bluuebunny/crossref_metadata_2025_split huggingface dataset:
Co-inoculation of rhizobacteria promotes growth, yield, and nutrient contents in soybean and improves soil enzymes and nutrients under drought conditions
Authors: Jabborova Dilfuza , Kannepalli Annapurna , Davranov Kakhramon , Narimanov Abdujalil , Enakiev Yuriy , Syed Asad , Elgorban Abdallah M. , Bahkali Ali H. , Wirth Stephan , Sayyed R. Z. , Gafur Abdul 
Published: November 2021

AbstractDrought stress is the major abiotic factor limiting crop production. Co-inoculating crops with nitrogen fixing bacteria and plant growth-promoting rhizobacteria (PGPR) improves plant growth and increases drought tolerance in arid or semiarid areas. Soybean is a major source of high-quality protein and oil for humans. It is susceptible to drought stress conditions. The co-inoculation of drought-stressed soybean with nodulating rhizobia and root-colonizing, PGPR improves the root and the shoot growth, formation of nodules, and nitrogen fixation capacity in soybean. The present study was aimed to observe if the co-inoculation of soybean (Glycine max L. (Merr.) nodulating with Bradyrhizobium japonicum USDA110 and PGPR Pseudomonas putida NUU8 can enhance drought tolerance, nodulation, plant growth, and nutrient uptake under drought conditions. The results of the study showed that co-inoculation with B. japonicum USDA110 and P. putida NUU8 gave more benefits in nodulation and growth of soybean compared to plants inoculated with B. japonicum USDA110 alone and uninoculated control. Under drought conditions, co-inoculation of B. japonicum USDA 110 and P. putida NUU8 significantly enhanced the root length by 56%, shoot length by 33%, root dry weight by 47%, shoot dry weight by 48%, and nodule number 17% compared to the control under drought-stressed. Co-inoculation with B. japonicum, USDA 110 and P. putida NUU8 significantly enhanced plant and soil nutrients and soil enzymes compared to control under normal and drought stress conditions. The synergistic use of B. japonicum USDA110 and P. putida NUU8 improves plant growth and nodulation of soybean under drought stress conditions. The results suggested that these strains could be used to formulate a consortium of biofertilizers for sustainable production of soybean under drought-stressed field conditions.
A global meta-analysis of yield and water productivity responses of vegetables to deficit irrigation
Authors: Singh Manpreet , Singh Paramveer , Singh Sukhbir , Saini Rupinder Kaur , Angadi Sangamesh V. 
Published: November 2021

AbstractStrategies promoting efficient water use and conserving irrigation water are needed to attain water security to meet growing food demands. This meta-analysis study evaluated the effect of deficit irrigation (DI) strategy on eight vegetables to provide a quantitative estimate of yield and water productivity (WP) responses under variable soil textures, climates, and production systems (open-field and greenhouse). This study analyzed 425 yield and 388 WP comparisons of different DI levels to full irrigation (FI), extracted from 185 published studies representing 30 countries. Moving from the highest (> 80%FI) to the lowest (< 35%FI) irrigation level, the overall yield decline was 6.9 to 51.1% compared to FI, respectively. The WP gains ranged from 8.1 to 30.1%, with 35–50%FI recording the highest benefits. Soil texture affected the yield significantly only under the least irrigation class (< 35%FI), wherein sandy clay and loam recorded the highest (82.1%) and the lowest (26.9%) yield decline, respectively. Among the climates, temperate climate was overall the most advantageous with the least yield penalty (21.9%) and the highest WP gain (21.78%) across various DI levels. The DI application under the greenhouse caused lesser yield reduction compared to the open-field. The WP gains due to DI were also higher for greenhouse (18.4%) than open-field (13.6%). Consideration of yield penalties and the cost of saved irrigation water is crucial while devising the reduced irrigation amounts to the crops. The yield reductions under low to moderate water deficits (> 65%FI) accompanied by gains in WP may be justifiable in the light of anticipated water restriction.
Spontaneous pulmonary emphysema in mice lacking all three nitric oxide synthase isoforms
Authors: Kato Kaori , Tsutsui Masato , Noguchi Shingo , Iha Yukitoshi , Naito Keisuke , Ogoshi Takaaki , Nishida Chinatsu , Tahara Masahiro , Yamashita Hirotaka , Wang Ke-Yong , Toyohira Yumiko , Yanagihara Nobuyuki , Masuzaki Hiroaki , Shimokawa Hiroaki , Tanimoto Akihide , Yatera Kazuhiro 
Published: November 2021

