bluuebunny/crossref_metadata_2025_split huggingface dataset:
Spatiotemporal Changes of Tissue Glycans Depending on Localization in Cardiac Aging
Authors: Itakura Yoko , Hasegawa Yasuko , Kikkawa Yurika , Murakami Yuina , Nagai-Okatani Chiaki , Sasaki Norihiko , Umemura Mariko , Takahashi Yuji , Kimura Tohru , Kuno Atsushi , Ishiwata Toshiyuki , Toyoda Masashi 
Published: October 2021

Abstract
Heart failure is caused by various factors, making its underlying pathogenic mechanisms difficult to identify, and tends to worsen over time. Early diagnosis of cardiovascular disease is the key for treatment to promote healthy life. To detect the structural and functional molecular changes associated with cardiovascular disease, we focused on glycans, which reflect the type and state of cells. We investigated glycan localization in the cardiac tissue of normal mice and their alterations during aging using an evanescent-filed lectin microarray, a technique based on lectin-glycan interaction, and lectin staining. The glycan profiles in the left ventricle showed differences between the luminal side (medial) and the wall side (lateral) region. The former area was characterized by the presence of sialic acid residues.Moreover, age-related changes in glycan profiles were observed earlier in the medial region. The difference in the age-related decrease of a-galactose stained with griffonia simplicifolia lectin-IB4 in different region of the leftventricle suggested spatiotemporal changes in microvessels. The glycan profile, which retains diverse glycan structures, is supported by many cell populations and maintains cardiac function. Glycan localization and changes are expected to be developed as a marker of the signs and symptoms of heart failure in the future.
177Lu-DOTA-0-Tyr3-Octreotate Infusion Modeling for Real-Time Detection and Characterization of Extravasation During PRRT
Authors: Christophe Mazzara , Salvadori Julien , Ritzenthaler Florian , Martin Simon , Porot Clémence , Imperiale Alessio 
Published: October 2021

Abstract
Purpose.

Given the recent and rapid development of peptide receptor radionuclide therapy (PRRT), increasing emphasis should be placed on the early identification and quantification of therapeutic radiopharmaceutical (thRPM) extravasation during intravenous administration. Herein, we provide an analytical model of 177Lu-DOTA0-Tyr3-octreotate (Lutathera®) infusion for real-time detection and characterization of thRPM extravasation.
Methods.

For 33 Lutathera®-based PRRT procedures using the gravity infusion method, equivalent dose rates (EDRs) were monitored at the patient’s arm. Models of flow dynamics for nonextravasated and extravasated infusions were elaborated and compared to experimental data through an equivalent dose rate (EDR) calibration. Nonextravasated infusion was modeled by assuming constant volume dilution of 177Lu activity concentration in the vial and Poiseuille-like laminar flow through the tubing and patient vein. Extravasated infusions were modeled according to their onset times by considering elliptically shaped extravasation with different aspect ratios.
Results.

Over the 33 procedures, the peak of the median EDR was reached 14 min after the start of the infusion with a value of 450 uSv/h. On the basis of experimental measurements, 1 mSv/h was considered the empirical threshold for Lutathera® extravasation requiring cessation of the infusion and start again with a new route of injection. According to our model, the concentration of extravascular activity was directly related to the time of extravasation onset and its duration, a finding inherent in the gravity infusion method. This result should be considered when planning therapeutic strategy in the case of RPM extravasation because the local absorbed dose for β-emitters is closely linked to activity concentration. For selected EDR values, charts of extravasated activity, volume, and activity concentration were computed for extravasation characterization.
Conclusion.

We proposed an analytical model of Lutathera® infusion and extravasation (gravity method) based on EDR monitoring. This approach could be useful for the early detection of thRPM extravasation and for the real-time assessment of activity concentration and volume accumulation in the extravascular medium.
Perinatal Environmental Health Education Intervention to Reduce Exposure to Endocrine Disruptors: The PREVED Project
Authors: OUAZZANI HOURIA EL , Fortin Simon , Venisse Nicolas , Dupuis Antoine , Rouillon Steeve , Cambien Guillaume , Gourgues Anne-Sophie , Pierre-Eugène Pascale , Rabouan Sylvie , MIGEOT Virginie , Albouy-Llaty Marion 
Published: October 2021

