bluuebunny/crossref_metadata_2025_split huggingface dataset:
CAMSAP2 organizes a γ-tubulin-independent microtubule nucleation centre
Authors: Imasaki Tsuyoshi , Kikkawa Satoshi , Niwa Shinsuke , Saijo-Hamano Yumiko , Shigematsu Hideki , Aoyama Kazuhiro , Mitsuoka Kaoru , Aoki Mari , Sakamoto Ayako , Tomabechi Yuri , Sakai Naoki , Shirouzu Mikako , Taguchi Shinya , Yamagishi Yosuke , Setsu Tomiyoshi , Sakihama Yoshiaki , Shimizu Takahiro , Nitta Eriko , Takeichi Masatoshi , Nitta Ryo 
Published: March 2021

AbstractMicrotubules are dynamic polymers consisting of αβ-tubulin heterodimers. The initial polymerization process, called microtubule nucleation, occurs spontaneously via αβ-tubulin. Since a large energy barrier prevents microtubule nucleation in cells, the γ-tubulin ring complex is recruited to the centrosome to overcome the nucleation barrier. However, detachment of a considerable number of microtubules from the centrosome is known to contribute to fundamental processes in cells. Here, we present evidence that minus-end-binding calmodulin-regulated spectrin-associated protein 2 (CAMSAP2) serves as a strong nucleator for microtubule formation from soluble αβ-tubulin independent of γ-tubulin. CAMSAP2 significantly reduces the nucleation barrier close to the critical concentration for microtubule polymerization by stabilizing the longitudinal contacts among αβ-tubulins. CAMSAP2 clusters together with αβ-tubulin to generate nucleation intermediates, from which numerous microtubules radiate, forming aster-like structures. Our findings suggest that CAMSAP2 supports microtubule growth by organizing a nucleation centre as well as by stabilizing microtubule nucleation intermediates.
A novel function for Rab proteins during maturation of secretory granules
Authors: Neuman Sarah D. , Lee Annika R. , Selegue Jane E. , Cavanagh Amy T. , Bashirullah Arash 
Published: March 2021

ABSTRACTRegulated exocytosis is an essential process whereby professional secretory cells synthesize and secrete specific cargo proteins in a stimulus-dependent manner. Cargo-containing secretory granules are synthesized in thetrans-GolgiNetwork (TGN); after budding from the TGN, granules undergo many modifications, including a dramatic increase in size. These changes occur during a poorly understood process called secretory granule maturation. Here we leverage the professional secretory cells of theDrosophilalarval salivary glands as a model system to characterize a novel and unexpected role for Rab GTPases during secretory granule maturation. We find that secretory granules in the larval salivary glands increase in size ~300-fold between biogenesis and release, and loss ofRab1orRab11dramatically reduces granule size. Surprisingly, we find that Rab1 and Rab11 protein localize to secretory granule membranes. Rab11 associates with granule membranes throughout the maturation process, and Rab11 is required for recruitment of Rab1. In turn, Rab1 associates specifically with immature secretory granules and drives granule growth. In addition to their roles in granule growth, bothRab1andRab11appear to have additional roles during exocytosis;Rab11function is necessary for exocytosis, while the presence ofRab1on immature granules may prevent precocious exocytosis. Overall, these results highlight a new and unexpected role for Rab GTPases in secretory granule maturation.
Organizing municipal audit: contracting out and audit costs in Norway
Authors: Johnsen Åge 
Published: March 2021

