bluuebunny/crossref_metadata_2025_split huggingface dataset:
Inflows and river heights in Australia’s Murray Darling Basin: impacts of decreased catchment rainfall on water availability.
Authors: Speer Milton , Leslie Lance , MacNamara Shev , Hartigan Joshua 
Published: February 2021

Abstract
The Murray Darling Basin (MDB) is Australia’s most important agricultural region. The southern MDB receives most of its annual catchment runoff during the cool season (April-September). Focusing on the Murrumbidgee River measurements at Wagga Wagga and further downstream at Hay, river heights in the cool season decreased markedly in variability over a century prior to 1991. Box and whisker plots of 27-year April-September Hay and Wagga Wagga river heights reveal decreases not matched by declining April-September catchment rainfall. However, permutation tests of both means and variances of late autumn (April-May) dam catchment rainfall and inflows, produced p-values indicating a highly significant decline since the early 1990s. Consequently, dry catchments in late autumn, even with average cool season rainfall, have reduced dam inflows and decreased river heights downstream from Wagga Wagga, before water extraction for irrigation. It is concluded that lower April-September mean river heights at Wagga Wagga and decreased river height variability at Hay, since the mid-1990s, are due to combined lesser April-May catchment rainfall and increased mean temperatures. If these drying and warming trends continue, they will drastically reduce water availability for the Murrumbidgee River catchment and, consequently, for a vital part of the southern MDB.
Association between eating habits and clinical outcome in patients with ulcerative colitis: A cross sectional study
Authors: Furukawa Shinya , Yagi Sen , Shiraishi Kana , Hashimoto Yu , Kitahata Shogo , Hanayama Masakazu , Tange Kazuhiro , Mori Kenichiro , Ninomiya Tomoyuki , Suzuki Seiyuu , Shibata Naozumi , Murakami Hidehiro , Ohashi Katsuhisa , Hasebe Aki , Tomida Hideomi , Yamamoto Yasunori , Takeshita Eiji , Ikeda Yoshio , Hiasa Yoichi 
Published: February 2021

Abstract
Background: Although the association between eating habits which can be modified and digestive diseases has been reported, to date, no research has evaluated the association between eating habits and ulcerative colitis (UC). Thus, we investigate the association between eating behavior and clinical outcome in Japanese patients with UC.Methods: Eating quickly, eating until full, and skipping breakfast data was obtained from a self-administered questionnaire. Information on clinical outcome was collected from medical records. Mucosal healing (MH) and partial MH was defined as a Mayo endoscopic subscore of 0 or 0–1, respectively. Age, sex, BMI, current smoking, current drinking, steroid use, and anti-TNFα monoclonal antibody use were selected a priori as potential confounding factors.Results: Study subjects consisted of 294 Japanese patients with UC. Eating at speed moderate and eating quickly were independently inversely associated with MH: the adjusted odds ratios (ORs) were 0.38 (95% confidence interval [CI]: 0.16–0.85) and 0.38 (95% CI: 0.17–0.81) (p for trend = 0.033). Eating until full was independently inversely associated with MH: the adjusted OR was 0.38 (95% CI: 0.27–0.86). MH in patients who skipped breakfast was marginally lower than that in patients who did not skip breakfast. No association between eating habits and clinical remission or partial MH was found.Conclusion: Among patients with UC, eating rate and eating until full may be independently inversely associated with MH but not clinical remission.
Testing Stratified Care for Musculoskeletal Pain in General Practice: Integrating Clinician Support and Intervention Design
Authors: Protheroe Joanne , Saunders Benjamin , Hill Jonathan C , Chudyk Adrian , Foster Nadine E , Bartlam Bernadette , Wathall Simon , Cooper Vincent 
Published: February 2021

