bluuebunny/crossref_metadata_2025_split huggingface dataset:
A unified computational view of DNA duplex, triplex, quadruplex and their donor–acceptor interactions
Authors: Park Gyuri , Kang Byunghwa , Park Soyeon V , Lee Donghwa , Oh Seung Soo 
Published: April 2021

AbstractDNA can assume various structures as a result of interactions at atomic and molecular levels (e.g., hydrogen bonds, π–π stacking interactions, and electrostatic potentials), so understanding of the consequences of these interactions could guide development of ways to produce elaborate programmable DNA for applications in bio- and nanotechnology. We conducted advanced ab initio calculations to investigate nucleobase model structures by componentizing their donor-acceptor interactions. By unifying computational conditions, we compared the independent interactions of DNA duplexes, triplexes, and quadruplexes, which led us to evaluate a stability trend among Watson–Crick and Hoogsteen base pairing, stacking, and even ion binding. For a realistic solution-like environment, the influence of water molecules was carefully considered, and the potassium-ion preference of G-quadruplex was first analyzed at an ab initio level by considering both base-base and ion-water interactions. We devised new structure factors including hydrogen bond length, glycosidic vector angle, and twist angle, which were highly effective for comparison between computationally-predicted and experimentally-determined structures; we clarified the function of phosphate backbone during nucleobase ordering. The simulated tendency of net interaction energies agreed well with that of real world, and this agreement validates the potential of ab initio study to guide programming of complicated DNA constructs.
Single-molecule imaging of epigenetic complexes in living cells: insights from studies on Polycomb group proteins
Authors: Brown Kyle , Andrianakos Haralambos , Ingersoll Steven , Ren Xiaojun 
Published: April 2021

Abstract
Chromatin-associated factors must locate, bind to, and assemble on specific chromatin regions to execute chromatin-templated functions. These dynamic processes are essential for understanding how chromatin achieves regulation, but direct quantification in living mammalian cells remains challenging. Over the last few years, live-cell single-molecule tracking (SMT) has emerged as a new way to observe trajectories of individual chromatin-associated factors in living mammalian cells, providing new perspectives on chromatin-templated activities. Here, we discuss the relative merits of live-cell SMT techniques currently in use. We provide new insights into how Polycomb group (PcG) proteins, master regulators of development and cell differentiation, decipher genetic and epigenetic information to achieve binding stability and highlight that Polycomb condensates facilitate target-search efficiency. We provide perspectives on liquid-liquid phase separation in organizing Polycomb targets. We suggest that epigenetic complexes integrate genetic and epigenetic information for target binding and localization and achieve target-search efficiency through nuclear organization.
Diagnostic accuracy and clinical impact of [18F]FET PET in childhood CNS tumors
Authors: Marner Lisbeth , Lundemann Michael , Sehested Astrid , Nysom Karsten , Borgwardt Lise , Mathiasen René , Wehner Peder S , Henriksen Otto M , Thomsen Carsten , Skjøth-Rasmussen Jane , Broholm Helle , Østrup Olga , Forman Julie L , Højgaard Liselotte , Law Ian 
Published: April 2021

Abstract

Background
Central nervous system (CNS) tumors cause the highest death rates among childhood cancers, and survivors frequently have severe late effects. Magnetic resonance imaging (MRI) is the imaging modality of choice, but its specificity can be challenged by treatment-induced signal changes. In adults, O-(2-[18F]fluoroethyl)-l-tyrosine ([18F]FET) PET can assist in interpreting MRI findings. We assessed the clinical impact and diagnostic accuracy of adding [18F]FET PET to MRI in children with CNS tumors.

Methods
A total of 169 [18F]FET PET scans were performed in 97 prospectively and consecutively included patients with known or suspected childhood CNS tumors. Scans were performed at primary diagnosis, before or after treatment, or at relapse.

Results
Adding [18F]FET PET to MRI impacted clinical management in 8% [95% confidence interval (CI): 4%-13%] of all scans (n = 151) and in 33% [CI: 17%-53%] of scans deemed clinically indicated due to difficult decision making on MRI alone (n = 30). Using pathology or follow-up as reference standard, the addition of [18F]FET PET increased specificity (1.00 [0.82-1.00] vs 0.48 [0.30-0.70], P = .0001) and accuracy (0.91 [CI: 0.87-0.96] vs 0.81 [CI: 0.75-0.89], P = .04) in 83 treated lesions and accuracy in 58 untreated lesions (0.96 [CI: 0.91-1.00] vs 0.90 [CI: 0.82-0.92], P &lt; .001). Further, in a subset of patients (n = 15) [18F]FET uptake correlated positively with genomic proliferation index.

