bluuebunny/crossref_metadata_2025_split huggingface dataset:
Synthesis and Anticancer Activity of Novel Hydrazone Linkage-Based Aryl Sulfonate Derivatives as Apoptosis Inducers
Authors: şenkardeş sevil , Han M.İhsan , Gürboğa Merve , Özakpınar Özlem Bingöl , Küçükgüzel Ş. Güniz 
Published: November 2021

Abstract
In the present study, the various 28 hybrid molecules containing hydrazone and sulfonate moiety were synthesized and characterized by FTIR, 1H-NMR, 13C-NMR spectroscopy and LC-MS spectrometry, besides elemental analysis. The compounds were evaluated for their antiproliferative effects against six cancer cell lines, namely A549 (non-small cell lung cancer), MCF-7 (breast cancer), HT-29 (colorectal adenocarcinoma cancer), PC-3 (androgen-independent prostate adenocarcinoma), Hep3B (hepatocellular carcinoma cancer), and HeLa (epitheloid cervix carcinoma cancer). Among all the target compounds, compounds 4g and 4h exhibited more promising effects on MCF-7 cell lines (IC50=17.8 mM and 21.2 mM, respectively) with high selectivity. Further mechanistic studies proposed that compounds 4g and 4h induced apoptosis is mediated through the intrinsic apoptotic pathway with changes in mitochondrial membrane potential by finally activating caspase-9 and caspase-3. The results have been encouraging enough to merit further investigation.
Survey On Disclosing Information To Children With Genetic Conditions And Their Siblings In Japan
Authors: Kaneko Mikiko , Oba Daijyu , Ohashi Hirofumi 
Published: November 2021

Abstract
Many parents face the dilemma of when, how, and what to disclose to their children regarding their genetic conditions. The purpose of this study was to learn about the experiences of parents regarding disclosing information to their children with genetic conditions.Methods: A questionnaire was sent to 378 parents of children and adolescents with the following genetic conditions: 22q11.2 deletion syndrome, Beckwith-Wiedemann syndrome, Noonan syndrome, Russell-Silver syndrome, Kabuki syndrome, Williams syndrome, Prader-Willi syndrome, and Sotos syndrome. Findings were analyzed using descriptive statistics for multiple-choice questions and thematic analysis for open-ended questions.Results: Of the parents surveyed, 158 (41.8 %) responded to the questionnaires. The average age of children with genetic conditions was 12 years. Sixty-seven parents had disclosed relevant information to their children, whereas 91 had not. Sixty-eight out of 91 respondents were planning to disclose information in the future. Many respondents who had disclosed information did not regret this. They felt good talking about genetic conditions, and had talked about genetic conditions with the affected children following disclosure.Conclusion: This study contributed to our understanding of the attitudes of parents towards disclosing information to children with genetic conditions.
Pancreatic Safety of DPP-4 Inhibitors in Type 2 Diabetes Millitus—Protocol for a Systematic Review and Network Meta-Analysis of Randomized Controlled Trials
Authors: Yang Fan , Zhao Quanlin , Dong Youzi , Li Baohua , Ning Bobiao 
Published: November 2021

Abstract
Background: Dipeptidyl-peptidase IV inhibitor (DPP-4i) is a common hypoglycemic medication in treating type 2 diabetes millitus (T2DM). It has become widely utilized in clinical practice due to its ability to effectively manage blood glucose while posing a low risk of hypoglycemia and weight gain. However, there is no consensus on DPP-4i's pancreatic safety due to a paucity of clinical evidence. The safe event appears to be easily overlooked. This review aims to evaluate the pancreatic safety of DPP-4i in patients with type 2 diabetes mellitus using the standard pairwise and network meta-analysis (NMA) methods.Methods: MEDLINE, Embase, Pubmed, Web of Science, and the Cochrane Central Register of Controlled Trials will be used to search for published literature and clinical trial registries (such as www.ClinicalTrials.gov) will be used to look for unpublished trials. English-language randomized controlled trials (RCTs) will be included. Two independent reviewers will screen literature for eligibility, extract available data, and assess the risk of bias. All divergences will be resolved after rechecking the source papers and further discussion among the reviewers with a complete consensus before inclusion. Pancreatic safety events involving acute pancreatitis, chronic pancreatitis, and pancreatic cancer will be the primary outcome. The secondary result will be the change in pancreatic enzyme from the baseline. A random-effects model will be used for pairwise meta-analysis with a 95% confidence interval, and Bayesian network meta-analysis will be conducted between the various interventions.Discussion: This systematic review and network meta-analysis will evaluate the pancreatic safety of DPP-4i in patients with type 2 diabetes millitus. The findings of this study may supplement the evidence-based information on DPP-4i, improve existing understanding of this issue, and assist patients and clinicians in making better treatment decisions by raising their awareness of the problem. Systematic review registration: submitted in PROSPERO, 31 August 2021
Productivity Impact of Climate Change on Primary Export Commodity in Africa
Authors: Gebrehiwot Berihu Assefa , Weldesilassie Alebel B. 
Published: November 2021

