bluuebunny/crossref_metadata_2025_split huggingface dataset:
Adverse reactions of COVID-19 vaccine among frontline workers in Fujairah, UAE
Authors: Al-Worafi Yaser , Ming Long , Alseragi Wafa , Dhabali Abdullah , Al-Shami Abdulkareem 
Published: January 2021

Abstract
This study explored the adverse reactions/effects prevalence, types, duration, and severity of inactivated novel coronavirus pneumonia (COVID-19) vaccine among adult frontline health care professionals and educators. A total of A total of 49 frontline workers in Fujairah, UAE (37 health care professionals and 12 educators) were interviewed over a period of three months. All participants were reported that they experienced at least one adverse reaction/effect. The range of COVID-19 vaccine adverse reactions/effects were one to three adverse effects Pain at the vaccine injection site reported by 49 out of 49 participants; swelling/redness of the vaccine injection site reported by 45 out of 49; fatigue reported by 19 out of 49; headache reported by eight; fever reported by one participant. Majority of the reported adverse reactions were described as moderate or mild and the duration was between one day and one week.
Quantitative and Ultrasensitive In-situ Immunoassay Technology for SARS-CoV-2 Detection in Saliva
Authors: Lee Luke , Liu Fei , Chen Yuchao , Roma Gianluca 
Published: January 2021

Abstract
The COVID-19 pandemic has become an immense global health crisis. However, the lack of efficient and sensitive on-site testing methods limits early detection for timely isolation and intervention. Here, we present a Quantitative and Ultrasensitive in-situ Immunoassay Technology for SARS-CoV-2 detection in saliva (QUIT SARS-CoV-2). Our nanoporous membrane resonator generates a rapid oscillating flow to purify and concentrate SARS-CoV-2 virus in saliva by 40 folds for in-situ detection of viral antigens based on chemiluminescent immunoassay within 20 min. This method achieved a detection sensitivity below 10 0 copies/mL viral load, comparable to the bench-top PCR equipment. The portable QUIT SARS-CoV-2 system, allowing rapid and accurate on-site viral screen with high-throughput sample pooling strategy, can be performed at the primary care settings and substantially improve the detection and prevention of COVID-19.
Bio-fabrication and experimental validation of an Mg – 25Ca – 5Zn alloy proposed for a porous metallic scaffold.
Authors: Becerra Luis Humberto Campos , Rodríguez Marco Antonio Loudovic Hernández , de León Jonathan Puente , Arroyo Raúl Lesso , S Hugo Esquivel , Pérez Eduardo Núñez , Castro Alejandro Torres 
Published: January 2021

Abstract
Background The bio-fabrication of a porous scaffold from a selection procedure of elements taking into account the biological behavior, using Magnesium (Mg) alloyed with Calcium (Ca) and Zinc (Zn), it could work as a treatment for specific pathologies in trauma and oncology, on the one hand, in osteosynthesis, contributing to osseointegration and contributing to infection control through the release of drugs, additionally, another possible attribute of this alloy would be to be used as a complementary treatment for osteosarcoma, this is due to the basification produced by oxidative degradation. (Attack on cancer cells)(1)(2)(3)(4).Methods The evaluation of cell viability of an alloy of Mg − 25 wt% Ca + 5 wt% Zn will strengthen current perspectives on the use of Mg in the clinical evaluation of various treatments in trauma and oncology. Considerations on the preparation of an alloy of Mg − 25 wt% Ca + 5 wt% Zn and its morphological characterization, will help to understand the applicability for the development of new surgical techniques and lead to a deeper investigation in the conception of alternative treatments. However, it is very important to bear in mind the mechanical effect of elements such as Ca and Zn, on the degradation of the alloy matrix, the best alternative to predict the biological - mechanical potential starts with the selection of the essential - nutritional elements (5)(6)(7) and its mechanical evaluation by micro-indentation due to the fragility of the matrix (8)(9).Results Therefore, the morphological evaluation of the specimens of Mg − 25 wt% Ca + 5 wt% Zn will show the crystallinity of the alloy; these results together generate a contribution regarding the design of biomedical alloys for use in treatments for various medical specialties.Conclusions The results indicated that cell viability is not affected, and there are no morphological changes in the cells.
Characterization of Highly Reductive Modification of Tetracycline D-Ring Reveals Enzymatic Conversion of Enone to Alkane
Authors: Nie Qiu-Yue , Ji Zhen-Yu , Hu Yu , Tang Gong-Li 
Published: January 2021

