bluuebunny/crossref_metadata_2025_split huggingface dataset:
Tumor-Stroma Co-Cultures Modify the Invasive Properties of Human Pancreatic Adenocarcinoma Cells Through Different Patterns of Cytokine Secretion and Depending on the Type of Cell Lines.
Authors: Prieto-García Elena , Díaz-García C. Vanesa , Agudo-López Alba , Pardo-Marqués Virginia , García-Consuegra Inés , Asensio-Peña Sara , Alonso-Riaño Marina , Pérez Carlos , Gómez Carlos , Adeva Jorge , Paz-Ares Luis , López-Martín José A. , Agulló-Ortuño María Teresa 
Published: January 2021

Abstract
Background: Pancreatic ductal adenocarcinoma (PDAC) is among the most lethal cancer worldwide. One key feature of PDAC is a dense desmoplastic reaction that has become recognized to play important roles in tumor growth, metastasis, and therapeutic resistance. We aim to study tumor-stromal interactions in an in vitro co-culture model between human PDAC cells and fibroblasts.Methods: Capan-1 or PL-45 human pancreatic tumor cell lines and LC5 fibroblasts were grown in direct or indirect co-cultures, and their relationship regarding migratory and invasive properties were studied by wound-healing and transwell migration assays. Confocal immunofluorescence, ELISA, and western blotting were used to evaluate the expression of activation markers. Cytokines arrays were performed to identify secretome profiles associated to migratory and invasive properties of tumor cells. Extracellular vesicles production was examined by ELISA and transmission electron microscopy. Results: Co-cultures conditions increased FGF-7 secretion and α-SMA expression, characterizing fibroblasts activation; and decreased epithelial marker E-cadherin in tumor cells, suggesting that they suffer epithelial-to-mesenchymal transition. The expression pattern of cytokine secretion differed under co-culture conditions versus monocultures, with GM-CSF, GRO a/b/g, GRO-α, IL-6, IL-8, MCP-1, and RANTES being the most affected. A greater number of exosomes with higher amount of proteins was quantified in co-cultures assays. Interestingly, tumor cells and fibroblasts migrate as a package, with tumor cells in the middle surrounded by fibroblasts, and maximizing the contact between cell to cell. Conclusions: We show different migratory properties in tumor and stromal cells, and we draw a different mechanism for tumor spread through a cooperative migration between tumor cells and activated fibroblasts. Furthermore, we found that the level of IL-6 mainly, in addition to other cytokines, change significantly in co-cultures conditions with respect to monocultures, and this could affect the invasive and migratory capacities of cells. Targeting the interaction between tumor cells and tumor microenvironment might represent a novel therapeutic approach to advance pancreatic carcinoma.
Preneoplastic Lesions Fimbria Early Diagnosis Markers Underlying Timeline Mechanisms at The Origin of Ovarian Cancer in BRAC1/2 Patients: Case Reports Based on Proteogenomic Study
Authors: Wisztorski Maxence , Saudemont Philippe , Aboulouard Soulaimane , Cardon Tristan , Narducci Fabrice , Robin Yves-Marie , Lemaire Anne-Sophie , Bertin Delphine , Kobeissy Firas , Leblanc Eric , Fournier Isabelle , Salzet Michel 
Published: January 2021

