bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Cost-Effectiveness of Congenital Adrenal Hyperplasia Newborn Screening in Brazil: A Comparison Between Screened and Unscreened Cohorts
Authors: Miranda Mirela Costa de , Haddad Luciana Bertocco de Paiva , Trindade Evelinda , Cassenote Alex , Hayashi Giselle Y. , Damiani Durval , Costa Fernanda Cavalieri , Madureira Guiomar , Mendonca Berenice Bilharinho de , Bachega Tania A. S. S. 
Published: May 2021

Background: Newborn screening for congenital adrenal hyperplasia (CAH-NBS) is not yet a worldwide consensus, in part due to inconclusive evidence regarding cost-effectiveness because the analysis requires an understanding of the short- and long-term costs of care associated with delayed diagnosis.Objective: The present study aimed to conduct a cost-effectiveness analysis (CEA) to compare the costs associated with CAH-NBS and clinical diagnosis.Methods: A decision model comparing the two strategies was tested by sensitivity analysis. The cost analysis perspective was the public health system. Unscreened patients' data were extracted from medical records of Hospital das Clinicas, Saõ Paulo, and screened data were extracted from the NBS Referral Center of São Paulo. The population comprised 195 classical patients with CAH, clinically diagnosed and confirmed by hormonal/CYP21A2 analysis, and 378,790 newborns screened during 2017. Adverse outcomes related to late diagnosis were measured in both cohorts, and the incremental cost-effectiveness ratio (ICER) was calculated. We hypothesized that CAH-NBS would be cost-effective.Results: Twenty-five screened infants were confirmed with CAH (incidence: 1:15,135). The mortality rate was estimated to be 11% in unscreened infants, and no deaths were reported in the screened cohort. Comparing the unscreened and screened cohorts, mean serum sodium levels were 121.2 mEq/L (118.3–124.1) and 131.8 mEq/L (129.3–134.5), mean ages at diagnosis were 38.8 and 17 days, and hospitalization occurred in 76% and 58% of the salt-wasting patients with the in the two cohorts, respectively. The NBS incremental cost was US$ 771,185.82 per death averted, which yielded an ICER of US$ 25,535.95 per discounted life-year saved.Conclusions: CAH-NBS is important in preventing CAH mortality/morbidity, can reduce costs associated with adverse outcomes, and appears cost-effective.
The Effect of Free Androgen Index on the Quality of Life of Women With Polycystic Ovary Syndrome: A Cross-Sectional Study
Authors: Abdalla Mohammed Altigani , Deshmukh Harshal , Mohammed Irfaan , Atkin Stephen , Reid Marie , Sathyapalan Thozhukat 
Published: May 2021

Purpose: Free androgen index (FAI) and anti-Mullerian hormone (AMH) are independently associated with polycystic ovary syndrome (PCOS). This study aimed to describe the relationship between these two markers and health-related quality of life (HR-QoL) in women with PCOS.Methods: This cross-sectional study consisted of 81 women in the Hull PCOS biobank, who fulfilled the Rotterdam consensus criteria for the diagnosis of PCOS. The primary outcome was to measure the various domains of the QoL in the modified polycystic ovary syndrome questionnaire (MPCOSQ).Results: Mean age of the study participants was 28 ± 6.0 years, mean body mass index (BMI) 33.5 ± 7.8 kg/m2, mean FAI (6 ± 5.5), free testosterone (2.99 ± 0.75) and mean AMH (3.5 ± 0.8 units). In linear regression analysis, the FAI was associated with overall mean MPCOSQ score (Beta = 0.53, P-value = 0.0002), and with depression (Beta = 0.45, P-value = 0.01), hirsutism (Beta = 0.99, P-value = 0.0002) and menstrual irregularity (Beta = 0.31, P-value = 0.04). However, with adjustment for age and BMI, FAI was only associated with the hirsutism domain (Beta = 0.94, P-value = 0.001) of the MPCOSQ. FAI was also associated with the weight domain (Beta = 0.63 P-value = 0.005) of MPCOSQ. However, AMH was not associated with the overall mean MPCOSQ score or with any of its domains.Conclusion: FAI but not AMH was associated with QoL in women with PCOS, and this effect was mediated by BMI.
The Association Between Social Support, COVID-19 Exposure, and Medical Students' Mental Health
Authors: Yin Yi , Yang Xingjie , Gao Lan , Zhang Suoyuan , Qi Meng , Zhang Ligang , Tan Yunlong , Chen Jingxu 
Published: May 2021

