bluuebunny/crossref_metadata_2025_split huggingface dataset:
Global Identification of C2 Domain Contain Proteins And Characterization Of Genetic Variations For Involved Abiotic Stress Tolerance In Rice (Oryza sativa L.)
Authors: Zhang Hongjia , Zeng Yuting , Seo Jeonghwan , Kim Yu-Jin , Kim Sun Tae , Kwon Soon-Wook 
Published: July 2021

Abstract
BackgroundC2DPs (C2 domain contain proteins) have been identified in different genomes that contain single or multiple C2 domains in their C or N-terminal, it possesses higher functional activity in the cell membrane between the cytoplasm and nucleus. Despite the identification of MCTPs and NTMC2s in rice, Arabidopsis, and cotton in a previous study, however, the C2DP gene family in rice has not been comprehensively studied, and the role of the C2DP gene in rice in response to abiotic stress is unclear.ResultsIn this study, we identified 82 C2DPs in the rice genome and divided them into seven groups through phylogenetic analysis. Synteny analysis revealed that duplication events were either exhibited within the genome of rice or between the genome of rice and other species. Through the analysis of cis-acting elements in promoters, expression profiles, and qRT-PCR results, the functions of OsC2DPs were found to be widely expressed in diverse tissues and were extensively involved in phytohormones and abiotic stress in rice. Prediction of the miRNA targets of OsC2DPs revealed that some of the homolog genes were regulated by consistent miRNAs and may carry out redundancy function. Notably, OsC2DP50/51/52 as a co-tandem duplication exhibited similar expression variations and involved the coincident miRNA-regulation pathway. Moreover, the results of SNP genotyping and haplotype analysis revealed that OsC2DP17, OsC2DP29, and OsC2DP49 possessed diverse haplotypes for impacted cold tolerance owing to genomic variations.ConclusionsThese findings provide a comprehensive sight for characterized OsC2DPs in rice and their roles for abiotic stress. Further, the genetic variation supports the theoretical reference for molecular breeding in rice.
Diagnostic Performance of MRI, SPECT, and PET in Detecting Renal Cell Carcinoma: A Meta-Analysis
Authors: Yin Qihua , Xu Huiting , Zhong Yanqi , Ni Jianming , Hu Shudong 
Published: July 2021

Abstract
Background: Renal cell carcinoma (RCC) is one of the most common malignancies worldwide. Noninvasive imaging techniques, such as magnetic resonance imaging (MRI), single photon emission computed tomography (SPECT), and positron emission tomography (PET), have been involved in increasing evolution to detect RCC. This meta-analysis aims to compare to compare the value of MRI, SPECT, and PET in the diagnosis of RCC, and to provide evidence for decision-making in terms of further research and clinical settings.Methods: Electronic databases including PubMed, Web of Science, Embase, and Cochrane Library were systemically searched. Studies concerning MRI, SPECT, and PET for the detection of RCC were included. Pooled sensitivity, specificity, positive likelihood ratio (PLR), negative likelihood ratio (NLR), diagnostic odds ratio (DOR), with their respective 95% confidence interval (CIs) and the area under the summary receiver operating characteristic (SROC) curve (AUC) were calculated.Results: A total of 44 articles were finally detected for inclusion in this meta-analysis. The pooled sensitivities of MRI, SPECT, and PET were 0.80, 0.81, and 0.88, respectively. Their respective overall specificities were 0.90, 0.54, and 0.87. Results in the subgroup analysis of the performance of SPECT that the pooled sensitivity, specificity, and AUC of the prospective SPECT studies included were 0.80, 0.42, 0.80, respectively. In the analysis of 18F-FDG PET, the pooled sensitivity, specificity, and AUC were 0.88, 0.86, and 0.92, respectively. PET studies showed a pooled sensitivity, specificity, and AUC of 0.80, 0.85, and 0.85, respectively in the diagnosis of primary RCC. The pooled sensitivity, specificity, and AUC of PET studies in detecting recurrent or metastatic RCC were 0.93, 0.88, and 0.94.Conclusion: Our meta-analysis manifests that MRI and PET present better diagnostic value for the detection of RCC in comparison with SPECT. PET is superior in the diagnosis of recurrent or metastatic RCC.
Evaluating the Multifunctional Resilience of River Health to Human Service Demand: A Comparison among Fuzzy Logic, Entropy and AHP Based MCDM Models
Authors: BHATTACHARYA RAJ , Chatterjee Nilanjana Das , Das Kousik 
Published: July 2021

