bluuebunny/crossref_metadata_2025_split huggingface dataset:
Spatial and temporal distribution of anchovy density in Inner Ambon Bay on dry season (December-February)
Authors: Latumeten J , Latumeten V D V 
Published: June 2021

Abstract
Inner Ambon Bay is a small pelagic fish fishing ground, especially anchovy. Anchovy plays important role in the skipjack fishery as live bait in Ambon city. Nevertheless, research for its resource management is rarely done. The aims of the research are to obtain information of density, vertical distribution and horizontal distribution of anchovy on Dry Season. Densities data of anchovy were collected using hydro-acoustic device at six parallel transect lines and one cross- parallel transect line. Geo-statistical analysis technique was used to describe horizontal distribution of anchovy meanwhile, to observe vertical distribution pattern, vertical anchovy data was plotted on the graph. Result shows that lower average density of anchovy was found in January while higher density was in February. On vertical distribution, anchovy was distributed from near surface to 35 m depth layer, but higher densities were found at 25 m depth layer in December and February respectively and in January at 30 m of depth layer. On horizontal distribution, lower density of anchovy occupies a large space, in contrast, higher density was in small fish schools occupy a small space and are scattered in given parts of Inner Ambon Bay but at differ locations between months.
Community structure of conches (Strombus spp) in seagrass bed of Haria, Central Maluku, Indonesia
Authors: Uneputty P A , Lopulalan M , Natan Y , Pattikawa J A , Tetelepta J M S , Ongkers O T S 
Published: June 2021

Abstract
The coastal community especially women and children in Maluku usually utilize marine organisms when low tide or locally known as bameti. The gear use in this activity is simple such as spear, knife, and digger, or even by using bare hand. One of the target marine organisms is the conch (Strombus spp) that lives in the coastal area on a seagrass bed. This research was conducted to study the community structure of Strombus spp in Haria coastal waters, Central Maluku, Indonesia in February 2020. The sample was collected by using transect linear quadrate with the size of quadrate is 1൱m2. As many as 297 individuals of Strombus spp were collected during the study which consists of five species namely Strombus labiatus, S. mutabilis, S. urceus, S. gibberulus, and S. luhuanus.
S. labiatus has the highest density and occurrence frequency while the lowest belongs to S. luhuanus. Based on ecological indexes, Strombus spp in the area have high diversity and high evenness with low dominance.
Fattening of mangrove crab (Scylla serrata) in wooden pen and plastic container
Authors: Lopulalan Y , Pattikawa J A , Ode I , Wawo M , Natan Y , Tetelepta J M S , Ongkers O T S 
Published: June 2021

AbstractResearch to study fattening of mangrove crab (Scylla serrata) was conducted in the coastal waters of Wael Village, Western Seram Regency, Maluku Province Eastern Indonesia from September to October 2018. Two methods were used for fattening namely wooden pen and plastic container. Totally, there are 54 individuals of mangrove crab used in this study which consists of 30 individuals maintained in wooden pen and 24 individual kept in plastic container during five periods of research for two months. The results showed that at the end of the research, mangrove crabs kept in wooden pen had better growth than those maintained in plastic container. Even though they had same survival, the results of economic analysis showed that only mangrove crab in the wooden pen have a good price so that the investment gets a profit, while investing in mangrove crabs kept in plastic container becomes a loss.
Business feasibility assessment of floating cage system of trevallies in Inner Ambon Bay
Authors: Ayal F W , Lewerissa Y A 
Published: June 2021

Abstract
Study on the feasibility of a floating cage cultivation business of Trevally in Inner Ambon Bay (IAB) waters was done to analyse the production factors of floating cage system and to analyse the feasibility business of floating cage system in IAB. This research was conducted in IAB considering most of floating cage distribution are located in the area. Data collection was carried out on floating cage cultivation business units in IAB waters, which consisted of 10 cultivation units according to the management group. Result of this research showed that the factors of production in the Trevallies aquaculture business unit in IAB are supply of feed that cover mostly operational cost and unavailability of feed suppliers, supply of fish seeds from Marine Cultivation and Seed Center Waiheru Ambon and the availability of labour ranging from 2-5 people per business unit. The cultivation of Trevallies in IAB using the floating cage method is very feasible and profitable to run.
The sensory profile of Lutjanus sp and Cephalopholis sp kept in Atung Parinarium glaberimum Hassk powder, ice and its combination
Authors: Moniharapon T , Pattipeilohy F , Moniharapon D L 
Published: June 2021

