bluuebunny/crossref_metadata_2025_split huggingface dataset:
Caveolin-Mediated Endocytosis: Bacterial Pathogen Exploitation and Host–Pathogen Interaction
Authors: Barman Dibyasri , Drolia Rishi 
Published: December 2024

Within mammalian cells, diverse endocytic mechanisms, including phagocytosis, pinocytosis, and receptor-mediated endocytosis, serve as gateways exploited by many bacterial pathogens and toxins. Among these, caveolae-mediated endocytosis is characterized by lipid-rich caveolae and dimeric caveolin proteins. Caveolae are specialized microdomains on cell surfaces that impact cell signaling. Caveolin proteins facilitate the creation of caveolae and have three members in vertebrates: caveolin-1, caveolin-2, and caveolin-3. Many bacterial pathogens hijack caveolin machinery to invade host cells. For example, the Gram-positive facultative model intracellular bacterial pathogen Listeria monocytogenes exploits caveolin-mediated endocytosis for efficient cellular entry, translocation across the intestinal barrier, and cell–cell spread. Caveolin facilitates the internalization of group A streptococci by promoting the formation of invaginations in the plasma membrane and avoiding fusion with lysosomes, thereby aiding intracellular survival. Caveolin plays a crucial role in internalizing and modulation of host immune responses by Gram-negative bacterial pathogens, such as Escherichia coli K1, Klebsiella pneumoniae, Pseudomonas aeruginosa, and Salmonella enterica serovar Typhimurium. Here, we summarize how bacterial pathogens manipulate the host’s caveolin system to facilitate bacterial entry and movement within and between host cells, to support intracellular survival, to evade immune responses, and to trigger inflammation. This knowledge enhances the intervention of new therapeutic targets against caveolin in microbial invasion and immune evasion processes.
Existence of Bounded Solutions for a Class of Degenerate Fourth-Order Elliptic Equations with Convection Terms
Authors: D’Asero Salvatore 
Published: December 2024

This paper deals with the existence of bounded and locally Hölder continuous weak solutions of the following nonlinear fourth-order Dirichlet problem: ∑|α|=1,2(−1)|α|DαAα(x,u,D1u,D2u)−Eα(x) |u|λ(pα−1) signu=f in Ω, where the coefficients Aα satisfy a strengthened degenerate coercivity condition.
Indomethacin-Induced Gastric Ulcer in Rats: Gastroprotectivity of Muscari neglectum in Water
Authors: Soydan Menekse , Arabaci Gulnur , Utlu Necati , Halici Mesut Bünyami , Aktas Senocak Esra , Kiliçlioglu Metin 
Published: December 2024

Background and Objectives: The plant Muscari Mill. is employed in both raw and cooked forms for the treatment of gastric diseases, as an expectorant, and for the treatment of warts and the enhancement of urine. A review of the scientific literature revealed no studies investigating the effect of Muscari neglectum (MN) water extract on gastric diseases. The objective of this study was to determine the effect of a water extract of the MN plant on indomethacin-induced gastric ulcer in rats, using a series of biochemical (SOD, CAT, GSH and MDA levels) and histopathological parameters. Methods: 60 male Sprague Dawley rats were utilized for the purposes of evaluating the acute toxicity and gastric ulcer models, with a total of 36 rats employed for these experiments (n = 6). The rats were divided into six groups: intact; indomethacin; famotidine; indomethacin and MN (100, 200, 400 mg/kg). Results: The Gastric tissue examinations at biochemical, macroscopic and pathological levels showed that MN extracts effectively prevented indo-methacin-induced gastric mucosal damage. The 400 mg/kg dose exhibited the most effective antiulcer effect, with a 69% protective efficacy. This dose caused an increase in the SOD, CAT and GSH levels and a decrease in the MDA levels compared to the IND group. Furthermore, an LC-MS/MS analysis was conducted on the water extract of MN, resulting in the identification of 14 phenolic compounds. Conclusions: Biochemical analyses and histopathological examinations demonstrated that the water extract of MN exhibited a beneficial protective effect against gastric ulceration due to its high antioxidant content.
Studi In Silico : Aktivitas Antibakteri Senyawa Resveratrol Terhadap Bakteri Salmonella typhi Penyebab Demam Tifoid
Authors: Sinaga Jonathan Cavin Ezra , Kumaunang Maureen , Tuther Paulix 
Published: December 2024

ABSTRAK
Demam tifoid merupakan salah satu penyakit infeksi yang menyerang sistem pencernaan oleh adanya aktivitas bakteri Salmonella typhi. Pengobatan demam tifoid menggunakan antibiotik yang dapat menimbulkan resistensi bakteri, sehingga penemuan senyawa sebagai alternatif obat demam tifoid masih terus dikembangkan hingga saat ini. Penelitian ini bertujuan untuk mengetahui pengaruh penambatan senyawa resveratrol terhadap pertumbuhan dari bakteri Salmonella typhi. Jenis penelitian ini menggunakan metode penelitian penambatan molekul (molecule docking). Hasil dari molecular docking didapatkan binding afiinity interaksi ligan dan reseptor yaitu -7.8 kkal/mol, maka senyawa resveratrol dapat sebagai penghambat bakteri Salmonella typhi.

