bluuebunny/crossref_metadata_2025_split huggingface dataset:
Total Hip Arthroplasty After Failed Less Invasive Hip Preservation Surgery for Osteonecrosis of the Femoral Head: A Systematic Review and Meta-analysis
Authors: mo liang , Li Jianxiong , Wang Zhangzheng , Huang Fayi , Xin Pengfei , Ma Chao , Zhang Jiahao , Huang Haoran , Zhou Chi , Liu Yuhao , He Wei 
Published: November 2021

Abstract
BackgroundLess invasive hip-preserving surgery (LIHP) is an effective treatment in delaying total hip arthroplasty (THA) for young patients with osteonecrosis of the femoral head (ONFH). But the success rate of it was not as effective as expected and were significantly reduced with the advancement of the diseases stages. Therefore, it is essential to analysis the impact of LIHP on subsequent THA.MethodsThe search language was restricted to Chinese and English, and the references of included studies were also searched. Chinese databases including CNKI, Wan-Fang databases and VIP, and English databases including PubMed, Embase and Cochrane library were searched by the computer from the inception of each database to 23rd May 2021. The outcome indicators were extracted from the included literature and analyzed by Cochrane Collaboration Review Manager software (RevMan version 5.4). The quality of the studies was scored using the Newcastle-Ottawa scale (NOS).ResultsA total of nine articles met the inclusion and were included in this meta-analysis, two of them were published in Chinese and the remaining studies were published in English. Results showed that the LIHP group has longer operative time (SMD=17.31, 95%CI=6.29 to 28.32, p=0.002), more intraoperative blood loss (SMD=79.90, 95%CI=13.92 to 145.87, p=0.02) and higher rate of varus or valgus femoral stem (OR=4.17, 95%CI=1.18 to 14.71, p=0.03) compared to primary THA group. The risk of intraoperative fracture was higher in the prior LIHP THA group compared with primary THA group but the difference was not statistically significant (OR=5.88, 95%CI=0.93 to 37.05, p=0.06). While there was no significant difference in cup anteversion angle (SMD=-0.10, 95%CI=-0.61 to 0.41, p=0.70), cup inclination angle (SMD=0.58, 95%CI=-0.05 to 1.22, p=0.07), postoperative Harris Hip Score (HHS) (SMD=-0.01, 95%CI=-0.43 to 0.46, p=0.96) and survivorship (OR=1.38, 95%CI=0.34 to 5.55, p=0.65) between THA groups with and without prior LIHP.ConclusionAlthough the prior LIHP increased the difficulty of the conversion to THA with longer operative time, more intraoperative blood loss, and higher rate of intraoperative fracture, it does not detrimentally affect the clinical results of subsequent THA in the mid-term following-up.
NPGREAT: Assembly of the human subtelomere regions with the use of ultralong Nanopore reads and Linked-Reads
Authors: Adam Eleni , Ranjan Desh , Riethman Harold 
Published: November 2021

Abstract
Background

Human subtelomeric DNA regulates the length and stability of adjacent telomeres that are critical for cellular function, and contains many gene/pseudogene families. Large evolutionarily recent segmental duplications and associated structural variation in human subtelomeres has made complete sequencing and assembly of these regions difficult to impossible for many loci, complicating or precluding a wide range of genetic analyses to investigate their function.
Results

We present a hybrid assembly method, NanoPore Guided REgional Assembly Tool (NPGREAT), which combines Linked-Read data with ultralong nanopore reads spanning subtelomeric segmental duplications to potentially overcome these difficulties. Linked-Read sets identified by matches with 1-copy subtelomere sequence adjacent to segmental duplications are assembled and extended into the segmental duplication regions using Regional Extension of Assemblies using Linked-Reads (REXTAL). Telomere-containing ultralong nanopore reads are then used to provide contiguity and correct orientation for matching REXTAL sequence contigs as well as identification/correction of any misassemblies (associated primarily with tandem repeats). While we focus on subtelomeres, the method is generally applicable to assembly of segmental duplications and other complex genome regions. Our method was tested for a subset of representative subtelomeres with ultralong nanopore read coverage in GM12878. 10X Linked-Read datasets with high depth of coverage and a TELL-seq Linked-Read dataset with lower depth of coverage were each combined with the ultralong nanopore reads from the same genome to provide improved assemblies. Tandem repeat regions of the short-read assemblies, which are especially prone to misassembly due to collapse of matching tandemly repeated reads, were readily identified and properly sized by comparison with the nanopore reads.
Conclusion

