bluuebunny/crossref_metadata_2025_split huggingface dataset:
Fertility-Sparing Treatment and Assisted Reproductive Technology In Patients With Endometrial Carcinoma And Endometrial Hyperplasia: Pregnancy Outcomes After Embryo Transfer
Authors: Friedlander Hilary , Blakemore Jennifer , McCulloh David , Fino M. 
Published: September 2021

Abstract
Purpose: To evaluate pregnancy outcomes following embryo transfer in patients with endometrial carcinoma (EMCA) or endometrial hyperplasia (EH) who elected for fertility-sparing treatment (FST). Methods: This retrospective cohort study at a large urban university-affiliated fertility center included all patients who underwent embryo transfer after fertility-sparing treatment for EMCA or EH between January 2003 and December 2018. Primary outcomes included embryo transfer results and a live birth rate (defined as number of live births per number of transfers).Results: There were 14 patients, 3 with EMCA and 11 with EH, who met criteria for inclusion with a combined total of 40 embryo transfers. An analysis of observed outcomes by sub-group, compared to the expected outcomes at our center (patients without EMCA/EH matched for age, embryo transfer type and number, and utilization of PGT-A) showed that patients with EMCA/EH after FST had a significantly lower live birth rate than expected (Z = -5.04, df =39, p < 0.01). A sub-group analysis of the 14 euploid embryo transfers resulted in a live birth rate of 21.4% compared to an expected rate of 62.8% (Z = -3.32, df = 13, p < 0.001).Conclusions: Among patients with EMCA/EH who required assisted reproductive technology, live birth rates were lower than expected following embryo transfer when compared to patients without EMCA/EH at our center. Further evaluation of the impact of the diagnosis, treatment and repeated cavity instrumentation for FST is necessary to create an individualized and optimized approach for this unique patient population
HER2 and p53 Expression in Marjolin’s Ulcer
Authors: chen xia , Gang Chu Zhi , May Jin Dong , Yan Xiao , Wen Ma 
Published: September 2021

Abstract
Background：Marjolin's ulcer is a malignant tumor that is different from other skin ulcers, and its pathogenesis is not yet clear. The diagnosis and prognosis of Marjolin's ulcer lack valuable marks on immunohistochemistry (IHC).Methods: In this study, we detected the expression of HER2 and p53 in Majorlin’s ulcer with immunohistochemistry, and retrospectively analyze the clinicopathological characteristics of Marjolin's ulcer samples.Results: Our results showed that no HER2 but p53 was detected in Majorlin’s Ulcer samples. Meanwhile, by statistic analysis we found that the positive expression rate of p53 in Majorlin’s Ulcer samples was associated with sex, course of disease, ulcer size, pathological type of ulcer canceration, and degree of tumor differentiation. Furthermore, we showed that the positive rate of p53 was proportional to the malignancy degree of Marjolin’s ulcer. Conclusions:Our results of this study will lay a foundation for diagnosis of Marjolin's ulcer and even prevention of Marjolin's ulcer progression
Analysis of the Influence of Sources on the Spatial Variation in Atmospheric Methane Concentrations in the City of Tandil, Argentina
Authors: Fusé Victoria Susana , Stadler Carla Sofia , Picone Natasha , Linares Santiago , Guzman Sergio Alejandro , Juliarena Maria Paula 
Published: September 2021

Abstract
There is an overall trend in urban methane (CH4) emissions due to the presence of several sources; however, differences exist between cities, and therefore further local research should be undertaken. The present study analyzes the spatiotemporal variation in atmospheric CH4 concentrations during a year at ten sampling sites in the urban core of a medium-sized city. The mean annual atmospheric CH4 concentrations varied between 2.02 ppm and 5.45 ppm; the maximum concentrations were found in a site close to a wastewater treatment plant (WWTP), presenting a significant increase toward the summer. In the rest of the sites, the maximum concentrations were recorded in the coldest months due to the influence of combustion sources dependent on natural gas (NG). An exploratory regression analysis was performed, in which the variables “homes connected to the gas network” and “distance from compressed NG stations” each explained 66 and 65% of the spatial variation of the atmospheric CH4 concentrations at the 9 sites (excluding that one nearest the WWTP). The results show the need to prevent NG leaks in all urban areas to reduce the emissions of this potent greenhouse gas, which, at the same time, will provide economic benefits for the sectors involved.
The Clinical Manifestation of Straight Back Syndrome in Echocardiogram Performance
Authors: Yu Wanqian , Zhang Hongzhou , Yang Pingping , Gan Lujin , Wang Chenxi , Zhu Dan , Yao Xiongda , Zhao Yuanbin , Wu Tao , Liu Yanna , Wu Qinghua 
Published: September 2021

