bluuebunny/crossref_metadata_2025_split huggingface dataset:
Unveiling the neuromechanical mechanisms underlying the synergistic interactions in human sensorimotor system
Authors: Honarvar S. , Kim C. , Diaz-Mercado Y. , Koh K. , Kwon H. J. , Kiemel T. , Caminita M. , Hahn J. O. , Shim J. K. 
Published: January 2021

AbstractMotor synergies are neural organizations of a set of redundant motor effectors that interact with one another to compensate for each other’s error and ensure the stabilization of a performance variable. Recent studies have demonstrated that central nervous system synergistically coordinates its numerous motor effectors through Bayesian multi-sensory integration. Deficiency in sensory synergy weakens the synergistic interaction between the motor effectors. Here, we scrutinize the neuromechanical mechanism underlying this phenomenon through spectral analysis and modeling. We validate our model-generated results using experimental data reported in the literature collected from participants performing a finger force production task with and without tactile feedback (manipulated through injection of anesthetic in fingers). Spectral analysis reveals that the error compensation feature of synergies occurs only at low frequencies. Modeling suggests that the neurophysiological structures involving short-latency back-coupling loops similar to the well-known Renshaw cells explain the deterioration of synergy due to sensory deprivation.
DNA sequences performs as natural language processing by exploiting deep learning algorithm for the identification of N4-methylcytosine
Authors: Wahab Abdul , Tayara Hilal , Xuan Zhenyu , Chong Kil To 
Published: January 2021

AbstractN4-methylcytosine is a biochemical alteration of DNA that affects the genetic operations without modifying the DNA nucleotides such as gene expression, genomic imprinting, chromosome stability, and the development of the cell. In the proposed work, a computational model, 4mCNLP-Deep, used the word embedding approach as a vector formulation by exploiting deep learning based CNN algorithm to predict 4mC and non-4mC sites on theC.elegansgenome dataset. Diversity of ranges employed for the experimental such as corpus k-mer and k-fold cross-validation to obtain the prevailing capabilities. The 4mCNLP-Deep outperform from the state-of-the-art predictor by achieving the results in five evaluation metrics by following; Accuracy (ACC) as 0.9354, Mathew’s correlation coefficient (MCC) as 0.8608, Specificity (Sp) as 0.89.96, Sensitivity (Sn) as 0.9563, and Area under curve (AUC) as 0.9731 by using 3-mer corpus word2vec and 3-fold cross-validation and attained the increment of 1.1%, 0.6%, 0.58%, 0.77%, and 4.89%, respectively. At last, we developed the online webserverhttp://nsclbio.jbnu.ac.kr/tools/4mCNLP-Deep/, for the experimental researchers to get the results easily.
A bi-specific lectin from the mushroom Boletopsis grisea and its application in glycoanalytical workflows
Authors: Ganatra Mehul B. , Potapov Vladimir , Vainauskas Saulius , Francis Anthony Z. , McClung Colleen M. , Ruse Cristian I. , Ong Jennifer L. , Taron Christopher H. 
Published: January 2021

AbstractThe BLL lectin from the edible Japanese “Kurokawa” mushroom (Boletopsis leucomelaena) was previously reported to bind to N-glycans harboring terminal N-acetylglucosamine (GlcNAc) and to induce apoptosis in a leukemia cell line. However, its gene has not been reported. In this study, we used a transcriptomics-based workflow to identify a full-length transcript of a BLL functional ortholog (termed BGL) from Boletopsis grisea, a close North American relative of B. leucomelaena. The deduced amino acid sequence of BGL was an obvious member of fungal fruit body lectin family (Pfam PF07367), a highly conserved group of mushroom lectins with a preference for binding O-glycans harboring the Thomsen–Friedenreich antigen (TF-antigen; Galβ1,3GalNAc-α-) and having two ligand binding sites. Functional characterization of recombinant BGL using glycan microarray analysis and surface plasmon resonance confirmed its ability to bind both the TF-antigen and β-GlcNAc-terminated N-glycans. Structure-guided mutagenesis of BGL’s two ligand binding clefts showed that one site is responsible for binding TF-antigen structures associated with O-glycans, whereas the second site specifically recognizes N-glycans with terminal β-GlcNAc. Additionally, the two sites show no evidence of allosteric communication. Finally, mutant BGL proteins having single functional bindings site were used to enrich GlcNAc-capped N-glycans or mucin type O-glycopeptides from complex samples in glycomics and glycoproteomics analytical workflows.
Factors associated with left ventricular reverse remodelling after percutaneous coronary intervention in patients with left ventricular systolic dysfunction
Authors: Adachi Yusuke , Kiyosue Arihiro , Ando Jiro , Kawahara Takuya , Kodera Satoshi , Minatsuki Shun , Kikuchi Hironobu , Inaba Toshiro , Kiriyama Hiroyuki , Hirose Kazutoshi , Shinohara Hiroki , Saito Akihito , Fujiwara Takayuki , Hara Hironori , Ueda Kazutaka , Sakakura Kenichi , Hatano Masaru , Harada Mutsuo , Takimoto Eiki , Akazawa Hiroshi , Morita Hiroyuki , Momomura Shin-ichi , Fujita Hideo , Komuro Issei 
Published: January 2021

