bluuebunny/crossref_metadata_2025_split huggingface dataset:
Protein suppresses both bitterness and oleocanthal-elicited pungency of extra virgin olive oil
Authors: Peyrot des Gachons Catherine , O’Keefe Abigail J. , Slade Louise , Beauchamp Gary K. 
Published: June 2021

AbstractThe Mediterranean diet, considered one of the healthiest in the world, is characterized in part by the major source of its fat, which is extra virgin olive oil (EVOO). Among the health benefits of consuming EVOOs is the presence of phenolic compounds, which have been shown to lower the incidence of coronary heart disease and are suspected of providing many other health benefits. These phenolic compounds also contribute to the flavor of EVOO, adding both specific pungency in the throat and bitter notes that are valued by connoisseurs but reported to be unpleasant by naïve consumers. Here, we demonstrate that some food-derived proteins, specifically from egg yolks and whey, when added to pungent and bitter EVOOs, reduce or even eliminate both the throat pungency and bitterness. The sensory loss is proportional to the food protein additions. Thus, when used in various foods recipes (e.g. mayonnaise), pungent and bitter EVOOs may lose their pungent and bitter characteristics thereby rendering them more palatable to many consumers. This sensory reduction might also indicate interaction between the proteins and the phenolic compounds, which, if confirmed, would raise the question of whether the bioactivities of EVOO phenolics remain unchanged when consumed with and without protein-containing foods.
Investigation of the mechanisms of VEGF-mediated compensatory lung growth: the role of the VEGF heparin-binding domain
Authors: Yu Lumeng J. , Ko Victoria H. , Dao Duy T. , Secor Jordan D. , Pan Amy , Cho Bennet S. , Mitchell Paul D. , Kishikawa Hiroko , Bielenberg Diane R. , Puder Mark 
Published: June 2021

AbstractMorbidity and mortality for neonates with congenital diaphragmatic hernia-associated pulmonary hypoplasia remains high. These patients may be deficient in vascular endothelial growth factor (VEGF). Our lab previously established that exogenous VEGF164 accelerates compensatory lung growth (CLG) after left pneumonectomy in a murine model. We aimed to further investigate VEGF-mediated CLG by examining the role of the heparin-binding domain (HBD). Eight-week-old, male, C57BL/6J mice underwent left pneumonectomy, followed by post-operative and daily intraperitoneal injections of equimolar VEGF164 or VEGF120, which lacks the HBD. Isovolumetric saline was used as a control. VEGF164 significantly increased lung volume, total lung capacity, and alveolarization, while VEGF120 did not. Treadmill exercise tolerance testing (TETT) demonstrated improved functional outcomes post-pneumonectomy with VEGF164 treatment. In lung protein analysis, VEGF treatment modulated downstream angiogenic signaling. Activation of epithelial growth factor receptor and pulmonary cell proliferation was also upregulated. Human microvascular lung endothelial cells (HMVEC-L) treated with VEGF demonstrated decreased potency of VEGFR2 activation with VEGF121 treatment compared to VEGF165 treatment. Taken together, these data indicate that the VEGF HBD contributes to angiogenic and proliferative signaling, is required for accelerated compensatory lung growth, and improves functional outcomes in a murine CLG model.
The monoamine stabilizer OSU6162 has anxiolytic-like properties and reduces voluntary alcohol intake in a genetic rat model of depression
Authors: Melas Philippe A. , Wirf Malin , André Helder , Jayaram-Lindström Nitya , Mathé Aleksander A. , Steensland Pia 
Published: June 2021

