bluuebunny/crossref_metadata_2025_split huggingface dataset:
SiRNA in MSC-derived exosomes silences CTGF gene for locomotor recovery in spinal cord injury rats
Authors: Huang Wei , Qu Mingjia , Li Lu , Liu Tao , Lin Miaoman , Yu Xiaobing 
Published: June 2021

Abstract
Background
How to obtain a small interfering RNA (siRNA) vector has become a moot point in recent years. Exosomes (Exo) show advantages of long survival time in vivo, high transmission efficiency, and easy penetration across the blood-spinal cord barrier, renowned as excellent carriers of bioactive substances.

Methods
We applied mesenchymal stem cell (MSC)-derived exosomes as the delivery of synthesized siRNA, which were extracted from rat bone marrow. We constructed exosomes-siRNA (Exo-siRNA) that could specifically silence CTGF gene in the injury sites by electroporation. During the administration, we injected Exo-siRNA into the tail vein of SCI rats,

Results
In vivo and in vitro experiments showed that Exo-siRNA not only effectively inhibited the expressions of CTGF gene, but quenched inflammation, and thwarted neuronal apoptosis and reactive astrocytes and glial scar formation. Besides, it significantly upregulated several neurotrophic factors and anti-inflammatory factors, acting as a facilitator of locomotor recovery of rats with spinal cord injury (SCI).

Conclusions
In conclusion, this study has combined the thoroughness of gene therapy and the excellent drug-loading characteristics of Exo for the precise treatment of SCI, which will shed new light on the drug-loading field of Exo.
Adipose-derived mesenchymal stromal cell-derived exosomes promote tendon healing by activating both SMAD1/5/9 and SMAD2/3
Authors: Liu Hengchen , Zhang Mingzhao , Shi Manyu , Zhang Tingting , Lu Wenjun , Yang Shulong , Cui Qingbo , Li Zhaozhu 
Published: June 2021

Abstract
Background
The use of adipose-derived mesenchymal stromal cell-derived exosomes (ADSC-Exos) may become a new therapeutic method in biomedicine owing to their important role in regenerative medicine. However, the role of ADSC-Exos in tendon repair has not yet been evaluated. Therefore, we aimed to clarify the healing effects of ADSC-Exos on tendon injury.

Methods
The adipose-derived mesenchymal stromal cells (ADSCs) and tendon stem cells (TSCs) were isolated from the subcutaneous fat and tendon tissues of Sprague-Dawley rats, respectively, and exosomes were isolated from ADSCs. The proliferation and migration of TSCs induced by ADSC-Exos were analyzed by EdU, cell scratch, and transwell assays. We used western blot to analyze the tenogenic differentiation of TSCs and the role of the SMAD signaling pathways. Then, we explored a new treatment method for tendon injury, combining exosome therapy with local targeting using a biohydrogel. Immunofluorescence and immunohistochemistry were used to detect the expression of inflammatory and tenogenic differentiation after tendon injury, respectively. The quality of tendon healing was evaluated by hematoxylin-eosin (H&E) staining and biomechanical testing.

Results
ADSC-Exos could be absorbed by TSCs and promoted the proliferation, migration, and tenogenic differentiation of these cells. This effect may have depended on the activation of the SMAD2/3 and SMAD1/5/9 pathways. Furthermore, ADSC-Exos inhibited the early inflammatory reaction and promoted tendon healing in vivo.

Conclusions
Overall, we demonstrated that ADSC-Exos contributed to tendon regeneration and provided proof of concept of a new approach for treating tendon injuries.
LigTMap: ligand and structure-based target identification and activity prediction for small molecular compounds
Authors: Shaikh Faraz , Tai Hio Kuan , Desai Nirali , Siu Shirley W. I. 
Published: June 2021

