bluuebunny/crossref_metadata_2025_split huggingface dataset:
Chemoproteomics of microbiota metabolites reveals small-molecule agonists for orphan receptor GPRC5A
Authors: Zhao Xiaohui , Stein Kathryn R. , Chen Victor , Griffin Matthew E. , Hang Howard C. 
Published: December 2021

ABSTRACTThe microbiota generates diverse metabolites that can engage multiple pathways to modulate host physiology and disease, but their protein targets and mechanism(s) of action have not been fully elucidated. To address this challenge, we focused on indole-3-acetic acid (IAA), a prominent microbiota metabolite, and developed IAA-based chemical reporters for proteomic studies. We discovered that IAA interacts with many proteins in host cells, including small-molecule transporters, receptors and metabolic enzymes. Notably, our functional studies revealed that IAA binds to orphan G protein-coupled receptors such as GPRC5A, but only aromatic monoamines were capable of inducing GPRC5A signaling. Functional profiling of microbiota uncovered specific bacterial species and enzymes that generate GPRC5A agonists. Finally, biochemical characterization of GPRC5A activation identified more potent synthetic agonists as well as key amino acid residues involved in ligand binding. These studies highlight the utility of chemoproteomics to dissect protein targets and mechanisms of action for microbiota metabolites.
Genomic epidemiological models describe pathogen evolution across fitness valleys
Authors: Cárdenas Pablo , Corredor Vladimir , Santos-Vega Mauricio 
Published: December 2021

AbstractGenomics is fundamentally changing epidemiological research. However, systematically exploring hypotheses in pathogen evolution requires new modeling tools. Models intertwining pathogen epidemiology and genomic evolution can help understand processes such as the emergence of novel pathogen genotypes with higher transmissibility or resistance to treatment. In this work, we present Opqua, a flexible simulation framework that explicitly links epidemiology to sequence evolution and selection. We use Opqua to study determinants of evolution across fitness valleys. We confirm that competition can limit evolution in high transmission environments and find that low transmission, host mobility, and complex pathogen life cycles facilitate reaching new adaptive peaks through population bottlenecks and decoupling of selective pressures. The results show the potential of genomic epidemiological modeling as a tool in infectious disease research.
Evaluation of a phenotypic, point-of-care solution for the detection and quantitative antibiotic susceptibility testing for lower Urinary Tract Infection
Authors: Turner R. , Kirkby R. , Meader E. , Wain J. 
Published: December 2021

AbstractBackgroundUrinary tract infections (UTIs) are one of the most common bacterial infections seen in primary care. The current standard for diagnosis is microbiological culture and antibiotic sensitivity testing of a mid-stream urine sample; however, this technique is costly, labour intensive and typically takes 2-3 days to yield a result.Study design and ObjectiveThis is a nonexperimental cross-sectional study. The aim of this study was to evaluate the efficacy of U-treat, a bioluminescent approach for rapid detection of bacteriuria and quantitative determination of the antimicrobial susceptibility profiles of uropathogens in clinical urine specimens - in under an hour.MethodThe evaluation was carried out in two UK-based Medical Centres using urine samples from patients presenting with symptoms of a UTI (n=249). The U-treat technology is a two test, two reagent process. Test 1 detects the presence of a bacterial UTI > 104 bacteria/mL (5-10 minutes). Test 2 produces quantitative antibiotic susceptibility (<50 minutes). Only urine samples testing positive for bacteria in Test 1 underwent Test 2 (n=82). U-treat results were compared retrospectively against reference laboratory culture and sensitivity findings. The influence of the technology on patient treatment outcomes was also analysed.ResultsRelative to reference laboratory analysis, Test 1 showed a sensitivity of 97.1% and specificity of 92.0%. (PPV: 89.3%; NPV: 97.8%). Test 2 produced an overall sensitivity (measurement of true susceptibility) of 94.1% (Predictive value: 96%) and an overall specificity (measurement of true resistance) of 90.5% (Predictive value 86.4%). Analysis of treatment data demonstrated that had the physicians had access to U-treat results at the point of care, the percentage of patients treated successfully would have risen from 68.3% to 92.7%.ConclusionU-treat represents the first technology, world-wide, capable of providing UTI treatment data to physicians at the point of care, in less than 60 minutes.
Matrix metalloproteinase 2 and 9 enzymatic activities are selectively increased in the myocardium of Chronic Chagas Disease cardiomyopathy patients: role of TIMPs
Authors: Baron Monique Andrade , Ferreira Ludmila Rodrigues Pinto , Teixeira Priscila Camillo , Moretti Ana Iochabel Soares , Santos Ronaldo Honorato Barros , Frade Amanda Farage , Kuramoto Andréia , Debbas Victor , Benvenuti Luiz Alberto , Gaiotto Fabio Antônio , Bacal Fernando , Pomerantzeff Pablo , Chevillard Christophe , Kalil Jorge , Cunha-Neto Edecio 
Published: December 2021

