bluuebunny/crossref_metadata_2025_split huggingface dataset:
Artificial Bloodfeeder Glytube: Evaluating Different Types of Membranes and Blood Sources for Feeding Aedes aegypti and Aedes albopictus
Authors: de Almeida Costa Kelion , Garcia Rosário Ingrid N. , Eiras Álvaro E. , da Silva Ivoneide Maria 
Published: February 2021

ABSTRACT
Mosquito colony maintenance in the laboratory is essential for research but presents logistical and ethical problems with the use of live animals for bloodfeeding. The Glytube is an artificial bloodfeeding system for mosquitoes that uses Parafilm-M® membrane and human blood to feed Aedes aegypti. This study evaluated the efficiency of Glytube with different types of membranes and chicken blood to feed Ae. aegypti and Ae. albopictus. We evaluated 2 artificial (thread seal tape [TST], Parafilm-M) and 2 natural membranes (pork, sheep intestine). The results for Ae. aegypti suggest that TST was the best membrane because it presented a high percentage of fed females (63%), a high average number of eggs per female (54.65), and an egg viability rate significantly similar to control (mouse). For Ae. albopictus, there was no significant difference between the membranes and the control; however, the use of TST is suggested due to the low cost and easy manipulation. The treatments that used chicken blood did not present significant differences in the egg viability when compared with the control. The Glytube functionality can be increased by replacing the Parafilm-M membrane by TST and human to chicken blood.
Lives de festas nos tempos da COVID-19: arranjos, vínculos e performances
Authors: Silva Patrícia , Lima Danillo Mota , Couto Edvaldo Souza 
Published: February 2021

Com a pandemia da Covid-19 fomos estimulados por governos e profissionais de saúde a ficar em casa, em isolamento físico. Para as pessoas conectadas o isolamento físico não significa isolamento social. Nossas experiências ciberculturais são repletas de redes sociotécnicas que reconstroem e impulsionam a nossa vida social, sobretudo no contexto da pandemia, por meio, por exemplo, do fenômeno das lives. Nesse contexto, o objetivo do artigo é analisar arranjos, vínculos e narrativas gerados a partir das performances humanas e não-humanas em uma live de uma festa virtual. O argumento central é que a live é uma pedagogia cultural que nos orienta a festejar a vida, cuidar do corpo, explorar outras possibilidades para a paquera e a sexualidade online, ao mesmo tempo em que continuamos a defender nossas pautas políticas e ativistas. O método usado foi o da pesquisa pós-qualitativa, por meio da estratégia da observação participante. Concluímos que as lives rapidamente se popularizaram como redes sociotécnicas dinâmicas, repletas de pedagogias culturais que nos ajudam a organizar a vida social em meio ao isolamento físico e que a live da festa da resistência, com suas performances celebradas, mostra que reinventamos o social, agora na esfera do online, promovendo diversos hibridismos.
Online Personalised Training in Memory Strategies for Everyday (OPTIMiSE) Program for Older Adults with Cognitive Concerns: Pilot Study Protocol
Authors: Pike Kerryn E. , Moller Carl I. , Bryant Christina , Farrow Maree , Dao Duy P. , Ellis Kathryn A. 
Published: February 2021

Memory interventions for older adults with cognitive concerns result in improved memory performance and maintenance of cognitive health. These programs are typically delivered face-to-face, which is resource intensive and creates access barriers, particularly for those with reduced mobility, limited transportation, and living in rural or remote areas. The COVID-19 pandemic has created an additional access barrier, given the increased risk this disease poses to older adults. Internet-based interventions seek to overcome these barriers. This paper describes the protocol of a pilot study that aims to evaluate the feasibility, acceptability, and efficacy of one such internet-based intervention: the Online Personalised Training in Memory Strategies for Everyday (OPTIMiSE) program. OPTIMiSE focuses on improving knowledge regarding memory and providing training in effective memory strategies for everyday life. The pilot study described in this protocol will be a single-arm pre-post study of 8 weeks duration, with a single maintenance session 3 months post-intervention. Participants will be Australian adults aged ≥60 years reporting cognitive changes compared with 10 years ago. Primary outcome measures will address feasibility, acceptability, and efficacy. Secondary outcome measures assessing sense of community and self-efficacy will be administered at the 8-week and 3-month timepoints. Data collection will conclude mid-2021, and results will be presented in a subsequent publication. Translation of memory interventions to internet-based delivery has the potential to remove many access barriers for older adults; however, the acceptability and feasibility of this modality needs investigation. OPTIMiSE is the initial step in what could be an important program enabling access to an evidence-based memory intervention for older adults worldwide. Trial registration: Australian New Zealand Clinical Trials Registry (ANZCTR), ACTRN12620000979954
Amyloid-β Oligomers Induce Only Mild Changes to Inhibitory Bouton Dynamics
Authors: Ruiter Marvin , Lützkendorf Christine , Liang Jian , Wierenga Corette J. 
Published: February 2021

