bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification of Key Iron Metabolism-Related Long Non-Coding RNAs in Hepatocellular Carcinoma Based on Bioinformatics Analysis
Authors: chen yu 
Published: August 2021

Abstract
Background： The present study explored the regulatory mechanisms and functional roles of iron metabolism-related long non-coding RNAs (lncRNAs) in hepatocellular carcinoma (HCC) and their potential impact on prognosis of HCC patients. Methods：RNA-seq data and clinical information of HCC samples and normal samples were downloaded from The Cancer Genome Atlas (TCGA) database and International Cancer Genome Consortium (ICGC) portal. Iron metabolism-related genes were downloaded from Reactome database and AmiGo2 database. Differential expression and correlation analysis were performed to identify iron metabolism-related differentially expressed lncRNAs (DElncRNAs). Moreover, Kaplan-Meier (KM) survival and receiver operating characteristic (ROC) analysis were used to screen the possible prognostic and diagnostic biomarkers of HCC. Results: A total of 20 differentially expressed and iron metabolism-related genes (DEIMRG) were identified by overlapping 3746 differentially expressed genes (DEGs) and 86 IMRGs. Next, ARHGAP11B, LINC00205, LINC00261 and SNHG12 were screened through Univariate Cox regression. Kaplan-Meier survival curves indicated that ARHGAP11B, LINC00205, LINC00261 and SNHG12 were related to overall survival (OS) in HCC patient in TCGA database. ARHGAP11B, LINC00205 and LINC00261 were finally identified as prognostic DEIMRGs related with OS of HCC patients after validate the survival results in ICGC portal. ARHGAP11B, LINC00205 and LINC00261 all achieved an AUC value of >0.80 in ROC curve analysis. Furthermore, LINC00205 was identified as independently prognostic factor by multivariate Cox analysis combined with clinicopathological factors. Moreover, a ceRNA network including 25 DEmRNAs, 15 DEmiRNA and 3 DElncRNAs was successfully constructed, based on prognostic DElncRNAs and key target miRNAs and mRNAs of them predicted by starBase database and miRwalk. The PPI network illustrated that CDC25A, CHEK1, CCNE2 and ANLN proteins interact more with other proteins. Conclusions: In the present study, we identified iron metabolism related LINC00205 as a prognostic and diagnostic biomarker and constructed a metabolism-related ceRNA network, which may contribute to the treatment of HCC.
Expanding the Chemical Space of Aryloxy-naphthoquinones as Potential Anti-chagasic Agents: Synthesis and Trypanosomicidal Activity
Authors: Becerra Nohemí A. , Espinosa-Bustos Christian , Vázquez Karina , Rivera Gildardo , Paulino Margot , Cantero Jorge , Nogueda Benjamín , Chacón-Vargas Fabiola , Castillo-Velazquez Uziel , Rodríguez Ana F. Elizondo , Toledo Sofía , Moreno-Rodríguez Adriana , Aranda Mario , salas cristian osvaldo 
Published: August 2021

Abstract
In continuation our effort to research the chemical space of aryloxy-naphthoquinones as potential anti-Chagas agents, we synthesized nine derivatives and these compounds were evaluated in vitro against the epimastigote and trypomastigote forms of Mexican strains of Trypanosoma cruzi (T. cruzi). Most of these derivatives are highly active against epimastigote forms (IC50 < 1.0 µM) compared to the reference drug benznidazole (Bzn). Then these were evaluated on trypomastigotes, which is showing better potency results than Bzn for compounds 3b and 3g. In addition, the cytotoxicity of these compounds was determined on the murine macrophage cell line J774. 3b and 3i were the most selective compounds against NINOA trypomastigote and INC-5 epimastigote forms, respectively. Further these compounds also have good oral bioavailability according to theoretical predictions. Finally, we were able to determine optimal substitution patterns using pharmacophoric models. All these results are provided very useful structural information to continue our designing of naphthoquinone derivatives against T. cruzi.
Platelet Toll-like Receptor 4-related Innate Immunity Potentially Participates in Transfusion Reactions Independent of ABO Compatibility: An ex Vivo Study
Authors: Tsai Chien-Sung , Hu Mei-Hua , Hsu Yung-Chi , Huang Go-Shine 
Published: August 2021