Abstract
Background: There were no studies on the mechanism of clinical manifestations of straight back syndrome. Our aim was to explore the mechanism of clinical symptoms of straight back syndrome.Methods: From February 2018 to February 2021, we included 4 patients (3 males and 1 female) with straight back syndrome as the experimental group, and 4 normal people matched with sex and BMI as the normal control group. Basic information of patients, laboratory examination, echocardiography in supine and standing position, chest film in positive and lateral position, basic information of control group and echocardiography in supine and standing position were collected. The differences of clinical data between the two groups were compared and analyzed. Results: There were significant differences in left ventricular outflow tract diameter, right ventricular outflow tract diameter and right ventricular outflow tract velocity in the experimental group (their P values were 0.035, 0.011 and 0.015, respectively), but there was no significant difference in left ventricular outflow tract velocity in the standing and supine position (P=0.638). The left ventricular outflow tract diameter, the right ventricular outflow tract diameter and the right ventricular outflow tract velocity in the experimental group were significantly different (P<0.05). The internal diameters of left ventricular outflow tract in upright position, supine position, right ventricular outflow tract, supine position, right ventricular outflow tract were20.50±1.91mm, 18.75±0.96mm;10.00±6.68mm, 15.45±6.06mm;124.25±40.29cm/s and 91.00±28.93cm/s, respectively, in standing position, recumbent position, right ventricular outflow tract, right ventricular outflow tract, supine position, right ventricular outflow tract, supine position, supine position. The velocity of left ventricular outflow tract in upright position was 82.50±2.01 cm/s, and that in supine position was 83.25±2.06 cm/s. There was no significant difference in left ventricular outflow tract velocity in standing and supine position, which may be related to the compression of right ventricular outflow tract.Conclusions: In this study, we got the clinical manifestations of direct-back syndrome patients through comparison of straight back syndrome.
Insights Into The Effect of Shelter Modification On Key Biochemical Aspects vis-a-vis the Performance of Sahiwal Zebu Cows Reared in Hot Arid Ambience
Authors: Arora R. , Maan R. , Gujar G. 
Published: September 2021

Abstract
This study was undertaken to evaluate the effect of shelter modifications in the form of floor alteration and heat stress amelioration aids on the biochemical aspects and productive performance of Sahiwal zebu cows. 24 healthy Sahiwal cows in their second or third parity were randomly assigned to four groups (G1, G2, G3, and G4) having 6 cows each and were studied for duration of 150 days from June to November. G1 acted as control without any shelter modification, while G2 cows were housed in stalls with rubber mat covered floors, G3 cows were provided with cooling fans along with water sprinkling twice a day, and G4 cows were housed in stalls combining rubber mat floors with cooling fans and water sprinkling twice a day. This study revealed a significant (p<0.05) effect of shelter modification on milk yield, though no significant effect on milk composition was found. Among blood biochemical parameters, serum cholesterol and cortisol levels registered a significant (p<0.05) effect of shelter modification. The use of heat amelioration aids with, and without rubber mat floors positively influenced the productive and biochemical aspects of Sahiwal cows. Such strategies can be utilized to reduce stress on animals and help in maintaining their production.
Metabolic Engineering of Escherichia Coli BL21 Strain Using Simplified CRISPR-Cas9 and Asymmetric Homolog Arms Recombineering
Authors: Shukal Sudha , Lim Xiao Hui , Zhang Congqiang , Chen Xixian 
Published: September 2021

