bluuebunny/crossref_metadata_2025_split huggingface dataset:
Prussian Blue and Polyvinylpyrrolidone‐Embedded Hollow Fiber Gradient Polysulfone Membrane for Uric Acid Biosensor Application
Authors: Wang Yi‐Kun , Chen Ya‐Jun , Pan Yao , Hai‐Yin Yu 
Published: December 2024

ABSTRACTTraditional biosensors have drawbacks such as temperature sensitivity and cross‐reactivity. An innovative UA biosensor was developed. It combined Prussian Blue, carbon nanotubes, PVP with uricase oxidase in a polysulfone hollow fiber gradient membrane, forming a multilayer 3D structure. The optimal pH and temperature for the biosensor were determined to be 8.0 and 37°C, respectively. Electrochemical impedance spectroscopy (EIS) showed that the nanoparticles significantly reduced the working electrode's impedance, enhancing electron transfer. Cyclic voltammetry (CV) and differential pulse voltammetry (DPV) were used to study the electrochemical behavior of UA oxidation. A distinct oxidation peak for UA was observed at 0.7 V. The sensor exhibited a sensitivity of 0.33 × 10−6 A/mM, a linear working range from 1.2 to 12 mM, and a detection limit of 0.424 mM. The porous structure of the membrane and the nanoparticles' synergistic effect contributed to high sensor stability. After continuous use for a week, the sensor maintained approximately 90% of its initial performance. This research demonstrates the effectiveness of our method in developing highly sensitive and stable biosensors for UA detection, providing a promising solution for accurate and reliable uric acid level monitoring.
Sunlight Self‐Heating Oil‐Water Separation Material and Device for Recovering High‐Viscosity Oil
Authors: Wang Zhuqing , Li Yiyang , Meng Yupu , Huang Zhiwei , Wang Min 
Published: December 2024

ABSTRACTThis research employed a simplistic approach to modify polydimethylsiloxane (PDMS) and graphene oxide (GO) onto the surface of the PU skeleton, and then synthesize an oil–water separation material (rGO@PDMS/PU) with photothermal conversion performance and superoleophilic/superhydrophobic properties through high‐temperature reduction. The synthesized rGO@PDMS/PU can adsorb organic solvents or oil that are 19–37 times its own weight, while also having a 99.1% oil–water separation efficiency. Additionally, rGO@PDMS/PU also has good photothermal conversion ability. Under the intensity of 1 sunlight (q = 1 kW m−2), its surface temperature can reach 95.4°C within 10 s. Relying on the excellent oil–water selectivity and photothermal conversion capabilities of rGO@PDMS/PU, we also build a novel oil‐gathering device that can successfully make oil recovery on the water surface in situ. When the device is under light conditions, its crude oil recovery efficiency is nearly four times higher than when there is no light. Therefore, this new oil‐gathering device has broad application prospects in high‐viscosity oil leakage incidents such as crude oil and engine oil.
Germinative dynamic, seasonality, and polyandry: A dive in neotropical point‐belly frog reproduction
Authors: Bordin Rafael O. A. , de Oliveira Classius , Domeniconi Raquel F. 
Published: December 2024

AbstractComplexity and reproductive plasticity are very well‐known characteristics of anurans. The group is being continuously explored taxonomically and phylogenetically, which leads to a great potential diversity not yet uncovered. This is particularly evident due to the lack of investigation regarding species‐specific traits, even the ones well distributed in the lineages as polyandry. Although the climate and environment affect reproductive variability, each species has unique dynamics, and a deeper understanding of their reproductive cycles is essential for meaningful comparisons and recognition of patterns within the extensive variability observed in anurans. Given this context, the present study examined the reproductive and environmental parameters of the polyandrous species Leptodactylus podicipinus throughout its reproductive cycle over two consecutive years. The objective was to characterize the species' reproductive dynamics and compare them with phylogenetically related species and other polyandrous species, aiming to explore patterns and the influence of polyandry on anuran reproduction. The main reproductive activity of L. podicipinus was during the months with greater rainfall, from October to February. The seminiferous epithelium presented a higher mean of final germinative cells (spermatozoa) in the reproductive period (October–February) and early germinative cells (spermatocytes I and spermatids) in the non‐reproductive period (March–September). Among the climatic parameters tested, rainfall was the only one significantly related to reproductive outcomes. Two distinct germinative patterns were identified between polyandric and non‐polyandric species compared. Polyandry is certainly a trait that plays a significant role in shaping gametogenesis and the evolution of animals with this reproductive attribute.
Predictions to Increase Lasso Peptide Production in the Heterologous Host Streptomyces coelicolor M1152
Authors: Razmilic Valeria , Asenjo Juan A. , Martínez Irene 
Published: December 2024

