bluuebunny/crossref_metadata_2025_split huggingface dataset:
Blood copper and risk of cardiometabolic diseases: a Mendelian randomization study
Authors: Jäger Susanne , Cabral Maria , Kopp Johannes F , Hoffmann Per , Ng Esther , Whitfield John B , Morris Andrew P , Lind Lars , Schwerdtle Tanja , Schulze Matthias B 
Published: September 2021

Abstract
Observational evidence links higher blood levels of copper with higher risk of cardiovascular diseases. However, whether those associations reflect causal links or can be attributed to confounding is still not fully clear. We investigated causal effects of copper on the risk of cardiometabolic endpoints (stroke, coronary artery disease [CAD] and type 2 diabetes) and cardiometabolic risk factors in two-sample Mendelian randomization (MR) studies. The selection of genetic instruments for blood copper levels relied on meta-analysis of genome-wide association studies in three independent studies (European Prospective Investigation into Cancer and Nutrition-Potsdam study, Prospective investigation of the Vasculature in Uppsala Seniors study, Queensland Institute of Medical Research studies). For the selected instruments, outcome associations were drawn from large public genetic consortia on the respective disease endpoints (MEGASTROKE, Cardiogram, DIAGRAM) and cardiometabolic risk factors. MR results indicate an inverse association for genetically higher copper levels with risk of CAD (odds ratio [95% confidence interval] = 0.92 [0.86–0.99], P = 0.022) and systolic blood pressure (beta [standard error (SE)] = −0.238 [0.121]; P = 0.049). Multivariable MR incorporating copper and systolic blood pressure into one model suggested systolic blood pressure as mediating factor between copper and CAD risk. In contrast to previous observational evidence establishing higher blood copper levels as risk-increasing factor for cardiometabolic diseases, this study suggests that higher levels of genetically predicted copper might play a protective role for the development of CAD and systolic blood pressure.
Genetic incompatibility of the reproductive partners: an evolutionary perspective on infertility
Authors: Kekäläinen Jukka 
Published: September 2021

ABSTRACT
In natural fertilisation, the female reproductive tract allows only a strictly selected sperm subpopulation to proceed in the vicinity of an unfertilised oocyte. Female-mediated sperm selection (also known as cryptic female choice (CFC)) is far from a random process, which frequently biases paternity towards particular males over others. Earlier studies have shown that CFC is a ubiquitous phenomenon in the animal kingdom and often promotes assortative fertilisation between genetically compatible mates. Here, I demonstrate that CFC for genetic compatibility likely also occurs in humans and is mediated by a complex network of interacting male and female genes. I also show that the relative contribution of genetic compatibility (i.e. the male–female interaction effect) to reproductive success is generally high and frequently outweighs the effects of individual males and females. Together, these facts indicate that, along with male- and female-dependent pathological factors, reproductive failure can also result from gamete-level incompatibility of the reproductive partners. Therefore, I argue that a deeper understanding of these evolutionary mechanisms of sperm selection can pave the way towards a more inclusive view of infertility and open novel possibilities for the development of more personalised infertility diagnostics and treatments.
The microbial contribution to the trophic position of stomiiform fishes
Authors: Bode Antonio , Olivar M Pilar , López-Pérez Cristina , Hernández-León Santiago 
Published: September 2021

Abstract
The trophic position (TP) of fishes determines their importance in terms of energy flows within food webs. However, accurate estimations of TP are often prevented because of the difficulties in tracing all food sources. This is particularly challenging for omnivorous fishes, such as those from the Order Stomiiformes. In this study, we applied recent developments in stable isotope analysis of amino acids to untangle the contributions of microbial vs. metazoan food webs in 13 species of Stomiiformes. The inclusion of the microbial food web reduced the differences between TP estimates using stable isotopes and those derived from stomach content analysis. In addition, the new estimates allowed to quantify the relative contribution of the microbial food web to each species (6–21%), highlighting the importance of detritus consumption even in piscivorous species (e.g. Stomias boa and Chauliodus danae). The comparison of TP estimates obtained with selected amino acids in fish muscle allowed for the detection of the microbial influence integrated at time scales relevant for net fish growth, even when trophic exchanges in the microbial food web occur at much shorter time scales. The assessment of TP considering the differential contribution of microbial and metazoan food webs challenges our current understanding of marine food webs; yet provides a new quantitative tool for the analysis of their structure and function.
Life-Course Approach to Vaccination in Bangladesh for Meeting the Health and Health-Related Sustainable Development Goals: A Commentary
Authors: Bhuiyan Taufiqur Rahman , Islam Taufiqul , Qadri Firdausi 
Published: September 2021

