bluuebunny/crossref_metadata_2025_split huggingface dataset:
Biological Characteristics And Genomic Analysis of A Novel Bacteriophage BUCT609 Infecting Stenotrophomonas Maltophilia
Authors: Han Ke , Fan Huahao , Li Fei , Tong Yigang 
Published: August 2021

Abstract
Stenotrophomonas maltophilia is widely distributed in nature and has a high isolation rate in nosocomial infections. Due to its potential application and important role in clinical practice, the relevant studies of S.maltophilia have received much attention. S.maltophilia phage BUCT609 (GenBank: MW960043) was isolated from hospital sewage with S.maltophilia strain No. 3015 as a host. Morphologically, it can be inferred as Podoviridae phage from the result of transmission electron microscopy (TEM). The electron microscopy also shows that the phage has an isometric capsid ~50 nm in diameter. The one-step growth curve demonstrated that the incubation period of 10 min and the burst size is 382 pfu/cell when its optimal multiplicity of infection (MOI) is 0.01. Phage BUCT609 had a high survival rate at pH 3 to 10 and tolerant temperature from 4 ℃ to 55 ℃. Next-Generation Sequencing (NGS) results demonstrated that its complete genome is linear double-stranded DNA of 43145 bp in length, and the GC content is 58 %. It has very little resemblance to other phages. The BlastN analysis shows that the genome of phage BUCT609 shares 22 % homology with S.maltophilia phage Ponderosa (GenBank: MK903280.1), and it encodes 56 putative proteins, of which only 25 have annotated function. Phage BUCT609 with a relatively large burst size and excellent survival ability in a wide pH and temperature range, suggests BUCT609 is a potential alternative for multi-drug resistance S.maltophilia therapy.
A comparative analysis of Heat Shock Factor 1 in Cancer
Authors: Choudhury Shouhartha 
Published: August 2021

Abstract
The heat shock factor 1 (HSF1) is a DNA-binding factor that has responses under proteotoxic stress is well-known as an HSF. Heat shock factor 1 is a unique regulator that coordinates chaperone expression to enhance organism growth. Specifically, HSF’s drive transcriptional strategy is apparent from the HSP’s and promotes cell viability. A recent study supported the HSF1 coordinated with different cancer and robust in cancer immunotherapy. In this research, I designed to genome-wide analysis of HSF1 and its family in mammals. A comparative analysis of HSF’s is mandatory to explore functions and immunogenic mechanisms involved with HSP’s in organisms. Therefore, I performed a bioinformatics pipeline and tools to the current knowledge of HSF’s in two different organisms. This application can enhance in a significant way to study particular HSF’s in discrete organisms. My finding data demonstrated the number of HSF1 and their family in Homo sapiens and Mus musculus. The conserved domain, motifs, phylogeny, chromosome location, gene network, and expression analysis documented the HSF1 associated with cancer. The analysis data concluded the HSF1 is impact in various cancers and a signature of cancer immunotherapy.
A New Risk Score Assessment for Neck Management in T1-T2N0 Sinonasal Squamous Cell Carcinoma Patients: A European Head and Neck Cancer Center Survey Study and Evaluation of 58 Patients with Early Stage Sinonasal Carcinoma
Authors: Lill Claudia , Erovic Boban , Seemann Rudolf , Faisal Muhammad , Stelter Klaus , Gandler Bernd , Frommlet Florian , Janik Stefan 
Published: August 2021

Abstract
Purpose

The purpose of the present study was to assess whether European head and neck cancer centers perform elective neck dissection (END) in early stage sinonasal squamous cell carcinomas (SCCs) and whether END affects oncological outcome.
Methods

A questionnaire regarding performance of END in T1 - T2 sinonasal SCCs was sent to 38 head and neck cancer centers in Europe. The results were further correlated and compared with clinical data of 58 patients with T1 (n = 37) and T2 (n = 21) sinonasal SCCs, a risk score and a nomogram were generated.
Results

