bluuebunny/crossref_metadata_2025_split huggingface dataset:
Computationally designed GPCR quaternary structures bias signaling pathway activation
Authors: Paradis J. S. , Feng X. , Murat B. , Jefferson R. , Szpakowska M. , Hogue M. , Bergkamp N. D. , Heydenreich F.M. , Smit M. J. , Chevigne A. , Bouvier M. , Barth P. 
Published: September 2021

AbstractCommunication across membranes controls critical cellular processes and is achieved by receptors translating extracellular signals into selective cytoplasmic responses. While receptor tertiary structures can now be readily characterized, receptor associations into quaternary structures are very challenging to study and their implications in signal transduction remain poorly understood. Here, we report a computational approach for predicting membrane receptor self-associations, and designing receptor oligomers with various quaternary structures and signaling properties. Using this approach, we designed chemokine receptor CXCR4 dimers with reprogrammed stabilities, conformations, and abilities to activate distinct intracellular signaling proteins. In agreement with our predictions, the designed CXCR4s dimerized through distinct conformations and displayed different quaternary structural changes upon activation. Consistent with the active state models, all engineered CXCR4 oligomers activated the G protein Gi, but only a few specific dimer structures also recruited β-arrestins. Overall, we demonstrate that quaternary structures represent an important unforeseen mechanism of receptor biased signaling and reveal the existence of a conformational switch at the dimer interface of several G protein-coupled receptors including CXCR4, mu-Opioid and type-2 Vasopressin receptors that selectively control the activation of G proteins vs β-arrestin-mediated pathways. The approach should prove useful for predicting and designing receptor associations to uncover and reprogram selective cellular signaling functions.
Transcriptome and miRNAome reveal components regulating primary thickening of bamboo shoots
Authors: Li Ying , Zhang Deqiang , Lou Yongfeng , Xinmin An , Gao Zhimin 
Published: September 2021

AbstractPrimary thickening determines bamboo yield and wood property. However, little is known about the regulatory networks involved in this process. The present study identified a total of 58,652 genes and 521 miRNAs via transcriptome and small RNA sequencing using the underground thickening shoot samples of wild type (WT) Moso bamboo (Phyllostachys edulis) and a thick wall (TW) variant (P. edulis cv. Pachyloen) at five developmental stages (WTS1/TWS1-WTS5/TWS5). A total of 11,636 (54.05%) differentially expressed genes (DEGs) and 515 (98.85%) differentially expressed miRNAs (DEmiRs) were identified from the WT, TW, and WTTW groups. The first two groups were composed of four pairwise combinations each between two successive stages (WTS2/TWS2_vs_WTS1/TWS1, WTS3/TWS3_vs_WTS2/TWS2, WTS4/TWS4_vs_WTS3/TWS3 and WTS5/TWS5_vs_WTS4/TWS4), and the WTTW group was composed of five between two relative stages (TWS1–5_vs_WTS1–5). Additionally, among the phytohormones, zeatin (ZT) showed more remarkable changes in concentrations than indole-3-acetic acid (IAA), gibberellic acid (GA3), and abscisic acid (ABA) throughout the five stages in the WT and the TW groups. Moreover, 118 sites were identified for 590 miRNA-mRNA pairs via degradome sequencing. The dual-luciferase reporter assay confirmed that 14 miRNAs bound to 12 targets. Fluorescence in situ hybridization (FISH) localized miR166 and miR160 in the shoot apical meristem (SAM) and the procambium of Moso bamboo shoots at the S1 stage. Thus, primary thickening is a complex process regulated by miRNA-gene-phytohormone networks, and the miRNAome and transcriptome dynamics regulate phenotypic plasticity. These findings provide insights into the molecular mechanisms underlying wood formation and properties and propose targets for bamboo breeding.
Structural basis of substrate recognition and translocation by human ABCD1
Authors: Chen Zhi-Peng , Xu Da , Wang Liang , Zhou Cong-Zhao , Hou Wen-Tao , Chen Yuxing 
Published: September 2021

