bluuebunny/crossref_metadata_2025_split huggingface dataset:
Rno-miR-199a-3p targets Nedd4 to promote sensitization of ST36 acupoints via mast cell activation in a rat model of knee osteoarthritis
Authors: Qi Wenchuan , Liu Baitong , Jiang Yilu , Luo Xinye , Li Zhiwei , Zheng Qianhua , Liang Fanrong , CAI Ding-jun 
Published: October 2021

Abstract
Selecting routine points on related meridians is widely accepted as the foundational principle of acupuncture. When the body is suffering disease or injury, corresponding acupoints are thought to be activated and manifest in several sensitized forms. Sensitized acupoints hold high clinical value as a reflection of disease activity on the body surface. Mast cells have been implicated in the process of acupoint sensitization but the underlying regulatory mechanisms remain unclear. In the present study, we evaluated ST36 as a sensitized acupoint in the monosodium iodoacetate-induced knee osteoarthritis rat model. We first confirmed sensitization at the ST36 acupoint through decreases in the acupoint mechanical pain threshold and instructively found an accompanying increase in skin mast cell degranulation. Thereafter, we used highthroughput RNA sequencing to reveal potential molecular mechanisms of acupoint sensitization. We showed that rno-miR-199a-3p was highly expressed in the sensitized ST36 acupoint and its expression was associated with mast cells. Functional experiments revealed that overexpression of rno-miR-199a-3p increased mast cell histamine release whereas inhibition of rno-miR-199a-3p decreased histamine release. Mechanistically, we established rno-miR-199a-3p acted to inhibit neural precursor cell expressed developmentally down-regulated 4 (Nedd4) protein expression through miRNA-mediated targeting of the 3’-UTR of Nedd4 mRNA. Moreover, we found ectopic expression of Nedd4 antagonized histamine release in mast cells and blocked the actions of rno-miR-199a-3p overexpression. Thus, our study establishes that mast cells participate in the process of acupoint sensitization, and further reveals a novel miRNA-based mechanism which is crucial for further understanding of acupoint sensitization and acupuncture applications.
BUB1 Predicts Poor Prognosis and Immune Status in Liver Hepatocellular Carcinoma
Authors: qi wenbo , Bai Yuping , Liu Le , Chen Hao 
Published: October 2021

Abstract
Background: Accurate assessment of the tumour immune microenvironment helps develop individualised immunotherapy regimens and screen dominant populations suitable for immunotherapy. Therefore, potential molecular markers were investigated to make an overall assessment of the immune microenvironment status of liver hepatocellular carcinoma (LIHC). Methods: Differentially expressed genes (DEGs) in LIHC were extracted from the International Cancer Genome Consortium and The Cancer Genome Atlas databases. Gene set enrichment analysis was employed to assess the function of DEGs. Hub genes were identified using the STRING tool. The prognostic value of the hub genes was evaluated through Kaplan–Meier analysis and Cox regression. The correlation between genes and immunity was analysed using the TIMER tool. Further, tissue samples from 42 LIHC patients were collected for immunohistochemistry. HuH7 and SKP1 cells were analysed via western blotting, Cell Counting kit-8 assay, and Transwell assay. Results: A total of 121 DEGs were identified, and DEGs were enriched in the epithelial-mesenchymal transition, hypoxia, myogenesis, and p53 pathways. A total of 20 hub genes were selected and a strong correlation was identified between these hub genes and prognosis. The expression of budding uninhibited by benzimidazoles 1 (BUB1), which is known to play a role in cancer progression, was found to be upregulated in LIHC (compared to normal tissues). Furthermore, BUB1 expression was strongly related to immune cells and immune checkpoint molecule expression. Immunohistochemistry indicated that BUB1 expression was higher in LIHC tissues than in normal liver tissues. Western blotting showed that BUB1 expression was the highest in HuH7 and SKP1 cells. BUB1 knockdown resulted in reduced proliferation and vertical migration ability of LIHC cells, and reduced the expression of phospho-SMAD family member 2 and phospho-SMAD family member 3 proteins. Immunohistochemistry showed that BUB1 expression was accompanied by immune cell infiltration into LIHC tissues.Conclusions: These results suggest that BUB1 may serve as a potential prognostic biomarker for LIHC and as an indicator of its immune status.
Flexible ureteroscope with ultrasound guidance for treatment of parapelvic renal cysts: A complementary approach to locate the cystic wall
Authors: Yan Kun-Wu , Tian XIao-Fei , Meng Na , Liu Wen-Zhan , Lu Zhi-Min , Guo Ming-Tao , Xiao Bo 
Published: October 2021

