bluuebunny/crossref_metadata_2025_split huggingface dataset:
Sp1-Induced Upregulation of LBX2-AS1 Aggravates The Progression of Glioblastoma By Targeting The miR-491-5p/LIF Axis
Authors: Li Wentao , Soufiany Ismatullah , Lyu Xiao , Zhao Lin , Lu Chenfei , Wei Yutian , Shi Zhumei , You Yongping 
Published: March 2021

Abstract
Background: Mounting evidences have shown the importance of lncRNAs in tumorigenesis and cancer progression. LBX2-AS1 is an oncogenic lncRNA that has been found abnormally expressed in gastric cancer and lung cancer samples. Nevertheless, the biological function of LBX2-AS1 in glioblastoma (GBM) and potential molecular mechanism are largely unclear. Methods: Relative levels of LBX2-AS1 in GBM samples and cell lines were detected by qRT-PCR and FISH. In vivo and in vitro regulatory effects of LBX2-AS1 on cell proliferation, epithelial-to-mesenchymal transition (EMT) and angiogenesis in GBM were examined through xenograft models and functional experiments, respectively. The interaction between Sp1 and LBX2-AS1 was assessed by ChIP. Through bioinformatic analyses, dual-luciferase reporter assay, RIP and Western blot, the regulation of LBX2-AS1 and miR-491-5p on the target gene leukemia Inhibitory factor (LIF) was identified. Results: LBX2-AS1 was upregulated in GBM samples and cell lines, and its transcription was promoted by binding to the transcription factor Sp1. As a lncRNA mainly distributed in the cytoplasm, LBX2-AS1 upregulated LIF, and activated the LIF/STAT3 signaling by exerting the miRNA sponge effect on miR-491-5p, thus promoting cell proliferation, EMT and angiogenesis in GBM. Besides, LBX2-AS1 was unfavorable to the progression of glioma and the survival. Conclusion: Upregulated by Sp1, LBX2-AS1 promotes the progression of GBM by targeting the miR-491-5p/LIF axis. It is suggested that LBX2-AS1 may be a novel diagnostic biomarker and therapeutic target of GBM.
Intelligent Fluorescence Image Analysis of Giant Unilamellar Vesicles and Protein Liquid Phase Droplets by Whole Z-stack Analysis
Authors: Lee Il-Hyung , Passaro Sam , Ozturk Selin , Wang Weitian 
Published: March 2021

Abstract
Fluorescence image analysis in biochemical science often involves the complex tasks of identifying samples for analysis and calculating the desired information from the intensity traces. Analyzing giant unilamellar vesicles is one of these tasks. Researchers need to identify many vesicles to statistically analyze the degree of molecular interaction or state of molecular organization on the membranes. This analysis is complicated, requiring a careful manual examination by researchers, so automating the analysis can significantly aid in improving its efficiency and reliability. We developed an intelligent analysis routine based on the 3D information of whole z-stack images. The program identifies the valid vesicles to analyze and calculates the desired data automatically. The program can examine the amount of protein binding on the membranes and determine the state of domain phase separation by calculating the fluorescence intensity trace along the membranes. We also show that the method can easily be applied to similar analyses, such as intensity analysis of phase-separated protein droplets. A deep learning-based classification approach enables the identification of vesicles even from relatively complex samples. We demonstrate that the proposed artificial intelligence-assisted classification can further enhance the accuracy of the analysis close to the performance of manual examination.
Different Methods for Termination of Mid-trimester Pregnancy With Placenta Previa&nbsp;
Authors: Long Qingyun , Wu Shiyao , Du Shuguo , Li Ruyan , Zhao Yun , Tang Fei 
Published: March 2021

