bluuebunny/crossref_metadata_2025_split huggingface dataset:
Phenotype and synthesis activity of joint chondrocytes extracted from newborn rats with prenatal ethanol exposure
Authors: Cardoso Kênia Mara M C , Gomes Lorenna A , Reis Amanda Maria S , Silva Carla Maria O , Tamiasso Natalia V , Serakides Rogéria , Ocarino Natalia M 
Published: September 2021

Thirteen female Wistar rats were divided into two groups: one treated with ethanol and the other of untreated. Four newborns from each mother were selected and weighed, measured, and evaluated for physical characteristics. From these neonates, chondrocytes were extracted from the articular cartilages of the femur and tibia, and cultivated in a chondrogenic medium at 37oC and 5% CO2. At 7, 14, and 21 days of cultivation, alkaline phosphatase activity tests, MTT conversion to formazan, and percentage area covered by cells per field were performed. At 21 days, the percentage of PAS+ areas in 3D cultures was performed, as well as the evaluation of gene transcript expression for aggrecan, SOX-9, collagen type II, collagen X, Runx-2, and VEGF by real-time RT-PCR. The means were compared by Student’s t-test. The weight of the ethanol group neonates was significantly lower than that of the controls. Chondrocyte cultures from the ethanol group showed significantly higher AP activity, MTT conversion, and cell percentage. There was higher expression of collagen type II and lower expression of SOX-9 in the ethanol group. There was no difference in the percentage of PAS+ areas in pellets and in expression of aggrecan, collagen X, Runx-2, or VEGF between groups. In conclusion, prenatal exposure to ethanol alters the phenotype and activity of offspring chondrocytes, which may be mechanisms by which endochondral bone formation is compromised by maternal ethanol consumption.
Establishment and Validation of a Prognostic Immune Signature in Neuroblastoma
Authors: Yu Yunhu , Zeng Yu , Xia Xiangping , Zhou Jian-Guo , Cao Fang 
Published: September 2021

Background Neuroblastoma (NBL) is the most common extracranial solid tumor in childhood, and patients with high-risk neuroblastoma had a relatively poor prognosis despite multimodal treatment. To improve immunotherapy efficacy in neuroblastoma, systematic profiling of the immune landscape in neuroblastoma is an urgent need. Methods RNA-seq and according clinical information of neuroblastoma were downloaded from the TARGET database and GEO database (GSE62564). With an immune-related-gene set obtained from the ImmPort database, Immune-related Prognostic Gene Pairs for Neuroblastoma (IPGPN) for overall survival (OS) were established with the TARGET-NBL cohort and then verified with the GEO-NBL cohort. Immune cell infiltration analysis was subsequently performed. The integrated model was established with IPGPN and clinicopathological parameters. Immune cell infiltration was analyzed with the XCELL algorithm. Functional enrichment analysis was performed with clusterProfiler package in R. Results Immune-related Prognostic Gene Pairs for Neuroblastoma was successfully established with seven immune-related gene pairs (IGPs) involving 13 unique genes in the training cohort. In the training cohort, IPGPN successfully stratified neuroblastoma patients into a high and low immune-risk groups with different OS (HR=3.92, P = 2 × 10−8) and event-free survival (HR=3.66, P=2 × 10−8). ROC curve analysis confirmed its predictive power. Consistently, high IPGPN also predicted worse OS (HR=1.84, P = .002) and EFS in validation cohort (HR=1.38, P = .06) Moreover, higher activated dendritic cells, M1 macrophage, Th1 CD4+, and Th2 CD4+ T cell enrichment were evident in low immune-risk group. Further integrating IPGPN with age and stage demonstrated improved predictive performance than IPGPN alone. Conclusion Herein, we presented an immune landscape with IPGPN for prognosis prediction in neuroblastoma, which complements the present understanding of the immune signature in neuroblastoma.
Effect of Helicobacter Pylori on Plasma Metabolic Phenotype in Patients With Gastric Cancer
Authors: Wang Yan-Ping , Wei Ting , Ma Xiao , Zhu Xiao-Liang , Ren Long-Fei , Zhang Lei , Ding Fang-Hui , Li Xun , Wang Hai-Ping , Bai Zhong-Tian , Zhu Ke-Xiang , Miao Long , Yan Jun , Zhou Wen-Ce , Meng Wen-Bo , Liu Yu-Qin 
Published: September 2021

