bluuebunny/crossref_metadata_2025_split huggingface dataset:
Factors of mothers' hesitation in discussing reproductive health
Authors: Indraswari Ratih , Shaluhiyah Zahroh , Widjanarko Bagoes , Suryoputro Antono 
Published: October 2021

Reproductive health is always becoming an issue for adolescence. Lack of knowledge, permissive attitude, easy access to pornography and low parental supervision reported as a determinant of risky sexual behavior in adolescence. This study aimed to investigate mother’s perspectives in discussing reproductive health issues with their children aged 9-11 years old. The respondents were 8.046 mothers who had children aged 9-11 years old in Semarang, taken with a purposive sampling technique. Most mother (84.4%) were in adulthood, graduated from Senior High School (50.8%), unemployment (44%), and 47.2% was in low-middle income family. As much as 26.8% of mothers agreed that it is not necessary to inform their children about reproductive health. Talking about reproductive health is taboo for 41.9% of mothers and 38.5% felt awkward. Most of them (71.7%) encountered difficulties in starting reproductive health discussions with their children and 76.1% used other terms to name the genitals to feel more polite. A mother who perceived that delivering reproductive health information is unnecessary (OR 1.44), taboo (OR 1.82), awkward (OR 1.93), and giving courtship permission (OR 1.28) to their children significantly becoming risk factors in practicing reproductive health education. Health workers should help the mothers improve their communication skills, especially in discussing reproductive health with children aged 9-11 years old.
Simulated Atmospheric Response to Four Projected Land-Use Land-Cover Change Scenarios for 2050 in the North-Central United States
Authors: Flanagan Paul Xavier , Mahmood Rezaul , Sohl Terry , Svoboda Mark , Wardlow Brian , Hayes Michael , Rappin Eric 
Published: October 2021

AbstractLand-use land-cover change (LULCC) has become an important topic of research for the central United States because of the extensive conversion of the natural prairie into agricultural land, especially in the northern Great Plains. As a result, shifts in the natural climate (minimum/maximum temperature, precipitation, etc.) across the north-central United States have been observed, as noted within the Fourth National Climate Assessment (NCA4) report. Thus, it is necessary to understand how further LULCC will affect the near-surface atmosphere, the lower troposphere, and the planetary boundary layer (PBL) atmosphere over this region. The goal of this work was to investigate the utility of a new future land-use land-cover (LULC) dataset within the Weather Research and Forecasting (WRF) modeling system. The present study utilizes a modeled future land-use dataset developed by the Forecasting Scenarios of Land-Use Change (FORE-SCE) model to investigate the influence of future (2050) land use on a simulated PBL development within the WRF Model. Three primary areas of LULCC were identified within the FORE-SCE future LULC dataset across Nebraska and South Dakota. Variations in LULC between the 2005 LULC control simulation and four FORE-SCE simulations affected near-surface temperature (0.5°–1°C) and specific humidity (0.3–0.5 g kg−1). The differences noted in the temperature and moisture fields affected the development of the simulated PBL, leading to variations in PBL height and convective available potential energy. Overall, utilizing the FORE-SCE dataset within WRF produced notable differences relative to the control simulation over areas of LULCC represented in the FORE-SCE dataset.
Response to antiseizure medications in epileptic patients with malformation of cortical development
Authors: Chen Wei , Jin Bo , Aung Thandar , He Chenmin , Chen Cong , Wang Shan , Ding Yao , Ding Fang , Wang Chao , Li Hong , Jiang Biao , Zheng Zhe , Dai Haibin , Zhu Junming , Geng Yu , Ding Meiping , Wang Shuang 
Published: October 2021

