bluuebunny/crossref_metadata_2025_split huggingface dataset:
Genome-Wide Study of the ABI3 Gene Family and Identification of Putative miRNA Targeting ABI3 Gene in Oryza Sativa ssp. Indica
Authors: Vashisth Apoorva , Singh Deepak Kumar , Chakraborty Navjyoti , Purty Ram Singh , Chatterjee Sayan 
Published: August 2021

Abstract
Rice is one of the important cereal crops mainly cultivated in Asia and its productivity is severely affected by drought stress. In response to drought stress, several genes are reported to be up-regulated or down-regulated in plants. Gene expression is negatively regulated by non-coding endogenous microRNAs post-transcriptionally either by mRNA degrading or translational silencing. In the past, single or multiple stress-responsive genes were over-expressed in order to generate drought-tolerant transgenic rice but with very little success. Recently, the development of transgenic plants by over-expressing transcription factors have received much attention because of their ability to regulate several genes. Abscisic Acid Insensitive 3 (ABI3) is a transcription factor, which is known to play a crucial role in mediating plant stress tolerance. Using the Ensembl plants database, we identified 83 putative OsABI3 genes in Oryza sativa Indica. Through in silico approach, five potential miRNAs i.e., ath-miR5021, csi-miR3948, osa-miRf11773-akr, osa-miRf12029-akr and ptc-miRf10053-akr that target OsABI3 genes were identified. Further, the expression of the selected ABI3 genes were analyzed in rice seedlings exposed to 15% PEG, using the RT-qPCR. In comparison to control, OsABI3 genes showed relatively enhanced expression when exposed to drought stress treatment. This indicates that OsABI3 genes may play important role in development and drought stress in rice seedlings.
Assessment of Genetic Stability on In Vitro And Ex Vitro Plants of Ficus Carica Var. Black Jack Using Issr and Damd Markers
Authors: Parab Ankita Rajendra , Lynn Chew Bee , Subramaniam Sreeramanan 
Published: August 2021

Abstract
In vitro propagation has been significant in producing a large number of genetically stable regenerated plants. Regenerated Ficus carica var. Black Jack plantlets were established using woody plant medium (WPM) supplemented with 20 µM 6-Benzylaminopurine (BAP) and 8 µM Indole-3-acetic acid (IAA) under different light treatments such as normal fluorescent white light (60 µmol.m− 2.s− 1), and four different LED spectra, white (400– 700nm), blue (440nm), red (660nm) and blue + red (440nm + 660nm). Genetic stability analysis was performed on the in vitro and ex vitro plants of Ficus carica var. Black Jack. Ten (10) primers of each ISSR and DAMD molecular marker were used to assess the genetic stability of the eight (8) samples of Ficus carica var. Black Jack, acquired over two years. The findings of this study revealed that inter simple sequence repeats (ISSR) and directed amplification of minisatellite DNA (DAMD) markers (DNA primers) are efficient in determining the polymorphism and monomorphism percentage among the in vitro and ex vitro samples of Ficus carica var. Black Jack. ISSR markers showed 97.87% of monomorphism whereas DAMD markers showed 100% monomorphism. Polymorphism of 2.13% was observed for the UBC840 ISSR – DNA primer which was negated under the genetic similarity index analysis for the eight samples. It is recommended that genetic stability analysis should be performed for long-term maintenance of micropropagated plants.
Tensile behavior of a Brazilian Disk Containing non-persistent Joint Sets Subjected to Diametral Loading: An Experimental Investigation
Authors: Asadizadeh Mostafa , Shakeri Jamshid , Babanouri Nima , Rezaei Mohammad 
Published: August 2021

