bluuebunny/crossref_metadata_2025_split huggingface dataset:
The diagnostic indicators of gestational diabetes mellitus from second trimester to birth: a systematic review
Authors: Di Filippo Daria , Wanniarachchi Thiyasha , Wei Daniel , Yang Jennifer J. , Mc Sweeney Aoife , Havard Alys , Henry Amanda , Welsh Alec 
Published: October 2021

AbstractBackgroundGestational diabetes mellitus (GDM) is glucose intolerance first recognised during pregnancy. Both modalities and thresholds of the GDM diagnostic test, the Oral Glucose Tolerance Test (OGTT), have varied widely over time and among countries. Additionally, OGTT limitations include inconsistency, poor patient tolerability, and questionable diagnostic reliability. Many biological parameters have been reported to be modified by GDM and could potentially be used as diagnostic indicators. This study aimed to 1) systematically explore biomarkers reported in the literature as differentiating GDM from healthy pregnancies 2) screen those indicators assessed against OGTT to propose OGTT alternatives.Main bodyA systematic review of GDM diagnostic indicators was performed according to PRISMA guidelines (PROSPERO registration CRD42020145499). Inclusion criteria were full-text, comprehensible English-language articles published January 2009-January 2021, where a biomarker (from blood, ultrasound, amniotic fluid, placenta) was compared between GDM and normal glucose tolerance (NGT) women from the second trimester onward to immediately postpartum. GDM diagnostic method had to be clearly specified, and the number of patients per study higher than 30 in total or 15 per group. Results were synthesised by biomarkers.ResultsOf 13,133 studies identified in initial screening, 174 studies (135,801 participants) were included. One hundred and twenty-nine studies described blood analytes, one amniotic fluid analytes, 27 ultrasound features, 17 post-natal features. Among the biomarkers evaluated in exploratory studies, Adiponectin, AFABP, Betatrophin, CRP, Cystatin-C, Delta-Neutrophil Index, GGT, TNF-A were those demonstrating statistically and clinically significant differences in substantial cohorts of patients (> 500). Regarding biomarkers assessed versus OGTT (i.e. potential OGTT alternatives) most promising were Leptin > 48.5 ng/ml, Ficolin3/adiponectin ratio ≥ 1.06, Chemerin/FABP > 0.71, and Ultrasound Gestational Diabetes Score > 4. These all demonstrated sensitivity and specificity > 80% in adequate sample sizes (> / = 100).ConclusionsNumerous biomarkers may differentiate GDM from normoglycaemic pregnancy. Given the limitations of the OGTT and the lack of a gold standard for GDM diagnosis, advanced phase studies are needed to triangulate the most promising biomarkers. Further studies are also recommended to assess the sensitivity and specificity of promising biomarkers not yet assessed against OGTT.Trial registrationPROSPERO registration number CRD42020145499.
Molecular biology of autoinflammatory diseases
Authors: Masumoto Junya , Zhou Wei , Morikawa Shinnosuke , Hosokawa Sho , Taguchi Haruka , Yamamoto Toshihiro , Kurata Mie , Kaneko Naoe 
Published: October 2021

AbstractThe long battle between humans and various physical, chemical, and biological insults that cause cell injury (e.g., products of tissue damage, metabolites, and/or infections) have led to the evolution of various adaptive responses. These responses are triggered by recognition of damage-associated molecular patterns (DAMPs) and/or pathogen-associated molecular patterns (PAMPs), usually by cells of the innate immune system. DAMPs and PAMPs are recognized by pattern recognition receptors (PRRs) expressed by innate immune cells; this recognition triggers inflammation. Autoinflammatory diseases are strongly associated with dysregulation of PRR interactomes, which include inflammasomes, NF-κB-activating signalosomes, type I interferon-inducing signalosomes, and immuno-proteasome; disruptions of regulation of these interactomes leads to inflammasomopathies, relopathies, interferonopathies, and proteasome-associated autoinflammatory syndromes, respectively. In this review, we discuss the currently accepted molecular mechanisms underlying several autoinflammatory diseases.
Insights of Noncanonical Splice-site Variants on RNA Splicing in Patients With Congenital Hypothyroidism
Authors: Albader Najla , Zou Minjing , BinEssa Huda A , Abdi Saba , Al-Enezi Anwar F , Meyer Brian F , Alzahrani Ali S , Shi Yufei 
Published: October 2021

Abstract

Context
Congenital hypothyroidism (CH) is caused by mutations in the genes for thyroid hormone synthesis. In our previous investigation of CH patients, approximately 53% of patients had mutations in either coding exons or canonical splice sites of causative genes. Noncanonical splice-site variants in the intron were detected but their pathogenic significance was not known.