Abstract
Objective: Investigate the different methods for termination at mid-trimester in pregnant women with placenta previa. Methods: A retrospective study was conducted on 264 cases for termination at mid-pregnancy in our hospital, and 34 cases with placenta previa were set as the observation group, and 230 cases with normal placenta were set as control group. Among them, the preferred methods of termination at first were Mifepristone combined with Misoprostol/Rivanol in the observation group, and Mifepristone combined with Misoprostol/Rivanol/cervical double balloon (CDB) in the control group. If the volume of prenatal bleeding was up to 100 mL in the observation group, emergency artery embolization (UAE) was implemented to stop bleeding, then CDB plus with curettage were used in order. If it failed to induce in the control group, CDB was used subsequently followed with Misoprostol/Rivanol. Then, all those cases were set as the comprehensive-induce group, and the others were set as the simple-induce group.Results: the average maternal age, the number of gravidity/parities, the rate of cesarean section, the hospitalization days and cost, the induction time, the rate of postpartum hemorrhage, puerperal infection were higher in the observation group than those in the control group(p<0.05). There were 4 cases used UAE+CDB+curettage in the observation group and 6 cases used CDB after Misoprostol/Rivanol in the control group. The duration time of termination, the rate of postpartum hemorrhage and transferring to ICU, hospitalization days and cost in the comprehensive-induce group were significantly higher than those in the simple-induce group (p<0.05). All cases were delivered through vaginal successfully.Conclusion: We should pay more attention to the complications of prenatal bleeding, postpartum hemorrhage, puerperal infection during the induction at mid-trimester in pregnant women with placenta previa. Emergency UAE + CDB +curettage is a good combination method in prenatal hemorrhage of placenta previa during termination, and CDB was a good tool for cervical ripen with immature cervical condition in mid-trimester for induction of labor.
Potassium-magnesium Imbalance Causes Detrimental Effects on Growth, Starch Allocation and Rubisco Activity in Sugarcane Plants&nbsp;
Authors: Garcia Ariani , Crusciol Carlos Alexandre Costa , Rosolem Ciro Antonio , Bossolani João William , Nascimento Carlos Antonio Costa , McCray James Mabry , Reis André Rodrigues dos , Cakmak Ismail 
Published: March 2021

Abstract
The authors have requested that this preprint be removed from Research Square.
The transformation of mesopelagic structure across polar fronts
Authors: Chawarski Julek , Klevjer Thor , Coté David , Maxime Geoffroy 
Published: March 2021

Abstract
The oceans sequester 31% of atmospheric carbon annually 1, but the magnitude of biologically enhanced sequestration is not evenly distributed across the globe 2. Measuring surface primary productivity offers a reasonable proxy for estimating carbon flux into the ocean 3 but entirely misses the processes that affect carbon export to sequestration depths. A high proportion of carbon flux is broken up 4 or respired 5 by organisms at mesopelagic depths (200-1000 m). At low and mid-latitudes, daytime biomass peaks of mesopelagic organisms are clearly present and detectable as sound-scattering layers, and up to 40% of oceanic carbon passes through these layers 6,7. Here we demonstrate that acoustic backscatter, a proxy for abundance of mesopelagic fish and zooplankton decreases by up to 97% across polar fronts, revealing a distinct pelagic structure in high latitude systems. As mesopelagic fish and macrozooplankton comprise a large component of active carbon transport in the upper ocean, this latitudinal reduction in mesopelagic biomass across thermal fronts, in both the Arctic and Antarctic, suggests different carbon flux attenuation schemes exists in ocean twilight zones of polar ecosystem compared to low latitude systems. Rapid ocean warming projected at mesopelagic depths8 will likely disrupt vertical connectivity and alter biogeochemical cycles at high latitudes.
SARS-CoV-2 transmission in an indoor mass-gathering live music event. A randomized clinical trial.
Authors: Llibre Josep M , Revollo Boris , Blanco Ignacio , Soler Pablo , Toro Jessica , Izquierdo-Useros Nuria , Puig Jordi , Puig Xavier , Navarro Valentí , Casañ Cristina , Ruiz Lidia , Perez-Zsolt Daniel , Videla Sebastià , Clotet Bonaventura 
Published: March 2021

