bluuebunny/crossref_metadata_2025_split huggingface dataset:
Zebrafish (Danio rerio) behavioural response to an odorant mixture containing attracting and repelling odorants
Authors: Shahriari Arash , Khara Lakhan S. , Allison W. Ted , Tierney Keith B. 
Published: February 2021

Abstract
Odorants guide essential activities such as foraging and fleeing predators. Studies usually examine behavioural and physiological effects of individual odorants, while animals in the environment are exposed to multiple stimuli simultaneously. In this study, we exposed zebrafish to a mixture of attraction-evoking and aversion-evoking amino acids, and behavioural responses were observed. Attraction to l-alanine and avoidance to l-cysteine were observed, and so these amino acids were used to make the mixture (zebrafish also avoided l-serine, but this was weaker than with l-cysteine exposures). When exposed to the mixture, fish responded with avoidance, which suggests that aversion-evoking stimuli outweigh attraction-evoking stimuli. Attraction towards the mixture was seen only when the concentration of l-cysteine was decreased from 0.1 to 0.001 mM. Olfactory ablation surgery confirmed that the behaviours were olfactory-mediated. Overall, this study demonstrated that odorant stimuli that repel outweigh stimuli that attract until their concentration decreases by as much as 100-fold.
From Social and Development Banking to Digital Financial Inclusion: the Journey of Banking in India
Authors: Sarkar Kaustav K. , Thapa Rukmini 
Published: February 2021

Abstract
The Indian banking and financial sector has endured a long journey from social and development banking to digital banking and financial inclusion. In recent years, there has been a growing consensus in support of digital financial inclusion. Several influential international entities argued strongly in favor of digitization of banking and expansion of digital finance. We tried to understand the journey of Indian banks through the lens of historical and economic changes, ranging from social and developmental banking to digital financial inclusion. In particular, the journey has been analyzed in the context of the changing role of the state, data-driven banking, and the infrastructural base required for a digital banking and payment system. Further, drawing experiences from other emerging nations, an attempt has been made to understand how far digital financial inclusion would be successful in India in reaching the unbanked and marginalized.
The Vanishing Point of World Literature
Authors: Landry Travis 
Published: February 2021

Abstract
In the field of world literature, questions about the temporal versus spatial nature of worlding persist. One reason is the problematic nature of this dichotomy. Taking up Pheng Cheah’s calls for the temporal worlding of indeterminate becoming, my paper complicates the fate of spatiality in this move toward ontological open-endedness. With discussion of the vanishing point, I postulate that imaginative distancing in its relation to memory involves a spatialization that need not be circumscribed by global flattening of the humanist ethos central to the worldliness of world literature. In response to the connection Cheah draws between literature and worldliness, I claim that the latter is characterized by a spatiotemporal elasticity born from the proliferation of meaning in the former. At the extreme, this movement gestures toward the infinite held by the vanishing point, and there, indeterminacy appears absurdly paradoxical in our search for world value, as consciousness loses itself in the repeated and necessary attempt to transcend distance that cannot be measured.
A prospective cohort study of the risk factors for new falls and fragility fractures in self-caring elderly patients aged 80 years and over
Authors: Zhou Jian , Liu Bo , Qin Ming-Zhao , Liu Jin-Ping 
Published: February 2021

Abstract
Background
This study aimed to prospectively analyze the risk factors for new falls and fragility fractures in self-caring elderly patients and to find suitable evaluation tools for community screening and follow-up interventions. Methods: A total of 300 participants (187 male and 113 female), aged 80 or above and capable of caring for themselves, were enrolled in this study and observed for a period of 12 months. Their medical histories were collected, various indicators were measured, and the risk factors for new falls and fragility fractures were analyzed.

Results
A total of 290 participants were included in the statistical analysis. Eighty-seven participants (30%) had new falls. The incidence was negatively correlated with the activities of daily living (ADL, represented by the Barthel Index) score (P=0.008) but was positively correlated with the timed up-and-go (TUG) test score> 12 s (P=0.021). The results also revealed that 33 fragility fractures occurred in 29 patients (10.0%), which was positively correlated with new falls (P=0.000). New fragility fractures were negatively correlated with the bone mineral density (BMD) of the lumbar vertebrae (P=0.012) and walking speed (P=0.000).

