bluuebunny/crossref_metadata_2025_split huggingface dataset:
Decoding the olfactory map: targeted transcriptomics link olfactory receptors to glomeruli
Authors: Zhu Kevin , Burton Shawn , Nagai Maira , Silverman Justin , De March Claire , Wachowiak Matt , Matsunami Hiroaki 
Published: November 2021

Abstract
Sensory processing in olfactory systems is organized across olfactory bulb glomeruli, wherein axons of peripheral sensory neurons expressing the same olfactory receptor co-terminate to transmit receptor-specific activity to central neurons. Understanding how receptors map to glomeruli is therefore critical to understanding olfaction. High-throughput spatial transcriptomics is a rapidly advancing field, but low-abundance olfactory receptor expression within glomeruli has previously precluded high-throughput mapping of receptors to glomeruli. Here we combined sequential sectioning along the anteroposterior, dorsoventral, and mediolateral axes with target capture enrichment sequencing to overcome low-abundance target expression. This strategy allowed us to spatially map 86% of olfactory receptors across the olfactory bulb and uncover a relationship between OR sequence and glomerular position.
A Novel Insight Into Mechanism of Derangement of Coagulation Balance: Interactions of Quantum Dots with Coagulation-Related Proteins
Authors: Zhang Lingyan , Wu Yingting , Luo Xingling , Jia Tianjiang , Li Kexin , Zhou Lihong , Mao Zhen , Huang Peili 
Published: November 2021

Abstract
Background
Quantum dots (QDs) have gained increased attention for their extensive biomedical and electronic products applications. Due to the high priority of QDs in contacting the circulatory system, understanding the hemocompatibility of QDs is one of the most important aspects for their biosafety evaluation. Thus far, the effect of QDs on coagulation balance haven’t been fully understood, and limited studies also have yet elucidated the potential mechanism from the perspective of interaction of QDs with coagulation-related proteins.
Results
QDs induced the derangement of coagulation balance by prolonging the activated partial thromboplastin time and prothrombin time as well as changing the expression levels of coagulation and fibrinolytic factors. The contact of QDs with PTM (prothrombin), PLG (plasminogen) and FIB (fibrinogen) which are primary coagulation-related proteins in the coagulation and fibrinolytic systems formed QDs-protein conjugates through hydrogen-bonding and hydrophobic interaction. The affinity of proteins with QDs followed the order of PTM>PLG>FIB, and was larger with CdTe/ZnS QDs than CdTe QDs. Binding with QDs not only induced static fluorescence quenching of PTM, PLG and FIB, but also altered their conformational structures. The binding of QDs to the active sites of PTM, PLG and FIB may promote the activation of proteins, thus interfering the hemostasis and fibrinolysis processes.
Conclusions
The interactions of QDs with PTM, PLG and FIB may be key contributors for interference of coagulation balance, that is helpful to achieve a reliable and comprehensive evaluation on the potential biological influence of QDs from the molecular level.
Willingness to Pay for Assisted Reproductive Technologies by Pastoral Herd Owners in Southern Rangelands of Kenya
Authors: Agutu Fredrick Odiwuor , Ondiek James Ombiro , Mbuku Samuel Mwanzia , Bebe Bockline Omedo 
Published: November 2021

Abstract
Uptake of Assisted Reproductive Technologies (ARTs) can widen and accelerate access to quality breeding stock under pastoral systems, but user participation without subsidy is uncertain when the herders have to meet the direct cost. Analysing willingness to pay (WTP) for ARTs may provide insights into potential uptake of ARTs when development agencies withdraw subsidies and private entrepreneurs take up the service delivery. This study estimated WTP for ARTs among 130 pastoral herd owners participating in Sahiwal cattle breed upgrading program implemented in Transmara Sub County in the southern rangelands of Kenya. The average WTP was KES 3,643 (USD 33.4), equivalent to 21.4% premium with reference to base price (KES 3,000) (USD 27.5). Sixty-seven percent of the pastoral herd owners expressed WTP above the hypothesized market price, twice more than those expressing WTP below the hypothesized market price (33%). The first choice attributes influencing the WTP for ARTs were high milk yield (62%), high value calves (37%), and high growth rates (1%). Gender was the only socioeconomic factor significantly (P<0.005) influencing WTP, with men expressing higher WTP than women (KES 3,870 (USD 35.5) versus KES 3,223 (USD 26.6). The results indicate a high likelihood of pastoral herd owners continuing to access and use ARTs at own cost, which provides opportunities for private sector participation in ARTs delivery and upscaling to increase access to superior Sahiwal genetic resources in pastoral herds. This will need putting in place policy interventions supportive of efficient ARTs delivery mechanisms.
Effects of Transcutaneous Electrical Nerve Stimulation on Myocardial Protection in Patients Undergoing Aortic Valve Replacement: A Randomized Clinical Trial
Authors: Cho Youn Joung , Jung Dhong-Eun , Nam Karam , Bae Jinyoung , Lee Seohee , Jeon Yunseok 
Published: November 2021

