bluuebunny/crossref_metadata_2025_split huggingface dataset:
PSIX-19 Effect of substituting groundnut cake with wheat bran mixed with cattle blood meal at ratio 1:1 on performance of broilers
Authors: Aderemi Foluke A 
Published: October 2021

Abstract
This study investigated substituting groundnut cake with wheat bran mixed with cattle blood at ratio1:1 in broilers and it lasted for eight weeks. Seventy-five broiler chicks were allotted randomly to five treatment replicated thrice. Control had 15% GNC, 0% blood meal wheat bran (BMWB) in diets II -V GNC was replaced with BMWB at graded levels of 25,50,75 and 100%. Results revealed that body weight gain and feed intake of diets II and III were similar and (P = 0.05) higher than other diets at both phases. Feed efficiency of diet II was better than others at the starter phase while diet III was better at finisher phase. Haematology and serum chemistry at the finisher phase showed that heamoglobin, white blood cell lymphocyte heterophil and plasma of the birds fed diets II, III, IV and V were (P &lt; 0.05) higher than those on control. The kidney liver and spleen histopathology revealed hepatic and renal degeneration, necrosis and inflammatory responses in diets IV and V. Carcass characteristics showed the wings shanks drumstick leg heart and spleen of birds on BMWB were higher than control. Conclusively it seems BMWB could replace GNC without adverse effect on the broilers at 25% inclusion level
PSX-B-24 Influence of the immunomodulatory drug Azoxivet on the post-vaccination immune response in laying hens
Authors: Sabrekova Valentina , Korenyuga Maxim , Konovalova Elena , Rodionova Natalia 
Published: October 2021

Abstract
Vaccination is a primary way to prevent infectious disease in poultry. The quality of immune response depends on the immune status of the body which, in turn, depends on many endogenous and exogenous factors. This study analyzed the effect of the immunomodulatory drug Azoxivet on the immune response in laying hens after vaccination against Newcastle Disease and Infectious Bursal Disease. There were 4 groups of Loman White cross laying hens (n=20). At age 120 days, all hens habitated in individual cages with an area of 0,15 m2. The conditions of keeping and feeding matched breed requirements. All groups were vaccinated against Newcastle Disease (NDV) (LaSota strain) and Infectious Bursal Disease (IBD) (Winterfield 2512 strain). All groups received Azoxivet (Az) at a dose of 0,3 mg/kg in water three times daily. Blood sera were collected weekly for serological studies using BioChek IBD and NDV test systems. The antibody level of all hens before and after vaccination: 1378 (IBD+Az) vs. 1674,93 (IBD) (P &lt; 0,05), 5194,75 (NDV+Az) vs. 5612,87 (NDV) (P &lt; 0,05). After one week of vaccination: 5931,25 (IBD+Az) vs. 5006,14 (IBD) (P &lt; 0,05), 6207,58 (NDV+Az) vs. 5765,21 (NDV) (P &lt; 0,05). Two weeks: 11086,00 (IBD+Az) vs. 10485,83 (IBD) (P &lt; 0,05), 11255,25 (NDV+Az) vs. 8478,75 (NDV) (P &lt; 0,05). Three weeks: 11478 (IBD+Az) vs. 8286 (IBD) (P &lt; 0,05), 14725 (NDV+Az) vs. 12677 (NDV) (P &lt; 0,05). Four weeks: 12999 (IBD+Az) vs. 1009,67 (IBD) (P &lt; 0,05), 17399 (NDV+Az) vs. 16373,17 (NDV) (P &lt; 0,05). Five weeks: 13601,15 (IBD+Az) vs. 9021,30 (IBD) (P &lt; 0,05), 19671 (NDV+Az) vs. 16309 (NDV) (P &lt; 0,05). Thus, Azaxul had a positive influence on the post-vaccination immune response in laying hens when used after Newcastle Disease and Infectious Bursal Disease vaccines. The results of the study can be used to improve disease prevention in poultry farming.
PSX-B-21 Diagnosis of early mastitis in dairy cows: Somatic cells and bacterial pathogen measuring
Authors: Artemieva Olga , Nikanova Daria A , Kositsin Alexander , Lashneva Irina , Ignatieva Larisa P , Sermyagin Alexander A , Zinovieva Natalia A 
Published: October 2021

