bluuebunny/crossref_metadata_2025_split huggingface dataset:
Expression, Prognosis, and Regulation of ULBP1, ULBP2, and ULBP3 in Human Breast Cancer
Authors: Zhang Yutong , Han Caixia , Shao Enze , Sun Litao , Liu Dongzhe 
Published: June 2021

Abstract
The expression of NKG2D ligands (NKG2DLs), induced by stress or malignant transformation, is considered to mark dysfunctional cells for elimination by NK cells or cytotoxic lymphocytes via NKG2D/NKG2DLs pathway. ULBP1, ULBP2, and ULBP3 (ULBP1-3), three members of NKG2DLs, are common expressed in breast cancer. We analyzed the expression of ULBP1-3 in breast cancer and healthy control tissues with several databases, and found that breast cancer had a higher mRNA level of ULBP1 and ULBP2, and a higher protein level of ULBP1-3. Analysis with the bc-GenExMiner database showed that the expression of ULBP1-3 were down-regulated by the wild type P53, PR, and HER2+ in breast cancer. Except for ULBP1, ULBP2 and ULBP3 were associated with poor prognosis in breast cancer. The analysis of the correlated genes suggested that ULBP1-3 have some common pathways including NK cell-mediated cytotoxicity, microRNA in cancer, and IL-17 signaling pathway, whereas they also have their own pathways. But ULBP1-3 expression were also a significantly negative correlation with few immune markers on NK and central memory CD8+ T. The correlations and co-occurrence between ULBP1-3 and the other ligands by using TIMER and cbioportal databases showed the same form proteins or the proteins in the same family were more likely to change at the same time. Together with all these findings, increased ULBP2 and ULBP3 were correlated with poor prognosis and various markers on immune cells. These conclusions indicated that ULBP2 and ULBP3 could serve as potential biomarkers to assess prognosis and they were strong correlation with various markers in immune cells.
Thermoluminescence Properties of Bioglass for Radiation Dosimetry
Authors: Alazab Huda A. , Abdou N.Y. , Saudi H. A. , Abd-Allah wesam 
Published: June 2021

Abstract
The thermoluminescence technique was employed to study bioglass matrices prepared using the traditional technique of glass making. The synthesized bioglass matrices were investigated using X-ray diffraction (XRD), and differential thermal analysis (DTA) has been studied. The highest thermoluminescent intensity was found for the bioglass matric of 26.91% CaO, 46.134% SiO2, 2.60% P2O5, 24.34%Na2O (mol%), with only one glow peak at 460 k. The TL response illustrations linearity in high gamma dose range from 25 to 1000 Gy. This new bioglass matric might become useful in high-dose fields for dosimetry.
Copper Oxide Nanoparticles and Bulk Particles Stress Induced Cytological and Physiological Responses of Vicia Faba L.
Authors: Kumar Vaibhav , Khan Adiba , Srivast Alka , Verma Praveen Chandra , Saxena Gauri 
Published: June 2021

Abstract
CuO nanoparticles (NPs) and their bulk counter parts are being utilized in various industrial preparations. The progressive increase in the use of CuO NPs and bulk particles (BPs) eventually ends up in the environment, causing potential hazard to biota and imbalance in the abiotic components. In order to elucidate the toxic impact of CuO NPs and BPs, plant seedlings of Vicia faba var. Pusa Sumit were exposed to 20-100 mg L⁻1 of CuO NPs and BPs along with a control set up. Root tips and leaf tissues of plant seedlings were used to perform genotoxic and biochemical assays, respectively. Cytological preparations were used to screen mitotic indices (MI), micronuclei and chromosomal abnormalities (CAs). CuO NPs treatment led to 24.1 % reduction in MI and 7.9 % increase in CAs while BPs treatment reduced MI by 12.7 % and raised CAs by 4.3 % only. Bio-uptake of CuO NPs and BPs in the plant tissues is the key cause of oxidative stress. It triggered significant changes in lipid peroxidation and other biochemical parameters including enzymatic (peroxidase, superoxide dismutase, catalase, glutathione s-transferase and glutathione reductase) and non-enzymatic (photosynthetic pigments and proline content) components of antioxidant system in treated plant seedlings. In this study, CuO NPs caused 49.1 % to 96.7 % enhanced activity of antioxidant enzymes as compared to BPs. These findings revealed that CuO NPs were more toxic to plants than their counter BPs.
A Self-Attention Mechanism Neural Network for Detection and Diagnosis of COVID-19 from Chest X-Ray Images
Authors: Cheng Bo , Xiang Wei , Xue Ruhui , Yang Hang , Zhu Laili 
Published: June 2021

