bluuebunny/crossref_metadata_2025_split huggingface dataset:
Collaborative control of traffic signal and variable guiding lane for isolated intersection under connected and automated vehicle environment
Authors: Ding Chuan , Dai Rongjian , Fan Yue , Zhang Zhao , Wu Xinkai 
Published: October 2021

AbstractExisting signalized intersection control methods allocate intersection resources to each phase from the time dimension based on traffic conflict. Solving the traffic control issues caused by the unbalanced traffic demands at intersections is always a challenge. Recently developed connected and automated vehicle (CAV) technologies render it possible to collect detailed real‐time traffic information from vehicles for the optimal control and management of time‐space resources at signalized intersections. In this paper, we propose a mixed integer quadratic programming to jointly optimize signal timings and variable guide lane settings for a typical four‐arm intersection in the CAV environment. From the time dimension, restrictions of the conventional concept of the signal cycle are overcome. Phase sequences, green start, and duration are freely assigned for each CAV platoon based on the movement‐based signal timing. From the space dimension, lane resources can be reasonably allocated in consideration of the dynamic traffic demand distribution. In this study, a demand‐response control is realized for the signalized intersection through time‐space resources cooperated optimization. Furthermore, vehicle trajectory control is integrated into the collaborative control framework to reduce or eliminate wasted green time. At last, we propose the dynamic control process using the collaborative control framework. Numerical simulation results show that the collaborative control method outperforms over fixed‐time and signal optimization control modes in terms of travel time with both under‐saturated and over‐saturated conditions.
Detection ofTrypanosoma cruziinfection byPCRinCanis lupus familiarisand their ectoparasites in Chile
Authors: Opazo A. , Bacigalupo A. , Urrutia S. , Chávez G. 
Published: October 2021

AbstractChronic Chagas disease affects humans and animals, involving rural and urban inhabitants. Dogs participate in the maintenance and transmission ofTrypanosoma cruzi. The objective of this study was to evaluate the presence ofT. cruziin dogs and their ticks and fleas, in a rural area of Central Chile.Trypanosoma cruziwas detected by PCR both in dogs and ectoparasites. From the blood samples obtained, 57% were infected byT. cruzi, 5.4% of the ticks detected were positive, and all fleas were negative. Additionally, we performed electrocardiograms and found supraventricular arrhythmia in 44% ofT. cruzi‐positive dogs. Nevertheless, their risk for supraventricular arrhythmias was not higher in infected versus noninfected dogs. Considering the detected infection levels, dogs act asT. cruzihosts in Central Chile, and ticks could be used as an indicator of infection when blood samples are not available. However, at this point, there is no indication that these ticks could pass on the parasite to another host. Periodic ectoparasitic treatment of pets should reduce the chance of vectorial transmission ofT. cruziand improve canine health; however, this is an uncommon practice among rural communities, so governmental programs are encouraged to tackle this problem.
The RNA recognition motif‐containing protein UBA2c prevents early flowering by promoting transcription of the flowering repressor FLM in Arabidopsis
Authors: Zhao Nan , Su Xiao‐Min , Liu Zhang‐Wei , Zhou Jin‐Xing , Su Yin‐Na , Cai Xue‐Wei , Chen Ling , Wu Zhe , He Xin‐Jian 
Published: November 2021

Summary

FLOWERING LOCUS M (FLM) is a well‐known MADS‐box transcription factor that is required for preventing early flowering under low temperatures in Arabidopsis thaliana. Alternative splicing of FLM is involved in the regulation of temperature‐responsive flowering. However, how the basic transcript level of FLM is regulated is largely unknown.

Here, we conducted forward genetic screening and identified a previously uncharacterized flowering repressor gene, UBA2c. Genetic analyses indicated that UBA2c represses flowering at least by promoting FLM transcription.

We further demonstrated that UBA2c directly binds to FLM chromatin and facilitates FLM transcription by inhibiting histone H3K27 trimethylation, a histone marker related to transcriptional repression. UBA2c encodes a protein containing two putative RNA recognition motifs (RRMs) and one prion‐like domain (PrLD). We found that UBA2c forms speckles in the nucleus and that both the RRMs and PrLD are required not only for forming the nuclear speckles but also for the biological function of UBA2c.

