bluuebunny/crossref_metadata_2025_split huggingface dataset:
­­­­Optimal Glucose and Stress-hyperglycaemia Ratio Cut-off Values for Predicting 1-year Mortality in Diabetic and Non-diabetic Acute Myocardial Infarction Patients
Authors: Sia Ching-Hui , Chan Mervyn Huan-Hao , Zheng Huili , Ko Junsuk , Ho Andrew Fu-Wah , Chong Jun Hua , Foo David , Foo Ling-Li , Lim Patrick Zhan-Yun , Liew Boon Wah , Chai Ping , Yeo Tiong-Cheng , Tan Huay-Cheem , Chua Terrance , Chan Mark Yan-Yee , Tan Jack Wei Chieh , Bulluck Heerajnarain , Hausenloy Derek J 
Published: August 2021

Abstract
Background

Stress-induced hyperglycaemia at time of hospital admission has been linked to worse prognosis following acute myocardial infarction (AMI). The stress-hyperglycaemia ratio (SHR) index normalises the acute increase in blood glucose values to background glycaemic status. However, the optimal cut-off blood glucose and SHR values for predicting adverse outcomes post-AMI are unknown. As such, we determined the optimal blood glucose and SHR cut-offs for predicting 1-year all cause mortality in diabetic and non-diabetic non-ST-segment elevation myocardial infarction (NSTEMI) and ST-segment elevation myocardial infarction (STEMI) patients.
Methods

We undertook a national, registry-based study of patients with AMI from January 2008 to December 2015. We determined the optimal blood glucose and SHR cut-off values using the Youden’s formula for 1-year all-cause mortality. We subsequently analyzed the sensitivity, specificity, positive and negative predictive values of the cut-offs in the diabetic and non-diabetic subgroups, stratified by the type of AMI.
Results

There were 5,841 STEMI and 4,105 NSTEMI in the study. In STEMI patients, both glucose and SHR were independent predictors of 1-year all-cause mortality [Glucose: OR 2.19 (95% CI 1.74–2.75); SHR: 2.19 (95% CI 1.73–2.78)]. However, in NSTEMI patients, glucose and SHR were not independently associated with 1-year all-cause mortality [Glucose: OR 1.37 (95% CI 1.00-1.89); SHR: 1.27 (95% CI 0.91–1.78)]. In STEMI patients, ROC analysis showed that SHR performed better than glucose (AUC for glucose 0.633 versus AUC for SHR 0.692, P < 0.001) in diabetic patients, whereas in non-diabetic patients, SHR and glucose performed equally well (AUC for glucose 0.720 versus AUC for SHR 0.717, P < 0.664). The optimal glucose cut-off values were 15.0mmol/L for diabetic STEMI patients and 11mmol/L for non-diabetic STEMI patients and the corresponding optimal cut-off values for SHR were 1.7 and 1.5, respectively.
Conclusions

Glucose on admission and SHR were independent predictors of 1-year all-cause mortality in STEMI, whereas this was not the case in NSTEMI patients. In STEMI setting, SHR performed better than admission glucose to predict 1-year all-cause mortality in diabetic patients, whereas in non-diabetic patients both SHR and glucose performed equally well.
Mitochondrial Fission Regulator 2 (MTFR2) Confers anti-ER Endocrine Therapy Resistance to Breast Cancer Cells by Modulating Mitochondrial Autophagy through Direct Phosphorylation of FUNDC1
Authors: Zhang Zhanqiang , Lu Guanming , Lin Bo , Li Yanghong , Li Fuju , Wen Zipeng , Lv Weiming , Lai Yuanhui , li jie 
Published: August 2021

Abstract
Background: Mitochondrial fission regulator 2 (MTFR2) belongs to the MTFR1/ family with sequence similarity 54 (FAM54) family. Recently, it was reported that MTFR2 promotes the proliferation and invasion of breast cancers (BCs). However, the relationship between MTFR2 and endocrine crine therapy resistance is still unknown.Materials and methods: We collected 36 ER+ BC tissues and adjacent normal tissues and 10 samples from patients who received endocrine therapy. We detected the expression pattern of MTFR2 and the fold change in MTFR2 in cells treated with tamoxifen and letrozole. The autophagy status was also determined.Results: MTFR2 expression was upregulated in ER+ BC tissues and was strongly upregulated in the samples that were treated with endocrine therapy. Knocking out MTFR2 increased BC cell sensitivity to endocrine therapy. In addition, MTFR2 directly bound to FUNDC1 and promoted FUNDC1 phosphorylation, thus inhibiting autophagy.Conclusion: Taken together, our results indicate that increased expression of MTFR2 is associated with endocrine therapy resistance in BC cells. Our findings indicate that MTFR2 could serve as a novel therapeutic target for ER+ BC patients who suffer from endocrine therapy resistance.
Cost-Effectiveness of Remdesivir Plus Supportive Care for Patients with COVID-19 in Iran
Authors: Gholamhoseini Mohammad Tasavon , Goudarzi Reza , Yazdi-Feyzabadi Vahid , Mehrolhassani Mohammad Hossein , Yousefi Meysam 
Published: August 2021

