bluuebunny/crossref_metadata_2025_split huggingface dataset:
Veillonella parvula: a strictly anaerobic bacterium with high efficacy for safe and specific tumor targeting and colonization
Authors: Kefayat Amirhosein , Ghahremani Fatemeh , Rostami Soodabeh 
Published: May 2021

AbstractBacterial cancer therapy has gained lots of attention in the past decade and is now considering a reliable option for the future. However, some concerns have limited its application into clinic settings like insufficient colonization of tumors and infectious origin of the currently used bacteria like Clostridium and Salmonella species, especially in cancer patients which exhibit different levels of immunocompromising. In the present study, Veillonella parvula (V. parvula) as a strictly anaerobic bacterium which has rarely identified as a pathogen in human, was administrated into 4T1 breast tumor-bearing mice. At first, 4T1 breast tumor-bearing BALB/c mice were injected with 107 bacteria intravenously, intraperitoneally, orally, or intratumorally. The best administration route according to tumor colonization and safety was selected. Then, the therapeutic effect of V. parvula administration was investigated according to the 4T1 breast tumor’s growth, metastasis, and tumor-bearing mice survival. Besides, histopathological evaluations were done to estimate microscopic changes at the inner of the tumor. V. parvula exhibited significant tumor-targeting and colonization efficacy, 24 h after intravenous administration and formed clustered colonies at the central region of the tumors. Although a negligible number of the bacteria were localized at normal organs, these organs became clear from the bacteria after 72 h, and no side effects or death were observed at the animals after intravenous administration of V. parvula. Although mean tumor volumes in the V. parvula treated group was lower than the control (~ 25.4%), their difference wasn’t statistically significant (P > 0.05). Despite significant tumor colonization (5500000:1 in comparison with normal organs after 72 h), V. parvula didn’t cause a significant therapeutic effect on the metastasis or survival time of tumor-bearing mice. Taking together, V. parvula is a completely safe and tumor-specific agent per se, without any genetic manipulation. Also, it exhibits high tumor penetration and colonization at the deep regions of the tumor.
Precursor abundance influences divergent antigen specific CD8+ T cell responses after Yersinia pseudotuberculosis foodborne infection
Authors: Zhang Yue , Qiu Zhijuan , Sheridan Brian S. , Bliska James B. 
Published: May 2021

AbstractPrimary infection of C57BL/6 mice with the bacterial pathogen Yersinia pseudotuberculosis elicits an unusually large H-2Kb-restricted CD8+ T cell response to the endogenous and protective bacterial epitope YopE69-77. To better understand the basis for this large response, the model OVA257-264 epitope was inserted into YopE in Y. pseudotuberculosis and antigen specific CD8+ T cells in mice were characterized after foodborne infection with the resulting strain. The epitope YopE69-77 elicited significantly larger CD8+ T cell populations in the small intestine, mesenteric lymph nodes (MLNs), spleen and liver between 7- and 30-days post infection, despite residing in the same protein and having a similar affinity for H-2Kb as OVA257-264. YopE-specific CD8+ T cell precursors were ~4.6 times as abundant as OVA-specific precursors in the MLNs, spleen and other lymph nodes of naïve mice, explaining the dominance of YopE69-77 over OVA257-264 at early infection times. However, other factors contributed to this dominance as the ratio of YopE-specific to OVA-specific CD8+ T cells increased between 7- and 30-days post infection. We also compared the YopE-specific and OVA-specific CD8+ T cells generated during infection for effector and memory phenotypes. Significantly higher percentages of YopE-specific cells were characterized as short short-lived effectors while higher percentages of OVA-specific cells were memory precursor effectors at day 30 post infection in spleen and liver. Our results suggest that a large precursor number contributes to the dominance and effector and memory functions of CD8+ T cells generated to the protective YopE69-77 epitope during Y. pseudotuberculosis infection of C57BL/6 mice.
Kinesin-3 mediated axonal delivery of presynaptic neurexin stabilizes dendritic spines and postsynaptic components
Authors: Oliver Devyn , Ramachandran Shankar , Philbrook Alison , Lambert Christopher M. , Nguyen Ken C. Q. , Hall David H. , Francis Michael M. 
Published: May 2021