Abstract
Ecosystem services of river for human beings are guided by harmonious relationship; however over growing human service demands (HSDs) are leading to deteriorate the river health connotation. In this study, an assessment index system of monsoonal quarried river health including twenty indicators consisting of riparian, morphological, hydroecological and social structure were established to detect the multifunctional threshold of river system integrity in respect to HSDs at upper (US), middle (MS), lower segments (LS) of Kangsabati River using fuzzy logic, AHP and entropy based multi-criteria decision matrix (MCDM) methods i.e. vlseKriterijumska optimizacija I Kompromisno Resenje (VIKOR), simple additive weighing (SAW), complex proportion assessment of alternatives (COPRAS), weighted aggregated sum product assessment (WASPAS), technique for order preference by similarity to ideal solution (TOPSIS). The results revealed that overall indicators performance is generally healthy in MS, medium health level in US but mostly unhealthy in LS; and AHP and fuzzy MCDM methods assigned as high priority rank to MS, medium rank to US and least rank to LS, while entropy MCDM methods gives highest rank to US, medium rank to MS and least rank to LS, respectively. According to model validation performances, fuzzy and AHP MCDM methods are signified to HSDs, and results are closer to real problems, whereas entropy MCDM methods are rationalized to harmonic relationship of riverine system. With the acceptability of AHP SAW and WASPASS, it can be concluded that over HSDs is main culprit for river health degradation, and research results are identified the sick sites for ecological restoration.
Evaluation of Qualitative Traits In Modified Iranian Red Rey Onion And Comparison Genetic Resistance With Primary Mass Selection And Red Azar-Shahr cv To Fusarium Oxysporum Using Laboratory And Molecular Markers
Authors: Shokrgozar Sayyedeh Mahsan Taghi , Khodadadi Mohsen , Abdossi Vahid , nia Vahid Zarrin , far Ramin Hajiyan 
Published: July 2021

Abstract
The aim of this study is to evaluate the storage and qualitative feature of modified Red Rey Iranian Onion. Firstly, the modified population was obtained by the selection of superior bulbs, cultivation, its self-pollination and consequently the identification of the best families and implement open pollination between them. In next level, the Red Rey Iranian modified with basic population and Red Azar-shahr cultivar (comparative) was crossed. Our results showed that the selection procedure has leading to improvement in variety of traits in population. Also, the modified Red Rey is significantly superior to the base mass in qualitative traits such as: bulb stiffness, bulb dry matter, TSS, total sugar and glucose; So that the percentage of dry bulb content increased from 10.4% in the basal mass to 11.1% in the modified Red Rey; while spouring and rotting, minerals, and dry matter, vitamin C and fructose-reducing sugar was not affected by genotype. In the second step, resistances to Fusarium wilt disease (laboratory and molecular markers) were evaluated. Based on the results of phenotypic evaluation, the modified Red Rey had the lowest rate and level of infection and the highest score. According to the results of genotypic evaluation, there is a very high genetic affinity between resistant and susceptible cultivars.
Study on Flexible Polishing Force and Polishing Temperature of Flap Disc
Authors: Liu De , Pan Xiaoming , Gu Zhiyang , Qiu Hui 
Published: July 2021

Abstract
Polishing determines the final surface quality of the aero engine, which have great influence on its working performance and working life. By analyzing the structure and working principle of the flexible self-adaptive polishing platform of the blisk, the abrasive cutting model of the flap disc is established. The theoretical calculation of the effect of elastic deformation during the polishing process on the contact length of flap disc and blisk. The model of polishing force, polishing heat and temperature field during the polishing process of the flap disc are established and analyzed. Single factor method is used to analyze the influence of process parameters on polishing force, polishing temperature, roughness and specific polishing energy. Finally, the polishing test shows that the optimized process parameters improve the polished surface quality and meet the requirements of the blade polishing process.
Development and Verification of an IoT-Enabled Air Quality Monitoring System Based on Petri Nets
Authors: Lin Yi-Nan , Wang Sheng-Kuan , Chiou Gwo-Jen , Yang Cheng-Ying , Shen Victor R. L. , Su Zhi Yang 
Published: July 2021

