bluuebunny/crossref_metadata_2025_split huggingface dataset:
FBXO16-mediated hnRNPL ubiquitination and degradation plays a tumor suppressor role in ovarian cancer
Authors: Ji Mei , Zhao Zhao , Li Yue , Xu Penglin , Shi Jia , Li Zhe , Wang Kaige , Huang Xiaotian , Ji Jing , Liu Wei , Liu Bin 
Published: July 2021

AbstractHeterogeneous nuclear ribonucleoprotein L (hnRNPL) is a type of RNA binding protein that highly expressed in a variety of tumors and plays a vital role in tumor progression. However, its post-translational regulation through ubiquitin-mediated proteolysis and the cellular mechanism responsible for its proteasomal degradation remains unclear. F-box proteins (FBPs) function as the substrate recognition subunits of SCF ubiquitin ligase complexes and directly bind to substrates. The aberrant expression or mutation of FBPs will lead to the accumulation of its substrate proteins that often involved in tumorigenesis. Here we discover FBXO16, an E3 ubiquitin ligase, to be a tumor suppressor in ovarian cancer, and patients with the relatively high expression level of FBXO16 have a better prognosis. Silencing or depleting FBXO16 significantly enhanced ovarian cancer cell proliferation, clonogenic survival, and cell invasion by activating multiple oncogenic pathways. This function requires the F-box domain of FBXO16, through which FBXO16 assembles a canonical SCF ubiquitin ligase complex that constitutively targets hnRNPL for degradation. Depletion of hnRNPL is sufficient to inactive multiple oncogenic signaling regulated by FBXO16 and prevent the malignant behavior of ovarian cancer cells caused by FBXO16 deficiency. FBXO16 interacted with the RRM3 domain of hnRNPL via its C-terminal region to trigger the proteasomal degradation of hnRNPL. Failure to degrade hnRNPL promoted ovarian cancer cell proliferation in vitro and tumor growth vivo, phenocopying the deficiency of FBXO16 in ovarian cancer.
Fibrillar α-synuclein induces neurotoxic astrocyte activation via RIP kinase signaling and NF-κB
Authors: Chou Tsui-Wen , Chang Nydia P. , Krishnagiri Medha , Patel Aisha P. , Lindman Marissa , Angel Juan P. , Kung Po-Lun , Atkins Colm , Daniels Brian P. 
Published: July 2021

AbstractParkinson’s disease (PD) is a neurodegenerative disorder characterized by the death of midbrain dopamine neurons. The pathogenesis of PD is poorly understood, though misfolded and/or aggregated forms of the protein α-synuclein have been implicated in several neurodegenerative disease processes, including neuroinflammation and astrocyte activation. Astrocytes in the midbrain play complex roles during PD, initiating both harmful and protective processes that vary over the course of the disease. However, despite their significant regulatory roles during neurodegeneration, the cellular and molecular mechanisms that promote pathogenic astrocyte activity remain mysterious. Here, we show that α-synuclein preformed fibrils (PFFs) induce pathogenic activation of human midbrain astrocytes, marked by inflammatory transcriptional responses, downregulation of phagocytic function, and conferral of neurotoxic activity. These effects required the necroptotic kinases RIPK1 and RIPK3, but were independent of MLKL and necroptosis. Instead, both transcriptional and functional markers of astrocyte activation occurred via RIPK-dependent activation of NF-κB signaling. Our study identifies a previously unknown function for α-synuclein in promoting neurotoxic astrocyte activation, as well as new cell death-independent roles for RIP kinase signaling in the regulation of glial cell biology and neuroinflammation. Together, these findings highlight previously unappreciated molecular mechanisms of pathologic astrocyte activation and neuronal cell death with implications for Parkinsonian neurodegeneration.
Combining conventional QTL analysis and whole-exome capture-based bulk-segregant analysis provides new genetic insights into tuber sprout elongation and dormancy release in a diploid potato population
Authors: Sharma Sanjeev Kumar , McLean Karen , Colgan Richard J. , Rees Debbie , Young Stephen , Sønderkær Mads , Terry Leon A. , Turnbull Colin , Taylor Mark A. , Bryan Glenn J. 
Published: July 2021

