bluuebunny/crossref_metadata_2025_split huggingface dataset:
Dwarf-shrubs dynamics in Mediterranean high mountain ecosystems
Authors: De Toma Andrea , Carboni Marta , Bazzichetto Manuele , Malavasi Marco , Cutini Maurizio 
Published: October 2021

AbstractQuestionVegetation in the alpine and treeline ecotone faces changes in both climate and land use. Shrub encroachment is considered an effect of these changes, but it’s still unclear how this effect is mediated by environmental heterogeneity. Our goal is to determine which environmental factors shape the fine-scale spatial distribution and temporal trends of alpine dwarf shrub.LocationThree sites in the Central Apennine, Italy.MethodsWe used a comprehensive set of environmental factors across a broad temporal span to model, at a fine-scale, both (1) the current spatial distribution and (2) the change in shrub cover over the past 60 years.ResultsOur results show that dwarf shrubs have generally increased in our study sites over the past 60 years, yet their distribution is strongly shaped by the joint influence of the fine-scale topography, productivity, land use and micro-climate. In particular, shrubs have been locally favored in areas with harsher alpine environmental constraints and stronger resource limitation. Instead, contrary to expectations, at this fine scale, warmer temperatures and the decline in grazing have not favored shrub encroachment.ConclusionDwarf shrubs appear as a stress-tolerant, pioneer vegetation that is currently distributed mainly over areas that are otherwise sparsely vegetated. It appears that shrubs exhibit poor competitive ability to invade grasslands and, though they have increased overall, they remain restricted to the least productive areas. Fine-scale environmental heterogeneity may strongly influence future responses of dwarf shrubs in changing alpine ecosystems.
Modelling sexual violence in male rats: The sexual aggression test (SxAT)
Authors: de Moura Oliveira Vinícius E. , de Jong Trynke R. , Neumann Inga D. 
Published: October 2021

AbstractSexual assault and rape are crimes that impact victims worldwide. Although the psychosocial and eco-evolutionary factors associated with this antisocial behavior have repeatedly been studied, the underlying neurobiological mechanisms are still unknown mainly due to the lack of an appropriate animal model of sexual aggression (SxA). Here, we established a novel paradigm to provoke and subsequently assess SxA in adult male Wistar rats: the sexual aggression test (SxAT). Briefly, male Wistar rats are sexually aroused by a receptive female, which is exchanged by a non-receptive female immediately after the first intromission. This protocol elicits forced mounting (FM) and aggressive behavior (AB) towards the non-receptive female to different degrees, which can be scored. In a series of experiments we can show that SxA behavior is a relatively stable trait in rats and correlates positively with sexual motivation. Rats with innate abnormal anxiety and aggressive behavior also show abnormal SxA behavior. In addition, central infusion of oxytocin moderately inhibits AB, but increases FM. Finally, we identified the agranular insular cortex to be specifically activated by SxA, and inhibition of this region mildly decreased AB during the SxAT.Altogether, the SxAT is a paradigm that can be readily implemented in behavioral laboratories as a valuable tool to find answers regarding the biological mechanisms underlying SxA in humans, as well as social decision-making in general.
Spatial differences in stoichiometry of EGR phosphatase and Microtubule-Associated Stress Protein 1 control root meristem activity during drought stress
Authors: Longkumer Toshisangba , Chen Chih-Yun , Biancucci Marco , Bhaskara Govinal Badiger , Verslues Paul E. 
Published: October 2021

