bluuebunny/crossref_metadata_2025_split huggingface dataset:
Morphological, behavioral and molecular studies on CRISPR/Cas9-mediated knockout of the NOMO1 gene in zebrafish
Authors: Li Fei , Li Tingting , Lin Jia , Jian Jing , Zhang Qi , Zhang Yinglan , Liu Xiuyun , Ji Yongxia , Wang Xu , Li Qiang 
Published: January 2021

Abstract
Background: Multiple clinical genome-wide analysis identified that chromosome 16p13.11 is a hotspot associated with neuropsychiatric disorders such as autism, schizophrenia and epilepsy. Nodal modulator 1 (NOMO1), located on human chromosome 16p13.11, was considered as a candidate gene with neuropsychiatric disorders. However, it is unknown whether the nomo1 deficiency causes neurological abnormalities, and the molecular mechanisms and pathogenesis of the NOMO1 gene remain unclear. To study the effects of nomo1 deficiency on brain development and neuropsychiatric system, a nomo1 knockout zebrafish model was established.Methods: We developed a viable vertebrate model of nomo1 loss-of-function using CRISPR/Cas9 technology and characterized nomo1 mutant zebrafish. Phenotypic and functional studies of developing nomo1 mutant zebrafish, including morphological measurements, behavioral assays, and functional mechanistic analyses, were performed.Results: Morphological differences in the phenotype of nomo1-/- zebrafish gradually became less noticeable during development, however, the enlarged interstitial spaces in midbrain and hindbrain were detected in nomo1 mutant zebrafish. Meanwhile, the nomo1 deficiency caused the change of expression levels in neurotransmitters of γ-aminobutyrate, glutamate and serotonin. Interestingly, the nomo1 loss-of-function zebrafish model exhibited social defects and repetitive behaviors in juvenile, which represented autism-like behaviors. The transcriptome analysis showed different gene expression patterns in mutant zebrafish at the genetic level. Further results revealed that the neuroactive drug PTZ recovered the decreased locomotor phenotype in larval mutant zebrafish.Conclusions: In this study, we established a nomo1 vertebrate animal model using CRISPR/Cas9 gene editing approach. The loss-of-function of nomo1 displayed autism-like behaviors and altered levels of the γ-aminobutyrate, glutamate and serotonin in zebrafish, which provide evidence that nomo1 as a candidate gene for autism. The versatility of zebrafish model is contributed to studying NOMO1-related disorders and conducting drug screening in future.Limitations: Further studies are needed to determine whether an intervention with a neuroactive drug in nomo1-/- zebrafish to alter the behavioral phenotype is applicable to the behavior of human patients.
PREVENÇÃO DO BULLYING NA INFÂNCIA:
Authors: Popenga Geraim Monteiro Michelle , Lima-Berton Tatiane Delurdes de , Asinelli-Luz Araci 
Published: January 2021

Pensando na trajetória que ainda deve ser construída para a proteção da infância, discute-se a criança e os fatores de prevenção do bullying escolar, uma vez que existe a necessidade de aprofundar os saberes relacionados à prevenção, violações, violências e convivência escolar e a ampliação da experiência enquanto educadores, na tentativa de organizar ações e estratégias preventivas para infância, salientando a participação da instituição escolar, de espaços não escolares e a participação comunitária, como fontes de contribuição e corresponsabilidade. Trata-se de um estudo bibliográfico e com alcance descritivo. Uma discussão teórica que envolve autores sobre a criança e a Sociologia da Infância, sobre o bullying escolar e a prevenção à violência. A importância de abordar essas áreas da educação ocorre em razão da construção de vínculos afetivos tanto no ambiente escolar quanto em outros espaços com atividades que visam fortalecimento de vínculos e a convivência, nos quais o professor/educador trabalha em conjunto com profissionais que atuam fora da escola, envolvendo nessa perspectiva a participação da família e da comunidade.
Peningkatan Unjuk Kerja Pada Link Microwave Menggunakan Teknik Diversity Maximal Ratio Combining
Authors: Mudhoep Debbi Irfan , Sari Hartati Rukmi , Divayana Yoga 
Published: January 2021