ABSTRACT
Typhoid fever is an infectious disease that attacks the digestive system due to the activity of Salmonella typhi bacteria. Treatment of typhoid fever uses antibiotics which can cause bacterial resistance, so the discovery of compounds as alternative drugs for typhoid fever is still being developed today. This research aims to determine the effect of anchoring the resveratrol compound on the growth of Salmonella typhi bacteria. This type of research uses the molecular docking research method. The results of molecular docking showed that the binding affinity of the ligand and receptor interaction was -7.8 kcal/mol, so the resveratrol compound could act as an inhibitor for Salmonella typhi bacteria.
Analyzing Cost Efficiency Elements in Virtual Teams Application : A Systematic Review
Authors: Merrylien Rayo Natashia , Fabiola Rayo Edwina , Ronald Sumanti Elvis 
Published: December 2024

Purpose: This study aims to understand the critical elements influencing the cost-effectiveness of virtual teams and their strategic application in enterprises.
Research methodology: The research uses a literature review to assess the organizational dynamics, money allocation, and efficiency of virtual teams.
Results: The study finds that investing in technology, especially communication platforms, is crucial. Savings are identified in team development, office space, travel costs, and communication expenses.
Limitations: The research relies heavily on prior studies, limiting its generalizability and requiring adaptation to rapid technological changes.
Influence of Brand Recognition on Market Leadership in The Mobile Money Industry in Kenya
Authors: Kariuki Joan , Wanjira Jane , Njuguna Reuben 
Published: December 2024

This study sought to establish the influence of brand recognition on market leadership in the mobile money industry. The study was guided by three theoretical foundations including Patterson’s Theory of First movers, Resource-Based Theory, and Theory of Innovation Diffusion. The study was guided by the positivism philosophy. The study adopted both descriptive and explanatory survey designs. Descriptive research design enables one to gather quantifiable information that is applicable for statistical inference through data analysis. The target population for this study comprised of 96 managers in the following departments: human resource, operations, finance and technology in the three mobile money providers. The study employed a census sampling technique because the target population was small. Data was collected using structured questionnaires and key informant interviews. The data collection instruments were pretested for validity and reliability. Content validity and construct validity were used in testing for validity of the data collection instruments whereas Cronbach's alpha was used in testing for reliability. Data from key informants was analyzed using content analysis. Descriptive statistics (frequencies, percentages and means) were used to summarize data while inferential statistics were used to test the relationships and evaluate effect of independent variables on the dependent variable. The findings are presented in Tables and Figures. The study found out that brand recognition significantly contributes to market leadership (p<0.05). The regression analysis further showed that brand recognition explain 42.1% of the variations in market leadership in the mobile money industry in Kenya (R2=0.421). The study recommends that companies in Kenya's mobile money industry prioritize building brand recognition to strengthen their market leadership. Additionally, companies should focus on cultivating strong customer relationships, leveraging first-mover advantages, and adhering to regulatory compliance and industry standards. Finally, fostering a culture of innovation, monitoring market trends, and adopting sustainable growth strategies are crucial for maintaining long-term competitiveness and social impact within the dynamic mobile money landscape of Kenya.
Dekonstruksi Kolak Pisang Menjadi Milkshake
Authors: Aulia Nada Cinta , Zakiah Siti 
Published: December 2024

