bluuebunny/crossref_metadata_2025_split huggingface dataset:
BioF is a novel B2 metallo‐β‐lactamase from Pseudomonas sp. isolated from an on‐farm biopurification system
Authors: Cafiero Juan Hilario , Martini María Carla , Lozano Mauricio Javier , Vacca Carolina , Lagares Antonio , Tomatis Pablo Emiliano , Del Papa María Florencia 
Published: November 2021

SummaryAntimicrobial resistance represents a major global health concern and environmental bacteria are considered a source of resistance genes. Carbapenems are often used as the last antibiotic option to treat multidrug‐resistant bacteria. Metallo‐β‐lactamases (MBLs) are able to render resistance to almost all β‐lactam antibiotics, including carbapenems. Unfortunately, there are no inhibitors against MBLs for clinical use. Subclass B2 MBLs are the only enzymes working as strict carbapenemases, under‐represented, encoded in chromosome genes and only functional as mono‐zinc enzymes. Despite current efforts in MBLs inhibitor development, B2 carbapenemase activity is especially difficult to suppress, even in vitro. In this study we characterized BioF, a novel subclass B2 MBL identified in a new environmental Pseudomonas sp. strain isolated from an on‐farm biopurification system (BPS). Although blaBioF is most likely a chromosomal gene, it is found in a genomic island and may represent a step previous to the horizontal transmission of B2 genes. The new B2 MBL is active as a mono‐zinc enzyme and is a potent carbapenemase with incipient activity against some cephalosporins. BioF activity is not affected by excess zinc and is only inhibited at high metal chelator concentrations. The discovery and characterization of B2 MBL BioF as a potent carbapenemase in a BPS bacterial isolate emphasizes the importance of exploring antibiotic resistances existing in the environmental microbiota under the influence of human activities before they could emerge clinically.
Getting high or getting low? the external effects of coffeeshops on house prices
Authors: Langen Mike , Aydin Erdal , Eichholtz Piet , Kok Nils 
Published: November 2021

AbstractCannabis legalization is a hotly contested policy topic. While beneficial to some, cannabis dispensaries may create negative externalities for others. This paper studies the external effects of coffeeshops—Dutch cannabis sales facilities—on house prices. We employ a difference‐in‐difference framework around a change in regulation, leading to exogenous coffeeshop closings. We find that closings have a negative effect on house prices. Compared to homes nearby remaining coffeeshops, homes nearby closing coffeeshops decrease on average 1.6–8.5% in value. The findings are robust to a battery of tests and unaffected by the subsequent use of coffeeshop locations.
Successful procedure with additional omentopexy to suture closure of gallbladder stump in laparoscopic subtotal cholecystectomy
Authors: Kato Hirotaka , Kinoshita Hiroyuki , Kawaguchi Masanori , Yamazaki Hirofumi , Sakata Yoshifumi 
Published: November 2021

AbstractLaparoscopic subtotal cholecystectomy, a bailout surgery for cholecystitis, can result in postoperative bile leakage, so surgical ingenuity is required. An 88‐year‐old woman had pain at the right hypochondrium. Abdominal computed tomography showed swelling of the gallbladder and thickness of the gallbladder wall, leading to diagnosis of mild acute cholecystitis. Percutaneous transhepatic gallbladder drainage was performed to alleviate cholecystitis because the patient was taking antiplatelet medicine. Laparoscopic cholecystectomy was then performed within 72 hours from the onset. The gallbladder was operatively found to be strongly fibrotic, so the procedure was switched to laparoscopic subtotal cystectomy, dissecting the gallbladder at the infundibulum‐cystic duct level. The gallbladder stump was closed with barbed suture and omentopexy was added due to fragility. There was no significant postoperative bile leakage. Additional omentopexy to stump closure in laparoscopic subtotal cholecystectomy was thought to be useful in prevention of postoperative bile leakage.
Parenting and the development of children’s gratitude
Authors: Hussong Andrea M. , Coffman Jennifer L. , Halberstadt Amy G. 
Published: November 2021

