bluuebunny/crossref_metadata_2025_split huggingface dataset:
Socioeconomic inequality in mental well-being associated with COVID-19 containment measures in a low-incidence Asian globalized city
Authors: Chung Roger Yat-Nork , Chung Gary Ka-Ki , Chan Siu-Ming , Chan Yat-Hang , Wong Hung , Yeoh Eng Kiong , Allen Jessica , Woo Jean , Marmot Michael 
Published: November 2021

AbstractThe COVID-19 pandemic exposes and amplifies pre-existing inequalities even in places with relatively well-controlled outbreaks such as Hong Kong. This study aimed to explore whether the socioeconomically disadvantaged fare worse via various types of worry in terms of their mental health and well-being. Between September and October 2020, 1067 adults in Hong Kong were recruited via a cross-sectional population-wide telephone survey. The inter-relationship between deprivation, types of worry, mental health disorders, and subjective well-being was assessed using structural equation modelling. Results showed significant total effects of deprivation on worries about being infected (p = 0.002), economic activities and livelihood (p < 0.001), and personal savings (p < 0.001), as well as mental health disorders (p < 0.001) and subjective well-being (p < 0.001). Specifically, worry about economic activities and livelihood partly mediated the total effect of deprivation on mental health disorders (p = 0.004), whereas worry about personal savings and worry about economic activities and livelihood partially mediated the total effect of deprivation on subjective well-being (p = 0.007 and 0.002, respectively). Socioeconomic inequality, particularly in mental health and well-being, could be exacerbated via people’s economic concerns during the pandemic, which was largely induced by the COVID-19 containment measures rather than the pandemic per se given the relatively low COVID-19 incidence in Hong Kong.
Norovirus outbreaks in long-term care facilities in Catalonia from 2017 to 2018
Authors: Parrón Ignacio , Barrabeig Irene , Alseda Miquel , Rius Cristina , Cornejo-Sánchez Thais , Jané Mireia , Pérez Cristina , Guix Susana , Domínguez Àngela ,   the Working Group for the Study of Outbreaks of Acute Gastroenteritis in Catalonia, Pérez Cristina , Álvarez Josep , Barrabeig Irene , Sala Maria Rosa , Belver Anna Isabel , Rovira Ariadna , Parrón Ignacio , Coronas Lorena , Alsedà Miquel , Godoy Pere , de Andres Anna , de Benito Javier , Camprubí Esteve , Cunillé Montse , Lluïsa Forns M. , Moreno-Martínez Antonio , Razquín Efrén , Sabaté Sara , de Simón Mercé , Rius Cristina , Domínguez Àngela , Soldevila Núria , Bartolomé Rosa , Cornejo-Sánchez Thais , Jané Mireia , Martínez Ana , Torner Núria , Izquierdo Conchita , Vileu Rosa Maria , Guix Susana , Camps Neus , Sabaté Maria , Minguell Sofia , Carol Monica 
Published: December 2021

AbstractNorovirus is the leading cause of outbreaks of acute viral gastroenteritis. We carried out this study to investigate outbreaks in long-term care facilities reported in 2017 and 2018 in Catalonia (Spain). The characteristics of the centers, exposed persons and the genogroups responsible were analyzed. Viral loads were estimated. The attack rate (AR) of the outbreaks studied, and the rate ratio (RR) and the odds ratio (OR) and their 95% confidence intervals as measures of association were calculated. The mean cycle thresholds were compared using the t-test for independent means. We included 30 outbreaks (4631 exposed people). The global AR was 25.93%. The RR of residents vs. staff was 2.28 (95% CI 2.0–2.6). The RR between AR in residents with total or severe dependence vs. residents with moderate, low or no-dependence was 1.23 (95% CI 1.05–1.45). The AR were higher in smaller centers than in larger ones (38.47% vs. 19.25% and RR 2; 95% CI 1.82–2.2). GII was responsible for 70% of outbreaks. No association was found between the genogroup and presenting symptoms (OR 0.96; 95% CI 0.41–2.26). Viral loads were higher in symptomatic than in asymptomatic patients (p = 0.001).
A novel RNA-mediated mechanism causing down-regulation of insulating promoter interactions in human embryonic stem cells
Authors: Liu Yingjuan , Williams Simon G. , Jones Hayden R. , Keavney Bernard D. , Choy Mun-Kit 
Published: December 2021

