bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Effect of Glycerol as a Cryoprotective Agent in the Cryopreservation of Adipose Tissue
Authors: Zhang Pei-Qi , Tan Poh-Ching , Gao Yi-Ming , Zhang Xiao-Jie , Xie Yun , Zheng Dan-Ning , Zhou Shuang-Bai , Li Qing-feng 
Published: March 2021

Abstract
Background: Long-term preservation of adipose tissue is crucial for clinical applications. Researchers should consider both efficiency and biosafety when choosing a cryoprotective agent (CPA) for adipose tissue preservation. Glycerol has been applied as a nontoxic CPA for multiple tissues but not adipose tissue. We aimed to evaluate the efficacy of glycerol as a CPA for adipose tissue cryopreservation.Methods: Fresh human adipose tissues were obtained from ten patients who underwent liposuction and divided into 1 ml samples. Each sample was randomly mixed with 1 ml of CPA: 60 to 100% glycerol, 0.25 mol/L trehalose or DMSO+FBS and cryopreserved in -196 °C liquid nitrogen for one month. After thawing and elution, the tissues were immediately evaluated for activity and structural integrity in vitro. Then, 0.2 ml of each sample was transplanted subdermally to the nude mouse dorsum and harvested after one month for histological examination to assess the effect of the cryopreserved fat in transplantation.Results: After cryopreservation, the samples treated with DMSO+FBS, trehalose, and 60% and 70% glycerol had a more integrated structure than the samples in other groups. Tissues preserved with 70% glycerol had the highest tissue activity, close to that of fresh tissues. Adipose-derived stem cells (ADSC) viability, proliferation and differentiation capability were also better in 70% glycerol group. In vivo analysis showed that tissue preserved with 70% glycerol had superior retention rates and structural integrity. Compared to the DMSO+FBS and trehalose groups, the glycerol group showed lower inflammation.Conclusion: Glycerol (70%) is efficient in adipose tissue cryopreservation. Glycerol-based CPAs, which are nontoxic and show biosafety, are a promising solution for clinical tissue cryopreservation.
The Significance of Neural Inter-Frequency Correlations
Authors: Savolainen Oscar Wiljam 
Published: March 2021

Abstract
It is of great interest in neuroscience to determine what frequency bands in the brain contain common information. However, to date, a comprehensive statistical approach to this question has been lacking. As such, this work presents a novel statistical significance test for correlated power across frequency bands in non-stationary time series. The test accounts for biases that often go untreated in time-frequency analysis, i.e. intra-frequency autocorrelation, inter-frequency non-dyadicity, and multiple testing under dependency. It is used to test all of the inter-frequency correlations between 0.2 and 8500 Hz in continuous intracortical extracellular neural recordings, using a very large, publicly available dataset. The results show that neural processes have signatures across a very broad range of frequency bands. In particular, LFP frequency bands as low as 20 Hz were found to almost always be significantly correlated to kHz frequency ranges. This test also has applications in a broad range of fields, e.g. biological signal processing, economics, finance, climatology, etc. It is useful whenever one wants to robustly determine whether short-term components in a signal are robustly related to long-term trends, or what frequencies contain common information.
Predictability of Sinusoidal Stimulus Movement in Accommodative Demand Does Not Improve Accommodation
Authors: Águila-Carrasco Antonio J. Del , Marín-Franch Iván 
Published: March 2021

Abstract
Previous research work suggests that predictable target motion such as sinusoidal movement can be anticipated by the visual system, thereby improving the accommodative response. The validity of predictable motion for studying human dynamic accommodation is sometimes put into question. The aim of this work was to assess from a practical perspective the effect of anticipation along with learning (and motivation, etc.) and fatigue (and boredom, loss of attention, etc.) on dynamic accommodation experiments. Specifically, changes in amplitude and temporal phase were estimated within and between trials as 9 adult observers were instructed to focus on a stimulus that oscillated in distance at specific temporal frequencies. On average, amplitude decreased whereas phase increased within trials. No evidence of anticipation or learning was observed either within or between trials. Fatigue consistently dominated anticipation and learning within the course of each trial. Even if the eye is equipped by a “prediction operator”, in practice, it is fatigue, and not anticipation or learning, that seems to muddle the results from dynamic accommodation experiments.
Numerical Investigation on the Effects of Circuit Parameters on the Plastic Deformation of Fastener Holes in Thin Aluminum Alloy via Electromagnetic Expansion Process
Authors: Xu Xiaofei , Geng Huihui , Cao Qingshan , Cao Quanliang , Li Liang , Ouyang Xiaoping 
Published: March 2021

