bluuebunny/crossref_metadata_2025_split huggingface dataset:
A minimal dose of unmanipulated bone marrow infusion without conditioning for T-B+NK- severe combined immunodeficiency
Authors: Yi Eun Sang , Ju Hee Young , Cho Hee Won , Lee Ji Won , Sung Ki Woong , Koo Hong Hoe , Kang Eun-Suk , Ahn Kang Mo , Kim Yae-Jean , Yoo Keon Hee 
Published: May 2021

Abstract
Purpose Hematopoietic stem cell transplantation (HSCT) is the standard method of reconstituting immune function in severe combined immunodeficiency (SCID); however, current conditioning recommendations and optimal cell doses lack consensus. In this study, we investigated HSCT outcomes and immune reconstitution, following minimal dose (MD) HSCT in T cell-negative (T-), B cell-positive (B+), natural killer-cell negative (NK-) SCID patients. Methods We retrospectively reviewed patients with SCID who received HSCT between 2002–2018. Standard dose (SD) and MD were classified based on a total nucleated cell count (TNC) of 1.0 × 10 8 /kg or more and less. Results Seven patients with SCID received HSCT, of which four belonged to the MD group. Patients in the MD group were administered 5 mL or less of bone marrow without conditioning, with median TNC and CD34+ cell counts of 0.49 × 10 8 /kg and 0.62 × 10 6 /kg, respectively. T cells recovered within a year after HSCT, and immunoglobulin supplementation was discontinued at a median of 3.5 months after HSCT in all MD recipients. All MD recipients were alive without disease recurrence at a median of 126.9 months after HSCT, exhibiting donor chimerism in the range of 10.1%–100%. Although grade II–III graft-versus-host diseases occurred, these were manageable in all patients. One of the three patients in the SD group died of cytomegalovirus infection, while another was dependent on intravenous immunoglobulin until 41 months after HSCT. Conclusion In patients with T-B+NK- SCID, sufficient therapeutic effects were safely obtained with minimal dose of bone marrow infusion without conditioning.
Integrated Analysis of tRNA-Derived Small RNAs in Proliferative Human Aortic Smooth Muscle Cells
Authors: Zhao Jian-Zhi , Lin Jia-Jie , Gong Miao , Wang Yu , Liu Ke-Xin , Du Mei-Yang , Li Huan-Huan , Wang Si-Fan , Yang Li-Yun , Sun Bo , Kong Peng , Liu Kun , Li Bin , Sun Shao-Guang 
Published: May 2021

Abstract
Background

Abnormal proliferation of vascular smooth muscle cells (VSMCs) contributes to vascular remodeling diseases. Recently, it has been discovered that tRNA-derived small RNAs (tsRNAs), a new type of non-coding RNAs, are related to the proliferation and migration of VSMCs. tsRNAs regulate target gene expression through miRNA-like function. This study aims to explore the potential of tsRNAs in human aortic smooth muscle cell (HASMC) proliferation.
Methods

High-throughput sequencing was performed to analyze the tsRNA expression profile of proliferative and quiescent HASMCs. Quantitative real-time PCR (qRT-PCR) was performed to validate sequence results and subcellular distribution of AS-tDR-001370, AS-tDR-000067, AS-tDR-009512, and AS-tDR-000076. Based on microRNA-like functions of tsRNAs, we predicted target promoters, mRNAs, and circular RNAs (circRNAs), constructed tsRNA-promoter, tsRNA-mRNA, and circRNA-tsRNA interaction networks. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were performed to reveal the function of target genes. Western blot and EdU incorporation assays were utilized to detect the effect of tsRNAs on HASMC proliferation.
Results

