bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Use And Benefits of 3D Digital Microscope In Hepatic Artery Reconstruction During Living Donor Liver Transplant
Authors: Lin Ching-Min , Lin Shih-Lung , Hung Yu-Ju , Hsieh Chia-En , Chen Yao-Li , Chang Chien-Hsiang 
Published: September 2021

Abstract
Background: To compare the intraoperative process of hepatic artery anastomosis using conventional microscope and novel 3D digital microscope and to share our institute’s experience for better surgical training.Method: A retrospective cohort study with 46 hepatic artery reconstructions in living donor liver transplant patients via operational microscope (Control group) and 3D digital microscope Mitaka Kestrel View II (Study group). We then discuss and share our institution’s experience to improve surgical training. Results: Both operation instruments provide effective and comparable results. There was no statistical significance regarding operational objective results between conventional microscope and exoscope. Both instruments have no hepatic artery size limit, and both have complete vessel patency rate. Conclusions: There was no statistical differences regarding hepatic artery anastomosis between microscope and exoscope cohorts. However, exoscope provided better ergonomics in the operation room and lessen musculoskeletal strain, allowing surgeons to work in a more neutral and comfortable posture and allow first assistant to learn and assist more effectively. Using exoscope with micro-forceps and modified tie technique make artery reconstruction easier.
Families’ Experiences of Suicide Victims in South Korea: A Phenomenological Study
Authors: Lee Eun Joo , Lee Soo Jung 
Published: September 2021

Abstract
Background: When a family commits suicide, family members experience feelings of hurt, denial, shock and anger, resentment, shame, and guilt. If left untreated, they become physically and psychologically vulnerable and the risk of suicidal ideation is high, so clinical intervention in the survivors of suicide is required. This study aimed to explore the experiences of families of suicide victims in South Korea.Methods: This research was designed to a qualitative phenomenological study conducted by using Colaizzi’s methods. Participants were 7 bereaved families living in Changwon City, South Korea. Data were collected through in‐depth and individual interviews with participants from June to December 2018.Results: A total of 25 meaning units, 11 theme clusters, and 5 themes emerged. The 5 themes of south Korean bereaved families’ experience were: shock and confusion, pain of loss, disconnection interpersonal relationships, reality wanting to give up, and life to live. Conclusion: The bereaved families are at high risk of suicidal thoughts or suicide attempts, so active individual professional counseling should be provided for them.
Polyunsaturated Fatty Acids ω-3 and ω-6 Regulate the Proliferation Invasion and Angiogenesis of Human Gastric Cancer Through COX/PGE Signaling Pathway
Authors: Ma JIachi , Zhang Chensong , Liang Wanqing , Li Lei , Du Jun , Pan Chengwu , Chen Bangling , Chen Yuzhong , Wang Yuanpeng 
Published: September 2021

Abstract
Background: To investigate the effect of polyunsaturated fatty acids ω-3 and ω-6 and their metabolites prostaglandin PGE2 and PGE3 on the proliferation, invasion and neovascularization of gastric cancer.Methods: RT-PCR and ELISA were used to detect the gene and protein expression of COX-1 and COX-2 in gastric cancer cell lines, respectively. The effects of ω-3, ω-6, PFG2 and PEG3 on the proliferation, invasion and neovascularization of gastric cancer cells were detected by cell proliferation, invasion and neovascularization assay in vitro. COX-2 siRNA was synthesized by short gene interfering RNA (siRNA) technique and transfected into gastric cancer cells, and the expression of COX-2 protein in gastric cancer cell lines was detected again by Western blot. The effects of COX-2 gene silencing on proliferation, invasion and neovascularization of gastric cancer cells were detected by WST-1 assay, transwell chamber assay and gastric cancer neovascularization assay, respectively.Results: COX-2 was only expressed in MKN74 and MKN45 cell lines, while COX-1 was expressed in four gastric cancer cell lines. In gastric cancer cell lines with positive COX-2 expression, ω-6 and PEG2 could significantly enhance the proliferation, invasion and neovascularization of gastric cancer cells, and after transfection with COX-2 siRNA, the effects of ω-6 and PEG2 on enhancing the proliferation, invasion and neovascularization of gastric cancer cells were significantly attenuated; ω-3 and PEG3 could inhibit the proliferation, invasion and neovascularization of gastric cancer cells. In gastric cancer cell lines with negative COX-2 expression, ω-6 and PEG2 had no significant effect on the proliferation, invasion and neovascularization of gastric cancer; ω-3 and PEG3 could significantly inhibit the proliferation, invasion and neovascularization of gastric cancer.Conclusion: ω-6 PUFAs reinforce the metastatic potential energy of gastric cancer cells via COX-2/PGE2; ω-3 PUFAs inhibit the metastatic potential energy of gastric cancer via COX-1/PGE3.
Surgery versus intervention in congenital heart disease as a risk factor for executive dysfunction: a case control study
Authors: Mani Arash , Nasiri Maryam , Amoozgar Hamid , Amirghofran Ahmad Ali , Nejati Vahid 
Published: September 2021