Only 5–10 % of respondents are performing END in T1 and 26–32% in T2 sinonasal SCCs. In our cohort regional recurrence was evident in 10 (17.2%) patients, representing a significant worse prognostic factor for cancer specific survival (HR 8.13; p = 0.016). Particularly, regional recurrence was more frequent in T2 tumors and in patients where the primary tumor originated from nasal septum and vestibule. Based on our clinical and questionnaire data a new risk-score was determined to predict necessity for END. The risk-score, including T-classification and tumor site, assigned T1-classification with 0 points, followed by 1 point for T2; tumor site: nasal septum or vestibule with 1 point and tumors from other subsites with 0 points. Based on these factors we further created a nomogram for predicting the risk of regional recurrence. Patients with a high-risk score showed 9.52-fold higher risk for regional and or distant recurrence (HR 9.52; p = 0.002;) and their 5-year CSS was 44.4% compared to 92.8% in patients with moderate or low risk (p = 0.017).
Conclusions

Our proposed risk-score system in T1-2N0 sinonasal carcinoma is helpful to identify patients who may benefit from END. Although to date END has no value in European head and neck cancer centers for T1-T2 sinonasal SCCs, our study indicates that the neck management in these patients should be reconsidered.
Covid-19 in Liver Transplant Recipients: An Italian Case-Control Double-Centre Study.
Authors: Guarino Maria , Cossiga Valentina , Loperto Ilaria , Esposito Ilaria , Ortolani Rosanna , Fiorentino Andrea , Pontillo Giuseppina , De Coppi Lucia , Cozza Valentina , Lanza Alfonso Galeota , Costanzo Giovanni Giuseppe Di , Picciotto Francesco Paolo , Morisco Filomena 
Published: August 2021

Abstract
Background and Aims: Liver transplant (LT) recipients are vulnerable to SARS-CoV-2-infection (COVID-19), due to immunosuppression and comorbidities. This study aimed to evaluate the impact of COVID-19 on LT recipients compared to general population in the Campania region.Methods: In this case-control double-centre study, we enrolled all consecutive adult LT recipients with confirmed SARS-CoV-2-infection. Data were collected at diagnosis of COVID-19 and during follow-up and compared with the regional population. Results: Thirty LT recipients (3.28%) developed SARS-CoV-2-infection (76.6% male, median age 62.6 years). Sixteen (53.3%) were symptomatic. Common symptoms were fever, cough, fatigue, and anosmia. Twenty-five (83.4%) were outpatients, 5 (16.6%) required hospitalization (6.6% admitted to Intensive Care Unit, 6.6% developed Acute Respiratory Distress Syndrome and 6.6% died). Immunosuppressors were in 3 (10%) patients. Incidence rate of COVID-19 was similar between LT patients and general population (3.28% vs 4.37%, p=0.1) with higher rate of symptoms in LT patients (46.67% vs 15.87%, p<0.00). At univariate analysis, hospitalization and case fatality rates were higher in LT patients compared to general population (16.67% vs 4.54%, p=0.001; and 6.67% vs 1.76%, p=0.04, respectively). At multivariable logistic regression analysis, LT patients with COVID-19 were more frequently symptomatic (OR 5.44 [95%CI 2.44-12.17], p<0.00), whereas hospitalization and death for COVID-19 were not significatively associated with LT condition (p=0.72 and p=0.46, respectively) and were comparable with general population.Conclusions: LT is not a risk factor for acquiring COVID-19. Nonetheless, LT patients are more frequently symptomatic, although comparable to the general population for hospitalization rate and mortality.
Silicon Wearable Body Area Antenna for Speech Enhanced IOT and Medical Applications
Authors: Vignesh N Arun , S Kanithan , S Jana , C Gokul Prasad , E Konguvel , Panigrahy Asisa Kumar 
Published: August 2021

Abstract
We propose in this paper a reduction in the size of wearable antennas on silicon (Si) for medicinal frameworks and Internet of things (IoT). This research also introduces one more type of dynamic patch antenna designed in favor of speech-enhanced healthcare applications. The most significant impediment to the adoption of smart correspondence and medical services frameworks is voice-enabled IoT. The primary objective of a body area network (BAN) is to give ceaselessly clinical information to the doctors. Actually wireless body area network is flexible, dense, trivial and less expensive. On the other hand the main disadvantage is low efficiency for small printed antenna. Microstrip silicon antenna recurrence is changed because of ecological conditions, distinctive reception apparatus areas and diverse framework activity modes. By using tunable antenna the efficiency of bandwidth usage can be increased. Amplifiers are associated with the feed line of antenna in order to build its dynamic range. In this study, a dynamic polarized antenna is constructed, analysed and attempted for fabrication. The gain of the antenna is 13 ± 2dB for the frequency range of 390 to 610MHz. The output of the polarized antenna is roughly 19 dBm. At different environmental conditions the performance and ability to control the antenna could vary. To achieve stable performance, we have used varactor diode and voltage controlled diode. This silicon wearable antenna can be fabricated and tested for many medical applications like health monitoring system, pacemakers etc. Furthermore, Micromachining techniques can be used to lower the practical dielectric constant of Silicon and hence improve radiation efficiency.
Weight Change, Lifestyle and Mortality in Patients With Type 2 Diabetes
Authors: Hu Jinbo , Hu Yang , Hertzmark Ellen , Yuan Chen , Liu Gang , Stampfer Meir J. , Rimm Eric B. , Hu Frank B. , Wang Molin , Sun Qi 
Published: August 2021