AbstractDuring moderate severity drought and low water potential (ψw) stress, poorly understood signaling mechanisms restrict both meristem cell division and subsequent cell expansion. We found that the Clade E Growth-Regulating 2 (EGR2) protein phosphatase andMicrotubule Associated Stress Protein 1(MASP1) differed in their stoichiometry of expression across the root meristem and had opposing effects on root meristem activity at low ψw. Ectopic MASP1 or EGR expression increased or decreased, respectively, root meristem size and root elongation during low ψwstress. This, along with the ability of phosphomimic MASP1 to overcome EGR suppression of root meristem size and observation that ectopic EGR expression had no effect on unstressed plants, indicated that during low ψwEGR activation and attenuation of MASP1 phosphorylation in their overlapping zone of expression determines root meristem size and activity. Ectopic EGR expression also decreased root cell size at low ψw. Conversely, both theegr1-1egr2-1andegr1-1egr2-1masp1-1mutants had similarly increased root cell size; but, onlyegr1-1egr2-1had increased cell division. These observations demonstrated that EGRs affect meristem activity via MASP1 but affect cell expansion via other mechanisms. Interestingly, EGR2 was highly expressed in the root cortex, a cell type important for growth regulation and environmental response.One Sentence SummarySpatial differences in EGR-MASP1 expression and control of MASP1 phosphorylation adjust root meristem activity to regulate growth during drought stress.The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (www.plantcell.org) is: Paul E. Verslues (paulv@gate.sinica.edu.tw).
Low potential electron generating enzymes serve different functions during aerobic nitrogen fixation in Azotobacter vinelandii
Authors: Alleman Alexander B. , Mus Florence , Peters John W. 
Published: October 2021

AbstractBiological nitrogen fixation requires large amounts of energy in the form of ATP and low potential electrons to overcome the high activation barrier for cleavage of the dinitrogen triple bond. The model aerobic nitrogen-fixing bacteria, Azotobacter vinelandii, generates low potential electrons in the form of reduced ferredoxin (Fd) and flavodoxin (Fld) using two distinct mechanisms via the enzyme complexes Rnf and Fix. Both Rnf and Fix are expressed during nitrogen fixation, and deleting either rnf1 or fix genes has little effect on diazotrophic growth. However, deleting both rnf1 and fix eliminates the ability to grow diazotrophically. Rnf and Fix both use NADH as a source of electrons, but overcoming the energetics of NADH’s endergonic reduction of Fd/Fld is accomplished through different mechanisms. Rnf harnesses free energy from the proton motive force, whereas Fix uses electron bifurcation to effectively couple the endergonic reduction of Fd/Fld to the exergonic reduction of quinone. Different stoichiometries and gene expression analyses indicate specific roles for the two reactions under different conditions. In this work, complementary physiological studies and thermodynamic modeling reveal how Rnf and Fix simultaneously balance redox homeostasis in various conditions. Specifically, the Fix complex is required for efficient growth under low oxygen concentrations, while Rnf sustains homeostasis and delivers sufficient reduced Fd to nitrogenase under standard conditions. This work provides a framework for understanding how the production of low potential electrons sustains robust nitrogen fixation in various conditions.ImportanceThe availability of fixed nitrogen is critical for life in many ecosystems, from hot springs to agriculture. Due to the energy demands of biological nitrogen fixation, organisms must tailor their metabolism during diazotrophic growth to deliver energy requirements to nitrogenase in the form of ATP and low potential electrons. Therefore, a complete understanding of diazotrophic energy metabolism and redox homeostasis is required to understand the impact on ecological communities or to promote crop growth in agriculture through engineered diazotrophs.
Transcriptional differences of lipid-metabolizing enzymes in sebocytes derived from sebaceous glands of the skin and pre-putial glands
Authors: Klas Katharina , Copic Dragan , Direder Martin , Laggner Maria , Prucksamas Patricia S , Gruber Florian , Ankersmit Hendrik Jan , Mildner Michael 
Published: October 2021