Abstract
Nowadays bioactive compound from plant are used to support pharmacological industry, while in food industry it is encouraged as preservatives, either as antioxidant or antibacterial. Atung plant (Parinarium glaberimum Hassk) is endemic in Mollucas. Fruits of Atung have been used for hundred of years by the local fishermen when they go fishing. Efforts to preserved fresh food especially fish were continually pursue by using natural preserver which contained bioactive compound as anti-bacteria both patogen and spoilage. The purpose of the study was to investigate the effectivenes and to get information on the sensory quality of post-harvested using Lutjanus sp. and Cephalopholis sp. kept in atung, ice and a combination of atung and ice. The fishes were kept in 0.3% atung (W/W). ice (1:1) and a mixture of atung and ice, with shelf-life of freshness of 0, 6, 12, 18, 24 and 30 hours. Sensory quality test was conducted by using descriptive test with scale ranged from 1-9. Quality parameters observed were the appearance consist of eyes, gill, body surface, slime, flesh and stomach; odour; and consistency. Firstly, the results showed that treatment atung and ice is the prime sensory quality was at 18th with scale values of 8.2 and 18th with scale values of 8.2 for Lutjanus sp. and Cephalopholis sp., respectively. Secondly, the results showed that sensory quality of postharvested fresh Lutjanus sp. kept in atung and ice was the best treatment and with longer shelf-life of freshness, i.e 36 hours at 6.1 scale. Application of atungs powder were more practical, efficient and cheaper than ice. As an alternative preservative fresh fish food by the fishermen. It is the best strategic in Mollucas because atung is endemic plant. It’s can be applied to archipelago community in Mollucas with the characteristic of small islands.
Small island women in the sustainability of household livelihoods: case study in Romang Island, Southwest Maluku Regency
Authors: Sopamena J F , Pattiselanno A E 
Published: June 2021

Abstract
Women play important role in fulfilling household needs. Apart from carrying out domestic activities, women are also active in public activities as an effort to maintain the sustainability of household livelihoods. This research is aimed to analyze the position of women in the sustainability of household livelihoods. Qualitative approach was used where data were collected with in-depth interview. Informants were selected using snowball method, and eight women were obtained. Result of research showed that women start to enter public world and seem capable to cope with public complexities. Public world is a place where work orientation is shifting from subsistence to commercial. Work commercialization requires women to do more varying activities including selling production outputs. Women must wander through villages or village markets in order to get a good sale. Although the activities are varying with livelihoods sources, but those represent the effort to maintain the sustainability of household livelihoods.
Recent progress of the Computational 2D Materials Database (C2DB)
Authors: Gjerding Morten Niklas , Taghizadeh Alireza , Rasmussen Asbjørn , Ali Sajid , Bertoldo Fabian , Deilmann Thorsten , Knøsgaard Nikolaj Rørbæk , Kruse Mads , Larsen Ask Hjorth , Manti Simone , Pedersen Thomas Garm , Petralanda Urko , Skovhus Thorbjørn , Svendsen Mark Kamper , Mortensen Jens Jørgen , Olsen Thomas , Thygesen Kristian Sommer 
Published: June 2021

Abstract
The Computational 2D Materials Database (C2DB) is a highly curated open database organising a wealth of computed properties for more than 4000 atomically thin two-dimensional (2D) materials. Here we report on new materials and properties that were added to the database since its first release in 2018. The set of new materials comprise several hundred monolayers exfoliated from experimentally known layered bulk materials, (homo)bilayers in various stacking configurations, native point defects in semiconducting monolayers, and chalcogen/halogen Janus monolayers. The new properties include exfoliation energies, Bader charges, spontaneous polarisations, Born charges, infrared polarisabilities, piezoelectric tensors, band topology invariants, exchange couplings, Raman spectra and second harmonic generation spectra. We also describe refinements of the employed material classification schemes, upgrades of the computational methodologies used for property evaluations, as well as significant enhancements of the data documentation and provenance. Finally, we explore the performance of Gaussian process-based regression for efficient prediction of mechanical and electronic materials properties. The combination of open access, detailed documentation, and extremely rich materials property data sets make the C2DB a unique resource that will advance the science of atomically thin materials.
Strong convexity for harmonic functions on compact symmetric spaces
Authors: Lippner Gabor , Mangoubi Dan , McGuirk Zachary , Yovel Rachel 
Published: June 2021

