bluuebunny/crossref_metadata_2025_split huggingface dataset:
N6-methyladenosine-related lncRNAs as a biomarker for predicting the prognosis and immune microenvironment of clear cell renal cell carcinoma
Authors: Qiu Yuqin , Wang Xiaogang , Fan Zhenjia , Zhang Shanhui , Huang Jinchang , Jiang Xin 
Published: March 2021

Abstract
Background: Patients with advanced clear cell renal cell carcinoma (ccRCC) have a poor prognosis and lack effective prognostic biomarkers. This study uses bioinformatics analysis to identify N6-methyladenosine-related lncRNAs (m6A-related lncRNAs) as new prognostic biomarkers for ccRCC.Methods: Gene expression data and related clinical information of ccRCC patients were extracted from the Cancer Genome Atlas Database. m6A-related lncRNAs were obtained by co-expression analysis. Univariate Cox regression analysis was performed on these lncRNAs to find the prognostic-related m6A-related lncRNAs, and consensus clustering analysis was performed. The prognostic signature was screened by LASSO regression and a prognostic model was constructed. The predictive performance of the prognostic model was evaluated and validated by survival analysis and ROC curve analysis, etc. In addition, we also systematically analyzed the expression of immune checkpoints and immune cell infiltration in ccRCC patients.Results: First, 27 m6A-related lncRNAs associated with prognosis were identified, which were significantly differentially expressed between tumor and normal tissues. Consensus clustering analysis indicated that cluster 2 was associated with poor prognosis, low stromal score, high expression of PD-1, PD-L1, CTLA-4, LAG-3, TIM-3, TIGIT, and immunosuppressive cell infiltration. The signaling pathways related to tumor progression, drug resistance, and angiogenesis and biological processes related to protein methylation and phosphatidic acid metabolism are significantly enriched in cluster 2. Subsequently, LASSO regression analysis was used to construct a prognostic risk model based on 7 m6A-related lncRNAs signature, which can be used as an independent prognostic indicator. After a series of analyses, it was shown that this model had good sensitivity and specificity, can predict the prognosis of patients with different clinical stratifications and was associated with the progression of ccRCC. The expression levels of immune checkpoints were significantly increased in high-risk patients, and there was a certain correlation between the risk score and immune cell infiltration. Conclusions: In summary, we constructed and validated a risk model that can independently predict the prognosis of ccRCC patients and reflect the immune microenvironment based on m6A-related lncRNAs; the model is conducive to the screening of biomarkers of ccRCC prognosis and may have the potential to reflect the response of ccRCCs to immunotherapy.
Repeated Recurrences of Mucinous Prostate Carcinoma With Signet Ring Cells in the Urinary Bladder After Robotic-assisted Laparoscopic Radical Prostatectomy: a Case Report and Literatures Review
Authors: Bai Shengjiang , Xu Hang , Lei Haoran , Xiong Xingyu , Zheng Weitao , Wei Qiang , Yang Lu 
Published: March 2021

Abstract
Background: Mucinous prostate carcinoma (PCa) and signet-ring-cell PCa are two rare variants of prostate cancer, but the transformation of mucinous PCa into mucinous PCa with signet ring cells was extremely uncommon, little data of therapy was available. Case presentation: We reported such a case that a man was pathologically considered as mucinous PCa after receiving transurethral resection of the prostate, then he underwent robotic-assisted laparoscopic radical prostatectomy (RARP), the pathological diagnosis was mucinous PCa (pT2cN0M0), without any adjuvant therapies, the man had received regular follow-up with serum total prostate-specific antigen level <0.003 ng/ml. Nevertheless, he received transurethral resection of bladder tumor (TUR-BT) at 13 months, 22 months and 31 months after RARP, respectively, the first TUR-BT specimens was mucinous PCa, where the two other present mucinous PCa with signet ring cells. Conclusions: We reviewed the literatures and discussed the differential diagnosis, immunohistochemistry, prognosis of this rare carcinoma, emphasized the rarity of the histological change and demanded fundamental research clarifying the confusion of this change, which may be helpful to search for therapeutic targets and improve prognosis.
Quercetin and a plant substance, identified using a multi-copy number of DNA topoisomerase I in recombinant Pichia pastoris, inhibited MDA-MB-231 proliferation via different manners.
Authors: Gang Zhao , Pei Chao Sin , Fadzil Nur Adila , Kwok Lim Shern , Lan Chew Ai , Khoo Boon Yin 
Published: March 2021

