bluuebunny/crossref_metadata_2025_split huggingface dataset:
Strategy-dependent effects of working-memory limitations on human perceptual decision-making
Authors: Schapiro Kyra , Josić Krešimir , Kilpatrick Zachary P. , Gold Joshua I. 
Published: September 2021

AbstractDeliberative decisions based on an accumulation of evidence over time depend on working memory, and working memory has limitations, but how these limitations affect deliberative decision-making is not understood. We used human psychophysics to assess the impact of working-memory limitations on the fidelity of a continuous decision variable. Participants decided the average location of multiple visual targets. This computed, continuous decision variable degraded with time and capacity in a manner that depended critically on the strategy used to form the decision variable. This dependence reflected whether the decision variable was computed either: 1) immediately upon observing the evidence, and thus stored as a single value in memory; or 2) at the time of the report, and thus stored as multiple values in memory. These results provide important constraints on how the brain computes and maintains temporally dynamic decision variables.
Unearthing modes of climatic adaptation in underground storage organs across Liliales
Authors: Tribble Carrie M. , May Michael R. , Jackson-Gain Abigail , Zenil-Ferguson Rosana , Specht Chelsea D. , Rothfels Carl J. 
Published: September 2021

AbstractTesting adaptive hypotheses about how continuous traits evolve in association with developmentally-structured discrete traits, while accounting for the confounding influence of other, hidden, evolutionary forces, remains a challenge in evolutionary biology. For example, geophytes are herbaceous plants—with underground buds—that use underground storage organs (USOs) to survive extended periods of unfavorable conditions. Such plants have evolved multiple times independently across all major vascular plant lineages. Even within closely related lineages, however, geophytes show impressive variation in the morphological modifications and structures (i.e., “types” of USOs) that allow them to survive underground. Despite the developmental and structural complexity of USOs, the prevailing hypothesis is that they represent convergent evolutionary “solutions” to a common ecological problem, though some recent research has drawn this conclusion into question. We extend existing phylogenetic comparative methods to test for links between the hierarchical discrete morphological traits associated with USOs and adaptation to environmental variables, using a phylogeny of 621 species in Liliales. We found that plants with different USO type do not differ in climatic niche more than expected by chance, with the exception of root morphology, where modified roots are associated with lower temperature seasonality. These findings suggest that root tubers may reflect adaptations to different climatic conditions than those represented by other types of USOs. Thus, the tissue type and developmental origin of the USO structure may influence the way it mediates ecological relationships, which draws into question the appropriateness of ascribing broad ecological patterns uniformly across geophytic taxa. This work provides a new framework for testing adaptive hypotheses and for linking ecological patterns across morphologically varying taxa while accounting for developmental (non-independent) relationships in morphological data. [Macroevolution, geophytes, climatic niche evolution, imperfect correspondence]
MYB insufficiency disrupts proteostasis in hematopoietic stem cells leading to age-related neoplasia
Authors: Clarke M.L. , Lemma R.B. , Walton D.S. , Volpe G. , Noyvert B. , Gabrielsen O.S. , Frampton J. 
Published: September 2021

ABSTRACTThe Myb transcription factor plays critical roles in normal and malignant hematopoiesis. Acquired genetic dysregulation of Myb, which plays a central role in hematopoietic stem cell (HSC) gene regulation, is involved in the etiology of a number of leukemias. Also, inherited non-coding variants of the Myb gene are a factor in susceptibility to many hematological conditions, including myeloproliferative neoplasms (MPN), but the mechanisms by which variations in Myb levels predispose to disease, including age-dependency in disease occurrence, are completely unknown. Here, we address these key points by showing that Myb insufficiency in mice leads in later life to MPN, myelodysplasia, and leukemia, mirroring the age profile of equivalent human diseases. This age-dependence is intrinsic to HSC, involving progressive accumulation of subtle changes. Interestingly, and linking to previous studies showing the importance of proteostasis to the maintenance of normal HSC, we observed altered proteosomal activity in young Myb-insufficient mice and later elevated ribosome activity. We propose that these alterations collectively cause an imbalance in proteostasis, potentially creating a cellular milieu favoring disease initiation by driver mutations.
Genome size evolution in the beetle genusDiabrotica
Authors: Lata Dimpal , Coates Brad S , Walden Kimberly K O , Robertson Hugh M , Miller Nicholas J 
Published: September 2021