Background Although Helicobacter pylori (Hp) as high risk factor for gastric cancer have been investigated from human trial, present data is inadequate to explain the effect of Hp on the changes of metabolic phenotype of gastric cancer in different stages. Purpose Herein, plasma of human superficial gastritis (Hp negative and positive), early gastric cancer and advanced gastric cancer analyzed by UPLC-HDMS metabolomics can not only reveal metabolic phenotype changes in patients with gastric cancer of different degrees (30 Hp negative, 30 Hp positive, 20 early gastric cancer patients, and 10 advanced gastric cancer patients), but also auxiliarily diagnose gastric cancer. Results Combined with multivariate statistical analysis, the results represented biomarkers different from Hp negative, Hp positive, and the alterations of metabolic phenotype of gastric cancer patients. Forty-three metabolites are involved in amino acid metabolism, and lipid and fatty acid metabolism pathways in the process of cancer occurrence, especially 2 biomarkers glycerophosphocholine and neopterin, were screened in this study. Neopterin was consistently increased with gastric cancer progression and glycerophosphocholine tended to consistently decrease from Hp negative to advanced gastric cancer. Conclusion This method could be used for the development of rapid targeted methods for biomarker identification and a potential diagnosis of gastric cancer.
Current Trends of Practices in Nonclinical Toxicology: An Industry Survey
Authors: Authier Simon , Brock William J , Halpern Wendy , Harris Stephanie N , Jones David , McGovern Timothy , McGovern Pamela D , Pugsley Michael K 
Published: September 2021

The growth in drug development over the past years reflects significant advancements in basic sciences and a greater understanding of molecular pathways of disease. Benchmarking industry practices has been important to enable a critical reflection on the path to evolve pharmaceutical testing, and the outcome of past industry surveys has had some impact on best practices in testing. A survey was provided to members of SPS, ACT, and STP. The survey consisted of 37 questions and was provided to 2550 participants with a response rate of 24%. Most respondents (∼75%) came from the US and Europe. The survey encompassed multiple topics encountered in nonclinical testing of pharmaceuticals. The most frequent target indications were oncology (69%), inflammation (55%), neurology/psychiatry/pain (46%), cardiovascular (44%), and metabolic diseases (39%). The most frequent drug-induced toxicology issues confronted were hepatic, hematopoietic, and gastrointestinal. Toxicological effects that impacted the no observed adverse effect level (NOAEL) were most frequently based on histopathology findings. The survey comprised topics encountered in the use of biomarkers in nonclinical safety assessment, most commonly those used to assess inflammation, cardiac/vascular, renal, and hepatic toxicity as well as common practices related to the assessment of endocrine effects, carcinogenicity, genotoxicity, juvenile and male-mediated developmental and female reproductive toxicity. The survey explored the impact of regulatory meetings on program design, application of the 3 Rs, and reasons for program delays. Overall, the survey results provide a broad perspective of current practices based on the experience of the scientific community engaged in nonclinical safety assessment.
A new deck arch bridge and a study on its mechanical properties by FE and experiment methods
Authors: Qin Xia , Pang Mulin , Xie Xiaoli , Yan Chenyu , Liang Mingzhe 
Published: September 2021

