bluuebunny/crossref_metadata_2025_split huggingface dataset:
Pembelajaran Kooperatif dengan Metode Personal Selling Presentation dan Café World: Model Pembelajaran untuk Meningkatkan Kemampuan Kognitif Memahami
Authors: Pujiastuti Indah 
Published: June 2021

Ranah kognitif “memahami” adalah tingkat kemampuan kognitif yang penting sebelum berlanjut ke jenjang koginitif yang lebih tinggi. Berdasarkan hal tersebut, penelitian ini merupakan penelitian untuk melihat proses kognitif “memahami” mahasiswa menggunakan dua model pembelajaran. Dua model pembelajaran yang dimaksud adalah model Pembelajaran Kooperatif dengan metode Personal Selling Presentation dan model pembelajaran World Cafe. Penelitian ini adalah penelitian quasi eksperimen dengan rancangan post test only control group design. Populasi penelitian ini adalah seluruh mahasiswa Prodi Pendidikan Bahasa dan Sastra Indonesia yang mengikuti mata kuliah Telaah Kurikulum dan Buku Teks, sampel yang diambil adalah keseluruhan populasi/sampel jenuh. Sampel dibagi 3 kelompok yaitu kelas I-01 sebagai kelompok eksperimen 1, kelas I-02 sebagai kelompok eksperimen 2, dan kelas I-03 sebagai kelompok kontrol. Data didapatkan melalui tes bebentuk uraian. Hasil penelitian didapatkan melalui uji hipotesis menggunakan uji anava satu jalur. Hasil menunjukkan bahwa terdapat perbedaan hasil proses kognitif “memahami” antara ketiga kelompok. Untuk melihat perbedaan lebih jauh, hasil post hoc menunjukkan bahwa kelompok eksperimen 1 dan 2 tidak memiliki perbedaan kemampuan “memahami” secara signifikan. Namun, kelompok eksperimen 1 dan 2 memiliki perbedaan yang siginifikan dengan kelompok control. Simpulannya model Pembelajaran Kooperatif dengan metode Personal Selling Presentation dan model Pembelajaran World Cafe dapat meningkatkan hasil belajar pada ranah kognitif “memahami”. Jadi, pendidik dapat memanfatkan dua model pembelajaran ini untuk kegiatan belajar yang bersifat teori atau topik pembelajaran.yang memiliki karakteristik serupa.
THE EFFECT OF THERAPY GUIDED IMAGERY ON THE LEVEL OF ANXIETY IN PATIENTS OF TYPE 2 DIABETES MELLITUS
Authors: Susanti Susanti , Ristyawati Rizka Nur Indah 
Published: June 2021

Background: Type 2 diabetes mellitus (DM) is a chronic disease that has a negative physical and psychological impact. The psychological impact such as feeling useless and depressed. It causes a high level of anxiety, which makes a person vulnerable. Thus, it will complicate the management of type 2 diabetes mellitus.
Objective: This study aims to analyze the effect of guided imagery therapy on the level of anxiety in patients with type 2 diabetes mellitus.
Methods: This study used pre-experimental designs with one group pretest-posttest design with a sample of 26 respondents taken using simple random sampling technique. Measuring the level of anxiety using the Hamilton Anxiety Rating Scale (HARS) questionnaire. Data were analyzed using the Wilcoxon statistical test.
Results: Data of respondents with DM who had anxiety before the guided imagery therapy in the mild anxiety level category were 21 people (81%), after the therapy decreased with the anticipation category as many as 21 people (81%). Obtained p-value 0.000 with α = 0.05 (p < α), indicating that there is an effect of guided imagery therapy on anxiety levels in type 2 diabetes mellitus patients in Bulak Banteng Health Center Area.
Conclusions: This study shows that applying guided imagery therapy can reduce anxiety levels in patient with type 2 diabetes mellitus. Researchers recommend that future researchers use more samples.
Penerapan Model Delikan dan CTL untuk Meningkatkan Prestasi Belajar Pancasila dan Pengembangan Karakter Mahasiswa PGSD FKIP Unib
Authors: Juarsa Osa Juarsa , Djuwita Puspa Djuwita 
Published: June 2021

