bluuebunny/crossref_metadata_2025_split huggingface dataset:
Loss of the Dβ1 nicotinic acetylcholine receptor subunit disrupts bursicon-driven wing expansion and diminishes adult viability in Drosophila melanogaster
Authors: Christesen Danielle , Yang Ying Ting , Chen Wei , Batterham Philip , Perry Trent 
Published: July 2021

Abstract
Cholinergic signaling dominates the insect central nervous system, contributing to numerous fundamental pathways and behavioral circuits. However, we are only just beginning to uncover the diverse roles different cholinergic receptors may play. Historically, insect nicotinic acetylcholine receptors have received attention due to several subunits being key insecticide targets. More recently, there has been a focus on teasing apart the roles of these receptors, and their constituent subunits, in native signaling pathways. In this study, we use CRISPR-Cas9 genome editing to generate germline and somatic deletions of the Dβ1 nicotinic acetylcholine receptor subunit and investigate the consequences of loss of function in Drosophila melanogaster. Severe impacts on movement, male courtship, longevity, and wing expansion were found. Loss of Dβ1 was also associated with a reduction in transcript levels for the wing expansion hormone bursicon. Neuron-specific somatic deletion of Dβ1 in bursicon-producing neurons (CCAP-GAL4) was sufficient to disrupt wing expansion. Furthermore, CCAP-GAL4-specific expression of Dβ1 in a germline deletion background was sufficient to rescue the wing phenotype, pinpointing CCAP neurons as the neuronal subset requiring Dβ1 for the wing expansion pathway. Dβ1 is a known target of multiple commercially important insecticides, and the fitness costs exposed here explain why field-isolated target-site resistance has only been reported for amino acid replacements and not loss of function. This work reveals the importance of Dβ1-containing nicotinic acetylcholine receptors in CCAP neurons for robust bursicon-driven wing expansion.
Next Steps in Integrative Biology: Mapping Interactive Processes Across Levels of Biological Organization
Authors: Cavigelli Sonia , Leips Jeff , Xiang Qiu-Yun (Jenny) , Lemke Dawn , Konow Nicolai 
Published: July 2021

Synopsis
Emergent biological processes result from complex interactions within and across levels of biological organization, ranging from molecular to environmental dynamics. Powerful theories, database tools, and modeling methods have been designed to characterize network connections within levels, such as those among genes, proteins, biochemicals, cells, organisms, and species. Here, we propose that developing integrative models of organismal function in complex environments can be facilitated by taking advantage of these methods to identify key nodes of communication across levels of organization. Mapping key drivers or connections among levels of organization will provide data and leverage to model potential rule-sets by which organisms respond and adjust to perturbations at any level of biological organization.
Social determinants and facility type impact adherence to best practices in operable IIIAN2 lung cancer
Authors: Muslim Zaid , Stroever Stephanie , Baig Mirza Zain , Weber Joanna F , Connery Cliff P , Bhora Faiz Y 
Published: July 2021

Abstract OBJECTIVESWe aimed to identify patient- and facility-specific predictors of collective adherence to 4 recommended best treatment practices in operable IIIAN2 non-small-cell lung cancer (NSCLC) and test the hypothesis that collective adherence is associated with superior survival.METHODSWe queried the National Cancer Database for clinical stage IIIAN2 NSCLC patients undergoing surgery during 2010–2015. The following best practices were examined: performance of an anatomic resection, performance of an R0 resection, examination of regional lymph nodes and administration of induction therapy. Multivariable regression models were fitted to identify independent predictors of guideline-concordance.RESULTSWe identified 7371 patients undergoing surgical resection for IIIAN2 lung cancer, of whom 90.8% underwent an anatomic resection, 88.2% received an R0 resection, 92.5% underwent a regional lymph node examination, 41.6% received induction therapy and 33.7% received all 4 best practices. Higher income, private insurance and treatment at an academic facility were independently associated with adherence to all 4 best practices (P &lt; 0.01). A lower level of education and residence in a rural county were associated with a lack of adherence (P &lt; 0.05). Adherence to all 4 practices correlated with improved survival (P &lt; 0.01).CONCLUSIONSNational adherence to best treatment practices in operable IIIAN2 lung cancer was variable as evidenced by the majority of patients not receiving recommended induction therapy. Socioeconomic factors and facility type are important determinants of guideline-concordance. Future efforts to improve outcomes should take this into account since guideline concordance, in the form of collective adherence to all 4 best practices, was associated with improved survival.
Stirring the Justice Imagination: Countering the Invisibilization and Erasure of Syrian Victims’ Justice Narratives
Authors: Herremans Brigitte , Destrooper Tine 
Published: July 2021

