bluuebunny/crossref_metadata_2025_split huggingface dataset:
Development of scattered radiation distribution visualization system using WebAR
Authors: Nishi K , Fujibuchi T , Yoshinaga T 
Published: July 2021

Abstract
Radiation protection education is difficult for some radiological workers to taking because they are busy with medical work. In radiation protection, understanding the behaviour of scattered radiation is important for reducing exposure. Although applications that visualize the behaviour of scattered radiation using augmented reality or virtual reality have been developed, such applications are limited by the need to download the application and the performance of the device. We have developed a system that can be used in a web browser to visualize the behaviour of scattered radiation more easily. Monte Carlo method was used to simulate the behaviour of scattered radiation during radiography using a portable X-ray machine. An augmented reality (AR) system was developed using A-Frame, an open-source web framework, and AR.js, which adds the AR function. Finally, the behaviour of scattered radiation was observed using various devices. With AR, the behaviour of scattered radiation was visualized in three dimensions. The newly developed AR system can be used with web browsers to easily learn the behaviour of scattered rays without the need for special devices.
Basic study on evaluation of X-ray dose distribution using plastic scintillator plate and digital CMOS camera
Authors: Yoshitani H , Fujibuchi T , Anam C 
Published: July 2021

Abstract
Evaluation of dose distribution in a phantom is effective for quality control of radiation diagnostic equipment and evaluation of exposure. However, measurements at multiple points using a dosimeter is complicated. An alternative is to combine a plastic scintillator (PS) plate and digital camera to obtain the dose distribution of the entire phantom. In this study, the basic characteristics of this system were examined. A PS was sandwiched between Polymethyl Methacrylate (PMMA) disk phantoms, and irradiation was performed while the tube current was changed from one direction. The tube voltage was set to two values, specifically 60 and 120 kV. During irradiation, the entire phantom was recorded at 0.1 s intervals using a digital Complementary Metal Oxide Semiconductor (CMOS) camera. The lowest dose rate where luminescence could be detected was approximately 1 mGy per 0.1 s. The amount of luminescence increased linearly with the dose rate. The proportional relationship that was confirmed between dose rate and luminescence suggests the possibility of using the system for dose estimation. This proposed dose-evaluation method can enable the dose distribution to be evaluated with a time resolution of 0.1 s and a high spatial resolution.
Molecular identification of inulinolytic yeast isolated from cherry fruit (Muntingia calabura L.) based on internal transcribed spacer sequence
Authors: Erfianti T , Wijanarka W , Kusumaningrum H P 
Published: July 2021

Abstract
Inulinolytic yeast is a yeast that produces inulinase enzyme (E.C.3.2.1.7). Inulinase has an important role to hydrolyze inulin into simpler molecules that are widely used in industry. Previous research has obtained K4 inulinolytic yeast from cherry fruit (Muntingia calabura L.) which has not been identified yet. This study aims to molecularly identify and phylogenetic analysis of K4 inulinolytic yeast isolated from cherry fruit (M. calabura L.) based on the Internal Transcribed Spacer (ITS) sequence. Research stages consist of DNA isolation, amplification of the ITS rDNA sequence, electrophoresis of PCR product, analysis of sequence data, and phylogenetic analysis using MEGA X. The results showed that yeast DNA was successfully obtained with a concentration of 807.1 ng/µL and had a purity value of 2.12. Amplification of the ITS rDNA sequence produced amplicon with length ±250 bp. Molecular identification based on homology analysis with database of The National Center for Biotechnology Information (NCBI) and phylogenetic analysis indicated that K4 isolate was the most closely related to Diutina rugosa isolate S217 (MF797783).
Isolation, antibacterial activity, and molecular identification of endophytic fungi from Pogostemon cablin
Authors: Pratama N A , Widyarifa M M , Farikha S L , Kusumaningrum H P , Eshananda Y 
Published: July 2021

