bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comparative analysis of mite genomes reveals positive selection for diet adaptation
Authors: Liu Qiong , Deng Yuhua , Song An , Xiang Yifan , Chen De , Wei Lai 
Published: June 2021

AbstractDiet is a powerful evolutionary force for species adaptation and diversification. Acari is one of the most abundant clades of Arachnida, exhibiting diverse dietary types, while the underlying genetic adaptive mechanisms are not fully understood. Based on comparative analyses of 15 Acari genomes, we found genetic bases for three specialized diets. Herbivores experienced stronger selection pressure than other groups; the olfactory genes and gene families involving metabolizing toxins showed strong adaptive signals. Genes and gene families related to anticoagulation, detoxification, and haemoglobin digestion were found to be under strong selection pressure or significantly expanded in the blood-feeding species. Lipid metabolism genes have a faster evolutionary rate and been subjected to greater selection pressures in fat-feeding species; one positively selected site in the fatty-acid amide hydrolases 2 gene was identified. Our research provides a new perspective for the evolution of Acari and offers potential target loci for novel pesticide development.
The chaperonin CCT8 controls proteostasis essential for T cell maturation, selection, and function
Authors: Oftedal Bergithe E. , Maio Stefano , Handel Adam E. , White Madeleine P. J. , Howie Duncan , Davis Simon , Prevot Nicolas , Rota Ioanna A. , Deadman Mary E. , Kessler Benedikt M. , Fischer Roman , Trede Nikolaus S. , Sezgin Erdinc , Maizels Rick M. , Holländer Georg A. 
Published: June 2021

AbstractT cells rely for their development and function on the correct folding and turnover of proteins generated in response to a broad range of molecular cues. In the absence of the eukaryotic type II chaperonin complex, CCT, T cell activation induced changes in the proteome are compromised including the formation of nuclear actin filaments and the formation of a normal cell stress response. Consequently, thymocyte maturation and selection, and T cell homeostatic maintenance and receptor-mediated activation are severely impaired. In the absence of CCT-controlled protein folding, Th2 polarization diverges from normal differentiation with paradoxical continued IFN-γ expression. As a result, CCT-deficient T cells fail to generate an efficient immune protection against helminths as they are unable to sustain a coordinated recruitment of the innate and adaptive immune systems. These findings thus demonstrate that normal T cell biology is critically dependent on CCT-controlled proteostasis and that its absence is incompatible with protective immunity.
Glucocorticoid receptor wields chromatin interactions to tune transcription for cytoskeleton stabilization in podocytes
Authors: Wang Hong , Duan Aiping , Zhang Jing , Wang Qi , Xing Yuexian , Qin Zhaohui , Liu Zhihong , Yang Jingping 
Published: June 2021

AbstractElucidating transcription mediated by the glucocorticoid receptor (GR) is crucial for understanding the role of glucocorticoids (GCs) in the treatment of diseases. Podocyte is a useful model for studying GR regulation because GCs are the primary medication for podocytopathy. In this study, we integrated data from transcriptome, transcription factor binding, histone modification, and genome topology. Our data reveals that the GR binds and activates selective regulatory elements in podocyte. The 3D interactome captured by HiChIP facilitates the identification of remote targets of GR. We found that GR in podocyte is enriched at transcriptional interaction hubs and super-enhancers. We further demonstrate that the target gene of the top GR-associated super-enhancer is indispensable to the effective functioning of GC in podocyte. Our findings provided insights into the mechanisms underlying the protective effect of GCs on podocyte, and demonstrate the importance of considering transcriptional interactions in order to fine-map regulatory networks of GR.
Zinc isotopes from archaeological bones provide reliable trophic level information for marine mammals
Authors: McCormack Jeremy , Szpak Paul , Bourgon Nicolas , Richards Michael , Hyland Corrie , Méjean Pauline , Hublin Jean-Jacques , Jaouen Klervia 
Published: June 2021

