bluuebunny/crossref_metadata_2025_split huggingface dataset:
Real-time locating systems to improve healthcare delivery: A systematic review
Authors: Overmann Kevin M , Wu Danny T.Y , Xu Catherine T , Bindhu Shwetha S , Barrick Lindsey 
Published: February 2021

Abstract

Objective
Modern health care requires patients, staff, and equipment to navigate complex environments to deliver quality care efficiently. Real-time locating systems (RTLS) are local tracking systems that identify the physical locations of personnel and equipment in real time. Applications and analytic strategies to utilize RTLS-produced data are still under development. The objectives of this systematic review were to describe and analyze the key features of RTLS applications and demonstrate their potential to improve care delivery.

Materials and Methods
We searched MEDLINE, SCOPUS, and IEEE following PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-Analyses) guidelines. Inclusion criteria were articles that utilize RTLS to evaluate or influence workflow in a healthcare setting. We summarized aspects of relevant articles, identified key themes in the challenges of applying RTLS to workflow improvement, and thematically reviewed the state of quantitative analytic methodologies.

Results
We included 42 articles in the final qualitative synthesis. The most frequent study design was observational (n = 24), followed by descriptive (n = 12) and experimental (n = 6). The most common clinical environment for study was the emergency department (n = 12), followed by entire hospital (n = 7) and surgical ward (n = 6).

Discussion
The focus of studies changed over time from early experience to optimization to evaluation of an established system. Common narrative themes highlighted lessons learned regarding evaluation, implementation, and information visibility. Few studies have developed quantitative techniques to effectively analyze RTLS data.

Conclusions
RTLS is a useful and effective adjunct methodology in process and quality improvement, workflow analysis, and patient safety. Future directions should focus on developing enhanced analysis to meaningfully interpret RTLS data.
Overall Health Care Cost During the Year Following Diagnosis of Colorectal Cancer Stratified by History of Colorectal Evaluative Procedures
Authors: Paszat Lawrence , Sutradhar Rinku , Luo Jin , Rabeneck Linda , Tinmouth Jill , Baxter Nancy N 
Published: February 2021

Abstract

Background
The cost-effectiveness of colorectal screening has been modeled; however, the cost of health care following the diagnosis of colorectal cancer has not been described stratified by history of colorectal evaluative procedures.

Methods
We identified persons with first diagnosis of colorectal cancer between 2015 and 2017 from the Ontario Cancer Registry, and categorized them by history of colorectal evaluative procedures during Period 1 (the 10 years before the 6-month prediagnostic interval) with or without procedures during Period 2 (the 6 month prediagnostic interval), versus only during Period 2, versus none. We extracted overall health care cost 1 year following diagnosis from population-wide administrative databases.

Results
Among cases diagnosed at 52 to 74 years, overall health care cost among those with no colorectal evaluative procedures on or before the date of diagnosis is $71,039.65 (SD $51,825.18), compared to $48,406.15 (SD $38,843.64) among those who received colorectal evaluative procedures during Period 1, with or without procedures during Period 2. Among the population aged 20 to 74 years at diagnosis, cases with ≥1 screening colonoscopies for hereditary CRC syndrome, the mean overall initial cost was between $32,300.32 (SD) and $33,084.67 (SD $39,905.77), and those with ≥1 screening colonoscopies because of a first-degree relative with CRC, was between $36,344.71 (SD $35,539.85) and $45,456.41 (SD $49,818.59).

Conclusions
Overall health care cost is lower among cases who received colorectal evaluative procedures during Period 1, with or without procedures during Period 2, and among those with screening colonoscopy for hereditary CRC syndromes or affected first-degree relatives.
A Novel Methodology for Supporting Integration between Refugees and Host Communities: NAUTIA (Need Assessment under a Technological Interdisciplinary Approach)
Authors: Salas-Ruiz Adela , Eras-Almeida Andrea A , Rodríguez-Rivero Rocío , Sanz-Cobena Alberto , MuÑoz-Hernández Susana , Canet Juana , Rojo-Losada Alejandra , Gesto-Barroso Belén 
Published: February 2021