AbstractPogostemon cablinis the medicinal plants that produces patchouli essential oils as secondary metabolites which has multiple functions including antibacterial ability. The secondary metabolites in plants mostly associated with their endophytic fungi. In this study we isolated endophytic fungi fromPogostemon cablin’s leaves and examined antibacterial activity of the endophytic fungi againstEscherichia coliandStaphylococcus aureus,as well as find out the identity of most potential isolate based on Internal Transcribe Spacer (ITS) region. The isolation of endophytic fungi was performed using surface sterilization method on Malt Extract Agar (MEA) medium. The antibacterial activity was tested using paper disc on Muller Hinton Agar (MHA) medium and molecular identification was amplified using ITS 4 and ITS 5 primers. The isolation process resulted in 5 isolates of endophytic fungi. The antibacterial assay indicated one potential isolate with the highest antibacterial activity when tested againstE. coliandS. Aureus,exhibited 20.9 mm and 19 mm clear zone respectively. Molecular identification from ITS region database depicted that the potential isolate has high homology withNigrosporasp. by 99% similarity. This result suggested that the antibacterial ability of essential oils from thePogostemon cablin’s leaves might has high correlation with the occurrence of endophytic fungi.
Chiroptera population as bioindicators of urbanization and fragmented habitat in West Java
Authors: Basukriadi A , Nurdin E , Adiwibowo A , Kamilia N I , Jason P , Roebert P , Ulibasa B G , Suryaatmaja T N S , Tobing R H G 
Published: July 2021

Abstract
One of the threats to the natural ecosystem is urbanization. The deforestation and development of settlements have caused fragmented natural habitat, vegetation cover reduction, and decline of biodiversity. In urban, one of the mammalian groups that can adapt to the presence of settlement is the Chiroptera group or commonly known as bats. One of the areas that have experienced rapid urbanization was located in West Java. Considering this condition, this study aims to study the impact of urbanization and the potential use of Chiroptera as an urbanization bioindicator. The method used was line transect across the landscape mosaic located in 2 urbanized areas in West Java, i.e., Bekasi and Depok. There was 3 line transects in each area. Three locations were selected from each transect and sampled with 3 replications. The density of Chiroptera and the percentage of vegetation covers were observed along transects. In total, there were 18 observations. The study confirms the presence of fragmented landscape with vegetation cover mean ranged from 39.8%/100 m2 (95% CI: 20.4% to 59.2%). to 65.3%/100 m2 (95% CI: 52.5% to 78.1%). While the density of Chiroptera mean ranges were 2.18 inds. /100 m2 (95% CI: 1.44 to 2.92)-2.85 inds./100 m2 (95% CI: 0.98 to 4.72). There was a significant positive correlation (p = 0.004, r= 0.630) between vegetation cover and Chiroptera abundance. Based on the correlation, the less fragmented landscape was indicated by high Chiroptera abundance.
Abundance and composition of phytoplankton communities in middle stream of Citanduy river, West Java
Authors: Sawestri S , Rais A H 
Published: July 2021

Abstract
The relationship between phytoplankton composition and environmental conditions in their habitat provides an important role for their as an assessment of the status and quality of river waters and the management of these waters. The aim of this research was studied of composition and abundance of phytoplankton communities in Citanduy River, particularly in middle stream. The study has conducted in April, July, and October 2018. The study location consists of 4 stations. The research has measured the abundance index, diversity index, and dominance index of plankton. The physic-chemical waters parameters which was measured consist of temperature, pH, DO, depth, transparency, nitrite, ammoniac, nitrate, total phosphate, and chlorophyll-a. Phytoplankton of Citanduy River consists of 4 classes. The abundance index of phytoplankton was around 2,578-7,366 cells/L; diversity index of phytoplankton is around 0.48-2.57, while the highest dominance index was Pediastrum sp with 0.56. Based on multiple regression analysis, the abundance of phytoplankton in April was influenced by nitrates and temperature. The abundance of phytoplankton in July is influenced by nitrite and ammonia. Meanwhile, in October, phosphate and oxygen parameters affect the abundance of phytoplankton. The Citanduy River is still in the stable category because the water quality is still in a sufficient condition for aquatic biota, especially phytoplankton. The ammonium and total phosphate of BBWS station in October were higher than standard. The high concentration of nitrogen and phosphorus nutrients is the results from the surrounding land for rice fields, plantations, fish farming, and waste disposal.
Formulation and optimization of alternative culture media for probiotic bacteria growth using tofu liquid waste
Authors: Jannah S N , Pujiyanto S , Rosiana E , Purwantisari S 
Published: July 2021

