bluuebunny/crossref_metadata_2025_split huggingface dataset:
Frozen Vein Wrapping for Chronic Nerve Constriction Injury Reduces Sciatic Nerve Allodynia in a Rat Model
Authors: Mukai Michiaki , Uchida Kentaro , Hirosawa Naoya , Murakami Kenichi , Inoue Gen , Miyagi Masayuki , Shiga Yasuhiro , Inage Kazuhide , Sekiguchi Hiroyuki , Orita Sumihisa , Suzuki Takane , Matsuura Yusuke , Takaso Masashi , Ohtori Seiji 
Published: June 2021

Abstract
BackgroundAutologous vein wrapping (VW) is used in the treatment of recurrent chronic constriction neuropathy and traumatic peripheral nerve injury. However, use of autologous veins is limited by the inability to obtain longer veins of sufficient length for larger sites. Frozen allograft tissue has several advantages, including its availability for large grafts, avoidance of donor-site morbidity, and shorter operation time. Here, we investigated the effect of frozen vein wrapping (FVW) in Wistar rats as a model of sciatic nerve injury. MethodsThe rats were grouped by treatment as (i) untreated after chronic constriction injury surgery (CCI; control group), (ii) treated with vein wrapping using freshly isolated vein (VW), and (iii) treated with vein wrapping using frozen vein (FVW). Mechanical allodynia was assessed with von Frey filaments on postoperative days (PODs) 1, 3, 5, 7, and 14. ResultsThe response of heme oxygenase-1 gene, Hmox-1, expression to VW and FVW was assessed by RT-PCR. Both VW and FVW significantly increased withdrawal threshold levels compared to the untreated control group on POD 1, 3, and 5. Both VW and FVW also showed increased HO-1 expression compared to the CCI group. ConclusionsOur results suggest that FVW may be a suitable therapeutic optionas a source of large grafts.
The Quantity of G-Quadruplex in Bacterial Genome affects G-Quadruplex Ligand Sensitivity in Hypertension associated Tongue Coating Microbiota
Authors: Zhang Shiqin , Liu Yangong , Zhou Jiang , Cui Qinghua , Chen Feng , Xu Ming 
Published: June 2021

Abstract
Background Recently, more and more attention has been paid to the role of oral microbiota in hypertension. It was reported that the disorder of tongue coating microbiota would significantly increase systolic blood pressure, yet the characteristics of tongue coating microbiota of hypertensives remain unknown. Microbiota is regulated by genes, G-quadruplex (G4) is a secondary structure of nucleic acid, which plays an important role in the regulation of microbial biologic features. there are many G4 ligands in oral administration, but how G4 ligands affect the bacterial biological phenotype needs further exploration. Results We used metagenomics for analysis of 58 subjects including 23 healthy subjects and 35 hypertensives, and bioinformatics technology for detecting G4 characteristic sequences, finally verified by biological and chemical experiments. We found that Actinomyces decreased significantly in the hypertension group with the highest average maximum putative G-quadruplex forming sequences (PQS) and GC quantity. We also screened out two species with significantly different abundance between two groups, Actinomyces odontolyticus and Acinetobacter baumannii. A. odontolyticus had higher GC% and frequency of PQS per 1000bp in the genome, which led to differential inhibition of bacterial growth, metabolism, biofilm formation by G4 ligand, sanguinarine. Major Facilitator Superfamily (MFS) was found to be involved in these biological phenomena, we found that sanguinarine could bind and stabilize the G4 structure related to MFS and further inhibited the expression of MFS.ConclusionsDifferent quantities of G4 sequences in the bacterial genome affect G4 ligand sensitivity in hypertension-associated tongue coating species, A. odontolyticus and A. baumannii. G4 ligand (sanguinarine) can bind and stabilize the G4 characteristic sequence of the MFS gene to inhibit the expression, and then inhibit the growth, biofilm formation and metabolism. This study provides a theoretical basis for the selection of G4 based drugs in the oral cavity.
The Association of Sleep Duration with Visual Impairment in Middle-aged and Elderly Adults: The China Health and Retirement Longitudinal Baseline
Authors: Sun Mengsha , Bo Qiyu , Lu Bing , Sun Xiaodong , zhou minwen 
Published: June 2021

Abstract
Background

Sleep disorders may heighten the risk of visual impairment to further impact health outcomes. Little is known regarding the association of visual impairment with sleep disorders in China. Our objective was to examine the association of visual impairment with sleep disorders.
Methods

