bluuebunny/crossref_metadata_2025_split huggingface dataset:
Development of the caudal-fin skeleton reveals multiple convergent fusions within Atherinomorpha
Authors: Thieme Philipp , Warth Peter , Moritz Timo 
Published: April 2021

AbstractBackgroundThe caudal fin of teleosts is a highly diverse morphological structure and a valuable source of information for comparative analyses. Within the Atherinomorpha a high variation of conditions of the caudal-fin skeleton can be found. These range from complex but basal configurations to simple yet derived configurations. When comparing atherinomorph taxa, it is often difficult to decide on the homology of skeletal elements if only considering adult specimens. However, observing the development of caudal-fin skeletons allows one to evaluate complex structures, reveal homologies and developmental patterns, and even reconstruct the grundplan of the examined taxa.ResultsWe studied the development of the caudal-fin skeleton in different atheriniform, beloniform and cyprinodontiform species using cleared and stained specimens. Subsequently we compared the development to find similarities and differences in terms of 1) which structures are formed and 2) which structures fuse during ontogeny. For many structures, i.e., the parhypural, the epural(s), the haemal and neural spines of the preural centra and the uroneural, there were either no or only minor differences visible between the three taxa. However, the development of the hypurals revealed a high variation of fusions within different taxa that partly occurred independently in atheriniforms, beloniforms and cyprinodontiforms. Moreover, comparing the development of the ural centra exposed two ways of formation of the compound centrum: 1) in atheriniforms and the beloniformsOryziasandHyporhamphus limbatustwo ural centra develop and fuse during ontogeny while 2) in cyprinodontiforms and Exocoetidae (Beloniformes) only a single ural centrum is formed during ontogeny.ConclusionsWe were able to reconstruct the grundplan of the developmental pattern of the caudal-fin skeleton of the Atheriniformes, Beloniformes and Cyprinodontiformes as well as their last common ancestors. We found two developmental modes of the compound centrum within the Atherinomorpha, i.e., the fusion of two developing ural centra in atheriniforms and beloniforms and the development of only one ural centrum in cyprinodontiforms. Further differences and similarities for the examined taxa are discussed, resulting in the hypothesis that the caudal-fin development of a last common ancestor to all atherinomorphs is very much similar to that of extant atheriniforms.
Analyzing efforts to synergize the global health agenda of universal health coverage, health security and health promotion: a case-study from Ethiopia
Authors: Tadesse Amare Worku , Gurmu Kassu Ketema , Kebede Selamawit Tesfaye , Habtemariam Mahlet Kifle 
Published: April 2021

Abstract
Background
Evidence exists about synergies among universal health coverage, health security and health promotion. Uniting these three global agendas has brought success to the country’s health sector. This study aimed to document the efforts Ethiopia has made to apply nationally synergistic approaches uniting these three global health agendas. Our study is part of the Lancet Commission on synergies between these global agendas.

Methods
We employed a case study design to describe the synergistic process in the Ethiopian health system based on a review of national strategies and policy documents, and key informant interviews with current and former policymakers, and academics. We analyzed the “hardware” (using the World Health Organization’s building blocks) and the “software” (ideas, interests, and power relations) of the Ethiopian health system according to the aforementioned three global agendas.

Results
Fragmentation of health system primarily manifested as inequities in access to health services, low health workforce and limited capacity to implementation guidelines. Donor driven vertical programs, multiple modalities of health financing, and inadequate multisectoral collaborations were also found to be key features of fragmentation. Several approaches were found to be instrumental in fostering synergies within the global health agenda. These included strong political and technical leadership within the government, transparent coordination, and engagement of stakeholders in the process of priority setting and annual resource mapping. Furthermore, harmonization and alignment of the national strategic plan with international commitments, joint financial arrangements with stakeholders and standing partnership platforms facilitated efforts for synergy.