Abstract
Purpose: The role of platelet TLR4 in transfusion reactions remains unclear. This study analyzed platelet TLR4, certain DAMPs, and the effect of ABO compatibility on TLR4 expression after a simulated transfusion ex vivo.Methods: Donor red blood cells were harvested from a blood bank. Recipient blood from patients undergoing cardiac surgery was processed to generate a washed platelet suspension. Donor blood was added to the washed platelets at 1%, 5%, or 10% (v/v). Blood mixing experiments were performed using four groups: 0.9% saline control group (n = 31); M, matched blood type mixing (n = 20); S, uncross-matched ABO type-specific mixing (n = 20); and I, ABO incompatible blood mixing (n = 20). Platelet TLR4 expression was determined after blood mixing. Levels of TLR4-binding DAMPs (HMGB1, S100A8, S100A9, and SAA) and that of LPS-binding protein and endpoint proteins (TNF-α, IL-1β, and IL-6) in the TLR4 signaling pathway were evaluated.Results: The 1%, 5%, and 10% blood mixtures significantly increased TLR4 expression in three groups (M, S, and I; all P < 0.001) in a concentration-dependent manner. TLR4 expression did not significantly differ among the three groups (P = 0.148). HMGB1, S100A8, and S100A9 showed elevated levels in response to blood mixing; SAA, LPS-binding protein, TNF-α, IL-1β, and IL-6 did not. Conclusion: Blood mixing may elicit innate immune responses by upregulating platelet TLR4 and DAMPs unassociated with ABO compatibility, suggesting that innate immunity through TLR4-mediated signaling may induce transfusion reactions. The trial was retrospectively registered at Chinese Clinical Trial Registry (ChiCTR2100045606) with date of registration on 19 April 2021.
Entropy Analysis and Thermal Characteristics of Reiner Philippoff Hybrid Nanofluidic Flow Via a Parabolic Trough of Solar Aircraft Wings: Keller Box Method
Authors: Sajid Tanveer , Jamshed Wasim , Shahzad Faisal , Eid Mohamed R. , Akgül Esra Karataş , Nisar Kottakkaran Sooppy 
Published: August 2021

Abstract
Solar energy is about the study of solar radiations and a method to enhance the efficacy of solar aircrafts with the utilization of solar radiations and nanotechnology. Solar radiations has been considered a heat source. The heat transmission performance of the wings is scrutinized for the situation of various effects like thermal radiations, heat generation, variant thermal conductance, thermal conductivity, and viscidness dissipative flow. Entropy generation analysis has been carried out in the status of Reiner Philippoff nanofluid (RPNF). The performance of the solar aircraft wings (SACW) improves in relations of thermal transmission for the status of amplification in thermal radiation, heat generation, viscidness dissipative flowing, and thermal conductivity parameters.
Application of a New Anatomic Hook-rod-pedicle Screw System in Young Patients With Lumbar Spondylolysis: a Pilot Study
Authors: Li Duanming , Jiang Wei , Peng Baogan 
Published: August 2021

