bluuebunny/crossref_metadata_2025_split huggingface dataset:
Fermentation Quality of Herbal Tea Residue and its Application in Fattening Cattle Under Heat Stress
Authors: Zhuang Xiaona , Chen Zujing , Sun Xiaohong , Li Fangjun , Luo Junyi , Chen Ting , Xi Qianyun , Zhang Yongliang , Sun Jiajie 
Published: September 2021

Abstract
Background: Herbal tea residue (HTR) is generally considered to be the waste of herbal tea beverage production while it still retains rich nutrients and active substances. The main aim of the present study was to investigate the effect of fermentation technology on improving the quality of HTRs, and focus on the fermented HTR-induced alleviation of summer heat stress in fattening cattle.Results: In this study, the waste HTR was fermented and then fed to a total of 45 fattening cattles that were divided into 3 groups (fermented HTR replaced 0%, 15%, 30% of Pennisetum purpureum respectively), and the feeding experiment was lasted for 40 days. The physiological indexes, growth performance and fecal microorganisms of fattening cattle were evaluated and results showed that fermented HTR could effectively reduce the respiratory rate and rectal temperature of fattening cattle under heat stress, increase the daily feed intake and daily gain, and improve the antioxidant content and blood immune index. In addition, we studied the fecal microorganisms composition of 6 fattening cattles in each group and found fermented HTR significantly changed the composition of fecal microorganisms and increased microbial diversity, and correlation analysis suggested that the bacteria were closely related to fecal SCFA levels of fattening cattle under heat stress. Conclusions: we suggested that 30% of fermented HTR can help maintain health of fattening cattle and alleviate the heat stress of fattening cattle by the change of intestine microorganisms.
Mendelian randomization informs shared genetic etiology underlying exposure and outcome by interrogating correlated horizontal pleiotropy
Authors: Cheng Qing , Chen Lin , Liu Jin 
Published: September 2021

Abstract
Mendelian randomization (MR) harnesses genetic variants as instrumental variables (IVs) to study the causal effect of exposure on trait/disease using summary statistics from genome-wide association studies. Classic MR assumptions are violated when IVs are associated with unmeasured confounders, i.e., when correlated horizontal pleiotropy (CHP) arises. Such confounders could be a shared gene or inter-connected pathways underlying exposure and outcome. We propose MR-CUE (MR with Correlated horizontal pleiotropy Unraveling shared Etiology and confounding), for estimating causal effect while identifying IVs with CHP and accounting for estimation uncertainty. For those IVs, we map their cis-associated genes and enriched pathways to inform shared genetic etiology underlying exposure and outcome. We apply MR-CUE to study the effects of interleukin 6 on multiple traits/diseases and identify several S100 genes involved in cross-traits etiology. We assess the effects of multiple exposures on type 2 diabetes across European and East Asian populations.
Spatial Heterogeneity and Socio-economic Correlates of Unmet Need for Spacing in India: Evidences From National Family Health Survey, 2015-16
Authors: Rahaman Margubur , Rana MD Juel , Roy Avijit , Chouhan Pradip 
Published: September 2021

Abstract
Background:The high unmet need for contraception among women who wants to delay their subsequent births for two or more years is a challenging issue in India. Therefore, the present study aims to display spatial heterogeneity and determine the associated factors underlying the unmet need for contraception among the currently married women aged 15-49 years with a demand for spacing births in India.Methods:The present study used data from the National Family and Health Survey, 2015-16. Unmet need for spacing (UNS) used as outcomes and socio-cultural and demographic factors as explanatory variables. Among the comprehensive family planning, 16.7% (56742 women) of currently married women had demand for spacing births were used for analysis. Univariate, bivariate with chi-square significance test, spatial natural break technique, and multivariate logistic regression were used to accomplish the study objectives. Results:Almost 17% of women had demand spacing out of total demand for family planning in India. High unmet need for contraception found among them except the women with higher-level education and wealth status. District level spatial heterogeneity of UNS shows 119 districts had very high unmet for spacing (69% and above). Results from multivariate logistic regression show that UNS's likelihood was noticeably high among the women with parity three or more (AOR: 1.42 [1.3-1.55]) compared to their counterparts. Similarly, 44% (AOR: 1.44[1.38-1.51]) more likely found among the OBC than General caste. Rural (AOR: 1.24[1.19-1.30]) women compared to urban, women living in South (AOR: 6.19 [5.76-6.65]), Central (AOR: 1.52 [1.43-1.61]), and West (AOR: 1.80 [1.68-1.92]) region compared to those from North had higher odds of UNS. Conclusion:Deal with the UNS in India requires scaling up women education, client choice-based services, campaign on benefits of spacing births and available contraception, and socio-economically backward group centric policies.
Screening in rheumatology: the impact on the everyday experience of patients in the Brazilian public health system
Authors: Serratto Varlei Antonoio , Kowacs Dora Pedroso , Kowalski Sérgio Candido 
Published: September 2021

