bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cyclooxygenase (COX)-2 modulates Toxoplasma gondii infection, immune response and lipid droplets formation in human trophoblast cells and villous explants
Authors: de Souza Guilherme , Silva Rafaela José , Milián Iliana Claudia Balga , Rosini Alessandra Monteiro , de Araújo Thádia Evelyn , Teixeira Samuel Cota , Oliveira Mário Cézar , Franco Priscila Silva , da Silva Claudio Vieira , Mineo José Roberto , Silva Neide Maria , Ferro Eloisa Amália Vieira , Barbosa Bellisa Freitas 
Published: June 2021

AbstractCongenital toxoplasmosis is represented by the transplacental passage ofToxoplasma gondiifrom the mother to the fetus. Our studies demonstrated thatT. gondiideveloped mechanisms to evade of the host immune response, such as cyclooxygenase (COX)-2 and prostaglandin E2(PGE2) induction, and these mediators can be produced/stored in lipid droplets (LDs). The aim of this study was to evaluate the role of COX-2 and LDs duringT. gondiiinfection in human trophoblast cells and villous explants. Our data demonstrated that COX-2 inhibitors decreasedT. gondiireplication in trophoblast cells and villous. In BeWo cells, the COX-2 inhibitors induced an increase of pro-inflammatory cytokines (IL-6 and MIF), and a decrease in anti-inflammatory cytokines (IL-4 and IL-10). In HTR-8/SVneo cells, the COX-2 inhibitors induced an increase of IL-6 and nitrite and decreased IL-4 and TGF-β1. In villous explants, the COX-2 inhibitors increased MIF and decreased TNF-α and IL-10. Furthermore,T. gondiiinduced an increase in LDs in BeWo and HTR-8/SVneo, but COX-2 inhibitors reduced LDs in both cells type. We highlighted that COX-2 is a key factor toT. gondiiproliferation in human trophoblast cells, since its inhibition induced a pro-inflammatory response capable of controlling parasitism and leading to a decrease in the availability of LDs, which are essentials for parasite growth.
Structure sampling for computational estimation of localized DNA interaction rates
Authors: Kumar Sarika , Weisburd Julian M. , Lakin Matthew R. 
Published: June 2021

AbstractMolecular circuits implemented using molecular components tethered to a DNA tile nanostructure have certain advantages over solution-phase circuits. Tethering components in close proximity increases the speed of reactions by reducing diffusion and improves scalability by enabling reuse of identical DNA sequences at different locations in the circuit. These systems show great potential for practical applications including delivery of diagnostic and therapeutic molecular circuits to cells. When modeling such systems, molecular geometry plays an important role in determining whether the two species interact and at what rate. In this paper, we present an automated method for estimating reaction rates in tethered molecular circuits that takes the geometry of the tethered species into account. We probabilistically generate samples of structure distributions based on simple biophysical models and use these to estimate important parameters for kinetic models. This work provides a basis for subsequent enhanced modeling and design tools for localized molecular circuits.
Effect of permeability and fractures on oil mobilization of unconventional resources during CO2 EOR using nuclear magnetic resonance
Authors: Sun Huanquan , Wang Haitao , Lun Zengmin 
Published: June 2021

AbstractCO2 EOR (enhanced oil recovery) will be one of main technologies of enhanced unconventional resources recovery. Understanding effect of permeability and fractures on the oil mobilization of unconventional resources, i.e. tight oil, is crucial during CO2 EOR process. Exposure experiments based on nuclear magnetic resonance (NMR) were used to study the interaction between CO2 and tight oil reservoirs in Chang 8 layer of Ordos Basin at 40 °C and 12 MPa. Effect of permeability and fractures on oil mobilization of exposure experiments were investigated for the different exposure time. The oil was mobilized from matrix to the surface of matrix and the oil recovery increased as the exposure time increased. The final oil recovery increased as the core permeability increased in these exposure experiments. Exposure area increased to 1.75 times by fractures resulting in that oil was mobilized faster in the initial stage of exposure experiment and the final oil recovery increased to 1.19 times from 28.8 to 34.2%. This study shows the quantitative results of effect of permeability and fractures on oil mobilization of unconventional resources during CO2 EOR, which will support CO2 EOR design in Chang 8 layer of Ordos Basin.
Earlier onset of proteinuria or hypertension is a predictor of progression from gestational hypertension or gestational proteinuria to preeclampsia
Authors: Morikawa Mamoru , Mayama Michinori , Noshiro Kiwamu , Saito Yoshihiro , Nakagawa-Akabane Kinuko , Umazume Takeshi , Chiba Kentaro , Kawaguchi Satoshi , Watari Hidemichi 
Published: June 2021