AbstractIn marine ecology, dietary interpretations of faunal assemblages often rely on nitrogen isotopes as the main or only applicable trophic level tracer. We investigate the geographic variability and trophic level isotopic discrimination factors of bone zinc 66Zn/64Zn ratios (δ66Zn value) and compared it to collagen nitrogen and carbon stable isotope (δ15N and δ13C) values. Focusing on ringed seals (Pusa hispida) and polar bears (Ursus maritimus) from multiple Arctic archaeological sites, we investigate trophic interactions between predator and prey over a broad geographic area. All proxies show variability among sites, influenced by the regional food web baselines. However, δ66Zn shows a significantly higher homogeneity among different sites. We observe a clear trophic spacing for δ15N and δ66Zn values in all locations, yet δ66Zn analysis allows a more direct dietary comparability between spatially and temporally distinct locations than what is possible by δ15N and δ13C analysis alone. When combining all three proxies, a more detailed and refined dietary analysis is possible.
Phenotypic analysis of catastrophic childhood epilepsy genes
Authors: Griffin Aliesha , Carpenter Colleen , Liu Jing , Paterno Rosalia , Grone Brian , Hamling Kyla , Moog Maia , Dinday Matthew T. , Figueroa Francisco , Anvar Mana , Ononuju Chinwendu , Qu Tony , Baraban Scott C. 
Published: June 2021

AbstractGenetic engineering techniques have contributed to the now widespread use of zebrafish to investigate gene function, but zebrafish-based human disease studies, and particularly for neurological disorders, are limited. Here we used CRISPR-Cas9 to generate 40 single-gene mutant zebrafish lines representing catastrophic childhood epilepsies. We evaluated larval phenotypes using electrophysiological, behavioral, neuro-anatomical, survival and pharmacological assays. Local field potential recordings (LFP) were used to screen ∼3300 larvae. Phenotypes with unprovoked electrographic seizure activity (i.e., epilepsy) were identified in zebrafish lines for 8 genes; ARX, EEF1A, GABRB3, GRIN1, PNPO, SCN1A, STRADA and STXBP1. We also created an open-source database containing sequencing information, survival curves, behavioral profiles and representative electrophysiology data. We offer all zebrafish lines as a resource to the neuroscience community and envision them as a starting point for further functional analysis and/or identification of new therapies.
Templated interfacial synthesis of metal-organic framework (MOF) nano- and micro-structures with precisely controlled shapes and sizes
Authors: Meng Lingyao , Yu Binyu , Qin Yang 
Published: June 2021

AbstractMetal-organic frameworks (MOF) are an emerging class of microporous materials with promising applications. MOF nanocrystals, and their assembled super-structures, can display unique properties and reactivities when compared with their bulk analogues. MOF nanostructures of 0-D, 2-D, and 3-D dimensions can be routinely obtained by controlling reaction conditions and ligand additives, while formation of 1-D MOF nanocrystals (nanowires and nanorods) and super-structures has been relatively rare. We report here a facile templated interfacial synthesis methodology for the preparation of a series of 1-D MOF nano- and micro-structures with precisely controlled shapes and sizes. Specifically, by applying track-etched polycarbonate (PCTE) membranes as the templates and at the oil/water interface, we rapidly and reproducibly synthesize zeolitic imidazolate framework-8 (ZIF-8) and ZIF-67 nano- and micro structures of sizes ranging from 10 nm to 20 μm. We also identify a size confinement effect on MOF crystal growth, which leads to single crystals under the most restricted conditions and inter-grown polycrystals at larger template pore sizes, as well as surface directing effects that influence the crystallographic preferred orientation. Our findings provide a potentially generalizable method for controlling the size, morphology, and crystal orientations of MOF nanomaterials, as well as offering fundamental understanding into MOF crystal growth mechanisms.
Merohedral disorder and impurity impacts on superconductivity of fullerenes
Authors: Wang Shu-Ze , Ren Ming-Qiang , Han Sha , Cheng Fang-Jun , Ma Xu-Cun , Xue Qi-Kun , Song Can-Li 
Published: June 2021

