bluuebunny/crossref_metadata_2025_split huggingface dataset:
Integrated Analysis of Helicobacter Pylori-Related Prognostic Gene Modification Patterns in the Tumour Microenvironment of Gastric Cancer
Authors: Wang Ye , Zheng kaitian , Wang Jiancheng , Wang congjun , Chen Junqiang 
Published: December 2021

Abstract
Background
Helicobacter pylori (HP) infection is one of the leading causes of gastric cancer (GC). However, the interaction between HP and the TME, and its carcinogenic mechanism remains unknown.
Methods
We screened 28 HP-related prognostic genes based on HP infection-related gene markers and HP infection sample datasets (GSE6143 and GSE60662). We then constructed an HPscore system by using the principal component analysis algorithm and successfully quantified the HP modification characteristics of a single GC sample. In addition, we comprehensively analysed the relationship between the HPscore and the clinical characteristics of patients with GC, the immune cell infiltration characteristics of the TME and stemness.
Results
We successfully identified 28 HP-related prognostic genes that accurately classified the GC population. There are significant differences in survival between different subgroups(high-, low-risk and cluster_1,2). Thereafter, we constructed the HPscore system to systematically evaluate the modification characteristics of the 28 HP-related prognostic genes, and the internal and external validation of the HPscore system suggested similar results: the overall survival rate in the high -HPscore group was poor, and immunological surveillance was reduced, whereas the low-HPscore group had a survival advantage, and was related to the inflammatory response. HPscore was also strongly correlated with the tumour stage, TME cell infiltration and stemness.
Conclusions
HP-modified characteristics play a crucial role in the TME and tumourigenesis. HPscore evaluation of a single tumour sample can help identify the TME characteristics and the carcinogenic mechanism of GC patients infected with HP, based on which personalised treatment can be administered.
Validation of the Hospital Frailty Risk Score among older adults receiving mechanical ventilation
Authors: Sy Eric , Kassir Sandy , Mailman Jonathan F , Sy Sarah Lauren 
Published: December 2021

Abstract
Background:Older adults are increasingly being admitted to intensive care units, with frailty recognized as a risk factor for worse outcomes. The Hospital Frailty Risk Score (HFRS) was developed for use in administrative databases of older adults, but it has not yet been well-validated for critically ill patients. The objective of this study was to validate the HFRS to predict prolonged hospitalization, in-hospital mortality, and 30-day emergency hospital readmissions in critically ill patients.Methods:We selected index hospitalizations of older adults (≥75 years old) receiving mechanical ventilation, using the United States Nationwide Readmissions Database from January 1, 2016 to November 30, 2018. Frailty risk was determined by the HFRS using International Classification of Diseases, Tenth Revision Clinical Modification (ICD-10-CM) codes, and further subcategorized into low (score <5), intermediate (score 5-15), and high (score >15) risk for frailty. We evaluated the HFRS to predict prolonged hospitalization, in-hospital mortality, and 30-day emergency hospital readmissions, using multivariable logistic regression after adjustment for patient and hospital characteristics. Model performance was assessed using the c-statistic, Brier score, and calibration plots.Results:Among the 649,330 weighted index hospitalizations in the cohort, 50.0% were female, the median (interquartile range [IQR]) age was 81 (78-86) years old, and the median (IQR) HFRS was 10.8 (7.7-14.5). Among the cohort, 9.5%, 68.3%, and 22.2% were subcategorized as low, intermediate, and high risk for frailty, respectively. After adjustment, patient hospitalizations with high frailty risk were associated with increased risks of prolonged hospitalization (adjusted odds ratio [aOR] 5.59 [95% confidence interval [CI] 5.24-5.97], c-statistic 0.694, Brier score 0.216) and 30-day emergency hospital readmissions (aOR 1.20 [95% CI 1.13-1.27], c-statistic 0.595, Brier score 0.162), compared to low frailty risks. Conversely, high frailty risk using the HFRS was inversely associated with in-hospital mortality (aOR 0.46 [95% CI 0.45-0.48], c-statistic 0.712, Brier score 0.214). Calibration plots demonstrated good calibration for the adjusted analyses.Conclusions:The HFRS is associated with prolonged hospitalization and 30-day readmission in older adults receiving mechanical ventilation. Further research is necessary to develop frailty scores that accurately and intuitively predict mortality in critically ill patients.
Visual outcomes after bilateral implantation of Versario intraocular lenses – six month follow-up
Authors: Podboraczynska-Jodko Karolina , Lubiński Wojciech 
Published: December 2021

