bluuebunny/crossref_metadata_2025_split huggingface dataset:
Expression analysis and tissue localization of IgZ in the grouper Epinephelus coioides after Vibrio alginolyticus infection and vaccination
Authors: Fu Yao-Wu , Yao Zhan-Juan , Cui Miao , Zhang Qi-Zhong 
Published: April 2021

Abstract
Background
The orange-spotted grouper (Epinephelus coioides) is an important marine farmed fish in China. It is affected by the bacterial pathogen Vibrio alginolyticus, which causes high mortality and substantial economic losses.
Results
We studied the transcriptional changes of the IgZ gene in E. coioides following V. alginolyticus stimulation and investigated the distribution of IgZ in different tissues. The highest expression level of IgZ occurred in the head kidney. When fish were exposed to live and inactivated V. alginolyticus, the expression levels of IgZ in head kidney, spleen, intestine, gill, and blood cell were significantly upregulated. In an in situ hybridization study, IgZ mRNA-positive cells were detected in head kidney, spleen, and gill, but positive signals were not detected in liver and intestine. IgZ-labeled cells increased in the head kidney, spleen, and gill post-infection with V. alginolyticus for 21 days.
Conclusions
The present study provided additional evidence that IgZ was involved in mucosal immune responses against pathogens. These results help explain the role of IgZ in E. coioides defense against V. alginolyticus infection.
Impact of a health educational interventional program on reducing the head lice infestation among pupils in an elementary school of a sub-tropical region
Authors: Najjari Mohsen , Gorouhi Mohammad Amin , Zarrinfar Hossein , Farash Bibi Razieh Hosseini , Jamali Jamshid , Moghaddas Elham , Ebrahimipuor Mohammad 
Published: April 2021

Abstract
Background

Pediculosis is an important social challenge that can be caused by human head louse, Pediculus humanus capitis. This infestation is cosmopolitan, especially in countries with low hygiene and sanitation. Regular health education classes for students and their parents are required to reducing this infestation in schools and families. This study aimed to evaluate the impact of a health education intervention program on reducing of head lice among pupils of an elementary school.
Methods

In a case-control study, a total of 594 pupils, a girl elementary school, were screened for pediculosis (2015–2016). Interventions were applied in two steps, pediculosis cases eradication and training-oriented prevention program. Visual inspection was applied for initial diagnosis of infection. The suspected cases were confirmed by wood lamp examination and potassium hydroxide (KOH) microscopic wet-mount examination. An elementary school in the same area was selected as the control group, with no interference. The prevalence of contamination by the same method was obtained in the study group.
Results

At the beginning of study, the overall prevalence of pediculosis among pupils was 8.4% (49/594). The mean age in all pupils was 9.86 ± 1.83 years old and the most infestation was showed in fourth-grade students with 10 years old. Analysis of statistics demonstrated a significance difference between having infestation and the number of members in the families. The interventions led to a significant increase of parenting knowledge on prevention program of pediculosis (p-value < 0.001), and a decreased prevalence of pediculosis in pupils to 3% (8/594) (p-value < 0.05).
Conclusions

The prevalence of pediculosis was significantly reduced following the interventions in the school. The applied interventions may be implemented in other residual centers to get rid of this important infestation.
Geographic Disparities in Saskatchewan Prostate Cancer Incidence and its Association with Physician Density: Analysis Using Bayesian Models.
Authors: Andkhoie Mustafa , Szafron Michael 
Published: April 2021