AbstractHuman ATP-binding cassette (ABC) transporter ABCD1 transports CoA esters of saturated/monounsaturated very long chain fatty acid from cytosol to the peroxisome for β-oxidation. Dysfunction of human ABCD1 usually causes the severe progressive genetic disorder X-linked adrenoleukodystrophy, which eventually affects the adrenal glands and/or the central nervous system. Here, we report three cryo-EM structures of human ABCD1 in various states. The apo-form ABCD1 at 3.53 Å resolution adopts an inward-facing conformation, harboring a phosphatidyl ethanolamine (PE) molecule at each lateral entry of substrate cavity. In the substrate-bound ABCD1 structure at 3.59 Å resolution, two molecules of C22:0-CoA (one of the physiological substrates of ABCD1) is symmetrically bound to the transmembrane domains (TMDs). Each C22:0-CoA adopts an unpresented L-shape configuration: the CoA portion inserts into a polar pocket at the TMD at a pose parallel to the membrane plane, whereas the acyl chain portion perpendicular to membrane plane is embedded in a hydrophobic pocket at the opposite TMD. Upon binding the two C22:0-CoA molecules, which resemble a pair of hinges crossing the two TMDs, the two nucleotide-binding domains (NBDs) of ABCD1 approach towards each other. Addition ATP to the substrate-bound ABCD1 enabled us to reveal an ATP-bound structure at 2.79 Å, which shows an outward-facing conformation with the dimerized NBDs succeeding substrate release. These three structures combined with biochemical assays exhibit a snapshot of ABCD1-mediated substrate recognition, translocation and release. These findings provide the structural insights into the transport mechanism of ABC transporters that transport amphipathic molecules with long acyl chains.
Unmanned aerial vehicles (UAV) as a tool for visual inspection of building facades in AEC+FM industry
Authors: Ruiz Ramiro Daniel Ballesteros , Lordsleem Jr. Alberto Casado , Rocha Joaquin Humberto Aquino , Irizarry Javier 
Published: September 2021

Purpose
The purpose of this paper is to report on the results of an exploratory study on the use of unmanned aerial vehicles (UAV) as a visual data collection tool in the architecture, engineering, construction and facility management industry for the inspection of pathological manifestations in building facades.

Design/methodology/approach
The methodology used a field research experimental approach, where three case studies were carried out involving buildings of medium and high elevation. The protocol of activities included image collection and processing stages, as well as detailed analysis of the collected visual data for the identification of pathological manifestations in building facades.

Findings
The findings emphasize the technical feasibility and efficacy of inspections with UAV, showing that among the visual assets produced, digital photographs collected with the aircraft were more effective for the detection of pathologies when compared to the three-dimensional models and orthomosaics generated by digital photogrammetry software.

Originality/value
The research has formulated the protocol for the inspection of facades using UAV and the comparative analysis of visual assets that can be generated for inspection purposes.
Fourth kind Chebyshev Wavelet Method for the solution of multi-term variable order fractional differential equations
Authors: Turan Dincel Arzu , Tural Polat Sadiye Nergis 
Published: September 2021

PurposeMulti-term variable-order fractional differential equations (VO-FDEs) are powerful tools in accurate modeling of transient-regime real-life problems such as diffusion phenomena and nonlinear viscoelasticity. In this paper the Chebyshev polynomials of the fourth kind is employed to obtain a numerical solution for those multi-term VO-FDEs.Design/methodology/approachTo this end, operational matrices for the approximation of the VO-FDEs are obtained using the Fourth kind Chebyshev Wavelets (FKCW). Thus, the VO-FDE is condensed into an algebraic equation system. The solution of the system of those equations yields a coefficient vector, the coefficient vector in turn yields the approximate solution.FindingsSeveral examples that we present at the end of the paper emphasize the efficacy and preciseness of the proposed method.Originality/valueThe value of the paper stems from the exploitation of FKCWs for the numerical solution of multi-term VO-FDEs. The method produces accurate results even for relatively small collocation points. What is more, FKCW method provides a compact mapping between multi-term VO-FDEs and a system of algebraic equations given in vector-matrix form.
An investigation into challenges and opportunities in the Australian construction and demolition waste management system
Authors: Shooshtarian Salman , Caldera Savindi , Maqsood Tayyab , Ryley Tim , Khalfan Malik 
Published: September 2021

