bluuebunny/crossref_metadata_2025_split huggingface dataset:
Hormonal protocol used for cervical dilation in ewes does not affect morphological embryo quality but reduces recovery rate and temporarily alters gene expression
Authors: Santos Juliana Dantas Rodrigues , Batista Ribrio Ivan Tavares Pereira , Ungerfeld Rodolfo , Taira Augusto Ryonosuke , Espírito Santo Caroline Gomes do , Souza‐Fabjan Joanna Maria Gonçalves , Fernandes Daniel Andrews de Moura , Balaro Mário Felipe Alvarez , Cosentino Isabel Oliveira , Brair Viviane Lopes , Pinto Pedro Henrique Nicolau , Carvalho Ana Beatriz da Silva , Fonseca Jeferson Ferreira da , Brandão Felipe Zandonadi 
Published: November 2021

AbstractBackgroundInformation on the impact of hormonal protocols for cervical dilation on the quality of ovine embryos is scarce.MethodsTo compare the quality of embryos after cervical dilation protocol, ewes (n = 64) were allocated into either a treated group (100 μg estradiol benzoate intravenous and 0.12 mg cloprostenol intramuscularly, 12 hours before embryo collection plus 100 iu oxytocin intravenous 15 minutes before the collection procedure) or a control group (saline). Luteal function was analysed using ultrasonography and P4 measurement. Some collected embryos were frozen/thawed for gene expression, others were cultured in vitro, frozen/thawed for gene expression, and the remaining embryos were fixed for the apoptosis test (TUNEL test).ResultsThe treatment reduced fluid (p=0.04) and structure (p=0.03) recovery rates, but the morphological quality, development stage, and apoptosis incidence of the embryos were not affected by treatment. The corpora lutea of the control group had greater blood perfusion (p = 0.002) and greater P4 concentrations at 6, 9, and 12 h after the treatment (p < 0.0001). The expression of BAX, BCL2, PRDX1, and HSP90 genes were not affected by the treatment. However, the embryos in the treated group had fewer NANOG and OCT4 transcripts than control embryos (p = 0.008; p = 0.006, respectively). After culture, there was no difference between the groups in any gene.ConclusionThe hormonal protocol for cervical dilation reduced the efficiency of embryo collection. In addition, the treatment induced luteolysis and a transient alteration of embryo gene expression, however there were no detectable changes in embryo morphological quality, development stage, or incidence of apoptosis.
Analytical performance of Compton/Rayleigh signal ratio by total reflection X‐ray fluorescence (TXRF): A potential methodological tool for sample differentiation
Authors: Mennickent Daniela , Castillo Rosario del Pilar , Araya Juan , Neira José Yamil 
Published: November 2021

AbstractThe high sensitivity Compton and Rayleigh X‐ray scattering signals can be used to gain valuable information on the chemical composition of various matrices, by exploiting the ratio of those signals as a function of the effective atomic number (Zeff). Neither total reflection X‐ray fluorescence (TXRF) nor the effect of the experimental setup, including sample preparation, X‐ray excitation source selection, and band deconvolution procedure, has been assessed in this kind of approach. Here, a Compton/Rayleigh ratio and Zeff‐based TXRF method was set up and tested as an analytical tool for milk samples differentiation. The method was developed using a 90° scattering angle and assessed using different X‐ray excitation sources: a molybdenum tube (Mo Kα 17.5 KeV) and a tungsten tube (W Lα 8.5 KeV and W‐Brems 35 KeV). The evaluation of independent Compton and Rayleigh signals was performed by non‐Gaussian and Gaussian curve resolution methods, and both height and area‐based calculations were evaluated. Different sample preparation conditions were assessed. By using 11 standard materials, a calibration curve for Compton/Rayleigh ratio versus Zeff was established. The method was tested to determine the Zeff of milk samples, which enabled its use as a parameter to differentiate them. Good precisions were obtained with the Mo excitation source and the area‐based calculations, which allowed to differentiate undiluted milk samples by species, treatment, and fat content according to their Compton/Rayleigh ratio. This simple and rapid method has the potential to be used for the differentiation of various types of samples, including liquids, solids, and aerosols.
A falling magnetic monopole as a holographic local quench
Authors: Zenoni Nicolò , Auzzi Roberto , Caggioli Stefania , Martinelli Maria , Nardelli Giuseppe 
Published: November 2021