Abstract
Background

The main treatment of parapelvic cysts is flexible ureteroscope currently. Considering the intraoperative localization of the cyst may fail with flexible ureteroscope, we tend to use an innovative method by ultrasound-guided for easily locating cystic wall during flexible ureteroscopic surgery
Methods

We retrospectively reviewed 17 consecutive cases of parapelvic renal cysts treated by ultrasound-guided flexible ureteroscope between March 2017 and May 2020. The differences of simple flexible ureteroscopic technique and ultrasound-guided flexible ureteroscopic technique were compared. The surgical procedures, postoperative complications, results and patients’ follow-ups were evaluated.
Results

The cysts wall were seen clearly in 10 patients with ureteroscopic vision. Another 7 patients changed to ultrasound-guided flexible ureteroscopic surgery since it was difficult to identify the cyst wall. Mean operative time were 25.9 ± 8.7 minutes and 37.1 ± 10.1 minutes for conventional and modified technique respectively (P =0.004), of which 17.6 ± 5.8 minutes and 26.5 ± 8.4 minutes to search the cysts, respectively (P = 0.002), and the mean time of the incising were 7.1 ± 4.9 minutes and 12.1 ± 5.6 minutes, respectively (P = 0.000). All of the patients were followed-up 12 months, there were no serious complications and recurrence observed.
Conclusions

We demonstrated that it is feasible and safe to treat parapelvic renal cyst by ultrasound-guided flexible ureteroscopic incision and drainage. The less sample size and further studies were the limitations of our study.
Separation of butane isomers using a novel ZIF-8 slurry with extremely high efficiency: from laboratory study to industrial pilot test
Authors: Yang Mingke , Wang Huishan , Zuo Julian Y. , Han Yu , Chai Liya , Li Kun , Chen Wan , Peng Xiaowan , Dong Baocan , Deng Chun , Sun Chang-Yu , Liu Bei , Chen Guang-Jin 
Published: October 2021

Abstract
Both n-butane and iso-butane are important petrochemical raw materials, and their separation is of great significance and full of challenge because of their close boiling characteristic. The ZIF-8 slurry formed with a novel mixed solvent M-water solution as sorption media was adopted to separate the mixture with high efficiency. The iso-/n-butane selectivity of ZIF-8/M-water slurries is as high as 890 with high kinetic performance, even transcending the upper limit of the new materials or membranes. More encouragingly, continuous pilot separation device was established and test results show that the purity and recovery ratio of iso-butane product achieved 99.46 mol% and 87% respectively, which are remarkably superior to the corresponding performance (98.56 mol% and 54%) of the industrial distillation tower. To our best knowledge, this is the first industrial pilot test using MOFs for gas separation, and also an important step forward to the commercial application of MOFs in gas separation.
Effects of Socio-economic Factors on Research Over Systemic Sclerosis: an Analysis Based on Long Time Series of Bibliometric Data
Authors: Guo Wei , Zhou Zeyu , Liang Yinhe , Xu Chuanhui , Zeng Lin , Dong Zhiyong , Mu Rong 
Published: October 2021

