bluuebunny/crossref_metadata_2025_split huggingface dataset:
Population Viability of Sea Turtles in the Context of Global Warming
Authors: Maurer Andrew S , Seminoff Jeffrey A , Layman Craig A , Stapleton Seth P , Godfrey Matthew H , Reiskind Martha O Burford 
Published: February 2021

Abstract
Sea turtles present a model for the potential impacts of climate change on imperiled species, with projected warming generating concern about their persistence. Various sea turtle life-history traits are affected by temperature; most strikingly, warmer egg incubation temperatures cause female-biased sex ratios and higher embryo mortality. Predictions of sea turtle resilience to climate change are often focused on how resulting male limitation or reduced offspring production may affect populations. In the present article, by reviewing research on sea turtles, we provide an overview of how temperature impacts on incubating eggs may cascade through life history to ultimately affect population viability. We explore how sex-specific patterns in survival and breeding periodicity determine the differences among offspring, adult, and operational sex ratios. We then discuss the implications of skewed sex ratios for male-limited reproduction, consider the negative correlation between sex ratio skew and genetic diversity, and examine consequences for adaptive potential. Our synthesis underscores the importance of considering the effects of climate throughout the life history of any species. Lethal effects (e.g., embryo mortality) are relatively direct impacts, but sublethal effects at immature life-history stages may not alter population growth rates until cohorts reach reproductive maturity. This leaves a lag during which some species transition through several stages subject to distinct biological circumstances and climate impacts. These perspectives will help managers conceptualize the drivers of emergent population dynamics and identify existing knowledge gaps under different scenarios of predicted environmental change.
A Highly Automated Mobile Laboratory for On-site Molecular Diagnostics in the COVID-19 Pandemic
Authors: Xing Wanli , Wang Jiadao , Zhao Chao , Wang Han , Bai Liang , Pan Liangbin , Li Hang , Wang Huili , Zhang Zhi , Lu Ying , Chen Xiang , Shan Sisi , Wang Dong , Pan Yifei , Weng Ding , Zhou Xinying , Huang Rudan , He Jianxing , Jin Ronghua , Li Weimin , Shang Hong , Zhong Nanshan , Cheng Jing 
Published: February 2021

Abstract

Background
Infectious disease outbreaks such as the COVID-19 (coronavirus disease 2019) pandemic call for rapid response and complete screening of the suspected community population to identify potential carriers of pathogens. Central laboratories rely on time-consuming sample collection methods that are rarely available in resource-limited settings.

Methods
We present a highly automated and fully integrated mobile laboratory for fast deployment in response to infectious disease outbreaks. The mobile laboratory was equipped with a 6-axis robot arm for automated oropharyngeal swab specimen collection; virus in the collected specimen was inactivated rapidly using an infrared heating module. Nucleic acid extraction and nested isothermal amplification were performed by a “sample in, answer out” laboratory-on-a-chip system, and the result was automatically reported by the onboard information platform. Each module was evaluated using pseudovirus or clinical samples.

Results
The mobile laboratory was stand-alone and self-sustaining and capable of on-site specimen collection, inactivation, analysis, and reporting. The automated sampling robot arm achieved sampling efficiency comparable to manual collection. The collected samples were inactivated in as short as 12 min with efficiency comparable to a water bath without damage to nucleic acid integrity. The limit of detection of the integrated microfluidic nucleic acid analyzer reached 150 copies/mL within 45 min. Clinical evaluation of the onboard microfluidic nucleic acid analyzer demonstrated good consistency with reverse transcription quantitative PCR with a κ coefficient of 0.979.

Conclusions
The mobile laboratory provides a promising solution for fast deployment of medical diagnostic resources at critical junctions of infectious disease outbreaks and facilitates local containment of SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2) transmission.
Lethal and Sublethal Effects of Eucalyptus camaldulensis and Mentha piperita Essential Oils on the Khapra Beetle (Coleoptera: Dermestidae) in Terms of Feeding Inhibition, Oviposition, and Seed Damage
Authors: Borzoui Ehsan , Khaghani Ramin , Nouri-Ganbalani Gadir 
Published: February 2021