AbstractChronic Chagas disease (CCC) is an inflammatory dilated cardiomyopathy with a worse prognosis compared to other cardiomyopathies. We show the expression and activity of Matrix Metalloproteinases (MMP) and of their inhibitors TIMP (tissue inhibitor of metalloproteinases) in myocardial samples of end stage CCC, idiopathic dilated cardiomyopathy (DCM) patients, and from organ donors. Our results showed significantly increased mRNA expression of several MMPs, several TIMPs and EMMPRIN in CCC and DCM samples. MMP-2 and TIMP-2 protein levels were significantly elevated in both sample groups, while MMP-9 protein level was exclusively increased in CCC. MMPs 2 and 9 activities were also exclusively increased in CCC. Results suggest that the balance between proteins that inhibit the MMP-2 and 9 is shifted toward their activation. Inflammation-induced increases in MMP-2 and 9 activity and expression associated with imbalanced TIMP regulation could be related to a more extensive heart remodeling and poorer prognosis in CCC patients.
A dual reporter system identifies an intermediate state and sequential regulators of 2-cell-like-to-pluripotent state transition
Authors: Zhang Chao , Hao Jing , Shi Ming , Li Yu-Xuan , Yao Wang , Wang Yangming 
Published: December 2021

SummaryMouse embryonic stem cells (ESCs) cycle in and out of 2-cell-like (2C-like) state in culture. The molecular mechanism governing the exit of 2C-like state remains obscure, partly due to the lack of a reporter system that can genetically mark intermediate states during exiting process. Here, we identify an intermediate state that is marked by the co-expression ofMERVL::tdTomato andOCT4-GFP (MERLOT) during2C-like-to-pluripotent state transition (2CLPT). Transcriptome and epigenome analyses demonstrate that MERLOT cells cluster closely with 8-16 cell stage mouse embryos, suggesting that 2CLPT partly mimics early preimplantation development. Through a CRISPRa screen, we identify an ARRDC3-NEDD4-OCT4 regulatory axis that plays an essential role in controlling 2CLPT. Furthermore, re-evaluating previously reported 2C-like state regulators reveals dual function of Chaf1a in regulating the entry and exit of 2C-like state. Finally, ATAC-Seq footprinting analysis uncovers Klf3 as an essential transcription factor required for efficient 2CLPT. Together, our study identifies a genetically traceable intermediate state during 2CLPT and provides a valuable tool to study molecular mechanisms regulating this process.
CEO beauty and management guidance
Authors: Guo Jun , Kim Jung Yeun , Kim Sungsoo , Zhou Nan 
Published: December 2021

PurposeThe authors study whether CEO beauty influences management guidance.Design/methodology/approachThe authors calculate an attractiveness score based on facial symmetry and perform regression analyses to examine the relation between CEO beauty and management guidance.FindingsThe authors find that attractive CEOs are more likely to issue voluntary management earnings guidance. After controlling for this appearance-based self-selection, the authors document that management forecasts provided by attractive CEOs are more optimistic yet less precise. Consistent with this result, the authors find that analysts' consensus forecast error following management forecasts made by attractive CEOs is larger than such error following management forecasts made by unattractive CEOs. The authors further find that the perceived credibility of management forecasts by attractive CEOs is not different from that by unattractive CEOs.Originality/valueThese findings suggest that attractive CEOs are more active but less skillful in issuing management forecasts. This adds to the emerging accounting literature on the relation between facial appearance and information delivery.
The effect of porosity and number of unit cell on applicability of volume average approach in closed cell porous media
Authors: Wang Chunyang , Zheng Xinghua , Zhang Ting , Chen Haisheng , Mobedi Moghtada 
Published: December 2021

Purpose
The purpose of this study is to investigate the applicability of volume average which is extensively used for analyzing the heat and fluid flow (both for single-phase and solid/liquid-phase change) in a closed cell porous medium numerically.

