bluuebunny/crossref_metadata_2025_split huggingface dataset:
High-throughput analysis of B3GLCT regulation predicts phenotype of Peters’ Plus Syndrome in line with the miRNA Proxy Hypothesis
Authors: Thu Chu T. , Chung Jonathan Y. , Dhawan Deepika , Vaiana Christopher A. , Mahal Lara K. 
Published: April 2021

ABSTRACTMicroRNAs (miRNAs, miRs) finely tune protein expression and target networks of 100s-1000s of genes that control specific biological processes. They are critical regulators of glycosylation, one of the most diverse and abundant posttranslational modifications. In recent work, miRs have been shown to predict the biological functions of glycosylation enzymes, leading to the “miRNA proxy hypothesis” which states, “if a miR drives a specific biological phenotype…, the targets of that miR will drive the same biological phenotype.” Testing of this powerful hypothesis is hampered by our lack of knowledge about miR targets. Target prediction suffers from low accuracy and a high false prediction rate. Herein, we develop a high-throughput experimental platform to analyze miR:target interactions, miRFluR. We utilize this system to analyze the interactions of the entire human miRome with beta-3-glucosyltransferase (B3GLCT), a glycosylation enzyme whose loss underpins the congenital disorder Peters’ Plus Syndrome. Although this enzyme is predicted by multiple algorithms to be highly targeted by miRs, we identify only 27 miRs that downregulate B3GLCT, a >96% false positive rate for prediction. Functional enrichment analysis of these validated miRs predict phenotypes associated with Peters’ Plus Syndrome, although B3GLCT is not in their known target network. Thus, biological phenotypes driven by B3GLCT may be driven by the target networks of miRs that regulate this enzyme, providing additional evidence for the miRNA Proxy Hypothesis.
Noradrenergic alpha-2A receptor activation suppresses courtship vocalization in male Japanese quail
Authors: Tobari Yasuko , Masuzawa Ami , Harada Norika , Suzuki Kenta , Meddle Simone L. 
Published: April 2021

ABSTRACTMale Japanese quail produce high-frequency crow vocalizations to attract females during the breeding season. The nucleus of intercollicularis (ICo) is the midbrain vocal center in birds and electrical stimulation of the ICo produces calls that include crowing. Noradrenaline plays a significant role in sexual behavior but the contribution of noradrenaline in the control of courtship vocalizations in quail has not been well established. Using dose-dependent intracerebroventricular injection of clonidine, an α2-adrenergic receptor- specific agonist, crowing vocalization was immediately suppressed. At the same time as crow suppression by clonidine there was a reduction of immediate early gene, zenk mRNA, in the ICo; no zenk mRNA expression was detected in the dorsomedial division of the nucleus. Using histochemistry, we determined that the ICo receives noradrenergic innervation and expresses α2A-adrenergic receptor mRNA. Taken together, these data suggest that noradrenaline regulates courtship vocalization in quail, possibly via the alpha2A- adrenergic receptor expressed on ICo neurons.
Title: Multi-Scale LM/EM Neuronal Imaging from Brain to Synapse with a Tissue Clearing Method, ScaleSF
Authors: Furuta Takahiro , Yamauchi Kenta , Okamoto Shinichiro , Takahashi Megumu , Kakuta Soichiro , Ishida Yoko , Takenaka Aya , Yoshida Atsushi , Uchiyama Yasuo , Koike Masato , Isa Kaoru , Isa Tadashi , Hioki Hiroyuki 
Published: April 2021

AbstractThe mammalian brain is organized over sizes that span several orders of magnitude, from synapses to the entire brain. Thus, a technique to visualize neural circuits across multiple spatial scales (multi-scale neuronal imaging) is vital for deciphering brain-wide connectivity. Here, we developed this technique by coupling successive light microscope/electron microscope (LM/EM) imaging with an ultrastructurally-preserved tissue clearing method, ScaleSF. Our multi-scale neuronal imaging incorporates 1) brain-wide macroscopic observation, 2) mesoscopic circuit mapping, 3) microscopic subcellular imaging, and 4) EM imaging of nanoscopic structures, allowing seamless integration of structural information from the brain to synapses. We applied the technique to three neural circuits of two different species, mouse striatofugal, mouse callosal, and marmoset corticostriatal projection systems, and succeeded in the simultaneous interrogation of their circuit structure and synaptic connectivity in a targeted way. Our multi-scale neuronal imaging will significantly advance the understanding of brain-wide connectivity by expanding the scales of objects.
A prediction: granule cells can perform linearly non-separable computations
Authors: Cazé Romain D. 
Published: April 2021