Abstract
Background : BRCA 1 or 2 mutations have been known to be drivers of high-grade serous ovarian carcinomas (HGSC). As no efficient screening is currently available, a bilateral prophylactic salpingo-oophorectomy is the recommended procedure to avoid this often lethal carcinomas. However, a proportion of BRCA mutation carriers refuse to undergo this procedure due to significant impacts on quality and quantity of life, especially if a hormone replacement therapy is contra-indicated. Such decision expose them to developing a pelvic serous carcinoma. A better undersanding of high-grade serous cancer pathogenesis, rather stemming from fallopian tubes than from ovary itself, makes interesting a two-step prophylactic strategy in which tubes are removed as soon as possible, followed by oophorectomies at the time of menopause. Thus women are protected against HGSC with no immediate need of an HRT. Following this idea and considering the concept of junctional “hot spots “ (called mesothelo-müllerian junctions) for the development of HGSC, we suggested as first step to perform a bilateral prophylactic radical fimbriectomy rather than salpingectomy with a delayed oophorectomy (RF/DO). This operation was tested in a national phase 2 trial from 2011-2014 (NCT01608074). Radical Fimbriectomy (RF) consists of the surgical removal of both fallopian tubes along with a tiny part of the adherent ovary to suppress the tubal source of possible dysplastic cells from which can stem a high-grade invasive tumor, through stepwise cellular and genomic altereations ranging from p53 signatures to serous tubal intraepithelial carcoinoma (STIC), just before genuine invasive HGSC, through intermediate aspects (STIL) and (TILT). Methods : In this context, we carried out an in-depth proteomics analysis of these “pre-invasive ” epithelial lesions based on spatially resolved proteomic/ and bioinformatics systems biology platforms platform guided by immunohistochemistry (IHC) technique. Results : Specific markers related to each preneoplastic lesions and in particular from normal to p53 signatures were evaluated. We identified EIF3B, MOB1B and Emilin2, CAVIN1 as bad prognosis markers of P53 signature whereas CAVIN2, SPTANi, FBLN5 as good prognosis markers. Among the novel markers identified, mutated proteins as well as hidden proteins translated from alternative ORF Proteome have been sequenced and characterized. Conlusion : In summary, our results represent a novel pioneer approach to identify the unique under investigated markers that can characterize the molecular mechanism occurring in the preneoplastic lesion in fimbria at the origin of the HGSC.
Novel Hybrid Hypercrosslinked Polymer Assisted Highly Efficient Thermal Energy Storage
Authors: Liu Changhui , Zhang Jiahao , Liu Jian , Tan Zengyi , Cao Yuqi , Li Xia , Rao Zhonghao 
Published: January 2021

Abstract
Though a range of methods has been dedicated to solving the leakage problems, a versatile method which can meet with the organic and inorganic phase change materials (PCMs) simultaneously still have not been seen. In this work, an organic/inorganic hybrid Silioxyl functional group containing polymer was synthesized and applied to encapsulated PCMs. Owing to the mild hypercrosslinking reaction conditions, which only requires the addition of catalytic amount of aqueous alkali solution, renders it to be tolerance towards both organic and inorganic PCMs. More importantly, the homogeneous state of the initiate state of the mixture allowed the ultimate PCMs encapsulation rate and the uniformly blending of the third nano-additives with the aim of thermal conductivity enhancement. Further study reveals that the presence of this hybrid polymers endows a unique self-cleaning property to the obtained phase change composite that it displays a good hydrophobicity towards water droplets. More importantly, this novel PCMs encapsulation protocol shows a good tolerance with varies of nanoparticles, including carbon based nanomaterial, metal oxide nanoparticles and inorganic oxide nanoparticles, which enables an utmost 600% thermal conductivity enhancement and 93.7% light-to-thermal conversion efficiency with a latent heat of 180 J/g without the observation of any leakage.
An ionising radiation-induced specific transcriptional signature of inflammation-associated genes in human whole blood: a pilot study
Authors: Cruz-Garcia Lourdes , Badie Christophe , Anbalagan Selvakumar , Moquet Jayne , Gothard Lone , O’Brien Grainne , Somaiah Navita , Ainsbury Elizabeth A. 
Published: January 2021

Abstract
This communication reports the identification of a new panel of transcriptional changes in inflammation-associated genes observed in response to ionising radiation received by radiotherapy patients. Peripheral blood samples were taken with ethical approval and informed consent from a total of 20 patients undergoing external beam radiotherapy for breast, lung, gastrointestinal or genitourinary tumours. Nanostring nCounter analysis of transcriptional changes was carried out in samples prior and 24 hours post-delivery of the 1st radiotherapy fraction, just prior to the 5th or 6th fraction, and just before the last fraction. Statistical analysis with BRB Array Tools, GLM MANOVA and nSolver, revealed a radiation responsive panel of genes which varied by patient group (type of cancer) and with time since exposure (as an analogue for dose received), which may be useful as a biomarker of radiation response. Further validation in a wider group of patients is ongoing, together with work towards a full understanding of patient specific responses in support of personalised approaches to radiation medicine.
Food related taboos and misconceptions during pregnancy among rural communities of Illu Aba Bor Zone, Southwest Ethiopia.
Authors: Tsegaye Dereje , Tamiru Dessalegn , Belachew Tefera 
Published: January 2021

