bluuebunny/crossref_metadata_2025_split huggingface dataset:
Population Distribution of Wild Radish (Raphanus Raphanistrum L.) Weed and Soil Depth of Seed Bank Across Different Land-Use Types in Horro District, Western Oromia, Ethiopia
Authors: Shone Geleta , Wakoya Fikiru , Jaleta Zerihun , Legese Hirpha 
Published: June 2021

Abstract
Background: Wild radish (Raphanus raphanistrum L.) is an invasive weed in Ethiopia and hurting different land use types in the Horro district of the study area and fact, more research has not been done yet on the weed is highly infested and persisted for three decades. Therefore, this study was conducted to assess the weeds flora and seed bank status across different land use types in the Horro district of western Oromia, Ethiopia. Three locations and from each location five different land use were purposively selected and a 1m2 area of quadratic sampling techniques was used to record weed flora. From each quadrat, soil samples were taken having two soil depth levels (0-15cm and >15-30cm) to evaluate the soil seed bank of weeds. Results: The data of wild radish weed in different location and different land use types both field survey and greenhouse experiments were found a statistically significant difference (p<0.05) among the target action of land use types but there was found no significant difference among soil depths. More all less, the highest wild radish population was recorded from grazing land land followed by wheat crop land and among the locations, Gitilo Dale has the highest weed density. Among the weed flora, again wild radish was highly dominated weed species than the other weed species in all land use types and locations. From soil seed bank status of soil depth, high weed population was recorded at 0-16cm of second season weed emergence from pot and Gitilo Dale was the highest infected location at this season, while barley crop land was the highest infected among land use types in first season of weed emergence from pot. Among the weed species were recorded from soil depth, again wild radish was highly dominated in both seasons. The population of weed flora per quadrat was directly proportion with population recorded from soil depth that emerged from pot. Conclusions: This study has highly verified that this area has highly infected by wald radish (Raphanus raphanistrum) and there could be a source of weed seed. Generally, the concerned bodies should have to be given attention which should need immediate intervention strategies to mitigate the negative impacts of weed otherwise the area will be out of production soon. Therefore, an extensive integrated weed management strategies should be applied in all phases of cropping systems to sustain environment and reduce this weed in the future.
Quantifying Radial Growth Response of Pinus Yunnanensis to Climate Change and Drought Event at Different Altitudes and Ages in the Jinsha River Basin
Authors: Su Jianrong , Shen Jiayan , Li Shuaifeng , Huang Xiaobo , Liu Wande , Lang Xuedong 
Published: June 2021

Abstract
Background

The relative influence of climate change and drought events on tree growth at different altitude and tree ages remains insufficiently understood in the Jinsha River Basin, southwest China, limiting prediction of forest adaptability to high-frequency droughts and climate change. We conducted a dendroecological study to explore and quantify the dominant climate factors that determining radial growth of Pinus yunnanensis trees of different ages and at different altitudes, to evaluate their resilience to drought events.
Results

Radial growth of P. yunnanensis at high elevations is typically limited by low temperatures, the explanatory rate of temperature factors on growth increased from 23.6–59.7% with altitude. Tree growth at low elevations is more sensitive to moisture factors, the explanatory rate of moisture factors on growth decreased from 76.4–40.3% with altitude. The young and mature trees are more prone to moisture factors than middle-age and near-mature trees, the young and near-mature trees are more prone to temperature factors than middle-age and mature trees. The older trees usually showed less drought resistance and recovery than the young and middle-age trees. The resistance and recovery of P. yunnanensis weakened with the increased frequency of drought events. Tree resistance and resilience was highly dependent on the average pre-drought growth, whereas the recovery showed weak or no significant relationships with average pre-drought growth.
Conclusion

Our study demonstrates that radial growth of P. yunnanensis trees showed age- and altitude-specific demand for energy and moisture. P. yunnanensis trees at different altitudes and ages are differentially adapted to varying levels of climate stress and display different strategies to withstand the effects of drought with altitude and ages.
Integrated Analysis of Tumor‑Associated Macrophage Infiltration and Prognosis in Ovarian Cancer
Authors: tan qianxia , liu huining , xu jie , mo yanqun , wu chenchun , dai furong 
Published: June 2021