AbstractThe roles of endogenous nitric oxide (NO) derived from the entire NO synthases (NOSs) system have yet to be fully elucidated. We addressed this issue in mice in which all three NOS isoforms were deleted. Under basal conditions, the triple n/i/eNOSs−/− mice displayed significantly longer mean alveolar linear intercept length, increased alveolar destructive index, reduced lung elastic fiber content, lower lung field computed tomographic value, and greater end-expiratory lung volume as compared with wild-type (WT) mice. None of single NOS−/− or double NOSs−/− genotypes showed such features. These findings were observed in the triple n/i/eNOSs−/− mice as early as 4 weeks after birth. Cyclopaedic and quantitative comparisons of mRNA expression levels between the lungs of WT and triple n/i/eNOSs−/− mice by cap analysis of gene expression (CAGE) revealed that mRNA expression levels of three Wnt ligands and ten Wnt/β-catenin signaling components were significantly reduced in the lungs of triple n/i/eNOSs−/− mice. These results provide the first direct evidence that complete disruption of all three NOS genes results in spontaneous pulmonary emphysema in juvenile mice in vivo possibly through down-regulation of the Wnt/β-catenin signaling pathway, demonstrating a novel preventive role of the endogenous NO/NOS system in the occurrence of pulmonary emphysema.
Method for the biomechanical analysis of aqueous veins and perilimbal sclera by three-dimensional photoacoustic imaging and strain field calculation
Authors: Ni Linyu , Riesterer John , Wang Huaizhou , Berry Layla , Blackburn Kara , Chuang Jonathan , Kim Wonsuk , Xu Guan , Moroi Sayoko E. , Argento Alan 
Published: November 2021

AbstractA method motivated by the eye’s aqueous veins is described for the imaging and strain calculation within soft biological tissues. A challenge to the investigation of the biomechanics of the aqueous vein—perilimbal sclera tissue complex is resolution of tissue deformations as a function of intraocular pressure and the subsequent calculation of strain (a normalized measure of deformation). The method involves perfusion of the eye with a contrast agent during conduction of non-invasive, optical resolution photoacoustic microscopy. This imaging technique permits three-dimensional displacement measurements of tracked points on the inner walls of the veins which are used in a finite element model to determine the corresponding strains. The methods are validated against two standard strain measurement methods. Representative porcine globe perfusion experiments are presented that demonstrate the power of the method to determine complex strain fields in the veins dependent on intraocular pressure as well as vein anatomy. In these cases, veins are observed to move radially outward during increases in intraocular pressure and to possess significant spatial strain variation, possibly influenced by their branching patterns. To the authors’ knowledge, these are the only such quantitative, data driven, calculations of the aqueous vein strains available in the open literature.
Effects of competitive pressure and habitat heterogeneity on niche partitioning between Arctic and boreal congeners
Authors: Bonnet-Lebrun Anne-Sophie , Larsen Thomas , Frederiksen Morten , Fox Derren , le Bouard Fabrice , Boutet Aude , Þórarinsson Þorkell Lindberg , Kolbeinsson Yann , Deville Tanguy , Ratcliffe Norman 
Published: November 2021

AbstractThe rapidly changing climate in the Arctic is expected to have a major impact on the foraging ecology of seabirds, owing to changes in the distribution and abundance of their prey but also that of competitors (e.g. southerly species expanding their range into the Arctic). Species can respond to interspecific competition by segregating along different niche axes. Here, we studied spatial, temporal and habitat segregation between two closely related seabird species: common guillemot Uria aalge (a temperate species) and Brünnich’s guillemot Uria lomvia (a true Arctic species), at two sympatric sites in Iceland that differ in their total population sizes and the availability of marine habitats. We deployed GPS and temperature-depth recorders to describe foraging locations and behaviour of incubating and chick-rearing adults. We found similar evidence of spatial segregation at the two sites (i.e. independent of population sizes), although segregation in environmental space was only evident at the site with a strong habitat gradient. Unexpectedly, temporal (and, to a limited extent, vertical) segregation appeared only at the least populated site. Overall, our results show complex relationships between the levels of inferred competition and that of segregation.
Standard 6-week chemoradiation for elderly patients with newly diagnosed glioblastoma
Authors: Vaugier Loïg , Ah-Thiane Loïc , Aumont Maud , Jouglar Emmanuel , Campone Mario , Colliard Camille , Doucet Ludovic , Frenel Jean-Sébastien , Gourmelon Carole , Robert Marie , Martin Stéphane-André , Riem Tanguy , Roualdes Vincent , Campion Loïc , Mervoyer Augustin 
Published: November 2021

