bluuebunny/crossref_metadata_2025_split huggingface dataset:
Thermohalobaculum Xanthum Gen. Nov., Sp. Nov., A Novel Moderately Thermophilic Bacterium Isolated From Mangrove Sediment
Authors: Pan Xinli , Li Fei , Li Zhe , Huang Yuanlin , Wang Qiaozhen , Huang Shushi , Hu Wenjin 
Published: July 2021

Abstract
A novel moderately thermophilic and halophilic bacterium, designated strain M0105T, was isolated from mangrove sediment collected in the Beibu Gulf, south China. The isolate is Gram-negative, non-motile and rod-shaped bacterium with smooth colonies of pale-yellow appearance. Growth occurs at 15-46℃ (optimum 37-40℃) and pH range of 6.0-10.0 (optimum pH 8.0-9.0). It required 1-7% NaCl (optimum 3-5%) for growth. Strain M0105T was affiliated to the family ‘Rhodobacteraceae’, sharing the highest 16S rRNA gene sequence similarity with Limibaculum halophilum CAU 1123T (96.8%). The major menaquinone Q-10 and the dominant unsaturated fatty acid (C18:1ω7) in this family were also detected in the strain M0105T. The genome sequence possesses a circular 4.1 Mb chromosome with a G+C content of 67.9%. Strain M0105T encoded many genes for cellular stress resistance and nutrient utilization, which could improve its adaptive capacity to the mangrove environment. Values of conserved proteins (POCP), average nucleotide identity (ANI), average amino acid identity (AAI) and DNA-DNA hybridization (dDDH) between the isolate and closely related species were below the proposed threshold for species discrimination. Information from phenotypic, chemotaxonomic and phylogenetic analyses proposed that strain M0105T should be assigned to a novel genus within the family ‘Rhodobacteraceae’. Thus, we suggested that the strain M0105T represents a novel species in a new genus, for which the name Thermohalobaculum xanthum gen. nov., sp. nov. is proposed. The type strain of the type species is M0105T (=BGMRC 2019T=KCTC 52118T =MCCC 1K03767T=NBRC 112057T).Six supplementary figures and two supplementary tables are available with the online version of this article.
New Method for Calculating Bottom Hole Pressure of Horizontal Well in Fracture-cavity Reservoir Based on Point Source Function
Authors: Liu Hailong 
Published: July 2021

Abstract
Accurate determination of unsteady bottom hole pressure helps to monitor and predict well production in real-time. On the premise of fully considering the seepage characteristics of carbonate rock, a new source function suitable for the seepage of carbonate rock is established. It enlarges the application scope of source function theory and lays a theoretical foundation for solving the seepage problem of carbonate rock. This paper presents the process of solving bottom hole pressure step by step. Step 1: Based on the triple media model, the Pedrosa permeability calculation formula is applied to establish the seepage model of the triple media reservoir considering the formation stress sensitivity. Step 2: By perturbation transform and Laplace transform, the point source function considering stress sensitivity in carbonate reservoir is obtained in Laplace space. The point source function in the infinite plate reservoir is obtained by the principle of mirror image and superposition. Step 3: The method of solving the horizontal well pressure under the constant pressure boundary is established. Through literature comparison and numerical simulation, the rationality of the proposed method is verified. Simultaneously, the sensitivity analysis of pressure and pressure derivative is carried out, and the influences of fracture number, fracture angle, fracture half-length, skin factor, horizontal well segment length, and horizontal well segment spacing on pressure and pressure derivative are analyzed in detail. Considering fracture orientation and stress sensitivity, we divide the triple media fracture-vuggy reservoir fluid flow into five stages. The number of fractures and fracture direction mainly affect stage C. In contrast, the length of horizontal subsection and skin factor mainly affect stage B. Stage D is more obvious when the fracture half-length and the horizontal sublevel interval of the horizontal well are small.
LCE Violation for the Relational to Quantum Transition
Authors: Gupta Chitradeep 
Published: July 2021

