bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effect of Health Education Via Mobile Application in Promoting Quality of Life Among Asthmatic Schoolchildren in Urban Malaysia: A Quasi-Experimental Study
Authors: Raimi Abdulaziz Mansoor Al , Chong Mei Chan , Tang Li Yoong , Chua Yan Piaw , Ajeel Latifa Yahya Al 
Published: June 2021

Abstract
BACKGROUND: Bronchial asthma among children is a common chronic disease which may have impact on quality of life. Health education is one of the strategy to improve knowledge and quality of life.OBJECTIVE: The study aims to assess the effect of health education via mobile application (app) in promoting quality of life among schoolchildren with asthma in urban Malaysia.METHODS: A quasi-experimental, pre- and post-intervention design was used in this study during the period September 2016 until April 2017 involving a total of 214 students, randomly assigned into two groups (intervention group and control group). The control group received face-to-face health education while the experimental group received health education via a mobile application.RESULTS: The findings showed that the total score of quality of life (QoL) has improved from a mean total score at pre-intervention (5.31±1.27) to post-intervention (5.66±1.28) for the control group, compared with the experimental group with a mean total score of QoL at pre-intervention (5.01±1.36) and post-intervention (5.85±1.29). A comparison between the experimental and control groups using an independent t-test showed statistically significant differences in their mean QoL scores. The effect of health education via mobile application showed a statistically significant improvement in the mean QoL score from pre- to post-intervention [F (1,288) = 57.46, p = <0.01].CONCLUSION: The use of mobile technology in health education improved QoL as compared to the traditional methods of face-to-face lecture and/or handbooks among schoolchildren with asthma. Thus, educational modules using mobile applications do improve QoL.
Structural Insights Into The Binding of Nanobodies LaM2 and LaM4 to the Red Fluorescent Protein mCherry
Authors: Wang Ziying , Li Long , Hu Rongting , Zhong Peiyu , Zhang Yiran , Cheng Shihao , Liu Rui , Ding Yu 
Published: June 2021

Abstract
Red fluorescent proteins (RFPs) are widely used in molecular biology research, especially in deep tissues and animal models, because of their superior autofluorescence, light scattering, and phototoxicity to GFP. Although RFP can be easily monitored in vivo, improved manipulation of RFP is still desired. Using suitable nanobodies (Nbs) to bind to different epitopes of RFP is the most promising approach; thus, it is crucial to obtain structural information on how the different Nbs interact with RFP. We determined the crystal structures of the LaM2-mCherry and LaM4-mCherry complexes at 1.4 Å and 1.9 Å resolution. Our results showed that LaM2 binds to the side of the mCherry β-barrel, while Lam4 binds to the bottom of the β-barrel and does not interfere with the homo-oligomerization interface. The distinct binding sites of LaM2 and LaM4 were further verified by ITC, F-SEC and DLS assays. Our results also showed that LaM2 and LaM4 can bind simultaneously to mCherry, which is crucial for recruiting multiple operation elements to the RFP. The binding of LaM2 or LaM4 did not significantly change the chromophore environment of mCherry, which is important for fluorescence quantification assays, while several GFP Nbs significantly altered the fluorescence. Mutation of the residues of the LaM2 or LaM4 binding interface to mCherry significantly decreased the binding affinity of the Nb to mCherry. Our results provided atomic resolution interaction information on the binding of Nbs LaM2 and LaM4 binding with mCherry, which is important for developing detection and manipulation methods for RFP-based biotechnology.
ADAM12 as a Clinical Prognostic Indicator Associated with Tumour Immune Infiltration in Lung Adenocarcinoma
Authors: Pan Junfan , Huang Zhidong , Xu Yiquan 
Published: June 2021

