bluuebunny/crossref_metadata_2025_split huggingface dataset:
Signal integrity analysis and Peripheral Component Interconnect Express backplane link compliance assessment
Authors: Orekhov Evgeny , Smolenskiy Anton , Popov Boris 
Published: October 2021

AbstractThis article presents research conducted on the backplane high‐speed Peripheral Component Interconnect Express version 4 (PCIe gen4) link between central processor root complex and the end‐point device in order to confirm its compliance. Signal integrity analysis was performed using a method that included channel segmentation, development of corner models for channel segments, full‐wave electromagnetic simulation utilizing the finite element method (FEM) and the creation of cascaded channel models, as well as determination of the worst‐case models for the channel. The results of this analysis were then used to modify the channel design in order to decrease insertion loss. Changes in the design included utilizing a very low loss dielectric for the backplane board, correcting the sizes of differential pairs and decreasing the via stubs. Frequency domain simulation of the modified channel design has shown a 25% decrease in insertion loss. The calculation of eye diagrams for the modified design has confirmed that the channel parameters meet the requirements for the PCIe gen4 standard.
Identification of Cytochrome P450 Isozymes Involved in Enantioselective Metabolism of Fipronil in Fish Liver: In Vitro Metabolic Kinetics and Molecular Modeling
Authors: Wu Siqi , Li Huizhen , You Jing 
Published: October 2021

Abstract
Fipronil has been frequently detected in waterways worldwide at concentrations that threaten aquatic organisms, yet the metabolic behavior of fipronil enantiomers in aquatic organisms is largely unknown, which is of significance in enantioselective toxicity evaluation. We quantitatively identified the specific cytochrome P450 (CYP) isozymes involved in metabolizing fipronil enantiomers in tilapia by combining in vitro metabolic kinetic assays and molecular docking. Inhibition studies suggested that CYP1A enzyme was the main isoform catalyzing metabolism of fipronil and that CYP3A contributed in a limited way to the metabolism in fish liver S9. Both the dissipation rate constant and the maximum metabolic velocity of R-(−)-fipronil were greater than those of S-(+)-fipronil in tilapia liver S9, suggesting that tilapia selectively metabolized R-(−)-fipronil. The CYP1A1 isozyme exhibited the highest binding capacity to R-(−)-fipronil and S-(+)-fipronil (binding energy ‒9.39 and ‒9.17 kcal/mol, respectively), followed by CYP1A2 (‒7.30 and ‒6.94 kcal/mol, respectively) and CYP3A4 (‒7.16 and ‒6.91 kcal/mol, respectively). The results of in vitro metabolic assays and molecular docking were consistent, that is, CYP1A, specifically CYP1A1, exhibited a higher metabolic capacity to fipronil than CYP3A, and fish liver S9 selectively metabolized R-(−)-fipronil. The present study provides insight into the enantioselective metabolic behavior and toxicological implications of the in vitro metabolic kinetics of fipronil in fish. Environ Toxicol Chem 2022;41:230–239. © 2021 SETAC
Abstract
Tilapia selectively metabolizes R-(-)-fipronil and CYP1A is the main isozyme involving in metabolism. CYP1A/CYP3A = cytochrome P450 1A/3A.
Preparation and characterization of Zein‐sulfated Cardamine hupingshanensis polysaccharide composite films
Authors: Zhang Zimu , Huang XiuFang , Li ShiChan , Zhang Chi , Luo Kai 
Published: October 2021

AbstractCardamine hupingshanensis polysaccharide (CHP) was modified by the sulfur trioxide–pyridine method to obtain the sulfated C. hupingshanensis polysaccharide (SCHP) with a substitution degree of 0.72. The spectral results revealed that the sulfate group was successfully introduced to CHP. In the in vitro antioxidant assay, SCHP showed the highest scavenging rate of hydroxyl radicals, ABTS, and DPPH. Different concentrations of SCHP were chosen to form a compound with Zein to prepare novel bioactive films successfully. The functional and characterization studies of the films were also conducted. The scavenging ability of the films for hydroxyl radicals, ABTS, and DPPH was improved by adding different concentrations of SCHP. Although the films showed a decrease in transparency with the addition of 4 mg/ml SCHP, there was an improvement in tensile strength compared to films without the addition of SCHP. These findings indicate that Zein‐SCHP films can be used as a functional food packaging material with antioxidant properties.
Development and validation of a predictive model for pathogenic Escherichia coli in fresh‐cut produce
Authors: Kim You Jin , Park Ju Yeon , Suh Soo Hwan , Kim Mi‐Gyeong , Kwak Hyo‐Sun , Kim Soon Han , Heo Eun Jeong 
Published: October 2021