AbstractSebaceous glands are adnexal structures, which critically contribute to skin homeostasis and the establishment of a functional epidermal barrier. Sebocytes, the main cell population found within the sebaceous glands, are highly specialized lipid-producing cells. Sebaceous gland-resembling tissue structures are also found in male rodents in form of preputial glands. Similar to sebaceous glands, they are composed of lipid-specialized sebocytes. Due to a lack of adequate organ culture models for skin sebaceous glands and the fact that preputial glands are much larger and easier to handle, previous studies have used preputial glands as a model for skin sebaceous glands. Here, we compared both types of sebocytes, using a single cell RNA sequencing approach, to unravel potential similarities and differences between the two sebocyte populations. In spite of common gene expression patterns due to general lipid-producing properties, we found significant differences in the expression levels of genes encoding enzymes involved in the biogenesis of specialized lipid classes. Specifically, genes critically involved in the mevalonate pathway, including squalene synthase, as well as the sphingolipid salvage pathway, such as ceramide synthase, (acid) sphingomyelinase or acid and alkaline ceramidases, were significantly less expressed by preputial gland sebocytes. Together, our data revealed tissue-specific sebocyte populations, indicating major developmental, functional as well as biosynthetic differences between both glands. The use of preputial glands as surrogate model to study skin sebaceous glands is therefore limited, and major differences between both glands need to be carefully considered before planning an experiment.
Development and characterization of influenza M2 ectodomain and/or HA stalk-based DC-targeting vaccines for different influenza infections
Authors: Olukitibi Titus Abiola , Ao Zhujun , Azizi Hiva , Mahmoudi Mona , Coombs Kevin , Kobasa Darwyn , Kobinger Gary , Yao Xiaojian 
Published: October 2021

AbstractA universal influenza vaccine is required for broad protection against influenza infection. Here, we revealed the efficacy of novel influenza vaccine candidates based on Ebola glycoprotein (EboGP) DC-targeting domain (EΔM) fusion protein technology. We fused influenza hemagglutinin stalk (HAcs) and extracellular matrix protein (M2e) or four copies of M2e (referred to as tetra M2e (tM2e)) with EΔM to generate EΔM-HM2e or EΔM-tM2e, respectively, and revealed that EΔM facilitates DC/macrophage targeting in vitro. In a mouse study, EΔM-HM2e- or EΔM-tM2e-pseudotyped viral particles (PVPs) induced significantly higher titers of anti-HA and/or anti-M2e antibodies. We also developed recombinant vesicular stomatitis virus (rVSV)-EΔM-HM2e and rVSV-EΔM-tM2e vaccines that resulted in rapid and potent induction of HA and/or M2 antibodies in mouse sera and mucosa. Importantly, vaccination protects mice from influenza H1N1 and H3N2 challenges. Taken together, our study suggests that recombinant rVSV-EΔM-HM2e and rVSV-EΔM-tM2e are efficacious and protective universal vaccines against influenza.
Impaired trigeminal control of ingestive behavior in the Prrxl1- / - mouse is associated with a lemniscal-biased orosensory deafferentation
Authors: Resulaj Admir , Wu Jeannette , Hartmann Mitra J. Z. , Feinstein Paul , Zeigler H. Phillip 
Published: October 2021

AbstractAlthough peripheral deafferentation studies have demonstrated a critical role for trigeminal afference in modulating the orosensorimotor control of eating and drinking, the central trigeminal pathways mediating that control, as well as the timescale of control, remain to be elucidated. In rodents, three ascending somatosensory pathways process and relay orofacial mechanosensory input: the lemniscal, paralemniscal, and extralemniscal. Two of these pathways (the lemniscal and extralemniscal) exhibit highly structured topographic representations of the orofacial sensory surface, as exemplified by the one-to-one somatotopic mapping between vibrissae on the animals’ face and barrelettes in brainstem, barreloids in thalamus, and barrels in cortex. Here we use the Prrxl1 knockout mouse model to investigate ingestive behavior deficits associated with disruption of the lemniscal pathway. The Prrxl1 deletion disrupts somatotopic patterning and axonal projections throughout the lemniscal pathway but spares patterning in the extralemniscal nucleus. Our data reveal an imprecise and inefficient ingestive phenotype with deficits that span timescales from milliseconds to months, tightly linking trigeminal input with ingestion, from moment-to-moment consummatory to long term appetitive control. We suggest that ordered assembly of trigeminal sensory information along the lemniscal pathway is critical for the rapid and precise modulation of motor circuits driving eating and drinking action sequences.
Simultaneous mnemonic and predictive representations in the auditory cortex
Authors: Cappotto Drew , Kang HiJee , Li Kongyan , Melloni Lucia , Schnupp Jan , Auksztulewicz Ryszard 
Published: October 2021