ABSTRACTProduction of specialized metabolites are restricted to the metabolic capabilities of the organisms. Genome‐scale models (GEM)s are useful to study the whole metabolism and to find metabolic engineering targets to increase the yield of a target compound. In this work we use a modified model of Streptomyces coelicolor M145 to simulate the production of lagmysin A (LP4) and the novel lagmysin B (LP2) lasso peptide, in the heterologous host Streptomyces coelicolor M1152. Overexpression targets were identified using the flux scanning based on enforced objective flux (FSEOF) algorithm and flux variability analysis (FVA), considering growth in minimum and in complex medium. Thirteen reactions were found as candidate metabolic engineering targets for both lasso peptides considering both settings. We propose the overexpression of enzymes of the glycolysis pathway (GAPD, PGK, PGM and ENO) and leucine biosynthesis (IPPS, IPPMIb, IPPMIa, IPMD and OMCDC) to enhance the production of either lagmysin A or B.
Corporate Anti‐Corruption Disclosure and Corporate Sustainability Performance in the United Kingdom: Does Sustainability Governance Matter?
Authors: Gerged Ali Meftah , Salem Rami , Ghazwani Musa 
Published: December 2024

ABSTRACTThis research investigates the potential effects of companies' commitments to disclose their anti‐corruption efforts on their sustainability performance. Additionally, we aim to analyze whether the existence of a sustainability committee influences this relationship. To achieve these objectives, we gathered data from 5344 firm‐year observations of companies listed on the FTSE 350 index from 2008 to 2023. Our findings provide strong empirical support for a positive relationship between companies' anti‐corruption disclosures and their sustainability performance. Furthermore, our evidence suggests that the presence of a sustainability committee acts as a viable complement to anti‐corruption disclosures, driving improved sustainability performance. Our study highlights practical implications for organizations, regulators, and policymakers, and it opens avenues for future research.
Turn TRAIL Into Better Anticancer Therapeutic Through TRAIL Fusion Proteins
Authors: Wang Yan , Qian Xin , Wang Yubo , Yu Caiyuan , Feng Li , Zheng Xiaoyan , Wang Yaya , Gong Qiuhong 
Published: December 2024

ABSTRACTBackgroundTNF‐related apoptosis‐inducing ligand (TRAIL) belongs to the tumor necrosis factor superfamily. TRAIL selectively induces apoptosis in tumor cells while sparing normal cells, which makes it an attractive candidate for cancer therapy. Recombinant soluble TRAIL and agonistic antibodies against TRAIL receptors have demonstrated safety and tolerability in clinical trials. However, they have failed to exhibit expected clinical efficacy. Consequently, extensive research has focused on optimizing TRAIL‐based therapies, with one of the most common approaches being the construction of TRAIL fusion proteins.MethodsAn extensive literature search was conducted to identify studies published over the past three decades related to TRAIL fusion proteins. These various TRAIL fusion strategies were categorized based on their effects achieved.ResultsThe main fusion strategies for TRAIL include: 1. Construction of stable TRAIL trimers; 2. Enhancing the polymerization capacity of soluble TRAIL; 3. Increasing the accumulation of TRAIL at tumor sites by fusing with antibody fragments or peptides; 4. Decorating immune cells with TRAIL; 5. Prolonging the half‐life of TRAIL in vivo; 6. Sensitizing cancer cells to overcome resistance to TRAIL treatment.ConclusionThis work focuses on the progress in recombinant TRAIL fusion proteins and aims to provide more rational and effective fusion strategies to enhance the efficacy of recombinant soluble TRAIL, facilitating its translation from bench to bedside as an effective anti‐cancer therapeutic.
Cardiovascular and Metabolic Adverse Events of Endocrine Therapies in Women with Breast Cancer: A Disproportionality Analysis of Reports in the FDA Adverse Event Reporting System
Authors: Elshafie Shaimaa , Villa‐Zapata Lorenzo , Tackett Randall L. , Zaghloul Iman Y. , Young Henry N. 
Published: December 2024

