bluuebunny/crossref_metadata_2025_split huggingface dataset:
Prognostic role of DFNA5 in head and neck squamous cell carcinoma revealed by systematic expression analysis
Authors: Liu Zhiguo , Liu Hongyan , Dong Qian , Li Hongyu , Zhang Bin , Liu Yufeng , Zhong Limei , Tang Haikuo 
Published: August 2021

Abstract
Background
The gasdermin E gene (GSDME, also known as DFNA5) is mutated in familial aging-related hearing loss. Recent studies have also revealed that the expression of DFNA5 is suppressed in many cancer types; however, little is known about the function of DFNA5 in head and neck squamous cell carcinoma (HNSCC). Accordingly, the aim of the present study was to evaluate the expression of DFNA5 and explore its prognostic value in HNSCC.

Result
We used a set of bioinformatics tools, including Oncomine, TIMER, TISIDB, cBioPortal, and GEPIA, to analyze the expression of DFNA5 in patients with HNSCC from public databases. Kaplan-Meier plotter was used to evaluate the potential prognostic significance of DFNA5. DFNA5 mRNA levels were significantly higher in HNSCC tissues than in normal tissues, and high DFNA5 expression was correlated with worse survival. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analyses showed that DFNA5 expression has a strong positive correlation with cell adhesion and the integrin signaling pathway, whereas its expression was negatively correlated with the levels of infiltrating B cells (cor = − 0.223, P = 8.57e-07) and CD8 T cells (cor = − 0.223, P = 2.99e-07).

Conclusion
This study demonstrates that DFNA5 expression has prognostic value for HNSCC patients. Moreover, these results suggest that regulation of lymphocyte infiltration is the mechanism underlying the function of DFNA5 in HNSCC.
Physical growth and neurodevelopment during the first year of life: a cohort study of the Japan Environment and Children’s Study
Authors: Sanefuji Masafumi , Sonoda Yuri , Ito Yoshiya , Ogawa Masanobu , Tocan Vlad , Inoue Hirosuke , Ochiai Masayuki , Shimono Masayuki , Suga Reiko , Senju Ayako , Honjo Satoshi , Kusuhara Koichi , Ohga Shouichi ,   Japan Environment and Children’s Study Group, Kamijima Michihiro , Yamazaki Shin , Ohya Yukihiro , Kishi Reiko , Yaegashi Nobuo , Hashimoto Koichi , Mori Chisato , Ito Shuichi , Yamagata Zentaro , Inadera Hidekuni , Nakayama Takeo , Iso Hiroyasu , Shima Masayuki , Kurozawa Youichi , Suganuma Narufumi , Kusuhara Koichi , Katoh Takahiko 
Published: August 2021

Abstract
Background
The association between a slower physical growth and poorer neurodevelopment has been established in infants born preterm or small for gestational age. However, this association is inconsistent in term-born infants, and detailed investigations in infancy, when intervention is most beneficial for improving outcomes, are lacking. We therefore examined this association separately by sex during the first year of life in term-born infants.

Methods
Using data collected until children reached 12 months old in an ongoing prospective cohort of the Japan Environment and Children’s Study, we analyzed 44,264 boys and 42,541 girls with singleton term-birth. The exposure variables were conditional variables that disentangle linear growth from weight gain relative to linear growth, calculated from the length and weight at birth and 4, 7 and 10 months old. Neurodevelopmental delay was identified using the Japanese-translated version of Ages & Stages Questionnaires, third edition.

Results
A reduced risk of neurodevelopmental delay at 6 months old was observed in children with a higher birth weight (adjusted relative risks [aRRs]: 0.91 and 0.93, 95 % confidence intervals [95 % CIs]: 0.87–0.96 and 0.88–0.98 in boys and girls, respectively) and increased linear growth between 0 and 4 months old (aRRs: 0.85 and 0.87, 95 % CIs: 0.82–0.88 and 0.83–0.91 in boys and girls, respectively). A reduced risk at 12 months was found in children with an increased linear growth between 0 and 4 months (aRRs: 0.92 and 0.90, 95 % CIs: 0.87–0.98 and 0.84–0.96 in boys and girls, respectively), boys with an increased relative weight gain between 0 and 4 months (aRR: 0.90, 95 % CI: 0.84–0.97), and girls with a higher birth weight (aRR: 0.89, 95 % CI: 0.83–0.96).

