bluuebunny/crossref_metadata_2025_split huggingface dataset:
Incidence of cardiovascular events and mortality in Korean patients with chronic kidney disease
Authors: Ryu Hyunjin , Kim Jayoun , Kang Eunjeong , Hong Yeji , Chae Dong-Wan , Choi Kyu Hun , Han Seung Hyeok , Yoo Tae Hyun , Lee Kyubeck , Kim Yong-Soo , Chung Wookyung , Oh Yun Kyu , Kim Soo Wan , Kim Yeong Hoon , Sung Su Ah , Lee Joongyub , Park Sue K. , Ahn Curie , Oh Kook-Hwan ,   Representing KNOW-CKD Study Group
Published: January 2021

AbstractFew studies have investigated the incidence of cardiovascular disease (CVD) in the Asian chronic kidney disease (CKD) population. This study assessed the incidence of CVD, death, and a composite outcome of CVD and death in a prospective Korean predialysis CKD cohort. From a total of 2179 patients, incidence rates were analyzed, and competing risk analyses were conducted according to CKD stage. Additionally, incidence was compared to the general population. During a median 4.1 years of follow-up, the incidence of CVD, all-cause death, and the composite outcome was 17.2, 9.6, and 24.5 per 1000 person-years, respectively. These values were higher in diabetic vs. non-diabetic subjects (P < 0.001). For all outcomes, incidence rates increased with increasing CKD stage (CVD, P = 0.001; death, P < 0.001; and composite, P < 0.001). Additionally, CKD stage G4 [hazard ratio (HR) 2.8, P = 0.008] and G5 (HR 5.0, P < 0.001) were significant risk factors for the composite outcome compared to stage G1 after adjustment. Compared to the general population, the total cohort population (stages G1–G5) showed significantly higher risk of CVD (HR 2.4, P < 0.001) and the composite outcome (HR 1.7, P < 0.001). The results clearly demonstrate that CKD is a risk factor for CVD in an Asian population.
Sphingolipids and physical function in the Atherosclerosis Risk in Communities (ARIC) study
Authors: Li Danni , Alam Aniqa B. , Yu Fang , Kucharska-Newton Anna , Windham B. Gwen , Alonso Alvaro 
Published: January 2021

AbstractLong-chain sphingomyelins (SMs) may play an important role in the stability of myelin sheath underlying physical function. The objective of this study was to examine the cross-sectional and longitudinal associations of long-chain SMs [SM (41:1), SM (41:2), SM (43:1)] and ceramides [Cer (41:1) and Cer (43:1)] with physical function in the Atherosclerosis Risk in Communities (ARIC) study. Plasma concentrations of SM (41:1), SM (41:2), SM (43:1), Cer (41:1) and Cer (43:1) were measured in 389 ARIC participants in 2011–13. Physical function was assessed by grip strength, Short Physical Performance Battery (SPPB), 4-m walking speed at both 2011–13 and 2016–17, and the modified Rosow-Breslau questionnaire in 2016–2017. Multivariable linear and logistic regression analyses were performed, controlling for demographic and clinical confounders. In cross-sectional analyses, plasma concentrations of SM 41:1 were positively associated with SPPB score (β-coefficients [95% confidence internal]: 0.33 [0.02, 0.63] per 1 standard deviation [SD] increase in log-transformed concentration, p value 0.04), 4-m walking speed (0.042 m/s [0.01, 0.07], p value 0.003), and negatively with self-reported disability (odds ratio = 0.73 [0.65, 0.82], p value < 0.0001). Plasma concentrations of the five metabolites examined were not significantly associated with longitudinal changes in physical function or incidence of poor mobility. In older adults, plasma concentrations of long-chain SM 41:1 were cross-sectionally positively associated with physical function.
Differential organ-specific inflammatory response to progranulin in high-fat diet-fed mice
Authors: Murakoshi Maki , Gohda Tomohito , Adachi Eri , Ichikawa Saki , Hagiwara Shinji , Suzuki Yusuke 
Published: January 2021

