bluuebunny/crossref_metadata_2025_split huggingface dataset:
Syntaxin-1a and SNAP-25 Expression Level is Increased in the Blood Samples of Ischemic Stroke Patients.
Authors: Cappelletti Pamela , Filareti Melania , Masuelli Laura , Bei Roberto , Hassanzadeh Kambiz , Corbo Massimo , Feligioni Marco 
Published: December 2021

Abstract
The interest in peripheral blood biomarkers is growing exponentially in several neurological disorders including Ischemic Stroke (IS). The identification of neurological biochemical signs through blood sample analyses would be revolutionary allowing a better pathology diagnosis giving also information on potential recovery after specific treatments. Indeed, the increased permeability of the blood-brain barrier, following a brain infarct, allows the detection of synaptic proteins in the blood flow. In this work, we analyzed the expression levels of two synaptic proteins belonging to the Soluble N-ethylmaleimide-Sensitive Fusion Protein Attachment Protein Receptor (SNARE) family, Syntaxin (STX)-1a and Synaptosomal Associated Protein, 25 kDa (SNAP-25), in Peripheral Blood Mononuclear Cell (PBMC), serum and in Neuronal Derived Extracellular vesicles (NDEs) of IS patients, age and sex matched healthy control (HC) and younger HC (Y-HC). Interestingly, we found, for the first time, that STX-1a protein is present in the cytoplasm of PBMC. Moreover, both protein, STX-1a and SNAP-25, levels were significantly augmented in all IS patient’s blood fractions (serum, PBMCs and NDEs) compared to control subjects. Interestingly, the STX-1a blood levels always correlated with the IS clinical scales National Institutes of Health Stroke Scale (NIH-SS) and the modified Barthel Index (BI). These results prompted us to speculate that STX-1a and SNAP-25 hematic fluctuations depict the brain damage after an ischemic attack and that their hematic detection could represent a novel and accessible IS biomarkers.
Environmental DNA biomonitoring revealed species diversity of Cnidarian and Poriferan across Jakarta Bay and Seribu Islands National Park
Authors: Maqbul Ismail , Fahrezi Farrahdiba Yossan , Bakhri Ersya Nurul A , Verawati Indri , Sani Lalu M Iqbal , Subhan Beginer , Zamani Neviaty Putri , Madduppa Hawis 
Published: December 2021

Abstract
Indonesia, as a country having a unique sea lane known as the Indonesian Archipelagic Sea Lanes (ASLs), has become one of the busiest countries in the world with varied shipping activities. These actions have the ability to facilitate the transmission of species (bio-invasion). Until recently, the number of global introduced species has increased, with negative consequences for environment and the economy. Environmental DNA (eDNA) approaches for detecting the presence of invasive species are currently receiving a lot of interest as a broad approach method in ecological research. As a result, the study used the eDNA technique to compare the quantity and variety of introduced species from the Cnidaria and Porifera Phyla, as well as to characterize their invasiveness status and possible presence in the waters of Jakarta Bay. Based on data from the inside Zone of National Park (ZI) and Outside Zone of National Park (ZO), the biological community composition, richness, and diversity were assessed (ZO). The mBrave workflow generated a total of 14,275 reads from high-throughput sequencing of amplicons from two zones, with 8,917 reads in ZI and 5,358 reads in ZO. Blackfordia virginica , Cordylophora caspia , and Ectopleura crocea were among the imported species included in the invasive category, with E. crocea having the highest abundance and being detected in both zones, with a total of 1300 reads, consisting of 1253 reads in ZI and 47 reads in ZO. Based on the findings, the eDNA methodology can be used as a biomonitoring and conservation method for invasive species.
The Effect of Tourniquet Application on the Morphology and Function of Quadriceps in Patients Undergoing Total Knee Arthroplasty: A Study Protocol for a Single Blind Randomized Controlled Trial
Authors: Dong Ziyang , Li Yang , Tao Liyuan , Tian Hua 
Published: December 2021