Penelitian ini bertujuan menganalisis peningkatan prestasi belajar Pancasila serta ketertanaman karakter pada diri mahasiswa dan mendekripsikan strategi yang paling tepat dilakukan melalui pembelajaran model delikan, dan CTL. Metode pendekatan menggunakan kaji tindak latar kelas atau clasroon action reserch . Penelitian ini dilaksanakan di kelas 1A PGSD FKIP UNIB TA 2019/2020 dengan menggunakan sistem siklus. Setiap siklusnya terdiri atas beberapa tahapan yaitu: (1) Perencanaan (planning); (2) Pelaksanaan tindakan (action); (3) Pengamatan (observation); dan (4) Refleksi (reflection), Metode pengumpulan data yang digunakan adalah Observasi ,Tes Hasil Belajar, Wawancara. Metode Analisis Data dengan penilaian acuan patokan (PAP), analisa data hasil sebelum dan sesudah perlakuan/treatment digunakan statistik mean serta data yang tidak bisa dianalisa dengan rumus statistik akan dinarasikan kemudian diberi argumen. Hasil penelitian menunjukkan bahwa prestasi belajar mahasiswa dapat ditingkatkan dari rata-rata hasil belajar kelas 66,60 menjadi 83 serta terjadi ketertanaman karakter kedalam diri mahasiswa. Rata-rata skor hasil ceklis pengamatan perubahan karakter mahasiswa atas nilai-nilai yang terkandung dalam materi pembelajaran pada siklus 2 jauh lebik daripada pada siklus 1, dimana rata-rata skor pada siklus 1 sebesar 66,25 dan rata-rata skor pada siklus 2 sebesar 80,06. Peningkatan ini terjadi setelah mahasiswa diajak menggali nilai yang terkandung dalam materi yang dipelajari juga diajak untuk mamantau keterbiasaan melakukan, dan mengkaji dengan cara mengemukakan pertanyaan apa, mengapa, bagaimana, siapa, dimana dan kapan dilakukan serta kemana harus dipertanggungjawabkan serta dilakukan secara terus menerus.
Combining Metagenomic Sequencing With Whole Exome Sequencing to Optimize Clinical Strategies in Neonates With a Suspected Central Nervous System Infection
Authors: Ge Mengmeng , Gan Mingyu , Yan Kai , Xiao Feifan , Yang Lin , Wu Bingbing , Xiao Mili , Ba Yin , Zhang Rong , Wang Jin , Cheng Guoqiang , Wang Laishuan , Cao Yun , Zhou Wenhao , Hu Liyuan 
Published: June 2021

ObjectivesCentral nervous system (CNS) infection has a high incidence and mortality in neonates, but conventional tests are time-consuming and have a low sensitivity. Some rare genetic diseases may have some similar clinical manifestations as CNS infection. Therefore, we aimed to evaluate the performance of metagenomic next-generation sequencing (mNGS) in diagnosing neonatal CNS infection and to explore the etiology of neonatal suspected CNS infection by combining mNGS with whole exome sequencing (WES).MethodsWe prospectively enrolled neonates with a suspected CNS infection who were admitted to the neonatal intensive care unit(NICU) from September 1, 2019, to May 31, 2020. Cerebrospinal fluid (CSF) samples collected from all patients were tested by using conventional methods and mNGS. For patients with a confirmed CNS infection and patients with an unclear clinical diagnosis, WES was performed on blood samples.ResultsEighty-eight neonatal patients were enrolled, and 101 CSF samples were collected. Fourty-three blood samples were collected for WES. mNGS showed a sample diagnostic yield of 19.8% (20/101) compared to 4.95% (5/101) for the conventional methods. In the empirical treatment group, the detection rate of mNGS was significantly higher than that of conventional methods [27% vs. 6.3%, p=0.002]. Among the 88 patients, 15 patients were etiologically diagnosed by mNGS alone, five patients were etiologically identified by WES alone, and one patient was diagnosed by both mNGS and WES. Twelve of 13 diagnoses based solely on mNGS had a likely clinical effect. Six patients diagnosed by WES also experienced clinical effect.ConclusionsFor patients with a suspected CNS infections, mNGS combined with WES might significantly improve the diagnostic rate of the etiology and effectively guide clinical strategies.
Direct Flow Medical vs. Edwards Sapien 3 Prosthesis: A Propensity Matched Comparison on Intermediate Safety and Mortality
Authors: Edlinger Christoph , Bannehr Marwin , Wernly Bernhard , Kücken Tanja , Okamoto Maki , Lichtenauer Michael , Hähnel Valentin , Reiners David , Neuss Michael , Butter Christian 
Published: June 2021

