bluuebunny/crossref_metadata_2025_split huggingface dataset:
Diabetes self-care practices and resilience in the Brazilian COVID-19 pandemic: results of a web survey: DIABETESvid
Authors: Binhardi Bárbara Aparecida , de Souza Teixeira Carla Regina , de Almeida-Pititto Bianca , Barbosa-Junior Francisco , Franco Laercio Joel , de Melo Karla Fabiana Santana , Malerbi Domingos Augusto Cherino , de Oliveira Rinaldo Eduardo Machado 
Published: August 2021

Abstract
Background
The world guidance on the measures of social distancing for prevention of COVID-19 has changed the daily habits of great part of the population, and this could influence the care and resilience with diabetes during situations of adversity. This study aimed at assessing the characteristics of diabetic individuals and self-care practices and resilience with diabetes in the context of the COVID-19 pandemic in Brazil.

Methods
This is a cross-sectional web survey study carried out among adults with diabetes, in which a structured 43-item questionnaire was conducted on the REDCap plataform, including the Diabetes Self-Care Activities Questionnaire and Connor-Davidson Resilience Scale, to measure socio-demographic and clinical characteristics. The web survey was disseminated through the main social media and data were collected from September 1st to October 19th, 2020. Data analysis was performed according to type of diabetes mellitus (DM) and at a significance level of 5% (p < 0.05).

Results
Of the 1633 participants, 67.5% were women, 43.2% aged between 35 and 59 years old, 68.0% lived in the south-eastern region of Brazil, 57.1% had a high education level, 49% reported to have DM1 and 140 participants reported to have had COVID-19. Diabetes care mostly involved the use of medications (93%), whereas the least used ones were physical activity (24.6%) and examination of the shoes (35.7%). About 40% of the participants reported to be followed up by telemedicine, 61.5% monitored the glycaemic levels, 61.2% followed a healthy diet and 43.4% left home only to go to the supermarket and drugstore. The mean resilience was 25.4 (SD = 7.7).

Conclusions
In Brazil, individuals with diabetes followed social distancing and maintained their medication treatment for DM. However, practice of physical activity and foot examination was little followed by the participants, who also had a low level of resilience. These findings showed the importance of patient follow-up in the healthcare services, meaning that telemedicine should be improved and support provided for adaptation in view of the therapeutic setbacks.
Multiple schwannomas of the facial nerve mimicking cervical lymphoma: a case report
Authors: Kühn Jan Philipp , Wagner Mathias , Bozzato Alessandro , Linxweiler Maximilian 
Published: August 2021

AbstractBackgroundIn this report, we describe the first case in literature of a patient with multiple schwannomas of the marginal mandibular branch of the facial nerve.Case presentationA Caucasian patient presented with a sudden onset of left lower facial nerve palsy House–Brackmann score III for 1 month. Computed tomography imaging was performed to exclude a cerebral event and revealed multiple tumors within the left parotid gland. Duplex ultrasound and magnetic resonance imaging scans delineated multiple, hypoechoic tumors, round in shape and well defined without a hilar structure along the left mandible. For histological verification, a left-side partial parotidectomy and extirpation of an intraparotideal node was performed with use of a nerve-integrity monitor. Histomorphological analysis of the resected tissue revealed a benign schwannoma. Facial nerve function remained unchanged since the operation. The size of the nonresected tumors is currently monitored regularly by ultrasonography. Fibromatosis has been excluded.ConclusionsIf multiple tumors occur in the parotid gland and the angle of the jaw, schwannomas need to be considered as a differential diagnosis. To plan the right diagnostic surgical intervention and prevent nerve damage, a thorough ultrasound examination is essential in preoperative diagnostic work-up for any suspicious lesion of the parotid gland and jaw region.
Acute pharmacogenetic dystonic reactions in a family with the CYP2D6 *41 allele: a case report
Authors: Wong Darice Y. , Fogel Brent L. 
Published: August 2021

Abstract
Background
Dystonia is a known neurological complication of certain medications; however, the mechanism behind such effects is often undetermined. Similarly, the clinical pharmacogenomic effects associated with various alleles of the cytochrome P450 family of proteins, and their role in acute dystonic reactions, are also presently unknown.