Abstract
Bangladesh is entering from low-income to lower-middle-income status in 2020, and this will be completed in the next 5 years. With gross national income growing, vaccines will need to be procured through private market for the Expanded Program on Immunization. A cost-benefit analysis is needed to evaluate vaccine demand in different socioeconomic groups in the country, to inform this procurement. Moreover, disease burden studies and awareness of importance of specific vaccines are needed as we move forward. A life-course approach to vaccination may enable whole society to realize the full potential of vaccination and address most significant threats to its success over time.
Impact of PrsA on membrane lipid composition during daptomycin-resistance-mediated β-lactam sensitization in clinical MRSA strains
Authors: de Carvalho Carla C C R , Taglialegna Agustina , Rosato Adriana E 
Published: September 2021

Abstract

Background
The cyclic anionic lipopeptide daptomycin is used in the treatment of severe infections caused by Gram-positive pathogens, including MRSA. Daptomycin resistance, although rare, often results in treatment failure. Paradoxically, in MRSA, daptomycin resistance is usually accompanied by a concomitant decrease in β-lactam resistance in what is known as the ‘see-saw effect’. This resensitization is extensively used for the treatment of MRSA infections, by combining daptomycin and a β-lactam antibiotic, such as oxacillin.

Objectives
We aimed: (i) to investigate the combined effects of daptomycin and oxacillin on the lipid composition of the cellular membrane of both daptomycin-resistant and -susceptible MRSA strains; and (ii) to assess the involvement of the post-translocational protein PrsA, which plays an important role in oxacillin resistance in MRSA, in membrane lipid composition and remodelling during daptomycin resistance/β-lactam sensitization.

Results
The combination of microbiological and biochemical studies, with fluorescence microscopy using lipid probes, showed that the lipid composition and surface charge of the daptomycin-resistant cells exposed to daptomycin/oxacillin were dependent on antibiotic concentration and directly associated with PrsA, which influenced cardiolipin remodelling/relocation.

Conclusions
Our findings show that PrsA, in addition to its post-transcriptional role in the maturation of PBP 2a, is a key mediator of cell membrane remodelling connected to the see-saw effect and may have a key role in the resensitization of daptomycin-resistant strains to β-lactams, such as oxacillin.
Comparative Evaluation of the SMARTCHECK® Listeria monocytogenes Detection Kit for GENECHECKER® UF-300 Real-Time PCR System for Select Foods: AOAC Performance Tested MethodSM 042101
Authors: Park Jinhee , Choi Okran , Lee Dobu 
Published: September 2021

Abstract

Background
The candidate method, SMARTCHEK® Listeria monocytogenes Detection Kit, is designed for rapid detection of L. monocytogenes in select food matrixes, for use in conjunction with the Genesystems Rapi:chipTM and GENECHECKER®UF-300 real-time PCR system.

Objective
The independent laboratory evaluation consisted of an inclusivity and exclusivity study, product consistency study, robustness study, stability study, and a matrix study. The paired matrix study tested stainless-steel environmental sponges, uncured ready-to-eat deli turkey, ready-to-eat deli ham, cooked shrimp, and fresh bagged spinach. The robustness study evaluated the method by changing three parameters. The accelerated stability and product consistency study was conducted at 8, 16, 24, 32, and 40 days.

Methods
The candidate method was compared to the US Department of Agriculture Food Safety and Inspection Service Microbiology Laboratory Guidebook, 8.11 Isolation and Identification of Listeria monocytogenes from Red Meat, Poultry, Ready-to-Eat Siluriformes (Fish) and Egg Products, and Environmental Samples and to the US Food and Drug Administration Bacteriological Analytical Manual Chapter 10: Detection of Listeria monocytogenes in foods and Environmental Samples, and Enumeration of Listeria monocytogenes in Foods following a paired study design.

