bluuebunny/crossref_metadata_2025_split huggingface dataset:
A CRISPR-Cas9 screen reveals a role for WD repeat-containing protein 81 (WDR81) in the entry of late penetrating viruses
Authors: Snyder Anthony J. , Abad Andrew T. , Danthi Pranav 
Published: September 2021

ABSTRACTSuccessful initiation of infection by many different viruses requires their uptake into the endosomal compartment. While some viruses exit this compartment early, others must reach the degradative, acidic environment of the late endosome. Mammalian orthoreovirus (reovirus) is one such late penetrating virus. To identify host factors that are important for reovirus infection, we performed a CRISPR-Cas9 knockout (KO) screen that targets over 20,000 genes in fibroblasts derived from the embryos of C57/BL6 mice. We identified seven genes (WDR81, WDR91, RAB7, CCZ1, CTSL, GNPTAB, and SLC35A1) that were required for the induction of cell death by reovirus. Notably, CRISPR-mediated KO of WD repeat-containing protein 81 (WDR81) rendered cells resistant to reovirus infection. Susceptibility to reovirus infection was restored by complementing KO cells with human WDR81. Although the absence of WDR81 did not affect viral attachment efficiency or uptake into the endosomal compartments for initial disassembly, it delayed viral gene expression and diminished infectious virus production. Consistent with the role of WDR81 in impacting the maturation of endosomes, WDR81-deficiency led to the accumulation of reovirus particles in dead-end compartments. Though WDR81 was dispensable for infection by VSV (vesicular stomatitis virus), which exits the endosomal system at an early stage, it was required for VSV-EBO GP (VSV that expresses the Ebolavirus glycoprotein), which must reach the late endosome to initiate infection. These results reveal a broad, previously unappreciated role for WDR81 in promoting the replication of late penetrating viruses.AUTHOR SUMMARYViruses are obligate intracellular parasites that require the contributions of numerous host factors to complete the viral life cycle. Thus, the host-pathogen interaction can regulate cell death signaling and virus entry, replication, assembly, and egress. Functional genetic screens are useful tools to identify host factors that are important for establishing infection. Such information can also be used to understand cell biology. Notably, genome-scale CRISPR-Cas9 knockout screens are robust due to their specificity and the loss of host gene expression. Mammalian orthoreovirus (reovirus) is a tractable model system to investigate the pathogenesis of neurotropic and cardiotropic viruses. Using a CRISPR-Cas9 screen, we identified WD repeat-containing protein 81 (WDR81) as an essential host factor for reovirus infection of murine cells. Ablation of WDR81 blocked a late step in the viral entry pathway. Further, our work indicates that WDR81 is required for the entry of vesicular stomatitis virus that expresses the Ebolavirus glycoprotein.
Balance between protective and pathogenic immune responses to pneumonia in the neonatal lung enforced by gut microbiota
Authors: Stevens Joseph , Steinmeyer Shelby , Bonfield Madeline , Wang Timothy , Gray Jerilyn , Lewkowich Ian , Xu Yan , Du Yina , Guo Minzhe , Wynn James L. , Zacharias William , Salomonis Nathan , Miller Lisa , Chougnet Claire , O’Connor Dennis Hartigan , Deshmukh Hitesh 
Published: September 2021