Abstract
Background: Remdesivir is a medication used for moderate to severe Coronavirus disease 2019 (COVID-19) patients with favorable effects. However, it is an expensive medication. Therefore, the present study aimed to assess the cost-effectiveness of remdesivir plus supportive care (SC) for COVID-19 patients in Iran.Methods: Markov model was used to compare costs and quality-adjusted life-days (QALDs) of remdesivir+SC and SC for patients with COVID-19. The model simulated a cycle length of one day and a 30-day time horizonin TreeAge 2020 software. The costs from the healthcare system perspective were obtained from Afzalipour hospital as a referral hospital for the hospitalization of COVID-19 patients in Kerman, Iran. All the costs were converted to 2018 purchasing power parity (PPP) US dollars. Utility values were derived from published sources. The results were presented as an incremental cost-effectiveness ratio (ICER) at a willingness-to-pay (WTP) threshold of three times the Gross Domestic Product per capita of Iran. Both deterministic and probabilistic sensitivity analyses were performed.Results: The base-case results showed that the treatment of COVID-19 patients with remdesivir+SC had a cost of 8795 PPP US dollars for 21.13 QALD gained. The SC alone cost 8637 PPP US dollars with a gain of 20.20 QALD. Our findings demonstrated that at a WTP threshold of 159 PPP US dollars per QALD, remdesivir+SC was not cost-effective with an ICER of 168 PPP US dollars per QALD. Deterministic sensitivity analysis indicated ICER to be sensitive to the transition probabilities and costs. Probabilistic sensitivity analysis revealed that remdesivir+SC was cost-effective at a WTP of 159 PPP US dollars per QALD in 47% of iterations.Conclusions: Our findings demonstrated thatremdesivir+SC is not cost-effective, compared to SC alone. Considering the lack of studies on the effectiveness of remdesivir, the findings should be interpreted with caution. Further evaluations are recommended to determine the efficacy and effectiveness of remdesivir in COVID-19 patients.
Changes of Gait Characteristics in Stroke Patient with Foot Drop After Treating by Foot Drop Stimulator Combined with Moving Treadmill Training
Authors: CHEN Peishun , ZHOU Haiwang , LI Taotao , GUANG Hongli , MIN Yu , ZHANG Wanrong 
Published: August 2021

Abstract
Background and PurposeTo analyze the changes of gait characteristics in stroke patient with foot drop after treating by foot drop stimulator combined with moving treadmill, and provide a basis for the improvement of foot drop gait after stroke.Methods Sixty patients with stroke and hemiplegia with foot drop were randomly divided into the experimental group and the control group. Both groups received basic rehabilitation training. On this basis, the experimental group received foot drop stimulator combined with moving treadmil. The control group received foot drop stimulator training. The treatment time of the two groups was 30 minutes/time, 5 times a week, for 3 consecutive weeks. Gait Watch three-dimensional gait analysis system was used to record the angle of hip flexion, knee flexion and ankle flexion before and after treatment. Results After treatment, the angles of hip flexion, knee flexion, and ankle flexion of the two groups of patients changed, but the above changes in the experimental group were better than those in the control group, The difference is statistically significant (p<0.05). ConclusionsThe foot drop stimulator combined with moving treadmill can significantly improve the foot gait after stroke, and promote the normalization of hip flexion and knee flexion.
Antitumor Effects of Cadmium Against Diethylnitrosamine-induced Liver Tumors in Mice
Authors: Nie Yu , Huang Bo , Hu An-Ling , Xu Yun-Yan , Zou Yan , Liu Yun , Liu Jie 
Published: August 2021

