bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comparing synaptic proteomes across seven mouse models for autism reveals molecular subtypes and deficits in Rho GTPase signaling
Authors: Carbonell Abigail U. , Freire-Cobo Carmen , Deyneko Ilana V. , Erdjument-Bromage Hediye , Clipperton-Allen Amy E. , Rasmusson Randall L. , Page Damon T. , Neubert Thomas A. , Jordan Bryen A. 
Published: February 2021

AbstractImpaired synaptic function is a common phenotype in animal models for autism spectrum disorder (ASD), and ASD risk genes are enriched for synaptic function. Here we leverage the availability of multiple ASD mouse models exhibiting synaptic deficits and behavioral correlates of ASD and use quantitative mass spectrometry with isobaric tandem mass tagging (TMT) to compare the hippocampal synaptic proteomes from 7 mouse models. We identified common altered cellular and molecular pathways at the synapse, including changes in Rho family small GTPase signaling, suggesting that it may be a point of convergence in ASD. Comparative analyses also revealed clusters of synaptic profiles, with similarities observed among models for Fragile X syndrome (Fmr1knockout), PTEN hamartoma tumor syndrome (Ptenhaploinsufficiency), and the BTBR+ model of idiopathic ASD. Opposing changes were found in models for cortical dysplasia focal epilepsy syndrome (Cntnap2knockout), Phelan McDermid syndrome (Shank3InsG3680), Timothy syndrome (Cacna1cG406R), and ANKS1B syndrome (Anks1bhaploinsufficiency), which were similar to each other. We propose that these clusters of synaptic profiles form the basis for molecular subtypes that explain genetic heterogeneity in ASD despite a common clinical diagnosis. Drawn from an internally controlled survey of the synaptic proteome across animal models, our findings support the notion that synaptic dysfunction in the hippocampus is a shared mechanism of disease in ASD, and that Rho GTPase signaling may be an important pathway leading to disease phenotypes in autism.
Assessment of immunogenicity and protective efficacy of ZyCoV-D DNA vaccine candidates in Rhesus macaques against SARS-CoV-2 infection
Authors: Yadav Pragya D , Kumar Sanjay , Agarwal Kshitij , Jain Mukul , Patil Dilip R , Maithal Kapil , Mathapati Basavaraj , Giri Suresh , Mohandas Sreelekshmy , Shete Anita , Sapkal Gajanan , Patil Deepak Y , Dey Ayan , Chandra Harish , Deshpande Gururaj , Gupta Nivedita , Nyayanit Dimpal , Kaushal Himanshu , Sahay Rima , Tripathy Anuradha , Jain Rajlaxmi , Kumar Abhimanyu , Sarkale Prasad , Baradkar Shreekant , Rajanathan Chozhavel , Raju Hari Prasad , Patel Satish , Shah Niraj , Dwivedi Pankaj , Singh Dharmendra , Abraham Priya 
Published: February 2021

AbstractVaccines remain the key protective measure to achieve herd immunity to control the disease burden and stop COVID-19 pandemic. We have developed and assessed the immunogenicity and protective efficacy of two formulations (1mg and 2mg) of ZyCoV-D (a plasmid DNA based vaccine candidates) administered through Needle Free Injection System (NFIS) and syringe-needle (intradermal) in rhesus macaques with three dose vaccine regimens. The vaccine candidate 2mg dose administered using Needle Free Injection System (NFIS) elicited a significant immune response with development of SARS-CoV-2 S1 spike region specific IgG and neutralizing antibody (NAb) titers during the immunization phase and significant enhancement in the levels after the virus challenge. In 2 mg NFIS group the IgG and NAb titers were maintained and showed gradual rise during the immunization period (15 weeks) and till 2 weeks after the virus challenge. It also conferred better protection to macaques evident by the viral clearance from nasal swab, throat swab and bronchoalveolar lavage fluid specimens in comparison with macaques from other immunized groups. In contrast, the animals from placebo group developed high levels of viremia and lung disease following the virus challenge. Besides this, the vaccine candidate also induced increase lymphocyte proliferation and cytokines response (IL-6, IL-5).The administration of the vaccine candidate with NFIS generated a better immunogenicity response in comparison to syringe-needle (intradermal route). The study demonstrated immunogenicity and protective efficacy of the vaccine candidate, ZyCoV-D in rhesus macaques.
Diffusion of the disordered E-cadherin tail on β-catenin
Authors: Wiggers Felix , Wohl Samuel , Dubovetskyi Artem , Rosenblum Gabriel , Zheng Wenwei , Hofmann Hagen 
Published: February 2021

