bluuebunny/crossref_metadata_2025_split huggingface dataset:
Pengaruh Pengaturan Komposisi Media Tanam Organik Terhadap Pertumbuhan Dan Hasil Tanaman Ubi Jalar ( Ipomoea batatas L.) Dengan Sistem Tabilampot
Authors: Mardiyanto Mohamad Ghufron , Muslikah Siti , Nurhidayati N 
Published: June 2021

Sweet potatoes are an alternative source of carbohydrates that have high nutritional content. Development of sweet potato cultivation methods needs to be done to overcome the decrease of productive agricultural land due to land conversion. This study aimed to test the effect of differences in the composition of the growing media on the growth, yield, and quality of sweet potato. This study was a pot experiment using a Randomized Block Design (RBD) with 7 treatments and 5 replications. The types of treatments tested were P0: 100% soil + inorganic fertilizer; P1: 50% soil + 50% cow manure; P2: soil 50% + vermicompost 50%; P3: soil 50% + biochar rice husk 25%, cow manure 25%, P4: soil 50% + biochar rice husk 25% + vermicompost 25%, P5: soil 50% + cocopeat 25% + cow manure 25%, P6: soil 50% + cocopeat 25% + vermicompost 25%. The research results showed that the composition of the growing medium significantly affected plant growth and yield. The results of the statistical analysis showed that the treatments of P4 and P6 gave the highest growth, while the high yield of fresh weight of tubers per pot were found in the treatments P1, P2, P3, and P4 by 165.59 g, 143.38 g, 171.56 g, and 144.80 g, respectively. The highest number of tubers was found in treatment P6 by 7.66 tubers. The highest yield of dry matter was also found in treatments P1, P2, P3, and P4 by 59.91 g, 51.73 g, 59.02 g, and 48.59 g, respectively. Based on the research results, it can be recommended that the cultivation of sweet potato plants in pots can be carried out using porous growing media in a container that is sufficient and available balanced nutrients for the development of plant tubers.Keywords: Vermicompost, composition of growing media, Sweet Potatoes, Yield, Quality
NILAI ORGANOLEPTIK DAN PH TELUR ITIK ASIN DENGAN PENAMBAHAN SEREI (Cymbopogon citratus) DAN DAUN BAWANG (Allium fistulosum) PADA LEVEL YANG BERBEDA
Authors: Mardin Alpian , Nurhaedah Nurhaedah , Rustam Rasbawati , Novieta I.D. , Fitriani Fitriani 
Published: June 2021

Penelitian ini bertujuan untuk mengetahui penggunaan ekstrak serai dan daun bawang terhadap pH dan nilai organoleptik telur itik atau telur asin, Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan 4 taraf perlakuan yang diulang sebanyak 3 kali yaitu P0= Kontrol, P1= Penambahan ekstrak serai dan daun bawang 10%, P2= Penambahan ekstrak serai dan daun bawang 20%, dan P3= Penambahan ekstrak serai dan daun bawang 30%. Perlakuan penambahan serai dan daun bawang (Cymbopogon Citratus and Allium fistulosum) berpengaruh sangat nyata terhadap nilai organoleptik dan berpengaruh nyata terhadap nilai pH telur itik asin, Nilai rata rata uji organoleptik telur itik asin yaitu aroma 2,18 – 4,20, rasa 2,19 – 4,27, kemasiran 2,51 – 3,43, dan hedonik 2,22 – 4,36 penambahan serai dan daun bawang terhadap nilai pH pada telur itik asin yaitu 6,23 – 6,47. Penambahan serai dan daun bawang 10% menghasilkan nilai pH yang terbaik.. Disarankan kepada peneliti selanjutnya untuk melakukan penelitian lanjutan untuk mengetahui lama masa simpan telur asin untuk dikomersialkan pada masyarakat.
ANALISIS KANDUNGAN MIKROPLASTIK PADA BEBEK (Anas platyrhynchos domesticus) STUDI KAJIAN TINGKAT PENCEMARAN PLASTIK DI TERNAK UNGGAS AIR
Authors: Cahyo Yustian Dwi , Ummah Nahdlatul , Ikbal Mohammad 
Published: June 2021

