bluuebunny/crossref_metadata_2025_split huggingface dataset:
Nasal Septal Abscesses: A Systematic Review
Authors: Shaari Ariana L. , Patil Disha , Youssef Victoria , Bebawy George , Shaari Diana S. , Schwartz Cynthia , Eloy Jean Anderson 
Published: January 2025

Background:
Nasal septal abscesses (NSA) necessitate prompt recognition and management to prevent morbidity and long-term sequelae. To date, no comprehensive review of NSA alone has been conducted.

Objective:
To conduct a systematic review of the presentation and management of NSA and determine patients at risk of sequelae.

Methods:
Preferred Reporting Items for Systematic Review and Meta-Analysis (PRISMA) guidelines were followed. PubMed, Web of Science, Cochrane, and reference lists were searched. Patient demographic information, etiology, presentation, management, and outcomes were recorded and analyzed.

Results:
A total of 79 articles (90 cases) were included. The mean age was 32.86 years old with a slight male predominance. The most common signs were nasal pain (44.86%, N=48), trouble breathing (23.36%, N=25), and headache (15.89%, N=17). All cases were treated with intranasal drainage. Most patients (66.67%, N=60) were asymptomatic at last follow-up. Most abscesses (73.07%) were located in the anterior septum. Of those patients who experienced complications, common complications were saddle nose deformity (70.59%, N=24), nasal obstruction (5.88%, N=2), and septal perforation (5.88%, N=2). No significant association between age (P = 0.23), sex (P = 0.99), history of diabetes (P = 0.11), history of nasal trauma (P = 0.91), history of nasal/dental surgery (P = 0.14), location (P = 0.18), or postoperative nasal packing (P = 0.65) and NSA outcomes was found.

Conclusion:
Patients with or without a history of immunodeficiency, trauma, or nasal surgery are at risk of developing NSA. Without adequate treatment, NSA can be associated with functional and aesthetic sequelae.
Tattoo ink induces inflammation in the draining lymph node and impairs the immune response against a COVID-19 vaccine
Authors: Capucetti Arianna , Falivene Juliana , Pizzichetti Chiara , Latino Irene , Mazzucchelli Luca , Schacht Vivien , Hauri Urs , Raimondi Andrea , Virgilio Tommaso , Pulfer Alain , Mosole Simone , Grau-Roma Llorenec , Bäumler Wolfgang , Palus Martin , Renner Louis , Ruzek Daniel , Levy Gabrielle Goldman , Foerster Milena , Chahine Kamil , Gonzalez Santiago F. 
Published: January 2025

AbstractDespite safety concerns regarding the toxicity of tattoo ink, no studies have reported the consequences of tattooing on the immune response. In this work, we have characterized the transport and accumulation of different tattoo inks in the lymphatic system using a murine model. Upon quick lymphatic drainage, we observed that macrophages mainly capture the ink in the lymph node (LN). An initial inflammatory reaction at local and systemic levels follows ink capture. Notably, the inflammatory process is maintained over time as we observed clear signs of inflammation in the draining LN two months following tattooing. In addition, the capture of ink by macrophages was associated with the induction of apoptosis in both human and murine models. Furthermore, the ink accumulated in the LN altered the immune response against a COVID-19 vaccine. We observed a reduced antibody response following vaccination with a mRNA-based SARS-CoV-2 vaccine, which was associated with a decreased expression of the Spike protein in macrophages in the draining LN. Considering the unstoppable trend of tattooing in the population, our results are crucial in informing the toxicology programs, policymakers, and the general public regarding the potential risk of the tattooing practice associated with an altered immune response.
Language comprehension developmental milestones in typically developing children assessed by the new Language Phenotype Assessment (LPA)
Authors: Vyshedskiy Andrey , Pevzner Ariella , Mack Brigid , Shrayer Eva , Zea Miranda , Bunner Sasha , Wong Nicole , Baskina Elena , Sheikh Amira , Tagliavia Alessandro , Fucks Andriane Schmiedel , Santos Andressa Schmiedel Sanches , Poloni Lucas Ernesto Pavoski , Fucks Elielton , Bolotovsky Yudit , Kang Sung Jin (Sam) 
Published: January 2025

