bluuebunny/crossref_metadata_2025_split huggingface dataset:
An unprecedented occult non-communicating rudimentary uterine horn treated with laparoscopic excision and preservation of both fallopian tubes: a case report and review of the literature
Authors: Gitas G. , Eckhoff K. , Rody A. , Ertan A. K. , Baum S. , Hoffmans E. , Alkatout I. 
Published: February 2021

Abstract
Background
Müllerian duct anomalies are congenital malformations of the female genital tract and may be of various types. For decades they have been classified according to the American Society of Reproductive Medicine, which mentions unicornuate uterine malformations as the second subgroup. They result from the arrested development of one of the Müllerian ducts and appear in approximately 1/1000 women. These anomalies are usually diagnosed in the second decade of life, because they tend to remain asymptomatic until adolescence and their initial symptoms may vary. Patients present with symptoms such as dysmenorrhea, infertility, and chronic or acute abdominal pain.

Case presentation
We report on a 21-year-old Caucasian German patient who suffered from dysmenorrhea for 7 years. After a transvaginal ultrasound and magnetic resonance tomography of the pelvis was performed, the patient underwent a diagnostic hysteroscopy and operative laparoscopy, and was finally diagnosed with a Müllerian duct anomaly presenting with a non-communicating rudimentary uterine horn. The left tube arose directly in orthotopic location from the cornua of uterus, with no connection to the rudimentary uterine horn or structure.

Conclusion
The anatomic features of this case have not been reported previously and were not consistent with any existing classification. More cases are needed in order to confirm our hypothesis. Gynecologists should always consider Müllerian anomalies as an important differential diagnosis in young patients with abdominal pain.
CD10 marks non-canonical PPARγ-independent adipocyte maturation and browning potential of adipose-derived stem cells
Authors: Chakraborty Smarajit , Ong Wee Kiat , Yau Winifred W. Y. , Zhou Zhihong , Bhanu Prakash K. N. , Toh Sue-Anne , Han Weiping , Yen Paul M. , Sugii Shigeki 
Published: February 2021

Abstract
Background
Effective stem cell therapy is dependent on the stem cell quality that is determined by their differentiation potential, impairment of which leads to poor engraftment and survival into the target cells. However, limitations in our understanding and the lack of reliable markers that can predict their maturation efficacies have hindered the development of stem cells as an effective therapeutic strategy. Our previous study identified CD10, a pro-adipogenic, depot-specific prospective cell surface marker of human adipose-derived stem cells (ASCs). Here, we aim to determine if CD10 can be used as a prospective marker to predict mature adipocyte quality and play a direct role in adipocyte maturation.

Methods
We first generated 14 primary human subject-derived ASCs and stable immortalized CD10 knockdown and overexpression lines for 4 subjects by the lentiviral transduction system. To evaluate the role of CD10 in adipogenesis, the adipogenic potential of the human subject samples were scored against their respective CD10 transcript levels. Assessment of UCP1 expression levels was performed to correlate CD10 levels to the browning potential of mature ASCs. Quantitative polymerase chain reaction (qPCR) and Western blot analysis were performed to determine CD10-dependent regulation of various targets. Seahorse analysis of oxidative metabolism and lipolysis assay were studied. Lastly, as a proof-of-concept study, we used CD10 as a prospective marker for screening nuclear receptor ligands library.

Results
We identified intrinsic CD10 levels as a positive determinant of adipocyte maturation as well as browning potential of ASCs. Interestingly, CD10 regulates ASC’s adipogenic maturation non-canonically by modulating endogenous lipolysis without affecting the classical peroxisome proliferator-activated receptor gamma (PPARγ)-dependent adipogenic pathways. Furthermore, our CD10-mediated screening analysis identified dexamethasone and retinoic acid as stimulator and inhibitor of adipogenesis, respectively, indicating CD10 as a useful biomarker for pro-adipogenic drug screening.

