bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mitochondrial bioenergetics boost macrophage activation, promoting liver regeneration in metabolically compromised animals
Authors: Goikoetxea‐Usandizaga Naroa , Serrano‐Maciá Marina , Delgado Teresa C. , Simón Jorge , Fernández Ramos David , Barriales Diego , Cornide Maria E. , Jiménez Mónica , Pérez‐Redondo Marina , Lachiondo‐Ortega Sofia , Rodríguez‐Agudo Rubén , Bizkarguenaga Maider , Zalamea Juan Diego , Pasco Samuel T. , Caballero‐Díaz Daniel , Alfano Benedetta , Bravo Miren , González‐Recio Irene , Mercado‐Gómez Maria , Gil‐Pitarch Clàudia , Mabe Jon , Gracia‐Sancho Jordi , Abecia Leticia , Lorenzo Óscar , Martín‐Sanz Paloma , Abrescia Nicola G. A. , Sabio Guadalupe , Rincón Mercedes , Anguita Juan , Miñambres Eduardo , Martín César , Berenguer Marina , Fabregat Isabel , Casado Marta , Peralta Carmen , Varela‐Rey Marta , Martínez‐Chantar María Luz 
Published: September 2021

Abstract

Background and Aims
Hepatic ischemia–reperfusion injury (IRI) is the leading cause of early posttransplantation organ failure as mitochondrial respiration and ATP production are affected. A shortage of donors has extended liver donor criteria, including aged or steatotic livers, which are more susceptible to IRI. Given the lack of an effective treatment and the extensive transplantation waitlist, we aimed at characterizing the effects of an accelerated mitochondrial activity by silencing methylation‐controlled J protein (MCJ) in three preclinical models of IRI and liver regeneration, focusing on metabolically compromised animal models.

Approach and Results
Wild‐type (WT), MCJ knockout (KO), and Mcj silenced WT mice were subjected to 70% partial hepatectomy (Phx), prolonged IRI, and 70% Phx with IRI. Old and young mice with metabolic syndrome were also subjected to these procedures. Expression of MCJ, an endogenous negative regulator of mitochondrial respiration, increases in preclinical models of Phx with or without vascular occlusion and in donor livers. Mice lacking MCJ initiate liver regeneration 12 h faster than WT and show reduced ischemic injury and increased survival. MCJ knockdown enables a mitochondrial adaptation that restores the bioenergetic supply for enhanced regeneration and prevents cell death after IRI. Mechanistically, increased ATP secretion facilitates the early activation of Kupffer cells and production of TNF, IL‐6, and heparin‐binding EGF, accelerating the priming phase and the progression through G1/S transition during liver regeneration. Therapeutic silencing of MCJ in 15‐month‐old mice and in mice fed a high‐fat/high‐fructose diet for 12 weeks improves mitochondrial respiration, reduces steatosis, and overcomes regenerative limitations.

Conclusions
Boosting mitochondrial activity by silencing MCJ could pave the way for a protective approach after major liver resection or IRI, especially in metabolically compromised, IRI‐susceptible organs.
The immunogenomic landscape of resected intrahepatic cholangiocarcinoma
Authors: Carapeto Fernando , Bozorgui Behnaz , Shroff Rachna T. , Chagani Sharmeen , Solis Soto Luisa , Foo Wai Chin , Wistuba Ignacio , Meric‐Bernstam Funda , Shalaby Ahmed , Javle Milind , Korkut Anil , Kwong Lawrence N. 
Published: September 2021

Background and Aims
Cholangiocarcinoma (CCA) is a deadly and highly therapy‐refractory cancer of the bile ducts, with early results from immune checkpoint blockade trials showing limited responses. Whereas recent molecular assessments have made bulk characterizations of immune profiles and their genomic correlates, spatial assessments may reveal actionable insights.

Approach and Results
Here, we have integrated immune checkpoint‐directed immunohistochemistry with next‐generation sequencing of resected intrahepatic CCA samples from 96 patients. We found that both T‐cell and immune checkpoint markers are enriched at the tumor margins compared to the tumor center. Using two approaches, we identify high programmed cell death protein 1 or lymphocyte‐activation gene 3 and low CD3/CD4/inducible T‐cell costimulator specifically in the tumor center as associated with poor survival. Moreover, loss‐of‐function BRCA1‐associated protein‐1 mutations are associated with and cause elevated expression of the immunosuppressive checkpoint marker, B7 homolog 4.