Pada system komunikasi menggunakan gelombang microwave ( link microwave ), adanya noise serta multipath fading dapat mengurangi dan menurunkan kinerja system komunikasi. Perambatan gelombang mikro dipengaruhi beberapa hal pada system penerimaan selain harus LOS ( Line Of Sight ) terdapat sinyal pantulan dari permukaan yang sangat menimbulkan interferensi ( sinyal multipath ). Dengan menggunakan teknik diversity maximal ratio combining pada system yang terkena noise dan fading digunakan melalui analisa BER ( Bit Error Rate ). Penggunaan simulasi penelitian untuk mengetahui perbaikan system di penerima dengan membandingkan sebelum dan sesudah penggunaan teknik maximal ratio combining
Clinical Outcome Controversy in Helicobacter pylori Infection
Authors: Miftahussurur Muhammad , Oktaricha Heasty , Sugihartono Titong 
Published: January 2021

H. pylori infection can manifest as intestinal and extraintestinal disease. In this review, we summarize several factors that cause differences in clinical manifestations of H. pylori infection. Host inflammatory response and bacterial virulence are key in determining the pattern of acid secretion and gastritis. The acid level factor that has a greater effect, not H. pylori infection itself. Gastritis predominant in the gastric in the initial phase will produce higher acid and trigger GERD. H. pylori is also the cause of gastric adenocarcinoma. Different vacA genotypes are related to the risk of clinical manifestations such as peptic ulcer or gastric cancer. However, there is variance prevalence in some countries which can be explained through a combination of several factors including age at infection, virulence factors of H. pylori, host genetic profile, and environmental factors. Besides, important differences were found at a locus in CagA H. pylori which resulted in differences in clinical outcomes in the form of gastritis or MALT lymphoma. H. pylori infection alone is not enough to trigger idiopathic thrombocytopenic purpura (ITP). Additional triggers are needed to obtain an anti-platelet autoimmune response at ITP associated with H. pylori. Therefore, the difference in clinical manifestations of H. pylori infection remains controversial. H. pylori is not only a single cause, but the host and environmental factors also contribute to producing different responses.
Zdaniem polonistów – reforma w szkole podstawowej w świetle badań własnych
Authors: Karkut Dorota 
Published: January 2021

University of Rzeszów. Empirical research was carried out at the beginning of the 2018/2019 school year, ie one year after the implementation of the assumptions of the primary school education reform. They covered about 50 Polish language teachers in primary schools in the Podkarpacie region. The basis for the consideration were interviews conducted with Polish language teachers during the subject-methodological practice in the Polish language and observations of lessons, as well as questionnaires. Teachers evaluating the reform in primary school had the opportunity to express their opinions on possible difficulties in implementing
Zdaniem polonistów – reforma w szkole podstawowej w świetle badań własnych [99] the content of the Core curriculum, e.g in the area of planning and organization of wo
L'analyse de livres gradués (Escalire) par entremise du système d’analyse de texte par ordinateur (SATO)
Authors: Godin Alexie , Minor-Corriveau Michèle , Bélanger Roxanne 
Published: January 2021

Une série de livres gradués a été créée pour permettre aux élèves d’accéder à des livres correspondant à leurs habiletés en lecture (Éditions Passe-Temps, 2014). Cette série, nommée Escalire, contient 156 livres au total, qui sont répartis en 15 niveaux, soit de A à O. Cette échelle correspond aux niveaux établis par Fountas et Pinnell (2012). Cette série de livres gradués consiste en un programme d’intervention en lecture ciblant les élèves de la première à la quatrième année du cycle primaire. De tels programmes d’intervention en lecture peuvent mener l’élève vers l’acquisition et l’amélioration de la lecture en fonction de ses habiletés et son niveau scolaire. L’objectif de cette étude était de déterminer s’il y a corrélation entre les niveaux de lecture attribués aux livres de la série Escalire et le niveau de complexité indiqué par le système d’analyse de texte par ordinateur (SATO), pour ces mêmes livres. Les résultats ont démontré une corrélation modérée (r = 0.668, p < 0,001) et un pourcentage d’accord de 35,26 %. Somme toute, 95,51% des livres de la série Escalire se sont trouvés soit au niveau correspondant à l'échelle SATO ou à un niveau près (soit un niveau supérieur ou inférieur à ce qui a été proposé par SATO).
Mots Clés : Escalire, livres gradués, échelle de lecture Fountas et Pinnell, niveau de complexité, SATO
A STUDY OF USING POP SONGS TO PROMOTE NEW VOCABULARY LEARNING FOR SECONDARY SCHOOL STUDENTS
Authors: Borisai Thippawan , Dennis Nutprapha K. 
Published: January 2021

