bluuebunny/crossref_metadata_2025_split huggingface dataset:
Activated Coagulation Factor X: A Novel Mitogenic Stimulus for Human Mesangial Cells
Authors: MONNO RAFFAELLA , GRANDALIANO GIUSEPPE , FACCIO ROBERTA , RANIERI ELENA , MARTINO CARMELA , GESUALDO LORETO , SCHENA FRANCESCO P. 
Published: April 2021

Abstract.
Intraglomerular activation of the coagulation cascade is a common feature of mesangioproliferative glomerulonephritis. Besides thrombin, very little is known about the cellular effects of other components of the coagulation system. This study investigated the effect of activated factor X (FXa) on cultured human mesangial cells. This serine protease induced a significant and dose-dependent increase in DNA synthesis. In addition to its mitogenic effect, FXa caused a striking upregulation of platelet-derived growth factor (PDGF) A and B chain gene expression. Next, the intracellular mitogenic signaling pathways activated by FXa were investigated. FXa induced a rapid spike in cytosolic calcium concentration followed by a sustained plateau. This response was not influenced by the downregulation of thrombin receptors. In addition, FXa stimulated a significant upregulation of different tyrosine-phosphorylated proteins. One of these phosphorylated cellular proteins was represented by the c-jun N-terminal kinase, a member of the mitogen-activated protein kinase family. To evaluate the role of FXa enzymatic activity and of PDGF autocrine secretion, FXa-induced DNA synthesis was studied in the presence of leupeptin, a specific serine protease inhibitor, and neutralizing anti-PDGF antibody. To investigate the role of tyrosine kinase (TK) activation on FXa mitogenic effect, FXa-stimulated thymidine uptake was evaluated in the presence of genistein and herbimycin A, two powerful and specific TK inhibitors. FXa-elicited DNA synthesis was also examined after protein kinase C (PKC) downregulation by prolonged incubation with phorbol-12-myristate-13-acetate to study the influence of the phospholipase C-PKC axis. The proliferative effect of FXa required its proteolytic activity, and the activation of TK was only partially dependent on PKC activation while it was PDGF independent. Finally, it was shown by reverse transcription-PCR that mesangial cells do not express the signaling splicing variant of the putative FXa receptor, effector protease receptor-1. In conclusion, the present study demonstrated that FXa is a powerful mitogenic factor for human mesangial cells, and it induces its cellular effect not through effector protease receptor-1, but most likely by binding a protease-activated receptor and activating phospholipase C—PKC and TK signaling pathways.
Role of the Increase in p21 in Cisplatin-Induced Acute Renal Failure in Rats
Authors: MIYAJI TAKEHIKO , KATO AKIHIKO , YASUDA HIDEO , FUJIGAKI YOSHIHIDE , HISHIDA AKIRA 
Published: April 2021

Abstract.
The goal of this study was to clarify the role of p21, a cyclin-dependent kinase inhibitor, in acute renal failure (ARF). This was accomplished with the examination of the renal expression of p21 in cisplatin (CDDP)-induced ARF and in rechallenge injury with CDDP. The injection of CDDP (5 mg/kg) into rats induced increases in serum creatinine and tubular damage and the number of in situ DNA nick end labeling—positive cells, which peaked at day 5, followed by recovery to control levels by day 14. The rechallenge with the same dose of CDDP 14 d after the first dose of CDDP induced significantly less injury and no significant increase in in situ DNA nick end labeling—positive cells. The first CDDP dose significantly increased p53-positive nuclei at day 1, which disappeared by day 5, and the number of p21-positive nuclei, which had two peaks on days 3 and 9. The number of proliferating cell nuclear antigen (PCNA)-positive nuclei peaked at days 3 and 12. A significant increase in the incorporation of 5-bromo 2′-deoxyuridine (BrdU) was found at day 5 and peaked at day 7. The second injection of CDDP induced significant increases in the number of p21-, p53-, and PCNA-positive nuclei within 2 d but did not affect the incorporation of BrdU. These findings suggested that (1) CDDP induced two peaks of the increase in p21; (2) the first peak occurred shortly after CDDP and was accompanied by overexpression of p53 and PCNA but not with BrdU incorporation, possibly reflecting G1 arrest and DNA repair; (3) the second peak of p21 occurred through an p53-independent pathway and may contribute to cell differentiation; and (4) the overexpression of p21 and PCNA in rechallenge injury may contribute to acquired resistance in CDDP-induced ARF via enhanced DNA repair.
Calcitriol Controls the Epithelial Calcium Channel in Kidney
Authors: HOENDEROP JOOST G. J. , MU[Combining Diaeresis]LLER DOMINIK , VAN DER KEMP ANNEMIETE W. C. M. , HARTOG ANITA , SUZUKI MAKOTO , ISHIBASHI KENICHI , IMAI MASASHI , SWEEP FRED , WILLEMS PETER H. G. M. , OS CAREL H. VAN , BINDELS RENÉ J. M. 
Published: April 2021