Abstract
An IoT (Internet of Things) system for monitoring campus environmental parameters was successfully developed in this study. Various sensors were adopted in the front of IoT system for sensing air pollutants. The architecture of LongRange (LoRa) in Class C uses low-power and long-distance transmission technologies, which has been set up on the large campus, so that the terminal equipment can reach a balance between downlink latency and battery life, making it the best transmission communication protocol. In addition, this monitoring system uses the Petri net software tool to build a correct IoT platform based on the fundamental working processes, and to demonstrate the feasibility of the IoT system through simulation-based verification. Finally, the experimental results have shown that the IoT system for monitoring the campus environmental parameters achieves the goal of an acceptable data transmission success rate of more than 95%. Thus, it can facilitate the air quality trends for policy making as well as the hazardous prediction and prevention.
Peptide formation as on the early Earth: from amino acid mixtures to peptides in sulphur dioxide
Authors: Trapp Oliver , Sauer Fabian , Haas Maren , Sydow Constanze , Siegle Alexander , Lauer Christoph 
Published: July 2021

Abstract
The formation of peptide bonds is one of the most important biochemical reaction steps. Without the development of structurally and catalytically active polymers, there would be no life on our planet. Intensive research is being conducted on possible reaction pathways for the formation of complex peptides on the early Earth. Salt-induced peptide formation (SIPF) by metal catalysis is one possible pathway for abiotic peptide synthesis. The high salt concentration supports dehydration in this process. However, the formation of large, complex oligomer systems is prevented by the high thermodynamic barrier of peptide condensation in aqueous solution. Liquid sulphur dioxide proves to be a superior alternative for copper-catalysed peptide condensation. Compared to water, the amino acids are activated in sulphur dioxide, which leads to the incorporation of all 20 proteinogenic amino acids into the resulting proteins and thus to a large variety of products. Strikingly, even extremely low initial reactant concentrations of only 50 mM are sufficient for extensive peptide formation, leading to an overall yield of 2.9% for dialanine in 7 days. The reactions carried out at room temperature and the successful use of the Hadean mineral covellite as a catalyst, suggest a volcanic environment for the formation of the peptide world on early Earth as a likely scenario.
Protective Effects of Taurine Against Inflammation and Apoptosis in Cadmium-Induced Hepatotoxicity
Authors: Zheng Jiaming , Qiu Guobin , Zhou Yewen , Ma Kezhe , Cui Sheng 
Published: July 2021

Abstract
Cadmium (Cd), a heavy metal contaminant,which seriously threatens human and animal health. Taurine (Tau) has been used to against hepatotoxicity caused by different environmental toxins. However, it has not been elucidated whether Tau exerts its protective function against Cd-induced hepatotoxicity. The aim of this study was thus to evaluate the ameliorative function of Tau on Cd-induced liver toxicity in mice. The histopathologic and ultrastructure changes, as well as alterations in indexes related to liver function, antioxidant biomarkers, inflammatory and apoptosis were evaluated. The results showed that Tau alleviated the vacuolar degeneration, nuclear condensation, mitochondria swelling and cristae lysis of hepatocytes induced by Cd. In addition, Tau treatment significantly restored the ALT, AST levels in serum, and inflammatory factor TNF-α and IL-1β in liver tissue. Furthermore, Tau treatment decreased the Bax/Bcl-2 ratio and cleaved caspase-3 protein expression levels. Taken together, these observations demonstrate that Tau has important hepatic protective function against the inflammation and apoptosis induced by Cd.
Renewable Energy Consumption and Local Environmental Effects for Economic Growth: The Role of Financial Development and Foreign Direct Investment
Authors: khan Itbar , han lei , khan Hayat 
Published: July 2021