AbstractGlioblastoma (GBM) is frequent in elderly patients, but their frailty provokes debate regarding optimal treatment in general, and the standard 6-week chemoradiation (CRT) in particular, although this is the mainstay for younger patients. All patients with newly diagnosed GBM and age ≥ 70 who were referred to our institution for 6-week CRT were reviewed from 2004 to 2018. MGMT status was not available for treatment decision at that time. The primary endpoint was overall survival (OS). Secondary outcomes were progression-free survival (PFS), early adverse neurological events without neurological progression ≤ 1 month after CRT and temozolomide hematologic toxicity assessed by CTCAE v5. 128 patients were included. The median age was 74.1 (IQR: 72–77). 15% of patients were ≥ 80 years. 62.5% and 37.5% of patients fulfilled the criteria for RPA class I–II and III–IV, respectively. 81% of patients received the entire CRT and 28% completed the maintenance temozolomide. With median follow-up of 11.7 months (IQR: 6.5–17.5), median OS was 11.7 months (CI 95%: 10–13 months). Median PFS was 9.5 months (CI 95%: 9–10.5 months). 8% of patients experienced grade ≥ 3 hematologic events. 52.5% of patients without neurological progression had early adverse neurological events. Post-operative neurological disabilities and age ≥ 80 were not associated with worsened outcomes. 6-week chemoradiation was feasible for “real-life” elderly patients diagnosed with glioblastoma, even in the case of post-operative neurological disabilities. Old does not necessarily mean worse.
Honey bee sHSP are responsive to diverse proteostatic stresses and potentially promising biomarkers of honey bee stress
Authors: Shih Samantha R. , Bach Dunay M. , Rondeau Nicole C. , Sam Jessica , Lovinger Natalie L. , Lopatkin Allison J. , Snow Jonathan W. 
Published: November 2021

AbstractThe pollination services provided by the honey bee are critical in both natural and agricultural ecosystems. Honey bee colonies in the United States have suffered from an increased rate of die-off in recent years, stemming from a complex set of interacting stresses that remain poorly described. Defining specific common cellular processes and cellular stress responses impacted by multiple stressors represent a key step in understanding these synergies. Proteotoxic stresses negatively impact protein synthesis, folding, and degradation. Diverse proteotoxic stresses induce expression of genes encoding small heat shock proteins (sHSP) of the expanded lethal (2) essential for life (l(2)efl) gene family. In addition to upregulation by the Integrated Stress Response (ISR), the Heat Shock Response (HSR), and the Oxidative Stress Response (OSR), our data provide first evidence that sHSP genes are upregulated by the Unfolded Protein Response (UPR). As these genes appear to be part of a core stress response that could serve as a useful biomarker for cellular stress in honey bees, we designed and tested an RT-LAMP assay to detect increased l(2)efl gene expression in response to heat-stress. While this assay provides a powerful proof of principle, further work will be necessary to link changes in sHSP gene expression to colony-level outcomes, to adapt our preliminary assay into a Point of Care Testing (POCT) assay appropriate for use as a diagnostic tool for use in the field, and to couple assay results to management recommendations.
C-reactive protein as an early biomarker for malaria infection and monitoring of malaria severity: a meta-analysis
Authors: Wilairatana Polrat , Mahannop Praphassorn , Tussato Thanita , Hayeedoloh I-mee , Boonhok Rachasak , Klangbud Wiyada Kwanhian , Mala Wanida , Kotepui Kwuntida Uthaisar , Kotepui Manas 
Published: November 2021

