bluuebunny/crossref_metadata_2025_split huggingface dataset:
Role of the CtrA cell cycle regulator in the bloodborne bacterial pathogen, Bartonella quintana.
Authors:   , Thomson Robert Haydn 
Published: December 2021

<p>Bartonella quintana is an important re-emerging human pathogen and the causative agent of trench fever. It utilizes a stealth invasion strategy to infect hosts and is transmitted by lice. Throughout infection it is crucial for the bacteria to maintain a tight regulation of cell division, to prevent immune detection and allow for transmission to new hosts. CtrA is an essential master cell cycle regulatory protein found in the alpha-proteobacteria. It regulates many genes, ensuring the appropriate timing of gene expression and DNA replication. In the model organism Caulobacter crescentus, it regulates 26% of cell cycle-regulated genes. CtrA has been reported to bind two specific DNA motifs in gene promoter regions, TTAAN7TTAAC, and TTAACCAT. Genes regulated by CtrA encode proteins with a wide range of activities, including initiation of DNA replication, cell division, DNA methylation, polar morphogenesis, flagellar biosynthesis, and cell wall metabolism. However, the role of the CtrA homologue in Bartonella spp. has not been investigated. In this project we aimed to make an initial characterisation of the master cell cycle regulator CtrA. This was done by identifying gene regulatory regions containing putative CtrA binding sites and testing for direct interactions via a -galactosidase assay. It was found B. quintana CtrA shared 81 % amino acid identity with its C. crescentus homologue. Within the genome of B. quintana str. Toulouse we discovered 21 genes containing putative CtrA binding sites in their regulatory regions. Of these genes we demonstrated interactions between CtrA and the promoter region of ftsE a cell division gene [1], hemS, and hbpC, two heme regulatory genes. We also found no evidence of CtrA regulating its own expression, which was unexpected because CtrA autoregulation has been demonstrated in C. crescentus.</p>
Autonomy of Public Service Delivery Units in Vietnam: An Institutional Perspective
Authors:   , Vo Minh Thi Hai 
Published: December 2021

<p>Intrigued by the burgeoning commercialisation of public services in Vietnam, this research seeks to understand the nature of autonomy that public service delivery units have been given as part of the greater effort of the Government of Vietnam to separate business and service provision from state management. In addressing this objective, the research bases its analysis on historical institutionalism and sociological institutionalism that illuminate insights into institutional factors shaping the autonomy policies and the implementation of the policies in practice. The main data sources the research draws on are legal documents, government reports and in-depth interviews with managers and staff of public service delivery units. The research findings illustrate that autonomy is very limited in many aspects of management because the autonomy reforms are largely influenced by the socialist-oriented market path dependence. Amidst the exhaustion of resources and legitimacy due to the regime’s flawed economic structure and inherent problems of cronyism and corruption, autonomy is utilized primarily as a strategic instrument to mobilize resources indispensable for the socialist survival. This policy direction creates incentives for the various forms of rent-seeking and corrupt behaviour among public service delivery units, occasioning the rapid and aggressive commercialization of public services. While the root of these problems lies in the intent of the policies, these problems are further nurtured by historical and cultural factors, and seriously compounded by the absence of an effective regulatory and accountability system and the lack of political commitment to fight corruption from the top leaders. The research addresses the gap in the academic literature by offering an illuminating insight into the nature of autonomy of a country context and demonstrating the merit of converging historical institutionalism and sociological institutionalism for the study of public policies. The research’s significant contribution to policy-making is that it highlights the need to build institutions to fit in well with the reform ideas, especially when such ideas are borrowed or transferred from advanced countries with well-developed institutional foundations.</p>
The South Pacific Convergence Zone as Depicted in Reanalysis and Satellite Products
Authors:   , Luke Harvey Thomas 
Published: December 2021

