bluuebunny/crossref_metadata_2025_split huggingface dataset:
Qingwei San Treats Oral Ulcer Subjected to Stomach Heat Syndrome in db/db Mice by Targeting TLR4/MyD88/NF-κB Pathway
Authors: Shi Lu , An Yongcheng , Cheng Long , Li Yiyang , Li Huimin , Wang Chen , Lv Yinglan , Duan Yuhui , Dai Hongyu , He Changhao , Zhang Huilin , Huang Yan , Fu Wanxin , Wang ShengPeng , Zhao Baosheng , Wang Yitao , Zhao Yonghua 
Published: November 2021

Abstract
Background Qingwei San (QWS), one of classic Chinese Medicine prescripts, has been widely used to treat stomach heat syndrome which manifests oral ulcer (OU), periodontitis and upper gastrointestinal bleeding for seven hundred years. However, the therapeutic effects of QWS on diabetic OU subjected to stomach heat syndrome are still ambiguous. In the study, we investigate the pharmacological mechanisms. Methods The main components of QWS aqueous extract were analyzed by LC-MS, and potential pathways of QWS targeting OU were predicted by network pharmacology. The db/db mice were administered with the decoction of dried Zingiber officinale Rosc. rhizome combined with NaOH cauterization to establish the model of diabetic OU subjected to stomach heat syndrome. Subsequently, the model mice were treated with QWS, and OU wound healing status were recorded. The pathological changes of gastric tissue and oral mucosa were evaluated using hematoxylin-eosin staining, and the morphology of collagen fibers in oral mucosa was assessed by Masson staining. The levels of thromboxane B 2 (TXB 2 ), 6-Keto-prostaglandin F1α (6-keto-PGF1α), interleukin-1 β (IL-1β), IL-2, IL-6, tumor necrosis factor-α (TNF-α), β-endorphin (β-EP) and 5-Hydroxytryptamine (5-HT) were determined by ELISA assay. The protein expressions of Toll-like receptor 4 (TLR4), TNF receptor associated factor 6 (TRAF6), myeloid differentiation factor 88 (MyD88), inhibitor of NF-κB alpha (IκΒα), p-IκΒα and nuclear factor kappa-B (NF-κB) p65 were measured by Western Blotting. Results A total of 183 compounds in QWS were identified by LC-MS, and identified 79 bioactive compounds corresponded to 269 targets and 59 pathways. QWS high-dose treatment significantly reduced the level of TXB 2 and the ratio of TXB 2 /6-keto-PGF1α. Meanwhile, it improved mucosal pathological morphology, and reduced the area of OU and local edema. Simultaneously, the levels of TNF-α, IL-1β, IL-6, IL-2 and 5-HT, and the expressions of TLR4、TRAF6、MyD88、p-IκΒα and NF-κB p65 were decreased. Conclusion QWS treatment facilitates the healing of OU, ameliorates pathological morphologies of gastric and oral mucosa and decreases the levels of pro-inflammatory cytokines in db/db mice subjected to stomach heat syndrome, whose mechanism may be associated with the inhibition of TLR4/MyD88/NF-κB signaling pathway to exert anti-inflammatory effects.
Construction of a Convenient Screening Method for P-Hydroxybenzoate Hydroxylase To Enable Efficient Gallic Acid and Pyrogallol Biosynthesis
Authors: Chen Zhenya , Chen Tongtong , Yu Shengzhu , Huo Yi-Xin 
Published: November 2021