AbstractThis study investigated whether C-reactive protein (CRP) can be used as a marker for the early detection and monitoring of malaria severity. Potentially relevant studies were searched in Medline (PubMed), Scopus, and Web of Science. Differences in CRP between (1) severe malaria and uncomplicated malaria, (2) uncomplicated malaria and asymptomatic malaria, (3) uncomplicated malaria and febrile/healthy controls, and (4) asymptomatic malaria and febrile/healthy controls were estimated using random-effects models. Twenty-nine studies were included for meta-analysis. The results of meta-analysis demonstrated higher mean CRP levels in (1) patients with severe malaria compared with uncomplicated malaria (p < 0.001, standard mean difference [SMD]: 1.52, 95% confidence interval [CI]: 0.91–2.12, I2: 95.1%), (2) patients with uncomplicated malaria than in those with asymptomatic malaria (p: 0.001, SMD: 1.65, 95% CI: 0.67–2.62, I2: 96.7%), (3) patients with uncomplicated malaria compared with febrile/healthy controls (p < 0.001, SMD: 2.38, 95% CI: 1.37–3.40, I2: 98.5%), and (4) patients with asymptomatic malaria compared with febrile/healthy controls (p < 0.001, SMD: 2.55, 95% CI: 1.60–3.50, I2: 99.2%). This study demonstrated CRP levels are a biomarker for the early detection and monitoring of malaria severity.
Pitting of malaria parasites in microfluidic devices mimicking spleen interendothelial slits
Authors: Elizalde-Torrent Aleix , Trejo-Soto Claudia , Méndez-Mora Lourdes , Nicolau Marc , Ezama Oihane , Gualdrón-López Melisa , Fernández-Becerra Carmen , Alarcón Tomás , Hernández-Machado Aurora , del Portillo Hernando A. 
Published: November 2021

AbstractThe spleen is a hematopoietic organ that participates in cellular and humoral immunity. It also serves as a quality control mechanism for removing senescent and/or poorly deformable red blood cells (RBCs) from circulation. Pitting is a specialized process by which the spleen extracts particles, including malaria parasites, from within circulating RBCs during their passage through the interendothelial slits (IES) in the splenic cords. To study this physiological function in vitro, we have developed two microfluidic devices modeling the IES, according to the hypothesis that at a certain range of mechanical stress on the RBC, regulated through both slit size and blood flow, would force it undergo the pitting process without affecting the cell integrity. To prove its functionality in replicating pitting of malaria parasites, we have performed a characterization of P. falciparum-infected RBCs (P.f.-RBCs) after their passage through the devices, determining hemolysis and the proportion of once-infected RBCs (O-iRBCs), defined by the presence of a parasite antigen and absence of DAPI staining of parasite DNA using a flow cytometry-based approach. The passage of P.f.-RBCs through the devices at the physiological flow rate did not affect cell integrity and resulted in an increase of the frequency of O-iRBCs. Both microfluidic device models were capable to replicate the pitting of P.f.-RBCs ex vivo by means of mechanical constraints without cellular involvement, shedding new insights on the role of the spleen in the pathophysiology of malaria.
Nanoengineering of eco-friendly silver nanoparticles using five different plant extracts and development of cost-effective phenol nanosensor
Authors: Jebril Siwar , Fdhila Alaeddine , Dridi Chérif 
Published: November 2021

AbstractThe production of environmentally friendly silver nanoparticles (AgNPs) has aroused the interest of the scientific community due to their wide applications mainly in the field of environmental pollution detection and water quality monitoring. Here, for the first time, five plant leaf extracts were used for the synthesis of AgNPs such as Basil, Geranium, Eucalyptus, Melia, and Ruta by a simple and eco-friendly method. Stable AgNPs were obtained by adding a silver nitrate (AgNO3) solution with the leaves extract as reducers, stabilizers and cappers. Only, within ten minutes of reaction, the yellow mixture changed to brown due to the reduction of Ag+ ions to Ag atoms. The optical, structural, and morphology characteristics of synthesized AgNPs were determined using a full technique like UV–visible spectroscopy, FTIR spectrum, XRD, EDX spectroscopy, and the SEM. Thus, Melia azedarach was found to exhibit smaller nanoparticles (AgNPs-M), which would be interesting for electrochemical application. So, a highly sensitive electrochemical sensor based on AgNPs-M modified GCE for phenol determination in water samples was developed, indicating that the AgNPs-M displayed good electrocatalytic activity. The developed sensor showed good sensing performances: a high sensitivity, a low LOD of 0.42 µM and good stability with a lifetime of about one month, as well as a good selectivity towards BPA and CC (with a deviation less than 10%) especially for nanoplastics analysis in the water contained in plastics bottles. The obtained results are repeatable and reproducible with RSDs of 5.49% and 3.18% respectively. Besides, our developed sensor was successfully applied for the determination of phenol in tap and mineral water samples. The proposed new approach is highly recommended to develop a simple, cost effective, ecofriendly, and highly sensitive sensor for the electrochemical detection of phenol which can further broaden the applications of green silver NPs.
Comparison of nasal patency after nose-blowing between pinch versus no pinch method: a randomized controlled trial
Authors: Piromchai Patorn , Netnoi Jakkrit , Srirompotong Supaporn , Thanawirattananit Panida 
Published: November 2021

