bluuebunny/crossref_metadata_2025_split huggingface dataset:
Polymerase theta-helicase promotes end joining by stripping single-stranded DNA-binding proteins and bridging DNA ends
Authors: Schaub Jeffrey M. , Soniat Michael M. , Finkelstein Ilya J. 
Published: June 2021

AbstractHomologous recombination-deficient cancers rely on DNA polymerase Theta (Polθ)-Mediated End Joining (TMEJ), an alternative double-strand break repair pathway. Polθ is the only vertebrate polymerase that encodes an N-terminal superfamily 2 (SF2) helicase domain, but the role of this helicase domain in TMEJ remains unclear. Using single-molecule imaging, we demonstrate that Polθ-helicase (Polθ-h) is a highly processive single-stranded DNA (ssDNA) motor protein that can efficiently strip Replication Protein A (RPA) from ssDNA. Polθ-h also has a limited capacity for disassembling RAD51 filaments but is not processive on doublestranded DNA. Polθ-h can bridge two non-complementary DNA strands in trans. PARylation of Polθ-h by PARP-1 resolves these DNA bridges. We conclude that Polθ-h removes RPA and RAD51 filaments and mediates bridging of DNA overhangs to aid in polymerization by the Polθ polymerase domain.
Single nuclei transcriptome of the Lesser Duckweed Lemna minuta reveals cell trajectories for an entire plant
Authors: Abramson Bradley W. , Novotny Mark , Hartwick Nolan T. , Colt Kelly , Aevermann Brian D. , Scheuermann Richard H. , Michael Todd P. 
Published: June 2021

AbstractThe ability to trace every cell in some model organisms has led to the fundamental understanding of development and cellular function. However, in plants the complexity of cell number, organ size and developmental times makes this a challenge even in the diminutive model plant Arabidopsis thaliana. Here we develop the Lesser Duckweed Lemna minuta as a model with a reduced body plan, small genome size and clonal growth pattern that enables simultaneous tracing of cells from the entire plant over the complete developmental cycle. We generated a chromosome-resolved genome for the 360 megabase genome and defined the growth trajectory of the entire plant with single nuclei RNA sequencing. The L. minuta gene complement represents a primarily non-redundant set with only the ancient tau whole genome duplication shared with all monocots, and paralog expansion as a result of tandem duplications related to phytoremediation. Thirteen distinct cell types representing meristem, the leaf-stem fusion called a frond, and root-like tissues were defined using gene orthology with single cell expression from model plants, gene ontology categories, and cell trajectory analysis. Dividing meristem cells give rise to two main branches of root-transition and mesophyll cells, which then give rise to terminally differentiated parenchyma, epidermal and root cells. Mesophyll tissues express high levels of elemental transport genes consistent with this tissue playing a role in L. minuta wastewater detoxification. The L. minuta genome and cell map provide a paradigm to decipher developmental genes and pathways for an entire plant.Sentence summaryGenome and single nuclei transcriptome of the Lesser Duckweed Lemna minuta enables tracing of all developmental, transitional and terminal cells of an entire plant.
Usefulness of the budget: the mediating effect of participative budgeting and budget-based evaluation and rewarding
Authors: Wagner Jaroslav , Petera Petr , Popesko Boris , Novák Petr , Šafr Karel 
Published: June 2021