AbstractDiabrocite corn rootworms are one of the most economically significant pests of maize in the United States and Europe and an emerging model for insect-plant interactions. Genome sizes of several species in the genusDiabroticawere estimated using flow cytometry along with that ofAcalymma vittatumas an outgroup. Genome sizes ranged between 1.56 - 1.64 gigabase pairs (Gb) and between 2.26-2.59 Gb, respectively, for theDiabroticasubgroups fucata and virgifera; theAcalymma vittatumgenome size was around 1.65 Gb. This result indicated that a substantial increase in genome size occurred in the ancestor of the virgifera group. Further analysis of fucata group and virgifera group genome sequencing reads indicated that the genome size difference between theDiabroticasubgroups could be attributed to a higher content of transposable elements, mostly miniature inverted-transposable elements (MITEs) and gypsy-like long terminal repeat (LTR) retroelements.
Genome-wide analysis tracks the emergence of intraspecific polyploids inPhragmites australis
Authors: Wang Cui , Liu Lele , Yin Meiqi , Eller Franziska , Brix Hans , Wang Tong , Salojärvi Jarkko , Guo Weihua 
Published: September 2021

AbstractPolyploidization is a common event in plant evolution, and it plays an important role in plant speciation and adaptation. To address the role of polyploidization in grass diversification, we studiedPhragmites australis, a species with intraspecific variation of chromosome numbers ranging from 2n=36 to 144. A combined analysis of genome structure, phylogeny and population genetics were used to study the evolution ofP. australis. Whole-genome sequencing of three representative lineages revealed the allopolyploid origin of the species, with subgenome divergence dating back to approximately 29 million years ago, and the genomes showed hallmarks of relaxed selection associated with asexual propagation. Genome-wide analysis of 88 individuals from different populations around the world using restriction site associated DNA sequencing (RAD-seq) identified seven main intraspecific lineages with extensive genetic admixture. Each lineage was characterized by a distinct ploidy level, mostly tetraploid or octoploid, suggesting several polyploid events. Furthermore, we observed octoploid and hexaploid lineages at contact zones in Romania, Hungary and South Africa, suggestively due to genomic conflicts in allotetraploid parental lineages. Polyploidy may have evolved as a strategy to escape from the evolutionary dead-end of asexual propagation and the resulting decrease in genomic plasticity.
Metformin in nucleus accumbens core reduces cue-induced cocaine seeking in male and female rats
Authors: Chan Amy , Willard Alexis , Mulloy Sarah , Ibrahim Noor , Sciaccotta Allegra , Schonfeld Mark , Spencer Sade M 
Published: September 2021

AbstractThis study investigated the potential therapeutic effects of the FDA-approved drug metformin on cue-induced reinstatement of cocaine seeking. Metformin (dimethyl-biguanide) is a first-line treatment for type II diabetes that, among other mechanisms, is involved in the activation of adenosine monophosphate activated protein kinase (AMPK). Cocaine self-administration and extinction is associated with decreased levels of phosphorylated AMPK within the nucleus accumbens core (NAcore). Previously it was shown that increasing AMPK activity in the NAcore decreased cue-induced reinstatement of cocaine seeking. Decreasing AMPK activity produced the opposite effect. The goal of the present study was to determine if metformin in the NAcore reduces cue-induced cocaine seeking in adult male and female Sprague Dawley rats. Rats were trained to self-administer cocaine followed by extinction prior to cue-induced reinstatement trials. Metformin microinjected in the NAcore attenuated cue-induced reinstatement in male and female rats. Importantly, metformin’s effects on cocaine seeking were not due to a general depression of spontaneous locomotor activity. In female rats, metformin’s effects did generalize to a reduction in cue-induced reinstatement of sucrose seeking. These data support a potential role for metformin as a pharmacotherapy for cocaine use disorder, but warrant caution given the potential for metformin’s effects to generalize to a natural reward in female rats.
TTYH family members form tetrameric complexes within the cell membrane
Authors: Melvin Emelia , Shlush Elia , Giladi Moshe , Haitin Yoni 
Published: September 2021

AbstractThe conserved Tweety homolog (TTYH) family consists of three paralogs in vertebrates, displaying a ubiquitous expression pattern. Although considered as ion channels for almost two decades, recent structural and functional analyses refuted this role. Intriguingly, while all paralogs, studied following detergent solubilization, shared a dimeric stoichiometry, their spatial organization differed. Here, we determined the stoichiometry of intact mouse TTYH (mTTYH) complexes in cells. Using cross-linking and single-molecule fluorescence microscopy, we demonstrated that mTTYH1 and mTTYH3 form tetramers at the plasma membrane. Blue-native PAGE and fluorescence-detection size-exclusion chromatography analyses revealed that detergent solubilization results in the dissolution of tetramers into dimers, suggesting a dimer-of-dimers assembly mode. As cross-linking analysis of the soluble extracellular domains also showed tetrameric stoichiometry, we explored the effect of membrane solubilization and disulfide bridges integrity and established their contribution to tetramer stability. Future studies of the native tetrameric TTYH characterized here may illuminate their long-sought cellular function.
Molecular evolution and genetic analysis of Mesta yellow vein mosaic virus and associated betasatellites
Authors: Ahmed Rasel , Hasan Rajnee , Ullah Md. Wali , Ahmed Borhan 
Published: September 2021

