bluuebunny/crossref_metadata_2025_split huggingface dataset:
ANALISIS PENGALAMAN PENGGUNA PADA WEBSITE PROGRAM STUDI DESAIN KOMUNIKASI VISUAL UNIVERSITAS DIAN NUSWANTORO
Authors: Ihya'ulumuddin Dimas Irawan , Sulistiyawati Puri 
Published: March 2021

Website merupakan media yang kini banyak digunakan untuk mendapatkan informasi melalui jaringan internet. Sebagai program studi dibidang seni dan desain, DKV Udinus (Desain Komunikasi Visual Universitas Dian Nuswantoro) menggunakan website sebagai media pendukung untuk menyampaikan informasi kepada mahasiswa mengenai profil jurusan, kurikulum, portofolio karya, kegiatan mahasiswa seperti pameran, sampai dengan alumni. Namun, selama ini belum pernah dilakukan evaluasi sehingga tidak dapat dilakukan pengukuran apakah website DKV sudah memenuhi aspek usability bagi pengguna. Oleh sebab itu dalam penelitian ini dilakukan evaluasi website DKV UdinusÂ untuk mengukur kemudahan pengguna. Adapun proses evaluasi dalam penelitian ini menggunakan usability testing. Tahapan penelitian diawali dengan penyebaran kuesioner kepada mahasiswa dan dosen DKV Udinus sebagai responden. Hasil penelitian menunjukkan penilaian dari 5 variable yang diujikan, diperoleh kesimpulan bahwa aspek learnbility dan memorability pada website DKV Udinus memiliki penilaian yang cukup bagus. Namun penilaian 3 aspek lainnya masih rendah, sehingga masih diperlukan perbaikan berkaitan dengan aspek efficiency, error dan satisfaction untuk mempermudah penggunaan dalam mengakses informasi pada website DKV Udinus.
Kajian pengelolaan sampah rumah tangga (SRT) dan sampah sejenis sampah rumah tangga (SSRT)) di Kabupaten Teluk Bintuni
Authors: Riruma Nicolas , Sinaga Nurhaida , Lekitoo Marlyn N. 
Published: March 2021

The objectives to be achieved from this research are to determine the amount of waste generation and characteristics, evaluate waste management in 2019, project a waste balance for 2020-2025, and identify the form of waste management policies, strategies and programs for 2020-2025 in Teluk Bintuni Regency. The results of the study, the amount of waste generation was 3.46 l / o / h; composition of organic waste 67.12%, plastic waste 26.30%, and other inorganic waste 6.75%; managed waste 65.55%; served population 76.90%; implementation of operational technical standards 45.43%; effectiveness of the role of local government 39.49% (less effective criteria); low community behavior in waste management (69%), community perception in good waste management (81%); projected waste balance for 2020-2025 waste generation 169,229.79 tons, 30% reduction target (44,427 tonnes), 70% handling target (122,596 tonnes), 100% managed waste by 2025 (169,229.79 tonnes); 2 forms of policies, 6 strategies and 17 waste management programs were identified for 2020-2025.
Preclinical Study of the Efficacy and Safety of Chondroitin Sulfate
Authors: Mazukina E. V. , Shekunova E. V. , Kosman V. M. , Urakova I. N. , Kotelnikova I. G. , Fonarev M. Yu. , Ezhova E. A. , Zakalyukina E. V. , Makarova M. N. , Makarov V. G. 
Published: March 2021

