bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluation parameters of in vitro morphogenesis in the process of replication of potato starting material
Authors: Oves E , Anikina I , Gaitova N , Adamzhanova Zh , Kaynidenov N 
Published: March 2021

Abstract
In the process of replicating and growing the necessary volumes of in vitro material for original seed production, the period of formation of morphological structures and the achievement of microplants of standard characteristics is important. The existing method of regeneration assessment by the quantity of days from the moment of placement of micro stalks into a new nutrient medium does not allow a planned approach to the compilation and implementation of the clonal micropropagation program. The article proposes new methodological approaches to assessing in vitro potato material by the main phases of plant development in tissue culture: intensive growth (formation of 2-3 internodes), slow growth (4-6 internodes), and natural dying-off. The analysis of regenerative ability allows us to note that the effectiveness of the formation of morphological structures did not depend on the ripeness group of the studied variety samples. Presented results indicate the differentiated approach of varieties to the formation of morphological structures in the process of replication.
Effect of genetic and paratypical factors on milk production in cattle
Authors: Lihodeevskaya O E , Lihodeevskiy G A , Gorelik O V , Loretts O G , Stepanova V V 
Published: March 2021

Abstract
The influence of genetic and paratypical factors on milk productivity has been studied for many years, but the influence of genotypes was considered as belonging to a genealogical line, which does not give correct results in terms of genetics, the influence of homo- and heterozygosity. The relevance of the work lies in the fact that for the first time a genome-wide study was carried out on the breeding stock of cattle in the Sverdlovsk region and the relationships between the influence of some genes and the paratype on milk productivity were established. In order to maximally reveal the influence of genotypes, farms with the same technological conditions, but giving a significant difference in milk productivity and quality, were selected. Analysis of complete genotypes by the method of principal components showed the genetic homogeneity of the sample, which forms a large general cluster, corresponding to the general trend of Holsteinization of black-and-white cattle. The influence of the environmental factor on productivity indicators turned out to be significant, with the exception of the proportion of protein in the third lactation, all of them were statistically significantly different P <0.01. Six genes were selected for this study: diacylglycerol-O-acyltransferase 1, leptin, growth hormone receptor gene, β-lactoglobulin, k-casein AB and CE. A high coefficient of homozygosity was revealed for two genes CSN_AB and CSN_CE (0.705 and 0.897, respectively), which may lead in the near future to purely linear animals in herds. Despite the fact that no significant difference in the influence of the genotype on productive qualities was revealed for most indicators, the actual difference in them for agricultural organizations
Accumulation of phenolic antioxidants in flowers and fruits of guelder rose (Viburnum opulus L.) depending on site conditions
Authors: Skrypnik L N , Kislyakova L A , Maslennikov P V , Feduraev P V 
Published: March 2021

Abstract
Various parts of guelder rose (Viburnum opulus L.), including the bark, leaves, flowers, and fruits, have long been used in modern and folk medicine. This study aims to evaluate how site climatic conditions and soil properties influence the accumulation of phenolic antioxidants in flowers and fruits of Viburnum opulus L. It was established that the content of total phenolics in fruits was 1.7 times that in the flowers; the content of tannins, 2.5 times; antioxidant activity, 2.3 times. A high correlation between antioxidant capacity and the content of phenolic compounds (r = 0.90; p <0.001) and tannins (r = 0.94; p <0.001) was revealed. Site conditions have a strong effect both on the accumulation of secondary metabolites in Viburnum opulus L. and on the antioxidant activity of the study sample extracts. The highest concentrations of the phytocomponents under study were associated with heavy precipitation and high average contents of organic matter and macroelements (phosphorus, potassium, and nitrogen). These findings highlight the importance of fulfilling agro-technical requirements for the cultivation of Viburnum opulus L., both to ensure high yields and to obtain products with better biochemical composition.
Evaluation of the stability of the results of studies of beef for lead content using the additive method
Authors: Tretyak L N , Artyukhova S I , Sarbatova N Ju , Aryukhin O V , Zhumanova G T 
Published: March 2021

