bluuebunny/crossref_metadata_2025_split huggingface dataset:
Disentangling the syntrophic electron transfer mechanisms of Candidatus geobacter eutrophica through electrochemical stimulation and machine learning
Authors: Yuan Heyang , Wang Xuehao , Lin Tzu-Yu , Kim Jinha , Liu Wen-Tso 
Published: July 2021

AbstractInterspecies hydrogen transfer (IHT) and direct interspecies electron transfer (DIET) are two syntrophy models for methanogenesis. Their relative importance in methanogenic environments is still unclear. Our recent discovery of a novel species Candidatus Geobacter eutrophica with the genetic potential of IHT and DIET may serve as a model species to address this knowledge gap. To experimentally demonstrate its DIET ability, we performed electrochemical enrichment of Ca. G. eutrophica-dominating communities under 0 and 0.4 V vs. Ag/AgCl based on the presumption that DIET and extracellular electron transfer (EET) share similar metabolic pathways. After three batches of enrichment, Geobacter OTU650, which was phylogenetically close to Ca. G. eutrophica, was outcompeted in the control but remained abundant and active under electrochemical stimulation, indicating Ca. G. eutrophica’s EET ability. The high-quality draft genome further showed high phylogenomic similarity with Ca. G. eutrophica, and the genes encoding outer membrane cytochromes and enzymes for hydrogen metabolism were actively expressed. A Bayesian network was trained with the genes encoding enzymes for alcohol metabolism, hydrogen metabolism, EET, and methanogenesis from dominant fermentative bacteria, Geobacter, and Methanobacterium. Methane production could not be accurately predicted when the genes for IHT were in silico knocked out, inferring its more important role in methanogenesis. The genomics-enabled machine learning modeling approach can provide predictive insights into the importance of IHT and DIET.
Superficial peroneal nerve entrapment in ankle sprain in childhood and adolescence
Authors: Falciglia Francesco , Basiglini Luca , Aulisa Angelo G. , Toniolo Renato M. 
Published: July 2021

AbstractTraumatic injuries of the ankle are the most common injuries in sports. Up to 40% of patients who have undergone inversion ankle sprain report residual symptoms. The primary purpose of the study is to evaluate the incidence of SPN entrapment as consequence of acute severe inversion ankle sprain in children and adolescents; the secondary is to report the diagnostic pathway and the results after surgical treatment. From 2000 to 2015 were reviewed to summarize patients under the age of 15 years treated for a first episode of severe inversion ankle sprain. Cases with persistent symptoms (more than 3 months) indicative for SPN neuropathy were then identified. Instrumental investigations were recovered and a pre-operative assessment of pain (VAS) was recorded. Patients were evaluated at minimum of 1-year post-operative follow-up. 981 acute ankle sprains have been evaluated. 122 were considered severe according to van Dijk criteria. 5 patients were considered affected by neuropathy of the SPN. All patients underwent surgery consisting in neurolysis and capsular retention and ligament reconstruction. At 25 months of follow-up AOFAS moved from 57.6 to 98.6. The study highlights a previously unreported condition of perineural fibrosis of the superficial peroneal nerve at the level of the ankle following first acute severe inversion ankle sprain in children.
Oxidative stress parameters as biomarkers of bladder cancer development and progression
Authors: Wigner Paulina , Szymańska Beata , Bijak Michał , Sawicka Ewa , Kowal Paweł , Marchewka Zofia , Saluk-Bijak Joanna 
Published: July 2021

AbstractThe epidemiological studies confirm that the overproduction of free radical is an important factor of cancer induction as well as development, and loss of antioxidant systems efficiency is associated with an increased risk of carcinogenesis. While bladder cancer is the fourth most common type of cancer all over the world, there is little evidence of the advancing changes in oxidative/nitrative stress during the progression of bladder cancer. Our study aimed to investigate the plasma levels of typical markers of oxidative/nitrative stress depending on the clinical classification of bladder cancer differentiation and infiltration degree. We examined 40 patients with newly diagnosed bladder cancer and 20 healthy volunteers as a control group. We analysed the plasma levels of protein carbonyls, thiol groups, 3-nitrotyrosine, lipid peroxidation, as well as non-enzymatic plasma antioxidant capacity using DPPH·and ABTS·+radicals. We confirmed that all analysed biomarkers are higher in enrolled BC patients than in healthy subjects. Furthermore, our findings demonstrate a positive correlation between the degree of bladder cancer progression and the level of oxidative stress, but no correlation in the case of NT-3. Based on obtained results, we might conclude that during carcinogenesis of the bladder increased oxidative damage of biomolecules is manifested. This indicates the participation of oxidative stress in the development of bladder cancer, and it is important the ensure the proper antioxidant protection.
Computed tomography angiography scoring systems and the role of skull defects in the confirmation of brain death
Authors: Zampakis Petros , Panagiotopoulos Vasilios , Kalogeropoulou Christina , Karachaliou Maria , Aretha Diamanto , Sioulas Nektarios , Dimoulia Sofia , Karnabatidis Dimitrios , Fligou Fotini 
Published: July 2021

