bluuebunny/crossref_metadata_2025_split huggingface dataset:
Standard Peripheral Blood Mononuclear Cell Cryopreservation Selectively Decreases Detection of Nine Clinically Relevant T Cell Markers
Authors: Capelle Christophe M , Ciré Séverine , Ammerlaan Wim , Konstantinou Maria , Balling Rudi , Betsou Fay , Cosma Antonio , Ollert Markus , Hefeng Feng Q 
Published: August 2021

Abstract
Biobanking is an operational component of various epidemiological studies and clinical trials. Although peripheral blood is routinely acquired and stored in biobanks, the effects of specimen processing on cell composition and clinically relevant functional markers of T cells still require a systematic evaluation. In this study, we assessed 25 relevant T cell markers in human PBMCs and showed that the detection of nine membrane markers (e.g., PD-1, CTLA4, KLRG1, CD25, CD122, CD127, CCR7, and others reflecting exhaustion, senescence, and other functions) was reduced among at least one T cell subset following standard processing, although the frequency of CD4, CD8, and regulatory T cells was unaffected. Nevertheless, a 6-mo-long cryopreservation did not impair the percentages of cells expressing many other membrane and all the eight tested intracellular lineage or functional T cell markers. Our findings uncover that several clinically relevant markers are particularly affected by processing and the interpretation of those results in clinical trials and translational research should be done with caution.
Visceral Adiposity Index and Lipid Accumulation Product as diagnostic markers of Metabolic Syndrome in South Indians with Polycystic Ovary Syndrome
Authors: Naghshband Zeinab , Kumar Lakshmi , Mandappa Sonia , Niranjana Murthy Ashitha S. , Malini Suttur S. 
Published: August 2021

Background:
Cardiovascular disease (CVD) is one of the debilitating consequences of polycystic ovary syndrome (PCOS). Early diagnosis of metabolic syndrome (MetS) with a simple but accurate method can reduce the risk of progression to CVD in PCOS.

Aims:
This study aimed to determine the accuracy of various anthropometric indices and lipid accumulation product (LAP), in assessing the risk of MetS in PCOS.

Settings and Design:
This is a cross-sectional study including 150 PCOS women and 100 control subjects.

Materials and Methods:
Anthropometric parameters were measured and calculated. Lipid profile, fasting plasma glucose (FPG), and insulin were estimated. MetS was detected according to the International Diabetes Federation criteria.

Statistical Analysis:
Logistic regression and receiver operating characteristic curve analysis were applied to determine the potential association of anthropometric indices such as body mass index, waist circumference (WC), waist-to-hip ratio, waist-to-height ratio, conicity index (CI), visceral adiposity index (VAI), abdominal volume index (AVI), body adiposity index (BAI), and a body shape index (ABSI) and LAP with MetS.

Results:
In our study of PCOS women of the south Indian population, the prevalence of MetS was 59.3%, which was higher than other populations and the cutoff values of VAI and LAP were 6.05 and 53, respectively. VAI showed the strongest association with MetS, followed by diastolic blood pressure BP, FPG, and LAP.

Conclusions:
We recommend VAI and LAP as new indices for MetS diagnosis. As these indices exhibit population specificity, it is imperative that independent cutoffs are determined for every demographic population.
Antibacterial Activity of Flavonoids from Ethyl Acetate Extract of Milk Banana Peel (Musa x paradisiaca L.)
Authors: Rita Wiwik Susanah , Asih Ida Ayu Raka Astiti , Swantara I Made Dira , Damayanti Ni Luh Yuli 
Published: August 2021

Infectious diseases caused by Staphylococcus aureus and Escherichia coli can be treated by milk banana peels (Musa × paradisiaca L.). This study aims to reveal the antibacterial activity of milk banana peel ethyl acetate extract against Staphylococcus aureus and Escherichia coli, and to identify their active compounds. Extraction was conducted by maceration at room temperature, followed by partition. Separation was carried out by column chromatography with mobile phase of chloroform: ethyl acetate (7.5:2.5) and stationary phase of silica gel 60. Antibacterial assay was performed by well diffusion method and identification of active compounds was analyzed by UV-Vis spectrophotometry and LC-MS/MS. Extraction of the peel powder produced methanol extract, partition of the methanol extract resulted n-hexane, ethyl acetate, n-butanol, and water fractions. Antibacterial activity assay shows that ethyl acetate extract was the most active to inhibit the growth of both bacteria. Separation with column chromatography resulted 5 fractions. Identification of the most active fraction with UV-Vis showed that the isolate gave maximum absorption at λ 339.00 nm (band I) and λ 262.00 nm (band II) which were thought to be flavonol (3-OH substituted). Analysis with LC-MS/MS shows that the most active fraction contained 3-methacrylate flavonols and 3-(furan- 2yl) acrylate flavonols.
Microdose Flare-up Gonadotropin-releasing Hormone (GnRH) Agonist Protocol and GnRH Antagonist Protocol: Effects on In-vitro Fertilization in Patients with Poor-responder Diagnosis According to Bologna Criteria
Authors: Görgülü Gökşen , Çakır Köle Merve , Aldemir Oya , Köle Emre , Dilbaz Serdar 
Published: August 2021

