bluuebunny/crossref_metadata_2025_split huggingface dataset:
DDIT4 S‐Nitrosylation Aids p38‐MAPK Signaling Complex Assembly to Promote Hepatic Reactive Oxygen Species Production
Authors: Li Zilong , Zhao Qianwen , Lu Yunjie , Zhang Yangxi , Li Luyang , Li Min , Chen Xuemin , Sun Donglin , Duan Yunfei , Xu Yong 
Published: July 2021

AbstractMitogen‐activated protein kinase (MAPK) signaling plays a significant role in reactive oxygen species (ROS) production. The authors have previously shown that Brahma‐related gene 1 (BRG1), a chromatin remodeling protein, contributes to hepatic ROS accumulation in multiple animal and cellular models of liver injury. Here it is reported that DNA damage‐induced transcript 4 (DDIT4) is identified as a direct transcriptional target for BRG1. DDIT4 overexpression overcomes BRG1 deficiency to restore ROS production whereas DDIT4 knockdown phenocopies BRG1 deficiency in suppressing ROS production in vitro and in vivo. Mechanistically, DDIT4 coordinates the assembly of the p38‐MAPK signaling complex to drive ROS production in an S‐nitrosylation dependent manner. Molecular docking identifies several bioactive DDIT4‐inteacting compounds including imatinib, nilotinib, and nateglinide, all of which are confirmed to attenuate hepatic ROS production, dampen p38‐MAPK signaling, and ameliorate liver injury by influencing DDIT4 S‐nitrosylation. Importantly, positive correlation between ROS levels and BRG1/DDIT4/S‐nitrosylated DDIT4 levels is detected in human liver biopsy specimens. In conclusion, the data reveal a transcription‐based signaling cascade that contributes to ROS production in liver injury.
Highly Efficient, Surface Ligand Modified Quantum Dot Light‐Emitting Diodes Driven by Type‐Controllable MoTe2 Thin Film Transistors via Electron Charge Enhancer
Authors: Baek Geun Woo , Seo Seung Gi , Hahm Donghyo , Bae Wan Ki , Kwak Jeonghun , Jin Sung Hun 
Published: July 2021

AbstractThe development of transition metal dichalcogenides (TMDCs) and quantum dots (QDs) as the promising semiconductor and emitter is carried out in diverse applications. Despite superb research progress, study of emerging devices incorporated with TMDC thin film transistors (TFTs) and quantum dot light‐emitting diodes (QLEDs) is rarely reported. Herein, the QLEDs operation controlled by p (or n‐type) molybdenum ditelluride (MoTe2) TFTs with the realization of complementary type transistor is first demonstrated. In this study, molecular doping by poly‐L‐lysine (PLL) is adopted for a type conversion of MoTe2 TFTs and surface ligand modification is utilized for improvement of QLED performance. As a result, the PLL treatment achieves the outstanding type conversion of MoTe2 TFTs without any degradation of electrical properties, leading to securing reliable n or p‐type devices, thus, availability of complementary circuits. Furthermore, ligand modified QDs capped with octylamine result in balanced electron/hole injection in QLEDs, yielding improved current efficiency (ηA = 13.9 cd A−1) and longer lifetimes (T50 = 66 h at L0 = 3000 cd m−2). Lastly, MoTe2 TFTs demonstrate their capabilities to drive the QLEDs for the envisioned application including display backplane transistor with decent switching properties, immunity for generation of photocurrent, and operation stability.
Evaluation of interseeding cover crop mixtures in Mid‐Atlantic double‐crop soybean
Authors: Peterson Cara M. , Mirsky Steven B. , VanGessel Mark J. , Davis Brian W. , Ackroyd Victoria J. , Tully Katherine L. 
Published: July 2021