Aims: To compare intermediate performance and mortality rates in patients, who underwent transcatheter aortic valve implantation (TAVI) with two different types of prostheses: Edwards Sapien 3 (ES3) and Direct Flow Medical (DFM).Methods and Results: 42 consecutive patients implanted with a DFM prosthesis for severe aortic stenosis were matched 1:1 with an equal number of patients, who received an ES3 during the same period. Primary endpoint was mortality. MACE, as a composite of all-cause death, stroke, and re-do-procedure (valve-in-valve), was defined as secondary endpoint. Moreover, we compared NYHA class, NT-proBNP-levels and the extent of restenosis. Patients were followed for 2 years. DFM patients showed echocardiographic elevated mean pressure gradients compared to ES3 patients before discharge (11.2 mmHg ± 5.3 vs. 3.5 mmHg ± 2.7; p &lt; 0.001) and upon 6-months follow-up (20.3 mmHg ± 8.8 vs. 12.3 mmHg ± 4.4; p &lt; 0.001). ES3 candidates showed superior NYHA class at follow-up (p = 0.001). Kaplan-Meier analysis revealed significantly worse survival in patients receiving a DFM prosthesis compared to ES3 (Breslow p = 0.020). MACE occurred more often in DFM patients compared to ES3 (Breslow p = 0.006).Conclusions: Patients receiving DFM valve prostheses showed worse survival and higher rates in MACE compared to ES3. Prosthesis performance regarding mean pressure gradients and patients' NYHA class also favored ES3.
Clinical and Functional Genetic Characterization of the Role of Cardiac Calcium Channel Variants in the Early Repolarization Syndrome
Authors: Chen Xiu , Barajas-Martínez Hector , Xia Hao , Zhang Zhonghe , Chen Ganxiao , Yang Bo , Jiang Hong , Antzelevitch Charles , Hu Dan 
Published: June 2021

Background: Early repolarization syndrome (ERS) is an inherited sudden cardiac death (SCD) syndrome. The present study investigates the role of genetic variants in cardiac calcium-channel genes in the pathogenesis of ERS and probes the underlying mechanisms.Methods: Polymerase chain reaction–based next-generation sequencing was carried out using a targeted gene approach. Unrelated ERS probands carrying calcium-channel variants were evaluated clinically and compared with matched healthy controls. Wild-type (WT) and mutant CACNA1C genes were coexpressed with CACNB2b and CACNA2D1 in HEK293 cells and studied using whole-cell patch-clamp techniques and confocal fluorescence microscope.Results: Among 104 ERS probands, 16 carried pathogenic variants in calcium-channel genes (32.2 ± 14.6 years old, 87.5% male). The symptoms at diagnosis included syncope (56.3%), ventricular tachycardia/fibrillation (62.5%), and SCD (56.3%). Three cases (18.8%) had a family history of SCD or syncope. Eight patients (50.0%) had a single calcium gene rare variant. The other half carried rare variants in other ERS-susceptible genes. Compared with controls, the heart rate was slower (72.7 ± 8.9 vs. 65.6 ± 16.1 beats/min, *p &lt; 0.05), QTc interval was shorter (408.2 ± 21.4 vs. 386.8 ± 16.9 ms, **p &lt; 0.01), and Tp-e/QT was longer (0.22 ± 0.05 vs. 0.28 ± 0.04, ***p &lt; 0.001) in single calcium mutation carriers. Electrophysiological analysis of one mutation, CACNA1C-P817S (c.2449C&gt;T), revealed that the density of whole-cell calcium current (ICa) was reduced by ~84.61% compared to WT (−3.17 ± 2.53 vs. −20.59 ± 3.60 pA/pF, n = 11 and 15, respectively, **p &lt; 0.01). Heterozygous expression of mutant channels was associated with a 51.35% reduction of ICa. Steady-state inactivation was shifted to more negative potentials and significantly accelerated as well. Confocal microscopy revealed trafficking impairment of CACNA1C-P817S (peripheral/central intensity: 0.94 ± 0.10 in WT vs. 0.33 ± 0.12 in P817S, n = 10 and 9, respectively, **p &lt; 0.01).Conclusions: ERS associated with loss-of-function (LOF) genetic defects in genes encoding the cardiac calcium channel represents a unique clinical entity characterized by decreased heart rate and QTc, as well as increased transmural dispersion of repolarization. In the case of CACNA1C-P817S, impaired trafficking of the channel to the membrane contributes to the LOF.
Genetic Variants in KIR/HLA-C Genes Are Associated With the Susceptibility to HCV Infection in a High-Risk Chinese Population
Authors: Shen Chao , Ge Zhijun , Dong Chen , Wang Chunhui , Shao Jianguo , Cai Weihua , Huang Peng , Fan Haozhi , Li Jun , Zhang Yun , Yue Ming 
Published: June 2021