Abstract
The new type of coronavirus is called COVID-19. The virus can cause respiratory diseases, accompanied by cough, fever, difficulty breathing, and in severe cases, it can also cause symptoms such as pneumonia. It began to spread at the end of 2019 and has now spread to all parts of the world. The limited test kits and increasing number of cases encourage us to propose a deep learning model that can help radiologists and clinicians use chest X-rays to detect COVID-19 cases and show the diagnostic features of pneumonia. In this study, our methods are: 1) Propose a data enhancement method to increase the diversity of the data set, thereby improving the generalization performance of the network. 2) Using the deep convolutional neural network model DPN-SE, an attention mechanism is added on the basis of the DPN network, which greatly improves the performance of the network. 3) Use the lime interpretable library to mark the X-ray, the characteristic area on the medical image that is helpful for the doctor to make a diagnosis. The model we proposed can obtain better results with the least amount of data preprocessing given limited data. In general, the proposed method and model can effectively become a very useful tool for clinical practitioners and radiologists.
Polymorphism and Distribution of Ace Gene Involved in the Resistance of Musca Domestica to Organophosphates in Guizhou Province of China
Authors: Mou Rong , Yang Xi , Liang Qiuguo , Cheng Jinzhi , Wu Yuanming , Wang Gang , Tan Weilong , Wu Jia Hong 
Published: June 2021

Abstract
Acetylcholinesterase is the primary target of organophosphates (OPs) and carbamates in insects. As gene mutation has been verified as an important mechanism of insecticide resistance in insects, in this study, we investigated the status of OPs resistance and the polymorphism of ace gene (that encodes acetylcholinesterase) in housefly (Musca domestica L) field populations in Guizhou Province, China. Bioassays showed that the houseflies had 142.16–303.54-fold resistance to dichlorvos (DDVP) and 122.13–363.98-fold resistance to temephos. Molecular analysis revealed resistance-causing mutations of ace gene at loci of 260, 342 and 407 in the housefly populations, with a total frequency of 55%, 100% and 94%, respectively. In addition, 11 combinations of ace mutation were observed across the detected populations. The most frequently detected combination was L/V+A/V+Y, followed by L+A+Y and L/V+A+Y. No significant relationship was found between single mutation/combination mutations and DDVP resistance. These results indicate that the OPs resistance is prevalent among the housefly populations in Guizhou Province, with a similar pattern of allele mutation of ace across China. The target resistance can not fully account for the resistance of houseflies to OPs in Guizhou.
Pyrolysis and Kinetic Analysis of CO2 Fixation Marine (Isochrysis sp.) and Freshwater (Monoraphidium c.) Microalgae
Authors: Osman Noridah B. , Amina Umi Syahirah Binti Mohd , Patrick David Onoja , Zamana Nurul Asyikin Binti Bad ir Noon , Saado Syazmi Zul Arif n Hakimi , Yusup Suzana , Chik Muhammad Nazry , Yahya Liyana 
Published: June 2021

Abstract
Marine and freshwater microalgae grow in two different ecosystems, which influence their properties thus requires attention prior to determining its application. This paper has successfully disclosed the thermal, chemical, and physical properties of two types of microalgae on carbon dioxide (CO2) fixation and underwent pyrolysis process. Slow pyrolysis process for marine and freshwater microalgae (Isochrysis sp. and Monoraphidium c.) was performed in the fixed bed pyrolysis reactor and TGA (thermogravimetric analyzer) to determine the product yield and study their thermal decomposition profile. The pyrolysis was completed at various temperatures (400, 450, 500, and 550°C) at a heating rate of 15 °Cmin-1 and nitrogen flow rate of 200 ml min-1. Pyrolysis in TGA analyzer ran from 27 to 800°C at three heating rates (10, 20, and 40 °Cmin-1). For chemical composition, Fourier-transform Infrared (FTIR) analysis was performed on both microalgae samples. The highest yield (up to 33.9%) of bio-oil was obtained from Isochrysis sp. for all temperatures while the highest average yield (65.78%) of bio-char was collected from Monoraphidium c. species. From TGA pyrolysis, the major decomposition occurred between 200-400°C for Monoraphidium c. species. On the other hand, the decomposition profile of Isochrysis sp. was slightly slower, which may be due to the differences in lipid composition (FTIR peak 2929 cm-1). The activation energy of all tests is lower (33.6-40.3 kJ mol-1) compared to several other biomasses. Marine species fixed with CO2 showed promising results even without addition of catalyst and no additional cost needed.
The Effect of using Repetitively Pulsed Laser Radiation in Selective Laser Melting when Creating a Metal Matrix Composite Ti-6Al-4V – B4C.
Authors: Golyshev Alexander , Malikov Alexander , Orishich Anatoliy , Gulov Mikhail , Ancharov Alexei 
Published: June 2021