AbstractWhile modern clinical practices like cesarean sections and perinatal antibiotics have improved infant survival, treatment with broad-spectrum antibiotics alters intestinal microbiota and causes dysbiosis. Infants exposed to perinatal antibiotics have an increased likelihood of life-threatening infections, including pneumonia. Here, we investigated how gut microbiota sculpt pulmonary immune responses, promoting recovery and resolution of infection in newborn rhesus macaques. Early-life antibiotic exposure, mirroring current clinical practices, interrupted the maturation of intestinal commensal bacteria and disrupted the developmental trajectory of the pulmonary immune system as assessed by single-cell proteomic and transcriptomic analyses of the pulmonary immune response. Early-life antibiotic exposure rendered newborn macaques susceptible to bacterial pneumonia, mediated by profound changes in neutrophil senescence, inflammatory signaling, and macrophage dysfunction. Pathogenic reprogramming of pulmonary immunity was reflected by a hyperinflammatory signature in all pulmonary immune cell subsets. Distinct patterns of immunoparalysis, including dysregulated antigen presentation in alveolar macrophages, impaired costimulatory function in T helper cells, and dysfunctional cytotoxic responses in natural killer (NK) cells, were coupled with a global loss of tissue-protective, homeostatic pathways in lungs of dysbiotic newborns. Fecal microbiota transfer corrected the broad immune maladaptations and protected against severe pneumonia. These data demonstrate the importance of intestinal microbiota in programming pulmonary immunity. Gut microbiota promote balance between pathways driving tissue repair and inflammatory responses, thereby leading to clinical recovery from infection in infants.One sentence summaryGut microbiota promote clinical recovery by reinforcing the balance between regenerative pathways driving tissue homeostasis and inflammatory responses limiting pathogens in infected neonatal lungs.
Using trait data improves correlation between environment and community data only if abundances are considered
Authors: Lengyel Attila , Barabás Sándor , Berki Boglárka , Csecserits Anikó , Gyalus Adrienn , Lhotsky Barbara , Ónodi Gábor , Rédei Tamás , Botta-Dukát Zoltán 
Published: September 2021

AbstractA straightforward way to explore variation between communities is to calculate dissimilarity indices and relate them with environmental and spatial variables. Communities are most often represented by the (relative) abundances of taxa they comprise; however, more recently, the distribution of traits of organisms included in the communities has been shown more strongly related to ecosystem properties. In this study, we test whether taxon- or trait-based dissimilarity is correlated more tightly with environmental difference and geographical distance and how the abundance scale influences this correlation. Our study system is grassland vegetation in Hungary, where we sampled vegetation plots spanning a long productivity gradient from open dry grasslands to marshes in three sites. We considered three traits for vascular plants: canopy height, specific leaf area and seed mass. We obtained field estimates of normalized vegetation difference index (NDVI) as proxy of productivity (water availability) for each plot. We calculated between-community dissimilarities using a taxon-based and a trait-based index, using raw and square-root transformed abundances and presence/absence data. We fitted distance-based redundancy analysis models with NDVI difference and geographical distance on the dissimilarity matrices and evaluated them using variance partitioning. Then, using the pooled data, we calculated non-metric multidimensional scaling ordinations (NMDS) from all types of dissimilarity matrices and made pairwise comparisons using Procrustes analysis. Data analysis was done separately for the three sites.We found that taxonomical dissimilarity matches environmental and spatial variables better when presence/absence data is used instead of abundance. This pattern was mainly determined by the increasing variation explained by space at the presence/absence scale. In contrast to this trend, with trait-based dissimilarity, accounting for abundance increased explained variation significantly due to the higher explanatory power of NDVI. With abundance data, considering traits improved environmental matching to a great extent in comparison with taxonomical information. However, with presence/absence data, traits brought no advantage over taxon-based dissimilarity in any respect. Changing the abundance scale caused larger difference between ordinations in the case of trait-based dissimilarity than with taxonomical dissimilarity.We conclude that considering relevant traits improves environmental matching only if abundances are also accounted for.Supporting informationAdditional graphs supporting the results are presented as appendix.Open researchData used in this research are publicly available from Dryad ###link to be supplied upon acceptance###
Avian haemosporidians (Plasmodium and Haemoproteus) status in selected bird groups (Old world Flycatchers, Warblers, Babblers, and Thrushes) of India and their phylogenetic relationships with other lineages of the world
Authors: Vipin  , Singh Ashutosh , Dixit Rajnikant , Tripathi Narinder Kumar , Singh Bhim , Sharma Vinita , Sharma Chandra Prakash , Mohan Dhananjai , Gupta Sandeep Kumar 
Published: September 2021