AbstractThe genome-wide promoter interactome is primarily maintained and regulated by architectural proteins such as CTCF and cohesin. However, some studies suggest a role for non-coding RNAs (ncRNAs) in this process. We aimed to characterise the regulatory role of RNA-mediated promoter interactions in the control of gene expression. We integrated genome-wide datasets of RNA-chromatin and promoter-genome interactions in human embryonic stem cells (hESCs) to identify putative RNA-mediated promoter interactions. We discovered that CTCF sites were enriched in RNA-PIRs (promoter interacting regions co-localising with RNA-chromatin interaction sites) and genes interacting with RNA-PIRs containing CTCF sites showed higher expression levels. One of the long noncoding RNAs (lncRNAs) expressed in hESCs, Syntaxin 18-Antisense 1 (STX18-AS1), appeared to be involved in an insulating promoter interaction with the neighbouring gene, MSX1. By knocking down STX18-AS1, the MSX1 promoter-PIR interaction was intensified and the target gene (MSX1) expression was down-regulated. Conversely, reduced MSX1 promoter-PIR interactions, resulting from CRISPR-Cas9 deletion of the PIR, increased the expression of MSX1. We conclude that STX18-AS1 RNA antagonised local CTCF-mediated insulating promoter interactions to augment gene expression. Such down-regulation of the insulating promoter interactions by this novel mechanism may explain the higher expression of genes interacting with RNA-PIRs linked to CTCF sites.
Biodegradation and metabolic pathway of sulfamethoxazole by Sphingobacterium mizutaii
Authors: Song Jinlong , Hao Guijie , Liu Lu , Zhang Hongyu , Zhao Dongxue , Li Xingyang , Yang Zhen , Xu Jinhua , Ruan Zhiyong , Mu Yingchun 
Published: November 2021

AbstractSulfamethoxazole (SMX) is the most commonly used antibiotic in worldwide for inhibiting aquatic animal diseases. However, the residues of SMX are difficult to eliminate and may enter the food chain, leading to considerable threats on human health. The bacterial strain Sphingobacterium mizutaii LLE5 was isolated from activated sludge. This strain could utilize SMX as its sole carbon source and degrade it efficiently. Under optimal degradation conditions (30.8 °C, pH 7.2, and inoculum amount of 3.5 × 107 cfu/mL), S. mizutaii LLE5 could degrade 93.87% of 50 mg/L SMX within 7 days. Four intermediate products from the degradation of SMX were identified and a possible degradation pathway based on these findings was proposed. Furthermore, S. mizutaii LLE5 could also degrade other sulfonamides. This study is the first report on (1) degradation of SMX and other sulfonamides by S. mizutaii, (2) optimization of biodegradation conditions via response surface methodology, and (3) identification of sulfanilamide, 4-aminothiophenol, 5-amino-3-methylisoxazole, and aniline as metabolites in the degradation pathway of SMX in a microorganism. This strain might be useful for the bioremediation of SMX-contaminated environment.
Microplastics pollution in salt pans from the Maheshkhali Channel, Bangladesh
Authors: Rakib Md. Refat Jahan , Al Nahian Sultan , Alfonso María B. , Khandaker Mayeen Uddin , Enyoh Christian Ebere , Hamid Fauziah Shahul , Alsubaie Abdullah , Almalki Abdulraheem S. A. , Bradley D. A. , Mohafez Hamidreza , Islam Mohammad Aminul 
Published: November 2021

AbstractMicroplastics (MP) were recognized as an emergent pollution problem due to their ubiquitous nature and bioaccumulative potential. Those present in salt for consumption could represent a human exposure route through dietary uptake. The current study, conducted in Bangladesh, reports microplastics contamination in coarse salt prepared for human consumption. Sea salt samples were collected from eight representative salt pans located in the country's largest salt farming area, in the Maheshkhali Channel, along the Bay of Bengal. Microplastics were detected in all samples, with mean concentrations ranging from 78 ± 9.33 to 137 ± 21.70 particles kg−1, mostly white and ranging in size from 500–1000 µm. The prevalent types were: fragments (48%) > films (22%) > fibers (15%) > granules and lines (both 9%). Fourier transform mid-IR and near-IR spectra (FT-MIR-NIR) analysis registered terephthalate (48%), polypropylene (20%), polyethylene (17%), and polystyrene (15%) in all samples. These results contribute to the MP's pollution knowledge in sea salts to understand and reduce this significant human exposure route and environmental pollution source in the future.
Simulation of the impact of people mobility, vaccination rate, and virus variants on the evolution of Covid-19 outbreak in Italy
Authors: Spinella Corrado , Mio Antonio Massimiliano 
Published: December 2021

