bluuebunny/crossref_metadata_2025_split huggingface dataset:
A phylogeny of the genus Limia (Teleostei: Poeciliidae) suggests a single-lake radiation nested in a Caribbean-wide allopatric speciation scenario
Authors: Spikes Montrai , Rodríguez-Silva Rodet , Bennett Kerri-Ann , Bräger Stefan , Josaphat James , Torres-Pineda Patricia , Ernst Anja , Havenstein Katja , Schlupp Ingo , Tiedemann Ralph 
Published: November 2021

AbstractObjectiveThe Caribbean is an important global biodiversity hotspot. Adaptive radiations there lead to many speciation events within a limited period and hence are particularly prominent biodiversity generators. A prime example are freshwater fish of the genusLimia, endemic to the Greater Antilles. Within Hispaniola, nine species have been described from a single isolated site, Lake Miragoâne, pointing towards extraordinary sympatric speciation. This study examines the evolutionary history of theLimiaspecies in Lake Miragoâne, relative to their congeners throughout the Caribbean.ResultsFor 12Limiaspecies, we obtained almost complete sequences of the mitochondrial cytochromebgene, a well-established marker for lower-level taxonomic relationships. We included sequences of six furtherLimiaspecies from GenBank (total N  = 18 species). Our phylogenies are in concordance with other published phylogenies ofLimia. There is strong support that the species found in Lake Miragoâne in Haiti are monophyletic, confirming a recent local radiation. Within Lake Miragoâne, speciation is likely extremely recent, leading to incomplete lineage sorting in the mtDNA. Future studies using multiple unlinked genetic markers are needed to disentangle the relationships within the Lake Miragoâne clade.
Recent strategies for enhancing the therapeutic efficacy of stem cells in wound healing
Authors: Zhao Yongqing , Wang Min , Liang Feng , Li Jiannan 
Published: November 2021

AbstractSkin wound healing is a multi-stage process that depends on the coordination of multiple cells and mediators. Chronic or non-healing wounds resulting from the dysregulation of this process represent a challenge for the healthcare system. For skin wound management, there are various approaches to tissue recovery. For decades, stem cell therapy has made outstanding achievements in wound regeneration. Three major types of stem cells, including embryonic stem cells, adult stem cells, and induced pluripotent stem cells, have been explored intensely. Mostly, mesenchymal stem cells are thought to be an extensive cell type for tissue repair. However, the limited cell efficacy and the underutilized therapeutic potential remain to be addressed. Exploring novel and advanced treatments to enhance stem cell efficacy is an urgent need. Diverse strategies are applied to maintain cell survival and increase cell functionality. In this study, we outline current approaches aiming to improve the beneficial outcomes of cell therapy to better grasp clinical cell transformation.
Physico-chemical characteristics and aflatoxins production of Atractylodis Rhizoma to different storage temperatures and humidities
Authors: Liu Qiutao , Jiang Lingling , Xiao Lihe , Kong Weijun 
Published: November 2021

AbstractThis study aimed to investigate the characteristics, moisture contents, chemical fingerprints changes and aflatoxins accumulation ofAtractylodis rhizomaduring storage, further to determine the optimum temperature and relative humidity conditions. Based on the suitable temperature (20–40 °C) and relative humidity (80–95%), 13 different temperature and humidity conditions were set up by the central composite design-response surface methodology (CCD-RSM) forAspergillus flavus. After inoculation withAspergillus flavusby artificial infection,A. rhizomasamples were stored under normal conditions and 13 different temperature and relative humidity levels. By taking the changes of characteristics, the contents of moisture, chemical fingerprints and aflatoxins as the evaluation indexes forA. rhizomawith or withoutAspergillus flavusfungi to optimize the optimal storage conditions. After storage for 10 days, the color ofA. rhizomawas deepened, the water content and chemical composition increased, and some unknown components were detected. The susceptible condition for aflatoxins production inA. rhizomawas identified at temperature 22–37 °C and relative humidity over 87.5%. Thus, the suitable storage conditions forA. rhizomashould be controlled at temperature below 20 °C and relative humidity less than 85%. This paper screened out the optimum temperature and humidity for the storage ofA. rhizoma. Then, the storage specification forA. rhizomawas proposed, lying technical and data support for the scientific preservation of other food or herbs.
Prevalence and predictors of different patterns of hypertension among adults aged 20–60 years in rural communities of Southeast Nigeria: a cross-sectional study
Authors: Ayogu Rufina N. B. , Ezeh Mmesoma G. , Okafor Adaobi M. 
Published: November 2021

Abstract
Background
Hypertension, a major cardiovascular disease risk factor exists several years without symptoms. Few data exist on prevalence and predictors of hypertension among apparently healthy Nigerian adults. This makes it difficult for policy-makers to concentrate efforts to control emerging health burden of the disease. This study assessed prevalence and predictors of isolated systolic hypertension (ISH), isolated diastolic hypertension (IDH) and combined systolic and diastolic hypertension (CSDH).