Design/methodology/approach
Heat conduction equations for the solid frame and fluid (or phase change material) are solved for pore scale and volume average approaches. The study mainly focuses on the effect of porosity and the number of porous media unit cell on the agreement between the results of the pore scale and volume average approaches.

Findings
It is observed for the lowest porosity values such as 0.3 and the number of porous media unit cell as 4 in heat transfer direction, the results between two approaches may be questionable for the single-phase fluid. By increasing the number of porous media unit cell in heat transfer direction, the agreement between two approaches becomes better. In general, for high porosity values (such as 0.9) the agreement between the results of two approaches is in the acceptable range both for single-phase and solid/liquid-phase change. Two charts on the applicability of volume average method for single-phase and solid/liquid-phase change are presented.

Originality/value
The authors’ literature survey shows that it is the first time the applicability of volume average which is extensively used for analyzing the heat and fluid flow in a closed cell porous medium is investigated numerically.
Residents’ perceptions of environmental certification, environmental impacts and support for the world expo 2015: the moderating effect of place attachment
Authors: Prayag Girish , Chowdhury Mesbahuddin , Prajogo Daniel , Mariani Marcello , Guizzardi Andrea 
Published: December 2021

PurposeBased on social exchange theory (SET) and signaling theory (ST), this study aims to evaluate how an event’s perceived environmental certification (PEC) by residents, affect their evaluations of environmental impacts and subsequent event support (ES). The moderating role of place attachment (PA) on some of these relationships is also evaluated.Design/methodology/approachUsing partial least square structural equation modeling (PLS-SEM), a theoretical model is tested on a sample of 450 residents who attended the 2015 Milan World Expo.FindingsPEC positively affects evaluations of positive environmental impacts (PEI) but negatively affects evaluations of negative environmental impacts (NEI). PEC positively affects ES while the relationship between PEC and NEI is moderated by PA.Research limitations/implicationsItems used to measure PEC, PEI and NEI are not exhaustive. SET has its own limitations in explaining residents’ ES, which the authors have attempted to attenuate by using ST.Practical implicationsUsing environmental certification as a communication tool must demonstrate to residents how it reduces negative externalities, rather than focusing only on its positive community benefits. Less well-educated residents had the lowest ES, suggesting the need to use social media to increase ES.Originality/valueThis study contributes to understandings of the perceptions of the benefits of event certification by residents, and how this affects their ES. PA moderates the relationship between PEC and NEI.
Effects of emotional healing on organisational learning and radical innovation: a leadership-based approach
Authors: Domínguez-Escrig Emilio , Mallén-Broch Francisco Fermín , Chiva Ricardo , Lapiedra Alcamí Rafael 
Published: December 2021

PurposeThe objective of this study was to provide empirical evidence of the relationship between emotional healing, analysed as a leader behaviour and radical innovation.Design/methodology/approachTo test the hypotheses, structural equations were used, with organisational learning capability as an explanatory variable. The study was conducted in a population of 402 Spanish companies. A sample frame of 292 questionnaires was obtained from 146 different organisations. The fieldwork took place in two stages, in 2010 and again in 2015.FindingsResults confirm the hypotheses proposed in the model. Emotional healing promotes organisational learning capability and, in turn, radical innovation.Practical implicationsOur results provide some guidelines for companies that are trying to develop radical innovations by improving the workplace conditions.Originality/valueTo our knowledge, there are no previous studies which studied the relationship between emotional healing and its effect on radical innovation. Therefore, providing empirical evidence of such a relationship is the main contribution of this paper.
Supreme audit institutions in Europe: synergies, institutional transparency, gender equality and sustainability engagement
Authors: Hancu-Budui Andreea , Zorio-Grima Ana 
Published: December 2021

