bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification and Validation of lncRNA-Related Prognostic Signatures in Cholangiocarcinoma
Authors: Zhang JiaNing , Li Fengwei , Huang Yihua , Xue Hui , Tao Qifei , Zhuang Guokun , Ding Dongyang , Xuan Jianbing , Wang Kui , Zhou Weiping 
Published: July 2021

Abstract
Background and Aims: Cholangiocarcinoma (CCA), the second most common hepatobiliary cancer, is associated with poor prognosis. Therefore, there is a need to elucidate on the pathogenic mechanisms of CCA. In this study, we aimed at identifying lncRNA-related prognostic signatures for CCA through bioinformatics analysis and further validated their functions in CCA tumorigenesis and progression.Methods: The RNA-seq data of CCA were downloaded from public databases. Differentially expressed lncRNAs (DElncRNAs) were screened using R packages. Then, candidate OS- and DFS-related DElncRNAs were selected through Kaplan–Meier survival analysis. Furthermore, LASSO regression analyses were performed to establish two prognostic signatures, termed the OS and DFS signatures, respectively. Multivariate COX models and nomograms for overall survival (OS) and disease-free survival (DFS) were established based on OS/DFS signature and clinical data. Hub lncRNAs were identified and enrichment analyses performed to explore their potential functions. Finally, in vitro and in vivo models were used to validate the effects of the hub lncRNAs in CCA tumorigenesis and progression.Results: A total of 925 DElncRNAs were selected, from which six candidate OS-related lncRNAs and 15 candidate DFS-related lncRNAs were identified. The OS and DFS signatures were then established using four lncRNAs, respectively. We found that the OS signature and vascular invasion were significant independent risk factors for OS outcomes of CCA, while the DFS signature, vascular invasion and CA19-9 were significant independent risk factors for DFS outcomes of CCA. MiR4435-2HG and GAPLINC were selected as hub lncRNAs because they were included in both OS and DFS signatures. GO and KEGG enrichment analyses revealed that the two hub lncRNAs were involved in CCA tumorigenesis and progression. Finally, we constructed in vitro and in vivo models and revealed that the lncRNAs, MiR4435-2HG and GAPLINC can prompt CCA proliferation and migration in vitro and in vivo.Conclusions: The established OS and DFS signatures, which were based on DElncRNAs, are independent risk factors for OS and DFS of CCA patients, respectively. MIR4435-2HG and GAPLINC were identified as hub lncRNAs. In vitro and in vivo models revealed that MiR4435-2HG and GAPLINC can prompt CCA progression, which might be novel prognostic biomarkers and therapeutic targets for CCA.
De-escalation of Antiplatelet Therapy Retains P2Y12 Inhibitor After Percutaneous Coronary Intervention Among East Asians and Non-East Asians: A Meta-Analysis of Randomized Controlled Trials
Authors: Wang Ge , Wang Xiao , Zhao Guanqi , Huang Xin , Guo Ruifeng , Nie Shaoping 
Published: July 2021

Abstract
Background: The impact of de-escalation of antiplatelet therapy retains P2Y12 inhibitor on major bleeding and ischemic outcomes after percutaneous coronary intervention (PCI) among East Asians and non-East Asians was unclear.Methods: We systematically searched PubMed, Embase and the Cochrane Library for randomized controlled trials through September 2020. Eight trials were included, which studied de-escalation of DAPT (D-DAPT, switching to P2Y12 inhibitor monotherapy or decreasing the intensity of P2Y12 inhibitor after 1 to 3 months) versus 12 months standard DAPT (S-DAPT). The primary outcomes data were conducted with random effects models.Results: Among 8 included trials with 37,775 patients, 62.6% of patients presented with acute coronary syndrome. The median follow-up duration ranged from 12 to 24 months. Compared with S-DAPT, D-DAPT was associated with a lower risk of major bleeding (RR=0.64, 95%CI: 0.47-0.88, p=0.006), but this was only observed among East-Asians (RR=0.55, 95%CI: 0.38-0.81, p=0.002). Among non-East Asians, the rate of major bleeding was similar between two groups (RR=0.73, 95% CI: 0.46-1.14, p=0.17). There was no significant difference of MACE between D-DAPT and S-DAPT treatment among both East Asians (RR=0.84, 95%CI: 0.66-1.08, p=0.18) and non-East Asians (RR=0.89, 95%CI: 0.79-1.00, p=0.05).Conclusions: De-escalation strategy retains P2Y12 inhibitor after PCI was associated with reduced risk of bleeding events, which was only demonstrated in East Asians patients, but not in non-East Asians patients.
Landfill Site Selection via Geographical Information System and Fuzzy Logic in Uşak Province (Turkey)
Authors: Deniz Mehmet 
Published: July 2021

