bluuebunny/crossref_metadata_2025_split huggingface dataset:
Meningkatkan Motivasi Belajar Siswa Melalui Penerapan Model Discovery Learning Kelas VIII-A SMPN 2 Piani
Authors: Apriyadi Ahmad , Sukmawati R Ati 
Published: January 2021

Penelitian ini bertujuan untuk meningkatkan motivasi belajar dan hasil belajar siswa kelas VIII-A SMPN 2 Piani melalui model discovery learning. Penelitian ini merupakan Penelitian Tindakan Kelas (PTK). Siklus I terdiri dari dua kali pertemuan, dan siklus II terdiri dari tiga kali pertemuan. Subjek penelitian ini adalah siswa kelas VIII-A SMPN 2 Piani, yang terdiri dari 9 orang siswa laki-laki dan 11 orang siswa perempuan. Objek penelitan adalah motivasi belajar dan hasil belajar siswa dalam pembelajaran tekanan zat dengan menerapkan model discovery learning. Instrumen penelitian terdiri dari lembar observasi motivasi belajar siswa dan tes hasil belajar. Berdasarkan hasil penelitian diperoleh bahwa motivasi siswa dari siklus I dan siklus II mengalami peningkatan, dari kurang termotivasi menjadi termotivasi. Hasil Belajar siswa kelas VIII-A juga mengalami kenaikan ketuntasan belajar pada siklus I mencapai 65% selanjutnya pada siklus II menjadi 95% (melebihi batas ketuntasan klasikal= 80%). Sehingga model pembelajaran discovery learning dapat dijadikan salah satu cara untuk meningkatkan motivasi dan hasil belajar siswa.This study aims to improve learning motivation and student learning outcomes in class VIII-A at SMPN 2 Piani through a discovery learning model. This study is a classroom tidiness study. Cycles I are two encounters and cycles II are three encounters. The study was done by applying the discovery learning model. Action class consists of twenty pullings of research subjects made up nine male students and eleven female students. The object of research is the motivation to learn and the results of students degrees in the pressure of learning by applying the discovery learning model. The research instrument consisted of learning motivation sheets and learning achievement tests. Based on the research results, it was found that the motivation of students from cycle I and cycle II had increased, from less motivated to be motivated. Learning outcomes of class VIII-A students also experienced an increase in learning completeness in the first cycle reaching 65%; then in the second cycle, it became 95% (exceeding the classical completeness limit = 80%). So the discovery learning model can be a way to increase the motivation and learning result.
Pengembangan LKPD Berbasis STEM untuk Melatihkan Keterampilan Proses Sains Siswa Melalui Inkuiri Terbimbing
Authors: Mahjatia Nanda , Susilowati Eko , Miriam Sarah 
Published: January 2021

Penelitian ini dilatar belakangi oleh belum terlatihnya keterampilan proses sains peserta didik di salah satu sekolah di Kota Banjarmasin. Tujuan penelitian ini untuk mengembangkan Lembar Kerja Peserta didik (LKPD) berbasis STEM untuk melatihkan keterampilan proses sains dan mendeskripsikan kelayakan yang dilihat dari:1) Validitas LKPD; 2) Kepraktisan LKPD; 3) Efektivitas LKPD; 4) Pencapaian keterampilan proses sains; dan 5) Pencapaian STEM. Metode Penelitian dan Pengembangan digunakan dalam penelitian ini melalui tahap pengembangan model ADDIE dengan objek uji coba 22 peserta didik kelas XI MIPA 2 dan subjek LKPD berbasis STEM bertempat di salah satu sekolah di kota Banjarmasin. Hasil Penelitian ini menunjukkan: 1) Validitas LKPD berkategorikan valid. 2) LKPD yang dikembangkan dinyatakan praktis, karena angket respon dari peserta didik berkategorikan praktis. 3) Efektivitas LKPD yang dikembangkan diukur dari N-gain dimana nilai dari N-gain yang didapat berkategorikan sedang yang berarti efektif. 4) Pencapaian KPS melalui LKPD berbasis STEM yang didapat secara keseluruhan sangat baik dan mengalami peningkatan disetiap pertemuan. 5) Pencapaian STEM melalui LKPD berbasis STEM yang didapat secara keseluruhan sangat baik dan mengalami peningkatan disetiap pertemuan. Disimpulkan bahwa LKPD berbasis STEM untuk melatihkan keterampilan proses sains peserta didik layak digunakan dalam proses pembelajaran di tingkat SMA.This research was motivated by the untrained science process skills of students in a school in Banjarmasin City. The purpose of this study was to develop STEM-based student worksheets to train science process skills and describe the feasibility as seen from 1) the validity of the student worksheets; 2) practicality of student worksheets; 3) The effectiveness of student worksheets; 4) Achievement of science process skills; and 5) Achievement of STEM. The Research and Development method used in this study through the ADDIE model development stage with the test object of 22 students of class XI MIPA 2 and STEM-based student worksheets subjects located in one of the schools in the city Banjarmasin. The results of this study indicate: 1) The validity of student worksheets is categorized as valid. 2) The developed student worksheets were declared practical because the students' response questionnaire was categorized as practical. 3) The effectiveness of the developed student worksheets is measured from the N-gain where the N-gain value is categorized as a medium, which means effective. 4) The overall achievement of science process skills through STEM-based student worksheets is very good and has increased in every meeting. 5) Achievement of STEM through STEM-based student worksheets which is as a whole, is very good and has increased in every meeting. It was concluded that the STEM-based student worksheets to train students' science process skills was appropriate for use in the learning process at the high school level.
Pengembangan Bahan Ajar Model Quantum Teaching pada Materi Fluida Statis untuk Melatih Keterampilan Proses Sains Siswa
Authors: Ramadhan Taufik , Arifuddin Muhammad , Mastuang Mastuang 
Published: January 2021