AbstractRecent studies have shown that stimulus history can be decoded via the use of broadband sensory impulses to reactivate mnemonic representations. It has also been shown that predictive mechanisms in the auditory system demonstrate similar tonotopic organization of neural activity as that elicited by the perceived stimuli. However, it remains unclear if the mnemonic and predictive information can be decoded from cortical activity simultaneously and from overlapping neural populations. Here, we recorded neural activity using electrocorticography (ECoG) in the auditory cortex of anesthetized rats while exposed to repeated stimulus sequences, where events within the sequence were occasionally replaced with a broadband noise burst or omitted entirely. We show that both stimulus history and predicted stimuli can be decoded from neural responses to broadband impulse at overlapping latencies but linked to largely independent neural populations. We also demonstrate that predictive representations are learned over the course of stimulation at two distinct time scales, reflected in two dissociable time windows of neural activity. These results establish a valuable tool for investigating the neural mechanisms of passive sequence learning, memory encoding, and prediction mechanisms within a single paradigm, and provide novel evidence for learning predictive representations even under anaesthesia.
Identification of factors and outcomes of trust in mobile supply chains
Authors: Ghondaghsaz Nassim , Engesser Sven 
Published: October 2021

PurposeThe main purpose of this paper is the identification of the conceptualization of trust as well as its factors and outcomes in interorganizational relations in mobile supply chains (MSCs) in which multiple stakeholders collaborate.Design/methodology/approachThe authors first used a comprehensive literature review to extract related factors and outcomes of trust. Second, the authors conducted semi-structured interviews in chemical and pharmaceutical companies in Germany. These organizations stand out as leaders in the concept of MSCs and have developed collaborations with various stakeholders.FindingsBased on the results, a conceptual model has been developed that elaborates on the nature of trust and its factors and outcomes for cultivating trustful stakeholder collaboration. The study identifies six factors or approaches for building trust and two outcomes resulting from mutual trust.Practical implicationsThe conceptual model presented in this study can serve as a basis for developing trust in MSCs. Interorganizational collaborations in MSCs are more successful when saturated with trust. The collaboration systems must allow the innovative organizations to create value through the adaptation of advanced technologies without failure.Originality/valueThe study adds to the body of knowledge in building trust in multiple stakeholder collaboration, particularly in innovative organizations which are involved with disruptive technologies.
Ethical marketing strategies: the unique Nash equilibrium
Authors: Krishnamurthy Nagarajan , Swain Biswanath , Ramanathan Jayasankar 
Published: October 2021

Purpose
Can industrial marketers afford to choose unethical strategies? To answer this question, this study aims to use game theory to analyze whether an industrial marketer choosing and implementing an unethical strategy is successful in maximizing her market share across her strategies.

Design/methodology/approach
The competition between two industrial marketers is modeled as a strategic game for the market share of a product that is identical in all attributes except the production process. Each industrial marketer’s objective is to choose to implement either the ethical or the unethical production process to maximize her market share.

Findings
The study finds that both industrial marketers choosing to implement ethical strategies is the unique Nash equilibrium of the game. That is, an industrial marketer choosing to implement an unethical strategy in the production process will be unsuccessful in maximizing her market share when both the industrial marketers are rational.

Research limitations/implications
The study contributes to the literature on industrial marketing ethics, particularly that on product ethics, by showing that industrial marketers gain market share if they choose ethical strategies.

