bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cortical Spreading Depression Can Be Triggered by Sensory Stimulation in Primed Wild Type Mouse Brain: a Mechanistic Insight to Migraine Aura Generation
Authors: Hanalioglu Sahin , Taskiran-Sag Aslihan , Karatas Hulya , Donmez-Demir Buket , Yilmaz-Ozcan Sinem , Eren-Kocak Emine , Gursoy-Ozdemir Yasemin , Dalkara Turgay 
Published: October 2021

Abstract
Background: Unlike the spontaneously appearing aura in migraineurs, experimentally, cortical spreading depression (CSD), the neurophysiological correlate of aura is induced by non-physiological stimuli. Consequently, neural mechanisms involved in spontaneous CSD generation, which may provide insight how migraine starts in an otherwise healthy brain, remains largely unclear. We hypothesized that CSD can be physiologically induced by sensory stimulation in primed mouse brain. Methods: Cortex was made susceptible to CSD with partial inhibition of Na+/K+-ATPase by epidural application of a low dose of Na+/K+-ATPase blocker ouabain that does not induce repetitive CSDs or by knocking-down α2 subunit of Na+/K+-ATPase, which is crucial for K+ and glutamate re-uptake by astrocytes, with shRNA. Stimulation-induced CSDs and extracellular K+ changes were monitored in vivo electrophysiologically or with a K+-sensitive fluoroprobe (IPG-4). Results: After priming with ouabain, photic stimulation increased the CSD incidence compared with non-stimulated animals (44.0 vs. 4.9%, p<0.001). Whisker stimulation was less effective (14.9 vs. 2.4%, p=0.02). Knocking-down Na+/K+-ATPase (50% decrease in mRNA) lowered the CSD threshold in all mice tested but triggered stimulus-induced CSDs in 14.3% and 16.7% of mice with photic and whisker stimulation, respectively. Confirming Na+/K+-ATPase hypofunction, extracellular K+ significantly rose during stimulation after subthreshold ouabain or shRNA treatment unlike controls. In line with higher CSD susceptibility, K+ rise was more prominent after ouabain. To gain insight to preventive mechanisms reducing the incidence of stimulus-induced CSDs, we applied an A1-receptor (DPCPX) or GABA-A (bicuculine) antagonist over the occipital cortex, because adenosine formed during stimulation or inhibitory interneuron activity can reduce CSD susceptibility. DPCPX induced CSDs or CSD-like small-DC shifts during photic stimulation, whereas bicuculine was not effective. Conclusions: Our findings indicate that normal brain is well protected against CSD generation. For CSD to be ignited under physiological conditions, priming and predisposing factors are required as seen in migraine patients. Intense sensory stimulation has the potential to trigger a CSD when co-existing conditions can bring extracellular K+ and glutamate concentrations over threshold via reduced uptake of K+ and glutamate (e.g. inefficient fueling of α2-Na+/K+-ATPase due to reduced glycogen breakdown) or facilitated glutamate release (e.g. reduced presynaptic adenosinergic inhibition).
Butterfly Glioblastoma - the Impact of Tumor Volumes and Treatment Strategies on Clinical Outcome
Authors: Bjorland Line Sagerup , Kurz Kathinka Dæhli , Fluge Fluge Øystein , Gilje Bjørnar , Mahesparan Rupavathana , Sætran Hege , Ushakova Anastasia , Farbu Elisabeth 
Published: October 2021

Abstract
PurposeButterfly glioblastoma is a rare subgroup of glioblastoma with a bihemispheric tumor crossing the corpus callosum, and is associated with a dismal prognosis. Prognostic factors are previously sparsely described and optimal treatment approaches remain uncertain. We aimed to analyse prognostic factors in butterfly glioblastoma, and to evaluate treatment strategies and outcome in a real-world setting.MethodsWe conducted a retrospective population-based cohort study of patients diagnosed with butterfly glioblastoma in Western Norway between 01/01/2007 and 31/12/2014. Clinical data were extracted from electronic medical records. Molecular and MRI volumetric analyses were retrospectively performed. Survival analyses were performed using Kaplan-Meier method and Cox proportional hazards regression models.ResultsAmong 381 patients diagnosed with glioblastoma, 36 patients (9.4%) met the criteria for butterfly glioblastoma. Median overall survival was 5.9 months (95% CI 2.7-9.2) and three-year survival was 8.3%. Age was strongly associated with poorer outcome, with adjusted hazard ratio (HR) 1.06 (95% CI 1.03-1.10), p<0.001. Best supportive care was associated with poorer survival compared to multimodal treatment (adjusted HR 5.69 (95% CI 1.39-23.38), p=0.02).ConclusionOutcome from butterfly glioblastoma was dismal, with a median overall survival of less than six months. However, long-term survival was comparable to that observed in glioblastoma in general, and multimodal treatment was associated with longer survival. This suggests that patients with butterfly glioblastoma may benefit from a more aggressive treatment approach despite the overall poor prognosis.
Theoretical Predictions on the Effective Piezoelectric Coefficients of Wurtzite LaN compound from DFT and DFPT Calculations
Authors: Almaghbash Z.A.A.R. , Omar Arbouche , Cherifi A. , Djellouli B. 
Published: October 2021