AbstractMultiple studies show how dendrites might extend some neurons’ computational capacity. Past works most often focus on pyramidal cells which have an extended dendritic tree where voltage can vary independently; pyramidal neurons’ dendrites emit local spikes turning dendritic branches into local non-linear subunits. However, these studies leave a large fraction of the nervous system unexplored. Here, we study a neuron with a modest dendritic and non-spiking dendrites. Granule cells’ dendrites do not spike and these cells’ membrane voltage remain constant over the neuron. This study uses a conjunction of Boolean algebra and biophysical modelling to predict that Granule cells can perform linearly non-separable computations. In a previous study, we found a linearly non-separable Boolean function possible to implement without dendritic spikes, we coined this computation the feature binding problem. In the present work, we use the intrinsic saturation of synaptic conductance to implement this computation in a biophysical model. An integrate and fire, cannot perform such type of computations. Confirming our prediction would change how we understand the nervous system.
Mechanism of transcription modulation by the transcription-repair coupling factor
Authors: Paudel Bishnu P , Xu Zhi-Qiang , Jergic Slobodan , Oakley Aaron J , Sharma Nischal , Brown Simon HJ , Bouwer James C , Lewis Peter J , Dixon Nicholas E , van Oijen Antoine M , Ghodke Harshad 
Published: April 2021

ABSTRACTElongation by RNA polymerase is dynamically modulated by accessory factors. The transcription-repair coupling factor (TRCF) recognizes distressed RNAPs and either rescues transcription or initiates transcription termination. Precisely how TRCFs choose to execute either outcome remains unclear. WithEscherichia colias a model, we used single-molecule assays to study dynamic modulation of elongation by Mfd, the bacterial TRCF. We found that nucleotide-bound Mfd converts the elongation complex (EC) into a catalytically poised state, presenting the EC with an opportunity to restart transcription. After long-lived residence in this catalytically poised state, ATP hydrolysis by Mfd remodels the EC through an irreversible process leading to loss of the RNA transcript. Further, biophysical studies revealed that the motor domain of Mfd binds and partially melts DNA containing a template strand overhang. The results explain pathway choice determining the fate of the EC and provide a molecular mechanism for transcription modulation by TRCF.
Can business model innovation help SMEs in the food and beverage industry to respond to crises? Findings from a Swiss brewery during COVID-19
Authors: Bivona Enzo , Cruz Margarita 
Published: April 2021

PurposeThe purpose of this paper is to show how different business model innovations (BMIs) help small and medium-sized enterprises (SMEs) in the food and beverage industry to navigate turbulent and uncertain environments such as the coronavirus economic crisis (COVID-19).Design/methodology/approachThe paper adopts an in-depth case study approach and uses a dynamic business modeling (DBM) approach to analyze how a pioneer craft brewery in Switzerland implemented innovative actions undertaken during the COVID-19 pandemic.FindingsThe paper offers a novel framework describing three processes helping SMEs to implement innovations in their business model (BM) to respond in an effective way to crises, such as the COVID-19 pandemic. The first process refers to SMEs' ability to leverage readily available resources and allows SMEs to rapidly use their current knowledge to react to the changing environment amid the crisis. The second process points at SMEs' ability to transform existing resources into novel products or solutions. Finally, by mobilizing distant resources from their network, SMEs can obtain new resources and knowledge that facilitate the implementation of major changes in their BM.Originality/valueUnlike previous studies, this research adopts a cause-and-effect perspective to make explicit how SMEs' BM changes affect strategic resources, key drivers and processes, thereby impacting performance. The analysis of the multiple reinforcing and balancing feedback loops resulting from the DBM approach can help SME entrepreneurs learn how and what changes are required in their BM to effectively face turbulent times, such as the COVID-19 crisis. From such an analysis, it emerged that the ability of SMEs to effectively implement innovations amid a crisis depends in large part on their collaborations with business partners and their ability to use and transform internal and external knowledge. In addition, as the future evolution of the COVID-19 crisis is still ongoing and uncertain, this study offers a unique perspective for SMEs in the food and beverage industry as the situation unfolds rather than after the fact.
Perceived stress and psychological well-being of working mothers during COVID-19: a mediated moderated roles of teleworking and resilience
Authors: Kapoor Vartika , Yadav Jaya , Bajpai Lata , Srivastava Shalini 
Published: April 2021

