bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effects of drivers and their variations on the number of stems and aboveground carbon removals in miombo woodlands of mainland Tanzania
Authors: Manyanda Bernardol John , Nzunda Emmanuel F. , Mugasha Wilson Ancelm , Malimbwi Rogers Ernest 
Published: May 2021

Abstract
Background
Removals caused by both natural and anthropogenic drivers such as logging and fire in miombo woodlands causes substantial carbon emissions. Here we present drivers and their effects on the variations on the number of stems and aboveground carbon (AGC) removals based on an analysis of Tanzania’s national forest inventory (NFI) data extracted from the National Forest Resources Assessment and Monitoring (NAFORMA) database using allometric models that utilize stump diameter as the sole predictor.

Results
Drivers of AGC removals in miombo woodlands of mainland Tanzania in order of importance were timber, fire, shifting cultivation, charcoal, natural death, firewood collection, poles, grazing by wildlife animals, carvings, grazing by domestic animals, and mining. The average number of stems and AGC removals by driver ranged from 0.006 to 16.587 stems ha−1 year−1 and 0.0–1.273 tCha−1 year−1 respectively. Furthermore, charcoal, shifting cultivation and fuelwood caused higher tree removals as opposed to timber, natural death and fire that accounted for higher AGC removals.

Conclusions
Drivers caused substantial effects on the number of stems and carbon removals. Increased mitigation efforts in addressing removals by timber, fires, shifting cultivation, charcoal and natural death would be effective in mitigating degradation in miombo woodlands of Tanzania. Additionally, site-specific studies need to be conducted to bring information that would be used for managing woodlands at local levels. This kind of study need to be conducted in other vegetation types like montane and Mangrove forest at national scale in Tanzania.
The natural history of Canavan disease: 23 new cases and comparison with patients from literature
Authors: Bley Annette , Denecke Jonas , Kohlschütter Alfried , Schön Gerhard , Hischke Sandra , Guder Philipp , Bierhals Tatjana , Lau Heather , Hempel Maja , Eichler Florian S. 
Published: May 2021

Abstract
Background
Canavan disease (CD, MIM # 271900) is a rare and devastating leukodystrophy of early childhood. To identify clinical features that could serve as endpoints for treatment trials, the clinical course of CD was studied retrospectively and prospectively in 23 CD patients. Results were compared with data of CD patients reported in three prior large series. Kaplan Meier survival analysis including log rank test was performed for pooled data of 82 CD patients (study cohort and literature patients).

Results
Onset of symptoms was between 0 and 6 months. Psychomotor development of patients was limited to abilities that are usually gained within the first year of life. Macrocephaly became apparent between 4 and 18 months of age. Seizure frequency was highest towards the end of the first decade. Ethnic background was more diverse than in studies previously reported. A CD severity score with assessment of 11 symptoms and abilities was developed.

Conclusions
Early hallmarks of CD are severe psychomotor disability and macrocephaly that develop within the first 18 months of life. While rare in the first year of life, seizures increase in frequency over time in most patients. CD occurs more frequently outside Ashkenazi Jewish communities than previously reported. Concordance of phenotypes between siblings but not patients with identical ASPA mutations suggest the influence of yet unknown modifiers. A CD severity score may allow for assessment of CD disease severity both retrospectively and prospectively.
Identification of two novel bullous pemphigoid- associated alleles, HLA-DQA1*05:05 and -DRB1*07:01, in Germans
Authors:   German AIBD Study Group, Schwarm Christian , Gola Damian , Holtsche Maike M. , Dieterich Anabelle , Bhandari Anita , Freitag Miriam , Nürnberg Peter , Toliat Mohammad , Lieb Wolfgang , Wittig Michael , Franke André , Worm Margitta , Sticherling Michael , Ehrchen Jan , Günther Claudia , Gläser Regine , Peitsch Wiebke K. , Sárdy Miklós , Eming Rüdiger , Hertl Michael , Benoit Sandrine , Goebeler Matthias , Pföhler Claudia , Kunz Manfred , Kreuter Alexander , van Beek Nina , Erdmann Jeanette , Busch Hauke , Zillikens Detlef , Sadik Christian D. , Hirose Misa , König Inke R. , Schmidt Enno , Ibrahim Saleh M. 
Published: May 2021

