bluuebunny/crossref_metadata_2025_split huggingface dataset:
Poor outcome after hematopoietic stem cell transplantation of patients with unclassified inherited bone marrow failure syndromes
Authors: Lim Yeon Jung , Arbiv Omri A. , Kalbfleisch Melanie E. , Klaassen Robert J. , Fernandez Conrad , Rayar Meera , Steele MacGregor , Lipton Jeffrey H. , Cuvelier Geoff , Pastore Yves D. , Silva Mariana , Brossard Josee , Michon Bruno , Abish Sharon , Sinha Roona , Corriveau‐Bourque Catherine , Breakey Vicky R. , Tole Soumitra , Goodyear Lisa , Sung Lillian , Zlateska Bozana , Cada Michaela , Dror Yigal 
Published: December 2021

AbstractClassification of inherited bone marrow failure syndromes (IBMFSs) according to clinical and genetic diagnoses enables proper adjustment of treatment. Unfortunately, 30% of patients enrolled in the Canadian Inherited Marrow Failure Registry (CIMFR) with features suggesting hereditability could not be classified with a specific syndromic diagnosis. We analyzed the outcome of hematopoietic stem cell transplantation (HSCT) in unclassified IBMFSs (uIBMFSs) and the factors associated with outcome. Twenty‐two patients with uIBMFSs and 70 patients with classified IBMFSs underwent HSCT. Five‐year overall survival of uIBMFS patients after HSCT was inferior to that of patients with classified IBMFSs (56% vs 76.5%). The outcome of patients with uIBMFS who received cord blood was significantly lower than that of patients who received other stem cell sources (14.8% vs 90.9%). Engraftment failure was higher among patients with uIBMFS who received cord blood than those who received bone marrow. None of the following factors were significantly associated with poor survival: transfusion load, transplant indication, the intensity of conditioning regimen, human leukocyte antigen‐identical sibling/alternative donor. We suggest that identifying the genetic diagnosis is essential to modulate the transplant procedure including conditioning agents and stem cell sources for better outcome and the standard cord blood transplantation (CBT) should be avoided in uIBMFS.
Characterisation of the oral glucose and sugar tolerance tests and the enteroinsular axis response in healthy adult donkeys
Authors: Mendoza Francisco J. , Buzon‐Cuevas Antonio , Toribio Ramiro E. , Perez‐Ecija Alejandro 
Published: December 2021

SummaryBackgroundInsulin dysregulation (ID) is diagnosed in horses and ponies using oral glucose (OGTT) and oral sugar (OSTT) tolerance tests. The enteroinsular axis plays a major role in postprandial glucose disposal and insulin response in horses, ponies and foals. The insulin and incretin response to oral carbohydrate challenges has not been characterised in donkeys.Objectives(a) To characterise OGTT and OSTT, and (b) to assess the plasma incretin response to OGTT and OSTT in healthy donkeys.Study designIn vivo experiments.MethodsSix healthy adult female Andalusian donkeys were challenged with OGTT (1 g/kg glucose, 20% solution by nasogastric tube) and OSTT (0.45 mL/kg corn syrup orally by syringe) with a 1‐week washout. Blood samples were collected for glucose (spectrophotometry), insulin (radioimmunoassay), glucose‐dependent insulinotropic polypeptide (GIP, ELISA) and active glucagon‐like peptide‐1 (aGLP‐1, ELISA) determination over 6 hours. Curves were analysed and proxies calculated.ResultsGlucose and insulin concentrations peaked at 180 minutes in OGTT, but at 300 and 150 minutes in OSTT, respectively. Plasma GIP concentrations increased in the OGTT and OSTT (peaked at 180 and 360 minutes, respectively), but aGLP‐1 increased only in OGTT (240 minutes).Main limitationsSingle breed, narrow age and sample, diet, season and not having donkeys with evidence of ID to provide clinical validation.ConclusionsDonkeys have a functional enteroinsular axis that is activated by enteral carbohydrates. Donkeys have evident endocrine differences with horses, supporting the validation of the OSTT and OGTT to assess insulin sensitivity in this species to avoid extrapolation from horses.
Micro‐X‐ray fluorescence image analysis of otoliths to distinguish between wild‐born and stocked river‐spawning whitefish captured in the Baltic Sea
Authors: Finnäs Viktor , Lill Jan‐Olof , Saarinen Timo , Lindqvist Christer , Heimbrand Yvette , Jokikokko Erkki , Hägerstrand Henry 
Published: December 2021

