bluuebunny/crossref_metadata_2025_split huggingface dataset:
Blebbistatin protects iPSC-CMs from hypercontraction and facilitates automated patch-clamp based electrophysiological study
Authors: Li Wener , Luo Xiaojing , Ulbricht Ying , Guan Kaomei 
Published: June 2021

AbstractRecently, there have been great advances in cardiovascular channelopathy modeling and drug safety pharmacology using human induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs). The automated patch-clamp (APC) technique overcomes the disadvantages of the manual patch-clamp (MPC) technique, which is labor intensive and gives low output. However, the application of the APC platform is still limited in iPSC-CM based research, due to the difficulty in maintaining the high quality of single iPSC-CMs during dissociation and recording. In this study, we improved the method for single iPSC-CM preparation by applying 2.5 μM blebbistatin (BB, an excitation-contraction coupling uncoupler) throughout APC procedures (dissociation, filtration, storage, and recording). Under non-BB buffered condition, iPSC-CMs in suspension showed a severe bleb-like morphology. However, BB-supplement led to significant improvements in morphology andINarecording, and we even obtained several CMs that showed spontaneous action potentials with typical morphology. Furthermore, APC faithfully recapitulated the single-cell electrophysiological phenotypes of iPSC-CMs derived from Brugada syndrome patients, as detected with MPC. Our study indicates that APC is capable of replacing MPC in the modeling of cardiac channelopathies using human iPSC-CMs by providing high-quality data with higher throughput.
Photoreceptor Compartment-Specific TULP1 Interactomes
Authors: Ebke Lindsey A. , Sinha Satyabrata , Pauer Gayle J.T. , Hagstrom Stephanie A. 
Published: June 2021

AbstractPhotoreceptors are highly compartmentalized cells with large amounts of proteins synthesized in the inner segment (IS) and transported to the outer segment (OS) and synaptic terminal. Tulp1 is a photoreceptor-specific protein localized to the IS and synapse. In the absence of Tulp1, several OS-specific proteins are mislocalized and synaptic vesicle recycling is impaired. To better understand the involvement of Tulp1 in protein trafficking, our approach was to physically isolate Tulp1-containing photoreceptor compartments by serial tangential sectioning of retinas and to identify compartment-specific Tulp1 binding partners by immunoprecipitation followed by liquid chromatography tandem mass spectrometry. Our results indicate that Tulp1 has two distinct interactomes. We report the identification of: 1) an IS-specific interaction between Tulp1 and the motor protein Kinesin family member 3a (Kif3a), 2) a synaptic-specific interaction between Tulp1 and the scaffold protein Ribeye, and 3) an interaction between Tulp1 and the cytoskeletal protein Microtubule-associated protein 1B (MAP1B) in both compartments. Immunolocalization studies in the wild-type retina indicate that Tulp1 and its binding partners co-localize to their respective compartments. Our observations are compatible with Tulp1 functioning in protein trafficking in multiple photoreceptor compartments, likely as an adapter molecule linking vesicles to molecular motors and the cytoskeletal scaffold.
How perception and attention participate in reading skill?
Authors: Gudwani Sunita , Mehta Manju , Sagar Rajesh , Behari Madhuri , Narang Vaishna , Dwivedi Sadanad , Jagannathan N.R. , Kumaran S. Senthil 
Published: June 2021

