bluuebunny/crossref_metadata_2025_split huggingface dataset:
Association Between Dehydroepiandrosterone Sulphate Levels at 7 Years Old And Bone Mineral Density At 10 Years Old – a Prospective Cohort Study
Authors: Santos-Silva Rita , Fontoura Manuel , Severo Milton , Lucas Raquel , Santos Ana Cristina 
Published: November 2021

Abstract
We aimed to explore the effect of dehydroepiandrosterone sulphate (DHEAS) at age 7 on areal bone mineral density (aBMD) at age 10, and to distinguish the direct and indirect effects (explained by sexual maturity and by aBMD at age 7), for each sex, after adjustment for body mass index z-score (BMI). In a subsample of 274 children (139 girls, 135 boys) from the Generation XXI cohort, aBMD was assessed with dual-energy x-ray absorptiometry (DXA) scan at age 7 and age 10. The increase in aBMD at age 10 for each 10 µg/dL increase in DHEAS levels at age 7 was estimated using path analysis. Both the direct and the indirect effects were calculated.In girls, higher DHEAS levels at age 7 were associated with higher aBMD at age 10. No direct effect was observed. The indirect effect via higher aBMD at age 7 explained 61% of the total effect, and the indirect effect via higher Tanner stage explained 21%. After adjustment for BMI, the total effect remained statistically significant, explained in 33% by the indirect effect of DHEAS on Tanner stage and Tanner stage on aBMD. In boys, no effect of DHEAS on aBMD was observed.Conclusion: An indirect effect of DHEAS at age 7 on aBMD at age 10 was found in girls, but not in boys, as higher DHEAS levels were associated with more advanced sexual maturity at age 10, and more advanced sexual maturity to higher aBMD. No direct effect of DHEAS on aBMD was observed.
Characterization of Triatomine Bloodmeal Sources Using Direct Sanger Sequencing and Amplicon Deep Sequencing Methods
Authors: Balasubramanian Sujata , Curtis-Robles Rachel , Chirra Bhagath , Auckland Lisa D. , Mai Alan , Bocanegra-Garcia Virgilio , Clark Patti , Clark Wilhelmina , Cottingham Mark , Fleurie Geraldine , Johnson Charles D. , Metz Richard P. , Wang Shichen , Hathaway Nicholas J. , Bailey Jeffrey A. , Hamer Gabriel L. , Hamer Sarah A. 
Published: November 2021

Abstract
Knowledge of host associations of blood-feeding vectors may afford insights into managing disease systems and protecting public health. However, the ability of methods to distinguish bloodmeal sources varies widely. We used two methods—Sanger sequencing and amplicon deep sequencing—to target a 228 bp region of the vertebrate CYTB gene and determine hosts fed upon by triatomines (n = 115) collected primarily in Texas, USA. Direct sanger sequencing of PCR amplicons was successful for 36 samples (31%). Sanger sequencing revealed 15 distinct host species, which included humans, domestic animals (Canis lupus familiaris, Ovis aries, Gallus gallus, Bos taurus, Felis catus, and Capra hircus), wildlife (Rattus rattus, Bufo nebulifer, Sciurus carolinensis, Sciurus niger, Odocoileus virginianus), and captive animals (Panthera tigris, Colobus spp., Chelonoidis carbonarius). Samples sequenced by the Sanger method were also subjected to Illumina MiSeq amplicon deep sequencing. The amplicon deep sequencing results (average of 302,080 usable reads per sample) replicated the host community revealed using Sanger sequencing, and detected additional hosts in five triatomines (13.9%), including two additional blood sources (Procyon lotor, Bassariscus astutus). Up to four bloodmeal sources were detected in a single triatomine (Bufo nebulifer, Homo sapiens, Canis lupus familiaris, and Sciurus carolinensis). Enhanced understanding of vector-host-parasite networks may allow for integrated vector management programs focusing on highly-utilized and highly-infected host species.
The Effect of Inorganic Salt on the Morphology and Nucleation of Polyaniline
Authors: jing Yiqi , Zhang Shujia , Pei Yuanjiao , Zeng Yufeng , Wang Ruijuan , Xu Zhuohui 
Published: November 2021

