bluuebunny/crossref_metadata_2025_split huggingface dataset:
Sex-Specific Difference in Dynamic Balance Following Total Hip Replacement
Authors: Queen Robin M , Schmitt Daniel 
Published: June 2021

Abstract

Background and Objectives
Total hip arthroplasty (THA) is a common surgical procedure in older adults (65 years or older). THA has high patient satisfaction, but little is known about balance and mobility limitations after surgery and if outcomes are sex-specific. This study was aimed to evaluate post-THA asymmetry during unilateral standing and a dynamic balance and reach test and test the hypotheses that balance performance would be decreased on the surgical limb and that balance deficits would be greater in women than in men.

Research Design and Methods
Primary, unilateral THA (70 male, 57 female) patients completed a bilateral 10-s single-leg stance test. Sixty male but only 34 female participants could maintain unilateral balance for 10 s or greater. The cohort who successfully completed the 10-s single-limb stance test then completed a Lower Quarter Y-Balance Test in which the maximum anterior (ANT), posteromedial (PM), and posterolateral reach distances were obtained bilaterally and used to calculate the asymmetry score. All variables were compared using a mixed-model repeated-measures analysis of variance (sex by limb), while independent samples t tests were used to assess sex-specific asymmetry.

Results
Women failed single-leg stance at a higher rate than men (85.7% vs 59.6%; p = .001). Reach distance was different between limbs for all reach directions (p &lt; .004) with greater reach distance on the nonoperative limb for all patients. Men had a greater reach distance in the ANT (p = .004) and PM (p = .006) directions.

Discussion and Implications
These results indicate that post-THA, the operative limb and female patients have greater balance limitations. These results are novel and reveal sex-specific patterns that emphasize the need for sex-specific postoperative rehabilitation programs to improve long-term outcomes, especially in older adults with muscle weakness and balance deficits.
Maximum covariance direction method for unconventional seismic sources
Authors: Zhu Juan , Chen Xiaohan , Wen Lianxing 
Published: June 2021

SUMMARYWe propose a new array covariance matrix analysis method, named ‘maximum covariance direction method’ (MCD method), to detect and locate unconventional seismic sources of weak signals without clear onsets. The MCD method builds a normalized-covariance matrix of time-shifted seismic waveforms recorded in a seismic array and determines the existence of source based on the consistency of the maximum covariance direction with the theoretical prediction. Synthetic tests demonstrate effectiveness of the MCD method in detecting one and multiple isolated sources with low signal-to-noise ratios. As a data application, we study 1-hr long-period tremors (LPTs) around Aso Volcano of Japan in 2014 November 24. A total of 26 LPTs are detected near the Naka-dake first crater of Aso Volcano, with the uncertainties of source location of about 7 km. Using the recorded background noise at the seismic stations, we show that the MCD method can detect LPTs even when the LPT signals are buried in the background noise and become indiscernible in the seismic data. Unlike traditional methods that employ the coherent features of seismic signals for source detection, the MCD method places emphases on both the coherence of seismic signals and consistency of the direction of the coherent signals from a potential source location. The synthetic tests and data application indicate that the MCD method provides a good alternative to other traditional methods for detecting and locating unconventional seismic sources, with a major improvement of avoiding source misidentification in the presence of strong incoherent signals.
Novel xylan degrading enzymes from polysaccharide utilizing loci of Prevotella copri DSM18205
Authors: Linares-Pastén Javier A , Hero Johan Sebastian , Pisa José Horacio , Teixeira Cristina , Nyman Margareta , Adlercreutz Patrick , Martinez M Alejandra , Karlsson Eva Nordberg 
Published: June 2021

