bluuebunny/crossref_metadata_2025_split huggingface dataset:
Pyogenic Liver Abscesses Patients With Diabetes Mellitus Induced by Klebsiella Pneumoniae Are More Prone to Organ Dysfunction
Authors: Xue Mingming , Zeng Xiangpeng , Xu Feixiang , Shao Mian , Yang Yilin , Tao Zhengang , Yu Zhigao , Yin Jun , Gu Guorong , Song Zhenju , Tong Chaoyang , Yao Chenling , Xue Mingming 
Published: November 2021

Abstract
Background Diabetes mellitus (DM) has become a common risk factor for pyogenic liver abscess (PLA) patients, Klebsiella pneumoniae is a primary pathogen of PLA (KPLA). The purpose of this study was to evaluate the clinical and microbiological characteristics in PLA patients with or without DM induced by Klebsiella pneumoniae or not.Methods The clinical data of the total 557 PLA patients were collected in Zhongshan Hospital from January 2015 to December 2020. The liver abscess were confirmed using abdominal ultrasound (US)，computerized tomography (CT), and/or magnetic resonance imaging (MRI). The 557 patients were divided into two groups, PLA with DM and PLA without DM. In the group of PLA with DM, the patients were further separated into KPLA with DM and non-KPLA with DM.Results The total of 557 patients with PLA were analyzed, 225 (40.40%) patients comorbided DM. Among PLA patients with DM, there is a higher proportion of patients with hypertension (42.22%) and fatty liver diseases (38.67%), the most common clinical manifestation is frail and fatigue (18.22%), and more likely to progress to sepsis and metastatic infections such as periorbital infection. The PLA patients with DM have higher inflammatory markers (WBC, N%, CRP, ESR and PCT). There was significant difference between the group of PLA with DM and PLA without DM in the percentage of neutrophils, liver enzymes, albumin, glucose metabolism (blood glucose, glycated hemoglobin and glycated albumin), lipid metabolism (triglycerides, low density lipoprotein, high density lipoprotein), blood sodium and chlorine, blood urea nitrogen and higher heart markers ( proBNP and CK-MB). In KPLA patients with DM compared with non-KPLA with DM, inflammatory markers (WBC, N%, CRP, ESR and PCT), liver function index (TB, CB, ALT, AST, ALP and r-GT) and cardiac markers (cTnT and proBNP) are more higher. The level of albumin, serum sodium and ferritin are more lower between the KPLA patients with DM and non-KPLA with DM. Detection of liver abscess by high-throughput sequencing is more sensitive and accurate. There was no in-hospital mortality.Conclusions The study found that in the PLA patients with diabetic DM, Klebsiella pneumoniae infection is the most common, which is more prone to organ dysfunction and electrolyte disorder. High-throughput sequencing can help early diagnosis and accurate treatment of PLA patients.
Occurrence and Anti-microbial Susceptibility Pattern of Extended Spectrum Beta‑Lactamase Producing Enterobacteriaceae in Waste Water Released from Governmental Hospital of Addis Ababa, Ethiopia
Authors: Kebede Alehegn Amare 
Published: November 2021

Abstract
Background

Worldwide, come out and dissemination of Extended-spectrum beta-lactamases (ESBLs) producing Enterobacteriaceae has been warning the efficacy of antibiotics to treat an infection. Hospital wastewaters were a reservoir of such kind of resistant bacteria. Currently, the predominant antibiotics used for the treatment of hospitalized patients infected by Gram negative bacteria are the 𝛽-lactam antibiotics. So it is an important source to investigate the magnitude of ESBLs producing bacteria and their antimicrobial susceptibility pattern. The aim of this study was to determine the occurrence of ESBLs producing Enterobacteriaceae (ESBLs-pE) and their antibiotic susceptibility pattern in wastewater released from five governmental hospitals in Addis Ababa, Ethiopia.
Method

