bluuebunny/crossref_metadata_2025_split huggingface dataset:
Association of Medicaid expansion under the Affordable Care Act with access to elective spine surgical care
Authors: Greenberg Jacob K. , Brown Derek S. , Olsen Margaret A. , Ray Wilson Z. 
Published: October 2021

OBJECTIVE
The Affordable Care Act expanded Medicaid eligibility in many states, improving access to some forms of elective healthcare in the United States. Whether this effort increased access to elective spine surgical care is unknown. This study’s objective was to evaluate the impact of Medicaid expansion under the Affordable Care Act on the volume and payer mix of elective spine surgery in the United States.

METHODS
This study evaluated elective spine surgical procedures performed from 2011 to 2016 and included in the all-payer State Inpatient Databases of 10 states that expanded Medicaid access in 2014, as well as 4 states that did not expand Medicaid access. Adult patients aged 18–64 years who underwent elective spine surgery were included. The authors used a quasi-experimental difference-in-difference design to evaluate the impact of Medicaid expansion on hospital procedure volume and payer mix, independent of time-dependent trends. Subgroup analysis was conducted that stratified results according to cervical fusion, thoracolumbar fusion, and noninstrumented surgery.

RESULTS
The authors identified 218,648 surgical procedures performed in 10 Medicaid expansion states and 118,693 procedures performed in 4 nonexpansion states. Medicaid expansion was associated with a 17% (95% CI 2%–35%, p = 0.03) increase in mean hospital spine surgical volume and a 23% (95% CI −0.3% to 52%, p = 0.054) increase in Medicaid volume. Privately insured surgical volumes did not change significantly (incidence rate ratio 1.13, 95% CI −5% to 34%, p = 0.18). The increase in Medicaid volume led to a shift in payer mix, with the proportion of Medicaid patients increasing by 6.0 percentage points (95% CI 4.1–7.0, p < 0.001) and the proportion of private payers decreasing by 6.7 percentage points (95% CI 4.5–8.8, p < 0.001). Although the magnitude of effects varied, these trends were similar across procedure subgroups.

CONCLUSIONS
Medicaid expansion under the Affordable Care Act was associated with an economically and statistically significant increase in spine surgery volume and the proportion of surgical patients with Medicaid insurance, indicating improved access to care.
Evaluating stability of the craniovertebral junction after unilateral C1 lateral mass resection: implications for the direct lateral approach
Authors: Soni Pranay , Loss Jeremy G. , Gillespie Callan M. , Colbrunn Robb W. , Schlenk Richard , Steinmetz Michael P. , Recinos Pablo F. , Benzel Edward C. , Kshettry Varun R. 
Published: October 2021

OBJECTIVE
The direct lateral approach is an alternative to the transoral or endonasal approaches to ventral epidural lesions at the lower craniocervical junction. In this study, the authors performed, to their knowledge, the first in vitro biomechanical evaluation of the craniovertebral junction after sequential unilateral C1 lateral mass resection. The authors hypothesized that partial resection of the lateral mass would not result in a significant increase in range of motion (ROM) and may not require internal stabilization.

METHODS
The authors performed multidirectional in vitro ROM testing using a robotic spine testing system on 8 fresh cadaveric specimens. We evaluated ROM in 3 primary movements (axial rotation [AR], flexion/extension [FE], and lateral bending [LB]) and 4 coupled movements (AR+E, AR+F, LB + left AR, and LB + right AR). Testing was performed in the intact state, after C1 hemilaminectomy, and after sequential 25%, 50%, 75%, and 100% C1 lateral mass resection.

RESULTS
There were no significant increases in occipital bone (Oc)–C1, C1–2, or Oc–C2 ROM after C1 hemilaminectomy and 25% lateral mass resection. After 50% resection, Oc–C1 AR ROM increased by 54.4% (p = 0.002), Oc LB ROM increased by 47.8% (p = 0.010), and Oc–C1 AR+E ROM increased by 65.8% (p < 0.001). Oc–C2 FE ROM increased by 7.2% (p = 0.016) after 50% resection; 75% and 100% lateral mass resection resulted in further increases in ROM.

