bluuebunny/crossref_metadata_2025_split huggingface dataset:
LI-RADS Classification and Outcomes of Hepatocellular Carcinoma Treated with Transcatheter Arterial Chemoembolization  Combined with Radiofrequency Ablation
Authors: Tachibana Yuji , Takaji Ryo , Maruno Miyuki , Honda Koichi , Endo Mizuki , Murakami Kazunari , Asayama Yoshiki 
Published: December 2021

Abstract
PurposeThe aim of this study was to clarify the usefulness of the Liver Imaging Reporting and Data System (LI-RADS) to predict the patients’ prognosis after transcatheter arterial chemoembolization (TACE) combined with radiofrequency ablation (TACE-RFA) for hepatocellular carcinoma (HCC) of Barcelona-Clinic Liver Cancer Stage (BCLC) 0 or A.Materials and MethodsWe retrospectively analyzed cases of HCC patients who underwent TACE-RFA (Jan 2005 to Dec 2015). Patients’ nodules were categorized based on their LI-RADS v2018 core. The LI-RADS category was assigned to each nodule using dynamic contrast-enhanced CT. LR-3, LR-4 and LR-5 nodules were extracted. We assessed the overall survival (OS) and recurrence-free survival (RFS) among BCLC 0 and BCLC A patients.ResultsOf the 64 nodules extracted, 22 were LR-3 or -4 (14.8±6.7 mm) and 42 were LR-5 (17.1±6.9 mm). Regarding OS, there was no significant difference between LR-3 or -4 and LR-5 (p=0.278). In terms of RFS, there was a significant difference between LR-3 or -4 and LR-5 (p=0.03). In particular, patients with BCLC A with LR-5 nodules showed significantly poorer RFS than those with LR-3 or -4 (p=0.016) nodules. Conclusions﻿For patients with BCLC A, LR-3 or -4 are associated with a better prognosis than LR-5 nodules.
Reduced Risk of Serious Pneumococcal Infection Up to 10 Years After 7-Valent Pneumococcal Conjugate Vaccine in Arthritis Patients
Authors: Nagel Johanna , Jönsson Göran , Nilsson Jan-Åke , Manuswin Chanchai , Englund Martin , Saxne Tore , Kapetanovic Meliha C 
Published: December 2021

Abstract
Background: To examine rates of serious pneumococcal infections up to 10 years after vaccination with 7-valent conjugated pneumococcal vaccine (PCV7) in patients with arthritis compared to non-vaccinated arthritis patients.Methods: In total, 595 adult arthritis patients (rheumatoid arthritis; RA=342, 80% women and spondylarthropathy; SpA=253, 45% women) received one dose of PCV7. Mean age/disease duration were 62/16 and 51/14 years, respectively. For each patient, 4 matched reference subjects were identified.At vaccination, 420 patients received bDMARDs (anti-TNF=330, tocilizumab=15, abatacept=18, anakinra=1, rituximab=56). Methotrexate was given as monotherapy (n=86) or in combination with bDMARD (n=220). 89 SpA patients received NSAIDs without DMARD. The Skåne Healthcare Register was searched for ICD-10 diagnostic codes for pneumococcal infections (pneumonia, lower respiratory tract infection, septicemia, meningitis, septic arthritis) between January 2000 and December 2018. Frequency of infections after vs before vaccination were calculated (relative risks). Relative risk ratio (RRR) and relative risk reduction (1-RRR) were calculated comparing patients vs non-vaccinated references. Kaplan-Meier and Cox regression were used to investigate time to first event and predictors of infections.Results: Among vaccinated RA and SpA patients, there was a significant relative risk reduction of pneumonia and all serious infections; 53% and 46%, respectively. There was no significant difference in time to first pneumonia or all serious infections after vaccination between patients and references. Higher age, RA diagnosis and concomitant prednisolone were associated with infections.Conclusion: One dose of pneumococcal conjugate vaccine may decrease risk of serious pneumococcal infection up to 10 years in patients with arthritis receiving immunomodulating treatment. Clinical trial registration number: EudraCT EU 2007-006539-29 and NCT 00828997
Phyllantus Emblica Mediated Silica Nanomaterials: Biosynthesis, Structural and Stability Analysis
Authors: Sankareswaran M , Vanitha M , Rajiv P. , Anbukumaran A. 
Published: December 2021