Abstract
Background: Lung cancer is the most common cause of cancer-related death worldwide. In humans, 22 functional α-disintegrin and metalloproteinases (ADAMs) have been identified, 12 of which have proteolytic activity. The role of ADAMs in cancer has attracted increasing attention. However, the expression and significance of ADAMs in lung adenocarcinoma (LUAD) remain unclear. The current study aimed to explore the expression and prognostic value of ADAM12in LUAD.Methods: The Cancer Genome Atlas(TCGA) database was used to analyse the expression of ADAMs in LUAD. The cBioPortal database was used to obtain and analyse ADAM12 copy number changes, and the LinkedOmics database was utilised for the analysis of ADAM12-related genes. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis was then performed using TIMER. The relationship between ADAM12 and the tumour immune microenvironment(TIM) was assessed via the TISIDB database. ADAM12 and immune-related genes were used to construct a prognostic model. Knockdown of ADAM12 was performed in vitro in order to verify its biological function. The unpaired t-test was used for comparison between the two groups, ANOVA was used for analysing differences between multiple groups, and the Kaplan-Meier test was used to compare survival between groups.Results: Most ADAMs exhibited significant differential expression in LUAD. ADAM12 expression was significantly higher in LUAD tissues than in healthy tissues, and lower ADAM12 expression was associated with better survival. Genetic alterations of ADAM12 mainly included missense mutations, amplifications, and deep deletions. ADAM12 and positively correlated genes were mainly enriched in protein digestion and absorption, ECM-receptor interaction, and adhesion plaques. ADAM12 had a moderate correlation with immune cell markers EBIP1, CCNB1, EXO1, KNTC1, PRC1 and FAM198B. Prognostic model was established based on ADAM12 and immune-related genes. In vitro experiments revealed that knocking down ADAM12 inhibited cell proliferation, migration and invasion.Conclusion: ADAM12 potentially plays an important role in the occurrence of LUAD and may be utilised as an immunotherapy target and a valuable prognostic biomarker for LUAD.
Diagnostic accuracy and prognostic relevance of immunoglobulin heavy chain rearrangement and 18 F-FDG- PET/CT compared with unilateral bone marrow trephination for detecting bone marrow involvement in patients with diffuse large B-cell lymphoma
Authors: Kim Mihee , Ahn Seo-Yeon , Ahn Jae-Sook , Song Ga-Young , Jung Sung-Hoon , Lee Je-Jung , Kim Hyeoung-Joon , Lee Jun Hyung , Shin Myung-Geun , Song Sang Yun , Yang Deok-Hwan 
Published: June 2021

Abstract
Background

In diffuse large B-cell lymphoma (DLBCL), bone marrow involvement (BMI) has an important clinical implication as a component of staging and International Prognostic Index (IPI).
Methods

This study aimed to determine whether molecular analysis of immunoglobulin heavy chain (IgH) genes and PET/CT could overcome the limitation of defining morphologic bone marrow involvement by trephination biopsy and could increase the diagnostic accuracy or prognostic prediction. 94 de novo patients with DLBCL underwent PET/CT, polymerase chain reaction (PCR) test for detection of IgH gene rearrangement, and unilateral BM trephination at diagnosis.
Results

9 patients (9.6%) were confirmed to present morphologic BMI (mBMI) based on trephination biopsy. On the other hands, 21 patients (22.3%) were confirmed to have IgH clonality (IgH BMI), while 16 (17.0%) were classified with BMI based on the assessment of PET/CT (PET BMI). Each IgH rearrangement PCR and PET/CT showed the high negative predict value of detecting the BMI. However, the combined assessment of IgH rearrangement and PET/CT could increase a diagnostic accuracy and specificity with 87.2% and 97.0%, respectively. The survival outcome of patients with double positive PET BMI and IgH BMI was significantly worse than that with either single positive PET BMI or IgH BMI, and even less than patients with neither PET BMI nor IgH BMI. (3-year PFS: 50.0% vs 75.4% vs 97.9%, p = 0.007, 3-year OS: 50.0% vs 75.6% vs 80.1%, p = 0.035, respectively).
Conclusion

This study suggested that the combined evaluation of PET/CT and IgH rearrangement could give additional information of predicting therapeutic outcomes in patients with negative morphologic BMI as an important part of the prognosis.
Providing Information Improved Adequate Storage and Disposal of Opioids by Bereaved Family Caregivers
Authors: Okizaki Ayumi , Miura Tomofumi , Fujishiro Noriko , Igarashi Naoko , Matsumoto Yoshihisa , Naito Akemi Shirado , Shima Yasuo , Kinoshita Hiroya , Reddy Akhila , Morita Tatsuya , Miyashita Mitsunori 
Published: June 2021

Abstract
Context:

Poor understanding regarding the safe use, secure storage, and adequate disposal of unused opioids may contribute to the opioid epidemic. It is unclear what happens to unused opioids in the households of deceased cancer patients.
Objectives:

