bluuebunny/crossref_metadata_2025_split huggingface dataset:
Activation of meiotic recombination by nuclear import of the DNA break hotspot-determining complex in fission yeast
Authors: Wintrebert Mélody , Nguyen Mai-Chi , Smith Gerald R. 
Published: February 2021

ABSTRACT
Meiotic recombination forms crossovers important for proper chromosome segregation and offspring viability. This complex process involves many proteins acting at each of the multiple steps of recombination. Recombination initiates by formation of DNA double-strand breaks (DSBs), which in the several species examined occur with high frequency at special sites (DSB hotspots). In Schizosaccharomyces pombe, DSB hotspots are bound with high specificity and strongly activated by linear element (LinE) proteins Rec25, Rec27 and Mug20, which form colocalized nuclear foci with Rec10, essential for all DSB formation and recombination. Here, we test the hypothesis that the nuclear localization signal (NLS) of Rec10 is crucial for coordinated nuclear entry after forming a complex with other LinE proteins. In NLS mutants, all LinE proteins were abundant in the cytoplasm, not the nucleus; DSB formation and recombination were much reduced but not eliminated. Nuclear entry of limited amounts of Rec10, apparently small enough for passive nuclear entry, can account for residual recombination. LinE proteins are related to synaptonemal complex proteins of other species, suggesting that they also share an NLS, not yet identified, and undergo protein complex formation before nuclear entry.
This article has an associated First Person interview with Mélody Wintrebert, joint first author of the paper.
Adenine nucleotide translocase regulates airway epithelial metabolism, surface hydration and ciliary function
Authors: Kliment Corrine R. , Nguyen Jennifer M. K. , Kaltreider Mary Jane , Lu YaWen , Claypool Steven M. , Radder Josiah E. , Sciurba Frank C. , Zhang Yingze , Gregory Alyssa D. , Iglesias Pablo A. , Sidhaye Venkataramana K. , Robinson Douglas N. 
Published: February 2021

ABSTRACT
Airway hydration and ciliary function are critical to airway homeostasis and dysregulated in chronic obstructive pulmonary disease (COPD), which is impacted by cigarette smoking and has no therapeutic options. We utilized a high-copy cDNA library genetic selection approach in the amoeba Dictyostelium discoideum to identify genetic protectors to cigarette smoke. Members of the mitochondrial ADP/ATP transporter family adenine nucleotide translocase (ANT) are protective against cigarette smoke in Dictyostelium and human bronchial epithelial cells. Gene expression of ANT2 is reduced in lung tissue from COPD patients and in a mouse smoking model, and overexpression of ANT1 and ANT2 resulted in enhanced oxidative respiration and ATP flux. In addition to the presence of ANT proteins in the mitochondria, they reside at the plasma membrane in airway epithelial cells and regulate airway homeostasis. ANT2 overexpression stimulates airway surface hydration by ATP and maintains ciliary beating after exposure to cigarette smoke, both of which are key functions of the airway. Our study highlights a potential for upregulation of ANT proteins and/or of their agonists in the protection from dysfunctional mitochondrial metabolism, airway hydration and ciliary motility in COPD.
This article has an associated First Person interview with the first author of the paper.
Different protein metabolic strategies for growth during food-induced physiological plasticity in echinoid larvae
Authors: Ellison Aimee , Pouv Amara , Pace Douglas A. 
Published: February 2021