Abstract
Background: Poor maternal nutrition adversely affects pregnancy and birth outcomes. In many societies, pregnant women have food taboos and misconceptions which consequently results in the depletion of vital nutrients. These cultural malpractices and beliefs can influence dietary intake of pregnant women which subsequently affects the birth outcome. This study aimed at exploring the extent of food taboos and misconceptions during pregnancy in rural communities of Illu Aba Bor Zone, Southwest Ethiopia. Methods: A qualitative study was conducted using in-depth interviews of key informants and focus group discussions among purposefully selected pregnant women and their husbands, elderly people, health workers and health extension workers. Data were transcribed verbatim, thematized; color coded and analyzed manually using the thematic framework method.Result: Thorough reading and review of the transcripts generated three major themes. The first theme was belief and practice of taboos related to the intake of certain food items during pregnancy. Pregnant women, their husbands and mothers- in- law believed that certain foods should be avoided during pregnancy. The second theme was foods that were held as taboo and the reason attached to the food taboos. The most common food items held as taboo were related to the consumption of vegetables like cabbage, pumpkin, milk and milk products, sugar cane, fruit like banana and avocado and egg and the main reasons to avoid these foods were a belief that it can be plastered on the fetal head, making fatty baby which is difficult for delivery. The third theme was the reasons underlying adherence to food taboos which is deeply embedded in the personal believes and attitudes of the pregnant women, who were nested within the influence of the social environment surrounding them and the traditional beliefs and values of the society in general. Conclusions: The results showed a widespread practice of food taboo during pregnancy in the study area. The finding suggested that there is a need for strengthening the nutrition counseling components of antenatal care follow-up and planning comprehensive nutrition education through involving important others to dispel such traditional beliefs and prevent food taboo practices in the study community.
A comparison of the bridge-type ROI-C interbody fusion cage system and ACDF with plating and cage system in 2-level anterior cervical discectomy and fusion: A retrospective study
Authors: He Shuangjun , Zhou Zhangzhe , Shao Xiaofeng , Zhang Changhao , Zhou Xinfeng , Wu Shuhua , Chen Kangwu , Wang Yaowei , Qian Zhonglai 
Published: January 2021

Abstract
Objective: A study was conducted to explore the clinical efficacy and radioactive results of the bridge-type ROI-C interbody fusion cage (ROI-C) and anterior cervical discectomy and fusion with plating and cage system (ACDF) for cervical spondylopathy.Methods: From January 2014 to January 2018, 45 patients who underwent operations were retrospectively analyzed, including 24 cases of ROI-C (group A) and 21 cases of ACDF (group B). The operation time, blood loss, Neck Disability Index (NDI), Japanese Orthopaedic Association score (JOA), postoperative complications, imaging results including cervical Cobb angle, and fusion were compared between groups.Results: All patients were successfully treated with surgery. No cerebrospinal fluid leakage, esophageal fistula, or hoarseness occurred after the surgery. The operation time and blood loss in group A were lower than those in group B (P<0.05). During the follow-up period, JOA score increased and NDI score decreased after the operation (P<0.05), but no significant difference was found between the groups (P>0.05). In group A, the incidence of postoperative dysphagia was 12.5%, which returned to normal after 1 month. In group B, the incidence of postoperative dysphagia was 38.1%, which was 19% after 1 month and 4.8% at the final follow-up. In group A, the fusion rate was 83.3% at 3 months after surgery and 100% at the last follow-up. The rate of adjacent level ossification development was 12.5%. In group B, the fusion rate was 85.7% at 3 months after surgery and 100% at the last follow-up. The rate of adjacent level ossification development was 23.8%.Conclusion: Both ROI-C and ACDF achieved satisfactory results, but ROI-C had a shorter operation time, less bleeding, and lower incidence of dysphagia in the short term.
Medical Educators’ Beliefs about the Alignment of Learning Goals, Teaching, and Assessment in the Context of Curriculum Changes: A Qualitative Study &nbsp;Conducted at &nbsp;an Iranian Medical School
Authors: Karami Morteza , Hashemi Nooriyeh , Merrienboer Jeroen van 
Published: January 2021