Abstract
Background: Cadmium (Cd) is reported to have antitumor effects against chemical-induced liver tumors. Antitumor effects of Cd are not completely understood, but may be related to metallothionein-deficiency in tumors, which makes tumor vulnerable to necrotic effects of Cd. Methods: Eight-week old male C57BL/6 mice were given injections of diethylnitrosamine (DEN, 90 mg/kg, and 50 mg/kg two weeks later), followed by promotion with CCl4. CdCl2 was administered in the drinking water (500 ppm) from 21-40 weeks after DEN initiation. Body weights were recorded and liver tumor formation was monitored via ultrasound. At the end of experiments, livers were removed, weighed, and the tumor incidence, tumor numbers and tumor size scores were recorded. Liver histology and metallothionein immunostaining were performed. Results: After DEN injection, animal body weight decreased, and slowly recovered afterwards. Cd treatment did not affect animal body weight gain. Ultrasounds detected liver tumors 35 weeks after DEN injection. Animals were necropsied at 40 weeks. Liver/body weight ratios increased in DEN and DEN+Cd groups. Cd treatment decreased tumor incidence (71% vs 17%), tumor numbers (15 vs 2), and tumor scores (22 vs 3). Histopathology showed hepatocyte degeneration in all groups, and immunohistochemistry showed metallothionein-deficiency in liver tumors, while metallothionein stain was intensified in tumor surrounding tissues. RT-qPCR showed increases of alpha-fetoprotein in DEN-treated livers, and increases of metallionein-2 and TNFα in Cd-treated livers. Conclusion: Cd is effective in suppression of DEN-induced liver tumors, and the mechanisms may be related to metallothionein-deficiency in tumors and induction of TNFα to kill tumor cells.
Influence of Prior Authorization Requirements on Provider Clinical Decision Making
Authors: Salzbrenner Stephen G. , Lydiatt Maxwell , Heldin Brandon , Scheier Lawrence M. , Hill Trish Wonch , Greene Harrison , McAdam-Marx Carrie 
Published: August 2021

Abstract
Background: Prior authorization (PA) of medications is widely used by payers in the United States as a way to promote safe and effective use of medications and to control costs. However, PA-related tasks such as completing forms, submitting forms, researching medical history and submitting required documentation can all contribute to burden on healthcare providers. This study examines how such tasks and affect provider burden and treatment decisions. Methods: We developed and administered a nationwide, cross-sectional online survey of medical providers in the United States in 2020 based on a convenience sample of 100,000 providers (physicians, nurse practitioners, and physician assistants). Path analysis was used to test the associations between provider practice characteristics, step therapy and other health plan requirements, perceived burdens of PA, and communication issues with insurers on prescribing behaviors, which included prescribing a different medication, avoiding prescribing of newer medications, and modifying a diagnosis. Weighted analyses were also conducted to account for sample bias due to non-response. Results: A total of 1173 providers (1.2% response rate) provided 1147 usable surveys. The sample was 49.6% female, and a majority were MD/DO providers (85%). Step therapy requirements had the largest influence on prescribing a different medication than planned (b = .22, 95% CI = .160-.285) and avoiding prescribing a newer medication despite meeting evidence-based guidelines (b = .24, 95% CI = .181-.309). A unit-weighted index of perceived PA burden risk was associated with prescribing a different medication (b = .09, 95% CI = .012-.128) and modifying a diagnosis to obtain PA approval (b = .14, 95% CI = .065-.195). Communication issues were associated with prescribing a different medication (b = .11, 95% CI = .029-.186), while health plan requirements (e.g., clinical documentation) was significantly associated with all three prescribing outcomes. Weighted analyses showed that the study conclusions were unlikely to have been biased by nonresponse. Conclusions: Providers report altering prescribing and modifying diagnoses to avoid PA requirements and related burdens. Processes that reduce the administrative burden of PA through improved communication and transparency as well as standardized documentation may help ensure that PA more seamlessly achieves its goals of safe and effective use of medications. Trial Registration: NA Keywords: clinical decision making, health plan, prior authorization, provider burden, specialty types, workaroundsTrial Registration: NA
Modelling Hydrometeorological Extremes Associated to the Moisture Transport Driven by the Great Plains Low-Level Jet
Authors: Gimeno-Sotelo Luis , Bermudez Patricia de Zea , Algarra Iago , Gimeno Luis 
Published: August 2021