AbstractTo assess and compare all current computed tomography angiography (CTA) scoring systems for the diagnostic workup of brain death (BD) to digital subtraction angiography (DSA) and clinical tests. Fifty-two patients with a clinical suspicion of BD underwent CTA and subsequently DSA. The diagnostic performance of all current CTA scoring systems was compared to that of DSA, in all patients with a suspicion of BD. A comparison to clinical tests was made only in DSA-positive for BD patients (n = 49), since in DSA-negative BD patients (n = 3) clinical tests were not performed. Further subgroup analysis was performed in relation to skull defects (SDs) stratification. Statistical analysis was conducted by applying statistics-contingency tables, Cochran’s-Q test and McNemar’s test. The CTA -10, and -7- and all 4-point scoring systems, showed overall sensitivities of 81,6%, 87.8% and 95.9% respectively and 100% specificity, when compared to DSA. In patients with a clinical verification of BD, the CTA -10 and -7-point scoring systems were significantly inferior to clinical tests (p = 0.004 and p = 0.031), while the 4-point scoring systems showed no such difference (p = 0.5). All 4-point scoring systems showed 100% sensitivity in patients with a minor SD or no SD. In patients with a major SD, all CTA scoring systems (− 10, − 7- and all 4-point) were less sensitive (62.5%, 62.5% and 75% respectively). The presence of a major SD was associated with an 8 × relative risk for false negative results in all 4-point scoring systems. CTA showed excellent diagnostic performance in patients with a suspicion of BD. The 4-point CTA scoring systems are the most sensitive for the diagnosis of BD, although in patients with a major SD patient, the role of CTA is ambiguous.
The anti-cancer drug dabrafenib is not cardiotoxic and inhibits cardiac remodelling and fibrosis in a murine model of hypertension
Authors: Meijles Daniel N. , Cull Joshua J. , Cooper Susanna T.E. , Markou Thomais , Hardyman Michelle A. , Fuller Stephen J. , Alharbi Hajed O. , Haines Zoe H.R. , Alcantara-Alonso Viridiana , Glennon Peter E. , Sheppard Mary N. , Sugden Peter H. , Clerk Angela 
Published: July 2021

Abstract
Raf kinases signal via extracellular signal-regulated kinases 1/2 (ERK1/2) to drive cell division. Since activating mutations in BRAF (B-Raf proto-oncogene, serine/threonine kinase) are highly oncogenic, BRAF inhibitors including dabrafenib have been developed for cancer. Inhibitors of ERK1/2 signalling used for cancer are cardiotoxic in some patients, raising the question of whether dabrafenib is cardiotoxic. In the heart, ERK1/2 signalling promotes not only cardiomyocyte hypertrophy and is cardioprotective but also promotes fibrosis. Our hypothesis is that ERK1/2 signalling is not required in a non-stressed heart but is required for cardiac remodelling. Thus, dabrafenib may affect the heart in the context of, for example, hypertension. In experiments with cardiomyocytes, cardiac fibroblasts and perfused rat hearts, dabrafenib inhibited ERK1/2 signalling. We assessed the effects of dabrafenib (3 mg/kg/d) on male C57BL/6J mouse hearts in vivo. Dabrafenib alone had no overt effects on cardiac function/dimensions (assessed by echocardiography) or cardiac architecture. In mice treated with 0.8 mg/kg/d angiotensin II (AngII) to induce hypertension, dabrafenib inhibited ERK1/2 signalling and suppressed cardiac hypertrophy in both acute (up to 7 d) and chronic (28 d) settings, preserving ejection fraction. At the cellular level, dabrafenib inhibited AngII-induced cardiomyocyte hypertrophy, reduced expression of hypertrophic gene markers and almost completely eliminated the increase in cardiac fibrosis both in interstitial and perivascular regions. Dabrafenib is not overtly cardiotoxic. Moreover, it inhibits maladaptive hypertrophy resulting from AngII-induced hypertension. Thus, Raf is a potential therapeutic target for hypertensive heart disease and drugs such as dabrafenib, developed for cancer, may be used for this purpose.
Postnatal inflammation in ApoE−/− mice is associated with immune training and atherosclerosis
Authors: Noye Ellesandra C. , Bekkering Siroon , Limawan Albert P. , Nguyen Maria U. , Widiasmoko Lisa K. , Lu Hui , Pepe Salvatore , Cheung Michael M. , Menheniott Trevelyan R. , Wallace Megan J. , Moss Timothy J. , Burgner David P. , Short Kirsty R. 
Published: July 2021