Our aim was to investigate the patterns of use, storage, and disposal of unused opioids among families of deceased cancer patients.
Methods:

A questionnaire was administered to families of deceased cancer patients. Questions were related to the use, storage, and disposal of opioids prescribed to their deceased family member.
Results:

Completed questionnaires were received from the families of 240 patients. Of these, 129 (53.8%) and 99 (41.3%) participants were aware of the danger of diversion and need for safe disposal, respectively. One (0.4%) participant reported diversion. In total, 177 participants (73.8%) stored opioids securely, 6 (2.5%) had unused opioids at home, and 142 (59.2%) returned unused opioids to medical health providers. Knowledge of safe opioid disposal increased the rate of returning opioids to medical health providers (84.8% vs. 53.2%; p < 0.001) and decreased the rate of participant disposal (13.3% vs. 37.6%; p < 0.001). Prior knowledge of the risks of opioid diversion was associated with storing opioids securely (91.9% vs. 80.7%; p = 0.028).
Conclusion:

Most families of deceased cancer patients follow safe use, secure storage, and adequate disposal of prescribed opioids. Awareness of opioid diversion and the need for adequate disposal increased the prevalence of safe practices.
The Reverberation of Corona Pandemic in Developing Countries Special Reference To Ethiopia
Authors: Ayalew Abebe Temesgen , Aynalem Elsabet Getachew 
Published: June 2021

Abstract
Purpose - The present COVID 19 virus has unexpected social problem with an overwhelming financial influence happening in each family circle. This research aimed to assess the overall influence of COVID 19 in selected cities in Ethiopia which has high population density, commercial, high local population movement and tourist attractive towns.Design/Methodology/Approach - For a random sampling illustration 50,000 defendants are assessed through virtual mode commencing 17 cities of Ethiopia. For verifying the demonstrative of the statistics, demographic characters from online survey non behalf of time-based variations for each sample size. While survey in each city inadequate information collection is not bounded, studies for defendant’s IP address not correspond to the nation; studies labeled as redundant, assessments with illegal replies, and studies irrational accomplishment period for each sample.Findings - From this research one can understand the pecuniary influences increasing in alarming rate and incapable: out of the total respondents 45 percent of a family member missing their job and 59 percent of defendants report that a household member has closed their business. The rest households owning small businesses and among families with the lowermost income aforementioned for the pandemic, 71 percent information shows that a family member missing their job and 61 percent report that a household member has stopover their private business. Deteriorations in food safety and healthiness are among the incapable influences.Originality/value – Virtual method of survey, random sampling and statistical regression analysis was better in signifying the COVID virus effect in different aspects
Epsilon Near Zero (ENZ) Based Kagome Photonic Crystal Fiber for Low Loss, High Birefringence and Near Zero Flat Dispersion in THz Regime
Authors: Asaduzzaman Sayed , Rehana Hasin , Aziz M. D. Tanzil , Faragallah Osama S. , Baz Mohammed , Eid Mahmoud M. A. , rashed ahmed nabuh zaki 
Published: July 2021

Abstract
In this research, we have proposed two novel types of Kagome PCFs one is slotted core PCF (S-KPCF) and another one is circular PCF (C-KPCF). FEM has been applied with boundary condition to investigate the optical properties of Proposed PCFs. HRS material along with Epsilon Near Zero (ENZ) material has been applied in core and TOPAS was used as background material. Numerical investigation takes place a wider frequency range for 0.8 THz to 2 THz. Slotted core PCF (S-KPCF) shows eminent birefringence of 2.98×10− 2, Lower EML of 0.232 cm− 1, lower confinement loss of 2.76×10− 8, Bending loss 10.76×10− 9, Total loss of 0.232cm− 1 at frequency 1THz. Besides circular core PCF (C-KPCF) shows eminent birefringence of 8.69×10− 2, Lower effective material loss of 0.746 cm− 1, lower confinement loss of 2.80×10− 7, Bending loss 7.76×10− 9, Total loss of 0.746cm− 1 at 1THz. Both PCFs show flat dispersion near zero (for S-KPCF is -0.997ps/nm/Km and for C-KPCF is -0.797ps/nm/Km) range. V-Parameter of both of the PCF indicates that both PCF is multimode PCF. Comparison of the proposed PCFs shows that C-KPCF shows better results than S-KPCF and both PCFs shows better results than previous PCFs.
Identification of Possible Inhibitors of SARS-CoV-2 Main Protease From Some Bioactive Compounds of Artemisia Annua: An in Silico Approach
Authors: Johnson Titilayo , Adegboyega Abayomi , Ojo Oluwafemi , Jega Amina , Iwaloye Opeyemi , Ugwah-Oguejiofor Chinenye , Asomadu Rita , Chukwuma Ifeoma , Ejembi Stephen Ejembi , Ugwuja Emmanuel 
Published: June 2021