Abstract
Background:Tourniquet is currently widely used in total knee arthroplasty to reduce intraoperative blood loss. Academic view of tourniquet application in TKA is now in dispute. Some scholars argue that tourniquet may cause quadriceps injury and bring extra side effects so they oppose the application of tourniquet. Others find that tourniquet application has no significant adverse impact on TKA patients. Regarding its advantages in reducing intraoperative blood loss, they advocate regular application of tourniquet in TKA. Quadriceps injury is considered the main cause of tourniquet side effects. There are now many high-quality trials about tourniquet application in TKA but few of them concentrate on quadriceps morphology and function.Methods:A prospective, single blind, randomized controlled trail will be adopted. The target sample is 130. Patients who meet the eligibility criteria will be randomly allocated to tourniquet group and non-tourniquet group. Primary outcomes are thickness, stiffness and function of quadriceps, which will be evaluated by ultrasound and rehabilitation tests. Secondary outcomes consist of circumference of thigh, VAS score, opioid consumption, knee function score, postoperative satisfaction score, operation time, intraoperative blood loss, perioperative blood loss, blood transfusion rate, D-Dimer, C-reactive protein, and complications. Discussion:This proposed study will contribute to improve evidence of tourniquet application in total knee arthroplasty. This will be a high-quality single blind randomized controlled trial with sufficient sample size and strict study design. It will investigate the effects of tourniquet application especially on the morphology and function of quadriceps in patients undergoing total knee arthroplasty and offer advice for tourniquet application in clinical practice.Trial registration:Chinese clinical trial registry, ChiCTR2000035097. Registered 31 July 2020, http://www.chictr.org.cn/showproj.aspx?proj=57093
Integration of anammox into the MBR process for main stream sewage treatment
Authors: Yu Dayan , Zhang Wenjie 
Published: December 2021

Abstract
The integration of anaerobic ammonia oxidation (anammox) into the membrane bioreactor (MBR) process (AX-MBR) is proposed in this study to reduce operating costs. The temperature was not controlled during the study. Anammox, denitrification, and nitrification were studied in the AX-MBR for 210 days. The reactor was fed with mainstream sewage from Guilin City, China. The results showed that AX-MBR could run with reduced dissolved oxygen (DO) concentration, and COD, NH4+-N, and total nitrogen removal were maintained or improved. The microbial analysis results demonstrated that the added anammox sludge could survive in the AX-MBR, but the sludge microbial diversity decreased. Nitrospira, Candidatus Kuenenia, and Nitrosomonas dominated the anammox sludge. In a word, the AX-MBR developed in this study could treat mainstream sewage with the appropriate management, and the operation costs are expected to reduce by decreasing the amount of aeration.
Indications of recent warm and dry summers' impact on private wells for drinking-water supply in Germany: a review of press articles
Authors: Rickert B. , Görnt A. , Vogelsang L. , Ruhl A. S. 
Published: December 2021

Abstract
Climatic changes lead to seasonal droughts with declining groundwater levels, and – especially in rural regions – private wells in the upper aquifer might fall dry. However, only limited information and no systematic administrative reporting of the extent are available for Germany yet. Therefore, a systematic analysis of newspaper articles as a promising source of information was conducted for the extraordinarily hot summers of 2018, 2019 and 2020. The results of the database searches were analysed with respect to frequency and local and regional hotspots, relations to climatic data, extent of the reported dry-fallings and emergency water supply. The analysis indicates hotspots particularly for the federal states of Saxony, where a subsidy programme for connecting to the public water supply was reissued in 2019, for Bavaria and North Rhine-Westphalia. Emergency supply was realised through various approaches. It was partly required until the winter months and did not always have drinking-water quality. As private wells are particularly vulnerable to the effects of climate change, their operators should be involved as a stakeholder group in future discussions about allocating water resources to increasingly competing uses in periods of scarcity.
Evaluation of calcium-fortified municipal water as a public health intervention to mitigate lead burdens
Authors: Rajaee Mozhgon , Dubovitskiy Ernest , Brown Victoria C. 
Published: December 2021

Abstract
Lead has adverse effects on health, society, and the economy. Lead exposure results in increased blood lead levels and storage in bones. Calcium and lead are competitively absorbed and as such calcium can be used to mitigate the body lead burden. Twenty-eight quantitative research studies were reviewed that examined lead exposure (in blood, bone, or breastmilk) and calcium intake or serum calcium to evaluate the efficacy and safety of fortifying potable water supplies with calcium to mitigate lead absorption or resorption. Eighteen of the studies reported a significant inverse relationship between biomarker lead levels and calcium intake or serum calcium. The relationship was most evident with high calcium intake, suggesting a dose-dependent relationship. An intervention with calcium-fortified water could offer an accessible source of supplemental calcium to help meet the recommended dietary allowance (RDA) and mitigate lead absorption. A concentration of 60 mg-Ca/L can supply 22.0 and 16.3% of a 1,000 mg-Ca RDA for men and women, respectively, at the recommended daily water intake.
A database system for querying of river networks: facilitating monitoring and prediction applications
Authors: Bollen Erik , Pagán Brianna R. , Kuijpers Bart , Van Hoey Stijn , Desmet Nele , Hendrix Rik , Dams Jef , Seuntjens Piet 
Published: December 2021