Conclusions
These results suggest that a slow physical growth by four months old may be a predictor of neurodevelopmental delay during infancy.
Analysis of tweets on toothache during the COVID-19 pandemic using the CrystalFeel algorithm: a cross-sectional study
Authors: Altan Halenur , Coşgun Alem 
Published: August 2021

Abstract
Background
Reasons such as the lack of information on the COVID-19 disease, lack of proven treatment for it, uncertainty about the duration of the pandemic, or social isolation affect people’s mental health. This study aimed to analyze the emotional type and intensity in tweets on toothache posted during the COVID-19 pandemic.

Methods
Using the Twitter Search API, we collected tweets in English associated with the keywords “Corona, Toothache” “Corona, Tooth, Pain” “Corona, Dental Pain” “Covid-19, Toothache” “Covid-19, Tooth, Pain” and “Covid-19, Dental Pain” posted between March 11, 2020 and June 30, 2020 all around the world. After the relevant inclusion and exclusion criteria were applied, 426 posts were selected and analyzed using the CrystalFeel algorithm, a sensitivity analytical technology with proven accuracy. The chi-square test (SPSS v23, IBM) was used to compare emotions and emotional intensities according to the words used.

Results
It was determined that 80.3% of the participants experienced fear and 61.7% had a negative emotional intensity. There was no statistically significant difference between the distributions of emotions according to the words without time distinction (p = 0.136). There was a statistically significant difference between the distributions of emotional intensity according to the words without time distinction (p = 0.006). The keyword “Corona, Toothache” was used the most frequently by 30.8% of the participants.

Conclusions
This study is the first to analyze the emotional reactions of individuals who experienced toothaches during the COVID-19 pandemic using the CrystalFeel algorithm. Monitoring the social media posts of individuals experiencing toothache during the pandemic will help reduce fear and anger emotions and design public information messages that are compatible with the target group’s needs.
Large differences in the organization of palliative care in nursing homes in six European countries: findings from the PACE cross-sectional study
Authors: Honinx E. , Van den Block L. , Piers R. , Onwuteaka-Philipsen B. D. , Payne S. , Szczerbińska K. , Gambassi G. , Kylänen M. , Deliens L. , Smets T. ,   on behalf of PACE, Gatsolaeva Yuliana , Miranda Rose , Pivodic Lara , Tanghe Marc , van Hout Hein , Pasman Roeline H. R. W. , Oosterveld-Vlug Mariska , Piers Ruth , Wichmann Anne B. , Engels Yvonne , Vernooij-Dassen Myrra , Hockley Jo , Payne Sheila , Leppäaho Suvi , Barańska Ilona , Pautex Sophie , Bassal Catherine , Mammarella Federica , Mercuri Martina , Rossi Paola , Segat Ivan , Stodolska Agata , Adang Eddy , Andreasen Paula , Kuitunen-Kaija Outi , Moore Danni Collingridge , Pac Agnieszka , Kijowska Violetta , ten Koppel Maud , van der Steen Jenny T. , de Paula Emilie Morgan 
Published: August 2021

Abstract
Background
To be able to provide high-quality palliative care, there need to be a number of organizational structures available in the nursing homes. It is unclear to what extent such structures are actually present in nursing homes in Europe. We aim to examine structural indicators for quality of palliative care in nursing homes in Europe and to evaluate the differences in terms of availability of and access to palliative care, infrastructure for residents and families, multidisciplinary meetings and quality improvement initiatives.