Conclusion
TUG, walking speed, the ADL score, and the fall risk assessment scale can simply and effectively assess the risk of new falls and fragility fractures in the elderly population, and their use should be widely implemented in the community.
Outcomes for 298 breastfed neonates whose mothers received ketamine and diazepam for postpartum tubal ligation in a resource-limited setting
Authors: Gilder Mary Ellen , Tun Nay Win , Carter Annabelle , Tan Ferdinand Frederik Som Ling , Min Aung Myat , Eh Hsa , Aye Pan , Carrara Verena I. , Angkurawaranon Chaisiri , McGready Rose 
Published: February 2021

Abstract
Background
Anesthesia in lactating women is frequently indicated for time-sensitive procedures such as postpartum tubal ligation. Ketamine and diazepam are two of the most commonly used anesthetic agents in low resource settings, but their safety profile in lactating women has not been established.

Methods
Medical records of post-partum tubal ligations between 2013 and 2018 at clinics of the Shoklo Malaria Research Unit were reviewed for completeness of key outcome variables. Logistic regression identified presence or absence of associations between drug doses and adverse neonatal outcomes: clinically significant weight loss (≥95th percentile) and neonatal hyperbilirubinemia requiring phototherapy.

Results
Of 358 records reviewed, 298 were lactating women with singleton, term neonates. There were no severe outcomes in mothers or neonates. On the first postoperative day 98.0% (290/296) of neonates were reported to be breastfeeding well and 6.4% (19/298) had clinically significant weight loss. Phototherapy was required for 13.8% (41/298) of neonates. There was no association between either of the outcomes and increasing ketamine doses (up to 3.8 mg/kg), preoperative oral diazepam (5 mg), or increasing lidocaine doses (up to 200 mg). Preoperative oral diazepam resulted in lower doses of intraoperative anesthetics. Doses of intravenous diazepam above 0.1 mg/kg were associated with increased risk (adjusted odds ratio per 0.1 mg/kg increase, 95%CI) of weight loss (1.95, 95%CI 1.13–3.35, p = 0.016) and jaundice requiring phototherapy (1.87, 95%CI 1.11–3.13, p = 0.017).

Conclusions
In resource-limited settings ketamine use appears safe in lactating women and uninterrupted breastfeeding should be encouraged and supported. Preoperative oral diazepam may help reduce intraoperative anesthetic doses, but intravenous diazepam should be used with caution and avoided in high doses in lactating women.
Survey on patients’ organisations’ knowledge and position paper on screening for inherited neuromuscular diseases in Europe
Authors:   ERN EURO-NMD Patient Advisory Board, Lamy F. , Ferlini A. , Evangelista Teresinha 
Published: February 2021

Abstract
Background
The development of new genetic testing methods and the approval of the first treatments raises questions regarding when and how to perform screening for inherited neuromuscular conditions. Screening directives and access to the different techniques is not uniform across Europe. The patient advisory board of the European reference network for rare neuromuscular diseases (NMD) conducted a qualitative study to understand the state of play of screening for inherited NMD in Europe and patients’ needs.

Results
We collected answers from 30 patient organisations (POs) from 18 European countries. Fifteen acknowledge the existence of pre-implantation genetic diagnosis in their country. Regarding prenatal screening, we had 25 positive answers and 5 negative ones. Twenty-four POs mentioned that newborn screening was available in their country. We had some contradictory answers from POs from the same country and in some cases; diseases said to be part of the screening programmes were not hereditary disorders. Twenty-eight organisations were in favour of screening tests. The reasons for the two negative answers were lack of reimbursement and treatment, religious beliefs and eventual insurance constrains. Most POs (21) were in favour of systematic screening with the option to opt-out. Regarding the timing for screening, “at birth”, was the most consensual response. The main priority to perform screening for NMDs was early access to treatment, followed by shorter time to diagnostic, preventive care and genetic counselling.

