bluuebunny/crossref_metadata_2025_split huggingface dataset:
Antibody response to two doses of inactivated SARS‐CoV‐2 vaccine (CoronaVac) in kidney transplant recipients
Authors: Eren Sadioğlu Rezzan , Demir Erol , Evren Ebru , Aktar Merve , Şafak Seda , Artan Ayşe Serra , Meşe Sevim , Ağaçfidan Ali , Çınar Güle , Önel Mustafa , Karahan Zeynep Ceren , Şengül Şule , Keven Kenan , Türkmen Aydın 
Published: October 2021

AbstractBackgroundCoronavirus Disease‐19 (COVID‐19) has high mortality in kidney transplant recipients (KTR), and vaccination against severe acute respiratory syndrome‐coronavirus‐2 (SARS‐CoV‐2) is vital for this population. Although the humoral response to messenger RNA vaccines was shown to be impaired in KTR, there is a lack of data regarding the antibody response to inactivated vaccines. We investigated the antibody response to two consequent doses of the inactivated SARS‐CoV‐2 vaccine (CoronaVac; Sinovac Biotech, China).MethodsA total of 118 patients from two centers were included. The levels of anti‐SARS‐CoV‐2 immunoglobulin‐G antibodies against the nucleocapsid and spike antigens were determined with enzyme immunoassay (DIA.PRO; Milano, Italy) before the vaccine and one month after the second dose of the vaccine. Thirty‐three patients were excluded due to antibody positivity in the serum samples obtained before vaccination.ResultsEighty‐five patients, 47 of whom were female, with a mean age of 46 ± 12, were included in the statistical analysis. The maintenance immunosuppressive therapy comprised tacrolimus (88.2%), mycophenolate (63.6%), and low‐dose steroids (95.3%) in the majority of the patients. After a median of 31 days following the second dose of the vaccine, only 16 (18.8%) patients developed an antibody response. The median (IQR) antibody level was 52.5 IU/ml (21.5–96). Age (48 vs. 38, p = .005) and serum creatinine levels (1.14 vs. 0.91, p = .04) were higher in non‐responders and were also found to be independently associated with the antibody response (odds ratio (OR): 0.93, p = 0.012 and 0.15, p = 0.045, respectively) in multivariate analysis.ConclusionIn this study, we found the antibody response to the inactivated vaccine to be considerably low (18.8%) in KTR. Increased age and impaired renal function were associated with worse antibody response. Based on the knowledge that mRNA vaccines yield better humoral responses, this special population might be considered for additional doses of mRNA vaccination.
The activity of labile coagulation factors and fibrinogen in thawed plasma during a 5 day storage period in the hospital blood bank refrigerator
Authors: Bahreini Sahar , Javadzadeh Shahshahani Hayedeh , Nasizadeh Soheila , Ahmadinejad Minoo 
Published: October 2021

AbstractBackgroundFresh frozen plasma (FFP) is used to treat coagulation disorders. Even though the activity of labile coagulation factors gradually decreases once thawed, it can be used up to 24 h after thawing, if stored properly. In this study, the level of coagulation factor activity was evaluated in thawed plasma during a 5 day storage period.Materials and methodsThis cross‐sectional study was performed on 40 FFP units prepared in Yazd Blood Center. Samples were thawed in a waterbath for 20–30 min at 30–37°C and then stored in the hospital blood bank refrigerator. The level of fibrinogen concentration, as a stable factor and, coagulation factors V and VIII, as labile factors, were measured in the plasma immediately following the thawing process as well as 24 and 120 h after the process. Data analysis was performed using SPSS software 20.ResultsThe fibrinogen level remained stable for up to 24 h after thawing; after 120 h there was a 1.66% decrease with the mean level of 334.0 ± 53.3 mg/dl. The mean activity of factors V and VIII levels decreased by 12.3%, and 26% respectively over 120 h after thawing when compared to that after 24 h. A 120 h after thawing Factor V activity was above 70% in 87.5% of thawed plasmas and its mean activity was 81.6 ± 11.8. Factor VIII activity was above 70% in only 35% of thawed plasmas with the mean activity of 64.4 ± 17.2.ConclusionThawed plasma can be used for up to 5 days in all therapeutic applications of FFP since it still has the essential hemostatic effects. However, in situations where higher levels of FVIII are needed, Thawed Plasma is not a suitable alternative. In such cases FFP, FVIII concentrate or cryoprecipitated antihemophilic factor should be used.
Formative Development of a Technology-Based Physical Activity Intervention for Grandparents and Grandchildren
Authors: Kobayashi Marissa A. , St. George Sara M. , Leite Rafael O. , Noriega Esquives Blanca , Wetstone Rachel , Pulgaron Elizabeth R. , Prado Guillermo , Czaja Sara J. 
Published: October 2021