Conclusions
Ethiopia has implemented multiple approaches to overcome fragmentation. Such synergistic efforts of the primary global health agendas have made significant contributions to the improvement of the country’s health indicators and may promote sustained functionality of the health system.
HANDY: a device for assessing resistance to mechanical crushing of maize kernel
Authors: Su Yuan , Xu Yang , Cui Tao , Gao Xiaojun , Xia Guoyi , Li Yibo , Qiao Mengmeng , Yu Yingbo 
Published: April 2021

AbstractBackgroundHow to control the physical damage during maize kernel harvesting is a major problem for both mechanical designers and plant breeders. A limitation of addressing this problem is lacking a reliable method for assessing the relation between kernel damage susceptibility and threshing quality. The design, construction, and testing of a portable tool called “HANDY”, which can assess the resistance to mechanical crushing in maize kernel. HANDY can impact the kernel with a special accelerator at a given rotating speed and then cause measurable damage to the kernel. These factors are varied to determine the ideal parameters for operating the HANDY.ResultsBreakage index (BI, target index of HANDY), decreased as the moisture content of kernel increased or the rotating speed decreased within the tested range. Furthermore, the HANDY exhibited a greater sensitivity in testing kernels at higher moisture level influence on the susceptibility of damage kernel than that in Breakage Susceptibility tests, particularly when the centrifugation speed is about 1800 r/min and the centrifugal disc type is curved. Considering that the mechanical properties of kernels vary greatly as the moisture content changes, a subsection linear (average goodness of fit is 0.9) to predict the threshing quality is built by piecewise function analysis, which is divided by kernel moisture. Specifically, threshing quality is regarded as a function of the measured result of the HANDY. Five maize cultivars are identified with higher damage resistance among 21 tested candidate varieties.ConclusionsThe HANDY provides a quantitative assessment of the mechanical crushing resistance of maize kernel. The BI is demonstrated to be a more robust index than breakage susceptibility (BS) when evaluating threshing quality in harvesting in terms of both reliability and accuracy. This study also offers a new perspective for evaluating the mechanical crushing resistance of grains and provides technical support for breeding and screening maize varieties that are suitable for mechanical harvesting.
PredCRG: A computational method for recognition of plant circadian genes by employing support vector machine with Laplace kernel
Authors: Meher Prabina Kumar , Mohapatra Ansuman , Satpathy Subhrajit , Sharma Anuj , Saini Isha , Pradhan Sukanta Kumar , Rai Anil 
Published: April 2021

AbstractBackgroundCircadian rhythms regulate several physiological and developmental processes of plants. Hence, the identification of genes with the underlying circadian rhythmic features is pivotal. Though computational methods have been developed for the identification of circadian genes, all these methods are based on gene expression datasets. In other words, we failed to search any sequence-based model, and that motivated us to deploy the present computational method to identify the proteins encoded by the circadian genes.ResultsSupport vector machine (SVM) with seven kernels, i.e., linear, polynomial, radial, sigmoid, hyperbolic, Bessel and Laplace was utilized for prediction by employing compositional, transitional and physico-chemical features. Higher accuracy of 62.48% was achieved with the Laplace kernel, following the fivefold cross- validation approach. The developed model further secured 62.96% accuracy with an independent dataset. The SVM also outperformed other state-of-art machine learning algorithms, i.e., Random Forest, Bagging, AdaBoost, XGBoost and LASSO. We also performed proteome-wide identification of circadian proteins in two cereal crops namely,Oryza sativaandSorghum bicolor, followed by the functional annotation of the predicted circadian proteins with Gene Ontology (GO) terms.ConclusionsTo the best of our knowledge, this is the first computational method to identify the circadian genes with the sequence data. Based on the proposed method, we have developed an R-package PredCRG (https://cran.r-project.org/web/packages/PredCRG/index.html) for the scientific community for proteome-wide identification of circadian genes. The present study supplements the existing computational methods as well as wet-lab experiments for the recognition of circadian genes.
Drug dealing and drug use prevention – a qualitative interview study of authorities’ perspectives on two open drug scenes in Stockholm
Authors: Feltmann Kristin , Gripenberg Johanna , Strandberg Anna K. , Elgán Tobias H. , Kvillemo Pia 
Published: April 2021