AbstractThe avian haemosporidians (Plasmodium and Haemoproteus) are widely distributed and may affect the host populations from body damage at individual level to the extinction of a population. The knowledge about their status may help in future avifauna conservation plans. Hence, we examined the avian haemosporidians status, in selected bird groups (Old world Flycatchers, Warblers, Babblers, and Thrushes) of India, and their phylogenetic relationships with other known lineages of the world. We used the common genetic marker (Cytochrome b gene fragment of 479 bp) with information on the geographic distribution of parasite and host species available at MalAvi database. The prevalence of avian haemosporidians, from northern, eastern, and southern parts of India and phylogenetic genetic analysis of lineages was carried out to know the genetic relatedness among them at local and world level. The MCC tree revealed six Haemosporidian lineages in which one was common (H_MSP01) and five were unique (H_CYOPOL01, H_CHD01, H_CYORUB01, H_EUMTHA01, and P_GEOCIT01). The avian host richness Index was 2.0852. 9.9%, prevalence of Haemosporidian infection was found in 111 DNA samples belonging to 6 host species. The Haemoproteus prevalence was found to be 9.0 % across five host species (Phylloscopus trochiloides, Cyornis poliogenys, C. hainanus dialilaemus, C. rubeculoides, Eumiyas thalassinus) and Plasmodium prevalence was 0.9% in Geokichla citrina. Spatial phylogeny at global level showed H_MSP01 lineage, found in different host species in India, was genetically related to H. pallidus lineages (COLL2 and PFC1) in parts of Africa, Europe, North America, Malaysia, and Philippines. The Plasmodium lineage (P_GEOCIT01) was related to PADOM16 in Egypt with poor sequence similarity (93.89%). The statistical analysis suggested that the haemosporidian’s host species distribution range was directly and significantly associated with the altitude, minimum temperature, and relative humidity. H_MSP01 distribution was in accordance with H. pallidus having a wide geographic and host range.
Synthesis, structure and in vitro biological properties of a new copper(II) complex with 4-{[3-(pyridin-2-yl)-1H-pyrazol-1-yl]methyl}benzoic acid
Authors: Li Xinhua , Niu Mengyuan , Wang Ai , Lu Liping , Englert Ulli , Feng Sisi , Zhang Lizhen , Yuan Caixia 
Published: September 2021

The new copper(II) complex dichloridobis(4-{[3-(pyridin-2-yl-κN)-1H-pyrazol-1-yl-κN
2]methyl}benzoic acid)copper(II) methanol sesquisolvate hemihydrate, [CuCl2
L
2]·1.5CH3OH·0.5H2O, (1), has been synthesized from CuCl2·2H2O and the ligand 4-{[3-(pyridin-2-yl)-1H-pyrazol-1-yl]methyl}benzoic acid (L, C15H11N3O2). The complex was characterized by elemental analysis, Fourier transform IR spectroscopy, electrospray ionization mass spectrometry and single-crystal X-ray diffraction. Two chloride ligands and two bidentate L ligands coordinate the CuII centre in 1 in a Jahn–Teller-distorted octahedral geometry of rather unusual configuration: a chloride substituent and a pyrazole N atom of an N,N′-chelating ligand occupy the more distant axial positions. Classical O—H...O hydrogen bonds and O—H...Cl interactions link neighbouring complex molecules and cocrystallized methanol molecules into chains that propagate parallel to the b direction. The title compound shows intriguing bioactivity: the effects of 1 on the enzymatic activity of protein tyrosine phosphatase 1B (PTP1B) and on the viability of human breast cancer cells of cell line MCF7 were evaluated. Complex 1, with an IC50 value of 0.51 µM, can efficiently inhibit PTP1B activity. An enzyme kinetic assay suggests that 1 inhibits PTP1B in a noncompetitive manner. A fluorescence titration assay indicates that 1 has a strong affinity for PTP1B, with a binding constant of 4.39 × 106
M
−1. Complex 1 may also effectively decrease the viability of MCF7 cells in an extent comparable to that of cisplatin (IC50 = 6.3 µM). The new copper complex therefore represents a promising PTP1B inhibitor and an efficient antiproliferation reagent against MCF7 cells.
Requirements and process analysis for ports and waterways openBIM ISO standards development
Authors: Bradley Alex , Li Haijiang , Qin Honglei , Xi Wen , Peel Daniel , Nisbet Nicholas 
Published: September 2021

Purpose
Defining building information modelling (BIM) standards for the infrastructure domain is a central issue to the successful implementation of BIM in civil engineering domains. To this end, this paper aims to present a requirements and process analysis for the ports and waterways domain to address the lack of BIM standards development, using the information delivery manual (IDM) approach and the ethos of openBIM standards.

