bluuebunny/crossref_metadata_2025_split huggingface dataset:
Temperature Dependent On Activated Carbon For Their Biological Activity And Photocatalytical Degradation of Methylene Blue: A Novel Approach To Green Synthesis
Authors: Amalanathan.M  , Aravind.M  , Mary.M Sony Michael , Alshgari Razan A. , Alothman Asma A. , Zahrani Rahaf S. AL , Islam Mohammad Ataul 
Published: August 2021

Abstract
In this work, jasmine flower derived activated carbon were successfully synthesized by hydrothermal carbonization process at the different annealing temperature. The Crystallinity, phase, structural, morphological and optical properties of activated carbon were investigated using X-ray diffraction (XRD), Fourier Transform Infrared spectroscopy (FT-IR), Scanning electron microscopy (SEM), Transmission electron microscope (TEM), and UV-visible spectroscopy analysis. The graphitic phase of carbon was obtained from the XRD pattern. Surface morphology reveals irregular-shaped nanoparticles. The photodegradation of methylene blue (MB) was carried out under the visible light irradiation technique to study its photocatalytic activity. The activated carbon obtained at 400oC, 500oC and 600oC shows a photocatalytic degradation efficiency of 86%, 90%, and 94%, respectively. Antibacterial activity of activated carbon was examined against S. Aureus (MTCC-737) and E-Coli (MTCC- 443) microbial pathogens, and their potent antibacterial activity was examined from the zone of inhibition layer.
Differentiated SH-SY5Y Cells for High-Throughput Screening in a 96-Well Plate Format: Protocol Optimisation and Application for Neurite Outgrowth Assays
Authors: Dravid Anusha , Raos Brad , Svirskis Darren , O’Carroll Simon J 
Published: August 2021

Abstract
Neuronal models are a crucial tool in neuroscientific research, helping to elucidate the molecular and cellular processes involved in disorders of the nervous system. Adapting these models to a high-throughput format enables simultaneous screening of multiple agents within a single assay. SH-SY5Y cells have been widely used as a neuronal model, yet commonly in an undifferentiated state that is not representative of mature neurons. Differentiation of the SH-SY5Y cells is a necessary step to obtain cells that express mature neuronal markers. Despite this understanding, the absence of a standardised protocol has limited the use of differentiated SH-SY5Y cells in high-throughput assay formats. Here, we describe a protocol to differentiate and re-plate SH-SY5Y cells within a 96-well plate for high-throughput screening. SH-SY5Y cells seeded at an initial density of 2,500 cells/well in a 96-well plate provide sufficient space for neurites to extend, without impacting cell viability. Room temperature pre-incubation for 1 hour improved the plating homogeneity within the well and the ability to analyse neurites. We then demonstrated the efficacy of this protocol by optimising it further for neurite outgrowth analysis. The presented protocol achieves homogenously distributed differentiated SH-SY5Y cells, useful for researchers using these cells in high-throughput screening assays.
Low-concentration T-2 Toxin Attenuates Pseudorabies Virus Replication in Porcine Kidney 15 Cells
Authors: Xiong Kuankuan , Tan Lei , Yi Siliang , Wu Yingxin , Hu Yi , Wang Aibing , Yang Lingchen 
Published: August 2021

Abstract
Pseudorabies, caused by pseudorabies virus (PRV), is the main highly infectious disease that severely affects the pig industry globally. T-2 toxin (T2), a significant mycotoxin, is widely spread in food and feeds and shows high toxicity to mammals. The potential mechanism of the interaction between viruses and toxins is of great research value because revealing this mechanism may provide new ideas for their joint prevention and control. In this study, we investigated the effect of T2 on PRV replication and the mechanism of action. The results showed that at a low dose (10 nM) T2 had no significant effect on porcine kidney 15 (PK15) cell viability. However, this T2 concentration alleviated PRV-induced cell injury and increased cell survival time. Additionally, the number of PK15 cells infected with PRV was significantly reduced by T2 treatment. Similarly, T2 significantly decreased the copy number of PRV. Investigation of the mechanism revealed that 10 nM T2 significantly inhibited PRV replication and led to downregulation of oxidative stress- and apoptosis-related genes. These results suggest that oxidative stress and apoptosis are involved in the inhibition of PRV replication in PK15 cells by low-concentration T2. Taken together, we demonstrated the protective effects of T2 against PRV infection. A low T2 concentration inhibited the replication of PRV in PK15 cells, and this process was accompanied by downregulation of by the oxidative stress and apoptosis signaling pathways. Our findings partly explain the interaction mechanism between T2 and PRV, relating to oxidative stress and apoptosis, though further research is required.
Safety And Efficacy of ICIs Plus Anlotinib Vs Anlotinib Alone As Third-Line Treatment In Extensive-Stage Small Cell Lung Cancer: A Retrospective Study
Authors: Chen Qing , Li Yan , Zhang Wenjie , Wang Chen , Yang Shengjie , Guo Qisen 
Published: August 2021

