bluuebunny/crossref_metadata_2025_split huggingface dataset:
Nanobody-Mediated Inhibition of P2X7 on Microglia Improves Stroke Outcome
Authors: Wilmes Maximilian , Espinoza Carolina Pinto , Ludewig Peter , Liesz Arthur , Nicke Annette , Gelderblom Mathias , Gerloff Christian , Falzoni Simonetta , Tolosa Eva , Virgilio Francesco Di , Rissiek Björn , Plesnilla Nikolaus , Koch-Nolte Friedrich , Magnus Tim 
Published: November 2021

Abstract
BackgroundPrevious studies have demonstrated that purinergic receptors could be therapeutic targets to modulate the inflammatory response in multiple brain disease models. However, tools for the selective and efficient targeting of these receptors are scarce. The new development of P2X7-specific nanobodies (nbs) enables us to effectively block the P2X7-channel.MethodsTemporary middle cerebral artery occlusion (tMCAO) in wildtype and P2X7-transgenic mice was used as a model for ischemic stroke. ATP release was assessed in transgenic ATP sensor mice. Stroke size was measured without treatment and after injection of P2X7-specific nbs i.v. and i.c.v. directly before tMCAO-surgery. P2X7-GFP expressing transgenic mice were used to show immunhistochemically P2X7 distribution in the brain. In vitro cultured microglia were used to investigate calcium-influx, pore-formation via DAPI uptake, caspase 1 activation and IL-1b release after incubation with P2X7-specific nbs. ResultsATP sensor mice showed an increase of ATP-release in the ischemic hemisphere compared to the contralateral hemisphere or sham mice up to 24 h after stroke. We could further verify the role of the ATP-P2X7 axis in P2X7-overexpressing mice, which showed significantly greater stroke volumes after 24 h. In vitro experiments with primary microglia cells showed that P2X7-specific nanobodies were capable of dampening the ATP-trigged calcium-influx and formation of membrane pores measured by Fluo4 fluorescence or DAPI uptake. We found a lower caspase 1 activity and a subsequently lower IL-1b release. However, the intravenous (i.v.) injection of P2X7-specific nanobodies compared to isotype controls before the tMCAO-surgery did not result in smaller stroke size compared to isotype controls. As demonstrated by FACS, nbs had only reached brain infiltrating macrophages but not microglia. To reach microglia, we injected the P2X7-spezific nbs or the isotype directly intraventricularly (icv). 30 mg of P2X7-specific nbs proved efficient for microglial targeting, reducing post-stroke microglia activation and stroke size significantly.ConclusionHere, we demonstrate the importance of locally produced ATP for the tissue damage observed in ischemic stroke and we show the potential of icv injected P2X7-specific nbs to reduce ischemic tissue damage.
Preliminary report on landslide early warning on August 20, 2021,in Nangqian County, Qinghai Province, China
Authors: Wang Xiangpeng , Lin Fanqiang , Shen Di , Sun Zhiyong 
Published: November 2021

Abstract
Based on the analysis of its formation mechanism, stability state and development trend, this paper focuses on monitoring key indicators such as surface deformation and rainfall to realize early warning of landslide and provide some guidance for landslide disaster prevention and avoidance in the alpine mountainous area of Nangqian, Qinghai Province. The landslide of the Baima Elin Monetary has the topographic and geomorphological conditions of landslide formation, and has the basic characteristics of being influenced by rainfall and climate, so the warning of the landslide should be comprehensive. At the same time, the current situation of the landslide is in an unstable state, and the seasonal freeze-thaw cycle will cause its stability to weaken further. Through macroscopic signs, it is predicted that the deformation of Baima Elin Monetary landslide will continue to intensify without a sign of abatement, and the stability will be further reduced after winter, tending to develop in an unfavorable direction, and engineering measures should be taken as soon as possible to manage it.
Estimating the Influence of High Temperature on Hand, Foot and Mouth Disease Incidence in China
Authors: Luan Guijie , Liu Shaonan , Zhang Weiyan , Zhai Long , Zhang Yingjie , Sun Liang , Yao Hongyan 
Published: November 2021

