bluuebunny/crossref_metadata_2025_split huggingface dataset:
Blood gas phenotyping and tracheal intubation timing in adult in-hospital cardiac arrest: a retrospective cohort study
Authors: Wang Chih-Hung , Wu Meng-Che , Wu Cheng-Yi , Huang Chien-Hua , Tsai Min-Shan , Lu Tsung-Chien , Chou Eric , Wu Yen-Wen , Chang Wei-Tien , Chen Wen-Jone 
Published: May 2021

AbstractTo investigate whether the optimal time to tracheal intubation (TTI) during cardiopulmonary resuscitation would differ by different blood gas phenotypes. Adult patients experiencing in-hospital cardiac arrest (IHCA) from 2006 to 2015 were retrospectively screened. Early intra-arrest blood gas analysis, performed within 10 min of resuscitation, was used to define different phenotypes. In total, 567 patients were included. Non-severe acidosis (pH≧7.15) was associated with favourable neurological outcome (odds ratio [OR]: 4.60, 95% confidence interval [CI] 1.63–12.95; p value = 0.004) and survival (OR: 3.25, 95% CI 1.72–6.15; p value < 0.001) in the multivariable logistic regression analyses. In the interaction analysis, normal blood gas phenotype (pH: 7.35–7.45, PCO2: 35–45 mm Hg, HCO3− level: 22–26 mmol/L) × TTI ≦ 6.3 min (OR: 20.40, 95% CI 2.53–164.75; p value = 0.005) and non-severe acidosis × TTI ≦ 6.3 min (OR: 3.35, 95% CI 1.00–11.23; p value = 0.05) were associated with neurological recovery while metabolic acidosis × TTI ≦ 5.7 min (OR: 3.63, 95% CI 1.36–9.67; p value = 0.01) and hypercapnic acidosis × TTI ≦ 10.4 min (OR: 2.27, 95% CI 1.20–4.28; p value = 0.01) were associated with survival. Intra-arrest blood gas analysis may help guide TTI during for patients with IHCA.
Egg-trading worms start reciprocation with caution, respond with confidence and care about partners’ quality
Authors: Lorenzi Maria Cristina , Schleicherová Dáša , Robles-Guerrero Franco G. , Dumas Michela , Araguas Alice 
Published: May 2021

AbstractConditional reciprocity (help someone who helped you before) explains the evolution of cooperation among unrelated individuals who take turns helping each other. Reciprocity is vulnerable to exploitations, and players are expected to identify uncooperative partners who do not return the help they received. We tested this prediction in the simultaneously hermaphroditic worm, Ophryotrocha diadema, which engages in mutual egg donations by alternating sexual roles (one worm releases’ eggs and the other fertilizes them). We set up dyads with different cooperativeness expectations; partners were either the same or a different body size (body size predicts clutch size). Large worms offered larger clutches and did so sooner when paired with large rather than small partners. They also released smaller egg clutches when they started egg donations than when they responded to a partners’ donation, fulfilling the prediction that a players’ first move will be prudent. Finally, behavioral bodily interactions were more frequent between more size-dissimilar worms, suggesting that worms engaged in low-cost behavioral exchanges before investing in such costly moves as egg donations. These results support the hypothesis that simultaneously hermaphroditic worms follow a conditional reciprocity paradigm and solve the conflict over sexual roles by sharing the costs of reproduction via the male and the female functions.
G protein-coupled receptor kinase 5 deletion suppresses synovial inflammation in a murine model of collagen antibody-induced arthritis
Authors: Toya Masakazu , Akasaki Yukio , Sueishi Takuya , Kurakazu Ichiro , Kuwahara Masanari , Uchida Taisuke , Tsutsui Tomoaki , Tsushima Hidetoshi , Yamada Hisakata , Lotz Martin K. , Nakashima Yasuharu 
Published: May 2021