Abstract
Mastitis is an inflammatory condition of the breast that can be caused by chemical, physical, traumatic injury or bacteria. In recent years, biomarkers for the mastitis diagnosis have been actively studied. The aim of the study was to assess the relationship between SCC and pathogenic bacteria in milk occurrence. The study carried out by milk samples collected in the experimental herd (Krasnodar region, Russia) from 85 Holsteinized Black-and-White cows. Healthy cows (HC; n = 4), cows at risk for clinical mastitis (RCM, n = 22), subclinical (SCM; n = 25) and clinical mastitis (CM; n = 34) groups were divided by a bacteriological cultivation and SCC level (Fossomatic7 DC). SCC results were logarithmically converted to SCS. Consequent groups were included animals with SCS level: HC (SCS &lt; 4.1), RCM (4.2≤SCS≤5.3), SCM (5.4≤SCS≤6.3), CM (SCS &gt;6.4). HC group of animals showed SCS equal to 3.11±0.28, 4.85±0.44 for RCM, 5.8±0.28 for SCM and 7.43±0.87 for CM. Identification of isolated bacteria species was carried out by conventional biochemical methods using the API20E, APIStaph, API20Strep tests (bioMerieux SA,France). Number of isolated pathogenic bacteria in CM group was 57 strains of which 33.3% were attributed to Enterobacteriaceae, 45.6% to coagulase-negative staphylococci (CONS), 12.3% to S.aureus and 8.8% to Ps.aeruginosa. In SCM group there were assigned 48 strains: 32.5% to Enterobacteriaceae strains, 42.5% to CONS, 20.0% to S.aureus, 5.0% to Ps.aeruginosa. The antibiotic susceptibility was determined according to NCCLS and EUCAST. S.aureus isolates showed the highest sensitivity to erythromycin (4.3% of resistant strains) and the highest resistance to ciprofloxacin (100%), tetracycline (95.0%), rifampicin (88.5%), benzylpenicillin (79.3%), novobiocin (69.2%) and fusidin (65.5%). The primary analysis of SCC and milk microbiological profile can be able to increase the accuracy of mastitis occurrence diagnosing that contributes to taking the right decision for choosing an antibiotic to preserve the cows’ health in Russian cattle population. The study was funded by RSF (project No.21-76-20046)
PSI-4 Animal and agronomic performance while grazing winter stockpiled novel endophyte tall fescue established with three pasture renovation strategies
Authors: Talbott Charlotte L , Harmon Deidre D , Poore Matt H , Franluebbers Alan J , Young Carolyn A , Griffith Emily H 
Published: October 2021

Abstract
Comparing animal and agronomic performance following renovation of toxic-infected tall fescue (TF) to novel endophyte tall fescue (NE) is fundamental to evaluating economic returns and increasing adoption of NE. In 2018, three NE renovation strategies were implemented in a randomized complete block design in Bahama, NC. Strategies included: 1) control (C), 2) renovation to NE after one season of a single specie cover crop (1-SM), 3) renovation to NE after three seasons of a single specie cover crop (3-SM), and 4) renovation to NE after three seasons of a multi-specie cover crop (3-CM). Sixty-four Angus heifers (initial BW 290 kg ± 88) were randomly assigned to treatments and strip-grazed stockpiled fescue for 56-d, two years after NE establishment. Forage yield and nutritive value was measured every 14-d. Data were analyzed using proc GLIMMIX of SAS v9.4. Average daily gain was greatest (P = 0.0024) for cattle grazing 1-SM (0.67 kg/d), and least for 3-CM (0.54 kg/d) and C (0.44 kg/d), which did not differ. 3-SM (0.57 kg/d) did not differ from other treatments. Within the grazing period, forage yield was greatest (P = 0.0001) in 3-CM, 3-SM, and C compared to 1-SM (3053, 2941, 2856 and 2465 kg/ha, respectively). Prior to the initiation of grazing, crude protein concentration was greater (P = 0.0089) for 3-CM (17.5%) compared to C, 1-SM, and 3-SM (15.8%, 15.8% and 15.3%, respectively). Neutral detergent fiber was greater (P = 0.0135) for 3-SM (57%) than C (55.1%) and 3-CM (54.5%), but did not differ from 1-SM (54.6%). Concentration of total digestible nutrients was greater (P = 0.0187) for 3-CM (73.2%) compared to 1-SM (72.1%) and 3-SM (71.9%), but did not differ from C (72.5%). Data suggest animal and agronomic performance for NE was improved compared to TF during winter stockpile grazing in years following renovation.
PSI-15 Embryo viability based upon energy
Authors: Hickerson Sydney L , Wells Cara W , Jesko Noah P , Perkins Thomas L , Killingsworth Russell 
Published: October 2021

