bluuebunny/crossref_metadata_2025_split huggingface dataset:
Bioresources and diversity of snakehead, Channa striata (Bloch 1793): a proposed model for optimal and sustainable utilization of freshwater fish
Authors: Gustiano R , Kurniawan K , Kusmini I I 
Published: May 2021

AbstractAmong freshwater fishes in Indonesia, snakehead is an essential and valuable fish bioresources for a long time. Although breeding snakehead just started in the last decade, direct consumption, raw material for food industry and pharmacy/albumin source have already been developed earlier. This study outlines snakehead biological resources and their diversity, production trends and challenges, and understanding for strategic planning for its optimal and sustainable use. Of the 10 snakehead species in Indonesia,Channa striata is the most popular species. Although Indonesia’s snakehead production contributes significantly to global production, the production of this species in the last three decades still depends on inland fisheries around 73-97%, and the rest comes from aquaculture. Therefore, the decline in snakehead production occurs because of over-exploitation, seasonal influence and high vulnerability of the species to climate change. Bioresource flow model (BRFM) is proposed to optimize the use of snakehead to provide strategic planning for further development. This model includes a domestication program for aquaculture and conservation, hatchery production, an alternative understanding of snakehead aquaculture production systems, biotechnological improvement processing for albumin production, and wastewater treatment management.
Botanical inventory and rarity of the fern Genus Pteris in the karst forests of Bantimurung - Bulusaraung National Park, Sulawesi – Indonesia
Authors: Praptosuwiryo Titien Ngatinem 
Published: May 2021

Abstract
The ferns flora of Sulawesi is poorly documented. Pteris L. is a large fern genus inhabiting various habitats, mostly forests, which are distributed in tropical and subtropical countries. Floristic study on fern genus Pteris in the karst forests of Bantimurung Bulusaraung National Park (BabulNP) was carried out to add our knowledge of the ferns flora of Sulawesi. This study aimed to inventory Pteris growing in the karst forest of BabelNP and determine the rarity of each species. Nine species were recorded, namely, Pteris biaurita L., P. ensiformis Burm.f., P. longipes D. Don, P. pellucida C. Presl, P. radicans Christ., P. quadriaurita Retz., P. spinescens C. Presl., P. tripartita Swartz, and P. vittata L.. P. pellucida and P. spinescens may be new records for Sulawesi. Summary of the most important characters for distinguishing species of Pteris in the park is provided. Five species, namely Pteris longipes, P. pellucida, P. quadriaurita, P. radicans, and P. spinescens are considered as rare species. This study contributes to the knowledge of Malesian biodiversity, especially for fern flora of Sulawesi growing on karst forest. This survey provides baseline data for one of the most dominant elements of the understory cover in the limestone forests of BabulNP.
The impact of pine forest management on the surrounding community in Malang Forest Management Unit
Authors: Adalina Y , Sawitri R 
Published: May 2021

Abstract
Pine forest used by the community surrounding forest to improve the community’s economy through Community Forest Management Program established by Perum Perhutani with division of arable land and plantation of species vegetation. This study aims to: (1) determine the diversity of plants in pine forest; (2) to analyze socioeconomic of the land user farmers, and (3) to recognize the optimization of land use and the diversity of cultivated vegetables. The research was conducted at the Junggo Forest Resort Production, Forest Management Unit Malang, East Java, Indonesia. The survey method was carried out through a plant inventory with total of 23 plots. Farmers were interviewed from 30 respondents through a structured questionnaire and the data were analyzed descriptively qualitatively. There were five species of stands found at tree canopy level namely Eucalyptus alba, Albizia sp, Toona sureni, Persea americana, and Pinus merkussi species with the highest. Various vegetables that combined in 2-3 species, implementing an agroforestry system. The average of land user’s income is IDR 4,500,000 - IDR 6,000,000 per 4 months of the harvest season. The implementation of Community Forest Management Program under pine stands would provide benefits for improving welfare of communities around the forest.
Genetic purity and population structure of potato variety Granola L
Authors: Kirana R , Kusmana  , Karjadi A K , Gunadi N , Reflinur  , Hermanto C 
Published: May 2021