AbstractReading creates accessibility to the outer world information and is an integral skill for academic achievement. It involves visual perception, reasoning at symbolic level (text reading) and phonological processing. But does perception, visuospatial processing at pre-symbolic level affect reading skill? To understand and untangle the mechanism fifty children with reading problem (developmental dyslexia, DDC) and twentyfive age-matched typical readers (healthy controls, HC) were studied. In DDC, the variable-performance of non-symbolic visual search (pictorial-level) was associated with bilateral brain activity (functional MRI) of ventral stream (inferior occipital and fusiform) and greater involvement of frontal-prefrontal regions, suggesting the modified dorsal route-gating for figure-ground filtering. Performance variability in picture-concept reasoning (71.9% and significant group mean difference) and visuospatial-organization (51.3%) of DDC children indicate attentional or executive differences at nonsymbolic level. In symbolic discrimination performance, no significant difference observed for single alphabet or number (digit) reading but significant differences at word level for visual and phonological performance (considering orthographic differences), attributes to distractor-inhibition problems. Nonsymbolic to symbolic coding/decoding speed and writing error (copying) differences are suggestive of execution variability rather than motor coordination in DDC. In HC, the top-down dorsal route gating and hypoactivity induced in right frontal-anterior cingulate cortices, play a major role in reading by allocating spatial attention and inhibiting distractors. It has been elaborated in the “neurobiological model”.How perception and attention participate in reading skill?Research HighlightsVisuospatial attention, executive control (nonsymbolic level) and visual discrimination (symbolic level) performance contribute to reading.In children with reading problems (dyslexia) pictorial visual processing task (fMRI) recruited higher activation in right hemispheric frontal, inferior parietal and anterior cingulate regions.Bilateral activation of occipital-striate, extra-striate, fusiform for nonsymbolic is associated with significantly more errors at bigger-symbol-string, in dyslexia.Auditory discrimination differences in shallow-deep orthography.Correlation of non-symbolic executive and symbolic performances.Role of the prelexical visual perception and dorsal-route gating in reading skill explained as neurobiological model.
Foot scales in the Early Cretaceous bird Gansus yumenensis from China
Authors: Zhao Tao , Li Zhiheng , Zhang He , Pan Yanhong 
Published: June 2021

AbstractMost modern birds have scales covering the foot and feathers elsewhere. Discoveries of fossil feathers attached to the metatarsus in non-avian dinosaurs and basal birds suggests that the avian scales are secondarily derived from feathers. However, our knowledge of early avian scales and their taphonomy is still limited, due to the scarcity of fossil record. Here we employ multiple techniques to characterize the morphological and chemical details preserved and investigate how they are preserved in the skin of IVPP V15077, a referred specimen of the Early Cretaceous Gansus yumenensis. Results show that two types of scales, scutellate and interstitial scales, are preserved in IVPP V15077, which, in combination with previous discovery of scutate and reticulate scales in other Early Cretaceous birds, indicates that all four types of scales present in modern birds have appeared in the Early Cretaceous. SEM observations and Raman analysis suggest that the skin of Gansus yumenensis may be pigmented. Elemental mapping indicates that aluminosilicates and calcium phosphate are involved in the mineralization of the skin.
The photon beamline vacuum system of the European XFEL
Authors: Dommach Martin , Di Felice Massimiliano , Dickert Bianca , Finze Denis , Eidam Janni , Kohlstrunk Nicole , Neumann Maik , Meyn Frederik , Petrich Michaela , Rio Benoit , Sinn Harald , Villanueva Raúl 
Published: June 2021

The photon beamline vacuum system of the European X-ray Free-Electron Laser Facility (European XFEL) is described. The ultra-large, in total more than 3 km-long, fan-like vacuum system, consisting of three photon beamlines is an essential part of the photon beam transport. It is located between the accelerator vacuum system and the scientific instruments. The main focus of the design was on the efficiency, reliability and robustness of the entire system to ensure the retention of beam properties and the operation of the X-ray optics and X-ray photon diagnostics components. Installation started in late 2014, the first of the three beamline vacuum systems was commissioned in spring 2017, and the last one was operational in mid-2018. The present state and experience from the first years of operation are outlined.
Visualization concept for maintenance information using wearable devices
Authors: Dannenmann Peter 
Published: June 2021

Purpose
The purpose of this paper is to show the current state of methods for information visualization on wearable devices and demonstrate how these devices and methods can be used to support the work of aircraft maintenance engineers. This paper develops a path to make aircraft maintenance tasks more efficient and safe by providing relevant and important information to maintenance personnel matching the task they are currently working on.

Design/methodology/approach
The current researchers elaborate prerequisites that wearable computing devices have to meet to use them in aircraft maintenance. Based on these prerequisites the researchers evaluate such existing devices and visualization techniques with regard to how they can be used to visualize information in aircraft maintenance processes.