Abstract
BackgroundGallic acid (GA) and pyrogallol are phenolic hydroxyl compounds and have diverse biological activities. Microbial-based biosynthesis of GA and pyrogallol has been emerged as an ecofriendly method to replace the traditional chemical synthesis. In GA and pyrogallol biosynthetic pathways, the low hydroxylation activity of p-hydroxybenzoate hydroxylase (PobA) towards 3,4-dihydroxybenzoic acid (3,4-DHBA) limited the high-level biosynthesis of GA and pyrogallol.ResultsThis work reported a high active PobA mutant (Y385F/T294A/V349A PobA) towards 3,4-DHBA. This mutant was screened out from a PobA random mutagenesis library through a novel naked eye visual screening method. In vitro enzyme assay showed this mutant has a kcat/Km of 0.059 μM-1s-1 towards 3,4-DHBA, which was 4.92-fold higher than the reported mutant (Y385F/T294A PobA). Molecular docking simulation provided the mechanism explanation of the high activity. Expression of this mutant in E. coli BW25113 (F’) can generate 830 ± 33 mg/L GA from 1000 mg/L 3,4-DHBA. After that, we utilized this mutant to assemble a de novo GA biosynthetic pathway. Subsequently, this pathway was introduced into a 3,4-DHBA-producing strain (E. coli BW25113 (F’)ΔaroE) to achieve 301 ± 15 mg/L GA production from simple carbon sources. Similarly, assembling this mutant into a de novo pyrogallol biosynthetic pathway enabled 129 ± 15 mg/L pyrogallol production.ConclusionsThis work established an efficient screening method and generated a high active PobA mutant. Assembling this mutant into GA and pyrogallol biosynthetic pathways achieved the de novo production of these two compounds. Besides, this mutant has great potential for GA or pyrogallol derivatives production. The screening method could be used for other GA biosynthesis-related enzymes.
Effects of polycyclic aromatic hydrocarbons on the proliferation and differentiation of placental cells
Authors: Jo Yun Sung , Ko Hyun Sun , Kim Ah Young , Jo Ha Gyeong , Kim Woo Jung , Choi Sae Kyung 
Published: November 2021

Abstract
Background
The purpose of this study was to investigate the effects of polycyclic aromatic hydrocarbons (PAHs) other than bisphenol A (BPA) and BPA substitutes on placental cells.
Methods
HTR-8/SVneo cells were treated with anthracene, benzo[k]fluoranthene, benzo[a]pyrene, endocrine disruptors, and 4,4-(9-fluorenylidene)diphenol, which is used as a substitute for BPA-free products. After confirming the dose response for each reagent using the prepared cells, the cells were incubated for 24, 48, and 72 h. Cell viability was confirmed using the XTT assay. Each experiment was performed with the minimum number of samples (n = 3) required for statistical analysis. The results were analyzed using t-tests; p < 0.05 was considered statistically significant.
Results
After treatment with anthracene, benzo[k]fluoranthene, benzo[a]pyrene, endocrine disruptors, and 4,4-(9-fluorenylidene)diphenol, the absorbance measured using the XTT assay decreased significantly with increasing concentration. The absorbance decreased significantly over time following treatment with each endocrine disruptor at the concentration confirmed by the dose–response analysis.
Conclusions
This study showed that anthracene, benzo[k]fluoranthene, benzo[a]pyrene, and 4,4-(9-fluorenylidene)diphenol—a BPA substitute—affect cell viability and necrosis in the placental cell line. The study indicates the serious effects of PAHs that negatively affect pregnancy but were previously unknown. Further, this study would serve as a reference for the identification of harmful PAHs during pregnancy prognosis in women who are more susceptible to PAH exposure.
A Novel Pyroptosis-related Genes Prognostic Signature Affects the Immune Infiltration Patterns in Hepatocellular Carcinoma
Authors: Jin Yiqun , Xue-song Bai. 
Published: November 2021

Abstract
PurposePyroptosis is an inflammatory form of cell death associated with tumorigenesis and progression. However, the prognostic value of pyroptosis-related genes (PRGs) in hepatocellular carcinoma (HCC) have not been elucidated.MethodsWe downloaded mRNA expression profiles and clinical information from TCGA and ICGC database. Then, differently expressed PRGs were screened to construct a multigene prognostic signature by least absolute contraction and selection operator (LASSO) Cox regression method in TCGA cohort. Date from ICGC was used to validate the robustness of this signature. Kaplan-Meier analysis was used to compare overall survival (OS) between high- and low-risk group. Univariate and multivariate Cox analysis were performed to identify the independent prognostic value of the signature. Gene set enrichment analysis (GSEA) was utilized to conduct GO and KEGG analysis. Single-sample gene set enrichment analysis was implemented to assess the immune cell infiltration and immune-related function. TIDE algorithm evaluated the significance of this signature in predicting immunotherapeutic sensitivity. ResultsAn 8-PRGs prognostic model was established. The OS of low-risk group was significantly increased compared to high-risk group. Receiver operating characteristic curve showed the model had a good prognostic predictive accuracy. Cox regression analysis proved the model an independent predictor for OS in HCC. GSEA indicated that the risk score was associated with immune response. Furthermore, different subgroups exhibited different immunoinfiltration patterns, different immune-checkpoint levels and different potential responses for immune-checkpoint blockade therapy.ConclusionAn 8-PRGs signature can predict the prognosis of HCC patients and may act as an immunotherapeutic potential target for HCC.
Biochemical Targets and Molecular Mechanism of Ginsenoside Compound K for Treating Osteoporosis Based on Network Pharmacology
Authors: Zhang Sen , Shen Shihong , Ma Pei , Fan Daidi 
Published: November 2021