Abstract
Quantization historically was never as much a problem as it was a solution to a problem and the problem was the failure of the classical material evolution statement. Under the axiomatic assumption that quantum theory is founded in Heisenberg's principle and Feynman's evolution we show that the QM path integral exists at the negation of the evolution of local conservation of energy(LCE) which in its presence fails with arbitrarily many interference terms. Along with LCE violation we uncover another GR-QM contradiction between the local arrow of time and the uncertainty principle. Every contradiction ~(p) n (q)~ is also a transition in p changing to q. The problem is GR is also caught up in an implication trail and cannot go through multiple parallel changes for LCE violation in presence of the QM path integral. To improve the recovery we go to an alternate projection of GR that has a set of independent frame invariant statements with a Lorentz invariant distinction of space and time.
Validation of the Japanese Version of the Patterns of Activity Measure-Pain in Individuals with Chronic Pain
Authors: Enomoto Kiyoka , Adachi Tomonori , Mibu Akira , Tanaka Katsuyoshi , Fukui Sei , Nakanishi Miho , Iwashita Narihito , Sasaki Jun , Nishigami Tomohiko 
Published: July 2021

Abstract
Background

The Patterns of Activity Measure-Pain (POAM-P) is a self-report questionnaire that measures avoidance, overdoing, and pacing in individuals with chronic pain. We aimed to develop and confirm the psychometric properties of the Japanese version of the POAM-P(POAM-P-J) in Japanese individuals with chronic pain.
Methods

We recruited 147 Japanese individuals with chronic pain (106 women; mean age 64.89 ± 12.13 years). The individuals completed the POAM-P-J, the Brief Pain Inventory (BPI), and the Hospital Anxiety and Depression Scale (HADS). The following psychometric properties of the POAM-P-J were confirmed: structural validity, internal consistency, test-retest reliability, and concurrent validity.
Results

We tested factor structure via confirmatory factor analyses (CFA). We chose the 3-factor model with six covariances. The POAM-P-J’s internal consistency and test-retest reliability were acceptable to good (α = 0.79–0.86; ICC = 0.72–0.87). The avoidance and overdoing scales were positively associated with pain severity, pain interference, and anxiety measures (all p < 0.05), but the pacing scale was not significantly associated with these pain-related measures.
Conclusions

Although the structural validity of the POAM-P-J remains questionable, its internal consistency, test-retest reliability, and concurrent validity were confirmed. The POAM-P-J is useful in both research and clinical practice for evaluating the activity patterns of Japanese patients with chronic pain.
Optimal pricing and complex analysis with carbon emission reduction preference in an apparel supply chain
Authors: Ma Junhai , Wang Zongxian 
Published: July 2021

Abstract
In this paper, we study the supply chain competition models that consider consumers' low-carbon consumption preferences. By constructing the supply chain of manufacturers and retailers with different market positions, two game types, decentralized decision making and centralized decision making are proposed, and the static and dynamic game methods are combined respectively for research. Although research has found that centralized decision making is always better than decentralized decision making under the static game mode, when considering the long-term dynamic evolutionary game, the average profit of decentralized decision making may be better than the average profit of centralized decision making. Moreover, there are complex dynamic characteristics under dynamic games. In order to fully understand the nature of the dynamic game systems, we discussed the stability, local bifurcation, and global bifurcation of these dynamic systems. It is found that although dynamic game systems may lose stability with changes in parameters, they are better than static game results in some cases, this is beneficial to decision-makers and has practical management significance.
Short-term Effects of Multiple Ozone Metrics on Outpatient Visits For Urticaria in Lanzhou
Authors: Zhang Jing , He Yuan , Shi chunrui 
Published: July 2021

Abstract
Background Although mounting evidence suggests that short-term exposure to ozone increases the risk of respiratory disease, cardiovascular disease and mortality, there are few studies comparing the effects of ozone in relation to urticaria in China.Objective To evaluate the risks for urticaria exacerbations related to ambient ozone measured as 1-h maximum (O3-1 h max), maximum 8-h (O3-8 h max) and 24-h average (O3-24 h avg) concentrations. Methods We calculated three metrics of ozone, 1-h maximum, maximum 8-h and 24-h average based on the hourly data. Generalized additive models with poisson regression incorporating natural spline functions were used to investigate short-term effects on urticaria associated with ambient ozone pollution in Lanzhou, China, using 5 years of daily data (2013-2017). We also examined the association by sex, age and season.Results In all-year analyses, a 10 μg/m3 increase in daily average, O3-1 h max, O3-8 h max and O3-24 h avg at lag2 corresponded to an increase of 0.58%(95%CI：0.26%~0.90%), 0.82% (95%CI：0.47%~1.16%) and 2.17% (95%CI：1.17%~2.79%), respectively. The elderly populations and females were susceptible to O3, and the associations between ozone and urticaria appeared to be more evident during warm season than in the cold season.Conclusion In conclusion, these results indicated that ozone, as a widespread pollutant, affects outpatient visits for urticaria in Lanzhou.
SNARER: New Molecular Descriptors for SNARE Proteins Classification
Authors: Citarella Alessia Auriemma , Biasi Luigi Di , Risi Michele , Tortora Genoveffa 
Published: July 2021