Abstract
BackgroundThe recent CRISPR-Cas coupled with λ recombinase mediated genome recombineering has become a common laboratory practice to modify bacterial genomes. It requires supplying a template DNA or homolog arms for precise genome editing. However, it is often overlooked the process to generate the homolog arms which is a time-consuming, costly and inefficient step.ResultsIn this study, we first optimized CRISPR-Cas protocol in BL21 strain and successfully deleted 10 kb gene from the genome in one round of editing. To further simplify the protocol, asymmetric homolog arms as PCR fragments was used. It can be obtained by one-step PCR reaction with two primers and purified with desalting columns. Unlike conventional homolog arms that are prepared through overlapping PCR, cloning to plasmid or annealing synthetic DNA fragments, our method significantly shortened the time taken and reduced the cost to prepare the homolog arms. To test the robustness of the optimized workflow, we successfully deleted 26 / 27 genes across BL21 genome. Noteworthy, gRNA design is important for CRISPR-Cas system and a general heuristic gRNA design was proposed in the study. To apply our established protocol, we targeted 16 genes and iteratively deleted 7 genes from BL21 genome. The resulting strain increased lycopene production from ~15,000 ppm to > 40,000 ppm.ConclusionsOur work has optimized the homolog arms design for gene deletion in BL21 strains. The protocol efficiently edited BL21 strain to improve lycopene production. The same workflow is applicable to all E. coli strain which would be useful for genome rewiring to further increase metabolite production in microbial cell factories.
A Novel Nomogram Combined With Radiomics Features, Age and Albuminuria to Predict the Pathological Grade of Bladder Cancer Running Title: Application Study on the Prediction Model of Bladder Cancer Pathological Grade Based on Parameters of Thin-layer Enhanced CT. Author Information
Authors: Zhou Qi , Ma Lu , Zhang Haoyang , Ang Xiaojie , Hu Can , Mao Caiping , Zhang Zhiyu , Ouyang Jun 
Published: September 2021

Abstract
Background: Based on multi-parameter thin-slice enhanced CT texture features and related clinical indicators, a preoperative pathological grade prediction model of bladder urothelial carcinoma was established.Methods: The CT images and clinical data of 372 patients with urothelial carcinoma in our hospital from January 2015 to October 2020 were collected. 372 patients were divided into two groups: HGUC(n=190) and LGUC(n=182). All patients were divided into 10 groups on average, of which 7 were used as training group (n=259) and the remaining 3 as verification group (n=113). Then, by using 3D-Slicer software from all enhanced in patients with preoperative CT images (Arterial and Venous phase calibration chart) split out the region of interest (ROI), respectively from the tumor image data extraction based on First-order and Second-order, High-order and filtering characteristics of 1223 texture features, and use the inter/intra-class correlation coefficient(ICC > 0.75) between classes and least absolute shrinkage selection operator (LASSO) regression feature selection; Secondly, the clinical effective factors were obtained by logistic regression analysis, and the clinical predictive model was constructed. Finally, the selected clinical key indicators and radiomics features were mapped. In order to verify the predictive ability of the nomogram, conformance index (C-index), calibration curve, Receiver operator characteristic (ROC) curve and clinical decision curve analysis (DCA) were used to test the nomogram.Results: Lasso regression analysis showed that 11 radiomics features were significantly correlated with the pathological grade of bladder cancer. After comparing the four models, it is found that Logistic regression model has the best prediction ability (AUC=0.858). The results of multivariate analysis showed that age and albuminuria were independent influencing factors. A comprehensive model for predicting the pathological grade of bladder cancer (radiomics + clinical) was constructed by combining clinical independent risk factors with 11 radiomics features. Compared with clinical feature model and radiomics model, it was found that the predictive performance of imaging comprehensive model combined with clinical factors was the best (AUC=0.864).Conclusions: The radiomics model based on multi-parameter thin-layer enhanced CT, combined with clinical factors, can effectively predict high-and low-grade urothelial carcinoma.
Comparison of Efficacy Between Triamcinolone Acetonide and Triamcinolone Hexacetonide for Intraarticular Therapy in Juvenile Idiopathic Arthritis: A Retrospective Analysis
Authors: De Ranieri Deirdre , Chun Angela , Muhammad Lutfiyya 
Published: September 2021