Abstract
With most avenues to pursue justice for Syrian victims of international crimes blocked, Syrian and international justice actors within civil society and formal institutions are active in exploring ways to seek some form of justice and accountability. In doing so, many of them use the language of transitional justice as the most promising paradigm to keep justice on the international agenda and to resist the prevailing defeatism about the possibility to advance justice in the absence of a transition, as well as to remedy the marginalization of victims’ experiences and narratives. Many of these actors are not only interested in criminal accountability. They also seek to stretch the boundaries of what is imaginable in terms of justice, beyond existing mechanisms and even beyond the judicial realm. They are striving to open up the justice imagination. In a maximalist sense, they are foraging for more ambitious justice narratives that can accommodate the victims’ lived experiences. In a minimalist sense, they are resisting the erasure or invisibilization of the experiences of millions of Syrians affected by hackneyed justice narratives. This article refers to the work of these justice actors to expose and conceptualize some of the shortcomings of mainstream transitional justice discourses.
Shanks and Anderson-type acceleration techniques for systems of nonlinear equations
Authors: Brezinski Claude , Cipolla Stefano , Redivo-Zaglia Michela , Saad Yousef 
Published: July 2021

Abstract
This paper examines a number of extrapolation and acceleration methods and introduces a few modifications of the standard Shanks transformation that deal with general sequences. One of the goals of the paper is to lay out a general framework that encompasses most of the known acceleration strategies. The paper also considers the Anderson Acceleration (AA) method under a new light and exploits a connection with quasi-Newton methods in order to establish local linear convergence results of a stabilized version of the AA method. The methods are tested on a number of problems, including a few that arise from nonlinear partial differential equations.
The Intractability of Islamist Insurgencies: Islamist Rebels and the Recurrence of Civil War
Authors: Nilsson Desirée , Svensson Isak 
Published: July 2021

AbstractThere is a large research field focusing on the recurrence of civil wars, yet this literature has omitted to seriously consider religious dimensions and ideational features of armed conflicts. To address this gap, we provide the first global study exploring whether, and why, Islamist civil wars—armed conflicts fought over self-proclaimed Islamist aspirations—are more or less likely to recur compared to other conflicts. We argue that civil wars fought over Islamist claims are more likely to relapse because the ideational features of these conflicts increase the uncertainty regarding the capabilities of the warring actors in terms of the extent and nature of transnational support that may be forthcoming, for rebels as well as the government. In line with our argument, we find that Islamist civil wars are significantly less likely to be terminated and more likely to recur once ended. Thus, our results demonstrate that Islamist civil wars represent a particular challenge with regard to the goal of achieving durable peace.
Improved Light Traps for Early Detection of Insect Pests of Phytosanitary Concern in Shipping Containers
Authors: Marchioro Matteo , Faccoli Massimo 
Published: July 2021