Abstract.
The recently cloned epithelial Ca2+ channel (ECaC), which is expressed primarily in 1,25-dihydroxyvitamin D3 (1,25(OH)2D3)-responsive Ca2+ -transporting epithelia, is postulated to constitute the rate-limiting step in active Ca2+ reabsorption. In the present study, the effect of 1,25(OH)2D3 was investigated on ECaC mRNA and protein levels in kidneys of rats that were raised on a vitamin D—depleting diet. This diet decreased the serum 1,25(OH)2D3 concentration significantly, which was accompanied by a marked drop in serum Ca2+ level. Both 1,25(OH)2D3 and Ca2+ levels were normalized within 48 h after 1,25(OH)2D3 administration. In 1,25(OH)2D3-deficient rats, ECaC mRNA and protein levels of the kidney cortex were significantly decreased compared with the repleted animals, suggesting that 1,25(OH)2D3 exerts its stimulatory effect on Ca2+ reabsorption via increased ECaC expression. In agreement with this observation, the elucidated human ECaC promoter contains several consensus vitamin D—responsive elements. ECaC was restricted to the apical membrane of the distal part of the distal convoluted and the connecting tubule. This conclusion was based on only minor overlap with the localization of the thiazide-sensitive NaCl co-transporter and complete co-localization with the 1,25(OH)2D3-dependent Ca2+ binding protein, calbindin-D28K. In conclusion, ECaC, present in the distal part of the nephron, is an important target for 1,25(OH)2D3-mediated Ca2+ reabsorption.
Renal Monoclonal Immunoglobulin Deposition Disease
Authors: LIN JULIE , MARKOWITZ GLEN S. , VALERI ANTHONY M. , KAMBHAM NEERAJA , SHERMAN WILLIAM H. , APPEL GERALD B. , D'AGATI VIVETTE D. 
Published: April 2021

Abstract.
This study reports the clinicopathologic findings and outcome in 34 patients with renal monoclonal immunoglobulin deposition disease (MIDD), which included 23 light-chain DD (LCDD), 5 light- and heavy-chain DD (LHCDD), and 6 heavy-chain DD (HCDD). A total of 23 patients had pure MIDD, whereas 11 patients had LCDD with coexistent myeloma cast nephropathy (LCDD & MCN). Renal biopsy diagnosis preceded clinical evidence of dysproteinemia in 68% of all cases. By immunofluorescence, the composition of deposits included 11κ/1λ (LCDD), 3IgGκ/2IgGλ (LHCDD), 5γ/1α (HCDD), and 10κ/1λ (LCDD & MCN). Patients with pure MIDD presented with mean serum creatinine of 4.2 mg/dl, nephrotic proteinuria, and hypertension. Cases of HCDD were associated with a CH1 deletion and frequently had hypocomplementemia and a positive hepatitis C virus antibody but negative hepatitis C virus PCR. LCDD & MCN is a morphologically and clinically distinct entity from pure MIDD, presenting with higher creatinine (mean, 7.8 mg/dl; P = 0.01), greater dialysis dependence (64 versus 26%; P = 0.053), subnephrotic proteinuria, and less nodular glomerulopathy (18 versus 100%; P < 0.0001). Multiple myeloma was more frequently diagnosed in LCDD & MCN than in pure MIDD (91 versus 31%; P = 0.025). Renal and patient survivals were significantly worse in patients with LCDD & MCN (mean, 4 and 22 mo, respectively), compared with patients with pure MIDD (mean, 22 and 54 mo). Chemotherapy stabilized or improved renal function in 10 of 15 patients (67%) with pure MIDD who presented with creatinine of <5.0 mg/dl, emphasizing the importance of early detection. On multivariate analysis, initial creatinine was the only predictor of renal and patient survival in pure MIDD, underscoring the prognostic significance of the renal involvement.
TURİZM GELİRLERİNİN CARİ İŞLEMLER DENGESİNE ETKİLERİ: TÜRKİYE ÖRNEĞİ ÜZERİNDE YAPAY SİNİR AĞLARI İLE ÖNGÖRÜ
Authors: CİNEL Emek Aslı , YOLCU Ufuk 
Published: April 2021