PurposeThe literature shows that the current Australian waste management framework does not meet industry and government expectations. This study, therefore, seeks the key construction and demolition (C&D) stakeholders' insights on various issues identified. It aims to understand the main barriers to effective C&D waste management, examining the role of the federal government and exploring perceptions around waste regulations, policies and schemes.Design/methodology/approachAn online survey was conducted in 2019 to capture stakeholder perceptions. One hundred and thirty-two participants from various industries and government agencies representing Australian jurisdictions took part in the survey. Quantitative and qualitative data were collected.FindingsThe results show that the main barriers are “overregulation, tough acceptance criteria and increased testing requirements”, “lack of local market” and “culture, poor education and low acceptance”. The main areas of improvement include “providing a guideline that determines the accepted level of contamination for reusing C&D waste”, “preparation of guidelines on requirements of using recycled C&D materials in different industries” and “setting a target for reduction, reusing and recycling C&D waste”.Research limitations/implicationsSome research findings may not be generalisable beyond Australia, but there are interesting insights for an international audience. The results inform policy development within the Australian states and territories context, to support the design and implementation of a circular economy model in the construction industry. The findings are evidence for a broader discussion to solve prevailing issues in C&D waste management, notably in the context of construction materials' end of life management.Practical implicationsThe study highlights that policy development needs to be further expanded to include new/current waste management schemes including manufacturer's shared responsibility of waste generation, subsidies for C&D waste recycled materials and the proximity principle.Originality/valueThis paper provides a clear insight into C&D waste management stakeholders' perceptions towards the current waste management system in Australia.
Internships before and during COVID-19: experiences and perceptions of undergraduate interns and supervisors
Authors: Teng Cecilia Woon Chien , Lim Raymond Boon Tar , Chow Dana Wai Shin , Narayanasamy Suganthi , Liow Chee Hsiang , Lee Jeannette Jen-Mai 
Published: September 2021

PurposeThe COVID-19 pandemic has brought about a contingent shift to remote working and learning worldwide. However, little is known regarding the impact of this shift on internships. Moreover, much of the available literature studies on internships are focused largely on perceptions by students, less so by supervisors. This paper describes the impact of COVID-19 on public health (PH) internships and examines interns' and supervisors' perspectives on their experiences in internships before and during the pandemic.Design/methodology/approachA cross-sectional study design was conducted on two cohorts of undergraduate students and their supervisors in Singapore. Participants were surveyed using questionnaires with both close-ended and open-ended questions about various aspects of the internship experience. Data were triangulated from these surveys and module evaluation reports, and analyzed quantitatively and qualitatively.FindingsCOVID-19 disrupted internships significantly, with a reduction in the number of placements offered and necessary changes to the internship scope. Overall, the internship experience has been positive. Supervisors and e-interns reported high levels of satisfaction and documented learning gains such as the development of technical skills and soft skills unique to remote work.Originality/valueThe study findings fill current gaps in the literature on supervisor perceptions and internship experiences during COVID-19. Recommendations are proposed to optimize e-internships, a potentially authentic workplace in the post-COVID era.
Characteristics of generalized Fourier’s heat flux and homogeneous-heterogeneous reactions in 3D flow of non-Newtonian cross fluid
Authors: Ali Mehboob , Sultan F. , Khan Waqar Azeem , Shahzad M. , Arif Hina , Irfan M. 
Published: September 2021

Purpose
The purpose of this paper is to investigate the heat transportation rate by using Cattaneo–Christov heat flux model. Furthermore, homogeneous-heterogeneous reaction is also deliberated in the modeling of concentration expression.

Design/methodology/approach
The nonlinear PDEs are reduced to ODEs via implementation of applicable transformations. Numerical scheme bvp4c is used to obtain convergent solutions.