Abstract
Background: Pedicle screw-laminar hook system has strong fixation and is conducive to bone graft fusion for lumbar spondylolysis. However, the current pedicle screw-laminar hook fixation system is not specifically designed for lumbar spondylolysis. Methods: We designed a new anatomic hook-rod-pedicle screw system for young patients with lumbar spondylolysis. The isthmus and the corresponding pedicle screw entry point were exposed through intermuscular approach. Autogenous iliac bone graft was obtained to bridge the isthmus defect, and then anatomic hook-rod-pedicle screw system was used to fix the isthmus in 15 young patients. Results: At 24 months follow-up, the Visual analogue scale (VAS) score of low back pain decreased from 6.73 ± 0.88 to 0.73 ± 0.59, and the Oswestry disability index (ODI) score decreased from 58.20 ± 8.99 to 7.87 ± 4.97. Computed tomography (CT) showed bilateral isthmic bone healing in 14 cases and unilateral isthmic bone healing in 1 case. Magnetic resonance imaging (MRI) showed that the lumbar disc signal of diseased segment and adjacent segments had no change compared with that before surgery. The pain VAS score of donor site was 0.20±0.41 at the last follow-up. According to Macnab score, the excellent and good rate was 100%.Conclusions: The application of this new anatomical hook-rod-pedicle screw system to treat young patients with lumbar spondylolysis has the advantages of less trauma, simple operation and satisfactory clinical effects.
Begomovirus Caboniensis: a New Bipartite Begomovirus Isolated From Cnidoscolus Urens in Brazil
Authors: Chaves Lívia Francyne Gomes , Ferro Mayra M.M. , de Lima Mayara O. , Assunção Iraildes P. , Lima Gaus S.A. , Silva Sarah J.C. da 
Published: August 2021

Abstract
A novel bipartite begomovirus infecting Cnidoscolus urens (Euphorbiaceae) from Pernambuco State, Brazil has been characterized. The complete DNA-A (2657 to 2692 nt) and DNA-B (2622 nt) components of the viral isolates showed a typical genome organization of New World bipartite begomoviruses. DNA-A of the isolates had the highest percentage of nucleotide identity (88.6–88.9%) with the Cnidoscolus mosaic leaf deformation virus (NC_038982). Based on the current classification criteria for the genus Begomovirus, a new member infecting C. urens was reported, and the name Begomovirus caboniensis was proposed for these viruses, adopting the standardized binomial system.
Spreading of Disturbances in Realistic Models of Transmission Grids: Dependence on Topology, Inertia and Heterogeneity
Authors: Nnoli Kosisochukwu P. , Kettemann Stefan 
Published: August 2021

Abstract
The energy transition towards more renewable energy resources (RER) profoundly affects the frequency dynamics and stability of electrical power networks. Here, we investigate systematically the effect of reduced grid inertia, due to an increase in the magnitude of RER, its heterogeneous distribution and the grid topology on the propagation of disturbances in realistic power grid models. These studies are conducted with the DigSILENT PowerFactory software. By changing the power generation at one central bus in each grid at a specific time, we record the resulting frequency transients at all buses. Plotting the time of arrival (ToA) of the disturbance at each bus versus the distance from the disturbance, we analyse its propagation throughout the grid. While the ToAs are found to be distributed, we confirm a tendency that the ToA increases with geodesic distance linearly. Thereby, we can measure an average velocity of propagation by fitting the data with a ballistic equation. This velocity is found to decay with increasing inertia. Characterising each grid by its meshedness coefficient, we find that the distribution of the ToAs depends in more meshed grids less strongly on the grid inertia. In order to take into account the inhomogeneous distribution of inertia, we introduce an effective distance reff, which is weighted with a factor which strongly depends on local inertia. We find that this effective distance is more strongly correlated with the ToAs, for all grids. This is confirmed quantitatively by obtaining a larger Pearson correlation coefficient between ToA and reff than with r. Remarkably, a ballistic equation for the ToA with a velocity, as derived from the swing equation, provides a strict lower bound for all effective distances reff in all power grids. thereby yielding a reliable estimate for the smallest time a disturbance needs to propagate that distance as function of system parameters, in particular inertia. We thereby conclude that in the analysis of contingencies of power grids it may be advisable that system designers and operators use the effective distance reff, taking into account inhomogeneous distribution of inertia as introduced in Eq. (11), to locate a disturbance. Moreover, our results provide evidence for the importance of the network topology as quantified by the meshedness coefficient β.
High Intraoperative Pulse Pressure Is A Risk Factor For A Postoperative Organ Injury In A Cohort of Abdominal Surgery Patients: An Exploratory Study
Authors: Putowski Zbigniew , Czajka Szymon , Krzych Łukasz J. 
Published: August 2021