These results identify a previously unknown flowering repressor and provide insights into the regulation of flowering time.
Mechanism and potential contributing factors to temporomandibular joint osteoarthritis
Authors: Zhang Juan , Zhang Shuai , Qi Wen‐Jun , Xu Cong‐Lin , Zhou Jie , Wang Jiang‐Hong , Wang Bao‐Li 
Published: October 2021

AbstractObjectiveTo investigate the mechanism of and potential contributing factors to temporomandibular joint osteoarthritis (TMJOA) caused by oestrogen deficiency with a persistent high bite force.Materials and methodsA TMJOA model was generated by subjecting 6‐week‐old female rats to ovariectomy (OVX) and feeding them a hard feed. The rats (n = 12/group) were divided into sham (control); OVX; OVX+hard feed (HF); OVX+hard feed+local‐joint injection of 17β‐oestradiol (an oestrogen) (E2); and OVX+hard feed+local‐joint injection of rapamycin (an autophagy activator) (RAPA)groups. Condyles were stained with haematoxylin‐eosin and Safranin O Fast Green. The expression of Beclin 1, LC3 and p‐mTOR in condylar cartilages was analysed.ResultsTissue staining revealed thinner condylar cartilage, varying numbers or fewer hypertrophic chondrocytes, and lower proteoglycan content in the cartilage matrix of the OVX group. These characteristics were more pronounced in the HF group, but were significantly recovered in the E2 and RAPA groups. Immunohistochemical staining revealed significantly lower autophagic flux in OVX/HF groups and a higher one in E2/RAPA groups.ConclusionsA persistent high bite force could aggravate TMJOA induced by oestrogen deficiency, and the application of oestrogen or rapamycin could delay its progression. Additionally, autophagy may play a role in the development of TMJOA.
Updates of liquid biopsy in oral cancer and multiomics analysis
Authors: Zhang Xinning , Li Binbin 
Published: October 2021

AbstractLiquid biopsy is a method sampled from body fluids, such as blood, saliva, urine, pleural effusion, cerebrospinal fluid, and so on. It is minimally invasive and reproducible and therefore can build a dynamic, real‐time monitoring of oral squamous cell carcinoma patient's conditions and treatment responses. Circulating tumor cells, circulating tumor DNA and exosomes are three main detection objects of liquid biopsy, having different detection methods and features involving cost, sensitivity, specificity and output. Blood and saliva are the options of liquid biopsy in oral cancer. Then we reviewed the studies of liquid biopsy in oral cancer, integrating multiomics analysis of these results. The multiomics analysis of genomics, transcriptomics, proteomics, metabolomics, and DNA methylation have shown potential for the early screening, diagnosis, staging, prognosis, personalized medicine therapy, and monitoring of recurrence (minimal residual disease). Besides, we concluded some problems to be solved, such as the lack of the standard of the measurement methods and procedures of samples, the insufficient connection among different omics, and how to improve the sensitivity and specificity. And we also put up rough assumptions to these problems. However, the analysis of multiomics of liquid biopsy in oral cancer still shows great clinical value in the diagnosis and treatment of oral squamous cell carcinoma.
Effect of tyrosine kinase inhibitor sunitinib on tissue repair at tooth extraction sites
Authors: Ratzkowski Bruna , Koth Valesca S. , Azambuja Alan A. , Salum Fernanda G. , de Figueiredo Maria A. Z. , Cherubini Karen 
Published: November 2021

AbstractBackgroundBoth zoledronic acid, a potent bisphosphonate, and the antiangiogenic drug sunitinib are included in anticancer protocols and have also been associated with jaw osteonecrosis. Our aim was to compare the effect of these drugs on tissue repair at tooth extraction sites.MethodsWistar rats were allocated into four groups: (1) sunitinib; (2) sunitinib/zoledronic acid; (3) zoledronic acid; (4) control group. The animals underwent tooth extractions and maxillae were macro‐ and microscopically analyzed.ResultsOn macroscopic evaluation, the zoledronic acid group showed a significantly higher frequency of oral mucosal lesion; lesions in the sunitinib/zoledronic acid group were larger, albeit not significantly so. The sunitinib/zoledronic acid group had significantly less epithelium than the zoledronic acid and control group, but showed no significant difference compared to the sunitinib group. The sunitinib/zoledronic acid and zoledronic acid groups did not differ from each other, but had significantly less connective tissue and more non‐vital bone and microbial colonies than sunitinib and control groups, whereas these latter two groups did not significantly differ from each other. Vital bone and inflammatory infiltrate did not significantly differ between groups.ConclusionSunitinib alone is not associated with non‐vital bone, whereas the sunitinib/zoledronic acid combination and zoledronic acid alone are.
Food allergy in infants assessed in two German birth cohorts 10 years after the EuroPrevall Study
Authors: Röhrl Daniela V. , Brandstetter Susanne , Siziba Linda P. , Rothenbacher Dietrich , Gaertner Vincent D. , Harner Susanne , Köninger Angela , Apfelbacher Christian , Melter Michael , Genuneit Jon , Kabesch Michael ,   the KUNO Kids Study Group
Published: October 2021