A cross-sectional study was carried out from April 1 to May 31, 2020. A total of 100 wastewaters were collected from five governmental hospitals in Addis Ababa using a grap-sampling technique. All Enterobacteriaceae were screened for ESBLs production using cefotaxime and ceftazidime as per 29th CLSI guideline. Each screen positive for ESBLs production was confirmed by the combination disk method (CDT) and their antibiotic susceptibility pattern were done using the Kirby-Bauer disk diffusion method on Muller Hinton agar (MHA). Data were entered and summarized using SPSS version 20 software.
Results

Of all Enterobacteriaceae, 48.3% were confirmed ESBLs-pE. The highest ratio of ESBLs-PE was observed in adult ward (66.7%) and laundry unit effluent (58.8%). The highest ESBL producers were E. coli (21.8%) and K. pneumoniae (4.8%). The most elevated resistance level of ESBL producer were observed to cefotaxime (95.8%) and amoxacilline/clavunalate (93%). 64% of tested Enterobacteriaceae isolates were multi drug resistant (MDR).
Conclusion

Higher magnitude of MDR and ESBLs-pE were present in the hospital wastewater. Majority of them were in adult ward and laundry unit effluents. The most frequent ESBLs-pE was among E .coli and K. pneumoniae. Hence, Consistent infection prevention and control procedures should be in practice at each ward/unit.
Soil Nitrate Mediates the Responses of Plant Community Production to the Frequency of N Addition in a Temperate Grassland: A Decadal Field Experiment
Authors: Song Changchun , Zhang Yuqiu , Ren Zhengru , Lu Haining , Chen Xu , Liu Ruoxuan , Chen Jungang , Zhang Yunhai 
Published: November 2021

Abstract
PurposeNitrogen (N) enrichment through either artificial N application or atmospheric N deposition often increases ecosystem aboveground net primary productivity (ANPP). Therefore, results from N addition experiments have been used to assess the effects of atmospheric N deposition on ecosystems. However, the frequency of atmospheric N deposition is higher than that of artificial N addition. Whether the frequency of N addition alters the long-term response of ecosystem ANPP remains unclear. MethodsWe conducted a N addition frequency experiment from 2010 in a temperate grassland, northern China. Plant community ANPP was collected in 2019 and 2020, and soil physicochemical properties were measured in 2020. ResultsPlant community ANPP was significantly enhanced by N addition, whereas these increments declined with the frequency of N addition. The responses of the grasses ANPP to the frequency of N addition were similar to those of the plant community ANPP. Forbs ANPP was not significantly altered by the frequency of N addition. Meanwhile, soil ammonium and nitrate (NO3−–N) concentrations decreased with increasing N addition frequency, while the soil water content (SWC) and pH were similar among the frequencies of N addition. Moreover, SWC and soil NO3−–N jointly promoted grasses ANPP, ultimately increasing the plant community ANPP. ConclusionOur findings extend the water and N co-limitation hypothesis by specifying the preference for NO3−–N in arid/semi-arid regions. This study also illustrates that a higher frequency of N addition is more suitable for assessing the long-term impacts of atmospheric N deposition on ecosystems.
Current Clinical Practice for Thromboprophylaxis Management in Patients With Cushing’s Syndrome Across Reference Centers of the European Reference Network on Rare Endocrine Conditions (Endo-ERN)
Authors: Haalen Femke van , Kaya M. , Pelsma I.C.M. , Dekkers O.M. , Biermasz N.R. , Cannegieter S.C. , Huisman M.V. , Vlijmen B.J.M. van , Feelders R.A. , Klok F.A. , Pereira A.M. 
Published: November 2021

