bluuebunny/crossref_metadata_2025_split huggingface dataset:
Shaping and properties of thermoplastic scaffolds in tissue regeneration: The effect of thermal history on polymer crystallization, surface characteristics and cell fate
Authors: Calore Andrea Roberto , Srinivas Varun , Anand Shivesh , Albillos-Sanchez Ane , Looijmans Stan F. S. P. , van Breemen Lambert C. A. , Mota Carlos , Bernaerts Katrien , Harings Jules A. W. , Moroni Lorenzo 
Published: October 2021

Abstract
Thermoplastic semi-crystalline polymers are excellent candidates for tissue engineering scaffolds thanks to facile processing and tunable properties, employed in melt-based additive manufacturing. Control of crystallization and ultimate crystallinity during processing affect properties like surface stiffness and roughness. These in turn influence cell attachment, proliferation and differentiation. Surface stiffness and roughness are intertwined via crystallinity, but never studied independently. The targeted stiffness range is besides difficult to realize for a single thermoplastic. Via correlation of thermal history, crystallization and ultimate crystallinity of vitamin E plasticized poly(lactide), surface stiffness and roughness are decoupled, disclosing a range of surface mechanics of biological interest. In osteogenic environment, human mesenchymal stromal cells were more responsive to surface roughness than to surface stiffness. Cells were particularly influenced by overall crystal size distribution, not by average roughness. Absence of mold-imposed boundary constrains makes additive manufacturing ideal to spatially control crystallization and henceforward surface roughness of semi-crystalline thermoplastics.

Graphic abstract
A Mini-Open Approach to Medial Pinning in Pediatric Supracondylar Humeral Fractures May Be Safer Than Previously Thought
Authors: Rees Andrew B. , Schultz Jacob D. , Wollenman Lucas C. , Moore-Lotridge Stephanie N. , Martus Jeffrey E. , Schoenecker Jonathan G. , Mencio Gregory A. 
Published: October 2021

Background:
Displaced pediatric supracondylar humeral fractures (SCHFs) are stabilized after reduction by smooth pins. Although some SCHFs are biomechanically stable after lateral-only entry pinning (lateral pinning), an additional medial entry pin (cross-pinning) confers superior stabilization in some SCHFs. There is a recognized risk of iatrogenic ulnar nerve injury with medial entry pinning. The best existing evidence has estimated an iatrogenic ulnar nerve injury rate of approximately 3.4% in cross-pinning. In similar studies, the rate of iatrogenic nerve injury (all nerves) in lateral pinning is estimated at 1.9%. This study aimed to use a large, single-center, single-technique (mini-open) retrospective case series to determine the rate of iatrogenic ulnar nerve injury in cross-pinning.

Methods:
Patients undergoing percutaneous cross-pinning via the mini-open technique for SCHFs from 2007 to 2017 were retrospectively reviewed. Injury characteristics, operative variables, fixation technique, and complications, such as iatrogenic nerve injury, were recorded. Patients who underwent operative treatment at another hospital, had no postoperative follow-up, or died due to polytrauma were excluded.

Results:
In this study, 698 patients undergoing cross-pinning during the study period were identified. Patients treated with cross-pinning had severe fractures, including a total of 198 preoperative neurovascular injuries (28.4%), 32 patients (4.6%) with skin tenting, and 19 patients (2.7%) with open fractures. Iatrogenic nerve injury was reported in 3 cases (0.43%), all of which affected the ulnar nerve. In 2 of 3 cases of iatrogenic nerve injury, the ulnar nerve symptoms resolved at a mean follow-up of 15 weeks.

Conclusions:
The mini-open approach for medial pin insertion is safer than previous estimates. Here, in the largest single-center study of cross-pinning for SCHFs, the iatrogenic ulnar nerve injury rate of 0.43% was nearly 10 times lower than estimated rates from recent meta-analyses. Considering all nerves, the iatrogenic injury rate for this cross-pinning cohort was also lower than the estimated iatrogenic nerve injury rate for lateral pinning.