Abstract
The current investigation reports on a green route, simple and eco-friendly method for synthesis of silica nanoparticles from Phyllantus emblica. Appropriate characterization techniques were employed to assess the crystalline nature, microstructure, size, purity, elemental composition and stability of as-biosynthesized silica nanoparticles. The XRD analysis showed a wide-ranging peak at 22∘ of 2θ value and proved that the nanoparticles were crystalline nature with 32 nm average size of particles. FT-IR studies confirmed the occurrence of metal oxide group and presence of phyto-molecules namely hydroxyl, amide, and carboxyl functional groups, which were responsible for formation and stabilization of silica nanomaterials. TGA and Zeta potential analysis determined that silica nanoparticles are highly thermostable. EDX analysis revealed the purity of nanomaterials and spectra confirmed that formation of silica nanomaterials (72.97 weight percentage of SiO2 content) with low impurities. SEM analysis shows that the particles are spherical in shape with low agglomeration. This research work concluded that the P. emblica was an excellent and reliable green resource for production of highly stable and potential silica nanoparticles.
Angiotensin-(3–4) (Val-Tyr) Normalizes the Elevated Arterial Blood Pressure and Abnormal Na+/Energy Handling Associated With Chronic Undernutrition by Counteracting the Effects Mediated by Type 1 Angiotensin II Receptors
Authors: Pereira-Acacio Amaury , Veloso-Santos João , Nossar Luiz , Costa-Sarmento Gloria , Muzi-Filho Humberto , Vieyra Adalberto 
Published: December 2021

Abstract
Purpose To investigate the mechanisms by which chronic administration of a multideficient diet after weaning alters bodily Na+ handling, and culminates in high systolic blood pressure (SBP) at a juvenile age.Methods From 28 to 93 days of age, weaned male Wistar rats were given a diet with low content and poor-quality protein, low lipid, without vitamin supplementation, which mimics the diets consumed in impoverished regions worldwide. We measured food, energy and Na+ ingestion, together with urinary Na+ excretion, Na+ density (Na+ intake/energy intake), plasma Na+ concentration, SBP), and renal proximal tubule Na+-transporting ATPases. Results Undernourished rats aged 93 days had only one-third of the control body mass, lower plasma albumin, higher SBP, higher energy intake, and higher positive Na+ balance accompanied by decreased plasma Na+ concentration. SBP was normalized with Losartan and with Ang-(3–4), and the combination of the 2 substances induced an accentuated negative Na+ balance as a result of strong inhibition of Na+ ingestion. Na+ density in undernourished rats was higher than in control, irrespective of the treatment, and they had downregulated (Na++K+)ATPase and upregulated Na+-ATPase in proximal tubule cells, which returned to control levels after Losartan or Ang-(3–4).Conclusions Na+ density, not only Na+ ingestion, plays a central role in the pathophysiology of elevated SBP in chronically undernourished rats. The observations that Losartan and Ang-(3–4) normalized SBP together with Na+ distribution and handling give support to the proposal that Ang IIÞAT1R and Ang IIÞAT2R axes have opposite roles within the renin-angiotensin-aldosterone system of undernourished juvenile rats.
An Observational, Non-Interventional Study for the Follow-up of Patients with Amyloidosis Who Received Miridesap Followed by Dezamizumab in a Phase 1 Study
Authors: Richards Duncan , Millns Helen , Cookson Louise , Lukas Mary Ann 
Published: December 2021

Abstract
Background: Miridesap depletes circulating serum amyloid P (SAP) and dezamizumab (anti-SAP monoclonal antibody) targets SAP on amyloid deposits, triggering amyloid removal. In a Phase 1, first-in-human study (FIHS), progressive amyloid removal was observed in some patients after ≤3 cycles of miridesap/dezamizumab.Methods: This observational, non-interventional study in patients who received miridesap/dezamizumab during the FIHS (planned follow-up: 5 years) evaluated response to treatment based on routine assessments of disease status and key organ function. In a post hoc analysis, patients responding to treatment in the FIHS during follow-up were identified as responders and further categorized as sustained or declining responders.Results: In the FIHS, 17/23 patients were treatment responders. Of these patients, seven (immunoglobulin light chain [AL], n=6; serum amyloid A, n=1) were considered sustained responders and ten (fibrinogen-a alpha chain [AFib], n=5; AL, n=4; apolipoprotein A-I, n=1) were considered declining responders. We primarily present responder patient-level data for functional, cardiac, laboratory and imaging assessments conducted during the follow-up period, with non-responder data presented as supplementary.Conclusion: No further development of miridesap/dezamizumab is planned in amyloidosis. However, long-term follow-up of these patients may provide insight into whether active removal of amyloid deposits has an impact on disease progression. Trial registration: ClinicalTrials.gov, NCT01777243. Registered 28 January 2013, https://clinicaltrials.gov/ct2/show/study/NCT01777243.
“Pain Management in Intensive Care Patients, a Retrospective Observational Research.”
Authors: Dinse Christoph Moritz , Bucher Michael , Burgdorff Anna-Maria , Christel Annett , Flöther Lilit 
Published: December 2021

