bluuebunny/crossref_metadata_2025_split huggingface dataset:
CHARGE syndrome protein CHD7 regulates epigenomic activation of enhancers in granule cell precursors and gyrification of the cerebellum
Authors: Reddy Naveen C. , Majidi Shahriyar P. , Kong Lingchun , Nemera Mati , Ferguson Cole J. , Moore Michael , Goncalves Tassia M. , Liu Hai-Kun , Fitzpatrick James A. J. , Zhao Guoyan , Yamada Tomoko , Bonni Azad , Gabel Harrison W. 
Published: September 2021

AbstractRegulation of chromatin plays fundamental roles in the development of the brain. Haploinsufficiency of the chromatin remodeling enzyme CHD7 causes CHARGE syndrome, a genetic disorder that affects the development of the cerebellum. However, how CHD7 controls chromatin states in the cerebellum remains incompletely understood. Using conditional knockout of CHD7 in granule cell precursors in the mouse cerebellum, we find that CHD7 robustly promotes chromatin accessibility, active histone modifications, and RNA polymerase recruitment at enhancers. In vivo profiling of genome architecture reveals that CHD7 concordantly regulates epigenomic modifications associated with enhancer activation and gene expression of topologically-interacting genes. Genome and gene ontology studies show that CHD7-regulated enhancers are associated with genes that control brain tissue morphogenesis. Accordingly, conditional knockout of CHD7 triggers a striking phenotype of cerebellar polymicrogyria, which we have also found in a case of CHARGE syndrome. Finally, we uncover a CHD7-dependent switch in the preferred orientation of granule cell precursor division in the developing cerebellum, providing a potential cellular basis for the cerebellar polymicrogyria phenotype upon loss of CHD7. Collectively, our findings define epigenomic regulation by CHD7 in granule cell precursors and identify abnormal cerebellar patterning upon CHD7 depletion, with potential implications for our understanding of CHARGE syndrome.
Multiplex suppression of four quadruplet codons via tRNA directed evolution
Authors: DeBenedictis Erika A. , Carver Gavriela D. , Chung Christina Z. , Söll Dieter , Badran Ahmed H. 
Published: September 2021

AbstractGenetic code expansion technologies supplement the natural codon repertoire with assignable variants in vivo, but are often limited by heterologous translational components and low suppression efficiencies. Here, we explore engineered Escherichia coli tRNAs supporting quadruplet codon translation by first developing a library-cross-library selection to nominate quadruplet codon–anticodon pairs. We extend our findings using a phage-assisted continuous evolution strategy for quadruplet-decoding tRNA evolution (qtRNA-PACE) that improved quadruplet codon translation efficiencies up to 80-fold. Evolved qtRNAs appear to maintain codon-anticodon base pairing, are typically aminoacylated by their cognate tRNA synthetases, and enable processive translation of adjacent quadruplet codons. Using these components, we showcase the multiplexed decoding of up to four unique quadruplet codons by their corresponding qtRNAs in a single reporter. Cumulatively, our findings highlight how E. coli tRNAs can be engineered, evolved, and combined to decode quadruplet codons, portending future developments towards an exclusively quadruplet codon translation system.
Collective patterns of social diffusion are shaped by individual inertia and trend-seeking
Authors: Ye Mengbin , Zino Lorenzo , Mlakar Žan , Bolderdijk Jan Willem , Risselada Hans , Fennis Bob M. , Cao Ming 
Published: September 2021

AbstractSocial conventions change when individuals collectively adopt an alternative over the status quo, in a process known as social diffusion. Our repeated trials of a multi-round experiment provided data that helped motivate the proposal of an agent-based model of social diffusion that incorporates inertia and trend-seeking, two behavioural mechanisms that are well documented in the social psychology literature. The former causes people to stick with their current decision, the latter creates sensitivity to population-level changes. We show that such inclusion resolves the contradictions of existing models, allowing to reproduce patterns of social diffusion which are consistent with our data and existing empirical observations at both the individual and population level. The model reveals how the emergent population-level diffusion pattern is critically shaped by the two individual-level mechanisms; trend-seeking guarantees the diffusion is explosive after the diffusion process takes off, but inertia can greatly delay the time to take-off.
Chemically driven energetic molecular ferroelectrics
Authors: Hu Yong , Liu Zhiyu , Wu Chi-Chin , Gottfried Jennifer L. , Pesce-Rodriguez Rose , Walck Scott D. , Chung Peter W. , Ren Shenqiang 
Published: September 2021

