bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification of an Altered Matrix Signature in Kidney Aging and Disease
Authors: Randles Michael J. , Lausecker Franziska , Kong Qingyang , Suleiman Hani , Reid Graeme , Kolatsi-Joannou Maria , Davenport Bernard , Tian Pinyuan , Falcone Sara , Potter Paul , Van Agtmael Tom , Norman Jill T. , Long David A. , Humphries Martin J. , Miner Jeffrey H. , Lennon Rachel 
Published: May 2021

Significance Statement
Abnormal extracellular matrix is a histologic feature of kidney aging and disease. However, a comprehensive molecular basis for altered matrix is not well understood. Ultrastructural and proteomic studies in mouse models of genetic kidney disease and human tissue define a molecular basis for altered matrix, which has common features across aging and disease progression. Broadly, basement membrane components are reduced, interstitial matrix proteins are increased, and this is coupled with altered cell adhesion and metabolic processes. Furthermore, a signature of altered matrix proteins appears before ultrastructural defects and could have utility as biomarkers of kidney health. Mechanistically, this altered kidney matrix may initiate abnormal kidney cell–matrix and immune cell–matrix interactions, which therapy could target.

Background
Accumulation of extracellular matrix in organs and tissues is a feature of both aging and disease. In the kidney, glomerulosclerosis and tubulointerstitial fibrosis accompany the decline in function, which current therapies cannot address, leading to organ failure. Although histologic and ultrastructural patterns of excess matrix form the basis of human disease classifications, a comprehensive molecular resolution of abnormal matrix is lacking.

Methods
Using mass spectrometry–based proteomics, we resolved matrix composition over age in mouse models of kidney disease. We compared the changes in mice with a global characterization of human kidneymatrix during aging and to existing kidney disease datasets to identify common molecular features.

Results
Ultrastructural changes in basement membranes are associated with altered cell adhesion and metabolic processes and with distinct matrix proteomes during aging and kidney disease progression in mice. Within the altered matrix, basement membrane components (laminins, type IV collagen, type XVIII collagen) were reduced and interstitial matrix proteins (collagens I, III, VI, and XV; fibrinogens; and nephronectin) were increased, a pattern also seen in human kidney aging. Indeed, this signature of matrix proteins was consistently modulated across all age and disease comparisons, and the increase in interstitial matrix was also observed in human kidney disease datasets.

Conclusions
This study provides deep molecular resolution of matrix accumulation in kidney aging and disease, and identifies a common signature of proteins that provides insight into mechanisms of response to kidney injury and repair.
A Biallelic Frameshift Mutation in Nephronectin Causes Bilateral Renal Agenesis in Humans
Authors: Dai Lei , Li Jingzhi , Xie Liangqun , Wang Weinan , Lu Yang , Xie Mingkun , Huang Jingrui , Shen Kuifang , Yang Hui , Pei Chenlin , Zhao Yanhua , Zhang Weishe 
Published: May 2021

Significance Statement
Bilateral renal agenesis (BRA) is a lethal form of congenital anomaly of the kidney and urinary tract, usually resulting in perinatal death or justifying termination of pregnancy. The exact causes of this disease are not clear, although genetic factors are considered important. In this study, we describe a pathological homozygous frameshift variant (NPNT:NM_001184690:exon8:c.777dup/p.Lys260*) that is responsible for a recessive form of BRA within a family. A knock-in mouse model mimicking the mutation we found in the family was established and replicated the phenotype of BRA. These results reveal a new genetic cause of BRA and identify a new target for genetic diagnosis, prenatal diagnosis, and preimplantation diagnosis for families with BRA.

Background
Bilateral renal agenesis (BRA) is a lethal con genital anomaly caused by the failure of normal development of both kidneys early in embryonic development. Oligohydramnios on fetal ultrasonography reveals BRA. Although the exact causes are not clear, BRA is associated with mutations in many renal development genes. However, molecular diagnostics do not pick up many clinical patients. Nephronectin (NPNT) may be a candidate protein for widening diagnosis. It is essential in kidney development, and knockout of Npnt in mice frequently leads to kidney agenesis or hypoplasia.