Abstract
BackgroundMost ovarian cancer patients with poor prognosis and immune microenvironment play a vital role in the progression of ovarian cancer. We aim to develop a tumor-associated macrophage related gene (TAMRGs) prognostic signature that can stratify and predict overall survival for ovarian cancer.MethodsWe acquired single cell and bulk transcriptome raw data of ovarian cancer from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO). The immune landscape was identified in primary and ascites of ovarian cancer. CIBERSORT deconvolution algorithm, Weighted gene co-expression network analysis (WGCNA), univariate cox analysis, LASSO algorithm, and multivariate cox analysis were performed for the identification of TAMRG and the development of prognostic signature.Results We identified inter-and intra-patient heterogeneity for immune infiltration cells at a single-cell resolution. Tumor infiltration macrophages showed immunosuppressive characteristics with an M2 phenotype. T cell CD4 memory activated, mast cell activated, neutrophils and macrophages M2 were negatively correlated with overall survival, while macrophages M1 was positively correlated. A total of 219 TAMRGs were identified and a novel 6-gene signature (TAP1, CD163, VSIG4, IGKV4-1, CD3E, and MS4A7) with superior prognostic independence was established.ConclusionsThe TAMRG-based signature is expected to be a promising target for prognosis and treatment response of ovarian cancer.
Ketamine used in the therapy of depressive disorders impacts protein profile, proliferation rate and phagocytosis resistance of enterococci
Authors: Jarzembowski Tomasz Artur , Daca Agnieszka , Cubała Wiesław J. , Bronk Marek , Naumiuk Łukasz 
Published: June 2021

Abstract

Background: Ketamine is known to cause rapid anti-depressive effect. Additionally, it has been also proved that at high concentrations ketamine inhibits bacterial growth. It is also widely known that even sub-inhibitory concentration of chemicals, as concentration of ketamine used in therapy of depression, may change bacterial properties, including their virulence. The knowledge about possible influence of ketamine on bacterial commensals seems to be essential, as the mechanism of ketamine’s action in depression is believed to result also from its’ anti-inflammatory activity. In the current study we aimed to evaluate the in vitro influence of ketamine on proliferation rate, phagocytosis resistance and toxicity of enterococci. Results: The studied enterococcal strains were isolated as etiological agents of infection and collected in the Department of Medical Microbiology, Medical University of Gdansk. To measure metabolic activity of Enterococcus faecalis 10µM of CFDA-SE was added to bacterial suspension. The number of bacterial cells and fluorescence of particles were determined using FACSVerse flow cytometer. Additionally, for the determination of phagocytosis resistance, THP-1 human monocytes cell line from ATCC was used. Suspension of monocytes which engulfed bacteria was then stained with propidium iodide to determine cells’ membrane permeability and to evaluate cytotoxicity of enterococci. The result of the study proved diverse influence of therapeutic concentration of ketamine on Enterococci. In 23.1% of strains both proliferation rate and metabolism activity were inhibited. This group of strains was more susceptible to phagocytosis and had lower cytotoxicity than in culture without ketamine. Different response of isolates to ketamine was also visible in changes of protein profile determined by MALDI TOF. Conclusions: The analysis of bacteria at early stage of growth curve demonstrate the bacterial diversity in response to ketamine and let us set the hypothesis that microbiome susceptibility to ketamine may be one of the elements which should be taken into consideration when planning the successful pharmacotherapy of depression.
An open source extrusion bioprinter based on the E3D motion system and tool changer to enable FRESH and multi-material bioprinting
Authors: Engberg Adam , Stelzl Christina , Eriksson Olle , O'Callaghan Paul , Kreuger Johan 
Published: June 2021

Abstract
Bioprinting is increasingly used to create complex tissue constructs for an array of research applications, and there are also increasing efforts to print tissues for transplantation. Bioprinting may also prove valuable in the context of drug screening for personalized medicine for treatment of diseases such as cancer. However, the rapidly expanding bioprinting research field is currently limited by access to bioprinters. To increase the availability of bioprinting technologies we present here an open source extrusion bioprinter based on the E3D motion system and tool changer to enable high-resolution multi-material bioprinting. As proof of concept, the bioprinter is used to create collagen constructs using freeform reversible embedding of suspended hydrogels (FRESH) methodology, as well as multi-material constructs composed of distinct sections of laminin and collagen. Data is presented demonstrating that the bioprinted constructs support growth of cells either seeded onto printed constructs or included in the bioink prior to bioprinting. This open source bioprinter is easily adapted for different bioprinting applications, and additional tools can be incorporated to increase the capabilities of the system.
Optimization of the electrochemical oxidation of textile wastewater by graphite electrodes by response surface methodology and artificial neural network
Authors: Saleh Mohammed , Yildirim Rabia , Isik Zelal , Karagunduz Ahmet , Keskinler Bulent , Dizge Nadir 
Published: June 2021

