bluuebunny/crossref_metadata_2025_split huggingface dataset:
Activation of GPR39 With TC-G 1008 Attenuates Neuroinflammation Via SIRT1/PGC-1α/Nrf2 Pathway Post Neonatal Hypoxic-ischemic Injury in Rats
Authors: Xie Shucai , Jiang Xili , Doycheva Desislava Met , Shi Hui , Jin Peng , Gao Ling , Liu Rui , Xiao Jie , Hu Xiao , Tang Jiping , Zhang Lina , Zhang John H 
Published: July 2021

Abstract
Background: Hypoxic-ischemic encephalopathy (HIE) is a severe anoxic brain injury that leads to premature mortality or long-term disabilities in infants. Neuroinflammation is a vital contributor to the pathogenic cascade post HIE and a mediator to secondary neuronal death. As a plasma membrane G-protein coupled receptor, GPR39, exhibits anti-inflammatory activity in several diseases. This study aimed to explore the neuroprotective function of GPR39 through inhibition of inflammation post hypoxic-ischemic (HI) injury and to elaborate the contribution of sirtuin 1(SIRT1)/ peroxisome proliferator-activated receptor-γ coactivator 1α (PGC-1α)/ nuclear factor, erythroid 2 like 2(Nrf2) in G protein-coupled receptor 39 (GPR39)-mediated protection.Methods: A total of 206 10-day old Sprague Dawley rat pups were subjected to HIE or sham surgery. TC-G 1008 was administered intranasally at 1h, 25h, 49h, and 73h post HIE induction. SIRT1 inhibitor EX527, GPR39 CRISPR, and PGC-1α CRISPR were administered to elucidate the underlying mechanisms. Brain infarct area, short-term and long-term neurobehavioral tests, Nissl staining, western blot, and immunofluorescence staining were performed post HIE.Results: The expression of GPR39 and pathway-related proteins, SIRT1、PGC-1α and Nrf2 were increased in a time-dependent manner, peaking at 24 h or 48h post HIE. Intranasal administration of TC-G 1008 reduced the percent infarcted area and improved short-term and long-term neurological deficits. Moreover, TC-G 1008 treatment significantly increased the expression of SIRT1, PGC-1α, Nrf2, IL-6, IL-1β, and TNF-α. GPR39 CRISPR EX527 and PGC-1α CRISPR abolished GPR39’s neuroprotective effects post HIE.Conclusions:TC-G 1008 attenuated neuroinflammation in part via the SIRT1/PGC-1α/Nrf2 pathway in a neonatal rat model of HIE. TC-G 1008 may be a novel therapeutic target for treatment post neonatal HIE injury.
Knockdown of LSD1 alleviates the IL-1β-induced chondrocyte apoptosis, inflammation and ECM degradation via TRIM32-mediated autophagy
Authors: Xu Wenqiang , Liu Xiaofeng , Qu Wenqing , Wang Xin , Su Hao , Li Wenliang , Chen Yiheng 
Published: July 2021

Abstract
Osteoarthritis (OA) is a common joint disease with characteristics of chronic inflammation and articular cartilage degeneration. It has been proved that LSD1 was up-regulated in OA cartilage tissues, but its role and regulatory mechanism in OA are unclear. Herein, interleukin 1 beta (IL-1β)-treated human chondrocytes was performed as a cell model of OA. Then, LSD1 expression was found that up-regulated in OA cartilage tissues and IL-1β-induced chondrocytes. Knockdown of LSD1 increased cell viability, while decreased apoptosis rate and inflammatory cytokines secretion levels in IL-1β-induced chondrocytes. In addition, knockdown of LSD1 reduced the expression of catabolic proteins (MMP-13 and ADAMTS-5) and enhanced the expression of anabolic proteins (Collagen II and Aggrecan) in chondrocytes after IL-1β stimulation. Moreover, overexpression of TRIM32 repressed chondrocyte viability, while promoted IL-1β-induced chondrocyte apoptosis, inflammation and ECM degradation. The expression of LSD1 and TRIM32 in OA cartilage was positively correlated, and knockdown of LSD1 down-regulated TRIM32 expression of chondrocytes. Our data further indicated that LSD1 regulated autophagy of chondrocytes through modulating TRIM32. Overexpression of TRIM32 reduced the effect of LSD1 knockdown on IL-1β-induced chondrocytes, while activating autophagy by Rapamycin further reversed this reduction. Therefore, our study shows that knockdown of LSD1 inhibited IL-1β-induced chondrocyte apoptosis, inflammation and ECM degradation via TRIM32-mediated autophagy.
Fabrication and Detailed Characterization of Perlite Mineral Supported a New Magnetic Nanomaterial.
Authors: KUTLUAY Sinan , ŞAHİN Ömer , ECE MEHMET ŞAKİR 
Published: July 2021