Conclusions
The addition of [18F]FET PET to MRI helped discriminate tumor from non-tumor lesions in the largest consecutive cohort of pediatric CNS tumor patients presented to date.
Postmarket American Experience With Woven EndoBridge Device: Adjudicated Multicenter Case Series
Authors: Cherian Jacob , Chen Stephen R , Puri Ajit , Vakharia Kunal , Levy Elad , Eshraghi Sheila , Howard Brian M , Tong Frank C , Cawley C Michael , Gross Bradley , Alexander Matthew D , Grandhi Ramesh , Srinivasan Visish M , Burkhardt Jan-Karl , Johnson Jeremiah N , Kan Peter 
Published: April 2021

Abstract

BACKGROUND
The Woven EndoBridge (WEB) device was granted premarket approval in the United States following results of the Woven EndoBridge Intrasaccular Therapy (WEB-IT) study. WEB-IT reported excellent adequate angiographic occlusion of treated aneurysms with a high safety profile. These results were achieved, however, in the context of a prospective study with strict inclusion criteria and rigorous training support.

OBJECTIVE
To review early as-practiced clinical experience with the WEB device in the United States.

METHODS
Retrospective review across 6 institutions identified 91 patients undergoing 92 treatment sessions for WEB device placement in treatment of 91 intracranial aneurysms. Details regarding demographics, aneurysm characteristics, treatment considerations, clinical outcomes, and aneurysm occlusion were obtained and analyzed in a multicenter database. Angiograms from the index procedure and follow-up studies were reviewed by a blinded and independent adjudicator.

RESULTS
The middle cerebral, anterior communicating, and basilar artery complexes were the commonly treated locations. Eight patients presented with ruptured aneurysms. A mean of 1.2 devices were introduced per case. Technical failure without deployment of a WEB device occurred in 2% (2/92) of sessions. Complete aneurysm occlusion for patients with imaging follow-up was 49% (mean follow-up of 8 mo). Four aneurysms were retreated. 90% of patients had modified Rankin Scale ≤ 2 at last clinical follow-up with no mortalities.

CONCLUSION
Immediate postmarket experience with the WEB device, newly introduced at American centers, confirms safe procedural use, but long-term efficacy remains unclear. Early challenges include accurate sizing and device selection.
HSE Management Standards and burnout dimensions among rehabilitation professionals
Authors: Carpi M , Bruschini M , Burla F 
Published: April 2021

Abstract

Background
The Health & Safety Executive Indicator Tool (HSE-IT) is a standard-based questionnaire commonly used to assess work-related stress in organizations. Although the HSE-IT validity has been well documented and significant relationships have been observed between its scales and several work-related outcomes, to date there is no evidence concerning the relationships between the HSE-IT and burnout among healthcare workers.

Aims
To investigate the relationships between the HSE-IT subscales and burnout dimensions as measured by the Maslach Burnout Inventory (MBI) in a sample of Italian rehabilitation professionals employed in healthcare institutions.

Methods
An anonymous cross-sectional questionnaire was administered to a sample of Italian rehabilitation professionals including physical therapists, occupational therapists, psychiatric rehabilitation technicians and developmental psychomotor therapists. Associations between the HSE-IT and the MBI were analysed with multiple linear regression models.

Results
A total of 432 rehabilitation professionals completed the questionnaire and 14% of them showed high levels of burnout risk. Significant differences in the HSE-IT scores were found between workers at high risk of burnout and workers at low risk of burnout. Hierarchical regressions showed an association between the HSE-IT scales and the MBI factors: emotional exhaustion was associated with ‘demands’ and ‘role’, and both depersonalization and personal accomplishment were associated with ‘control’ and ‘role’.