Abstract
The phenotypic performances have formed the basis for genetic purity evaluation. However, the phenotypic based the genetic purity assessment are time-consuming, expensive, and not stable due to a great environmental effect on traits expression. While, the genotypic based genetic purity evaluation of a variety offers an efficient, more stable, and precise result than those phenotypic method due to no environmental factors involved. The objectives of this study were to determine genetic purity and population structure of Granola L. potato variety derived from twelve collection sources using microsatellite markers. This study consisted of following steps, such as cultivation of Granola L. potato varieties in the field, DNA isolation, PCR analysis, amplified DNA fragment analysis. The results showed that Granola L. variety derived from twelve sources had high level of genetic purity which indicated by genetic similarity value of 0.96 observed in present study. Based on population structure analysis, Granola L. variety might have consisted of two sub-populations of which one sub-population was more dominant than other sub-population. Therefore, genetic purification activities for Granola L. variety are still needed to increase genetic purity and to reduce phenotypic differences in future.
Naringin may alleviate doxorubicin cytotoxic effects in C2C12 myoblast cells
Authors: Septisetyani E P , Prasetyaningrum P W , Santoso A 
Published: May 2021

Abstract
Doxorubicin is one of the first line chemotherapeutic agents used to treat different types of cancer. However, despite its efficacy, doxorubicin can be toxic to muscle tissue and worsen the prognosis of cancer patients. Previously, we investigated the effect of chemopreventive agents curcumin, naringin and epigallocathecin-3-gallate (EGCG), which show anticancer properties in cancer cells, in C2C12 myoblast cells. We found that naringin, a citrus flavonoid, showed no significant cytotoxic effect on C2C12 cells. In this study, we investigated doxorubicin cytotoxicity alone or in combination with naringin against C2C12 myoblast cells, which can be differentiated into multinucleated myotubes. Cell viability assays had been carried out by WST assay, whereas C2C12 differentiation was observed after incubation of C2C12 cells with 2% horse serum and stained with crystal violet. As a result, C2C12 cell viability decreased to 43.89% after treatment with 2 µM doxorubicin for 24 hours. Moreover, C2C12 differentiation is also inhibited by doxorubicin. In contrast, C2C12 cell viability was maintained at 90.45% after treatment with 500 µM naringin. Interestingly, naringin may alleviate doxorubicin cytotoxic effects on C2C12 cell myogenesis.
Antimicrobial activity assay of Plantaricin F produced by L. lactis pNZ8148-plnAF against Candida albicans
Authors: Mustopa A Z , Rasmentari N , Kusdianawati  , Triratna L , Nurfatwa M 
Published: May 2021

Abstract
Plantaricin is a bacteriocin produced by Lactobacillus plantarum. It has potential as probiotic, antimicrobial, easily degraded by proteolytic enzyme and Generally Recognized as Safe (GRAS). The objective of this study was to produce plantaricin F expressed from Lactococcus lactis pNZ8148-plnAF, purified using gel-filtration chromatography and investigated its antimicrobial activity using disc diffusion method against Candida albicans using nystatin as a positive control. Crude plantaricin F yield was 1.23 grams. Gel-filtration chromatography using Sephadex G-50 of plantaricin F yielding in 25 fractions. 5 fractions (fraction no 7, 9, 17, 18, 19) from 25 fractions have antimicrobial activities based on the clear zone on disc diffusion method.
The commodity of Curcuma spp. sold in the traditional markets of Yogyakarta
Authors: Jalil M , Purwantoro A , Daryono B S , Kurniawan F Y , Purnomo  
Published: May 2021

Abstract

Curcuma is a member of the Zingiberaceae family. In the traditional market, Curcuma soldby empon-empon trader. Previous research reported that some Curcuma species are rare to be found and were sold by traders. Therefore, there is a concern regarding the possibility of Curcuma spp. extinction. Research objectives are: (1) to find the number of empon-empon traders in Yogyakarta; (2) to know the percentage of the number of rhizomes found in traditional markets in Yogyakarta; and (3) to find whether the Curcuma species are found completely in the Yogyakarta traditional market. The research was conducted by exploration of traditional market in Yogyakarta. The data were analyzed by the Cochran test. Research results showed that the percentages of rhizomes are as follow: temu ireng (C. aeruginosa) (27.59%), temu giring (C. heyneana)(31.03%), turmeric (C. domestica) (93.10%), temu mangga (C. mangga) (27.59%), temu glenyeh (C. soloensis) (0%), temu lawak (C. xanthorrhiza) (64.29%), and temu putih (C. zedoaria) (17.86%), sold by empon-empon traders. Some Curcuma were rare in nature such as C. mangga, C. heyneana, C. purpuracens, C. soloensis, C. euchroma, C. colorata, and C. borg. The rhizome of Curcuma spp. is found to be incomplete in the traditional markets of Yogyakarta.
Micropropagation of diploid and tetraploid Plectranthus amboinicus (Lour.) Spreng
Authors: Sari L , Rudiyanto  , Hafiizh E A , Ermayanti T M 
Published: May 2021