Findings
The main findings are to characterize the generalized Fourier’s heat flux and homogeneous-heterogeneous reactions in 3D flow of non-Newtonian cross fluid.

Originality/value
It is to certify that this paper is neither published earlier nor submitted elsewhere.
Poverty prevalence and negative spillovers in Sub-Saharan Africa: a focus on extreme and multidimensional poverty in the region
Authors: Olaoye Olumide , Ibukun Cleopatra Oluseye , Razzak Mustafa , Mose Naftaly 
Published: September 2021

PurposeThe paper analyses the prevalence of extreme and multidimensional poverty in line with the sustainable development agenda. In addition, the paper examines the drivers of extreme poverty while accounting for the potential spillover effect of poverty in the region.Design/methodology/approachThe study adopts the pooled OLS with Discroll-Kraay robust standard errors to control for cross-sectional dependence. In addition, given the strong potential for endogeneity of poverty index, the authors also employ the generalized method of moments (GMM), which accounts for simultaneity and endogeneity problems, and the spatial error and lag models to control for all forms of spatial and temporal dependence since the factors that affect poverty disperse across borders.FindingsThe study finds that in addition to the traditional drivers of poverty (unemployment, low per capita GDP growth and public debt), poverty in Sub-Saharan Africa is a symptom of a deeper structural problem (lack of access to water and sanitation, high level of corruption and low level of financial development, and frequent economic busts). Likewise, the results from the spatial econometric specification show, consistently across all the specifications, that there is a substantial spillover effect of poverty across the region.Originality/valueThe main novelty of the paper is that the authors investigate the “economic shrinkage hypothesis,” and examined the potential negative spillover effect of poverty in the region.
Conspicuous consumption: impact of narcissism and need for uniqueness on self-brand and communal-brand connection with public vs private use brands
Authors: Fazli-Salehi Reza , Torres Ivonne M. , Madadi Rozbeh , Zúñiga Miguel Ángel 
Published: September 2021

Purpose
The purpose of this study was to explore the impact of self-related traits on consumer self-brand connection (SBC) and communal-brand connection (CBC) in public vs private consumption. Marketing practitioners will benefit by understanding the consumer traits that can be triggered and focused on in advertising campaigns. Moreover, it is important to know which traits have a significant impact on each product category section (i.e. public vs private use).

Design/methodology/approach
An experiment used a between-subjects design with two randomized blocks of publicly used brands and privately used brands. Within each block, the relationship between independent variables (narcissism and need for uniqueness [NFU]) and dependent variables (SBC, CBC and purchase intention) were analyzed using SmartPLS.

Findings
The results showed that narcissism has a significant positive impact on consumers’ SBC for publicly consumed products, no effect was found for CBC. NFU shows a significant positive impact on SBC and CBC for both categories. The results also showed a positive impact for SBC on purchase intention, no effect was found for CBC.

Research limitations/implications
Future research can examine brand names that people favor the most and test whether individuals’ narcissism and NFU is influential on their SBC and CBC with the brands, regardless of the exposure to the visual cues provided in this paper. Moreover, there are more behavioral outcomes that need examination. For example, it would be fruitful to see whether attitude toward the ad, or brand attitude are affected by consumers’ narcissism and NFU.

Practical implications
The results contribute to the effectiveness of advertising in different industries. For instance, brand managers and marketing practitioners can benefit by understanding which product types are more attractive to consumers based on their tendency toward uniqueness. Moreover, narcissism is another common trait that can be used to target consumers. Thus, certain product types may be more attractive to consumers based on their narcissism tendencies.

