bluuebunny/crossref_metadata_2025_split huggingface dataset:
A modular microfluidic bioreactor to investigate plant cell–cell interactions
Authors: Finkbeiner T. , Manz C. , Raorane M. L. , Metzger C. , Schmidt-Speicher L. , Shen N. , Ahrens R. , Maisch J. , Nick P. , Guber A. E. 
Published: May 2021

AbstractPlants produce a wide variety of secondary metabolites, which often are of interest to pharmaceutical and nutraceutical industry. Plant-cell cultures allow producing these metabolites in a standardised manner, independently from various biotic and abiotic factors difficult to control during conventional cultivation. However, plant-cell fermentation proves to be very difficult, since these chemically complex compounds often result from the interaction of different biosynthetic pathways operating in different cell types. To simulate such interactions in cultured cells is a challenge. Here, we present a microfluidic bioreactor for plant-cell cultivation to mimic the cell–cell interactions occurring in real plant tissues. In a modular set-up of several microfluidic bioreactors, different cell types can connect through a flow that transports signals or metabolites from module to module. The fabrication of the chip includes hot embossing of a polycarbonate housing and subsequent integration of a porous membrane and in-plane tube fittings in a two-step ultrasonic welding process. The resulting microfluidic chip is biocompatible and transparent. Simulation of mass transfer for the nutrient sucrose predicts a sufficient nutrient supply through the membrane. We demonstrate the potential of this chip for plant cell biology in three proof-of-concept applications. First, we use the chip to show that tobacco BY-2 cells in suspension divide depending on a “quorum-sensing factor” secreted by proliferating cells. Second, we show that a combination of two Catharanthus roseus cell strains with complementary metabolic potency allows obtaining vindoline, a precursor of the anti-tumour compound vincristine. Third, we extend the approach to operationalise secretion of phytotoxins by the fungus Neofusicoccum parvum as a step towards systems to screen for interorganismal chemical signalling.
Confocal microscopy reveals alterations of thylakoids in Limnospira fusiformis during prophage induction
Authors: Zekri Maryam Alsadat , Schagerl Michael , Schweichhart Johannes , Lang Ingeborg 
Published: May 2021

AbstractThe alkaliphilic cyanobacteriumLimnospira fusiformisis an integral part in food webs of tropical soda lakes. Recently, sudden breakdowns ofLimnospirasp. blooms in their natural environment have been linked to cyanophage infections. We studied ultrastructural details and prophage components in the laboratory by means of confocal laser scanning microscopy (CLSM) and transmission electron microscopy (TEM). For a comparison at the subcellular level, we included transmission electron microscopy (TEM) material of infected cells collected during a field survey. Compared to TEM, CLSM has the advantage to rapidly providing results for whole, intact cells. Moreover, many cells can be studied at once. We chemically induced lysogenic cyanophages by means of mitomycin C (MMC) treatments and studied the ultrastructural alterations of host cells. In parallel, the number of cyanophages was obtained by flow cytometry. After treatment of the culture with MMC, flow cytometry showed a strong increase in viral counts, i.e., prophage induction. CLSM reflected the re-organization ofL. fusiformiswith remarkable alterations of thylakoid arrangements after prophage induction. Our study provides a first step towards 3D visualization of ultrastructure of cyanobacteria and showed the high potential of CLSM to investigate viral-mediated modifications in these groups.
Recreational Drug Use in People Living with HIV in Spain: Factors Associated with Drug Use and the Impact on Clinical Outcomes
Authors: Castro-Granell Vanessa , Garin Noé , Jaén Ángeles , Casado José Luis , Leal Lorna , Cenoz Santiago , Fuster-RuizdeApodaca María José 
Published: May 2021