Küreselleşen yeryüzünde önemi her geçen gün artan turizm sektörü, döviz geliri, dış ödemeler dengesi üzerindeki olumlu etkisi, meydana getirdiği istihdam ve vergi gelirleriyle ekonomiyi pozitif yönde etkileyen bir faaliyet alanıdır. Farklı birçok hizmet türünü içerisine alan turizm, ekonomide üretim ve tüketim artışına neden olmaktadır. Farklı birçok sektörü kolayca etkileyebildiği için ülkelerin öncelik verdiği, hükümetlerin çeşitli politika ve uygulamalarla daha fazla geliştirmeyi hedeflediği bir sektör pozisyonundadır.
Gelişmekte olan birçok ülke ile benzer biçimde ülkemizde de turizm sektörünün ilerlemesi ekonomik büyümenin sağlanabilmesi bakımından büyük önem taşımaktadır. Turizm gelirleri, ülke ekonomilerine döviz girdisi sağlamakta ve ödemeler dengesini doğrudan etkilemektedir. Bu bağlamda çalışmada, turizm sektörünün dış ticaret dengesi üzerinde yarattığı etkilerin ortaya konulması amaçlanmış, turizm gelirleriyle dış ticaret dengesi arasındaki ilişki yapay sinir ağları yöntemiyle öngörülmeye çalışılmıştır. 2013-2020 dönemini kapsayan dış ticaret dengesi öngörüsünde yapay sinir ağı girdileri olarak aynı döneme ilişkin turizm gelirleri model girdileri olarak kullanılmış ve çözümlemeler, TÜİK’ten elde edilen aylık verilerle gerçekleştirilmiştir.
Zapalenie starcze - mechanizmy i szlaki sygnałowe
Authors: Grabowska Konstancja , Nowacka-Chmielewska Marta , Barski Jarosław , Liskiewicz Daniela 
Published: April 2021

Starzenie się jest złożonym i wieloetapowym procesem, angażującym mechanizmy na poziomie molekularnym, komórkowym i tkankowym. Podczas procesu starzenia się obserwowany jest rozwój przewlekłego, sterylnego stanu zapalnego, który jest określany terminem inflammaging, czyli zapalenie starcze. Zapalenie starcze jest wymieniane jako czynnik ryzyka wystąpienia i progresji chorób przewlekłych, nie tylko tych związanych z wiekiem. Ponadto, zapalenie starcze sprzyja wzrostowi zachorowalności i śmiertelności oraz wpływa na jakość życia osób starszych. Do rozwoju zapalenia starczego przyczynia się starzenie komórkowe oraz zaburzenia w regulacji aktywacji inflamasomów, pracy mitochondriów, autofagii i mitofagii, działania systemu ubikwityna â proteasom i odpowiedzi na uszkodzenia DNA. Powyższe procesy wzajemnie na siebie oddziaływają oraz są modulowane poprzez komórkowe szlaki sygnałowe uczestniczące w regulacji odpowiedzi zapalnej tj.: szlak mTOR, RIG-I, Notch, TGF-b, Ras, oraz regulacja aktywności sirtuin. Szczególną uwagę przypisuje się czynnikowi transkrypcyjnemu NF-kB. Celem pracy jest przedstawienie procesów oraz szlaków sygnałowych leżących u podstaw zapalenia starczego z uwzględnieniem badań klinicznych i eksperymentalnych.
A transformação das formas
Authors: Azevedo Aina Guimarães 
Published: April 2021

A Land Reform na África do Sul dos dias atuais é analisada em termos dos significados que a terra tem para as pessoas negras falantes de isiZulu, em oposição ao que fazendeiros brancos consideram modelos de fracasso. As casas, experienciadas ao longo de vidas marcadas pelo desterro e por mudanças, são apreciadas em sua expressão de conquista, através da história da família Kubheka, contemplada, pelo programa fundiário sul-africano, com uma fazenda (anteriormente de brancos) para morar. A forma das casas é aqui questionada sob a “perspectiva da morada” de Ingold, quando atentamos para o que as pessoas fazem nas casas onde moram para que as considerem lares. Nesse sentido, rituais que relacionam pessoas, ancestrais e casas, como o casamento, surgem como imperativos a serem realizados. Ao lidarem com as diversas formas que pessoas e casas adquirem nas mudanças de moradias e nas performances rituais – que, de algum modo, ao restaurar pessoas e casas às suas formas originais, reinventam as formas atuais –, percebemos a criatividade no processo de morar e de transformar casas em lugares considerados próprios para se viver, nos termos daquilo que as pessoas fazem, ou desejam fazer, e não daquilo que deveriam fazer segundo olhares alheios.
A Novel Method and its Application to Define Maximum Horizontal Stress and Stress Path
Authors: Zhang Jianguo , Mahadev Karthik , Edwards Stephen , Rodgerson Alan 
Published: April 2021