Objective: The aim of the study was to evaluate microdose flare-up Gonadotropin-Releasing Hormone (GnRH) agonist protocol and GnRH antagonist protocol with respect to their effects on in-vitro fertilization (IVF) results in patients with poor ovarian response according to the Bologna Criteria.
Material Methods: This was a retrospective cohort study conducted in the Assisted Reproduction clinic of University of Health Sciences, Etlik Zübeyde Hanım Gynaecology Training and Research Hospital. A total of 645 patients who had been diagnosed as poor responders in our clinic, between 2007 and 2018, and received treatment with either microdose flare-up GnRH agonist protocol (n=250, 38.8%) or GnRH antagonist protocol (n=395, 61.2%), were included in the study.
Results: The mean age of the study group was 34.5±5.5 years. Comparisons showed that IVF cycle cancellation frequency (p<0.01), third day estradiol level (p=0.04) and third day follicle stimulating hormone level (p<0.01) were significantly greater in patients who underwent the microdose flare-up protocol. In the GnRH antagonist group, the number of surviving children (p=0.01), antral follicle count (p<0.01), follicle count on day of human chorionic gonadotropin (hCG) administration (p<0.01), endometrial thickness on hCG day (p<0.01), number of oocytes collected (p<0.01), mature oocyte count (p<0.01), embryo transfer number (p<0.01) were higher compared to the microdose flare-up protocol group. The two groups were similar in terms of clinical pregnancy rate.
Conclusions: In terms of clinical pregnancy rate, the IVF results of microdose flare-up and GnRH antagonist protocols are similar. Further studies are needed to reach more comprehensive results on the subject.
Utility of screening chest radiographs in patients with asymptomatic and mildly symptomatic COVID-19 at a field hospital in Samut Sakhon, Thailand
Authors: Trimankha Pilawan , Lakkana Jirapong Lakkana , Rungsin Runnaya , Autravisittikul Orawan , Deesuwan Pariyanoot Deesuwan, M.D. , Mekavuthikul Yasinee , Chodpanich Anocha 
Published: August 2021

Background: In a new episode of the COVID -19 pandemic in Thailand during the beginning of 2021, cases in Samut Sakhon Province mainly occurred in foreign workers and were mostly asymptomatic or had mild disease. To prevent overwhelming the local hospital, a field hospital was established which used chest radiography as one of screening tools for triaging patients.
Objective: To determine the clinical utility of chest radiographs as a screening tool for COVID-19 patients who were asymptomatic or mildly symptomatic.
Materials and Methods: Six hundred nineteen patients with COVID -19 (confirmed by reverse transcriptase-polymerase chain reaction) were registered at the field hospital at Samut Sakhon provincial sport stadium during 5-8 January 2021 and had chest radiographs taken. The image readings were based on the consensus of two radiologists and a final decision was made by a third radiologist if the first two did not agree. Findings on chest radiographs and clinical outcomes were evaluated.
Results: The study included 619 radiographs; 328/619 (53%) men and 291/619 (47%) women had a mean age of 33.3+/- 9.7 (range, 5-64) years. There was mild disease in 13/619, and asymptomatic infections in 606/619. Chest radiographs were normal in 568 (91.7%) and abnormal in 51 (8.3%) patients; typical findings of COVID-19 were seen in 3 (0.5%) patients. Other abnormal findings were found in 23 (3.8%) patients such as active tuberculosis in 6 (1.0%). Four patients were transferred to the hospital, one of whom required supplemental oxygen.
Conclusion: Combined chest radiographic and clinical information allows better decisions regarding hospital transfers of asymptomatic and mildly symptomatic COVID-19 patients at a field hospital.
Educación a distancia y creación de espacios de interacción
Authors: Bustamante Suleima , Torres Yury 
Published: August 2021

