bluuebunny/crossref_metadata_2025_split huggingface dataset:
Reduction of Matrix Metallopeptidase 13 and Promotion of Chondrogenesis by Zeel T in Primary Human Osteoarthritic Chondrocytes
Authors: Sanchez Christelle , Hemmer Kathrin , Krömmelbein Natascha , Seilheimer Bernd , Dubuc Jean-Emile , Antoine Christophe , Henrotin Yves 
Published: May 2021

Objectives: Zeel T (Ze14) is a multicomponent medicinal product. Initial preclinical data suggested a preventive effect on cartilage degradation. Clinical observational studies demonstrated that Ze14 reduced symptoms of osteoarthritis (OA), including stiffness and pain. This study aimed to explore these effects further to better understand the mode of action of Ze14 on human OA chondrocytes in vitro.Methods: Primary chondrocytes were obtained from the knees of 19 OA patients and cultured either as monolayers or in alginate beads. The cultures were treated with 20% or 10% (v/v) Ze14 or placebo. For RNA-seq, reads were generated with Illumina NextSeq5000 sequencer and aligned to the human reference genome (UCSC hg19). Differential expression analysis between Ze14 and placebo was performed in R using the DESeq2 package. Protein quantification by ELISA was performed on selected genes from the culture medium and/or the cellular fractions of primary human OA chondrocyte cultures.Results: In monolayer cultures, Ze14 20% (v/v) significantly modified the expression of 13 genes in OA chondrocytes by at least 10% with an adjusted p-value &lt; 0.05: EGR1, FOS, NR4A1, DUSP1, ZFP36, ZFP36L1, NFKBIZ, and CCN1 were upregulated and ATF7IP, TXNIP, DEPP1, CLEC3A, and MMP13 were downregulated after 24 h Ze14 treatment. Ze14 significantly increased (mean 2.3-fold after 24 h, p = 0.0444 and 72 h, p = 0.0239) the CCN1 protein production in human OA chondrocytes. After 72 h, Ze14 significantly increased type II collagen pro-peptide production by mean 27% (p = 0.0147). For both time points CCN1 production by OA chondrocytes was correlated with aggrecan (r = 0.66, p = 0.0004) and type II collagen pro-peptide (r = 0.64, p = 0.0008) production. In alginate beads cultures, pro-MMP-13 was decreased by Ze14 from day 7–14 (from −16 to −25%, p &lt; 0.05) and from day 17–21 (−22%, p = 0.0331) in comparison to controls.Conclusion: Ze14 significantly modified the expression of DUSP1, DEPP1, ZFP36/ZFP36L1, and CLEC3A, which may reduce MMP13 expression and activation. Protein analysis confirmed that Ze14 significantly reduced the production of pro-MMP-13. As MMP-13 is involved in type II collagen degradation, Ze14 may limit cartilage degradation. Ze14 also promoted extracellular matrix formation arguably through CCN1 production, a growth factor well correlated with type II collagen and aggrecan production.
Assessing Intertemporal Socioeconomic Inequalities in Alcohol Consumption in South Africa
Authors: Fontes Marx Mayara , London Leslie , Harker Nadine , Ataguba John E. 
Published: May 2021