Abstract
Background

There are many FDA-approved corticosteroid preparations available for intra-articular injection, however triamcinolone hexacetonide is not one of them. It was the intraarticular drug of choice among pediatric rheumatologists up until approximately a decade ago, when production of this medication ceased. It can be obtained in the United States and Canada via importation from Europe, but it is not FDA-approved at this time. We wish to compare the duration of remission of intraarticular triamcinolone hexacetonide (TH) with that of triamcinolone acetonide (TA) in children with Juvenile Idiopathic Arthritis (JIA) and demonstrate its safety in this population.
Methods

This retrospective chart review included 39 patients with JIA who received intraarticular corticosteroid injections (IACIs) from September 2018 to September 2019. These patients were reviewed and their life-time injections with either TH (41 joints) or TA (124 joints) was noted through May 30, 2021. Patients with concomitant systemic therapy initiation were excluded. The primary outcome was time to relapse. Relapse was defined by the presence of arthritis on physical examination by an attending rheumatologist. Kaplan-Meier curves and a log-rank test were constructed to compare the probability of time to relapse between IACI injections. Additionally, mixed effects cox regression models were constructed to account for multiple injections per participant.
Results

Kaplan-Meier estimator of median relapse time in months was higher for TH. Based on the log-rank test, TA joints had a higher probability of experiencing a relapse during the study time (p-value < 0.001). The hazard of time to relapse was reduced when comparing TH to TA in both unadjusted and adjusted mixed effects cox regression models (unadjusted hazard ratio (95% confidence interval): 0.184 (0.089, 0.381); adjusted hazard ratio (95% confidence interval): 0.189 (0.092, 0.386)).
Conclusions

TH has longer duration of action than TA and is associated with less systemic side effects. It should be considered the drug of choice for intraarticular corticosteroid injections in children with JIA.
Construction of Co-Expression Modules Related to Survival by WGCNA and Identification of Potential Prognostic Biomarkers in Liver Cancer
Authors: Wang Tian , Zhang Lin-li , Feng Qing-hui , Wang Meng-ling , Fan Yu-Chun , Wang Zhuan , Deng You-lin , Jiang Li-He 
Published: September 2021

Abstract
Background Many recent studies have reported the role of WGCNA in liver cancer, and screened out modules related to the clinical characteristics of liver cancer. The prognostic role of modular genes provides a reference for the treatment of liver cancer and the study of survival time extension. MethodsUse edgeR to screen differentially expressed genes, and use weighted gene co-expression network analysis (WGCNA) method to divide differential genes into modules; combined with clinical data, select gene modules that are highly related to clinical information to construct a prognostic model. Use univariate Cox and multivariate Cox regression analysis to predict the value of prognosis. The Kaplan-Meier method and ROC curves were used to evaluate the value of features of predicting prognosis.ResultsIn the co-expression analysis of liver cancer, the two gene modules darkrey and navajowhite2 was highly correlated with the TNM stag of liver cancer. In addition, they were related to the reduction process and the activation of specific DNA-binding transcription factors. Constructing a risk ratio model with these two modules shows that the overall survival time of patients in the high-risk group is lower than that of the low-risk group. In the module, ABLIM2, RP11-109J4.1, TXNRD1, C9orf106, LINC00677, CYP11B2, AC005550.3 and MIR3945HG belong to the Hub gene, and can also be used as independent prognostic factors, which may become potential biomarkers for the diagnosis and treatment of liver cancer. Conclusion This studies identified and screened the prognostic genes RP11-109J4.1, TXNRD1, C9orf106, LINC00677, CYP11B2, AC005550.3 and MIR3945HG related to the progression of liver cancer, which can provide a reference form the diagnosis and treatment of liver cancer.
SMILE After DALK to Reduce Residual Refraction: Two-Year Results
Authors: Hashemi Hassan , Aghamirsalim Mohammadreza , Shahhoseini Saied , Moghaddasi Alireza , Asgari Soheila 
Published: September 2021