In this study, a new type of deck arch bridge was proposed based on the triangle stability principle, and its mechanical properties were tested. The new deck arch bridge—named the superposed truss arch bridge—consists of main arches, auxiliary arches, a girder, and web members. These components adopt steel structures and are connected into a series of triangles to form a superposed truss structure. The new structural system design retains the advantages of the truss and arch structure. Additionally, the rise-span ratio of the main arch can be smaller in the new system design, so the rise height is smaller, which can decrease construction difficulty. The underlying mechanical principles of the new bridge were explained. A new type of railway deck arch bridge with a 650 m span was designed, and the finite element method was used to analyze its stiffness, strength, stability, and dynamic properties and the corresponding effects of the arch-axis coefficient, rise-span ratio, and span length on the mechanical properties. An experiment was carried out on a new deck arch bridge and a conventional deck arch bridge with the same span (10 m) to compare their performance. The results showed that the new deck arch bridge exhibited good mechanical properties while being inexpensive and easy to construct, which makes it suitable for high-speed railway bridges.
A Prospective Assessment of Knee Arthroscopy Skills Between Medical Students and Residents—Simulator Exercises for Partial Meniscectomy and Analysis of Learning Curves
Authors: Putzer David , Dammerer Dietmar , Baldauf Martina , Lenze Florian , Liebensteiner Michael C. , Nogler Michael 
Published: September 2021

Background The Covid-19 pandemic has created the largest disruption of education in history. In a response to this, we aimed to evaluate the knee arthroscopy learning curve among medical students and orthopaedic residents. Methods An arthroscopy simulator was used to compare the learning curves of two groups. Medical students with any prior knowledge of arthroscopy ( n=24) were compared to a residents group ( n=16). Analyzed parameters were “time to complete a task,” assessment of the movement of tools and values scoring damage to the surrounding tissues. Results After several repetitions, both groups improved their skills in terms of time and movement. Residents were on average faster, had less camera movement, and touched the cartilage tissue less often than did students. Students showed a steeper improvement curve than residents for certain parameters, as they started from a different experience level. Conclusion The participants were able to reduce the time to complete a task. There was also a decrease in possible damage to the virtual surrounding tissues. In general, the residents had better mean values, but the students had the steeper learning curve. Particularly less experienced surgeons can especially train their hand–eye coordination skills required for arthroscopy surgery. Training simulators are an important training tool that supplements cadaveric training and participation in arthroscopic operations and should be included in training.
New Chest Drainage Unit with Integrated CO2-Detector Demonstrates False Air Leak
Authors: Eckardt Jens , Lijkendijk Marike , Licht Peter B. , Stenger Michael 
Published: September 2021

Background Postoperative observed air leakage does not always originate from parenchymal defects but may arise from defects in the chest drainage unit, connections or reverse airflow in water seals. We investigated such false air leakage using a new chest drainage unit with a built-in CO2-detector and an electronic chest drainage unit. Methods Two types of chest drainage units were tested in a simple porcine model: A well-known electronic chest drainage unit and a new chest drainage unit with integrated CO2-detector. We created a setup of true air leakage—a parenchymal lesion, and false air leakage—allowing air to flow into the thoracic cavity alongside the chest drain. Results We demonstrated that the new chest drainage unit with a built-in CO2-detector can distinguish between experimentally induced true air leakage and false air leakage. Conclusion Available chest drainage systems do not allow direct assessment of true or false air leakage, which may increase chest drain duration unnecessarily. The integration of a CO2-sensitive color indicator into a chest drainage unit allows simple distinction between false air leak and true air leak, which may improve postoperative management.
A prototype of textile wastewater treatment using coagulation and adsorption by Fe/Cu nanoparticles: Techno-economic and scaling-up studies
Authors: Mahmoud Ahmed S , Mostafa Mohamed K , Peters Robert W 
Published: September 2021