Background: The coronavirus disease−2019 (COVID-19) pandemic has halted in-person medical education worldwide. Limited studies have reported on the mental health status of medical students during this public health emergency. This study aimed to explore the association of personal virus exposure, regional epidemic condition, and social support with medical students' depressive and anxiety symptoms during the COVID-19 outbreak in China.Methods: In February 2020, 5,982 medical students (60.0% females, Meanage = 21.7 years, Medianage = 22 years) completed an online survey consisting of demographics, personal virus exposure, the Patient Health Questionnaire, the Generalized Anxiety Disorder Scale, and the Social Support Rating Scale.Results: The prevalence rates of mild to severe depressive symptoms and anxiety symptoms were 35.2 and 22.8%, respectively. Multivariate linear regression showed that students with low- or medium-level social support had a higher risk of experiencing depressive or anxiety symptoms than those with high-level social support. COVID-19 exposure was positively associated with mild to severe depressive or anxiety symptoms. Respondents living in provinces with 500–1,000 confirmed COVID-19 cases had an increased risk of experiencing mild to severe depressive symptoms compared with those living in provinces with &lt;100 cases. Other related factors were gender and years of training.Conclusions: Some medical students suffered from a poor psychological status during the COVID-19 outbreak. Low social support was a stronger factor related to poor mental status compared with COVID-19 exposure or the provincial epidemic condition. Thus, we suggest that colleges or universities provide social support and mental health screening.
Problematic Use of Internet-Related Activities and Perceived Weight Stigma in Schoolchildren: A Longitudinal Study Across Different Epidemic Periods of COVID-19 in China
Authors: Fung Xavier C. C. , Siu Andrew M. H. , Potenza Marc N. , O'Brien Kerry S. , Latner Janet D. , Chen Chao-Ying , Chen I-Hua , Lin Chung-Ying 
Published: May 2021

Background:Social distancing and school suspension due to the coronavirus pandemic (COVID-19) may have a negative impact on children's behavior and well-being. Problematic smartphone use (PSU), problematic social media use (PSMU) and perceived weight stigma (PWS) are particularly important issues for children, yet we have a poor understanding of how these may have been affected by lockdowns and physical isolation resulting from COVID-19. This research aimed to understand how these psychosocial and behavioral variables may be associated with psychological distress, and how these associations may have changed during the COVID-19 pandemic.Methods:A total of 489 children completed a three-wave longitudinal study from January 2020 to June 2020. The first wave was conducted before the COVID-19 outbreak. The second wave was conducted during the outbreak. The third wave was conducted during post-COVID-19 lockdown. Questionnaires measured psychological distress, PSU, PSMU, and PWS.Results:PSU, PSMU, PWS and psychological distress were all significantly associated with each other. PSU was significantly higher during outbreak. PWS was significantly higher before outbreak. We found an increased association between PSMU and PWS across three waves in all three models. The association between PSU and depression/anxiety decreased across three waves; however, association between PSMU and depression/anxiety increased across three waves.Conclusions:COVID-19 initiated school suspension and associated lockdowns appear to have exacerbated PSU and depression among children. However, PWS was reduced during this period. Children should use smartphones and social media safely and cautiously, and be aware of the potential exposure to weight stigmatization.
Five Tetramic Acid Derivatives Isolated from the Iranian Fungus Colpoma quercinum CCTU A372
Authors: Primahana Gian , Narmani Abolfazl , Surup Frank , Teponno Rémy Bertrand , Arzanlou Mahdi , Stadler Marc 
Published: May 2021

Submerged mycelial cultures of the ascomycete Colpoma quercinum CCTU A372 were found to produce five previously undescribed tetramic acids, for which we propose the trivial names colposetins A–C (1–3) and colpomenoic acids A and B (4 and 5), along with the known compounds penicillide (6) and monodictyphenone (7). The planar structures of 1–5 were determined by high-resolution electrospray ionization mass spectrometry (HR-ESIMS) and extensive 1D and 2D nuclear magnetic resonance (NMR) spectroscopy. Their absolute configurations were determined by the combination of electronic circular dischroism (ECD) analysis, J-based configurational analysis, and a rotating-frame Overhauser effect spectroscopy (ROESY) experiment. Colposetin B displayed weak antimicrobial activity against Bacillus subtilis and Mucor hiemalis (MIC 67 µg/mL).
Mindfulness Meditation as Adjunctive Therapy to Improve the Glycemic Care and Quality of Life in Patients with Type 1 Diabetes
Authors: Shukla Rishi , Gupta Manisha , Agarwal Neha , Bajpai Anurag 
Published: May 2021