Abstract
Polyaniline nanofibers were fabricated through the addition of inorganic salt such as NaCl, MgSO4 and AlCl3 into the micelle-like composed of aniline and camphor sulfonic acid (CSA). The influence of types and concentration of inorganic salts, doped acids and temperature on polyaniline was studied by TEM, Uv-vis and FTIR spectroscopy. In addition, in situ Uv-vis and 1H NMR were applied to observe the process of aniline polymerization, and it was indicated the polymerization rate of aniline changed after the addition of inorganic salt NaCl into the initial solution.
Study On the Mechanism of LMP2A Maintaining Epstein-barr Virus Latency Infection Through Interaction With CXCR4
Authors: Qin Ni , Zhang Yan , Xu Lin , Liu Wen , Luo Bing 
Published: November 2021

Abstract
Epstein-Barr virus (EBV) belongs to the γ-herpesvirus subfamily and is the first human tumor virus to be discovered. The global adult infection rate exceeds 90%. EBV can participate in the regulation of multiple genes and multiple signal pathways through its latent genes. Many studies have reported that CXCR4 is involved in the development of gastric cancer, but there are few studies on the specific mechanism of its role in EBV-associated gastric cancer (EBVaGC). In this study, we explored the mechanism by which EBV-encoded products maintain EBV latent infection through interaction with CXCR4, and the role of CXCR4 in EBV positive cells. The results show that there is a positive feedback between the EBV-encoded products and CXCR4, and LMP2A can activate CXCR4 through the NF-κB pathway. In addition, CXCR4 can be fed back to LMP2A and EBNA1 through the ERK signaling pathway. At the same time, CXCR4 can promote the proliferation and migration of EBV-positive cells, reduce the expression of the immediate early protein BZLF1, and play an important role in maintaining the incubation period of EBV infection. These findings are conducive to the further targeted therapy of EBVaGC.
Wavefront Sensing of Interference Fringe Based On Generative Adversarial Network
Authors: Whang Allen Jong-Woei , Chen Yi-Yung , Chen His-Chi , Lin Chengtse , Yang Tsai-Hsien , Jian Zhi-Jia , Chou Chun-Han 
Published: November 2021

Abstract
To increase the measurement accuracy of optical systems, which are implemented in various applications, an improvement of the optical measurement technique is required. This paper proposes an image-to-image wavefront sensing approach using a deep neural network that directly predicts the phase image from the corresponding interference fringe image instead of reconstruction by the Zernike coefficients. The model is based on a conditional generative adversarial network (CGAN). To train the model, we used the formula-based ideal interference fringe images as the inputs of the CGAN, to conditionally predict the corresponding phase images as the output. We numerically investigated the performance by calculating the similarity between the ideal phase image and model output. In addition, with reference to a previous study, it was determined whether the model can extract more features from the interferogram for the prediction of Zernike coefficients. Moreover, an optical simulation software was introduced to provide an increased number of actual interferograms, to verify the proposed method. Based on the results, the proposed system can obtain the phase image directly and reduce the error, thus improving the measurement accuracy of the interference fringe.
Measures of Uncertainty for a Four-Hybrid Information System and Their Applications
Authors: Qin Bin , Zeng Fanping , Yan Kesong 
Published: November 2021

Abstract
A four-hybrid information system (4HIS) is an information system (IS) where the dataset of object descriptions consists of categorical, boolean, real-valued and missing data or attributes. This paper studies measures of uncertainty for a 4HIS and its application in attribute reduction. The distance function for each type of attribute in a 4HIS is first provided. Then, this distance is used to produce the tolerance relation induced by a given subsystem in a 4HIS. Next, information structure of this subsystem is proposed in terms of a set vector and dependence between information structures is introduced. Moreover, granulation and entropy measures in a 4HIS are investigated on the basis of information structures. In order to verify the feasibility of the proposed measures, effectiveness analysis is performed from a statistical perspective. Finally, an application of the proposed measures for attribute reduction in a 4HIS is given.
The Effect of Phasic Versus Combined Neuromuscular Electrical Stimulation Using the StimaWELL 120MTRS System on Multifidus Muscle Morphology and Function in Patients with Chronic Low Back Pain: A Randomized Pilot Control Trial Protocol
Authors: Fortin Maryse , Wolfe Daniel , Dover Geoffrey , Boily Mathieu 
Published: November 2021