AbstractWe recently identified three distinct phenotypes of language comprehension in 31,000 autistic individuals1: 1) individuals with the Command Phenotype were limited to comprehension of simple commands; 2) individuals with the Modifier Phenotype demonstrated additional comprehension of color, size, and number modifiers; and 3) individuals with the Syntactic Phenotype added comprehension of spatial prepositions, verb tenses, flexible syntax, possessive pronouns, complex explanations, and fairytales. We hypothesized that typically developing children progress through the same three language comprehension phenotypes and aimed to investigate the typical age at which each phenotype emerged. To evaluate comprehension in young children, we developed a new assessment, the 15-item Language Phenotype Assessment (LPA), which utilizes toy-animal manipulatives to avoid reliance on picture interpretation and includes short instructions to reduce auditory memory load. The LPA was administered to 116 typically-developing children aged 1.5 to 7 years. Results revealed a developmental pattern in line with the three previously described phenotypes: 50% of typically developing children attained the Command Phenotype by 1.5-years, the Modifier Phenotype by 3.0-years, and the Syntactic Phenotype by 3.7-years-of-age. Future research should focus on establishing normative data for the LPA to enable earlier identification of language comprehension challenges, facilitating timely initiation of language interventions.
Development and quality assessment of low-cost benchtop malting protocol for laboratory-scale malt quality evaluation
Authors: Rani Heena , Standish Andrew , Walling Jason G , Whitcomb Sarah J 
Published: January 2025

ABSTRACTHigh-quality malt is influenced by three primary factors: barley genotype, environmental conditions, and malting process. To effectively evaluate malting barley breeding material and assess how environmental changes influence malt quality, it is essential to have laboratory- scale malting methods that can produce malt approximating that produced by commercial malting operations. However, existing laboratory-scale malting procedures often demand large quantities of grain, rely on specialized equipment, and are costly. To overcome these challenges, we developed a small sample-scale benchtop malting method utilizing standard laboratory equipment and components available at hardware stores. We validated the method by conducting standard malt quality tests including diastatic power, α-amylase activity, total malt protein, and wort composition (soluble protein, wort soluble/total malt protein, β-glucan, free amino nitrogen, and malt extract). Our findings indicate that the benchtop malting method yields quality metrics comparable to those obtained from established small-scale and full-scale malting protocols. Furthermore, a key innovation of this system is the use of separate Erlenmeyer flasks for malting each sample. Unlike conventional shared malting systems, this design enables precise measurement and comparison of treatment effects across samples malted simultaneously. This reliable, low-cost, and efficient method provides a valuable tool for screening malt quality traits in breeding lines with limited sample sizes and for testing malting regimes aimed at improving malt quality and efficiency. Additionally, it offers an accessible solution for producing high-quality, research-scale malt in laboratories without dedicated quality assurance facilities.
Burnout Syndrome and coping mechanisms among Prison Officers at Nsawam Medium Security Prison, Eastern Region, Ghana
Authors: Djoletoe Florence , Tabong Philip Teg-Nefaah 
Published: January 2025

AbstractWorkplace burnout negatively affects employees’ productivity, organizational effectiveness, social stability, and personal health and performance. Burnout is a syndrome comprising emotional exhaustion, depersonalization, and loss of personal accomplishment. In Ghana, there are several studies on job-related burnout among health workers but very little on this syndrome in different professional groups like prison officers. Thus, this study assessed burnout syndrome, associated factors, and coping strategies among Prison Officers at Nsawam Medium Security Prison. This was a cross-sectional study involving 266 prison officers from the Nsawam Medium Security Prison. A self-administered questionnaire was used to collect data from participants. Data was collected on sociodemographic characteristics, workplace environment, coping strategies, and burnout. The Maslach Burnout Inventory-Human Services scale was adopted for this study. The data was analysed using STATA 16. Descriptive Statistical methods were used to summarize socio-demographic characteristics of participants. Bivariable analysis using Pearson’s Chi Square was used to determine the association between demographic and work-related factors and burnout. Multivariate logistic regression was used to determine factors associated with burnout at 95% significance level. The prevalence of burnout was 9%. The main causes of burnout perceived by POs were irregular shifts (71.6%), heavy workload (49.0%) and lack of training and opportunities for development (47.7%). Male prison officers had 69% reduction in the odds of burnout compared to their female counterparts (AOR: 0.31; 95% CI= 0.13-0.75, p=0.009). Also, prison officers with diploma/certificate had 89% reduction in odds to burnout compared to those with postgraduate degrees (AOR: 0.11; 95% CI= 0.02-0.69, p=0.019). Talking to colleagues (62.5%), looking forward to off duty (60.1%), talking to family (54.3%), and keeping thoughts and feelings to oneself (42.1%) were the dominant coping strategies. Overall, the prevalence of burnout among prison officers in this study was low. Heavy workloads, erratic shift schedules, and a lack of training and career development chances contribute to prison officers’ burnout. Additionally, shift allocations need to be changed to lessen officer burnout.
Genetic Modifiers of Prenatal Brain Injury After Zika Virus Infection: A Scoping Review
Authors: Marques Fernanda J P , Ruan Janet , Razal Rozel B. , Leyser Marcio , Kousa Youssef A. 
Published: January 2025