AbstractThis study was performed to develop and validate a predictive growth model of pathogenic Escherichia coli to ensure the safety of fresh‐cut produce. Samples were inoculated with a cocktail of seven E. coli strains of five pathotypes (EHEC, Enterohemorrhagic E. coli; ETEC, Enterotoxigenic E. coli; EPEC, Enteropathogenic E. coli; EIEC, Enteroinvasive E. coli, and EAEC, Enteroaggregative E. coli) and stored at 4, 10, 12, 15, 25, 30, and 37°C. Growth of pathogenic E. coli was observed above 12°C. The primary growth model for pathogenic E. coli in fresh‐cut produce was developed based on the Baranyi model. The secondary model was developed as a function of temperature for lag phase duration (LPD) and maximum specific growth rate (μmax) based on the polynomial second‐order model. The primary and secondary models for pathogenic E. coli were fitted with a high degree of goodness of fit (R2 ≥ 0.99). The bias factor (Bf), accuracy factor (Af), and root mean square error (RMSE) were 0.995, 1.011, and 0.084, respectively. The growth model we developed can provide useful data for assessing the quantitative microbial risk of pathogenic E. coli in fresh‐cut produce intended for human consumption. In addition, it is thought to be widely available in industries that produce, process, distribute, and sell fresh‐cut produce.
Astrocytic VEGFA: An essential mediator in blood–brain‐barrier disruption in Parkinson's disease
Authors: Lan Guoyu , Wang Pan , Chan Robin Barry , Liu Zongran , Yu Zhenwei , Liu Xiaodan , Yang Ying , Zhang Jing 
Published: October 2021

AbstractThe integrity of blood–brain‐barrier (BBB) is essential for normal brain functions, synaptic remodeling, and angiogenesis. BBB disruption is a common pathology during Parkinson's disease (PD), and has been hypothesized to contribute to the progression of PD. However, the molecular mechanism of BBB disruption in PD needs further investigation. Here, A53T PD mouse and a 3‐cell type in vitro BBB model were used to study the roles of α‐synuclein (α‐syn) in BBB disruption with the key results confirmed in the brains of PD patients obtained at autopsy. The A53T PD mouse studies showed that the expression of tight junction‐related proteins decreased, along with increased vascular permeability and accumulation of oligomeric α‐syn in activated astrocytes in the brain. The in vitro BBB model studies demonstrated that treatment with oligomeric α‐syn, but not monomeric or fibrillar α‐syn, resulted in significant disruption of BBB integrity. This process involved the expression and release of vascular endothelial growth factor A (VEGFA) and nitric oxide (NO) from oligomeric α‐syn treated astrocytes. Increased levels of VEGFA and iNOS were also observed in the brain of PD patients. Blocking the VEGFA signaling pathway in the in vitro BBB model effectively protected the barrier against the harmful effects of oligomeric α‐syn. Finally, the protective effects on BBB integrity associated with inhibition of VEGFA signaling pathway was also confirmed in PD mice. Taken together, our study concluded that oligomeric α‐syn is critically involved in PD‐associated BBB disruption, in a process that is mediated by astrocyte‐derived VEGFA.
The role of the arousal system in age‐related differences in cortical functional network architecture
Authors: Guardia Tiago , Geerligs Linda , Tsvetanov Kamen A. , Ye Rong , Campbell Karen L. 
Published: October 2021