Abstract
PurposeTo study visual outcomes after implantation of Versario intraocular lenses (IOLs) Patients and MethodsIn 20 selected patients (40 eyes) uneventful, bilateral cataract surgeries with implantation of trifocal Versario (IOLs) were performed. Designprospective case series. Six months after surgery the following parameters were analysed: binocular uncorrected visual acuities (log MAR): for distance (UDVA) at 4 m, for intermediate (UIVA) at 60, 70, 80 cm and for near (UNVA) at 40 cm, photopic defocus curve, mesopic and photopic contrast sensitivities (CSs), spectacle independence, visual function test questionnaire (modified VFQ-25), subjective symptoms and postoperative complications. ResultsSix months after surgery, the mean of binocular visual acuities were as follows: UDVA: -0.12 ± 0.08; UIVA (60 cm): +0.07 ± 0.10, (70 cm): +0.05 ± 0.11, (80cm): +0.07 ± 0.09; UNVA (40cm): +0.12 ± 0.08. CSs in mesopic and scotopic conditions for distance and photopic for near were between normal range of age- match healthy control. All patients were spectacle independent. General satisfaction was high and equal 9.37±0.83. No severe glare and halo were detected. There were no postoperative complications. ConclusionsThe bimanual MICS with the Versario lenses implantation was a safe and effective method for patients who want to be spectacle independent at different distances.
Novel Immune Subtypes Identification of HER2 Positive Breast Cancer Based On Immunogenomic Landscape
Authors: Huang Lingli , Liu Xin , Li Li , Wang Lei , Wu Nan , Liu Zhixian 
Published: December 2021

Abstract
Background: HER2 positive BC is heterogeneous. But few studies discussed the classification of HER2 positive BC based on immune-related signatures.Methods: Using two publicly BC genomics datasets, we classified HER2 positive BC based on 33 immune-related signatures and used unsupervised machine learning methods to predict and perform the classification.Results: We grouped three HER2 positive BC subtypes that we called Immune-High (IM-H), Immune-Medium (IM-M), and Immune-Low (IM-L), and manifested this categorization was predictable, duplicable and reliable by analyzing another dataset. Compared to other subtypes, IM-H had a higher immune cell infiltration level and stronger anti-tumor immune activities, as well as better clinical survival outcome. Besides these signatures, there were some cancer-related pathways which were hyperactivated in IM-H, including cytokine-cytokine receptor interactions, antigen processing and presentation pathways, natural killer cell-mediated cytotoxicity, Th1 and Th2 cell differentiation, chemokine signaling pathway, Th17 cell differentiation, B and T cell receptor signaling, NF-kappa B signaling, PD-L1 expression and PD-1 checkpoint pathway in cancer, TNF signaling, IL-17 signaling, NOD-like receptor signaling and Toll-like receptor signaling. By contrast, IM-L showed depressed immune-related signatures and enhanced activation of lycosylphosphatidylinositol-anchor biosynthesis and mismatch repair. Moreover, we discovered a gene co-expression network focused on eight transcription factor genes (EOMES, TBX21, GFI1, IRF4, POU2AF1, CIITA, FOXP3 and TOX) and one tumor suppress gene (PRF1), which were closely related with tumor immune.Conclusion: We identified three HER2 positive BC subtypes based on immune-related signatures, which had potential clinical implications and promoted the optimal stratification of HER2 positive BC responsive to immunotherapy.
Advanced Healthcare Services Leading to Relax Natural Selection May Have Been Contributing to Global and Regional Increase of Dementia Incidence
Authors: You Wenpeng , Henneberg Renata , Henneberg Maciej 
Published: December 2021

