bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comprehensive analysis of lncRNA and mRNA based on expression microarray profiling reveals different characteristics of osteoarthritis between Tibetan and Han patients
Authors: Luo Junming , Luo Xiaoqin , Duan Zhili , Bai Wenbin , Che Xiaoming , Shan Zhongshu , Li Xiaona , Peng Jinwu 
Published: February 2021

Abstract
Background
Osteoarthritis (OA) is thought to be the most prevalent chronic joint disease, especially in Tibet of China. Here, we aimed to explore the integrative lncRNA and mRNA landscape between the OA patients of Tibet and Han.

Methods
The lncRNA and mRNA expression microarray profiling was performed by SurePrint G3 Human Gene Expression 8x60K v2 Microarray in articular cartilage samples from OA patients of Han nationality and Tibetans, followed by GO, KEGG, and trans-regulation and cis-regulation analysis of lncRNA and mRNA.

Results
We found a total of 117 lncRNAs and 297 mRNAs differently expressed in the cartilage tissues of Tibetans (n = 5) comparing with those of Chinese Han (n = 3), in which 49 lncRNAs and 158 mRNAs were upregulated, and 68 lncRNAs and 139 mRNAs were downregulated. GO and KEGG analysis showed that several unreported biological processes and signaling pathways were particularly identified. LncRNA-mRNA co-expression analysis revealed a remarkable lncRNA-mRNA relationship, in which OTOA may play a critical role in the different mechanisms of the OA progression between Tibetans and Chinese Han.

Conclusion
This study identified different lncRNA/mRNA expression profiling between OA patients of Tibetans and Han, which were involved in many characteristic biological processes and signaling pathways.
Impact of pediatric hypophosphatasia on behavioral health and quality of life
Authors: Pierpont Elizabeth I. , Simmons Jill H. , Spurlock Katherine J. , Shanley Ryan , Sarafoglou Kyriakie M. 
Published: February 2021

Abstract
Background
Hypophosphatasia (HPP) is a rare genetic disorder caused by loss-of-function mutations in the ALPL gene encoding tissue nonspecific alkaline phosphatase. It is characterized by defective bone mineralization associated with low alkaline phosphatase activity. Clinical features of pediatric HPP are highly variable, and can include premature loss of teeth, musculoskeletal problems, and impaired mobility. The effects of pediatric HPP on sleep, mood, regulation of attention and behavior, and other aspects of behavioral health have not been comprehensively studied.

Methods
Parents of 30 children with HPP (14 females, 16 males) between the ages of 3 and 16 years (mean age = 8.0 years) enrolled in this cross-sectional survey-based study. Molecular genetic and biochemical testing as well as clinical records were reviewed to verify diagnosis of HPP. The cohort included 15 patients with a more clinically severe presentation of HPP who had received treatment with enzyme replacement therapy (asfotase alfa) and 15 children with less severe HPP who were treatment-naïve. Parents provided information regarding psychopathological comorbidity, emotional and behavioral well-being, and quality of life.

Results
Clinically significant behavioral health challenges were evident in 67% of children with HPP. The most common behavioral findings included sleep disturbance and symptoms of attention deficit hyperactivity disorder (ADHD), each of which were observed ≥ 50% of individuals. Sleep disturbance, pain interference, poor behavioral regulation, and mood/anxiety symptoms were associated with reduced physical and psychosocial quality of life. Behavioral concerns were evident among children with HPP receiving asfotase alfa treatment as well as among children with clinically less severe disease who had not initiated therapy. Although most children in the cohort (77%) had age-typical development of adaptive skills, emotional and behavioral challenges were associated with weaker adaptive function.

Conclusions
Children with HPP are at increased risk for ADHD symptoms and other behavioral health challenges. There is likely an under-recognition of these findings in clinical practice.
Selenium levels and glutathione peroxidase activity in patients with ataxia-telangiectasia: association with oxidative stress and lipid status biomarkers
Authors: Andrade Itana Gomes Alves , Suano-Souza Fabíola Isabel , Fonseca Fernando Luiz Affonso , Lago Carolina Sanchez Aranda , Sarni Roseli Oselka Saccardo 
Published: February 2021

Abstract
Introduction
Ataxia-Telangiectasia (A-T) is a multi-system disorder that may be associated with endocrine changes, oxidative stress in addition to inflammation. Studies suggest that selenium is a trace element related to protection against damage caused by oxidative stress.