Conclusions
This is the first study to assess knowledge and needs of POs concerning screening for NMDs. The knowledge of POs regarding screening techniques is quite uneven. This implies that, even in communities highly motivated and knowledgeable of the conditions they advocate for, there is a need for better information. Differences in the responses to the questions “how and when to screen” shows that the screening path depends on the disease and the presence of a disease modifying treatment. The unmet need for screening inherited NMDs should follow an adaptive pathway related to the fast moving medical landscape of NMDs. International coordination leading to a common policy would certainly be a precious asset tending to harmonize the situation amongst European countries.
Treatment of acute exacerbation of liver-cirrhosis-associated portal vein thrombosis with direct-acting oral anticoagulant, edoxaban, used as an initial treatment in the early postoperative period after abdominal surgery: a case report
Authors: Toyoda Junya , Morioka Daisuke , Horii Nobutoshi , Nakayama Gakuryu , Oyama Norio , Asano Fumio , Izumisawa Yusuke , Miura Masaru , Sato Yoshiki , Endo Itaru 
Published: February 2021

Abstract
Background
Cirrhosis-associated portal vein thrombosis (CA-PVT) has been reportedly observed in 5–30% of cirrhotic patients. Moreover, the acute exacerbation of CA-PVT is likely to occur after certain situations, such as a status after abdominal surgery. Safety and efficacy of the direct-acting oral anticoagulant (DOAC) used for cirrhotic patients have been being confirmed. However, use of the DOAC as an initial treatment for CA-PVT appears still challenging especially in the early postoperative period after major surgery in terms of unestablished efficacy and safety in such occasion.

Case presentation
We herein report a case of the acute exacerbation of CA-PVT in the early postoperative period after abdominal surgery, which was successfully treated with DOAC, edoxaban used as an initial treatment. The patient was a 79-year-old Japanese male with alcoholic cirrhosis. The patient suffered choledocholithiasis and had a mural chronic CA-PVT extending from the superior mesenteric vein to the portal trunk. He underwent open cholecystectomy and choledochotomy. Early postoperative clinical course was uneventful except for abdominal distension due to ascites diagnosed on postoperative day (POD)7 when hospital discharge was planned. Contrast enhancement computed tomography (CE-CT) taken on POD 7 revealed the exacerbation of the CA-PVT. Despite recommendation for extension of hospital admission with low molecular weight heparin treatment, the patient strongly hoped to be discharged. Unwillingly, we selected DOAC, edoxaban, as an initial treatment, which was commenced the day after discharge (POD8). As a result, the remarkable improvement of the exacerbated CA-PVT was confirmed by the CE-CT taken on POD21. Any bleeding complications were not observed. Although a slight residue of the CA-PVT remains, the patient is currently doing well 4 years after surgery and is still receiving edoxaban. Any adverse effects of edoxaban have not been observed for 4 years.

Conclusions
A case of successful treatment of the acute exacerbation of CA-PVT with edoxaban was reported. Moreover, edoxaban has been safely administered in a cirrhotic patient for 4 years. The findings obtained from the present case suggest that DOAC can be used as an initial treatment for CA-PVT even in early postoperative period after major abdominal surgery.
Toxicological evaluations of betulinic acid and ursolic acid; common constituents of Houttuynia cordata used as an anthelmintic by the Naga tribes in North-east India
Authors: Mishra Vijaya , Soren Amar Deep , Yadav Arun Kumar 
Published: February 2021

Abstract
Background
Betulinic acid (BA) and ursolic acid (UA) are two major phytoconstituents of Houttuynia cordata Thunb., (Saururaceae) which is used as an anthelmintic in the traditional medicine system of the Nagas in Nagaland, India. This study evaluates their toxic potentials using rodent models (Swiss albino mice and Wistar rats) according to the OECD (Organization for Economic Cooperation and Development) guidelines. Acute and 28-day sub-acute oral toxicity studies were conducted, and evaluations were made based on biochemical, hematological, and histopathological observations.

Results
Acute oral toxicity study revealed the oral LD50 of both the test compounds to be > 2000 mg/kg in mice. Sub-acute administration of BA at 10 mg/kg body weight (b.w.) revealed a significant increase in serum glutamic oxaloacetic transaminase (SGOT), alkaline phosphatase (ALP), urea concentrations and eosinophil and lymphocyte counts in rats. Animals administered with 10 mg/kg b.w. UA revealed elevated neutrophil count, SGOT, ALP, and urea concentrations, whereas white blood cells (WBC), lymphocyte, and platelet counts were found to be low. Histopathological examinations of body organs revealed alterations in the architecture of the liver, kidney, and spleen tissues. Notably, all these alterations were recoverable as evident in the satellite group, indicating a recovering pattern from the toxic effects caused by the oral administration of these phytocompounds.

