bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mitigating imperfect data validity in administrative data PSIs: a method for estimating true adverse event rates
Authors: Boussat Bastien , Quan Hude , Labarere Jose , Southern Danielle , Couris Chantal M , Ghali William A 
Published: February 2021

Abstract

Question
Are there ways to mitigate the challenges associated with imperfect data validity in Patient Safety Indicator (PSI) report cards?

Findings
Applying a methodological framework on simulated PSI report card data, we compare the adjusted PSI rates of three hospitals with variable quality of data and coding. This framework combines (i) a measure of PSI rates using existing algorithms; (ii) a medical record review on a small random sample of charts to produce a measure of hospital-specific data validity and (iii) a simple Bayesian calculation to derive estimated true PSI rates. For example, the estimated true PSI rate, for a theoretical hospital with a moderately good quality of coding, could be three times as high as the measured rate (for example, 1.4% rather than 0.5%). For a theoretical hospital with relatively poor quality of coding, the difference could be 50-fold (for example, 5.0% rather than 0.1%).

Meaning
Combining a medical chart review on a limited number of medical charts at the hospital level creates an approach to producing health system report cards with estimates of true hospital-level adverse event rates.
AUCs and 123s: a critical appraisal of vancomycin therapeutic drug monitoring in paediatrics
Authors: Jorgensen Sarah C. J. , Dersch-Mills Deonne , Timberlake Kathryn , Stewart Jackson J. , Gin Alfred , Dresser Linda D. , Dalton Bruce R. 
Published: February 2021

AbstractThe revised vancomycin guidelines recommend implementing AUC24-based therapeutic drug monitoring (TDM) using Bayesian methods in both adults and paediatrics. The motivation for this change was accumulating evidence showing aggressive dosing to achieve high troughs, as recommended in the first guidelines for adults and extrapolated to paediatrics, is associated with increased nephrotoxicity without improving clinical outcomes. AUC24-based TDM requires substantial resources that may need to be diverted from other valuable interventions. It can therefore be justified only after certain assumptions are shown to be true: (i) there is a clear relationship between vancomycin efficacy and/or toxicity and the proposed therapeutic range; and (ii) maintaining exposure within the target range with AUC24-based TDM improves clinical outcomes and/or decreases toxicity. In this review, we critically appraise the scientific basis for these assumptions. We find studies evaluating the relationship between vancomycin AUC24/MIC and efficacy in adults and children do not offer strong support for the recommended lower limit of the proposed therapeutic range (i.e. AUC24/MIC ≥400). Nephrotoxicity in children increases in a stepwise manner along the vancomycin exposure continuum but it is unclear if one parameter (AUC24 versus trough) is a superior predictor. Overall, evidence in children suggests good-to-excellent correlation between AUC24 and trough. Most importantly, there is no convincing evidence that the method of vancomycin TDM has a causal role in improving efficacy or reducing toxicity. These findings question the need to transition to resource-intensive AUC24-based TDM over retaining trough-based TDM with lower targets to minimize nephrotoxicity in paediatrics.
Complete Fusion of Both Eyelids in Stevens-Johnson Syndrome: Case Report
Authors: Santamaria Joseph A , Cancio Leopoldo C , Reed Donovan , Phillips Hunter , Chen Sien , Carlton Darrel K , Johnson Anthony J 
Published: February 2021

Abstract
An 18-year-old woman developed Stevens-Johnson syndrome (SJS) with ocular involvement after taking ibuprofen. She was admitted to another hospital, received saline flushes and bacitracin ophthalmic ointment to the eyes, and became unable to open them. Upon transfer to this burn center 3 weeks after symptom onset, there was complete fusion of both eyelids with no visible cornea or sclera. She underwent bilateral operative scar release. After opening the lids, meticulous debridement of cicatricial membranes and release of symblephara were performed with subsequent placement of amniotic membrane grafts. Her vision slowly improved, though her long-term visual prognosis remains guarded. Early recognition and treatment of SJS or toxic epidermal necrolysis (TEN) with ocular involvement is imperative. Even mild cases may require intensive topical lubrication, steroids, and antibiotics, with early placement of amniotic membrane grafts in severe cases. Prompt intervention and daily evaluation are paramount in preventing lifelong visual disability.
Historical Origins of the Personal Belief Exemption to Vaccination Mandates: The View from California
Authors: Conis Elena , Kuo Jonathan 
Published: February 2021