AbstractWe analysed the impact of recreational drug use (RDU) on different outcomes in people living with HIV (PLHIV). A multicentre retrospective cohort study was performed with two cohorts of PLHIV included: people using recreational drugs (PURD) vs. people not using recreational drugs (PNURD). Overall, 275 PLHIV were included. RDU was associated with men having sex with men (OR 4.14, 95% CI [1.14, 5.19]), previous sexually transmitted infections (OR 4.00, 95% CI [1.97, 8.13]), and current smoking (OR 2.74, 95% CI [1.44, 5.19]). While the CD4/CD8 ratio increased amongst PNURD during the follow-up year, it decreased amongst PURD (p = 0.050). PURD presented lower scores of self-reported and multi-interval antiretroviral adherence (p = 0.017, and p = 0.006, respectively), emotional well-being (p < 0.0001), and regular follow-up (p = 0.059), but paid more visits to the emergency unit (p = 0.046). RDU worsens clinical, immunological, and mental health outcomes amongst PLHIV.
Volatility transmission and spillover dynamics across financial markets: the role of geopolitical risk
Authors: Elsayed Ahmed H. , Helmi Mohamad Husam 
Published: May 2021

AbstractThis paper examines the effect of geopolitical risk (GPR) on return and volatility dynamics in Middle East and North African (MENA) countries by using an ADCC-GARCH model and a spillover approach. Unlike previous studies, we include the GPR index to capture risk associated with wars, terrorist acts, and political tensions. Moreover, we test for both static and dynamic analysis using a rolling window. In brief, the findings highlight that GPR does not contribute to the return spillovers among MENA financial markets. However, the dynamic analysis provides evidence of the high level of responsiveness of the total spillover index to major political events (e.g., the Arab Spring uprising and political tension between Qatar and other Gulf Cooperation Council countries). More interestingly, Qatar, Kingdom of Saudi Arabia, and the United Arab Emirates are identified as the main transmitters of return spillovers to the rest of the MENA markets. Overall, our results are essential in understanding the impact of the GPR on return spillover among MENA countries, and are of particular importance to policymakers, market regulators, portfolio managers and investors.
Male and female breast cancer: the two faces of the same genetic susceptibility coin
Authors: Silva Susana Nunes , Gomes Bruno Costa , André Saudade , Félix Ana , Rodrigues António Sebastião , Rueff José 
Published: May 2021

Abstract
Background
Breast cancer (BC) is the most common cancer in women. In contrast, male BC is about 100 times less common than in women, being considered a rare disease. Male BC may be a distinctive subtype of BC and available data seems to indicate that male BC has a higher dependence on genetic variants than female BC. Nevertheless, the same prognostic and predictive markers are used to determine optimal management strategies for both male and female BC. Several studies have assessed the role of genetic polymorphisms (SNPs) in DNA repair genes in female BC susceptibility. However, data on male BC is scarce. Thus, the current study aimed to assess the role of SNPs in XRCC1, MUTYH and TP53 genes in a male cohort of BC, and, in addition, compare the male data with matched results previously genotyped in female BC patients.

Methods
The male BC cohort was genotyped through Real-Time PCR using TaqMan Assays for several SNPs previously analysed in Portuguese female BC patients.

Results
The results obtained indicate significant differences in BC susceptibility between males and females for the XRCC1 rs1799782, MUTYH rs3219489 and TP53 rs1042522 and rs8064946 variants.

Conclusions
In males, XRCC1 and TP53 variants, when in heterozygosity, seem to be related with lower susceptibility for BC, contrasting with higher susceptibility for a MUTYH variant in females. These findings may help to explain the difference in incidence of BC between the two sexes.
The Contribution of Professional Youth Work to the Personal Development and Social Participation of Socially Vulnerable Youngsters: A Dutch Longitudinal Cohort Study
Authors: Sonneveld Jolanda , Metz Judith , Manders Willeke , Schalk René , Van Regenmortel Tine 
Published: May 2021

AbstractThis article focuses on how length of participation in professional youth work is associated with five outcome variables: prosocial skills, self-mastery, social network, civic participation (volunteering and organizing activities) and finding support from social care services. The study was designed as a longitudinal cohort study of four waves during a 16-month period, gathering the data of 1597 youngsters aged 10–24 who participated in Dutch professional youth work. The results show that, on average, youngsters who participated longer in youth work scored significantly higher on the outcome variables. Respondents did not show individual improvements on outcome variables over the period, but the results demonstrate a cautious positive trend over time in volunteering. Referring youngsters (33%) by youth workers to care services could prevent an exacerbation of existing problems. The results provide knowledge that legitimizes the role of professional youth workers and which can be used by them to improve the support of socially vulnerable youngsters in their personal development and social participation.
Guidelines for Using Simulation for Online Teaching and Learning of Clinical Social Work Practice in the Time of COVID
Authors: Bay Uschi , Maghidman Marcelo , Waugh Jacinta , Shlonsky Aron 
Published: May 2021