Methods
Cross-sectional survey design was employed in the study of 517 adult participants (20–60 years) in a rural setting. Selection of the respondents was through multistage sampling which involved systematic, proportionate and simple random sampling. Data on socio-demographic characteristics, blood pressure, height, weight, and waist circumference were collected. Frequencies, T-test, analysis of variance and Chi square were used in statistical analysis. Bivariate and multivariate logistic regressions were used to evaluate variables associated with different patterns of hypertension with significance accepted at P < 0.05. Frequencies, percentages, crude and adjusted odd ratios were reported. Statistical Product and Service Solutions version 21.0 was used in statistical analysis.

Results
ISH (10.6%), IDH (18.2%) and CSDH (37.8%) were observed among the participants. ISH was less likely among 20–29 year-olds (adjusted odds ratio (aOR) = 0.35, 95% confidence interval (C.I.) = 0.13–0.94), 30–39 year-olds (aOR = 0.30, 95% C.I. = 0.11–0.82) and those with abdominal obesity (aOR = 0.12, 95% C.I. = 0.03–0.56). Participants who perceived their health status as good (aOR = 3.80, 95% C.I. = 1.29–11.18) and excellent (aOR = 5.28, 95% C.I. = 1.54–18.07) were respectively 3.80 and 5.28 times more likely to have ISH. Those with secondary education had significantly higher likelihood for IDH (aOR = 2.05, 95% = 1.02–4.14) whereas self-perceived poor health status (aOR = 0.24, 95% C.I. = 0.09–0.65), absence of obesity (aOR = 0.10, 95% C.I. = 0.01–0.81) and general obesity (aOR = 0.35, 95% C.I. = 0.17–0.72) were associated with reduced risk for IDH. Secondary (aOR = 0.60, 95% C.I. = 0.36–0.99) and tertiary (aOR = 0.49, 95% C.I. = 0.28–0.85) education were associated with reduced risk for CSDH but combined obesity (aOR = 4.39, 95% C.I. = 2.25–8.58) increased the risk for CSDH by 4.

Conclusion
ISH, IDH and CSDH were problems among the adults with age, obesity, self-perception of good/excellent health status and low education level as significant predictors. Health and nutrition education to prevent comorbidities and cerebrovascular accidents are recommended.
Design and clinical developments of aptamer-drug conjugates for targeted cancer therapy
Authors: Kim Do-Hun , Seo Jin-Myung , Shin Kyung-Ju , Yang Su-Geun 
Published: November 2021

Abstract

Background
Aptamer has been called “chemical antibody” which displays the specific affinity to target molecules compared to that of antibodies and possesses several therapeutic advantages over antibodies in terms of size, accessibility to synthesis, and modification. Based on the attractive properties, aptamers have been interested in many directions and now are emerged as new target-designed cancer drug.

Main body
Currently, new types of aptamers have been reported and attracted many scientists’ interesting. Due to simplicity of chemical modification and ready-made molecular engineering, scientists have developed newly designed aptamers conjugated with a wide range of therapeutics, aptamer-drug conjugates; ApDCs, from chemotherapy to phototherapy, gene therapy, and vaccines. ApDCs display synergistic therapeutic effects in cancer treatment.

Conclusion
In this paper, we reviewed various kinds of ApDCs, i.e., ApDC nucleotide analogs, ApDC by drug intercalation, and ApDC by using chemical linker. Current data prove these ApDCs have sufficient potential to complete clinical development soon. Advanced technology of cancer drug delivery and combination treatment of cancers enables aptamer and conjugated drug (ApDCs) efficient means for targeted cancer treatment that reduces potential toxicity and increases therapeutic efficacy.
Thorium and fetal neural tube defects: an epidemiological evidence from large case-control study
Authors: Wang Bin , Pang Yiming , Zhang Yali , Zhang Le , Ye Rongwei , Yan Lailai , Li Zhiwen , Ren Aiguo 
Published: November 2021

Abstract
Background
Thorium is ubiquitous in the environment and its relationship with birth defects is still under discussion. This study aimed to investigate the associations of maternal exposure to thorium with risk of neural tube defects (NTDs) by using a case–control study, as well as the relationship between thorium exposure and the indoor air pollution from coal combustion.

