bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification of Candidate Genomic Regions for Chicken Egg Production Traits Based on Genome Wide Association Study
Authors: Zhao Xiurong , Nie Changsheng , Zhang Jinxin , Li Xinghua , Zhu Tao , Guan Zi , Chen Yu , Wang Liang , Lv XueZe , Yang Weifang , Jia Yaxiong , Ning Zhonghua , Li Haiying , Qu Changqing , Wang Huie , Qu Lujiang 
Published: January 2021

Abstract
Background: Since the domestication of chicken, various chicken breeds have been developed for food production, entertainment, and so on. Compared to indigenous chicken breeds which generally do not show elite production performance, commercial breeds or lines are selected intensely for meat or egg production. In the present study, in order to understand the molecular mechanisms underlying the dramatic egg yielding differences between commercial egg-type chickens and indigenous chickens, we performed a genome-wide association study (GWAS) in a mixed linear model. Results: We obtained 148 single nucleotide polymorphisms (SNPs) associated with egg production traits or reproductive traits (57 significantly, 91 suggestively). Among them, 18 SNPs overlapped with previously reported quantitative trait loci (QTL), including 13 for egg production and 5 for reproductive traits. Three SNPs were significantly associated with multiple egg production traits, such as egg number, age at first egg, and egg production rate in chickens. Furthermore, we identified 32 candidate genes based on the function of the screened genes. These genes were found to be mainly involved in regulating hormones, playing a role in the formation, growth, and development of follicles, and in the development of the reproductive system. Some genes such as NELL2, KITLG, GHRHR, NCOA1, ITPR1, GAMT, and CAMK4 deserve our attention and further study since they have been reported to be closely related to reproductive traits. In addition, the most significant genomic region obtained in this study was located at 48.61-48.84Mb on GGA5. In this region, we have repeatedly annotated four genes, in which YY1 and WDR25 have been shown to be related to oocytes and reproductive tissues, respectively, which implies that this region may be a candidate region underlying egg production traits. Conclusion: Our study utilized the genomic information from various chicken breeds or populations differed in egg production to understand the molecular genetic mechanisms involved in reproduction traits. We identified a series of SNPs, candidate genes, or genomic regions that associated with reproductive traits, which could help us in developing egg production in chickens.
The Inhibition of Proliferation and Invasion of Human Colon Carcinoma Cell Line (Caco-2 cells) by Cell-free Supernatants from Lactobacillus Rhamnosus and Lactobacillus Acidophilus
Authors: Shokati Ameneh , Soleimani Masoud , Abroun Saeid 
Published: January 2021

Abstract
Background: The epidemiological studies indicated that colorectal cancer is one of the most common types of cancer in the world and is considered a leading cause of cancer-related death. The present study aimed to investigate the inhibitory effect of lactobacillus acidophilus supernatant (LAS) and lactobacillus rhamnosus supernatant (LRS) on the growth and invasiveness of the human colon carcinoma cell line (Caco2) in-vitro. Methods: In this experimental study, the anti-proliferative activity and anti-invasion potential of LAS and LRS were determined by MTT and transwell chambers assays, respectively. The expression of mitochondrial membrane potential-9 (MMP-9) and matrix metalloproteinase-12 (MMP12) genes were analyzed by real-time PCR.Results: The results indicated that supernatants of these two lactobacilli had cytotoxic effects on Caco-2 cells at a concentration of 25% v/v and higher. Thus, the minimum concentrations (25% V/V) of supernatants were chosen for further experiments. LAS and LRS could significantly suppress the invasiveness of Caco-2 cells. Also, the expression of MMP12 was significantly increased in Caco-2 cells when treated with LAS, whereas LRS had no significant effect on the invasive capacity and the gene expression levels of MMP12. The expression of MMP-9 was statistically decreased in Caco2 cells treated with LAS and LRS (P<0.00001).Conclusion: In general, it was shown that LAS and LRS exert anti-cancer activity against the growth, invasion, and metastasis of Caco2 cells in-vitro. It seems that these two bacteria could be used as prophylactic and therapeutic agents for the prevention and treatment of colorectal cancer.
Determinants of the Continuum of Maternal Health Care in Cambodia: an analysis of the Cambodia Demographic Health Survey 2014
Authors: Chham Savina , Radovich Emma , Buffel Veerle , Ir Por , Wouters Edwin 
Published: January 2021

