bluuebunny/crossref_metadata_2025_split huggingface dataset:
PARP1 Bound to XRCC2 Promotes Tumor Progression in Colorectal Cancer
Authors: Xu Kaiwu , Yu Zhige , Lu Tailiang , Peng Wei , Gong Yongqiang , Chen Chaowu 
Published: February 2021

Abstract
Background: By complexing poly (ADP-ribose) (PAR) in reaction to breaked strand, PAR polymerase1 (PARP1) acts as the key enzyme participated in DNA repair. However, recent studies suggest that unrepaired DNA breaks results in persistent PARP1 activation, which leads to a progressively reduce in hexokinase1 (HK1) activity and cell death. So the molecular mechanism of PARP1 remains elusive. Methods: 212 colorectal cancer (CRC) patients who had the operation at our hospital were recruited. Used immunohistochemistry to evaluate PARP1 expression. Survival analysis was calculated based on PARP1 expression.Results: Compared with matching adjacent noncancerous tissue, in CRC tissue, PARP1 expression was remarkably higher, which was correlated with the degree of differentiation, TNM stage, depth of invasion, distant metastasis, and 5-year survival. Furthermore, after constructing CRC cell lines stably expressing low or high PARP1, we found that PARP1 overexpression promoted proliferation, and proved PARP1 interacted with XRCC2 in CRC cells by immunoprecipitation (IP) analysis.Conclusions: PARP1 was upregulated in CRC cells and promoted its proliferation of colorectal cancer cells. Furthermore, PARP1 expression status was significantly related to some clinicopathological features and 5-year survival.
OPG / RANKL / RANK Gene Methylation Among Alcohol-induced Femoral Head Necrosis in Northern Chinese Men
Authors: Wang Tiantian , Wang Fei , Liu Tingting , Sun Menghu , An Feimeng , Liu Chang , Tian Ye , Cao Yuju , Wang Jianzhong 
Published: February 2021

Abstract
Background and purpose: Alcohol-induced osteonecrosis of the femoral head (ONFH) is a complex and heterogeneous disease. Genetic factors and epigenetic modifications are one of the pathogenesis of the disease. However, the influence of epigenetic factors on the disease has not been systematically studied. Our research aims to determine the methylation changes of alcohol-induced ONFH.Methods：An analytical cross-sectional study of a Chinese male population (50 lung cancer patients and 50 controls). The EpiTYPER of the Sequenom MassARRAY platform was used to detect the DNA methylation status of 132 Cytosine-phosphate-Guanine (CpG) sites in the OPG/RANKL/RANK gene promoter region.Results: In the whole study group，Chi-square test was used to analyze the methylation rate between the two groups, and six CpG sites were found to be different, among which OPG1_CpG_2, OPG3_CpG_4, RANK1_CpG_6, RANK3_CpG_10, RANKL2_CpG_21, and RANKL2_CpG_46 in the case group were higher than those in the control group, while OPG4_CpG_2 was lower than that in the control group. Our results showed that 146 CpG sites were measured, of which 32 were undetectable, and of the remaining 114 methylation sites, methylation levels were different in 23 CpG sites in patients with alcohol-induced ONFH compared to healthy controls. Receiver operator characteristic (ROC) curve analysis demonstrated the methylation levels of OPG/RANKL/RANK could efficiently predict the existence of alcohol-induced ONFH.Conclusion: Our study of Chinese men suggests that several CpG sites in the OPG/RANKL/RANK gene in peripheral blood leukocytes of patients with alcohol-induced ONFH are in abnormal methylation state(hypermethylation tended to be more frequent).
The influence of entrepreneurial passion in the relationship between creativity and entrepreneurial intention
Authors: Murad Majid , Li Cai , Munir Muhammad , Ashraf Sheikh Farhan , Arora Surhbi 
Published: February 2021

Abstract
This article aims to identify the impact of creativity on entrepreneurial intention with the mediating role of entrepreneurial passion. This study applied the SEM-structural equation modeling technique to test the hypotheses on a sample of 390 university students from Pakistan. The findings of the study revealed that creativity positively and significantly influenced entrepreneurial intention. The results also indicated that entrepreneurial passion partially mediates in the relationship between creativity and entrepreneurial intention. The results of the study made an innovative contribution to Pakistan’s higher education sector, researchers, and policymakers to unlock the hidden potential of its youth and further contribute to designing the relevant practical implications.
Co-creating Physical Activity Interventions: a Mixed Methods Evaluation Approach
Authors: Popp Johanna , Grüne Eva , Carl Johannes , Semrau Jana , Pfeifer Klaus 
Published: February 2021

