bluuebunny/crossref_metadata_2025_split huggingface dataset:
Physicochemical and microbiological changes of bovine blood due to high hydrostatic pressure treatment
Authors: Csehi B. , Salamon B. , Csurka T. , Szerdahelyi E. , Friedrich L. , Pásztor-Huszár K. 
Published: June 2021

AbstractBovine blood samples were treated with high hydrostatic pressure (HHP) to examine the changes that may occur in the blood related to its colour, microbiological characteristics, protein denaturation, and dynamic viscosity. Pressure treatments were carried out from 100 to 600 MPa in 100 MPa scale up, with 5 min holding time. The blood samples were treated with anticoagulant (EDTA) to eliminate the possible measurement distorting effects. We found that 2 log reduction in the microbial load could be achieved with a pressure treatment above 400 MPa. According to the protein denaturation measurements (DSC), blood proteins were resistant to pressure treatment, even at 300–400 MPa a substantial part of proteins remained in native state. The colour of the samples got darker with the rising pressure, however, visible colour change was observed only above 400 MPa. It can be established, that the HHP treatment was suitable to increase the microbiological stability of blood, without significantly changing its techno-functional properties.
A comparative study of HPLC and UV spectrophotometric methods for oseltamivir quantification in pharmaceutical formulations
Authors: Gungor Serdar , Bulduk Ibrahim , Sultan Aydın Beyza , Ilikci Sagkan Rahsan 
Published: June 2021

Abstract
Oseltamivir is an antiviral drug and is used in the treatment of all influenza viruses. It is the most effective antiviral option against all influenza viruses that can infect humans. UV and LC methods have been developed and validated according to ICH guidelines for various parameters like selectivity, linearity, accuracy, precision, LOD and LOQ, robustness for the quantitative determination of oseltamivir in pharmaceutical formulations. LC method has been performed using reverse phase technique on a C-18 column with a mobile phase consisting of 20 mM potassium dihydrogen phosphate solution and acetonitrile (60:40, v/v) at 25 °C. The mobile phase flow rate was 1.2 mL min−1. For the determination of oseltamivir, UV spectrum has been recorded between 200 and 800 nm using methanol as solvent and the wavelength of 215 nm has been selected. Both methods have demonstrated good linearity, precision and recovery. No spectral and chromatographic interferences from the capsule excipients were found in UV and LC methods. In both methods, correlation coefficients were greater than 0.999 within a concentration range of 10–60 mg mL−1 using UV and LC. Intra-day and inter-day precision with low relative standard deviation values were observed. The accuracy of these methods was within the range 99.85–100.17% for LC and from 99.26 to 100.70% for UV. Therefore UV and LC methods gave the most reliable outcomes for the determination of oseltamivir in pharmaceutical formulation.
Heart rate dynamics and lactate following high-intensity race-pace continuous vs interval workouts in highly trained athletes
Authors: Martinez-Navarro I. , Montoya A. , Mateo-March M. , Blasco-Lafarga C. 
Published: June 2021