PurposeSupreme audit institutions (SAIs) examine and supervise the activity of public institutions. The study aims at contributing to the existing literature on public sector audit by providing a classification of 29 European SAIs – 28 national SAIs and the European Court of Auditors (ECA) – based on a broad range of attributes varying from the SAIs' environment to its structure, activity, resources or transparency.Design/methodology/approachThe authors apply quantitative methodology for clustering by means of multidimensional scaling and regressive ordinary least square (OLS) and logistic models.FindingsThe authors' results show that SAIs from veteran EU member states (MSs) are more similar amongst them and the same applies to SAIs from Nordic countries, Baltic countries, Western Mediterranean countries and Eastern countries. The authors also perform additional analysis focussing on currently relevant issues such as gender equality, age, environment or the sustainable development goals (SDGs), concluding that the younger the institutions' staff, the more transparent the institutions are. The authors also find that more transparent SAIs report on environmental audits, more prone to cover the SDGs in their audits.Research limitations/implicationsThe research is limited in purpose and scope because data cover only Europe. Given the limited number of observations (29), it does not have prospective purposes but only explanatory ones. The authors' findings are interesting for researchers because they offer original insights on public audit in Europe and cover matters of current interest such as environment, transparency or gender equality.Practical implicationsThe research is also of interest for public auditors because it offers them information that may help them improve their activity and find institutional synergies, as the dataset is available to public auditors.Social implicationsFrom a social view point, the paper shows that public auditors perform work on topics of interest for the citizens.Originality/valueThe dataset compiled for the research offers extensive data and a wide variety of attributes defining European SAIs and may offer future opportunities for research from different perspectives.
Who is likely to hide knowledge after peer ostracism? An exchange-based perspective of contact quality and need to belong
Authors: Fatima Tehreem , Bilal Ahmad Raza , Imran Muhammad Kashif , Ayub Arslan , Arshad Hira 
Published: December 2021

Purpose
The purpose of this study is to uncover how peer ostracism (POS) elicits knowledge hiding directed towards ostracizing peers through the intervening role of peer contact quality (PCQ). Moreover, the authors aim to highlight the role of the need to belong (NTB) as a first-order boundary condition in direct and indirect hypothesized paths.

Design/methodology/approach
The research opted for a three-wave time-lagged survey design. The data were obtained from the 234 teaching and non-teaching employees working in Higher Educational Sector in Pakistan through random sampling. Mediation and moderated mediation analysis was done by using PROCESS Models 4 and 7.

Findings
The results embraced the mediation, moderation and moderated mediation hypotheses. It was noted that POS creates negative exchange relationships. As a result, the ostracized employees withhold knowledge from the predating peer. NTB served as a buffering agent between POS and PCQ, as well as, in the indirect POS, PCQ and peer-directed knowledge hiding relationship.

Practical implications
This research serves as a guideline for management and faculty of Higher Educational Institutions for minimization of POS to promote effective collegial contact quality and curb knowledge hiding.

Originality/value
Although the research in workplace ostracism and knowledge hiding is not new, yet how this association emerges from the viewpoint of peers is not known. This study has added to the literature by answering who is more likely to reciprocate ostracism from peers by having poor quality contact and directing knowledge hiding towards the predator. By this, the authors have added to the limited stream of moderated mediation mechanisms underlying ostracism and knowledge hiding behaviour. In addition, the authors have drawn attention to the importance of peer relationships in higher educational settings.
Stroke survivors' perspectives on recovering in rural and remote Australia: A systematic mixed studies review
Authors: Jackson Sarah M. , Neibling Bridee A. , Barker Ruth N. 
Published: December 2021