Conclusion
Although UA and BA possess several therapeutic properties, their long-term usage can cause mild toxicity in their users. This study also paves way for evaluating the optimum effective and safe dose of these phytocompounds.
Development and validation by statistical treatment of stability indicating RP-HPLC method for quantification of Orlistat in Orlistat-loaded solid dispersion
Authors: Sharma Kiran Singh , Sahoo Jagannath 
Published: February 2021

Abstract
Background
Most of the analytical methods reported for the estimation of Orlistat were complex, expensive, and deficient in reproducibility with no or very less informative regarding various statistical methods and equations used for the validation purpose. This study provides a fast, accurate, descriptive, and precise isocratic reversed phase high-performance liquid chromatographic (HPLC) method using Waters Spherisorb 5 μm Octadecyl-silica-2 (250 × 4.6 mm) column, for the estimation of Orlistat in bulk drug and pharmaceutical formulations with minimized drug extraction steps. The drug was detected in an analytical column with mobile phase comprising a mixture of methanol, acetonitrile, and 2% phosphoric acid in the ratio of 85:14:1 v/v/v at flow rate of 1 ml/min with elution monitoring at 215.0 nm.

Results
The retention time for Orlistat was found to be 5.9 min with sharp and proper peak. The linearity was covered over the concentration range of 1.00–10.00 μg/ml (r2 = 0.9997) with a limit of detection and limit of quantitation 0.06 and 0.2 μg/ml, respectively. The developed analytical technique was found to be validated for all the parameters within the acceptance criteria of ICH guidelines. The mean ± standard deviation (SD) recoveries of Orlistat were 99.87 ± 0.45.

Conclusion
The optimized method was well precise, accurate, sensitive, stability indicating, and tested with all statistical parameters. Thus, the method can be conveniently used in quality control and routine analysis of Orlistat containing solid dispersions and other formulations. The main advantage of the developed method was its high specificity for the estimation of Orlistat in presence of various degradation products resulting from stress conditions and formulation excipients.
HPLC method validation for quantification of tetrahydrocurcumin in bulk drug and formulation
Authors: Raut Roopam , Shaji Jessy 
Published: February 2021

Abstract
Background
Tetrahydrocurcumin (THC), the active metabolite of curcumin, is gaining popularity amongst scientist due to its wide spectrum of pharmacological activities, better stability and colourless nature. The objective of this study was to develop a sensitive, cost-effective RP-HPLC method for the estimation of THC in bulk drug substance and formulation.

Results
Efficient chromatographic separation was achieved on Hypersil BDS, C18 column, 250 mm × 4.6 mm, 5 μm column by isocratic elution with mobile phase comprising of acetonitrile: methanol: water (40:23:37% V/V); adjusted to a pH of 3.0 ± 0.05. The flow rate of the mobile phase was 1.0 ml/min with a column temperature of 25 °C. UV detector was used for the analysis and detection was carried out at 280 nm. The developed method was validated according to ICH guidelines with respect to system suitability, linearity, accuracy, precision and robustness. The theoretical plates were found to be more than 5800. The method showed linearity over the range of 4 to 60 μg/ml with R2 = 0.9998. The accuracy of the method in terms of recovery study was 98.23-99.99%. The %RSD for intra-day and inter-day precision were 0.272 and 0.275, respectively. The method was found to be robust with respect to change in wavelength, flow rate and column temperature.

Conclusion
The analytical method was found satisfactory on validation as per ICH guidelines. Hence, it can be routinely used for quantification of THC in bulk drug and formulation.
Association of Patient Experience With Guideline-Concordant Colon Cancer Treatment in the Elderly
Authors: Snyder Rebecca A. , Wardrop Rebecca , McLain Alexander C. , Parikh Alexander A. , Cass Anna L. 
Published: February 2021