Abstract
The importance of landfill site selection has grown rapidly in recent years. New and various analytical techniques have also been developed due to recently conducted research. The techniques that have been developed for landfill site selection have been shaped according to the possibilities of the current time. In more recent years, along with breakthroughs in information systems technology, computer-assisted work designs and analytical techniques have come to the forefront. The intention of this study is to identify the best locations to serve as a landfill site across the provincial territory of Uşak, Turkey, while also making use of these modern technologies. This study utilizes the fuzzy logic methodology which has become common in recent years. The criteria set out in this study was digitalized through the design of base maps. ArcGIS 10.2 software was used for the formation of fuzzy membership values and fuzzy overlay procedures. The criteria that are common in the literature for fuzzy logic includes roads, rivers, airports, settlements, faults and landslides, slope, geology, soil, land use capability, and land use. As a result of this study, suitable and unsuitable locations for the purpose of landfilling were identified and mapped. These analyses show that the most optimal locations to serve as a landfill site are mostly located in the southwestern part of the Uşak province
Supramolecular nanomedicine for enhanced chemotherapy against drug-resistant colorectal cancer
Authors: Yan Xiangjie , Ma Feihe , Chen Qixian , Gou Xiangbo , Li Xiaohui , Zhang Liuwei , Gao Hui 
Published: July 2021

Abstract
The presence of Fusobacterium nucleatum (F. nucleatum) in the community of colorectal cancer (CRC) accounts for its chemotherapy resistance, which underlies the clinical failure of chemotherapeutics in the treatment of CRC. To resolve this drawback, we manufactured a novel multifaceted supramolecular nanomedicine capable of eliminating F. nucleatum, minimizing the non-specific toxicity to normal tissues, and maximizing anti-tumor efficacy. The supramolecular nanomedicine was constructed by conjugating lauric acid (LA) and platinum (IV) oxaliplatin prodrug (OxPt-COOH) to hyperbranched poly(glycidol) (PG), followed by addition of cucurbit[7]uril (CB[7]) to elicit supramolecular assembly. In principle, LA is schemed to eliminate the F. nucleatum in CRC, thereby enhancing chemotherapeutic efficacy of OxPt. CB[7] facilitates host − guest complexation with OxPt, allowing its activation specifically in the spermine-overexpressed CRC tumors and thus reducing the potential toxicity to normal tissues. Moreover, CB[7] conduces to supramolecular assembly between the self-assembly units of PG-Pt-LA nanoparticles, representing a novel size-transformable supramolecular nanomedicine (PG-Pt-LA/CB[7]), whose hydrodynamic diameter is approximately 200 nm in blood circulation and decreases to approximately 10 nm upon arrival at tumors due to its responsiveness to spermine, which can address the dilemma of long circulation and deep penetration existed in nanomedicine. In vivo investigations reveal that our proposed supramolecular nanomedicine possesses prolonged blood circulation, enhanced permeation and retention effect in tumors. In F. nucleatum co-localized HT29 mouse model, PG-Pt-LA/CB[7] is validated to be a targeted, safe and effective nanomedicine that substantially promoted the chemotherapeutic efficacy in the treatment of drug-resistant CRC through inhibiting autophagy induced by F. nucleatum. These findings demonstrate our multifaceted supramolecular nanomedicine can effectively overcome drug resistance of chemotherapy caused by bacteria, providing a valid strategy for clinic treatment of intractable CRC.
Assessment of Intracardiac and Extracardiac Anomalies in Patients with Various Types of Pulmonary Atresia by Low-dose Dual-source Computed Tomography
Authors: Qian Wen-lei , Zhou Xin-zhu , Shi Ke , Jiang Li , Liu Xi , Shen Li-ting , Yang Zhi-gang 
Published: July 2021