Abstract
The obtained monitoring results indicate that in the range of less than 0.01 mg of lead per 1 kg of beef prevails (51.9%) over other ranges. The least registered sample results (3.4%) with the range of obtained data - 0.1-1.0 mg / kg. The primary and important task remains to ensure the control of the quantitative content of lead, the introduction and application of fast and reliable methods of their research. The analysis of the obtained data results shows that all the results on the study of beef for the content of lead, obtained by stripping voltammetry and atomic absorption spectrometry methods for assessing the precision and operational control of the error using the additive method, are satisfactory. By implementing the methods of stripping voltammetry and atomic absorption spectrometry, the best precision of research results in the testing laboratory is achieved, both under repeatability conditions and under conditions of intermediate precision.
Effect of amino acid imbalance in the nutrition on the morphology of animal liver
Authors: Omarov M O , Guseynov G O , Gazimagomedova M M , Osmanova S O , Ismayilova F O 
Published: March 2021

Abstract
The article considers the influence of various forms of amino acid imbalance in diets on morphological changes in the liver of laboratory animals. An imbalance of amino acids caused a number of similar changes in the liver of animals. Liver sections revealed that hepatocytes had smoothed borders and areas with signs of granular dystrophy. The sinusoids and inter-protein spaces were overflowed with blood moulds. Small groups of lymphohistiocytic cells were presented in the parenchyma. Violation of the liver microcirculation contributed to the violation of metabolic processes in hepatocytes. Most of the hepatocytes were filled with yellow-orange fat droplets and had atrophied cytoplasm with nuclei shifted to the periphery. There were single cells with glycogen granules as well. The content of nucleic acids in tissues is an important indicator of the intensity of protein synthesis. Thus, in liver sections of groups 1, 2, 3 and 4, the cytoplasm of hepatocytes was less colored than in the control group, which indirectly indicates an increase in the intensity of protein synthesis in liver cells. Hemodynamic disorders in the form of dilated blood vessels and the appearance of stasis in groups 1, 2, 3 and 4 lead to difficulties in the penetration of amino acids to the sites of protein synthesis and, as a result, to a violation of metabolic processes in parenchymal liver cells.
Ecological and biotopic parameters and resilience of arctic bramble (Rubus arcticus L.) in Kirov region, Russia
Authors: Gudovskikh Yu V , Luginina E A , Egoshina T L 
Published: March 2021

Abstract
Ecological-phytocoenotic features of Rubus arcticus L. were estimated near the Southern border of the range, ecological range of the species was also defined. According to the scale of soil humidity fluctuation the conditions’ range of the studied populations is wider than given by D N Tsyganov (1983). According to the complex of ecological scales, R. arcticus is a mesobiont species. Light-shading scale states euryvalent properties, and cumulative spectrum of soil scales shows its stenobiont features. Based on phytoindication data and according to the discomfort index, the most favourable conditions of edapho- and climatope for R. arcticus are formed in graminoid-mixed herbs wet meadow on forest aisle surrounded by birch-aspen bilberry forest and in spruce-birch mixed herbs-graminoid swampy forest. Hemeroby studies showed that the species has low resilience to human impact and can tolerate moderate anthropogenic influence.
Physiological response of the morphological characteristics of mammalian blood to the intake of selenium preparations into the body
Authors: Medvedev I N 
Published: March 2021

Abstract
In the course of numerous studies, the great importance of the trace element selenium for the optimum organism of mammals, including those that are farm animals, has been recognized. Therefore, in modern studies, special attention is paid to the study of the presence of selenium in the diet of animals and parallels are drawn between the level of selenium in feed and the state of the productive and breed characteristics of different animals. Enrichment of feed with selenium in males of different animal species seems promising. The influence of some selenium-containing preparations on the cellular composition of the blood of sire bulls was evaluated. It was found that when a sufficient amount of selenium is introduced into their feed in the form of selenopyran or sodium selenite, physiologically beneficial changes in the morphological parameters of blood develop. It becomes clear that in bulls-producers against the background of the introduction of tested selenium-containing agents in the feed ration, the general resistance of the organism increases, which inevitably leads to an improvement in the quality of sperm production.
Quantitative intestinal ultrasonography for dogs’ parvoviral enteritis
Authors: Tcygansky R A , Nekrasova I I , Mikhailenko V M 
Published: March 2021