Abstract
More than 26 million people are recognized globally as refugees and have been forced to flee from their home countries because of poverty, human rights violations, natural disasters, climate change, and other social and political conflicts. What is more, most host communities are usually poor and face social and economic crises. This is why supporting integration between refugees and host communities is imperative at the global humanitarian context. Thereby, this research presents the NAUTIA (Need Assessment under a Technological Interdisciplinary Approach) methodology, an innovative mixed-method approach designed by the Platform on Refugees of the Universidad Politécnica de Madrid. The main objective of NAUTIA is to identify the basic needs of refugees and locals to improve their quality of life through interdisciplinary and inclusive intervention proposals based on technology. The methodology was applied in the permanent Shimelba Refugee Camp (Ethiopia), where energy, shelter, and food security solutions have resulted essential to improve the living conditions of both population groups. The results are useful for researchers, stakeholders, and practitioners from the humanitarian sector as they provide a more innovative and comprehensive way to support the unprecedented global human mobility there is nowadays.
A fruit ripening-associated transcription factor CsMADS5 positively regulates carotenoid biosynthesis in citrus
Authors: Lu Suwen , Ye Junli , Zhu Kaijie , Zhang Yin , Zhang Mengwei , Xu Qiang , Deng Xiuxin 
Published: February 2021

AbstractCarotenoids in citrus contribute to the quality of the fruit, but the mechanism of its transcriptional regulation is fairly unknown. Here, we characterized a citrus FRUITFULL sub-clade MADS gene, CsMADS5, that was ripening-inducible and acted as a nucleus-localized trans-activator. Transient overexpression of CsMADS5 in citrus induced fruit coloration and enhanced carotenoid concentrations. The expression of carotenogenic genes including phytoene synthase (PSY), phytoene desaturase (PDS), and lycopene β-cyclase 1 (LCYb1) was increased in the peels of fruits overexpressing CsMADS5. Similar results were observed from stable overexpression of CsMADS5 in tomato fruits and citrus calli, even though the effect of CsMADS5 on carotenoid metabolism in transgenic citrus calli was limited. Further biochemical analyses demonstrated that CsMADS5 activated the transcription of PSY, PDS, and LCYb1 by directly binding to their promoters. We concluded that CsMADS5 positively regulates carotenoid biosynthesis in fruits by directly activating the transcription of carotenogenic genes. Moreover, CsMADS5 physically interacted with a positive regulator CsMADS6, indicating that CsMADS5 may form an enhancer complex with CsMADS6 to synergistically promote carotenoid accumulation. These findings expand our understanding of the complex transcriptional regulatory hierarchy of carotenoid biosynthesis during fruit ripening.
Outgrowth of the axillary bud in rose is controlled by sugar metabolism and signalling
Authors: Wang Ming , Pérez-Garcia Maria-Dolores , Davière Jean-Michel , Barbier François , Ogé Laurent , Gentilhomme José , Voisine Linda , Péron Thomas , Launay-Avon Alexandra , Clément Gilles , Baumberger Nicolas , Balzergue Sandrine , Macherel David , Grappin Philippe , Bertheloot Jessica , Achard Patrick , Hamama Latifa , Sakr Soulaiman 
Published: February 2021

AbstractShoot branching is a pivotal process during plant growth and development, and is antagonistically orchestrated by auxin and sugars. In contrast to extensive investigations on hormonal regulatory networks, our current knowledge on the role of sugar signalling pathways in bud outgrowth is scarce. Based on a comprehensive stepwise strategy, we investigated the role of glycolysis/the tricarboxylic acid (TCA) cycle and the oxidative pentose phosphate pathway (OPPP) in the control of bud outgrowth. We demonstrated that these pathways are necessary for bud outgrowth promotion upon plant decapitation and in response to sugar availability. They are also targets of the antagonistic crosstalk between auxin and sugar availability. The two pathways act synergistically to down-regulate the expression of BRC1, a conserved inhibitor of shoot branching. Using Rosa calluses stably transformed with GFP-fused promoter sequences of RhBRC1 (pRhBRC1), glycolysis/TCA cycle and the OPPP were found to repress the transcriptional activity of pRhBRC1 cooperatively. Glycolysis/TCA cycle- and OPPP-dependent regulations involve the –1973/–1611 bp and –1206/–709 bp regions of pRhBRC1, respectively. Our findings indicate that glycolysis/TCA cycle and the OPPP are integrative parts of shoot branching control and can link endogenous factors to the developmental programme of bud outgrowth, likely through two distinct mechanisms.
TALE-triggered and iTALE-suppressed Xa1-mediated resistance to bacterial blight is independent of rice transcription factor subunits OsTFIIAγ1 or OsTFIIAγ5
Authors: Xu Xiameng , Xu Zhengyin , Ma Wenxiu , Haq Fazal , Li Ying , Shah Syed Mashab Ali , Zhu Bo , Zhu Changxiang , Zou Lifang , Chen Gongyou 
Published: February 2021