AbstractMesta yellow vein mosaic disease (MYVMD), one of the major diseases circulating mesta growing regions of Indian sub-continent, is responsible for serious yield loss in mesta crops. A complex of monopartite begomovirus, Mesta yellow vein mosaic virus (MYVMV) and associated betasatellite, is reported in several studies as the causal agent of MYVMD. However, all-inclusive molecular evolutionary analysis of so far available MYVMVs and associated betasatellites disseminating in this region is still lacking. In this study, by estimating and analyzing various indexes of population genetics and evolutionary parameters, we discussed the sources of genetic variations, population dynamics and different forces acting on the evolution of MYVMVs and associated betasatellites. The study finds recombination as a vital force in the evolution and diversification of begomovirus complexes in different geographic locations however, betasatellites were found to be exposed to more diverse recombination events compared to MYVMVs. Indian isolates are reported to have high frequency of polymorphism in this study which suggests a balancing selection or expansion occurring in Indian populations of begomoviruses. Higher degree of genetic differentiation and lower rate of gene flow calculated between the viral populations of Bangladesh and Pakistan is justified by the relatively far geographical distance between these two countries. Although the study detects overall purifying selection, the degrees of constraints acting on individual gene tested are found different. Coat protein (AV1) is estimated with very high nucleotide substitution rate which is very likely to result from the strongest purifying selection pressure (dN/dS = 0.131) calculated in this study on coat protein. The findings of this study on different evolutionary forces that shape the emergence and diversification of MYVMVs and associated betasatellites may provide directions towards future evolutionary trend analysis and development of comprehensive disease control strategies for begomoviruses.
The potyviral protein 6K1 reduces plant protease activity during Turnip mosaic virus infection
Authors: Bera Sayanta , Arena Gabriella D. , Ray Swayamjit , Flannigan Sydney , Casteel Clare L 
Published: September 2021

SUMMARYPotyviral genomes encode just 11 major proteins and multifunctionality is associated to most of these proteins at different stages of virus life cycle. The potyviral protein 6K1 is required for potyvirus replication at the early stages of viral infection and may mediate cell-to-cell movement at later stages.Our study demonstrates that the 6K1 protein from Turnip mosaic virus (TuMV) reduces the abundance of transcripts related to jasmonic acid biosynthesis and transcripts that encode cysteine protease inhibitors when expressed in trans in Nicotiana benthamiana relative to controls. Furthermore, 6K1 stability increases when lipoxygenase and cysteine protease activity is inhibited chemically, linking a mechanism to the rapid turnover of 6K1 when expressed in trans.Using transient expression, we show 6K1 is degraded rapidly at early time points in the infection process, whereas at later stages of infection protease activity is reduced and 6K1 becomes more stable, resulting in higher TuMV accumulation in systemic leaves. There was no impact of 6K1 transient expression on TuMV accumulation in local leaves.Together, these results suggest a novel function for the TuMV 6K1 protein which has not been reported previously and enhances our understanding of the complex interactions occurring between plants and potyviruses.
A survey of complex trauma in families who have children and adults who have a learning disability and/or autism
Authors: Baker Peter , Cooper Vivien , Tsang Winnie , Garnett Isabelle , Blackman Noelle 
Published: September 2021

Purpose
There is an established literature supporting the idea that families who have children and adults who have a learning disability and/or autism have a greater vulnerability to mental health problems or poor psychological health. There are shortcomings in this literature in that there is a little consideration of the impact the families interaction with services has on their well-being. It is argued that complex post-traumatic stress disorder (CPTSD), with its focus on prolonged chronic exposure to trauma experiences and the recognition that this can occur in adulthood, may well be an appropriate framework to enable a better understanding of the experiences of families.

Design/methodology/approach
A total of 214 family members completed a co-produced online survey in relation to potential traumatic events, impacts and support.

Findings
The experiences of family carers of children and adults with a learning disability and/or who are autistic would appear to be multi-layered and complex, with many experiencing a wide range of traumatic events with the associated emotional and personal sequela. The reported responses are consistent with CPTSD with 10% of having received a diagnosis of PTSD. Their experience was that the system failed not only to provide support but also created additional trauma.