<p>The South Pacific Convergence Zone (SPCZ) is the largest rainfall feature in the Southern Hemisphere, and is a critical component of the climate of Southwest Pacific Island nations. The small size and isolated nature of these islands leaves them vulnerable to short and long term changes in the position of the SPCZ. Its location and strength is strongly modulated by the El Niño-Southern Oscillation (ENSO) cycle and the Inter-decadal Pacific Oscillation (IPO), leading to large inter-annual and decadal variability in rainfall across the Southwest Pacific. Much of the analysis on the SPCZ has been restricted to the modern period, more specifically the “satellite era”, starting in 1979. Here, the representation of the SPCZ in the Twentieth Century Reanalysis (20CR) product, which reconstructs the three-dimensional state of the atmosphere based only on surface observations is discussed. The performance of two versions of the 20CR (versions 2 and 2c) in the satellite era is tested via inter-comparison with other reanalysis and observational satellite products, before using 20CR version 2c (20CRv2c) to perform extended analysis back to the early twentieth century. This study demonstrates that 20CR performs well in the satellite era, and is considered suitable for extended analysis. It is established that extra data added in the SPCZ region between 20CR versions 2 and 2c has improved the representation of the SPCZ during 1908-1958. Well-established relationships between ENSO and the IPO with the SPCZ are shown to be present through the entire 1908-2011 period, although it is suggested that the physical link between the IPO and the SPCZ has changed between the first and second half of the twentieth century. Finally, evidence of a southward trend of the SPCZ over the past century is presented, potentially due to an expansion of the tropics as a result of climate change.</p>
Dead Among the Living: Burning Bodies in Inner City Wellington
Authors:   , Moller Zoe Jaye 
Published: December 2021

<p>The architecture of cremation has struggled to embrace an identity; it has remained ambiguous in its architectural typology and religious association since it was first introduced into western society. Additionally, the absence of a ritual place for death in urban life is one manifestation of the contemporary idea that death does not belong in the modern living city. Death is seen as having no place in a society obsessed with youth and vigour; it has become an architectural taboo. The increased reluctance to physically address death as the inevitable consequence to life has resulted in death associated architecture eroding to the point where it has become absent in our everyday lives. With the expansion of Wellington during the 1800’s, cemeteries formerly on the outskirts (Mount and Bolton Streets) became engulfed by the sprawling city. Overflowing with corpses by the 1900’s, these sites now remain dormant, eliminating any opportunity for the public to ‘see’ death daily. Situating a crematorium within a Wellington urban context will not only address this issue, but also successfully meet the demand for more burial spaces, as Makara Cemetery is nearing capacity, and Karori Cemetery is already full. A site located in the ‘dead centre’ of Wellington’s central business district becomes the testing ground for a new urban crematorium – one that aims to reduce the anxiety around death by inclusion of it within people’s everyday lives. It aims to provide mourners with a more meaningful experience, and the general public a cosmopolitan necropolis. The presence of an urban crematorium and columbarium provides continual opportunities for people to reflect on their own mortality, honour and remember the dead, and be reminded to live while they can. A methodological approach of testing architectural sequences in relation to pattern language theory will allow for a thematic progression for mourners from sorrow to acceptance through the use of light, shadow, and sectional arrangements. This investigation into the meaningfulness of relationships between people and buildings, life and death, translates into spaces ready to be further invested with meaning by mourners.</p>
The Hyphenated Zone: Exploring the Connection Between Actual and Virtual Space
Authors:   , Gill Gabrielle 
Published: December 2021

<p>This research operates at the nexus between actual and virtual space. In interior architecture we can use tools to produce virtual experience, being immersed in a world or space different to your physical location. These tools can include, but are not limited to, actual materials, forms, spaces, and arrangements. Virtual space is described as the non-material spatial experience. This experience disconnects us from the physical actual environment that we live in. The best example of this is that of a cinematic experience. When we watch a film in a cinema, the actual physical environment we usually occupy is re-contextualised through a lack of light; our focus is then given to the light and movement produced by the projected image where we become encapsulated by this sense of virtual that we cannot control. We experience a sense of space different to our own and occupy this space although it remains less tangible than the physical world we live in. This thesis proposes a redesign to the public areas for the site of — Ngā Taonga Sound and Vision in Wellington. Currently, the only access the public has to the collection is through digital means, a small cinema and collection of computers in the media library. This project aims to create engaging, embodied encounters with the digital collection of Ngā Taonga Sound and Vision through a series of interior installations within the site. These installations extend the virtual interior created by the sound and moving images further into the boundaries of the actual, physical interior. The exhibition spaces of Ngā Taonga Sound and Vision offer a rich testing site, and provokes the question, how can we create more immersive, engaging encounters of the incredible films collected by Ngā Taonga? The purpose of an archive is to preserve the past for future generations. Although the site currently allows some opportunities for the public to access the collection, it lacks a sense of immersion that can be explored through interior architecture interventions.</p>
Learning to be Mindful: An Exploratory Study into the Perceived Benefits of Mindfulness-Based Stress Reduction Training for a Music Therapy Student
Authors:   , Graham Jessica O. M. 
Published: December 2021