AbstractChemically driven thermal wave triggers high energy release rate in covalently-bonded molecular energetic materials. Molecular ferroelectrics bridge thermal wave and electrical energy by pyroelectric associated with heating frequency, thermal mass and heat transfer. Herein we design energetic molecular ferroelectrics consisting of imidazolium cations (energetic ion) and perchlorate anions (oxidizer), and describe its thermal wave energy conversion with a specific power of 1.8 kW kg−1. Such a molecular ferroelectric crystal shows an estimated detonation velocity of 7.20 ± 0.27 km s−1 comparable to trinitrotoluene and hexanitrostilbene. A polarization-dependent heat transfer and specific power suggests the role of electron-phonon interaction in tuning energy density of energetic molecular ferroelectrics. These findings represent a class of molecular ferroelectric energetic compounds for emerging energy applications demanding high power density.
Low-field onset of Wannier-Stark localization in a polycrystalline hybrid organic inorganic perovskite
Authors: Berghoff Daniel , Bühler Johannes , Bonn Mischa , Leitenstorfer Alfred , Meier Torsten , Kim Heejae 
Published: September 2021

AbstractMethylammonium lead iodide perovskite (MAPbI3) is renowned for an impressive power conversion efficiency rise and cost-effective fabrication for photovoltaics. In this work, we demonstrate that polycrystalline MAPbI3s undergo drastic changes in optical properties at moderate field strengths with an ultrafast response time, via transient Wannier Stark localization. The distinct band structure of this material - the large lattice periodicity, the narrow electronic energy bandwidths, and the coincidence of these two along the same high-symmetry direction – enables relatively weak fields to bring this material into the Wannier Stark regime. Its polycrystalline nature is not detrimental to the optical switching performance of the material, since the least dispersive direction of the band structure dominates the contribution to the optical response, which favors low-cost fabrication. Together with the outstanding photophysical properties of MAPbI3, this finding highlights the great potential of this material in ultrafast light modulation and novel photonic applications.
Extremely anisotropic van der Waals thermal conductors
Authors: Kim Shi En , Mujid Fauzia , Rai Akash , Eriksson Fredrik , Suh Joonki , Poddar Preeti , Ray Ariana , Park Chibeom , Fransson Erik , Zhong Yu , Muller David A. , Erhart Paul , Cahill David G. , Park Jiwoong 
Published: September 2021

AbstractThe densification of integrated circuits requires thermal management strategies and high thermal conductivity materials1–3. Recent innovations include the development of materials with thermal conduction anisotropy, which can remove hotspots along the fast-axis direction and provide thermal insulation along the slow axis4,5. However, most artificially engineered thermal conductors have anisotropy ratios much smaller than those seen in naturally anisotropic materials. Here we report extremely anisotropic thermal conductors based on large-area van der Waals thin films with random interlayer rotations, which produce a room-temperature thermal anisotropy ratio close to 900 in MoS2, one of the highest ever reported. This is enabled by the interlayer rotations that impede the through-plane thermal transport, while the long-range intralayer crystallinity maintains high in-plane thermal conductivity. We measure ultralow thermal conductivities in the through-plane direction for MoS2(57 ± 3 mW m−1 K−1) and WS2(41 ± 3 mW m−1 K−1) films, and we quantitatively explain these values using molecular dynamics simulations that reveal one-dimensional glass-like thermal transport. Conversely, the in-plane thermal conductivity in these MoS2films is close to the single-crystal value. Covering nanofabricated gold electrodes with our anisotropic films prevents overheating of the electrodes and blocks heat from reaching the device surface. Our work establishes interlayer rotation in crystalline layered materials as a new degree of freedom for engineering-directed heat transport in solid-state systems.
Diagnostic performance of the Abbott RealTime MTB assay for tuberculosis diagnosis in people living with HIV
Authors: Saavedra Belén , Mambuque Edson , Gomes Neide , Nguenha Dinis , Mabunda Rita , Faife Luis , Langa Ruben , Munguambe Shilzia , Manjate Filomena , Cossa Anelsio , Scott Lesley , García-Basteiro Alberto L. 
Published: September 2021

