bluuebunny/crossref_metadata_2025_split huggingface dataset:
Down-regulated Beclin 1 expression is closely linked to lung carcinogenesis by reversing aggressive phenotypes
Authors: Zhao Shuang , Xue Hang , Jiang Hua-mao , Zheng Hua-chuan 
Published: June 2021

Abstract
Background

Beclin1 is involved in numerous biological processes, including autophagy, stress, senescence, and death. This article aims to clarify the roles of Beclin1 expression in lung carcinogenesis and subsequent progression.
Methods

The Beclin 1 expression was examined in lung cancer and compared it with clinicopathological parameters and survival data of the cancers using a large number of lung cancer samples. Additionally, we observed the effects of forced Beclin 1 overexpression and its silencing on the proliferation, glucose metabolism, apoptosis, autophagy, invasion, migration, lamellipodia formation, and chemoresistance of lung cancer cells and analyzed the relevant mechanisms. Finally, in vivo effects of Beclin 1 overexpression on tumor growth was determined in nude mice.
Results

Beclin 1 reversed aggressive phenotypes in SQ-5 and KJ cells with Bcl-2, HSP90, and β-catenin underexpression, LC-3B, ADFP, p-p38, and cytochrome c hyperexpression. The effect of Beclin 1 silence was opposite in H446 cells. In vivo and in vitro Beclin 1-mediated chemoresistance to cisplatin was close with the apoptotic level and NF-кB activation. Beclin 1 overexpression suppressed tumor growth of lung cancer by decreasing proliferation and inducing apoptosis and autophagy. Beclin 1 expression was down-regulated in lung cancer at both mRNA and protein levels. A higher level of Becn1 mRNA was detectable in adenocarcinoma than squamous cell carcinoma (p < 0.05), while versa for its protein. Becn1 mRNA expression was positively correlated with overall or post-progression survival rates of all cancer patients, even stratified by aggressive behaviors (p < 0.05).
Conclusions

These findings suggested that down-regulated Beclin1 expression was closely linked to the tumorigenesis and histogenesis of lung cancer. Beclin 1 might be employed as a potential target for the gene therapy of lung cancer if its chemoresistance can be ameliorated or avoided.
LINC00839 Knockdown Restrains The Metastatic Behaviors of Nasopharyngeal Carcinoma By Sponging miR-454-3p
Authors: Zhang Feng Ying , Li Xia , Huang Ting Ting , Xiang Mei Ling , Sun Lin Lin , Sun Zhao Lan 
Published: June 2021

Abstract
Background Long intergenic non-coding RNA 00839 (LINC00839) has been verified as a cancer-promoting gene in malignancies. However, the significance of LINC00839 in nasopharyngeal carcinoma (NPC) has yet to be elaborated, as well as its underlying mechanism.Methods LINC00839 and miR-454-3p relative expression levels in NPC cells were examined by qRT-PCR. The growth of cells was examined by CCK-8 and colony formation assays. Cell migration and invasion were examined by wound healing and Transwell experiment, respectively. The binding sequence of LINC00839 and miR-454-3p was confirmed by the luciferase reporter gene experiment. The regulatory function of LINC00839 and miR-454-3p on c-Met was investigated by western blot.Results Here, we revealed that LINC00839 was elevated in NPC. Both LINC00839 knockdown and upregulation of miR-454-3p suppressed NPC cells proliferation, invasive capacity and EMT in vitro. Besides, LINC00839 was validated as a miR-454-3p “sponge”, and upregulation of LINC00839 could reverse miR-454-3p-mediated functions in NPC C666-1 and SUNE-1 cells. Furthermore, c-Met was determined to be targeted by miR-454-3p. Notably, c-Met was downregulated by LINC00839 knockdown through sponging miR-454-3p. In vivo, LINC00839 knockdown resulted in a slower tumor growth.Conclusions Altogether, knockdown of LINC00839 inhibits the aggressive properties of NPC cells via sponging miR-454-3p and regulating c-Met.
CXCL14 Facilitates the Growth and Metastasis of Ovarian Carcinoma Cells via Activation of the Wnt/β-catenin Signaling Pathway
Authors: Gao Li-Na , Hao Man , Liu Xiao-Hui , Zhang Li , Dong Yan , Zhang Yu-Fang , He Xiao-Chun 
Published: June 2021

