bluuebunny/crossref_metadata_2025_split huggingface dataset:
Improved Mechanical Strength, Proton Conductivity and Power Density in an ‘All-protonic’ Ceramic Fuel Cell at Intermediate Temperature
Authors: Azad Abul K. , Abdalla Abdalla M. , Afif Ahmed , Azad Atia , Afroze Shammya , Idris Azam Che , Park Jun-Young , Radenahmad Nikdalila , Hossain Shahzad , Zaini Juliana , Hinai Amer Al , Reza Md. Sumon , Irvine John 
Published: June 2021

Abstract
Protonic ceramic fuel cells (PCFCs) have become the most efficient, clean and cost-effective electrochemical energy conversion devices in recent years. While significant progress has been made in developing proton conducting electrolyte materials, mechanical strength and durability still need to be improved for efficient applications. We report that adding 5 mol% Zn to the Y-doped barium cerate-zirconate perovskite electrolyte material can significantly improve the sintering properties, mechanical strength, durability and performance. Using same proton conducting material in anodes, electrolytes and cathodes to make a strong structural backbone shows clear advantages in mechanical strength over other arrangements with different materials. Rietveld analysis of the X-ray and neutron diffraction data of BaCe0.7Zr0.1Y0.15Zn0.05O3-δ (BCZYZn05) revealed a pure orthorhombic structure belonging to the Pbnm space group. Structural and electrochemical analyses indicate highly dense and high proton conductivity at intermediate temperature (400-700 °C). The anode-supported single cell, NiO-BCZYZn05 | BCZYZn05 | BSCF-BCZYZn05, demonstrates a peak power density of 872 mW cm-2 at 700 °C which is one of the highest power density in an all-protonic solid oxide fuel cell. This observation represents an important step towards commercially viable SOFC technology.
TANGO - Effect of Tango Argentino on cancer-associated fatigue in breast cancer patients: study protocol for a randomized controlled trial
Authors: Oei Shiao Li , Rieser Thomas , Becker Sarah , Groß Jessica , Matthes Harald , Schad Friedemann , Thronicke Anja 
Published: June 2021

Abstract
Background The majority of breast cancer patients suffer from persistent impairments after completion of their primary oncological therapy. Cancer-related fatigue (CRF) in particular is a multidimensional syndrome having a profound negative impact on the quality of life. To counter CRF symptoms, exercise and physical activity are suggested as first-line interventions, mind-body therapies have been shown to be effective, and music therapy can also reduce anxiety and stress in breast cancer patients. Tango therapy that combines various elements may therefore have beneficial effects in breast cancer patients. The purpose of this study is to investigate whether a 6-week tango module is suited as a therapeutic approach for people after primary breast cancer therapy to favorably influence their quality of life, especially CRF levels. Methods Sixty patients with a diagnosis for stage I-III breast cancer 12-48 months before enrollment and with CRF (age > 18) will be recruited and randomized 1:1 to a tango or a waiting-list group. Movement concepts using elements of Argentine tango (self-awareness, musical and spatial perception, self-perception, playfulness, shared experience) will be examined with the participants during six consecutive weekly one-hour tango sessions. The primary outcome will be the improvement of CRF (German version of the Cancer Fatigue Scale) and the secondary outcomes will be the improvement in sleep quality (Pittsburgh Sleep Quality Index) and quality of life (EORTC-QLQ-C30). Patient reported outcomes will be measured at baseline and six weeks later; follow-up will be performed six, twelve and twenty four months after baseline. An evaluation will be performed by means of descriptive data analyses. Discussion Argentine tango, as a music-based movement therapy, can influence physical, psychological and cognitive skills. The therapeutic use of Argentine tango in the care of breast cancer patients has not yet been reported. It is anticipated that participants receiving the tango module will have improved CRF, sleep and quality of life scores compared to a waitlist control. Trial registration Trial registration number DRKS00021601. Retrospectively registered on 21 August 2020.
Improving Well-Being and Survival in the 6-OHDA Lesion Model of Parkinson´s Disease in Mice: Step-By-Step Protocol
Authors: Guillaumin Adriane , Vlcek Bianca , Wallén-Mackenzie Åsa 
Published: June 2021