AbstractA common finding in the aging literature is that of the brain's decreased within‐ and increased between‐network functional connectivity. However, it remains unclear what is causing this shift in network organization with age. Given the essential role of the ascending arousal system (ARAS) in cortical activation and previous findings of disrupted ARAS functioning with age, it is possible that age differences in ARAS functioning contribute to disrupted cortical connectivity. We test this possibility here using resting state fMRI data from over 500 individuals across the lifespan from the Cambridge Center for Aging and Neuroscience (Cam‐CAN) population‐based cohort. Our results show that ARAS‐cortical connectivity declines with age and, consistent with our expectations, significantly mediates some age‐related differences in connectivity within and between association networks (specifically, within the default mode and between the default mode and salience networks). Additionally, connectivity between the ARAS and association networks predicted cognitive performance across several tasks over and above the effects of age and connectivity within the cortical networks themselves. These findings suggest that age differences in cortical connectivity may be driven, at least in part, by altered arousal signals from the brainstem and that ARAS–cortical connectivity relates to cognitive performance with age.
Generation and validation of a PET radiomics model that predicts survival in diffuse large B cell lymphoma treated with R‐CHOP14: A SAKK 38/07 trial post‐hoc analysis
Authors: Ceriani Luca , Milan Lisa , Cascione Luciano , Gritti Giuseppe , Dalmasso Federico , Esposito Fabiana , Pirosa Maria Cristina , Schär Sämi , Bruno Andrea , Dirnhofer Stephan , Giovanella Luca , Hayoz Stefanie , Mamot Christoph , Rambaldi Alessandro , Chauvie Stephane , Zucca Emanuele 
Published: October 2021

AbstractFunctional parameters from positron emission tomography (PET) seem promising biomarkers in various lymphoma subtypes. This study investigated the prognostic value of PET radiomics in diffuse large B‐cell lymphoma (DLBCL) patients treated with R‐CHOP given either every 14 (testing set) or 21 days (validation set). Using the PyRadiomics Python package, 107 radiomics features were extracted from baseline PET scans of 133 patients enrolled in the Swiss Group for Clinical Cancer Research 38/07 prospective clinical trial (SAKK 38/07) [ClinicalTrial.gov identifier: NCT00544219]. The international prognostic indices, the main clinical parameters and standard PET metrics, together with 52 radiomics uncorrelated features (selected using the Spearman correlation test) were included in a least absolute shrinkage and selection operator (LASSO) Cox regression to assess their impact on progression‐free (PFS), cause‐specific (CSS), and overall survival (OS). A linear combination of the resulting parameters generated a prognostic radiomics score (RS) whose area under the curve (AUC) was calculated by receiver operating characteristic analysis. The RS efficacy was validated in an independent cohort of 107 DLBCL patients. LASSO Cox regression identified four radiomics features predicting PFS in SAKK 38/07. The derived RS showed a significant capability to foresee PFS in both testing (AUC, 0.709; p < 0.001) and validation (AUC, 0.706; p < 0.001) sets. RS was significantly associated also with CSS and OS in testing (CSS: AUC, 0.721; p < 0.001; OS: AUC, 0.740; p < 0.001) and validation (CSS: AUC, 0.763; p < 0.0001; OS: AUC, 0.703; p = 0.004) sets. The RS allowed risk classification of patients with significantly different PFS, CSS, and OS in both cohorts showing better predictive accuracy respect to clinical international indices. PET‐derived radiomics may improve the prediction of outcome in DLBCL patients.
Pre‐treatment health‐related quality of life parameters have prognostic impact in patients >65 years with newly diagnosed mantle cell lymphoma: The Nordic Lymphoma Group MCL4 (LENA‐BERIT) experience
Authors: Lindberg Åsa , Eskelund Christian Winther , Albertsson‐Lindblad Alexandra , Kolstad Arne , Laurell Anna , Räty Riikka , Grønbæk Kirsten , Geisler Christian Hartmann , Jerkeman Mats 
Published: October 2021