Abstract
Trogoderma granarium Everts, the Khapra beetle, is a major pest of stored products, especially grains. In this study, fumigant toxicity and sublethal effects of Eucalyptus camaldulensis Dehnh. (Myrtaceae) and Mentha piperita L. (Lamiaceae) essential oils (EOs) were investigated against different growth stages of T. granarium. To assess the sublethal effects, insects were exposed to an LC20 or LC50 concentration of each essential oil, and the ability of these oils to deter feeding, oviposition, and damage to wheat seeds and overall mass were surveyed. At LC50 concentrations, M. piperita EO showed higher fumigant toxicity than E. camaldulensis EO against eggs, 2nd instar larvae, 4th instar larvae, and adults of T. granarium. Furthermore, the adults were more sensitive to the tested EOs than immatures. In free-choice tests, both larvae and adults showed a preference for control-treated wheat seeds than for seeds treated with an LC20 or LC50 concentration of EOs from E. camaldulensis or M. piperita. In a no-choice test, adult females exposed to EOs showed lower fecundity and fertility in comparison to control females not exposed to EOs. Treatment of wheat seeds with E. camaldulensis or M. piperita EOs resulted in a dose-specific reduction in the number of damaged seeds and seed weight loss when compared to control. According to our results, both tested EOs, especially EOs extracted from M. piperita, showed good potential for use in integrated pest management strategies against T. granarium.
Aortic valve-sparing root replacement with Tirone E. David’s reimplantation technique: single-centre 25-year experience
Authors: Beckmann Erik , Martens Andreas , Krüger Heike , Korte Wilhelm , Kaufeld Tim , Stettinger Alissa , Haverich Axel , Shrestha Malakh Lal 
Published: February 2021

Abstract

OBJECTIVES
Tirone E. David described aortic valve-sparing root reimplantion (AVSRR) almost 30 years ago. At our centre, we adopted this operation in 1993, and since then, we have performed &gt;700 operations over a time period of &gt;25 years. In this report, we present our single-centre experience.

METHODS
Between 1993 and 2019, a total of 732 patients underwent AVSRR at our centre. The mean age was 53 ± 15 and 522 (71%) were male. Marfan syndrome was present in 117 (16%) patients and bicuspid aortic valve in 81 (11%). The indication for surgery was aortic root aneurysm in 588 (80%) patients and acute aortic dissection in 144 (20%) patients.

RESULTS
Mini-sternotomy was performed in 74 (10%) patients. A straight tube graft (David I) was used in 677 (92%) and a Valsalva-graft in 55 (8%) patients. Cusp plasty was done in 83 (11%) patients. Concomitant cardiac procedures were performed in 438 (60%) patients. Overall in-hospital mortality was 3.8% (n = 29) and 1.9% (n = 11) in elective cases. Postoperative echocardiography was available for 671 patients and showed aortic insufficiency (AI) ≤I° in 647 (96%) patients. The mean follow-up time was 10 ± 6.7 years and comprised a total of 7307 patient-years. The 1-, 5-, 10- and 15-year survival rates were: 93%, 88%, 77% and 65%, respectively. The rates for freedom from valve-related reoperation at 1, 5, 10 and 15 years after initial surgery were 97%, 93%, 88% and 85%, respectively. Cox regression analysis identified age [odds ratio (OR) = 0.975, 95% confidence interval (CI) = 0.955–0.995, P = 0.016], hyperlipidaemia (OR = 1.980, 95% CI = 1.175–3.335, P = 0.010), preoperative level of left ventricular ejection function (OR = 1.749, 95% CI = 1.024–2.987, P = 0.041) and postoperative degree of AI (OR = 1.880, 95% CI = 1.532–2.308, P ≤ 0.001) as risk factors for the future AI or reoperation.

CONCLUSIONS
David procedure can be performed extremely safely, with low risks for perioperative morbidity and mortality, both in elective situations via minimally invasive access and in emergent settings for acute aortic type A dissection. Regarding long-term outcome, David‘s AVSRR seems to provide excellent clinical results and sustainable function of the aortic valve in the majority of patients almost 3 decades after its introduction.
Soil microbial communities influencing organic phosphorus mineralization in a coastal dune chronosequence in New Zealand
Authors: Gaiero Jonathan R , Tosi Micaela , Bent Elizabeth , Boitt Gustavo , Khosla Kamini , Turner Benjamin L , Richardson Alan E , Condron Leo M , Dunfield Kari E 
Published: February 2021