PurposeThis paper analyses the deregulation of the municipal audit market in Norway, in particular how organization of the service affected audit costs.Design/methodology/approachThe paper uses multiple regression analysis of administrative as well as survey data of organizational structure and audit costs from 312 municipalities in Norway in 2012.FindingsThe introduction of contracting out in the municipal audit market in Norway in 2004 contributed to a reduction in audit fees eight years after the deregulation, compared to the situation four years before the deregulation. The type of audit, mixing inter-municipal co-operation and contracting private auditors, was related to lower costs.Research limitations/implicationsThe data does not include audit quality and are restricted to one country.Practical implicationsThe municipalities that combined inter-municipal co-operation and contracting a private auditor achieved the lowest costs. Hence, neither pure in-house production (“make”) nor outsourcing (“buy”) but mixing several governance forms (hybrid organization) was related to low costs.Social implicationsContracting out is a core element of new public management (NPM) but has often been a contested tool in public policy. This paper provides empirical evidence on the effects of a reform of a professional service, which is relevant for many services in the public sector.Originality/valueThis paper contributes by filling some of the gaps in the public sector accounting and public management reform literature by studying the organization and costs of the municipal audit, specifically by including transaction costs, addressing plural governance forms in addition to pure in-house production, inter-organizational co-operation and market contracting and by studying long-term effects.
Team social media usage and intra-team competition and cooperation: a social information processing perspective
Authors: Zhang Shuqin , Huang Qian , Liu Hefu , Wang Youying 
Published: March 2021

PurposeThis study aims to explore how team task-related social media usage (TSMU) and social-related social media usage (SSMU) affect employees' perceptions of intra-team cooperation and competition and further individual creativity.Design/methodology/approachThis study conducted a questionnaire survey on enterprises in China that have implemented social media and obtained 348 useable questionnaires from 55 work teams.FindingsThe results revealed that employees' perceptions of intra-team cooperation and competition can promote employees' creativity. Employees' cooperation perception can be significantly positively affected by TSMU and SSMU, whereas employees' competition perception can be significantly positively affected by TSMU. Regarding congruence, the results indicated that the more balanced between TSMU and SSMU, the stronger the competition perception.Practical implicationsManagers should pay critical attention to the role of team social media usage (SMU) in shaping employees' perceptions of their team environments. They should realize the different outcomes and the joint effects of the different types of SMU.Originality/valueThis study contributes to the social media literature by explaining the impact of team SMU on employees' perceptions and evaluations of team environments based on the social information processing theory. The study presents the relationships among team SMU, employees' perceptions of cooperation and competition and employee creativity. Moreover, this study expands research on the trade-off of SMU by exploring the impact of balanced and imbalanced SMU in a work team.
Tacrolimus dose, blood concentrations and acute nephrotoxicity, but not CYP3A5/ABCB1 genetics, are associated with allograft tacrolimus concentrations in renal transplant recipients
Authors: Sallustio Benedetta C. , Noll Benjamin D. , Hu Rong , Barratt Daniel T. , Tuke Jonathan , Coller Janet K. , Russ Graeme R. , Somogyi Andrew A. 
Published: March 2021

AimsLong‐term use of the immunosuppressant tacrolimus is limited by nephrotoxicity. Following renal transplantation, the risk of nephrotoxicity may be determined more by allograft than by blood tacrolimus concentrations, and thus may be affected by donor CYP3A5 and ABCB1 genetics. Little is known regarding factors that determine tacrolimus intrarenal exposure.MethodsThis study investigated the relationship between trough blood (C0Blood) and allograft (CGraft) tacrolimus concentrations and tacrolimus dose, haematocrit, genetics, acute nephrotoxicity, rejection status, delayed graft function, and time post‐transplant. C0Blood and CGraft were quantified in 132 renal transplant recipients together with recipient and donor CYP3A5 (rs776746) and ABCB1 3435 (rs1045642) genotypes.ResultsC0Blood ranged from 2.6 to 52.3 ng/mL and CGraft from 33 to 828 pg/mg tissue. Adjusting for dose, recipients who were CYP3A5 expressors had lower C0Blood compared to nonexpressors, whilst delayed graft function was associated with higher C0Blood. Linear regression showed that the significant predictors of CGraft were C0Blood (point‐wise P = 7 × 10−10), dose (P = .004) acute nephrotoxicity (P = .002) and an interaction between C0Blood and acute tacrolimus nephrotoxicity (P = .0002), with an adjusted r2 = 0.35 and no contribution from donor or recipient CYP3A5 or ABCB1 genotype. The association between CGraft and acute nephrotoxicity depended on one very high CGraft (828 pg/mg tissue).ConclusionsRecipient and donor CYP3A5 and ABCB1 3435C>T genotypes are not determinants of allograft tacrolimus exposure in kidney transplant recipients. However, tacrolimus dose and C0Blood were significant predictors of CGraft, and the relationship between C0Blood and CGraft appeared to differ in the presence or absence of acute nephrotoxicity.
Spliced HLA-bound peptides: a Black Swan event in immunology
Authors: Faridi P , Dorvash M , Purcell A W 
Published: March 2021