Abstract
Background and aims: Preterm birth is associated with increased risk of cardiovascular disease (CVD). This may reflect a legacy of inflammatory exposures such as chorioamnionitis which complicate pregnancies delivering preterm, or recurrent early-life infections, which are common in preterm infants. We previously reported that experimental chorioamnionitis followed by postnatal inflammation has additive and deleterious effects on atherosclerosis in ApoE−/− mice. Here, we aimed to investigate whether innate immune training is a contributory inflammatory mechanism in this murine model of atherosclerosis.
Methods: Bone marrow-derived macrophages and peritoneal macrophages were isolated from 13-week-old ApoE−/− mice, previously exposed to prenatal intra-amniotic (experimental choriomanionitis) and/or repeated postnatal (peritoneal) lipopolysaccharide (LPS). Innate immune responses were assessed by cytokine responses following ex vivo stimulation with toll-like receptor (TLR) agonists (LPS, Pam3Cys) and RPMI for 24-h. Bone marrow progenitor populations were studied using flow cytometric analysis.
Results: Following postnatal LPS exposure, bone marrow-derived macrophages and peritoneal macrophages produced more pro-inflammatory cytokines following TLR stimulation than those from saline-treated controls, characteristic of a trained phenotype. Cytokine production ex vivo correlated with atherosclerosis severity in vivo. Prenatal LPS did not affect cytokine production capacity. Combined prenatal and postnatal LPS exposure was associated with a reduction in populations of myeloid progenitor cells in the bone marrow.
Conclusions: Postnatal inflammation results in a trained phenotype in atherosclerosis-prone mice that is not enhanced by prenatal inflammation. If analogous mechanisms occur in humans, then there may be novel early life opportunities to reduce CVD risk in infants with early life infections.
Online Consumer Reviews on Hearing Health Care Services: A Textual Analysis Approach to Examine Psychologically Meaningful Language Dimensions
Authors: Manchaiah Vinaya , Swanepoel De Wet , Bennett Rebecca J. 
Published: July 2021

Purpose
The study examined psychologically meaningful language dimensions (i.e., social and emotional dimensions, health dimensions, and personal concerns) within online consumer reviews on hearing health care services using an automated textual analysis approach.

Method

Nine thousand six hundred twenty-two consumer reviews of hearing health care services, including an open-ended question (i.e., free text response to the prompt “share details of your own experience at this place”) and an overall rating (on a 5-point scale ranging from “very good” to “very poor”) were extracted from
Google.com
from 40 different cities across the United States. In addition, some metadata about the cities (i.e., region, population size, median age, percentage of older adults) were also recorded. Text responses were analyzed using the automated Linguistic Inquiry and Word Count software for selected language dimensions. The language dimensions of online hearing health care reviews were descriptively compared with language dimensions observed in the natural language of typical bloggers. Language dimensions from free text responses were correlated with quantitative overall experience ratings.

Results
Automated linguistic analysis showed that consumer reviews on hearing health care services had higher social processes, positive emotions, hearing, health, money, and work, and lower negative emotions and time-awareness when compared to typical bloggers. Examining the association between overall experience rating and the language dimensions revealed two broad findings. First, higher engagement of consumers in terms of social processes, positive emotions, hearing, and work dimensions was related to higher experience ratings. Second, higher engagement of consumers in terms of negative emotions, time awareness, and money was related to lower experience ratings.