ABSTRACTIntroductionEmerging evidence suggests potential cardiovascular toxicities from oral endocrine therapies (ETs); however, results are conflicting. This study comprehensively examined adverse reactions of ETs and investigated cardiovascular and metabolic safety signals within the FDA Adverse Event Reporting System (FAERS).MethodsReports in the FAERS through December 2023 were analyzed for documented reactions to tamoxifen, letrozole, anastrozole, and exemestane in female breast cancer patients. Standardized queries were used to identify cases of cardiovascular (myocardial infarction, heart failure, arrhythmia, stroke) and metabolic (hypertension, dyslipidemia, hyperglycemia) disorders. Descriptive and disproportionality analyses were performed to assess reports and detect safety signals.ResultsAmong 14,327 unique ET‐related reports, arthralgia (n = 1873 events) was the most prevalent reaction. We identified 2170 cardiovascular and 2252 metabolic events associated with ETs. Letrozole had the highest reporting rate of cardiac arrhythmia (7.7%) and showed positive signals for both arrhythmia (reporting odds ratio [ROR] = 2.2; 95% confidence interval [CI]: 1.8–2.5) and myocardial infarction (ROR = 1.9; 95% CI: 1.4–2.6). We also observed a significantly increased risk of heart failure with letrozole (ROR = 1.3; 95% CI: 1.1–1.6) and stroke with tamoxifen (ROR = 1.7; 95% CI: 1.5–2.1). Only anastrozole was significantly associated with metabolic dysfunctions with a notable hyperglycemia reporting rate of 12.2%.ConclusionOur findings provide valuable evidence on common reactions as well as controversial cardiovascular and metabolic abnormalities associated with the real‐world use of ETs for breast cancer. Ongoing benefit–risk assessment and close monitoring of cardiac function during treatment, particularly in high‐risk women, are warranted to optimize cancer outcomes while minimizing cardiovascular injury.
Immune Cell Profiling Reveals a Common Pattern in Premetastatic Niche Formation Across Various Cancer Types
Authors: Sugiyama Shigeaki , Yumimoto Kanae , Nakayama Keiichi I. 
Published: December 2024

ABSTRACTBackgroundMetastasis is the major cause of cancer‐related mortality. The premetastatic niche is a promising target for its prevention. However, the generality and cellular dynamics in premetastatic niche formation have remained unclear.AimsThis study aimed to elucidate the generality and cellular dynamics in premetastatic niche formation.Materials and MethodsWe performed comprehensive flow cytometric analysis of lung and peripheral immune cells at three time points (early premetastatic, late premetastatic, and micrometastatic phases) for mice with subcutaneous implants of three types of cancer cells (breast cancer, lung cancer, or melanoma cells). The immuno‐cell profiles were then used to predict the metastatic phase by machine learning.ResultsWe found a common pattern of changes in both lung and peripheral immune cell profiles across the three cancer types, including a decrease in the proportion of eosinophils in the early premetastatic phase, an increase in that of regulatory T cells in the late premetastatic phase, and an increase in that of polymorphonuclear myeloid‐derived suppressor cells and a decrease in that of B cells in the micrometastatic phase. Machine learning using immune cell profiles could predict the metastatic phase with approximately 75% accuracy.DiscussionValidation of our findings in humans will require data on the presence or absence of micrometastases in patients and the accumulation of comprehensive and temporal information on immune cells. In addition, blood proteins, extracellular vesicles, DNA, RNA, or metabolites may be useful for more accurate prediction.ConclusionThe discovery of generalities in premetastatic niche formation allow prediction of metastatic phase and provide a basis for the development of methods for early detection and prevention of cancer metastasis in a cancer type‐independent manner.
Novel Cinnamic Acid Derivatives Containing Naproxen as NLRP3 Inhibitors: Synthesis and Evaluation of Their Biological Activity
Authors: Yu Jialin , You Zonglin , Wong Vincent Kam Wai , Chen Min , Liu Wenfeng 
Published: December 2024