Results
All 50 L. monocytogenes isolates were detected in all three broths tested for inclusivity, while all 37 non-L. monocytogenes isolates were correctly excluded. In the matrix study, the candidate method showed no significant differences between confirmed candidate method results and reference method results for all food matrixes evaluated.

Conclusion
The candidate method is an effective method for the qualitative detection of L. monocytogenes in select food matrixes.

Highlights
The SMARTCHECK Listeria monocytogenes Detection Kit assay allows for fast, reliable detection of L. monocytogenes with results obtained in approximately 35 min after 18–48 h pre-enrichment, depending on the matrix.
β-Hydroxy β-methylbutyrate supplementation to adult Thoroughbred geldings increases type IIA fiber content in the gluteus medius
Authors: Busse Nicolas I , Gonzalez Madison L , Krason Mackenzie L , Johnson Sally E 
Published: September 2021

Abstract
Consumption of β-hydroxy β-methylbutyrate (HMB) alters muscle composition and metabolism leading to strength and agility improvements in human athletes. To determine if HMB affects athletic performance and muscle function in horses, Thoroughbred geldings were fed a control (CON; n = 5) or HMB (n = 6) supplement for 6 wk prior to completing a standardized exercise test (SET). Gluteus medius (GM) muscle biopsies were obtained before the SET for fiber typing. Heart rate, biceps femoris (BF) and semitendinosus (ST) surface electromyograms (EMG), and fore and hind limbs metacarpophalangeal joint angles were captured at the gallop of the SET. Results demonstrate that HMB supplementation increased (P &lt; 0.05) the percentage of type IIA and IIA/X muscle fibers in the GM with a corresponding decrease (P &lt; 0.05) in type IIX fibers. The percentage of type I fibers was unaffected by diet. Supplementation with HMB did not result in any measurable effects on performance or biomechanical properties by comparison to CON. Supplementation with HMB resulted in an increase (P &lt; 0.05) in ST median frequency at speeds of 10 m/s and greater. Increasing treadmill speed resulted in an increase (P &lt; 0.05) in stride length and the maximal proximal forelimb fetlock angle, and a decrease (P &lt; 0.05) in stance phase time of the gait cycle. Integrated EMG (iEMG) increased (P &lt; 0.05) with increasing treadmill speeds for both the BF and ST with the BF exhibiting greater (P &lt; 0.05) iEMG values than the ST. In summary, HMB increased the percentage of type IIA GM fibers, which did not translate into improved performance.
Promazine Detection in Human Hair: Presentation of an LC–MS-MS Method and Pattern of Drug Discontinuation
Authors: Kintz Pascal , Ameline Alice , Raul Jean-Sébastien 
Published: September 2021

Abstract
Promazine is one of the oldest phenothiazine derivatives that have been proposed for the treatment of various psychiatric disorders. The drug is available as tablets, as syrups and in injectable forms. Despite its prescription to millions of subjects, its detection in human hair has seldom been reported. The aim of the present work is to develop a specific method to identify promazine in human hair by liquid chromatography–tandem mass spectrometry and to apply it to a patient who was self-medicating. The method involves overnight incubation of 20 mg of cut hair in 1 mL of pH 9.5 borate buffer in the presence of amitriptyline-d3 at 40°C. The chromatographic separation was performed using a reverse phase column HSS C18 with a gradient elution for 15 min. Linearity was verified from 0.5 to 500 pg/mg (r2 = 0.9996), after spiking blank hair with the corresponding amounts of promazine. The limit of detection was estimated at 0.1 pg/mg. The precision was lower than 20%. Promazine was detected in the hair of a psychotic subject at 228–270 pg/mg in a 3 × 1 cm segment. Given this was a patient who was self-medicating, her physician requested an immediate drug discontinuation. In a fresh hair specimen collected 3 months later, the proximal segment (0–1 cm) tested positive at 0.9 pg/mg, clearly indicating that the time to obtain a negative result after promazine discontinuation is about 3–4 months.
Evaluation of quality indicators near death in older adult cancer decedents in Japan: A nationwide retrospective cohort study
Authors: Shirane Sachie , Michihata Nobuaki , Yoshiuchi Kazuhiro , Ariyoshi Keisuke , Iwase Satoru , Morita Kojiro , Matsui Hiroki , Fushimi Kiyohide , Yasunaga Hideo 
Published: September 2021

Abstract

Objectives
End-of-life cancer care is important; however, data on hospitalization and costs for older patients have been lacking. We aimed to examine quality indicators and costs for older patients in Japan.