Abstract
The study aimed to employ an in vivo strategy to construct a multi-copy number of human DNA topoisomerase I (hTopI) gene using pPIC3.5K vector in GS115 strain of Pichia. The yeast transformant (GS115-pPIC3.5K-hTopI) was then used to investigate the preliminary growth effect of a pure compound (quercetin) and a standardised subfraction of ethanolic red onion peel extract (F1). The underlying mechanisms of quercetin and F1 were then tested on MDA-MB-231 for the cell cycle profile and apoptosis by flow cytometry, and the mRNA expression of CYP genes by real-time PCR. His + yeast transformants (clones) with multi-copy inserts resistant to various concentrations of Geneticin were successfully selected in the study. The clones’ cell density was likely to be unaffected; only the total protein expression and enzyme activity were increased following the increased copy number of hTop1 in the host. The clone that showed the target enzyme's highest activity is said to respond specifically to growth inhibitors, whereby both quercetin and F1 were proven to be potential growth inhibitors as assessed by the MTT assay. In the process, quercetin reduced cell proliferation by inducing apoptosis and cell cycle arrest (S phase only), whereas F1 reduced cell proliferation by inducing cell cycle arrest only (S and G2 phases). Quercetin and F1 induced CYP1A1 and CYP1B1 (carcinogenicity) gene mRNA expression, but only F1 induced CYP2S1 (cytotoxicity) gene mRNA expression in the treated cells, suggesting that both quercetin and F1 inhibited the cell proliferation of MDA-MB-231 via different manners. The newly developed GS115-pPIC3.5K-hTopI can be used to select various potential substances for breast cancer treatment in the future.
Assessing Partial Triadic Analysis with MaximumEntropy Bootstrap: an application to BES Italianeducation indicators
Authors: Marco Caputi , Fenga Livio 
Published: March 2021

Abstract
In this paper we analyse a set of Italian equitable and sustainable well-being indicators, related to the education and training domain, considered at a regional level over different years. To this end, the Partial Triadic Analysis - a multiway approach which allows to properly deal with time-dependent data structures - will be used in conjunction with a bootstrap method to carry out the assessment of the outcomes obtained. The adopted resampling scheme - called Maximum Entropy Bootstrap - proved to be an appropriate analytical tool, given its ability to preserve the longitudinal data structure without incurring in the usual restrictive assumptions of more traditional bootstrap methods. To the best of the authors' knowledge, this is the first time a bootstrap method for longitudinal data has been employed in PTA.
Knowledge, Practices and Factors Affecting Exclusive Breastfeeding among Lactating Mothers with Babies aged&nbsp;6 months to 1 year attending&nbsp;Magomeni&nbsp;Reproductive and Child Health Clinic,&nbsp;Kinondoni, Dar&nbsp;es&nbsp;Salaam:&nbsp;Descriptive Cross Sectional Study
Authors: Bernard Anacletus , Musa Frank M , Peter Dedius E. 
Published: March 2021

Abstract
Background: Exclusive Breastfeeding defined as the infant has to receive only breast milk from his/her mother with the exception of other kinds of food for six months. Exclusive Breastfeeding helps to prevent diseases to infants and it reduces risk of ovarian cancer to mother.Methodology: Descriptive cross sectional study was conducted among 124 lactating mothers with infants aged 6 months to one year attending Magomeni RCH Clinic in Kinondoni, Dar es Salaam. Simple random sampling procedure was used to obtain 124 lactating mothers. Data was collected from mothers who agreed to participate and signed the consent form by the use of close ended Swahili version questionnaire on assessing knowledge, practices and factors affecting Exclusive Breastfeeding among Lactating Mothers with Babies aged 6 months to 1 year attending Magomeni Reproductive and Child Health Clinic in Kinondoni district, Dar es Salaam.Result: About 74 (59.7%) respondents had high knowledge on EBF and 92 (74.2%) respondents initiated breastfeeding within one hour after delivery while 77 (62.1%) practiced exclusive breastfeeding. Findings show that 64(51.6%) respondents denied that exclusive breastfeeding causes loss of shape of their breasts while 48 (38.7%) respondents accepted that exclusive breastfeeding causes loss of shape of their breasts. Only 12 (9.7%) respondents didn’t know if EBF causes loss of breasts shape or not. Moreover, 19 (15.3%) respondents had physical difficulties/problems like cracking of nipples, inadequate milk, while 105(84.7%) didn’t get any physical difficult. Therefore there is significant association between mothers belief of losing shapes of their breasts and EBF (p=0.05). Physical difficulties/problems to lactating mothers study is significantly associated with EBF (p = 0.000).Conclusion: The overall knowledge among the lactating mothers with babies aged six months to one year was high and had good exclusive breast feeding practice. Although majority of the respondents are knowledgeable about EBF, the results show a gap between knowledge and actual practice within six months which is a recommended duration for Exclusive Breast feeding.
The Effect of Resistance Training with Royal Jelly on Serotonin and Dopamine Receptors Genes Expression in the Hippocampus of a Rat Model of Alzheimer's Disease
Authors: Shenas Neda Pardeh , Salehi Omidreza , Hosseini Seyed Ali 
Published: March 2021

