bluuebunny/crossref_metadata_2025_split huggingface dataset:
Presenilin/γ-Secretase Activity Is Located in Acidic Compartments of Live Neurons
Authors: Maesako Masato , Houser Mei C. Q. , Turchyna Yuliia , Wolfe Michael S. , Berezovska Oksana 
Published: November 2021

Presenilin (PSEN)/γ-secretase is a protease complex responsible for the proteolytic processing of numerous substrates. These substrates include the amyloid precursor protein (APP), the cleavage of which by γ-secretase results in the production of β-amyloid (Aβ) peptides. However, exactly where within the neuron γ-secretase processes APP C99 to generate Aβ and APP intracellular domain (AICD) is still not fully understood. Here, we employ novel Förster resonance energy transfer (FRET)-based multiplexed imaging assays to directly “visualize” the subcellular compartment(s) in which γ-secretase primarily cleaves C99 in mouse cortex primary neurons (from both male and female embryos). Our results demonstrate that γ-secretase processes C99 mainly in LysoTracker-positive low-pH compartments. Using a new immunostaining protocol which distinguishes Aβ from C99, we also show that intracellular Aβ is significantly accumulated in the same subcellular loci. Furthermore, we found functional correlation between the endo-lysosomal pH and cellular γ-secretase activity. Taken together, our findings are consistent with Aβ being produced from C99 by γ-secretase within acidic compartments such as lysosomes and late endosomes in living neurons.SIGNIFICANCE STATEMENTAlzheimer's disease (AD) genetics and histopathology highlight the importance of amyloid precursor protein (APP) processing by γ-secretase in pathogenesis. For the first time, this study has enabled us to directly “visualize” that γ-secretase processes C99 mainly in acidic compartments such as late endosomes and lysosomes in live neurons. Furthermore, we uncovered that intracellular β-amyloid (Aβ) is significantly accumulated in the same subcellular loci. Emerging evidence proposes the great importance of the endo-lysosomal pathway in mechanisms of misfolded proteins propagation (e.g., Tau, α-Syn). Therefore, the predominant processing of C99 and enrichment of Aβ in late endosomes and lysosomes may be critical events in the molecular cascade leading to AD.
“There is No End”: Anna Akhmatova’s Unfinished Tragedy Prologue
Authors: Poberezkina Polina 
Published: November 2021

The tragedy Prologue written in 1960s is analyzed in the broad context of the history of its creation, Anna Akhmatova’s biographical myth and world culture. The article mentions the problems of academic publication of the text and presents materials for scientific commentary on it. The incompleteness of the play is viewed in connection with its heterogeneous character combining lyrics, drama and epos, verses and prose, tragic and comic, ancient and modern, East and West, national and global, culture and everyday life, written and oral styles.
Woman at the Ball. “Anna in the Neck” by Anton Chekhov and “The Psychology of a Husband from Syros” by Emmanuel Roidis: An Attempt of Comparison
Authors: Eloeva Fatima 
Published: November 2021

The paper presents the first attempt to compare two short stories – Anna in the Neck by Anton Chekhov and a well-known short story by the Greek author Emmanuel Roidis Psychology of a Husband from Syros. Both texts were published at the same time (in 1895) and are considered to be masterpieces. The initial motivation for such textual analysis became almost a complete coincidence between the plots of the two stories. A series of telling coincidences and contradictions observed between the analyzed stories require some kind of explanation which will allow us to view both texts from the different angle. The paper aims to determine the correlation between the writers’ biographies and the personality traits with the characteristics of the protagonists and the style of story telling.
Virgo-Death: from The Little Show-Booth by Alexander Blok to The Mistress of Death and The Lover of Death by Boris Akunin
Authors: Fedunina Olga 
Published: November 2021