Practical implications
A trauma-informed approach needs to be adopted by agencies and professionals that serve families to ensure they understand their potential contribution to the trauma families experience.

Originality/value
To the best of the authors’ knowledge, this is the first study that has attempted to examine the experience of families using the framework of CPTSD.
One size does not fit all: the role of mentoring context in proactive individuals' scholarly impact
Authors: Ghitulescu Brenda E. , Khazanchi Shalini , Tang Zhi , Yu Yang 
Published: September 2021

PurposeMentoring relationships have been proposed as a potential intervention to alleviate gender disparities in scholarly output. Yet, previous research has not provided a systematic understanding of the relationship between mentoring and scholarly output. The authors propose that individuals with a proactive personality are especially suited to leverage mentoring relationships to enhance scholarly outcomes. Structural features of mentoring relationships – gender composition, mentor supervisory status, and mentoring relationship length – provide cues that encourage the expression of proactive personality and result in higher scholarly impact.Design/methodology/approachData were collected via surveys from faculty members in a US university and were matched with objective scholarly impact data. Hierarchical multiple regression analyses were used for hypothesis testing.FindingsThe impact of proactive personality on scholarly impact was more positive for women protégés with women mentors than for all other mentor-protégé pairings. Results also showed support for two hypothesized three-way interactions with mentor status and mentoring relationship length.Originality/valueThis research provides insights into the contexts where mentorship makes the most difference in protégés' scholarly achievement. Gender composition of mentoring dyads and mentor status are important boundary conditions that impact the effect of proactive personality on scholarly output.
Effects of weather conditions on concrete work task productivity – a questionnaire survey
Authors: Larsson Robert , Rudberg Martin 
Published: September 2021

Purpose
This paper aims to study the effects of different weather conditions on typical concrete work tasks’ productivity. Weather is one important factor that has a negative impact on construction productivity. Knowledge about how weather affects construction works is therefore important for the construction industry, e.g. during planning and execution of construction projects.

Design/methodology/approach
A questionnaire survey method is used involving means to perform pairwise comparisons of different weather factors according to the analytical hierarchical process (AHP). The survey also contains means to enable assessment of the loss in productivity for typical work tasks exposed to different weather types. The survey targets practitioners involved in Swedish concrete construction projects, and the results are compared with previous research findings.

Findings
The survey covers responses from 232 practitioners with long experience of concrete construction. The pairwise comparisons reveal that practitioners rank precipitation as the most important followed by wind and temperature. The loss in productivity varies significantly (from 0 to 100%) depending on the type of work and the type of weather factor considered. The results partly confirm findings reported in previous research but also reveal a more complex relationship between weather and productivity indicating several underlying influencing factors such as type of work, type of weather (e.g. rain or snow) and the intensity of each weather factor.

Originality/value
This paper presents new data about how 232 practitioners assess the effects of weather on construction productivity involving novel means to perform objective rankings such as the AHP methodology.
Smallholder farmers’ perception of climate change and adaptation strategy choices in Central Ethiopia
Authors: Addis Yonnas , Abirdew Solomon 
Published: September 2021

Purpose
Smallholder farmers have always been profoundly the first to be impacted by climate change, and therefore, farmers understanding of climate change and accessibility to alternative adaptation strategies are crucial for reducing the effect of climate change. The purpose of this study is to assess the perception of farmers to climate change, adaptation strategies and determinants of adaptation choice in central Ethiopia.

Design/methodology/approach
The study used data from randomly selected 240 farm households. Descriptive statistics were used to describe farmers’ perceptions of climate change and adaptation strategies. Also, a multivariate probit model was used to identify the major factors affecting farmers’ choice of adaptation strategies to climate change in central Ethiopia.

Findings
Smallholder farmers perceive climate change in the past two decades in response; the majority (91.47%) of farmers used adaptation options. Improved crop varieties and input intensity, crop diversification, planting date adjustment, soil and water conservation activities and changing of the crop type were used as adaptation options in the study area. A few of these strategies were significantly confirmed a complementary and supplementary relationship. The study identified sex, family size, agroecology, climate information, crop-fail history and formal extension service as significant determinants for farmers’ adaptation choices as these variables significantly affected more than two farmers’ adaptation strategies simultaneously.

Research limitations/implications
Farmers’ choice of adaptation was highly constrained by institutional factors and all these identified factors can be possibly addressed through a better institutional service provision system. It is, therefore, recommended that local administrators should explore the institutional service provision system for a better farm-level adaptation while considering demographic characteristics as well.

Originality/value
This study identified factors affecting farmers’ several adaptation strategies at a time and provides information for the policymaker to make cost-effective interventions for better farm-level adaptation practices.