Sampah plastik merupakan salah satu permasalahan yang dihadapi oleh bangsa Indonesia saat ini. Bukan hanya karena jumlahnya yang melimpah akan tetapi, akibat yang ditimbulkan berupa pencemaran lingkungan khususnya perairan yang mencakup sungai, danau dan laut. Bebek merupakan salah satu unggas air yang berpotensi pertama kali terkena paparan limbah mikroplastik. Sampai saat ini belum ada penelitian tentang kandungan mikroplastik pada saluran pencernaan bebek. Tujuan dari penelitian ini adalah untuk mengetahui adanya kontaminan mikroplastik dan kandungan mikroplastik pada saluran pencernaan unggas air bebek ( Anas platyrhynchos domesticus ) dan menganalisa efek mikroplastik terhadap pola perubahan manajemen pemeliharaan bebek. Metode yang digunakan adalah Metode Survey. Data yang diperoleh dianalisis statistic deskriptif dan t-test untuk memeriksa adanya perbedaan parametric. Pada pengamatan jumlah mikroplastik dilakukan dengan cara ekstraksi komponen biologi menggunakan larutan KOH 10 % pada sampel saluran pencernaan bebek dan pada sampel tanah/pasir menggunakan larutan NaCl pekat. Sampel bebek diambil berdasarkan wilayah pemeliharaannya. Wilayah I (kontrol) adalah wilayah dimana bebek yang dipelihara dengan sistim intensif, Wilayah II bebek dipelihara semi intensif di lingkungan dekat persawahan, Wilayah III Bebek dipelihara di lingkungan padat penduduknya. Hasil dari penelitian ini untuk sampel tanah/pasir pada wilayah I rata-rata jumlah mikroplastik (partikel/ml) 23,33, wilyah II : 67,78 dan wilayah III : 80. Jumlah rata-rata mikroplastik pada saluran pencernaan bebek (partikel/ bebek) pada wilayah I : 2,7, II : 6,7 dan III : 5,3. Kesimpulan adalah jumlah mikroplastik terbanyak diperoleh pada tanah di wilayah padat penduduknya. Pada kontrol masih ditemukan mikroplastik pada saluran pencernaan bebek.
ДЕЯТЕЛЬНОСТЬ МЕСТНЫХ ВЛАСТЕЙ ПО БОРЬБЕ С ЭПИДЕМИЯМИ ВО ВТОРОЙ ПОЛОВИНЕ XIX – НАЧАЛЕ XX ВЕКА (на материалах Костромской губернии)*
Authors: Белов Андрей Михайлович , Галинская Оксана Юрьевна 
Published: June 2021

После отмены крепостного права приток населения в города, в т.ч. в Кострому, привел к проблемам перенаселенности губернского центра и несоответствия существующей инфраструктуры (жилищной, транспортной, коммунальной) изменившейся численности жителей. Данная ситуация способствовала взрывному росту и распространению в Костроме заразных болезней, в том числе, массовых эпидемий холеры, тифа, оспы, скарлатины, следствием которых стала повышенная смертность среди костромичей, приводящая в некоторые годы к убыли населения губернского города.
В статье на материалах Костромской губернии, прежде всего, губернского центра, рассматривается общая эпидемиологическая обстановка с 70-х гг. XIX века до начала XX века, а также основные направления совместной деятельности губернского правления и городских органов общественного управления по снижению эпидемиологической напряженности и профилактике массовых заболеваний в период капиталистической модернизации. В статье приведены статистические данные по Костромской губернии и губернскому центру, использованы отчеты Медицинского департамента Министерства внутренних дел за указанный период, материалы периодической печати, показана региональная специфика решения возникших проблем, учитывая, что Костромская губерния относилась к числу наиболее развитых промышленных губернии Российской Империи.
After the peasant reform of 1861, the growth of the urban population in Kostroma led to the problems of overpopulation in the provincial center. The existing infrastructure did not match (housing, transport, utilities) the changed population. This contributed to the sharp growth and spread of infectious diseases among the population of Kostroma, including massive epidemics of cholera, typhoid, smallpox, scarlet fever. The result was an increased mortality rate among the population. The article, based on materials Kostroma province, primarily the provincial center, examines the general epidemiological situation from the 70s of the 19th century to the beginning of the 20th century. The main directions of joint activities of the provincial government and city public administration bodies to reduce epidemiological tension and prevent mass diseases during the period of modernization are investigated. The article provides statistical data on the Kostroma province and the provincial center, uses the reports of the Medical Department of the Ministry of Internal Affairs for the specified period, materials from periodicals, shows the regional specifics of solving the problems that have arisen, given that the Kostroma province was one of the most developed industrial provinces of the Russian Empire.
Mercury Export From Freshwater to Estuary: Carbocentric Science Elucidates the Fate of a Toxic Compound in Aquatic Boreal Environments
Authors: Demarty Maud , Bilodeau François , Tremblay Alain 
Published: June 2021