AbstractStrengthening tuberculosis diagnosis is an international priority and the advocacy for multi-disease testing devices raises the possibility of improving laboratory efficiency. However, the advantages of centralized platforms might not translate into real improvements under operational conditions. This study aimed to evaluate the field use of the Abbott RealTime MTB (RT-MTB) and Xpert MTB/RIF assays, in a large cohort of HIV-positive and TB presumptive cases in Southern Mozambique. Over a 6-month period, 255 HIV-positive TB presumptive cases were consecutively recruited in the high TB/HIV burden district of Manhiça. The diagnostic performance of both assays was evaluated against two different reference standards: a microbiological gold standard (MGS) and a composite reference standard (CRS). Results from the primary analysis (MGS) showed improved sensitivity (Se) and reduced specificity (Sp) for the Abbott RT-MTB assay compared to the Xpert MTB/RIF (RT-MTB Se: 0.92 (95% CI: 0.75;0.99) vs Xpert Se: 0.73 (95% CI: 0.52;0.88) p value = 0.06; RT-MTB Sp: 0.80 (0.72;0.86) vs Xpert Sp: 0.96 (0.92;0.99) p value < 0.001). The lower specificity may be due to cross-reactivity with non-tuberculous mycobacteria (NTMs), the detection of non-viable MTBC, or the identification of true TB cases missed by the gold standard.
Experience of symptom control, anxiety and associating factors in a palliative care unit evaluated with Support Team Assessment Schedule Japanese version
Authors: Ito Tetsuya , Tomizawa Emi , Yano Yuki , Takei Kiyozumi , Takahashi Naoko , Shaku Fumio 
Published: September 2021

AbstractVarious physical and psychosocial difficulties including anxiety affect cancer patients. Patient surroundings also have psychological effects on caregiving. Assessing the current status of palliative care intervention, specifically examining anxiety and its associated factors, is important to improve palliative care unit (PCU) patient quality of life (QOL). This study retrospectively assessed 199 patients admitted to a PCU during August 2018–June 2019. Data for symptom control, anxiety level, disease insight, and communication level obtained using Support Team Assessment Schedule Japanese version (STAS-J) were evaluated on admission and after 2 weeks. Palliative Prognostic Index (PPI) and laboratory data were collected at admission. Patient anxiety was significantly severer and more frequent in groups with severer functional impairment (p = 0.003) and those requiring symptom control (p = 0.006). Nevertheless, no relation was found between dyspnea and anxiety (p = 0.135). Patients with edema more frequently experienced anxiety (p = 0.068). Patient survival was significantly shorter when family anxiety was higher after 2 weeks (p = 0.021). Symptoms, edema, and disabilities in daily living correlate with patient anxiety. Dyspnea is associated with anxiety, but its emergence might be attributable mainly to physical factors in this population. Family members might sensitize changes reflecting worsened general conditions earlier than the patients.
Asymmetric Little–Parks oscillations in full shell double nanowires
Authors: Vekris Alexandros , Estrada Saldaña Juan Carlos , de Bruijckere Joeri , Lorić Sara , Kanne Thomas , Marnauza Mikelis , Olsteins Dags , Nygård Jesper , Grove-Rasmussen Kasper 
Published: September 2021

AbstractLittle–Parks oscillations of a hollow superconducting cylinder are of interest for flux-driven topological superconductivity in single Rashba nanowires. The oscillations are typically symmetric in the orientation of the applied magnetic flux. Using double InAs nanowires coated by an epitaxial superconducting Al shell which, despite the non-centro-symmetric geometry, behaves effectively as one hollow cylinder, we demonstrate that a small misalignment of the applied parallel field with respect to the axis of the nanowires can produce field-asymmetric Little–Parks oscillations. These are revealed by the simultaneous application of a magnetic field perpendicular to the misaligned parallel field direction. The asymmetry occurs in both the destructive regime, in which superconductivity is destroyed for half-integer quanta of flux through the shell, and in the non-destructive regime, where superconductivity is depressed but not fully destroyed at these flux values.
Fluorescence quenching based detection of nitroaromatics using luminescent triphenylamine carboxylic acids
Authors: Mishra Aamnayee , Dheepika R. , Parvathy P. A. , Imran P. M. , Bhuvanesh N. S. P. , Nagarajan S. 
Published: September 2021