Design/methodology/approach
This research uses the IDM approach. This involves the definition of use cases, process maps, exchange scenarios and subsequent exchange requirements. All these developments were sourced and validated by a series of international industry consultations.

Findings
The paper identifies 30 domain relevant use cases collated from existing sources and new cases. An overview and detailed ports and waterways process map (defining actors, activities and data exchanges). The process maps highlighted 38 exchange scenarios between various activities. Various exchange requirements were defined and are discussed in the context of the required information exchange model and the extensions required to fulfil the needs of the domain. The analysis provides the core information for the next steps of development for a substantial extension to the Industry Foundation Classes and the supporting data dictionary standards.

Research limitations/implications
Because of the international scope of the research, the outcomes can be applied by any stakeholders in the domain of ports and waterways. Therefore, some variation is expected at a national and organizational level. This research has the potential to accelerate the adoption of openBIM standards within the ports and waterways domain leading to increases in efficiency, collaborative working.

Originality/value
This paper reviews the requirements of an identified gap in the provision of openBIM standards relevant and applicable to the domain of ports and waterways.
The direct and interactive effects of retail community engagement
Authors: Lund Donald J. , Hansen John D. , Robicheaux Robert A. , Cid Oreja Clara 
Published: September 2021

PurposeThis paper aims to examine the direct and interactive effects of community engagement and economic value on customers’ trust in, and commitment to, the retailer. This paper also examines the extent to which these variables, in turn, drive desirable behavioral outcomes in the form of positive word of mouth communications and share of customer.Design/methodology/approachStudy results are derived from a cross-sectional survey of 1,757 respondents.FindingsThe authors find that retailer community engagement positively impacts word of mouth and share of customer indirectly through commitment and trust. As hypothesized, the results support a suppressing interaction between community engagement and economic value on trust. Contrary to the hypothesis, the interactive effects on commitment are positive.Research limitations/implicationsThe study uses cross-sectional, single-sourced data. Incorporating secondary data or using experiments would reinforce these findings. This research is limited to local community engagement, future studies could broaden the focus to strategies that benefit communities outside the local area.Practical implicationsStudy results indicate that managers can indeed build stronger customer relationships through community engagement as customers are more apt to be trusting of and committed to retailers perceived to be more actively engaged in the community. These findings are particularly important considering that community engagement is typically less expensive than other marketing strategies. Community investments are inexpensive initiatives that retailers can leverage to generate a big impact in the hearts and minds of their customers.Originality/valueWhile it seems logical to assume that community engagement will benefit retailers in the form of stronger customer relationships, the authors empirically validate this assumption. The finding that community engagement simultaneously serves as both an antecedent and moderator is novel, albeit counterintuitive in the sense that the variable negatively moderates the economic value-trust relationship while positively moderating the economic value-commitment relationship. Taken in their totality, these findings indicate that community engagement serves to simultaneously drive stronger customer relationships while also differentially affecting the way customers arrive at their assessments.
Spectators' perceived social benefits, subjective well-being and support intention: non-mega-parasporting events' educational perspective
Authors: Yamashita Rei , Muneda Masaya 
Published: September 2021