Tujuan dari penelitian ini adalah untuk menentukan tingkat kesukaan rasa, aroma, warna, dan tekstur dari produk Dekontruksi Kolak Pisang menjadi Milkshake serta seberapa baik hasilnya diterima masyarakat. Di jaman sekarang , makanan sudah lain menjadi kebutuhan pokok saja tetapi sudah menjadi gaya hidup yang modern bagi pecinta kuliner. Karena tren budaya baru yang masuk ke Indonesia, kuliner khas Indonesia mulai kehilangan popularitasnya. Akibatnya, peneliti tertarik untuk mendekonstruksi masakan tradisional Indonesia. Metode yang digunakan adalah metode Eksperimental dengan tahap dekonstruksi. Peneliti akan menyebar kuesioner untuk mengetahui daya terima konsumen untuk mengumpulkan pendapat tentang dekonstruksi masakan khas indonesia menjadi minuman dingin yaitu Milkshake. Digunakan panelis sebanyak 30 orang.Hasil penelitian menunjukkan bahwa Milkshake Kolak Pisang menggabungkan rasa tradisional dengan rasa krim dan susu. Pisang yang lembut, rasa gula merah yang manis, dan bau santan membuat pelanggan memiliki pengalaman yang unik dan memuaskan. Studi ini membantu perkembangan kuliner dengan menggabungkan inovasi dan tradisi. Milkshake Kolak Pisang dapat menjadi alternatif yang menarik bagi Pecinta Kolak Pisang yang ingin mencoba sesuatu yang baru. Selain itu, penelitian ini menawarkan kesempatan untuk mempelajari lebih lanjut tentang cara yang inovatif untuk mengembangkan produk makanan tradisional.Produk dapat diterima dan disukai oleh konsumen berdasarkan hasil uji organoleptik untuk mengetahui daya terima konsumen.Diharapkan penelitian ini akan bermanfaat bagi konsumen yang tertarik dengan milkshake kolak pisang.
Manifestation of a complex of Fusarium solani (Mart.) Sacc. species on seeds and seedlings of cereals, legumes, oilseeds and cereals
Authors: Rozhkova T. , Biliavska L. , Iutynska H. , Tytova L. , Leonova N. , Loboda M. , Sylchuk A. , Rozhkova Ye. 
Published: December 2024

Goal. To establish the peculiarities of infection of seeds and seedlings of cereals, legumes, oilseeds and cereals by a complex of FSSC species, to determine the factors influencing the spread of the pathogen.
Methods. Laboratory methods: germination of crop seeds in different soil samples and study of seedling development; identification of fungal species that formed mycelium layers during seed germination; analysis of soil mycobiota; determination of drug efficacy.
Results. In 2023—2024, the seeds of winter wheat, spring barley, winter rye, corn, sunflower, soybeans, beans, peas, millet, and buckwheat were studied for FSSC infection during germination in soil. Seeds were germinated in soils from different regions of Ukraine. The main symptoms of infection with the fungi of the F. solani species complex were studied. FSSC were isolated into pure culture to study their cultural and morphological characteristics. In order to find control of these phytopathogens, the factors influencing their spread were investigated: soil samples, precursor, crop genotype, seed treatment, and the use of various types of explosives.
Conclusions. FSSCs cause the formation of fluffy white-pink mycelium layers and are often the reason why seeds do not germinate in the soil and necrosis forms on the seedlings. Differences in the color of colonies and sporulation structure were found when fungi were isolated from ungerminated seeds of different crops. The number of FSSC species was significantly influenced by the soil sample, precursor, crop genotype, seed treatment, and the effects of various weapons. The largest number of fungi was found during germination of rye seeds in sandy loam soil of Kyiv region, which explained the lowest germination rate of seeds in the compared soil samples. The study of soybean seed germination in soils from Poltava region revealed the best predecessor for this crop — corn, which proved to be the most resistant to the development of the F. solani species complex. It was shown that the bean variety Nautica was almost five times more resistant to FSSC than T9905. The efficiency of using a mixture of preparations (Merivon® Pro, KS; 0.3—0.4 l/t; Ekovital and Vitazim; 1 l/t) against Fusarium bacterial infection of soybean seeds was 46.3%. The consequences of the use of explosive weapons led to quantitative and qualitative changes in the mycobiota of the studied samples of chernozems from Sumy and Kharkiv regions.
The influence of sowing rates on winter barley infection by Pyrenophora teres Drechsler under conditions of Steppe of Ukraine
Authors: Tіtov I. 
Published: December 2024

