bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mitochondrial alternative oxidase contributes to successful tardigrade anhydrobiosis
Authors: Wojciechowska Daria , Karachitos Andonis , Roszkowska Milena , Rzeźniczak Wiktor , Sobkowiak Robert , Kaczmarek Łukasz , Kosicki Jakub Z. , Kmita Hanna 
Published: April 2021

AbstractAnhydrobiosis can be described as an adaptation to lack of water that enables some organisms, including tardigrades, to survive extreme conditions, even some that do not exist on Earth. The cellular mechanisms underlying anhydrobiosis are still not completely explained including the putative contribution of mitochondrial proteins. Since mitochondrial alternative oxidase (AOX), described as a drought response element in plants, was recently proposed for various invertebrates including tardigrades, we investigated whether AOX is involved in successful anhydrobiosis of tardigrades. Milnesium inceptum was used as a model for the study. We confirmed functionality of M. inceptum AOX and estimated its contribution to the tardigrade revival after anhydrobiosis of different durations. We observed that AOX activity was particularly important for M. inceptum revival after the long-term tun stage but did not affect the rehydration stage specifically. The results may contribute to our understanding and then application of anhydrobiosis underlying mechanisms.
The value of serum progesterone level on day of human chorionic gonadotrophin administration / metaphase II oocyte ratio in predicting IVF/ICSI outcome in patients with normal ovarian reserve
Authors: Mahran Ahmad , Khairy Mohammed , Elkhateeb Reham , Hegazy Abdel Rahman , Abdelmeged Ayman , Batiha Gaber El-Saber , Alsharif Khalaf F. , Hetta Helal F. , Bahaa Haitham Ahmed 
Published: April 2021

Abstract
Background
The clinical implication of the increased serum progesterone level on the day of HCG administration in assisted reproduction treatment (ART) is still controversial. The current study aimed to compare the predictive value of serum progesterone on day of HCG administration / metaphase II oocyte (P/MII) ratio on IVF/ ICSI outcome to serum progesterone (P) level alone and the ratio of serum progesterone/estradiol level (P/E2) ratio in prediction of pregnancy rates after ART.

Material & methods
Two hundred patients admitted to the IVF/ICSI program at Minia IVF center in Egypt in the period from October 2016 to May 2018 were included in this study. Serum Progesterone (P) and Estradiol (E2) levels were estimated on the day of HCG administration. The ratio between serum P and the number of MII oocytes (P/MII ratio) was calculated and the predictive values of the three parameters (P, P/E2 ratio and P/MII ratio) in prediction of cycle outcomes were measured.

Results
P/ MII oocyte ratio was significantly lower in patients who attained clinical pregnancy (n = 97) as compared with those who couldn’t whilst there was no significant difference in P and P/E2 ratio between the two groups. Using a cut off value of 0.125, the sensitivity and specificity of progesterone/ MII ratio in prediction of no pregnancy in IVF/ICSI were 75.7 and 77.1% respectively with the area under The Receiver operating curve (ROC-AUC) = 0.808. The respective values of the ROC-AUC for the P and P/E2 ratio were 0.651 and 0.712 with sensitivity and specificity of 71.2 and 73.5%for P level and of 72.5 and 75.3% for P/E2 ratio. Implantation or clinical pregnancy rates were significantly different between patients with high and low P/MII ratio irrespective of day of embryo transfer (day 3 or 5).

Conclusions
In patients with normal ovarian response, serum progesterone on day of HCG / MII oocyte ratio can be a useful predictor of pregnancy outcomes and in deciding on freezing of all embryos for later transfer instead of high progesterone level alone.
A clinical and cost-effectiveness trial of a parent group intervention to manage challenging restricted and repetitive behaviours in young children with autism spectrum disorder: study protocol for a randomised controlled trial
Authors: Grahame Victoria , Dixon Linda , Fletcher-Watson Sue , Garland Deborah , Glod Magdalena , Goodwin Jane , Grayson Zoe , Heron Saoirse , Honey Emma , Iversen Rebecca , Kasim Adetayo S. , Kernohan Ashleigh , Kharatikoopaei Ehsan , Le Couteur Ann , Mackie Leila , Mathias Ayesha , Probert Helen , Riby Deborah , Rob Priyanka , Rogan Leanne , Thompson Sarah , Vale Luke , Walls Eamonn , Webb Elspeth Imogen , Weetman Christopher , Wolstenhulme Faye , Wood Ruth , Rodgers Jacqui 
Published: April 2021

Abstract
Background
Restricted and repetitive behaviours vary greatly across the autism spectrum, and although not all are problematic some can cause distress and interfere with learning and social opportunities. We have, alongside parents, developed a parent group based intervention for families of young children with autism, which aims to offer support to parents and carers; helping them to recognise, understand and learn how to respond to their child’s challenging restricted repetitive behaviours.