Abstract
Today, the possibilities offered by nanotechnology are undeniable. Nanotechnology has permeated all aspects of our daily lives. Nanotechnology, as an interdisciplinary field, also provides unique products in a variety of engineering fields. These products are more appealing than macro products due to their unique properties such as surface area, volume, conductivity, optical, and magnetic. Magnetite (Fe3O4), the most powerful magnetic mineral on the planet, has many properties such as nano size, surface area, morphology, and ferromagnetism at room temperature, and thus Fe3O4-based products are being developed for appropriate studies. There are very few materials created by modifying perlite Fe3O4, a mineral abundant in nature. In this study, Fe3O4 was modified with the mineral perlite to create magnetic nanomaterials. To that end, Fe3O4/Perlite magnetic nanomaterials were created using a co-precipitation method, followed by Fe3O4/Perlite@SiO2 magnetic nanomaterials created using a sol-gel method, and Fe3O4/Perlite@SiO2@Sulfanilamide magnetic nanomaterials created using a sol-gel method. The structures of the synthesized magnetic nano materials were clarified by various characterization devices such as BET, FTIR, SEM, SEM-EDX, TGA-DTA, and XRD. The characterization findings revealed that the syntheses and surface coatings of Fe3O4/Perlite, Fe3O4/Perlite@SiO2 and Fe3O4/Perlite@SiO2@Sulfanilamide magnetic nanomaterials were successfully performed. Furthermore, in light of these findings, Fe3O4/Perlite, Fe3O4/Perlite@SiO2 and Fe3O4/Perlite@SiO2@Sulfanilamide magnetic nano materials were discovered to be in crystalline cubic spinel form, mechanical and thermal stability, and mesoporic size. Since synthesized perlite-supported magnetic nano materials have potential in many applications, such as sustainable technologies, environmental safety technologies such as water reclamation and pollution removal, adsorption technologies, etc., they are brought to the attention of related fields.
Sex Differences in Blood Pressure and the Kidney Cortex Transcriptome in Nonhuman Primates
Authors: Riojas Angelica M , Spradling-Reeves Kimberly D. , Shade Robert E. , Puppala Sobha R. , Christensen Clinton L. , Birnbaum Shifra , Glenn Jeremy P. , Hall-Ursone Shannan , Cox Laura A. 
Published: July 2021

Abstract
Hypertension is a complex disease influenced by sex, and genetic and environmental factors. Blood pressure (BP) is a continuous trait that is heritable in primates, including humans and baboons. The kidneys play a role in systemically regulating BP. Sex differences in BP onset and control with antihypertensive drug therapies have been observed in humans and rodents. Hypertension studies in nonhuman primates (NHP) to date have focused on males. We hypothesized that there are differences in renal molecular networks associated with BP in female and male primates. Sodium-naïve female (n=8) and male (n=9) baboons were fed a low-sodium chow diet prior to and during the study. Implantable telemetry devices continuously monitored heart rate and blood pressure over 24-hours, and ultrasound-guided kidney biopsies were collected for RNA-Seq. Serum 17 beta-estradiol concentration correlated BP in females. BP in males correlated with Na+ intake, blood urea nitrogen, and glucose. Cell type composition of renal biopsies was consistent between females and males. Sex differences were observed in the kidney transcriptomes by principal components analysis and weighted gene co-expression network analysis. Network analysis revealed HNF4A, ESR1, ESR2, SMARCA4, TP53, and NR3C1 as BP regulators in males. Our results demonstrate sex differences in primate kidney molecular networks and provide evidence of a novel link between renal transcription factors and BP regulation in males. Understanding sex differences and transcriptome variation in primate kidneys correlated with BP and clinical measures associated with BP will inform better therapies towards the goal of precision medicine for women and men.
Nrf2 Activation Attenuates Chronic Constriction Injury-induced Neuropathic Pain via Induction of PGC-1α-mediated Mitochondrial Biogenesis in the Spinal Cord
Authors: Sun Jia , Li Jia-Yan , Zhang Long-Qing , Li Dan-Yang , Wu Jia-Yi , Gao Shao-Jie , Zhou Ya-Qun , Mei Wei 
Published: July 2021