Goal. To determine the optimal seeding rates of winter barley to reduce the development of reticular helminthosporiosis (Pyrenophora teres Drechsler).
Methods. Observations of crops of zoned winter barley varieties were carried out during the period from germination to milky-wax ripeness. The development of plant damage by the pathogen was determined on a six-point scale, taking into account the percentage of the affected leaf area. The basis of the methodological research is: empirical (field sampling, measurement of the object of study), theoretical (hypothesis formulation and conclusions based on the results of research), mathematical.
Results. The damage to plants by the pathogen Pyrenophora teres Drechsler depended on the seeding rate. It was found that with an increase in sowing density, the degree of disease development increases. During the years of research, the development of the disease when sowing winter barley with 5.5 million seeds/ha was 3.0% higher compared to the norm of 4.5 million seeds/ ha and 1.2% higher than at the norm of 3.5 million. The grain yield was 0.18 t/ha less than the norm of 4.5 million seeds/ha and 0.14 t/ha less than the norm of 3.5 million seeds/ha. It was proved that a decrease in the seeding rate led to a decrease in the weight of 1000 grains by an average of 0.6 g. At the same time, there was a decrease in yield by 0.22 t/ha compared to sowing 4.5 million seeds/ ha.
Conclusions. It was found that the optimal seeding rate for winter barley in Odesa region is 4.5 million seeds per 1 ha. At reduced and increased seeding rates, the damage to plants by the pathogen Pyrenophora teres Drechsler increases by 1.9—3.1%, respectively. Such sowings lead to a decrease in grain yield by 0.22—0.30 t/ ha compared to the sown areas where 4.5 million/ha of grains were sown.
Determination of atrazine residues in alfalfa plant matrix by ultra-high-performance liquid chromatography and tandem mass spectrometry
Authors: Zhu Yinghui , Rozhkova T. 
Published: December 2024

Goal. The purpose of this study was to verify the effectiveness of determination of atrazine residues in alfalfa using ultra-high-performance liquid chromatography and tandem mass spectrometry.
Methods. To obtain samples, alfalfa was sprayed once in the field. Plant material was prepared before determination of atrazine residues. For this, the samples (0.2 g) were crushed in liquid nitrogen, 5 ml of water, acetonitrile, and 1% acetic acid were added for extraction. For purification and concentration, 1 mL of supernatant was added to 50 mg of C18, 50 mg of PSA, and 100 mg of anhydrous magnesium sulfate. Chromatography and mass spectrometry conditions: flow rate of 0.4 mL/ min at 40°C was maintained for separation using Agilent, C18 chromatographic columns; detection conditions — desolvation gas temperature and flow 350°C, respectively; nebulizer gas pressure 40.0 psi; ion spray voltage 4500 V; the temperature of the heater MS 1 and MS 2 is 300°C; target compound was quantified using MS/MS acquisition parameters (MRM mode).
Results. In the range of 0.01—1.0 mg/L, the mass concentration of atrazine herbicide and the corresponding peak area showed a good linear relationship, with a coefficient of determination of 0.9999. When adding atrazine at 0.1—5.0 mg/ kg, the average value of its content in alfalfa plants was 102.6%—107.5%, and the relative standard deviation was 0.28%—6.85%. The final residue detection result in alfalfa by ultra-high performance liquid chromatography-tandem mass spectrometry was 0.011 mg/kg (the maximum permissible level in China for cereals is 0.05 mg/kg).
Conclusions. The method has simple pretreatment operation, high average detection value and precision, good separation and purification effect, short analysis time, and is suitable for the detection of atrazine. It combines two traditional methods into one that is simpler and faster. The sensitivity and reproducibility meet the requirements of general toxicological tests, which have been confirmed by real tests. The method is suitable for the actual needs of detecting herbicide residues in alfalfa samples.
Pelatihan Pembuatan Produk Pempek Frozen dan Digital Marketing pada Warga Binaan Lapas Kelas IIA Bekasi
Authors: Hamidatun Hamidatun , Azis Lukman , Utami Annisa Retno 
Published: December 2024

<p><em>Pempek merupakan makanan tradisional Palembang yang sudah dikenal oleh masyarakat luas dan dapat dikembangkan menjadi peluang usaha. Warga binaan Lembaga Pemasyarakatan (Lapas) Kelas IIA Bekasi memiliki potensi dalam pengembangan ini karena terdapat kelompok keterampilan tata boga yang menghasilkan berbagai produk pangan salah satunya pempek. Namun, pempek yang dihasilkan termasuk pempek pangan siap saji dengan umur simpan pendek dan pemasarannya masih terbatas di dalam lapas. Warga binaan dan petugas lapas belum memiliki keterampilan pembuatan pempek frozen dan digital marketing. Tujuan kegiatan ini adalah untuk meningkatkan pengetahuan dan keterampilan warga binaan dan petugas lapas tentang pembuatan produk pempek frozen dan pemasaran secara digital. Kegiatan ini dilaksanakan selama 5 minggu dengan jumlah peserta 20 orang yang terdiri dari 15 warga binaan dan 5 petugas lapas. Metode pelaksanaan terdiri dari (1) pelatihan dan praktik pembuatan pempek frozen meliputi pembuatan produk, pengemasan vakum dan pembuatan label kemasan, (2) pelatihan digital marketing, dan (3) evaluasi dengan pre-test dan post-test dengan rerata peningkatan sebesar 24,92%. Warga binaan mampu membuat pempek frozen yang dikemas vakum, label kemasan dengan desain yang menarik dan sesuai aturan serta mampu membuat foto produk yang menarik dan katalog produk sebagai media promosi digital.</em></p>
Rekayasa Ulang Proses Bisnis Kelompok Tani Parijotho Muria Kudus Melalui Penerapan Mesin Pengering Cerdas
Authors: Riza Radyanto Mohammad , Ma’sum Muhammad Ali , Lusiana Veronica 
Published: December 2024