Objective
This work aims to evaluate noncanonical splice-site variants on pre–messenger RNA (pre-mRNA) splicing of CH-causing genes.

Methods
Next-generation sequencing data of 55 CH cases in 47 families were analyzed to identify rare intron variants. The effects of variants on pre-mRNA splicing were investigated by minigene RNA-splicing assay.

Results
Four intron variants were found in 3 patients: solute carrier family 26 member 4 (SLC26A4) c.1544+9C&gt;T and c.1707+94C&gt;T in one patient, and solute carrier family 5 member 5 (SLC5A5) c.970-48G&gt;C and c.1652-97A&gt;C in 2 other patients. The c.1707+94C&gt;T and c.970-48G&gt;C caused exons 15 and 16 skipping, and exon 8 skipping, respectively. The remaining variants had no effect on RNA splicing. Furthermore, we analyzed 28 previously reported noncanonical splice-site variants (4 in TG and 24 in SLC26A4). Among them, 15 variants (~ 54%) resulted in aberrant splicing and 13 variants had no effect on RNA splicing. These data were compared with 3 variant-prediction programs (FATHMM-XF, FATHMM-MKL, and CADD). Among 32 variants, FATHMM-XF, FATHMM-MKL, and CADD correctly predicted 20 (63%), 17 (53%), and 26 (81%) variants, respectively.

Conclusion
Two novel deep intron mutations have been identified in SLC26A4 and SLC5A5, bringing the total number of solved families with disease-causing mutations to approximately 45% in our cohort. Approximately 46% (13/28) of reported noncanonical splice-site mutations do not disrupt pre-mRNA splicing. CADD provides highest prediction accuracy of noncanonical splice-site variants.
CZON-cutter – a CRISPR-Cas9 system for multiplexed organelle imaging in a simple unicellular alga
Authors: Tanaka Naoto , Mogi Yuko , Fujiwara Takayuki , Yabe Kannosuke , Toyama Yukiho , Higashiyama Tetsuya , Yoshida Yamato 
Published: October 2021

ABSTRACT
The unicellular alga Cyanidioschyzon merolae has a simple cellular structure; each cell has one nucleus, one mitochondrion, one chloroplast and one peroxisome. This simplicity offers unique advantages for investigating organellar proliferation and the cell cycle. Here, we describe CZON-cutter, an engineered clustered, regularly interspaced, short palindromic repeats (CRISPR)/CRISPR-associated nuclease 9 (Cas9) system for simultaneous genome editing and organellar visualization. We engineered a C. merolae strain expressing a nuclear-localized Cas9–Venus nuclease for targeted editing of any locus defined by a single-guide RNA (sgRNA). We then successfully edited the algal genome and visualized the mitochondrion and peroxisome in transformants using fluorescent protein reporters with different excitation wavelengths. Fluorescent protein labeling of organelles in living transformants allows us to validate phenotypes associated with organellar proliferation and the cell cycle, even when the edited gene is essential. Combined with the exceptional biological features of C. merolae, CZON-cutter will be instrumental for investigating cellular and organellar division in a high-throughput manner.
This article has an associated First Person interview with the first author of the paper.
Flight training and dietary antioxidants have mixed effects on the oxidative status of multiple tissues in a female migratory songbird
Authors: Frawley Abigail E. , DeMoranville Kristen J. , Carbeck Katherine M. , Trost Lisa , Bryła Amadeusz , Działo Maciej , Sadowska Edyta T. , Bauchinger Ulf , Pierce Barbara J. , McWilliams Scott R. 
Published: October 2021

ABSTRACT
Birds, like other vertebrates, rely on a robust antioxidant system to protect themselves against oxidative imbalance caused by energy-intensive activities such as flying. Such oxidative challenges may be especially acute for females during spring migration, as they must pay the oxidative costs of flight while preparing for reproduction; however, little previous work has examined how the antioxidant system of female spring migrants responds to dietary antioxidants and the oxidative challenges of regular flying. We fed two diets to female European starlings, one supplemented with a dietary antioxidant and one without, and then flew them daily in a windtunnel for 2 weeks during the autumn and spring migration periods. We measured the activity of enzymatic antioxidants (glutathione peroxidase, superoxide dismutase and catalase), non-enzymatic antioxidant capacity (ORAC) and markers of oxidative damage (protein carbonyls and lipid hydroperoxides) in four tissues: pectoralis, leg muscle, liver and heart. Dietary antioxidants affected enzymatic antioxidant activity and lipid damage in the heart, non-enzymatic antioxidant capacity in the pectoralis, and protein damage in leg muscle. In general, birds not fed the antioxidant supplement appeared to incur increased oxidative damage while upregulating non-enzymatic and enzymatic antioxidant activity, though these effects were strongly tissue specific. We also found trends for diet×training interactions for enzymatic antioxidant activity in the heart and leg muscle. Flight training may condition the antioxidant system of females to dynamically respond to oxidative challenges, and females during spring migration may shift antioxidant allocation to reduce oxidative damage.
The effectiveness of EMR implementation regarding reducing documentation errors and waiting time for patients in outpatient clinics: a systematic review
Authors: Albagmi Salem 
Published: October 2021