Abstract
Background

Improving surgical and interventional modalities for treating congenital heart disease increased these children's living expectancy, a leading cause of cognitive and behavioral problems. This study compared executive functions among surgically, interventionally treated congenital heart disease, and aged match control.
Methods

This was a retrospective cross-sectional study. We enrolled thirty children treated with surgery, 30 interventionally treated patients and 30 healthy controls. We compared these participants in the executive functions domains by Stroop test, trail making test, cancellation test, visual and auditory memory span test, and behavioral problems using the parent form strength and difficulty questionnaire. Executive performance in memory span and sustained attention was lower in the surgical group than in the intervention group (P˂0.05).
Results

Both groups of patients had lower flexibility, memory recall, simple and complex working memory, visual attention, and simple and complex selective attention performance (P < 0.05). The effect of behavioral problems on daily life was higher in surgical patients than in the intervention group (P˂0.0001). Both groups of patients had higher emotional problems, conduct problems, and communication problems than the normal group (P˂0.0001).
Conclusion

Interventionaly treated patients had better performance in comparison to surgically-treated patients. Regardless of the severity of the disease or treatment, patients with congenital heart malformations had poor performance compared to normal subjects and suffered from behavioral disorders affecting their daily lives. It is essential to include diagnostic and therapeutic interventions in treating these patients' executive function and behavioral problems.
Design and Selection of Pyrazolo[3,4-d][1,2,3] Triazole-based High-energy Materials
Authors: Jin Xinghui , Liu Luhao , Zhou Jianhua , Hu Bingcheng 
Published: September 2021

Abstract
In this study, we design a series of bridged energetic compounds based on pyrazolo[3,4-d][1, 2, 3]triazole to screen potential energetic materials with excellent detonation properties and acceptable sensitivities. The electronic structures, heats of formation, detonation velocity, detonation pressure, and impact sensitivity of the designed compounds were calculated using density functional theory. The results showed that the designed compounds have high positive heats of formation in the range of 1035.4 (A7) to 2851.4 kJ mol−1 (D2). Moreover, the designed compounds have high crystal densities and heats of detonation, which significantly enhance detonation pressures and velocities. The detonation pressures and velocities are in the ranges of 6.23 (A1) to 9.65 km s−1 (D3) and 15.7 to 43.9 GPa (E8), respectively. The impact sensitivity data also suggest that the designed compounds have impact sensitivities in an acceptable range. Considering detonation pressures, detonation velocities, and impact sensitivities, six compounds (C3, C5, D3, D5, E3, and F3) were screened as potential materials with high energy density, excellent detonation properties, and low impact sensitivities. Finally, the electronic structures of the screened compounds were simulated to provide further understanding on the physicochemical properties of these compounds.
Theoretical Investigation of the Modeling of Indole –dichlormethane Hydrogen Bonding in Different Solvents
Authors: Al-Otaibi Jamelah S. , Mary Y.Sheena , Mary Y.Shyma 
Published: September 2021

Abstract
Theoretical vibrational spectra of indole (IND) and its binary combination with dichloromethane (DC) in various solvents were computed to track the impact of molecular interactions on drug spectral characteristics. When transitioning from plain drug to complexes, 3561.26 cm− 1 mode of the IND displays a substantial shift in peak location. The 3561.26 band shows (~ 15.58) red shift upon dilution. The geometry of drug and IND-DC complex in various solvents was calculated using quantum chemical calculation utilizing DFT.
Encapsulation of Rat Bone Marrow Derived Mesenchymal Stem Cells (rBMMSCs) in Collagen Type I Containing Platelet-Rich Plasma for Osteoarthritis Treatment in Rat Model
Authors: Ebrahimi-barough somayeh , Islam Md Shahidul , Mahtab Mamun Al , Shirian Sadegh , Aghayan Hamid Reza , Arjmand Babak , Allahverdi Amir , Ranjbar Faezeh Esmaeili , Sadegh Amin Bigham , Ai Jafar 
Published: September 2021

