bluuebunny/crossref_metadata_2025_split huggingface dataset:
Beta-Diketone–Cobalt Complexes Sensitize Glioma Stem Cells to Temozolomide Partly Through the ERK-MGMT Pathway
Authors: Zhang Kaizhi , Lang Fang , Mang Jing 
Published: August 2021

Abstract
Glioblastoma multiforme is characterized by high invasiveness, multidrug resistance, and inevitable recurrence, and current standard treatment regimens are not curative. Even if most glioma cells are eliminated by chemotherapy and radiotherapy, glioma stem cells can survive and differentiate into new tumor cells, thereby triggering tumor recurrence and drug resistance. Therefore, inhibiting tumor invasiveness, reversing drug resistance, and effectively ablating glioma stem cells are critical for improving the prognosis of glioblastoma multiforme. Previous studies reported that the combination of β-diketone–cobalt complexes (CoAc2) and temozolomide (TMZ) has synergistic inhibitory effects on glioma cells. Therefore, we compared cell proliferation, colony-forming capacity, cell migration, and invasion of TMZ-resistant glioma cells and corresponding glioma stem cells after treatment with CoAc2 and/or TMZ. We also explored the underlying mechanism by which CoAc2 sensitizes cells to TMZ through transcriptome sequencing and related signal pathway blockade. We found that CoAc2 significantly increased the inhibitory effect of TMZ on the proliferation, colony formation, migration, invasion, and survival of drug-resistant stem cells. By downregulating ERK pathway activity, CoAc2 inhibited the expression of O6-methylguanine-DNA methyltransferase and eventually sensitized drug-resistant glioma cells to TMZ. In conclusion, the combined use of CoAc2 and TMZ can reverse TMZ resistance and significantly enhance its inhibitory effect on the malignant phenotype of glioma cells and glioma stem cells.
Construction and Validation of a Prognostic Model Based on 11 Lymph Node Metastasis Related Genes for Overall Survival in Endometrial Cancer
Authors: Wu Hong , Feng haiqin , Miao Xiaoli , Ma jiancai , Liu Cairu , Zhang Lina , Yang Liping 
Published: August 2021

Abstract

Background: Endometrial cancer (EC) is one of the most common malignant tumor in the female reproductive system. The incidence of lymph node metastasis (LNM) is only about 10% in clinically suspected early-stage EC patients. Discovering prognostic model and effective biomarkers for early diagnosis is important to reduce the mortality rate. Methods: We downloaded the RNA-sequencing data and clinical information from the TCGA database. Gene ontology (GO) enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were used to analyze the differentially expressed genes (DEGs). A least absolute shrinkage and selection operator (LASSO) regression was conducted to identify the characteristic dimension decrease and distinguish prognosis-related LNM related genes signature. Subsequently, a novel prognosis-related nomogram to predict overall survival (OS). A survival analysis was carried out to explore the individual prognostic significance of the risk model and key gene was validated in vitro. Results: In total, 89 LRGs were identified. Based on the LASSO Cox regression, 11 genes were selected for the development of a risk evaluation model. The Kaplan–Meier curve indicated that patients in the low-risk group had considerably better OS (P = 3.583e−08). The area under the curve (AUC) of this model was 0.718 at 5 years of OS. Then, we developed an OS-associated nomogram that included the risk score and clinicopathological features. The concordance index of the nomogram was 0.769. The survival verification performed in three subgroups from the nomogram demonstrated the validity of the model. The AUC of the nomogram was 0.787 at 5 years OS. Proliferation and metastasis of HMGB3 were explored in EC cell line. Finally, we revealed that the most frequently mutated genes in the low-risk and high-risk groups are PTEN and TP53, respectively. Conclusions: Our results suggest that LNM plays an important role in the prognosis, and HMGB3 was potential as a biomarker for EC patients. By detecting the mutation of the risk signature, clinicians can accurately treat patients with targeted therapy, thereby improving their survival rate.
ERAS Improves Postoperative Recovery in Children
Authors: Wu Tingmei , Li Haiwen , Zhou Huixia , Hao Xuemei , Wang Xiaojun , Lu Yan , Ge Wenchao , Li Xiangpeng , Li Dongming , Li Wei , Ma Lifei , Tao Tian , Zhou Xiaoguang , Ma Yaqun , Guo Hang 
Published: August 2021