Abstract
Background: Achieving changing needs, advancing knowledge, and innovations in higher education require the constant changes of medical school curricula and successfully applying the new reforms requires some modifications in the medical educators' core beliefs. The purpose of this study was to describe the medical educators’ beliefs about the alignment of the learning goals, teaching and assessment in the context of the curriculum changes.Method: A qualitative method was used to study the medical educators' beliefs by selecting the faculty participants via a purposeful sampling strategy. This study was conducted at a Medical School in Iran. For the individual interviews, we invited both the professors of the basic sciences and the clinical professors who had worked with the medical students for at least five years. Ten educators were interviewed.Result: The results of the research show that the professors believe that the development of competencies has been abandoned and this is due to the priority of treatment to education in the clinical course and the limited learning experiences. Moreover, the gap between the content and the context and the practicing in the hospital instead of the clinics has reduced the authenticity of the learning environment this situation, and has affected its quality. The non-systematic assessment has also helped to establish.Conclusion: The compartmentalization of the curriculum and the structure of the medical education have caused the beliefs of the professors to be in line with the past perspectives, despite the change in the curriculum, and a change in the structure of the curriculum is necessary to change them.
Analysis of the Gap in PCR Monitoring Availability for Patients with Chronic Myeloid Leukemia in 60 Low- and Middle-Income Countries
Authors: Rowley Seth , Garcia-Gonzalez Patricia , Radich Jerald P , Usherenko Irina , Babigumira Joseph B 
Published: January 2021

Abstract
Purpose: To estimate the resource gap in the polymerase chain reaction (PCR) monitoring for patients with chronic myeloid leukemia (CML) in low- and middle-income countries (LMICs). Methods: We developed a model of demand and supply of PCR monitoring of CML patients in 60 LMICs. PCR testing was assumed to use Cepheid’s GeneXpert® IV system. We included costs of GeneXpert® instruments, uninterrupted power supplies, warranties, calibration kits, test cartridges, and shipping. We calculated the country-specific monetary gap in PCR monitoring, stratified by country priority defined as the availability of tyrosine kinase inhibitors (TKIs) through The Max Foundation initiatives. Results: The five-year gap in PCR monitoring was $29.1 million across all countries, 22% ($6.4 million) in countries with all five TKIs available, 20% ($5.7 million) in countries with four TKIs available, 50% ($14.5 million) in countries with three TKIs available, 8% ($2.2 million) in countries with two TKIs available, and 1% ($0.3 million) in countries with one TKI available. The gap was highest in South Asia (52%; $15.1 million) and lowest in Latin America (6%; $1.9 million). Excluding labor costs, the bulk of the resource needs (86%; $25.2 million) were for procurement of BCR-ABL cartridges. Conclusion: Removing the five-year gap in PCR monitoring capacity for CML in LMICs will require the mobilization of significant resources and will likely lead to better treatment outcomes and reduced treatment costs through optimization of treatment, discontinuation of therapy in appropriate patients, and facilitation of clinical research. Development of streamlined monitoring guidelines for resource-limited countries should be considered.
MiR-144-3p-mediated dysregulation of EIF4G2 contributes to the development of hepatocellular carcinoma through the ERK pathway
Authors: Li Shuangshuang , Shao Jiajia , Lou Guohua , Wu Chao , Liu Yanning , ZHENG MIN 
Published: January 2021

Abstract
Background: Hepatocellular carcinoma (HCC) is one of the most common cancers with high incidence and mortality. However, the underlying mechanisms of HCC still remain unclear. Eukaryotic translation initiation factors (eIFs) have a substantial effect on tumor development. In this study, we were aimed to investigate the role of eukaryotic translation initiation factor 4 gamma 2 (EIF4G2) in HCC.Methods: Western blot (WB) of 30 paired HCC tissues and tissue microarrays (TMAs) conducted by immunohistochemistry (IHC) in 89 paired HCC samples were performed to assess EIF4G2 expression. Clone formation, real-time cell analysis (RTCA), wound healing and transwell assays were adopted to evaluate the role of EIF4G2 on HCC cell proliferation, migration and invasion abilities. The function of EIF4G2 in HCC tumor growth was assessed in a xenograft nude mouse model in vivo. The regulation of EIF4G2 by miR-144-3p was performed by luciferase reporter assay and WB.Results: The EIF4G2 protein was clearly upregulated in HCC tissues, and high EIF4G2 expression was closely related to HCC prognosis. EIF4G2 silencing could inhibit HCC cell growth and metastasis in vitro, and suppress tumorigenesis in vivo by repressing the ERK signaling pathway. The results of luciferase reporter assays, WB and IHC staining verified that EIF4G2 was negatively regulated by miR-144. And re-expression of EIF4G2 could partially reverse the inhibiting effect of miR-144 in HCC.Conclusion: In summary, our study revealed the role of EIF4G2 in HCC development via the activation of the ERK pathway. We also found that EIF4G2 could be negatively regulated by the tumor suppressor miR-144. Our investigations indicated that EIF4G2 might be a promising therapeutic target in HCC.
Motivation, satisfaction and perceived benefits of volunteering with the Cardiovascular Health Awareness Program (CHAP): A survey of French-speaking volunteers in Quebec
Authors: Kaczorowski Janusz , Moussa Hanaa , Lussier Marie-Thérèse , Girard Magali 
Published: January 2021

