bluuebunny/crossref_metadata_2025_split huggingface dataset:
Irritability and Inattention Not Sad Low Mood Predict Impulsiveness in Children and Adolescents With Major Depressive Disorder and Persistent Depressive Disorder
Authors: Vance Alasdair , Winther Jo 
Published: May 2021

Abstract
The specific relationships between impulsiveness, inattention, sad, low mood, and irritability have not been systematically examined in young people with major depressive disorder with and without persistent depressive disorder. The relationships are important to clarify because these symptom dimensions may increase suicidal risk in children and adolescents with these depressive disorders. A total of 313 medication-naive young people (aged 6–16 years) with active major depressive disorder (MDD) alone, persistent depressive disorder (DD) alone, and comorbid MDD and DD were identified. “Inattention,” “sad/unhappy,” and “irritable” mood were identified by parent standardized questionnaire. Standard multiple regression was used to investigate how well inattention, sad/unhappy, and irritable mood predict impulsiveness. Inattention (32% of the variance, increased) and irritable mood (5% of the variance, increased) both made independent significant contributions to impulsiveness, whereas sad/unhappy mood did not. Decreasing irritability via more targeted and comprehensive management approaches may ameliorate impulsiveness in young people with these depressive disorders.
Quantitative susceptibility mapping captures chronic multiple sclerosis rim lesions with greater myelin damage: Comparison with high-pass filtered phase MRI
Authors: Huang Weiyuan , Sweeney Elizabeth M. , Kaunzner Ulrike W. , Wang Yi , Gauthier Susan A. , Nguyen Thanh D. 
Published: May 2021

ABSTRACTBackgroundChronic active MS lesions with paramagnetic rim can be identified by high-pass filtered (HPF) phase imaging or quantitative susceptibility mapping (QSM).PurposeThe objective was to compare the ability of HPF and QSM to identify MS lesions with greater myelin damage and to distinguish MS patients with increased clinical disability.Material and MethodsEighty-six patients were scanned with gradient echo sequence for lesion rim detection and FAST-T2 sequence for myelin water fraction (MWF) mapping. Chronic lesions were classified based on the presence/absence of rim on HPF and QSM images (HPF rim+/QSM rim+, HPF rim+/QSM rim-, HPF rim-/QSM rim+, HPF rim-/QSM rim-). A lesion-level linear mixed-effects model with MWF as outcome was used to compare myelin damage among the lesion groups. A multivariate patient-level linear regression model was fit to establish the association between Expanded Disease Status Scale (EDSS) and the number of rim lesions (zero vs. one or more).ResultsOf 2229 lesions, 96 (8.8%) were HPF rim+/QSM rim+, 211 (9.5%) were HPF rim+/QSM rim-, and the remainder had no rim. Adjusting for other factors, HPF rim+/QSM rim+ lesions had on average significantly lower MWF than both HPF rim+/QSM rim-(p<0.001) and HPF rim-/QSM rim-(p<0.001) lesions, while the MWF difference between HPF rim+/QSM rim- and HPF rim-/QSM rim-lesions was not statistically significant (p=0.309). Having at least one QSM rim+ lesion was associated with an increase in EDSS compared to having no QSM rim+ lesions, holding all other factors constant (p=0.026). The relationship between having one or more HPF rim+ lesions vs. having no HPF rim+ lesions and EDSS was not statistically significant.ConclusionQSM identifies chronic MS lesions with paramagnetic rim that on average have greater myelin damage. QSM may be a valuable tool for studying the impact of rim lesions on clinical disability in MS.
Alternative splicing regulates the physiological adaptation of the mouse hind limb postural and phasic muscles to microgravity
Authors: Henrich Mason , Ha Pin , Adams John S. , Soo Chia , Ting Kang , Stodieck Louis , Chun Rene 
Published: May 2021