Abstract

Plectranthus amboinicus (Lour.) Spreng is commonly consumed by people in North Sumatra, Indonesia, as a vegetable, which is commonly called Torbangun. Plant tissue culture is an alternative method for the mass-production of transplants. Genetic improvement by somatic cell manipulation such as induction of polyploid plants is needed to improve biomass production as well as their secondary metabolite products. This research aimed to conduct in vitro micropropagation of diploid and tetraploid P. amboinicus on Murashige and Skoog (MS) medium containing BAP or Kinetin. Shoot tips were used as explants and cultured on MS medium containing BAP or Kinetin at 0, 0.5, 1 and 2 mg/l given as single concentration. The experiment used a completely randomized design with four replications. Each replication consisted of three explants. The results showed that height of shoots was affected by type of cytokinins. Kinetin at 0.5 mg/l gave the highest shoots of tetraploid plants significantly different with addition of BAP both to diploid and tetraploid plants. The number of multiple shoots and leaves varied in both diploid and tetraploid plants. Root formation was best on the medium without addition of cytokinins. Both diploid and tetraploid plantlets had a high survival rate after acclimatization in the greenhouse.
The potency of coconut shell wood vinegar and essential oils as botanical insecticides to control Hypothenemus hampei (Coleoptera: Curculionidae)
Authors: Indriati G , Samsudin  , Susilawati  , Puspitasari M 
Published: May 2021

Abstract

Hypothenemus hampei is the major pest on a coffee plantation invested coffee berry in the field then unwittingly carried to storage. The aim of this research was to evaluate the potential of coconut shell wood vinegar and essential oils to control H. hampei. The research conducted in the Pest and Disease Laboratory of IIBCRI and Pakuwon Experimental Field started from January until December 2018. The test of clove oil and citronella oil residue conducted at 0, 1, 2, 3, 4, and 5 days after treatment (DAT) at a concentration of 2%; 3%; 4%; 5%; control (aquadest) and α-cyhalothrin insecticides (2 ml/l), respectively. Each treatment used 15 adults of H. hampei replicated 3 times. Coconut shell wood vinegar formulated into 2 formulas. Each formula was applied with different essential oil concentrations as 6 treatments and replicated 4 times. Result showed that the botanical insecticide formula of coconut shell wood vinegar + 5% clove oil caused mortality of H. hampei until 80 - 95%, and coconut shell wood vinegar + 5% citronella oil caused mortality of H. hampei reached 73.34 - 88.33%. Overall, the best botanical insecticide formula is a combination of coconut shell wood vinegar + clove oil.
Efficiency of Agrobacterium tumefaciens-mediated transformation of tobacco (Nicotiana tabacum L.) with rice OsNAC6 gene
Authors: Rachmat A , Chairunisa  , Maulana B S 
Published: May 2021