Summary
Peptides that bind to and are presented on the cell surface by human leucocyte antigen (HLA) molecules play a critical role in adaptive immunity. For a long time it was believed that all the HLA-bound peptides were generated through simple proteolysis of linear sequences of cellular proteins, and therefore are templated in the genome and proteome. However, evidence for untemplated peptide ligands of HLA molecules has accumulated during the last two decades, with a recent global analysis of HLA-bound peptides suggesting that a considerable proportion of HLA-bound peptides are potentially generated through splicing/fusion of discontinuous peptide segments from one or two distinct proteins. In this review, we will evaluate recent discoveries and debates on the contribution of spliced peptides to the HLA class I immunopeptidome, consider biochemical rules for splicing and the potential role of these spliced peptides in immune recognition.
The longitudinal relationship between cannabis use and hypertension
Authors: Haleem Ahmed , Hwang Yoseob Joseph , Elton‐Marshall Tara , Rehm Jürgen , Imtiaz Sameer 
Published: March 2021

AbstractIntroductionThe relationship between cannabis use and hypertension is not clear based on prior epidemiological studies. Thus, we examined this relationship over a 3‐year follow‐up period using a large population‐based sample from the USA.MethodsSelf‐reported longitudinal data were obtained from the National Epidemiologic Survey on Alcohol and Related Conditions Wave 1 (2001/2002) and Wave 2 (2004/2005). The sample was restricted to participants who did not report hypertension at baseline (n = 26 844; 51% 40 years and older, 51% female, 71% white). χ2‐tests were used to examine the distributions of confounders stratified by the incidence of hypertension. Thereafter, multiple logistic regression analyses were conducted to quantify the relationships between lifetime cannabis use, 12‐month cannabis use and 12‐month cannabis use frequency and incidence of hypertension while adjusting for confounders.ResultsCannabis use was associated with a decreased incidence of hypertension in the unadjusted analyses. However, the relationships were confounded by age. After adjustment for all confounders, neither lifetime cannabis use (odds ratio, 95% confidence interval 0.89, 0.77 to 1.02), 12‐month cannabis use (0.78, 0.56 to 1.09) nor 12‐month cannabis use frequency [at least monthly use (0.85, 0.57 to 1.28) and less than monthly use (0.67, 0.40 to 1.11)] were associated above chance with the incidence of hypertension.Discussion and ConclusionsLifetime cannabis use, 12‐month cannabis use and 12‐month cannabis use frequency were not associated with the incidence of hypertension.
Hauling up a hefty meal: Long‐Jawed spider (Araneae, Tetragnathidae) uses silk lines to transport large prey vertically through the air in the absence of a web
Authors: Gould John 
Published: March 2021

AbstractThe most well‐known use of silk among spiders is the formation of webs to capture flying prey. However, spiders have evolved many different foraging strategies involving silk, including the capture and subsequent manipulate of prey prior to consumption. Herein, I report on the use of silk lines by a long‐jawed spider from the Tetragnatha genus to move a large prey item vertically through the air. Field observations revealed a long‐jawed spider attaching multiple silk lines across the body of an adult dragonfly that had recently emerged from its final moult and attaching these threads to overlying vegetation to incrementally haul the prey item from the surface of a waterbody. My observations suggest that some Tetragnatha spiders are able to move large prey items that are much heavier then themselves using a series of silk lines that allow lift to be accomplished in a gradual and controlled manner. This is an interesting finding, given that other individuals from the same population were using web structures to passively catch much smaller, flying prey. This indicates that some Tetragnatha spiders not only participate in the mobile pursuit of prey but that they possess multiple foraging strategies that may allow them to exploit a larger number of prey types.
Reservoir fertilisation and fishery response in a highly managed reservoir with uncertain flows: Ecosystem‐based management using decision analysis
Authors: Woodruff Patricia , van Poorten Brett T. , Christensen Villy , Walters Carl J. 
Published: March 2021