Abstract
BackgroundTranscutaneous electrical nerve stimulation (TENS) has been found to have cardioprotective effects. However, its effects on adult cardiac surgery patients remain unclear. We investigated the effects of TENS on myocardial protection in patients undergoing aortic valve replacement surgery using cardiopulmonary bypass.MethodsThirty patients were randomized to receive TENS or sham in three different anesthetic states – pre-anesthesia, sevoflurane, or propofol (each n = 5). TENS was applied at the upper arm for 30 min. Sham treatment was provided without nerve stimulation. The primary outcome was the difference in myocardial infarct size following ischemia-reperfusion injury in rat hearts perfused with pre- and post-TENS dialysate from the patients using Langendorff perfusion system.ResultsThere were no differences in myocardial infarct size between pre- and post-treatment in any group (41.4 ± 4.3% vs. 36.7 ± 5.3%, 39.8 ± 7.3% vs. 27.8 ± 12.0%, and 41.6 ± 2.2% vs. 37.8 ± 7.6%; p = 0.080, 0.152, and 0.353 in the pre-anesthesia, sevoflurane, and propofol groups, respectively).ConclusionsTENS did not have a cardioprotective effect in patients undergoing aortic valve replacement surgery.Trial registrationThis study was registered at clinicaltrials.gov (NCT03859115, on March 1, 2019).
A Protocol for a Market of Machine Learning Models
Authors: Popescu Claudiu Marius 
Published: November 2021

Abstract
This paper describes a protocol for a market of machine learning models. The economic interaction involves two types of agents: data providers- agents that have some data and want to use it to get a predictive model, and model providers- agents able to use the data to generate predictive models. First, we will show that the process is informationally asymmetric, therefore a standard direct market can not function. Then, we design a protocol with the aim of creating a viable and efficient market mechanism for these particular services, under the specific challenges of information asymmetries. The protocol is theoretically analysed, to establish it’s correctness and computational complexity. We also propose a simple reference implementation based on a HTTP API. The implementation is then used in a few case studies, and analysed empirically.
Effects of Hypocalcemic Vitamin D Analogs in the Expression of DNA Damage Induced in Minilungs from Hescs: Implications for Lung Fibrosis
Authors: Magro-Lopez Esmeralda , Chamorro-Herrero Irene , Zambrano Alberto 
Published: November 2021

Abstract

BackgroundIn our previous work, we evaluated the therapeutic effects of 1α,25-Dihydroxyvitamin D3, the biologically active form of vitamin D, in the context of bleomycin-induced lung fibrosis. Contrary to the expected, vitamin D supplementation increased DNA damage expression and cellular senescence in alveolar epithelial type II cells and aggravated the overall lung pathology induced in mice by bleomycin. These effects were probably due to an alteration of the cellular DNA double-strand breaks repair capability. In the present work we have evaluated the effects of two hypocalcemic vitamin D analogs (calcipotriol and paricalcitol) in the expression of DNA damage in the context of minilungs derived from human embryonic stem cells and in the cell line A549.ResultsAs in the case of the cell line A549, bleomycin can induce DNA damage in the generated minilungs enriched in alveolar cells. The results indicate that, in contrast to vitamin D, the treatment of the minilungs with the hypocalcemic analogs reduce significantly the bulk of DNA damage expression in both bidimensional arrays of epithelial cells (2D minilungs) and lung bud organoids (3D minilungs). The initial evaluation of a battery of commercially available vitamin D analogs shows a significant reduction in A549 cells of gH2AFX expression levels, a marker of DNA damage, cell senescence and aging.ConclusionsThe treatments based in hypocalcemic vitamin D analogs might be used to reduce the bulk of DNA damage and eventually the subsequent cell senescence expression that underlie lung conditions as those that can evolve with fibrosis.
Supply-Demand Matching of Medical Services in Haikou Under the Background of Hierarchical Diagnosis and Treatment - Based on Didi Travel Data
Authors: Shao Haiyan , Jin Cheng , Xu Jing , Zhong Yexi , Xu Bing 
Published: November 2021