Abstract
Osteoarthritis (OA) is the most common form of degenerative joint disease, affecting more than 25% of the adult though prevalent in the elderly population. Most of the current therapeutic modalities aim at symptomatic treatment and lingering the disease progression. In recent years, regenerative medicine such as stem cell transplantation and tissue engineering has been suggested as a potential curative intervention for OA. The objective of current study was to assess the safety and efficacy of an injectable tissue-engineered construct composed of BMMSCs, PRP, and Collagen type I in rat model of OA.To produce collagen type I, PRP and BMMSCs, male Wistar rats were ethically euthanized. After expansion and characterization of rat BMMSCs (rBMMSCs), tissue-engineered construct was formed by combination of appropriate amount of collagen type I, PRP and rBMMSCs. In vitro studies were conducted to evaluate the effect of PRP on chondrogenic differentiation capacity of encapsulated cells. Then tissue-engineered construct was injected in knee joint of rat model of OA (24 rat in 4 groups:OA, OA+MSC, ‎OA+Collagen+MSC+PRP, OA+MSC+Collagen).After 6 weeks, the animals were euthanized and knee joint histopathology examinations were performed to evaluate the effect of each treatment on OA.Tissue-engineered construct was successfully manufactured and in vitro assays demonstrated that relevant chondrogenic genes and proteins expression were higher in PRP group than the others. Histopathological findings of the knee joint samples showed favorable regenerative effect of rBMMSCs+PRP+Collagengroup comparing to others.In this study, we introduced an injectable tissue-engineered product composed of rBMMSCs+PRP+Collagenwith potential regenerative effect on cartilage damage caused by OA.
LncRNA-ZFAS1 Induces Epithelial-Mesenchymal Transition of Pancreatic Cancer Cells During Nutrient Deprivation by Promoting AMPK-Mediated Phosphorylation and Stabilization Of ZEB1 Protein
Authors: ZENG ZHU , Han Shengbo , Wang Yang , Huang Yan , Hu Yuhang , Xu Fengyu , Zhao Yong , Huang Mengqi , Tang Jiang , Wang Decai , Zhao Gang 
Published: September 2021

Abstract
Background: Nutrient deprivation is a distinct feature of the tumor microenvironment that plays a crucial role in various cancers. However, the contribution and regulatory mechanism of nutrient deprivation on metastasis of pancreatic cancer (PC) have not been identified. Methods: PC cells were treated with normal medium, glucose-depletion or glutamine-depletion medium to observe the epithelial-mesenchymal transition (EMT). RT-qPCR and western blot assay were applied to evaluate the alteration of mRNA and protein of zinc finger E-box binding homeobox 1 (ZEB1), a crucial EMT regulator factor. Co-IP assay was utilized for evaluating the interaction between AMP-activated protein kinase (AMPK) and ZEB1. LncRNA microarray was adopted to detect the potential lncRNA, which facilitates the association between AMPK and ZEB1. Gain- and loss-of-function experiments were performed to evaluate the roles of ZNFX1 antisense RNA 1 (ZFAS1) in EMT and metastasis of PC. Results: The present study reveals that nutrient deprivation including glucose and glutamine deprivation significantly induces EMT of PC cells, which is dependent on stabilization of ZEB1. We further discover that nutrient deprivation induces upregulation of lncRNA ZFAS1, which promotes the association between AMPK and ZEB1 to phosphorylate and stabilize ZEB1 protein. Notably, ZEB1 reciprocally promotes the transcription of ZFAS1 by binding to the promoter of ZFAS1, forming feedback with ZFAS1. Consistently, depletion of ZFAS1 obviously inhibits nutrient deprivation-induced EMT of PC cells and lung metastasis of PC in nude mice. Meanwhile, clinical data displays that ZFAS1 is overexpressed in PC tissues and correlated with high expression of ZEB1 and Vimentin (VIM), low expression of E-cadherin (E-cad), as well as poor prognosis in PC patients. Conclusions: Our study implicates that glucose and glutamine deprivation promotes EMT and metastasis of PC through lncRNA-mediated stabilization of ZEB1.
Effects of combined intervention of computer-aided self-regulation learning (CA-SRL) and cognitive training on generalization ability and cognitive function in post-stroke patients with cognitive impairment: a randomized controlled trial
Authors: He Youze , Yang Ting , Bao Yaqi , Xiao Tianshen , Wu Tiecheng , Wu Jingsong 
Published: September 2021