Abstract
Tetracyclines are an eminent family of type II polyketides which possess a variety of decoration on the skeletons. However, apart from the oxidative modification in aureolic acid compounds, there are few cases on the further conversion of α, β-unsaturated ketones in the tetracycline D-ring. Here, we identified two reductases (TjhO5 and TjhD4) that highly reduced the α,β-unsaturated ketone of D-ring in the biosynthesis of unconventional tetracyclines. By identifying related intermediates and conducting isotope incorporation experiments, we demonstrated that the entire transformation could be accomplished by TjhO5 and TjhD4 collectively via two distinct pathways involving different enzymatic mechanisms. A distinctive deoxygenation mechanism was possibly involved in the TjhO5-mediated continuous reduction of C = O to CH2. These findings highlight the unprecedent post-modification of tetracyclines and facilitate further engineering to enrich the structural diversities.
Revisiting the Gastrin-releasing Peptide/bombesin System: A Reverse-evolutionary Study Considering Xenopus
Authors: Hirooka Asuka , Hamada Mayuko , Fujiyama Daiki , Takanami Keiko , Kobayashi Yasuhisa , Oti Takumi , Katayama Yukitoshi , Sakamoto Tatsuya , Sakamoto Hirotaka 
Published: January 2021

Abstract
Bombesin is a putative antibacterial peptide isolated from the skin of the frog, Bombina bombina. Two related (bombesin-like) peptides, gastrin-releasing peptide (GRP) and neuromedin B (NMB) have been found in mammals. The history of GRP/bombesin discovery has caused little attention to be paid to the evolutionary relationship of GRP/bombesin and their receptors in vertebrates. We have classified the peptides and their receptors from the phylogenetic viewpoint using a newly established genetic database and bioinformatics. Here we show, by using a clawed frog (Xenopus tropicalis), that GRP is not a mammalian counterpart of bombesin and also that, whereas the GRP system is widely conserved among vertebrates, the NMB/bombesin system has diversified in certain lineages, in particular in frog species. To understand its derivation of GRP system in the ancestor of mammals, we have focused on the GRP system in Xenopus. Gene expression analyses combined with immunohistochemistry and Western blotting experiments demonstrated that GRP peptides and their receptors are distributed in the brain and stomach of Xenopus. We conclude that GRP peptides and their receptors have evolved from ancestral (GRP-like peptide) homologues to play multiple roles in both the gut and the brain as one of the ‘gut-brain peptide’ systems.
Reduced Vestibular Perception Thresholds in Persistent Postural-Perceptual Dizziness- A Cross-Sectional Study
Authors: Wurthmann Sebastian , Holle-Lee Dagny , Obermann Mark , Roesner Miriam , Nsaka Michael , Scheffler Armin , Kleinschnitz Christoph , Naegel Steffen 
Published: January 2021

Abstract
BACKGROUND: Persistent postural-perceptual dizziness (PPPD) is the most common functional vestibular disorder which is maintained by an interaction of psychological and physiological factors. PPPD patients develop inappropriate postural control strategies characterized by increased self-motion perception. Increased cortical and subcortical excitability may play a role in the pathophysiology of PPPD. We hypothesized that decreased motion perception thresholds reflect one mechanism of the abnormal vestibular responsiveness in this disorder. We investigated the vestibular perception thresholds and the vestibular ocular reflex with a rotatory chair experiment to gain insights in the processing and adaption to vestibular provocation. METHODS: In this cross-sectional study 26 female PPPD patients and 33 healthy female age and matched controls (HC) were investigated sitting in a motorized rotary chair shielded regarding visual and acoustic stimuli. The chair was rotated for 20 minutes with slowly increasing velocity to a maximum of 72°/s. We functionally tested motion perception thresholds and vegetative responses to rotation as well as vestibular-ocular reflex thresholds. We additionally investigated several psychological comorbidities (i.e. depression, anxiety, somatosensory amplification) using validated scores. Conventional dizziness scores were obtained to quantify the experienced dizziness and impact on daily life. RESULTS: PPPD patients showed a significant reduced vestibulo-perceptual threshold (PPPD: 10.9°/s vs. HC: 29.5°/s; p<0.001) with increased motion sensitivity and concomitant vegetative response during and after the chair rotation compared to healthy controls. The extent of increased vestibular sensitivity was in correlation with the duration of the disease (p=0.043). No significant difference was measured regarding nystagmus parameters between both groups.CONCLUSION: PPPD patients showed increased vegetative response as well as decreased vestibulo-perceptual thresholds which are related to disease duration. This is of interest as PPPD might be sustained by increased vestibular excitability leading to motion intolerance and induction of dizziness when exposed to movement.
Ultralow thermal conductivity via topological network control of vibrational localization in amorphous chalcogenides
Authors: Aryana Kiumars , Stewart Derek , Gaskins John , Nag Joyeeta , Read John , Olson David , Grobis Michael , Hopkins Patrick 
Published: January 2021