Abstract
Background: Cushing’s syndrome (CS) is associated with an hypercoagulable state and an increased risk of venous thromboembolism (VTE). Evidence-based guidelines on thromboprophylaxis strategies in patients with CS are currently lacking. We aimed to map the current clinical practice for thromboprophylaxis management in patients with CS across reference centers (RCs) of the European Reference Network on Rare Endocrine Conditions (Endo-ERN), which are endorsed specifically for the diagnosis and treatment of CS. Using the EU survey tool, a primary screening survey, and subsequently a secondary, more in-depth survey were developed. Results: The majority of the RCs provided thromboprophylaxis to patients with CS (n=23/25), although only one center had a standardized thromboprophylaxis protocol (n=1/23). RCs most frequently started thromboprophylaxis from CS diagnosis onwards (n=11/23), and the majority stopped thromboprophylaxis based on individual patient characteristics, rather than standardized treatment duration (n=15/23). Factors influencing the initiation of thromboprophylaxis were ‘medical history of VTE’ (n=15/23) and ‘severity of hypercortisolism’ (n=15/23). Low-Molecular-Weight-Heparin was selected as the first-choice anticoagulant drug for thromboprophylaxis by all RCs (n=23/23). Postoperatively, the majority of RCs reported ‘severe immobilization’ as an indication to start thromboprophylaxis in patients with CS (n=15/25). Most RCs (n=19/25) did not provide standardized testing for variables of hemostasis in the postoperative care of CS. Furthermore, the majority of the RCs provided preoperative medical treatment to patients with CS (n=23/25). About half of these RCs (n=12/23) took a previous VTE into account when starting preoperative medical treatment, and about two-thirds (n=15/23) included ‘reduction of VTE risk’ as a goal of treatment. Conclusions: There is a large practice variation regarding thromboprophylaxis management and perioperative medical treatment in patients with CS, even in Endo-ERN RCs. Randomized controlled trials are needed to establish the optimal prophylactic anticoagulant regimen, carefully balancing the increased risk of (perioperative) bleeding, and the presence of additional risk factors for thrombosis.
The Role of miRNA-424 in Various Cancers: Focusing on Drug Resistance and Sensitivity
Authors: Najafi Fatemeh , Karimi Shohreh , Kazemi Bahareh , Foruzandeh Zahra , Vakili Sajjad , Seif Farhad , Derakhshani Mahdi , Solali Saeed , Alivand MohammadReza 
Published: November 2021

Abstract
However, advanced technologies have been developed in the treatment of various cancers the mortality rate of this disease is still very high. Drug resistance is a major problem for cancer patients which causes the treatment process to fail. In addition to inhibiting drug resistance, targeted therapy is also very important in its treatment. Nowadays, miRNAs have gained increasing interest in recent years which play a major role in both drug resistance and target therapy. MicroRNA (miRNA) is an important part of non-coding RNA that regulates gene expression at a posttranscriptional level. One of these microRNAs is miR-424 that by targeting genes involved in various cellular processes can participate in proliferation, differentiation, apoptosis, invasion, angiogenesis, and drug resistance and sensitivity. In this study, we collected the role of miR-424 in a variety of cancers and by identifying the role of miR-424 in drug resistance we can reduce the effect of drug resistance in many cancers.
Immunoglobulin-Induced Aseptic Meningitis in Juvenile Dermatomyositis: A Case Report
Authors: CHAN Oi Man , Kuok Chon In , Yeung Hoi Man Roanna , Chan Winnie Kwai Yu 
Published: November 2021

Abstract
Background: Aseptic meningitis is a known but unusual serious adverse effect of intravenous immunoglobulin (IVIG). It usually resembles infectious meningitis which makes diagnosis challenging. Case presentation: We report a five-and-a-half-year-old Chinese girl with juvenile dermatomyositis presented with signs of meningismus 21 hours after the initiation of IVIG infusion. Her blood work at diagnosis showed neutrophilia and lymphopenia. The cerebrospinal fluid analysis demonstrated neutrophilic pleocytosis, hyperproteinorrachia and normoglycorrhachia. All microbiological tests were negative. The child recovered fully within 72hours without neurological sequelae.Conclusion: IVIG-induced aseptic meningitis remains a diagnosis of exclusion. Although it is rare, paediatricians should be aware of this complication and avoid unnecessary investigation or treatment.
Effectiveness of the Fixtoe Device® in Plantar Pressure Reduction: A Preliminary Study.
Authors: Ruiz-Ramos Maria , Orejana-García Ángel Manuel , Vives-Merino Ignacio , Bravo-Llatas Carmen , Lázaro-Martínez José Luis , Molines-Barroso Raúl Juan 
Published: November 2021

