bluuebunny/crossref_metadata_2025_split huggingface dataset:
Dioscorea Zingiberensis New Saponin Inhibits the Growth of Hepatocellular Carcinoma by Suppressing the Expression of Long Non-coding RNA TCONS-00026762
Authors: Liu Xing , Zhou Pingsheng , He Keqing , Wen Zhili , Gao Yong 
Published: May 2021

Background: The etiology and carcinogenesis of hepatocellular carcinoma (HCC) are associated with various risk factors. Saponins extracted from Dioscorea zingiberensis C. H. Wright exhibit antitumor activity against HCC. This study aimed to investigate the effect and the underlying mechanism of Dioscorea Zingiberensis new saponin (ZnS) on HCC.Methods: Human HCC cell lines, Huh7 and SMMC-7721, were treated with different concentrations of ZnS. Cell apoptosis was determined via flow cytometry assay. Differentially expressed lncRNAs (DElncRNAs) in ZnS-treated SMMC-7721 cells were determined through RNA-sequence. The role of lncRNA TCONS-00026762 in HCC was investigated gain of function analysis, along with cell proliferation, apoptosis, and invasion in HCC cells. A subcutaneous xenograft of SMMC-7721 cell lines was established to study the effects of TCONS-00026762 in vivo. The expression of apoptosis-related proteins was detected in vivo and in vitro via western blotting.Results: ZnS inhibited the proliferation of HCC cell in a dose-dependent manner. ZnS could induce apoptosis in HCC cells. Illumina sequencing results showed that 493 DElncRNAs were identified in ZnS-treated SMMC-7721 cells. TCONS-00026762 expression was down-regulated in the ZnS-treated SMMC-7721 cells. TCONS-00026762 inhibited the effect of ZnS on the proliferation, apoptosis, and invasion of HCC cells. ZnS inhibited the tumor growth, while, TCONS-00026762 promoted tumor growth in vivo. Furthermore, ZnS and TCONS-00026762 regulated cell apoptotic pathways.Conclusion: ZnS significantly inhibits the viability, apoptosis, invasion, and tumorigenicity of HCC cells by regulating the expression of TCONS-00026,762. Our findings provide novel insights into the potential role of lncRNA in HCC therapy.
Couples Coping With the Serious Illness of One of the Partners
Authors: Riazuelo Hélène 
Published: April 2021

Chronic kidney failure is a serious somatic disease. Addressing the issue of living with a chronic disease means fully considering the patients’ entourage, their families, and those close to them, especially their children and spouses.Objectives: The present paper focuses on the couple’s psychological experience when one of them suffers from a chronic disease, in this instance kidney disease. In particular, how is the spouse affected by the treatment provided? The aim is not only to see how care for sick people can be improved, but also, more specifically, how relatives and especially partners can receive attention.Methodology: A qualitative approach is not only adopted, being based on the psychotherapeutic follow-up of the partners of patients with chronic kidney disease, but also of the patients themselves, addressing the matter of their life as a couple. Three couples were considered, and two case studies are presented here. The issues that cut across these different situations are examined.Results and Discussion: Some couples show considerable resourcefulness. However, over the years, that capacity for adaptation and inventiveness can also be interrupted by the periods of greater suffering and even despair, especially when the somatic pathology becomes chronic. Many spouses talk about how living with a sick partner weighs down on them, causing severe fatigue. Some aspects of the illness can also become traumatic. The disease regularly disrupts the daily life of the couple and the family. This leads to a reworking of family relations. Each couple has its own history with the condition. As it emerges, it can disrupt the bonds of filiation, especially when the illness is hereditary. Making psychological care more accessible to the partners involved constitutes a major challenge for our hospital care systems.
Depression and Suicidal Ideation in a Sample of Malaysian Healthcare Workers: A Preliminary Study During the COVID-19 Pandemic
Authors: Sahimi Hajar Mohd Salleh , Mohd Daud Tuti Iryani , Chan Lai Fong , Shah Shamsul Azhar , Rahman Farynna Hana Ab , Nik Jaafar Nik Ruzyanei 
Published: April 2021