The amyloid-β protein precursor is highly expressed in a subset of inhibitory neuron in the hippocampus, and inhibitory neurons have been suggested to play an important role in early Alzheimer’s disease plaque load. Here we investigated bouton dynamics in axons of hippocampal interneurons in two independent amyloidosis models. Short-term (24 h) amyloid-β (Aβ)-oligomer application to organotypic hippocampal slices slightly increased inhibitory bouton dynamics, but bouton density and dynamics were unchanged in hippocampus slices of young-adult App NL - F - G-mice, in which Aβ levels are chronically elevated. These results indicate that loss or defective adaptation of inhibitory synapses are not a major contribution to Aβ-induced hyperexcitability.
Betahydroxybutyrate Consumption in Autopsy Brain Tissue from Alzheimer’s Disease Subjects
Authors: Swerdlow Russell H. , de Leon Mony J. , Marcus David L. 
Published: February 2021

Background: Alzheimer’s disease (AD) features perturbed brain glucose utilization, which could contribute to brain bioenergetic failure. This led some to consider using ketone bodies to enhance AD brain bioenergetics and treat AD. Objective: We evaluated the rate at which brain homogenates from persons with Alzheimer’s disease (AD) metabolize D-β-hydroxybutyrate (BHB). Methods: We homogenized pieces of temporal cortex from frozen autopsy brains obtained from recently deceased AD subjects ( n = 4), and age-matched subjects that did not have clinical AD ( n = 3). Measuring the rate of CO2 production that followed the introduction of radiolabeled BHB to the homogenates yielded a BHB utilization rate. Results: Compared to the control homogenates, the BHB-supported CO2 production rate was 66%lower in the AD homogenates ( p < 0.05). Conclusions: AD brains can utilize BHB, albeit less robustly than control brains. In conjunction with a previous study that demonstrated reduced glucose utilization in AD brain homogenates, our BHB data provide further evidence of AD brain mitochondrial dysfunction or altered mitochondrial biology.
EMBRYONIC HEART RATE: A PROGNOSTIC FACTOR OF FIRST TRIMESTER PREGNANCY OUTCOME
Authors: Choudhary Chetana , Rajoria Lata , Kuntal Chelsae , Hemani Sunita , Bansal Aditi , Bala Suman 
Published: February 2021

Introduction: The earliest proof of a viable pregnancy is obtained when cardiac activity of the embryo can be observed. Transvaginal sonography can accurately demonstrate embryonic heart rate at 6 weeks of gestational age by using M-mode transvaginal sonography. Slow embryonic heart rate at 6-7 weeks of gestational age is associated with high rate of first trimester pregnancy loss.
AIM: The study was aimed to evaluate the role of embryonic heart rate (EHR) of early pregnancies as predictive factor of adverse outcome at end of first trimester of pregnancy.
Methods: This observational study was conducted in the Department of Obstetrics and Gynecology Zenana Hospital, SMS Medical College, Jaipur. It included 300 pregnant women between 6 weeks to 9 weeks of gestational age attending antenatal OPD. Embryonic heart rate was measured by transvaginal sonography. Embryonic heart rate was classified as slow, if it was fewer than 110 beats/ mint or outcome was measured as occurance of spontaneous pregnancy loss prior to 12 weeks.
Results: Out of 300 cases, 290 (96.67%) had embryonic heart rate ?100 beats per minutes and 10(3.33%) had embryonic heart rate <100 beats per minutes. Out of these 290, (Embryonic heart rate ?100 beats per minutes), 284 (97.93%) had good prognosis and 6 (2.07%) had abortion. In 10 women (Embryonic heart rate <100 beats per minutes), 3 (30.00%) had good prognosis and 7 (70.00%) had abortion. This observation was statistically significant.
Conclusion: Slow embryonic heart rate on ultrasonography reduced the success of pregnancy and may lead to abortion.
Keywords: Embryonic heart rate, Ultrasonography, Early pregnancy loss
FETO -MATERNAL OUTCOME IN POST DATED PREGNANCY
Authors: Maheshwari Shruti , Rajoria Lata , Hemani Sunita , Kuntal Chelsea 
Published: February 2021

