bluuebunny/crossref_metadata_2025_split huggingface dataset:
Serotoninergic Receptor Ligands Improve Tamoxifen Effectiveness on Breast Cancer Cells
Authors: Ambrosio Maria Rosaria , Magli Elisa , Caliendo Giuseppe , Sparaco Rosa , Massarelli Paola , D'Esposito Vittoria , Migliaccio Teresa , Mosca Giusy , Fiorino Ferdinando , Formisano Pietro 
Published: February 2021

Abstract
Background: The tightly regulated pattern of serotonin (or 5-Hydroxytryptamine, 5-HT) in mammary gland becomes dysregulated in cancer, also contributing to proliferation, metastasis and angiogenesis. Antagonists of serotonin receptors or inhibitors of serotonin reuptake/synthesis may be co-prescribed with Tamoxifen in estrogen receptor-positive (ER+) tumors, and may modulate its efficacy. Thus, the discovery of novel compounds targeting serotonin signaling may contribute to tailor new therapeutic strategies usable in combination with endocrine therapies. We have previously synthesized serotoninergic receptor ligands (SER) with high affinity and selectivity towards 5-HT2A and 5-HT2C receptors, the main mediators of mitogenic effect of serotonin in breast cancer (BC). Here, we investigated the effect of 10 SER on viability of MCF7, SKBR3 and MDA-MB231 BC cells and focused on their potential ability to affect Tamoxifen responsiveness in ER+ cells. Methods: Cell viability has been assessed by sulforhodamine B assay. Cell cycle has been analyzed by flow cytometry. Gene expression of 5-HT receptors and Connective Tissue Growth Factor (CTGF) has been checked by RT-PCR; CTGF mRNA levels have been measured by qPCR. All data were statistically analyzed using GraphPad Prism 7. Results: We found that SER were more effective on MCF7 ER+ cells (IC50 range 10.2µM - 99.2µM) compared to SKBR3 (IC50 range 43.3µM - 260µM) and MDA-MB231 BC cells (IC50 range 91.3µM - 306µM). Next, we provided evidence that two ligands, SER79 and SER68, improved the effectiveness of Tamoxifen treatment in MCF7 BC cells and modulated the expression of CTGF. Moreover, in a cell model of Tamoxifen-resistance, SER79 and SER68 restored drug responsiveness and reduced CTGF levels. Conclusions: These results identified new compounds potentially usable in combination with Tamoxifen to improve its effectiveness on ER+ BC patients.
High serum creatinine in early life of preterm infants predicts the severe retinopathy of prematurity: a retrospective single-centre study
Authors: Bae Seong Phil , Kim Sung Shin , Han Won-Ho , Kim Ho , Koh Ji Won , Song Jun Hwan , Park Soo Yeon 
Published: February 2021

Abstract
BACKGROUND

Hypoxia and anemia are among the risk factors for retinopathy of prematurity (ROP). The kidneys are important organs that sense oxygen levels and regulate red blood cell synthesis via erythropoietin production. We investigated the contribution of abnormal renal function (reflected by serum creatinine [SCr] levels) to severe ROP in very low birth weight (VLBW) infants.
METHODS

In the present study, we enrolled 242 VLBW infants of gestational age (GA) ranging between 25 and 32 weeks who were admitted at Soonchunhyang Cheonan University Hospital between Nov 2014 and Dec 2019. The cut-off value for normal SCr for each GA group based was defined as 95th percentile of SCr based on a reference chart developed in a previous study. Risk factors for ROP requiring treatment were analyzed using logistic regression.
RESULTS

Of the 242 infants, 63 (26%) were high SCr group and 30 (12.4%) infants had ROP requiring treatment. GA (odds ratio 0.38, 95% confidence interval 0.23–0.61) and high SCr group (4.68 [1.10–19.90]) were independent factors for ROP requiring treatment.
CONCLUSIONS

In VLBW infants, high SCr within the first 4 weeks after birth is one of the risk factors for ROP requiring treatment.
Forest quality mitigates extinction risk in humid tropical vertebrates
Authors: Pillay Rajeev , Watson James , Hansen Andrew , Aragon-Osejo Jose , Armenteras Dolors , Atkinson Scott , Burns Patrick , Ervin Jamison , Goetz Scott , Gonzalez-del-Pliego Pamela , Jantz Patrick , Supples Christina , Virnig Anne Lucy , Williams Brooke , Venter Oscar 
Published: February 2021