AbstractStrontium concentrations are low in fresh waters compared to seawaters. Therefore, wild‐born river‐spawning and stocked freshwater‐reared whitefish Coregonus lavaretus L. display regions with low concentrations of strontium in the centre of their otoliths. Strontium in otoliths from wild‐born river‐spawning whitefish ascending the River Tornionjoki, river‐spawning whitefish stocked as one‐summer‐old fingerlings caught ascending the River Kemijoki, and sea‐spawning whitefish caught near the Åland Islands was mapped using µ‐XRF. The strontium‐depleted regions in the centre of the whitefish otoliths, measured using ImageJ, had mean sizes of 0.18 ± 0.2 mm2 (River Tornionjoki) and 2.3 ± 0.3 mm2 (River Kemijoki), whereas the otoliths from whitefish caught at sea lacked a strontium‐depleted region altogether. Measurement of the area of the strontium‐depleted region in whitefish otoliths provides a convenient method with which to differentiate between whitefish of different provenance and to determine the origins of whitefish in mixed catches.
Identification and characterization of novel proteins associated with CHD4
Authors: Sakaguchi Chihiro , Ichihara Kazuya , Nita Akihiro , Katayama Yuta , Nakayama Keiichi I. 
Published: December 2021

AbstractThe CHD (chromodomain helicase DNA binding protein) family consists of nine chromatin remodeling factors that alter chromatin structure in an ATP‐dependent manner. CHD4 contributes to the regulation of various cellular activities and processes including development through interaction with multiple proteins including formation of the NuRD (nucleosome remodeling and deacetylase activity) complex. Functions of CHD4 that appear not to be mediated by the NuRD complex or other known interactors have also been identified, however, suggesting the existence of unrecognized proteins that also associate with CHD4. We here generated HeLa‐S3 and HEK293T cells with a knock‐in allele for FLAG epitope‐tagged CHD4 and used these cells to identify proteins that bind to CHD4 with the use of immunoprecipitation followed by liquid chromatography and tandem mass spectrometry. LCORL (ligand‐dependent nuclear receptor corepressor like) and NOL4L (nucleolar protein 4 like) were reproducibly identified as novel CHD4 interactors. Furthermore, RNA‐sequencing analysis of HEK293T cells depleted of CHD4, LCORL, or NOL4L revealed consistent up‐regulation of genes related to the Notch signaling pathway. Our results thus suggest that both LCORL and NOL4L may cooperate with CHD4 to suppress the Notch pathway in mammalian cells.
The cell type‐specific ER membrane protein UGS148 is not essential in mice
Authors: Takahashi Osamu , Tanahashi Mayuko , Yokoi Saori , Kaneko Mari , Yanaka Kaori , Nakagawa Shinichi , Maita Hiroshi 
Published: December 2021

AbstractGenomes of higher eukaryotes encode many uncharacterized proteins, and the functions of these proteins cannot be predicted from the primary sequences due to a lack of conserved functional domains. In this study, we focused on a poorly characterized protein UGS148 that is highly expressed in a specialized cell type called tanycytes that line the ventral wall of the third ventricle in the hypothalamus. Immunostaining of UGS148 revealed the fine morphology of tanycytes with highly branched apical ER membranes. Immunoprecipitation revealed that UGS148 associated with mitochondrial ATPase at least in vitro, and ER and mitochondrial signals occasionally overlapped in tanycytes. Mutant mice lacking UGS148 did not exhibit overt phenotypes, suggesting that UGS148 was not essential in mice reared under normal laboratory conditions. We also found that RNA probes that were predicted to uniquely detect UGS148 mRNA cross‐reacted with uncharacterized RNAs, highlighting the importance of experimental validation of the specificity of probes during the hybridization‐based study of RNA localization.
Comparison of the effect of mothers and students’ education on the promotion of oral health behaviours in female students, using the health belief model
Authors: Ashoori Fatemeh , Karimi Masoud , Seif Mozhgan 
Published: December 2021