Abstract
Background: pulmonary atresia (PA) is a group of heterogeneous complex congenital heart disease which need more than one study modality to get correct diagnoses. This study aims to investigate the diagnostic power of low-dose dual-source computed tomography (DSCT) for all intracardiac and extracardiac anomalies in patients with PA compared with transthoracic echocardiography (TTE).Materials and methods: This retrospective study enrolled 73 patients and divided them into three groups according to their main diagnosis. All associated malformation and clinical information, including treatments, were recorded and compared among the three groups. The diagnostic power of DSCT and TTE on all associated anomalies were compared. The surgical index (McGoon ratio, pulmonary arterials index (PAI), and total neopulmonary arterial index) and radiation dose were calculated on the basis of DSCT.Results: Of the patients, 29, 29, and 15 were divide into the groups of PA with ventricular septal defect (VSD), PA with VSD and major aortopulmonary collateral arteries, and PA with other major malformations, respectively. Consequently, 178, 144, and 12 intracardiac, extracardiac, and other major anomalies were diagnosed, respectively. Moreover, DSCT showed a better diagnostic performance in extracardiac anomalies (137 vs 100, p = 0.000), whereas TTE could diagnose intracardiac anomalies better (158 vs 134, p = 0.001). The McGoon ratio, PAI, and treatment methods were significantly different among the three groups (p = 0.027, p = 0.035, and p = 0.041, respectively).Conclusion: More than one imaging modality should be used to make a correct diagnosis when clinically suspecting PA. DSCT is superior to TTE in diagnosing extracardiac anomalies and could be used to roughly calculate surgical indices to optimize treatment strategy.
The Proteome, Not the Transcriptome, Predicts that Oocyte Superovulation Affects Embryonic Phenotypes in Mice
Authors: Taher Leila , Israel Steffen , Drexler Hannes , Makalowski Wojciech , Suzuki Yutaka , Fuellen Georg , Boiani Michele 
Published: July 2021

Abstract
Superovulation is the epitome for generating oocytes for molecular embryology in mice, and it is used to model medically assisted reproduction in humans. Yet whether a superovulated oocyte is normal, is an open question. This study establishes for the first time that superovulation is associated with proteome changes that bear on phenotypic traits in mice, whereas the transcriptome is far less predictive. The proteins that were differentially expressed in superovulated mouse oocytes and their derived embryos relative to their naturally ovulated counterparts were enriched in ontology terms describing abnormal mammalian phenotypes: a thinner zona pellucida, a smaller oocyte diameter, increased cleavage arrest, and defective blastocyst formation, which we could all verify functionally. Moreover, our findings indicate that embryos with such abnormalities are negatively selected during preimplantation, and ascribe these abnormalities to incomplete ovarian maturation during the time of the conventional superovulation, since they could be corrected upon postponement of the ovulatory stimulus by 24 h. Our data place constraints on the common view that superovulated oocytes are suited to draw conclusions of general validity about developmental processes, and underscore the importance of including the proteins in a modern molecular definition of oocyte quality.
Establishment of an artificial neural network model using immune-infiltration related factors for endometrial receptivity assessment
Authors: Li Bohan , Duan Hua , Wang Sha , Wu Jiajing , Li Yazhu 
Published: July 2021