Conclusions
This preliminary study showed the HSE-IT subscales are sensitive to burnout risk as measured by the MBI. The association found between the HSE-IT ‘demands’, ‘role’ and ‘control’ subscales and the MBI dimensions is significant but small. These findings might inform targeted burnout prevention.
Progressive Response to Repeat Application of Capsaicin 179 mg (8% w/w) Cutaneous Patch in Peripheral Neuropathic Pain: Comprehensive New Analysis and Clinical Implications
Authors: Freynhagen Rainer , Argoff Charles , Eerdekens Mariëlle , Engelen Sylvia , Perrot Serge 
Published: April 2021

AbstractObjectiveTo investigate the efficacy of repeated application of capsaicin 179 mg cutaneous patch in nonresponders to the first application.DesignPost hoc, as-treated analysis of two prospective trials (STRIDE and PACE) with 52-week follow-up.BlindingOpen-label.SettingMulticenter clinical trial.SubjectsSTRIDE: nondiabetic neuropathic pain; PACE: painful diabetic peripheral neuropathy.MethodsPatients were divided according to number of applications needed before attainment of a ≥30% reduction in average pain intensity (question 5 of the Brief Pain Inventory [BPI-Q5]). We assessed the change from baseline in average pain intensity (BPI-Q5), mean “interference with sleep” score, Patient Global Impression of Change, quality of life (QOL) via the EuroQol 5-dimension, and Self-Assessment of Treatment.ResultsIn STRIDE and PACE, respectively, n = 306 and n = 313 received the capsaicin patch; n = 60 and n = 96 had a response after the first application, n = 33 and n = 68 after the second, and n = 11 and n = 43 after the third. Among patients without a ≥30% reduction in pain intensity at 3 months, in STRIDE and PACE, respectively, 23.3% and 28.1% achieved a ≥30% reduction at 6 months, increasing to 33.9% and 45.7% at 12 months. Similar results were obtained when a decrease of ≥50% was used as the responder definition. Progressive improvements in pain intensity in slower responders reached levels similar to those in early responders at month 12 and were accompanied by improvements in sleep, QOL, and patient satisfaction.ConclusionsAlthough some patients with peripheral neuropathic pain experience rapid improvements with a single treatment of capsaicin 179 mg patch, some may require two or three treatments before an initial response is observed. Similar benefits for pain, sleep, and QOL can be achieved in early and late responders.
High-efficacy, high-manufacturability human VH domain antibody therapeutics from transgenic sources
Authors: Bélanger Kasandra , Tanha Jamshid 
Published: April 2021

Abstract
Interest in single-domain antibodies (sdAbs) stems from their unique structural/pronounced, hence therapeutically desirable, features. From the outset—as therapeutic modalities—human antibody heavy chain variable domains (VHs) attracted a particular attention compared with ‘naturally-occurring’ camelid and shark heavy-chain-only antibody variable domains (VHHs and VNARs, respectively) due to their perceived lack of immunogenicity. However, they have not quite lived up to their initial promise as the VH hits, primarily mined from synthetic VH phage display libraries, have too often been plagued with aggregation tendencies, low solubility and low affinity. Largely unexplored, synthetic camelized human VH display libraries appeared to have remediated the aggregation problem, but the low affinity of the VH hits still persisted, requiring undertaking additional, laborious affinity maturation steps to render VHs therapeutically feasible. A wholesome resolution has recently emerged with the development of non-canonical transgenic rodent antibody discovery platforms that appear to facilely and profusely generate high affinity, high solubility and aggregation-resistant human VHs.
Metonymies, metaphors and/or language reconsiderations for sustainability during COVID-19
Authors: Kahambing Jan Gresil S 
Published: April 2021

Abstract
Pandemic discussions employ language metaphors and metonymies to make sense of the coronavirus disease 2019 crisis. From commenting and proposing to revise terms such as social distancing, the war against the virus, to viewing mother nature as a killer, there are language reconsiderations to be made to avoid some disturbing mental imageries to picture a sustainable future. The Anthropocene geologic time and the improved environmental quality situate this backdrop. Language interventions make up as a vanishing mediation that will prompt a deeper understanding of the environment and nature as a whole.
The Politics of Alignment and the ‘Quiet Transgender Revolution’ in Fortune 500 Corporations, 2008 to 2017
Authors: Ghosh Apoorva 
Published: April 2021