Background: This paper assesses changes in the socioeconomic inequality in alcohol consumption by exploring whether alcohol consumption (current and binge drinkers) is more prevalent among the wealthier (pro-rich) or poorer (pro-poor) group over time.Methods: Data come from the 2008, 2010/11, 2012, and 2014/15 waves of the National Income Dynamics Study (NIDS). Various equity stratifiers (sex, age, race, and rural/urban) are used to analyze the prevalence of alcohol consumption and to investigate differences in socioeconomic inequalities. Changes in socioeconomic inequality in alcohol consumption between 2008 and 2014/15 were also assessed using the concentration index.Results: Current drinkers were more concentrated among richer South Africans, while binge drinkers were concentrated among the poorer population. For current drinkers, irrespective of sex, race, age, and urban, socioeconomic inequality in alcohol consumption had become less pro-rich between 2008 and 2014/15; while inequality in binge drinking, outside of the Asian/Indian and rural categories, had become less pro-poor between 2008 and 2014/15.Conclusion: The results show evidence that binge drinking is a bigger problem among those of low-SES, young individuals, male and African populations. This paper concludes that the SA government should continue to push forward policies aiming to reduce the prevalence of binge drinking.
Predicting Progression of COVID-19 Infection to Prioritize Medical Resource Allocation: A Novel Triage Model Based on Patient Characteristics and Symptoms at Presentation
Authors: Tu Yuexing , Zhou Xianlong , Shao Lina , Zheng Jiayin , Wang Jiafeng , Wang Yixin , Tong Weiwei , Wang Mingshan , Wu Jia , Zhu Junpeng , Yan Rong , Ji Yemin , Chen Legao , Zhu Di , Wang Huafang , Chen Sheng , Liu Renyang , Lin Jingyang , Zhang Jun , Huang Haijun , Zhao Yan , Ge Minghua 
Published: May 2021

Background: The COVID-19 global pandemic has posed unprecedented challenges to health care systems all over the world. The speed of the viral spread results in a tsunami of patients, which begs for a reliable screening tool using readily available data to predict disease progression.Methods: Multicenter retrospective cohort study was performed to develop and validate a triage model. Patient demographic and non-laboratory clinical data were recorded. Using only the data from Zhongnan Hospital, step-wise multivariable logistic regression was performed, and a prognostic nomogram was constructed based on the independent variables identifies. The discrimination and calibration of the model were validated. External independent validation was performed to further address the utility of this model using data from Jinyintan Hospital.Results: A total of 716 confirmed COVID-19 cases from Zhongnan Hospital were included for model construction. Men, increased age, fever, hypertension, cardio-cerebrovascular disease, dyspnea, cough, and myalgia are independent risk factors for disease progression. External independent validation was carried out in a cohort with 201 cases from Jinyintan Hospital. The area under the curve (AUC) was 0.787 (95% confidence interval [CI]: 0.747–0.827) in the training group and 0.704 (95% CI: 0.632–0.777) in the validation group.Conclusions: We developed a novel triage model based on basic and clinical data. Our model could be used as a pragmatic screening aid to allow for cost efficient screening to be carried out such as over the phone, which may reduce disease propagation through limiting unnecessary contact. This may help allocation of limited medical resources.
Safety, Tolerability, and Proof-Of-Concept Study of OKV-119, a Novel Exenatide Long-Term Drug Delivery System, in Healthy Cats
Authors: Klotsman Michael , Adin Christopher A. , Anderson Wayne H. , Gilor Chen 
Published: May 2021

Background:Glucagon-like peptide-1 (GLP-1) is an incretin hormone that plays an important role in glucose homeostasis and food intake. In people, GLP-1 receptor agonists (GLP-1RAs) are commonly used for the treatment of type 2 diabetes mellitus (DM) and obesity; however, non-adherence to injectable medications is common. OKV-119 is an investigational drug delivery system intended for subdermal implantation and delivery of the GLP-1RA exenatide for up to 6 months.Hypothesis/Objectives:Develop protocols for the subcutaneous (SC) insertion and removal of OKV-119 and to evaluate its tolerability,in vivodrug-releasing characteristics, and weight-loss effects in cats.Animals:Two cadaveric and 19 purpose-bred cats.Methods:In cadavers, OKV-119 insertion protocol and imaging were performed at three SC locations. The safety and tolerability of OKV-119 implants were assessed in a small (n= 4 cats) 62-day study. Weekly plasma exenatide concentrations and body weight were measured in a 42-day proof-of-concept study designed to evaluate OKV-119 prototypes implanted in cats (n= 15).Results:In anesthetized cats, the duration of insertion and removal procedures was 1–2 min. OKV-119 was easily identified on radiographs, and well-tolerated without any apparent implant site reactions. Following implantation, exanatide plasma concentrations were observed for up to 35 days. Plasma exenatide concentrations were correlated to weight loss.Conclusion and clinical importance:Our findings suggest that OKV-119 could be easily inserted and removed during a routine clinic visit and can be used to safely and effectively deliver exenatide. Future studies of OKV-119, configured to release exenatide for a longer extended months-long duration, are warranted to determine whether the combination of metabolic improvements and beneficial weight-loss, coupled with minimal impact on pet-owner's lifestyle, lead to improved outcomes for obese cats and feline DM patients.
Patient risk factor stratification is essential for the hospital antibiogram
Authors: Tiwari Karuna , Patil Samruddhi , Naik Aparna , Shetty Anjali , Walia Kamini , Rodrigues Camilla 
Published: May 2021