PurposeThe present study examines the mediating role of teleworking and the moderating role of resilience in explaining the relationship between perceived stress and psychological well-being of working mothers in India. Conservation of resource theory (COR) is taken to support the present study.Design/methodology/approachThe data of 326 respondents has been collected from working mothers in various sectors of Delhi NCR region of India. Confirmatory factor analysis was used for construct validity, and SPSS Macro Process (Hayes) was used for testing the hypotheses.FindingsThe results of the study found an inverse association between perceived stress and psychological well-being. Teleworking acted as a partial mediator and resilience proved to be a significant moderator for teleworking-well-being relationship.Research limitations/implicationsThe study is based at Delhi NCR of India, and future studies may be based on a diverse population within the country to generalize the findings in different cultural and industrial contexts. The present work is based only on the psychological well-being of the working mothers, it can be extended to study the organizational stress for both the genders and other demographic variables.Practical implicationsThe study extends the research on perceived stress and teleworking by empirically testing the association between perceived stress and psychological well-being in the presence of teleworking as a mediating variable. The findings suggest some practical implications for HR managers and OD Practitioners. The organizations must develop a plan to support working mothers by providing flexible working hours and arranging online stress management programs for them.Originality/valueAlthough teleworking is studied previously, there is a scarcity of research examining the impact of teleworking on psychological well-being of working mothers in Asian context. It would help in understanding the process that how teleworking has been stressful for working mothers and also deliberate the role of resilience in the relationship between teleworking and psychological well-being due to perceived stress, as it seems a ray of hope in new normal work situations.
On the link between policy uncertainty and housing permits: asymmetric evidence from state-level data in the USA
Authors: Bahaman-Oskooee Mohsen , Ghodsi Hesam , Hadzic Muris 
Published: April 2021

Purpose
The purpose of this study is to assess the symmetric and asymmetric impact of a measure of policy uncertainty on house permits issued in each state of the USA.

Design/methodology/approach
To assess the symmetric effects, the authors use Pesaran et al.’s (2001) linear autoregressive distributed lag (ARDL) approach to error-correction modeling. To assess the asymmetric effects, they rely upon Shin et al.’s (2014) nonlinear ARDL approach to error-correction modeling. Both approaches have the advantage of producing short-run and long-run effects in one step.

Findings
The authors find short-run symmetric effects of policy uncertainty on house permits issued in 22 states that lasted into the long run in three states only. However, the numbers were much higher when they estimated the possibility of asymmetric effects of policy uncertainty. Indeed, they found short-run asymmetric effects in 38 states and long-run asymmetric effects in 18 states.

Originality/value
Some previous studies assessed the effects of a measure of policy uncertainty on house prices. In this paper, the authors extend the same analysis to the supply side of the housing market by assessing the effects of policy uncertainty on house permits in each state of the USA.
A hedonic assessment of the relative importance of structural, locational and neighbourhood factors on advertised rents in Malta
Authors: Micallef Brian , Ellul Reuben , Debono Nathaniel 
Published: April 2021

Purpose
The private rental market in Malta has expanded significantly in recent years, but as at 2020, no official rent index is yet published. This paper aims to construct such an index and explores the relative importance of structural, locational and neighbourhood factors to advertised rents.

Design/methodology/approach
The authors compile hedonic indices for advertised rents in Malta collected from publicly available sources using webscraping techniques. The database comprises more than 25,000 listings with information on various property attributes. Hedonic regressions are estimated using ordinary least squares and rent indices are computed using three alternative methods: the time dummy method, the rolling time dummy method and the average characteristics method. For the latter, indices are computed using the Laspeyres, Paasche and Fisher methods.

Findings
The results from the hedonic indices indicate that the annual growth rate in advertised rents was slowing down during 2019, albeit still remaining relatively high, while in 2020, advertised rents contracted sharply, amplified by the effects of COVID-19. The findings also reveal that advertised rental prices are significantly influenced by various structural, locational and neighbourhood factors.

Originality/value
This paper introduces the first rent index in Malta that will be used to monitor developments in the rental segment of the housing market and for financial stability purposes given the share of buy-to-let properties. It also provides various elasticities on the impact of property attributes on advertised rents in Malta. Finally, the study contributes to the literature on the effect of foreign-born residents on advertised rents.
Effect of background music and hierarchy-of-effects in watching women's running shoes advertisements
Authors: Uhm Jun-Phil , Lee Hyun-Woo , Han Jin-Wook , Kim Dong-Kyu 
Published: April 2021