Abstract
Background: Co-creation strategies, such as cooperative planning, are promising as a means to ensure that physical activity interventions address real-world problems and are tailored to the target group. This has already been validated in diverse settings. However, questions targeting the transferability of cooperative planning to new settings and the key factors influencing its success or failure remain unclear. At the same time, co-creation processes are complex and evaluation can be challenging. Following calls for detailed reporting, this paper describes the programme activities, the underlying logic, and methodological design of a study that aims to evaluate the transfer of cooperative planning to new settings and to explore the associated key determinants.Methods: Cooperative planning was utilised as a strategy to target physical activity promotion in three real-world German settings in the nursing care and automotive mechatronics sectors. This involved researchers working alongside stakeholders from practice and policy to conjointly develop new interventions to promote physical activity in physically demanding jobs. A pragmatic approach is used to evaluate both the transferability and key determinants of this strategy. We developed a logic model for this co-creation process that describes the underlying assumptions and guides the evaluation. The evaluation outcomes of this study include planning meetings, newly developed interventions, and the determinants that are likely to affect cooperative planning. Quantitative and qualitative data will be collected using questionnaires, documents, and interviews. The quantitative data will be analysed descriptively, while the qualitative data will mainly be analysed using qualitative content analysis, split by settings. Subsequently, data triangulation will be used to integrate the quantitative and qualitative findings, which will then be compared across all three settings.Discussion: The study findings will contribute to a better understanding of co-creation strategies, their transferability and key determinants. The practical implications can include a checklist for assessing key determinants and a guideline for transferring cooperative planning into new settings to benefit more people. Ultimately, this study will help to advance co-creation strategies and may be relevant for researchers, practitioners, and policymakers targeting physical activity promotion in various contexts.Trial registration: Open Science Framework: https://osf.io/r6xnt/ (retrospectively registered)
Bronchial Carcinoid Tumor in the Era of Covid-19 Pandemic: A Case Report
Authors: Mindaye Esubalew T. , Kassahun Mulugeta , Tigiye Gulilat 
Published: February 2021

Abstract
Introduction: Bronchial carcinoid tumors are rare, slow growing, malignant neuroendocrine tumors which arise from Enterochromaffin (Kulchitsky) cells. Early diagnosis is extremely important as the main stay of treatment is surgical excision. Presentation of case: We present a rare case of bronchial typical carcinoid tumor in a 27-year-old male who presented with a complaint of intermittent dry cough of 2 weeks’ duration associated with shortness of breath, low grade intermittent fever and loss of appetite. He was initially misdiagnosed as covid-19 pneumonia and was admitted to covid-19 treatment center. Right lung bi-lobectomy with regional lymph node resection was done and he was discharged home in good condition.Discussion: Majority of typical carcinoids are located in the central airways leading to bronchial obstruction with recurrent pneumonia, chest pain, wheezing and hemoptysis. Due to such nonspecific presentation most patients are misdiagnosed or diagnosed late. In the era of covid-19 pandemic, in addition to the wide spread anxiety on the health care workers access to diagnostic work up of patients with rare diseases like bronchial carcinoids is very challenging especially in resource limited areas as invasive diagnostic procedures like bronchoscopy are usually avoided in covid-19 suspected patients during the pandemic. Although hilar and mediastinal lymph nodes are the most common metastatic sites for typical carcinoids most lymphadenopathies are caused by a reactive inflammatory reaction.Conclusion: Bronchial carcinoids are rare, malignant neuroendocrine tumors with complete surgical resection being the only curative management. Although the world is over burdened by Covid-19 pandemic crisis, other lung pathologies are also still present in communities. In such challenging circumstance diagnosing rare pulmonary diseases like bronchial carcinoids need high index of suspicion and meticulous diagnostic work up. The outcome of typical carcinoids with lymph node metastasis is excellent with complete resection but close follow up is mandatory when dealing with larger tumors.
Sexually Dimorphic Expression and Special Regulatory Sequence of Dnali1 in the Olive Flounder Paralichthys Olivaceus
Authors: Wang Ling , Tan Xungang , Zou Congcong , Wang Lijuan , Wu Zhihao , Zou Yuxia , Song Zongcheng , You Feng 
Published: February 2021