AbstractG protein-coupled receptor kinase 5 (GRK5) regulates inflammatory responses via the nuclear factor-kappa B (NF-κB) pathway. This study investigated the functional involvement of GRK5 in the pathogenesis of inflammatory arthritis. Immunohistochemically, rheumatoid arthritis (RA) synovium had a significantly higher proportion of GRK5-positive cells in the synovial lining layer than healthy control synovium. Gene expression and NF-κB activation in lipopolysaccharide-stimulated human SW982 synovial cells were significantly suppressed by silencing of the GRK5 gene. Similarly, GRK5 kinase activity inhibition in human primary RA synovial cells attenuated gene expressions of inflammatory factors. In a murine model of collagen antibody-induced arthritis, arthritis scores and serum IL6 production of GRK5 knockout (GRK5-/-) mice were significantly lower than those of wild-type mice. Histologically, the degree of synovitis and cartilage degeneration in GRK5-/- mice was significantly lower than in wild-type mice. In in vitro analyses using activated murine macrophages and fibroblast-like synoviocytes, gene expression of inflammatory factors and p65 nuclear translocation were significantly lower in GRK5-/- mice compared to wild-type mice. In conclusion, our results suggested that GRK5 is deeply involved in the pathogenesis of inflammatory arthritis, therefore, GRK5 inhibition could be a potential therapeutic target for types of inflammatory arthritis such as RA.
High-grade extracellular vesicles preparation by combined size-exclusion and affinity chromatography
Authors: Bellotti Cristina , Lang Kristina , Kuplennik Nataliya , Sosnik Alejandro , Steinfeld Robert 
Published: May 2021

AbstractExtracellular vesicles (EVs) have recently gained growing interest for their diagnostic and therapeutic potential. Despite this, few protocols have been reported for the isolation of EVs with preserved biological function. Most EV purification methods include a precipitation step that results in aggregation of vesicles and most available techniques do not efficiently separate the various types of EVs such as exosomes and ectosomes, which are involved in distinct biological processes. For this reason, we developed a new two-step fast performance liquid chromatography (FPLC) protocol for purification of large numbers of EVs. The method comprises size exclusion chromatography followed by immobilized metal affinity chromatography, which is enabled by expression of poly-histidine tagged folate receptor α in the parental cells. Characterisation and comparison of the EVs obtained by this method to EVs purified by differential centrifugation, currently the most common method to isolate EVs, demonstrated higher purity and more selective enrichment of exosomes in EV preparations using our FPLC method, as assessed by comparison of marker proteins and density distribution. Our studies reveal new possibilities for the isolation of defined subpopulations of EVs with preserved biological function that can easily be upscaled for production of larger amounts of EVs.
Relation between preoperative benzodiazepines and opioids on outcomes after total joint arthroplasty
Authors: Doan Lisa V. , Padjen Kristoffer , Ok Deborah , Gover Adam , Rashid Jawad , Osmani Bijan , Avraham Shirley , Wang Jing , Kendale Samir 
Published: May 2021

AbstractTo examine the association of preoperative opioids and/or benzodiazepines on postoperative outcomes in total knee and hip arthroplasty, we retrospectively compared postoperative outcomes in those prescribed preoperative opioids and/or benzodiazepines versus those who were not who underwent elective total knee and hip arthroplasty at a single urban academic institution. Multivariable logistic regression was performed for readmission rate, respiratory failure, infection, and adverse cardiac events. Multivariable zero-truncated negative binomial regression was used for length of stay. After exclusions, there were 4307 adult patients in the study population, 2009 of whom underwent total knee arthroplasty and 2298 of whom underwent total hip arthroplasty. After adjusting for potential confounders, preoperative benzodiazepine use was associated with increased odds of readmission (p < 0.01). Preoperative benzodiazepines were not associated with increased odds of respiratory failure nor increased length of stay. Preoperative opioids were not associated with increased odds of the examined outcomes. There were insufficient numbers of infection and cardiac events for analysis. In this study population, preoperative benzodiazepines were associated with increased odds of readmission. Preoperative opioids were not associated with increased odds of the examined outcomes. Studies are needed to further examine risks associated with preoperative benzodiazepine use.
Evaluation of the Idylla ctEGFR mutation assay to detect EGFR mutations in plasma from patients with non-small cell lung cancers
Authors: Gilson Pauline , Saurel Chloé , Salleron Julia , Husson Marie , Demange Jessica , Merlin Jean-Louis , Harlé Alexandre 
Published: May 2021

