bluuebunny/crossref_metadata_2025_split huggingface dataset:
Exosomal miRNA profiling from H5N1 avian influenza virus-infected chickens
Authors: Hong Yeojin , Truong Anh Duc , Lee Jiae , Vu Thi Hao , Lee Sooyeon , Song Ki-Duk , Lillehoj Hyun S. , Hong Yeong Ho 
Published: March 2021

AbstractExosomes are membrane vesicles containing proteins, lipids, DNA, mRNA, and micro RNA (miRNA). Exosomal miRNA from donor cells can regulate the gene expression of recipient cells. Here, Ri chickens were divided into resistant (Mx/A; BF2/B21) and susceptible (Mx/G; BF2/B13) trait by genotyping of Mx and BF2 genes. Then, Ri chickens were infected with H5N1, a highly pathogenic avian influenza virus (HPAIV). Exosomes were purified from blood serum of resistant chickens for small RNA sequencing. Sequencing data were analysed using FastQCv0.11.7, Cutadapt 1.16, miRBase v21, non-coding RNA database, RNAcentral 10.0, and miRDeep2. Differentially expressed miRNAs were determined using statistical methods, including fold-change, exactTest using edgeR, and hierarchical clustering. Target genes were predicted using miRDB. Gene ontology analysis was performed using gProfiler. Twenty miRNAs showed significantly different expression patterns between resistant control and infected chickens. Nine miRNAs were up-regulated and 11 miRNAs were down-regulated in the infected chickens compared with that in the control chickens. In target gene analysis, various immune-related genes, such as cytokines, chemokines, and signalling molecules, were detected. In particular, mitogen-activated protein kinase (MAPK) pathway molecules were highly controlled by differentially expressed miRNAs. The result of qRT-PCR for miRNAs was identical with sequencing data and miRNA expression level was higher in resistant than susceptible chickens. This study will help to better understand the host immune response, particularly exosomal miRNA expression against HPAIV H5N1 and could help to determine biomarkers for disease resistance.
Switching vaccination schemes for epidemic models with distributed time delay and two types of noise
Authors: Wang X. Y. 
Published: March 2021

AbstractNovel switched HIV/AIDS (human immunodeficiency virus/acquired immune deficiency syndrome) epidemic models with distributed time delay and bounded noise and Gaussian white noise are developed and investigated using stochastic Itô’s lemma and the Lyapunov–Razumikhin method. New criteria depending on these factors are established to confirm that the disease-free equilibrium of the model is stochastically asymptotically stable as the threshold parameter is less than unity, which implies that the disease eventually disappears theoretically. Otherwise, the disease persists weakly. Further, the main results show that the threshold values are related to two types of noise and time delay. Pulse control strategies are then applied to two types of the infected population, the susceptible population, and the infected population, respectively. More precisely, the effects of each control strategy on the stochastic solution of the model are evaluated to justify the relation between control parameters and threshold parameters of the model. In comparison with the basic reproduction number of the model with pulse control, it is easily found that the main results in these references are improved and extended. Finally, four examples are presented to support the main results, and one future research direction is suggested.
Factors affecting women entrepreneurs’ success: a study of small- and medium-sized enterprises in emerging market of Pakistan
Authors: Khan Rizwan Ullah , Salamzadeh Yashar , Shah Syed Zulfiqar Ali , Hussain Mazhar 
Published: March 2021

AbstractIn the present era, women are recognized as successful entrepreneurs through their strong desire, qualities, and capabilities for robust economic development. Due to such an important contribution of women in economic development, we propose to investigate the factors which affect women entrepreneur’s success in Pakistan. Data were collected through structured questionnaires from 181 registered SMEs operating in Pakistan. A conceptual model is developed, while SPSS and AMOS software’s are used for analysis. The results indicate that the internal factors including the need for achievements, risk-taking, and self-confidence and external factors including economic factors and socio-cultural factors have a positive and significant influence on the success of women-owned enterprises. This research recommends Small and Medium Enterprises Development Authority (SMEDA), policymakers, and practitioners to encourage women entrepreneurs to run their businesses for the long term by providing a variety of incentives and supports related to those internal and external factors. Numerous studies have been conducted to test the different factors’ effects on women’s entrepreneurial success, but our study investigated some psychological, cultural, and religious factors that are still almost untouched especially in Pakistan. The current study also contributes to the existing literature through empirical shreds of evidence.
Co-movement of commodity price indexes and energy price index: a wavelet coherence approach
Authors: Kirikkaleli Dervis , Güngör Hasan 
Published: March 2021