Abstract
Background
Sepsis and septic shock are the most common causes of death in non-cardiac surgery intensive care units. The treatment of sepsis is difficult. Adequate analgesia is essential for a positive outcome. There are differences in pain management between septic and non-septic patients. Septic ICU patients receive higher doses of opioids than non-septic ICU patients.
Methods
A retrospective observational study was carried out in an anesthesiological intensive care from 1.1.2014 to 30.6.2016. The cases were divided into four different groups according to the criteria: Sepsis “yes/no” and communication ability “yes/no”. After adjusting the number of cases by pairing method, 356 cases were recruited. The endpoint of our study was defined as the "total opioid dose". A statistical evaluation was carried out by T-tests and two-factor variance analyses.
Results
There is a significant difference in opioid dose between communicative and non-communicative ICU patients. The mean sufentanil dose is significantly higher in the non-communicative patients than in the communicative patients. Sepsis has no significant effect on the dose of sufentanil.
Conclusion
The hypothesis that sepsis decreases the pain threshold could not be proven in this study. The effect of the higher opioid requirement is not directly caused by sepsis, but by the factor of communication ability. Furthermore, we were able to show through our investigations and especially through the data of the pain recording instruments that the septic and non-septic intensive care patients receive sufficient pain therapy treatment.
Single-Cell RNA Sequencing of Peripheral Blood Reveals That Monocytes With High Cathepsin S Expression Aggravate Cerebral Ischemia-Reperfusion Injury
Authors: Xie Lexing , Zhang Shuang , Huang Li , Peng Zhouzhou , Lu Hui , He Qian , Hu Linlin , Wang Bingqiao , Sun Baoliang , Yang Qingwu , Xie Qi 
Published: December 2021

Abstract
Background: Stroke persists as a major cause of morbidity and mortality worldwide. After a stroke, peripheral immune cells are rapidly activated and then infiltrate the central nervous system to cause inflammation in the brain. However, it is not clear when and how these peripheral immune cells affect the central inflammatory response and whether there are intervention targets that can alleviate ischemia-reperfusion injury. In this study, we collected mouse peripheral blood samples at different time points after stroke for single-cell sequencing to reveal the dynamic changes in peripheral immune cells. Methods: We performed single-cell sequencing on peripheral blood of mice at 1, 3, 7, and 14 days after ischemia-reperfusion to analyze the changes of subpopulations after cerebral ischemia-reperfusion; Real-time PCR, western blot and ﻿enzyme‑linked immunoabsorbent assay were used to perform mRNA and protein levels verification; ﻿Immunoprecipitation verifies the interaction of proteins and between junctional adhesion molecule (JAM-A) and Cathepsin S (CTSS) protein, in vitro enzyme digestion and silver staining method to detect the protease digestion effect of CTSS.Results: Peripheral monocyte subpopulations increased significantly after ischemia-reperfusion. Pseudo-time trajectory analysis and gene function analysis further suggested that CTSS may play an important role in regulating monocyte activation and leading to proteolysis. Next, we found that the expression of CTSS was significantly increased in monocytes after I/R in mice. Then, we used CTSS inhibitors and knockout mouse experiments to prove that inhibiting its expression can significantly reduce infarct volume and reduce blood–brain barrier (BBB) leakage. In addition, we found that CTSS destroys BBB by binding to JAM family proteins to cause them degradation. Conclusion: Inhibition of Cathepsin S attenuated cerebral ischemia reperfusion injury and Cathepsin S can be used as a novel target for drug intervention after stroke.
Clinical Characteristics and Economic Burden of Asthma in China: A Multicenter Retrospective Study
Authors: Shang Bin , Li Xiangguo , Xu Yu , Ren Wenxin , Wang Junren , Jiang Shujuan , Sun Jian 
Published: December 2021