Findings
This paper elaborates a roadmap how wearable devices can be applied in future aircraft maintenance scenarios. It also shows that augmented reality (AR) is a technology that shows great potential to support aircraft maintenance staff. The current researchers develop a scenario, how AR applications on wearable devices support such tasks.

Practical implications
The implementation of the maintenance scenario that is developed in this paper will vastly reduce the possibilities of mistakes by maintenance personnel. This leads to more efficient maintenance processes and further increases the safety of aviation in general.

Originality/value
Although AR today already is used in fields of application like gaming, an application of this technology in a safety-critical environment is not known to the author. This paper provides a step towards extending the application domains of this technology to aircraft maintenance.
Extending UTAUT2 in M-banking adoption and actual use behavior: Does WOM communication matter?
Authors: Farzin Milad , Sadeghi Marzieh , Yahyayi Kharkeshi Fatemeh , Ruholahpur Hedyeh , Fattahi Majid 
Published: June 2021

Purpose
The purpose of this study is to investigate important factors that help explain customer willingness to adopt mobile banking (M-banking). To this end, the unified theory of acceptance and use of technology 2 (UTAUT2) was applied and to more accurately predict customer behavioral intentions, it was attempted to extend it.

Design/methodology/approach
The research data were collected from 396 customers of Iranian private banks who had the experience of using M-banking. The structural equation modeling technique was used to test the research hypotheses.

Findings
Findings suggest that performance expectancy, effort expectancy, social influence, facilitating conditions, habit, hedonic motivation, perceived value and trialability are endorsed as proponents of M-banking adoption intention. On the other hand, M-banking adoption intention has also had a significant positive effect on actual use behavior and word-of-mouth (WOM). WOM has also influenced actual use behavior and mediated the relationship between M-banking adoption intention and actual use behavior.

Research limitations/implications
The present study focuses on private banks, therefore, although it is sufficient, it is limited to private cases. This study contributes to the literature on M-banking services and actual use behavior. By appropriately focusing on M-banking adoption intention and the service quality provided, banks can strengthen their relationships with customers, thereby stimulating actual customer behavior such as actual use behavior and WOM.

Originality/value
From theoretical and managerial aspects, this study has particular value for the literature on M-services’ intention in general and banking in particular. The present study provides a conceptual framework for M-banking adoption intention, which could be used in M-banking services. In addition, this study sought to extend UTAUT2 and to examine the mediating role of WOM in actual use behavior motivation as well.
Is the use of financial accounting fit for purpose? An exploration of the theoretical foundations, framework and practicalities
Authors: de Aguiar Thereza Raquel Sales 
Published: June 2021

Purpose
The purpose of this paper is to explore issues related to the use of financial accounting and reporting by discussing three interrelated areas: the theoretical foundations, the framework and practicalities. The paper also discusses participatory and pluralistic approaches to accounting and corporate governance as alternatives to address some of these issues.

Design/methodology/approach
This is a narrative research based on deductive thematic analysis of secondary data. This study provides a general overview of the existing literature of the limits of the use of financial accounting and its impact on business and society.

Findings
In terms of the theoretical foundations, this paper contrasts financial accounting explained by agency theory and a dialogic accounting approach. The findings of this study emphasise the need to establish an accounting framework for the interests of the many (not the few) in conjunction and simultaneously with a participatory and pluralistic approach to corporate governance. Finally, this paper explores accounting for carbon emissions and recent financial accounting scandals to analyse the impact of the inappropriate use of financial accounting and reporting in business and society.