Abstract
Structural defects are part of the inherent characteristics of rock masses. They can be found in the form of fishers, joints, and beddings and can be divided into persistent or non-persistent one. The coalescence of non-persistent cracks may lead to the formation of persistent joints under the tensile stress field, leading to instability of rock mass. The mechanical behavior of non-persistent jointed disks under tensile stress has essential implications for rock engineering structures. In this paper, concrete Brazilian disks containing open non-persistent joints were constructed and subjected to diametral loading to investigate the effect of this kind of joint parameters on the tensile strength and stiffness of disks. The effect of some parameters, such as joint continuity factor (the relationship between joint length and rock bridge length), bridge angle, joint spacing, and loading direction with respect to joint angle were investigated to estimate the tensile strength and stiffness as well as failure pattern. The results of experiments revealed that the tensile strength, stiffness, and failure pattern of Brazilian disks are highly affected by non-persistent pre-existing crack parameters. The increase of joint continuity factor and loading direction leads to an increase in tensile strength and a decrease in stiffness. However, when bridge angle and spacing increase tensile strength rises, and the former decreases stiffness while the latter results in its reduction. Finally, all the parameters significantly affect the failure pattern, and some failure patterns such as step-path failure, splitting, or sliding may occur as a function of non-persistent joint parameters.
Immunohistochemical Characterization of Cell of Origin in Diffuse Large B-cell Lymphoma and its Correlation with the Double Expressor Phenotype: A Retrospective Study in a Tertiary Cancer Care Centre
Authors: Chakrabarti suparno , aiyer Hema MALINI , sharma gaurav , jaiswal sarita rani , prachi Prachi 
Published: August 2021

Abstract
Background – Diffuse Large B Cell Lymphoma (DLBCL) is the most common type of Non- Hodgkin Lymphoma (NHL) and is categorized into the Germinal Centre B-cell (GCB) and Activated B-cell (ABC/ Non-GCB) subtypes as per the Cell-of-origin (COO) model with the help of Gene Expression Profiling/ Immunohistochemistry. The non -GCB subtype has been found to correlate with the Double Expressor (DE) phenotype (i.e. co-expression of bcl-2 and c-myc by IHC) and this correlation was substantiated and proven to be statistically significant in the present study.Results- A retrospective study of 50 patients was carried out with the help of archival material filed in the Department of Anatomic Pathology at Dharamshila Narayana Superspeciality Hospital from 1st January 2019 to 30th June 2020. The study cohort was divided into two Groups – Group-A with nodal presentation and Group -B with extranodal presentation. By using the Hans and Choi IHC algorithms, the cases were categorized into the GCB and non – GCB subtypes in both the groups. The DE phenotype was determined in each case by the co-expression of c-myc (>40%) and bcl-2 (>50%) by IHC.Conclusion – Due to the heterogeneity inherent in DLBCL, prediction of the Double Expressor phenotype and the COO by IHC is a sensitive tool and helps in the prognostication and therapeutic triage of patients. Hence in all cases diagnosed as DLBCL, a detailed morphological and IHC work up is mandatory to determine prognosis and possibly tailor therapy according to cell of origin and double expressor phenotype.
High Rate of Epidermal Growth Factor Receptor-Mutated Primary Lung Cancer in Patients with Primary Breast Cancer
Authors: Zeng Tianyu , Xu Hai , Liu Yincheng , Yang Yiqi , Yang Fan , Yang Mengzhu , Sun Chunxiao , Bao Shengnan , Hua Yijia , Yan Xueqi , He Yaozhou , Wang Xi , Jiang Mengping , Liang Yan , Huang Xiang , Li Jun , Fu Ziyi , Li Wei , Yin Yongmei 
Published: August 2021

Abstract
Background

With increased survival in breast cancer, resulting from advances in treatment, patients incur the possibility of subsequent primary malignancies, especially lung cancer. The aim of this study was to assess the frequency of CT-detected pulmonary ground-glass nodules and lung cancer following breast cancer diagnosis, the associations between breast cancer and lung cancer, the pathological features of double primary cancer, and the status of epidermal growth factor receptor (EGFR) mutations in second primary lung cancer.
Methods

Clinical data from more than 8000 individuals who were diagnosed with primary breast cancer at Jiangsu Province Hospital (Jiangsu, China) between January 2008 and December 2020 were retrospectively analyzed.
Results

Of the 8048 patients, 55 (0.7%) were diagnosed with a second primary lung cancer, which accounted for approximately 14.8% of the pulmonary ground-glass opacity (GGO) detected. The incidence was higher than in the general female population (standardized incidence ratio 1.4 [95% confidence interval (CI): 1.25–1.55]). Patients who experienced a second primary lung cancer exhibited a significantly higher rate of EGFR mutation (78.5%) than those with lung adenocarcinoma alone, with most exhibiting low-grade malignancy, older age, estrogen receptor negativity, low Ki67, and no lymph node metastasis.
Conclusions