Compared with quiescent HASMCs, 887 up-regulated and 951 down-regulated tsRNAs in proliferative HASMCs were identified (fold change > 2 or < -2, p‑value < 0.05). AS-tDR-001370, AS-tDR-000067, AS-tDR-009512, and AS-tDR-000076 were up-regulated in proliferative HASMCs and were mainly located in the nucleus. Bioinformatics analysis suggested that they involve a variety of terms and pathways related to VSMC proliferation. Knockdown of AS-tDR-000067 promoted the expression of target gene p53 and inhibited HASMC proliferation. Knockdown of AS-tDR-000076 promoted the expression of target gene mitofusin 2 (MFN2) and inhibited HASMC proliferation.
Conclusion

During HASMC proliferation, the expression levels of many tsRNAs are altered. AS-tDR-000067 and AS-tDR-000076 may become a new therapeutic target for vascular remodeling diseases.
Molecular Characterization of a Novel Mycovirus Infecting the Entomopathogenic Fungus Beauveria Bassiana
Authors: Shi Najie , Hu Fei , Wang Ping , Zhang Yuxiang , Zhu Qiuyan , Yang Guogen , Huang Bo 
Published: May 2021

Abstract
The entomopathogenic fungus Beauveria bassiana is used worldwide for its biological control. Seven dsRNA segments were detected from a single Beauveria bassiana strain RCEF 1446. High-throughput sequencing indicated the presence of three mycoviruses in the infected sample. Two known mycoviruses were identified as Beauveria bassiana Victorivirus 1 and Beauveria bassiana polymycovirus 1, and the novel mycovirus was designated as Beauveria bassiana bipartite mycovirus 1 (BbBV1). The complete sequence of the BbBV1 is described here. The mycovirus contains two dsRNA segments. The first dsRNA is 2,026 bp in length and encoding a RNA-dependent RNA polymerase (RdRp) (68.54 kDa), while the second segment encoding a hypothetical protein (35.55 kDa) of unknown function, was 1,810 bp in length. Moreover, the RdRp protein sequences showed the highest identity of 62.89% to Corynespora cassiicola bipartite mycovirus 1. Phylogenetic analysis of the RdRp reveals that the virus represents a distinct lineage of unassigned dsRNA mycoviruses infecting fungi.
Characteristics and Influencing Factors of Tiny Positive Events in the Daily Life of Elderly People Living Alone
Authors: Huang Lili , Jiang Yuqi , Zhen Enjie , Zhu Lijuan , Zhou Chuncong , Lin Wenjun , He Buxin , Chen Xiaolin , Yin Zhiqin 
Published: May 2021

Abstract
Background

The aging population has become a common social problem in China, and the number of elderly people living alone is increasing. The aim of this study was to explore the status and influencing factors of tiny positive events in the daily life among elderly people living alone, and to compare the urban and rural dwelling elderly. The goal of this study was to provide basis developing a more targeted and personalized positive experience strategy and to provide a scientific reference for the prevention of and interventions for loneliness and social isolation.
Methods

A total of 280 old people living alone in Wenzhou were surveyed by a multistage probability sampling method, and a cross-sectional survey was conducted by using a general condition questionnaire and a positive event questionnaire of older persons. The data were analyzed by descriptive statistics, t-tests, analysis of variance, multiple response analysis, chi-square test and multiple linear regression analysis. All statistical analyses were performed using SPSS Statistics 22.0, and the two-tailed level of significance was set at 0.05.
Results

The amount of tiny positive events in the elderly living alone was lower than the medium level, which was concentrated in the dimension of the surrounding environment. The most important positive events showed the characteristics of the hierarchy of needs. Education, economic source, monthly income, two-weeks illness and the frequency of children's visits were the important factors affecting positive events. The urban elderly focused on social and self-worth events, while the rural elderly people focused more on personal and family matters.
Conclusion

In the future, we can combine these characteristics and influencing factors into interventions for the elderly living alone to create more positive events in their daily life and increase their positive experiences.
Nakamurella Leprariae Sp. Nov., Isolated From a Lichen Sample
Authors: An De-Feng , Yang Shao-Juan , Jiang Long-Qian , Wang Xin-Yu , Huang Xiao-Yu , Lang Lei , Chen Xue-Mei , Fan Ming-Qun , Li Gui-Ding , Jiang Ming-Guo , Wang Li-Song , Jiang Cheng-Lin , Jiang Yi 
Published: May 2021