AbstractBullous pemphigoid (BP) is the most common autoimmune skin blistering disease characterized by autoimmunity against the hemidesmosomal proteins BP180, type XVII collagen, and BP230. To elucidate the genetic basis of susceptibility to BP, we performed the first genome-wide association study (GWAS) in Germans. This GWAS was combined with HLA locus targeted sequencing in an additional independent BP cohort. The strongest association with BP in Germans tested in this study was observed in the two HLA loci, HLA-DQA1*05:05 and HLA-DRB1*07:01. Further studies with increased sample sizes and complex studies integrating multiple pathogenic drivers will be conducted.
Bowel incarceration within the vaginal tunic in a three-and-half-year-old bilaterally cryptorchid Lhasa Apso
Authors: Kodie Dorcas Oyueley , Oyetayo Noah Segun , Awoyemi Oladotun Solomon , Oguntoye Cecelia Omowunmi , Eyarefe Oghenemega David 
Published: May 2021

Abstract
Background
Cryptorchidism in dogs is of clinical concern due to its association with development of Sertoli cell tumours, seminomas and spermatic cord torsion. A patent inguinal ring has been found as a risk factor for peritoneal content migration and inguinal hernias. This study reports a case of bowel migration through a patent inguinal ring in a bilaterally cryptorchid dog and incarceration within the vaginal tunic of the left testicle.

Case presentation
A three-and-a-half-year-old bilaterally cryptorchid Lhasa Apso with a history of anorexia, vomiting, stranguria and inability to defecate was diagnosed with bowel incarceration in the vaginal tunic of a retained left testicle. Surgery performed under epidural anaesthesia with acepromazine/butorphanol premedication revealed a loop of the colon entrapped in the vaginal tunic of the retained left testicle. The incarcerated bowel was thoroughly examined for viability and repositioned into the abdominal cavity. The inguinal ring was repaired and bilateral cryptorchidectomy performed.

Conclusion
Cryptorchidectomy in dogs is often considered when there is concern for neoplasm or torsion of retained testes. However, this report suggests that cryptorchidectomy should be considered also to preclude the possibility of bowel obstructive emergencies.
Effect of hydroxychloroquine on COVID-19 prevention in cancer patients undergoing treatment: study protocol for a randomized controlled trial
Authors: Rahimi H. , Allahyari A. , Ataei Azimi S. , Kamandi M. , Mozaheb Z. , Zemorshidi F. , Khadem-Rezaiyan M. , Bary A. , Seddigh-Shamsi M. , Nodeh M. Moeini 
Published: May 2021

Abstract
Objectives
In this study, we will investigate the effect of hydroxychloroquine on the prevention of novel coronavirus disease (COVID-19) in cancer patients being treated.

Trial design
This is a two-arm, parallel-group, triple-blind, phase 2–3 randomized controlled trial.

Participants
All patients over the age of 15 years from 5 types of cancer will be included in the study. Patients with acute lymphoid and myeloid leukemias in the first line treated with curative intent, patients with high-grade non-Hodgkin’s lymphoma treated with leukemia regimens, and patients with non-metastatic breast and colon cancer in the first line of treatment will enter the study.

Intervention and comparator
Patients are randomly assigned to two groups: one being given hydroxychloroquine and the other is given placebo. During 2 months of treatment, the two groups will be treated with hydroxychloroquine every other day with a single 200-mg tablet (Amin® Pharmaceutical Company, Isfahan, Iran) or placebo (identical in terms of shape, color, and smell). Patients will be monitored for COVID-19 symptoms during follow-up period.
If any COVID-19-related signs or symptoms occur, they will be examined, thoroughly, investigated with a high resolution computerize tomography (CT) scan of the lungs and nasopharyngeal swab assessed by RT-PCR for SARS-CoV-2 virus. This study will be performed in five centers affiliated to Mashhad University of Medical Sciences, Mashhad, Iran.

Main outcomes
The primary end point of this study is to investigate the incidence of COVID-19 in patients being treated for their cancer and receiving prophylactic Hydroxychloroquine.

Randomization
Randomization will be performed using random permuted blocks. By using online website (www.randomization.com), the randomization sequence will be produced by quadruple blocks. The allocation ratio in intervention and control groups is 1:1.

