bluuebunny/crossref_metadata_2025_split huggingface dataset:
Dual RNA-Seq transcriptome analysis of chicken macrophage-like cells (HD11) infected in vitro with Eimeria tenella
Authors: Sandholt Arnar K. S. , Xu Feifei , Söderlund Robert , Lundén Anna , Troell Karin , Svärd Staffan G. , Wattrang Eva 
Published: February 2021

AbstractThe study aimed to monitor parasite and host gene expression during the early stages of Eimeria tenella infection of chicken cells using dual RNA-Seq analysis. For this, we used chicken macrophage-like cell line HD11 cultures infected in vitro with purified E. tenella sporozoites. Cultures were harvested between 2 and 72 h post-infection and mRNA was extracted and sequenced. Dual RNA-Seq analysis showed clear patterns of altered expression for both parasite and host genes during infection. For example, genes in the chicken immune system showed upregulation early (2–4 h), a strong downregulation of genes across the immune system at 24 h and a repetition of early patterns at 72 h, indicating that invasion by a second generation of parasites was occurring. The observed downregulation may be due to immune self-regulation or to immune evasive mechanisms exerted by E. tenella. Results also suggested pathogen recognition receptors involved in E. tenella innate recognition, MRC2, TLR15 and NLRC5 and showed distinct chemokine and cytokine induction patterns. Moreover, the expression of several functional categories of Eimeria genes, such as rhoptry kinase genes and microneme genes, were also examined, showing distinctive differences which were expressed in sporozoites and merozoites.
Molecular and morphological description of the first Hepatozoon (Apicomplexa: Hepatozoidae) species infecting a neotropical turtle, with an approach to its phylogenetic relationships
Authors: Gutiérrez-Liberato Germán A. , Lotta-Arévalo Ingrid A. , Rodríguez-Almonacid Cristian C. , Vargas-Ramírez Mario , Matta Nubia E. 
Published: February 2021

AbstractHaemogregarines (Adeleorina) have a high prevalence in turtles. Nevertheless, there is only one Hepatozoon species described that infects Testudines so far; it is Hepatozoon fitzsimonsi which infects the African tortoise Kinixys belliana. Colombia harbours a great diversity of chelonians; however, most of them are threatened. It is important to identify and characterize chelonian haemoparasite infections to improve the clinical assessments, treatments and the conservation and reintroduction programs of these animals. To evaluate such infections for the Colombian wood turtle Rhinoclemmys melanosterna, we analysed blood from 70 individuals. By using the morphological characteristics of blood stages as well as molecular information (18S rRNA sequences), here we report a new Hepatozoon species that represents the first report of a hepatozoid species infecting a semi-aquatic continental turtle in the world. Although the isolated lineage clusters within the phylogenetic clades that have morphological species of parasites already determined, their low nodal support makes their position within each group inconclusive. It is important to identify new molecular markers to improve parasite species identification. In-depth research on blood parasites infecting turtles is essential for increasing knowledge that could assess this potential unknown threat, to inform the conservation of turtles and for increasing the state of knowledge on parasites.
Rethinking the course of psychotic disorders: modelling long-term symptom trajectories
Authors: Morgan Craig , Dazzan Paola , Lappin Julia , Heslin Margaret , Donoghue Kim , Fearon Paul , Jones Peter B , Murray Robin M , Doody Gillian A , Reininghaus Ulrich 
Published: February 2021