Methods
This study was conducted in 11 local healthcare hospitals during 2003–2007 in Shanxi and Hebei provinces, China. A total of 774 mothers were included as participants who delivering 263 fetuses with NTDs including 123 with anencephaly, 115 with spina bifida, 18 with encephalocele, and 7 other NTD subtypes (cases), and 511 health fetuses without NTDs (controls). Their hair samples were collected as close as to the occipital posterior scalp, of which those grew from 3 months before to 3 months after conception was cut to measure the thorium concentration by inductively coupled plasma-mass spectrometry.

Results
We found a higher hair thorium concentration in the total NTD cases with 0.901 (0.588–1.382) ng/g hair [median (inter-quartile range)] than that in the controls with a value of 0.621 (0.334–1.058) ng/g hair. Similar results were found for the three concerned NTD subtypes. Maternal hair thorium concentration above its median of the controls was associated with an increased risk of the total NTDs with an adjusted odds ratio of 1.80 [95% confidence interval (CI), 1.23–2.63)] by adjusting for all confounders. There was obvious dose-response relationship between maternal hair thorium concentration and the risk of total NTDs, as well as their two subtypes (i.e. anencephaly and spina bifida). Maternal hair thorium concentration was positive associated with their exposure level to indoor air pollution from coal combustion during cooking.

Conclusions
Overall, our findings revealed that maternal periconceptional thorium exposure was associated with the risk of NTDs in North China. Reducing the coal usage in the household cooking activities may decrease maternal thorium exposure level.

Graphical abstract
Kinetic studies of Ni(II) ions adsorption from aqueous solutions using the blast furnace slag (BF slag)
Authors: Chouchane Toufik , Khireddine Ouahida , Boukari Atmen 
Published: November 2021

AbstractIn this work, we used the blast furnace slag for the nickel adsorption in aqueous solution. The physico-chemical characterization showed that the BF slag consists mainly of the silica, lime, and alumina. The specific surface area of the BF slag grains is of the order of 275.8 m2/g. The optimum elimination parameters are the agitation speed 200 rpm, pH 4.5, the adsorption temperature 20 °C, and particle size between 200 and 500 μm. The adsorption capacity and the efficiency of nickel removal by the BF slag after 90 min of agitation are respectively 53.58 mg/g and 92.7%.The experimental adsorption data showed that the pseudo-second-order model was the most appropriate in nickel adsorption kinetics; the adsorption isotherm could be described well by the Langmuir model indicating that the process was monolayer, and intra-particle diffusion is not the sole mechanism involved in this process. Thermodynamic study showed that the Ni(II) elimination by BF slag process is spontaneous, exothermic, and less entropic.
Experimental evidence of nonlinear avalanche dynamics of energetic particle modes
Authors: Yu L. M. , Zonca F. , Qiu Z. Y. , Chen L. , Chen W. , Ding X. T. , Ji X. Q. , Wang T. , Wang T. B. , Ma R. R. , Yuan B. S. , Shi P. W. , Li Y. G. , Liu L. , Shi Z. B. , Cao J. Y. , Dong J. Q. , Liu Yi , Yang Q. W. , Xu M. 
Published: November 2021

Abstract
Recent observations in HL-2A tokamak give new experimental evidences of energetic particle mode (EPM) avalanche. In a strong EPM burst, the mode structure propagates radially outward within two hundred Alfvén time, while the frequency of the dominant mode changes self-consistently to maximize wave-particle power exchange and mode growth. This suggests that significant energetic particle transport occurs in this avalanche phase, in agreement with theoretical framework of EPM convective amplification. A simplified relay runner model yields satisfactory interpretations of the measurements. The results can help understanding the nonlinear dynamics of energetic particle driven modes in future burning plasmas, such as ITER.
Periodic wave solution of the Kundu-Mukherjee-Naskar equation in birefringent fibers via the Hamiltonian-based algorithm
Authors: Wang Kang-Jia , Zhu Hong-Wei 
Published: November 2021

Abstract
The Kundu-Mukherjee-Naskar equation can be used to address certain optical soliton dynamics in (