Abstract
Background: Both intraoperative hypotension and hypertension have been reported to increase the occurrence of either acute kidney injury (AKI), myocardial infraction (MI) or stroke. However, intraoperative pulse pressure’s (PP) impact on the latter complications remains relatively unknown.Methods: This is a cohort study in which patients who underwent abdominal surgery between 1 October 2018 and 15 July 2019 in university hospital in Katowice were included in the analysis. Pre- and intraoperative data, including blood pressure measurements, were acquired via medical charts. Several PP thresholds were applied: >50, >55, >60, >65, >70, >75, >80, >85, >90 mmHg. Additionally, by analysing the maximal PP during procedures, a cut-off point for the occurrence of outcomes was estimated. Postoperative complications were defined as occurrence of either AKI, MI or stroke. Univariable and multivariable analyses were performed to assess PP’s relationship with hypoperfusive organ injury.Results: 508 patients were included in the analysis. Hypoperfusion was present in 38 (7.5%) cases. ROC curve analysis estimated a cut-off point of > 74 mmHg of maximal PP to be associated with the outcomes. PP values above 65 mmHg onward were included in the multivariable statistical models. A model in which PP > 90 mmHg (OR=4.21; 95%CI 1.73-10.24; p=0.0015) was included, had the best predicting value in predicting hypoperfusive injury. Apart of PP, intraoperative hypotension, presence of chronic arterial hypertension and procedure duration were independently associated with postoperative complications. Conclusions: High intraoperative pulse pressure may be associated with the occurrence of hypoperfusion-related organ injury. However, the effect of high pulse pressure should be confirmed in other non-cardiac populations to prove generalizability of our results.
Habitat disturbance Linked with Host Microbiome Dispersion and Bd Dynamics in Temperate Amphibians
Authors: Neely Wesley James , Greenspan Sasha E , Stahl Leigha M , Heraghty Sam D , Marshall Vanessa M , Atkinson Carla L , Becker Carlos Guilherme 
Published: August 2021

Abstract
Anthropogenic habitat disturbances can dramatically alter ecological community interactions, including host-pathogen dynamics. Recent work has highlighted the potential for habitat disturbances to alter host-associated microbial communities, but the associations between anthropogenic disturbance, host microbiomes, and pathogens are unresolved. Amphibian skin microbial communities are particularly responsive to factors like temperature, physiochemistry, pathogen infection, and environmental microbial reservoirs. Through a field survey on wild populations of Acris crepitans (Hylidae) and Lithobates catesbeianus (Ranidae), we assessed effects of habitat disturbance on environmental bacterial resevoirs, Batrachochytrium dendrobatidis (Bd) infection, and skin microbiome composition. We found higher measures of microbiome dispersion (a measure of community stability) in A. crepitans from more disturbed ponds, supporting the hypothesis that disturbance increases stochasticity in biological communties. We also found that habitat disturbance limited microbiome similarity between locations for both species, suggesting less bacterial exchange in more disturbed areas. Higher disturbance was associated with lower Bd prevalence for A. crepitans, which could signify suboptimal microclimates for Bd in disturbed habitats. In this system we use microbiome dispersion as a metric of population health. Combined, our findings show that reduced microbiome stability stemming from habitat disturbance could compromise population health, even in the absence of pathogenic infection.
Comparison Study of Elastic, Physical and Structural Properties for Strontium Oxide and Manganese Oxide in Borotellurite Glasses for High Strength Glass Application
Authors: NIDZAM NAZIRUL NAZRIN SHAHROL , Kamari Halimah Mohamed Mohamed , Sukari Muhammad Syaamil Mohd , Alauddin Fatin Azira Mohamad , Laoding Hasnimulyati , Boukhris Imed , Hann Sin Wei 
Published: August 2021