AbstractPercutaneous coronary intervention (PCI) is sometimes considered as an alternative therapeutic strategy to surgical revascularization in patients with coronary artery disease (CAD) and reduced left ventricular ejection fraction (LVEF). However, the types or conditions of patients that receive the clinical benefit of left ventricular reverse remodelling (LVRR) remain unknown. The purpose of this study was to investigate the determinants of LVRR following PCI in CAD patients with reduced LVEF. From 4394 consecutive patients who underwent PCI, a total of 286 patients with reduced LV systolic function (LVEF < 50% at initial left ventriculography) were included in the analysis. LVRR was defined as LV end-systolic volume reduction ≥ 15% and improvement of LVEF ≥ 10% at 6 months follow-up left ventriculography. Patients were divided into LVRR (n = 63) and non-LVRR (n = 223) groups. Multivariate logistic regression analysis revealed that unprotected left main coronary artery (LMCA) intervention was significantly associated with LVRR (P = 0.007, odds ratios [OR] 4.70, 95% confidence interval [CI] 1.54–14.38), while prior PCI (P = 0.001, OR 0.35, 95% CI 0.19–0.66), presence of in-stent restenosis (P = 0.016, OR 0.32, 95% CI 0.12–0.81), and presence of de-novo stenosis (P = 0.038, OR 0.36, 95% CI 0.14–0.95) were negatively associated with LVRR. These data suggest the potential prognostic benefit of unprotected LMCA intervention for LVRR and importance of angiographic follow-up in patients with CAD and LV systolic dysfunction.
Early age at menarche and metabolic cardiovascular risk factors: mediation by body composition in adulthood
Authors: Bubach Susana , Horta Bernardo Lessa , Gonçalves Helen , Assunção Maria Cecília Formoso 
Published: January 2021

AbstractEvidence suggests that early menarche increases cardiometabolic risk, and adiposity would be a possible mediator of this association. We assessed the association between age at menarche and metabolic cardiovascular risk factors and estimated the indirect effect of body composition in adulthood. In 1982, all hospital births in the city of Pelotas/Brazil, were identified and live births were examined and have been prospectively followed. At 30 years, information on age at menarche and metabolic cardiovascular risk factors was available for 1680 women. Mediation analysis was performed using G-computation to estimate the direct effect of age at menarche and the indirect effect of body composition. The prevalence of age at menarche < 12 years was 24.5% and was associated with higher mean diastolic blood pressure [β: 1.98; 95% CI: 0.56, 3.40], total cholesterol (β: 8.28; 95% CI: 2.67, 13.88), LDL-cholesterol (β: 6.53; 95% CI: 2.00, 11.07), triglycerides (β: 0.11; 95% CI: 0.03, 0.19). For diastolic blood pressure, total cholesterol, LDL-cholesterol, triglycerides, body composition assessed by fat mass index captured from 43.8 to 98.9% of the effect of early menarche, except to systolic blood pressure, HDL-cholesterol, C-reactive-protein. Suggesting that the effect of menarche age < 12 years on some metabolic cardiovascular risk factors is mediated partially by body composition in adulthood.
Bioavailable fluoride in calcium-containing dentifrices
Authors: Shen Peiyan , Fernando James R. , Yuan Yi , Walker Glenn D. , Reynolds Coralie , Reynolds Eric C. 
Published: January 2021

