bluuebunny/crossref_metadata_2025_split huggingface dataset:
MiR-1-3p Suppresses Colorectal Cancer Cell Proliferation and Metastasis by Inhibiting YWHAZ-Mediated Epithelial–Mesenchymal Transition
Authors: Du Guanghong , Yu Xuelian , Chen Yun , Cai Wangting 
Published: February 2021

BackgroundColorectal cancer (CRC) is regarded as one of the most common malignancies in the world. MiR-1-3p was reported to be a tumor suppressor in CRC. However, the mechanisms have not been fully elucidated.MethodsTo identify CRC-associated miRNA, microarray data set GSE30454 was downloaded from the Gene Expression Omnibus database (GEO), and miR-1-3p was screened out as a candidate. The expression of miR-1-3p was detected using quantitative real-time polymerase chain reaction (qRT-PCR) in CRC cell lines and tissues. CCK-8 assay and transwell invasion assay were performed to determine CRC cell line proliferation and invasion, respectively. The levels of YWHAZ and EMT-associated proteins were detected using western blotting.ResultsBioinformatic analysis showed that miR-1-3p was downregulated in CRC tissues, which is verified by our experimental validation. The overexpression of miR-1-3p significantly suppressed CRC cell proliferation and invasion. Further studies showed that YWHAZ was a direct target of miR-1-3p and mediated epithelial–mesenchymal transition (EMT) modulated by miR-1-3p.ConclusionOur results demonstrated that miR-1-3p suppresses colorectal cancer cell proliferation and metastasis through regulating YWHAZ-mediated EMT, which may support a novel therapeutic strategy for CRC patients.
In silico Analyses of Immune System Protein Interactome Network, Single-Cell RNA Sequencing of Human Tissues, and Artificial Neural Networks Reveal Potential Therapeutic Targets for Drug Repurposing Against COVID-19
Authors: López-Cortés Andrés , Guevara-Ramírez Patricia , Kyriakidis Nikolaos C. , Barba-Ostria Carlos , León Cáceres Ángela , Guerrero Santiago , Ortiz-Prado Esteban , Munteanu Cristian R. , Tejera Eduardo , Cevallos-Robalino Doménica , Gómez-Jaramillo Ana María , Simbaña-Rivera Katherine , Granizo-Martínez Adriana , Pérez-M Gabriela , Moreno Silvana , García-Cárdenas Jennyfer M. , Zambrano Ana Karina , Pérez-Castillo Yunierkis , Cabrera-Andrade Alejandro , Puig San Andrés Lourdes , Proaño-Castro Carolina , Bautista Jhommara , Quevedo Andreina , Varela Nelson , Quiñones Luis Abel , Paz-y-Miño César 
Published: February 2021

Background:There is pressing urgency to identify therapeutic targets and drugs that allow treating COVID-19 patients effectively.Methods:We performedin silicoanalyses of immune system protein interactome network, single-cell RNA sequencing of human tissues, and artificial neural networks to reveal potential therapeutic targets for drug repurposing against COVID-19.Results:We screened 1,584 high-confidence immune system proteins in ACE2 and TMPRSS2 co-expressing cells, finding 25 potential therapeutic targets significantly overexpressed in nasal goblet secretory cells, lung type II pneumocytes, and ileal absorptive enterocytes of patients with several immunopathologies. Then, we performed fully connected deep neural networks to find the best multitask classification model to predict the activity of 10,672 drugs, obtaining several approved drugs, compounds under investigation, and experimental compounds with the highest area under the receiver operating characteristics.Conclusion:After being effectively analyzed in clinical trials, these drugs can be considered for treatment of severe COVID-19 patients. Scripts can be downloaded athttps://github.com/muntisa/immuno-drug-repurposing-COVID-19.
Bulleyaconitine A Inhibits Morphine-Induced Withdrawal Symptoms, Conditioned Place Preference, and Locomotor Sensitization Via Microglial Dynorphin A Expression
Authors: Zhao Meng-Jing , Wang Mi-Ya , Ma Le , Ahmad Khalil Ali , Wang Yong-Xiang 
Published: February 2021