Abstract
Introduction: Cambodia has achieved significant progress in maternal health, yet remains in the group of countries with the highest maternal mortality ratio in South-East Asia. Extra efforts are needed to improve maternal health through assessing the coverage of maternal health services as a continuum of care (CoC) and identifying the gaps. Our study aims to explore the coverage level of the Optimal CoC by (1) measuring the continuity of optimal antenatal care (ANC), skilled birth attendance (SBA) and optimal post-natal care (PNC), (2) identifying the determinants of dropping out from one service to another and (3) of not achieving the complete CoC. Method: The study employed data from the Cambodia Demographic Health Survey 2014. We restricted our analysis to married women who had a live birth in the five years preceding the survey (n=5678). Bi-variate and multivariate logistic regression were performed using STATA version 14. Results: Almost 50% of women had achieved the complete optimal CoC, while the remaining have used only one or two of the services. The result shows that the level of women’s education was positively associated with the use of optimal ANC, the continuation to using optimal PNC and achieving the complete CoC. More power of women in household decision making was also positively associated with receiving the complete CoC. The birth order was negatively associated with achieving the complete CoC, while exposure to the mass media and having health insurance increased the odds of achieving the complete CoC. Household wealth consequently emerged as an influential predictor of dropping out and not achieving the complete CoC. Receiving all different elements of ANC care improved the continuity of care from optimal ANC to SBA and from SBA to optimal ANC. Conclusion: The findings urge policy makers to approach maternal health care as a continuum of care with different determinants at each step. Household wealth was found to be the most influential factor, yet the study discovered also other barriers to optimal maternal health care which need to be addressed: future intervention should thus not only aim to increase wealth or health insurance coverage but also stimulate the education of women and empower women to claim power in household decision-making.
The Current Status of Health Promotion Lifestyle and Its Related Factors in Chinese Residents
Authors: Liu Qianqian , Huang Shusheng , Qu Xiaoyuan , Yin Aitian 
Published: January 2021

Abstract
Objectives: This study aimed to explore the current status of Chinese residents' health promotion lifestyle and its influencing factors, especially explore how health attitudes affect health promotion lifestyle, thus can make targeted recommendations for health promotion in China and similar areas.Study design: This study was based on a household survey of 1769 adults aged 18 and over from Shandong Province of China conducted in 2018.Methods: A cross-sectional, face-to-face survey design was used. The between-group measured data were compared by One-way ANOVA or t-tests. The correlation between the health attitude and health promotion lifestyle was examined by Pearson correlation. Logistic regression model was used to examine the related factors influencing Chinese residents’ health promotion lifestyle.Results: The health promotion lifestyle of residents in Chinese residents was not good. Significant differences existed in health promotion lifestyle among different genders, education levels, income levels, marital status, and health attitude (Ps <0.001). Multivariable Logistic regression model found that gender, education level, annual family per capita income, affection and behavioral intention in health attitude were key factors influencing Chinese residents’ health promotion lifestyle (Ps<0.001). Conclusions: Individual health responsibilities need to be further strengthened. More affective factors and operable measures should be added to enhance health affection and health behavioral intention, so as to enhance health promotion lifestyle.
Interdependence between histone marks and steps in Pol II transcription
Authors: Wang Zhong , Chivu Alexandra , Choate Lauren , Rice Edward , Miller Donald , Chu Tinyi , Chou Shao-Pei , Kingsley Nicole , Petersen Jessica , Finno Carrie , Bellone Rebecca , Antczak Douglas , Lis John , Danko Charles 
Published: January 2021