Abstract
Background
The use of illicit substances causes various health and social problems globally. Moreover, open drug use and dealing in urban areas, i.e., open drug scenes, can cause public order problems, lead to the recruitment of marginalized and young people for drug use or sale, and induce feelings of insecurity in the general public. Although some international studies have described various ways to manage open drug scenes, such as law enforcement and social interventions, there is limited knowledge about the facilitators and barriers promoting or impeding the implementation of such interventions. The aim of the current study was to explore how different authorities describe the nature of two open drug scenes in Stockholm and to derive authorities’ perspective on facilitators and barriers to implementing interventions to reduce open drug dealing, drug use, and related problems at these locations.

Methods
Semi-structured interviews with police officers, security staff, social workers, and municipality officials (n = 21) in the municipality of Stockholm were conducted and analyzed by qualitative content analysis.

Results
The analysis of the interviews generated the following categories: Problems, Interventions, Organizational factors, and External factors, revealing information about the strategic and daily counteracting work occurring at the open drug scenes as well as authorities’ perceptions of facilitators and barriers to implementing interventions to reduce open drug dealing, drug use, and related problems. Facilitators included motivated and skilled professionals and organized collaboration between key actors. Prominent barriers were a lack of resources to maintain personnel continuity at the scenes, policies that impede information sharing and put security staff in danger, and people who use or sell drugs without having residence permits.

Conclusions
To increase the possibility of successful implementation of interventions to counteract open drug dealing, politicians and authorities should pay attention to collaboration between key actors, sufficient resource allocation, possible modification of policy governing professional duties, and remedies to the vulnerability of individuals without residence permits.
Results of the CHlorhexidine Gluconate Bathing implementation intervention to improve evidence-based nursing practices for prevention of central line associated bloodstream infections Study (CHanGing BathS): a stepped wedge cluster randomized trial
Authors: Reynolds Staci S. , Woltz Patricia , Keating Edward , Neff Janice , Elliott Jennifer , Hatch Daniel , Yang Qing , Granger Bradi B. 
Published: April 2021

Abstract
Background
Central line-associated bloodstream infections (CLABSIs) result in approximately 28,000 deaths and approximately $2.3 billion in added costs to the U.S. healthcare system each year, and yet, many of these infections are preventable. At two large health systems in the southeast United States, CLABSIs continue to be an area of opportunity. Despite strong evidence for interventions to prevent CLABSI and reduce associated patient harm, such as use of chlorhexidine gluconate (CHG) bathing, the adoption of these interventions in practice is poor.
The primary objective of this study was to assess the effect of a tailored, multifaceted implementation program on nursing staff’s compliance with the CHG bathing process and electronic health record (EHR) documentation in critically ill patients. The secondary objectives were to examine the (1) moderating effect of unit characteristics and cultural context, (2) intervention effect on nursing staff’s knowledge and perceptions of CHG bathing, and (3) intervention effect on CLABSI rates.

Methods
A stepped wedged cluster-randomized design was used with units clustered into 4 sequences; each sequence consecutively began the intervention over the course of 4 months. The Grol and Wensing Model of Implementation helped guide selection of the implementation strategies, which included educational outreach visits and audit and feedback. Compliance with the appropriate CHG bathing process and daily CHG bathing documentation were assessed. Outcomes were assessed 12 months after the intervention to assess for sustainability.

Results
Among the 14 clinical units participating, 8 were in a university hospital setting and 6 were in community hospital settings. CHG bathing process compliance and nursing staff’s knowledge and perceptions of CHG bathing significantly improved after the intervention (p = .009, p = .002, and p = .01, respectively). CHG bathing documentation compliance and CLABSI rates did not significantly improve; however, there was a clinically significant 27.4% decrease in CLABSI rates.

Conclusions
Using educational outreach visits and audit and feedback implementation strategies can improve adoption of evidence-based CHG bathing practices.

