bluuebunny/crossref_metadata_2025_split huggingface dataset:
Phenotypic and Transcriptional Plasticity Directed by a Yeast Mitogen-Activated Protein Kinase Network
Authors: Breitkreutz Ashton , Boucher Lorrie , Breitkreutz Bobby-Joe , Sultan Mujahid , Jurisica Igor , Tyers Mike 
Published: April 2021

Abstract
The yeast pheromone/filamentous growth MAPK pathway mediates both mating and invasive-growth responses. The interface between this MAPK module and the transcriptional machinery consists of a network of two MAPKs, Fus3 and Kss1; two regulators, Rst1 and Rst2 (a.k.a. Dig1 and Dig2); and two transcription factors, Ste12 and Tec1. Of 16 possible combinations of gene deletions in FUS3, KSS1, RST1, and RST2 in the Σ1278 background, 10 display constitutive invasive growth. Rst1 was the primary negative regulator of invasive growth, while other components either attenuated or enhanced invasive growth, depending on the genetic context. Despite activation of the invasive response by lesions at the same level in the MAPK pathway, transcriptional profiles of different invasive mutant combinations did not exhibit a unified program of gene expression. The distal MAPK regulatory network is thus capable of generating phenotypically similar invasive-growth states (an attractor) from different molecular architectures (trajectories) that can functionally compensate for one another. This systems-level robustness may also account for the observed diversity of signals that trigger invasive growth.
Monosaccharide biosynthesis pathways database
Authors: Srivastava Jaya , Sunthar P , Balaji Petety V 
Published: April 2021

Abstract
A distinctive feature of glycans vis-à-vis proteins and nucleic acids is its structural complexity, which arises from the huge repertoire of monosaccharides, isomeric linkages and branching. A very large number of monosaccharides have so far been discovered in natural glycans. Experimentally, pathways for the biosynthesis have been characterized completely for 55 monosaccharides and partially for a few more. However, there is no single platform, which provides information about monosaccharide biosynthesis pathways and associated enzymes We have gathered 572 experimentally characterized enzymes of 66 biosynthesis pathways from literature and set up a first of its kind database called the Monosaccharide Biosynthesis Pathways Database http://www.bio.iitb.ac.in/mbpd/). Annotations such as the reaction catalyzed, substrate specificity, biosynthesis pathway and PubMed IDs are provided for all the enzymes in the database. Sequence homologs of the experimentally characterized enzymes found in nearly 13,000 completely sequenced genomes from Bacteria and Archaea have also been included in the database. This platform will help in the deduction of evolutionary relationships among enzymes such as aminotransferases, nucleotidyltransferases, acetyltransferases and SDR family enzymes. It can also facilitate experimental studies such as direct enzyme assays to validate putative annotations, establish structure–function relationship, expression profiling to determine the function, determine the phenotypic consequences of gene knock-out/knock-in and complementation studies.
Aging and IBD: A New Challenge for Clinicians and Researchers
Authors: Faye Adam S , Colombel Jean-Frederic 
Published: April 2021

Abstract
Evidence from recent epidemiological data suggests that the patient population with inflammatory bowel disease (IBD) is chronologically aging. As these individuals become older, cellular senescence leads to a state of chronic inflammation. This process, known as inflammaging, is thought to be closely linked with biological aging and may be upregulated within IBD. As a consequence, we see an increased risk of aging-related disorders within IBD. In addition, we see that frailty, which results from physiologic decline, is increasing in prevalence and is associated with adverse clinical outcomes in IBD. As such, in this review we explore the potential overlapping biology of IBD and aging, discuss the risk of aging-related disorders in IBD, and describe frailty and its relation to clinical outcomes within IBD. Finally, we discuss current considerations for clinical care and potential research avenues for further investigation.
Glucocorticoid-Mediated Changes in Male Green Treefrog Vocalizations Alter Attractiveness to Females
Authors: Leary Christopher J , Crocker-Buta Sarah , Holloway Ashli , Kennedy Joseph G C 
Published: April 2021