Bulleyaconitine A (BAA), a C19-diterpenoid alkaloid, has been prescribed as a nonnarcotic analgesic to treat chronic pain over four decades in China. The present study investigated its inhibition in morphine-induced withdrawal symptoms, conditioned place preference (CPP) and locomotor sensitization, and then explored the underlying mechanisms of actions. Multiple daily injections of morphine but not BAA up to 300 μg/kg/day into mice evoked naloxone-induced withdrawal symptoms (i.e., shakes, jumps, genital licks, fecal excretion and body weight loss), CPP expression, and locomotor sensitization. Single subcutaneous BAA injection (30–300 μg/kg) dose-dependently and completely attenuated morphine-induced withdrawal symptoms, with ED50 values of 74.4 and 105.8 μg/kg in shakes and body weight loss, respectively. Subcutaneous BAA (300 μg/kg) also totally alleviated morphine-induced CPP acquisition and expression and locomotor sensitization. Furthermore, subcutaneous BAA injection also specifically stimulated dynorphin A expression in microglia but not astrocytes or neurons in nucleus accumbens (NAc) and hippocampal, measured for gene and protein expression and double immunofluorescence staining. In addition, subcutaneous BAA-inhibited morphine-induced withdrawal symptoms and CPP expression were totally blocked by the microglial metabolic inhibitor minocycline, dynorphin A antiserum, or specific KOR antagonist GNTI, given intracerebroventricularly. These results, for the first time, illustrate that BAA attenuates morphine-induced withdrawal symptoms, CPP expression, and locomotor sensitization by stimulation of microglial dynorphin A expression in the brain, suggesting that BAA may be a potential candidate for treatment of opioids-induced physical dependence and addiction.
High Expression of Tumor Abnormal Protein Preoperatively Predicts Poor Prognosis of Patients With Esophageal Squamous Cell Carcinoma
Authors: Cheng Yuanjun , Fang Qianru , Chen Yongbing , Zang Guohui , Yao Jie 
Published: February 2021

Background: Esophageal squamous cell carcinoma (ESCC) acts as a fatal malignant tumor among human beings and is marked by late-stage diagnosis, frequent recurrence, metastasis, and therapy resistance. Tumor abnormal protein (TAP) remarkably affects cancer development and progression of human cancers. TAP has been shown to be a biomarker for gastric and lung cancer progression. Nevertheless, the clinical value exhibited by TAP for ESCC has not been well-explained in the current literature.Methods: The present study included 183 ESCC cases who received surgical resection and 183 cases who had normal physical checkup from March 2013 to January 2015 at the People's Hospital of Chizhou, and used the TAP detection agent for evaluating the TAP relative level.Results: As found, ESCC patients presented an obviously higher TAP expression relative to cases who had normal physical checkup. Moreover, TAP expression was significantly downregulated after surgery. Furthermore, the TAP expression was correlated with gender, smoking, pathologic differentiation, and pN stage, but not with age, tumor location, surgical type, pT stage, and vascular invasion. High expression of TAP was significantly correlated with poorer overall survival (OS) rate in ESCC patients. TAP was an independent prognostic predictor in ESCC patients, based on the multivariate survival analysis.Conclusion: The study reveals how TAP upregulation promotes ESCC malignant progression, and concludes that TAP acts as the therapeutic target and potential biomarker specific to ESCC.
Kartu Bergambar Meningkatkan Pengetahuan, Sikap, dan Prosedur Mencuci Tangan Anak Kelas 5 Sekolah Dasar Di SD Muhammadiyah 14 Balayudha Palembang Tahun 2019
Authors: Hasanah Tri Meilana , Majid Yudi Abdul , Tirtayanti Sri 
Published: February 2021

