bluuebunny/crossref_metadata_2025_split huggingface dataset:
From Secretion in Pichia pastoris to Application in Apple Juice Processing: Exo-Polygalacturonase from Sporothrix schenckii 1099-18
Authors: Karataş Ersin , Tülek Ahmet , Çakar Mehmet Mervan , Tamtürk Faruk , Aktaş Fatih , Binay Barış 
Published: January 2021

Background:
Polygalacturonases are a group of enzymes under pectinolytic enzymes related to enzymes that
hydrolyse pectic substances. Polygalacturonases have been used in various industrial applications such as fruit juice
clarification, retting of plant fibers, wastewater treatment drinks fermentation, and oil extraction.

Objectives:
The study was evaluated at the heterologous expression, purification, biochemical characterization,
computational modeling, and performance in apple juice clarification of a new exo-polygalacturonase from Sporothrix
schenckii 1099-18 (SsExo-PG) in Pichia pastoris.

Methods:
Recombinant DNA technology was used in this study. Two different pPIC9K plasmids
were constructed with native signal sequence-ssexo-pg and alpha signal sequence-ssexo-pg separately.
Protein expression and purification performed after plasmids transformed into the Pichia
pastoris. Biochemical and structural analyses were performed by using pure SsExo-PG.

Results:
The purification of SsExo-PG was achieved using a Ni-NTA chromatography system. The
enzyme was found to have a molecular mass of approximately 52 kDa. SsExo-PG presented as stable
at a wide range of temperature and pH values, and to be more storage stable than other commercial
pectinolytic enzyme mixtures. Structural analysis revealed that the catalytic residues of SsExo-
PG are somewhat similar to other Exo-PGs. The KMand kcatvalues for the degradation of polygalacturonic
acid (PGA) by the purified enzyme were found to be 0.5868 μM and 179 s-1, respectively.
Cu2+ was found to enhance SsExo-PG activity while Ag2+ and Fe2+ almost completely inhibited
enzyme activity. The enzyme reduced turbidity up to 80% thus enhanced the clarification of apple
juice. SsExo-PG showed promising performance when compared with other commercial pectinolytic
enzyme mixtures.

Conclusion:
The clarification potential of SsExo-PG was revealed by comparing it with commercial
pectinolytic enzymes. The following parameters of the process of apple juice clarification processes
showed that SsExo-PG is highly stable and has a novel performance.
Gate Diffusion Input Based 10-T CNTFET Power Efficient Full Adder
Authors: Tyagi Priyanka , Singh Sanjay Kumar , Dua Piyush 
Published: January 2021

Background:
Full adder is the key element of digital electronics. The CNTFET is the
most promising device in modern electronics. To enhance the performance of the full adder, CNTFET
is used in place of the CMOS.

Objective:
To implement the high speed full adder circuit for advance applications of the digital
world.

Methods:
Full adder circuit with a new Gate diffusion technique has been implemented in this work.
This is a comparative study of the 10-T CNTFET full adder with GDI technique and the 10-T Finfet
based full adder using GDI technique. Ultra-low-power feature is the additional advantage of the
GDI technique. This technology provides the full swing voltage to the circuit. Moreover, it also reduces
the number of transistors required. This technique has been used with CNTFET to upgrade
the full adder in terms of the dissipated power and product of power consumed and delay introduced
in the circuit.

Results:
The proposed design shows that the low power dissipation comes out to be approximately
4.3nW at 0.5volts. The power delay product is 4.7x10-20 J at the same voltage level. The FinFET
design also shows the better performance with GDI. But GDI enhances CNTFET based design power
consumption by about 32% from the FinFET.