Abstract
Objective: Enhanced recovery after surgery (ERAS) protocols are established in adults but not fully evaluated in children. This study investigated whether an ERAS protocol improved recovery and influenced postoperative inflammatory cytokine levels in children undergoing surgery for hydronephrosis. Methods: This randomized controlled study included patients who underwent robot-assisted laparoscopic surgery for hydronephrosis at Bayi Children's Hospital (Beijing, China) between October 2018 and September 2019. Patients were randomized to an ERAS group (perioperative ERAS protocol) or control group (standard perioperative management). Outcomes related to postoperative recovery and inflammatory cytokine levels were evaluated. Results: The final analysis included 18 patients in each group. Five patients (27.78%) in each group experienced postoperative complications (abdominal pain, nausea and vomiting, subcutaneous emphysema or fever). The ERAS group had a shorter time to first postoperative flatus than the control group (25 vs. 49 hours; P =0.009), although the time for abdominal drainage flow to reach ≤20 mL/day, time to urinary catheter removal and length of hospital stay did not differ significantly between groups. Preoperative plasma cytokine levels were comparable between groups. Compared with the control group, the ERAS group had a higher IL-6 level on postoperative day 2 ( P <0.05) and a lower concentration of IL-1β on postoperative days 1 and 2 ( P <0.05). Postoperative levels of CRP, TNFα and IL-10 did not differ significantly between groups. Conclusions: ERAS may accelerate postoperative recovery and modulate the postoperative inflammatory response in pediatric patients undergoing robot-assisted laparoscopic pyeloplasty for hydronephrosis.
The Influece of The Laser on Acoustic Phonon Amplification in Parabolic Potential Well
Authors: Tien Dung Nguyen , Thanh Thuy Do , Canh Trung Le 
Published: August 2021

Abstract
We present a theoretical study of the kinetic equation for acoustic phonons in semiconductor quantum well with parabolic potential well under intense laser field. Using this method, we find the expression for the phonon rate coefficient for the general case, the condition of the acoustic phonon rate and the influence of the parameters of laser on this rate coefficient. We numerically calculate the rate of acoustic phonon excitation by the absorption of laser field energy at different temperature.
Circular RNA Hsa_Circ_0002483 Promotes Growth and Invasion of Lung Adenocarcinoma by Sponging miR-125a-3p
Authors: Wan Jun , Ding Guanggui , Zhou Min , Ling Xiean , Rao ZhanPeng 
Published: August 2021

Abstract
Background: Increasing evidence indicates that the aberrant expression of circular RNAs (circRNAs) is involved in the pathogenesis and progression of lung adenocarcinoma (LUAC). However, the function and molecular mechanisms of hsa_circ_0002483 (circ_0002483) in LUAC remain unclear. Methods: The association between circ_0002483 expression and clinicopathological characteristics and prognosis in patients with LUAC was analyzed by fluorescence in situ hybridization. The functional experiments such as MTT, colony formation and Transwell assays and a subcutaneous tumor model were conducted to determine the role of circ_0002483 in LUAC cells. The specific binding between circ_0002483 and miR-125a-3p was validated by RNA immunoprecipitation, luciferase gene report and qRT-PCR assays. The effects of circ_0002483 on miR-125a-3p-mediated C-C motif chemokine ligand 4 (CCL4)-CCR5 axis were assessed by Western blot analysis.Results: We found that circ_0002483 was upregulated in LUAC tissue samples and associated with TNM stage and poor survival in patients with LUAC. Knockdown of circ_0002483 inhibited proliferation, colony formation and invasion of A549 and PC9 cells in vitro, whereas overexpression of circ_0002483 harbored the opposite effects. Furthermore, circ_0002483 sponged miR-125a-3p and negatively modulated its expression. CCL4 was identified as a direct target of miR-125a-3p. The rescue experiments showed that miR-125a-3p mimics reversed the tumor-promoting effects of circ_0002483 by targeting CCL4-CCR5 axis in A549 and PC9 cells. In addition, the in vivo experiment further validated that knockdown of circ_0002483 repressed tumor growth. Conclusions: Our findings demonstrated that circ_0002483 could act as a sponge of miR-125a-3p to upregulate CCL4-CCR5 axis, contributing to the tumorigenesis of LUAC, and represent a potential therapeutic target for LUAC.
Unidentified Persons With Disabilities in India- A Study Based on 76th Round of NSS (2018-19) Data
Authors: Mishra Radhe Shyam , Mohanty Sanjay K , Chanda Srei 
Published: August 2021