Originality/value
Despite the popularity of SBC and CBC research, there is limited knowledge about the consumer traits which can stimulate and enhance these concepts. As such, an important question that needs to be addressed is: “What consumer personality traits lead to higher SBC and CBC?” When it comes to self-concept, a limited number of studies exist that explore the actual types of personality or self-concept that generate a desired connection between the “self” and brands.
Leveraging blockchain technology for circularity in agricultural supply chains: evidence from a fast-growing economy
Authors: Sharma Rohit , Samad Taab Ahmad , Chiappetta Jabbour Charbel Jose , de Queiroz Mauricio Juca 
Published: September 2021

PurposeThe authors originally explore the factors for blockchain technology (BCT) adoption in agricultural supply chains (ASCs) to enhance circularity and understand the dependencies, hierarchical structure and causalities between these factors.Design/methodology/approachBased on an extant literature review and expert opinion, the present study identified ten enablers for adopting BCT to leverage the circular economy (CE) practices in the ASCs. Then, using an integrated interpretive structural modeling and decision-making trial and evaluation laboratory (ISM-DEMATEL) approach, hierarchical and cause–effect relationships are established.FindingsIt was observed that traceability is the most prominent enabler from the CE perspective in ASCs. However, traceability, being a net effect enabler, will be realized through the achievement of other cause enablers, such as seamless connectivity and information flow and decentralized and distributed ledger technology. The authors also propose a 12 Rs framework for enhancing circularity in ASC operations.Research limitations/implicationsThe paper identifies enablers to BCT adoption that will enhance circularity in ASC operations. The ISM hierarchical model is based on the driving and dependence powers of the enablers, and DEMATEL aids in identifying causal relationships among the enablers.Practical implicationsThe study's findings and proposed 12 Rs framework may help the practitioners and policymakers devise effective BCT implementation strategies in ASCs, thereby empowering sustainability and circularity.Originality/valueThis study enriches the literature by identifying and modeling enablers for BCT adoption in ASCs. The study also proposes a new 12 Rs framework to help enhance ASC circularity.
Volatility discovery in cryptocurrency markets
Authors: Dimpfl Thomas , Elshiaty Dalia 
Published: September 2021

PurposeCryptocurrency markets are notoriously noisy, but not all markets might behave in the exact same way. Therefore, the aim of this paper is to investigate which one of the cryptocurrency markets contributes the most to the common volatility component inherent in the market.Design/methodology/approachThe paper extracts each of the cryptocurrency's markets' latent volatility using a stochastic volatility model and, subsequently, models their dynamics in a fractionally cointegrated vector autoregressive model. The authors use the refinement of Lien and Shrestha (2009, J. Futures Mark) to come up with unique Hasbrouck (1995, J. Finance) information shares.FindingsThe authors’ findings indicate that Bitfinex is the leading market for Bitcoin and Ripple, while Bitstamp dominates for Ethereum and Litecoin. Based on the dominant market for each cryptocurrency, the authors find that the volatility of Bitcoin explains most of the volatility among the different cryptocurrencies.Research limitations/implicationsThe authors’ findings are limited by the availability of the cryptocurrency data. Apart from Bitcoin, the data series for the other cryptocurrencies are not long enough to ensure the precision of the authors’ estimates.Originality/valueTo date, only price discovery in cryptocurrencies has been studied and identified. This paper extends the current literature into the realm of volatility discovery. In addition, the authors propose a discrete version for the evolution of a markets fundamental volatility, extending the work of Diaset al.(2018).
Media use, political trust and attitude toward direct democracy: empirical evidence from Taiwan
Authors: Chang Wen-Chun 
Published: September 2021

PurposeThis study examines the roles of the Internet and other types of media use in explaining the support for direct democracy and further investigates the mediation of political trust in the relationship between media use and the attitude toward direct democracy.Design/methodology/approachUsing data drawn from Taiwan Social Change Survey 2014 and the approach of structural equation model framework, this study identifies the indirect effects of the Internet and other types of media use on the attitude toward referendums.FindingsThe results of this study show that the frustration resulting from the process of representative politics dominated by political elites is associated with the support for direct democracy as an effective alternative to generate political influences in the formation of public policies.Originality/valueThe advances in the Internet and information technology have expanded the possible platforms of obtaining political information and enabled people to rapidly access political information at lower costs. It is expected that Internet use has altered the relationships among citizens, political parties and the government, potentially influencing citizens' political trust and their attitude toward direct democracy.