AbstractLocal quasiparticle states around impurities provide essential insight into the mechanism of unconventional superconductivity, especially when the candidate materials are proximate to an antiferromagnetic Mott-insulating phase. While such states have been reported in atom-based cuprates and iron-based compounds, they are unexplored in organic superconductors which feature tunable molecular orientation. Here we employ scanning tunneling microscopy and spectroscopy to reveal multiple forms of robustness of an exotic s-wave superconductivity in epitaxial Rb3C60 films against merohedral disorder, non-magnetic single impurities and step edges at the atomic scale. Yu-Shiba-Rusinov (YSR) states, induced by deliberately incurred Fe adatoms that act as magnetic scatterers, have also been observed. The YSR bound states show abrupt spatial decay and vary in energy with the Fe adatom registry. These results and a doping-dependent study of superconductivity point towards local electron pairing in which the multiorbital electronic correlations and intramolecular phonons together drive the high-temperature superconductivity of doped fullerenes.
Tunable polarization-induced Fano resonances in stacked wire-grid metasurfaces
Authors: Romain Xavier , Degl’Innocenti Riccardo , Baida Fadi I. , Boyer Philippe 
Published: June 2021

AbstractStacked metasurfaces are being investigated in light of exploring exotic optical effects that cannot be achieved with single-layered metasurfaces. In this article, we theoretically demonstrate that metallic wire-grid metasurfaces with specific polarization properties have the ability to induce tunable Fano resonances when they are stacked. The developed original model—combining a circulating field approach together with an extended Jones formalism—reveals the underlying principle that gives rise to the polarization-induced Fano resonances. The theoretical frame is validated in an experimental proof of concept using commercially available wire-grids and a terahertz time domain spectrometer. This unexplored possibility opens an alternative path to the realization and control of Fano resonances by using stacked metallic metasurfaces. Furthermore, these findings suggest that the polarization can be used as an additional degree of freedom for the design of optical resonators with enhanced and tunable properties.
Biosignatures of ancient microbial life are present across the igneous crust of the Fennoscandian shield
Authors: Drake Henrik , Roberts Nick M. W. , Reinhardt Manuel , Whitehouse Martin , Ivarsson Magnus , Karlsson Andreas , Kooijman Ellen , Kielman-Schmitt Melanie 
Published: June 2021

AbstractEarth’s crust contains a substantial proportion of global biomass, hosting microbial life up to several kilometers depth. Yet, knowledge of the evolution and extent of life in this environment remains elusive and patchy. Here we present isotopic, molecular and morphological signatures for deep ancient life in vein mineral specimens from mines distributed across the Precambrian Fennoscandian shield. Stable carbon isotopic signatures of calcite indicate microbial methanogenesis. In addition, sulfur isotope variability in pyrite, supported by stable carbon isotopic signatures of methyl-branched fatty acids, suggest subsequent bacterial sulfate reduction. Carbonate geochronology constrains the timing of these processes to the Cenozoic. We suggest that signatures of an ancient deep biosphere and long-term microbial activity are present throughout this shield. We suggest that microbes may have been active in the continental igneous crust over geological timescales, and that subsurface investigations may be valuable in the search for extra-terrestrial life.
Central Asian modulation of Northern Hemisphere moisture transfer over the Late Cenozoic
Authors: Prud’homme Charlotte , Scardia Giancarlo , Vonhof Hubert , Guinoiseau Damien , Nigmatova Saida , Fiebig Jens , Gerdes Axel , Janssen Renee , Fitzsimmons Kathryn E. 
Published: June 2021

AbstractEarth’s climatic evolution over the last 5 million years is primarily understood from the perspective of marine mechanisms, however, the role of terrestrial feedbacks remains largely unexplored. Here we reconstruct the last 5 million years of soil moisture variability in Central Asia using paleomagnetism data and isotope geochemistry of an 80 m-thick sedimentary succession at Charyn Canyon, Kazakhstan. We identify a long-term trend of increasing aridification throughout the period, along with shorter-term variability related to the interaction between mid-latitude westerlies and the Siberian high-pressure system. This record highlights the long-term contribution of mid-latitude Eurasian terrestrial systems to the modulation of moisture transfer into the Northern Hemisphere oceans and back onto land via westerly air flow. The response of Earth-surface dynamics to Plio-Pleistocene climatic change in Central Asia likely generated terrestrial feedbacks affecting ocean and atmospheric circulation. This missing terrestrial link elucidates the significance of land-water feedbacks for long-term global climate.
Creating a Path for Systematic Investigation of Children With Cortical Visual Impairment Who Use Augmentative and Alternative Communication
Authors: Boster Jamie B. , McCarthy John W. , Brown Kathryn , Spitzley Alyson M. , Blackstone Sarah W. 
Published: June 2021

Purpose
There is limited information about how to support children with cortical visual impairment (CVI) who require augmentative and alternative communication (AAC). An initial review designed to explore the available evidence was used to outline critical needs in moving research and intervention forward for children who use AAC and have CVI.