Physical inactivity is a major public health issue among older adults and children. This study presents preliminary results that will inform the development of a technology-based physical activity intervention for grandparents and grandchildren (ages 6–12 years old). The authors used an iterative user-centered design framework to gather quantitative data from grandparents (n = 35) and subsequently invited a subset of 12 of them to engage in qualitative interviews. Participants were 63.1 ± 9.8 years old, 80% female, 64% U.S.-born, 43% Hispanic, 66% single, and 40% <$15K income. The majority of grandparents reported mobile device proficiency, very close relationships with their grandchildren, and interest in participating in an intergenerational intervention. Four key themes related to family closeness, dynamics, routines, and technology informed intervention development. Next steps involved a pilot trial using Fitbits and a fully functioning technology-based prototype. Grandparents are uniquely positioned within their families to serve as agents of change in health-promoting interventions.
Influence of Chlorides of Mono- and Divalent Metals on the Oligomeric Composition of Lysozyme Crystallization Solutions and Further Crystal Growth
Authors: Marchenkova M. A. , Konarev P. V. , Boikova A. S. , Ilina K. B. , Pisarevsky Yu. V. , Kovalchuk M. V. 
Published: October 2021

Abstract
The influence of the precipitant type (LiCl, NaCl, KCl, NiCl2, and CuCl2) on the formation of oligomers (dimers and octamers) in lysozyme crystallization solutions at two protein concentrations has been investigated by small-angle X-ray scattering (SAXS). The same solutions have been used to grow crystals in order to reveal the influence of the oligomeric composition on the crystal growth. The data obtained in this and previous studies on the influence of precipitant concentration yield an inversely proportional dependence of the total content of octamers and dimers on the cation atomic number, which is in agreement with the increase in the ion activity in the lyotropic series for Li+, Na+, and K+ and the increase in the ionic radius for Li+, Na+, K+, Ni2+, and Cu2+. It is shown that a decrease in the protein concentration in a crystallization solution leads to a decrease in octamer volume fraction at an invariable volume fraction of dimers and reduces the probability of crystal formation.
Prenatal ultrasonographic markers for prediction of complex gastroschisis and adverse perinatal outcomes: a systematic review and meta-analysis
Authors: Sun Raphael C , Hessami Kamran , Krispin Eyal , Pammi Mohan , Mostafaei Shayan , Joyeux Luc , Deprest Jan , Keswani Sundeep , Lee Timothy C , King Alice , Belfort Michael A , Shamshirsaz Alireza A 
Published: October 2021

ObjectiveWe sought to perform a meta-analysis of the predictive value of antenatal ultrasonographic markers of bowel dilation, gastric dilation, polyhydramnios and abdominal circumference that predict complex gastroschisis and adverse perinatal outcomesData sourcesPubMed, Web of Science, Scopus and Embase were searched for relevant articles up to December 2020. Studies reporting prenatal ultrasonographic markers including intra-abdominal bowel dilation (IABD), extra-abdominal bowel dilation (EABD), bowel wall thickness, polyhydramnios, abdominal circumference <5th percentile, gastric dilation (GD) and bowel dilation not otherwise specified (BD-NOS) were included. The primary outcome was prediction of complex gastroschisis; secondary outcomes were length of hospital stay for newborn, time to full enteral feeding, postnatal mortality rate, incidence of necrotising enterocolitis and short bowel syndrome.ResultsThirty-six studies were included in this meta-analysis. We found significant associations between complex gastroschisis and IABD (OR=5.42; 95% CI 3.24 to 9.06), EABD (OR=2.27; 95% CI 1.40 to 3.66), BD-NOS (OR=6.27; 95% CI 1.97 to 19.97), GD (OR=1.88; 95% CI 1.22 to 2.92) and polyhydramnios (OR=6.93; 95% CI 3.39 to 14.18). Second trimester IABD and EABD have greater specificity for the prediction of complex gastroschisis than third trimester values with specificity of 95.6% (95% CI 58.1 to 99.7) and 94.6% (95% CI 86.7 to 97.9) for the second trimester IABD and EABD, respectively.ConclusionPrenatal ultrasonographic markers, especially the second trimester IABD and EABD, can identify fetuses that develop complex gastroschisis. Furthermore, these specific ultrasonographic markers can identify those babies at the highest risk for severe complications of this congenital anomaly and hence selected for future antenatal interventions.
Management of limbal stem cell deficiency by amnion-assisted conjunctival epithelial redirection using vacuum-dried amniotic membrane and fibrin glue
Authors: Dua Harminder Singh , Ting Darren Shu Jeng , AlSaadi Ahmed , Said Dalia G 
Published: October 2021