Abstract
Background: Parkinson’s disease (PD) is the most common neurodegenerative motor disorder and primarily affects movement control but also a range on non-motor functions. With unknown cause and lack of cure, much research is dedicated to find treatment. PD is characterized by progressive degeneration of midbrain dopamine neurons, primarily those of the substantia nigra pars compacta (SNc). Due to the complexity of the disease, animal models intended to represent symptoms similar to those observed in PD patients are instrumental to advance treatment prospects for PD. Among these, the 6-hydroxydopamine (6-OHDA) lesion model, in which dopaminergic neurons are chemically destroyed, is often favoured. However, while reproducing several features of clinical PD, including motor symptoms, mice exposed to 6-OHDA often suffer systemic dysfunction causing premature death. To avoid unnecessary spill of lives of laboratory mice and to increase the reliability of data obtained, there is a need for improved experimental protocols. Here we tested the effects of three parameters; lowered dose of toxin, careful post-operative care, and shortened interval between injection and sacrifice. Results: A detailed 6-OHDA lesion protocol using lower dose of toxin than commonly seen in the literature alongside careful post-operative care and decreased time post-injection resulted in high survival rate. Successful degeneration of midbrain dopamine neurons was confirmed in the SNc using several markers, and nigrostriatal projections were lost in the lesioned hemisphere. Dopamine neurons of the ventral tegmental area (VTA) were substantially affected as well. Conclusions: We demonstrate parameters that can be improved to increase well-being and survival of mice while preserving characteristic parkinsonian features in the 6-OHDA lesion model. A step-by-step protocol for implementation in any laboratory is provided.
Antimicrobial compounds were isolated from the secondary metabolites of Gordonia, a resident of intestinal tract of Periplaneta americana
Authors: Ma Yan , Xu Minhua , Liu Hancong , Yu Tiantian , Guo Ping , Liu Wenbin , Jin Xiaobao 
Published: June 2021

Abstract
Microbial secondary metabolites are one of the main sources of bioactive natural products. It is estimated that around 75% of all antibiotics are derived from secondary metabolites produced by filamentous actinomycete. Gordonia sp. are members of the actinomycete family, their contribution to the environment improvement and environmental protection by their biological degradation ability, but there are few studies on the antimicrobial activity of their secondary metabolites. Our team isolated a Gordonia strain WA 4–31 with anti-Candida albicans activity from the intestinal tract of Periplaneta americana in the early stage. In this study, we firstly identified the strain WA 4–31 by the morphological characteristics and the phylogenetic analyses, and found that it homologous to a strain of Gordonia from the Indian desert by 100%. Then four compounds, Actinomycin D (1), Actinomycin X2 (2), Mojavensin A (3) and cyclic (leucine-leucne) dipeptide (4) were purified from the ethyl acetate extract of the fermented broth of the strain. The compounds 1–4 had activities against Candida albicans, Aspergillus niger, Aspergillus fumigatus and Trichophyton rubrum. They also had activities against methicillin-resistant Staphylococcus aureus (MRSA) and Escherichia coli.coli in different degree. Interestingly, we found that when Mojavensin A was mixed with compound 4 ratio of 1:1, the activity of the mixture on anti-Candida albicans was better than the single. Besides, compounds 1–3 had varying degrees of antiproliferative activity on CNE-2 cells and HepG-2 cells. These indicated that Gordonia rare actinomycete from the intestinal tract of Periplaneta americana possessed a potential as a source of active secondary metabolites.
Primary Lung Hyalinizing Clear Cell Carcinoma: A Diagnostic Challenge in Biopsy
Authors: zhang yanling , han wen , zhou jun , yong xiang 
Published: June 2021

Abstract
Introduction: Hyalinizing clear cell carcinomas (HCCCs) are rare, low-grade, malignant tumours. They most commonly involve the minor salivary glands of the head and neck. HCCC that occurs in uncommon locations and examining samples from small biopsy pose a diagnostic challenge for most pathologists.Case presentation: We herein report a primary pulmonary HCCC diagnosed by small biopsy and summarize its histologic, immunophenotypic, and molecular features along with a review of 11 previously reported cases to emphasize the potential diagnostic pitfalls.Conclusions: Small biopsy diagnosis of primary pulmonary HCCC is fall from ideal. A collection of mimics needed to be ruled out. Awareness of the key morphologic features of pulmonary HCCC combined with essential immunohistochemistry and molecular tests contributes to the correct diagnosis.
Optimization of Carotenoids Production from Camelina Sativa Meal Hydrolysate by Rhodosporidium Toruloides
Authors: BERTACCHI STEFANO , Cantù Chiara , Porro Danilo , Branduardi Paola 
Published: June 2021