The NPGREAT method resulted in extension of high-quality assemblies into otherwise inaccessible segmental duplication regions near telomeres, enhancing our ability to accurately assemble human subtelomere DNA. This information will enable improved analyses of the structure, function, and evolution of these key regions.
A Terrified Sound Stress Impairs Learning And Memory Ability of Adult Female
Authors: Gao Shanfeng , Zhang Lingyu , Wang Xia , Han Lin , Xiao Xuan , Xiong Xiaofan , Li Rufeng , Ni Lei , Yang Juan 
Published: November 2021

Abstract
Stress, as an important environmental factor of mental health, cannot be ignored. The great physiological difference between males and females implies that the effects of stress may differ by gender. However, few studies have focused on the effects of stress on females. This study investigated the effects of a terrified sound stress on adult female mice.Methods: 32 adults female C57BL/6 mice were randomly divided into control group (n=16) and stress group (n=16). Sucrose preference test and open field test (OFT) were carried out to evaluate the anxiety and depression of mice. Spatial learning and memory ability were measured by Morris Water maze test (MWM). Endocrine hormones were determined by enzyme-linked immunosorbent assay (ELISA). Serum differential proteins were screened by mass spectrometry (MS). Results: Compared with control group, the sucrose preference of stress group was decreased; in MWM, the escape latency of the stress group was significantly prolonged (P<0.05), and the total swimming distance was significantly increased (P<0.05).Serum T (P<0.05), GnRH (P<0.05), FSH and LH levels decreased; thirty six differential peaks were found by MS, eight of them had high multiples of difference (> 1.2 or <0.8). Conclusion: terrified sound stress impairs spatial learning ability and mental health of adult female mice.
COVID-19 In United States: Statistical Analysis and Inferences From the Empirical Data
Authors: Rethnakar Nivedita 
Published: November 2021

Abstract
This paper investigates the mortality statistics of the COVID-19 pandemic from the United States perspective. Using empirical data analysis and statistical inference tools, we bring out several exciting and important aspects of the pandemic, otherwise hidden. Specific patterns seen in demo- graphics such as race/ethnicity and age are discussed both qualitatively and quantitatively. We also study the role played by factors such as population density. Connections between COVID-19 and other respiratory diseases are also covered in detail. The temporal dynamics of the COVID-19 outbreak and the impact of vaccines in controlling the pandemic are also looked at with suf- ficient rigor. It is hoped that statistical inference such as the ones gathered in this paper would be helpful for better scientific understanding, policy prepa- ration and thus adequately preparing, should a similar situation arise in the future.
Radiomics Nomogram Based on Spectral CT Imaging to Diagnose Osteoporosis
Authors: Yao Qianqian , Liu Mengke , Yuan Kemei , Xin Yue , Qiu Xiaoqian , Zheng Xiuzhu , Li Changqin , Duan Shaofeng , qin jian 
Published: November 2021

Abstract
Background: Osteoporosis is associated with a decrease of bone mineralized component as well as a increase of bone marrow fat. At present, there are few studies using radiomics nomogram based fat-water material decomposition (MD) images of spectral CT as an evaluation method of osteoporosis. This study aims to establish and validate a radiomics nomogram based the fat-water imaging of spectral CT in diagnosing osteoporosis.Methods: 95 patients who underwent spectral CT included T11-L2 and dual x-ray absorptiometry (DXA) were collected. The patients were divided into two groups according to T-score, normal bone mineral density (BMD) (T≥-1) and abnormally low BMD (T<-1). Radiomic features were selected from fat-water imaging of the spectral CT. Radscore was calculated by summing the selected features weighted by their coefficients. A nomogram combining the radiomics signature and significant clinical variables was built. The ROC curve was performed to evaluate the performance of the model. Finally, we used decision curve analysis (DCA) to evaluate the clinical usefulness of the model.Results: Five radiomic features based on fat-water imaging of spectral CT were constructed to distinguish abnormally low BMD from normal BMD, and its differential performance was high with an area under the curve (AUC) of 0.95 (95% CI, 0.89-1.00) in the training cohort and 0.97 (95% CI, 0.91-1.00) in the test cohort. The radiomics nomogram showed excellent differential ability with AUC of 0.96 (95%CI, 0.91-1.00) in the training cohort and 0.98 (95%CI, 0.93-1.00) in the test cohort, which performed better than the radiomics model and clinics model only. The DCA showed that the radiomics nomogram had a higher benefit in differentiating abnormally low BMD from normal BMD than the clinical model alone.Conclusion: The radiomics nomogram incorporated radiomics features and clinical factor based the fat-water imaging of spectral CT may serve as an efficient tool to identify abnormally low BMD from normal BMD well.
COVID-19 Severity among Women of Reproductive Age with Symptomatic Laboratory-Confirmed SARS-CoV-2 by Pregnancy Status – United States, Jan 1, 2020 – Sep 30, 2021
Authors: Strid Penelope , Zapata Lauren B. , Tong Van T. , Zambrano Laura D. , Woodworth Kate R. , Riser Aspen P. , Galang Romeo R. , Gilboa Suzanne M. , Ellington Sascha R. 
Published: November 2021