CONCLUSIONS
In this cadaveric biomechanical study, the authors found that unilateral C1 hemilaminectomy and 25% resection of the C1 lateral mass did not result in significant biomechanical instability at the occipitocervical junction, and 50% resection led to significant increases in Oc–C2 ROM. This is the first biomechanical study of lateral mass resection, and future studies can serve to validate these findings.
Anti-proliferative activity of yerba mate (Ilex paraguariensis) aqueous extracts on human colorectal cancer cell lines
Authors: Jason Saleh Ali , Othman Leen , Elchoueiry Michel , Ghanem Rita , Bazzi Samer , El-Sabban Marwan , Abdel-Massih Roula M 
Published: October 2021

Background: Yerba mate, a popular, tea-like beverage prepared from the dried leaves of Ilex paraguariensis, is widely consumed, and has several reported health benefits. Compared with other herbal teas, the effect of yerba mate on human cells in the context of cancer has not been extensively studied. The method of extraction of bioactive compounds from the yerba mate leaves plays an important role in its effect on cancer cells. Methods: In this study we assessed the viability, anti-proliferative, and apoptotic effect of the aqueous yerba mate extract, prepared using the same conditions employed for consumption, on different human colorectal cancer cell lines (Caco-2, HT-29, and HCT116) and on the non-tumorigenic human colon epithelial cell line (NCM460).Results: Cytotoxicity of aqueous yerba mate extract was studied and a dose-dependent decrease in viability was observed in all the tested cell lines. At 24 hrs., viability decreased to 19.7% with Caco-2 cells, 2.7% with HCT116, and 8.4% with HT-29 cells at a concentration of 4.8 mg/mL of yerba mate extract. The effect was less prominent on the NCM460 cell line where the viability of cells at the same concentration was 65.2%. Yerba mate extract also showed concentration-dependent anti-proliferative effects as determined by the WST-1 proliferation kit. IC50 values ranged between 0.22-0.69 mg/mL at 24 hr for cell lines tested. To study whether cell death was due to apoptosis, Caco-2 cells were stained with Annexin V-FITC assay and an increase in the percentage of late apoptotic Caco-2 cells was observed with yerba mate extract at 0.6-4.8 mg/mL. Cell cycle analysis using DNA content by flow cytometry showed an increase in the percentage of Caco-2 cells in the subG0/G1 phase and the G0/G1 phase after treatment with 2.4 mg/mL extract. Collectively, our data suggest that yerba mate aqueous extract exhibits an anti-proliferative effect on tested cell lines by inducing apoptosis. Conclusions: Yerba mate aqueous extract exhibits a strong anti-proliferative activity against adenocarcinoma cell lines studied and constitutes a promising functional food adjuvant to anti-cancer therapy. Further work is needed to identify active components and mechanisms of action. Keywords: Aqueous extract, yerba mate, anti-proliferative activity, adenocarcinoma cell lines
CLINICAL AND MICROBIOLOGICAL SUBSTANTIATION OF TOPICAL APPLICATION OF SORPTION HYDROPHILIC/HYDROPHOBIC COMPOSITION BASED ON NANOSILICA IN THE TREATMENT OF PATIENTS WITH BURNS
Authors: Chornopyshchuk R.M. , Nagaichuk V.I. , Gerashchenko I.I. , Nazarchuk O.A. , Sidorenkov S.A. , Chepliaka O.M. , Sidorenko L.A. 
Published: October 2021