Abstract
Purpose: The objective of this study was to evaluate safety and efficacy of Immune checkpoint inhibitors (ICIs) plus anlotinib as third-line treatment in extensive-stage small cell lung cancer (ES-SCLC).Methods: 120 patients with ES-SCLC admitted to Shandong Cancer Hospital between January 2019 and December 2020, were retrospectively analyzed. They were divided into observation group (n=62) and control group (n=58) according to different treatment plans. Observation group were given ICIs plus anlotinib, while control group were given anlotinib alone. The primary endpoint of the study was progression free survival (PFS), and the secondary endpoints were objective response rate (ORR) and disease control rate (DCR). Efficacy evaluation was carried out every 2 cycles of the treatment. Univariate and multivariate analyses were performed to determine the prognostic factors. The main adverse events were evaluated according to the Common Terminology Criteria for Adverse Events version 5.0.Results: In observation group and control group, the DCR were 87.1% and 72.4% (p = 0.044), and the ORR were 19.4% and 6.9% (p = 0.045), respectively. The median PFS was longer in observation group (7.53 months) than that in control group (4.57 months) (p = 0.0033). In Cox regression analysis, the Eastern Cooperative Oncology Group performance status score, brain metastases and metastatic sites were prognostic factors of ICIs plus anlotinib. Compared with control group, Grade 3-4 adverse events did not increase significantly in observation group. Treatment-related adverse events were tolerable and controllable.Conclusion: ICIs plus anlotinib showed promising efficacy and low toxicity in third-line treatment of ES-SCLC.
Comparison of the developmental potential of the morphological good embryos between normal-responders with low and high rate of MG day 3 embryos formation
Authors: Dai Xiuliang , Xia Xiyang , Gao Tingting , Yu Chunmei , Cao Fang , Li Tianfu , Li Lingjun , Wang Yufeng , Chen Li 
Published: August 2021

Abstract
Background

To determine whether the morphological good (MG) day 3 embryos or blastocysts from normal responders with the low rate of MG day 3 embryos formation (LRTD3) and high rate of MG day 3 embryos formation (HRTD3) have similar developmental potential.
Methods

Oocyte retrieval cycles were ranked by the rate of MG day 3 embryos (MG day 3 embryos/ 2PN zygotes), cycles in the bottom25th percentile was defined as low potential group (L) while cycles in the top 25th percentile was defined as high potential group (H). The basic characteristics of patients and cycles, blastocyst formation rate, and clinical outcomes of transfer of the MG blastocysts or day 3 embryos were compared between the H and L groups.
Results

The overall characteristics of patients and cycles were grossly comparable between the H and L group in patients with MG day 3 embryo transfer or blastocysts transfer. In patients with transfer of MG day 3 embryos, patients with LRTD3 showed decreased rate of blastocyst formation and similar clinical outcomes of direct transfer of MG day 3 embryos, compared to patients with HRTD3. In patients with transfer of MG blastocysts, patients with LRTD3 showed decreased rate of blastocyst formation, as well as decreased rate of implantation and livebirth of transfer of MG blastocysts. compared to patients with HRTD3.
Conclusion

We concluded that the quality of MG day 3 embryos or MG blastocysts from patients with LRTD3 was reduced, compared to patients with HRTD3. However, it seems that direct transfer of MG day 3 embryos effectively avoid the flaws of poor quality of MG day 3 embryos from patients with LRTD3. Therefore, our study support the strategy of transfer of MG day 3 embryos rather than expended culture for patients with LRTD3.
BRCA2 C-terminal RAD51-binding Domain Confers Resistance to DNA-damaging Agents
Authors: Yoshikawa Yasunaga , Morimatsu Masami , Tanaka Arisa , Morioka Ryo , Orino Koichi 
Published: August 2021