Abstract
Background: Whether weight change around type 2 diabetes (T2D) diagnosis is associated with long-term survival is unclear. We aimed to examine the association between weight change and mortality among participants with incident T2D and evaluate impacts of lifestyle on this association. Methods: This prospective analysis included 11,262 incident T2D patients from the Nurses’ Health Study and Health Professionals Follow-up Study. We assessed weight change bracketing T2D diagnosis in relation to mortality. We also examined potential effect modification by a healthy lifestyle consisting of high-quality diet, regular physical activity, non-smoking status and moderate alcohol consumption.Results: On average, T2D patients lost 2.3 kg during a two-year time-window spanning the T2D diagnosis, and body weight increased afterwards. Compared with patients with a stable weight, T2D patients who lost ≥10% body weight had a 26% (95% CI: 4%, 52%) increased mortality due to cardiovascular disease (CVD). Lifestyle significantly modified these associations: the hazard ratios (95% CIs) of CVD mortality comparing ≥10% weight loss with stable weight were 1.41 (0.87, 2.30) among participants with a deteriorated lifestyle, 1.46 (1.15, 1.86) for a stable lifestyle, and 0.82 (0.53, 1.27) for an improved lifestyle (Pinteraction <0.001). Major weight loss was also associated with an increased all-cause mortality, and similar effect modifications by lifestyle were observed.Conclusions: Significant weight loss upon T2D incidence was associated with an increased CVD mortality, although improved lifestyle quality abolished these associations. These results highlight the role of adopting a healthy lifestyle for newly diagnosed T2D patients in improving long-term survival.
Primary Treatment of Small-To-Medium (&lt;3cm) Sporadic Vestibular Schwannomas: A Systematic Review And Meta-Analysis on Long-Term Hearing Preservation And Tumor Control Rates For Microsurgery Versus Radiosurgery.
Authors: Savardekar Amey Rajan , Terrell Danielle L , Lele Saudamini Jayant , Diaz Ryan Robert , Keesari Praneeth Reddy , Trosclair Krystle , Kosty Jennifer , Wang Chiachien Jake , Gardner Gale , Guthikonda Bharat 
Published: August 2021

Abstract
BackgroundOver the past two decades, the treatment of small-to-medium (<3cm) sporadic vestibular schwannomas (VS) has experienced a definite shift to stereotactic radiosurgery vis-a-vis microsurgery. We performed a systematic review and meta-analysis of VS patients primarily treated with stereotactic radiosurgery (SRS) or microsurgery (MS), with particular attention to hearing preservation outcomes (HPO), tumor control (TC), and facial nerve dysfunction (FND).MethodsA systematic review was conducted (Medline and Scopus database) for the period, January 2010 to June 2020 with appropriate MeSH. English language articles for small-to-medium sporadic VS (<3cm) utilizing SRS or MS as treatment modality, with minimum follow-up of 3 years, were included. Studies had to report an acceptable standardized hearing metric. HPO, TC, and FND rates were analyzed. ResultsThirty-two studies met inclusion criteria: 10 for microsurgery; 23 for radiosurgery (one comparative study included in both). HPO, at ~65 months follow-up, were comparable between MS group (10 studies; 809 patients) and SRS group (23 studies; 1234 patients) (56% versus 59%, p=0.1527). TC, at ~70 months follow-up, was significantly better in MS group (9 studies; 1635 patients) versus SRS group (19 studies; 2260 patients) (98% versus 92%, p < 0.0001). FND, at ~ 12 months follow-up, was significantly higher in MS group (8 studies; 1101 patients) versus SRS group (17 studies; 2285 patients) (10% versus 2%, p < 0.0001).ConclusionMS and SRS are comparable primary treatment options for small (<3cm) sporadic VS with respect to HPO at 5-year follow-up in patients with serviceable hearing at presentation. Approximately 50% patients for both modalities will likely lose serviceable hearing by that time-point. High TC rates (>90%) were seen with both modalities, with MS (98%) significantly better than SRS (92%). The post-treatment FND was significantly less with SRS group (2%) versus MS group (10%).
The Portuguese Man-of-war, One of The Most Dangerous Marine Species, Has Always Entered The Mediterranean Sea: Strandings, Sightings And Museum Collections
Authors: Badalamenti Rosario , Tiralongo Francesco , Arizza Vincenzo , Brutto Sabrina Lo 
Published: August 2021

