bluuebunny/crossref_metadata_2025_split huggingface dataset:
Contributions of asthma, rhinitis and IgE to exhaled nitric oxide in adolescents
Authors: Flashner Bess M. , Rifas-Shiman Sheryl L. , Oken Emily , Camargo Carlos A. , Platts-Mills Thomas A.E. , Workman Lisa , Litonjua Augusto A. , Gold Diane R. , Rice Mary B. 
Published: April 2021

Exhaled nitric oxide fraction (FeNO) is an indicator of allergic airway inflammation. However, it is unknown how asthma, allergic rhinitis (AR) and allergic sensitisation relate toFeNO, particularly among adolescents and in overlapping conditions. We sought to determine the associations between asthma, AR, and aeroallergen immunoglobulin (Ig)E andFeNOin adolescents.We measuredFeNOamong 929 adolescents (aged 11–16 years) in Project Viva, an unselected prebirth cohort in Massachusetts, USA. We defined asthma as ever asthma physician diagnosis plus wheezing in the past year or taking asthma medications in the past month, AR as a physician diagnosis of hay fever or AR, and aeroallergen IgE as any IgE >0.35 IU·mL−1among 592 participants who provided blood samples. We examined associations of asthma, AR and IgE with percent difference inFeNOin linear regression models adjusted for sex, race/ethnicity, age and height, maternal education and smoking during pregnancy, and household/neighbourhood demographics.Asthma (14%) was associated with 97% higherFeNO(95% CI 70–128%), AR (21%) with 45% higherFeNO(95% CI 28–65%), and aeroallergen IgE (58%) with 102% higherFeNO(95% CI 80–126%) compared to those without each condition, respectively. In the absence of asthma or AR, aeroallergen IgE was associated with 75% higherFeNO(95% CI 52–101), while asthma and AR were not associated withFeNOin the absence of IgE.The link between asthma and AR withFeNOis limited to those with IgE-mediated phenotypes.FeNOmay be elevated in those with allergic sensitisation alone, even in the absence of asthma or AR.
Incorporating foot assessment in the comprehensive geriatric assessment
Authors: Iseli Rebecca K. , Duncan Gregory , Lee Elton K. , Lewis Ellen , Maier Andrea B. 
Published: April 2021

Abstract
Background
Foot problems are common in older adults and associated with poorer physical function, falls, frailty and reduced quality of life. Comprehensive Geriatric Assessment (CGA), a multidisciplinary process that is considered the gold standard of care for older adults, does not routinely include podiatry assessment and intervention in hospitalized older adults.

Aims
To introduce foot assessment to inpatient CGA to determine prevalence of foot disease, foot disease risk factors and inappropriate footwear use, assess inter-rater reliability of foot assessments, determine current podiatry input and examine associations between patient characteristics and foot disease risks.

Methods
Prospective, observational cohort study of older adults on geriatric rehabilitation wards. Foot assessment completed using the Queensland Foot Disease Form (QFDF) in addition to routine CGA.

Results
Fifty-two patients (median age [inter-quartile range] 86.4 [79.2–90.3] years, 54% female) were included. Six patients (12%) had foot disease and 13 (25%) had a ‘high risk’ or ‘at risk’ foot. Foot disease risk factor prevalence was peripheral arterial disease 9 (17%); neuropathy 10 (19%) and foot deformity 11 (22%). Forty-one patients (85%) wore inappropriate footwear. Inter-rater agreement was substantial on presence of foot disease and arterial disease, fair to moderate on foot deformity and fair on neuropathy and inappropriate footwear. Eight patients (15%) saw a podiatrist during admission: 5 with foot disease, 1 ‘at risk’ and 2 ‘low risk’ for foot disease. Patients with an at risk foot or foot disease had significantly longer median length of hospital stay (25 [13.7–32.1] vs 15.2 [8–22.1] days, p = 0.01) and higher median Malnutrition Screening Test scores (2 [0–3] vs 0 [0–2], p = 0.03) than the low-risk group. Patients with foot disease were most likely to see a podiatrist (p < 0.001).