Conclusions:
CNTFET showed a better response due to good current conductivity as compared to
the FinFET. This work has been implemented and simulated on the 32nm node technology.
THE INFLUENCE OF INFORMATION SHARING AND INFORMATION QUALITY TOWARD SUPPLY CHAIN INTEGRATION AND ENTERPRISE PERFORMANCE (Study of Shallot Farmers in East Java)
Authors: Musyafi Roihatul , Utami Hamidah Nayati , Prasetya Arik 
Published: January 2021

ABSTRACTÂ Purpose â€” This research was conducted to study the effect of information sharing and information quality toward supply chain integration and farmer business performance.Design/methodology/approach â€” The type of this research is quantitative explanatory research. The data used in this study are primary data obtained by questionnaire. The sampling method uses multistage proportional random sampling, sample obtained amount 200 farmers. Data analysis in this study uses path analysis method with SPSS 23.Findings â€” The results of this research indicate that the quality of information is not significant to the integration of supply chain and farmer business performance. Information sharing has a positive and significant effect toward supply chain integration and farmer business performance, supply chain integration has a positive and significant effect toward farmer business performance.Practical Implications â€” Increasing global business competition and efforts to shorten product life cycles are the two main factors that encourage businesses to change their focus from competitive competition to mutually beneficial relationships and cooperation to improve business performance.Originality/value â€” The main objective of supply chain activities for business activities is a crucial element because with these activities the goods produced can reach the end consumers.Keywords â€” Information Sharing, Information Quality, Supply Chain Integration, Enterprise Performance.
EXPLORING SKILLS NEEDED FOR DISRUPTIVE DIGITAL BUSINESS
Authors: Handayani Dewi Wahyu , Tjakraatmadja Jann Hidajat , Ghazali Achmad 
Published: January 2021

Â Research Purpose â€“ In todayâ€™s disruptive digital business era, many new business models, such as digital start-ups, have emerged, and this phenomenon needs workers with particular skills. The aims of this preliminary empirical research paper are to explore and identify the skills needed for disruptive digital business in the Indonesian context, particularly in the Jakarta region.Design/methodology/approach â€“ This qualitative study conducted semi-structured interviews with ten respondents from various types of Indonesian start-ups, such as unicorn start-up, financial technology and education technology, that have been in operation four to 12 years. The interviews were based on five core questions with the purpose of exploring respondentsâ€™ experience regarding skills needed and challenges faced at work in disruptive digital business. Observation was conducted at the Education Technology start-up office located in Jakarta with an aim to investigate workplace environment, and triangulation was used to validate the interviewsâ€™ results.Findings â€“ The results show that (1) innovativeness, (2) leadership skills, (3) social interaction, (4) initiative mindsets, (5) self-disruption, (6) critical thinking, (7) management, and (8) analytical thinking are eight pivotal skills identified for managing disruptive digital business.Practical implications â€“ Innovativeness, leadership and social interaction are the top three skills that are essential for actors in Indonesian digital start-ups to have competitive advantages in this disruptive digital business era.Original/value â€“ This paper explores skills needed for the disruptive digital era in an Indonesian context.Keywords â€“ skills concepts, disruptive, digital businessPaper type â€“ Research paper
De Coupure in Gent Scheiding en Verbinding.
Authors: Brysse Adrien 
Published: January 2021

Op initiatief van prof. em. dr. Marcel Storme zag in 2005 een werkgroep hetlevenslicht om het verleden van de Coupure in Gent in kaart te brengen. Het boek is opgedeeld volgens de grote historische breuklijnen.
Slachtoffer van communistische terreur? De moord op onderwijzer Herman De Vos, 13 september 1944
Authors: Meerpoel Robbe 
Published: January 2021