Synopsis
Adrenal glucocorticoids (GCs) are increasingly recognized as important modulators of male courtship signals, suggesting that circulating levels of these steroids can play a central role in sexual selection. However, few studies have examined whether GC-mediated effects on male sexual signals actually impact mate choice by females. Here, we examine how corticosterone (CORT)-mediated changes in the vocalizations of male green treefrogs, Dryophytes cinereus, influence attractiveness to females. In this species, agonistic acoustic signaling between rival males competing for mates increases circulating CORT levels in contest losers. Acute elevations in CORT, in turn, decrease the duration of male advertisement calls and increase the latency between successive calls, resulting in a net reduction in vocal effort (the amount of signaling per unit time) that occurs independently of changes in circulating androgens. Based on known preferences for acoustic features in D. cinereus, and other anuran species, the direction of CORT-mediated effects on temporal call characteristics is expected to compromise attractiveness to females, but whether they are of sufficient magnitude to impact female mate choice decisions is unclear. To examine whether CORT-mediated effects on male advertisement calls reduce attractiveness to females, we broadcast vocalizations in dual speaker playback experiments approximating the mean and 1 SD above and below the mean call duration and vocal effort values (the two primary vocal features impacted by elevated CORT) of males with low and high CORT levels. Results revealed strong preferences by females for the calls characteristic of males with low CORT in tests using the approximate mean and 1 SD above the mean call duration and vocal effort values, but females did not show a preference for calls of males with low CORT in trials using call values approximating 1 SD below the mean. Overall, females preferred males with signal traits predictive of low CORT, however this effect was nonlinear with attenuated preferences when signal alternatives differed only marginally indicating a possible thresholding effect. Specifically, females appeared to discriminate between males with low versus high CORT based primarily on differences in call rates associated with CORT-mediated changes in call duration and vocal effort. Our results highlight that changes in circulating CORT during male–male vocal interactions can decrease attractiveness to females, suggesting that circulating levels of CORT can play a critical role in both intra- and intersexual selection.
Does lung cancer screening with low-dose computerized tomography improve survival?
Authors: Manuel Lucy , Fong Laura S , Ly Thompson , Meredith Graham 
Published: April 2021

Summary
A best evidence topic in thoracic surgery was written according to a structured protocol. The question addressed was ‘Does lung cancer screening with low-dose computerised tomography (LDCT) improve survival?’ More than 963 papers were found, of which 8 randomized control trials and 1 meta-analysis represented the best evidence to answer the clinical question. The authors, journal, date and country of publication, patient group studied, study type, relevant outcomes and results of these papers were tabulated. The majority of studies trended towards greater incidence of early lung cancer detection, and subsequent curative treatment, in the LDCT screening populations with appropriately powered randomized control trials (NELSON and NLST) demonstrating survival benefits of &gt;20% in lung cancer-specific mortality. However, this reduction must be evaluated against the potential harms associated with screening, including complications from diagnostic procedures, and costs of overdiagnosis, as evidenced in several studies. We conclude that in high-risk populations, lung cancer screening with LDCT results in earlier detection of low-stage cancers and improved survival when compared to usual clinical care or screening with a chest X-ray.
Lives and Costs Saved by Expanding and Expediting Coronavirus Disease 2019 Vaccination
Authors: Bartsch Sarah M , Wedlock Patrick T , O’Shea Kelly J , Cox Sarah N , Strych Ulrich , Nuzzo Jennifer B , Ferguson Marie C , Bottazzi Maria Elena , Siegmund Sheryl S , Hotez Peter J , Lee Bruce Y 
Published: April 2021

Abstract

Background
With multiple coronavirus disease 2019 (COVID-19) vaccines available, understanding the epidemiologic, clinical, and economic value of increasing coverage levels and expediting vaccination is important.