Conclusion
Overall, we establish CD10 as a functionally relevant ASC biomarker, which may be a prerequisite to identify high-quality cell populations for improving metabolic diseases.
Cocktail of carbohydrases from Aspergillus niger: an economical and eco-friendly option for biofilm clearance from biopolymer surfaces
Authors: Kaur Arashdeep , Soni Sanjeev Kumar , Vij Shania , Rishi Praveen 
Published: February 2021

AbstractBiofilm formation on both biotic and abiotic surfaces accounts for a major factor in spread of antimicrobial resistance. Due to their ubiquitous nature, biofilms are of great concern for environment as well as human health. In the present study, an integrated process for the co-production of a cocktail of carbohydrases from a natural variant of Aspergillus niger was designed. The enzyme cocktail was found to have a noteworthy potential to eradicate/disperse the biofilms of selected pathogens. For application of enzymes as an antibiofilm agent, the enzyme productivities were enhanced by statistical modelling using response surface methodology (RSM). The antibiofilm potential of the enzyme cocktail was studied in terms of (i) in vitro cell dispersal assay (ii) release of reducing sugars from the biofilm polysaccharides (iii) the effect of enzyme treatment on biofilm cells and architecture by confocal laser scanning microscopy (CLSM). Potential of the enzyme cocktail to disrupt/disperse the biofilm of selected pathogens from biopolymer surfaces was also assessed by field emission scanning electron microscopy (FESEM) analysis. Further, their usage in conjunction with antibiotics was assessed and it was inferred from the results that the use of enzyme cocktail augmented the efficacy of the antibiotics. The study thus provides promising insights into the prospect of using multiple carbohydrases for management of heterogeneous biofilms formed in natural and clinical settings.
A mathematical model for fall armyworm management on maize biomass
Authors: Daudi Salamida , Luboobi Livingstone , Kgosimore Moatlhodi , Kuznetsov Dmitry , Mushayabasa Steady 
Published: February 2021

AbstractFall armyworm (Spodoptera frugiperda), a highly destructive and fast spreading agricultural pest native to North and South America, poses a real threat to global food security. In this paper, to explore the dynamics and implications of fall armyworm outbreak in a field of maize biomass, we propose a new dynamical system for maize biomass and fall armyworm interaction via Caputo fractional-order operator, which is not only a nonlocal operator but also contains all characteristics concerned with memory of the dynamical system. We define the basic reproduction number, which represents the average number of newborns produced by one individual female moth during its life span. We establish that the basic reproduction number is a threshold quantity, which determines persistence and extinction of the pest. Finally, we simulate the Caputo system using the Adam–Bashforth–Moulton method to illustrate the main results.
Complete and incomplete Bell polynomials associated with Lah–Bell numbers and polynomials
Authors: Kim Taekyun , Kim Dae San , Jang Lee-Chae , Lee Hyunseok , Kim Han-Young 
Published: February 2021

AbstractThe nth r-extended Lah–Bell number is defined as the number of ways a set with $n+r$
n
+
r
elements can be partitioned into ordered blocks such that r distinguished elements have to be in distinct ordered blocks. The aim of this paper is to introduce incomplete r-extended Lah–Bell polynomials and complete r-extended Lah–Bell polynomials respectively as multivariate versions of r-Lah numbers and the r-extended Lah–Bell numbers and to investigate some properties and identities for these polynomials. From these investigations we obtain some expressions for the r-Lah numbers and the r-extended Lah–Bell numbers as finite sums.
Policy-makers’ views on translating burden of disease estimates in health policies: bridging the gap through data visualization
Authors: Lundkvist Amelia , El-Khatib Ziad , Kalra Nikhila , Pantoja Tomas , Leach-Kemon Katherine , Gapp Christian , Kuchenmüller Tanja 
Published: February 2021

Abstract
Background
Knowledge Translation (KT) and data visualization play a vital role in the dissemination of data and information to improve healthcare systems. A better understanding of KT and its utility requires examining its processes, and how these interact with available data tools and platforms and various users.
In this context, the aim of this paper is to understand how relevant users interact with data visualization tools, in particular Global Burden of Disease (GBD) visualizations, while also examining KT processes related to data visualization.

Methods
A qualitative case-study consisting of semi-structured interviews with eight policy officers. Interviewees were selected by the Institute for Health Metrics and Evaluation (IHME) from three countries: Canada, Kenya and New Zealand. Data were analyzed through framework coding, using qualitative analysis software.

