bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effects of Chinese herbal medicine Pugongying for reducing the application of antibiotics in breastfeeding women with acute mastitis: study protocol of a randomized, active-controlled, outcome assessor-blinded trial
Authors: Jin Xinyan , Lu Chunli , Liu Jianping , Fan Yingyi , Xiao Jinhe , Gao Chao , Pei Xiaohua 
Published: August 2021

Abstract
• Background: Acute mastitis influences the health condition and quality of life of the infants and mothers during the lactation. Pugongying (a kind of Chinese patent medicine, Herba Taraxaci) has shown benefits in lactating women with acute mastitis in clinical practice. However, there is no solid evidence to support its effectiveness and safety.• Methods: A three-arm, multicenter, randomized, active-controlled, outcome assessor-blinded clinical trial will be undergoing in three hospitals in Beijing. 306 participants will be randomly assigned into three groups in 1:1:1 ratio with Pugongying alone, cefdinir alone, and combination of Pugongying and cefdinir for 3-day intervention drugs administration. And in combination of Pugongying and cefdinir group, the participants will be administrated with 2-day cefdinir and 3-day Pugongying. The primary outcomes are resolution of fever, visual analogue scale (VAS) scores of breast pain, and the size of the breast mass by palpation. The secondary outcomes are the patency of milk, Traditional Chinese Medicine (TCM) symptoms scores, white blood cell count, the percentage of neutrophil and C-reactive protein, relapse at 3th day of follow up after completion of treatment, and safety assessment including routine blood, liver and renal function and electrocardiography. Besides, the incidence of surgery and the quantity of additional intervention drugs will also be evaluated.• Discussion: The results of this trial are expected to confirm whether Chinese herbal medicine Pugongying could alleviate the symptoms and signs in lactating women with acute mastitis, and they could reduce application of cefdinir in clinical practice.• Trial registration: ClinicalTrials.gov [Home - ClinicalTrials.gov], ID: NCT03756324. Registered on December 18th 2018. https://www.clinicaltrials.gov/ct2/show/NCT03756324?cond=Acute+mastitis&draw=2&rank=1
Factors Affecting the Quality of Life of Gastric Cancer Survivors
Authors: Choi Jahyun , Kim Sanghee , Choi Mona , Hyung Woo Jin 
Published: August 2021

Abstract
Background: The number of gastric cancer survivors has been increasing, and such survivors experience various changes in their lives post-recovery. Adapting to these changes requires appropriate interventions that can improve their quality of life (QoL). This study was conducted to investigate the factors affecting the QoL of gastric cancer survivors to provide basic data for effective intervention.Methods: Data were collected between September 8 and September 29, 2017 from the Gastric Cancer Center at a tertiary hospital. Questionnaire surveys were conducted using the EORTC QLQ-C30/STO22, Self-Efficacy-Scale, Multidimensional Scale of Perceived Social Support, and Quality of Life-Cancer Survivors Questionnaire on gastric cancer survivors who were followed up for three years after gastrectomy. Data were analyzed using descriptive statistics, t-test, ANOVA, Pearson’s correlation coefficient, and stepwise multiple regression analysis.Results: A total of 136 gastric cancer survivors completed the questionnaire survey. There were significant positive correlations of QoL with self-efficacy, functional status, and social support (r=.35, p<.001; r=.53, p<.001; r=.26, p<.001, respectively). There were significant negative correlations of QoL with general symptoms, gastric cancer-specific symptoms (r=-.39, p<.001; r=-.51, p<.001). The regression model explained 48.3% of the QoL, and the affecting factors were gastric cancer-specific symptoms (β=-.397, p<.001), religious belief (β=.299, p<.001), functional status (β=.251, p=.003), and self-efficacy (β=.191, p=.004).Conclusion: This study confirmed that gastric cancer-specific symptoms, spiritual well-being, self-efficacy and functional status affect the QoL of gastric cancer survivors. Hence, these factors should be considered in the interventions to improve the QoL of gastric cancer survivors.
Effect the Radius of Air Holes in Photonic Crystal Fiber on Soliton
Propagation with Different Orders
Authors: Jasim mohammed Salim 
Published: August 2021