AbstractBackgroundThe clinical course of psychotic disorders is highly variable. Typically, researchers have captured different course types using broad pre-defined categories. However, whether these adequately capture symptom trajectories of psychotic disorders has not been fully assessed. Using data from AESOP-10, we sought to identify classes of individuals with specific symptom trajectories over a 10-year follow-up using a data-driven approach.MethodAESOP-10 is a follow-up, at 10 years, of 532 incident cases with a first episode of psychosis initially identified in south-east London and Nottingham, UK. Using extensive information on fluctuations in the presence of psychotic symptoms, we fitted growth mixture models to identify latent trajectory classes that accounted for heterogeneity in the patterns of change in psychotic symptoms over time.ResultsWe had sufficient data on psychotic symptoms during the follow-up on 326 incident patients. A four-class quadratic growth mixture model identified four trajectories of psychotic symptoms: (1) remitting-improving (58.5%); (2) late decline (5.6%); (3) late improvement (5.4%); (4) persistent (30.6%). A persistent trajectory, compared with remitting-improving, was associated with gender (more men), black Caribbean ethnicity, low baseline education and high disadvantage, low premorbid IQ, a baseline diagnosis of non-affective psychosis and long DUP. Numbers were small, but there were indications that those with a late decline trajectory more closely resembled those with a persistent trajectory.ConclusionOur current approach to categorising the course of psychotic disorders may misclassify patients. This may confound efforts to elucidate the predictors of long-term course and related biomarkers.
Psychosocial and pharmacological treatments for cannabis use disorder and mental health comorbidities: a narrative review
Authors: Lees Rachel , Hines Lindsey A. , D'Souza Deepak Cyril , Stothart George , Di Forti Marta , Hoch Eva , Freeman Tom P. 
Published: February 2021

AbstractCannabis is the most widely used illicit drug worldwide, and it is estimated that up to 30% of people who use cannabis will develop a cannabis use disorder (CUD). Demand for treatment of CUD is increasing in almost every region of the world and cannabis use is highly comorbid with mental disorders, where sustained use can reduce treatment compliance and increase risk of relapse. In this narrative review, we outline evidence for psychosocial and pharmacological treatment strategies for CUD, both alone and when comorbid with psychosis, anxiety or depression. Psychosocial treatments such as cognitive behavioural therapy, motivational enhancement therapy and contingency management are currently the most effective strategy for treating CUD but are of limited benefit when comorbid with psychosis. Pharmacological treatments targeting the endocannabinoid system have the potential to reduce cannabis withdrawal and cannabis use in CUD. Mental health comorbidities including anxiety, depression and psychosis hinder effective treatment and should be addressed in treatment provision and clinical decision making to reduce the global burden of CUDs. Antipsychotic medication may decrease cannabis use and cannabis craving as well as psychotic symptoms in patients with CUD and psychosis. Targeted treatments for anxiety and depression when comorbid with CUD are feasible.
Twisted Donaldson invariants
Authors: KATO TSUYOSHI , SASAHIRA HIROFUMI , WANG HANG 
Published: February 2021

AbstractFundamental group of a manifold gives a deep effect on its underlying smooth structure. In this paper we introduce a new variant of the Donaldson invariant in Yang–Mills gauge theory from twisting by the Picard group of a 4-manifold in the case when the fundamental group is free abelian. We then generalise it to the general case of fundamental groups by use of the framework of non commutative geometry. We also verify that our invariant distinguishes smooth structures between some homeomorphic 4-manifolds.
N170 reflects visual familiarity and automatic sublexical phonological access in L2 written word processing
Authors: Yum Yen Na , Law Sam-Po 
Published: February 2021

AbstractThe literature has mixed reports on whether the N170, an early visual ERP response to words, signifies orthographic and/or phonological processing, and whether these effects are moderated by script and language expertise. In this study, native Chinese readers, Japanese–Chinese, and Korean–Chinese bilingual readers performed a one-back repetition detection task with single Chinese characters that differed in phonological regularity status. Results using linear mixed effects models showed that Korean–Chinese readers had bilateral N170 response, while native Chinese and Japanese–Chinese groups had left-lateralized N170, with stronger left lateralization in native Chinese than Japanese–Chinese readers. Additionally, across groups, irregular characters had bilateral increase in N170 amplitudes compared to regular characters. These results suggested that visual familiarity to a script rather than orthography-phonology mapping determined the left lateralization of the N170 response, while there was automatic access to sublexical phonology in the N170 time window in native and non-native readers alike.
Android Noahs and embryo Arks: ectogenesis in global catastrophe survival and space colonization
Authors: Edwards Matthew R. 
Published: February 2021