Results
Policy officers’ responses indicated that data can prompt action by engaging users, and effective delivery and translation of data was enhanced by data visualization tools. GBD was considered valuable for use in policy (e.g., planning and prioritizing health policy; facilitating accountability; and tracking and monitoring progress and trends over time and between countries). Using GBD and data visualization tools, participants quickly and easily accessed key information and turned it into actionable messages; engaging visuals captured decision-makers’ attention while providing information in a digestible, time-saving manner. However, participants emphasized an overall disconnect between research and public health. Functionality and technical issues, e.g., absence of tool guides and tool complexity, as well as lacking buy-in and awareness of certain tools from those less familiar with GBD, were named as major barriers. In order to address this “know-do” gap, user-friendly knowledge translation tools were stated as crucial, as was the importance of collaboration and leveraging different insights from data generators and users to inform health policy.

Conclusions
Policy officers aware of KT are willing to utilize data visualization tools as they value them as an engaging and important mechanism to foster the use of GBD data in policy-making. To further facilitate policy officers’ efforts to effectively use GBD data in policy and practice, further research is required into how users perceive and interact with data visualization and other KT tools.
Multifaceted role of one-carbon metabolism on immunometabolic control and growth during pregnancy, lactation and the neonatal period in dairy cattle
Authors: Coleman Danielle N. , Alharthi Abdulrahman S. , Liang Yusheng , Lopes Matheus Gomes , Lopreiato Vincenzo , Vailati-Riboni Mario , Loor Juan J. 
Published: February 2021

AbstractDairy cattle undergo dramatic metabolic, endocrine, physiologic and immune changes during the peripartal period largely due to combined increases in energy requirements for fetal growth and development, milk production, and decreased dry matter intake. The negative nutrient balance that develops results in body fat mobilization, subsequently leading to triacylglycerol (TAG) accumulation in the liver along with reductions in liver function, immune dysfunction and a state of inflammation and oxidative stress. Mobilization of muscle and gluconeogenesis are also enhanced, while intake of vitamins and minerals is decreased, contributing to metabolic and immune dysfunction and oxidative stress. Enhancing post-ruminal supply of methyl donors is one approach that may improve immunometabolism and production synergistically in peripartal cows. At the cellular level, methyl donors (e.g. methionine, choline, betaine and folic acid) interact through one-carbon metabolism to modulate metabolism, immune responses and epigenetic events. By modulating those pathways, methyl donors may help increase the export of very low-density lipoproteins to reduce liver TAG and contribute to antioxidant synthesis to alleviate oxidative stress. Thus, altering one-carbon metabolism through methyl donor supplementation is a viable option to modulate immunometabolism during the peripartal period. This review explores available data on the regulation of one-carbon metabolism pathways in dairy cows in the context of enzyme regulation, cellular sensors and signaling mechanisms that might respond to increased dietary supply of specific methyl donors. Effects of methyl donors beyond the one-carbon metabolism pathways, including production performance, immune cell function, mechanistic target or rapamycin signaling, and fatty acid oxidation will also be highlighted. Furthermore, the effects of body condition and feeding system (total mixed ration vs. pasture) on one-carbon metabolism pathways are explored. Potential effects of methyl donor supply during the pepartum period on dairy calf growth and development also are discussed. Lastly, practical nutritional recommendations related to methyl donor metabolism during the peripartal period are presented. Nutritional management during the peripartal period is a fertile area of research, hence, underscoring the importance for developing a systems understanding of the potential immunometabolic role that dietary methyl donors play during this period to promote health and performance.
An acoustic sensor transmitter for in situ assessment of water quality and fish behaviour during chemical treatment of a parasite-infected river system: tag design and practical use
Authors: Alfredsen Knut Tore , Urke Henning Andre , Kristensen Torstein , Kvakland Marte , Gronningsater Aage , Hagen Anders Gjørwad , Alfredsen Jo Arve 
Published: February 2021

Abstract
Background
Behaviour of potential host fish during chemical treatment against the ectoparasite Gyrodactylus salaris is a vital factor in designing treatment strategies, evaluating risk factors and establishing insights into previously failed treatments. The effectiveness of any chemical treatment may be compromised if fish either are forced to, or seek out actively, areas of the river where the water quality is less affected by the chemicals. The aim of this study was to develop and apply an acoustic fish tag for fish localization with sensors for in situ measurement of water conductivity and temperature to investigate fish behaviour before, during and after an aluminium (Al) treatment. The sensor tag allowed discrimination between water qualities, and thereby quantification of exposure to treatment water.