Abstract
A number of states, starting with California, have recently removed all non-medical exemptions from their laws requiring vaccinations for schoolchildren. California was also one of the earliest states to include a broad non-medical, or personal, belief exemption in its modern immunization law, which it did with a 1961 law mandating polio vaccination for school enrollment, Assembly Bill 1940 (AB 1940). This paper examines the history of AB 1940’s exemption clause as a case study for shedding light on the little-examined history of the personal belief exemption to vaccination in the United States. This history shows that secular belief exemptions date back further than scholars have allowed. It demonstrates that such exemptions resulted from political negotiation critical to ensuring compulsory vaccination’s political success. It challenges a historiography in which antivaccination groups and their allies led late-nineteenth and early-twentieth century opposition to vaccination mandates while religious groups drove mid-twentieth century opposition. It also complicates the historiographic idea of a return to compulsion in the late 1960s, instead dating this return a decade earlier, to a time when belief exemptions in polio vaccination mandates helped reconcile the goal of a widely vaccinated population with the sacrosanct idea of health as a personal responsibility.
Progress in heterologous biosynthesis of forskolin
Authors: Ju Haiyan , Zhang Chuanbo , Lu Wenyu 
Published: February 2021

Abstract
Forskolin, a class of labdane-type diterpenoid, has significant medicinal value in anticancer, antiasthmatic, antihypertensive, and heart-strengthening treatments. The main source of natural forskolin is its extraction from the cork tissue of the root of Coleus forskohlii. However, conventional modes of extraction pose several challenges. In recent years, the construction of microbial cell factories to produce medicinal natural products via synthetic biological methods has effectively solved the current problems and is a research hotspot in this field. This review summarizes the recent progress in the heterologous synthesis of forskolin via synthetic biological technology, analyzes the current challenges, and proposes corresponding strategies.
Lumbar and sacral brucellosis due to queso fresco ingestion
Authors: Krause Cassandra , Chang Michael , Veltman Jennifer , Luo-Owen Xian , Kim Esther , Danisa Olumide , Abou-Zamzam Ahmed M , Mukherjee Kaushik 
Published: February 2021

Abstract
Brucellosis is rare in the USA, with 100–200 cases reported annually. In this case we illustrate the complex management of spinal brucellosis. A 22-year-old male presented with 3 months of low back pain. Imaging revealed osteomyelitis/diskitis of L5/S1 and abscesses involving the pre-vertebral, epidural and pelvic recesses. Initial biopsies were inconclusive, but the patient later endorsed eating unpasteurized cheese (queso fresco) from Mexico; therefore, Brucella serology was sent and was positive. Despite aggressive antibiotic therapy there was disease progression. The patient underwent debridement of the involved vertebrae and drainage. Multiple cultures failed to grow the organisms, but Brucella polymerase chain reaction was positive. A month later he underwent a second vertebral debridement as well as placement of tobramycin impregnated beads in the vertebral space. He has since recovered. Surgery should be considered if there is a poor response to medical management and patients may need repeated debridement.
Bilateral delayed traumatic diaphragmatic injury
Authors: Sauer Durand Anna M , Nebiker Christian A , Hartel Mark , Kremer Michael 
Published: February 2021

ABSTRACT
A 47-year-old patient presented at our emergency department with acute epigastric pain. A thoracic X-ray showed a partially intrathoracic stomach as well as bowel left sided. A following computed tomography scan diagnosed a diaphragmatic hernia. In the patient’s history, 20 years ago a serious car accident was reported as the presumable traumatic origin. Intraoperatively, the diaphragmatic hernia was repaired with a direct suture and mesh augmentation. The rest of the abdomen was clear. In a thoracic X-ray following chest tube removal, herniated small bowel appeared intrathoracally on the right. Relaparotomy showed an extensive diaphragmatic hernia with parts of the liver, small bowel and colon in the right thoracic cavity. Only a partial direct repair was possible, an inlay mesh repair was performed. The further recovery was uneventful.
Bilateral delayed traumatic diaphragmatic hernias are extremely rare, but with a suggestive trauma history thorough intraoperative exploration of the contralateral side should be evaluated.
Coronary artery aneurysm complicated by pneumothorax with a history of Kawasaki disease
Authors: Ishida Shinichi , Mutsuga Masato , Fujita Takashi , Ito Masao , Okamoto Sawako , Yagami Kei 
Published: February 2021