Abstract
BackgroundEnvironmental health promotion interventions may reduce endocrine disruptor (ED) exposure. PREVED (PREgnancy, preVention, Endocrine Disruptors) project was developed to improve knowledge, to enhance risk perception and to change exposure behavior. Our objective was to present the phases of PREVED project using the RE-AIM method.MethodsPREVED intervention consisted of 3 workshops during pregnancy. Reach, adoption, and implementation phases were assessed with qualitative studies. Efficacy study consisted of a three-arm randomized controlled trial (RCT) on 268 pregnant women: i) control group (leaflet) ii) intervention group in neutral location iii) intervention group in contextualized location. The main outcome was the percentage evolution of participants who reported consuming canned food. Secondary outcomes were evolution of psycho-social scores, evolution of ED presence in urine and ED presence in colostrum. ResultsThe intervention adoption was centered on upper-privileged women, but implementation assessment showed that key features (highly practical intervention) seemed to be carried out and had initiated some behavior changes. A total of 268 pregnant women participated in the intervention and 230 on randomized controlled trial (control group: 86 and intervention groups: 172). We found no significant differences in consumption of canned food and in percentage of women having a decrease of bisphenol A or parabens in urine, but we found significant increase in the evolution of risk perception score and overall psychosocial score in intervention groups (respectively: +15.73 control versus +21.03 intervention, p=0.003 and +12.39 versus +16.20, p=0.02). We found a significant difference in percentage of women with butylparaben detection between control group and intervention groups (13% versus 3%, p=0.03). ConclusionPREVED intervention is the first intervention research dedicated to perinatal environmental health education in France. By sharing know-how/experience in a positive non-alarmist approach, it improved risk perception, which is a key to behavior change aiming to reduce perinatal ED exposure. Including women in precarious situations stay a major issue.Trial registration number: Retrospectively registered on 31 July 2017 (when the first participant was enrolled in this non-drug intervention, ClinicalTrials.gov was centered in therapeutic trials): NCT03233984 - URL: https://clinicaltrials.gov/ct2/show/NCT03233984
The First Reported Case of Hemoptysis After Silicone Gel Breast Implants
Authors: Zhuo Guang Ying , Zhang Pei Yong , Xiang Tao 
Published: October 2021

Abstract
Background: Hemoptysis after silicone breast prosthesis surgery has not been reported so far, and the mechanism is unclear. Case presentation: A 25-year-old young woman presented hemoptysis after silicone breast prosthesis replacement surgery. CTA revealed diffuse interstitial, alveolar, and terminal bronchial exudate changes in absent of pulmonary embolism. Noninvasive ventilator assisted breathing, combined with atomized inhalation of budesonide 2ml every 8h and intravenous infusion of hydrocortisone 100mg every 12h. Chest CT improved significantly 3 days later, and discharged 10 days later. Conclusions: Diffuse alveolar hemorrhage might be the cause of hemoptysis after silicone breast augmentation, and glucocorticoid treatment is very effective. However, if pulmonary imaging suggested diffuse pulmonary hemorrhage, BAL and lung biopsy should be performed to confirm the clinical diagnosis before treatment.
Effects of Risk Factors for Pregnancy Outcomes of Subsequent Pregnancy Associated with Gestational Diabetes Mellitus: A Retrospective Cohort Study in China.
Authors: Xu Weijiao , Xu Xia , Guo Yanni , Liang Jie , Yan Jianying 
Published: October 2021

Abstract
BackgroundSince the implementation of the three‑child policy in China, the number of high-risk pregnant women has increased, causing serious challenges to health care during pregnancy. In this article, we aimed to investigate the impact of several risk factors for maternal and neonatal outcomes in pregnancies complicated by gestational diabetes mellitus (GDM) and recurrent GDM to formulate a management strategy to minimize the effect of risk factors for gestational diabetes. ResultsPre-pregnancy body mass index (BMI) and gestational weight gain affect maternal and child outcomes in the first and second onset of GDM. Pregnancy interval and fasting blood glucose in early pregnancy influence maternal and child outcomes of recurrent GDM. Maternal lipid levels during early pregnancy have a marked influence on neonatal outcomes in recurrent GDM.ConclusionsOn the basis of this result, weight management should be closely monitored before and during pregnancy. For planning of the second pregnancy with a previous history of GDM, a reasonable time between pregnancies is ideal. Moreover, in the next pregnancy, control of fasting blood glucose and lipid levels during the first trimester is necessary to improve both maternal and child outcomes.
miR-196a-5p Promoted the Osteogenic Differentiation and Calvarial Bone Defect Repair of Wharton’s Jelly Umbilical Cord Stem Cells
Authors: Wang Yantong , Zhang Simin , Yang Haoqing , Cao Yangyang , Yu Dianqin , Zhao Yingchu , cao yu 
Published: October 2021