AbstractWinter legume cover crops can provide organic nitrogen (N) to the subsequent corn (Zea mays L.) crop. However, legume cover crop adoption is limited in the Mid‐Atlantic United States by the short establishment window after double‐crop soybean [Glycine max (L.) Merr.] harvest. Interseeding legume cover crops into double‐crop soybean with a modified seed drill could improve on previous interseeding methods. We conducted field trials in which four cereal rye (Secale cereale L.) + legume mixtures were interseeded into double‐crop soybean on 76‐cm row spacing. Double‐crop soybean yield was compared between the 76‐cm row spacing required for interseeding and the more common 38‐cm row spacing. Overall, there was minimal effect of row spacing or interseeding on double‐crop soybean yield. Cover crop biomass varied by species, ranging from 1 to 7 Mg ha–1. Cover crop biomass N content was higher in cereal rye + legume treatments (mean, 79 kg N ha–1) than in cereal rye alone (mean, 30 kg N ha–1; p < .001). We also examined the effect of fall‐interseeded cover crop mixtures on corn yield. Corn yields were low in 2018, yet we observed higher corn yields in the cereal rye + hairy vetch (Vicia villosa Roth) cover crop compared with no cover crop and cereal rye alone. In 2019, there was no effect of cover crops on corn yields. This study demonstrated the specific benefits of integrating legumes into cropping rotations, namely increased N cycling efficiency and resiliency to extreme weather patterns.
Farmers' attitude toward CRISPR/Cas9: The case of blast resistant rice
Authors: Ferrari Linda 
Published: July 2021

AbstractNew breeding techniques (NBTs) for genetically modified (GM) crops, such as CRISPR/Cas9, could represent a valuable solution for achieving a sustainable agricultural system. This study examines Italian farmers' attitudes toward CRISPR/Cas9‐modified blast‐resistant rice in a hypothetical context. It is one of the first studies to investigate farmers' attitudes toward NBTs. Additionally, it explores the role of sociodemographics, farm characteristics, knowledge, perceived risks, and the locus of control (LOC) in shaping attitudes toward CRISPR/Cas9‐modified rice. The results show that farmers are open to this innovation and will cultivate CRISPR/Cas9‐modified rice if it is available in the market. Moreover, we find subjective knowledge, practice experience, and low perceived risk in the agri‐food business to be positively associated with attitudes toward CRISPR/Cas9‐modified blast‐resistant rice. Notably, LOC is found to be inversely related to attitude, indicating that having an external LOC improves attitudes toward CRISPR/Cas9‐modified rice. The findings suggest that farmers are generally favorable to the introduction of CRISPR/Cas9 technology; thus, a revision of the current European Union policies on GM crops could meet their needs. [EconLit Citations: Q18, Q16].
Prognostic significance of osteopenia in patients with colorectal cancer: A retrospective cohort study
Authors: Kamada Teppei , Furukawa Kenei , Takahashi Junji , Nakashima Keigo , Nakaseko Yuichi , Suzuki Norihiko , Yoshida Masashi , Ohdaira Hironori , Ikegami Toru , Suzuki Yutaka 
Published: July 2021

AbstractAimWe examined the prognostic impact of osteopenia on the long‐term outcomes of patients with colorectal cancer after laparoscopic colectomy along with other nutritional factors, including sarcopenia or the Glasgow Prognostic Score.MethodsThis retrospective cohort study analyzed the data of 230 patients with stage Ⅰ–Ⅲ colorectal cancers who underwent surgical resection between November 2010 and December 2015. Osteopenia and sarcopenia were evaluated by measuring the average pixel density in the mid‐vertebral core of the 11th thoracic vertebra on enhanced computed tomography and the psoas muscle mass area at the third lumbar vertebra, respectively. The overall survival and disease‐free survival rates were analyzed using Cox proportional hazards model and Kaplan–Meier curves with the log‐rank test.ResultsOsteopenia was identified in 43 patients (18.7%). Univariate analysis showed that the disease‐free survival rate was significantly worse in patients with stage II–III cancers, vascular invasion, carcinoembryonic antigen (CA) >5.0 ng/mL, CA19‐9 > 37.0 U/mL, sarcopenia, and osteopenia (all P < .01). Multivariate analysis revealed that stage II–III cancers (P = .01), vascular invasion (P = .01), carcinoembryonic antigen >5.0 (P < .01), and osteopenia (P < .01) were significant independent disease‐free survival predictors. In univariate analysis, the overall survival rate significantly decreased in patients with stage II–III cancers (P = .03), carcinoembryonic antigen >5.0 (P < .01), CA19‐9 > 37.0 (P < .01), sarcopenia (P < .01), and osteopenia (P < .01). Multivariate analysis indicated that carcinoembryonic antigen >5.0 (P = .04), CA19‐9 > 37.0 (P = .05), and osteopenia (P < .01) were significant independent predictors of overall survival.ConclusionPreoperative osteopenia could be a strong predictor of long‐term outcomes in patients undergoing resection for colorectal cancer.
In vivo deciduous dental eruption in LuiKotale bonobos and Gombe chimpanzees
Authors: Lee Sean M. , Sutherland L. J. , Fruth Barbara , Murray Carson M. , Lonsdorf Elizabeth V. , Arbenz‐Smith Keely , Augusto Rafael , Brogan Sean , Canington Stephanie L. , Lee Kevin C. , McGrath Kate , McFarlin Shannon C. , Hohmann Gottfried 
Published: July 2021