Abstract
Background Systemic sclerosis (SSc) is a rare detrimental disease warranting mobilization of global research efforts. Evaluating how socio-economic factors impact country research output on SSc could help to identify solutions advancing SSc research.Methods Publication production on SSc during 1969–2018 and data for structural and policy factors for WHO member countries were collected from public sources. Associations between SSc research output and country-level factors were investigated through panel regression. Difference-in-differences analysis further assessed the causal effects of rare disease legislation. Results SSc publications showed an increasing annual growth rate (−0.3% during 1969–1983 vs. 6.9% during 2000–2018), but were concentrated in high-income countries (HICs). Ten countries, nine of which were HICs, published 12 261 (77.5%) SSc publications but another 87 countries produced none. GDP, population and expenditure on research and development were positively associated with SSc publications (p<0.001). Higher health expenditure was only found to be associated with increased SSc publications in HICs (p<0.001). Rare disease legislation increased annual publication production by 62.8% (95% CI 0.390–0.867; p<0.001) averagely. In MICs, the effect was especially swift and lasting. No significant impact was found with GDP per capita, female percentage, and political indicators.Conclusions SSc research output increased over time with substantial country disparities. Effective health policies facilitating research should be expanded especially among MICs to accelerate research advancement.
Effect of Knowledge Translation on Suspected Venous Thromboembolism in Hospitalized Patients
Authors: Peng Fei , Zhang Quan , Zeng Xiaohui , Fu Min , Li Jiang , Tan Xiaoju , Li Yifan , Qiu Jian , Zhu Yazhi , Li Meizhi , Zhong Yanjun , Wu Shangjie , Zhang Yan 
Published: October 2021

Abstract
Background Venous thromboembolism (VTE) is a preventable disease, but the implementing effect of preventive measures is not optimistic, and few patients receive optimal prophylaxis, which result in significant mortality, long-term complications and financial burden. The purpose of the article was to explore the effect of knowledge translation on suspected venous thromboembolism in hospitalized patients on admission.Methods The data of suspected VTE patients on admission was retrospectively collected from the Second Xiangya Hospital from October 2016 to September 2020. The Mann-Whitney test and Fisher’s exact test (or χ 2 test) were used to analyze the continuous and categorical variables. A decision tree model was developed to assess the cost-effectiveness of post-prophylaxis education compared with pre-prophylaxis education. The ranges of each parameter were set to perform sensitivity analysis.Results The awareness of preventable VTE has raised which expressed in the increasing use of Caprini score, incidence of VTE, and department distribution of suspected VTE patients on admission. The incremental cost effectiveness ratio was a negative value which was significantly smaller than willing to pay (WTP). When the WTP value was ¥212676, the probability of patients' acceptance was 84.3% in the post-prophylaxis group and 15.7% in the pre-prophylaxis group. No matter how WTP changed, the cost-effectiveness acceptability of post-prophylaxis education in preventing VTE after hospitalization was better than pre-prophylaxis education did. Conclusion Knowledge translation on VTE could have cost effectiveness on suspected VTE in hospitalized patients on admission.
A Risk Assessment Model of Immune-Associated lncRNA to Predict the Prognosis and Immune Landscape of Renal Clear Cell Carcinoma
Authors: Ma Tiantian , Zhu Cuiwen , Duan Yiping , Chen Lingyue , Liu Jiacui , Li Dongxu , Yu Mingxia , Yang Gui 
Published: October 2021

Abstract
Renal cell carcinoma (RCC) is one of the most common malignancies of the urinary system, accounting for 3% of adult malignancies. Long non-coding RNA (lncRNA) is abnormally regulated in many cancers and can be used as a molecular marker for early diagnosis and prognosis of RCC. Here, original lncRNA datas were retrieved from TCGA, differential co-expression analysis was performed to classify immune-related lncRNA (irlncRNA) with differential expression, and the improved 0 or 1 matrix cyclic single pairing method was used to verify lncRNA pairs. Then, we performed a univariate analysis in combination with an improved Lasso penalty regression that included cross-validation, multiple repetitions, and random stimulus procedures to determine different expression irlncRNA (DEirlncRNA) pairs. AUC values under Receiver Operating Characteristic curve (ROC) were calculated to obtain the optimal model, and AIC values of each point on AUC were calculated to obtain the optimal cut-off point to distinguish the high and low risk groups of Clear-cell renal cell carcinoma (ccRCC) patients. Finally, we evaluated the new model in a variety of clinical settings including survival, clinicopathological features, tumor-infiltrating immune cells, chemotherapy, and checkpoint related biomarkers, all showing promising clinical application.
The Magnitude of Pre-lacteal Feeding Practice and its Influencing Factors Among Mothers with Children Under the Age of Two Years in Afar Region of Ethiopia: A Community based Quantitative and Qualitative Study
Authors: Gebeyehu Temesgen , Gebru Abel , Agegnehu Nigus 
Published: October 2021