Objective: The burden of suicidal behavior is anticipated to increase as a sequela of the COVID-19 pandemic. However, there is limited evidence on suicidal behavior among healthcare workers, an at-risk population. Our study aimed to investigate suicidal ideation in terms of the rate and associated factors in a sample of Malaysian healthcare workers during the early-phase of the COVID-19 pandemic.Methods: A subpopulation analysis (N = 171) was conducted within a larger, nation-wide cross-sectional study of Malaysian healthcare worker psychological distress from March 18–21, 2020. Current suicidal ideation was measured with item 9 of the Patient Health Questionnaire-9 (PHQ-9). The following independent variables were assessed: socio-demographic profile, occupation and service-related factors, health-anxiety (Health Anxiety Inventory, HAI), lifetime anxiety disorder and severity of depression (PHQ-9).Results: The proportion of healthcare workers with current suicidal ideation (19/171) and clinical depression (17/171) were 11.1 and 9.9%, respectively. Multivariable analysis showed that clinical depression was the most significant factor associated with current suicidal ideation (p &lt; 0.001, OR = 55.983, 95% CI = 9.015–347.671) followed by mild (subthreshold) depression (p = 0.001, OR = 115.984, 95% CI = 2.977–85.804). Service duration of more than 10 years was associated with significantly less suicidal ideation (p = 0.049, OR = 0.072, 95% CI = 0.005–0.993).Conclusions: Depression (subthreshold and especially within the clinical range) and early-career status (&lt;10 years in service) may be target areas of early intervention for reduction of suicidal ideation amongst healthcare workers who have served during the COVID-19 pandemic. Further research is warranted to elucidate specific occupational stressors related to COVID-19 work conditions to tailor appropriate suicide preventive strategies in this population.
Perceived Social Norms as Determinants of Adherence to Public Health Measures Related to COVID-19 in Bali, Indonesia
Authors: Indrayathi Putu Ayu , Januraga Pande Putu , Pradnyani Putu Erma , Gesesew Hailay Abrha , Ward Paul Russel 
Published: April 2021

Introduction: Before the widespread availability of an effective COVID-19 vaccine, it is crucial to control the rate of transmission by ensuring adherence to behavioral modifications, such as wearing masks, physical distancing, and washing hands, all of which can be implemented as public health measures. Focusing on the conditions in Bali, this study explored the level of compliance to public health measures targeted at COVID-19 and identified the determinants of compliance via the values, rules, and knowledge approach.Materials and Methods: This cross-sectional study conducted an online survey using the Google Form application from June 29 to July 5, 2020. The minimum required sample size was 664. Inclusion criteria were set as follows: 18 years of age or older and residing in Bali during the data collection period. Adherence was measured based on nine protocol indicators that were rated using a four-point Likert scale. A multiple linear regression analysis was then conducted to determine the associated factors of adherence to public health measures.Results: Of the 954 survey respondents, data from 743 were included for analysis. The average level of adherence to public health measures was 32.59 (range of 20–36). The linear regression analysis showed that perceived health benefits from public health measures, being female, and having COVID-19 test histories were significantly associated with adherence to public health measures.Conclusions: For public health measures targeted at COVID-19, adherence was strongly associated with perceived social norms, in which individuals played social community roles by adapting to standardized public health measures. It is thus imperative for governments to support and monitor public health measures during the COVID-19 pandemic.
The Prevalence of Metabolic Disease Multimorbidity and Its Associations With Spending and Health Outcomes in Middle-Aged and Elderly Chinese Adults
Authors: Zhao Yang , Zhang Puhong , Lee John Tayu , Oldenburg Brian , Heusden Alexander van , Haregu Tilahun Nigatu , Wang Haipeng 
Published: May 2021