AbstractObjectivesExisting data on bonobo and chimpanzee dental eruption timing are derived predominantly from captive individuals or deceased wild individuals. However, recent advances in noninvasive photographic monitoring of living, wild apes have enabled researchers to characterize dental eruption in relatively healthy individuals under naturalistic conditions. At present, such data are available for only one population of wild chimpanzees. We report data for an additional population of wild chimpanzees and the first dental eruption data for wild bonobos.Materials and MethodsWe collected photographs and video footage of teeth from the open mouths of wild bonobos and East African chimpanzees of known age from LuiKotale, Democratic Republic of the Congo, and Gombe National Park, Tanzania, respectively. We scored the presence and absence of deciduous teeth from photographs and video footage to characterize deciduous dental eruption timing in these two populations.ResultsDeciduous dental eruption ages in our sample fall within the range of variation previously documented for captive chimpanzees, but eruption ages are later in wild than in captive contexts. We found substantial variation in deciduous canine eruption timing, particularly among bonobos. One bonobo had a deciduous canine present by 227 days old while another did not have a deciduous canine present at 477 days old.DiscussionOur data indicate that deciduous teeth erupt later in wild individuals than in captive individuals. We also found that deciduous dental eruption timing varies considerably between individuals within our study populations, a pattern that is consistent with previous studies. Future studies should consider sources of variation in deciduous canine eruption timing and relationships with other aspects of life history as additional data become available.
Low‐Density 2D Superlattices Assembled via Directional DNA Bonding
Authors: Miao Ziyi , Zheng Cindy Y. , Schatz George C. , Lee Byeongdu , Mirkin Chad A. 
Published: July 2021

AbstractIt is critical to assemble nanoparticles (NPs) into superlattices with controlled symmetries and spacings on substrates for metamaterials applications, where such structural parameters dictate their properties. Here, we use DNA to assemble anisotropic NPs of three shapes—cubes, octahedra, and rhombic dodecahedra—on substrates and investigate their thermally induced reorganization into two‐dimensional (2D) crystalline films. We report two new low‐density 2D structures, including a honeycomb lattice based on octahedral NPs. The low‐density lattices favored here are not usually seen when particles are crystallized via other bottom‐up assembly techniques. Furthermore, we show that, consistent with the complementary contact model, a primary driving force for crystallization is the formation of directional, face‐to‐face DNA bonds between neighboring NPs and between NPs and the substrate. Our results can be used to deliberately prepare crystalline NP films with novel morphologies.
The Effect of Weak Adsorption on the Double Layer Capacitance
Authors: Schmickler Wolfgang 
Published: July 2021

AbstractMotivated by recents results for the double‐layer on Pt(111), I reexamine the theory of weak adsorption on double‐layer properties, and use it to explore the capacitance at low electrolyte concentrations. Even a very weak adsorption enhances the double layer capacitance, so that it may exceed the value CGC predicted by Gouy‐Chapman theory. A Parsons‐Zobel plot of
vs.
for the experimental capacity C does not result in a straight line of unit slope, but roughly follows a linear relation with smaller slope. These results are in line with older data on Ag(111), and with the new data on Pt(111).
Structure and Functional Differences of Cysteine and 3‐Mercaptopropionate Dioxygenases: A Computational Study
Authors: Yeh C.‐C. George , Pierides Christos , Jameson Guy N. L. , de Visser Sam P. 
Published: July 2021