Empiric antimicrobial therapy in hospitalized patients is guided by an institution’s cumulative antibiogram, which may not be adequate in giving information on decision-making for optimal treatment in different patient populations. Adding patient risk factors can make it more useful for clinicians in guiding empiric therapy and for antimicrobial stewardship. Cumulative data were obtained for blood culture and urine isolates from the laboratory information system of a tertiary care hospital for 6 months (January to June 2019). Further stratification of organism types and resistance rates on the basis of patient risk factors (Patient Types 1, 2, and 3) was performed and analyzed. Salmonella spp. was seen in community-acquired ward patients (Types 1 and 2). Streptococcus pneumoniae was seen in Type 1 patients, and Acinetobacter spp. was seen in Type 3 patients. Extended-spectrum beta-lactamase-producing gram-negative infection rates were higher in community patients than in hospital patients. Carbapenem-resistant Enterobacteriaceae rates were high in Type 3 hospitalized patients. Cumulative blood methicillin-resistant Staphylococcus aureus rates were 43% but stratification showed it only in Type 2 and Type 3 ICU patients with 0% in ward patients. Stratified antibiograms based on patient risk factors are valuable for antimicrobial stewardship and help to optimize empiric therapy and increase the understanding of antimicrobial resistance trends.
Spectral Characterization of Macro-Heterocyclic Compound RhTMPyP / ZnTSPc
Authors: OLTEANU Liviu , ION Rodica Mariana , TEODORESCU Sofia , ŞTIRBESCU Raluca Maria 
Published: May 2021

Macro-heterocyclic compounds, such as porphyrins and phthalocyanines, are being studied extensively for their important physical and chemical properties. Their ability to absorb light throughout the spectrum and self-organization being adequate for the realization of several applications. Investigations of the spectral properties of the supramolecular assembly RhTMPyP/ ZnTSPc- 5,10,15,20 rhodium tetramethyl-pyridyl porphyrin / 2,9,16,23 zinc tetrasulfonated-phthalocyanine are registered by UV-Vis, FT-IR and Raman spectroscopic techniques.
PENENTUAN DOSIS FORMULASI PUPUK ORGANIK UNTUK EFISIENSI PENGGUNAAN PHONSKA PADA TANAMAN JAGUNG MANIS (Zea mays sacharata Sturt.)
Authors: Tulungen A. G. , Tumewu P. , Montolalu M. , Rantung John L. , Tulung S. 
Published: May 2021