AbstractThe functional properties of neural circuits are defined by the patterns of synaptic connections between their partnering neurons, but the mechanisms that stabilize circuit connectivity are poorly understood. We systemically examined this question at synapses onto newly characterized dendritic spines of C. elegans GABAergic motor neurons. We show that the presynaptic adhesion protein, neurexin/NRX-1, is required for stabilization of postsynaptic structure. We find that early postsynaptic developmental events proceed without a strict requirement for synaptic activity and are not disrupted by deletion of neurexin/nrx-1. However, in the absence of presynaptic NRX-1, dendritic spines and receptor clusters become destabilized and collapse prior to adulthood. We demonstrate that kinesin-3/UNC-104 delivers NRX-1 to presynaptic terminals and show that ongoing delivery is required for postsynaptic maintenance in mature animals. By defining the dynamics and temporal order of synapse formation events in vivo, we describe a mechanism for stabilizing mature circuit connectivity through neurexin-based adhesion.
Viral shedding and transmission after natural infection and vaccination in an animal model of SARS-CoV-2 propagation
Authors: Zeiss Caroline J. , Asher Jennifer L. , Wyk Brent Vander , Allore Heather G. , Compton Susan 
Published: May 2021

AbstractAt present, global immunity to SARS-CoV-2 resides within a heterogeneous combination of susceptible, naturally infected and vaccinated individuals. The extent to which viral shedding and transmission occurs on re-exposure to SARS-CoV-2 after prior natural exposure or vaccination is an emerging area of understanding. We used Sialodacryoadenitis Virus (SDAV) in rats to model the extent to which immune protection afforded by prior natural infection via high risk (inoculation; direct contact) or low risk (fomite) exposure, or by vaccination, influenced viral shedding and transmission on re-exposure. On initial infection, we confirmed that amount, duration and consistency of viral shedding were correlated with exposure risk. Animals were reinfected after 3.7-5.5 months using the same exposure paradigm. Amount and duration of viral shedding were correlated with re-exposure type and serologic status. 59% of seropositive animals shed virus. Previously exposed seropositive reinfected animals were able to transmit virus to 25% of naive recipient rats after 24-hour exposure by direct contact. Rats vaccinated intranasally with a related virus (Parkers Rat Coronavirus) were able to transmit SDAV to only 4.7% of naive animals after a 7-day direct contact exposure, despite comparable viral shedding. Observed cycle threshold values associated with transmission in both groups ranged from 29-36 cycles, however observed shedding was not a prerequisite for transmission. Results indicate that low-level shedding in both naturally infected and vaccinated seropositive animals can propagate infection in susceptible individuals. Extrapolated to COVID-19, our results suggest that continued propagation of SARS-CoV-2 by seropositive previously infected or vaccinated individuals is possible.
Leader’s strategy to encourage employee’s innovative work behavior in multicultural workplace: do supportive colleagues matter?
Authors: Usmanova Kamila , Wang Daoping , Younas Amjad 
Published: May 2021

PurposeIn recent years, China’s growing global economic influence has attracted more foreign workers, requiring leaders to have effective communication skills to manage diverse personnel to drive innovations. Although previous research studies revealed the effects of a leader’s motivating language (ML) on employee’s innovativeness, the mechanism and the boundary conditions for stimulating the relationship between ML and innovative work behavior (IWB) are scarce. Therefore, this study aims to examine employee’s creative self-efficacy (CSE) as a mechanism and coworker support (CS) as a boundary condition in the relationship between ML’s dimensions and IWB.Design/methodology/approachTo test the moderated mediation model, this study collected the data from 283 workers and their respective supervisors at a Beijing-based multinational network company. The research applied a quantitative approach. SPSS and AMOS were used to analyze the data.FindingsML’s dimensions are positively linked to IWB. CSE was found as a mediator in these relationships. CS did not play its moderation roles on ML – CSE, ML – IWB direct or ML – IWB indirect (via CSE) links. ML’s direction-giving speech is found to be more effective in predicting CSE and IWB.Originality/valueThis is the first paper to examine the impacts of the three dimensions of ML on IWB.
Higher education leadership and context: a study of university vice-chancellors and presidents
Authors: Heffernan Troy , Eacott Scott , Bosetti Lynn 
Published: May 2021