Abstract
The increasing availability of real-time in situ measurements and remote sensing observations have the potential to contribute to the optimisation of water resources management. Global challenges such as climate change, intensive agriculture and urbanisation put a high pressure on our water resources. Due to recent innovations in measuring both water quantity and quality, river systems can now be monitored in real time at an unprecedented spatial and temporal scale. To interpret the sensor measurements and remote sensing observations additional data, for example on the location of the measurement, and upstream and downstream catchment characteristics, are required. In this paper, we present a data management system to support flow-path-related functionality for decision making and prediction modelling. Adding meta-datasets and facilitating (near) real-time processing of sensor data questions are key concepts for the systems. The potential of the database framework for hydrological applications is demonstrated using different applications for the river system of Flanders. In one, the database framework is used to simulate the daily discharge for each segment within a catchment using a simple data-driven approach. The presented system is useful for numerous applications including pollution tracking, alerting and inter-sensor validation in river systems, or related networks.
Integrating water quality and streamflow into prediction of chemical dosage in a drinking water treatment plant using machine learning algorithms
Authors: Wang Hui , Asefa Tirusew , Thornburgh Jack 
Published: December 2021

Abstract
Understanding the relationship between raw water quality and chemical dosage is especially important for drinking water treatment plants (DWTP) that have multiple water sources where the ratio of different supply sources could change with seasons or in a matter of weeks in response to changing hydrologic conditions. In this study, the potential for deploying machine learning algorithms, including principal component regression (PCR), support vector regression (SVR) and long short-term memory (LSTM) neural network, are tested to build predictive models. These tools were used to estimate chemical dosage at a daily time-scale. Influent water quality such as pH, color, turbidity, and alkalinity, as well as chemical dosage including sulfuric acid, ferric sulfate and liquid oxygen were used to build and test these models. An 80/20 percent data split was used for training and testing model performance using correlation coefficients, relative mean square error, relative root mean square error and Nash–Sutcliffe efficiency. Results indicate, compared with PCR, both SVR and LSTM were able to capture the nonlinear relationship between chemical dose and source water quality changes and displayed higher predictive skills. These types of models have application in real-time operational support without requiring computationally expensive physics-based models.
Swine wastewater treatment by combined process of iron carbon microelectrolysis-physical adsorption-microalgae cultivation
Authors: Zhang Wenjin , Xia Rongbin , Wang Hao , Pu Shihua , Jiang Dongmei , Hao Xiaoxia , Bai Lin 
Published: December 2021

Abstract
Combined treatments were designed based on iron-carbon micro-electrolysis treatment (ICME), physical adsorption (PA) with zeolite (Z) or vermiculite (V) and microalgae cultivation (MC, Chlorella vulgaris) for removing pollutants from swine wastewater (SW): ICME + MC (IM), ICME + Z + MC (IZM) and ICME + V + MC (IVM). Results showed that the minimum total nitrogen (TN) of 43.66 mg L−1, NH4+-N of 1.33 mg−1 and total phosphorus (TP) of 0.14 mg−1 were obtained by IVM, while the minimum chemical oxygen demand (COD) was 105 mg−1 via IM. During the process of combined treatments, ICME contributed most to the removal of TN (84.52% by IZM), TP (97.78% by IVM and IZM) and COD (62.44% by IVM), and maximum NH4+-N removal (55.64%) was obtained by MC procedure in IM process. Vermiculite performed better than zeolite during all the combined treatments. Besides, the maximum cell dry weight (CDW, 0.74 g−1) of C. vulgaris was obtained by IM on day 13. The results provide an efficient integrated method for swine wastewater treatment.
Water reuse in the post-tanning process: minimizing environmental impact of leather production
Authors: Klein Rodrigo Miguel , Hansen Éverton , de Aquim Patrice Monteiro 
Published: December 2021