AbstractSpecific protein interactions typically require well-shaped binding interfaces. Here, we report a cunning exception. The disordered tail of the cell-adhesion protein E-cadherin dynamically samples a large surface area of the proto-oncogene β-catenin. Single-molecule experiments and molecular simulations resolve these motions with high resolution in space and time. Contacts break and form within hundreds of microseconds without dissociation of the complex. A few persistent interactions provide specificity whereas unspecific contacts boost affinity. The energy landscape of this complex is rugged with many small barriers (3 – 4kBT) and reconciles specificity, high affinity, and extreme disorder. Given the roles of β-catenin in cell-adhesion, signalling, and cancer, this Velcro-like design has the potential to tune the stability of the complex without requiring dissociation.
Revision of commonly accepted Warburg mechanism of cancer development. Redox-sensitive mitochondrial cytochromes in breast and brain cancers by Raman imaging
Authors: Abramczyk Halina , Surmacki Jakub Maciej , Brozek-Pluska Beata , Kopeć Monika 
Published: February 2021

AbstractBackgroundWe studied oncogenic processes that characterize human breast cancer (infiltrating ductal carcinoma (IDC)) and human brain tumors: glioma, astrocytoma and medulloblastoma based on the quantification of cytochrome redox status by exploiting the resonance-enhancement effect of Raman scattering.MethodsWe used Raman imaging to monitor changes in the redox state of the mitochondrial cytochromes in ex vivo human brain and breast tissues surgically resected specimens of human tissues and in vitro human brain cells of normal astrocytes (NHA), astrocytoma (CRL-1718), glioblastoma (U87-MG) and medulloblastoma (Daoy), and human breast cells of normal cells (MCF 10A), slightly malignant cells (MCF7) and highly aggressive cells (MDA-MB-231) by means of Raman microspectroscopy at 532 nm.ResultsWe visualized localization of cytochromes by Raman imaging in the major organelles in cancer cells. We demonstrated that the “redox state Raman marker” of the ferric low spin heme in cytochrome c at 1584 cm−1can serve as a sensitive indicator of cancer aggressiveness. We compared concentration of reduced cytochrome c and the grade of cancer aggressiveness in cancer tissues and single cells and specific organelles in cells: nucleous, mitochondrium, lipid droplets, cytoplasm, and membrane.ConclusionsWe found that the concentration of reduced cytochrome c becomes abnormally high in human brain tumors and breast cancers in human tissues. Our results suggest that the mechanisms controlling the electron transport chain are spectacularly deregulated in cancers and indicate that electron transport, organized in terms ofelectronegativity, is inhibited between complex III and cytochrome c for isolated cells in vitro and between cytochrome c and complex IV in brain and breast tissues. The results provide evidence that the extracellular matrix and interactions with cell microenvironment play an important role in the mechanisms controlling the electron transport chain by cytochrome c. Our results reveal the universality of Raman vibrational characteristics of mitochondrial cytochromes in metabolic regulation in cancers that arise from epithelial breast cells and brain glial cells.
Optimization of pre-oxidation time for corrosion resistance of cobalt based coating alloy in mixed sulphates
Authors: Rehman Abdul , Bidabadi Mohammad Hassan Shirani , Yang Liang , Yu Zheng , Hao Chen , Zhang Chi , Yang Zhigang 
Published: February 2021

Purpose
This study aims to optimise the effect of pre-oxidation on hot corrosion behaviour of Tribaloy T-900 at 900 °C in mixed Na2SO4 and K2SO4.