Methods
Using the Diagnosis Procedure Combination database, a national database of acute-care hospitals in Japan, we retrospectively collected data on cancer decedents aged ≥65 years. We evaluated the quality indicators (hospitalizations, length of stay in the hospital, emergency hospitalizations, emergency hospitalizations using an ambulance, intensive care unit [ICU] admissions, length of stay in the ICU, interval between last chemotherapy use and death, and chemotherapy within 14 days before death) and hospitalization costs at 30, 90 and 180 days before death. We compared the outcomes across age groups (65–74, 75–84 and ≥ 85 years).

Results
Between January 2011 and March 2015, we identified 369 616 cancer decedents. From 180 to 30 days before death, there were increases in emergency hospitalizations, emergency hospitalizations using an ambulance, and the mean costs per hospital day. Overall, 16.7% of patients receiving chemotherapy last received this treatment on the day before death or the day of death. Costs decreased with increasing age. The group aged ≥85 years had the shortest hospital and ICU stays and the lowest multiple hospitalizations, ICU admissions, chemotherapy within 14 days before death, and costs.

Conclusions
Many older adult patients had emergency hospitalizations and received chemotherapy just prior to death, and there is room for improvement in appropriate end-of-life care. Oldest old patients consumed relatively few medical resources.
Meta-Analysis of Collaboration and Performance: Moderating Tests of Sectoral Differences in Collaborative Performance
Authors: Lee David , Hung ChiaKo 
Published: September 2021

AbstractOver the past few decades, collaboration has flourished in the public administration and policy fields as a rational means to solve complex issues and improve public service performance. Through a meta-analysis of 26 studies with 251 effect sizes, this investigation provides novel perspectives for understanding the effects of different collaborative partnerships on performance. To test these mechanisms, we applied various social science theories, such as institutional theory, resource dependence theory, a resource-based view, and transaction cost theories. Our findings indicate that the overall effect of collaborative performance is positive and significant. Moreover, meta-regression results show that public–public collaboration results in better performance than public–nonprofit or public–business collaboration, while involving all three entity types in collaborative efforts yields similar outcomes to public–public collaboration. Several implications of these findings are outlined for researchers and practitioners.
Translational Aspects of the Immunology of Clostridioides difficile Infection: Implications for Pediatric Populations
Authors: Kociolek Larry K , Zackular Joseph P , Savidge Tor 
Published: September 2021

Abstract
Clostridioides difficile has become the most common healthcare-associated pathogen in the United States, leading the US Centers for Disease Control and Prevention (CDC) to classify C. difficile as an “urgent” public health threat that requires “urgent and aggressive action.” This call to action has led to new discoveries that have advanced our understanding of Clostridioides difficile infection (CDI) immunology and clinical development of immunologic-based therapies for CDI prevention. However, CDI immunology research has been limited in pediatric populations, and several unanswered questions remain regarding the function of host immune response in pediatric CDI pathogenesis and the potential role of immunologic-based therapies in children. This review summarizes the innate and adaptive immune responses previously characterized in animals and humans and provides a current update on clinical development of immunologic-based therapies for CDI prevention in adults and children. These data inform the future research needs for children.
Oxymatrine screened from Sophora flavescens by cell membrane immobilized chromatography relieves histamine-independent itch
Authors: Zhang Zhe , Pan Jianhao , Zhu Tao , Malewicz Nathalie , Ye Kaihe , Rong Jianhui , Luo Yong , Situ Yongli , Verkhratsky Alexei , Wang Yong , Zhao Jianhao , Tang Dan , Nie Hong 
Published: September 2021

Abstract

Objectives
This study aimed to discover the active compounds of Sophora flavescens Ait. (SF), the anti-itch effects and underlying mechanisms of oxymatrine (OMT), one of the bioactive compounds from SF.