Abstract
BackgroundNeuropathic pain is a debilitating disease with few effective treatments. Emerging evidence indicates the involvement of mitochondrial dysfunction and oxidative stress in neuropathic pain. Nuclear factor erythroid 2-related factor 2 (Nrf2) is a potent regulator of antioxidant response system. In this study, we investigated whether RTA-408 (a novel synthetic triterpenoid under clinical investigation) could activate Nrf2 and promote mitochondrial biogenesis (MB) to reverse neuropathic pain and the underlying mechanisms.MethodsNeuropathic pain was induced by chronic constriction injury (CCI) of the sciatic nerve. Pain behaviors were measured via the von-Frey test and Hargreaves plantar test. The L4-6 spinal cord was collected to examine the activation of Nrf2 and MB.ResultsRTA-408 treatment significantly reversed mechanical allodynia and thermal hyperalgesia in CCI mice in a dose-dependent manner. Furthermore, RTA-408 increased the activity of Nrf2 and significantly restored MB that was impaired in CCI mice in an Nrf2 dependent manner. Peroxisome proliferator-activated receptor-gamma coactivator-1alpha (PGC-1α) is the key regulator of MB. We found that PGC-1α activator also exhibited a potent analgesic effect in CCI mice. Moreover, the antinociceptive effect of RTA-408 was reversed by the pre-injection of PGC-1α inhibitor.ConclusionsNrf2 activation attenuates chronic constriction injury-induced neuropathic pain via induction of PGC-1α-mediated mitochondrial biogenesis in the spinal cord. Our results indicate that Nrf2 may be a potential therapeutic strategy to ameliorate neuropathic pain and many other disorders with oxidative stress and mitochondrial dysfunction.
Effect of Co-solvent on the Performance of LDHs Photocatalytic Degradation of Methyl Orange Solution
Authors: Huang Liang , Jin Xianghui , Tao xumei , Yang Chen , Han Yuanyuan 
Published: July 2021

Abstract
In this paper, NiAlCe hydrotalcite was prepared by co-precipitation method and hydrothermal method in different volume fractions of ethylene glycol-water mixed solvent and polyethylene glycol-water mixed solvent, and the effect of photocatalytic degradation of methyl orange under ultraviolet light was studied. Samples were characterized by XRD, XPS, UV-Vis and FT-IR in order to find the reasons for the improvement of photocatalytic performance under co-solvent system. NiAlCe-10%EG(h) and NiAlCe-15%PEG(h) had smaller particle size, which was beneficial to photocatalytic performance. The degradation rate of methyl orange could reach up to 100% in 42 min using NiAlCe hydrotalcite with 10% volume fraction of EG and in 48 min using NiAlCe hydrotalcite with 15% volume fraction of PEG. The photocatalytic effect of NiAlCe hydrotalcite samples prepared by hydrothermal method were better than that prepared by co-precipitation method. This provided a new type of catalyst for the degradation of dyes: preparing with ethylene glycol-water mixed solvent.
TRIB3 Regulates FSHR Expression in Human Granulosa Cells Under High Levels of Free Fatty Acids
Authors: Wang Nan , Si Chenchen , Xia Lan , Wu Xian , Zhao Sheng , Xu Huihui , Ding Zhide , Niu Zhihong 
Published: July 2021

Abstract
Background: Granulosa cells (GCs) in cumulus oophorus highly express follicle stimulating hormone receptor (FSHR), which is the most important mediator of both estradiol synthesis and oocyte maturation. Obese women have elevated free fatty acids (FFAs) levels in their follicular fluids and decreased FSHR expression in GCs, which is related to an altered protein kinase B/glycogen synthase kinase 3β (Akt/GSK3β) signaling pathway. Such FFA increases accompany 3-fold rises in pseudokinase 3 (TRIB3) expression and reduce the Akt phosphorylation status in both the human liver and in insulinoma cell lines. Therefore, in a high FFA environment, we determined if TRIB3 mediates regulation of FSHR via the Akt/GSK3β signaling pathway in human GCs. Methods: GCs from women undergoing in vitro fertilization were collected and designated as high and low FFAs cohorts based on their follicular fluid FFA content. GCs with low FFA levels and a human granulosa-like tumor (KGN) cell line were exposed to palmitic acid (PA), which is a dominate FFA follicular fluid constituent. The effects were assessed of this substitution on the Akt/GSK3β signaling pathway activity as well as the expressions of TRIB3 and FSHR at both the gene and protein levels by qPCR, Western blot and immunofluorescence staining analyses. Meanwhile, the individual effects of TRIB3 knockdown in KGN cells and p-AKT inhibitors were compared to determine the mechanisms of FFA-induced FSHR downregulation.Results: The average FSH dose consuming per oocyte (FSH dose/oocyte) was elevated and Top embryo quality ratio was decreased in women with high levels of FFAs in their follicular fluid. In these women, the GC TRIB3 and ATF4 protein expression levels were upregulated which was accompanied by FSHR downregulation. Such upregulation was confirmed based on corresponding increases in their gene expression levels. On the other hand, the levels of p-Akt decreased while p-GSK3β increased in the GCs. Moreover, TRIB3 knockdown reversed declines in FSHR expression and estradiol (E2) production in KGN cells treated with PA, which also resulted in increased p-Akt levels and declines in the p-GSK3β level. In contrast, treatment of TRIB3-knockdown cells with an inhibitor of p-Akt (Ser473) resulted in rises in the levels of both p-GSK3β as well as FSHR expression whereas E2 synthesis fell. Conclusions: During exposure to a high FFA content, TRIB3 can reduce FSHR expression through stimulation of the Akt/GSK3β pathway in human GCs. This response may contribute to inducing oocyte maturation.
Finite Element Modelling and Simulating Effects of Hairiness Performance on Hairiness Entanglement During Fabric Pilling
Authors: Xiao Qi , Wang Rui , Sun Hongyu , Wang Jingru 
Published: July 2021