Abstract
BackgroundAgeing and genetic traits can only explain the increasing dementia incidence partially. Advanced healthcare services allowing dementia patients to survive natural selection (die of dementia) and pass their genes onto their next generation. MethodsCountry-speciﬁc estimates of dementia incidence rates (all ages and 15-49 years old), Biology State Index (Is) expressing reduced natural selection, ageing indexed by life expectancy e(65), GDP PPP and urbanization were obtained for analysing the global and regional correlations between reduced natural selection and dementia incidence rate with SPSS v. 27. Results Worldwide, both Is significantly, but inversely, correlated to dementia incidence rates for both populations in bivariate correlations. These relationships remained in the populations of all ages and 15-49 years old regardless of the competing contributing effects from ageing, GDP and urbanization in partial correlation model. Enter multiple linear regression showed that Is was the significant predictor of dementia incidence in populations of all ages and 15-49 years old. Subsequently, Is was selected as the variable having the greatest influence on dementia incidence in stepwise multiple linear regression. Is corelated to dementia incidence more strongly in developed population groupings. ConclusionsWorldwide, reduced natural selection may be another the significant contributor to the increasing dementia incidence with special regard to developed population.
Efficacy and Safety of De-Escalation of Anti-Platelet Therapy after Percutaneous Coronary Intervention in Patients with Acute Coronary Syndrome: A Meta-Analysis of Randomized Clinical Trials
Authors: Bai Nan , Ma Ying , Niu Ying , Zhong Pengyu , Shang Yaosheng , Wang Zhilu 
Published: December 2021

Abstract
Purpose: This meta-analysis was conducted to study the efficacy and safety of switching from potent P2Y12 inhibitors to clopidogrel, because there is no definite conclusion on the strategy.Method: We conducted a systematic review and meta-analysis of patients with acute coronary syndromes (ACS) undergoing percutaneous coronary intervention, and compared the efficacy and safety of de-escalation or not of anti-platelet therapy. The relevant randomized controlled trials were included by searching several databases. Net adverse clinical events were identified as the composite endpoint, which was defined as a composite of cardiovascular death, myocardial infarction, revascularization, stroke and bleeding at 12 months after ACS. The efficacy endpoints were cardiovascular death, myocardial infarction, revascularization, stroke, all-cause death and stent thrombosis. Bleeding was designed as the safety endpoint. The relative risk (RR) and 95% confidence intervals (CI) of endpoint events were calculated by the fixed effects model. Result: Six randomized controlled trials with 7627 patients met inclusion criteria. There were significant differences in the risk of net adverse clinical events (RR 0.67, CI 0.58–0.78, P < 0.00001), and bleeding endpoint (0.61, 0.52–0.71, P < 0.00001) between the two groups. However, there were no significant differences in the risk of all efficacy endpoints.Conclusions: The strategy of de-escalation from prasugrel/ticagrelor to clopidogrel can reduce the incidence of net adverse clinical events and bleeding events in patients with ACS undergoing percutaneous coronary intervention (Registered by PROSPERO at July 04 2021, ID 265782).
The Detection of Wetlands And Wetland Fragmentation Using Sentinel 1 And 2 Imagery: The Example of Southern Nigeria
Authors: Garba Sani Idris , Ebmeier Susanna , Bastin Jean-François , Mollicone Danilo , Holden Joseph 
Published: December 2021

Abstract
Wetland ecosystems play key roles in global biogeochemical cycling, but their spatial extent and connectivity is often not well known. Here, we describe an approach suitable for application on a 1000 km scale using Sentinel-1 and Sentinel-2 imagery, exploiting the implementation of Random Forest algorithm in Google Earth Engine. The approach was used to detect the spatial coverage of wetland types focusing on the case of southern Nigeria, thought to be one of the most wetland-rich areas of Africa. We compiled 1050 wetland and non-wetland control points for algorithm training and validation, primarily from visual interpretation of high-resolution (<1 m pixel) imagery. This allowed us to establish the relative importance of 18 input channels derived from Sentinel-1 polarimetric and Sentinel-2 indices for classification of wetland. We estimate that the swamps, marshes, mangroves, and shallow water wetlands of southern Nigeria cover 29,900 km² with 2% uncertainty of 460 km². We found larger mangrove and smaller marsh extent than suggested by earlier, coarser spatial resolution studies. Average continuous wetland patch areas were 120 km², 11 km², 55 km² and 13 km² for mangrove, marsh, swamp, and shallow water respectively. Our final map with 10 m pixels also captures small patches of wetland, with 20% of wetland patches being <1 km2; these were clustered around urban centres, suggesting anthropogenic wetland fragmentation. Our approach can now be used across rest of Africa and globally to detect wetlands and wetland change which, in turn, will be crucial for improved land-surface climate models and wetland conservation.
Can a Whole-Body Vibration Program Potentialize the Benefits of a Psychomotor Intervention Program in Community-Dwelling Older Adults At Risk of Falling? A Randomized Controlled Trial
Authors: Rosado Hugo , Pereira Catarina , Bravo Jorge , Carvalho Joana , Raimundo Armando 
Published: December 2021

