bluuebunny/crossref_metadata_2025_split huggingface dataset:
Oral Treatment with Angiotensin-(1-7) Attenuates the Kidney Injury
Induced by Gentamicin in Wistar Rats
Authors: Pacheco Lílian Fernanda , de Castro Carlos Henrique , Dutra João Batista Rodrigues , de Souza Lino Ruy , Ferreira Patrícia Maria , dos Santos Robson Augusto Souza , Ulhoa Cirano José 
Published: November 2021

Background:
Acute Kidney Injury (AKI), a common disease of the urinary system, can
be induced by high doses of gentamicin (GM). The renin-angiotensin system exerts a key role in
the progression of the AKI since elevated intrarenal levels of Ang II, and ACE activity is found in
this condition. However, it is unknown whether oral administration of angiotensin (Ang)-(1-7), a
heptapeptide that evokes opposite effects of Ang II, may attenuate the renal injuries induced by gentamicin.

Objectives:
To evaluate the effects of Ang-(1-7) on GM-induced renal dysfunction in rats.

Methods:
AKI was induced by subcutaneous administration of GM (80 mg/Kg) for 5 days. Simultaneously,
Ang-(1-7) included in hydroxypropyl β-cyclodextrin (HPβCD) was administered by gavage
[46 μg/kg HPβCD + 30 μg/kg Ang-(1-7)]. At the end of the treatment period (sixth day), the
rats were housed in metabolic cages for renal function evaluation. Thereafter, blood and kidney
samples were collected.

Results:
Ang-(1-7) attenuated the increase of the plasmatic creatinine and proteinuria caused by
GM but did not change the glomerular filtration rate nor tubular necrosis. Ang-(1-7) attenuated the
increased urinary flow and the fractional excretion of H2O and potassium observed in GM rats but
intensified the elevated excretion of sodium in these animals. Morphological analysis showed that
Ang-(1-7) also reduced the tubular vacuolization in kidneys from GM rats.

Conclusion:
Ang-(1-7) promotes selective beneficial effects in renal injuries induced by GM.
Optimized Expression of Recombinant Human NIMA-Related Kinase 7
(NEK7) with A Higher Purity in Escherichia coli
Authors: Zhang Xing-Jie , Wang Ting-Ting , Pu Yu-Kun , Zeng Lin , Zhang Rui-Han , Li Xiao-Li , Ji Xu , Xiao Wei-Lie 
Published: November 2021

Background:
NIMA (never in mitosis, gene A) serine/threonine kinase 7 (NEK7) is a
regulator of mitosis spindle in mammals and is considered as a drug target of inflammasome related
inflammatory diseases. However, most commercially available or reported recombinant NEK7
proteins are either inactive or have low purity. These shortcomings limit the pharmacological
studies and development of NEK7 inhibitors.

Objective:
To elucidate what causes the NEK7 low purity in E. coli, and optimize a protocol to improve
the protein purity.

Methods:
A comparative study of expression full length NEK7 with an N-terminal His-tag or a Cterminal
His-tag was performed. His-affinity resin, ion exchange and gel filtration chromatography
were used to purify NEK7. The protein was identified by mass spectrometry. The activity and folding
of NEK7 were evaluated by chemiluminescent assay and thermal shift assay.

Results:
Our results demonstrated that N-terminal tagged protein was toxic to E. coli, resulting in
incomplete translated products. The C-terminal tagged NEK7-His6 had a much higher purity than
that of an N-terminal tag. The Ni2+ resin one-step purification led to a purity of 91.7%, meeting the
criteria of most kinase assays. With two-step and three-step procedures, the protein purities were
94.7% and ~100%, respectively. The NEK7 purified in this work maintained its kinase activity and
correct conformation, and the compound-protein interaction ability.

Conclusion:
Our optimized protocol could produce good purity of His tagged NEK7 in E. coli, and
the kinase activity and biophysical characteristics of which are preserved.
Copper Complexes as Antitumor Agents:In vitroandIn vivoEvidence
Authors: Balsa Lucia M. , Baran Enrique J. , León Ignacio E. 
Published: November 2021