AbstractAlcohol use disorders (AUD) often co-occur with anxiety and depressive disorders, and anxiety often drives relapse during alcohol abstinence. Optimal AUD pharmacotherapies may thus need to target both excessive alcohol intake and elevated anxiety. (−)-OSU6162 (OSU) is a monoamine stabilizer that attenuates alcohol-mediated behaviors in both preclinical and clinical settings. However, OSU’s effect on anxiety-like behavior following long-term drinking remains unknown. To this end, we utilized a genetic rat model that exhibits increased anxiety- and depression-like behaviors (Flinders Sensitive Line; FSL) and their controls (Flinders Resistant Line; FRL). Using the novelty suppressed feeding (NSF) test, we evaluated anxiety-like behaviors (1) at baseline, (2) following long-term voluntary drinking and after 24 h of alcohol deprivation, and (3) following OSU administration in the same animals. At baseline, FSL animals displayed significantly elevated anxiety-like characteristics compared to FRL. Compared to alcohol-naïve animals, long-term drinking significantly reduced anxiety-like behaviors in FSL, without any significant effects in FRL animals. Compared to vehicle, OSU administration significantly reduced anxiety-like behaviors in alcohol-naïve FSL and long-term drinking FRL animals. While there was no significant difference in alcohol intake between FSL and FRL, OSU attenuated alcohol intake in both strains. Conclusively, in addition to the compound’s previously identified ability to suppress alcohol-mediated behaviors, OSU may also possess anxiolytic properties, warranting further clinical evaluation in both AUD and anxiety disorder settings.
Stochastic Schrödinger equation derivation of non-Markovian two-time correlation functions
Authors: Carballeira Rafael , Dolgitzer David , Zhao Peng , Zeng Debing , Chen Yusui 
Published: June 2021

AbstractWe derive the evolution equations for two-time correlation functions of a generalized non-Markovian open quantum system based on a modified stochastic Schrödinger equation approach. We find that the two-time reduced propagator, an object that used to be characterized by two independent stochastic processes in the Hilbert space of the system, can be simplified and obtained by taking ensemble average over one single noise. This discovery can save the cost of computation, and accelerate the converging process when taking the average over noisy trajectories. As a result, our method can be widely applied to many open quantum models, especially large-scale systems and extend the quantum regression theory to the non-Markovian case. In the short-time simulations, it is observed a significant difference between Markovian and non-Markovian cases, which can be applied to realize the environmental spectrum detection and enhance the measurement sensitivity in varying open quantum systems.
Prognostic factors for mortality in invasive pneumococcal disease in adult: a system review and meta-analysis
Authors: Chen Hao , Matsumoto Hiromi , Horita Nobuyuki , Hara Yu , Kobayashi Nobuaki , Kaneko Takeshi 
Published: June 2021

AbstractRisk factors associated with mortality in invasive pneumococcal disease remain unclear. The present work is a meta-analysis of studies that enrolled only patients with invasive pneumococcal disease and reported on mortality. Potentially eligible reports were identified from PubMed, CHAHL, and Web of Science, comprising 26 reports in total. Overall mortality for invasive pneumococcal disease was reported as 20.8% (95% confidence interval (CI) 17.5–24%). Factors associated with mortality were age (odds ratio (OR) 3.04, 95% CI 2.5–3.68), nursing home (OR 1.62, 95% CI 1.13–2.32), nosocomial infection (OR 2.10, 95% CI 1.52–2.89), septic shock (OR 13.35, 95% CI 4.54–39.31), underlying chronic diseases (OR 2.34, 95% CI 1.78–3.09), solid organ tumor (OR 5.34, 95% CI 2.07–13.74), immunosuppressed status (OR 1.67, 95% CI 1.31–2.14), and alcohol abuse (OR 3.14, 95% CI 2.13–4.64). Mortality rates with invasive pneumococcal disease remained high, and these findings may help clinicians provide appropriate initial treatment for this disease.
Magnetic nanoparticles hyperthermia in a non-adiabatic and radiating process
Authors: Iglesias C. A. M. , de Araújo J. C. R. , Xavier J. , Anders R. L. , de Araújo J. M. , da Silva R. B. , Soares J. M. , Brito E. L. , Streck L. , Fonseca J. L. C. , Plá Cid C. C. , Gamino M. , Silva E. F. , Chesman C. , Correa M. A. , de Medeiros S. N. , Bohn F. 
Published: June 2021