Abstract

BackgroundPetrochemical synthetic dominates several markets, and carotenoids are not an exception. Since their applications in the food, feed and cosmetic sectors, carotenoids of natural origin are increasingly requested, but the production needs to be sustainable also in terms of initial feedstock. For these reasons we deployed the carotenogenic yeast Rhodosporidium toruloides to obtain such compounds from Camelina sativa meal, an underrated lignocellulosic biomass. As the process starts from hydrolyzed biomass, we separately optimized enzymatic and biomass loadings, to reduce the overall process costs. ResultsThe best conditions (9% w/v biomass, 0.56% w/wbiomass enzymes) were tested in different settings, in which fermentation was separate or co-current with the hydrolysis, showing similar carotenoids productions. The process was implemented in stirred-tank bioreactors, obtaining 3.6 ± 0.69 mg/L of carotenoids, and showing to be robust towards changes in different parameters. ConclusionsThese data pave the way to evaluate a possible industrialization of this bioprocess, considering the opportunity to optimize the use of different amounts of biomass and enzyme loading. In addition, the test in bioreactor is an additional step to further develop the proposed process.
Cutting Mechanical Study of Pick Cutting Coal Seams with Coal and Rock Interface
Authors: Luo Chenxu , Qaio Junbei 
Published: June 2021

Abstract
Shearer provide an effect solution for mining coal, and the cutting performance of pick largely accouts for the ability of a shearer and mining performance. We conducted pick cutting coal experiments in different seam forms on the coal and rock cutting teasted. According to the rotary cutting mechanical model of single pick cutting coal seam, combined with the strength condition of coal seam and coal-rock interface, the rotary cutting mechanical model of pick cutting coal seam with coal-rock interface is established. The stress strain and strength condition of the area in and around the interface are analyzed based on the coal-rock interface crushing theory, which provides basis for further research on the cutting mechanical model of single pick crossing the coal-rock interface. According to the analysis on the ampulitude domain, the phenomenon that force increment between the pick cutting rock and uniform coal seam linearly increases with the increase of compressive strength difference between coal seam and coal-rock interface, and the load fluctuation keep a positive correlation with the compressive strength. Analysis on the signals of the pick cutting coal seam with coal and rock interface at different conditions shows that some basic properties of the cutting load changes over times. In addition, the coal seam with coal-rock interface appears larger impact load and other time-domain characteristics.
CNN with Multiple Input for automatic glaucoma assessment using Fundus Images
Authors: ELMOUFIDI Abdelali , Jai-andaloussi Said 
Published: June 2021

Abstract
In the area of ophthalmology, glaucoma affects an increasing number of people. It is a major cause of blindness. Early detection avoids severe ocular complications such as glaucoma, cystoid macular edema, or diabetic proliferative retinopathy. Intelligent artificial has been confirmed beneficial for glaucoma assessment. In this paper, we describe an approach to automate glaucoma diagnosis using funds images. The setup of the proposed framework is, in order: The Bi-dimensional Empirical Mode Decomposition (BEMD) algorithm is applied to decompose the Regions of Interest (ROI) to components (BIMFs + residue). CNN architecture VGG19 is implemented to extract features from decomposed BEMD components. Then, we fuse the features of the same ROI in a bag of features. These last are very long; therefore, Principal Component Analyses (PCA) are used to reduce features dimensions. Obtained bags of features are the input parameters of the implemented classifier based on the Support Vector Machine (SVM). To train the built models, we have used two public datasets, which are ACRIMA and REFUGE. For testing our models, we have used a part of ACRIMA and REFUGE plus four other public datasets, which are RIM-ONE, ORIGA-light, Drishti-GS1, and sjchoi86-HRF. The overall accuracy of 98.31%, 98.61%, 96.43%, 96.67%, 95.24%, and 98.60% are obtained on ACRIMA, REFUGE, RIM-ONE, ORIGA-light, Drishti-GS1, and sjchoi86-HRF datasets, respectively, by using the model trained on REFUGE. Against an accuracy of 98.92%, 99.06%, 98.27%, 97.10%, 96.97%, and 96.36% are obtained on ACRIMA, REFUGE, RIM-ONE, ORIGA-light, Drishti-GS1, and sjchoi86-HRF datasets, respectively, using the model training on ACRIMA. Obtained experimental results from different datasets demonstrate the efficiency and robustness of the proposed approach. A comparison with some recent previous work in the literature has shown a significant advancement in our proposal.
Chemogenetic stimulation of hippocampal adult-born neurons improves long-term memory accuracy
Authors: lods marie , Mortessagne Pierre , Pacary Emilie , Terral Geoffrey , Farrugia Fanny , Mazier Wilfrid , Masachs Nuria , Charrier Vanessa , Cota Daniela , Ferreira Guillaume , Abrous Djoher , Tronel Sophie 
Published: June 2021