AbstractWe proposed that nose-blowing without pinching was safer and able to get rid of mucus and maintain nasal patency as effective as the pinch and blow method. The objective of this study was to evaluate the nasal patency after nose-blowing by pinching the nose versus no pinching. The patients who have nasal discharge such as allergic rhinitis or common cold were recruited. The patients were randomized to perform pinching or no pinching nose-blowing. Fifty patients were enrolled in this study. The objective evaluation using acoustic rhinometry found no difference in nasal patency between the two groups (p > 0.05). The subjective patency score was significantly higher in the pinch one nostril shut group (mean difference 0.88, 95% CI 0.20–1.55). The patency of the two methods were comparable according to the objective test. However, the patients felt that their nose was clearer when pinching and blowing.
Silicon and mechanical damage increase polyphenols and vitexin in Passiflora incarnata L.
Authors: Pagassini Jonas Akenaton Venturineli , de Godoy Leandro José Grava , Campos Felipe Girotto , Barzotto Gustavo Ribeiro , Vieira Maria Aparecida Ribeiro , Boaro Carmen Sílvia Fernandes 
Published: November 2021

AbstractPassiflora incarnata L. is a species of global pharmacological importance, has not been fully studied in the context of cultivation and management. It is known that silicon acts on abiotic stress and promotes phenols synthesis. The practice of mechanical damage is widely used in P. incarnata crops, and its interaction with silicon can have a significant influence on plant metabolism. Therefore, our objective was to investigate the effects of silicon and mechanical damage on photosynthesis, polyphenols and vitexin of P. incarnata. The experiment was conducted in a factorial design with SiO2 concentrations (0, 1, 2, 3 mM) and presence or absence of mechanical damage. It was found that mechanical damage improved photosynthetic performance at lower concentrations or absence of silicon. Moreover, this condition promoted an increasing in vitexin concentration when SiO2 was not provided. The application of 3 mM Si is recommended to increase polyphenols and vitexin, without harming dry mass of aerial part. The interaction between silicon and mechanical damage could be a tool to increase agronomic yield and commercial value of the P. incarnata crop.
Effects of interactions in natural gas/water/rock system on hydrocarbon migration and accumulation
Authors: Jiang Lin , Zhao Wen , Huang Jianguo , Fan Yang , Hao Jiaqing 
Published: November 2021

AbstractThe study of natural gas accumulation process in tight formation has become the focus of the petroleum industry. One of the priorities is the effects of interactions in natural gas/water/rock system on hydrocarbon migration and accumulation process. On the macroscopic scale, we investigate the interactions in natural gas/water/rock system by formation fluorescence test and production data analysis. One the microscopic scale, the mechanisms are revealed by mathematical analysis and experimental methods considering the variation of geological temperature and pressure. The effects of interactions in natural gas/water/rock system are also simulated by numerical simulation. The results are visualized and quantified. A novel semi-analytical method based on a physical experiment is proposed to calculate the temperature- and pressure-dependent contact angle and interface tension which reflect the interactions in the natural gas–water–rock system. This semi-analytical is embedded in the numerical simulation during the simulation of the natural gas charging process. The results indicate that with the increase of geological temperature and pressure, the contact angle will increase and the interface tension between natural gas and water will decrease. The capillary resistance in the formation will be reduced. Since the decrease of capillary resistance, the natural gas can be charged into smaller pores, so that the actual charging threshold is lower than the one originally obtained under present reservoir conditions. After considering the temperature and pressure during the accumulation process, some sand bodies that were thought not to be charged may have natural gas accumulate.