Abstract
The role of histone modifications in transcription remains incompletely understood. Here we used experimental perturbations combined with sensitive machine learning tools that infer the distribution of histone marks using maps of nascent transcription. Transcription predicted the variation in active histone marks and complex chromatin states, like bivalent promoters, down to single-nucleosome resolution and at an accuracy that rivaled the correspondence between independent ChIP-seq experiments. Blocking transcription rapidly removed two punctate marks, H3K4me3 and H3K27ac, from chromatin indicating that transcription is required for active histone modifications. Transcription was also required for maintenance of H3K27me3 consistent with a role for RNA in recruiting PRC2. A subset of DNase-I hypersensitive sites were refractory to prediction, precluding models where transcription initiates pervasively at any open chromatin. Our results, in combination with past literature, support a model in which active histone modifications serve a supportive, rather than a regulatory, role in transcription.
Covid-19 Infection in Pregnant Women in Dubai: A Case-control Study
Authors: Hazari Komal , Abdeldayem Rasha , Paulose Litty , Kurien Nimmi , Almahloul Zukaa , Mohammad Hozaifah , Elgergawi Taghrid , Alkhanbouli Maryam , Mahmoud Khalid , Fazari Atif , Hassan Amar , Bayoumi Riad 
Published: January 2021

Abstract
Background: Whilst the impact of Covid-19 infection in pregnant women has been examined, there is a scarcity of data on pregnant women in the Middle East. Thus, the aim of this study was to examine the impact of Covid-19 infection on pregnant women in the United Arab Emirates population. Methods: A case-control study was carried out to compare the clinical course and outcome of pregnancy in 79 pregnant women with Covid-19 and 85 non-pregnant women with Covid-19 admitted to Latifa Hospital in Dubai between March and June 2020. Results: On admission, most pregnant women (69/79; 87%) were either asymptomatic or suffered mild respiratory symptoms, much like non-pregnant women. The main symptoms, observed in both groups, were fever, cough, headache, runny nose and myalgia. In early pregnancy four women had spontaneous miscarriages. However, severe Covid-19 illness, ICU admission, intubation and complications were observed in late pregnancy in 10/79 (13%) women with prior comorbidities. During the study period, 31/79 women delivered: 22 (70%) had a lower segment Caesarean section, 16 for obstetric reasons and 6 for severe Covid-19 pneumonia; and 12 had a preterm delivery. Postoperatively, none showed significant improvement on account of early delivery. Sepsis, acute renal failure and acute respiratory distress syndrome were the most common complications observed. The neonatal outcome was comparable to that of the general obstetric population, even though 12/31 (38%) were preterm and had to be admitted to the neonatal ICU. Two neonates, born by vaginal delivery, tested positive for Covid-19 after delivery. Both were asymptomatic and tests were negative within 72 hours. It was not possible to ascertain whether vertical transmission occurred in utero.Conclusions: Severe Covid-19 illness, ICU admission, intubation, complications and preterm deliveries were observed in late pregnancy in women with comorbidities. Pregnancy with Covid-19 infection, could, therefore, be categorised as high-risk pregnancy. It was not possible to confirm whether vertical transmission of Covid-19 had occurred during the third trimester of pregnancy.
How Imputation Can Mitigate Ascertainment Bias
Authors: Geibel Johannes , Reimer Christian , Pook Torsten , Weigend Steffen , Weigend Annett , Simianer Henner 
Published: January 2021

Abstract
Background Population genetic studies based on genotyped single nucleotide polymorphisms (SNPs) are influenced by a non-random selection of the SNPs included in the used genotyping arrays. The resulting bias relative to whole genome sequencing (WGS) data is known as SNP ascertainment bias. Correction for this bias requires detailed knowledge of the array design process which is often not available in practice. This study intends to investigate an alternative approach to mitigate ascertainment bias of a large set of genotyped individuals by using information of a small set of sequenced individuals via imputation without the need for prior knowledge on the array design. Results The strategy was first tested by simulating additional ascertainment bias with a set of 1,566 chickens from 74 populations that were genotyped for the positions of the Affymetrix Axiom™ 580k Genome-Wide Chicken Array. Imputation accuracy was shown to be consistently higher for populations used for SNP discovery during the simulated array design process. Reference sets of at least one individual per population in the study set led to a strong correction of ascertainment bias for estimates of expected and observed heterozygosity, Wrights Fixation Index and Nei’s Standard Genetic Distance. In contrast, unbalanced reference sets introduced a new bias towards the reference populations. Finally, the array genotypes were imputed to WGS by utilization of reference sets of 74 individuals (one per population) to 98 individuals (additional commercial chickens) and compared with a mixture of individually and pooled sequenced populations. The imputation reduced the slope between heterozygosity estimates of array data and WGS data from 1.94 to 1.26 when using the smaller balanced reference panel and to 1.44 when using the larger but unbalanced reference panel. This generally supported the results from simulation but was less favorable, advocating for a larger reference panel when imputing to WGS. Conclusions The results highlight the potential of using imputation for mitigation of SNP ascertainment bias but also underline the need for unbiased reference sets.
WITHDRAWN: Determination of Minimum Infusion Rate and Cardiorespiratory Effects of Total Intravenous Anesthesia of Ketofol With or Without Lidocaine, Fentanyl or Dexmedetomidine in Dogs
Authors: Rastabi Hadi Imani , Naddaf Hadi , Mosallanejad Bahman , Khannejad Mahmood , Keramat Majid 
Published: January 2021