The image of Death, embodied in the image of a beautiful maiden, is considered in the article through the analysis of references in the novel diptych by B. Akunin The Mistress of Death and The Lover of Death (the Erast Fandorin series) to one of the most important primary sources, the drama by A. Blok The Little Show-Booth. The study shows that Akunin's method of deformation was replaced by a postmodern deconstruction with a splitting into two images, of Columbine and of Maiden-Death, each of which is dominated by one of the hypostases of the heroine of The Little Show-Booth. These transformations appeal in their development to the opposition in Akunin’s novels of two points of view on fate, dialectically interacting, which correlate with the adventurous exposition and with the inevitability of personal destiny idea, oriented towards the “classical” tradition. The result of the analysis is a new formula of the genre of Akunin's novels, since their poetics goes out of the ordinary framework of criminal literature, as a transgressive phenomenon in the field of mass literature, as postmodern novel, in which the uncertain intertextuality accentuates, align with plot details, the problem of heroes’ self-identity.
Sistem Pengaturan Pulse Width Modulation Motor Pada Robot Pembawa Makanan atau Minuman Menggunakan Joystick
Authors: Ramadhan Andika Enggal , Djuniadi Djuniadi , Apriaskar Esa 
Published: November 2021

Teknologi robotika kini sudah semakin berkembang pesat, hal ini ditunjukkan banyak sekali penggunaan teknologi robot yang digunakan dalam kehidupan sehari-hari untuk membantu pekerjaan manusia. Robot pengantar makanan atau minuman juga menjadi salah satu teknologi robotika yang kini semakin marak dikembangkan. Banyak peneliti yang sudah mulai mengembangkan robot sebagai alat pembantu pekerjaan manusia untuk pengantar makanan dan minuman. Pada robot tersebut, proses untuk mengatur kecepatan roda penggeraknya dapat menggunakan kontrol langsung atau kontrol tidak langsung. Pada penelitian ini, pengontrolan secara tidak langsung dilakukan menggunakan joystick untuk mengatur masukan pulse width modulation yang kemudian dikonversikan menjadi kecepatan robot. Pengaturan ini dibantu dengan mikrokontorller ATMEGA 328P sebagai alat untuk komputasi dan eksekusi jari program. Sehingga kecepatan robot dapat diatur dengan sempurna. Berdasarkan hasil pengujian diperoleh bahwa perubahan PWM awal pada saat robot dari kondisi diam menuju berjalan dengan perubahan kecepatan yang linier terhadap perubahan sinyal analog dari joystick, sehingga pergerakan robot lebih stabil. Nowdays, robotics was growing rapidly, it is widely used in everyday life to help human work. Food or beverage delivery robots are also one of the robotics technologies that are increasingly being developed today. Many developers has started to develop food or beverage delivery robots. In order to adjust the speed of the robots, we can use direct or indirect control. This study uses indirect control using joystick as a regulator of pulse width modulation which is converted to robot speed. Furthermore,, we used ATMEGA 328P microcontroller for computing and finger execution of the program. So that the speed of the robot can be adjusted perfectly. The result show that the initial PWM changes when the position of robot is rest to walking has linear change in speed to changes in the analog signal from the joystick, so that the robot's movement is more stable.
New species of Arge Schrank, 1802 (Hymenoptera, Argidae) from Turkey
Authors: Kaplan Emin , Haris Attila 
Published: November 2021

Arge yildirimi sp. n. is described from Turkey, Diyarbakir Province and compared to Arge pectoralis (Leach, 1817) and Arge frivaldszkyi (Tischbein, 1852).
A new species of Ophiocaryon (Sabiaceae) from the Amazonian slope of the eastern Cordillera of Colombia
Authors: Aymard C. Gerardo A. 
Published: November 2021

Ophiocaryon nicolasii, from the “Camino de los Andaquíes,” in the Amazonian slope of the Eastern Cordillera of Colombia, Caquetá department, is described and illustrated and its morphological relationships with other similar Ophiocaryon species are discussed. This new species is a small tree from the foothills to montane forest (500–1000 m). Morphologically it is related to O. manausense (W.A. Rodrigues) Barneby, but it differs by: smaller leaves, petioles, petiolules, and inflorescences; leaflets with 8–16 secondary veins; leaflets, bracteoles, staminodes and ovary with different shape; margins of sepals erose. Information about the Andakí region and its ancestral inhabitants is presented. An updated key to the species of Ophiocaryon is provided.
Observations on anthesis, fruit development, and seed dispersal in Gordonia lasianthus (Theaceae)
Authors: Brown Herrick H.K. , Wethey David S. 
Published: November 2021