Abstract
Background There is an urgent need to identify potential targets in anticancer therapy to improve the survival and prognosis of patients with ovarian cancer (OC). Herein, we investigated the functional significance of chemokine (C-X-C motif) ligand 14 (CXCL14) in OC cell growth and epithelial–mesenchymal transition (EMT).MethodsqRT PCR and western blotting was used to detect CXCL14 mRNA level and protein expression, respectively. The functional mechanism of CXCL14 in OC was investigated by CCK-8, colony formation and transwell assays. The protein expression of CXCL14 and β-catenin in OC tissues was determined by immumohistochemical staining.ResultsWe demonstrated that high levels of CXCL14 were associated with a worse prognosis in patients with OC. CXCL14 knockdown considerably restrained the growth and invasion of OC cells in vitro. In contrast, ectopic CXCL14 overexpression yielded the opposite results. Investigations to determine the underlying molecular mechanisms revealed that the Wnt/β-catenin signaling pathway is involved in CXCL14-facilitated OC cell invasiveness.ConclusionThese data collectively demonstrate that CXCL14 contributes to OC cell growth and metastatic potential by regulating the Wnt/β-catenin signaling pathway.
Knowledge of Surgical Informed Consent and Associated Factors among Patients Undergone Obstetric and Gynecologic Surgery at Jimma Medical Center, Jimma, Oromia Region, Ethiopia, 2020. An Institutional Based Cross-Sectional Study.
Authors: Kebede Belete Fenta , Tesfaye Tsegaw Biazin , Hiwot Aynalem Yetwale 
Published: June 2021

Abstract
Introduction: The practice of informed consent before any surgical procedure should form the fundamental element that serves to protect both patient and surgeon from prosecution. Several factors can affect the process of surgical informed consent. Knowledge towards surgical informed consent is one important factor. It is worth mentioning that patients’ understanding of the information provided in the consent form is a prerequisite for obtaining valid informed consent, otherwise, it will be only symbolic. The study aimed to assess knowledge of surgical informed consent and associated factors among patients who undergone obstetric and gynecologic surgery at Jimma Medical Center, Jimma, Oromia Region, EthiopiaMethodology: Institutional based crossectional study was conducted among systematically selected women undergo obstetrics and gynecologic surgery in Jimmy medical center from April 1 to May 30, 2020. The collected data were coded, entered into Epi data version 3.1, and analyzed using SPSS version 25. Bivariate and multivariate regression was carried out to determine the association between an outcome variable and an independent variable. Tables, pie -charts, and texts were used to report the result.Results: This study revealed that the mean age of the respondents was 29.5 ±3.5 years and the majority of them (83.3%) were married. The respondents had significantly poor overall knowledge of surgical informed consent 77.2 %( 95%CI: 72.8-81.2).Patient educational status (AOR: 1.22 (95%CI: 1.1-2.47), satisfaction level (AOR: 1.823(95%CI: 1.061-3.134).and patient- provider relation-ship (AOR: 0.472(95%CI: 0.271-0.822) were associated with knowledge of surgical informed consent.Conclusion: in this study, the overall level of knowledge regarding surgical informed consent was significantly poor than the other national and international figures. Patient Education, satisfaction, and patient-provider relation-ship were associated with knowledge of surgical informed consent. Health care providers should provide adequate information before surgery to improve patients’ knowledge regarding surgical informed consent and provide adequate input on ways to improve the consent process to make it better suited to fit the needs of all patients.
Adsorption and Desorption of Cd: Characteristics and Mechanisms in Reclaimed Soil Under the Influence of Dissolved Organic Carbon
Authors: Zheng Yonghong , Zhang Zhiguo , Chen Yongchun , An Shikai , Zhang Lei , Chen Fangling , Ma Chengnan , Cai Weiqing 
Published: June 2021