Design/methodology/approach
Prior to hot corrosion experiment, pre-oxidation treatments were carried in ambient air at 900 °C for 1, 5 and 10 h, respectively. The hot corrosion experiments were performed in a box type furnace at 900 °C. Both surfaces of specimens were brushed with saturated salt solution of 75 wt.% Na2SO4 + 25 wt.% K2SO4. After brushing, the salt-coated specimens were placed in electric stove to ensure drying of salt. After drying, presence of 3 mg/cm2 salt on specimen’s surface was ensured through weighting.

Findings
The 1-h pre-oxidation treatment prior to hot corrosion showed superior hot corrosion resistance against molten salt attack. An optimum pre-oxidation time of 1 h helped timely formation of protective Cr2O3 layer and inhibited the formation of less stable and porous surface oxides of Ni and Mo during hot corrosion.

Originality/value
Pre-oxidation effect on hot corrosion behaviour of refractory metal (such as Mo in investigated alloy) containing alloy has never been reported previously. Refractory metals oxide (e.g. MoO3) could transform the corrosion phenomena to catastrophic failure through acidic fluxing.
Road infrastructure development under PPP model in India: a credit rating perspective
Authors: Koul Puneet , Verma Piyush , Arora Lalit 
Published: February 2021

PurposeThe study analyzes significant parameters defining the credit worthiness, economic viability and managerial efficiency of special purpose vehicles (SPVs) of infrastructure development firms engaged in the execution of road projects under PPP model in India.Design/methodology/approachThe study is based on a comprehensive review of credit rating reports of major rating agencies. In particular, 18 special purpose vehicles (13 BOT-toll–based and 5 BOT-annuity–based road projects) during the period 2010–2019 were considered to conduct a comparative analysis of their rating progression. Considering both financial as well as nonfinancial parameters, their segregation was done on the basis of strengths, constraints and key rating sensitivities influencing the ratings of SPVs involved in road projects under PPP model.FindingsPromoters' credibility emerged as an important factor affecting PPP credit ratings. Other prominent factors included nature of stretch and regulatory terms and conditions and the project's potential to generate cash flows. Inability of PPP projects to generate the projected levels of toll collections was a major constraint and hampered ratings over time. Growth in traffic was a key sensitive area in a toll-based project. Interestingly, despite the fixed nature of revenues, BOT (annuity) projects were impacted by rating changes.Research limitations/implicationsFewer sample projects (for which the data were available) was a constraint. Future research could consider larger data sets to provide deeper insights. An examination of credit rating parameters using rating reports of projects in other developing nations could provide meaningful implications. The findings of this research however cannot be undermined as the study bridges a gap in existing literature pertaining to the examination of PPP model from a credit rating perspective.Practical implicationsThis study would guide project developers, government agencies and awarding agencies of PPP road projects to anticipate the challenges and take adequate steps to mitigate them.Originality/valueResearch in the area of PPP projects is skewed toward risk assessment with respect to financial parameters. The present study emphasizes the rating framework of SPVs. Comprehensive examination of factors affecting project ratings in the form of projects' strengths, constraints and sensitivities would provide inputs to academics and researchers.
Big data empowered agility for dynamic, volatile, and time-sensitive service industries: the case of tourism sector
Authors: Stylos Nikolaos , Zwiegelaar Jeremy , Buhalis Dimitrios 
Published: February 2021