AbstractCalcium added to dentifrices can complex with fluoride ions to reduce intra-oral bioavailability and therefore efficacy in preventing dental caries. Six commercially available dentifrices containing different types of calcium and fluoride were analyzed for total and bioavailable fluoride levels by adding 10 g of dentifrice to 30 mL of distilled deionized water and mixing vigorously for 1 min to simulate toothbrushing. One milliliter of the dentifrice/water slurry was immediately centrifuged and the supernatant removed for bioavailable fluoride analysis and the mixed slurry prior to centrifugation used for total fluoride analysis using a modified microdiffusion method. The concentration of fluoride was determined using a fluoride ion-selective electrode calibrated with internal fluoride standards. All the dentifrices had similar total fluoride concentrations to those indicated on their labels (94% to 105%). However, only one dentifrice that contained calcium in the form of casein phosphopeptide amorphous calcium phosphate (CPP-ACP) had almost 100% (97%) of fluoride in bioavailable form. The other dentifrices contained calcium carbonate and they exhibited significantly (p < 0.001) lower bioavailable fluoride levels (27% to 61%), through the generation of poorly soluble fluoride phases. The saliva biomimetic CPP, as CPP-ACP, in a dentifrice stabilised calcium and fluoride ions to maintain fluoride’s bioavailability.
Nuclear IL-33 restrains the early conversion of fibroblasts to an extracellular matrix-secreting phenotype
Authors: Gatti Francesca , Mia Sobuj , Hammarström Clara , Frerker Nadine , Fosby Bjarte , Wang Junbai , Pietka Wojciech , Sundnes Olav , Hol Johanna , Kasprzycka Monika , Haraldsen Guttorm 
Published: January 2021

AbstractInterleukin (IL)-33 is a cytokine that appears to mediate fibrosis by signaling via its receptor ST2 (IL-33R/IL1RL1). It is also, however, a protein that after synthesis is sorted to the cell nucleus, where it appears to affect chromatin folding. Here we describe a novel role for nuclear IL-33 in regulating the fibroblast phenotype in murine kidney fibrosis driven by unilateral ureteral obstruction. Transcriptional profiling of IL-33-deficient kidneys 24 h after ligation revealed enhanced expression of fibrogenic genes and enrichment of gene sets involved in extracellular matrix formation and remodeling. These changes relied on intracellular effects of IL-33, because they were not reproduced by treatment with a neutralizing antibody to IL-33 that prevents IL-33R/ST2L receptor signaling nor were they observed in IL-33R/ST2-deficient kidneys. To further explore the intracellular function of IL-33, we established transcription profiles of human fibroblasts, observing that knockdown of IL-33 skewed the transcription profile from an inflammatory towards a myofibroblast phenotype, reflected in higher levels of COL3A1, COL5A1 and transgelin protein, as well as lower expression levels of IL6, CXCL8, CLL7 and CCL8. In conclusion, our findings suggest that nuclear IL-33 in fibroblasts dampens the initial profibrotic response until persistent stimuli, as enforced by UUO, can override this protective mechanism.
Effects of different vibration frequencies on muscle strength, bone turnover and walking endurance in chronic stroke
Authors: Yang Zhenhui , Miller Tiev , Xiang Zou , Pang Marco Y. C. 
Published: January 2021

AbstractThis randomized controlled trial aimed to evaluate the effects of different whole body vibration (WBV) frequencies on concentric and eccentric leg muscle strength, bone turnover and walking endurance after stroke. The study involved eighty-four individuals with chronic stroke (mean age = 59.7 years, SD = 6.5) with mild to moderate motor impairment (Fugl-Meyer Assessment lower limb motor score: mean = 24.0, SD = 3.5) randomly assigned to either a 20 Hz or 30 Hz WBV intervention program. Both programs involved 3 training sessions per week for 8 weeks. Isokinetic knee concentric and eccentric extension strength, serum level of cross-linked N-telopeptides of type I collagen (NTx), and walking endurance (6-min walk test; 6MWT) were assessed at baseline and post-intervention. An intention-to-treat analysis revealed a significant time effect for all muscle strength outcomes and NTx, but not for 6MWT. The time-by-group interaction was only significant for the paretic eccentric knee extensor work, with a medium effect size (0.44; 95% CI: 0.01, 0.87). Both WBV protocols were effective in improving leg muscle strength and reducing bone resorption. Comparatively greater improvement in paretic eccentric leg strength was observed for the 30 Hz protocol.
Geological and Pleistocene glaciations explain the demography and disjunct distribution of red panda (A. fulgens) in eastern Himalayas
Authors: Dalui Supriyo , Singh Sujeet Kumar , Joshi Bheem Dutt , Ghosh Avijit , Basu Shambadeb , Khatri Hiren , Sharma Lalit Kumar , Chandra Kailash , Thakur Mukesh 
Published: January 2021