ABSTRACTFood-induced morphological plasticity, a type of developmental plasticity, is a well-documented phenomenon in larvae of the echinoid echinoderm, Dendraster excentricus. A recent study in our lab has shown that this morphological plasticity is associated with significant physiological plasticity for growth. The goal of the current study was to measure several aspects of protein metabolism in larvae growing at different rates to understand the mechanistic basis for this physiological growth plasticity. Larvae of D. excentricus were fed rations of 1000 algal cells ml−1 (low-fed larvae) or 10,000 algal cells ml−1 (high-fed larvae). Relative protein growth rate was 6.0 and 12.2% day−1 for low- and high-fed larvae, respectively. The energetic cost of protein synthesis was similar for the two treatments at 4.91 J mg−1 protein synthesized. Larvae in both treatments used about 50% of their metabolic energy production to fuel protein synthesis. Mass-specific rates of protein synthesis were also similar. Large differences in mass-specific rates of protein degradation were observed. Low-fed larvae had relatively low rates of degradation early in development that increased with larval age, surpassing those of high-fed larvae at 20 days post-fertilization. Changes in protein depositional efficiency during development were similar to those of larval growth efficiency, indicating that differences in protein metabolism are largely responsible for whole-organism growth plasticity. Low-fed larvae also had alanine transport rates that were 2 times higher than those of high-fed larvae. In total, these results provide an explanation for the differences in growth efficiency between low- and high-fed larvae and allow for a more integrated understanding of developmental plasticity in echinoid larvae.
Sex-specific energy management strategies in response to training for increased foraging effort prior to reproduction in captive zebra finches
Authors: Yap Kang Nian , Powers Donald R. , Vermette Melissa L. , Tsai Olivia Hsin-I. , Williams Tony D. 
Published: February 2021

ABSTRACT
Free-living animals often engage in behaviour that involves high rates of workload and results in high daily energy expenditure (DEE), such as reproduction. However, the evidence for elevated DEE accompanying reproduction remains equivocal. In fact, many studies have found no difference in DEE between reproducing and non-reproducing females. One of the hypotheses explaining the lack of difference is the concept of an ‘energetic ceiling’. However, it is unclear whether the lack of increase in energy expenditure is due to the existence of an energetic ceiling and/or compensation by males during parental care. To investigate whether an energetic ceiling exists, we experimentally manipulated foraging effort in captive zebra finches, Taeniopygia guttata, creating two groups with high and low foraging efforts followed by both groups breeding in the low foraging effort common garden condition. DEE was measured in both sexes throughout the experiment. We show sex-specific energy management strategies in response to training for increased foraging effort prior to reproduction. Specifically, males and females responded differently to the high foraging effort treatment and subsequently to chick rearing in terms of energy expenditure. Our results also suggest that there is an energetic ceiling in females and that energetic costs incurred prior to reproduction can be carried over into subsequent stages of reproduction in a sex-specific manner.
Angling gear avoidance learning in juvenile red sea bream: evidence from individual-based experiments
Authors: Takahashi Kohji , Masuda Reiji 
Published: February 2021

ABSTRACT
Angling gear avoidance learning is a possible factor that contributes to the vulnerability of caught-and-released fish to angling. Whereas past studies suggested angling gear avoidance learning, they were based on large-scale experiments on groups of fish and unable to verify learning accurately. Details of avoidance learning are also unclear. The present study investigated angling gear avoidance learning through a series of individual-based experiments using red sea bream (Pagrus major) juveniles. Fish avoided angling gear after only one or two catches while showing feeding motivation for pellets, representing avoidance learning for angling gear. Most of the experienced fish avoided krill attached to a fishing line, but not krill alone or pellets presented near the angling gear. Experienced fish were less vulnerable to angling than control fish. Approximately half of the experienced fish kept the memory of angling gear 2 months after learning. The learning effect through the catch-and-release procedure would reduce catchability and the value of fishery-dependent stock assessments.
Neo-institutional approaches and primary research: The case study of EQUAL and LEADER local partnerships in Crete
Authors: Γρηγοριάδου (Despoina Grigoriadou) Δέσποινα 
Published: February 2021

This book acknowledges and develops the role of neo-institutional theories in understanding and interpreting political phenomena. In particular, this neo-institutional study analyses the dynamic interaction between formal/informal rules and agents’ behaviour inside a political institution, examining these relationships through primary research on local partnerships in Greece.
The theoretical assumptions of this analysis derive mainly from normative neo-institutionalism but also include insights from rational choice and the historical institutionalism. Consideration is also given to sociological theories of the structure/agent duality along with concepts and empirical observations from the literature on urban governance, urban regimes, Europeanisation and political leadership.
This interdisciplinary analysis stems epistemologically from the critical realism stance which acknowledges a reflexive approach to reality. This was also integrated into the research methodology with the implementation of an embedded case study strategy. The case study consists of two local partnerships in the region of Crete, which were established under the EU Community Initiatives Programmes EQUAL II and LEADER+ and coordinated by the Local Development Agency of Heraklion.
The research data reveal that the formal rules of the partnerships are not indicative of what actually happens. It is the configuration of formal and informal rules that offers a deep understanding of partnership. The research also shows the importance of agents' intentionality in the process of institutional change as well as the specific spatial and temporal context in which this institutional change takes place each time.
Elucidating the genetic architecture underlying IGF1 levels and its impact on genomic instability and cancer risk
Authors: Stankovic Stasa , R. Day Felix , Zhao Yajie , Langenberg Claudia , J. Wareham Nicholas ,   23andMe Research Team, R. B. Perry John , K. Ong Ken 
Published: February 2021