AbstractPurposeThe present study aimed to compare the physiological responses of high-intensity race-pace continuous vs. interval workouts commonly used in middle-distance athletics, by means of analyzing post-exercise cardiac autonomic regulation and lactate.MethodsNineteen highly-trained 800-m male runners were asked to run a 600-m race-pace continuous workout and a 2 × 4 × 200-m interval training, counterbalanced and randomized within one week of difference. Blood lactate jointly with linear and nonlinear heart rate dynamics were assessed during the immediate 15-min recovery. Age-category (Under23-Senior vs. Juvenile-Junior) was considered as an inter-subject factor.ResultsPeak lactate was higher following the interval training (15.51 ± 0.99 vs 13.83 ± 1.77 mmol L−1; P < 0.05) whereas lactate removal was almost nonexistent 15 min after both workouts (between 0 and 16%). Vagal modulation (ln RMSSD and lnRMSSD to RR ratio) remained significantly depressed at the end of recovery following both workouts, although the alteration was larger following the interval training. Detrended Fluctuation Analysis evidenced a more random HR behavior (DFA1 closer to 0.5) during the first 9 min of recovery after the interval training, whereas no significant change was observed in heart rate complexity (SampEn). Neither were differences found in post-exercise lactate and HR dynamics as a function of age-category.ConclusionsHigh-intensity workouts commonly used in middle-distance athletics, both race-pace continuous and intervallic approaches, induce a large depression of vagal modulation in highly trained runners, although interval trainings appear to induce even a greater alteration of both linear and nonlinear HR dynamics and a higher post-exercise peak lactate.
Approaches of combining a 3D-printed elastic structure and a hydrogel to create models for plant-inspired actuators
Authors: Rodriguez Nadia , Bastola Anil K. , Behl Marc , Soffiatti Patricia , Rowe Nick P. , Lendlein Andreas 
Published: June 2021

Abstract
Inspired by the interesting functional traits of a climbing cactus, Selenicereus setaceus, found in the forest formations of Southeastern Brazil, we formulated a hypothesis that we can directly learn from the plants to develop multi-functional artificial systems by means of a multi-disciplinary approach. In this context, our approach is to take advantage of 3D-printing techniques and shape-memory hydrogels synergistically to mimic the functional traits of the cactus. This work reports on the preliminary investigation of cactus-inspired artificial systems. First, we 3D-printed soft polymeric materials and characterized them, which defines the structure and is a passive component of a multi-material system. Second, different hydrogels were synthesized and characterized, which is an active component of a multi-material system. Finally, we investigated how the hydrogel can be integrated into the 3D-printed constructs to develop artificial functional systems.

Graphic abstract
Importance of the traditional food systems for First Nations adults living on reserves in Canada
Authors: Batal Malek , Chan Hing Man , Fediuk Karen , Ing Amy , Berti Peter , Sadik Tonio , Johnson-Down Louise 
Published: June 2021

AbstractObjectiveTo describe the traditional food (TF) systems of First Nations in Canada, including intake, barriers and promoters.MethodsThe First Nations Food, Nutrition and Environment Study is a cross-Canada participatory study of First Nations adults below the 60thparallel that obtained data for communities excluded from other national studies. A food frequency questionnaire was used to establish frequency of TF intake (number of days in a year) to allow comparisons across ecozones/regions in Canada. Grams of TF intake were also calculated using frequency multiplied by average portions from 24-h recalls. Closed- and open-ended questions attempted to identify some of the key barriers and concerns regarding TF access and use. Multivariable analyses were run to determine what factors are associated with increased TF consumption.ResultsAcross communities, there is a strong preference by adults to have TF in the diet more often. Consumption of land animals was most frequently reported in most ecozones except for the Pacific Maritime and Mixedwood Plains, where fish and plants, respectively, were more frequently consumed. First Nations identified structural and environmental challenges such as development, government regulations and climate change, along with household barriers such as insufficient capital for equipment and transportation, lack of time and absence of a hunter in the household. Multivariable analyses revealed that the highest intake of TF occurred in the Taiga Plains ecozone, and for older individuals and men.ConclusionIdentifying solutions that empower First Nations at all levels is required to overcome the multiple challenges to the inclusion of TF in the diet.
First Nations households living on-reserve experience food insecurity: prevalence and predictors among ninety-two First Nations communities across Canada
Authors: Batal Malek , Chan Hing Man , Fediuk Karen , Ing Amy , Berti Peter R. , Mercille Genevieve , Sadik Tonio , Johnson-Down Louise 
Published: June 2021

Abstract
Objective
To describe the prevalence of food insecurity in First Nations households across Canada while identifying barriers and enablers to traditional food (TF) consumption.