AbstractIntroductionThe severity of virally induced prenatal brain injury, even among dizygotic twins, varies according to individual and maternal risk and protective factors, including genomics.ObjectiveThis scoping review aims to analyze data on genetic susceptibility to neurological outcomes in children exposed in utero to Zika virus.MethodsWe followed JBI methodology for this scoping review. A search in PubMed, Scopus, CINAHL, Web of Science, Academic Search Ultimate, Agricola, Health Source: Nursing/Academic Edition, and Psychology and Behavioral Sciences Collection was conducted. Three reviewers independently screened studies using the Rayyan platform. Studies on gene mutations impacting brain injury after Zika virus infection were included.ResultsThirteen articles identifying candidate genes related to brain injury were reviewed. Twenty-three genes were implicated in modulating susceptibility to prenatal brain injury, including six maternal and 17 infant genes.ConclusionMaternal and fetal genetic factors likely contribute susceptibility to virally induced prenatal brain injury. Analyzing polygenic risk could aid in future screening programs to identify individuals at risk. This information may eventually be integrated into clinical data, helping healthcare providers, families, and patients understand how to personalize care for better outcomes.ImpactThis paper evaluates available evidence about the relationship between genetic susceptibility and neurological consequences of Zika virus exposure during pregnancy.After performing a scoping review, we identified 13 articles describing candidate genes that potentially contribute to the development of virally induced brain injury after prenatal Zika infection. Of the genes identified, six were associated with maternal risks, while 17 were linked to the fetus.Maternal and prenatal genetic factors could increase the risk of virally induced prenatal brain injury.Future research should investigate factors that can modify disease pathogenesis toward the goal of reducing the global impact of brain injury.
The Role of GLP-1 Receptor Agonists in Managing Cancer Therapy-Related Cardiac Dysfunction
Authors: Vignarajah Aravinthan , Kim San , Albliwi Moath , Ahn Hyunjun Max , Izda Aleksandar , Naffa Faris , Vigneswaramoorthy Nishanthi , Barot Shimoli , Shah Gautam 
Published: January 2025

ABSTRACTBackgroundAnti-cancer therapies currently available can lead to cancer therapy-related cardiac dysfunction (CTRCD). Glucagon-like peptide-1 receptor agonists (GLP-1 RAs) have been shown to exhibit cardioprotective effects but its impact on CTRCD has not been evaluated.ObjectivesThis study evaluates the impact of GLP-1 RAs on all-cause mortality, hospitalization, and heart failure exacerbation in patients with CTRCD.MethodsThis is a retrospective cohort analysis using the TriNetX research network. Patients aged ≥18 years with a history of cancer who received antineoplastic therapies and subsequently developed cancer therapy-related cardiac dysfunction (CTRCD) between January 1, 2012, and January 1, 2023, were included. Patients receiving guideline-directed medical therapy (GDMT) for heart failure were divided into two groups: those using GLP-1 RAs and those not using them. Propensity score matching (1:1) was applied based on demographics, comorbidities, and medications, resulting in a matched cohort of 1,223 patients. Outcomes over a 1-year follow-up were measured.ResultsThe study cohort found 12,410 patients with CTRCD of which 1,223 had received GLP-1 RA treatment (mean age: 66.4 years; 47.3% female; 65.9% White). Patients receiving GLP-1 RAs in addition to GDMT had a significantly lower risk of acute heart failure exacerbations, all-cause mortality, and all-cause hospitalization (OR: 0.566 [95% CI: 0.48-0.668]; P < 0.001). A clinically significant reduction in atrial fibrillation/flutter and ventricular tachycardia was observed in the GLP-1 RA groups, although did not reach statistical significance.ConclusionIn patients with CTRCD, adding GLP-1 RA therapy to GDMT significantly improves mortality and heart failure outcomes over 1 year.
Time from onset to aneurysm securing is associated with rebleeding in patients with aneurysmal subarachnoid hemorrhage
Authors: Daurel Emile , Le Gall Arthur , Charamel-Lenain Claire , Ledos Alexandre , Eugène François , Launey Yoann 
Published: January 2025