Originality/value
This paper provides an overview of the limits of the use of financial accounting by exploring its theoretical background, framework and practicalities. The paper also discusses the need for new accounting and corporate governance frameworks that allow a pluralistic and participatory approach to the decision-making of companies.
Floral diversity in the central part of Chattogram city, Bangladesh
Authors: Biswas Trapa , Dutta Shourav , Hossain Md. Akhter , Rahman Md. Rayhanur , Hossen Saddam , Hossain Mohammed Kamal 
Published: June 2021

Purpose
This study/paper aims to evaluate the floral richness of the central part of Chattogram city, Bangladesh. Chattogram is recognized as the largest port city and the commercial capital of Bangladesh, which confronts faster urbanization and swift infrastructure development. Green spaces in and around Chattogram city are shrinking sharply, which resulted in rapid loss of floral and faunal resources in this area. The present study was carried out from February 2018 to January 2019 to enumerate the vascular plant species of the Sulakbahar ward located in the central part of Chattogram City, Bangladesh.

Design/methodology/approach
The study area was categorized into 10 habitats to assess the variation of floral composition. The extensive whole area survey method was applied to record the flora from all sorts of plant habitats of the research area.

Findings
The study enumerated 418 vascular plant species under 315 genera and 120 families including natural, planted and cultivated from the study area. The habit form of the recorded plant composition indicated that herbs (35%) constitute the major plant category followed by trees (34%), shrubs (17%), climbers (12%), ferns (1%) and orchids (1%). The study also indicated that exotic species (50.3%) became dominant than native species (49.7%) in Chattogram city because of their scenic beauty, easy propagation and ornamental value to the city planners and inhabitants.

Originality/value
It appeared that floral resources of the Chattogram city area are in great threat due to aggressive and unplanned infrastructure development for housing, offices and institutions by replacing the green spaces. The study recommended that urgent protection measures should be taken to conserve and protect the existing floral resources for the well-being of the urban people.
The effect of multilingual suggested tags on cross-language information tagging behaviour
Authors: Ma Xiaoyue , Zhang Siya , Zhao Pengwei 
Published: June 2021

Purpose
Suggested tag was considered as one of the critical factors affecting a user’s tagging behaviour. However, compared to the findings on the suggested tags for the monolingual environment, it still lacks focused studies on the tag suggestions for cross-language information. Therefore, this paper aims to concern with annotation behaviour and psychological cognition in the cross-language environment when suggested tags are provided.

Design/methodology/approach
A cross-language tagging experiment was conducted to explore the impact of suggested tags on the tagging results and process. The descriptive statistics of tags, the sources and semantic relations of tags, as well as the user’s psychological cognition were all measured in the test.

Findings
The experimental results demonstrated that the multilingual suggested tags could bring some costs to a user’s tagging perception. Furthermore, the language factor of suggested tags led to different paths of tagging imitation (reflected by longer semantic mapping and imitation at the visual level) and different cognitive processes (topic extraction and inference process).

Originality/value
To the best of the authors’ knowledge, this study is one of the first to emphasize the effect of suggested tags during multilingual tagging. The findings will enrich the theories of user-information interaction in the cross-language environment and, in turn, provide practical implications for tag-based information system design.
Numerical investigation of double-diffusive mixed convection of Fe3O4/Cu/Al2O3-water nanofluid flow through a backward-facing-step channel subjected to magnetic field
Authors: Nath Ratnadeep , Murugesan Krishnan 
Published: June 2021

Purpose
This study aims to investigate the buoyancy-induced heat and mass transfer phenomena in a backward-facing-step (BFS) channel subjected to applied magnetic field using different types of nanofluid.

Design/methodology/approach
Conservation equations of mass, momentum, energy and concentration are used through velocity-vorticity form of Navier–Stokes equations and solved using Galerkin’s weighted residual finite element method. The density variation is handled by Boussinesq approximation caused by thermo-solutal buoyancy forces evolved at the channel bottom wall having high heat and concentration. Simulations were carried out for the variation of Hartmann number (0 to 100), buoyancy ratio (−10 to +10), three types of water-based nanofluid i.e. Fe3O4, Cu, Al2O3 at χ = 6%, Re = 200 and Ri = 0.1.