AbstractWe investigate the magnetic nanoparticles hyperthermia in a non-adiabatic and radiating process through the calorimetric method. Specifically, we propose a theoretical approach to magnetic hyperthermia from a thermodynamic point of view. To test the robustness of the approach, we perform hyperthermia experiments and analyse the thermal behavior of magnetite and magnesium ferrite magnetic nanoparticles dispersed in water submitted to an alternating magnetic field. From our findings, besides estimating the specific loss power value from a non-adiabatic and radiating process, thus enhancing the accuracy in the determination of this quantity, we provide physical meaning to a parameter found in literature that still remained not fully understood, the effective thermal conductance, and bring to light how it can be obtained from experiment. In addition, we show our approach brings a correction to the estimated experimental results for specific loss power and effective thermal conductance, thus demonstrating the importance of the heat loss rate due to the thermal radiation in magnetic hyperthermia.
RUNX1 and REXO2 are associated with the heterogeneity and prognosis of IDH wild type lower grade glioma
Authors: Wang Haiwei , Wang Xinrui , Xu Liangpu , Zhang Ji , Cao Hua 
Published: June 2021

AbstractBased on isocitrate dehydrogenase (IDH) alterations, lower grade glioma (LGG) is divided into IDH mutant and wild type subgroups. However, the further classification of IDH wild type LGG was unclear. Here, IDH wild type LGG patients in The Cancer Genome Atlas and Chinese Glioma Genome Atlas were divided into two sub-clusters using non-negative matrix factorization. IDH wild type LGG patients in sub-cluster2 had prolonged overall survival and low frequency of CDKN2A alterations and low immune infiltrations. Differentially expressed genes in sub-cluster1 were positively correlated with RUNX1 transcription factor. Moreover, IDH wild type LGG patients with higher stromal score or immune score were positively correlated with RUNX1 transcription factor. RUNX1 and its target gene REXO2 were up-regulated in sub-cluster1 and associated with the worse prognosis of IDH wild type LGG. RUNX1 and REXO2 were associated with the higher immune infiltrations. Furthermore, RUNX1 and REXO2 were correlated with the worse prognosis of LGG or glioma. IDH wild type LGG in sub-cluster2 was hyper-methylated. REXO2 hyper-methylation was associated with the favorable prognosis of LGG or glioma. At last, we showed that, age, tumor grade and REXO2 expression were independent prognostic factors in IDH wild type LGG.
Choice of 16S ribosomal RNA primers affects the microbiome analysis in chicken ceca
Authors: Darwish Nadia , Shao Jonathan , Schreier Lori L. , Proszkowiec-Weglarz Monika 
Published: June 2021

AbstractWe evaluated the effect of applying different sets of 16S rRNA primers on bacterial composition, diversity, and predicted function in chicken ceca. Cecal contents from Ross 708 birds at 1, 3, and 5 weeks of age were collected for DNA isolation. Eight different primer pairs targeting different variable regions of the 16S rRNA gene were employed. DNA sequences were analyzed using open-source platform QIIME2 and the Greengenes database. PICRUSt2 was used to determine the predicted function of bacterial communities. Changes in bacterial relative abundance due to 16S primers were determined by GLMs. The average PCR amplicon size ranged from 315 bp (V3) to 769 bp (V4–V6). Alpha- and beta-diversity, taxonomic composition, and predicted functions were significantly affected by the primer choice. Beta diversity analysis based on Unweighted UniFrac distance matrix showed separation of microbiota with four different clusters of bacterial communities. Based on the alpha- and beta-diversity and taxonomic composition, variable regions V1–V3(1) and (2), and V3–V4 and V3–V5 were in most consensus. Our data strongly suggest that selection of particular sets of the 16S rRNA primers can impact microbiota analysis and interpretation of results in chicken as was shown previously for humans and other animal species.
Experimental observation of asymmetrical microwave jets and far-field distribution generated by a dual-material system
Authors: Varghese B. , Shramkova O. , Minard P. , Blondé L. , Drazic V. , Allié V. 
Published: June 2021