Abstract
Background

Studies have shown that exercises and the use of natural foods have favorable effects on Alzheimer's disease (AD). The purpose of this study was to investigate the effects of resistance training (RT) with royal jelly (RJ) on serotonin and dopamine receptors expression in hippocampal tissue of rats with AD.
Methods

In this experimental study, 56 rats with AD were randomly divided into (1) control, (2) RT, (3) RT + 100 mg/kg RJ, 4) RT + 200 mg/kg RJ, 5) 100 mg/kg RJ, 6) 200 mg/kg RJ2 and 7) sham. Also in order to review the effects of AD induction on serotonin and dopamine, 8 healthy rats selected as healthy control group. During 8 weeks the groups 3, 4, 5 and 6 received daily RJ with specific doses peritoneally and groups 2, 3 and 4 performed RT three sessions per week with 30–100 percentage of body weight. Independent sample t- test, One way ANOVA and two-way ANOVA was used to investigate the effect of RT, RJ and interaction of RT and RJ also Bonferroni’s post- hoc test was used to evaluate the difference between the doses of RJ using SPSS software (P ≤ 0.05).
Results

RT had a significant effect on the increase of dopamine (p = 0.001) and serotonin receptors (p = 0.001); RJ had a significant effect on the increase of dopamine receptor(p = 0.01) and serotonin (p = 0.001) also RT simultaneously with RJ consumption had a significant interactive effect on the increase of serotonin receptor (p = 0.001).
Conclusion

It appears that RT and RJ can enhance serotonergic and dopaminergic function in hippocampal tissue of rats with AD, however, the effects of RJ seems to be dose dependent.
Factors determining the compliance of patients receiving anti-VEGF therapy for macular diseases with long-term follow-up in real-life practice
Authors: Bobykin E. V. , Krokhalev V. Y. , Buslaev R. V. , Morozova O. V. 
Published: March 2021

Purpose: to identify statistically significant factors that determine adherence to long-term follow-up on the part of patients receiving anti-VEGF therapy for macular diseases.Material and methods. A retrospective analysis was performed on 247 patients (153 women, 94 men) aged 24 to 92 years treated with anti-VEGF for “wet” age-related macular degeneration (n = 164), diabetic macular edema (n = 18), macular edema due to retinal vein occlusion (n = 35) and myopic choroidal neovascularization (n = 30). Demographic data and treatment results were statistically analyzed with the STATISTICA 13.3 program (normal conditions for variables, non-parametric and parametric criteria were determined).Results. Follow-up duration was shown to depend on gender (women are more committed to long-term treatment, p < 0.05), diagnosis (the longest follow-up related to myopic choroidal neovascularization patients), initial and final visual acuity. The comparison of subgroups of patients with the longest (over 30 months, n = 56) and shortest (till 12 months, n = 92) follow-up showed that prolonged monitoring corresponds to higher values of the initial (p < 0.01) and final (p < 0.05) visual acuity, as well as a lower average number of injections over the entire follow-up (p < 0.001) and a younger age of patients (p = 0.02).Conclusion. The follow-up duration depends on gender and age, the intensity of treatment, as well as on the initial and final visual acuity at a significance level of ˂ 0.05. Also, the follow-up duration depends on the particular diagnosis. An indicator clearly reflecting the severity of the burden of treatment is proposed: the coefficient of therapy intensity which is inversely related to the patient’s adherence to therapy.
Electrophysiological markers of preclinical diagnosis of glaucomatous optic neuropathy
Authors: Kirillova M. O. , Zueva M. V. , Tsapenko I. V. , Zhuravleva A. N. 
Published: March 2021