The development of the flowers and fruits of loblolly bay (Gordonia lasianthus) is described in detail. Tests of meteorological conditions affecting the opening and closing of mature capsules reveals that seeds are typically released only when relative humidity falls below a critical threshold of 67%. Seedfall tests indicate that differences in the expressed aerodynamic pattern may account for variation in observed seedfall densities and affect horizontal displacement.
First report of a strict dragon tree species (Dracaena cambodiana: Asparagaceae) from India
Authors: Sarma Jatindra , Barbhuiya Hussain Ahmed , Dey Santanu 
Published: November 2021

Dracaena cambodiana Pierre ex Gagnep., a dragon tree species, is reported here for the first time from India. Its taxonomic relationships with other closely related species of genus Dracaena Vand. ex L. are examined and discussed. A detailed taxonomic description, along with color photographs is provided to facilitate its future identification. Recent surveys confirm that the habitat and regional population of the plant is under severe threat. Hence, following IUCN Red List Categories and Criteria, it is regionally categorized as Critically Endangered.
Notes on several Asian species of Styrax series Cyrta (Styracaceae)
Authors: Li Gary , Fritsch Peter W. 
Published: November 2021

Geographic range extensions are documented for the Asian species Styrax japonicus and S. odoratissimus of S. series Cyrta. Implications of the range extension in S. japonicus for the taxonomic distinctness and phylogenetic relationships of S. formosanus are discussed.
Exalloanthum, a new name for a fossil angiosperm flower in Myanmar amber
Authors: Poinar, Jr. George O. 
Published: November 2021

In a recent article, a flower from Burmese amber was described as Diaphoranthus burmensis. It was later discovered that the genus Diaphorathus Meyen had been used previously for an asterid, Diaphoranthus fuscus (Meyen 1834). The new name for Diaphoranthus burmensis is changed below to Exalloanthum burmense.
Geniş boyunlu paraoftalmik anevrizmaların akım çevirici stent implantasyonu ile endovasküler tedavisi
Authors: GÜZELDAĞ Hamit , ONAN Bilen , PİŞKİN Ferhat Can , SÖZÜTOK Sinan 
Published: November 2021

Amaç: Bu çalışmada, amacımız geniş boyunlu paraoftalmik anevrizmaların tedavisi için kullanılan akım çevirici stentlerin erken ve orta dönem klinik sonuçlarını sunmak idi.
Gereç ve Yöntem: Hastanemizin girişimsel radyoloji ünitesinde Ekim 2008-Ocak 2020 tarihleri arasında internal karotid arterin paraoftalmik segmentinde yer alan geniş boyunlu anevrizma nedeniyle akım çevirici stent ile tedavi edilen toplam 87 hastanın verileri retrospektif olarak analiz edildi.
Bulgular: Bu çalışmada yaş ortalaması 51,1 ± 8 olan 66’sı (% 75,8) kadın toplam 87 hasta yer aldı. Otuz dört (% 39) hastada birden fazla olmak üzere, toplam 100 paraoftalmik segment yerleşimli anevrizma, akım çevirici stentler ile tedavi edildi. Kırk (% 40) paraoftalmik segment anevrizmanın tedavisi için akım çevirici stente ek olarak koil embolizasyonu da yapıldı. Tüm stentler istenilen lokalizasyona yerleştirildi (Teknik başarı % 100). Bir hastada tedaviden sonra 5. günde intrakraniyal kanama nedeniyle ölüm gerçekleşti (Mortalite oranı: % 1,1). Uzun dönem takiplerde 3 hastada ileri düzeyde (stent lümenin %50 nin üzerinde daralaması) stent stenozu gelişti ve tedavi gerektirdi. Hastalar ortalama 29,8 ± 3 ay takip edildi. Yapılan kontrollerde ilk 3-6. ayda 77 (%81,9) anevrizma ve 6. ay sonrası kontrollerde 88 (%92,6) anevrizma kapalı idi.
Sonuç: Paraoftalmik anevrizmaların endovasküler tedavisinde akım çevirici stent implantasyonu tek başına veya koil embolizasyonu ile birlikte güvenilir ve etkin bir yöntemdir.
Surface Slip Distribution and Earthquake Rupture Model of the Fuyun Fault, China, Based on High-Resolution Topographic Data
Authors: Liang Zihan , Wei Zhanyu , Sun Wen , Zhuang Qitian 
Published: November 2021