Abstract
Dynein axonemal light intermediate chain 1 (dnali1) is an important part of axonemal dyneins and plays an important role in the growth and development of animals. However, there is little information about dnali1 in fish. Herein, we cloned dnali1 gene from the genome of olive flounder (Paralichthys olivaceus), a commercially important maricultured fish in China, Japan, and Korea, and analyzed its expression patterns in different gender fish. The DNA sequence of the entire gene contained a 771 bp open reading frame (ORF), two different sizes of 5' untranslated region (5'UTR), and a 1499 bp 3' untranslated region (3'UTR). Two duplicated 922 nt fragments were found in dnali1 mRNA. The first fragment contained the downstream coding region and the front portion of 3'UTR, and the second fragment was entirely located in 3’UTR. Multiple alignments indicated that the flounder Dnali1 protein contained the putative conserved domain. Its expression showed sexually dimorphic with predominant expression in the flounder testis, and lower expression in other tissues. The gene with the larger 5’UTR was specifically expressed in the testis. The highest expression level in the testis was detected at stages Ⅳ and Ⅴ. Transient expression analysis showed that the 922 bp repeated sequence 3’UTR of dnali1 down-regulated the expression of GFP at the early stage in zebrafish. The results implied that dnali1 might play an important role in flounder testis, especially in the period of spermatogenesis, and the 5’UTR and the repetitive sequences in 3’UTR might contain some special regulatory elements for the cilia.
Study on Mechanical Mechanism and Stability of Surrounding Rock in Fault Structure Roadway
Authors: lu yuming , Wei Wang , Zhiyu1 Tang 
Published: February 2021

Abstract
In order to study the deformation and failure mechanism of the fault passage, this paper makes a series of research on the fault passage through theoretical analysis, field investigation and numerical simulation. Firstly, the mechanical characteristics of the fault structure and the deformation and failure characteristics of the surrounding rock passing through the fault are summarized. Then, the numerical analysis is carried out before and after the tunnel passing through the fault. The results show that the original support scheme has large deformation and failure in the surrounding rock of the fault section, and the deterioration and expansion of the plastic zone leads to the failure of the support. Finally, the comprehensive support scheme and principle of "bolt + anchor cable + metal mesh + grouting" is put forward, and the support for the broken tunnel passing through the fault is strengthened. The calculation results show that the support scheme can keep the tunnel passing through the fault in a stable deformation range, which is conducive to the long-term stability of the surrounding rock.
Kimura’s Disease With Recurrent Bilateral Lacrimal Gland Involvement in a Male Japanese Child Successfully Treated With Cyclosporine A
Authors: Sugimoto Keisuke , Enya Takuji , Morimoto Yuichi , Oshima Rina , Miyazaki Kohei , Okada Mitsuru 
Published: February 2021

Abstract
BackgroundKimura’s disease (KD) is a rare chronic inflammatory disease of unknown etiology. Clinically, KD is characterized by nodular subcutaneous masses, typically localized to the neck and head. Involvement of the lacrimal glands and limbs is uncommon and seldom reported. Case presentationWe report a case of a 4-year-old Japanese boy presenting with bilateral upper eyelid swelling with nodular subcutaneous lesions and peripheral eosinophilia. Based on clinical, histopathological, and laboratory findings, the patient was diagnosed with KD. An itchy subcutaneous mass on the left arm developed at the age of 14 years. Treatment with steroids was effective, but the masses relapsed within a few months as the steroids were tapered after the patient developed side effects. Treatment with cyclosporine A was initiated, which led to the improvement of clinical features and serial levels of cytokines. ConclusionsWe report a rare case of KD with a peculiar clinical presentation. The patient responded well to treatment with CsA.
Long-term Results of Topical 0.02% Tacrolimus Ointment for Refractory Ocular Surface Inflammation in Pediatric Patients
Authors: Koh Kyungmin , Jun Ikhyun , Kim Tae-im , Kim Eung Kweon , Seo Kyoung Yul 
Published: February 2021