Conclusions
Online reviews contain information about various dimensions (i.e., social and emotional dimensions as well as personal concerns) that have bearing toward the way in that they rate their health care experiences. Automated linguistic analysis of consumer reviews appears helpful in identifying gaps in service delivery that may influence consumer experience.
Inferring magnetic helicity spectrum in spherical domains: Method and example applications
Authors: Prabhu A. P. , Singh N. K. , Käpylä M. J. , Lagg A. 
Published: July 2021

Context. Obtaining observational constraints on the role of turbulent effects for the solar dynamo is a difficult, yet crucial, task. Without such knowledge, the full picture of the operation mechanism of the solar dynamo cannot be formed.
Aims. The magnetic helicity spectrum provides important information about the α effect. Here we demonstrate a formalism in spherical geometry to infer magnetic helicity spectra directly from observations of the magnetic field, taking into account the sign change of magnetic helicity across the Sun’s equator.
Methods. Using an angular correlation function of the magnetic field, we develop a method to infer spectra for magnetic energy and helicity. The retrieval of the latter relies on a fundamental definition of helicity in terms of linkage of magnetic flux. We apply the two-scale approach, previously used in Cartesian geometry, to spherical geometry for systems where a sign reversal of helicity is expected across the equator on both small and large scales.
Results. We test the method by applying it to an analytical model of a fully helical field, and to magneto-hydrodynamic simulations of a turbulent dynamo. The helicity spectra computed from the vector potential available in the models are in excellent agreement with the spectra computed solely from the magnetic field using our method. In a next test, we use our method to obtain the helicity spectrum from a synoptic magnetic field map corresponding to a Carrington rotation. We observe clear signs of a bihelical spectrum of magnetic helicity, which is in complete accordance to the previously reported spectra in literature from the same map.
Conclusions. Our formalism makes it possible to infer magnetic helicity in spherical geometry, without the necessity of computing the magnetic vector potential. It has many applications in solar and stellar observations, but can also be used to analyse global magnetoconvection models of stars and to compare them with observations.
Heating of the solar chromosphere in a sunspot light bridge by electric currents
Authors: Louis Rohan E. , Prasad Avijeet , Beck Christian , Choudhary Debi P. , Yalim Mehmet S. 
Published: July 2021

Context. Resistive Ohmic dissipation has been suggested as a mechanism for heating the solar chromosphere, but few studies have established this association.
Aims. We aim to determine how Ohmic dissipation by electric currents can heat the solar chromosphere.
Methods. We combine high-resolution spectroscopic Ca II data from the Dunn Solar Telescope and vector magnetic field observations from the Helioseismic and Magnetic Imager (HMI) to investigate thermal enhancements in a sunspot light bridge. The photospheric magnetic field from HMI was extrapolated to the corona using a non-force-free field technique that provided the three-dimensional distribution of electric currents, while an inversion of the chromospheric Ca II line with a local thermodynamic equilibrium and a nonlocal thermodynamic equilibrium spectral archive delivered the temperature stratifications from the photosphere to the chromosphere.
Results. We find that the light bridge is a site of strong electric currents, of about 0.3 A m−2 at the bottom boundary, which extend to about 0.7 Mm while decreasing monotonically with height. These currents produce a chromospheric temperature excess of about 600−800 K relative to the umbra. Only the light bridge, where relatively weak and highly inclined magnetic fields emerge over a duration of 13 h, shows a spatial coincidence of thermal enhancements and electric currents. The temperature enhancements and the Cowling heating are primarily confined to a height range of 0.4−0.7 Mm above the light bridge. The corresponding increase in internal energy of 200 J m−3 can be supplied by the heating in about 10 min.
Conclusions. Our results provide direct evidence for currents heating the lower solar chromosphere through Ohmic dissipation.
An all-sky sample of intermediate- to high-mass OBA-type eclipsing binaries observed by TESS
Authors: IJspeert L. W. , Tkachenko A. , Johnston C. , Garcia S. , De Ridder J. , Van Reeth T. , Aerts C. 
Published: July 2021