AbstractWe have further extended our compartmental model describing the spread of the infection in Italy. As in our previous work, the model assumes that the time evolution of the observable quantities (number of people still positive to the infection, hospitalized and fatalities cases, healed people, and total number of people that has contracted the infection) depends on average parameters, namely people diffusion coefficient, infection cross-section, and population density. The model provides information on the tight relationship between the variation of the reported infection cases and a well-defined observable physical quantity: the average number of people that lie within the daily displacement area of any single person. With respect to our previous paper, we have extended the analyses to several regions in Italy, characterized by different levels of restrictions and we have correlated them to the diffusion coefficient. Furthermore, the model now includes self-consistent evaluation of the reproduction index, effect of immunization due to vaccination, and potential impact of virus variants on the dynamical evolution of the outbreak. The model fits the epidemic data in Italy, and allows us to strictly relate the time evolution of the number of hospitalized cases and fatalities to the change of people mobility, vaccination rate, and appearance of an initial concentration of people positives for new variants of the virus.
Detection of Neisseria meningitidis in saliva and oropharyngeal samples from college students
Authors: Miellet Willem R. , Mariman Rob , Pluister Gerlinde , de Jong Lieke J. , Grift Ivo , Wijkstra Stijn , van Logchem Elske M. , van Veldhuizen Janieke , Immink Marie-Monique , Wijmenga-Monsuur Alienke J. , Rots Nynke Y. , Sanders Elisabeth A. M. , Bosch Thijs , Trzciński Krzysztof 
Published: November 2021

AbstractCarriage ofNeisseria meningitidisis an accepted endpoint in monitoring meningococcal vaccines effects. We have assessedN. meningitidisand vaccine-type genogroup carriage prevalence in college students at the time of MenACWY vaccine introduction in the Netherlands, and evaluated the feasibility of saliva sampling for the surveillance of carriage. For this, paired saliva and oropharyngeal samples collected from 299 students were cultured for meningococcus. The DNA extracted from all bacterial growth was subjected to qPCRs quantifying meningococcal and genogroup-specific genes presence. Samples negative by culture yet positive for qPCR were cultured again for meningococcus. Altogether 74 (25%) of students were identified as meningococcal carrier by any method. Sixty-one students (20%) were identified as carriers with qPCR. The difference between number of qPCR-positive oropharyngeal (n = 59) and saliva (n = 52) samples was not significant (McNemar’s test,p = 0.07). Meningococci were cultured from 72 students (24%), with a significantly higher (p < 0.001) number of oropharyngeal (n = 70) compared with saliva (n = 54) samples. The prevalence of genogroups A, B, C, W, and Y was none, 9%, 1%, 1% and 6%, respectively, and 8% of students carried MenACWY vaccine-type genogroup meningococci. Saliva is easy to collect and when combined with qPCR detection can be considered for meningococcal carriage studies.
An improved 2-level MPPT scheme for photovoltaic systems using a novel high-frequency learning based adjustable gain-MRAC controller
Authors: Sahu Pankaj , Dey Rajiv 
Published: November 2021

AbstractUnder rapidly changing environmental conditions, the model reference adaptive control (MRAC) based MPPT schemes need high adaptation gain to achieve fast convergence and guaranteed transient performance. The high adaptation gain causes high-frequency oscillations in the control signals resulting in numerical instability and inefficient operation. This paper proposes a novel high-frequency learning-based adjustable gain MRAC (HFLAG-MRAC) for a 2-level MPPT control architecture in photovoltaic (PV) systems to ensure maximum power delivery to the load under rapidly changing environmental conditions. In the proposed 2-level MPPT control architecture, the first level is the conventional ripple correlation control (RCC) that yields a steady-state ripple-free optimum duty cycle. The duty cycle obtained from the first level serves as an input to the proposed HFLAG-MRAC in the second level. In the proposed adaptive law, the adaptation gain varies as a function of the high-frequency ripple content of the tracking error. These high-frequency contents are the difference between the tracking error and its low-pass filtered version representing the fluctuations in output due to rapid changes in the environmental conditions. Thus, adjusting the adaptation gain by high-frequency content of the tracking error ensures fast convergence, guaranteed transient performance, and overall system stability without needing high adaptation gain. The adaptive law of the proposed HFLAG-MRAC is derived using the Lyapunov theory. Simulation studies, experimental analysis, and performance comparison with recent similar work validate the effectiveness of the proposed work.
Hidden biodiversity in microarthropods (Acari, Oribatida, Eremaeoidea, Caleremaeus)
Authors: Lienhard Andrea , Krisper Günther 
Published: November 2021