Abstract
Background

In recent years, due to the increased incidence of rheumatologic diseases, the demand for consultations in rheumatology has risen. In the Brazilian public health system, patients with rheumatologic complaints are referred from primary care to specialized consultation, and must wait in a waiting list for the specialized care. In 2015, the waiting time for the first consultation in rheumatology, in Curitiba- Brazil, was 600 days. In attempt to reduce this time, in-person and remote screening was implemented that same year in a healthcare service in Curitiba. From 2015 to 2019 the variation in the number of patients in waiting lists for their first rheumatology consultation and the length of the waiting period for their first referral was evaluated.
Methods

Observational study in a time series. The patients in the study were referred to a primary healthcare center for an assessment of rheumatology in Curitiba, Brazil. The data was analyzed based on the first consultation with the rheumatologist. The variables were the number of patients in the waiting list and the waiting period for the appointment with the doctor. The research was carried out through a computerized system called “e-saude”[electronic health] runed by the Health Secretary of the Municipality of Curitiba. A comparison was made between the period of 2013–2015, in a model for treatment without screening, and 2015–2019, when the in-person and remote screening first began.
Results

Between 2015 and 2019 there was a reduction in the waiting list of 6,429 patients to 25 patients, while there was an average of 56.5 patients in the last two years;. With regard to the waiting period for the first appointment with the rheumatologist in 2019, there was a reduction of 600 days to a minimum of 4 days and a maximum of 52 days.
Conclusion

Both in-person and remote screening for asynchronous electronic consultations for rheumatologist in Curitiba led to a reduction in the waiting time and number of patients for the first appointment with the rheumatologist, proving to be a useful method for reducing the time and number of patients in the waiting list for a specialized consultation.
circPTP4A2-miR-330-5p-PDK2 Signaling Facilitates In Vivo Survival of HuMSCs on SF-SIS Scaffolds and Improves The Repair of Damaged Endometrium
Authors: Zheng Yuanyuan , Li Linhao , Bi Xuewei , Xue Ruyue 
Published: September 2021

Abstract
Background

Human umbilical cord MSCs (HuMSC)-based therapy has shown promising results in the treatment of intrauterine adhesions (lUA). In this study, our aim was to construct a HuMSC-seeded silk fibroin small-intestinal submucosa (SF-SIS) scaffold and evaluate the impact of repairing the damaged endometrium in an lUA mouse model.
Methods

To identify the functional effect of HuMSCs-silk cellulose (SF)- small-intestinal submucosa (SIS) scaffolds on the repair of damaged endometrium, a mouse lUA model was established in this study. The uterine morphology and fibrosis were evaluated by hematoxylin - eosin (H&E) staining and Masson staining. CircRNA sequencing, real-time PCR and RNA fluorescence in situ hybridization were used to screen and verify the potential circRNAs that involved in the repair of damaged endometrium by HuMSCs. Real time integrated cellular oxygen consumption rate (OCR) was measured using the Seahorse XF24 Extracellular Flux Analyser. The potential down-stream miRNAs and proteins of circRNAs were analyzed dual-luciferase report and Western Blot.
Results

We found that HuMSCs-SF-SIS not only increased the number of glands, but also reduced the ulcer area in the IUA model. Furthermore, we demonstrated that circPTP4A2 was elevated in the HuMSCs seeded on the SF-SIS scaffolds and stabilized the mitochondrial metabolism through miR-330-5p-PDK2 signaling, which contributes to endometrial repair progression.
Conclusion