Abstract
Amorphous chalcogenide alloys are key materials for data storage and energy scavenging applications due to their large non-linearities in optical and electrical properties as well as low vibrational thermal conductivities. Here, we report on a mechanism to suppress the thermal transport in a representative amorphous chalcogenide system, silicon telluride (SiTe), by nearly an order of magnitude via systematically tailoring the cross-linking network among the atoms. As such, we experimentally demonstrate that in fully dense amorphous SiTe the thermal conductivity can be reduced to as low as 0.1 ± 0.01 W/m/K for high tellurium content with a density nearly twice that of amorphous silicon. Using ab-initio simulations integrated with lattice dynamics, we attribute the ultralow thermal conductivity of SiTe to the suppressed contribution of extended modes of vibration, namely propagons and diffusons. This leads to a large shift in the mobility edge - a factor of five - towards lower frequency and localization of nearly 42% of the modes. This localization is the result of reductions in coordination number and a transition from over-constrained to underconstrained atomic network.
A Genome-Wide Association Study Identifies a Novel Susceptibility Locus at The DLGAP1 Gene For Resistant Hypertension in The Japanese Population
Authors: Takahashi Yasuo , Yamazaki Keiko , Kamatani Yoichiro , Kubo Michiaki , Matsuda Koichi , Asai Satoshi 
Published: January 2021

Abstract
Numerous genetic variants associated with hypertension and blood pressure are known, but there is a paucity of evidence from genetic studies of resistant hypertension, especially in the Asian populations. To identify novel genetic loci associated with resistant hypertension in the Japanese population, we conducted a genome-wide association study with 2,705 resistant hypertension cases and 21,296 mild hypertension controls, all from BioBank Japan. We identified one novel susceptibility locus, rs1442386 on chromosome 18p11.3 (DLGAP1), achieving genome-wide significance (odds ratio (95% CI) = 0.85 (0.81–0.90), P = 3.75 × 10−8) and 17 loci showing suggestive association, including rs62525059 of 8q24.3 (CYP11B2). We further detected biological processes associated with resistant hypertension, including chemical synaptic transmission, regulation of transmembrane transport, neuron development and neurological system processes, highlighting the importance of the nervous system. This study provides insights into the etiology of resistant hypertension in the Japanese population.
Management of Isoniazid Preventive Therapy in Southern Lima, Peru: An Analysis of Health Center Characteristics
Authors: Zegarra-Chapoñan Roberto , Bonadonna Lily Victoria , Yuen Courtney M. , Martina-Chávez Martha Brigida , Zeladita Jhon 
Published: January 2021

Abstract
Background: Prevention of TB through the use of preventive treatment is a critical activity in the elimination of TB. Health personnel are a key element in achieving proper management of isoniazid preventive therapy (IPT) for TB contacts. This study aims to determine the association between health center characteristics and indicators of TB contact care management with isoniazid preventive therapy in southern Lima. Methods: We conducted an ecological study. Through the review of medical records, we identified children and adolescent contacts of tuberculosis patients, who initiated IPT between 2016 and 2018. We assessed bivariate associations between clinic staffing and IPT initiation and completion using binomial logistic regression with robust standard errors.Results: We included 977 contacts, among whom 69% took more than a week to start IPT and 41% did not complete IPT. For those who did successfully complete IPT, 58% were not medically evaluated with three follow-up appointments. While completion of IPT and 3 clinical appointments was better among health centers with more physicians and nurses, these differences were not statistically significant. Contact sex was associated with initiating IPT (p=0.005), and contact age was associated with completion of IPT (p=0.025) and completion of clinical evaluations (p=0.041). Conclusion: There are significant gaps in IPT management in health centers of southern Lima, Peru, but insufficient staffing of health centers may not be responsible. Further research is needed to identify how IPT management can be improved, including through improving training and staffing.
The Expression and Prognostic Values of Epidermal Growth Factor Receptor Family in Glioma
Authors: xu bin , ji wei , huo zhengyuan , bian zheng , jiao jiantong , sun jun , shao junfei 
Published: January 2021