AbstractDetection of nitroaromatics employing greener techniques has been one of the most active research fields in chemistry. A series of triphenylamine (TPA) functionalized carboxylic acids were synthesized and characterized using various spectroscopic techniques including single-crystal X-ray diffraction analysis. The interaction of carboxylic acid-decorated TPAs with nitroaromatic compounds was photophysically explored using absorption and emission spectroscopy. Stern–Volmer plot accounts for the appreciable quenching constant of the TPA-acids. Density functional theory calculations were carried out to study the new compounds' frontier molecular orbital energy levels and the possible interactions with picrate anion and revealed an unusual charge transfer interaction between acids and picrate anion. The contact mode detection shows the TPA-acids can be used as dip-strip sensors for picric acid detection.
Early type 1 diabetes aggravates renal ischemia/reperfusion-induced acute kidney injury
Authors: de Ponte Mariana Charleaux , Cardoso Vanessa Gerolde , Gonçalves Guilherme Lopes , Costa-Pessoa Juliana Martins , Oliveira-Souza Maria 
Published: September 2021

AbstractThe present study aimed to investigate the interaction between early diabetes and renal IR-induced AKI and to clarify the mechanisms involved. C57BL/6J mice were assigned to the following groups: (1) sham-operated; (2) renal IR; (3) streptozotocin (STZ—55 mg/kg/day) and sham operation; and (4) STZ and renal IR. On the 12th day after treatments, the animals were subjected to bilateral IR for 30 min followed by reperfusion for 48 h, at which time the animals were euthanized. Renal function was assessed by plasma creatinine and urea levels, as well urinary protein contents. Kidney morphology and gene and protein expression were also evaluated. Compared to the sham group, renal IR increased plasma creatinine, urea and albuminuria levels and decreased Nphs1 mRNA expression and nephrin and WT1 protein staining. Tubular injury was observed with increased Havcr1 and Mki67 mRNA expression accompanied by reduced megalin staining. Renal IR also resulted in increased SQSTM1 protein expression and increased proinflammatory and profibrotic factors mRNA expression. Although STZ treatment resulted in hyperglycemia, it did not induce significant changes in renal function. On the other hand, STZ treatment aggravated renal IR-induced AKI by exacerbating renal dysfunction, glomerular and tubular injury, inflammation, and profibrotic responses. Thus, early diabetes constitutes a relevant risk factor for renal IR-induced AKI.
Inferring entire spiking activity from local field potentials
Authors: Ahmadi Nur , Constandinou Timothy G. , Bouganis Christos-Savvas 
Published: September 2021

AbstractExtracellular recordings are typically analysed by separating them into two distinct signals: local field potentials (LFPs) and spikes. Previous studies have shown that spikes, in the form of single-unit activity (SUA) or multiunit activity (MUA), can be inferred solely from LFPs with moderately good accuracy. SUA and MUA are typically extracted via threshold-based technique which may not be reliable when the recordings exhibit a low signal-to-noise ratio (SNR). Another type of spiking activity, referred to as entire spiking activity (ESA), can be extracted by a threshold-less, fast, and automated technique and has led to better performance in several tasks. However, its relationship with the LFPs has not been investigated. In this study, we aim to address this issue by inferring ESA from LFPs intracortically recorded from the motor cortex area of three monkeys performing different tasks. Results from long-term recording sessions and across subjects revealed that ESA can be inferred from LFPs with good accuracy. On average, the inference performance of ESA was consistently and significantly higher than those of SUA and MUA. In addition, local motor potential (LMP) was found to be the most predictive feature. The overall results indicate that LFPs contain substantial information about spiking activity, particularly ESA. This could be useful for understanding LFP-spike relationship and for the development of LFP-based BMIs.
Optimization of epilepsy surgery through virtual resections on individual structural brain networks
Authors: Nissen Ida A. , Millán Ana P. , Stam Cornelis J. , van Straaten Elisabeth C. W. , Douw Linda , Pouwels Petra J. W. , Idema Sander , Baayen Johannes C. , Velis Demetrios , Van Mieghem Piet , Hillebrand Arjan 
Published: September 2021