Abstract
The number of introductions of alien insect has been increasing in the last decades, primarily transported in shipping containers. The attraction of light of different wavelengths (white, infrared, ultraviolet, and red) applied on sticky traps was tested for the development of new traps for hitchhiker insects. The addition of entomological glue and insecticide on the trap was also tested. Tests were conducted on Cadra cautella Walker (Lepidoptera: Pyralidae), Drosophila melanogaster Meigen (Diptera: Drosophilidae), Sitophilus zeamais Motschulsky (Coleoptera: Curculionidae), and Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae) and released inside a shipping container. In the first test, one light color at a time was tested setting eight traps in the container, one for each possible combination of the variables: light on or off, glue added or not, and insecticide sprayed or not. In the second, five traps were used, all of them coated with the entomological glue: one for each light color and one with light off as control. In all the single color tests (except for infrared), light-on traps captured more, except for T. castaneum that was not attracted to white. In the multi-color test, C. cautella showed no preference among white, ultraviolet, or red; Drosophila melanogaster preferred ultraviolet and white over red; and beetles had a much greater attraction to red. Lastly, the stronger entomological glue improved catches of beetles, whereas insecticides did not. In conclusion, results suggest a possible application of sticky light traps against hitchhiker insects and further studies should verify if the simultaneous use of different light colors can improve the trap performance and does not act as a repellent.
The Crime of Unconstitutional Change of Government
Authors: García-Casas María 
Published: July 2021

Abstract
The entry into force of the Protocol on Amendments to the Protocol on the Statute of the African Court of Justice and Human Rights (hereinafter the Malabo Protocol) will imply the activation of the International Criminal Law Section of the African Court of Justice and Human and Peoples’ Rights, along with the new crimes there included — among them, the crime of unconstitutional change of government. While the African Union (AU)’s concern over these changes of government is understandable, the criminalization of them raises several doubts. First, the lack of precision in the wording makes it difficult for both judges and individuals to know what is prohibited and what is not. This, along with the AU’s approach to popular uprisings, entails a serious risk for the Criminal Section of the Court to be used in accordance with political considerations of the regional organization. Finally, the combination of the crime with the immunity clause, also contained in the Protocol, greatly impedes the achievement of the criminalization’s goal.
Lateral roots but not root hairs contribute to high uptake of manganese and cadmium in rice
Authors: Yu En , Yamaji Naoki , Mao Chuanzao , Wang Hua , Ma Jian Feng 
Published: July 2021

Abstract
Rice (Oryza sativa L.) can accumulate high manganese (Mn) in the shoots through uptake by the roots, which consist of crown roots, lateral roots and root hairs. We investigated the role of lateral roots and root hairs in Mn and cadmium (Cd) uptake by using two indica rice mutants defective in formation of lateral roots (osiaa11) and root hairs (osrhl1). The uptake of Mn and Cd in osiaa11 was significantly lower than that in wild type ‘Kasalath’, but there was no difference between wild type and osrhl1. Furthermore, a kinetic study showed that Mn uptake in osiaa11 was much lower than that in wild type and osrhl1 across a wide range of Mn concentrations. The role of lateral roots in Mn and Cd uptake was further confirmed in a japonica rice mutant defective in lateral root formation. We found that expression of Mn transporter gene Natural Resistance-Associated Macrophage Protein 5 (OsNRAMP5), but not of Metal Tolerance Protein 9 (OsMTP9), was lower in osiaa11 than in wild type; however, there were no differences between osrhl1 and the wild type. Immunostaining showed that OsNRAMP5 and OsMTP9 were localized in the exodermis and endodermis of crown roots and lateral roots, but not in the root hairs. Taken together, our results indicate that lateral roots, but not root hairs, play an important role in high Mn and Cd uptake in rice.
From A to AB: A Caucasian Mother with High Anti-B Titer Causing Hemolytic Disease of the Newborn
Authors: Schooley Nathanael , Chabra Shilpi , Stolla Moritz 
Published: July 2021

Abstract
We report on a term infant with clinically significant hemolysis and hyperbilirubinemia. Testing revealed ABO incompatibility between maternal type A and infant type AB. The maternal alloantibody screen was negative. The infant’s direct antiglobulin test was positive, and anti-B IgG was eluted off the infant’s red blood cells (RBCs). Testing of the mother’s plasma revealed a high anti-B titer. The infant was successfully treated with phototherapy and intravenous immunoglobulin. The bilirubin and hematocrit stabilized, and the infant was discharged home. This case was unusual because of its severity and unusual ABO constellation. Furthermore, this report is an exemplary educational case study on how effective collaboration between the clinical team and the blood bank laboratory is critical in reaching the correct diagnosis. In summary, the differential diagnosis of more unusual and atypical ABO-incompatible constellations must be considered when an infant presents with unexplained hemolysis.
How many explosions does one need? Quantifying supernovae in globular clusters from iron abundance spreads
Authors: Wirth Henriette , Jerabkova Tereza , Yan Zhiqiang , Kroupa Pavel , Haas Jaroslav , Šubr Ladislav 
Published: July 2021

