bluuebunny/crossref_metadata_2025_split huggingface dataset:
1-kHz high-frequency spinal cord stimulation alleviates chronic refractory pain after spinal cord injury: a case report
Authors: Yamada Chiaki , Maeda Aiko , Matsushita Katsuyuki , Nakayama Shoko , Shirozu Kazuhiro , Yamaura Ken 
Published: June 2021

Abstract
Background
Patients with spinal cord injury (SCI) frequently complain of intractable pain that is resistant to conservative treatments. Here, we report the successful application of 1-kHz high-frequency spinal cord stimulation (SCS) in a patient with refractory neuropathic pain secondary to SCI.

Case presentation
A 69-year-old male diagnosed with SCI (C4 American Spinal Injury Association Impairment Scale A) presented with severe at-level bilateral upper extremity neuropathic pain. Temporary improvement in his symptoms with a nerve block implied peripheral component involvement. The patient received SCS, and though the tip of the leads could not reach the cervical vertebrae, a 1-kHz frequency stimulus relieved the intractable pain.

Conclusions
SCI-related symptoms may include peripheral components; SCS may have a considerable effect on intractable pain. Even when the SCS electrode lead cannot be positioned in the target area, 1-kHz high-frequency SCS may still produce positive effects.
Association of lipoproteins and thyroid hormones with cognitive dysfunction in patients with systemic lupus erythematosus
Authors: Lu Li , Kong Wei , Zhou Kangxing , Chen Jinglei , Hou Yayi , Dou Huan , Liang Jun 
Published: June 2021

Abstract
Background
Neuropsychiatric manifestations occur in up to 75% of adult systemic lupus erythematosus (SLE) patients and are one of the major causes of death in SLE patients. Cognitive dysfunction is a typical clinical feature of neuropsychiatric SLE (NPSLE), which seriously affects the quality of life of patients. Dyslipidaemia and thyroid symptoms, which are prevalent in SLE patients, have both been related to neuropsychiatric disturbances, including significant psychiatric and cognitive disturbances. This study aimed to investigate whether cognitive dysfunction in patients with SLE was related to the expression of serum thyroid hormone and lipoprotein levels.

Methods
A total of 121 patients with SLE and 65 healthy controls (HCs) at Nanjing Drum Tower Hospital completed a cognitive function test, and 81 SLE patients were divided into a high-cognition (n = 33) group and a low-cognition group (n = 48). The clinical and laboratory characteristics of the patients were compared; moreover, correlations between serum HDL-C, LDL-C, F-T3 and F-T4 levels and cognitive function were analysed. Serum levels of APOE, APOA1, IGF-1, and IGFBP7 in 81 patients were detected by ELISA, and the correlation between these four proteins and cognition was analysed separately.

Results
The patients with SLE with abnormal cognitive function were less educated than the HCs. For low-cognition patients, the levels of albumin, F-T3 (P <  0.05) and F-T4 decreased, while D-dimer, anti-dsDNA antibody, and IgM levels increased. Serum F-T3 and F-T4 levels positively correlated with cognition. Furthermore, serum protein levels of APOE and APOA1 showed no difference between the high- and low-cognition groups. However, the serum APOE levels were negatively correlated with line orientation scores, and APOA1 levels were positively correlated with coding scores.

Conclusions
Serum F-T3 and F-T4 levels were both positively correlated with four indexes of cognition (language was the exception), while serum APOE levels were negatively correlated with line orientation scores, APOA1 levels were positively correlated with coding scores, and IGFBP7 levels were negatively correlated with figure copy scores. These results demonstrated that F-T3 and F-T4 might be clinical biomarkers of cognitive dysfunction in SLE.
The consequence of magnetic field on the parameters of brackish water in batch and continuous flow system
Authors: El-Shamy Ashraf M. , Abdo Ahmed , Gad Elshafie A. M. , Gado Amr A. , El-Kashef Emad 
Published: June 2021