Background: Electronic medical records (EMRs) refer to the digital copies of paper notes prepared in the physician’s office, outpatient clinics and other departments in health care institutes. EMRs are considered to be significant and preferable to paper records because they allow providers to keep accurate track of patient data and monitoring over time, thus reducing errors, and enhance overall health care quality. The aim of this systematic review was to highlight the significance of EMRs and the effectiveness of implementation regarding reducing documentation errors and waiting time for patients in outpatient clinics. Methods: PubMed, Central, Ovid, Scopus, Science Direct, Elsevier, Cochrane , WHO website and the McMaster University Health Evidence website from 2005-2020 were searched to identify studies that investigated the association between the EMR implementation and documentation error and waiting time for patients. A reviewer screened identified citations and extracted data according to the PRISMA guidelines and data was synthesized in a narrative manner. Results: After full text examination of the articles selected for this literature review, the major themes of relevance that were identified in the context of reducing documentation errors and waiting time for patients in outpatient clinic include: reduction of medical errors because of fewer documentation errors resulting from EMR implementation and reduction of waiting time for patients due to overall improvement of system workflow after use of EMRs. Conclusion: In summary of the reviewed evidence from published material, the implementation of an EMR system in any outpatient setting appears to reduce documentation errors (medication dose errors, issues of prescription errors). It was also seen that in many settings, waiting time for patients in outpatient clinics was reduced with EMR use, while in other settings it was not possible to determine if any significant improvement was seen in this aspect after EMR implementation.
3D printed metasurface for generating a Bessel beam with arbitrary focusing directions
Authors: Qu Mei-Jun , Li Wen-Yu , Zeng Ting , Su Jian-Xun , Song Wei-Li 
Published: October 2021

In this Letter, a metasurface combined with emerging 3D printing technology is proposed. The proposed metasurface regards the simple cube as the unit cell, and the height of the cube is the only variable. A nearly linear transmission phase range covering 360° operating at 20 GHz is obtained when the height is regulated in [2.26 mm, 11.20 mm]. Therefore, the proposed unit cell can be adopted to any metasurface with various functions. Taking the generation of a non-diffractive Bessel beam as an example, two metasurfaces composed of

30

×

30

units with different focusing directions are designed based on non-diffractive theory and the generalized law of refraction. Two prototypes are 3D printed and measured by a near-field scanning system. The measured results validate our design with satisfactory focusing and beam deflection performance. Additionally, the 3D printed metasurface has lower cost and a shorter processing cycle, and avoids metal loss. Therefore, a 3D printed metasurface is an excellent candidate that can be applied in millimeter wave or even higher frequency bands.
Prevalence and associated risk factors for hepatitis B and C viruses among refugee populations living in Mahama, Rwanda: A cross-sectional study
Authors: Kamali Innocent , Barnhart Dale A. , Ndahimana Jean d’Amour , Noor Kassim , Mumporeze Jeanne , Nyirahabihirwe Françoise , Gakuru Jean de la Paix , Musafiri Tumusime , Urusaro Sandra , Makuza Jean Damascene , Serumondo Janvier , Rwamuhinda Dina Denis , Nkundibiza Maurice , Kateera Fredrick , Nshogoza Gallican Rwibasira , Mubiligi Joel M. 
Published: October 2021

Introduction
As part of the integration of refugees into Rwanda’s national hepatitis C elimination agenda, a mass screening campaign for hepatitis B (HBV) and hepatitis C (HCV) was conducted among Burundian refugees living in Mahama Camp, Eastern Rwanda. This cross-sectional survey used data from the screening campaign to report on the epidemiology of viral hepatitis in this setting.