AbstractTuber dormancy and sprouting are commercially important potato traits as long-term tuber storage is necessary to ensure year-round availability. Premature dormancy release and sprout growth in tubers during storage can result in a significant deterioration in product quality. In addition, the main chemical sprout suppressant chlorpropham has been withdrawn in Europe, necessitating alternative approaches for controlling sprouting. Breeding potato cultivars with longer dormancy and slower sprout growth is a desirable goal, although this must be tempered by the needs of the seed potato industry, where dormancy break and sprout vigour are required for rapid emergence. We have performed a detailed genetic analysis of tuber sprout growth using a diploid potato population derived from two highly heterozygous parents. A dual approach employing conventional QTL analysis allied to a combined bulk-segregant analysis (BSA) using a novel potato whole-exome capture (WEC) platform was evaluated. Tubers were assessed for sprout growth in storage at six time-points over two consecutive growing seasons. Genetic analysis revealed the presence of main QTL on five chromosomes, several of which were consistent across two growing seasons. In addition, phenotypic bulks displaying extreme sprout growth phenotypes were subjected to WEC sequencing for performing BSA. The combined BSA and WEC approach corroborated QTL locations and served to narrow the associated genomic regions, while also identifying new QTL for further investigation. Overall, our findings reveal a very complex genetic architecture for tuber sprouting and sprout growth, which has implications both for potato and other root, bulb and tuber crops where long-term storage is essential.
Base-controlled chemoselectivity: direct coupling of alcohols and acetonitriles to synthesise α-alkylated arylacetonitriles or acetamides
Authors: Li Chen , Bai Liang , Ge Min-Tong , Xia Ai-Bao , Wang Ying , Qiu Yuan-Rui , Xu Dan-Qian 
Published: July 2021

[Cp*IrCl2]2 with a phosphine-free ligand α,α,α-terpyridine shows high catalytic performance in chemodivergent synthesis of α-alkylated arylacetonitriles in the presence of K2CO3 and α-alkylated acetamides in the presence of tBuOK, respectively.
Iterative attribute augmentation network for face image super resolution
Authors: Teng Zi , Yu Xiaosheng , Wu Chengdong 
Published: July 2021

AbstractAlthough recently a series of deep‐learning based methods have promoted the performance of face super resolution (FSR), most of these methods cannot recover essential face attributes accurately, especially to super‐resolve a very low‐resolution (LR) face image ( pixels) to its () high‐resolution (HR) version. To address this issue, a novel alternating optimisation algorithm to estimate facial attributes and restore facial images in a single network is presented . Specifically, two convolutional neural modules (denoted as Restorer and Corrector) are constructed and these two modules are alternated repeatedly to form an end‐to‐end trainable network. The Restorer module reconstructs face images based on estimated facial attributes, while the Corrector module corrects the estimated attributes with the help of restored face image. Since Corrector can utilise information from previous estimated attributes and the FSR image, the estimated attributes are iteratively corrected and gradually approach the ground truth. Moreover, a new attribute transformation scheme is designed to introduce attribution information into Restorer, in which the facial attribute vectors as the control conditions can guide the face image restoration explicitly. By conducting extensive experiments on the well‐known CelebA dataset, it is demonstrated that the proposed method can provide superior FSR performance in both quantitative and qualitative measurements.
Modular DC‐DC auto‐transformer: Topology, operation, and system design
Authors: Chen Yang , Wang Han , Zhu Miao , Liu Ming , Cai Xu 
Published: July 2021

AbstractHere, a modular DC‐DC auto‐transformer is proposed with a developed scheme of two‐port submodules. The proposed two‐port submodule is composed of six switches and single capacitor, and it is featured with three general working states. In the proposed DC‐DC auto‐transformer, all two‐port submodules are connected in the input‐series‐output‐series structure. Then the general primary and secondary ports of the submodules of the proposed DC‐DC auto‐transformer are connected in series to achieve the auto‐transformer operation capability. All submodule capacitors are actively connected in the transformer to form the primary and secondary side voltages. The capacitor utilization is increased therefore. Different voltage ratios can be achieved by arranging different submodule numbers in each working state simultaneously. The volume is reduced due to the developed two‐port submodule which eliminates of AC transformers. Detailed topology, working principles, modulation method, and control strategy are provided in the paper. Voltage and current fluctuations are analysed, and the system parameter design method is proposed in detail. Both a simulation system with 40 submodules and a down‐scaled experimental system with three submodules were set up and verified the proposed approach.
Retrospective Cross-sectional Analysis of Factors Associated with Asthma in a Pediatric Cohort from Turkey
Authors: Severcan Ezgi Ulusoy , Bal Cem Murat , Tanac Remziye , Gulen Figen , Demir Esen 
Published: July 2021

