bluuebunny/crossref_metadata_2025_split huggingface dataset:
Alleviating heat stress during early-season establishment of containerized strawberry transplants
Authors: Dash Prosanta K. , Chase Carlene A. , Agehara Shinsuke , Zotarelli Lincoln 
Published: December 2021

BACKGROUND: Earlier planting under subtropical climatic conditions has been proposed as a means of increasing strawberry ( Fragaria× ananassa Duch.) profitability but exposes transplants to heat stress. OBJECTIVE: Therefore, physical and chemical methods of ameliorating heat stress on the establishment, growth, and yield of containerized strawberry transplants were evaluated. METHODS: We used a split-plot design with white-on-black (white) mulch and black mulch as main plot treatments; and a factorial arrangement of transplant type and stress-prevention application was randomly assigned to the subplots. ‘Florida Radiance’ Jiffy plug and tray plug transplants were treated either with s-abscisic acid or with kaolin. RESULTS: White mulch resulted in greater plant vigor and vegetative growth than black mulch. Kaolin plus white mulch provided protection against heat stress as indicated by increased photosynthesis rates. At 4 weeks after transplanting, plant vigor and shoot growth were higher and flowering occurred earlier with Jiffy plug transplants than with tray plug transplants. Early marketable yields were higher with white mulch than with black mulch in three of the four site years. CONCLUSIONS: A combination of white mulch, Jiffy plug transplants, and kaolin application appears to have the best potential for alleviating heat stress during early-season strawberry establishment under subtropical conditions.
BRCA1-Associated Protein 1 (BAP-1) as a Prognostic and Predictive Biomarker in Clear Cell Renal Cell Carcinoma: A Systematic Review
Authors: Gulati Shuchi , Previtera Melissa , Lara Primo N. 
Published: December 2021

BACKGROUND: The gene that encodes BRCA1-associated protein 1 (BAP1) has been reported to be dysregulated in several human cancers such as uveal melanoma, malignant pleural mesothelioma, hepatocellular carcinoma, thymic epithelial tumors, and clear-cell renal cell carcinoma (ccRCC). The gene is located on the human chromosome 3p21.3, encoding a deubiquitinase and acts as a classic two-hit tumor suppressor gene. BAP1 predominantly resides in the nucleus, where it interacts with several chromatin-associated factors, as well as regulates calcium signaling in the cytoplasm. As newer therapies continue to evolve for the management of RCC, it is important to understand the role of BAP1 mutation as a prognostic and predictive biomarker. OBJECTIVE: We aimed to systematically evaluate the role of BAP1 mutations in patients with RCC in terms of its impact on prognosis and its role as a predictive biomarker. METHODS: Following PRISMA guidelines, we performed a systematic literature search using PubMed and Embase through March 2021. Titles and abstracts were screened to identify articles for full-text and then a descriptive review was performed. RESULTS: A total of 490 articles were initially identified. Ultimately 71 articles that met our inclusion criteria published between 2012–2021 were included in the analysis. Data were extracted and organized to reflect the role of BAP1 alterations as a marker of prognosis as well as a marker of response to treatments, such as mTOR inhibitors, VEGF tyrosine kinase inhibitors, and immune checkpoint inhibitors. CONCLUSIONS: Alterations in BAP1 appear to be uniformly associated with poor prognosis in patients with RCC. Knowledge gaps remain with regard to the predictive relevance of BAP1 alterations, especially in the context of immunotherapy. Prospective studies are required to more precisely ascertain the predictive value of BAP1 alterations in RCC.
DETERMINATION OF COLORED SURFACE PROPERTIES ART OBJECT AS A MEANS OF CREATING A HARMONIOUS ARCHITECTURAL ENVIRONMENT
Authors: Pylypchuk Oksana , Polubok Andrii , Krivenko Olha 
Published: December 2021