Let

h
h

be a harmonic function defined on a spherical disk. It is shown that

Δ
k

|

h

|

2

\Delta ^k |h|^2

is nonnegative for all

k
∈

N

k\in \mathbb {N}

where

Δ
\Delta

is the Laplace-Beltrami operator. This fact is generalized to harmonic functions defined on a disk in a normal homogeneous compact Riemannian manifold, and in particular in a symmetric space of the compact type. This complements a similar property for harmonic functions on

R

n

\mathbb {R}^n

discovered by the first two authors and is related to strong convexity of the

L
2

L^2

-growth function of harmonic functions.
Skin Tightening With Hyperdilute CaHA: Dilution Practices and Practical Guidance for Clinical Practice
Authors: Lorenc Z Paul , Black Jeanette M , Cheung Jessie S , Chiu Annie , Del Campo Roberta , Durkin Alan James , Graivier Miles , Green Jeremy B , Kwok Gideon P , Marcus Keith , Rammos Charalambos “Babis” , Werschler William Philip 
Published: June 2021

Abstract

Background
Over the past several years, hyperdilute calcium hydroxylapatite (CaHA) has emerged as an effective modality for improving skin quality and managing laxity in the face, arms, hands, neck, décolletage, upper arms, abdomen, buttocks, and upper legs, as well as for treating cellulite and striae. Whereas undiluted CaHA is used to provide volume, hyperdilute CaHA is distributed across a much larger surface area in a more superficial plane to stimulate neocollagenesis and elastin formation over time. The absence of lymphocytic infiltrates and predominance of type 1 collagen in the tissue response to CaHA make hyperdilute CaHA a valuable tool for nonsurgical skin tightening.

Objectives
The aim of this study was to provide practical step-by-step guidance on patient selection, dilution practices, and optimal injection technique to facilitate incorporation of the technique into clinical practice.

Methods
Over the course of 3 regional meetings in the United States, 12 expert physician injectors participated in live webinars as part of a continuing medical education program.

Results
The practical guidance in this manuscript is based upon the most frequently requested information by audience members and the information considered critical for success by the authors.

Conclusions
The minimally invasive nature of filler injection results in little downtime, making this treatment particularly appealing. The recommendations presented are consistent with previously published consensus guidelines on hyperdilute CaHA but are intended to serve as “how-to” guidance based on the experience of expert injectors who have successfully treated the face and body.

Level of Evidence: 4
MiR-335-3p inhibits cell proliferation and induces cell cycle arrest and apoptosis in acute myeloid leukemia by targeting EIF3E
Authors: Zhang Ling , Wang Xiaozhen , Wu Jieying , Xiao Ruozhi , Liu Jiajun 
Published: June 2021

ABSTRACT
Here, we aimed to investigate the biological roles and the regulatory mechanisms of miR-335-3p in acute myeloid leukemia (AML). We first found miR-335-3p was significantly downregulated in blood samples from leukemia patients and cell lines using reverse transcription quantitative polymerase chain reaction. Through CCK-8 assay and flow cytometry, we observed that miR-335-3p overexpression significantly inhibited cell proliferation and induced cell cycle G0/G1 arrest and apoptosis in AML cell lines (THP-1 and U937). Moreover, miR-335-3p directly targets EIF3E and negatively regulated its expression. More importantly, EIF3E overexpression reversed the effects of miR-335-3p on cell proliferation, G1/S transition, and apoptosis. Furthermore, miR-335-3p overexpression obviously downregulated the expression of CDK4, Cyclin D1, and Bcl-2, while upregulated the expression of p21 and Bad, which were significantly rescued by the cotransfection of pcDNA3.1-EIF3E. Collectively, our study proposes that miR-335-3p/EIF3E axis could be a promising therapeutic target to mitigate the progression of AML.
phyLoSTM: a novel deep learning model on disease prediction from longitudinal microbiome data
Authors: Sharma Divya , Xu Wei 
Published: June 2021