AbstractProgranulin (PGRN) has been reported to bind tumor necrosis factor (TNF) receptor and to inhibit TNFα signaling. We evaluated the effect of augmentation of TNFα signaling by PGRN deficiency on the progression of kidney injury. Eight-week-old PGRN knockout (KO) and wild-type (WT) mice were fed a standard diet or high-fat diet (HFD) for 12 weeks. Albuminuria, markers of tubular damage, and renal mRNA levels of inflammatory cytokines were higher in HFD-fed KO (KO-HFD) mice than in HFD-fed WT (WT-HFD) mice. Body weight, vacuolization in proximal tubules, and systemic and adipose tissue inflammatory markers were lower in the KO-HFD mice than in the WT-HFD mice. The renal megalin expression was lower in the KO mice than in the WT mice regardless of the diet type. The megalin expression was also reduced in mouse proximal tubule epithelial cells stimulated with TNFα and in those with PGRN knockdown by small interfering RNA in vitro. PGRN deficiency was associated with both exacerbated renal inflammation and decreased systemic inflammation, including that in the adipose tissue of mice with HFD-induced obesity. Improved tubular vacuolization in the KO-HFD mice might partially be explained by the decreased expression of megalin in proximal tubules.
Detectable chromosome X mosaicism in males is rarely tolerated in peripheral leukocytes
Authors: Zhou Weiyin , Lin Shu-Hong , Khan Sairah M. , Yeager Meredith , Chanock Stephen J. , Machiela Mitchell J. 
Published: January 2021

AbstractAge-related male Y and female X chromosome mosaicism is commonly observed in large population-based studies. To investigate the frequency of male X chromosome mosaicism, we scanned for deviations in chromosome X genotyping array intensity data in a population-based survey of 196,219 UK Biobank men. We detected 12 (0.006%) men with mosaic chromosome X gains ≥ 2 Mb and found no evidence for mosaic chromosome X loss, a level of detection substantially lower than for autosomes or other sex chromosomes. The rarity of chromosome X mosaicism in males relative to females reflects the importance of chromosome X gene dosage for leukocyte function.
An in vitro study on Staphylococcus schweitzeri virulence
Authors: Grossmann Almut , Froböse Neele J. , Mellmann Alexander , Alabi Abraham S. , Schaumburg Frieder , Niemann Silke 
Published: January 2021

AbstractStaphylococcus schweitzeri belongs to the Staphylococcus aureus-related complex and is mainly found in African wildlife; no infections in humans are reported yet. Hence, its medical importance is controversial. The aim of this work was to assess the virulence of S. schweitzeri in vitro. The capacity of African S. schweitzeri (n = 58) for invasion, intra- and extracellular cytotoxicity, phagolysosomal escape, coagulase activity, biofilm formation and host cell activation was compared with S. aureus representing the most common clonal complexes in Africa (CC15, CC121, CC152). Whole genome sequencing revealed that the S. schweitzeri isolates belonged to five geographical clusters. Isolates from humans were found in two different clades. S. schweitzeri and S. aureus showed a similar host cell invasion (0.9 vs. 1.2 CFU/Vero cell), host cell activation (i.e. expression of pro-inflammatory cytokines, 4.1 vs. 1.7 normalized fold change in gene expression of CCL5; 7.3 vs. 9.9 normalized fold change in gene expression of IL8, A549 cells) and intracellular cytotoxicity (31.5% vs. 25% cell death, A549 cells). The extracellular cytotoxicity (52.9% vs. 28.8% cell death, A549 cells) was higher for S. schweitzeri than for S. aureus. Nearly all tested S. schweitzeri (n = 18/20) were able to escape from phagolysosomes. In conclusion, some S. schweitzeri isolates display virulence phenotypes comparable to African S. aureus. S. schweitzeri might become an emerging zoonotic pathogen within the genus Staphylococcus.
The ectopic expression of Arabidopsis glucosyltransferase UGT74D1 affects leaf positioning through modulating indole-3-acetic acid homeostasis
Authors: Jin Shanghui , Hou Bingkai , Zhang Guizhi 
Published: January 2021