AbstractTarget prediction is a crucial step in modern drug discovery. However, existing experimental approaches to target prediction are time-consuming and costly. Here, we introduce LigTMap, an online server with a fully automated workflow that can identify protein targets of chemical compounds among 17 classes of therapeutic proteins extracted from the PDBbind database. It combines ligand similarity search with docking and binding similarity analysis to predict putative targets. In the validation experiment of 1251 compounds, targets were successfully predicted for more than 70% of the compounds within the top-10 list. The performance of LigTMap is comparable to the current best servers SwissTargetPrediction and SEA. When testing with our newly compiled compounds from recent literature, we get improved top 10 success rate (66% ours vs. 60% SwissTargetPrediction and 64% SEA) and similar top 1 success rate (45% ours vs. 51% SwissTargetPrediction and 41% SEA). LigTMap directly provides ligand docking structures in PDB format, so that the results are ready for further structural studies in computer-aided drug design and drug repurposing projects. The LigTMap web server is freely accessible at https://cbbio.online/LigTMap. The source code is released on GitHub (https://github.com/ShirleyWISiu/LigTMap) under the BSD 3-Clause License to encourage re-use and further developments.
Machine type communications: key drivers and enablers towards the 6G era
Authors: Mahmood Nurul Huda , Böcker Stefan , Moerman Ingrid , López Onel A. , Munari Andrea , Mikhaylov Konstantin , Clazzer Federico , Bartz Hannes , Park Ok-Sun , Mercier Eric , Saidi Selma , Osorio Diana Moya , Jäntti Riku , Pragada Ravikumar , Annanperä Elina , Ma Yihua , Wietfeld Christian , Andraud Martin , Liva Gianluigi , Chen Yan , Garro Eduardo , Burkhardt Frank , Liu Chen-Feng , Alves Hirley , Sadi Yalcin , Kelanti Markus , Doré Jean-Baptiste , Kim Eunah , Shin JaeSheung , Park Gi-Yoon , Kim Seok-Ki , Yoon Chanho , Anwar Khoirul , Seppänen Pertti 
Published: June 2021

AbstractThe recently introduced 5G New Radio is the first wireless standard natively designed to support critical and massive machine type communications (MTC). However, it is already becoming evident that some of the more demanding requirements for MTC cannot be fully supported by 5G networks. Alongside, emerging use cases and applications towards 2030 will give rise to new and more stringent requirements on wireless connectivity in general and MTC in particular. Next generation wireless networks, namely 6G, should therefore be an agile and efficient convergent network designed to meet the diverse and challenging requirements anticipated by 2030. This paper explores the main drivers and requirements of MTC towards 6G, and discusses a wide variety of enabling technologies. More specifically, we first explore the emerging key performance indicators for MTC in 6G. Thereafter, we present a vision for an MTC-optimized holistic end-to-end network architecture. Finally, key enablers towards (1) ultra-low power MTC, (2) massively scalable global connectivity, (3) critical and dependable MTC, and (4) security and privacy preserving schemes for MTC are detailed. Our main objective is to present a set of research directions considering different aspects for an MTC-optimized 6G network in the 2030-era.
Shortest link scheduling in wireless networks under the Rayleigh fading model
Authors: Huang Baogui , Yu Jiguo , Ma Chunmei , Li Fengyin , Li Guangshun 
Published: June 2021

AbstractMany shortest link scheduling algorithms adopt non-fading SINR interference model, which assumes that the received signal power will always remain determinate as long as the transmission power of the corresponding sender is fixed. In fact, because environment always influences the propagation of radio signals, the received signal power is by no means a certain value. Rayleigh fading is a statistical model for radio signals propagation. It assumes that the strength of a signal on a receiver is a random variable, varying with the Rayleigh distribution. This paper proposes a shortest link scheduling algorithm under the Rayleigh fading model (SLSRF). The SLSRF partitions the wireless network area into hexagons and colors the hexagons with three different colors such that two neighboring hexagons have different colors. The senders of the links scheduled simultaneously are arranged in hexagons with the same color. The correctness of the SLSRF is proved through theoretical analysis, and the efficiency is illustrated by elaborate simulations. Our simulation results demonstrate that the schedule delay of SLSRF is less than that of some results under the non-fading SINR interference model. Furthermore, we extend the SLSRF to a distributed version, which is suitable for large wireless networks.
Energy-efficient resource management for OFDM modulated bidirectional relaying with quality-of-service constraint
Authors: Ji Xiaodong , Qian Chong , Huang Chenhao , Ye Shu 
Published: June 2021