AbstractFostering gratitude is often among the socialization goals parents hold for their children. In this article, we explore work that portrays gratitude as a complex socioemotional process that occurs during a moment in time and becomes more frequent, integrated, and rich with development. Researchers have identified at least four parent socialization practices that may foster children’s gratitude: modeling, daily scaffolding, niche selection, and conversations with children about gratitude moments and missed opportunities for gratitude. Parent training tools based on this work have yielded modest results. More work is needed to understand further the ways parenting and children’s gratitude are culturally embedded, place the work on gratitude socialization within the larger task of value socialization faced by parents, and explore gratitude as an ontogenetic tool for making meaning of the world around us.
Association between diabetes mellitus and chronic rhinosinusitis with nasal polyps: A population‐based cross‐sectional study
Authors: Nam Jae‐Sung , Roh Yun‐Ho , Kim Jungghi , Chang Suk‐Won , Ha Jong‐Gyun , Park Jeong‐Jin , Fahad Wasan Almazouq , Yoon Joo‐Heon , Kim Chang‐Hoon , Cho Hyung‐Ju 
Published: November 2021

AbstractObjectiveTo investigate the association between physician‐diagnosed diabetes mellitus (DM) and chronic rhinosinusitis (CRS) phenotypes in a national population‐based study.Study DesignRetrospective cross‐sectional study.SettingPopulation‐based survey data were collected by the Korean National Health and Nutrition Survey between January 2008 and December 2012.Participants and MethodsA total of 34 670 participants aged over 19 years were enrolled in the Korea National Health and Nutrition Examination Surveys from 2008 to 2012. The relationship of CRS prevalence, with and without nasal polyps, with physician‐diagnosed DM and non‐DM were assessed. Differences in sinonasal symptoms between patients with and without DM were analysed in this cross‐sectional study.ResultsA significant association was observed between DM and CRS with nasal polyps after adjustment for multiple variables. No substantial association was observed between DM and CRS without nasal polyps. Among patients with CRS, olfactory dysfunction for >3 months was significantly more frequent in the DM group than in the non‐DM group.ConclusionWe demonstrated significant associations between DM and CRS with nasal polyps and olfactory dysfunction among patients with CRS in a large national clinical cohort study. The direct mechanism of the association between DM and CRS with nasal polyps should be further investigated to clarify the pathogenesis of CRS with nasal polyps.
Rectal washout during abdominoperineal resection for rectal cancer has no impact on the oncological outcome
Authors: Svensson Neufert Rebecca , Jörgren Fredrik , Buchwald Pamela 
Published: November 2021

AbstractAimIntraoperative rectal washout is performed to eliminate exfoliated intraluminal cancer cells and thereby decrease the risk of local recurrence. Rectal washout in abdominoperineal resection has not been studied. The aim of this study was to assess the oncological outcome after rectal washout in abdominoperineal resection for rectal cancer and to find evidence as to whether rectal washout should be performed or not.MethodData for all patients registered in the Swedish Colorectal Cancer Registry who underwent elective surgery with abdominoperineal resection for rectal cancer (TNM Stages I–III) between 2007 and 2013 were analysed using multivariable analysis.ResultsNo significant differences were shown between the rectal washout group and the no rectal washout group for local recurrence [10/265 (3.8%) vs. 87/2160 (4.0%), p = 0.84], distant metastasis [51/265 (19.2%) vs. 476/2160 (22.0%), p = 0.29] or overall recurrence [53/265 (20.0%) vs. 505/2160 (23.4%), p = 0.21]. In multivariable analysis, rectal washout did not significantly affect the oncological outcome in terms of local recurrence, distant metastasis, overall recurrence or 5‐year overall or relative survival.ConclusionOur results do not support routine rectal washout during abdominoperineal resection in order to improve the oncological outcome.
Osteoblasts impair cholesterol synthesis in chondrocytes via Notch1 signalling
Authors: Yang Yueyi , Zhang Demao , Guo Daimo , Li Jiachi , Xu Siqun , Wei Jieya , Xie Jing , Zhou Xuedong 
Published: November 2021