Abstract
The objective of this study was to analyze embryo energy release and whether or not it is indicative of survivability upon successful implantation. In both bovine and equine reproduction, fertility is a lowly heritable trait that has an exceedingly large financial impact on the ability to grow genetic lines. In order to increase pregnancy rates, producers must have access to accurate decision-making tools. Nineteen (n = 19) fertilized bovine embryos collected via flushing by licensed veterinarian, Dr. Russell Killingsworth, DVM, in Shamrock, TX, were assessed for energy release using amplification of video with a Nikon Camera and Iolight Microscope. Once embryos were flushed, they were filmed for a thirty-five (35) second observation period. Embryo dimensions were then taken using Image J software. Embryo release of energy was humanly perceptible and measurable by changes in their morphological dimensions. Measurements of significance were taken on the embryo intercellular mass area (ICM), total embryo area, and on the vertical, diagonal, and horizontal axis of the sub-zonal distance, or the distance between the zona pellucida and outer edge of the ICM. All measurements were taken in microns. Once embryos were successfully measured, all were transferred into recipients. Significant findings presented that embryos with a greater ICM, total embryo area, and sub-zonal vertical, diagonal, and horizontal distance established pregnancies more frequently than embryos with less momentous differences in distance (P &lt; 0.05). This research has shown the relationship between embryo energy and pregnancy had a significance of determining pregnancy viability. This has therefore resulted in enhanced prediction of embryo transfer techniques based on embryo quality making pregnancy rates for producers more predictable and consistent.
PSI-11 Effects of a supplemental water source and trace-mineral based electrolyte drinking solution on intake and blood cell count of newly received feedlot calves
Authors: Smithyman Mackenzie M , Gouvêa Vinícius N , Campbell Dayna L , Duff Glenn C , Branine Mark E 
Published: October 2021

Abstract
Oral hydration therapy has been used to improve performance and health of newly received feedlot calves; however, little is known regarding water intake (WI) following arrival at the feedlot. Our objective was to evaluate WI of newly received feedlot calves provided a supplemental water source or a novel nutritional rehydration solution during initial 3 days following arrival. Crossbred heifers (n=180; initial BW = 237 ± 23 kg) were individually weighed after 16 h fasting and sorted into 12 pens (4 pens/treatment). Treatments were: 1) Control (CON): water provided through standard in-pen automatic waterer only (Richie CM480; one waterer/pen); 2) Supplemental water (SUPW): CON + water provided with one additional stock tank/pen; 3) Novel nutritional rehydration solution (NRS): trace-mineral based drinking solution provided with one stock tank/pen as the only water source. Treatments were provided from days 0 to 3 after which supplemental tanks were removed. From days 4 to 14 all heifers had access to the standard in-pen automatic waterer only. The WI was measured daily throughout the trial and BW was recorded at days 0 and 14. Whole blood was collected (5 heifers/pen) on days 0, 3, and 14. Treatments had no effect on DMI or ADG (P ≥ 0.15). SUPW and NRS had greater WI than CON from days 0 to d 3 (P ≤ 0.001), but not from days 4 to 14 nor from days 0 to 14 (P = 0.69). No treatment effect or treatment × day interactions were observed for total red (RBC) or white blood cell counts (WBC; P ≥ 0.19); however, a day effect was present (P &lt; 0.001) and RBC and WBC linearly decreased from day 0 to 14 (P &lt; 0.05). Our preliminary results indicate that providing a supplemental source of water during the initial 3 d after arrival increased total WI and may facilitate rehydration in stressed calves following transit.
PSI-5 Changes in the expression of MyD88, TRAF6, NFKB1, and NFKB1a on digestive tracts of 0 to 42 days-old calves from the tropics of Mexico
Authors: Pérez Mariana Álvarez , Robles-Rodriguez Carolina , Dávalos Laura González , Shimada Armando , Varela Alfredo , Izaguirre Ofelia Mira 
Published: October 2021