Objective: Metabolic diseases have been a clinical challenge worldwide and a major public health issue. Very few studies from China investigated the impact of metabolic multimorbidity on healthcare and health outcomes at the national level. This study aims to examine the association of metabolic multimorbidity with health service utilization, spending, functional and mental health.Materials and Methods: This is a nationally representative cross-sectional study, utilizing the data from the China Health and Retirement Longitudinal Study in 2015, including 11,377 participants aged 45 years and older. Multivariable regression models were used to assess the association of metabolic multimorbidity with healthcare, out-of-pocket expenditure (OOPE), the activities of daily living (ADL) limitation, the instrumental activities of daily living (IADL) limitation, and depression.Results: Overall, 30.50% of total participants had metabolic multimorbidity in 2015 in China. Compared with single disease, metabolic multimorbidity were associated with the number of outpatient visits [incident rate ratio (IRR) = 1.30, 95% CI = 1.05, 1.62] and days of inpatient care (IRR = 1.52, 95% CI = 1.28, 1.81). Metabolic multimorbidity was positively associated with the OOPE on outpatient care (coefficient = 82.99, 95% CI = 17.70, 148.27) and physical functional difficulties, including ADL limitation (odds ratio = 1.36, 95% CI = 1.18, 1.57).Conclusions: Metabolic multimorbidity is associated with higher levels of health-care service use, greater expenditure for outpatient care, and more difficulties in ADL among Chinese adults. China's health-care systems need to shift from single-disease models to new financing and service delivery models to effectively manage metabolic multimorbidity.
Comparison of Transmittance and Reflectance Pulse Oximetry in Anesthetized Dogs
Authors: Nixdorff Jan , Zablotski Yury , Hartmann Katrin , Dörfelt Rene 
Published: April 2021

Objectives:The tongue is the standard site for placement of a pulse oximeter probe but is difficult to access during certain procedures such as dental and ophthalmic procedures and computerized tomography of the head. The aim of this study was to evaluate the performance of a new-generation reflectance pulse oximeter using the tail and tibia as sites for probe attachment.Materials and Methods:A total of 100 client-owned dogs that underwent anesthesia for various reasons were premedicated with butorphanol (n= 50; 0.2 mg/kg; group BUT) or butorphanol and dexmedetomidine (n= 50; 5 μg/kg; group DEX), administered intravenously. Anesthesia was induced with propofol and maintained with sevoflurane. A transmittance pulse oximeter probe was placed on the tongue and served as the reference standard. A reflectance probe was randomly placed on the tail base or the proximal tibia, and the position changed after testing. Signals from three consecutive measurements were obtained at each position. Failure was defined as “no signal,” “low signal,” or a pulse difference &gt;10/min compared with the ECG heart rate. Data were analyzed using chi-square test, Wilcoxon matched-pair signed-rank test, and Bland-Altman analysis.P&lt; 0.05 was considered significant.Results:In both groups (BUT and DEX), failure rate was higher when the tibia and tail were used as probe sites compared with the tongue. In both groups, the failure rate was higher for the tibia than for the tail. Dexmedetomidine-induced vasoconstriction increased failure rate at all probe positions.Clinical Significance:The tail base, but not the tibia, is an acceptable position for reflectance pulse oximeter probes in dogs. The tongue remains the probe site of choice, if accessible.
Age and Gender Specific Lung Cancer Incidence and Mortality in Hungary: Trends from 2011 Through 2016
Authors: Tamási Lilla , Horváth Krisztián , Kiss Zoltán , Bogos Krisztina , Ostoros Gyula , Müller Veronika , Urbán László , Bittner Nóra , Sárosi Veronika , Vastag Aladár , Polányi Zoltán , Nagy-Erdei Zsófia , Daniel Andrea , Nagy Balázs , Rokszin György , Abonyi-Tóth Zsolt , Moldvay Judit , Vokó Zoltán , Gálffy Gabriella 
Published: April 2021

Objective: No assessment was conducted describing the age and gender specific epidemiology of lung cancer (LC) prior to 2018 in Hungary, thus the objective of this study was to appraise the detailed epidemiology of lung cancer (ICD-10 C34) in Hungary based on a retrospective analysis of the National Health Insurance Fund database.Methods: This longitudinal study included patients aged ≥20 years with LC diagnosis (ICD-10 C34) between January 1, 2011 and December 31, 2016. Patients with different cancer-related codes 6 months before or 12 months after LC diagnosis or having any cancer treatment other than lung cancer protocols were excluded.Results: Lung cancer incidence and mortality increased with age, peaking in the 70–79 age group (375.0/100,000 person-years) among males, while at 60–69 age group for females (148.1/100,000 person-years). The male-to-female incidence rate ratio reached 2.46–3.01 (p &lt; 0.0001) among the 70–79 age group. We found 2–11% decrease in male incidence rate at most age groups, while a significant 1–3% increase was observed in older females (&gt;60) annually during the study period.Conclusion: This nationwide epidemiology study demonstrated that LC incidence and mortality in Hungary decreased in younger male and female population, however we found significant increase of incidence in older female population, similar to international trends. Incidence rates peaked in younger age-groups compared to Western countries, most likely due to higher smoking prevalence in these cohorts, while lower age LC incidence could be attributed to higher competing cardiovascular risk resulting in earlier mortality in smoking population.
Bloom Time Effect Depends on Muscle Type and May Determine the Results of pH and Color Instrumental Evaluation
Authors: Knecht Damian , Duziński Kamil , Jankowska-Mąkosa Anna 
Published: April 2021