Background: Mindfulness Meditation (MM) is known to improve glycemic control and enhance the quality of life (QoL) in type 2 diabetes (T2D) patients. Unfortunately, the role of meditation in type 1 diabetes (T1D) has not been studied extensively. Therefore, we conducted this study to determine the effect of MM on the glycemic control and QoL in people living with T1D. Methodology: Thirty-two adults living with T1D were equally randomized into intervention (meditation) and control groups. The glycemic control and QoL were assessed at the baseline and after six months of intervention. Results: A total of thirty-two adults (15 males, 46.9%; mean age 23.8 ± 6.6 years) with type 1 diabetes (mean diabetes duration 12.7 ± 6.2 years) participated in the study. At the end of six months, a statistically significant improvement was seen in the mean blood glucose level in the control group (222.4 ± 77.8 versus 182.6 ± 52.0; p = 0.007) and the intervention group (215.3 ± 50.1 versus 193.2 ± 31.8; p = 0.008). Additionally, there was a significant reduction in the total diabetes distress score in the intervention group (1.6 ± 0.3 versus 1.3 ± 0.3; p = 0.003), while no change was observed in the control group (1.6 ± 0.7 versus 1.7 ± 0.4; p = 0.762). A statistically significant improvement was noticed in the health and functioning domain in the intervention group (p = 0.023). Conclusions: An improvement in the glycemic control and quality of life of the patients was observed in our study. MM certainly plays an important role in attaining peace of mind and helps patients to channel their energy in a positive direction.
Production of polyclonal antibodies against Turkey FMDV strains
Authors: SAREYYÜPOĞLU Beyhan 
Published: May 2021

Antibodies(Abs) have been always a major place in diagnostic laboratories. Many diagnostic techniques like Enzyme-Linked Immuno Sorbent Assays (ELISA), immunofluorescence, Ab-microarray platforms, immunoblots, X-ray crystallography require the Abs. ELISA is the main test that used Abs for Foot and Mouth Disease (FMDV) serology. It needs polyclonal or monoclonal Abs to detect FMDV antigen or Abs. For this purpose, solid-phase competitive ELISA (SPCE) or liquid phase blocking ELISA (LPBE) and non-structural protein (NSP) ELISA are used. SPCE and LPBE have mainly used FMDV structural protein (SP)-Ab detection.
In this study, it was aimed to produce a polyclonal Ab against FMDV ANep84 (Genotype VII) and OTUR07 (OPanAsia II), ATUR11 (A Iran05) strains for LPBE, FMDV SP-antibody detection. For this purpose, 4 guinea pigs and 6 rabbits were used for each serotype of FMDV. After producing Abs, checkerboard ELISA titration was performed to determine the optimal test dilution of Abs. Backgrounds, cross-reactions against three strains of FMDV were also checked. In conclusion, polyclonal Abs were produced against FMDV ANep84 (Genotype VII) and O Tur07 (O Pan Asia II), ATUR11 (A Iran05) strains, and standardized for LPBE test.
INCIDENCE OF MYCOTOXINS IN MOULDY SMOKED DRIED FISH AND MEAT (KUNDI) MARKETED IN SOUTHWESTERN NIGERIA
Authors: Wambai Fatima O. , Awotula Olusola , Oyedele Oluwapelumi A. , Ezekiel Chibundu N. , Akintunde Adeyinka O. , Tayo Grace O. 
Published: May 2021

The mycotoxicological safety of 85 mouldy dried fish (n=40) and meat (n=45) samples purchased from Ijebu-ode, Ogun State and Aleshinloye, Ibadan, Oyo State markets both in South Western part of Nigeria respectively, was assessed due to heavy consumption of these animal protein sources by many low-income families in Nigeria. The presence of aflatoxigenic moulds and levels of aflatoxins in the samples were determined by the dilution plating technique and high performance thin-layer chromatographic method. The predominant fungal species isolated from the samples was Aspergillus niger-clade (49.65%); other species isolated include Aspergillus section Flavi (36.83%) and (13.52%). All samples analysed for mycotoxin presence were contaminated with aflatoxin B1. Aflatoxin B2, G1 and G2 were below detectable limits. About 13% of the samples had aflatoxin B1 concentration higher than the maximum acceptable level (10 ng/g). Results imply that the consumption of mouldy dried meat can result in serious public health hazard and hence there is need for advocacy programs to enlighten the populace on proper processing and storage of meat products.
Trace Element Geochemistry and Mineral Inclusions Constraints on the Petrogenesis of a Marble–Hosted Ruby Deposit in Yunnan Province, China
Authors: Huang Wenqing , Ni Pei , Shui Ting , Pan Junyi , Fan Mingsen , Yang Yulong , Chi Zhe , Ding Junying 
Published: May 2021