Blinding (masking)
Participants and caregivers do not know whether the patient is in the intervention or the control group. Those assessing the outcomes and data analyzer are also blinded to group assignment.

Sample size
The calculated total sample size is 60 patients, with 30 patients in each group.
Protective mechanical ventilation with optimal PEEP during RARP improves oxygenation and pulmonary indexes
Authors: Zhou Jianwei , Wang Chuanguang , Lv Ran , Liu Na , Huang Yan , Wang Wu , Yu Lina , Xie Junran 
Published: May 2021

Abstract
Background
This trial aimed to evaluate the effects of a protective ventilation strategy on oxygenation/pulmonary indexes in patients undergoing robot-assisted radical prostatectomy (RARP) in the steep Trendelenburg position.

Methods
In phase 1, the most optimal positive end-expiratory pressure (PEEP) was determined in 25 patients at 11 cmH2O. In phase 2, 64 patients were randomized to the traditional ventilation group with tidal volume (VT) of 9 ml/kg of predicted body weight (PBW) and the protective ventilation group with VT of 7 ml/kg of PBW with optimal PEEP and recruitment maneuvers (RMs). The primary endpoint was the intraoperative and postoperative PaO2/FiO2. The secondary endpoints were the PaCO2, SpO2, modified clinical pulmonary infection score (mCPIS), and the rate of complications in the postoperative period.

Results
Compared with controls, PaO2/FiO2 in the protective group increased after the second RM (P=0.018), and the difference remained until postoperative day 3 (P=0.043). PaCO2 showed transient accumulation in the protective group after the first RM (T2), but this phenomenon disappeared with time. SpO2 in the protective group was significantly higher during the first three postoperative days. Lung compliance was significantly improved after the second RM in the protective group (P=0.025). The mCPIS was lower in the protective group on postoperative day 3 (0.59 (1.09) vs. 1.46 (1.27), P=0.010).

Conclusion
A protective ventilation strategy with lower VT combined with optimal PEEP and RMs could improve oxygenation and reduce mCPIS in patients undergoing RARP.

Trial registration
ChiCTR ChiCTR1800015626. Registered on 12 April 2018.
Portomesenteric venous thrombosis in a postmenopausal female with testosterone implant: a case report
Authors: Campitruz Monica Zanconato , Ortiz-Figueroa Luis T. , Santiago Edgardo 
Published: May 2021

Abstract
Background
Acute portal vein thrombosis is a rare medical event usually seen in liver disease, but it can also occur due to any inherited or acquired procoagulable state that triggers venous occlusion. Hormonal therapies have been associated with an increased risk of prothrombotic states. This case report documents a portomesenteric venous thrombosis in a postmenopausal woman with testosterone implant for the treatment of hypoactive sexual desire and discusses the importance of identifying hypercoagulable risk factors before initiating hormone replacement therapy. We want to improve the awareness of an unusual medical complication associated with hormone replacement therapy and shed light on how testosterone implants could facilitate a thrombotic event related to other risk factors such as obesity and chronic hypoxic states, as well as the importance of differential diagnosis in the evaluation of postmenopausal women on testosterone replacement therapy presenting with acute abdominal pain.

Case presentation
A 55-year-old obese postmenopausal Hispanic female with medical history of chronic obstructive pulmonary disease presents with intractable abdominal pain, is found to have elevated hemoglobin and hematocrit, and an abdominopelvic computed tomography scan revealing portal and superior mesenteric vein thrombosis. Further evaluation excluded inherited and acquired thrombophilia but revealed elevated testosterone levels. The patient was treated with anticoagulation, which resulted in recanalization of the portal and superior mesenteric veins.

