bluuebunny/crossref_metadata_2025_split huggingface dataset:
TRIM22 Activates MAPK Signaling by Regulating the Transcription of SPHK2 and Accelerating the Degradation of Raf-1 in Glioblastoma
Authors: Fei Xiaowei , Dou Ya-nan , Sun Kai , Wei Jialiang , Guo Qingdong , Wang Li , Wu Xiuquan , Lv Weihao , Fei Zhou 
Published: November 2021

Abstract
Background

Tripartite motif (TRIM) 22 and mitogen-activated protein kinase (MAPK) signaling pathways play a critical role in tumor growth and therapeutic resistance of glioblastoma (GBM) respectively. However, the molecular mechanism between TRIM22 and MAPK signaling remains to be clarified.
Methods

We constructed TRIM22 knockout cell lines for molecular biology experiments, detected potential DNA fragments binding to TRIM22 by ChIP-Seq technology, and verified the sequencing results by ChIP-qPCR and CUT&Tag technology. In addition, we constructed different TRIM22 mutants to detect the binding of proteins in MAPK signaling pathway. Finally, the therapeutic effect was verified in NOD/SCID mice. The difference between the two groups of data conforming to the normal distribution was tested by Student t-test.
Results

Here, we found for the first time that TRIM22 acts as a transcription factor in the nucleus and binds to exon 2 of the transcript (NM_001204160) of SPHK2 gene to regulate its expression by ChIP-Seq technology, thus indirectly affecting the downstream MAPK signaling pathway. Knockout of TRIM22 using Cas9-sgRNAs resulted in decreased mRNA level of SPHK2 in GBM cells, while overexpression of TRIM22 enhanced it. The ERK1/2 driven luciferase reporter construct identified TRIM22 as a potential activator of MAPK signaling. Knockout and overexpression of TRIM22 regulate the inhibition and activation of MAPK signaling through its RING-finger domain. Co-immunoprecipitation demonstrated that TRIM22 bound to the negative regulator Raf-1 of MAPK signaling and accelerated its degradation by inducing K48-linked ubiquitination. The combination of the two is related to the CC domain and SPRY domain of TRIM22 and the C1D domain of Raf-1. TRIM22 also forms a complex with the downstream regulator ERK1/2 of MAPK and promotes K63-linked ubiquitination, resulting in the phosphorylation of ERK1/2. In addition, in vitro and in situ xenotransplantation models, SPHK2 inhibitor (K145), ERK1/2 inhibitor (Selumetinib) and non-phosphorylated mutant Raf-1S338A inhibited the growth promoting properties of TRIM22 in GBM cell line.
Conclusions

In conclusion, our study shows that TRIM22 regulates SPHK2 transcription as a transcription factor, indirectly affects MAPK signaling, and activates MAPK signaling through post-translational modification of two critical regulators of MAPK signaling in GBM cells.
Evaluation of Neuroprotective And Immunomodulatory Properties of Mesenchymal Stem Cells in An Ex-vivo Retinal Explant Model
Authors: Reboussin Élodie , Buffault Juliette , Brignole-Baudouin Françoise , Goazigo Annabelle Réaux Le , Olmiere Céline , Sahel José-Alain , Parsadaniantz Stéphane Mélik , Baudouin Christophe 
Published: November 2021