Abstract
The size of plastic deformation zone during fastener hole strengthening is a critical indicator of the strengthening effect. In this study, a considerable plastic deformation zone in 1.5 mm aluminum alloy plate with a hole was produced via electromagnetic strengthening. The finite element analysis results showed that the electromagnetic strengthening process could achieve high compressive hoop residual stress around the fastener hole in thin plate without serious axial deformation compared with conventional cold hole expansion process. The simulation results were experimentally validated by the grid method. Furthermore, for the same discharge energy, the size of plastic deformation zone varies with the discharge capacitance, and there was an optimal combination of the discharge capacitance and discharge voltage. What’s more, even the plastic deformation zone was the same at the maxed load, different unloading process during the electromagnetic hole expansion process also had a great influence on the strengthening effect.
NORHA, A Novel Follicular Atresia-related Lncrna, Promotes Granulosa Cell Apoptosis via miR-183-96-182 Cluster and FoxO1&nbsp;Axis
Authors: Yao Wang , Pan Zengxiang , Du Xing , Zhang Jinbi , Liu Honglin , Li Qifa 
Published: March 2021

Abstract
BackgroundFollicular atresia has been shown to be strongly associated with low follicle utilization rate and female infertility, which are regulated by many factors such as miroRNAs (miRNAs), a class of non-coding RNAs (ncRNAs). However, little is known about long non-coding RNAs (lncRNAs), another ncRNAs, which regulate follicular atresia. ResultsA total of 94 differentially expressed lncRNAs, including 74 up-regulated and 20 down-regulated lncRNAs, were identified in early atretic follicles compared to healthy follicles by RNA-sequencing. We identified and characterized a non-coding RNA that was highly expressed in atretic follicles (NORHA), an intergenic lncRNA, was the most significantly elevated lncRNA in early atretic follicles. Functionally, RT-PCR，flow cytometry and western blot results showed that NORHA was associated with follicular atresia by influencing GC apoptosis. Mechanistically, bioinformatics analysis, luciferase reporter assay and RNA immunoprecipitation assay results showed that NORHA acted as a ‘sponge’, which directly bound to the miR-183-96-182 cluster, and therefore resisted their targeting inhibition of FoxO1, the major sensor and effector of oxidative stress. Furthermore, NORHA and oxidative stress synergistically induced GC apoptosis. ConclusionsWe provide a comprehensive perspective of lncRNAs regulation of follicular atresia, and demonstrate that NORHA, a novel lncRNA related to follicular atresia, induces GC apoptosis through affecting the miR-183-96-182 cluster and Foxo1 axis
Solitary Bone Plasmacytoma: The Unusual Case with Involvement of Adjacent Disc Space
Authors: Tan Hong-dong , Gu Jia , Xu Liang , Sun Gang 
Published: March 2021

Abstract
Background: Solitary plasmacytoma of the bone (SPB) is a rare manifestation of plasma cell tumor that usually presents as a osteolytic lesion mainly localized within the axial skeleton and the back pain is a common clinical feature.Case presentation: This case report discussed a 57-year-old male presented with low back pain caused by SBP. In this case, there is an even rarer phenomenon presented as osteolytic destruction in T7 to T9 vertebral bodies with involvement of adjacent disc spaces. This case report provided a comprehensive description of the radiographic assessment, medical management, and differential diagnosis.Conclusions: This is the first discussion focusing on differential diagnosis between spinal neoplasm and infectious diseases about SBP with involvement of adjacent disc space.
Silencing Herg1 by shRNA inhibits the proliferation and invasion of osteosarcoma in vivo and in vitro
Authors: Chen Zhida , Song Chao , Chen Yunping , Xiao Lili , Jiang Yuanjie , Wu Jin 
Published: March 2021

