bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Characterization of Stenotrophomonas maltophilia Isolated from Marine Sponge Producing Salt Tolerant Proteases
Authors: Nurul Sherina Muzaini  , Nurulhuda Mohd Jailani  , Tengku Abdul Hamid Tengku Haziyamin 
Published: September 2021

Protease is an important industrial enzyme and salt tolerant protease in which has desirable properties that could enhance its uses not only in industries, but also in agriculture and environmental. Marine organisms usually harbour halophilic microorganisms which produce salt tolerant protease. In this study, salt tolerant protease producing bacteria from marine sponge were isolated and screened on skim milk marine agar supplemented with different NaCl concentrations (1.5 % w/v). Out of 11 isolates, 8 isolates (S1-1, S1-2, S1-3, S2-1, S2-3, S2-4, S2-5 and S2-6) showed clearing zones with ability to digest casein on the skim milk agar. Morphologically, these strains are gram negative bacilli which grow in yellow colonies and were found to be catalase-positive but oxidase-negative. They are also non-lactose fermenter that produce gelatinase but not α-amylase. The ribosomal 16S rRNA sequencing was used to identify each isolate (Acc. number of S1-1 for MT645770, S1-2 for MT645 771, S1-3 for MT645 772, S2-1 MT645 773, S2-3 MT645 774, S2-4 MT645 775, S2-5 MT645 776 and S2-6 MT645 7767). The 16S rRNA sequences showed that these isolates were highly similar to Stenotrophomonas maltophilia (S2-6, 99.87%) and to a related strain Pseudomonas hibiscicola (S1-1, S1-2, S1-3, S2-1, S2-3, S2-4, S2-5 (97.16-99.9 %). Further proteolytic studies were carried out using skim milk agar with 1.0 %, 2.0 %, 3.0 % and 4.o % (w/v) of NaCl concentrations. All isolates were able to hydrolyze casein which produced clear zones surrounding each colony at 1.0 % and 2.0 % (w/v) salt. However, only isolate S1-5 and S2-6 showed proteolytic activities at 3.0 % (w/v) salt but none of them at 4.0 % (w/v). The ability of these isolates to produce protease which active at higher salt may indicate their potential to be the sources for enzyme with useful properties.
Production of High Quality Planting Materials Through Breeding for Four Important Herbal Species
Authors: Farah Fazwa Md Ariff  , Syafiqah Nabilah Samsul Bahari  , Zunoliza Abdullah  , Ling Sui Kiong  , Masitah Mohd Taini  , Fadhilah Zainudin  , Siti Nur Aisyah Mohd Hashim  , Fadzureena Jamaludin  , Mohd Zaki Abdullah  
Published: September 2021

With the growing interest in these species as a source of new pharmaceutical products and the increasing demand for herbal products in Malaysia, the demand for its raw materials is also increasing. Therefore, initiatives have been taken by Forest Research Institute Malaysia (FRIM) to select and to produce high quality planting materials for future uses. To our knowledge, most of the raw materials used in producing herbal products and its development were being sourced from the wild, with little knowledge on the quality of the raw materials. In order to ensure the quality and sustainability of raw materials in the market, it is important to come out with suitable breeding strategy for the selected species. This paper highlights research by FRIM on collecting, screening, selecting and producing high quality planting materials for the four species based on their growth performance and bioactive compounds. Several research on the production of high quality planting materials for four important medicinal plants has been initiated by FRIM starting from the 11th Malaysian Plan until now. This paper discusses about four selected species namely Chromolaena odorata (kapal terbang), Andrographis paniculata (creat or green chiretta), Beackea frutescens (false ru) and Senna alata (candle bush). These species are found to have a significant benefit to the human’s health. C. odorata (kapal terbang) leaf extract are found to have relatively strong inhibition on platelet-activating factor (PAF) receptor binding in vitro, indicating an anti-inflammatory activity, which is favourable for wound healing. A major bioactive compound in A. paniculata, andrographolide has shown anticancer potential in various research. Whereas, B. frutescens has a potential to be used as anti-gout remedies. Research conducted by FRIM showed that active compound in the leaves and stems of B. frutescens extract are effective in inhibiting uric acid formation and promoting uric acid secretion. Whereas for S. alata, the highest content of major flavonoid glycoside compound, kaempferol-3-O-gentiobioside (K3G), has an anti-inflammatory effect detected in leaf extracts. Germplasm plots for the species were also established in FRIM for future breeding activities. The outputs derived from this study will support the herbal industries in getting quality raw materials in the future. By using high quality plants will also increase the value of pharmaceutical products in the market. It is anticipated that herbal industries and interested party will seek FRIM for high quality seeds and seedlings materials for the development of their products as well as for the establishment of commercial herbal plantation.
Developing Family Satisfaction with Care in Adult Critical Care Public Hospital Terengganu, Malaysia
Authors: Wan Abdul Rahman Wan Nor Aliza , Abdul Karim Othman  , Yuzana Mohd Yusop  , Asyraf Afthanorhan  , Hasnah Zani  , Siti Suhana Zakaria  
Published: September 2021