Relevance.One of the promising methods of treatment of patients with burns is the local use of sorption agents with antimicrobial properties.
Objective: experimental study of antimicrobial properties and clinical evaluation of the effectiveness of local use of a new sorption composition based on nanosilica in the complex treatment of patients with burns.
Materials and methods. The suggested sorption nanocomposition included highly dispersed silicon dioxide, polymethylsiloxane, decamethoxine, metronidazole.
The experimental study involved the study of the antimicrobial properties of the sorption nanocomposition and antimicrobial agents which are part of it.
The clinical material consisted of the results of examination of 42 patients with IIab-III degree burns with an area of 10-30% of the body surface. Depending on the tactics of local treatment, patients were divided into 2 groups: after early necrectomy, xenodermoplasty, the wound surface of the patients in the main group (n = 20) was treated with a solution of decamethoxine in combination with the same sorption powder. Treatment in the comparison group (n = 22) was similar except the use of sorption drugs. The examination included visual inspection of the injured area in combination with microbiological monitoring of the wound contents on the 3rd,7th,14th day.
Results. The obtained results confirmed the sufficient antimicrobial potential of the studied sorption nanocomposition, the properties of which are not inferior to the existing antiseptics for museum and clinical strains of microorganisms and fungi.
Signs of a more favorable wound healing process of the patients in the main group were observed: faster wound cleaning, less inflammatory reactions and much shorter preparation of wounds for grafting.
Conclusions. The obtained results convincingly indicate the effectiveness of a multicomponent composite based on nanosilica with antimicrobial components in a comprehensive treatment of patients with burns.
Expression pattern of MRPS18 family genes in gliomas
Authors: Kovalevska L.M. , Malysheva T.A. , Kalman S.S. , Rozumenko A.V. , Verbova L.V. , Rozumenko V.D. , Kashuba E.V. 
Published: October 2021

Summary. Aim: To assess expression patterns of MRPS18 family genes in glioblastoma tissues and glioma cell lines. Materials and Methods: Expression of MRPS18 family genes was analyzed by quantitative polymerase chain reaction in glioma cell lines and glioblastoma specimens. A bioinformatic analysis of the publicly available data on the expression of these genes was also provided. Results: The genes of MRPS18 family show different expression patterns in glioblastomas and glioma cell lines. The highest levels of expression were found for MRPS18-2 at mRNA and protein levels in both glioblastomas and glioma cell lines; the lowest — for MRPS18-1 at mRNA level. Conclusions: The elevated levels of relative expression of the MRPS18-2 gene are characteristic for glioma tumor tissues and cell lines.
ESR1 gene variants affect FSHR-depended risk of fibrocystic mastopathy in infertile women
Authors: Kornatska A.G. , Rossokha Z.I. , Fishchuk L.Ye. , Dubenko O.D. , Medvedieva N.L. , Flaksemberg М.А. , Chubei G.V. , Popova O.F. , Gorovenko N.G. 
Published: October 2021

Summary. Background: The infertile women have an increased risk of developing benign and malignant tumors, in particular, breast cancer. Most studies have examined the role of gene variants in the risk of developing breast cancer, but there is little evidence of genetic risk factors for benign tumors. Aim: To assess the combined genetic risk of developing mastopathy in women with FSHR (rs6165, rs6166) and ESR1 (rs9340799, rs2234693) gene variants. Materials and Methods: The study included 87 infertile women (45 with concomitant fibrocystic mastopathy and 42 without mastopathy). Results: For rs9340799 and rs2234693 variants of the ESR1 gene, we did not find any significant differences in the distribution of genotypes in infertile women with or without mastopathy. In patients with mastopathy, there was a reliable increase in the frequency of 307Ala/Ala and 680Ser/Ser genotypes of FSHR gene (χ2 = 6.39, p = 0.012, OR = 4.49 (1.48–13.65)) as compared to patients without mastopathy. In the presence of 307Thr/Thr and 680Asn/Asn genotypes of the FSHR gene, a 4.88-fold reduction of mastopathy risk (χ2 = 8.06, p = 0.005, OR = 0.21(0.07–0.59)) was observed. The frequency of the FSHR and the ESR1 genotypes combinations — 307Thr/Thr+680Asn/Asn+351AG+397TC was significantly decreased in patients with mastopathy. Conclusions: Our study did not find an association of ESR1 gene variants with the risk of developing of mastopathy in infertile women although heterozygous variants of the ESR1 gene enhanced the “protective” effect of FSHR gene variants and reduced the risk of mastopathy.
Tumor Neuroectodérmico Primitivo na Infância: Relato de 13 Casos e Revisão da Literatura
Authors: Costa Cecília M. L. , Rondinelli Patricia , Camargo Beatriz de 
Published: October 2021