Trial registration
ClinicalTrials.gov, NCT03898115, Registered 28 March 2019.
5335 days of Implementation Science: using natural language processing to examine publication trends and topics
Authors: Scaccia Jonathan P. , Scott Victoria C. 
Published: April 2021

Abstract
Introduction
Moving evidence-based practices into the hands of practitioners requires the synthesis and translation of research literature. However, the growing pace of scientific publications across disciplines makes it increasingly difficult to stay abreast of research literature. Natural language processing (NLP) methods are emerging as a valuable strategy for conducting content analyses of academic literature. We sought to apply NLP to identify publication trends in the journal Implementation Science, including key topic clusters and the distribution of topics over time. A parallel study objective was to demonstrate how NLP can be used in research synthesis.

Methods
We examined 1711 Implementation Science abstracts published from February 22, 2006, to October 1, 2020. We retrieved the study data using PubMed’s Application Programming Interface (API) to assemble a database. Following standard preprocessing steps, we use topic modeling with Latent Dirichlet allocation (LDA) to cluster the abstracts following a minimization algorithm.

Results
We examined 30 topics and computed topic model statistics of quality. Analyses revealed that published articles largely reflect (i) characteristics of research, or (ii) domains of practice. Emergent topic clusters encompassed key terms both salient and common to implementation science. HIV and stroke represent the most commonly published clinical areas. Systematic reviews have grown in topic prominence and coherence, whereas articles pertaining to knowledge translation (KT) have dropped in prominence since 2013. Articles on HIV and implementation effectiveness have increased in topic exclusivity over time.

Discussion
We demonstrated how NLP can be used as a synthesis and translation method to identify trends and topics across a large number of (over 1700) articles. With applicability to a variety of research domains, NLP is a promising approach to accelerate the dissemination and uptake of research literature. For future research in implementation science, we encourage the inclusion of more equity-focused studies to expand the impact of implementation science on disadvantaged communities.
Overweight in Swedish show dogs–prevalence and association with performance in competition
Authors: Lindåse Sanna , Feltenmark Tilda , Krantz Malin , Söder Josefin 
Published: April 2021

AbstractBackgroundThe prevalence of overweight and obesity is increasing in companion dogs, but little is known of these conditions in show dogs. This study assessed body condition score (BCS) of show dogs of six selected popular breeds at a major Swedish dog show event and examined the association between BCS and performance in competition.ResultsAt one of Sweden’s largest dog shows, BCS of 120 dogs of six different breeds was assessed by trained animal healthcare personnel, using a 9-point BCS scale with conditional cut-off for overweight set to BCS ≥ 6. Prevalence of overweight in the cohort was 32% but all overweight dogs except one displayed only slight overweight (BCS 6) and no dog was assessed as obese (BCS 8–9). Prevalence of overweight differed significantly between breeds (P < 0.0001) with Labrador retrievers, Golden retrievers and French bulldogs showing the highest mean BCS (5.6–5.7) and highest prevalence of overweight (50–67%). Lean and overweight dogs received awards and higher show awards (certificates) to the same extent, and no significant association between slight overweight and performance in competition was found.ConclusionsPrevalence of overweight in Swedish show dogs was relatively high and in the same range as in the Swedish dog population as a whole. Dog owners, breeders and judges should be made aware of canine obesity problems and trained in BCS assessment, to better prevent canine overweight and associated health risks. This is particularly important for retriever and brachycephalic breeds, which showed high prevalence of slight overweight and have breed-specific health problems exacerbated by overweight. Owners and breeders of traditionally sturdy dog breeds should be informed that overweight dogs do not outperform lean dogs in competition.
Investigating the effects of substrate morphology and experimental conditions on the enzymatic hydrolysis of lignocellulosic biomass through modeling
Authors: Rohrbach Jessica C. , Luterbacher Jeremy S. 
Published: April 2021

Abstract
Background
Understanding how the digestibility of lignocellulosic biomass is affected by its morphology is essential to design efficient processes for biomass deconstruction. In this study, we used a model based on a set of partial differential equations describing the evolution of the substrate morphology to investigate the interplay between experimental conditions and the physical characteristics of biomass particles as the reaction proceeds. Our model carefully considers the overall quantity of cellulase present in the hydrolysis mixture and explores its interplay with the available accessible cellulose surface.