AbstractBackgroundThe prevalence of food allergies (FA) in children increased rapidly at the turn of the century. The EuroPrevall study identified Germany as a country with very high prevalence of FA at that time. Using two large German birth cohorts, we provide an update of the status quo 10 years later.MethodsKUNO Kids and Ulm SPATZ Health studies are two ongoing prospective birth cohorts. Information on FA was obtained by questionnaires at birth and after 6, 12, and 24 months. Univariable and multivariable logistic regression analyses were performed to investigate risk factors during pregnancy, birth, and early childhood.ResultsIn 1139 and 1006 children from KUNO Kids and SPATZ, the point prevalence of parent‐reported FA symptoms at the ages of 1 and 2 years was 13.2% (95% CI: 11.2–15.2) and 13.9% (95% CI: 11.5–17.2) in KUNO Kids. Doctor's diagnosed FA at 1 and 2 years was 2.4% (95% CI: 1.6–3.4) and 2.7% (95% CI: 1.2–4.3) in KUNO Kids and 2.3% (95% CI: 1.3–3.6) and 3% (95% CI: 2.0–4.5) in SPATZ. Cow's milk and citrus fruits were most frequently suspected by parents to cause FA symptoms. Atopy in the child was associated with a higher frequency of FA at any time, whereas atopy in first‐degree relatives was only associated with FA at year 1. Smoke exposure during pregnancy was a risk for FA at age 2.ConclusionThe prevalence of food allergy seems to have plateaued in the last 10 years in Germany. FA is often suspected by parents but only rarely diagnosed by oral food challenge. Risk factor analysis may help to establish personalized health approaches.
Lycopene intake induces colonic regulatory T cells in mice and suppresses food allergy symptoms
Authors: Ushiroda Chihiro , Takagi Tomohisa , Fuke Nobuo , Mizushima Katsura , Hirai Yasuko , Higashimura Yasuki , Harusato Akihito , Kamada Kazuhiro , Uchiyama Kazuhiko , Ishikawa Takeshi , Aizawa Koichi , Suganuma Hiroyuki , Itoh Yoshito , Naito Yuji 
Published: October 2021

AbstractBackgroundFood allergy (FA) is a common disease in children; thus, a high level of safety is required for its prevention and treatment. Colonic regulatory T cells (Tregs) have been suggested to attenuate FA. We investigated the Treg‐inducing ability and anti‐FA effects of carotenoids, a pigment contained in vegetables and fruits.MethodsC57BL/6N mice were fed a diet containing 0.01% (w/w) of lycopene, β‐carotene, astaxanthin or lutein for 4 weeks, and the population of colonic Tregs was assessed. Subsequently, to evaluate the Treg‐inducing ability of lycopene, splenic naïve CD4+ T cells from BALB/c mice were cultured with anti‐CD3/CD28 antibody, TGF‐β and lycopene, and the frequencies of Tregs were examined. The effect of 0.1% (w/w) lycopene containing diet on FA was investigated in OVA‐induced FA model BALB/c mice.ResultsIn screening, only lycopene significantly increased the frequency and number of colonic Tregs. Lycopene also increased Treg differentiation in splenic naïve CD4+ T cells. In FA mice, lycopene feeding significantly increased the number of colonic Tregs and attenuated allergic symptoms. The expression levels of IL‐4, IL‐9 and IL‐13 mRNA in colonic mucosa were also significantly reduced by lycopene. IL‐9 is known to induce proliferation of mast cells, and we found that lycopene feeding significantly reduced the number of mast cells in the colonic mucosa of FA mice.ConclusionOur results suggest that lycopene, a carotenoid present in many common foods on the market, may have the potential to induce colonic Tregs and suppress FA symptoms.
StomataScorer: a portable and high‐throughput leaf stomata trait scorer combined with deep learning and an improved CV model
Authors: Liang Xiuying , Xu Xichen , Wang Zhiwei , He Lei , Zhang Kaiqi , Liang Bo , Ye Junli , Shi Jiawei , Wu Xi , Dai Mingqiu , Yang Wanneng 
Published: October 2021