Abstract
Background: Metatarsalgia is a common foot condition. The metatarsophalangeal stabilizing taping technique described by Yu et al. has shown good clinical results as a provisional treatment in propulsive metatarsalgia. 35 The Fixtoe Device®, a novel orthopedic device, intends to simulate stabilizing tape. However, to date, there is no evidence of its effectiveness.Methods: The aim of this study was to assess plantar pressure changes using the Fixtoe Device®, in comparison with the traditional method (stabilizing tape) in a young, healthy sample thorough a cross-sectional study. Maximal pressure (Kpa) and pressure-time integral (Kpa/s) in the second metatarsal head were measured in twenty-four healthy volunteers. Registers were taken in four different conditions: barefoot, traditional stabilizing tape, Fixtoe Device® without metatarsal pad, and Fixtoe Device® with metatarsal pad. Results: Mean second metatarsal head maximal pressure and mean pressure-time integral showed statistical difference among the four analyzed conditions (p < 0.0001 in both cases). The improvement in maximal pressure and pressure-time integral obtained in each intervention also showed significance (p < 0.0001 in both cases). Comparing the improvement of the Fixtoe Device® with and without metatarsal pad with that of tape condition showed a moderate to high and moderate effect size for both peak pressure and pressure-time integral reduction.Conclusions: The Fixtoe Device® reduces median maximal pressure and median pressure-time integral under the second metatarsal head in healthy young individuals. The Fixtoe Device® shows higher effectiveness than the traditional second metatarsophalangeal joint stabilizing taping technique. To our knowledge, this is the first investigation proving the effectiveness of the recently developed Fixtoe Device® in terms of plantar pressure modification, which leads the way to its use in clinics.
Temporary Cable Force Monitoring Techniques During Bridge Construction-phase. The Tajo River Viaduct Experience
Authors: Gaute-Alonso Álvaro , Garcia-Sanchez David , Cobo Carlos Alonso , Calderon-Uriszar-Aldaca Iñigo 
Published: November 2021

Abstract
This article addresses the comparative analysis of current techniques for monitoring cable forces. Additionally, the experience of three cable force different monitoring techniques for strain control in bridge cables during the construction phase is included: a) installing load cells on the active anchorages of the cables, b) installing unidirectional strain gauges, and c) assessing the stress on cables by applying the vibrating wire technique through the installation of accelerometers. The main advantages and disadvantages of each technique analyzed are highlighted in the construction process context of the Tajo Viaduct, one of the most singular viaducts recently built in Spain.
Morphological, physiological, and biochemical responses of onion (Allium cepa L.) breeding lines to single and combined salt and drought stresses
Authors: GÖKÇE Zahide Neslihan ÖZTÜRK , Gokce Ali Fuat , Junaid Muhammad Daniyal , Chaudhry Usman Khalid 
Published: November 2021

Abstract
Abiotic stresses deteriorate plant growth resulting in devastating yield losses. Salt stress solely cause ionic toxicity and disturbed homeostasis, whereas combined salt and drought stress has more pronounced effects on plants. Here we performed a pot experiment using 32 Turkish onion breeding lines and commercial cultivars for screening against salt, drought and combined salt and drought stress treatments initiated at the bulbification stage under greenhouse conditions. Physiological characteristics, such as gaseous exchange traits, chlorophyll index, leaf temperature, and morphological traits including number of leaves, length and diameter of leaf were measured during stress treatment and yield response of the onions was quantified by measuring bulb length, bulb diameter and bulb weight after harvest. Proline and malondialdehyde accumulation were estimated for biochemical effect of stress on onion genotypes. All genotypes responded differentially to the applied single and combined stresses. Overall results revealed that the breeding lines K25, U6, U17 and commercial cultivar K58, were grouped as sensitive across all stresses, whereas the breeding lines K41, U47, U49 and commercial cultivar K52, were found to be the most stable upon stress. These resilient genotypes can be used as breeding material for future abiotic stress studies.
LncRNA GAS5 Inhibits Temozolomide Chemoresistance to Glioma Via Inactivating Wnt/β-Catenin Pathway by Interacting with PTBP1
Authors: He Xin , Sheng Jie , Chen Yingxi , Long Xuan , Yu Wei , LIU QIAN 
Published: November 2021

