bluuebunny/crossref_metadata_2025_split huggingface dataset:
Inhibiting α1-adrenergic Receptor Signaling Pathway Ameliorates Alzheimer’s Disease Type Pathologies and Behavioral Deficits in an AD Mouse Model
Authors: Yu Zhong-Yuan , Yi Xu , Wang Ye-Ran , Zeng Gui-Hua , Tan Cheng-Rong , Cheng Yuan , Sun Pu-Yang , Liu Zhi-Hao , Wang Yan-Jiang , Liu Yu-Hui 
Published: August 2021

Abstract
Background The role of α1 adrenergic receptors (α1-ARs) signaling pathway in the pathogenesis of Alzheimer’s disease (AD) has rarely been investigated. Clarifying pathophysiological functions of α1-ARs in the AD brain is helpful for better understanding the pathogenesis and screening novel therapeutic target of AD. Methods This study included 2 arms of in vivo investigations: 1) 6-month-old female APPswe/PS1 mice were intravenously treated with AAV-PHP.eB-shRNA (ARs)-GFP or AAV-PHP.eB-GFP for 3 months. 2) 3-month-old female APPswe/PS1 mice were daily treated with 0.5 mg/kg terazosin or equal saline for 6 months. SH-SY5Y cell lines bearing human Amyloid precurssor protein were treated with terazosin or saline for investigating possible mechanisms. Results α1-ARs knockdown mice exhibited improved behavioral performances than control mice. α1-ARs knockdown mice had significantly lower brain amyloid burden, as reflected by soluble Aβ species, compact and total plaques, than control mice. The α1-ARs inhibitor terazosin substantially reduced Aβ deposition, attenuated downstream pathologies including Tau hyperphosphorylation, glial activation, neuronal loss, synaptic dysfunction, and rescued behavioral deficits of APPswe/PS1 mice. In vitro investigation demonstrated that α1-ARs inhibition down-regulated BACE1 expression, and promoted ser9 phosphorylation of GSK-3β, thus reduced Aβ production. Conclusions This study indicates that inhibition of α1-ARs signaling pathway might represent a promising therapeutic strategy for AD.
Tracking the Randomized Rollout of a Veterans Affairs Opioid Risk Management Tool: A Multi-method Implementation Evaluation Using the Consolidated Framework for Implementation Research
Authors: McCarthy Sharon , Chinman Matthew , Rogal Shari , Klima Gloria , Hausmann Leslie , Mor Maria , Shah Mala , Hale Jennifer , Zhang Hongwei , Gordon Adam , Gellad Walid 
Published: August 2021

Abstract
BackgroundThe Veterans Health Administration (VHA) developed the Stratification Tool for Opioid Risk Mitigation (STORM) dashboard to assist VHA clinicians in identifying Veterans at risk for adverse opioid overdose or suicide-related events. In 2018, a national policy was implemented requiring providers at all VHA facilities to complete case reviews of Veterans identified by STORM as very high risk for adverse events. Nationally, facilities were randomized by the type of oversight required when sufficient case reviews were not completed and also by the timing of an increase in the number of required case reviews. As part of a comprehensive assessment of this policy intervention, we aimed to 1) identify barriers and facilitators to implementing case reviews as required in the policy; 2) assess variation across the four arms of the study; and 3) evaluate associations between facility characteristics and implementation barriers and facilitators.MethodsUsing the Consolidated Framework for Implementation Research (CFIR), we developed a semi-structured interview guide to examine barriers to and facilitators of implementing the STORM policy. Staff from 40 purposefully selected facilities who were involved in implementation were invited to participate in telephone interviews. Interview transcripts were coded and then organized into memos, which were numerically rated using the -2 to +2 CFIR rating system for each construct. Descriptive statistics were used to evaluate the mean ratings on each CFIR construct, the associations between ratings and study arm, and three facility characteristics (size, rurality, and level of academic detailing) associated with CFIR ratings. We used the mean CFIR rating for each site to determine which constructs differed between the sites with highest and lowest overall CFIR scores, and these constructs were described in detail. ResultsInterviews with 78 staff at 39 VHA facilities identified a slightly positive (+0.2) overall mean CFIR rating. CFIR ratings were not significantly different between the four study arms, nor associated with facility characteristics. Overall, two important barriers to implementation were CFIR constructs Access to knowledge and information and Evaluating and reflecting. Having time to complete the reviews was a pervasive barrier. Sites with higher overall CFIR scores showed three important facilitators: Leadership engagement, Engaging, and Implementation climate. ConclusionAlthough there was variability in implementation barriers and facilitators across facilities, these were unrelated to study arms and facility characteristics. Leadership, resources, and overall implementation climate were the strongest facilitators of policy implementation.
Early Warning System triggers in Intensive Care Unit after receiving Intrathecal Morphine: A Retrospective Analysis.
Authors: Skelly James Richard Skelly , Jonker Wouter R 
Published: August 2021