Abstract
The article is devoted to the quantitative characteristics of the small intestine wall layers echoicity of dogs with parvoviral enteritis, their ratio and comparison of the results with the index of healthy animals intestinal echoicity. The object of the study was 53 heterogeneous dogs of both sexes with confirmed polymerase chain reaction diagnosed with parvoviral enteritis at the age from 6 weeks to 7 months. The studies were carried out at the Pirogov Veterinary Center of Stavropol and SD&TVC at FSBEI HE “Stavropol State Agrarian University” on the SIUI Apogee 1100 scanner (Shantou Institute of Ultrasonic Instruments Co., Ltd., Guangdong, China) according to the generally accepted method using a multi-frequency linear sensor with a frequency of 7-12 MHz in B-mode. It was found that each layer of the jejunal wall (mucous, submucosal and muscular) of examined dogs was homoechoic and the intestine echoicity index of sick dogs increased for the mucous layer - 2.54 times, submucosal - 1.1, and muscle - in 1.3 times compared with healthy animals’ index. Intestinal echoicity index determination provides objective information about changes in the main ultrasound parameter during ultrasound of alimentary tract structures.
Turpentine oils as a basis for the creation of nanostructured lyosols: physicochemical characteristics and stability
Authors: Chebotarev S N , Fadeeva I S , Chupakhin E G , Zyubin A Yu , Samusev I G , Antipov S S 
Published: March 2021

Abstract
Currently, one of the most common methods for the synthesis of dispersions based on turpentine in aqueous media is the preparation of its solutions in water using multi-stage chemical and/or physical treatment. In this case, the chemical composition is selected in such a way as to ensure the complete dissolution of hydrophobic substances in water. An example of solving such a problem is the use of an aqueous solution of the following composition: gum turpentine 43.0%; oleic acid 25.0%; castor oil 18.0%; caustic soda 3.0%. However, such methods do not allow setting and controlling dimensional parameters in the final product. In addition, in many cases, such a lyosol requires the addition of stabilizers and emulsifiers, which is determined by the purpose of the synthesized lyosol. Here we show the possibility of influencing the size parameter of the resulting particles in lyosol and their resistance to sedimentation in an aqueous solution. This result is achieved by homogenization using ultrasonic dispersion of a low-concentrated aqueous solution of turpentine, bubbling with various gases and subsequent filtration of the resulting emulsion through a membrane with a suitable pore size. This approach makes it possible to obtain at least 95% of nanoparticles from the total dry matter fraction. Compounds based on such lyosols, as a rule, have immunoreactogenic properties and can be used as an adjuvant component that increases the immunogenicity of inactivated vaccines, including for veterinary use.
Using ergotropics to normalize the homeostasis system activity in broiler chickens
Authors: Chernov I S , Semenyutin V V , Chernova E N , Krapivina E V , Kosov A V , Yakovleva I N , Yastrebova O N 
Published: March 2021

Abstract
The article describes the research results on the complex enzyme and antibacterial drugs use in feed for the Hubbard cross broiler chickens. A studies review is presented, which showed that ergotropic preparations complex use when growing broiler chickens is poultry increasing the meat productivity effective method, as per basic zootechnical indicators. As a studying result an ergotropics complex combined use effectiveness in growing poultry, a significant decrease in a highly toxic elements number concentration in the broiler chickens carcasses and organs has been established, which is very important today. The preparation complex influence on the Hubbard cross broiler chickens meat chemical composition, physicochemical and tasting parameters was traced. Our research has proved the ergotropic drugs use effectiveness in broiler chickens, which is one of the increasing poultry meat productivity effective methods and the quality products necessary to provide the population needs with high nutritional and biological products values.
The new sheep ovarian cell culture and the prospect its application in virology
Authors: Galnbek T V , Shulyak A F , Pronina A A , Zhuravleva E A 
Published: March 2021