Background: The risk to the fetus increases after term (>42 weeks) of gestation mainly due to increasing fetal weight, decline in placental function, oligohydraminos which increases the chances of cord compression, and me conium aspiration.Perinatal mortality after 42 weeks is twice as compared to the perinatal mortality at 40 weeks and by 44 weeks the rate is increased up to threefold. In cases of prolonged pregnancy, fetus is more at risk of hypoxia during labor than a fetus at term
Methods: This cross-sectional observational study of fetomaternal outcome in post dated pregnancy(Women beyond 40 weeks of gestation) was carried in department of obstetrics and gynecology SMS medical college jaipur in 40 patients from October 2019 to January 2020.
Results: Majority patients required cesarean section (52%).Among patients who were given induction 48% had successful induction, maximum were induced by dinoprostone gel (50%).15% of babies delivered required NICU admission.
Conclusion: The present study, we conclude that, the post dated pregnancy can be considered as a high risk factor from the point of view of maternal and fetal outcome as there is more fetal and maternal morbidity
Keywords: Morbidity, Fetomaternal, NICU
PENGARUH NESTING TERHADAP PERUBAHAN FISIOLOGIS BAYI PREMATUR DI RUANG PERINATOLOGI RSUD DR. M. YUNUS BENGKULU
Authors: Eliyanti Yeni , Noeraini Nasaratri Hasta 
Published: February 2021

Latar Belakang : Prematuritas merupakan penyebab kematian kedua pada balita setelah pneumonia dan merupakan penyebab utama kematian neonatal. Tiga puluh lima persen kematian neonatal di dunia disebabkan oleh komplikasi kelahiran prematur. Salah satu bentuk intervensi keperawatan selain metode kanguru yang dapat dilakukan pada BBLR adalah nesting. Nesting digunakan untuk memberikan posisi yang tepat pada neonatus. Intervensi keperawatan tersebut menggunakan alat berbentuk seperti kondisi rahim ibu yang terbuat dari bahan yang halus yang berisi potongan kain seperti dacron. Panjang alat ini sekitar 121-132 cm dan dapat disesuaikan dengan panjang tubuh bayi. Alat ini diletakkan sebagai pelindung posisi bayi, sehingga berada dalam posisi ekstensi dan menjaga perubahan posisi bayi yang diakibatkan karena gravitasi.
Masalah penelitian : masih banyaknya bayi premature yang keadaan fisiologisnya tidak stabil di Ruang Perinatologi RSUD dr. M. Yunus Bengkulu.
Tujuan: Mengetahui pengaruh nesting terhadap perubahan fisiologis bayi premature di Ruang perinatology RSUD DR. M. Yunus Bengkulu.
Metode Penelitian : Rancangan penelitian yang digunakan adalah quasi eksperiment dengan rancangan non equidment control group design dengan satu kelompok intervensi dan satu kelompok kontrol.
Hasil Penelitian : Hasil penelitian di dapatkan Rata-rata saturasi oksigen pada kelompok kontrol adalah 96,22 sedangkan pada kelompok intervensi didapatkan rata-rata saturasi oksigen adalah 98,17 di Ruang Perinatologi RSUD dr. M. Yunus Bengkulu. Rata-rata frekuensi nafas pada kelompok kontrol adalah 85,44 edangkan pada kelompok intervensi didapatkan rata-rata frekuensi nafas adalah 97,61 di Ruang Perinatologi RSUD dr. M. Yunus Bengkulu. Rata-rata frekuensi nadi pada kelompok kontrol adalah 97,44 sedangkan pada kelompok intervensi didapatkan rata-rata frekuensi nadi adalah 110,50 di Ruang Perinatologi RSUD dr. M. Yunus Bengkulu. Ada pengaruh nesting terhadap saturasi oksigen pada bayi prematur di Ruang Perinatologi RSUD DR. M. Yunus Bengkulu dengan p value 0,007. Ada pengaruh nesting terhadap frekuensi nafas pada bayi prematur di Ruang Perinatologi RSUD DR. M. Yunus Bengkulu dengan p value 0,003 dan ada pengaruh nesting terhadap frekuensi nadi pada bayi prematur di Ruang Perinatologi RSUD DR. M. Yunus Bengkulu dengan p value 0,047.
Simpulan : Ada pengaruh nesting terhadap saturasi oksigen pada bayi prematur di Ruang Perinatologi RSUD DR. M. Yunus Bengkulu dengan p value 0,007. Ada pengaruh nesting terhadap frekuensi nafas pada bayi prematur di Ruang Perinatologi RSUD DR. M. Yunus Bengkulu dengan p value 0,003 dan ada pengaruh nesting terhadap frekuensi nadi pada bayi prematur di Ruang Perinatologi RSUD DR. M. Yunus Bengkulu dengan p value 0,047.
DIVERSITAS GOLONGAN DARAH SISTEM ABO BERDASARKAN RIWAYAT DEMAM BERDARAH DENGUE PADA MAHASISWA DI PADANG HARAPAN KOTA BENGKULU
Authors: Irawan Putra Adi , Krisyanella Krisyanella 
Published: February 2021