Abstract
Background

To evaluate the effects of two interactive cognitive-motor programs in processing speed, lower-body strength, and body composition in community dwellings at risk of falling.
Methods

Forty-eight community dwellings (75.0 ± 5.4 years) completed this randomized controlled trial, were allocated into three groups: 1) experimental group 1 (EG1: psychomotor intervention program); 2) experimental group 2 (EG2: combined program [psychomotor intervention program + whole-body vibration]); and 3) control group (kept their daily life routines). Participants were assessed at baseline, at post-24-week intervention, and after a 12-week no-intervention follow-up.
Results

Significant improvements were induced by EGs programs in processing speed, lower-body strength, and bone mass (p < 0.05). The treatment effect was similar in both EGs in processing speed and lower-body strength, and higher in bone mineral content and density within EG2. The number of falls decreased by 44.2% in EG1 and 63% in EG2 (p < 0.05). After the follow-up, improvements in processing speed were maintained, particularly in EG2, but were reversed in lower-body strength in both EGs, as were in bone mineral content and density, particularly within EG2 (p < 0.05).
Conclusions

Both interactive cognitive-motor programs were accepted and well tolerated by the participants, inducing similar improvements in cognitive and physical functions and decreased the fall rate. Additionally, the combined program led to additional benefits in bone mass. This evidenced that both programs were effective for fall and injury prevention.
Trial registration:

ClinicalTrials.gov Identifier: NCT03446352, registered on 26/02/2018.
An Assessment of Recreational Functions of Botanical Parks in Urban Ecosystems
Authors: Akdeniz Nilufer Seyidoglu , Yener S. Doganay 
Published: December 2021

Abstract
In the changing world, pandemics have entered human life and people want to relieve the stress and relax that these new living conditions they meet. Parks in the urban ecosystem are one of the indispensable elements in this sense. In this context, while botanical parks contribute to the urban ecosystem, they also meet the recreational needs of people.In this study, a survey was carried out for assessing the recreational quality of Bakırköy Botanical Park in Bakırköy, Istanbul, which is Turkey’s most important metropolitan city. The results of the survey reveal that the users perceived Bakırköy Botanical Park as an urban park, and the findings reveal that young users (26-35 years old) visited the park mostly within 1-3 hours. In addition to being a park to get away from the crowd (78.00%) in terms of comfort and image, the presence of children’s playgrounds is also considered an important recreational function for users (66.80%). Although the park has some deficiencies in terms of reinforcement elements and negligence, observations exhibited that it cannot fulfill its education and research functions adequately as a botanical park. Hence, the plants in the park must have labels. It would be an appropriate approach to make the necessary arrangements for Bakırköy Botanical Park to maintain both its recreational and educational functions, and to consider the protection-use balance in the arrangements.
Gastrointestinal Parasitosis and Anemia Monitoring in the Child Population of Villa Clarín, Colombia
Authors: Vásquez Rubén Darío Macías , Vesga Darling María Guzmán , Rivaldo María Ripoll , Arias Elkyn Rafael Lugo , Arias José Luis Lugo , Pacheco Mario de la Puente 
Published: December 2021

Abstract
BackgroundThere is limited non-anecdotal literature on the effectiveness of health policies and the prevalence of childhood anemia in rural communities in the Colombian Caribbean region. The objective of the following study is to report a parasitological and hematological episode of 94 children belonging to the child population of Villa Clarín, Colombia, and reinforce the disposable literature of local care reports.Case presentationThis article studies an event of intestinal parasitosis prevalence and anemia in 94 children between 1 and 11 years of age, grouped into three age ranges of 1 to 3 years, 4 to 6 years, and 7 to 11 years of age respectively, from the rural community of Villa Clarín, Colombia. Blood samples were obtained by venipuncture and processed through a complete blood count. The feces were collected by spontaneous evacuation and analyzed using a spontaneous sedimentation technique. The prevalence of intestinal parasitosis was 96.81%. ConclusionsEight of the 91 children in whom intestinal parasites were detected had anemia while 29 were above the normal levels of hemoglobin. An association was found with E. histolytica / dispar, but not with A. lumbricoides, T. trichiura, S. stercolaris, Uncinaria, Taenia sp, H. nana, H. diminuta, G. lamblia, T. hominis or B. hominis. The high prevalence of intestinal parasitosis and the anemia levels indicate deficiencies in environmental sanitation and hygienic-sanitary measures.
Interleukin-6 Differential Expression in Cancer Patients of Different Clinical Stages; a Possible Biomarker of Cancer Progression
Authors: Bello Abosede Obafunke , Obadipe John Abiodun , Adewusi Oluwabanke Temitope , Ayanshina Anthony Oluwamuyiwa 
Published: December 2021