Abstract
To study the structural, elastic, piezoelectric and dielectric properties of wurtzite (LaN), we have used the ab initio total-energy pseudo-potential plane-wave method (PP-PW) based on density functional theory (DFT) and density functional perturbation theory (DFPT), which performed with Generalized gradient approximation (GGA) as well as local density approximation (LDA). The calculations of piezoelectric constants by mean of linear-response calculations second derivative with respect to a strain and a modification of the electric field. The compound shows a large effective piezoelectric constant (d33) being in the order of 66.96, and 77.260 pc/N with (GGA), and (LDA) calculations, respectively. The dielectric properties and electromechanical coupling coefficients have been calculated. Finally, our piezoelectric findings indicate that the LaN compound has a high effective piezoelectric constant value (d33) which allows it to be in direct competitor of zinc oxide ZnO and aluminum nitride AIN. We hope that our findings provide guidelines for the experimental realization and further research of high-performance materials appropriate for applications in different fields of study, such as biomedical engineering.
Parthanatos Involves in the Damage of Hippocampal Neurons Following Epileptic Seizure: in Vivo and Vitro Study
Authors: Wang Xue , Zhang Wuqiong , Ge Pengfei , Yu Miaomiao , Meng Hongmei 
Published: October 2021

Abstract
Epileptic seizures, or status epilepticus can cause hippocampal neurons death, which have several detrimental effects including promoting the progression of epilepsy, the formation of hippocampal sclerosis and cognitive impairment. Exploring the underlying molecular mechanisms of seizure-induced neuronal death may ameliorate the serious impact of the brain caused by seizures and restrain the further progression of epilepsy. Parthanatos is a new form of programmed cell death characterized by hyper-activation of Poly (ADP-ribose) polymerase-1 (PARP-1), excessive synthesis of poly ADP-ribose (PAR) polymer, mitochondrial depolarization and translocation of apoptosis-inducing factor (AIF) to nucleus which was found in various neurodegeneration disorders but rarely reported in the field of epilepsy. This study aims to investigate whether parthanatos was involved in the mechanism of seizure-induced neuronal death in vivo and vitro study, and to provide new targets for neuroprotective treatment of epilepsy. In vivo study, our results showed that PARP-1 inhibitor PJ34 has protective effect on the damage of hippocampal neurons in rats after epileptic seizure and reduced the expression level of PARP-1 and the synthesis of PAR polymer as well as the translocation of apoptosis-inducing factor (AIF) from mitochondrial to the nucleus. In vitro study, we used glutamate-induced HT22 cell injury to establish cell damage model. Our results showed that glutamate induces HT22 cells death in a time dependent manner, accompanied with parthanatos-related biochemical events. Pretreatment with PJ34 or knockdown of PARP-1 with small interfering RNA effectively protected HT22 cells against the toxic effect of glutamate, and attenuated parthanatos-related biochemical events. Further, application of NAC was found to rescue HT22 cells death and reverse the above-mentioned parthanatos-related biochemical events via alleviating the overproduction of reactive oxygen species (ROS). In conclusion, our study confirmed that Parthanatos was involved in the damage of hippocampal neurons following epileptic seizure, and ROS may be the initial factor of Parthanatos.
Effect of Initial Temperature on Iron Nugget Formation in Fluxless Processing of Titanomagnetite Under Isothermal – Temperature Gradient Profiles
Authors: Zulhan Zulfiadi , Sutandar Cheryl Livia , Suryani Indah , Basuki Eddy Agus 
Published: October 2021