Abstract
Background: Locally recruited and trained volunteers are a vital component of the Cardiovascular Health Awareness Program (CHAP), a community-based cardiovascular disease prevention program that has been implemented across different settings and targeting different populations. As part of expanding and adapting CHAP to new settings, we conducted a pragmatic cluster randomized controlled trial to implement CHAP targeting French-speaking elderly residents of subsidized social housing buildings in Québec and Ontario. As there is some evidence that the culture of volunteering might be different for English-speakers than their French-speaking counterparts, our objective was to generate a profile of French-speaking CHAP volunteers and to compare it with English-speaking volunteers in Ontario who participated in an earlier community-based CHAP program and with volunteers in Canada as reported in the Statistics Canada's General Social Survey on Giving, Volunteering and Participating (CSGVP). Methods: A cross-sectional survey was administered online or by telephone to French-speaking volunteers who participated in at least one CHAP session held in subsidized social housing in Montérégie (Québec, Canada) between March 2018 and June 2019. Results: The response rate was 79% (30/38), majority were female (87%, 26/30), retired or semi-retired (83%; 25/30), almost two-thirds (63%, 19/30) had previous work experience in the healthcare field, and 40% (12/30) had more than 10 years of volunteering experience. These characteristics were similar both to those of general profile of volunteers in Canada and to English-speaking volunteers in Ontario who participated in an earlier pharmacy-based CHAP program. The top 5 reasons for volunteering among French-speaking CHAP volunteers were identical to those reported in CSGVP: making a contribution to the community; using one’s skills and experience; improving one’s sense of well-being or health; exploring one’s own strengths; and networking with or meeting people. The majority of French-speaking CHAP volunteers, like their English counterparts in Ontario, reported a high level of satisfaction with their participation in the program, enjoyed their interaction with participants, and gained new knowledge. Conclusions: French-speaking CHAP volunteers in Quebec share many similarities with English-speaking CHAP volunteers in Ontario and volunteers in Canada in terms of background, motivation, satisfaction and perceived benefits of volunteering.
Ennius o ‘manum conserere’ według ‘Noctes Atticae’ Aulusa Gelliusa
Authors: Zabłocki Jan 
Published: January 2021

Pewne wyrażenia prawne, znane niegdyś ogólnie, z czasem stają się niezrozumiałe. Ciekawym przykładem tego jest sformułowanie ex iure manum consertum omawiane przez antykwarystę. Aulus Gellius zapytał Sulpiciusa Apollinarisa słynnego wówczas znawcę literatury co oznaczają słowa ex iure manum consertum. Ten w odrzekł mu, że jest znawcą literatury a nie prawa, a tego sformułowania nie ma na kartach Annales. Kiedy jednak Gellius wyrecytował tę frazę z dzieła Enniusa, Supicius Apollinaris skonstatował, że Ennnius zaczerpnął je od jakiegoś jurysty i odesłał Gelliusa do jurystów i ich dzieł. Gellius po zbadaniu sprawy doszedł do wniosku, że, jeśli toczył się spór (in iure) o jakąś rzecz (de re) znajdującą się (in re praesenti) przed pretorem, czy to o grunt, czy o coś innego, trzymając tę rzecz w ręku (in iure manum consererent), dokonywano jej windykacji w uroczystych słowach. Z czasem, po rozszerzeniu granic państwa, pretorzy nadmiernie zajęci w sprawach dotyczących windykacji udawali się niechętnie daleko położonych spornych rzeczy. Zatem, za milczącym przyzwoleniem (tacito consensu), wbrew Ustawie XII Tablic zezwolili, aby toczący spór udawali się na grunt, o który toczył się spór, i przynosili grudkę jakąś z niego, jakby całą ziemię przed pretora na sąd (in ius) i przy pomocy tej grudki (ex iure manum consertum), tak jakby na całym gruncie dokonywali windykacji.