Abstract
Background: Saskatchewan has one of the highest incidence of prostate cancer (PCa) in Canada. This study assesses if geographic factors in Saskatchewan, including location of where patients live and physician density are affecting the PCa incidence. First, the objective of this study is to estimate the PCa standardized incidence ratio (SIRs) in Saskatchewan stratified by PCa risk-level. Second, this study identifies clusters of higher than and lower than expected PCa SIRs in Saskatchewan. Lastly, this study identifies the association (if any) between family physician density and estimated PCa SIRs in Saskatchewan. Methods: First, using Global Moran’s I, Local Moran’s I, and the Kuldorff’s Spatial Scan Statistic, the study identifies clusters of PCa stratified by risk-levels. Then this study estimates the SIRs of PCa and its association with family physician density in Saskatchewan using the Besag, York, and Mollie (BYM) Bayesian method.Results: Higher than expected clusters of crude estimated SIR for metastatic PCa were identified in north-east Saskatchewan and lower than expected clusters were identified in south-east Saskatchewan. Areas in north-west Saskatchewan have lower than expected crude estimated SIRs for both intermediate-risk and low-risk PCa. Family physician density was negatively associated with SIRs of metastatic PCa (IRR: 0.935 [CrI: 0.880 to 0.998]) and SIRs of high-risk PCa (IRR: 0.927 [CrI: 0.880 to 0.975]). Conclusions: This study identifies the geographical disparities in risk-stratified PCa incidence in Saskatchewan. The study identifies areas with a lower family physician density have a higher-than-expected incidences of metastatic and high-risk PCa. Hence policies to increase the number of physicians should ensure an equitable geographic distribution of primary care physicians to support early detection of diseases, including PCa.
Decreased Prevalence of the Plasmodium Falciparum Pfcrt K76T and Pfmdr1 N86Y Mutations Post- Chloroquine Treatment Withdrawal in Katete District, Eastern Zambia
Authors: Mwenda Mulenga Chilumba , Sitali Lungowe , Ciubotariu Ilinca , Hawela Moonga B , Hamainza Busiku , Mharakurwa Sungano 
Published: April 2021

Abstract
Background: In 2002, Zambia withdrew chloroquine as first line treatment for Plasmodium falciparum malaria due to increased treatment failure and world-wide spread of chloroquine resistance. The artemisinin combination regimen artemether-lumefantrine replaced chloroquine as first choice malaria treatment. The present study determined the prevalence of chloroquine resistance molecular markers in the malaria parasite Pfcrt and Pfmdr1 genes in Eastern Zambia at nine and thirteen years after the removal of drug pressure.Methods: We assayed by polymerase chain reaction (PCR) and restriction fragment length polymorphisms (RFLP) the prevalence of the genetic mutations, K76T on the Pfcrt gene and N86Y on the Pfmdr1 gene in samples collected from Katete District during drug therapeutic efficacy assessments conducted in 2012 and 2016.Results: A total of 204 P. falciparum positive samples from 2012 and 2016 were further analysed for Pfcrt K76T and Pfmdr1 N86Y. 112 P. falciparum infected samples collected in 2012 were successfully amplified for Pfcrt and Pfmdr1, while 69 (65.7%) and 72 (68.6%) samples from 2016 were successfully amplified for Pfcrt and Pfmdr1. In 2012, the prevalence of Pfcrt 76K was 97.3%, 76T was 1.8%, and 0.8% had both 76K and 76T codons. The prevalence of Pfmdr1 86N was 97.9% and 86Y was 2.1%. In the 2016 samples, the prevalence of the respective parasite genotypes was 100% Pfcrt 76K and Pfmdr1 86N.Conclusion: This study shows that there was a complete recovery of chloroquine-sensitive parasites by 2016 in Katete District, thirteen years following the withdrawal of CQ. These findings add to the body of evidence for a fitness cost in chloroquine-resistant P. falciparum in Zambia and elsewhere. Further studies are recommended to explore the feasibility of integration of the former best antimalarial in combination therapy in the future.
Regional comparative analysis of factors that may delay child-birth for at least 10 months among women in Ghana after marriage
Authors: Iddrisu Abdul-Karim , Adams Mustapha , Odametey Akuye-Shika , Mensah Nana Kofi , Kwotua Paul A. 
Published: April 2021

Abstract
Introduction: Infertility or difficulty related to conception and childbirth is still a global health concern including Ghana. Infertility or child-birth associated difficulties after marriage can lead to divorce. However, risk factors associated with child-birth difficulties are often difficult to detect by both the Gynaecologist and the couple involved. This makes proposing solutions to issues related to child-birth usually unsuccessful.Methods: This study seeks to identify risk factors associated with child-birth among married women in Ghana. This was achieved by using a logistic regression model for the dichotomous birth length (birth within 10 months or after 10 months in marriage) variable, adjusting for risk factors of birth length. The data used for the study were obtained from the 2014 Ghana Demographic and Health Survey, consisting of 5,004 complete cases. Statistical analyses were carried out using STATA version 14.1, best-fitting model selected from candidate models using their respective Akaike Information Criterion or Bayesian Information Criterion and the predictive power of such model determined using the Receiver Operating Characteristic curve.Results: Respondents with Akan ethnic background are less likely to deliver their first child after 10 months of marriage, wives who reported that beating is justified if she goes out without her husband's notice are more likely to give birth to their first child after 10 months of marriage, wives who reported that beating is justified when she burns food are less likely to give birth to their first child after 10 months of marriage. A unit increase in the age of respondent at first sex at first cohabitation decreases the likelihood of giving birth to the first child after 10 months in marriage. Rich and middle-class wives are more likely to give birth after 10 months in marriage.Discussion and Conclusions: For wives to conceive within 10 months of marriage, wives and husbands should or are encouraged to have frequent sex, any negative social behavior or policies must be discouraged. Husbands should openly express their desire and love for their children since this increases the likelihood of wives desire to give birth. This leads to frequent sex, which then reduces conception time, and hence, child-birth within the shortest possible time.
Oridonin Improves the Therapeutic Effect of Lentinan on Lung Cancer
Authors: Gui Yun , Cheng Jing , Chen Zhiguo 
Published: April 2021