ABSTRACTLong‐term use of naproxen can lead to serious side effects. Inspired by the biological activity of cinnamic acid, a series of cinnamic acid derivatives containing naproxen were designed and synthesized, and their anti‐inflammatory activities and mechanisms were explored in vitro. Our results indicated that all of naproxen derivatives showed more significant inhibition against lipopolysaccharide (LPS)‐induced nitric oxide (NO) production and had a lower degree of cytotoxicity than that of naproxen. The present studies revealed that compound 23 (IC50 = 5.66 ± 1.66 µM) markedly inhibited the LPS‐induced NO production and the over‐expression of pro‐inflammatory cytokines, including interleukin (IL)‐1β, inducible NO synthase (iNOS), and cyclooxygenase‐2 (COX‐2). Furthermore, it blocked the activation of NF‐κB signaling pathway and pyrin domain‐containing protein 3 (NLRP‐3) inflammasome in a concentration‐dependent manner. Additionally, docking studies confirmed that compound 23 exhibited a well‐fitting into the NLRP3 active site. Considering these results, compound 23 might be a novel NLRP3 inhibitor to treat inflammatory diseases.
Shh Protects the Injured Spinal Cord in Mice by Promoting the Proliferation and Inhibiting the Apoptosis of Nerve Cells via the Gli1–TGF–β1/ERK Axis
Authors: Liu Yunfei , Hou Meihua , Wang Jingran , Li Xiaoying , Zhang Bichao , Lin Juntang , Yang Ciqing 
Published: December 2024

ABSTRACTSpinal cord injury (SCI) is a common neurological trauma that cannot be completely cured with surgical techniques and medications. In this study, we established a mouse SCI model and used an adeno‐associated virus (AAV) to achieve the high expression of sonic hedgehog (Shh) at the injury site to further investigate the therapeutic effect and mechanism of Shh on SCI. The results of the present study show that Shh may promote motor function recovery. The present findings demonstrate the protective effect of Shh overexpression in SCI by regulating the proliferation and apoptosis of nerve cells at the site of SCI. Shh promotes the proliferation of early microglia, inhibits the proliferation of early astrocytes, and promotes the formation of neurons at the site of injury. In addition, Shh may inhibit apoptosis at the SCI site. The mechanism by which Shh regulates nerve cells at the site of SCI may involve glioma‐associated oncogene 1 (Gli1). The present research indicates that Gli1 regulates the transforming growth factor‐β (TGF‐β) signaling pathway, inhibiting the classic TGF‐β1/Smad signaling pathway and activating the TGF‐β1/extracellular regulated protein kinase (ERK) signaling pathway. Collectively, these findings suggest that Shh is a regulatory molecule involved in nerve cell proliferation and apoptosis. High Shh expression can accelerate motor function recovery after SCI, indicating that it may be a promising therapeutic approach for SCI.
High amylose maize starch can produce an inclusion complex with extract of medicinal plants of Amphipterygium adstringent
Authors: Moreno‐Zaragoza Josue , Pecina‐Ornelas Daniela F. , Agama‐Acevedo Edith , Rosell Cristina M. , Bello‐Pérez Luis Arturo 
Published: December 2024

AbstractBackground and ObjectiveThe interaction between starch and polyphenol would increase the bioaccessibility of those bioactive compounds. Previous studies have been conducted with pure polyphenols, but the use of medicinal plants, rich in bioactive compounds, able to complex with starch, is growing. The present study aimed to analyze the complex formation between the extract of a medicinal plant (Amphipterygium adstringent, AA) and high amylose starch (HA). The total polyphenol content (TPC), and antioxidant capacity (AC), besides X‐ray diffraction pattern, differential scanning calorimetry, Fourier transform infrared spectroscopy (FTIR), and starch hydrolysis rate were evaluated in the AA‐HA and compared with those of the complex gallic acid (GA)‐HA.FindingsThe AA‐HA complex had higher TPC than GA‐HA, despite their rather similar AC. The increased crystallinity and short‐range order of the HA confirmed the formation of the complex with AA. This complex required higher enthalpy (2.0 J/g) than its respective HA control (0.52 J/g) for the gelatinization and the in vitro starch hydrolysis suggests the formation of resistant starch.ConclusionThe extract of medicinal plants can form complexes with amylose, which has antioxidant properties, besides increasing the resistant starch.Significance and NoveltyComplex formation between HA and extract of medicinal plants is feasible for increasing the release of the bioactive compounds after digestion.
Grimontia hollisae Sepsis in a 9‐Month‐Old Female Infant: A Case Report
Authors: Yusuf Abdulsalam Olawale , Isaac Osei , Baleng Mahama Wutor , Adefila Williams Oluwatosin , Lamin Keita Modou , Hossain Ilias , Molfa Minteh , Barjo Ousman , Omotosho Mayowa Banke , Salaudeen Rasheed , Mackenzie Grant 
Published: December 2024