Abstrak
Latar Belakang: Diversitas golongan darah berdasarkan penyakit tertentu seperti DBD hingga kini masih menjadi perhatian oleh sejumlah peneliti. Demam Berdarah Dengue hingga kini masih menjadi masalah kesehatan nasional Indonesia merupakan salah satu Negara yang memiliki kasus DBD yang tergolong masih cukup tinggi. Provinsi Bengkulu sebelumnya berada pada urutan ke 26 dengan angka kesakitan 17,53 pada tahun 2017, naik menjadi urutan ke 3 dengan angka kesakitan 72,28 per 100.000 penduduk pada tahun 2018. Data dua (2) tahun terakhir menunjukkan bahwa Kota Bengkulu merupakan salah satu wilayah dengan kasus DBD terbanyak. Data diversitas golongan darah sistem ABO berdasarkan riwayat penyakit DBD di Padang Harapan Kota Bengkulu masih sangat kurang. Belum ada data pasti baik di Dinas terkait maupun Rumah Sakit Daerah dan Provinsi, sementara kasus Demam Berdarah kerap terjadi dengan angka yang fluktutif setiap tahunya
Metode: Penelitian ini merupakan non-eksperimen dengan desain observasional analitik menggunakan pendekatan cross-sectional. Responden adalah mahasiswa di Padang Harapan Kota Bengkulu dipilih berdasarkan kriteria inklusi dan eksklusi sejumlah 391 orang menggunakan teknik purporsive sampling dengan rentang usia 17-25 Tahun. Penelitian berlangsung selama Maret-November 2020. Data golongan darah dan riwayat DBD responden dikumpulkan menggunakan google form dan dikonfirmasi dengan pemeriksaan laboratorium.
Hasil: Diversitas golongan darah mahasiswa sistim ABO didominasi oleh golongan darah A (32,5 %), O (31,2%), B (25,6%), dan AB (10,7%). Sebesar 9,7 % mahasiswa di Padang Harapan (n=391) pernah mengalami DBD. Diversitas golongan darah mahasiswa yang pernah mengalami DBD (n=38) didominasi oleh Golongan darah A (44,7%), B (28,9%), O (18,4%), dan AB (7,9%).
Kesimpulan: Terdapat hubugan bermakna (P=0,032) antara golongan darah dengan riwayat DBD.
Activation of Human Osteoblasts via Different Bovine Bone Substitute Materials With and Without Injectable Platelet Rich Fibrin in vitro
Authors: Kyyak Solomiya , Blatt Sebastian , Schiegnitz Eik , Heimes Diana , Staedt Henning , Thiem Daniel G. E. , Sagheb Keyvan , Al-Nawas Bilal , Kämmerer Peer W. 
Published: February 2021

IntroductionThe aim of the in vitro study was to compare the effect of four bovine bone substitute materials (XBSM) with and without injectable platelet-reach fibrin for viability and metabolic activity of human osteoblasts (HOB) as well as expression of alkaline phosphatase (ALP), bone morphogenetic protein 2 (BMP-2), and osteonectin (OCN).Materials and MethodsCerabone® (CB), Bio-Oss® (BO), Creos Xenogain® (CX) and MinerOss® X (MO) ± i-PRF were incubated with HOB. At day 3, 7, and 10, cell viability and metabolic activity as well as expression of ALP, OCN, and BMP-2, was examined.ResultsFor non-i-PRF groups, the highest values concerning viability were seen for CB at all time points. Pre-treatment with i-PRF increased viability in all groups with the highest values for CB-i-PRF after 3 and 7 and for CX-i-PRF after 10 days. For metabolic activity, the highest rate among non-i-PRF groups was seen for MO at day 3 and for CB at day 7 and 10. Here, i-PRF groups showed higher values than non-i-PRF groups (highest values: CB + i-PRF) at all time points. There was no difference in ALP-expression between groups. For OCN expression in non-i-PRF groups, CB showed the highest values after day 3, CX after day 7 and 10. Among i-PRF-groups, the highest values were seen for CX + i-PRF. At day 3, the highest BMP-2 expression was observed for CX. Here, for i-PRF groups, the highest increase was seen for CX + i-PRF at day 3. At day 7 and 10, there was no significant difference among groups.ConclusionXBSM sintered under high temperature showed increased HOB viability and metabolic activity through the whole period when compared to XBSM manufactured at lower temperatures. Overall, the combination of XBSM with i-PRF improved all cellular parameters, ALP and BMP-2 expression at earlier stages as well as OCN expression at later stages.
Rationality of Time-Dependent Antimicrobial Use in Intensive Care Units in China: A Nationwide Cross-Sectional Survey
Authors: Liu Jiao , Zhang Sheng , Huang Sisi , Chen Yizhu , Zhang Lidi , Du Hangxiang , Wang Tao , Liu Yongan , Xu Yan , Chen Dechang 
Published: February 2021