AbstractLeaf angle is an important agronomic trait affecting photosynthesis efficiency and crop yield. Although the mechanisms involved in the leaf angle control are intensively studied in monocots, factors contribute to the leaf angle in dicots are largely unknown. In this article, we explored the physiological roles of an Arabidopsis glucosyltransferase, UGT74D1, which have been proved to be indole-3-acetic acid (IAA) glucosyltransferase in vitro. We found that UGT74D1 possessed the enzymatic activity toward IAA glucosylation in vivo and its expression was induced by auxins. The ectopically expressed UGT74D1 obviously reduced the leaf angle with an altered IAA level, auxin distribution and cell size in leaf tissues. The expression of several key genes involved in the leaf shaping and leaf positioning, including PHYTOCHROME KINASE SUBSTRATE (PKS) genes and TEOSINTE BRANCHED1, CYCLOIDEA, and PCF (TCP) genes, were dramatically changed by ectopic expression of UGT74D1. In addition, clear transcription changes of YUCCA genes and other auxin related genes can be observed in overexpression lines. Taken together, our data indicate that glucosyltransferase UGT74D1 could affect leaf positioning through modulating auxin homeostasis and regulating transcription of PKS and TCP genes, suggesting a potential new role of UGT74D1 in regulation of leaf angle in dicot Arabidopsis.
Utility of Claudin-3 in extracellular vesicles from human bile as biomarkers of cholangiocarcinoma
Authors: Ikeda Chisaki , Haga Hiroaki , Makino Naohiko , Inuzuka Tatsutoshi , Kurimoto Ayako , Ueda Toshiki , Matsuda Akiko , Kakizaki Yasuharu , Ishizawa Tetsuya , Kobayashi Toshikazu , Sugahara Shinpei , Tsunoda Michihiko , Suda Kensei , Ueno Yoshiyuki 
Published: January 2021

AbstractExtracellular vesicles (EVs) are released from all cells. Bile directly contacts bile duct tumor; bile-derived EVs may contain high concentrations of cancer biomarkers. We performed a proteomic analysis of human bile-derived EVs and identified a novel biomarker of cholangiocarcinoma (CCA). EVs were isolated using ultracentrifugation, and chelating agents, ethylenediaminetetraacetic acid and ethylene glycol tetraacetic acid (EDEG) and phosphate buffered saline (PBS) were used as dissolution solutions. Bile was collected from 10 CCA and 10 choledocholithiasis (stones) cases. Proteomic analysis was performed; subsequently, ELISA was performed using the candidate biomarkers in a verification cohort. The vesicles isolated from bile had a typical size and morphology. The expression of exosome markers was observed. RNA was more abundant in the EDEG group. The proportion of microRNA was higher in the EDEG group. EDEG use resulted in the removal of more contaminants. Proteomic analysis identified 166 proteins as CCA-specific. ELISA for Claudin-3 revealed statistically significant difference. The diagnostic accuracy was AUC 0.945 and sensitivity and specificity were 87.5%. We report the first use of EDEG in the isolation of EVs from human bile and the proteomic analysis of human bile-derived EV-proteins in CCA. Claudin-3 in bile-derived EVs is a useful biomarker for CCA.
DUSP12 acts as a novel endogenous protective signal against hepatic ischemia–reperfusion damage by inhibiting ASK1 pathway
Authors: Boldorini Renzo , Clemente Nausicaa , Alchera Elisa , Carini Rita 
Published: January 2021

Abstract
Ischemia–reperfusion injury (IRI) consequent to major liver surgery is a still unmet clinical problem. The activation of endogenous systems of hepatoprotection can prevent the damaging effects of ischemia–reperfusion (IR) as shown by the phenomenon known as ‘ischemic preconditioning’. The identification of endogenous signal mediators of hepatoprotection is of main interest since they could be targeted in future therapeutic interventions. Qiu et al. recently reported in Clin. Sci. (Lond.) (2020) 134(17), 2279–2294, the discovery of a novel protective molecule against hepatic IR damage: dual-specificity phosphatase 12 (DUSP12). IR significantly decreased DUSP12 expression in liver whereas DUSP12 overexpression in hepatocytes protected IRI and DUSP12 deletion in DUSP12 KO mice exacerbated IRI. The protective effects of DUSP12 depended on apoptosis signal-regulating kinase 1 (ASK1) and acted through the inhibition of the ASK1-dependent kinases c-Jun N-terminal kinase (JNK) and p38 mitogen-activated protein kinase (MAPK). These results enlighten DUSP12 as a novel intermediate negative regulator of the pro-inflammatory and pro-apoptotic ASK1/JNK-p38 MAPK pathway activated during hepatic IR and identify DUSP12 as potential therapeutic target for IRI.
Lexical and Acoustic Characteristics of Young and Older Healthy Adults
Authors: Cho Sunghye , Nevler Naomi , Shellikeri Sanjana , Parjane Natalia , Irwin David J. , Ryant Neville , Ash Sharon , Cieri Christopher , Liberman Mark , Grossman Murray 
Published: January 2021

Purpose
This study examines the effect of age on language use with an automated analysis of digitized speech obtained from semistructured, narrative speech samples.