Abstract
Background: To investigate the efficacy and safety of long-term treatment with topical 0.02 % tacrolimus ointment for ocular inflammation in pediatric patients. No studies have been reported on the outcome of long-term (≥12 months) use of topical tacrolimus in pediatric patients for treatment of ocular surface diseases. Methods: A total of 144 eyes of 72 pediatric patients who were prescribed topical 0.02% tacrolimus ointment between January of 2010 and March of 2018 for anterior segment inflammatory disorders refractory to conventional steroid therapy were included. Patients completed questionnaires and underwent slit-lamp examinations for evaluation of symptoms and signs at baseline. Patients were followed 1, 2, 3, 6, 9 and 12 months after initiation of tacrolimus treatment. After 12 months, patients were followed every 3 months for monitoring of possible adverse events and clinical outcomes. Changes in ocular surface parameters during slit-lamp examination, clinical symptoms and concurrent steroid use were graded with a scoring system. The presence of side effects was also verified.Results: Among 72 patients (56 % males, age 10.79 ± 3.96 years), 25 patients (48% males, age 11.42 ± 3.91 years) fully recovered, resulting in discontinuance of the ointment treatment before 12 months. Six patients experienced intolerable burning sensation, which required treatment cessation. Cessation days of those who quit were 1,5,14,20,26, and 35 days. Seven patients were lost during follow-up. Thirty-four patients (56% males, age 11.20 ± 4.21 years) were treated with tacrolimus ointment for over 12 months (average 23.12 ± 19.07 months). During the follow-up period, all patients showed improved clinical signs and symptoms, and no adverse reaction was noted. Conclusions: Long-term maintenance of topical tacrolimus 0.02% ointment is safe and effective in improving refractory ocular surface inflammatory diseases in pediatric patients.
Clinical characteristics and surgical treatments of primary hepatic angiosarcoma
Authors: Jiang Lei , Xie Lijun , Li Ge , Xie Hang , Fang Zhao , Cai Xinran , Chen Yanling 
Published: February 2021

Abstract
Purpose
Primary hepatic angiosarcoma is a very rare and highly malignant tumor with poor prognosis. It is difficult to diagnose because of the lack of typical clinical features, and the treatment protocols for PHA are also not clear. Therefore, this study wants to find out the clinical characteristics and surgical treatments of primary hepatic angiosarcoma.
Methods
Among 8990 patients diagnosed with primary malignant tumor of the liver from January 2000 to December 2019 in our hospital, only four patients were diagnosed with primary hepatic angiosarcoma. The demographics, clinical manifestation, past history, serology test results, MRI features, pathology, treatment modality and prognosis of four patients were collected and analyzed.
Results
Three of four patients had no clinical symptoms, while one patient's symptom was abdominal pain. The levels of tumor markers of all four patients were within the normal reference range and serological tests were negative for hepatitis B and C virus. The MRI imaging findings of all four patients were mixed mass with highly disordered vascular characteristics. All four patients were misdiagnosed preoperatively. One patient who underwent hepatic lobectomy was still alive for about 18 months after surgery. One patient who underwent hepatic lobectomy has survived for only 6 months due to severe pneumonia. The other two patients who received transarterial chemoembolization survived 16 months and 11 months respectively.
Conclusion
The clinical symptoms of primary hepatic angiosarcoma are not typical, and primary hepatic angiosarcoma is easily misdiagnosed. The typical imaging manifestations are structural disorder and heterogeneous tumor. Hepatic lobectomy and transarterial chemoembolization may be important surgical treatments to improve the prognosis of patients.
Impacts of the aquaculture on the distribution of dissolved organic matter in the coastal Jeju Island, Korea, revealed by absorption and fluorescence spectroscopy
Authors: Kim Jeonghyun , Kim Yeseul , Park Sung Eun , Kim Tae-Hoon , Kim Bong-Guk , Kang Dong-Jin , Rho TaeKeun 
Published: February 2021

Abstract
To demonstrate behavior of dissolved organic matter (DOM) derived from coastal aquaculture, dissolved organic carbon (DOC), total dissolved nitrogen (TDN), chromophoric dissolved organic matter (CDOM), and fluorescent dissolved organic matter (FDOM) were measured around the coastal Jeju Island, Korea. As reported by previous studies, pristine groundwater with extremely depleted DOC (< 30 µM) has been used as culturing water in the coastal aquafarms. However, the concentration of DOC within 1.5 km from the discharge outlet of the aquafarms was approximately two times higher than that in the groundwater. In addition, the concentration of TDN exponentially increased close to the discharge outlet. These distribution patterns indicate the aquafarm is a significant DOM source. Herein, principal component analysis including the absorption coefficient (a350), spectral slope coefficient (S250 − 600), specific UV absorbance (SUVA254), and five fluorescent components were applied to categorize DOM origins. We found two distinct groups: aquaculture activity for TDN with high molecular weights and natural biological activity for DOC enrichment. Our study has also critical implications for the efficient monitoring of anthropogenic organic pollutant from aquafarms using unique optical signals.
SARS-CoV-2 RBD in vitro evolution parrots and predicts contagious mutation spread
Authors: Schreiber Gideon , Zahradník Jiri , Marciano Shir , Shemesh Maya , Zoler Eyal , Chiaravalli Jeanne , Meyer Bjoern , Rudich Yinon , Dym Orly , Elad Nadav 
Published: February 2021