Chondroitin sulfate is used for osteoarthritis combination therapy. It should be taken into account that the structure and properties of polysaccharides included in chondroitin sulfate, as well as the raw materials used for its production, have a significant effect on its absorption, bioavailability, and, as a consequence, on the safety and efficacy of orally administered products.The aim of the study was to assess toxic properties, local irritant effect, immunotoxicity, basic pharmacokinetic parameters, and therapeutic efficacy of the new Chondroitin sulfate product (produced by Federal State Unitary Enterprise “Moscow Endocrine Plant”, Russia) as compared to Structum (produced by “Pierre Fabre Medicament Production”, France).Materials and methods: White Giant rabbits were used in the experiments. Toxicity, immunotoxicity and local irritation effects of the products were assessed following daily oral administration at the dose of 168 mg/kg (about 6 Maximum Recommended Therapeutic Doses) to male and female rabbits for 28 days. The follow-up period was 14 days. The pharmacokinetic study included blood sampling on days 1‒2 of the experiment, complete blood count and blood chemistry tests were performed on days 28 and 43. After killing the animals, pathomorphological and histological examinations were performed on their organs and tissues. Therapeutic efficacy was studied in an osteoarthritis model made by cruciate ligament transaction in rabbits. The animals received therapy at doses of 16.8 mg/kg, 33.6 mg/kg, and 67.2 mg/kg for 56 days starting from day 8 after the pathology induction.Results: the medicines had no toxic, local irritant, or immunotoxic effect. The NOAEL was established at 168 mg/kg. The study demonstrated the comparability of the pharmacokinetic profiles of the studied products following single oral administration. The maximum concentration of the active ingredient (Cmax = 79 ± 6 μg/mL—Chondroitin sulfate; Cmax = 71 ± 4 μg/mL— Structum) in blood plasma was observed within 3–4 hours after administration. A decrease in the severity of cartilage structural damage was observed for the doses of 33.6 mg/kg and 67.2 mg/kg. The results of quantitative determination of sulfated glycosaminoglicans in the proteoglycans of the cartilage articular surface in the animals with osteoarthritis demonstrated an increase in the level of sulfated glycosaminoglicans in the groups treated with the maximum doses of the studied products, as compared to the other groups.Conclusions: the obtained data confirm that the test product has a favourable safety profile, and therapeutic (chondroprotective) effect. All the tested properties of Chondroitin sulfate were comparable to those of Structum.
Digestional diseases in Covid‑19 in children
Authors: Kharitonova L. A. , Osmanov I. M. , Ploskireva A. A. , Solodovnikova O. N. , Milova Yu. E. 
Published: March 2021

The new coronavirus infection (COVID‑19) caused by the SARS-CoV‑2 coronavirus is rightfully recognized as a pandemic of the 21st century and poses a threat to the health of all human race. According to the World Health Organization, at October 4, 2020, were registered worldwide 34,804,348 confirmed cases of COVID‑19, including 1,030,738 deaths. According to currently available data, children accounted for 1 to 5% of patients diagnosed with COVID‑19 [4]. Children aged between 10–18 years dominated (45.9%), and patients first years of life accounted 26.1%. At the same time, over the period since the beginning of the pandemic, only a few fatal outcomes of the disease in children have been recorded in world statistics.The high contagiousness of the SARS-CoV‑2 coronavirus, the ability for long-term replication, and tropism for the cells of the immune system provide the virus with the possibility of almost simultaneous multiple organ damage with the rapid development of complications of both the respiratory tract and other organs and systems.The gastrointestinal tract is involved in this pathological process, representing one of the “shock organs”. In addition to common respiratory symptoms, some COVID‑19 patients experience dyspeptic disorders such as diarrhea, nausea, and vomiting. Anal swab samples from patients positive for COVID‑19, are positive for SARS-CoV‑2 nucleic acid, and SARS-CoV‑2 were isolated from stool samples from COVID‑19 patients. In addition, in both adults and children, there is a strong association between damage to the digestive system and SARS-CoV‑2 infection. This review summarizes the manifestations and potential mechanisms of gastrointestinal manifestations in COVID‑19 in children.The aim of the work is a review of current data on the lesion of the gastrointestinal tract in children with COVID‑19 infection, and its impact on the management of patients with chronic diseases of the digestive tract.Materials and methods of research. Was carried out the analysis of publications in PubMed, EMBASE and Web of Science systems in the period from December 2019 to June 2020, documenting the clinical course of COVID‑19 disease with gastrointestinal lesions. The following search terms are used: “COVID‑19”, “SARS-CoV‑2”, “coronavirus”, “SARS-CoV‑2 pandemic, epidemic”, “outbreak”, “diarrhea”, “gastrointestinal symptoms”, “stool”, “feces” without any language restrictions. The main source of information was full-text scientific publications of both, foreign and russian authors.In addition, clinical cases of COVID‑19 were analyzed among patients of infectious departments of the Children’s Clinical Hospital named after Z. A. Bashlyaeva, Moscow. Clinical manifestations of the disease, data from laboratory and instrumental studies were evaluated. The most informative of them is given as a clinical example.
Formation of intestinal microbiocenosis in children born to mothers with gestational diabetes mellitus
Authors: Mayatskaya T. A. , Kharitonova L. A. , Papysheva O. V. , Zatevalov A. M. 
Published: March 2021