Abstract
Crop improvement by genetic engineering requires efficient plant regeneration derived from cultured cells or tissues. The success of cell or tissue culture regeneration depends on reliable explant source. In this study, three different types of explant (callus, stem, and leaf) of tobacco (Nicotiana tabacum L.) were evaluated to determine the most effective explant source for Osnac6 gene transformation. OsNAC6 encodes a transcription factor belonged to NAC transcription factor family in rice (Oryza sativa L.). OsNAC6 transcription factor has been reported in some studies to confer tolerance to drought stress. Agrobacterium tumefaciens strain EHA 105 harboring pC1305 plasmid containing the OsNAC6 gene driven by CaMV 35S promoter (pARNAC6) was used for transformation. The T-DNA contains hygromycin phosphotransferase (hpt) gene as a selectable marker. PCR confirmed the presence of the hpt gene in the T0 generation of the transformed tobacco plants. The PCR result showed that a total of 50 tobacco plants were positive for hpt gene which 18, 20, and 12 of them derived from callus, stem, and leaf explants respectively. The highest transformation efficiency was obtained by using the stem as explant material, i.e. 17%. Meanwhile, the obtained transformation efficiency by using callus and leaf was 15% and 10% respectively.
In silico screening of oil palm early and continuously flowering gene candidates for faster breeding program
Authors: Polosoro A , Enggarini W , Hadiarto T , Supena E D J , Suharsono  
Published: May 2021

Abstract
Oil palm (Elaeis guineensis Jacq.) is a vegetable oil-producing crop used for consumption and biodiesel production. Fastrack breeding is one of breeding techniques to reduce plant vegetative phase significantly by a combination of conventional and transgene technologies. However, the lack information of flowering genes in oil palm become a gap for developing this technology. This paper aimed to discover the candidate genes from oil palm for developing early flowering oil palm lines. Screening potential florigen protein by comparing rice OsHd3a protein sequence in the NCBI database successfully discovered 13 florigen protein candidates with more than 53% similarity and up to 90% query cover. All protein candidates were analyzed by protein structure homology modeling (SWISS-MODEL) based on several parameters, including GMQE score and QMEAN Z-score. The modeling discovered that at least eight proteins predicted a similar structure to AtFT1 and four proteins are related to AtTFL1, which repress flowering process. Based on GMQE, QMEAN Z-score, and phylogenetic tree, we suggested three proteins (XP_010911427, XP_010925712, and XP_019705101) as the strong candidates for florigen protein. However, to verify their functions, all selected proteins should be inserted to the model plant and evaluate them as morphologically and physiologically.
Microbial lipid production by the yeast Lipomyces starkeyi InaCC Y604 grown on various carbon sources
Authors: Juanssilfero A B , Salsabila P , Agustriana E , Andriani A , Fahrurrozi  , Perwitasari U , Sutrisno A 
Published: May 2021

Abstract
The use of non-renewable fuels in the transportation sector boosts the development of renewable biofuels, such as biodiesel. However, crops-based biodiesel production can cause the increment of deforestation and greenhouse gas emissions. This circumstance motivates the development of oleaginous microorganisms as alternative raw materials to overcome these problems. Lipomyces starkeyi is one of the potential oleaginous microorganisms due to its ability to produce higher lipid content. In addition, L. starkeyi is capable to grow in affordable carbon sources, such as lignocellulose and molases, which can reduce the production costs. The aim of this study is to determine the ability of L. starkeyi InaCC Y604 in producing lipids from various carbon sources and its effects on the composition of fatty acid methyl esters (FAME) and lipid contents. L. starkeyi InaCC Y604 were cultivated in nitrogen-limited mineral medium (-NMM) with various carbon sources, namely glucose, xylose, fructose, galactose, mannose, cellobiose and a mixture of glucose and xylose. The results showed that mixed glucose and xylose gave the best result with the lipid content achieved at 64.19% (w/w). The results obtained indicated that L. starkeyi InaCC Y604 could be a potential candidate for further optimizing biodiesel production.
Application of Projection Pursuit Analysis Method Based on Kernel Function in Fault Diagnosis for Rolling Bearing
Authors: Jing Cheng , Le Su , Weiqing Wang , Shan He 
Published: May 2021

Abstract
In view of nonlinear and non-Gaussian characteristics of fault feature for rolling bearing of wind turbine, the projection pursuit analysis method based on kernel function is put forward to make pattern recognition for all bearing running states. It elaborates the principle of projection pursuit method, and plan and steps for pattern recognition, then has a simulation and test by MATLAB software. The test results show that the projection pursuit analysis method based on kernel function is an effective method for fault diagnosis, and it can quickly and efficiently identify rolling bearing running states.
Design of sound monitoring and fault diagnosis system for dry-type transformers
Authors: Wu Weidong , Wu Zhengyang , Guo Shouchun , Lu Yulin , Ma Li , Wang Qinghua 
Published: May 2021