Abstract
An analytic static monopole solution is found in global AdS4, in the limit of small backreaction. This solution is mapped in Poincaré patch to a falling monopole configuration, which is dual to a local quench triggered by the injection of a condensate. Choosing boundary conditions which are dual to a time-independent Hamiltonian, we find the same functional form of the energy-momentum tensor as the one of a quench dual to a falling black hole. On the contrary, the details of the spread of entanglement entropy are very different from the falling black hole case, where the quench induces always a higher entropy compared to the vacuum, i.e. ∆S > 0. In the propagation of entanglement entropy for the monopole quench, there is instead a competition between a negative contribution to ∆S due to the scalar condensate and a positive one carried by the freely propagating quasiparticles generated by the energy injection.
Survey of clinical practice pattern in Germany’s certified chest pain units
Authors: Breuckmann Frank , Settelmeier Stephan , Rassaf Tienush , Post Felix , Haerer Winfried , Bauersachs Johann , Mudra Harald , Voigtländer Thomas , Senges Jochen , Münzel Thomas , Giannitsis Evangelos 
Published: November 2021

Abstract
Background
We aimed to analyze the 2020 standard of care in certified German chest pain units (CPU) with a special focus on non-ST-segment elevation acute coronary syndrome (NSTE-ACS) through a voluntary survey obtained from all certified units, using a prespecified questionnaire.

Methods
The assessment included the collection of information on diagnostic protocols, risk assessment, management and treatment strategies in suspected NSTE-ACS, the timing of invasive therapy in non-ST-segment elevation myocardial infarction (NSTEMI), and the choice of antiplatelet therapy.

Results
The response rate was 75%. Among all CPUs, 77% are currently using the European Society of Cardiology (ESC) 0/3‑h high-sensitive troponin protocol, and only 20% use the ESC 0/1‑h high-sensitive troponin protocol as a default strategy. Conventional ergometry is still the commonly performed stress test with a utilization rate of 47%. Among NSTEMI patients, coronary angiography is planned within 24 h in 96% of all CPUs, irrespective of the day of the week. Prasugrel is the P2Y12 inhibitor of choice in ST-segment elevation myocardial infarction (STEMI), but despite the impact of the ISAR-REACT 5 trial on selection of antiplatelet therapy, ticagrelor is still favored over prasugrel in NSTE-ACS. If triple therapy is used in NSTE-ACS with atrial fibrillation, it is maintained up to 4 weeks in 51% of these patients.

Conclusion
This survey provides evidence that Germany’s certified CPUs ensure a high level of guideline adherence and quality of care. The survey also identified areas in need of improvement such as the high utilization rate of stress electrocardiogram (ECG).
Towards an effective transnational regulation of AI
Authors: Gervais Daniel J. 
Published: November 2021

AbstractLaw and the legal system through which law is effected are very powerful, yet the power of the law has always been limited by the laws of nature, upon which the law has now direct grip. Human law now faces an unprecedented challenge, the emergence of a second limit on its grip, a new “species” of intelligent agents (AI machines) that can perform cognitive tasks that until recently only humans could. What happens, as a matter of law, when another species interacts with us, can be integrated into human minds and bodies, makes “real-world” decisions—not through human proxies, but directly—and does all this “intelligently”, with what one could call autonomous agency or even a “mind” of its own? The article starts from the clear premise that control cannot be exercised directly on AI machines through human law. That control can only be effected through laws that apply to humans. This has several regulatory implications. The article’s first discusses what, in any attempt to regulate AI machines, the law can achieve. Having identified what the law can do, the article then canvases what the law should aim to achieve overall. The article encapsulate its analysis in a list of both doctrinal and normative principles that should underpin any regulation aimed at AI machines. Finally, the article compares three transnational options to implement the proposed regulatory approach.
Effect of coronavirus (COVID-19) pandemic on orthopedic trauma patients presenting in the emergency department of a maximum care hospital and level 1 trauma center
Authors: Frink M. , Ketter V. , Klama N. , Knauf T. , Betz S. , Ruchholtz S. , Aigner R. 
Published: November 2021

Abstract
Introduction
While overcrowding of emergency departments was often reported in the recent years, during the early phase of the pandemic, a reduction in patient numbers was seen. The aim of the current study was to describe the orthopedic trauma patient cohort presenting to the emergency department (ED) during the early pandemic period as compared to the cohort from the analogue time period 2019.