Abstract
The new sheep ovarian cell culture named as SO was derived and its reproduction has been successful carried out using available growth mediums such as mixture of modified Eagle’s medium, lactalbumin hydrolysate and bovine serum. The monolayer was formed at a seeding concentration of 100-120 thousand cells/ml in 3-5 days. The proliferation index was 2.81-3.24. Fibroblast-like cells predominated at early generations of the culture. During high passages, domination of epithelial-like cells was observed. While passaging, the modal class of 54 chromosomes was retained but the number of chromosome dispersion increased and the number of cells with a diploid set decreased. The cell viability was 82-75% during long-term storage in liquid nitrogen. The monolayer was retained for at least two weeks without changing the medium at 37°C. The culture produced estradiol and progesterone. Cell culture was highly sensitive to bovine herpesvirus 1, parainfluenza-3, nodular dermatitis and pustular stomatitis (Orf) viruses, but had low sensitivity to Orthopneumovirus. It didn’t allow reproduction of pestivirus – causative agent of bovine viral diarrhea – mucosal disease.
Use of antibacterial drugs Ziprovet-Pulmo and Streppen LA in streptococcosis of piglets
Authors: Merzlenko R A , Kovaleva V U , Lavrova O B , Naumova S V , Dolganov Y Y 
Published: March 2021

Abstract
The article presents the research results on the use of antibacterial drugs Ciprovet-pulmo and Streppen LA in Piglet streptococcosis. The experiment was conducted on piglets of a large white breed of the rearing group (aged 41 days) with clinical signs of streptococcosis. The diagnosis of associative streptococcosis was made comprehensively - based on epizootological data, clinical signs of the disease, pathoanatomic changes and laboratory studies of pigs pathological material (liver, spleen, lymph nodes, kidneys, heart, lungs) using bacteriological method (isolation of the streptococcosis pathogen isolate Streptococcus suis). Inclusion in the treatment regimen of piglets of the first group of antibacterial drugs Ciprovet-pulmo and Streppen LA, contributed to the achievement of the maximum therapeutic effect on the 5th day (96 %). In the second group, where Amoxicillin-800 and Amoxisan were used, the maximum therapeutic effect (86 %) occurred on the 6th day.
Effect of organic nutrients on bioleaching of low-grade copper concentrate at different temperatures
Authors: Elkina Yu A , Melamud V S , Bulaev A G 
Published: March 2021

Abstract
The goal of the present work was to study the effect of different organic nutrients (yeast extract and molasses) on the biooxidation of copper concentrate containing chalcopyrite, tennantite, sphalerite, and pyrite in batch experiments at different temperatures (of 40 to 55°C). For the experiments, representatives of microbial groups predominant in biohydrometallurgical processes, bacterium of the genus Sulfobacillus, Acidithiobacillus
caldus, and archaea of the genus Acidiplasma, were used. It was shown that both temperature and addition of organic nutrients in the medium affected the activity of the bioleaching. In the same time, the effect of organic nutrients was significant only at high temperatures (50 and 55°C). In all experiments, biooxidation rate decreased at the highest temperature, 55°C. Since among the strains used in the study, only A. caldus MBC-1 is autotroph, which is able to provide the population with organic nutrients and is not active at 55°C, at this temperature it was not able to maintain activity of other strains. Therefore at 55°C, biooxidation activity strongly depended on the presence of organic nutrients. Despite temperature and presence of organic nutrient affected activity of concentrate biooxidation that was revealed by the differences in pH, Eh values and iron ions concentrations in the medium, copper, zinc and arsenic extraction rates depended on these factors to a lesser extent.
Spatial distribution of moisture deficit on the territory of the south of Western Siberia and Northern Kazakhstan
Authors: Kusainova Aisulu A , Mezentseva Olga V , Tusupbekov Zhanbolat A , Volkovskaya Natalya P 
Published: March 2021