The chemistry of mercury in freshwater systems, particularly man-made reservoirs, has received a great deal of attention owing to the high toxicity of the most common organic form, methylmercury. Although methylmercury bioaccumulation in reservoirs and natural lakes has been extensively studied at all latitudes, the fate of the different forms of mercury (total vs. dissolved; organic vs. inorganic) along the entire river-estuary continuum is less well documented. In fact, the difficulty of integrating the numerous parameters involved in mercury speciation in such large study areas, combined with the technical difficulties in sampling and analyzing mercury, have undoubtedly hindered advances in the field. At the same time, carbocentric science has grown exponentially in the last 25 years, and the common fate of carbon and mercury in freshwater has become increasingly clear with time. This literature review, by presenting the knowledge acquired in these two fields, aims to better understand the extent of mercury export from boreal inland waters to estuaries and to investigate the possible downstream ecotoxicological impact of reservoir creation on mercury bioavailability to estuarine food webs and local communities.
Identifying ceRNA Networks Associated With the Susceptibility and Persistence of Atrial Fibrillation Through Weighted Gene Co-Expression Network Analysis
Authors: Liu Yaozhong , Liu Na , Bai Fan , Liu Qiming 
Published: June 2021

Background: Atrial fibrillation (AF) is the most common arrhythmia. We aimed to construct competing endogenous RNA (ceRNA) networks associated with the susceptibility and persistence of AF by applying the weighted gene co-expression network analysis (WGCNA) and prioritize key genes using the random walk with restart on multiplex networks (RWR-M) algorithm.Methods: RNA sequencing results from 235 left atrial appendage samples were downloaded from the GEO database. The top 5,000 lncRNAs/mRNAs with the highest variance were used to construct a gene co-expression network using the WGCNA method. AF susceptibility- or persistence-associated modules were identified by correlating the module eigengene with the atrial rhythm phenotype. Using a module-specific manner, ceRNA pairs of lncRNA–mRNA were predicted. The RWR-M algorithm was applied to calculate the proximity between lncRNAs and known AF protein-coding genes. Random forest classifiers, based on the expression value of key lncRNA-associated ceRNA pairs, were constructed and validated against an independent data set.Results: From the 21 identified modules, magenta and tan modules were associated with AF susceptibility, whereas turquoise and yellow modules were associated with AF persistence. ceRNA networks in magenta and tan modules were primarily involved in the inflammatory process, whereas ceRNA networks in turquoise and yellow modules were primarily associated with electrical remodeling. A total of 106 previously identified AF-associated protein-coding genes were found in the ceRNA networks, including 16 that were previously implicated in the genome-wide association study. Myocardial infarction–associated transcript (MIAT) and LINC00964 were prioritized as key lncRNAs through RWR-M. The classifiers based on their associated ceRNA pairs were able to distinguish AF from sinus rhythm with respective AUC values of 0.810 and 0.940 in the training set and 0.870 and 0.922 in the independent test set. The AF-related single-nucleotide polymorphism rs35006907 was found in the intronic region of LINC00964 and negatively regulated the LINC00964 expression.Conclusion: Our study constructed AF susceptibility- and persistence-associated ceRNA networks, linked genetics with epigenetics, identified MIAT and LINC00964 as key lncRNAs, and constructed random forest classifiers based on their associated ceRNA pairs. These results will help us to better understand the mechanisms underlying AF from the ceRNA perspective and provide candidate therapeutic and diagnostic tools.
Identification of Aging-Related Genes Associated With Clinical and Prognostic Features of Hepatocellular Carcinoma
Authors: Chen Xingte , Wang Lei , Hong Liang , Su Zhixiong , Zhong Xiaohong , Zhou Han , Zhang Xueqing , Wu Junxin , Shao Lingdong 
Published: June 2021