AbstractThe assessment of EGFR mutations is recommended for the management of patients with non-small cell lung cancer (NSCLC). Presence of EGFR mutation is associated with response or resistance to EGFR tyrosine kinase inhibitors (EGFR-TKI). Liquid biopsy is nowadays widely used for the detection of resistance to EGFR-TKI. We evaluated here the performance of the Idylla ctEGFR mutation assay for the detection of EGFR mutations in circulating tumour DNA (ctDNA) in plasma from patients with NSCLC. Previously characterized plasma samples from 38 patients with NSCLC were analysed using 2 different analytical conditions (C1 and C2). The limit of detection (LOD) was evaluated using 2 mL of healthy donor plasma spiked with commercial DNA controls. Overall agreement, sensitivity and specificity were 92.1%, 86.7% and 95.7% for C1 condition respectively and 94.7%, 86.7% and 100% for C2 condition respectively. The T790M secondary resistance mutation was detected in two samples out of 3. The Idylla system was able to detect the exon 19 deletion from 6 copies/mL and up to 91 copies/mL for the G719S mutation. These results support that the Idylla ctEGFR mutation assay is a rapid option for the detection of EGFR hotspots mutations in plasma samples, however a particular attention is needed for its interpretation.
High glucose–induced Smad3 linker phosphorylation and CCN2 expression are inhibited by dapagliflozin in a diabetic tubule epithelial cell model
Authors: Pan Xinlu , Phanish Mysore K. , Baines Deborah L. , Dockrell Mark E.C. 
Published: May 2021

AbstractBackground: In the kidney glucose is freely filtered by the glomerulus and, mainly, reabsorbed by sodium glucose cotransporter 2 (SGLT2) expressed in the early proximal tubule. Human proximal tubule epithelial cells (PTECs) undergo pathological and fibrotic changes seen in diabetic kidney disease (DKD) in response to elevated glucose. We developed a specific in vitro model of DKD using primary human PTECs with exposure to high D-glucose and TGF-β1 and propose a role for SGLT2 inhibition in regulating fibrosis. Methods: Western blotting was performed to detect cellular and secreted proteins as well as phosphorylated intracellular signalling proteins. qPCR was used to detect CCN2 RNA. Gamma glutamyl transferase (GT) activity staining was performed to confirm PTEC phenotype. SGLT2 and ERK inhibition on high D-glucose, 25 mM, and TGF-β1, 0.75 ng/ml, treated cells was explored using dapagliflozin and U0126, respectively. Results: Only the combination of high D-glucose and TGF-β1 treatment significantly up-regulated CCN2 RNA and protein expression. This increase was significantly ameliorated by dapagliflozin. High D-glucose treatment raised phospho ERK which was also inhibited by dapagliflozin. TGF-β1 increased cellular phospho SSXS Smad3 serine 423 and 425, with and without high D-glucose. Glucose alone had no effect. Smad3 serine 204 phosphorylation was significantly raised by a combination of high D-glucose+TGF-β1; this rise was significantly reduced by both SGLT2 and MEK inhibition. Conclusions: We show that high D-glucose and TGF-β1 are both required for CCN2 expression. This treatment also caused Smad3 linker region phosphorylation. Both outcomes were inhibited by dapagliflozin. We have identified a novel SGLT2 -ERK mediated promotion of TGF-β1/Smad3 signalling inducing a pro-fibrotic growth factor secretion. Our data evince support for substantial renoprotective benefits of SGLT2 inhibition in the diabetic kidney.
Deleterious mutation V369M in the mouse GCGR gene causes abnormal plasma amino acid levels indicative of a possible liver–α-cell axis
Authors: Liu Qiaofeng , Lin Guangyao , Chen Yan , Feng Wenbo , Xu Yingna , Lyu Jianjun , Yang Dehua , Wang Ming-Wei 
Published: May 2021

Abstract
Glucagon plays an important role in glucose homeostasis and amino acid metabolism. It regulates plasma amino acid levels which in turn modulate glucagon secretion from the pancreatic α-cell, thereby establishing a liver–α-cell axis described recently. We reported previously that the knock-in mice bearing homozygous V369M substitution (equivalent to a naturally occurring mutation V368M in the human glucagon receptor, GCGR) led to hypoglycemia with improved glucose tolerance. They also exhibited hyperglucagonemia, pancreas enlargement and α-cell hyperplasia. Here, we investigated the effect of V369M/V368M mutation on glucagon-mediated amino acid metabolism. It was found that GcgrV369M+/+ mice displayed increased plasma amino acid levels in general, but significant accumulation of the ketogenic/glucogenic amino acids was observed in animals fed with a high-fat diet (HFD), resulting in deleterious metabolic consequence characteristic of α-cell proliferation and hyperglucagonemia.
Mode‐dependent switching control of bilateral teleoperation against random denial‐of‐service attacks
Authors: Hu Lingyan , Wang Kun , Hu Dongxia , Wang Yiming 
Published: May 2021