Abstract
Oridonin, a compound from Rabdosia rubescens, has been shown to have a potency for the improvement of the antitumor effect of lentinan (LNT). In this study, we tested the effect of oridonin, LNT, and the combination of them on a normal human fetal lung fibroblast cell line MRC-5 and non-small cell lung cancer cell line A549. Then we tested their effects on metastasis and survival with a lung cancer mice model. The effects of them on the mRNA and protein expression of several regulatory factors in A549 and lung tissue were determined by QPCR and western blotting. Results showed that the viability of MRC-5 and A549 were not affected by 0-20 µg/ml oridonin. 0-300 µg/ml LNT did not affect the viability of MRC-5, but 50-400 µg/ml LNT inhibited the viability of A549. 20 µg/ml oridonin and 100 or 300 µg/ml LNT were used in the subsequent study. The oridonin, LNT, or the combination of both had no effect on MRC-5 cell viability. The oridonin had no effect on A549 cell viability but LNT suppressed A549 cell viability, and oridonin promoted the suppression of LNT on A549 cells. In vivo study showed that oridonin alone had no effect on metastasis and survival but LNT decreased metastasis and survival in mice. Oridonin improved the suppression of LNT against metastasis and further improved the survival rate. In both A549 and lung tissues, LNT increased the mRNA and protein expression of caspase-3, caspase-8, caspase-9, Bax, p53, p21, and IκB-α, reduced mRNA and protein expressions of Bcl-2 and NF-κB. Oridonin enhanced all the effects of LNT on cells. Our study demonstrated that oridonin enhanced the antitumor effects of LNT and is conducive to the development of oridonin and LNT as a novel cancer drug regimen.
Gastrin-17 Combined With CEA, CA12-5 and CA19-9 Improves the Sensitivity For the Diagnosis of Gastric Cancer
Authors: Wang Zhen , Mo Tang-ming , Tian Lei , Chen Jun-qiang 
Published: April 2021

Abstract
Background: Previous studies reported the utility of serum tumor markers (such as CEA, CA12-5 and CA19-9) and gastrin-17 in the diagnosis of gastric cancer (GC). However, the value of these serum markers for diagnosing GC is still under debate.Methods: The level of CEA, CA12-5, CA19-9 and gastrin-17 was tested in 230 GC patients and 99 healthy people. The value of the four markers for diagnosing GC was analyzed.Results: The positive rate of Gastrin-17, CEA, CA199 and CA125 were much higher in GC group (22.61%, 22.61%, 20.00% and 8.26%, respectively) than that of healthy control group (5.05%, 2.02%, 1.01% and 2.02%, respectively). The sensitivity of Gastrin-17, CEA, CA125 and CA199 in the diagnosis of GC were 22.61%, 22.61%, 6.96% and 20.00%, respectively, and the corresponding specificity were 94.95%, 97.98%, 98.99% and 98.99%, respectively. By using the optimal cut-off value derived from the area under curve (AUC) of receiver operating characteristic curve, the AUC of gastin-17, CEA, CA125, CA199 increased to 0.72, 0.64, 0.61 and 0.65, respectively. After combining the four markers, the AUC increased to 0.79 (95% CI: 0.75-0.84), and the corresponding sensitivity and specificity were 65.22% (95% CI: 58.70% - 71.40%) and 84.85% (95% CI: 76.20% - 91.30%), respectively.Conclusion: CEA, CA12-5, CA19-9 and gastrin-17 were all valuable in the diagnosis of GC, and gastrin-17 had the best diagnostic value among the four markers. Gastrin-17 combined with CEA, CA12-5 and CA19-9 could improve the diagnostic value of GC significantly. Prospective, multi-center studies are needed to validate our findings.
Marital Status is an Independent Prognostic Factor in 7 Sites of Squamous Cell Carcinoma: A Propensity-Adjusted SEER Database Analysis
Authors: Xu Ji-li , Guo Yong 
Published: April 2021