Methods
A consanguineous Han family experienced three cases of induced abortion in the second trimester of pregnancy, due to suspected BRA. Whole-exome sequencing (WES)–based homozygosity mapping detected underlying genetic factors, and a knock-in mouse model confirmed the renal agenesis phenotype.

Results
WES and evaluation of homozygous regions in II:3 and II:4 revealed a pathologic homozygous frameshift variant in NPNT (NM_001184690:exon8:c.777dup/p.Lys260*), which leads to a premature stop in the next codon. The truncated NPNT protein exhibited decreased expression, as confirmed in vivo by the overexpression of WT and mutated NPNT. A knock-in mouse model homozygous for the detected Npnt mutation replicated the BRA phenotype.

Conclusions
A biallelic loss-of-function NPNT mutation causing an autosomal recessive form of BRA in humans was confirmed by the corresponding phenotype of knock-in mice. Our results identify a novel genetic cause of BRA, revealing a new target for genetic diagnosis, prenatal diagnosis, and preimplantation diagnosis for families with BRA.
Abdominal cocoon
Authors: Manohar Aishwarya Emerald , Sabarigirieasen M. , Nedunchezhian S. , Kannan R. 
Published: May 2021

Cocoon abdomen is relatively a rare entity that is encountered less often during our surgical practice and is usually seen in young females. We present two cases of abdominal cocoon in middle age and elderly males both having different clinical presentations and management. Firstly, we had a 34 years male was admitted with complaints of diffuse abdominal pain and obstipation for 3 days. Abdomen was grossly distended with sluggish bowel sounds and computed tomography (CT) was suggestive of dilated small bowel loops. Laparotomy revealed a thin peritoneal covering encapsulating the small bowel loops and a band arising from the right parietal wall to the ileum. Band release and adhesiolysis was performed. He had an uneventful post-operative recovery and is on follow up now. Our second patient was a 60 years male, who presented with diffuse abdominal pain and distension for 2 weeks and vomiting for 4 days. There was abdominal distension, visible intestinal persistalsis, fullness of the flanks, exaggerated bowel sounds and a irreducible right inguinal hernia. CT revealed obstructed right inguinal hernia with large bowel as content. The right inguinal region was explored and a thick shiny membrane was found covering the contents of the sac. After adhesiolysis and herniorraphy, patient initially recovered well, but later succumbed due to medical causes unrelated to surgery. It is clear that both these cases represent two distinct clinical spectrum of a similar pathological process which has to be considered as one of the differential diagnoses in patients with equivocal clinical findings.
A pós-graduação stricto sensu do Brasil: espaço de formação de professores universitários
Authors: Junges Junior Mário Luiz , Stefanello Flávia , Vieira Josimar De Aparecido 
Published: May 2021

Este artigo foi desenvolvido com o objetivo de avaliar as possibilidades de formação de professores universitários nos programas de pós-graduação stricto sensu das instituições públicas de ensino superior no Brasil. Para tanto, foi elaborada uma pesquisa de abordagem qualitativa que, quanto aos objetivos, define-se como exploratória e descritiva, tendo como procedimentos a pesquisa bibliográfica e documental. O processo de análise dos dados foi realizado de forma dinâmica, onde buscou-se teorizar sobre os dados, produzindo o confronto entre a abordagem teórica anterior e o que a investigação aportou de singular como contribuição. Encontra-se estruturado de modo que apresenta o percurso metodológico, considerações sobre o processo de formação do professor universitário, o andamento dos programas de pós-graduação stricto sensu, os parâmetros e contradições presentes neste processo e as considerações finais. Esses pontos indicam que a formação de professores do ensino superior nos programas de pós-graduação analisados tem se distanciado dos principais aspectos que envolvem a formação de professores.
Fatty acid profile in brain and hepatic tissues from pigs supplemented with canola oil
Authors: Pereira Martins da Silva Julia , Assis Costa Karine , Vezzoni Almeida Vivian , Lehmann Coutinho Luiz , Pereira Martins da Silva Bruna , Silva Mello Cesar Aline 
Published: May 2021