AbstractIntroductionStroke survivors recovering in rural and remote locations often have little or no access to rehabilitation services. The purpose of this study was to review the literature on recovering in rural and remote Australia, from the perspective of stroke survivors. Use of technology to support recovery was also explored.MethodsA systematic mixed studies review was conducted and reported according to the ENTREQ and PRISMA statements. MEDLINE (Ovid), CINAHL (EBSCOhost), Scopus, PsycINFO (ProQuest), Cochrane Library and Google Scholar were searched from inception to May 2021 for studies investigating stroke survivors' perspectives on recovering in rural or remote Australia. Qualitative, quantitative or mixed methods studies were included. Methodological quality was assessed using the Mixed Methods Appraisal Tool. Studies were not excluded or weighted according to methodological quality. To review the perspectives of stroke survivors on recovering, findings of included studies were mapped to the Living My Life framework and integrated using a convergent qualitative synthesis. The review protocol was registered on PROSPERO (CRD42017064990).ResultsEight studies met the inclusion criteria: six qualitative, one quantitative and one mixed methods. Methodological quality of the small number of studies ranged from low to high, indicating further high‐quality research is needed. Included studies involved 152 stroke survivors in total. Review findings indicated that recovering was driven by working towards what mattered to stroke survivors, in ways that matched their beliefs and preferences and that worked for them in their world, including use of technology.ConclusionStroke survivors recovering in rural and remote locations want to live their life by doing what matters to them, despite the challenges they face. They want support in ways that work for them in their environment. Further research is required to tailor support for stroke survivors that is specific to their life in rural and remote locations.
Tensions between (Post)Bureaucratic and Neo‐normative Demands: Investigating Employees’ Subjective Positions at EurAirport
Authors: Bardon Thibaut , Josserand Emmanuel , Sferrazzo Roberta , Clegg Stewart 
Published: December 2021

AbstractThe introduction of a neo‐normative discourse in a (post)bureaucratic organization can result in tensions between the neo‐normative injunction to be authentic and exhortations to fit with the ideal (post)bureaucratic organizational subject. Focusing on how shopfloor workers subjectively experience the tensions between neo‐normative and (post)bureaucratic demands, this empirical investigation yielded three major contributions. First, it pinpointed and addressed significant gaps in existing studies of normative and neo‐normative discourse. Second, the study better distinguished normative and neo‐normative control on the basis of two tensions: (1) authenticity versus conformity; and (2) conflation versus differentiation between life and work. Third, the study identified four distinct subject positions that demonstrated how organizational participants creatively appropriate and strive to resolve these two tensions in a work setting that mixes normative and neo‐normative control.
Predictive performance for placental dysfunction related stillbirth of the competing risks model for small‐for‐gestational‐age fetuses
Authors: Nicolaides Kypros H. , Papastefanou Ioannis , Syngelaki Argyro , Ashoor Ghalia , Akolekar Ranjit 
Published: December 2021

AbstractObjectivesTo examine the predictive performance for placental dysfunction related stillbirths of the competing risks model for small‐for‐gestational‐age (SGA) fetuses based on a combination of maternal risk factors, estimated fetal weight (EFW) and uterine artery pulsatility index (UtA‐PI); and second, to compare the performance of this model with that of a stillbirth‐specific model using the same biomarkers and with the Royal College of Obstetricians and Gynecologists (RCOG) guideline for the investigation and management of the SGA fetus.DesignProspective observational study.SettingTwo UK maternity hospitals.PopulationA total of 131 514 women with singleton pregnancies attending for routine ultrasound examination at 19–24 weeks of gestation.MethodsThe predictive performance for stillbirth achieved by three models was compared.Main Outcome MeasurePlacental dysfunction related stillbirth.ResultsAt 10% false‐positive rate, the competing risks model predicted 59%, 66% and 71% of placental dysfunction related stillbirths, at any gestation, at <37 weeks and at <32 weeks, respectively, which were similar to the respective figures of 62%, 70% and 73% for the stillbirth‐specific model. At a screen positive rate of 21.8%, as defined by the RCOG guideline, the competing risks model predicted 71%, 76% and 79% of placental dysfunction related stillbirths at any gestation, at <37 weeks and at <32 weeks, respectively, and the respective figures for the RCOG guideline were 40%, 44% and 42%.ConclusionThe predictive performance for placental dysfunction related stillbirths by the competing risks model for SGA was similar to that of the stillbirth‐specific model and superior to that of the RCOG guideline.Tweetable AbstractThe competing risks approach for SGA is superior to the RCOG guideline in the prediction of placental dysfunction related stillbirths.