Abstract
A novel actinobacterium, YIM 132084T, was isolated from Lepraria sp. lichen collected from Yunnan province, south-west PR China and identified by a polyphasic taxonomic approach. The strain was Gram-stain-positive, aerobic, catalase-positive, oxidase-negative, non-motile and coccus-shaped. Colonies were round, convex, smooth and light orange yellow in colour. It grew at 10–40 ℃ (optimum 28 ℃), at pH 6.0–11.0 (optimum pH 7.0) and in the presence of 0–4 % NaCl (optimum 0 %). Strain YIM 132084T comprised diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol as the major polar lipids, MK-8(H4) as the predominant menaquinone, and anteiso-C15:0, anteiso-C17:0, iso-C15:0 and iso-C16:0 as major fatty acids. Strain YIM 132084T had meso-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan, and mannose, ribose, glucose and rhamnose as whole-cell sugars. The 16S rRNA gene sequence showed high level of similarity with Nakamurella flavida KCTC 19127T (97. 7%) and Nakamurella flava CGMCC 4.7524T (97.7%). The G + C content of the genomic DNA was 72.4 mol%. Strain YIM 132084T showed an average nucleotide identity value of 76.1 % and 74.9 %, a digital DNA-DNA hybridizations value of 20.9 % and 20.6 % with the reference strains Nakamurella flavida and Nakamurella flava based on draft genome sequences, respectively. The results of the phenotypic, chemotaxonomic and phylogenetic analyses, showed that strain YIM 132084T represents a novel species of the genus Nakamurella, for which the name Nakamurella leprariae sp. nov. is proposed. The type strain is YIM 132084T (= CGMCC 4.7667T = NBRC 114280T = KCTC 49367T).
Annual Changes in Serum Syndecan-1 as a Therapeutic Target and a Biomarker for the Severity Classification of COVID-19
Authors: Ogawa Fumihiro , Oi Yasufumi , Nakajima Kento , Matsumura Reo , Nakagawa Tomoki , Miyagawa Takao , Sakai Kazuya , Saji Ryo , Taniguchi Hayato , Takahashi Kohei , Abe Takeru , Iwashita Masayuki , Nishii Mototsugu , Takeuchi Ichiro 
Published: May 2021

Abstract
Background: Coronavirus disease 2019 (COVID-19) pneumonitis associated with severe respiratory failure is associated with high mortality. The pathogenesis of COVID-19 is associated with microemboli or microvascular endothelial injuries. Here, we report that serum syndecan-1 (SDC-1), a component of the endothelial glycocalyx, may be a biomarker of severity classification for COVID-19 related to endothelial injury.Methods and analysis: We analyzed the data of COVID-19 patients for one year from February 2020 at Yokohama City University Hospital and Yokohama City University Medical Center Hospital. We selected COVID-19 patients who required admission care, including intensive care, and analyzed the classification of severe and critical COVID-19 retrospectively, using various clinical data and laboratory data with serum SDC-1 by ELISA. Results: We analyzed clinical and laboratory data with SDC-1 in five severe COVID-19 and ten critical COVID-19 patients. In the two groups, their backgrounds were almost the same. In laboratory data, the LDH, CHE, and CRP levels showed significant differences in each group (P=0.032, P <0.0001, and P = 0.007, respectively) with no significant differences in coagulation-related factors (platelet, PT-INR, D-dimer, ISTH score; P=0.200, 0.277, 0.655, and 0.36, respectively). For the clinical data, the SOFA score was significantly different from admission day to day 14 of admission (p<0.0001). The SDC-1 levels of critical COVID-19 patients were significantly higher on admission day and all-time course compared with the levels of severe COVID-19 patients (P=0.009 and P <0.0001, respectively).Conclusions: Annual changes in serum SDC-1 levels closely reflect the severity of COVID-19, therefore, serum SDC-1 may be a therapeutic target and a biomarker for the severity classification of Covid-19.
Nonlinear Dynamic Analysis of a Rotor-Porous Air Journal Bearing System With O-Rings Mounted
Authors: Zhang Kang , Feng Kai , Li Wenjun , Song Lijun 
Published: May 2021