Abstract
Purpose Temozolomide-based therapeutic resistance has become the crucial cause of chemotherapy failure in glioma treatment. Long non-coding RNA (lncRNA) growth arrest-specific 5 (GAS5) is reported to be downregulated in glioma and to inhibit tumor progression and metastasis. This study aimed to investigate function and potential regulatory mechanism of GAS5 in temozolomide (TMZ) chemoresistance to glioma.Methods qRT-PCR, western blotting and immunofluorescence were used to measure the levels of GAS5 and proteins. RNA-binding protein immunoprecipitation assay was used to analyze the interaction between GAS5 and PTBP1. Flow cytometry apoptosis assay, CCK-8 assay, colony formation assay and nude mice xenograft experiments were used to detect the effects of GAS5 on TMZ resistance in glioma.Results Downregulation of GAS5 might predict a poor prognosis in glioma patients. Overexpression of GAS5 improves the sensitivity to TMZ in glioma cells. Mechanistically, GAS5 could interact with polypyrimidine tract binding protein 1 (PTBP1) to downregulate its expression, thereby inactivating the Wnt/β-catenin pathway. Moreover, GAS5 could increase the anti-tumor effect of TMZ in vivo. Conclusion This study indicated that GAS5 contributed to TMZ chemoresistance of glioma through interacting with PTBP1, and then inhibiting Wnt/β-catenin pathway, which provides a novel approach to develop promising therapeutic strategy.
Subchronic Acephate Exposure Induces Immunotoxicity in White Leghorn Cockerels
Authors: Tripathi Syamantak , Govindappa Prem , Bedekar Megha , Sahni Yash , Sarkhel B. , Sharma Rajesh , Sankhala Laxmi , Shetty Raghavendra 
Published: November 2021

Abstract
We investigated the subchronic immunotoxicity of the phosphoramidothioate organophosphorous insecticide, acephate in white leghorn cockerels (WLH). The cockerels were divided into five groups; C1 (plain control), C2 (vehicle control), T1, T2, and T3 which received acephate suspended groundnut oil for 60 days at doses of 21.3, 28.4 and 42.6 mgkg−1respectively. The live body weight gain, absolute and relative weights of the spleen, thymus, and bursa of Fabricius, hemoglobin (Hb), total erythrocyte counts (TEC), packed cell volume (PCV) and lymphocytes were significantly decreased. However, monocytes, eosinophils, heterophils, and basophils were significantly increased. Total protein, albumin and albumin to globin ratio, the antibody response to RD-F and delayed-type hypersensitivity response to DNCB dye or PHA-P, erythrocyte and brain Acetylcholinesterase activity was also significantly reduced in T2 and T3. At 40 and 60 days of acephate exposure, nitrate and nitric oxide production by RD-F and mitogen Con A stimulated peripheral blood and splenic lymphocytes, as well as lymphocyte proliferation in response to antigen RD-F and mitogen Con A stimulation, were significantly decreased in groups T2 and T3. Furthermore, dose-dependent increases in the frequency of micronuclei formation, varying intensity serum protein bands with different protein fractions (14.85KDa), and splenic DNA laddering (180 bp) were observed in groups T2 and T3. Histopathologically, the spleen and bursa showed morphological changes and mild lymphocyte depletion. In conclusion, low-level acephate exposure may affect acetylcholinesterase, lymphocytes, and immune responses in cockerels. As a result, it should be considered when assessing immunotoxicity and the risk to human and animal health.
The continuity of prime numbers can lead to even continuity(Goldbach conjecture)
Authors: Ling Xie 
Published: November 2021