AbstractMotivationResearch shows that human microbiome is highly dynamic on longitudinal timescales, changing dynamically with diet, or due to medical interventions. In this article, we propose a novel deep learning framework ‘phyLoSTM’, using a combination of Convolutional Neural Networks and Long Short Term Memory Networks (LSTM) for feature extraction and analysis of temporal dependency in longitudinal microbiome sequencing data along with host’s environmental factors for disease prediction. Additional novelty in terms of handling variable timepoints in subjects through LSTMs, as well as, weight balancing between imbalanced cases and controls is proposed.ResultsWe simulated 100 datasets across multiple time points for model testing. To demonstrate the model’s effectiveness, we also implemented this novel method into two real longitudinal human microbiome studies: (i) DIABIMMUNE three country cohort with food allergy outcomes (Milk, Egg, Peanut and Overall) and (ii) DiGiulio study with preterm delivery as outcome. Extensive analysis and comparison of our approach yields encouraging performance with an AUC of 0.897 (increased by 5%) on simulated studies and AUCs of 0.762 (increased by 19%) and 0.713 (increased by 8%) on the two real longitudinal microbiome studies respectively, as compared to the next best performing method, Random Forest. The proposed methodology improves predictive accuracy on longitudinal human microbiome studies containing spatially correlated data, and evaluates the change of microbiome composition contributing to outcome prediction.Availability and implementationhttps://github.com/divya031090/phyLoSTM.Supplementary informationSupplementary data are available at Bioinformatics online.
Increasing the accuracy of single sequence prediction methods using a deep semi-supervised learning framework
Authors: Moffat Lewis , Jones David T 
Published: June 2021

Abstract

Motivation
Over the past 50 years, our ability to model protein sequences with evolutionary information has progressed in leaps and bounds. However, even with the latest deep learning methods, the modelling of a critically important class of proteins, single orphan sequences, remains unsolved.

Results
By taking a bioinformatics approach to semi-supervised machine learning, we develop Profile Augmentation of Single Sequences (PASS), a simple but powerful framework for building accurate single-sequence methods. To demonstrate the effectiveness of PASS we apply it to the mature field of secondary structure prediction. In doing so we develop S4PRED, the successor to the open-source PSIPRED-Single method, which achieves an unprecedented Q3 score of 75.3% on the standard CB513 test. PASS provides a blueprint for the development of a new generation of predictive methods, advancing our ability to model individual protein sequences.

Availability and implementation
The S4PRED model is available as open source software on the PSIPRED GitHub repository (https://github.com/psipred/s4pred), along with documentation. It will also be provided as a part of the PSIPRED web service (http://bioinf.cs.ucl.ac.uk/psipred/).

Supplementary information
Supplementary data are available at Bioinformatics online.
Activation of α7 nicotinic acetylcholine receptor ameliorates HIV-associated neurology and neuropathology
Authors: Zhao Xiaojie , Wilson Kelly , Uteshev Victor , He Johnny J 
Published: June 2021

AbstractHIV-associated neurocognitive disorders (HAND) in the era of combination antiretroviral therapy are primarily manifested as impaired behaviours, glial activation/neuroinflammation and compromised neuronal integrity, for which there are no effective treatments currently available.In the current study, we used doxycycline-inducible astrocyte-specific HIV Tat transgenic mice (iTat), a surrogate HAND model, and determined effects of PNU-125096, a positive allosteric modulator of α7 nicotinic acetylcholine receptor (α7 nAChR) on Tat-induced behavioural impairments and neuropathologies.We showed that PNU-125096 treatment significantly improved locomotor, learning and memory deficits of iTat mice while inhibited glial activation and increased PSD-95 expression in the cortex and hippocampus of iTat mice. Using α7 nAChR knockout mice, we showed that α7 nAChR knockout eliminated the protective effects of PNU-125096 on iTat mice. In addition, we showed that inhibition of p38 phosphorylation by SB239063, a p38 MAPK-specific inhibitor exacerbated Tat neurotoxicity in iTat mice. Last, we used primary mouse cortical individual cultures and neuron-astrocytes co-cultures and in vivo staining of iTat mouse brain tissues and showed that glial activation was directly involved in the interplay among Tat neurotoxicity, α7 nAChR activation and the p38 MAPK signalling pathway.Taken together, these findings demonstrated for the first time that α7 nAChR activation led to protection against HAND and suggested that α7 nAChR modulator PNU-125096 holds significant promise for development of therapeutics for HAND.
Improving Outpatient Antibiotic Prescribing for Respiratory Tract Infections in Primary Care: A Stepped-Wedge Cluster Randomized Trial
Authors: Dutcher Lauren , Degnan Kathleen , Adu-Gyamfi Afia B , Lautenbach Ebbing , Cressman Leigh , David Michael Z , Cluzet Valerie , Szymczak Julia E , Pegues David A , Bilker Warren , Tolomeo Pam , Hamilton Keith W 
Published: June 2021