Abstract
Importance: Pregnant people are at increased risk for severe COVID-19 compared with nonpregnant people. Limited information is available on the severity of COVID-19 attributable to the Delta variant, the predominant variant in the United States as of late June 2021, among pregnant persons.Objective: To assess risk for severe COVID-19 by pregnancy status and time period relative to Delta variant predominance. Design: Using a cross-sectional design, we describe characteristics of symptomatic women of reproductive age (WRA) with COVID-19 and calculate adjusted risk ratios for severe disease comparing pregnant with nonpregnant WRA during the pre-Delta period (January 1, 2020 – June 26, 2021) and the Delta period (June 27, 2021 – September 30, 2021). Additionally, we calculate adjusted risk ratios for severe disease comparing the Delta period with the pre-Delta period for pregnant and nonpregnant WRA.Setting: Reports of COVID-19 in the United States occurring from January 1, 2020 ─ September 30, 2021, submitted to the CDC.Participants: Pregnant and nonpregnant women aged 15-44 years.Exposure(s): Laboratory-confirmed, symptomatic SARS-CoV-2 infection.Main Outcome(s): Severe disease: (intensive care unit [ICU] admission, receipt of invasive ventilation or extracorporeal membrane oxygenation [ECMO], and death).Results: Among 1,856,428 cases of symptomatic COVID-19 in WRA, the risk for severe disease was increased among pregnant compared with nonpregnant WRA during the pre-Delta and Delta periods. Compared with the pre-Delta period, the risk of ICU admission during the Delta period was 66% higher (adjusted risk ratio [aRR] 1.66, 95% CI: 1.34-2.06) for pregnant WRA and 23% higher (aRR 1.23, 95% CI: 1.12-1.35) for nonpregnant WRA. The risk of invasive ventilation or ECMO was higher for pregnant and nonpregnant WRA in the Delta period. During the Delta period, the risk of death was 3.40 (95% CI: 2.36-4.91) times the risk in the pre-Delta period among pregnant WRA and 1.96 (95% CI: 1.75-2.18) among nonpregnant WRA. Conclusions and Relevance: The overall risk for severe COVID-19 among WRA remains low; however, symptomatic pregnant WRA remain at increased risk for severe outcomes compared with symptomatic nonpregnant WRA during Delta variant predominance. Compared with the pre-Delta period, pregnant and nonpregnant WRA are at increased risk for severe COVID-19 in the Delta period.
Assessment of Knowledge and vaccination status towards Hepatitis B Virus among health care workers in private clinics of Addis ketema sub-city, Addis Ababa, Ethiopia.
Authors: Bekele Amanuel Kelem , Abdulwahab Hikma , Mohammedawol Ekram , Yohannes Ruth 
Published: November 2021

Abstract
Background: Hepatitis B Virus is a DNA virus of the hepadnaviridae family of viruses. It replicates within infected liver cells (hepatocytes) and may cause acute and chronic hepatitis.The hepatitis B vaccine is the mainstay of hepatitis B prevention. Since 1982, safe and effective hepatitis B virus vaccines have been commercially available. the aim of this study was to assess knowledge towards Hepatitis B Virus among health care workers in relation to their vaccination status in private clinics of Addis ketema sub-city, Addis Ababa, Ethiopia. Methods(design): A cross-sectional studywas conducted and multi-stage sampling technique was used and in total 231 healthcareworkers were enrolled in the study, which was conducted in private clinics of Addisketema sub-city. self-administered questionnaires were distributed to the health care workers. The collected data was entered intoEpi-data and analyzed using SPSS 25. Results: Two hundred thirty one healthcare workers completed and returned the questionnaires giving an overall response rate of 97%.Only 66 (28.6%) respondents reported that they received one or more doses of Hepatitis B vaccine. From these, 43 (65.2%) received three doses which was only 18.6% of the total Workers.participants with poor and moderate knowledge had equal proportion 92(39.8%) ,while only 47 (20.3%) were found to have high-level of knowledge .Having high-level of knowledge was not a statistically significant predictor of vaccination uptake. Conclusions: Hepatitis B Virus vaccination coverage and knowledge were poor among health care workers. Therefore: educational intervention and provision of vaccinationis recommended for these vulnerable workers of Addis-ketema sub city, Addis Ababa, Ethiopia.
Antibacterial and antifungal activity of intraoral products containing phthalocyanine: in vitro study
Authors: Orcina Bernardo da Fonseca , Reia Verônica Caroline Brito , Santos Caique Andrade , Peres Milena Helen , Vilhena Fabiano Vieira , Santos Paulo Sérgio da Silva 
Published: November 2021

