bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Effect of Seed Location on Functional Connectivity: Evidence from an Image-Based Meta-Analysis
Authors: Li Mengting , Sun Jiawei , Zhan Linlin , Lv Yating , Jia Xize , Ren Jun 
Published: December 2021

Abstract
Default mode network (DMN) is the most involved network in the study of brain development and brain diseases. Resting-state functional connectivity (rs-FC) is the most used method to study DMN, but different studies are inconsistent in the selection of seed. To evaluate the effect of different seed selection on rs-FC, we conducted an image-based meta-analysis (IBMA). We identified 59 coordinates of seed regions of interest (ROIs) within the default mode network (DMN) from 11 studies (retrieved from Web of Science and Pubmed) to calculate the functional connectivity; then, the uncorrected t maps were obtained from the statistical analyses. The IBMA was performed with the t maps. We demonstrate that the overlap of meta-analytic maps across different seeds’ ROIs within DMN is relatively low, which cautions us to be cautious with seeds’ selection. Future studies using the seed-based functional connectivity method should take the reproducibility of different seeds into account. The choice of seed may significantly affect the connectivity results.
The Effect of Thyroid Hormone Withdrawal, Performed to Evaluate the Success of I-131 Ablation on Quality of Life and Psychological Symptoms in Female Patients With Low-risk Differentiated Thyroid Cancer
Authors: Kupik Osman , Şen Bayram , Arpa Medeni , Akça Levent , Avcı Uğur , kupik Gülnihan eren , Gündoğdu Hasan , Kalcan Süleyman , Güçer Hasan , Akın Şafak , Tuncel Murat 
Published: December 2021

Abstract
Purpose: There is a need to evaluate the treatment response in patients who have undergone radioiodine treatment (RIT) for differentiated thyroid cancer. Our study aimed to show thyroid hormone withdrawal (THW) effects on quality of life and psychological symptoms in female patients with low-risk, well-differentiated papillary thyroid cancer. Methods: We applied the short form-36 (SF-36) and Symptom Checklist-90-R (SCL-90-R) questionnaires to the patients in the euthyroid state who have referred a median of 9 months (6-13 months) after RIT to perform a diagnostic whole-body scan (dWBS) and to evaluate stimulated Tg. We applied the same questionnaire again when thyroid-stimulating hormone (TSH) was > 30 μIU/mL 4 weeks after THW (hypothyroid state). We evaluated the changes in questionnaire scores using the paired-samples t-test or the Wilcoxon signed-rank test. Results: Our study included 52 patients (median age 48 years, range 23-65 years). There was a statistically significant worsening in anxiety, psychosis, additional items, and general symptom index symptoms with the SCL-90-R questionnaire, physical functioning, role limitation due to physical health, energy/fatigue, emotional well-being, social function, general health, and health change with the SF-36 questionnaire. Conclusions: THW worsened the patients' psychological symptoms and quality of life. Thyrotropin alfa is an alternative to reduce side effects, but it can be costly and difficult to obtain in developing countries. In order to reduce the side effects of hypothyroidism and avoid the high cost, treatment response assessment can be done only in selected patient groups.
Comparative Analysis of the Complete Mitochondrion of Chlorophytum Comosum with 9 Other Species
Authors: Cui Xueyu , Peng Yujiao , Hu Lin , Shao Yuanyuan , Song Enling , Hou Yanlin , Liu Shutian 
Published: December 2021

Abstract
Chlorophytum comosum belongs to genus Chlorophytum of family Asparagaceae that has more than 215 species. it is among 120 plant species assayed for phytoremediation of pollutants from indoor air. It has the ability to remove formaldehyde, nitrogen dioxide, carbon oxide, ozone, benzene, toluene, cigarette smoke and ammonia. It also is an important Chinese tradition medicine material. Aim to get more information about its mitochondrial genome, we sequenced the complete mitochondrial genome of C. comosum. The length of mitochondrial genome was 950316 bp with 36 protein coding genes, 5 rRNAs and 21 tRNAs. Although it has the biggest mitochondrial genome, the total PCGs content were the second least. C. comosum had the most repeats sequence and it may effect the size of mitochondrial genome, but base the result we found that the quantity of repeats sequence could not have the positive correlation with the mitochondrial size. Compared with other 9 species , we found in the mitochondrial genome of C. comosum, there were at least one PCGs suffered from the positive selection after divergence. Finally, the result of phylogenetic showed that C. comosum did not have colesd relationship with other species.
Mentha Piperita Extract as a Natural Product for the Corrosion Inhibition of Low Carbon Steel in a Polluted NaCl Environment: Chemical, Electrochemical and Biological Studies
Authors: Fouda Prof. Abd El-Aziz S. , Al-Bonayan Ameena M. , Eid Dalia M. 
Published: December 2021