Abstract
Background: Marital status has been proved as an independent prognostic factor in many cancer types. However, no detailed investigation of marital status on squamous cell carcinoma (SCC) has been evaluated. The aim of this essay is to explore the relationship between marital status and SCC in 7 tumor sites. Methods: All patients diagnosed with SCC were collected from the SEER database (1975-2016). We analyzed the survival of all included SCC patients in four marital status. We utilized propensity-score matching analysis to balance baseline characteristics between married and unmarried SCC patients in 7 tumor sites. The influence of marital status on overall survival (OS) in each site was performed by Cox regression analysis.Results: A total of 180009 SCC patients were involved in this study. After propensity-score matching, patients in the married group were 1:1 matched with patients in the unmarried group for each sites. Married group exhibited higher 5- year OS rate than unmarried group (27.3% vs 19.8%). More precisely, being divorced and widowed were observed to be related to have worse survival than single patients in most sites. Furthermore, patients with clinical stage IV were more common in the unmarried group which having a lower proportion of receiving treatment. Conclusions: This study indicated that marital status was a significant factor for OS of SCC in 7 tumor sites. Married patients always behaved more favorable than unmarried including single, divorced, and widowed patients.
Relationship Between Preoperative Neutrophil-Lymphocyte and Platelet-Lymphocyte Ratios in the Probable Diagnosis of Malignant or Borderline Ovarian Tumors
Authors: Yoon Tae Hui , Cho Eun Byeol , Seol Ki Ho , Ryu Jung Min , Choi Youn Seok 
Published: April 2021

Abstract
Background: The purpose of this study was to investigate whether neutrophil-lymphocyte ratio (NLR) and platelet-lymphocyte ratio (PLR) can be supplementary tools to differentiate benign, borderline, and malignant ovarian tumors.Methods: This retrospective study reviewed the postoperative histopathology in patients with ovarian tumors (220 benign, 59 borderline, and 228 malignant). White blood cell, platelet, neutrophil and lymphocyte counts, percentage of neutrophils and lymphocytes, calculated NLR and PLR were analyzed between groups using complete blood count tests performed before surgery. Results: The platelet count and PLR in borderline ovarian tumors tended to be statistically close to benign ovarian tumors, while the neutrophil and lymphocyte count, NLR tended to be statistically close to malignancy. The diagnostic cut-off value of NLR for differentiating between benign and borderline was 2.42, PLR for differentiating between borderline and malignancy was 140.96. When the NLR was 2.4 or higher, the odds ratio of borderline or malignant risk was 3.264. In the case of PLR, 140 or higher, the odds ratio of malignancy was 1.916. When both PLR and NLR were above each cut-off, the sensitivity of malignancy diagnosis was 51.5%, specificity was 77.0%. Conclusions: In the case of borderline ovarian tumors, the NLR was higher than benign and similarly tend to malignancy, but the PLR was lower than malignancy and similarly tend to benign. We suggest that the NLR and PLR can be used as a supplementary tool for diagnosing benign, borderline, and malignant ovarian tumors in addition to imaging diagnosis and tumor markers such as CA125.
Las necesidades educativas especiales y trastorno de espectro autista en la construcción del principio de interés superior del niño, niña y adolescente en el Derecho Chileno
Authors: Astudillo Meza Constanza 
Published: April 2021

El presente artículo desarrolla las necesidades especiales en el Derecho chileno como criterio para poder determinar el interés superior del niño, niña o adolescente en el contexto del derecho a la educación. El tema será abordado desde una perspectiva analítica y dogmática, de forma tal de proponer la construcción del nombrado interés superior considerando los requerimientos especiales de educación de los niños, niñas y adolescentes, particularmente en los casos del trastorno de espectro autista. Lo anterior, tomando en consideración la relevancia y ventajas que ofrece el principio ya mencionado, toda vez que permite adaptarlo a cada realidad y caso particular.
Características clínicas y patológicas de pacientes con tumores del sistema nervioso central en un centro en Suramérica desde 2010 hasta 2015
Authors: Jaramillo-Jiménez Esteban , Vargas-García Cristian , Rodríguez-Márquez Iader , Sandoval-Barrios Juliana , Gómez-Duque David , Barrientos-Montoya Hernán , Arango-Vélez Julián , Gómez-Escobar Tatiana , Massaro-Ceballos Mónica , Jiménez-Jaramillo Marta 
Published: April 2021