The article examines the properties of the colored surface of the Art object as a means of creating a harmonious architectural environment. The question was raised about the relevance of increasing the comfort, functionality, ergonomics, environmental friendliness and aesthetics of the design of the architectural environment, which is associated with global problems caused by the deterioration of the environment and unprecedented measures in connection with the pandemic. Taking into account the fact that the introduction of elements of fine art into the architectural space is always accompanied by only positive emotions, the necessary design of various types of Art objects in the modern architectural space has been determined. It is indicated that the artistic material is the main factor in the objects of fine art, capable of participating in the formation of the aesthetic perception of the environment. The analysis of the existing sources of theoretical and practical experience has shown the relevance of this problem.
Based on the results of the analysis, the main properties of the colored surface were systematized, identified and determined, which affect the perception of the texture of colored surfaces of different types of art forms of Art objects, depending on the following factors: 1) color saturation and lighting; 2) distance from observer; 3) property of the surface of the material and the nature of its processing; 4) the color tone of the material surface, its saturation and lightness. Methods of its use in different types of Art objects are proposed. Typical examples of the practical implementation of the use of Art objects using the possibilities of a colored surface in a modern architectural space, made by the authors of the study and modern examples of world art, are presented in a visual table.
Based on the results of the study, the main tools were identified that may be necessary in the design practice of architects, designers, artists to create a harmonious architectural environment.
SPATIAL PLANNING OF TERRITORIES AS A NEW TYPE OF URBAN PLANNING ACTIVITY. PROBLEMS AND PROSPECTS
Authors: Stepanyuk Andriy , Kiuntsli Romana 
Published: December 2021

Administrative and territorial reform in Ukraine is coming to an end. In the socio-economic life of the country, this reform contributed to the establishment of local self-government, in urban planning activities began spatial planning, the main task of which is the development of comprehensive plans for spatial development of the territories of united territorial communities.
According to the current legislation, the main subject of local self-government in Ukraine is a united territorial community. Adoption of the Law 711-IX "On Amendments to Certain Legislative Acts of Ukraine on Land Use Planning" provides for the development of special urban planning documentation, which extends its effect to the community, which in turn allows the united territorial community to determine the planning and development of its own territory. Law 711-IX also introduces a new type of urban planning documentation - a comprehensive plan for the spatial development of the territory of the united territorial community. In order to successfully implement this project, it is necessary to assess all the challenges and risks in the field of administrative services, medicine, education, road and engineering infrastructure, environmental protection and cultural heritage when drawing up a community spatial development plan in modern conditions.
The main problem in drawing up comprehensive plans is the spatial organization of agricultural areas and the interests of the peasant farmer, a representative of the middle class, whose opinion should be taken into account through surveys and analysis of his social and industrial activities, including taking into account his domestic and economic interests.
When drawing up comprehensive spatial development plans, the project team should involve community specialists (architect, land surveyor), as well as community proxies (priests, teachers) who know regional issues, history and traditional crafts of the territories and will defend their development. As many community residents as possible should participate in the discussion and approval of project proposals for a comprehensive plan, in order to prevent resistance and understand the need to implement it.
Recent Developments of Recombinant Protein Expression for Therapeutic Purposes
Authors: Amir Anam 
Published: December 2021

In the most recent seven to eight years, the therapeutic recombinant proteins have rapidly expanded in the biotechnology domain due to its wide variety of needs. There has been significant development in the mammalian expression system for fine purification and increased level of expressed recombinant proteins [1,2]. Many drugs like tetracycline have been demonstrated on the Chinese Hamster Ovary cell line for promising multi control strategies and effective cytotoxicity. Mammalian expression system improves the proper glycosylation of recombinant proteins which are very helpful to increase solubility of product [3-6].
Meanwhile on the prokaryotic expression system, E. coli has proven to be an easier to handle, friendly and economical strain [2]. Recently these expression systems are using to work on antibody fragment productions and their proper folding with co-expression of chaperones [7]. Moreover E. coli has been used for the production of cancer cell penetrating peptides which promises the targeted delivery of drugs to specific effector cells only. Yeast systems are also being used for the antibody fragments production and the high level production of insulin. Interestingly cell free expression systems are also participating in this game and that would be very fascinating to see in the coming years about cell extract medium for production of high level recombinant protein [8, 9]. Purification and optimization of recombinant protein has always been a challenging situation for scientists and they paid more attention to increase the overall yield of the product. Many affinity chromatography techniques has been introduced for efficient purification of protein of interest [10].
Despite these research and developments in methodologies to produce and purify the recombinant therapeutic protein, scientists still face the hurdles and challenges with all expression systems. Rationally E. coli produces inclusion bodies and many mammalian cell types do not show the same results with the same recombinant protein. [11]. So there is a requirement for adding the appropriate features to the expression systems focused to better improvising recovery, production and purification of recombinant protein.
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The Presence of Helicobacter pylori in cases of Chronic Tonsillitis in Medan
Authors: Harahap Rini Syahrani , Delyuzar  , Jessy Chrestella  
Published: December 2021