BackgroundKIR/HLA-C signaling pathway influences the innate immune response which is the first defense to hepatitis C virus (HCV) infection. The aim of this study was to determine the association between the genetic polymorphisms of KIR/HLA-C genes and the outcomes of HCV infection in a high-risk Chinese population.MethodsIn this case-control study, four single nucleotide polymorphisms (SNPs) of KIR/HLA-C genes (KIR2DS4/KIR2DS1/KIR2DL1 rs35440472, HLA-C rs2308557, HLA-C rs1130838, and HLA-C rs2524094) were genotyped by TaqMan assay among drug users and hemodialysis (HD) patients including 1,378 uninfected control cases, 307 subjects with spontaneous viral clearance, and 217 patients with persistent HCV infection. Bioinformatics analysis was used to functionally annotate the SNPs.ResultsAfter logistic regression analysis, the rs35440472-A and rs1130838-A alleles were found to be associated with a significantly elevated risk of HCV infection (OR = 1.562, 95% CI: 1.229–1.987, P &lt; 0.001; OR = 2.134, 95% CI: 1.180–3.858, P = 0.012, respectively), which remained significant after Bonferroni correction (0.05/4). The combined effect of their risk alleles and risk genotypes (rs35440472-AA and rs1130838-AA) were linked to the increased risk of HCV infection in a locus-dosage manner (all Ptrend &lt; 0.001). Based on the SNPinfo web server, rs35440472 was predicted to be a transcription factor binding site (TFBS) while rs1130838 was predicted to have a splicing (ESE or ESS) function.ConclusionKIR2DS4/KIR2DS1/KIR2DL1 rs35440472-A and HLA-C rs1130838-A variants are associated with increased susceptibility to HCV infection in a high-risk Chinese population.
Lipoprotein-Associated Phospholipase A2: A Novel Contributor in Sjögren’s Syndrome-Related Lymphoma?
Authors: Nezos Adrianos , Skarlis Charalampos , Psarrou Anna , Markakis Konstantinos , Garantziotis Panagiotis , Papanikolaou Asimina , Gravani Fotini , Voulgarelis Michael , Tzioufas Athanasios G. , Koutsilieris Michael , Moutsopoulos Haralampos M. , Kotsifaki Eleni , Mavragani Clio P. 
Published: June 2021