Methods
Dorsal root ganglion cell membrane immobilized chromatography was used to screen potential anti-pruritic active compounds from SF. The scratching behaviour was analysed to systematically study the anti-pruritic effects of OMT in chloroquine- (CQ), peptide Ser–Leu–Ile–Gly–Arg–Leu- (SLIGRL), histamine- (HIS) and allyl-isothiocyanate-(AITC)-induced itch mice models. Real-time quantitative PCR, in-vivo study and molecular docking were employed to explore the underlying mechanisms.

Key findings
All in all, 21 compounds of SF were identified and 5 potential bioactive compounds were discovered. OMT significantly reduced scratching bouts in two HIS-independent itch models induced by CQ and SLIGRL but was not effective in the HIS-induced itch model. OMT reduced scratching bouts in a dose-dependent manner and decreased the messenger RNA (mRNA) expression of transient receptor potential ankyrin 1 (TRPA1) channel in two HIS-independent itch models; in addition, OMT reduced the wipes and scratching bouts induced by AITC.

Conclusions
This study discovered five potential anti-pruritic compounds including OMT in the SF extract, and OMT has strong anti-pruritic effects in HIS-independent itch via TRPA1 channel.
Point spread function reconstruction of adaptive-optics imaging: meeting the astrometric requirements for time-delay cosmography
Authors: Chen Geoff C-F , Treu Tommaso , Fassnacht Christopher D , Ragland Sam , Schmidt Thomas , Suyu Sherry H 
Published: September 2021

ABSTRACT
Astrometric precision and knowledge of the point spread function are key ingredients for a wide range of astrophysical studies including time-delay cosmography in which strongly lensed quasar systems are used to determine the Hubble constant and other cosmological parameters. Astrometric uncertainty on the positions of the multiply-imaged point sources contributes to the overall uncertainty in inferred distances and therefore the Hubble constant. Similarly, knowledge of the wings of the point spread function is necessary to disentangle light from the background sources and the foreground deflector. We analyse adaptive optics (AO) images of the strong lens system J 0659+1629 obtained with the W. M. Keck Observatory using the laser guide star AO system. We show that by using a reconstructed point spread function we can (i) obtain astrometric precision of &lt;1 mas, which is more than sufficient for time-delay cosmography; and (ii) subtract all point-like images resulting in residuals consistent with the noise level. The method we have developed is not limited to strong lensing, and is generally applicable to a wide range of scientific cases that have multiple point sources nearby.
The dispersal of protoplanetary discs – II: photoevaporation models with observationally derived irradiating spectra
Authors: Ercolano Barbara , Picogna Giovanni , Monsch Kristina , Drake Jeremy J , Preibisch Thomas 
Published: September 2021

ABSTRACT
Young solar-type stars are known to be strong X-ray emitters and their X-ray spectra have been widely studied. X-rays from the central star may play a crucial role in the thermodynamics and chemistry of the circumstellar material as well as in the atmospheric evolution of young planets. In this paper, we present model spectra based on spectral parameters derived from the observations of young stars in the Orion nebula cluster from the Chandra Orion Ultradeep Project (COUP). The spectra are then used to calculate new photoevaporation prescriptions that can be used in disc and planet population synthesis models. Our models clearly show that disc wind mass loss rates are controlled by the stellar luminosity in the soft ($100\, \mathrm{eV}$ to $1\, \mathrm{keV}$) X-ray band. New analytical relations are provided for the mass loss rates and profiles of photoevaporative winds as a function of the luminosity in the soft X-ray band. The agreement between observed and predicted transition disc statistics moderately improved using the new spectra, but the observed population of strongly accreting large cavity discs can still not be reproduced by these models. Furthermore, our models predict a population of non-accreting transition discs that are not observed. This highlights the importance of considering the depletion of millimetre-sized dust grains from the outer disc, which is a likely reason why such discs have not been detected yet.