Abstract
A search from different kinds of sources has been carried out to review the incidence of Physalia physalis, the Portuguese man-of-war, in the Mediterranean Sea; scientific and grey literature, social media, zoological museums were accessed. The records of the species were considered validated if documented with images or collected specimens. It was possible to date the putative first record of Physalia physalis in the Mediterranean Sea, thanks to a couple of colonies preserved in a historical collection, originating from the Gulf of Naples in 1914. Some massive strandings occurred in localities of the Alboran Sea, area of entrance from the Atlantic from where the species spread mainly along the Sicilian waters, in the central Mediterranean Sea. The records from the Italian maritime regions were then subdivided into three categories of risk according to the season of occurrence. These categories were created to assign a level of danger for swimmers to the sightings of Physalia physalis. The increasing sightings of such a poisonous organism in coastal waters can represent a risk to human health, and also to all those activities linked to the marine tourism sector. The involvement of citizens and touristic structures for the early detection of Physalia physalis can play a key role in preventing encounters with the species, allowing marine tourist facilities to operate within a range of reasonable security.
Application of cytokine-induced myeloid-derived suppressor cells mitigates murine pancreatic insulitis through inhibition of activated T cells
Authors: hsieh ching chuan , Lin Chun-Liang , Chiang Meihua , Chang Yong-Lin , He Jie-Teng , Hung Chien-Hui 
Published: August 2021

Abstract
Background

Type 1 diabetes is an inflammatory state. Myeloid-derived suppressive cells (MDSCs) originate from immature myeloid cells and quickly expand to control host immunity during infection, inflammation, trauma, and cancer. Immunotherapy is a method of disease treatment that involves stimulating or suppressing an immune response. Although the spleens and blood of tumor-bearing mice are the most common MDSC source for immunotherapy, many safety precautions must be considered.
Methods

Cytokine-induced MDSCs were propagated from bone marrow cells cultured with mouse recombinant granulocyte-macrophage colony-stimulating factor (GM-CSF), interleukin (IL)-6, and IL-1β. Arginase-1 and inducible NO synthase (iNOS) expressions of MDSCs were evaluated using quantitative reverse-transcriptase PCR. Diabetes was diagnosed as a sustained blood sugar level > 350 mg/dL. The immunized T cells harvested from the spleens of nonobese diabetic (NOD) mice cotransplanted with cMDSCs were injected intravenously to the NOD mice with severe combined immune deficiency (SCID), twice a week for 5 consecutive weeks. Diabetes-free survival of NOD–SCID mice was measured. Fibronectin expressions of the renal glomerulus and insulin production of the pancreas were analyzed using immunohistochemistry studies.
Results

This study presents an ex vivo procedure to develop MDSCs from bone marrow cells propagated from GM-CSF, IL-6, and IL-1β cytokines expressing less costimulatory and more inhibitory surface markers as well as considerably high levels of the iNOS enzyme through the signal transducer and activator of transcription 1 (STAT1) signaling pathway. In addition, the MDSCs also displayed immature morphology and strong immunosuppression of T cell proliferation. The adoptive transfer of cMDSCs improved the hyperglycemic state in and prolonged the diabetes-free survival of NOD-SCID mice induced by reactive splenic T cells harvested from NOD mice. In addition, cMDSC use reduced fibronectin production in the renal glomeruli and improved pancreatic insulitis in the NOD–SCID mice.
Conclusions