Abstract
The use of renewable energy improves environmental quality by reducing carbon emission and influence economics growth where carbon emission also effect economic growth of a country. The economic theory of tourism also indicates that tourism development enhance economic growth though spillovers as well contribute to climate change. The inflow of FDI and financial development enhance economic growth however its also effect environmental quality. Based on the ongoing debate, the present research trying attempts to explore the effect of CO2 emission and renewable energy consumption, FDI and financial development on economic growth in different income grouped countries to know whether these impacts are the same for the low income, middle income and high income countries on economic growth? Using panel data for high income, low income & middle income countries for the period of 1980–2018, the current study found that all variables effect economic growth significantly where FDI and carbon emission are positive while renewable energy consumption and financial development are negative for economic growth in the whole sample while its differ in the income groups. These studies have shown that these variables are not the same as the economic growth of economic growth and different income groups are not the same, but it changes. In addition, the foundation of this study has a great deal of recommendations for income Group economic decision make-up.
CD34 is a target for covalent EGFR inhibitors to eliminate stem/progenitor cells in acute and chronic myeloid leukemia
Authors: Lu Ying , Xia Li , Yang Li-Xue , Liu Hong-Chen , Jiang Yue , Kang Zhi-Jie , Zhang Cheng-Tao , Gao Bei-Bei , Zhang Xuehong , Waller Edmund , Yan Jinsong 
Published: July 2021

Abstract
The effect of epidermal growth factor receptor (EGFR) inhibitors on acute myeloid leukemia (AML) was discovered over one decade ago. However, clinical trials of EGFR inhibitors in AML have yielded controversial outcomes. Leukemia cells lack EGFR expression, and the mechanism by which EGFR inhibitors affect leukemia cell growth is unknown, obscuring the precise subset of AML patients that might be targeted by these compounds. Since myeloid leukemia arises from malignant stem/progenitors, here we evaluated the effect of EGFR inhibitors on primary leukemia stem/progenitors that expressed the stem cell marker CD34 which were sorted from leukemia patients. EGFR inhibitors induced significant apoptosis of primary CD34+ but not CD34− cells derived from AML and chronic myeloid leukemia (CML) patients both in vitro and in patient-derived xenotransplantation model. Using two EGFR inhibitors osimertinib and afatinib, we demonstrated binding and covalent adducts of the inhibitors with the cysteine(C) 199 residue of the CD34 protein, which downregulated phosphorylation of tyrosine 329(Y329) of CD34, leading to the dissociation of CD34 from tyrosine kinase Src and thereafter the inhibition of STAT3 phosphorylation. Most importantly, administration of osimertinib yielded clinical responses in two CD34-high AML patients identified by quantitative proteomics with reduced levels of Y329 phosphorylation of CD34 after treatment. Collectively, these findings delineate a novel molecular pathway whereby EGFR inhibitors kill leukemia and reveal that the CD34 antigen is a targetable signaling molecule that mediates cell survival signals via connecting to Src-STAT3 pathway.
Target deconvolution of HDAC pharmacopoeia highlights MBLAC2 as common off-target
Authors: Lechner Severin , Malgapo Martin , Grätz Christian , Baron Agnes , Rüther Patrick Leopold , Nadal Simon , Stumpf Carmen , Loos Christina , Ku Xin , Prokofeva Polina , Lautenbacher Ludwig , Heimburg Tino , Würf Vivian , Meng Chen , Sippl Wolfgang , Wilhelm Mathias , Kleigrewe Karin , Pauling Josch , Kramer Karl , Pfaffl Michael , Linder Maurine , Kuster Bernhard , Médard Guillaume 
Published: July 2021

Abstract
HDAC drugs have entered the pharmacopoeia in the 2000s. However, some enigmatic phenotypes suggest off-target engagement. Here, we developed a chemical proteomics assay using three promiscuous chemotypes and quantitative mass spectrometry that we deployed to establish the target landscape of 53 drugs. The results highlight 14 direct targets, including 9 out of the 11 human zinc-dependent HDACs, question the reported selectivity of widely-used molecules, notably for HDAC6, and delineate how the composition of HDAC complexes influences drug potency. Unexpectedly, metallo-beta-lactamase domain-containing protein 2 (MBLAC2) featured as a frequent target of hydroxamate drugs. This ill-annotated palmitoyl-CoA hydrolase is inhibited by 24 HDAC inhibitors at low nM potency. Both enzymatic inhibition and knocking down the protein led to the accumulation of extracellular vesicles. Given the importance of exosome biology in neurological diseases or cancer, this HDAC-independent drug effect creates the incentive for considering MBLAC2 as a target for drug discovery.
Analysis of Axillary Management Following Mastectomy With a Positive Sentinel Lymph Node Biopsy
Authors: James Ted A. , Pardo Jaime A. , Fan Betty , Mele Alessandra , Valero Monica , Recht Abram , Davis Roger B. 
Published: July 2021