The purpose of this study aimed to1) promote students’ ability in learning new vocabulary through pop songs and 2) to investigate students’ opinions toward using pop songs in learning new vocabulary of grade 10 students at YangchumnoiPittayakom school, in Yangchumnoi district of Sisaket province. The sample who participated in this study were 40 grade 10 students at YangchumnoiPittayakom school derived by purposive sampling technique. The instruments were pretest and posttest, lesson plans and a questionnaire which was used to find the student’s opinions towards using pop songs method. The data collection were analyzed with the mean, standard deviation and t-test. The research finding of the study showed that teaching through pop songs increased students’ vocabulary ability for foreign language learners with significant value at .01 level. The students had the positive opinions toward using pop songs methods. It also revealed that the pop song technique was an effective tool to promote students’ vocabulary ability, made students cheerfully participate in class and increased motivation in learning English.
Reliability and construct validity of knowledge, attitude and practice of medical doctors on smoking cessation guidelines
Authors: Md Aris Mohd Aznan , Nik Mohamed Mohamad Haniki , Mohd Rus Razman , Jamani Nurjasmine Aida , Shalihin Mohd Shaiful Ehsan 
Published: January 2021

Introduction: Prevalence of active smokers in Malaysia is increasing despite availability of stop smoking clinics. Thus, practice of the healthcare professionals in providing smoking cessation intervention needs to be assessed. This study aimed to develop and validate a questionnaire to assess the knowledge, attitude and practice of medical doctors on national smoking cessation guidelines and factors contributing to the score. Methods: The 26 items consists of mixture of true/false questions; strongly-agree/agree/don’tknow/disagree/strongly-disagree response; and always/frequent/seldom/never response were created based on domain of 5A’s (ask, assess, advice, assist, arrange) and 5R’s (relevant, risks, rewards, roadblocks, repetitions). The questionnaires were distributed to 141 medical doctors. Reliability was determined using Cronbach’s alpha for internal consistency while construct validity was assessed using factor analysis. Results: A high degree of internal consistency was observed for this 26 items (Cronbach’s alpha= 0.824). Subsequently, 2 items with poor inter-item correlations were removed. Factor analysis extracted 7 meaningful components from this remaining 24 items, in which 3 components with least items were deleted due to overlapping subscale with other components, leaving 4 meaningful components of (1st) practice ask, advice, assess and practice 5R’s, (2nd) practice of assist and arrange for those willing to quit, (3rd) knowledge and (4th) attitude. These final 17 items still demonstrate high internal consistency with Cronbach’s alpha of 0.832. Conclusions: This study indicates that this questionnaire is a reliable and valid tool to assess the knowledge, attitude and practice on stop smoking guidelines. Improvement can be made for attitude items in the future.
Psychological Well-Being among Nursing Students
Authors: Al-Ani Ali Sabri Radeef 
Published: January 2021

Introduction: Nursing students are subjected to a variety of stressors during their study. Severe and prolonged stressors may affect the psychological well-being in the form of depression, anxiety and stress which may affect students’ academic performance, physical health and quality of life. Therefore, this study aimed to determine the rate and severity of depression, anxiety and stress symptoms and also to assess the relationship between these symptoms with stressors faced by the students. Materials and method: A sample of 174 nursing students from International Islamic University Malaysia participated in this study. Depression Anxiety, Stress Scale (DASS-21) was used to assess the psychological well-being by determining the prevalence and severity of depression, anxiety and stress (DAS) symptoms. The sources of stressors were identified by giving the students a list of the most possible source of stressors which were chosen depending on previous studies, and then the severity of stressors and their relationship with these symptoms were assessed. Results: The overall prevalence of depression, anxiety and stress symptoms was 51.7%, 81.6% and 43.1% respectively but it was found that 13.2%, 44.3% and 10.3% of nursing students have clinically significant depression, anxiety and stress respectively. No significant differences between the gender and age of students in relation to DAS symptoms. Regarding the source of stressors, the top five stressors decided by the students were fear of failing, examination and grades, study pressure and obligations, fear of unemployment after graduation and academic overload. Conclusion: Depression, anxiety and stress symptoms are present among nursing students which require early intervention. Academic factors can be considered as sources of stressors that may precipitate emotional disturbances among the nursing students.
Mechanisms of Colorectal Cancer Cell Growth and Metastasis Inhibition By Carp-1 Functional Mimetic-4
Authors: Ashour Abdelkader E. , Almuslim Anwar A. , Kumar Ashok , Zoheir Khairy M. A. , Ahmed Rehan , Ahmad Sheikh F. , Attia Sabry M. , AlGhamdi Khalid M. , Abd-Allah Adel R. A. 
Published: January 2021