In admissions to the intensive care unit (ICU), there is a high possibility of a life-threatening condition and possible emotional distress for family members. When the family is distressed and hospitalized, a significant level of stress and anxiety will be generated among family members, thereby decreasing their ability to make responsible decisions. As a result, the family members need full and up-to-date details, helping them to retain hope, and this contributes to lower stress levels. While there is growing evidence of the effectiveness of shared decision-making for family members who are directly involved in decisions, particularly regarding shared decision-making in the Malaysian context, there is less evidence that supported decisions help overall outcome. This study aims to developing the family satisfaction with decision making in the Intensive Care Unit (FS-ICU)-33 Malay language version of family member’s satisfaction with care and decision making during their stay at the intensive care units. A quantitative, cross-sectional validation study and purposive sampling was conducted from 1st November 2017 and 10 October 2018 to January 2020 among 208 of family members. The family members of the ICU patients involved in this study had an excellent satisfaction level with service care. Higher satisfaction in ICU care resulting in higher decision-making satisfaction and vice versa.
Food Value Chain, Sustainable Intensification and Food Security in Malaysia
Authors: Zainol Fakhrul Anwar , Aik Chua Kim 
Published: September 2021

Malaysian seed industry is at an early phase of development due to a lack of participation and an undeveloped seed industry, hence, previous research on this area is very limited. This research adopts Yin’s case study approach which have been applied in a wide range of scholarly studies to the context of agribusiness research project . The findings of this study pertain to the components in the food value chain that influence food security which reveal that the respondents perceive food security is built on four important dimensions; namely, accessibility, availability, stability and utilization. Based on the respondents’ view, Malaysia’s food security status is not at the most healthy level since respondents noted the import bill remains at an all-time high causing an urgent need to address this problem. Most feel an increase in agriculture productivity is the most likely recommended option. Agriculture productivity depends on agriculture input of which, the seed is the fundamental component of the food value chain, hence, respondents suggested intensifying the seed industry to increase productivity.
Kualitas Kontraktor Dalam Melaksanakan Proyek Rehabilitasi dan Pemeliharaan Jaringan Irigasi Pada DPU Kabupaten Ende
Authors: Silva Yustina Rere Da , Nisanson Marselinus Y. , Ester Elim 
Published: September 2021

Pelaksanaan pekerjaan proyek rehabilitasi/pemeliharaan jaringan irigasi pada Bidang Sumber Daya Air Dinas Pekerjaan Umum dan Penataan Ruang Kabupaten Ende Tahun Anggaran 2019 dilaksanakan oleh CV. Berdikari, CV. Nusa Alam, CV. Muncul Baru, dan CV. Yordan mengalami keterbatasan antara lain: tenaga kerja kurang memiliki pemahaman terhadap spesifikasi teknik dan gambar kerja, tidak melakukan pengajuan uang muka, dan kesulitan metode kerja yang diisyaratkan. Penelitian ini bertujuan untuk mengetahui kualitas kontraktor dan hubungan antara aspek: sumber daya manusia, keuangan/finansial, dan aspek metode pelaksanaan dengan metode deskriptif kuantitatif. Data penelitian diperoleh melalui kuesioner tertutup kepada 16 responden pengelola proyek. Hasil penelitian menunjukan kualitas kontraktor ditinjau dari aspek sumber daya manusia, 59,4% menyatakan cukup baik, 50,0% menyatakan kurang baik pada asuransi tenaga kerja, dan 38,8% menyatakan kurang baik pada pemahaman tenaga teknik. Ditinjau dari aspek keuangan/finansial, 63,3% menyatakan cukup baik, 35,0% menyatakan tidak baik pada pengajuan uang muka. Diitinjau dari aspek metode pelaksanaan, 74.8% menyatakan baik, 46,3% menyatakan kurang baik pada metode kerja. Hasil regresi sederhana pengaruh variabel aspek sumber daya manusia, 70,3% dengan nilai sig 0,000<0,005 yang diisyaratkan, aspek keuangan/finansial 88,1% dengan nilai sig 0,000<0,005 yang diisyaratkan, aspek metode pelaksanaan 84,1% dengan nilai sig 0,000<0,005 yang diisyaratkan. Aspek keuangan mempunyai pengaruh yang dominan dengan nilai R2 88,1% dan nilai beta 0,891.
Confirming the Calibration of ALMA Using Planck Observations
Authors: Farren Gerrit S. , Partridge Bruce , Kneissl Rüdiger , Aiola Simone , Datta Rahul , Gralla Megan , Li Yaqiong 
Published: September 2021