The aim of this study was to determine the effect of 30 min bloom time and the type of muscle on pH and color parameters together with the possibility of estimating these measurements. The research material consisted of 270 samples from 6 muscle types: LD—Longissimus dorsi, LL—Longissimus lumborum, IL—Iliacus, SEM—Semimembranosus, CT—Cutaneous trunci, LTD—Latissimus dorsi. Measurements included pH and color of fresh pork at 0 min, and after 30 min bloom time. Bloom time influenced all analyzed parameters, although to a varying effect, depending on the muscle type. The lowest pH values were noted for dorsal-located muscles (LD, LL), then in the ham area (IL, SEM), and the highest values of the location on the side surface of the carcass (CT, LTD). The large increase in the proportion of L* and a* was observed for CT muscle (20–30%, the highest of all observed) and LTD (20–25%); for LD and LL the largest growth changes were observed for parameters b* (15–20%) and H* (20–30%). The lowest number of strong correlations was noted for LD and CT muscles, and the largest for SEM. A very good fit (R2 > 0.90) of regression equations was achieved in 7 cases. The presented results are an important contribution to the rapid and precise instrumental evaluation of pH and color.
Spatial Distribution of Private Gene Mutations in Clear Cell Renal Cell Carcinoma
Authors: Moore Ariane L. , Batavia Aashil A. , Kuipers Jack , Singer Jochen , Burcklen Elodie , Schraml Peter , Beisel Christian , Moch Holger , Beerenwinkel Niko 
Published: April 2021

Intra-tumour heterogeneity is the molecular hallmark of renal cancer, and the molecular tumour composition determines the treatment outcome of renal cancer patients. In renal cancer tumourigenesis, in general, different tumour clones evolve over time. We analysed intra-tumour heterogeneity and subclonal mutation patterns in 178 tumour samples obtained from 89 clear cell renal cell carcinoma patients. In an initial discovery phase, whole-exome and transcriptome sequencing data from paired tumour biopsies from 16 ccRCC patients were used to design a gene panel for follow-up analysis. In this second phase, 826 selected genes were targeted at deep coverage in an extended cohort of 89 patients for a detailed analysis of tumour heterogeneity. On average, we found 22 mutations per patient. Pairwise comparison of the two biopsies from the same tumour revealed that on average, 62% of the mutations in a patient were detected in one of the two samples. In addition to commonly mutated genes (VHL, PBRM1, SETD2 and BAP1), frequent subclonal mutations with low variant allele frequency (<10%) were observed in TP53 and in mucin coding genes MUC6, MUC16, and MUC3A. Of the 89 ccRCC tumours, 87 (~98%) harboured private mutations, occurring in only one of the paired tumour samples. Clonally exclusive pathway pairs were identified using the WES data set from 16 ccRCC patients. Our findings imply that shared and private mutations significantly contribute to the complexity of differential gene expression and pathway interaction and might explain the clonal evolution of different molecular renal cancer subgroups. Multi-regional sequencing is central for the identification of subclones within ccRCC.
Assessing Temporal Patterns and Species Composition of Glass Eel (Anguilla spp.) Cohorts in Sumatra and Java Using DNA Barcodes
Authors: Wibowo Arif , Hubert Nicolas , Dahruddin Hadi , Steinke Dirk , Suhaimi Rezki Antoni , Samuel  , Atminarso Dwi , Anggraeni Dian Pamularsih , Trismawanti Ike , Baumgartner Lee J. , Ning Nathan 
Published: April 2021