Breast cancer patients, especially those with low-grade malignancy, were at high risk for developing primary lung cancer. For isolated GGO in patients with high-risk factors, clinicians should insist on close follow-up. Furthermore, EGFR may play an important role in primary lung adenocarcinomas and breast cancer.
EZR Promotes Pancreatic Cancer Proliferation and Metastasis by Activating FAK/AKT Signaling Pathway
Authors: Xu Jian , Zhang Wei 
Published: August 2021

Abstract
Background: As a member of the ERM (ezrin-radixin-moesin) protein family, EZR has been recognized as a regulator of adhesion signal pathways by researchers. Moreover, EZR was thought to play irreplaceable roles in invasion and metastasis of versatile cancers. In this study, we managed to undermine the effect of EZR on proliferation and metastasis in pancreatic cancer (PC).Methods: To analyze the impact of EZR expression on overall survival and free diseases survival of PC patients, we screened abnormally expressed EZR in PC using the Gene Expression Omnibus database(GEO database) and The Cancer Genome Atlas (TCGA) database. Following, Gene Ontology (GO)-based functional analysis and Gene set enrichment analysis (GSEA) was performed to predicate the possible biological processes in which EZR were involved. The clinicopathological characteristics and prognosis of PC patients were analyzed according to clinical data. Further, immunohistochemistry, western blotting and real time PCR analysis were conducted to analyze the expression level of EZR in PC and paired paracancerous tissues. The effect of EZR on proliferation of PC cell lines were detected by Cell Counting Kit-8 assay, and meanwhile, Transwell assay was performed to detect the effect of EZR on invasion and migration of PC cell.Result: EZR exhibited higher expression level in pancreatic cancer tissues and cell than paracancerous tissues and cell, and its expression level was positively correlated with poor overall survival and diseases-free survival in PC patients. CCK8 assay indicated that EZR facilitated the proliferation of PC cells, meanwhile, Transwell assay showed that EZR promoted the migration and invasion of PC cells. The GO analysis predicated that EZR was involved in biological processes including cell adhesion, ameboidal-type cell migration, cell junction assembly. Through GSEA analysis, pancreatic cancer pathway, and the adhesion junction pathway were screened as the mostly enriched pathways in EZR-regulated pathological process. The inhibition of EZR suppressed proliferation and migration of PC cells. Western blot experiment revealed a positive correlation between EZR and FAK, the proliferation invasion and migration ability of PC cells were significantly decreased after knockdown of EZR.Conclusion: Our finding revealed EZR accelerated the progression of PC via FAK/AKT signaling pathway.
RPS27A is a Poor Prognostic Predictor for Hepatocellular Carcinoma via Interacting with METTL3-Mediated RNA Modifications
Authors: Liu Huiying , Zhu Jianjun , Kou Xiaoxia , Guo Lingling , Zhang Hongjuan , Chen Xiaofeng , Qiu Jinrong 
Published: August 2021

Abstract
Background: Increasing evidence has pointed to the involvement of RNA modifications in the pathogenesis of human cancers. However, they are rarely studied in hepatocellular carcinoma (HCC). Method: We summarized multiple types of RNA modification-related genes (RMRGs) from public references, and identified differentially expressed RMRGs (DEGs) between HCC tissues and matched normal samples, where their genetic variation were then investigated. The potential hub genes in the protein-protein interaction (PPI) network constructed by co-expression genes of RMRGs were recognized and verified in METTL3-knockdown HCC cell lines by quantitative PCR assay.Results: Seventy-six RMRGs, including six writers, seven readers, and seven erasers, were collected, of which 34 were identified and validated as DEGs. YTHDC2 exhibited the highest mutation rate, while ADAT2 showed widespread deletions. High correlations were observed between the expressions of 34 RMRGs. The PPI network constructed by 1080 co-expression DEGs related to RNA regulations consisted of 513 nodes and 11557 edges, with RPS27A presented the most directed edges and maximum closeness centrality. Patients with high expression of RPS27A showed worse overall survival (P < 0.01) and disease-free survival (P = 0.019). Moreover, RPS27A was found upregulated on high-risk metastatic and recurrent HCC tissues. Quantitative PCR assay indicated that RPS27A was significantly decreased in cancer cell lines when METTL3 was knocked down. Conclusions: Remarkable differences were observed for RNA modifications between HCC and normal samples, and RPS27A could be a poor prognostic predictor for HCC via interacting with METTL3-mediated RNA modifications.
Relationships between plant community productivity and diversity vary with trampling disturbance in an Inner-Mongolian steppe
Authors: Liu Yinzhan , Zhang Luna , Han Yangyang , Chen Anqun , Miao Renhui , Chen Ji , Wang Dong 
Published: August 2021