Abstract
Background: SNARE proteins play an important role in different biological functions. This study aims to investigate the contribution of a new class of molecular descriptors (called SNARER) related to the chemical-physical properties of proteins in order to evaluate the performance of binary classifiers for SNARE proteins. Results: We constructed a SNARE proteins balanced dataset, D128, and an unbalanced one, DUNI, on which we tested and compared the performance of the new descriptors presented here in combination with the feature sets (GAAC, CTDT, CKSAAP and 188D) already present in the literature. The machine learning algorithms used were Random Forest, k-Nearest Neighbors and AdaBoost and oversampling and subsampling techniques were applied to the unbalanced dataset. The addition of the SNARER descriptors increases the precision for all considered ML algorithms. In particular, on the unbalanced DUNI dataset the accuracy increases in parallel with the increase in sensitivity while on the balanced dataset D128 the accuracy increases compared to the counterpart without the addition of SNARER descriptors, with a strong improvement in specificity. Our best result is the combination of our descriptors SNARER with CKSAAP feature on the dataset D128 with 92.3% of accuracy, 90.1% for sensitivity and 95% for specificity with the RF algorithm. Conclusions: The performed analysis has shown how the introduction of molecular descriptors linked to the chemical-physical and structural characteristics of the proteins can improve the classification performance. Additionally, it was pointed out that performance can change based on using a balanced or unbalanced dataset. The balanced nature of training can significantly improve forecast accuracy.
Robust Power Allocation for NOMA Heterogeneous Networks with EH under Imperfect CSI
Authors: Song Xin , Huang Xue , Gao Yiming , Qian Haijun 
Published: July 2021

Abstract
A robust power allocation is proposed for downlink non-orthogonal multiple access (NOMA) heterogeneous networks with EH (Energy harvesting) under imperfect channel state information (CSI). In order to achieve green communication, an EH-aided scheme by leveraging energy from macro base station (MBS) signal and interference signal transmitted from other SBSs is proposed, which reduces the power burden and energy consumption of the SBS. In order to conform to the actual communication scenario, we construct an energy efficiency optimization function under imperfect CSI with considering the constraint of the outage probability interference power in macro cell user (MCU). However, the formulated optimization problem is non-convex due to the fractional form of the objective function and the probabilistic constraints of the outage probability limit. To cope with this problem, we propose a robust power allocation scheme. Firstly, the probabilistic problem is converted into a robust non-probabilistic problem by the minimax probability machine (MPM) and robust optimization theory. Then, the robust non-probabilistic problem can be transformed into the convex optimization problem via Dinkelbach method and sequential convex programming. Finally, the optimal transmission powers of the small cell users (SCUs) are obtained by Lagrange dual approach. The simulation results show that the robust power allocation scheme for NOMA heterogeneous networks with EH under imperfect CSI can significantly improve energy efficiency compared with traditional power allocation algorithms.
Human epidermal growth factor-expressing mesenchymal stem cells reduce matrix metalloproteinase-1 expression via the mitogen-activated protein kinase pathway in human keratinocyte HaCaT cells
Authors: Lee Jeong Hyun , Lee Chang Min , Choi Yong Jun , Park See-Hyoung , Nam Myeong Jin 
Published: July 2021