Abstract
We test the accuracy of ALMA flux density calibration by comparing ALMA flux density measurements of extragalactic sources to measurements made by the Planck mission; Planck is absolutely calibrated to subpercent precision using the dipole signal induced by the satellite’s orbit around the solar system barycenter. Planck observations ended before ALMA began systematic observations, however, and many of the sources are variable, so we employ measurements by the Atacama Cosmology Telescope (ACT) to bridge the two epochs. We compare ACT observations at 93 and ∼145 GHz to Planck measurements at 100 and 143 GHz and to ALMA measurements made at 91.5 and 103.5 GHz in Band 3. For both comparisons, flux density measurements were corrected to account for the small differences in frequency using the best available spectral index for each source. We find the ALMA flux density scale (based on observations of Uranus) is consistent with Planck. All methods used to make the comparison are consistent with ALMA flux densities in Band 3 averaging 0.99 times those measured by Planck. One specific test gives ALMA/Planck = 0.996 ± 0.024. We also test the absolute calibration of both ACT at 93 and ∼145 GHz and the South Pole Telescope (SPT) at 97.43, 152.9, and 215.8 GHz, again with reference to Planck measurements at 100, 143, and 217 GHz, as well as the internal consistency of measurements of compact sources made by all three instruments.
L’épidémiologie pour comprendre le développement dans le TSA : l’exemple de la cohorte ELENA
Authors: Baghdadli Amaria 
Published: September 2021

L’épidémiologie est une discipline de la médecine vouée à l’étude des patterns liés à la santé et aux processus pathologiques dans les populations. Comme dans le reste de la médecine, les observations faites sur la fréquence des maladies ou leurs déterminants sont à la base de mesures de prévention et de traitement. Les applications plus récentes de l’épidémiologie en psychiatrie ont contribué chez l’enfant à une meilleure compréhension des troubles du neurodéveloppement. Ainsi, l’épidémiologie du développement consacre l’étude des processus développementaux et de leurs déterminants abordés au travers de multiples dimensions, biologiques, environnementales et psychologiques. Dans cet article, nous illustrons les apports de l’épidémiologie du développement dans la compréhension des facteurs de risque et de protection des trajectoires précoces de développement dans le trouble du spectre de l’autisme. Nous décrirons pour cela une étude de cohorte (ELENA) dans laquelle sont suivis prospectivement durant 6 années 900 enfants avec un diagnostic confirmé de TSA. L’objectif de cette étude est de décrire les trajectoires de développement de ces enfants et leurs déterminants aux plans biologiques, environnementaux et psychologiques.
Études longitudinales dans le TSA et position développementale
Authors: Pry René 
Published: September 2021

À partir d’une situation empruntée aux personnes sur le « Spectre », sont suggérées quelques contraintes que devrait respecter une recherche longitudinale. Apprécier un changement nécessite en effet d’en appréhender le contenu, d’en définir la fenêtre temporelle d’apparition, d’en dessiner les trajectoires évolutives et d’en évoquer les mécanismes. Mais il sera également suggéré de proposer une théorie du phénomène étudié qui intègre les changements, d’adopter un plan d’expérience adapté à ces derniers, d’utiliser des indicateurs pertinents et un appariement entre traitement statistique et modèle théorique.
ERIC, chronique d’une équipe mobile d’urgence et de crise
Authors: Zeltner Laure 
Published: September 2021