AbstractAs communication networks are implemented for information exchange between the master and slave sides of bilateral teleoperation systems, they are exposed to cyber‐attack threats. This paper aims to analyse the performance of bilateral teleoperation systems in the presence of random denial‐of‐service (DoS) attacks and constant transmission delays and propose a mode‐dependent switching controller to mitigate the influence of DoS attacks. The characteristics of DoS attacks and networks are thoroughly incorporated in the design; also considered is the case of both communication directions behaving independently. Specifically, the model of a teleoperation system under a DoS attack is integrated as a stochastic jump system. A mode‐dependent control approach is proposed for a teleoperation system to mitigate the influence of random DoS attacks. In case studies, vulnerability analysis and time‐domain simulation results show that teleoperation system performance can be degraded under continuous random DoS attacks. When the proposed mode‐based switching controllers are installed, the trajectory tracking performance and authenticity of interaction force feedback are significantly improved during the attacking period.
Polarimetric HRRP recognition based on feature‐guided Transformer model
Authors: Zhang Liang , Han Chang , Wang Yanhua , Li Yang , Long Teng 
Published: May 2021

AbstractPolarimetric high‐resolution range profile (HRRP) holds great potential for radar automatic target recognition (RATR) owing to its capability of providing both polarimetric and spatial scattering information. In recent years, deep learning (DL) has obtained state‐of‐the‐art results in many classification tasks and has drawn great attention in the RATR field. However, as one of the most challenging tasks in RATR, small training sample case will restrict the application of DL because its superior performance generally depends on a large number of training samples. A feature‐guided deep model based on Transformer framework is proposed for polarimetric HRRP recognition with limited training samples. In the proposed model, artificial features are introduced to the attention module to guide the model focus on the range cells of HRRP with more target scattering information so as to reduce the dependence of the model on the number of training samples. Several different approaches are also studied to measure the similarity between artificial features and HRRP data to further improve the learning capacity of the model. Experimental results demonstrate that the proposed feature‐guided Transformer model modifying by Cosine similarity measure is able to achieve a better performance for polarimetric HRRP recognition with limited training samples.
Temperature‐dependent partial discharge characteristics of high temperature materials at DC voltage for hybrid propulsion systems
Authors: Shahsavarian Tohid , Wu Xin , Lents Charles , Zhang Di , Li Chuanyang , Cao Yang 
Published: May 2021

AbstractThe safe and reliable operation of insulation material used in key high voltage components under extreme environmental conditions represents the major concerns for manufacturers and operators of More Electric Aircrafts (MEA). Surface discharge occurring in high current carrying components in DC power system diminishes the insulation material’s performance and life, especially at high‐temperature conditions. Here, the surface discharge behaviour of two commonly used high‐temperature insulation materials, ethylene‐tetrafluoroethylene (ETFE) and polyetheretherketone (PEEK) is studied at different temperatures under ramp and DC voltages. Extracted partial discharge (PD) features are presented and the impact of voltage polarity on surface discharge propagation is discussed. Our studies reveal that, while both materials exhibit non‐linear PD behaviour with respect to their electrical conductivity, ETFE generally shows PDs with higher intensity at high temperature above 100°C with a higher possibility of surface discharge due to its lower permittivity. Overall, the PD mechanism in high‐temperature, DC voltage applications is explored, and a basis for the selection of high‐temperature PD suppressing materials is developed.
A two‐stage multi microgrids p2p energy trading with motivational game‐theory: A case study in malaysia
Authors: Yap Yu‐Hang , Tan Wen‐Shan , Wong Jinnie , Ahmad Noor Azlinda , Wooi Chin‐Leong , Wu Yuan‐Kang , Ariffin Aznan Ezraie 
Published: May 2021