Methods
Rapid diagnostic tests (RDTs) were used to screen for hepatitis B surface antigen (HBsAg) and hepatitis C antibody (anti-HCV) among people of ≥15years old. We calculated seroprevalence for HBsAg and anti-HCV by age and sex and also calculated age-and-sex adjusted risk ratios (ARR) for other possible risk factors.

Results
Of the 26,498 screened refugees, 1,006 (3.8%) and 297 (1.1%) tested positive for HBsAg and Anti-HCV, respectively. HBsAg was more prevalent among men than women and most common among people 25–54 years old. Anti-HCV prevalence increased with age group with no difference between sexes. After adjusting for age and sex, having a household contact with HBsAg was associated with 1.59 times higher risk of having HBsAg (95% CI: 1.27, 1.99) and having a household contact with anti-HCV was associated with 3.66 times higher risk of Anti-HCV (95% CI: 2.26, 5.93). Self-reporting having HBV, HCV, liver disease, or previously screened for HBV and HCV were significantly associated with both HBsAg and anti-HCV, but RDT-confirmed HBsAg and anti-HCV statuses were not associated with each other. Other risk factors for HBsAg included diabetes (ARR = 1.97, 95% CI: 1.08, 3.59) and family history of hepatitis B (ARR = 1.32, 95% CI: 1.11, 1.56) and for anti-HCV included heart disease (ARR = 1.91, 95% CI: 1.30, 2.80) and history of surgery (ARR = 1.70, 95% CI: 1.24, 2.32).

Conclusion
Sero-prevalence and risks factors for hepatitis B and C among Burundian were comparable to that in the Rwandan general population. Contact tracing among household members of identified HBsAg and anti-HCV infected case may be an effective approach to targeted hepatitis screening given the high risk among self-reported cases. Expanded access to voluntary testing may be needed to improve access to hepatitis treatment and care in other refugee settings.
Increased risk of hospitalisation and intensive care admission associated with reported cases of SARS-CoV-2 variants B.1.1.7 and B.1.351 in Norway, December 2020 –May 2021
Authors: Veneti Lamprini , Seppälä Elina , Larsdatter Storm Margrethe , Valcarcel Salamanca Beatriz , Alnes Buanes Eirik , Aasand Nina , Naseer Umaer , Bragstad Karoline , Hungnes Olav , Bøås Håkon , Kvåle Reidar , Golestani Karan , Feruglio Siri , Vold Line , Nygård Karin , Whittaker Robert 
Published: October 2021

IntroductionSince their emergence, SARS-CoV-2 variants of concern (VOC) B.1.1.7 and B.1.351 have spread worldwide. We estimated the risk of hospitalisation and admission to an intensive care unit (ICU) for infections with B.1.1.7 and B.1.351 in Norway, compared to infections with non-VOC.Materials and methodsUsing linked individual-level data from national registries, we conducted a cohort study on laboratory-confirmed cases of SARS-CoV-2 in Norway diagnosed between 28 December 2020 and 2 May 2021. Variants were identified based on whole genome sequencing, partial sequencing by Sanger sequencing or PCR screening for selected targets. The outcome was hospitalisation or ICU admission. We calculated adjusted risk ratios (aRR) with 95% confidence intervals (CIs) using multivariable binomial regression to examine the association between SARS-CoV-2 variants B.1.1.7 and B.1.351 with i) hospital admission and ii) ICU admission compared to non-VOC.ResultsWe included 23,169 cases of B.1.1.7, 548 B.1.351 and 4,584 non-VOC. Overall, 1,017 cases were hospitalised (3.6%) and 206 admitted to ICU (0.7%). B.1.1.7 was associated with a 1.9-fold increased risk of hospitalisation (aRR 95%CI 1.6–2.3) and a 1.8-fold increased risk of ICU admission (aRR 95%CI 1.2–2.8) compared to non-VOC. Among hospitalised cases, no difference was found in the risk of ICU admission between B.1.1.7 and non-VOC. B.1.351 was associated with a 2.4-fold increased risk of hospitalisation (aRR 95%CI 1.7–3.3) and a 2.7-fold increased risk of ICU admission (aRR 95%CI 1.2–6.5) compared to non-VOC.DiscussionOur findings add to the growing evidence of a higher risk of severe disease among persons infected with B.1.1.7 or B.1.351. This highlights the importance of prevention and control measures to reduce transmission of these VOC in society, particularly ongoing vaccination programmes, and preparedness plans for hospital surge capacity.
How does individualised physiotherapy work for people with low back pain? A Bayesian Network analysis using randomised controlled trial data
Authors: Liew Bernard X. W. , Ford Jon J. , Scutari Marco , Hahne Andrew J. 
Published: October 2021