Methods
We developed a computational model (transmission and age-stratified clinical and economics outcome model) representing the United States population, COVID-19 coronavirus spread (February 2020–December 2022), and vaccination to determine the impact of increasing coverage and expediting time to achieve coverage.

Results
When achieving a given vaccination coverage in 270 days (70% vaccine efficacy), every 1% increase in coverage can avert an average of 876 800 (217 000–2 398 000) cases, varying with the number of people already vaccinated. For example, each 1% increase between 40% and 50% coverage can prevent 1.5 million cases, 56 240 hospitalizations, and 6660 deaths; gain 77 590 quality-adjusted life-years (QALYs); and save $602.8 million in direct medical costs and $1.3 billion in productivity losses. Expediting to 180 days could save an additional 5.8 million cases, 215 790 hospitalizations, 26 370 deaths, 206 520 QALYs, $3.5 billion in direct medical costs, and $4.3 billion in productivity losses.

Conclusions
Our study quantifies the potential value of decreasing vaccine hesitancy and increasing vaccination coverage and how this value may decrease with the time it takes to achieve coverage, emphasizing the need to reach high coverage levels as soon as possible, especially before the fall/winter.
Study on novel PtNP–sorafenib and its interaction with VEGFR2
Authors: Liu Ruirui , Meng Yajie , Zhu Min , Zhai Honglin , Lv Wenjuan , Wen Tao , Jin Nengzhi 
Published: April 2021

Abstract
With the developments of nanodrugs, some drugs have combined with nanoparticles (NPs) to reduce their side-effects and increase their therapeutic activities. Here, a novel nanodrug platinum nanoparticle–sorafenib (PtNP–SOR) was proposed for the first time. By means of molecular dynamics simulation, the stability and biocompatibility of PtNP–SOR were investigated. Then, the interaction mechanism between PtNP–SOR and vascular endothelial growth factor receptor 2 (VEGFR2) was explored and compared with that of the peptide 2a coated PtNPs. The results showed that PtNP–SOR could bind to VEGFR2 more stably, which was driven by the Coulombic and strong dispersion interaction between PtNP–SOR and VEGFR2. According to their contributions obtained from the decomposition of binding free energies, the key residues in VEGFR2 were identified to form the specific space, which increased the affinity with PtNP–SOR. This study provided useful insights to the design of PtNP-drugs as well as important theoretical proofs to the interaction between PtNP–SOR and VEGFR2 at a molecular level, which can be of large help during the development and optimization of novel nanodrugs.
Creating Responsible Subjects: The Role of Mediated Affective Encounters
Authors: Bajde Domen , Rojas-Gaviria Pilar 
Published: April 2021

AbstractWhy do people willingly bestow upon themselves the responsibility to tackle social problems such as poverty? Consumer research has provided valuable insight into how individuals are created as responsible subjects but has yet to account for the crucial role of affective dynamics in subject formation. We draw upon affect theorizing and nascent research on “affective governmentality” in organization and policy studies to theorize the formation of responsible subjects via affective encounters (i.e., consumption encounters through which consumers’ capacities to affect and to be affected change), and to explore how affective encounters are mediated downstream. Through a qualitative investigation of the online microloan market, we explain how market intermediaries contribute to the creation of affective-entrepreneurial subjects who willingly supply interest-free loans to the disadvantaged. The intermediaries accomplish this by nurturing and dramatizing a structure of feeling that subtends affective encounters and by deploying apparatuses of affirmation and relatability to target and intervene into affective encounters. In addition to illuminating the affective dynamics involved in consumer responsibilization and subject formation more broadly, our study facilitates critical reflection on the subject-formative power of consumer experiences and experiential marketing and carries important implications for research on charitable giving and critical thinking on microcredit.
Exhibition Making in Crisis: Professional Identity and Radical Museum Exhibition Design in Britain after the Second World War
Authors: Wintle Claire 
Published: April 2021