AbstractA challenge for taxonomists all over the world and across all taxonomic groups is recognizing and delimiting species, and cryptic species are even more challenging. However, an accurate identification is fundamental for all biological studies from ecology to conversation biology. We used a multidisciplinary approach including genetics as well as morphological and ecological data to assess if an easily recognizable, widely distributed and euryoecious mite taxon represents one and the same species. According to phylogenetic (based on mitochondrial and nuclear genes) and species delimitation analyses, five distinct putative species were detected and supported by high genetic distances. These genetic lineages correlate well with ecological data, and each species could be associated to its own (micro)habitat. Subsequently, slight morphological differences were found and provide additional evidence that five different species occur in Central and Southern Europe. The minuteness and the characteristic habitus ofCaleremaeus monilipestempted to neglect potential higher species diversity. This problem might concern several other “well-known” euryoecious microarthropods. Five new species of the genusCaleremaeusare described, namelyCaleremaeus mentobellussp. nov.,C. lignophilussp. nov.,C. alpinussp. nov.,C. elevatussp. nov., andC. hispanicussp. nov. Additionally, a morphological evaluation ofC. monilipesis presented.
Tidal variability of water quality parameters in a mesotidal estuary (Sado Estuary, Portugal)
Authors: Nascimento Ângela , Biguino Beatriz , Borges Carlos , Cereja Rui , Cruz Joana P. C. , Sousa Fátima , Dias Joaquim , Brotas Vanda , Palma Carla , Brito Ana C. 
Published: November 2021

AbstractTo establish effective water quality monitoring strategies in estuaries, it is imperative to identify and understand the main drivers for the variation of water quality parameters. The tidal effect is an important factor of the daily and fortnightly variability in several estuaries. However, the extent of that influence on the different physicochemical and biological parameters is still overlooked in some estuarine systems, such as the Sado Estuary, a mesotidal estuary located on the west coast of Portugal. The main objective of this study was to determine how the water quality parameters of the Sado Estuary varied with the fortnightly and the semidiurnal tidal variation. To achieve this goal, sampling campaigns were conducted in May/18, Nov/18 and Jun/19, under neap and spring tidal conditions, with data collection over the tidal cycle. Results were observed to be significantly influenced by the tidal variation, in a large area of the estuary. Flood seemed to mitigate possible effects of nutrient enrichment in the water column. Additionally, significant differences were also observed when considering the different sampling stations. Temperature, Suspended Particulate Matter (SPM) and nutrients showed the highest values at low water. Lastly, the implications of the tidal variability in the evaluation of the water quality according to Water Framework Directive were also discussed, highlighting the importance of studying short-time scale variations and the worst-case scenario to ensure water quality is maintained. These findings are relevant for the implementation of regional management plans and to promote sustainable development.
Combined transcriptome and metabolome integrated analysis of Acer mandshuricum to reveal candidate genes involved in anthocyanin accumulation
Authors: Zhang Shikai , Zhan Wang , Sun Anran , Xie Ying , Han Zhiming , Qu Xibin , Wang Jiayi , Zhang Laifu , Tian Mingshun , Pang Xuhong , Zhang Jinbao , Zhao Xiyang 
Published: November 2021

AbstractThe red color formation of Acer mandshuricum leaves is caused by the accumulation of anthocyanins primarily, but the molecular mechanism researches which underlie anthocyanin biosynthesis in A. mandshuricum were still lacking. Therefore, we combined the transcriptome and metabolome and analyzed the regulatory mechanism and accumulation pattern of anthocyanins in three different leaf color states. In our results, 26 anthocyanins were identified. Notably, the metabolite cyanidin 3-O-glucoside was found that significantly correlated with the color formation, was the predominant metabolite in anthocyanin biosynthesis of A. mandshuricum. By the way, two key structural genes ANS (Cluster-20561.86285) and BZ1 (Cluster-20561.99238) in anthocyanidin biosynthesis pathway were significantly up-regulated in RL, suggesting that they might enhance accumulation of cyanidin 3-O-glucoside which is their downstream metabolite, and contributed the red formation of A. mandshuricum leaves. Additionally, most TFs (e.g., MYBs, bZIPs and bHLHs) were detected differentially expressed in three leaf color stages that could participate in anthocyanin accumulation. This study sheds light on the anthocyanin molecular regulation of anthocyanidin biosynthesis and accumulation underlying the different leaf color change periods in A. mandshuricum, and it could provide basic theory and new insight for the leaf color related genetic improvement of A. mandshuricum.
Characterization of a G-quadruplex from hepatitis B virus and its stabilization by binding TMPyP4, BRACO19 and PhenDC3
Authors: Molnár Orsolya Réka , Végh András , Somkuti Judit , Smeller László 
Published: December 2021