AbstractBackground It is difficult to identify young children at increased risk of developing asthma amongst those with recurrent wheezing. In this study, we aimed to determine factors associated with asthma in Turkish children.Methods We performed a retrospective cross-sectional analysis on factors associated with asthma in 651 children (200 girls, 451 boys) admitted for recurrent wheezing.Results Amongst all included children, asthma frequency was 57.7%. Maturity, consanguinity, family income, passive smoking, father’s, siblings’ asthma were not found to be associated with asthma. Factors associated with asthma were: family’s, parents’, siblings’ atopy, family’s, mother’s asthma, allergic rhinitis and atopic dermatitis, respiratory symptoms between wheezing attacks. The sensitivity (SN) of the modified asthma predictive index (mAPI) was 59.2% with a specificity (SP) of 91.3%, positive predictive index (PPI) of 65.1% and negative predictive index (NPI) of 82.3%. The SN of the modified Prevention and Incidence of Asthma and Mite Allergy (PIAMA) score was 22.9% with a SP of 89.2%, PPI of 84.6%, and NPI of 42.2%. Adjusted odds ratio for mAPI was 12.9, and for the modified PIAMA score 4.Conclusion Our analysis confirmed previously described factors associated with asthma. Although the SN is limited, the mAPI and PIAMA risk scores can be used to predict asthma in Turkish children. Differential diagnoses and overlaps with other chronic pediatric diseases such as immunodeficiencies need to be carefully excluded when confirming the diagnosis asthma.
Phytogenic Compounds for Enhancing Intestinal Barrier Function in Poultry–A Review
Authors: Latek Urszula , Chłopecka Magdalena , Karlik Wojciech , Mendel Marta 
Published: July 2021

AbstractAfter the European Union ban of antibiotic growth promoters, works on different methods of improving gut health have intensified. The poultry industry is struggling with problems that were previously controlled by antibiotic growth promoters, therefore the search for optimal solutions continues. Simultaneously, there is also increasing social pressure to minimize the use of antibiotics and replace them with alternative feed additives. A variety of available alternatives is considered safe by consumers, among which phytogenics play a significant role. However, there are still some limitations that need to be considered. The most questionable are the issues related to bioavailability, metabolism of plant derivatives in birds, and the difficulty of standardizing commercial products. There is still a need for more evidence-based recommendations for the use of phytogenics in livestock. On the other hand, a positive influence of phytogenic compounds on the health of poultry
has been previously described by many researchers and practical application of these compounds has auspicious perspectives in poultry production. Supplementation with phytogenic feed additives has been shown to protect birds from various environmental threats leading to impaired intestinal barrier function. Phytogenic feed additives have the potential to improve the overall structure of intestinal mucosa as well as gut barrier function on a molecular level. Recognition of the phytogenicsʼ effect on the components of the intestinal barrier may enable the selection of the most suitable ones to alleviate negative effects of different agents. This review aims to summarize current knowledge of the influence of various phytogenic constituents on the intestinal barrier and health of poultry.
Benzoylcyclopropane Derivatives from Hypoxis hemerocallidea Corms
Authors: Zulfiqar Fazila , Pandey Pankaj , Tripathi Siddharth K. , Ali Zulfiqar , Chittiboyina Amar G. , Khan Ikhlas A. 
Published: July 2021