PurposeIndividualised physiotherapy is an effective treatment for low back pain. We sought to determine how this treatment works by using randomised controlled trial data to develop a Bayesian Network model.Methods300 randomised controlled trial participants (153 male, 147 female, mean age 44.1) with low back pain (of duration 6–26 weeks) received either individualised physiotherapy or advice. Variables with potential to explain how individualised physiotherapy works were included in a multivariate Bayesian Network model. Modelling incorporated the intervention period (0–10 weeks after study commencement–“early” changes) and the follow-up period (10–52 weeks after study commencement–“late” changes). Sequences of variables in the Bayesian Network showed the most common direct and indirect recovery pathways followed by participants with low back pain receiving individualised physiotherapy versus advice.ResultsIndividualised physiotherapy directly reduced early disability in people with low back pain. Individualised physiotherapy exerted indirect effects on pain intensity, recovery expectations, sleep, fear, anxiety, and depressionviaits ability to facilitate early improvement in disability. Early improvement in disability, led to an early reduction in depression both directly and via more complex pathways involving fear, recovery expectations, anxiety, and pain intensity. Individualised physiotherapy had its greatest influence on early change variables (during the intervention period).ConclusionIndividualised physiotherapy for low back pain appears to work predominately by facilitating an early reduction in disability, which in turn leads to improvements in other biopsychosocial outcomes. The current study cannot rule out that unmeasured mechanisms (such as tissue healing or reduced inflammation) may mediate the relationship between individualised physiotherapy treatment and improvement in disability. Further data-driven analyses involving a broad range of plausible biopsychosocial variables are recommended to fully understand how treatments work for people with low back pain.Trials registrationACTRN12609000834257.
S7ílhen tl’a Swa7ám̓ (Food of the Ancestors)
Authors: Wyss T’uy’t’tanat-Cease 
Published: October 2021

T’uy’t’tanat-Cease Wyss has dedicated most of her life to Indigenous plant remediation and restoration projects that support wetlands ecosystems, food forests and the wild inhabitants of these places. This is diligent and time based work. It will take several lifetimes to heal our planet, and everything we plant now will help to ensure that our future ancestors will survive.
Through the work of planting and rebuilding these wetlands and food forests we are gifting the future a little of what was greatly taken from the natural world in the past 150 years. Through interdisciplinary documentation of this work, we assure that our future ancestors will continue to be connected to the natural world, by knowing the true names in our Indigenous languages, of these sacred plant relatives.
These photos, along with the Sḵwx̱úmesh Snichem text, are part of the knowledge carrying that must be taken to the next generations to stay informed and connected. This is what reciprocity means to us. This is what Indigenous Food Sovereignty looks like.
ᖃᓪᓗᓈᖅᑕᐃᑦ ᓯᑯᓯᓛᕐᒥᑦ Printed Textiles from Kinngait Studios
Authors: Freeman Na’ama 
Published: October 2021

ᖃᓪᓗᓈᖅᑕᐃᑦ ᓯᑯᓯᓛᕐᒥᑦ Printed Textiles from Kinngait Studios, curated by Roxane Shaughnessy of the Textile Museum of Canada in consultation with independent curator Nakasuq Alariaq, examines the legacy of newly-discovered textile prints from Kinngait Studios and their contribution to art history on both national and global scales. The exhibition shares and preserves a little-known history, drawing connections to contemporary Inuit artistic production and centers Inuit voices in telling this story. At its core, Printed Textiles from Kinngait Studios highlights the powerful way in which visual language can inspire intergenerational connections and jump-start new conversations between artists, community members, and the public at large.
* This review was prepared in partial completion of a masters-level course, and as such, the author did not have the capacity to consult with members of Kinngait community. In future writings, the author hopes to consult and collaborate alongside community members.
Seuchengötter, Heilungsgötter
Authors: Frenschkowski Marco 
Published: October 2021

Abstract
The article discusses the impact of different ideas on epidemic, endemic, and pandemic diseases in antiquity, concentrating on the times of the New Testament and the early church. Different explanations of diseases coexist and to some degree compete: Diseases are sent by God for punishment and admonition, caused by demons or magic, and in ancient medicine caused by strictly natural factors. Contrary to a common cliché, divine punishing is only a minor idea in reflections on disease, and natural causes are generally taken for granted. A demonological interpretation understands disease as something that is fought by Jesus and also by other charismatic healers in ancient Christianity. In this respect, early Christianity can be called a healing movement.