Abstract
Prevotella copri is a bacterium that can be found in the human gastrointestinal tract. The role of P. copri in the GIT is unclear, and elevated numbers of the microbe have been reported both in dietary fiber-induced improvement in glucose metabolism but also in conjunction with certain inflammatory conditions. These findings raised our interest in investigating the possibility of P. copri to grow on xylan, and identify the enzyme systems playing a role in digestion of xylan-based dietary fibers. Two xylan degrading polysaccharide utilizing loci (PUL10 and 15) were found in the genome, with three and eight glycoside hydrolase (GH) -encoding genes, respectively. Three of them were successfully produced in Escherichia coli: One extracellular enzyme from GH43 (subfamily 12, in PUL10, 60 kDa) and two enzymes from PUL15, one extracellular GH10 (41 kDa), and one intracellular GH43 (subfamily 137 kDa). Based on our results we propose that in PUL15, GH10 (1) is an extracellular endo-1,4-β-xylanase, that hydrolyses mainly glucuronosylated xylan polymers to xylooligosaccharides (XOS); while, GH43_1 in the same PUL, is an intracellular β-xylosidase, catalysing complete hydrolysis of the XOS to xylose. In PUL10, the characterized GH43_12 is an arabinofuranosidase, with a role in degradation of arabinoxylan, catalysing removal of arabinose-residues on xylan.
Sexual Selection and Introgression in Avian Hybrid Zones: Spotlight onManacus
Authors: Bennett Kevin F P , Lim Haw Chuan , Braun Michael J 
Published: June 2021

AbstractHybrid zones offer a window into the processes and outcomes of evolution, from species formation or fusion to genomic underpinnings of specific traits and isolating mechanisms. Sexual selection is believed to be an important factor in speciation processes, and hybrid zones present special opportunities to probe its impact. The manakins (Aves, Pipridae) are a promising group in which to study the interplay of sexual selection and natural hybridization: they show substantial variation across the family in the strength of sexual selection they experience, they readily hybridize within and between genera, and they appear to have formed hybrid species, a rare event in birds. A hybrid zone between two manakins in the genus Manacus is unusual in that plumage and behavioral traits of one species have introgressed asymmetrically into populations of the second species through positive sexual selection, then apparently stalled at a river barrier. This is one of a handful of documented examples of asymmetric sexual trait introgression with a known selective mechanism. It offers opportunities to examine reproductive isolation, introgression, plumage color evolution, and natural factors enhancing or constraining the effects of sexual selection in real time. Here, we review previous work in this system, propose new hypotheses for observed patterns, and recommend approaches to test them.
All the World’s a Stage: US Presidential Narcissism and International Conflict
Authors: Harden John P 
Published: June 2021

AbstractHow do leaders matter? What do leaders want? Grandiose narcissism provides a pathway to understanding how personality can impact a leader’s preference formation and foreign policy behavior. More narcissistic leaders will focus their efforts on maintaining their inflated self-image by selecting how they will fight on the world stage and who they will fight against. While most leaders will divert attention to easier won battles, more narcissistic leaders will prefer to fight against high-status states by themselves. This article introduces a new measure of US’ presidential narcissism, and finds support for the argument that more narcissistic US presidents prefer unilaterally initiating Great Power disputes using data from 1897–2008. A brief review of Theodore Roosevelt’s handling of the Venezuela Crisis of 1902–1903 is used as a plausibility probe of the theory’s causal mechanisms.
Sensitive Screening of New Psychoactive Substances in Serum Using Liquid Chromatography–Quadrupole Time-of-Flight Mass Spectrometry
Authors: Kleis Jan-Niklas , Hess Cornelius , Germerott Tanja , Roehrich Joerg 
Published: June 2021

Abstract
Analysis of new psychoactive substances (NPS) still poses a challenge for many institutions due to the number of available substances and the constantly changing drug market. Both new and well-known substances keep appearing and disappearing on the market, making it hard to adapt analytical methods in a timely manner. In this study we developed a qualitative screening approach for serum samples by means of liquid chromatography--quadrupole time-of-flight mass spectrometry. Samples were measured in data-dependent auto tandem mass spectrometry mode and identified by fragment spectra comparison, retention time and accurate mass. Approximately 500 NPS, including 195 synthetic cannabinoids, 180 stimulants, 86 hallucinogens, 26 benzodiazepines and 7 others were investigated. Serum samples were fortified to 1 ng/mL and 10 ng/mL concentrations to estimate approximate limits of identification (LOIs). Samples were extracted using solid-phase extraction with non-endcapped C18 material and elution in two consecutive steps. Benzodiazepines were eluted in the first step, while substances of other NPS subclasses were distributed among both extracts. To determine LOIs, both extracts were combined. Ninety-six percent (470/492) of investigated NPS were detected in 10 ng/mL samples and 88% (432/492) were detected in 1 ng/mL samples. Stimulants stood out with higher LOIs, possibly due to instability of certain methcathinone derivatives. However, considering relevant blood concentrations, the method provided sufficient sensitivity for stimulants as well as other NPS subclasses. Data-dependent acquisition was proven to provide high sensitivity and reliability when combined with an information-dependent preferred list, without losing its untargeted operation principle. Summarizing, the developed method fulfilled its purpose as a sensitive untargeted screening for serum samples and allows uncomplicated expansion of the spectral library to include thousands of targets.
Brassinolide inhibits flavonoid biosynthesis and red-flesh coloration via the MdBEH2.2–MdMYB60 complex in apple
Authors: Wang Yicheng , Mao Zuolin , Jiang Huiyan , Zhang Zongying , Wang Nan , Chen Xuesen 
Published: June 2021