Abstract
Organic acids are widespread in the environment, where they play an important role in the adsorption, desorption, and migration of Cd in soil. This study evaluated the characteristics and mechanisms of Cd adsorption and desorption in reclaimed soil from the Panyi Mine, in the Huainan mining area (China), under the influence of humic acid (HA). The addition of HA inhibited the adsorption of Cd but enhanced its desorption in reclaimed soil. With an increasing concentration of added HA, the adsorption capacity of Cd in the reclaimed soil gradually decreased, while the desorption capacity gradually increased. That is, the adsorption inhibition/desorption promotion effect was stronger under higher concentrations of organic acid. Cd adsorption and desorption dynamics could be described as a logarithmic function of organic acid concentration. Kinetic curves for Cd adsorption and desorption in reclaimed soil under the influence of organic acid showed that both adsorption and desorption involved two stages: a fast reaction stage, followed by a slow reaction stage. The factors influencing the capacity of Cd adsorption and desorption in soil were analyzed by gray correlation analysis, and their resulting rank order was as follows: Cd concentration > HA concentration > pH > temperature. Using linear regression analysis, a multi-factor coupling functional model of soil Cd adsorption and desorption under the influence of DOC was established, in the form of Y = a + bXHA + cXT + dXPH + eXCd.
Evidence supporting deployment of next generation insecticide treated nets in Burkina Faso: bioassays with either chlorfenapyr or piperonyl butoxide increase mortality of pyrethroid-resistant Anopheles gambiae s.l.
Authors: HIEN Sawdetuo Aristide , Soma Dieudonné D. , Coulibaly Dramane , Diabaté Abdoulaye , Belemvire Allison , Diouf Mame B , Jacob Djenam , Koné Adama , Dotson Ellen , Awolola Taiwo S. , Oxborough Richard M. , Dabiré Roch K. 
Published: June 2021

Abstract
Background

Pyrethroid resistance poses a major threat to the efficacy of insecticide treated nets (ITNs) in Burkina Faso and throughout sub-Saharan Africa, particularly when resistance is present at high intensity. For such areas there are alternative ITNs available, including the synergist piperonyl butoxide (PBO)-based ITNs and dual active ingredient ITNs such as Interceptor G2 (treated with chlorfenapyr and alpha-cypermethrin). Before deploying alternative ITNs on a large scale it is crucial to characterize the resistance profiles of primary malaria vector species for evidence-based decision making
Methods

Larvae from the predominant vector, Anopheles gambiae s.l., were collected from 15 sites located throughout Burkina Faso and reared to adults for bioassays to assess insecticide resistance status. Resistance intensity assays were conducted using WHO tube tests to determine the level of resistance to pyrethroids commonly used on ITNs at 1x, 5x and 10x times the diagnostic dose. WHO tube tests were also used for PBO synergist bioassays with deltamethrin and permethrin. Bottle bioassays were conducted to determine susceptibility to chlorfenapyr at a dose of 100µg/bottle.
Results

WHO tube tests revealed high intensity resistance in An. gambiae s.l. to deltamethrin and alpha-cypermethrin in all sites tested. Resistance intensity to permethrin was either moderate or high in 13 sites. PBO pre-exposure followed by deltamethrin restored full susceptibility in 1 site but partially restored susceptibility in all but one of the remaining sites (often reaching mortality greater than 80%). PBO pre-exposure followed by permethrin partially restored susceptibility in 12 sites. There was no significant increase in permethrin mortality after PBO pre-exposure in Kampti, Karangasso-Vigué or Mangodara; while in Seguenega, Orodara and Bobo-Dioulasso there was a significant increase in mortality, but rates remained below 50%. Susceptibility to chlorfenapyr was confirmed in 14 sites.
Conclusion