Methods
A PACE cross-sectional study (2015) of nursing homes in Belgium, England, Finland, Italy, the Netherlands and Poland. Nursing homes (N = 322) were selected in each country via proportional stratified random sampling. Nursing home administrators (N = 305) filled in structured questionnaires on nursing home characteristics. Organization of palliative care was measured using 13 of the previously defined IMPACT structural indicators for quality of palliative care covering four domains: availability of and access to palliative care, infrastructure for residents and families, multidisciplinary meetings and quality improvement initiatives. We calculated structural indicator scores for each country and computed differences in indicator scores between the six countries. Pearson’s Chi-square test was used to compute the p-value of each difference.

Results
The availability of specialist palliative care teams in nursing homes was limited (6.1–48.7%). In Finland, Poland and Italy, specialist advice was also less often available (35.6–46.9%). Up to 49% of the nursing homes did not provide a dedicated contact person who maintained regular contact with the resident and relatives. The 24/7 availability of opioids for all nursing home residents was low in Poland (37.5%).

Conclusions
This study found a large heterogeneity between countries in the organization of palliative care in nursing homes, although a common challenge is ensuring sufficient structural access to specialist palliative care services. Policymakers and health and palliative care organizations can use these structural indicators to identify areas for improvement in the organization of palliative care.
The urban–rural differential in the association between household wealth index and anemia among women in reproductive age in Ethiopia, 2016
Authors: Abate Teshager Weldegiorgis , Getahun Biruk , Birhan Mekuriaw Mesfin , Aknaw Getasew Mulatu , Belay Sefealem Assefa , Demeke Dessalegn , Abie Dagninet Derebe , Alemu Adela Memberu , Mengiste Yirga 
Published: August 2021

AbstractBackgroundAnemia is more prevalent among women, and it is a moderate public health problem in Ethiopia. The wealth status and place of residence of a woman have implications on the intervention of anemia. Studies that examined the relationship between women’s wealth index status and residency in Ethiopia are scarce. We aimed to identify the urban–rural differential in the association between household wealth index and anemia among women of childbearing age in Ethiopia.MethodA cross-sectional design was employed with a nationally representative sample of 14,100 women aged 15–49-year-old from the Ethiopian demographic and health survey conducted in 2016. We used the two-stage sampling method to select the sample size. The primary outcome was anemia in women of childbearing age. A hemoglobin level of below 11 g/dl for pregnant women and 12 g/dl for non-pregnant women was the indicator of anemia. Using a three-level random intercept model to explore associated factors at the individual and household levels quantified the observed and unobserved variations between household wealth index and residence on anemia.ResultsWomen belonging to a lower household wealth index category were more anemic (29.6%) than those middle and above wealth index categories. Women who lived in rural areas (25.5%) were prone to anemia than those who lived in urban areas (17.5%). The odds of anemia were significantly higher in women of the low household wealth category who living in rural compared to women of the middle and above household wealth category who living in urban (AOR = 1.37, 95% CI 1.14–1.65,P < 0.001).ConclusionIn this study, anemia is more common among women who live in rural with the low house wealth category.Therefore, novel public health interventions should target women who live in rural areas with the lowest household wealth status.
Effects of different feeding regimens with protease supplementation on growth, amino acid digestibility, economic efficiency, blood biochemical parameters, and intestinal histology in broiler chickens
Authors: Amer Shimaa A. , Beheiry Rasha R. , Abdel Fattah Doaa M. , Roushdy Elshimaa M. , Hassan Fardos A. M. , Ismail Tamer Ahmed , Zaitoun Noha M. A. , Abo-Elmaaty Azza M. A. , Metwally Abdallah E. 
Published: August 2021

Abstract
Background
This study was conducted to estimate the impacts of using varied feeding regimens with or without protease supplementation on the growth performance, apparent amino acid ileal digestibility (AID%), economic efficiency, intestinal histology, and blood biochemical parameters of broiler chickens. Three hundred one-day-old chicks (Ross 308 broiler) were randomly allotted to a 3 × 2 factorial design. The experimental design consisted of three feeding regimens; FR1: a recommended protein SBM diet, FR2: a low-protein SBM diet, and FR3: a low-protein diet with the inclusion of 5% DDGS and 5% SFM, with or without protease supplementation (250 mg/kg).