Abstract
The present study aimed to evaluate the in vitro antiseptic efficacy of a mouthwash and a dental gel containing phthalocyanine derivatives (Pc) against bacteria and fungi frequently found in patients with ventilator-associated pneumonia. The experiment in this study was conducted following Good Laboratory Practices. The product was tested at concentrations of 0.015% (mouthwash) and 0.100% (dental gel). The contact time of the suspension test (Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Salmonella sp., Candida albicans, and Aspergillus niger) was 60 s (1 min). In this analysis, the Phtalox® Mouthwash and Dental Gel resulted in a 99.99% reduction against the tested microorganisms after 1 min of contact time in both products. The Pc-containing mouthwash and dental gel were effective against bacteria and fungi found in patients with ventilator-associated pneumonia.
An Accidental Emamectin Benzoate Poisoning In Child: A Case Report
Authors: Yadav Gopal Kumar , Mandal Krishna Chandra , Devkota Ashok Raj 
Published: November 2021

Abstract
Background: Emamectin Benzoate has high GABA (Gamma Amino Butyric Acid) receptor affinity and increase chloride membrane permeability. It is the 4'-deoxy-4'-epi-methyl-amino benzoate salt of avermectin B1 (abamectin), obtained from natural fermentation products of Streptomyces Avermitilis.Report: This case report describes the accidental poisoning of Emamectin Benzoate 5% w/ws in a female child. The child was brought to the emergency department(ED) with complaints of nausea, vomiting and abdominal pain. She consumed a packet of “LURA” (5% w/w Soluble Granule (SG) Emamectin Benzoate) supposing it as a packet of “JALJEERA” (a commonly used beverage) since there was no proper labelling. The patient was hemodynamically stable and underwent vigorous gastric lavage with normal saline, activated charcoal and coconut oil. Her blood report was normal for serum electrolytes and renal function. She was admitted in medical ward for symptomatic management and observation. She was given antiemetics, IV fluids and antacids and discharged in 2 days. In follow up after 1 week, she did not have any complain, her gastrointestinal symptoms had completely resolved and she was doing very well.Conclusion: In absence of specific antidote, vigorous gastric lavage with both activated charcoal and coconut oil improves the outcome in Emamectin Benzoate poisoning.
The PI3K/AKT/mTOR signaling pathway is aberrantly activated in primary central nervous system lymphoma and correlated with a poor prognosis.
Authors: Liu Yuanbo 
Published: November 2021

Abstract
Background

Primary central nervous system lymphoma (PCNSL) is a specific subtype of non-Hodgkin lymphoma that is highly invasive and confined to the central nervous system (CNS). The vast majority of PCNSLs are diffuse large B-cell lymphomas (DLBCLs). PCNSL is a highly heterogeneous disease, and its pathogenesis has not yet been fully elucidated. Further studies are needed to guide individualized therapy and improve the prognosis.
Methods

In this study, we detected 1) the expression of p-AKT, p-mTOR, p-S6 and p-4E-BP1 by immunohistochemistry (IHC) and Western blotting, 2) the mRNA expression by real-time qPCR and 3) the deletion of PTEN gene by immunofluorescence in situ hybridization (FISH) in order to investigate the activation status of the PI3K/AKT/mTOR signaling pathway in PCNSL. Samples of reactive hyperplasia lymphnods were used as the control group. The correlations between the clinical characteristics and prognosis of PCNSL patients and the expression of p-AKT, p-mTOR, p-S6 and p-4E-BP1 and the deletion of PTEN were assessed.
Results