The administration of cMDSCs propagated from GM-CSF, IL-6, and IL-1β cytokines provides an alternative immunotherapy protocol for the treatment of diabetic pancreatic insulitis and renal nephropathy.
Methylmercury Induced Apoptosis of Human Neuroblastoma Cells Through the ROS Mediated Caspase and PARP/AIF Dependent Pathways
Authors: Hou Shanshan , Zhang Xiayu , Ning Xiaofan , Wu Hao , Li Xinyue , Ma Kai , Hao Huifang , Lv Chunping , Li Chunrui , Du Zhongjun , Du Haiying , Jin Minghua 
Published: August 2021

Abstract
Methylmercury (MeHg) is an environmental neurotoxic substance, which can be absorbed by the human body through the digestive tract, and easily cross the blood-brain barrier, causing irreversible damage to the human central nervous system. Reactive oxygen species (ROS) are involved in various ways of intracellular physiological or pathological processes including neuronal apoptosis. The current studies attempted to explore the role of ROS-mediated PARP/AIF apoptosis signal in the process of MeHg inducing human neuroblastoma cells (SH-SY5Y) death. Here, the present studies found that SH-SY5Y cells underwent apoptosis in response to MeHg, which was accompanied by increased the levels of ROS and calcium ion, and the activation of caspase cascades and poly ADP-ribose polymerase (PARP). The decrease in ROS levels significantly reduced the expression of these proteins and the rate of apoptosis. Inhibition of caspase pathway can reduce the rate of apoptosis, but can not prevent the occurrence of apoptosis. Furthermore, inhibition of PARP signaling can significantly reduce the apoptosis rate and the expression of caspase pathway related proteins. Collectively, these results indicated that ROS mediated activation of caspase pathway and PARP /AIF signaling pathway are involved in MeHg induced apoptosis, and there is a certain relationship between the two pathways.
Significs of Lady Victoria Welby: Portrait in the interior of time
Authors: Скрипник К.Д. 
Published: August 2021

В статье рассматриваются идеи сигнифики – науки о значении, сама концепция которой возникает на рубеже XIX–XX вв. благодаря леди Виктории Уэлби (1837–1912), имя которой на протяжении длительного времени было незаслуженно забыто. Описываются основные факты ее жизни, показана связь характеристик личности Уэлби и идей сигнифики в контексте интеллектуальной атмосферы ее времени. Личность и работы леди Уэлби оказали влияние на развитие философских и семиотических исследований, а также на установление связей между мыслителями различных стран. Заслуга Уэлби – в создании целостной динамической теории знака и значения, теории, интегрирующей различные аспекты изучения значения – от логико-лингвистических до аксиологических и практических.
The publication considers the ideas of significs-the science of meaning, the ideas and the concept of which arise at the turn of the nineteenth and twentieth centuries thanks to their author –Victoria Lady Welby (1837–1912), whose name for a long time was undeservedly forgotten. It describes the main facts of her life, shows the relationship between the characteristics of Welby's personality and the ideas of significs in the context of the intellectual atmosphere of her time. The personality and work of Lady Welby influenced the development of philosophical and semiotic research, as well as the establishment of connections between scholars from different countries. Welby's contribution is to create an integral dynamic theory of sign and meaning, a theory that integrates various aspects of the study of meaning - from logical-linguistic to axiological and practical.
The fate of an official: publishing activities of A. I. Miretsky
Authors: Крылова Е.Н. 
Published: August 2021

В статье рассматривается судьба чиновника министерства финансов А.И. Мирецкого. На страницах организованного им в Киеве в 1911 г. журнала А.И. Мирецкий отражал настроения многочисленной группы малообеспеченных чиновников, недовольных условиями своего существования. Его деятельность способствовала знакомству чиновников с условиями труда и быта в других государственных учреждениях и частях Российской империи, осознанию ими собственных интересов и формированию стратегии по улучшению своего положения.
The article is devoted to the fate of an official of the Ministry of Finance, A.I. Miretsky, who established a magazine in Kiev in 1911. On the pages of his publication, Miretsky reflected the moods of a large group of low-income officials who were dissatisfied with the conditions of their existence. The publishing activity of Miretsky contributed to familiarizing officials with working and living conditions in other state institutions and parts of the Russian Empire, forming a strategy to improve their situation.