Conclusions
This study provides a foundation on which to rationally improve and tailor immunotherapy approaches for this difficult‐to‐treat disease.
Improvements in the Synthesis of the Third‐Generation EGFR Inhibitor Osimertinib
Authors: Voulgari Pinelopi , Alivertis Dimitrios , Skobridis Konstantinos 
Published: September 2021

AbstractOsimertinib, a third generation potent and specific EGFR inhibitor is an important drug against many forms of cancer. It was synthesized by an improved and highly efficient protocol, revisiting the classical synthetic process and modifying parameters, such as solvents, temperature, reagents, and reaction time. A cost‐effective, environmentally friendly methodology for the synthesis of osimertinib was established, which gave shorter reaction times, saved labor by eliminating purification steps through column chromatography, and enhanced yields. Four of the seven steps in total, were proceeded quantitatively or almost quantitatively (ca. 98 %). This synthetic protocol provides a very high overall yield, up to 68 %. In addition, the entire approach enables the preparation of osimertinib analogues and could be extended in the synthesis of other structurally similar bioactive compounds.
Barriers to digital health services among people living in areas of socioeconomic disadvantage: Research from hospital diabetes and antenatal clinics
Authors: Freeman Toby , Fisher Matthew , Foley Kristen , Boyd Mark A. , Ward Paul R. , McMichael Gai , Zimmermann Anthony , Dekker Gustaaf 
Published: September 2021

AbstractIssue addressedWe sought to examine barriers to access to, use of, and benefits from digital health services in an area of socioeconomic disadvantage of Adelaide, Australia.MethodsWe conducted waiting room surveys in two hospital diabetes clinics and one hospital antenatal clinic in South Australia, and follow‐up telephone interviews with 20 patients. We examined the extent of access to, use of and benefits from digital health services, and what barriers people encountered. We undertook mixed methods, with quantitative descriptive analysis and qualitative analysis.ResultsThirty‐seven diabetes clinic patients (54% response rate) and 99 antenatal clinic patients (33% response rate) participated. Sixty‐two percent of the patients with diabetes and 27% of antenatal clinic patients had never used digital health services. Seventeen percent of patients with diabetes and 30% of antenatal clinic patients were hesitant users, and 22% of patients with diabetes and 44% of antenatal clinic patients were confident users. Barriers included struggling to afford the technology or to stay connected and a lack of trust in online health information. Potential benefits included feeling more empowered and complementing face‐to‐face care.ConclusionsThere are socioeconomic barriers to access, use of, and ability to benefit from digital health strategies that mean not everyone will be able to benefit from digital health services.So What?As COVID‐19 accelerates the shift towards digital health services, people experiencing socioeconomic disadvantage may be excluded. If barriers to access and use are not addressed, they will exacerbate already increasing health inequities.
COVID‐19 vaccination strategies and policies in India: The need for further re‐evaluation is a pressing priority
Authors: Rackimuthu Sudhan , Hasan Mohammad Mehedi , Bardhan Mainak , Essar Mohammad Yasir 
Published: September 2021

AbstractIndia is one of the worst‐hit nations by the COVID‐19 pandemic and witnessed a devastating impact across cities in the country. Although behavioral measures like wearing a face mask, maintaining social distance, and hand hygiene helped to control the spread of the disease initially, but a long‐term action by vaccinating the population is a promising solution. On 16 January 2021, India undertook the challenge to vaccinate 300 million people by August 2021 against COVID‐19, the largest vaccination campaign globally. India has been lauded by several prominent organizations around the world for its efforts. But catering to India's massive population is not without its own set of complex challenges. As of 29 July 2021, a mere 9.82 million (approximately 7.03 percent of the total Indian population) people have been fully vaccinated against COVID‐19 with the first and second dose, and only 352.5 million (roughly 25.28 percent of the total Indian population) have been partly vaccinated with the first dose. This shows, India's current COVID‐19 vaccination policies and plans are still inadequate and not undisputedly equitable even after several amendments in the guidelines. However, even with the second wave abating slowly and steadily in India, there is a need to further re‐strategize the current vaccination policy and plans in India against COVID‐19 to help achieve long‐term positive outcomes in the shortest feasible time frame hoping to evade a third wave.
COVID‐19 pandemic responses of Canada and United States in first 6 months: A comparative analysis
Authors: Combden Shianne , Forward Anita , Sarkar Atanu 
Published: September 2021