Abstract
The characteristics of earthquake surface ruptures, such as geometry, slip distribution, and coseismic deformation, contain important information about the earthquake rupture process, and so investigations and analyses of earthquake surface rupture have played a crucial role in modern earthquake hazard studies, especially with the increasing availability of high-resolution topographic and imagery data for tectono-geomorphic interpretation. In this study, we use Structure from Motion (SfM) photogrammetry to build a 1 m resolution digital elevation model (DEM) of the fault and combine this with filed observations to map the surface ruptures of the 1931 M8.0 Fuyun earthquake, China. These high-resolution topographic data enable to identify and measure the displaced gullies, and so the rupture locations and along-strike slip distribution are obtained in detail. Four paleoearthquake events are identified through the offset cluster characteristics. The coseismic offset of the 1931 Fuyun earthquake is extracted from the offset distribution, which shows four continuous undulations along the fault strike, corresponding to the four segments of surface rupture. Moreover, a high offset gradient is observed in the step area connected by the rupture segment. These findings, combined with the width and bending angle of the step area at the joint of the rupture segment, indicate that the 1931 Fuyun earthquake was a cascade rupture formed by four rupture segments. This study expands the available offset measurement data of Fuyun fault and confirms the applicability of high-resolution topographic data to active tectonic research.
Integrated Mechanical Earth Modeling for Predicting Sand Production: A Case Study
Authors: Al-Ameri Aymen 
Published: November 2021

Summary
Sand production is a serious problem in oil and gas wells, and one of the main concerns of production engineers. This problem can damage downhole equipment and surface production facilities. This study presents a sand production case and quantifies sanding risks for an oil field in Iraq.
The study applies an integrated workflow of constructing 1D Mechanical Earth Modeling (MEM) and predicting the sand production with multiple criteria such as shear failure during drilling, B index, and critical bottomhole pressure (CBHP) or critical drawdown pressure (CDDP). Wireline log data were used to estimate the mechanical properties of the formations in the field. The predicted sand production propensity was validated based on the sand production history in the field.
The interpretation results of some wells anticipated in this study showed that when a shear failure occurs during drilling, the B index is around 2 × 104 MPa or less and the CBHP is equal to the formation pore pressure. For this case, sand control shall be carried out in the initial stage of production. On the other hand, when the shear failure does not exist, the B index is always greater than 2 × 104 MPa, and the CBHP is mostly less than the formation pore pressure. In this case, implementing sand control methods could be postponed as the reservoir pressure undergoes depletion. However, for the anticipated field, sand control is recommended to be carried out in the initial stage of well production even when the CBHP is less than the formation pore pressure since sanding will be inevitable when the reservoir pressure depletes to values close to the initial reservoir pressure.
The tentative evaluation of the stress regime showed that a normal fault could be the stress regime for the formations. For a normal fault stress regime, the study explained that when the reservoir permeability is isotropic, an openhole vertical wellbore has less propensity for sand production than a horizontal wellbore. Moreover, when the wellbore azimuth is in the direction of the minimum horizontal stress, the CBHP will be lower than in any other azimuth, and sanding will take place at higher wellbore inclination angles. For the anticipated field, because of the casedhole well completion and the anisotropic reservoir permeability, a horizontal well drilled in the direction of minimum horizontal stress with oriented perforation in the direction of maximum horizontal stress is an alternative method for controlling sand production.
Bioinformatics analysis of NSCLC multi-omics data
Authors: Lehtiö Janne , Arslan Taner , Siavelis Ioannis , Pan Yanbo , Socciarelli Fabio , Berkovska Olena , Umer Husen M. , Mermelekas Georgios , Pirmoradian Mohammad , Jönsson Mats , Brunnström Hans , Brustugun Odd Terje , Purohit Krishna Pinganksha , Cunningham Richard , Asl Hassan Foroughi , Isaksson Sofi , Arbajian Elsa , Aine Mattias , Karlsson Anna , Kotevska Marija , Hanson Carsten Gram , Haakensen Vilde Drageset , Helland Åslaug , Tamborero David , Johansson Henrik J. , Branca Rui M. , Planck Maria , Staaf Johan , Orre Lukas M. 
Published: November 2021