Tujuan penelitian ini ialah menghasilkan produk pengembangan bahan ajar model quantum teaching pada materi fluida statis untuk melatih keterampilan proses sains siswa. Subjek untuk penelitian ini merupakan siswa kelas XI MIPA 2 di SMAN 12 Banjarmasin sebanyak 25 siswa. Penelitian ini merupakan penelitian pengembangan dengan menggunakan model pengembangan ADDIE. Pengumpulan data penelitian dilakukan dengan teknik observasi dan tes. Hasil dari penelitian menunjukkan bahwa 1) Hasil validasi bahan ajar memperoleh nilai rata-rata dalam kategori valid; 2) Kepraktisan bahan ajar berdasarkan keterlaksanaan rencana pelaksanaan pembelajaran memperoleh kategori sangat praktis; 3) Efefektivitas bahan ajar berdasarkan tes hasil belajar memperoleh kategori sedang; dan 4) Pencapaian keterampilan proses sains berkategori sangat baik. Simpulan dari penelitian ini bahwa bahan ajar model quantum teaching pada materi fluida statis layak digunakan untuk melatih keterampilan proses sains siswa.This research aims to produce a product, namely the quantum teaching model of teaching materials on static fluid material to train students' science process skills. The subjects for this research were 25 students of class XI MIPA 2 at SMAN 12 Banjarmasin. This research is development research using the ADDIE development model. Research data collection was carried out by observation and test techniques. The study results indicate that 1) The results of the validation of teaching materials obtain an average value in the valid category 2). The material's practicality based on the feasibility of the lesson plan implementation gets a very practical category 3). The effectiveness of teaching materials based on the learning result test obtained the medium category 4). The achievement of science process skills was categorized as very good. This research concludes that the quantum teaching model teaching materials on static fluid material are suitable for training students' science process skills.
Analysis of Expression Profiles of mRNA and lncRNA in Oviduct During Estrus Phase of Sheep (Ovis Aries) with Two Fecundity (FecB Gene) Genotypes
Authors: Chen Weihao , Li Zhifeng , Sun Wei , Chu Mingxing 
Published: January 2021