Abstract
Two prime numbers{3,5}.{3,5}→{3+3= 6，3+5=8，5+5=10} →{6,8,10}.{{10}→(5+5=10 = 3 + 7) → 7}Increased by 7 →{3, 5, 7}.Note changes:{3,5}→{ 6,8,10}→ {3, 5, 7}.{3,5,7}→{ 3+3= 6, 3+5=8, 5+5=10, 5+7=12, 7+7=14}→{ 6,8,10,12,14}.{{14}→(7+7=14 = 3 + 11) → 11} Increased by 11 →{3, 5, 7, 11}.Note changes: {3,5,7}→{ 6,8,10,12,14}→ {3, 5, 7,11}. The same logic would be:{3,5,7,11}→{ 6,8,10,12,14,16}→ {3, 5, 7,11,13}.The same logic would be:{3,5,7,11,13}→{ 6,8,10,12,14,16,18,20}→ {3, 5, 7,11,13,17}.【Note: if a prime number is added, n consecutive even numbers (n≥1) can be obtained.】Sequential continuation, ......If you expand infinitely in the above specified mode: {3, 5, 7, 11, 13, 17, 19, 23,...Get: { 6,8,10,12,14,16,18,20,22,...The above is: the continuity of prime numbers can lead to even continuity.Get: Goldbach conjecture holds. If it is mandatory: Authenticity stops at an even number 2n.{{3, 5, 7, 11, 13, 17, 19, 23,...,p1}→{ 6,8,10,12,14,16,18,20,22,...,2n}.{3, 5, 7, 11, 13, 17, 19, 23,...,p1} ↛(2n+2).∀p+∀p≠2n+2 } It can be proved that: It violates the "Bertrand Chebyshev" theorem.∴ {3, 5, 7, 11, 13, 17, 19, 23,...→{ 6,8,10,12,14,16,18,20,22,...Get: Goldbach conjecture holds.
Comprehensive Analysis of Immune-Related Metabolic Genes in Lung Adenocarcinoma
Authors: Li Fangfang , Huang Chun , Qiu Llingxiao , Li Ping , Zhang Guojun 
Published: November 2021

Abstract
Purpose

The immunotherapy of lung adenocarcinoma has received more and more attention. Different immune cells can affect other metabolic genes and lifespan, and cell metabolism directly regulates immune cell functions. Therefore, it is crucial to explore the role of immune-related metabolic genes in lung adenocarcinoma.
Methods

In this study, we divided immune-related metabolic genes into three categories based on different immune characteristics and researched immune and clinical pathology. LASSO regression analysis was used to screen immune-related metabolic genes, and a clinical prediction model of the screened genes was constructed. Finally, we selected the intersection of immune metabolism genes that are highly expressed in the tumor site and immune metabolism genes that are negatively related to survival, and used qRT-PCR for experimental verification.
Results

We first screened out immune-related metabolic genes that may affect lung cancer tumor progression, and screened out 9 pivot genes (TK1, TCN1, CAV1, ACMSD, HS3ST2, HS3ST5, AMN, ADRA2C, ACOXL) through LASSO regression analysis and constructed Prognosis model. Finally, through the screening of tumor-related immune metabolism genes, we obtained five pivot genes (HMMR, PFKP, RRM2, TCN1 and TK1). Our qRT-PCR results also show that RRM2 is positively correlated with CDK2, CDK4, CDK6, and CDK8, revealing the close relationship between RRM2 and immune cell tumor infiltration.
Conclusion

We conducted a comprehensive analysis of the immune infiltration of the tumor microenvironment of lung cancer, and finally determined RRM2 as a promising immune metabolism checkpoint for lung adenocarcinoma based on the high correlation of RRM2 with immune cells and CDK family.