ABSTRACT
Primary rubies in the Ailao Shan of Yunnan Province, China, are found in three layers of marble. However, the origin and source rocks of placer rubies in the Yuanjiang area remains unclear. Trace element geochemistry and inclusion mineralogy within these materials can provide information on their petrogenesis and original source. Zircon, rutile, mica group minerals, titanite, and apatite group minerals were the main solid inclusions identified within the placer Yuanjiang rubies, along with other mineral inclusions such as pyrite, pyrrhotite, plagioclase group minerals, and scapolite group minerals. Laser ablation inductively coupled plasma-mass spectrometry (LA-ICP-MS) measurements showed that the placer rubies are characterized by average values of Mg (31 ppmw), Ti (97 ppmw), V (77 ppmw), Cr (3326 ppmw), Fe (71 ppmw), and Ga (66ppmw). A trace-element oxide diagram, Fe values (&lt;350 ppmw), and the mineral inclusion assemblage suggest marble sources for the placer ruby. Therefore, the Yuanjiang rubies (both primary and placer) are metamorphic, and this fits well with the observations that skarn and related minerals are mostly absent in this deposit.
Yuanjiang rubies can be readily separated from the high-iron rubies of different geological types by their Fe content (&lt;1000 ppmw). The discriminators Mg, Ga, Cr, V, Fe, and Ti have potential in separating Yuanjiang rubies from some other marble-hosted deposits, such as Snezhnoe. Nevertheless, geographic origin determination remains a challenge when considering the similarities in compositional features between the Yuanjiang rubies and rubies from some other marble-hosted deposits worldwide (e.g., Luc Yen). The presence of kaolinite group minerals and clusters of euhedral, prismatic zircon crystals in ruby suggest a Yuanjiang origin.
Contrastive intonation effects on word recall for information-structural alternatives across the sexes
Authors: Koch Xaver , Spalek Katharina 
Published: May 2021

AbstractFocus highlights the fact that contextual alternatives are relevant for the interpretation of an utterance. For example, if someone says: “The meeting is on TUESDAY,” with focus marked by a pitch accent on “Tuesday,” the speaker might want to correct the assumption that the meeting is on Monday (an alternative date). Intonation as one way to signal focus was manipulated in a delayed-recall paradigm. Recall of contextual alternatives was tested in a condition where a set of alternatives was evoked by contrastive intonation. A control condition used intonation contours reported for broad focus in German. It was hypothesized that contrastive intonation improves recall, just as focus-sensitive particles (e.g., ‘only’) do, compared to sentences without such particles. Participants listened to short texts introducing a list of three elements from taxonomic categories. One of the three elements was re-mentioned in a subsequent critical sentence, realized with either a broad (H+!H*) or with a contrastive intonation contour (L+H*). Cued recall of the list elements was tested block-wise. Results show that contrastive intonation enhances recall for focus alternatives. In addition, it was found that the observed recall benefit is predominantly driven by females. The results support the assumption that contextual alternatives are better encoded in memory irrespective of whether focus is expressed prosodically or by a focus-sensitive particle. The results further show that females are more sensitive to pragmatic information conveyed through prosody than males.
Improved Treatment of Host-galaxy Correlations in Cosmological Analyses with Type Ia Supernovae
Authors: Popovic Brodie , Brout Dillon , Kessler Richard , Scolnic Dan , Lu Lisa 
Published: May 2021