Abstract

Background
A comprehensive clinical strategy for infertility involves treatment and, more importantly, post-treatment evaluation. Endometrial receptivity, as a component of assessment, does not have a validated tool. This study was anchored on immune factors, which act as critical factors affecting embryonic implantation, was aimed at establishing novel approaches for assessing endometrial receptivity to guide clinical practice.
Methods
Immune-infiltration levels in the GSE58144 dataset (n = 115) from GEO were analyzed by digital deconvolution and validated by immunofluorescence (n = 30). Modules that were most associated with M1/M2 macrophages and their hub genes were then selected by weighted gene co-expression network as well as univariate analyses and validated by GSE5099 macrophage dataset and qPCR analysis (n = 16). Finally, the artificial neural network model was established from hub genes and its predictive efficacy was validated in the GSE165004 dataset (n = 72).
Results
Dysregulation of the ratio of M1 to M2 macrophages is an important factor contributing to decreased endometrial receptivity. Selected hub genes, RPS9, DUT, and KIAA0430, were significantly altered in patients with endometrial receptivity decreased and found relating to M1/M2 through ribosomal and proteasomal pathways, and were significantly altered in patients. The model established by the selected hub genes exhibited an excellent predictive value in both datasets with an accuracy of 98.3% and an AUC of 0.975 (95% CI 0.945-1).
Conclusions
M1/M2 polarization can influence endometrial receptivity by the three genes regulation, while the established ANN model can effectively assess endometrial receptivity to inform patients' pregnancy and individualized clinical management strategies.
Does Socioeconomic Environment Is A Risk Factor For Malaria In Senegal ? A DHS Data Analysis of Malaria Trends From 2010 To 2016 In Senegal
Authors: Sougou Ndeye Mareme , Faye Adama , Leye Mamadou Makhtar Mbacké , Bassoum Oumar , Seck Ibrahima 
Published: July 2021

Abstract
Introduction Analysis of the evolution of malaria will help address the determinants of malaria elimination in this country. The aim of this study is to analyze the evolution of malaria in Senegal from 2010 to 2016.MethodsThis article uses data from the Senegalese Demographic and Health Surveys (for 2010-2011, 2012-2013, 2014, 2015 and 2016. To assess the factors associated with the positivity of the RDT, a multivariate logistic analysis was conducted to account for the effect of confounding factors. Adjusted odds ratios were calculated with their 95% confidence intervals. The dependent variable was the result of the Malaria rapid diagnostic test. ResultsThe malaria prevalence rate varies from 3.01% in 2010 to 0.87% in 2016. The risk factors associated with the evolution of malaria were the age of the child (4 years (aOR=1.82 [1.14-2.89]) and 5 years (aOR=1.98 [1.21-3.25]). The richest wealth quintile was a protective factor against malaria with aOR=0.02 [0-0.18]. Other protective factors against malaria were the construction characteristics of the houses. These are houses with improved wall and roof materials with aOR 0.45 [0.24-0.85] and 0.48 [0.25-0.93] respectively.Conclusion Factors associated with the evolution of malaria in Senegal are children’s age, level of wealth of the household and type of dwelling in the house. It should be noted that the level of development of countries, by influencing better living conditions for communities, remains an important prerequisite for the elimination of malaria in the African sub region and in Senegal in particular.
Heavily Calcified Gastrointestinal Stromal Tumour of Stomach: A Diagnostic Dilemma
Authors: Har Bappaditya , Kapoor Vinay Kumar , Behari A 
Published: July 2021

Abstract
Gastrointestinal stromal tumours (GIST) are the most common mesenchymal tumour of gastrointestinal tract and the stomach being the most commonly involved organ. Focal calcification may be seen in GIST but prominent or heavy calcification is rare. Gastric mass with prominent calcification on imaging may create a diagnostic dilemma. We present a rare case of gastric GIST with heavy calcification in a 55 years old female presenting with abdominal lump. Computed tomography (CT) showed a large heterogenous juxta gastric mass with solid-cystic component with heavy calcification. She underwent laparotomy and en-bloc gastric sleeve resection with the mass. Microscopic examination showed tumour with spindle cell and calcification with mitotic index of 6/50 High power field. Immunoreactivity with Vimentin, CD34 and DOG 1 confirmed diagnosis of GIST. Dystrophic calcification of necrotic or degenerative tissue is thought to be cause of calcification in GIST. Very few cases of heavily calcified GIST have been reported in literature, our case is of interest because presence of solid cystic component and a huge size ~ 14 cm (longest diameter).
Power Consumption Characteristics Research on Mobile System of Electrically Driven Large-Load-Ratio Six-Legged Robot
Authors: Zhuang Hong-Chao , Wang Ning , Gao Hai-Bo , Deng Zong-Quan 
Published: July 2021