AbstractMuscle atrophy and fiber type alterations are well-characterized physiological adaptations to microgravity with both understood to be primarily regulated by differential gene expression (DGE). While microgravity-induced DGE has been extensively investigated, adaptations to microgravity due to alternative splicing (AS) have not been studied in a mammalian model. We sought to comprehensively elucidate the transcriptomic underpinnings of microgravity-induced muscle phenotypes in mice by evaluating both DGE and changes in AS due to extended spaceflight. Tissue sections and total RNA were isolated from the gastrocnemius and quadriceps, postural and phasic muscles of the hind limb, respectively, of 32-week-old female BALB/c mice exposed to microgravity or ground control conditions for nine weeks. Immunohistochemistry disclosed muscle type-specific physiological adaptations to microgravity that included i) a pronounced reduction in muscle fiber cross-sectional area in both muscles and ii) a prominent slow-to-fast fiber type transition in the gastrocnemius. RNA sequencing revealed that DGE and AS varied across postural and phasic muscle types with preferential employment of DGE in the gastrocnemius and AS in the quadriceps. Gene ontology analysis indicated that DGE and AS regulate distinct molecular processes. Various non-differentially expressed transcripts encoding musculoskeletal proteins (Tnnt3, Tnnt1, Neb, Ryr1,andTtn) and muscle-specific RNA binding splicing regulators (Mbnl1 and Rbfox1) were found to have significant changes in AS that altered critical functional domains of their protein products. In striking contrast, microgravity-induced differentially expressed genes were associated with lipid metabolism and mitochondrial function. Our work serves as the first comprehensive investigation of coordinate changes in DGE and AS in large limb muscles across spaceflight. We propose that substantial remodeling of pre-mRNA by AS is a major component of transcriptomic adaptation of skeletal muscle to microgravity. The alternatively spliced genes identified here could be targeted by small molecule splicing regulator therapies to address microgravity-induced changes in muscle during spaceflight.
Maximizing genetic representation in seed collections from populations of self and cross-pollinated banana wild relatives
Authors: Kallow Simon , Panis Bart , Vu Dang Toan , Vu Dang Tuong , Paofa Janet , Mertens Arne , Swennen Rony , Janssens Steven B. 
Published: May 2021

AbstractBackgroundConservation of plant genetic resources, including the wild relatives of crops, plays an important and well recognised role in addressing some of the key challenges faced by humanity and the planet including ending hunger and biodiversity loss. However, the genetic diversity and representativeness ofex situcollections, especially that contained in seed collections, is often unknown. This limits meaningful assessments against conservation targets, impairs targeting of future collecting and limits their use.We assessed genetic representation of seed collections compared to source populations for three wild relatives of bananas and plantains. Focal species and sampling regions wereMusa acuminatasubsp.banksii(Papua New Guinea),M. balbisiana(Viet Nam) andM. maclayi s.l.(Bougainville, Papua New Guinea). We sequenced 445 samples using suites of 16-20 existing and newly developed taxon-specific polymorphic microsatellite markers. Samples of each species were from five populations in a region; 15 leaf samples and 16 seed samples from one infructescence (‘bunch’) for each population.ResultsAllelic richness of seeds compared to populations was 51%, 81% and 93% (M. acuminata, M. balbisianaandM. maclayirespectively). Seed samples represented all common alleles in populations but omitted some rarer alleles. The number of collections required to achieve the 70% target of the Global Strategy for Plant Conservation was species dependent, relating to mating systems.Musa acuminatapopulations had low heterozygosity and diversity, indicating self-fertilization; many bunches were needed (>15) to represent regional alleles to 70%; over 90% of the alleles from a bunch are included in only two seeds.Musa maclayiwas characteristically cross-fertilizing; only three bunches were needed to represent regional alleles; within a bunch, 16 seeds represent alleles.Musa balbisiana,considered cross-fertilized, had low genetic diversity; seeds of four bunches are needed to represent regional alleles; only two seeds represent alleles in a bunch.ConclusionsWe demonstrate empirical measurement of representation of genetic material in seeds collections inex situconservation towards conservation targets. Species mating systems profoundly affected genetic representation in seed collections and therefore should be a primary consideration to maximize genetic representation. Results are applicable to sampling strategies for other wild species.
A quantitative review of abundance-based species distribution models
Authors: Waldock Conor , Stuart-Smith Rick D. , Albouy Camille , Cheung William W. L. , Edgar Graham J. , Mouillot David , Tjiputra Jerry , Pellissier Loïc 
Published: May 2021