Abstract
During the first and second weeks of life, calves are extremely susceptible to enteric diseases, their digestive tract is developing and their thermoregulation is not adequate; these being some factors that can contribute to the lack of their weight gain (Hulbert & Moisá, 2016). In addition, they have a reduced capacity to generate an innate and adaptive immune response, in other words, they are still not immunocompetent (Costa et al., 2017; Hulbert & Moisá, 2016), since they are born with all the essential immune components but many of them are not functional until calves are at least 2 to 4 weeks old (Chase et al., 2008). With the aim to identify the development of the innate immune response through the expression of MyD88, TRAF6, NFKB1, and NFKB1a mRNA, in different ages and regions of the digestive tract, qPCR tests were held. Twelve Zebu crossbred calves of 0, 7, 28, and 42 days of age were sampled (three animals of each age). Tissue samples included: rumen, duodenum, and ileum. Gene expression was determined by quantitative PCR (qPCR). Changes in the expression of TRAF6, NFKB1 and NFKB1a mRNA were different in rumen and ileum (P &lt; 0.05) at day 28 of age. MYD88 mRNA expression was different only in ileum (P &lt; 0.05). MyD88, TRAF6, NFKB1, and NFKB1a mRNA expression of rumen tended to decrease and in the case of ileum to increase with age; however, this was until day 28 and not in the first week of life, as mentioned by some other authors.
PSIII-13 The Effect of the inclusion of liquid brewers yeast during gestation on colostrum quality and passive transfer of immunity in lambs
Authors: Navarrete Julissa , Earing Jennifer E , Holder Mieke , Rickard Justin W , Lugar Drew W W 
Published: October 2021

Abstract
Fourteen Suffolk/Suffolk x Hampshire pregnant ewes were utilized to evaluate the effects of liquid brewers yeast on colostrum quality and the transfer of passive immunity in their offspring. Parainfluenza type 3 (PI3) vaccination was used elicit an immune response. Ewes were vaccinated with PI3 at 33 and 18 d prior to expected lambing day. Ewes were randomly assigned to one of two treatments: late gestation diet (CON) or late gestation diet supplemented with liquid brewers yeast at an average rate of 60 g DM/d (LBY). Ewes were fed treatment diets from 45 d prior to expected lambing to 3 d post lambing. Blood was collected from ewes’ via jugular venipuncture prior to vaccination and within 6 hours postpartum. Blood was collected also from the jugular vein of lambs (n = 22) at 3 d of age. Ewe and lamb blood serum was tested for Bovine Parainfluenza 3 Virus and data on anti-PI3 antibody titers was evaluated. Colostrum was collected within 4 hours postpartum and analyzed for colostral IgG concentrations using a refractometer in % Brix. A PROC GLM model was performed in SAS 9.4, where the model for ewe colostrum quality and lamb anti-PI3 titers analyses included the main effect of treatment diet. All vaccinated ewes were positive for PI3 antibodies (&gt;64 titer) immediately prior to and post lambing. All lambs tested positive for antibody titers for PI3 with no differences between treatment (197.3 for CON and 160.8 for LBY; P = 0.43). The colostral IgG concentrations did not differ between CON (30.4% Brix) and LBY (24.6% Brix) ewes(P = 0.21). These results indicate that, although there were numerical differences, the addition of LBY to the ewes’ diet during late gestation did not significantly improve colostrum quality or passive transfer of immunity in lambs.
PSIII-1 Influence of prenatal or postnatal exposure to gossypol from cottonseed meal on semen quality in commercial boars: a preliminary study on feral hog control
Authors: Benefield Randi F , Mudarra Richard A , Tsai Tsung-Cheng Cheng , Hansen Christopher R , Maxwell Charles V , Rorie Rick W , Littlejohn Brittni P 
Published: October 2021

Abstract
The objective was to examine the influence of prenatal (Experiment 1) or postnatal (Experiment 2) exposure to gossypol from cottonseed meal (CSM) on semen quality in commercial boars. In Experiment 1, pregnant sows (n = 5) were fed a diet containing 0% (n = 1), 0.04% (n = 2), or 0.08% (n = 2) gossypol between d 56 and 86 of gestation. Boars (n = 11) born to sows in each treatment group (0% gossypol n = 3; 0.04% gossypol n = 4; 0.08% gossypol n = 4) were fed a common diet without CSM, and semen was collected at 269±2 d of age using a live sow in estrus. In Experiment 2, boars (n = 21) were fed a diet containing 0%, 0.02%, or 0.04% gossypol between 63±1 and 105±1 d of age (Initial BW: 19.85±0.43 kg). After the treatment period, boars were fed a common diet without CSM, and semen was collected at 238±7 d of age using a breeding dummy. Sperm cell concentration, percentage of motile sperm cells, and percentage of progressively motile sperm cells were analyzed using the MIXED procedure of SAS with treatment as a fixed effect in Experiment 1 and 2 and dam as a random effect in Experiment 1. In Experiment 2, semen was not successfully collected from every boar; therefore, chi-square analysis was used to assess semen collection status between treatment groups using the FREQ procedure of SAS. In Experiment 1, there was no difference in sperm concentration (P = 0.45), percent motility (P = 0.71), or percent progressive motility (P = 0.27) between treatment groups. In Experiment 2, there was no difference in sperm concentration (P = 0.72), percent motility (P = 0.17), or percent progressive motility (P = 0.87) between treatment groups. No difference was observed in boar collection status between treatment groups (P = 0.77). In conclusion, prenatal or postnatal exposure to gossypol from CSM did not influence semen quality in commercial boars.
PSIII-14 The influence of dietary barley supplementation on American Wagyu feedlot performance
Authors: Tangredi Briana V , Thorndyke Meghan P , Guimaraes Octavio , Barrington Rebecca K , Borgerding Shaniece M , Thomas Tyler A , Hallmark Harrison , Yi Loh Huey , Gifford Ryan J , Levenson Jonah R , Engle Terry E 
Published: October 2021