Rationale. The global medical community currently studies the influence of microbiocenosis on macroorganism. The metabolome of the human gut microbiome may be a factor in the development of pathology in tissues and organs.Objective. To improve the early diagnosis of intestinal microecology disorders in infants by determining the species composition and functional activity of the intestinal microflora to predict the health status of children born to mothers with diabetes.Materials and methods. We studied the intestinal microbiocenosis in 60 infants aged 1–28 days, including 22 infants by healthy mothers (control group) and 38 infants by mothers with diabetes (study group). The following was evaluated: the species composition of the intestinal microbiocenosis; its functional state by concentrations of short-chain fatty acids (SCFA); the content of elastase and simple carbohydrates in feces. The species composition was studied by fecal next generation sequencing (NGS), SCFA concentration was studied by gas-liquid chromatography of acidified fecal supernatant, pancreatic elastase in fecal samples was quantified by ELISA, and fecal carbohydrate content was determined by Benedict’s test. The gut dysbiosis was measured by the biodiversity of the microbial community quantified in biology by Shannon index according to the formula: where and corresponds to the number of microbial species in the intestinal microbial community. Normalized Shannon index has a range of values from 0 to 1, convenient for the interpretation of the microbiocenosis condition. An increase in the biodiversity of the microbial community was associated with an increase in dysbiotic changes of the gut microbiocenosis. Statistical analysis was performed with Statistica 8.0 and MS Office Excel 2010.Results. In children born by mothers with diabetes, the formation of microbial community at the stage of intestinal tract settlement occurs because of the increase of species biodiversity and formation of strong correlation between different classes of microorganisms, indicating a decrease of symbiont relationships of microbiocenosis with macroorganism normally mediated by intestinal epithelial cells condition and cooperation of the cells with intestinal mucosal barrier components. Due to the pronounced biodiversity and interspecies symbiosis of bacteria, compensatory mechanisms are formed in the colon for coexistence of macroorganism and microbiota. Thus, not only the butyric acid is produced in sufficient quantities but also the microbiota consumes SCFA releasing an increased amounts of iso-acids and promoting the growth of putrefactive microbial flora.
Clinical and Molecular Spectrum Associated with COL6A3 c.7447A>G p.(Lys2483Glu) Variant: Elucidating its Role in Collagen VI-related Myopathies
Authors: Villar-Quiles Rocío N. , Donkervoort Sandra , de Becdelièvre Alix , Gartioux Corine , Jobic Valérie , Foley A. Reghan , McCarty Riley M. , Hu Ying , Menassa Rita , Michel Laurence , Gousse Gaelle , Lacour Arnaud , Petiot Philippe , Streichenberger Nathalie , Choumert Ariane , Declerck Léa , Urtizberea J.A. , Sole Guilhem , Furby Alain , Cérino Matthieu , Krahn Martin , Campana- Salort Emmanuelle , Ferreiro Ana , Eymard Bruno , Bönnemann Carsten G. , Bharucha-Goebel Diana , Sumner Charlotte J. , Connolly Anne M. , Richard Pascale , Allamand Valérie , Métay Corinne , Stojkovic Tanya 
Published: March 2021

Background: Dominant and recessive autosomal pathogenic variants in the three major genes ( COL6A1-A2-A3) encoding the extracellular matrix protein collagen VI underlie a group of myopathies ranging from early-onset severe conditions (Ullrich congenital muscular dystrophy) to milder forms maintaining independent ambulation (Bethlem myopathy). Diagnosis is based on the combination of clinical presentation, muscle MRI, muscle biopsy, analysis of collagen VI secretion, and COL6A1-A2-A3 genetic analysis, the interpretation of which can be challenging. Objective: To refine the phenotypical spectrum associated with the frequent COL6A3 missense variant c.7447A>G (p.Lys2483Glu). Methods: We report the clinical and molecular findings in 16 patients: 12 patients carrying this variant in compound heterozygosity with another COL6A3 variant, and four homozygous patients. Results: Patients carrying this variant in compound heterozygosity with a truncating COL6A3 variant exhibit a phenotype consistent with COL6-related myopathies (COL6-RM), with joint contractures, proximal weakness and skin abnormalities. All remain ambulant in adulthood and only three have mild respiratory involvement. Most show typical muscle MRI findings. In five patients, reduced collagen VI secretion was observed in skin fibroblasts cultures. All tested parents were unaffected heterozygous carriers. Conversely, two out of four homozygous patients did not present with the classical COL6-RM clinical and imaging findings. Collagen VI immunolabelling on cultured fibroblasts revealed rather normal secretion in one and reduced secretion in another. Muscle biopsy from one homozygous patient showed myofibrillar disorganization and rimmed vacuoles. Conclusions: In light of our results, we postulate that the COL6A3 variant c.7447A>G may act as a modulator of the clinical phenotype. Thus, in patients with a typical COL6-RM phenotype, a second variant must be thoroughly searched for, while for patients with atypical phenotypes further investigations should be conducted to exclude alternative causes. This works expands the clinical and molecular spectrum of COLVI-related myopathies.
Konya Ekolojik Koşullarında Farklı Şeker Mısır (Zea mays saccharata Sturt) Genotiplerinin Verim ve Verim Komponentlerinin Belirlenmesi
Authors: TEZEL Mehmet , GÖNÜLAL Erdal , ARICI Ramazan Çağatay , ÖZCAN Gazi 
Published: March 2021