Depuis 27 ans ERIC (Equipe Rapide d’Intervention de Crise) déploie son activité dans les Yvelines sud et propose des alternatives à l’hospitalisation en psychiatrie. Comment le projet est-il né, s’est-il développé ? Quelles en sont ses missions ? Ses innovations ? La crise COVID est venue bousculer le principe de mobilité de l’équipe alors que sa disponibilité avait été réduite au 1er décembre 2020 par l’arrêt de la garde de nuit dans un contexte de pénurie médicale et infirmière territoriale. Comment alors poursuivre l’alternative à l’hospitalisation avec l’aide des proches quand la distanciation sociale est érigée comme règle et les protections comme denrées rares ?
La crise et l’urgence psychiatriques vécues par les familles
Authors: Viateau Jocelyne , Priolet Denys , Broutin Catherine , Monnier Alain , Pilet Marie-Françoise , Richard Marie-Jeanne 
Published: September 2021

L’urgence, telle qu’elle est perçue et vécue par ceux qui, un jour ou l’autre, se retrouvent dans la nécessité de recourir à ses services. Après avoir évoqué la situation générale et le contexte du recours aux urgences (déni du malade, représentation sociale de dangerosité du malade psychique, insuffisance du nombre de dispositifs d’aide en dehors de l’hôpital…), l’article, très constructif, évoque de nombreuses pistes d’amélioration possibles, techniques comme humaines, des situations d’urgences : prévention des situations de crise, gestion de la crise, accueil à l’hôpital, soutien aux aidants… susceptibles de diminuer les souffrances et les pertes de chances thérapeutiques pour les personnes malades et leurs familles.
A study to compare nasopharyngeal airway space in skeletal Class I, Class II, and Class III malocclusion
Authors: Tanpure Vijaysinh Ramchandra , Kalavani S. V. , Palagiri Firoz Babu , Bhagyalakshmi K. , Devaki Satish Babu 
Published: September 2021

Background:
Studies in the past have assessed relation between airway and type of malocclusion and found no association between rhinomanometric measures of airway adequacy and type of malocclusion or craniofacial morphology. Hence, the present study was undertaken to test whether there is any association between pharyngeal airway and type of malocclusion.

Methodology:
The subjects were divided into 3 groups based on skeletal pattern with 20 subjects in each group: Group 1 with Class I skeletal pattern, Group 2 with Class II skeletal pattern, and Group 3 with Class III skeletal pattern. All the cephalograms were taken in natural head position.

Results:
There is no significant difference found in total nasopharyngeal area between 3 groups. However, aerial area was significantly reduced in Group 3 (398.4 mm2) than that of Group 2 (485.737 mm2) (P = 0.012). Whereas, significant reduction in adenoidal area was observed in Group 2 (184.021 mm2) when compared with that of Group 3 (286.183 mm2).

Conclusion:
There was no statistically significant relationship between different skeletal groups and nasopharyngeal soft-tissue characteristics, except for two measurements i.e. aerial and adenoidal area of Group 2 and Group 3.
Performance comparison of commercial RNA extraction kits for the diagnosis of SARS-CoV-2
Authors: Perumal Nagaraj , Jain Rajeev Kumar , Sharma Archa , Chaurasia Deepti 
Published: September 2021

Introduction:
The COVID-19 pandemic has resulted in an increased need for molecular diagnostics. Ribonucleic acid (RNA) extraction is a crucial step in the detection of severe acute respiratory syndrome coronavirus-2 (SARS-COV-2). The resulting outcome depends on the performance of the RNA extraction kit used.

Methods:
This study evaluated the efficiency of four different commercial RNA extraction kits for the detection of SARS-COV 2. The reproducibility and performance efficiency of each kit were assessed and compared. The performance, processing time and storage conditions were also considered in the evaluation.

Results:
A slightly lesser cycle threshold (Ct) values were observed in RNA extracted using HiPurA Viral RNA Purification Kit. Invitrogen PureLink RNA Mini Kit was found to be time-saving and user-friendly.

Conclusions:
All the RNA isolation kit yielded the best results; however, if time is the important considerations, Invitrogen PureLink RNA Mini Kit has the shortest processing time.
A study on origin, termination, and course characteristics of internal thoracic artery relevant to coronary surgeries and reconstructive procedures
Authors: Agnihotri Gaurav , Mitra Anureet 
Published: September 2021

ABSTRACT

Objectives:
The internal thoracic artery is a favored vessel for coronary artery bypass grafting and is utilized for breast reconstructive surgeries. Our study focuses on the origin, termination, and course characteristics of the internal thoracic artery. A comprehension of these morphological features and possible variations will definitely aid a clinician in appropriate harvesting of the artery for clinical procedures.