Introduction: Colorectal cancer (CRC) constitutes one of the most aggressive malignancies worldwide and in Malaysia. Due to high recurrence rate and toxic side effects associated with radiation and chemotherapies, new agents are urgently needed. CARP-1 is a peri-nuclear phospho-protein which plays a dynamic role in regulating cell growth and apoptosis. CARP-1 functional mimetics (CFMs) are a class of compounds that stimulate CARP-1. CFM-4, a lead compound, was shown to suppress growth and metastasis of various cancers, other than CRC. We hypothesized that CFM-4 inhibits proliferation and metastasis in CRC. Materials and method: CFM-4 anti-cancer effects of on CRC cells were investigated using MTT assay, Annexin V/Propidium iodide (PI) apoptosis assay, cell cycle analysis, quantitative real-time PCR (qRT-PCR) and Western blotting. Antimetastatic activities were assessed by migration, colony formation and invasion assays. Results: CFM-4 inhibited CRC cell proliferation and was much more potent than the classical anti-CRC 5-fluorouracil. These effects were shown to be mediated at least in part by stimulating apoptosis, as indicated in our Annexin V/PI assay results. Cell cycle analysis showed that CFM-4 induced G2/M phase arrest. Molecularly, qRT-PCR results revealed that CFM-4 promoted intrinsic apoptosis by upregulating expression of caspase-8 and -9 , p53, PUMA and Noxa, and stimulated extrinsic apoptosis by enhancing expression of death receptors (DR4 and DR5). CFM-4 upregulated NF- k B signaling inhibitor A20-binding inhibitor protein and the PI3K negative regulator PTEN. Western blot analysis results revealed that CFM-4 enhanced expression of CARP1, caspase-8 and executioner caspase-3. Metastatic properties of the CRC cells were reduced by CFM-4 through blocking their capabilities to form colonies, migrate and invade through the matrix-coated membranes. Conclusion: The potent antitumor and anti-metastatic properties of CFM-4 against CRC are due to collective pro-apoptotic, anti-proliferative and anti-metastatic activities. Together our data warrants further investigations of CFM-4 as potential anti-tumor agent for CRC malignancy and metastasis.
Prevalence and Predictors of Pediculosis Capitis among Female Primary School Children in Kuantan, Pahang
Authors: Yunos Nur Aqilah , Mohd Tohit Nor Faiza , Mohd Rus Razman 
Published: January 2021

Introduction: Pediculosis capitis has been a worldwide concern among the vulnerable population of primary school children due to the physical, economical and psychological consequences that it brings to both the children and their caretakers. It is observed that there were only limited studies conducted in Malaysia and no previous study was done in Kuantan despite the health concern that it bears. Since both local and worldwide studies show that Pediculosis capitis is more prevalent among female as compared to male school children, thus, this study aims to investigate the prevalence and predictors of Pediculosis capitis among female primary school children in Kuantan. Materials and method: An analytical crosssectional study using cluster random sampling was carried out in Kuantan, Pahang, Malaysia. A self-administered validated questionnaire was used to collect the data among female primary school children from standard one to six. Hair and scalp examination was performed to identify presence of Pediculosis capitis infestation. Multivariate logistic regression was used to control for potential confounding and determine the predictors Results: 10 schools participated in the study with total number of 636 female students as respondents. The overall prevalence of Pediculosis capitis among female primary school children was 17.6% (n=112). Multivariate logistic regression analysis showed that Indian (OR=5.634, 95% CI= 3.157-10.055), history of contact with infested person (OR=2.822, 95% CI=1.6193.691) and those who had number of family members more than four (OR=0.065, 95% CI=0.008-0.550) were predictors for Pediculosis capitis Conclusion: The prevalence of Pediculosis capitis among female primary school children in Kuantan, Pahang is endemic (17.6%) and predictors are Indian, history of contact with infested person and having family members of more than 4. Hence, there is a need for health intervention to reduce the burden of Pediculosis capitis infestation.
Predicting Workplace Fecal Occult Blood Test Screening Uptake using Generalized Estimating Equations: A Sensitivity Analyses
Authors: MA Edre , KS Hayati , MS Salmiah , SI Sharifah Norkhadijah , MN Azmi 
Published: January 2021