Conclusion
Foot disease, foot disease risk factors and inappropriate footwear are common in hospitalized older adults, however podiatry assessment and intervention is mostly limited to patients with foot disease. Addition of routine podiatry assessment to the multidisciplinary CGA team should be considered. Examination for arterial disease and risk of malnutrition may be useful to identify at risk patients for podiatry review.
“Randomized trial of physical activity on quality of life and lung cancer biomarkers in patients with advanced stage lung cancer: a pilot study”
Authors: Bade Brett C. , Gan Geliang , Li Fangyong , Lu Lingeng , Tanoue Lynn , Silvestri Gerard A. , Irwin Melinda L. 
Published: April 2021

Abstract
Background
Lung cancer survivors need more options to improve quality of life (QoL). It is unclear to what extent patients with advanced stage disease are willing to participate in home-based physical activity (PA) and if these interventions improve QoL. The goal of our study was to determine interest in participating in our 3-month home-based walking regimen in patients with advanced stage lung cancer. We used a randomized design to evaluate for potential benefit in PA and patient-reported outcomes.

Methods
We performed an open-label, 1:1 randomized trial in 40 patients with stage III/IV non-small cell lung cancer (NSCLC) evaluating enrollment rate, PA, QoL, dyspnea, depression, and biomarkers. Compared to usual care (UC), the intervention group (IG) received an accelerometer, in-person teaching session, and gain-framed text messages for 12 weeks.

Results
We enrolled 56% (40/71) of eligible patients. Participants were on average 65 years and enrolled 1.9 years from diagnosis. Most patients were women (75%), and receiving treatment (85%) for stage IV (73%) adenocarcinoma (83%). A minority of patients were employed part-time or full time (38%). Both groups reported low baseline PA (IG mean 37 (Standard deviation (SD) 46) vs UC 59 (SD 56) minutes/week; p = 0.25). The IG increased PA more than UC (mean change IG + 123 (SD 212) vs UC + 35 (SD 103) minutes/week; p = 0.051)). Step count in the IG was not statistically different between baseline (4707 step/day), week 6 (5605; p = 0.16), and week 12 (4606 steps/day; p = 0.87). The intervention improved EORTC role functioning domain (17 points; p = 0.022) with borderline improvement in dyspnea (− 13 points; p = 0.051) compared to UC. In patients with two blood samples (25%), we observed a significant increase in soluble PD-1 (219.8 (SD 54.5) pg/mL; p < 0.001).

Conclusions
Our pilot trial using a 3-month, home-based, mobile health intervention enrolled over half of eligible patients with stage III and IV NSCLC. The intervention increased PA, and may improve several aspects of QoL. We also identified potential biomarker changes relevant to lung cancer biology. Future research should use a larger sample to examine the effect of exercise on cancer biomarkers, which may mediate the association between PA and QoL.

Clinical trial registration
Clinicaltrials.gov (NCT03352245).
Apixaban anti-Xa level monitoring in treatment of acute upper extremity deep vein thrombosis for patient on chronic hemodialysis: a case report
Authors: Roberge Guillaume , Wells Philip Stephen 
Published: April 2021

Abstract
Background
Patients on dialysis are at higher risk of major bleeding and recurrent thrombosis creating acute venous thromboembolism (VTE) treatment challenges. DOACs represent an interesting option but there are concerns of bioaccumulation and increased bleeding risk. Anti-Xa trough levels may be used to monitor for bioaccumulation but there is little data.

Case presentation
We describe a case, a 51 yo female, 36 kg on hemodialysis with a provoked acute upper extremity deep vein thrombosis in whom body habitus and calciphylaxis contraindicated the use of standard therapy. She received apixaban 2.5 mg twice daily for 6 weeks. The apixaban anti-Xa trough levels were measured weekly 12 h after the morning dose and ranged from 58 to 84 ng/mL, similar to expected levels with normal renal function. There were no adverse events in the 3 months follow-up.