PurposeDynamic, volatile, and time-sensitive industries, such as tourism, travel and hospitality require agility and market intelligence to create value and achieve competitive advantage. The aim of the current study is to examine the influence of big data (BD) on the performance of service organizations and to probe for a deeper understanding of implementing BD, based on available technologies.Design/methodology/approachAn ethnographic study was conducted following an abductive approach. A primary qualitative research scheme was used with 35 information technology and database professionals participating in five online focus groups of seven participants each. Analytical themes were developed simultaneously with the literature being revisited throughout the study to ultimately create sets of common themes and dimensions.FindingsBD can help organizations build agility, especially within dynamic industries, to better predict customer behavioral patterns and make tailor-made propositions from the BD. An integrated BD-specific framework is proposed to address value according to the dimensions of need, value, time and utility.Research limitations/implicationsLittle research exists on the key drivers of BD use for dynamic, real-time and agile businesses. This research adds to the developing literature on BD applications to support organizational decision-making and business performance in the tourism industry.Originality/valueThis study responds to scholars’ recent calls for more empirical research with contextual understanding of the use of BD to add value in marketing intelligence within business ecosystems. It delineates factors contributing to BD value creation and explores the impacts on the respective service encounters.
An emotional intelligence model of entrepreneurial coping strategies
Authors: Pathak Saurav , Goltz Sonia 
Published: February 2021

PurposeThe purpose of this article is to recognize emotional intelligence (EI) as a specific emotional competency possessed by entrepreneurs that facilitates their coping with stressors that arise in their day-to-day work. Highlighting the problem-focused and emotion-focused coping strategies employed by entrepreneurs, the paper establishes that EI in entrepreneurs enables the onset of acceptance of the existence of a stressful situation and that of optimism that a stressful situation can be solved.Design/methodology/approachThe paper reviews literature on the topics related to links between EI, entrepreneurial behaviors and entrepreneurial coping. Subsequently, acceptance-avoidance motivation theory is used to posit that entrepreneurs' EI serves as an antecedent that (1) guides the selection of their coping strategies through onset of optimism and acceptance of stressful situation, (2) assists in perceiving those situations as either controllable or uncontrollable and (3) shapes the actual process through which entrepreneurs cope.FindingsThe proposed conceptual model asserts that entrepreneurs' EI as reflected in their abilities to perceive, use, understand and regulate emotions is key to their coping. EI in entrepreneurs assists them in being optimistic about solving a stressful situation and accepting of such situations as well. Thereafter, selection of a problem-focused or emotion-focused coping strategy or both ensues.Originality/valueThe current work offers a conceptual model that highlights the role of entrepreneurs' EI in coping, integrates both the cognitive and affective components of coping and suggests a variety of avenues for future research. This model extends models of coping that categorize coping types to specify the process thought to be involved. Understanding the role of emotional intelligence in coping with stress by entrepreneurs has theoretical and practical implications which are discussed as well.
Interpersonal forgiveness and employee life satisfaction: the role of affect at work
Authors: Dahiya Rinki 
Published: February 2021

Purpose
Amidst the new realities of the modern world, a happy and satisfied workforce has become a necessary requirement for organizations to maintain their competitive edge. While most of the available literature revolves around positive organizational behaviour, there is dearth of research on the influence of interpersonal forgiveness on affect and life satisfaction in employees. Keeping this precept in mind, this study aims at bridging this gap in research by developing and testing a mediation model to examine the link between interpersonal forgiveness to affect and life satisfaction.

Design/methodology/approach
The sample consists of 378 employees working in different Indian manufacturing organizations. To test the hypotheses, structural equation modelling was applied.

Findings
The results reveal that interpersonal forgiveness significantly influences life satisfaction and affect partially mediates this relationship in employees.

Practical implications
The study recommends that organizational development practitioners and human resource professionals focus on forgiveness among co-workers to foster a balanced affect and enhanced life satisfaction by developing and implementing positive psychological interventions and practices. This can help organizations in regulating interpersonal transgressions and conflicts at an early stage and also make the employees happy and satisfied.