High pyrethroid resistance intensity in An. gambiae s.l. is widespread across Burkina Faso and may be a predictor of reduced pyrethroid ITN effectiveness. PBO + deltamethrin ITNs would likely provide greater control than pyrethroid nets. However, since susceptibility in bioassays was not restored in most sites following pre-exposure to PBO, Interceptor G2 may be a better long-term solution as susceptibility was recorded to chlorfenapyr in nearly all sites. This study provides evidence supporting the introduction of both Interceptor G2 nets and PBO nets, which were distributed in Burkina Faso in 2019 as part of a mass campaign.
EfficientNetB3 Architecture for Diabetic Retinopathy Assessment using Fundus Images
Authors: ELMOUFIDI Abdelali , Amoun Hind 
Published: June 2021

Abstract
Classification of the stages of diabetic retinopathy (DR) is considered a key step in the assessment and management of diabetic retinopathy. Due to the damage caused by high blood sugar to the retinal blood vessels, different microscopic structures can be occupied in the retinal area, such as micro-aneurysms, hard exudate and neovascularization. The convolutional neural network (CNN) based on deep learning has become a promising method for the analysis of biomedical images. In this work, representative images of diabetic retinopathy (DR) are divided into five categories according to the professional knowledge of ophthalmologists. This article focuses on the use of convolutional neural networks to classify background images of DR according to disease severity and on the application of pooling, Softmax Activation to achieve greater accuracy. The aptos2019-blindness-detection database makes it possible to verify the performance of the proposed algorithm.
Effect of Licorice and Green Tea Gargle on Post-Extubation Sore Throat, Cough, and Hoarseness: A Triple-blind Clinical Trial
Authors: Karimi Hamid , Rajai Nahid , Farsi Zahra , Hashemi Parisa 
Published: June 2021

Abstract
Background

Endotracheal extubation is associated with a high prevalence of complications such as sore throat, cough, and hoarseness. This study aimed to compare the effect of green tea and licorice gargle on sore throat, cough, and hoarseness after endotracheal extubation.
Method:

The study was designed as a randomized, triple-blind, controlled clinical trial. 102 patients who were candidates for elective surgery in two specialized hospitals in Tehran, Iran in 2020–2021, were randomly divided into licorice, green tea, and control (placebo) groups. The scores of sore throat, cough, and hoarseness of patients were assessed one hour after endotracheal extubation. After the gag reflex returned, patients were given 100 cc of the prepared solutions to gargle for 30 seconds. The intervention was repeated two hours later. The scores of sore throat, cough, and hoarseness were assessed in the three groups, immediately after the first intervention and two hours after the second intervention.
Result

There was a significant difference between three groups, in posttest1 and posttest 2 in terms of three variables (P < 0.0001). In addition, both licorice and green tea interventions had a high effect size on the decrease of the studied variables and there was no significant difference between these solutions (P < 0.0167).
Conclusion

Both green tea and licorice solutions greatly reduced post-extubation complications. These results can be clinical evidence for the use of these two plants to prevent complications of endotracheal extubation.
Trial registration:

This study was registered in the Iranian Registry of Clinical Trials (IRCT) with the code “IRCT20190729044373N1” on 2019-12-27.
Modeling the effect of distancing and wearing of face masks on transmission of Covid-19 infection dynamics
Authors: Lasisi Nurudeen O. , Adeyemo Kolawole A. 
Published: June 2021

The COVID-19 is an infection caused by corona virus which has been designated as pandemic in the word. In this paper, we proposed a model to study the effect of distancing and wearing of mask on transmission of Covid-19 infection dynamics. The invariant region of the model is established. The Covid-19 free equilibrium and the reproduction number of the model were obtained. The local and global stability of the model is determined using linearization technique method and Lyapunov method. It was found that, Covid-19 free equilibrium state is locally asymptotically stable in feasible region Ω if R0<1 and globally asymptomatically stable if R0<1, otherwise unstable if R0>1. More so, numerical analysis and simulations of the dynamics of the Covid-19 infection are presented. It was found that as effectiveness of face mask usage increased it resulted in a decrease in secondary cases and as physical distancing is maintained shown reduction in reproduction number. It is also found that, as infection rate increases, it translated to increase in secondary cases and as number of isolation individual increases, it decreased the reproduction number. More so, varying the treatment rate, means as treatment rate increases, it shown that an individuals in the isolation decreases.
Bioethics in Childbirth Care: Protocol for a Scoping Review
Authors: Ferreira Graziani Izidoro , Barbosa Kevin Haley , Duarte Andre Di Carlo , Oliveira Cesar De , Guilhem Dirce 
Published: June 2021

Background
Ensuring women’s rights during childbirth care based on humanized and bioethical principles results in better quality of care and patient safety and provides positive childbirth experiences.