Conclusions
We saw no evidence of bioaccumulation indicating a potential role for low dose apixaban for acute VTE in dialysis patients.
Tribbles homolog 2 (Trib2), a pseudo serine/threonine kinase in tumorigenesis and stem cell fate decisions
Authors: Fang Yu , Zekiy Angelina Olegovna , Ghaedrahmati Farhoodeh , Timoshin Anton , Farzaneh Maryam , Anbiyaiee Amir , Khoshnam Seyed Esmaeil 
Published: April 2021

AbstractThe family of Tribbles proteins play many critical nonenzymatic roles and regulate a wide range of key signaling pathways. Tribbles homolog 2 (Trib2) is a pseudo serine/threonine kinase that functions as a scaffold or adaptor in various physiological and pathological processes. Trib2 can interact with E3 ubiquitin ligases and control protein stability of downstream effectors. This protein is induced by mitogens and enhances the propagation of several cancer cells, including myeloid leukemia, liver, lung, skin, bone, brain, and pancreatic. Thus, Trib2 can be a predictive and valuable biomarker for the diagnosis and treatment of cancer. Recent studies have illustrated that Trib2 plays a major role in cell fate determination of stem cells. Stem cells have the capacity to self-renew and differentiate into specific cell types. Stem cells are important sources for cell-based regenerative medicine and drug screening. Trib2 has been found to increase the self-renewal ability of embryonic stem cells, the reprogramming efficiency of somatic cells, and chondrogenesis. In this review, we will focus on the recent advances of Trib2 function in tumorigenesis and stem cell fate decisions.
Diagnosis and treatment of type 1 diabetes at the dawn of the personalized medicine era
Authors: Akil Ammira Al-Shabeeb , Yassin Esraa , Al-Maraghi Aljazi , Aliyev Elbay , Al-Malki Khulod , Fakhro Khalid A. 
Published: April 2021

AbstractType 1 diabetes affects millions of people globally and requires careful management to avoid serious long-term complications, including heart and kidney disease, stroke, and loss of sight. The type 1 diabetes patient cohort is highly heterogeneous, with individuals presenting with disease at different stages and severities, arising from distinct etiologies, and overlaying varied genetic backgrounds. At present, the “one-size-fits-all” treatment for type 1 diabetes is exogenic insulin substitution therapy, but this approach fails to achieve optimal blood glucose control in many individuals. With advances in our understanding of early-stage diabetes development, diabetes stratification, and the role of genetics, type 1 diabetes is a promising candidate for a personalized medicine approach, which aims to apply “the right therapy at the right time, to the right patient”. In the case of type 1 diabetes, great efforts are now being focused on risk stratification for diabetes development to enable pre-clinical detection, and the application of treatments such as gene therapy, to prevent pancreatic destruction in a sub-set of patients. Alongside this, breakthroughs in stem cell therapies hold great promise for the regeneration of pancreatic tissues in some individuals. Here we review the recent initiatives in the field of personalized medicine for type 1 diabetes, including the latest discoveries in stem cell and gene therapy for the disease, and current obstacles that must be overcome before the dream of personalized medicine for all type 1 diabetes patients can be realized.
HBP1-mediated transcriptional repression of AFP inhibits hepatoma progression
Authors: Cao Zhengyi , Cheng Yuning , Wang Jiyin , Liu Yujuan , Yang Ruixiang , Jiang Wei , Li Hui , Zhang Xiaowei 
Published: April 2021

Abstract
Background
Hepatoma is a common malignancy of the liver. The abnormal high expression of alpha-fetoprotein (AFP) is intimately associated with hepatoma progress, but the mechanism of transcriptional regulation and singularly activation of AFP gene in hepatoma is not clear.

Methods
The expression of transcription factor HBP1 and AFP and clinical significance were further analyzed in hepatoma tissues from the patients who received surgery or TACE and then monitored for relapse for up 10 years. HBP1-mediated transcriptional regulation of AFP was analyzed by Western blotting, Luciferase assay, Realtime-PCR, ChIP and EMSA. After verified the axis of HBP-AFP, its impact on hepatoma was measured by MTT, Transwell and FACS in hepatoma cells and by tumorigenesis in HBP1−/− mice.

Results
The relative expressions of HBP1 and AFP correlated with survival and prognosis in hepatoma patients. HBP1 repressed the expression of AFP gene by directly binding to the AFP gene promoter. Hepatitis B Virus (HBV)-encoded protein HBx promoted malignancy in hepatoma cells through binding to HBP1 directly. Icaritin, an active ingredient of Chinese herb epimedium, inhibited malignancy in hepatoma cells through enhancing HBP1 transrepression of AFP. The repression of AFP by HBP1 attenuated AFP effect on PTEN, MMP9 and caspase-3, thus inhibited proliferation and migration, and induced apoptosis in hepatoma cells. The deregulation of AFP by HBP1 contributed to hepatoma progression in mice.