Abstract
In this study, electrochemical oxidation of combed fabric dyeing wastewater was investigated using graphite electrodes. The response surface methodology (RSM) was used to design the experiments via the central composite design (CCD). The planned experiments were done to track color changes and chemical oxygen demand (COD) removal. The experimental results were used to develop optimization models using RSM and the artificial neural network (ANN) and they were compared. The developed models by the two methods were in good agreement with the experimental results. The optimum conditions were found at 150 A/m2, pH 5, and 120 min. The removal efficiencies for color and COD reached 96.6% and 77.69%, respectively. The operating cost at the optimum conditions was also estimated. The energy and the cost of 1 m3 of wastewater required 34.9 kWh and 2.58 US$, respectively. The graphite electrodes can be successfully utilized for treatment of combed fabric dyeing wastewater with reasonable cost.
PERFIL EPIDEMIOLÓGICO DE ACIDENTES COM ANIMAIS PEÇONHENTOS NO ESTADO DO MARANHÃO
Authors: Cordeiro Eduardo Costa , Almeida Joelson dos Santos , Da Silva Thiago Sousa 
Published: June 2021

Introdução: Considerados como um problema de saúde pública, os acidentes causados por animais peçonhentos (ofídios, escorpiões, aranhas e dentre outros) são responsáveis por um considerável número de agravos à população mundial. Na região avaliada, estudos acerca do tema são inexistentes, havendo a necessidade do levantamento de dados, uma vez que, informações atualizadas são de fundamental importância para que ações de vigilância epidemiológica sejam empregadas com maior segurança. Objetivo: Descrever o perfil epidemiológico dos acidentes com animais peçonhentos no estado do Maranhão. Metodologia: Trata-se de um estudo ecológico, descritivo com abordagem quantitativa. Os dados foram coletados no SINAN/DATASUS dos casos notificados de acidentes com animais peçonhentos. Resultados: Constou-se uma maior incidência de notificações de acidentes em 2019 e nos meses de Fevereiro (10,14%), Março (9,96%) e Maio (9,89%). No que tange ao perfil das vítimas houve uma predominância no sexo masculino (68,16%), adultos jovens de 20 a 39 anos (36,24%) e autodeclarados pretos (75,03%). No que diz respeito à gestação a imensa maioria não se aplica a categoria gestante (77,71%). Em relação às características do acidente destacou-se o acidente escorpiônico (55,97%), seguido pelos acidentes com aranhas (30,66%) e ofídicos (4,09%). O tempo decorrido entre o acidente e o atendimento foi predominante no intervalo de 1 a 3 h após o acidente (34,37%). Dos acidentes ocorridos 61,31% foram classificados como leves, 82,02% evoluíram clinicamente com cura e 0,6% para óbito. Conclusão: Com base nos dados apresentados, torna-se evidente a importância dos acidentes causados por animais peçonhentos para a saúde pública, assim aponta-se que medidas intersetoriais entre a vigilância epidemiológica, a secretaria do meio ambiente e o centro de controle de zoonoses são essenciais para a diminuição da mortalidade por acidentes com animais venenosos.
A CONTRIBUIÇÃO DO PROJETO “INFOJOVEM” NA EDUCAÇÃO EM SAÚDE DE ADOLESCENTES
Authors: Bomfim Ana Marlusia Alves , Leão Melro Ana Flávia 
Published: June 2021

Introdução: A adolescência é um período do ciclo da vida balizado pela influência da vulnerabilidade, onde o adolescente passa por fases de crescimento e desenvolvimento no âmbito físico e intelectual, e merece uma atenção especial por parte de todos aqueles que lidam com o mesmo. Objetivo: Descrever uma experiência vivenciada por discentes e docentes, na participação de um projeto de extensão sobre a prevenção de saúde dos adolescentes. Metodologia: Trata-se de um estudo descritivo, tipo relato de experiência, acerca das atividades realizadas por discentes e docentes do curso de medicina do Centro Universitário Tiradentes- UNIT/AL, entre os meses de abril e junho de 2019, com adolescentes de ambos os sexos e faixa etária entre 12 e 18 anos, residentes em bairros da periferia de Maceió. Foi utilizada como técnica a roda de conversa e recursos audiovisuais, como facilitadores do processo da aprendizagem. Resultados: As atividades experiênciadas possibilitaram a formação de vínculos, facilitando a integração do público alvo à proposta metodológica utilizada durante os encontros. Assim, percebe-se que houve um processo de aprendizagem profícuo, onde os adolescentes se sentiram entusiasmados a serem protagonistas na vivência dos encontros realizados sobre a temática em tela. Conclusões: A prática educativa com adolescentes provenientes de uma conjuntura social vulnerável, oportunizou aos discentes e docentes reflexões da agradável tarefa de serem facilitadores do conhecimento. Além de perceberem que é um desafio lidar com um grupo que se encontra em formação e que possui diversas indagações e conflitos pertinentes a própria idade.