Abstract
The burden of disease caused by ambient high temperature has become a public health concern, but the association between high temperature and hand, foot and mouth disease (HFMD) remain indistinct. We used Distributed Lag Non-linear Model (DLNM) to estimate the burden of disease attribute to high temperature, adjusting for long-term trend and weather confounders. Total 18167455 cases were reported in 31 Chinese provinces, the incidence of HFMD showed a gradually increasing trend from 2008 to 2017 in China. Minimum Mortality Temperature (MMT) was mainly concentrated at 17°C to 23°C in the age group less than 5 years old, 18°C to 25°C in the age group 6~10 years old and 19°C to 27°C in the age group above 10 years old. The greatest RR in age group 0~5 years old was 2.06 (95%CI: 1.85~2.30) in Heilongjiang, and the lowest RR was 1.02 (95%CI: 1.00~1.05) in Guangdong; the greatest RR in age group 6~10 years old was 2.24 (95%CI: 1.72~2.91) in Guizhou, and the lowest RR was 1.01 (95%CI: 0.97~1.12) in Tianjin; the greatest RR in the age group over 10 years old was 2.53 (95%CI: 1.66~3.87) in Heilongjiang, and the lowest RR 1.02 (95%CI: 0.71~1.46) in Henan. We found the positive association between high temperature and HFMD in China.
Simulating Epileptic Seizures using the Bidomain Model
Authors: Schreiner Jakob , Mardal Kent-Andre 
Published: November 2021

Abstract
Epileptic seizures are due to excessive and synchronous neural activity. Extensive modelling of seizures has been done on the neuronal level, but it remains a challenge to scale these models up to whole brain models. Measurements of the brain’s activity over several spatiotemporal scales follow a power-law distribution in terms of frequency. During normal brain activity, the power-law exponent is often found to be around 2 for frequencies between a few Hz and up to 150 Hz, but is higher during seizures and for higher frequencies. The Bidomain model has been used with success in modelling the electrical activity of the heart, but has been explored far less in the context of the brain. This study extends previous models of epileptic seizures on the neuronal level to the whole brain using the Bidomain model. Our approach is evaluated in terms of power-law distributions. The electric potentials were simulated in 7 idealized 2D models and 3 MRI-derived 3D patient-specific models. Computed electric potentials were found to follow power-law distribtions with slopes ranging from 2 to 5 for frequencies greater than 10-30 Hz.
A Global View of Observed Changes in Fire Weather Extremes: Uncertainties and Attribution To Climate Change.
Authors: Liu Zhongwei , Eden Jonathan M. , Dieppois Bastien , Blackett Matthew 
Published: November 2021

Abstract
In many parts of the world, wildfires have become more frequent and intense in recent decades, raising concerns about the extent to which climate change contributes to the nature of extreme fire weather occurrences. However, studies seeking to attribute fire weather extremes to climate change are hitherto relatively rare and show large disparities depending on the employed methodology. Here, an empirical-statistical method is implemented as part of a global probabilistic framework to attribute recent changes in the likelihood and magnitude of extreme fire weather. The results show that the likelihood of climate-related fire risk has increased by at least a factor of four in approximately 40% of the world’s fire-prone regions as a result of rising global temperature. In addition, a set of recent fire weather events, occurring during a recent 5-year period (2014-2018) and identified as exceptional due to the extent to which they exceed previous maxima, are, in most cases, associated with an increase likelihood resulting from rising global temperature. The study’s conclusions highlight important uncertainties and sensitivities associated with the selection of indices and metrics to represent extreme fire weather and their implications for the findings of attribution studies. Among the recommendations made for future efforts to attribute fire weather extremes is the consideration of multiple fire weather indicators and communication of their sensitivities.
Complete Genome Sequence and Phylogenetic Analysis of Red Seabream Iridovirus Isolated From a Cage-Cultured Small Yellow Croaker (Larimichthys Polyactis) in China
Authors: Wang Gengshen , Xu Wei , Cheng Haoxue , Xie Jianjun , Bai Aixu , Xu Wenjun , Liang Qizhang 
Published: November 2021

Abstract
Iridoviruses are emerging pathogens widely spread in diverse environments and hosts. Numerous species of the iridoviridae family are known to cause severe disease in freshwater and marine organisms. Here, we report the complete genome sequence and phylogenetic analysis of the iridovirus strain LPIV-ZS-2021 isolated from a small yellow croaker (Larimichthys polyactis) in China. The genome sequence comprises 110,560 bp with a G+C content of 53.42%, has 104 putative open reading frames (ORFs) and shares highest sequence homology with Red red seabream iridovirus (RSIV) isolated in Japan (98.61%). Phylogenetic analysis revealed that it is clustered within RSIV clade1. This is the first fully sequenced RSIV genomes detected in the small yellow croaker. The host range expansion of Megalocytivirus genus iridovirus warrants further attention to determine its potential economic and ecological impact.
Packaging Composite Films Fabricated from Cellulose Pulp with Polyurethane and Curcumin by Using NMMO ionic Liquid Solvent
Authors: Jo Chaehyun , Kim Sam Soo , Ramalingam Srinivasan , S Prabakaran D. , Rukmanikrishnan Balasubramanian , Lee Jaewoong 
Published: November 2021