Abstract
Porous air journal bearings (PAJBs) with O-rings mounted are regarded as a potential choice to offer air levitation with a large load capacity and optimal start/stop characteristics, which are required in small-size and high-efficiency turbomachinery. However, the coupling of the mechanical properties of the O-rings and lubrication induce strong nonlinear characteristics, thereby affecting the dynamic responses of the rotor. In this study, a numerical model is presented, which is coupled with the excitation frequency-influenced stiffness and damping characteristics of O-rings, the Darcy’s laws, air motion equations established by the air lubrication model, and the rotor and bearing motion equations. The model is well verified by the experimental results. The effect of the different system parameters on the performance of the rotor-PAJB system (RPS) is studied by analyzing the bifurcation diagrams, orbit, Poincaré maps, and FFT plots. Results show that the motion of the rotor is changed between periodic and quasi-periodic with the variation in rotational speed, external supply pressure, bearing clearance, and porous permeability. With O-rings mounted, the stability of RPS can be increased to hinder the rotor motion from changing from periodic to quasi-periodic. The effectiveness in increasing the stability of the RPS for the O-rings with SBR as the material is better than that with NBR but weaker than that with VMQ. Moreover, only a few number of O-rings have better effects on improving the stability of the RPS.
The Neutrophil–Lymphocyte Ratio has Little Impact on Survival in Breast Cancer Patients Treated with Neoadjuvant Chemotherapy Followed by Surgery
Authors: Enomoto Katsuhisa , Fukumoto Satsuki , Goto Hironori , Mori Satoshi , Hara Yukiko , Tada Keiichiro 
Published: May 2021

Abstract
Background: The impact of the neutrophil–lymphocyte ratio (NLR) on the survival outcomes of patients with early-stage breast cancer is controversial. Methods: We reviewed a series of 115 breast cancer patients who received neoadjuvant chemotherapy followed by surgical treatment. The NLR was calculated using a blood sample obtained before neoadjuvant chemotherapy. The relationships of disease-free survival with the NLR as well as other clinicopathological factors were assessed.Results: There was no significant association between the NLR and disease-free survival in these patients. On the other hand, negative estrogen receptor and progesterone receptor statuses, extended nodal involvement, and advanced TNM stage were significant predictors of shorter disease-free survival.Conclusions: The NLR has little impact on the survival of early-stage breast cancer patients treated with neoadjuvant chemotherapy followed by surgical treatment.
Study on the Mechanism of Bushen Jianpi Decoction in the Treatment of T2DM Based on Network Pharmacology
Authors: Luo Dongqiang , Shao Ying , Sun Yong , Du Shuntang , Liu Feng 
Published: May 2021

Abstract
Through the preliminary clinical observation, we had found that Bushen Jianpi decoction (BJD) combined with had better efficacy and less side effects, but its mechanism was not clear. The purpose of this study was to determine its molecular targets and mechanism in T2DM therapy by means of network pharmacology and molecular docking.Results: A total of 144 candidate compounds and 1103 differentially expressed genes were screened, and 43 common targets related to T2DM in BJD were identified. The "TCM-compound-target-disease" network suggested that quercetin, luteolin and kaempferol were the top three compounds. Through protein-protein interaction network, 45 core target genes were identified, including ITGA4, TP53, MYC and so on. GO enrichment showed that genes were significantly enriched in biological processes such as response to oxidative stress, response to lipopolysaccharide, response to molecule of bacterial origin and response to reactive oxygen species. KEGG enrichment showed that there was significant gene enrichment in Fluid shear stress and atherosclerosis, TNF signaling pathway, P13K-Akt signaling pathway, IL-17 signaling pathway and AGE-RAGE signaling pathway in diabetic complications signal pathways. The results of molecular docking showed that the key components of BJD had good binding potential with target genes. Conclusions: BJD may play a role in the treatment of T2DM through anti-inflammation, antioxidation and regulating metabolism, but it still needs to be further confirmed by experiments.Keywords: Network pharmacology, GEO database, Molecular docking, Bushen Jianpi decoction; T2DM
Thymosin Beta 4 as an Early Biomarker in Sepsis Induced Acute Kidney Injury
Authors: Zhang Jiahao , Long Minghui , Sun Zhongyi , Yang Cheng , Jiang Xiaofang , He Li , Su Lianjiu , Peng Zhiyong 
Published: May 2021