ABSTRACT
Many globular clusters (GCs) are known to host multiple populations distinguishable by their light-element content. Less common are GCs displaying iron abundance spreads that are seen as evidence for enrichment through core collapse supernovae (SNe). We present a simple analytical method to estimate the number of SNe required to have occurred in a GC from its metallicity and iron abundance spread. We then use this result to estimate how long star formation (SF) lasted to build the GC. We apply our method to up-to-date measurements and find that, assuming the correctness of these measurements, multiple SNe (up to 105) are required in most GCs with iron abundance spreads. The number of SNe events that contributed to the enrichment of the GCs studied here is typically a factor of 10 less than the expected number of SNe in a canonical initial mass function (IMF). This indicates that gas expulsion from the forming GC occurred after the first 10 per cent of SNe exploded. We compute that for the GCs typically SF ends after only a few Myr (extending up to ${\approx}30 \, \rm Myr$ in a few cases). We also discuss possible improvements of this method and especially its sensitivity to the error of iron abundance measurements of individual stars of a GC. The method presented here can quickly give an estimate for the number of SNe required to explain the iron abundance spread in a GC without the requirement of any hydrodynamical simulations.
Theoretical properties of the autocovariance of wavefront curvature induced by atmospheric turbulence and their potential for Cn2 profiling
Authors: Avila Remy 
Published: July 2021

ABSTRACT
Remote measurements of optical turbulence profiles $C_\mathrm{ \mathit{ n}}^2(h)$ in the Earth atmosphere are nowadays performed from the spatial autocovariance of either scintillation or the wavefront slope. Here, I theoretically study the properties of the spatio-angular autocovariance of the wavefront curvature induced by optical turbulence, assuming the von Kármán model for the complex amplitude fluctuations. The width of the curvature autocovariance function is approximately 10 times smaller than that of the scintillation and 1000 times smaller than that of the wavefront slope. Thus, a significant enhancement of the attainable altitude resolution of turbulence profiles is expected from the spatio-angular autocovariance of the curvature. The curvature autocovariance amplitude is proportional to $C_\mathrm{ \mathit{ n}}^2(h)$ and also depends on the turbulence altitude h and on the turbulence inner scale l0. A simple procedure disentangles those dependencies and leads to $C_\mathrm{ \mathit{ n}}^2(h)$ and also to the inner scale profile l0(h).
Systematic identification of novel cancer genes through analysis of deep shRNA perturbation screens
Authors: Montazeri Hesam , Coto-Llerena Mairene , Bianco Gaia , Zangene Ehsan , Taha-Mehlitz Stephanie , Paradiso Viola , Srivatsa Sumana , de Weck Antoine , Roma Guglielmo , Lanzafame Manuela , Bolli Martin , Beerenwinkel Niko , von Flüe Markus , Terracciano Luigi M , Piscuoglio Salvatore , Ng Charlotte K Y 
Published: July 2021

Abstract
Systematic perturbation screens provide comprehensive resources for the elucidation of cancer driver genes. The perturbation of many genes in relatively few cell lines in such functional screens necessitates the development of specialized computational tools with sufficient statistical power. Here we developed APSiC (Analysis of Perturbation Screens for identifying novel Cancer genes) to identify genetic drivers and effectors in perturbation screens even with few samples. Applying APSiC to the shRNA screen Project DRIVE, APSiC identified well-known and novel putative mutational and amplified cancer genes across all cancer types and in specific cancer types. Additionally, APSiC discovered tumor-promoting and tumor-suppressive effectors, respectively, for individual cancer types, including genes involved in cell cycle control, Wnt/β-catenin and hippo signalling pathways. We functionally demonstrated that LRRC4B, a putative novel tumor-suppressive effector, suppresses proliferation by delaying cell cycle and modulates apoptosis in breast cancer. We demonstrate APSiC is a robust statistical framework for discovery of novel cancer genes through analysis of large-scale perturbation screens. The analysis of DRIVE using APSiC is provided as a web portal and represents a valuable resource for the discovery of novel cancer genes.
Anatomy and White Matter Connections of the Superior Parietal Lobule
Authors: Lin Yueh-Hsin , Dadario Nicholas B , Hormovas Jorge , Young Isabella M , Briggs Robert G , MacKenzie Alana E , Palejwala Ali H , Fonseka R Dineth , Kim Sihyong J , Tanglay Onur , Fletcher Luke R , Abraham Carol J , Conner Andrew K , O’Donoghue Daniel L , Sughrue Michael E 
Published: July 2021