AbstractBackgroundThe concept of magnetized water and the historically abbreviated glimpse were discussed. Therefore, the magnetic water treatment method has been summed up and considered a better and cleaner physical technique for water handling. This experimental work is focused on the effect of magnetic treatment on certain water parameters such as temperature, electrical conductivity (EC), total dissolved salts (TDS), and pH by exposing water to a permanent magnetic field (PMF) with a magnetic flux density (B = 1.45 T ± 0.05).ResultsThis technique is realized by using a fixed system that depends on the application of both pump and valve control to induce the required circulation of employed water. Both open loop and closed loop are applied as a function of exposure time. Considering that the type of used water is brackish groundwater. The results showed that at open and closed flow conditions, the PMF causes variations in the values of the measured parameters for the outflow water. The theoretical approach is subjected to measure the molecular interaction of water system H-bonded systems based on DFT level with function B3LYP on Gaussian 09 software with a specific concentration of NaCl. This research focuses on the relation between the molecular structure of water and the dissolved NaCl with respect to applying a magnetic field with a varying force from 1 to 85 T.ConclusionThe water's magnetization technique is simple without using extra energy by using a PWT tool to create a permanent magnetic field (B = 1.45 T ± 0.05) when installing it on a water tube system that was previously mounted. This environmentally friendly, renewable technology, therefore, does not need any additional energy requirements.
Patterns of central nervous system complications of post-hematopoietic stem cell transplant in pediatric oncology patients: a single institute experience
Authors: Hussein Samar Ahmed , Hammad Mahmoud , Abdalla Amr , Alsheshtawi Khaled , Zaky Iman Mohamed , Youssef Ayda 
Published: June 2021

Abstract
Background
Hematopoietic stem cell transplant (HSCT) has been increasingly used in the last few decades, with improved success in offering a cure. CNS complications are an important contributor to morbidity and mortality in HSCT patients. The aim of the study was to evaluate the role of imaging in the detection and assessment of CNSC (central nervous system complications) after HSCT in pediatric oncology patients.
The study included consecutive pediatric patients who underwent HSCT for hematologic or solid malignancies at CCHE-57357 (Children Cancer Hospital–Egypt 57357) from January 2011 to March 2019. The age of the patients in the study ranged from 0.9 to 25 years (median age 6.5 years). CT (computed tomography) and/or MRI (magnetic resonance imaging) studies were evaluated for the detection and characterization of CNSC.

Results
The incidence of post-HSCT CNSC was 13% with a day 100 and 5-year cumulative incidence of 9.3 and 12.5%, respectively. The most commonly observed CNSC detected was disease recurrence, followed by PRES (Posterior reversible encephalopathy syndrome). CNS recurrence of the initial diagnosis, atrophy, and infection were more common at the > 100-day post-HSCT transplant period, while PRES was much more common at < 100-day post-HSCT.

Conclusion
CNS complications are an important contributor to morbidity and mortality in HSCT patients that require MRI protocols distinctively tailored for each patient, clinical suspicion, and proper imaging assessment for early detection and follow-up.
Managing returns to prison from medium-secure services: qualitative study
Authors: Leonard Sarah-Jayne , Sanders Caroline , Shaw Jennifer J. 
Published: June 2021

Background
Little is known about factors that influence discharge decision-making for people admitted to medium-secure services from prison, particularly for those who are returned to prison following treatment.

Aims
To explore the organisational influences on care pathways through medium-secure services for those admitted from prison.

Method
We recruited 24 clinicians via purposive and snowball sampling; 13 shared their experiences via a focus group, and 11 shared their experiences via individual semi-structured interviews. A thematic analysis was conducted, producing three overarching themes: maintenance of throughput and service provision, class of two systems, and desirable and undesirable patients.

Results
Data indicated external factors that direct and, at times, limit clinicians’ pathway decisions, including commissioning criteria and legal status under the Mental Health Act 1983 and within the criminal courts system (i.e. whether on remand or sentenced). These factors also influence how clinicians view the role and function of medium-secure services within the wider forensic mental health system, and therefore the types of patients that are deemed ‘appropriate’ for continued treatment when making discretionary pathway decisions.