AbstractAlthough gestational hypertension (GH) is a well-known disorder, gestational proteinuria (GP) has been far less emphasized. According to international criteria, hypertensive disorders of pregnancy include GH but not GP. Previous studies have not revealed the predictors of progression from GP to preeclampsia or those of progression from GH to preeclampsia. We aimed to determine both sets of predictors. A retrospective cohort study was conducted with singleton pregnant women who delivered at 22 gestational weeks or later. Preeclampsia was divided into three types: new onset of hypertension/proteinuria at 20 gestational weeks or later and additional new onset of other symptoms at < 7 days or at ≥ 7 days later. Of 94 women with preeclampsia, 20 exhibited proteinuria before preeclampsia, 14 experienced hypertension before preeclampsia, and 60 exhibited simultaneous new onset of both hypertension and proteinuria before preeclampsia; the outcomes of all types were similar. Of 34 women with presumptive GP, 58.8% developed preeclampsia; this proportion was significantly higher than that of 89 women with presumptive GH who developed preeclampsia (15.7%). According to multivariate logistic regression models, earlier onset of hypertension/proteinuria (before or at 34.7/33.9 gestational weeks) was a predicator for progression from presumptive GH/GP to preeclampsia (odds ratios: 1.21/1.21, P value: 0.0044/0.0477, respectively).
Optical manipulation of a dielectric particle along polygonal closed-loop geometries within a single water droplet
Authors: Park Junbum , Hong Seongjin , Lee Yong Soo , Lee Hyeonwoo , Kim Seokjin , Dholakia Kishan , Oh Kyunghwan 
Published: June 2021

AbstractWe report a new method to optically manipulate a single dielectric particle along closed-loop polygonal trajectories by crossing a suite of all-fiber Bessel-like beams within a single water droplet. Exploiting optical radiation pressure, this method demonstrates the circulation of a single polystyrene bead in both a triangular and a rectangle geometry enabling the trapped particle to undergo multiple circulations successfully. The crossing of the Bessel-like beams creates polygonal corners where the trapped particles successfully make abrupt turns with acute angles, which is a novel capability in microfluidics. This offers an optofluidic paradigm for particle transport overcoming turbulences in conventional microfluidic chips.
Application of uniform design to evaluate the different conditions on the growth of algae Prymnesium parvum
Authors: Yin Juan , Sun Xuyang , Zhao Ruizhi , Qiu Xiaocong , Eeswaran Rasu 
Published: June 2021

AbstractPrymnesium parvum is an environmentally harmful algae and well known for its toxic effects to the fish culture. However, there is a dearth of studies on the growth behavior of P. parvum and information on how the availability of nutrients and environmental factors affect their growth rate. To address this knowledge gap, we used a uniform design approach to quantify the effects of major nutrients (N, P, Si and Fe) and environmental factors (water temperature, pH and salinity) on the biomass density of P. parvum. We also generated the growth model for P. parvum as affected by each of these nutrients and environmental factors to estimate optimum conditions of growth. Results showed that P. parvum can reach its maximum growth rate of 0.789, when the water temperature, pH and salinity is 18.11 °C, 8.39, and 1.23‰, respectively. Moreover, maximum growth rate (0.895–0.896) of P. parvum reached when the concentration of nitrogen, phosphorous, silicon and iron reach 3.41, 1.05, 0.69 and 0.53 mg/l, respectively. The order of the effects of the environmental factors impacting the biomass density of P. parvum was pH > salinity > water temperature, while the order of the effects of nutrients impacting the biomass density of P. parvum was nitrogen > phosphorous > iron > silicon. These findings may assist to implement control measures of the population of P. parvum where this harmful alga threatens aquaculture industry in the waterbodies such as Ningxia region in China.
A 2-year prospective multicenter study of ultrasound cyclo plasty for glaucoma
Authors: Giannaccare Giuseppe , Pellegrini Marco , Bernabei Federico , Urbini Lara , Bergamini Fulvio , Ferro Desideri Lorenzo , Bagnis Alessandro , Biagini Francesco , Cassottana Paola , Del Noce Chiara , Carnevali Adriano , Scorcia Vincenzo , Traverso Carlo E. , Vagge Aldo 
Published: June 2021