Abstract
This paper focuses on several common fault phenomena and possible causes of dry-type transformer. MATLAB programming realizes the processing of acoustic signal. Through the encapsulation of GUI interface program and the processing and analysis of a large number of fault signals, fault sets are formed. Through the actual transformer fault acoustic signal processing, the main fault frequency of the acoustic signal is extracted and used as an important criterion for transformer fault detection.
Optimization experiments of the SNCR denitrification system in circulating fluidized bed boiler after ultra-low emission transformation
Authors: Han Yuan , Zhu Yujie 
Published: May 2021

Abstract
The current work firstly aims to improve denitrification efficiency of the selective non-catalytic reduction (SNCR) denitrification system in a circulating fluidized bed (CFB) boiler after ultra-low emission transformation, to reduce the operating pressure of the subsequent selective catalytic reduction (SCR) denitrification system, and to extend the service life of the boiler. Secondly, it attempts to ensure that the concentration of nitrogen oxides (NOx) does not exceed the emission standard, to drop the consumption of the reducing agent, and to save operating costs. Therefore, some key factors, such as the number of spray guns affect denitrification efficiency and the amount of ammonia escape, were chosen as the operating parameters to be optimized. Meanwhile, a 150 MW CFB boiler in the Inner Mongolia Autonomous Region of China was employed for the experimentation. We then designed and implemented the optimization experiments of the SNCR denitrification system, including optimizing the operating parameters and deeply optimizing the SNCR denitrification process. The denitrification efficiency can reach 76.6% when there are four spray guns with valve opening of 50, 100, 100, and 50% respectively from top to bottom. The current work can be used as a frame of reference for carrying out relevant experiments on similar units.
Research and Development on the Separators of Li-ion Batteries
Authors: Wang Lanhua , Liu Qi , Xu Shufei , Du Yuju , Ren Xiaolin 
Published: May 2021

Abstract
With the rapid development of the lithium-ion batteries, the lithium-ion battery separator, as a critical component of lithium-ion batteries, had been extensively studied. In this paper, the main function and performance indicators about the separator materials, recent research and development status at home and abroad of lithium ion battery separators were reviewed. The types and existing problems of mainstream diaphragms in the existing market were described. The production methods of the separators were discussed mainly. It simply introduces the status of the separator’s modification and development of new type of battery separator. Finally, the future development trend of battery separators was forecasted.
The cooling techniques on the continuous casting process
Authors: Changyong Liu , Yajie Dong , Hua Wang , Jinping Xie , Pang Shusheng , Xu Chen 
Published: May 2021

Abstract
In the process of blast furnace ironmaking, in order to facilitate the transportation and storage of the molten iron, the liquid molten iron is continuously cast into iron block by using cast iron machine. In the process of continuous casting, adopting safe and efficient cooling method can shorten the cooling time of cast iron block, increase the cooling rate of molten iron in cast iron mold, and improve the productivity of cast iron machine. In this paper, the development of cooling technology in continuous cast iron process is reviewed, and the technical characteristics and application evolution of spray water cooling and dry cooling are introduced.
Structural evolution of rubber-wood char under different pyrolysis conditions
Authors: Liu Liang , Cao Ya , Qing Mengxia , Long Yutian 
Published: May 2021

Abstract
In order to explore the effect of different pyrolysis conditio ns on the structural characteristics of rubber wood biomass pyrolysis, rubber wood char was prepared under fast pyrolysis and slow pyrolysis conditions. The char was analyzed by BET, SEM, FT-IR and Raman spectroscopy to explore the effects of different pyrolysis conditions on pore characteristics, surface structure and chemical structure. The results showed that the nitrogen isothermal adsorption/desorption curves of slow pyrolysis at 900°C are obviously different, and the char structure has a certain amount of mesopores and macropores. With the pyrolysis temperature increased, the alkane side chain of rubber wood char broke, and formeda large number of active sites, which promoted the condensation of small aromatic rings into large aromatic ring structures, and deepened the aromatization of char structures. The rapid pyrolysis method causes the content of the small aromatic ring structure to be greater than that of the large aromatic ring structure at low temperature. The slow pyrolysis method causes the increase of the large aromatic ring structure to be greater than that of the small aromatic ring at a lower temperature contrarily.