bluuebunny/crossref_metadata_2025_split huggingface dataset:
STING Protects Against Cardiac Dysfunction and Remodelling by Blocking Autophagy
Authors: Xiong Rui , Li Ning , Liu Bohao , He Ruyuan , Jiang Wenyang , Wang Wei , Wang Bo , Geng Qing 
Published: January 2021

Abstract
Background: Heart failure, which is characterized by cardiac remodelling, is one of the most common chronic diseases in the aged. Stimulator of interferon genes (STING) acts as an indispensable molecule modulating immune response and inflammation in many diseases. However, the effects of STING on cardiomyopathy, especially cardiac remodelling are still largely unknown. This study was designed to investigate whether STING could affect cardiac remodelling and to explore the potential mechanisms. Methods: In vivo, aortic binding (AB) surgery was performed to construct the mice model of cardiac remodelling. A DNA microinjection system was used to trigger STING overexpression in mice. The STING mRNA and protein expression levels in mice heart were measured, and the cardiac hypertrophy, fibrosis, inflammation and cardiac function were also evaluated. In vitro, cardiomyocytes stimulated by Ang II and cardiac fibroblasts stimulated by TGF-β to performed to further study effects of STING on cardiac hypertrophy and fibroblast. In terms of mechanisms, the level of autophagy was detected in mice challenged with AB. Rapamycin, a canonical autophagy inducer, intraperitoneal injected into mice to study possible potential pathway.Results: In vivo, the STING mRNA and protein expression levels in mice heart challenged with AB for 6 weeks were significantly increased. STING overexpression significantly mitigated cardiac hypertrophy, fibrosis and inflammation, apart from improving cardiac function. In vitro, experiments further disclosed that STING overexpression in cardiomyocytes induced by Ang II significantly inhibited the level of cardiomyocyte cross-section area and the ANP mRNA. Meanwhile, TGF-β-induced the increase of α-SMA content and collagen synthesis in cardiac fibroblasts could be also blocked by STING overexpression. In terms of mechanisms, mice challenged with AB showed higher level of autophagy compared with the normal mice. However, STING overexpression could reverse the activation of autophagy triggered by AB. Rapamycin, a canonical autophagy inducer, offset the cardioprotective effects of STING in mice challenged with AB. Finally, further experiments unveiled that STING may inhibit autophagy by phosphorylating ULK1 on serine757.Conclusion: STING may prevent cardiac remodelling induced by pressure overload by inhibiting autophagy, which could be a promising therapeutic target in heart failure.
Usefulness of Intraoperative C-Arm Image Intensifier in Reducing Errors of Acetabular Component During Primary Total Hip Arthroplasty: An Application of Widmer’s Method
Authors: Song Joo-Hyoun , Kim Yong-Sik , Kwon Soon-Yong , Lim Young-Wook , Jung Jiyoung , Oh Seungbae 
Published: January 2021

Abstract
Background: Acetabular prosthesis positioning in total hip arthroplasty (THA) is crucial in reducing the risk of dislocation. There has been minimal research on the proper way to put the acetabular components into the safe zone intraoperatively. Assessment of version by intraoperative imaging intensifier is very valuable. The value of Widmer’s method, using the intraoperative C-arm available to determine cup anteversion was assessed.Methods: 101 hips in 91 patients who underwent primary THA were eligible for inclusion. Utilizing intraoperative C-arm images, measurement was performed using the technique described by Widmer. The values obtained using 3D computed tomography postoperatively, which determined the anteversion of the acetabular component, were regarded as the reference standard.Results: The method of Widmer obtained values similar to those obtained using 3D computed tomography and was considered accurate (n.s.). All 101 hips were positioned in the set target zone. Among the 101 hips, the cup position in nine hips (8.9%) was changed. The dislocation rate in our study was 1.0% with all dislocations occurring in hips placed in the target zone. The mean Harris hip score after THA in one year was 94.2 (82-98).Conclusions: The method of Widmer was accurate using intraoperative imaging intensifier for the measurement of the anteversion of the acetabular component during THA, with reference to the anteversion obtained from the 3D computed tomography. Also, utilizing intraoperative C-arm imaging was very useful because it allowed for correction of the position of the acetabular cup.
Breast lesions excised via Vacuum-Assisted System: Could We Get Any Clues for B3 Lesions Before Excision Biopsy?
Authors: Zheng Liang , Zheng Fufu , Xing Zhaomin , Zhang Yunjian , Li Yongxin , Xu Hongbiao , Lai Yuanhui , Li Jie , Wang Wenjian 
Published: January 2021