Practical implications
The study has implications for industrial marketing executives, as organizational consumers are increasingly aware of the strategies of industrial marketers. Failure to implement ethical strategies will cause industrial marketers to forgo their best possible market shares.

Originality/value
This study’s novelty lies in using a game theoretic approach to demonstrate the positive implications of ethical strategies for industrial marketers.
Influence of celebrities and online communities on Indian consumers' green clothing involvement and purchase behavior
Authors: Khare Arpita , Sadachar Amrut , Chakraborty Swagata 
Published: October 2021

PurposeThe study examined the role of collective self-esteem (CSE), online communities, green attitudes and the influence of celebrities on green clothing involvement and consequently its impact on green clothing purchase behavior of Indian consumers.Design/methodology/approachA mix of convenience and random sampling was used for data collection via an online survey. The sample (n = 403) comprised consumers having awareness about green clothing. Structural equation modeling (SEM) was used for analysis.FindingsOnline communities, green attitudes and influence of celebrities predicted green clothing involvement and in turn their purchase behavior. CSE had no impact on consumers' green clothing involvement.Practical implicationsThe findings can help green apparel manufacturers and designers to use celebrities and online communities to educate and promote the benefits of green clothing. Social media can be employed to share experiences and engage consumers about green clothing.Originality/valueSince online networking sites are gaining predominance in influencing behavior, the study extends the earlier research on social influence by examining its role along with celebrities and CSE on green apparel involvement and purchase. The study combines celebrities, online communities and collective identity influences (offline and online) in predicting green clothing purchase in India.
Near-shoring versus far-shoring: effects on customer perceived quality and purchase intention
Authors: Colamatteo Annarita , Cassia Fabio , Sansone Marcello 
Published: October 2021

PurposeDriven by the disruptive effects of the Covid-19 pandemic, the ongoing debate about the international location of firms' manufacturing activities has increasingly highlighted the specific benefits and costs of near-shoring versus far-shoring. However, the effects of near-shoring versus far-shoring on customer perceived quality and purchase intention have not been examined. Thus, this study aims to develop a conceptual model and provide new evidence to fill this gap. In particular, the study explores the roles of brand familiarity and corporate social responsibility (CSR) to explain the different levels of perceived quality and purchase intention in relation to near-shoring versus far-shoring.Design/methodology/approachThis study includes two analyses of data collected from a sample of Italian customers. The first analysis consists of a 2 (high/low brand familiarity) × 3 (domestic insourcing, near-shoring, far-shoring) factorial design, and data are assessed via analyses of variance (ANOVA). The second analysis evaluates the suggested model in the two scenarios (near-shoring and far-shoring) via partial least squares–structural equation modelling (PLS-SEM) multigroup analysis.FindingsResults showed that customer perceived quality and purchase intention were significantly higher for near-shoring than for far-shoring, but only when brand familiarity was low. No significant difference was found for participants with a high level of brand familiarity. In addition, the level of a brand's pre-offshoring perceived CSR was negatively related to perceived quality, and this was conceptually justified by the CSR-washing effect. Again, this effect was found only when brand familiarity was low.Research limitations/implicationsThe findings contribute to advancing the current understanding of the multiple effects of the offshoring decision and clarify that near-shoring and far-shoring have different effects for customers with low brand familiarity. The findings also emphasise that the far-shoring decision can elicit the perception of decoupling between the firm's CSR claims and CSR actions, thus decreasing perceived quality.Practical implicationsThis study provides managers with additional inputs to make more informed decisions regarding offshoring. While the post-pandemic scenario seems to favour near-reshoring over far-shoring due to agility considerations, this study also provides additional evidence of the superiority of near-reshoring from the customer's perspective.Originality/valueThis is the first study to examine and prove the differential effects of near-shoring versus far-shoring on the customer's perceptions and behaviours.