Proposta: A proposta desse estudo é a análise retrospectiva dos pacientes com diagnóstico de tumor neuroectodérmico primitivo (PNET), admitidos no Departamento de Pediatria do Hospital do Câncer no periodo de 1989 a 1996. Pacienttes e métodos: Todos os 13 pacientes portadores de PNET foram analisados retrospectivamente utilizando uma ficha para obtenção dos dados epidemiológicos clínicos, terapêuticos e seguimento de cada paciente. Resultados: A cirurgia foi a primeira abordagem terapêutica em 2 pacientes. Quimioterapia foi administrada em 12 pacientes e radioterapia em 2. Dos 13 pacientes 5 estão vivos em seguimento médio de 48 meses após o término do tratamento. Conclusão: A análise dos 13 pacientes com PNET e revisão da literatura demonstram a agressividade desse tumor. Devido a raridade dessa patologia, estudos multiinstitucionais, podem ter papel importante para análise de fatores prognósticos e consequente abordagem terapêutica.
Study of polysynaptic reflex excitability and somatosensory evoked potentials in patients with myofascial pain syndrome
Authors: Safiullina A. A. , Safiullina G. I. , Yakupov R. A. 
Published: October 2021

Introduction. Myofascial pain syndrome (MFPS) is one of the most common pathologies of the musculoskeletal system that causes chronic pain. This type of pain reaches its peak in middle-aged people; women get sick 2,5 times more often than men. The disease leads to significant disability; it is not only a medical but also a social problem. Atthe same time, a number of authors still note the low clinical efficacy of the applied therapy regimens. In this situation there is in demand the further study of the MFPS pathogenesis, in particular, the clinical and electroneurophysiological characteristics of this pathology, in order to search for new, including pathogenetically proved, treatment methods.The aim of the research was to study the relationship between the indicators of the blinking reflex and somatosensory evoked potentials in patients with active and latent forms of MFPS.Materials and methods. In order to study the neurophysiological aspects of MFPS, a comprehensive clinical and electroneurophysiological examination was carried out in 92 patients of working age, including 78 women and 14 men, whose average age was 48,1±9,3) years, suffering from MFPS of the scapular area. The patients were subdivided into subgroups depending on the prevalence and severity of pain manifestations: the first subgroup — with an active form (58 people, 63 %), the second subgroup — with a latent form of MFPS (34 people, 37 %). The control group was formed from conditionally healthy volunteers in the amount of 35 people, comparable to the main group by age and sex.Results. The comparing of the study results, taking into account polysynaptic reflex excitability and the data of somatosensory evoked potentials, revealed the significant positive correlations of the indicators. The obtained results allowed to assess the excitability of spinal cord motor neurons as well as suprasegmental structures in MFPS, involved in the implementation of pain syndrome.Conclusion. The use of clinical electroneurophysiology methods makes it possible to assess the functional state of the CNS structures involved in the analysis of nociceptive afferentation. In the active form of MFPS, an increase in the excitability of stem and thalamic structures was mainly determined, which could potentially indicate the activation of adaptive processes on the one hand, and on the other hand, the predisposition of neuronal networks to a state of arousal. In the latent form of MFPS, a multidirectional change in reflex excitability was observed.
Box-Behnken Design for Optimization on Biodiesel Production from Palm Oil and Methyl Acetate using Ultrasound Assisted Interesterification Method
Authors: Mahfud Mahfud , Ansori Ansori 
Published: October 2021

