bluuebunny/crossref_metadata_2025_split huggingface dataset:
SARS-CoV-2 neutralizing antibodies in patients with varying severity of acute COVID-19 illness
Authors: Jeewandara Chandima , Jayathilaka Deshni , Gomes Laksiri , Wijewickrama Ananda , Narangoda Eranga , Idampitiya Damayanthi , Guruge Dinuka , Wijayamuni Ruwan , Manilgama Suranga , Ogg Graham S. , Tan Chee Wah , Wang Lin-Fa , Malavige Gathsaurie Neelika 
Published: January 2021

AbstractIn order to support vaccine development, and to aid convalescent plasma therapy, it would be important to understand the kinetics, timing and persistence of SARS-CoV-2 neutralizing antibodies (NAbs), and their association with clinical disease severity. Therefore, we used a surrogate viral neutralization test to evaluate their levels in patients with varying severity of illness, in those with prolonged shedding and those with mild/asymptomatic illness at various time points. Patients with severe or moderate COVID-19 illness had earlier appearance of NAbs at higher levels compared to those with mild or asymptomatic illness. Furthermore, those who had prolonged shedding of the virus, had NAbs appearing faster and at higher levels than those who cleared the virus earlier. During the first week of illness the NAb levels of those with mild illness was significantly less (p = 0.01), compared to those with moderate and severe illness. At the end of 4 weeks (28 days), although 89% had NAbs, 38/76 (50%) in those with > 90 days had a negative result for the presence of NAbs. The Ab levels significantly declined during convalescence (> 90 days since onset of illness), compared to 4 to 8 weeks since onset of illness. Our data show that high levels of NAbs during early illness associated with clinical disease severity and that these antibodies declined in 50% of individuals after 3 months since onset of illness.
High-throughput microbioreactor provides a capable tool for early stage bioprocess development
Authors: Fink Mathias , Cserjan-Puschmann Monika , Reinisch Daniela , Striedner Gerald 
Published: January 2021

AbstractTremendous advancements in cell and protein engineering methodologies and bioinformatics have led to a vast increase in bacterial production clones and recombinant protein variants to be screened and evaluated. Consequently, an urgent need exists for efficient high-throughput (HTP) screening approaches to improve the efficiency in early process development as a basis to speed-up all subsequent steps in the course of process design and engineering. In this study, we selected the BioLector micro-bioreactor (µ-bioreactor) system as an HTP cultivation platform to screen E. coli expression clones producing representative protein candidates for biopharmaceutical applications. We evaluated the extent to which generated clones and condition screening results were transferable and comparable to results from fully controlled bioreactor systems operated in fed-batch mode at moderate or high cell densities. Direct comparison of 22 different production clones showed great transferability. We observed the same growth and expression characteristics, and identical clone rankings except one host-Fab-leader combination. This outcome demonstrates the explanatory power of HTP µ-bioreactor data and the suitability of this platform as a screening tool in upstream development of microbial systems. Fast, reliable, and transferable screening data significantly reduce experiments in fully controlled bioreactor systems and accelerate process development at lower cost.
Metachronous advanced neoplasia after submucosal invasive colorectal cancer resection
Authors: Minamide Tatsunori , Ikematsu Hiroaki , Murano Tatsuro , Kadota Tomohiro , Shinmura Kensuke , Yoda Yusuke , Hori Keisuke , Ito Masaaki , Yano Tomonori 
Published: January 2021

AbstractLittle is known about the incidence of metachronous advanced neoplasia (AN) following resection of submucosal invasive colorectal cancer (SM-CRC). Here, we aimed to assess the occurrence of metachronous AN following SM-CRC resection. We retrospectively reviewed consecutive patients who underwent SM-CRC resection at an academic medical center between 2005 and 2013. Among 343 patients, 250 (72.9%) underwent surgical resection or endoscopic resection followed by surgical resection and 93 (27.1%) underwent only endoscopic resection. During a median follow-up period of 61.5 months, the overall incidence of metachronous AN was 7.6%, and the cumulative incidence at 5 years was 6.1%. The cumulative incidence was significantly higher in the endoscopic resection group than in surgical resection group, in patients with colonic disease than in those with rectal disease, and in patients with synchronous AN than in those without. Multivariate analysis revealed that synchronous AN was the only significant risk factor for metachronous AN (HR 4.35; 95% CI 1.88–10.1). These findings imply that depending on synchronous AN, a surveillance protocol following SM-CRC resection can be changed for better detection of metachronous AN.
Clinicopathological characteristics and outcomes of anti-neutrophil cytoplasmic autoantibody-related renal vasculitis with hyperuricemia: a retrospective case-control study
Authors: Wang Ruiqiang , An Dongyue , Wu Yunqi , Ma Pupu , Guo Yuanyuan , Tang Lin 
Published: January 2021

