bluuebunny/crossref_metadata_2025_split huggingface dataset:
CONHECIMENTO DO ENFERMEIRO SOBRE OS DEPENDENTES QUÍMICOS NO CENTRO DE REABILITAÇÃO EM DEPENDÊNCIA QUÍMICA (CRDQ) – ISMAEL ABDEL AZIZ
Authors: Mazalo João Viriato , Conceição Antônio Marcos Sales , Mori Bruno 
Published: October 2021

RESUMO
Os enfermeiros desempenham um papel crucial na transformação social, tendo atuação ativa no processo de tratamento, reabilitação, combate ao consumo de álcool e outras drogas psicoativas. O consumo de drogas é considerado um problema de saúde pública, que afeta todas as faixas etárias, sem distinção da classe social, criando implicações sociais, psicológicas, econômicas e políticas. Esta pesquisa tem como objetivo avaliar o conhecimento dos enfermeiros sobre os dependentes químicos no Centro de Reabilitação em Dependência Química – Ismael Abdel Aziz. Trata-se de um estudo descritivo-exploratório de natureza qualitativa. Participaram no estudo 6 enfermeiros que atuavam na instituição. A coleta de dados foi realizada no mês de julho de 2015, com base em entrevista semiestruturada, sendo categorizados segundo a análise de conteúdo de Bardin. Os enfermeiros afirmaram que a demanda dos dependentes químicos tende a crescer progressivamente devido a vários fatores expostos pela sociedade, havendo necessidade de maior abrangência de políticas públicas e criação de novos projetos, bem como a capacitação dos enfermeiros para melhorar o atendimento. Após a realização da pesquisa concluiu-se que os enfermeiros possuem conhecimentos básicos que são utilizados no processo de recuperação dos dependentes químicos, onde estes atuam na assistência das crises de abstinências, fissura, ansiedade, entre outras situações expostas.
Palavras-chave: Dependência Química; Drogas; Reabilitação.
DNA Hypermethylation Modification Promotes the Development of Hepatocellular Carcinoma by Depressing the Tumor Suppressor Gene ZNF334
Authors: Sun Da-peng , Gan Xiao-jie , Liu Lei , Yang Yuan , Ding Dong-yang , Li Wen , Jiang Junyao , Ding Wen-Bin , Zhao Ling-hao , Hou Guo-jun , Yu Jian , Wang Jie , Zhou Wei-ping , Yang Fu , Yuan Sheng-xian 
Published: October 2021

Abstract
Background: DNA methylation plays a pivotal role in the development and progression of tumors, but studies focused on the dynamic changes of DNA methylation in the development of hepatocellular carcinoma (HCC) are rare. This manuscript is aimed to construct pre- and early DNA methylation maps of liver cancer of the same genetic background, as well as to reveal the mechanism of epigenetics regulating gene expression during the development of liver cancer, thus providing new targets and clinical evidence for early diagnosis and shedding lights on the precise treatment for liver cancer. Methods: The study includes 5 patients who were chronic hepatitis B virus infected, clinically diagnosed as primary liver cancer and pathologically diagnosed as early liver cancer with liver dysplastic nodules. Liver fibrosis tissues, dysplastic nodules and early HCC tissues from these patients have been used to measure DNA methylation. Results: We report significant differences in the DNA methylation spectrum of three types of tissues. In the early stage of HCC, DNA hypermethylation of tumor suppressor genes is predominant. Additionally, DNA hypermethylation in the early stage of HCC changes the binding of transcription factor P53 to the promoter of tumor suppressor gene ZNF334, and inhibits the expression of ZNF334 at the transcription level. Furthermore, through a series of in vivo and in vitro experiments, we have clarified the exacerbation effect of tumor suppressor gene ZNF334 deletion in the occurrence of HCC. Combined with clinical data, we found that the overall survival and disease-free survival of patients with high ZNF334 expression are longer than the lower one. Conclusions: We constructed a sequential map of DNA methylation modification during the occurrence of HCC, and clarified the biological function and regulatory mechanism of the tumor suppressor gene ZNF334, which is regulated by related DNA methylation sites, and also provide new targets and clinical evidence for the early diagnosis and precise treatment of liver cancer.
Simulation Research on PEMFC fueled with ammonia reforming gas
Authors: Zhao Jianfeng , LIANG Qianchao , LIANG Yifan , Jinyi HU 
Published: October 2021