Methods
The First Nations Food, Nutrition and Environment Study is a cross-Canada participatory study of on-reserve First Nations from 2008 to 2018. The Household Food Security Survey Module was used to capture income-related challenges experienced by First Nations households. Households were classified as food secure, or marginally, moderately, or severely food insecure. Barriers and enablers to TF access and use were identified describing the Indigenous experience.

Results
Almost half of on-reserve First Nations households were food insecure and the prevalence was higher than that for non-Indigenous households in Canada. On-reserve food insecurity prevalence was higher in western regions of Canada. First Nations households with children experienced greater food insecurity than those without children. More adults experienced severe food insecurity than children. Most adults would like to have more TF in their diet but state that factors such as financial and household constraints, industrial activities, government regulations, climate change, and fear of contamination impede greater access. Food costs were substantially higher in remote First Nations communities, but remoteness was not associated with food security in multivariable analysis.

Conclusion
Existing systems have been unsuccessful in curbing the food insecurity in First Nations households. Improving food security hinges on achieving Indigenous Food Sovereignty, the key to long-term conservation and stewardship of the land and the co-management of these by Indigenous Peoples. Studies investigating the feasibility of increasing TF from an Indigenous perspective are required.
Levels of metals and persistent organic pollutants in traditional foods consumed by First Nations living on-reserve in Canada
Authors: Chan Hing Man , Singh Kavita , Batal Malek , Marushka Lesya , Tikhonov Constantine , Sadik Tonio , Schwartz Harold , Ing Amy , Fediuk Karen 
Published: June 2021

Abstract
Objectives
First Nations may have a higher risk of contaminant exposure from the consumption of traditional foods. The objective of this study was to measure concentrations of metals and organochlorines in traditional foods commonly consumed by First Nations in Canada and estimate the risk from dietary exposure.

Methods
Data were collected from the participatory First Nations Food, Nutrition and Environment Study (2008–2018). Traditional food samples were collected by community members and concentrations of metals and organochlorines were measured. The population-weighted mean daily contaminant intake from traditional food items was estimated. Hazard quotients (HQs) were calculated by dividing contaminant intake with the toxicological reference values (TRVs).

Results
A total of 2061 food samples (different parts and organs) from 221 species were collected. The highest concentrations of cadmium were found in the kidneys of land mammals: moose kidney was the most significant contributor to intake. The meat of land mammals and birds had the highest lead concentrations and were the most significant contributors to intake. Arsenic was highest in seaweed, and prawn was the most significant contributor. Mercury and methyl mercury were highest in harp seal meat, with walleye/pickerel contributing most to intake. Harp seal meat also had the highest p,p′-DDE and PCB concentrations, and ooligan grease and salmon were the most significant contributors to intake. The percentage of adults eating traditional food who exceeded the TRVs was 1.9% for cadmium, 3.7% for lead, 13.6% for arsenic, 0.7% for mercury, and 0% for p,p′-DDE and PCBs. All median HQs, and most 95th percentile HQs, were less than 1.

Conclusion
These results can be used as a baseline of contaminant levels and exposure in traditional foods for future monitoring programs and to support risk assessment programs.
Phase relations in the CuI-SbSI-SbI3 composition range of the Cu–Sb–S–I quaternary system
Authors: Mammadli Parvin R. , Gasymov Vagif A. , Dashdiyeva Ganira B. , Babanly Dunya M. 
Published: June 2021