Abstract
Reducing deforestation underpins efforts to conserve global biodiversity. However, this focus on retaining forest cover overlooks the multitude of anthropogenic pressures that can degrade forest quality in ways that may imperil biodiversity. Here we use the latest remotely-sensed measures of forest structural condition and associated human pressures across the global humid tropics to provide the first estimates of the importance of forest quality, relative to forest cover, in mitigating extinction risk for rainforest vertebrates worldwide. We found tropical rainforests of intact structural condition and minimal human pressures played an outsized role in reducing the odds of species being threatened or having a declining population. Further, the effects of forest quality in mitigating extinction risk were stronger when small amounts of high quality forest remained within species geographic ranges, as opposed to when large extents were forested but of low quality. Our research underscores a critical need to focus global environmental policy and conservation strategies toward the targeted protection of the last remaining undisturbed forest landscapes, in concert with strategies aimed at preserving, restoring and reconnecting remnant forest fragments across the hyperdiverse humid tropics.
Antireflux endoscopic injection therapy in adult patients via modified hydrodistention implantation techniques adopted for the dilated ureteral orifice: a retrospective single-center study
Authors: Maruyama Tetsuji , Mizuno Kentaro , Nishio Hidenori , Kato Taiki , Hamakawa Takashi , Ikegami Yosuke , Yasui Takahiro , Hayashi Yutaro 
Published: February 2021

Abstract
Background
To investigate the efficacy and safety of endoscopic injection therapy for vesicoureteral reflux in adult patients with dilated ureteral orifice via modified hydrodistension implantation techniques.
Methods
We retrospectively reviewed medical records including operational procedure and clinical course of all consecutive patients over 12 years old with a history of injection therapy. Endoscopic injection of dextranomer /hyaluronic acid copolymer was performed under hydrodistension implantation technique with some modifications in order to inject through dilated ureteral orifice align with the intramural ureter. Technical selections were done according to hydrodistension grade of the ureteral orifice. Voiding cystourethrography was evaluated at 3 months postoperatively. Hydronephrosis was evaluated using ultrasonography preoperatively until 6 months postoperatively.
Results
From 2016 to 2019, 12 patients (all female, 16 ureteral units; median age 32 [range: 15 to 61] years) underwent endoscopic injection therapy at one of our institutions. We have identified grade II vesicoureteral reflux in 5 ureters, grade III in 8, and grade IV in 3 ureters. Grade 3 ureteral-orifice dilation were presented in 12 ureters (75%), grade 2 in 3 and grade 1 in ureter in the present cases. Postoperatively, vesicoureteral reflux was diminished to grade 0 in 12 ureteral units (75%), decreased to grade I in 3 (9%), and remained grade III in 1 (6%). Three patients reported dull flank pain for several days postoperatively and there was 1 case of acute pyelonephritis. Temporary hydronephrosis was confirmed in 3 ureteral units (19%) at 1 month postoperatively.
Conclusions
According to hydrodistension grade of the ureteral orifice, endoscopic injection therapy via modified hydrodistension implantation technique is an effective and safe treatment for vesicoureteral reflux in adult patients with dilated ureteral orifice.
Direct mapping of Peptide-to-Spectra-Matches to genome information facilitates qualifying proteomics information
Authors: Anders John , Petruschke Hannes , Jehmlich Nico , Haange Sven-Bastiaan , Bergen Martin von , Stadler Peter F 
Published: February 2021