Abstract
Background
Stratified care involves subgrouping patients based on key characteristics, e.g. prognostic risk, and matching these subgroups to early treatment options. The STarT-MSK programme developed and tested a new stratified primary care intervention for patients with common musculoskeletal (MSK) conditions in general practice. Stratified care involves changing General Practitioners’ (GPs) behaviour, away from the current ‘stepped’ care approach to identifying early treatment options matched to patients’ risk of persistent pain. Changing healthcare practice is challenging, and to aid the successful delivery of stratified care, education and support for GPs was required from the initial stages of the programme. This paper details the steps in integrating the development of a clinician support package throughout the 6-year programme, to support GP engagement in delivering the STarT MSK intervention. Practical recommendations are made for future general practice interventions.
Methods
Clinician support was developed through an iterative, mixed methods approach. Qualitative research with patients and GPs identified barriers and facilitators to the adoption of stratified care, which were mapped onto the Theoretical Domains Framework (TDF) and Behaviour Change Technique (BCT) taxonomy. Identified domains/BCTs were ‘translated’ into an educational paradigm, and an initial version of the support package developed. This was further refined following a feasibility and pilot RCT, and a finalised support package was developed for the main RCT.
Results
The clinician support package comprised face-to-face sessions combining adult-learning principles with behaviour change theory in a multimethod approach, which included group discussion, simulated consultations, patient vignettes and model consultation videos. Structured support for GPs was crucial in enabling fidelity and, ultimately, a successful trial. Results highlighted that clinician support is a two-way process– the study team can learn from and adapt to specific local factors and issues not previously identified. The support from senior clinicians was also required to ensure ‘buy in’, and results indicated the importance of monitoring GP performance and providing regular feedback.
Conclusion
Designing effective clinician support from the onset of trial intervention design, in an evidence-based, theory-informed manner, is crucial to encourage active engagement and intervention fidelity within the trial, enabling the delivery of a robust and reliable proof of principle trial.
Synthesis, Structure, and Properties of a Dinuclear Cu(II) Coordination Polymer Based on Quinoxaline and 3,3-Thiodipropionic Acid Ligands
Authors: Tella Adedibu Clement , Owalude Samson , Adimula Vincent , Oladipo Adetola , Olayemi Victoria , Ismail Bushra , Mumtaz Amara , Rehman Attiq , Khan Asad , Clayton Hadley , Tahir Nawaz 
Published: February 2021

Abstract
The coordination polymer [Cu2(TDPH)4(QNX)].DMF, (QNX = Quinoxaline; TDPH = 3,3-thiodipropionic acid), has been prepared by reaction of copper acetate, TDPH, and quinoxaline. The compound was characterized by elemental analysis, FTIR spectroscopy, and single-crystal X-ray diffraction. The crystal is monoclinic with a P21/n space group and dimensions of a = 12.889(3) Å, b = 14.983(4) Å, c = 14.091(3) Å, α = 90 °, β = 90.200(11) °, γ = 90 °, V = 2721.18 (2) Å3, Z = 4. The ligands are hexagonally coordinated to the Cu(II) centre in the form of Cu2O4N with one nitrogen atom from the quinoxaline ligand, and four oxygen atoms from four TDPH molecules in a monodentate fashion. The Cu-Cu bond length was 2.642(1) and 2.629(1) Å for the Cu1----Cu1 and Cu2----Cu2 bonds. The QNX ligand bridged the two copper atoms. The catalytic reduction of 4-nitrophenol to 4-aminophenol using NaBH4 in the presence of [Cu2(TDPH)4(QNX)].DMF, as catalyst was completed within 11 minutes. The 4-aminophenol product was confirmed using 1H NMR spectroscopy.
Antibiotic Resistance Pattern of Bacteroides Fragilis Isolated From Clinical and Gastrointestinal Specimens
Authors: Jasemi Seyedesomaye , Emaneini Mohammad , Ahmadinejad Zahra , Fazeli Mohammad Sadegh , Sechi Leonardo A. , Heravi Fatemah Sadeghpour , Feizabadi Mohammad Mehdi 
Published: February 2021