Abstract
Ginsenosides have been proven to be potential beneficial in treatment of osteoporosis. To investigate the potential of ginsenosides in osteoporosis, ginsenoside compound K (GCK) was selected to explore the potential therapy targets and mechanism based on network pharmacology (NP). 206 and 6590 targets were obtained for GCK and osteoporosis, respectively, in which 138 targets were identified as co-targets of GCK and osteoporosis based on intersection analysis. Five central gene clusters and hub genes (STAT3, PIK3R1, VEGFA, JAK2 and MAP2K1) were identified through protein-protein interaction network analysis. Gene Ontology (GO) enrichment implied that phosphatidylinositol-related biological process, molecular modification and function may play an important role for GCK in treatment and prevention of osteoporosis. Functions and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis suggested that 16 targets were enriched in the osteoclast differentiation. Also, except for being identified as hub targets, MAPK and phosphatidylinositol-related proteins were enriched in the downstream signaling of c-Fms in the osteoclast differentiation pathway. Molecular docking further confirmed that GCK could interact with active cavity on the surface of c-Fms (osteoclast differentiation-related membrane receptor), and their complex could be stabilized by three H-bonds with residues including Glu 664 (3.19 Å), Glu 664 (2.62 Å) and Cys 666 (2.78 Å). Summarily, GCK could interfere the occurrence and progress of osteoporosis through c-Fms-mediated MAPK and phosphatidylinositol-related signaling regulating osteoclast differentiation.
Lateral Trans-Malleolar Approach for Posterior Malleolar Fractures of the Ankle for Articular and Syndesmosis Reduction : A Retrospective Study
Authors: Taki Masanori , Suzuki Kosuke , Hio Naohiro , Hasegawa Atsushi 
Published: November 2021

Abstract
Background
The lateral transmalleolar approach (LTA) is a surgical approach for posterior malleolar fractures (PMFs), providing direct visualization of the articular surface of the posterior malleolus, though temporarily disrupting the syndesmosis. However, it is still unclear whether this fibrous joint can be effectively reconstructed afterward to prevent complications. We report the clinical results and computed tomography (CT) findings for articular and syndesmosis reduction status after surgery with LTA for PMF.
Methods
Sixteen patients who underwent PMF reduction with LTA and were followed up for at least 1 year were evaluated retrospectively. According to the AO/OTA classification, the fracture type was type 44B in 13 patients and 44C in three patients. The Haraguchi classifications of PMF were type 1 in six cases and type 2 in 10 cases. The American Orthopedic Foot and Ankle Society Ankle-Hindfoot Scale (AOFAS) score, post-surgical complications, and reduction status of PMF and syndesmosis on CT were investigated.
Results
The mean AOFAS score was 93.0 ± 5.2 points. Post-operative complications were observed in one case of superficial infection and one case of delayed fibular union. The articular step-off seen on CT improved significantly after surgery (5.9 ± 2.9 mm preoperatively vs. 0.6 ± 0.8 mm post-operatively). Syndesmosis malreduction was seen in three cases, including two high fibular fractures and one comminuted fibular fracture with osteoporosis.
Conclusions
The LTA provided favourable clinical results and effective reduction of intra-articular PMFs. However, surgeons should be aware of the possibility of syndesmosis malreduction in cases of high fibular fractures or comminuted fibular fractures.
Synthesis and Characterization of Non-Stoichiometric Li1.1Co0.3Fe2.1O4 Ferrite Nanoparticles For Humidity Applications
Authors: ElFarhatey Ebtesam Ateia , Ateia Mahmoud A. , Mosry M.M. , Singh Charanjeet , Arman M.M. 
Published: November 2021