Conclusion
Supraphysiologic levels of testosterone caused by testosterone implants as a treatment of hypoactive sexual desire in postmenopausal women can contribute to thrombotic events in the presence of additional prothrombotic risk factors. Therefore, testosterone therapy should include a thorough risk assessment for prothrombotic states, be tailored to patients’ physiologic testosterone levels, and have close follow-up with testosterone level monitoring.
GSDMD contributes to host defence against Staphylococcus aureus skin infection by suppressing the Cxcl1–Cxcr2 axis
Authors: Liu Zhen-Zhen , Yang Yong-Jun , Zhou Feng-Hua , Ma Ke , Lin Xiao-Qi , Yan Shi-Qing , Gao Yu , Chen Wei 
Published: May 2021

AbstractGasdermin D (GSDMD), a member of the gasdermin protein family, is a caspase substrate, and its cleavage is required for pyroptosis and IL-1β secretion. To date, the role and regulatory mechanism of GSDMD during cutaneous microbial infection remain unclear. Here, we showed that GSDMD protected against Staphylococcus aureus skin infection by suppressing Cxcl1–Cxcr2 signalling. GSDMD deficiency resulted in larger abscesses, more bacterial colonization, exacerbated skin damage, and increased inflammatory cell infiltration. Although GSDMD deficiency resulted in defective IL-1β production, the critical role of IL-1β was counteracted by the fact that Caspase-1/11 deficiency also resulted in less IL-1β production but did not aggravate disease severity during S. aureus skin infection. Interestingly, GSDMD-deficient mice had increased Cxcl1 secretion accompanied by increased recruitment of neutrophils, whereas Caspase-1/11-deficient mice presented similar levels of Cxcl1 and neutrophils as wild-type mice. Moreover, the absence of GSDMD promoted Cxcl1 secretion in bone marrow-derived macrophages induced by live, dead, or different strains of S. aureus. Corresponding to higher transcription and secretion of Cxcl1, enhanced NF-κB activation was shown in vitro and in vivo in the absence of GSDMD. Importantly, inhibiting the Cxcl1–Cxcr2 axis with a Cxcr2 inhibitor or anti-Cxcl1 blocking antibody rescued host defence defects in the GSDMD-deficient mice. Hence, these results revealed an important role of GSDMD in suppressing the Cxcl1–Cxcr2 axis to facilitate pathogen control and prevent tissue damage during cutaneous S. aureus infection.
3D in vitro models of skeletal muscle: myopshere, myobundle and bioprinted muscle construct
Authors: Dessauge Frederic , Schleder Cindy , Perruchot Marie-Hélène , Rouger Karl 
Published: May 2021

AbstractTypical two-dimensional (2D) culture models of skeletal muscle-derived cells cannot fully recapitulate the organization and function of living muscle tissues, restricting their usefulness in in-depth physiological studies. The development of functional 3D culture models offers a major opportunity to mimic the living tissues and to model muscle diseases. In this respect, this new type of in vitro model significantly increases our understanding of the involvement of the different cell types present in the formation of skeletal muscle and their interactions, as well as the modalities of response of a pathological muscle to new therapies. This second point could lead to the identification of effective treatments. Here, we report the significant progresses that have been made the last years to engineer muscle tissue-like structures, providing useful tools to investigate the behavior of resident cells. Specifically, we interest in the development of myopshere- and myobundle-based systems as well as the bioprinting constructs. The electrical/mechanical stimulation protocols and the co-culture systems developed to improve tissue maturation process and functionalities are presented. The formation of these biomimetic engineered muscle tissues represents a new platform to study skeletal muscle function and spatial organization in large number of physiological and pathological contexts.
Prognostic models for knee osteoarthritis: a protocol for systematic review, critical appraisal, and meta-analysis
Authors: Zhong Jingyu , Si Liping , Zhang Guangcheng , Huo Jiayu , Xing Yue , Hu Yangfan , Zhang Huan , Yao Weiwu 
Published: May 2021

Abstract
Background
Osteoarthritis is the most common degenerative joint disease. It is associated with significant socioeconomic burden and poor quality of life, mainly due to knee osteoarthritis (KOA), and related total knee arthroplasty (TKA). Since early detection method and disease-modifying drug is lacking, the key of KOA treatment is shifting to disease prevention and progression slowing. The prognostic prediction models are called for to guide clinical decision-making. The aim of our review is to identify and characterize reported multivariable prognostic models for KOA about three clinical concerns: (1) the risk of developing KOA in the general population, (2) the risk of receiving TKA in KOA patients, and (3) the outcome of TKA in KOA patients who plan to receive TKA.