Abstract
Background: Mesenchymal stem cells (MSCs) have raised considerable hope for the treatment of glaucoma. Their neuroprotective and regenerative potentials are particularly interesting for this degenerative neuropathy in which retinal ganglion cell (RGC) death leads to a progressive loss of visual field and eventually vision. Yet, despite promising results in animal models, no definitive treatment has been developed, and safety concerns have been reported in human trials. Microglial immunomodulation represents a promising therapeutic approach in which MSCs might play a crucial role. In the present study, we investigated the neuroprotective and immunomodulatory properties as well as the safety of MSCs in an ex vivo neuroretina explant model.Methods: Labeled rat bone marrow MSCs were placed in co-culture with rat retinal explants after optic nerve axotomy. We analyzed the neuroprotective effect of MSCs on RGC survival by immunofluorescence using RBPMS, Brn3a and NeuN markers. Gliosis and retinal microglial activation were measured using GFAP, CD68 and ITGAM mRNA quantification and GFAP, CD68 and Iba1 immunofluorescence staining. We also analyzed the mRNA expression of both ‘M1’ or classically activated state inflammatory cytokines (TNFα, IL1β and IL6), and ‘M2’ or alternatively activated state microglial markers (Arginase 1, IL10, CD163, and TNFAIP6).Results: The number of RGCs was significantly higher in retinal explants cocultured with MSCs compared to the control group at Day 7 following optic nerve axotomy. Retinal explants co-cultured with MSCs showed decreased mRNA markers of gliosis and microglial activation, and immunostaining revealed that GFAP, Iba1 and CD68 were limited to the internal layers of the retina compared to controls showing expression of activated microglia throughout the retina. In addition, MSCs inhibited the M1 phenotype of the microglia. However, edema of the explants was observed in the MSC co-culture group, with an increase of fibronectin labelling at the surface of the explant corresponding to an epiretinal membrane-like phenotype. Conclusion: Using an ex vivo model, we demonstrated a neuroprotective and immunomodulatory effect of MSCs on RGCs. Unfortunately, the presence of MSCs also led to explant edema and epiretinal membrane formation, as described in human trials. Using the MSC secretome might offer the beneficial effects of MSCs without their potential adverse effects, through paracrine signaling.
Social and Housing Determinants of Dengue and Chikungunya in Indian Adults Aged 45 and Above: Analysis of a Nationally Representative Survey (2017-18)
Authors: Paulson Winnie , Balasubramani Karuppusamy , Dixit Rashi , Chellappan Savitha , Behera Sujit Kumar , Nina Praveen Balabaskaran 
Published: November 2021

Abstract
BackgroundDengue and chikungunya (CHIKV) are the two major vector-borne diseases of serious public health concern in India. Studies on socio-economic and housing determinants of dengue and CHIKV at a pan-India level are lacking. Here, we took advantage of the recently carried out Longitudinal Ageing Study in India (LASI) carried across all the States and Union Territories of India to study the social determinants of dengue and CHIKV in India.MethodsLASI-1 (2017-2018) data on the self-reported period prevalence of dengue and CHIKV from 70,865 respondents aged ≥45 years were used for this analysis. The State-wise distribution of dengue and CHIKV was mapped. Prevalence was estimated for each study variable, and the difference was compared using the χ2 test. The adjusted odds ratios (AOR) of the socio-economic and housing variables for dengue and CHIKV were calculated using a multiple logistic regression model.ResultsUrban residence is the major socio-economic determinant of dengue and CHIKV (dengue: AOR: 1.57, 95% CI: 1.17-2.10; CHIKV: AOR 1.56, 95% CI: 1.20-2.02). Wealth status (richest) and less than primary schooling are associated with dengue and CHIKV prevalence. In addition to these factors, social group (scheduled and forward castes) is also associated with CHIKV prevalence. Water-source outside the dwelling (AOR: 1.20, 95% CI: 0.96-1.50), pucca or semi- pucca house type are also associated with increased odds of CHIKV. ConclusionsDespite the limitation that the data is only from adults ≥ 45, this analysis provides important insights into the socio-economic and housing variables that increase the odds of dengue and CHIKV in India. Understanding these determinants may assist in the national planning of prevention and control strategies for dengue and CHIKV.
Three-dimensional Acetabular Bone Defect Classification System Aided with Rapid Prototyping Model and Reliability and Validity Test
Authors: Zhang Jingwei , Hu Yi , Ying Hua , Mao Yuanqing , Zhu Zhenan , Li Huiwu 
Published: November 2021