Background: Insulin-like growth factor-1 (IGF1) has been implicated in mitogenic and anti-apoptotic mechanisms that promote susceptibility to cancer development and growth. Previous epidemiological studies have described phenotypic associations between higher circulating levels of IGF1 in adults with higher risks for breast, prostate, ovarian, colorectal, melanoma and lung cancers. However, such evidence is prone to confounding and reverse causality. Furthermore, it is unclear whether IGF1 promotes only the survival and proliferation of cancerous cells, or also the malignant transformation of healthy cells. Methods: We perform a genome-wide association study in 428,525 white European ancestry individuals in the UK Biobank study (UKBB) and identify 831 independent genetic determinants of circulating IGF1 levels, double the number previously reported. Results: Collectively these signals explain ~7.5% of the variance in circulating IGF1 levels in EPIC-Norfolk, with individuals in the highest 10% of genetic risk exhibiting ~1 SD higher levels than those in the lowest 10%. Using a Mendelian randomization approach, we demonstrate that genetically higher circulating IGF1 levels are associated with greater likelihood of mosaic loss of chromosome Y in leukocytes in men in UKBB (OR per +1 SD = 1.038 (95% CI: 1.010-1.067), P=0.008) and 23andMe, Inc. (P=6.8×10-05), a biomarker of genomic instability involved in early tumorigenesis. Genetically higher IGF1 is also associated with higher risks for colorectal (OR = 1.126 (1.048-1.210), P=1.3×10-03) and breast cancer (OR= 1.075 (1.048-1.103), P=3.9×10-08), with similar effects on estrogen positive (ER+) (OR = 1.069 (1.037-1.102), P=2.3×10-05) and estrogen negative (ER-) (OR = 1.074 (1.025-1.125), P=3.9×10-08) subtypes. Conclusions: These findings give an insight into the genetic regulation of circulating IGF1 levels and support a causal role for IGF1 in early tumorigenesis and risks for breast and colorectal cancers.
Gender and Sibling Dynamics in the Intergenerational Transmission of Entrepreneurship
Authors: Mishkin Elizabeth 
Published: February 2021

This project uses gender and sibling dynamics to explore the intergenerational transmission of entrepreneurship. I find that the transmission of self-employment from fathers to daughters is significantly reduced when there are sons in the family. I interpret this as evidence that the intergenerational transmission of entrepreneurship is driven, at least in part, by costly investments by parents, which can be crowded out by or redirected toward brothers. I investigate specific types of parental investments—transfers of money, businesses, and human capital—that potentially underlie this transmission and conclude that sons reduce human-capital acquisition by daughters. If all daughters of self-employed men experienced the “sisters-only” level of transmission, the overall gender gap in self-employment would be reduced by roughly 15%. This paper was accepted by Toby Stuart, entrepreneurship and innovation.
Influence of team composition on turnover and efficiency of total hip and knee arthroplasty
Authors: Cahan Eli M. , Cousins Henry C. , Steere Joshua T. , Segovia Nicole A. , Miller Matthew D. , Amanatullah Derek F. 
Published: February 2021