Abstract
Ammonia as a chemical storage carrier for hydrogen has the advantages of high hydrogen storage density and easy storage. However, reconversion of ammonia to pure hydrogen requires additional energy consumption and complex equipment, thus greatly reducing its advantages as a hydrogen storage carrier. The development of PEMFC that use ammonia decomposition gas directly as fuel can avoid the purification of hydrogen and simplify on-site hydrogen production systems. Thus, the application of fuel cells can be greatly expanded. In this paper, a three-dimensional simulation model of PEMFC using ammonia reforming gas as fuel is established based on experimental data. The distribution of local current density and local hydrogen concentration inside the fuel cell and the relationship between them are studied and analyzed. The optimal efficiency and fuel utilization of the fuel cell under different flow rates are investigated. Finally, a preliminary simulation analysis of the effect of anode runner length on the maximum output power of the fuel cell is conducted. The results of the study can provide a reference for the fuel control strategy of ammonia reforming gas-fueled PEMFC and the optimization of the fuel cell.
Validity, Agreement and Accuracy of A Hand-Held Dynamometer for Shoulder Muscles Strength Assessment in Healthy Individuals
Authors: NEPOMUCENO BALBINO , Neto Mansueto Gomes , Schindler Ingara Silva Ribeiro , Machado Mariana , de Melo Thiago 
Published: October 2021

Abstract
Background: The shoulder complex has a high prevalence of injuries. It is fundamental to quantify the muscle strength and identify muscular imbalances that predispose to lesions. The aim was to test concurrent validation of the muscle strength assessment with a hand held dynamometer (HHD) for shoulder joint muscles, and measure the accuracy and diagnostic agreement between instruments for assessing the strength of this joint with the hand held dynamometer and isokinetic dynamometer (ID) in a population of healthy individuals Methods: healthy individuals aged between 18 and 40 years were included. The HHD was used to test the isometric contraction of the main shoulder movements. Isometric contraction was performed for 3 seconds. Assessments with HHD and DI were performed on the same day, with a minimum interval of 90 minutes between tests. The study was approved by the Ethics Committee by report No.1537948. Concurrent validation was calculated with Pearson’s correlation, the accuracy obtained by the ROC curve and agreement by Kappa test. Results: The HHD showed concurrent validation between 0.51 – 0.83, with sensitivity between 0.90 and 0.98 and specificity between 0.64 and 0.89 for shoulder movements. The HHD demonstrated moderately-strong to excellent concurrent validity. Conclusion: These results are encouraging for the routine use of this portable and lower cost instrument in quantification of the pique torque of the shoulder muscles. Moreover, this instrument showed good accuracy and moderate to high agreement in comparison with diagnosis of the gold standard instrument.
The Antihyperalgesic Effects of the Calcium Channel Blocker CTK 01512-2 Toxin in Persistent Pain Models
Authors: Cavalli Juliana , Gonçalves Elaine Cristina Dalazen , Bobermin Larissa Daniele , Quincozes-Santos André , Gomez Marcus Vinicius , Dutra Rafael Cypriano 
Published: October 2021

Abstract
The CTK 01512-2 toxin is a recombinant peptide of the Phα1β version derived from the venom of the Phoneutria nigriventer spider. It is an N-type voltage-gated calcium channel (VGCC) blocker showing a prolonged effect on the prevention and reduction of nociception. Herein, CTK 01512-2 toxin was tested in two models of persistent pain, the chronic post-ischemia pain (CPIP) and the peripheral neuropathy induced by paclitaxel, to evaluate its intrathecal, systemic, and intracerebroventricular effects on mechanical hypersensitivity and thermal allodynia. The astroglial cell viability using the MTT test was also investigated. The results showed that CTK 01512-2 intrathecal and systemic treatments reduced mechanical hypersensitivity induced by CPIP, mainly between 1 – 4 h after CTK 01512-2 administration. When the CTK 01512-2 intrathecal treatment was applied, it also reduced the thermal allodynia induced by CPIP. CTK 01512-2 intracerebroventricular treatment also showed mechanical antihyperalgesic and thermal antiallodynic effects in the peripheral neuropathy induced by paclitaxel, reinforcing CTK 01512-2 therapeutically potential to treat persistent pain conditions. CTK 01512-2 further altered the astroglial cell viability in the MTT reduction assay, which may indicate its effects on the mitochondrial activity of these cells, possibly as a compensatory mechanism on calcium signaling. The current study shows CTK 01512-2 antihyperalgesic effects in persistent pain models, helping to pave new perspectives of pain relief treatments to patients affected by peripheral neuropathy and chronic pain conditions.
Anatomical and Symptomatic Outcomes in Patients With Le Fort Colpocleisis
Authors: KINCI Mehmet Ferdi , SEZGİN Burak , ARSLANER Mehmet Onur , GÖKBEL Deniz AKIN , GÖKBEL İsmail , SİVASLIOĞLU Ahmet Akın 
Published: October 2021