Abstract
Background: Accurately assessing acetabular defects and designing precise and feasible surgical plans are important before hip revision arthroplasty. With the development of three-dimensional printing, rapid prototyping is a novel technique used to print isometric physical object models. We aimed to propose a three-dimensional acetabular bone defect classification system aided with rapid prototyping and evaluated its reliability and validity.Methods: We reviewed 104 consecutive patients who underwent hip revision arthroplasty in our department between January 2014 and December 2019. Forty five of them had rapid prototyping and were included for reliability and validity test. Three doctors retrospectively evaluated bone defects of these 45 patients with this classification and made surgical plans, and repeated it after 2 weeks. The intra- and inter-observer reliability and the validity to surgical records were assessed using intraclass correlation coefficient or Kappa correlation coefficient.Results: The reliability and validity for classification results were high. The mean initial intraclass correlation coefficient for inter-observer reliability was 0.947, which increased to 0.972 when texted second time. As for inter-observer reliability, it ranged from 0.958 to 0.980. The validity showed high Kappa correlation coefficient of 0.951 to 0.967. When considering detailed surgical plans, the reliability and validity were also high with intraclass correlation coefficient and Kappa correlation coefficient all over 0.9.Conclusions: This three-dimensional acetabulum defect classification was of high reliability and convincing validity. With this classification and objective rapid prototyping models, accurate bone defect assessment and reliable surgical plans were achieved. This classification aided with rapid prototyping could serve as a promising tool for surgeons for preoperative evaluation.
The Gas and Aerosol Phase Composition of Smoke Plumes From The 2019-2020 Black Summer Bushfires and Potential Implications For Human Health
Authors: Simmons Jack B. , Paton-Walsh Clare , Mouat Asher P. , Kaiser Jennifer , Humphries Ruhi S. , Keywood Melita , Sutresna Adithya , Griffith David W.T. , Naylor Travis , Ramirez-Gamboa Jhonathan 
Published: November 2021

Abstract
The 2019-2020 Australian bushfire season was historically large, long, and intense. Smoke from fires burning in southeast Australia blanketed population centres for weeks to months. This study reports the chemical composition in the gas and aerosol phase of aged plumes measured near Wollongong, NSW in early 2020. Enhancement ratios to CO are presented for thirteen species. Plume composition is largely similar to that measured in fresh smoke during previous studies. It is hoped enhancement ratios reported here will assist in plume modelling of landscape scale fires and allow concentration estimates of infrequently measured atmospheric pollutants at monitoring stations. The relative toxicological contribution of species present in the plumes was determined for dilute plume exposure at the measurement site and for concentrated plumes at a heavily impacted population centre case study location. Similar results were determined for both sites. Respirable particles, formaldehyde and acrolein were found to contribute significantly to the toxicological loading, with respirable particles contributing approximately half of the loading. This is a reminder to consider not only the toxicological contributions of particles when studying health impacts of bushfire smoke exposure.
Simple Model to Predict Gestational Diabetes in Nulliparous Women in Early Pregnancy
Authors: ALBA Juan Jesus FERNANDEZ , LARA Maria CASTILLO , HERAS Jose Manuel JIMENEZ , CORTES Rocio MORENO , MACIAS CARMEN GONZALEZ , SANCHEZ Angel VILAR , LAUREANO Florentino CARRAL SAN , CORRAL Luis MORENO 
Published: November 2021

Abstract
BackgroundThe aim of this study was to develop a simple tool using anthropometric, clinical, and analytical variables to predict the risk of gestational diabetes mellitus in the first trimester of pregnancy.MethodsA historical cohort study was conducted with 1,946 Caucasian nulliparous pregnant women at the Department of Obstetrics and Gynecology of the University Hospital of Puerto Real (Cádiz/Spain). The predictive model used was a multivariate logistic regression evaluated by 10-fold cross-validation with five iterations. Receiver-operating characteristic and prediction recall curves were plotted with the predictions of the model. Optimal cut-off points of the receiver-operating characteristic curve were estimated using the Youden Index and minimum distance to point 1,1.ResultsThe final logistic regression model included both maternal weight and height, thyroid-stimulating hormone, family history of diabetes mellitus or hypertension, and the presence of chronic hypertension as predictive variables. The model showed an accuracy of 0.93 and a receiver-operating characteristic area under the curve of 0.791.ConclusionsThis report provides a tool to predict the onset of gestational diabetes mellitus in nulliparous women during the first trimester of pregnancy. This tool is more accurate than the currently available tools and can facilitate the implementation of preventative interventions.
Modified Recipient Blood Flow-Preserved Cross-Leg Anterolateral Thigh Flap Procedure for Complex Lower Extremity Reconstruction
Authors: Zhou Hong-Xiang , He Liang , Yin Dong , Niu Yang , Jin Zhe , Li Jun-Jie , Wang Qian-Kun , Zhou Tao 
Published: November 2021