AbstractFlavonoid content, which is an important indicator of the nutritional value of fruits and vegetables, directly determines the marketability of many fruit crops, including apple (Malus domestica). Brassinosteroids (BRs) are steroid hormones that affect flavonoid biosynthesis in plants, but the underlying regulatory mechanism remains unclear. In this study, treatments with brassinolide (the most active BR) and brassinazole (a BR biosynthesis inhibitor) decreased and increased, respectively, the flavonoid, anthocyanin, and proanthocyanidin (PA) content in red-fleshed apple seedlings and calli. We subsequently demonstrated that a BZR (BRI1-EMS-suppressor (BES)/brassinazole-resistant) family transcription factor, MdBEH2.2, participates in BR-regulated flavonoid biosynthesis. Specifically, MdBEH2.2 inhibits the accumulation of flavonoids, anthocyanins, and PAs in apple seedlings; however, brassinazole treatment weakens the inhibitory effect. Additionally, we confirmed that a BR-induced MYB TF, MdMYB60, interacts with MdBEH2.2. The resulting MdBEH2.2–MdMYB60 complex further enhances the inhibitory effect of MdBEH2.2 or MdMYB60 on the transcription of flavonoid biosynthesis-related genes. These results indicate that brassinolide decreases flavonoid content through the MdBEH2.2–MdMYB60 regulatory module. Our findings further clarify the molecular mechanism mediating the regulation of flavonoid biosynthesis by BR signals in horticultural crops.
Multiomics analysis of kernel development in response to short-term heat stress at the grain formation stage in waxy maize
Authors: Guo Jian , Gu Xiaotian , Lu Weiping , Lu Dalei 
Published: June 2021

Abstract
Understanding the adaptive changes in maize kernels under high-temperature stress during grain formation stage is critical for developing strategies to alleviate the negative effects on yield and quality. In this study, we subjected waxy maize (Zea mays L. sinensis Kulesh) to four different temperature regimes from 1–15 d after pollination (DAP), namely normal day/normal night (control), hot day/normal night, normal day/hot night, and hot day/hot night. Compared to the control, the three high-temperature treatments inhibited kernel development and starch deposition. To understand how the kernels responded to high-temperature stress, their transcriptomes, proteomes, and metabolomes were studied at 10 DAP and 25 DAP. This showed that genes and proteins related to kernel development and starch deposition were up- and down-regulated, respectively, at 10 DAP, but this pattern was reversed at 25 DAP. Metabolome profiling under high-temperature stress showed that the accumulation patterns of metabolites at 10 DAP and 25 DAP were inversely related. Our multiomics analyses indicated that the response to high-temperature stress of signaling pathways mediated by auxin, abscisic acid, and salicylic acid was more active at 10 DAP than at 25 DAP. These results confirmed that high-temperature stress during early kernel development has a carry-over effect on later development. Taken together, our multiomics profiles of developing kernels under high-temperature stress provide insights into the processes that underlie maize yield and quality under high-temperature conditions.
The CaM1-associated CCaMK–MKK1/6 cascade positively affects lateral root growth via auxin signaling under salt stress in rice
Authors: Yang Jun , Ji Lingxiao , Liu Shuang , Jing Pei , Hu Jin , Jin Deming , Wang Lingqiang , Xie Guosheng 
Published: June 2021