Background: Helicobacter pylori is a well known pathogen which is recognized as the most frequent infection of individuals. Helicobacter pylori plays important role in the etiology og gastritis, gastric and duodenal ulcers. We investigated the presence of Helicobacter pylori as an extragastric reservoir in the tonsilectomy specimens.
Aim: To determine the presence of Helicobacter pylori in cases of chronic tonsillitis in tonsilectomy specimens.
Methods: This is a descriptive study with cross sectional which the sample will be obtained using consecutive sampling method. We collect 43 cases with chronic tonsilitis were examined in tonsillectomy specimens for which available tissue for routin staining could be retrieved were histopathology chronic tonsillitis. The colonization of the helicobacter pylori have been evaluated with hematoxylin & Eosin and Giemsa stains under the light microscope.
Results: With H&E staining, Helicobacter pylori has been detected in 23 cases (53,48%) of the tonsil-lectomy specimens in total. No colonization has been observed in the remaining 20 cases (46,51%). With giemsa staining Helicobacter pylori has been detected in 29 cases (67,74%) of the tonsillectomy specimens in total. No colonization has been observed in the remaining 14 cases (32,55%).
Keywords: Helicobacter pylori, Chronic Tonsillitis, H&E, Giemsa.
Description Of Bag Load With Antropometry Of Elementary School Students
Authors: Krishna Moorthi Priya Darshini , Machrina Yetty , Laksmi Lidya Imelda , Yaznil Muhammad Rizki 
Published: December 2021

Background. Low back pain is one of the most common health problem among school students. Carrying heavy backpack to school is one of the cause of low back pain. Objectives. To identify the bag load carried by the students and antropometry of the students. Method. This research is a descriptive observasional with cross sectional method. Data used is a primer data which is from 268 students by measuring their body weight, height, bag weight and also by interviewing them using interview guidelines. Results. The results obtained from 268 elememtary school students of Siti Hajar mostly have normal nutritional status. The average bag weight at level I (6.071%), level II (8.510%) and level III (10.812%) where the majority of bag loads compared to elementary school antropometry were included in the mild category 47.6% at level I, 74.7% at level II and 84.3% at level III eventhough there were some children in the heavy category. 88.8% of children carry their school bags using two shoulders, with a duration of time <30 minutes (61.7%). Respondents who experienced back pain (4.9%) and who did not experience back pain (95.1%). Conclusions. The weight of Siti Hajar Elementary School children bag against antropometry has not exceeded the recommended load limit.
Key words. Bag load, antropometry, cross-sectional, interview
The Effect of Work from Home and Work Load on Work-Life Balance of Generation X and Generation Y Employees
Authors: Pasla Peter , Asepta Uki , Widyaningrum Santi , Pramesti Monike , Wicaksono Soetam 
Published: December 2021