Abstract
Xa1-mediated resistance to rice bacterial blight, caused by Xanthomonas oryzae pv. oryzae (Xoo), is triggered by transcription activator-like effectors (TALEs) and suppressed by interfering TALEs (iTALEs). TALEs interact with the rice transcription factor OsTFIIAγ1 or OsTFIIAγ5 (Xa5) to activate expression of target resistance and/or susceptibility genes. However, it is not clear whether OsTFIIAγ is involved in TALE-triggered and iTALE-suppressed Xa1-mediated resistance. In this study, genome-edited mutations in OsTFIIAγ5 or OsTFIIAγ1 of Xa1-containing rice ‘IRBB1’ and Xa1-transgenic plants of xa5-containing rice ‘IRBB5’ did not impair the activation or suppression of Xa1-mediated resistance. Correspondingly, the expression pattern of Xa1 in mutated OsTFIIAγ5 and OsTFIIAγ1 rice lines and ‘IRBB1’ rice was similar. In contrast, the expression of OsSWEET11 was repressed in rice lines mutated in OsTFIIAγ5 and OsTFIIAγ1. Bimolecular fluorescence complementation (BiFC) and co-immunoprecipitation assays showed that both TALE PthXo1 and iTALE Tal3a interacted with OsTFIIAγ1 and OsTFIIAγ5 in plant nuclei. These results indicated that TALE-triggered and iTALE-suppressed Xa1-mediated resistance to bacterial blight is independent of OsTFIIAγ1 or OsTFIIAγ5 in rice, and suggest that an unknown factor is potentially involved in the interaction of Xa1, TALEs and iTALEs.
Express Rights of Termination
Authors: Stannard John E , Capper David 
Published: February 2021

Abstract
This chapter discusses express rights of termination. It is not always easy for a party to know for certain whether they are entitled to terminate or not. This is particularly so where the right in question depends on proof of fundamental breach or repudiation. For this reason, parties to a contract frequently, in the interests of certainty, make express provision for this by agreeing in advance that one or both of them may terminate if certain conditions are met. Such express rights of termination can depend on a wide variety of contingencies, but very frequently these will include a breach by the other party. Where this is the case, it is often difficult to distinguish termination under the express right from termination under the general law, particularly where the latter involves termination for breach of condition. The chapter then assesses four key issues with regard to express rights of this sort, most notably: (1) the relationship between express rights of termination and conditions at common law; (2) the requirements for the exercise of such rights; (3) the effect of termination under such a right; and (4) the problem of concurrent rights of termination.
ITLOS and the Dispute Settlement System under UNCLOS
Authors: Kittichaisaree Kriangsak 
Published: February 2021

Abstract
This chapter discusses the dispute settlement system under the 1982 United Nations Convention on the Law of the Sea (UNCLOS). The dispute settlement system under UNCLOS was drafted with the main purpose of achieving the uniform and effective interpretation and application of UNCLOS, as the compromises it embodied would otherwise be vulnerable to unilateral interpretation. UNCLOS sets up two international organizations: the International Seabed Authority (Authority) and the International Tribunal for the Law of the Sea (ITLOS). The Authority is the organization through which States Parties to UNCLOS organize and control activities in the seabed and ocean floor and subsoil thereof, beyond the limits of national jurisdiction (the Area), particularly with a view to administering the resources of the Area. ITLOS, the dispute settlement mechanism specifically created by UNCLOS, allows urgent issues to be tackled swiftly through seeking provisional measures of protection from a court or tribunal as well as a functional approach. The chapter then considers ITLOS's place within the dispute settlement regime under UNCLOS; entities with access to ITLOS; other international agreements besides UNCLOS that confer jurisdiction on ITLOS; ITLOS's relationship and interaction with the other principal choices of dispute settlement mechanisms; and the pros and cons of using ITLOS instead of other forums.
Overview of ITLOS’s Composition, Organization, Structure, and Rules of Procedure
Authors: Kittichaisaree Kriangsak 
Published: February 2021