Objective
To describe the plasma levels of selenium and erythrocyte glutathione peroxidase activity in A-T patients and to relate them to oxidative stress and lipid status biomarkers.

Methods
This is a cross-sectional and controlled study evaluating 22 A-T patients (age median, 12.2 years old) matched by gender and age with 18 healthy controls. We evaluated: nutritional status, food intake, plasma selenium levels, erythrocyte glutathione peroxidase activity, lipid status, inflammation and oxidative stress biomarkers.

Results
Adequate levels of selenium were observed in 24/36 (66.7%) in this evaluated population. There was no statistically significant difference between the groups in selenium levels [47.6 μg/L (43.2–57.0) vs 54.6 (45.2–62.6) μg/dL, p = 0.242]. Nine of A-T patients (41%) had selenium levels below the reference value. The A-T group presented higher levels of LDL-c, non-HDL-c, oxidized LDL, Apo B, Apo-B/Apo-A-I1, LDL-c/HDL-c ratio, malondialdehyde [3.8 µg/L vs 2.8 µg/L, p = 0.029] and lower Apo-A-I1/HDL-c and glutathione peroxidase activity [7300 U/L vs 8686 U/L, p = 0.005]. Selenium levels were influenced, in both groups, independently, by the concentrations of oxidized LDL, malonaldehyde and non-HDL-c. The oxidized LDL (AUC = 0.849) and ALT (AUC = 0.854) were the variables that showed the greatest discriminatory power between groups.

Conclusion
In conclusion, we observed the presence of selenium below the reference value in nearly 40% and low GPx activity in A-T patients. There was a significant, inverse and independent association between selenium concentrations and oxidative stress biomarkers. Those data reinforce the importance of assessing the nutritional status of selenium in those patients.
Comparative study of the effect of neuromuscular electrical stimulation and oral administration of branched-chain amino acid on preventing sarcopenia in patients after living-donor liver transplantation: study protocol for an open-label randomized controlled trial
Authors: Haraguchi Masafumi , Ichinose Kunihiro , Miyaaki Hisamitsu , Hanada Masatoshi , Fukushima Masanori , Sasaki Ryu , Miuma Satoshi , Hara Takanobu , Kugiyama Tota , Soyama Akihiko , Hidaka Masaaki , Tsuji Ayumi , Yano Rintaro , Sekino Motohiro , Takahata Hideaki , Eguchi Susumu , Nakao Kazuhiko 
Published: February 2021

Abstract
Background
Liver cirrhosis is the irreversible fibrosis of the liver and causes refractory ascites and hepatic encephalopathy, which might not respond to treatment. Living donor liver transplantation (LDLT) is an effective treatment for patients with cirrhosis. However, post-LDLT patients are prone to muscle atrophy and sarcopenia. Therefore, physiotherapy of post-LDLT patients is essential for preventing the progression of sarcopenia. Recently, rehabilitation using neuromuscular electrical stimulation (NMES) has been reported to be useful for preventing the progression of sarcopenia. Similarly, nutrition therapy is essential for post-LDLT patients because these patients frequently experience malnutrition. However, the effects of combined NMES and nutrition therapy on post-LDLT patients remain unknown.

Methods/design
This open-label, randomized, parallel-group study will compare the effects of combined therapy with NMES and branched-chain amino acids (BCAA) with those of NMES alone in patients with decompensated cirrhosis after LDLT. After LDLT, 50 patients with decompensated cirrhosis will be randomly assigned to receive NMES with BCAA or NMES without BCAA. The duration of the intervention will be 3 months. To analyze the change in skeletal muscle mass, InBody 770 body composition and body water analysis and ultrasonography will be performed before LDLT and 4 weeks and 12 weeks post-LDLT. The primary endpoint is changes in the skeletal muscle mass from baseline to 3 months. Important secondary endpoints are the changes in the skeletal muscle mass from baseline to 1 month and changes in the quadriceps strength from baseline to 1 month.