Canola oil is an important source of oleic acid, in addition to being an accessible source for its use in the production of pigs’ diets. Oleic acid in turn is a type of unsaturated fatty acid that in pork is beneficial for human health. Therefore, this study was conducted to describe the fatty acid profile in brain and liver tissues from pigs supplemented with canola oil for 98 days, during the growth and finishing phases. For the analysis was used eighteen male pigs that had free access to feed and water throughout the experimental period. Dietary treatment consisted of corn-soybean meal growing-finishing diets supplemented with 3% fat from canola oil (CO). To obtain the fatty acid profile, the lipids from each tissue were cold extracted using the adapted method from Bligh and Dyer, methylated, and posteriorly injected in a gas chromatograph to obtain the fatty acid profile of the tissue. In both tissues there was a greater abundance of saturated fatty acids (stearic acid). The most abundant monounsaturated fatty acid was the oleic acid. Regarding polyunsaturated fatty acids, in the liver the most abundant was linoleic acid and in the brain docosahexaenoic acid. In summary, animals' diet influences the fatty acid profile in different tissues. Such modifications can increase unsaturated fatty acids concentration in relation to saturated, making pork healthier for human consumption.
Matérias estranhas em polpa de acerola
Authors: GOMES FERREIRA ANAMARIA , Urcina da Cruz Maria Lúcia , Santos Rocha da Silva Larissa , Castro Porto Bruna 
Published: May 2021

Com a busca por produtos mais saudáveis e práticos durante o preparo, o consumo e a comercialização de polpas de fruta vêm aumentando. Polpa de fruta consiste em um produto que não é submetido a processos de diluição, fermentação, concentração e que apresenta o teor mínimo de sólidos totais oriundos da parte comestível da fruta por técnica adequada. As matérias estranhas previstas na legislação brasileira e encontradas em alimentos são qualquer material não constituinte do produto associado a condições ou práticas inadequadas de produção, manipulação, armazenamento e/ou distribuição. O objetivo do presente estudo foi investigar a presença de matérias estranhas em polpas de acerola comercializadas na cidade de Salinas - MG. Para isso, as amostras foram diluídas e filtradas em papel de filtro, o qual foi submetido a observação em microscópio óptico. Nas amostras analisadas, foram encontrados fragmentos de inseto, tecido, e outras matérias estranhas não identificadas, evidenciando condições higiênico-sanitárias irregulares no processo de fabricação classificando-as como impróprias para o consumo.
Resenha do Livro “Audiovisualidades: elaborar com Foucault”
Authors: Carvalho Vívian De Nazareth Santos 
Published: May 2021

MILANEZ, Nilton. (2019). Audiovisualidades: elaborar com Foucault. Londrina: Eduel; Guarapuava: Ed. Unicentro. Quais são as regras de formação para os discursos das audiovisualidades? Que objetos se levantam em direção à constituição de um filme? Estas são algumas questões que o livro “Audiovisualidades: Elaborar com Foucault” traz ao leitor. A obra, lançada em 2019, e de autoria de Nilton Milanez, parte dos postulados teórico-metodológicos presentes em “A Arqueologia do Saber” (2008), de Michel Foucault, para pensar as audiovisualidades e, por meio delas, compreender aquilo que somos, como nos organizamos e o que nos torna sujeitos no presente.
“Depois a louca sou eu”: estratégias biopolíticas e produção de subjetividade em discursos sobre relacionamento abusivo
Authors: Vieira da Silva Francisco 
Published: May 2021