AbstractSpecific guanine rich nucleic acid sequences can form non-canonical structures, like the four stranded G-quadruplex (GQ). We studied the GQ-forming sequence (named HepB) found in the genome of the hepatitis B virus. Fluorescence-, infrared- and CD-spectroscopy were used. HepB shows a hybrid form in presence of K+, but Na+, Li+, and Rb+ induce parallel structure. Higher concentrations of metal ions increase the unfolding temperature, which was explained by a short thermodynamic calculation. Temperature stability of the GQ structure was determined for all these ions. Na+ has stronger stabilizing effect on HepB than K+, which is highly unusual. The transition temperatures were 56.6, 53.8, 58.5 and 54.4 °C for Na+, K+, Li+, and Rb+ respectively. Binding constants for Na+ and K+ were 10.2 mM and 7.1 mM respectively. Study of three ligands designed in cancer research for GQ targeting (TMPyP4, BRACO19 and PhenDC3) showed unequivocally their binding to HepB. Binding was proven by the increased stability of the bound form. The stabilization was higher than 20 °C for TMPyP4 and PhenDC3, while it was considerably lower for BRACO19. These results might have medical importance in the fight against the hepatitis B virus.
Experimental investigation and modelling of a laboratory-scale latent heat storage with cylindrical PCM capsules
Authors: Jančík Petr , Schmirler Michal , Hyhlík Tomáš , Bláha Adam , Sláma Pavel , Devera Jakub , Kouba Jan 
Published: December 2021

AbstractHeat storage efficiency is required to maximize the potential of combined heat and power generation or renewable energy sources for heating. Using a phase change material (PCM) could be an attractive choice in several instances. Commercially available paraffin-based PCM was investigated using T-history method with sufficient agreement with the data from the manufacturer. The introduced LHTES with cylindrical capsules is simple and scalable in capacity, charging/discharging time, and temperature level. The overall stored energy density is 9% higher than the previously proposed design of similar design complexity. The discharging process of the designed latent heat thermal energy storage (LHTES) was evaluated for two different flow rates. The PCM inside the capsules and heat transfer fluid (HTF) temperature, as well as the HTF flow rate, were measured. The lumped parameter numerical model was developed and validated successfully. The advantage of the proposed model is its computational simplicity, and thus the possibility to use it in simulations of a whole heat distribution network. The so-called state of charge (SoC), which plays a crucial role in successful heat storage management, is a part of the evaluation of both experimental and computational data.
Impact of a nonnative parasitoid species on intraspecific interference and offspring sex ratio
Authors: Zhang Yao Zhuo , Jin Zhengya , Miksanek James Rudolph , Tuda Midori 
Published: December 2021

AbstractIn an assemblage of multiple predators sharing a single prey species, the combined effects of the component species may scale unpredictably because of emergent interspecific interactions. Prior studies suggest that chaotic but persistent community dynamics are induced by intra-/interspecific interactions between native and nonnative parasitoids competing over a shared host. Here, we test the impact of the nonnative parasitoid Heterospilus prosopidis (Hymenoptera: Braconidae) on the intraspecific interference and offspring sex ratio of the native parasitoid Anisopteromalus calandrae (Hymenoptera: Pteromalidae). We found that the nonnative parasitoid reduced intraspecific interference among native parasitoids and decreased the proportion of female offspring produced by the native parasitoid (predicted under conditions of reduced host availability). At higher host densities, the nonnative parasitoid contributed less to the total proportion of hosts parasitized, as its innate saturating Type II response changed to a dome-shaped Type IV response with increasing density of the native parasitoid, while the native parasitoid retained its increasing Type I response. This inverse host-density-dependent response between the two parasitoids and associated competitive superiority can explain the observed changes in parasitism; at high host densities, the searching efficiency of the native parasitoid increases via host feeding while the nonnative parasitoid experiences egg limitation. These results highlight the importance of the complementary top-down effects of multiple consumers on a single resource.