:Copper is an essential element for most aerobic organisms, with an important function as a structural and catalytic cofactor, and in consequence, it is implicated in several biological actions. The relevant aspects of chemistry and biochemistry and the importance of copper compounds in medicine give us a comprehensive knowledge of the multifaceted applications of copper in physiology and physiopathology. In this review, we present an outline of the chemistry, and the antitumor properties of copper complexes on breast, colon, and lung cancer cells focus on the role of copper in cancer, the relationship between structure-activity, molecular targets, and the study of the mechanism of action involved in its anticancer activity. This overview is expected to contribute to understanding the design, synthesis, and uses of copper complexes as antitumor agents in the most common cancers.
Unfolded p53 as a Marker of Oxidative Stress in Mild Cognitive Impairment, Alzheimer’s and Parkinson’s Disease
Authors: García Sara , Amor-Gutiérrez Olaya , Palomares-Albarrán María , Toyos-Rodríguez Celia , Cuetos Fernando , Martínez Carmen , Costa-García Agustín , Fernández-Sánchez Maria Teresa , de la Escosura-Muñiz Alfredo , Novelli Antonello 
Published: November 2021

Aims:
There are several candidate biomarkers for AD and PD which differ in sensitivity,
specificity, cost-effectiveness, invasiveness, logistical and technical demands. This study is
aimed to test whether plasma concentration of unfolded p53 may help to discriminate among the
neurodegenerative processes occurring in Mild Cognitive Impairment, Alzheimer’s disease and
Parkinson’s disease.

Method:
An electrochemical immunosensor was used to measure unfolded p53 in plasma samples
of 20 Mild Cognitive Impairment (13 males/7 females; mean age 74.95±5.31), 20 Alzheimer’s (11
males/9 females; mean age: 77.25±7.79), 15 Parkinson’s disease patients (12 males/3 females;
mean age: 68.60 ± 7.36) and its respective age/sex/studies-matched controls.

Result:
We observed a significantly higher concentration of unfolded p53 in the plasma of patients
of each of the three pathologies with respect to their control groups (p=0.000). Furthermore,
the plasma concentration of unfolded p53 was significantly higher in Alzheimer’s disease patients
in comparison with Mild Cognitive Impairment patients (p=0.000) and Parkinson’s disease patients
(p=0.006). No significant difference between Mild Cognitive Impairment and Parkinson’s disease
patients was observed (p=0.524).

Conclusion:
Our results suggest that unfolded p53 concentration in the plasma may be a useful biomarker
for an undergoing neuropathological process that may be common, albeit with different intensity,
to different diseases.
Neuroprotective Effect of Fucoxanthin against Intracerebroventricular Streptozotocin (ICV-STZ) Induced Cognitive Impairment in Experimental Rats
Authors: Dhami Mahadev , Raj Khadga , Singh Shamsher 
Published: November 2021

Background:Alzheimer's disease (AD) is a neurological disorder characterized by loss of memory and cognitive functions caused by oxidative stress, neuroinflammation, change in neurotransmitter levels, and excessive deposition of Aβ(1-42)plaques. Fucoxanthin is a carotenoid with potential antioxidant, anti-inflammatory, and neuroprotective actions.Objective:In the present study, fucoxanthin was employed as a protective strategy in Intracerebroventricular Streptozotocin (ICV-STZ) induced experimental model of cognitive impairment.Methods:STZ was injected twice ICV (3 mg/kg) on alternate days 1 and 3, and Wistar rats were evaluated for the memory analysis using Morris water maze and elevated plus-maze. Fucoxanthin at low 50 mg/kg, p.o. and high dose 100 mg/kg, p.o. was administered for 14 days. All animals were sacrificed on day 29, and brain hippocampus tissue after isolation was used for biochemical (MDA, nitrite, GSH, SOD and Catalase), neuroinflammatory (TNF-α, IL-1β, and IL-6), neurotransmitters (ACh, GABA Glutamate), Aβ(1-42)and Tau protein measurements.Results:STZ-infused rats showed significant impairment in learning and memory, increased oxidative stress (MDA, nitrite), reduced antioxidant defense (GSH, SOD and Catalase), promoted cytokine release, and change in neurotransmitters level. However, fucoxanthin improved cognitive functions, restored antioxidant levels, reduced inflammatory markers dose-dependently, and restored neurotransmitters concentration.Conclusion:The finding of the current study suggests that fucoxanthin could be the promising compound for improving cognitive functions through antioxidant, anti-inflammatory, and neuroprotective mechanisms, and inhibition of acetylcholinesterase (AChE) enzyme activities, Aβ(1-42)accumulation, and tau protein.
Advancement and Strategies for the Development of Peptide-drug Conjugates:
Pharmacokinetic Modulation, Role and Clinical Evidence against
Cancer Management
Authors: Malviya Rishabha , Verma Swati , Sundram Sonali 
Published: November 2021