Abstract
Background: Identification of the disabled and providing them a disability certificate is the first step for provisioning educational, employment, and other social benefits in a welfare state. Deprivation from disability certificate deprives persons with disability (PwDs) from government policy and programme. Though studies examined the extent, variation, and pattern of disability, no attempt has been made in understanding the extent and variation of unidentified PwDs in India. The study aims to examine the variation in unidentified PwDs by type of disability, state and socio-economic correlates.Data and methods: The unit data from the 76th round (schedule 26) of Nation Sample Survey, 2018 is used in analyses. A total of 1,06,894 disabled across seven types of disabilities; any disability, locomotor, visual, hearing, speech, mental retardation, mental illness, and other disability have been analysed. Descriptive statistics and logistic regression analyses are used.Results: 7 in 10 disabled person in India don’t have a disability certificate. The percentage share of PwDs with a disability certificate varies 12% among those with hearing disability to 47% with mental retardation. Unadjusted odds ratio shows that person with hearing disability (OR=0.30 CI 0.303-0.304) are less likely to hold a disability certificate compare to locomotor disability followed by mental illness (OR=0.52 CI 0.519 0520), and visual disability (OR= 0.74 CI 0.736 0.738). Person with mental retardation are two times more likely to hold the disability certificate (OR 2.05 CI 2.046 2.047) than the visual disabled. States variation in percent share of disability certificate is found to be large. The result depicts that there remains a high variation in the access of disability certificates across states in India. Conclusion: A nationwide drive to include the unidentified disabled is recommended with documentation and inclusion in the administrative processes.
Comprehensive Analysis of Prognostic Significance and Immune Infiltration for FAM83 Family in Cervical Cancer
Authors: Qi Yue , Wang Yue 
Published: August 2021

Abstract
Background: This study aimed to explore the expression of Family with sequence similarity 83 (FAM83) members in cervical cancer, its prognostic value, related signaling pathways, regulatory mechanisms, and immune infiltration. It’s of great value to explore the potential role of FAM83 family in cervical cancer and provide a new scientific basis for targeted therapy.Methods: The expression, gene mutations and prognostic value of FAM83 family members in cervical cancer were analyzed by various bioinformatics tools and databases. We further explored the interaction regulation network and immune infiltration between FAM83 family members and their closely related genes through a series of databases. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes pathways (KEGG) enrichment were also conducted.Results: This study showed that the expression levels of FAM83A/B/C/D/E/F/G/H gene were upregulated in cervical cancer, the expression of FAM83B/C/D/E/F/G/H were related to tumor stages of cervical cancer, and the promoter methylation of FAM83A/D/E/F/G genes in cervical cancer were lower than those in normal tissues. What’s more, high expression of FAM83A, FAM83B, and FAM83H mRNA was associated with shortened overall survival. GO results showed that FAM83A, FAM83B, and FAM83H and their closely related genes can play an important role in cell-cell junction, calcium-dependent protein binding, regulation of peptidase activity, inflammatory response. KEGG analysis results showed that FAM83A, FAM83B, and FAM83H and their closely related genes were significantly enriched in cancer pathways, estrogen signaling pathway, MAPK signaling pathway. Furthermore, FAM83A, FAM83B, and FAM83H are all closely related to lymphocytes (Tcm_CD4, Tcm_CD8, and neutrophils) and immunomodulators (TGFBR1, TGFB1, and TNFSF9).Conclusions: With multiple databases, we found that the high expression of FAM83A, FAM83B, and FAM83H were associated with the shortened survival time and poor prognosis in patients with cervical cancer, and also closely correlated with lymphocytes and immune infiltration, suggesting that FAM83A, FAM83B, and FAM83H played an important role in the occurrence, development, malignant biological behavior, and immune infilatration of cervical cancer, which provides an important theoretical basis for early diagnosis and targeted therapy for cervical cancer.
Red Blood Cells Pathology in Patients with Coronavirus Disease 2019 (COVID-19)
Authors: Karalyan Zaven , Hakobyan Lina , Abroyan Liana , Avetisyan Aida , Avagyan Hranush , Hakobyan Sona , Bayramyan Nane , Voskanyan Henrik , Niazyan Lyudmila , Davidyants Mher , Sargsyan Knarik , Ghalechyan Tehmine , Semerjyan Anna , Karalova Elena 
Published: August 2021