Case presentation
We describe a woman presenting with acute dystonic reactions to ondansetron, prochlorperazine, and metoclopramide followed by persistent focal dystonia. A similar family history was reported in her siblings and her father to prochlorperazine, drugs all metabolized by the cytochrome P450 2D6 (CYP2D6) enzyme. Pharmacogenomic testing indicated the patient was heterozygous for the intermediate metabolizer *41 allele (CYP2D6 2988G>A, NM_000106.6:c.985+39G>A, rs28371725). Her father was homozygous for this CYP2D6 *41 allele, and consequently, her siblings were obligate carriers.

Conclusions
The metabolism of ondansetron, metoclopramide, or prochlorperazine in patients with the *41 CYP2D6 allele has not been studied. In this family, clinical evidence implicates the *41 CYP2D6 allele as causing extrapyramidal adverse pharmacologic reactions. Patients with a family history of medication-induced dystonia involving these medications should be considered for pharmacogenomic testing, and patients carrying the *41 CYP2D6 allele should consider reduction or avoidance of CYP2D6-mediated medications to minimize the potential risk of adverse extrapyramidal effects.
cGMP-grade human iPSC-derived retinal photoreceptor precursor cells rescue cone photoreceptor damage in non-human primates
Authors: Lingam Swathi , Liu Zengping , Yang Binxia , Wong Wendy , Parikh Bhav Harshad , Ong Jun Yi , Goh Debbie , Wong Daniel Soo Lin , Tan Queenie Shu Woon , Tan Gavin S. W. , Holder Graham E. , Regha Kakkad , Barathi Veluchamy Amutha , Hunziker Walter , Lingam Gopal , Zeng Xianmin , Su Xinyi 
Published: August 2021

Abstract
Background
Retinal regenerative therapies hold great promise for the treatment of inherited retinal degenerations (IRDs). Studies in preclinical lower mammal models of IRDs have suggested visual improvement following retinal photoreceptor precursors transplantation, but there is limited evidence on the ability of these transplants to rescue retinal damage in higher mammals. The purpose of this study was to evaluate the therapeutic potential of photoreceptor precursors derived from clinically compliant induced pluripotent stem cells (iPSCs).

Methods
Photoreceptor precursors were sub-retinally transplanted into non-human primates (Macaca fascicularis). The cells were transplanted both in naïve and cobalt chloride-induced retinal degeneration models who had been receiving systemic immunosuppression for one week prior to the procedure. Optical coherence tomography, fundus autofluorescence imaging, electroretinography, ex vivo histology and immunofluorescence staining were used to evaluate retinal structure, function and survival of transplanted cells.

Results
There were no adverse effects of iPSC-derived photoreceptor precursors on retinal structure or function in naïve NHP models, indicating good biocompatibility. In addition, photoreceptor precursors injected into cobalt chloride-induced retinal degeneration NHP models demonstrated an ability both to survive and to mature into cone photoreceptors at 3 months post-transplant. Optical coherence tomography showed restoration of retinal ellipsoid zone post-transplantation.

Conclusions
These findings demonstrate the safety and therapeutic potential of clinically compliant iPSC-derived photoreceptor precursors as a cell replacement source for future clinical trials.
Effective asymmetric preparation of (R)-1-[3-(trifluoromethyl)phenyl]ethanol with recombinant E. coli whole cells in an aqueous Tween-20/natural deep eutectic solvent solution
Authors: Zhuang Wenjin , Liu Hanyu , Zhang Ying , He Junyao , Wang Pu 
Published: August 2021

Abstract(R)-1-[3-(Trifluoromethyl)phenyl]ethanol ((R)-MTF-PEL) is an important chiral building block for the synthesis of a neuroprotective compound, (R)-3-(1-(3-(trifluoromethyl)phenyl)ethoxy)azetidine-1-carboxamide. In this work, an effective whole-cell-catalyzed biotransformation was developed to produce (R)-MTF-PEL, and its productivity was increased by medium engineering strategy. The recombinant E. coli BL21(DE3)-pET28a(+)-LXCAR-S154Y variant affording carbonyl reductase was adopted for the reduction of 3'-(trifluoromethyl)acetophenone to (R)-MTF-PEL with enantiomeric excess (ee) > 99.9%. The addition of 0.6% Tween-20 (w/v) boosted the bioreduction, because the substrate concentration was increased by 4.0-fold than that in the neat buffer solution. The biocatalytic efficiency was further enhanced by introducing choline chloride: lysine (ChCl:Lys, molar ratio of 1:1) in the reaction medium, because the product yield reached 91.5% under 200 mM substrate concentration in the established Tween-20/ChCl:Lys-containing system, which is the highest ever reported for (R)-MTF-PEL production. The optimal reduction conditions were as follows: 4% (w/v) ChCl:Lys, 12.6 g (DCW)/L recombinant E. coli cells, pH 7.0, 30 ℃ and 200 rpm, reaction for 18 h. The combined strategy of surfactant and NADES has great potential in the biocatalytic process and the synthesis of chiral alcohols.
A nonlinear fractional Rayleigh–Stokes equation under nonlocal integral conditions
Authors: Luc Nguyen Hoang , Long Le Dinh , Van Ho Thi Kim , Nguyen Van Thinh 
Published: August 2021