Abstract
Cellulose pulp (CP), polyurethane (PU), and curcumin-based biocompatible composite films were prepared using a simple cost-effective method. These materials dissolved well in the ionic liquid solvent N-methylmorpholine N-oxide. Significant structural and microstructural changes were observed in CP upon the addition of PU. These changes were studied using Fourier transform infrared spectroscopy, X-ray diffraction, and scanning electron microscopy. The 5% and 10% gravimetric losses of the CP/PU/curcumin composite were found to be in the range 87.2–182.3 ºC and 166.7–249.8 ºC, respectively. The addition of PU significantly improved the thermal stability and water barrier properties of the composites. All the composites exhibited single Tg values in the range 147.4–154.2 ºC. The tensile strength of CP was measured to be 93.2 MPa, which dropped to 14.1 MPa for the 1:0.5 CP/PU composite and then steadily increased to 30.5 MPa with further addition of PU. The elongation at break of the composites decreased from 8.1 to 3.7% with the addition of PU. The addition of PU also improved the water vapor permeability (3.96 ×10–9 to 1.75 ×10–9 g m–1 s–1 Pa–1) and swelling ratio (285 to 202%) of the CP composite films. The CP/PU/curcumin composite exhibited good antioxidant activity and no cytotoxicity when tested on the HaCat cell line. The visual appearance and UV transmittance (86.2–32.9% at 600 nm) of the CP composite films were significantly altered by the incorporation of PU and curcumin. This study demonstrates that CP/PU/curcumin composites can be used for various packaging and biomedical applications.
Lightning Evolution and VLF Perturbations Associated With Category 5 TC Yasa in the South Pacific Region
Authors: Redoblado Paolo , Kumar Sarwan , Kumar Abhikesh , Kumar Sushil 
Published: November 2021

Abstract
In this paper, we present the D-region ionospheric response during the lifespan (10–19 December 2020) of a severe category 5 tropical cyclone (TC) Yasa in the South Pacific by using the very low frequency (VLF, 3-30 kHz) signals from NPM, NLK, and JJI transmitters recorded at Suva, Fiji. Results indicate enhanced lightning and convective activity in all three regions (eyewall, inner rainbands, and outer rainbands) during the TC Yasa that are also linked to the wave sensitive zones of these transmitter-receiver great circle paths. Of the three regions, the outer rainbands showed the maximum lightning occurrence; hence convective activity. Prominent eyewall lightning was observed just before the TC started to weaken following its peak intensity. Analysis of VLF signal amplitudes showed both negative and positive perturbations (amplitudes exceeding ±3σ mark) lasting for more than 2 hours with maximum change in the daytime and nighttime signal amplitudes of -4.9 dB (NPM) and -19.8 dB (NLK), respectively. The signal perturbations were wave-like, exhibiting periods of oscillations between ~2.2-5.5 hours as revealed by the Morlet wavelet analysis. Additionally, the LWPC modeling of the signal perturbations indicated a 10 km increase in daytime D-region reference height, H¢, and a 12 km decrease in nighttime D-region H¢ during TC Yasa. The D-region density gradients (sharpness), b, showed small perturbations of 0.01–0.14 km-1 from its normal values. We suggest that the observed changes to the D-region parameters are due to the enhanced convection during TC Yasa which excites atmospheric gravity waves producing travelling ionospheric disturbances to the D-region.
Chrysophanol Inhibits the Progression of Gastric Cancer by Activating NLRP3
Authors: Binfen Hou , Zhao Li , Deng Min 
Published: November 2021