Abstract
Recently, the titanomagnetite by coal producing iron nugget under isothermal – temperature gradient profile is introduced. The reduction process is divided into three stages where in the first stage the initial temperature is kept constant for a certain of time and then the temperature is increased to 1380°C with a certain heating rate in the second stage and finally in the third stage the temperature is maintained at 1380°C for a certain time. The results of the experiments show that the initial temperature in the first stage affects the formation of iron nuggets. Initial temperatures of 700 to 1100°C produce many nuggets up to 3 mm in size, initial temperature of 1200°C produces one nugget with a size of about 4 mm and the initial temperatures of 1300 and 1380°C do not produce nuggets. Optimal iron recovery is achieved at an initial temperature of 1000°C. The slag contains titanium oxide, armalcolite and rest of metallic iron with a size of less than 0.5 mm. The nugget formation is believed due to the aggregation and agglomeration of iron particle during the increasing temperature from initial stage to 1380°C.
Identification of a lncRNA-related Competing Endogenous RNA Network in Recurrent Implantation Failure
Authors: Huang Kai , Shi Ying , Chen Gezi 
Published: October 2021

Abstract
Background: Impaired endometrial receptivity is supposed to be a major element leading to recurrent implantation failure (RIF). Numerous studies have identified that the lncRNAs-miRNAs-mRNAs regulation network functions in the generation of receptive uterus. Long non-coding RNAs could act as competing endogenous RNAs in the pathogenesis of RIF. However, our understanding of the underlying mechanism is still limited. Results: Based on the RNA-Seq results, 617 DEmRNAs, 69 DElncRNAs and 107 DEmiRNAs were identified in the RIF group compared with the control group. To investigate the role of lncRNAs in RIF, we constructed a lncRNA related ceRNA network. A total of 3 lncRNAs, 8 miRNAs and 69 genes were identified. Above all, our study obtained 120 lncRNAs-miRNAs-mRNAs relationships in the ceRNA network. Among three hub lncRNAs, PART1 and PWRN1 were upregulated whereas PGM5P3-AS1 was downregulated in RIF endometrium. Meanwhile, three down-regulated miRNAs (hsa-miR-1207-5p, hsa-miR-134-5p, hsa-miR-1225-5p) and five up-regulated miRNAs (hsa-miR-30c-5p, hsa-miR-30b-5p, hsa-miR-145-5p, hsa-miR-21-5p, hsa-miR-196b-5p) were shown. Conclusions: We constructed a lncRNA-related ceRNA network and identified three hub lncRNAs in recurrent implantation failure. The results may provide further understanding in the pathogenesis of RIF as well as potential diagnostic and therapeutic targets.
Numerical Simulation of Multi-Cluster Fracture Propagation In Horizontal Wells With Limited-Entry Designs
Authors: Guo Changyong , Li Minghui , Liu Tao , Ma Huibo , Wang Bin , Jia Haizheng , Liang Tianbo 
Published: October 2021

Abstract
The effective propagation of multi-cluster fractures in horizontal wells is the key to the development of unconventional reservoirs. Due to the influence of pressure drops at perforating holes and the stress shadow effect, it is difficult to predict the fracturing fluid distribution and fracture dimensions in a fracturing stage. In this paper, a two-dimensional fluid-solid coupling model for simultaneous propagation of multiple fractures is established, and fluid distributions and dimensions of multiple fractures are studied with respect to different perforation designs. The model combines the User Amplitude Curve Subroutine (UAMP) in ABAQUS and the cohesive zone model (CZM) to calculate the perforating friction, fluid distribution and fracture propagation behaviors. After the accuracy of this model is verified by the analytical solution, a group of simulation is conducted to compare fracture propagations when the conventional limited-entry method (CLE) and extreme limited-entry method (less than 5 perforations per cluster, XLE) are used. Simulation results show that the edge and sub-central fractures in CLE cases almost get all the fluid and effectively propagate; central fractures receive little fluid and hardly propagate. In XLE cases, the fluid distribution of each fracture is relatively uniform, but the fracture lengths within one fracturing stage is still uneven; however, only reducing numbers or radii of perforation holes cannot achieve the uniform fracture propagation, where diverters might be further needed. Findings of this study provide a reference for the perforation optimization of multi-cluster horizontal wells in the field.
Hub Targets Analysis of miRNA-mRNA-TF Network in Diabetic Cardiomyopathy
Authors: Ren Bincheng , He Kaini , Yuan Miao , Wang Yu , Tie Yuanyuan , Gao Dengfeng 
Published: October 2021