Abstract
Probiotics from lactic acid bacteria (LAB) are widely used in the livestock industry to replace the use of Antibiotics Growth Promoters (AGPs). LAB is a culture starter that is widely used in fermented foods. LAB has the ability to adapt to different substrates, resulting in its wide use in the fermentation of various food products. MRS (De Man Rogosa Sharpe) medium is a growth medium for LAB, but for the industrial-scale requires a high cost, therefore innovation is needed to find alternative culture media using local raw material. Tofu Liquid Waste (TLW) can be used as a growth medium for probiotic bacteria because it still contains enough nutrients source for growth. This study aims to determine the formulation and optimization of culture media for LAB isolates using tofu liquid waste. Six Lab isolates from chickens gastrointestinal tract were used in experiments to determine the ability to grow on TLW culture media. The growth assays are conducted by inoculating LAB isolates in the culture media formulation that divided into 4 groups, consisting of medium A (TLW+molasses 1,5%+skim milk 5%), B (TLW+molasses 1,5%+skim milk 2,5%), C (TLW+molasses 3%+skim milk 5%), and D (TLW+molasses 3%+skim milk 2,5%). The best LAB isolate to grow on TLW medium was Lactobacillus paracasei. The best TLW modification medium for Lactobacillus paracasei growth is D media. Modified media consisting of TLW, molasses and skim milk can be used as an alternative medium for LAB growth.
Characteristics of liquid organic fertilizer from different composition types of seaweed between Gracilaria sp. and Sargassum sp.
Authors: Tsaniya A R , Dewi E N , Anggo A D 
Published: July 2021

Abstract

Gracilaria sp. and Sargassum sp. contain growth hormones such as auxins, gibberellins, and cytokinins. A combination of them is expected to complement each other as raw material for organic fertilizers to improve soil structure and help plant growth. The purpose of this study is to determine the characteristics of organic fertilizer from different seaweed composition. Reduction the size of sample and soaking with 1% phosphoric acid solution to soften the seaweed. Homogenization and heating with the addition of molasses 3% as microbial nutrition and KOH 1% to maximalize mineral content. The fermentation process is carried out for 14 days as microbial exponential phase of Azospirilum sp as nitrogen fixation also phytohormones producer and Trichoderma sp. as an organic material decomposer. The results of ANOVA analysis and Tukey test showed that the treatment of raw material composition using different types of seaweed gives significantly different in C-Organic, nitrogen, potassium, phosphorus, pH, color, and aroma. The composition of Gracilaria sp. and Sargassum sp. give the highest value for C-Organic (1.15%) and Nitrogen (0.67), as well as phosphorus (0.45%), potassium (0.48%), and pH (4.48). Color and aroma are maturity indicators of organic fertilizers. This composition of liquid fertilizer has a pungent aroma and a dark brown color.
Does moringa oleifera leaf nanoparticles increase albumin levels in stunting toddlers?
Authors: Gustiya S D , Hadisaputro S , Sumarni S 
Published: July 2021

Abstract
Stunting is a delay in growth that in children under 5 years. The incidence of stunting around 22% in the world in 2017. Macro nutrients such as carbohydrates, proteins and fats affect growth in toddlers. The cause of decreased albumin levels in blood plasma occurs due to low protein intake and inadequate protein absorption. The aim of the study is to identify the effect of Moringa Oleifera leaf nanoparticles in albumin levels. This study was a quasi experimental with pretest and posttest control group design. Twenty toddlers in the treatment group was given Moringa Oleifera leaf nanoparticles with a dose of 65 mg/day and supplementary feeding. While twenthy stunting toddlers in the control group comsumed supplementary feeding only. The interventions was given for 21 days. The instrument used to measure albumin levels is Bromcresol Green (BCG). Data analysis used paired T Test and Mann Whitney. Most toddlers are female at the intervention group. On the other hand, more fale at control group. The intervention group was younger than in the control group (28 and 37 months, respectively). Moringa Oleifera leaf nanoparticles increased significantly albumin levels 0,278 g/dL (p=0,001). While in the control group albumin levels increased slightly 0,028 g/dL (p=0,150). Moringa Oleifera leaf nanoparticles proved to improve albumin levels greater than the control group in stunting toddlers.
Seed variation of Rubus spp. in Cibodas Botanical Garden
Authors: Surya M I , Destri D , Ismaini L 
Published: July 2021