Discussion
The results of this study are expected to provide evidence regarding the effect of NMES combined with BCAA therapy on the skeletal muscle of post-LDLT patients.

Trial registration
Japan Registry of Clinical Research jRCTs071190051. Registered on February 26, 2020.
Targeted deletion of Ruvbl1 results in severe defects of epidermal development and perinatal mortality
Authors: Dafinger Claudia , Benzing Thomas , Dötsch Jörg , Schermer Bernhard , Liebau Max C. 
Published: February 2021

AbstractEpidermal development is a complex process of regulated cellular proliferation, differentiation, and tightly controlled cell death involving multiple cellular signaling networks. Here, we report a first description linking the AAA+ (ATPases associated with various cellular activities) superfamily protein Ruvbl1 to mammalian epidermal development. Keratinocyte-specificRuvbl1knockout mice (Ruvbl1fl/flK14:Cretg) show a severe phenotype including dramatic structural epidermal defects resulting in the loss of the functional skin barrier and perinatal death. Thus,Ruvbl1is a newly identified essential player for the development of differentiated epidermis in mice.
Left open spaces—light shafts in Iran and side setbacks in Japan: a socio-spatial approach to study awareness in open spaces in urban residential blocks
Authors: Shojai Amir , Fattahi Kaveh 
Published: February 2021

AbstractThis article addresses an urban design issue at a micro-urban design level; the spaces between buildings on neighboring blocks or the side yards, how such spaces they are used, and their social impact on residents. A socio-psychological approach was taken into studying arrangements of these open spaces in contemporary residential zones in—side setback spaces in Japan, and backyards and light shafts in Iran in terms of their similarities and differences. The research involves development policies and interviews with residents in buildings where such conditions prevail, in two different contexts—Shiraz, Iran and Sapporo, Japan, two cities comparative in their size and densities for their cultural differences might have on their social responses. Seventy-two respondents in Shiraz District 6 were recruited by Shiraz University architecture students and in Japan, 75 responses were collected in Central Ward, Sapporo, from various groups of residents. Majority of the windows to side setbacks in Sapporo had matte glasses; therefore, privacy concerns were not among residents. Lack of maintenance in inner courtyards was a major concern for Iranians and they see side setbacks as a challenge to their and safety, however providing opportunities for neighborly atmosphere, if the windows have matte glasses and provided with guards. It argues that contacts are not controlled are perceived the same in two different cultures; however, the arrangement of open spaces play a role in light reception and ventilation in Japanese example.
Can a penalized-likelihood estimation algorithm be used to reduce the injected dose or the acquisition time in 68Ga-DOTATATE PET/CT studies?
Authors: Chicheportiche Alexandre , Goshen Elinor , Godefroy Jeremy , Grozinsky-Glasberg Simona , Oleinikov Kira , Meirovitz Amichay , Gross David J. , Ben-Haim Simona 
Published: February 2021

Abstract
Background
Image quality and quantitative accuracy of positron emission tomography (PET) depend on several factors such as uptake time, scanner characteristics and image reconstruction methods. Ordered subset expectation maximization (OSEM) is considered the gold standard for image reconstruction. Penalized-likelihood estimation (PL) algorithms have been recently developed for PET reconstruction to improve quantitation accuracy while maintaining or even improving image quality. In PL algorithms, a regularization parameter β controls the penalization of relative differences between neighboring pixels and determines image characteristics. In the present study, we aim to compare the performance of Q.Clear (PL algorithm, GE Healthcare) and OSEM (3 iterations, 8 subsets, 6-mm post-processing filter) for 68Ga-DOTATATE (68Ga-DOTA) PET studies, both visually and quantitatively.
Thirty consecutive whole-body 68Ga-DOTA studies were included. The data were acquired in list mode and were reconstructed using 3D OSEM and Q.Clear with various values of β and various acquisition times per bed position (bp), thus generating images with reduced injected dose (1.5 min/bp: β = 300–1100; 1.0 min/bp: β = 600–1400 and 0.5 min/bp: β = 800–2200). An additional analysis adding β values up to 1500, 1700 and 3000 for 1.5, 1.0 and 0.5 min/bp, respectively, was performed for a random sample of 8 studies. Evaluation was performed using a phantom and clinical data. Two experienced nuclear medicine physicians blinded to the variables assessed the image quality visually.