AbstractTo measure stomatal traits automatically and nondestructively, a new method for detecting stomata and extracting stomatal traits was proposed. Two portable microscopes with different resolutions (TipScope with a 40× lens attached to a smartphone and ProScope HR2 with a 400× lens) are used to acquire images of living stomata in maize leaves. FPN model was used to detect stomata in the TipScope images and measure the stomata number and stomatal density. Faster RCNN model was used to detect opening and closing stomata in the ProScope HR2 images, and the number of opening and closing stomata was measured. An improved CV model was used to segment pores of opening stomata, and a total of 6 pore traits were measured. Compared to manual measurements, the square of the correlation coefficient (R2) of the 6 pore traits was higher than 0.85, and the mean absolute percentage error (MAPE) of these traits was 0.02%–6.34%. The dynamic stomata changes between wild‐type B73 and mutant Zmfab1a were explored under drought and re‐watering condition. The results showed that Zmfab1a had a higher resilience than B73 on leaf stomata. In addition, the proposed method was tested to measure the leaf stomatal traits of other nine species. In conclusion, a portable and low‐cost stomata phenotyping method that could accurately and dynamically measure the characteristic parameters of living stomata was developed. An open‐access and user‐friendly web portal was also developed which has the potential to be used in the stomata phenotyping of large populations in the future.
Hepatic artery reconstruction using an operating microscope in pediatric liver transplantation—Is it worth the effort?
Authors: Dziodzio Tomasz , Martin Friederike , Gül‐Klein Safak , Globke Brigitta , Ritschl Paul Viktor , Jara Maximilian , Hillebrandt Karl‐Herbert , Nösser Maximilian , Koulaxouzidis Georgios , Fehrenbach Uli , Gratopp Alexander , Henning Stephan , Bufler Philipp , Schöning Wenzel , Schmelzle Moritz , Pratschke Johann , Witzel Christian , Öllinger Robert 
Published: November 2021

AbstractIntroductionIn pediatric liver transplantation (pLT), hepatic artery thrombosis (HAT) is associated with inferior transplant outcome. Hepatic artery reconstruction (HAR) using an operating microscope (OM) is considered to reduce the incidence of HAT.MethodsHAR using an OM was compared to a historic cohort using surgical loupes (SL) in pLT performed between 2009 and 2020. Primary endpoint was the occurrence of HAT. Secondary endpoints were 1‐year patient and graft survival determined by Kaplan–Meier analysis and complications. Multivariate analysis was used to identify independent risk factors for HAT and adverse events.ResultsA total of 79 pLTs were performed [30 (38.0%) living donations; 49 (62.0%) postmortem donations] divided into 23 (29.1%) segment 2/3, 32 (40.5%) left lobe, 4 (5.1%) extended right lobe, and 20 (25.3%) full‐size grafts. One‐year patient and graft survival were both 95.2% in the OM group versus 86.2% and 77.8% in the SL group (p = .276 and p = .077). HAT rate was 0% in the OM group versus 24.1% in the SL group (p = .013). One‐year patient and graft survival were 64.3% and 35.7% in patient with HAT, compared to 93.9% and 92.8% in patients with no HAT (both p < .001). Multivariate analysis revealed HAR with SL (p = .022) and deceased donor liver transplantation (DDLT) (p = .014) as independent risk factors for HAT. The occurrence of HAT was independently associated with the need for retransplantation (p < .001) and biliary leakage (p = .045).ConclusionIn pLT, the use of an OM is significantly associated to reduce HAT rate, biliary complications, and graft loss and outweighs the disadvantages of delayed arterial perfusion and prolonged warm ischemia time (WIT).
Seed viability and fatty acid profiles of five orchid species before and after ageing
Authors: Diantina S. , McGill C. , Millner J. , Nadarajan J. , Pritchard H. W. , Colville L. , Clavijo McCormick A. 
Published: November 2021

Abstract

Changes in seed lipid composition during ageing are associated with seed viability loss in many plant species. However, due to their small seed size, this has not been previously explored in orchids. We characterized and compared the seed viability and fatty acid profiles of five orchid species before and after ageing: one tropical epiphytic orchid from Indonesia (Dendrobium strebloceras), and four temperate species from New Zealand, D. cunninghamii (epiphytic), and Gastrodia cunninghamii, Pterostylis banksii and Thelymitra nervosa (terrestrial).