Abstract
Background: The purpose of this study was to determine the validity of the ultrasound features as well as patient characteristics assigned to B3 (uncertain malignant potential) breast lesions before vacuum-assisted excision biopsy (VAEB).Methods: This study population consisted of 2245 women with breast-nodular abnormalities, which were conducted ultrasound-guided VAEB (US-VAEB). Patient’s clinical and anamnestic data and lesion-related ultrasonic feature variables of B3 were compared with those of benign or malignant cases, using histopathological results as a benchmark. Results: The proportions of benign, B3 and malignant breast lesions were 88.5%, 8.2% and 3.4% respectively. B3 high frequent occurred in BI-RADS-US grade 3 (7.7%), grade 4 (9.3%), grade 4a (11.3%) and grade 4b (9.1%). The overall malignancy underestimation rate of B3 cases was 4.4% (8/183). The more common malignancy underestimation of B3 subtypes were PL/ADH 50% (1/2), ADH 23.1% (3/13), and CSL/RS 16.7% (1/6). And the highly frequent recurrence rates of B3 subtypes were ADH 10% (1/10), PL 4.9% (4/82), and PT 8.6% (6/70). Multivariate binary logistic regression analyses (B3 vs benign) showed that non-menopausal patients (95% CI 1.628-8.616, P = 0.002), single (95% CI 1.370-2.650, P = 0.000) or abundant blood supply (95% CI 1.745-4.150, P = 0.000) nodules via ultrasound examination were significant risk factors for B3 occurrences. Moreover, patients elder than 50 years (95% CI 3.178-19.816, P = 0.000), unclear margin (95% CI 3.571-14.119, P = 0.000) or microcalcification (95% CI 4.010-30.733, P = 0.000) nodules were significantly associated with higher risks of malignant potentials (B3 vs malignant).Conclusion: The results of this study indicate that ultrasound findings and patients’ characteristics could provide valuable information for distinguishing B3 cases from benign or malignant cases before VAEB.
Association Between Violent Video Gaming Exposure and the Attachment Styles Among Lebanese Male Adolescents
Authors: Akel Marwan , Berro Jana , Malaeb Diana , Fahs Iqbal , Haddad Chadia , Hallit Souheil , Obeid Sahar 
Published: January 2021

Abstract
Objectives: to correlate different attachment styles with the exposure of Lebanese male adolescents to violent video games.Methods: This study was carried out between October and December 2019 among 388 Lebanese male adolescents from five Lebanese schools. Results: 190 participants (48.8%, CI: 0.43 – 0.53) were classified as having a high exposure to violent video gaming. Higher age (Beta=-1.64) and higher dismissing attachment style (Beta=-1.38) were significantly associated with less violent video gaming. Higher fearful attachment style (Beta=2.98) was significantly associated with more violent video gaming.Conclusion: This study focuses on the association between different attachment styles and the exposure of Lebanese male adolescents to violent video games. Adolescents with more insecure attachment styles were found to be more exposed to violent video games.
Systematic Expression Analysis of the Diagnostic and Prognostic Value of HEPACAM Family Member 2 in Colon Adenocarcinoma
Authors: Wang Shuai , Ruan Guo-Tian , Gong Yi-Zhen , Liao Cun , Zhang Lei , Gao Feng 
Published: January 2021