Materials and methods
A single-center case–control study was performed. All the consecutive orthopedic trauma patients > 12 years presenting to the ED were included. Patients in the same time period in 2019 served as the control group.

Results
Compared to 2019, in 2020, 33% less patients presented in the emergency department. Patients treated in 2020 were significantly older, significantly more often brought to ED by emergency medical services and significantly more often admitted. The number of fractures and diagnoses requiring surgical treatment decreased only slightly and the proportion of these patients among all the patients was significantly higher during the pandemic than in the control period. Furthermore, a higher percentage of polytrauma patients could be found in 2020 as well. Analysis of Manchester Triage System showed significantly less not urgent patients in 2020.

Conclusion
The present study shows a significant decline in the number of patients treated in the ED during the pandemic period but at the same time almost identical numbers of patients with fractures or diagnoses requiring surgical treatment. In the context of an overall decline in patient numbers, a stronger concentration on level 1 trauma centers seems to be evident during the pandemic.
Exploring the limits of conventional small-scale CHO fed-batch for accelerated on demand monoclonal antibody production
Authors: Mahé Amélie , Martiné Alexandra , Fagète Séverine , Girod Pierre-Alain 
Published: November 2021

AbstractIn the field of therapeutic antibody production, diversification of fed-batch strategies is flourishing in response to the market demand. All manufacturing approaches tend to follow the generally accepted dogma of increasing titer since it directly increases manufacturing output. While titer is influenced by the biomass (expressed as IVCD), the culture time and the cell-specific productivity (qP), we changed independently each of these parameters to tune our process strategy towards adapted solutions to individual manufacturing needs. To do so, we worked separately on the increase of the IVCD as high seeding fed-batch capacity. Yet, as intensified fed-batch may not always be possible due to limited facility operational mode, we also separately increased the qP with the addition of specific media additives. Both strategies improved titer by 100% in 14 days relative to the standard fed-batch process with moderate and acceptable changes in product quality attributes. Since intensified fed-batch could rival the cell-specific productivity of a conventional fed-batch, we developed novel hybrid strategies to either allow for acceptable seeding densities without compromising productivity, or alternatively, to push the productivity the furthest in order to reduce timelines.
Dysphagia aortica
Authors: Grimaldi Serena , Milito Pamela , Lovece Andrea , Asti Emanuele , Secchi Francesco , Bonavina Luigi 
Published: November 2021

SummaryBackgroundDysphagia aortica is an umbrella term to describe swallowing obstruction from external aortic compression secondary to a dilated, tortuous, or aneurysmal aorta. We performed a systematic literature review to clarify clinical features and outcomes of patients with dysphagia aortica.Materials and methodsWe searched PubMed, EMBASE, Web of Science, and the Cochrane Library. The terms “aortic dysphagia,” “dysphagia aortica,” “dysphagia AND aortic aneurysm” were matched. We also queried the prospectively updated database of our esophageal center to identify patients with aortic dysphagia referred for diagnosis and treatment over the past two decades.ResultsA total of 57 studies including 69 patients diagnosed with dysphagia aortica were identified, and one patient from our center was added to the database. The mean age was 72 years (range 22–98), and the male to female ratio 1.1:1. Of these 70 patients, the majority (n = 63, 90%) had an aortic aneurysm, pseudoaneurysm, or dissection. Overall, 37 (53%) patients received an operative treatment (81.1% a vascular procedure, 13.5% a digestive tract procedure, 5.4% both procedures). Thoracic endovascular aortic repair (TEVAR) accounted for 60% of all vascular procedures. The postoperative mortality rate was 21.2% (n = 7/33). The mortality rate among patients treated conservatively was 55% (n = 11/20). Twenty-six (45.6%) studies were deemed at a high risk of bias.ConclusionDysphagia aortica is a rare clinical entity with high morbidity and mortality rates and no standardized management. Early recognition of dysphagia and a high suspicion of aortoesophageal fistula may be lifesaving in this patient population.
Prevalence of Masturbation and Associated Factors Among Older Adults in Four European Countries
Authors: Fischer Nantje , Graham Cynthia A. , Træen Bente , Hald Gert Martin 
Published: November 2021