:
Currently, many new treatment strategies are being used for the management of cancer.
Among them, chemotherapy based on peptides has been of great interest due to the unique features
of peptides. This review discusses the role of peptide and peptides analogues in the treatment of
cancer, with special emphasis on their pharmacokinetic modulation and research progress. Low
molecular weight, targeted drug delivery, enhanced permeability, etc., of the peptide-linked drug
conjugates, lead to an increase in the effectiveness of cancer therapy. Various peptides have recently
been developed as drugs and vaccines with an altered pharmacokinetic parameter which has subsequently
been assessed in different phases of the clinical study. Peptides have made a great impact
in the area of cancer therapy and diagnosis. Targeted chemotherapy and drug delivery techniques
using peptides are emerging as excellent tools in minimizing problems with conventional chemotherapy.
It can be concluded that new advances in using peptides to treat different types of cancer
have been shown by different clinical studies indicating that peptides could be used as an ideal
therapeutic method in treating cancer due to the novel advantages of peptides. The development of
identifying and synthesizing novel peptides could provide a promising choice to patients with cancer.
Efficacy and Safety of Pidotimod in Childhood Wheezing: A Pilot Study
Authors: Manti Sara , Parisi Giuseppe Fabio , Papale Maria , Mollica Federico , Giugno Andrea , Leonardi Salvatore 
Published: November 2021

Background:
There is a growing need for effective therapies for the management of
wheezing in the pediatric population.

Aim:
We conducted a pilot, mono-centre, prospective, follow-up study to assess the efficacy and
the safety of Pidotimod (PDT) in the treatment of wheezing in children.

Methods:
Globally, 90 children (M:F=58:62, mean age 4.7±1.64 years) with recurrent viral wheezing
were enrolled in the study between October-November 2018. At baseline, children received
treatment with PDT as 1 vial of 400mg daily for 3 consecutive months. We evaluated the therapeutic
efficacy of PDT treatment at the end of 3 (T3) months of therapy as well as the long efficacy
and preventive efficacy of PDT treatment during a 3-months follow-up (T6) by using the following
outcomes: (i) How many patients showed one or more episodes of viral wheezing? (ii) How many
patients were taking concomitant medications (ICS, SABA, antibiotics)? (iii) How many patients
required ED visits? (iv) How many patients required hospitalization?

Results:
A significant decrease in the number of patients with at least one or more episodes of
wheezing and taking antibiotics was recorded after 3 months of treatment, and a further significant
decrease for both outcomes was reported at 3-months follow-up period (p<0.05). Differently, after
3 months of treatment, we found a significant decrease in the number of patients taking ICS and
SABA and in the number of patients requiring ED visits and/or hospitalization (p<0.05); however,
for all these outcomes, no further significant decrease was reported at follow-up period.

Conclusion:
We first showed that the administration of PDT is useful in the management of patients
with recurrent viral wheezing because we found a reduction in the number of patients requiring
ED visits and/or hospitalization as well as the number of patients taking drugs during the treatment
period. Moreover, to date, we found a long-term clinical effect over three months after treatment
suspension counteracting the recurrence of the disease.
Application of Silver Nanoparticles as a New Alternative Antiviral Agent
for SARS-CoV-2: A Review
Authors: Jamunkar Reena , Shrivas Kamlesh , Sinha Deepak , Patel Sanyukta , Monisha  , Patle Alka , Kumar Antresh , Banerjee Subhash 
Published: November 2021

Background:
Today, SARS-CoV-2 (COVID-19), a viral disease caused by the novel
coronavirus (a tiny crowned virus), has become one of the threats for human beings all over the
world and caused the death of millions of people worldwide. Many vaccines have been developed
and administered to people in several countries; however, due to their propensity to create new
strains, it appears that curing all corona strains will be challenging. So, it is necessary to identify the
structure of the virus, mechanism of action, and its antiviral activities against drugs and other functional
materials.

Method:
AgNPs have unique physicochemical and antimicrobial properties. This review describes
the structure and nature of the virus and the mechanism of action of an antiviral drug such as silver
nanoparticles (AgNPs) with the virus. In addition, different methods for synthesis of AgNPs, application
of AgNPs as an antiviral agent against influenza virus, human immuno deficiency virus
(HIV), herpes simplex virus type 1 (HSV-1), hepatitis B virus (HBV), polio virus, respiratory syncytial
virus (RSV), are discussed. Also, the most probable applications and properties of AgNPs that
can help prepare it as an antiviral agent are discussed.

Results:
The use of AgNPs against various viruses, including the coronavirus family, is found to be
effective; therefore, it can be considered for the development of antiviral agents, disinfectants, antiviral
coated mask, and their therapeutic use against the treatment of novel coronavirus with minimum
side effect and great efficiency.