Context.Intermediate- to high-mass stars are the least numerous types of stars, and they are less well understood than their more numerous low-mass counterparts in terms of their internal physical processes. Modelling the photometric variability of a large sample of main-sequence intermediate- to high-mass stars in eclipsing binary systems will help to improve the models for such stars.Aims.Our goal is to compose a homogeneously compiled sample of main-sequence intermediate- to high-mass OBA-type dwarfs in eclipsing binary systems from TESS photometry. We search for binaries with and without pulsations and determine their approximate ephemerides.Methods.Our selection starts from a catalogue of dwarfs with colours corresponding to those of OBA-type dwarfs in the TESS Input Catalog. We develop a new automated method aimed at detecting eclipsing binaries in the presence of a strong pulsational and/or rotational signal relative to the eclipse depths and apply it to publicly available 30-min cadence TESS light curves.Results.Using targets with TESS magnitudes below 15 and cuts in the 2MASS magnitude bands ofJ − H < 0.045 andJ − K < 0.06 as the most stringent criteria, we arrive at a total of 189 981 intermediate- to high-mass candidates, 91 193 of which have light curves from at least one of two data reduction pipelines. The eclipsing binary detection and subsequent manual check for false positives resulted in 3155 unique OBA-type eclipsing binary candidates.Conclusions.Our sample of eclipsing binary stars in the intermediate- to high-mass regime allows for future binary (and asteroseismic) modelling with the aim to better understand the internal physical processes in this hot part of the main sequence.
12C/13C ratio and CNO abundances in the classical very old metal-poor dwarf HD 140283
Authors: Spite M. , Spite F. , Barbuy B. 
Published: July 2021

Context. The isotope abundances provide powerful diagnostics of the chemical enrichment in our Galaxy. The star HD 140283 is one of the best-studied very metal-poor dwarf stars. It is very old, and the chemical abundance in this star is a good witness of the chemical composition of the matter in the early Galaxy.
Aims. The aim of this work is to measure the precise abundances of carbon, nitrogen, oxygen, and mainly the 12C/13C isotopic ratio in this very old metal-poor star in order to have a good reference for the computations of the chemical evolution of the Galaxy.
Methods. We used very high spectral resolution data, with extremely high signal-to-noise ratios obtained with the spectrographs ESPaDOnS at the CFHT, ESPRESSO at the VLT, and HARPS at the ESO 3.6 m telescope.
Results. For the first time, we were able to measure the 12C/13C ratio in a very old metal-poor dwarf that was born at the very beginning of the Galaxy: 12C/13C = 33−6+12. We also obtained a precise determination of the abundance of the CNO elements in this star. These abundances give information about the early composition of the cloud from which HD 140283 was formed. They suggest that the effect of super-asymptotic giant branch stars or fast-rotating massive stars was significant in the early Galaxy.
Changes in the Total Solar Irradiance and climatic effects
Authors: Schmutz Werner K. 
Published: July 2021

The correlation between the averaged reconstructed March temperature record for Kyoto, Japan, and the reconstructed Total Solar Irradiance (TSI) over 660 years from 1230 to 1890 gives evidence with 98% probability that the Little Ice Age with four cold periods is forced by variations of TSI. If the correlation is restricted to the period 1650–1890, with two cold periods in the 17th and 19th century and for which two independent reconstructed March temperature records are available, the probability of solar forcing increases to 99.99%. As solar irradiance variations have a global effect there has to be a global climatic solar forcing impact. However, by how much global temperature were lower during these minima and with what amplitude TSI was varying is not accurately known. The two quantities, global temperature and TSI, are linked by the energy equilibrium equation for the Earth system. The derivation of this equation with respect to a variation of the solar irradiance has two terms: A direct forcing term, which can be derived analytically and quantified accurately from the Stefan-Boltzmann law, and a second term, describing indirect influences on the surface temperature. If a small TSI variation should force a large temperature variation, then it has to be the second indirect term that strongly amplifies the effect of the direct forcing. The current knowledge is summarized by three statements:During the minima periods in the 13th, 15/16th, 17th, and 19th centuries the terrestrial climate was colder by 0.5–1.5 °C;Indirect Top-down and Bottom-up mechanisms do not amplify direct forcing by a large amount, i.e. indirect solar forcing is of the same magnitude (or smaller) as direct solar forcing;The radiative output of the Sun cannot be lower by more than 2 Wm−2 below the measured present-day TSI value during solar cycle minimum.These three statements contradict each other and it is concluded that at least one is not correct. Which one is a wrong statement is presently not known conclusively. It is argued that it is the third statement and it is speculated that over centennial time scales the Sun might vary its radiance significantly more than observed so far during the last 40 years of space TSI measurements. To produce Maunder minimum type cold climate excursions, a TSI decrease of the order of 10 Wm−2 is advocated.