Abstract
The relationship between diversity and productivity of plant communities is an important issue in grassland restoration. However, the degree to which this relationship varies during the restoration stage after trampling disturbance is not clear. Here, we conducted a five-year study in a steppe after 4-year trampling to detect restoration patterns of plant community and investigate variation in diversity-productivity relationships. Our results showed that community cover, abundance, height, and productivity recovered quickly after the trampling disturbance ceased. However, the recovery of diversity was slower than biomass in the steppe. In addition, grass, annual, and biennial recovery was more rapidly than the recovery of forbs in the steppe. Moreover, following the restoration process, the positive correlation between productivity and diversity was decoupled, and a negative correlation between productivity and diversity developed. Our finding provides the key evidence for the asynchronous relation between productivity and diversity, and reveals that grass restored more rapidly than forbs in plant community restoration after disturbance. This study indicates that the trade-off between plant community structure and function can vary with the restoration process, and implicates that future modeling and experimental studies should focus on the different responses of productivity and diversity in plant community restoration.
Failure Analysis of A Welded Mild Steel Shaft Under Tensile Loading Condition
Authors: Hussain Arshad , Hussain Fida , Sattar Abdul , Hussain Tanweer , Bakhsh Qadir , Ahmed Muhammad 
Published: August 2021

Abstract
Welding is a process of permanently joining two or more parts by the application of heat and (or) pressure and is the widely used process in fabrication, maintenance and repairs of parts and structures. Almost 70 percent of all manufactured products are made with the skills of welders. In the field of Mechanical Engineering the modern welding trends and advanced products are made with the skills of welders. In the field of Mechanical Engineering the modern welding trends and advanced welding process has been contributing toward advancement, a study has been given greater priority of failures analysis of welding joints and various structural and technical aspects have an impact on fracture characteristics of welded joints. The welded joints and various structural aspects have an impact on fracture characteristics of welded joints. The objectives of this work were to investigate the tensile behavior of welded mild steel shaft to analyzing the failure analysis of welded joints for that purpose step joint, lap joint, double v joint, double v pin joint and double j joint specimens of standard size were developed. Each specimen was machined, welded and finished at its center. A tensile test was performed on all the welded shaft of mild steel. Tensile test has been carried out by Universal Testing Machine, after testing the specimens, it was found that the welded shaft with Lap Joints bears the Maximum Tensile Strength.
Multiobjective Site Selection Model for Emergency Shelter Facilities in Urban Areas
Authors: Yen Kuo-Chi , Chang Weid , Shih Wu-Chiao 
Published: August 2021

Abstract
Industrial and economic development is primarily applied to densely populated urban areas. If a sudden disaster occurs in such areas, the consequences can be severe. Shelter facility location affects the implementation of postdisaster relief work. This study explored residents’ perceived utility of evacuation time, their risk utility for road blocking, and the cost factors associated with constructing shelter facilities in the context of governance. A location model for emergency shelter facilities in cities was established on the basis of the aforementioned factors. Because the resolution of the random-weighted genetic algorithm (RWGA) is susceptible to influence from random weights, a robustness random-weighted method (RRWM) was developed. The validity and feasibility of the location model were examined through numerical analysis. Finally, the convergence of the RRWM was analyzed and compared with that of the RWGA and a single-objective genetic algorithm. The results revealed that the proposed algorithm exhibited satisfactory performance and can assist in evaluation and decision-making related to the selection of urban shelter facility locations.
History of Atopy Confers Improved Outcomes In IDH Mutant And Wildtype Low-Grade Glioma
Authors: Jaman Emade , Zhang Xiaoran , Sandlesh Poorva , Habib Ahmed , Allen Jordan , Saraiya Raj G. , Zinn Pascal O. 
Published: August 2021