Abstract
This chapter provides an overview of the composition, organization, structure, and rules of procedure of the International Tribunal for the Law of the Sea (ITLOS). ITLOS is composed of 21 judges elected by secret ballot by the States Parties to the 1982 United Nations Convention on the Law of the Sea (UNCLOS) from among persons nominated by States Parties who enjoy the highest reputation for fairness and integrity and of recognized competence in the field of the law of the sea. The United Nations recognizes ITLOS as an autonomous international judicial body with jurisdiction as provided for in the relevant provisions of UNCLOS and its Statute (which is Annex VI to UNCLOS). The UN and ITLOS each undertakes to respect the status and mandate of the other and to establish cooperative working relations pursuant to the provisions of the Agreement on Cooperation and Relationship between the UN and ITLOS. With a view to facilitating the effective attainment of their objectives and the coordination of their activities, they shall consult and cooperate, whenever appropriate, on matters of mutual concern, and pursue, whenever appropriate, initiatives to coordinate their activities. Moreover, ITLOS's Registrar transmits to the UN information and documentation relating to ITLOS's work, including documentation relating to applications, pleadings, oral proceedings, orders, judgments, and other communications and documentation before ITLOS.
Applications for Provisional Measures
Authors: Kittichaisaree Kriangsak 
Published: February 2021

Abstract
This chapter assesses applications for provisional measures of protection under the 1982 United Nations Convention on the Law of the Sea (UNCLOS). At the Third UN Conference on the Law of the Sea, the need for courts or tribunals having jurisdiction under UNCLOS to have the power to prescribe provisional measures was beyond dispute although there was considerable debate concerning the details of the regime associated with such measures. The finally adopted Article 290 of UNCLOS, under the heading ‘Provisional measures’, represents the best possible compromise. Provisional measures are divided into provisional measures prescribed by the International Tribunal for the Law of the Sea (ITLOS) under Article 290(1) pending ITLOS’ judgment on the merits of the dispute, on the one hand, and provisional measures prescribed by ITLOS under Article 290(5) pending the constitution of an arbitral tribunal to which a dispute is being submitted, on the other hand. The request for the prescription of provisional measures shall be in writing and specify the measures requested, the reasons therefor, and the possible consequences, if the request is not granted, for the preservation of the respective rights of the parties or for the prevention of serious harm to the marine environment.
Good Deaths in Wendell Berry’s Short Stories
Authors: Ens Gerald 
Published: February 2021

Abstract
This article looks at how Wendell Berry’s short stories depicting good deaths offer a crucial exploration of the incarnate bonds of human affection. They do so, I argue, by pointing us to the vulnerable ordinariness of embodied love. I first describe these good deaths as ‘ordinary’ because of the way that they refuse a heroic mode of standing above the world and instead accept and live into the vulnerable connections that mark our materiality. I show also how this acceptance, and not any attempt to transcend the ordinary, is what opens these deaths up to the sacred, which I argue is a mark of belonging in love to the world and the love that moves the world. In the second section, I outline the relational role death plays in inaugurating and sustaining the gift-giving relational bonds that make up the life of affection in a place, such that there is a sense in which it is death that opens us up to love, even as death always marks an absence.
The Genomes of Two Billfishes Provide Insights into the Evolution of Endothermy in Teleosts
Authors: Wu Baosheng , Feng Chenguang , Zhu Chenglong , Xu Wenjie , Yuan Yuan , Hu Mingliang , Yuan Ke , Li Yongxin , Ren Yandong , Zhou Yang , Jiang Haifeng , Qiu Qiang , Wang Wen , He Shunping , Wang Kun 
Published: February 2021

AbstractEndothermy is a typical convergent phenomenon which has evolved independently at least eight times in vertebrates, and is of significant advantage to organisms in extending their niches. However, how vertebrates other than mammals or birds, especially teleosts, achieve endothermy has not previously been fully understood. In this study, we sequenced the genomes of two billfishes (swordfish and sailfish), members of a representative lineage of endothermic teleosts. Convergent amino acid replacements were observed in proteins related to heat production and the visual system in two endothermic teleost lineages, billfishes and tunas. The billfish-specific genetic innovations were found to be associated with heat exchange, thermoregulation, and the specialized morphology, including elongated bill, enlarged dorsal fin in sailfish and loss of the pelvic fin in swordfish.
Endometrial stromal cell proteomic analysis reveals LIM and SH3 protein 1 (LASP1) plays important roles in the progression of adenomyosis
Authors: Liu Faying , Li Zengming , Guo Jiubai , Fang Shufen , Zhou Jiangyan , Cao Bianna , Liu Jun , Yi Yulan , Yuan Xiaoqun , Xu Xiaoyun , Huang Ouping , Wang Liqun , Zou Yang 
Published: February 2021