El presente ensayo se sustenta en el análisis sobre los cambios experimentados en la educación a distancia (EAD) y en la propia educación tradicional, con la incorporación en la práctica pedagógica de las tecnologías de información y comunicación (TIC) y el surgimiento de paradigmas a partir de la interacción de los distintos actores del proceso enseñanza-aprendizaje a través de: e-learding, b-learding, chat. La discusión gira en torno a las tecnologías como herramientas para innovar en educación, conforme a una metodología documental, crítico-reflexiva, centrada en la construcción del conocimiento, para arribar a evidencias que orientan la estructura académica - científico- tecnológica de las instituciones de educación superior, reflejadas en la innovación curricular, adaptación de planes y programas afianzados en la modalidad de EAD y el espacio virtual con el uso de las TIC. Finalmente se establece que para generar un impacto de las TIC en la configuración de nuevos modelos de enseñanza, se requiere de una visión integradora de políticas educativas, organizacionales, recursos tecnológicos y actores involucrados, ganados para el trabajo compartido.
Perspectivas teóricas y epistemológicas de la investigación cualitativa en educación: ideas básicas para su comprensión
Authors: Rojas Torres Ana Cecilia , Rojas de Chirinos Blanca 
Published: August 2021

En el campo de las Ciencias Sociales, la investigación se ha convertido en una actividad importante y necesaria en educación, dando origen a una disciplina académica denominada investigación educativa, categoría conceptual amplia que en los últimos tiempos ha adoptado nuevos significados, a la par que han aparecido nuevos enfoques y modos de entender el hecho educativo, convirtiéndose la investigación cualitativa en una alternativa que permite comprender cómo los actores sociales experimentan, perciben, crean, modifican e interpretan la realidad educativa. En este sentido surge la iniciativa de presentar este ensayo con la finalidad proporcionarle al docente conocimientos básicos sobre las perspectivas teóricas y epistemológicas de la metodología cualitativa que le ayude a comprender el objetivo que se propone cada método, en el marco del cuerpo teórico de este tipo de investigación.
El aprendizaje cooperativo desde el fundamento de la teoría vygotskyana
Authors: Andrade Francisco , Sarcos Dilia 
Published: August 2021

En este estudio se analiza el enfoque aprendizaje cooperativo (AC) desde la teoría vygotskyana (TV). Es una investigación documental, sustentada en estudios previos y datos conseguidos en diversas fuentes impresas. El estudio muestra que el AC tiene una relación intrínseca con la perspectiva de la TV, dado que ambas centran su atención en la mediación grupal, la interdependencia, la interacción social y multidirección de la acción. Como conclusión se tiene que el AC representa un enfoque que valida un proceso de construcción del aprendizaje basado en la interacción grupal, al llegar a formar relaciones de alta calidad y habilidades necesarias para desarrollar una tarea con excelencia. En igual sentido se tiene, que los postulados de la teoría histórico socio cultural, relacionados con los procesos superiores y la zona de desarrollo próximo, dan explicación a la internalización de la información mediante la interacción social, en el cual puede sustentarse el AC.
EXPLICIT FORM FOR THE FIRST INTEGRAL AND LIMIT CYCLES OF A CLASS OF PLANAR KOLMOGOROV SYSTEMS
Authors: Boukoucha Rachid 
Published: August 2021

In this paper we characterize the integrability and the non-existence of limit cycles of Kolmogorov systems of the form
\begin{equation*} \left\{ \begin{array}{l} x^{\prime }=x\left( R\left( x,y\right) \exp \left( \dfrac{A\left( x,y\right) }{B\left( x,y\right) }\right) +P\left( x,y\right) \exp \left( \dfrac{C\left( x,y\right) }{D\left( x,y\right) }\right) \right) , \\ \\ y^{\prime }=y\left( R\left( x,y\right) \exp \left( \dfrac{A\left( x,y\right) }{B\left( x,y\right) }\right) +Q\left( x,y\right) \exp \left( \dfrac{V\left( x,y\right) }{W\left( x,y\right) }\right) \right) , \end{array} \right. \end{equation*}
where $A\left( x,y\right)$, $B\left( x,y\right)$, $C\left( x,y\right)$, $D\left( x,y\right)$, $P\left( x,y\right)$, $Q\left( x,y\right)$, $R\left(x,y\right)$, $V\left( x,y\right)$, $W\left( x,y\right)$ are homogeneous polynomials of degree $a$, $a$, $b$, $b$, $n$, $n$, $m$, $c$, $c$, respectively. Concrete example exhibiting the applicability of our result is introduced.
Primary squamous cell carcinoma of the sigmoid colon: a case report and literature review
Authors: Pascacio Fiori Martín , Alférez Andía Jéssica , Kapsoli Sánchez María , Benites Goñi Harold 
Published: August 2021