This study aims to investigate the efficiency of a pilot prototype system comprising coagulation/flocculation, filtration, and nano-bimetallic iron/copper (Fe/Cu) degradation and adsorption units for the removal of chemical oxygen demand (COD), biological oxygen demand (BOD), color, total nitrogen (TN), total phosphorus (TP), and TSS from real textile wastewater. The total removal efficiencies of the system were 96, 98, 82, 69, 88, and 97%, respectively, using 0.5 g/L ferric chlorides as a coagulant under an optimum adsorption condition of pH 6.0, nano-dosage 1.4 g/L, contact time 80 min, and stirring rate 250 r/min at room temperature. Adsorption isotherms indicated that the removal of COD and TP obeys both Koble–Corrigan and Freundlich adsorption models, removal of color obeys both Koble–Corrigan and Hill adsorption models, and removal of TN and TSS obeys Koble–Corrigan and Khan models, respectively. Avrami kinetic models adequately describe the adsorption data for COD, BOD, TN, and TSS, while pseudo-second-order and intraparticle models described the removal mechanism of color and TSS, respectively. An artificial neural network (ANN) with r2-value exceeding 0.98 is accurate and can be used with confidence in predicting removal efficiencies of the targeted parameters. Sensitivity analysis results showed that the initial concentration was the most influential parameter for TSS removal with relative importance greater than 25%, while the bimetallic Fe/Cu dosage was the most influential factor for all other studied parameters with relative importance greater than 40%. The total treatment cost of the proposed system per m3 after scaling up was found to be US$4.5 for reuse of the treated water for the irrigation of forest trees.
Reconstruction of cardiovascular black-blood T2-weighted image by deep learning algorithm: A comparison with intensity filter
Authors: Ogawa Ryo , Kido Tomoyuki , Nakamura Masashi , Nozaki Atsushi , Lebel R Marc , Mochizuki Teruhito , Kido Teruhito 
Published: September 2021

Background Deep learning–based methods have been used to denoise magnetic resonance imaging. Purpose The purpose of this study was to evaluate a deep learning reconstruction (DL Recon) in cardiovascular black-blood T2-weighted images and compare with intensity filtered images. Material and Methods Forty-five DL Recon images were compared with intensity filtered and the original images. For quantitative image analysis, the signal to noise ratio (SNR) of the septum, contrast ratio (CR) of the septum to lumen, and sharpness of the endocardial border were calculated in each image. For qualitative image quality assessment, a 4-point subjective scale was assigned to each image (1 = poor, 2 = fair, 3 = good, 4 = excellent). Results The SNR and CR were significantly higher in the DL Recon images than in the intensity filtered and the original images ( p < .05 in each). Sharpness of the endocardial border was significantly higher in the DL Recon and intensity filtered images than in the original images ( p < .05 in each). The image quality of the DL Recon images was significantly better than that of intensity filtered and original images ( p < .001 in each). Conclusions DL Recon reduced image noise while improving image contrast and sharpness in the cardiovascular black-blood T2-weight sequence.
Clinical outcome and risk factors of patients with suspected pneumonia in Southwest district of Ethiopia, 2020: A retrospective study
Authors: Sebsibe Mengistu A , Kebede Molla A , Kazintet Yosef H , Gosaye Bizuayehu T , Teferi Addisalem M , Toru Milkiyas 
Published: September 2021

Acute respiratory infections (ARIs), especially pneumonia, remain the leading cause of childhood mortality and the most common reason for adult hospitalization in low- and middle-income countries, despite advances in preventative and management strategies. This study was conducted to assess factors associated with poor clinical outcome of suspected pneumonia cases among hospitalized patients at the three public health hospitals in Southwest district of Ethiopia. A cross-sectional study was conducted from May to July, 2020. Those patients admitted with suspected pneumonia were followed up during their hospital stay, and data on outcomes were captured by study nurses. Socio-demographics, clinical features, and follow-up data were gathered, and analyzed using SPSS versions 20.0. The differences in patients’ outcome in relation to their clinical features and epidemiologically linked exposures were described and compared using chi-square tests at 95% confident intervals. In this study, 742 patients with suspected pneumonia were analyzed. Of these, 473 (62.8%) of them were male, and 264 (35.6%) were of age 1–4 years. About 533 (71.8%) patients with suspected pneumonia were presented with irregular respiratory signs/symptoms (more than one symptoms) and 132 (17.9%) had underline illnesses. About 633 (85.4%) of patients were improved after treatment, and 109 (14.7%) of them ended with poor clinical outcome after completion of their treatment. Age of the patients and presence of comorbid conditions such as HIV/AIDS infections and bronchial asthma were identified as the risk factors for poor outcome of patients with suspected pneumonia. The clinical outcome of patients with suspected pneumonia at the three hospitals in Southwest district of Ethiopia was not satisfactory. Our findings highlight that in order to reduce poor clinical outcome related to suspected pneumonia, the efforts should be focused on some factors like management and prevention of chronic comorbidities. Increasing clinicians’ awareness on early management of suspected pneumonia cases is also essential in reducing the burden of the disease .
Developing a Simpler Prognosticating Tool: Comparing the Combined Assessment of Risk Encountered in Surgery Score with Deyo-Charlson Comorbidity Index and The American Society of Anesthesiologists Physical Status Score in Predicting 2 years Mortality after Hip Fracture Surgery
Authors: Cher Eric Wei Liang , Carson John Allen , Sim Eileen Yilin , Abdullah Hairil Rizal , Howe Tet Sen , Koh Suang Bee Joyce, 
Published: September 2021