Abstract
The impact of aqueous Mentha Piperita Extract (MPE) as a corrosion inhibitor for low carbon steel (LCS) in NaCl/Na2S solution was investigated using chemical and electrochemical techniques to evaluate the protection performance of MPE. “As the concentration of the extract increased, the protection efficiency (PE) increased, reaching 91.2% at 300 ppm, whereas the increase in temperature favored to slow decrease”. The extract was physically adsorbed on the metal surface according to the Temkin isotherm. Polarization data revealed that this extract acts as a mixed inhibitor. The mechanism of corrosion protection of MPE was discussed from electrochemical techniques. MPE was adsorbed on LCS obeying Temkin isotherm. Surface morphology techniques such as scanning electron microscopy (SEM) and atomic force microscopy (AFM) were used to demonstrate the creation of a protective coating on the LCS in the presence of MPE. The extract inhibits the bacterial activity of Escherichia coli.
WITHDRAWN: Endometriosis related sex steroid hormones, hypothalamic pituitary gonadal axis hormones and related animal modeling: A comprehensive review of published articles and endometriosis induction methods from 2010 to 2021 with scoring based approach
Authors: Salehpour Aria , Zare Afshin , Khoradmehr Arezoo , Mahdipour Mahdi , Tamadon Amin 
Published: December 2021

Abstract

Endometriosis is an enigmatic gynecological disease initiated by the ectopic growth of endometrial tissue and causes critical symptoms such as chronic pelvic pain, cyclic menstrual pain, subfertility or infertility. Considering extensive investigations for explaining the underlying pathophysiology of endometriosis, origin and distinctive processes which lead to endometritic state are not completely understood. In this comprehensive review, studies published from 2010 to 2021 are reviewed in order to provide a bright insight through the applications of translational animal models and endometriosis induction methods for evaluation of endometriosis pathogenesis and treatment. We provided method based inclusion criteria and reviewed all hormone-based studies with concentration on animal models. Additionally, studies with novel induction methods and approaches are categorized separately and analyzed by a novel scoring table for suitability of further investigations. Eventually, our scoring system suggested that the best-evaluated animal model for hormone related endometriosis studies is an “unopposed estrogenicity baboon model of endometriosis”.
Diagnostic value of systematic ultrasonography for trisomy 18 syndrome in fetuses before 16 weeks gestation
Authors: Zhou Jie , Dai Xiaohui , Liu Hanmin , Li Yiping , Luo Ling , Chen Jiao 
Published: December 2021

Abstract
Purpose

To explore the diagnostic value of systematic fetal ultrasonography for trisomy 18 (T18) syndrome before 16 weeks gestation.
Methods

A total of 12 fetuses with T18 were selected as research subjects and their nuchal translucency (NT) screening and fetal systematic ultrasonographic images acquired at 11–15 weeks were retrospectively analyzed.
Results

In the 12 fetuses’ NT screening, ten fetuses showed NT thickening, one showed nuchal cystic hygroma, four showed reversed a-wave ductus venosus flow, and three showed omphalocele. The most common anomalies on the systematic ultrasonography before 16 weeks gestation were cardiac defects (12/12, 100%), omphalocele (4/12, 33.3%), limb anomalies (5/12, 41.7%), and facial anomalies (3/12, 25.0%). Seven of the 12 fetuses had multiple structural malformations: three had two structural malformations (25.0%), three had three structural malformations (25.0%), and one had four structural malformations (8.3%).
Conclusion

Systematic fetal ultrasonography before 16 weeks gestation can detect most of the structural malformations of T18, effectively shortening the prenatal diagnosis time. It is therefore of great importance for reducing the birth rate of children with T18 and minimizing the physical and mental damage to mothers and their families.
Short-Term Outcomes of Laparoscopic/Robotic Gastrectomy Compared with Open Gastrectomy for Advanced Gastric Cancer Following Chemotherapy
Authors: Yoshikawa Kozo , Shimada Mitsuo , Tokunaga Takuya , Nakao Toshihiro , Nishi Masaaki , Takasu Chie , Kashihara Hideya , Wada Yuma , Yoshimoto Toshiaki , Yamashita Shoko 
Published: December 2021

Abstract
Purpose

This study aimed to investigate the short-term outcomes of laparoscopic gastrectomy/robotic gastrectomy after chemotherapy in patients with advanced gastric cancer and compare these outcomes with those of open gastrectomy.
Methods