Abstract
BackgroundNeuromuscular electrical stimulation (NMES) is used to improve muscle strength clinically when rehabilitating various musculoskeletal disorders. However, the effects of NMES on muscle morphology and function in individuals with non-specific chronic low back pain (CLBP) have scarcely been investigated. Although research links deficits in the paraspinal musculature with subjective reports of pain and disability, it is unknown if treatment with NMES can help reverse these deficits. Therefore, the primary aim of this study is to compare the effects of two muscle therapy protocols with a medium-frequency electrotherapy device (the StimaWELL 120MTRS system) on multifidus muscle morphology in CLBP patients. The secondary aims are to determine the effects of these protocols on multifidus muscle function, as well as subjective reports of pain intensity, pain interference, disability, and catastrophizing. MethodsA total of 30 participants with non-specific CLBP, aged 18-60, will be recruited from local orthopedic clinics and databases. Participants will be randomized (1:1) to either the phasic or combined (phasic + tonic) muscle therapy protocols on the StimaWELL 120MTRS system. Participants will undergo 20 supervised electrotherapy treatments over a 10-week period. The primary outcomes will be multifidus cross-sectional area (CSA) and fat infiltration. Secondary outcomes will include multifidus contraction (measured via %thickness change from a rested to contracted state), multifidus stiffness (at rest, and during contraction), as well as pain intensity, interference, disability, and catastrophizing. Both primary and secondary outcomes will be obtained at baseline and at 11-weeks; secondary outcomes measured via questionnaires will also be obtained at 6-weeks, while low back pain intensity will be measured before and after each treatment. Paired t-tests will be used to assess within-group changes for all primary outcome measures. A two-way repeated-measures analysis of variance will be use to assess changes in secondary outcomes over time. DiscussionThe results of this trial will help clarify the role of medium-frequency NMES on lumbar multifidus morphology and function. Trial RegistrationNCT04891692 Trial SponsorConcordia University, Montreal, Quebec, Canada
The Influence of Age, Sex and Exercise on Autophagy, Mitophagy and Lysosome Biogenesis in Skeletal Muscle
Authors: Triolo Matthew , Oliveria Ashley N. , Kumari Rita , Hood David A. 
Published: November 2021

Abstract
BackgroundAging decreases skeletal muscle mass and quality. Maintenance of healthy muscle is regulated by a balance between protein and organellar synthesis and their degradation. The autophagy lysosome system is responsible for the selective degradation of protein aggregates and organelles, such as mitochondria (i.e., mitophagy). Little data exist on the independent and combined influence of age, biological sex and exercise on the autophagy system and lysosome biogenesis. The purpose of this study was to characterize sex differences in autophagy and lysosome biogenesis in young and aged muscle, and to determine if acute exercise influences these processes.MethodsYoung (4-6 months) and aged (22-24 months) male and female mice, were assigned to a sedentary, or an acute exercise group. Mitochondrial content, the autophagy-lysosome system and mitophagy were measured via protein analysis. A Tfeb-promoter-construct was utilized to examine Tfeb transcription, and nuclear-cytosolic fractions allowed us to examine Tfeb localization in sedentary and exercised muscle with age and sex.ResultsOur results indicate that female mice, both young and old, had more mitochondrial protein than age-matched males, and mitochondrial content was only reduced with age in the male cohort. Although young female mice had a greater abundance of autophagy, mitophagy and lysosome proteins than young males, we measured increases with age irrespective of sex. Interestingly, young sedentary female mice had indices of greater autophagosomal turnover than male counterparts. Exhaustive exercise was able to stimulate autophagic clearance in young male mice, but not in the other groups. Similarly, nuclear Tfeb protein was enhanced to a greater extent in young male than in young female mice following exercise, but no changes were observed in aged mice. Finally, Tfeb-promoter activity was upregulated following exercise in both young and aged muscle.ConclusionsThe present study demonstrates that biological sex influences mitochondrial homeostasis, the autophagy-lysosome system and mitophagy in skeletal muscle with age. Further, our data suggest that young male mice have a more profound ability to activate these processes with exercise than in the other groups. Ultimately, this may contribute to a greater remodeling of muscle in response to exercise training in males.
The Prognostic Value of Metabolic Genes in Gastric Adenocarcinoma
Authors: Huang Sanling , Liu Zefu , Xu Fangzhou , Chen Chongxiang , Zhang Hongyu 
Published: November 2021