O artigo discute as condições de possibilidade que fazem irromper discursos acerca do relacionamento abusivo na contemporaneidade, através do exame do funcionamento de estratégias biopolítica na produção de tais discursos e de como o sujeito que enuncia acerca do relacionamento abusivo se constitui numa relação consigo mesmo e com o outro. O referencial teórico reside nas investigações de Foucault acerca do discurso, enunciado, sujeito, poder, saber e biopolítica. Trata-se de um estudo de cunho descritivo-interpretativo, prioritariamente qualitativo. As condições de possibilidade responsáveis pela emergência de discursos sobre relacionamento abusivo relacionam-se às inflexões do movimento feminista na articulação com a cultura terapêutica materializada nas mídias digitais. Assim, as mulheres, ao enunciarem sobre as relações abusivas, efetuam um trabalho sobre si e sobre o outro, de modo a fazer funcionar as estratégias bipolíticas em torno da saúde e bem-estar da mulher no âmbito dos relacionamentos amorosos.
Medication adherence and persistence of psoriatic arthritis patients treated with biological therapy in a specialty pharmacy in Brazil: a prospective observational study
Authors: Souza Ana F. , Da Silva Michael R. , Dos Santos Jéssica B. , Almeida Alessandra M. , Acurcio Francisco A. , Alvares-Teodoro Juliana 
Published: May 2021

Background: Pharmaceutical services in Brazil provide access, supply, and rational use of drugs for all population and an effort has been made to improve the quality of these services. Biological drugs are high-cost drugs supplied in Brazil that can inhibit disease progression and improve the quality of life of psoriatic arthritis (PsA) patients. However, some patients did not achieve therapeutic goals.
Objective: To evaluate the medication adherence and persistence of PsA patients treated with tumor necrosis factor inhibitors (anti-TNF) drugs and their associated factors.
Methods: A prospective observational study was performed at a single-specialty pharmacy in Belo Horizonte, Brazil. Medication adherence, persistence, and clinical outcomes were evaluated at 12 months of follow-up. Medication persistence was historically compared to overall PsA patients treated in Brazil. Associated factors were identified through log-binomial regression.
Results: One hundred ninety-seven PsA patients were included in the study, of whom 147 (74.6%) and 142 (72.1%) had medication adherence and persistence, respectively. Patients treated with infliximab presented the highest adherence (90.5%) and persistence rate (95.2%) in comparison to patients treated with other drugs, except for adalimumab versus infliximab for adherence outcome. All clinical measures significantly improved in patients with medication adherence and persistence. Medication persistence was higher for patients attended by specialty pharmacy than other PsA patients in Brazil. The associated factors to higher medication adherence were lower disease activity by BASDAI, being non-white race, and intravenous drug use. The associated factors to higher medication persistence were lower disease activity by Bath Ankylosing Spondylitis Activity Index (BASDAI), intravenous drug use, non-use of corticoids and non-steroidal anti-inflammatory drugs, and comorbidity.
Conclusions: Patients with medication adherence and persistence had significant improvements in clinical measures, functionality, and quality of life. High medication adherence and persistence to biological therapy were observed and associated with lesser disease activity at baseline. Also, medication persistence to PsA patients attended in specialty pharmacy was higher than the overall PsA population in Brazil, which indicates the importance of pharmaceutical services to provide health care and promote the effectiveness and safety of biological therapies.
Using TDFragMapper: a high-performance tool to assess experimental parameters in top-down proteomics
Authors: Lima Diogo Borges , Dhenin Jonathan , Dupré Mathieu , Chamot-Rooke Julia 
Published: May 2021

Abstract
Here we present a new software-tool for visualizing fragment ions and sequence coverage of intact proteins in top-down mass spectrometry. TDFragMapper combines the data arising from multiple and diverse tandem mass spectrometry experiments of intact proteins. Our tool maps fragment ions onto the protein backbone sequence and allows for a rapid comparison of the results obtained when varying experimental parameters in tandem mass spectrometry (MS/MS) experiments of intact proteins: fragmentation method, selected precursor charge state, activation level, technical replicate. In summary, TDFragMapper is a unique software-tool that allows an easy selection of the best experimental conditions leading to the highest confidence in intact protein sequence identification.
Pregnancy outcomes among women with thyrotoxicosis a retrospective cohort study
Authors: Harn-a-morn Panwad , Dejkhamron Prapai , Tongsong Theera , Luewan Suchaya 
Published: May 2021