Methods
The study is a clinical and cost-effectiveness, multi-site randomised controlled trial of the Managing Repetitive Behaviours (MRB) parent group intervention versus a psychoeducation parent group Learning About Autism (LAA) (n = 250; 125 intervention/125 psychoeducation; ~ 83/site) for parents of young children aged 3–9 years 11 months with a diagnosis of autism.
All analyses will be done under intention-to-treat principle. The primary outcome at 24 weeks will use generalised estimating equation (GEE) to compare proportion of children with improved RRB between the MRB group and the LAA group. The GEE model will account for the clustering of children by parent groups using exchangeable working correlation. All secondary outcomes will be analysed in a similar way using appropriate distribution and link function.
The economic evaluation will be conducted from the perspective of both NHS costs and family access to local community services. A ‘within trial’ cost-effectiveness analysis with results reported as the incremental cost per additional child achieving at least the target improvement in CGI-I scale at 24 weeks.

Discussion
This is an efficacy trial to investigate the clinical and cost-effectiveness of a parent group based intervention designed to help parents understand and manage their child’s challenging RRB. If found to be effective, this intervention has the potential to improve the well-being of children and their families, reduce parental stress, greatly enhance community participation and potential for learning, and improve longer-term outcomes.

Trial registration
Trial ID: ISRCTN15550611 Date registered: 07/08/2018. Sponsor and Monitor: Cumbria, Northumberland, Tyne and Wear NHS Foundation Trust R&D Manager Lyndsey Dixon, Address: St Nicholas Hospital, Jubliee Road, Gosforth, Newcastle upon Tyne NE3 3XT, lyndsey.dixon@cntw.nhs.uk, Tel: 0191 246 7222
Tactile cortical responses and association with tactile reactivity in young children on the autism spectrum
Authors: Espenhahn Svenja , Godfrey Kate J. , Kaur Sakshi , Ross Maia , Nath Niloy , Dmitrieva Olesya , McMorris Carly , Cortese Filomeno , Wright Charlene , Murias Kara , Dewey Deborah , Protzner Andrea B. , McCrimmon Adam , Bray Signe , Harris Ashley D. 
Published: April 2021

Abstract
Background
Unusual behavioral reactions to sensory stimuli are frequently reported in individuals on the autism spectrum (AS). Despite the early emergence of sensory features (< age 3) and their potential impact on development and quality of life, little is known about the neural mechanisms underlying sensory reactivity in early childhood autism.

Methods
Here, we used electroencephalography (EEG) to investigate tactile cortical processing in young children aged 3–6 years with autism and in neurotypical (NT) children. Scalp EEG was recorded from 33 children with autism, including those with low cognitive and/or verbal abilities, and 45 age- and sex-matched NT children during passive tactile fingertip stimulation. We compared properties of early and later somatosensory-evoked potentials (SEPs) and their adaptation with repetitive stimulation between autistic and NT children and assessed whether these neural measures are linked to “real-world” parent-reported tactile reactivity.

Results
As expected, we found elevated tactile reactivity in children on the autism spectrum. Our findings indicated no differences in amplitude or latency of early and mid-latency somatosensory-evoked potentials (P50, N80, P100), nor adaptation between autistic and NT children. However, latency of later processing of tactile information (N140) was shorter in young children with autism compared to NT children, suggesting faster processing speed in young autistic children. Further, correlational analyses and exploratory analyses using tactile reactivity as a grouping variable found that enhanced early neural responses were associated with greater tactile reactivity in autism.

Limitations
The relatively small sample size and the inclusion of a broad range of autistic children (e.g., with low cognitive and/or verbal abilities) may have limited our power to detect subtle group differences and associations. Hence, replications are needed to verify these results.