AbstractDue to COVID 19, Monash University’s Social Work Department moved all clinical practice skills teaching in the Master of Social Work (graduate entry level) fully online using synchronous audio-visual conferencing platform Zoom for the first time from March to June 2020. The innovations associated with this move included the development of clinical practice laboratories (CPLs) to prepare 154 students for a modified version of an Objective Structured Clinical Examinations (OSCE) and their first field education practicum. The use of simulated clients to facilitate experiential learning of active listening skills, rapport-building and empathic communication in this mode of delivery is described in detail to encourage overcoming previous issues in teaching clinical practice skills to students located at a distance from campus.
Extending greedy feature selection algorithms to multiple solutions
Authors: Borboudakis Giorgos , Tsamardinos Ioannis 
Published: May 2021

AbstractMost feature selection methods identify only a single solution. This is acceptable for predictive purposes, but is not sufficient for knowledge discovery if multiple solutions exist. We propose a strategy to extend a class of greedy methods to efficiently identify multiple solutions, and show under which conditions it identifies all solutions. We also introduce a taxonomy of features that takes the existence of multiple solutions into account. Furthermore, we explore different definitions of statistical equivalence of solutions, as well as methods for testing equivalence. A novel algorithm for compactly representing and visualizing multiple solutions is also introduced. In experiments we show that (a) the proposed algorithm is significantly more computationally efficient than the TIE* algorithm, the only alternative approach with similar theoretical guarantees, while identifying similar solutions to it, and (b) that the identified solutions have similar predictive performance.
Comparing virtual vs real faces expressing emotions in children with autism: An eye-tracking study
Authors: Pino Maria Chiara , Vagnetti Roberto , Valenti Marco , Mazza Monica 
Published: May 2021

AbstractDifficulties in processing emotional facial expressions is considered a central characteristic of children with autism spectrum condition (ASC). In addition, there is a growing interest in the use of virtual avatars capable of expressing emotions as an intervention aimed at improving the social skills of these individuals. One potential use of avatars is that they could enhance facial recognition and guide attention. However, this aspect needs further investigation. The aim of our study is to assess differences in eye gaze processes in children with ASC when they see avatar faces expressing emotions compared to real faces. Eye-tracking methodology was used to compare the performance of children with ASC between avatar and real faces. A repeated-measures general linear model was adopted to understand which characteristics of the stimuli could influence the stimuli’s fixation times. Survival analysis was performed to understand differences in exploration behaviour between avatar and real faces. Differences between emotion recognition accuracy and the number of fixations were evaluated through a paired t-test. Our results confirm that children with autism have higher capacities to process and recognize emotions when these are presented by avatar faces. Children with autism are more attracted to the mouth or the eyes depending on the stimulus type (avatar or real) and the emotion expressed by the stimulus. Also, they are more attracted to avatar faces expressing negative emotions (anger and sadness), and to real faces expressing surprise. Differences were not found regarding happiness. Finally, they show a higher degree of exploration of avatar faces. All these elements, such as interest in the avatar and reduced attention to the eyes, can offer important elements in planning an efficient intervention.
Smartphone bans and workplace performance
Authors: Chadi Adrian , Mechtel Mario , Mertins Vanessa 
Published: May 2021