ABSTRACTThe Haast chronosequence in New Zealand is an ∼6500-year dune formation series, characterized by rapid podzol development, phosphorus (P) depletion and a decline in aboveground biomass. We examined bacterial and fungal community composition within mineral soil fractions using amplicon-based high-throughput sequencing (Illumina MiSeq). We targeted bacterial non-specific acid (class A, phoN/phoC) and alkaline (phoD) phosphomonoesterase genes and quantified specific genes and transcripts using real-time PCR. Soil bacterial diversity was greatest after 4000 years of ecosystem development and associated with an increased richness of phylotypes and a significant decline in previously dominant taxa (Firmicutes and Proteobacteria). Soil fungal communities transitioned from predominantly Basidiomycota to Ascomycota along the chronosequence and were most diverse in 290- to 392-year-old soils, coinciding with maximum tree basal area and organic P accumulation. The Bacteria:Fungi ratio decreased amid a competitive and interconnected soil community as determined by network analysis. Overall, soil microbial communities were associated with soil changes and declining P throughout pedogenesis and ecosystem succession. We identified an increased dependence on organic P mineralization, as found by the profiled acid phosphatase genes, soil acid phosphatase activity and function inference from predicted metagenomes (PICRUSt2).
AsaGEI2d: a new variant of a genomic island identified in a group of Aeromonas salmonicida subsp. salmonicida isolated from France, which bears the pAsa7 plasmid
Authors: Vincent Antony T , Intertaglia Laurent , Loyer Victor , Paquet Valérie E , Adouane Émilie , Martin Patrick , Bérard Céline , Lami Raphaël , Charette Steve J 
Published: February 2021

ABSTRACT
Genomic islands (Aeromonas salmonicida genomic islands, AsaGEIs) are found worldwide in many isolates of Aeromonas salmonicida subsp. salmonicida, a fish pathogen. To date, five variants of AsaGEI (1a, 1b, 2a, 2b and 2c) have been described. Here, we investigate a sixth AsaGEI, which was identified in France between 2016 and 2019 in 20 A. salmonicida subsp. salmonicida isolates recovered from sick salmon all at the same location. This new AsaGEI shares the same insertion site in the chromosome as the other AsaGEI2s as they all have a homologous integrase gene. This new AsaGEI was thus named AsaGEI2d, and has five unique genes compared to the other AsaGEIs. The isolates carrying AsaGEI2d also bear the plasmid pAsa7, which was initially found in an isolate from Switzerland. This plasmid provides resistance to chloramphenicol thanks to a cat gene. This study reveals more about the diversity of the AsaGEIs.
Disruption of barley immunity to powdery mildew by an in-frame Lys-Leu deletion in the essential protein SGT1
Authors: Chapman Antony V E , Hunt Matthew , Surana Priyanka , Velásquez-Zapata Valeria , Xu Weihui , Fuerst Greg , Wise Roger P 
Published: February 2021

AbstractBarley (Hordeum vulgare L.) Mla (Mildew resistance locus a) and its nucleotide-binding, leucine-rich-repeat receptor (NLR) orthologs protect many cereal crops from diseases caused by fungal pathogens. However, large segments of the Mla pathway and its mechanisms remain unknown. To further characterize the molecular interactions required for NLR-based immunity, we used fast-neutron mutagenesis to screen for plants compromised in MLA-mediated response to the powdery mildew fungus, Blumeria graminis f. sp. hordei. One variant, m11526, contained a novel mutation, designated rar3 (required for Mla6 resistance3), that abolishes race-specific resistance conditioned by the Mla6, Mla7, and Mla12 alleles, but does not compromise immunity mediated by Mla1, Mla9, Mla10, and Mla13. This is analogous to, but unique from, the differential requirement of Mla alleles for the co-chaperone Rar1 (required for Mla12 resistance1). We used bulked-segregant-exome capture and fine mapping to delineate the causal mutation to an in-frame Lys-Leu deletion within the SGS domain of SGT1 (Suppressor of G-two allele of Skp1, Sgt1ΔKL308–309), the structural region that interacts with MLA proteins. In nature, mutations to Sgt1 usually cause lethal phenotypes, but here we pinpoint a unique modification that delineates its requirement for some disease resistances, while unaffecting others as well as normal cell processes. Moreover, the data indicate that the requirement of SGT1 for resistance signaling by NLRs can be delimited to single sites on the protein. Further study could distinguish the regions by which pathogen effectors and host proteins interact with SGT1, facilitating precise editing of effector incompatible variants.
Childbirth, ‘Madness’, and Bodies in History
Authors: Carter Philippa 
Published: February 2021