Abstract
BackgroundNeuraxial analgesia, including intrathecal morphine(ITM) administration, provides excellent analgesia in the post-abdominal surgery setting. As a corollary, such patients are commonly admitted to HDU/ICU for post-operative monitoring, and as such, bed availability can curtail its use. Reluctance to ward based postoperative care for this cohort is evident. Ward based postoperative care could benefit post-operative analgesic management.MethodsWe reviewed post-operative vital signs constituting the Irish National Early Warning System (INEWS), for general surgical patients admitted to our ICU after ITM administration, as would be undertaken at ward level. Data analysis for a period between January 2015 and August 2018. Inclusion criteria included: abdominal surgery; preoperative ITM and ICU admission post-operatively. Data for the initial 24 hours of ICU admission were obtained, including: AVPU score; respiratory rate(RR); fraction inspired oxygen(FiO2); arterial oxygen saturation(SpO2); heart rate(HR); systolic blood pressure(sBP). Additional measurements include recorded: ITM dose, age, weight, ASA grade, APACHE II Score, visual analogue pain scale scores, analgesic requirements.ResultsThirty patients were included. The ITM dose was 548.3±28.2mcg(mean±SD). Mean maximum constituent INEWS Scores were: 1.7±0.2(RR); 1.5±0.2(SpO2); 1.6±0.3(AVPU); 1.1±0.2(HR); 2.0±0.2(sBP) giving a mean maximum total INEWS score of 4.9±0.3. Four subjects scored ≥7. No patient had INEWS de-escalated for supplemental oxygen and as such the addition of 3 NEWS points for oxygen supplementation would be applied to 96.7% (n=29) of patients. ConclusionsThe current study demonstrates that patients receiving these doses of ITM can exhibit high INEWS scores which would trigger escalation as outlined by the INEWS scoring system if located on a level 1 ward.
Effects of Potassium Management on Physiological Traiats, Enzyme Activity and Cotton Fiber Cellulose Content
Authors: Echer Fábio , Perez Vinicius José Souza , Baltazar Giuliano Oliveira Carnevalli , Silva Gustavo Ricardo Aguiar , Moro Adriana Lima , Gorni Pedro Henrique , Rosolem Ciro Antonio 
Published: August 2021