Abstract
Background

Implementation of the Healthy China Strategy and the hierarchical diagnosis and treatment system has injected new vitality into medical services. Given the insufficient supply of medical services and increasing demand for medical treatment, exploring the supply-demand pattern of medical services has become an urgent theoretical and practical problem to be solved. The equity of healthcare facilities has received widespread attention, but due to limited data, there is little research on the supply-demand pattern of medical services. This study focuses on evaluating the supply-demand matching pattern of medical services at different levels in Haikou City with big geographic data and promoting the realization of a balance between medical supply and demand.
Methods

This study utilizes spatial data of medical institutions, didi travel data, and population density data. Firstly, use the two-step floating catchment area method and GIS spatial analysis to explore characteristics of the supply-demand patterns of medical services at different levels in Haikou. Secondly, based on didi travel data, mine residents' demand for medical treatment. Then combined with population density data, divide supply-demand matching of medical institutions into four types. Finally, propose optimization strategies for the problems.
Results

The accessibility pattern of high-level medical institutions in Haikou presents high in the north and low in the south. The accessibility pattern of low-level medical institutions is the opposite. High-level medical institutions have a strong need for medical treatment, which is less hampered by distance. The healthcare demand of low-level medical institutions is small, and they mainly are medium- and short-distance medical travel. The types of medical services at different levels are mainly "low supply - low demand" and "high supply - low demand" types.
Conclusions

Medical services at different levels in Haikou are mainly in supply-demand imbalance. Therefore, we put forward optimization strategies to promote the equity of primary medical services, such as propelling the establishment and improvement of the hierarchical diagnosis and treatment system, building a new model of medical and health service supply, and strengthening balanced coverage of primary medical institutions. The mining of big geographic data is beneficial to alleviate the contradiction between medical supply and demand, although the data and methods need to be improved.
Application of Multi-Attribute Decision-Making Methods for the Selection of Conveyor
Authors: Fulzele Satyam , Khatke Satywan , Kadam Shubham , Kamble Avinash 
Published: November 2021

Abstract
In the present time of innovation, conveyor assume an exceptionally indispensable part and have huge significance for material handling in different enterprises. A conveyor is essentially utilized for moving any sort of material from one area to other. It is made with nearer precisions, hence the expense related with manufacturing is additionally high. In this manner, it should work with better productivity. The choice of the best conveyor is a crucial activity for designers. Designers need to recognize different variables that will influence the functionalities of the conveyor system to limit bottlenecks in the system. An efficient methodology should be accomplished for the conveyor selection. Subsequently, the current work aims to the selection process of the best option for conveyor by using four decision making methods such as analytical hierarchy process, technique of order preference by similarity to ideal solution, compromise ranking method and Deng’s similarity based method. The selection is done among four alternatives based on six attributes viz: fixed cost each hour, variable cost each hour, conveyor speed, product width, product weight and flexibility. The analytical hierarchy process is used to determine weights of the attributes based on relative importance of each attribute. It is also observed that A3 conveyor is best suitable conveyor. Hence the above proposed strategies help decision-makers to examine and choose the best conveyor by considering the rank obtained of the alternatives.
The Role of Irinotecan-Bevacizumab as Rescue Regimen in Children With Low-Grade Gliomas: A Retrospective Nationwide Study in 72 Patients
Authors: de Marcellus Charles , Tauziède-Espariat Arnault , Cuinet Aurélie , Pasqualini Claudia , Robert Matthieu P. , Beccaria Kévin , Puget Stéphanie , Boddaert Nathalie , Figarella-Branger Dominique , De Carli Emilie , Bourdeaut Franck , Leblond Pierre , Fouyssac Fanny , Andre Nicolas , Bertozzi Anne I. , Butel Thibault , Dufour Christelle , Valteau-Couanet Dominique , Varlet Pascale , Grill Jacques 
Published: November 2021