AbstractSolitary sexual activity is a free, safe, and accessible way to experience sexual pleasure. Despite these advantages, research on masturbation in later life is highly understudied. Using data from a cross-sectional probability-based survey of 3816 European adults (mean age 67 years; range 60–75 years), we explored several sociodemographic, health, attitudinal, and sexual behavioral factors associated with reported masturbation frequency. Across all countries, between 41% and 65% of men and 27% and 40% of women reported any masturbation in the preceding month. Satisfaction with sexual activity and attitudes related to disapproval of sex without love were significant predictors of reported masturbation in almost all countries and in both genders. Age, education, self-perceived health, and depression were for the most part predictive of men’s reported masturbation, but not women’s. Generally, those believing sex is beneficial to older people were more likely to masturbate, while less permissive attitudes decreased the likelihood of reporting masturbation. To improve healthy sexual aging, misinformation about masturbation and sexual attitudes in older people need to be addressed.
Degradation of dibutyl phthalate by Paenarthrobacter sp. Shss isolated from Saravan landfill, Hyrcanian Forests, Iran
Authors: Shariati S. , Ebenau-Jehle C. , Pourbabaee A. A. , Alikhani H. A. , Rodriguez-Franco M. , Agne M. , Jacoby M. , Geiger R. , Shariati F. , Boll M. 
Published: November 2021

AbstractPhthalic acid esters are predominantly used as plasticizers and are industrially produced on the million ton scale per year. They exhibit endocrine-disrupting, carcinogenic, teratogenic, and mutagenic effects on wildlife and humans. For this reason, biodegradation, the major process of phthalic acid ester elimination from the environment, is of global importance. Here, we studied bacterial phthalic acid ester degradation at Saravan landfill in Hyrcanian Forests, Iran, an active disposal site with 800 tons of solid waste input per day. A di-n-butyl phthalate degrading enrichment culture was established from which Paenarthrobacter sp. strain Shss was isolated. This strain efficiently degraded 1 g L–1 di-n-butyl phthalate within 15 h with a doubling time of 5 h. In addition, dimethyl phthalate, diethyl phthalate, mono butyl phthalate, and phthalic acid where degraded to CO2, whereas diethyl hexyl phthalate did not serve as a substrate. During the biodegradation of di-n-butyl phthalate, mono-n-butyl phthalate was identified in culture supernatants by ultra-performance liquid chromatography coupled to electrospray ionization quadrupole time-of-flight mass spectrometry. In vitro assays identified two cellular esterase activities that converted di-n-butyl phthalate to mono-n-butyl phthalate, and the latter to phthalic acid, respectively. Our findings identified Paenarthrobacter sp. Shss amongst the most efficient phthalic acid esters degrading bacteria known, that possibly plays an important role in di-n-butyl phthalate elimination at a highly phthalic acid esters contaminated landfill.
The high-energy Sun - probing the origins of particle acceleration on our nearest star
Authors: Matthews S. A , Reid H. A. S. , Baker D. , Bloomfield D. S. , Browning P. K. , Calcines A. , Del Zanna G. , Erdelyi R. , Fletcher L. , Hannah I. G. , Jeffrey N. , Klein L. , Krucker S. , Kontar E. , Long D. M. , MacKinnon A. , Mann G. , Mathioudakis M. , Milligan R. , Nakariakov V. M. , Pesce-Rollins M. , Shih A. Y. , Smith D. , Veronig A. , Vilmer N. 
Published: November 2021