Method
We examined the Cookie Theft picture descriptions produced by 37 older and 76 young healthy participants. Using modern natural language processing and automatic speech recognition tools, we automatically annotated part-of-speech categories of all tokens, calculated the number of tense-inflected verbs, mean length of clause, and vocabulary diversity, and we rated nouns and verbs for five lexical features: word frequency, familiarity, concreteness, age of acquisition, and semantic ambiguity. We also segmented the speech signals into speech and silence and calculated acoustic features, such as total speech time, mean speech and pause segment durations, and pitch values.

Results
Older speakers produced significantly more fillers, pronouns, and verbs and fewer conjunctions, determiners, nouns, and prepositions than young participants. Older speakers' nouns and verbs were more familiar, more frequent (verbs only), and less ambiguous compared to those of young speakers. Older speakers produced shorter clauses with a lower vocabulary diversity than young participants. They also produced shorter speech segments and longer pauses with increased total speech time and total number of words. Lastly, we observed an interaction of age and sex in pitch ranges.

Conclusions
Our results suggest that older speakers' lexical content is less diverse, and these speakers produce shorter clauses than young participants in monologic, narrative speech. Our findings show that lexical and acoustic characteristics of semistructured speech samples can be examined with automated methods.
Measuring the Subjective Cost of Listening Effort Using a Discounting Task
Authors: McLaughlin Drew J. , Braver Todd S. , Peelle Jonathan E. 
Published: January 2021

PurposeObjective measures of listening effort have been gaining prominence, as they provide metrics to quantify the difficulty of understanding speech under a variety of circumstances. A key challenge has been to develop paradigms that enable the complementary measurement of subjective listening effort in a quantitatively precise manner. In this study, we introduce a novel decision-making paradigm to examine age-related and individual differences in subjective effort during listening.MethodOlder and younger adults were presented with spoken sentences mixed with speech-shaped noise at multiple signal-to-noise ratios (SNRs). On each trial, subjects were offered the choice between completing an easier listening trial (presented at +20 dB SNR) for a smaller monetary reward and completing a harder listening trial (presented at either +4, 0, −4, −8, or −12 dB SNR) for a greater monetary reward. By varying the amount of the reward offered for the easier option, the subjective value of performing effortful listening trials at each SNR could be assessed.ResultsOlder adults discounted the value of effortful listening to a greater degree than young adults, opting to accept less money in order to avoid more difficult SNRs. Additionally, older adults with poorer hearing and smaller working memory capacities were more likely to choose easier trials; however, in younger adults, no relationship with hearing or working memory was found. Self-reported measures of economic status did not affect these relationships.ConclusionsThese findings suggest that subjective listening effort depends on factors including, but not necessarily limited to, hearing and working memory. Additionally, this study demonstrates that economic decision-making paradigms can be a useful approach for assessing subjective listening effort and may prove beneficial in future research.
Convolutional neural network for smoke and fire semantic segmentation
Authors: Frizzi Sebastien , Bouchouicha Moez , Ginoux Jean‐Marc , Moreau Eric , Sayadi Mounir 
Published: January 2021