Abstract
Background: Osteosarcoma is the most common malignant bone tumor of childhood, and improvements in the survival rates have reached a plateau phase. This study was aimed to explore the the importance of the human ether-a-go-go-related potassium channel (Herg1) in the proliferation and invasion of osteosarcoma.Methods: The effects of Herg1 silenced on osteosarcoma cell proliferation and invasion were detected by were detected by CCK-8 assay, wound healing assay and transwell assay, respectively. Tandem affinity purification, mass spectrometry and Dual luciferase reporter assay were used to find out possible molecules responsible for the action of Herg1 on osteosarcoma cells. Nude mouse model was used to investigate the in vivo functions of Herg1. Results: Herg1 silencing by shRNA significantly suppressed the proliferation and invasion of osteosarcoma cells in vitro as well as tumorigenicity and metastasis in nude mice. Moreover, Herg1 promoted osteosarcoma progression via turning off Hippo signaling pathway, leading to the activation of Yes-associated protein (YAP). Mechanistically, Herg1 co-localized and interacted with NF2 (also called Merlin), and this interaction probably caused the de-phosphorylation of MST1/2 and LATS1/2, which drove YAP nuclear translocation and transcriptional activation. Conclusion: Herg1 acts as an oncogene in osteosarcoma and may therefore serve as a useful therapeutic target for osteosarcoma patients.
Users’ Perception on Factors Contributing to Electronic Medical Records Systems Use: A Focus Group Discussion Study in Healthcare Facilities Setting in Kenya
Authors: Ngugi Philomena N. , Babic Ankica , Were Martin C. 
Published: March 2021

Abstract
Background Electronic Medical Records systems (EMRs) adoption in healthcare to facilitate work processes have become common in many countries. Although EMRs are associated with quality patient care, patient safety and cost reduction, their adoption rates are comparatively low. Understanding factors associated with usage of implemented EMRs are critical for advancing successful implementations and scale-up sustainable initiatives. The aim of this study was to explore end users’ perceptions and experiences on factors facilitating and hindering EMRs use in healthcare facilities in Kenya. Methods Focus group discussions were conducted with EMRs users (n=20) each representing a healthcare facility determined by the performance of the EMRs implementation. Content analysis was performed on the transcribed data and relevant themes derived.Results Six thematic categories for both facilitators and barriers emerged, and these related to: (1) system functionalities; (2) training; (3) technical support; (4) human factors; (5) infrastructure, and (6) EMR operation mode. The identified facilitators included: easiness of use and learning of the system, efficiency of EMRs in patient data management, responsiveness of Information Technical (IT) support, user training on EMR system upgrades. The identified barriers included: frequent power blackouts, inadequate computers, retrospective data entry (RDE) EMRs operation mode, lack of continuous training on system upgrades, and delayed IT support. Conclusion Users in an LMIC setting generally believed that the EMRs improved work process, with multiple identified facilitators and barriers to their use. Most users perceived system functionality and training as motivators to EMRs use, while infrastructure challenge was the greatest barrier.
The risk factors of surgical site infection following posterior cervical spine surgery
Authors: Chang Yvang , Yang Ming , Zhang Wentao , Xu Gang , Li Zhonghai 
Published: March 2021

Abstract
Background: Surgical site infection (SSI) is a common complication following posterior cervical spine surgery, imposing a high burden on patients and society. However, information about its characteristics and related risk factors is limited. We designed this study intended to address this issue.Methods: From January 2011 through October 2020, a total of 405 patients diagnosed of cervical degenerative diseases (cervical spondylotic myelopathy, ossification of posterior longitudinal ligament and cervical disk herniation) who were treated with unilateral open-door lamnioplasty surgeries were enrolled in this study. We divided the patients into the SSI group and the non-SSI group and compared their patient-specific and procedure-specific factors. Univariate and multiple logistic regression analyses were performed to determine risk factors. Results: There were significant differences between groups in subcutaneous fat thickness (FT) (P<0.001), ratio of subcutaneous FT to muscle thickness (MT) (P<0.001), preoperative Japanese Orthopaedic Association（JOA）Scores (P< 0.003), preoperative serum albumin (P< 0.001), postoperative drainage (P<0.004), time of draining (P<0.001). Logistic regression analysis of these differences showed that ratio of subcutaneous FT/MT, preoperative JOA score, preoperative serum albumin and longer time of draining were significantly related to SSI (P<0.05).Conclusion: Ratio of subcutaneous FT/MT, preoperative JOA score, preoperative serum albumin and longer time of draining are identified as the independent risk factors of SSI in posterior cervical spine surgeries. Identification of these risk factors could be useful in reducing SSI incidence and patients counseling.
Laser-triggered combined chemotherapy and photodynamic therapy enabled by iron sulfide-doxorubicin@bovine lactoferrin nanozymes for breast cancer therapy
Authors: Bai Qian 
Published: March 2021