Abstract
Background: Asthma is a common chronic airway inflammation that produces a healthcare burden on the economy.Objective: To obtain a better understanding of the clinical status and disease burden of patients with asthma in China. Methods: A retrospective study based on the computerized medical records in the Jinan Health Medical Big Data Platform between 2011 and 2019 (n = 31,082) was carried out. The asthma severity of each patient was assessed retrospectively and categorized as mild, moderate, or severe according to GINA 2018. Results: The results revealed that the majority (75.0%) of patients suffered from mild asthma. Patients treated with ICS/LABA at emergency department visits had lower frequencies of exacerbations in the following year compared with non-ICS/LABA treated patients.The incidence rates for 1 exacerbation are 14.49% vs 15.01 for the patients treated with ICS/LABA and without. The rates are 11.94% and 19.12% for 2 exacerbations above. The numbers are 6.51% vs 12.92% for 3 exacerbations above. The rates are 4.10% vs 9.35% for 4 exacerbations above. Also the difference got the statistical significance (p <0.001), COPD and GRED, two comorbidities related to asthma, were risk factors for asthma exacerbation. Finally, patients who suffered from exacerbations produced a heavier economic burden compared to the patients who never suffered exacerbations(mean costs are ￥3,339.67 vs ￥968.45 separately). Conclusions: These results provide a reference for clinicians and patients to obtain a better treatment and therapy strategy management for asthma sufferers.
Identification of Enhancer RNA CDK6-AS1 as a Potential Novel Prognostic Biomarker in Gastric Cancer
Authors: Yang Shifeng , Zou Xiaoming , Yang Hao , Li Jiacheng , Zhang Ange , Zhang LIsha , Li Changjian , Zhu Lei , Ma Zhen 
Published: December 2021

Abstract
Background:The aim of this study was to confirm enhancer RNAs (eRNAs) in gastric cancer and its clincial utility. Methods:We used cox survival analysis and relevance analysis to identify the candidate eRNAs in gastric cancer. Moreover, we performed GO and Reactome pathway enrichment to found the potential functions of eRNAs.Correlation between eRNA, tumor-infiltrating immune cells and drug sensitivity was then analyzed. Results: CDK6-AS1 may serve as a poor independent prognostic biomarker candidate in gastric cancer with positive correlation with its target gene CDK6. Low CDK6-AS1 expression group showed more frequent mutated driver genes than high expression ones. Moreover, CDK6-AS1 is involved in key oncogenic pathway as cell cycle and RNA transcription. CDK6-AS1 also shows dysregulations and associations with prognosis at pan-cancer level. This eRNA may also associated with immune cell infiltration and drug sensitivity. Conclusion:CDK6-AS1 may be a potential prognostic biomarker for gastric cancer, predict chemotherapeutic drugs sensitivity of gastric cancer.
A Phosphite-Based Sscreening Platform for Identification of Enzymes Favoring Nonnatural Cofactors
Authors: Liu Yuxue , Li Zhuoya , Guo Xiaojia , Wang Xueying , Zhao Zongbao K. 
Published: December 2021

Abstract
BackgroundEnzymes with dedicated cofactor preference are essential for advanced biocatalysis and biomanufacturing. However, directed evolution of an enzyme to switch its cofactor preference is often hindered by the lack of efficient and affordable method for screening as the cofactor per se or the substrate can be prohibitively expensive. Here, we developed a growth-based selection platform to identify nonnatural cofactor-dependent oxidoreductase mutants.ResultsThe growth-based selection platform was designed by coupling with nonnatural cofactor-dependent phosphite dehydrogenase (Pdh) mediated the conversion of non-metabolizable phosphite into phosphate in the culture media. Thus, Pdh variant that strongly favors nicotinamide cytosine dinucleotide (NCD), a NAD analogue, the feasibility of this strategy was successfully demonstrated using derived NCD-active malic enzyme as well as for the directed evolution of NCD synthetase in Escherichia coli.ConclusionsHere, we built a phosphite-based screening platform for identification of enzymes favoring nonnatural cofactor NCD. In the future, once Pdh variants favoring other biomimetic or nonnatural cofactors are available this selection platform may be readily redesigned to attain new enzyme variants with anticipated cofactor preference, providing opportunities to further expand the chemical space of redox cofactors in chemical biology and synthetic biology.
Ovarian Estrogen Synthesis Reduced by Peritoneal Endometriosis in Rat Autologous Transplantation Model
Authors: Zhang Jian , Zhang Yu , Luo Jiali , Yu Lin , Li Panpan , Wang Xinyue , Zhang Tingting , Sun Zhaogui 
Published: December 2021