PurposeTo study the outcome of a modified amnion-assisted conjunctival epithelial redirection (ACER) technique using vacuum-dried amnion (Omnigen) and fibrin glue for managing total limbal stem cell deficiency (LSCD).MethodA retrospective, interventional case series of all patients with total LSCD who underwent limbal stem cell transplant (LSCT) using the modified ACER procedure between 2016 and 2019. The outcome was defined as: (1) success: complete corneal re-epithelialisation without conjunctivalisation; (2) partial success: sub-total corneal re-epithelialisation with partial non-progressive conjunctivalisation sparing the visual axis and (3) failure: conjunctivalisation affecting the visual axis.ResultsTen patients (six men), with a mean age of 46.2±18.4 years, were included. The mean follow-up was 23.0±13.9 months. Causes of LSCD were chemical eye injury (30%), congenital aniridia-related keratopathy (30%), ocular surface malignancy (20%), Steven-Johnson syndrome (10%) and contact lens overuse (10%). 50% were bilateral. The time from diagnosis to ACER (for acquired causes) was 45.6±44.4 months. 80% of patients achieved a complete/partial success following ACER and 20% of patients required repeat LSCT. Auto-LSCT was associated with a significantly higher chance of success than allo-LSCT (p=0.048). The mean best-corrected-visual-acuity (logMAR) improved significantly from 1.76±0.64 preoperatively to 0.94±0.94 at final follow-up (p=0.009). Omnigen was available off-the-shelf stored at room temperature and its transparency enabled visualisation of the healing epithelium beneath.ConclusionLSCT using the modified ACER serves as an effective ocular surface reconstruction technique in managing total LSCD and improving vision. Vacuum-dried amnion provides advantages of easy handling, transparency and storage at room temperature.
Detecting plaque rupture? Soluble CD40 ligand for early diagnosis of acute myocardial infarction in the emergency department
Authors: Body Richard 
Published: October 2021

BackgroundIn recent years there has been growing interest in the identification of novel biomarkers that may enable early exclusion of acute coronary syndromes (ACS) in the Emergency Department (ED). Soluble CD40 ligand (sCD40L) has been identified as a potential marker of coronary plaque destabilisation and platelet activation. We aimed to investigate the value of sCD40L measured at the time of ED presentation for enabling early diagnosis and exclusion of ACS.MethodsWe recruited patients presenting to the ED with suspected cardiac chest pain within the previous 24 h. We measured sCD40L in plasma samples taken at the point of ED presentation. All patients had troponin T measured at least 12 h after symptom onset and were followed up for 6 months for adverse events (death, acute myocardial infarction (AMI) or the need for urgent revascularisation).Results706 patients were recruited to the study. sCD40L levels were significantly lower in smokers, in patients with hyperlipidaemia or prior coronary revascularisation and in patients taking statins, r angiotensin-converting enzyme or calcium blockers. There was no significant difference in sCD40L levels between patients with (median 33.8ng/ml, IQR 18.0–80.5) and without (36.3 ng/ml, IQR 14.2–94.3) AMI (p=0.92) or between patients who did (34.0 ng/ml, 14.2–94.3) and who did not (34.9, 18.2–80.4) develop adverse events (p=0.82). The area under the Receiver Operating Characteristic curve was 0.50 (95% CI 0.45 to 0.56) for AMI and 0.49 (0.44–0.55) for adverse events. There was no trend towards increasing incidence of either outcome with ascending quintiles of sCD40L.ConclusionssCD40L is unhelpful in the ED diagnosis of ACS. Future work in this area should focus on other biomarkers.
The ABC of handover: a new tool for handover in the emergency department and its impact on practice
Authors: Farhan Maisse 
Published: October 2021

ObjectivesA two-part study identifying best practice for shift handover and introducing a new handover tool; the ABC of Handover. We evaluate its impact and explore user opinions. The ABC tool aims to standardise handover providing effective transition of responsibility in the E.D; enhancing patient safety.MethodsPart 1 consensus based study; structured interviews identified current practice of handover. Best practice was identified and the ABC mnemonic was developed to classify information. Part 2: prospective observational before-and-after study exploring the effect of The ABC of Handover on clinical and organisational practice; measured by observing handovers and verifying the information transmitted by comparing to actual events occurring during the shift (providing a score). Observations were repeated after implementation of The ABC of Handover. Finally, questionnaire were used to appraise the tool.ResultsInterviews revealed lack of standardised practice and identified best practice including clinical and operational strategies to organise the next shift. The ABC method was developed which grouped “best practice” under those headings.41 handovers were observed before implementation and 42 after. Each handover was given a score according to the number of items it contained that were necessary to that particular shift. The mean score given to handovers pre-implementation was 34% with a modal value of 50%. Using The ABC of Handover the mean score was 86% with a modal value of 100%. All participants reported ease of use and improvement with the ABC tool.ConclusionsThis study demonstrates disparity in the understanding of shift handover and identifies best practice in the ABC of Handover which standardised and improved handover. It was successfully implemented and led to a change of practice and a global improvement, providing a framework for safe transition of shift responsibility. This can be used for training in handover for medical and nursing staff in the ED.
Neuroimaging Risk Factors for Participation Restriction after Acute Ischemic Stroke: 1-Year Follow-up Study
Authors: Qu Jian-Feng , Zhong Huo-Hua , Liang Wen-Cong , Chen Yang-Kun , Liu Yong-Lin , Li Wei 
Published: October 2021