Background: Extended/continuous infusion and therapeutic drug monitoring (TDM) of time-dependent antimicrobials are recommended for optimizing drug exposure for patients in intensive care units (ICUs), although practical application of these measures remains uncertain. We surveyed current practices in infusion and monitoring of commonly prescribed time-dependent antimicrobials in ICUs across China.Methods: From December 2019 to January 2020, we sent online questionnaires about various aspects of infusion and monitoring of time-dependent antimicrobials to intensivists across China. Responses from clinicians were matched with their professional titles using the Sankey diagram. Univariate and multivariate logistic regression analyses were performed to find factors associated with TDM.Results: A total of 3,687 ICU specialists from 31 provincial administrative regions of China responded to our questionnaires. Antibiotic stewardship (ABS) teams were available in hospitals as reported by 3,243 (88.0%) intensivists, including 1,308 (35.5%) who were ABS team members. Although most intensivists (3,490, 94.7%) were acquainted with the concept of prolonged/continuous infusion, nearly half of them (1,634, 44.3%) commonly administered β-lactam antibiotics intermittently. Nearly two-thirds of the respondents reported that their hospitals could not perform TDM. Our multivariable logistic regression analysis revealed that at the hospital level, knowledge of drug sample timing and attitude toward monitoring treatment effects, and drug trough or peak concentration influenced the decision to conduct TDM.Conclusions: We found great variability in prescribing practices, from drug administration to TDM, for several time-dependent antibiotics commonly used for patients with severe infections. Further studies are necessary to effectively evaluate strategies to promote consistent prescribing behavior.
Levofloxacin Detection Using l-Cysteine Capped MgS Quantum Dots via the Photoinduced Electron Transfer Process
Authors: Hashmi S. Z. H. , Dhiman Tarun Kumar , Chaudhary Navneet , Singh Avinash Kumar , Kumar Rahul , Sharma Jai Gopal , Kumar Anil , Solanki Pratima R. 
Published: February 2021

Antibiotics resistance is becoming one of the biggest problems of the 21st century. The prior detection of antibiotics resistance can help human beings in better treatment of diseases. Here, we have used l-Cysteine capped magnesium sulfide quantum dots (L-Cyst-MgS QDs) to detect Levofloxacin antibiotic. L-Cyst-MgS QDs were synthesized using the hydrothermal method. Transmission electron microscopy study showed monodispersed L-Cyst-MgS QDs of 2–4 nm in size. Energy dispersive x-ray photoemission spectroscopy study confirmed the elemental composition of the L-Cyst-MgS QDs without any impurity. UV-vis absorption study showed a peak centered around 340 nm. The photoluminescence study exhibited the maximum peak at 410 nm for 340 nm of excitation wavelength. L-Cyst-MgS QDs were studied with thirteen antibiotics, namely Thiamphenicol, Gentamicin, Erythromycin, Ofloxacin, Ampicillin, Ciprofloxacin, Tetracycline, Chloramphenicol, Florfenicol, Amoxicillin, Moxifloxacin, Norfloxacin, and Levofloxacin. Among these, Levofloxacin showed the most significant change in the peaks’ intensity and was further used for the interaction study. In the interaction study, the peak corresponding to MgS showed a continuous decrease, while the peak corresponding to Levofloxacin showed an increase with the increased concentrations (0–100 μg/ml) of Levofloxacin. Linear behavior was obtained in the range of 1–90 μg/ml. FT-IR study confirmed the interaction of the Levofloxacin with L-Cyst-MgS QDs. The Time-resolved fluorescence spectroscopy showed identical lifetime for both the samples and no spectral overlap confirm the FRET free system. The underlying mechanism is explained based on the electron transfer from the conduction band of the L-Cyst-MgS QDs to the HOMO of Levofloxacin. The limit of detection was found to be 0.21 μg/ml.