This cross-sectional study used the data from 13264 respondents to the 2011 survey of the China Health and Retirement Longitudinal Study, a nationally representative survey of adults aged 45 years or older. Visual impairment (VI) and sleep duration were examined using self-reported questionnaires. Respondents were identified as having VI if they reported blindness or partial blindness. With regards to sleep duration, participants were categorized into three groups: 1) those reporting short sleep duration (≤ 6 hours/night), 2) those reporting long sleep duration (> 8 hours/night), and 3) those reporting 6 to 8 hours of sleep per night (used as the reference group). Weighted multilevel logistic regression models, adjusting for sociodemographic characteristics, health behaviors, and medical history, were used.
Results

Of 13,264 respondents, 6,880 (51.9%) were women. The mean, standard deviation (SD) age was 59.39 (9.71) years. A total of 842 (6.3%) of respondents reported VI. The prevalence of short and long sleep duration was significantly higher among respondents with VI than those without VI (P < 0.001). The associations also persisted after stratifying the sample by age or sex. Multilevel logistic regression models showed that compared with 6–8 h/night of sleep, sleep duration of ≤ 6h/night was associated with a 1.19-fold (95% confidence interval (CI) = 1.02–1.40) higher VI risk, and sleep duration of > 8 h/night was associated with a 1.36-fold (95% CI = 1.05–1.75) higher VI risk. Higher risk of VI was associated with short (odds ratio [OR] = 1.34, 95% CI: 1.04–1.73) and long (OR = 1.60, 95% CI: 1.04–2.44) sleep durations in middle-aged respondents, as well as short sleep duration (OR = 1.27, 95% CI: 1.05–1.55) in elderly respondents. However, the association between VI and long sleep duration (OR = 1.34, 95% CI = 0.97–1.84) was absent in elderly respondents.
Conclusion

In this study, both short and long sleep durations were associated with VI. More comprehensive and integrated health care and rehabilitation systems covering vision and sleep are needed to address age-related VI.
Optimization of Culture Conditions in a Bioreactor for the Production of Biomass and Metabolites of the Macromycete Lentinula Edodes
Authors: Chegwin-Angarita Carolina , Bello-Forero Robinson A. , Serrato-Bermúdez Juan C. 
Published: June 2021

Abstract
Lentinula edodes is an edible mushroom known for its medicinal properties attributed to different secondary metabolites. In this article, the effect of glucose concentration, aeration and agitation on the production of biomass and intrametabolites in submerged fermentation was evaluated. By means of a Box-Behnken design, these variables were optimized to obtain a multifunctional biomass. It was determined that both glucose concentration and agitation had a significant effect on the response variables (biomass, sterols and polysaccharides). Under the evaluated conditions, the values of the variables that optimize biomass production were 1.2 vvm, 60 rpm and 21.97 g / L of glucose. Subsequently, the sterols and total carbohydrates extracted from the biomass were quantified through a spectrophotometric analysis. To optimize these two response variables, similar values are required for aeration (1.2 vvm) and agitation (60 rpm), while the glucose concentration was 16.32 g / L and 19.6 g / L for sterols and total polysaccharides respectively.
New Modeling of AgFeNi2S4-Graphene-TiO2 Ternary Nanocomposite Synthesized Via the Pechini Method for the Enhanced Electrophotocatalytic Reduction of CO2
Authors: Otgonbayar Zambaga , Oh Won-Chun 
Published: June 2021

Abstract
For reduction of CO2 into hydrocarbon fuels, an AgFeNi2S4-Graphene-TiO2 ternary nanocomposite material was synthesized via the Pechini method. The Pechini method is based on a chelating agent which, together with ethylene glycol (C2H6O2) and citric acid (C6H8O7) as a chelating cation, can affect the structure and stability of the nanocomposite. The catalytic activity of the photocatalyst for photocatalytic and electrochemical CO2 evolution into hydrocarbon fuels was tested. The methanol yield under UV light was 8.679 %, 6.349 %, and 4.136 %, and the methanol yields under visible light was 6.291 %, 4.738 %, and 2.339 %, respectively. The stability and reusability of the photocatalyst remained high after a 4-cycle recycling test without a decrease in yield of the final photocatalytic CO2 reduction product. The enhanced photoreduction of CO2 through the as-prepared ternary photocatalyst can be ascribed to the catalyst's conformation and low recombination rate. In electrochemical CO2 reduction, the Faraday efficiency is the main parameter that defines the performance of the working electrode and the evolution of methanol. The Faraday efficiency of AFNSGT ternary nanocomposite was 44.25 %; this is an increase in the value of the Faraday efficiency, which proves that the design of the new nanocomposite successfully increases the activity of the working electrode and has a positive effect on the electrochemical reduction of CO2. The photocatalytic and electrochemical CO2 reduction data show that the preparation method, morphological state, and charge carrier properties of the photocatalyst are important for the catalytic activity and efficiency of the methanol evolution pathway. This study provides a strategy for fabrication of a new ternary nanocomposite based on 2D-structured graphene, TiO2, and a quaternary nanocomposite.
Effect of SiCw Whiskers on the indentation thermal shock resistance of ZrB2-MoSi2 Composites
Authors: Paul Tanay Rudra , Mondal Manas Kumar , Mallik Manab 
Published: June 2021