AbstractIn this paper, we study the fractional nonlinear Rayleigh–Stokes equation under nonlocal integral conditions, and the existence and uniqueness of the mild solution to our problem are considered. The ill-posedness of the mild solution to the problem recovering the initial value is also investigated. To tackle the ill-posedness, a regularized solution is constructed by the Fourier truncation method, and the convergence rate to the exact solution of this method is demonstrated.
Dietary phytochemicals as a promising nutritional strategy for sarcopenia: a systematic review and meta-analysis of randomized controlled trials
Authors: Jeong Hye Yun , Kwon Oran 
Published: August 2021

AbstractThe decline in skeletal muscle mass and strength, also called sarcopenia, accelerates with age, leading to negative health outcomes and poor quality of life. Diet is important to promote health and plays a key role in muscle aging. Plant-based foods have recently received attention as sources of phytochemical components to attenuate loss of muscle mass and strength in older adults. This systematic review and meta-analysis evaluated the benefits of botanical extracts and their phytochemical compounds for muscle health in older adults. Randomized controlled trials were identified via systematic searches of four electronic databases (PubMed, Cochrane Library, Web of Science, and KoreaMed) up to June 2021 and were quality assessed. The results of muscle strength, mass, and physical performance were pooled using a random-effects model. Fourteen studies involving 528 subjects aged between 50 and 80 years met the inclusion criteria. Dietary phytochemicals significantly increased handgrip strength [0.90 kg; 95% confidence interval (CI) 0.26–1.53,p =  0.01] and physical performance (timed up-and-go test: − 0.5 s, 2.73 times; 95% CI − 0.84 to − 0.15,p <  0.01; 30-s chair stand test: 95% CI 0.88–4.59,p <  0.01; 6-min walk test: 29.36 m; 95% CI 14.58–44.13,p <  0.0001) but had no effect on improvement in muscle mass. Publication bias evaluated by funnel plots and Egger’s regression test demonstrated no evidence of substantial publication bias (p >  0.05). The findings of this systematic review and meta-analysis suggest that phytochemicals are a potential nutritional strategy to improve muscle health in older adults.
Cutaneous microbial biofilm formation as an underlying cause of red scrotum syndrome
Authors: Perry Theodore W. 
Published: August 2021

Abstract
Background
Red scrotum syndrome is typically described as well-demarcated erythema of the anterior scrotum accompanied by persistent itching and burning. It is chronic and difficult to treat and contributes to significant psychological distress and reduction in quality of life. The medical literature surrounding the condition is sparse, with the prevalence likely under-recognized and the pathophysiology remaining poorly understood. Formation of a cutaneous microbial biofilm has not been proposed as an underlying etiology. Microbial biofilms can form whenever microorganisms are suspended in fluid on a surface for a prolonged time and are becoming increasingly recognized as important contributors to medical disease (e.g., chronic wounds).

Case presentation
A 26-year-old man abruptly developed well-demarcated erythema of the bilateral scrotum after vaginal secretions were left covering the scrotum overnight. For 14 months, the patient experienced daily scrotal itching and burning while seeking care from multiple physicians and attempting numerous failed therapies. He eventually obtained complete symptomatic relief with the twice daily application of 0.8% menthol powder. Findings in support of a cutaneous microbial biofilm as the underlying etiology include: (1) the condition began following a typical scenario that would facilitate biofilm formation; (2) the demarcation of erythema precisely follows the scrotal hairline, suggesting that hair follicles acted as scaffolding during biofilm formation; (3) despite resolution of symptoms, the scrotal erythema has persisted, unchanged in boundary 15 years after the condition began; and (4) the erythematous skin demonstrates prolonged retention of gentian violet dye in comparison with adjacent unaffected skin, suggesting the presence of dye-avid material on the skin surface.