Rendahnya pengetahuan anak usia sekolah tentang pentingnya cuci tangan berkaitan dengan paparan informasi dan peran serta tenaga kesehatan dalam memberikan pendidikan kesehatan disekolah. Untuk mengatasi masalah tersebut diperlukan peran petugas kesehatan atau perawat. Salah satu peran perawat dalam hal ini sebagai edukator. Pendidikan kesehatan pada anak usia sekolah dasar harus disesuaikan dengan perkembangan pada dirinya. Salah satu media yang tepat untuk pendidikan kesehatan pada anak sekolah dasar yaitu media kartu bergambar yang sudah dimodifikasi dengan gambar, tulisan tentang prosedur mencuci tangan dan disertai dengan warna yang menarik. Tujuan Penelitian ini yaitu mengetahui Perbedaan Pengetahuan, Sikap, Dan Perilaku Prosedur Mencuci Tangan Anak Kelas 5 Sekolah Dasar Di SD Muhammadiyah 14 Balayudha Palembang Tahun 2019. Desain penelitian yang digunakan yaitu pre-eksperimental dengan one group pre test dan post test design dengan uji analisa Wilcoxon Rank Test dan jumlah sampel 175 responden. Berdasarkan analisa univariat diketahui nilai median pengetahuan sebelum dilakukan pendidikan kesehatan 9,00 dan setelah pendidikan kesehatan 11,00. Nilai median sikap sebelum pendidikan kesehatan 41,00 dan setelah pendidikan kesehatan 47,00. Nilai median prosedur sebelum pendidikan kesehatan 2,00 dan setelah pendidikan kesehatan 6,00. Hasil uji statistik wilcoxon rank test didapatkan nilai P Value 0,000. Dapat disimpulkan bahwa ada perbedaan pengetahuan, sikap, dan prosedur cuci tangan sebelum dan sesudah dilakukan pendidikan kesehatan dengan media kartu bergambar pada anak kelas 5 sekolah dasar di SD Muhammadiyah 14 Balayudha Palembang.
METHOD OF GIVING NURSING CONNECTION TO THE IMPLEMENTATION OF DISCHARGE PLANNING
Authors: Imelda Tiara , Yulia Sri , Muliyadi Muliyadi 
Published: February 2021

ABSTRAK
Latar Belakang : Pelaksanaan discharge planning yang belum optimal dilaksanakan, dapat menyebabkan tidak efektifnya kontinuitas perawatan di rumah, meningkatkan resiko keparahan (severity), dan dirawat kembali (readmission). Tujuan : Mengetahui hubungan karakteristik perawat dan metode pemberian asuhan keperawatan dengan pelaksanaan discharge planning di Ruang Rawat Inap RSUD Palembang BARI Tahun 2019. Metode : Desain deskriptif analitik dengan cross-sectional. Jumlah sampel sebanyak 30 perawat dengan tehnik purposive sampling. Penelitian menggunakan instrumen kuesioner. Uji statistik Chi Square dan t-test. Hasil : Ada hubungan antara metode pemberian asuhan keperawatan dengan pelaksanaan discharge planning (p=0,020 < ? = 0,05). Sedangkan usia (p=0,742), jenis kelamin (p=0,672), pendidikan (p=0,165), masa kerja (p=0,618) tidak ada hubungan dengan pelaksanaan discharge planning. Simpulan : Metode pemberian asuhan keperawatan merupakan faktor yang berhubungan dengan pelaksanaan discharge planning. Perlunya penambahan SDM atau perawat di Ruangan pada saat shift malam.
HUBUNGAN BUDAYA ORGANISASI DENGAN KINERJA PERAWAT DALAM MEMBERIKAN PELAYANAN KEPERAWATAN DI RUMAH SAKIT MUHAMADIYAH TAHUN 2019
Authors: Utami Tri , Romiko Romiko , Yulia Sri 
Published: February 2021

Budaya Organisasi merupakan aspek penting dalam terciptanya sistem kinerja perawat yang baik, Budaya organisasi yang baik diruang rawat inap akan mempengaruhi kinerja perawat menjadi lebih baik dan dapat menciptakan pelayanan yang bermutu. Mengetahui hubungan budaya organisasi dengan kinerja perawat dalam memberikan pelayanan keperawatan di Rumah Sakit Muhammadiyah Palembang: Penelitian ini bersifat kuantitatif dengan pendekatan Cross Sectional, teknik pengambilan sampel menggunakan Purposive Sampling pada penelitian ini menggunakan dua yaitu kuesioner budaya organisasi dan kinerja perawat dengan menggunakan uji statistik Chi Square dan analisa univariat memiliki 27 responden (54%) perawat yang mempersepsikan budaya organisasi tinggi dan kinerja perawat yang memiliki berkinerja baik terdapat 28 responden (56%) Ada hubungan signifikan antara budaya organisasi dengan kinerja perawat dalam memberikan pelayanan keperawatan (p value =0,000) Budaya organisasi adalah aspek yang berhubungan pada kinerja perawat sehingga diharapkan rumah sakit harus dapat memperhatikan indikator kinerja perawat dalam menciptakan pelayanan keperawatan yang bermutu.
HUBUNGAN PENGETAHUAN DENGAN PERILAKU SEKSUAL PADA REMAJA DI SMK MJPS 1 KOTA TASIKMALAYA
Authors: Nurdianti Reni , Marlina Lina , Sumarni Sumarni 
Published: February 2021