Materials and Methods:
200 thoracic halves (from 100 embalmed adult human cadavers of either sex) were obtained from the department of anatomy. The origin, course characteristics, termination levels, and patterns for the internal thoracic artery were studied.

Results:
The internal thoracic artery originated from the first part of subclavian artery. The most common course pattern observed was medial concavity (88.5%). In 10% of cases, a tortuous course was observed. No artery with lateral concavity or rectilinear course pattern was documented. The artery terminated in the sixth space in 93.5% of cases. In 98% of cases, bifurcation in termination was observed. Trifurcation in termination was also observed in 2% of cases. The average length of variant artery (third terminating branch) was documented to be 5.5 cm.

Conclusion:
The increased utilization of the internal thoracic artery for coronary bypass arterial surgery and its role in sternal wound healing has made it imperative for clinicians to keep in mind its anatomical characteristics and local variations. This knowledge definitely will improve prognosis and decrease intraoperative/postoperative complications in patients undergoing coronary surgeries, percutaneous subclavian catheterizations, and reconstructive procedures.
Transarterial chemoembolization with or without sorafenib for hepatocellular carcinoma
Authors: Peng Tzu-Rong , Wu Ta-Wei , Wu Chao-Chuan , Chang Sou-Yi , Chan Cheng-Yi , Hsu Ching-Sheng 
Published: September 2021

Objectives:
Transarterial chemoembolization (TACE) or sorafenib may prolong survival in patients with unresectable hepatocellular carcinoma (HCC); however, whether their combination prolongs survival than TACE alone remains controversial. We aimed to compare the overall survival (OS) of patients with unresectable HCC treated with TACE plus sorafenib (TACE-S) versus TACE alone.

Materials and Methods:
All patients with unresectable HCC who received TACE as the initial therapy between January 2006 and January 2017 at Taipei Tzu Chi Hospital were enrolled. We matched patients treated with TACE-S and those treated with TACE alone (TACE) by performing propensity score matching at a 1:2 ratio. Our primary outcome was OS during a 10-year follow-up period, and represented as a hazard ratio calculated using Cox proportional hazard regression models.

Results:
Among 515 patients with unresectable HCC were treated initially with TACE, 56 receiving TACE-S group and 112 receiving TACE alone (TACE group) were included in the primary outcome analysis. The TACE-S group had significantly longer median OS than did the TACE group (1.55 vs. 0.32, years; P < 0.001), and the 5-year OS rates was 10.7% in the TACE-S group and 0.9% in the TACE group (P < 0.001). In multivariate analyses, patients with a lower Child–Pugh score, tumor size ≤5 cm, and no extrahepatic metastasis before treatment and those receiving antiviral agents and receiving TACE-S had longer OS (all P < 0.001).

Conclusion:
Antiviral agents and the combination of TACE with sorafenib may improve the OS of patients with unresectable HCC.
Quality of injectable oxytetracycline circulating in legal markets of Addis Ababa, Ethiopia using physiochemical and sterility analysis
Authors: Nigussie Yosef , Melaku Achenef , Tadese Misgana , Belete Bizuayehu , Kebede Elias 
Published: September 2021

This study was conducted to evaluate the quality of different brands of injectable oxytetracycline solutions circulating in the legal markets of Addis Ababa with respect to physicochemical characteristics and sterility. Nine brands of oxytetracycline with thirteen different batches were randomly purchased from veterinary drug stores in the city. The physical assessment was performed by using a checklist that was prepared based on the World Health Organization guidelines. The qualitative and quantitative analysis of active pharmaceutical ingredients (API) was performed by High-Performance Liquid Chromatography. The sterility test was assessed by using the direct inoculation method. All samples passed the identity, the assay, and the sterility tests. However, there were statistically significant differences (P<0.05) among brands in the quantity of API. The highest percentage of the API was recorded in brand G (112.12%± 1.86) while the lowest was seen in brand H (92.61%± 1.5). This study revealed that all brands passed both physicochemical and sterility tests except for the differences in the API level among brands. Strict regulation, monitoring, and wider-scale surveillance are required to assure sustainable control of substandard, unsterile, and falsified pharmaceutical products in the country.