<p>Mindfulness training has become very popular in recent years and has proven successful for reducing anxiety and depression and enhancing coping skills (amongst other benefits). This research project explores the perceived benefits of mindfulness-based stress reduction (MBSR) training on a music therapy student working with young people with complex needs in special education. Secondary analysis of clinical records (session notes and a reflexive journal) was employed and included the use of both inductive and deductive methods of analysis. Five themes were developed (framed as themes of learning) including: Presence, Non-striving, Beginner’s Mind, Acceptance, and Patience with a final key finding being the effect of modelling to the students. Findings showed these benefits emerging through a journey from early data (before MBSR training) to later data (after MBSR training) and suggest that mindfulness training can be beneficial for both music therapy students and (indirectly) to those they work with. The themes and perceived benefits proved very interconnected with each relating to several others and ‘Being Present’ emerging as an over-arching theme. Similarities between benefits discovered and certain principles of music therapy were discussed and whether the benefits found may have occurred naturally through the course of music therapy training amongst other factors of change and natural growth.</p>
Intangible Narratives; An investigation on immersive architectural visualisation in VR
Authors:   , Leung Kar Wing 
Published: December 2021

<p>Within the current context of digital architectural construction and immersive representation exists a problem of ‘ocularcentrism’ as defined by Pallasmaa. Recent immersive developments such as Google Earth VR, or Realities.io, focus primarily in working within the tangible experience, purely emphasising on geometric forms and physical artefacts. They largely ignore the more experiential and intangible qualities of space, which are crucial elements in a real embodied physical experience of architecture. Phenomenologists such as Pallasmaa, Holl, and Zumthor expand on this, identifying that architecture clearly exists as something beyond just the tangible. Architecture fundamentally is an experiential and spatial art, incorporating not just the geometric sense of space but also aspects of the intangible such as sound, movement, lighting and interaction. As a result of this tangible-bias, virtual-reality’s current use within architecture as an immersive medium is largely limited. Most developments can be described as ‘an immersive virtual photograph’ of architecture rather than an experience of one. This research is a response to studies that directly address the problem of ‘ocularcentrism’ while also exploring the methods and techniques that can translate intangible qualities into virtual architecture. Utilising a Real-Time Virtual Environment (RTVE) and Virtual Reality (VR) tool set, this paper virtually reconstructs the case study of the Kowloon Walled City. It proposes a novel working methodology for immersive architectural visualisation with VR and adds to the on-going research in the field of phenomenology within architecture.</p>
Re: Privacy; investigating the accessibility of online legal documents in relation to the privacy paradox
Authors:   , Calvert Elizabeth Ivy 
Published: December 2021

<p>Website Terms of Service and Privacy Policy documents are the delivery methods employed by online services to inform internet users of how information is treated on their platform. When users fail to read or understand these documents, many issues can arise. These issues take shape in the form of unwillingness to use services, misunderstandings of how online data is treated, and/or user concerns for personal privacy. Currently, the leading factors influencing user motivation to read these documents include (but are not limited to) document length, complicated language, and time required to read. To encourage users to engage with these documents, this research investigates the delivery methods service providers use to present these legal documents online. During the course of this investigation, the proposal of a new method of presenting website Privacy Policies to users is explored. This tool, ‘Re: Privacy’, was developed with the aim of increasing user awareness of online data treatment, whilst minimising the factors that dissuade users from reading the official document. Published alongside Re: Privacy is a comprehensive analysis of website legal documents and user understanding of these documents. This analysis also provides an investigation into user understanding of current website legal documents, and how user understanding can be improved.</p>
Exploring the Impact of Resettlement on Women: A Case Study of the Nam Mang 3 Hydropower Dam in Central Laos
Authors:   , Lueyeevang Singkham 
Published: December 2021