Work-Life Balance (WLB) is a situation where a person manages to balance work, family life and other responsibilities. During the COVID-19 pandemic, which began by the end of 2019, many companies have implemented a Work from Home (WFH) policy that allows a worker to spend all his time at home and with a relatively light workload. The workforce today onward will be dominated by employees from generation X, namely people aged between 45 to 56 years and people from generation Y, who are 27-44 years of age. Each generation has its own characteristics of how they work and respond to work situations. This research was conducted in an effort to determine the impact of WFH and workload on Generation X and Generation Y. The population in this study were generation X and Y in Malang City, who had carried out WFH. Using the Slovin formula, the samples were 228 male and 178 female workers of Generation X and Generation Y. The results of this study indicated that WFH did not significantly affect the WLB of Generation X and Generation Y while workload had a significant effect on the WLB of Generation X and Generation Y.
Indicators of mineral bone density in male patients with osteoarthritis of knee joint of elderly and old age depending on condition of muscular system
Authors: Koroleva M. V. , Letaeva M. V. , Raskina T. A. , Malyshenko O. S. , Averkieva Y. V. 
Published: December 2021

Objective. To evaluate bone mineral density (BMD) indices in elderly and old age male patients with knee osteoarthritis (OA) depending on the state of the muscular system.Subjects and methods. The trial enrolled 32 patients with knee OA. The mean age of the patients was 68.7 (66.1; 70.3) years. The diagnosis of knee OA was established according to the diagnostic criteria of the American College of Rheumatologists (ACR, 1991). For comparative analysis, all patients with knee OA were divided into 3 groups according to muscle condition (EWGSOP, 2010: muscle mass was assessed by multispiral computed tomography, muscle strength was measured using a wrist dynamometer, muscle function was examined using a battery of short tests): the 1st – 9 patients without sarcopenia, the 2nd – 11 patients with presarcopenia, and the 3rd – 12 patients with sarcopenia. BMD was assessed by dual-energy X-ray absorptiometry in the neck and proximal femur as a whole and in the lumbar spine.Results. Osteopenic syndrome in the study cohort was diagnosed in 23 (71,9%) patients: osteopenia – in 19 (59,4%) patients, osteoporosis – in 4 (12,5%). Normal BMD values were observed in 9 (28.1%) patients. Osteopenic syndrome was detected in patients with sarcopenia signifcantly more often compared to patients without sarcopenia (p > 0.05) and with presarcopenia (p = 0.05). BMD indices in patients with sarcopenia in the neck and proximal femur in general were statistically signifcantly lower than in patients without sarcopenia and with presarcopenia (p < 0.05). There was a direct correlation between total skeletal muscle area at the LIII level and densitometric indices of the femoral neck.Conclusions. Sarcopenia is associated with low BMD indices in the neck and proximal femur in general in elderly and senile male patients with knee OA
The Effect of Ball Milling Time on the Isolation of Lignin in the Cell Wall of Different Biomass
Authors: Yang Guangrong , An Xueying , Yang Shilong 
Published: December 2021

Ball milling technology is the classical technology to isolate representative lignin in the cell wall of biomass for further investigation. In this work, different ball milling times were carried out on hardwood (poplar sawdust), softwood (larch sawdust), and gramineous material (bamboo residues) to understand the optimum condition to isolate the representative milled wood lignin (MWL) in these different biomass species. Results showed that prolonging ball milling time from 3 to 7 h obviously increased the isolation yields of MWL in bamboo residues (from 39.2% to 53.9%) and poplar sawdust (from 15.5% to 35.6%), while only a slight increase was found for the MWL yield of larch sawdust (from 23.4% to 25.8%). Importantly, the lignin substructure of ß-O-4 in the MWL samples from different biomasses can be a little degraded with the increasing ball milling time, resulting in the prepared MWL with lower molecular weight and higher content of hydroxyl groups. Based on the isolation yield and structure features, milling time with 3 and 7 h were sufficient to isolate the representative lignin (with yield over 30%) in the cell wall of bamboo residues and poplar sawdust, respectively, while more than 7 h should be carried out to isolate the representative lignin in larch sawdust.
Lack of Association Between Helicobacter pylori Infection and the Risk of Thyroid Nodule Types: A Multicenter Case-Control Studyin China
Authors: Wang Xiao-Song , Xu Xi-Hai , Jiang Gang , Ling Yu-Huan , Ye Tian-Tian , Zhao Yun-Wu , Li Kun , Lei Yu-Ting , Hu Hua-Qing , Chen Ming-Wei , Wang Heng 
Published: December 2021