AbstractIntroductionCanada and the United States have distinct health care and social policies, and it is important to see how they had been responding to the ongoing COVID‐19 pandemic.MethodsThe study period was limited to the first 6 months of the pandemic and aimed to explore the responses by public health authorities, media, general population, and law makers during the initial phase of pandemic.ResultsSocial disparity, underfunded pandemic preparation, and the initial failure to act appropriately have resulted in the rapid spread of infection in both countries. In the United States, prevailing social inequalities and racism, inaccessible health care, higher rates of preexisting medical conditions and disputed political leadership have further deteriorated the situation and enhanced public suffering, particularly for the black and Indigenous communities. In Canada, its poorly regulated services of long‐term care facilities, initial restriction of testing and lack of access to epidemiological data have helped spread the infection and increased casualties in vulnerable populations.ConclusionAnalysis of the pandemic responses of the United States and Canada has revealed how existing social disparity, underfunded pandemic preparation, and the initial failure to act appropriately have resulted in the rapid spread of infection.
Remote vital signs data monitoring during home blood transfusion: A pilot study
Authors: Nishikawa Akinori , Fujimori Yoshihiro , Sakano Noriko , Mushino Toshiki , Tamura Shinobu , Kasahara Shingo , Akasaka Hiroshi , Sonoki Takashi 
Published: September 2021

AbstractBackground and aimsOur study aimed to establish safer methods to manage home blood transfusion by using a remote vital signs data monitoring system. Home care is administered for patients with various medical disorders; however, home blood transfusion remains challenging owing to the risk of transfusion‐related complications.MethodsWe set up a remote vital signs data monitoring system to improve the safety of home blood transfusions. Using an Internet‐based vital signs data monitoring system, the heart rate, electrocardiography, respiration rate, and percutaneous oxygen saturation (SpO2) were monitored and recorded during the entire home transfusion period.ResultsTen transfusions in three patients were monitored; two of the patients had an abnormality in a single vital sign (decreased SpO2 decrease and increased respiratory rate); these were not transfusion‐related complications. Vital sign anomalies also occur because of errors in using the measurement device and noise associated with body movements. The presence of abnormalities in at least two vital signs among SpO2 decrease, tachycardia, and increased respiratory rate that persisted for >5 minutes was defined as a complicated vital sign abnormality (CVSA). There were no severe transfusion‐related complications with CVSA in the present study.ConclusionThis study indicates the feasibility and sustainability of real‐time remote monitoring of vital signs for the safety of home transfusion. Although CVSA may function as an indicator of severe transfusion‐related complications, these findings need to be confirmed with further studies.
Fluvial suspended sediment transfer and lacustrine sedimentation of recent flood turbidites in proglacial Eklutna Lake, western Chugach Mountains, Alaska
Authors: Schiefer Erik , Geck Jason , Ostman Johnse S. , McKay Nicholas P. , Praet Nore , Loso Michael G. , Kaufman Darrell S. 
Published: September 2021