Abstract
A metal-matrix composite based on Ti-6Al-4V – B4C with TiB, TiB2 and TiC inclusions was successfully obtained as a result of in-situ synthesis using repetitively pulsed laser radiation. For the first time, the phase composition of the obtained metal-matrix composite was studied using synchrotron radiation. A comparison of the effect of using continuous and pulsed-periodic radiation in selective laser melting on the microstructure and mechanical properties of coatings was made. The use of repetitively pulsed radiation made it possible to form more uniform structures and to improve the mechanical properties of metal-matrix coatings in comparison with the continuous mode of exposure. It has been established that the use of repetitively pulsed radiation and the formation of TiB2, TiB, TiC phases made it possible to increase the wear resistance of the formed composite by a factor of 6 in comparison with the Ti-6Al-4V metal coating.
Green Construction Practices: Ensuring Client Satisfaction Through Health and Safety Performance
Authors: Onubi Hilary Omatule , Yusof Nor'Aini , Hassan Ahmad Sanusi 
Published: June 2021

Abstract
Studies have proved that Client Satisfaction (CS) is greatly affected by the nature of construction practices carried out on site. However, not much attention has been given to CS in projects that adopt green construction practices (GCPs). This study examines the effect of GCPs on CS, and the means by which CS can be achieved through health and safety performance (HSP). This study also analyses how the relationship between HSP and CS could vary depending on the level of GCPs adopted. A questionnaire with 27 items drawn from literature was used in collecting data from class A contractors in Nigeria. The study’s hypotheses were tested using the Partial Least Squares Structural Equation Modeling (PLS-SEM) technique. The findings of the study reveal that GCPs has a positive effect on CS. HSP proved to only partially mediate the relationship between GCPs and CS. The findings also show that the effect of HSP on CS is moderated by GCPs with the stronger effect from projects with low adoption of GCPs. The study findings highlight the pivotal role of HSP in ensuring CS in construction projects that adopt GCPs while also making theoretical and practical contributions to guide policy makers, scholars, and contractors.
Clinical Application of One-Position Complete Retroperitoneoscopic Nephroureterectomy
Authors: Xiao Yongshuang , Chen Tan , Li Qinghui , Wen Rumin , Li Shuofeng 
Published: June 2021

Abstract
Objective: To explore the surgical method and the clinical efficacy of complete retroperitoneal laparoscopic radical resection of upper tract urothelial carcinoma (UTUC). Methods: A retrospective analysis of 83 patients with upper urothelial carcinoma from January 2016 to December 2019 in the Affiliated Hospital of Xuzhou Medical University was conducted. Among them, 40 patients underwent one-position complete retroperitoneoscopic nephroureterectomy (CRNU) and 43 patients underwent traditional retroperitoneoscopic nephroureterectomy (TRNU). Compare the differences in clinical data, perioperative parameters and postoperative follow-up results of patients with the two surgical methods. CTU and ureteroscopy were used to diagnose. Results: A total of 43 patients (35 males and 8 females) with TRNU were enrolled in this study. Forty patients (31 males and 9 females) received CRNU. The CRNU group had significantly shorter average operation time (105.83±5.80 min versus 147.28 ± 17.58 min) and lower visual pain score (P=0,024). No significant difference was found in age, BMI, T stage, complication, change of albumin and hemoglobin, postoperative hospital stays and tumor recurrence (P>0.05). Conclusion: one-position complete retroperitoneoscopic nephroureterectomy is safe and feasible. The curative effect is affirmative. It has the conspicuous advantages in minimally invasive, no posture change, less postoperative pain, less interference with abdominal organ and shorter operation time, which could particularly reduce the workload of operating room nurses without any additional postoperative complications.
Bowl Surface Laser Forming Process of Stainless Steel Composite Plate
Authors: Wang Xiaogang , Shi Yongjun , Guo Yankuo , Wang Qin 
Published: June 2021