Abstract
Hippocampal adult neurogenesis is involved in many memory processes from learning, to remembering and forgetting. However, whether or not the stimulation of adult neurogenesis can improve memory performance remains unclear. Here, using a chemogenetic approach that combines selective tagging and specific activation of distinct adult-born neuron populations, we demonstrate that this activation can improve remote memory accuracy and strength. These results open up new avenues for remedying memory problems that may arise over time.
Hyperintense Signal on Diffusion-Weighted Imaging for Monitoring The Acute Response and Local Recurrence After Photodynamic Therapy in Malignant Gliomas
Authors: Fujita Yuich , Nagashima Hiroaki , Tanaka Kazuhiro , Hashiguchi Mitsuru , Itoh Tomoo , Sasayama Takashi 
Published: June 2021

Abstract
Purpose

Photodynamic therapy (PDT) subsequent to surgical tumor removal is a novel localized treatment for malignant glioma that provides effective local control. The acute response of malignant glioma to PDT can be detected as linear transient hyperintense signal on diffusion-weighted imaging (DWI) and a decline in apparent diffusion coefficient (ADC) values without symptoms. However, their long-term clinical significance has not yet been examined. The aim of this study was to clarify the link between hyperintense signal on DWI as an acute response and recurrence after PDT in malignant glioma.
Methods

Thirty patients (16 men; median age, 60.5 years) underwent PDT for malignant glioma at our institution between 2017 and 2020. We analyzed the signal changes on DWI after PDT and the relationship between these findings and the recurrence pattern.
Results

All patients showed linear hyperintense signal on DWI at the surface of the resected cavity from day 1 after PDT. These changes disappeared in about 30 days without any neurological deterioration. During a mean post-PDT follow-up of 14.3 months, 19 patients (63%) exhibited recurrence: 10 local, 1 distant, and 8 disseminated. All of the local recurrences arose from areas that did not show hyperintense signal on DWI obtained on day 1 after PDT.
Conclusion

The local recurrence in malignant glioma after PDT occurs in an area without hyperintense signal on DWI as an acute response to PDT. This characteristic finding could aid in the monitoring of local recurrence after PDT.
Control tactics and evaluation of clinical efficacy of therapy in children with bronchial asthma associated with mycoplasma infection
Authors: Gorina L. G. , Krylova N. А. , Rakovskaya I. V. , Goncharova S А. , Barkhatova O. I. 
Published: June 2021