Level of Evidence:
Therapeutic Level IV. See Instructions for Authors for a complete description of levels of evidence.
An integrated assessment of seismic hazard exposure and its societal impact in Seven Sister States of North Eastern Region of India for sustainable disaster mitigation planning
Authors: Agrawal Navdeep , Gupta Laxmi , Dixit Jagabandhu , Dash Sujit Kumar 
Published: October 2021

Abstract
The Seven Sister States of the North Eastern Region of India, located on the complex seismotectonic belt, is characterized by high seismicity. A comprehensive seismic hazard exposure assessment is carried out by quantifying hazard using a probabilistic approach, vulnerability by factor analysis, and exposure mapping by integrating seismic hazard and vulnerability. Peak ground acceleration (PGA) values at bedrock are calculated with the help of ground motion prediction equations (GMPE) for 10% probability of exceedance in 50 years (475 years) and 100 years (950 years), and 2% probability of exceedance in 50 years (2475 years). The resulting spatial distribution of the PGA values considering return periods of 475, 950, and 2475 years are presented through seismic hazard maps. The social vulnerability analysis indicates that 21 districts covering 91.43% area of the state of Assam and the entire state of Tripura are under high vulnerability. With the help of spatial cluster analysis, it is found that 17.14% of the study area are having an average social vulnerability index (SVI) score of 0.329 and therefore can be considered as hotspots. Through seismic hazard analysis, it is observed that more than 50% of the area of North East India is under moderate to very high exposure class. The seismic hazard maps developed can help in disaster mitigation planning and execution leading to sustainable development goals and targets.
Effects of High-Intensity Interval Training on Improving Arterial Stiffness in Chinese Normal Weight Obese Female University Students: A Pilot Randomized Controlled Trial
Authors: Hu Jingyun , Zhu Lei , Yang Ruoyu , Wang Liyan , Liang Leichao , Yang Yuanyuan , Jia Shihao , Chen Ruiyi , Liu Qianle , Ren Yu , Cai Ming 
Published: October 2021

Abstract
Background: High intensity interval training (HIIT) has been reported to exert better effects on cardiovascular fitness in obesity, but little known about the arterial stiffness (AS) in normal weight obesity (NWO) females. Thus, this study investigated the changes of body composition, heart rate (HR), blood pressure (BP), blood lipids as well as the parameters of propensity for AS (arterial velocity pulse index (AVI) and arterial pressure volume index (API)).Methods: Forty NWO female university students were randomly assigned to the control group (n=20) or the HIIT group (30 minutes at 90% HRmax, n=20). The participants in HIIT group accepted a four-week HIIT intervention for five days a week. The above parameters of body composition, cardiometabolic biomarkers, lipid profile, and AS were assessed before and after the HIIT intervention. Results: HIIT significantly improved the body composition in decreasing the BMI (p=0.001), BF% (p<0.001), BFM (p<0.001), LBFM (p<0.001), LMC (p=0.016), and Obesity degree (p=0.001), and increased the SMM (p=0.020), Protein content (p=0.035), TBW (p=0.017), FFM (p=0.019), BCM (p=0.020), and Inbody score (p<0.001). HIIT also affected the cardiometabolic biomarkers by decreasing the HR (p=0.001), SBP (p<0.001), and DBP (p<0.001). As for the lipid profile, HIIT obviously ameliorated the blood lipids metabolism by decreasing the levels of TC (p=0.001), TG (p<0.001), LDL (p<0.001), and TC/HDL (p<0.001), and increasing the levels of HDL (p<0.001). In addition, the AS was improved by HIIT intervention through decreasing the AVI (p<0.001) and API (p<0.001).Conclusions: Our results provided herein demonstrate that this pattern of a 4-week HIIT had a positive impact on physical fitness in the aspect of body composition, lipid profile, and cardiovascular function in NWO female university students.Trial registration: ChiCTR2100050711. Registered 3 September 2021. Retrospectively registered.
Transcriptomic Analysis of Stropharia Rugosoannulata Reveals Potential Carbohydrate Metabolism and Cold Resistance Mechanisms Under Low-Temperature Stress
Authors: Hao Haibo , Zhang Jinjing , Wu Shengdong , Bai Jing , Zhuo Xinyi , Zhang Jiaxin , Kuai Benke , Chen Hui 
Published: October 2021

