bluuebunny/crossref_metadata_2025_split huggingface dataset:
Bacterial Contamination of Healthcare worker’s Mobile Phones; a Case Study at Two Referral Hospitals in Uganda
Authors: Fred Tusabe 
Published: October 2021

Abstract
Background Hospital and Community-acquired infections are escalating and pose significant public health unhealthiness worldwide. The advancements of telemedicine and automation of healthcare records are supported by cellphones, laptops and wearable devices. This study focused on the incidence of healthcare workers’ mobile phones becoming contaminated with pathogenic bacteria and their possible roles as vehicles of transmission of antimicrobial-resistant bacteria.Method: A case study at two referral hospitals in Uganda between May and October 2020. Self-administered questionnaires were administered to participants after informed consent. Mobile phones of the participants in different departments of the hospitals were swabbed and samples were collected and transported to the microbiology laboratory for bacterial culture and antimicrobial susceptibility tests. Results: The point prevalence of Healthcare workers’ mobile phone bacterial contamination with one or more species was 93%. Organisms isolated were E. coli 5.6% (1), Micrococcus spp 11.1% (2), Coagulase-negative staphylococci, CoNS, 61.1% (11) and Bacillus spp 22.2% (4). About 45% of the organisms were multidrug-resistant. Resistance was major to penicillin, cotrimoxazole, ciprofloxacin and Gentamycin respectively. The isolated E. coli was resistant to all antibiotics used in the study. Only 15% (2) of the participants disinfected their phones at least once a week and 8% cleaned their hands after using a mobile phone.Conclusion: Healthcare Workers’ mobile phones can act as fomites for the transmission of multidrug-resistant micro-organisms. This study provides strong evidence for developing and strengthening disinfection protocols for mobile phones and does not underscore the importance of hand hygiene in the middle of a patient encounter especially when the HCW grabs a phone but doesn't re-clean their hands before patient contact.
Combinatorial G x G x E CRISPR screening and functional analysis highlights SLC25A39 in mitochondrial GSH transport
Authors: Shi Xiaojian , Reinstadler Bryn , Shah Hardik , To Tsz-Leung , Byrne Katie , Summer Luanna , Calvo Sarah , Goldberger Olga , Doench John , Mootha Vamsi , Shen Hongying 
Published: October 2021

Abstract
The SLC25 carrier family consists of 53 transporters that shuttle nutrients and co-factors across mitochondrial membranes1-3. The family is highly redundant and their transport activities are coupled to metabolic state. Here, we introduce a pooled, dual CRISPR screening strategy that knocks out
pairs of transporters in four metabolic states — glucose, galactose, OXPHOS inhibition, and absence of pyruvate — designed to unmask the inter-dependence of these genes. In total, we screened 63 genes in four metabolic states, corresponding to 2016 single and pair-wise genetic perturbations. We recovered 19 gene-by-environment (GxE) interactions and 9 gene-by-gene (GxG) interactions. One GxE interaction hit illustrated that the fitness defect in the mitochondrial folate carrier (SLC25A32) KO cells were genetically buffered in galactose due to a lack of substrate in de novo purine biosynthesis. Another GxE interaction hit revealed non-equivalence of the paralogous ATP/ADP exchangers (ANTs) with ANT2 specifically required during OXPHOS inhibition. GxG analysis highlighted a buffering interaction between the iron transporter SLC25A37 and the poorly characterized SLC25A39. Mitochondrial metabolite profiling, organelle transport assays, and structure-guided mutagenesis suggest SLC25A39 is critical for mitochondrial glutathione (GSH) transport. Our work underscores the importance of systematically investigating family-wide genetic interactions between mitochondrial transporters across many metabolic environments.
Becoming a wage earned or a self-employed: Does Young Educational Level determine the Choice of the Working Labour Force Status in Cameroon?
Authors: Maximilien KOUL NGWE MANGUELLE 
Published: October 2021