AbstractBackgroundOral diseases are considered an important health challenge around the world, especially among children. This study aimed to compare the effects of mothers and students’ education on female students’ oral health behaviours.MethodsThis three‐arm randomized controlled educational trial was conducted on 261 fifth‐ and sixth‐grade female students. The participants were selected and divided into two intervention groups (students’ mothers and students) and a control group through simple random sampling. The intervention included four 60‐min and two 90‐min education sessions for the students and their mothers, respectively. The data were analysed using the SPSS 22 software.ResultsThe results revealed a significant increase in the mean scores of knowledge (p < 0.001), self‐efficacy (p = 0.038), and cues to action (p < 0.001) in the students’ intervention group. However, in the students‐mothers’ group the mean score of knowledge significantly increased (p < 0.001), and perceived barriers significantly decreased (p = 0.001). After the intervention, a significant difference was observed among the three study groups regarding the mean scores of knowledge (p < 0.001), perceived susceptibility (p = 0.005), perceived severity (p = 0.005), perceived benefits (p = 0.011), cues to action (p < 0.001) and brushing and flossing behaviours (p < 0.001).ConclusionIn this study, the mothers’ education did not have any additional effects on the students’ education. It seems that, in underprivileged communities where mothers had a low level of education, it might be better for oral health education to focus more on the students.
Effect of dental caries management using ‘CAMBRA‐kids’ mobile application for children under 5 years old
Authors: Yeo An‐Na , Lee Su‐Young 
Published: December 2021

AbstractObjectiveTo determine effects of dental caries management using ‘CAMBRA‐kids’ mobile application for children under 5 years old for a period of a year.MethodsThis study was conducted on 119 preschoolers and parents. Parents downloaded ‘CAMBRA‐kids’ mobile application and entered risk factors and protective factors for children. Clinician entered disease indicators after clinical examination of children. Based on the input, the caries risk group was automatically determined by the ‘CAMBRA‐kids’ application. According to the caries risk level, caries management was conducted for 12 months according to guidelines.ResultsChildren's caries risk level changed after conducting caries management for 1 year. In the change of CRA (Caries risk assessment) by factor, risk factors decreased in all risk groups, whereas protective factors increased in all risk groups. Disease indicators increased after 12 months in the extreme high‐risk group and the high‐risk group, but decreased in the low‐risk group.ConclusionThis study evaluated the effect of systematic dental caries management using ‘CAMBRA‐kids’ mobile application for preschool children. As a result, dental caries management had effects on children, especially for the extreme high‐risk group and the high‐risk group. Thus, it is expected to be used in a variety of areas for caries management of preschoolers.
Epidemiology of rosacea in a population‐based study of 161,269 German employees
Authors: Hilbring Caroline , Augustin Matthias , Kirsten Natalia , Mohr Nicole 
Published: December 2021

AbstractBackgroundRosacea is a common chronic skin condition, but data on its epidemiology and related comorbidities are scarce.ObjectivesTo analyze the prevalence and associated cutaneous comorbidities of rosacea in Germany.MethodsVoluntary dermatological full‐body examinations were conducted between 2001 and 2016 in more than 500 German companies by experienced dermatologists and documented electronically. Point‐prevalence rates were calculated, and associations were tested with chi‐squared tests and logistic regression analysis.ResultsA total of 161,269 participants (mean age was 43.2 ± 10.9 years; 55.5% male) were included; 2.1% had rosacea (men: 2.1%, women 2.1%, mean age 50.7 ± 9.3 years). The prevalence of rosacea increased significantly with age (16–29 years: 0.3%; 30–39 years: 0.9%; 40–49 years: 2.0%; 50–59 years: 3.5%; 60–70 years: 5.7%). Furthermore, there was a significant decreasing prevalence from skin type I toward type IV (skin type I: 3.2%; II: 2.2%; III: 1.5%; IV: 0.4%). The most frequent dermatological comorbidities were: telangiectasia (OR = 2.5), folliculitis (OR = 1.8), seborrheic dermatitis (OR = 1.6), acne (OR = 1.6), tinea pedis (OR = 1.4), psoriasis (OR = 1.4), spider veins (OR = 1.1), and hemangioma (OR = 1.1).ConclusionsRosacea is a common skin condition that is most prevalent above the age of 65 years. Rosacea patients have an increased risk for associated comorbidities. Therefore, the diagnostic and therapeutic process for rosacea patients must ensure an integrated, complete dermatological approach in terms of medical care.
Prospective associations between pain at multiple sites and falls among community‐dwelling older Australians
Authors: Balogun Saliu A. , Srikanth Velandai , Leeuw Guusje van der , Callisaya Michele L. 
Published: December 2021