Op 13 september 1944 werd de Aalsterse onderwijzer en medeoprichter van de Vlaamsche Kinderzegen Herman De Vos door leden van het verzet neergeschoten. Het was pas wanneer deze verzetsgroep in mei 1945 nog een tweede moord pleegde dat een onderzoek werd geopend. Tijdens het proces dat in 1950 aan het Gentse assisenhof werd gevoerd, werden de daders in de pers voorgesteld als een groep ontspoorde verzetsleden die verbonden waren met de lokale communistische partij. Herman De Vos werd afgebeeld als een willekeurig slachtoffer van het bevrijdingsgeweld. De casus werd later door voormalige collaborateurs gebruikt om het verzet in diskrediet te brengen en de framing van een repressie ‘zonder maat of einde’ kracht bij te zetten. Deze beeldvorming werd dominant in de herinnering aan de bevrijding in Aalst, doordat historici zich op tijdens het proces verschenen krantenartikelen baseerden om de moord te reconstrueren. Onderzoek van de procesdossiers bracht echter nieuwe elementen over de rol van de verschillende verzetsorganisaties en de lokale communistische partij aan het licht. De hoofdverantwoordelijke voor de moord gebruikte communisme als dekmantel om het geweld te rechtvaardigen. Herman De Vos was een toevallig slachtoffer omdat het eigenlijke doelwit, VTB-ondervoorzitter Jozef Van Overstraeten onvindbaar was. De opdracht om hem te vermoorden werd wel degelijk gedekt door het Aalsterse verzet._________
A victim of communist terror?The murder on the teacher Herman De Vos, September 13, 1944
On September 13, 1944, the teacher and co-founder of the ‘Vlaamsche Kinderzegen’ [Flemish Child Blessing], Herman De Vos, was shot in Aalst by members of the resistance. An investigation however would only be initiated after this resistance movement had committed a second murder in May 1945. During the trial – taking place at the court of assize in Ghent in 1950 – the press depicted the culprits as a group of deranged members of the resistance that were associated with the local communist party. Conversely, De Vos was portrayed as an incidental victim of the violence that ensued after the liberation of Belgium, later granting former collaborators a case to discredit the resistance, and enhance their framing of the repression as being ‘without rule or resolution.’ Moreover, this portrayal has become ubiquitous in the memory of the liberation of Aalst as historians have mainly focused on contemporaneous newspaper articles to reconstruct the trial. Analysis of the trial transcripts and documents however sheds a new light on the role of the different resistance movements and local communist party. The main culprit of the murder used communism as a pretext to justify the violence. In addition, De Vos was an unintended victim because they could not locate their actual target, Jozef Van Overstraeten, vice-chairman of the VTB [Flemish Tourist Association]. The order to murder Van Overstraeten had, in fact, been supported by the resistance in Aalst.
"Natuer en kunst gaen hier hand aen hand". Duitslandbeelden in de vroege Vlaamse beweging (1844-1848)
Authors: Langhendries Maarten 
Published: January 2021

In de jaren 1840 cultiveerde de jonge Vlaamse beweging een sterke band met de Duitse cultuur. Deze bijdrage belicht welk beeld de flaminganten in deze periode hadden van de Duitse gebieden en van de Duitstalige cultuur, met name de Duitse literatuur. Een analyse van de Duitsgezinde tijdschriften De Broederhand en Het Taelverbond toont dat er onder de Duitsgezinde flaminganten een sterk geïdealiseerd beeld leefde van een groen en ruraal Duitsland. Het Duitsland dat in de twee periodieken geschetst werd, is een land van ‘dichters en denkers’, waar muziek en literatuur hun ‘ware’ volkse karakter hebben weten te behouden. Dit authentieke karakter maakte de Duitse cultuur superieur aan met name de Franse. Vlaanderen diende zich volgens de twee tijdschriften te spiegelen aan haar oosterburen in het levend en ‘oorspronkelijk’ houden van haar taal en geschiedenis.__________
“Here, nature and culture walk hand in hand”. Images of German culture in the early Flemish movement (1844-1848).
In the 1840s, the Flemish movement cultivated a strong connection to the German culture. This paper discusses the image of the German territories and German culture, in particular German literature, amongst the Flemish nationalists. An analyses of the pro-German periodicals De Broederhand and Het Taelverbond shows that these nationalists had a very romantic image of Germany as rural and characterized by unspoiled nature. The two periodicals sketch an image of a country where everybody is a poet or a philosopher. In the eyes of the Flemish nationalists, German music and literature managed to keep its ‘true’ folksy character. The authenticity of German culture made it superior to in particular French culture. According to the two magazines, Flanders has to follow the example of the Germans in keeping alive its language and past.
Profeten, psalmisten en onruststokers. Hans Kohn (1891-1971) over het Vlaamse nationalisme
Authors: Swerts Kasper 
Published: January 2021