AbstractThis paper constitutes the first economic investigation into the potential detrimental role of smartphones in the workplace based on a field experiment. We exploit the conduct of a nationwide telephone survey, for which interviewers were recruited to work individually and in single offices for half a day. This setting allows to randomly impose bans on the use of interviewers’ personal smartphones during worktime while ruling out information spillovers between treatment conditions. Although the ban was not enforceable, we observe substantial effort increases from banning smartphones in the routine task of calling households, without negative implications linked to perceived employer distrust. Analyzing the number of conducted interviews per interviewer suggests that higher efforts do not necessarily translate into economic benefits for the employer. In our broad discussion of smartphone bans and their potential impact on workplace performance, we consider further outcomes of economic relevance based on data from employee surveys and administrative phone records. Finally, we complement the findings of our field experiment with evidence from a survey experiment and a survey among managers.
Expanding the FDS Simulation Capabilities to Fire Tunnel Scenarios Through a Novel Multi-scale Model
Authors: Verda Vittorio , Borchiellini Romano , Cosentino Sara , Guelpa Elisa , Tuni Jesus Mejias 
Published: May 2021

AbstractComputational Fluid Dynamics (CFD) is widely used to simulate tunnels and partially substitute on-site tests. As technology advances, new application opportunities appear; some examples are the optimal operation of ventilation and emergency systems, risk assessment of tunnels and training of the operators. Even when the computational capacity of computers has grown, CFD is still constrained by the large amount of computational resources needed in long tunnels. This introduces a need for methods able to reduce the amount of time required for simulations. To face this need, a novel 1D–3D multiscale model is presented in this paper. The model incorporates the code Whitesmoke into FDS (Fire Dynamics Simulator) through a direct coupling. Whitesmoke manages the fluid dynamics, temperature and concentration of species in the 1D portion, while FDS calculates these fields in the portion where fire occurs. Using this multiscale model, the computation time for long tunnels is reduced, proportionally to the 1D length in the domain. Also, additional simulation capabilities particularly useful for tunnel analysis are obtained. Some new characteristics are pressure boundary conditions can be easily imposed at the tunnel portals or at the ventilation shafts; the characteristic curves of the fans/jet-fans can be included, also considering the degradation effects due to smoke propagation; the piston effect can be properly considered. Our research verifies most of its capabilities, also clarifying its limitations and the criteria used to set the domain for the analysis. As a final step, the model is tested in a tunnel with a cross section of 4.8 m and 600 m of length with a 2 MW fire, comparing its performance with a full 3D FDS simulation. The difference in temperature and velocity is minimal for most of the domain, making It a good way to optimize resource usage in large simulations. Furthermore, the multiscale manages to reduce the computational time of more than a 50%.
Health-Related Quality of Life in Pediatric Patients with Syndromic Autism and their Caregivers
Authors: Bolbocean Corneliu , Andújar Fabiola N. , McCormack Maria , Suter Bernhard , Holder J. Lloyd 
Published: May 2021

AbstractChildren with autism have a significantly lower quality of life compared with their neurotypical peers. While multiple studies have quantified the impact of autism on health-related quality of life (HRQoL) through standardized surveys such as the PedsQL, none have specifically investigated the impact of syndromic autism. Here we evaluate HRQoL in children diagnosed with three genetic disorders that strongly predispose to syndromic autism: Phelan-McDermid syndrome (PMD), Rett syndrome (RTT), and SYNGAP1-related intellectual disability (SYNGAP1-ID). We find the most severely impacted dimension is physical functioning. Strikingly, syndromic autism results in worse quality of life than other chronic disorders including idiopathic autism. This study demonstrates the utility of caregiver surveys in prioritizing phenotypes, which may be targeted as clinical endpoints for genetically defined ASDs.
Do museums foster innovation through engagement with the cultural and creative industries?
Authors: Dalle Nogare Chiara , Murzyn-Kupisz Monika 
Published: May 2021

AbstractThe recent narrative on museums as catalysts of innovation and growth considers their relations with other cultural and creative industries (CCIs) to be very important. We argue that most relations museums establish with CCI firms and institutions are unlikely to produce strong positive externalities that make the latter more innovative. To prove this claim, we propose a conceptual framework qualifying project-based and supply chain relations between museums and CCIs as either strong, moderate, or weak links, according to their potential in terms of knowledge spillovers from museums to CCIs. We apply this taxonomy to data collected from 261 Polish museums. Our findings indicate that strong links are outnumbered by moderate and weak ones. We then suggest that the traditional missions of museums, in particular education and conservation, need to be more thoroughly assessed in terms of their direct and indirect contributions in order to fully capture the impact of museums on innovation in the wider economy.
A pragmatic approach to adjusting early instrumental local magnitudes for seismic hazard assessments in Australia
Authors: Allen Trevor I. 
Published: May 2021