Abstract
For analyzing behaviors of hairiness entanglement during fabric pilling, nonlinear dynamic motion equations are deduced based on the elastic thin rod element, combined with the moving characteristics of hairiness, which follow the principles of mechanical equilibrium and energy conservation. The finite element simulation model of the effects of hairiness performance on behaviors of hairiness entanglement was established by ABAQUS. The analysis solution values of nonlinear dynamics were compared with the finite element simulation results. The results showed that hairiness elastic modulus, hairiness friction coefficient and hairiness diameter have significant effects on frictional dissipation energy, strain energy and kinetic energy produced by hairiness entanglement during pilling. Compared the finite element simulation results with analysis solution values, they are in good agreement. The fitness is greater than 0.96, which verifies the validity of finite element method.
Title Anterior Prominence of the Femoral Condyle Varies among Prosthesis Designs and Surgical Techniques in Total Knee Arthroplasty
Authors: Itou Junya , Kuwashima Umito , Itoh Masafumi , Okazaki Ken 
Published: July 2021

Abstract
BackgroundPatellofemoral overstuffing after total knee arthroplasty can cause limited range of motion and anterior knee pain. This study compared anterior prominence of femoral components among different prothesis designs in surgical simulation models utilizing the anterior reference (AR) and posterior reference (PR) techniques.MethodsSurgical simulations were performed using preoperative computed tomography data of 30 patients on a three-dimensional planning system. Four implant models were used: Attune, Persona, Journey II, and Legion. Rotational alignment was set parallel to the transepicondylar axis and size selection was based on absence of notch formation in the femoral anterior cortex and best fit to the shape of the medial posterior femoral condyle. For each combination of surgical technique (AR or PR method) and implant model, measurements were taken of the maximum medial, central, and lateral prominence of the implant from the anterior femoral cortex (mm).ResultsUsing either the AR or PR method, the medial and central prominences were significantly lower with Journey II than with other models. The lateral prominence was the lowest with Attune in the AR method. The AR method was associated with significantly less prominence than the PR method, regardless of implant model.ConclusionsDegree of anterior prominence of the femoral implant is affected by implant design when the AR method is used. The PR method is associated with greater anterior prominence compared with the AR method, with size pitch an additional influencing factor. Journey II is associated with the least anterior prominence when using either method.
Operational Definition of Complementary, Alternative, and Integrative Medicine Derived from a Systematic Search
Authors: Ng Jeremy Y. , Dhawan Tushar , Dogadova Ekaterina , Taghi-Zada Zhala , Vacca Alexandra , Wieland L. Susan , Moher David 
Published: July 2021