Abstract
Looking at the casebooks kept by the early modern astrologer-physician Richard Napier, this article offers a close reading of cases in which he linked his patients’ ‘madness’ to their recent childbearing. Exploring this linkage, it engages with a longstanding historiographical debate about the relationship of culture, corporeality, and subjective embodiment. Napier’s ideas about childbirth-related mental ill health were profoundly gendered, and we cannot begin to understand them without studying the early modern gendering of planets, seasons, flesh, and blood. Contemporaries’ constructions of sex difference, in particular, underline the distance between their phenomenology of bodies and our own. Yet reading the case histories of these patients can give rise to impressions of familiarity, as well as strangeness. The article asks how historians should interpret the parallels between present-day understandings of childbirth-related health risks and those described in early modern England. It argues that we need to develop historical methodologies which allow room for both culture and the ‘extra-cultural’, even if we cannot separate out the two for scrutiny.
Performance characteristics of a heat pump using renewable transcritical CO2 refrigerant for heating water
Authors: Yunus Mohammed , Oreijah Mowffaq M 
Published: February 2021

ABSTRACT
Among non-toxic and inflammable working fluids, carbon dioxide (CO2) proving a possible choice of refrigerant is gaining full status because of its reducing capacity of global warming and ozone depletion. Heat pump (HP) equipment uses energy more rationally to heat water by reducing emissions and global warming caused by conventional refrigerants. HP capability demands an environmentally friendly refrigerant for their full utilization and high energy saving as well to attaining higher coefficient of performance (COP) without much design corrections. CO2 is checked for sustainability to eliminate the standardized refrigerants such as chlorofluorocarbons (CFC) and hydro-CFC. In this work, the performance of CO2 as an alternate refrigerant in HP for heating water at different pressure, flow rates, evaporator fan speed and preheating have been investigated. HP is accommodated with two condensers. The results showed that the increased mass flow rate had increased COP, overall heat transfer (HT) coefficient (U) and HT. However, logarithmic mean temperature difference was decreased for increasing evaporator’s fan speed and pressure. The outlet water temperature (TWO) of second condenser increased with decreasing water flow rate. Improved COP, HT and TWO of HP are observed from the experimental evaluation in case of preheating of water.
QuEChERS with Ultrasound-Assisted Extraction Combined with High-Performance Liquid Chromatography for the Determination of 16 Polycyclic Aromatic Hydrocarbons in Sediment
Authors: Wu Ling , He Qiurong , Zhang Jing , Li Yongxin , Yang Weiqing , Sun Chengjun 
Published: February 2021

Abstract

Background
Polycyclic aromatic hydrocarbons (PAHs) have attracted worldwide attention due to their carcinogenic, teratogenic, and mutagenic effects, environmental persistence, and bioaccumulation characteristics. Therefore, the sensitive, reliable, and rapid detection of PAHs in sediment is of great importance.

Objective
To develop a high-performance liquid chromatography (HPLC) with fluorescence and ultraviolet detection after Quick, Easy, Cheap, Effective, Rugged, and Safe (QuEChERS) treatment for simultaneous determination of 16 U.S. Environmental Protection Agency priority PAHs in sediment samples.

Method
The samples were ultrasonically extracted with acetone and then the supernatant was purified with a modified QuEChERS method. After centrifugation, the supernatant was injected into the HPLC system for analysis. The separation was accomplished on a ZORBAX Eclipse PAH column (150 × 4.6 mm, 3.5 μm) and the column temperature was set at 30 °C. The flow rate of the mobile phase consisting of water and acetonitrile in gradient elution mode was fixed at 0.9 mL/min. Detection was conducted on an ultraviolet detector and a fluorescence detector simultaneously. The qualitative analysis was based on retention time and the quantification was based on standard curves.

Results
Under the optimal conditions, this method showed good linearities in the range of 10–200 μg/L with correlation coefficients greater than 0.9993. The method had LODs ranging from 0.00108 to 0.314 ng/g. The mean recoveries ranged from 78.4 to 117% with intra-day and inter-day RSDs of 0.592–10.7% and 1.01–13.0%, respectively. The proposed method was successfully applied to the detection of 16 PAHs in sediment samples collected from the Funan River in Chengdu, China with total contents of 431–2143 ng/g·dw.

Conclusions
The established method is simple, rapid, environmentally friendly, and cost-effective. It can be applied to the analysis of 16 PAHs in sediment samples.