Abstract
Background: The diagnostic and prognostic value of HEPACAM family member 2 (HEPACAM2) gene in patients with colon adenocarcinoma (COAD) is rarely reported. Therefore, the purpose of this study is to explore the diagnostic and prognostic value of HEPACAM2 gene in patients with COAD.Methods: Firstly, we analyzed the differential expression levels of HEPACAM2 gene and diagnostic value analysis from different databases. Secondly, univariate and multivariate survival analysis of the prognostic value of HEPACAM2 gene in patients with COAD was performed. Finally, utilizing joint-effects analysis and comprehensive prognosis analysis to investigate the prognostic value of HEPACAM2 and related genes.Results: Differential analyses of multiple databases showed that the HEPACAM2 expression level in COAD tumor tissue was significantly lower than that of adjacent normal tissues. The diagnostic ROC curve results indicated that HEPACAM2 gene had a higher diagnostic value in COAD. The RT-qPCR verification results of COAD tissue in the Guangxi cohort showed that HEPACAM2 expression level in COAD tumor tissue was significantly higher than that of adjacent normal tissues (P<0.001), and the diagnostic value was high in COAD (AUC=0.892). The prognostic value analysis showed that low expression of HEPACAM2 gene had a poorer prognosis of overall survival (OS) in patients with COAD when compared with those patients with high expression of HEPACAM2 gene ((P = 0.038, HR (95% CI) = 0.635(0.414-0.976)). Joint-effects analysis and comprehensive prognosis analysis showed that high expression of HEPACAM2 combined with high expression of CLCA1, high expression of REP15, and high expression of B3GNT6 were associated with a better prognosis of overall COAD OS.Conclusion: The results of this study suggested that the HEPACAM2 may be an independent diagnostic and prognostic biomarker for COAD.
Associations Between Telomere Length and Insulin Resistance in American Adults：A Cross-Sectional Study from the US National Health and Nutrition Examination Survey, 1999–2002
Authors: Gong Rongpeng , Li Zheng , Liu Ya , Luo Gang , Yang Lixin , Wei Xiaoxing 
Published: January 2021

Abstract
BackgroundSome previous studies seemed to confirm a link between telomere length and many chronic diseases, but in recent years, a growing body of evidence has shown a weak link between telomere length and some chronic diseases. In previous studies insulin resistance has been listed as a sign of arterial aging, diabetes, and especially atherosclerosis. However, there is insufficient evidence that telomere length is associated with insulin resistance in US adults. Therefore, we tried to find evidence of telomere length and insulin resistance based on the NHANES1999-2002 database.MethodsWe used multiple logistic regression analysis to test the relationship between telomere length and insulin resistance, and drew a smooth fitting curve between telomere length and insulin resistance without adjustment. At the same time, groups of people were divided according to age, gender, race, etc., and multivariate Logistic analysis was conducted after adjusting covariables to test the association between telomere length and insulin resistance in different groups.ResultsIn this study, after the adjustment of covariables. Telomere length was not associated with insulin resistance in U.S. adults and was not statistically significant.ConclusionsSince there is a significant association between insulin resistance and race, further investigation and study is needed to determine whether telomere length is related to the risk of insulin resistance in other populations. At the same time, more research is needed to explore the link between telomeres and chronic disease in the future.
Unveiling the 46,XY disorder of sex development patients family with a novel mutation in the GATA4 gene
Authors: Li Liwei , Yang Hui , Zhang Junhong , Lv Huijing , Li Qing , Cui Yi , Wu Tongqian , Tian Yuan , Mou Yajun , Chen Xinxin , Wang Shuping , Feng Yanli , Li Shujun , Li Shuping , Hu Pingsheng , Hao Shirui , Dou Xiaowei 
Published: January 2021

Abstract
Background

GATA-binding protein 4 (GATA4) is the critical regulator in gonadal development and its mutation has been reported related with 46,XY disorder of sex development (DSD). Here, we found the two Chinese cases with 46,XY DSD carried the GATA4 mutation. Physical examinations, B-ultrasound and Karyotype analysis were performed and confirmed the two patients with 46,XY DSD.
Results

Sequencing were performed and the heterozygous mutation p.Gly375Arg in GATA4 gene was identified in the 2 cases with 46,XY DSD. Their mother was identified carrying the p.Gly375Arg mutation in GATA4 protein. However, their father and litter sister without 46,XY DSD didn’t be found carrying the p.Gly375Arg mutation in GATA4 gene.
Conclusion