Abstract
If employment is recognized as a key driver for growth, development and well-being improvement, human capital is probably a main determinant of the labour force participation. By often analyzing this relationship in wage earning jobs, studies used to leave self-employment untreated despite the fact that its proportion is growing significantly in several countries. This leads us to the following question: does human capital accumulated determine access to all forms of employment? Focusing on young people, this article analyzes effects of educational level used as a proxy of the human capital accumulated on the choice of the working labour force status in Cameroon. Using discrete-choice models on data draw from the second Employment and Informal Sector Survey carried out by the National Institute of Statistics, empirical findings reveal that educated young people are more disposed to make a decision to work as wage earned and the corresponding probability rises with the increasing of their educational levels. However, their willingness to become self-employed decline with the rising of their educational levels. Therefore, compared to the decision to work as a wage earned, becoming a self-employed does not appear as a human capital outcome. This paradoxical result for a low wage economy suggests a reform of the educational or training system starting at least in high school level with emphasizes in sectors with a high potential of self-employment and a setting-up of an apprenticeship plan.
The effective Ni(II) removal of red mud modified chitosan from aqueous solution
Authors: Luu Thi-Thuy , Nguyen Duy-Khoi , Nguyen Tu Thi Phuong , Ho Thien-Hoang , Dinh Van-Phuc , Kiet H.A. Tuan 
Published: October 2021

Abstract
To remove Ni(II) ions from an aqueous solution, researchers used red mud modified by chitosan (RM/CS) material as a new adsorbent. According to the findings, the surface area of red mud is nearly doubled after being treated with chitosan, from 68.6 m2/g to 105.7 m2/g. The effects of pH solution, contact time, and material dosage on the Ni(II) uptake were examined. In comparison with the pseudo-first-order and pseudo-second-order models, the intra-diffusion model was the most suitable kinetic model for the Ni(II) removal. Besides, the three-parameter Sips model was used to predict the Ni(II) adsorption of RM/CS from aqueous solution. Furthermore, the Langmuir maximum Ni(II) uptake capacity of this material was 31.66 mg/g at 323K, which was higher than red mud and several other natural materials. Notably, thermodynamic investigations demonstrated that Ni(II) adsorption on RM/CS is both exothermic and physic.
Clinical performance of the Roche Elecsys SARS-CoV-2 Antigen fully automated electrochemiluminescence immunoassay
Authors: Salvagno Gian Luca , Pighi Laura , De Nitto Simone , Lippi Giuseppe 
Published: October 2021

Abstract
Objective: We assessed the clinical performance of novel Roche Elecsys SARS-CoV-2 Antigen fully automated electrochemiluminescence immunoassay (ECLIA). Design and Methods: We tested 160 subjects, 110 (68.8%) with positive molecular test for SARS-CoV-2 infection in nasopharyngeal samples with Altona Diagnostics RealStar SARS-CoV-2 RT-PCR Kit and Roche Elecsys SARS-CoV-2 Antigen. The local imprecision was validated by analyzing three nasopharyngeal samples with different antigen concentration (1.84, 9.51 and 423.30 TCID50/mL) for 20 consecutive times (intra-assay imprecision) or for 10 consecutive working days (inter-assay imprecision).Results: The local intra- and inter-assay imprecision of Elecsys SARS-CoV-2 Antigen ECLIA was found to be comprised between 2.0-2.0% and 5.8-7.6%, yielding to a total imprecision of 6.2-7.8%. Highly significant correlation was found between Elecsys SARS-CoV-2 Antigen ECLIA and cycle threshold (Ct) values of SARS-CoV-2 S and E genes (both r=-0.91; p<0.001). The area under the curve (AUC), sensitivity and specificity of Elecsys SARS-CoV-2 Antigen ECLIA were 0.83, 0.43 and 1.00 in all samples, 0.99, 0.87 and 0.99 in those with both Ct values <30, as well as 1.00, 1.00 and 0.89 in samples with both Ct values <25. Conclusion: Roche Elecsys SARS-CoV-2 Antigen ECLIA may be a surrogate of molecular testing for identification of super-spreaders.
The evaluation of Melatonin and EGF interaction on breast cancer metastasis
Authors: Akbarzadeh Maryam 
Published: October 2021