Anguillid eels are widely acknowledged for their ecological and socio-economic value in many countries. Yet, knowledge regarding their biodiversity, distribution and abundance remains superficial—particularly in tropical countries such as Indonesia, where demand for anguillid eels is steadily increasing along with the threat imposed by river infrastructure developments. We investigated the diversity of anguillid eels on the western Indonesian islands of Sumatra and Java using automated molecular classification and genetic species delimitation methods to explore temporal patterns of glass eel cohorts entering inland waters. A total of 278 glass eels were collected from monthly samplings along the west coast of Sumatra and the south coast of Java between March 2017 and February 2018. An automated, DNA-based glass eel identification was performed using a DNA barcode reference library consisting of 64 newly generated DNA barcodes and 117 DNA barcodes retrieved from BOLD for all nine Anguilla species known to occur in Indonesia. Species delimitation methods converged in delineating eight Molecular Operational Taxonomic Units (MOTUs), with A. nebolusa and A. bengalensis being undistinguishable by DNA barcodes. A total of four MOTUs were detected within the glass eel samples, corresponding to Anguilla bicolor, A. interioris, A. marmorata, and A. nebulosa/A. bengalensis. Monthly captures indicated that glass eel recruitment peaks in June, during the onset of the dry season, and that A. bicolor is the most prevalent species. Comparing indices of mitochondrial genetic diversity between yellow/silver eels, originating from several sites across the species range distribution, and glass eels, collected in West Sumatra and Java, indicated a marked difference. Glass eels displayed a much lower diversity than yellow/silver eels. Implications for the management of glass eel fisheries and species conservation are discussed.
A Comparison of Technology Teacher′s Personal Background, Teaching Environment, Behavior and Psychological Characteristics of Korean and U.S.: Using TALIS 2018 data
Authors:   Korean Technology Education Association, Kim Young-Heung 
Published: April 2021

The purpose of this study was to compare the human background and environment and behavioral and psychological characteristics of Korean and American technical teachers. Subject to analysis are 214 South Korean and 675 U.S. technology teachers who participated in TALIS 2018 survey. Major research results show that Korean and American technology teachers tend to be similar to middle school teachers. Second, the annual working hours of Korean technical teachers are less than those of the United States, but there is a lot of administrative time, and there is a relatively little time to allocate to classes and professional development. Third, Korean technical teachers are relatively lacking in professional efforts such as obtaining degrees to develop expertise, and activities to develop curriculum contents and teaching methods are active, but efforts related to innovation such as multiculturalism and ICT utilization are lacking. Fourth, Korean technical teachers lack high-dimensional teaching
activities and are having difficulty guiding students. Fifth, Korean technical teachers have less class cooperation activities among fellow teachers. Sixth, Korean technical teachers choose teaching as motivation for personal usefulness, such as job stability and time-lapse. Seventh, Korean technical teachers have a relatively lower sense of effectiveness than the United States, especially having difficulty in class management, and low job satisfaction, which makes them less satisfied with their working environment.
ANALISIS PREFERENSI KONSUMEN DALAM MEMBELI DAGING BROILER DI PASAR TRADISIONAL KOTA MANADO (STUDI KASUS “PASAR PINASUNGKULAN KAROMBASAN”)
Authors: Simarmata Lasmaria , Osak R E.M.F , Endoh E K.M , Oroh F N.S 
Published: May 2021

ABSTRACT ANALYSIS OF CONSUMER BUYING PREFERENCES IN BROILER MEAT AT TRADITIONAL MARKET IN MANADO CITY (CASE STUDY OF THE "PINASUNGKULAN KAROMBASAN MARKET"). The goals of this study was to analyze consumer preference and attitude on attribute variation of broilers at the traditional market in Manado. Study was conducted at the Pinasungkulan market, Karombasan Manado on February 2019. Survey method was used by deep interview supported by questionnaire. Samples were defined by accidental sampling method involving fifty respondents. The primer and secondary data were used in this study and analyzed by Chi-Square and Multi attribute Fishbein analysis. The Chi-Square analysis indicated that all attributes studied were significantly different. This was meaning that there were differences of consumers preference on broilers. On the basis of Multi attribute Fishbein analysis indicated that all broiler attribute were considered by consumers in making decision of buying broilers. Broilers were became consumer preference significantly at the Pinasungkulan Market, meaning that there was difference of consumer preference on broiler attributes. Sequences of broiler attribute from the highest consideration to the lowest consideration were meat color, skin color, meat smell, skin tenderness and meat weight. Consumer attitude on broiler meat attributed in decision of buying meat of broilers was broiler meat color. Keywords: Broiler, consumer preference