Abstract
Background

Complex lower limb reconstruction following severe trauma remains a challenge for reconstructive surgeons. Here, we introduce a modified recipient blood flow-preserved cross-leg anterolateral thigh flap procedure and evaluate its clinical efficacy.
Methods

Between January 2013 and December 2019, 22 patients (range 10 to 64 years old) with unilateral lower limb injuries underwent modified recipient blood flow-preserved cross-leg anterolateral thigh flap procedures. Among them, 16 cases were traffic accidents, 5 cases were persistent ulcers, and 1 case was a degloving injury. The arterial pedicle of the flap was prepared in a Y-shaped fashion and microanastomosed to the contralateral posterior tibial artery in a flow-through style. A split-thickness skin graft was applied to wrap the vascular pedicle after anastomosis. The flap was designed in a single or bilobed fashion according to the shape of the defects. The tissue defects ranged from 12 × 6 to 21 × 18 cm2. The vascular pedicle was divided 4 weeks after vascular anastomosis. Doppler ultrasound was performed to evaluate the blood flow of the recipient posterior tibial artery during postoperative follow-up.
Results

All 22 flaps survived. The flap sizes ranged from 14 × 7.5 to 24 × 21 cm2. Eighteen flaps were designed in a single fashion, and four flaps were bilobed. Twenty patients underwent fasciocutaneous flap transplantation, and two underwent musculocutaneous flap transplantation. Two cases developed local lysis of the flap, and the wound healed after further debridement. Direct suture of the donor-site incision was performed in 16 cases, while additional full-thickness skin grafting was performed in the remaining 6 cases. Further bone transport procedures were performed in 15 patients with severe tibia bone defects. Blood flow of recipient posterior tibial arteries was confirmed during follow-up. All flaps recovered sensation at the final follow-up. The postoperative follow-up ranged from 18 to 84 months, and no long-term complications were observed.
Conclusions

The modified recipient blood flow-preserved cross-leg anterolateral thigh flap procedure is an ideal method to repair large tissue defects without sacrificing the major artery of the uninjured lower limb.
Charge Asymmetry of Muons Generated by Laser- Induced Nuclear Processes in Ultra-dense Hydrogen D(0) and p(0)
Authors: Holmlid Leif 
Published: November 2021

Abstract
Laser-induced nuclear reactions in ultra-dense hydrogen H(0) (review in Physica Scripta 2019) give mesons (kaons and pions) which decay to muons. The process which gives the mesons is baryon annihilation (Holmlid, J. Hydrogen Energy 2021; Holmlid and Olafsson, High Energy Density Phys. 2021). The sign of the muons detected depends on the initial baryons, with D(0) in the meson source producing mainly positive muons and p(0) producing mainly negative muons. This charge asymmetry was reported in Holmlid and Olafsson (Heliyon 2019), and has been confirmed by later experiments with a coil current transformer as beam detector , also in another lab (unpublished). The current coil detector would give no signal from the muons if charge symmetry existed. The charge asymmetry of the muons seems first to be at variance with charge conservation. An analysis of the results which includes charge conservation is given here. It agrees with the standard model of particle physics. Using D(0), the asymmetry is, as previously, proposed to be due to capture of µ- in D atoms and D2 molecules. This gives emission of mainly µ+ and a fraction of > 50% of µ+ from D(0). In p(0), the capture rate of µ- is lower than in D(0). The emitted number of µ+ will be decreased by reaction between µ+ and abundant electrons, forming muonium particles. This effect decreases the fraction of emitted µ+ for both p(0) and D(0), and it is proposed to be the main reason for a larger fraction of emitted µ- in the case of p(0).
Effects of Grazing Intensity on Plant-Soil C: N: P Stoichiometry with Precipitation Changes During the Growing Season in Desert Grassland
Authors: Yan Wei , Shi Fengling , Wan Tao 
Published: November 2021