Abstract
A comparative evaluation of the thermal shock resistance (TSR) of ZrB2-20 MoSi2-20 SiCw (ZMSw20) and ZrB2-20 MoSi2 − 5SiCw (ZMSw5) composites were studied using the indentation quench method. High-dense ZrB2 based composites were prepared by multi-stage spark plasma sintering at 1700°C. The results show that the ZMSw20 composite expressed superior crack shielding and TSR under quenching circumstances. The critical temperature differential ΔTc of ZMSw20 ceramic (ΔTc= 800°C) was higher than that of ZMSw5 ceramic (ΔTc=600°C). The significant enhancement in TSR was imposed residual stresses that improved the resistance to crack progression during thermal shock. Furthermore, an increment in silicon carbide content reduces the crack growth, and increases the TSR of the composites.
Thermal Conductive Networks Constructed by Sialon Fibers in-Situ Synthesized in Barium Aluminosilicate Glass-Ceramic
Authors: Sun Shaojie , Wang Xinyu , Zhou Junjie , Zhang Siqi , Ge Kongyu , Yang Hua , Zhang Tao , Xiong Li , Qin Chunlin , Wen Guangwu , Xia Long 
Published: June 2021

Abstract
The application of ceramic materials is limited due to the complicated preparation process and intrinsic brittleness. In this work, a pressureless manufacturing route that enables the formation of barium aluminosilicate (BAS) glass-ceramic consisting of internal β-Sialon fibers with enhanced thermal conductivity is developed. By adjusting the carbon source content, composites with different Sialon contents can be easily fabricated. The thermal conductivity of the sample with 3.5 wt.% is improved to 5.845 W/m ∙ K with the Sialon content of 26 wt.% in the composite, which is 112.64 % higher than that of the pure BAS matrix. The theoretical models suggest that the enhanced thermal conductivity is mainly ascribed to the thermal conduction network constructed by Sialon fibers. This work provides a method with industrial application prosperity to fabricate the high temperature ceramic matrix composite of different sizes and complex shapes.
Spectral Matching of Three-Component Seismic Ground Accelerations for Nuclear Power Plants
Authors: Mangkoesoebroto Sindur , Zulkifli Ediansjah , Yasa Adi P. 
Published: June 2021

Abstract
The aim of the paper is to introduce a new procedure of three-component spectral matching of seismic ground acceleration records. The procedure is straightforward, yet it is general. In principle, the procedure involves varying of both the Fourier amplitude and the phase spectra so that the modified records’ spectra agree with a target. The matching can be performed against either a target Fourier or response spectra. In the former the solution is exact, while in the latter it becomes approximate. A target spectrum representative of three directions should be provided. In the example several three-component records were matched against two target spectra. Good convergence was achieved in velocity and displacement records so that no baseline correction was necessary. The couplings among the components were preserved.
Mapping Research Evidence on Children With Atopic Dermatitis and Quality of Life in Sub-saharan Africa: a Scoping Review Protocol
Authors: Kelbore Abraham Getachew , Enbiale Wendemagegn , Mosam Anisa , Wyk Jacqueline M. van 
Published: June 2021