AbstractTo ensure long-term survival of humans and Earth life generally, strategies need to be in place to recolonize Earth after global catastrophes and to colonize exoplanets. In one strategy of space colonization, the physical barriers erected by time and space are circumvented by sending cryopreserved human and animal embryos to exoplanets rather than adult crews. There the embryos would be developed to neonates in artificial uterus (AU) systems. A similar strategy could also be used to repopulate Earth after human extinction events. In this paper, we review the status and future prospects of these embryonic survival strategies. A critical requirement in each scenario is an AU system for complete ectogenesis, i.e. complete development of embryos to neonates outside the natural womb. While such systems do not yet exist, they may soon be developed to afford clinical assistance to infertile women and reproductive choices to prospective parents. In human survival schemes, AU systems would likely first be used to extend conventional survival missions (e.g. subterranean bunkers) by replacing some adult crew members with cryopreserved embryos. For major mass extinctions and all far future events, adult crews would be entirely replaced by embryos and androids. The most advanced missions would feature orbiting embryo spacecraft for Earth recolonization and analogous interstellar spacecraft for colonizing exoplanets. We conclude that an advanced civilization using such an integrated, embryonic approach could eventually colonize distant parts of its home galaxy and potentially the wider universe.
On the Structure of Affect
Authors: Wedderhoff Nadine , Gnambs Timo , Wedderhoff Oliver , Burgard Tanja , Bošnjak Michael 
Published: February 2021

Abstract. The Positive and Negative Affect Schedule (PANAS; Watson et al., 1988 ) is a popular self-report questionnaire that is administered all over the world. Though originally developed to measure two independent factors, different models have been proposed in the literature. Comparisons among alternative models as well as analyses concerning their robustness in cross-national research have left an inconclusive picture. Therefore, the present study evaluates the dimensionality of the PANAS and differences between English and translated versions of the PANAS using a meta-analytic structural equation modeling approach. Correlation matrices from 57 independent samples ( N = 54,043) were pooled across subsamples. For both English and non-English samples, a correlated two-factor model including correlated uniquenesses provided the best fit. However, measurement invariance analyses indicated differences in factor loadings between subsamples. Thus, cross-national application of the PANAS might only be justified if measurement equivalence was explicitly tested for the countries at hand.
New Inventory of Dust Emission Sources in Central Asia and Northwestern China Derived From MODIS Imagery Using Dust Enhancement Technique
Authors: Nobakht Mohamad , Shahgedanova Maria , White Kevin 
Published: February 2021

AbstractThe first inventory of dust emission sources in Central Asia and northwestern China (35–50°N, 50–100°E) derived from the twice daily MODIS imagery from 2003 to 2012 is presented. A high‐resolution (1 km) dust enhancement product was generated to produce maps of dust point sources (DPS), indicating geographical locations of the observed dust emissions, and gridded data sets of dust emission frequencies. About 13,500 DPS were detected over an area of ∼5 × 106 km2, however, their distribution was uneven. The highest frequency of DPS occurred in the northern and eastern Taklimakan, in the Aralkum, and in the regions, which were not widely reported in literature before, the Balkh delta in northern Afghanistan and the pre‐Aral, from the northern Caspian coast to the Betpak‐Dala desert in Kazakhstan. South of the Aral, DPS were mainly associated with fluvial features: drying lakes, dry river beds, alluvial deposits and agricultural activity which is closely linked to water availability in this arid region. In the pre‐Aral and Balkhash‐Junggar regions, land damaged by wildfire was the main source of dust. In China and eastern Kazakhstan dust emissions peaked in spring; in Central Asia and western Kazakhstan—in summer. The Aralkum was active throughout the year with a positive trend in emission frequency over the study period. Locations of DPS did not always correlate with high atmospheric optical depth (AOD) particularly west of the Aral and in the southern Taklimakan where few DPS were detected despite the high AOD values. This was attributed to dust transport from the upwind sources.
Data‐Driven Medium‐Range Weather Prediction With a Resnet Pretrained on Climate Simulations: A New Model for WeatherBench
Authors: Rasp Stephan , Thuerey Nils 
Published: February 2021