Abstract
Eighty-nine American Wagyu steers were used to evaluate the effects of dietary barley supplementation on feedlot performance. We hypothesized that barley supplementation would increase ADG compared to non-supplemented control animals. The experimental design was a randomized complete block design. Steers were blocked by initial body weight (BW) and randomly assigned within block to one of two treatments. Treatments consisted of 1) Control (no supplemental barley) and 2) Control diet + 0.9 kg∙animal-1∙d-1 of supplemental barley. Steers were housed in feedlot pens (all pens contained n = 4 steers/pen with the exception of one Control pen that contained n=5 steers; 11 replicates/treatment; experimental unit = pen) and fed a traditional American Wagyu finishing diet (DM basis: 68.42% DM, 14.33% CP; TDN: 74.76%, NEg: 1.16 Mcal/kg, 5.25% EE) for 270d. The basal diet consisted of grass hay, corn silage, cracked corn grain, soybean meal, corn distillers grain, white salt, ground limestone, and olive byproduct. Diets were fed once daily in the morning and barely was top dressed to the appropriate pens, immediately after the basal diet was delivered. Steers were individually weighed on d -1 and 0, and approximately every 28 days throughout the 270d experiment. Data were analyzed using a mixed effects model (PROC MIXED, SAS) for a completely randomized block design. Initial pen BW was used as a covariate in the statistical analysis of all performance data and significance was determined at P ≤ 0.05. Initial and final BW, ADG, DMI, and feed:gain were similar across treatments. Therefore, under the conditions of this experiment, these data suggest that barley supplementation for 270d to American Wagyu cattle did not impact overall animal performance.
PSIII-12 The effect of maternal dietary isoflavone supplementation and infection with porcine reproductive and respiratory syndrome virus on the growth performance of offspring
Authors: Chen Xuenan 
Published: October 2021

Abstract
The objective of this experiment was to evaluate the effects of maternal infection with porcine reproductive and respiratory syndrome virus (PRRSV) on piglet prenatal development and pre-weaning growth, and the potential for isoflavone supplementation to alleviate these effects. First parity gilts (n = 24) at gestational day (GD) 65 were allotted to one of three treatments: uninfected and fed a diet devoid of isoflavones (CON), infected with PRRSV and fed the control diet (POS), or infected with PRRSV and fed a diet supplemented with 1,500 ppm soy isoflavones (ISF). Gilts were inoculated intranasally with saline or 2.5×104 TCID50/mL of suspended live PRRSV (NADC20 strain) on GD 70. To determine the effects of PRRSV infection in sows, feed intake (GD 70–114) and rectal temperatures (GD 70–91) were recorded daily, while serum TNF-α concentrations were measured weekly. After farrowing (GD 114 ± 2), two piglets closest to the average litter weight were culled either at birth or weaning (21 d of age) to determine body, muscle, and organ weights of offspring pigs. Hematoxylin and eosin staining was performed on left semitendinosus muscles for determination of muscle cell number and size at each time-point. At birth, whole body, muscle, and organ weights were not different (P &gt; 0.22) between groups except that relative liver weight was increased (P &lt; 0.05) in POS compared with CON piglets. At weaning, ISF reduced (P ≤ 0.05) whole body and muscle weights, but greater (P &lt; 0.05) kidney weight compared with CON, and greater (P &lt; 0.05) relative liver weight compared with CON or POS. However, muscle fiber number and size were not different (P &gt; 0.39) between groups at either time-point. These results suggest maternal PRRSV infection altered offspring organ growth, but dietary ISF did not alleviate the detrimental effects of PRRSV infection in sows or offspring.