Desa Colo Kecamatan Dawe Kabupaten Kudus merupakan sentra budidaya tanaman Parijoto (Medinella Speciosa Blume) buah endemik yang banyak tumbuh di lereng Gunung Muria pada ketingggian 900-1200 mdpl.Mitra pengabdian adalah Kelompok Tani Parijotho Muria Kudus yang membudidayakan buah parijoto hingga pengolahan hasil panen.Dalam proses produksi dan budi dayanya kelompok ini dihadapkan pada permasalahan rendahnya produktivitas pengolahan hasil panen dan keseragaman mutu produk yang tidak sama karena belum memiliki standar mutu.Hal tersebut disebabkan karena alat produksi dan pemrosesannya masih menggunakan cara yang sederhana dan manual karena keterbatasan sumber daya.Metode kegiatan pengabdian yang dilakukan adalah rekayasa ulang proses bisnis dalam bentuk diseminasi hasil penelitian dosen berupa pemanfaatan alat produksi cerdas berbasis Internet Of Things (IoT) salah satunya adalah mesin pengering buah parijoto yang menggunakan sistem arduino dengan tujuan agar petani meningkatkan produktivitasnya dalam mengolah hasil panen.Selain itu, melalui kegiatan pelatihan dan sosialisasi pentingnya pemanfaatan teknologi cerdas dalam proses pengolahan hasil panen, paska pengabdian masyarakat petani memiliki peningkatan level keberdayaan mitra dari sisi aspek produksi, manajemen dan sosial kemasyarakatan berupa meningkatnya pengetahuan dan ketrampilan.Penerapan teknologi cerdas pada alat produksi memberikan dampak signifikan berupa: pemanfaatan alat pengering berteknologi IoT, terjadi kenaikan pasokan bahan baku dari semula 50% menjadi 70 % , produktivitas meningkat dari 10 liter per hari menjadi 15 liter per hari. Hasil dari kegiatan pengabdian ini berimplikasi pada peningkatan produktivitas dan mutu produk hasil panen melalui standarisasi mutu dan proses produksi
Penyusunan Profil Desa dalam Mendukung Kemandirian Desa Lubuk Enau Kecamatan Lembak Kabupaten Muara Enim
Authors: Dara Kospa Herda Sabriyah , Haidir Hala 
Published: December 2024

Berdasarkan hasil analisis permasalahan mitra Pemerintah Desa Lubuk Enau belum memiliki profil desa yang terperinci dan akuntabel, sedangkan pembangunan desa yang efektif dan tepat sasaran membutuhkan data desa yang terinventarisasi dengan baik dan mutakhir. Profil desa terdiri dari data dasar keluarga, data potensi desa, perkembangan dan permasalahan desa, penguasaan aset ekonomi dan sosial keluarga, serta partisipasi masyarakat desa. Profil desa berisi data dasar yang penting dalam proses penyusunan rencana pembangunan desa, sehingga dapat digunakan sebagai pedoman dalam program peningkatan kapasitas penduduk desa dan pembangunan fisik desa. Oleh sebab itu, PkM penyusunan Profil Desa Lubuk Enau dilakukan guna membantu dan memfasilitasi pemerintah Desa Lubuk Enau dalam melengkapi dan memutakhirkan data profil desanya. Metode yang digunakan dalam penyusunan profil desa ini dimulai dari penyiapan instrumen, pelaksanaan pengumpulan data, pengolahan dan interpretasi data, serta publikasi data. Luaran dari kegiatan PkM ini berupa dokumen Profil Desa Kabupaten Lubuk Enau yang melengkapi dan menyempurnakan data desa sebelumnya. Disertai hasil observasi lapangan, survei dan wawancara dengan masyarakat, dokumen ini dilengkapi dengan foto deskripsi dan hasil pemetaan menggunakan ArgGIS. Melalui progam PkM penyusunan profil desa ini diharapkan dapat membantu perangkat Desa Lubuk Enau dalam menyediakan basis data yang mutakhir dan akuntabel untuk perencanaan pembangunan desa, dan dapat menggali potensi desa.