Abstract
The banning of mass-gathering indoor events to prevent SARS-CoV-2 spread cause an important impact on local economies. We designed a randomized-controlled open-label trial to assess the effectiveness of a comprehensive preventive intervention for a mass-gathering indoor event (a live concert) based on systematic screening of attendees with antigen-detecting rapid diagnostic tests, use of facial mask, and adequate air ventilation. According to a RT-PCR test performed on day 8 after the event, none of the 465 assistants in the experimental arm (i.e., event attendants) became infected by SARS-CoV-2 (observed incidence 0%; Bayesian estimated incidence 0.14%; 95%CI: 0% to 0.61%) versus 2 out of 495 controls (not entering into the event) (0.31%; 95%CI: 0.04% to 0.73%). No significant differences were observed between the estimated incidence in the two study arms. Our study provides evidence on the safety of indoor mass-gathering events conducted during a COVID-19 outbreak under a comprehensive preventive intervention. (ClinicalTrials.gov number, NCT04668625)
Construction of An Index System of Core Competence Assessment For Infectious Disease Specialist Nurse: A Delphi Study
Authors: Wu Chao , Wu Ping , Li Pei , Cheng Feixia , He Shizhe , Du Yanling , Hongjuan Lang 
Published: March 2021

Abstract
Aim and objective: To establish an index system for the evaluation of infectious disease specialist nurse core competence.Background: An index system for the evaluation of infectious disease specialist nurse core competence has not been established.Design: A two-round Delphi survey sought opinions from experts about the index system for the evaluation of infectious disease specialist nurse core competence.Methods: Based on the methods of literature retrieval, theoretical analysis and qualitative research, the draft of core competence evaluation index system of infectious disease specialist nurses was constructed. A Delphi survey was used for the study of 30 infectious disease experts from 8 provinces and cities around China. A modified recommendation for the Conducting and Reporting of Delphi studies (CREDES) was used to guide this study. A STROBE checklist was used.Results: The evaluation index system of core competence of diseases specialist nurses is composed of 6 primary indicators which includes Nursing Abilities for infectious diseases, Infection Prevention and Control Capabilities, Capabilities in Response to Infectious Disease, Professional Development Capabilities, Communication and Management Capabilities and Professionalism and Humanistic Accomplishment, 16 secondary indicators and 47 tertiary indicators. The authority coefficient, judgment coefficient and familiarity degree of Delphi experts were 0.923, 0.933 and 0.913 respectively.Conclusions: The evaluation index system of core competence of diseases specialist nurses is scientific and reliable. It can be reference for future training and assessment of infectious disease specialist nurses.Relevance to clinical practice: Infectious disease specialist nurses are the main force for infectious disease nursing. Their core competence is related to the quality of infectious disease nursing and treatment. The core competence of the nurses is important for identification of training strategies and can be regarded as reference for nurse assessment and promotion. The construction of the index system is based on the consensus of infectious disease experts, which is helpful to standardize the training strategies and selection standards of infectious disease specialist nurses in the future and meet the needs of clinical infectious disease nursing.
Numerical Implementation of Drop Spin and Tilt Method For Five-Axis Tool Positioning For Tensor Product Surfaces
Authors: Sharma Sandeep Kumar , Duvedi Ravinder Kumar , Bedi Sanjeev , Mann Stephen 
Published: March 2021

Abstract
This paper presents an extension of multi point machining technique, called the Drop Spin and Tilt (DST) method, that spins the tool on two axes, allowing for the generation of multiple contact points at varying distances around the first point of contact. The multiple DST second points of contact were used to manually generate a toolpath with uniform spacing between the two points of contact. The original DST method used a symbolic algebra package to position the tool on a bi-quadratic surface; our extension is a numer- ical solution that allows positioning a toroidal tool on a tensor product Bezier surface. Further, we investigate the spread of possible second points of contact as the tool is spun around these two axes, demonstrating the feasability of using the method to control the machining strip width.
Chiral Phonons in Biocrystals
Authors: Choi Won Jin , Yano Keiichi , Cha Minjeong , Colombari Felippe , Wang Yichun , Lee Sang Hyun , Kruger John , Moura Andre de , Kotov Nicholas A 
Published: March 2021

Abstract
Chiral phonons are concerted mirror-symmetric movements of atomic groups connected by covalent and intermolecular bonds. Finding chiral phonons in biocrystals is fundamentally and technologically important because these lattice vibrations should be highly specific to their short- and long-range organizations. Based on theoretical and experimental data they might be expected but not identified or utilized. Here we show that terahertz chiroptical spectroscopy enables registration and attribution of chiral phonons in microcrystals of numerous amino acids and dipeptides. Theoretical analysis and computer simulations confirm that sharp mirror-symmetric bands observed for left and right enantiomers originate from collective vibrations of biomolecules interconnected by hydrogen bonds into helical chains. Structure-property relationships for strong phonons with rotatory components in biocrystals were also identified. Bladder stones and health supplements display strong spectral signatures of chiral phonons indicating their immediate importance for biomedicine.
Association between healthy lifestyle practices and life purpose among a highly health-literate cohort: a cross-sectional study
Authors: Hirooka Nobutaka , Kusano Takeru , Kinoshita Shunsuke , Aoyagi Ryutaro , Nakamoto Hidetomo 
Published: March 2021