Abstract
AimGastric cancer is one of the most common malignant tumors.Chrysophanol has been reported to have antitumor effects on a variety of cancers, but the role of chrysophanol in gastric cancer remains unclear. The aim of this study was to investigate the effects of chrysophanol on proliferation, pyroptosis, migration and invasion of gastric cancer cells.MethodsMKN 28 and AGS cells were treatde with different concentrations of chrysophanol, then cell proliferation, migration,invasion and pyroptosis were decteed by CCK-8, Colony-forming assay, Wound Healing assay, Transwell and flow cytometry, respectively.Subsequently, NLRP3 siRNA was transfected into MKN 28 cells, cell proliferation pyroptosis, migration and invasion were reassessed in these transfected cells. The expression of caspase-1 and IL-1β in the downstream of NLRP3 was detected by qRT PCR and Western blot.ResultsChrysophanol significantly inhibited the proliferation of GC cells, promoted pyroptosis, inhibited cell migration and invasion, and up-regulated the expression level of NLRP3 inflammasome in GC cells. Silencing NLRP3 inhibited the effects of chrysophanol on proliferation, pyroptosis, migration and invasion of MKN 28 cells. Chrysophanol plays an anti-cancer role through high expression of NLRP3.CoclusionsChrysophanol can inhibit the proliferation, migration and invasion of gastric cancer cells by regulating NLRP3, promote the death of gastric cancer cells, and play an anti-tumor role,which is a clinical strategy with great potential for the treatment of gastric cancer.
Effect of Reynoutria Japonic and Resveratrol on Sirt1/SOD/MDA of Adriamycin-Induced Renal Injury: A Randomised Trial
Authors: Cao Shangmei , liu xiaojing , sun xinxin , chen xixia , Tang shuifu 
Published: November 2021

Abstract
Background: Mechanisms of drug-induced kidney injury include mitochondrial dysfunction and oxidative stress. Resveratrol is a natural activator of sirt1 that is related to oxidative stress.Objectives: To explore the mechanism of treating drug-induced kidney injury with Reynoutria japonica and its extract (Resveratrol).Methods: Fifty adult male SD rats were randomly divided into five groups: blank group, model group, Reynoutria japonica group, resveratrol group and Benazepril group. Except the blank group, each group used a one-time tail vein injection of 7.5mg/kg adriamycin to make the rat model of drug-induced renal injury. After three days, the proteinuria test strip showed green, which was positive for proteinuria. Each group was given the corresponding drug. ACR was measured on the seventh day every week. All rats were anaesthetized death on the fourth weekend to obtain blood and kidneys. Results: At the fourth week, the MDA levels of blank group and Reynoutria japonica group were significantly lower than those of benazepril hydrochloride group (P<0.05), and the MDA levels of resveratrol group and model group were significantly higher than those of benazepril hydrochloride group (P<0.05).The Sirt1 mRNA levels of the blank group and the Reynoutria japonica group were significantly higher than those in the benazepril hydrochloride group (P<0.05), and the Sirt1 mRNA levels of resveratrol group and model group were significantly lower than those of the benazepril hydrochloride group (P<0.05). The comparison results between groups of the Sirt1 protein expression were the same as those of the Sirt1 mRNA expression (P<0.05).Conclusions: The therapeutic effect of the Reynoutria japonica group was better than that of the Benazepril group and resveratrol group.
Serum Metabolomic Signature Predicts Ovarian Response to Controlled Stimulation
Authors: MU XIN , Pei Mei-li , Zhu Feng , Fan Ya-juan , Shi Juan-zi , Liu Pei-jun 
Published: November 2021

Abstract
Introduction In in vitro fertilization (IVF) cycles, some patients fail to adequately respond to ovarian stimulation. Finding novel biomarkers predicting ovarian response in advance would be meaningful.Objective To identify serum metabolomics predicting the growth of follicles after controlled ovarian stimulation (COS).Methods Blood samples were collected at the start of pituitary downregulation and on the fifth day after controlled ovarian stimulation. Liquid chromatography–tandem mass spectrometry (LC-MS/MS) methods were used to quantify metabolites. Demographic data were calculated with SPSS version 22.0 software. Multivariate statistics were used to analyze metabolomics dataset. A receiver operating characteristic (ROC) curve was used to evaluate the diagnostic model.Results The number of retrieved oocytes was higher in the group of Follicle-to-oocyte index (FOI) ≥ 1 group. Analyses revealed 50 different metabolomics between the pre- and post- COS groups. Compared with baseline, amino acids increased significantly following COS. At baseline, acetylglycine was more abundant in the FOI <1 group, while glycine and lipids were more abundant in the FOI ≥1 group. After COS, glycine, N-acetyl-L-alanine, D-alanine, and 2-aminomuconic acid were increased in those with FOI ≥1, but L-glutamine was increased in those with FOI <1. ROC curves indicated that the combination of glycine, acetylglycine and lipids predicts different responses to controlled ovarian stimulation (AUC =0.866).Conclusion Serum metabolism might reflect the response to ovarian stimulation. Higher glycine and PC may be a good predictor for response to COS.
A lignan from Alnus japonica inhibits glioblastoma tumorspheres by suppression of FOXM1
Authors: Shim Jin-Kyoung , Lim Seung Hoon , Jeong Ji Hye , Choi Ran Joo , Oh Yoojung , Park Junseong , Choi Sunghee , Hong Junpyo , Kim Seo Jin , Moon Ju Hyung , Kim Eui-Hyun , Teo Wan-Yee , Chang Jong Hee , Ryu Jae-Ha , Kang Seok-Gu 
Published: November 2021