Abstract
Breast cancer type 2 susceptibility (BRCA2) protein is crucial for initiating DNA damage repair after chemotherapy with DNA interstrand cross-linking agents or X-ray irradiation. These treatments induce double-stranded breaks in genomic DNA. BRCA2 contains a C-terminal RAD51-binding domain (CTRBD) that interacts with RAD51-oligomer containing nucleofilaments. RAD51 nucleofilaments are essential for the homologous recombination repair of double-stranded DNA damage. In this study, we investigated the effects of expressing the CTRBD in cells exposed to X-ray irradiation and mitomycin C treatment. Surprisingly, expressing the BRCA2 CTRBD in HeLa cells increased their resistance to X-ray irradiation and mitomycin C. To determine the ability of the BRCA2 CTRBD to mediate DNA damage repair, the endogenous BRCA2 was depleted by shRNA. No significant differences were observed between the sensitivities of the BRCA2-depleted cells with or without expressing BRCA2 CTRBD. Thus, the resistance to X-ray irradiation conferred by the exogenous CTRBD required endogenous BRCA2 expression. To the best of our knowledge, our study is the first to report the ability of the BRCA2 functional domain to confer resistance to X-ray irradiation and mitomycin C treatment. This peptide may be useful in the protection of cells against X-ray irradiation or chemotherapeutic agents.
POETIZ@NDO: POESIA NAS MÍDIAS SOCIAIS NA PERSPECTIVA DA LITERACIA FAMILIAR
Authors: De Azevedo Machado Flávia Christiane , Ferreira de Figueiredo Alessandra Aniceto , Ferreira Brenda Nayara Carlos , De Barros Medeiros Ideize , Lima Ribeiro Iramara , Abreu de Carvalho Letícia , Dantas e Silva Lopes Maria Antônia , Dantas Campelo Maria Carolina , Bezerra Teles Mariana , Pinheiro de Paiva Neta Mariana , Ferreira de Oliveira Suelen , De Araújo Lucena Túlio 
Published: August 2021

O projeto de Extensão Edusaúde UFRN desenvolve atividades de educação popular em saúde, utilizando o instagram @edusaudeufrn. Dentre as atividades, há o “poetiz@ndo” atividade de literacia em saúde, desenvolvida no período de Setembro a Outubro de 2020, onde poesias de autores brasileiros, voltadas ao público infantil foram declamadas, gravadas em vídeos e postadas. Além das poesias, divulgaram-se biografias dos poetas. A interação do público foi inferida pelas curtidas, visualizações, comentários. O poetiz@ndo ao veicular 11 poesias, de oito autores e suas biografias, obteve 994 visualizações e 133 curtidas nas declamações e 229 curtidas nas biografias. Assim, atinge o objetivo de desmistificar a poesia como gênero literário restrito as elites socais e contribuir para a formação acadêmica, trabalhando habilidades de comunicar-se, trabalhar em equipe, planificar ações.

Palavras-Chave: Poesia. Educação em Saúde. Mídias sociais.
The Application of Continuous Improvement (CI) Methodology in Small-Scale (SME) Jewellery / Gold Fabricators Refiners toward Efficient Work Process in Waste Management.
Authors: Kaspin Saadiah , Khairi Hanif , Hassan Oskar Hasdinor 
Published: August 2021

This paper presents the rationale of the continuous improvement (CI) system to be applied for the small-scale jewellery industry in the state of Kelantan State of Malaysia. The manufacturer’s inefficiency during the fabrication process directly contributes to the potentially lost gold in scrap (waste) form that would eventually affect the producer's profit margin and may lead to a compromised quality of end products. Hence, the adoption of appropriate quality management applications and good practices are key to enhance business and operational efficiency towards sustainability of gold scrap and waste handling that may lead to potential economic loss.
Keywords: Sustainability; gold waste; continuous improvement (CI); jewellery industry
eISSN: 2398-4287© 2021. The Authors. Published for AMER ABRA cE-Bs by e-International Publishing House, Ltd., UK. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). Peer–review under responsibility of AMER (Association of Malaysian Environment-Behaviour Researchers), ABRA (Association of Behavioural Researchers on Asians/Africans/Arabians) and cE-Bs (Centre for Environment-Behaviour Studies), Faculty of Architecture, Planning & Surveying, Universiti Teknologi MARA, Malaysia.
DOI: https://doi.org/10.21834/ebpj.v6iSI4.2899
Mobile Phone Addiction: Determinant Factors among Students in a Malaysian Public University
Authors: Chan Yuen Fook , Narasuman Suthagar 
Published: August 2021