AbstractUltrasound cyclo plasty (UCP) is a recently developed surgical technique for glaucoma allowing a selective and controlled coagulation of the ciliary body. We herein investigated the long-term efficacy and safety of UCP for the treatment of glaucoma. This prospective study included patients with primary and secondary glaucoma. All surgeries were performed using the EyeOP1 device (Eye Tech Care, Rillieux-la-Pape, France). Sixty-six patients were included, and 60 completed regularly the 2-year follow-up. Preoperative IOP was 28.5 ± 9.6 mmHg and significantly decreased to 17.0 ± 5.4 at 2 years (p < 0.001). The daily number of both hypotensive eye drops and acetazolamide tablets decreased significantly (respectively, from 2.6 ± 1.1 to 1.7 ± 1.2 and from 0.7 ± 0.8 to 0.2 ± 0.5; both p < 0.001). At 2 years, 68.1% of patients met the definition of qualified success (IOP < 21 mmHg regardless of glaucoma medications) and 10.3% of patients met the definition of complete success (IOP < 21 mmHg without glaucoma medications). No major intra- or postoperative complications occurred; however, 15 eyes required additional glaucoma surgery. These results suggest that UCP is an effective and safe procedure to reduce IOP in glaucoma patients through a 2-year follow-up period.
A millennium of trophic stability in Atlantic cod (Gadus morhua): transition to a lower and converging trophic niche in modern times
Authors: Ólafsdóttir Guðbjörg Ásta , Edvardsson Ragnar , Timsic Sandra , Harrison Ramona , Patterson William P. 
Published: June 2021

AbstractStable isotope analyses of zooarchaeological material can be used to examine ecological variability in exploited species at centennial to millennial scales. Climate change is a notable driver of marine ecosystem change, although historical fishing is also likely to have impacted past marine systems. Fishing removes the oldest and largest individuals and may thereby result in shorter trophic pathways and reduced niche width of predatory fish species. In the current study we examine the trophic niche of Atlantic cod, haddock and Atlantic wolffish, in the last millennium using δ13C and δ15N values of bone collagen. We report a lower trophic level of Atlantic cod and haddock but higher level of wolffish in present times, following centuries at consistent and higher trophic levels of Atlantic cod. This results in a concurrent converging trophic niche of the demersal fish. We suggest that the current data set provides a valuable historical baseline facilitating interpretation of current variability in the trophic ecology of northern demersal fish.
Functionalized MWCNTs-quartzite nanocomposite coated with Dacryodes edulis stem bark extract for the attenuation of hexavalent chromium
Authors: Amaku James F. , Ogundare Segun , Akpomie Kovo G. , Ibeji Collins U. , Conradie Jeanet 
Published: June 2021

AbstractMultiwalled carbon nanotubes/quartzite nanocomposite modified with the extract of Dacryodes edulis leaves was synthesized and designated as Q, which was applied for the removal of Cr(VI) from water. The adsorbents (PQ and Q) were characterized using the SEM, EDX, FTIR, TGA, XRD, and BET analyses. The XRD revealed the crystalline composition of the nanocomposite while the TGA indicated the incorporated extract as the primary component that degraded with an increase in temperature. The implication of the modifier was noticed to enhance the adsorption capacity of Q for Cr(VI) by the introduction of chemical functional groups. Optimum Cr(VI) removal was noticed at a pH of 2.0, adsorbent dose (50 mg), initial concentration (100 mg dm−3), and contact time (180 min). The kinetic adsorption data for both adsorbents was noticed to fit well to the pseudo-second-order model. The adsorption equilibrium data were best described by the Langmuir model. The uptake of Cr(VI) onto PQ and Q was feasible, endothermic (ΔH: PQ = 1.194 kJ mol−1 and Q = 34.64 kJ mol−1) and entropy-driven (ΔS : PQ = 64.89 J K−1 mol−1 and q = 189.7 J K−1 mol−1). Hence, the nanocomposite demonstrated potential for robust capacity to trap Cr(VI) from aqueous solution.
A DNA methylation-based liquid biopsy for triple-negative breast cancer
Authors: Cristall Katrina , Bidard Francois-Clement , Pierga Jean-Yves , Rauh Michael J. , Popova Tatiana , Sebbag Clara , Lantz Olivier , Stern Marc-Henri , Mueller Christopher R. 
Published: June 2021