This is the first report that the case with 46,XY DSD carried the mutation Gly375Arg in the GATA4 gene. Our
Finding the Context Indigenous Innovation in Village Enterprise Knowledge Structure: A Topic Modeling
Authors: Kusumastuti Retno , Silalahi Mesnan , Asmara Anugerah Yuka , Hardiyati Ria , Juwono Visnhu 
Published: January 2021

Abstract
Indigenous people have deep local knowledge of environmental sustainability and natural resource utilization, which are sources of innovations that often are drivers for economic growth in rural areas. This study explores the knowledge structure of indigenous innovation in village enterprises through content analysis of research publications. The resulting knowledge structure can be used to set up a roadmap for the studies on village enterprise and in a broader context to build metadata as a foundation for an evaluation system of village enterprise. The authors deploy topic modeling and co-word analyses to scrutinize 775 village enterprise research articles from the Scopus database and 665 paper from ScienceDirect. In the topic modeling, topic models village enterprises are setup. The topics found are local ownership (such as market and property), land, services (housing, health care), economy and public policy, financial service micro-credit, environmental pollution control, local business sustainability, social entrepreneurship, and household income, bioenergy based electrification, and bumdes management. Four sectors of the natural resource-based indigenous economy were identified: traditional food production, bio-energy for fuel and electricity, agriculture, and tourism. The topic models are used to comprehend knowledge structure in the village enterprises whereby the focus is to uncover the context of indigenous village enterprise and its states of the art.
Is the Effect of Intervertebral Foramen Endoscopic Surgery on Adjacent Segmental Degeneration Better Than That of Open Surgery?
Authors: shunmin wang , sun kaiqiang , liu aigang , chen rongzi , luo xi , cao jie , zhao feng , shi jiangang 
Published: January 2021

Abstract
Background To compare the clinical outcomes of transforaminal lumbar interbody fusion (TLIF) and endoscopic discectomy in the treatment of postoperative adjacent segment degeneration in patients with lumbar disc herniation. Methods From 2014 to 2017, 87 patients who were diagnosed with single-level lumbar disc herniation (LDH) and received surgery of TLIF (group X, n = 43) or endoscopic discectomy (group F, n = 44) were retrospectively analyzed. X-ray, MRI, CT and clinical symptoms were recorded before operation and at the last follow-up. The neurological function was originally evaluated by the (JOA) score of the Japanese Orthopaedic Association. Radiological evaluation included the height of intervertebral space (HIS), intervertebral foramen height (FH), intervertebral foramen area (FA), lumbar lordosis (CA) and intervertebral disc degeneration pfirrmann score.Results There was no significant difference in average operation age, JOA improvement rate, reoperation rate and complications between the two groups. The average blood loss, average hospital stays and average operation time in group F were lower than those in group X. During the last follow-up, HIS, CA and FA decreased in both groups, and the changes in group X were more significant than those in group X (P < 0.05). There was no significant difference in postoperative FH between the two groups, but it decreased more in group X (P < 0.05).Conclusion Both TLIF and endoscopic surgery can achieve good results in the treatment of LDH, but the risk of lumbar adjacent segment degeneration after intervertebral foraminal surgery is lower.
Expression of N6-Methyladenosine (m6A) Regulators Correlates With Immune Microenvironment Infiltration and Predicts Prognosis in Diffuse Large Cell Lymphoma (DLBCL)
Authors: Xie Zucheng , Li Meiwei , Hong Haoyuan , Xu Qingyuan , He Zhendong , Peng Zhigang 
Published: January 2021