Abstract

Background
Inappropriate antibiotic prescribing is common in primary care (PC), particularly for respiratory tract diagnoses (RTDs). However, the optimal approach for improving prescribing remains unknown.

Methods
We conducted a stepped-wedge study in PC practices within a health system to assess the impact of a provider-targeted intervention on antibiotic prescribing for RTDs. RTDs were grouped into tiers based on appropriateness of antibiotic prescribing: tier 1 (almost always indicated), tier 2 (may be indicated), and tier 3 (rarely indicated). Providers received education on appropriate RTD prescribing followed by monthly peer comparison feedback on antibiotic prescribing for (1) all tiers and (2) tier 3 RTDs. A χ 2 test was used to compare the proportion of visits with antibiotic prescriptions before and during the intervention. Mixed-effects multivariable logistic regression analysis was performed to assess the association between the intervention and antibiotic prescribing.

Results
Across 30 PC practices and 185 755 total visits, overall antibiotic prescribing was reduced with the intervention, from 35.2% to 23.0% of visits (P &lt; .001). In multivariable analysis, the intervention was associated with a reduced odds of antibiotic prescription for tiers 2 (odds ratio [OR] 0.57; 95% confidence interval [CI] .52–.62) and 3 (OR 0.57; 95% CI .53–.61) but not for tier 1 (OR 0.98; 95% CI .83–1.16).

Conclusions
A provider-focused intervention reduced overall antibiotic prescribing for RTDs without affecting prescribing for infections that likely require antibiotics. Future research should examine the sustainability of such interventions, potential unintended adverse effects on patient health or satisfaction, and provider perceptions and acceptability.
Prevalence of Oral Human Papillomavirus Infection: Impact of Sex, Race/Ethnicity, and Vaccination Status
Authors: Berenson Abbey B , Hirth Jacqueline M , Chang Mihyun 
Published: June 2021

Abstract

Background
Prior studies have demonstrated differences in oral human papillomavirus (HPV) prevalence by sex and race/ethnicity. In this study, we examined the impact of vaccination on these disparities.

Methods
We examined participants aged 18–59 years in the National Health and Nutrition Examination Survey from 2011 to 2016 who reported their HPV vaccination status and submitted an adequate oral sample (N = 9437). Oral prevalence of HPV, grouped by any, low-risk, high-risk, 4 valent (4v) HPV, 9 valent (9v) HPV, and nonvaccine types, was examined by sex, race/ethnicity, and vaccination status. Binary logistic regression was used to estimate prevalence ratios by vaccination status. Multivariable logistic regression models controlled for age, sex, and race/ethnicity.

Results
The prevalence of any, nonvaccine, low-risk, high-risk, 4vHPV, and 9vHPV types was higher among males than females, even among vaccinated participants. Examination of racial/ethnic differences demonstrated differences in all HPV groups among unvaccinated males and among low-risk types in females. In all but the 2 vaccine-type groups, the prevalence of oral HPV was notably higher among Black males compared with other groups. Significant differences were not observed by race/ethnicity among vaccinated males or females.

Conclusions
Males tested positive for oral HPV more frequently than females, even among those vaccinated. This may have resulted from a lower frequency of males being vaccinated before initiating oral sex than females. Vaccination of males at the recommended age, therefore, may decrease differences in oral HPV by sex. Racial/ethnic differences were observed only in unvaccinated individuals, suggesting these disparities will decrease as more individuals are vaccinated.