Palavras-Chave: educação em saúde; saúde do adolescente; saúde da comunidade.
AÇÕES DO ENFERMEIRO NA PREVENÇÃO DE INFECÇÕES ASSOCIADAS AO USO DE CATETERES EM UNIDADE DE TERAPIA INTENSIVA: REVISÃO INTEGRATIVA
Authors: Teixeira Rayssa , Da Silva Miriane Virlane , Da Silva Edla Maria , Neves Santos Ruth Stephanie , Souza Vanderley Érica Catia , De Carvalho Silva Cintia , Lopes Oliveira Diego Augusto 
Published: June 2021

Introdução: As infecções relacionadas ao uso de cateteres na Unidade de Terapia Intensiva (UTI) representam uma realidade de altos índices, que consolidam-se como problema de saúde pública e sobre o qual a equipe de enfermagem se caracteriza como potencial agente transformador a partir do estabelecimento de medidas específicas de cuidados com esses dispositivos. Objetivo: Elucidar as ações do enfermeiro na prevenção de infecções associadas ao uso de cateteres na UTI. Metodologia: Estudo descritivo do tipo revisão integrativa realizado nos anos de 2015 a 2019, no período de março a maio nas bases de dados MEDLINE, LILACS e BDENF. Construído a partir de 6 etapas. A análise dos estudos se deu pela leitura de títulos, resumos, e artigos completos, uma vez que estes respondessem o objetivo determinado. Posteriormente a síntese dos resultados encontrados foi apresentada em tabelas, sendo seguida pela discussão a partir de categorias temáticas identificadas através da Técnica de Análise de Conteúdo. Resultados: Através dos estudos selecionados, observou-se uma gama de boas práticas que podem ser utilizadas pela enfermagem na garantia da qualidade do cuidado com os dispositivos, sendo elas referentes aos aspectos técnicos do manuseio ou não, como higiene das mãos, uso de EPI’s, dupla checagem, estratégias de desinfecção, boa comunicação entre profissionais e métodos para trabalhar fatores interferentes no manejo dos cateteres, como estresse, insegurança e esgotamento. Conclusões: Sugere-se a intensificação da padronização de bundles e protocolos de medidas, bem como melhoria na abrangência destes, para que considerem às várias etapas de manejo com os cateteres. Percebe-se a necessidade de entender um conjunto de fatores que interferem na adesão e disseminação das boas práticas de enfermagem nesse contexto. Enfatiza-se a necessidade de estudos que detalhem de modo aprofundado a realização das boas práticas, e discutam fatores que afetam o sucesso da implantação das mesmas.
Palavras-Chave: Infecções Relacionadas a Cateter; Unidades de Terapia Intensiva; Cuidados de Enfermagem.
APRENDIZADOS E REFLEXÕES ADVINDOS DE ATIVIDADE EXTENSIONISTA DE EDUCAÇÃO EM SAÚDE EM CENTROS DE EDUCAÇÃO INFANTIL
Authors: Oliveira Tomaz Ferraresso Lucas Fernando , Britto Codato Lucimar Aparecida 
Published: June 2021