AbstractThis research sheds light on the causal link between commodity price indexes, i.e., the Agricultural Raw Materials Price Index, Industry Input Price Index, Metal Price Index, and Energy Price Index, in the global market, using wavelet coherence, Toda–Yamamoto causality, and gradual shift causality tests over the period 1992M1 to 2019M12. Findings from the wavelet power spectrum and partial wavelet coherence reveal that: (1) there was significant volatility in the Agricultural Raw Materials Price Index, Industry Input Price Index, Metal Price Index, and Energy Price Index between 2004 and 2014 at different frequencies; and (2) commodity price indexes significantly caused the energy price index at different time periods and frequencies. It is noteworthy that the outcomes of the Toda–Yamamoto causality and gradual-shift causality tests are in line with the results of wavelet coherence.
Safety and efficacy of stent-assisted coiling for acutely ruptured wide-necked intracranial aneurysms: comparison of LVIS stents with laser-cut stents
Authors: Xue Gaici , Zuo Qiao , Zhang Xiaoxi , Tang Haishuang , Zhao Rui , Li Qiang , Fang Yibin , Yang Pengfei , Hong Bo , Xu Yi , Huang Qinghai , Liu Jianmin 
Published: March 2021

Abstract
Background
To compare the safety and efficacy of LVIS stent-assisted coiling with those of laser-cut stent-assisted coiling for the treatment of acutely ruptured wide-necked intracranial aneurysms.

Methods
Patients with acutely ruptured wide-necked intracranial aneurysms treated with LVIS stent-assisted coiling (LVIS stent group) and laser-cut stent-assisted coiling (laser-cut stent group) were retrospectively reviewed from January 2014 to December 2017. Propensity score matching was used to adjust for potential differences in age, sex, aneurysm location, aneurysm size, neck width, Hunt-Hess grade, and modified Fisher grade. Perioperative procedure-related complications and clinical and angiographic follow-up outcomes were compared. Univariate and multivariate analyses were performed to determine the associations between procedure-related complications and potential risk factors.

Results
A total of 142 patients who underwent LVIS stent-assisted coiling and 93 patients who underwent laser-cut stent-assisted coiling were enrolled after 1:2 propensity score matching. The angiographic follow-up outcomes showed that the LVIS stent group had a slightly higher complete occlusion rate and lower recurrence rate than the laser-cut stent group (92.7% vs 80.6%; 3.7% vs 9.7%, P = 0.078). The clinical outcomes at discharge and follow-up between the two groups demonstrated no significant differences (P = 0.495 and P = 0.875, respectively). The rates of intraprocedural thrombosis, postprocedural thrombosis, postoperative early rebleeding, and procedure-related death were 0.7% (1/142), 1.4% (2/142), 2.8% (4/142), and 2.1% (3/142) in the LVIS stent group, respectively, and 4.3% (4/93), 2.2% (2/93), 1.1% (1/93), and 3.2% (3/93) in the laser-cut stent group, respectively (P = 0.082, 0.649, 0.651, and 0.683). Nevertheless, the rates of overall procedure-related complications and intraprocedural rupture in the LVIS stent group were significantly lower than those in the laser-cut stent group (5.6% vs 14.0%, P = 0.028; 0.7% vs 6.5%, P = 0.016). Multivariate analysis showed that laser-cut stent-assisted coiling was an independent predictor for overall procedure-related complications (OR = 2.727, P = 0.037); a history of diabetes (OR = 7.275, P = 0.027) and other cerebrovascular diseases (OR = 8.083, P = 0.022) were independent predictors for ischemic complications, whereas none of the factors were predictors for hemorrhagic complications.