Abstract
Background

The national health promotion program in 21st century in Japan (HJ21) correlates life purpose with disease prevention; facilitating the adoption of healthy lifestyles. However, the influence of clustered healthy lifestyle practices on life purpose, in the context of a national health campaign, remains uninvestigated. Hence, this study assessed the association between such practices and life purpose in line with the HJ21.
Methods

A nationwide cross-sectional survey was given to certified specialists in health management. Participants’ demographic information, lifestyle, and purpose in life using a validated tool were measured. The cohort was median-split into two groups based on their clustered health-related lifestyle score. Health-related lifestyle and purpose were compared between the two groups, and the correlation between health-related lifestyles and purpose in life was measured.
Results

Data from 4,820 participants were analyzed. The higher scoring health-related lifestyle group showed a significantly higher life purpose than the lower group (35.3 vs 31.4; t = 23.6, p < 0.001). The association between the scores of clustered healthy lifestyle practices and life purpose was significant (r = 0.401, p < 0.001). The better health-related lifestyle group achieved a higher life purpose than the lower group. This association between healthy lifestyle practices and life purpose denotes a positive and linear relationship.
Conclusions

The present study suggest that individuals who have a better health-related lifestyle gain a higher sense of life purpose. Healthy lifestyle predicted purpose in life. Examining causal relation between healthy lifestyle and purpose in life may provide more efficient approach for health promotion.
Effect of Oral Vitamin E on Oxidative Stress in Diabetic Kidney Disease：A Protocol of Systematic Review and Meta-Analysis
Authors: Wan Renhong , Miao Pengwei , Ji Yue , Fan Yihua , Liu Xiaohan , Fang Yuxin , Li Xinju 
Published: March 2021

Abstract
BackgroundThe occurrence and progression of diabetic nephropathy is closely related to oxidative stress response. Vitamin E, as a lipid soluble antioxidant, has a long history of use. It may improve oxidative stress response to some extent, but there is no relevant support of evidence-based medicine. The purpose of this study was to systematically evaluate the effect of oral vitamin E on oxidative stress response in patients with diabetic nephropathy.MethodsA computer was used for retrieving data from English database (PubMed, Embase, Web of Science, the Cochrane Library) and Chinese database (CNKI, Wangfang, VIP, CBMDISC) between the creation time to October 2020, a randomized controlled clinical study on oral vitamin E treatment of diabetic nephropathy. Two investigators conducted data extraction and literature quality evaluation for the included studies, and a Meta- analysis on the included articles was conducted using RevMan5.3 software.ConclusionsThis study will summarize up-to-date high-quality RCTs to assess the antioxidant effects of oral vitamin E on diabetic nephropathy. The findings of this study will help to determine whether oral vitamin E is effective and safe in patients with diabetic nephropathy.Systematic review registration: PROSPERO CRD42020213945
Optic Disc Neovascularization in Vogt-Koyanagi-Harada Disease- A Case Series and Literature Review
Authors: Paul Lagan , Shah Shalin , Agarwal Manisha , Singh Shalini 
Published: March 2021