AbstractObjectivesPrevious reports have proposed the importance of signalling and material exchange between cartilage and subchondral bone. However, the specific experimental evidence is still insufficient to support the effect of this interdependent relationship on mutual cell behaviours. In this study, we aimed to investigate cellular lipid metabolism in chondrocytes induced by osteoblasts.MethodsOsteoblast‐induced chondrocytes were established in a Transwell chamber. A cholesterol detection kit was used to detect cholesterol contents. RNA sequencing and qPCR were performed to assess changes in mRNA expression. Western blot analysis was performed to detect protein expression. Immunofluorescence staining was conducted to show the cellular distribution of proteins.ResultsCholesterol levels were significantly decreased in chondrocytes induced by osteoblasts. Osteoblasts reduced cholesterol synthesis in chondrocytes by reducing the expression of a series of synthetases, including Fdft1, Sqle, Lss, Cyp51, Msmo1, Nsdhl, Sc5d, Dhcr24 and Dhcr7. This modulatory process involves Notch1 signalling. The expression of ncstn and hey1, an activator and a specific downstream target of Notch signalling, respectively, were decreased in chondrocytes induced by osteoblasts.ConclusionsFor the first time, we elucidated that communication with osteoblasts reduces cholesterol synthesis in chondrocytes through Notch1 signalling. This result may provide a better understanding of the effect of subchondral bone signalling on chondrocytes.
Manchester Intermittent versus Daily Diet App Study (MIDDAS): A pilot randomized controlled trial in patients with type 2 diabetes
Authors: McDiarmid Sarah , Harvie Michelle , Johnson Rhona , Vyas Avni , Aglan Azza , Moran Jacqui , Ruane Helen , Hulme Amanda , Sellers Katharine , Issa Basil G. 
Published: November 2021

AbstractAimsTo test the feasibility and potential efficacy of remotely supported intermittent low‐energy diets (ILEDs) and continuous low‐energy diets (CLEDs) in people with type 2 diabetes (T2D) and the feasibility of a randomized controlled trial comparing the two approaches.Materials and methodsSeventy‐nine adults with overweight/obesity and T2D (≤8 years duration) were randomized 1:1 to CLED (8 weeks/56 days of daily Optifast 820 kcal (3430 kJ) diet) or isoenergetic ILED (2 days of Optifast and 5 days of a Mediterranean diet/week for 28 weeks). Weight maintenance/continued weight loss was undertaken for the remainder of the 52 weeks. Both groups received frequent telephone or the Oviva app support. Feasibility outcomes included study uptake, retention, app usage, dietary adherence, weight loss and change in glycated haemoglobin (HbA1c) at 52 weeks.ResultsWe enrolled 39 ILED and 40 CLED participants and 27 (69%) ILED and 30 CLED (75%) attended the 52‐week follow‐up. Eighty‐nine per cent (70 of 79) started using the app and 86% (44 of 51) still used the app at 52 weeks. Intention‐to‐treat analysis at 52 weeks showed percentage weight loss was mean (95% confidence interval) −5.4% (−7.6, −3.1%) for ILED and −6.0% (−7.9, −4.0%) for CLED. HbA1c <48 mmol/mol was achieved in 42% of both groups. Mean (95% confidence interval) changes in the T2D medication effect score were 0.0008 (−0.3, 0.3) for ILED and −0.5 (−0.8, −0.3) for CLED.ConclusionThe study shows the feasibility and potential efficacy of remotely delivered ILED and CLED programmes for weight loss and HbA1c reduction, and the feasibility of a randomized controlled trial comparing the two approaches.
Youth and family members make meaningful contributions to a randomized‐controlled trial: YouthCan IMPACT
Authors: Henderson Joanna , Courey Lynn , Relihan Jacqueline , Darnay Karleigh , Szatmari Peter , Cleverley Kristin , Cheung Amy , Hawke Lisa D. 
Published: November 2021