Conclusions
Compared with laser-cut stent-assisted coiling, LVIS stent-assisted coiling for the treatment of acutely ruptured wide-necked intracranial aneurysms could reduce the rates of overall procedure-related complications and intraprocedural rupture.
Sentencing offenders with mental disorders, developmental disorders or neurological impairments: what does the new Sentencing Council Guideline mean for psychiatrists?
Authors: Taylor Pamela J. , Eastman Nigel , Latham Richard , Holloway Josanne 
Published: March 2021

SummaryThe new Sentencing Council Guideline on sentencing offenders with mental disorders, effective from 1 October 2020, is essential reading for all psychiatrists who give evidence in the criminal courts, revealing something of required judicial thinking, our common ground on public safety concerns but differences in focus on culpability and punishment.
New trends in quantum machine learning
(a)
Authors: Buffoni Lorenzo , Caruso Filippo 
Published: March 2021

Abstract
Here we will give a perspective on new possible interplays between machine learning and quantum physics, including also practical cases and applications. We will explore the ways in which machine learning could benefit from new quantum technologies and algorithms to find new ways to speed up their computations by breakthroughs in physical hardware, as well as to improve existing models or devise new learning schemes in the quantum domain. Moreover, there are lots of experiments in quantum physics that do generate incredible amounts of data and machine learning would be a great tool to analyze those and make predictions, or even control the experiment itself. On top of that, data visualization techniques and other schemes borrowed from machine learning can be of great use to theoreticians to have better intuition on the structure of complex manifolds or to make predictions on theoretical models. This new research field, named as quantum machine learning, is very rapidly growing since it is expected to provide huge advantages over its classical counterpart and deeper investigations are timely needed since they can be already tested on the already commercially available quantum machines.
A 4 Gene-based Immune Signature Predicts Dedifferentiation and Immune Exhaustion in Thyroid Cancer
Authors: Li Cui-Wei , Shi Xiao , Ma Ben , Wang Yu-Long , Lu Zhong-Wu , Liao Tian , Wang Yu , Ji Qing-Hai , Wei Wen-Jun 
Published: March 2021

Abstract

Context
The role of immune-related genes (IRGs) in thyroid cancer dedifferentiation and accompanying immune exhaustion remains largely unexplored.

Objective
To construct a significant IRG-based signature indicative of dedifferentiation and immune exhaustion in thyroid cancer.

Design and Settings
One exploratory cohort and 2 validation cohorts were used to identify stably dysregulated IRGs in dedifferentiated thyroid cancer (DDTC) and to obtain independent risk factors for dedifferentiation. The IRGs formed a gene signature, whose predictive value was tested by the receiver operating characteristic curve. Correlations between the signature and differentiation-related genes, immune checkpoints, and prognosis were analyzed. Gene set enrichment analyses were performed to identify related signaling pathways.

Results
Four IRGs (PRKCQ, PLAUR, PSMD2, and BMP7) were found to be repeatedly dysregulated in DDTC, and they formed an IRG-based signature with a satisfactory predictive value for thyroid cancer dedifferentiation. Correlation analyses revealed that immune checkpoints were closely related to the 4 IRGs and the IRG-based signature, which was significantly associated with the histological subtype (P = 0.026), lymph node metastasis (P = 0.001), and BRAFV600E mutation (P &lt; 0.001). The downregulated expression of PRKCQ shortened the disease-free survival for patients with thyroid cancer. Furthermore, we identified several signaling pathways inherently associated with the IRG-based signature.

Conclusions
This study suggests that IRGs participate in the dedifferentiation and immune exhaustion process of thyroid cancer and are potential biomarkers for DDTC.
Biophysical and biochemical properties of Deup1 self-assemblies: a potential driver for deuterosome formation during multiciliogenesis
Authors: Yamamoto Shohei , Yabuki Ryoichi , Kitagawa Daiju 
Published: March 2021