Fifty patients who underwent radical gastrectomy for advanced gastric cancer after chemotherapy between 2007 and 2021 were retrospectively analyzed. The patients were divided into two groups: the laparoscopic gastrectomy/robotic gastrectomy (n = 11) and open gastrectomy (n = 39) groups. The short-term outcomes of these procedures were subsequently examined.
Results

The laparoscopic gastrectomy/robotic gastrectomy group had significantly shorter hospital stays and lower intraoperative blood loss than the open gastrectomy group. The overall complication rates were 12.8% (5 of 39 patients) and 0% (0 of 11 patients) in the open gastrectomy and laparoscopic gastrectomy/robotic gastrectomy groups, respectively (P = 0.1).
Conclusions

Laparoscopic gastrectomy/robotic gastrectomy may be a surgical option after chemotherapy for patients with advanced gastric cancer.
Neuron-Recognizable Characteristics of Peptides Recombined Using A Neuronal Binding Domain of Botulinum Neurotoxin
Authors: Kim Hye Rin , Jung Younghun , Shin Jonghyeok , Park Myungseo , Kweon Dae-Hyuk , Ban Choongjin 
Published: December 2021

Abstract
Recombinant peptides were designed using the C-terminal domain (receptor binding domain, RBD) and its subdomain (peptide A2) of a heavy chain of botulinum neurotoxin A-type 1 (BoNT/A1), which can bind to the luminal domain of synaptic vesicle glycoprotein 2C (SV2C-LD). Peptide A2- or RBD-containing recombinant peptides linked to an enhanced green fluorescence protein (EGFP) were prepared by expression in Escherichia coli. A pull-down assay using SV2C-LD-covered resins showed that the recombinant peptides for CDC5328 BoNT/A1, referred to EGFP-A2ʹ and EGFP-RBDʹ, exhibited ≥ 2.0-times stronger binding affinity to SV2C-LD than those for the wild-type BoNT/A1. Using bio-layer interferometry, an equilibrium dissociation rate constant (KD) of EGFP-RBDʹ to SV2C-LD was determined to be 5.45 mM, which is 33.87- and 15.67-times smaller than the KD values for EGFP and EGFP-A2ʹ, respectively. Based on confocal laser fluorescence micrometric analysis, the adsorption/absorption of EGFP-RBDʹ to/in differentiated PC-12 cells was 2.49- and 1.29-times faster than those of EGFP and EGFP-A2ʹ, respectively. Consequently, the recombinant peptides acquired reasonable neuron-specific binding/internalizing ability through the recruitment of RBDʹ. In conclusion, RBDs of BoNTs are versatile protein domains that can be used to mark neural systems and treat a range of disorders in neural systems.
G9a/GLP inhibition during ex vivo lymphocyte expansion increases in vivo cytotoxicity of engineered TCR-T cells against hepatocellular carcinoma
Authors: Lam Maxine , Reales-Calderon Jose , Ow Jin Rong , AW Joey , TAN Damien , Ceccarello Erica , TABAGLIO TOMMASO , Lim Yan Ting , Wang Loo Chien , Sobota Radoslaw , Adriani Giulia , Bertoletti Antonio , Guccione Ernesto , PAVESI Andrea 
Published: December 2021

Abstract
Engineered T cells transiently expressing tumor-targeting receptors are an attractive form of engineered T cell therapy as they carry no risk of insertional mutagenesis or long-term adverse side-effects. However, multiple rounds of treatment are often required, increasing patient discomfort and cost. To mitigate this, we sought to improve the antitumor activity of transient engineered T cells by screening a panel of small molecules targeting epigenetic regulators for their effect on T cell cytotoxicity. Using a model for engineered T cells targetting hepatocellular carcinoma, we found that short-term inhibition of G9a/GLP increased T cell antitumor activity in in vitro models and an orthotopic mouse model. G9a/GLP inhibition increased granzyme expression without terminal T cell differentiation or exhaustion and resulted in specific changes in expression of genes and proteins involved in pro-inflammatory pathways, T cell activation and cytotoxicity.
Body Composition, Dietary Intake and Biochemical Profile in Thalassemia Major Patients, Southwest of Iran
Authors: Koor Behrooz Ebrahimzadeh , Yousefi Mojtaba , Mohammadian Zahra , Etemadfar Peyman 
Published: December 2021