Abstract
This article examines the shifting professional identities and forms of exhibition design that were activated by the post-war crisis in UK museums. Drawing on professional publications and museum archives across the UK, it focuses on museum exhibition design for ‘ethnographic collections’ between 1945 and 1965. It documents the sense of collections excess that occurred as objects returned from war-time storage to their bomb-damaged museums; it highlights how – as funding, materials and government attention turned to reconstruction elsewhere – museum staff faced this crisis alone.
The article identifies two key tropes in post-war museum exhibition design that relate to this crisis. Both draw upon a ‘makeshift’ approach and both can be aligned with a ‘Do-It-Yourself’ ethic associated with a shortage of professional labour and more democratic modes of production. Firstly, some exhibition makers responded with creativity and innovation, drawing on new materials and commercial and artistic practices to design exhibitions that were radical for museums at this time. Without external support, museum technicians took a leading role in this experimental practice. Secondly, curatorial apathy towards display temporarily carved out inclusive spaces for artists, academics and local communities to design their own exhibitions in ways that remain radical in museums even today.
The Death of Sodium Pentothal: The Rise and Fall of an Anesthetic Turned Lethal
Authors: Orkaby Asher , Desai Sukumar P 
Published: April 2021

Abstract
For nearly a century, sodium pentothal was the undisputed king of anesthetics. Anesthesiologists were not, however, the sole consumers of pentothal, as psychiatrists used it to treat acute anxiety during psychoanalysis. The associated drug-induced inhibitions were attractive not only to psychotherapists, but also to a new generation of policing and Cold War espionage searching for the elusive truth serum. Cameo appearances of pentothal in media, film, and popular culture propagated the anesthetic’s negative public image. While legal challenges to the admissibility of pentothal-induced confessions and congressional investigations of clandestine truth serum programs may have tainted the popular anesthetic, it was pentothal’s widespread adaptation as part of the lethal injection cocktail that finally killed the king of anesthetics as pharmaceutical companies around the world refused to manufacture what had been transformed into a largely unprofitable drug, associated with capital punishment.
Special Economic Zones in International Economic Law: Towards Unilateral Economic Law
Authors: Chaisse Julien , Dimitropoulos Georgios 
Published: April 2021

ABSTRACT
The international economic regime has entered a new phase of reassertion of sovereignty by States. While States continue to show respect for the values of international (economic) law, the institutionalization of these values has devolved from the international (to the regional) to the domestic level of governance. A new form of ‘unilateral economic law’ is thus gaining importance in the development of international and domestic laws and institutions. However, it remains largely understudied. This article discusses the development and proliferation as well as the importance of special economic zones as a new form of unilateral economic law in the overall system of international economic law. This article identifies four types of economic unilateralism: classical unilateralism, embedded unilateralism, sustainability unilateralism, and national security unilateralism. The new special economic zone unilateralism represents a middle ground between the two extremes of unilateral liberalization and aggressive unilateralism. Accordingly, special economic zone unilateralism introduces a new layer in the overall system of international economic law. First, special economic zones embody a new compromise between the State and the market. The State-controlled promotion of trade and investment taking place through special economic zones represents a complex compromise between the liberalization and protection of economic sovereignty. Second, the spatiality of trade and investment promotion through special economic zones is different from that of international economic law. The liberalization of trade and investment does not take place for the whole country but for an isolated jurisdiction within the broader national jurisdiction, while the focus is on the supply side rather than the traditional input factors of production. Overall, the new special economic zone unilateralism provides insights into the future of international economic law as envisaged by States. Special economic zones have been employed by States both as an alternative and as a complement to trade and investment promotion through the instruments of international economic law.
Early diving behavior in Weddell seal (Leptonychotes weddellii) pups
Authors: Weitzner Emma L , Pearson Linnea E , Tomanek Lars , Liwanag Heather E M 
Published: April 2021