AbstractTwo monobenzoylcyclopropane (hypoxhemerol A (1) and hypoxhemeroloside G (2)) and three dibenzoylcyclopropane (hypoxhemerol B (3), hypoxhemeroloside H (4), and hypoxhemeroloside I (5)) derivatives were isolated from the hydro-alcoholic extract of Hypoxis hemerocallidea corms. This is the first instance where benzoylcyclopropane analogs were isolated from any natural source. Structure elucidation was mainly based on 1D- and 2D-NMR and HRESIMS data. The absolute configuration (2R, 4R) of 1 was determined via NOESY NMR and experimental and calculated ECD data analyses. Compounds 1–5 and 11 recently reported metabolites (hypoxoside, obtuside A, interjectin, acuminoside, curcapicycloside, and hypoxhemerolosides A – F) were screened for in vitro antimicrobial activity against various bacterial and fungal strains. Curcapicycloside and acuminoside exhibited antibacterial activity against Escherichia coli with 78 and 79% inhibition at 20 µg/mL, respectively. Hypoxhemeroloside A showed mild antifungal activity against Cryptococcus neoformans with 63% inhibition at 20 µg/mL.
Monströser solitärer fibröser Tumor der Pleura
Authors: Prisadov Georgi , Hendrix Holger , Kamlak Vladimir , Welcker Katrin 
Published: July 2021

ZusammenfassungLokalisierte, solitäre fibröse Tumoren sind eine seltene Entität und finden sich meist im Thorax, können aber auch in Leber, Haut, Meningen, am Peritoneum und Perikard auftreten. Aufgrund des vielgestaltigen mikromorphologischen Erscheinungsbildes ist die Sicherung der Diagnose oftmals schwierig. Entscheidend ist die histopathologische Begutachtung mit differenzierter Immunhistochemie. Die chirurgische Resektion der lokalisierten, solitären fibrösen Tumoren ist die Therapie der Wahl. Die Rezidiv- und Metastasenrate ist abhängig von der histologischen Dignität insgesamt sehr gering. Kontinuierliche Nachsorgeuntersuchungen sind indiziert.Wir berichten über eine 76-jährigen Patientin mit einem monströsen solitären fibrösen Tumor der Pleura, die seit einigen Wochen über Belastungsdyspnoe und stechende Schmerzen rechts thorakal klagte. Computertomografisch zeigte sich eine heterogene Raumforderung mit ca. 22 cm Durchmesser im Mittel- und Unterfeld des Thorax mit Kompression des Zwerchfells und des Mediastinums. Der Tumor wurde über eine doppelte Thorakotomie im 5. und im 8. Interkostalraum vollständig reseziert. Intraoperativ zeigte sich der Tumor zum Mittellappen gestielt. Um den Tumor vollständig zu entfernen, wurde zusätzlich eine Keilresektion aus dem Mittellappen durchgeführt. Der Tumor wog 2,4 kg. Die Diagnose eines solitären fibrösen Tumors der Pleura wurde histologisch und immunhistochemisch gestellt. Postoperativ hat sich die Lunge vollständig entfaltet. Bei der 3-monatigen Nachuntersuchung zeigte sich kein Nachweis eines Rezidivs. Die klinische Symptomatik, Diagnosestellung, Therapieoptionen, Rezidivrisiko und die Prognose dieser Tumore werden aufgezeigt und entsprechend der Literatur diskutiert.
Effect Modification of Cardiorespiratory Fitness, Obesity, and
Physical Activity in Adults
Authors: do Socorro Morais Pereira Simoes Maria , Wehrmeister Fernando Cesar , Romiti Marcello , de Toledo Gagliardi Antonio Ricardo , Arantes Rodolfo Leite , Dourado Victor Zuniga 
Published: July 2021

AbstractWe investigated if cardiorespiratory fitness modifies the association between
obesity and the level of physical activity. In this cross-sectional study, we
analyzed data from 746 adults, free of diagnosed cardiorespiratory or locomotor
diseases. We analyzed sociodemographic and clinical information, cardiovascular
risk factors, cardiorespiratory fitness, anthropometry, and level of physical
activity (time spent in moderate-to-vigorous physical activity). Those that
spent more time in moderate-to-vigorous physical activity were younger, male,
with lower body mass index, without self-reported arterial blood hypertension,
diabetes and dyslipidemia, non-smokers, and presented with better
cardiorespiratory fitness. The linear regression coefficients showed that
cardiorespiratory fitness changes according to the level of physical activity
and body mass index (obesity in low cardiorespiratory fitness: β 6.0,
p=0.213, 95%CI-3.5 to 15.6; in intermediate cardiorespiratory
fitness: β 6.3, p=0.114, 95%CI-1.5 to 14.2; in high
cardiorespiratory fitness: β-6.3, p=0.304, 95%CI-18.4 to
5.8). This effect modification trend was present after adjusting the model by
covariates. Cardiorespiratory fitness potentially modifies the association
between body mass index and the level of physical activity. It should be
routinely assessed to identify persons with overweight/ obesity with
low/ intermediate cardiorespiratory fitness to prescribe individualized
training.
A Simple, Readily Accessible, and Effective Apparatus for the ­Photoisomerization of cis-Cyclooctenes to trans-Cyclooctenes
Authors: Pickel Thomas C. , Genung Nathan E. , Guckian Kevin M. , Shi Xianglin 
Published: July 2021