Abstract
Mesenchymal stem cells (MSCs) repair tissue injury by upregulating the paracrine secretion of cytokines and growth factors. In this study, we examined the effect of human epidermal growth factor-transfected MSCs (hEGF MSCs) on human keratinocyte HaCaT cell proliferation and the mechanisms that regulate matrix metalloproteinase (MMP)-1 expression in HaCaT cells. To identify the hEGF plasmid and its transfection into MSCs, we performed gel electrophoresis and quantitative PCR. Proliferation and migration of HaCaT cells were examined using WST-1 and wound-healing assays, respectively. Zymography was performed to investigate the correlation between hEGF MSC-conditioned medium (CM)-treated HaCaT cells and MMP-1 expression. We found that cell viability and wound-healing rates were increased in hEGF MSC-CM-treated HaCaT cells compared to those in MSC-CM-treated cells, and conversely collagenase activity was decreased. The mRNA and protein levels of MMP-1 were also decreased in hEGF MSC-CM-treated HaCaT cells. 2-DE analysis showed that the expression of carboxypeptidase, which promotes growth factors and wound healing, was increased in hEGF MSC-CM-treated HaCaT cells. Finally, western blotting was used to determine whether MMP-1 expression was reduced via the mitogen-activated protein kinase (MAPK) pathway; the results showed that the levels of MAPK pathway-related proteins (pErk, pJNK, and p-p38) and the levels of transcription factors (pCREB, NFκB, and p-c-Fos) were decreased. In addition, pAkt expression was found to be elevated. The results of our study suggest that hEGF MSCs promote cell proliferation and reduce MMP-1 expression via the MAPK pathway in human keratinocyte HaCaT cells.
Transcriptome analysis identified core genes involved in maize resistance to Rhizoctonia solani
Authors: Cao Hongxiang , Yang Zhangshuai , Song Shu , Liu Jiazong , Li Ning 
Published: July 2021

Abstract
Banded leaf and sheath blight (BLSB) caused by the necrotrophic fungus Rhizoctonia solani is a devasting disease on maize worldwide, especially in China and Southeast Asia. To explore the maize defense mechanisms against R. solani expansion, the expression profile of maize infected by low virulence strain (LVS) and high virulence strain (HVS) of R. solani for 3 and 5 d was analyzed by RNA-sequencing. A total of 3015 and 1628 differentially expressed genes (DEGs) were identified under LVS and HVS infection, respectively. Meanwhile, these DEGs were classified by Gene Ontology (GO) for biological process analysis. Only defense-related GO terms were commonly enriched in LVS- and HVS-regulated genes. Furthermore, a core set of 388 up-regulated genes that are involved in maize response to R. solani infection were identified. Additionally, among the core genes, overexpressing ZmNAC41 and ZmBAK1 enhanced rice resistance to R. solani. Taken together, our study provides additional insight into maize defense mechanisms against R. solani, and the core genes identified in this study will be important resources for improving BLSB resistance in the future.
miR-375-NAT10 Axis Dysfunction Promotes Oral Cancer Development and Mediates Cdk7 Stabilizing and Cell Cycle Progression
Authors: ZOU Chen , LI Xia , WEI Haigang , WU Siyuan , SONG Jing , TANG Zhe , LUO Hailing , LV Xiaozhi , Ai Yilong 
Published: July 2021

Abstract
Background: Oral cancer is the most common cancer with poor prognosis and outcome for the patients due to the challenging diagnosis and limited treatment possibilities. However, the molecular underpinnings behind the malignant progression of oral cancer remain incompletely understood. Methods: The expression profiling of NAT10 and CDK7 in oral cancer patients were assessed by IHC, qPCR and western blots. ShRNA was used to silence gene expression. The biological function of NAT10 and CDK7 in cholangiocarcinoma was investigated using in vitro and in vivo studies including, transwell cell migration, plate cloning, CCK8, shRNA interference, western blots, flow cytometry and xenograft mouse model. The underlying molecular mechanism was determined by western blots and immunoprecipitation.Results: In this study, we demonstrated that deregulation of miR-375-NAT10 axis is among the most causes in inducing the acquisition of a tumorigenesis phenotype in oral cancer cells. NAT10 is abundant in oral cancer tissue. and its protein level is positively correlated with poor overall survival. Increased the level of NAT10 promotes oral cancer cell proliferation in vitro as well as xenograft tumorigenicity in vivo. Most importantly, NAT10 regulates cancer cell proliferation through stabilizing CDK7 thus regulating the cell cycle. NAT10 as an acetyltransferase is responsible for CDK7 acetylation at lysine 328 (K328Ac). Moreover, it was found that the expression of miR-375 is abnormally alleviated in oral cancer tissues. Bioinformatics analysis revealed a targeted complementary binding site between miR-375 and NAT10. Decreased expression of miR-375 promotes expression of NAT10.Conclusion: Our study showed that NAT10 plays a strong carcinogenic role in oral cancer tumorigenesis by acetylating CDK7 at K382 thus promotion stability. Moreover, NAT10 may serve as a target for miR-375. Therefore, targeting NAT10 may provide a new and effective therapeutic strategy to inhibit the tumorigenicity of oral cancer.
Treatment Strategy of Lung Adenocarcinoma with Concomitant EGFR Mutation and ALK Rearrangement
Authors: Li Li , Liu Wei , Xu Chunhua , Zou Jue 
Published: July 2021