Results
Exploring the effect of various experimental and structural parameters highlighted the significant role of internal mass transfer as the substrate size increases and/or the enzyme loading decreases. In such cases, diffusion of cellulases to the available cellulose surface limits the rate of glucose release. We notably see that increasing biomass loading, while keeping enzyme loading constant should be favored for both small- (R < 300 $$\mu m$$

μ
m

) and middle-ranged (300 < R < 1000 $$\mu m$$

μ
m

) substrates to enhance enzyme diffusion while minimizing the use of enzymes. In such cases, working at enzyme loadings exceeding the full coverage of the cellulose surface (i.e. eI>1) does not bring a significant benefit. For larger particles (R > 1000 $$\mu m$$

μ
m

), increases in biomass loading do not offset the significant internal mass transfer limitations, but high enzyme loadings improve enzyme penetration by maintaining a high concentration gradient within the particle. We also confirm the well-known importance of cellulose accessibility, which increases with pretreatment.

Conclusions
Based on the developed model, we are able to propose several design criteria for deconstruction process. Importantly, we highlight the crucial role of adjusting the enzyme and biomass loading to the wood particle size and accessible cellulose surface to maintain a strong concentration gradient, while avoiding unnecessary excess in cellulase loading. Theory-based approaches that explicitly consider the entire lignocellulose particle structure can be used to clearly identify the relative importance of bottlenecks during the biomass deconstruction process, and serve as a framework to build on more detailed cellulase mechanisms.
Proliferative diabetic retinopathy in patients with type 2 diabetes correlates with the presence of atherosclerosis cardiovascular disease
Authors: Gao Lu , Zhao Wei , Yang Jin-Kui , Qin Ming-Zhao 
Published: April 2021

Abstract
Background
Atherosclerosis cardiovascular disease (ASCVD) is the main cause of morbidity and mortality in type 2 diabetes mellitus (T2DM). As most diabetic patients with ASCVD are asymptomatic, it is most neglected in clinical practice. For this reason, identifying high-risk ASCVD population with intensified treatment is very important. In recent years, the relationship between diabetic retinopathy (DR) and ASCVD has caused much academic concern, but the results are inconsistent. Moreover, whether all grades of DR increase the risk of ASCVD remains controversial. Most importantly, very few data can be found in China.

Objective
Our aim is to discuss whether all grades of DR increase the risk of ASCVD after adjustment for the traditional cardiovascular risk factors and to assess the independent contribution of DR to cardiovascular events in patients with T2DM, hoping to provide more evidence for early identification of ASCVD.

Research design and methods
A total of 425 T2DM patients with complete physical and biochemical data were included in the study. The grade of DR was assessed with two 45 color digital retinal images. Based on the presence of history of ASCVD, 425 T2DM patients were divided into 2 groups: ASCVD group and non-ASCVD group.

Results
ASCVD patients were older and had a significantly higher fasting plasma glucose (FPG) and glycated haemoglobin (HbA1c) and proportion of history of ASCVD. At the same time, they were more likely to be females, and had lower level of alcohol and calculated glomerular filtration rate (eGFR) than non-ASCVD patients. Their trend to develop DR with ASCVD was significantly higher than patients with non-ASCVD (χ2 = 5.805, P  = 0.016). DR was an independent statistical indicator of the presence of ASCVD [odds ratio (OR) (95% CI): 2.321 (1.152–4.678), P = 0.018]. Furthermore, when DR was divided into non-proliferative retinopathy (NPDR) and proliferative retinopathy (PDR) according to its severity, only PDR was significantly associated with incident ASCVD [OR (95% CI): 8.333 (1.813–38.304), P = 0.006]. After adjusting for traditional ASCVD risk factors, such an association still existed [OR (95% CI): 7.466 (1.355–41.137), P = 0.021].

Conclusion
DR associates strongly with ASCVD in the Chinese population with T2DM. With the increasing severity of DR, the risk of ASCVD also increases. After adjustment for traditional risk factors, PDR is still an independent risk marker for ASCVD.