The phase equilibria in the Cu-Sb-S-I quaternary system were studied by differential thermal analysis and X-ray phase analysis methods in the CuI-SbSI-SbI3 concentration intervals. The boundary quasi-binary section CuI-SbSI, 2 internal polythermal sections of the phase diagram, as well as, the projection of the liquidus surface were constructed. Primary crystallisation areas of phases, types, and coordinates of non- and monovariant equilibria were determined. Limited areas of solid solutions based on the SbSI (b-phase) and high-temperature modifications of the CuI (α1- and α2- phases) were revealed in the system. The formation of the α1 and α2 phases is accompanied by a decrease in the temperatures of the polymorphic transitions of CuI and the establishment of metatectic (3750C) and eutectoid (2800C) reactions. It was also shown, that the system is characterised by the presence of a wide immiscibility region that covers a significant part of theliquidus surface of the CuI and SbSI based phases
Consistent Pitch Height Forms: A commentary on Daniel Muzzulini's contribution Isaac Newton's Microtonal Approach to Just Intonation
Authors: Noll Thomas 
Published: June 2021

This text revisits selected aspects of Muzzulini's article and reformulates them on the basis of a three-dimensional interval space E and its dual E*. The pitch height of just intonation is conceived as an element h of the dual space. From octave-fifth-third coordinates it becomes transformed into chromatic coordinates. The dual chromatic basis is spanned by the duals a* of a minor second a and the duals b* and c* of two kinds of augmented primes b and c. Then for every natural number n a modified pitch height form hn is derived from h by augmenting its coordinates with the factor n, followed by rounding to nearest integers. Of particular interest are the octave-consitent forms hn mapping the octave to the value n. The three forms hn for n = 612, 118, 53 (yielding smallest deviations from the respective values of n h) form the Muzzulini basis of E*. The respective transformation matrix T* between the coordinate representations of linear forms in the Muzzulini basis and the dual chromatic basis is unimodular and a Pisot matrix with the dominant eigen-co-vector very close to h. Certain selections of the linear forms hn are displayed in Muzzuli coordinates as ball-like point clouds within a suitable cuboid containing the origin. As an open problem remains the estimation of the musical relevance of Newton's chromatic mode, and chromatic modes in general. As a possible direction of further investigation it is proposed to study the exo-mode of Newton's chromatic mode
Identification of pathogenic fungi in garlic bulbs during storage and in the root zone during plant growth
Authors: Filyushin M. A. , Danilova O. A. , Seredin T. M. 
Published: June 2021

Relevance and methods. Losses of agricultural crops are associated not only with the development of diseases during the growing season, but also during post-harvest storage. Garlic is a popular vegetable and aromatic crop in world. Significant losses in garlic yield during cultivation and storage are associated with fungal pathogens, the most harmful of which are representatives of the genus Fusarium. In the Moscow region, the defeat of garlic by Fusarium occurs annually, but with varying intensity. At the Federal Scientific Vegetable Center (FSVC), it was shown that rot and wilting of garlic plants is caused by a complex of pathogenic fungi, including mainly different species of Fusarium. At the same time, the ratio of Fusarium species in the pathogenic complex changes from year to year, new Fusarium species and their races are registered. The aim of this study was to identify fungal phytopathogens causing dry rot of garlic cloves during post-harvest storage. To carry out the work, garlic bulbs of cultivars Dubkovsky and Strelets were taken from the FSVC storage.Results. As a result of visual examination, cloves with symptoms of dry rot were identified. The diseased cloves tissues were plated on potato dextrose agar to obtain fungal colonies. Analysis of the morphological and cultural characteristics of fungal isolates, as well as the nucleotide sequences of four DNA regions (ITS spacers, genes EF1α, RPB1, and RPB2) showed that the causative agent of dry rot of garlic cloves is the pathogenic fungus Fusarium proliferatum. In addition, in the field, identification was carried out based on the analysis of the sequences of spacers ITS and the EF1αgene of phytopathogenic fungi inhabiting the root zone of garlic plants. As a result, two species of fungi of the genus Fusarium (F. proliferatum and F. oxysporum f. sp. cepae), as well as the species Rhizoctonia solani, Volutella rosea, and Ceratobasidium sp. were found in the root zone of garlic cultivars.
Identification of <i>Pseudomonas cichorii</i> (Swingle 1925) Stapp 1928 in hydroponic culture of lettuce in Russia
Authors: Tesic S. , Pakina E. N. , Ignatov A. N. 
Published: June 2021