) dimensions. In this paper, we aim to find its periodic wave solution by the Hamiltonian-based algorithm. Compared with the existing results, we found a good agreement, which strongly proves the correctness of the proposed method. Finally, the numerical results are presented in the form of 3-D and 2-D plots. The results in this work are expected to shed a bright light on the study of the periodic wave solution in physics.
KATNB1 is a master regulator of multiple katanin enzymes in male meiosis and haploid germ cell development
Authors: Dunleavy Jessica E. M , O'Connor Anne E. , Okuda Hidenobu , Merriner D. Jo , O'Bryan Moira K. 
Published: November 2021

ABSTRACT
Katanin microtubule-severing enzymes are crucial executers of microtubule regulation. Here, we have created an allelic loss-of-function series of the katanin regulatory B-subunit KATNB1 in mice. We reveal that KATNB1 is the master regulator of all katanin enzymatic A-subunits during mammalian spermatogenesis, wherein it is required to maintain katanin A-subunit abundance. Our data shows that complete loss of KATNB1 from germ cells is incompatible with sperm production, and we reveal multiple new spermatogenesis functions for KATNB1, including essential roles in male meiosis, acrosome formation, sperm tail assembly, regulation of both the Sertoli and germ cell cytoskeletons during sperm nuclear remodelling, and maintenance of seminiferous epithelium integrity. Collectively, our findings reveal that katanins are able to differentially regulate almost all key microtubule-based structures during mammalian male germ cell development, through the complexing of one master controller, KATNB1, with a ‘toolbox’ of neofunctionalised katanin A-subunits.
Snazarus and its human ortholog SNX25 modulate autophagic flux
Authors: Lauzier Annie , Bossanyi Marie-France , Larcher Raphaëlle , Nassari Sonya , Ugrankar Rupali , Henne W. Mike , Jean Steve 
Published: November 2021

ABSTRACT
Macroautophagy, the degradation and recycling of cytosolic components in the lysosome, is an important cellular mechanism. It is a membrane-mediated process that is linked to vesicular trafficking events. The sorting nexin (SNX) protein family controls the sorting of a large array of cargoes, and various SNXs impact autophagy. To improve our understanding of their functions in vivo, we screened all Drosophila SNXs using inducible RNA interference in the fat body. Significantly, depletion of Snazarus (Snz) led to decreased autophagic flux. Interestingly, we observed altered distribution of Vamp7-positive vesicles with Snz depletion, and the roles of Snz were conserved in human cells. SNX25, the closest human ortholog to Snz, regulates both VAMP8 endocytosis and lipid metabolism. Through knockout-rescue experiments, we demonstrate that these activities are dependent on specific SNX25 domains and that the autophagic defects seen upon SNX25 loss can be rescued by ethanolamine addition. We also demonstrate the presence of differentially spliced forms of SNX14 and SNX25 in cancer cells. This work identifies a conserved role for Snz/SNX25 as a regulator of autophagic flux and reveals differential isoform expression between paralogs.
Tuned muscle and spring properties increase elastic energy storage
Authors: Mendoza Elizabeth , Azizi Emanuel 
Published: November 2021

ABSTRACT
Elastic recoil drives some of the fastest and most powerful biological movements. For effective use of elastic recoil, the tuning of muscle and spring force capacity is essential. Although studies of invertebrate organisms that use elastic recoil show evidence of increased force capacity in their energy loading muscle, changes in the fundamental properties of such muscles have yet to be documented in vertebrates. Here, we used three species of frogs (Cuban tree frogs, bullfrogs and cane toads) that differ in jumping power to investigate functional shifts in muscle-spring tuning in systems using latch-mediated spring actuation (LaMSA). We hypothesized that variation in jumping performance would result from increased force capacity in muscles and relatively stiffer elastic structures, resulting in greater energy storage. To test this, we characterized the force–length property of the plantaris longus muscle–tendon unit (MTU), and quantified the maximal amount of energy stored in elastic structures for each species. We found that the plantaris longus MTU of Cuban tree frogs produced higher mass-specific energy and mass-specific forces than the other two species. Moreover, we found that the plantaris longus MTU of Cuban tree frogs had higher pennation angles than the other species, suggesting that muscle architecture was modified to increase force capacity through packing of more muscle fibers. Finally, we found that the elastic structures were relatively stiffer in Cuban tree frogs. These results provide a mechanistic link between the tuned properties of LaMSA components, energy storage capacity and whole-system performance.
Thermal performance curves for aerobic scope in a tropical fish (Lates calcarifer): flexible in amplitude but not breadth
Authors: Scheuffele Hanna , Rubio-Gracia Francesc , Clark Timothy D. 
Published: November 2021