Aims Surgical costs are a major component of healthcare expenditures in the USA. Intraoperative communication is a key factor contributing to patient outcomes. However, the effectiveness of communication is only partially determined by the surgeon, and understanding how non-surgeon personnel affect intraoperative communication is critical for the development of safe and cost-effective staffing guidelines. Operative efficiency is also dependent on high-functioning teams and can offer a proxy for effective communication in highly standardized procedures like primary total hip and knee arthroplasty. We aimed to evaluate how the composition and dynamics of surgical teams impact operative efficiency during arthroplasty. Methods We performed a retrospective review of staff characteristics and operating times for 112 surgeries (70 primary total hip arthroplasties (THAs) and 42 primary total knee arthroplasties (TKAs)) conducted by a single surgeon over a one-year period. Each surgery was evaluated in terms of operative duration, presence of surgeon-preferred staff, and turnover of trainees, nurses, and other non-surgical personnel, controlling cases for body mass index, presence of osteoarthritis, and American Society of Anesthesiologists (ASA) score. Results Turnover among specific types of operating room staff, including the anaesthesiologist (p = 0.011), circulating nurse (p = 0.027), and scrub nurse (p = 0.006), was significantly associated with increased operative duration. Furthermore, the presence of medical students and nursing students were associated with improved intraoperative efficiency in TKA (p = 0.048) and THA (p = 0.015), respectively. The presence of surgical fellows (p > 0.05), vendor representatives (p > 0.05), and physician assistants (p > 0.05) had no effect on intraoperative efficiency. Finally, the presence of the surgeon’s 'preferred' staff did not significantly shorten operative duration, except in the case of residents (p = 0.043). Conclusion Our findings suggest that active management of surgical team turnover and composition may provide a means of improving intraoperative efficiency during THA and TKA. Cite this article: Bone Joint J 2021;103-B(2):347–352.
Capsular resection versus capsular repair in direct anterior approach for total hip arthroplasty: a randomized controlled trial
Authors: Vandeputte Frans-Jozef , Vanbiervliet Jens , Sarac Cigdem , Driesen Ronald , Corten Kristoff 
Published: February 2021

Aims Optimal exposure through the direct anterior approach (DAA) for total hip arthroplasty (THA) conducted on a regular operating theatre table is achieved with a standardized capsular releasing sequence in which the anterior capsule can be preserved or resected. We hypothesized that clinical outcomes and implant positioning would not be different in case a capsular sparing (CS) technique would be compared to capsular resection (CR). Methods In this prospective trial, 219 hips in 190 patients were randomized to either the CS (n = 104) or CR (n = 115) cohort. In the CS cohort, a medial based anterior flap was created and sutured back in place at the end of the procedure. The anterior capsule was resected in the CR cohort. Primary outcome was defined as the difference in patient-reported outcome measures (PROMs) after one year. PROMs (Harris Hip Score (HHS), Hip disability and Osteoarthritis Outcome Score (HOOS), and Short Form 36 Item Health Survey (SF-36)) were collected preoperatively and one year postoperatively. Radiological parameters were analyzed to assess implant positioning and implant ingrowth. Adverse events were monitored. Results At one year, there was no difference in HSS (p = 0.728), HOOS (Activity Daily Life, p = 0.347; Pain, p = 0.982; Quality of Life, p = 0.653; Sport, p = 0.994; Symptom, p = 0.459), or SF-36 (p = 0.338). Acetabular component inclination (p = 0.276) and anteversion (p = 0.392) as well as femoral component alignment (p = 0.351) were similar in both groups. There were no dislocations, readmissions, or reoperations in either group. The incidence of psoas tendinitis was six cases in the CS cohort (6%) and six cases in the CR cohort (5%) (p = 0.631). Conclusion No clinical differences were found between resection or preservation of the anterior capsule when performing a primary THA through the DAA on a regular theatre table. In case of limited visibility during the learning curve, it might be advisable to resect a part of the anterior capsule. Cite this article: Bone Joint J 2021;103-B(2):321–328.
Vancouver B periprosthetic fractures involving the Exeter cemented stem
Authors: Powell-Bowns Matilda F. R. , Oag Erlend , Ng Nathan , Pandit Hemant , Moran Matthew , Patton James T. , Clement Nick D. , Scott Chloe E. H. 
Published: February 2021