PurposeThis paper contributes to budgeting-related literature by investigating whether the participation of operational managers in budgeting, and budget-based evaluations and the rewarding of operational managers, significantly mediate the relationship between budget use for operational management and the perceived usefulness of the budget.Design/methodology/approachThe paper is based on data gathered from a survey of Czech medium- and large-sized companies from the manufacturing sector. The hypothesised relationships are tested using partial least squares structural equation modelling (PLS-SEM).FindingsOverall usefulness of the budget, as perceived by principals (top managers), is positively influenced by the scope of budget use, but, more importantly, the positive mediating effects of participative budgeting and budget-based evaluation and rewarding on this relationship are significant and strong.Research limitations/implicationsThe subjective perceptions of respondents were investigated with the understanding that they may not represent actual situations in their organisations. Companies with well-functioning budgeting systems were more likely to take part in the research. Regarding satisfaction, the authors studied the perceived usefulness of the budget. Only medium- and large-sized manufacturing companies located in a post-communist country were analysed and generalisations should, therefore, be taken with caution.Practical implicationsThe results in the studied sample indicate that satisfaction with budgeting is positively correlated with the rewarding and evaluation of operational managers, and with enabling the participation of operational managers in preparing and updating their budgets.Originality/valueThis research contributes to prior literature on budgeting by investigating the mediating effects of the participation of operational managers in budgeting, and the budget-based evaluation and rewarding of operational managers on the perceived usefulness of the budget by principals in an integrated model using the PLS-SEM approach.
How do subordinates react to perceived narcissistic supervision? The roles of perceived interactional justice and need for belonging
Authors: Ni Dan , Liu Xin , Zheng Xiaoming 
Published: June 2021

PurposeThis paper aims to examine how and when perceived narcissistic supervision influences subordinates' work engagement and counterproductive work behavior (CWB) based on organizational justice theory.Design/methodology/approachTwo-wave data were collected from 320 employees in a Chinese manufacturing company.FindingsSubordinates' perceived interactional justice mediated the relationships between perceived narcissistic supervision and work engagement and CWB. Higher levels of subordinates' need for belonging strengthened the detrimental impacts of perceived narcissistic supervision on subordinates' outcomes.Practical implicationsOrganizations should reduce the occurrence of narcissistic supervision, enhance subordinates' perception of interactional justice and pay more attention to subordinates' need for belonging in personnel and team arrangement.Originality/valueAlthough research has documented the detrimental effects of perceived narcissistic supervision, little effort has been made to investigate how such effects occur and which factors might amplify such effects. This study identified the mechanism underlying the link between perceived narcissistic supervision and subordinates' outcomes and unpacked the moderating role of subordinates' need for belonging.
Praised from birth: social approval assets in the creation of a new university
Authors: Aula Hannamari , Siltaoja Marjo 
Published: June 2021

PurposeThe authors explore how social approval assets, namely status and reputation, are used to legitimate and categorise a new national university. They argue that in the course of the legitimation process, status and reputation work as stakeholder-oriented value-creating benefits. The authors specifically analyse the discursive constructions and labels used in the process and how the process enables nationwide university reform.Design/methodology/approachThe authors’ longitudinal case study utilises critical discourse analysis and analyses media and policy discourses regarding the birth of Aalto University.FindingsThe findings suggest that the legitimation of the new university was accomplished through the use of two distinct discourses: one on higher education and another on the market economy. These discourses not only sought to legitimise the new university as categorically different from existing Finnish universities, but also rationalised the merger using the expected reputation and status benefits that were claimed would accrue for supporters.Practical implicationsThis study elaborates on the role of various social approval assets and labels in legitimation processes and explores how policy enforcement can take place in arenas that are not necessarily perceived as policymaking. For managers, it is crucial to understand how a chosen label (name) can result in both stakeholder support and resistance, and how important it is to anticipate the changes a label can invoke.Originality/valueThe authors propose that the use of several labels regarding a new organisation is strategically beneficial to attracting multiple audiences who may hold conflicting interests in terms of what the organisation and its offerings should embody. They propose that even though status and reputation have traditionally been defined as possessions of an organisation, they should be further understood as concepts used to disseminate and justify the interests, norms, structures and values in a stakeholder network.
Mixed qualitative–quantitative approach for bidding decisions in construction
Authors: Mohamed Emad , Jafari Parinaz , Hammad Ahmed 
Published: June 2021