AbstractNumerical weather prediction has traditionally been based on the models that discretize the dynamical and physical equations of the atmosphere. Recently, however, the rise of deep learning has created increased interest in purely data‐driven medium‐range weather forecasting with first studies exploring the feasibility of such an approach. To accelerate progress in this area, the WeatherBench benchmark challenge was defined. Here, we train a deep residual convolutional neural network (Resnet) to predict geopotential, temperature and precipitation at 5.625° resolution up to 5 days ahead. To avoid overfitting and improve forecast skill, we pretrain the model using historical climate model output before fine‐tuning on reanalysis data. The resulting forecasts outperform previous submissions to WeatherBench and are comparable in skill to a physical baseline at similar resolution. We also analyze how the neural network creates its predictions and find that, for the case studies analyzed, the model has learned physically reasonable correlations. Finally, we perform scaling experiments to estimate the potential skill of data‐driven approaches at higher resolutions.
Impact of Vegetation Physiology and Phenology on Watershed Hydrology in a Semiarid Watershed in the Pacific Northwest in a Changing Climate
Authors: Zhu Bowen , Huang Maoyi , Cheng Yanyan , Xie Xianhong , Liu Ying , Bisht Gautam , Chen Xingyuan 
Published: February 2021

AbstractChanges in carbon dioxide (CO2) concentration and nitrogen (N) availability can affect land surface processes by regulating physiological (e.g., stomatal opening and closure) and phenological (e.g., leaf area index) responses, which in turn influence terrestrial water cycle dynamics. In this study, we apply the Community Land Model version 5 to investigate how projected environment changes in the 21st century can affect the hydrologic budget in the Upper Columbia‐Priest Rapids watershed, a typical semiarid watershed located in the Pacific Northwest of the United States. Nine numerical experiments were performed to quantifying contributions of climate change, CO2 concentration, and N availability to the watershed hydrologic budget under two Representative Concentration Pathways (RCPs) (i.e., RCP4.5 and RCP8.5). Our results show that compared to its historical value, evapotranspiration (ET) over the watershed increases by 15% and 12% by the end of the century under RCP4.5 and RCP8.5 in responses to changes in meteorological forcing, and further increases by 16% and 11% when elevated CO2 concentrations are accounted for. The effect of N availability on the water balance is insignificant. Such an increase in ET is contributed by the overall increase in leaf area index of vegetation in response to increases in precipitation and CO2 concentration. However, corn‐dominated regions can respond to these changes differently, caused by its increased stomatal resistance under an elevated CO2 concentration with limited N. Our results demonstrate physiological and phenological responses can modulate watershed hydrologic budgets under projected future changes.
Impact of diabetes and periodontal status on life quality
Authors: Desai Radhika , Khobaragade Bhushan , McCracken Giles , Wassall Rebecca , Taylor John J. , Bissett Susan M. , Pumerantz Andrew S. , Preshaw Philip M. 
Published: February 2021

Abstract
Objectives
To investigate impact of periodontal status on quality of life (QoL) in type-1 (T1D) and type-2 (T2D) diabetes patients pre- and post-periodontal treatment using the Well-being Questionnaire 12 (W-BQ12) and Audit of Diabetes-Dependent Quality of Life-19 (ADDQoL-19).

Methods
W-BQ12 and ADDQoL-19 were self-completed by 56 T1D and 77 T2D patients at baseline and by those with periodontitis 3 and 6-months after therapy.

Results
At baseline, T1D patients had significantly higher general W-BQ12 [Median (IQR); 24.00 (20.25–27.75)] and positive well-being scores [8.00 (6.00–9.00)] (indicating better QoL) compared to T2D patients [22.00 (15.50–26.00) and 6.00 (3.50–9.00)], respectively (p < 0.05). Within both groups, general W-BQ12 scores did not differ significantly between patients with periodontal health, gingivitis, or periodontitis (p > 0.05). Significantly higher general W-BQ12 scores were observed in T1D patients at month 3 [28.00 (22.00–29.50)] compared to baseline [22.00 (17.00–24.50)] (p < 0.01), suggesting an initial improvement in QoL post-treatment. ADDQoL-19 identified that diabetes had greatest impact on the domain ‘freedom to eat’, with participants placing most importance on ‘family life’. No significant changes in ADDQoL-19 scores were seen post-treatment (p > 0.05).