Nowadays, sex among teenagers is not taboo. Sexual behavior among adolescents tends to increase, especially unmarried adolescents. In the24 countries in North America and Europe show that adolescent sexual behavior begins at the age of 15. The survey was conducted to 33,943 in 24 countries carried out by a French Non-Governmental Organization (NGO), showing that 13.2% of adolescents have been sexually active since the age of 15 and do not use contraceptives. Meanwhile, 82% use contraception.The purpose of this study was whether there was a relationship between knowledge and sexual behavior in adolescents at SMK MJPS 1 Tasikmalaya city.
This research method is descriptive analytic with cross-sectional correlation approach. Samples taken in this study were students of class X-XII teenagers at SMK MJPS 1 Tasikmalaya city by using the sampling technique used was random sampling.
The results of the respondent's data taken were 89 people. From the data obtained, students with sufficient knowledge amounted to 60 people or (67.4%) and a small number of students with less knowledge amounted to 7 people (7.9%) with less behavior amounted to 37 people or (41.6%) and good behavior amounted to 24 people or (27%). Statistical test results obtainedx²= 15.922, p-value 0.003 <0.05 means that there is a relationship between knowledge and sexual behavior in adolescents at SMK MJPS 1 Kota Tasikmalaya. Based on the results of the study, there is a need for education and counseling for adolescents by the local health center regarding the impact of sexual behavior on adolescents.
PENGARUH REBUSAN DAUN SALAM TERHADAP PENURUNAN KADAR ASAM URAT PADA LANSIA: LITERARURE REVIEW
Authors: Patyawargana Pandu Patyawargana , Falah Miftahul 
Published: February 2021

Tingginya angka kejadian penyakit gout artritis di masyarakat yang dapat menimbulkan dampak yang cukup serius. Untuk mencegah atau menurunkan kadar urat meningkat yaitu dengan memanfaatkan tumbuhan alami berupa daun salam. Daun salam mengandung flavonoid yang mampu menurunkan kadar asam urat. Tujuan penelitian ini adalah untuk mengetahui pengaruh rebusan daun salam terhadap penurunan kadar asam urat lansia. Metode penelitian ini adalah literature Review melalui pencarian artikel yang dilakukan di internet dengan search engine Google Scholar dan Portal Garuda, terdiri dari 120 populasi didapat 8 jurnal full text yang sesuai kriteria inklusi. Hasil penelitian bahwa daun salam terbukti efektif menurunkan kadar asam urat pada penderita gout arthitis. Daun salam mempunyai kandungan yang bermanfaat bagi penderita gout arthitis yaitu diantaranya flavonoid. Kesimpulan dari penelitian ini adalah bahwa ada pengaruh rebusan daun salam terhadap penurunan kadar asam urat pada lansia penderita gout artritis. Peneliti ini pun menyarankan dalam melakukan penatalaksanaan penurunan kadar asam urat pada penderita gout artritis dapat melakukan dengan cara mengkonsumsi rebusan daun salam. Dimana hal tersebut sudah terbukti dari penelitian sebelumnya yang telah mendapatkan hasil yang signifikan bernilai memiliki pengaruh.
Determinants of digital transformation of the national economy
Authors: Brechko Oleksandr 
Published: February 2021

Introduction. One of the main directions of ensuring the balanced development of the national economy is the digital transformation, which covers almost all spheres of life at the micro, meso and macro levels. An important component of the digital transformation of the economy is the service provision of its subsystems, which is carried out on the basis of a platform approach that is able to digitize social, economic and technological processes in the construction of ecosystems. In the context of the Fourth Industrial Revolution, digitalization has become a tool for commercializing the achievements of technological progress. This highlights the need to study the key determinants of digital transformation and opportunities for economic breakthrough.The object of research is the process of digital transformation of the constituent subsystems of the national economy.Purpose. The subject of the study is a set of scientific approaches and practical mechanisms for digitization of business processes as a factor of accelerated economic development.Goal. The study is devoted to substantiating the conceptual foundations of the digital transformation of the national economy in the context of its accelerated development.Methods. In the process of research general scientific methods were used: system, structural analysis, grouping in the study of structural elements and identification of factors and problems of digitization; historical analysis to consider the scientific principles and institutional mechanism of the digital transformation of the economy; comparative analysis in assessing the possibilities of implementing the service component of digitalization; economic analysis in assessing the current state of digital transformation of the national economy.Results. The article analyzes the essential determinants of the digital transformation of the economy, establishes the objective need to introduce digital technologies in the real sector. The scientific novelty of the obtained results lies in the substantiation of conceptual approaches to digital transformation by detailing the forms and methods of its implementation in economic processes.The practical significance of the obtained results is that the developed recommendations will be used to create organizational support for the digital transformation of the national economy at different levels of its functioning.Prospects. Prospects for further research are in the plane of the use of innovative mechanisms for digitization of business processes, the introduction of virtual digital assets, the creation of effective decentralized database management systems.
Theoretical and methodological bases of formation of tourist destinations in western Ukrainian border regions
Authors: Brych Vasyl , Kyfyak Olexander 
Published: February 2021