Abstract
Background
Breast cancer is currently one of the most common types of cancer in women, and metastasis is the first cause of death in breast cancer patients. The epidermal growth factor (EGF) increases the invasion, growth, and migration of cancer cells. In the present study, melatonin, as a natural hormone, in EGF-induced tumor metastasis, was investigated.
Methods
First, MDA-MB-231 and MCF7 cells were cultured, and then the effects of melatonin on cell viability were determined by MTT assay. Transwell invasion assay was employed to identify the invasiveness of these breast cancer cell lines. Real-time RT-PCR then investigated the expression of MMP9 and MMP2. Cell proliferation was also determined under EGF and melatonin treatment using Ki67 assessment by flow cytometry.
Results
The rate of invasion and migration of EGF-treated cells increased in both groups, in which melatonin caused increased invasion by EGF in MCF7 cells. MMP9 and MMP2 expression increased significantly in both cell lines under EGF treatment, and melatonin increased these genes' expression in both cell lines (p <0.05). EGF increased the MMP9 and MMP2 gene expression, and melatonin increases EGF-induced expression(p <0.05). The EGF reduced the expression of the Ki67 protein in the MCF7 cell line, which was negatively affected by Melatonin and EGF. In contrast, along with Melatonin, EGF did not affect the proliferation of the MDA-MB-231 cell line.
Conclusions
Our results show that melatonin, as a natural compound, can increase the effects of EGF in the proliferation, migration, and invasion of cancer cells at low dosages.
KSHV transactivator-derived small peptide traps coactivators to attenuate MYC function and kills leukemia and lymphoma
Authors: Shimoda Michiko , Lyu Yuanzhi , Wang Kang-Hsin , Kumar Ashish , Miura Hiroki , Meckler Joshua F. , Davis Ryan R. , Chantarasrivong Chanikarn , Izumiya Chie , Tepper Clifford G. , Nakajima Ken-ichi , Tuscano Joseph , Barisone Gustavo , Izumiya Yoshihiro 
Published: October 2021

Abstract
In herpesvirus replicating cells, host cell gene transcription is frequently down-regulated because important transcriptional apparatuses are appropriated by viral transcription factors. Here, we identified a small peptide derived from the Kaposi's sarcoma-associated herpesvirus transactivator (K-Rta) sequence, which attenuates cellular c-MYC expression, reduces cell proliferation, and selectively kills cancer cell lines in both tissue culture and a xenograft tumor mouse model. Mechanistically, the peptide functions as a decoy to block the recruitment of coactivator complexes consisting of Nuclear receptor coactivator 2 (NCOA2), p300, and SWI/SNF proteins to the MYC promoter in primary effusion lymphoma cells. Thiol(SH)-linked alkylation for the metabolic sequencing of RNA (​SLAM seq) with target-transcriptional analyses further confirmed that the viral peptide directly attenuates MYC and MYC-target gene expression. This study thus provides a unique tool to control MYC activation, which may have significant potential as a therapeutic payload to treat MYC-dependent diseases such as cancers and autoimmune diseases.
Reconditioning the conditional [Recondicionando o condicional]
Authors: Miller David 
Published: October 2021

Many authors have hoped to understand the indicative conditional construction in everyday language by means of what are usually called conditional probabilities. Other authors have hoped to make sense of conditional probabilities in terms of the absolute probabilities of conditional statements. Although all such hopes were disappointed by the triviality theorems of Lewis (1976), there have been copious subsequent attempts both to rescue CCCP (the conditional construal of conditional probability) and to extend and to intensify the arguments against it. In this paper it will be shown that triviality is avoidable if the probability function is replaced by an alternative generalization of the deducibility relation, the measure of deductive dependence of Miller and Popper (1986). It will be suggested further that this alternative way of orchestrating conditionals is nicely in harmony with the test proposed in Ramsey (1931), and also with the idea that it is not the truth value of a conditionalstatement that is of primary concern but its assertability or acceptability.
Implementation Outcome Scales for Digital Mental Health (iOSDMH): Scale Development and Cross-sectional Study
Authors: Sasaki Natsu , Obikane Erika , Vedanthan Rajesh , Imamura Kotaro , Cuijpers Pim , Shimazu Taichi , Kamada Masamitsu , Kawakami Norito , Nishi Daisuke 
Published: October 2021

Background
Digital mental health interventions are being used more than ever for the prevention and treatment of psychological problems. Optimizing the implementation aspects of digital mental health is essential to deliver the program to populations in need, but there is a lack of validated implementation outcome measures for digital mental health interventions.

Objective
The primary aim of this study is to develop implementation outcome scales of digital mental health for different levels of stakeholders involved in the implementation process: users, providers, and managers or policy makers. The secondary aim is to validate the developed scale for users.

Methods
We developed English and Japanese versions of the implementation outcome scales for digital mental health (iOSDMH) based on the literature review and panel discussions with experts in implementation research and web-based psychotherapy. The study developed acceptability, appropriateness, feasibility, satisfaction, and harm as the outcome measures for users, providers, and managers or policy makers. We conducted evidence-based interventions via the internet using UTSMeD, a website for mental health information (N=200). Exploratory factor analysis (EFA) was conducted to assess the structural validity of the iOSDMH for users. Satisfaction, which consisted of a single item, was not included in the EFA.