The use of organic fertilizer provides soil with macronutrients, micronutrients, and organic acids which improve the long-term physical, chemical, and biological fertility of the soil. This research aims to: 1) Increase physical and chemical soil fertility, 2) Acquire the interaction between organic fertilizer and Ponska fertilizer dosage on sweet corn crops, 3) Acquire the most efficient dosage of PONSKA fertilizer on sweet corn, and 4) disseminate research results through journal articles and directly to farmers. This research will increase the efficiency of NPK Ponska chemical fertilizers by utilizing waste from around the farm and lakes to formulate organic fertilizer to apply on sweet corn crops and as a reference to teaching material. Factorial research with two factors was designed using a randomized block design. Factor I (A) = Organic fertilizer formulation, comprising of A1 = 20 ton/ha, A2 = 30 ton/ha, and A3 = 40 ton/ha. Factor II (B) = Phonska fertilizer, comprising of B1 = 25 % Ponska, and B2 = 50% Ponska. Every treatment was replicated four times, yielding 24 research plots. Response variables observed were: Plant height, number of leaves, dry weight of the plant. Data were analyzed using ANOVA, followed by Tukey's honest significance difference test (alpha = 0.05) if significant differences were found. Results showed that the interaction between organic and Phonska fertilizer formulations had significant effects on plant height. A dosage of 40 ton/ha of organic fertilizer can decrease the use of phonska fertilizer by 75% based on the height of sweet corn plants.
PENGARUH PENAMBAHAN TEPUNG TAPIOKA DALAM PEMBUATAN BISKUIT BERBAHAN BAKU TEPUNG UBI BANGGAI (Dioscorea alata L.)
Authors: Sofyani Selvy , Kandou Jenny E. A. , Sumual Maria Fransisca 
Published: May 2021

ABSTRACTThe purpose of this study was to determine the proportion of tapioca flour that needs to be added in the manufacture of biscuits made from Banggai yam flour, based on sensory and chemical properties. This research used a Completely Randomized Design (CRD) with the addition of tapioca flour at four levels as treatments, i.e. A: 0%; B: 5%; C: 10%; D: 15%, which was triplicated. The parameters observed were organoleptic testing for the degree of preference for taste, aroma, color and texture. The chemical composition of the biscuits was analyzed and the energy value was calculated. Overall, the level of preference given by the panelists on the Banggai yam biscuits without the addition of tapioca flour was 3.68 (like) out of 5 as the highest value. It can be concluded that tapioca flour does not need to be added in making Banggai yam biscuits. Banggai yam biscuits have a hardness of 1.93 mm / g / sec. and energy value of 422.62 kkal, with the composition of 3.98% moisture, 8.13% protein, 11.10% fat, 3.68% ash, 72.55% carbohydrates, and 0.56%. crude fiber. Keywords: Banggai yam, tapioca flour, biscuits
PENGARUH PENAMBAHAN SARI BUAH SIRSAK TERHADAP SIFAT SENSORIS MINUMAN ISOTONIK AIR KELAPA (Cocos nucifera L.)
Authors: Lempoy Wulan Kristie , Mandey Lucia Cecilia , Kandou Jenny E. A. 
Published: May 2021

ABSTRACTThis study aims to determine the proportion addition of soursop juice and analyze the chemical contents of isotonic drink from mature coconut water (Cocos nucifera L.) This research was conducted by adding 100 ml, 200 ml, and 300 ml of soursop juice to 1000 ml of coconut water. The test parameters were organoleptic tests using hedonic methods including flavor, taste, and turbidity. The best treatment was followed by chemical testing including sodium, potassium, acidity (pH), total acid and total sugar. The result of organoleptic test showed that 1000 ml of coconut water without soursop juice addition has a value of 2,73 (neutral), while the addition of 100 ml soursop juice has a value of 3,43 (neutral), the addition of 200 ml soursop juice has a value of 3,54 (like), and the addition of 300 ml soursoup juice has a value of 3,71 (like). In conclusion, the best result is the addition of 300 ml soursop that had a mineral content of sodium as much as 469,36 mg/kg, potassium as much as 2014,45 mg/kg, pH 4,65, total acid 0,54% and total sugar 6,33%. Keywords: Isotonic Drinks, Soursop Juice, Coconut Water
KAJIAN IRIGASI MIKRO PADA SISTEM HIDROPONIK PADI (ORIZA SATIVA L.) VARIETAS SERAYU DALAM RUMAH TANAMAN
Authors: Moningka Christian N. G. , Ludong Daniel P. M. , Rumambi David P. 
Published: May 2021