Conclusion
The probability that microorganisms, under proper conditions, can form biofilm on intact skin is poorly recognized. This case presents a compelling argument for a cutaneous microbial biofilm as the underlying cause of red scrotum syndrome in one patient, and a review of similarities with other reported cases suggests the same etiology is likely responsible for a significant portion of the total disease burden. This etiology may also be a significant contributor to the disease burden of vulvodynia, a condition with many similarities to red scrotum syndrome.
Rural sustainability and food choice: the effect of territorial characteristics on the consumers’ preferences for organic lentils
Authors: Paffarini Chiara , Torquati Biancamaria , Tempesta Tiziano , Venanzi Sonia , Vecchiato Daniel 
Published: August 2021

AbstractThe importance of pulse cultivation and consumption is recognized by the scientific community in terms of human nutrition, food security, biodiversity and a valid substitute for animal protein. In some marginal areas, pulse cultivation represents also a protection against the abandonment of agricultural land, the preservation of traditional landscape and the maintenance of natural environments, besides contributing to the safeguard of traditional gastronomy and culture.This study explores how some characteristics connected with rural sustainability, like the preservation of the traditional rural landscape, production area in a Natura 2000 Site of Community Importance (SCI) and EU quality labels (PDO and PGI), might influence organic consumers’ choice of lentils. Data were collected in the Umbria region (Italy) in 2014 by interviewing 213 consumers’ members of Organic Solidarity Purchase Groups (O-SPGs). The Discrete Choice Experiment methodology was used, and three different models (Multinomial Logit Model (MNL), Mixed Logit Model (RPL) and Endogenous Attribute Attendance (EAA)) were applied to verify the reliability of the estimates. Attribute non-attendance (ANA) behaviour was taken into account. Results reveal that the presence of ANA had an impact on both the relative importance of the estimated attributes and the magnitude of the estimated mean WTP. Therefore, this study suggests that WTP mean estimates should be considered with caution for marketing purposes if ANA is not considered. Looking at pulses, the results help to understand the importance in monetary terms of the relationship between lentil choice and rural sustainability.
Ecological and behavioural risk factors of scrub typhus in central Vietnam: a case-control study
Authors: Tran Hanh Thi Duc , Hattendorf Jan , Do Hung Manh , Hoang Thanh Tien , Hoang Hang Thi Hai , Lam Hoa Ngoc , Huynh Mai Kim , Vu Lan Thi Hoang , Zinsstag Jakob , Paris Daniel Henry , Schelling Esther 
Published: August 2021

AbstractBackgroundThe risk factors for scrub typhus in Vietnam remain unknown. Scrub typhus caused byOrientia tsutsugamushioften presents as an undifferentiated febrile illness and remains under appreciated due to the limited availability of diagnostic tests. This tropical rickettsial illness is increasingly recognized as an important cause of non-malaria acute undifferentiated fever in Asia. This study aimed to investigate behavioural and ecological related risk factors of scrub typhus to prevent this potentially life-threatening disease in Vietnam.MethodsWe conducted a clinical hospital-based active surveillance study, and a retrospective residence-enrolment date-age-matched case–control study in Khanh Hoa province, Vietnam, from August 2018 to March 2020. Clinical examinations, polymerase chain reaction and enzyme-linked immunosorbent assay IgM tests were applied to define cases and controls. All enrolled participants filled out a questionnaire including demographic socio-economic status, personal behaviors/protective equipment, habitat connections, land use, and possible exposure to the vector. Multivariable conditional logistic regression was used to define the scrub typhus associated risk factors.ResultsWe identified 44 confirmed cases and matched them with 152 controls. Among cases and controls, the largest age group was the 41–50 years old and males accounted for 61.4% and 42.8%, respectively. There were similarities in demographic characteristics between the two groups, with the exception of occupation. Several factors were significantly associated with acquisition of scrub typhus, including sitting/laying directly on household floor [adjustedOR(aOR) = 4.9, 95%CI:1.6–15.1,P = 0.006], household with poor sanitation/conditions (aOR = 7.9, 95%CI:1.9–32.9,P = 0.005), workplace environment with risk (aOR = 3.0, 95%CI:1.2–7.6,P = 0.020), always observing mice around home (aOR = 3.7, 95%CI:1.4–9.9,P = 0.008), and use of personal protective equipment in the field (aOR = 0.4, 95%CI:0.1–1.1,P = 0.076).ConclusionsEcological and household hygiene-related factors were more associated with scrub typhus infection, than individual-level exposure activities in the hyper-endemic area. These findings support local education and allow people to protect themselves from scrub typhus, especially in areas with limitations in diagnostic capacity.Graphical abstract
Piloting I-SLEEP: a patient-centered education and empowerment intervention to improve patients’ in-hospital sleep
Authors: Mason Noah R. , Orlov Nicola M. , Anderson Samantha , Byron Maxx , Mozer Christine , Arora Vineet M. 
Published: August 2021