Conclusions:
AgNPs were successfully used for the treatment of various viral diseases of the coronavirus
family such as H1N1, H3N2, influenza, even for SARS and MERS coronaviruses. AgNPs
coated masks, disinfectants, fabrics, wipes, and inhalation systems are effective for the inhibition of
SARS-CoV-2 infection. Since sanitizers have a temporary effect, the development of some other
potential alternatives having low toxicity, ease of use, long lasting efficiency, health cautiousness,
minimum side effect, sustainable fabrics is required.
Acceptability of an mHealth App for Youth With Substance Use and Mental Health Needs: Iterative, Mixed Methods Design
Authors: Adams Zachary , Grant Miyah , Hupp Samantha , Scott Taylor , Feagans Amanda , Phillips Meredith Lois , Bixler Kristina , Nallam Phani Teja , La Putt Dorothy 
Published: November 2021

Background
Treating substance use disorders (SUDs) during adolescence can prevent adult addiction and improve youth outcomes. However, it can be challenging to keep adolescents with SUDs engaged in ongoing services, thus limiting potential benefits. Developmentally appropriate tools are needed to improve treatment engagement during and between sessions for youth with SUDs and mental health disorders. Mobile health apps may augment or replace psychotherapy components; however, few have been developed specifically for youth with SUDs following user-guided design principles, which may limit their appropriateness and utility. Formative research on acceptability to intended end users is needed before the efficacy of such tools can be examined.

Objective
This study involves user-centered, iterative development and initial user testing of a web-based app for adolescents with SUDs and mental health concerns.

Methods
Adolescents aged 14 to 17 years with past-year involvement in outpatient psychotherapy and behavioral health clinicians with adolescent SUD treatment caseloads were recruited. Across 2 assessment phases, 40 participants (alpha: 10 youths and 10 clinicians; beta: 10 youths and 10 clinicians) viewed an app demonstration and completed semistructured interviews and questionnaires about app content and functionality.

Results
Participants expressed positive impressions of the app and its potential utility in augmenting outpatient therapy for youth with SUDs and mental health concerns. Noted strengths included valuable educational content, useful embedded resources, and a variety of activities. Adolescents and clinicians favored the app over conventional (paper-and-pencil) modalities, citing convenience and familiarity. The app was found to be user-friendly and likely to improve treatment engagement. Adolescents suggested the inclusion of privacy settings, and clinicians recommended more detailed instructions and simplified language.

Conclusions
The novel app developed here appears to be a promising, acceptable, and highly scalable resource to support adolescents with SUDs and mental health concerns. Future studies should test the efficacy of such apps in enhancing adolescent behavioral health treatment engagement and outcomes.
Are eSports more than just sitting? A study comparing energy expenditure
Authors: Kocak Umut Ziya 
Published: November 2021

Aim:
To investigate the energy expenditure of amateur eSport athletes between sitting and an eSport gaming session.
Materials & methods:
Eleven amateur male eSport athletes (age: 21.73 ± 3.50 years, weight: 76.00 ± 12.51 kg, height: 1.81 ± 0.06 m) were included. After recording the resting values, eSports playing values were recorded using an open circulation oxygen consumption analyzer (QuarkCPET, COSMED, Albano Laziale, Italy). Also, action per min (APM) scores were recorded.
Results:
When comparing the respiratory frequency, metabolic equivalent of task values and energy expenditure values of the participants, a significant increase was observed in all three values (p < 0.05 for each).
Conclusion:
eSports players, who are considered to be physically inactive by sitting, spend approximately 40% more energy than sitting, even at the amateur level. According to APM, more energy can be spent in tournaments and at the elite level.
The Perception of Risk Disclosure Characteristics on the Credit Default Swap Market—An Automated Analysis
Authors: Nagel Deborah Yvonne , Fuhrmann Stephan , Tietmeyer Raphael , Guenther Thomas W. 
Published: November 2021

SYNOPSIS
This paper evaluates the associations between credit default swap (CDS) spreads and risk disclosure characteristics, especially the expected qualitative and the expected quantitative impacts of risks on companies' future performance and information on risk management. We find that CDS investors can benefit from information on expected risk impacts and from information on risk management, which is important for the current discussion of the Securities and Exchange Commission (SEC) on risk disclosure regulation. However, for companies, the disclosure of such information can be either beneficial or costly, depending on the initial risk perception of CDS investors prior to the publication of risk disclosures and on the disclosed risk factors. Furthermore, we expand the literature by automatically measuring the mentioned risk disclosure characteristics using dictionary-based approaches.
Factors that Influence the Work Interest of Prospective Undergraduate Education in Industry
Authors: Widiyantoro Agung , Sudira Putu , Rabiman Rabiman , Tuah Yayan Adrianova Eka 
Published: November 2021