The present study was conducted to examine mobile addiction determinant factors among university students in higher education in Malaysia. A total of 162 students responded to the online survey questionnaire. The study employed a quantitative-based research method to obtain data, and the research instruments consist of a set of combined questionnaires adopted from two Ph.D. studies. The correlation result between mobile addiction and attitude showed a stronger association than with the purpose of use. However, linear regression only indicated a significant contribution of students’ attitude towards mobile addiction variance.
Keywords: Attitude; Mobile addiction; Purpose of Use: University students
eISSN: 2398-4287© 2021. The Authors. Published for AMER ABRA cE-Bs by e-International Publishing House, Ltd., UK. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). Peer–review under responsibility of AMER (Association of Malaysian Environment-Behaviour Researchers), ABRA (Association of Behavioural Researchers on Asians/Africans/Arabians) and cE-Bs (Centre for Environment-Behaviour Studies), Faculty of Architecture, Planning & Surveying, Universiti Teknologi MARA, Malaysia.
DOI: https://doi.org/10.21834/ebpj.v6iSI4.2905
Quranic Circles Attendance: A tool for stress relief among Malaysian medical students?
Authors: Katiman Diana , Osman Muhamed T , Yunus Raudah , Nawawi Hapizah 
Published: August 2021

Medical students may attend Quranic Circles (QC) to cope with stress. Study objectives were to determine its prevalence and investigate its relationship with students' perception of stress and quality of life (QoL). 339 students responded. 78.8% were female and median age was 22. 118 (34.8%) students reported higher stressor levels. 73% attended QC at least a few times a month. They had high spirituality/religiosity (S/R) scores (mean Duke University Religion Index (DUREL) score = 22.43 + 3.23). They have lower stressor levels, higher S/R scores, and better QoL. Clinical year students recorded a better QoL than the pre-clinical year students.
Keywords: Malaysia, Medical students, Quranic Circles, Stress
eISSN: 2398-4287© 2021. The Authors. Published for AMER ABRA cE-Bs by e-International Publishing House, Ltd., UK. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). Peer–review under responsibility of AMER (Association of Malaysian Environment-Behaviour Researchers), ABRA (Association of Behavioural Researchers on Asians/Africans/Arabians) and cE-Bs (Centre for Environment-Behaviour Studies), Faculty of Architecture, Planning & Surveying, Universiti Teknologi MARA, Malaysia.
DOI: https://doi.org/10.21834/ebpj.v6iSI4.2911
Medical Tourism from the Perspectives of Industry Players: How Critical is Government Support?
Authors: Rosnan Herwina , Yusof Norzayana 
Published: August 2021

The present study aims to derive the role of the government in developing Malaysian medical tourism. Nine semi-structured interviews were conducted, and data were analysed thematically using Atlas.ti version 8. The study derived four main challenges facing private hospitals and healthcare facilitators, which are regulatory burden, scarcity of health professionals, poor collaboration with state government and low cooperation from other agencies. These challenges then drew the government’s role accordingly. Hence, this article strongly calls for regular dialogues between government agencies, private hospitals and healthcare facilitators to ensure that all stakeholders are on the same page about developing the industry.
Keywords: Medical Tourism, Private Hospitals, Healthcare Facilitators, Government
eISSN: 2398-4287© 2021. The Authors. Published for AMER ABRA cE-Bs by e-International Publishing House, Ltd., UK. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). Peer–review under responsibility of AMER (Association of Malaysian Environment-Behaviour Researchers), ABRA (Association of Behavioural Researchers on Asians/Africans/Arabians) and cE-Bs (Centre for Environment-Behaviour Studies), Faculty of Architecture, Planning & Surveying, Universiti Teknologi MARA, Malaysia.
DOI: https://doi.org/10.21834/ebpj.v6iSI4.2912
The Association Between Patients' eHealth Literacy and Satisfaction With Shared Decision-making and Well-being: Multicenter Cross-sectional Study
Authors: Xu Richard Huan , Zhou Ling-Ming , Wong Eliza Lai-Yi , Wang Dong 
Published: August 2021

Background
Although previous studies have shown that a high level of health literacy can improve patients’ ability to engage in health-related shared decision-making (SDM) and improve their quality of life, few studies have investigated the role of eHealth literacy in improving patient satisfaction with SDM (SSDM) and well-being.