Conclusions
Our data clarify the mechanism of HBP1 in inhibiting the expression of AFP and its suppression in malignancy of hepatoma cells, providing a more comprehensive theoretical basis and potential solutions for the diagnosis and treatment of hepatoma.
Should the BCRA1/2-mutations healthy carriers be valid candidates for hematopoietic stem cell donation?
Authors: Fresa Alberto , Sica Simona 
Published: April 2021

AbstractIt’s still not clear whether the mutational status of BRCA-mutated healthy hematopoietic stem cells (HSCs) donors could have an impact on the engraftment. Comparing the studies present in literature, we focused on the correlation between BRCA mutations and the development of hematological malignancies and Fanconi anemia (FA); then, we explored HSCs types, frequencies, and functions in the presence of BRCA mutations, as well as the reconstitution of hematopoiesis after chemotherapy and radiation treatments. The role of BRCA mutations in the FA showed a possible involvement in the onset of the disease; the mutation carriers, indeed, did not show any sign of the typical phenotype of the FA. BRCA mutational status can be considered as a risk factor for hematological malignancies, but only for secondary malignancies and/or in the presence of bone marrow stress factors. Currently we don’t know if a conditioning regimen could be compensated by BRCA mutated HSCs, even if murine models tried to show the possible differences between fully mutated, haploinsufficient and normal HSCs. Thus, given the downregulating effect of the mutations on hematopoiesis, it could be questionable to use the HSCs of a BRCA-mutated donor in the presence of another available donor with the same compatibility.
Antibacterial activity of royal jelly-mediated green synthesized silver nanoparticles
Authors: Gevorgyan Susanna , Schubert Robin , Yeranosyan Mkrtich , Gabrielyan Lilit , Trchounian Armen , Lorenzen Kristina , Trchounian Karen 
Published: April 2021

AbstractThe application of green synthesis in nanotechnology is growing day by day. It’s a safe and eco-friendly alternative to conventional methods. The current research aimed to study raw royal jelly’s potential in the green synthesis of silver nanoparticles and their antibacterial activity. Royal jelly served as a reducing and oxidizing agent in the green synthesis technology of colloidal silver nanoparticles. The UV–Vis maximum absorption at ~ 430 nm and fluorescence emission peaks at ~ 487 nm confirmed the presence of Ag NPs. Morphology and structural properties of Ag NPs and the effect of ultrasound studies revealed: (i) the formation of polydispersed and spherical particles with different sizes; (ii) size reduction and homogeneity increase by ultrasound treatment. Antibacterial activity of different concentrations of green synthesized Ag NPs has been assessed on Gram-negative S. typhimurium and Gram-positive S. aureus, revealing higher sensitivity on Gram-negative bacteria.
How property rights influence equity, efficiency and sustainability of high-altitude rangeland management in Bhutan
Authors: Tenzing Karma , Millar Joanne , Black Rosemary 
Published: April 2021

AbstractThis paper explores how property rights arrangements affect the equity, efficiency and sustainability of high-altitude rangeland management in Bhutan from the perspectives of rangeland users. Property rights affect how natural resources are allocated to individuals, communities and institutions and how they are managed. In Bhutan, only grazing rights are granted to rangeland users, as ownership of rangeland lies with the State. Three case study areas were selected across Bhutan with different levels of rangeland degradation, governance arrangements and property rights regimes. Semi-nomadic yak herders, sedentary livestock farmers and government officials participated in 40 semi-structured interviews and nine focus group discussions between 2013 and 2014. Findings revealed that historical inequities exist with private leases and some communal leases, but government-supported leases with management rights are considered more equitable. All property rights systems can become unproductive and inefficient with increasing populations, but communal areas are at greater risk of sub-optimal resource use and conflicts. Respondents were concerned about the lack of management rights, uncertainty over future lease arrangements and climate change impacts. This research revealed that (i) assignment of incomplete property rights is detrimental to efficient and sustainable natural resource management; (ii) banning of traditional practices such as burning, clearing and cutting of shrubs and bushes could undermine conservation by triggering natural resource degradation; (iii) granting of management rights in the bundle of rights is vital to encourage provisioning and maintenance activities; and (iv) in the absence of official contracts, deeds or titles which provide tenure security and political legitimacy, equitable distribution, clear boundary demarcation with written norms and rules are not sufficient to foster long-term investment in provisioning and maintenance activities. We discuss the implications of the findings for delayed implementation of the Land Act 2007 aimed at a more equitable redistribution of use rights and improved high-altitude rangeland management in Bhutan.
New approach to calculating tree height at the regional scale
Authors: Li Congrong , Song Jinling , Wang Jindi 
Published: April 2021