Findings
Adult Atlantic salmon and anadromous brown trout from river Lærdalselva were tagged with external conductivity transmitters and followed daily by a network of passive receivers and by manual tracking 1 week ahead of treatment, during a 2-week aluminium (Al) treatment period and one week after an Al treatment. The results show no avoidance behaviour related to the Al treatment and most of the fish exhibited a behaviour during the treatment that did not differ significantly from the behaviour observed before or after the treatment. Data collected from the tags showed that the fish experienced increased conductivity during Al administration, suggesting successful exposure to treatment water. The tag gave verifiable environmental information and functioned well in the turbulent and acoustically demanding river environment, albeit with variable detection range.

Conclusions
The conductivity and temperature tag provided novel data on fish behaviour and exposure during the Al treatment period. Results show that fish exhibit normal behaviour during this period and no avoidance response can be detected in the collected data.
New ibuprofen derivatives with thiazolidine-4-one scaffold with improved pharmaco-toxicological profile
Authors: Vasincu Ioana-Mirela , Apotrosoaei Maria , Constantin Sandra , Butnaru Maria , Vereștiuc Liliana , Lupușoru Cătălina-Elena , Buron Frederic , Routier Sylvain , Lupașcu Dan , Taușer Roxana-Georgiana , Profire Lenuța 
Published: February 2021

Abstract
Background
Aryl-propionic acid derivatives with ibuprofen as representative drug are very important for therapy, being recommended especially for anti-inflammatory and analgesic effects. On other hand 1,3-thiazolidine-4-one scaffold is an important heterocycle, which is associated with different biological effects such as anti-inflammatory and analgesic, antioxidant, antiviral, antiproliferative, antimicrobial etc. The present study aimed to evaluated the toxicity degree and the anti-inflammatory and analgesic effects of new 1,3-thiazolidine-4-one derivatives of ibuprofen.

Methods
For evaluation the toxicity degree, cell viability assay using MTT method and acute toxicity assay on rats were applied. The carrageenan-induced paw-edema in rat was used for evaluation of the anti-inflammatory effect while for analgesic effect the tail-flick test, as thermal nociception in rats and the writhing assay, as visceral pain in mice, were used.

Results
The toxicological screening, in terms of cytotoxicity and toxicity degree on mice, revealed that the ibuprofen derivatives (4a-n) are non-cytotoxic at 2 μg/ml. In addition, ibuprofen derivatives reduced carrageenan-induced paw edema in rats, for most of them the maximum effect was recorded at 4 h after administration which means they have medium action latency, similar to that of ibuprofen. Moreover, for compound 4d the effect was higher than that of ibuprofen, even after 24 h of administration. The analgesic effect evaluation highlighted that 4 h showed increased pain inhibition in reference to ibuprofen in thermal (tail-flick assay) and visceral (writhing assay) nociception models.

Conclusions
The study revealed for ibuprofen derivatives, noted as 4 m, 4 k, 4e, 4d, a good anti-inflammatory and analgesic effect and also a safer profile compared with ibuprofen. These findings could suggest the promising potential use of them in the treatment of inflammatory pain conditions.
Emerging roles of C1Q tumor necrosis factor-related proteins in metabolic diseases
Authors: Ramanjaneya Manjunath , Jerobin Jayakumar , Bettahi Ilham , Siveen Kodappully Sivaraman , Abou-Samra Abdul-Badi 
Published: February 2021