ABSTRACTThe purpose of this study was to examine the effect of the thickness of irrigation water flow in the Deep Flow Technique (DFT) hydroponic system on the amount of plant water requirement (or evapotranspiration) and the Water Use Efficiency (WUE) in rice cv. Serayu at the vegetative phase. This research was carried out in a tropical greenhouse roofed with polyethylene (PE) plastic film. The treatments in this study were three kinds of thickness of micro irrigation in the DFT hydroponic system, i.e. 2, 4 and 6 cm. The results showed that water requirement for each planting hole in rice plants during the vegetative phase (60 days) were 14.3650, 12.7025, and 11.3108 with biomass of 62.4576, 58.2874 and 52.6370 g, respectively for 6, 4 and 2 cm. The water use efficiency in 2, 4, 6-cm-DFT-system were 2.3268, 2.293 and 2.1740 g/L, respectively. Keywords: Hidroponics, Deep Flow Technique, Evapotranspiration, Water Use Efficiency
ANALISIS KUALITAS SELADA (Lactuca sativa L.) YANG DITANAM PADA DUA MEDIA SELAMA PENYIMPANAN DINGIN
Authors: Rantung Livia E. , Lengkey Lady C. Ch. E. , Wenur Frans 
Published: May 2021

ABSTRACTThe objective of this study was to investigate the quality (weight, color, stem firmness and total dissolved solids) of hydroponic and soil culture lettuce (Lactuca sativa L.). This study used a non factorial completely randomized design with two treatments (type of planting media) as follows hydroponic lettuce and soil culture lettuce. This research conducted 9 replications. Data were analyze with ANOVA to determine whether there is a difference in the quality of lettuce after storage at cold temperatures. Lettuce is packed and placed at cold storage of 50C. The change in weight, color, stem firmness and total dissolved solids were observed every 3 days during storage period. The result of this study show that hydroponic lettuce and soil lettuce gave different responses during cold storage. After 15 days of storage, soil culture lettuce has better quality than hydroponic lettuce in terms of maintaning the weight and total dissolved solids. While, in maintaning the color and firmness of the stem, hydroponic lettuce has better quality. Keywords: Lettuce, planting media, cold storage
UJI KINERJA ALAT PENYULINGAN NIRA AREN MENJADI BIOETANOL DI KELOMPOK TANI HUTAN TAYAPU DESA TALAWAAN KECAMATAN TALAWAAN KABUPATEN MINAHASA UTARA
Authors: Solar Trivano , Wenur Frans 
Published: May 2021

ABSTRACTThis study aims to determine the performance of the distillation tool aid by the North Sulawesi Forestry Service for the Hutan Tayapu Farmer Group consisting of temperature conditions in the distillation process, alcohol content, distillation rate, product yield, and energy consumption as well as to calculate the energy efficiency of the distillation tool.This research uses experimental methods. Data is presented in tables and graphs, then computed and analyzed descriptively. Bioethanol production is carried out 2 times each using 100 liters of palm sap. The results showed that the distillation process took place at a temperature ranging from 29.2 °C to 100.9 °C. The obtained alcohol content ranged from 26% to 64%, with energy efficiency of 4.96% for the appliance, and the distillation rate in the range of 1.87-8.40 liter/hour. Key words: palm sap, bioethanol, performance evaluation, energy efficienc
KAJIAN MUTU LABU SIAM (Sechium edule) TEROLAH MINIMAL YANG DIKEMAS VAKUM SELAMA PENYIMPANAN
Authors: Pinem Esron H. , Longdong Ireine A. , Lengkey Lady C. Ch. E. 
Published: May 2021