The IHC results showed that the positive expression rates of p-AKT, p-mTOR, p-S6 and p-4E-BP1 in PCNSL were significantly higher in the PCNSL group than in the control group (P < 0.05). The relative mRNA expression level of MTOR in PCNSL samples was significantly increased (P = 0.013). Correlation analysis revealed that the expression of p-mTOR was correlated with that of p-AKT, p-S6, p-4E-BP1. PTEN deletion was found in 18.9% of PCNSL samples and was correlated with the expression of p-AKT (P = 0.031). Correlation analysis revealed that the PCNSL relapse rate in the p-mTOR-positive group was 64.5%, significantly higher than that in the negative group (P = 0.001). Kaplan-Meier survival analysis showed inferior progression-free survival (PFS) in the p-mTOR- and p-S6-positive groups (P = 0.002 and 0.009, respectively), and PTEN deletion tended to be related to shorter overall survival (OS) (P = 0.072). Cox regression analysis revealed p-mTOR expression as an independent prognostic factor for a shorter PFS (hazard ratio (HR) = 7.849, P = 0.046).
Conclusions

Our results suggest that the PI3K/AKT/mTOR signaling pathway is aberrantly activated in PCNSL and associated with a poor prognosis, which might indicate new therapeutic targets and prognostic factors.
Artificial neural network-based study predicts GS-441524 as a potential inhibitor of SARS COV-2 activator protein furin: A polypharmacology approach
Authors: Dhanalakshmi M , Das Kajari , Pandya Medha , Shah Sejal , Gadnayak Ayushman , Dave Sushma , Das Jayashankar 
Published: November 2021

Abstract
Furin, a pro-protein convertase, plays a significant role as a biological scissor in bacterial, viral, and even mammalian substrates which in turn decides the fate of many viral and bacterial infections along with the numerous ailments caused by cancer, diabetes, inflammations, and neurological disorders. In the wake of the current pandemic caused by the virus SARS COV-2, furin has become the center of attraction for researchers as the spike protein contains a polybasic furin cleavage site. In the present work, we have searched for novel inhibitors against this interesting human target from FDA-approved antivirals. To enhance the selection of new inhibitors we employed Kohonen’s artificial neural network-based self-organizing maps for ligand-based virtual screening. Promising results were obtained which can help in drug repurposing and network pharmacology studies addressing the errors generated due to promiscuity/polypharmacology. We found 15 existing FDA antivirals having the potential to inhibit furin. Among these, six compounds have targets on many important human proteins (LDLR, FCGR1A, PCK1, TLR7, DNA, and PNP) also. These 15 drugs inhibiting furin could be studied in patients infected with many viruses including SARS COV-2. We also propose two promising candidate FDA drugs GS-441524 and Grazoprevir (MK-5172) for repurposing as inhibitors of furin. The best results were observed with GS-441524.
Clinical, Immunological and Genetic Characterization of Patients With X-linked Agammaglobulinemia in Costa Rica
Authors: Ivankovich-Escoto Gabriela , Danielian Sylvia , Morera Estela , Barboza Elexandra , Atmella Ivannia , Silva Sandra , Santamaría Carlos , Porras Oscar 
Published: November 2021

Abstract
X-linked agammaglobulinemia is caused by mutations in the gene encoding Bruton tyrosine kinase. It produces an arrest in the maturation and differentiation of B cells with very low levels of all immunoglobulins isotypes. The aim of the study was to characterize the clinical, immunological and genetic defect in patients with XLA in Costa Rica. Sixteen cases were identified over a period of 30 years, a case every 2 years, approximately. Three patients were asymptomatic and diagnosis was made by family history. the average age of onset of symptoms was 1.46 years-old (0.08-6.1). Six patients (44%) had onset of symptoms before 1 year of age and 12 (81%) patients before 5 years of age. The average age of diagnosis was 3.63 years-old (0.17-13, SD 3.51 years-old the average time between the onset of symptoms and the diagnosis was 2.5 years (2.5 months to 12 years, SD 3 years). Initial reason to study the patients was recurrent infection, family history of XLA, arthritis and neutropenia. Four patients had pneumonia and two had suppurative lung disease. Nine patients had recurrent infection: acute otitis media, sinusitis, mastoiditis and recurrent diarrhea. Three patients presented with arthritis. Neutropenia as an isolated event was not identified in any case. All patients receive monthly IVIG and no deaths were reported. Three new likely pathogenic/pathogenic variants in BTK gene have been described in our population. This is the first report of XLA Costa Rican patients and their BTK mutations.