Results
Clinical images reconstructed with Q.Clear, set at 1.5, 1.0 and 0.5 min/bp using β = 1100, 1300 and 3000, respectively, resulted in images with noise equivalence to 3D OSEM (1.5 min/bp) with a mean increase in SUVmax of 14%, 13% and 4%, an increase in SNR of 30%, 24% and 10%, and an increase in SBR of 13%, 13% and 2%. Visual assessment yielded similar results for β values of 1100–1400 and 1300–1600 for 1.5 and 1.0 min/bp, respectively, although for 0.5 min/bp there was no significant improvement compared to OSEM.

Conclusion
68Ga-DOTA reconstructions with Q.Clear, 1.5 and 1.0 min/bp, resulted in increased tumor SUVmax and in improved SNR and SBR at a similar level of noise compared to 3D OSEM. Q.Clear with β = 1300–1600 enables one-third reduction of acquisition time or injected dose, with similar image quality compared to 3D OSEM.
A parametric study on cyclic strength of coastal sand of Digha in West Bengal, India
Authors: Ray Pinak , Sahu Ramendu Bikas 
Published: February 2021

AbstractStress controlled cyclic triaxial tests have been carried out on coastal sand of Digha, West Bengal, India at different frequencies, confining pressures, relative densities and number of loading cycles for determination of influence of these parameters on cyclic strength (expressed in terms of cyclic stress ratio) and initial liquefaction of Digha sand. The test results provide evidence that increasing density of sand increases liquefaction potential, though it has been found that increase in effective confining pressure reduces cyclic strength of sand. Cyclic strength of sand decreases with increase of number of loading cycles at a specific density and a particular confining pressure. It has been observed that frequency of loading cycles does have any significant influence on the number of cycles for initial liquefaction of Digha sand. An empirical correlation has been developed on cyclic strength of sand based on these parameters and it has been found that this correlation fits quite well with the observed experimental results.
Costly Interpretation of Asset Prices
Authors: Mondria Jordi , Vives Xavier , Yang Liyan 
Published: February 2021

We propose a model in which investors cannot costlessly process information from asset prices. At the trading stage, investors are boundedly rational, and their interpretation of prices injects noise into the price, generating a source of endogenous noise trading. Our setup predicts price momentum and yields excessive return volatility and excessive trading volume. In an overall equilibrium, investors optimally choose sophistication levels by balancing the benefit of beating the market against the cost of acquiring sophistication. There can exist strategic complementarity in sophistication acquisition, leading to multiple equilibria. This paper was accepted by Gustavo Manso, finance.
<p><strong>An updated checklist of Turkish crabronid wasps (Hymenoptera: Crabronidae) with new and additional records</strong></p>
Authors: KAPLAN EMIN , YILDIRIM EROL 
Published: February 2021

An updated checklist of the wasp family Crabronidae in Turkey is provided. It is based on records present in the literature and on newly identified specimens collected in Turkey. In this study, 537 species and 16 subspecies in 65 genera are listed. Among them, five species, namely Dryudella esterinae Pagliano, 2001, Nysson mimulus Valkeila, 1964, Crossocerus pullulus A. Morawitz, 1866, Oxybelus spectabilis Gerstaecker, 1867 and Diodontus insidiosus Spooner, 1938 are recorded for the first time from Turkey. New and additional records for 114 species are given. Collection localities and photographs of newly identified species are provided. Key words: Hymenoptera, Crabronidae, checklist, new records, additional records, Turkey
Adaptation and performance of a mobile application for early detection of cutaneous leishmaniasis
Authors: Rubiano Luisa , Alexander Neal D. E. , Castillo Ruth Mabel , Martínez Álvaro José , García Luna Jonny Alejandro , Arango Juan David , Vargas Leonardo , Madriñán Patricia , Hurtado Lina-Rocío , Orobio Yenifer , Rojas Carlos A. , del Corral Helena , Navarro Andrés , Gore Saravia Nancy , Aronoff-Spencer Eliah 
Published: February 2021

Background
Detection and management of neglected tropical diseases such as cutaneous leishmaniasis present unmet challenges stemming from their prevalence in remote, rural, resource constrained areas having limited access to health services. These challenges are frequently compounded by armed conflict or illicit extractive industries. The use of mobile health technologies has shown promise in such settings, yet data on outcomes in the field remain scarce.