Abstract
Background: To investigate the effect of miR‐196a-5p on the osteogenic differentiation and defected bone repair of Wharton’s jelly umbilical cord stem cells (WJCMSCs). Methods: miR‐196a-5p mimic or inhibitor was applied to overexpress or silence miR‐196a-5p expression in WJCMSCs. The alkaline phosphatase (ALP) activity, mineralization ability, and osteogenic markers expression were used to test WJCMSCs osteogenic potential in vitro. Calvarial bone defect model of rat was used to evaluate WJCMSCs bone regeneration ability in vivo. mRNA microarray was used to reveal the underling mechanisms that miR‐196a-5p regulated bone repair.Results: miR-196a-5p inhibition reduced the ALP activity, mineralization ability, and level of osteogenic markers OCN, DSPP, DMP1 and BSP, while miR-196a-5p overexpression enhanced the ALP activity, mineralization ability, and level of OCN, DSPP, DMP1 and BSP of WJCMSCs in vitro. Next, the micro-CT and histopathology results showed miR-196a-5p-overexpressed-WJCMSCs obviously promoted the new bone tissue regeneration and calvarial bone defect repair after MSCs transplanted for 12 weeks. Further, mRNA microarray of miR-196a-5p-overexpressed-WJCMSCs revealed totally 959 significantly differentially expressed genes (DEGs), among which 34 upregulated and 925 downregulated. Also, 241 miR-196a-5p targeted genes were predicted by using miRNA targeted websites and only 19 predicted genes were consistent with microarray results. On this basis, one significantly downregulated gene SERPINB2 was selected and revealed that SERPINB2 deletion obviously enhanced the ALP activity and mineralization ability of WJCMSCs in vitro.Conclusions: miR-196a-5p promoted the osteogenic differentiation potential and calvarial bone defect repair ability of WJCMSCs. And SERPINB2 acted as one key downstream gene to participate in the miR-196a-5p promoted MSCs osteogenic differentiation.
Safety and Efficacy of the Combination of BCc1 and Hep-S Nanochelating-Based Medicines in Hospitalized COVID-19 Adult Patients: A Randomized, Double-Blind, Placebo-Controlled Clinical Trial
Authors: Hafizi Maryam , Kalanaky Somayeh , Fakharzadeh Saideh , Fakharian Atefeh , Lookzadeh Somayeh , Mortaz Esmaeil , Mirenayat Maryam , Heshmatnia Jalal , Karam Mehrdad Bakhshayesh , Zamani Homa , Nadji Alireza , Toutkaboni Mihan Pourabdollah , Oraee-Yazdani Saeed , Akbari Mohammad Esmaeil , Jamaati Hamidreza , Nazaran Mohammad 
Published: October 2021

Abstract
Background: The mortality and morbidity of COVID‐19 disease as well as the lack of a proper medication has forced researchers and clinicians to employ urgent efficient technologies to overcome this current pandemic. In the severe forms of COVID-19, the patients develop a cytokine storm syndrome (CSS) where pro-inflammatory cytokines such as IL-6 and TNF-α play a key role in the development of this serious process. The efficiency of nanomedicines - as efficient immunomodulators - that are synthesized based on nanochelating technology have been proved in the previous studies. In the present study, the therapeutic effect of the combination of BCc1 and Hep-S nanomedicines on hospitalized COVID-19 patients was evaluated.Method: Laboratory-confirmed moderate COVID-19 patients at Masih Daneshvari Hospital were enrolled to participate in a randomized, double-blind, placebo-controlled study in two separate groups: combination of BCc1 and Hep-S (N=62) (treatment) or placebo (N=60) (placebo). The primary outcome of the study was evaluating the safety of the nanomedicines combination and its effect on the number of deceased patients, while the secondary outcome was decrease in inflammatory cytokines.Results: The evaluation of blood biochemical indices as well as clinical symptoms showed that adding the combination of BCc1 and Hep-S nanomedicines to the standard protocol of the treatment caused no adverse effects. The results analysis revealed that 28-day consumption of the nanomedicines led to a significant decrease in the mean level of IL-6 cytokine of the patients in the treatment group (p < 0.05). In addition, the patients in the treatment group had lower TNF-α levels compared to those in the control (p > 0.05) and they also showed less need for oxygen therapy. Finally, the number of the deceased patients in the treatment group was 30% lower than that of the control (p > 0.05).Conclusion: The combination of BCc1 and Hep-S, as safe nanomedicines, inhibits IL-6 as a highly important and well-known cytokine in COVID-19 pathophysiology, and presents a promising view for immunomodulation that can manage CSS and reduce mortality rate in COVID19 patients.Trial registration IRCTID, IRCT20170731035423N2. Registered 12 Jun 2020, http://www.irct.ir/ IRCT20170731035423N2.
Fluttering cord-like thrombus in the aortic arch: a case report
Authors: Kuroda Yuki , Marui Akira , Arai Yoshio , Nagasawa Atsushi , Tsumaru Shinichi , Arakaki Ryoko , Iida Jun , Wada Yuki , Soga Yoshiharu 
Published: October 2021