Abstract
Purpose:A history of atopy or allergy has been shown to be protective against the development of glioma, however the effect of atopy on patient outcomes, especially in conjunction with the survival benefit associated with IDH mutation, has not yet been investigated, and is the focus of the study we present here. Methods:Low grade glioma (LGG) data from the TCGA was downloaded, along with IDH, TERT, 1p/19q and ATRX mutational status and genetic alterations. History of asthma, eczema, hay fever, animal, or food allergies, as documented in TCGA, was used to determine patient atopy status. Patients with missing variables were excluded from the study. Results:374 LGG studies were included. Patients with a history of atopy demonstrated longer overall survival (OS) compared to those without (145.3 vs. 81.5 months, p=00.0195). IDH mutant patients with atopy had longer OS compared those without atopy (158.8 vs. 85 months, p=0.035). Multivariate cox regression analysis demonstrated that the effects of atopy on survival were independent of IDH and histological grade, (p=0.002, HR 0.257, 95% 0.109-0.604), (p=<0.001, HR 0.217, 95% 0.107-0.444), and (p=0.004, HR 2.72, 95% 1.373-5.397), respectively. In terms of treatment outcomes, patients with atopy did not differ in treatment response compared to their counterpart. Pathway analysis demonstrated an upstream activation of the BDNF pathway (p=0.00027). Conclusion:A history of atopy confers a survival benefit in patients with diffuse low-grade glioma. Activation of the BDNF pathway may drive the observed differences.
Heavy Metal Contamination and Ecological Risk Assessment in Fluvial Sediment of San Juan –Taxco River System in Mining Region of Taxco Guerrero, Mexico
Authors: Sanchez Edith R. Salcedo , Morales Manuel Martínez , Mart Juan Manuel Esquivel , Mendoza Oscar Talavera 
Published: August 2021

Abstract
The hydrological system of San Juan-Taxco Rivers in located inside of one of the oldest and major mining district in Mexico. Several communities in the area use the rivers water along its flow for domestic water supply and crop irrigation. Sediment is an essential, integral and dynamic part of river basins, in polluted environments these act as sink of heavy metals and as a source of contaminants on the fluvial system. The management and sustainability of sediment should be assessed and secured to achieve good ecological status of the basins, for this task, approaches as ecological risk identification and geochemical indices are being used.Superficial sediment samples were collected in San Juan-Taxco river system. The results demonstrated that the degree of pollution from thirteen heavy metals and metalloids studied decreases in the following sequence: Cd> Zn > Pb > Cu > As > B > Mn > Ni > Fe > Co > Ba > Al and Cr. Cd made the most dominant contribution. Geochemical indices revealed important external anthropogenic influences in the rivers. The geochemical indices indicated very high enrichment for As, Cu, Pb and Zn, and extremely high for Cd in the three-rivers. The calculation of Pollution Load Index (PLI) showed in Cacalotenago River and in Taxco River are the sites with the highly contaminated sediments. PLI values were very high in all the samples sites due mining tailings erosion, wastewater and agriculture run off. Cd, Zn, Pb, Cu and As were the main potential risk elements that will cause harmful biological effects in the riverine environment.
hAMSC-CM Attenuates Paraquat-Induced A549 Cell Damage by Reducing Endoplasmic Reticulum Stress and Oxidative Stress
Authors: Liao Futuan , Gong Liming , Jia Lijing , Wang Jianhong , Liu Tongying , Huang Tingting , Liang Jing , zhou manhong 
Published: August 2021

Abstract
Acute paraquat (PQ) poisoning results in severe acute lung injury and pulmonary fibrosis, and there is no specific antidote; thus, the mortality rate of PQ poisoning is extremely high. The mechanism of poisoning may be associated with endoplasmic reticulum stress, oxidative stress damage and organ/tissue inflammation. Recent studies have reported that human amnion-derived mesenchymal stem cells (hAMSCs) secrete a variety of cytokines, and that hAMSC-conditioned medium (CM) has anti-inflammatory and immunomodulatory effects. The aim of the present study was to investigate whether hAMSC-CM exerts protective effects against PQ toxicity in A549 cells. The data demonstrated that the activity of A549 cells was decreased after 24 h of PQ exposure and that the cell viability of the hAMSC-CM intervention group was higher compared with the PQ-only group. hAMSC-CM intervention decreased cell damage, apoptosis rates, oxidative stress indexes, Bax/Bcl-2 ratios and CHOP expression levels in poisoned cells by CCK-8 experiment, apoptosis detection, ROS content detection, and Western blot analysis respectively. In conclusion, hAMSC-CM may attenuate the cell damage caused by PQ by reducing endoplasmic reticulum stress and oxidative stress.