AbstractMantle cell lymphoma (MCL) is a rare, often aggressive type of B‐cell lymphoma with poor survival and no cure. Cancer and cancer treatment has a negative impact on health‐related quality of life (HRQOL) both during active disease and in the long term, and improvement of HRQOL is a crucial objective of cancer therapy in older patients and no curative intent. Baseline HRQOL has in other lymphoma populations been shown to be predictive of outcome. Here, we explored HRQOL, and its association with survival, by the EORTC QLQ‐C30 questionnaire, before, during and after chemotherapy in a patient cohort with MCL, treated within the NLG‐MCL4 trial, designed to evaluate the addition of lenalidomide (LEN) to rituximab‐bendamustine (R‐B) as first‐line treatment. Fifty‐one patients were enrolled, median age was 71 years (range 62–84), 37 were men (73%). Pre‐treatment HRQOL was similar to scores from the reference population with healthy individuals. During treatment, HRQOL deteriorated, but reverted to the same level as the reference population after treatment. There was a correlation between physical function (p = 0.001) and role function (p = 0.006) at baseline and WHO performance status, but not with other clinical or genetic prognostic factors. None of the baseline factors were predictive for treatment related to HRQOL in this cohort. Pre‐treatment physical (p = 0.011) and role function (p = 0.032) were independent factors associated with overall survival, and physical function (p = 0.002) was also associated with progression free survival. These findings may possibly be used to design support during treatment and improve rehabilitation. Further investigations are needed for assessment of long‐term HRQOL.
MHD mixed convection of a Cu–water nanofluid flow through a channel with an open trapezoidal cavity and an elliptical obstacle
Authors: Al‐Farhany Khaled , Alomari Mohammed A. , Al‐Saadi Ahmed , Chamkha Ali , Öztop Hakan F. , Al‐Kouz Wael 
Published: October 2021

AbstractIn the current work, numerical simulations are achieved to study the properties and the characteristics of fluid flow and heat transfer of (Cu–water) nanofluid under the magnetohydrodynamic effects in a horizontal rectangular canal with an open trapezoidal enclosure and an elliptical obstacle. The cavity lower wall is grooved and represents the heat source while the obstacle represents a stationary cold wall. On the other hand, the rest of the walls are considered adiabatic. The governing equations for this investigation are formulated, nondimensionalized, and then solved by Galerkin finite element approach. The numerical findings were examined across a wide range of Richardson number (0.1 ≤ Ri ≤ 10), Reynolds number (1 ≤ Re ≤ 125), Hartmann number (0 ≤ Ha ≤ 100), and volume fraction of nanofluid (0 ≤ φ ≤ 0.05). The current study's findings demonstrate that the flow strength increases inversely as the Reynolds number rises, which pushes the isotherms down to the lower part of the trapezoidal cavity. The Nuavg rises as the Ri rise, the maximum Nuavg = 10.345 at Ri = 10, Re = 50, ϕ = 0.05, and Ha = 0; however, it reduces with increasing Hartmann number. Also, it increase by increasing ϕ, at Ri = 10, the Nuavg increased by 8.44% when the volume fraction of nanofluid increased from (ϕ = 0–0.05).
Minimization of the entropy generation in MHD flow and heat transfer of nanofluid over a vertical cylinder under the influence of thermal radiation and slip condition
Authors: Agrawal Rashmi , Kaswan Pradeep 
Published: October 2021

AbstractThis investigation essentially determines the nanofluids' heat transfer performance and flow characteristics over a vertical cylinder. The influences of thermal radiation, velocity slip, and thermal slip conditions further exert effects. The mathematical model for the transport of heat and momentum is characterized by a PDE set, which is then interpreted by embracing a fourth‐order exactness program (Bvp4c). Graphical decisions reveal various parameters' values on the velocity profile, temperature profile, entropy generation, and Bejan number. A comparison of two nanofluids obtained by adding metal nanoparticles and metal oxide nanoparticles () into the base fluid () is illustrated. Heat transmission improves when the curvature parameter, mixed convection parameter, and Eckert number are raised, but it is reduced when the thermal slip parameter increases. Entropy generation is also enriched with magnetic parameter and Brinkman number, whereas it is overcome with velocity and thermal slip parameters. Furthermore, nanoparticles give a significant enhancement in the rate of heat transfer compared to nanoparticles, and entropy generation due to heat transfer is dominant for alumina nanoparticles as compared to copper nanoparticles.
Pharmacists and opioid use disorder care during COVID‐19: Call for action
Authors: Mohammad Insaf , Berri Dena , Tutag Lehr Victoria 
Published: October 2021