Bu çalışma Konya ekolojik koşullarında bazı şeker mısır genotiplerinin verim ve tarımsal özelliklerini belirlemek amacıyla iki yıl (2018-2019) süreyle yürütülmüştür. Tesadüf blokları deneme desenine göre üç tekerrürlü olarak yürütülen çalışmada 3 ticari çeşit (Batem Tatlı, Merit, Caramelo) ve 4 aday melez (ŞADA-1, ŞADA-12, ŞADA-16, ŞADA 42) olmak üzere toplam yedi genotip kullanılmıştır.
Çalışmada iki yılın ortalamaları üzerinden tane verimi 329 kg/da (Caramelo) - 690 kg/da (ŞADA-42), taze koçan verimi 847 kg/da (Caramelo) - 1259 kg /da (ŞADA-42), bitki boyu 128 cm (Caramelo) - 236 cm (ŞADA-42), çiçeklenme gün sayısı 69 gün (ŞADA-42)- 72.7 gün (BATEM Tatlı), koçan çapı 43.9 mm (ŞADA-12) -48.5 (Merit), koçan uzunluğu 15.2 cm (Merit)-19.6 cm (ŞADA-42), ilk koçan yüksekliği 35.2 cm (Caramelo)-79.8 cm (ŞADA-42), tane/koçan oranı % 80 (ŞADA-42)-% 84.9 (ŞADA-12), ve hasatta tane nemi% 12.6 (Caramelo)- % 14.9 (Batem Tatlı) aralığında elde edilmiştir. Çalışma sonucunda Orta Anadolu bölgesi için tane verimi ve yeşil koçan verimi açısından ŞADA-42 ve ŞADA-12 aday melezlerinin ön plana çıktığı görülmüştür.
Transcriptomic and Metabolomic Analysis Revealed Roles of Yck2 in Carbon Metabolism and Morphogenesis of Candida albicans
Authors: Liboro Karl , Yu Seong-Ryong , Lim Juhyeon , So Yee-Seul , Bahn Yong-Sun , Eoh Hyungjin , Park Hyunsook 
Published: March 2021

Candida albicans is a part of the normal microbiome of human mucosa and is able to thrive in a wide range of host environments. As an opportunistic pathogen, the virulence of C. albicans is tied to its ability to switch between yeast and hyphal morphologies in response to various environmental cues, one of which includes nutrient availability. Thus, metabolic flexibility plays an important role in the virulence of the pathogen. Our previous study has shown that C. albicans Yeast Casein Kinase 2 (CaYck2) regulates the yeast-to-hyphal switch, but its regulatory mechanisms remain unknown. This study further elucidated the role of Yck2 in governing morphology and carbon metabolism by analyzing the transcriptome and metabolome of the C. albicans YCK2 deletion mutant strain (yck2Δ strain) in comparison to the wild type strain. Our study revealed that loss of CaYck2 perturbs carbon metabolism, leading to a transcriptional response that resembles a transcriptional response to glucose starvation with coinciding intracellular accumulation of glucose and depletion of TCA cycle metabolites. This shift in the metabolome is likely mediated by derepression of glucose-repressed genes in the Mig1/2-mediated glucose sensing pathway and by downregulation of glycolytic genes, possibly through the Rgt1-mediated SRR pathway. In addition, genes involved in beta-oxidation, glyoxylate cycle, oxidative stress response, and arginine biosynthesis were upregulated in the yck2Δ strain, which is highly reminiscent of C. albicans engulfment by macrophages. This coincides with an increase in arginine degradation intermediates in the yck2Δ strain, suggesting arginine catabolism as a potential mechanism of CaYck2-mediated filamentation as seen during C. albicans escape from macrophages. Transcriptome analysis also shows differential expression of hyphal transcriptional regulators Nrg1 and Ume6. This suggests dysregulation of hyphal initiation and elongation in the yck2Δ strain which may lead to the constitutive pseudohyphal phenotype of this strain. Metabolome analysis also detected a high abundance of methyl citrate cycle intermediates in the yck2Δ strain, suggesting the importance of CaYck2 in this pathway. Taken together, we discovered that CaYck2 is an integral piece of carbon metabolism and morphogenesis of C. albicans.
Modeling of Urinary Microbiota Associated With Cystitis
Authors: Ceprnja Marina , Oros Damir , Melvan Ena , Svetlicic Ema , Skrlin Jasenka , Barisic Karmela , Starcevic Lucija , Zucko Jurica , Starcevic Antonio 
Published: March 2021