AbstractThis study shows that when social movements achieve a general acceptance for the legitimacy of their cause in the institutional environment, they may start pursuing further demands by challenging their target entities through the ‘politics of alignment,’ meaning engaging these entities in professionally developed programs and demanding specific outcomes by introducing timed interventions in them. This study exemplifies this politics using the case of American LGBT workplace movement which used its Corporate Equality Index (CEI) program to extend reputational and economic benefits to its target entities—the Fortune 500 corporations—but also added an intervention to this program in 2011 to demand the adoption of gender transition-related health benefits by these corporations as a specific movement outcome which, if not met, would make these corporations lose the benefits they had been deriving through their performance in the CEI program. A longitudinal study of 456 Fortune 500 corporations from 2008 to 2017 conducted through hazard rate analysis indicates that corporations affected by this intervention, as well as by other movement factors, were the most likely to adopt these health benefits for their employees. Further quantitative analysis—using QCA—shows that early adoptions were explained largely by the LGBT workplace movement forces and the later adoptions by insider activism and isomorphic diffusion. These findings highlight that an incisive understanding of organizational change can be best gathered by examining social movements and institutional forces together.
Enhanced accumulation of pinosylvin stilbenes and related gene expression in Pinus strobus after infection of pine wood nematode
Authors: Hwang Hwan-Su , Han Jung Yeon , Choi Yong Eui 
Published: April 2021

Abstract
Pine wood nematodes (PWNs; Bursaphelenchus xylophilus) infect pine trees and cause serious pine wilt disease. Eastern white pine (Pinus strobus) has resistance to PWN. However, the detailed defense mechanisms of P. strobus against PWN are not well known. When P. strobus plants were infected with PWNs, the accumulation of stilbenoids, dihydropinosylvin monomethyl ether (DPME) and pinosylvin monomethyl ether (PME) was increased remarkably. Both DPME and PME had high nematicidal activity. The nematicidal activity of the two compounds was resulted in a developmental stage-dependent manner. Pinosylvin monomethyl ether was more toxic to adult PWNs than juveniles, whereas DPME was found more toxic to juvenile PWNs than the adults. The genes involved in PME and DPME biosynthesis such as phenylalanine ammonia-lyase (PAL), 4-coumarate-CoA ligase (4CL), pinosylvin synthase (STS) and pinosylvin O-methyltransferase (PMT) were isolated using de novo sequencing of the transcriptome in P. strobus. In addition, transcription factors (TFs; bHLH, MYB and WRKY) related to stilbene biosynthesis were isolated. qPCR analyses of the selected genes (PAL, 4CL, STS and PMT) including TFs (bHLH, MYB and WRKY) revealed that the expression level of the selected genes highly enhanced after PWN infection. Our results suggest that pinosylvin-type stilbenoid biosynthesis is highly responsive to PWN infection and plays an important role in PWN resistance of P. strobus trees.
Emergency Airway Management in Patients with COVID-19: A Prospective International Multicenter Cohort Study
Authors: Wong Danny J. N. , El-Boghdadly Kariem , Owen Ruth , Johnstone Craig , Neuman Mark D. , Andruszkiewicz Paweł , Baker Paul A. , Biccard Bruce M. , Bryson Gregory L. , Chan Matthew T. V. , Cheng Ming Hua , Chin Ki Jinn , Coburn Mark , Jonsson Fagerlund Malin , Lobo Clara A. , Martinez-Hurtado Eugenio , Myatra Sheila N. , Myles Paul S. , Navarro Guillermo , O’Sullivan Ellen , Pasin Laura , Quintero Kathleen , Shallik Nabil , Shamim Faisal , van Klei Wilton A. , Ahmad Imran 
Published: April 2021

Background
Tracheal intubation for patients with COVID-19 is required for invasive mechanical ventilation. The authors sought to describe practice for emergency intubation, estimate success rates and complications, and determine variation in practice and outcomes between high-income and low- and middle-income countries. The authors hypothesized that successful emergency airway management in patients with COVID-19 is associated with geographical and procedural factors.

Methods
The authors performed a prospective observational cohort study between March 23, 2020, and October 24, 2020, which included 4,476 episodes of emergency tracheal intubation performed by 1,722 clinicians from 607 institutions across 32 countries in patients with suspected or confirmed COVID-19 requiring mechanical ventilation. The authors investigated associations between intubation and operator characteristics, and the primary outcome of first-attempt success.