Abstract
The inhibitory potential of Artemisia annua, a well-known antimalarial herb, against several viruses including the coronavirus is increasingly gaining recognition. The plant extract has shown significant activity against both the Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV) and the novel SARS-CoV-2, that is currently ravaging the world. It is therefore necessary to identify the bioactive compounds of the plants that are responsible for this activity, for the purpose of designing drugs against SARS-CoV-2. In this study, we employed in silico techniques comprising of molecular docking, binding free energy calculations, pharmacophore modelling as well as druglikeness, pharmacokinetics and toxicity predictions, to identify potential inhibitors of the SARS-CoV-2 main protease (Mpro) from 168 bioactive compounds of Artemisia annua. Rhamnocitrin, Isokaempferide, Kaempferol, Quercimeritrin, Apigenin, Penduletin, Isoquercitrin, Astragalin, Luteolin-7-glucoside and Isorhamnetin were ranked highest; with docking scores ranging from -7.84 to -7.15 kcal/mol compared with -6.59 kcal/mol demonstrated by the standard ligand. Rhamnocitrin, Isokaempferide and Kaempferol, like the standard ligand interacted with important active site amino acid residues like HIS 41, CYS 145, ASN 142, and GLU 166, among others. These compounds also possess acceptable druglike properties and safety profile. Hence, they could be considered for experimental studies and further development into drugs against SARS-CoV-2.
Patient-Reported Outcomes in a Chinese Cohort of Osteogenesis Imperfecta Unveil Psycho-Physical Stratifications Associated with Clinical Manifestations
Authors: Chen Peikai , Tan Zhijia , Zhou Yapeng , Yin Shijie , Dong Zhongxin , Qiu Yan , Xu Jichun , Li Kangsen , Qiu Anmei , Dong Lina , Shek Hiu Tung , Liu Jingwen , Yeung Eric Hiu Kwong , Gao Bo , Cheung Kenneth Man Chee , To Michael Kai-Tsun 
Published: June 2021

Abstract
Background: Osteogenesis imperfecta (OI) is a rare congenital disorder that greatly compromises the quality of bones, inflicting debilitating distress both physically and psychologically to patients and caregivers. Over the decades, much efforts have been channeled towards understanding genetic mechanisms and exploring new treatments. Nonetheless, it has recently become more aware that the patient reported outcomes (PRO) and health status during treatment, healing and rehabilitation, are important basis that facilitates smoother communication, refines interventions strategies and achieves higher quality of life. To date, systematic studies and analyses of PRO in OI patients remain scarce.Results: Here, harnessing the Patient-Reported Outcomes Measurement Information System (PROMIS), we report a cross-sectional and longitudinal study in a cohort of 90 OI patients from southern China, covering both children and adult age-groups. In the young group where both self and parental surveys were obtained, we identified two clusters of comparable sizes showing different outlooks in physical mobility and emotional experiences. One cluster is more positive about themselves than the other. Remarkably, we found a concordance of 84.7% between self and parental assessments, suggesting the stability of the stratification and validity of the PROMIS instruments. We further found that clinical subtyping, deformity, leg length discrepancy and limited joint mobility were significantly associated with this patient stratification, with the cluster with poorer physical functioning and higher psychological depression showing higher percentage of more severe deformity. Analyses of longitudinal data suggested that patients tend to stay in the same psychological state, in both clusters. Adult patients also showed a continuous spectrum of self-evaluation that matches their clinical manifestations. Conclusion: By probing deep into the subjective quantitation of patient reported outcomes, our study demonstrates the diverse sociopsychological spectrum among OI patients that matches their objective clinical manifestations, which may serve as the basis for evaluating clinical interventions and help achieve better patient-centric medical practices and health maintenance.
Association and Interaction Between Dietary Patterns and Gene Polymorphisms in Liangshan Residents with Hyperuricemia
Authors: Li Tingting , Li Shuangjing , Tian Tian , Nie Zhichao , Xu Wangdong , Liu Longjian , Jia Hong 
Published: June 2021