Abstract
Introduction.
At least half of children with low-grade glioma (LGG) treated with first line chemotherapy experience a relapse/progression and may therefore need a second-line chemotherapy. Irinotecan-bevacizumab has been recommended in this setting in France after encouraging results of pilot studies. We performed a retrospective analysis to define the efficacy, toxicity and predictors for response to the combination on a larger cohort.
Methods.
We reviewed the files from children < 19 years of age with progressive or refractory LGG treated between 2009 and 2016 in 7 French centers with this combination.
Results.
72 patients (median age 7.8 years [range, 1-19]) received a median of 16 courses (range, 3-30). The median duration of treatment was 9 months (range, 1.4-16.2). 96% of patients experienced at least disease stabilization. The 6-month and 2-year progression-free survivals (PFS) were 91.7% [IC 95% 85.5-98.3] and 38.2% [IC 95% 28.2-51.8] respectively. No progression occurred after treatment in 18 patients with a median follow-up of 35.6 months (range, 7.6-75.9 months). Younger patients had a worse PFS (p=0.005). Prior chemoresistance, NF1 status, duration of treatment, histopathology or radiologic response did not predict response. The most frequent toxicities related to bevacizumab included grades 1-2 proteinuria in 21, epistaxis in 10, fatigue in 12 and hypertension in 8 while gastro-intestinal toxicity was the most frequent side effect related to irinotecan.
Conclusion.
Bevacizumab-irinotecan is highly effective for children with recurrent LGG who have failed standard chemotherapy regimens whatever their clinical characteristics, only younger children had a worse PFS.
WITHDRAWN: Effects of dietary energy and protein levels on growth curve parameters of Khazak native chickens
Authors: Faraji-Arough hadi , Ghazaghi Mahmoud , Kasmani Farzad Bagherzadeh , Rokouei Mohammad 
Published: November 2021

Abstract
This study was conducted to evaluate the effect of dietary energy (ME) and protein (CP) on growth parameters and absolute growth rates in the different ages of the Khazak chicks. A total of 360 one-day-old Khazak chicks were obtained from a local hatchery and in a 3×3 factorial experiment with completely randomized design, chicks were randomly allocated to experimental diets including 2,600, 2,800, and 3,000 kcal of ME/kg, and each containing 17, 19, and 21% CP from 7 to 98 days of age. Four growth model (Gompertz, Logistic, Lopez, and Richards) were fitted on weekly body weight data and the best model were selected by the goodness of fit criteria. Growth curve parameters were predicted for all chicks using the best model and other parameters including age (Ti) and weight (Wi) at the inflection point and absolute growth rate (AGR) in different ages were calculated from growth curve parameters. All parameters were analyzed using the general linear model procedure of SAS. Based on goodness of fit criteria, the Richards model had the lowest Akaike’s Information Criteria (AIC), root mean square error (RMSE), and highest adjusted determination coefficient (R2Adj) than other models and was selected as the best model. The effect of ME was significant on the mature index (k), Wi, Ti, and all AGR parameters (P<0.05) while CP levels were significant on final weight (Wf), Wi, and AGR parameters (P<0.05). The chicks fed with a diet containing 2,600 kcal of ME/kg and 17 % CP had the higher k parameter, and lower Wi, Ti, and AGR than those fed with other diets (P<0.05). Considering that the level of 2,800 kcal of ME/kg and 19 % CP had no significant difference with the level of 3,000 kcal of ME/kg and 21 % CP, therefore diet with 2,800 kcal of ME/kg and 19 % CP was suggested as optimum levels for change the growth curve parameter and having best performance for Khazak chickens during 7 to 98 days of old.
Massive Digital Gene Expression Analysis Reveals Different Predictive Profiles for Immune Checkpoint Inhibitor Therapy Between Adenocarcinoma and Squamous Cell Carcinoma of Advanced Lung Cancer
Authors: Kaneda Toshihiko , Kurata Takayasu , Yoshida Tomoko , Kibata Kayoko , Yoshioka Hiroshige , Yanagimoto Hiroaki , Takeda Kazuhiko , Yoshida Takao , Tsuta Koji 
Published: November 2021