Abstract
AimsPotassium (K) is prone to be washed out of plant tissues independent of mineralization since it is not strongly bound to organic structures in the plant. Therefore, cover crops can enhance K cycling in cropping systems increasing the nutrient use efficiency by taking it up deep in the soil profile and releasing it on the soil surface. However, it is not clear if this cycling would have an effect on cotton morphophysiology, enzyme activity, and eventually on fiber quality. MethodsPotassium was applied to two cotton cultivars, either to the cover crop ruzigrass (Urochloa ruzisiensis), or to cotton grown after the grass or not, and split between the cover crop and cotton. ResultsCotton leaf area index was increased late in the season by K, with small differences between fertilized treatments, but was highest at full bloom when at least part of the K was applied to cotton. Stomatal conductance was improved, mainly late in the season and when cotton was grown after ruzigrass. Consequently, the enzymatic activity and accumulation of cellulose in the cotton fiber were also increased. ConclusionPotassium fertilization improves cotton physiological parameters such as leaf area index and stomatal conductance, but the effect on enzyme activity depends on the enzyme and on the cotton cultivar. Early cellulose accumulation in the fiber is favored by potassium fertilization and cotton rotation with ruzigrass.
Preparation and Characterization of Hypoxia Sensitive Cationic Liposomal Doxorubicin and Evaluation Its Anti-tumor Activity in Mice Bearing C26 Tumors
Authors: Mashreghi Mohammad , Maleki Mahdi Faal , Askarizadeh Anis , Farshchi Helaleh , Farhoudi Leila , Nasrollahzadeh Mahda Sadat , Hadizadeh Farzin , Jaafari Mahmoud Reza 
Published: August 2021

Abstract
The goal of this study was to prepare cationic nanoliposomal doxorubicin in which PEG molecule attached to the liposome via a hypoxia-sensitive azo linker. The cost-effective hypoxia-sensitive molecule (HSM) was synthesized composing of C18H37 lipophilic tail, azo-linker, and PEG2000 hydrophilic molecule. The NMR and FTIR were employed to characterize the HSM. Then, this compound was post-inserted into the cationic liposome (Cat-lip), and PEG-Azo-Cat-lip was prepared and characterized using DLS. In vitro release and cytotoxicity studies were performed in normoxic and hypoxic conditions. In vivo biodistribution and anti-tumor activities of the formulations were studied on mice bearing C-26 colon carcinoma tumor model and compared with PEGylated liposomal doxorubicin (Caelyx®). Besides, the histological test confirmed the formulation biosafety on healthy mice. The results of NMR and FTIR indicated the synthesis of HSM. The decrease in the zeta-potential of formulation from +18.4 mV for Cat-lip to +6.1 mV along with the increase in the size of the PEG-Azo-Cat-lip indicated the successful post-insertion of HSM. The release study showed that PEGylation results in the more stable PEG-Azo-Cat-lip compared to the Cat-lip. The increased cytotoxicity of the PEG-Azo-Cat-lip in the hypoxic condition also indicated the cleavage of the azo-linker in the hypoxic environment. In vivo biodistribution using animal imaging has shown higher tumor accumulation of the PEG-Azo-Cat-lip than Cat-lip during the 120 h of the study. The results of anti-tumor activities and biosafety of the formulations also showed the higher efficiency of the PEG-Azo-Cat-lip compared with the Cat-lip. The results of this study, indicated the anti-tumor efficacy of this hypoxia-sensitive which merits further investigation.
Fluorescence-guided Surgery of Osteoradionecrosis of the Jaw: A Retrospective Study
Authors: Aljohani Suad , Brunner Teresa Franziska , Chronopoulos Aristeidis , Fliefel Riham , Binmadi Nada , Otto Sven 
Published: August 2021

Abstract
BackgroundOsteoradionecrosis of the jaw (ORNJ) is one of the most severe head and neck complications in patients treated with radiotherapy (RT), and it is one of the diseases difficult to manage. The goal of the treatment is to achieve mucosal healing or suppress ORNJ progression. Currently, surgical removal of the necrotic bone is an effective management approach for advanced stages of ORNJ. This study aimed to identify the outcomes of fluorescence-guided surgery for ORNJ. MethodsNineteen ORNJ lesions in 15 hospitalized patients received fluorescence-guided surgery. Demographics, comorbidities, local preceding event, location, ORNJ stage, and treatment outcomes were retrospectively reviewed with a median follow-up period of 12 months.ResultsThe first 12 lesions (63%) were operated under tetracycline fluorescence, and 7 lesions (37%) were operated under auto-fluorescence. Overall, 4 lesions (21%) achieved complete mucosal healing, 8 lesions (42%) showed partial mucosal healing with bone exposure and no signs or symptoms of inflammation, and 7 lesions (37%) were progressive. The results showed that either healing or ORNJ stabilization was achieved in 63% of the lesions (n = 12). No significant association was observed between healing and the fluorescence technique.ConclusionFluorescence-guided surgery can be beneficial in curing or stabilizing ORNJ. Within the study limitations, autofluorescence-guided surgery seems to be as effective as tetracycline fluorescence-guided surgery for management of ORNJ. Trial registration: not applicable
Potassium-metasomatism in Anthracite: A Cluster of Geochemical Evidences
Authors: Ghosh Santanu , Ojha Anwita , Varma Atul Kumar 
Published: August 2021