Introduction: Many studies have shown the effectiveness of educational modules on increasing colorectal cancer screening uptake at individual level but not adjusted for potential clustering effect such as workplace. Longitudinal studies on workplace colorectal cancer screening require a series of analysis under different conditions due to heterogeneity of workplace population. To achieve this, a sensitivity analysis based on Generalized Estimating Equations was conducted to determine the robustness of the predictive performance of health education module in increasing screening uptake. Materials and Method: A parallel, single blind, cluster randomized trial was conducted among 15 organizations in Kuantan, Pahang. Intervention group received a complex Health Education Module comprising of group education, practical session on fecal occult blood test usage and WhatsApp group follow-up, while control group received standard colorectal cancer screening brochure. Sensitivity analyses using intention to treat analysis with interaction term, compatibility term, behavioral intention term and key assumption term were performed. Data were imputed and analysed using generalized estimating equation with IBM SPSS version 23. Pooled adjusted odds ratio was calculated using random effect model with inverse variance weighting using RevMan version 3.5. Results: A total of 166 participants from 15 organizations were recruited in the study. Intervention and control group were comparable at baseline (P>0.05). Health Education Module given in intervention group significantly increased the uptake of FOBT by nearly 5 times compared to control group in sensitivity analyses (pooled adjusted OR=4.60, 95% CI=2.65-7.99, I2=47%, P<0.001). Conclusions: Health Education Module was robust in facilitating fecal occult blood test uptake in different predictive models. Health Education Module should be implemented as a workplace health promotion program to improve colorectal cancer screening for early detection and prompt treatment.
Transforaminal Endoscopic Discectomy: A Targeted Spinal Surgery
Authors: Japar Khan Ed Simor Khan Mor , Yi Kow Ren 
Published: January 2021

Introduction: Open discectomy has been the gold standard of care for cases of Prolapsed intervertebral Disc. Tissue dissection is unavoidable in order to access the pathologic disc material. From skin incision, subcutaneous fat tissue, paraspinal muscles, ligamentum flavum, epidural fat, dura and nerve roots has to be encounter before reaching the disc material. With the in out transforaminal endoscopic technique only skin and subcutaneous tissue need to be bridge before reaching the disc material. This technique is also known as a targeted surgical technique. Minimal tissue injury is caused via this technique. Case report: We present 3 case series of prolapsed intervertebral disc which was treated with transforaminal endoscopic technique. Case 1: 49 year old presented with one year history of low back pain and left lower limb sciatica. Straight leg raising sign was positive. MRI showed 2 level disc bulge at L4L5, L5S1 with annular tear at L5S1. Case 2: 26 year old lady presented with 6 months history of low back pain and left lower limb sciatica. Straight leg raising sign was positive. MRI showed extruded disc at L4L5. Case 3: 28 year old lady presented with 8 months history of low back pain and left lower limb sciatica. Straight leg raising sign was positive. MRI showed extruded disc at L5S1 with annular tear. Results: All three patients underwent transformational discectomy. Case 1 and 2 were done under monitored anaesthesia control (MAC) and case 3 was done under epidural. All three patients had satisfactoryÂ pain relief with complete resolve of straight leg raising (SLR) test. The 1st patient had longer recovery period possibly because of its chronicity of symptoms. Conclusion: Early recovery is expected hence early return to work can be accomplish. In the future this might be the gold standard of treating prolapsed intervertebral disc.
Upmany i Anrikleje, Czuje i Nieczuje, czyli o wpływie nazw własnych na interpretację wybranych tomów poezji Jana Kasprowicza
Authors: Kiszka-Pytel Beata 
Published: January 2021

Artykuł dotyczy nazw własnych w poezji Jana Kasprowicza, wyekscerpowanych z – wybranych tomów jego poezji – Krzaku dzikiej róży, Księgi ubogich, Hymnów i wydanego pośmiertnie zbioru Mój świat. W analizach pominięte zostały ludowe antroponimy i górskie toponimy. Szczególną uwagę poświęcono natomiast dotychczas marginalizowanym relacjom zachodzącym pomiędzy onimami odnoszącymi się do sfery religijnej i do nazw produktów przemysłowych, a także ich wpływowi na interpretację spuścizny Kasprowicza.