Background: Malformation of cortical development (MCD) is one of the most common causes of pharmacoresistant epilepsy. Improving the knowledge of antiseizure medications (ASMs) treatment response in epileptic patients with MCD is crucial for optimal treatment options, either pharmacological therapy or non-pharmacological intervention. Aim: To investigate the patterns of medical treatment outcome and the predictors for seizure freedom (SF) with ASM regimens in epilepsy caused by MCD. Methods: The epileptic patients with MCD were consecutively enrolled from March 2013 to June 2019. SF was defined as no seizures for at least 12 months or three times the longest pretreatment inter-seizure interval, whichever was longer. Outcomes were classified into three patterns: pattern A: patients achieved SF at one point and remained so throughout follow-up; pattern B: patients’ seizures fluctuated between periods of SF and relapse; pattern C: SF never attained. The terminal SF was defined if the patients remained SF at the last follow-up visit. Results: A total of 164 epileptic patients with MCD were included. Pattern A was observed in 22, pattern B in 42, and pattern C in 100 patients. SF was ever achieved in 64 (pattern A and B) patients. Twenty-nine patients had terminal SF after a median follow-up time of 4.3 years. With continuing ASM treatment, seizure relapse risk was very low after a 5-year seizure-free period. The pretreatment seizure frequency was the only independent predictor for pattern A and seizure relapse. Sodium channel blockers monotherapy (33.8%) was more effective than levetiracetam (4.5%) in rendering SF in the initial ASM regimen. Conclusion: Medical treatment can be successful in a minority of epileptic patients with MCD, and pretreatment seizure frequency helps to predict the treatment outcome. An unequal efficacy of ASMs in epilepsy caused by MCD suggests etiological evaluation is vital in the management of focal epilepsy.
Comparison of colors, microstructure, chemical composition and thermal properties of bamboo fibers and parenchyma cells with heat treatment
Authors: Wu Jieyu , Zhong Tuhua , Zhang Wenfu , Shi Jiangjing , Fei Benhua , Chen Hong 
Published: October 2021

AbstractThe effects of heat treatment at various temperatures on mechanically separated bamboo fibers and parenchyma cells were examined in terms of color, microstructure, chemical composition, crystallinity, and thermal properties. The heat-treated parenchyma cells and fibers were characterized by scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FTIR), chemical composition analysis, and thermogravimetric analysis (TGA). The results revealed that the colors of bamboo fibers and parenchyma cells were darkened as treatment temperature increased. The microstructure of the treated fibers and parenchyma cells slightly changed, yet the shape of starch granules in parenchyma cells markedly altered at a temperature of above 160 °C. The chemical compositions varied depending on the heat treatment temperature. When treated at 220 °C, the cellulose content was almost unchanged in fibers but increased by 15% in parenchyma cells; the hemicellulose content decreased and the lignin content increased regardless of fibers and parenchyma cells. The cellulose crystal structure was nearly unaffected by heat treatment, but the cellulose crystallinity of fibers changed more pronouncedly than that of parenchyma cells. The thermal stability of parenchyma cells after heat treatment was affected more substantially compared to fibers.
From digestion and absorption to innate immunity and health care: water and food intake may contribute to IL-22 in ILC3-dependent mucosal immunity in the jejunum
Authors: Watanabe-Asaka Tomomi , Hayashi Moyuru , Maejima Daisuke , Kawai Yoshiko , Ohhashi Toshio 
Published: October 2021

AbstractIn this review, with our current studies we demonstrated medical evidence that water and food intake are useful for IL-22-related mucosal immunity-dependent maintenance of health care. The traditional Japanese health care practices recommend daily consumption of suitable volume of water. However, immunological mechanisms that support of the traditional practices are still unsolved. We focused on type 3 innate lymphoid cells (ILC3s), because the ILC3s are mainly housed in the lamina propria of the jejunum. IL-22 released from the ILC3 is transported through mesenteric lymph in collaboration with the albumin-mediated movement of consumed water. Thus, water intake-mediated upregulation of IL-22-dependent mucosal immunity contributes to the traditional Japanese health care practices. We also reviewed current studies that food intake-mediated increase in VIP-dependent neuronal activity in the small intestine and the food intake included with tryptophan-derived metabolites may accelerate the IL-22 in ILC3s-dependent mucosal immunity and then contribute in keeping health care.
Circall: fast and accurate methodology for discovery of circular RNAs from paired-end RNA-sequencing data
Authors: Nguyen Dat Thanh , Trac Quang Thinh , Nguyen Thi-Hau , Nguyen Ha-Nam , Ohad Nir , Pawitan Yudi , Vu Trung Nghia 
Published: October 2021