Abstract
Background: The pathogenic mechanism and development of the diabetic cardiomyopathy(DCM) has been generally explained, and it is clear that the microRNAs(miRNAs), mRNAs and transcription factors(TFs) participate in the process of the DCM disease. Yet, the hub targets of the disease progression are not clear.Methods: To figure out the problem, we downloaded data sets from the Gene Expression Omnibus(GEO) database (GSE44179 and GSE4745). The targeted mRNAs of miRNAs were downloaded from TargetScan, miRBD and microT-CDS database. Gene Ontology (GO) enrichment of miRNAs and mRNAs were analysed in DAVID.R studio software was used to visualize the results of screened targets and GO enrichment. Cytoscape software was used to visualize the miRNA-mRNA-TF interaction network and calculate the hub targets. Results: We filtered eight miRNAs, nine mRNAs and ten transcription factors(TFs) by bioinformatics analysis, and constructed a miRNA-mRNA-TF network. The top ten degrees of nodes in the network are rno-miR-7a, Hnf4a, rno-miR-17, rno-miR-21, rno-miR-122, rno-miR-200c, Med1, Mlxipl, SP1 and rno-miR-34a, which were closely related to the process of DCM. Conclusion: This study revealed that rno-miR-7a, Hnf4a, rno-miR-17and rno-miR-21 may play vital role in the progress of diabetic cardiomyopathy.
Corticosteroid Treatment in Severe Patients With SARS-CoV-2 and Chronic HBV Co-infection: a Retrospective Multicenter Study
Authors: Meng Mei , Chu Yufeng , Zhang Sheng , Sha Jing , Wang Peng , Cui Yunliang , Han Meihong , Dong Xuan , Sun Wenqing , Zhang Zhongfa , Deng Yunxin , Wang Tao , Annane Djillali , Jia Shouqiang , Chen Dechang 
Published: October 2021

Abstract
Background. The impact of corticosteroids on severe patients with coronavirus disease 2019 (COVID-19)/ chronic hepatitis B virus (HBV) co-infection is currently unknown. We aimed to investigate effect of corticosteroid on these subgroup patients.Methods. In this retrospective multicenter study including 5447 confirmed COVID-19 patients from Jan 1, 2020 to Apr 18, 2020, severe patients with COVID-19/HBV co-infection were identified. To minimize the bias of confounding variables on effect of corticosteroid treatment, inverse probability of treatment weighting (IPTW) based on propensity score was employed.Results. The prevalence of HBV co-infection in hospitalization COVID-19 patients was 4.1%. 105 severe patients with COVID-19/HBV co-infection were enrolled (median age 62 years, 57.1% male). Fifty-five patients received corticosteroid treatment and 50 patients did not. Corticosteroid treatment was associated with high D-dimer level, neutrophil count (all P<0.05). With IPTW analysis, corticosteroid treatment worsen acute liver injury (OR, 1.767, 95%CI, 1.018-3.065, P=0.043). Corticosteroids might delay SARS-CoV-2 viral RNA clearance (OR, 4.963, 95%CI, 2.717-9.065, P <0.001). The 28-day and in-hospital mortality were both significantly higher in corticosteroid treatment group than non-corticosteroid treatment group (OR, 8.738, 95%CI, 2.826-27.022, P <0.001; OR, 10.122, 95%CI, 3.291-31.129, P <0.001, respectively). In multivariable analysis, higher D-dimer level (>1µg/ml) (OR, 10.686, 95%CI, 2.421-47.159, P=0.002) and corticosteroid therapy (OR, 11.236, 95%CI, 1.273-99.154, P=0.029) were independently associated with 28-day mortality. Methylprednisolone dose per day and cumulative dose in non-survivors was significantly higher than in survivors.Conclusions. In severe patients with COVID-19/HBV co-infection, corticosteroid treatment may increase mortality. Therefore, corticosteroid therapy should be prescribed with caution in the subset of patients.
Influence of sex on diaphragm thickness using a method for determining intima media thickness in healthy young adults
Authors: Oguri Masayoshi , Okanishi Tohru , Ikeguchi Takuya , Ogo Kaoru , Kanai Sotaro , Maegaki Yoshihiro , Wada Shinichi , Himoto Takashi 
Published: October 2021