Abstract
PurposeThe study investigated interleukin-6 expression pattern across all stages of cancer. The research questions raised in the study were: Is there differential expression of Interleukin-6 across all cancer stages? and what relationship exists between serum interleukin-6 level and cancer stage?Methods The prospective case-control study comprised sixty two (62) purposively selected cancer participants across all stages and age range 18 years to 72years as well equal number of healthy volunteers from two medical centres in Nigeria. Three milliliters (3mls) of blood samples was collected intravenously from the participants and centrifuged after 30 minutes of collection at 3000rpm for 10 minutes to obtain serum. The serum level of Interleukin-6 was determined spectrophotometrically by Enzyme linked immunosorbent assay (ELISA). Data obtained were expressed as mean and standard error of the mean. One way Analysis of variance and t-test were employed to test for significance difference between the groups and the significant level was considered at P< 0.05. ResultsFindings from the study revealed significant (P< 0.05) higher mean serum interleukin-6 levels in stage IV cancer participants as compared to other disease stages. In the same way, significant higher mean Interleukin-6 level of stage III cancer participants as compared to that of stage I cancer participants was observed. Furthermore, the study revealed a significant correlation (P< 0.01) between serum Interleukin 6 concentration and cancer stage.Conclusion Serum interleukin-6 had differential expression in cancer patients at advanced clinical stage as compared to that of early disease stage.
Invertebrate DNA Methylation and Gene Regulation
Authors: Dixon Groves , Matz Mikhail 
Published: December 2021

Abstract
BackgroundAs human activity alters the planet, there is a pressing need to understand how organisms adapt to environmental change. Of growing interest in this area is the role of epigenetic modifications, such as DNA methylation, in tailoring gene expression to fit novel conditions. Here, we reanalyzed nine invertebrate (Anthozoa and Hexapoda) datasets to validate a key prediction of this hypothesis: changes in DNA methylation in response to some condition correlate with changes in gene expression. ResultsWhile we detected both differential methylation and differential expression, there was no simple relationship between these differences. ConclusionIf changes in DNA methylation regulate invertebrate transcription, the mechanism does not follow a simple linear relationship.
Image Mosaic Research and Realization Based on LoFTR Algorithm
Authors: Tian Aikui , Wang Kangtao , zhang liye , Wei Bingcai 
Published: December 2021

Abstract
Aiming at the problem of inaccurate extraction of feature points by the traditional image matching method, low robustness, and problems such as diffculty in inentifying feature points in area with poor texture. This paper proposes a new local image feature matching method, which replaces the traditional sequential image feature detection, description and matching steps. First, extract the coarse features with a resolution of 1/8 from the original image, then tile to a one-dimensional vector plus the positional encoding, feed them to the self-attention layer and cross-attention layer in the Transformer module, and finally get through the Differentiable Matching Layer and confidence matrix, after setting the threshold and the mutual closest standard, a Coarse-Level matching prediction is obtained. Secondly the fine matching is refined at the Fine-level match, after the Fine-level match is established, the image overlapped area is aligned by transforming the matrix to a unified coordinate, and finally the image is fused by the weighted fusion algorithm to realize the unification of seamless mosaic of images. This paper uses the self-attention layer and cross-attention layer in Transformers to obtain the feature descriptor of the image. Finally, experiments show that in terms of feature point extraction, LoFTR algorithm is more accurate than the traditional SIFT algorithm in both low-texture regions and regions with rich textures. At the same time, the image mosaic effect obtained by this method is more accurate than that of the traditional classic algorithms, the experimental effect is more ideal.