Abstract
Photonic crystal fibers (PCFs) with periodic structure, are a never-ending and constantly evolving. in this study was designed fiber photonic crystal is proposed and proven through the Matlab program, which employs the Split-Step Fourier method (SSFM). Among the consequences demonstrated and studied are the solitons in different order, The impact of changing the radius of air holes on the geography of solitone propagation during fiber has studied, and get supercontinuum generation by increasing the value of radius affecting the third-order soliton. This spectral expansion has important in many modern applications, including medical, industrial and military, as well as have an important role in communication systems.
Functional analysis of ectodysplasin-A mutations and involvement of X-chromosome inactivation
Authors: Pan Yuhua , Lu Ting , Peng Ling , Zeng Qi , Huang Xiangyu , Wu Buling , Xiong Fu 
Published: August 2021

Abstract
Objectives: The aim of this study was to identify genetic clues for the causes of familial non-syndromic oligodontia and explore the underlying mechanisms involved, while focusing on the role of human dental pulp stem cells (hDPSCs).Materials and Methods: Candidate gene sequences were obtained by PCR amplification and Sanger sequencing. Functional analysis was conducted, and the pathogenesis associated with EDA mutations in hDPSCs was investigated to explore the impact of the identified mutation on the phenotype. Capillary electrophoresis (CE) was used to detect X chromosome inactivation (XCI) in the blood of female carriers.Results: In this study, we identified an EDA mutation in a Chinese family：the missense mutation c.1013C>T (Thr338Met). Transfection of hDPSCs with a mutant EDA lentivirus decreased the expression of EDA and dentin sialophosphoprotein (DSPP) compared with transfection of control EDA lentivirus. Mechanistically, mutant EDA inhibited the activation of the NF-κB pathway. The CE results showed that symptomatic female carriers had a skewed XCI with a preferential inactivation of the X chromosome that carried the normal allele.Conclusions: In summary, we demonstrated that EDA mutations result in non-syndromic tooth agenesis in heterozygous females and that, mechanistically, EDA regulates odontogenesis through the NF-κB signalling pathway in hDPSCs.Clinical Relevance: Due to the large heterogeneity of tooth agenesis, this study provided a genetic basis for individuals who exhibit similar clinical phenotypes.
Clinical Values of Resting Electrocardiography in Patients with Known or Suspected Chronic Coronary Artery Disease: A Stress Perfusion Cardiac MRI Study
Authors: Kaolawanich Yodying , Thongsongsang Rawiwan , Songsangjinda Thammarak , Boonyasirinant Thananya 
Published: August 2021

Abstract
Background Electrocardiography (ECG) is an essential investigation in patients with chronic coronary artery disease (CAD). However, evidence regarding the diagnostic and prognostic value of ECG in this population is limited. Therefore, we sought to determine whether baseline ECG abnormalities were associated with myocardial ischemia and cardiac events in patients with known or suspected chronic CAD. Methods Consecutive patients with known (n=146) or suspected chronic CAD (n=349) referred for adenosine stress cardiac magnetic resonance (CMR) between 2011 and 2014 were enrolled. Resting ECGs were classified as major, minor and no abnormalities. Predictors of myocardial ischemia on CMR and major adverse cardiac events (MACE) including cardiac death, nonfatal myocardial infarction, hospitalization for heart failure and late revascularization (>180 days after CMR) were evaluated.Results Average age was 69±11 years (51% men). One hundred and eighty-five patients (37.4%) had major and 154 (31.1%) had minor ECG abnormalities. In patients with suspected CAD, myocardial ischemia was present in 83 patients (23.8%). Multivariable analysis demonstrated major ECG abnormality as the strongest predictor of myocardial ischemia (HR 2.88; 95% CI 1.69-4.92; p<0.001). Adding ECG to clinical pretest probability models improved the prediction of myocardial ischemia in ROC analyses (p=0.04). In the whole cohort (n=495), 91 MACE occurred during the median follow-up period of 4.8 years. Multivariable analysis showed that diastolic blood pressure, history of heart failure, prior revascularization, ischemia, myocardial scarring, and major ECG abnormality were independent predictors of MACE.Conclusion Abnormal resting ECG is common in patients with known or suspected chronic CAD. ECG had important diagnostic and prognostic values in this population.
Long Noncoding RNA PTTG3P Promotes the Development of Childhood Asthma by Targeting the miR-192-3p/CCNB1 Axis
Authors: Dai Bing , Sun Feifei , Cai Xuxu , Li Chunlu , Liu Fen , Shang Yunxiao 
Published: August 2021