AbstractHere, we present a next-generation sequencing (NGS) methylation-based blood test called methylation DETEction of Circulating Tumour DNA (mDETECT) designed for the optimal detection and monitoring of metastatic triple-negative breast cancer (TNBC). Based on a highly multiplexed targeted sequencing approach, this assay incorporates features that offer superior performance and included 53 amplicons from 47 regions. Analysis of a previously characterised cohort of women with metastatic TNBC with limited quantities of plasma (<2 ml) produced an AUC of 0.92 for detection of a tumour with a sensitivity of 76% for a specificity of 100%. mDETECTTNBC was quantitative and showed superior performance to an NGS TP53 mutation-based test carried out on the same patients and to the conventional CA15-3 biomarker. mDETECT also functioned well in serum samples from metastatic TNBC patients where it produced an AUC of 0.97 for detection of a tumour with a sensitivity of 93% for a specificity of 100%. An assay for BRCA1 promoter methylation was also incorporated into the mDETECT assay and functioned well but its clinical significance is currently unclear. Clonal Hematopoiesis of Indeterminate Potential was investigated as a source of background in control subjects but was not seen to be significant, though a link to adiposity may be relevant. The mDETECTTNBC assay is a liquid biopsy able to quantitatively detect all TNBC cancers and has the potential to improve the management of patients with this disease.
Brain network reconfiguration for narrative and argumentative thought
Authors: Xu Yangwen , Vignali Lorenzo , Collignon Olivier , Crepaldi Davide , Bottini Roberto 
Published: June 2021

AbstractOur brain constructs reality through narrative and argumentative thought. Some hypotheses argue that these two modes of cognitive functioning are irreducible, reflecting distinct mental operations underlain by separate neural bases; Others ascribe both to a unitary neural system dedicated to long-timescale information. We addressed this question by employing inter-subject measures to investigate the stimulus-induced neural responses when participants were listening to narrative and argumentative texts during fMRI. We found that following both kinds of texts enhanced functional couplings within the frontoparietal control system. However, while a narrative specifically implicated the default mode system, an argument specifically induced synchronization between the intraparietal sulcus in the frontoparietal control system and multiple perisylvian areas in the language system. Our findings reconcile the two hypotheses by revealing commonalities and differences between the narrative and the argumentative brain networks, showing how diverse mental activities arise from the segregation and integration of the existing brain systems.
Climate-driven divergence in plant-microbiome interactions generates range-wide variation in bud break phenology
Authors: Ware Ian M. , Van Nuland Michael E. , Yang Zamin K. , Schadt Christopher W. , Schweitzer Jennifer A. , Bailey Joseph K. 
Published: June 2021

AbstractSoil microbiomes are rapidly becoming known as an important driver of plant phenotypic variation and may mediate plant responses to environmental factors. However, integrating spatial scales relevant to climate change with plant intraspecific genetic variation and soil microbial ecology is difficult, making studies of broad inference rare. Here we hypothesize and show: 1) the degree to which tree genotypes condition their soil microbiomes varies by population across the geographic distribution of a widespread riparian tree, Populus angustifolia; 2) geographic dissimilarity in soil microbiomes among populations is influenced by both abiotic and biotic environmental variation; and 3) soil microbiomes that vary in response to abiotic and biotic factors can change plant foliar phenology. We show soil microbiomes respond to intraspecific variation at the tree genotype and population level, and geographic variation in soil characteristics and climate. Using a fully reciprocal plant population by soil location feedback experiment, we identified a climate-based soil microbiome effect that advanced and delayed bud break phenology by approximately 10 days. These results demonstrate a landscape-level feedback between tree populations and associated soil microbial communities and suggest soil microbes may play important roles in mediating and buffering bud break phenology with climate warming, with whole ecosystem implications.
Ultrafast electron energy-dependent delocalization dynamics in germanium selenide
Authors: Chen Zhesheng , Xiong Heqi , Zhang Hao , Gao Chaofeng , Cheng Yingchun , Papalazarou Evangelos , Perfetti Luca , Marsi Marino , Rueff Jean-Pascal 
Published: June 2021