AbstractThe objective of the study was to evaluate the clinicopathological characteristics and investigate the clinical determinants of patient and renal survival in the first 12 months after diagnosis in anti-neutrophil cytoplasmic antibody (ANCA)-associated renal vasculitis (AAV) patients with hyperuricemia. This was a retrospective case-control study in patients with AAV-related renal injury in the First Affiliated Hospital of Zhengzhou University from January 2014 to April 2019. Patients who met the study criteria were divided into two groups: patients without hyperuricemia (n = 92) and patients with hyperuricemia (n = 55). Participants were followed-up for 12 months, and progressing to end-stage renal disease (ESRD) and death was treated as the endpoint event. We found that the level of serum creatinine was an independent risk factor for hyperuricemia, and the level of serum uric acid was an independent risk factors for renal survival and patient survival in ANCA-associated renal vasculitis patients. The crescents formation and the proportion of fibrous crescent likely contributed to severe clinical characteristics and renal pathological changes in ANCA-associated renal vasculitis patients with hyperuricemia. Hyperuricemia has an important influence on the progression of ANCA-associated renal vasculitis. A good control of serum uric acid may improve the prognosis.
PD-L1 overexpression in EBV-positive gastric cancer is caused by unique genomic or epigenomic mechanisms
Authors: Nakano Hiroshi , Saito Motonobu , Nakajima Shotaro , Saito Katsuharu , Nakayama Yuko , Kase Koji , Yamada Leo , Kanke Yasuyuki , Hanayama Hiroyuki , Onozawa Hisashi , Okayama Hirokazu , Fujita Shotaro , Sakamoto Wataru , Saze Zenichiro , Momma Tomoyuki , Mimura Kosaku , Ohki Shinji , Goto Akiteru , Kono Koji 
Published: January 2021

AbstractEpstein-Barr virus-positive gastric cancer [EBV (+) GC] is a distinct GC subtype with unique genetic and epigenetic aberrations. Here, we examined resected GC samples and publicly available microarray data and The Cancer Genome Atlas (TCGA) database to identify the mechanism underlying overexpression of PD-L1 in EBV (+) GC. We found that high levels of PD-L1 overexpression in EBV (+) GC were caused by focal amplification of CD274. By contrast, relatively high expression of PD-L1 in tumor tissue and infiltrating immune cells correlated with CD8 lymphocyte infiltration and IFN-γ expression via IRF3 activation. Since we reported previously that PD-L1 expression is associated both with the presence of CD8 T cells in the tumor microenvironment and with IFN-γ expression in GC, we examined a database to see whether IFN-γ-associated overexpression of PD-L1 plays a significant role in EBV (+) GC. Immunohistochemical staining showed that expression of the IRF3 signature in clinical GC samples was higher in EBV (+) than in EBV (−) cases. The data presented herein reveal a unique dual mechanism underlying PD-L1 overexpression in EBV (+) GC: high focal amplification of CD274 or IFN-γ-mediated signaling via activation of IRF3.
Detailed characterization of plant-based burgers
Authors: De Marchi Massimo , Costa Angela , Pozza Marta , Goi Arianna , Manuelian Carmen L. 
Published: January 2021

AbstractFlexitarians have reduced their meat consumption showing a rising interest in plant-based meat alternatives with ‘meaty’ characteristics, and we are witnessing an unprecedented growth of meat substitutes in the Western market. However, to our knowledge, no information regarding the ‘simulated beef burgers’ nutritional profile compared to similar meat products has been published yet. Here we show that, whilst both plant-based and meat-based burgers have similar protein profile and saturated fat content, the former are richer in minerals and polyunsaturated fatty acids. We found that the most abundant minerals in both categories were Na, K, P, S, Ca, and Mg; being Na and S content similar between groups. Only six amino acids differed between categories, being hydroxyproline exclusively in meat-based burgers. Plant-based burgers revealed fourfold greater content of n-6 than meat-based burgers, and greater short-chain fatty acids proportion. Our results demonstrate how ‘simulated beef’ products may be authenticated based on some specific nutrients and are a good source of minerals. We believe that there is a need to provide complete and unbiased nutritional information on these ‘new’ vegan products so that consumers can adjust their diet to nutritional needs.
The complex case of Macaronichnus trace fossil affecting rock porosity
Authors: Dorador Javier , Rodríguez-Tovar Francisco J. , Miguez-Salas Olmo 
Published: January 2021