Conclusions
Diabetes had impacts upon aspects of life quality in both T1D and T2D patients, though any additional impact based on periodontal status was not observed when using W-BQ12 and ADDQoL-19.
Expanding the role of proteasome homeostasis in Parkinson’s disease: beyond protein breakdown
Authors: Bi Mingxia , Du Xixun , Jiao Qian , Chen Xi , Jiang Hong 
Published: February 2021

AbstractProteasome is the principal hydrolytic machinery responsible for the great majority of protein degradation. The past three decades have testified prominent advances about proteasome involved in almost every aspect of biological processes. Nonetheless, inappropriate increase or decrease in proteasome function is regarded as a causative factor in several diseases. Proteasome abundance and proper assembly need to be precisely controlled. Indeed, various neurodegenerative diseases including Parkinson’s disease (PD) share a common pathological feature, intracellular protein accumulation such as α-synuclein. Proteasome activation may effectively remove aggregates and prevent the neurodegeneration in PD, which provides a potential application for disease-modifying treatment. In this review, we build on the valuable discoveries related to different types of proteolysis by distinct forms of proteasome, and how its regulatory and catalytic particles promote protein elimination. Additionally, we summarize the emerging ideas on the proteasome homeostasis regulation by targeting transcriptional, translational, and post-translational levels. Given the imbalanced proteostasis in PD, the strategies for intensifying proteasomal degradation are advocated as a promising approach for PD clinical intervention.
Diastolic left ventricular function in relation to the retinal microvascular fractal dimension in a Flemish population
Authors: Wei Fang-Fei , Thijs Lutgarde , Melgarejo Jesus D. , Cauwenberghs Nicholas , Zhang Zhen-Yu , Liu Chen , Kuznetsova Tatiana , Struijker-Boudier Harry A. J. , Verhamme Peter , Dong Yu-Gang , Staessen Jan A. 
Published: February 2021

AbstractFractal analysis provides a global assessment of vascular networks (e.g., geometric complexity). We examined the association of diastolic left ventricular (LV) function with the retinal microvascular fractal dimension. A lower fractal dimension signifies a sparser retinal microvascular network. In 628 randomly recruited Flemish individuals (51.3% women; mean age, 50.8 years), we measured diastolic LV function by echocardiography and the retinal microvascular fractal dimension by the box-counting method (Singapore I Vessel Assessment software, version 3.6). The left atrial volume index (LAVI), e′, E/e′ and retinal microvascular fractal dimension averaged (±SD) 24.3 ± 6.2 mL/m2, 10.9 ± 3.6 cm/s, 6.96 ± 2.2, and 1.39 ± 0.05, respectively. The LAVI, E, e′ and E/e′ were associated (P < 0.001) with the retinal microvascular fractal dimension with association sizes (per 1 SD), amounting to −1.49 mL/m2 (95% confidence interval, −1.98 to −1.01), 2.57 cm/s (1.31–3.84), 1.34 cm/s (1.07–1.60), and −0.74 (−0.91 to −0.57), respectively. With adjustments applied for potential covariables, the associations of E peak and E/e′ with the retinal microvascular fractal dimension remained significant (P ≤ 0.020). Over a median follow-up of 5.3 years, 18 deaths occurred. The crude and adjusted hazard ratios expressing the risk of all-cause mortality associated with a 1-SD increment in the retinal microvascular fractal dimension were 0.36 (0.23–0.57; P < 0.001) and 0.57 (0.34–0.96; P = 0.035), respectively. In the general population, a lower retinal microvascular fractal dimension was associated with greater E/e′, a measure of LV filling pressure. These observations can potentially be translated into new strategies for the prevention of diastolic LV dysfunction.