Background: Aging is a well-studied concept, but no studies have comprehensively analyzed the association between aging-related genes (AGs) and hepatocellular carcinoma (HCC) prognosis.Methods: Gene candidates were selected from differentially expressed genes and prognostic genes in The Cancer Genome Atlas (TCGA) database. A gene risk score for overall survival prediction was established using the least absolute shrinkage and selection operator (LASSO) regression analysis, and this was validated using data from the International Cancer Genome Consortium (ICGC) database. Functional analysis was conducted using gene ontology enrichment, Kyoto Encyclopedia of Genes and Genomes analysis, gene set enrichment analysis, and immune microenvironment and tumor stemness analyses.Results: Initially, 72 AGs from the TCGA database were screened as differentially expressed between normal and tumor tissues and as genes associated with HCC prognosis. Then, seven AGs (POLA1, CDK1, SOCS2, HDAC1, MAPT, RAE1, and EEF1E1) were identified using the LASSO regression analysis. The seven AGs were used to develop a risk score in the training set, and the risk was validated to have a significant prognostic value in the ICGC set (p &lt; 0.05). Patients with high risk scores had lower tumor differentiation, higher stage, and worse prognosis (all p &lt; 0.05). Multivariate Cox regression analyses also confirmed that the risk score was an independent prognostic factor for HCC in both the TCGA and ICGC sets (all p &lt; 0.05). Further analysis showed that a high risk score was correlated with the downregulation of metabolism and tumor immunity.Conclusion: The risk score predicts HCC prognosis and could thus be used as a biomarker not only for predicting HCC prognosis but also for deciding on treatment.
A 5-Genomic Mutation Signature Can Predict the Survival for Patients With NSCLC Receiving Atezolizumab
Authors: Lin Jiamao , Wang Xiaohui , Zhang Chenyue , Bu Shuai , Zhao Chenglong , Wang Haiyong 
Published: June 2021

BackgroundAt present, there is a lack of studies focusing on the survival prediction of patients with non-small cell lung cancer (NSCLC) receiving atezolizumab in light of gene mutation characteristic.MethodsPatients with NSCLC receiving atezolizumab from the OAK study were defined as the training group. LASSO Cox regressions were applied to establish the gene mutation signature model to predict the overall survival (OS) rate of the training group. NSCLC patients receiving atezolizumab from the POPLAR study were defined as the testing group to validate the gene mutation signature model. In addition, we compared the OS rate between patients receiving atezolizumab and docetaxel classified according to their risk score based on our gene mutation signature model.ResultsWe successfully established a 5-genomic mutation signature that included CREBBP, KEAP1, RAF1, STK11 and TP53 mutations. We found it was superior to the blood tumor mutation burden (bTMB) score and programmed death ligand 1 (PDL1) expression in the prediction of the OS rate for patients receiving atezolizumab. High-risk patients receiving atezolizumab had a worse OS rate compared with low-risk patients in the training (P = 0.0004) and testing (P = 0.0001) groups. In addition, low-risk patients using atezolizumab had a better OS rate compared with those in use of docetaxel for the training (P &lt;0.0001) and testing groups (P = 0.0095). High-risk patients of the training group (P = 0.0265) using atezolizumab had a better OS rate compared with those using docetaxel. However, the OS difference between atezolizumab and docetaxel was not found in high-risk patients from the testing group (P = 0.6403). Multivariate Cox regression analysis showed that the risk model in light of 5-genomic mutation signature was an independent prognostic factor on OS for patients receiving atezolizumab (P &lt;0.0001). In addition, significant OS benefit could only be found in low-risk patients receiving atezolizumab compared with docetaxel (P &lt;0.0001).ConclusionsThe 5-genomic mutation signature could predict OS benefit for patients with NSCLC receiving atezolizumab. Therefore, the establishment of the 5-genomic mutation panel will guide clinicians to identify optimal patients who could benefit from atezolizumab treatment.
Multiple Country and Breed Genomic Prediction of Tick Resistance in Beef Cattle
Authors: Cardoso Fernando Flores , Matika Oswald , Djikeng Appolinaire , Mapholi Ntanganedzeni , Burrow Heather M. , Yokoo Marcos Jun Iti , Campos Gabriel Soares , Gulias-Gomes Claudia Cristina , Riggio Valentina , Pong-Wong Ricardo , Engle Bailey , Porto-Neto Laercio , Maiwashe Azwihangwisi , Hayes Ben J. 
Published: June 2021