AbstractUltrafast scattering process of high-energy carriers plays a key role in the performance of electronics and optoelectronics, and have been studied in several semiconductors. Core-hole clock spectroscopy is a unique technique for providing ultrafast charge transfer information with sub-femtosecond timescale. Here we demonstrate that germanium selenide (GeSe) semiconductor exhibits electronic states-dependent charge delocalization time by resonant photo exciting the core electrons to different final states using hard-x-ray photoemission spectroscopy. Thanks to the experiment geometry and the different orbital polarizations in the conduction band, the delocalization time of electron in high energy electronic state probed from Se 1s is ~470 as, which is three times longer than the delocalization time of electrons located in lower energy electronic state probed from Ge 1s. Our demonstration in GeSe offers an opportunity to precisely distinguish the energy-dependent dynamics in layered semiconductor, and will pave the way to design the ultrafast devices in the future.
Luminescent terpyridine appended geminal bisazide and bistriazoles: multinuclear Pt(ii) complexes and AIPE-based DNA detection with the naked eye
Authors: Das Bishnu , Gupta Parna 
Published: June 2021

Terpyridine appended stable gem-bistriazoles afford multinuclear platinum(ii) complexes upon reaction with Pt(dmso)2Cl2. Pt-complexes show excellent AIPE when interacts with ct-DNA. Naked eye DNA-detection is possible when exposed to 366 nm light.
Heparanase as a potential player in SARS-CoV-2 infection and induced coagulopathy
Authors: Kinaneh Safa , Khamaysi Iyad , Karram Tony , Hamoud Shadi 
Published: June 2021

Abstract
During the current formidable COVID-19 pandemic, it is appealing to address ideas that may invoke therapeutic interventions. Clotting disorders are well recognized in patients infected with severe acute respiratory syndrome (SARS) caused by a novel coronavirus (SARS-CoV-2), which lead to severe complications that worsen the prognosis in these subjects.
Increasing evidence implicate Heparan sulfate proteoglycans (HSPGs) and Heparanase in various diseases and pathologies, including hypercoagulability states. Moreover, HSPGs and Heparanase are involved in several viral infections, in which they enhance cell entry and release of the viruses.
Herein we discuss the molecular involvement of HSPGs and heparanase in SARS-CoV-2 infection, namely cell entry and release, and the accompanied coagulopathy complications, which assumedly could be blocked by heparanase inhibitors such as Heparin and Pixatimod.
Associations Between Language Difficulties, Peer Victimization, and Bully Perpetration From 3 Through 8 Years of Age: Results From a Population-Based Study
Authors: Øksendal Elise , Brandlistuen Ragnhild Eek , Wolke Dieter , Helland Siri Saugestad , Holte Arne , Wang Mari Vaage 
Published: June 2021

Background and PurposeSchoolchildren with language difficulties experience more peer victimization compared to their typically developing (TD) peers. Whether these children also bully their peers (bully perpetration) more than TD children is unclear. Furthermore, little is known about peer victimization and bully perpetration among preschool children with language difficulties and how it may be related to different paths of language difficulties. This study aimed to investigate associations between language difficulties, peer victimization, and bully perpetration from preschool to school age as well as the risk of peer victimization and bully perpetration for children with different developmental paths of language difficulties and mild language difficulties compared to TD children.MethodThe sample was drawn from the Norwegian Mother, Father and Child Cohort Study. Participants with completed questionnaires at 3, 5, and 8 years of age (n= 22,628) were included. Paths between latent variables of language skills at 3, 5, and 8 years of age, peer victimization at 5 and 8 years of age, and bully perpetration at 8 years of age were examined with structural equation modeling. Logistic regression was used to investigate peer victimization and bully perpetration for predefined paths of language difficulties.ResultsPoor language skills at 3 and 5 years of age were associated with peer victimization at 5 years of age. Poor language skills at 5 and 8 years of age were associated with peer victimization and bully perpetration at 8 years of age. The association between poor language skills at 5 years of age and bully perpetration at 8 years of age was stronger for girls. Persistent paths of language difficulties at 3, 5, and 8 years of age showed the highest risk of peer victimization and bully perpetration.ConclusionsLanguage difficulties are associated with peer victimization and bully perpetration. The risk of peer victimization and bully perpetration differs according to different developmental paths of language difficulties from preschool to school age.