ABSTRACT
Aerobic metabolic scope is a popular metric to estimate the capacity for temperature-dependent performance in aquatic animals. Despite this popularity, little is known of the role of temperature acclimation and variability in shaping the breadth and amplitude of the thermal performance curve for aerobic scope. If daily thermal experience can modify the characteristics of the thermal performance curve, interpretations of aerobic scope data from the literature may be misguided. Here, tropical barramundi (Lates calcarifer) were acclimated for ∼4 months to cold (23°C), optimal (29°C) or warm (35°C) conditions, or to a daily temperature cycle between 23 and 35°C (with a mean of 29°C). Measurements of aerobic scope were conducted every 3–4 weeks at three temperatures (23, 29 and 35°C), and growth rates were monitored. Acclimation to constant temperatures caused some changes in aerobic scope at the three measurement temperatures via adjustments in standard and maximum metabolic rates, and growth rates were lower in the 23°C-acclimated group than in all other groups. The metabolic parameters and growth rates of the thermally variable group remained similar to those of the 29°C-acclimated group. Thus, acclimation to a variable temperature regime did not broaden the thermal performance curve for aerobic scope. We propose that thermal performance curves for aerobic scope are more plastic in amplitude than in breadth, and that the metabolic phenotype of at least some fish may be more dependent on the mean daily temperature than on the daily temperature range.
Dispersive coupling between MoSe2 and an integrated zero-dimensional nanocavity
Authors: Rosser David , Gerace Dario , Chen Yueyang , Liu Yifan , Whitehead James , Ryou Albert , Andreani Lucio C. , Majumdar Arka 
Published: November 2021

Establishing a coherent interaction between a material resonance and an optical cavity is a necessary first step to study semiconductor quantum optics. Here we report on the signature of a coherent interaction between a two-dimensional excitonic transition in monolayer MoSe2 and a zero-dimensional, ultra-low mode volume (V
m
∼ 2(λ/n)3) on-chip photonic crystal nanocavity. This coherent interaction manifests as a dispersive shift of the cavity transmission spectrum, when the exciton-cavity detuning is decreased via temperature tuning. The exciton-cavity coupling is estimated to be ≈6.5 meV, with a cooperativity of ≈4.0 at 80 K, showing our material system is on the verge of strong coupling. The small mode-volume of the resonator is instrumental in reaching the strongly nonlinear regime, while on-chip cavities will help create a scalable quantum photonic platform.
Das Römische Heerwesen: auch im 21. Jahrhundert Gegenstand intensiver Forschung
Authors: Wesch-Klein Gabriele 
Published: November 2021

History of the Roman army – in a broad sense – was one of the pillars of Géza Alföldy’s scholarly studies and investigations. His inspiring interpretations of various inscriptions had a fundamental impact on the shape of contemporary science. He was particularly interested in such issues as religion of the Roman military, the origins of soldiers, the rules of promotion and the careers of officers with senatorial and equestrian lineage.
The article offers an overview of major studies in history of the Roman army published in the recent years. There is no doubt that many had been inspired by the work and writings of Géza Alföldy. Although it is merely a subjective selection, the list of publications appended to the article attests to the intensity of such research, showing that it truly flourishes.
Strictly speaking, Géza Alföldy did not come up with any novel research problems as far as the Roman military was concerned. He would address those which had been present in science, as it is documented by works of such scholars as Alfred von Domaszewski, Konrad Kraft, Emil Ritterling, Eric Birley or Arthur and Ernst Stein. Nonetheless, Géza Alföldy understood the essence and appreciated the need to pursue those studies in greater depth while utilizing epigraphy to the broadest possible extent. Quite rightly, he considered inscriptions to be the foremost sources in that respect. Guided by hic saxa loquntur as his motto, Alföldy determined the shape of methodology employed in studies of the Roman army through his work. The ordinary soldiers and officers became genuine individuals, which in a sense enabled one to gain insight into their personal lives, professional careers, family ties, and religious beliefs. Thus far, research in the two first decades of the 21st century follows the same path. It may only be desired that, as a result, the current state of our knowledge will be substantially enriched.