Abstract
The present study focuses on the inorganic geochemical features of the bituminous coal samples from the Raniganj and the Jharia Basin, as well as the anthracite samples from the Himalayan fold-thrust belts of Sikkim, India. The SiO2 content (48.05 to 65.09 wt% in the ash yield of the bituminous coal samples and 35.92 to 50.11 wt% in the ash yield of the anthracite samples) and the ratio of Al2O3/TiO2 (6.97 to 17.03 in the bituminous coal samples and 10.34 to 20.07 in the anthracite samples) reveal the intermediate igneous source rock composition of the minerals. The ratio of the K2O/Al2O3 in the ash yield of the bituminous coal samples (0.03 to 0.09) may suggest the presence of kaolinite mixed with montmorillonite, while its range in the ash yield of the anthracite samples (0.16 to 0.27) may imply the presence of illite mixed with kaolinite. The chemical index of alteration values may suggest the moderate to strong chemical weathering of the source rock under sub-humid to humid climatic conditions. The plot of the bituminous coal samples in the A-CN-K diagram depicts the traditional weathering trend of parent rocks, but the anthracite samples plot near the illite field and are a bit offset from the weathering trend. This may imply the plausible influences of the potassium-metasomatism at post coalification stages. The Fourier transform infrared spectra further reveal the hydroxyl stretching intensity of the illite in the anthracite samples substantiating the effect of the epigenetic potassium-metasomatism. The decrease in total kaolinite intensity/compound intensity of quartz and feldspar may provide additional evidence towards this epigenetic event.
Peritoneal Effluent MicroRNA Profile in Encapsulating Peritoneal Sclerosis
Authors: Wu Kun-Lin , Chou Che-Yi , Li An-Lun , Chen Chien-Lung , Tsai Jen-chieh , Wang I-Kuan , Chen Chiung-Tong , Tseng Chin-Chung , Chen Jin-Bor , Chan Yi-Fan , Chang Hui-Yin , Huang Chiu-Ching , Ma Nianhan 
Published: August 2021

Abstract
Background: Encapsulating peritoneal sclerosis (EPS) is a catastrophic complication of chronic peritoneal dialysis (PD). Late diagnosis is associated with high mortality. With the advancement of new diagnostic technologies, such as microRNA (miRNA), we attempted to develop a noninvasive test to assist in the diagnosis of EPS. Methods: MiRNA expression profiles of PD effluents from patients with or without EPS were examined by a high-throughput real-time PCR array to first screen candidate miRNAs. Candidate miRNAs were verified by single real-time PCR. The model for EPS prediction was evaluated by multiple logistic regression. Results: We collected eight-hour PD effluents from 71 non-EPS and 56 EPS patients. The screening set included 28 samples (20 of non-EPS vs. 8 of EPS). After analyzing the ratio values of two miRNA expression levels from the PCR-array of 377 miRNAs, eight candidate miRNAs were selected. The prediction model was conducted using 127 samples (71 of non-EPS vs 56 of EPS) to produce an area under the curve (AUC) value of the miRNA classifier. The ratios of the five miRNAs with the top five ROC values were selected to calculate the combined AUC by logistic regression. The AUC value to detect EPS with the five miRNA ratios was 0.8929 with an accuracy of 78.7%. The accuracy of the EPS diagnosis was further improved to 94.1% after considering clinical characteristics (AUC value 0.9931). Conclusions: A signature-based model of clinical characteristics and miRNA expression in PD effluents can efficiently assist in the diagnosis of EPS, thus preventing the catastrophic prognosis.
Polymethyl Methacrylate Cure Time in Simulated In Vivo Total Knee Arthroplasty Versus In Vitro Conditions
Authors: Funk Daniel , Nguyen Viet , Swank Michael 
Published: August 2021