Primary squamous cell carcinoma of the colon is extremely rare. The etiology is poorly understood, and currently, there are different hypotheses about the origin of this malignant neoplasm. Here, we r 87-year-old male with a moderately-differentiated nonkeratinizing squamous cell carcinoma of the colon.
Virtual Hearing Aid Care – Clinical Practice Guidance Document
Authors: Glista Danielle , O’Hagan Robin , DiFabio Danielle , Moodie Sheila , Muñoz Karen , Joseph Keiran , Brown Christine , Curca Ioan , Meston Christine , Richert Frances , Bagatto Marlene , Pfingstgraef David 
Published: August 2021

This document was informed by literature reviews conducted in accordance with the Joanna Briggs Institute’s guide to evidence synthesis (Aromataris & Munn, 2017; https://joannabriggs.org) and includes evidence related to client candidacy, delivery models, modalities of delivery, and outcomes of virtual hearing aid fitting and management. This document provides clinical practice guidance for virtual hearing aid fitting and management processes and technological requirements in the delivery of such services (herein referred to as virtual hearing aid care). Virtual hearing aid care can include services delivered directly to a client by a provider or using facilitator-supported services and specialized equipment, depending on client factors, type of care, and the timepoint in the care process (e.g., initial versus follow-up appointments). This document will address virtual care including the following types of hearing aid care: o	Programming o	Verification o	Validation o	Management (counselling and education) Currently, virtual hearing aid care is better suited to follow-up appointments
PENGARUH PARTISIPASI ANGGARAN, KETEPATAN ANGGARAN TERHADAP SENJANGAN ANGGARAN DENGAN KOMITMEN ORGANISASI SEBAGAI VARIABEL MODERASI (STUDI PADA PEMERINTAH KABUPATEN JAYAPURA)
Authors: Kalembang Meise , Allo Layuk Paulus K. , Falah Syaikhul 
Published: August 2021

The purpose of this research is to understand the influence of the participation and the budget acuracy against budget gap with a current commitment of organization as moderation variables. This kind of research is quantitative research by the use of the primary data. The research population is civil servants of SKPD in Jayapura regency. The amount of sample was 105. Method used in the study was moderated regression analysis.
The results of this study found that the budgeting participation has a positive and significant impact on budget gap, while the budget accuracy can not have positive and significant impact on budget gap. Organization commitment as moderate variable cannot moderating budgeting participation and the budget accuracy to budget gap in Jayapura regency.

Keywords: Budget participation, budget accuracy, budget gap, organization commitment.
Stress Echocardiography with Adenosinethreephosphate and Atrial Pacing in Diagnosis of Late Coronary Shunts Stenosis
Authors: Talanova A. V. , Lezhnev D. A. , Mikheev N. N. 
Published: August 2021

The aim of the study was to evaluate diagnostic accuracy of stress echocardiography (stress echoCG) with combination of high doze АТР and atrial pacing in diagnosis of late coronary shunts stenosis.48 men aged from 45 to 64 (48,4 ± 1,6 years), 36 patients undergone mammarocoronary shunting, 12 patients undergone auto venous shunting. Stress echoCG with combination of high doze ATP and atrial pacing suggested shunts lesions in 44 patients, in 8 cases previously intact coronary arteries, approved with coronary angiography. Coronary angiographies reviled no coronary lesions in 24 patients > 70 % shunts restenosis, in 22 coronary stenosis from 50 up to 70 %. Accuracy, sensitivity and specificity of stress- EchoCG with combination of stress-agents in coronary shunts restenosis, as well as stenosis of previously intact coronary arteries were 100 %.
Changes in Hemodynamic Parameters in Various Morphological Changes in the Kidney Transplant in Children
Authors: Pykov M. I. , Ektov D. B. 
Published: August 2021