Abstract
Background: Increasing studies have suggested that long non-coding RNAs (lncRNAs) affect the regulation of immune responses, airway inflammation, and other pathological processes of asthma. In this study, we investigated the functions of the lncRNA PTTG3P in the progression of childhood asthma.Methods: A competitive endogenous RNA network PTTG3P/miR-192-3p/CCNB1 was identified via bioinformatics analyses. Real-time qPCR and western blot were used to quantify gene and protein expression levels, respectively. Cell counting kit‑8 and transwell assays were used to evaluate the proliferation and migration abilities of bronchial epithelial cells (16HBE). Double luciferase reporter gene assay was used to validate the predictive targets in PTTG3P, miR-192-3p, and CCNB1.Results: PTTG3P was highly expressed in the peripheral blood of children with asthma. Knocking down PTTG3P could inhibit the epithelial-mesenchymal transition (EMT), proliferation, and migration of 16HBE cells. Mechanistically, PTTG3P promoted childhood asthma progression by targeting the miR-192-3p/CCNB1 axis.Conclusions: Childhood asthma development can be stemmed by targeting the PTTG3P/miR-192-3p/CCNB1 axis. This study provides potential diagnosis and treatment biomarkers for childhood asthma.
Article A Genome-Wide Association Study To Identify Candidate Genes for Metabolic Disorders in Offspring by In Vitro Fertilization
Authors: Liu Lihong , Ha Siyao , Li Zhiling , Li MingQing 
Published: August 2021

Abstract
Background

In vitro fertilization (IVF) processes increase offspring's short-term and long-term health risks, but their mechanisms remain unclear.
Methods

We conducted a bibliometric analysis to determine the landscape of IVF offspring health. Subsequently, a bioinformatics method was utilized to identify the co-genes properties and biological function mechanisms of IVF and type 2 diabetes mellitus (T2DM). Finally, we predicted compounds against key targets and performed multiple validations of the mechanisms underlying IVF offspring health risks.
Results

We identified 15 genes associated with T2DM, and their biological functions are primarily associated with lipid metabolism. We also identified the properties of co-genes, modified characteristics, confirmed a conserved motif, identified 3 SNPs sites, and determined the three core genes, APOA1, APOB, and APOE, which were mainly correlated with metabolic and cardiovascular diseases. In addition, we predicted drugs that may improve metabolic abnormalities in IVF offspring.
Conclusion

The impact of aberrant lipid metabolism in offspring after IVF therapy warrants additional investigation, particularly in terms of long-term health consequences and possible mechanisms.
Psychosocial Risk, Symptom Burden, and Concerns in Families Affected by Childhood Cancer
Authors: Russell K. Brooke , Patton Michaela , Tromburg Courtney , Zwicker Hailey , Guilcher Gregory M. T. , Bultz Barry D. , Schulte Fiona 
Published: August 2021