Abstract
Background and aimsHigh-intensity grazing in the Mongolian grassland has led to the general deterioration of biodiversity and ecosystem functioning. Although abundant evidence shows that grazing affects the structure and function of grassland ecosystems, research on the impact of precipitation, especially under drought and overgrazing. MethodsWe examined the effects of heavy grazing, moderate grazing and no grazing on plant communities; plant and soil C, N and P contents; and plant and soil C:N:P stoichiometry in the desert grassland in different years with different amounts of precipitation. ResultsThere was no significant difference in the species diversity between the grazing and no grazing treatment, while the no grazing treatment was significantly higher than the heavy grazing treatment. Compared with the amounts in the no grazing and moderate grazing treatments, the N and P contents of the plants in the heavy grazing treatment were the highest, and the N content of the soil increased. There was a positive correlation between precipitation and the N and P contents of plants and the C and N contents of the soil at 0-10 cm and 10-20 cm. ConclusionsOur study suggest that a large amount of precipitation of plant growth will drive changes in the community species diversity. Grazing promoted the flow of N between plants and the soil, especially under heavy grazing. Under grazing stress, plants maintain the potential of compensatory growth, and precipitation in the peak season of plant growth induces rapid growth, suggesting that precipitation is an important factor driving grazing ecosystems.
Cancer Caregiving and Health Information Technology: An Analysis of the Health Information National Trends (HINTS) Survey
Authors: Nelson Victoria R. , Mitchell Katharine M. , Holtz Bree E 
Published: November 2021

Abstract
Purpose: In this paper, we explore how health technology use impacts informal caregivers’ health and how sociodemographic factors are related, using the Health Information National Trends Survey (HINTS). Methods: Data for this study were obtained from the National Cancer Institutes’ Health Information and National Trends Survey (HINTS 5, Cycle 2, 2018). Participants for the current study were chosen based on their response to one question related to their caregiving status. The sample size was 483 respondents. Variables of interest included caregiver relationship type, general technology use, portal use, and overall health status. Results: The results indicate that there was not a significant difference of caregiving role on portal usage, [F(5,99) = .975, p = .44, η2 = .049], and technology use [F(7, 462)=2.625, p=.01]. This demonstrates that those caregiving for a child are more likely to use technology for health related issues. There was not a significant effect of portal use on caregiver health. However, there was a significant effect of technology use on overall health (t = 2.074, p=.04). There was also a significant effect of demographics on general technology use [F(7, 434)= 14.858, p < .001]. Demonstrating as education and income increases, technology use also increases, and as age increases technology use decreases. Conclusion: This study affirmed that demographic inequalities can negatively impact technology and portal use, which could reduce the burden on caregivers. Therefore, it is important to work to engage cancer patients and their caregivers with technological support and resources.
Value of Radiomics Features From Adrenal Gland and Periadrenal Fat in CT Images for Predicting COVID-19 Prognosis
Authors: zhang Mudan , Yin Xuntao , Li Wuchao , Zha Yan , Zeng Xianchun , Zhang Xiaoyong , Cui Jingjing , Xue Zhong , Wang Rongpin , Liu Chen 
Published: November 2021

Abstract
Background: Endocrine system plays an important role in infectious disease prognosis. Our goal is to assess the value of radiomics features extracted from adrenal gland and periadrenal fat CT images in predicting disease prognosis in patients with COVID-19. Methods: A total of 1,325 patients (765 moderate and 560 severe patients) from three centers were enrolled in the retrospective study. We proposed a 3D cascade V-Net to automatically segment adrenal glands in onset CT images. Periadrenal fat areas were obtained using inflation operations. Then, the radiomics features were automatically extracted. Five models were established to predict the disease prognosis in patients with COVID-19: a clinical model (CM), three radiomics models (adrenal gland model [AM], periadrenal fat model [PM], fusion of adrenal gland and periadrenal fat model [FM]), and a radiomics nomogram model (RN).Data from one center (1,183 patients) were utilized as training and validation sets. The remaining two (36 and 106 patients) were used as 2 independent test sets to evaluate the models’ performance. Results: The auto-segmentation framework achieved an average dice of 0.79 in the test set. CM, AM, PM, FM, and RN obtained AUCs of 0.716, 0.755, 0.796, 0.828, and 0.825, respectively in the training set, and the mean AUCs of 0.754, 0.709, 0.672, 0.706 and 0.778 for 2 independent test sets. Decision curve analysis showed that if the threshold probability was more than 0.3, 0.5, and 0.1 in the validation set, the independent-test set 1 and the independent-test set 2 could gain more net benefits using RN than FM and CM, respectively. Conclusion: Radiomics features extracted from CT images of adrenal glands and periadrenal fat are related to disease prognosis in patients with COVID-19 and have great potential for predicting its severity.