AbstractThe contributions of species to ecosystem functions or services depend not only on their presence in a given community, but also on their local abundance. Progress in predictive spatial modelling has largely focused on species occurrence, rather than abundance. As such, limited guidance exists on the most reliable methods to explain and predict spatial variation in abundance. We analysed the performance of 68 abundance-based species distribution models fitted to 800,000 standardised abundance records for more than 800 terrestrial bird and reef fish species. We found high heterogeneity in performance of abundance-based models. While many models performed poorly, a subset of models consistently reconstructed range-wide abundance patterns. The best predictions were obtained using random forests for frequently encountered and abundant species, and for predictions within the same environmental domain as model calibration. Extending predictions of species abundance outside of the environmental conditions used in model training generated poor predictions. Thus, interpolation of abundances between observations can help improve understanding of spatial abundance patterns, but extrapolated predictions of abundance, e.g. under climate change, have a much greater uncertainty. Our synthesis provides a roadmap for modelling abundance patterns, a key property of species’ distributions that underpins theoretical and applied questions in ecology and conservation.
Increased biosynthesis of acetyl-CoA in the yeast Saccharomyces cerevisiae by overexpression of a deregulated pantothenate kinase gene
Authors: Olzhausen Judith , Grigat Mathias , Seifert Larissa , Ulbricht Tom , Schüller Hans-Joachim 
Published: May 2021

AbstractCoenzyme A (CoA) and its derivatives such as acetyl-CoA are essential metabolites for several biosynthetic reactions. In the yeast S. cerevisiae, five enzymes (encoded by essential genes CAB1-CAB5; coenzyme Abiosynthesis) are required to perform CoA biosynthesis from pantothenate, cysteine and ATP. Similar to enzymes from other eukaryotes, yeast pantothenate kinase (PanK, encoded by CAB1) turned out to be inhibited by acetyl-CoA. By genetic selection of intragenic suppressors of a temperature-sensitive cab1 mutant combined with rationale mutagenesis of the presumed acetyl-CoA binding site within PanK, we were able to identify the variant CAB1 W331R, encoding a hyperactive PanK completely insensitive to inhibition by acetyl-CoA. Using a versatile gene integration cassette containing the TPI1 promoter, we constructed strains overexpressing CAB1 W331R in combination with additional genes of CoA biosynthesis (CAB2, CAB3, HAL3, CAB4 and CAB5). In these strains, the level of CoA nucleotides was 15-fold increased, compared to a reference strain without additional CAB genes. Overexpression of wild-type CAB1 instead of CAB1 W331R turned out as substantially less effective (4-fold increase of CoA nucleotides). Supplementation of overproducing strains with additional pantothenate could further elevate the level of CoA (2.3-fold). Minor increases were observed after overexpression of FEN2 (encoding a pantothenate permease) and deletion of PCD1 (CoA-specific phosphatase). We conclude that the strategy described in this work may improve the efficiency of biotechnological applications depending on acetyl-CoA.Key pointsA gene encoding a hyperactive yeast pantothenate kinase (PanK) was constructed.Overexpression of CoA biosynthetic genes elevated CoA nucleotides 15-fold.Supplementation with pantothenate further increased the level of CoA nucleotides.
A Neuroscience-Inspired Spiking Neural Network for Auditory Spatial Attention Detection Using Single-Trial EEG
Authors: Faghihi Faramarz , Cai Siqi , A.Moustafa Ahmed 
Published: May 2021

AbstractRecently, studies have shown that the alpha band (8-13 Hz) EEG signals enable the decoding of auditory spatial attention. However, deep learning methods typically requires a large amount of training data. Inspired by “sparse coding” in cortical neurons, we propose a spiking neural network model for auditory spatial attention detection. The model is composed of three neural layers, two of them are spiking neurons. We formulate a new learning rule that is based on firing rate of pre-synaptic and post-synaptic neurons in the first layer and the second layer of spiking neurons. The third layer consists of 10 spiking neurons that the pattern of their firing rate after training is used in test phase of the method. The proposed method extracts the patterns of recorded EEG of leftward and rightward attention, independently, and uses them to train network to detect the auditory spatial attention. In addition, a computational approach is presented to find the best single-trial EEG data as training samples of leftward and rightward attention EEG. In this model, the role of using low connectivity rate of the layers and specific range of learning parameters in sparse coding is studied. Importantly, unlike most prior model, our method requires 10% of EEG data as training data and has shown 90% accuracy in average. This study suggests new insights into the role of sparse coding in both biological networks and brain-inspired machine learning.
The zebrafish meiotic cohesion complex protein Smc1b is required for key events in meiotic prophase I
Authors: Islam Kazi Nazrul , Modi Maitri Mitesh , Siegfried Kellee Renee 
Published: May 2021