Aims The aim of this study was to determine whether fixation, as opposed to revision arthroplasty, can be safely used to treat reducible Vancouver B type fractures in association with a cemented collarless polished tapered femoral stem (the Exeter). Methods This retrospective cohort study assessed 152 operatively managed consecutive unilateral Vancouver B fractures involving Exeter stems; 130 were managed with open reduction and internal fixation (ORIF) and 22 with revision arthroplasty. Mean follow-up was 6.5 years (SD 2.6; 3.2 to 12.1). The primary outcome measure was revision of at least one component. Kaplan–Meier survival analysis was performed. Regression analysis was used to identify risk factors for revision following ORIF. Secondary outcomes included any reoperation, complications, blood transfusion, length of hospital stay, and mortality. Results Fractures (B1 n = 74 (49%); B2 n = 50 (33%); and B3 n = 28 (18%)) occurred at median of 4.2 years (interquartile range (IQR) 1.2 to 9.2) after primary total hip arthroplasty (THA) (n = 138) or hemiarthroplasty (n = 14). Rates of revision and reoperation were significantly higher following revision arthroplasty compared to ORIF for B2 (p = 0.001) and B3 fractures (p = 0.050). Five-year survival was significantly better following ORIF: 92% (95% confidence interval (CI) 86.4% to 97.4%) versus 63% (95% CI 41.7% to 83.3%), p < 0.001. ORIF was associated with reduced blood transfusion requirement and reoperations, but there were no differences in medical complications, hospital stay, or mortality between surgical groups. No independent predictors of revision following ORIF were identified: where the bone-cement interface was intact, fixation of B2 or B3 fractures was not associated with an increased risk of revision. Conclusion When the bone-cement interface was intact and the fracture was anatomically reducible, all Vancouver B fractures around Exeter stems could be managed with fixation as opposed to revision arthroplasty. Fixation was associated with reduced need for blood transfusion and lower risk of revision surgery compared with revision arthroplasty. Cite this article: Bone Joint J 2021;103-B(2):309–320.
Reassessing glenoid inclination in reverse total shoulder arthroplasty with glenosphere lateralization
Authors: Mahendraraj Kuhan A. , Shields Maggie V. , Grubhofer Florian , Golenbock Samuel W. , Jawa Andrew 
Published: February 2021

Aims Existing literature indicates that inferiorly inclined glenoid baseplates following reverse total shoulder arthroplasty (RSA) produce better outcomes compared to superiorly inclined baseplates. We aim to compare clinical outcomes for RSAs with superiorly and neutrally/inferiorly inclined lateralized glenospheres. Methods We retrospectively reviewed 154 consecutive patients undergoing RSA between July 2015 and July 2017 by one single-fellowship trained surgeon (AJ). Two raters (KAM and MVS) independently measured glenoid inclination in preoperative and minimum two year follow-up radiographs (anteroposterior/Grashey) using the RSA angle. Inclination was then compared to patient-reported outcomes, range of motion (ROM), and independently assessed degree of scapular notching and staging of heterotopic ossification at two year follow-up. Results Median postoperative inclination for each group was found to be -3.6° (interquartile range (IQR) -2.1 to -6.9) and 6.0° (3.2° to 10.1°) for the neutrally/inferiorly and superiorly inclined cohorts, respectively. Preoperative inclination was highly associated with postoperative inclination (p = 0.004). When comparing superiorly and neutrally/inferiorly inclined glenospheres, there were no differences in heterotopic ossification (p = 0.606), scapular notching (p = 0.367), American Shoulder and Elbow Surgeons score (p = 0.419), Single Assessment Numeric Evaluation (p = 0.417), Visual Analogue Scale (VAS) pain score (p = 0.290), forward elevation (p = 0.161), external rotation (p = 0.537), or internal rotation (p = 0.656). Conclusion Compared to neutral and inferior inclination, up to 6° ± 3° of superior glenoid baseplate inclination on a lateralized RSA design produces no differences in postoperative ROM or patient-reported outcomes, and produces similar levels of scapular notching and heterotopic ossification. Additionally, the degree of preoperative inclination represents an important factor in surgical decision-making as it is strongly associated with postoperative inclination. It is important to note that the findings of this study are only reflective of lateralized RSA prostheses. Cite this article: Bone Joint J 2021;103-B(2):360–365.