AbstractA simple, cost effective, and readily accessible apparatus for the photoisomerization of cis-cyclooctenes to trans-cyclooctenes is described. Utilizing only FEP tubing, aluminum vent pipe, a household germicidal lamp, and a flash chromatography system, trans-cyclooctenes can be prepared in good yield.
Investigation on the Stoichiometry of Carbon Dioxide in Isotope-Exchange Reactions with Phenylacetic Acids
Authors: Audisio Davide , Talbot Alex , Sallustrau Antoine , Goudet Amélie , Taran Frédéric 
Published: July 2021

AbstractThe functionalization of carbon dioxide (CO2) as a C1 building block has attracted enormous attention. Carboxylation reactions, in particular, are of major interest for applications in isotope labeling. Due to the inexpensive nature of CO2, information about its stoichiometric use is generally unavailable in the literature. Because of the rarity and limited availability of CO2 isotopomers, this parameter is of concern for applications in carbon-isotope labeling. We investigated the effects of the stoichiometry of labeled CO2 on carbon isotope exchange of phenyl­acetic acids. Both thermal and photocatalytic procedures were studied, providing insight into product outcome and isotope incorporation. Preliminary results on isotope-dilution effects of carbonate bases in photocatalytic carboxylation reactions have also been obtained.
Cor Triatriatum Dexter with Noncompaction of Right Ventricle and Complete Heart Block: An Unholy Trinity
Authors: Bhandari Monika , Pradhan Akshyaya , Vishwakarma Pravesh , Sethi Rishi 
Published: July 2021

AbstractCor triatriatum (CT) or a triatrial heart is a rare congenital anomaly in which one of the atrial chambers is divided by a fibromuscular membrane. Of the two variants, CT dexter (right-sided CT) is still further rare than CT sinister (left-sided CT). Although CT sinister presents with features of left heart obstructive disease mimicking mitral stenosis, CT dexter is usually asymptomatic and is found incidentally on imaging. Here, we present a patient with an unusual case of complete heart block who was found to have CT dexter along with right ventricular noncompaction on imaging.
Long-Term Seizures Outcome in Juvenile Myoclonic Epilepsy (JME): A Retrospective Cohort Study in an Indian Population
Authors: Viswanathan Lakshminarayanapuram Gopal , Mundlamuri Ravindranadh Chowdary , Raghavendra Kenchaiah , Asranna Ajay , Chatterjee Aparajita , Gautham Bhargava , Satishchandra Parthasarathy , Sinha Sanjib 
Published: July 2021

Abstract
Objectives Juvenile myoclonic epilepsy (JME) is the most prevalent form of generalized epilepsy in the community. Though the response to medication is good in most patients, long-term medication usage may lead to poor compliance, side effects, and other epilepsy-related social issues. The aim of this study was to describe the demography, clinical features, and therapeutic response in patients with JME who have had at least 10 years of duration of epilepsy.
Materials and Methods A retrospective study of 56 patients (M:F=26:30; mean age: 14.5 ± 5.1 years) with JME attending neurological services in a tertiary care neurological center in South India with more than 10 years disease duration was performed.
Results All patients had myoclonic jerks, 94% had generalized tonic–clonic seizure, and only 7.1% had features of absence seizures. Seizures were controlled in 60% of cases at the end of 10 years of epilepsy. Thirty-eight patients (67.8%) were on valproate. Control of seizures at the end of the first and fifth years of epilepsy, frequent myoclonic jerks at the onset, and treatment with valproate in the first year of disease resulted in controlled seizures at the end of 10 years. No patient was off medications at the end of 10 years of epilepsy or at the last follow-up.
Conclusion About 40% of our patients continued to have seizures even 10 years after the onset of epilepsy. Regular and long-term medication may be required in JME. Early control of seizures, response to valproate, and frequent myoclonia at onset were found to confer better prognosis.