Abstract
Background: The incidence of sepsis is high among patients in the intensive care units (ICU) and acute kidney injury (AKI) is a common complication of sepsis that contributes to increased mortality. Thymosin beta-4 (Tβ4) is an actin-sequestering protein that can prevent inflammation and fibrosis in several tissues. However, its functions in septic AKI remain unknown.Methods: 98 consecutive hospitalized patients with confirmed sepsis were enrolled. Demographics, comorbidities, laboratory findings, and outcomes were collected and analyzed. Serum Tβ4 levels at ICU admission were measured and analyzed for evaluating the probability of AKI using the logistic regression. In addition, the effects of exogenous Tβ4 on kidney injury was also conducted in mice where a sepsis model was induced by lipopolysaccharide (LPS) intraperitoneal injection. Results: Of the 98 patients with sepsis, 47 (48%) developed AKI. Patients with hypertension, diabetes, higher body mass index (BMI) and Sequential Organ Failure Assessment (SOFA) score were more likely to develop AKI. Among patients with AKI, hemoglobin, and Tβ4 were significantly decreased. Multivariate analysis showed decreased Tβ4, high SOFA, and high BMI to be independent risk factors for AKI in patients with sepsis. The overall mortality rate of the 98 septic patients was 20.4%, and the mortality rate of those with AKI was 29.8%. Kaplan-Meier analysis demonstrated that patients with AKI had a significantly higher risk of death. In particular, increasing AKI severity was associated with an increased risk of death. Furthermore, exogenous Tβ4 could reduce renal apoptosis and attenuated renal dysfunction, as well as reducing systemic inflammatory response through the prevention of the activation of NF-κB pathway in the sepsis model.Conclusions: The combination of Tβ4, SOFA, and BMI could allow for timely detection of septic AKI. Exogenous Tβ4 could prevent kidney injury in sepsis.
Proteomic Analysis of Eluted Antigens from Crude Somatic Antigens of Trichinella Spiralis Muscle Larva by Igg-ELISA, Two-Dimensional Gel Electrophoresis, and Mass Spectrometry
Authors: Supcharoengoon Utsanee , Watthanakulpanich Dorn , Limpanont Yanin , Adisakwattana Poom , Reamtong Onrapak , Zhiyue Lv , Dekumyoy Paron 
Published: May 2021