PURPOSE: The association between patient-reported experience of care and care quality is not well described. The purpose of this study was to assess the relationship between the patient-reported experience and receipt of guideline-concordant colon cancer (CC) treatment. METHODS: Medicare beneficiaries with resected stage I-III CC (2003-2013) were identified in the linked SEER Consumer Assessment of Healthcare Providers and Systems data set. Patient-reported scores were compared by receipt of guideline concordant care (GCC) (resection of ≥ 12 lymph nodes [stage I-III] and adjuvant chemotherapy [stage III]). Linear mixed-effects regression was performed to compare adjusted mean patient experience scores by GCC. RESULTS: Of the 1,010 identified patients, 58.4% of stage I (n = 192/329) and 73.4% of stage II (n = 298/406) patients underwent resection of ≥ 12 LN. Among stage III patients, 76.0% (n = 209/275) underwent resection of ≥ 12 lymph node and 52.4% (n = 144/275) received adjuvant chemotherapy. By multivariable analysis, patient-reported scores of healthcare quality, physicians, physician communication, getting needed care, and getting care quickly were similar among patients who received GCC compared with those who did not. However, mean scores of overall healthcare quality (91.3 v 82.4, P = .0004) and getting needed care (92.8 v 86.8, P = .047) were higher among stage III patients who received GCC compared with those who did not. CONCLUSION: Patient-reported scores of healthcare quality and ability to get needed care are associated with GCC among elderly patients with stage III CC. Further investigation is needed to determine whether patient-reported experience correlates with other clinical measures of quality of CC care.
¿Cómo hacer factibles los derechos fundamentales de otras especies?
Authors: Sánchez Cárdenas Diego Alejandro 
Published: February 2021

Una de las cuestiones que ha motivado mayor exclusión en la protección brindada por los derechos fundamentales es la obsesión por caracterizar al titular de derechos. Hasta la actualidad, existe un enfoque subjetivo de los derechos fundamentales, bajo el cual, las conductas jurídicamente trascendentes son aquellas que lesionan en sus bienes básicos a sujetos con cualidades específicas: “seres dignos”. Lo que lastimosamente excluye del mundo jurídico a seres distintos al ser humano.
Frente a esta situación, el presente trabajo propone una reordenación de las “funciones jurídicas” de los sistemas de derechos fundamentales. De esta manera, con el ánimo de incluir en el desarrollo jurídico a otras formas de vida, se postulará que la primera función de los sistemas jurídicos debe ser seleccionar como relevante - sin mayor discriminación - a toda aquella conducta que: i) sea debatida socialmente (bajo determinadas características) y; ii) esté relacionada con la supervivencia, el sufrimiento, la libertad y, en general, la satisfacción de las necesidades más elementales (en los términos que se expondrán).
Lo anterior, no supone que el sistema jurídico no deba realizar un análisis ontológico del sujeto, sino que el mismo, lejos de excluir a ciertos seres del mundo jurídico, se desempeñe - como una segunda función del sistema jurídico - con el objeto de determinar la regulación que se brindará a determinado comportamiento que es previamente aceptado como trascendente en la esfera jurídica, debido a su importancia social y sus características especiales.
Con lo anterior, se espera brindar un enfoque que haga factibles los derechos de los animales y del medio ambiente; así como sustentar teóricamente los esbozos que ya han reconocido a especies diferentes al ser humano como intereses jurídicos independientes.
A Theory of Assurance: Balancing Costly Formal Control with Tone at the Top
Authors: Penno Mark 
Published: February 2021

Informal tone at the top (TATT) is widely regarded as a fundamental ingredient of organizational control, yet because of its soft nature, the scholarship on TATT has not emphasized mathematical modeling. Observing that TATT refers to the example set by top management, I model TATT as, ceteris paribus, reflecting the expectation that the chief executive officer (CEO) will not divert organizational resources for personal benefit. The setting assumes that CEOs are randomly drawn from a labor pool containing candidates who either value career prospects (high type) or who do not value career prospects (low type). The fear of dismissal and a damaged career mean that high types tend to be less opportunistic than low types, who divert for personal gain. Contrary to the belief that trust and vulnerability go hand in hand, I identify conditions where TATT and optimal costly formal controls are complements. But I also show in the same setting that TATT and optimal formal controls can be substitutes. The results also explain why high levels of TATT may not be welcomed by the external auditor or certain stakeholders and explain ambiguous Sarbanes-Oxley reports. This paper was accepted by Suraj Srinivasan, accounting.