Objective
We aim to explore the available evidence on the application of bioethical principles in the general context of childbirth care.

Methods
Our scoping review will follow the Joanna Briggs Institute Reviewer’s Manual. Published and unpublished bibliographic materials will be considered based on the following inclusion criteria: reports of the application of bioethical principles (concept) in assistance to the predelivery, childbirth, and postpartum periods (population) in the hospital context (context). We will search for relevant studies in PubMed and the Virtual Health Library, including MEDLINE, LILACS, BDENF, SCiELO, Web of Science, and Google Scholar. Two reviewers will perform the screening of titles and abstracts, read the full texts, and extract data from the selected articles. The data will then be organized and expressed into categories based on their content.

Results
The analyzed data will be presented through flowcharts, tables, and descriptive narratives. A paper summarizing the findings from this review will be published in a peer-reviewed journal. In addition, a synthesis of the key findings will be disseminated to health services linked to university hospitals in Brazil. They will also be shared with the academic community and policy makers involved in the Childbirth Assistance Network, which will potentially adopt our recommendations in their decision-making process regarding childbirth care practice in Brazil.

Conclusions
The findings from this review will inform, through the translation of knowledge, childbirth support groups, feminist movements, movements in favor of humanization of childbirth, and other childbirth support networks in the country.

Trial Registration
Open Science Framework; https://osf.io/kczyr/
Efficacy and Safety of Mineralocorticoid Receptor Antagonists in Kidney Failure Patients Treated with Dialysis: A Systematic Review and Meta-Analysis
Authors: Chen Kuan-Ting , Kang Yi-No , Lin Yen-Chung , Tsai I-Lin , Chang Wei-Chiao , Fang Te-Chao , Wu Mai-Szu , Kao Chih-Chin 
Published: June 2021

Background and objectives
Patients with kidney failure have a high risk of cardiovascular disease due to cardiac remodeling, left ventricular fibrosis, and hyperaldosteronism, all of which can be potentially mitigated by mineralocorticoid receptor antagonists. However, because of the fear of hyperkalemia, the use of mineralocorticoid receptor antagonists in patients with kidney failure is limited in current clinical practice, and few studies have investigated the efficacy and safety. Thus, we aimed to determine the benefits and side effects of mineralocorticoid receptor antagonists in patients with kidney failure treated with dialysis.

Design, setting, participants, & measurements
This is a systematic review and meta-analysis of randomized controlled trials published from 2005 to 2020 that compared the effect of mineralocorticoid receptor antagonists with either placebo or no treatment in patients with kidney failure. Two reviewers independently searched the PubMed, EMBASE, and Cochrane databases for all published studies, extracted data, assessed the risk of bias, and rated the quality of evidence. A meta-analysis was conducted on 14 eligible randomized controlled trials, and a total of 1309 patients were included.

Results
High-quality evidence suggested that mineralocorticoid receptor antagonists are associated with lower cardiovascular mortality (relative risk, 0.41; 95% confidence interval, 0.24 to 0.70; P=0.001) and all-cause mortality (relative risk, 0.44; 95% confidence interval, 0.30 to 0.66; P<0.001), and the risk of hyperkalemia was comparable with that of control group (relative risk, 1.12; 95% confidence interval, 0.91 to 1.36; P=0.29). However, no significant decrease in nonfatal cardiovascular events and stroke was observed, and there was no significant improvement in BP or cardiac performance parameters, including left ventricular ejection fraction and left ventricular mass index.

Conclusions
Our meta-analysis suggests that mineralocorticoid receptor antagonists might improve clinical outcomes of patients with kidney failure without significant increase in the risk of hyperkalemia.