The uncontrolled course of bronchial asthma (BA) in children and insufficient efficacy of standard therapy regimens may be due to underestimated infectious factors. The objective: to study specific parameters of the course and treatment of mycoplasma infection, improve monitoring over BA therapy in children of the tender and preschool age. Subjects and methods. 320 children with BA in the age from 1 to 7 years old were followed up. In this work, Mycoplasma pneumoniae (FH), Mycoplasma hominis (H-34), Ureaplasma urealyticum (serotype 8), Mycoplasma fermentans (PG18) and Mycoplasma arthritidis (PG6) were used, they were cultured on a liquid medium for cultivation of mycoplasmas and ureaplasmas. To isolate CIC from blood serum samples, we used the method of precipitation with 3.5% polyethylene glycol (PEG, 6000 Da), hemagglutination assays and IFA were used to identify mycoplasma antigens, mycoplasma DNA was detected by PCR with InterLabService diagnostic kits. The data of 47 patients with prolonged mycoplasma antigenemia were assessed at the baseline and in 1.5-3 months after the treatment course of azithromycin.Results. 320 blood serum samples from children with BA were tested, and the detection rate by hemagglutination assays of M. pneumoniae antigens was 60.9%, M. hominis – 43.4%, U. urealyticum – 44.8%, M. arthritidis – 29.7%, M. fermentrans – 45.3%. The assessment of relationship between of M. pneumoniae, M. hominis and asthma exacerbation showed that antigens of M. pneumoniae and M. hominis were found in 216 children (single or associated). After treatment with azithromycin, the frequency of BA exacerbations within 3 months decreased by 2.4 times, as well as there was a reduction in the number of samples positive for antigens and DNA of mycoplasma in a free state and within CIC. The persistence of antigens, DNA of M. pneumoniae and M. hominis before treatment of 47 children was 80.9 and 66.0% of cases, after treatment with azithromycin – 31.9 and 25.5% of cases, respectively (p < 0.001). Within CIC isolated from the blood serum of patients, antigens to M. pneumoniae and M. hominis before treatment were detected by IFA in 63.8 and 70.2% of children, after treatment – in 31.9 and 23.4%, respectively. p < 0.001. In blood samples, DNA of M. pneumoniae and M. hominis was detected by PCR before treatment in 8.5 and 34.0%; after treatment in 6.4% (p = 0.318) and 19.1% of cases, respectively (p = 0.009), and within CIC isolated from blood serum, in 27.7 and 48.9% of cases before treatment and 8.5 and 34.0% after it, respectively (p = 0.009).
Pressure-induced structural phase transitions in natural kaolinite investigated by Raman spectroscopy and electrical conductivity
Authors: Hong Meiling , Dai Lidong , Hu Haiying , Zhang Xinyu 
Published: June 2021

Abstract
We investigated the structural, vibrational, and electrical transport properties of natural kaolinite and its high-pressure polymorphs by Raman scattering and electrical conductivity measurements at 293–673 K and up to 10.0 GPa using diamond-anvil cell. Upon compression, kaolinite underwent two structural transitions from kaolinite I to kaolinite II to kaolinite III phases at pressures of 2.9 and 6.5 GPa, respectively, which was disclosed by the inflection point in the pressure-dependent Raman shifts and electrical conductivity. Upon decompression, kaolinite III directly transformed to kaolinite I at 0.8 GPa without the appearance of kaolinite II. Additionally, the influence of temperature on the structural transformation of natural kaolinite was explored by high-temperature and high-pressure electrical conductivity measurements and negative temperature-dependent transition pressure correlations were obtained. A phase diagram of natural kaolinite was established for the first time and the kaolinite I-kaolinite II and kaolinite II-kaolinite III phase transition boundaries were determined: P (GPa) = 4.298–0.00462 T (K) and P (GPa) = 8.895–0.00799 T (K), respectively. Furthermore, our acquired phase diagram can be applied to understand the stability field of high-pressure polymorphs of kaolinite in the Earth’s interior and may provide a phase transition model for other kaolin-group minerals.
Global Biomaterials Market: Potential Opportunities for Raw Materials of Animal Origin
Authors: Patshina Marina , Voroshilin Roman , Osintsev Alexey 
Published: June 2021

Introduction. Meat processing enterprises are facing the problem of utilization of secondary products with a limited scope of application and a wide range of useful properties. One of the promising ways of slaughter-house by-product utilization is the production of biomaterials that can replace various tissues of a living organism. This review presents an analysis of the global biomaterials market, its volume, and possible ways of using secondary meat resources in various sectors of economy.
Study objects and methods. The article represents some results of a desk research based on open sources, i.e. publications and Internet data portals.
Results and discussion. One of the most important tasks of modern regenerative medicine is to develop fast and effective methods for the restoration of damaged or lost organs and tissue fragments. Its solution directly depends on new advanced biomaterials. Modern biocompatible materials are in great demand in such areas of medicine as orthopedics, ophthalmology, dentistry, general and cardiovascular surgery, restorative medicine, drug delivery, etc. Cosmetology is a rapidly evolving segment of medicine and depends on such biomaterials as hyaluronic acid and collagen. Russian biomedicine occupies 0.7% of the world market. However, the Russian segment is likely to grow and expand its range of biomaterials.
Conclusion. The currently unused resources of meat industry can be an excellent source of valuable raw materials for the advanced biomedical structures used in tissue engineering. A wide variety of structures and properties of secondary resources can produce a wide range of biomaterials. The possibility of manufacturing matrices from internally sourced raw materials within one enterprise is particularly promising.