Abstract
Adenomyosis is one of the most common gynecological disorders that the molecular events underlying its pathogenesis remain not fully understood. Prior studies have shown that endometrial stromal cells (ESCs) played crucial roles in the pathogenesis of adenomyosis. In this study, we utilized two-dimensional gel electrophoresis combined with protein identification by mass spectrometry (2D/MS) proteomics analysis to compare the differential protein expression profile between the paired eutopic and ectopic ESCs (EuESCs and EcESCs) in adenomyosis, and a total of 32 significantly altered protein spots were identified. Among which, the expression of LIM and SH3 protein 1 (LASP1) was increased significantly in EcESCs compared to EuESCs. Immunohistochemical assay showed that LASP1 was overexpressed in the stromal cells of ectopic endometriums compared to eutopic endometriums; further functional analyses revealed that LASP1 overexpression could enhance cell proliferation, migration and invasion of EcESCs. Furthermore, we also showed that the dysregulated expression of LASP1 in EcESCs was associated with DNA hypermethylation in the promoter region of the LASP1 gene. However, the detailed molecular mechanisms of enhancing cell proliferation, invasion and migration caused by upregulated LASP1 in adenomyosis needs further study. For the first time, our data suggested that LASP1 plays important roles in the pathogenesis of adenomyosis, and could serve as a prognostic biomarker of adenomyosis.
Site-specific incorporation of 5′-methyl DNA enhances the therapeutic profile of gapmer ASOs
Authors: Vasquez Guillermo , Freestone Graeme C , Wan W Brad , Low Audrey , De Hoyos Cheryl Li , Yu Jinghua , Prakash Thazha P , Ǿstergaard Michael E , Liang Xue-hai , Crooke Stanley T , Swayze Eric E , Migawa Michael T , Seth Punit P 
Published: February 2021

Abstract
We recently showed that site-specific incorporation of 2′-modifications or neutral linkages in the oligo-deoxynucleotide gap region of toxic phosphorothioate (PS) gapmer ASOs can enhance therapeutic index and safety. In this manuscript, we determined if introducing substitution at the 5′-position of deoxynucleotide monomers in the gap can also enhance therapeutic index. Introducing R- or S-configured 5′-Me DNA at positions 3 and 4 in the oligodeoxynucleotide gap enhanced the therapeutic profile of the modified ASOs suggesting a different positional preference as compared to the 2′-OMe gap modification strategy. The generality of these observations was demonstrated by evaluating R-5′-Me and R-5′-Ethyl DNA modifications in multiple ASOs targeting HDAC2, FXI and Dynamin2 mRNA in the liver. The current work adds to a growing body of evidence that small structural changes can modulate the therapeutic properties of PS ASOs and ushers a new era of chemical optimization with a focus on enhancing the therapeutic profile as opposed to nuclease stability, RNA-affinity and pharmacokinetic properties. The 5′-methyl DNA modified ASOs exhibited excellent safety and antisense activity in mice highlighting the therapeutic potential of this class of nucleic acid analogs for next generation ASO designs.
Mechanism of forkhead transcription factors binding to a novel palindromic DNA site
Authors: Li Jun , Dai Shuyan , Chen Xiaojuan , Liang Xujun , Qu Lingzhi , Jiang Longying , Guo Ming , Zhou Zhan , Wei Hudie , Zhang Huajun , Chen Zhuchu , Chen Lin , Chen Yongheng 
Published: February 2021

AbstractForkhead transcription factors bind a canonical consensus DNA motif, RYAAAYA (R = A/G, Y = C/T), as a monomer. However, the molecular mechanisms by which forkhead transcription factors bind DNA as a dimer are not well understood. In this study, we show that FOXO1 recognizes a palindromic DNA element DIV2, and mediates transcriptional regulation. The crystal structure of FOXO1/DIV2 reveals that the FOXO1 DNA binding domain (DBD) binds the DIV2 site as a homodimer. The wing1 region of FOXO1 mediates the dimerization, which enhances FOXO1 DNA binding affinity and complex stability. Further biochemical assays show that FOXO3, FOXM1 and FOXI1 also bind the DIV2 site as homodimer, while FOXC2 can only bind this site as a monomer. Our structural, biochemical and bioinformatics analyses not only provide a novel mechanism by which FOXO1 binds DNA as a homodimer, but also shed light on the target selection of forkhead transcription factors.