AbstractThiol dioxygenases are important enzymes for human health; they are involved in the detoxification and catabolism of toxic thiol‐containing natural products such as cysteine. As such, these enzymes have relevance to the development of Alzheimer's and Parkinson's diseases in the brain. Recent crystal structure coordinates of cysteine and 3‐mercaptopropionate dioxygenase (CDO and MDO) showed major differences in the second‐coordination spheres of the two enzymes. To understand the difference in activity between these two analogous enzymes, we created large, active‐site cluster models. We show that CDO and MDO have different iron(III)‐superoxo‐bound structures due to differences in ligand coordination. Furthermore, our studies show that the differences in the second‐coordination sphere and particularly the position of a positively charged Arg residue results in changes in substrate positioning, mobility and enzymatic turnover. Furthermore, the substrate scope of MDO is explored with cysteinate and 2‐mercaptosuccinic acid and their reactivity is predicted.
Inhibitors of Human Divalent Metal Transporters DMT1 (SLC11A2) and ZIP8 (SLC39A8) from a GDB‐17 Fragment Library
Authors: Pujol‐Giménez Jonai , Poirier Marion , Bühlmann Sven , Schuppisser Céline , Bhardwaj Rajesh , Awale Mahendra , Visini Ricardo , Javor Sacha , Hediger Matthias A. , Reymond Jean‐Louis 
Published: July 2021

AbstractSolute carrier proteins (SLCs) are membrane proteins controlling fluxes across biological membranes and represent an emerging class of drug targets. Here we searched for inhibitors of divalent metal transporters in a library of 1,676 commercially available 3D‐shaped fragment‐like molecules from the generated database GDB‐17, which lists all possible organic molecules up to 17 atoms of C, N, O, S and halogen following simple criteria for chemical stability and synthetic feasibility. While screening against DMT1 (SLC11A2), an iron transporter associated with hemochromatosis and for which only very few inhibitors are known, only yielded two weak inhibitors, our approach led to the discovery of the first inhibitor of ZIP8 (SLC39A8), a zinc transporter associated with manganese homeostasis and osteoarthritis but with no previously reported pharmacology, demonstrating that this target is druggable.
Population Pharmacokinetic Model Development and Simulation for Recombinant Erwinia Asparaginase Produced in Pseudomonas fluorescens (JZP‐458)
Authors: Lin Tong , Dumas Todd , Kaullen Josh , Berry N. Seth , Choi Mi Rim , Zomorodi Katie , Silverman Jeffrey A. 
Published: July 2021

AbstractJZP‐458 is a recombinant Erwinia asparaginase produced using a novel Pseudomonas fluorescens expression platform that yields an enzyme expected to lack immunologic cross‐reactivity to Escherichia coli–derived asparaginases. It is being developed as part of a multiagent chemotherapeutic regimen to treat acute lymphoblastic leukemia or lymphoblastic lymphoma patients who develop E coli–derived asparaginase hypersensitivity. A population pharmacokinetic (PopPK) model was developed for JZP‐458 using serum asparaginase activity (SAA) data from a phase 1, single‐dose study (JZP458‐101) in healthy adults. Effects of intrinsic covariates (body weight, body surface area, age, sex, and race) on JZP‐458 PK were evaluated. The model included SAA data from 24 healthy adult participants from the phase 1 study who received JZP‐458: intramuscular (IM) data at 12.5 mg/m2 (N = 6) and 25 mg/m2 (N = 6), and intravenous (IV) data at 25 mg/m2 (N = 6) and 37.5 mg/m2 (N = 6). Model simulations of adult and pediatric SAA profiles were performed to explore the likelihood of achieving a therapeutic target nadir SAA (NSAA) level ≥0.1 IU/mL based on different administration strategies. PopPK modeling and simulation suggest JZP‐458 is expected to achieve 72‐hour NSAA levels ≥0.1 IU/mL in 100% of adult or pediatric populations receiving IM administration at 25 mg/m2, and in 80.9% of adult and 94.5% of pediatric populations receiving IV administration at 37.5 mg/m2 on a Monday/Wednesday/Friday (M/W/F) dosing schedule. Based on these results, the recommended starting dose for the phase 2/3 pivotal study is 25 mg/m2 IM or 37.5 mg/m2 IV on a M/W/F dosing schedule in pediatric and adult patients.
A scalable platform for big data analysis in public transport
Authors: Uçak Ege , Karagümüş Elif , Şener Cevat 
Published: July 2021

SummaryAny life event or action can be seen as a potential source of data to analyze. By analyzing such data, we can gain insights into the facts. The situation is no different in public transport. Researchers working in the fields of transport and traffic have stated that such an analysis would be invaluable in designing urban transport and particularly in adapting to current changes. In this study, a scalable public transport analysis platform named Cermoni is developed using the Apache Beam programming model. It can analyze in near‐real‐time smart card and vehicle location data collected, classified as big data with its high production speed. The performance of the platform was tested on Google Cloud Dataflow service using real‐world data gathered from Konya, one of the largest metropolitan cities in Turkey, and the results are discussed in detail.
Radical‐Mediated Acyl Thiol‐Ene Reaction for Rapid Synthesis of Biomolecular Thioester Derivatives
Authors: McLean Joshua T. , Milbeo Pierre , Lynch Dylan M. , McSweeney Lauren , Scanlan Eoin M. 
Published: July 2021