Abstract
Ca2+/calmodulin (CaM)-dependent protein kinases (CCaMKs) and mitogen-activated protein kinase kinases (MAPKKs) are two types of kinases that regulate salt stress response in plants. It remains unclear, however, how they cooperatively affect lateral root growth under salt stress. Here, two conserved phosphorylation sites (S102 and T118) of OsCaM1 were identified, and found to affect the ability to bind to Ca2+in vitro and the kinase activity of OsCCaMK in vivo. OsCCaMK specifically interacted with OsMKK1/6 in a Ca2+/CaM-dependent manner. In vitro kinase and in vivo dual-luciferase assays revealed that OsCCaMK phosphorylated OsMKK6 while OsMKK1 phosphorylated OsCCaMK. Overexpression and antisense-RNA repression expression of OsCaM1-1, and CRISPR/Cas9-mediated gene editing mutations of OsMKK1, OsMKK6, and OsMKK1/6 proved that OsCaM1-1, OsMKK1, and OsMKK6 enhanced the auxin content in roots and lateral root growth under salt stress. Consistently, OsCaM1-1, OsMKK1, and OsMKK6 regulated the transcript levels of the genes of this cascade, and salt stress-related and lateral root growth-related auxin signaling under salt stress in rice roots. These findings demonstrate that the OsCaM1-associated OsCCaMK–OsMKK1/6 cascade plays a critical role in recruiting auxin signaling in rice roots. These results also provide new insight into the regulatory mechanism of the CaM-mediated phosphorylation relay cascade to auxin signaling in lateral root growth under salt stress in plants.
Detailed characterization of the UMAMIT proteins provides insight into their evolution, amino acid transport properties, and role in the plant
Authors: Zhao Chengsong , Pratelli Réjane , Yu Shi , Shelley Brett , Collakova Eva , Pilot Guillaume 
Published: June 2021

Abstract
Amino acid transporters play a critical role in distributing amino acids within the cell compartments and between plant organs. Despite this importance, relatively few amino acid transporter genes have been characterized and their role elucidated with certainty. Two main families of proteins encode amino acid transporters in plants: the amino acid–polyamine–organocation superfamily, containing mostly importers, and the UMAMIT (usually multiple acids move in and out transporter) family, apparently encoding exporters, totaling 63 and 44 genes in Arabidopsis, respectively. Knowledge of UMAMITs is scarce, based on six Arabidopsis genes and a handful of genes from other species. To gain insight into the role of the members of this family and provide data to be used for future characterization, we studied the evolution of the UMAMITs in plants, and determined the functional properties, the structure, and localization of the 47 Arabidopsis UMAMITs. Our analysis showed that the AtUMAMITs are essentially localized at the tonoplast or the plasma membrane, and that most of them are able to export amino acids from the cytosol, confirming a role in intra- and intercellular amino acid transport. As an example, this set of data was used to hypothesize the role of a few AtUMAMITs in the plant and the cell.
Trimethylamine-N-Oxide and Precursors as Novel Potential Biomarkers for Anxiety Disorder
Authors: Le Juan , Peng Rui , Li Yan 
Published: June 2021

Abstract

Background
Anxiety disorder (AD) is closely related to changes in the composition of the gut microbiota, and changes in gut microbiota abundance affect the synthesis of trimethylamine N-oxide (TMAO).

Objective
To explore whether TMAO is related to and serves as a potential biomarker for the diagnosis of AD.

Methods
The concentrations of TMAO, choline, and betaine were analyzed in 60 patients with AD and 60 control individuals using a liquid chromatography–tandem mass spectrometry (LC-MS/MS) method.

Results
Compared with controls, TMAO was significantly reduced in patients with AD. Logistic regression analysis demonstrated that decreased TMAO concentrations were associated with an increased risk of AD. The multiplication of TMAO and its precursors (choline and betaine) produced the best AUC for the diagnosis of AD (AUC = 0.847; 95% CI, 0.780–0.914; P &lt;.001).