Relevance.Lettuce (Latin: Lactúca satíva) is a species of annual herbaceous plant in the genus Lettuce of the Asteraceae family. As a vegetable crop, it is cultivated everywhere in the world, and its hydroponic cultivation technology has received special development in recent years. One of the common pathogens of lettuce is Pseudomonas cichorii, causing bacterial diseases of several important cultivated plants. In this regard, the study of the occurrence of this pathogen is important.Material and methodology.The study was conducted on the basis of the Department of Agrobiotechnology of the ATI of RUDN University. The samples were provided by a commercial manufacturer of lettuce grown on a flow-through hydroponic line under conditions of minimal microbial contamination. The study of phytopathogenic bacteria includes a number of stages: isolation of bacteria on semi-selective culture media and obtaining a pure culture of bacteria; setting a test for pathogenicity (virulence); studying the phenotypic properties of bacteria; determining the taxonomic position of the isolated strains by molecular methods. All studies were conducted in accordance with the standard methods of identification of phytopathogenic bacteria.Results.As a result of the work, the distribution of the species Pseudomonas cichorii in the hydroponic culture of lettuce in the Russian Federation was confirmed. Although, according to the EPPO database, P. cichorii was first described in Russia in 1965 by microbiological methods, but isolated bacteria are not available in microbiological collections to confirm this conclusion with appropriate diagnostic methods. Twelve isolates of P. cichoriiwere studied by a biochemical and phytopathological tests, and four isolates (01, 04, 06, and 12) that showed the greatest aggressiveness on host plants and tobacco leaves were identified by DNA sequencing of the 16S rRNA gene fragment. The obtained DNA fragments showed a high similarity (99-100%) with the sequences of P. cichoriifrom the Genebank. Evaluation of the virulence of the isolated isolates on a number of other cultivated plants, and the uniformity of their biochemical characteristics showed that they represent a group of bacteria specialized in lettuce.
Agrochemical and water-physical properties of sod-podzolic soil with drip irrigation of a fruit nursery
Authors: Dubenok N. N. , Gemonov A. V. , Lebedev A. V. , Efimov O. E. , Prokhorov A. A. 
Published: June 2021

Relevance. The use of irrigation is one of the directions of intensification of fruit growing. Drip irrigation is considered one of the promising methods of irrigation, which provides the creation of the most favorable conditions for the growth and development of plants, makes it possible to supply irrigation water directly to their roots and allows the use of automation tools in the irrigation process. The results of ongoing scientific research show that changes in the water-physical and agrochemical properties of soils can be observed on irrigated lands. For the Central Region of the Non-Chernozem Zone of Russia, studies on the influence of drip irrigation on soil properties are fragmentary.Methods. Field studies were conducted on the territory of the educational experimental farm of the Michurinsky Garden fruit growing laboratory of the Russian State Agrarian University - Moscow Timiryazev Agricultural Academy. A two-factor experience in the study of different ranges of moisture for the formation of varietal plum seedlings grafted on plum tree stock was laid in the spring of 2018. Before setting up the experiment, organic fertilizers were introduced in the form of horse manure with sawdust in the amount of 100 t/ha. Soil moisture was controlled using tensiometers, calibrated based on the data of the thermostat-weight method. Irrigation rates were set in such a way as to increase the moisture content by 20% of the lowest moisture capacity. The identification of the main agrochemical and water-physical properties of the soil in the experimental plot was carried out according to generally accepted methods and techniques.Results. The data obtained on the characteristics of the water-physical and agrochemical properties of sod-podzolic soil make it possible to reasonably design the irrigation regime, reclamation and agrotechnical measures. The results of the study show that the local irrigation of soils with sparing irrigation rates with the application has led to certain changes in some soil parameters. However, the obtained research results suggest that in quantitative terms, these changes are not yet significant.