Abstract
Improving the use of Type Ia supernovae (SNe Ia) as standard candles requires a better approach to incorporate the relationship between SNe Ia and the properties of their host galaxies. Using a spectroscopically confirmed sample of ∼1600 SNe Ia, we develop the first empirical model of underlying populations for SNe Ia light-curve properties that includes their dependence on host-galaxy stellar mass; we find a significant correlation between stretch population and stellar mass (99.9% confidence) and a weaker correlation between color and stellar mass (90% confidence). These populations are important inputs to simulations that are used to model selection effects and correct distance biases within the BEAMS with Bias Correction (BBC) framework. Here we improve BBC to also account for SNe Ia-host correlations, and we validate this technique on simulated data samples. We recover the input relationship between SNe Ia luminosity and host-galaxy stellar mass (the mass step, γ) with a bias of 0.004 ±0.001 mag, which is a factor of 5 improvement over previous methods that have a γ bias of ∼0.02 ± 0.001 mag. We adapt BBC for a novel dust-based model of intrinsic brightness variations, which results in a greatly reduced mass step for data (γ = 0.017 ± 0.008) and for simulations (γ = 0.006 ± 0.007). Analyzing simulated SNe Ia, the biases on the dark energy equation of state, w, vary from Δw = 0.006(5) to 0.010(5) with our new BBC method; these biases are significantly smaller than the 0.02(5) w bias using previous BBC methods that ignore SNe Ia-host correlations.
Star–Gas Misalignment in Galaxies. II. Origins Found from the Horizon-AGN Simulation
Authors: Khim Donghyeon J. , Yi Sukyoung K. , Pichon Christophe , Dubois Yohan , Devriendt Julien , Choi Hoseung , Bryant Julia J. , Croom Scott M. 
Published: May 2021

Abstract
There have been many studies aiming to reveal the origins of the star–gas misalignment found in galaxies, but there still is a lack of understanding of the contribution from each formation channel candidate. We aim to answer the question by investigating the misaligned galaxies in the Horizon-AGN simulation. There are 27,903 galaxies of stellar mass M
* > 1010
M
⊙ in our sample, of which 5984 are in a group in the halo mass of M
200 > 1012
M
⊙. We have identified four main formation channels of misalignment and quantified their levels of contribution: mergers (35%), interaction with nearby galaxies (23%), interaction with dense environments or their central galaxies (21%), and secular evolution, including smooth accretion from neighboring filaments (21%). We found in the simulation that the gas, rather than stars, is typically more vulnerable to dynamical disturbances; hence, misalignment formation is mainly due to the change in the rotational axis of the gas rather than stars, regardless of the origin. We have also inspected the lifetime (duration) of the misalignment. The decay timescale of the misalignment shows a strong anticorrelation with the kinematic morphology (V/σ) and the cold gas fraction of the galaxy. The misalignment has a longer lifetime in denser regions, which is linked with the environmental impact on the host galaxy. There is a substantial difference in the length of the misalignment lifetime depending on the origin, and it can be explained by the magnitude of the initial position angle offset and the physical properties of the galaxies.
NFAT5 Amplifies Antipathogen Responses by Enhancing Chromatin Accessibility, H3K27 Demethylation, and Transcription Factor Recruitment
Authors: Lunazzi Giulia , Buxadé Maria , Riera-Borrull Marta , Higuera Laura , Bonnin Sarah , Huerga Encabo Hector , Gaggero Silvia , Reyes-Garau Diana , Company Carlos , Cozzuto Luca , Ponomarenko Julia , Aramburu José , López-Rodríguez Cristina 
Published: May 2021

Abstract
The ability of innate immune cells to respond to pathogen-associated molecular patterns across a wide range of intensities is fundamental to limit the spreading of infections. Studies on transcription responses to pathogen-activated TLRs have often used relatively high TLR ligand concentrations, and less is known about their regulation under mild stimulatory conditions. We had shown that the transcription factor NFAT5 facilitates expression of antipathogen genes under TLR stimulation conditions corresponding to low pathogen loads. In this study, we analyze how NFAT5 optimizes TLR-activated responses in mouse macrophages. We show that NFAT5 was required for effective recruitment of central effectors p65/NF-κB and c-Fos to specific proinflammatory target genes, such as Nos2, Il6, and Tnf in primary macrophages responding to low doses of the TLR4 ligand LPS. By contrast, NFAT5 was not required for p65/NF-κB recruitment in response to high LPS doses. Using the transposase-accessible chromatin with high-throughput sequencing assay, we show that NFAT5 facilitated chromatin accessibility mainly at promoter regions of multiple TLR4-responsive genes. Analysis of various histone marks that regulate gene expression in response to pathogens identified H3K27me3 demethylation as an early NFAT5-dependent mechanism that facilitates p65 recruitment to promoters of various TLR4-induced genes. Altogether, these results advance our understanding about specific mechanisms that optimize antipathogen responses to limit infections.