Conclusions
Our findings suggest that electrophysiological somatosensory cortex processing measures may be indices of “real-world” tactile reactivity in early childhood autism. Together, these findings advance our understanding of the neurophysiological mechanisms underlying tactile reactivity in early childhood autism and, in the clinical context, may have therapeutic implications.
Sodium alginate-bioglass-encapsulated hAECs restore ovarian function in premature ovarian failure by stimulating angiogenic factor secretion
Authors: Huang Yating , Ma Zhijie , Kuang Xiaojun , Zhang Qiuwan , Li Haiyan , Lai Dongmei 
Published: April 2021

AbstractBackgroundHuman amniotic epithelial cells (hAECs) exhibit a strong capability to restore ovarian function in chemotherapy-induced premature ovarian failure (POF). However, the therapeutic efficacy of hAECs is usually affected by the limited number and proliferative ability of grafted hAECs in target organs. The transplantation of stem cells encapsulated in sodium alginate-bioglass (SA-BG) composite hydrogel has recently been shown to be an effective strategy for tissue regeneration. The current study aims to investigate the therapeutic potential of hAECs or hAEC-derived conditioned medium (CM) encapsulated in SA-BG in mice with chemotherapy-induced POF.MethodsC57BL/6 mice were intraperitoneally injected with chemotherapy drugs to induce POF. hAECs or CM were harvested and encapsulated in SA-BG composite hydrogel, which were transplanted onto the injured ovaries of mice with POF. Follicle development, granulosa cell function, and ovarian angiogenesis were evaluated by morphological methods. To further elucidate the effect of SA-BG-encapsulated hAECs/CM on vascularization, the tube formation of human umbilical vein epithelial cells (hUVECs) was conducted in vitro. Cytokine array and ELISA were used to analyze and quantify the effects of bioactive components released by SA-BG on the secretion of angiogenic factors by hAECs.ResultsThe transplantation of SA-BG-encapsulated hAECs/CM restored follicle development, repaired granulosa cell function, and enhanced ovarian angiogenesis in POF mice. The further study showed that SA-BG significantly promoted the tube formation of hUVECs in vitro. Moreover, encapsulating hAECs could facilitate the effect of SA-BG on inducing the formation of the capillary tube in a paracrine manner. In addition, we found that SA-BG extracts significantly enhanced the viability of hAECs and stimulated the secretion of pro-angiogenic factors of hAECs. Notably, compared with SA-BG/CM, SA-BG/hAECs achieve better therapeutic effects, possibly because stimulation of BG enhanced the viability and paracrine capacity of hAECs.ConclusionsThe present study initially demonstrates that SA-BG-encapsulated hAECs or CM can exert a therapeutic effect on chemotherapy-induced POF mainly by protecting granulosa cell function and enhancing ovarian vascularization, which might provide a novel strategy for the delivery of hAECs for treating POF.
Regulatory roles and mechanisms of alternative RNA splicing in adipogenesis and human metabolic health
Authors: Chao Yunqi , Jiang Yonghui , Zhong Mianling , Wei Kaiyan , Hu Chenxi , Qin Yifang , Zuo Yiming , Yang Lili , Shen Zheng , Zou Chaochun 
Published: April 2021

AbstractAlternative splicing (AS) regulates gene expression patterns at the post-transcriptional level and generates a striking expansion of coding capacities of genomes and cellular protein diversity. RNA splicing could undergo modulation and close interaction with genetic and epigenetic machinery. Notably, during the adipogenesis processes of white, brown and beige adipocytes, AS tightly interplays with the differentiation gene program networks. Here, we integrate the available findings on specific splicing events and distinct functions of different splicing regulators as examples to highlight the directive biological contribution of AS mechanism in adipogenesis and adipocyte biology. Furthermore, accumulating evidence has suggested that mutations and/or altered expression in splicing regulators and aberrant splicing alterations in the obesity-associated genes are often linked to humans’ diet-induced obesity and metabolic dysregulation phenotypes. Therefore, significant attempts have been finally made to overview novel detailed discussion on the prospects of splicing machinery with obesity and metabolic disorders to supply featured potential management mechanisms in clinical applicability for obesity treatment strategies.
Ambient backscatter communication-based smart 5G IoT network
Authors: Liu Qiang , Sun Songlin , Yuan Xueguang , Zhang Yang’an 
Published: April 2021