Abstract
Background: The present means of confirming the cure of intra-operative polymethyl methacrylate (PMMA) cement is to wait for the remainder cement to harden. To our knowledge, there is no available technique to determine the precise moment of cure for in-vivo cement beneath the tibial tray. This study uses a novel means to determine cement curing time in two environments. One environment represents the operating theater, and the other environment attempts to model cement conditions under the tibial tray during surgery.Materials and Methods: We determined the temperature-versus-time plot of cement curing using the following two temperature sensors: one in a simulated implanted tibial tray and another in the remainder cement. We performed 55 tests using dental methyl methacrylate cement mixed in the same ratio as the orthopedic cement. To simulate in vivo conditions, a simulated stainless-steel tibial tray was implanted on a cancellous bone substitute (Sawbones, Vashon Island, WA, USA) using standard cement technique and subsequently placed in a 90°F (32.2 °C) circulating water bath. We positioned a temperature sensor in the cement mantel and positioned a second sensor in a portion of the remaining cement. The temperature from both sensors was measured simultaneously, beginning at 5 mins after mixing and continuing for 20 mins. The first derivative of the temperature provided the precise curing time for each condition. We analyzed the results of 55 repeated experiments with an independent samples t-test. Results: With the described technique, we were able to accurately determine the moment of cure of the cement beneath the simulated tray. There was a mean difference between cure time of 5 mins and 26 s (p-value<0.001) between the two conditions. Conclusions: We validated that our technique was successful in determining the precise time to cure in two different environments.Level of Evidence: This was not a clinical trial and did not involve patients as such the level of evidence was Grade A: Consistent 1 and 2
Effectiveness and Safety of Bedaquiline-containing Regimens for Treatment on Patients With Refractory Rifampicin/multidrug-resistant Tuberculosis: A Retrospective Cohort Study in East China
Authors: Zhang Shao-Jun , Yang Yan , Sun Wen-Wen , Zhang Zhong-Shun , Xiao He-ping , Li Yu-Ping , Zhang Zhe-Min , Fan Lin 
Published: August 2021

Abstract
Objective: To compare the effectiveness and safety of bedaquiline-containing and bedaquiline-free regimens for treatment of patients with refractory rifampicin-resistant/multidrug-resistant tuberculosis (RR/MDR-TB).Methods: Patients with refractory RR/MDR-TB receiving bedaquiline-containing regimens (bedaquiline group, n=102) and bedaquiline-free regimens (non-bedaquiline group, n=100) were included in this retrospective historical control study across East China. The culture conversion, end-of-treatment outcomes, cavity closing rate, and adverse events (AEs) were compared between the two groups. Univariate and multivariate analyses were performed to identify independent predictors of treatment success and culture reversion.Results: The baseline characteristics of the patients were well balanced between the two groups. The culture conversion rates in the bedaquiline group at month 3 (89.2% vs. 66.0%), month 6 (90.2% vs. 72.0%), month 9 (91.2% vs. 66.0%), and month 12 (94.1% vs. 65.0%) were all significantly higher than those in the non-bedaquiline group (all p<0.001). Similar results were observed in the cavity closing rate at month 9 (19.6% vs. 8.0%, p=0.017) and month 12 (39.2% vs. 15.0%, p<0.001). Patients receiving bedaquiline-containing regimens had more treatment success than those receiving bedaquiline-free regimens (p<0.001; cure rate, 69.6% vs. 45.0%; complete the treatment, 22.5% vs. 18.0%; treatment success, 92.2% vs. 63.0%). The use of bedaquiline was identified as an independent predictor of both treatment success (OR=7.356, 95% CI: 2.920–18.530, p<0.001) and culture reversion (OR=0.124, 95% CI: 0.035–0.452, p<0.001). AEs were similarly reported in 26.5% of patients in the bedaquiline group and 19.0% in the non-bedaquiline group (p=0.206).Conclusions: Bedaquiline-containing regimens resulted in better treatment outcomes and similar safety relative to bedaquiline-free regimens for patients with refractory pulmonary RR/MDR-TB.
HOTAIR, HOXC13, and HOXC10 Are Overexpressed in Gastric Cancer
Authors: Habibi Mahvash , Fakhari Reza , Zamani Mina , Galehdari Hamid 
Published: August 2021