Abstract
Background: Small Proteins have received increasing attention in recent years. They have in particular been implicated as signals contributing to the coordination of bacterial communities. In genome annotations they are often missing or hidden among large numbers of hypothetical proteins because genome annotation pipelines often exclude short open reading frames or over-predict hypothetical proteins based on simple models. The validation of novel proteins, and in particular of small proteins (sProteins), therefore requires additional evidence. Proteogenomics is considered the gold standard for this purpose. It extends beyond established annotations and includes all possible open reading frames (ORFs) as potential sources of peptides, thus allowing the discovery of novel, unannotated proteins. Typically this results in large numbers of putative novel small proteins fraught with large fractions of false-positive predictions.
Results: We observe that number and quality of the Peptide-to-Spectra-Matches (PSMs) that map to a candidate ORF can be highly informative for the purpose of distinguishing proteins from spurious ORF annotations. We report here on a workflow that aggregates PSM quality information and local context into simple descriptors and reliably separates likely proteins from the large pool of false-positive, i.e., most likely untranslated ORFs. We investigated the artificial gut microbiome model SIHUMIx, comprising eight different species, for which we validate 5114 proteins that previously have been annotated only as hypothetical ORFs. In addition, we identified 37 non-annotated protein candidates for which we found evidence in proteomic and transcriptomic level. Half (19) of these candidates have close functional homologs in other species. Another 12 candidates have homologs designated as hypothetical proteins in other species. The remaining six candidates are short (< 100 AA) and are most likely bona fide novel proteins.
Conclusions: The aggregation of PSM quality information for predicted ORFs provides a robust and efficient method to identify novel proteins in proteomics data. The workflow is in particular also capable of identifying small proteins and frameshift variants. Since PSMs are explicitly mapped to genomic locations, it furthermore facilitates the integration with transcriptomics data and other source of genome-level information.
Are Hormonal Agents Better Than Chemo İn Metastati̇c Castrati̇on Resistant Prostate Cancer?
Authors: Yıldırım Serkan , ERDOĞAN Atike Pınar , Yılmaz Cengiz , Ekinci Ferhat , Bulut Gülcan , Özveren Ahmet 
Published: February 2021

Abstract
İNTRODUCTİONIn this study, we aim to determine which treatment is more appropriate in castration-resistant chemotherapy-naive patients. Therefore, docetaxel and agents active in the androgen pathway (abiraterone and enzalutamide) were compared retrospectively in patients progressing on ADT.MATERİAL METHODThe study was designed as a retrospective and multi-center study. Patients from 5 centers in Turkey were included in the study. The primary endpoint of the study was ovreall survival and the secondary endpoint was progression-free survival.RESULTSMedian overall survival of the chemotherapy group was 18.66 months, it was 16.26 months in the hormonal treatment group. There was no statistically significant difference between the groups (p = 0.311). Median progression-free survival of the chemotherapy group was 5.6 months, while it was 9 months in the hormonal therapy group. There was statistically significant difference between the groups (p = 0.024).CONCLUSİONThere was statistical difference in progression-free survival in favor of hormonal therapies in our study. The difference did not reflect on overall survival and there was no difference between hormonal therapies and docetaxel. Heterogeneity in the selection of patients is considered to lead to this result; however, larger randomized controlled studies are needed to determine the most appropriate treatment in these patients.
Adenocarcinoma with mixed subtypes in the early and advanced gastric cancer.
Authors: Zhao Xixian , Li Yizhang , Yang Zhenwei , Zhang Hailin , Wang Hongling , Lin Jun , Zhao Qiu , Liu Jing 
Published: February 2021

Abstract
Background

Based on the WHO classification, adenocarcinoma with mixed subtypes (AM) is a histological classification. We aimed to compare the prognosis among AM, classic adenocarcinoma (CA), mucinous adenocarcinoma (MAC), and signet-ring cell carcinoma (SRCC) in early and advanced gastric cancer (EGC, AGC), respectively.
Methods

We compared the clinicopathologic features and prognosis between AM and other histologic subtypes of CA, SRCC and MAC in ECG and ACG, respectively. A nomogram was established to predict the cancer-specific survival (CSS) of gastric cancer (GC) patients with AM. C-index, calibration curves, Receiver Operating Characteristic (ROC) and Decision Curve Analysis (DCA) curves were applied to examine the accuracy and clinical benefits.
Results

In the prognosis among these four histological subtypes in EGC patients, there are no differences. For AGC patients, AM had a significantly poorer prognosis compared with CA and MAC (P = 0.003, 0.029), but similar prognosis to SRCC. A nomogram based on race, T stage, N stage, M stage and surgical modalities were proposed to predict 1- and 3- year CSS for GC patients with AM (C-index: training cohort: 0.804, validation cohort: 0.748. 1-, 3-year CSS AUC: training cohort: 0.871, 0.914, validation cohort: 0.810, 0.798). 1- and 3-year CSS DCA curves showed good net benefits.
Conclusions