AbstractPrior to the development of Australian-specific magnitude formulae, the 1935 magnitude correction factors by Charles Richter—originally developed for southern California—were almost exclusively used to calculate earthquake magnitudes throughout Australia prior to the 1990s. Due to the difference in ground-motion attenuation between southern California and much of the Australian continent, many earthquake magnitudes from the early instrumental era are likely to have been overestimated in the Australian earthquake catalogue. A method is developed that adjusts local magnitudes (ML) using the difference between the original (inappropriate) magnitude formulae (or look-up tables) and the Australian-specific formulae at a distance determined by the nearest recording station likely to have recorded the earthquake. Nationally, these adjustments have reduced the number of earthquakes ofML≥ 4.5 in the early instrumental catalogue by approximately 25% since 1900, while the number ofML≥ 5.0 earthquakes has reduced by approximately 32% over the same time period. The reduction in the number of moderate-to-large-magnitude earthquakes over the instrumental period yields long-term earthquake rates that are more consistent with present-day rates, since the development of Australian-specific magnitude formulae (approximately 1990). The adjustment of early instrumental magnitudes to obtain consistently derived earthquake catalogue is important for seismic hazard assessments.
Miquel dynamics, Clifford lattices and the Dimer model
Authors: Affolter Niklas C. 
Published: May 2021

AbstractMiquel dynamics was introduced by Ramassamy as a discrete time evolution of square grid circle patterns on the torus. In each time step every second circle in the pattern is replaced with a new one by employing Miquel’s six circle theorem. Inspired by this dynamics we consider the local Miquel move, which changes the combinatorics and geometry of a circle pattern. We prove that the circle centers under Miquel dynamics are Clifford lattices, an integrable system considered by Konopelchenko and Schief. Clifford lattices have the combinatorics of an octahedral lattice, and every octahedron contains six intersection points of Clifford’s four circle configuration. The Clifford move replaces one of these circle intersection points with the opposite one. We establish a new connection between circle patterns and the dimer model: If the distances between circle centers are interpreted as edge weights, the Miquel move preserves probabilities in the sense of urban renewal.
Transient vibrations of a fractional Zener viscoelastic cantilever beam with a tip mass
Authors: Freundlich Jan 
Published: May 2021

AbstractThe presented work concerns the kinematically excited transient vibrations of a cantilever beam with a mass element fixed to its free end. The Euler–Bernoulli beam theory and the fractional Zener model of the beam material are assumed. A fractional Caputo derivative is used to formulate a viscoelastic material law. A characteristic equation, modal frequencies, eigenfunction and orthogonality conditions are achieved for the beam considered. The equations of motion of the system are solved numerically. A numerical solution of a multi-term fractional differential equation is obtained by means of a conversion to a mixed system of ordinary and fractional differential equations, each of the order of$$0 < \gamma \le 1$$0<γ≤1. The transient time histories of the beam vibrations during the passage through resonance are calculated. A comparison between the beam responses obtained with a fractional and an integer viscoelastic material model is presented. The calculations performed reveal that use of the fractional damping affects on the time histories of the system. The calculated beam responses show that for some values of the order of the fractional derivative$$\gamma$$γ, the amplitudes occurring in the area of the second resonance are greater than those obtained in the area of the first resonance, which does not occur in the case of the integer order of the fractional derivative. Moreover, an evaluation is made of the difference between the results obtained for the calculations using the fractional Zener model and the fractional Kelvin model. It is shown that for some physical beam parameters, the calculation results obtained using both models are virtually the same for both models, which means that the the simpler, fractional Kelvin–Voigt material can be used instead of the fractional Zener material model. This simplifies the solution and decreases the time needed to make the numerical calculations.