Abstract
Objective: To compare adverse outcomes between: 1) pregnant women with thyrotoxicosis and low risk pregnancies, 2) pregnant women with thyrotoxicosis requiring no anti-thyroid drug (ATD) and low risk pregnancies, and 3) those treated with methimazole (MMI) and propylthiouracil (PTU)Methods: The medical records of singleton pregnancies with thyrotoxicosis were comprehensively reviewed. Low-risk pregnancies matched for age and parity were randomly recruited as controls. The obstetric outcomes were compared between both groups, and the outcomes of various subgroups of the study group were also compared.Results: A total of 408 pregnant women with thyrotoxicosis were recruited. Compared with the controls, the women of the study group had significantly higher rates of low birth weight (LBW) (23.7% vs 17.7%; p:0.036), preterm birth (19.3% vs 12.3%; p:0.007), preeclampsia (8.5% vs 4.4%; p: 0.019) and cesarean section (21.5% vs 16.0%; p:0.046). In the study group (thyrotoxicosis), 67, 127, and 158 patients were treated with MMI, PTU and no anti-thyroid drug (ATD), respectively. All obstetric outcomes were comparable between the women treated with PTU and those with MMI, and between the controlled and uncontrolled groups. However, women who needed ATD had significantly higher rates of LBW and preterm birth than those without medications.Conclusions: Thyrotoxicosis, whether treated or not needing ATDs, was significantly associated with an increased risk of adverse pregnancy outcomes. Also, active disease, indicated by the need for ATD significantly increased the risk of such adverse outcomes, whereas the patients treated with MMI or PTU had comparable adverse outcomes.
SalmoSim: The Development of a Three-Compartment In Vitro Simulator of the Atlantic Salmon GI tract and Associated Microbial Communities
Authors: Kazlauskaite Raminta , Cheaib Bachar , Heys Chloe , Ijaz Umer , Connelly Stephanie , Sloan William , Russell Julie , Rubio Laura , Sweetman John , Kitts Alex , McGinnity Philip , Lyons Philip , Llewellyn Martin 
Published: May 2021

Abstract
BackgroundAtlantic salmon are a fish species of major economic importance. Innovative strategies are being sought to improve salmon feeds and feed additives to enhance fish performance, welfare, and the environmental sustainability of the aquaculture industry. There is still a lack of knowledge surrounding the importance and functionality of the salmon gut microbiome in fish nutrition. In vitro gut model systems might prove a valuable tool to study the effect of feed, and additives, on the host’s microbial communities. Several in vitro gut models targeted at monogastric vertebrates are now in operation. Here, we report the development of an Atlantic salmon gut model, SalmoSim, to simulate three gut compartments (stomach, pyloric caecum and midgut) and associated microbial communities. ResultsThe gut model was established in a series of linked bioreactors seeded with biological material derived from farmed adult marine phase salmon. We first aimed to achieve a stable microbiome composition representative of founding microbial communities derived from Atlantic salmon. Then, in biological triplicate, the response of the in vitro system to two distinct dietary formulations (fish meal and fish meal free) was compared to a parallel in vivo trial over forty days. Metabarcoding based 16S rDNA sequencing, qPCR, ammoniacal nitrogen and volatile fatty acid measurements were undertaken to survey the microbial community dynamics and function. SalmoSim microbiomes were indistinguishable (p=0.230) from their founding inocula at 20 days and the most abundant genera (e.g. Psycrobacter, Staphylococcus, Pseudomonas) proliferated within SalmoSim (OTUs accounting for 98% of all reads shared with founding communities). Real salmon and SalmoSim responded similarly to the introduction of novel feed, with majority of the taxa (96% Salmon, 97% SalmoSim) unaffected, while a subset of taxa (e.g. a small fraction of Psychrobacter) were differentially affected across both systems. Consistent with a low impact of the novel feed on microbial fermentative activity, volatile fatty acids profiles were not significantly different in SalmoSim pre- and post-feed switch.ConclusionBy establishing stable and representative salmon gut communities, this study represents an important step in the development of an in vitro gut system as a tool for the improvement of salmon nutrition and welfare. This system aims to be utilised as a pre-screening tool for new feed ingredients and additives, as well as being used to study antimicrobial resistance and transfer, and fundamental ecological processes that underpin microbiome dynamics and assembly.