AbstractBackgroundSleep disturbances in hospitalized patients are linked to poor recovery. In preparation for a future randomized controlled trial, this pilot study evaluated the feasibility and acceptability of a multi-component intervention (I-SLEEP) that educates and empowers inpatients to advocate for fewer nighttime disruptions in order to improve sleep during periods of hospitalization.MethodsEligible inpatients received I-SLEEP, which included an educational video, brochure, sleep kit, and three questions patients can ask their team to reduce nighttime disruptions. Following I-SLEEP, inpatients were surveyed on the primary feasibility outcomes of satisfaction with and use of I-SLEEP components. Inpatients were also surveyed regarding empowerment and understanding of intervention materials. Patient charts were reviewed to collect data on nighttime (11 PM–7 AM) vital sign and blood draws disruptions.ResultsNinety percent (n= 26/29) of patients were satisfied with the brochure and 87% (n= 27/31) with the video. Nearly all (95%,n= 36/37) patients felt empowered to ask their providers to minimize nighttime disruptions and 68% (n= 26/37) intended to alter sleep habits post-discharge. Forty-nine percent (n= 18/37) of patients asked an I-SLEEP question. Patients who asked an I-SLEEP question were significantly more likely to experience nights with fewer disruptions due to nighttime vitals (19% vs. 2.1%,p= 0.008).ConclusionThis pilot study found that I-SLEEP was well-accepted and enabled hospitalized patients to advocate for less disrupted sleep. Educating patients to advocate for reducing nighttime disruptions may be a patient-centered, low-cost strategy to improve patients’ care and in-hospital experience. These results suggest that I-SLEEP is ready to be evaluated against routine care in a future randomized controlled trial.Trial registrationClinicalTrials.GovNCT04151251.
Clinical utility of fetal echocardiography: an Egyptian center experience
Authors: Al-Fahham Marwa Moustapha , Gad Nada Ayman , Ramy Ahmed Ramy Mohamed , Habeeb Nevin Mamdouh 
Published: August 2021

Abstract
Background
The impact of early diagnosis of fetal cardiac abnormalities on the postnatal outcome has been controversial in literature. We aimed to evaluate the role of fetal echocardiography (FE) as a diagnostic tool for early detection and proper management of fetal cardiac abnormalities, study the indications of referral and detect the perinatal outcome in our institution.

Results
This is a cross-sectional observational and descriptive study that included one hundred and one singleton pregnant women (101 fetuses) who were referred for FE over a period of one year. Indications for referral and perinatal risk factors were documented. FE and postnatal transthoracic echocardiography were done. Fetal cardiac abnormalities were detected in 46.5% of cases. Congenital heart defects (CHDs) in 34.6%, fetal arrythmias in 9.9%, cardiomyopathy in 2.9% and cardiac mass (Rhabdomyoma) in 1% (combined structural and rhythm abnormalities were observed in two fetuses). Of the CHDs, complex heart lesions were diagnosed in 57.1%, common atrioventricular canal in 28.6% and conotrunchal anomalies in 14.3%. Of the ten cases with fetal arrythmias, five fetuses had tachyarrhythmias, four had ectopics and one fetus had congenital heart block in association with maternal lupus. The indications for referral were abnormal obstetric ultrasound (52.5%), maternal medical illnesses (23.8%), multiple neonatal deaths (13.9%) and positive family history of CHD (10.9%). The number of fetuses with cardiac abnormalities was significantly higher than those without cardiac abnormalities in mothers not exposed to perinatal risk factors (p = 0.009) and was statistically lower in mothers exposed to perinatal risk factors (p = 0.005). FE showed 100% accuracy in diagnosing complex lesions, common atrio-ventricular canals, cono-truncal anomalies, cardiac masses and fetal arrhythmias. It missed two cases of tiny muscular ventricular septal defects and one case of aortic coarctation. Cases of fetal supraventricular tachycardia were successfully treated in-utero.

Conclusions
CHDs exist in fetuses with no underlying perinatal risk factors. FE can accurately diagnose most of the cardiac anomalies though few errors remain challenging (aortic coarctation). It also offers a good chance for successful early life-saving management of some types of fetal arrhythmia.