A decade ago, when the Human Microbiome Project was starting, urinary tract (UT) was not included because the bladder and urine were considered to be sterile. Today, we are presented with evidence that healthy UT possesses native microbiota and any major event disrupting its “equilibrium” can impact the host also. This dysbiosis often leads to cystitis symptoms, which is the most frequent lower UT complaint, especially among women. Cystitis is one of the most common causes of antimicrobial drugs prescriptions in primary and secondary care and an important contributor to the problem of antimicrobial resistance. Despite this fact, we still have trouble distinguishing whether the primary cause of majority of cystitis cases is a single pathogen overgrowth, or a systemic disorder affecting entire UT microbiota. There are relatively few studies monitoring changes and dynamics of UT microbiota in cystitis patients, making this field of research still an unknown. In this study variations to the UT microbiota of cystitis patients were identified and microbial dynamics has been modeled. The microbial genetic profile of urine samples from 28 patients was analyzed by 16S rDNA Illumina sequencing and bioinformatics analysis. One patient with bacterial cystitis symptoms was prescribed therapy based on national guideline recommendations on antibacterial treatment of urinary tract infections (UTI) and UT microbiota change was monitored by 16S rDNA sequencing on 24 h basis during the entire therapy duration. The results of sequencing implied that a particular class of bacteria is associated with majority of cystitis cases in this study. The contributing role of this class of bacteria – Gammaproteobacteria, was further predicted by generalized Lotka-Volterra modeling (gLVM). Longitudinal microbiota insight obtained from a single patient under prescribed antimicrobial therapy revealed rapid and extensive changes in microbial composition and emphasized the need for current guidelines revision in regards to therapy duration. Models based on gLVM indicated protective role of two taxonomic classes of bacteria, Actinobacteria and Bacteroidia class, which appear to actively suppress pathogen overgrowth.
Multiscale Risk Factors of Cardiovascular Disease: CLSA Analysis of Genetic and Psychosocial Factors
Authors: Menniti Gabriella , Paquet Catherine , Han Hannah Yang , Dube Laurette , Nielsen Daiva E. 
Published: March 2021

Background: Cardiovascular disease (CVD) is a complex disease resulting from multiscale risk factors including genetics, age, and psychosocial factors (PSFs) such as depression and social isolation. However, previous research has lacked in operationalizing multiscale risk factors to determine individual and interactive associations over the life course. Therefore, this study aimed to evaluate individual and interactive associations of multiscale risk factors for CVD outcomes including genetics and PSFs at middle and older-aged stages of the life course.Methods: Baseline data from the Canadian Longitudinal Study on Aging (CLSA; n = 9,892 with genome-wide genotyping data) was used for this investigation. A 39 single nucleotide polymorphism polygenic risk score (PRS) for CVD was constructed. PSFs consisted of: (1) Depressive symptoms categorized into: “none” (Group 1, reference), “current” (Group 2), “clinical depression with no current symptoms” (Group 3), and “potential, recurrent depression” (Group 4); and (2) Social isolation index as a binary variable comprised of marital status, living arrangements, retirement status, contacts, and social participation. Heart-related disorders (HRD: myocardial infarction, angina and heart disease) was the primary outcome of interest and peripheral/vascular-related disorders (PVRD: stroke, peripheral vascular disease and hypertension) was the secondary outcome. Multivariable logistic regression models adjusted for socio-demographic factors were conducted stratified by age group (middle-aged: 45–69 years, older-aged: ≥70 years).Results: PRS was associated with HRD among middle- and older-aged participants [OR (95% confidence interval)] [1.06 (1.03–1.08), 1.06 (1.03–1.08), respectively]. Most depressive symptoms groups compared to the reference associated with HRD and PVRD, but only Group 4 associated with PVRD among older-aged [1.69 (1.08–2.64)]. Social isolation was associated with only PVRD among middle-aged [1.84 (1.04–3.26)]; however, socially isolated CLSA participants were underrepresented in the genotyped cohort (1.2%). No significant PRS*PSFs interactions were observed.Conclusions: Genetics and PSFs are independently associated with CVD. Varying observations across age groups underscores the need to advance research on multiscale risk factors operating both at a given point in time and over the life course. Future cohort studies may benefit from use of mobile assessment units to enable better reach to socially isolated participants for collection of biospecimens.