AbstractInland fisheries managers must account for multiple competing uses for aquatic resources; using methods such as ecosystem‐based management allows for different priorities for aquatic ecosystems to be accounted for. Declining abundance of kokanee salmon Oncorhynchus nerka (Walbaum) in Arrow Lakes Reservoir in the 1990s led to the use of large‐scale nutrient addition to improve productivity of kokanee and large piscivores. However, it is unclear what effect these measures had on the system given high discharge and highly variable annual flow regime throughout the watershed. An Ecopath with Ecosim model of the ecosystem was fitted to the available data and used to predict ecosystem structure and reservoir objectives under different nutrient addition strategies and varying annual flow regimes. Results from the model indicate that nutrient addition is an important driver in the system, with lower flows resulting in higher biomass for higher trophic levels. Decision analysis demonstrated the importance of maintaining nutrient additions to achieve management objectives despite losses in some high‐flow years.
Effect of sonication on microwave inactivation of Escherichia coli in an orange juice beverage
Authors: Kernou Ourdia‐Nouara , Belbahi Amine , Amir Akila , Bedjaoui Kenza , Kerdouche Kamelia , Dairi Sofiane , Aoun Omar , Madani Khodir 
Published: March 2021

AbstractThe survival of Escherichia coli ATCC 25922 in orange juice treated with microwave and/or ultrasound was evaluated; Weibull model was fitted to survival curves to describe inactivation kinetics; and the effect of combined microwave‐ultrasound treatments was assessed. Ultrasonic treatment (42 kHz) has no significant effect on the survival rate reduction (1.3 log for 60 min of sonication) of E. coli. However, sonication can increase the efficiency of microwave inactivation. The use of ultrasound as pre‐treatment was more effective than when used in post‐treatment, that is, a reduction of 8.0 log was achieved by a combination of ultrasound (20 min) followed by a microwave (900 W/30 s), while 4.0 log was obtained when these both processes were reversed. The scale parameter α estimated from microwave‐inactivation kinetics combined with an ultrasound pre‐treatment was found to be lower than those obtained with a post‐treatment, which, in turn, are lower than those estimated for microwave or ultrasound alone. These results demonstrate the effectiveness of ultrasound as a pre‐treatment to improve the inactivation of E. coli by microwave processing in orange juice beverages.Practical applicationsHigh‐temperature short‐time (HTST) type processes are preferred by the food industry to reduce the adverse thermal degradation in food quality while ensuring food safety for liquid food. The microwave heating is fast and can significantly reduce the come‐up time to the desired process temperature. The hydrodynamic action of ultrasound on microorganisms is a great potential to improve microbial decontamination efficiency by microwave processing. This study contributes to the design and control of an effective combination treatment of ultrasound and microwave to improve pasteurization processes for orange juice beverages.
GENDER DIVERSITY IN RESEARCH TEAMS AND CITATION IMPACT IN ECONOMICS AND MANAGEMENT
Authors: Maddi Abdelghani , Gingras Yves 
Published: March 2021

AbstractThe aim of this paper is twofold: (1) contribute to a better understanding of the place of women in Economics and Management disciplines by characterizing the difference in levels of scientific collaboration between men and women at the specialties’ level; and (2) investigate the relationship between gender diversity and citation impact in Economics and Management. Our data, extracted from the Web of Science database, cover global production as indexed in 302 journals in Economics and 370 journals in Management, with, respectively, 153,667 and 163,567 articles published between 2008 and 2018. Results show that collaborative practices between men and women are quite different in Economics and Management. We also find that there is a modest positive and significant effect of gender diversity on the citation impact of publications. Mixed‐gender publications (coauthored by men and women) receive more citations than nonmixed papers (written by same‐gender author teams) or single‐author publications. The regression analysis also indicates that there is, for Economics, a small negative effect on citations received if the corresponding author is a woman. Finally, the country (affiliation) of the corresponding author affects the citations received in the two disciplines.
National level adjustments to the challenges of the SARS‐CoV2 pandemic on blood banking operations
Authors: Matusovits Andrea , Nagy Sándor , Baróti‐Tóth Klára , Nacsa János , Lázár Mária , Marton Imelda , Andrikovics Hajnalka , Vokó Zoltan , Tordai Attila 
Published: March 2021