BackgroundB-cell non-Hodgkin’s lymphoma (B-NHL) is one of the major complications of primary Sjögren’s syndrome (SS). Chronic inflammation and macrophages in SS minor salivary glands have been previously suggested as significant predictors for lymphoma development among SS patients. Lipoprotein-associated phospholipase A2 (Lp-PLA2)—a product mainly of tissue macrophages—is found in the circulation associated with lipoproteins and has been previously involved in cardiovascular, autoimmune, and malignant diseases, including lymphoma.ObjectiveThe purpose of the current study was to investigate the contributory role of Lp-PLA2 in B-NHL development in the setting of primary SS.MethodsLp-PLA2 activity in serum samples collected from 50 primary SS patients with no lymphoma (SS-nL), 9 primary SS patients with lymphoma (SS-L), and 42 healthy controls (HC) was determined by detection of [3H]PAF degradation products by liquid scintillation counter. Moreover, additional sera from 50 SS-nL, 28 SS-L, and 32 HC were tested for Lp-PLA2 activity using a commercially available ELISA kit. Lp-PLA2 mRNA, and protein expression in minor salivary gland (MSG) tissue samples derived from SS-nL, SS-L patients, and sicca controls (SC) were analyzed by real-time PCR, Western blot, and immunohistochemistry.ResultsSerum Lp-PLA2 activity was significantly increased in SS-L compared to both SS-nL and HC by two independent methods implemented [mean ± SD (nmol/min/ml): 62.0 ± 13.4 vs 47.6 ± 14.4 vs 50.7 ± 16.6, p-values: 0.003 and 0.04, respectively, and 19.4 ± 4.5 vs 15.2 ± 3.3 vs 14.5 ± 3.0, p-values: &lt;0.0001, in both comparisons]. ROC analysis revealed that the serum Lp-PLA2 activity measured either by radioimmunoassay or ELISA has the potential to distinguish between SS-L and SS-nL patients (area under the curve [AUC]: 0.8022, CI [95%]: 0.64–0.96, p-value: 0.004 for radioimmunoassay, and AUC: 0.7696, CI [95%]: 0.66–0.88, p-value: &lt;0.0001, for ELISA). Lp-PLA2 expression in MSG tissues was also increased in SS-L compared to SS-nL and SC at both mRNA and protein level. ROC analysis revealed that both MSG mRNA and protein Lp-PLA2 have the potential to distinguish between SS-nL and SS-L patients (area under the curve [AUC] values of 0.8490, CI [95%]: 0.71–0.99, p-value: 0.0019 and 0.9444, CI [95%]: 0.79–1.00, p- value: 0.0389 respectively). No significant difference in either serum Lp-PLA2 activity or MSG tissue expression was observed between SS-nL and HC.ConclusionsLp-PLA2 serum activity and MSG tissue mRNA/protein expression could be a new biomarker and possibly a novel therapeutic target for B-cell lymphoproliferation in the setting of SS.
Infiltration by CXCL10 Secreting Macrophages Is Associated With Antitumor Immunity and Response to Therapy in Ovarian Cancer Subtypes
Authors: Ardighieri Laura , Missale Francesco , Bugatti Mattia , Gatta Luisa Benerini , Pezzali Irene , Monti Matilde , Gottardi Stefano , Zanotti Laura , Bignotti Eliana , Ravaggi Antonella , Tognon Germana , Odicino Franco , Calza Stefano , Missolo-Koussou Yoann , Ries Carola Hermine , Helft Julie , Vermi William 
Published: June 2021

Ovarian carcinomas (OCs) are poorly immunogenic and immune checkpoint inhibitors (ICIs) have offered a modest benefit. In this study, high CD3+ T-cells and CD163+ tumor-associated macrophages (TAMs) densities identify a subgroup of immune infiltrated high-grade serous carcinomas (HGSCs) with better outcomes and superior response to platinum-based therapies. On the contrary, in most clear cell carcinomas (CCCs) showing poor prognosis and refractory to platinum, a high TAM density is associated with low T cell frequency. Immune infiltrated HGSC are characterized by the 30-genes signature (OC-IS30) covering immune activation and IFNγ polarization and predicting good prognosis (n = 312, TCGA). Immune infiltrated HGSC contain CXCL10 producing M1-type TAM (IRF1+pSTAT1Y701+) in close proximity to T-cells. A fraction of these M1-type TAM also co-expresses TREM2. M1-polarized TAM were barely detectable in T-cell poor CCC, but identifiable across various immunogenic human cancers. Single cell RNA sequencing data confirm the existence of a tumor-infiltrating CXCL10+IRF1+STAT1+ M1-type TAM overexpressing antigen processing and presentation gene programs. Overall, this study highlights the clinical relevance of the CXCL10+IRF1+STAT1+ macrophage subset as biomarker for intratumoral T-cell activation and therefore offers a new tool to select patients more likely to respond to T-cell or macrophage-targeted immunotherapies.
The Expression Profile and Prognostic Values of EPHA Family Members in Breast Cancer
Authors: Zhang Xixun 
Published: June 2021