Abstract
Background: Thalassemia, as an autosomal recessive disorder, leads to life-threatening anemia, in which patient's life depends on long term blood transfusion and regular medical care. In this study, body composition, nutritional intake and biochemical parameters of blood transfusing dependent major thalassemia patients were evaluated.Methods: In this cross-sectional study, 74 major thalassemia volunteers referred to educational hospital in Yasuj city were recruited. Body composition, demographic and biochemical parameters were measured. Data were statistically analyzed using parametric and non-parametric statistical tests. P-values less than 0.05 were defined as statistically significant.Results: Data analysis showed that mean of age of participants in this cross-sectional study was 21.09± 7.7 years old. 21.6% of our patients were underweight, 68.9% of them were normal weight, 9.5% were overweight and none of them were obese. 8.1% of < 20 years old patients were short stature. About 80% of patients had low blood HDL concentration. About 30% of participants were at borderline levels ​and 42% of patients were at high and toxic levels of Ferritin. Approximately, all patients had hemoglobin lower than standard of healthy individuals. The mean body fat percent of patients was 24 ± 6.6%, which was higher than the normal value ​​(P <0.001), with exception of copper, phosphorus and vitamin K and percent of protein and carbohydrates from total energy were significantly lower than standard values. The Binary logistic regression analysis also showed that the interval between sessions of blood transfusion (P=0.03), daily intake of vitamin D (P=0.01) and vitamin K (P=0.028) and fatness (0.006) were significant predictors of the Ferritin equation as iron overload in thalassemia patients.Discussion and Conclusion: Malnutrition, especially underweight was evident among our patients. Intake of the energy and most nutrients were lower than standard values. Biochemical parameters such as Ferritin and hemoglobin were also abnormal. Blood levels of vitamin D in thalassemia patients should be routinely monitored. Clinical trials on the effect of supplementation with nutrients such as vitamin D and calcium and zinc are recommended. Blood levels of vitamin D in thalassemia patients should be routinely monitored every six months.
Identification of a Cancer Stemness Driven Prognostic Model and Therapeutic Drug Targets by Machine Learning in Hepatocellular Carcinoma
Authors: Lai Mingchun , Xi Bin , Wei Shenyu , Zhang Wenjin , Zheng Shusen 
Published: December 2021

Abstract
Background: Hepatocellular carcinoma (HCC) is one of the most common malignancies. Cancer stem cells (CSCs), characterized by self-renewal and drug-resistance, play an important role in the development and progression of diverse cancers, but the underlying association of HCC and CSCs is not fully researched.Methods: Transcriptome and clinical data of 903 patients in four independent HCC cohorts were obtained from TCGA, ICGC, and GEO databases. We evaluated the stemlike index for each patient to reflect the cancer stemness by using one-class logistic regression (OCLR) algorithm. GISTIC 2.0, Maftools and GSVA were used to reveal the association between the stemness index and genomic variation and biological processes in HCC. The differential expression analysis, univariate Cox analysis and LASSO analysis were used to identify the prognostic stemness signatures. The HCC stemness-related risk score (HCSRS) was constructed to quantify stemness levels of individual tumors. Based on HCSRS, the nomogram was established for HCC prognosis in a quantitative approach. Additionally, single sample Gene Set Enrichment Analysis (ssGSEA) algorithm was used to evaluate the immune infiltration levels in HCC, and drug response analysis was adopted to identify potential agents with drug sensitivity in high-HCSRS score patients.Results: The stemness index in HCC tissues was significantly higher than that in normal tissues, and there was a significant positive correlation with pathological grade. Patients with high stemness index showed higher somatic mutation frequency, tumor mutation load, and copy number variation frequency, and were significantly enriched in tumor-related signaling pathways. Meanwhile, the 7-gene based HCSRS model that was trained and validated in 4 independent cohorts exhibited high predictive significance for overall survival (OS). Further analysis revealed that patients with high HCSRS possessed higher immunosuppression status, characterized by significantly decreased infiltration of anti-tumor immune cells (CD8 T cells, cytotoxic T cells, DC cells, NK cells, etc.) and exhausted CYT responses. At last, a total of twelve agents were identified to have potential therapeutic effects in high-HCSRS patients.Conclusion: In current study, we systematically analyzed the potential relationship of HCC stemness with genomic variation, tumor microenvironment and biological processes, provided a theoretical basis for individualized treatment of HCC patients.
A Novel Alternately Energy Source Strategy for Re-Ablation of Patients with Recurrent Atrial Fibrillation—Cryoballoon or Radiofrequency Current Energy Ablation. A Case-Control Study
Authors: Tang Qinghui , Guo XiaoGang , Ma Jian 
Published: December 2021