Abstract
Humidity sensor plays a crucial role in determining the efficiency of materials and the precision of apparatuses. To measure and control humidity, a non-stoichiometric Li1.1Co0.3Fe2.1O4 mesopores sensor is synthesized by a modified citrate auto combustion technique. The XRD study confirms that prepared nanoparticles are cubic spinel structures having Fd3m space group. The crystallite size is approximate 36 nm. Thermal analyses measurements confirm that the samples become thermally stable starting from 600 °C. Additionally, the kinetic studies of the prepared samples are calculated via a pseudo-first-order kinetic model. The temperature dependence of AC conductivity is found to increase with increasing the temperature. These observations are explained in various models. The resistivity mechanism of humidity sensors is studied via Complex impedance spectroscopy (CIS). Its impedance data is fitting to a corresponding circuit, to achieve a simulation of the sample under study. This fitting is detected by the Nyquist plot (Cole-Cole). The obtained data confirms that the studied samples are very sensitive to humidity and can be commercially used as a humidity sensing element.
Economic Growth and Quality Adjusted Human Capital Equation: Moderating Role of Social Capabilities in Africa
Authors: Joseph Tonuchi Emmanuel , Obikaonu Pauline Chimuru 
Published: November 2021

Abstract
The role of human capital on economic growth across countries has over time garnered lots of discussion in economic literature. This is fundamental, given that the actual determinant of the difference in income per capita across countries or why some countries are growing faster than other countries has remained an unresolved issue. This study provides a different insight into the nexus between human capital and economic growth by accounting for the role of social capabilities in a panel framework. Specifically, the study covers 40 African countries between 1998-2019, where the General Method of Moment (GMM) was employed to estimate the model. Specifically, it was discovered that without improved legal institutions and better economic opportunities, human capital impact on the growth of income per capita across countries is insignificant though positive. The study concludes that the effectiveness of knowledge accumulation and adoption of technology in a country is hinged on the availability of an enhanced legal, social, and economic environment.
WITHDRAWN: Performance Analysis of Optimal Sized Hybrid Renewable Energy Grid-Connected Systems.
Authors: Prajapati Anjani Kumar , Srivast Sudhir Kumar 
Published: November 2021

Abstract
Hybrid solar energy-based power generation systems (PGS) are one of the exciting options for potential distributed networks. PV and wind grid linked PGSs are the most appropriate for its good output across various configurations. However, due to the system's complexity, special attention is required to achieve a successful engineering solution in the optimal balance between these two energy sources. This paper discusses optimum scale of PV and wind by following multiple optimization methods to various condition decision analysis (VCDA). The versatility of the VCDA algorithm was tested by taking into account several weighting parameter techniques for differing wind speeds and fluctuations in radiation levels, thus illustrating the advantages and limitations of the suggested optimum size approaches. The subsequent study may be called as a significant reference for decision makers, analysts and policymakers.
WITHDRAWN: A Novel Approach for Mitigating Power Quality Issues in a PV Integrated Microgrid System Using an Improved Jelly Fish Algorithm
Authors: Suman Swati , Chatterjee Debashis , Mohanty Rupali 
Published: November 2021

Abstract
A two-step methodology was used to address the power quality concerns for the PV integrated microgrid system. In the first step, partial shading was included to deal with the real-time issues. The Improved Jelly Fish Algorithm integrated Perturb and Obserb (IJFA-PO) has been proposed to track the Global Maximum Power Point (GMPP). In the second step, the main unit powered by a DC-AC converter is synchronised with the grid. To cope with the wide voltage variation, an auxiliary unit is connected to the main unit in the opposite phase. This study evaluates various switching approaches to determine the optimal solution for achieving the stated goals. It was found that the IJFA-based SHE in 120 օ conduction gives improved results. A novel series compensation technique has been employed to further eliminate harmonics before grid integration. The proposed IJFA has been used to determine the switching angles for the SHEPWM converter. The objective function and novel series compensation work together to determine the harmonics that should be decreased and kept to a minimum. Three switching angles' performance was equivalent to that of nine switching angles. As a result, higher-order harmonics can be minimised with fewer switches, and switching losses can be lowered without compromising efficiency. The THD of the proposed system was 1.32 percent, which is very much within the tolerable limit of IEEE 1547, IEC, and CIGRE WG 36-05 standards. In terms of efficiency, metaheuristics, and convergence, the proposed system outperformed the existing ones. The model was developed in MATLAB/Simulink 2016b. To verify the simulation results, an experimental prototype of grid synchronised PV capacity of 260W was tested under various loading conditions. The present model is reliable and features a simple controller that provides more convenient and adequate performance for practical reasons.
A Dynamic K-means Based Clustering Algorithm Using Fuzzy Logic for CH Selection and Machine Learning Based Data Transmission
Authors: Choudhary Anupam , Badholia Abhishek , Sharma Anurag , Patel Brijesh , Jain Sapna 
Published: November 2021