Abstract
SARS-CoV-2 is continually evolving, with more contagious mutations spreading rapidly. Using in vitro evolution to affinity maturate the receptor-binding domain (RBD) of the spike protein towards ACE2 resulted in the more contagious mutations, S477N, E484K, and N501Y, to be among the first selected, explaining the convergent evolution of the “European” (20E-EU1), “British” (501.V1),”South African” (501.V2), and Brazilian variants (501.V3). Plotting the binding affinity to ACE2 of all RBD mutations against their incidence in the population shows a strong correlation between the two. Further in vitro evolution enhancing binding by 600-fold provides guidelines towards potentially new evolving mutations with even higher infectivity. For example, Q498R epistatic to N501Y. Nevertheless, the high-affinity RBD is also an efficient drug, inhibiting SARS-CoV-2 infection. The 2.9Å Cryo-EM structure of the high-affinity complex, including all rapidly spreading mutations, provides a structural basis for future drug and vaccine development and for in silico evaluation of known antibodies.
An Unusual Case of Chest Wall Glomus Tumor Presenting With Axillary Pain: a Case Report and Literature Review
Authors: Zanjani Leila Oryadi , Nia Bahman Shafiee , Vosoughi Farzad , Mirzaian Elham , Aghaghazvini Leila , Aidin Arabzadeh 
Published: February 2021

Abstract
Background: Glomus tumor is an uncommon soft tissue tumor. However, as the tumor causes significant disability, its early diagnosis is essential. It involves subungual areas of fingers and toes in most cases, and its extra-digital involvement is rarely seen. To the best of the authors' knowledge, only a few chest wall involvement cases have been reported in the literature. Case presentation: In this paper, we describe a 63-year-old patient with a chest wall glomus tumor presented with axillary paroxysmal pain and limitation in his shoulder range of motion that had been missed for nearly 15 years. His symptoms were relieved immediately following surgical excision.Conclusion: Glomus tumors may involve any part of the human body. It is curable with surgical excision in most cases. Therefore, a correct early diagnosis has paramount importance. A high index of suspicion is needed for early diagnosis, especially when the tumor involves uncommon anatomic areas.
Clinical Characteristics of SARS-CoV-2&nbsp;by Re-infection Vs. Reactivation: A Case Series From Iran
Authors: Salehi-Vaziri Mostafa , Jalali Tahmineh , Farahmand Behrokh , Fotouhi Fatemeh , Banifazl Mohammad , Pouriayevali Mohammad Hassan , Larijani Mona Sadat , Afzali Neda , Ramezani Amitis 
Published: February 2021

Abstract
Purpose: COVID-19 immunity in infected individuals may not be persistent. The specific response wanes in patients who have recovered from this infection. Nevertheless, it has not been fully understood whether true re-infection occurs or the viral reactivation.Methods: In this study, we investigated three COVID-19 patients who represented the symptoms after recovery. Chest CT scan was applied to assess the patients along with the viral samples from oropharyngeal/nasopharyngeal which were subjected to RT-PCR. The viral genome sequencing was applied where possible to distinguish possible re-infection or latent reactivation. Moreover, COVID-19 specific antibodies available data were evaluated in each incidence.Results: The second episode of SARS-CoV-2 infection was different among the investigated subjects who experienced an interval between positive PCR tests ranged between 63 and 156 days. The disease presentation was less or more severe in the second infection. All cases were found IgG positive in the re-infection phase. The sequencing of SARS-CoV-2 sample obtained from two cases revealed a D614G mutation of S gene from the second isolated sample strengthens the case for the re-infection. Conclusions: The possibility of re-infection and reactivation could have significant effect on clinical implications and also vaccination. Our data supports clear warning of SARS-CoV-2 continuous circulation potency among the populations in spite of herd immunity either with natural infection or vaccination. This issue is critical in term of the patients, clinical investigate and viral transmission.