ABSTRACTGrimontia hollisae, an uncommon cause of sepsis, was identified in a 9‐month‐old infant in Africa without confirmed seafood consumption. Prompt diagnosis through blood culture and targeted antibiotic therapy ensured recovery, emphasizing the need for increased awareness, enhanced diagnostic tools, and active monitoring of emerging pathogens in tropical and resource‐limited regions. We present a case report involving a 9‐month‐old infant who exhibited symptoms of acute gastroenteritis. The blood culture revealed
G. hollisae
. We treated the infant on empirical first‐line antibiotics of IV ceftriaxone, IV gentamicin, zinc tablets, and syrup paracetamol for 5 days after which the child was discharged on oral metronidazole following resolution of symptoms. This case highlights the importance of
G. hollisae
in causing sepsis in infants in tropical settings. It also emphasizes the need for blood culture investigation and administering the appropriate antibiotics in the diagnosis of children presenting with suspected sepsis.
Numbness on the Medical Take: An Atypical Presentation of Guillain‐Barre Syndrome With Unilateral Paraesthesia and Rapid Progression to Bulbar Palsy: A Case Report
Authors: Stanley J. , Zhou Q. 
Published: December 2024

ABSTRACTGuillain‐Barré syndrome (GBS) is characterized classically by progressive and symmetrical motor weakness and areflexia. We describe a case of GBS with initially preserved reflexes and power, leading to delayed diagnosis, who latterly required urgent ventilator support and plasmapharesis to highlight the importance of considering atypical presentations of this common condition.
Unravelling the Relaxation Pathway and Excited State Dynamics of Ruthenium(II) Polypyridyl Complexes Incorporating Phosphorus‐Based Monodentate Ligands
Authors: Mishra Ramranjan , Saha Abhijit , Chatterjee Pritha , Kundu Sucheta , Verma Madhu , Sarkar Sabyasachi , Sivakumar Sri , Datta Anindya , Patra Ashis K. 
Published: December 2024

AbstractHerein, the photophysical, photochemical properties and photogenerated excited state dynamics of two new Ru(II) complexes, viz. [Ru(p‐ttp)(bpy)(PTA)]2+ [1]2+, [Ru(p‐ttp)(phen)(PTA)]2+ [2]2+ having a phosphorus‐based ligand PTA [p‐ttp=p‐tolyl terpyridine; bpy=2,2’‐bipyridyl; phen=1,10‐phenanthroline and PTA=1,3,5‐triaza‐7‐phosphaadamantane] are reported. Upon excitation with 470 nm LED, [1]2+ and [2]2+ neither undergo ligand release nor exhibit room temperature luminescence/1O2 generation. However, when excited around their respective MLCT bands at 77 K, luminescence bands at ~600 nm were observed. N‐methylation of the coordinated PTA in complex [1]2+ resulted in [Ru(p‐ttp)(bpy)(MePTA]3+ {[1‐Me]3+} (MePTA=N‐methyl‐1,3,5‐triaza‐7‐phosphaadamantane). The differences in the photophysical and electrochemical properties of [1]2+ and [1‐Me]3+ were rationalized by DFT calculations. The transient absorption spectroscopic analysis was used to unravel the relaxation pathway of the complexes, suggesting the involvement of the 3MC state in the relaxation process. The effect of electronic modulation resulted from the N‐methylation of PTA on the excited state dynamics of the complexes was observed. The MTT assays in various cancer cells revealed their non‐cytotoxic nature in the dark or upon irradiation with 470 nm blue LED. The non‐toxicity of the complexes, despite being internalized in cancer cells as established by ICP‐MS, is attributed to their dark and photostability and the inability to generate 1O2.