Abstract
Clustering is effective method to increase network lifetime, energy efficiency, and connectivity of Sensor nodes in wireless sensor network. An energy efficient clustering algorithm has been proposed in this paper. Sensor nodes are clustered using K-means algorithm which dynamically forms number of clusters in accordance with number of alive nodes. Selection of suitable CH is done by fuzzy inference system by choosing three fuzzy input variable such as residual energy of Sensor node, its distance from cluster center and base station. Amount of data transmitted by member nodes to CH is reduced by machine learning that classify similar data at regular interval. The simulation results show that proposed algorithm outperforms other cluster based algorithms in terms of data received by base station, number of alive node per round, time of first node, middle node and last node to die for various density of sensor nodes and scalable conditions.
Long Term Surgical Oncological and Functional Outcome of Large Petroclival and Cerebellopontine Angle Epidermoid Cysts: A Multicenter Study.
Authors: Sellier Aurore , Troude Lucas , Baumgarten Clément , Caudron Yohan , Bretonnier Maxime , Gallet Clémentine , Boissonneau Sébastien , Cungi Pierre-Julien , Morandi Xavier , Dufour Henry , Fournier Henri-Dominique , Gay Emmanuel , Kalamarides Michel , Roche Pierre-Hugues 
Published: November 2021

Abstract
Objective: To assess the long-term surgical results on cranial nerve (CN) function and tumor control in patients harboring cerebellopontine angle (CPA) and petroclival area (PCA) epidermoid cysts (EC).Methods: This is a retrospective cohort study about 56 consecutive patients operated on for a CPA or PCA EC between January 2001 and July 2019 in six participating French cranial base referral centers.Results: Sixteen patients (29%) presented a PCA EC, and 40 a CPA EC (71%). The median clinical and radiological follow-up was 46 months (range 0-409). Preoperative CN disorders were present in 84% of patients (n=47), 72% of them experienced CN deficits improvement at last follow-up consultation (n=34) : 60% of cochlear and vestibular deficits (n=9/15 in both groups), 67% of trigeminal neuralgia (n=10/15), 53% of trigeminal hypoesthesia (n=8/15), 44% of lower cranial nerve disorders (n=4/9), 38% of facial nerve deficits (n=5/8), and 43% of oculomotor deficits (n=3/7) improved or were cured after surgery. New postoperative CN deficits occurred in 48% of patients (n=27). Most of them resolved at last follow-up, except for cochlear deficits which improved in only 14% of cases (n = 1/7). Twenty-six patients (46 %) showed evidence of tumor progression after a median duration of 63 months (range 7-210). Extent of resection, tumor location and tumor size were not associated with the occurrence of new postoperative CN deficit nor tumor progression. Conclusion: A functional nerve-sparing resection of posterior fossa EC is an effective strategy to optimize the results on preexisting CN deficits and reduces the risk of permanent de novo deficits.
A Rare Case of Iliopsoas Abscess in a Term Neonate – Case Report and Brief Review of Literature
Authors: Peddu Sai Praveen , Nanda Debasish , Pradhan Antaryami , Kumar TV Ram 
Published: November 2021

Abstract
Iliopsoas abscesses are rare in neonates. Clinical presentation of neonates with iliopsoas abscess often mimic other common neonatal illness. Typical clinical features as described in adults may not be observed in neonates. Such abscesses cause a diagnostic dilemma for the clinician, often resulting in a delay in clinical diagnosis and institution of specific treatment. We report a case of a 10-day old term male neonate from community who presented with high grade fever, abdominal distension, left lower limb swelling with limitation of movement and diagnosed to have left sided iliopsoas abscess on ultrasonography. The neonate undergone and extraperitoneal surgical drainage of the abscess along with a course systemic antimicrobial agent. The case is of clinical importance because it describes the presence of a localized abscess in an uncommon location and Methicillin resistant staphylococcus being the causative organism, which represents a rare and potentially life-threatening infection in neonates.