AbstractSuspended sediment delivery and deposition in proglacial lakes is generally sensitive to a wide range of hydrometeorologic and geomorphic controls. High discharge conditions are of particular importance in many glaciolacustrine records, with individual floods potentially recorded as distinctive turbidites. We used an extensive network of surface sediment cores and hydroclimatic monitoring data to analyse recent flood turbidites and associated sediment transfer controls over instrumental periods at Eklutna Lake, western Chugach Mountains, Alaska. Close to a decade of fluvial data from primary catchment tributaries show a dominating influence of discharge on sediment delivery, with various interconnections with other related hydroclimatic controls. Multivariate fluvial models highlight and help quantify some complexities in sediment transfer, including intra‐annual variations, meteorological controls, and the influence of subcatchment glacierization. Sediments deposited in Eklutna Lake during the last half century are discontinuously varved and contain multiple distinctive turbidites. Over a 30‐year period of stratigraphic calibration, we correlate the four thickest flood turbidites (1989, 1995, 2006, and 2012) to specific regional storms. The studied turbidites correlate with late‐summer and early‐autumn rainstorms with a magnitude of relatively instantaneous sedimentation 3–15 times greater than annual background accumulation. Our network of sediment core data captured the broad extent and sediment variability among the study turbidites and background sediment yield. Within‐lake spatial modelling of deposition quantifies variable rates of downlake thinning and sediment focusing effects, and highlights especially large differences between the thickest flood turbidites and background sedimentation. This we primarily relate to strongly contrasting dispersion processes controlled by inflow current strength and turbidity. Sediment delivery is of interest for this catchment because of reservoir and water supply operations. Furthermore, although smaller floods may not be consistently represented, the lake likely contains a valuable proxy record of regional flooding proximal to major population centers of south‐central Alaska including Anchorage.
Long‐term precipitation and stream discharge records at seven forested experimental watersheds along a latitudinal transect in Japan: Jozankei, Kamabuchi, Takaragawa, Tsukuba, Tatsunokuchi‐yama, Kahoku and Sarukawa
Authors: Shimizu Takanori , Tamai Koji , Hosoda Ikuhiro , Noguchi Shoji , Kominami Yuji , Abe Toshio , Kitamura Kenzo , Kurokawa Ushio , Levia Delphis F. , Kubota Tayoko , Kabeya Naoki , Iida Shin'ichi , Nobuhiro Tatsuhiko , Sawano Shinji , Iwagami Sho , Shimizu Akira , Tsuboyama Yoshio 
Published: September 2021

AbstractThis data note introduces a database of long‐term daily total precipitation and stream discharge data for seven forested watersheds in Japan that have been continuously monitored by the Forestry and Forest Products Research Institute. Three of the watersheds started data collection in the 1930s. Forest cover across the sites ranges from cool to warm temperate regions with the latitude spanning from 31 to 44° N and annual precipitation ranging from 1200 to 3000 mm yr−1. The effects of vegetation change via clearcutting, thinning and forest fire (among other stressors) on stream discharge can be analysed from the long‐term observation sites. Moreover, this multi‐site dataset allows for inter‐ and intra‐site comparisons of annual water loss (difference of annual precipitation and stream discharge). These long‐term datasets can provide comprehensive insights into the effects of climate change and other stressors on forested ecosystems, not only in Japan but across a spectrum of forest types, if combined with other long‐term records from other forested watersheds across the world.
A synthetic review of terrestrial biological research from the Alberta oil sands region: 10 years of published literature
Authors: Roberts David R. , Bayne Erin M. , Beausoleil Danielle , Dennett Jacqueline , Fisher Jason T. , Hazewinkel Roderick O. , Sayanda Diogo , Wyatt Faye , Dubé Monique G. 
Published: September 2021

Abstract
In the past decade, a large volume of peer-reviewed papers has examined the potential impacts of oil and gas resource extraction in the Canadian oil sands (OS). A large proportion focuses on terrestrial biology: wildlife, birds, and vegetation. We provide a qualitative synthesis of the condition of the environment in the oil sands region (OSR) from 2009 to 2020 to identify gaps and progress cumulative effects assessments. Our objectives were to (1) qualitatively synthesize and critically review knowledge from the OSR; (2) identify consistent trends and generalizable conclusions; and (3) pinpoint gaps in need of greater monitoring or research effort. We visualize knowledge and terrestrial monitoring foci by allocating papers to a conceptual model for the OS. Despite a recent increase in publications, focus has remained concentrated on a few key stressors, especially landscape disturbance, and a few taxa of interest. Stressor and response monitoring is well represented, but direct monitoring of pathways (linkages between stressors and responses) is limited. Important knowledge gaps include understanding effects at multiple spatial scales, mammal health effects monitoring, focused monitoring of local resources important to Indigenous communities, and geospatial coverage and availability, including higher attribute resolution in human footprint, comprehensive land cover mapping, and up-to-date LiDAR coverage. Causal attribution based on spatial proximity to operations or spatial orientation of monitoring in the region is common but may be limited in the strength of inference that it provides. Integr Environ Assess Manag 2022;18:388–406. © 2021 The Authors. Integrated Environmental Assessment and Management published by Wiley Periodicals LLC on behalf of Society of Environmental Toxicology & Chemistry (SETAC).
KEY POINTS
We provide a qualitative synthesis of the condition of the environment in the Canadian oil sands region (OSR) in northeastern Alberta from 2009 to 2020 to identify gaps and progress cumulative effects assessments. Despite a recent increase in publications, focus has remained concentrated on a few key stressors and a few taxa of interest, for which monitoring is well represented, though direct monitoring of pathways (linkages between stressors and responses) is limited. Important gaps include a lack of understanding of effects at multiple spatial scales, a lack of focused monitoring of local resources important to Indigenous communities, and geospatial data resolution and availability. Causal attribution based on spatial proximity to oil sands operations or spatial orientation of monitoring in the OSR is common but may be limited in the strength of inference that it provides.
Chirality and Supramolecular Copolymerizations – The Elusive Role of Subtle Solvation Effects
Authors: Preuss Marco D. , Jansen Stef A. H. , Vantomme Ghislaine , Meijer E. W. 
Published: September 2021