Results
Increased feed intake and feed conversion ratio were observed in the FR3 treatment during the starter stage and decreased body weight and body weight gain during the grower stage. However, there was no significant effect of the different feeding regimens, protease supplementation, or interaction on the overall performance. The economic value of diets also remained unaffected by the different feeding regimens, protease supplementation, or interaction. Protease supplementation resulted in lowering the AID% of tryptophan and leucine. Reduced AID% of methionine was evident in the FR2 + VE and FR3 − VE treatments. Histological findings substantiated the FR3 treatment mediated a decrease in the duodenal and jejunal villous height (VH), jejunal villous width (VW), and ileal VW, whereas, increase in the ileal crypt depth (CD). The FR2 + VE treatment reduced the VH:CD ratio in the duodenum. The duodenal CD and the jejunal goblet cell count were reduced as a consequence of protease supplementation. The FR3 + VE treatment documented a rise in duodenal CD, while an increase in the jejunal goblet cell count was observed in the FR3 − VE treatment. The FR3 treatment enhanced the IgM serum levels compared to the FR1 and FR2 treatments. IgM serum levels were also elevated following protease supplementation. FR3 + VE treatment increased IgM serum levels. The highest serum ALP was found in the FR3 treatment, whereas the lowest level was obtained in the FR2 treatment.

Conclusion
Low-protein SBM-based diets could be used without affecting the birds’ growth. Altered morphometric measures of the intestine and increased IgM and ALP levels indicated the low-protein SBM/DDGS-SFM diet-induced damage of the intestinal histoarchitecture and immune system of birds. These different diets and protease supplementation failed to affect economic efficiency positively.
Antiplasmodial, antimalarial activities and toxicity of African medicinal plants: a systematic review of literature
Authors: Tajbakhsh Elahe , Kwenti Tebit Emmanuel , Kheyri Parya , Nezaratizade Saeed , Lindsay David S. , Khamesipour Faham 
Published: August 2021

AbstractBackgroundMalaria still constitutes a major public health menace, especially in tropical and subtropical countries. Close to half a million people mainly children in Africa, die every year from the disease. With the rising resistance to frontline drugs (artemisinin-based combinations), there is a need to accelerate the discovery and development of newer anti-malarial drugs. A systematic review was conducted to identify the African medicinal plants with significant antiplasmodial and/or anti-malarial activity, toxicity, as wells as assessing the variation in their activity between study designs (in vitro and in vivo).MethodsKey health-related databases including Google Scholar, PubMed, PubMed Central, and Science Direct were searched for relevant literature on the antiplasmodial and anti-malarial activities of African medicinal plants.ResultsIn total, 200 research articles were identified, a majority of which were studies conducted in Nigeria. The selected research articles constituted 722 independent experiments evaluating 502 plant species. Of the 722 studies, 81.9%, 12.4%, and 5.5% were in vitro, in vivo,and combined in vitro and in vivo,respectively. The most frequently investigated plant species wereAzadirachta indica, Zanthoxylum chalybeum, Picrilima nitida,andNauclea latifoliameanwhileFabaceae, Euphorbiaceae, Annonaceae, Rubiaceae, Rutaceae, Meliaceae,andLamiaceaewere the most frequently investigated plant families. Overall, 248 (34.3%), 241 (33.4%), and 233 (32.3%) of the studies reported very good, good, and moderate activity, respectively.Alchornea cordifolia, Flueggea virosa, Cryptolepis sanguinolenta, Zanthoxylum chalybeum,andMaytenus senegalensisgave consistently very good activity across the different studies. In all, only 31 (4.3%) of studies involved pure compounds and these had significantly (p = 0.044) higher antiplasmodial activity relative to crude extracts. Out of the 198 plant species tested for toxicity, 52 (26.3%) demonstrated some degree of toxicity, with toxicity most frequently reported withAzadirachta indicaandVernonia amygdalina. These species were equally the most frequently inactive plants reported. The leaves were the most frequently reported toxic part of plants used. Furthermore, toxicity was observed to decrease with increasing antiplasmodial activity.ConclusionsAlthough there are many indigenous plants with considerable antiplasmodial and anti-malarial activity, the progress in the development of new anti-malarial drugs from African medicinal plants is still slothful, with only one clinical trial withCochlospermum planchonii(Bixaceae) conducted to date. There is, therefore, the need to scale up anti-malarial drug discovery in the African region.
Human-Biomonitoring derived exposure and Daily Intakes of Bisphenol A and their associations with neurodevelopmental outcomes among children of the Polish Mother and Child Cohort Study
Authors: Garí Mercè , Moos Rebecca , Bury Daniel , Kasper-Sonnenberg Monika , Jankowska Agnieszka , Andysz Aleksandra , Hanke Wojciech , Nowak Dennis , Bose-O’Reilly Stephan , Koch Holger M. , Polanska Kinga 
Published: August 2021