Abstract
OBJECTIVE: To investigate the effects of peritoneal endometriosis on rat ovaries. METHODS: A rat model of peritoneal endometriosis was established by autologous transplantation. qPCR was performed to measure mRNA levels of steroid hormone and steroid synthesis-related genes in the ovaries of endometriosis rats. Immunohistochemistry was performed to characterize the distribution of FSHR in the ovaries of endometriosis rats. RNAseq was performed to find pathological changes in the ovaries of endometriosis rats. RESULTS: By qPCR, it was revealed that mRNA levels of steroid hormone synthesis-related genes were decreased in the ovaries of rats with endometriosis; With IHC, observed that FSHR expression was significantly decreased in the antral follicles of rats with endometriosis. RNAseq revealed that endometriosis affected transcription of the genes related to the microtubule structure and tight junctions of rat ovarian cells. CONCLUSION: Peritoneal endometriosis decreased the genic expression of ovarian steroid hormone synthetases and FSHR protein level in granulosa cells of antral follicles, and reduced the mRNA levels of the microtubule structure and tight junctions in rat ovarian cells, which contribute to the impairment of ovarian function.
Changes in Hard and Soft Tissue During the First 6 Months After Immediate Implant Placement and Provisionalization of a Single Anterior Maxillary Incisor: A Prospective Cohort Study
Authors: Wei Donghao , Jiang Xi , Di Ping , Tian Jiehua , Lin Ye 
Published: December 2021

Abstract
Background: To evaluate changes in hard and soft tissue during the first 6 months after immediate implant placement and provisionalization (IIPP) of a single maxillary incisor.Methods: Failed maxillary incisors were replaced with IIP implants in 34 patients. Intraoral scans and cone beam computed tomography (CBCT) were performed before and 6 months after IIPP. Changes in soft tissue thickness, soft tissue contour, and hard tissue contour were measured by a three-dimensional superimposition method. The correlations of hard and soft tissue contour changes were assessed.Results: A total of 31 patients completed the study. Soft tissue contour tended to collapse after 6 months. Soft tissue was significantly thickened 1–3 mm below the gingival margin. Correlation analysis showed strong correlations between the hard and soft tissue contour changes at 0–5 mm apical to the implant platform. The mid-facial recession at 6 months was −0.46 ± 0.55 mm.Conclusions: Soft tissue contour collapsed after tooth extraction, regardless of IIPP. Both soft and hard tissue change affected the soft tissue contour change.
The Morbidity Analysis of 17 Notifiable Bacterial Infectious Diseases in China, 2004-2019
Authors: zhu yuliang , Lin Shenghong , Dong Shuaibing , Zhang Cuihong , Shi Lusha , Ren Xiang , Li Zhongjie , Fang Liqun , wang Liping 
Published: December 2021

Abstract
Background

To better understand the epidemic dynamic of notifiable BIDs in China, and to provide scientific evidence for the prevention and control measures.
Methods

we gathered data from the NIDRIS in China from 2004 to 2019. The methods of descriptive epidemiology were applied to analyze the data of BIDs. The Joinpoint Regression analysis was utilized to examine trends in the incidence rates of BIDs.
Results

During 2004‒2019, the average annual incidence rate reported for notifiable BIDs was 134.00 of 100 000. The overall average annual percent changes (AAPC) for RTDs and DCFTDs was -1.98% and -11.66%, respectively. Both of BSTDs and ZVDs showed increasing trends with AAPCs of 4.74% and 4.46%, respectively. Pertussis and scarlet fever showed the fastest increase of the incidence rate in the age group of 0~5 years with AAPC of 15.17% and 12.05%. RTDs had the highest incidence rate in Northwest China. South and East China represented a higher morbidity in BSTDs. The proportion of laboratory confirmation of BIDs have increased from 43.80–64.04%.
Conclusions

RTDs and DCFTDs showed an overall downward trend in China for a dozen years, while BSTDs and ZVDs indicated significantly upward trends.