Abstract
Background: Epidermal growth factor receptor (EGFR) family belongs to the transmembrane protein receptor of tyrosine kinase I subfamily, including 4 members, they are EGFR/ERBB1, ERBB2, ERBB3, ERBB4. The EGFR family is closely related to the occurrence and development of a variety of cancers.Material/Methods: In this research, we used multiple online bioinformatics websites including ONCOMINE, TCGA, CGGA, TIMER, cBioPortal, GeneMANIA and DAVID to study the expression profiles, prognostic values and immune infiltration correlations of EGFR family in glioma.Results: We found that EGFR and ERBB2 mRNA expression levels were the higher in glioblastoma (GBM, WHO IV) than other grades (WHO grade II&III), While ERBB3 and ERBB4 mRNA expression levels were opposite. EGFR and ERBB2 were notably downregulated in IDH mutant gliomas, while ERBB3 and ERBB4 were the opposite. Besides, ERBB2 and ERBB4 in glioma patients were associated with poor prognosis. In addition, correlation analysis between EGFR family expression and immune infiltrating levels in glioma showed that EGFR family expression and immune infiltrating levels were significantly correlated. PPI network of EGFR family in glioma and enrichment analysis showed that EGFR family and their interactors mainly participated in regulation of cell motility involved integrin receptors and Rho family GTPases.Conclusions: In summary, this study indicates that EGFR family may become potential targets and new prognostic markers for glioma.
SNX17 regulates antigen internalization and phagosomal maturation by dendritic cells
Authors: Cebrian Ignacio , Dinamarca Sofía , Croce Cristina , Salvioni Anna , Garrido Facundo , Fidalgo Sandra Estrada , Mayorga Luis , Blanchard Nicolas 
Published: January 2021

Abstract
Cross-presentation is the process whereby antigenic peptides derived from exogenous antigens are associated to MHC class I molecules triggering the activation of CD8+ T lymphocytes. The endocytic route of dendritic cells (DCs) is strongly specialized to achieve antigen cross-presentation efficiently, which is crucial to initiate cytotoxic immune responses against many pathogens (i.e. Toxoplasma gondii) and tumors. Nevertheless, the endosomal molecular effectors involved in this process are not completely understood. In particular, the role of sorting nexin (SNX) proteins in cross-presentation has never been addressed. In this work, we identify the endosomal protein SNX17 as a key regulator of antigen internalization and cross-presentation by DCs. Our results demonstrate that SNX17 expression in DCs is essential to guarantee a normal cross-presentation of soluble, particulate and T. gondii-associated antigens. The silencing of SNX17 expression in DCs significantly affected the uptake of exogenous antigens by fluid-phase endocytosis and phagocytosis, but not by receptor-mediated endocytosis. Moreover, the knock-down of SNX17 impaired T. gondii invasion, CD11b integrin recycling and hampered the organization of the actin cytoskeleton. Finally, we show that SNX17 controls the proper maturation of DC phagosomes. Our findings provide compelling evidence that SNX17 plays a central role in the modulation of DC endocytic network, which is crucial for competent antigen internalization and cross-presentation.
NEUROD1 Mutation in an Italian Patient With Maturity Onset Diabetes of the Young 6: a Case Report
Authors: Brodosi Lucia , Baracco Bianca , Mantovani Vilma , Pironi Loris 
Published: January 2021