AbstractIn this paper, we propose an ambient backscatter communication-based smart 5G IoT network. The network consists of two parts, namely a real-time data transmission system based on ambient backscatter communication and a real-time big data analysis system based on the combination of shallow neural networks and deep neural networks. The real-time data transmission system based on ambient backscatter communication can extend the standby time of data collection equipment, reduce the size of the equipment, and increase the comfort of wearing. The real-time big data analysis system combining the shallow neural network and the deep neural network can greatly reduce the pressure caused by the frequent deep neural network calculations of the MEC and greatly reduce the energy consumed by the MEC for remote real-time monitoring.
Convergence analysis of a general inertial projection-type method for solving pseudomonotone equilibrium problems with applications
Authors: Rehman Habib ur , Kumam Poom , Gibali Aviv , Kumam Wiyada 
Published: April 2021

AbstractIn this paper, we introduce a new algorithm by incorporating an inertial term with a subgradient extragradient algorithm to solve the equilibrium problems involving a pseudomonotone and Lipschitz-type continuous bifunction in real Hilbert spaces. A weak convergence theorem is well established under certain mild conditions for the bifunction and the control parameters involved. Some of the applications to solve variational inequalities and fixed point problems are considered. Finally, several numerical experiments are performed to demonstrate the numerical efficacy and superiority of the proposed algorithm over other well-known existing algorithms.
A call for consistency with the terms ‘wetter’ and ‘drier’ in climate change studies
Authors: Roth Nina , Jaramillo Fernando , Wang-Erlandsson Lan , Zamora David , Palomino-Ángel Sebastián , Cousins Sara A. O. 
Published: April 2021

AbstractOngoing and future hydroclimatic changes have large environmental and societal impacts. In terrestrial ecosystems, these changes are usually described with the terms ‘wetter’ and ‘drier’, which refer to the change in the quantity and/or presence of water, either as water fluxes or stocks. We conducted a literature review of almost 500 recent climate change studies to quantitatively investigate the consistency of the use of these terms across disciplines, regarding the hydroclimatic variables they are related to. We found that although precipitation is prevalently used to describe ‘wetter’ and ‘drier’ conditions, many other variables are also used to refer to changes in water availability between research fields, pointing to a varied perspective on the use of these terms. Some studies do not define the terms at all. In order to facilitate meta-analyses across disciplines, we therefore highlight the need to explicitly state which hydroclimatic variables authors are referring to. In this way, we hope that the terms ‘wetter’ and ‘drier’ used in scientific studies are easier to relate to hydroclimatic processes, which should facilitate the application by authorities and policy makers.
Crustal architecture of a metallogenic belt and ophiolite belt: implications for mineral genesis and emplacement from 3-D electrical resistivity models (Bayankhongor area, Mongolia)
Authors: Comeau Matthew J. , Becken Michael , Kuvshinov Alexey V. , Demberel Sodnomsambuu 
Published: April 2021

AbstractCrustal architecture strongly influences the development and emplacement of mineral zones. In this study, we image the crustal structure beneath a metallogenic belt and its surroundings in the Bayankhongor area of central Mongolia. In this region, an ophiolite belt marks the location of an ancient suture zone, which is presently associated with a reactivated fault system. Nearby, metamorphic and volcanic belts host important mineralization zones and constitute a significant metallogenic belt that includes sources of copper and gold. However, the crustal structure of these features, and their relationships, are poorly studied. We analyze magnetotelluric data acquired across this region and generate three-dimensional electrical resistivity models of the crustal structure, which is found to be locally highly heterogeneous. Because the upper crust (< 25 km) is found to be generally highly resistive (> 1000 Ωm), low-resistivity (< 50 Ωm) features are conspicuous. Anomalous low-resistivity zones are congruent with the suture zone, and ophiolite belt, which is revealed to be a major crustal-scale feature. Furthermore, broadening low-resistivity zones located down-dip from the suture zone suggest that the narrow deformation zone observed at the surface transforms to a wide area in the deeper crust. Other low-resistivity anomalies are spatially associated with the surface expressions of known mineralization zones; thus, their links to deeper crustal structures are imaged. Considering the available evidence, we determine that, in both cases, the low resistivity can be explained by hydrothermal alteration along fossil fluid pathways. This illustrates the pivotal role that crustal fluids play in diverse geological processes, and highlights their inherent link in a unified system, which has implications for models of mineral genesis and emplacement. The results demonstrate that the crustal architecture—including the major crustal boundary—acts as a first‐order control on the location of the metallogenic belt.
Magnesium sulfate intravenous infusion versus intrathecal injection for prevention of post-spinal shivering during lower limb fracture surgery: a randomized controlled study
Authors: Mohamed Sherif Abdullah , El Mekawy Nevan Mohamed , Abdelfattah Raed Mohamed , Elsonbaty Ahmed Ibrahim Abdelkhalik , Elsonbaty Mohamed Ibrahim Abdelkhalik 
Published: April 2021