Abstract
Maximum horizontal stress (SH) and stress path (change of SH and minimum horizontal stress with depletion) are the two most difficult parameters to define for an oilfield geomechanical model. Understanding these in-situ stresses is critical to the success of operations and development, especially when production is underway, and the reservoir depletion begins. This paper introduces a method to define them through the analysis of actual minifrac data. Field examples of applications on minifrac failure analysis and operational pressure prediction are also presented.
It is commonly accepted that one of the best methods to determine the minimum horizontal stress (Sh) is the use of pressure fall-off analysis of a minifrac test. Unlike Sh, the magnitude of SH cannot be measured directly. Instead it is back calculated by using fracture initiation pressure (FIP) and Sh derived from minifrac data. After non-depleted Sh and SH are defined, their apparent Poisson's Ratios (APR) are calculated using the Eaton equation. These APRs define Sh and SH in virgin sand to encapsulate all other factors that influence in-situ stresses such as tectonic, thermal, osmotic and poro-elastic effects. These values can then be used to estimate stress path through interpretation of additional minifrac data derived from a depleted sand.
A geomechanical model is developed based on APRs and stress paths to predict minifrac operation pressures. Three cases are included to show that the margin of error for FIP and fracture closure pressure (FCP) is less than 2%, fracture breakdown pressure (FBP) less than 4%. Two field cases in deep-water wells in the Gulf of Mexico show that the reduction of SH with depletion is lower than that for Sh.
The First Assessment on Metal Contamination in the Critically Endangered Sawback Angelshark (Squatina Aculeata) From North-Eastern Mediterranean
Authors: TURAN funda , YOLA Mehmet Lütfi , ERGENLER Aysegul , TURAN Cemal 
Published: April 2021

Abstract
A Sawback angelshark (Squatina aculeata) classified as “Critically Endangered” Global Red List by the IUCN (2017) and CITES (2017) is one of the threated elasmobranch groups and the increased pollution in the marine environment negatively impact angelshark species. Despite this, limited information exists about the effects of toxic chemical contamination in angelsharks. Therefore, present study was conducted to elucidate heavy metal accumulation in Squatina aculeata captured from the Iskenderun Bay, North-Eastern Mediterranean by electrochemical technique. Arsenic (As), Mercury (Hg), Copper (Cu), Iron (Fe), Zinc (Zn) and Mangan (Mn) were found by electrochemical analyzer. These results showed that Fe and Zn were the highest concentrations in all tissues of S. aculeata and, the accumulations of Hg, Fe, Zn and Mn were significantly different in the gill, liver and muscle tissues (P < 0.05). As and Hg concentrations exceeded maximum limit legalized by FAO (2003) and UNEP (2008). This results confirmed that the metal contamination in North-Eastern Mediterranean area may pose a potential threat to the sustainability of S. aculeata in marine ecosystem
ECC-Based Lightweight Authentication And Access Control Scheme For IoT E-Healthcare
Authors: Yao Hailong , Yan Qiao , Fu Xingbing , Zhang Zhibin , Lan Caihui 
Published: April 2021

Abstract
The E-healthcare system has a complex architecture, diverse business types, and sensitive data security. To meet the secure communication and access control requirements in the user-medical server, user-patient, patient-medical server and other scenarios in the E-healthcare system, secure and efficient authenticated key agreement and access authorization scheme need to be studied. However, the existing multi-server solutions do not consider the authentication requirements of the Wireless Body Area Network(WBAN), and are not suitable for user-patient, patient-medical server scenarios; most of the existing WBAN authentication scheme are single-server type, which are difficult to meet the requirements of multi-server applications; the study of user-patient real-time scenarios has not received due attention. This work first reveals the structural flaws and security vulnerabilities of the existing typical schemes, and then proposes an authentication and access control architecture suitable for multiple scenarios of the E-healthcare system with separate management and business, and designs a novel ECC-based multi-factor remote authentication and access control scheme for E-healthcare using physically uncloneable function (PUF) and hash. Security analysis and efficiency analysis show that the new scheme has achieved improved functionality and higher security while maintaining low computational and communication overhead.
Comparison of Radiation-Induced DNA Damage between Conventional and Computed Tomography Coronary Angiography
Authors: Gökalp Gökhan , Ünlü Serkan , Elkama Aylin , Yalçın Alican , Cemri Mustafa , Karahalil Bensu , Erbaş Gonca , Boyacı Nuri Bülent 
Published: April 2021