Abstract
Gastric cancer is concerned as the second leading cause of tumor-associated death worldwide after lung tumors and is specified as one of the most common malignant cancers. Given the increasing importance of HOX genes in gastric cancer research, this study was aimed at exploring the expression profile of HOTAIR, HOXC13, HOXC10, HOXC13-AS, and HOXC‑AS3 in gastric cancer. To achieve this goal, 30 pairs of tumor and normal margin samples were assessed for these genes expression analyses via qRT-PCR. result we found that, the HOTAIR, HOXC13, and HOXC10 expression was significantly increased in cancer tissue samples in comparison with adjacent normal tissue samples, P<0.01. Moreover, there were significant positive correlations between the expressions of genes studied: HOXC‑AS3 and HOXC10 (r=0.52, P<0.003), HOXC13-AS and HOXC13 (r=0.57, P<0.001), HOTAIR and HOXC‑AS3 (r=0.39, P<0.03), HOTAIR and HOXC13-AS (r=0.36, P<0.05). The HOXC13 and HOXC10 expression exhibited a significant relationship with both distant metastasis and perineural invasion (metastasis: P<0.014, r=0.443 and P<0.0041, r=0.51 perineural invasion: P<0.025, r=0.41 and P<0.0017, r=0.55). The HOXC13-AS displayed a significant relationship with the sex factor so that in females the expression of this gene was higher than males (P<0.030, r=0.4). Our findings suggest that the expression of HOXC genes and HOTAIR lncRNA increased through gastric tumor progression and thus they possibly participate in malignant transformation and gastric carcinoma metastasis.
Process Optimization and Adsorptive Mechanism for Reactive Blue 19 Dye by Magnetic Crosslinked Chitosan/MgO/Fe3O4 Biocomposite
Authors: Jawad Ali H. , S Rangabhashiyam , Abdulhameed Ahmed Saud , Syed-Hassan Syed Shatir A. , ALOthman Zeid A. , Wilson Lee D. 
Published: August 2021

Abstract
A new biocomposite magnetic crosslinked glutaraldehyde-chitosan/MgO/Fe3O4 (CTS-GL/MgO/Fe3O4) adsorbent was prepared and applied for the removal of reactive blue 19 (RB 19) synthetic textile dye. The prepared CTS-GL/MgO/Fe3O4 was subjected to the several instrumental characterizations such as XRD, FTIR, SEM-EDX, pH-potentiometric titration, and pHpzc analyses. The influence of the input adsorption parameters such as A: CTS-GL/MgO/Fe3O4 dosage, B: initial solution pH, C: process temperature, and D: contact time on RB 19 removal efficiency was statistically optimized using Box-Behnken design (BBD). The analysis of variance (ANOVA) indicates the presence of five significant statistical interactions between input adsorption parameters i.e. (AB, AC, AD, BC, and BD). The adsorption kinetic and equilibrium study reveals a good to the pseudo-second-order model, and multilayer adsorption as proven by Freundlich isotherm model, respectively. The maximum adsorption capacity of CTS-GL/MgO/Fe3O4 towards RB19 was found to be 193.2 mg/g at 45 ºC. This work highlights the development of feasible and recoverable magnetic biocompsite adsorbent with desirable adsorption capacity towards textile dyes with good separation ability by using an external magnetic field.