PurposeUniversities claim to provide many benefits to their context. What remains less clear is what is meant by context. Whatever it is, context is fundamental to decision-making. Understanding what context means is crucial to understanding leadership in higher education.Design/methodology/approachTheoretically informed by Eacott's relational approach, this study is based on interview data from a purposive sample of ten English vice-chancellors and nine Canadian university presidents. Transcripts were analysed for the assumptions participants held regarding the work of universities and how that played out in practice.FindingsContext is not an external variable engaged with or acted upon. It is not separate to leadership and the work of universities but is constitutive of and emergent from activities. There is no single definition of context, and this has major implications for university activities.Research limitations/implicationsContext(s) is based on assumptions. Making explicit the assumptions of participants, without pre-defining them, is a key task of research on leadership in higher education.Practical implicationsLeaders need to explicitly articulate their assumptions regarding the work of universities. Assessment should be based on the coherence between the espoused position and activities undertaken.Originality/valueThrough the emerging resources of relational scholarship, this paper demonstrates how context is constitutive of and emergent from the activities of universities. More than novel vocabulary, the paper makes a fundamental point about the generative nature of context. De-centring entities (e.g. university, leader, context) and focusing on relations our approach provide a path forward by encouraging the articulation of intended purpose(s) and perspective on the work of universities.
Free flu shots vs herd immunity in flu vaccination advertising: the interaction of attribute type and message sidedness on flu vaccination judgment
Authors: Kim Kenneth Eunhan 
Published: May 2021

Purpose
This study aims to examine how the relative importance of a search versus a credence attribute, strategically addressed in a flu vaccination advertisement, varies as a function of message sidedness. A search attribute was designed to highlight the affordability of flu shots, and a credence attribute addressed the potential health benefits of flu vaccination.

Design/methodology/approach
Two experiments were designed to explore how the relative persuasiveness of search versus credence attributes varies as a function of message sidedness in the context of flu vaccination advertising. In Experiment 1, the search–credence attribute type was manipulated by addressing either the affordability (e.g. “Get free flu shots”) or indirect health benefits of flu vaccines (e.g. “Improve herd immunity/community health”). In Experiment 2, an individual-level credence attribute (e.g. “Strengthen your immune system”) was created and compared to the other two attribute conditions used in Experiment 1: a search versus a societal credence versus an individual credence attribute.

Findings
Experiment 1 (N = 114) revealed the relative advantage of a search attribute (free flu shots) in the two-sided persuasion. Experiment 2 (N = 193) indicated that the persuasive impact of a societal credence attribute (herd immunity/community health) was greater in the two-sided message condition (vs one-sided message condition).

Originality/value
Relatively little research has examined how consumers respond to strategic flu prevention and vaccination messages promoting either credence or search attributes. Motivated by the need to investigate the relative effectiveness of stressing “herd immunity” versus “free flu shots” in flu vaccination advertising, this study examines how the effects of these distinct attributes on flu vaccination judgments differ between two-sided (e.g. “No vaccine is 100% effective”) and one-sided persuasion.
Sports event portfolios: an innovative tool and a new management paradigm
Authors: Salgado-Barandela Jesyca , Barajas Angel , Sanchez-Fernandez Patricio 
Published: May 2021

PurposeThe organization of several small-scale events throughout the year is called event portfolio and may contribute to the development of the city strategy in the touristic, social and economic fields. The organization of a set of sporting events represents an important innovation in the development of city marketing. This paper analyzing the scarce literature on event portfolio provides the main elements to benefit from this innovative strategy to achieve sustainable sports tourism for host cities. Among the innovative strategies, the inclusion of leverage and cross-leverage approaches in the management of event portfolios is studied in depth.Design/methodology/approachThe study is divided into two phases. First, the Web of Science (WOS) and SCOPUS databases are searched using keywords. Once the selection of scientific studies that analyze the phenomenon of event portfolios has been obtained, an exhaustive review is carried out, allowing us to obtain novel findings on the field of study.FindingsThe work provides a classification of the portfolios of sports events analyzed in science considering the configuration (formal vs informal) and whether there is an integrated strategy. Second, the delimitation of the deficiencies and the most important aspects to be developed in sports-events portfolio management.Originality/valueThis study contributes with a summary of the main elements to consider when managing sports events portfolios. Moreover, the importance of integrated strategies is underlined, as well as the relevance of leveraging the strategies. The contributions are of interest in being a novel field with room for scientific development and with an eminently practical nature.
Technology intervention for preventing COVID-19 outbreak
Authors: Pandey Prateek , Litoriya Ratnesh 
Published: May 2021