BackgroundEphAs are a class of ephrin receptors that belong to the membrane-bound receptor tyrosine kinases group. Accumulating experimental evidence has shown that the EphA family is involved in tumor progression, namely in cell proliferation, invasiveness, and metastasis. EphAs are a promising target for anticancer therapy. However, their role in breast cancer (BC) is still not well understood.Materials and MethodsWe used a series of bioinformatic approaches to analyze the expression of the EphA family members and investigate their prognostic value in BC.ResultsLower expression levels of EphA2, EphA3, EphA4, EphA5, and EphA7 and higher expression levels of EphA10 were found in BC tissues compared to those in normal tissues. The expression levels of the EphA family genes were correlated with molecular subtyping but not with tumor stage. High expression levels of most EphAs indicated a better prognosis in BC.ConclusionsThis study suggested that EphA2, EphA3, EphA4, and EphA5 can act as tumor-inhibiting factors as well as biomarkers for the prognosis of BC.
The Neurogenic Compound P7C3 Regulates the Aerobic Glycolysis by Targeting Phosphoglycerate Kinase 1 in Glioma
Authors: Chen Wenjin , Jia Weiqiang , Wu Cuiying , Chen Lihua , Sun Kai , Wang Ji , Ding Boyun , Liu Ning , Xu Ruxiang 
Published: June 2021

BackgroundP7C3 is a neurogenic compound that exhibits neuroprotective properties in neural cells. However, its target proteins and effects in glioma are unknown.MethodsThe candidate P7C3 target proteins were analyzed using a human protein microarray containing 23136 human proteins. A streptavidin agarose affinity assay was used to verify the direct interaction between P7C3 and phosphoglycerate kinase 1 (PGK1). Mass spectrometry was used to identify the binding sites of PGK1 for P7C3 binding. Seahorse XF96 extracellular flux analyzer was used to measure the cell oxygen consumption rate and extracellular acidification rate. Glycolytic metabolites were measured using the related kits. Protein level was detected by western blotting and immunohistochemical staining. Autophagy was analyzed using a transmission electron microscope and western blotting. The malignancy of tumor progression in vitro and in vivo was analyzed based on cell viability, apoptosis and proliferation, migration and invasion, and xenograft model. Glial cells were marked by antibodies via immunohistochemical staining.ResultsThe human protein microarray identified 577 candidate P7C3 target proteins. The global profile of P7C3 target proteins indicated that P7C3 regulates glycolysis. Metabolic experiments confirmed that P7C3 regulates aerobic glycolysis in glioma cells. The underlying mechanism of P7C3 was found to be direct targeting PGK1 at lysine residues and asparagine residues, and the specific P7C3-PGK1 interaction led to decreased protein level and total intracellular kinase activity of PGK1. The Cancer Genome Atlas and Chinese Glioma Genome Atlas databases indicated that the mRNA level of PGK1 is significantly increased in high-grade glioma, and the abnormally high mRNA level of PGK1 is associated with a poor prognosis in patients with glioma, suggesting that PGK1 is a promising target for glioma therapy. The inhibition of PGK1 and the subsequent suppression of aerobic glycolysis caused by P7C3 inhibited the malignant growth of glioma in vitro and in vivo. Furthermore, P7C3 did not damage normal glial cells under concentration, which exhibit an inhibitory effect on gliomas.ConclusionsThis study revealed that P7C3 suppresses glioma by regulating aerobic glycolysis via directly targeting PGK1. Furthermore, we identified the P7C3 target proteins for the first time which is expected to provide scientific clues for future studies.