Method
Previous systematic reviews, six databases, and theses and dissertations were systematically searched, along with reviews of the resulting works cited. An initial yield of 575 articles was narrowed to 10, which discussed AAC interventions that included children with CVI.

Results
Three interventions were technology based, and seven were instructional based. The use of textured microswitches was the most frequent form of technology intervention, with the frequency of switch activations being the most frequently coded outcome. Overall, the studies represent explorations in the area rather than systematic lines of inquiry.

Conclusions
While evidence shows at least some children with CVI have been included in AAC research to date, the inclusion is more incidental than deliberate. Issues such as clear descriptions of the vision capabilities and needs of participants may have impacted study results.
High efficiency low thermal resistance semiconductor laser with novel insulating layer and coating film
Authors: Wang Yuancheng , Qi Aiyi , Wang Yufei , Qu Hongwei , Zheng Wanhua 
Published: June 2021

AbstractThermal effect of semiconductor lasers is the biggest challenge to the development of semiconductor lasers. This problem limits the life and performance of high‐power laser diode. In this letter, we have reduced the thermal resistance of laser to enhance its heat dissipation capability. For this, we choose aluminium nitride (AlN) to replace traditional materials as insulating layer, as well as a passivation film for facet coating. The impact of insulation layer and facet coating on the thermal rollover process is analyzed. Finite element method simulations are compared with the experimental result. The comparison shows that the thermal resistance of the laser with the new insulating layer is reduced compared with normal laser diodes (LD). Eventually, the maximum conversion efficiency is 67.5% at room temperature and thermal resistance is 1.81C° W–1.
Expectant Management of Placenta Accreta Spectrum Disorders
Authors: Kondoh Eiji 
Published: June 2021

AbstractPlacenta accreta spectrum (PAS) disorder is a potentially life-threatening complication. The incidence of PAS has increased over the past few decades, mainly due to the increased cesarean section rate. While cesarean hysterectomy remains the most standard treatment for the management of PAS, expectant management is becoming increasingly prevalent to avoid serious maternal morbidity and maintain future fertility. Expectant management is defined as leaving the placenta either partially or fully in situ, and waiting for its spontaneous resorption or expulsion. The success rate of expectant management is high, but intraoperative uncontrolled hemorrhage results in hysterectomy. Moreover, some individuals fail expectant management and require delayed hysterectomy due to complications such as secondary postpartum hemorrhage, sepsis, uterine necrosis, and vesicouterine fistula. As a result of the very limited data currently available, there is no consensus on the optimal strategy for the expectant management of PAS. However, it is clear that a multidisciplinary team approach in tertiary centers is essential for women with PAS. In addition, meticulous preparation is the key to successful expectant management. Here, we describe a surgical strategy designed to reduce perioperative blood loss, which is a minimum requirement to ensure maternal safety. This article also addresses practical issues in expectant management of PAS, based on the published literature and our own experience.
Aspirin Is Associated with Decreased Allogeneic Transfusions and Resource Utilization following Hip Fracture Care
Authors: Anoushiravani Afshin A. , Sayeed Zain , Mori Benjamin V. , Padela Muhammad T. , Barinaga Gonzalo , Cagle Paul J. , Saleh Khaled J. 
Published: June 2021