Dit artikel werpt nieuw licht op het leven en werk van de Praagse historicus Hans Kohn (1891-1971) en zijn studie over het nationalisme in de Lage Landen, in het bijzonder het Vlaams nationalisme. Het traceert de invloed van de zionistische filosoof Martin Buber (1878-1965) op Kohns studie van het nationalisme. Buber onderscheidde drie ideaaltypes – profeten, psalmisten en koningen – die Kohn zou hanteren om zijn vergelijkende studies van het nationalisme te ondersteunen. Kohn paste deze types ook toe op de Vlaamse casus, wat tot een opvallende interpretatie van het Vlaams nationalisme, en de Vlaamse beweging in haar geheel zou leiden. Kohn stelde immers dat het Vlaams nationalisme een succesvolle nationale beweging was omdat ze streefde naar een harmonie in het Belgische staatsbestel. Via de drie ideaaltypes belichtte Kohn voornamelijk dichters, schrijvers en politici die ijverden voor harmonie en eenheid, en bestempelde nationalisten die pleitten voor een onafhankelijke Vlaamse natie als nefaste onruststokers. Ondanks de duidelijke tekortkomingen in Kohns analyse van de Vlaamse casus, noopt het werk van de Praagse historicus ons echter nog steeds tot reflectie over het nationalisme, en blijft hij ook nu nog relevant voor het onderzoek naar het nationalisme, zowel in Vlaanderen als elders._________
Prophets, Psalmists and Agitators. Hans Kohn (1891-1971) on Flemish nationalism
This article shines new light on the life and work of the Prague historian Hans Kohn (1891-1971), on his study of nationalism in the Low Countries, and in particular on Flemish nationalism. The article traces the influence of the Zionist philosopher Martin Buber (1878-1965) on Kohn’s analysis of nationalism. Buber discerned three ideal types – prophets, psalmists and kings – which Kohn would rely upon when studying nationalism from a comparative perspective. Kohn would use these types when analysing the Flemish case, resulting in a peculiar interpretation of Flemish nationalism and the Flemish movement in general. Kohn argued that Flemish nationalism constituted a successful national movement precisely because it vied for harmony with the Belgian state. Via the three ideal types Kohn primarily highlighted poets, writers and politicians who strove for unity and harmony, whilst simultaneously branding nationalists that pursued an independent Flemish nation as pernicious agitators. While Kohn’s analysis did contain clear shortcomings, the Prague historian’s peculiar analysis of the Flemish case still compels us to reflect on nationalism, highlighting Kohn’s continued relevance for the study of nationalism, in Flanders and elsewhere.
Effect of Interactive eHealth Interventions on Improving Medication Adherence in Adults With Long-Term Medication: Systematic Review
Authors: Pouls Bart P H , Vriezekolk Johanna E , Bekker Charlotte L , Linn Annemiek J , van Onzenoort Hein A W , Vervloet Marcia , van Dulmen Sandra , van den Bemt Bart J F 
Published: January 2021

Background
Medication nonadherence leads to suboptimal treatment outcomes, making it a major priority in health care. eHealth provides an opportunity to offer medication adherence interventions with minimal effort from health care providers whose time and resources are limited.

Objective
The aim of this systematic review is twofold: (1) to evaluate effectiveness of recently developed and tested interactive eHealth (including mHealth) interventions on medication adherence in adult patients using long-term medication and (2) to describe strategies among effective interventions.

Methods
MEDLINE, EMBASE, Cochrane Library, PsycINFO, and Web of Science were systematically searched from January 2014 to July 2019 as well as reference lists and citations of included articles. Eligible studies fulfilled the following inclusion criteria: (1) randomized controlled trial with a usual care control group; (2) a total sample size of at least 50 adult patients using long-term medication; (3) applying an interactive eHealth intervention aimed at the patient or patient’s caregiver; and (4) medication adherence as primary outcome. Methodologic quality was assessed using the Cochrane risk of bias tool. Selection and quality assessment of studies were performed by 2 researchers (BP and BvdB or JV) independently. A best evidence synthesis was performed according to the Cochrane Back Review Group.

Results
Of the 9047 records screened, 22 randomized clinical trials were included reporting on 29 interventions. Most (21/29, 72%) interventions specified using a (mobile) phone for calling, SMS text messaging, or mobile apps. A majority of all interactive interventions (17/29) had a statistically significant effect on medication adherence (P<.05). Of these interventions, 9 had at least a small effect size (Cohen d ≥ 0.2) and 3 showed strong odds for becoming adherent in the intervention group (odds ratio > 2.0). Our best evidence synthesis provided strong evidence for a positive effect of interventions using SMS text messages or interactive voice response, mobile app, and calls as mode of providing adherence tele-feedback. Intervention strategies “to teach medication management skills,” “to improve health care quality by coordinating medication adherence care between professionals,” and “to facilitate communication or decision making between patients and health care providers” also showed strong evidence for a positive effect.