AbstractThis paper addressed the energy-efficient resource management problem of an amplify-and-forward relay-assisted bidirectional relay system under a quality-of-service (QoS) constraint. The objective is to develop a holistic resource management algorithm for joint implementation of relay selection, power adaptation and bit-rate management for optimal energy efficiency (EE). A three-stage approach is proposed to solve the energy-efficient resource management problem. At stage 1 (stage of power management), a per-subcarrier energy-efficient problem is investigated, leading to a power adaptation algorithm for maximizing the system EE and ensuring the required level of the system QoS. Within the framework of the power adaptation algorithm and by exploiting spatial diversity of multiple-relay channels, distributed relay selection is investigated at stage 2 (stage of relay management). Next, the bit-rate management problem is tackled at stage 3, namely assigning bit rate to different subcarriers to maximize the system EE further. Finally, summarizing the results achieved at the three stages, a novel EE technology combined with relay selection, bit-rate management and power adaptation is developed. Simulation results validated the correctness and the efficiency of the proposed algorithm. It is shown that the proposed algorithm can significantly reduce the total transmit power of the system while ensuring the required system QoS.
A probiotic has differential effects on allergic airway inflammation in A/J and C57BL/6 mice and is correlated with the gut microbiome
Authors: Casaro Mateus B. , Thomas Andrew M. , Mendes Eduardo , Fukumori Claudio , Ribeiro Willian R. , Oliveira Fernando A. , Crisma Amanda R. , Murata Gilson M. , Bizzarro Bruna , Sá-Nunes Anderson , Setubal Joao C. , Mayer Marcia P. A. , Martins Flaviano S. , Vieira Angélica T. , Antiorio Ana T. F. B. , Tavares-de-Lima Wothan , Camara Niels O. S. , Curi Rui , Dias-Neto Emmanuel , Ferreira Caroline M. 
Published: June 2021

AbstractThe phenotypes of allergic airway diseases are influenced by the interplay between host genetics and the gut microbiota, which may be modulated by probiotics. We investigated the probiotic effects on allergic inflammation in A/J and C57BL/6 mice. C57BL/6 mice had increased gut microbiota diversity compared to A/J mice at baseline. Acetate producer probiotics differentially modulated and altered the genus abundance of specific bacteria, such as Akkermansia and Allistipes, in mouse strains. We induced airway inflammation followed by probiotic treatment and found that only A/J mice exhibited decreased inflammation, and the beneficial effects of probiotics in A/J mice were partially due to acetate production. To understand the relevance of microbial composition colonization in the development of allergic diseases, we implanted female C57BL/6 mice with A/J embryos to naturally modulate the microbial composition of A/J mice, which increased gut microbiota diversity and reduced eosinophilic inflammation in A/J. These data demonstrate the central importance of microbiota to allergic phenotype severity.
Interspecific competition in germination of bird-dispersed seeds in a habitat with sparse tree vegetation in South Africa
Authors: Vukeya L. R. , Mokotjomela T. M. , Malebo N. J. , Oke S. 
Published: June 2021

Abstract
Background
By transporting and scarifying the seeds during ingestion, avian frugivores reduce the competition with siblings, and may improve the germination which is critical for dispersal effectiveness and population recruitment. However, generally, there is limited knowledge on how deposited seeds interact/compete in the new microsite. We tested the hypothesis that the bird-dispersed seeds benefit from improved germination after their passage through the bird’s gut; and we investigated the potential impact of seed density on competition at the microsites by determining whether seed density and species diversity influence germination in the Free State Province, South Africa.

Results
Overall, the results partly supported the hypothesis. Germination trials with defecated seeds of five plant species compared with the manually depulped seeds showed that only Searsia lancea had significantly higher seed germination success and improved germination speed after passage through the bird gut while Ziziphus mucronata only benefited rapid germination. There was a significant correlation between seed size and the germination of bird-ingested seeds except in Olea subsp. africana possibly due to possession of extremely hard protective seed cover. Seed competition experiments pointed to Z. mucronata and O. subsp. africana having significant germination performance that was positively correlated to seed density and seed size while Ehretia rigida did not germinate at all. Seed species diversity in the germination trays did not have a significant impact since the seeds of two former plant species consistently displayed significantly higher germination across the competition levels.