AbstractRebleeding is a major complication in patients admitted for aneurysmal subarachnoid hemorrhage. The optimal timing for aneurysm securing after the initial headache remains debated. The objective of this study was to evaluate the previously established predictive factors for rebleeding in a contemporary retrospective cohort (2020-2022, Rennes University Hospital). Among the 226 patients included in the analysis, 33 (15%) experienced rebleeding. The mean time-to-event (rebleeding or securing) was significantly shorter in patients without rebleeding compared with patients with rebleeding (36 ± 3vs.63 ± 9 hours, respectively, p<0.001). For each additional hour of delay since the first symptoms, the odds of rebleeding increased by 0.7% (OR = 1.007 [1.001-1.013], p=0.026). This association remains significant in multivariate analysis. Despite its limitations, this study highlights the importance of early management, taking into account the time elapsed since the initial headache.
Enhanced CB1 receptor function in GABAergic neurons mediates hyperexcitability and impaired sensory-driven synchrony of cortical circuits in Fragile X Syndrome model mice
Authors: Gonzalez D , Jonak CR , Bernabucci M , Molinaro G , Collins K , Assad SA , Gibson JR , Binder DK , Huber KM 
Published: January 2025

AbstractElectroencephalographic (EEG) recordings in individuals with Fragile X Syndrome (FXS) and the mouse model of FXS (Fmr1KO) display cortical hyperexcitability at rest, as well as deficits in sensory-driven cortical network synchrony. A form of circuit hyperexcitability is observed inex vivocortical slices ofFmr1KO mice as prolonged persistent activity, or Up, states. It is unknown if the circuit mechanisms that cause prolonged Up states contribute to FXS-relevant EEG phenotypes. Here we examined the role of endocannabinoids (eCB) in prolonged Up states in slices and resting and sensory-driven EEG phenotypes in awakeFmr1KO mice. Bidirectional changes in eCB function are reported in theFmr1KO that depend on synapse type (excitatory or inhibitory). We demonstrate that pharmacological or genetic reduction of Cannabinoid Receptor 1 (CB1R) in GABAergic neurons rescues prolonged cortical Up states and deficits in sensory-driven cortical synchrony inFmr1KO mice. In support of these findings, recordings fromFmr1KO cortical Layer (L) 2/3 pyramidal neurons revealed enhanced CB1R-mediated suppression of inhibitory synaptic currents. In contrast, genetic reduction ofCnr1in glutamatergic neurons did not affect Up state duration, but deletion ofFmr1in the same neurons was sufficient to cause long Up states. These findings support a model where loss ofFmr1in glutamatergic neurons leads to enhanced CB1R-mediated suppression of GABAergic synaptic transmission, prolonged cortical circuit activation and reduced sensory-driven circuit synchronization. Results suggest that antagonism of CB1Rs as a therapeutic strategy to correct sensory processing deficits in FXS.
Targeting Osteosarcoma heterogeneity to improve therapeutic response
Authors: Melano Brenda , Dinh Truc , Zirak Bahar , Karimzadeh Mehran , Woo Brian J , Choi Benedict , Goodarzi Hani , Sweet-Cordero E. Alejandro 
Published: January 2025