AbstractIn this paper, we report the experimental and numerical investigation of plane wave diffraction by an all-dielectric dual-material cuboid. Edge diffraction by a cuboid leads to the generation of a narrow, high intensity beam in the near-field region called a photonic jet. We examine the dependence of the jet behavior and orientation on the materials and dimensions of constitutive parts in the microwave frequency domain. The possibility to shift and deviate the resultant microwave jet in the near-field region of such a structure depending on the size of constitutive parts is demonstrated numerically. Experimentally, we observe a shift in the spatial position of the jet. The experimental asymmetric electric field profile observed in the far-field region is attributed to the input of multiple edge waves generated by the dual-material cuboid. The presented results may be scaled at different frequency bands such as optical frequencies for designing nanostructures enabling the focusing and deviation functionality and creation of new optical devices which would satisfy the needs of emerging nanophotonic applications.
Regulation of the acetylcholine/α7nAChR anti-inflammatory pathway in COVID-19 patients
Authors: Courties Alice , Boussier Jeremy , Hadjadj Jérôme , Yatim Nader , Barnabei Laura , Péré Hélène , Veyer David , Kernéis Solen , Carlier Nicolas , Pène Frédéric , Rieux-Laucat Frédéric , Charbit Bruno , Bondet Vincent , Duffy Darragh , Berenbaum Francis , Terrier Benjamin , Sellam Jérémie 
Published: June 2021

AbstractThe cholinergic system has been proposed as a potential regulator of COVID-19-induced hypercytokinemia. We investigated whole-blood expression of cholinergic system members and correlated it with COVID-19 severity. Patients with confirmed SARS-CoV-2 infection and healthy aged-matched controls were included in this non-interventional study. A whole blood sample was drawn between 9–11 days after symptoms onset, and peripheral leukocyte phenotyping, cytokines measurement, RNA expression and plasma viral load were determined. Additionally, whole-blood expression of native alpha-7 nicotinic subunit and its negative dominant duplicate (CHRFAM7A), choline acetyltransferase and acetylcholine esterase (AchE) were determined. Thirty-seven patients with COVID-19 (10 moderate, 11 severe and 16 with critical disease) and 14 controls were included. Expression of CHRFAM7A was significantly lower in critical COVID-19 patients compared to controls. COVID-19 patients not expressing CHRFAM7A had higher levels of CRP, more extended pulmonary lesions and displayed more pronounced lymphopenia. COVID-19 patients without CHRFAM7A expression also showed increased TNF pathway expression in whole blood. AchE was also expressed in 30 COVID-19 patients and in all controls. COVID-19-induced hypercytokinemia is associated with decreased expression of the pro-inflammatory dominant negative duplicate CHRFAM7A. Expression of this duplicate might be considered before targeting the cholinergic system in COVID-19 with nicotine.
Emergent synchronous beating behavior in spontaneous beating cardiomyocyte clusters
Authors: Sakamoto Kazufumi , Hondo Yoshitsune , Takahashi Naoki , Tanaka Yuhei , Sekine Rikuto , Shimoda Kenji , Watanabe Haruki , Yasuda Kenji 
Published: June 2021

AbstractWe investigated the dominant rule determining synchronization of beating intervals of cardiomyocytes after the clustering of mouse primary and human embryonic-stem-cell (hES)-derived cardiomyocytes. Cardiomyocyte clusters were formed in concave agarose cultivation chambers and their beating intervals were compared with those of dispersed isolated single cells. Distribution analysis revealed that the clusters’ synchronized interbeat intervals (IBIs) were longer than the majority of those of isolated single cells, which is against the conventional faster firing regulation or “overdrive suppression.” IBI distribution of the isolated individual cardiomyocytes acquired from the beating clusters also confirmed that the clusters’ IBI was longer than those of the majority of constituent cardiomyocytes. In the complementary experiment in which cell clusters were connected together and then separated again, two cardiomyocyte clusters having different IBIs were attached and synchronized to the longer IBIs than those of the two clusters’ original IBIs, and recovered to shorter IBIs after their separation. This is not only against overdrive suppression but also mathematical synchronization models, such as the Kuramoto model, in which synchronized beating becomes intermediate between the two clusters’ IBIs. These results suggest that emergent slower synchronous beating occurred in homogeneous cardiomyocyte clusters as a community effect of spontaneously beating cells.
Swimming droplets in 1D geometries: an active Bretherton problem
Authors: de Blois Charlotte , Bertin Vincent , Suda Saori , Ichikawa Masatoshi , Reyssat Mathilde , Dauchot Olivier 
Published: June 2021