Highlights
A method of QuEChERS with ultrasound-assisted extraction combined with HPLC has been established for the analysis of 16 PAHs in sediment samples and the proposed method has been successfully applied to the analysis PAHs in real sediment samples.
Categorization of birth weight phenotypes for inclusion in genetic evaluations using a deep neural network
Authors: Ribeiro Andre , Golden Bruce L , Spangler Matthew L 
Published: February 2021

Abstract
Birth weight (BW) serves as a valuable indicator of the economically relevant trait of calving ease (CE), and erroneous data collection for BW could impact genetic evaluations for CE. The objective of the current study was to evaluate the use of deep neural networks (DNNs) for classifying contemporary groups (CGs) based on the method used to generate BW phenotypes. CGs (n = 120,000,000) ranging between 10 and 250 animals were simulated assuming 12 data collection and CG formation scenarios that could impact CG phenotypic variance, including weights recorded with a digital scale (REAL), hoof tape (TAPE), erroneous data collection (DIRTY), and those that were fabricated (FAB). The performance of eight activation functions (AFs; ReLu, Sigmoid, Exponential, ReLu6, Softmax, Softplus, Leaky ReLu, and Tanh) was evaluated. Four hidden layers were used with seven different scenarios relative to the number of neurons. Simulations were replicated 10 times. In general, accuracy (proportion of correct predictions) across AF and numbers of neurons were similar, with mean correlations ranging between 0.91 and 0.99. The AF ReLu, Sigmoid, Exponential, and ReLu6 had the greatest consistency (mean pair-wise correlation among replicates) with an average correlation of greater than 0.85. Independent of the number of neurons used, the sigmoid function produced the highest accuracy (0.99) and consistency (0.93). The model with the greatest accuracy and consistency was then applied to real BW data supplied by the American Hereford Association. In the real data, the lowest phenotypic variance was for FAB CG (2.65 kg2), REAL CG had the largest (15.84 kg2), and TAPE CG was intermediate (6.84 kg2). To investigate the potential impact of FAB data on routine genetic evaluations, CGs classified as FAB in 90% or more of the replicates were removed from the evaluation for CE, and the rank of resulting genetic predictions were compared with the case where records were not removed. The removal of FAB CG had a moderate impact on the prediction of CE expected progeny differences, primarily for animals with intermediate to high accuracy. The results suggest that a well-trained DNN can be effectively used to classify data based on quality metrics prior to the inclusion in routine genetic evaluation.
The Regeneration of Consumer Movement Solidarity
Authors: Chatzidakis Andreas , Maclaran Pauline , Varman Rohit 
Published: February 2021

Abstract
Consumer research has focused on the various resources and tactics that help movements achieve a range of institutional and marketplace changes. Yet, little attention has been paid to the persistence of movement solidarity, in particular its regeneration, despite a range of threats to it. Our research unpacks mechanisms that help consumer movement solidarity to overcome threats. Drawing on a 6-year ethnographic study of consumer movements in Exarcheia, a neighborhood in central Athens, Greece, we find that consumer movement solidarity persists despite a cataclysmic economic crisis that undermines their prevalent ideology and the emotional fatigue, that is, common in such movements. Three key mechanisms serve to overcome these threats: performative staging of collectivism, temporal tactics, and the emplacement of counter-sites. Overall, our study contributes to consumer research by illuminating how threats to solidarity are overcome by specific internal mechanisms that enable the regeneration of consumer movement solidarity.
Laryngeal perforation during Sistrunk procedure
Authors: Matos Leonor , Carvalho Lúcia , Alves Alexandre , Nora Mário 
Published: February 2021

Abstract
Thyroglossal duct cyst (TGDC) is the most common congenital neck anomaly in childhood, although it can be diagnosed at any age group. Ultrasound of the thyroid gland and the neck structures is an appropriate tool for diagnosis.
The authors aim to report a rare case of a 22-year-old male with a laryngeal perforation during a TGDC excision and raise awareness to this potentially life-threatening complication.
The Sistrunk procedure is the surgical treatment of choice with a low morbidity and recurrence rates.
Small bowel perforation after low-velocity trauma: a case report
Authors: Sinz S , Benigno L , Zadnikar M A , Biraima-Steinemann M E 
Published: February 2021

Abstract
We report the case of a 63-year-old patient with a low-velocity abdominal trauma and bowel perforation. The patient slipped on a wet floor and fell down the stairs. On admission, the patient complained about abdominal pain. A computed tomography scan showed traumatic hematoma of the jejunum in the left upper quadrant and a small amount of intra-abdominal air. Also rib fractures on the left side were diagnosed. We performed a diagnostic laparoscopy and found a bowel perforation, which was manually repaired.