Introdução: Atividade extensionista configura-se como poderoso instrumento para a formação integral e contextualizada do estudante. Favorece agregação, aplicação e absorção de múltiplos conhecimentos na comunidade. O projeto Ações de Educação em Saúde e de Educação Continuada para Profissionais do SUS da Universidade Estadual de Londrina, desenvolve atividades de educação em saúde vinculadas às demandas apresentadas pelos Centros de Educação Infantil que atua. Objetivos: Relatar o processo ensino-aprendizagem e os desafios vivenciados em uma atividade extensionista e correlacioná-los com a curricularização da extensão. Metodologia: Trata-se de um estudo descritivo do tipo relato de experiência extensionista, desenvolvido por estudantes do terceiro ao décimo período e professores de Odontologia. O estudo refere-se ao período de 2018 e 2019. Resultados: Foram realizadas ações de educação em saúde sobre adoção de hábitos saudáveis relativos à saúde geral das crianças, pais e cuidadores por meio da elaboração e distribuição de folders. Para os integrantes do projeto, esta demanda foi desafiadora em relação à definição de estratégias, planejamento, conciliação de horários e adoção de recursos que favorecessem a comunicação com a população. Esta experiência extensionista, remeteu à reflexão sobre a amplitude das potencialidades da curricularização da extensão, porque alia a relevância social e favorece a formação integral do estudante. Porém, o alcance dos objetivos das ações extensionistas e da curricularização da extensão, dependem do envolvimento e dedicação dos docentes e estudantes. Conclusões: Atividades extensionistas e a curricularização da extensão são relevantes para o ensino-aprendizagem e para cumprimento do compromisso social das universidades. São complexas e multifacetadas, para além dos projetos apresentados e da implementação de legislações e diretrizes educacionais.
Palavras-Chave: Relações Comunidade-Instituição; Educação Superior; Educação em Saúde.
Thymic Immunosuppressive Pentapeptide (TIPP) Shown Anticancer Activity in Breast Cancer and Chronic Myeloid Leukemia Both In Vitro and In Vivo
Authors: Ijaz Muhammad , Shahbaz Muhammad , Jiang Wenjie , Shi Yikang , Guo Xiuli , Wang Fengshan 
Published: June 2021

Aim:
Being the common cause and major burden of deaths globally, timely cancer management
is crucial.

Background:
Thymic immunosuppressive pentapeptide (TIPP) is a novel pentapeptide originally
obtained from calf thymic immunosuppressive extract. Previously, TIPP has been proved to suppress
the allergic and inflammatory responses in allergic mice via blocking MAP kinases/NF-κB
signaling pathways.

Objective:
In this study, in vitro anticancer activity of TIPP was tested on two different types of
cancers using MCF-7 and K562 cell lines.

Methods:
Tumor xenograft models for breast cancer and chronic myeloid leukemia were designed.
In vivo anticancer activity of TIPP was investigated on both cancer types. The liver and tumor tissues
of the mice were preserved for immunohistochemistry analysis.

Results:
In vitro anticancer activity of TIPP showed significant inhibition on cell viability of both
breast cancer and chronic myeloid leukemia. In vivo anticancer effect of TIPP in both types of cancer
models further proved the potent anticancer nature of TIPP. Immunohistochemistry analysis assured
that TIPP is a safe drug for normal organs such as the liver.

Conclusion:
Our present study revealed that TIPP is a potent anticancer drug and an important
treatment option for various diseases. Further work is needed to test the flexible and proficient activity
of the novel peptide.
Effect of Nitrate on Gene and Protein Expression of Nitric Oxide Synthase
Enzymes in Insulin-Sensitive Tissues of Type 2 Diabetic Male Rats
Authors: Shokri Majid , Jeddi Sajad , Faridnouri Hassan , Khorasani Vajiheh , Kashfi Khosrow , Ghasemi Asghar 
Published: June 2021

Background and Objective:
Decreased nitric oxide (NO) bioavailability contributes to
the pathophysiology of type 2 diabetes mellitus (T2DM). This study aims to determine the effects
of nitrate (NO3
–) on gene and protein expression of NO synthase (NOS) enzymes in the liver,
soleus muscle (SM), and epididymal adipose tissue (eAT) of rats with T2DM.

Methods:
Twenty-eight male rats were divided into 4 groups: Control, diabetes, control+NO3
–, and
diabetes+NO3
– (n = 7/each group). NO3
– was administered for 6 months, and mRNA and protein
levels of NOS enzymes were measured at the end of the study.

Results:
mRNA and protein levels of inducible NOS (iNOS) were higher in the liver (475% and
73%), SM (271% and 43%), and eAT (543% and 24%) of rats with T2DM. In the case of the endothelial
NOS (eNOS), diabetic rats had lower mRNA and protein levels in the liver (26% and
24%) and SM (60% and 62%) and lower mRNA level (30%) in eAT. mRNA and protein levels of
neural NOS (nNOS) were lower in SM (69% and 73%) and eAT (25% and 31%) of rats with
T2DM. NO3
– administration restored disrupted iNOS and eNOS expressions to their near normal
values in all the studied tissues; NO3
– also increased nNOS mRNA and protein levels in SM and
eAT but decreased nNOS protein level in the liver.

Conclusion:
Long-term NO3
– administration restored disrupted expression of NOS enzymes in the
liver, SM, and eAT of rats with T2DM; these findings partly explain the beneficial metabolic effects
of nitrate in T2DM.