Abstract
Kawasaki disease (KD) is a common vasculitis disorder of childhood. It can sometimes complicate coronary artery aneurysms, and treatment is required depending on the condition of stenosis. A 20-year-old man was referred for surgery with a coronary artery aneurysm and stenosis in the left coronary artery as sequelae of KD. He had a surgical history of left pneumothorax and bullae remaining on the right lung. We simultaneously performed off-pump coronary artery bypass for coronary artery stenosis and bullectomy. Coronary artery aneurysms with KD complicated by pneumothorax are rare, and we treated them using one-stage surgery.
Discovery of five new Galactic symbiotic stars in the VPHAS+ survey
Authors: Akras Stavros , Gonçalves Denise R , Alvarez-Candal Alvaro , Pereira Claudio B 
Published: February 2021

ABSTRACT
We report the validation of a recently proposed infrared (IR) selection criterion for symbiotic stars (SySts). Spectroscopic data were obtained for seven candidates, selected from the SySt candidates of Akras et al. by employing the new supplementary IR selection criterion for SySts in the VST/OmegaCAM Photometric H-Alpha Survey. Five of them turned out to be genuine SySts after the detection of H α, He ii, and [O iii] emission lines as well as TiO molecular bands. The characteristic O vi Raman-scattered line is also detected in one of these SySts. According to their IR colours and optical spectra, all five newly discovered SySts are classified as S-type. The high rate of true SySts detections of this work demonstrates that the combination of the H α emission and the new IR criterion improves the selection of target lists for follow-up observations by minimizing the number of contaminants and optimizing the observing time.
Dispersion measure distributions of fast radio bursts due to the intergalactic medium
Authors: Medlock Isabel , Cen Renyue 
Published: February 2021

ABSTRACT
Utilizing cosmological hydrodynamic simulations, we quantify the distributions of the dispersion measure (DM) of fast radio bursts (FRBs). We examine the contributions of cold, warm-hot, and hot gas to the total DM. We find that the hot gas component (T &gt; 107K), on average, makes a minor contribution ($\le 5{{\ \rm per\ cent}}$) to the overall DM. Cold (T &lt; 105K) and warm-hot (T = 105 − 107K) gas components make comparable contributions to DM for FRBs at z = 1, with the former component making an increasingly larger contribution towards higher redshift. We provide a detailed DM distribution of FRBs at z = 0.25 to z = 2 that may be compared to observations. We also compute the relation between the Compton y parameter and DM, finding a strong correlation, y∝DM4, providing an additional, independent constraint on the nature of the DM of FRBs.
Formation and evolution of protostellar accretion discs – I. Angular-momentum budget, gravitational self-regulation, and numerical convergence
Authors: Xu Wenrui , Kunz Matthew W 
Published: February 2021

ABSTRACT
We investigate the formation and early evolution of a protostellar disc from a magnetized prestellar core using non-ideal magnetohydrodynamic (MHD) simulations including ambipolar diffusion and Ohmic dissipation. The dynamical contraction of the prestellar core ultimately leads to the formation of a first hydrostatic core, after ambipolar diffusion decouples the magnetic field from the predominantly neutral gas. The hydrostatic core accumulates angular momentum from the infalling material, evolving into a rotationally supported torus; this ‘first hydrostatic torus’ then forms an accreting protostar and a rotationally supported disc. The disc spreads out by gravitational instability, reaching ∼30 au in diameter at ∼3 kyr after protostar formation. The total mass and angular momentum of the protostar–disc system are determined mainly by accretion of gas from an infalling pseudo-disc, which has low specific angular momentum because of magnetic braking; their removal from the protostar–disc system by outflow and disc magnetic braking are negligible, in part because the magnetic field is poorly coupled there. The redistribution of angular momentum within the protostar–disc system is facilitated mainly by gravitational instability; this allows formation of relatively large discs even when the specific angular momentum of infalling material is low. We argue that such discs should remain marginally unstable as they grow (with Toomre Q ∼ 1–2), an idea that is broadly consistent with recent observational estimates for Class 0/I discs. We discuss the numerical convergence of our results, and show that properly treating the inner boundary condition is crucial for achieving convergence at an acceptable computational cost.
Quantiles as robust probes of non-Gaussianity in 21-cm images
Authors: Banet Alon , Barkana Rennan , Fialkov Anastasia , Guttman Or 
Published: February 2021