Background: The use of risk stratification tools in identifying high-risk hip fracture patients plays an important role during treatment. The aim of this study was to compare our locally derived Combined Assessment of Risk Encountered in Surgery (CARES) score with the the American Society of Anesthesiologists physical status (ASA-PS) score and the Deyo–Charlson Comorbidity Index (D-CCI) in predicting 2-year mortality after hip fracture surgery. Methods and Material: A retrospective study was conducted on surgically treated hip fracture patients in a large tertiary hospital from Jan 2013 through Dec 2015. Age, gender, time to surgery, ASA-PS score, D-CCI, and CARES score were obtained. Univariate and multivariable logistic regression analyses were used to assess statistical significance of scores and risk factors, and area under the receiver operating characteristic (ROC) curve (AUC) was used to compare ASA-PS, D-CCI, and CARES as predictors of mortality at 2 years. Results: 763 surgically treated hip fracture patients were included in this study. The 2-year mortality rate was 13.1% (n = 100), and the mean ± SD CARES score of surviving and demised patients was 21.2 ± 5.98 and 25.9 ± 5.59, respectively. Using AUC, CARES was shown to be a better predictor of 2-year mortality than ASA-PS, but we found no statistical difference between CARES and D-CCI. A CARES score of 23, attributable primarily to pre-surgical morbidities and poor health of the patient, was identified as the statistical threshold for “high” risk of 2-year mortality. Conclusion: The CARES score is a viable risk predictor for 2-year mortality following hip fracture surgery and is comparable to the D-CCI in predictive capability. Our results support the use of a simpler yet clinically relevant CARES in prognosticating mortality following hip fracture surgery, particularly when information on the pre-existing comorbidities of the patient is not immediately available.
MutiLoc Nail Versus Philos Plate in Treating Proximal Humeral Fractures: A Retrospective Study Among the Alderly
Authors: Bu Guoyun , Sun Weitang , Li Jian’an , Yang Tao , Li Mingxin , Wei Wanfu 
Published: September 2021

Background Proximal humeral fractures (PHFs) account for 4–5% of all fractures in the elderly. There is still a controversy among the treatments in the displaced PHFs. Our aim was to explore the clinical outcome of PHFs with the treatment of MultiLoc nail or Philos plate in the elderly patients. Methods A total of 82 sustained elderly patients with PHFs were finally recruited between Dec 2016 and Dec 2017. 34 patients were treated with MultiLoc nail and 48 patients were treated with Philos plate. The demographics, fracture types, blood loss, operation time, union time, postoperative complications, visual analog scores (VASs), Constant scores, American Shoulder and Elbow Scores (ASESs), and neck-shaft-angle (NSA) between the two groups were compared. Results No differences were observed in the demographics, fracture types, VAS, Constant scores, and ASES scores between the two groups at final follow-up. Compared with the plate group, the blood loss, operation time, and union time were significantly lower in the nail group (all P < .05). The rate of general complications was 54.17% in the plate group, which was higher than that in the nail group (26.47%, P = .01). Three patients experienced reoperation in the plate group (3/48; 6.25%), but none in the nail group. Although there were no significant differences in intraoperative NSA between the two groups, the NSA at final follow-up in the nail group was much higher than the plate group (137.55 ± 5.53°vs 134.47 ± 5.92°, P = .02). Conclusions Multiloc intramedullary nail showed the similar effectiveness of final VAS, final Constant scores, and ASES scores in PHFs treatment with Philos plate. However, MultiLoc nail is superior to Philos plate in blood loss, operation time, complications, reoperation rate, and the change of NSA.