Abstract
In this research the melt quenching technique method was used to synthesize two series of borotellurite glass systems doped with manganese and strontium. Elastic measurement, X-Ray Diffraction and Fourier Transform Infrared spectroscopy were used to characterize the prepared glass samples. The increment of molar volume confirmed the theory that molar volume is inversely proportional to the density parameters. A broad hump appeared in XRD as the samples shown pure amorphous nature. In FTIR, the functional group vibrations of tellurite network were recorded such as TeO4 trigonal bipyramids and TeO3 trigonal pyramids by addition of both dopants. On the other hand, ultrasonic velocity was used to determine the elastic moduli of the glass such as bulk modulus, shear modulus, longitudinal modulus, Young’s Modulus, microhardness and Poisson’s ratio which showed decreasing and increasing trends with the increased concentration of MnO2 and SrO respectively.
The Analysis of the Intraoperative Complications During the Transitional Period from In-patient Cataract Surgery to Ambulatory Day Surgery
Authors: Shaochong Bu , Xiao-Rong Li , Shaozhen Zhao , Mengran Li , Qing Feng , Gao Fei , Ruibo Yang , Tian Fang 
Published: August 2021

Abstract
Purpose: To report the incidence of posterior capsular rupture (PCR) of phacoemulsification and the contributing factors during the transitional period from in-patient model to ambulatory day surgery model. Method: The medical records and intraoperative complication reports were systemically reviewed during the period of August 2015 to October 2020. The PCR rate was analyzed according to the following factors: type of surgery (day surgery vs. in-patient surgery), gender, age, surgery performed in the month containing long holiday (Chinese New Year and National day), surgery performed in the first month of the residents’ rotation, increase of surgical volume comparing to the previous month, stage of the day surgery transition and whether it was before or after the implementation of safety recommendation based on clinical audit results in January 2018. The univariable logistic regression model was initially performed.Results: Within the study period, 29 493 cases of phacoemulsification surgery were enrolled in the study, 14 451 of them were performed as day surgery while 15 042 of them were inpatient surgery. The overall incidence of PCR was 1.17% (346 cases) among the 29 493 planned phacoemulsification surgery. The increase incidence of PCR was associated with older age and male gender. The incidence of PCR decreased significantly after the implementation of safety recommendations. Conclusion: The ambulatory day surgery for cataract patients could provide safe, efficient, and quality services. During the transitional period from in-patient to day surgery, careful planning and organization with dynamic clinical audit surveillance can further reduce the incidence of intraoperative complications, especially intraoperative PCR.
MiR-29b suppresses peritoneal metastases through inhibition of the mesothelial-mesenchymal transition (MMT) of human peritoneal mesothelial cells
Authors: Kimura Yuki , Ohzawa Hideyuki , Miyato Hideyo , Kaneko Yuki , Takahashi Kazuya , Tojo Mineyuki , Yamaguchi Hironori , Kurashina Kentaro , Saito Shin , Hosoya Yoshinori , Lefor Alan Kawarai , Sata Naohiro , Kitayama Joji , Saito Akira 
Published: August 2021