Abstract
Background: We aimed to evaluate the short term anatomical and symptomatic results of elderly patients on whom Le Fort colpocleisis operation was performed due to pelvic organ prolapse (POP) in our clinic. Methods: Medical records of fifty-nine sexually inactive and ≥65 aged women with stage 2 or higher vaginal or uterine prolapse who underwent Le Fort colpocleisis operation were prospectively analysed. Pre-operative and 12 months post-operative data were recorded. Turkish validated Global Pelvic Floor Bother Questionnaire (GPFBQ) were also evaluated at pre-operative and 12 months post-operative period in all patients. Anatomical success was determined as no prolapse of any POP-Q point at or below 1 cm above the hymen. Results: A total of 59 patients were conducted in this study. The average age of the patients was 71.67±7.01 (years). The mean BMI was 27.1±9.52 kg/m2. POP-Q point C as well as, Gh and TVL measurements were significantly higher after surgery than those at preoperative period (p<0.01, p<0.01, p<0.01, respectively). There was no recurrence in any case. Evaluation of complications at the pre-operative and post-operative 12th month revealed significant differences for SUI, urinary frequency, nocturia, and pelvic pain symptoms during post-operative period (p:0.007, p<0.001, p:0.01, p<0.001; respectively). Conclusions: Le Fort colpocleisis is a simple and effective procedure that has been found to provide successful anatomical and symptomatic outcomes in sexually inactive and elderly women for POP. However long-term results of this procedure needs further investigation.
Research On Adaptive Optimal Iterative Learning Control Based on Least Squares
Authors: Yang Liang-Liang , Luo Xiang , Yuan Rui , Zhang Hui 
Published: October 2021

Abstract
Traditional Optimal Iterative Learning Control (TOILC) can effectively improve the tracking performance of the servo system. However, there may be parameter perturbation in the running process of the servo system, and its parameters are constantly changing slowly. As a result, the convergence of TOILC becomes worse, and the tracking performance of the system deteriorates seriously. Therefore, in view of the time-varying characteristics of the system, a least squares optimal iterative learning control (LSAOILC) algorithm is proposed. In the process of iteration, the nominal model of the system is identified according to the input and output signals so as to update the optimal iterative learning controller, which does not need to obtain the exact system model information in advance, making up for the shortage of TOILC. The simulations and experiments prove the effectiveness of the proposed strategy for the servo system.
How Atoms of Polycrystalline Nb 20.6 Mo 21.7 Ta 15.6 W 21.1 V 21.0 refractory High-Entropy Alloys Rearrange during the Melting Process
Authors: Ju Shin-Pon , Li Chen-Chun 
Published: October 2021

Abstract
The melting mechanism of single crystal and polycrystalline Nb 20.6 Mo 21.7 Ta 15.6 W 21.1 V 21.0 RHEAs was investigated by the molecular dynamics (MD) simulation using the 2NN MEAM potential. For the single crystal RHEA, the density profile displays an abrupt drop from 11.25 to 11.00 g/cm 3 at temperatures from 2910 to 2940 K, indicating all atoms begin significant local structural rearrangement. For polycrystalline RHEAs, a two-stage melting process is found. In the first melting stage, the melting of the grain boundary (GB) regions firstly occurs at the pre-melting temperature, which is relatively lower than the corresponding system-melting point. At the pre-melting temperature, most GB atoms have enough kinetic energies to leave their equilibrium positions, and then gradually induce the rearrangement of grain atoms close to GB. In the second melting stage at the melting point, most grain atoms have enough kinetic energies to rearrange, resulting in the chemical short-ranged order (CSRO) changes of all pairs.
Revealing key lncRNAs in cytogenetically normal acute myeloid leukemia by reconstruction of the lncRNA–miRNA–mRNA network based on the competitive endogenous RNA theory
Authors: Sun Tao , Dong Lin , Guo Yan , Zhao Hai , Wang Manzhi 
Published: October 2021