Objetivo: Caracterizar los pacientes diagnosticados con tumores del sistema nervioso central en el Instituto Neurológico de Colombia durante el periodo 2010-2015. Métodos: Estudio descriptivo retrospectivo. Para los tumores primarios se usó la clasificación de la Organización Mundial de la Salud y para los metastásicos se usó la Clasificación Internacional de Enfermedades en Oncología. Resultados: Se identificaron 288 pacientes, 194 de ellos con tumores primarios y 94 tumores metastásicos. No se lograron clasificar los tumores primarios en el 23,7 % de los casos y para los metastásicos no se obtuvo la clasificación morfológica (histológica) en el 35,1 % de los casos. Los hombres presentaron con mayor frecuencia tumores de comportamiento maligno tipo glioblastoma NEO (no especificado de otra manera) (14,9 %) y en las mujeres predominaron los tumores de comportamiento benigno tipo meningioma (23,2 %). En mayores de 65 años, tanto el glioblastoma NEO como el meningioma fueron más frecuentes en mujeres con 17,4 % y 28,3 %, respectivamente. Entre los pacientes con tumores metastásicos, de acuerdo con la clasificación topográfica, los tumores primarios más frecuentes se localizaron en pulmón (39,4 %) y mama (17 %). No se identificó el sitio primario de metástasis en el 11,7 % de los casos. La histología más comúnmente identificada fue el adenocarcinoma (14,9 %), seguido del carcinoma (8,5 %). Conclusiones: Para una vigilancia efectiva de la enfermedad es necesario realizar un monitoreo epidemiológico y clínico de tumores primarios y metastásicos, mediante el uso de registros institucionales de cáncer incluyendo datos topográficos, histológicos y moleculares, según disponibilidad.
Implantation of mechanical prosthesis in the pulmonary artery after radical correction of the common arterial trunk followed by xenopericardial conduit replacement: a case report
Authors: Barbukhatti K.O. , Belash S.A. , Shevchenko S.S. , Amari M.M. , Karakhalis N.B. , Shadrin A.K. , Porhanov V.A. 
Published: April 2021

Herein, we report the successful surgical treatment of the case of a 13-year-old patient with combined pulmonary artery stenosis, critical stenosis of the xenopericardial conduit and aortic valve insufficiency after two previous operations (radical correction of the common arterial trunk during the neonatal period and replacement of the right ventricular excretory tract conduit with branch plasty at three years of age). According to echocardiography, the peak pressure gradient across the conduit was 110 mmHg, along with aortic valve regurgitation of the third degree. According to computerised tomography angiography, there was narrowing of the right pulmonary artery up to 3 mm distally along with total calcification of the conduit and branches of the pulmonary artery with an intimate fit to the chest wall. The trunk of the pulmonary artery was replaced with a valve-containing conduit CARBOMEDICS СARBO-SEAL No.25 (LivaNova PLC, London, United Kingdom); right pulmonary artery plasty was performed with a xenopericardial patch; aortic valve was replaced with CARBOMEDICS СARBO-SEAL No.23 (LivaNova PLC, London, United Kingdom), a mechanical prosthesis, and supracoronary prosthetics were used for the ascending aorta. The duration of cardiopulmonary bypasswas 309 minutes, cross-clampwas 142 minutes and circulatory arrest was 49 minutes, with 26°C hypothermia. Extubation was performed 10 hours after surgery, and he spent 3 days in intensive care. The duration of hospitalisation was 26 days. He was examined after 6 months, and there were no complaints. The presented clinical case shows that implantation of a mechanical prosthesis is justified with repeated reoperations on the outflow tract of the right ventricle and the pulmonary trunk, and it may be the surgery of choice.Received 3 September 2020. Revised 1 October 2020. Accepted 5 October 2020.Funding: The study did not have sponsorship.Conflict of interest: Authors declare no conflict of interest.Author contributionsDrafting the article: S.A. Belash, M.M. AmariLiterature review: S.A. Belash, M.M. AmariIllustrations: S.S. Shevchenko, A.K. ShadrinCritical revision of the article: K.O. Barbukhatti, S.A. Belash, N.B. Karakhalis, V.A. PorkhanovFinal approval of the version to be published: K.O. Barbukhatti, S.A. Belash, S.S. Shevchenko, M.M. Amari, N.B. Karakhalis, A.K. Shadrin, V.A. Porkhanov