Abstract
Background: Bacteroides fragilis is a part of the normal gastrointestinal flora and the most prevalent anaerobic bacteria causes’ infection. It is highly resistant to antibiotics and contains abundant antibiotic resistance mechanisms. Methods: The antibiotic resistance pattern of 78 isolates of B. fragilis (56 strains from the gastrointestinal [GI] tract and 22 strains from clinical samples) was investigated using agar dilution method. The gene encoding Bacteroides fargilis toxin bft, and antibiotic resistance genes were targeted by PCR assay. Results: The highest rate of resistance was observed for penicillin G (100%) followed by tetracycline (74.4%), clindamycin (41%) and cefoxitin (38.5%). Only a single isolate showed resistance to imipenem which contained cfiA and IS1186 genes. All isolates were susceptible to metronidazole. Accordingly, tetQ (87.2%), cepA (73.1%) and ermF (64.1%) were the most abundant antibiotic-resistant genes identified in this study. MIC values for penicillin, cefoxitin and clindamycin were significantly different among isolates with the cepA, cfxA and ermF in compare with those lacking such genes. In addition, 22.7% and 17.8% of clinical and GI tract isolates had the bft gene, respectively. Conclusions: Therefore, it is of utmost importance to determine the antibiotic resistance patterns of B. fragilis periodically in different geographical areas to provide a suitable treatment profile for patients and to prevent improper antibiotic prescriptions.
Maternal Karyogene and Cytoplasmic Genotype Affect the Induction Efficiency of Doubled Haploid Inducer in Brassica napus
Authors: Zhang Wei , Ma YongTing , Zhu ZhenDong , Huang LiangJun , Ali Asif , Luo Xuan , Zhou Ying , Li Yun , Xu Peizhou , Yang Jin , Li Zhuang , Shi HaoRan , Wang JiSheng , Gong WanZhuo , Zou Qiong , Tao LanRong , Kang ZeMing , Tang Rong , Zhao ZhangJie , Li Zhi , Guo ShiXing , Fu Shaohong 
Published: February 2021

Abstract
Background

Production of pure lines by double haploid induction provides a new way to achieve homozygosity earlier in Brassica napus. Previously, it is not known, whether the induction has obvious maternal genotypic differences.
Results

In this study, different karyogene and cytoplasmic genotype of Brassica napus was pollinated with the inducers Y3380 and Y3560. Using the identification of the ploidy, fertility and SSR analysis of the induced F1 generation, it was found that the ploidy and phenotype of the induced F1 plants were consistent with maternal parent. The SNP chip analysis revealed that induction efficiency was affected by the karyogene when the maternal cytoplasmic genotype was the same. However, this efficiency was affected by the cytoplasmic genotype when the karyogenes were same, and the offspring of the ogura cytoplasm easier to high-frequency inducer gene hybridization or low-frequency infiltration.
Conclusion

The induction effect is influenced by the interaction between maternal karyogene and cytoplasmic genotype, and the results from the partial hybridization of progeny chromosomes indicate that the induction process may be attributed to the selective elimination of paternal chromosome. This study provides a basis for exploring the mechanism of DH inducer in Brassica napus, and provides new insights for utilization of inducers inbreeding.
Biomarkers predictive of long-term fertility found in vaginal lipidome of gilts at weaning
Authors: Mills Kayla M , Ferreria Christina R , Stevens Jebadiah , Stewart Kara R , Casey Theresa M 
Published: February 2021

Abstract
Background

A marker indicative of fertility potential of replacement gilts early in development would decrease culling rates in the sow herd, improve sow herd reproductive efficiency, and reduce production costs. The objective of this study was to determine if vaginal lipid profiles at 21 d postnatal (PN) could predict sow reproductive performance.
Methods

Vaginal swabs of the anterior vagina were taken at 21 ± 4 d PN from gilts born on a commercial sow production facility for lipidomic analysis. Animals were followed prospectively for two years and assigned to reproductive performance categories based on observation of estrus or piglets weaned per sow per year (PSY) across two farrowings.
Results

Lipids were extracted from cellular material collected with swabs taken from high fertility (HF; n = 28; ≥26 PSY) and infertile (IF; n = 34; no estrus, no pregnancy) animals and multiple reaction monitoring (MRM) profiling was used for lipidome analysis. Relative abundance of arachidonic acid (ARA, C20:4) and docosahexaenoic acid (DHA, C22:6) were lower (P < 0.05) in IF gilts than HF gilts, whereas abundance of the free fatty acids cerotic (C26:0), ximenic (C26:1), and nonadecanoic (C19:0) acids were greater (P < 0.05) in IF gilts. Additionally, eicosapentaenoic acid (C20:5) a precursor of prostaglandins was also higher (P < 0.05) in IF gilts.
Conclusions

The perspective of having a panel of lipids captured with vaginal swabs at weaning that can predict reproductive efficiency of gilts shows promise and warrants future research in this area.
Surveillance Plan of Lipophilic Marine Biotoxins in Molluscs from the Croatian Coast
Authors: Bogdanovic Tanja , Giacinto Federica Di , Berti Miriam , Visciano Pierina , Petričević Sandra , Rogošić Mario , Rogić Zoran , Mascilongo Giuseppina , Renzo Ludovica Di , Ferri Nicola , Schirone Maria , Listes Eddy 
Published: February 2021