AbstractThe success of epilepsy surgery in patients with refractory epilepsy depends upon correct identification of the epileptogenic zone (EZ) and an optimal choice of the resection area. In this study we developed individualized computational models based upon structural brain networks to explore the impact of different virtual resections on the propagation of seizures. The propagation of seizures was modelled as an epidemic process [susceptible-infected-recovered (SIR) model] on individual structural networks derived from presurgical diffusion tensor imaging in 19 patients. The candidate connections for the virtual resection were all connections from the clinically hypothesized EZ, from which the seizures were modelled to start, to other brain areas. As a computationally feasible surrogate for the SIR model, we also removed the connections that maximally reduced the eigenvector centrality (EC) (large values indicate network hubs) of the hypothesized EZ, with a large reduction meaning a large effect. The optimal combination of connections to be removed for a maximal effect were found using simulated annealing. For comparison, the same number of connections were removed randomly, or based on measures that quantify the importance of a node or connection within the network. We found that 90% of the effect (defined as reduction of EC of the hypothesized EZ) could already be obtained by removing substantially less than 90% of the connections. Thus, a smaller, optimized, virtual resection achieved almost the same effect as the actual surgery yet at a considerably smaller cost, sparing on average 27.49% (standard deviation: 4.65%) of the connections. Furthermore, the maximally effective connections linked the hypothesized EZ to hubs. Finally, the optimized resection was equally or more effective than removal based on structural network characteristics both regarding reducing the EC of the hypothesized EZ and seizure spreading. The approach of using reduced EC as a surrogate for simulating seizure propagation can suggest more restrictive resection strategies, whilst obtaining an almost optimal effect on reducing seizure propagation, by taking into account the unique topology of individual structural brain networks of patients.
Mapping of static magnetic fields near the surface of mobile phones
Authors: Zastko L. , Makinistian L. , Tvarožná A. , Ferreyra F. L. , Belyaev I. 
Published: September 2021

AbstractWhether the use of mobile phones (MP) represents a health hazard is still under debate. As part of the attempts to resolve this uncertainty, there has been an extensive characterization of the electromagnetic fields MP emit and receive. While the radiofrequencies (RF) have been studied exhaustively, the static magnetic fields (SMF) have received much less attention, regardless of the fact there is a wealth of evidence demonstrating their biological effects. We performed 2D maps of the SMF at several distances from the screen of 5 MP (models between 2013 and 2018) using a tri-axis magnetometer. We built a mathematical model to fit our measurements, extrapolated them down to the phones’ screen, and calculated the SMF on the skin of a 3D head model, showing that exposure is in the µT to mT range. Our literature survey prompts the need of further research not only on the biological effects of SMF and their gradients, but also on their combination with extremely low frequency (ELF) and RF fields. The study of combined fields (SMF, ELF, and RF) as similar as possible to the ones that occur in reality should provide a more sensible assessment of potential risks.
Temporal trends in tolvaptan use after revision of national heart failure guidelines in Japan
Authors: Yamazaki Yusuke , Shiraishi Yasuyuki , Kohsaka Shun , Nagatomo Yuji , Fukuda Keiichi , Kohno Takashi , Yoshikawa Tsutomu 
Published: September 2021

AbstractWithin no definite diuretic protocol for acute heart failure (AHF) patients and its variation in regional clinical guidelines, the latest national guidelines in Japan commends use of tolvaptan in diuretic-resistant patients. This study aimed to examine trends in tolvaptan usage and associated outcomes of AHF patients requiring hospitalization. Between April, 2018 and October, 2019, 1343 consecutive AHF patients (median 78 [69–85] year-old) were enrolled in a prospective, multicenter registry in Japan. Trends over time in tolvaptan usage, along with the severity of heart failure status based on the Get With The Guideline-Heart Failure [GWTG-HF] risk score, and in-hospital outcomes were investigated. During the study period, tolvaptan usage has increased from 13.0 to 28.7% over time (p for trend = 0.07), and 49.4% started tolvaptan within 3 days after admission. The GWTG-HF risk score in the tolvaptan group has significantly decreased over time, while that in the non-tolvaptan group has unchanged. There were no differences in the in-hospital mortality rate between the patients with and without tolvaptan (6.7% vs. 5.8%). After revision of the Japanese clinical practice guidelines for AHF in March 2018, tolvaptan usage for AHF patients has steadily increased. However, in-hospital outcomes including mortality do not seem to be affected.