Abstract
The article examines the spatial distribution of the moisture deficit for meteorological stations in the south of Western Siberia and in Northern Kazakhstan in the forest-steppe and steppe natural zones. To calculate the water equivalent of heat and power resources, empirical formulas were used and the water equivalent, moisture deficit and moisture coefficient were determined. In the course of research for specific years of recent decades, the dependence of the moisture deficit on heat and power resources and precipitation has been revealed. With an increase in atmospheric precipitation and a decrease in thermal energy resources of the climate, an increase in the coefficient of moisture in the territory was revealed.
Influence of fractional composition of fuel on engine performance
Authors: Apazhev A K , Shekikhachev Y A , Batyrov V I , Bolotokov A L , Shekikhacheva L Z 
Published: March 2021

Abstract
The fractional composition of the fuel is associated with the operational characteristics of engines, in particular, the temperature regime and start-up, the tendency to form steam locks in the fuel system, and fuel costs. In the case of using fuel with an octane number lower than that required for this engine, knocking occurs. Therefore, the fuel must be knock-resistant, i.e. the ability to burn in the engine at rated speed without explosions. The chemical stability of the fuel is also important - the ability to resist the formation of tar and oxidation during a certain (induction) period at a temperature of 100° C. The physical stability of the fuel is characterized by the absence of light fractions that evaporate during storage and transportation of gasoline. Fuel contamination is caused by the ingress of various mechanical impurities or water into its composition during storage and transportation. The presence of these impurities affects the performance of the fuel system, clogs it, as a result of which it fails, and in winter can lead to its freezing. Thus, the fractional composition of the fuel should be such that a good start of the engine and rapid acceleration of the power plant, low specific fuel consumption, uniform qualitative and quantitative distribution of the combustible mixture over the engine cylinders, minimal wear of pistons and cylinders are ensured, which confirms the relevance of studies aimed at study of the influence of the fractional composition of fuel on the performance of the engine.
Influence of fertilizer system on the dynamics of nitrogen content and balance on newly developed lands
Authors: Shevchenko V A , Soloviev A M , Bondareva G I , Popova N P 
Published: March 2021

Abstract
Under the conditions of the North-West region, the influence of fertilizer systems and precursors on the dynamics of the accumulation of easily hydrolysable nitrogen and the balance of its total content during the development of unproductive sod-podzolic soils of light granulometric composition was studied. For an eight-year research period, the mineral fertilizer system in the cultivation of barley at the planned yield (40 kg/ha) does not compensate for the loss of easily hydrolysable nitrogen. The organo-mineral system (40 t/ha of organic fertilizers or 100 m3/ha of liquid wastewater from livestock complexes) contributes to an increase in the content of easily hydrolysable nitrogen in the arable soil layer by 2.5 … 5.1 and 2.1 … 5.2 mg/kg of soil, compared to control. Stabilization of the balance of the total nitrogen content occurs with an annual application of 6.9 … 7.2 t/ha of solid fraction of manure or 35 … 37 m3/ha of liquid effluents and expanded reproduction of soil fertility is ensured with the incorporation of 9.7 … 11.0 t/ha solid fraction or 49 … 52 m3/ha of liquid waste. The most intensive accumulation of easily hydrolysable nitrogen is observed when using spring rape as a precursor, on the background of which the highest yield of barley was also obtained.
Technical solutions for cleaning drainage water from irrigated areas
Authors: Drovovozova T I , Mariach S A , Panenko N N 
Published: March 2021

Abstract
In recent years, there has been an increasing shortage of fresh water suitable for irrigation, which does not subsequently cause soil salinization. To solve the above problem, it is necessary to reconstruct the collector-drainage system into a circulation system. Using the example of open collectors, the dynamics of the chemical composition during the growing season was studied, which showed its dependence on the intensity of irrigation, the salinity of irrigation water, and the seasonal amount of precipitation. A comprehensive assessment of the suitability of water from open collectors for irrigation in terms of salinity, chloride salinity, sodium and magnesium alkalinization and soda formation has been carried out. The necessity of additional treatment of drainage and waste waters to exclude soil salinization has been established. Technical solutions for the purification of the drainage runoff in the stream and with the help of a mobile treatment plant are proposed. A diagram of a removable filter cassette operating in a honeycomb type for water purification in a stream is presented.