Abstract
Background: A great number of metabolic genes have been discovered in gastric cancer (GC); however, their prognostic roles remain incompletely clear so far. Methods: The Cancer Genome Atlas-stomach adenocarcinoma, and GSE84437 dataset collected via the Gene Expression Omnibus (GEO) database were utilized for retrieving those clinicopathological data and RNA expression patterns. Besides, the signature was obtained through the lasso Cox regression model and univariate Cox regression analysis. A novel 13-gene metabolic signature (including GSTA2, POLD3, GLA, GGT5, DCK, CKMT2, ASAH1, OPLAH, ME1, ACYP1, NNMT, POLR1A, and RDH12) was constructed for predicting the prognosis for gastric adenocarcinoma. Results: The results suggested that, the survival in high risk group was remarkably dismal compared with that in low risk group. In addition, that as-constructed signature had been identified as a factor to independently predict the prognosis for gastric adenocarcinoma patients. Moreover, the signature-based nomogram showed certain benefits in predicting the overall survival (OS). Besides, results of gene set enrichment analysis (GSEA) suggested that some pathways were markedly enriched, which help to illustrate the possible mechanisms. Conclusions: The new 13-gene metabolic model is constructed in this study to predict the prognosis for GC. It probably reflects dysregulation in the metabolic microenvironment, in the meantime of providing metabolic treatment biomarkers, and predicting the treatment response to gastric adenocarcinoma.
A Novel Dynamic of Localized Solitary Waves for the Hirota-Maccari System
Authors: Xia Pei , Zhang Yi , Zhang Heyan , Zhuang Yindong 
Published: November 2021

Abstract
This paper investigates a particular family of semi-rational solutions in determinant form by using the KP hierarchy reduction method, which describe resonant collisions among lumps or resemble line rogue waves and dark solitons in the Hirota-Maccari system. Due to the resonant collisions, the line resemble rogue waves are generated and attenuated in the background of dark solitons with line profiles of finite length, it takes a short time for the lumps to appear from and disappear into the dark solitons background. These novel dynamic of localized solitary waves may be help to understand some physical phenomena of nonlinear localized waves propagation in many physical settings.
Differential Regulation of Degradation and Immune Pathways Underlies Adaptation of the Ectosymbiotic Nematode Laxus Oneistus to Oxic-Anoxic Interfaces
Authors: Paredes Gabriela F. , Viehboeck Tobias , Markert Stephanie , Mausz Michaela A. , Sato Yui , Liebeke Manuel , König Lena , Bulgheresi Silvia 
Published: November 2021

Abstract
Eukaryotes may experience oxygen deprivation under both physiological and pathological conditions. Because oxygen shortage leads to a reduction in cellular energy production, all eukaryotes studied so far conserve energy by suppressing their metabolism. However, the molecular physiology of animals that naturally and repeatedly experience anoxia is underexplored. One such animal is the marine nematode Laxus oneistus. It thrives, invariably coated by its sulfur-oxidizing symbiont Candidatus Thiosymbion oneisti, in anoxic sulfidic or hypoxic sand. Here, transcriptomics and proteomics showed that, whether in anoxia or not, L. oneistus mostly expressed genes involved in ubiquitination, energy generation, oxidative stress response, immune response, development, and translation. Importantly, ubiquitination genes were also highly expressed when the nematode was subjected to anoxic sulfidic conditions, together with genes involved in autophagy, detoxification and ribosome biogenesis. We hypothesize that these degradation pathways were induced to recycle damaged cellular components (mitochondria) and misfolded proteins into nutrients. Remarkably, when L. oneistus was subjected to anoxic sulfidic conditions, lectin and mucin genes were also upregulated, potentially to promote the attachment of its thiotrophic symbiont. Furthermore, the nematode appeared to survive oxygen deprivation by using an alternative electron carrier (rhodoquinone) and acceptor (fumarate), to rewire the electron transfer chain. On the other hand, under hypoxia, genes involved in costly processes (e.g., amino acid biosynthesis, development, feeding, mating) were upregulated, together with the worm’s Toll-like innate immunity pathway and several immune effectors (e.g., Bacterial Permeability Increasing proteins, fungicides). In conclusion, we hypothesize that, in anoxic sulfidic sand, L. oneistus upregulates degradation processes, rewires oxidative phosphorylation and by reinforces its coat of bacterial sulfur-oxidizers. In upper sand layers, instead, it appears to produce broad-range antimicrobials and to exploit oxygen for biosynthesis and development.
Therapeutic effect of adipose stromal vascular fraction spheroids for partial bladder outlet obstruction induced underactive bladder
Authors: Liu Jingyu , Zhou Liuhua , Zhao Feng , Zhou Changcheng , Yang Tianli , Xu Zhongle , Wang Xinning , Xu Luwei , Xu Zheng , Ge Yuzheng , Wu Ran , Jia Ruipeng 
Published: November 2021