AbstractBackgroundThere are growing calls to engage service users in research about issues relevant to them. Youth and family members can make meaningful contributions to research projects, improving quality and relevance. However, more information is needed on the contributions that youth and family members can make to various study designs.ObjectiveThis paper describes the contributions that youth and family members have made to a multi‐site pragmatic randomized‐controlled trial, YouthCan IMPACT, and the way project‐based engagement learnings accelerated change at the institutional level and beyond.ResultsYouth and family members were full members of the project team, including the project's core governance and working groups. They contributed to project leadership, as funding co‐applicants and as equal members of the governance team. They were also engaged in study design. Youth defined the primary outcome measure and contributed to decisions on all secondary measures. The service pathway was co‐designed with youth and family members; for example, they guided the inclusion of peer support and a family member intervention as core service components. Study implementation contributions included ensuring a youth‐ and family‐friendly research process and training research staff on working with youth and family members. Knowledge translation activities have included youth and family members as co‐presenters and manuscript co‐authors. The learnings from this trial have been leveraged to expand youth and family engagement at the institution and beyond.ConclusionsYouth and family members make substantial contributions to complex research projects, including randomized‐controlled trials, thereby improving project design, study implementation, associated interventions, and knowledge translation.
Genuine and non‐genuine smiles in individuals meeting criteria for a clinical high‐risk syndrome
Authors: Ricard Jordyn R. , Gupta Tina , Vargas Teresa , Haase Claudia M. , Mittal Vijay A. 
Published: November 2021

AbstractAimPsychosis is characterized by both alterations in emotional functioning and environmental stressors including bullying victimization. Recent evidence suggests that some alterations in emotional functioning (e.g., blunted positive facial expressions) are already present in the psychosis risk period. Yet, some clinically relevant facial emotions have not been investigated such as genuine smiles (thought to reflect genuine positive emotions) and non‐genuine smiles (thought to fake positive or mask negative emotions) in individuals meeting criteria for a clinical high‐risk (CHR) syndrome. Further, despite a compelling conceptual basis to suggest a link between affective expression and exposure to environmental stress, to date, no investigations have sought to examine this association. Here, we aim to assess differences between a sample of CHR (N = 65) and control (N = 67) individuals in genuine and non‐genuine smiles and associations with bullying victimization.MethodsSmiles (i.e., genuine; non‐genuine) were objectively coded on a second‐by‐second basis using the Facial Action Coding System during a digitally recorded clinical interview segment. Bullying victimization was measured via parent report.ResultsFindings revealed that the CHR group (1) showed blunted genuine (but not non‐genuine) smiles compared to controls. Moreover, (2) bullying victimization was related to blunted genuine smiles, but not non‐genuine smiles.ConclusionThese findings expand our understanding of emotional alterations in this group with implications for diagnosis (highlighting blunted genuine smiles as a specific marker) and etiology (underscoring the role of bullying victimization in the etiology of emotional dysfunction).
Course of insight, depression and suicidality in inpatients with first‐episode schizophrenia who received group psychoeducation
Authors: Sato Kojiro , So Ryuhei , Yoshimura Bunta , Kitagawa Kohei 
Published: November 2021

AbstractAimWe aimed to investigate whether gaining insight through psychoeducation for first‐episode schizophrenia is associated with increased suicidality.MethodsWe conducted a secondary analysis of a prospective cohort study that included inpatients with first‐episode schizophrenia who attended a group psychoeducation program during their admission. The group psychoeducation program consisted of four weekly sessions provided by a multidisciplinary team. The primary outcome was the correlation between changes in insight and suicidality. We also examined whether change in insight was associated with changes in hopelessness and depression. We measured insight using the Birchwood Insight Scale. Suicidality, hopelessness and depression were measured using the Calgary Depression Rating Scale for Schizophrenia.ResultsA total of 125 people participated in the educational program. The Spearman's correlation coefficient between changes in insight and suicidality was −0.14 (95% confidence interval, −0.31 to 0.04; p = .12). Similarly, gain in insight did not significantly correlate with change in depression (0.01, 95% confidence interval, −0.17 to 0.18; p = .93) and change in hopelessness (0.01, 95% confidence interval, −0.16 to 0.19; p = .88).ConclusionsWe observed almost no association between gaining insight and suicidality after a group psychoeducation program in inpatients with first‐episode schizophrenia.
Pathogenesis of solar urticaria: Classic perspectives and emerging concepts
Authors: McSweeney Sheila Mary , Sarkany Robert , Fassihi Hiva , Tziotzios Christos , McGrath John Alexander 
Published: November 2021