Conclusions
We conclude that different plant species respond differently to seed ingestion by birds, and that further long-term tests for germination physiological responses of the seeds’ samples used in this study are required since poor germination observed in other tree/shrub species cannot be attributed to competition solely.
Prevalence, knowledge and perception of self-medication practice among undergraduate healthcare students
Authors: Akande-Sholabi Wuraola , Ajamu Amen. T. , Adisa Rasaq 
Published: June 2021

Abstract
Background
Globally, self-medication is a common practice, and an increasingly perceived necessity to relieve burdens on health services. However, inappropriate self-medication may result to reduced health outcomes, increased antimicrobial resistance and economic waste. Healthcare students are the future health professionals who will be consistently responsible for educating the public on rational use of medication. This study therefore aimed to assess the prevalence, knowledge and perception of self-medication practices among healthcare students.

Methods
A cross-sectional study was carried out among 866 healthcare students in a Nigerian University, comprising medical, nursing and pharmacy students. Information was garnered from respondents using a self-administered questionnaire. Data were summarized with descriptive statistics, while Chi-square and logistic regression tests were used for categorical variables at p < 0.05.

Results
Mean age was 21 ± 2.8 years, and female respondents were 447 (51.6%). Prevalence of self-medication among respondents was 473 (54.6%). A total of 288 (55.3%) demonstrated good knowledge of self-medication practices, comprising 250 (52.2%) among those who have previously self-medicated and 229 (47.8%) among those who had not. Reasons for engaging in self-medication practices were mentioned to include treatment of minor ailments (357; 32.4%), while 248 (22.5%) believed they had the medical knowledge of what to use. Analgesic (353; 30.1%), antimalarial (352; 30.0%), and antibiotics (182; 15.5%) were the commonest classes of medication used for self-medication. Headache (363; 18.4%), malaria (334; 16.9%), and cough (184; 9.3%) were the most frequently treated conditions. More than half (281; 59.4%) of the respondents’ purchased their self-medicated drugs from the community pharmacy. Gender and respondents’ disciplines were found to be the independent predictors for good knowledge of self-medication practice.

Conclusion
Prevalence of self-medication among the studied healthcare students is moderately high, while approximately half demonstrates good knowledge and perception of self-medication practices. Stimulation for self-medication practice largely arise from the perception of treating minor ailments. This underscores a need for advocacy on responsible self-medication practice during the formal training of these future health professionals, in order to avert its imminent/widespread negative consequences.
Spatial changes in inclusion band spacing as an indicator of temporal changes in slow slip and tremor recurrence intervals
Authors: Nishiyama Naoki , Ujiie Kohtaro , Kano Masayuki 
Published: June 2021

AbstractSlow slip and tremor (SST) downdip of the seismogenic zones may trigger megathrust earthquakes by frequently transferring stress to seismogenic zones. Geodetic observations have suggested that the recurrence intervals of slow slip decrease toward the next megathrust earthquake. However, temporal variations in the recurrence intervals of SST during megathrust earthquake cycles remain poorly understood because of the limited duration of geodetic and seismological monitoring of slow earthquakes. The quartz-filled, crack-seal shear veins in the subduction mélange deformed near the downdip limit of the seismogenic zone in warm-slab environments record cyclic changes in the inclusion band spacing in the range from 4 ± 1 to 65 ± 18 μm. The two-phase primary fluid inclusions in quartz between inclusion bands exhibit varying vapor/liquid ratios regardless of inclusion band spacing, suggesting a common occurrence of fast quartz sealing due to a rapid decrease in quartz solubility associated with a large fluid pressure reduction. A kinetic model of quartz precipitation, considering a large fluid pressure change and inclusion band spacing, indicates that the sealing time during a single crack-seal event cyclically decreased and increased in the range from 0.16 ± 0.04 to 2.7 ± 0.8 years, with one cycle lasting at least 27 ± 2 to 93 ± 5 years. The ranges of sealing time and duration of a cycle may be comparable to the recurrence intervals of SST and megathrust earthquakes, respectively. We suggest that the spatial change in inclusion band spacing is a potential geological indicator of temporal changes in SST recurrence intervals, particularly when large fluid pressure reduction occurs by brittle fracturing.
Endothelial cell regulation of systemic haemodynamics and metabolism acts through the HIF transcription factors
Authors: Lambden Simon , Cowburn Andrew S. , Macias David , Garrud Tessa A. C. , Krause Bernardo J. , Giussani Dino A. , Summers Charlotte , Johnson Randall S. 
Published: June 2021