Abstract
The Great Plains Low-Level Jet system consists of very strong winds in the lower troposphere that transport a huge amount of moisture from the Gulf of Mexico to the American Great Plains. This paper aims to study the extremes of the Transported Moisture (TM) from the GPLLJ source region to the jet domain; and, for low and high TM, to analyze the extremal dependence between the upper tail of the precipitation in the GPLLJ sink region and the lower tail of the tropospheric stability in that region (omega).
The declustered extremes of TM were analyzed using Peaks Over Threshold (POT). A non-stationary Exponential model was fitted to the cluster maxima. Estimated return levels show that the extremes of TM are expected to decrease in the future. This is meteorologically congruent with the known displacement of the western edge of the North Atlantic Subtropical High, which controls atmospheric circulation in the North Atlantic, and to a higher scale with the change of phase from negative to positive of the Atlantic Multidecadal Oscillation.
Bilogistic and Logistic models were fitted to the extremes of (-omega, precipitation) for low and high TM, respectively. The extremal dependence between "-omega" and precipitation proves to be stronger in the case of high TM. This confirms that dynamical instability represented by “-omega” is the most important parameter for achieving high values of precipitation once there is a mechanism that allows the continuous supply of large amounts of moisture, such as the derived from a low-level jet system.
Integrated Weighted Gene Co-expression Network Analysis Reveals ALB Associated With Prognosis of High-grade Serous Ovarian Cancer
Authors: Wang Bo , Chao Shan , Guo Bo 
Published: August 2021

Abstract
Background: Ovarian cancer is the gynecologic tumor with the highest fatality rate, and high-grade serous ovarian cancer (HGSOC) is the most common and malignant type of ovarian cancer. One important reason for the poor prognosis of HGSOC is the lack of effective diagnostic and prognostic biomarkers. New biomarkers are necessary for improvement of treatment strategies and to ensure appropriate healthcare decisions.Methods: To construct the co-expression network of HGSOC samples, we applied weighted gene co-expression network analysis (WGCNA) to assess the proteomic data downloaded from Clinical Proteomic Tumor Analysis Consortium (CPTAC), and module-trait relationship was then analyzed and plotted in a heat map to choose key module associated with HGSOC. Enrichment analysis was performed on the genes in the key modules to explore the functional information in which these genes participate. Hub genes with high connectivity in key module were identified by Cytoscape software. Furthermore, the true hub gene was selected through survival analysis, followed by expression verification with transcriptome dataset from TCGA and GTEx. Finally, the potential biological functions of hub gene were analyzed via single-gene Gene Set Enrichment Analysis (GSEA).Results: After merging similar modules, a total of 9 modules were identified. Module-trait analysis revealed that the brown module (cor = 0.7) was significantly associated with HGSOC. The results of enrichment analysis of the genes in the brown module show that these genes were related to the functions of the extracellular matrix, the complement system and the blood system. Ten hub genes with the highest connectivity were selected by protein-protein interaction analysis. After survival analysis and expression verification of hub genes, only ALB was confirmed to be the true hub gene and positively correlated with HGSOC prognosis. Single gene GSEA revealed that ALB was associated with cell material degradation.Conclusion: We conducted the first gene co-expression analysis based on proteomic data from HGSOC samples, and found that ALB had prognostic value, which could be applied in the treatment of HGSOC in the future.
Content validation of observer-reported sickle cell pain diaries (SCPD-CS and SCPD-CN): results from interviews with caregivers
Authors: White Michelle , Foster April , Bailey Miranda , D'Alessio Denise , Rizio Avery , Stebbins Patricia , Pierre Danielle St. , Saucier Cory 
Published: August 2021

Abstract
Background
Patients with sickle cell disease (SCD) experience daily pain and acute episodes known as sickle cell pain crises (SCPCs). The Sickle Cell Pain Diary - Caregiver Report (SCPD-C) is an observer-reported diary for use by caregivers of children ages < 12 years with SCD. This study reports on the content validity of the SCPD-C.
Methods
The SCPD-C was developed based on a literature review, measurement expert input, and a patient advisory board including clinicians. Three rounds of interviews (including both concept elicitation and cognitive debriefing methodologies) were conducted with caregivers of children with SCD aged < 12 to evaluate the content validity of the SCPD-C.
Results
Across three rounds of interviews, caregivers confirmed concepts in the SCPD-C and described observed impacts that were important and were added. Overall, caregivers evaluated the SCPD-C as easy to understand, with some minor adaptations for clarity. Additionally, the diary was split into two versions based on the child’s age and school enrollment status (SCPD-CS for school-aged and SCPD-CN for non-school age children).
Conclusions
Caregivers provided valuable input that led to important additions and changes to the measures. The SCPD-CS and SCPD-CN are appropriate and fit-for-purpose observer-reported outcome measures of SCPC-related pain frequency and severity, and the impacts on health-related quality of life.
Prenatal Prognostic Factors for Isolated Right Congenital Diaphragmatic Hernia: A Single Center’s Experience
Authors: Jeong Jiyoon , Lee Byong Sop , Cha Teahyen , Jung Euiseok , Kim Ellen Ai-Rhan , Kim Ki-Soo , Kim Dae Yeon , Namgoong Jung-Man , Kim Seong-Chul , Lee Mi-Young , Won Hye-Sung 
Published: August 2021