ABSTRACT
The epoch in which the first stars and galaxies formed is among the most exciting unexplored eras of the Universe. A major research effort is focused on probing this era with the 21-cm spectral line of hydrogen. While most research works focus on statistics like the 21-cm power spectrum or the sky-averaged global signal, there are other ways to analyse tomographic 21-cm maps, which may lead to novel insights. We suggest statistics based on quantiles as a method to probe non-Gaussianities of the 21-cm signal. We show that they can be used in particular to probe the variance, skewness, and kurtosis of the temperature distribution, but are more flexible and robust than these standard statistics. We test these statistics on a range of possible astrophysical models, including different galactic halo masses, star formation efficiencies, and spectra of the X-ray heating sources, plus an exotic model with an excess early radio background. Simulating data with angular resolution and thermal noise as expected for the Square Kilometre Array (SKA), we conclude that these statistics can be measured out to redshifts above 20 and offer a promising statistical method for probing early cosmic history.
Informative Bayesian model selection for RR Lyrae star classifiers
Authors: Pérez-Galarce F , Pichara K , Huijse P , Catelan M , Mery D 
Published: February 2021

ABSTRACT
Machine learning has achieved an important role in the automatic classification of variable stars, and several classifiers have been proposed over the last decade. These classifiers have achieved impressive performance in several astronomical catalogues. However, some scientific articles have also shown that the training data therein contain multiple sources of bias. Hence, the performance of those classifiers on objects not belonging to the training data is uncertain, potentially resulting in the selection of incorrect models. Besides, it gives rise to the deployment of misleading classifiers. An example of the latter is the creation of open-source labelled catalogues with biased predictions. In this paper, we develop a method based on an informative marginal likelihood to evaluate variable star classifiers. We collect deterministic rules that are based on physical descriptors of RR Lyrae stars, and then, to mitigate the biases, we introduce those rules into the marginal likelihood estimation. We perform experiments with a set of Bayesian logistic regressions, which are trained to classify RR Lyraes, and we found that our method outperforms traditional non-informative cross-validation strategies, even when penalized models are assessed. Our methodology provides a more rigorous alternative to assess machine learning models using astronomical knowledge. From this approach, applications to other classes of variable stars and algorithmic improvements can be developed.
Gv1, a Zinc Finger Gene Controlling Endogenous MLV Expression
Authors: Young George R , Ferron Aaron K W , Panova Veera , Eksmond Urszula , Oliver Peter L , Kassiotis George , Stoye Jonathan P 
Published: February 2021

Abstract
The genomes of inbred mice harbor around 50 endogenous murine leukemia virus (MLV) loci, although the specific complement varies greatly between strains. The Gv1 locus is known to control the transcription of endogenous MLVs and to be the dominant determinant of cell-surface presentation of MLV envelope, the GIX antigen. Here, we identify a single Krüppel-associated box zinc finger protein (ZFP) gene, Zfp998, as Gv1 and show it to be necessary and sufficient to determine the GIX+ phenotype. By long-read sequencing of bacterial artificial chromosome clones from 129 mice, the prototypic GIX+ strain, we reveal the source of sufficiency and deficiency as splice-acceptor variations and highlight the varying origins of the chromosomal region encompassing Gv1. Zfp998 becomes the second identified ZFP gene responsible for epigenetic suppression of endogenous MLVs in mice and further highlights the prominent role of this gene family in control of endogenous retroviruses.
N
6-Methyladenosines in mRNAs reduce the accuracy of codon reading by transfer RNAs and peptide release factors
Authors: Ieong Ka-Weng , Indrisiunaite Gabriele , Prabhakar Arjun , Puglisi Joseph D , Ehrenberg Måns 
Published: February 2021