Ticks cause substantial production losses for beef and dairy cattle. Cattle resistance to ticks is one of the most important factors affecting tick control, but largely neglected due to the challenge of phenotyping. In this study, we evaluate the pooling of tick resistance phenotyped reference populations from multi-country beef cattle breeds to assess the possibility of improving host resistance through multi-trait genomic selection. Data consisted of tick counts or scores assessing the number of female ticks at least 4.5 mm length and derived from seven populations, with breed, country, number of records and genotyped/phenotyped animals being respectively: Angus (AN), Brazil, 2,263, 921/1,156, Hereford (HH), Brazil, 6,615, 1,910/2,802, Brangus (BN), Brazil, 2,441, 851/851, Braford (BO), Brazil, 9,523, 3,062/4,095, Tropical Composite (TC), Australia, 229, 229/229, Brahman (BR), Australia, 675, 675/675, and Nguni (NG), South Africa, 490, 490/490. All populations were genotyped using medium density Illumina SNP BeadChips and imputed to a common high-density panel of 332,468 markers. The mean linkage disequilibrium (LD) between adjacent SNPs varied from 0.24 to 0.37 across populations and so was sufficient to allow genomic breeding values (GEBV) prediction. Correlations of LD phase between breeds were higher between composites and their founder breeds (0.81 to 0.95) and lower between NG and the other breeds (0.27 and 0.35). There was wide range of estimated heritability (0.05 and 0.42) and genetic correlation (-0.01 and 0.87) for tick resistance across the studied populations, with the largest genetic correlation observed between BN and BO. Predictive ability was improved under the old-young validation for three of the seven populations using a multi-trait approach compared to a single trait within-population prediction, while whole and partial data GEBV correlations increased in all cases, with relative improvements ranging from 3% for BO to 64% for TC. Moreover, the multi-trait analysis was useful to correct typical over-dispersion of the GEBV. Results from this study indicate that a joint genomic evaluation of AN, HH, BN, BO and BR can be readily implemented to improve tick resistance of these populations using selection on GEBV. For NG and TC additional phenotyping will be required to obtain accurate GEBV.
Association of GTF2I, NFKB1, and TYK2 Regional Polymorphisms With Systemic Sclerosis in a Chinese Han Population
Authors: Liu Chenxi , Yan Songxin , Chen Haizhen , Wu Ziyan , Li Liubing , Cheng Linlin , Li Haolong , Li Yongzhe 
Published: June 2021

ObjectivesSystemic sclerosis (SSc) is an uncommon autoimmune disease that varies with ethnicity. Single nucleotide polymorphisms (SNPs) in the GTFSI, NFKB1, and TYK2 genes have been reported to be associated with SSc in other populations and in individuals with various autoimmune diseases. This study aimed to investigate the association between these SNPs and susceptibility to SSc in a Chinese Han population.MethodA case-control study was performed in 343 patients with SSc and 694 ethnically matched healthy controls. SNPs in GTF2I, NFKB1, and TYK2 were genotyped using a Sequenom MassArray iPLEX system. Association analyses were performed using PLINK v1.90 software.ResultOur study demonstrated that the GTF2I rs117026326 T allele and the GTF2I rs73366469 C allele were strongly associated with patients with SSc (P = 6.97E-10 and P = 1.33E-08, respectively). Patients carrying the GTF2I rs117026326 TT genotype and the GTF2I rs73366469 CC genotype had a strongly increased risk of SSc (P = 6.25E-09 and P = 1.67E-08, respectively), and those carrying the NFKB1 rs1599961 AA genotype had a suggestively significantly increased risk of SSc (P = 0.014). Moreover, rs117026326 and rs73366469 were associated with SSc in different genetic models (additive model, dominant model, and recessive model) (P &lt; 0.05) whereas rs1599961 was associated with SSc in the dominant genetic model but not in the addictive and recessive models (P = 0.0026). TYK2 rs2304256 was not significantly associated with SSc in this study.ConclusionGTF2I rs117026326 and rs73366469 SNPs were strongly associated with SSc in this Chinese Han population. NFKB1 rs1599961 showed a suggestive association with SSc, and no significant association was found between TYK2 rs2304256 and SSc in this Chinese Han population.