AbstractBackgroundPain at multiple sites is prevalent among older people. However, studies investigating the relationship between pain and falls focus largely on single‐site pain.AimTo examine the association between pain at multiple sites and falls among community‐dwelling older Australians.MethodsParticipants aged >60 years were randomly selected from the electoral roll. Falls were recorded prospectively over 12 months. Pain at multiple sites was assessed using a questionnaire. The total number of painful sites was calculated. Widespread pain was defined as pain in the upper limb, lower limb and in the axial skeletal region, with moderate to severe pain in at least one region. Log multinomial regression, with adjustment for confounders, was used to estimate whether widespread pain or number of painful sites increased the risk of a single (one fall) and multiple falls (≥2 falls).ResultsThere were 299 participants (43% women; mean age 72 ± 7.0 years). The mean (standard deviation) of falls was 0.8 ± 1.5. The frequencies of single and multiple falls were 23% (n = 68) and 16% (n = 49) respectively. A higher number of painful sites was associated with an elevated risk of a single fall (relative risk (RR) = 1.08; 95% confidence interval (CI): 0.96–1.27) and multiple falls (RR = 1.20; 95% CI: 1.02–1.41). However, the risk of multiple, but not single fall was substantial and statistically significant. Furthermore, compared with those without pain, individuals with widespread pain had an elevated risk of single (RR = 1.87; 95% CI: 0.80–5.09) and multiple falls (RR = 3.94; 95% CI: 1.15–13.51). However, the magnitude of effects of single fall was smaller and not statistically significant.ConclusionsFalls risk should be ascertained for older people with pain, irrespective of whether they present with a single or multiple sites pain. Nevertheless, older adults with multisite pain may require additional attention as they appear to have a significantly elevated risk of multiple falls.
Myocardial perfusion imaging failed to improve patient risk classification compared with the revised cardiac risk index for early cardiac complications after major non‐cardiac surgery
Authors: Yao Yao , Quirk Thomas , French Matthew , Dharmalingam Ashok , Collins Nicholas 
Published: December 2021

AbstractBackgroundMyocardial perfusion imaging (MPI) is frequently used for cardiac risk assessment before major non‐cardiac surgery, but its ability to improve patient risk classification beyond simple clinical assessment is unknown.AimTo explore the prognostic utility of MPI above a simple clinical risk calculator, the revised cardiac risk index (RCRI).MethodsA retrospective cohort study of at‐risk patients who underwent MPI before major non‐cardiac surgery in a tertiary hospital was conducted. Major adverse cardiac events (MACE) was defined as any myocardial infarction, acute pulmonary oedema, ventricular arrhythmia or cardiac death within 30 days of surgery. We analysed the predictive value of MPI for MACE using multivariable logistic regression and categorical net reclassification index.ResultsMACE occurred in 47 (7.4%) cases from 635 surgical procedures in 629 patients. MPI‐identified medium or large‐sized reversible perfusion defects (P = 0.02; odds ratio 2.9; 95% confidence interval 1.1–7.1) and RCRI score two or more (P = 0.03; odds ratio 2.3; 95% confidence interval 1.1–4.8) were significantly associated with MACE after adjusting for age, coronary revascularisation, surgical priority, need for general anaesthesia, left ventricular ejection fraction (LVEF) and fixed perfusion defects. MPI risk factors (LVEF, reversible perfusion and fixed perfusion defects) did not improve risk classification above baseline risk factors (age, RCRI and surgical priority).ConclusionMPI risk factors are weak predictors for early cardiac complications after major non‐cardiac surgery and failed to improve patient risk classification beyond essential assessment using age, RCRI and surgical priority. Clinicians should consider alternative risk assessment strategies because of MPI's poor prognostic utility and its associated time and financial costs.
Direct percutaneous endoscopic gastrostomy for nutritional support in patients with aerodigestive tract cancers
Authors: Yang Linda S. , Taylor Andrew C. F. , Thompson Alexander J. , Desmond Paul V. , Holt Bronte A. 
Published: December 2021

AbstractBackgroundConventional pull‐through percutaneous endoscopic gastrostomy (PEG) risks infection and tumour implantation in head and neck cancers. Endoscopically inserted direct gastrostomy has lower rates of complications but is underutilised.AimsTo describe the endoscopic steps for direct gastrostomy insertion and review our single‐centre experience to assess the technical feasibility and safety.MethodsPatients who underwent endoscopic direct gastrostomy insertion between December 2016 and June 2021 were included. A 24Fr introducer kit for gastrostomy feeding tube (Avanos Healthcare, Australia) was used. Patient and tumour characteristics, procedural data and 30‐day outcomes were recorded.ResultsThirty patients underwent direct PEG insertion (mean age 64 years and 24 male). All were planned for or currently undergoing radiotherapy. Twenty‐six (87%) of 30 cases were performed under conscious sedation over a median procedure time of 21 min (interquartile range 11 min). No tumour seeding was seen, and one case of PEG‐site infection was observed.ConclusionsDirect PEG is safe and effective and should be considered for patients with aerodigestive tract cancer in need of nutritional support.
Sustainable business practices in manufacturing SMEs: The mediating effect of dynamic capabilities
Authors: Yacob Peter , Peter Darren , Chin Khor Saw 
Published: December 2021