Abstract
Background: Hyperuricemia (HUA) is caused by genetic and dietary factors. However, little is known about the ways in which diet and gene interactions influence HUA or the role of dietary patterns in HUA. Objectives: We aimed to explore the association between dietary patterns and HUA as well as dietary patterns and gene interactions in HUA among the Yi ethnic group of China. Methods: Food consumption information was collected using a semi-quantitative food frequency questionnaire. Vein blood samples were collected after overnight fasting from all participants and DNA was extracted from peripheral blood leukocytes. Dietary patterns were derived using factor analysis.Results: The study included 2646 participants aged 18–88 years with a HUA prevalence of 26.8%. Three major dietary patterns were identified and shown to be associated with HUA prevalence, with meat-based diets having a higher association with HUA than plant-based or regionally specific diets. The frequency of the T allele at ABCG2 rs2231142 and SLC2A9 rs11722228 loci was higher in HUA than in non-HUA populations. An additive interaction between a meat-based diet and the rs2231142 locus was associated with the risk of HUA. The relative excess risks of interaction (RERI), attributable proportion of interaction (AP), and synergy index (S) were 0.482 (95% CI 29 = 0.012,0.976),0.203 (95% CI = 0.033,0.374), 1.544 (95% CI = 1.012,2.355), respectively. Conclusions: The meat-based dietary pattern was associated with an increased risk of HUA. There was an additive interaction between a meat-based dietary pattern and the ABCG2 rs2231142 locus in study participants. Individuals with the rs2231142 T allele had a higher frequency of HUA than those with the rs2231142 GG allele. Individuals with the rs2231142 T allele but a healthy dietary pattern could have a reduced HUA risk.
Construction and Confirmatory Factor Analysis of Panoramic Quality Assessment Tool for Investigator-Initiated Trials
Authors: Lv Wenwen , Hu Tingting , Jiang Jiayuan , Qu Tiantian , Shen Enlu , Duan Jiacheng , Miu Xin , Zhang Weituo , Qian Biyun 
Published: June 2021

Abstract
Introduction. Investigator initiated trials (IITs) are laid on the optimization and improvement of already existing therapies or treatment approaches and attempts to answer problems faced by clinicians in their daily practice. However, the quality assessment for conducting these sometimes complex studies is scarce of evidence. Our study aims at developing a handy quality assessment tool for IITs and providing investigators with resources to conduct their studies effectively. Furthermore, patient safety and data quality will also be kept as top priorities.Data and methods. The framework of the quality assessment tool was based on the literature studies and accepted guidelines and the Delphi method. Confirmatory factor analysis (CFA) is used to further evaluate the validity and feasibility of the conceptual quality assessment tool. Results. The panorama quality assessment tool for IITs contains four critical quality attributes, including progress, quality, regulation, scientificity, and 13 observed quality indexes. 272 IITs were collected, finally, 252 IITs were included in the validity and feasibility assessment. The majority of the studies (60.29%) were in Randomized Controlled Trial (RCT), and 41.18% of the studies were multi-center. In order to test for validity and feasibility of IITs quality assessment tool, CFA method was showed a fit for the model (CMIN/DF=1.868, GFI=0.916; CFI=0.936; TLI=0.919; RMSEA=0.063). Multiple types of studies of clinical studies, including RCT, prospective cohort study, retrospective, and real-world research fitted well in the tool by assessing their subject management, reasonable clinical research methods, and quality control methods. However, RCT ended with lower scores than prospective cohort study, retrospective and real-world research in enrollment progress (7.02 vs. 7.43, 9.63, 7.85, respectively). Conclusions. The panorama tool for overall quality assessment of IITs from this study combines the advantages of both qualitative and quantitative recognized evaluation indexes, which are further validated by CFA. The panorama evaluation tool can timely and dynamically find errors, take actions to prevent major bias. It is hoped that this framework can provide project management departments with resources for effective and dynamic management of their research and avoid waste of resources, as well as a manner to improve the quality of IIT in the future.