Abstract
In the present study, a 4-year surveillance plan for the detection of lipophilic marine biotoxins in Mytilus galloprovincialis and Callista chione from different aquaculture farms and natural beds along the Central Adriatic coasts of Croatia was reported. The samples were analyzed by a validated LC-MS/MS method in accordance with the Regulation (EU) No 2019/627. Lipophilic marine biotoxins belonging to the okadaic acid group, except for azaspiracids, as well as yessotoxins were found at different concentrations, always below the maximum limits established by the Regulation (EC) No 853/2004. The seasonal distribution showed the highest values in summer months, and an interannual variability was also observed, probably due to environmental conditions aging on the density of harmful algal blooms. The comprehensive data reported in this study should help the future research in making advancing prediction models along the Eastern Adriatic coast.
Allometric Equation for Aboveground Biomass Estimation for Selected Trees Shrubs in Gesha - Sayilem Moist Afromontane Forest.
Authors: Merti Admassu Addi , Soromessa Teshome soromessa , Kefle Tura Bareke 
Published: February 2021

Abstract
Background: Allometric equations which are regressions linking the biomass to some independent variables that are used to estimate tree components from the forest. The generic equation developed by many authors may not adequately reveal the tree biomass in a specific region in tropics including in Ethiopia. Therefore, the use of species specific allometric equations is important to achieve higher levels of accuracy because trees of different species may differ in size and biomass. The objective of the study was to develop species-specific allometric equations for Apodytes dimidiata, Ilex mitis, Sapium ellipticum and shrubs (Galiniera saxifraga and Vernonia auriculifera) for estimating the aboveground biomass (AGB). Non-destructive sampling method was used for the measurement of tree biomass, accordingly the trees and shrubs whose Diameter at Breast Height (DBH) is ≥ 5 cm were sampled. For trees serial measurements of the height and diameter of trunk were done at 2 m intervals. For the determination of biomass of shrubs destructively sampled. Four branches were trimmed from tree and the trimmed branches were separated into leaves and wood and oven dried at 1050C and recorded to estimate the biomass of untrimmed small branches. Nested model was used and the best fit model was selected based on higher Adjusted R2, lower residual standard error and Akaike information criterion. Results: All the necessary biomass calculations were done, and biomass equations were developed for each species. The regression equations relate AGB with DBH, height (H), and density (ρ) were computed and the models were tested for accuracy based on observed data. The best model was selected based higher adj R2 and lower residual standard error and Akaike information criterion than rejected models. The relations for all selected models are significant (p<0.000), which showed strong correlation AGB with selected dendrometric variables. Accordingly, the AGB was strongly correlated with DBH and was not signiﬁcantly correlated with wood density and height individually in Ilex mitis. In combination, AGB was strongly correlated with DBH, height and wood density; are better for carbon assessment than general equations.Conclusions: The specific allometeric equation developed for the Gesha-Sayilem Afromontane Forest which can be used in similar moist forests in Ethiopia for the implementation of Reduced Emission from Deforestation and Degradation (REDD+) activities to benefit the local communities from carbon trade.
Study on a 3D-Bioprinted Tissue Model of Self-Assembled Nanopeptide Hydrogels Combined with Adipose-derived Mesenchymal Stem Cells
Authors: Zhou Guanzhou , Tian Ailing , Fan Lufeng , Shao Wenchong , Wu Han , Sun Nianfeng 
Published: February 2021

Abstract
Objective

This study aimed to observe the cell growth status and multidirectional differentiation ability in a 3D-bioprinted tissue model of self-assembled nanopeptides and human adipose-derived mesenchymal stem cells (Ad-MSCs).

Methods

Primary Ad-MSCs were isolated, cultured, and identified by flow cytometry. Tissue models were printed via 3D bioprinting technology using a "biological ink" consisting of a mixed solution of self-assembled nanopeptides and Ad-MSCs. Ad-MSCs were induced into osteogenic, adipogenic, and endothelial differentiation and compared with the control groups by staining.

Results

The nanopeptide fiber was 10-30 nm in diameter and 200-500 nm in length under the atomic-force microscope. It had the characteristics of nano-scale materials. Flow cytometry showed that the isolated and cultured cells were positive for CD29 (98.51%), CD90 (97.87%), and CD166 (98.32%) but did not express CD31 (1.58%), CD34 (2.42%), CD45 (2.95%), or human leukocyte antigen (HLA)-DR (0.53%), consistent with the immunophenotype of Ad-MSCs. Then, a tissue model was printed using the biological ink, followed by induction of differentiation of Ad-MSCs within the tissue model. Alizarin red S staining showed the formation of calcium nodules in the osteogenesis induction experimental group, and oil red O stained lipid droplets in Ad-MSCs in the adipogenesis induction experimental group, whereas the two control groups were not stained.