PurposeThe purpose of this study is to examine the effect of background music on consumer's psychological and physiological responses when watching sports advertisements. We investigated how consumers' exposure to background music affects emotional arousal, attention, brand attitude and purchase intentions; and further tested consumers' information processing by using the same measures. Effects of music on viewer responses were hypothesized using arousal theory while the information processing was hypothesized using hierarchy-of-effects model.Design/methodology/approachWe employed a between-subjects experimental design with random assignment. Fifty-four participants were recruited with 27 in an experimental group and 27 in a control group. Quantitative electroencephalogram (qEEG) and self-report measures were used to assess information processing. A multivariate analysis of covariance was conducted to compare the mean differences of variables between the groups. Partial least squares algorithm and bootstrapping were performed to further explore the relationships among the measures.FindingsMean differences indicated that the background music exposed group's emotional arousal, attention, brand attitude and purchase intention were significantly higher than those of the non-exposure group. Path analysis showed that the level of arousal induced by watching sports advertisements affected attention, attention affected brand attitude and brand attitude affected purchase intention. Indirect paths from arousal to brand attitude and attention to purchase intention were significant.Originality/valueThis study provides practical implications for sports marketers regarding methods to increase the effectiveness of sport advertisement. Results might contribute theoretically to the sports advertisement field by demonstrating the relationship between physiological and marketing-effect factors. Our method of measuring physiological response using qEEG is also expected to influence physiological measurement in sports marketing.
Identifying the future direction of legal deposit in the United Kingdom: The Digital Library Futures approach
Authors: Gooding Paul , Terras Melissa , Berube Linda 
Published: April 2021

PurposeTo date, there has been little research into users of the Legal Deposit Libraries (Non-Print Works) Regulations 2013. This paper addresses that gap by presenting key findings from the AHRC-funded Digital Library Futures project. Its purpose is to present a “user-centric” perspective on the potential future impact of the digital collections that are being created under electronic legal deposit regulations.Design/methodology/approachThe study utilises a mixed methods case study of two academic legal deposit libraries in the United Kingdom: The Bodleian Libraries, University of Oxford; and Cambridge University Library. It combines surveys of users, web log analysis and expert interviews with librarians and cognate professionals.FindingsUser perspectives on NPLD were not fully considered in the planning and implementation of the 2013 regulations. The authors present findings from their user survey to show how contemporary tensions between user behaviour and access protocols risk limiting the instrumental value of NPLD collections, which have high perceived legacy value.Originality/valueThis is the first study to address the user context for UK Non-Print Legal Deposit. Its value lies in presenting a research-led user assessment of NPLD and in proposing “user-centric” analysis as an addition to the existing “four pillars” of legal deposit research.
The impact of the Vietnam Bank for Social Policies preferential credit on household welfare in Vietnam: a panel data analysis
Authors: Duong An , Antriyandarti Ernoiz 
Published: April 2021

PurposeThe study examines the impact of the preferential credit provided by the Vietnam Bank for Social Policies on poverty reduction in Ninh Binh province, Vietnam. It also identifies and ranks the barriers of accessing the credit.Design/methodology/approachThe study applies fixed-effects method to handle the panel data to examine the impact of the credit on poverty reduction. It also uses face-to-face interviews and group discussions to identify and rank the barriers of accessing to the credit.FindingsThe results show that the credit (represented by loan volume) positively and significantly helps improve household income, but does not help to improve household consumption. The major barriers include the time spent to get to the nearest bank branch, banking support services provided to clients and the transparency of household poverty status assessment.Research limitations/implicationsData are collected in three years and the number of the observations is limited to 300 households.Practical implicationsThe VBSP preferential credit may need to be modified to significantly help reduce poverty and the VBSP and involved parties may need to eliminate the barriers so that the poor can have a better access to the credit.Social implicationsThe VBSP preferential credit is one of the reasonable sources that can help eliminate poverty though increasing household income.Originality/valueThe VBSP preferential credit can help increase household income, but does not really help improve household consumption due to the small volume of loans. In addition, banking support services and the household poverty assessment are seen as barriers to the access of the poor.
Opportunity zones: do tax benefits go to the most distressed communities?
Authors: Barth James R. , Sun Yanfei , Zhang Shen 
Published: April 2021

Purpose
The exact criteria used by state governors for choosing opportunity zones (OZs) are not publicly available. This paper aims to examine whether state governors selected the most distressed communities, or those with the highest proportions of minorities, as OZs.

Design/methodology/approach
This paper compares the distressed communities chosen as OZs in states throughout the country to an equal number of those eligible distressed communities but not selected. Moreover, this paper uses regression analysis to determine whether the poverty rate, median family income, population, percentage of population that is minority and the percentage of population that is African American are significant explanatory factors in the choice of OZs.

Findings
After describing the tax incentives for investing in OZs, this paper documents that governors did not select many of the most distressed communities, or those with high proportions of minorities, in their individual states.

Originality/value
This paper describes in some detail the way in which investors may generate tax benefits by investing in eligible property or businesses in OZs. It also examines the extent to which the degree of poverty and the percentage of the population that is minority (and African American) were key factors in the selection of OZs. It arises an issue that the chosen communities are not necessarily those most in need of more investment or those heavily populated by minorities, particularly African Americans.