Abstract
Using a large household survey dataset and climate data, we estimated and analyzed the productivity impact of climate change on two strategic export commodities, cotton, and sugarcane, in Ethiopia. The productivity impact revealed that it is likely that climate change will significantly reduce the productivity of sugarcane and cotton. In addition, net revenue from sugarcane and cotton will also be reduced due to an increase in annual and seasonal temperature as well as changes in mean annual and seasonal rainfall. We have presented results for various climate scenarios and seasons. We also found that non–climate drivers of vulnerability such as gender, education, access to extension services and credit, technology use, access to land and non – farm income, and natural assets will exacerbate the negative impacts of climate change.
Identification of Key Genes and Competitive Endogenous RNA Network Construction in Osteonecrosis of the Femoral Head by Integrated Bioinformatics Analysis
Authors: Dinglong Yang , Shuai Chen , Yujing Chen , Beiyang Wang , Guohao Zhang , Zhiqiang Zhang 
Published: November 2021

Abstract
Background: Despite cumulative evidence shows osteonecrosis of the femoral head (ONFH) could result in the progressive collapse of the femoral head. The pathogenesis of ONFH remains unclear. Early ONFH is difficult to diagnose due to the lack of effective biomarkers. Method: In Gene Expression Omnibus (GEO) database, we searched the Microarray datasets for serum (GSE123568) in ONFH and normal controls to identify differentially expressed genes (DEGs) by R software. The enrichment analyses were performed to enrich pathways of DEGs. Protein–protein interaction (PPI), miRNA-mRNA co-expression, ceRNA networks were constructed using Cytoscape to identity top15 hub genes, target miRNAs of hub genes and potential regulatory pathways. Furthermore, hub genes validated in GSE74089 with high diagnostic value for ONFH were selected as key genes. The Human Protein Atlas (HPA) and Bgee Database were used to find out the subcellular and tissue distribution of key genes.Results: A total of 568 DEGs were identified between 30 ONFH samples and 10 normal controls. Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO) enrichment analysis showed that DEGs are mostly enriched in innate immune responses, thrombosis and signal transduction. Fifteen hub genes were identified by PPI network using Cytoscape. The 15 hub genes were almost all positively correlated with each other. The expression of PLEK (P＜0.001), TLR2 (P＜0.05), and TREM1 (P＜0.001) were validated in dataset GSE74089 and they had high diagnostic value (AUC＞0.8) for ONFH. MALAT1-miR-146b-5p-TLR2, MALAT1-miR-664b-3p-PLEK, NORAD-miR-106b-5p-TLR2, and MSMO1-miR-106b-5p-TLR2 might be potential RNA regulatory pathways in the disease progression of ONFH. PLEK mainly expressed in nucleus, TREM1 in dictyosome, and TLR2 in nucleoplasm and mitochondria.Conclusions: In this study, we found that PLEK, TLR2, and TREM1 might be potential biomarkers in diagnostic and play a vital role in the progression of ONFH.
A nomogram for predicting overall survival in patients with Merkel cell carcinoma: A population-based analysis
Authors: Wang Miao , Qiu Ye , Tang Fang , Liu Yi-Xin , Li Yi , Wang Xu-Long , Li Shi-Yi , He Li , Zhang Bo-Yu , Liang Wen-Jing , Wei Yongchang 
Published: November 2021