PurposeThe bid/no-bid decision is critical to the success of construction contractors. The factors affecting the bid/no-bid decision are either qualitative or quantitative. Previous studies on modeling the bidding decision have not extensively focused on distinguishing qualitative and quantitative factors. Thus, the purpose of this paper is to improve the bidding decision in construction projects by developing tools that consider both qualitative and quantitative factors affecting the bidding decision.Design/methodology/approachThis study proposes a mixed qualitative-quantitative approach to deal with both qualitative and quantitative factors. The mixed qualitative-quantitative approach is developed by combining a rule-based expert system and fuzzy-based expert system. The rule-based expert system is used to evaluate the project based on qualitative factors and the fuzzy expert system is used to evaluate the project based on the quantitative factors in order to reach the comprehensive bid/no-bid decision.FindingsThree real bidding projects are used to investigate the applicability and functionality of the proposed mixed approach and are tested with experts of a construction company in Alberta, Canada. The results demonstrate that the mixed approach provides a more reliable, accurate and practical tool that can assist decision-makers involved in the bid/no-bid decision.Originality/valueThis study contributes theoretically to the body of knowledge by (1) proposing a novel approach capable of modeling all types of factors (either qualitative or quantitative) affecting the bidding decision, and (2) providing means to acquire, store and reuse expert knowledge. Practical contribution of this paper is to provide decision-makers with a comprehensive model that mimics the decision-making process and stores experts' knowledge in the form of rules. Therefore, the model reduces the administrative burden on the decision-makers, saves time and effort and reduces bias and human errors during the bidding process.
Comparative analysis of the relationship between internal control weakness and different types of auditor opinions in fraudulent and non-fraudulent firms
Authors: Lari Dashtbayaz Mahmoud , Salehi Mahdi , Hedayatzadeh Mahdi 
Published: June 2021

Purpose
This study aims to assess the relationship between internal control weakness and different types of auditor opinions in fraudulent and non-fraudulent firms. The study's main objective is to investigate fraud in business firms and analyze internal controls and types of proposed opinions by the auditor about his desired firm. The outbreak of fraud in firms is of utmost importance to a broad spectrum of society. Internal controls and the auditor's role in preventing and detecting frauds should not be taken for granted.

Design/methodology/approach
The present study's statistical population includes 179 listed firms on the Stock Exchange selected as the study sample using the systematic elimination method during 2012–2019. As the study's dependent variable (the type of auditor’s opinion), research hypotheses were analyzed using the Logit regression model.

Findings
The results show that the relationship between internal control weakness and opinion type is significantly different in fraudulent and non-fraudulent firms. Moreover, the relationship between internal control weakness and type of auditor opinion in fraudulent firms and the relationship between internal control weakness and type of auditor opinion in non-fraudulent firms are significant.

Originality/value
By assessing the related literature, the authors have found no study to directly assess the comparative relationship between internal control weakness and the type of auditor opinion, which can be named as the main objective of the study.
Sustainable management of agriculture products value chain in responses to climate change for South-Eastern coast of Bangladesh
Authors: Barua Prabal , Rahman Syed Hafizur , Barua Maitri 
Published: June 2021

PurposeThis paper is designed to assess the sustainable value chain approaches for marketing channel development opportunities for agricultural products in coastal Bangladesh to combat climate change through an approach of community-based adaptation options.Design/methodology/approachThe study was designed to select the potential value chain candidate and to analyze and establish a value chain map to benefit the crop farmers. In this connection, the resources of the whole context were evaluated. The approach uses few tools to generate three outputs, the last of which are the final list of value chains selected for in-depth assessment to design interventions as community-based adaptation practices of the study to combat climate change in the study areas.FindingsThe study demonstrated that the difference in the institutional circumstances of the end markets of the agriculture products is connected to the different categories of harmonization and control of the facilitating environment throughout the supply chains. National and local networks improve the value chain in terms of the value addition of the agriculture products, technology improvement, market access and profitability of the products. Strengthening the weak financial structure, focus more on formal financial systems and resolving sociocultural and climate change-induced hazard concerns are the major concerns on the development of value chains in the countries. Apparently, guarantee for good governance, checking illegal and unregulated market contexts, proper mitigation measures to climate change are some paramount important issues for the sustainable management of livelihood, yield, income and development.Practical implicationsAll kinds of stakeholders of the agriculture product value chain should focus on competitiveness and productivity and look for and exploit multiple ways to add value once initial success has been attained with a single deal. Ensuring sustainability within the value chains is an important feature to cater to the challenges and changing demands of the age.Originality/valueThe study will help to established a sustainable value chain approach in response to climate change, which process will help to existent opportunities for firms to manage the issue of climate risk by codeveloping and employing adaptation options that may be more preferred or accepted by consumers across the entire chain for the sustainable management of livelihood, yield, income and development.
Why this realtor®? Branding at Boyes Group Realty Inc. version: 2020-10-07
Authors: Klassen Brooke , Lane Brian 
Published: June 2021

