anxiety Inventory before the intervention, immediately after the intervention, and at discharge time. The chi-square test, Fisher's exact test, independent sample t test, one-way repeated measures analysis of variance test, and Fisher's least significant difference were used to analyze the data.

Results
No significant difference was found between the mean anxiety scores of the two groups before the intervention (p = .48, t = 0.71). However, the mean anxiety score of the intervention group was significantly lower than that of the control group immediately after the intervention (p = .008, t = 2.73) and at discharge time (p = .007, t = 2.77).

Conclusions
The Neuman-based program is an effective and low-cost intervention that may be applied to reduce anxiety in patients awaiting and undergoing CABG surgery. This program may be a good guide in providing healthcare services.
A Health Surveillance Study of Workers Employed at a Copper Smelter—Effects of Long-Term Exposure to Copper on Lung Function Using Spirometric Data
Authors: Haase Lisa-Marie , Birk Thomas , Bachand Annette M. , Mundt Kenneth A. 
Published: June 2021

Objective:
Significantly lower permissible occupational exposure limits for copper dust are being discussed in Europe and other jurisdictions. However, little data are published on exposures in occupational settings and copper-specific effects in humans. Hence, a health surveillance study was performed among workers employed at a copper smelter between 1972 and 2018.

Methods:
Possible effects of long-term exposures to dust containing copper on lung function were assessed. Specifically, declines in forced expiratory volume in 1 second (FEV1) were compared between a copper-exposed and control group. Cumulative copper exposures were derived from historical airborne monitoring data.

Results:
FEV1 declines among exposed and control never smokers were similar to a typical age-dependent decline of 29 mL/y.

Conclusion:
The study findings indicate that cumulative inhalable copper dust exposure averaging 4.61 mg/m3-years over an exposure duration of ∼22 years is not associated with adverse effects on lung function.