AbstractBioturbation is an important factor for reservoir quality due to the modification of host rock petrophysical properties (i.e., porosity, permeability, and connectivity). However, there is no predictable relationship between bioturbation and its effect on rock properties, due to the variability of the involved ichnological features. A detailed ichnological analysis is necessary to determine how bioturbation affects petrophysical properties in a bioturbated reservoir. Traditionally, ichnological features such as density, tiering, size, orientation, architecture, and fill, have been considered. However, other properties have been undervalued as is the case of lining. Here, we present a detailed study on the effects of Macaronichnus burrows, an ichnotaxon usually related to hydrocarbon exploration due to its high concentration in rock notably affecting petrophysical properties. Macaronichnus, a subhorizontal cylindrical burrow, is characterized by a well-defined and developed outer rim surrounding the tube core. Our data indicates a clear zonation in porosity according to burrow structure, with the lowest porosity in the tube core and higher values associated with the surrounded rim. This duality is determined by the tracemaker grain selective feeding activity and the consequent concentrated cementation. The organism concentrates the lighter minerals in the tube core fill during feeding, favoring post-depositional cementation during diagenesis and this results in lower porosity than the host rock. However, heavy minerals, mainly glauconite, are located in the rim, showing higher porosity. Our results support the view that ichnological analyses are essential to determine reservoir quality in bioturbated reservoirs, evidencing that other ichnological properties in addition to those traditionally considered must be evaluated.
Multiparametric ultrasound findings in acute kidney failure due to rare renal cortical necrosis
Authors: Spiesecke Paul , Münch Frédéric , Fischer Thomas , Hamm Bernd , Lerchbaumer Markus H. 
Published: January 2021

AbstractRenal cortical necrosis (RCN) is a rare cause of acute kidney failure and is usually diagnosed on the basis of characteristic enhancement patterns on cross-sectional imaging. Contrast-enhanced ultrasound (CEUS) offers benefits in patients with kidney failure in the clinical setting including the use of a nonnephrotoxic intravascular contrast agent and the fact that it can be performed at the bedside in critical cases. Therefore, the aim of this study is to investigate whether CEUS can reliably identify typical imaging features of RCN. We retrospectively analyzed 12 patients with RCN examined in our department and confirmation of the diagnosis by either histopathology, other contrast-enhanced cross-sectional imaging tests, and/or CEUS follow-up. Assessed parameters in conventional US were reduced echogenicity, loss of corticomedullary differentiation, length and width of kidney, hypoechoic rim, resistance index and in CEUS delayed wash-in of contrast agent (> 20 s), reverse rim sign, maximum nonenhancing rim and additional renal infarction. Furthermore, imaging features in RCN were compared with the findings in renal vein thrombosis (RVT), among them echogenicity, corticomedullar differentiation, hypoechoic rim, RI value, delayed cortical enhancement, total loss of cortical perfusion and enhancement of renal medulla. All 12 patients showed the reverse rim sign, while a hypoechogenic subcapsular rim was only visible in four patients on B-mode ultrasound. A resistance index (RI) was available in 10 cases and was always less than 1. RI was a strong differentiator in separating RVT from RCN (RI > 1 or not measurable due to hypoperfusion as differentiator, p = 0.001). CEUS showed total loss of medullary enhancement in all cases of RVT. With its higher temporal resolution, CEUS allows dynamic assessment of renal macro- and microcirculation and identification of the typical imaging findings of RCN with use of a nonnephrotoxic contrast agent.
The toolish hand illusion: embodiment of a tool based on similarity with the hand
Authors: Cardinali Lucilla , Zanini Alessandro , Yanofsky Russell , Roy Alice C. , de Vignemont Frédérique , Culham Jody C. , Farnè Alessandro 
Published: January 2021