ABSTRACT
The deuterosome is a non-membranous organelle involved in large-scale centriole amplification during multiciliogenesis. Deuterosomes are specifically assembled during the process of multiciliogenesis. However, the molecular mechanisms underlying deuterosome formation are poorly understood. In this study, we investigated the molecular properties of deuterosome protein 1 (Deup1), an essential protein involved in deuterosome assembly. We found that Deup1 has the ability to self-assemble into macromolecular condensates both in vitro and in cells. The Deup1-containing structures formed in multiciliogenesis and the Deup1 condensates self-assembled in vitro showed low turnover of Deup1, suggesting that Deup1 forms highly stable structures. Our biochemical analyses revealed that an increase of the concentration of Deup1 and a crowded molecular environment both facilitate Deup1 self-assembly. The self-assembly of Deup1 relies on its N-terminal region, which contains multiple coiled coil domains. Using an optogenetic approach, we demonstrated that self-assembly and the C-terminal half of Deup1 were sufficient to spatially compartmentalize centrosomal protein 152 (Cep152) and polo like kinase 4 (Plk4), master components for centriole biogenesis, in the cytoplasm. Collectively, the present data suggest that Deup1 forms the structural core of the deuterosome through self-assembly into stable macromolecular condensates.
This article has an associated First Person interview with the first author of the paper.
A mechanogenetic role for the actomyosin complex in branching morphogenesis of epithelial organs
Authors: Kim Jin Man , Jo YoungJu , Jung Ju Won , Park Kyungpyo 
Published: March 2021

ABSTRACT
The actomyosin complex plays crucial roles in various life processes by balancing the forces generated by cellular components. In addition to its physical function, the actomyosin complex participates in mechanotransduction. However, the exact role of actomyosin contractility in force transmission and the related transcriptional changes during morphogenesis are not fully understood. Here, we report a mechanogenetic role of the actomyosin complex in branching morphogenesis using an organotypic culture system of mouse embryonic submandibular glands. We dissected the physical factors arranged by characteristic actin structures in developing epithelial buds and identified the spatial distribution of forces that is essential for buckling mechanism to promote the branching process. Moreover, the crucial genes required for the distribution of epithelial progenitor cells were regulated by YAP and TAZ through a mechanotransduction process in epithelial organs. These findings are important for our understanding of the physical processes involved in the development of epithelial organs and provide a theoretical background for developing new approaches for organ regeneration.
Defining the signalling determinants of a posterior ventral spinal cord identity in human neuromesodermal progenitor derivatives
Authors: Wind Matthew , Gogolou Antigoni , Manipur Ichcha , Granata Ilaria , Butler Larissa , Andrews Peter W. , Barbaric Ivana , Ning Ke , Guarracino Mario R. , Placzek Marysia , Tsakiridis Anestis 
Published: March 2021

ABSTRACT
The anteroposterior axial identity of motor neurons (MNs) determines their functionality and vulnerability to neurodegeneration. Thus, it is a crucial parameter in the design of strategies aiming to produce MNs from human pluripotent stem cells (hPSCs) for regenerative medicine/disease modelling applications. However, the in vitro generation of posterior MNs corresponding to the thoracic/lumbosacral spinal cord has been challenging. Although the induction of cells resembling neuromesodermal progenitors (NMPs), the bona fide precursors of the spinal cord, offers a promising solution, the progressive specification of posterior MNs from these cells is not well defined. Here, we determine the signals guiding the transition of human NMP-like cells toward thoracic ventral spinal cord neurectoderm. We show that combined WNT-FGF activities drive a posterior dorsal pre-/early neural state, whereas suppression of TGFβ-BMP signalling pathways promotes a ventral identity and neural commitment. Based on these results, we define an optimised protocol for the generation of thoracic MNs that can efficiently integrate within the neural tube of chick embryos. We expect that our findings will facilitate the comparison of hPSC-derived spinal cord cells of distinct axial identities.
Determination of field capacity in the Chibunga and Guano rivers micro-basins
Authors: Mendoza Benito , Fiallos Manuel , Iturralde Sandra , Santillán Patricio , Guananga Nelly , Bejar Jaime , Lowy Daniel A. , Vágó Imre , Sándor Zsolt 
Published: March 2021