AbstractPleistocene glaciations facilitated climatic oscillations that caused for enormous heterogeneity in landscapes, and consequently affected demography and distribution patterns of the mountain endemic species. In this context, we investigated demographic history and population genetic structure of red panda, distributed along the geographical proximity in the southern edge of the Qinghai-Tibetan Plateau. Bayesian based phylogeny demonstrated that red panda diverged about 0.30 million years ago (CI 0.23–0.39) into two phylogenetic (sub) species, that correspond to the middle-late Pleistocene transition. The observed intraspecific clades with respect to Himalayan and Chinese red panda indicated restricted gene flow resulting from the Pleistocene glaciations in the eastern and southern Tibetan Plateau. We found Himalayan red panda population at least in KL-India declined abruptly in last 5–10 thousand years after being under demographic equilibrium. We suggest revisiting the ongoing conservation activities through cross border collaboration by developing multi-nationals, and multi-lateral species-oriented conservation action plans to support the red panda populations in transboundary landscapes.
The negative elongation factor NELF promotes induced transcriptional response of Drosophila ecdysone-dependent genes
Authors: Mazina Marina Yu. , Kovalenko Elena V. , Vorobyeva Nadezhda E. 
Published: January 2021

AbstractFor many years it was believed that promoter-proximal RNA-polymerase II (Pol II) pausing manages the transcription of genes inDrosophiladevelopment by controlling spatiotemporal properties of their activation and repression. But the exact proteins that cooperate to stall Pol II in promoter-proximal regions of developmental genes are still largely unknown. The current work describes the molecular mechanism employed by the Negative ELongation Factor (NELF) to control the Pol II pause at genes whose transcription is induced by 20-hydroxyecdysone (20E). According to our data, the NELF complex is recruited to the promoters and enhancers of 20E-dependent genes. Its presence at the regulatory sites of 20E-dependent genes correlates with observed interaction between the NELF-A subunit and the ecdysone receptor (EcR). The complete NELF complex is formed at the 20E-dependent promoters and participates in both their induced transcriptional response and maintenance of the uninduced state to keep them ready for the forthcoming transcription. NELF depletion causes a significant decrease in transcription induced by 20E, which is associated with the disruption of Pol II elongation complexes. A considerable reduction in the promoter-bound level of the Spt5 subunit of transcription elongation factor DSIF was observed at the 20E-dependent genes upon NELF depletion. We presume that an important function of NELF is to participate in stabilizing the Pol II-DSIF complex, resulting in a significant impact on transcription of its target genes. In order to directly link NELF to regulation of 20E-dependent genes in development, we show the presence of NELF at the promoters of 20E-dependent genes during their active transcription in both embryogenesis and metamorphosis. We also demonstrate that 20E-dependent promoters, while temporarily inactive at the larval stage, preserve a Pol II paused state and bind NELF complex.
Biases in ecological research: attitudes of scientists and ways of control
Authors: Zvereva Elena L. , Kozlov Mikhail V. 
Published: January 2021

AbstractThe properties of the human mind affect the quality of scientific knowledge through the insertion of unconscious biases during the research process. These biases frequently cause overestimation of the effects under study, thereby violating the reproducibility of the research and potentially leading to incorrect conclusions in subsequent research syntheses. We explored the level of knowledge about biases and attitudes to this problem by analysing 308 responses of ecology scientists to a specifically developed survey. We show that knowledge about biases and attitude towards biases depend on the scientist’s career stage, gender and affiliation country. Early career scientists are more concerned about biases, know more about measures to avoid biases, and twice more frequently have learned about biases from their university courses when compared with senior scientists. The respondents believe that their own studies are less prone to biases than are studies by other scientists, which hampers the control of biases in one’s own research. We conclude that education about biases is necessary, but not yet sufficient, to avoid biases because the unconscious origin of biases necessitates external intervention to combat them. Obligatory reporting of measures taken against biases in all relevant manuscripts will likely enhance the reproducibility of scientific results.
Longitudinal changes in retinal microvasculature after panretinal photocoagulation in diabetic retinopathy using swept-source OCT angiography
Authors: Kim Kiyoung , Kim Eung Suk , Yu Seung-Young 
Published: January 2021