AbstractA tool can function as a body part yet not feel like one: Putting down a fork after dinner does not feel like losing a hand. However, studies show fake body-parts are embodied and experienced as parts of oneself. Typically, embodiment illusions have only been reported when the fake body-part visually resembles the real one. Here we reveal that participants can experience an illusion that a mechanical grabber, which looks scarcely like a hand, is part of their body. We found changes in three signatures of embodiment: the real hand’s perceived location, the feeling that the grabber belonged to the body, and autonomic responses to visible threats to the grabber. These findings show that artificial objects can become embodied even though they bear little visual resemblance to the hand.
Neurofilament light is a novel biomarker for mitochondrial encephalomyopathy, lactic acidosis, and stroke-like episodes
Authors: Zheng Yong-Sheng , Sun Chong , Wang Rong , Chen Ne , Luo Su-Shan , Xi Jian-Ying , Lu Jia-Hong , Zhao Chong-Bo , Li Yu-Xin , Zhou Lei , Lin Jie 
Published: January 2021

AbstractMitochondrial encephalomyopathy, lactic acidosis, and stroke-like episodes (MELAS) is a complicated maternally inherited disorder lacking of sensitive and specific biomarkers. The objective of this study was to investigate the serum neurofilament light chain (NfL) as a novel biomarker of neurological dysfunction in MELAS. Patients with different status of MELAS were enrolled in this study. The Mini-Mental State Examination (MMSE) was given to the participants to evaluate cognition status. Multiple functional MRI was performed on the participants. Blood samples were collected and the serum NfL concentrations were determined by the single-molecule array technology (Simoa). This study enrolled 23 patients with MELAS, 15 people in the acute attack phase of MELAS and 10 people in the remission phase, including 2 patients in both acute attack and remission phase. Sixteen healthy controls (HCs) were also enrolled. Serum NfL level increased significantly in patients with MELAS. Serum NfL level in the acute attack group (146.73 [120.91–411.31] pg/ml, median [IQR]) was higher than in the remission group (40.31 [19.54–151.05] pg/ml, median [IQR]) and HCs group (7.70 [6.13–9.78] pg/ml, median [IQR]) (p < 0.05). The level of NfL in the remission phase group was higher than in HCs group (p < 0.05). A negative correlation was found between the serum NfL level and MMSE (p = 0.006, r = -0.650). The NfL concentration correlated positively with stroke-like lesion volume in the brain (r = 0.740, p < 0.001). Serum NfL may serve as a novel biomarker for the neurological dysfunction in MELAS patients.
High expression of ACE2 and TMPRSS2 and clinical characteristics of COVID-19 in colorectal cancer patients
Authors: Liu Chao , Wang Kai , Zhang Min , Hu Xiaoyu , Hu Tian , Liu Yumei , Hu Qinyong , Wu Shikai , Yue Jinbo 
Published: January 2021

AbstractLittle is known of the patterns of expression of ACE2 and TMPRSS2 or the clinical characteristics of COVID-19 in patients with COVID-19 and colorectal cancer. We found in both bulk and single-cell RNA-seq profiles that ACE2 and TMPRSS2 were expressed at high levels on tumor and normal colorectal epithelial tissues. Clinically, patients with colorectal cancer and COVID-19 were more likely to have lymphopenia, higher respiratory rate, and high hypersensitive C-reactive protein levels than matched patients with COVID-19 but without cancer. These results suggest that patients with colorectal cancer may be particularly susceptible to SARS-CoV-2 infection. Further mechanistic studies are needed to support our findings.
Gene regulatory networks controlling differentiation, survival, and diversification of hypothalamic Lhx6-expressing GABAergic neurons
Authors: Kim Dong Won , Liu Kai , Wang Zoe Qianyi , Zhang Yi Stephanie , Bathini Abhijith , Brown Matthew P. , Lin Sonia Hao , Washington Parris Whitney , Sun Changyu , Lindtner Susan , Lee Bora , Wang Hong , Shimogori Tomomi , Rubenstein John L. R. , Blackshaw Seth 
Published: January 2021