AbstractBackgroundShivering is a prevalent adverse event after spinal anesthesia, often disturbing to medical staff and hazardous to patients. Intravenous magnesium sulfate [MgSO4] has been proven to be effective in prevention of post-spinal shivering. However, the risk of intravenous route to develop hypermagnesemia in certain patients encouraged us to do this study in order to investigate the effectiveness of intrathecal MgSO4as an alternative to the intravenous route in prevention of post-spinal shivering. The enrolled 135 patients were allocated to 3 groups, 45 patients each, groups: intrathecal [T], IV MgSO4infusion [M], and control [C]. Group T (no. = 45) received 50 mg MgSO4added to heavy bupivacaine 0.5% intrathecally, while group M (no. = 45) received IV MgSO4as 50 mg/kg in 100 cc saline within 20 min as a loading dose then infused as 2 mg/kg/h after performing spinal anesthesia. The vital signs, duration and shivering grades, temperature, and adverse effects were recorded.ResultsT and M groups showed a significant difference from control as regard the incidence of shivering with 40%, 26.7%, and 64.4% for T, M, and C groups, respectively, patients needed rescue pethidine were 17.7%, 11.1%, and 60% for group T, M, and C, respectively, and shivering duration (min) was 24.86 ± 7.411, 20.47 ± 6.61, and 45 ± 36.2 for groups T, M, and C, respectively with a statistically insignificant difference between group T and M as regards these parameters.ConclusionsIntrathecal MgSO4has the potentiality to be used as an alternative to IV route regarding prevention of post-spinal shivering. This makes advantage for intrathecal magnesium use in patients at risk for magnesium toxicity.
Trialists perspectives on sustaining, spreading, and scaling-up of quality improvement interventions
Authors: Laur Celia , Corrado Ann Marie , Grimshaw Jeremy M. , Ivers Noah 
Published: April 2021

AbstractBackgroundQuality improvement (QI) evaluations rarely consider how a successful intervention can be sustained long term, nor how to spread or scale to other locations. A survey of authors of randomized trials of diabetes QI interventions included in an ongoing systematic review found that 78% of trials reported improved quality of care, but 40% of these trials were not sustained. This study explores why and how the effective interventions were sustained, spread, or scaled.MethodsA qualitative approach was used, focusing on case examples. Diabetes QI program trial authors were purposefully sampled and recruited for telephone interviews. Authors were eligible if they had completed the author survey, agreed to follow-up, and had a completed a diabetes QI trial they deemed “effective.” Snowball sampling was used if the participant identified someone who could provide a different perspective on the same trial. Interviews were transcribed verbatim. Inductive thematic analysis was conducted to identify barriers and facilitators to sustainability, spread, and/or scale of the QI program, using case examples to show trajectories across projects and people.ResultsEleven of 44 eligible trialists participated in an interview. Four reported that the intervention was “sustained” and nine were “spread,” however, interviews highlighted that these terms were interpreted differently over time and between participants. Participant stories highlighted the varied trajectories of how projects evolved and how some research careers adapted to increase impact. Three interacting themes, termed the “3C’s,” helped explain the variation in sustainability, spread, and scale: (i) understanding theconceptsof implementation, sustainability, sustainment, spread, and scale; (ii) having the appropriatecompetencies; and (iii) the need for individual, organizational, and systemcapacity.ConclusionsChallenges in defining sustainability, spread and scale make it difficult to fully understand impact. However, it is clear that from the beginning of intervention design, trialists need to understand the concepts and have the competency and capacity to plan for feasible and sustainable interventions that have potential to be sustained, spread and/or scaled if found to be effective.