Abstract
Background: Ketofol is a 1:1 mixture of ketamine and propofol that has been proposed for induction and maintenance of anesthesia aiming to provide more cardiovascular stability and less undesirable impacts compared to the use of propofol and ketamine alone. However, it has been associated with exacerbated respiratory depression in dogs. Diminishing the dose of ketofol may improve cardiovascular effects and attenuate respiratory depression. The present study was designed to evaluate the effect of adding lidocaine, fentanyl or dexmedetomidine at the required dose and cardiorespiratory variables in dogs undergoing total intravenous anesthesia (TIVA) with ketofol. In phase I, twelve dogs were induced and maintained with two out of four anesthetic regimens of KET: ketofol (4 mg/kg and 0.3 mg/kg/min, respectively), KLD; ketofol and lidocaine (1.5 mg/kg and 0.25 mg/kg/min, respectively), KFN: ketofol and fentanyl (LD: 5 µg/kg and 0.1 µg/kg/min, respectively) and KDX: ketofol and dexmedetomidine (2 µg/kg and 2 mg/kg/h, respectively). Minimum infusion rate (MIR) of ketofol was determined in this phase. Subsequently, in phase II, other twelve dogs were given the same anesthetic regimens for 60 min similar to the previous phase, except the infusion rate of ketofol. Cardiorespiratory variables were recorded in predetermined interval. Results: In phase I, mean MIR of ketofol for KET, KLD, KFN and KDX were determined to have decreasing manner as 0.35, 0.23, 0.15, and 0.08 mg/kg/min, respectively. In phase II, the times of recovery events were shorter in KFN and KDX than KET and KLD. Notably, HR was significantly higher than baseline during anesthesia in KET and KLD, which also was significantly lower than baseline in KFN and KDX at several time points. Significant higher values of MAP were observed over time in KDX. In all treatments, there was a decrease in respiratory rate and pH as well as an increase in PCO2 during the anesthesia session. Conclusions: It was concluded that despite decreasing the dose of ketofol, none of the added drugs attenuated respiratory depression caused by ketofol.
Costs of Breast Cancer Treatment Incurred by Women in Vietnam
Authors: Ngan Tran , Ngoc Nguyen , Minh Hoang , Donnelly Michael , O'Neill Ciaran 
Published: January 2021

Abstract
Background: There is a paucity of research on the cost of breast cancer (BC) treatment from the patient’s perspective in Vietnam.Methods: Individual-level data about out-of-pocket (OOP) expenditures on use of services were collected from women treated for BC (n=202) using an online survey and a face-to-face interview at two tertiary hospitals in 2019. Total expenditures on diagnosis and initial BC treatment were presented in terms of the mean, standard deviation, and range for each type of service use. A generalised linear model (GLM) was used to assess the relationship between total cost and socio-demographic characteristics.Results: 19.3% of respondents had stage 0/I BC, 68.8% had stage II, 9.4% had stage III, none had stage IV. The most expensive OOP elements were targeted therapy with mean cost equal to 649.5 million VND ($28,025) and chemotherapy at 36.5 million VND ($1,575). Mean total OOP cost related to diagnosis and initial BC treatment (excluding targeted therapy cost) was 61.8 million VND ($2,667). The mean OOP costs among patients with stage II and III BC were, respectively, 66% and 148% higher than stage I.Conclusions: BC patients in Vietnam incur significant OOP costs. The cost of BC treatment was driven by use of therapies and presentation stage at diagnosis. It is likely that OOP costs of BC patients would be reduced by earlier detection through raised awareness and screening programs and by providing a higher insurance coverage rate for targeted therapy.
An Aqueous, Electrode-Decoupled Redox-Flow Battery for Long Duration Energy Storage
Authors: Sankarasubramanian Shrihari , Zhang Yunzhu , He Cheng , Gregory Thomas , Ramani Vijay 
Published: January 2021