Abstract
Purpose: Conventional coronary angiography (CCA) and coronary computed tomography angiography (CCTA) are the most frequently used imaging modalities to diagnose coronary artery disease (CAD). The amount of radiation and genotoxic damages of these imaging methods showed variation with the improved technology. Thus we sought to compare the ionizing radiation doses and radiation-induced DNA damage in patients who were performed CCA and CCTA.Methods: 76 patients (39 in CCA group, 37 in CCTA group) were enrolled. Patients undergoing CCTA were grouped according to the use of flash technique (22 patients with CCTA-flash, 15 patients with CCTA-other). The effective radiation dose was recorded. Genotoxicity was compared with chromosome aberration test before and after imaging methods.Results: There was a significant difference between the groups in effective radiation doses given to patients. Radiation was lowest in the CCTA-flash group, followed by CCA, and non-flash CCTA group. There was no change in chromosome aberration rate after CCTA-flash group (p = 0.479). There was a significant increase in chromosome aberration rates after CCA and CCTA-other groups (CCA: p = 0.001; CCTA-other: p = 0.01). Conclusions: CTA which was taken with flash technique in dual-energy CT devices delivers lower dose radiation compared to other groups. Due to this significant difference, radiation-induced genetic damage was significantly less in patients with CCTA undergoing flash technique.
Evaluation of the effects of minimum winter temperatures on changing the type of cultivation and changing the use of agricultural lands. (Case study: Urmia county)
Authors: farahmand ghasem , khaledi shariar , tali manijeh ghahroudi 
Published: April 2021

Abstract
Land use and land cover change (LULC) and climate change are among the major threats to the global environment. Assessing the causes of land use change and its relationship with climate change is one of the important issues that understanding its process can help better human interaction with the environment. Therefore, the purpose of this study is to evaluate the effects of climate change on changing land use and land cover. The indicators used to achieve the mentioned goal are: ((average minimum winter temperatures), Number of days (≤ 0°C), Number of days (≤-10°C) and 30-year Landsat satellite images)), CMIP5(CanESM2) model was used to predict temperature changes and CA-MARKOV model was used to predict land use changes and finally Pearson correlation coefficient test was used to measure the correlation. The results of the study indicated that, there is a direct relationship between changes of minimum winter temperatures and changing the type of cultivation and land use in Urmia city. Also, simulation of temperature changes showed that there is the highest (> 0.8) correlation between rcp4.5 scenarios and land use changes, which indicates a high probability of changes in the specified time period (2018–2033).
An Auction Based Task Dispatching and Pricing Mechanism in Bike-sharing
Authors: Shi Bing , Deng Yaping , Yuan Han 
Published: April 2021

Abstract
As a green and low-carbon transportation way, bike-sharing provides lots of convenience in the daily life. However, the daily usage of sharing bikes results in dispatching problems, i.e. dispatching bikes to the specific destinations. The bike-sharing platform can hire and pay to workers in order to incentivize them to accomplish the dispatching tasks. However, there exist multiple workers competing for the dispatching tasks, and they may strategically report their task accomplishing costs (which are private information only known by themselves) in order to make more profits, which may result in inefficient task dispatching results. In this paper, we first design a dispatching algorithm named GDY-MAX to allocate tasks to workers, which can achieve good performance. However it cannot prevent workers strategically misreporting their task accomplishing costs. Regarding this issue, we further design a strategy proof mechanism under the budget constraint, which consists of a task dispatching algorithm and a worker pricing algorithm. We theoretically prove that our mechanism can satisfy the properties of incentive compatibility, individual rationality and budget balance. Furthermore we run extensive experiments to evaluate our mechanism based on a dataset from Mobike. The results show that the performance of the proposed strategy proof mechanism and GDY-MAX is similar to the optimal algorithm in terms of the coverage ratio of accomplished task regions and the sum of task region values, and our mechanism has better performance than the uniform algorithm in terms of the total payment and the unit cost value.