AbstractBackgroundSARS‐CoV2 causing coronavirus disease (COVID‐19) is responsible for an unprecedented worldwide pandemic severely affecting all activities of societies including blood banking. We aimed to systematically collect key indicators in a nationally centralized blood banking system and to perform comparisons between 2020 and 2019.MethodsCount data for January–December 2020 and 2019 were extracted from the integrated informatics system of Hungarian National Blood Transfusion Service and analyzed by simple graphics, tabulations, and statistics.ResultsWhole blood donation activity showed a highly significant decline due to a sharp decrease in field donations by an average fall of 24% (range:17%–28%) during March–May 2020 compared to identical period of 2019. A second, more moderate decline accompanied the second wave in late fall. The simultaneous increase in institutional donations did not counterbalance this decline. Donor exclusion rates fell significantly by an average of 1,1% (range:0.9%–1.6%) in the three spring lockdown‐affected months. First‐time and repeat donors showed decreased turn‐out in larger proportions compared to highly repeat donors. Interestingly, among repeat and highly repeat donors, females showed less‐pronounced declines compared to males while this was not observed among first‐time donors. In June–September, a remarkable swing‐back was observed among highly repeat female donors. Product utilization fell most notably for RBC (mean:26.2%) but also for PLT (mean:19.8%) and FFP (mean:24.3%) and showed a full recovery in June–September followed by a second decline.ConclusionTrends and reaction patterns of blood banking reported by our study may be useful in future planning and adjustments of blood banking activities.
Evaluating the health effect of a Social Housing programme, Minha Casa Minha Vida, using the 100 million Brazilian Cohort: a natural experiment study protocol
Authors: Ferreira Andrêa J F , Pescarini Julia , Sanchez Mauro , Flores-Ortiz Renzo Joel , Teixeira Camila Silveira , Fiaccone Rosemeire , Ichihara Maria Yury , Oliveira Rodrigo , Aquino Estela M L , Smeeth Liam , Craig Peter , Ali Sanni , Leyland Alastair H , Barreto Mauricio L , Ribeiro Rita de Cássia , Katikireddi Srinivasa Vittal 
Published: March 2021

IntroductionSocial housing programmes have been shown to influence health, but their effects on cardiovascular mortality and incidence of infectious diseases, such as leprosy and tuberculosis, are unknown. We will use individual administrative data to evaluate the effect of the Brazilian housing programme Minha Casa Minha Vida (MCMV) on cardiovascular disease (CVD) mortality and incidence of leprosy and tuberculosis.Methods and analysisWe will link the baseline of the 100 Million Brazilian Cohort (2001–2015), which includes information on socioeconomic and demographic variables, to the MCMV (2009–2015), CVD mortality (2007–2015), leprosy (2007–2015) and tuberculosis (2007–2015) registries. We will define our exposed population as individuals who signed the contract to receive a house from MCMV, and our non-exposed group will be comparable individuals within the cohort who have not signed a contract for a house at that time. We will estimate the effect of MCMV on health outcomes using different propensity score approaches to control for observed confounders. Follow-up time of individuals will begin at the date of exposure ascertainment and will end at the time a specific outcome occurs, date of death or end of follow-up (31 December 2015). In addition, we will conduct stratified analyses by the follow-up time, age group, race/ethnicity, gender and socioeconomic position.Ethics and disseminationThe study was approved by the ethic committees from Instituto Gonçalo Muniz-Oswaldo Cruz Foundation and University of Glasgow Medical, Veterinary and Life Sciences College. Data analysis will be carried out using an anonymised dataset, accessed by researchers in a secure computational environment according to the Centre for Integration of Data and Health Knowledge procedures. Study findings will be published in high quality peer-reviewed research journals and will also be disseminated to policy makers through stakeholder events and policy briefs.