Abstract
In this paper, the three-dimensional bowl surface was obtained by reasonably planning the radial heating path by taking the laser as the heat source and the stainless steel composite plate (06Cr19Ni10/1Cr17Mn6Ni5N/06Cr19Ni10) as the research object, and the forming process of the three-dimensional bowl surface was studied. It was found that the target surface bending forming obtained by inside-outside symmetric scanning strategy (Strategy d) had the highest accuracy. The plate deformation increased with the increase of laser power (P), increased then decreased with the increase of heating line length (L), and decreased with the increase of scanning speed (V). Moreover, the deformation was approximately linear with the number of repeated heating (N), with the optimal process conditions of P = 500W, V = 10 mm/s, L = 20 mm, N = 7. A three-dimensional thermodynamic coupling model of a three-layer composite plate was established and verified experimentally.
Identification of LOXL3-Associating Immune Infiltration Landscape and Prognostic Value in Hepatocellular Carcinoma
Authors: Wang Ning , Zhou Xue , Tang Fei , Wang Xue , Zhu Xiaowei 
Published: June 2021

Abstract
Hepatocellular carcinoma (HCC), the most common type of hepatic malignancies, remains a global health challenge with multiple aetiologies and low five-year survival rate. In recent years, breakthroughs in the field of tumor immunotherapy with immune checkpoint inhibitors (ICIs) have made a therapeutic revolution, which has been shown to improve the prognosis of patients with HCC. Immune infiltrates represent a major component of tumor microenvironment (TME), and play an essential role in both tumor progression and therapeutic response. The major unmet challenge in tumor immunotherapy is exploring the intrinsic and extrinsic mechanisms of TME promoting the management of HCC. Lysyl oxidase like 3 (LOXL3) participates in the remodeling of extracellular matrix (ECM) and the cross-linking of collagen and elastic fibers. It has been reported that LOXL3 is associated with the development and tumorigenesis of multiple types of cancer. In this study, we first found that LOXL3 gene was upregulated in tumor tissues compared with the normal tissues. Furthermore, LOXL3 expression is positively correlated with the infiltration of multiple immune cells and the expression of immune checkpoint genes in HCC. Meanwhile, high LOXL3 expression predicted poor outcomes of the patients with HCC. Functional enrichment analysis suggested that LOXL3 was mainly linked to extracellular structure and matrix organization, cell−cell adhesion, and T cell activation. This is the first comprehensive study to indicate that LOXL3 is correlated with immune infiltrates and may serve as a novel biomarker predicting prognosis and immunotherapy in HCC.
Isolation, Identification and Genetic Characteristics of an Uncommon Norovirus Genotype, GIX.1[GII.P15], From Kunming, China
Authors: Chen Yanli , Wu Qiongwen , Li Guiman , Li Hongzhe , Li Wenlong , Qin Li , Zheng Huiwen , Liu Changkun , Li Heng , Hou Min , Liu Longding 
Published: June 2021

Abstract
Human norovirus, an RNA virus of the family Caliciviridae, is a common viral pathogen causing acute gastroenteritis of all age groups worldwide. To date, tens of thousands genome sequences of norovirus have been uploaded to NCBI database, more than half of them were epidemic strains of GII.4 or GII.17 genotype. However, sequence information on the non-epidemic norovirus strains remains poorly studied. In this study, an uncommon norovirus genotype, GIX.1[GII.P15], was isolated using Raji cells and the full-genome sequence of the strain was extensively characterized. The norovirus virus particles with a diameter of approximately 30 nm and a morphology of spherical and lace-like appearance were observed by electron microscopy. Viral genome replication in Raji cells were confirmed by real-time quantitative reverse transcription-PCR from viral replication kinetics and passaging experiments of the primary virus. Phylogenetic analysis showed that the strain (KMN1) belonged to the GIX.1[GII.P15] genotype and indicated that no recombination has occurred in this strain thus far. Further compared analysis of the full genome sequence with the consensus sequence of GIX.1[GII.P15] genomes revealed a total of 81 nucleotide substitutions (53 in ORF1, 20 in ORF2, and 8 in ORF3) across the genome, but only 6 substitutions resulted in amino acid changes (3 in ORF1, 1 in ORF2, and 2 in ORF3). Moreover, one amino acid substitution at the 302 amino acid site (P302S) was observed in the P2 domain of the capsid protein, and the site was around one of the predicted conformational epitopes on the VP1 protein structure. The genomic information obtained from the novel strain may extend the understanding of the non-epidemic GIX.1[GII.P15] strains.
Ceftizoxime Loaded ZnO/L-Cysteine Based an Advanced Nanocarrier Drug for Growth Inhibition of Salmonella Typhimurium
Authors: Bacchu Md. Sadek , Ali Md. Romzan , Setu Md. Ali Ahasan , Akter Selina , Khan Md. Zaved Hossain 
Published: June 2021