Abstract
BackgroundOptimal treatment for aortic thrombus remains to be determined, but surgical treatment is indicated when there is a risk for thromboembolism. Case PresentationA 47-year-old male presented with weakness in his left arm upon awakening. Contrast-enhanced computed tomography and transesophageal echocardiography revealed a mobile pedunculated object suggestive of a thrombus arising from the ascending aorta and extending to the left common carotid artery. It was removed under hypothermic circulatory arrest and direct cannulation of the left carotid artery to avoid carotid thromboembolism. Histopathological examination revealed that the object was a thrombus. The patient had an uneventful postoperative course and was discharged 9 days after surgery. ConclusionWhen a thrombus in the aortic arch extends to the neck arteries, direct cannulation of the neck arteries with selective cerebral perfusion via cervical incision is a useful technique.
Urosepsis After Percutaneous Nephrolithotomy (PCNL) - a New Prediction Rule and Scoring System
Authors: Yang Heng , Wang Zhang Qin , Liu Zhi Li , Hao Qiang Zhi , Wang Shen Jing , Wang Yuan , Li Le Yong , Tan Hui Ming , Qian Biao 
Published: October 2021

Abstract
Objective:Development and validation of a scoring system to predict the risk of urosepsis after percutaneous nephrolithotomy.Methods:The risk factors associated with urosepsis following PCNL(Percutaneous Nephrolithotomy) were identified by meta-analysis. Based on the degree of association, different scores were assigned to these risk factors. Finally Risk assessment scoring system for urosepsis after percutaneous nephrolithotomy (PCNL) was established and validated using ROC (Receiver Operating Characteristic) curve. Results:Based on the degree of association, Women, age (≥60yrs), diabetes mellitus, blood routine (White blood cells≥10×109/L), Urinalysis (White blood cells≥+), Urine culture (Positive), stone size(≥2cm), staghorn stone, hydronephrosis (moderate-severe) were assigned 3, 2, 3, 2, 2, 2, 2, 3, 2 points respectively with a total score of 21 points. The area under the ROC(Receiver Operating Characteristic) curve was 0.913, at the cut-off point of 8.5, the sensitivity and specificity were 90% and 89.4% respectively. Conclusions:The PuRass scoring system could be a useful tool in predicting the risk of urosepsis after PCNL(Percutaneous Nephrolithotomy). Clinician should pay attention to patients with a score above 8.5 during the perioperative period.
Risk Identification of Coal Spontaneous Fire Based on COWA Modified G1 Combination Weighting Cloud Model
Authors: Su Guorui , Jia Baoshan , Wang Peng , Zhang Ru , Shen Zhuo 
Published: October 2021

Abstract
To realize the scientific judgment of spontaneous fire risk in the coal mine, the spontaneous fire influence factors were analyzed from the three aspects of coal spontaneous combustion tendency, air leakage, and oxygen supply, heat storage and heat dissipation. And the basis for the evaluation of t spontaneous fire grade was constructed. Combination ordered weighted averaging (COWA) calculation was introduced to optimizes G1 subjective weighting, and a COWA modified G1 combined weighting cloud model was proposed to identify the spontaneous fire risk in the coal mine. Finally, the rationality of the model was verified with actual cases. The research results show that the spontaneous fire risk level in the Lingquan coal mine is relatively safe, which is consistent with the actual situation. And the spontaneous combustion tendency of coal is the leading factor affecting spontaneous fire risk.
Spatial-Temporal Distribution and Insecticide resistance Status of Aedes Aegypti in Ghana
Authors: Owusu-Asenso Christopher Mfum , Mingle Julius Abraham Addo , Weetman David , Afrane Yaw Asare 
Published: October 2021