Abstract
During the dependency period in mammals with parental care, offspring must develop the behavioral skills that allow them to forage independently and thus survive into early adulthood. Deep-diving Weddell seals (Leptonychotes weddellii) are a model species for research on diving physiology, yet previous studies lack a thorough investigation into the diving behavior of dependent pups when they first begin to enter the water. To capture fine-scale dive behavior during the dependency period, we deployed time-depth recorders (TDRs) on Weddell seal pups (n = 18) from the age of 1 week through 7 weeks, during the 2017 and 2019 breeding seasons in McMurdo Sound, Antarctica. Dive parameters were correlated with time of day, age, sex, and weaning status, to characterize diving behavior, and we used raw wet/dry data to determine which times of the day pups were most likely to be in the water. Pups made their deepest and longest dives and had the longest post-dive durations in the morning hours. Pups were in the water more during the late night and morning hours than in the afternoon. Whereas dive depth significantly increased with age, dive duration significantly increased with age and after weaning. Post-dive duration significantly decreased with age and after weaning. We discuss how dependent pups may prioritize the development of swimming and navigational abilities as opposed to building and practicing foraging skills.
Spatial and temporal variability in summer diet of gray wolves (Canis lupus) in the Greater Yellowstone Ecosystem
Authors: Lodberg-Holm Hanna K , Teglas Bonnie S , Tyers Daniel B , Jimenez Michael D , Smith Douglas W 
Published: April 2021

AbstractThe role of predation by large carnivores in suppressing prey populations and structuring ecosystems is highly debated, calling for a detailed understanding of carnivore diets. Wolves (Canis lupus) roam across three continents and persist throughout widely different ecosystems. Their diet is flexible and may vary spatially as well as seasonally, which requires analysis of diet on different spatial and temporal scales. Few studies have investigated the summer diet of wolves, which is more variable, consists of smaller prey, and requires different methods than studying their winter diet. To better understand the summer diet of wolves, we combined three independently collected wolf scat data sets from three distinctly different portions of the Greater Yellowstone Ecosystem: Yellowstone National Park (2009), Grand Teton National Park (2003 – 2009), and the Absaroka-Beartooth Wilderness (2009 – 2010). These areas represent different ecological conditions and management regimes, which may impact wolf diet. We estimated relative biomass and compared occurrence of different prey species among packs, years, as well as the three regions. In total, we analyzed 1,906 wolf scats and found that neonate cervids, adult elk, and adult deer were the most important prey species in the summer diet of the wolves. We found dietary variation among packs residing in the same area, as well as across years. The occurrence of neonate cervids displayed the most variation, and low occurrence of this prey type often was associated with a more diverse diet. Wolf packs within the national parks had a higher occurrence of medium-sized prey (~ 50 – 70 kg) and lower occurrence of small-sized prey (≤ 20 kg) compared to wolves in the Absaroka-Beartooth Wilderness. These results demonstrate flexibility in summer diet across packs, years, and between regions within the Greater Yellowstone Ecosystem.
Discovery of a new extragalactic circular radio source with ASKAP: ORC J0102–2450
Authors: Koribalski Bärbel S , Norris Ray P , Andernach Heinz , Rudnick Lawrence , Shabala Stanislav , Filipović Miroslav , Lenc Emil 
Published: April 2021

ABSTRACT
We present the discovery of another odd radio circle (ORC) with the Australian Square Kilometre Array Pathfinder (ASKAP) at 944 MHz. The observed radio ring, ORC J0102–2450, has a diameter of ∼70 arcsec or 300 kpc, if associated with the central elliptical galaxy DES J010224.33–245039.5 (z ∼ 0.27). Considering the overall radio morphology (circular ring and core) and lack of ring emission at non-radio wavelengths, we investigate if ORC J0102–2450 could be the relic lobe of a giant radio galaxy seen end on or the result of a giant blast wave. We also explore possible interaction scenarios, for example, with the companion galaxy, DES J010226.15–245104.9, located in or projected on to the south-eastern part of the ring. We encourage the search for further ORCs in radio surveys to study their properties and origin.