AbstractThis study aims to analyze the deterioration of the quality of the chayote during storage using several packaging methods. The research method was carried out experimentally and the data were processed descriptively which consisted of three treatments, namely: vacuum packed, without vacuum, and open. Each treatment was carried out 3 (three) times with each weight of 250 grams. Research shows that minimally processed chayote which is vacuum packed and stored at a temperature range of 5oC - 9.6oC can last for 12 days, experiencing a weight loss of 0.90%. The color changed from Faded Yellow to Gray Yellow. The texture becomes soft with a hardness value of 8.80 N. The water content changes from 91.98% to 94.70%.In conclusion, the storage life of vacuum packed minimally processed chayote could be prolonged up to 12 days at 5oC – 9,6oC, grey-yellow in color, texture softened, and weight loss as much as 0.90%. Keywords: Chayote, minimally processed, vacuum packed.
Indirect-to-direct band gap crossover of single walled MoS2 nanotubes
Authors: Hisama Kaoru , Maruyama Mina , Chiashi Shohei , Maruyama Shigeo , Okada Susumu 
Published: May 2021

Abstract
Using density functional theory, the electronic structures of single walled molybdenum disulfide nanotubes (MoS2 NTs) were investigated. The armchair MoS2 NTs are indirect gap semiconductors for diameters up to approximately 5.2 nm, while those with larger diameters are direct gap semiconductors with band edges located in the vicinity of k = 2π/3. This finding implies that MoS2 NTs with large diameters should exhibit similar photoluminescence to 2D monolayer MoS2 sheets. This indirect-to-direct band gap crossover accounts for the relative upward shift of the valence band peak at the Γ point in small diameter NTs, owing to the tensile strain arising from curvature.
O trzech zabytkach muzyczno-liturgicznych z kolekcji Baworowskich przechowywanych w Bibliotece Narodowej
Authors: Chachulska Irina 
Published: May 2021

Badania nad trzema muzyczno-liturgicznymi źródłami pochodzącymi z kolekcji Baworowskich, a przechowywanymi obecnie w Bibliotece Narodowej – graduałem PL-Wn 12496 IV oraz fragmentami PL-Wn 12497 IV i PL-Wn 12498 IV – zarówno weryfikują dotychczasowy stan wiedzy na ich temat jak też rozjaśniają wątpliwości i sprzeczności wynikające z najstarszych opisów rękopisów. Trzy różnorodne pod względem paleograficznym źródła, których zapisy pochodzą z odległych od siebie czasów, okazały się tworzyć niegdyś jedną księgę. Oba fragmenty w całości stanowią późniejsze dodatki do graduału, które odłączono od zasadniczej części najprawdopodobniej w I poł. XIX wieku. Graduał – a właściwie jego zasadnicza, oryginalna część – powstał w skryptorium lubiąskim ok. 1250 r. dla sióstr cysterek w Trzebnicy, bardzo możliwe iż był darem dla córki św. Jadwigi – Gertrudy. Wskutek sekularyzacji klasztorów na Śląsku (1810 r.) rękopis najprawdopodobniej został wywieziony z Trzebnicy do Wrocławia, jednak ostatecznie nie zasilił zbiory Biblioteki Uniwersyteckiej, tak jak zasadnicza część biblioteki klasztornej, lecz znalazł się w prywatnym posiadaniu wrocławskiego muzyka J.Th. Mosewiusza. Po jego śmierci rękopis, już z odseparowanymi fragmentami, wzbogacił kolekcję Wiktora Baworowskiego we Lwowie, gdzie trzy części księgi otrzymały odrębne sygnatury. Po wojnie (1945 r.), już jako trzy nie związane ze sobą źródła – poza ich lwowską proweniencją – graduał z fragmentami trafił do zbiorów Biblioteki Narodowej. W świetle przeprowadzonych badań rękopis, choć powstały w skryptorium lubiąskim, wolno nazwać „Graduałem Trzebnickim”. Księga należy do grupy najdawniejszych zabytków muzyczno-liturgicznych przechowywanych w Polsce i dokumentuje najstarszy zachowany przekaz wersetu allelujatycznego O felix Hedwigis (PL-Wn 12496, f. 1r) jak też jeden z najstarszych zapisów sekwencji o św. Jadwidze Consurge jubilans (PL-Wn 12498).