Purpose: to evaluate the changes in electrophysiological indicators reflecting various aspects of the function of retinal ganglion cells (RGC) and their axons in the early diagnosis of glaucomatous optic neuropathy (GON).Material and methods. Two clinical groups, (1) 35 patients (60 eyes) aged 49 to 70 with suspected glaucoma and (2) 16 patients (30 eyes) aged 43–68 with initial primary open-angle glaucoma (POAG), and a comparison group of 38 relatively healthy subjects (45 eyes) aged 42–70 were tested for pattern-reversed visual evoked potentials (PVEP), transient and stationary pattern-ERGs (PERG) according to ISCEV, and photopic negative response (PhNR).Results. The P100 amplitudes in both clinical groups differed significantly from the norm in PVEP on small and large patterns. The elongation of peak latency (T) of P100 compared with norm was significant for the stimulus 1° in group 2. In both groups of patients, increased variability of the temporal parameters of PERG and PVEP for small patterns was found. In groups 1 and 2, a decrease in the amplitude of P50 and N95 peaks of transient PERG for all stimuli was revealed, which was the most significant for the 0.3° pattern. In group 1, the N95 peak was significantly delayed in PERG for large patterns. A statistically significant reduction in the steady-state PERG's amplitude was found in the groups of suspected glaucoma and initial POAG. The sharpest changes were found for small (0.8° and 0.3°) patterns. The elongation of T compared to the norm was most pronounced for PERG at 0.3°, but due to the high variability of temporary indicators within the group, it had no statistical significance. The amplitude of PhNR was significantly different from the norm in the ERG for a flash of 3.0 cd·sec/m2.Conclusion. In patients with suspected glaucoma, a decrease in the P100 VEP amplitude with the simultaneous elongation of T may be considered as a criteria for the plastic stage at the level of lateral geniculate nucleus. Markers of functional changes in RGCs are the decrease in the amplitude of PhNR in response to bright flash, and P50 and N95 of PERG for pattern size 0.3°. The results indicate a greater vulnerability of the parvocellular system to early events in the development of GON.
Stress in children and adolescent is a burning issue of today
Authors: Zakharova Irina N. , Ershova Irina B. , Tvorogova Tatyana M. , Glushko Yuliya V. 
Published: March 2021

Introduction.Stress and stress-induced disorders are not uncommon in pediatric practice. The range of causal stressors (information environment, gadgets, pandemic, armed conflicts, etc.) has expanded significantly these days. The article depicts the main clinical manifestations of stress reactions, pathogenetic mechanisms of their development, provides rational approaches to the therapy of elimination of stress manifestations and consequences in children and adolescents from a pediatric perspective.Objective:To study the influence of stress on the psychoemotional sphere and cognitive functions in children aged 7 to 9 years from the armed conflict zone in the Donbass.Materials and methods. 234 children of primary school age were included in the study, of whom 123 children had lived at the armed conflict zone in Donbass for a year. The psychoemotional state and cognitive functions status were determined by children’s tests using a scoring method to assess test results.Results and discussion.The tests with a scoring method to assess test results showed that 100% of children from the armed conflict zone had a chronic stress, 63% had a moderate to severe stress, a high frequency of various types of phobias, as well as impaired concentration and memory.Therapeutic approaches to the management of stress reactions directly depend on the cause and clinical manifestations of such reactions. The therapy strategy includes among other things general strengthening actions, psychotherapy, symptomatic and pathogenetic methods of treatment. In addition, both acute and chronic stress leads to intracellular magnesium deficiency and increased urinary magnesium wasting, as a large amount of catecholamines is released under stress conditions, which contributes to shifting magnesium out of cells. The magnesium deficiency results in increased permeability of cell membranes for calcium ions, which creates conditions for electrical instability and excessive excitability of cells, most significantly of neurons. This is reflected in the fact that the process of excitation prevails over inhibitory reactions, and stress reactions develop as the clinical manifestations. It has been established that an adequate balance of magnesium increases the adaptive capabilities in people. Its neurotropic effects made it possible to consider magnesium as an effective pathogenetic agent that can increase stress resistance, stress management, and activate the body’s adaptive reserves.Conclusion. The causal stressors are manifold, the paediatrician has to deal with stress reactions in children much more often than doctors of other specialties. Magnesium supplements currently form the basis of treatment and rehabilitation actions in children with stress.
Sentence matrimoniale pro nullitate erronée et secondes noces canoniques: un apax juridique à la suite de la faculté II du 11 février 2013
Authors: Danto Ludovic 
Published: March 2021