AbstractOpioid use disorder (OUD) is a chronic relapsing condition characterized by problematic opioid use causing significant impairment in daily life. Medication for opioid use disorder using buprenorphine, methadone, and naltrexone with behavioral therapy reduces illicit opioid use and risk of overdose death. Despite evidence and decades of experience, barriers limit access to treatment and care for individuals with OUD. Barriers include a lack of treatment centers particularly in rural areas, regulations on buprenorphine prescribing, and stigma from the community and health care professionals. While many barriers are longstanding, the coronavirus disease 2019 (COVID‐19) pandemic‐forced isolation and associated stress has exacerbated challenges for individuals with mental health conditions such as OUD. Pharmacists are well‐positioned to bridge existing gaps in OUD care, particularly during the COVID‐19 pandemic. Roles for pharmacists include OUD risk identification and screening, referral of patients to treatment and support programs, ensuring medication access, expanding naloxone access, and advocacy initiatives. This review article identifies barriers to care for patients with OUD during the COVID‐19 pandemic and explores opportunities and resources for pharmacists to improve OUD care during the pandemic and beyond.
Hydroxyapatite‐hybridized double‐network hydrogel surface enhances differentiation of bone marrow‐derived mesenchymal stem cells to osteogenic cells
Authors: Kaibara Takuma , Wang Lei , Tsuda Masumi , Nonoyama Takayuki , Kurokawa Takayuki , Iwasaki Norimasa , Gong Jian Ping , Tanaka Shinya , Yasuda Kazunori 
Published: October 2021

AbstractRecently, we have developed a hydroxyapatite (HAp)‐hybridized double‐network (DN) hydrogel (HAp/DN gel), which can robustly bond to the bone tissue in the living body. The purpose of this study is to clarify whether the HAp/DN gel surface can differentiate the bone marrow‐derived mesenchymal stem cells (MSCs) to osteogenic cells. We used the MSCs which were harvested from the rabbit bone marrow and cultured on the polystyrene (PS) dish using the autogenous serum‐supplemented medium. First, we confirmed the properties of MSCs by evaluating colony forming unit capacity, expression of MSC markers using flow cytometry, and multidifferential capacity. Secondly, polymerase chain reaction analysis demonstrated that the HAp/DN gel surface significantly enhanced mRNA expression of the eight osteogenic markers (TGF‐β1,BMP‐2,Runx2,Col‐1, ALP,OPN,BSP, andOCN)in the cultured MSCs at 7 days than the PS surfaces (p < 0.0001), while the DN gel and HAp surfaces provided no or only a slight effect on the expression of these markers except forRunx2. Additionally, the alkaline phosphatase activity was significantly higher in the cells cultured on the HAp/DN gel surface than in the other three material surfaces (p < 0.0001). Thirdly, when the HAp/DN gel plug was implanted into the rabbit bone marrow, MSC marker‐positive cells were recruited in the tissue generated around the plug at 3 days, and Runx2 and OCN were highly expressed in these cells. In conclusion, this study demonstrated that the HAp/DN gel surface can differentiate the MSCs into osteogenic cells.
LncRNA MIR4435‐2HG functions as a ceRNA against miR‐125a‐5p and promotes neuroglioma development by upregulating TAZ
Authors: Shen Wangzhen , Zhang Jiawei , Pan Yunfeng , Jin Yun 
Published: October 2021

AbstractBackgroundExpression of the TAZ gene is closely related to the prognosis of glioma patients. We hoped to find long noncoding RNAs (lncRNAs) related to TAZ and a new target for glioma treatment.MethodsTAZ‐related genes were found by dual‐luciferase reporter gene assay, and the correlation of each gene was analyzed by the Pearson method. Human glioma cell lines U87 MG and U251 and glioma rats were used for cytology assays, and the related genes were transfected. We conducted immunohistochemistry, RT‐qPCR, Western blotting, CCK8 test, flow cytometry, transwell assays, clone formation analysis, and tumor weight measurements to verify the above relationship.ResultsWe found that miR‐125a‐5p was closely related to the TAZ gene, and the lncRNA MIR4435‐2HG was closely related to miR‐125a‐5p. Both MIR4435‐2HG‐OE and TAZ increased the expression of the TAZ gene, activated the Wnt signaling pathway, inhibited apoptosis, and promoted migration and proliferation in glioma cells. Besides, it also increased the tumor volume of gliomas in a rat model subcutaneously inoculated with glioma cells. We also found miR‐125a‐5p could block the effect of MIR4435‐2HG‐OE and TAZ.ConclusionsLncRNA MIR4435‐2HG obstructs the functions of miR‐125a‐5p and promotes neuroglioma development by upregulating the TAZ gene.