Introduction. Tourist activity in the western Ukrainian border regions is organized in such a way that a tourist destination becomes a key element of the tourism system. However, their small number and some problems with their creation require theoretical and methodological support, which determines the relevance of the scientific article.Purpose and methods. Definition of basic theories, basic scientific approaches and formation of an ideal image of a tourist destination for the western Ukrainian border regions. The methodological basis of the study are general scientific and theoretical methods:– analysis and synthesis - to determine the basic theories and scientific approaches to the formation of tourist destinations;– expert assessment – to establish the main components of tourist destinations and determine their types;– idealization – to form an ideal image of a tourist destination;– induction, deduction – to determine the basic principles of a systematic approach which is based on the consideration of a tourist destination as a whole object.Results. Based on the analysis of theories of local government, the most important theories are identified and their role in the formation of tourist destinations. In particular, the use of the theory of dualism implies that the formation of tourist destinations must take into account the balance of state and local interests and the inadmissibility of the separation of individual management tasks into local and state. The theory of a free community gives the community the right to independently manage its territory, its resources, determine activities, implement advanced technologies, and so on. No less important for the formation of tourist destinations is the general theory of municipal government or socio-economic theory, which is based not on human rights, but economic necessity and practicality.The analysis of the territories of western Ukrainian border regions on the provision of tourist resources, the organization of cross-border cooperation, which helped to determine the main scientific approaches to the formation of tourist destinations and establish the main components of a tourist destination, determine its types by scale and radius.Conclusions. Defining basic theories and scientific approaches to the formation of tourist destinations, creating an ideal image of a tourist destination for the western Ukrainian border regions will contribute to their further successful operation.Discussion. Prospects for further research include the study of foreign experience, the use of a cluster approach, ranking and sequence of actions in the theoretical and methodological support of the formation of tourist destinations.
Ways to improve the innovation performance of enterprises
Authors: Trush Iryna 
Published: February 2021

Introduction. It is proved that the role of innovation efficiency is significant for high- tech enterprises and is growing for the economy as a whole. However, evaluation of the efficiency of investment in innovation, which is used in practice, does not take into account sufficiently the features of the innovation process, which leads to a high proportion of false decisions and lack of the commercial outcome of innovations. To increase the efficiency of innovative activities of enterprises, new requirements are put forward to improve the mechanism of selection of innovative projects during their internal examination.The purpose of the study is to deepen the methodological approaches to assess the effectiveness of innovation performance of enterprises and improve the mechanism of examination of innovative projects.Research methods. The article used general scientific and specific research methods, in particular: analysis and synthesis - to improve the interpretation of the concept of “innovative activity of the enterprise”; system approach - to clarify the economic meaning of the concepts of “management of innovative activities of the enterprise”, “efficiency of innovative activities of the enterprise”; graphic - for visual representation of statistical material and illustration of theoretical and practical provisions; expert assessment - to determine the effectiveness of innovative activities of the enterprise.Results of the research. In the course the evaluation of the efficiency of investments in the innovation project predetermines processing of expert assessments applying special tools, which involves checking the conditions of consistency and consensus of experts’ ideas. The final stage of the expertise involves making a decision on selection of one or several innovation projects for implementation in the conditions of the enterprise under consideration. It is established that the basis of selection should be the rating of the innovation project, the cost of its implementation and the budget of the enterprise’s innovation development. The final decision on the appropriateness of introduction of the innovation project does not require rigid formalization, but should be taken by senior management board of the enterprise as a result of qualitative assessment of the efficiency of investment in innovation projects.Perspectives. In future research, we plan to develop an algorithm for evaluating and selecting innovative projects during its examination at the enterprise level; to investigate the methodological tools for evaluating the effectiveness of innovative activities of enterprises.