Methods
We adapted a validated prediction rule for the presumptive diagnosis of CL to create a mobile application for use by community health volunteers. We used human-centered design practices and agile development for app iteration. We tested the application in three rural areas where cutaneous leishmaniasis is endemic and an urban setting where patients seek medical attention in the municipality of Tumaco, Colombia. The application was assessed for usability, sensitivity and inter-rater reliability (kappa) when used by community health volunteers (CHV), health workers and a general practitioner, study physician.

Results
The application was readily used and understood. Among 122 screened cases with cutaneous ulcers, sensitivity to detect parasitologically proven CL was >95%. The proportion of participants with parasitologically confirmed CL was high (88%), precluding evaluation of specificity, and driving a high level of crude agreement between the app and parasitological diagnosis. The chance-adjusted agreement (kappa) varied across the components of the risk score. Time to diagnosis was reduced significantly, from 8 to 4 weeks on average when CHV conducted active case detection using the application, compared to passive case detection by health facility-based personnel.

Conclusions
Translating a validated prediction rule to a mHealth technology has shown the potential to improve the capacity of community health workers and healthcare personnel to provide opportune care, and access to health services for underserved populations. These findings support the use of mHealth tools for NTD research and healthcare.
Knowledge, attitudes, and practices of seasonal influenza vaccination among older adults in nursing homes and daycare centers, Honduras
Authors: Madewell Zachary J. , Chacón-Fuentes Rafael , Jara Jorge , Mejía-Santos Homer , Molina Ida-Berenice , Alvis-Estrada Juan Pablo , Espinal Raul 
Published: February 2021

Background
Older adults represent 70–90% of seasonal influenza-related deaths and 50–70% of influenza-related hospitalizations. Vaccination is the most efficient means of preventing influenza and reducing influenza-related illnesses. We aimed to describe knowledge, attitudes, and practices (KAP) of seasonal influenza vaccination among older adults in Honduras.

Methods
From August 29–October 26, 2018, we conducted a cross-sectional KAP survey regarding seasonal influenza vaccinations to samples of older adults 1) admitted to nursing homes and 2) attending daycare centers. We used the Minimental State Examination (MMSE) psychometric tool to assess the cognitive status of older adults and included participants with scores of ≥23 points in the survey. We reported frequency distributions for demographics, KAP of influenza virus and vaccination, and vaccination coverage. We used logistic regression to analyze associations between demographics and verified influenza vaccination.

Results
Of 511 MMSE participants, 341 completed the survey (95 adults in 12 nursing homes and 246 older adults in ten daycare centers). Almost all participants knew that influenza causes severe illness and may be transmitted from person to person, vaccination is safe and protects against disease, and older adults have greater risk of complications. Of 284 participants with verified vaccinations, 81.3% were vaccinated for influenza: 87.9% attending daycare centers and 61.4% in nursing homes. Among all participants, verified current influenza vaccination was associated with self-reported influenza vaccination in previous year (aOR: 14.05; 95% CI: 5.36–36.81); no formal education (aOR: 4.83; 95% CI: 1.63–14.37) or primary school education (aOR: 4.51; 95% CI: 1.79–11.37) having ≥secondary as reference; and indigenous (aOR: 4.55; 95% CI: 1.18–17.49) having Mestizo as reference. Reasons for vaccination were perceived self-benefits, protection against influenza complications, favorable vaccination hours, and healthcare provider recommendations.

Conclusion
Four-fifths of older adults were vaccinated for seasonal influenza. Educational efforts provided in conjunction with vaccination campaigns resulted in high knowledge of influenza virus, transmission, and vaccination. Further outreach regarding disease risks and vaccine safety needs to be directed towards older adults in nursing homes who had lower knowledge and coverage than older adults in daycare centers.