Abstract
Background: Merkel cell carcinoma (MCC) is a rare neuroendocrine skin cancer with increasing incidence and poor prognosis. We sought to develop and validate a nomogram to estimate overall survival (OS) of MCC patients. Methods: 1863 MCC patients between 2010-2015 from the Surveillance, Epidemiology and End Results (SEER) database were randomly divided into the training and validation cohort. Independent prognostic factors determined by Cox regression analysis in the training cohort were used to establish a nomogram. We evaluated prognostic performance using the concordance index (C-index), area under receiver operating characteristic curve (AUC) and calibration curves. Decision curve analysis (DCA), net reclassification index (NRI) and integrated discrimination improvement (IDI) were used to compared the the nomogram’s clinical utility with that of the staging system.Results: eight independent prognostic factors were incorporated in the nomogram. The C-index of the nomogram was 0.744, which was superior to the C-index of AJCC TNM Stage (0.659). The AUC was greater than 0.75 and the calibration plots of this model exhibited good performance. Additionally, the positive NRI and IDI of nomogram versus the staging system illustrated that the nomogram had better predictive accuracy than the staging system (P<0.001) and the DCA showed great clinical usefulness of the nomogram. MCC patients were perfectly classified into three risk groups by the nomogram, showing better discrimination than the staging system.Conclusions: We developed and validated a nomogram to assist clinicians in evaluating prognosis of MCC patients.
Effect of Footwear on Running Impact Loading in the Preschool Years
Authors: Plesek Jan , Hamill Joseph , Blaschova Denisa , Silvernail Julia Freedman , Jandacka Daniel 
Published: November 2021

Abstract
Background
Previous research indicated that running barefoot or in minimalist shoes led to lower impact loading in an adolescent and adult population. Running as fundamental locomotor skill significantly develops during early childhood (preschool age). However, no study has focused on effect of footwear condition on lower limb impact loading during running in this age. Therefore, the purpose of this study was to assess effect of footwear conditions (barefoot, minimalist and standard running shoes) on running impact loading in the preschool years.
Methods
Fourty-eight habitually shod preschool children were divided into 4 age groups. Children performed simple running game in 3 different footwear conditions (random counter-balanced order), 3-dimensional biomechanical analysis were carried out during overground running. The key dependent variables included vertical ground reaction force (VGRF) and vertical instantaneous loading rate (VILR). Statistical parametric mapping was performed to reveal possible differences in VGRF and one-way repeated measures ANOVA in VILR.
Results
Three-year-old children displayed significantly lower impact peak of VGRF in barefoot condition compared to minimalist (3-7% stance, P = 0.012) and standard running shoes (7-11% stance, P = 0.009). Furthermore, in 3-year-old in minimalist shoes had higher loading than in standard running shoes (0-4% stance, P = 0.007). There were also differences in VILR, where 3-year-old had lower loading in barefoot than in minimalist (P = 0.010, d = 1.19) or standard running shoes (P = 0.045, d = 0.98). No differences were found in older children.
Conclusion
Running in minimalist shoes did not imitate barefoot running and did not lower impact forces compared to standard running shoes in 3-year-old children. On the contrary, increased loading was observed in minimalist shoes in early running developmental stages. Professionals who work with children should consider effect of minimalist shoes on impact loading (running on hard surfaces).
The Use of Wavelet Transform To Evaluate The Sensitivity of Acoustic Emission Attributes To Variation of Cutting Parameters in Milling Aluminium Alloys
Authors: Asadi Reza , Anahid Mohamad Javad , Heydarnia Hoda , Mehmanparast Hedayeh , Niknam Seyed Ali 
Published: November 2021