Abstract
Background: Underactive bladder (UAB) is a common clinical problem but related research is rarely explored. As there are currently no effective therapies, the administration of adipose stromal vascular fraction (ad-SVF) provides a new potential method to treat underactive bladder. Methods: Male Sprague-Dawley rats were induced by partial bladder outlet obstruction (PBOO) for four weeks and randomly divided into three groups: rats treated with PBS (Sham group); rats administrated with ad-SVF (ad-SVF group) and rats performed with ad-SVF spheroids (ad-SVFsp group). After four weeks, urodynamic studies were performed to evaluate bladder functions and all rats were sacrificed for further studies.Results: We observed that the bladder functions and symptoms of UAB were significantly improved in the ad-SVFsp group than that in the Sham group and ad-SVF group. Meanwhile, our data showed that ad-SVF spheroids could remarkably promote angiogenesis, suppress cell apoptosis and stimulate cell proliferation in bladder tissue than that in the other two groups. Moreover, ad-SVF spheroids increased the expression levels of bFGF, HGF and VEGF-A than ad-SVF. IVIS Spectrum small-animal in vivo imaging system revealed that ad-SVF spheroids could increase the retention rate of transplanted cells in bladder tissue. Conclusions: Ad-SVF spheroids improved functions and symptoms of bladder induced by PBOO, which contributes to promote angiogenesis, suppress cell apoptosis and stimulate cell proliferation. Ad-SVF spheroids provide a potential treatment for the future patients with UAB.
Impact of the serum and blood biomarkers on the severity and survival of the COVID-19 infected patients with dementia
Authors: Aydemir Duygu , Ulusu Nuriye Nuray 
Published: November 2021

Abstract
Aim

Elderly population is categorized as a risk group for COVID-19 infection and dementia is the major cause of disability in elderly individuals and affects 70% of the elderly population. In this study, we evaluated blood and serum biomarkers of the patients with dementia infected by COVID-19 to evaluate possible indicators of the severity of COVID-19 infection.
Methods

Laboratory biomarkers of 11 dementia patients between the ages 85-96 infected by COVID-19 have been used for this study. Serum biochemistry and blood data of survived six patients were compared with the five patients who died because of COVID-19 to evaluate biomarkers correlated with COVID-19 severity and disease mortality.
Results

Fibrinogen, d-dimer, C-reactive protein (CRP), P, and Mg levels increased in the deceased dementia patients compared to the survived ones. Glucose, blood urea nitrogen (BUN), alanine transaminase (ALT), aspartate aminotransferase (AST), troponin, lactate, and procalcitonin levels significantly decreased in the deceased patients compared to the survived ones infected by COVID-19. %NEU, %LYM, MONO, %MONO, EOS, %EOS, %BASO, MPV, PT, INR, hematocrit (HCT), hemoglobin (Hb), total Hb, red blood cells (RBC), PDW, and ferritin levels decreased in the deceased patients compared to the healthy ones, where red cell distribution width (RDW), prothrombin time (PT), WBC and NEU levels significantly increased in the deceased patients infected by COVID-19.
Conclusion

Changes in the serum biochemistry and blood markers are correlated with COVID-19 infection severity and mortality that can be used to the prediction of disease progression in dementia patients.