<p>Over the last decade, construction of hydropower dams has increased rapidly around the world, including in developing countries. For many countries including Laos, energy production and export play a significant role in promoting and boosting economic growth and development. Energy production generates substantial revenue and foreign exchange from exporting electricity and expands economic activity domestically. However, construction of hydropower dams also causes negative effects on the people who live at and around the dam site. Some local communities have been affected indirectly, while others require relocation to other areas bringing significant change, including for women. This research explores the impact of resettlement from hydropower dam creation on women by using the Nam Mang 3 hydropower dam in central Laos as a case study. This dam, completed in 2005, required the relocation of approximately 150 households from two villages at the dam site. The research draws on a social constructivist epistemology, qualitative methods including semi-structured interviews, and analysis of relevant policy documents. Interviews involved 18 participants including both male and female from the three affected villages. Results indicate that the resettlement of villagers by the Nam Mang 3 hydropower dam has generally improved living conditions of the resettled communities. Women were found to have greater opportunities to benefit from home-based business, employment and wage labouring. In addition, with better access to modern facilities and services, women have been able to save greater time from agricultural activities and divert this time saving to other economic activities. Overall, access to water supplies, roads, and transport have reduced women’s workload significantly. Access to improved health services and facilities have also improved women’s wellbeing. Girls have greater opportunities to attend school and seek employment in towns. However, this research identified some challenges experienced by the resettled such as the reduction of agricultural and grazing land area, and that new livelihood options were not accessible to all women. Instead of these challenges, the experience with the Nam Mang 3 hydropower project has highlighted one of the key lessons learnt that is worth highlighting for future resettlement programs, which is to have the resettlers fully engaged in the entire process of resettlement and livelihood strategy development.</p>
Freeform 3D Printing: A Sustainable, Efficient Construction Alternative
Authors:   , Papageorge Armano 
Published: December 2021

<p>Since the beginning of the 20th century, modernism introduced to the world an architectural composite that consists of concrete, steel and glass. Heading into the 21st century, the use of these three materials has only expanded as it continues to be the most economically efficient means of construction. While digital technology in design and construction continues to evolve, the materials at which we construct architecture has remained the same. Given the rapid growth of the human population, new and more sustainable approaches to construction methodologies and materials need to be explored and utilised. This research will demonstrate the potential of freeform 3D printing as a sustainable and efficient alternative building method. It outlines contemporary digital design techniques including computation and simulation tools as a means to define and test this proposed building method including structural optimisation tools to create the most structurally efficient form from additive manufacturing. The computational methods described are then applied to a manufacturing process that includes a 6-axis robotic arm. The final result is a building methodology that supports a computational workflow from design conception to manufacture.</p>
Livy's Witch-hunts: A Study of Investigations into Veneficium found in Livy
Authors:   , Grandy Liam 
Published: December 2021

<p>This thesis is an exploration of large scale incidents of veneficium as they are depicted in Livy’s Ab Urbe Condita. Livy’s earlier books include references to four quaestiones de veneficiis, investigations into poisoning, which resulted in the executions of thousands of people. This study attempts to understand what happened while hypothesising why they occurred. Veneficium and its associated words have often been declared ambiguous, referring to poisons, potions, and, eventually, magic. However, this interpretation developed significantly later than the events seen in Livy and is anachronistic. This thesis explores this language and so we can understand what veneficium meant during the quaestiones de veneficiis of the fourth and second centuries BC and in Livy’s own time, and how it evolved to become magical and thus colour modern scholarships. Using this knowledge, we can review and reconsider Livy’s reports to gain a fresh understanding of what actually happened during the quaestiones and how the motifs and themes of these investigations reveal that they were in fact social responses to a period of rapid change to Roman life in the second century BC. This final point is reaffirmed when we engage with interdisciplinary theories from anthropology and sociology. By considering theories and models from these schools we can confidently say that, while venefici were not witches, their persecution was a type of witch-hunt.</p>
Isolation and Characterisation of Toxic Secondary Metabolites Produced by Black Mould
Authors:   , Hudson Daniel 
Published: December 2021

<p>Microbial secondary metabolites, commonly called natural products, have been crucial for the progression of modern medicine. Not essential for the basic functions of life, secondary metabolites are instead produced to provide a competitive advantage in the environment. The method of action is commonly toxicity to other species in their environment, thereby harming or killing the competition. These toxic properties have allowed them to be utilised as antimicrobial and antitumor agents, however this same toxicity is able to cause detrimental health effects in humans causing symptoms ranging from minor to life threatening. The black mould Stachybotrys chartarum is capable of producing very toxic secondary metabolites called macrocyclic trichothecenes. Satratoxin G (6) and H (7), are two of the most toxic naturally occurring compounds in the world. This has made S. chartarum a common target when adverse health has been associated with damp and mouldy dwellings. However, there is very little evidence for this link beyond its ubiquity and ability to produce the aforementioned highly toxic macrocyclic trichothecenes. This research investigates S. chartarum and the toxic secondary metabolites it produces, with special emphasis on satratoxin G and H. Different culturing methods and resulting morphology are assessed. The satratoxins were isolated from crude extracts and full characterisation by 1-D and 2-D NMR spectroscopy was done. This process revealed differences from the accepted literature, and spectra are reported herein to aid in future identification. The importance of genetics and the public health implications of mould contamination are also discussed.</p>