Abstract
Appropriate acquisition and assessment of the dominant acoustic emission (AE) signal attributes generated under various experimental cutting conditions may provide significant knowledge. Consequently, it enhances the efficiency in manufacturing process monitoring and control. However, according to the literature, a lack of information was noticed on the behavior of AE signal attributes under various cutting conditions. Considering that milling is among the most widely used machining operations, the aim of this investigation is to acquire adequate knowledge about interactions between cutting parameters and their direct and indirect effects on the obtained AE signals attributes from the milling process. In the course of this work, the effects of cutting conditions on the attributes calculated from wavelet transform (WT) of AE signals will be presented. WT signal processing was conducted with five models of mother wavelets, and appropriate decomposition numbers were deployed. The approximated signal attributes obtained from each decomposition were assessed. According to signal processing and statistical calculations, cutting speed, feed rate, and coating significantly impacted the variation of AE signal attributes. Also, the most sensitive AE signal attributes and decompositions were rms, std, entropy and energy, and 2nd and 6th decompositions, respectively. The outcome of this work can be integrated into advanced artificial intelligence (AI) approaches to implement real-time monitoring of manufacturing processes.
Clinical Significance and Regulatory Network of Mast Cells in Early-Stage Lung Adenocarcinoma
Authors: Wang Zhen , Luo Bin , Duan Pengfei , Yang Han , Lin Peng 
Published: November 2021

Abstract
Background The role and upstream regulation of mast cells (a crucial constituent of the tumor immune microenvironment) in early-stage lung adenocarcinoma (LUAD) remains unclear. Methods Using CIBERSORT, we analyzed the expression profile downloaded from the NCBI Gene Expression Omnibus (GEO) database. Next, using weighted gene coexpression network analysis (WGCNA) and enrichment analysis, we identified upstream regulators of mast cells. Finally, we verified their relevance using data from The Cancer Genome Atlas (TCGA) and Genotype-Tissue Expression (GTEx) databases. Results Among patients with early-stage LUAD, those with high mast cell infiltration levels had a better prognosis, and mast cell infiltration levels were closely associated with the mutation status of TP53, STK11 and EGFR. CAMK2D, AURKA, AURKB, PLK1 and EZH2 showed strong correlations with mast cell infiltration and a key growth factor of mast cells (KITLG), and these 5 regulators were closely correlated with the survival of patients with early-stage lung adenocarcinoma. Conclusion Mast cells play a protective role in patients with early-stage LUAD. We speculate that CAMK2D, AURKA, AURKB, PLK1 and EZH2 promote or inhibit the infiltration of mast cells in early-stage lung adenocarcinoma tissues by regulating KITLG, likely serving as novel targets in immunotherapy for LUAD.
The Role of Pre-operative MRI For Prediction of High-Grade Intracranial Meningioma: A Retrospective Study
Authors: Lekhavat Vitit , Radeesri Kan 
Published: November 2021

Abstract
Introduction: High histological grade (WHO grade II and III) intracranial meningiomas have been linked to greater risk for tumor recurrence and worse clinical outcomes compared to low-grade (WHO grade I) tumors. Preoperative magnetic resonance imaging (MRI) plays a crucial role tumor evaluation prior to decisions regarding management and allows for a better understanding of the tumor grading, which could potentially alter clinical outcomes. The present study sought to determine whether preoperative MRI features of intracranial meningiomas can serve as predictors of high-grade tumors.Methods: This study retrospectively reviewed 327 confirmed cases of intracranial meningiomas, among whom 210 (64.2%) had available preoperative MRI studies. Thereafter, data were analyzed using univariate and multivariate analyses.Results: Accordingly, multivariate analysis found that peritumoral brain edema and the presence of necrosis or hemorrhage were predictors of high-grade tumors, whereas hyperostosis was a predictor of low-grade tumors.Conclusions: Our study suggested that preoperative MRI features could potentially assist in decision-making regarding the appropriate management and surgical approach in order to achieve the desired clinical outcomes.
Novel Lung Targeting Cell Penetrating Peptides as Vectors for Delivery of Therapeutics
Authors: Zahid Maliha , McCandless Kayla , Mishra Sanjay , Stiltner Jeffrey , Feldman Kyle , Yagi Hisato , Yurko Raymond , Islam Kazi , Brown Jonathan , Frizzell Raymond 
Published: November 2021