AbstractBackgroundCircular RNA (circRNA) is an emerging class of RNA molecules attracting researchers due to its potential for serving as markers for diagnosis, prognosis, or therapeutic targets of cancer, cardiovascular, and autoimmune diseases. Current methods for detection of circRNA from RNA sequencing (RNA-seq) focus mostly on improving mapping quality of reads supporting the back-splicing junction (BSJ) of a circRNA to eliminate false positives (FPs). We show that mapping information alone often cannot predict if a BSJ-supporting read is derived from a true circRNA or not, thus increasing the rate of FP circRNAs.ResultsWe have developed Circall, a novel circRNA detection method from RNA-seq. Circall controls the FPs using a robust multidimensional local false discovery rate method based on the length and expression of circRNAs. It is computationally highly efficient by using a quasi-mapping algorithm for fast and accurate RNA read alignments. We applied Circall on two simulated datasets and three experimental datasets of human cell-lines. The results show that Circall achieves high sensitivity and precision in the simulated data. In the experimental datasets it performs well against current leading methods. Circall is also substantially faster than the other methods, particularly for large datasets.ConclusionsWith those better performances in the detection of circRNAs and in computational time, Circall facilitates the analyses of circRNAs in large numbers of samples. Circall is implemented in C++ and R, and available for use at https://www.meb.ki.se/sites/biostatwiki/circall and https://github.com/datngu/Circall.
The effects of preoperative alcohol, tobacco, and psychological stress on postoperative complications: a prospective observational study
Authors: Myoga Yoshinori , Manabe Haruhiko , Osaki Yoneatsu 
Published: October 2021

AbstractBackgroundPostoperative complications occur frequently, despite progress in anesthetic pharmacology and surgical techniques. Although habits, such as alcohol and tobacco use, and mental health have been studied individually as modifying factors, few studies have examined the relationship between multiple lifestyle choices and postoperative complications in patients undergoing surgery. Hence, this study aimed to investigate the associations between unhealthy lifestyle choices and postoperative complications.MethodsWe included 730 patients who underwent surgery in our department between March 2015 and April 2016. Participants completed preoperative questionnaires, including the Alcohol Use Disorders Identification Test, Fagerström Test for Nicotine Dependence, and tests for psychological stress (6-item Kessler Psychological Distress Scale; Hospital Anxiety and Depression Scale). Multivariable logistic analysis was used to analyze the association of preoperative drug dependence and psychological stress with postoperative complications.ResultsOf the 721 cases analyzed, 461 (64%) were women. The median age of patients was 62 years (interquartile range: 48–71). At the time of surgical decision-making, 429 out of 710 respondents (60%) had a drinking habit, and 144 out of 693 respondents (21%) had a smoking habit during the preceding year. Seventy-nine patients had developed complications. Multivariable analysis revealed that old age (p = 0.020), psychological stress (p = 0.041), and longer anesthesia time (p < 0.001) were significantly associated with postoperative complications. Drinking or smoking variables were not associated with postoperative complications.ConclusionsPreoperative psychological stress, as evaluated with the 6-item Kessler Psychological Distress Scale, is associated with the risk of postoperative complications.
The BrAID study protocol: integration of machine learning and transcriptomics for brugada syndrome recognition
Authors: Morales M. A. , Piacenti M. , Nesti M. , Solarino G. , Pieragnoli P. , Zucchelli G. , Del Ry S. , Cabiati M. , Vozzi F. 
Published: October 2021