Abstract
Introduction

Glioblastoma (GBM) is the most common and aggressive malignant brain tumor. Forkhead Box M1 (FOXM1) has been shown to regulate cell proliferation, apoptosis, angiogenesis, DNA damage repair and tumorigenesis. The lignin, (−)-(2R,3R)-1,4-O-diferuloylsecoisolariciresinol (DFS), from Alnus japonica (Betulaceae) has shown anti-cancer effects against colon cancer cells by suppressing FOXM1. However, the efficacy of DFS in GBM has not yet been determined. The present study hypothesized that DFS can have anti-cancer effects against GBM tumorspheres (TSs).
Methods

Immunoprecipitation and luciferase reporter assays were performed to evaluate the ability of DFS to suppress nuclear translocation of β-catenin through β-catenin/FOXM1 binding. GBM TSs were treated with DFS to assess the ability of DFS to inhibit GBM TSs and to evaluate their transcriptional profiles. The in vivo efficacy of DFS was examined in orthotopic xenograft models of GBM.
Results

Expression of FOXM1 was higher in GBM than in normal tissues. DFS-induced FOXM1 protein degradation blocked β-catenin translocation into the nucleus and consequently suppressed downstream target genes of FOXM1 pathways. DFS considerably inhibited cell viability and ATP levels in GBM TSs, while increasing the proportion of apoptotic cells. Treatment with DFS also reduced neurosphere formation and the invasive properties of GBM TSs. Transcriptome analyses showed that DFS reduced the activities of transcription factors related to tumorigenesis, stemness, and invasiveness. In addition, DFS significantly inhibited tumor growth and prolonged the survival rate of mice in orthotopic xenograft models of GBM.
Conclusions

DFS inhibits the proliferation of GBM TSs by suppressing FOXM1. These findings suggest that DFS may be a potential therapeutic agent to treat patients with GBM.
METTL3 Promotes Lung Adenocarcinoma Tumorigenesis and Inhibits Ferroptosis by Stabilizing SLC7A11 m6A Modification
Authors: Xu Yiming , Lv Dandan , Yan Chao , Su Hua , Zhang Xue , Shi Yangfeng , Ying Kejing 
Published: November 2021

Abstract
Background: N6-methyladenosine (m 6 A) has emerged as a significant regulator of the progress of various cancers. However, its role in lung adenocarcinoma (LUAD) remains unclear. Here, we explored the biological function and underlying mechanism of methyltransferase-like 3 (METTL3), the main catalyst of m 6 A, in LUAD progression. Methods: The expression of m 6 A, METTL3, YTHDF1 and SLC7A11 were detected by immunochemistry or/and online datasets in LUAD patients. The effects of METTL3 on LUAD cell proliferation, apoptosis and ferroptosis were assessed through in vitro loss-and gain-of-function experiments. The in vivo effect on tumorigenesis of METTL3 was evaluated using the LUAD cell xenograft mouse model. MeRIP-seq, RNA immunoprecipitation and RNA stability assay were conducted to explore the molecular mechanism of METTL3 in LUAD. Results: The results showed that the m 6 A level, as well as the methylase METTL3 were both significantly elevated in LUAD patients and lung cancer cells. Functionally, we found that METTL3 could promote proliferation and inhibit ferroptosis in different LUAD cell models, while METTL3 knockdown suppressed LUAD growth in cell-derived xenografts. Mechanistically, solute carrier 7A11 (SLC7A11), the subunit of system Xc - , was identified as the direct target of METTL3 by mRNA-seq and MeRIP-seq. METTL3-mediated m 6 A modification could stabilize SLC7A11 mRNA and promote its translation, thus promoting LUAD cell proliferation and inhibiting cell ferroptosis, a novel form of programmed cell death. Additionally, we demonstrated that YTHDF1, a m 6 A reader, was recruited by METTL3 to enhance SLC7A11 m 6 A modification. Moreover, the expression of YTHDF1 and SLC7A11 were positively correlated with METTL3 and m 6 A in LUAD tissues.Conclusions: These findings reinforced the oncogenic role of METTL3 in LUAD progression and revealed its underlying correlation with cancer cell ferroptosis; these findings also indicate that METTL3 is a promising novel target in LUAD diagnosis and therapy.