Abstract
Background: Immune checkpoint inhibitors prolong the survival of non-small cell lung cancer (NSCLC) patients.Although it has been acknowledged that there is some correlation between the efficacy of anti-programmed cell death-1 (PD-1) antibody therapy and immunohistochemical analysis, this technique is not yet considered foolproof for predicting a favorable outcome of PD-1 antibody therapy. We aimed to predict the efficacy of nivolumab based on an exhaustive analysis of RNA expression at the gene level in advanced NSCLC.Methods: This was a retrospective study on patients with NSCLC who were administered nivolumab at the Kansai Medical University Hospital. To identify genes associated with response to anti-PD-1 antibodies, we grouped patients into responders (complete and partial response) and non-responders (stable and progressive disease) to nivolumab therapy. Significant genes were then identified for these groups using Welch’s t-test.Results: Among 42 analyzed cases (20 adenocarcinomas and 22 squamous cell carcinomas), enhanced expression of MAGE-A4,BBC3 and OTOA genes was observed in responders with adenocarcinoma, and enhanced expression of DAB2, HLA-DPB1and CDH2genes was observed in responders with squamous cell carcinoma.Conclusions: This study predicted the efficacy of nivolumab based on an exhaustive analysis of mRNA expression at the gene level in advanced NSCLC. We also revealed different gene expression patterns as predictors of the effectiveness ofanti PD-1 antibody therapy in adenocarcinoma and squamous cell carcinoma.
Quality of Life After Definitive Linear Accelerator-based Stereotactic Radiotherapy for Prostate Cancer: a Longitudinal Study
Authors: Yamashita Hideomi , Ogita Mami , Sawayanagi Subaru , Nozawa Yuki , Abe Osamu 
Published: November 2021

Abstract
Background: Prostate cancer is the second most common malignancy worldwide, and the majority of patients are diagnosed with localized disease. We examined patients’ quality of life after stereotactic body radiation therapy (SBRT) for prostate cancer. Method: We included patients who were treated between 2016 and 2020. Inclusion criteria were adenocarcinoma of the prostate; class risk of low, intermediate, and high; and a World Health Organization performance status of 0–2. Quality of life was measured using the Functional Assessment of Cancer Therapy-Prostate (FACT-P). Results: A total of 439 patients were treated with SBRT, with a median age of 73 years old. The median follow-up period was 34 months. FACT-P Trial Outcome Index (p < 0.0001), FACT-General (p = 0.0003), and FACT-P-Total (p < 0.0001) scores declined at 1 month post-SBRT, then recovered and returned to the same level as before treatment at 3–4 months post-SBRT. The decrease in quality of life in the first month was particularly remarkable in patients who received long-term hormone injections (36%). One month after the end of SBRT, about 22% of patients experienced "quite a bit” or more troubling side effects. Conclusions: This study showed longitudinal changes in quality of life by FACT-P after SBRT for prostate cancer. Overall, prostate SBRT was well tolerated.
Transcriptional Analysis and Target Genes Discovery of Pseudomonas Aeruginosa Biofilm Developed Ex Vivo Chronic Wound Model
Authors: Tan Xiaojuan , Cheng Xi , Hu Mei , Zhang Yifan , Jia Aiqun , Zhou Jinwei , Zhu Guoping 
Published: November 2021

Abstract
Bacterial biofilms formation is one of the major reasons for treatment failure in chronic wound infections. Therefore, diagnostic biomarkers remain the best option for prevention and treatment of chronic wound infections by biofilms. Herein, Pseudomonas aeruginosa PAO1 was used to mimic biofilm development in porcine skin explants wells as ex vivo wound model. The microscopic imaging showed that PAO1 in porcine skin explants wells formed micro-colonies at 24 h, developed mushroom-like structure at 48 h, and at 72 h mushroom-like structure disappeared, remaining a thin bacterial lawn. RNA-seq data analysis revealed that the expression levels of genes involved in the type II hxc secretion system were significantly higher in biofilms than in planktonic cells, especially the expression of lapA encoding alkaline phosphatase. However, the expression levels of genes associated with denitrification pathway were markedly decreased in biofilms, especially the transcription of nirS encoding nitrite reductase to produce nitric oxide (NO). Therefore, their expressions and products were further detected using RT-qPCR and biochemical assays, respectively. The results found that the expression of lapA and alkaline phosphatase activity were induced, but the expression of nirS and intracellular NO were reduced at the whole biofilms cycle. The study indicates that LapA and NO would play an important role for P. aeruginosa biofilm formation in chronic wound infections. LapA would serve as potential target to monitor chronic wound infections by P. aeruginosa biofilms. Inducing NO would be used to treat chronic wound infections due to P. aeruginosa biofilms.