Abstract
Emerging study suggests the application of self-regulation learning (SRL) for improving generality abilities in post-stroke patients. It is proposed that SRL could generate an added effect on computer-aided cognitive training (CACT). This study aimed to examine the efficacy of the combined intervention of computer-aided SRL (CA-SRL) training and CACT for generalization ability and cognitive function in patients with post-stroke cognitive impairment (PSCI). A total of 75 patients were randomly assigned into CA-SRL group, demonstration learning (DL) group and traditional learning (TL). Over three weeks, patients in these three groups underwent CA-SRL or DL training combining with cognitive training respectively. After intervention, all outcomes have significantly improved after intervention (P < 0.05). CA-SRL group showed better improvements in all trained tasks among groups, especially in "wash the dishes" and “change the bed”. The analysis of generalization ability found CA-SRL groups obtained the highest scores among groups in untrained tasks. Mean changes of MoCA in both CA-SRL and TL groups were significantly higher than the DL group (P < 0.001, P = 0.002) after adjusting education year and Lawton Instrumental ADL Scale. In general, the combination of CA-SRL and CACT is effective for PSCI patient and it has a better effect in promoting the skills generalization from cognitive gains than traditional training.
Cinnabarinic Acid from Trametes Coccinea Fruiting Bodies Exhibits Antibacterial Activity Through Inhibiting the Biofilm Formation
Authors: Kakoti Merilin , Dullah Samim , Hazarika Dibya Jyoti , Barooah Madhumita , Boro Robin Chandra 
Published: September 2021

Abstract
Wild mushrooms are rich sources of natural compounds with potent bioactive properties. Several important metabolites have been reported from mushrooms, which possess clinically important bioactive properties like antibacterial activity, anticancer activity, antidiabetic activity and antioxidant activity. In this study, we have evaluated the antimicrobial activity of Trametes coccinea fruiting body extracts against different bacterial isolates viz. Bacillus subtilis, Bacillus cereus and Escherichia coli. Fruiting bodies of three T. coccinea samples, of which two were collected from Santipur, Arunachal Pradesh and one collected from Jorhat, Assam, were used for extraction using methanol. The extracts showed potent antimicrobial activity against all the test bacteria. Minimum Inhibitory Concentration (MIC) of the extracts against Bacillus subtilis, Bacillus cereus and Escherichia coli was recorded as 400 µg/ml, 400 µg/ml and 300 µg/ml respectively. Further, the bioactive compounds of the extract were separated and detected using Thin Layer Chromatography (TLC). Presence of Cinnabarinic acid (CBA), a potent antimicrobial compound was detected in TLC, which was further confirmed through High Performance Liquid Chromatography (HPLC) and Electrospray Ionization-Mass Spectrometry (ESI-MS). Cinnabarinic acid was able to inhibit the formation of biofilms in Bacillus subtilis and B. cereus, suggesting that the compound can be beneficial in the management of biofilm based antimicrobial resistance.
The Effect of Oxidation Temperature on the (1000–1300) Cm−1 Band in FT-IR Spectra of Silicon Oxide Synthesized by Thermal Oxidation of Silicon Wafers
Authors: Kayed Kamal , Kurd Dalal Baba 
Published: September 2021

Abstract
In this work, silicon wafers were thermal treated in air at temperatures varied in the range 800–1200°C. The annealed samples were investigated using FTIR, X-ray diffraction, FTIR and optical reflection spectroscopy. The effect of annealing temperature on the (1000–1300) cm− 1 band in FT-IR spectra of the prepared samples was investigated. The results showed that thermal oxidation at temperatures less than 1200°C leads to enhance the phenomenon of the splitting of longitudinal optical and transverse optical stretching motions. This phenomenon was verified by observing the appearance of two overlapping peaks in the region (1000–1300) cm− 1 in FT-IR spectra. The results also showed that the splitting process leads to the formation of defects in the crystal structure of silicon, which in turn leads to the formation of silicon nanoparticles.
Evaluation of e-health literacy with the DMVEC.it website in undergraduate student: a pre-test and post-test study
Authors: Conte Alessandro , Brunelli Laura , Moretti Valentina , Valdi Giulia , Guelfi Maria Renza , Masoni Marco , Anelli Filippo , Parpinel Maria , Arnoldo Luca 
Published: September 2021