PurposeThe purpose for writing this article is derived from the misery and chaos prevalent in the world due to the coronavirus pandemic – since late 2019 and still continuing as of December 2020.Design/methodology/approachA blockchain-based solution to verify the country visit trail and disease and treatment history of the passengers who arrive at the immigration counters located at various national borders and entry points is proposed. A fuzzy inference based suspect identifier system is also presented in this article that could be utilized to make further decisions based on the degree of suspicion observed on a particular passenger.FindingsThis paper attempted to put forth a blockchain-based system which consumes the healthcare and visit trail summary of a passenger (appearing for an interview before an immigration officer) and forwards it to a fuzzy inference system to reach to a conclusion that the passenger should be advised to self-quarantine, detained, or should be allowed to enter. Such a system would help to make correct decisions at the immigration counters to check pandemic diseases, like COVID-19, right at the entry points.Research limitations/implicationsThe implications of this work are manifold. First, the proposed framework works independent of the type of pandemic and is a readymade tool to check the spread of disease through infected human carriers. Second, the proposed framework will keep the mortality rates under check, which would give ample time for the authorities to save the lives of the people with co-morbidities and age vulnerabilities (Vichitvanichphonget al., 2018). Third, it is a general phenomenon to restrict the flights from the country where the first few cases of infection are discovered; however, the infected person, at the same time, might travel through alternative routes. The blockchain-enabled proposed framework ensures the detection of such cases at no other cost. Finally, the solution may appear costly in the first place, but it has the potential to hold back the revenue of the countries that would otherwise be spent on reactive measures.Originality/valueAs of now no other study or research article provides the solution to the biggest problem persists in the world in this way. The contribution is original and worth applying.
Modeling pervasive platforms and digital services for smart urban transformation using an enterprise architecture framework
Authors: Anthony Jnr Bokolo , Abbas Petersen Sobah , Helfert Markus , Ahlers Dirk , Krogstie John 
Published: May 2021

PurposeIn smart cities pervasive systems are deployed by enterprises and stakeholders in municipalities to provide digital services to citizens. But cities are faced with the challenge of achieving system pluggability, mainly service integration due to numerous actors and systems needed for smart urban transformation. Hence, there is need to employ a comprehensive and holistic approach to help achieve service integration of pervasive platforms. Therefore, this study presents an Enterprise Architecture Framework (EAF) to support smart urban transformation.Design/methodology/approachIn this study the design science research methodology is adopted based on a multi-case studies of two organizations and data is collected using semi-structured interview from an organizations and municipality in Norway to validate how service integration can be achieved by the developed EAF to address pluggability challenges faced in urban environment.FindingsFindings suggest that the presented EAF provides the structure to manage changes and maintain urban transformation and aims to align the business with the underlying information systems from the perspective of the stakeholders. Additionally, findings from the case studies modelled in ArchiMate language depict how service integration of different pervasive platforms provide digital services for smart urban transformation.Research limitations/implicationsThis research only employed semi-structured interviews to validate service integration of digital platforms, other identified dimensions of pluggability were not fully addressed in this study.Practical implicationsFindings from the case studies provides insights on how pervasive platforms can be integrated to achieve a pluggable digital service from different stakeholders and data sources in practice. The developed EAF presented in this study provide a model that supports collection and exchange of data from different data sources in smart urban environment to enable the provision and consumption of digital services.Social implicationsThe developed EAF aids system pluggability of actors and systems in providing digital service such as smart urban transformation that contributes to sustainable use of electric mobility in cities.Originality/valueAs cities increasingly deploy pervasive platforms to support urban innovation, researchers are seeking to explore how these platforms shape urban transformation. Presently, prior studies do not offer important insights into pervasive platform management from urban perspective. Against this backdrop, this study employs the information systems perspective of digital platforms literature roots in software development and physical product development to depict how the EAF can be employed to describe specific cases that integrate different pervasive platforms deployed by different stakeholders communicating to co-create collective digital services to citizens.
Challenges of agri-food supply in city region food systems: an emerging economy perspective
Authors: Weerabahu Samanthi Kumari , Samaranayake Premaratne , Dasanayaka S.W. Sarath , Wickramasinghe Chaminda Nalaka 
Published: May 2021

PurposeThis paper explores the challenges of food security from source to consumption of agri-food value chain by considering urban–rural linkages in city region food systems (CRFSs) and proposes a strategic framework for CRFS identifying strategies to promote urban–rural linkages among multiple stakeholders.Design/methodology/approachA qualitative case study approach to a fruit and vegetable value chain from rural source to consumption in the Colombo City region identifies the challenges of food security. A snowballing sampling method was used to gather information from retailers, wholesalers, commission agent, farmers and consumers. The data were collected through face-to-face interviews, observations and secondary data sources. The data were analysed using thematic analysis.FindingsChallenges in food security in the value chain related to five areas: input and production, infrastructure, public institutional support and policy, finance, and food market. Colombo city is heavily dependent on food sourced from other cities due to limited land and lack of locally situated commercially oriented farmers.Research limitations/implicationsThis research is limited to a selected number of fruits and vegetables in the Colombo city region and leaves out other food items.Originality/valueThis study contributes to informing policy and decision-making processes to promote a more balanced rural to city food value chain in Colombo City that could benefit all stakeholders from rural small-scale producers to urban consumers.