Background: The micro-basins of the Chibunga and Guano rivers are located within the sub-basin of the Chambo River, which starts at the thaw of the Chimborazo, crosses the cities of Guano and Riobamba, and ends in the Chambo River. These rivers are considered fluvial hydrological forces and geological limits of the aquifer, located in this sub-basin. For this reason, our investigation addressed the field capacity in the micro-basins of Chibunga and Guano rivers, to determine the maximum retention potential, i.e., the saturation of water in the soil. Methods: We investigated the change of precipitation to runoff through the correlations between the characteristics of the soil and its vegetation. We applied the Curve Number (CN) method introduced by the United States Soil Conservation Service (USSCS); this represents an empirical model, which relates the vegetation cover to the geological and topographic conditions of the soil. Along with the geographic information system, the model allows to represent the variation of runoffs for each micro-basin, according to the different land use categories, over the time frame from 2010 to 2014. Results: We found that the maximum retention potential is directly affected by CN values, representing the runoff potential. Highest values of 100 belong to the wetlands, urban area, snow, and water, as rain is converted directly into runoff, being impervious areas. The Guano river micro-basin possesses clay soil with CN of 78, the soil texture for eucalyptus forest is clay loam, and its CN value, 46, is the lowest of the data set. Knowledge of field capacity allows to properly evaluate the storage capacity of soil and water conservation. Conclusions: Results of this work will be useful in the quantification of the water balance, to determine the water supply and demand.
Proposal for phase-sensitive heterodyne detection in large-scale passive resonant gyroscopes
Authors: Li Zongyang , Liu Kui , Liu Jing , Lu Zehuang , Zhang Jie 
Published: March 2021

Large-scale passive resonant gyroscopes (PRGs) have been utilized in the measurement of Earth rotation. We report on a scheme of phase-sensitive heterodyne detection in large-scale PRGs. By injecting three separated beams into different longitudinal modes of the ring cavity and self-demodulating the detected signals, the backscattering disturbance and the cavity length fluctuation effect both can be isolated. With the implementation of this new scheme, we can obtain the Earth rotation signal with a Sagnac frequency that is twice of that of the traditional scheme, which enhance the equivalent scale factor of the laser gyroscopes. On the other hand, the quantum noise limit of the instrument can also be further suppressed due to the improvement of the signal-to-noise ratio. With this new scheme, the theoretical rotational sensitivity of a 3 m × 3 m large scale PRG can be as low as

10

−
12

rad

/

s

/

Hz

. With this rotational sensitivity, the measurement of the length of day or the test of the general relativity can be realized.
The potential for histone deacetylase (HDAC) inhibitors as cestocidal drugs
Authors: Vaca Hugo Rolando , Celentano Ana María , Toscanini María Agustina , Heimburg Tino , Ghazy Ehab , Zeyen Patrik , Hauser Alexander-Thomas , Oliveira Guilherme , Elissondo María Celina , Jung Manfred , Sippl Wolfgang , Camicia Federico , Rosenzvit Mara Cecilia 
Published: March 2021

Background
Echinococcosis and cysticercosis are neglected tropical diseases caused by cestode parasites (family Taeniidae). Not only there is a small number of approved anthelmintics for the treatment of these cestodiases, but also some of them are not highly effective against larval stages, such that identifying novel drug targets and their associated compounds is critical. Histone deacetylase (HDAC) enzymes are validated drug targets in cancers and other diseases, and have been gaining relevance for developing new potential anti-parasitic treatments in the last years. Here, we present the anthelmintic profile for a panel of recently developed HDAC inhibitors against the model cestode Mesocestoides vogae (syn. M. corti).

Methodology/Principal findings
Phenotypic screening was performed on M. vogae by motility measurements and optical microscopic observations. Some HDAC inhibitors showed potent anthelmintic activities; three of them -entinostat, TH65, and TH92- had pronounced anthelmintic effects, reducing parasite viability by ~100% at concentrations of ≤ 20 μM. These compounds were selected for further characterization and showed anthelmintic effects in the micromolar range and in a time- and dose-dependent manner. Moreover, these compounds induced major alterations on the morphology and ultrastructural features of M. vogae. The potencies of these compounds were higher than albendazole and the anthelmintic effects were irreversible. Additionally, we evaluated pairwise drug combinations of these HDAC inhibitors and albendazole. The results suggested a positive interaction in the anthelmintic effect for individual pairs of compounds. Due to the maximum dose approved for entinostat, adjustments in the dose regime and/or combinations with currently-used anthelmintic drugs are needed, and the selectivity of TH65 and TH92 towards parasite targets should be assessed.

Conclusion, significance
The results presented here suggest that HDAC inhibitors represent novel and potent drug candidates against cestodes and pave the way to understanding the roles of HDACs in these parasites.