Objective
This study aims to assess the relationship between patients’ eHealth literacy and their socioeconomic determinants and to investigate the association between patients’ eHealth literacy and their SSDM and well-being.

Methods
The data used in this study were obtained from a multicenter cross-sectional survey in China. The eHealth Literacy Scale (eHEALS) and Investigating Choice Experiments Capability Measure for Adults were used to measure patients’ eHealth literacy and capability well-being, respectively. The SSDM was assessed by using a self-administered questionnaire. The Kruskal-Wallis one-way analysis of variance and Wilcoxon signed-rank test were used to compare the differences in the eHEALS, SSDM, and Investigating Choice Experiments Capability Measure for Adults scores of patients with varying background characteristics. Ordinary least square regression models were used to assess the relationship among eHealth literacy, SSDM, and well-being adjusted by patients’ background characteristics.

Results
A total of 569 patients completed the questionnaire. Patients who were male, were highly educated, were childless, were fully employed, were without chronic conditions, and indicated no depressive disorder reported a higher mean score on the eHEALS. Younger patients (SSDM≥61 years=88.6 vs SSDM16-30 years=84.2) tended to show higher SSDM. Patients who were rural residents and were well paid were more likely to report good capability well-being. Patients who had a higher SSDM and better capability well-being reported a significantly higher level of eHealth literacy than those who had lower SSDM and poorer capability well-being. The regression models showed a positive relationship between eHealth literacy and both SSDM (β=.22; P<.001) and well-being (β=.26; P<.001) after adjusting for patients’ demographic, socioeconomic status, lifestyle, and health status variables.

Conclusions
This study showed that patients with a high level of eHealth literacy are more likely to experience optimal SDM and improved capability well-being. However, patients’ depressive status may alter the relationship between eHealth literacy and SSDM.
The Extent of User Involvement in the Design of Self-tracking Technology for Bipolar Disorder: Literature Review
Authors: Majid Shazmin , Reeves Stuart , Figueredo Grazziela , Brown Susan , Lang Alexandra , Moore Matthew , Morriss Richard 
Published: August 2021

Background
The number of self-monitoring apps for bipolar disorder (BD) is increasing. The involvement of users in human-computer interaction (HCI) research has a long history and is becoming a core concern for designers working in this space. The application of models of involvement, such as user-centered design, is becoming standardized to optimize the reach, adoption, and sustained use of this type of technology.

Objective
This paper aims to examine the current ways in which users are involved in the design and evaluation of self-monitoring apps for BD by investigating 3 specific questions: are users involved in the design and evaluation of technology? If so, how does this happen? And what are the best practice ingredients regarding the design of mental health technology?

Methods
We reviewed the available literature on self-tracking technology for BD and make an overall assessment of the level of user involvement in design. The findings were reviewed by an expert panel, including an individual with lived experience of BD, to form best practice ingredients for the design of mental health technology. This combines the existing practices of patient and public involvement and HCI to evolve from the generic guidelines of user-centered design and to those that are tailored toward mental health technology.

Results
For the first question, it was found that out of the 11 novel smartphone apps included in this review, 4 (36%) self-monitoring apps were classified as having no mention of user involvement in design, 1 (9%) self-monitoring app was classified as having low user involvement, 4 (36%) self-monitoring apps were classified as having medium user involvement, and 2 (18%) self-monitoring apps were classified as having high user involvement. For the second question, it was found that despite the presence of extant approaches for the involvement of the user in the process of design and evaluation, there is large variability in whether the user is involved, how they are involved, and to what extent there is a reported emphasis on the voice of the user, which is the ultimate aim of such design approaches. For the third question, it is recommended that users are involved in all stages of design with the ultimate goal of empowering and creating empathy for the user.

Conclusions
Users should be involved early in the design process, and this should not just be limited to the design itself, but also to associated research ensuring end-to-end involvement. Communities in health care–based design and HCI design need to work together to increase awareness of the different methods available and to encourage the use and mixing of the methods as well as establish better mechanisms to reach the target user group. Future research using systematic literature search methods should explore this further.