Abstract
Background: Atrial fibrillation recurrence after circumferential pulmonary vein (PV) isolation was common. Which ablation technique is better for repeat ablation in patients with recurrent atrial fibrillation (AF) remains unclear. We aimed to investigate long-term efficacy of repeat ablation using a novel alternately energy source sequence for re-ablation of patients with recurrent atrial fibrillation: cryoballoon (CB) re-ablation for patients with a failed radiofrequency (RFC) ablation (RFC-CB redo group); radiofrequency energy re- ablation for patients with a failed cryoballoon ablation (CB-RFC redo group).Method: Recurrent AF patients received a repeat ablation procedure in our hospital were enrolled into the study. Demographic and re-ablation procedural characteristics and outcomes were compared among groups.Results: A total of 156 patients were enrolled into the study, 60 patients (38.5%) were in the CB-RFC-redo group and 96 patients (61.5%) were in the RFC-CB-redo group. Longer duration of AF (69.31±64.69 vs 50.78 ±51.48 months; P=0.039) and longer time from first ablation to re-ablation (54.02 ±38.10 vs 14.2 ±10.5 months; P=0.001) were observed in the RFC-CB-redo group as compared with the CB-RFC redo group. Early recurrence rates of atrial fibrillation following initial ablation were equal among groups (RFC-CB-redo group: 42.7% vs CB-RFC-redo group:48.3% , p=0.515).The number of reconnected PVs was significantly higher in the RFC-CB redo group than the CB-RFC redo group (3.36 ± 0.96 vs 1.50 ± 0.81, p=0.01). During the average follow-up of 10.7 ±2.41 months, significantly less AF recurrence was observed in the CB-RFC redo group (16.7% vs 31.3%, p=0.045). In the multivariate analysis, different energy ablation sequence, AF type and early recurrence after the initial ablation were independent predictors of AF recurrence after re-ablation.Conclusions: Alternate energy source sequence strategy for re-ablation of patients with recurrent atrial fibrillation was safe and moderately effective. However, Large multi-center studies must be warranted to provide conclusive evidence.
Postoperative Morbidity Risk Factors After Conservative Surgery of Hydatic Cyst of the Liver: A Retrospective Study of 151 Hydatic Cysts of the Liver
Authors: Ghannouchi Mossaab , Rodayna Hawas , khlifa Mohamed Ben , nacef karim , boudokhan moez 
Published: December 2021

Abstract
Background: Hepatic hydatid cyst is an endemic parasitosis in Tunisia. Although most of these cysts are benign; their treatment may lead to several complications which can cause an important post-operative morbidity and mortality.The purpose of the present paper is to assess the morbidity and mortality specifics risk factors of hepatic hydatid cysts after conservative surgery.Methods: We conducted a retrospective study of 102 patients over a period of 13 years, from 2006 to 2019. We included all patients operated on for hydatid cyst of the liver, complicated and uncomplicated, in the Department of General Surgery of Mahdia, Tunisia.We excluded patients who received an exclusive medical treatment and those who have other hydatic cyst localizations.Descriptive statistics,bivariate analysis using chi-squared test and Fisher’s exact test for categorical variables;t-test,ANOVA , and Kruskal–Wallis for continuous variables; odds ratio calculations, ordinal and multivariate logistic regression models were appliedResults: The cohort was composed of 102 patients with a total of 151 cysts operated on using conservative surgery, among them there was 75 women (73, 5%) and 27 men (26.5%). The median age was 43, with extremes ranging from 12 to 88 years. The majority of patients (94. 1%) were from rural areas. The cysts were uncomplicated in about half of the cases (48%), elsewhere complications such as compression of neighboring organs (25,5%), opening in the bile ducts (16,7%), infection (9,8%), and rupture in the peritoneum (2%) were found. Conservative surgery was the mainstay of treatment with an overall mortality rate of 1. 9%. The overall morbidity rate was 22%: 14% specific morbidity and 8% non-specific morbidity. External biliary fistula was the most common postoperative complication (9%). The predictive factors of morbidity in univariate analysis were Preoperative hydatid cyst infection (P = 0.01), Compressive cysts (P=0.05), preoperative fever and jaundice, (respectively P=0. 03 and P=0. 02), no one achieved statistical significance in the multivariate modelConclusions:Preoperative hydatid cyst infection, compressive cysts and preoperative fever and jaundice are significant predictor factors of morbidity after conservative surgery for liver hydatid cyst. They must be considered in the treatment and the surgical decision for patients with hydatid cyst.