AbstractAs a frequent and energetic particle accelerator, our Sun provides us with an excellent astrophysical laboratory for understanding the fundamental process of particle acceleration. The exploitation of radiative diagnostics from electrons has shown that acceleration operates on sub-second time scales in a complex magnetic environment, where direct electric fields, wave turbulence, and shock waves all must contribute, although precise details are severely lacking. Ions were assumed to be accelerated in a similar manner to electrons, but γ-ray imaging confirmed that emission sources are spatially separated from X-ray sources, suggesting distinctly different acceleration mechanisms. Current X-ray and γ-ray spectroscopy provides only a basic understanding of accelerated particle spectra and the total energy budgets are therefore poorly constrained. Additionally, the recent detection of relativistic ion signatures lasting many hours, without an electron counterpart, is an enigma. We propose a single platform to directly measure the physical conditions present in the energy release sites and the environment in which the particles propagate and deposit their energy. To address this fundamental issue, we set out a suite of dedicated instruments that will probe both electrons and ions simultaneously to observe; high (seconds) temporal resolution photon spectra (4 keV – 150 MeV) with simultaneous imaging (1 keV – 30 MeV), polarization measurements (5–1000 keV) and high spatial and temporal resolution imaging spectroscopy in the UV/EUV/SXR (soft X-ray) regimes. These instruments will observe the broad range of radiative signatures produced in the solar atmosphere by accelerated particles.
Estimating heterogeneous policy impacts using causal machine learning: a case study of health insurance reform in Indonesia
Authors: Kreif Noemi , DiazOrdaz Karla , Moreno-Serra Rodrigo , Mirelman Andrew , Hidayat Taufik , Suhrcke Marc 
Published: November 2021

AbstractPolicymakers seeking to target health policies efficiently towards specific population groups need to know which individuals stand to benefit the most from each of these policies. While traditional approaches for subgroup analyses are constrained to only consider a small number of pre-defined subgroups, recently proposed causal machine learning (CML) approaches help explore treatment-effect heterogeneity in a more flexible yet principled way. Causal forests use a generalisation of the random forest algorithm to estimate heterogenous treatment effects both at the individual and the subgroup level. Our paper aims to explore this approach in the setting of health policy evaluation with strong observed confounding, applied specifically to the context of mothers’ health insurance enrolment in Indonesia. Comparing two health insurance schemes (subsidised and contributory) against no insurance, we find beneficial average impacts of enrolment in contributory health insurance on maternal health care utilisation and infant mortality, but no impact of subsidised health insurance. The causal forest algorithm identified significant heterogeneity in the impacts of contributory insurance, not just along socioeconomic variables that we pre-specified (indicating higher benefits for poorer, less educated, and rural women), but also according to some other characteristics not foreseen prior to the analysis, suggesting in particular important geographical impact heterogeneity. Our study demonstrates the power of CML approaches to uncover unexpected heterogeneity in policy impacts. The findings from our evaluation of past health insurance expansions can potentially guide the re-design of the eligibility criteria for subsidised health insurance in Indonesia.
CWENO Finite-Volume Interface Capturing Schemes for Multicomponent Flows Using Unstructured Meshes
Authors: Tsoutsanis Panagiotis , Adebayo Ebenezer Mayowa , Merino Adrian Carriba , Arjona Agustin Perez , Skote Martin 
Published: November 2021

AbstractIn this paper we extend the application of unstructured high-order finite-volume central-weighted essentially non-oscillatory (CWENO) schemes to multicomponent flows using the interface capturing paradigm. The developed method achieves high-order accurate solution in smooth regions, while providing oscillation free solutions at discontinuous regions. The schemes are inherently compact in the sense that the central stencils employed are as compact as possible, and that the directional stencils are reduced in size, therefore simplifying their implementation. Several parameters that influence the performance of the schemes are investigated, such as reconstruction variables and their reconstruction order. The performance of the schemes is assessed under a series of stringent test problems consisting of various combinations of gases and liquids, and compared against analytical solutions, computational and experimental results available in the literature. The results obtained demonstrate the robustness of the new schemes for several applications, as well as their limitations within the present interface-capturing implementation.