AbstractObesity and insulin resistance are key elements of the metabolic syndrome, which includes type 2 diabetes (T2D), dyslipidemia, systemic inflammation, hypertension, elevated risk for cardiovascular diseases, non-alcoholic fatty liver disease (NAFLD) and polycystic ovary syndrome (PCOS). C1Q Tumor necrosis factor-related proteins (CTRPs) have recently emerged as important regulators of metabolism as a core component in the interrelationship between insulin resistance, adiposity and inflammation. To date 15 CTRP members have been identified and most of the CTRPs are dysregulated in obesity, T2D, coronary artery disease and NAFLD. Pharmacological intervention and lifestyle modification alter expression of CTRPs in circulation and in metabolically active tissues. CTRPs enhance metabolism mainly through activation of AMPK/AKT dependent pathways and possess insulin sensitizing properties. Thus dysregulated expression of CTRPs in metabolic disorders could contribute to the pathogenesis of the disease. For these reasons CTRPs appear to be promising targets for early detection, prevention and treatment of metabolic disorders. This review article aims at exploring the role of CTRPs in metabolic syndrome.
Domain-general cognitive motivation: evidence from economic decision-making
Authors: Crawford Jennifer L. , Eisenstein Sarah A. , Peelle Jonathan E. , Braver Todd S. 
Published: February 2021

AbstractStable individual differences in cognitive motivation (i.e., the tendency to engage in and enjoy effortful cognitive activities) have been documented with self-report measures, yet convergent support for a trait-level construct is still lacking. In the present study, we use an innovative decision-making paradigm (COG-ED) to quantify the costs of cognitive effort, a metric of cognitive motivation, across two distinct cognitive domains (working memory and speech comprehension). We hypothesize that cognitive motivation operates similarly within individuals, regardless of domain. Specifically, we test whether individual differences in effort costs are stable across domains, even after controlling for other potential sources of shared individual variation. Conversely, we evaluate whether the costs of cognitive effort across domains may be better explained in terms of other relevant cognitive and personality-related constructs, such as working memory capacity or reward sensitivity.
Application of Bacillus pumilus isolates for management of black rot disease in strawberry
Authors: Abd-El-Kareem Farid , Elshahawy Ibrahim E. , Abd-Elgawad Mahfouz M. M. 
Published: February 2021

Abstract
Background
Black root rot of strawberry plants caused by Rhizoctonia solani, Fusarium solani, and Pythium sp. is a serious disease in Egypt. Biocontrol agents have frequently proved to possess paramount and safe tools against many diseases. The impact of soil treatments with 3 Bacillus pumilus isolates on black root rot disease of strawberry plants caused by R. solani, F., and Pythium sp. under laboratory and field conditions was examined herein on the commonly used ‘Festival’ strawberry cultivar. To increase the bacterial adhesion and distribution on the roots, each seedling was dipped in bacterial cell suspension at 1 × 108 colony-forming units/ml of each separate bacterial isolate for 30 min then mixed with 5% Arabic gum.

Results
The tested B. pumilus isolates significantly reduced the growth area of these 3 fungi. The two bacterial isolates Nos. 2 and 3 reduced the growth area by more than 85.2, 83.6, and 89.0% for R. solani, F. solani, and Pythium sp., respectively. Likewise, the 3 bacterial isolates significantly (P ≤ 0.05) inhibited the disease under field conditions. Isolates Nos. 2 and 3 suppressed the disease incidence by 64.4 and 68.9% and disease severity by 65.3 and 67.3%, respectively. The fungicide Actamyl had effect similar to that of the 2 isolates. B. pumilus isolates significantly enhanced growth parameters and yields of strawberry plants; isolates Nos. 2 and 3 raised the yield by 66.7 and 73.3%, respectively.

Conclusions
Bacillus pumilus isolates could effectively manage the black rot disease in strawberry herein. Due to the significant impact of the root rot disease on strawberry yield, B. pumilus should be further tested to manage the disease on strawberry on large scale in Egypt.
Vaginal metastasis in solid tumours: our four cases and review of the literature
Authors: Korkmaz Mustafa , Eryılmaz Melek Karakurt , Kerimoğlu Ülkü , Karaağaç Mustafa , Demirkıran Aykut , Demir Emine Türen , Artaç Mehmet 
Published: February 2021

Abstract
Background
Vaginal metastasis should be kept in mind when evaluating the staging tests of all cancers, especially endometrial cancer.

Case presentation
We present four patients with vaginal recurrence who recently applied to our clinic. Three cases were of endometrial cancer and one case of rectal cancer. All patients presented with vaginal bleeding.

Conclusion
Standard treatment for vaginal metastasis has not yet been established. Therapeutic options for vaginal metastasis—separately or in combination—are surgical resection, radiotherapy, and chemotherapy.