Abstract
Background:In sheep, FecB is the essential biomarker of the fertility, previous researches have provided a detailed insight on the regulation involved estrus phase and FecB in the reproductive-related tissues including hypothalamus, pituitary, and ovary. However, as the host of embryo development and connection between the ovary and the uterus, little is known about the interaction between mRNAs and lncRNAs in sheep oviduct. In the present study, RNA-Seq was performed to identify the transcriptomic profiles of mRNAs and lncRNAs in oviduct during estrus phase of sheep with FecBBB/++ genotypes.Results:In total, 21,863 lncRNAs and 43,674 mRNAs were identified, 57 DE lncRNAs and 637 DE mRNAs were revealed in the comparisons between follicular phase and luteal phase, 26 DE lncRNAs and 421 DE lncRNAs were revealed in the comparisons between FecB BB genotype and FecB ++ genotype. Functional enrichment analysis suggested that GO and KEGG terms related to reproduction such as SAGA complex, ATP-binding cassette (ABC), Nestin, and Hippo signalling pathway. DE-interaction network suggested that LNC_018420 maybe the key regulators related to embryo development in sheep oviduct.Conclusion:This was the first study to reveal the transcriptomic profiles of mRNAs and lncRNAs in the oviduct of FecB BB/++ sheep at estrus phase using RNA-Seq. Our findings can provide new understanding on the molecular mechanisms of mRNAs and lncRNAs underlying sheep embryo development and also opening new lines of investigation in sheep reproduction.
COVID-19 plasma deep proteome reveals distinct signatures in severe patients
Authors: Suvarna Kruthi , Biswas Deeptarup , Pai Medha Gayathri J , Acharjee Arup , Bankar Renuka , P. Viswanthram , Salkar Akanksha , Verma Ayushi , Mukherjee Amrita , Choudhury Manisha , Ghantasala Saicharan , Ghosh Susmita , Singh Avinash , Banerjee Arghya , Badaya Apoorva , Bihani Surbhi , Mantri Krishi , Burli Ananya , Roy Jyotirmoy , Srivast Alisha , Agrawal Sachee , Shrivastav Om , Shastri Jayanthi , Srivast Sanjeeva 
Published: January 2021

Abstract
Prognosis and management of COVID-19 severity is a challenge even after months of the pandemic. Host plasma proteome alterations carry insights into the physiological alterations in response to the infection. Here we employed a mass spectrometry-based label-free quantitative proteomics approach to study alteration in plasma proteome in a cohort of 73 patients (20 COVID negative, 18 non-severe, and 33 severe) to understand the disease dynamics for addressing this challenge. Of the 1200 proteins detected in the patient plasma, 38 proteins were differentially expressed between non-severe and severe groups. The host proteins such as Angiotensinogen, apolipoprotein B, SERPINA3, SERPING1, and Fibrinogen gamma chain identified in LFQ analysis were further validated using targeted mass spectrometry assay. Utilizing our proteomics dataset, we identified multiple drugs that could inhibit the upregulated proteins involved in disease pathogenesis of these 2 FDA-approved drugs Selinexor and Ponatinib, which showed promise of being re-purposed for potential therapeutics of COVID-19. Plasma proteome identified significant dysregulation in the pathways related to peptidase activity, regulated exocytosis, blood coagulation, complement activation, leukocyte activation involved in immune response, and response to glucocorticoid biological processes during severe SARS-CoV-2 infection. Further, the results suggest that COVID-19 severity can be prognosticated using specific biomarkers of severity, and few of these proteins are excellent targets for re-purposed drugs.
Effect of Metformin on the Epigenetic Age of Peripheral Blood in Patients With Diabetes Mellitus (DM)
Authors: Li Man , Duan Lei , Cai Yulun , Hao Benchuan , Chen Jianqiao , Liu Hongbin 
Published: January 2021

Abstract
Background: Metformin has been proved to have anti-aging effect. However, studies on how metformin affects global epigenetic regulation and its effect on the epigenetic clock in the diabetes mellitus (DM) patients is limited. This study aims to investigate the impact of metformin on the epigenetic age in subjects with type 2 DM.Methods: We collected the peripheral blood of metformin group and the no metformin group of the 32 DM patients. Epigenetic clock was used to estimate the epigenetic age acceleration of the two groups.Results: The results were presented as the following: 1) the metformin slow down the epigenetic age of the DM patients by an average of 2.77 years. 2) we observed a significant enrichment in the regulation of histone methyl-transferase activity (H3K9 specific) 3) we identified 144 differentially methylated positions (DMPs), the top 20 cytosine–guanine dinucleotides (CpGs) and their associate genes with the most consistent changes in the DNA methylation profile were selected: EPM2AIP1, MUC17, FNDC1, HLA-DOB, TRPC4, ESRRB, ADCY2. The major functions of most top-level differential methylation sites and the cellular signaling pathways they represent are related to aging process.Conclusions: Here we demonstrated that metformin could perform an anti-aging effect by slow down the epigenetic clock.
Burden of Oral Anticoagulation in Embolic Stroke of Undetermined Source without Atrial Fibrillation
Authors: Witte Klaus , Tsivgoulis Georgios , Reynolds Matthew , Tsintzos Stelios , Eggington Simon , Ismyrloglou Eleni , de Brouwer Bonnie , Lyon Julie , Huynh Marianne , Egea Marta , Ziegler Paul , Franco Noreli , Joglekar Rashmi , Rosemas Sarah , Liu Shufeng , Thijs Vincent 
Published: January 2021