Theoretical basis
The case learning objectives and discussion questions relate very closely to several theories related to branding, including brand positioning, brand growth, brand management, customer value proposition, brand matrix and brand identity.

Research methodology
The information provided in this case was gathered by the author through personal interviews and email exchanges with Jordan Boyes, managing broker and owner of Boyes Group Realty Inc. Secondary research was also conducted to gather relevant academic materials, as well as industry and competitor information.

Case overview/synopsis
Jordan Boyes opened Boyes Group Realty Inc. in 2015 after working as a Realtor® at another private realty firm in Saskatoon, Saskatchewan for several years. They offered buying and selling services in the areas of commercial, residential and farm real estate. Over the past five years, they had experienced strong sales numbers and growth in the number of new agents joining the company. However, Boyes saw untapped potential in the marketplace and wanted to develop a brand strategy that took the best advantage of brand extension opportunities to drive the continued growth of his company.

Complexity academic level
This case is suitable at the undergraduate level for a branding course, marketing strategy course or services marketing course. The case is best used to apply the concept of developing brand identity and making strategic decisions in a service-based organization.
Tourism as a bridge for inter-cultural understanding and reconciliation despite the COVID-19 challenges: an interview with Dr Itsik Peres
Authors: Altshuler Alex 
Published: June 2021

Purpose
The interview with Mr Itsik (Isaac) Peres aims to provide a set of insights from the field – through global, Middle-Eastern and Israeli lenses – on some of the major challenges for the tourism industry in the face of COVID-19.

Design/methodology/approach
This paper consists of an academic interview with Mr Itsik (Isaac) Peres and presents an engaging discussion between the experienced and knowledgeable tour guide and a scholar of resilience.

Findings
Mr Peres calls for a balanced approach that views health considerations as the top priority but at the same time believes that the tourism industry may – at least partially – adapt itself to the global pandemic and operate through a “new normal” during COVID-19. It is also claimed that there is no conflict between necessary international standards and local knowledge, creativity and adaptation measures. Mr Itsik Peres also addresses the potential major local and regional opportunities for the tourism industry and for the society as a whole that may emerge because of the new Abraham Accords (a peace treaty between Israel, United Arab Emirates and Bahrain signed in August 2020).

Originality/value
This paper presents an original and comprehensive analysis of the major challenges and solutions for the tourism industry in the face of COVID-19 through the lenses of an experienced professional in the field. The interview provides the reader with short-term and long-term “take away” messages for more effective and adaptive functioning of the tourism industry – during the COVID-19 epidemic and following its hopeful end.
Experimental evaluation of herbicide use on biodiversity, ecosystem services and timber production trade‐offs in forest plantations
Authors: Stokely Thomas D. , Kormann Urs G. , Verschuyl Jake , Kroll Andrew J. , Frey David W. , Harris Scott H. , Mainwaring Doug , Maguire Doug , Hatten Jeff A. , Rivers James W. , Fitzgerald Stephen , Betts Matthew G. 
Published: June 2021

Abstract

The value of non‐commodity ecosystem services provided by forests is widely recognized, but intensive forest management practices are increasing, with uncertain consequences for a multitude of these services. Quantitative relationships among biodiversity conservation, timber production and other ecosystem services remain poorly understood, especially during the early‐successional period of intensively managed forestlands.