Abstract
PURPOSE: The revised Psychosocial Assessment Tool (PATrev) is a common family-level risk-based screening tool for pediatric oncology that has gained support for its ability to predict, at diagnosis, the degree of psychosocial support a family may require throughout the treatment trajectory. However, ongoing screening for symptoms and concerns (e.g., feeling alone, understanding treatment) remain underutilized. Resource limitations necessitate triaging and intervention based on need and risk. Given the widespread use of the PATrev, we sought to explore the association between family psychosocial risk, symptom burden (as measured by the revised Edmonton Symptom Assessment System; ESAS-r), and concerns (as measured by the Canadian Problem Checklist; CPC). METHODS: Families (n = 85) with children between 2–18 years of age (M = 11.98, male: 62.4%) on or off treatment for cancer were recruited from the Alberta Children’s Hospital. One parent from each family completed the PATrev and the CPC. Participants 8–18 years of age completed the ESAS-r. RESULTS: Risk category (unviersal/low risk = 67.1%, targeted/intermediate risk = 21.1%, clinical/high risk = 5.9%), predicted symptom burden (F[2, 63.07] = 4.57, p = .014) and concerns (F[2, 80.08] = 16.34, p < .001), such that universal risk was associated with significantly lower symptom burden and fewer concerns. CONCLUSION: Family psychosocial risk is associated with cross-sectionally identified concerns and symptom burden, suggesting that resources might be prioritized for families with the greatest predicted need. Future research should evaluate the predictive validity of the PATrev to identify longitudinal concerns and symptom burden throughout the cancer trajectory.
Identifying and Responding To Anxiety and Depression in Adult Cancer Patients: Development, Acceptability and Uptake of An On-Line Communication Skills Education Program Targeting Challenging Conversations for Oncology Health Professionals
Authors: Shaw Joanne , Allison Karen , Cuddy Jessica , Lindsay Toni , Grimison Peter , Shepherd Heather , Butow Phyllis , Shaw Tim , Baychek Kate , Group ADAPT Program , Kelly Brian 
Published: August 2021

Abstract
Background: Anxiety and depression screening and management in cancer settings occurs inconsistently in Australia. We developed a clinical pathway (ADAPT CP) to promote standardized assessment and response to affected patients and enhance uptake of psychosocial interventions. Health professional education is a common strategy utilised to support implementation of practice change interventions. Health professional education is a common strategy utilised to support implementation of practice change interventions. We developed an interactive on-line education program to support staff communication and confidence with anxiety/depression screening and referral prior to the ADAPT CP being implemented in 12 oncology services participating in the ADAPT CP cluster randomised controlled trial (CRCT). The aim of this research was to assess acceptability and uptake of the education program.Patient Involvement: Although the wider ADAPT Program included patient consumers on the Steering Committee, in the context of this research consumer engagement included health professionals working in oncology. These consumers contributed to resource development.Methods: Development was informed by oncology and communication literature. The five online modules were pilot tested with 12 oncology nurses who participated in standardised medical simulations. Acceptability and uptake were assessed across the 12 Oncology services participating in the ADAPT CRCT. Results: During pilot testing the online training was reported to be acceptable and overall communication and confidence improved for all participants post training. However, during the ADAPT CRCT uptake was low (7%; n=20). Although those who accessed the training reported it to be valuable, competing demands and online format reportedly limited HPs’ capacity and willingness to undertake training.Conclusions: This interactive on-line training provides strategies and communication skills for front-line staff to guide these important conversations. Building workforce skills, knowledge and confidence is crucial for the successful implementation of practice change interventions. However, despite being acceptable during pilot testing, low uptake in real world settings highlights organisational support and incentivisation for frontline staff to undertake training is critical for wider engagement. A multimodal approach to delivery of training to cater for staff preferences for face to face and/or online staff may maximise training uptake and increase effectiveness of training interventionsTrial Registration: Pilot study ACTRN12616001490460 (27/10/2016). ADAPT RCT ACTRN12617000411347(22/03/2017)
Calibrating a Discrete-Event Simulation for Quantification of Sex-Specific Colorectal Neoplasia Development
Authors: Vivas-Valencia Carolina , Kong Nan , Sai Aditya , Imperiale Thomas F 
Published: August 2021