Conclusions
There remains a deficit in adequate resources to meet the mental health needs of prisoners who are admitted to medium-secure services. To meet the clinical need of all admissions, criteria for prolonged treatment in medium-secure services needs to be reconsidered, and it is likely that provision for the medium-secure hospital estate will need to increase substantially if effective rehabilitation of those who transfer from prison is to take place.
Comparison of Serum and Urinary 5-Hydroxyindoleacetic Acid as Biomarker for Neuroendocrine Neoplasms
Authors: Becker Anna , Schalin-Jäntti Camilla , Itkonen Outi 
Published: June 2021

Abstract

Context
Patients with serotonin-secreting neuroendocrine neoplasms (NENs) have increased serum 5-hydroxyindoleacetic acid (5HIAA) concentrations. Serum 5HIAA thus serves as a biomarker in NEN.

Objective
To evaluate an improved tandem mass spectrometric serum 5HIAA assay for diagnosis and follow-up of NEN in a clinical cohort.

Design
A retrospective study during 2016-2018 at the Diagnostic Center and Department of Endocrinology at Helsinki University Hospital, Finland.

Methods
Detailed patient data was obtained from 116 patients. Serum 5HIAA was analyzed by 2 different liquid chromatography with tandem mass spectrometry (LC-MS/MS) assays with samples prepared either by protein precipitation or solid phase extraction. Twenty-four–hour urine 5HIAA samples (n = 33) were analyzed by amperometric LC, and the results were compared. Specificity and sensitivity were calculated by receiver operating characteristic (ROC) analysis.

Results
We achieved 5 to10 000 nmol/L linearity and ≤2.5% variation with our new serum 5HIAA assay. In ROC analysis, the area under curve was 85% by serum assays [upper reference limit (URL) value 123 nmol/L] and 88% by the 24-h urine 5HIAA assay (URL value of 47.1 µmol), respectively. A difference (P &lt; 0.001) between patients with active NEN and patients in remission was found by all 5HIAA assays.

Conclusion
Serum 5HIAA by LC-MS/MS after protein precipitation performs equally well for the diagnosis of NEN as urinary 5HIAA LC assay. The outcome and sensitivity for serum and 24-h urine assays are convergent. Due to much more reliable and convenient sampling, we recommend serum instead of 24-h urine 5HIAA for diagnosis and follow-up of NEN patients.
MICAL-L1 is required for cargo protein delivery to the cell surface
Authors: Sikora R. , Bun P. , Danglot L. , Alqabandi M. , Bassereau P. , Niedergang F. , Galli T. , Zahraoui A. 
Published: June 2021

ABSTRACT
Secreted proteins are transported along intracellular route from the endoplasmic reticulum through the Golgi before reaching the plasma membrane. Small GTPase Rab and their effectors play a key role in membrane trafficking. Using confocal microscopy, we showed that MICAL-L1 was associated with tubulo-vesicular structures and exhibited a significant colocalization with markers of the Golgi apparatus and recycling endosomes. Super resolution STORM microscopy suggested at the molecular level, a very close association of MICAL-L1 and microdomains in the Golgi cisternae. Using a synchronized secretion assay, we report that the shRNA-mediated depletion of MICAL-L1 impaired the delivery of a subset of cargo proteins to the cell surface. The process of membrane tubulation was monitored in vitro, and we observe that recombinant MICAL-L1-RBD domain may contribute to promote PACSINs-mediated membrane tubulation. Interestingly, two hydrophobic residues at the C-terminus of MICAL-L1 appeared to be important for phosphatidic acid binding, and for association with membrane tubules. Our results reveal a new role for MICAL-L1 in cargo delivery to the plasma membrane.
Universal principles of lineage architecture and stem cell identity in renewing tissues
Authors: Greulich Philip , MacArthur Ben D. , Parigini Cristina , Sánchez-García Rubén J. 
Published: June 2021

