Molecular epidemiology of SARS-CoV-2 isolated from COVID-19 family clusters
Authors: Gunadi  , Wibawa Hendra , Hakim Mohamad Saifudin , Marcellus  , Trisnawati Ika , Khair Riat El , Triasih Rina , Irene  , Afiahayati  , Iskandar Kristy , Siswanto  , Anggorowati Nungki , Daniwijaya Edwin Widyanto , Supriyati Endah , Nugrahaningsih Dwi Aris Agung , Budiono Eko , Retnowulan Heni , Puspadewi Yunika , Puspitawati Ira , Sianipar Osman , Afandy Dwiki , Simanjaya Susan , Widitjiarso William , Puspitarani Dyah Ayu , Fahri Fadil , Riawan Untung , Fauzi Aditya Rifqi , Kalim Alvin Santoso , Ananda Nur Rahmi , Setyati Amalia , Setyowireni Dwikisworo , Laksanawati Ida Safitri , Arguni Eggi , Nuryastuti Titik , Wibawa Tri ,   the Yogyakarta-Central Java COVID-19 study group, Herini Elisabeth S. , Widowati Titis , Satria Cahya Dewi , Sumardi  , Riyanto Bambang Sigit , Gani Munawar , Maulana Satria , Rizki Ludhang Pradipta , Intansari Umi Solekhah , Herningtiyas ‬Elizabeth Henny , Harahap Nur Imma Fatimah , Poermadjaja Bagoes , Hutasoit Sintong H. M. T. , Indaryati  , Setyawan Havid , Athollah Kemala , Inggriani Maria Patricia 
Published: June 2021

AbstractBackgroundTransmission within families and multiple spike protein mutations have been associated with the rapid transmission of SARS-CoV-2. We aimed to: (1) describe full genome characterization of SARS-CoV-2 and correlate the sequences with epidemiological data within family clusters, and (2) conduct phylogenetic analysis of all samples from Yogyakarta and Central Java, Indonesia and other countries.MethodsThe study involved 17 patients with COVID-19, including two family clusters. We determined the full-genome sequences of SARS-CoV-2 using the Illumina MiSeq next-generation sequencer. Phylogenetic analysis was performed using a dataset of 142 full-genomes of SARS-CoV-2 from different regions.ResultsNinety-four SNPs were detected throughout the open reading frame (ORF) of SARS-CoV-2 samples with 58% (54/94) of the nucleic acid changes resulting in amino acid mutations. About 94% (16/17) of the virus samples showed D614G on spike protein and 56% of these (9/16) showed other various amino acid mutations on this protein, including L5F, V83L, V213A, W258R, Q677H, and N811I. The virus samples from family cluster-1 (n = 3) belong to the same clade GH, in which two were collected from deceased patients, and the other from the survived patient. All samples from this family cluster revealed a combination of spike protein mutations of D614G and V213A. Virus samples from family cluster-2 (n = 3) also belonged to the clade GH and showed other spike protein mutations of L5F alongside the D614G mutation.ConclusionsOur study is the first comprehensive report associating the full-genome sequences of SARS-CoV-2 with the epidemiological data within family clusters. Phylogenetic analysis revealed that the three viruses from family cluster-1 formed a monophyletic group, whereas viruses from family cluster-2 formed a polyphyletic group indicating there is the possibility of different sources of infection. This study highlights how the same spike protein mutations among members of the same family might show different disease outcomes.