AbstractBackgroundType 1 Brugada syndrome (BrS) is a hereditary arrhythmogenic disease showing peculiar electrocardiographic (ECG) patterns, characterized by ST-segment elevation in the right precordial leads, and risk of Sudden Cardiac Death (SCD). Furthermore, although various ECG patterns are described in the literature, different individual ECG may show high-grade variability, making the diagnosis problematic. The study aims to develop an innovative system for an accurate diagnosis of Type 1 BrS based on ECG pattern recognition by Machine Learning (ML) models and blood markers analysis trough transcriptomic techniques.MethodsThe study is structured in 3 parts: (a) a retrospective study, with the first cohort of 300 anonymized ECG obtained in already diagnosed Type 1 BrS (75 spontaneous, 150 suspected) and 75 from control patients, which will be processed by ML analysis for pattern recognition; (b) a prospective study, with a cohort of 11 patients with spontaneous Type 1 BrS, 11 with drug-induced Type 1 BrS, 11 suspected BrS but negative to Na + channel blockers administration, and 11 controls, enrolled for ECG ML analysis and blood collection for transcriptomics and microvesicles analysis; (c) a validation study, with the third cohort of 100 patients (35 spontaneous and 35 drug-induced BrS, 30 controls) for ML algorithm and biomarkers testing.DiscussionThe BrAID system will help clinicians improve the diagnosis of Type 1 BrS by using multiple information, reducing the time between ECG recording and final diagnosis, integrating clinical, biochemical and ECG information thus favoring a more effective use of available resources.Trial registrationClinical Trial.gov, NCT04641585. Registered 17 November 2020,https://clinicaltrials.gov/ct2/show/NCT04641585
Determinants of time-to-disposition in patients who underwent CT for pulmonary embolism: a retrospective study
Authors: Hassan Ali , Al Dandan Omran , Awary Khaled , Bukhamsin Besma , Bukhamseen Reema , Alzaki Alaa , Al-Sulaibeekh Amal , Alsaif Hind S. 
Published: October 2021

Abstract
Background
Pulmonary embolism (PE) is a common life-threatening medical emergency that needs prompt diagnosis and management. Providing urgent care is a key determinant of quality in the emergency department (ED) and time-based targets have been implemented to reduce length of stay and overcrowding. The study aimed to determine factors that are associated with having a time-to-disposition of less than 4 h in patients with suspected PE who underwent computed tomography pulmonary angiography (CT-PA) to confirm the diagnosis.

Methods
After obtaining approval from the ethics committee, we conducted a retrospective observational study by examining CT-PA scans that was performed to rule out PE in all adult patients presenting at the ED between January 2018 and December 2019. Demographic information and clinical information, as well as arrival and disposition times were collected from electronic health records. Multivariable regression analysis was used to identify the independent factors associated with meeting the 4-h target in the ED.

Results
In total, the study involved 232 patients (76 men and 156 women). The median length of stay in the ED was 5.2 h and the 4-h target was achieved in 37% of patients. Multivariable logistic regression analysis revealed that a positive CT-PA scan for PE was independently associated with meeting the four-hour target in the ED (odds ratio [OR]: 2.2; 95% CI: 1.1–4.8). Furthermore, Hemoptysis was the only clinical symptom that served as an independent factor associated with meeting the 4-h target in the ED (OR: 10.4; 95% CI: 1.2–90.8).

Conclusion
Despite the lower number of staff and higher volume of patients on weekends, patients who presented on weekends had shorter stays and were more likely to meet the 4-h target. Careful clinical assessment, prior to requesting a CT-PA scan, is crucial, since negative CT-PA scans may be associated with failure to meet the 4-h target.
Barriers and facilitators to cardiopulmonary resuscitation within pre-hospital emergency medical services: a qualitative study
Authors: Dehghan-Nayeri Nahid , Nouri-Sari Hassan , Bahramnezhad Fatemeh , Hajibabaee Fatemeh , Senmar Mojtaba 
Published: October 2021