Abstract
Low temperature is an important environmental factor that restricts the growth of Stropharia rugosoannulata; however, the molecular mechanisms underlying S. rugosoannulata responses to low-temperature stress are largely unknown. In this study, we performed a transcriptome analysis of a high-sensitivity strain (DQ-1) and low-sensitivity strain (DQ-3) under low-temperature stress. The liquid hyphae of S. rugosoannulata treated at 25°C and 10°C were analyzed by RNA-Seq, and a total of 9499 differentially expressed genes (DEGs) were identified. GO and KEGG enrichment analyses showed that these genes were enriched in “xenobiotic biodegradation and metabolism”, “carbohydrate metabolism”, “lipid metabolism” and “oxidoreductase activity”. Further research found that carbohydrate enzyme (AA, GH, CE, and GT) genes were downregulated more significantly in DQ-1 than DQ-3 and several cellulase activities were also reduced to a greater extent. Moreover, the CAT1, CAT2, GR, and POD genes and more heat shock protein genes (HSP20, HSP78 and sHSP) were upregulated in the two strains after low-temperature stress, and the GPX gene and more heat shock protein genes were upregulated in DQ-3. In addition, the enzyme activity and qRT–PCR results showed trends similar to those of the RNA-Seq results. This result indicates that low-temperature stress reduces the expression of different AA, GH, CE, and GT enzyme genes and reduces the secretion of cellulase, thereby reducing the carbohydrate metabolism process and mycelial growth of S. rugosoannulata. Moreover, the expression levels of different types of antioxidant enzymes and heat shock proteins are also crucial for S. rugosoannulata to resist low-temperature stress. In short, this study will provide a basis for further research on important signaling pathways, gene functions and variety breeding of S. rugosoannulata related to low-temperature stress.
A Radiomic Model To Classify Response To Neoadjuvant Chemotherapy in Breast Cancer
Authors: McAnena Peter , Moloney Brian , Browne Robert , O’Halloran Niamh , Walsh Leon , Walsh Sinead , Sheppard Declan , Sweeney Karl , Kerin Michael , Lowery Aoife 
Published: October 2021

Abstract
Background Medical image analysis has evolved to facilitate the development of methods for high-throughput extraction of quantitative features that can potentially contribute to the diagnostic and treatment paradigm of cancer. There is a need for further improvement in the accuracy of predictive markers of response to neo-adjuvant chemotherapy (NAC). The aim of this study was to develop a radiomic classifier to enhance current approaches to predicting the response to NAC breast cancer. Methods Data on patients treated for breast cancer with NAC prior to surgery who had a pre-NAC dynamic contrast enhanced (DCE) breast MRI were included. Response to NAC was assessed using the Miller-Payne system on the excised tumour. Tumour segmentation was carried out manually under the supervision of a consultant breast radiologist. Features were selected using least absolute shrinkage selection operator (LASSO) regression. A support vector machine (SVM) learning model was used to classify response to NAC. Results 74 patients were included. Patients were classified as having a poor response to NAC (reduction in cellularity <90%, n=44) and an excellent response (>90% reduction in cellularity, n=30). 4 radiomics features (discretized kurtosis, NGDLM contrast, GLZLM_SZE and GLZLM_ZP) were identified as pertinent predictors of response to NAC. A SVM model using these features stratified patients into poor and excellent response groups producing an AUC of 0.75. Addition of estrogen receptor (ER) status improved the accuracy of the model with an AUC of 0.811. Conclusion This study identified a radiomic classifier incorporating 4 radiomics features to augment subtype based classification of response to NAC in breast cancer.
Diversity and health risk potentials of the Enterococcus population in tropical coastal water impacted by Hurricane Lane
Authors: Saingam Prakit , Di Doris Y. W. , Yan Tao 
Published: October 2021

Abstract
Hurricane-caused stormwater runoffs transport diverse terrestrial pollutants, adversely impact microbiological water quality, and introduce fecal and other pathogens to coastal water environments. This study investigated the genotypic diversity, phylogenetic composition, antibiotic resistance patterns, and virulence gene repertoire of the Enterococcus population in the Hilo Bay coastal water after the immediate impact of Hurricane Lane. DNA fingerprinting of Enterococcus isolates exhibited large genotypic diversity, while 16S rRNA gene sequencing identified four major species, including E. faecalis (34.7%), E. faecium (22.4%), E. hirae (22.4%), and E. durans (18.4%). Four common enterococcal virulence genes (cylA, esp, asa1, and gelE) were detected in the Enterococcus population, with significant portions of E. durans (33.3%), E. faecalis (41.2%), E. faecium (36.4%), and E. hirae (27.3%) isolates possessing two or more virulence genes. Considerable antibiotic resistance to rifampin, erythromycin, tetracycline, and nitrofurantoin was detected in the Enterococcus population, with one E. durans isolate showing vancomycin resistance. The results indicate considerable health implications associated with Enterococcus spp. in the hurricane-impacted tropical coastal water, illustrating the needs for more comprehensive understanding of the microbiological risks associated with storm-impacted coastal water.
Analysis on the effect of large-scale compound ecological engineering system on pollution control of the estuary of a lake
Authors: Jiang Yongbo , Xu Xiaoyi , Wu Wei , Wang Bin , Sun Yidong , Chen Xiaobin , Shen Zhengdong , Huang Tianyin 
Published: October 2021