Abstract

BACKGROUND
The superior parietal lobule (SPL) is involved in somatosensory and visuospatial integration with additional roles in attention, written language, and working memory. A detailed understanding of the exact location and nature of associated white matter tracts could improve surgical decisions and subsequent postoperative morbidity related to surgery in and around this gyrus.

OBJECTIVE
To characterize the fiber tracts of the SPL based on relationships to other well-known neuroanatomic structures through diffusion spectrum imaging (DSI)-based fiber tracking validated by gross anatomical dissection as ground truth.

METHODS
Neuroimaging data of 10 healthy, adult control subjects was obtained from a publicly accessible database published in Human Connectome Project for subsequent tractographic analyses. White matter tracts were mapped between both cerebral hemispheres, and a lateralization index was calculated based on resultant tract volumes. Post-mortem dissections of 10 cadavers identified the location of major tracts and validated our tractography results based on qualitative visual agreement.

RESULTS
We identified 9 major connections of the SPL: U-fiber, superior longitudinal fasciculus, inferior longitudinal fasciculus, inferior fronto-occipital fasciculus, middle longitudinal fasciculus, extreme capsule, vertical occipital fasciculus, cingulum, and corpus callosum. There was no significant fiber lateralization detected.

CONCLUSION
The SPL is an important region implicated in a variety of tasks involving visuomotor and visuospatial integration. Improved understanding of the fiber bundle anatomy elucidated in this study can provide invaluable information for surgical treatment decisions related to this region.
Cerebral Bypass for Aneurysms in the Era of Flow Diversion: Single-Surgeon Case Series
Authors: Nouri Mohsen , Schneider Julia R , Shah Kevin , White Timothy G , Katz Jeffrey M , Dehdashti Amir R 
Published: July 2021

Abstract

BACKGROUND
With recent advances in endovascular treatments of brain aneurysms such as flow diverters, the role of cerebral revascularization needs to be re-evaluated.

OBJECTIVE
To evaluate the contemporary indications and outcomes of cerebral revascularization for brain aneurysms.

METHODS
A retrospective evaluation of a prospectively maintained database was performed to review clinical and imaging data of all the patients who underwent cerebral revascularization for brain aneurysms over the past 10 yr.

RESULTS
Among 174 cerebral revascularizations, 40 (in 36 patients) were done for the treatment of aneurysms. In total, 9 patients underwent combined endovascular treatment and surgical revascularization. Immediate aneurysm occlusion was achieved in 30 patients (83.3%). Immediate postoperative bypass patency was confirmed in 33 patients (92%). Postoperative neurological deficit was observed in 4 patients (11.1%). There were 2 mortalities in the postoperative period. Aneurysm total occlusion rate was 91% at 1 yr. Thirty patients had 1 yr clinical and radiological follow-up. Clinical evaluations showed modified Rankin Scale 2 or less in 25 patients at 1 yr. Bypass patency was confirmed in 27 (90%). Patients with fair/poor outcome were all in the subarachnoid hemorrhage group. Twenty-one patients had follow-up studies for 3 yr or beyond with no evidence of stroke or aneurysm recurrence.

CONCLUSION
Our results support that cerebral revascularization can be regarded as a viable and durable treatment option for these challenging aneurysms with acceptable morbidity. Cerebral bypass should be offered in selected cases where standard endovascular or surgical treatment is not efficacious or curative.