Abstract
The genus Rubus has highly morphological diversity. We had examined the seed variation of Rubus spp. collected in Cibodas Botanical Garden. The observation was conducted on 12 different species of Cibodas Botanical Garden’s collections. The variation of seed’s size was observed based on length, width, and sculpture of seed’s surface area. The results showed that R. pyrifolius seed was larger among others species, and the smaller one was R. fraxinifolius. Moreover, based on seed morphology, three morphological types of seed coats were identified, which are seeds with prominent reticulate sculpture and sharp back ridge (R. accuminatissimus, R. alpestris, R. ellipticus, R. lineatus, R. niveus, R. pyrifolius, R. rosifolius); seeds with prominent reticulate sculpture and broad back ridge (R. alceifolius, R. moluccanus, R. glomeratus); seeds with least prominent sculpture and blunt back ridge (R. chrysophyllus and R. fraxinifolius).
Use of periodically hCG hormones injection for the gonadal development of java barb (Puntius javanicus) as bioreproduction applied on aquaculture
Authors: Nugroho R A , Yuniarti T , Basuki F , Hastuti S , Listiarini  
Published: July 2021

AbstractTawes fish or Java barb (PuntiusjavanicusBleeker, 1855) as the one of the endogenous species in Indonesia has specific reproductive roles that its cycle still depends on local environment. It makes limited frequency of spawning in nature that has been decreasing seeds production either domestication or cultivation. As a seasonal spawner, it needs long time for gonadal development. Gonadal development of endogenous species broodstock by hormone is needed to improve bio-reproductive capacity and it will cause faster gonadal maturity of Java Barb. One of exogenous hormones that could trigger gonadotropin hormone (GtH) is the human chorionic gonadotropin (hCG) hormone and it will accelerate the maturity level of the gonad by periodically injection. The aim of research is to determine the effect and the best dosage of hCG hormone for the gonadal development of Java barb. This study used an experimental method with a completely randomized design, with 4 treatments and 3 repetitions in 30 days observed. 48 broodstock with 200 gram average weight were used and induced by hCG. The doses of hCG are 0, 100, 200 and 300 IU/kg. The parameters observed include the level of gonad maturity, gonado somatic index (GSI) and hepatosomatic index (HSI). After statistical analysis, result showed that dose of 200 IU could improve the best development of gonad in Java barb base on 3rd maturity level achieved, gonado somatic index (10.11 ± 0.74%) and the hepatosomatic index at (1.99 ± 0.06%) respectively. The observation which used hCG hormone can improve gonadal development and one step closer to reproduce domesticated and cultived of Java Barb.
DNA isolation of clove and lemongrass using modification of the Doyle and Doyle methods and their relation with antioxidant activity
Authors: Listyanto H A , Muhammad I M , Erfianti T , Herida A P , Kusumaningrum H P , Loka B D , Setyowati E 
Published: July 2021

Abstract
Clove (Syzigium aromaticum) and lemongrass (Cymbopogon winterianus) are essential oil-producing plants that are widely available in Indonesia and have high economic value for medicinal and industrial needs because of their antioxidant activity. However, the information about the relationship between antioxidant activity and the DNA content in these plants was very limited. Especially because DNA isolation of aromatic plants is also not easy to do with methods that are widely used. Therefore, this study aims to isolate DNA from clove and lemongrass using a modified Doyle and Doyle method and to find the relationship between the quality and quantity of DNA with their antioxidant activity. The method used was the use of PVP, isopropanol and sodium acetate for the modification of the Doyle and Doyle method in DNA isolation followed by antioxidant analysis using DPPH. The results showed that the modification to the Doyle and Doyle method had obtained high concentrations of DNA. Besides that, the clove DNA content is higher than lemongrass which is supported by the antioxidant activity of both plants.
The performance of survival and immunity of catfish (Clarias gariepinus) juvenile infected by Aeromonas hydrophila after treated by prebiotic extract in the diet
Authors: Prayitno S B , Riyadi S , Sarjito  , Desrina  
Published: July 2021