Results
The iOSDMH was developed for users, providers, and managers or policy makers. The iOSDMH contains 19 items for users, 11 items for providers, and 14 items for managers or policy makers. Cronbach α coefficients indicated intermediate internal consistency for acceptability (α=.665) but high consistency for appropriateness (α=.776), feasibility (α=.832), and harm (α=.777) of the iOSDMH for users. EFA revealed 3-factor structures, indicating acceptability and appropriateness as close concepts. Despite the similarity between these 2 concepts, we inferred that acceptability and appropriateness should be used as different factors, following previous studies.

Conclusions
We developed iOSDMH for users, providers, and managers. Psychometric assessment of the scales for users demonstrated acceptable reliability and validity. Evaluating the components of digital mental health implementation is a major step forward in implementation science.
Chinese-Named Entity Recognition From Adverse Drug Event Records: Radical Embedding-Combined Dynamic Embedding–Based BERT in a Bidirectional Long Short-term Conditional Random Field (Bi-LSTM-CRF) Model
Authors: Wu Hong , Ji Jiatong , Tian Haimei , Chen Yao , Ge Weihong , Zhang Haixia , Yu Feng , Zou Jianjun , Nakamura Mitsuhiro , Liao Jun 
Published: October 2021

Background
With the increasing variety of drugs, the incidence of adverse drug events (ADEs) is increasing year by year. Massive numbers of ADEs are recorded in electronic medical records and adverse drug reaction (ADR) reports, which are important sources of potential ADR information. Meanwhile, it is essential to make latent ADR information automatically available for better postmarketing drug safety reevaluation and pharmacovigilance.

Objective
This study describes how to identify ADR-related information from Chinese ADE reports.

Methods
Our study established an efficient automated tool, named BBC-Radical. BBC-Radical is a model that consists of 3 components: Bidirectional Encoder Representations from Transformers (BERT), bidirectional long short-term memory (bi-LSTM), and conditional random field (CRF). The model identifies ADR-related information from Chinese ADR reports. Token features and radical features of Chinese characters were used to represent the common meaning of a group of words. BERT and Bi-LSTM-CRF were novel models that combined these features to conduct named entity recognition (NER) tasks in the free-text section of 24,890 ADR reports from the Jiangsu Province Adverse Drug Reaction Monitoring Center from 2010 to 2016. Moreover, the man-machine comparison experiment on the ADE records from Drum Tower Hospital was designed to compare the NER performance between the BBC-Radical model and a manual method.

Results
The NER model achieved relatively high performance, with a precision of 96.4%, recall of 96.0%, and F1 score of 96.2%. This indicates that the performance of the BBC-Radical model (precision 87.2%, recall 85.7%, and F1 score 86.4%) is much better than that of the manual method (precision 86.1%, recall 73.8%, and F1 score 79.5%) in the recognition task of each kind of entity.

Conclusions
The proposed model was competitive in extracting ADR-related information from ADE reports, and the results suggest that the application of our method to extract ADR-related information is of great significance in improving the quality of ADR reports and postmarketing drug safety evaluation.
Propiedades psicométricas del Cuestionario de Admiración y Rivalidad Narcisista (NARQ) en estudiantes universitarios de Venezuela
Authors: Chaustre Jota Daniel 
Published: October 2021

La investigación tuvo como objetivo analizar las propiedades psicométricas y la adecuación del Cuestionario de Admiración y Rivalidad Narcisista (NARQ) en una muestra no probabilística de estudiantes universitarios (N = 159; 74 % mujeres) de Caracas (Venezuela), pertenecientes a una universidad privada. A través del método bola de nieve se recabó la información por medio de la administración de un cuestionario en línea. Mediante el análisis factorial exploratorio se comprobó la solución factorial propuesta, también se hallaron relaciones significativas entre admiración y rivalidad con autoestima, extraversión, apertura y afabilidad, tal como se reporta en la literatura. Se concluye que el NARQ presenta evidencias de validez y confiabilidad, y por tanto se recomienda su uso en la población venezolana.
ANALISIS DE LOS RESULTADOS SABER PRO DEL PROGRAMA DE INGENIERÍA EN PRODUCCIÓN ACUÍCOLA AÑO 2016-2019
Authors: Cerón Benavides Sandra Milena , Romero-Ospina Manuel F. 
Published: October 2021