AbstractThis study evaluated quantitative changes in microvascular parameters after panretinal photocoagulation (PRP) in diabetic retinopathy (DR), using swept-source OCT Angiography (SS-OCTA). A total of 27 treatment-naïve eyes were subjected to PRP and followed-up for > 12 months after the procedure. Foveal avascular zone (FAZ) area, macular perfusion density (PD), and vessel length density (VLD) were calculated on a 3 × 3 mm en face OCTA image and nonperfusion area (NPA) was obtained on a 12 × 12 mm en face OCTA image. One month after PRP, PD and VLD of superficial and deep capillary plexus decreased and subsequently, increased progressively across the next 12 months, with statistically significant differences (P = 0.015 and 0.02). Continuous decreasing trends in total NPA values was observed across 12 months after PRP (P = 0.125). A difference in PD of the superficial capillary plexus between baseline and 6 months post PRP, was significantly associated with the progression of DR, 12 months after PRP (OR 0.528; P = 0.025). We found significant longitudinal retinal microvascular changes after PRP in DR. Overall macular perfusion status was impaired and progressively recovered across the next 12 months, compared to the baseline. Additionally, the early treatment responses in PD can predict the long-term outcomes of PDR after PRP.
Spatial variations in the warming trend and the transition to more severe weather in midlatitudes
Authors: Estrada Francisco , Kim Dukpa , Perron Pierre 
Published: January 2021

AbstractDue to various feedback processes called Arctic amplification, the high-latitudes’ response to increases in radiative forcing is much larger than elsewhere in the world, with a warming more than twice the global average. Since the 1990’s, this rapid warming of the Arctic was accompanied by no-warming or cooling over midlatitudes in the Northern Hemisphere in winter (the hiatus). The decrease in the thermal contrast between Arctic and midlatitudes has been connected to extreme weather events in midlatitudes via, e.g., shifts in the jet stream towards the equator and increases in the probability of high-latitude atmospheric blocking. Here we present an observational attribution study showing the spatial structure of the response to changes in radiative forcing. The results also connect the hiatus with diminished contrast between temperatures over regions in the Arctic and midlatitudes. Recent changes in these regional warming trends are linked to international actions such as the Montreal Protocol, and illustrate how changes in radiative forcing can trigger unexpected responses from the climate system. The lesson for climate policy is that human intervention with the climate is already large enough that even if stabilization was attained, impacts from an adjusting climate are to be expected.
Slow deformation event between large intraslab earthquakes at the Tonga Trench
Authors: Mitsui Yuta , Muramatsu Hinako , Tanaka Yusaku 
Published: January 2021

AbstractSlow deformations associated with a subducting slab can affect quasi-static displacements and seismicity over a wide range of depths. Here, we analyse the seismotectonic activities in the Tonga subduction zone, which is the world’s most active area with regard to deep earthquakes. In our study, we combine data from global navigation satellite systems with an earthquake catalogue. We focus on the deep earthquakes that are below 400 km at the lower part of the Wadati–Benioff zone. We find that trenchward transient displacements and quiescence of deep earthquakes, in terms of background seismicity, were bounded in time by large intraslab earthquakes in 2009 and 2013. This “slow deformation event” between 2009 and 2013 may have been triggered by a distant and shallow M8.1 earthquake, which implies a slow slip event at the plate interface or a temporal acceleration of the subduction of the Pacific Plate. These findings provide new insights into the relationship between shallow and deep earthquakes in the subduction zone.
Passivation of InP solar cells using large area hexagonal-BN layers
Authors: Raj Vidur , Chugh Dipankar , Black Lachlan E. , Shehata M. M. , Li Li , Kremer Felipe , Macdonald Daniel H. , Tan Hark Hoe , Jagadish Chennupati 
Published: January 2021

AbstractSurface passivation is crucial for many high-performance solid-state devices, especially solar cells. It has been proposed that 2D hexagonal boron nitride (hBN) films can provide near-ideal passivation due to their wide bandgap, lack of dangling bonds, high dielectric constant, and easy transferability to a range of substrates without disturbing their bulk properties. However, so far, the passivation of hBN has been studied for small areas, mainly because of its small sizes. Here, we report the passivation characteristics of wafer-scale, few monolayers thick, hBN grown by metalorganic chemical vapor deposition. Using a recently reported ITO/i-InP/p+-InP solar cell structure, we show a significant improvement in solar cell performance utilizing a few monolayers of hBN as the passivation layer. Interface defect density (at the hBN/i-InP) calculated using C–V measurement was 2 × 1012 eV−1cm−2 and was found comparable to several previously reported passivation layers. Thus, hBN may, in the future, be a possible candidate to achieve high-quality passivation. hBN-based passivation layers can mainly be useful in cases where the growth of lattice-matched passivation layers is complicated, as in the case of thin-film vapor–liquid–solid and close-spaced vapor transport-based III–V semiconductor growth techniques.