Seeds were aged under controlled laboratory conditions (3‐month storage at 60% RH and 20 °C). Seed viability was tested before and after ageing using tetrazolium chloride staining. Fatty acid methyl esters from fresh and aged seeds were extracted through trans‐esterification, and then analysed using gas chromatography–mass spectrometry.

All species had high initial viability (>80%) and experienced significant viability loss after ageing. The saturated, polyunsaturated, monounsaturated and total fatty acid content decreased with ageing in all species, but this reduction was only significant for D. strebloceras, D. cunninghamii and G. cunninghamii.

Our results suggest that fatty acid degradation is a typical response to ageing in orchids, albeit with species variation in magnitude, but the link between fatty acid degradation and viability was not elucidated. Pterostylis banksii exemplified this variation; it showed marked viability loss despite not having a significant reduction in its fatty acid content after ageing. More research is required to identify the effect of ageing on fatty acid composition in orchids, and its contribution to seed viability loss.
Psychophysiological correlates of pain resilience in anticipating, experiencing, and recovering from pain
Authors: Li Fenghua , Jackson Todd 
Published: October 2021

AbstractAlthough researchers have documented behavioral and brain structure correlates of pain resilience, associated physiological responses have received little consideration. In this study, we assessed psychophysiological differences between high (HPR), moderate (MPR), and low (LPR) pain resilience subgroups during anticipation, experiencing, and recovery from laboratory pain. In an initial pain anticipation task, participants (79 women, 32 man) viewed visual cues to signal possible mild or intense shocks prior to receiving these shocks. Subsequently, in a pain recovery task, participants received uncued mild and intense shocks. Subjective appraisals were assessed during each task in tandem with continuous recording of skin conductance level (SCL), heart rate variability (HRV), and corrugator electromyography (cEMG). On physiological indexes, HPR subgroup members displayed significantly lower SCL than MPR and LPR subgroups did during anticipation and experiencing of pain while no resilience group effects were found for HRV or cEMG. During pain recovery, HPR and LPR subgroups displayed weaker SCL than the MPR subgroup did in the immediate aftermath of shock. However, HPR members continued to display lower SCL than other groups did over an extended recovery period. On self‐report measures, the LPR subgroup reported higher levels of anticipatory anxiety and expected pain than HPR and MPR subgroups did during the pain anticipation task. Together, results suggested higher pain resilience is characterized, in part, by comparatively reduced SCL during the course of anticipating, experiencing and recovering from painful shock.
MicroRNA840 (MIR840) accelerates leaf senescence by targeting the overlapping 3′UTRs of PPR and WHIRLY3 in Arabidopsis thaliana
Authors: Ren Yujun , Li Mengsi , Wang Wanzhen , Lan Wei , Schenke Dirk , Cai Daguang , Miao Ying 
Published: November 2021

SUMMARYMicroRNAs negatively regulate gene expression by promoting target mRNA cleavage and/or impairing its translation, thereby playing a crucial role in plant development and environmental stress responses. In Arabidopsis, the MIR840 gene is located within the overlapping 3′UTR of the PPR and WHIRLY3 (WHY3) genes, both being predicted targets of miR840* and miR840, the short maturation products of MIR840. Gain‐ and loss‐of‐function of MIR840 in Arabidopsis resulted in opposite senescence phenotypes. The highest expression levels of the MIR840 precursor transcript pre‐miR840 were observed at senescence initiation, and pre‐miR840 expression is significantly correlated with a reduction in PPR, but not WHY3, transcript levels. Although a reduction of transcript level of PPR, but not WHY3 transcript levels were not significantly affected by MIR840 overexpression, its protein levels were strongly reduced. Mutating the cleavage sites or replacing the target sequences abolishes the miR840*/miR840‐mediated degradation of PPR transcripts and accumulation of WHY3 protein. In support for this, concurrent knockdown of both PPR and WHY3 in wild‐type plants resulted in a senescence phenotype resembling that of the MIR840‐overexpressing plant. This indicates that both PRR and WHY3 are targets in the MIR840‐mediated senescence pathway. Moreover, single knockout mutants of PPR and WHY3 show a convergent upregulated subset of senescence‐associated genes, which are also found among those induced by MIR840 overexpression. Our data provide evidence for a regulatory role of MIR840 in plant senescence.