Abstract
Background: Vector control is the main intervention to control arboviral diseases transmitted by Aedes mosquitoes because for most there are no effective vaccines or treatment. This vector control relies heavily on the use of insecticides, effectiveness of which may be impacted by resistance. In addition, rational insecticide application requires detailed knowledge of vector distribution, dynamics, resting, and feeding behaviours, which are poorly understood for Aedes vectors in Africa. This study investigated the spatio-temporal distribution and insecticide resistance status of Ae. aegypti from across ecological extremes of GhanaMethods: Immature mosquitoes were sampled from containers in and around human dwellings at each of seven study sites in urban, suburban, and rural areas of Ghana. Adult Aedes mosquitoes were sampled indoor and outdoor using Biogent sentinel-2 mosquito traps, human landing catches, and prokopack aspiration. Distributions of immatures and adult Aedes mosquitoes were determined indoors and outdoors during dry and rainy seasons at all sites. Phenotypic resistance status of Aedes mosquitoes to insecticides was determined using WHO bioassays. Host blood meal source was determined by PCR.Results: A total of 16,711 immature Aedes were sampled, with over 70% found in car tires. Significantly more breeding containers had Aedes immatures during the rainy season 70.95% (11,856) compared to the dry season 29.05% (4,855). A total of 1,895 adult Aedes mosquitos were collected, including Ae. aegypti (97.8%), Ae. africanus (2.1%) and Ae. Luteocephalus (0.1%). Indoor sampling of adult Aedes mosquitoes yielded a total of 381 (20.1%) and outdoor a total of 1,514 (79.9%) (z = -5.427; p = 0.0000) over the entire sampling period. Aedes aegypti populations were resistant to DDT at all study sites. Vectors showed suspected resistance to Bendiocarb (96-97%), Permethrin (90-96%) and Deltamethrin (91-96%) and were susceptible to the organophosphate malathion from all study sites.Blood meal analysis showed that the Aedes mosquitoes were mostly anthropophilic with HBI of 0.9 i.e. [(human = 90%), (human and dog = 5%), (dog and cow = 5%)].Conclusion: Aedes mosquitoes were found at high densities in all ecological zones of Ghana. Resistance to pyrethroids and carbamates may limit control efficacy and requires careful monitoring.
Development of a Prognostic Model Based on Differentially Expressed Immune Genes of Lung Adenocarcinoma
Authors: Zhai Yang , Li Lina , Wang Yuzhen , Li Jingjin , Li Xu , Liu Bin , Zhang Yili , Chen Qian 
Published: October 2021

Abstract
Due to the enormous heterogeneity and molecular complexity, the efficacy of existing lung adenocarcinoma risk prediction models were less than satisfactory. In this study, we downloaded the immune-related genes were from InnateDB, and the differentially expressed immune genes (DEIGs) were analyzed with edgeR and DESeq2 algorithm. In total, 1359 DEIGs were identified. Cytoscape was employed to build a mRNA-miRNA-lncRNA network which was consists of 8 lncRNAs, 7 miRNAs and 117 DEIGs. Functional enrichment analysis indicated that the 117 DEIGs were involved in immune and inflammatory responses and actively involved in a MAPK signaling pathway. A prognostic signature based on ten DEIGs including ASPH, CAV1, FKBP4, GRIK2, FURIN, SLC6A8, FSCN1, CKAP4, HAPLN2 and IL22RA2 which performed well was correlated with tumor burden, tumor stage and metastasis. The similar result was obtained in the validation dataset GSE72094 (p<0.0001). The prognostic index reflected infiltration by B cell, CD4+T cell, CD8+T cell and dendritic cell. We also found that ASPH and FSCN1 were over-expression in A549, H1299, PC-9 cells, and the positive expression of ASPH, FSCN1, MS4A1 and CD40LG had a correlation with the TNM stage, cellular differentiation and the lymphnode metastasis(p<0.05). High levels of ASPH and FSCN1, low levels of MS4A1 and CD40LG expression are all associated with poor overall survival in LUAD (p<0.05). In conclusion, our results identified DEIGs of clinical significance, verified the effect of the DEIGs-based, personalized prediction model for lung adenocarcinoma prognosis. Together, our results revealed that ASPH and FSCN1 could be a prognostic marker for lung adenocarcinoma.