AbstractRecent investigations of supramolecular polymers based on chiral triphenylene‐2,6,10‐tricarboxamides (TTAs) showed how temperature‐induced changes in solvation can greatly influence the preferred helical conformation of the supramolecular polymers formed. Here, we combine chiral TTA with achiral copolymerization partners to further investigate temperature‐dependent solvation effects. Systematic variation of the system's composition shows clear impacts on the temperature window where the conformational change occurs. Further, simple chain length variations in the achiral comonomer greatly affect the ability to influence the conformational change in the copolymer, while the differences in the properties of the individual homopolymers are rather small. We herein highlight how subtle changes in the monomers can impact the observed copolymer properties drastically; an effect arising from the emerging complexity of multicomponent interactions in supramolecular copolymers with solvent‐solute interactions being more important than typically thought.
Adnexal masses: Diagnostic performance of contrast‐enhanced ultrasound using the simple rules from the International Ovarian Tumor Analysis group
Authors: Xu Ailing , Nie Fang , Liu Ting , Dong Tiantian , Bu Lan , Yang Dan 
Published: September 2021

AbstractObjectiveTo evaluate the role of the Simple Rules (SR) from the International Ovarian Tumor Analysis group in contrast‐enhanced ultrasound (CEUS) of for the differential diagnosis of benign and malignant adnexal tumors.MethodsA total of 180 patients with suspected malignant adnexal masses admitted to Lanzhou University Second Hospital were included, all of whom received conventional ultrasound (US) and CEUS examination before surgery. All masses were assessed using SR in US and SR in CEUS. To compare the diagnostic performance, the sensitivity, specificity, negative and positive predictive values, Youden index, and area under the curves values of SR in US and SR in CEUS were obtained.ResultsSensitivity, negative predictive values, Youden index, and area under the curves were better for SR in CEUS than SR in US. The specificity and negative predictive value of SR in CEUS were lower than that of SR in US. Compared with SR in US, SR in CEUS significantly reduced the number of uncertain masses (21, 36.7% vs. 66, 11.7%).ConclusionWe concluded that SR in CEUS performed well in the diagnosis of malignant adnexal masses. SR in CEUS is a supplement to SR in US.
Outcomes of cesarean delivery in obstetric patients with SARS‐CoV‐2 infection
Authors: Lankford Allison , Berger Jeffrey , Benjenk Ivy , Jackson Amanda , Ahmadzia Homa , Mazzeffi Michael 
Published: September 2021

SynopsisSARS‐CoV‐2 infection was not associated with increased mortality, but was associated with a modest increase in morbidity, including stillbirth, in cesarean delivery patients in Maryland, USA.
Toward absolute viability measurements for bacteria
Authors: Dunkers Joy P. , Iyer Hariharan , Jones Brynna , Camp Charles H. , Stranick Stephan J. , Lin Nancy J. 
Published: September 2021

AbstractWe aim to develop a quantitative viability method that distinguishes individual quiescent from dead cells and is measured in time (ns) as a referenceable, comparable quantity. We demonstrate that fluorescence lifetime imaging of an anionic, fluorescent membrane voltage probe fulfills these requirements forStreptococcus mutans. A random forest machine‐learning model assesses whether individualS. mutanscan be correctly classified into their original populations: stationary phase (quiescent), heat killed and inactivated via chemical fixation. We compare the results to intensity using three models: lifetime variables (τ1, τ2and p1), phasor variables (G, S) or all five variables, with the five variable models having the most accurate classification. This initial work affirms the potential for using fluorescence lifetime of a membrane voltage probe as a viability marker for quiescent bacteria, and future efforts on other bacterial species and fluorophores will help refine this approach.