Conclusions
Overall, this review supports the hypothesis that interactive eHealth interventions can be effective in improving medication adherence. Intervention strategies that improve patients’ treatment involvement and their medication management skills are most promising and should be considered for implementation in practice.
Effectiveness of the Alfalfa App in Warfarin Therapy Management for Patients Undergoing Venous Thrombosis Prevention and Treatment: Cohort Study
Authors: Cao Hua , Jiang Shaojun , Lv Meina , Wu Tingting , Chen Wenjun , Zhang Jinhua 
Published: January 2021

Background
Over the years, the internet has enabled considerable progress in the management of chronic diseases, especially hypertension and diabetes. It also provides novel opportunities in online anticoagulation management. Nevertheless, there is insufficient evidence regarding the effectiveness of online anticoagulation management.

Objective
This study explored the effectiveness and safety of warfarin management via the Alfalfa app, so as to provide evidence in support of anticoagulant management through online services.

Methods
In this retrospective, observational cohort study, 824 patients were included. In the offline group, patients went to the hospital clinic for warfarin management. In the Alfalfa app group, patients reported the dose of warfarin, current international normalized ratio (INR) value, and other related information through the Alfalfa app. Physicians or pharmacists used the app to adjust the dose of warfarin and determined the time for the next blood INR testing. Patients completed INR testing by point-of-care at home or hospital. The primary outcome of the study was the percentage of time in therapeutic range (TTR). Secondary outcomes included minor and major bleeding events, thrombotic events, warfarin-related emergency department visits, hospital admissions, and high INR values.

Results
The TTR and percentage of INR values in the range were significantly higher in the Alfalfa app group than in the offline group (79.35% vs 52.38%, P<.001; 3314/4282, 77.39% vs 2005/4202, 47.72%, P<.001, respectively). Patients managed via the Alfalfa app had lower rates of subtherapeutic (172/4282, 4.02% vs 388/4202, 9.23%; P<.001), supratherapeutic (487/4282, 11.37% vs 882/4202, 20.99%; P<.001), and extreme subtherapeutic INR values (290/4282, 6.77% vs 910/4202, 21.66%; P<.001). Additionally, the Alfalfa app group had lower incidences of major bleeding (2/425, 0.5% vs 12/399, 3.0%; P=.005), warfarin-related emergency department visits (13/425, 3.1% vs 37/399, 9.3%; P<.001), and hospital admissions (1/425, 0.2% vs 12/399, 3.0%; P=.001) compared with the offline group. However, the Alfalfa app group had a higher incidence of minor bleeding than the offline group (45/425, 10.6% vs 20/399, 5.0%; P=.003). There were similar incidences in extreme supratherapeutic INR values (19/4282, 0.44% vs 17/4202, 0.40%; P=.78) and thromboembolic events (1/425, 0.2% vs 1/399, 0.3%; P=.53) between the two groups.

Conclusions
Warfarin management is superior via the Alfalfa app than via offline services in terms of major bleeding events, warfarin-related emergency department visits, and hospital admissions.
HOLDING RESPONSIBLE RECONSIDERED
Authors: Svirsky Larisa 
Published: January 2021

Abstract
Following Strawson, many philosophers have claimed that holding someone responsible necessitates its being appropriate to feel or express the negative reactive attitudes (e.g., resentment, indignation, and anger) toward her. This view, while compelling, is unable to capture the full range of cases in which we hold others responsible in ordinary life. Consider the parent who holds her 5-year-old responsible for not teasing his sister, or the therapist who holds her patient responsible for avoiding self-injurious behavior. Holding responsible in such cases requires enforcing normative expectations, but these norms can (and typically should) be enforced without involving the negative reactive attitudes. To demonstrate this, I consider how responsibility attributions function in psychotherapy, as well as in other contexts where the negative reactive attitudes do not have a natural home.