Originality/value
This study offers concrete insights into the complicated interplay of affect in the link between interpersonal forgiveness and life satisfaction in the yet underexplored context of Indian organizations.
Aligning individual and organizational R&D goals for self-sustainability: investigating preferences of researchers in selected CSIR laboratories, India
Authors: Kumari Beena , Sahney Sangeeta , Madhukar Anuradha 
Published: February 2021

PurposeThis paper intends to explore the measure for aligning the goals of researchers toward achieving organizational R&D targets. The paper also explores the significance and ordering of R&D outputs and the factors that influence generation of R&D outputs, from the perspective of researchers working in the Indian public sector organizations.Design/methodology/approachData were collected in five Indian R&D laboratories, and weighted average method Spearman correlation coefficient and rank regression were utilized for the analysis.FindingsThe findings indicated that various groups of researchers prefer to target different R&D outputs and not all the factors are considered as equally significant in influencing the generation of R&D outputs. Further, the R&D organization should include preferred real factors while policy making for achieving collaborative efforts toward fulfilling organizational objectives. The set of selected R&D outputs and influencing factors were also ordered according to the average rankings given by the researchers.Practical implicationsThe findings can help R&D managers to identify the expectations of the researchers and include their preferences in R&D Planning. The study could be extended to a larger dataset of researchers working in other government as well as private R&D organizations.Originality/valueHardly any studies were found that explored the preferences of researchers with respect to R&D outputs and influencing factors with respect to the Indian public sector R&D laboratories.
Drivers of sustainable entrepreneurial intentions among university students: an integrated model from a developing world context
Authors: Agu Agu Godswill , Kalu Okwuagwu Okuu , Esi-Ubani Chidadi Obinna , Agu Paul Chinedu 
Published: February 2021

Purpose
The purpose of this study is to integrate and extend two models of entrepreneurial intention to investigate the drivers of sustainable entrepreneurial intention among intermediate undergraduate university students in Nigeria. Specifically, this paper aims to introduce education for sustainable entrepreneurship into the integrated model, thereby fitting the model into the context of sustainable entrepreneurship.

Design/methodology/approach
Data was gathered with the help of a structured questionnaire from 435 students of a university in Nigeria. The students passed through a special entrepreneurship training in which they were educated on the concept and practice of sustainable entrepreneurship. SmartPLS was used to test the proposed structural model.

Findings
The findings revealed that education for sustainable entrepreneurship significantly influences all variables of the integrated model, but has nonsignificant direct influence on sustainable entrepreneurial intention. Sustainable entrepreneurial intention is significantly driven by attitude and propensity to act. Therefore, the inclusion of education for sustainable entrepreneurship into the regression equation adds to its explanatory power.

Originality/value
This study contributes toward understanding of sustainable entrepreneurial intention of intermediate university students in a developing world context – Nigeria. Above all, it is among the few studies that shed light on the strength of education for sustainable entrepreneurship in the formation of sustainable entrepreneurial intention among students. This study proposes integration and extension (by adding education for sustainable entrepreneurship) of the theory of planned behavior and entrepreneurial event model in learning about students’ intentions to engage in sustainable entrepreneurship.
Does agro-processing adoption affect farm income and farm diversification? Empirical evidence from Taiwan
Authors: Lin Lin , Chang Hung-Hao 
Published: February 2021

PurposeThe purpose of this paper is to identify the factors associated with the adoption of agro-processing methods and to estimate their impact on farm income and farm diversification.Design/methodology/approachUsing a large-scale sample of 12,122 special crop farm households drawn from the 2015 Agricultural Census Survey in Taiwan, the semiparametric multivalued treatment effect model was estimated.FindingsThe authors found that agro-processing farm households obtain higher farm incomes than non-agro-processing farm households. Among the agro-processing methods, self-processing generates higher farm income than outsourced-processing. Moreover, farm households that adopt either agro-processing method are more likely to diversify into agritourism and other agribusinesses than non-agro-processing farms.Research limitations/implicationsThe authors could only access data on farm income and not on agro-processing costs. Future studies may address the impact of agro-processing on farm profitability if relevant data are available.Originality/valueVery few studies have examined the relationship between agro-processing, farm income and farm diversification. To the best of the authors’ knowledge, this is one of the first papers to examine the impact of different agro-processing practices on farm income and farm diversification.