Abstract
Introduction The severe acute respiratory syndrome-related coronavirus 2 (SARS-Cov-2) infection may cause hematological disorders. This pathology has been studied to a lesser extent compared to the respiratory system pathology. The main purpose of this article is to investigate the general pathology of red blood cells in coronavirus infection.MethodsIn current study 74 patients suffering from SARS-Cov-2 treated at the National Center of Infectious Diseases, Ministry of Health Armenia were included. Investigated patients were classified as: 1. Regular group (fever, respiratory symptoms and radiographic evidence of pneumonia). 2. Severe group – patients manifesting shortness of breath (over 30 breaths per minute); peripheral blood oxygen saturation below 92% in rest; pneumonia affecting over 50% of tissue; and/or respiratory failure and indicated mechanical ventilation support and/or organ failure requiring intensive care unit treatment. 3. Low saturation group with peripheral blood oxygen saturation below 85% in rest. 4. Erythroblastosis group with count of erythroblasts over 0.5% among total of nucleated blood cells. Clinical laboratory investigation included all main routine studies.Microspectrophotometry was performed on SMP-05 Opton scanning microspectrophotometer to measure the spectra of hemoglobin (Hb) in unstained erythrocytes. ResultsErythroblasts were common (present in about 30%) finding among SARS-Cov-2 patients but predominating mainly in the severe group. Serum ferritin and C-reactive protein (CRP) levels as well as anisocytosis highly correlate with the severity of the disease. Microspectrophotometric studies showed significant changes of hemoglobin adsorption spectra in individual erythrocytes. However, in severe form of SARS-CoV-2, increase of hemoglobin absorbance occurred in within 420 nm wavelength spectrum compared with regular group.ConclusionOur findings suggest that the increased levels of ferritin, CRP, anisocytosis and partially increase in Hb adsorption on 420 nm wavelength may positively correlate with adverse outcomes in SARS-Cov-2 infection.
Autism and Inner Speech: A Computational Model of Language Functions in Autistic Flexible Behaviour
Authors: Granato Giovanni , Borghi Anna M. , Mattera Andrea , Baldassarre Gianluca 
Published: August 2021

Abstract
Experimental and computational studies propose that inner speech boosts categorisation skills and executive functions, making human behaviour more focused and flexible. In addition, many clinical studies highlight a relationship between poor inner-speech and an executive impairment in autism spectrum condition (ASC), but contrasting findings are reported. Here we investigate the latter issue through a previously implemented and validated computational model of the Wisconsin Cards Sorting Tests.In particular, the model was applied to detect the individual differences in cognitive flexibility and inner speech contribution in ASC and neurotypical participants. Our results suggest that the use of inner-speech increases along the life-span of neurotypical participants but is absent in ASC ones. Although we found more attentional failures in autistic children/teenagers and more perseverative behaviours in autistic young/older adults, only ASC children and ASC older adults exhibited a lower performance than matched control groups. Overall, our results corroborate the idea that the lower use of inner speech in ASC teenagers and young adults is compensated by alternative cognitive strategies (e.g., visual thinking), but it could represent a disadvantage for children (for the missing support of development) and older adults (for the missing compensation of cognitive decline). Moreover, the results suggest that cognitive-behavioural therapies should focus on developing inner speech skills in ASC children as this could provide cognitive support along their whole life span.
Characteristics and Recovery Methods of Studies Falsely Excluded During Literature Screening –A Systematic Review
Authors: Affengruber Lisa , Dobrescu Andreea , Persad Emma , Klerings Irma , Wagner Gernot , Sommer Isolde , Gartlehner Gerald 
Published: August 2021