Abstract
BACKGROUND: Axillary lymph node dissection (ALND) with or without postmastectomy radiation therapy (PMRT) was traditionally the standard of care for patients with a positive sentinel node following mastectomy. However, recent clinical trial data has led to an interest in de-escalating therapy and a debate over optimal axillary management. We sought to assess current practice patterns and the impact of different approaches to managing positive sentinel nodes following mastectomy. METHODS: Using the National Cancer Database (NCDB), patients with clinical T1-2 N0 M0 breast cancer from 2012-2015 treated with a mastectomy who were found to have a single positive sentinel node were analyzed. A logistic regression model stratified by patients’ characteristics in association to the type of axillary treatment received was performed.RESULTS: We identified 12,137 women with a positive sentinel lymph node biopsy (SLNB) at the time of mastectomy. Of these, 4,221 had an ALND; 1,609 received PMRT; 1,565 underwent combination therapy, and 4,742 had no further treatment following SLNB (NFT). Factors associated with an increased likelihood of further axillary treatment included younger age (<40), Midwest location, larger primary tumor size (T2), and high grade. There was no difference in short-term overall survival among these approaches. CONCLUSION: Our study indicates significant practice variation in the axillary management of patients with metastasis limited to a single sentinel node undergoing mastectomy. The clinical variation observed raises the possibility of unnecessary or overtreatment of the axilla. These findings suggest a need to expand the adoption of evidenced-based clinical protocols to improve quality of care.
A Low Complexity Adaptive Scheme for Massive MIMO Systems with MRC Receiver
Authors: Radbord Amin 
Published: July 2021

Abstract
This paper evaluates a low-complexity Multiuser Adaptive Modulation (MAM) scheme for an uplink massive multiple input and multiple output (MIMO) systems with maximum-ratio combining linear receivers, which requires only slow-varying large-scale shadowing information for the users. For a channel that includes both small-scale and large-scale fading, the probability density function of signal-to-interference-plus-noise ratio is analytically calculated for the first time and verified by simulation. Also, closed-form expressions are derived for the average spectral efficiency and bit error outage. Since, the MRC is desirable in low transmit power, the previous expression is approximated at low power values. Moreover, the paper investigates different users’ arrangements around the base station to achieve the maximum possible bit rate. For MAM with joint power control, optimal power adaptation strategy switching thresholds are also acquired. Compared to the research literature, the proposed adaptive scheme by the MRC receiver, gives similar performance as a proper adaptive method at low power transmission.
Research And Simulation of The Sheet Leveling Machine Manufacturing Capabilities
Authors: Gribkov Eduard P. , Kovalenko Andrii K. , Hurkovskaya Svetlana S. 
Published: July 2021

Abstract
One of the main tasks to determine the technological settings of the sheet leveling machine (leveler) is to identify the maximum roll overlap. In the well-known works on the study of sheet metal-roll leveling, the overlaps are set on the basis of experimental data, that is applicable for the leveling sheets from material with the 800 MPa yield strength. But for materials with a higher yield strength additional research is required. The purpose of this work is to establish criteria affecting the maximum allowable overlap for the working rolls by the leveler and to determine the dependencies for the most rational working rolls adjustment. When solving the set tasks, developed by the authors, a numerical analytical mathematical model of the leveling process was used as an objective function, that allows to determine the energy-power characteristics and the sheet curvature after leveling, depending on the individual settings of the leveler rolls. Within the framework of studies, an algorithm of a mathematical model was developed that allows to determine the leveler technological settings necessary for correcting the longitudinal curvature and comes down to determining the aligning coordinates for each of the movable rolls depending on the known characteristics of the metal being processed, the leveler geometric parameters, and the permissible value of the sheet residual curvature after leveling. In consequence of studying the influence of the working rolls setup on the sheet metal-roll quality during leveling on a multi-roll leveling machine, the laws for the rational rolls positioning were established. The linear and sinusoidal laws were identified as optimal ones. Boundary factors were also established as affecting the maximum overlap of the rolls. These factors included the condition of the pickup and the condition of the rolls strength. Using the example of implementing the developed finite element model of the sheet leveling process at the leveler of NKMZ (Ukraine), it was found that the pickup condition has a dominant influence when leveling sheets with a thickness of less than 6 mm, and with larger thicknesses the condition of the rolls strength is prevailing. With a decrease in the yield strength of the material or the width of the sheets, this ratio will be redistributed towards the pickup condition.