AbstractSolar urticaria is a rare, immunologically mediated photodermatosis in which activation of cutaneous mast cells is triggered by specific wavelengths of solar electromagnetic radiation. This manifests clinically as the rapid development of cutaneous itch, erythema and wheal formation after several minutes of sun exposure. Disease mechanisms in solar urticaria remain incompletely elucidated and there have been few recent investigations of its pathobiology. Historic passive transfer experiments performed during the twentieth century provide support for a ‘photoallergy’ model of disease pathogenesis, wherein molecular alteration of a putative chromophore by solar electromagnetic radiation produces mast cell activation via an IgE‐dependent mechanism. However, this model does not account for several observations made during passive transfer experiments nor does it explain a range of subsequent clinical and photobiological observations made in solar urticaria patients. Furthermore, increased understanding of the molecular dynamics underpinning cutaneous mast cell responses highlights the need to reformulate our understanding of solar urticaria pathogenesis in the context of this contemporary scientific landscape. In this review, we discuss the current understanding of solar urticaria pathogenesis and, by incorporating recent scientific and clinical observations, develop new hypotheses to drive future investigation into this intriguing disorder.
Coupling plant litter quantity to a novel metric for litter quality explains C storage changes in a thawing permafrost peatland
Authors: Hough Moira , McCabe Samantha , Vining S. Rose , Pickering Pedersen Emily , Wilson Rachel M. , Lawrence Ryan , Chang Kuang‐Yu , Bohrer Gil ,   The IsoGenie Coordinators, Riley William J. , Crill Patrick M. , Varner Ruth K. , Blazewicz Steven J. , Dorrepaal Ellen , Tfaily Malak M. , Saleska Scott R. , Rich Virginia I. 
Published: November 2021

AbstractPermafrost thaw is a major potential feedback source to climate change as it can drive the increased release of greenhouse gases carbon dioxide (CO2) and methane (CH4). This carbon release from the decomposition of thawing soil organic material can be mitigated by increased net primary productivity (NPP) caused by warming, increasing atmospheric CO2, and plant community transition. However, the net effect on C storage also depends on how these plant community changes alter plant litter quantity, quality, and decomposition rates. Predicting decomposition rates based on litter quality remains challenging, but a promising new way forward is to incorporate measures of the energetic favorability to soil microbes of plant biomass decomposition. We asked how the variation in one such measure, the nominal oxidation state of carbon (NOSC), interacts with changing quantities of plant material inputs to influence the net C balance of a thawing permafrost peatland. We found: (1) Plant productivity (NPP) increased post‐thaw, but instead of contributing to increased standing biomass, it increased plant biomass turnover via increased litter inputs to soil; (2) Plant litter thermodynamic favorability (NOSC) and decomposition rate both increased post‐thaw, despite limited changes in bulk C:N ratios; (3) these increases caused the higher NPP to cycle more rapidly through both plants and soil, contributing to higher CO2 and CH4 fluxes from decomposition. Thus, the increased C‐storage expected from higher productivity was limited and the high global warming potential of CH4 contributed a net positive warming effect. Although post‐thaw peatlands are currently C sinks due to high NPP offsetting high CO2 release, this status is very sensitive to the plant community's litter input rate and quality. Integration of novel bioavailability metrics based on litter chemistry, including NOSC, into studies of ecosystem dynamics, is needed to improve the understanding of controls on arctic C stocks under continued ecosystem transition.
Variable Selection for Interval‐censored Failure Time Data
Authors: Du Mingyue , Sun Jianguo 
Published: November 2021

SummaryVariable selection for interval‐censored failure time data has recently attracted a great deal of attention along with the analysis of interval‐censored data in both method developments and practical applications. Interval‐censored data are a general type of time‐to‐event or failure time data where the failure time of interest is known or observed only to lie in an interval instead of being observed exactly. They often occur in different forms and in many fields, including demographical studies, epidemiological studies, medical or public health researches and social sciences. This paper will discuss and review the existing methods for variable selection based on interval‐censored data, a relatively new but important topic, along with some directions or issues that need more research.