Abstract
Objective: Prevention of recurrent stroke in patients with embolic stroke of undetermined source (ESUS) is challenging. The advent of safer anticoagulation in the form of direct oral anticoagulants (DOACs) has prompted exploration of prophylactic anticoagulation for all ESUS patients, rather than anticoagulating just those with documented atrial fibrillation (AF). However, recent trials have failed to demonstrate a clinical benefit, while observing increased bleeding. We modeled the economic impact of anticoagulating ESUS patients without documented AF across multiple geographies.Methods: CRYSTAL-AF trial data were used to assess ischaemic stroke event rates in ESUS patients confirmed AF-free after long-term monitoring. Anticipated bleeding event rates (including both minor and major bleeds) with aspirin, dabigatran 150 mg, and rivaroxaban 20 mg were sourced from published meta-analyses, whilst a 30% ischaemic stroke reduction for both DOACs was assumed. Cost data for clinical events and pharmaceuticals were collected from the local payer perspective.Results: Compared with aspirin, dabigatran and rivaroxaban resulted in 17.9 and 29.9 additional bleeding events per 100 patients over a patient’s lifetime, respectively. Despite incorporating into our model the proposed 30% reduction in ischaemic stroke risk, both DOACs were cost-additive over patient lifetime, as the costs of bleeding events and pharmaceuticals outweighed cost savings associated with the reduction in ischaemic strokes. DOACs added £5,953-£7,018 per patient (UK), €6,683-€7,368 (Netherlands), €4,933-€9,378 (Spain), AUD$5,353-6,539 (Australia) and $26,768-$32,259 (US) of payer cost depending on the agent prescribed. Additionally, in the U.S. patient pharmacy co-payments ranged from $1,716-$12,473 depending on agent and patient plan. In all settings, cost-savings could not be demonstrated even when the modelling assumed 100% protection from recurrent ischaemic strokes, due to the very low underlying risk of recurrent ischaemic stroke in this population (1.27 per 100 patient-years).Conclusions: Anticoagulation of non-AF patients may cause excess bleeds and add substantial costs for uncertain benefits, suggesting a personalised approach to anticoagulation in ESUS patients.
Deciphering intratumoral heterogeneity using integrated clonal tracking and single-cell transcriptome analyses
Authors: Lu Rong , CONTRERAS-TRUJILLO HUMBERTO , EERDENG JIYA , AKRE SAMIR , JIANG DU , JORAPUR APARNA , VERGEL-RODRIGUEZ MARY , ANDREASIAN AREEN , HARTON LISA , Bramlett Charles , GALA BASIA , Nogalska Anna , Chan Lai , Xiao Gang , LEE JAE-WOONG , MÜSCHEN MARKUS , MERCHANT AKIL 
Published: January 2021

Abstract
Cellular heterogeneity is a major cause of treatment resistance in cancer. Despite recent advances in single-cell genomic and transcriptomic sequencing, it remains difficult to relate measured molecular profiles to the cellular activities underlying cancer. Here, we present an integrated experimental system that connects single cell gene expression to heterogeneous cancer cell growth, metastasis, and treatment response. Our system integrates single cell transcriptome profiling with DNA barcode based clonal tracking in patient-derived xenograft models. We show that leukemia cells exhibiting unique gene expression signatures respond to different chemotherapies in distinct but consistent manners across multiple mice. In addition, we uncover an unexpected yet common form of leukemia expansion that is spatially confined to the bone marrow of single anatomical sites and driven by cells with distinct gene expression signatures. Our integrated system directly and effectively interrogates the molecular and cellular basis of the intratumoral heterogeneity underlying disease progression and treatment resistance.
Non-Invasive Plasma Glycomic and Metabolic Biomarkers of Post-treatment Control of HIV
Authors: Giron Leila , Palmer Clovis , Liu Qin , Yin Xiangfan , Papasavvas Emmanouil , Damra Mohammad , Goldman Aaron , Tang Hsin-Yao , Johnston Rowena , Mounzer Karam Mounzer , Kostman Jay , Tebas Pablo , Landay Alan , Montaner Luis , Jacobson Jeffrey , Li Jonathan , Abdel-Mohsen Mohamed 
Published: January 2021