AbstractThis study aims to assess the economic, social, and environmental sustainability of Malaysian manufacturing small and medium‐sized enterprises (SMEs) and the role of dynamic capacities in promoting sustainable business practices. The data was gathered through a survey completed by 216 Malaysian manufacturing SMEs with an SEM‐AMOS nested model comparison analysis. The findings revealed that economic, social, and environmental dimensions are related to sustainable business practices. Besides, firms' dynamic capabilities fully mediate the association between economic, social, and environmental dimensions in Malaysian manufacturing SMEs. This study intended to assist academics, practitioners, policymakers, and other sectors in establishing sustainability to achieve the Sustainable Development Goal 9: Industry, Innovation, and Infrastructure (SDG 9).
Synthesis of SiBNC‐Al ceramics with different aluminum contents via polymer‐derived method
Authors: Tang Zicheng , Wang Shaofei , Tusiime Rogers , Hu Jidong , Chen Haikun , Zhang Yifan , Zhang Hui , Liu Yong 
Published: December 2021

AbstractThis study reports the synthesis of three types of aluminium (Al)‐modified polyborosilazane ceramic precursors (PBSAZ) from C8H19Al/HSiCl3/HMDZ/BCl3 and their thermal conversion to SiBNC‐Al ceramics at 1000°C. FT‐IR, nuclear magnetic resonance (NMR), X‐ray photoelectron spectroscopy (XPS), and thermogravimetric analysis (TGA) are used to characterize the structures and properties of the PBSAZ. PBSAZ is found to be built up of the Si─N─B framework, with Al successfully introduced into the ceramic network structures. The ceramic yield is 63.5 wt.% for the Al‐poor polymers (PBSAZ‐5) and 65.1 wt.% for the Al‐rich polymers (PBSAZ‐1). The high‐temperature cracking behavior and the crystal phase structures of the ceramics were characterized by XRD and Raman, which revealed that SiBNC‐Al ceramics contained Si4N3, SiC, and AlN (+SiC) crystals after heat treatment at 1600°C. The oxidation behavior of SiBNC and SiBNC‐Al ceramics at 1500°C shows that the introduction of Al improves the quality of the oxide layer, effectively suppresses the oxide layer cracking phenomenon, and reduces gas bubble generation.
Prevalence and conservation of ebp genes in Enterococcus faecalis originated from animals
Authors: Dong Jiajun , Zhang Liujun , He Hengxu , Jin Yue , Wang Yabin , Chen Liying 
Published: December 2021

Abstract

Aims
The study aimed to investigate the prevalence and conservation of endocarditis and biofilm-associated pili (ebp) genes in Enterococcus faecalis originated from animals and the potential of developing Ebp into serological diagnostic and vaccine targets.

Methods and Results
In this work, we investigated the prevalence and conservation of ebp genes in 116 strains of E. faecalis originated from animals by using PCR and sequencing methods. The results demonstrated the presence of ebp genes (ebpA, ebpB and ebpC) in all 116 strains of E. faecalis, and their amino acid homology ranges from 96.6% to 100.0%. Moreover, the phylogenetic analysis of ebp genes in all 164 E. faecalis strains (including 48 reference strains) revealed that ebp genes show no significant correlation with species origins and regions of E. faecalis, indicating that ebp genes are conserved features in E. faecalis, even though it evolved under environmental pressures from various regions and origins. Given that EbpA1 as a part of the adhesion protein EbpA has immunogenicity, we further determined whether amino acid mutations have effects on the function and 3D structure of EbpA1. The results showed that two of the 26 mutations, at amino acids positions 178 and 387, had deleterious effects on the biological function of EbpA1 protein, while all mutations had no effect on the 3D structure or binding pockets of EbpA1 protein.

Conclusions
This study suggests that ebp genes are prevalent and conserved in E. faecalis originated from diverse animal origins and regions. EbpA1 could be a potential target for serological diagnosis and vaccine development to prevent E. faecalis infection.

Significance and Impact of Study
The current study provides data to support further research on Ebp as a serological diagnostic and vaccine target against E. faecalis infection.