Abstract
Background: Pre-lacteal feeding is a barrier to implement optimal breastfeeding practices and caused new-born disease. Pre-lacteal feeding is primarily practiced in developing countries, where cultural and social structures are based on descent and relationships. The aim of study was to determine the magnitude of pre-lacteal feeding practice and its influencing factors among mothers with children under the age of 2-years in Afar region of Ethiopia. Method: A community based cross sectional study supplemented by qualitative study was employed. In the quantitative study, 235 study participants were included, whereas seventeen study subjects participated in the qualitative study. Bivariate and multivariate logistic regression was used to determine factors associated with pre-lacteal feeding practice. The qualitative data was analyzed using thematic approach. Result: The magnitude of pre-lacteal feeding was 36.6%. Being a housewife (AOR=4.1, 95% CI: 1.4-12.2) and having male indexed child (AOR=4.9, 95% CI: 1.8-13.5) were more likely to practice pre-lacteal feeding than those of trade and female, respectively. However, pre-lacteal feeding practice decreased in those mothers with three and above ante natal care visits (AOR=0.31, 95% CI: 0.1-0.9), delivered at health institutes (AOR=0.1, 95% CI: 0.02-0.3), starting breastfeeding within one hour (AOR=0.04, at 95% CI: 0.01-0.1) and getting breast feeding counseling (AOR=0.21, at 95%CI: 0.06-0.7). Milk, plain water, sugar solution, honey, butter, dates (‘temir'), a leaf, and condo pepper were the most common pre-lacteal foods reported by study participants. Cultural beliefs and the assumption of cleaning new-born throat and bowel were the most common reason to practice pre-lacteal feeding. Conclusion: The magnitude of pre-lacteal feeding was high due to socio-cultural and maternal health service utilization factors. Water, milk products, sweet foods and some of plant types were the most common pre-lacteal foods. Key words: Pre-lacteal feeding, factors, mother, children
Endophenotype-Wide Association Study Reveals Genetic Substrates of Core Symptom Domains and Neurocognitive Function in Autism.
Authors: Lee In-Hee , Koelliker Ekaterina , Kong Sek Won 
Published: October 2021

Abstract
Background Autism is a neurodevelopmental disorder largely attributable to rare and common genetic variants. Additionally, environmental factors such as maternal immune activation and air pollution exposure can also increase the risk of autism. Genetic heterogeneity of autism has been well-recognized from gene discovery efforts over the past decade; however, genetic substrates of endophenotypes that constitute phenotypic heterogeneity are not known yet. Methods Whole-genome sequencing (WGS) data and a set of phenotype scores that represent neurocognitive development and the severity of core symptoms of autism were collected from the iHART and MSSNG databases and the phenotype database of Autism Speaks. Endophenotype-wide association analysis was performed with genome-wide genotype and 29 phenotype scores. Results One or more genetic loci were associated with each of phenotype scores at a genome-wide significance threshold ( P =5×10 -8 ) except for a total score of the Social Responsiveness Scale-2. An intergenic locus on chromosome 15q26.1 was significant for three core symptom domain scores of ADOS Module 1 while each phenotype score was associated with a unique set of genetic loci. The Repetitive Behaviors Scale total score was associated with the largest number of loci (N=132) including the loci that overlapped with the genes involved in brain development and neurodegenerative disorders. Among the significant genotype-endophenotype associations, verbal intelligence and the OSTN gene was notable. The secretory peptide osteocrin—encoded by OSTN —is implicated in activity dependent dendritic growth in human and has potential for a biomarker of autism and an endophenotype marker for verbal intelligence. Limitations Validation of our findings in another cohort is required. Several associations involving the ADI-R and ADOS scores may indicate inherited allelic differences between affected and unaffected individuals since unaffected siblings were included in our analysis. Conclusions Our results suggest that autism candidate genes discovered by case-control GWAS may include trait-associated genes for core symptoms.
Comprehensive Characterization of Transgene Integration in a Similar Intragenesis Rice Event Using Paired-end Whole-genome Sequencing Approach
Authors: Xu Wenting , Zhang Hanwen , Zhang Yuchen , Li Rong , Yang Litao 
Published: October 2021