Energy demand is currently increasing in line with technological and economic developments, but not accompanied by an increase in energy reserves. So we need another alternative energy that can be renewed, namely biodiesel. Biodiesel has been produced commercially through the transesterification from vegetable oil with methanol using catalyst that produces esters and glycerol. The formation of glycerol which is by-product can reduce its economic value, so it needs to be done the separation process. Therefore, a new route is proposed in this study, namely the interesterification reaction (non-alcoholic route) using methyl acetate as an alkyl group supplier and potassium methoxide catalyst. The superiority of the product produced by the interesterification reaction is biodiesel with triacetin byproducts which have an economical value and can be added to biodiesel formulations because of their solubility so that no side product separation process is needed. To increase the yield of biodiesel and the interesterification rate, the ultrasound method was used in this study. To optimize the factors that affect the interesterification reaction (molar ratio of methyl acetate to oil, catalyst concentration, temperature, and interesterification time), the Box-Behnken design (BBD) is used. Optimal operating conditions to produce the yields of biodiesel of 98.64 % are at molar ratio of methyl acetate to palm oil of 18.74, catalyst concentration of 1.24 %, temperature of 57.84 °C, and interesterification time of 12.69 minutes.
อุปกรณ์ช่วยสำหรับกิจวัตรประจำวันในผู้สูงอายุหลังกระดูกสะโพกหัก
Authors: กองเกตุใหญ่ พนินทร , ธนาภาธิวัตถ์ วิภาวี , เดชพระธรรม ปิยะภัทร , อนุจา วาสนา 
Published: October 2021

กระดูกสะโพกหักเป็นภาวะกระดูกหักที่พบได้บ่อยในผู้สูงอายุ หลังได้รับการผ่าตัด ผู้สูงอายุจะมีข้อจำกัดในการทำกิจกรรมต่าง ๆโดยเฉพาะการทำกิจวัตรประจำวัน ส่งผลให้ผู้สูงอายุและผู้ดูแลเกิดความเครียดและวิตกกังวล การฟื้นฟูทางกิจกรรมบำบัดในผู้ป่วยกลุ่มนี้จะแนะนำการใช้อุปกรณ์ดัดแปลงเพื่อช่วยเพิ่มประสิทธิภาพในการทำกิจวัตรประจำวันด้วยตนเองหลังจากการผ่าตัด โดยช่วยให้มีการจัดท่าทางที่ถูกต้องและลดการเคลื่อนหลุดของกระดูกสะโพกเนื่องจากการเคลื่อนไหวในท่าที่ไม่เหมาะสม บทความนี้จึงนำเสนอแนวทางในการนำอุปกรณ์ดัดแปลงแบบต่าง ๆ มาใช้ในการทำกิจวัตรประจำวันในผู้สูงอายุหลังกระดูกสะโพกหัก ซึ่งอุปกรณ์ดัดแปลงเหล่านี้มีทั้งแบบที่มีจำหน่ายทั่วไปหรืออาจทำจากวัสดุที่มีจำหน่ายในท้องตลาด โดยผู้เรียบเรียงบทความได้อธิบายถึงวิธีการประดิษฐ์อุปกรณ์บางชนิด เพื่อให้ผู้ที่สนใจสามารถจัดหาและนำมาประดิษฐ์ได้ด้วยตนเองต่อไป
The Perceptions of Roles and Understanding about Forensic Evidence and Crime Scene Preservation of Thai Paramedics
Authors: Huabbangyang Thongpitak , Kulnides Narong 
Published: October 2021