ABSTRACTThe eukaryotic structural maintenance of chromosomes (SMC) proteins are involved in key processes of chromosome structure and dynamics. SMC1β was identified as a component of the meiotic cohesion complex in vertebrates, which aids in keeping sister chromatids together prior to segregation in meiosis II and is involved in association of homologous chromosomes in meiosis I. The role of SMC1β in meiosis has primarily been studied in mice, where mutant male and female mice are infertile due to germ cell arrest at pachytene and metaphase II stages, respectively. Here, we investigate the function of zebrafish Smc1b to understand the role of this protein more broadly in vertebrates. We found that zebrafish smc1b is necessary for fertility and has important roles in meiosis, yet has no other apparent roles in development. Therefore, smc1b functions primarily in meiosis in both fish and mammals. In zebrafish, we showed that smc1b mutant spermatocytes initiated telomere clustering in leptotene, but failed to complete this process and progress into zygotene. Furthermore, mutant spermatocytes displayed a complete failure of homolog pairing and synapsis. Interestingly, meiotic DNA double strand breaks occurred in the absence of Smc1b despite failed pairing and synapsis. Overall, our findings point to an essential role of Smc1b in the leptotene to zygotene transition during zebrafish spermatogenesis. In addition, ovarian follicles failed to form in smc1b mutants, suggesting an essential role in female meiosis as well. Our results indicate that there are some key differences in Smc1b requirement in meiosis among vertebrates: while Smc1b is not required for homologue pairing and synapsis in mice, it is essential for these processes in zebrafish.
Evaluation of podocyte Rac-1 induced kidney disease by modulation of TRPC5
Authors: Polat Onur K. , Isaeva Elena , Zhu Ke , Noben Manuel , Sudhini Yashwanth , Samelko Beata , Kumar Varsha S. , Wei Changli , Altintas Mehmet M. , Dryer Stuart E. , Sever Sanja , Staruschenko Alexander , Reiser Jochen 
Published: May 2021

AbstractBackgroundTransient receptor potential channel 5 (TRPC5) is a non-selective cationic ion channel expressed in brain, kidney and other organs where its activation underlies podocyte injury in chronic kidney diseases. Specifically, it has been suggested that a podocyte TRPC5 plasma membrane relocation and channel activation following injury results from activation of Rac-1, propagating podocyte dysfunction and proteinuria. However, previous TRPC5 transgenic mouse studies had questioned a pathogenic role for TRPC5 in podocytes. This investigation was designed to specifically evaluate podocyte Rac-1 activation in the context of functional TRPC5 or a TRPC5 pore mutant to assess effects on proteinuria.Materials and MethodsWe employed single cell patch-clamp studies of cultured podocytes and studied proteinuria in transgenic mouse models to characterize the effects of TRPC5 following podocyte Rac-1 activation.ResultsInhibition of TRPC5 by small molecules reportedly ameliorated proteinuria in murine models of proteinuric kidney diseases. In order to directly examine TRPC5 function following Rac-1-induced podocyte injury, we analyzed TRPC5 inhibition in podocyte specific Rac-1 (active) transgenic mice. In addition, we generated a double-transgenic mouse constitutively overexpressing either TRPC5 (TRPC5WT) or a TRPC5 dominant-negative pore mutant (TRPC5DN) in concert with podocyte specific and inducible activation of active Rac-1 (Rac-1Dtg). In electrophysiological experiments, active TRPC5 was detected in primary podocytes overexpressing TRPC5 but not in podocytes with endogenous TRPC5 expression, nor with Rac-1 overexpressing podocytes. TRPC5 inhibition did not change proteinuria in mice with active podocyte Rac-1, nor did an increase or loss of TRPC5 activity affected podocyte injury in Rac-1Dtg animals. Administration of TRPC5 inhibitors, ML204 and AC1903, did not alleviate podocyte Rac-1 induced proteinuria.ConclusionTRPC5 inhibition did not modify podocyte Rac-1 induced proteinuria in mice.Significance StatementTRPC5 is a calcium conducting ion channel involved in a plethora of biological functions in the brain, kidney and other organs. In proteinuric kidney diseases, others proposed a model that links activation of small GTPase Rac-1 in podocytes to activation of TRPC5 channels propagating cellular injury and eventually leading to progressive kidney disease. To test this hypothesis, we have developed a novel transgenic mouse model that employs podocyte Rac-1 activation in the presence or absence of a functional TRPC5 channel. Our data shows that transgenic mice with activated Rac-1 in podocytes did not enhance endogenous TRPC5 expression or its activity. Furthermore, TRPC5 blockade or activation did not modify Rac-1 induced proteinuria in mice.
Aggressive bidding strategies in real estate auctions – a structural equation modelling (SEM) approach
Authors: Dalland Simen , Hammervold Randi , Karlsen Henrik Tangen , Oust Are , Sønstebø Ole Jakob 
Published: May 2021