Abstract
The associated publication reports proteogenomic analysis of non-small cell lung cancer (NSCLC), where we identified molecular subtypes with distinct immune evasion mechanisms and therapeutic targets, and validated our classification method in separate clinical cohorts. This protocol describes sections of the bioinformatics analysis of the multi-omics data, namely, data analysis and processing for panel sequencing, identification of cancer- and driver-related proteins in proteomics data, proteogenomics search, and machine learning-based classifiers for NSCLC subtyping. Specifically, a cohort classifier was built using support-vector machine-recursive feature elimination (SVM-RFE) algorithm applied to in-depth proteomics data from a cohort of 141 samples. The classifier was then validated in three external datasets. Another classifier, suitable for single-sample subtyping, was built using k-top scoring pairs (k-TSP) algorithm applied to label-free data from a cohort of 136 samples. The k-TSP-based classifier was validated in two independent cohorts and an additional external dataset.
Lifestyle Medicine Strategies for Combating Sleepiness and Fatigue in Professional Drivers - The "Highway 2 Health" study
Authors: Ntoumas Ilias , Karatzaferi Christina , Dardiotis Efthimios , Sakkas Giorgos K. 
Published: November 2021

Abstract
Sleep has a number of health benefits, including memory and learning, vitality and energy as well as high quality of life levels. Lack of sleep impairs judgment, impacts longevity and safety, and increases the risk of a number of diseases including obesity, hypertension, heart disease, diabetes, mood disorders, and impaired immune function. In addition lack of sleep or disturbance of normal sleep cycle could have a major impact on people's lives and working performance such as driving. Daily sleepiness is a problem concerning professions with a non-fixed schedule. Specifically, professional long-haul drivers confront sleepiness problems and in combination with fatigue, they are prone to driving accidents and other incidents. Sleep quality and quantity are closed related to fatigue which is one of the most common reasons for driving and working accidents. In recent years, car accidents involving professional drives have increased significantly. The main reasons for those accidents were fatigue and sleepiness due to long hours of driving or difficult working conditions according to the recent European report (Driver Fatigue in European Road Transport - etf-europe.org).Lifestyle Medicine addresses health risk factors in primary, secondary, and tertiary prevention of developing disease rather than on acute care and reacting to illness, injury, and disease. Lifestyle Medicine strategies target modifiable risk factors, such as diet, sleep, stress, and physical activity. By applying those regimes the investigators could improve physical and mental health levels that can affect the quality of sleep, reducing daily sleepiness and fatigue, in professional drivers operating coaches and trucks. Any intervention that could improve alertness and reduce fatigue and sleepiness in those people, will automatically improve safety, reduce driving accidents and save lives and resources.The study is registered on the ClinicalTrials.gov public website (NCT05096130).
Assessment of The Renewable Energy Offshore and Wind Energy Markets Affects During The COVID-19 Virus
Authors: Jamali Riaz Hussain , Bughio Amanullah , Das Ghansham , Jamali Rizwan , Niroula Shova , Baloch Zulfiqar Ali 
Published: November 2021

Abstract
The main aims of the current study are to determine during the COVID-19 epidemic effects on renewable and green energy and control the raising the cost of utilizing renewable energy to power for country energy plants using the Levelized Cost of Energy methods. Objective 1) The COVID-19 pandemic can be provided through green financial policies such as coal pricing, transferable green certificates, and loans for renewable energy markets. Objective 2) examined the cost of offshore wind power in china before and after the COVID-19 outbreak, using data from 100 offshore wind power projects constructed between 2000 and 2020. Based on results, wind power's fixed average cost of electricity fell from 0.98 Chinese yuan in 2000 to 0.79 Chinese RMB in March 2019, and subsequently to 0.75 Chinese RMB in 2020, a 13.99 percent increase. Other results average electricity generation price down to 0.79 RMB, 0.99 RMB, and 0.79 RMB and average carbon oxide emissions was 50 RMB/ton increase. The green fiscal policies will be required during the COVID-19 epidemic to promote offshore wind energy generation investment.