Abstract
Redox-flow batteries (RFBs) enable large-scale energy storage at low cost due to the independent scaling of device power and energy, thereby unlocking energy arbitrage opportunities and providing a pathway to grid stability and resiliency. Herein we demonstrate an “electrode-decoupled” redox-flow battery (ED-RFB) with titanium and cerium elemental actives that has a clear pathway to achieve a levelized cost of storage (LCOS) of ca $0.025/kWh-cycle. A key enabling technology is our highly perm-selective modified poly(ether ketone)-based anion exchange membrane (AEM) that ensures long term separation of Ti and Ce species and enables capacity-fade-free cycling over 1300 hours of operation. Further, our Ti-Ce ED-RFB exhibits negligible capacity fade when the actives are charged to 90% state of charge (SOC), stored for close to 100-hours and then discharged, rendering it viable for long duration (load-following) grid-scale energy storage applications. Herein we introduce the Ti-Ce ED-RFB as a novel, low-cost long duration energy storage (LDES) system.
Delay in Primordial Germ Cell Migration in Adamts9 Knockout Zebrafish
Authors: Carver Jonathan , He Yuanfa , Zhu Yong 
Published: January 2021

Abstract
Adamts9 (a disintegrin and metalloproteinase with a thrombospondin type 1 motif, member 9) is one of few metalloproteinases structurally conserved from C. elegans to humans and is indispensable in germ cell migration in invertebrates. However, adamts9’s roles in germ cell migration in vertebrates has not been examined. In the present study, we found zygotic expression of adamts9 started around germ ring stage and reached peak levels at 3 days post fertilization (dpf) in zebrafish. Germ cell migration completed within 24 hours in wildtype sibling, while a delay in germ cell migration was found at 15 and 24-hours post-fertilization (hpf) in the Adamts9 knockout (KO). However, this delayed effect of Adamts9 KO disappeared at 48 hpf. Our study suggests a conserved function of Adamts9 in germ cell migration among invertebrates and vertebrates. In addition, our results also suggest that Adamts9 is not essential for germ cell migration as reported in C. elegans, possibly due to expansion of Adamts family members and compensatory roles from another metalloproteinase in vertebrates. Further studies are required in order to elucidate the functions and mechanisms of metalloproteinases in germ cell migration and gonad formation in vertebrates.
Application of Data Linkage Techniques to Pacific Northwest Commercial Fishing Injury and Fatality Data&nbsp;
Authors: Nahorniak Jasmine , Bovbjerg Viktor , Case Samantha , Kincl Laurel 
Published: January 2021

Abstract
BackgroundCommercial fishing consistently has among the highest workforce injury and fatality rates in the United States. Data related to commercial fishing incidents are routinely collected by multiple organizations which do not currently coordinate or automatically link data. Each dataset has the potential to generate a more complete picture to inform prevention efforts. Our objective was to examine the utility of using statistical data linkage methods to link these datasets in support of incident surveillance and hazard assessment in the commercial fishing industry.MethodsIn this feasibility study, we identified true matches and discrepancies between de-identified datasets using the Python Record Linkage Toolkit. Four commercial fishing datasets from Oregon and Washington were linked: the Commercial Fishing Incident Database, the Vessel Casualty Database, the Nonfatal Injuries Database, and the Oregon Trauma Registry. The datasets each covered different date ranges within 2000 - 2017, containing 458, 524, 184, and 11 cases respectively. Several data linkage classifiers were evaluated.ResultsThe Naïve-Bayes classifier returned the highest number of true matches between these small datasets. A total of 41 true matches and 8 close matches were identified, of which 29 were determined to be duplicates. In addition, linkage highlighted 4 records that were not commercial fishing cases from Oregon and Washington. The optimum match parameters were the date, state, vessel official number, and number of people on board.ConclusionsStatistical data linkage enables accurate, routine matching for small de-identified injury and fatality datasets such as those in commercial fishing. It provides information needed to improve the accuracy of existing data records. It also enables expanding and sharpening details of individual incidents in support of occupational safety research.