The subject of the article is the 2nd point of rescript of Benedict XVI of February 11, 2013: "Before the Roman Rota, it is not possible to apply for a new referral (nova causae propositio) after one of the parties has entered into a new canonical marriage", supplemented by 2nd point, 3 rescript of Francis of August 15, 2015 to add a final clause: "provided that the decision is clearly unfair".
In the first part of this study, the author discusses the canonical principle about a quasi-judged thing and the truth about the marriage bond, in order to conclude in the second part that both of the above-mentioned papal rescripts (the second one is more restrictive) limit the application of this principle in the cases indicated there, which prompts the question of the status of the second marriage defined by these norms.
In the summary Author assumes that if the norm (of the rescript) is expected to last, it cannot conclude that the new "canonical" spouses are in the new sacramental marriage, but rather it should be assumed that they are in a canonically recognized, however non-sacramental, relationship.
The Saudi Infection Control Guidance for Re-opening Dental Services in Governmental and Private Sectors During Coronavirus Disease-2019 Pandemic
Authors: AlAhdal Ali , Al-Huraishi Haila , Almalag Ahmad , Alrusayes Adel , Orfali Saud M 
Published: March 2021

Objective:
Novel newly discovered coronavirus, also known as severe acute respiratory syndrome coronavirus-2, is a recently emerging virus that has been rapidly spreading globally since December 2019. Due to the vicinity inoro-dental treatment and aerosol production, people inside the dental office are at high risk of being infected with severe acute respiratory syndrome coronavirus-2. This guideline aims to protect the dental health-care workers during their plans to re-open and increasingly continue their routine services until further notice from their governing body.

Methods:
A panel of experts in dentistry and infection prevention and control reviewed the local and global research and guidelines related to infection prevention and control during coronavirus disease-2019, along with the re-opening guidance provided by different entities.

Results:
Such a document might either be adopted or adapted to any regional and international organization that wishes to use a revised professional guideline in infection prevention and control dental services.

Conclusion:
A careful re-opening plan should be developed and implemented, including strict infection control measures before resuming the dental practice.
Compliance with Lockdown Regulations During the COVID-19 Pandemic in South Africa: Findings from an Online Survey
Authors: Dukhi Natisha , Mokhele Tholang , Parker Whadi-Ah , Ramlagan Shandir , Gaida Razia , Mabaso Musawenkosi , Sewpaul Ronel , Jooste Sean , Naidoo Inbarani , Parker Saahier , Moshabela Mosa , Zuma Khangelani , Reddy Priscilla 
Published: March 2021

Background:
Background: SARS-CoV-2 has resulted in the COVID-19 pandemic. Based on a nationally representative online survey conducted several weeks on the pandemic, this paper explores how South Africans responded to the compliance regulations laid down by the national government and factors associated with individuals’ confidence in their community adhering to lockdown regulations.

Methods:
The study was conducted using a closed-ended questionnaire on a data-free online platform. Additionally, a telephonic survey was included to accommodate individuals who do not have access to smart-phones. The study population consisted of respondents who were 18 years and older and living in South Africa (n=19 933). Data were benchmarked to the 2019 midyear population estimates. Descriptive statistics and bivariate logistic regression are presented.

Results:
Over a quarter (26.1%) of respondents reported that they had not left home, indicating compliance with the COVID-19 control regulations, and 55.3% who did leave their homes did so to purchase essential items. A small proportion (1.2%) reported that they had visited friends. People, classified as coloured, those who were more literate (those with secondary, matric and tertiary education status), those residing in disadvantaged areas (informal settlements, townships, rural areas and farms), and those who perceived their risk of contracting COVID-19 as moderate and high, reported not being confident of their community adhering to lockdown.

Conclusion:
Communication strategies must be employed to ensure that important information regarding the pandemic be conveyed in the most important languages and be dispatched via various communication channels to reach as many people as possible.