ABSTRACT
Adult tissues in multicellular organisms typically contain a variety of stem, progenitor and differentiated cell types arranged in a lineage hierarchy that regulates healthy tissue turnover. Lineage hierarchies in disparate tissues often exhibit common features, yet the general principles regulating their architecture are not known. Here, we provide a formal framework for understanding the relationship between cell molecular ‘states’ and cell ‘types’, based on the topology of admissible cell state trajectories. We show that a self-renewing cell type – if defined as suggested by this framework – must reside at the top of any homeostatic renewing lineage hierarchy, and only there. This architecture arises as a natural consequence of homeostasis, and indeed is the only possible way that lineage architectures can be constructed to support homeostasis in renewing tissues. Furthermore, under suitable feedback regulation, for example from the stem cell niche, we show that the property of ‘stemness’ is entirely determined by the cell environment, in accordance with the notion that stem cell identities are contextual and not determined by hard-wired, cell-intrinsic characteristics.
This article has an associated ‘The people behind the papers’ interview.
The evolutionary biomechanics of locomotor function in giant land animals
Authors: Hutchinson John R. 
Published: June 2021

ABSTRACTGiant land vertebrates have evolved more than 30 times, notably in dinosaurs and mammals. The evolutionary and biomechanical perspectives considered here unify data from extant and extinct species, assessing current theory regarding how the locomotor biomechanics of giants has evolved. In terrestrial tetrapods, isometric and allometric scaling patterns of bones are evident throughout evolutionary history, reflecting general trends and lineage-specific divergences as animals evolve giant size. Added to data on the scaling of other supportive tissues and neuromuscular control, these patterns illuminate how lineages of giant tetrapods each evolved into robust forms adapted to the constraints of gigantism, but with some morphological variation. Insights from scaling of the leverage of limbs and trends in maximal speed reinforce the idea that, beyond 100–300 kg of body mass, tetrapods reduce their locomotor abilities, and eventually may lose entire behaviours such as galloping or even running. Compared with prehistory, extant megafaunas are depauperate in diversity and morphological disparity; therefore, turning to the fossil record can tell us more about the evolutionary biomechanics of giant tetrapods. Interspecific variation and uncertainty about unknown aspects of form and function in living and extinct taxa still render it impossible to use first principles of theoretical biomechanics to tightly bound the limits of gigantism. Yet sauropod dinosaurs demonstrate that &gt;50 tonne masses repeatedly evolved, with body plans quite different from those of mammalian giants. Considering the largest bipedal dinosaurs, and the disparity in locomotor function of modern megafauna, this shows that even in terrestrial giants there is flexibility allowing divergent locomotor specialisations.
Extracorporeal Shockwave Therapy in the Management of Sports Medicine Injuries
Authors: Schroeder Allison N. , Tenforde Adam S. , Jelsing Elena J. 
Published: June 2021

Abstract
Treatment of musculoskeletal conditions in athletes with extracorporeal shockwave therapy (ESWT) is gaining popularity as greater evidence supports its use. ESWT protocols (describing energy flux density, number of impulses, type of shockwave (focused or radial), number/frequency/duration of treatment session, area of application, and postprocedural therapy protocols) can be adjusted in the clinical setting. Protocols vary across studies, and optimal protocols for most indications are yet to be determined. ESWT can safely be used to treat various musculoskeletal conditions in athletes, including rotator cuff tendinopathy, lateral elbow epicondlyopathy, greater trochanteric pain syndrome, hamstring tendinopathy, patellar tendinopathy, Achilles tendinopathy, other tendinopathies, plantar fasciopathy, bone stress injuries, and medial tibial stress syndrome. ESWT can be used to treat in-season athletes, as it often requires no/minimal time away from sport and may result in rapid benefits. ESWT should be used in conjunction with physical therapy to facilitate longer-term gains in function and to optimize healing.
The Epidemiology, Risk Factors, and Nonsurgical Treatment of Injuries Related to Endurance Running
Authors: Raghunandan Aditya , Charnoff Jesse N. , Matsuwaka Sean T. 
Published: June 2021