Abstract
Background
Bisphenol A (BPA) is an industrial chemical mostly used in the manufacture of plastics, resins and thermal paper. Several studies have reported adverse health effects with BPA exposures, namely metabolic disorders and altered neurodevelopment in children, among others. The aim of this study was to explore BPA exposure, its socio-demographic and life-style related determinants, and its association with neurodevelopmental outcomes in early school age children from Poland.

Methods
A total of 250 urine samples of 7 year-old children from the Polish Mother and Child Cohort Study (REPRO_PL) were analyzed for BPA concentrations using high performance liquid chromatography with online sample clean-up coupled to tandem mass spectrometry (online-SPE-LC-MS/MS). Socio-demographic and lifestyle-related data was collected by questionnaires or additional biomarker measurements. Emotional and behavioral symptoms in children were assessed using mother-reported Strengths and Difficulties Questionnaire (SDQ). Cognitive and psychomotor development was evaluated by Polish adaptation of the Intelligence and Development Scales (IDS) performed by trained psychologists.

Results
Urinary BPA concentrations and back-calculated daily intakes (medians of 1.8 μg/l and 46.3 ng/kg bw/day, respectively) were similar to other European studies. Urinary cotinine levels and body mass index, together with maternal educational level and socio-economic status, were the main determinants of BPA levels in Polish children. After adjusting for confounding factors, BPA has been found to be positively associated with emotional symptoms (β: 0.14, 95% CI: 0.022; 0.27). Cognitive and psychomotor development were not found to be related to BPA levels.

Conclusions
This study represents the first report of BPA levels and their determinants in school age children in Poland. The exposure level was found to be related to child emotional condition, which can have long-term consequences including social functioning and scholastic achievements. Further monitoring of this population in terms of overall chemical exposure is required.
Developmentally appropriate transitional care during the Covid-19 pandemic for young people with juvenile-onset rheumatic and musculoskeletal diseases: the rationale for a position statement
Authors: McDonagh Janet E. , Tattersall Rachel , Clinch Jacqui , Swan Joanne , Foster Helen E. , McCann Liza 
Published: August 2021

AbstractBackgroundThe importance of developmentally appropriate transitional care in young people with juvenile-onset rheumatic and musculoskeletal disease is well recognised. The Paediatric Rheumatology European Society (PReS) / European League Against Rheumatism (EULAR) Taskforce has developed international recommendations and standards for transitional care and a growing evidence base supports the positive benefits of such care. However, there is also evidence that universal implementation has yet to be realised. In 2020, against this background the COVID-19 pandemic arrived with significant impact on all our lives, young and old, patient, public and professional alike. The unfortunate reality of the pandemic with potential for unfavourable outcomes on healthcare provision during transition was acknowledged by the PReS working groups in a position statement to support healthcare professionals, young people and their caregivers.AimThe aim of this review is to present the literature which provides the rationale for the recommendations in the PReS Position Statement.SummaryThe following areas are specifically addressed: the prime importance of care coordination; the impact of the pandemic on the various aspects of the transition process; the importance of ensuring continuity of medication supply; the pros and cons of telemedicine with young people; ensuring meaningful involvement of young people in service development and the importance of core adolescent health practices such as routine developmental assessment psychosocial screening and appropriate parental involvement during transitional care.