Abstract
To research the power consumption characteristics of mobile system of an electrically driven large-load-ratio six-legged robot with engineering capability is beneficial to speed up it toward practicability. Based on the configuration and walking modes of robot, the mathematical model of the power consumption of mobile system is set up. In view of the tripod gait is often selected for the six-legged robots, the simplified power consumption model of mobile system under the tripod gait is established by means of reducing the dimension of the robot's statically indeterminate problem and constructing the equal force distribution. Then, the power consumption of robot mobile system is solved under different working conditions. The variable tendencies of the power consumption of robot mobile system are respectively obtained with changes in the rotational angles of hip joint and knee joint, body height, and span. The articulated rotational zones and the ranges of body height and span are determined under the lowest power consumption. According to the walking experiments of prototype, the variable tendencies of the average power consumption of robot mobile system are respectively acquired with changes in the duty ratio, body height, and span. Then, the feasibility and correctness of theory analysis are verified in the power consumption of robot mobile system. The proposed analysis method in this paper can provide a reference on the lower power research of the large-load-ratio multi-legged robots.
Composition and Thrombolytic Susceptibility of Ex Vivo Venous Thromboembolism
Authors: Hendley Samuel A. , Dimov Alexey , Bhargava Aarushi , Snoddy Erin , Mansour Daniel , Afifi Rana O. , Wool Geoffrey D. , Zha Yuanyuan , Sammet Steffen , Lu Zheng Feng , Ahmed Osmanuddin , Paul Jonathan D , Bader Kenneth B 
Published: July 2021

Abstract
Venous thromboembolism is a significant source of morbidity and mortality worldwide. Catheter-directed thrombolytics is the primary treatment used to relieve critical obstructions, though its efficacy varies based on the thrombus composition. Non-responsive portions of the specimen often remain in situ, which prohibits mechanistic investigation of lytic resistance or the development of diagnostic indicators for treatment outcomes. In this study, thrombus samples extracted from venous thromboembolism patients were analyzed ex vivo to determine their histological structure, lytic susceptibility, and imaging characteristics. A wide range of thrombus morphologies were observed, with a dependence on age and etymology of the specimen. The lytic inhibitor PAI-1 was present in samples, and may contribute to the lytic resistance of venous thrombi. Finally, a correlation was observed between the lytic response of the sample and its magnetic microstructure assessed with a quantitative MRI sequence. These findings highlight the myriad of changes in venous thrombi that may promote lytic resistance, and imaging metrics that correlate with treatment outcomes.
Seismic Behavior of RC Moment Resisting Structures with Concrete Shear Wall under Mainshock-aftershock Seismic Sequences
Authors: Soureshjani Omid Karimzade , Massumi Ali 
Published: July 2021

Abstract
A structure may subject to several aftershocks after a mainshock. In many seismic design provisions, the effect of the seismic sequences is not directly considered or underestimated. This paper studies the seismic behavior of RC moment-resisting structures with concrete shear wall under seismic sequences. Two three-dimensional structures of short and medium height were designed and analyzed. The former models were studied under a group of real mainshock-aftershock seismic sequences. The models were loaded and designed according to the fourth edition of the Iranian seismic code of standard no. 2800 and ACI-318 respectively. Furthermore, the non-linear dynamic time-history finite element analysis of models was performed via the explicit method. The parameters of maximum displacement, inter-story drift ratio, residual displacement, and finally the effect of the ratio of aftershock acceleration to mainshock acceleration were investigated and assessed. Due to the high lateral stiffness of shear walls, parallel with the complete elastic behavior, aftershocks cause no growth in inter-story drift ratio and relative displacement in the short structure model. In contrast, compared to the structure under the solely mainshock, the medium height structure model under seismic sequences showed significant growth in the amount of relative displacement (even more than 50% growth), inter-story drift ratio, plastic strain, and residual displacement (almost 30% growth). Furthermore, unlike the moment-resisting frame structures, models showed no significant growth in the drift ratio with the height. Assessments indicated that the ratio of aftershock to mainshock acceleration is a determinative parameter in structural behavior under seismic sequences.