AbstractPeer‐to‐peer (P2P) energy trading is expected to be emerged as a new energy management paradigm that allows the transaction of energy from one prosumer to another prosumer without any dependency on a central controller. In Malaysia, first pilot test of P2P trading has been conducted recently, which exclusively participated by industrial prosumers and commercial consumers only. In order to investigate the impact of P2P energy trading to Malaysia market, this paper proposes an auction‐based two‐stage P2P trading market‐clearing strategy, for multi‐cities and intra‐city in Malaysia. A motivational game theory‐based price scheme is presented to ensure an unbiased market operation, to maximize the saving and payoff for each prosumer, at the same time benefits the power utility company. The proposed two‐stage market clearing model is solved by Linear Programming optimization approach. A realistic representation of P2P trading in Malaysia is constructed and tested out under multi‐cities and intra‐city energy trading test cases. Comparison between the proposed P2P energy trading with existing solar generation net metering scheme is also presented. The numerical results indicate the viability and potential of motivational game theory based P2P trading in future Malaysia transactive energy market.
CT and pathological features in renal parenchymal malakoplakia: a case report and review of the literature
Authors: Yang Yuanfen 
Published: May 2021

AbstractRenal malakoplakia is a rare form of chronic inflammatory granulomatous disease in the kidney. It occurs in adult patients with immunocompromised status or debilitating disease. In the present study, we reported a case of a 50-year-old woman with no underlying disease. This report describes the CT and pathological features of renal malakoplakia in a 50-year-old woman. Plain CT scan showed a large soft tissue mass at the middle and upper pole of the left kidney. Enhanced CT scan showed delayed enhancement in the solid part of the mass. Our results might provide some useful information for the diagnosis of renal parenchymal malakoplakia.
Concerns about Data Integrity of 22 Randomized Controlled Trials in Women's Health
Authors: Liu Yizhen , Thornton Jim G. , Li Wentao , van Wely Madelon , Mol Ben W. 
Published: May 2021

Objective During a review on postpartum hemorrhage, we identified randomized controlled trials (RCTs) of one author conducted at the same time and place for the same condition, with large differences in baseline characteristics. We assessed the data integrity of the RCTs of this author.
Study Design We undertook a focused analysis of the data integrity of all RCTs published by Dr. Ahmed M. Maged. We examined the studies for clinical logic and made pairwise comparisons of baseline characteristics and outcomes between trials. We used mathematical methods to assess whether the distribution of baseline characteristics was compatible with chance.
Results Between March 2015 and December 2019, Dr. Maged published 22 RCTs (n = 3,722). The median number of participants randomized per center per month was 32 (range = 1–89). Fifteen studies were either not or retrospectively registered, with one study registered 1 year after publication. One study was submitted for publication prior to the completion of the described study period. There were many unusual findings in the studies, including biologically implausible occurrences such as the absence of an association between gestational age and birthweight in seven studies and very different body mass index between three trials, which ran at the same time in the same hospital on the same topic as well as unlikely occurrences such as limited participant drop outs. One paper contained considerable text duplication and identical data to that in a paper published by a different author group from a different hospital, with both papers submitted at the same time. Mathematical analysis of the baseline characteristics of all 22 trials indicated that at least some of the reported baseline characteristics were unlikely to be the result of proper randomization.
Conclusion Our analyses of the 22 RCTs of Dr. Maged suggest potential data integrity issues in at least some of them. We suggest that journals investigate according to the Committee on Publication Ethics guidelines. The procedures demonstrated in this paper may help to assess data integrity in future attempts to verify the authenticity of published RCTs.
Key Points
Gender roles produce divergent economic expectations
Authors: D’Acunto Francesco , Malmendier Ulrike , Weber Michael 
Published: May 2021

Significance
Women and men expect systematically different levels of future inflation. This gender expectations gap can be detrimental for women’s economic choices and long-term wealth, because it might reduce the effectiveness of economic policies, induce stress, and affect women’s well being. Using data for a representative US population, we document that traditional gender roles, rather than innate characteristics, shape the gender expectations gap. By doing most of the grocery shopping for their households, women observe different price signals than men. Because grocery prices are volatile and positive price changes are especially memorable, women end up expecting systematically higher inflation than men. The gender expectations gap disappears if grocery chores are distributed equally within households.