Abstract
BackgroundVogt–Koyanagi–Harada (VKH) disease is an autoimmune disorder which affects numerous organs of the body. We report two cases of Optic Nerve Head neovascularisation (NVD) in diagnosed cases of Vogt Koyanagi Harada(VKH) disease.FindingsCase 1: A 40 years female presented with acute loss of vision in both eyes for 10 days. She had multiple serous detachments in both eyes and diagnosed as acute VKH disease. After treatment, she was observed to have NVD later on OCT Angiography(OCTA). Case 2: A 52 years old man presented with history of sudden blackouts in front of left eye since 4 months. He was a case of chronic VKH and NVD was seen clinically and on OCTA. ConclusionThe exact etiopathogenesis of neovascularization in Harada’s disease is unclear. The low grade inflammations acts as a stimulus and can induce disc neovascularization even in the absence of vascular occlusion. ONH vessels appear to be more susceptible to developing NVD than retinal vessels in presence of chronic inflammation.
Lowered Cognitive Function and the Risk of the First Events of Cardiovascular Diseases: Findings from the Cohort Study in Lithuania
Authors: Luksiene Dalia , Sapranaviciute-Zabazlajeva Laura , Tamosiunas Abdonas , Radisauskas Ricardas , Bobak Martin 
Published: March 2021

Abstract
Background: The purpose of this prospective cohort study was to examine whether the level of cognitive function at the baseline expressed as a cognitive function composite score and score of specific domains predict the risk of first cardiovascular diseases (CVD) events in the middle-aged and older population.Methods: 7,087 participants, men and women aged 45-72 years, were assessed in the baseline survey of the Health Alcohol Psychosocial Factors in Eastern Europe (HAPIEE) study in 2006–2008 in Kaunas city, Lithuania. During 10 years of follow-up, the risk of first non-fatal events of CVD and death from CVD (excluding those participants with a documented history of CVD and/or ischemic heart disease (IHD) diagnosed at baseline survey) was evaluated. Results: During the follow-up, there were 156 deaths from CVD (49 women and 107 men) and 464 first non-fatal CVD events (195 women and 269 men) registered. The total number of first CVD events was 620 (11.5%). After adjustment for sociodemographic factors, biological and lifestyle risk factors, and illnesses, a decrease per 1 standard deviation in different cognitive function scores significantly increased risk of the first event of CVD (immediate verbal recall score - by 17% in men and 32% in women; delayed verbal recall score – by 17% in men and 24% in women; and a composite score of cognitive function – by 15% in men and 29% in women). Kaplan-Meier survival curves for the probability of the first cardiovascular event according to the categories of a composite score of cognitive function, revealed that the lowered cognitive function predicts a higher probability of the events compared to normal cognitive function (p < 0.05).Conclusions: The findings of this follow-up study suggest that men and women with lower cognitive functions have an increased risk for the first event of CVD compared to participants with a higher level of cognitive functions.
Hydrogeochemical characteristics and genesis of Seismic observation wells in Shandong Province
Authors: Su Shujuan , Chen Qifeng , Zou Chunhong , Du Guilin , Wang Heqian , Feng Enguo , Yan Bingdun 
Published: March 2021

Abstract
Hydrogeochemical characteristics, controlling factors, and recharge sources of the seismic observation wells in Shandong Province were investigated by analyzing cation and anion concentrations, hydrogen and oxygen isotopes in well water. A total of 17 water samples in seismic observation wells were collected on April 25-29, 2018. The results show that temperatures of seismic observation wells were in the range of 14.8 to 52.1°C, and the values of δD and δ 18 O ranged from -72.4‰ to -37.9‰ and from -9.4‰ to -4.3‰, respectively. Using C.A. ЩукаЛев’s classification method, the water samples of 17 seismic observation wells were classiﬁed into 7 types: Cl·SO 4 -Na·Ca, SO 4 -Na, Cl-Na, HCO 3 -Na·Ca, HCO 3 -Mg·Na·Ca, HCO 3 -Na and HCO 3 -Mg·Ca·Na. The results indicate that the hydrogeochemical characteristics of 17 seismic observation wells, with a certain spatial distribution pattern, are affected by several factors, such as the tectonic, topography, stratigraphy, hydrology and meteorology. The analyses of ratio coefficients, Schooller diagram, hydrogen and oxygen isotopes compositions, Giggenbach and Gibbs diagram suggest that the atmospheric precipitation is the main recharge source of 17 observation wells. The recharge sources of deep lateral runoff and sedimentation water, moreover, play a significant role in some seismic observation wells. Combined with the amount of precipitation, the distance from recharge areas, the closure degree of observation wells and the stage of water-rock reactions, the development directions of faulting and topography control the directions of groundwater recharge, runoff and discharge, which make hydrogeochemical characteristics represent complex spatial distribution rules.