Abstract
Running is a popular form of exercise that is easily accessible to various populations; endurance running, defined as distances beyond 5 km, continues to grow within the sport. Endurance running-related injuries are common in the lower extremities and are primarily overuse related. A multitude of risk factors for injury exist, including extrinsic factors, such as running distance and frequency, and intrinsic factors, such as biomechanics and nutrition status. Training and rehabilitation techniques vary with a general focus on strengthening and gradual increase in activity, but evidence is mixed, and it is difficult to generalize programs across different running populations. Management of specific running groups, including youth runners, is an area in which additional research is needed. New treatments, such as orthobiologics and wearable technology, have promising potential to optimize performance and recovery and minimize injury. However, they need to be further evaluated with high-quality studies.
Development of a checklist to detect errors in meta-analyses in systematic reviews of interventions: study protocol
Authors: Kanukula Raju , Page Matthew , Dwan Kerry , Turner Simon , Loder Elizabeth , Mayo-Wilson Evan , Li Tianjing , Misra Adya , McDonald Steve , Forbes Andrew , McKenzie Joanne 
Published: June 2021

Background: Systematic reviews underpin clinical practice and policies that guide healthcare decisions. A core component of many systematic reviews is meta-analysis, which is a statistical synthesis of results across studies. Errors in the conduct and interpretation of meta-analysis can lead to incorrect conclusions regarding the benefits and harms of interventions; and studies have shown that these errors are common. Enabling peer reviewers to better detect errors in meta-analysis through the use of a checklist provides an opportunity for these errors to be rectified before publication. To our knowledge, no such checklist exists. Objective: To develop and evaluate a checklist to detect errors in pairwise meta-analyses in systematic reviews of interventions. Methods: We will undertake a four-step process to develop the checklist. First, we will undertake a systematic review of studies that have evaluated errors in the conduct and interpretation of meta-analysis to generate a bank of items to consider for the checklist. Second, we will undertake a survey of systematic review methodologists and statisticians to seek their views on which items, of the bank of items generated in step 1, are most important to include in the checklist. Third, we will hold a virtual meeting to agree upon which items to include in the checklist. Fourth, before finalising the checklist, we will pilot with editors and peer reviewers of journals. Conclusion: The developed checklist is intended to help journal editors and peer reviewers identify errors in the application and interpretation of meta-analyses in systematic reviews. Fewer errors in the conduct and improved interpretation will lead to more accurate review findings and conclusions to inform clinical practice.
Immunotropic effects of nitrogenous metabolites (creatinine, urea, uric acid and bilirubin) in humans exposed to the factors of the accident at the Chоrnobyl nuclear power plant
Authors: Kuchma Igor , Gozhenko Anatoliy , Bilas Volodymyra , Ruzhylo Sofiya , Kovalchuk Galyna , Nahurna Yaryna , Zukow Walery , Popovych Igor 
Published: June 2021

Background. In previous studies, we found that nitrogenous metabolites exhibit significant immunotropic activity, both suppressor and enhancing, at healthy rats. The purpose of this and our subsequent research is to elucidate the links between nitrogenous metabolites and immune parameters in different categories of people, both healthy and sick. Material and methods. The object of observation in 1997 were 19 men and 3 women who were exposed to pathogenic factors of the accident at the Chоrnobyl nuclear power plant during the liquidation of its consequences in 1986-87. The survey was conducted twice - on admission and after two weeks of rehabilitation at the Truskavets’ Spa. The plasma and urinary concentration of the nitrogenous metabolites were determined. Immune status was assessed on tests of I and II levels according to the WHO memorandum. Results. Both negative and positive metabolic-immune correlations were revealed. Calculation of multiple correlation coefficients between individual metabolite parameters and constellations of immune parameters revealed the maximum immunotropic effect of Urea urine (R=0,756). This is followed by Uric acid plasma (R=0,727) and urine (R=0,691), Urea plasma (R=0,622), Creatinine plasma (R=0,588), Bilirubinemia (R=0,546) and Creatinine urine (R=0,510). The canonical correlation between the constellation of nitrogenous metabolites, on the one hand, and the parameters of immunity, on the other hand, was very strong: R=0,971; χ2(15)=239; p<10-5. Conclusion. Nitrogenous metabolites have immunomodulatory effects, both suppressor and enhancing, both in healthy rats and in humans exposed to pathogenic influences.