Conclusion
The decrease of TMAO concentration is related to the increase of anxiety disorder. TMAO and precursors could be identified as novel potential biomarkers for the diagnosis of AD.
Growing Better, Not Going Faster: World War I, Holy Land Mania, and Transnational Exchange in the Works of Abraham Mitrie Rihbany
Authors: Eltahawy Nora 
Published: June 2021

AbstractThis article analyzes the three works published by Arab American theologian and author Abraham Mitrie Rihbany during and in the aftermath of World War I: Militant America and Jesus Christ (1917), America Save the Near East (1918), and Wise Men from the East and from the West (1922). The political climate in which Rihbany wrote the works saw the American public grappling with two issues of particular relevance to the steadily growing Arab American community. Where the global front was concerned, debates on the merits of abandoning isolationist policies, which focused near exclusively on the situation in Europe, left Americans oblivious to the ongoing conflict between the Ottoman Empire and its Arab subjects. On the domestic front, rising levels of xenophobia and the lasting legacy of The Naturalization Act divided legal and public opinion on Arabs’ eligibility for citizenship. Situating Rihbany’s attempts to address both of these problems against the backdrop of his upbringing in Greater Syria, this article reveals how Rihbany called on his training in the cosmopolitan era of the Nahda in order to guide the American public toward a more expansive model of transnationalism capable of encompassing both Arabs and Arab Americans in its fold.
Mandatory HPV Vaccination; Opportunity to Save Lives, Improve Readiness and Cut Costs
Authors: Sitler Collin A , Weir Larissa F , Keyser Erin A , Casablanca Yovanni , Hope Erica 
Published: June 2021

ABSTRACT
Human papillomavirus (HPV) is the most common sexually transmitted infection (STI) in the U.S. military and accounts for more healthcare visits than the next two most common STIs combined. Human papillomavirus is preventable with a safe, effective, prophylactic vaccine that has been available since 2006, yet vaccination rates remain low. The vaccine is approved for females and males aged 9-45 years for prevention of HPV-related dysplasia and cancers. Although it is recommended by the Centers for Disease Control and Prevention (CDC)’s Advisory Committee on Immunization Practices (ACIP), it is not part of the U.S. military’s mandatory vaccine list. Human papillomavirus does not just affect female service members—male service members have a higher reported seropositive rate than their civilian counterparts and can develop oropharyngeal, anal, or penile cancers as sequelae of HPV. Oropharyngeal cancer, more common in males, is the fastest growing and most prevalent HPV-related cancer in the USA. Several countries, such as Australia and Sweden, have successfully implemented mandatory vaccine programs and have seen rates of HPV-related diseases, including cancer, decline significantly. Some models project that cervical cancer, which is the fifth-most common cancer in active duty women, will be eliminated in the next 20 years as a result of mandatory vaccination programs. Between higher seropositive rates and lack of widespread vaccination, HPV dysplasia and cancer result in lost work time, decreased force readiness, negative monetary implications, and even separation from service. With more than half of the 1.3 million service members in the catch-up vaccination age range of less than 26 years of age, we are poised to have a profound impact through mandatory active duty service member vaccination. Although multiple strategies for improving vaccination rates have been proposed, mandatory vaccination would be in line with current joint service policy that requires all ACIP-recommended vaccines. It is time to update the joint service guidelines and add HPV vaccine to the list of mandatory vaccines.
How much water was delivered from the asteroid belt to the Earth after its formation?
Authors: Martin Rebecca G , Livio Mario 
Published: June 2021

ABSTRACT
The Earth contains between 1 and 10 oceans of water, including water within the mantle, where 1 ocean is the mass of water on the Earth’s surface today. With N-body simulations, we consider how much water could have been delivered from the asteroid belt to the Earth after its formation. Asteroids are delivered from unstable regions near resonances with the giant planets. We compare the relative impact efficiencies from the ν6 resonance, the 2:1 mean motion resonance with Jupiter and the outer asteroid belt. The ν6 resonance provides the largest supply of asteroids to the Earth, with about $2{{\ \rm per\ cent}}$ of asteroids from that region colliding with the Earth. Asteroids located in mean motion resonances with Jupiter and in the outer asteroid belt have negligible Earth-collision probabilities. The maximum number of Earth collisions occurs if the asteroids in the primordial asteroid belt are first moved into the ν6 resonance location (through asteroid–asteroid interactions or otherwise) before their eccentricity is excited sufficiently for Earth collision. A maximum of about eight oceans of water may be delivered to the Earth. Thus, if the Earth contains 10 or more oceans of water, the Earth likely formed with a significant fraction of this water.