Abstract
Background and Aims: maturity onset diabetes of the young (MODY) is a monogenic, autosomal, dominant disease characterized by a single genetic mutation that results in beta-cells disfunction with consequent hyperglycemia. It represents a rare form of diabetes (1-2% of all the cases). Sulphonylureas (SU) represent the first line treatment for this form of diabetic disease. NEUROD1 is a transcription factor expressed by pancreatic and nervous tissues that is necessary for a proper development of beta cells. A mutation of NEUROD1 gene has been found to cause beta-cells dysfunction, inadequate insulin secretion, and hyperglycemia (MODY 6). A recent case report has documented for the first time a new missense mutation (p.Met114Leu c.340A> C), of the NEUROD1 gene pathogenetic for diabetes mellitus.Methods and Results: We report the case of a 50 years-old man who presented the same mutation, and who was able to suspend rapid insulin after the diagnosis and treatment with SU. Interestingly, our patient had an early onset dilated cardiomyopathy but no other data about cardiac diseases in patients with MODY 6 are available.Conclusions: Diagnostic criteria for MODY can overlap with other kinds of diabetes and most cases are still misdiagnosed as diabetes type 1 or 2. The disease should be suspected in patients with a strong family history of diabetes, normal BMI, early onset and no autoimmunity.
Optical Coherence Tomography Angiography Findings in Non-Infectious Posterior Uveitis: A Controlled Study
Authors: Sizmaz Selcuk , Esen Ebru , Isik-Ericek Puren , Demircan Nihal 
Published: January 2021

Abstract
Background: To analyze the microvascular parameters using optical coherence tomography angiography (OCTA) in non-infectious posterior uveitis (PU) patients. Methods: In this cross-sectional study, OCTA images of patients with non-infectious PU were evaluated. The vessel densities (VD) in the superficial and deep capillary plexuses (SCP & DCP), foveal avascular zone (FAZ) area were measured and compared to healthy controls. Results: The study cohort comprised 64 patients with age and gender-matched groups. The VD in the SCP was 43.9±3.9% in the whole image; 18.4±4.8% in the fovea, 43.9±4.6% in the parafoveal area, and 43.4±9.7% in the perifoveal zone, in eyes with uveitis. These were respectively 48.8±2.9%, 22.9±6.9%, 50.9±3.0%, and 49.2±3.1% in the control group (p=0.0001, 0.043, 0.0001, 0.01, respectively). The changes in the DCP in eyes with uveitis were not significant. In the uveitis group, the FAZ was significantly enlarged compared to controls (0.37±0.1 microns vs. 0.24±0.2 microns, p=0.046). Conclusion: OCTA depicted significant changes including decreased VD in the SCP and enlarged FAZ in non-infectious PU.
Acupoint Catgut Embedding versus Acupuncture for Simple Obesity A Systematic Review and Meta-Analysis of Randomized Controlled Trials
Authors: Wei Jiali , Lai Lily , Lin Zhechao , Liu Jianping , Han Mei 
Published: January 2021

Abstract
Background: Acupuncture is very popular for reducing weight in China, in this work, we evaluate the clinical effectiveness and safety of acupoint catgut embedding and acupuncture on simple obesity by Meta-analysis.Methods: Studies on clinical randomized controlled trials of acupoint catgut embedding for simple obesity which were published from January 2015 to November 2020 were searched in PubMed, VIP, CNKI and Wanfang databases. And those that met the inclusion criteria were screened. RevMan5.3 was used for Meta-analysis of effectiveness rate, BMI, waist circumference and body mass. The “Risk of Bias” tool was used to evaluate the quality of included studies. R studio software was used for the measurement of publication bias.Results: A total of 33 studies were included for meta-analysis, including 2,685 patients with simple obesity. Meta-analysis results showed the comparison of effectiveness rate was RR= 1.12, 95%CI [1.08,1.16], the comparison of BMI was MD=-1.11, 95%CI [-2.04, -0.18], the comparison of waist circumference was MD=-2.27, 95%CI [-4.26,-0.28], and the comparison of body mass was MD=-2.24,95%CI [-3.78,-0.71].On the basis of diet and exercise intervention, the effectiveness rate RR=2.11, 95%CI [1.38,3.24], BMI MD=-0.88, 95%CI [-1.35,-0.40], waist circumference MD=-1.10, 95%CI [-4.27,2.07]and body mass MD=-0.68, 95%CI [-2.90,1.54].The risk of bias of included literatures was low.Conclusions: Acupoint catgut embedding therapy was slightly better than acupuncture therapy in effectiveness rate, BMI, waist circumference and body mass. Moreover, the treatment frequency of acupoint catgut embedding is less with larger stimulation intensity, which is more conducive to clinical promotion.