AbstractThe purpose of our study is to assess the relationship and compare the impact of aspirin, enoxaparin, and warfarin use on postoperative anemia, allogenic transfusions, and resource utilization following hip fracture fixation. This is a retrospective study at a Level 1 trauma center with 450 geriatric hip fracture patients who underwent hip fracture surgery and chemoprophylaxis. Hip fracture patients were separated into three cohorts depending on the type of chemoprophylaxis administered aspirin, enoxaparin, or warfarin. Initially, all three cohorts were assessed for baseline characteristics, postoperative anemia, transfusion rates, and resource utilization. Next, aspirin and enoxaparin were comparatively evaluated for the same variables. Four hundred and fifty patients met inclusion criteria for the first portion of this study. No baseline variance was evident among the three cohorts except for body mass index (p = 0.007) and diagnosis of congestive heart failure (p = 0.001). Outcomes were insignificant for in-hospital mortality (p = 0.19), postoperative anemia (p = 0.43), hemoglobin levels (p = 0.91), and ∆hemoglobin (p = 0.99), length-of-stay (p = 0.12), disposition (p = 0.13), and 30-day readmission (p =0.09). The transfusion rate (p < 0.001) and hospital cost (p = 0.01) varied significantly among the prophylactic cohorts. Three hundred and eighty nine patients met inclusion criteria for the aspirin and enoxaparin comparison. Baseline characteristics between the cohorts only revealed a significant variance for age (p = 0.03). Outcomes did not vary significantly among aspirin and enoxaparin cohorts; however, hospital cost was 12.3% greater in patients receiving enoxaparin (p = 0.01). Our study compares administration of aspirin, warfarin, and enoxaparin as means of chemoprophylaxis following hip fracture repair. Analyses of outcomes demonstrated patients receiving warfarin were more likely to require transfusions. Additionally, when compared with aspirin, resource utilization was 16.9 and 12.3% greater with warfarin and enoxaparin, respectively. Thus, our study suggests that aspirin is a safe and cost-effective option for chemoprophylaxis following hip fracture fixation.
Hand Masses
Authors: Teh James , Shahabpour Maryam , Drape Jean-Luc , Feydy Antoine , Sudoł-Szopińska Iwona , Vanhoenacker Filip M. 
Published: June 2021

AbstractHand and wrist soft tissue masses may be classified as pseudotumors, benign neoplasms, or malignant neoplasms. The vast majority of hand lesions are benign. Consideration of the location of the lesion and its imaging characteristics often leads to a specific diagnosis. Pseudotumors discussed in this article are ganglion cysts, accessory muscles, and inflammatory lesions. True tumors are described according to their tissue type: nerve sheath tumors, adipocytic tumors, so-called fibrohistiocytic tumors, pericytic tumors, and vascular lesions. We also outline the imaging features of masses encountered in the hand and wrist.
Ambulatory Phlebectomy and Sclerotherapy as Tools for the Treatment of Varicose Veins and Telangiectasias
Authors: Andrews R. Hampton , Dixon Robert G. 
Published: June 2021

AbstractTreatment of chronic venous disease is a fascinating and rewarding undertaking. Once the truncal reflux is addressed, several options are available that can be used to treat the associated ulcers, varicosities, reticular veins, and telangiectasias. This review will focus on two widely employed procedures: ambulatory phlebectomy and sclerotherapy.
Evaluation and Management of Patients with Leg Swelling: Therapeutic Options for Venous Disease and Lymphedema
Authors: Scherer Kimberly , Khilnani Neil 
Published: June 2021

AbstractLower extremity swelling is a common condition which has a variety of etiologies and can be challenging to diagnose and manage. Swelling is usually the result of the accumulation of interstitial fluid in the subcutaneous tissues. Common etiologies include systemic, superficial, and deep venous, and lymphatic disorders. Leg swelling can occur bilaterally or unilaterally, with venous disorders being one of the most common causes of unilateral lower extremity edema.
The total number and mass of SARS-CoV-2 virions
Authors: Sender Ron , Bar-On Yinon M. , Gleizer Shmuel , Bernshtein Biana , Flamholz Avi , Phillips Rob , Milo Ron 
Published: June 2021

Significance
Knowing the absolute numbers of virions in an infection promotes better understanding of disease dynamics and response of the immune system. Here we use current knowledge on the concentrations of virions in infected individuals to estimate the total number and mass of SARS-CoV-2 virions in an infected person. Although each infected person carries an estimated 1 billion to 100 billion virions during peak infection, their total mass is no more than 0.1 mg. This curiously implies that all SARS-CoV-2 virions currently in all human hosts have a mass of between 100 g and 10 kg. Combining the known mutation rate and our estimate of the number of infectious virions, we quantify the formation rate of genetic variants.