Abstract
Background: Medical evidence collected from new observational studies can sometimes significantly alter our understanding of disease incidence and progression. This requires efficient and accurate calibration of disease models to help quantify the differences between observed cohorts. However, in model calibration, it is common to encounter overfitting with many model parameters but few observational outcomes. Additionally, the difficulty in evaluating fitting performance is significant due to a large degree of outcome variation and expensive computations for even a single simulation run. Methods: We developed a two-phase calibration procedure to address the above challenges. As a proof-of-the-concept study, we verified the procedure with a discrete-event-simulation-based study on sex-specific colorectal neoplasia development. For the study, we estimated eight disease model parameters that govern colorectal adenoma incidence risk and growth rates at three distinct states: non-advanced, advanced adenoma, and adenoma becoming cancerous. For the calibration, we defined the likelihood measure by a relative weighted sum-of-squares difference between the three actual prevalence values reported in a recent publication and those predicted by a discrete-event colorectal cancer simulation. In phase I of the calibration procedure, we performed a series of low-dimensional sampling-based grid searches to identify reasonably good candidate parameter designs. In phase II, we performed a local search-based approach to further improve the model fit.Results: Overall, our two-phase procedure showed better goodness of fit than a straightforward implementation of the Nelder-Mead algorithm, yielding a 10-fold reduction in calibration error (0.0025 vs. 0.0251 for an all-white mixed-family-history male cohort on the likelihood measure defined above). Further, the two-phase procedure was more effective in calibrating a validated simulation model for a female cohort than a male cohort. Finally, in phase II, performing local search on each of the parameters sequentially is more effective than searching the entire parameter space simultaneously. Conclusions: The proposed two-phase calibration procedure is effective for estimating computationally expensive stochastic dynamic disease models. In addition, initial parameter search range truncation and sensitivity analysis on various parameters can be computationally cost-effective.
Tissue-specific ramp sequences correspond with increased gene expression in humans and SARS-CoV-2
Authors: Miller Justin , Meurs Taylor , Hodgman Matthew , Song Benjamin , Ebbert Mark , Kauwe John , Ridge Perry 
Published: August 2021

Abstract
Translational ramp sequences are essential regulatory elements that have yet to be characterized in specific tissues. Ramp sequences increase gene expression by evenly spacing ribosomes and slowing initial translation. Therefore, the relative codon adaptiveness within different tissues changes the existence of a ramp sequence without altering the underlying genetic code. Here, we present the first comprehensive analysis of tissue and cell type-specific ramp sequences, and report 3,108 genes with ramp sequences that change between tissues and cell types. The Ramp Atlas (https://ramps.byu.edu/) is an accompanying web portal that allows researchers to query ramp sequences in 18,388 genes across 62 tissues and 66 cell types. We also identified seven SARS-CoV-2 genes and seven human SARS-CoV-2 entry factor genes with tissue-specific ramp sequences that may help explain viral proliferation within those tissues. We anticipate that The Ramp Atlas will facilitate future tissue-specific ramp sequence analyses to develop targeted therapeutics for human disease.
Vitamin D Receptor Gene Polymorphism as a Risk Factor for Chronic Periodontitis
Authors: Hamrun Nurlindah , Ruslin Muhammad , Marlina Erni , Oktawati Sri , Saito Takashi , Yusuf Andi Siti Hajrah , Ou Keng-Liang 
Published: August 2021

Abstract
Background: The aim of this study was to investigate vitamin D receptor (VDR) gene polymorphism as a risk factor associated with chronic periodontitis (CP) and to determine the effect of VDR gene polymorphism on phenotypic CP.Methods: This study is a case-control design that included 162 adults divided into two groups: patients with CP (case group) and patients without CP (control group). Venous blood and DNA were obtained from individual samples. The gene polymorphism was determined using Restricted Fragment Length Polymorphism-Polymerase Chain Reaction (RFLP-PCR) and DNA sequencing to identify endonuclease restrictions in exon 9 (TaqI). The data were analyzed using an independent t-test and Fisher’s exact test. The odds ratio (OR) was used to calculate the risk of VDR gene polymorphism in CP. Results: VDR gene polymorphism was detected in patients with CP and a TT genotype (86.4%), Tt genotype (12.4%), and tt genotype (1.2%). The case group with TT and Tt genotypes had an OR of 12.5 (95% CI:1.6–99.8) of having CP compared to the control group (P<0.05).Conclusions: VDR gene polymorphisms (the TT and Tt genotypes) are risk factors associated with individual susceptibility to CP.