Abstract
The quality of the water and the water environment in the estuary of a lake directly affect the water quality and ecological functions of the entire lake. Multi-technology systems, which integrate biotechnological analysis of a lake estuary and restoration of the ecological functions of the water in situ, have gradually been adopted for lake management and restoration. The Xielugang Estuary is located north of the Yangcheng Middle Lake and always exhibits a certain degree of eutrophic phenomena. To ensure the safety of the ecological environment in Yangcheng Lake, a multi-level purification and ecological system with ‘intercept precipitation–ecological restoration–coupled biological treatment’ was developed. Water quality monitoring results for the inlet and outlet of different units in the system from October 2020 to May 2021 showed that the system was effective. We also found that the purification capacity of the composite system was high and the system could significantly enhance the reduction of total nitrogen, total phosphorus, potassium permanganate index and total suspended solids. The average removal rates for these components were 41.34, 61.76, 35.21 and 67.21%, respectively, and the removal rate for typical heavy metals (Zn, Cu, Cd, and Pb) was 30.4–48.9%. The composite system substantially improved the water quality of the estuary and the wetland ecological function, demonstrating its effectiveness and significance.
Enhancing chlorine resistance in polyamide membranes with surface & structure modification strategies
Authors: Idrees Muhammad Faisal , Tariq Umar 
Published: October 2021

Abstract
Higher efficient reverse osmosis (RO) membrane development is a significant issue due to the payoff among salt rejection and water flux and permissive chlorine attacking and fouling potential. Weak chlorine resistance is a distinctive challenge for composite polyamide thin-film reverse osmosis membranes. A commercial aromatic membrane was modified by grafting nitrogen-doped graphene oxide quantum dots (N-GOQDs) to enhance chlorine resistance, embedding two-dimensional MXene Ti3C2Tx, introducing synthetically reductive thioether units and oxidized graphitic carbon nitride (OGCN). In this work, salt rejection, chlorine resistance, and water flux increased compared to the pristine membrane. Comprehensive arrangement of desalination performance and chlorine resistance was achieved by varying time and concentrations of prepared chemicals. For instance, improved chlorine resistance, after 12 hours of grafting time by N-GOQDs doped membrane, was 32.8%, after 6 hours of exposure time by MXene Ti3C2Tx membrane was 27.4%, after 1 hour of exposure time by thioether membrane was 28.1% and after 40 hours of doping time by OGCN membrane was 31.3%. N-GOQDs doped membrane showed a good chlorine resistant property, but on the other hand, thioether nano units showed other properties more effectively, including water flux, salt rejection, and less reaction time.
Simultaneous removal of fluoride, manganese and iron by manganese oxide supported activated alumina: characterization and optimization via response surface methodology
Authors: You Kun , Gao Yujia , Qian Weiyi , Fu Jinxiang , Wang Juliang , Zhou Weiwei 
Published: October 2021

Abstract
Fluoride, iron and manganese simultaneous exceedance of standard can be observed in groundwater in northeastern China. This work aims to apply a highly efficient method combining adsorption and oxidation for the synchronous removal of the inorganic ions. An innovative adsorbent (manganese-supported activated alumina) was synthesized by the impregnation method and showed a significant adsorption capacity better than that of fresh activated alumina. The characterization (scanning electron microscope; Brunauer, Emmett and Teller; X-ray diffraction and Fourier transform infrared spectroscopy) results verified the successful introduction of MnOOH and MnO2, and the improvement of surface microstructure enhanced the removal ability. The effect of single factors, such as pH value, reaction time or dosage on the removal performance has been verified. The maximum removal efficiencies of fluoride, iron and manganese were optimized via Response surface methodology considering the independent factors in the range of MO@AA dosage (5–9 g/L), pH (4–6) and contact time (4–12 h). Noted that compared with control, MO@AA exhibited 59.4% of improved fluoride performance. At pH of 5.79, contacting time of 12 h and 8.21 g/L of MO@AA, fluoride, iron and manganese removal were found to be 91, 100 and 23%, respectively. Herein, MO@AA was distinguished as good applicability for the treatment of fluoride-, iron- and manganese-containing groundwater.
The Effect of the Frequency and Source of Instructional Messages on Knowledge Sharing Behavior
Authors: Cheng Xu (Joyce) 
Published: October 2021