Abstract
Background: Right-sided congenital diaphragmatic hernia (RCDH) is relatively rare. Clinical data of RCDH, especially with respect to antenatal prediction of neonatal outcome, are lacking. This study aimed to report the treatment outcome of newborn infants with RCDH and to identify prenatal prognostic indicators. Methods: We retrospectively reviewed the medical records of newborn infants with isolated RCDH who were born at a gestational age of ≥35 weeks. We analyzed and compared the clinical and prenatal characteristics including the fetal lung volume, which was measured as the observed-to-expected lung area-to-head circumference ratio (O/E LHR), between the survivors and non-survivors. Results: A total of 24 (70.6%) of 34 patients with isolated RCDH survived to discharge. The O/E LHR was significantly greater in survivors (67.0 ± 19.9) than in non-survivors (42.4 ± 25.0) (P < .05). In multivariate analysis, the O/E LHR was found to be a good predictor of mortality or requirement for extracorporeal membrane oxygenation (ECMO) support (area under the curve [AUC], 0.870; P < .01) and mortality (AUC, 0.789; P < .05). The best O/E LHR cut-off value for predicting mortality and mortality or requirement for ECMO support in isolated RCDH was 47. Conclusions: The survival rate of patients with isolated RCDH was comparable to that of patients with left-sided CDH. The O/E LHR of the contralateral lung was a reliable predictor of short-term postnatal outcome in RCDH.
Development of Improved Cellulase Variants for the Conversion of Spent Mushroom Substrate Supplemented with Wheat Straw by Directed Evolution
Authors: Mauriello Valentina , Pennacchio Anna , Dallo Irantzu Alegria , Saez Laura Garcia , Ihalainen Petri , Mialon Antoine , Ventorino Valeria , Faraco Vincenza 
Published: August 2021

Abstract
To improve the Spent mushroom substrate (SMS) saccharification, cloning, recombinant expression in Escherichia coli and characterization of two new GH5 family cellulases (Cel1 and Cel2) were performed. Based on enzymes properties, Cel2 was selected for the generation of 30,000 random mutants by directed evolution in order to develop improved biocatalysts. Error-prone Polymerase Chain Reaction was used for diversity generation in cel2 gene and the screening for activity of mutants allowed selection of 63 improved variants that were subjected to a scale up production. Among these, 13 clones exhibited two-fold higher activity than Cel2 and a higher thermoresistance after 72h. The performances of these mutants in the hydrolysis of pretreated SMS/ wheat straw (40/60) were compared to the wild type Cel2 in conjunction with a commercial enzymatic mixture (MetZyme® SUNO™ BOOSTER 144). All the mutants exhibited a glucose yield two-fold or four fold higher than wild-type Cel2 after 72h of incubation.
Effect of Ligninolytic Axenic and Coculture White-Rot Fungi on Rice Straw Chemical and In Vitro Fermentation Characteristics.
Authors: Datsomor Osmond , Gou-qi Zhao , Miao Lin 
Published: August 2021

Abstract
The objective of this study was to investigate the axenic culture of Pleurotus ostreatus, Phanerochaete chrysosporium, and the coculture (P. chrysosporium and P. ostreatus) for their potential to break down lignin and to enhance the rumen fermentability of rice straw. Rice straw was fermented by two lignin-degrading fungi, namely, P. ostreatus, P. chrysosporium, and coculture (P. ostreatus and P. chrysosporium) under solid-state fermentation (SSF). The coculture exhibited a mutual intermingling plus inhibition interaction. Coculture treated straw had a lower lignin content (5.26%) compared to P. chrysosporium axenic treated straw (6.18%), although P. ostreatus axenic treated straw was the lowest (3.27%). The polysaccharide content of coculture treated straw was higher than P. chrysosporium axenic treated straw, although smaller than P. ostreatus. P. ostreatus and coculture suitably delignified rice straw without adversely affecting cellulose. Treatment of rice straw with coculture improved in vitro dry matter digestibility (68.08%), total volatile fatty acids (35.27%), and total gas (57.4 ml/200 mg) compared to P. chrysosporium (45.09%, 32.24%, 44.39 ml/200 mg) but was second to P. ostreatus (75.34%, 38.31%, 65.60 ml/200 mg). The coculture via synergistic interaction has the potential to decompose lignin and improve the nutritive value of rice straw than P. chrysosporium.