Results
Successful first-attempt tracheal intubation was achieved in 4,017/4,476 (89.7%) episodes, while 23 of 4,476 (0.5%) episodes required four or more attempts. Ten emergency surgical airways were reported—an approximate incidence of 1 in 450 (10 of 4,476). Failed intubation (defined as emergency surgical airway, four or more attempts, or a supraglottic airway as the final device) occurred in approximately 1 of 120 episodes (36 of 4,476). Successful first attempt was more likely during rapid sequence induction versus non–rapid sequence induction (adjusted odds ratio, 1.89 [95% CI, 1.49 to 2.39]; P < 0.001), when operators used powered air-purifying respirators versus nonpowered respirators (adjusted odds ratio, 1.60 [95% CI, 1.16 to 2.20]; P = 0.006), and when performed by operators with more COVID-19 intubations recorded (adjusted odds ratio, 1.03 for each additional previous intubation [95% CI, 1.01 to 1.06]; P = 0.015). Intubations performed in low- or middle-income countries were less likely to be successful at first attempt than in high-income countries (adjusted odds ratio, 0.57 [95% CI, 0.41 to 0.79]; P = 0.001).

Conclusions
The authors report rates of failed tracheal intubation and emergency surgical airway in patients with COVID-19 requiring emergency airway management, and identified factors associated with increased success. Risks of tracheal intubation failure and success should be considered when managing COVID-19.

Editor’s Perspective

What We Already Know about This Topic

What This Article Tells Us That Is New
The utility of prolactin serial sampling and the best prolactin cut-offs associated with persistent hyperprolactinemia
Authors: Cidade-Rodrigues Catarina , Cunha Filipe M. , Chaves Catarina , Silva-Vieira Margarida , Silva André , Garrido Susana , Martinho Mariana , Almeida Margarida 
Published: April 2021

Abstract

Background:
A single prolactin sampling is recommended for the diagnosis of hyperprolactinemia. We aimed to study the utility of the prolactin serial sampling and to determine the best cut-offs associated with persistent hyperprolactinemia.

Methods:
Retrospective study of hyperprolactinemic patients [referral prolactin (rPRL)] that underwent prolactin serial samplings. Prolactin at 0 minutes (PRL0′), 20 to 30, and 40 to 60 minutes. The lowest of these last 2 was defined as nadir prolactin (nPRL). Persistent hyperprolactinemia was defined as nPRL above normal. We excluded patients under dopamine receptor agonists. Receiver-operating characteristic (ROC) curves were used to determine the best rPRL and PRL0′ cut-offs predicting persistent hyperprolactinemia.

Results:
We studied 53 patients (3 males). Median rPRL 48.0 ng/mL (39.5–72.5), PRL0′ 34.3 ng/mL (18.0–50.8) and nPRL 29.5 ng/mL (11.4–44.4). PRL0′ was elevated in 35 (66.0%) patients and in 7 of them a normal nPRL was reached; therefore 28 (52.8%) had persistent hyperprolactinemia. The area under curve (AUC) for the association between rPRL and persistent hyperprolactinemia was 0.70 (95%CI: 0.56–0.84); best cut-off: 53.4 ng/mL [sensitivity 53.6%, specificity 80.0%, positive predictive value (PPV) 75.0%, and negative predictive value (NPV) 60.6%]. In the 35 patients with elevated PRL0′, the AUC was 0.92 (95%CI: 0.81–1.00); best cut-off: 35.2 ng/mL (sensitivity 85.7%, specificity 85.7%, PPV 60.0%, and NPV 96.0%).

Conclusions:
Approximately 1/3 of the patients reached a normal PRL0′. In an additional 20%, prolactin normalized after serial samplings. Patients with rPRL >53.4 ng/mL had 75% probability of having persistent hyperprolactinemia and those with PRL0′ <35.2 ng/mL had a 96% probability of not having persistent hyperprolactinemia.
Participating in vaccine research for COVID-19 in Senegal: trust and information
Authors: Ridde Valéry , Ba Mouhamadou Faly , Gaye Ibrahima , Diallo Amadou Ibra , Bonnet Emmanuel , Faye Adama 
Published: April 2021

AbstractThis research aims to understand the level and determinants of people’s willingness to participate in a vaccine trial for COVID-19 in Senegal. We conducted a telephone survey among a marginal quota sample of 607 people over 18 years of age. Only 44.3% of the participants wanted to participate in a vaccine trial for COVID-19, with females intending to participate more than males. Participants who intended to be vaccinated against COVID-19 (OR = 6.48, 95% CI [4.12-10.4]) and who thought that being infected with the coronavirus would have a significant impact on their health (OR = 2.34, 95% CI [1.57, 3.51]) were more likely to agree to take part in the COVID-19 vaccine research. Confidence in the vaccine, health personnel, and government in the fight against the pandemic are key factors in intending to participate in vaccine research in Senegal.