AbstractIn recent decades, global warming has contributed to an increase in the number and intensity of wildfires destroying millions hectares of forest areas and causing many casualties each year. Firemen must therefore have the most effective means to prevent any wildfire from breaking out and to fight the blaze before being unable to contain and extinguish it. This article will present a new network architecture based on Convolutional Neural Network to detect and locate smoke and fire. This network generates fire and smoke masks in an RGB image by segmentation. The purpose of this work is to help firemen in assessing the extent of fire or monitor an incipient fire in real time with a camera embedded in a vehicle. To train this network, a database with the corresponding images and masks has been created. Such a database will allow to compare the performances of different networks. A comparison of this network with the best segmentation networks such as U‐Net and Yuan networks has highlighted its efficiency in terms of location accuracy, reduction of false positive classifications such as clouds or haze. This architecture is also efficient in real time.
Global impact of COVID 19 on 112 Emergency System detailed for National 112 Emergency System of Romania
Authors: Bărănescu Mihail , Vasilca Ionel-Sorinel , Tabarcia Mădălina , Nen Madlena 
Published: January 2021

Research background: During COVID19 crisis, in order to limit and control the multiplication of infections with the new virus, the Romanian Government forced restrictions and established fines to those not staying in line with the rules. The rules had been applied not only to citizens but also to private companies and state authorities. Due to these legal constraints taken during the declared state of emergency and the next state of alert, the appropriate attitude effects occurred in terms of behaviour of individuals and companies.
Purpose of the article: One of the positive side effects of the measures taken during the state of emergency and the state of alert, is a decrease of non-real emergency calls in public safety answering points, small in value but important for dealing with real emergencies. Moreover, the incoming calls related to some chronic non-urgent medical affection decreases.
Methods: We will study and make comparisons in the number of calls in 112 Emergency System to analyse the quantitative impact relative to COVID period versus a previous not impacted period of time because of COVID.
Findings & Value added: The impact of the pandemic on the 112 Emergency System has been a major one. We observed the need to redefine procedures and to make operational a new resilience plan, by defining a specific calling number where people in need to report incidents specifically related to COVID. We also observed some changes in caller behaviour that occurred to those calling the emergency 112 number. However, the last not the least we have observed a real interest of people calling to the specific number dedicated to COVID with different contexts, concerns and clarifications needed to them in order to adopt a more conscious and responsible attitude.
The application of External and Mixed Idea Management Systems during COVID19 time
Authors: Mikelsone Elina , Volkova Tatjana , Spilbergs Aivars , Liela Elita 
Published: January 2021

Research background: Authors have explored that there are different idea management system application types that could be used both locally and globally. But there is the question – how organizations can benefit from these systems during COVID19?
Purpose of the article: The article aims to clarify how external and mixed web-based idea management systems could be used during COVID19 time for distance idea generation sessions, as well to solve complex problems with different idea generation sources and evaluators
Methods: Literature review (data collection: systematically data collection from scientific data bases; data analysis: content analysis). Survey of n>400 enterprises with web-based IMS experience (data collection: survey; data analysis: statistics). Case studies (data collection: web-based idea management system creator secondary data; data analysis: content analysis).
Findings & Value added: External and mixed web-based idea management systems are great tools during COVID19 time for distance idea generation sessions as well to solve serious problems with different idea generation sources and evaluators. These tools could be used by private, public organizations, as well by higher education institutions and research institutions as an innovation actor under the Triple Helix Model. By involving all these organizations in the idea management process the innovation potential is increasing.
Economic and Social Impacts of COVID 19 on National Economies from the Point of View of Economic Theory
Authors: Nový Miloš , Jarý Čestmír 
Published: January 2021

Research background: The world economy is currently affected by the devastating effects of the global COVID 19 pandemic, reaching the Great Depression of the 1930s. The economic policies of the affected countries are currently focused on short-term measures on the aggregate demand side. To this end, the growth of the public finance deficit is being prepared in the area of fiscal policy, which will ultimately deepen the indebtedness of national economies. If this economic policy is to be sustainable in the long term, measures on the aggregate demand side must be accompanied by measures on the aggregate supply side.
Purpose of the article: The purpose of this article is to determine how the current globalization processes affecting the supply and demand sides of selected national economies will be affected in the short and long term as a result of the global COVID 19 pandemic.
Methods: A description of the AS - AD model will be performed, covering both short and long periods, and then a description of the impact of globalization processes on aggregate supply and aggregate demand. This will be followed by an analysis of the expected economic and social impacts of COVID 19 on individual globalization processes affecting the supply and demand sides of selected national economies and economic units.
Findings & Value added: The article will help to find a systemic approach to the recovery of national economies affected by the effects of the global pandemic COVID 19 through the prism of the AS - AD model.