Abstract
Post-tanning wastewater is very diversified, as the post-tanning stage should meet the desirable properties of the leather for the final product, with low standardization of the process (compared to beamhouse and tanning). This makes post-tanning effluent reuse less feasible, and reuse in the post-tanning stage still needs to be explored. This work aims to evaluate the reuse of liquid effluents in the post-tanning process. The work methodology consisted of (i) characterization of water streams (groundwater, liquid effluent after primary treatment, and liquid effluent after secondary treatment); (ii) pilot-scale post-tanning tests using groundwater, primary effluent, and secondary effluent; (iii) characterization of the residual baths from pilot-scale tests (pH, conductivity, total solids, chemical oxygen demand, biochemical oxygen demand, chloride, hardness and oil and grease); and (iv) testing the leather obtained for total sulfated ash and organoleptic properties. Results showed that the primary effluent and the secondary effluent could be reused in pilot-scale post-tanning tests. There was an increase in the conductivity of the residual baths when liquid effluents were reused, which confirms the accumulation of salts in the effluents after their reuse.
Utracone piękno. O zniszczeniach zabytkowej architektury Zakopanego
Authors: Rupiewicz Romana 
Published: December 2021

Abstrakt:
Na terenie Zakopanego znajduje się ok. 1361 obiektów zabytkowych, z których 1180 to drewniane wille, reprezentujące styl zakopiański, powstałe w okresie od końca XIX w. do 20-lecia międzywojennego. Tak intensywne nagromadzenie drewnianych brył zakopiańskich willi ukształtowało tożsamość miejsca opartą o ład przestrzenny, stosowną skalę i proporcję. Wille stanowią wartość ze względu na historię i tradycję. Niestety, zabudowa ta jest obecnie wypierana przez inwestycje deweloperskie, które niszczą krajobraz miasta. Takie działania ułatwiają niekorzystne zapisy w miejscowych planach przestrzennych, uchwalone w latach 2009–2012. Często dochodzi do celowych podpaleń zabytków w celu pozyskania nowych gruntów inwestycyjnych. W artykule omówiono zabytkowe wille, które w ostatnich latach zostały zdewastowane lub zburzone, ukazano uwarunkowania formalno-prawne oraz mechanizmy działania. Wskazano rozwiązania, które mogą przyczynić się do lepszej ochrony konserwatorskiej zabytkowych obiektów architektury świeckiej.
Summary:
There are around 1,361 historic buildings in Zakopane, of which 1,180 are timber mountain houses of Zakopane style built from the end of the 19th century to the times of the Second Polish Republic (1918–1939). Such an accumulation of timber buildings in the local style formed an identity to the area based on spatial organization, scale and proportions. The mountain houses are valuable due to their history and tradition. Unfortunately, these structures are now superseded by investments in new developments which destroy the townscape. Such activities are facilitated by unfavorable provisions in local spatial development plans adopted during the years 2009–2012. Quite often historical buildings are deliberately set on fire in order to secure new land for investments. The article describes historic mountain houses which have been devastated or demolished in recent years. It presents the formal and legal conditions that allowed for this and the mechanisms of action. It also presents solutions which can help develop better preservation and protection of historic secular buildings.
Prediction of Age-Adjusted Mortality From Stroke in Japanese Prefectures: Ecological Study Using Search Engine Queries
Authors: Taira Kazuya , Fujita Sumio 
Published: December 2021

Background
Stroke is a major cause of death and the need for nursing care in Japan, with large regional disparities.

Objective
The purpose of this study was to clarify the association between stroke-related information retrieval behavior and age-adjusted mortality in each prefecture in Japan.

Methods
Age-adjusted mortality from stroke and aging rates were obtained from publicly available Japanese government statistics. A total of 9476 abstracts of Japanese articles related to symptoms and signs of stroke were identified in Ichushi-Web, a Japanese web-based database of biomedical articles, and 100 highly frequent words (hereafter referred to as the Stroke 100) were extracted. Using data from 2014 to 2019, a random forest analysis was carried out using the age-adjusted mortality from stroke in 47 prefectures as the outcome variable and the standardized retrieval numbers of the Stroke 100 words in the log data of Yahoo! JAPAN Search as predictive variables. Regression analysis was performed using a generalized linear mixed model (GLMM) with the number of standardized searches for Stroke 100 words with high importance scores in the random forest model as the predictive variable. In the GLMM, the aging rate and data year were used as control variables, and the random slope of data year and random intercept were calculated by prefecture.