AbstractBackgroundOut-of-hospital cardiopulmonary arrest is a common and fatal problem. Rescuing patients with this problem by pre-hospital emergency medical services is associated with various barriers and facilitators. Identifying these barriers as well as the facilitators in a qualitative and an information-rich way will help to improve the quality of performing the maneuver and to increase the patients’ survival. Therefore, the current study was qualitatively conducted with the aim of identifying the factors affecting the cardiopulmonary resuscitation within the pre-hospital emergency medical services.MethodsThis qualitative study was conducted using a content analysis approach in Iran in 2021. The participants were 16 Iranian emergency medical technicians who were selected through a purposive sampling method. For data collection, in-depth and semi-structured interviews were conducted. For data analysis, the Elo and Kyngäs method was applied.ResultsThe mean participants’ age was 33.06 ± 7.85 years, and their mean work experience was 10.62 ± 6.63 years. The collected information was categorized into one main category called “complex context of the cardiopulmonary resuscitation” and 5 general categories with 17 subcategories. These categories and subcategories include patient condition (patient’s underlying diseases, age, high weight, number of children, and place of living), dominant atmosphere in companions at home (companions’ feeling of agitation, companions doing harm, and companions helping), policy (educational policy, human resource policy, up-to-date equipment and technology, and do-not-resuscitate policy), performance of the out-of-organizational system (disorganization in the patient handover process, and cooperation of the support organizations), and conditions related to the treatment team (conscience, cultural dominance, and shift burden).ConclusionsThe results showed that the conditions related to the patient and his/her companions, as well as the organizational factors such as the policies and the out-of-organizational factors act as the barriers and the facilitators to the cardiopulmonary resuscitation within pre-hospital emergency medical services. Therefore, the barriers can be modified and the facilitators can be enhanced by taking various measures such as educating, human resource policy-making, upgrading the equipment, and considering appropriate management policies.
Intestinal microbiota has important effect on severity of hand foot and mouth disease in children
Authors: Shen Chenguang , Xu Yi , Ji Jingkai , Wei Jinli , Jiang Yujin , Yang Yang , Yang Minghui , Huang Huaxin , Zou Rongrong , Fang Chunxiao , Zeng Fansen , Yang Fengxia , Wang Xinfa , Yuan Jing , Li Jianmin , Wang Xianfeng , Yang Huanming , Gong Sitang , Wang Hui , Xia Huimin , Ma Jinmin , Liu Yingxia 
Published: October 2021

AbstractBackgroundThe incidence of hand foot and mouth disease (HFMD) has increased in recent years, making it a very common childhood illness worldwide. The relationship between different enterovirus genotypes and disease severity is not clearly understood. Given that enteroviruses are transmitted through the gastrointestinal tract, we hypothesized that variation in intestinal microorganisms of the host might play a role in the prognosis of HFMD.MethodsWe carried out a meta-transcriptomic-wide association study of fecal samples obtained from a cohort of children (254 patients, 227 tested positive for enterovirus, including 16 patients co-infectied with 2 kinds of enterovirus) with mild and severe HFMD and healthy controls.ResultsWe found there was no significant difference in the amount of each virus type between the mild and severe cases. Genes of enterovirus 71 (EV71) and coxsackievirus A (CV-A) from the severe and mild cases did not show significant clustering.Clostridiumsp. L2-50 andBacteroides stercorisATCC 43183 were enriched in the guts of children with severe HFMD and KEGG enrichment was found between mild and severe cases.ConclusionsIntestinal microorganisms appear to interact with enterovirus to determine the progression of HFMD. Genes ofBacteroidesandClostridiummay be used as predictive markers for a more efficient prognosis and intervention. The enrichment of intestinal bacteria genes with functions may facilitate the development of severe symptoms for HFMD patients.
A novel gene expression signature-based on B-cell proportion to predict prognosis of patients with lung adenocarcinoma
Authors: Zhang Yi , Yin Xuewen , Wang Qi , Song Xuming , Xia Wenjie , Mao Qixing , Chen Bing , Liang Yingkuan , Zhang Te , Xu Lin , Jiang Feng , Xu Xinyu , Dong Gaochao 
Published: October 2021

Abstract
Background
This study aimed to develop a reliable immune signature based on B-cell proportion to predict the prognosis and benefit of immunotherapy in LUAD.

Methods
The proportion of immune cells in the TCGA-LUAD dataset was estimated using MCP-counter. The Least Absolute Shrinkage and Selector Operation was used to identify a prognostic signature and validated in an independent cohort. We used quantitative reverse transcription-polymerase chain reaction (qRT-PCR) data and formalin-fixed paraffin-embedded (FFPE) specimens immunohistochemistry to illustrate the correlation between prognostic signature and leukocyte migration.

Results
We found that the relative abundance of B lineage positively correlated with overall survival. Then, we identified a 13-gene risk-score prognostic signature based on B lineage abundance in the testing cohort and validated it in a cohort from the GEO dataset. This model remained strongly predictive of prognoses across clinical subgroups. Further analysis revealed that patients with a low-risk score were characterized by B-cell activation and leukocyte migration, which was also confirmed in FFPE specimens by qRT-PCR and immunohistochemistry. Finally, this immune signature was an independent prognostic factor in the composite nomogram of clinical characteristics.

Conclusions
In conclusion, the 13-gene immune signature based on B-cell proportion may serve as a powerful prognostic tool in LUAD.