In this study, we demonstrated that circPTP4A2 was elevated in the HuMSCs seeded on the SF-SIS scaffolds and stabilized the mitochondrial metabolism through miR-330-5p-PDK2 signaling, which contributes to endometrial repair progression. These findings demonstrate that HuMSC-seeded SF-SIS scaffolds are an encouraging method for the treatment of lUA.
Electrospun Composite Nanofibers Based on Poly (ε-caprolactone) and Styrax Liquidus (Liquidambar Orientalis Miller) as a Wound Dressing: Preparation, Characterization, Biological and Cytocompatibility Results
Authors: Demir Didem , Özdemir Sadin , Ceylan Seda , Yalcin M. Serkan , Sakım Burcu , Bölgen Nimet 
Published: September 2021

Abstract
In this study, styrax liquidus (sweet gum balsam) extracted from Liquidambar orientalis Mil. incorporated PCL fibrous scaffolds were prepared using the electrospinning method. The effects of the styrax liquidus content on the prepared scaffolds were investigated using different physico-chemical and morphological analyses. Then, the styrax-loaded nanofibers were examined for their antioxidant activity, anti-biofilm, metal chelating, antimicrobial and DNA cleavage properties. The results obtained from these studies showed that the nanofibers exhibited effective biological activity depending on the weight ratio of the styrax liquidus. In light of the data obtained from the characterization and biological studies, a sample with high ratio of balsam was built for determining the cytocompatibility analysis in vitro. The cytotoxicity studies of the selected membrane were conducted using mouse embryonic fibroblast cells. The fibrous scaffolds lead to increase the cell number as a result of high viability. According to the results, we propose a novel biocompatible electrospun hybrid scaffold with antioxidant and antimicrobial properties that can be used as wound healing material for potential tissue engineering applications.
Development of a Deep Learning System To Reduce The Time Needed for COVID-19 RT-PCR: Can We Use Deep Learning To Get RT-PCR Test Results of COVID-19 Faster?
Authors: LEE YOONJE , KIM Yu-Seop , Lee Da-in , Jeong Seri , Kang Gu-Hyun , Jang Yong Soo , Kim Wonhee , Choi Hyun Young , Kim Jae Guk , Choi Sang-hoon 
Published: September 2021

Abstract
Reducing the time to diagnose COVID-19 helps to manage insufficient isolation-bed resources and adequately accommodate critically ill patients in clinical fields. There is currently no alternative method to RT-PCR, which requires 40 cycles to diagnose COVID-19. We proposed a deep learning (DL) model to improve the speed of COVID-19 RT-PCR diagnosis. We developed and tested a DL model using the long-short term memory method with a dataset of fluorescence values measured in each cycle of 5,810 RT-PCR tests. Among the DL models developed here, the diagnostic performance of the 21st model showed an area under the receiver operating characteristic (AUROC), sensitivity, and specificity of 84.55%, 93.33%, and 75.72%, respectively. The diagnostic performance of the 24th model showed an AUROC sensitivity, and specificity of 91.27%, 90.00%, and 92.54%, respectively.
Effect of the New Zero-Tolerance Drinking and Driving Law on Hospital Admissions Due to Road Traffic Accidents in Brazil: an Interrupted Time Series Study From 2008 to 2019 Short Title New Zero-tolerance Drinking and Driving Law on Hospitalization in Brazil
Authors: Souza Cássia Rebeca Lima , Russo Letícia Xander , Silva Everton Nunes da 
Published: September 2021

Abstract
We investigated the effect of the new zero-tolerance drinking and driving law (Law 12760) on hospital admissions due to road traffic accidents in Brazil by using interrupted time series from 2008 to 2019. We used ICD-10 codes for land transport accidents (V00-V89). The hospitalization rate was calculated per 100,000 inhabitants. The sources were the Hospital Information System and the Brazilian Institute for Geography and Statistics. For Brazil, the hospitalization rate decreased by 0.400841 (p=0.052; 95% CI -0.8060128 to -0.0043307) in the first month of the intervention (December 2012), followed by a significant change in the hospitalization trend. Compared to the period prior to the intervention, the monthly trend was reduced by 0.048215 (p<0.01; 95% CI 0.0614524 to 0.0349775) in the post period. These results stand in agreement with subgroup analyses for the Brazilian regions, although North and Northeast regions did not immediately reduce hospitalization rates (level change). Our results suggested that 436,581 hospitalizations for land transport accidents were averted by the new zero-tolerance drinking and driving law from Dec. 2012 to Dec. 2019 in Brazil.
The Biological Investigations of a Benzoguanamine-based Nickel Complex and Its Application as a Capable Nano‐catalyst for the Synthesis of Benzimidazoles
Authors: Oliaee Sajjad , Habibi Davood , Heydari Somayyeh , Karamian Roya , Ranjbar Nika 
Published: September 2021