Abstract
Efficient, accurate molecular characterization of genetically modified (GM) organisms is challenging, especially for novel transgenic products of cisgenesis/intragenesis transferred with genes/elements of recipient species. Herein, GM rice event G281, involving transfer with native promoters and an RNA interference (RNAi) expression cassette in a process similar to intragenesis, was subjected to molecular characterization using paired-end whole genome sequencing (PE-WGS). The results showed that transgenes integrated at rice chromosome 3 locus 16,439,674 included a 36 bp deletion of rice genomic DNA, and the whole integration contained two copies of the complete transfer DNA (T-DNA) in a head-to-head arrangement. No unintended insertion or backbone sequence of the transformed plasmid were observed at the whole genome level. Molecular characterization of the G281 event will assist risk assessment and application for a commercial license. Additionally, the findings demonstrate the applicability of PE-WGS for molecular characterization of cisgenesis/intragenesis crops.
Study on the Effect of Porosity on Countercurrent Hollow Fiber Membrane Humidification System
Authors: NIU Runping , Jia Xiaoting , Geng Lizhi 
Published: October 2021

Abstract
The effect of porosity on the humidification efficiency of countercurrent hollow fiber membrane humidification system was investigated by using numerical simulation method to study polypropylene (PP) porous fiber membrane material. Firstly, the correctness of the numerical model was verified by experiments, and then the influence of porous fiber membrane material on humidification efficiency was further explored by changing the porosity of the model. The simulation results show that the humidification capacity and efficiency of the humidification component increase with the increase of porosity. When the porosity is between 0.35-0.8, the humidification capacity and efficiency increase significantly. However, when the porosity is between 0.8-0.9, although the humidification amount and humidification efficiency value are high, the increment is not obvious, and the porosity of the fiber film is inversely proportional to the support strength of the film, and the larger the porosity is, the shorter the service life of the film material is. Therefore, it is suggested to design the porosity of polypropylene (PP) film material between 0.65 and 0.8. It can not only ensure the high humidification capacity and efficiency of the fiber membrane, but also prolong the service life of the membrane.
Cerebellar Tonsillar Herniation in Sudden Death of An Adolescent Anorexia Nervosa Patient: A Case Report.
Authors: Kitajima Tasuku , Otani Ryoko , Matsushima Naho , Matsubara Naoki , Inoue Takeshi , Fujii Akiko , Ban Shinichi , Sakuta Ryoichi 
Published: October 2021

Abstract
Background: Anorexia nervosa not only results in severe malnutrition but also carries a high risk of sudden death. Although fatal arrhythmias are the most common cause of sudden death, it is often unclear what exactly causes them. To the best of our knowledge, there have been no reports of cerebral herniations in patients with anorexia nervosa.Case presentation: A 17-year-old adolescent girl with neuropathy and autism spectrum disorder was admitted to the pediatric ward for extreme weight loss. Eight hours after admission, she suddenly went into cardiopulmonary arrest and died despite resuscitation. A postmortem autopsy revealed the unexpected findings of generalized severe cerebral edema and cerebellar tonsillar herniation.Conclusion: Intracranial hypertension may need to be considered when the condition of patients with anorexia nervosa suddenly worsens during refeeding periods. Postmortem autopsy and autopsy imaging are recommended to determine the exact cause of sudden death.