The prevalence of Helicobacter pylori infection is high worldwide, while numerous research has focused on unraveling the relationship between H. pylori infection and extragastric diseases. Although H. pylori infection has been associated with thyroid diseases, including thyroid nodule (TN), the relationship has mainly focused on potential physiological mechanisms and has not been validated by large population epidemiological investigations. Therefore, we thus designed a case-control study comprising participants who received regular health examination between 2017 and 2019. The cases and controls were diagnosed via ultrasound, while TN types were classified according to the guidelines of the American College of Radiology Thyroid Imaging Reporting and Data System (ACR TI-RADS). Moreover, H. pylori infection was determined by C14 urea breath test, while its relationship with TN type risk and severity was analyzed using binary and ordinal logistic regression analyses. A total of 43,411 participants, including 13,036 TN patients and 30,375 controls, were finally recruited in the study. The crude odds ratio (OR) was 1.07 in Model 1 (95% CI = 1.03–1.14) without adjustment compared to the H. pylori non-infection group. However, it was negative in Model 2 (OR = 1.02, 95% CI = 0.97–1.06) after being adjusted for gender, age, body mass index (BMI), and blood pressure and in Model 3 (OR = 1.01, 95% CI = 0.97–1.06) after being adjusted for total cholesterol, triglyceride, low-density lipoprotein, and high-density lipoprotein on the basis of Model 2. Control variables, including gender, age, BMI, and diastolic pressure, were significantly correlated with the risk of TN types. Additionally, ordinal logistic regression results revealed that H. pylori infection was positively correlated with malignant differentiation of TN (Model 1: OR = 1.06, 95% CI = 1.02–1.11), while Model 2 and Model 3 showed negative results (Model 2: OR = 1.01, 95% CI = 0.96–1.06; Model 3: OR = 1.01, 95% CI = 0.96–1.05). In conclusion, H. pylori infection was not significantly associated with both TN type risk and severity of its malignant differentiation. These findings provide relevant insights for correcting possible misconceptions regarding TN type pathogenesis and will help guide optimization of therapeutic strategies for thyroid diseases.
Seasonal and Inter-Annual Variations of Stable Isotopic Characteristics of Rainfall and Cave Water in Shennong Cave, Southeast China, and Its Paleoclimatic Implication
Authors: Tian Ye , Zhang Haiwei , Zhang Rui , Zhang Fan , Liang Zeyuan , Cai Yanjun , Cheng Hai 
Published: December 2021

Speleothem calcite stable oxygen isotope (δ18OC) is one of the most widely used proxies in paleoclimate research, and understanding its seasonal-annual variability is very significant for palaeoclimate reconstruction. Five-year precipitation and karst cave water from 2016 to 2021 were monitored in Shennong cave, Jiangxi Province, Southeast China. The local meteoric water line (LMWL) is δD = 8.20 × δ18O + 13.34, which is similar to the global meteoric water line. The stable hydrogen and oxygen isotope (δD and δ18O) characteristics of precipitation and cave water were studied. δ18O and δD of precipitation and cave water show obvious seasonal variations. Lower precipitation δ18O and δD generally occur during summer and autumn compared with higher δ18O and δD values during winter and spring. Meanwhile, low precipitation δ18O values do not only appear in June–July when precipitation is the highest of the year but also appear in August–September when precipitation is limited. The back-trajectory analysis of monsoon precipitation moisture sources shows that the moisture uptake regions vary little on inter-annual scales; the water vapor of rainfall in June–July comes from the South China Sea and the Bay of Bengal, while the moisture source in August–September is mainly from the West Pacific and local area. The El Niño-Southern Oscillation is an important factor affecting the value of δ18O by modulating the percentage of summer monsoon precipitation in the annual precipitation and moisture source. The relationship between amount-weighted monthly mean precipitation δ18O and Niño-3.4 index shows that the East Asian summer monsoon (EASM) intensifies during La Niña phases, resulting in more precipitation in monsoon season (May to September, MJJAS) and lower δ18O values, and vice versa during El Niño phases.