Abstract
Background: Atopic dermatitis (AD) is a chronic, debilitating disease affecting children worldwide. Several studies have explored the disease causes a significant problem leading to a diminished quality of life, for the affected children but systematic evaluation of such studies in Africa is yet to be reported. Therefore, this scoping review aims to map research evidence on children with AD and their quality of life (QoL) in sub-Saharan Africa.Methods: The scoping review will follow the Arksey and O’Mally methodological framework. The electronic databases to be searched will include: PubMed, EBSCOhost (Academic search complete, CINAHL PsycINFO, and Health Sources), Scopus, and Google Scholar for published literature between 2000 and 2021. Only literature written in English will be included. The search strategy for the databases will include keywords, Medical Subject Headings terms and Boolean operators. The reference list of the included sources of evidence, and the WHO website will also be consulted for evidence relating to QoL of children with AD in Sab-Saharan Africa (SSA). Following title searching, two independent reviewers will conduct screening of abstracts and full text articles. Eligibility criteria will guide the screenings. This review will include studies conducted in SSA, publication focusing on quality of life and associated factors of AD in children. Data will be extracted from the included studies, analyzed qualitatively; NVIVO software V.11 will be usedand the emerging themes reported narratively. The mixed-method appraisal tool (MMAT) will be employed for quality appraisal of included studies.Discussion: We look forward to findings of several studies that describe the QoL and associated factors among children with AD and that report on using different diagnostic criteria, severity scaling and QoL measuring scale tools used to ascertain the presence of AD, scale severity of AD and impact of AD on quality of life among children. The study findings will be disseminated through publication in a peer-reviewed journal, peer presentations, and presentations at relevant conferences.
Prognostic Value of Systemic Inflammatory Markers and the Nutrition Status in Thymic Epithelial Tumors with Complete Resection
Authors: Sakane Tadashi , Okuda Katsuhiro , Matsui Takuya , Oda Risa , Tatematsu Tsutomu , Yokota Keisuke , Nakanishi Ryoichi 
Published: June 2021

Abstract
BackgroundRecent studies have shown that several systemic inflammatory markers and the nutrition status, including the neutrophil-to-lymphocyte ratio (NLR), monocyte-to-lymphocyte ratio (MLR), platelet-to-lymphocyte ratio (PLR), and prognostic nutritional index (PNI), are useful prognostic factors in several malignant tumors. The present study explored the prognostic value of the NLR, MLR, PLR, and PNI in thymic epithelial tumor (TET) patients who underwent complete resection.MethodsA total of 158 TET patients who underwent complete resection were involved in the analysis. Their NLR, MLR, PLR, and PNI values were obtained from a blood examination within one month before the initiation of treatment. A receiver operating characteristic curve analysis was conducted to determine the optimal cut-off values. ResultsThe enrolled patients were stratified by cut-offs of 4.35 for the NLR, 0.22 for the MLR, 130.18 for the PLR, and 44.02 for the PNI. A univariate analysis revealed that high-grade malignant TET, including type B2 and B3 thymoma, thymic carcinoma, and thymic neuroendocrine tumor; an advanced Masaoka stage; a high NLR; a high MLR; and a low PNI were significant predictors of a poor disease-free survival (DFS). A multivariate analysis confirmed that an advanced Masaoka stage (HR = 5.5557, P = 0.0007) and a high MLR (HR = 3.3371, P = 0.0264) were independent predictors of a poor DFS.ConclusionsOur study demonstrated that the pretreatment MLR was an independent predictor of the DFS in patients with TETs who underwent complete resection.
Differential Effects of WRAP53 Transcript Variants on the Biological Behaviours of Human Non-Small Cell Lung Cancer Cells
Authors: Zhu Yan , Sun Wenjie , Jiang Xueping , Bai Rui , Luo Yuan , Gao Yanping , Li Shuying , Huang Zhengrong , Gong Yan , Xie Conghua 
Published: June 2021

Abstract
Background: The WD40-encoding RNA antisense to p53 (WRAP53) gene, an antisense gene of TP53, has 3 different transcriptional start sites that yield 3 transcript variants. One of these variants WRAP53-1β encodes a WD repeat-containing protein WRAP53β, whereas WRAP53-1α is a noncoding RNA that regulates p53 mRNA levels. These variants are involved in the progression of non-small cell lung cancer (NSCLC). However, how the different transcript variants regulate NSCLC cell behaviours is to be elucidated.Methods: Wild-type p53 NSCLC A549 cells and p53-mutated H1975 cells were transfected with WRAP53-1α and WRAP53-1β siRNAs, and their behaviours were examined colony formation, cell viability, apoptosis, cell cycle, wound healing, and cell invasion assays.Results: WRAP53-1α knockdown increased the mRNA and protein levels of p53, whereas depletion of WRAP53-1β had no effect on p53 expression. WRAP53-1α knockdown suppressed colony formation and proliferation of A549 cells, but had the opposite effects on H1975 cells. However, WRAP53-1β knockdown promoted A549 cell growth. Depletion of WRAP53-1α and WRAP53-1β promoted apoptosis in A549 but not H1975 cells. WRAP53-1α knockdown increased the proportion of A549 but not H1975 cells at the G0/G1 phase. However, WRAP53-1β knockdown decreased the proportion of cells at the G0/G1 phase in A549 cells. Depletion of WRAP53-1α suppressed A549 cell migration and invasion, and promoted H1975 cell migration and invasion. However, depletion of WRAP53-1β had the opposite effects.Conclusions: The 2 WRAP53 transcript variants exerted opposite functions in NSCLC cells and regulated NSCLC cell behaviours in a p53-dependent manner.
RNA Sequencing of Blood in Coronary Artery Disease: Involvement of Regulatory T Cell Imbalance
Authors: McCaffrey Timothy , Toma Ian , Yang Zhaoquing , Katz Richard , Reiner Jonathan , Mazhari Ramesh , Shah Palak , Tackett Michael , Jones Dan , Jepson Tisha , Falk Zachary , Wargodsky Richard , Shtakalo Dmitry , Antonets Denis , Ertle Justin , Kim Ju H. , Lai Yinglei , Arslan Zeynep , Aledort Emily , Alfaraidy Maha , Laurent Georges St. 
Published: June 2021