Abstract
Background
Determining the spatial distribution of tree heights at the regional area scale is significant when performing forest above-ground biomass estimates in forest resource management research. The geometric-optical mutual shadowing (GOMS) model can be used to invert the forest canopy structural parameters at the regional scale. However, this method can obtain only the ratios among the horizontal canopy diameter (CD), tree height, clear height, and vertical CD. In this paper, we used a semi-variance model to calculate the CD using high spatial resolution images and expanded this method to the regional scale. We then combined the CD results with the forest canopy structural parameter inversion results from the GOMS model to calculate tree heights at the regional scale.

Results
The semi-variance model can be used to calculate the CD at the regional scale that closely matches (mainly with in a range from − 1 to 1 m) the CD derived from the canopy height model (CHM) data. The difference between tree heights calculated by the GOMS model and the tree heights derived from the CHM data was small, with a root mean square error (RMSE) of 1.96 for a 500-m area with high fractional vegetation cover (FVC) (i.e., forest area coverage index values greater than 0.8). Both the inaccuracy of the tree height derived from the CHM data and the unmatched spatial resolution of different datasets will influence the accuracy of the inverted tree height. And the error caused by the unmatched spatial resolution is small in dense forest.

Conclusions
The semi-variance model can be used to calculate the CD at the regional scale, together with the canopy structure parameters inverted by the GOMS model, the mean tree height at the regional scale can be obtained. Our study provides a new approach for calculating tree height and provides further directions for the application of the GOMS model.
An ADePT evaluation for incorporating the TIPPS periodontal health intervention into primary care antenatal programmes to enhance infant birth weight in Palestine: a feasibility study
Authors: Abuhaloob Lamis , Helles Nahla , Mossey Peter , Freeman Ruth 
Published: April 2021

Abstract
Background
A feasibility study was conducted to implement the Talk, Instruct, Practice, Plan and Support (TIPPS) intervention for pregnant women to enhance infant birth weight in a conflict area in Low- and Middle-Income Countries (LMIC). The decision tool, A process for Decision-making after Pilot and feasibility Trials (ADePT), examines the methodological factors identified in a feasibility study, that may require modification for a full trial. Thus, this study aimed to use the ADePT decision tool to evaluate if the feasibility study had achieved its objectives and to identify the need for intervention, clinical context and trial design modification.

Methods
A one-arm, pretest–posttest feasibility study recruited 25 pregnant women in their first trimester and clinic staff from a primary healthcare clinic located in Gaza City, Palestine. The TIPPS periodontal health intervention was delivered by antenatal care nurses to the pregnant women during their regular follow-up appointments. The ADePT framework was applied to evaluate the findings from the feasibility study. The ADePT checklist demonstrated sample size estimation, recruitment, consent, intervention adherence, intervention acceptability, costs and duration, completion and appropriateness of outcome assessments, retention, logistics, and synergy between protocol components.

Results
All recruited pregnant women (25, aged 16–35 years old) consented to participate in the study, and the adherence to the intervention was 88% (22 women). The TIPPS intervention was acceptable, but there was ambivalence over who should deliver it in the clinic. Only the cost of toothbrushing and TIPPS information materials was calculated, while the cost of nurses’ time was not included. The missing values of data were few (12% of gingival bleeding data and 22% from infant birth weight data). This intervention significantly reduced the mean percentage of plaque and bleeding scores after 3 months. The sample size for future randomised controlled trial was estimated around 400 participants. The participants stated the value of the intervention. The clinic staff voiced concerns regarding time and the cost of nurses providing the TIPPS intervention. This allowed suggestions to be made regarding the modification of trial design and context of implementation.

Conclusions
The ADePT evaluation showed it was possible to progress to full trial with modifications in the trial design.