Background: Paramedicine is a new profession in Thailand. The most important role is to provide pre-hospital advanced life support for emergency patients. So, they often need to assist the injured at crime scenes. However, many do not know their role or how to preserve the forensic evidence and crime scene. Currently in Thailand, their role in forensic evidence and crime scene preservation (FECSP) is unclear. Hence, the management of the evidence and the crime scene depends on individual knowledge and experience.
Objectives: 1. To study the perceptions of roles and understanding of FECSP of Thai paramedics. 2. To study the problems, obstacles, and solutions for development of Thai paramedics FECSP practices. 3. To study the factors affecting the perceptions of roles and understanding of FECSP in Thai paramedics.Method: Thai paramedics data over the country registered with National Institute for Emergency Medicine (NIEMS) during 1st March – 31st March 2021 was collected in this cross-sectional survey. The questionnaires were sent as Google forms to them by e-mail.
Results: 382 questionnaires were sent, and 281 responses (74%) were obtained. Most were female (61.9%). The average age was 26.09±4.44 years. The most common crime scene experienced was traffic accidents. Most had never had additional training related to forensic science. The perceptions of roles and understanding about FECSP were at the highest level. The most common problem and hindrance about FECSP was no FECSP law and the most common solution for improvement of the FECSP was the standard FECSP guideline development. Hospital level was found to be a factor related to the perceptions of roles of FECSP in Thai paramedics. Average score of a cohort who worked at university hospital was higher than those working at tertiary hospitals 0.220 (B = -0.220 , p-value = 0.018). Additionally, hospital level was also a factor concerning the understanding about FECSP of Thai paramedics. The average score of cohorts who worked at university hospitals was greater than those working in primary or secondary hospitals 0.197 (B = -0.197, p-value = 0.022).
Conclusion: The paramedics had the perception of the roles and understanding about FECSP at the highest level. Hospital level was a significant factor related to the perception of the roles and understanding about FECSP. Relevant health institutes should develop standard guidelines and promote FECSP training.
López Morales, F.J. (coord.) El patrimonio cultural inmaterial: usos sustentables del patrimonio
Authors: Marín Prieto José Miguel 
Published: October 2021

Reseña del libro El patrimonio cultural inmaterial: usos sustentables del patrimonio, coordinado por D.Francisco Javier López Morales y publicado por el Instituto Nacional de Antropología e Historia, Tlaxcala, México, año 2017.
KARAKTERISTIK KIMIA DAN ORGANOLEPTIK PRODUK STIK DENGAN SUBSTITUSI DAGING IKAN GABUS (Channa striata)
Authors: Fera Feti , Asnani Asnani , Asyik Nur 
Published: October 2021

ABSTRACT The aim of this study was to determine the effect of fish meat substitution on organoleptic and chemical compound of stick product. This study used a Randomized Complete Design (RCD) consist of four treatments such as P0 as control (0% fish meat : 100% wheat flour); P1 (30% fish meat : 70% wheat flour); P2 (40% fish meat : 60% wheat flour); P3 (50% fish meat : 50% wheat flour). Result showed that the highest value on sensory analysis was P3 with rather good category. The highest value of sensory analysis was P3, including colour (yellow-brownish), aroma (fishy), chrunciness (crunchy) and juiciness (very juicy). The highest value of protein and carbohydrate compound was P3 39.13% and 71.56% respectively. Keywords: Crackers, mackerel scad fish (Decapterus spp.), seaweed (E. cottoni), organoleptic test, chemical testABSTRAKPenelitian ini bertujuan untuk mempelajari pengaruh substitusi daging ikan terhadap nilai organoleptik dan komposisi kimia produk stik. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) yang terdiri dari empat perlakuan yaitu P0 (kontrol) (0% daging ikan: tepung terigu 100%), P1 (daging ikan 30% : tepung terigu 70%), P2 (daging ikan 40% : tepung terigu 60%) dan P3 (daging ikan 50% : tepung terigu 50%). Berdasarkan hasil penelitian produk stik yang disubstitusikan dengan daging ikan gabus diperoleh perlakuan tertinggi uji sensorik diperoleh pada perlakuan P3 dengan penilain panelis yang cukup baik. Rerata uji sensorik tertinggi terdapat pada perlakuan P3 meliputi warna (kuning kecoklatan), aroma (beraroma ikan), rasa (terasa ikan), kerenyahan (renyah), dan rasa gurih (sangat gurih). Komposisi kimia protein dan karbohidrat tertinggi pada produk stik ditemukan pada perlakuan P3 masing-masing sebesar 39,13 dan 71,56%.Kata kunci: Eucheuma cottonii, Ikan layang (Decapterus spp), Kerupuk, uji organoleptik, uji kimia