Abstract
Background

The struggle against fake medical news, especially spread by web sources, is a main issue in public health. Even among college students, there is a lack of eHealth literacy (eHL) skills. The Italian Medical Doctors Federation (FNOMCeO) promoted a website as a first-aid communication kit for notions in health hot-topics named “dottoremaeveroche” (DMVEC). This study aims to evaluate its effectiveness in improving eHL.
Methods

Between April 2019 and October 2020, college students attending the first year of medical and communication theory course at the University of Florence (Italy) joined a web-based survey before and after accessing the DMVEC website. The 8-item self-assessment IT-eHEALS tool was used to examine subject’s eHL, in addition to questions on source’s features and its quality. All responses were rated on a 5-points Likert scale. Changing of abilities perception was assessed using Wilcoxon test.
Results

362 students joined the survey, 329 medical and 33 communication ones. Participants felt moderately confident in eHL, with an initial IT-eHEALS overall mean score of 3.6 ± 0.7 for medical students and of 3.2 ± 0.8 for communication students. Medical students had a good perception on how to find helpful health sources (mean score of 3.9 ± 0.8) and communication students felt sure in discerning sources quality (mean score of 3.5 ± 1.0). Instead, their confidence in using web information to make health decisions was low (medical students: mean score of 2.9 ± 1.1; communication students: mean score of 2.8 ± 1.1). All items improved after the use of DMVEC, with overall mean score of IT-eHEALS increasing to 4.3 ± 0.6 (p < 0.01) for medical students and to 4.1 ± 0.8 (p < 0.01) for communication students. Also the items with the lowest scores improved, even if remained the most critical (medical students: mean score of 3.9 ± 1.2; communication students: mean score of 3.6 ± 1.4; p < 0.01).
Conclusions

Low levels of eHL can damage public health efforts, as seen during COVID19 pandemic. DMVEC effectiveness in college students demonstrated the benefit of online educational interventions that, with further implementation, could help in tackling infodemic and fake news spreading.
Molecular epidemiology of community-onset Clostridioides difficile infections at a tertiary hospital in mainland China: A ten-year (2010-2019) retrospective study
Authors: Chen Yunbo , Bu Lihong , Lv Tao , Zheng Lisi , Gu Silan , He Jianqin 
Published: September 2021

Abstract
Background

Clostridioides difficile infection (CDI) is an increasingly common disease in healthcare facilities and community settings. However, there are limited reports of community-onset CDI (CO-CDI) in China. We retrospectively analyzed the molecular epidemiology of CO-CDI at a tertiary hospital over a period of 10 years.
Methods

A total of 1307 stool samples from 1213 outpatients were tested by culturing. The presence of toxin genes (tcd A, tcd B, cdtA and cdtB) were confirmed by PCR. Toxigenic strains were typed using multilocus sequence typing (MLST). Susceptibility to 9 antimicrobials was evaluated using the E-test.
Results

Eighty-nine of 1213 outpatients (7.3%) had CO-CDI, 4 of these patients (4.5%) had one or more recurrence, and there were 95 strains of toxigenic C. difficile. Among these strains, 82 (86.3%) had the tcdA and tcdB genes (A + B+) and 5 of these 82 strains were positive for the binary toxin genes (cdtA and cdtB); the other 13 strains (13.7%) had the tcdB gene only (A-B+). There were 15 different STs and the most prevalent were ST-54 (23.2%), ST-35 (16.8%), and ST-2 (13.7%). All strains were susceptible to metronidazole and vancomycin, and had low resistance to moxifloxacin and tetracycline, but had high resistance to ciprofloxacin, clindamycin, and erythromycin. Twenty-three isolates (24.2%) were multidrug-resistant.
Conclusions

Outpatients with CDI were common during this period in our hospital. The C. difficile isolates had high genetic diversity. All isolates were susceptible to metronidazole and vancomycin, and nearly one quarter of all isolates had multidrug resistance.