Abstract
The benzoguanamine-based nickel complex was prepared, characterized by several methods and its biological (anti-bacterial, anti-cancer and anti-oxidant) activities were investigated. Also, the benzoguanamine-based nickel complex was used as a capable heterogeneous nano-catalyst for the synthesis of diverse 2-phenyl-1H-benzo[d]imidazoles from the reaction of o-phenylenediamine (PhenDA) with various aldehydes in chloroform at reflux conditions.
Characteristics and Outcomes of Frail Patients with COVID-19 Admitted to ICU: An Individual Patient Data Meta-Analysis
Authors: Subramaniam Ashwin , Anstey Christopher , Curtis J Randall , Ashwin Sushma , REDDY Mallikarjuna PONNAPA , ALIBERTI Márlon Juliano Romero , AVELINO-SILVA Thiago Junqueira , Welch Carly , Koduri Gouri , Prowle John R , Wan Yize I , LAURENT Michaël R , Marengoni Alessandra , Lim Jun Pei , Pilcher David , Shekar Kiran 
Published: September 2021

Abstract
Purpose: Frailty is often used in clinical decision-making for patients with COVID-19, yet studies have found variable influence of frailty on outcomes in those admitted to the intensive care unit (ICU). In this individual patient data meta-analysis, we evaluated the characteristics, and outcomes of frail patients admitted to ICU with COVID-19.Methods: We contacted the corresponding authors of sixteen eligible studies published between December 1st 2019 and February 28th 2021 reporting the clinical frailty scale (CFS) in patients with confirmed COVID-19 admitted to ICU. Individual patient data was obtained from 7 studies. We classified patients as non-frail (CFS=1-4) or frail (CFS=5-8). The primary outcome was hospital mortality. We also compared the use of mechanical ventilation (MV) and the proportion of ICU bed-days between frailty categories. Results: Of the 2001 patients admitted to ICU, 388 (19.4%) were frail. Increasing age and sequential organ failure assessment (SOFA) score, CFS ≥4, use of MV, vasopressors, renal replacement therapy and hyperlactatemia were risk factors for death in a multivariable analysis. Hospital mortality was higher in frail patients (65.2% vs. 41.8%; p<0.001), with adjusted mortality increasing with a rising CFS score beyond 3. Younger and non-frail patients were more likely to receive MV. Frail patients spent less time on MV (median days [IQR] 9 [5-16] vs. 11 [6-18]; p=0.012) and accounted for only 12.3% of total ICU bed-days. Conclusion: Frail patients with COVID-19 were commonly admitted to ICU and had greater hospital mortality but spent relatively fewer days in ICU when compared with non-frail patients. Frail patients receiving MV were at greater risk of death than non-frail patients. Systematic review registration: Registration protocol in PROSPERO (CRD42020224255).
Wearable Multimodal - Serious Game System For Hand and Cognitive Rehabilitation After Stroke
Authors: Song Xinyu , de Ven Shirdi Shankara van , Kang Peiqi , Gao Qinghua , Chen Shugeng , Jia Jie , Shull Peter Bradley 
Published: September 2021