EGC patients with AM had similar survival to those with CA, MAC and SRCC. AM was an independent predictor of poor prognosis in AGC. A nomogram for predicting the prognosis of GC patients with AM was proposed to quantitatively assess the long-term survival.
Give Me a Pain that I am used to: Distinct Habituation Patterns to Painful &amp; Non-painful Stimulation
Authors: Paul Katharina , Tik Martin , Hahn Andreas , Sladky Ronald , Geissberger Nicole , Seidel Eva-Maria , Kranz Georg S. , Pfabigan Daniela M. , Kraus Christoph , Lanzenberger Rupert , Lamm Claus , Windischberger Christian 
Published: February 2021

Abstract
Pain habituation is associated with a decrease of activation in brain areas related to pain perception. However, little is known about the specificity of these decreases to pain, as habituation has also been described for other responses like spinal reflexes and other sensory responses. Thus, it might be hypothesized that previously reported reductions in activation are not specifically related to pain habituation. For this reason, we performed a 3T fMRI study using either painful or non-painful electrical stimulation via an electrode attached to the back of the left hand. Contrasting painful vs. non-painful stimulation revealed significant activation clusters in regions well-known to be related to pain processing, such as bilateral anterior and posterior insula, primary/secondary sensory cortices (S1/S2) and anterior midcingulate cortex (aMCC). Importantly, our results show distinct habituation patterns for painful (in aMCC) and non-painful (contralateral claustrum) stimulation, while similar habituation for both types of stimulation was identified in bilateral inferior frontal gyrus (IFG) and contralateral S2. Our findings thus distinguish a general habituation in somatosensory processing (S2) and reduced attention (IFG) from specific pain and non-pain related habituation effects where pain-specific habituation effects within the aMCC highlight a change in affective pain perception.
The Fractional Nonlinear PT Dimer
Authors: Molina Mario I. 
Published: February 2021

Abstract
We examine a fractional Discrete Nonlinear Schrodinger dimer, where the usual first-order derivative of the time evolution is replaced by a non integer-order derivative. The dimer is nonlinear (Kerr) and PT-symmetric, and we examine the exchange dynamics between both sites. By means of the Laplace transformation technique, the linear PT dimer is solved in closed form in terms of Mittag-Leffler functions, while for the nonlinear regime, we resort to numerical computations using the direct explicit Grunwald algorithm. In general, the main effect of the fractional derivative is the onset of a monotonically decreasing time envelope for the amplitude of the oscillatory exchange. In the presence of PT symmetry, the dynamics shows damped oscillations for small gain/loss in both sites, while at higher gain/loss parameter values, the amplitudes of both sites grows unbounded. In the presence of nonlinearity, selftrapping is still possible although the trapped fraction decreases as the nonlinearity is increased past threshold, in marked contrast with the standard case.
The untrained response of pet dogs to human epileptic seizures
Authors: Powell Neil Anthony , Ruffell Alastair , Arnott Gareth 
Published: February 2021

Abstract

Epilepsy is a disorder of the brain and a seriously debilitating condition, which has been associated with injury, social stigmatisation and in some cases, sudden unexpected and premature death. A sense of profound isolation is felt by many individuals with epilepsy, and this community has expressed an urgent desire for an early warning system to allow them time to prepare for seizure onset. Surveys of dog owners with epilepsy have previously reported that some dogs can predict the onset of a seizure. Therefore, the current study investigated the hypothesis that if pet dogs were exposed to seizure-related odours which apparently emanated from their owners, they would respond by demonstrating attention seeking behaviours. This provides the first empirical test of this phenomenon in dogs that have not previously been trained for seizure alerting. This study, therefore, explores the propensity of pet dogs to anticipate and respond to human epileptic seizure onset in a controlled experimental investigation. Using a repeated measures design of experiment, recordings were made of the reactions of 19 untrained pet dogs to odours from sweat samples provided by three people with epilepsy and two people without epilepsy (controls). The sweat samples were from pre-ictal, ictal and post-ictal phases. Odours from the harvested sweat samples were randomly delivered to individual dogs in a test area, using two bespoke pieces of apparatus called Remote Odour Delivery Mechanisms, (RODMs). Behavioural changes by the dogs on encountering the odour samples were recorded by video for later analysis. Consistent with our hypothesis, seizure-associated odours evoked behavioural changes in the dogs, concordant with attention seeking attempts and thereby supporting the view that seizures are accompanied by a distinctive odour.
Biochip With Multi-Planar Electrodes Geometry for Differentiation of Non-Spherical Bioparticles in a Microchannel
Authors: Farooq Amina , Butt Nauman , Hassan Umer 
Published: February 2021