Ultrasound scanning is one of the main methods of instrumental examination of patients after allogenic transplantation of kidney. The main reasons of dysfunction of the kidney transplant in long posttransplantation term are acute or chronic rejection, as well as acute and chronic nephrotoxicity of calcineurin inhibitors. The study includes an analysis of the medical history of 130 children with end-stage chronic renal failure who underwent 130 allogeneic kidney transplants from a posthumous donor. There were analyzed the results of 231 percutaneous puncture biopsies and ultrasound studies of renal transplants. The analyzed data of morphological studies are divided into 4 groups:1st group — there are no morphological changes affecting graft function;2nd group — morphological signs of calcineurin toxicity;3rd group — borderline damage of the graft;4th group — acute and chronic graft rejection;5th group — non-specific tubulointerstitial sclerosis and tubular atrophy.Indices of resistance and pulsation measured at the level of the renal artery and interlobular arteries tended to decrease in the kidneys with immunological influence and remained stable in other morphological groups. This means that, the higher the degree of hyalinosis of the arteries, the lower the indices of resistance and pulsation indices measured at the level of the common renal artery and interlobular arteries. The revealed tendency of a decrease in the values of the resistance index and the pulsation index at the level of the common renal artery of the interlobular arteries can be considered as one of the initial ultrasound signs that allow to speak of a decrease in the elasticity of the vascular wall. Normal indices of renal hemodynamics do not exclude the presence of pathological processes leading to graft dysfunction.
Формы организации историко-краеведческой деятельности студенчества в системе вуза с начала 1930-х до середины 1950-х гг. (на материале Ворошиловградской области УССР)
Authors: Анпилогова Татьяна Юрьевна 
Published: August 2021

На основе анализа широкой источниковой базы автор выделяет формы историко-краеведческой деятельности студенческой молодежи советских республик в 1930–1950-е гг. Охарактеризован процесс организации таких видов работы студентов, как археологические исследования, научно-исследовательская работа в системе научных студенческих обществ, историко-краеведческая работа в рамках прохождения производственной педагогической практики в школе студентами педвузов, выполнение дипломных работ на краеведческую тематику. Освещена проблема использования элементов историко-краеведческой работы студенчества в ходе общественно-полезной деятельности. В основе авторской позиции лежит утверждение о том, что возрождение некоторых применяемых в советской педагогической практике форм организации историко-краеведческой деятельности студенчества даст возможность обеспечить формирование ряда важных универсальных компетенций выпускников современных вузов.
Evaluation du potentiel des series d’images multi-temporelles optique et radar des satellites SENTINEL 1 & 2 pour le suivi d’une zone côtière en contexte tropical: cas de l’estuaire du Cameroun pour la période 2015-2020
Authors: Njutapvoui Nourdi , Onguene Raphael , Rudant Jean Paul 
Published: August 2021

Ce travail porte sur l’évaluation du potentiel des images multi-temporelles et multi-capteurs (optique et radar) des satellites Sentinel 1 et 2 pour la cartographie de l’occupation du sol et le suivi de l’évolution du trait de côte dans un écosystème tropical sur la période 2015 à 2020. La zone d’étude choisie est l’Estuaire du Cameroun. Cette zone représente un milieu de transition écologique majeur dans la sous-région avec la présence de réserves naturelles protégées (mangroves, forêt dense, zones humides) mais aussi une forte activité anthropique (constructions, agriculture, forêt dégradée). L’approche méthodologique a consisté en une chaine de prétraitements et d’analyses visuelles d’images, suivie d’une combinaison des bandes de chaque capteur, d’une classification supervisée Random Forest pour ébaucher une cartographie de l’occupation du sol et enfin une numérisation du trait de côte. Globalement, les résultats montrent que la classification, avec les images Sentinel 2 en utilisant 10 bandes et en ajoutant 4 indices de végétation, s’avère légèrement plus précise (95.75%) que celle issue des 13 bandes initiales (91.78%). La classification avec les seules images Sentinel 1A double polarisation (VV, VH) conduit à une précision de 78.44%. La combinaison des bandes Sentinel 2A (10 bandes et 4 indices) et Sentinel 1 améliore les résultats et conduit à une précision de 98.76%. Nos résultats montrent aussi que l’utilisation des séries chronologiques d’images multi-temporelles améliore considérablement la précision de classification par rapport à l’usage d’une seule image (mono-date), et cela pour les deux capteurs, soit un gain supplémentaire de 13% et 10% respectivement pour Sentinel-2 et Sentinel-1. Néanmoins ce gain reste faible pour les classes temporellement stables. Les résultats d’analyse de l’évolution du trait de côte montrent que l’estuaire du Cameroun est perturbé selon différents niveaux d’érosion (Cap Cameroun, Partie Nord île Manoka, embouchure de la Sanaga), et d’accrétion (Limbé, et Partie Sud île Manoka) et aussi par de faible variations internes. La fusion des données de télédétection optique et radar dans la discrimination des classes d’occupation du sol, a permis de montrer que les zones de constructions sont les plus vulnérables à l’érosion côtière. Par contre, la présence de la végétation (mangrove, forêt) stabilise et protège la côte d’éventuels risques de cette nature.