Findings
The mutual interaction of magnetic force, inertial force and nature of thermal-solutal buoyancy forces play a significant role in the heat and mass transport phenomena. Results show that the size of the recirculation zone increases at N = 1 for aiding thermo-solutal buoyancy force, whereas the applied magnetic field dampened the fluid-convection process. With an increase in buoyancy ratio, Al2O3 nanoparticle shows a maximum 54% and 67% increase in convective heat and mass transfer, respectively at Ha = 20 followed by Fe3O4 and Cu. However, with increase in Ha the Nuavg and Shavg diminish by maximum 62.33% and 74.56%, respectively, for Fe3O4 nanoparticles at N = 5 followed by Al2O3 and Cu.

Originality/value
This research study numerically examines the sensitivity of Fe3O4, Cu and Al2O3 nanoparticles in a magnetic field for buoyancy-induced mixed convective heat and mass transfer phenomena in a BFS channel, which was not analyzed earlier.
AI-enabled knowledge sharing and learning: redesigning roles and processes
Authors: Sundaresan Shankar , Zhang Zuopeng 
Published: June 2021

Purpose
This paper aims to investigate the role of AI in facilitating knowledge sharing and learning in organizations and the redesign of AI-enabled knowledge workers’ roles and processes.

Design/methodology/approach
This paper develops a framework for analyzing AI’s role in different knowledge management activities, explores the impact of AI in transforming knowledge workers’ roles and processes in knowledge sharing and learning and presents recommendations for tailored AI-enabled knowledge management systems for modern knowledge worker environments.

Findings
The authors synthesize the elements from different parts of the relevant literature and develop a unified framework consisting of three dimensions of AI systems, three knowledge management (KM) activities and two types of AI–human interactions. Based on this framework, the authors summarize the primary use cases supported by AI-enabled knowledge management systems (KMS) and compare them with the traditional KMS use cases. The authors find that a single type of AI system is insufficient to support the increasingly complex nature of knowledge workers’ activities, manifested in three dimensions – process, engagement and content; a tailored AI system should be developed to support knowledge workers in their unique roles and processes.

Originality/value
With the growing interest in AI and its applications to KM, this research provides managerial insights for practitioners to effectively adopt AI in managing knowledge assets in organizations.
Orchestrating frugal eco-innovation: the plethora of challenges and diagnostics in lean startups of emerging economies
Authors: Abbas Syed Mudasser , Liu Zhiqiang 
Published: June 2021

Purpose
Sustainable development research assumes that startups, under extreme financial constraints, cannot sacrifice resources now for benefits later without risking their survival. Furthermore, their non-compliance with environmental regulations adds fuel to the fire. This paper aims to explore the challenges faced by startups in resource-scarce economies and the innovative ways of coping with these challenges.

Design/methodology/approach
The data for the study was collected through 17 semi-structured interviews taken from startup owners and industry experts based in Pakistan and Bangladesh. The transcribed data were coded through NVivo 12 and themes were generated by merging 47 open and 14 axial codes.

Findings
The findings show that a lack of government support and lack of organisational readiness and motivation significantly affect startups’ frugal eco-innovation. Empirical evidence reveals problems related to the business ecosystem, and internal organisational issues also contribute to challenges faced by startups in attaining a competitive position in the industry.

Research limitations/implications
The study’s findings suggested leveraging dynamic capabilities can help lean startups in frugal eco-innovation. Furthermore, organisational cohesion, business ecosystem, government regulations and assistantship, organisational mismanagement and market realisation are decisive in startups’ competitive position in emerging economies.

Practical implications
The findings of the study will result in a higher adoption rate of more competitive business models, and hence, startups’ sustainability. The results would be an effective and efficient deployment of sustainable technological solutions, creating more customer and shareholder value leading to economic growth.

Originality/value
This research offers a comprehensive analysis of frugal eco-innovative startups by exploring the interplay between different challenges and organisational capabilities. Furthermore, the study contributes to the existing body of knowledge by providing empirical evidence that eco-innovation can be conducted in a resource-constrained environment. This study challenged the scholarly and managerial assumption of the availability of finances as a significant player in eco-innovation. The study also links the Darwin theory of startups to a competitive edge over rivals for startups’ survival.