Results
The mean age-adjusted mortality from stroke was 28.07 (SD 4.55) deaths per 100,000 for all prefectures in all data years. The accuracy score of the random forest analysis was 89.94%, the average error was 2.79 degrees, and the mean squared error was 13.57 degrees. The following 9 variables with high importance scores in the random forest analysis were selected as predictive variables for the regression analysis: male, age, hospitalization, enforcement, progress, stroke, abnormal, use, and change. As a result of the regression analysis with GLMM, the standardized partial regression coefficients (β) and 95% confidence intervals showed that the following internet search terms were significantly associated with age-adjusted mortality from stroke: male (β=−5.83, 95% CI −8.67 to −3.29), age (β=−5.83, 95% CI −8.67 to −3.29), hospitalization (β=−5.83, 95% CI −8.67 to −3.29), and abnormal (β=3.83, 95% CI 1.14 to 6.56).

Conclusions
Stroke-related search behavior was associated with age-adjusted mortality from stroke in each prefecture in Japan. Query terms that were strongly associated with age-adjusted mortality rates of stroke suggested the possibility that individual characteristics, such as sex and age, have an impact on stroke-associated mortality and that it is important to receive medical care early after stroke onset. Further studies on the criteria and timing of alerting are needed by monitoring information-seeking behavior to identify queries that are strongly associated with stroke mortality.
Improving Emergency Department Patient-Physician Conversation Through an Artificial Intelligence Symptom-Taking Tool: Mixed Methods Pilot Observational Study
Authors: Scheder-Bieschin Justus , Blümke Bibiana , de Buijzer Erwin , Cotte Fabienne , Echterdiek Fabian , Nacsa Júlia , Ondresik Marta , Ott Matthias , Paul Gregor , Schilling Tobias , Schmitt Anne , Wicks Paul , Gilbert Stephen 
Published: December 2021

Background
Establishing rapport and empathy between patients and their health care provider is important but challenging in the context of a busy and crowded emergency department (ED).

Objective
We explore the hypotheses that rapport building, documentation, and time efficiency might be improved in the ED by providing patients a digital tool that uses Bayesian reasoning–based techniques to gather relevant symptoms and history for handover to clinicians.

Methods
A 2-phase pilot evaluation was carried out in the ED of a German tertiary referral and major trauma hospital that treats an average of 120 patients daily. Phase 1 observations guided iterative improvement of the digital tool, which was then further evaluated in phase 2. All patients who were willing and able to provide consent were invited to participate, excluding those with severe injury or illness requiring immediate treatment, with traumatic injury, incapable of completing a health assessment, and aged <18 years. Over an 18-day period with 1699 patients presenting to the ED, 815 (47.96%) were eligible based on triage level. With available recruitment staff, 135 were approached, of whom 81 (60%) were included in the study. In a mixed methods evaluation, patients entered information into the tool, accessed by clinicians through a dashboard. All users completed evaluation Likert-scale questionnaires rating the tool’s performance. The feasibility of a larger trial was evaluated through rates of recruitment and questionnaire completion.

Results
Respondents strongly endorsed the tool for facilitating conversation (61/81, 75% of patients, 57/78, 73% of physician ratings, and 10/10, 100% of nurse ratings). Most nurses judged the tool as potentially time saving, whereas most physicians only agreed for a subset of medical specialties (eg, surgery). Patients reported high usability and understood the tool’s questions. The tool was recommended by most patients (63/81, 78%), in 53% (41/77) of physician ratings, and in 76% (61/80) of nurse ratings. Questionnaire completion rates were 100% (81/81) by patients and 96% (78/81 enrolled patients) by physicians.

Conclusions
This pilot confirmed that a larger study in the setting would be feasible. The tool has clear potential to improve patient–health care provider interaction and could also contribute to ED efficiency savings. Future research and development will extend the range of patients for whom the history-taking tool has clinical utility.

Trial Registration
German Clinical Trials Register DRKS00024115; https://drks.de/drks_web/navigate.do?navigationId=trial.HTML&TRIAL_ID=DRKS00024115