Abstract
A biosensor capable of differentiating cells or other microparticles based on morphology finds significant biomedical applications. Examples may include morphological determination in the cellular division process, differentiation of bacterial cells, and cellular morphological variation in inflammation and cancer etc. Here, we present a novel integrated multi-planar microelectrodes geometry design that can distinguish a non-spherical individual particle flowing along a microchannel based on its electrical signature. We simulated multi-planar electrodes design in COMSOL Multiphysics and have shown that the changes in electrical field intensity corresponding to multiple particle morphologies can be distinguished. Our initial investigation has shown that top-bottom electrodes configuration produces significantly enhanced signal strength for a spherical particle as compared to co-planar configuration. Next, we integrated the co-planar and top-bottom configurations to develop a multi-planar microelectrode design capable of electrical impedance measurement at different spatial planes inside a microchannel by collecting multiple output signatures. We tested our integrated multi-planar electrode design with particles of different elliptical morphologies by gradually changing spherical particle dimensions to the non-spherical. The computed electrical signal ratio of non-spherical to spherical particle shows a very good correlation to predict the particle morphology. The biochip sensitivity is also found be independent of orientation of the particle flowing in the microchannel. Our integrated design will help develop the technology that will allow morphological analysis of various bioparticles in microfluidic channel in the future.
Natal Origin and Age-Specific Egress of Pacific Bluefin Tuna From Coastal Nurseries Revealed With Geochemical Markers
Authors: Rooker Jay R. , Wells R. J. David , Block Barbara A. , Liu Hui , Baumann Hannes , Chiang Wei-Chuan , Zapp Michelle Sluis , Miller Nathaniel R. , Mohan John A. , Ohshimo Seiji , Tanaka Yosuke , Dance Michael A. , Dewar Heidi , Snodgrass Owyn E. , Shiao Jen-Chieh 
Published: February 2021

Abstract
Geochemical chronologies were constructed from otoliths of adult Pacific bluefin tuna (PBT) to investigate the timing of age-specific egress of juveniles from coastal nurseries (natal sites) in the East China Sea or Sea of Japan to offshore waters of the Pacific Ocean. Element:Ca chronologies were developed for otolith Li:Ca, Mg:Ca, Mn:Ca, Zn:Ca, Sr:Ca, and Ba:Ca, and our assessment focused on the section of the otolith corresponding to the juvenile period (age-0 to age-1+). Next, we applied a common time-series approach (i.e., changepoint analysis) to geochemical profiles to identify divergences presumably linked to inshore-offshore migrations. Conspicuous geochemical shifts were detected during the juvenile interval for Mg:Ca, Mn:Ca, and Sr:Ca that were indicative of coastal-offshore transitions or egress generally occurring for individuals approximately 4-6 mo. old, with later departures (6 mo. or older) linked to overwintering being more limited. Changepoints in ototith Ba:Ca profiles were most common in the early age-1 period (ca. 12-16 mo.) and appear associated with entry into upwelling areas such as the California Current Large Marine Ecosystem following trans-Pacific migrations. Natal origin of PBT was also predicted using the early life portion of geochemical profile in relation to a baseline sample comprised of age-0 PBT from the two primary spawning areas in the East China Sea and Sea of Japan. Mixed-stock analysis indicated that the majority of adult PBT in our sample originated from the East China Sea, but individuals of Sea of Japan origin were also detected our sample of spawning adults collected in the Ryukyu Archipelago.