Abstract
Background: Trichinellosis is caused by Trichinella spiralis muscle larvae (ML), which in swine is the main source of transmission. Trichinellosis is detected by enzyme-linked immunosorbent assay (ELISA) using excretory–secretory antigens (ESAg). Preparation of ESAg is difficult, and it produces a low content. Furthermore, the sensitivity and specificity of the test depends on the quality of the antigen that is produced in each batch. Crude somatic antigens (CSAg) of T. spiralis ML at molecular weights (MWs) of 101, 79, and 43 kDa were previously positive for swine trichinellosis sera, with no cross-reaction observed for normal sera, except that 79 and 43 kDa each reacted with one of the coccidiosis and with mixed infections containing trichuriasis and coccidiosis. Therefore, the current study aimed to obtain eluted antigens of T. spiralis ML CSAg, which were analyzed by IgG-ELISA for detecting swine trichinellosis and identification by two-dimensional polyacrylamide gel electrophoresis (2-DE) and liquid chromatography-tandem mass spectrometry (LC MS-MS).Methods: In this study, T. spiralis larvae CSAg at 101, 79, and 43 kDa (TsCSAg-101, TsCSAg-79, and TsCSAg-43, respectively) were eluted from sodium dodecyl sulfate–polyacrylamide gel electrophoresis. The eluted antigens were analyzed by IgG-ELISA for sensitivities and specificities. In addition, three specific antigens were identified by 2-DE and LC MS-MS.Results: Sensitivity of IgG-ELISA using three eluted antigens was persistent at 100%. Specificities were 90.63%, 95.54%, and 97.77% following TsCSAg-101, TsCSAg-79, and TsCSAg-43, respectively. The LC MS-MS results showed that 18/20 spots of the antigens were identified for 11 different proteins. One protein has several isoforms; for example, a serine proteinase and the phosphoenolpyruvate carboxykinase protein.Conclusions: TsCSAg-43 showed the highest specificity compared to the other two eluted antigens, which indicated that these specific proteins (a 45k antigen–trichina [fragment], a DNA topoisomerase 2-alpha, an antigen targeted by protective antibodies, and a conserved hypothetical protein [gi339234223]) should be developed and produced in large volumes in further study.
Efficacy of Yoga Nidra vs Supine Relaxation Technique on Depression, Anxiety, Insomnia in frontline COVID-19 Health Care Workers: A Randomized Controlled Trial
Authors: Gunjiganvi Mallikarjun , Gurjar Mohan , Mishra Prabhakar 
Published: May 2021

Abstract
Background

Mental health disturbances have been well documented due to COVID-19 pandemic. There was surge in literature about use of various Mind Body Therapies during pandemic to curb these disturbances. But none have been studied to prospectively during COVID-19 pandemic. Hence, present study was planned to evaluate the efficacy of Yoganidra in frontline Health care workers (HCW) during pandemic using online and digital tools.
Methods

This open label randomized trial consisting of 2 arms (Yoganidra and Supine Relaxation technique [SRT]) was conducted at Level- III COVID care centre involving dedicated frontline HCWs. Online YouTube digital platform was used deliver the respective intervention to study participants. Daily reminders were sent using digital social media. Each intervention lasted for 30min a day. Participants completing > 9 days of practice were included for analysis. Primary outcome was changes in scores of Physical Health Quality (PHQ)-9, Generalized Anxiety Score (GAD)-7, Insomnia Severity Index (ISI) scale. Secondary outcomes were rate of recruitment, days of intervention practice, satisfaction of digital contents. Statistical analysis was carried out using SPSS v.23.
Results

Of 139 HCWs contacted, 79 HCWs (40 in SRT and 39 in Yoganidra) consented and were recruited into study. After withdrawal and incomplete practice days, 32 in SRT group and 30 in Yoganidra group were included for final analysis. Demographics and pre-intervention scores of PHQ-9, GAD-7, ISI were comparable at baseline. After completion of study, there were significant reduction in scores of PHQ-9, GAD-7 and ISI in Yoganidra 0.002 vs 0.064, < 0.001 vs 0.123, < 0.001 vs 0.828 respectively and effect size was 0.041, 0.013 and 0.024 respectively compared to SRT group. Recruitment rate was 56.8%, 78% of participants completed > 9 days of practice and 100% satisfaction among participants.
Conclusion

Yoganidra significantly reduces depression, anxiety and insomnia scores among frontline COVID HCWs compared to SRT using virtual session platform. It is also feasible to conduct the virtual sessions of Yoga Nidra using digital platform.
Trial Registration:

Clinical Trials Registry of India REF/CTRI/2020/07/026609, (ctri.nic.in/clinicaltrials/login.php, number REF/CTRI/2020/07/026609)