Conclusion

Ad-MSCs from primary cultures have the characteristics of stem cells. Self-assembled nanopeptide hydrogel is a good tissue engineering material that can serve as an extracellular matrix. Ad-MSCs in the 3D-printed tissue model using a biological ink consisting of a mixed solution of self-assembled nanopeptides and Ad-MSCs grew well and still had strong differentiation ability.
A multicenter clinical study: personalized medication for advanced gastrointestinal carcinomas with the guidance of Patient-Derived Tumor Xenograft (PDTX)
Authors: Cheng Yuan , Qin Shu-kui , Li Jin , Dai Guang-hai , Shen Bai-yong , Shen Jie-er , Ba Yi , Wang Xin-bo , Xu Ye , Zhou Lin , Ding Ke-feng , Qin Yan-ru , Yang Shu-jun , Zhu Yan-ping 
Published: February 2021

Abstract
Background: Establish patient-derived tumor xenograft (PDTX) from advanced GICs and assess the clinical value and applicability of PDTX for the treatment of advanced GICs. Method: Patients with advanced GICs were enrolled in a registered multi-center clinical study (ChiCTR-OOC-17012731). The performance of PDTX were evaluated includes: analyzing factors that affect the engraftment rate, comparing the histological consistency between primary tumors and tumorgrafts, examining the concordance between the drug effectiveness in PDTXs and clinical responses, and Identifying genetic variants and other factors associated with prognosis. Results: Thirty-three patients were enrolled in the study with the engraftment rate of 75.8% (25/33). The successfulness of engraftment was independent of age, cancer types, pathological stages of tumors, and particularly sampling methods. Tumorgrafts kept same histopathological characteristics as primary tumors. Forty-nine regimens involving twenty-eight drugs were tested in seventeen tumorgrafts. The median time for drug testing was 134.5 day. The follow up information of 10 regimens from 9 patients were obtained. The concordance of drug effectiveness in PDTXs and clinical responses was 100%. The tumor mutation burden (TMB) was correlated with the effectiveness of single drug regimens, while the outgrowth time of tumorgrafts was associated with the effectiveness of combined regimens. Conclusion: The engraftment rate in advanced GICs is higher than other cancers and meets the general acceptable standard of applying personalized therapeutic strategies. Tumorgrafts from PDTX kept attributes of primary tumor. Predictions from PDTX modeling highly agree with the clinical drug responses. PDTX may already be clinical applicable for the personalized medication in advanced GICs.
Ultrasonic synthesized Konjac gum/PEG-silver nanoparticles for colorimetric detection of hydrogen peroxide
Authors: KARAKUŞ SELCAN , Taşaltın Nevin , Taşaltın Cihat , Üllen Nuray Bekoz 
Published: February 2021

Abstract
Green and low-cost synthesis strategy for ultrasonic preparation of polymer blend matrix based silver nanoparticles (Ag NPs) and the development of rapid and high sensitive detection route have a great attention in biomedical applications. Therefore, in this study, we investigated the hydrogen peroxide detection performance of Konjac gum (KG)/PEG-Ag NPs. The KG/PEG-Ag NPs was synthesized via an ultrasonic process and characterized by different techniques such as ultraviolet–visible spectroscopy (UV–Vis), Fourier-Transform Infrared spectroscopy (FT-IR), Scanning Electron Microscope (SEM) and Energy Dispersive X-ray spectroscopy (EDX). Furthermore, we determined the experimental optimization on the effect of the rheological parameters of nanostructure with the highest correlation constant (R 2 : 0.989-0.996), and the intrinsic viscosity (14.71-26.77 dl/g). To provide the miscible polymer blends and homogeneous dispersion of the nanostructure, we compared the rheological parameters with the experimental results. The response time was less than 5 s and the lower limit of detection was 0.071 μM. This novel highly sensitive, rapid, and naked-eye colorimetric biosensor based Ag NPs which are prepared ultrasonic manufacturing approach, opens up a green approach of development facile and rapid detection of hydrogen peroxide in practical biomedical applications.