Abstract
RATIONALE: Cardiovascular disease had a global prevalence of 523 million cases and 18.6 million deaths in 2019. The current standard for diagnosing coronary artery disease (CAD) is coronary angiography. Surprisingly, despite well-established clinical indications, up to 40% of the one million invasive cardiac catheterizations return a result of ‘no blockage’. OBJECTIVE: The present studies employed RNA sequencing of whole blood to identify an RNA signature in patients presenting with a clinical suspicion of CAD.METHODS AND RESULTS: Whole blood RNA was depleted of ribosomal RNA (rRNA) and analyzed by single-molecule sequencing of RNA (RNAseq) to identify transcripts associated with CAD (TRACs) in a discovery group of 96 patients presenting for elective coronary catheterization. The resulting transcript counts were compared between groups to identify differentially expressed genes (DEGs). Surprisingly, 98% of DEGs/TRACs were down-regulated ~1.7-fold in patients with mild to severe CAD (>20% stenosis). The TRACs were independent of comorbid risk factors for CAD, such as gender, hypertension, and smoking. Bioinformatic analysis identified an enrichment in transcripts such as FoxP1, ICOSLG, IKZF4/Eos, SMYD3, TRIM28, and TCF3/E2A that are likely markers of regulatory T cells (Treg), consistent with known reductions in Tregs in CAD. A validation cohort of 80 patients confirmed the overall pattern (92% down-regulation) and supported many of the Treg-related changes. TRACs were enriched for transcripts associated with stress granules, which sequester RNAs, and ciliary and synaptic transcripts, possibly consistent with changes in the immune synapse of developing T cells.CONCLUSIONS: These studies identify a novel mRNA signature of a Treg-like defect in CAD patients and provides a blueprint for a diagnostic test for CAD. The pattern of changes is consistent with stress-related changes in the maturation of T and Treg cells, possibly due to changes in the immune synapse.
Ultralong free-standing single crystal perovskite microwires with extremely low dark current
Authors: Geng Xiangshun , Feng Qi-Xin , Tian He , Chen Weijian , Wen Xiaoming , Jia Baohua , Wang Chaolun , Wu Xing , Dun Guan-Hua , Ren Jun , Deng Ningqin , Wang Fangwei , Yan Zhaoyi , Zhang Hainan , Li Cheng , Yang Yi , Xie Dan , Ren Tian-Ling 
Published: June 2021

Abstract
Super long perovskite microwires (PMWs) are in a great demand in many fields such as low-loss microcables and integrated optical waveguide. Despite decades of research into PMWs, single crystal PMWs with several centimeters long have not been obtained. Here, ultralong (up to 7.6 centimeters) monoclinic crystal structure CH3NH3PbI3·DMF PMWs have been synthesized. The high-quality microwire exhibits long carrier lifetime of 1775.7 ns. The as-prepared free-standing PMWs can be integrated to any arbitrary substrate and 808 nm near-infrared photodetectors have been successfully demonstrated. The fabricated device shows a high light on/off ratio of 1.79×106 and an extremely low dark current of 2.5 fA at 1 V bias. This work provides a strategy for the solution growth of ultralong microwires.
A review: controversies in blood component use in newborns
Authors: Luban N.L.C. 
Published: June 2021

Abstract
Advances in the care of the critically ill neonate have necessitated dramatic changes in the blood bank’s response, especially to the needs of the low-weight premature infant. The neonate is now considered a major consumer of blood products, and the packaging and administration of these products must be altered to suit its specialized needs. The indications and preparations for small-volume red blood cell (RBC) transfusions are of particular concern. Smallvolume RBC transfusions are both the most frequently used and the most controversial in the treatment of neonates. A review and guidelines for the use of RBCs for the perinatal patient are presented.
Immunobematology
1991; 1:1–7.