Abstract
BackgroundThe use of magnetic nanozymes with the ability to synchronize gas therapy through photodynamic and chemotherapy in the treatment of breast cancer has received much attention. ResultsHence, in this study, we designed a bovine lactoferrin-coated iron sulfide nanozymes containing doxorubicin (FeS-Dox@bLf NZs) by wet chemical synthesis that can respond to tumor acidity. Then, the physicochemical properties of synthesized NZs were investigated by TEM, SEM, DLS and spectroscopic methods. Likewise, the level of Fe2+ release, H2S and Dox from FeS-Dox@Lf NZs under acidic conditions was evaluated. It was observed that Fe2+ and S2- caused significant OH and H2S release through the Fenton reaction. Also, the toxic effects of FeS-Dox@Lf NZs on 4T1 cancerous cells were investigated by MTT and flow cytometry assays. After intravenous injections of NZs and laser irradiation, significant effects of FeS-Dox@Lf NZs on mice weight and tumor status were observed. Afterwards, not only the distribution of Dox in the body was examined by fluorescent, but also the time of Fe clearance and the amount of Dox and Fe retention in vital tissues were determined. The findings confirm that FeS-Dox@Lf NZs, in addition to targeted drug distribution in tumor tissue, resulted in superior therapeutic performance compared to free Dox due to reduced Dox side effects in vital tissues, and increased level of free radicals in 4T1 cells. ConclusionOverall, FeS-Dox@Lf nanozymes with the ability to synchronize chemotherapy and gas therapy raised hopes for more effective treatment of breast cancer.
Corydalis Saxicola Bunting Total Alkaloids Inhibits Paclitaxel-Induced Peripheral Neuropathy By Regulating PKCε-TRPV1 and p38 MAPK-TRPV1 Signaling Pathways
Authors: xue chu , Liu Si-Xue , Hu Jie , Huang Jin , Liu Hong-Min , Qiu Zhi-Xia , huang fang 
Published: March 2021

Abstract
Background: Corydalis saxicola Bunting, a traditional Chinese medicine, has been proven to work well in anti-inflammation, blood circulation improvement, hemostasis, analgesia. This study was designed to observe the effects and potential mechanism of Corydalis saxicola Bunting total alkaloids (CSBTA) on paclitaxel-induced peripheral neuropathy (PIPN). Materials and methods: Following 4 times intraperitoneal injections of paclitaxel (2 mg/kg) and intragastrically (i.g.) administrated at 30 or 120 mg/kg CSBTA, mechanical and thermal allodynia and hyperalgesia in rats were tested. After 40 days, serum was collected for the detection of PGE2, TNF-α, and IL-1β by ELISA. The L4-L6 segment spinal cord, DRG, and plantar skin were harvested, and protein and gene expression of CGRP, SP, TRPV1, p38, and PKCε were analyzed by Western-blot or RT-qPCR. Parallelly, the PIPN cell model was also established in primary DRG neurons by paclitaxel stimulation (300 nM, 5 d). We examined PGE2, TNF-α and CGRP mRNA levels, and the protein expression on the PKCε-TRPV1 and p38 MAPK-TRPV1 pathways in PIPN cell model with or without CSBTA (25 μg/ml and 50 μg/ml). Results: The results showed that CSBTA effectively ameliorated allodynia and hyperalgesia in PIPN rats, regulated the contents of cytokines and neuropeptides in different tissues and cell models. CSBTA significantly decreased the protein expression of PKCε-TRPV1 and p38 MAPK-TRPV1 signaling pathways in the spinal cord and DRG tissues in the PIPN animal model and primary DRG neurons. Conclusion: Therefore, CSBTA has a perspective therapeutic effect on the treatment of paclitaxel-induced peripheral neuropathy.
Effects of a Snack on Performance and Errors During a Simulated 16-h Night Shift: a Pilot Study
Authors: Oriyama Sanae , Yamashita Kotomi 
Published: March 2021