Abstract
Background: To clarify the differences in diaphragm thickness between male and female participants in healthy young adults using ultrasonography and to investigate the relationship between diaphragm thickness and respiratory pressure.Methods: Twenty-nine healthy individuals (16 females and 13 males) participated in the study. Diaphragm thickness was measured at total lung capacity (TLC) and at functional residual capacity (FRC) in each participant. We measured the diaphragm thickness using a method for mean intima media thickness. Moreover, change ratio of diaphragm thickness was calculated with the diaphragm thickness at TLC and FRC.Results: Mean diaphragm thicknesses at FRC in males were significantly narrower than those in females (p<0.001). The change ratio of diaphragm thickness was significantly augmented in males compared with that in females (p<0.001). There was a significant positive correlation between the change ratio of diaphragm thickness and respiratory muscle strength in males but not in females.Conclusions: The change ratio might be an alternative to evaluate respiratory muscle strength in young adult males. The diaphragm thickness values and the change ratio of diaphragm thickness in healthy young adults, evaluated by B-mode sonography, were varied based on sex.
Preparation And Characterization of a New Proton-Exchange Membrane On The Basis of The Modification of Sulfonated Polystyrene With MIL-53 (Al)-NH2 For Applying in The Direct-Methanol Fuel Cell
Authors: Salehi Masoumeh , Karimipour Gholamreza , Montazerozohoori Morteza , Ghaedi Mehrorang , Mandanipour Valiollah 
Published: October 2021

Abstract
On the basis of the sulfonated polystyrene, a new proton-exchange membrane was prepared in this study. In order to be applied in the direct-methanol fuel cells (DMFCs), the modification of sulfonated polystyrene was conducted by MIL-53(Al)-NH2. Membranes were fabricated using sulfonated polystyrene, polyethylene, and different amounts of MIL-53(Al)-NH2, then they were briefly illustrated by SPS-PE-MIL-53. The membranes were characterized using the FT-IR, SEM, and TGA. The proton conductance, water uptake, oxidative resistance, ionic conductance, and the permeability of methanol were measured to assess its performance in a DMFC with a cation-exchange membrane. The SPS-PE-MIL-53 (1:1:0.25 w:w:w) membrane showed relatively better performance than the other membranes. The selectivity factor for this membrane was 7.17 × 104 S.s.cm-3. To assess the performance, the fabricated membranes were used in a fuel cell. All membranes fabricated in the fuel cell performed well, but the SPS-PE-MIL-53 (1:1:0.25 w:w:w) membrane performed better than the other membranes. DMFC on the basis of SPS-PE-MIL-53 (1:1:0.25 w:w:w) membrane had a maximum peak power density of 17.04 mAcm-2 with a maximum current density of 115.38 mAcm-2. In general, the prepared membranes performed well than similar membranes.
Proton Pump Inhibitor Intake During Chemoradiotherapy for Rectal Cancer: A Retrospective Study
Authors: Bridoux Marie , Deley Marie-Cecile Le , Bertrand Nicolas , Simon Nicolas , Sylla Dienabou , Mirabel Xavier , Turpin Anthony 
Published: October 2021

Abstract
Purpose: Proton pump inhibitors (PPIs) are one of the most widely used drugs worldwide and are involved in several drug interactions. Recently, several studies have suggested that PPIs may interfere with the efficacy of capecitabine. This study primarily aimed to investigate the effects of PPI intake on the pathological response rate of patients with locally advanced rectal cancer treated with neoadjuvant chemoradiotherapy with capecitabine. Method: A retrospective study was conducted at a French Comprehensive Cancer Center. Patients with locally advanced rectal cancer treated with neoadjuvant radiochemotherapy followed by surgery were included in the study. Demographic parameters, treatment characteristics, survival data, and PPI intake data were collected. Survival data were estimated using the Kaplan-Meier method and compared using the log-rank test.Results: In total, 215 patients were included, of whom 135 (62.8%) were men. The PPI intake frequency was 16.1%. The rate of complete histological response was lower in patients on PPIs than in those not on PPIs (8.7% vs. 19%, p=0.36). PPI intake was not associated with a statistically significant decrease in recurrence-free survival (hazard ratio [HR]=1.26, 95% confidence interval [CI] 0.61–2.60, p=0.54) or overall survival (HR=0.95, 95% CI 0.33–2.76, p=0.93).Conclusion: There is a trend to a lower complete histological response with PPI co-medication in patients treated for locally advanced rectal cancer. However, the safety of PPIs could not be confirmed. Further ancillary studies of prospective clinical trials or studies using the Health Data Hub are necessary to explore the effects of PPIs on rectal cancer more accurately.