Abstract
Background

The incidence of synchronous mutations of Epidermal growth factor receptor (EGFR) and anaplastic large-cell lymphoma kinase (ALK) rearrangements in non-small cell lung cancer (NSCLC) was low. Now clinical experience is still insufficient. Simultaneously the treatment of brain metastasis hemorrhage in the acute phase with lung cancer is still controversial. We described the clinical treatment strategy of a patient with synchronous mutations of EGFR and ALK.
Methods

The patient was a 55-year-old man with a mass in the right lower lobe. Pathological examination confirmed adenocarcinoma, tissue molecular examination showed EGFR exon 19 deletion, and ALK rearrangement. The patient received pemetrexed combined with cisplatin chemotherapy, gefitinib targeted therapy, clotriminib targeted therapy, albumin paclitaxel combined with nedaplatin and anlotinib therapy, and seretinib therapy. In the course of treatment, the patients had sudden tumor emergency, extensive hemorrhage and edema of brain metastasis, paralysis occurred in the patients. Subsequently, targeted therapy with ceritinib was given. After 1 month, lung tumors, brain metastases, and cerebral hemorrhage were all significantly improved.
Results

The tumor was well controlled. Progression-free survival (PFS)1 was 4 months, PFS2 was 3 months, PFS3 was 5 months, PFS4 was 5 months, and PFS5 was 9 months. At present, the patient still maintains partial response (PR) status.
Conclusions

Patients with simultaneous mutations choose the correct treatment strategy, which can significantly benefit the patients' PFS and quality of life. Especially for patients with acute hemorrhage of brain metastases, oral ceritinib may be an effective choice.
Long Noncoding RNA TCONS_00027385 Acts as a miR-874-5p Sponge to Suppress the Progression of Prostate Cancer Through Regulating ASCC2 Expression
Authors: han jianxin , Tao Ning , Zhao Zhenlei , Gu Yanpei , Xue Fan , Yan Yali , Chen Lihuan , Xiao Hongrui , Qiu Ruiying , Zhang Ying , An Hengqing , Li Wei 
Published: July 2021

Abstract
Background: A novel pyrrolo indole alkaloids, named Robustanoids A, was isolated from Coffea canephora beans, and it inhibits proliferation of prostate cancer (PCa) cells. However, the molecular mechanism linking Robustanoids A to the tumorigenesis of PCa is not yet clear. Methods: We investigated the expression of lncRNAs in PCa cells with Robustanoids A and control group by microarray analysis. The expression level of TCONS_00027385 in PCa tissues and cell lines was detected by qRT-PCR. Additionally, we conducted functional experiments to investigate the biological effects of TCONS_00027385 on the development of PCa both in vitro and in vivo. Furthermore, bioinformatic analysis, luciferase reporter experiment, RIP assay, pulldown assay, and protein chip were performed to investigate the oncogenic molecular mechanisms of TCONS_00027385.Results: In our current study, we focused on TCONS_00027385, which was up-regulated in PCa tissues and cell lines. The high expression of TCONS_00027385 was related to the progression of PCa. Function assays revealed that silencing TCONS_00027385 inhibited PCa cell proliferation and induced apoptosis, while over-expression of TCONS_00027385 remarkably played an opposite role. A deeper investigation showed that TCONS_00027385 acted as a sponge for hsa-miR-874-5p in PCa, and ASCC2 was a target of miR-874-5p in the downstream. Moreover, a positive association between TCONS_00027385 with ASCC2 and a negative relationship between miR-874-5p and TCONS_00027385 (or ASCC2) were also founded. According to the rescue assay, inhibiting ASCC2 could partially suppress the oncogenic effect on cell proliferation and apoptosis in PCa caused by the overexpression of TCONS_00027385.Conclusion: TCONS_00027385 acted as a competing endogenous RNA (ceRNA) for miR-874-5p to regulate the expression of ASCC2. TCONS_00027385 regulated the miR-874-5p/ASCC2 axis to promote PCa progression.