This study aims to analyze the influence of working interest in the Mechanical Engineering Education (MEE) student industry on income, attention, willingness, family environment, and community environment; the influence of working interest in the MEE student industry on income expectations; and the influence of working interest in the MEE student industry on attention factors; the influence of working interest in the MEE student industry on the willingness factor; the influence of working interest in the MEE student industry on family environmental factors, and the influence of working interest in the MEE student industry on community environmental factors. This study uses the ex-post-facto method. The study included 82 undergraduate students. Simple random sampling was utilized as the sampling technique with a questionnaire to collect the data collection. The findings revealed that: income expectations, attention, willingness, family environment, and community environment all had a positive and significant influence on working interest in the industry; income expectations had a positive and significant influence on working interest in the industry; attention has a positive and significant influence on working interest in industry, willingness has a positive and significant influence on working interest in the industry, family environment has a positive and significant influence on working interest in the industry, and community environment has a positive and significant influence on working interest in the industry.
Usability Testing SIKTA di Program Studi Manajemen Informatika dari Pengguna Mahasiswa
Authors: Marti Ni Wayan , Surya Mahedy Kadek 
Published: November 2021

Penelitian ini bertujuan untuk melakukan usability testing terhadap Sistem Informasi Kontrol Mutu dan Pengelolaan Tugas Akhir Mahasiswa di Program Studi Manajemen Informatika (SIKTA). SIKTA merupakan sistem informasi yang digunakan untuk melakukan kontrol terhadap proses penyelesaian Tugas Akhir Mahasiswa. SIKTA adalah aplikasi yang digunakan secara internal di Program Studi Manajemen Informatika. Metode yang digunakan untuk melakukan usability testing terhadap SIKTA adalah ISO 9241-11. ISO 9241-11 merupakan uji usability untuk mengukur tingkat efektivitas, efisiensi dan kepuasan pengguna. Tingkat efektivitas dan efisiensi diukur dengan pemberian tugas-tugas kepada responden, sedangkan untuk mengukur kepuasan pengguna digunakan kuisioner dari System Usability Scale (SUS). Dari hasil uji diperoleh masing-masing yaitu uji keefektifan sebesar 92%, uji keefisienan memperoleh 88% dan kepuasan pengguna menggunakan SUS memperoleh angka 76,5. Dari uji tersebut, dapat dikatakan bahwa SIKTA merupakan sistem yang efektif, efisien dari sisi mahasiswa sebagai penggunanya. Dan angka 76,5 menyatakan bahwa pengguna puas dalam menggunakan SIKTA.
PENGEMBANGAN MEDIA PEMBELAJARAN ANIMASI 2D PADA MATA PELAJARAN FISIKA KELAS X DI SMA NEGERI 1 SAWAN
Authors: Wahyuni Ni Luh Dinda Ajeng , Sugihartini Nyoman , Sindu I Gede Partha 
Published: November 2021

Pengembangan media pembelajaran sangat diperlukan pada mata pelajaran fisika di SMA Negeri 1 Sawan. Hal tersebut dikarenakan dalam proses pembelajaran guru masih menggunakan metode ceramah. Sekolah menyediakan buku paket pelajaran sebagai sumber belajar pokok dan lembar kerja siswa (LKS) sebagai penunjang proses pembelajaran. Penggunaan media sebagai pembelajaran masih terbatas pada media power point dan video yang diambil di internet. Untuk mengatasi masalah ini, dikembangkan media pembelajaran animasi 2D untuk kelas X untuk memfasilitasi proses belajar mengajar dan memotivasi siswa dalam suasana pembelajaran menjadi lebih menyenangkan. Metode yang digunakan dalam penelitian ini adalah penelitian dan pengembangan (research and development), untuk mengembangkan media pembelajaran animasi 2D pada mata pelajaran fisika. Model yang akan digunakan adalah model Multimedia Development Life Cycle, yang memiliki 6 tahapan yaitu concept, design, material collecting, assembly, testing dan distribution. Hasil penelitian ini berupa sebuah media pembelajaran yang baik dikemas dalam bentuk CD. Terdapat beberapa pengujian untuk mengetahui kelayakan media, terdiri uji ahli isi dengan hasil 100%, uji ahli media 100%, uji efektifitas 74,73% termasuk dalam kriteria tinggi, uji respon guru 88% dan uji respon siswa diperoleh 81,02% termasuk dalam kriteria baik.