Abstract
Background: N6-methyladenosine (m6A) and immune microenvironment infiltration have been widely reported to play important roles in various cancers. However, in diffuse large cell lymphoma (DLBCL), the clinical significance of m6A regulators and their relationship with immune microenvironment infiltration have not been illuminated.Methods: The expression of m6A regulators in DLBCL was investigated using The Cancer Genome Atlas and Genotype-Tissue Expression databases. The capacity of m6A regulators in dividing molecular clusters of DLBCL was determined using Consensus Clustering algorithm and validated via principal component analysis. The clinical traits and prognosis difference in m6A-sorted clusters were revealed. The m6A prognostic signature was established and validated based on Gene Expression Omnibus dataset using univariate Cox and LASSO regression analysis. The immune cell infiltration and the expression of immune checkpoint genes in m6A low/high-risk DLBCL were studied. Gene set enrichment analysis (GSEA) was adopted to unveil the underlying molecular mechanism in m6A low/high-risk DLBCL.Results: Differentially expressed m6A regulators were able to molecularly discriminate DLBCL as two clusters based on consensus clustering and principal component analysis. A six m6A regulators-based risk prediction signature was established and validated as an independent predictor, which separated patients into m6A low- and high-risk groups. High-risk m6A indicates worse survival, for which the predictive AUC at 1-, 2-, and 5-year achieved 0.605, 0.640, and 0.652, respectively. Immune cell infiltration analysis revealed the B cells naïve and T cells gamma delta were the top increased and decreased immune cells in high-risk m6A patients. Up-regulated (PDCD1 and KIR3DL1) and down-regulated (TIGIT, DO1, and BTLA) immune checkpoint genes in high-risk m6A patients were identified. GSEA analysis unveiled high-risk m6A related to tumor proliferation associated process, while low-risk m6A related to defense response associated process. Conclusions: This study provided a comprehensive analysis for the clinical significance of m6A regulators and their association with immune microenvironment infiltration. An m6A regulators-based risk signature may be applied for the risk stratification of DLBCL patients, thus may facilitate the clinical management of DLBCL. Immune microenvironment was found to be closely related to m6A risk, which may be part of the mechanism of m6A regulators in DLBCL.
ISSI 2020: Mechanism and Method of Testing Fracture Toughness and Impact Absorbed Energy By Spherical Indentation Tests
Authors: Li Jianxun , Zhang Tairui , Wang Shang , Cheng Jirui , Wang Weiqiang 
Published: January 2021

Abstract
Aimed at the problem that conventional approaches for mechanical property determination all need destructive sampling, which may be improper for in-service structures, the authors proposed a method to determine the quasi-static fracture toughness and impact absorbed energy from spherical indentation tests (SITs) in this study. The stress status and damage mechanism of SIT, Mode I fracture, Charpy impact tests, and related tests were first investigated through finite element (FE) calculations and scanning electron microscope (SEM) observations, respectively. It was found that the damage mechanism of SITs is different from Mode I fracture, while the Mode I fracture and Charpy impact test share the same damage mechanism. Taking the difference between SIT and Mode I fracture into consideration, the uniaxial tension and pure shear were introduced to correlate SIT with Mode I fracture. Based on which, the widely used critical indentation energy (CIE) model in fracture toughness determination from SITs was modified. The quasi-static fracture toughness determined from the modified CIE model was used in evaluating the impact absorbed energy by means of the dynamic fracture toughness and energy to crack initiation. Effectiveness of the new proposed method was verified through experiments on four kinds of steels, i.e. Q345R, SA508-3, 18MnMoNbR, and S30408.
Research On Energy Saving and Performance Enhancement of High-End Hydraulic Press With Controllable Accumulator
Authors: Hua Lin , Xu Zhicheng , Liu Yanxiong , Zhao Xinhao 
Published: January 2021

Abstract
Hydraulic fineblanking press is a kind of high-end hydraulic metal forming devices and widely applied in automotive and appliance industry. However, it suffers from the defeat of high energy consumption low energy efficiency. To solve the problem, this study proposed a power-matching strategy by using a novel controllable accumulator which can control the precharge pressure, output flow with high precision. Firstly, the energy characteristics and working performance requirements of the large-sized fineblanking press in a working cycle were investigated. Then, the energy consumption mathematic model coupling with the controllable accumulator was built for designing the key parameters of the accumulators. Based on the load characteristic and the energy model, a controlling strategy of the controllable accumulator was proposed to reduce the imbalance degree of the supplied and demanded power and improve working performance by designing working route of the controllable accumulator. Finally, a detailed hydraulic schematic was designed and applied on the 1000 ton hydraulic fineblanking press, which was validated with simulation model. The results show that compared to the traditional system, the energy efficiency of the novel system is improved by 20.35% with lowering the input energy by 169.4 kJ. Besides, the vibration magnitude of the slide block is decreased a lot and the working production efficiency is improved by 10% compared to the traditional system.