Data-dependent visualization of biological networks in the web-browser with NDExEdit
Authors: Auer Florian , Mayer Simone , Kramer Frank 
Published: November 2021

AbstractNetworks are a common methodology used to capture increasingly complex associations between biological entities. They serve as a resource of biological knowledge for bioinformatics analyses, and also comprise the subsequent results. However, the interpretation of biological networks is challenging and requires suitable visualizations dependent on the contained information. The most prominent software in the field for the visualization of biological networks is Cytoscape, a desktop modeling environment also including many features for analysis.A further challenge when working with networks is their distribution. Within a typical collaborative workflow, even slight changes of the network data force one to repeat the visualization step as well. Also, just minor adjustments to the visual representation not only need the networks to be transferred back and forth. Collaboration on the same resources requires specific infrastructure to avoid redundancies, or worse, the corruption of the data. A well-established solution is provided by the NDEx platform where users can upload a network, share it with selected colleagues or make it publicly available.NDExEdit is a web-based application where simple changes can be made to biological networks within the browser, and which does not require installation. With our tool, plain networks can be enhanced easily for further usage in presentations and publications. Since the network data is only stored locally within the web browser, users can edit their private networks without concerns of unintentional publication. The web tool is designed to conform to the Cytoscape Exchange (CX) format as a data model, which is used for the data transmission by both tools, Cytoscape and NDEx. Therefore the modified network can be exported as a compatible CX file, additionally to standard image formats like PNG and JPEG.Author summaryRelations in biological research are often visualized as networks. For instance, if two proteins interact with each other during a certain process, the corresponding network would show two nodes connected by one edge. But the fact that the interaction between the two exists, may not be enough. With established software solutions like Cytoscape we can add all the information we have about our nodes and their interaction to our data foundation. Furthermore, we can change the visual appearance of our nodes and their interaction based on this information.For example, if our network contains 20 nodes, that all interact with each other, but the strength of these interactions each range between 0 and 1, we can illustrate that by making the edges wider for strong interactions and slimmer for weak interactions. Thus, our visualization is enriched with valuable information. As of now these data-dependent modifications can only be made with a desktop client.We introduce NDExEdit, a web-based solution for visualization changes to networks that conform to the CX data format. It allows us to import networks directly from the NDEx platform and apply changes to the visualization – including all types of mappings, one of which was briefly described above.