Abstract
Non-invasive biomarkers that predict HIV remission after antiretroviral therapy (ART) interruption are urgently needed. Such biomarkers can improve the safety of analytic treatment interruption (ATI) and provide mechanistic insights into the pathways involved in post-ART HIV control. We identified plasma glycomic and metabolic signatures of time-to-viral-rebound and probability-of-viral-rebound using samples from two independent cohorts. These samples include a large number of post-treatment controllers, a rare population demonstrating sustained virologic suppression after ART-cessation. The signatures remained significant after adjusting for key demographic and clinical confounders. We also confirmed a mechanistic link between biomarkers and HIV latency reactivation and myeloid inflammation in vitro. Finally, machine learning algorithms selected sets of biomarkers that predict time-to-viral-rebound with 74-76% capacity and probability-of-viral-rebound with 97.5% capacity. In summary, we fill a major gap in HIV cure research by identifying non-invasive biomarkers, with potential functional significance, that predict duration and probability of viral remission after treatment interruption.
Impact of Lenvatinib on Renal Function: Long-term Analysis of Differentiated Thyroid Cancer Patients
Authors: Masaki Chie , Sugino Kiminori , Kobayashi Sakiko , Hosoi Yoshie , Ono Reiko , Yamazaki Haruhiko , Akaishi Junko , Hames Kiyomi , Tomoda Chisato , Suzuki Akifumi , Matsuzu Kenichi , Ohkuwa Keiko , Kitagawa Wataru , Nagahama Mitsuji , Ito Koichi 
Published: January 2021

Abstract
Background: Because lenvatinib is well known to induce proteinuria by blocking the vascular endothelial growth factor (VEGF) pathway, renal function is a concern for patients with long-term administration of lenvatinib. The long-term effects of lenvatinib on renal function in patients with advanced differentiated thyroid carcinoma (DTC) were analyzed. Method: This study involved 40 DTC patients who continued lenvatinib therapy for ≥6 months. Estimated glomerular filtration rate (eGFR) was calculated as an indicator of renal function. The temporal course of eGFR, effects of baseline eGFR on eGFR changes, and factors affecting renal impairment were investigated. Results: The overall cohort showed sustainable decreases in eGFR, with decreased values of 11.4, 18.3, and 21.0 mL/min/1.73 m2 at 24, 36, and 48 months after starting treatment, respectively. No differences in eGFR decrease every 6 months were seen for three groups classified by baseline eGFR ≥90 mL/min/1.73 m2 (n=6), ≥60 by <90 mL/min/1.73 m2 (n=26), and ≥45 but <60 mL/min/1.73 m2 (n=8). Grade 3 proteinuria was associated with decline in eGFR (p=0.0283). Long observation period was also associated with a decrease in eGFR (p=0.0115), indicating that eGFR may decrease in a time-dependent manner. Conclusion: Lenvatinib can induce decline in eGFR, especially with treatment duration >2 years, regardless of baseline eGFR. Proteinuria is a risk factor for decline in eGFR. Patients who start lenvatinib with better renal function show a renal reserve capacity, prolonging clinical outcomes. Decision-making must balance the benefits of lenvatinib continuation with allowed harm.
Normal reference intervals of prognostic nutritional index in healthy adults: A large multi-center observational study from Western China
Authors: Yang Guishu , Wang Dongsheng , Zu Ruiling , He Linbo , Zhang Guangjie , Yu Jianhong , Chen Yaping , Yin Hailin , Liao Yulin , Li Shi , Deng Yao , Zhang Kaijiong , Li Tian , Zhu Guiquan , Luo Huaichao 
Published: January 2021

Abstract
Background

It has been widely reported that the prognostic nutritional index (PNI) played a pivotal role in nutritional assessment of surgical patients and tumor prognosis. In order to improve the accuracy of evaluation in western China, we established reference intervals (RIs) of PNI in healthy controls.
Methods

A retrospective cohort study on healthy ethnic Han adults (18–79 years) was conducted to explore the influences of age, gender, study centers, and instruments on PNI and to establish RIs. The data came from a healthy routine examination center database and laboratory information system (LIS) of four centers in western China, and there were 200 persons selected randomly for verification of RIs.
Results

5,839 healthy candidates were enrolled. PNI showed a marked gender dependence, and males had significantly higher PNI than females across all ages (P < 0.01). We found that PNI is significantly different between age groups (P < 0.01), the value of PNI tended to decrease with age increasing. There is also an obvious influence of centers and instruments on PNI (P < 0.01).
Conclusions

We established reference intervals of PNI in healthy Han Chinese population in Western China, and validated successfully. Further established RIs will lead to better standardizations of PNI for clinical applications.