Abstract
PurposeTo determine the two-year results of small incision lenticule extraction (SMILE) for correcting post-keratoplasty myopia and myopic astigmatism.MethodsIn this case-series study, 10 eyes of 10 patients with a 6-10-year history of successful deep lamellar keratoplasty (DALK) underwent SMILE using VisuMax laser platform. Ophthalmologic examinations and visual acuity and refraction measurement were done pre- and 1, 3, 6, 12, and 24 months postoperatively. Pentacam and Sirius imaging was done in the first and last follow-up session.ResultsThe mean age of the patients was 39.60±7.86 years. Six subjects were male. After two years, uncorrected distance visual acuity did not change in one eye and improved 1-6 lines in other eyes. Corrected distance visual acuity decreased in one eye, did not change in four eyes, and improved 1-8 lines in other eyes. The mean decrease of spherical equivalent, spherical error, and cylinder power was 1.92±1.96 diopter (D) (P=0.013), 0.70±3.05D (P=0.213), and 2.42±2.91D (P=0.024), respectively. The vector mean target induced astigmatism, surgical induced astigmatism, and difference vector was 1.30D@44˚, 1.11D@24˚, and 0.86D@73˚, respectively. Two years after surgery, vertical coma, horizontal coma, and spherical aberration increased by 0.44±0.51, 0.23±0.32, and 0.02±0.16µm respectively (all P>0.05) while trefoil reduced by 0.29±0.75µm (P=0.428).ConclusionSMILE is a successful procedure for reducing refraction and astigmatism after DALK in patients with moderate myopia and moderate to severe astigmatism and improves the visual acuity in these patients. Axis rotation during surgery may result in under-correction of astigmatism. Refinement of SMILE treatment nomogram for post-DALK cases seems necessary.
The Mechanism Revealing and Law Explorating for the Nonlinear Response of Blade Disk Rotor System Under the Coupling Effects of Crack and Aerodynamic Force
Authors: Yang Jinsong , Xie Jingsong , Wang Tiantian , Yang Fei , Chen Jinglong 
Published: September 2021

Abstract
Blade disk rotor system is a typical structure of industrial equipments such as aeroengines and gas turbines. The research on the response characteristics and mechanism of the system under the coupling effects of aerodynamic force and blade crack is of great significance to the interpretation of vibration phenomena and diagnosis of faults. From the numerical solution based response characteristic analysis to the kinematics and dynamics based essential response mechanism revealing, from the model based special case study to the Number Theory based general law establishing, in this paper, the response mechanism of blade disk rotor system under the coupling effects of crack and aerodynamic force is studied comprehensively and deeply. Firstly, a simplified dynamic model of typical blade disk rotor system is constructed by using the classical continuous parameter modeling method. Based on the dynamic model, for two structural forms of moving and stationary blades, the typical characteristics of vibration response under the actions of aerodynamic force and blade crack are analyzed by means of numerical solution. Then, from the perspective of kinematics and dynamics, the internal mechanism between the vibration responses and the excitations are revealed. Finally, based on Number Theory, the response characteristics and mechanism of typical structures are summarized, and the general laws of responses with general structural forms are established.
Spinal Instrumentation Failure from the Perspective of Load Sharing
Authors: Kato Takaya , Ishikawa Santo , Inaba Tadashi , Kasai Yuichi , Paholpak Permsak , Wisanuyotin Taweechok , Sirichativapee Winai , Kosuwon Weerachai , Nabudda Kreingkrai , Mizuno Tetsutaro 
Published: September 2021

Abstract
Background In recent years, pedicle screw (PS) fixation has often been used to stabilize the spine and correct deformities, yielding good clinical results. On the other hand, PS fixation is known to show problems such as instrumentation failure. However, few biomechanical studies have described causes of instrumentation failure. In this study, causes of instrumentation failure in lumbar PS fixation were investigated from the perspective of load sharing by measuring both strains generated in the rod and intradiscal pressure.Methods Four human cadaveric multi-segmental lumbar vertebrae (L2-L5) were used to prepare a control model and a PS fixation model. Next, axial compression tests were performed on each model using a universal material testing machine, and the strains generated in the rods and the intradiscal pressures were measured from strain gauges attached to the rods and a pressure sensor installed between L3-L4.Results Combined compressive and bending stresses were found to be generated in the rod, with bending stress around 10 times higher than compressive stress, and with vast differences in the levels of stress generated between right and left rods. Moreover, the stress shielding by PS fixation was small, and intervertebral discs were still subjected to a large load. Conclusion Preventing instrumentation failure in lumbar spine PS fixation, it seems necessary to strengthen the durability of the rod against bending stress and to ensure the stability of the anterior stabilizing element of the spine.