AbstractThe thiol‐ene ‘click’ reaction has emerged as a versatile process for carbon–sulfur bond formation with widespread applications in chemical biology, medicinal chemistry and materials science. Thioesters are key intermediates in a wide range of synthetic and biological processes and efficient methods for their synthesis are of considerable interest. Herein, we report the first examples of acyl‐thiol‐ene (ATE) for the synthesis of biomolecular thioesters, including peptide, lipid and carbohydrate derivatives. A key finding is the profound effect of the amino acid side chain on the outcome of the ATE reaction. Furthermore, radical generated thioesters underwent efficient S‐to‐N acyl transfer and desulfurisation to furnish ‘sulfur‐free’ ligation products in an overall amidation process with diverse applications for chemical ligation and bioconjugation.
A unique epithelioid vascular neoplasm of bone characterized by EWSR1/FUS‐NFATC1/2 fusions
Authors: Dashti Nooshin K. , Dickson Brendan C. , Zhang Lei , Xie Ziyu , Nielsen Gunnlaugur Pétur , Antonescu Cristina R. 
Published: July 2021

AbstractAn increasing number of epithelioid vascular lesions, in particular tumors from the benign and low‐grade end of the spectrum, have been characterized by recurrent gene fusions. As a result, the detection of these molecular markers have improved the classification of diagnostically challenging cases. However, despite the significant progress, there are occasional lesions that do not fit in known histologic or molecular groups. Herein, we present five such unclassified epithelioid vascular lesions, which occurred in the bone and showed a distinct morphology composed of alternating vasoformative and solid growth and mild to moderate nuclear pleomorphism. The variegated morphologic appearance resembled that of composite hemangioendothelioma, being distinct from both epithelioid hemangioma and epithelioid hemangioendothelioma, and consistently showed cytologic atypia. Due to their unusual morphologic appearance and negative molecular work‐up, targeted transcriptome sequencing was performed in two cases showing the presence of NFATC2 fusions with either EWSR1 or FUS genes. Three additional bone tumors with EWSR1 gene rearrangements were identified by FISH screening of a large cohort of 45 fusion‐negative epithelioid vascular neoplasms, one fused to NFATC2 while two others to NFATC1. There were three females and two males, with a wide age range at presentation, mean of 44 years. The lesions occurred in the pelvis, maxillary sinus, and humerus. Two patients presented with polyostotic disease, both located in the pelvic bones. Two patients had available follow‐up, one developed two local recurrences in the humerus over a 15‐year period, while the other showed no recurrence 4 years subsequent to an en‐bloc resection. Tumors were positive for CD31 and ERG, while negative for EMA, CK, synaptophysin, and chromogranin. FISH confirmed this abnormality in all cases, none of them being associated with gene amplifications. Further studies are needed to establish the pathogenetic relationship of this rare molecular subset with other epithelioid vascular tumors and to determine its clinical behavior.
RBCK1‐TRIB3 decelerated the progression of acute promyelocytic leukemia
Authors: Zhang Xiang , Yu Wenjuan 
Published: July 2021

AbstractUnder the differentiation induction therapy with all‐trans retinoic acid and arsenic trioxide, nearly 95% of typical acute promyelocyte leukemia (APL), which is characterized by the presence of PML‐RARA, patients can be cured. Though its good prognosis, if left untreated, the natural survival duration of typical APL patients is only 1 month, but some exceptional cases also exist. Occasionally, we have observed the entire natural clinical course of one extremely indolent APL patient, who developed from pre‐APL stage (<20% promyelocytes in bone marrow) to overt‐APL stage (≥20% promyelocytes in bone marrow) with one nearly 2‐year latency. Strikingly, we identified one novel fusion RBCK1‐TRIB3 in the pre‐APL stage but not overt‐APL stage sample. It has been reported that TRIB3 stabilized PML‐RARA to driver APL progression, while RBCK1‐TRIB3 partially disrupted TRIB3WT expression, so it contributed to the deceleration of APL progression in this patient.