Abstract
Background: Peritoneal dissemination is a major metastatic pathway for gastrointestinal and ovarian malignancies. The miR-29b family is downregulated in peritoneal fluids in patients with peritoneal metastases (PM). We examined the effect of miR-29b on mesothelial cells (MC) which play critical a role in the development of PM through mesothelial-mesenchymal transition (MMT).
Methods: Human peritoneal mesothelial cells (HPMCs) were isolated from surgically resected omental tissue and MMT induced by stimulation with 10 ng/ml TGF-b1. MiR-29b mimics and negative control miR were transfected by lipofection using RNAiMAX and the effects on the MMT evaluated in vitro.
Results: HPMC produced substantial amounts of miR-29b which was markedly inhibited by TGF-b1. TGF-b1 stimulation of HPMC induced morphological changes with decreased expression of E-cadherin and calretinin, and increased expression of vimentin and fibronectin. TGF-b1 also enhanced proliferation and migration of HPMC as well as adhesion of tumor cells in a fibronectin dependent manner. However, all events were strongly abrogated by simultaneous transfection of miR-29b.
Conclusion: MiR-29b inhibits TGF-b1 induced MMT and replacement of miR-29b in the peritoneal cavity might be effective to prevent development of PM partly through the effects on MC.
Kinetic studies relating to the accelerated expansion of the Universe
Authors: Zhang Wei , Deng Cheng 
Published: August 2021

Abstract
After taking into account the mass loss of galaxies and stars at the cosmic scale, the speed and acceleration of the accelerating expansion of the Universe are derived from general relativity and Newtonian mechanics, as respectively. The physical significance of the Hubble constant is proved to be the average of the masses ejected per second per unit mass in the observed range, and it is shown that the accelerated expansion of the universe doesn’t require dark energy
Palmöl in der EFTA-Indonesien Wirtschaftspartnerschaft
Authors: Sieber-Gasser Charlotte 
Published: August 2021

Den EFTA-Staaten ist in den Verhandlungen über eine Wirtschaftspartnerschaft mit Indonesien scheinbar Bahnbrechendes gelungen: Zum ersten Mal weltweit macht ein Handelsabkommen präferenzielle Zölle abhängig von der Art und Weise, wie ein Rohstoff produziert worden ist. Der — theoretische — ökonomische Anreiz zur nachhaltigen Produktion überwindet damit das Verbot in den WTO-Abkommen, zwischen nachhaltig und konventionell produzierten Waren zu unterscheiden. Allerdings zeigt ein näherer Blick, dass die gewährten Präferenzen keinen nennenswerten ökonomischen Wert besitzen. Dass diese Tatsache im Abstimmungskampf nicht offengelegt wurde, wirft Fragen auf: Was ist der Wert der Palmöl-Vereinbarung in der EFTA-Indonesien Wirtschaftspartnerschaft? Hat der Bundesrat seine Pflichten verletzt, indem er unvollständig über den Umfang des ökonomischen Anreizes zur nachhaltigen Palmölproduktion informiert hat? Dieser Beitrag verortet Handlungsbedarf in Bezug auf die Überwindung des Informationsvorsprungs der Bundesverwaltung gegenüber der Legislative im Meinungsbildungsprozess über internationale Handelsabkommen.
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Les États de l'AELE ont en apparence réalisé un exploit dans les négociations sur un partenariat économique avec l'Indonésie : pour la première fois au monde, un accord commercial fait dépendre l’octroi de tarifs préférentiels à la manière dont une matière première a été produite. Cette incitation économique - théorique - en faveur de la production durable permet ainsi de surmonter l'interdiction faite par les accords de l'OMC d'établir une distinction entre les biens produits de manière durable et ceux produits de manière conventionnelle. Toutefois, un examen plus approfondi montre que les avantages accordés n'ont aucune valeur économique appréciable. Le fait que cela n'ait pas été divulgué lors de la campagne référendaire soulève des questions : quelle est la valeur de l'accord sur l'huile de palme dans le cadre du partenariat économique AELE-Indonésie ? Le Conseil fédéral a-t-il violé ses devoirs en ne fournissant que des informations incomplètes sur l'ampleur de l'incitation économique à la production durable d'huile de palme ? Cet article identifie la nécessité d'agir pour surmonter l'avantage en matière d’information que possède l'administration fédérale sur législateur dans le processus de formation de l'opinion à propos des accords commerciaux internationaux.