Abstract
Objective: Stroke often leads to both motor control and cognitive dysfunction, and effective rehabilitation requires keeping patients engaged and motivated. We introduce a wearable multimodal system based on force myography, electromyography, and inertial sensing with two associated serious games for stroke rehabilitation of twelve hand movements related to activities of daily living and the Fugl Meyer Assessment.Methods: In the ‘Find the Sheep’ serious game, patients performed corresponding hand movements to select the correct sheep card, and in the ‘Best Salesman’ serious game, patients performed corresponding hand movements to grab specific food and drink items in a store. A multi-sensor fusion model was developed for movement classification via linear discriminant analysis. Ten stroke patients with mild to moderate motor impairments (Brunnstrom Stage for Hand II-VI) performed validation testing, and effectiveness was evaluated by movement classification accuracy and qualitative patient questionnaires.Results: Classification accuracy for twelve movements using combined force myography, electromyography, and inertial sensing was 81.0%, and accuracies for using electromyography, force myography, or inertial sensing alone were 69.6%, 63.2%, and 47.8%, respectively. All patients reported that they were more enthusiastic about rehabilitation while playing serious games than conventional rehabilitation, and a majority reported the wearable multimodal-based system was easier to wear than a sensorized data glove. Significance: Results showed that multi-sensor fusion could improve hand gesture classification accuracy for stroke patients and demonstrated that the proposed wearable multimodal-serious game system could potentially facilitate upper extremity rehabilitation and cognitive training after stroke.
Non-invasive Monitoring of Core Body Temperature for Targeted Temperature Management in Post-Cardiac Arrest Care
Authors: Fiorini Kyle , Tamasi Tanya , Dorie Justin , Hegazy Ahmed F. , Lee Ting-Yim , Slessarev Marat 
Published: September 2021

Abstract
BackgroundAccurate monitoring of core body temperature is integral to targeted temperature management (TTM) following cardiac arrest. However, there are no reliable non-invasive methods for monitoring temperature during TTM. We compared the accuracy and precision of a novel non-invasive Zero-Heat Flux Thermometer (SpotOnä) to a standard invasive esophageal probe in a cohort of patients undergoing TTM post cardiac arrest. MethodsWe prospectively enrolled 20 patients undergoing post-cardiac arrest care in the intensive care units at the London Health Sciences Centre in London, Canada. A SpotOnä probe was applied on each patient’s forehead, while an esophageal temperature probe was inserted, and both temperature readings were recorded at 1-minute intervals for the duration of TTM. We compared the SpotOnä and esophageal monitors using Bland-Altman analysis and Pearson correlation, with accuracy set as a primary outcome. Secondary outcomes included precision and correlation. Bias exceeding 0.1°C and limits of agreement exceeding 0.5°C were considered clinically important.ResultsSixteen (80%) of patients had complete data used in the final analysis. The median (interquartile range) duration of recording was 38 (12-56) hours. Compared to the esophageal probe, SpotOnä had a bias of 0.05 ± 0.35ºC and 95% limits of agreement of -0.64 to 0.74 ºC. Pearson correlation coefficient was 0.98 (95% confidence interval 0.9796-0.9805), with a two-tailed p-value of <0.0001. ConclusionThe SpotOnä is an accurate method that may enable non-invasive monitoring of core body temperature during TTM, although its precision is slightly worse than the pre-defined 0.5°C when compared to invasive esophageal probe.
Characterization of Methyltransferase from Apramycin Biosynthetic Gene Cluster and Improvement Production of Apramycin in Engineered Streptoalloteichus Tenebrarius
Authors: Sun Junyang , Gao Hongjing , Yan Danyang , Liu Yu , Ni Xianpu , Xia Huanzhang 
Published: September 2021

Abstract
BackgroundApramycin is a structurally unique aminoglycoside, used in veterinary medicine or the treatment of Salmonella, Escherichia coli and Pasteurella multocida infections in farm. Although discovered and used many years ago, many biosynthetic steps of apramycin are still obscure. ResultsIn this study, we identified a HemK family methyltransferase, aprI, involved in apramycin biosynthesis. The function of aprI was studied by using gene disruption and biochemical experiments, and a new aminoglycoside antibiotic demethyl-apramycin was purified from aprI disruption strain. Experiments proved that AprI converted demethyl-aprosamine to aprosamine in vitro. Based on this, the apramycin production strain was improved by overexpression the AprI to decrease the impurity production. ConclusionsWe have identified aprI is a 7’-N-methyltransferase gene in apramycin biosynthesis and confirmed the substrate of methyltransferase. Engineering of aprI resulted in a strain producing a new aminoglycoside demethyl-apramycin and apramycin mono-producing strain with less impurity production. Finally, the yield of demethyl-apramycin in apramycin mono-producing strain decreased from 196±36 mg/L to 51±9 mg/L, and the yield of apramycin increased from 2227±320 mg/L to 2331±210 mg/L.