We manipulated management intensity in regenerating forest plantations to test the prediction that treatments aimed at maximizing timber production decrease biodiversity conservation and non‐timber services. We measured species richness of 3 taxonomic groups and 13 proxies for provisioning, cultural and regulating services within stands randomly assigned to one of the three herbicide application intensities or an untreated control.

Herbicides increased allocation of net primary production to crop trees, increasing projected timber volume and revenues at 40‐ and 60‐year harvest ages. Commonly used herbicide prescriptions reduced culturally valued plants by 71%, wild‐ungulate forage by 41%, avian richness by 20% and pollinator floral resources by 42%, the latter being associated with 38% fewer pollinator species. However, agriculturally valued bumblebees, pollination of blueberries, avian‐mediated arthropod control, wild ungulate observations and regulation services tied to forest productivity appeared unaffected by increasing management intensity and timber production.

Species richness and flora‐provided services in young forest plantations exhibited strong trade‐offs with projected timber production, whereas post‐treatment vegetation regeneration and site‐level variation likely maintained a range of other services. Although vegetation recovery is important for supporting wildlife and some ecosystem services on industrial forestlands, it is unlikely that any single prescription can optimize both timber and non‐timber benefits to society across managed forest landscapes. Instead, producing different services in discrete portions of the landscape may be necessary.

Synthesis and applications. We tested the effects of intensive forest management via herbicides on ecosystem services and found that biodiversity responses and services from early‐successional vegetation trade‐off against timber production. A number of services appeared to be compatible with timber production, although no single prescription optimized the full range of services. Stand‐level biodiversity conservation and a variety of services could potentially be provided by treatment skips and less‐intensive management on productive sites, although it is unlikely that all services can be optimized without landscape‐level planning.
Rosuvastatin and low‐dose carvedilol combination protects against isoprenaline‐induced myocardial infarction in rats: Role of PI3K/Akt/Nrf2/HO‐1 signalling
Authors: Baraka Sarah A. , Tolba Mai F. , Elsherbini Doaa A. , El‐Naga Reem N. , Awad Azza S. , El‐Demerdash Ebtehal 
Published: June 2021

AbstractRosuvastatin has been shown to activate PI3K/Akt/Nrf2/HO‐1 pathway, which promotes cell survival in the myocardium. This study investigated the therapeutic benefit of adding rosuvastatin to low‐dose carvedilol in protection against myocardial infarction (MI). Rosuvastatin (RSV) and carvedilol (CAR) were given for 7 consecutive days with concurrent administration of two doses of isoprenaline (ISP) on 6th and 7th days to induce MI. Isoprenaline injections caused detrimental alterations in the myocardial architecture and electrocardiogram (ECG) pattern and significantly increased the infarct size, heart index and serum levels of cardiotoxicity markers compared to the control group. ISP induced oxidative damage, inflammatory and apoptotic events and downregulated PI3K/Akt/Nrf2/HO‐1 signalling pathway compared to the control values. Treatment with low‐dose CAR and/or RSV prevented the ECG and histopathological alterations induced by ISP, and also reduced the infarct size, heart index, serum creatine kinase‐MB, cardiac troponin‐I and C‐reactive protein levels compared to ISP group. CAR and/or RSV treatment restored the activity of superoxide dismutase and total antioxidant capacity with a consequent reduction in lipid peroxides level. Further, they decreased the expression of nuclear factor (NF)‐κB (p65) and increased the phosphorylated PI3K and Akt, which may activate the anti‐apoptotic signalling as evidenced by the decreased active caspase 3 level. The combination therapy has a more significant effect in the most studied parameters than their monotherapy, which may be because of the activation of PI3K/Akt Nrf2/HO‐1 pro‐survival signalling pathway. This study highlights the potential benefits of combining RSV with low‐dose CAR in case of MI.