Abstract
Cell penetrating peptides are unique, 5-30 amino acid long peptides that are able to breach cell membrane barriers and carry cargoes intracellularly in a functional form. Our prior work identified a synthetic, non-naturally occurring 12-amino acid long peptide that we termed cardiac targeting peptide (CTP: APWHLSSQYSRT) due to its ability to transduce cardiomyocytes in vivo. Studies looking into its mechanism of transduction identified two lung targeting peptides (LTPs), S7A (APWHLSAQYSRT) and R11A (APWHLSSQYSAT). These peptides robustly transduced human bronchial epithelial cell lines in vitro and mouse lung tissue in vivo. This uptake occurred independently of clathrin mediated endocytosis. Biodistribution studies of R11A showed peak uptake at 15 minutes with uptake in liver but not kidneys, indicating primarily a hepatobiliary mode of excretion. Cyclic version of both peptides was ~100-fold more efficient in permeating cells than their linear counterparts. As proof of principle, we conjugated anti-spike and anti-envelope SARS-CoV-2 siRNAs to cyclized R11A and demonstrate anti-viral efficacy in vitro. Our work presented here identifies two novel lung-specific cell penetrating peptides that could potentially deliver myriad therapeutic cargoes to lung tissue.
Clinicopathologic Characteristics, Therapeutic Modalities and Survival Outcomes of Plasmablastic Lymphoma: A Real-World Study
Authors: Zheng Yan-Hua , Wang Hai-Tao , Wan Zhuo , Tian Biao , Shen Hong-Yuan , Liu Li , Feng Juan 
Published: November 2021

Abstract
Introduction: Plasmablastic lymphoma(PBL)，an extremely rare subtype of B-cell non-Hodgkin lymphoma(NHL), is characterized by aggressiveness, rapid progression and bleak prognosis. Neither standardized regimen nor consensus for PBL treatment has been established.Methods: We retrospectively analyzed the clinicopathologic characteristics, therapeutic modalities and survival outcomes of 418 patients registered in the Surveillance, Epidemiology, and End Results (SEER) database from 2008 to 2016 and 21 (19 treated) patients in our institution. Kaplan-Meier survival curves and log-rank test for overall survival (OS) and cancer-specific survival (CSS) were performed to compare each variable. Variables with statistical significance in the univariate Cox regression were incorporated into the multivariate Cox model to determine the independent prognostic factors.Results: In patient cohort from the SEER, PBL has a striking male predilection. The median OS for all PBL patients was 17 months. The 1-year,3-year and 5-year OS rate were 54.4%, 40.4% and 37.2% respectively. Patients who suffered from previous malignancy had a significant survival disadvantage compared to those without previous cancer. Patients with higher Ann Arbor stage at diagnosis were at higher risk of death than those with lower stage. Chemotherapy alone or chemotherapy combined with radiotherapy could significantly reduce the risk of death and extend the patients’ survival, yielding HR of 0.209(95%CI 0.152-0.288) and 0.187(95%CI 0.089-0.394), respectively. Radiation alone seemed useless. All patients from our institution were HIV-negative. The main therapeutic regimens were CHOP or CHOPE, DA-EPOCH, DHAP and ESHAP. Complete response (CR) was achieved in only 3 patients, while partial response (PR) in 10 patients. The median OS was 7 months. Fourteen patients later died of the disease progression.Conclusions: Previous malignancy history, Ann Arbor stage and therapeutic modality were independent prognostic factors. Bortezomib combined with DA-EPOCH may serve as an effective regimen for PBL. The optimal therapeutic modality necessitates further exploration.
Influencing Factors of Humanistic Care Ability in Mental Health Workers: An Online Cross-Sectional Study
Authors: Liu Xiaolin , Zhu Hongjin 
Published: November 2021

Abstract
Background Mental health workers may face more obstacles in humanistic care during the COVID-19 pandemic, we aimed to explore the humanistic care ability of mental health workers and its potential influencing factors.Method(s) An online cross-sectional survey was conducted among 262 mental health workers working in Chongqing, China from December 1 to 31, 2020. Self-administered questionnaires were used to collect data from the participants. Results Mental health workers’ humanistic care ability score was 186.47 ± 21.34, and most of the them (79.8%) were at a low level. Psychological capital and personality traits were significant influencing factors of humanistic care ability and its sub-dimensions.Conclusion(s) Research has found that the humanistic care ability of mental health workers is at a low level, and psychological capital and personality traits are significant factors influencing the humanistic care ability and its sub-dimensions. Interventions to improve psychological capital of mental health workers or to promote the change of personality traits that they want are recommended, thereby promoting humanistic practice.