La presente investigación tuvo como objetivo definir las principales variables y su incidencia sobre el puntaje de las pruebas SABER PRO dentro del programa de Ingeniería en Producción Acuícola de la Universidad de Nariño en los últimos cuatro años (2016-2019), para ello se utilizaron 384 datos correspondientes a estudiantes de noveno semestre que presentaron las pruebas de estado.
Se determinó mediante diferentes técnicas multivariadas, que todas las variables inciden sobre las dos primeras componentes, con la necesidad de fortalecer Lectura crítica, Comunicación escrita y Competencia ciudadana, las cuales tienen mayor contribución sobre las tres últimas (>50) y por lo tanto impidieron que la variabilidad retenida en las primeras sea mayor al 60%.
Por otro lado, el incumpliendo del supuesto de homocedasticidad previo al Análisis de regresión múltiple no afecto la capacidad predictiva del modelo, la cual fue del 99%, el Análisis de regresión cuantílica y sus cinco modelos no presentaron diferencias significativas entre ellos, por lo que se concluye que todas las variables son indispensables para predecir el puntaje global, cada una con su grado de contribución, calidad de asociación y peso sobre las combinaciones lineales de las dos primeras componentes.
O beijo da mulher aranha (1981/1985) e as identificações e desidentificações pelas quais nos constituímos subjetivamente
Authors: Pontes Carlos Frederico Bustamante 
Published: October 2021

O trabalho em questão teve como eixo de análise o filme O beijo da mulher aranha (Kiss of the Spider Woman, Héctor Babenco,1985) e, como ponto de partida, o espetáculo teatral homônimo, de 1981, dirigido por Ivan de Albuquerque. A proposta do artigo foi analisar o filme sob o enfoque dos estudos de gênero e sexualidades e averiguar, criticamente, se a construção de cada um dos personagens centrais do filme, Molina e Valentin, bem como o relacionamento entre eles, apontam para identificações positivas e/ou negativas dos sujeitos LGBTQAI+ com a obra. Construídos em moldes de comportamento dissidentes e hegemônicos, concluiu-se que os dois personagens citados e o filme como um todo favorecem tanto para a discussão cada vez mais atual e necessária acerca do lugar do cinema nos processos de constituição subjetiva dos/as espectadores/as quanto para avaliarmos sua atuação sociocultural enquanto veículo de reafirmação e/ou de desconstrução dos modelos de sexo e gênero dominantes.
The effect of hormone replacement therapy on the survival of UK women: a retrospective cohort study 1984−2017
Authors: Akter Nurunnahar , Kulinskaya Elena , Steel Nicholas , Bakbergenuly Ilyas 
Published: October 2021

Objective: To estimate the effect of oestrogen-only and
combined hormone replacement therapy (HRT) on the hazards of overall and
age-specific all-cause mortality in healthy women aged 46 to 65 at first
prescription.
Design: Matched cohort study.
Setting: Electronic primary care records from The Health
Improvement Network (THIN) database, UK (1984−2017).
Population: 105,199 HRT users (cases) and 224,643 non-users
(controls) matched on age and general practice.
Methods: Weibull Double-Cox regression models adjusted for age
at first treatment, birth cohort, type 2 diabetes, hypertension and
hypertension treatment, coronary heart disease,
oophorectomy/hysterectomy, body mass index, smoking, and deprivation
status.
Main outcome measures: All-cause mortality.
Results: A total of 21,751 women died over an average of 13.5
years follow-up per participant, of whom 6,329 were users and 15,422
non-users. The adjusted hazard ratio (HR) of overall all-cause mortality
in combined HRT users was 0.91 (95%CI 0.88−0.94), and in oestrogen-only
users was 0.99 (0.93−1.07), compared to non-users. Age-specific adjusted
HRs for participants aged 46-50, 51-55, 56-60, and 61-65 years at first
treatment were 0.98 (0.92−1.04), 0.87 (0.82−0.92), 0.88 (0.82−0.93), and
0.92 (0.85−0.98), for combined HRT users compared to non-users, and 1.01
(0.84−1.21), 1.03 (0.89−1.18), 0.98 (0.86−1.12), and 0.93 (0.81−1.07)
for oestrogen-only users, respectively.
Conclusions: Combined HRT was associated with a 9% lower risk
of all-cause mortality and oestrogen-only formulation was not associated
with any significant changes.
Funding: IFoA.
Keywords: Hormone replacement therapy, menopause, mortality,
primary care records, THIN.
Tweetable abstract: Oestrogen-only HRT is not associated with
all-cause mortality and combined HRT reduces the risks.