ABSTRACT
Prior literature has documented the importance of knowledge sharing to accounting and auditing; however, researchers have made little progress in understanding knowledge sharing behavior in an accounting setting. This study aims to understand how formal communication among auditors affects knowledge sharing behavior. Specifically, this study investigates the impact of the frequency and source of instructional messages on knowledge sharing behavior in an auditing setting. The results of this study indicate that an in-group member is more effective in promoting knowledge sharing behavior. When motivating knowledge sharing behavior, an out-group member should avoid sending a high frequency of knowledge sharing messages. This study provides guidelines for altering communication styles in order to encourage knowledge sharing. The findings of this study also suggest that more communication messages are not always better. Thus, these findings can help organizations understand the reasons why employees are reluctant to share their knowledge.
Sodium–Glucose Cotransporter 2 Inhibitor Use Associated With Fournier’s Gangrene: A Review of Case Reports and Spontaneous Post-Marketing Cases
Authors: Tran Bao Anh , Updike Wendy H. , Bullers Krystal , Serag-Bolos Erini 
Published: October 2021

Background
Sodium–glucose cotransporter 2 (SGLT2) inhibitors are effective for glycemic control and have demonstrated cardiorenal benefits. The U.S. Food and Drug Administration (FDA) released a boxed warning in 2018 regarding the potential development of Fournier’s gangrene (FG) with the use of SGLT2 inhibitors. FG is a serious perineal infection with a mortality rate of up to 88% in some cases.

Objectives
To report spontaneous post-marketing cases from the FDA Adverse Event Reporting System (FAERS) database and case reports from the literature of FG associated with the use of SGLT2 inhibitors and to determine whether correlations exist with specific agents.

Methods
A search of the FAERS database was conducted to identify reported cases of FG associated with the use of any FDA-approved SGLT2 inhibitor between 1 March 2013 and 30 June 2020. Additionally, a literature search was conducted of PubMed, Embase, and the Cochrane library using PRISMA guidelines to identify case reports of FG with the use of SGLT2 inhibitors up to 9 October 2020.

Results
A total of 491 cases from the FAERS database were included for review. Descriptive analysis depicted more cases in the empagliflozin, canagliflozin, and dapagliflozin groups than in the ertugliflozin group. Nine case reports were included from the literature review; four attributed to dapagliflozin, three to empagliflozin, and two to canagliflozin. The median ages from cases reported in the FAERS database and from the literature review were 54 and 52 years, respectively. In both datasets, males had a higher incidence of FG than females. Additional data reported include clinical outcomes and concomitant antihyperglycemic medications.

Conclusion
Consistent findings are noted in this systematic review and warrant further investigation to elucidate the association between SGLT2 inhibitor use and the development of FG. These results may drive enhanced prescribing patterns to consider patient-specific risk factors and timely monitoring, especially as more indications are approved related to these medications’ cardiorenal protective properties.
Influence of National Competitiveness Indicators on the Export Performance of the Visegrad Group Plus Countries
Authors: Majerová Ingrid , Nevima Jan 
Published: October 2021

Abstract
A high level of competitiveness is the goal of every economic entity, whether individuals, companies, regions or nations. This paper discusses the issue of national competitiveness, which can be further divided into non-measurable and measurable input and output indicators. Output indicators are analysed in this paper, among which are a degree of openness, export performance, transformational performance and relative power of specialization. The aim is to find the relations among these indicators in the selected EU countries that form the Visegrad Group Plus, namely the Czech Republic, Slovakia, Hungary, Poland, Austria and Slovenia. These linkages have been tested by a panel regression model during the period 1995 to 2015. Strong impact of all indicators on export performance was assumed. However, this hypothesis was proved only partially, namely regarding the influence of openness degree and transformational performance on the export performance in period fixed effects.