AbstractGABAergic neurons of the hypothalamus regulate many innate behaviors, but little is known about the mechanisms that control their development. We previously identified hypothalamic neurons that express the LIM homeodomain transcription factor Lhx6, a master regulator of cortical interneuron development, as sleep-promoting. In contrast to telencephalic interneurons, hypothalamic Lhx6 neurons do not undergo long-distance tangential migration and do not express cortical interneuronal markers such as Pvalb. Here, we show that Lhx6 is necessary for the survival of hypothalamic neurons. Dlx1/2, Nkx2-2, and Nkx2-1 are each required for specification of spatially distinct subsets of hypothalamic Lhx6 neurons, and that Nkx2-2+/Lhx6+ neurons of the zona incerta are responsive to sleep pressure. We further identify multiple neuropeptides that are enriched in spatially segregated subsets of hypothalamic Lhx6 neurons, and that are distinct from those seen in cortical neurons. These findings identify common and divergent molecular mechanisms by which Lhx6 controls the development of GABAergic neurons in the hypothalamus.
Effect of an iOS App on Voice Therapy Adherence and Motivation
Authors: van Leer Eva , Lewis Brittney , Porcaro Nick 
Published: January 2021

Purpose
Patients commonly report difficulties adhering to voice therapy. An iOS app was developed in our lab that assists practice via reminder notifications, instructional recordings, and cepstral peak prominence analysis results. The purpose of this study was to assess the effect of such homework support modality on adherence behavior and associated motivation in a comparison of app support and written homework instructions and to assess the usability and utility of the app.

Method
Thirty-four individuals exhibiting adducted hyperfunction were randomized to receive either written homework instructions or the app when practicing resonant voice exercises for 3 weeks. All patients digitally audio-recorded all home practice, provided self-reported estimates of generalization, and completed weekly motivation scales.

Results
App support significantly increased practice frequency but did not affect self-reported generalization or motivation. Practice was significantly predicted by System Usability Scale scores. Utility of reminders and instructions were good, but cepstral peak prominence feedback was considered useful to only a subset of participants.

Conclusion
Interactive mobile therapy support can significantly increase practice of resonant voice homework without influencing motivation.
Restore DeepFakes video frames via identifying individual motion styles
Authors: Zhang Haichao , Lu Zhe‐Ming , Luo Hao , Feng Ya‐Pei 
Published: January 2021

AbstractRecent advance on highly realistic AI‐synthesised video approaches makes it hard to distinguish whether a speaker in video is real. Many existing DeepFakes detection approaches can be invalidated by using them as supervision in adversarial training, which in turn improves the performance of DeepFakes. This makes the problem a dilemma. However, human can recognise the identity of familiar persons by observing their motion styles. Inspired by this, the paper proposes a novel method to recognise original speaker's identity clues in DeepFakes videos by learning individual motion styles. As a biological signature, motion styles can neither be used in the training process of DeepFakes nor be modified to deceive detection methods without reducing realistic performance. Also, this paper proposes a novel pipeline to continuously restore the original frames from DeepFakes videos without knowing the DeepFakes approach in advance. Based on above ideas, our scheme makes it possible to restore DeepFakes videos. Through training the cross‐modal transfer module, the appearance embedding can be inferred from the identity code. Extensive experiments are conducted to reveal the effectiveness of the method, it shows for the first time that the original persons can be identified automatically and the original video from DeepFakes videos can be generated.
Bias stress instability in multilayered MoTe2 field effect transistors under DC and pulse‐mode operation
Authors: Seo Seung Gi , Jeong Jinheon , Kim Seung Yeob , Kim S. , Kim Kwangtaek , Kim Kyuwon , Jin Sung Hun 
Published: January 2021

AbstractHere, bias stress instability on multilayered MoTe2 field effect transistors (m‐MoTe2 FETs) with encapsulation of hydrophobic polymers (cyclic transparent optical polymer) is systematically investigated under DC and pulse mode operation. During DC mode stress, the threshold voltage shift (ΔVth) under positive bias stress is at least three times larger than that of negative stress due to high hole barrier (∼0.57 eV), compared with electron barrier (∼0.18 eV). However, ΔVth in positive pulse mode stress is significantly reduced with decrease of a duty cycle, as compared with that of negative stress. With isolation from external gas ambient effects such as H2O and O2, shallow electron traps in multilayered MoTe2 field effect transistors are identified as one of strong candidates to cause bias polarity dependency on ΔVth in pulse mode. Moreover, recovery time for traps, involved during positive bias stress, is six times faster than that of negative stress, substantiating that shallow electron traps and their fast detrapping are one of key origins. In practice, optimisation of pulse mode operation via bipolar switching can be potentially utilised for minimisation of device instability during the operation of multilayered MoTe2 field effect transistors devices and their circuits.