Abstract
Background
The vascular endothelium has important endocrine and paracrine roles, particularly in the regulation of vascular tone and immune function, and it has been implicated in the pathophysiology of a range of cardiovascular and inflammatory conditions. This study uses a series of transgenic murine models to explore for the first time the role of the hypoxia-inducible factors, HIF-1α and HIF-2α in the pulmonary and systemic circulations as potential regulators of systemic vascular function in normoxic or hypoxic conditions and in response to inflammatory stress. We developed a series of transgenic mouse models, the HIF-1α Tie2Cre, deficient in HIF1-α in the systemic and pulmonary vascular endothelium and the L1Cre, a pulmonary endothelium specific knockout of HIF-1α or HIF-2α. In vivo, arterial blood pressure and metabolic activity were monitored continuously in normal atmospheric conditions and following an acute stimulus with hypoxia (10%) or lipopolysaccharide (LPS). Ex vivo, femoral artery reactivity was assessed using wire myography.

Results
Under normoxia, the HIF-1α Tie2Cre mouse had increased systolic and diastolic arterial pressure compared to litter mate controls over the day–night cycle under normal environmental conditions. VO2 and VCO2 were also increased. Femoral arteries displayed impaired endothelial relaxation in response to acetylcholine mediated by a reduction in the nitric oxide dependent portion of the response. HIF-1α L1Cre mice displayed a similar pattern of increased systemic blood pressure, metabolic rate and impaired vascular relaxation without features of pulmonary hypertension, polycythaemia or renal dysfunction under normal conditions. In response to acute hypoxia, deficiency of HIF-1α was associated with faster resolution of hypoxia-induced haemodynamic and metabolic compromise. In addition, systemic haemodynamics were less compromised by LPS treatment.

Conclusions
These data show that deficiency of HIF-1α in the systemic or pulmonary endothelium is associated with increased systemic blood pressure and metabolic rate, a pattern that persists in both normoxic conditions and in response to acute stress with potential implications for our understanding of the pathophysiology of vascular dysfunction in acute and chronic disease.
Rapid and simple detection of Phytophthora cactorum in strawberry using a coupled recombinase polymerase amplification–lateral flow strip assay
Authors: Lu Xinyu , Xu Heng , Song Wen , Yang Zitong , Yu Jia , Tian Yuee , Jiang Min , Shen Danyu , Dou Daolong 
Published: June 2021

AbstractPhytophthora cactorum is a devastating pathogen that infects a wide range of plants and causes Phytophthora rot disease, which has resulted in great economic losses in crop production. Therefore, the rapid and practicable detection of P. cactorum is important for disease monitoring and forecasting. In this study, we developed a lateral flow recombinase polymerase amplification (LF-RPA) assay for the sensitive visual detection of P. cactorum. Specific primers for P. cactorum were designed based on the ras-related protein gene Ypt1; all 10 P. cactorum isolates yielded positive detection results, whereas no cross-reaction occurred in related oomycete or fungal species. The detection limit for the LF-RPA assay was 100 fg of genomic DNA under optimized conditions. Combined with a simplified alkaline lysis method for plant DNA extraction, the LF-RPA assay successfully detected P. cactorum in naturally diseased strawberry samples without specialized equipment within 40 min. Thus, the LF-RPA assay developed in this study is a rapid, simple, and accurate method for the detection of P. cactorum, with the potential for further application in resource-limited laboratories.