Abstract
Background
Identifying what therapies constitute complementary, alternative, and/or integrative medicine (CAIM) is complex for a multitude of reasons. An operational definition is dynamic, and changes based on both historical time period and geographical location whereby many jurisdictions may integrate or consider their traditional system(s) of medicine as conventional care. To date, only one operational definition of “complementary and alternative medicine” has been proposed, by Cochrane researchers in 2011. This definition is not only several years old but also did not use systematic methods to compile the therapies. Furthermore, it did not capture the concept “integrative medicine”, which is an increasingly popular aspect of the use of complementary therapies in practice. An updated operational definition reflective of CAIM is warranted given the rapidly increasing body of CAIM research literature published each year.
Methods
Four peer-reviewed or otherwise quality-assessed information resource types were used to inform the development of the operational definition: peer-reviewed articles resulting from searches across seven academic databases; the “aims and scope” webpages of peer-reviewed CAIM journals; CAIM entries found in online encyclopedias, and highly-ranked websites identified through searches of CAIM-related terms on HONcode. Screening of eligible resources, and data extraction of CAIM therapies across them, were each conducted independently and in duplicate. CAIM therapies across eligible sources were deduplicated.
Results
A total of 101 eligible resources were identified: peer-reviewed articles (n = 19), journal “aims and scope” webpages (n = 22), encyclopedia entries (n = 11), HONcode-searched websites (n = 49). Six hundred four unique CAIM terms were included in this operational definition of CAIM.
Conclusions
This updated operational definition is the first to be informed by systematic methods, and could support the harmonization of CAIM-related research through the provision of a standard of classification, as well as support improved collaboration between different research groups.
Sistem Informasi Manajemen Kinerja Guru
Authors: Oktiagraha Windy Puji , Proboyekti Umi , Oslan Yetli 
Published: July 2021

Penjaminan mutu pendidikan saat ini dianggap penting untuk dilakukan oleh setiap sekolah. Proses yang terjadi dalam penjaminan mutu pendidikan ini diantaranya adalah penilaian kinerja guru. Kinerja guru di sekolah dapat dinilai kehadiran harian, total jam mengajar, dan penilaian siswa. Dalam pelaksanaannya, sekolah mendapati permasalahan efisiensi dalam penggunaan sumber daya biaya dan waktu. Data-data yang digunakan sebagai dasar penilaian sebagaianterekam dalam media tercetak. Sebagian lagi dalam format elektronik dan tidak menjadi satu kesatuan. Akses dan pengolahan data-data tersebut menjadi tidak efisien dan membutuhkan waktu banyak. Media terintegrasi yang mampu menampung seluruh penilaian tersebut ke dalam satu tempat belum tersedia. Dari permasalahan tersebut penulis membuat aplikasi berbasis web untuk sekolah menengah atas dengan tujuan untuk membantu proses penilaian kinerja guru. Aplikasi ini dirancang agar mampu menjadi media pencatatan, pengolahan dan pengelolaan data yang terintegrasi untuk tiap aspek penilaian kinerja guru. Sistem yang dibangun akan memfasilitasi guru dalam melakukan presensi secara digital.Selain itu, sistem dapat memfasilitasi murid dalam mengisi form survei untuk penilaian guru. Dari data-data ini sistem juga akan menampilkan nilai untuk tiap-tiap aspek yang berguna bagi kepala sekolah untuk pengambilan keputusan. Proses pengujian sistem ini dilakukan berdasarkan beberapa skenario yang ditujukan untuk responden penelitian. Responden pengujian dalam sistem ini berjumlah 10 orang. Adapun responden terdiri dari 1 orang sebagai waka kurikulum, 1 orang sebagai kepala sekolah, 7 orang sebagai guru dan 1 orang sebagai siswa. Hasil akhir dari penelitian ini menunjukkan bahwa seluruh responden dalam penelitian berhasil melakukan task-task dalam skenario yang dibuat.
The distribution pattern and ecological restoration technology of aquatic plants in a eutrophic water landscape belt
Authors: Wang Sirong 
Published: July 2021

Abstract
Taking the eutrophic Taige canal and Caoqiao River in the upper reaches of North Taihu Lake as the experimental objects, 12 monitoring points were set up and data were recorded. The distribution pattern of aquatic plants in the water landscape belt was studied by variance/mean ratio, aggregation intensity index, negative binomial parameter, Cassie index and α–diversity index. Based on the principle of aquatic plants repairing eutrophic water, a suitable water environment was selected. The effect of ecological restoration was studied by photometry. The results showed that: the main aquatic plant communities in this area evolved from submerged to floating leaf and emergent plants, which shows that the vertical distribution pattern of the aquatic plant communities was greatly affected by hydrology; the distribution pattern of aquatic plant diversity showed a good single curve in the vertical direction and a single curve in the horizontal direction; the results of ecological restoration showed that the average removal rates of total nitrogen (TN) and total phosphorus (TP) by mixing the three plants were 86.76% and 93.89%, respectively. Among them, the best combination for TN removal was water hyacinth (Eichhornia crassipes) + lytchus (Lythrum salicaria) + calamus (Acorus calamus), and the best combination for TP removal was water hyacinth + lytchus + hibiscus (Nelumbonucifera).