Abstract
Background: Due to the growing need to provide evidence syntheses under time constraints, researchers have begun focusing on the exploration of rapid review methods, which often employ single-reviewer literature screening. However, single-reviewer screening misses, on average, 13% of relevant studies, compared to 3% with dual-reviewer screening. Little guidance exists regarding methods to recover studies falsely excluded during literature screening. Likewise, it is unclear whether specific study characteristics can predict an increased risk of false exclusion. This systematic review aimed to identify supplementary search methods that can be used to recover studies falsely excluded during literature screening. Moreover, it strove to identify study-level predictors that indicate an elevated risk of false exclusions of studies during literature screening. Methods: We performed literature searches for eligible studies in Medline, Science Citation Index Expanded, Social Sciences Citation Index, Current Contents Connect, Embase, Epistemonikos.org, and Information Science & Technology Abstracts from 1999 to June 23, 2020. We searched for grey literature, checked reference lists, and conducted hand searches in two relevant journals and similar articles searches current to January 28, 2021. Two investigators independently screened the literature; one investigator performed the data extraction, a second investigator checked for correctness and completeness. Two reviewers assessed the risk of bias of eligible studies. We synthesized the results narratively. Results: Three methods studies, two with a case study design and one with a case series design, met the inclusion criteria. One study reported that all falsely excluded publications (8%) could be recovered through reference list checking compared to other supplementary search methods. No included methods study analyzed the impact of recovered studies on conclusions or meta-analyses. Two studies reported that up to 8% of studies were falsely excluded due to uninformative titles and abstracts, and one study showed that 11% of non-English studies were falsely excluded. Conclusions: Due to the limited evidence based on two case studies and one case series, we can draw no firm conclusion about the most reliable and most valid method to recover studies falsely excluded during literature screening or about the characteristics that might predict a higher risk of false exclusion. Systematic review registration: https://osf.io/v2pjr/
AHSA1 is a Prognostic Biomarker that Correlates with Macrophage Infiltration in Pan-Cancer
Authors: Li Fulei , Liu Chengdong , Chen Yanling , Li Shasha , Bai Lu 
Published: August 2021

Abstract
Background: Activator of heat shock 90 kDa protein ATPase homolog 1 (AHSA1) is differentially expressed in several tumor types. However, its association with immune cell infiltration remains elusive. Methods: AHSA1 expression was analyzed using The Cancer Genome Atlas (TCGA) pan-cancer data and normal tissue expression data from Genotype-Tissue Expression (GTEx). The clinical prognostic role of AHSA1 in pan-cancer was investigated, and an enrichment analysis of AHSA1 was performed using the R package “clusterProfiler.” We downloaded data regarding the immune cell infiltration level of TCGA pan-cancer tissues and analyzed the association between immune cell infiltration and AHSA1 expression. Results: The results of TCGA pan-cancer data analysis revealed that AHSA1 was overexpressed and associated with poor survival in patients with cancer. Furthermore, the infiltration levels of tumor-associated macrophages (TAMs) were higher, while those of CD8+ T cells were lower, in the high AHSA1 expression group. Conclusions: Our study suggests that AHSA1 is an oncogene and a risk factor for patient survival in cancer. AHSA1 may contribute to high infiltration levels of TAMs and low infiltration levels of CD8+ T cells, thus indicating that high AHSA1 expression may be associated with the tumor immunosuppressive microenvironment.
Biological Nitrogen Fixation, Carbon assimilation and plant performance of Lotus tenuis, contribute to define a strategic role in the lowlands in the Salado River Basin (Argentina)
Authors: CAMPESTRE MARIA PAULA , ANTONELLI CRISTIAN JAVIER , BAILLERES MATIAS ANDRES , MAGUIRE VANINA GISELLE , TABOADA MIGUEL ANGEL , RUIZ OSCAR ADOLFO 
Published: August 2021

Abstract
Background and AimsForage production constitutes a great challenge for the Salado River Basin (Argentina), the major area devoted to livestock in the country. The successful naturalization of the European legume Lotus tenuis has been a productive and environmental relevance for the region. This study aims to evaluate its strategic contribution, reporting for the first time the B value for this species in these marginal environments for agriculture.MethodsThe 15N natural abundance method was used to evaluate the BNF of L. tenuis at soil plots and non-leguminous weed species in the same plots were utilized as reference plants. The assays included determinations of the 13C isotope, as well. Simultaneously, evaluations were carried out in the greenhouse for the determination of the B value of L. tenuis and the relative reproducibility of the field experiments.ResultsThe results obtained demonstrated that the L. tenuis promotion is accompanied by an increase in forage quality, due to the predominance of this C3 legume species, over C4 species. Moreover, its contribution to N inputs to the system, through the BNF with native rhizobia demonstrated to be about 80%, a highly relevant percentage for these constrained agroecosystems.ConclusionsThis work supports the hypothesis that L. tenuis promotion plays a strategic role in the sustainability of the ecosystem, especially in soil constrained areas. Joined with data previously published, the results obtained contribute to support the criteria that define the naturalized legume playing a strategic role in the sustainability of agriculture marginal soils.