Purpose
This paper aims to study aggressive bidding strategies in real estate auctions – a structural equation modelling (SEM) approach.

Design/methodology/approach
The authors use two data sets to study aggressive bidding strategies. First, the results from a survey with 1,803 participants examining real estate auctions are used to identify bidding strategies and related motivations. Second, the authors apply SEM by using data from 1,078 exclusive auction journals from real estate sales in Norway to study both the direct and indirect price effects of the bidding strategies.

Findings
The authors define four aggressive bidding strategies: high opening bid, high bid increase (jump bids), short acceptance deadline and short response time. The authors find that all four strategies yield a higher sales price. Bidders can actively influence the behaviour of the other participants and cool the potential auction fever, thus reducing the final price premium.

Originality/value
This paper gives households, investors and policymakers a better understanding of how bidding strategies affect real estate auctions and the final price.
Travelers’ motivations to adopt augmented reality (AR) applications in a tourism destination
Authors: Shin Hyejo Hailey , Jeong Miyoung 
Published: May 2021

PurposeThis study aims to identify travelers’ motivational factors to adopt augmented reality (AR) applications at tourism destinations. Furthermore, this study seeks to investigate the role of personal innovativeness as a moderator in the relationships between motivations and travelers’ behavioral outcomes.Design/methodology/approachBuilding upon two theories, cognitive evaluation theory and self-presentation theory, this study developed a conceptual framework to identify travelers’ inclination to use AR applications at tourism destinations. A sample size of 473 was collected through an online self-administered survey. Using partial least square (PLS) analysis, the proposed model was examined. To examine the moderating effect of personal innovativeness, a multi-group analysis was conducted with two groups: high-innovative and low-innovative. PLS method is used to test the study’s theoretical model.FindingsFindings of this study indicate that both hedonic and utilitarian motivations significantly influenced travelers’ attitudes toward AR applications at tourism destinations. Self-presentation motivation appears to have a significantly positive impact on travelers’ attitudes toward AR applications only for the highly innovative group.Originality/valueThis study helps a better understanding of what motivates travelers to adopt AR applications at tourism destinations by integrating preeminent theories and applying them to the tourism context.
Exposure to green space and prevention of anxiety and depression among young people in urban settings: a global scoping review
Authors: Reece Rebecca , Bray Isabelle , Sinnett Danielle , Hayward Robert , Martin Faith 
Published: May 2021

Purpose
There is a mental health crisis, particularly among young people. Despite many young people living in urban settings, reviews about the association between exposure to green or natural environments and mental health tend to focus on either children or adults. The aim of this review is to examine the scope of the global literature for this age group, to inform a systematic review on the role of exposure to green space in preventing anxiety and depression amongst young people aged 14–24 years.

Design/methodology/approach
Seven databases were searched for quantitative and qualitative sources published from January 2000 to June 2020. This identified 201 sources and their characteristics are described here. Gaps in the literature are also highlighted.

Findings
The number of relevant studies published per year has increased over time. Most studies are set in North America (28%) or Europe (39%). The most common study designs were observational (34%) or experimental (28%). A wide range of exposures and interventions are described.

Research limitations/implications
This review included literature from predominantly high-income countries and has shown the under-representation of low-middle income countries and lack of ethnic diversity in study populations. It has also highlighted the lack of clinical measures of anxiety and depression as outcomes.

Originality/value
This inter-disciplinary review has contributed to the field by describing the geographic distribution of the literature and the broad range of exposures to green spaces being reported. Unlike previous scoping reviews, this review focused specifically on young people and on measures of anxiety and depression and their pre-cursers.