PurposeIt is crucial to create an inclusive society through sport. Many researchers have focused on the social benefits of hosting events and identified the necessity of revealing the impact of parasport. There is inadequate knowledge of what local residents perceive from spectating non-mega-parasporting events. Therefore, this research aimed to reveal the influence of spectators' perceived social benefits, subjective well-being, and support for parasport events.Design/methodology/approachA survey was conducted with questionnaires distributed to spectators of two wheelchair basketball tournaments. Of the 2,100 questionnaires distributed, 1,996 were returned; of these, 1,544 were rejected because of not having local resident status. The final response rate of useable questionnaires was 22.6% (n = 452).FindingsBoth community benefits and cultural/educational benefits positively affected subjective well-being, which also significantly affected support for the event. Further, there was no significant moderating effect of having family members or close friends with disability.Practical implicationsThe results indicate that sporting event managers can collaborate with local educational boards and use sporting events as educational material for children to raise next-generation, highly diversity-minded leaders from the local area.Originality/valueThis study revealed that parasporting events have educational value. Additionally, athletes with disabilities inspire spectators, which changes the perception of their educational impacts.
Conflicts, trust toward the acquirer from emerging economies and post-acquisition cooperation intention
Authors: Zhang Xun , Wu Jun , Zhang Ning , Xu Biao 
Published: September 2021

PurposeThe purpose of this paper is to examine the impact of inter-group conflicts on the trust toward the acquirer and then further on cooperation intention after acquisitions in cross-border mergers and acquisitions (M&As), in the lens of the social classification theory. Two types of conflicts (realistic conflicts and symbolic conflicts) and a mediating mechanism (trust toward the acquirer) exhibit different effects on cooperation intention. The research further examines two moderating coping strategies (localizing management and assigning trustworthy expatriate managers) and tests their effectiveness in promoting trust toward the acquirer and increasing cooperation intention in cross-border M&As.Design/methodology/approachThe research hypotheses were empirically tested in the context of post-acquisition of Chinese companies' cross-border M&As. In total, 600 questionnaires were provided to the research participants of 37 acquired firms/units from advanced economies of 12 Chinese companies, and 209 valid questionnaires were collected (the response rate is 34.83%). Confirmatory factor analysis was conducted to verify data validity and hierarchical multiple regression analyses were employed to test relational and moderating effects.FindingsThis research demonstrates that both realistic and symbolic conflicts can reduce the intention to cooperate, but the latter does not have a significant influence. The results also uncover that whether employees from acquired firms trust in their acquirers mediates the relationship between realistic conflicts and cooperation intention. Moreover, management localization (a measurement of whether local/original managers are retained with a high degree of freedom and autonomy after M&As) and trustworthiness of expatriate managers (a measurement of whether the assigned expatriate managers is trustworthy) positively moderate the relation between realistic conflicts and trust toward the acquirer. In addition, trust toward the acquirer mediates the interaction of realistic conflicts and management localization on the cooperation intention of the acquirers' employees.Originality/valueThis study examines how inter-organizational conflicts influence trust toward the acquirer and then cooperation intentions in the context of Chinese companies' M&A behavior of targets from developed countries and gain supportive evidence, which enriches the literature on the management of international M&As. By introducing two management localization and trustworthiness of expatriate managers into the model, the research deepens our knowledge of how to build trust toward the acquirer in cross-border M&As.
Managerial ability, earnings quality and ISIS: evidence from Iraq
Authors: Bazrafshan Ameneh , Makarem Naser , Hesarzadeh Reza , SalmanAbbood Wafaa 
Published: September 2021

PurposeThis study investigates the association between managerial ability and earnings quality in firms listed on the Iraq Stock Exchange and how the emergence of the Islamic State of Iraq and Syria (ISIS) influences the association.Design/methodology/approachThis study uses a sample of firms listed on the Iraq Stock Exchange over the period 2012–2018. Managerial ability is quantified using data envelopment analysis, and earnings quality is measured by earnings restatement, earnings persistence, accruals quality and earnings response coefficient. Panel regression analysis is used to examine the research hypotheses.FindingsThe findings indicate that managerial ability positively affects earnings quality of Iraqi firms and that ISIS weakens the relationship between managerial ability and earnings quality. These findings are robust to the alternative measures of managerial ability, as well as to various approaches used to address endogeneity including propensity-score matching and a difference-in-differences analysis.Originality/valueThis study provides insight into the impact of managerial ability on earnings quality in an under-studied emerging market. Furthermore, this study broadens the existing literature about the financial consequences of a modern terrorist group, ISIS.