Abstract
Herb/plant can act as a prebiotic. Red ginger has long known as an anti-inflamation and anti-oxidant. Similarly, moringa leaf is also known as herbal antibiotic. In the recent years, the use of natural ingredients to stimulate immune system and to combat fish disease in aquaculture becoming a favourable choice. The aims of this research was to observe the red ginger and moringa leaf extractin the diet to the performance of catfish infected by Aeromonas hydrophila. Experimental laboratory using a completely randomized design with 6 treatments and 3 replication used in this study. The treatments were mixture of (A) 500 ppm and 0 ppm, (B) 375 ppm and 150 ppm, (C) 250 ppm and 300 ppm, (D) 125 ppm and 450 ppm, (E) 0 ppm and 600 ppm, (F) 0 ppm and 0 ppm mixture of red ginger and moringa leaf extract respectively. One hundred and eighty catfish juvenile were reared in 18 aquarium. They were fed with various concentration of prebiotic mixture for 14 days. They were infected with 1 mL A. hydrophila at 1.2 105cell/cc intramuscularly and observed for 10 days for their survival and immune response. Results showed that addition of red ginger and moringa leaf extract significantly improve the immunity of catfish. The addition of moringa leaf extract at 600 ppm was significantly improved survival rate, phagocytosis activities, leucocyte, lymphocytes count compared to other prebiotic mixtures. Thus, the usage of 600 ppm moringa leaf extract in the diet provided the best performance to catfish.
Molecular genetics and epigenetics of temporomandibular disorder
Authors: Antolis M , Langit K S , Gultom F P , Auerkari E I 
Published: July 2021

Abstract
Temporomandibular joint (TMJ) has an important role in stomatognathic system. Its role during function is facilitated from rotation and translation movement. Any deviation from TMJ normal anatomy and movement could lead into either clicking, crepitus, or pain in preauricular area. These sign and symptoms, which are widely referred as TMJ Temporomandibular joint disorder (TMD), extremely common in world population. Several genes have been identified contribute in susceptibility towards TMD. Genetic polymorphism are a form of gene sequences variance that is found in more than 1% of world population. Epigenetics is an interaction between internal and external environments that leads to a change in chromatin structures that switches the gene expression on and off. There are several factors that posibly affect the genetic polymorphisms in TMD such as; serotonin, cathecolamine, estrogen, folate, human leukocyte antigen (HLA), extracellular matrix, transcription factors, transforming growth factor beta (TGFβ), epithelial growth factor, β-catenin, and discoidin. Epigenetic mechanisms such as DNA methylation, histone modification, and microRNA are found in chondrocyte of TMD patients. In a temporomandibular joint, miRNA-140 controls bone homeostasis especially on the articular remodeling. Genetic molecular and epigenetic study will benefit in diagnosis and treatment of TMD patient. The aim of this paper is author want to inform about molecular genetics and epigenetics of TMD.
Distribution of wingless type 3a (Wnt3a) Rs 752107 gene polymorphism on orofacial cleft patients
Authors: Simbolon U T H , Alima I R N , Kim K R , Yuniastuti M , Suhartono A W , Auerkari E I 
Published: July 2021

Abstract
Non-syndromic orofacial cleft, including cleft lip with or without cleft on palate (CB +/- CL NS) is the most common disorder resulting from craniofacial development disorder during pregnancy. These developmental abnormalities occur due to interference during the fusion stage or time and position disturbance of the processes and/or palatal shelves. The prevalence of orofacial cleft is around 1:500 - 1:2000 in the world. Wnt3a, which is the genes belonging to the Wnt gene family controls craniofacial development during pregnancy. It involves regulating the development of the middle face and upper lip fusion. Therefore this gene plays an important role as a cause in non-syndromic cleft lip/palate. The relationship of Wnt3a gene polymorphism to the orofacial cleft occurrence in Indonesia remains unclear. To examine the relationship of polymorphism of Wnt3a Rs 752107 genes in orofacial cleft patients in Indonesia. The study samples are raw material stored in the Oral Biology Laboratory of the Faculty of Dentistry, the University of Indonesia in the form of DNA from cleft lip patients. Study on the distribution of genetic variation of wnt3a rs 752107 in 30 samples of CB +/- CL NS patients and 170 control samples without orofacial cleft using Polymerase Chain Reaction (PCR-RFLP) examination with Alu1 enzyme. Significance of variation difference test using Chi-square test on SPSS 22. Frequency of CC were dominant in both samples (>90%). There was no significant difference between gene polymorphisms of wnt3a rs 752107 in orofacial cleft with control (p>0.05)