Active droplets in capillaries swim at a speed set by physico-chemistry independent of their confinement, which is confirmed theoretically by an active Bretherton model. They spontaneously divide under strong confinement leaving behind dead droplets.
Cholesterol metabolism: a new molecular switch to control inflammation
Authors: Cardoso Diana , Perucha Esperanza 
Published: June 2021

Abstract
The immune system protects the body against harm by inducing inflammation. During the immune response, cells of the immune system get activated, divided and differentiated in order to eliminate the danger signal. This process relies on the metabolic reprogramming of both catabolic and anabolic pathways not only to produce energy in the form of ATP but also to generate metabolites that exert key functions in controlling the response. Equally important to mounting an appropriate effector response is the process of immune resolution, as uncontrolled inflammation is implicated in the pathogenesis of many human diseases, including allergy, chronic inflammation and cancer. In this review, we aim to introduce the reader to the field of cholesterol immunometabolism and discuss how both metabolites arising from the pathway and cholesterol homeostasis are able to impact innate and adaptive immune cells, staging cholesterol homeostasis at the centre of an adequate immune response. We also review evidence that demonstrates the clear impact that cholesterol metabolism has in both the induction and the resolution of the inflammatory response. Finally, we propose that emerging data in this field not only increase our understanding of immunometabolism but also provide new tools for monitoring and intervening in human diseases, where controlling and/or modifying inflammation is desirable.
Wafer‐fused 1300 nm VCSELs with an active region based on superlattice
Authors: Blokhin Sergey , Babichev Andrey , Gladyshev Andrey , Karachinsky Leonid , Novikov Innokenty , Blokhin Alexey , Rochas Stanislav , Denisov Dmitrii , Voropaev Kirill , Ionov Alexander , Ledentsov Nikolay , Egorov Anton 
Published: June 2021

AbstractThe 1300 nm range vertical‐cavity surface‐emitting lasers with the active region based on InGaAs/InGaAlAs superlattice are fabricated using molecular‐beam epitaxy and the double wafer‐fusion technique. Lasers with the buried tunnel junction diameter of 5 μm have shown single‐mode CW operation with the output optical power of ∼6 mW at 20°C. Opened eye diagrams are observed up to 10 Gbps.
Oxidative modifications and structural changes of human serum albumin in response to air dielectric barrier discharge plasma
Authors: Peng Siqi , Zhang Liyang , Zhang Dongheyu , Guo Yuntao , Wang Xinxin , Zhou Qun , Luo Haiyun 
Published: June 2021

AbstractIn this work, the air dielectric barrier discharge plasma was applied to a model of protein human serum albumin to explore plasma‐mediated protein damage from a biomolecular perspective. Carbonylation and side chain modifications of protein in solution were observed by Fourier transform infrared (FT‐IR) spectroscopy and mass spectrometry (MS). Protein backbone cleavage is proved by Bradford assay, sodium dodecyl sulphate–polyacrylamide gel electrophoresis, and MS. Typical fragments are in the range of 10–12 kDa. The decrease in α‐helix and increase in β‐sheet indicated by FT‐IR are major protein secondary structure changes. Accordingly, a whole map of protein oxidative damage and structural changes in response to the plasma was postulated.
Experimental analyses of two‐body wave energy converters with hydraulic power take‐off damping in regular and irregular waves
Authors: Wu Shuang , Liu Yanjun , Qin Jian 
Published: June 2021

AbstractA flume experimental study of a two‐body wave energy converter (WEC) with a hydraulic power take‐off (PTO) was conducted under the conditions of both regular and irregular waves. The experimental results were analysed using the control variable method, and the effects of the wave elements and external load on the relative heave motion response and capture width ratio of the device are discussed. For the regular waves, three modes were found when analysing the variation of the relative heave motion response with the incident wave height, and the initial moving line of the WEC model was obtained. For the irregular waves, the experiment displayed two motion states. One was a following motion with a similar value to the incident wave period, and the other was a delayed motion with a larger value than the wave period. Based on the calculation of the capture width ratio of the system both under the regular and irregular wave conditions, the maximum value occurred at a frequency ratio of and a hydraulic load of p = 0.20. The capture width ratio in the irregular waves was approximately 10%‐40% higher than that of the regular waves for the same wave parameters.