Abstract
Background: Night shift workers might not eat due to their busy schedules during the night shift. However, diet may not only satisfy hunger, but also affect performance and errors.The aim of this study was to clarify the effect of a snack on performance and errors during 2-day, 16-h simulated night shifts. Methods: A randomized repeated-measure crossover study was performed to investigate subjective and cognitive performance in 15 healthy female adults (mean age, 21.7 years) after they consumed a snack (352 kcal) during a simulated night shift (16:00 to 09:00). The participants were kept awake from wake up in the morning to the next day at 09:00. Subjects were tested for performance on the Uchida-Kraepelin test, as well as for subjective feeling, body temperature, psychomotor vigilance test, and heart rate variability, before and after they consumed the snack. One day before the experiment, all participants wore an actigraphy monitoring device to determine their sleep state. Results: There was no difference between the snack condition and the skipping condition in sleep states the day before the experiment. On the day of the experiment, between 16:00 and 09:00, subjective sleepiness, fatigue, and body temperature were not different between the two conditions. Subjects showed a significant improvement in performance on the Uchida-Kraepelin test and total errors on the psychomotor vigilance test, the primary outcome measure, when they consumed a snack compared with the skipping condition. In addition, the snack condition was associated with decreased high-frequency power, decreased low-frequency power/high-frequency power ratio, and increased heart rate, in the vagally mediated heart rate variability indices, which may reflect a higher ability to modulate cognitive and behavioral processes. Conclusions: These results suggest that providing a snack to shift workers during night shifts might improve work safety and efficiency.Trial registration: This study was registered with the University Hospital Medical Information Network-Clinical Trials Registry (UMIN-CRT registry ID: UMIN 000034345).
A Novel Method of Defect Detection Based on Experimental Investigation of Galvanized Steel Welded Plates Using Wobble Laser Process
Authors: Karganroudi Sasan Sattarpanah , Aminzadeh Ahmad , Kemda Vincent Blériot Feujofack , Barka Noureddine 
Published: March 2021

Abstract
This paper aims at investigating the effect of laser welding parameters on the hardness profile, using hardness mapping analyses, and welding geometry of galvanized steel plates. Hardness distribution and geometry deflection of galvanized welded thin plates are commonly applied in fields where the weld quality is of utmost importance. Due to the welding process and material condition, welding galvanized steel is one of the problematic matters in welding technology. Here, the design of experiment (DOE) approach is used to study the effect of process parameters. Using a pattern matrix of micro-indentation hardness experiment, the welding defects are visualized on hardness profile of the weld cross-section. The effect of process parameters on welding defect formation is then qualitatively analyzed. The geometrical defects of welding such as weld width and voids are then quantitatively studied based on analysis of variance (ANOVA), and predictive models of welding voids and weld seam width are developed based on the regression method. Response surface method (RSM) is then applied to define the trend of process factors interaction on the welding defects. The experimental results confirm the reliability of developed predictive models of welding defects geometry, weld width, and voids area of laser-welded galvanized plates.
Investigation of the Wear Behavior of Abrasive Grits in a Dry Machining Inconel 718 Narrow-Deep-Groove with a Single-Layer Cubic Boron Nitride Grinding Wheel
Authors: Li Guang , Liang Guoxing , Shen Xingquan , Lv Ming , Liu Donggang , Hao Xinhui , Huang Yonggui , Princewill Tochukwu Ojiako , Al-Nehari Mohammed 
Published: March 2021

Abstract
The wear behavior of a single-layer electroplated cubic boron nitride (cBN) grinding wheel was investigated in creep feed profile grinding Inconel 718 narrow-deep-groove with a width of 2 mm. In this paper, the protrusion height of cBN grits on the side edge of the grinding wheel was measured by three-dimensional optical profiler, and a scanning electron microscope was used to detect the topography of worn cBN grit and the nickel bonding layer. The investigation results indicated that the steady wear stage of the grinding wheel shared more than 84.6% of the wheel lifespan, and the machining precision of the grinding wheel was kept at a high level. The fracture mode of the cBN grit was proven to be cleavage. The source of the cleavage cracks was observed on the surface of wear flat or the side surface of the cBN grit. The holding strength of cBN grit could be weakened due to the joint surface breakage, the displacement of grit, or the cracks of the nickel bonding layer. The transition layer was contributed to the decrease of the bonding strength. Severe macro fractures and the concentration of pulled out grits were observed on the transition edge and the inner area of the side edge, separately. This study provides a deep understanding of the wear mechanism of a single-layer electroplated cBN grinding wheel.