Abstract
We used quench flow to study how N6-methylated adenosines (m6A) affect the accuracy ratio between kcat/Km (i.e. association rate constant (ka) times probability (Pp) of product formation after enzyme-substrate complex formation) for cognate and near-cognate substrate for mRNA reading by tRNAs and peptide release factors 1 and 2 (RFs) during translation with purified Escherichia coli components. We estimated kcat/Km for Glu-tRNAGlu, EF-Tu and GTP forming ternary complex (T3) reading cognate (GAA and Gm6AA) or near-cognate (GAU and Gm6AU) codons. ka decreased 10-fold by m6A introduction in cognate and near-cognate cases alike, while Pp for peptidyl transfer remained unaltered in cognate but increased 10-fold in near-cognate case leading to 10-fold amino acid substitution error increase. We estimated kcat/Km for ester bond hydrolysis of P-site bound peptidyl-tRNA by RF2 reading cognate (UAA and Um6AA) and near-cognate (UAG and Um6AG) stop codons to decrease 6-fold or 3-fold by m6A introduction, respectively. This 6-fold effect on UAA reading was also observed in a single-molecule termination assay. Thus, m6A reduces both sense and stop codon reading accuracy by decreasing cognate significantly more than near-cognate kcat/Km, in contrast to most error inducing agents and mutations, which increase near-cognate at unaltered cognate kcat/Km.
Blood-brain barrier alterations in human brain tumors revealed by genome-wide transcriptomic profiling
Authors: Schaffenrath Johanna , Wyss Tania , He Liqun , Rushing Elisabeth Jane , Delorenzi Mauro , Vasella Flavio , Regli Luca , Neidert Marian Christoph , Keller Annika 
Published: February 2021

Abstract

Background
Brain tumors, whether primary or secondary, have limited therapeutic options despite advances in understanding driver gene mutations and heterogeneity within tumor cells. The cellular and molecular composition of brain tumor stroma, an important modifier of tumor growth, has been less investigated to date. Only few studies have focused on the vasculature of human brain tumors despite the fact that the blood-brain barrier (BBB) represents the major obstacle for efficient drug delivery.

Methods
In this study, we employed RNA sequencing to characterize transcriptional alterations of endothelial cells (EC) isolated from primary and secondary human brain tumors. We used an immunoprecipitation approach to enrich for EC from normal brain, glioblastoma (GBM), and lung cancer brain metastasis (BM).

Results
Analysis of the endothelial transcriptome showed deregulation of genes implicated in cell proliferation, angiogenesis, and deposition of extracellular matrix (ECM) in the vasculature of GBM and BM. Deregulation of genes defining the BBB dysfunction module was found in both tumor types. We identified deregulated expression of genes in vessel-associated fibroblasts in GBM.

Conclusion
We characterize alterations in BBB genes in GBM and BM vasculature and identify proteins that might be exploited for developing drug delivery platforms. In addition, our analysis on vessel-associated fibroblasts in GBM shows that the cellular composition of brain tumor stroma merits further investigation.
Photochemistry of Photosystems II and I in Rice Plants Grown under Different N Levels at Normal and High Temperature
Authors: Takahashi Yuki , Wada Shinya , Noguchi Ko , Miyake Chikahiro , Makino Amane , Suzuki Yuji 
Published: February 2021

AbstractAlthough N levels affect leaf photosynthetic capacity, the effects of N levels on the photochemistry of photosystems II and I (PSII and PSI, respectively) are not well-understood. In the present study, we examined this aspect in rice (Oryza sativa L. ‘Hitomebore’) plants grown under three different N levels at normal or high temperatures that can occur during rice culture and do not severely suppress photosynthesis. At both growth temperatures, the quantum efficiency of PSII [Y(II)] and the fraction of the primary quinone electron acceptor in its oxidized state were positively correlated with the amount of total leaf-N, whereas the quantum yields of non-photochemical quenching and donor-side limitation of PSI [Y(ND)] were negatively correlated with the amount of total leaf-N. These changes in PSII and PSI parameters were strongly correlated with each other. Growth temperatures scarcely affected these relationships. These results suggest that the photochemistry of PSII and PSI is coordinately regulated primarily depending on the amount of total leaf-N. When excess light energy occurs in low N-acclimated plants, oxidation of the reaction center chlorophyll of PSI is thought to be stimulated to protect PSI from excess light energy. It is also suggested that PSII and PSI normally operate at high temperature used in the present study. In addition, as the relationships between Y(II) and Y(ND) were found to be almost identical to those observed in osmotically stressed rice plants, common regulation is thought to be operative when excess light energy occurs due to different causes.