Abstract
L-Cysteine coated zinc oxide (ZnO) nano hollow spheres were prepared as a potent drug delivery agent to eradicate Salmonella enterica serovar Typhimurium (S. typhimurium). The ZnO nano hollow spheres were synthesized by following the environmentally-friendly trisodium citrate assisted method and L-Cysteine (L-Cys) conjugate with its surface. ZnO/L-Cys@CFX nanocarrier drug has been fabricated by incorporating ceftizoxime with L-Cys coated ZnO nano hollow spheres and characterized using different techniques such as scanning electron microscope (SEM), attenuated total reflection Fourier transform infrared (ATR-FTIR), and X-ray diffraction (XRD) etc. Furthermore, the drug-loading and encapsulation efficiency at different pH levels was measured using UV-vis spectrometer and optimized. A control and gradual manner of pH-sensitive release profile was found after investigating the release profile of CFX from the carrier drug. The antibacterial activity of ZnO/L-Cys@CFX and CFX were evaluated through the agar disc diffusion method and the broth dilution method, which indicate the antibacterial properties of antibiotics enhance after conjugating. Surprisingly, the ZnO/L-Cys@CFX exhibits a minimum inhibitory concentration (MIC) of 5µg/ml against S. typhimurium is lower than CFX (20µg/ml) itself. These results indicate the nanocarrier can reduce the amount of CFX dosed to eradicate S. typhimurium.
Evaluation of Alveolar Bone Hypomineralization in Hypophosphatasia Patients Using Orthopantomography
Authors: Okawa Rena , Nakamoto Takashi , Matayoshi Saaya , Nakano Kazuhiko , Kakimoto Naoya 
Published: June 2021

Abstract
Hypophosphatasia (HPP) is a metabolic disease characterized by impaired bone mineralization and early exfoliation of primary teeth. The purpose of this study was to develop a method for quantitatively evaluating alveolar bone hypomineralization using orthopantomographic images. Alveolar bone density was defined according to the pixel values and corrected by brightness shown by an indicator applied to the orthopantomographic device. Images of 200 systemically healthy subjects (aged 2–15 years) were classified into five age groups. The corrected pixel values were significantly lower in the younger group than in those aged 14–15 years (2–4, 5–7 and 8–10 years versus 14–15 years: P < 0.0001, 11–13 years versus 14–15 years: P < 0.01). Orthopantomographic images of 17 HPP patients were evaluated. The corrected pixel values of three-quarters of the odonto type HPP patients were lower than the mean values for the healthy group. One-third of patients treated with enzyme replacement therapy showed higher corrected pixel values than the healthy group. Our results suggest that odonto type HPP without skeletal problems is occasionally accompanied by hypomineralization of alveolar bone, and that alveolar bone hypomineralization in severe HPP patients is possibly improved by enzyme replacement therapy.
Tylosin Inhibited Growth of Chlorella Vulgaris and Raphidocelis Subcapitata by Inducing Oxidative Stress
Authors: Lu Denglong , Ma Zhihua , Peng Jianglin , Zhang Yibo , Liu Shan , Li Qi 
Published: June 2021

Abstract
Two model algae Chlorella vulgaris (C. vulgaris) and Raphidocelis subcapitata (R. subcapitata) were generally used to test chemicals with antimicrobial properties during registration process. However, it has been reported that significant sensitivity difference in two algae when exposure to antibiotics. Furthermore, the selection of an appropriate test species play a vital role in evaluate of environmental hazards and risks of compounds. Since the balance between oxidative stress and antioxidant is a crucial factor on alga growth. This experiment is performed to investigate the working of oxidative stress and mechanism of antioxidant defense system of algae under antibiotic stress. A series of concentration of Tylosin (TYN), a macrolide antibiotic, were used to test in this study. Oxidative stress biomarkers (Malondialdehyde (MDA)), non-enzymatic antioxidants (Reduced glutathione (GSH)), antioxidant enzymes (Superoxide dismutase (SOD), Catalase (CAT), Glutathione Peroxidase (GP), Glutathione S-transferase (GST)) and photosynthetic pigments were measured to investigate antioxidant defense system. R. subcapitata was significantly inhibited with increasing concentration of TYN, whereas no effects on C. vulgaris. The contents of MDA increased significantly when species were inhibited, and thus, activating the antioxidant system, companying with the significantly increasing of SOD and CAT.