Abstract
Background: Cytogenetically normal acute myeloid leukemia(CN-AML) is a heterogeneous disease with different prognosis.Researches on prognostic indicators and therapy targets of CN-AML are still ongoing.Instead of protein-coding genes,more and more researches were focused on the non-coding RNAs especially long non-coding RNAs(lncRNAs) which may play an important role in the development and prognosis of AML.Although a large number of lncRNAs had been found, our knowledge of their function and pathological significance is still in its infancy.The purpose of this research is to identify the key lncRNAs and explore their function in CN-AML by reconstructing the lncRNA–miRNA–mRNA network based on the competitive endogenous RNA(ceRNA) theory. Results: We reconstructed a global triple network based on the ceRNA theory using the data from National Center for Biotechnology Information Gene Expression Omnibus and published literature. According to the topological algorithm,we identified the key lncRNAs which had both the higher node degrees and the higher number of lncRNA–miRNA and miRNA–mRNA pairs in the ceRNA network. Meanwhile, Gene Ontology (GO) and pathway analysis were performed using databases such as DAVID,KOBAS and Cytoscape plug-in ClueGO respectively.The lncRNA–miRNA–mRNA network was composed of 90 lncRNAs,33mRNAs,26 miRNAs and 259 edges in the lncRNA upregulated group,and 18 lncRNAs,11 mRNAs,6 miRNAs and 45 edges in the lncRNA downregulated group.The functional assay showed that 53 pathways and 108 GO terms were enriched. Three lncRNAs(XIST,GABPB1-AS1,TUG1)could possibly be selected as key lncRNAs wihch may play an important role in the development of CN-AML.Particularly,GABPB1-AS1 was highly expressed in CN-AML by both bioinformatics analysis and experimental verification in AML cell line(THP-1) by quantitative real‐time polymerase chain reaction.In addition, GABPB1-AS1 was also negatively correlated with overall survival of AML patients. Conclusion: The lncRNA–miRNA–mRNA network revealed key lncRNAs and their functions in CN-AML.Particularly,lncRNA GABPB1-AS1 was firstly proposed in AML.We believe that GABPB1-AS1 is expected to become a candidate diagnostic biomarker or potential therapeutic target.
Promoting the adoption of local governmental policy on the reimbursement of chronic disease medicines (PAPMed): study protocol of a field-based cluster randomized trial in rural Nantong, China
Authors: He Zhengting , Cao Xin , Zhao Duan , Tang Zemin , Zhao Jiayu , Beasley Mariel , Renne Angela , Gao Yuexia , Yan Lijing L. 
Published: October 2021

Abstract
Background

Among rural Chinese patients with non-communicable diseases (NCDs), low socioeconomic status increases the risk of developing NCDs and associated financial burdens in paying for medicines and treatments. Despite the chronic disease medicine reimbursement policy of the local government in Nantong City, China, various barriers prevent patients from registering for and benefitting from the policy. This study aims to develop a behavior-science based intervention program for promoting the adoption of the policy and to evaluate the effectiveness of the program compared with usual practices.
Methods

Barriers and opportunities affecting stakeholders in adopting the policy were identified through contextual research and summarized through behavior mapping. The intervention targets these barriers and opportunities through behavior science theories and will be evaluated through a 6-month cluster randomized controlled trial in Tongzhou District, Nantong, China. A total of 30 villages from two townships are randomized in a 1:1 ratio to either the intervention or the control arm (usual practices). Village doctors in the intervention arm (1) receive systematic training on policy details, registration procedures, and intervention protocol, (2) promote the policy and encourage registration, (3) follow-up with patients in the first, third and sixth month after the intervention, and (4) receive financial incentives based on performance. The primary outcome is policy registration rate and the secondary outcomes include the number of patients registering for the policy, medical costs saved, frequency of village doctor visits, and health conditions such as blood pressure and glucose levels.
Discussion

This study is one of very few that aims to promote adoption of NCDs outpatient medication reimbursement policies, and the first study to evaluate the impact of these policies on patients’ financial and physical wellbeing in China. The simple, feasible, and scalable intervention is designed based on the theories of behavior science and are applicable to similar low-income regions nationwide where outpatient medical costs remain a financial burden for patients.
Trial registration:

ClinicalTrials.gov, NCT04731194, registered on 29 January 2021, https://clinicaltrials.gov/ct2/show/NCT04731194?term=PAPMed&draw=2&rank=1; Chinese Clinical Trial Registry, ChiCTR2100042152, registered on 14 January 14 2021, http://www.chictr.org.cn/showproj.aspx?proj=65987.
CRISPR / Cas-9 Vector System: Targets UL-39 and Inhibits HSV-1 Replication in Vitro
Authors: Raposo Jéssica Vasques , Barros Luciana Rodrigues Carvalho , Torres Lauana Ribas , Pinto Rafaela Barbosa da Silva , Lopes Amanda de Oliveira , de Souza Elen Mello , Bonamino Martin Hernan , de Paula Vanessa Salete 
Published: October 2021

Abstract
HSV-1 aﬀects approximately 67% of the world population. Here, we sought to use the CRISPR / Cas9 system with the UL39 target, essential for virus replication. The sgRNA sequence was inserted into plasmid (PX459-UL39). Vero cells were transfected with PX459-UL39, and inhibition of viral replication was assessed 24 and 48 hours later using plaque assays and ﬂuorescence and qPCR. Fluorescence analyzes revealed the presence of anti-HSV-1 CRISPR/Cas9 within Vero cells, and qPCR showed that the viral load decreased by> 95% of cells transfected with anti-HSV-1 CRISPR / Cas9. Our data demonstrate the usefulness of the PX459-UL39 to inhibit HSV-1 infection.
Can The Charcoal Localization For Surgical Resection of Non-Palpable Breast Suspicious Lesions Be a Promising Method For Communities of Low Resources? Retrospective Observational Study.
Authors: Farouk Omar , Ezzat Mohamed , El-Badrawy Adel , Fady Tamer , Abdallah Ahmed , El-Kashef Wagdi , Shams Nazem , Senbel Ahmed 
Published: October 2021

Abstract
Background: Breast cancer is more frequently being detected as radiographic non-palpable lesions with the increased utilization of national screening programs. Moreover, the sizes of tumors detected have decreased in recent years, increasing the need for the accurate image-directed localization for surgical excision in a significant portion of cases. Although Wire guided localization has been the most commonly used method for many years, but inherent problems remain and limited its practice. Radio-guided occult lesion localization (ROLL) is currently the standard method of localization, however it is unavailable in most of low resources communities. This encourages us to use charcoal localization which is simple and cheap method of surgical localization of non-palpable suspicious breast lesions. Methods: This prospective study included 34 patients presented with non-palpable suspicious breast lesion (BIRADS 4 or 5). All patients were injected with 1-3 ml of sterilized 3% aqueous suspension of charcoal granules under guidance of ultrasound at the superficial border of the suspicious lesion and the track between the lesion and the needle entry point in the skin which occur at the future incision. This method was carried out in most patients one day before operation, however two patients underwent surgical excision after 6 days of localization without any interruption.Results: Thirty four patients had 36 Lesions. The median age was 43 years. The mean diameter of lesions 10.9 mm. Out of 36 lesions; the BIRADS as follow10 (4a), 12 (4b), 8 (4c), and 6 (5). Postoperative revealed 16 malignant lesions and 20 benign lesions. All 20 benign lesions were managed by wide local excision; All 14 BIRADS 4a lesions were proved to be benign. Sixteen malignant lesions were managed as the following; nine patients had breast conserving surgery, five patients had modified radical mastectomy (three patients had past history of modified radical mastectomy, one patient had Multicentric ILC and one patient had infiltrated safety margins on conservation), and one patient had Nipple Sparing Mastectomy with immediate breast reconstruction by Latissimus Dorsi Flap . There was no reaction nor infection reported in our study.Conclusion: Charcoal localization has many advantages and help surgical localization.
Molecular Dynamics Simulations on the Chain Fold During the Isothermal Orientation of n-Alkanes on Graphene
Authors: Zhang Zhi Meng , Yang Hua , Shi Jun Xia , Wang Jia Jun , Huang Zheng Guo , Zhang Hui 
Published: October 2021

Abstract
The orientation of hydrocarbon chains plays a key role in the applications of organic materials. And chain folding in the process of molecular orientation is also of great significance for the design of organic molecular thin films. The effect of chain length and simulation temperature on the isothermal orientation of n-alkanes on graphene surface is studied by molecular dynamics simulation in this paper. And the chain folding is also described. The n-alkanes can form perpendicular ordered structure, parallel ordered structure or perpendicular orientation at relative low temperature and parallel orientation at relative high temperature on graphene surface. The chain fold happens when long n-alkanes form perpendicular ordered structure on graphene surface. And the simulation results show the interactions of n-alkane−graphene and n-alkane−n-alkane affect chain fold.