SummaryIntra tumor heterogeneity complicates cancer therapy by providing tumors with the ability to alter their phenotypes and become more therapy resistant. Here, we tested the hypothesis that identifying and modulating expression of key state-specific transcription factors could be used as a strategy for driving cells to a more therapy-sensitive state. Recent single-cell studies have explored the inter and intra tumoral heterogeneity of osteosarcoma and identified gene pathways enriched in specific cell states. For example, metastatic tumors are characterized by an expression of genes in the TNF-α, PI3K, TGFß and mTOR pathways. We identified similar profiles in osteosarcoma patient-derived xenograft-derived cell lines and potential transcription factor drivers of these states. We then used perturb-seq to downregulate expression of key transcription factors and evaluated the effect of these modulations on single cell RNA profiles and drug responses. Knockdown ofNFE2L3orNR0B1increased the proportion of cells sensitive to targeted therapy. This approach, which could potentially be applied to other cancers, could be used as a strategy to increase the response to targeted therapies by increasing the proportion of cells in a drug-sensitive state.HighlightsDistinct transcriptomic states were identified in osteosarcoma cell lines using single-cell RNA sequencing.Lineage tracing identified states with differential sensitivity to therapy.Using perturb-seq, we identified transcription factors that drive cells towards a more sensitive state.The transcription factors NR0B1 and NFE2L3 were identified as targets capable of reprogramming cells to a sensitive state.
Theift140-Deficient Zebrafish: A Model for Renal Cystogenesis and an F0-Based Screen to Identify Genetic Modifiers of Kidney Cysts
Authors: Zhu Ping , Lavin Andrew , Ding Yonghe , Xu Xiaolei , Lin Xueying 
Published: January 2025

AbstractGenetic modifiers are believed to play an important role in the onset and severity of polycystic kidney disease (PKD), but identifying these modifiers has been challenging due to the lack of effective methodologies. In this study, we investigated zebrafish mutants ofIFT140, a newly identified ADPKD gene, and observed phenotypes similar to those seen in mammalian models, including kidney cysts and bone defects. Using efficient microhomology-mediated end joining (MMEJ)-based genome editing technology, we demonstrated thatift140CRISPRants recapitulate mutant phenotypes while bypassing the early lethality of the mutants to allow for renal cyst analysis in adult fish. In addition to cilia defects, non-cilia phenotypes, such as disrupted cell polarity and aberrant microtubule stabilization in kidney epithelial cells, may also contribute toift140-associated cystogenesis. Importantly, the ability to detectift140-associated renal cysts with ease allowed us to develop an F0-based genetic screen to identify potential protective modifiers. A pilot screen of sixteen genes previously linked to dysregulated signaling pathways in ADPKD revealed both known and novel modifiers, includingmtorandulk1a. Inhibition ofmtorandulk1areversed both cilia-related and non-cilia-related abnormalities. In summary, our study underscores the potential of using zebrafish as a model for the efficient discovery of genetic modifiers of kidney cysts.
Revealing the diversity of light-harvesting antenna of in vivo photosystem I
Authors: Zhang Xianjun , Taniguchi Rin , Ye Shen , Shibata Yutaka 
Published: January 2025

AbstractThe photosynthetic reaction is driven by the cooperation of two light-excited pigment-protein supercomplexes: photosystem II (PSII) and photosystem I (PSI). The efficiency of the excitation of the two PSs relies on the exquisite organization of their light-harvesting antenna under environmental fluctuations. However, since the antenna-protein composition within cells remains elusive, the in vivo events arising from antenna variations cannot be accurately explored. Here, we implemented the single-pixel excitation-emission spectroscopy ofChlamydomonascells under 80 K using a cryogenic optical microscope. The antenna variations of in vivo PSI can be exclusively evaluated via this low-temperature spectro-imaging method. The simultaneous acquisition of two types of fluorescence spectra enables the analysis of the intracellular correlation between the PSII/PSI intensity ratio and the chlorophyll-b/a(Chl-b/a) concentration ratio. We found that the Chl-b/aratio hardly correlated with the PSII/PSI intensity ratio in most cases, suggesting that the in vivo PSI intensity ratio reflects the relative PSI stoichiometry rather than their antenna sizes. More importantly, the analysis of the PSI antenna-related Chl-bconcentration within cells reveals a mega-antenna system that is much larger than the antenna sizes of the PSI supercomplexes whose structures have been resolved so far. Such PSI megacomplexes tended to be enriched in the region surrounding the pyrenoids ofChlamydomonascells. We anticipate the present investigation to be a starting point for directly estimating the arrangements of antenna systems of photosystems at the single-cell scale, which is necessary for a deeper understanding of dynamic in vivo events related to the photosynthetic light-harvesting process.