Methods
The electronic datasets (PubMed, Embase, the Cochrane Library, Web of Science, Scopus, SportDiscus, and CINAHL) and gray literature sources (OpenGrey, British Library Inside, ProQuest Dissertations & Theses Global, and BIOSIS preview) will be searched from their inception onwards. Title and abstract screening and full-text review will be accomplished by two independent reviewers. The multivariable prognostic models that concern on (1) the risk of developing KOA in the general population, (2) the risk of receiving TKA in KOA patients, and (3) the outcome of TKA in KOA patients who plan to receive TKA will be included. Data extraction instrument and critical appraisal instrument will be developed before formal assessment and will be modified during a training phase in advance. Study reporting transparency, methodological quality, and risk of bias will be assessed according to the TRIPOD statement, CHARMS checklist, and PROBAST tool, respectively. Prognostic prediction models will be summarized qualitatively. Quantitative metrics on the predictive performance of these models will be synthesized with meta-analyses if appropriate.

Discussion
Our systematic review will collate evidence from prognostic prediction models that can be used through the whole process of KOA. The review may identify models which are capable of allowing personalized preventative and therapeutic interventions to be precisely targeted at those individuals who are at the highest risk. To accomplish the prediction models to cross the translational gaps between an exploratory research method and a valued addition to precision medicine workflows, research recommendations relating to model development, validation, or impact assessment will be made.

Systematic review registration
PROSPERO CRD42020203543
The impact of gunshots on place-level business activity
Authors: Stacy Christina , Irvin-Erickson Yasemin , Tiry Emily 
Published: May 2021

AbstractObjectivesGun violence can negatively affect business activity at the place-level through a variety of mechanisms. However, estimating this effect is difficult since reported crime data are biased by factors that are also associated with business health. Despite some of its limitations, data from gunshot detection technology has been shown as a new valuable source of data on gun violence (Irvin-Erickson et al. in Appl Geogr 86: 262–273, 2017a). In this study, we use gunshot detection data to explore the spatial relationship between gunshots and business activity at the neighborhood level in Washington, DC between 2010 and 2012.MethodsIn this exploratory study, we create spatial buffers of 500 and 1000 feet around each block and sum up the total number of gunshots and business births, deaths, sales, and number of employees within these buffers each year and estimate a spatial fixed effects panel model.ResultsGunshots within 1000 feet of a block increase the number of business deaths by 4.3% within that buffer on average, and gunshots within 500 feet of a block decrease the total number of service and retail businesses, the number of employees employed by businesses within that buffer, and total sales for those businesses (although not at a statistically significant rate). Gunshots on blocks with the lowest initial levels of gunshots increase business turnover and reduce the total number of businesses present by 0.5%, and gunshots on blocks with the highest initial levels of gunshots cause an increase in the number of business deaths by 7.5%.ConclusionResults suggest that efforts to improve distressed neighborhoods should target both areas with lower and higher pre-existing levels of gunshots.
Fentanyl-related overdose during incarceration: a comprehensive review
Authors: Kaplowitz Eliana , Truong Ashley Q. , Macmadu Alexandria , Peterson Meghan , Brinkley-Rubinstein Lauren , Potter Nathan , Green Traci C. , Clarke Jennifer G. , Rich Josiah D. 
Published: May 2021

Abstract
Background
Fentanyl and related compounds have recently saturated the illicit drug supply in the United States, leading to unprecedented rates of fatal overdose. Individuals who are incarcerated are particularly vulnerable, as the burden of opioid use disorder is disproportionately higher in this population, and tolerance generally decreases during incarceration.

Methods
We conduct a systematic search for publications about fentanyl overdoses during incarceration in PubMed and PsycINFO, as well as lay press articles in Google, from January 1, 2013 through March 30th, 2021.

Results
Not a single fentanyl overdose was identified in the medical literature, but 90 overdose events, comprising of 76 fatal and 103 nonfatal fentanyl overdoses, were identified in the lay press. Among the 179 overdoses, 138 occurred in jails and 41 occurred in prisons, across the country.

Conclusions
Fentanyl-related overdoses are occurring in correctional facilities with unknown but likely increasing frequency. In addition to the need for improved detection and reporting, immediate efforts to 1) increase understanding of the risks of fentanyl and how to prevent and treat overdose among correctional staff and residents, 2) ensure widespread prompt availability of naloxone and 3) expand the availability of medications to treat opioid use disorder for people who are incarcerated will save lives.