The aim of the present study was to determine the neuroimaging predictors of poor participation after acute ischemic stroke. A total of 443 patients who had acute ischemic stroke were assessed. At 1-year recovery, the Reintegration to Normal Living Index was used to assess participation restriction. We also assessed the Activities of Daily Living Scale and modified Rankin Scale (mRS) score. Brain MRI measurement included acute infarcts and pre-existing abnormalities such as enlarged perivascular spaces, white matter lesions, ventricular-brain ratio, and medial temporal lobe atrophy (MTLA). The study included 324 men (73.1%) and 119 women (26.9%). In the univariate analysis, patients with poor participation after 1 year were older, more likely to be men, had higher National Institutes of Health Stroke Scale (NIHSS) score on admission, with more histories of hypertension and atrial fibrillation, larger infarct volume, more severely enlarged perivascular spaces and MTLA, and more severe periventricular hyperintensities and deep white matter hyperintensities. Patients with participation restriction also had poor activities of daily living (ADL) and mRS score. Multiple logistic regression showed that, in model 1, age, male gender, NIHSS score on admission, and ADL on follow-up were significant predictors of poor participation, accounting for 60.2% of the variance. In model 2, which included both clinical and MRI variables, male gender, NIHSS score on admission, ADL on follow-up, and MTLA were significant predictors of poor participation, accounting for 61.2% of the variance. Participation restriction was common after acute ischemic stroke despite good mRS score. Male gender, stroke severity, severity of ADL on follow-up, and MTLA may be predictors of poor participation. Trial registration number ChiCTR1800016665.
Residency interviews in the digital era
Authors: Beshar Isabel , Tate William J , Bernstein Dan 
Published: October 2021

AbstractIn the midst of the SARS-CoV-2 pandemic, the US Association of American Medical Colleges (AAMC) required residency programme transition from in-person to virtual interviews for all applicants. The new virtual format upended a system that has relied on programmes and applicants balancing the likelihood of acceptance with the financial and time demands of cross-country travel.In this commentary, we address the history of residency interviewing in the USA and the emerging changes that are taking place in light of virtual interviews. We discuss the advantages of the new online format, including the reduced cost for applicants and programmes, as well as the decreased carbon footprint.We also discuss the inequities of virtual interviewing, involving a national maldistribution of interviews to only the top-tier candidates. We share previously unpublished data on the number of virtual interviews accepted by Stanford's 2020 residency applicants, compared with those conducted in person in 2019. We find Stanford applicants in all fields accepted more interviews: from a mean of 8 in 2019 to 14 in 2020, a change of 160% on average. Despite this, only half of Stanford 2020 applicants interviewing in the virtual format thought they had accepted more interviews than they would have in person.We comment on how transitions to online interviewing may be affecting medical schools and applicants disproportionately. Ultimately, we highlight the need and offer ideas for additional regulation on behalf of the AAMC to ensure a more equitable distribution of interview opportunities.
The changing retail landscape for tobacco: dollar stores and the availability of cheap cigarettes among tobacco-related priority populations
Authors: Raskind Ilana G , Vishwakarma Monika , Schleicher Nina C , Andersen-Rodgers Elizabeth , Henriksen Lisa 
Published: October 2021

IntroductionDollar stores are rapidly altering the retail landscape for tobacco. Two of the three largest chains sell tobacco products in more than 24 000 stores across the USA. We sought to examine whether dollar stores are more likely to be located in disadvantaged neighbourhoods and whether dollar stores charge less for cigarettes than other tobacco retailers.MethodsData were collected from a statewide random sample of licensed tobacco retailers in California (n=7678) in 2019. Logistic regression modelled odds of a census tract containing at least one dollar store as a function of tract demographics. Linear mixed models compared price of the cheapest cigarette pack by store type, controlling for tract demographics.ResultsCensus tracts with lower median household income, rural status and higher proportions of school-age youth were more likely to contain at least one dollar store. The cheapest cigarette pack cost less in dollar stores compared with all store types examined except tobacco shops. Estimated price differences ranged from $0.32 (95% CI: 0.14 to 0.51) more in liquor stores and $0.39 (95% CI: 0.22 to 0.57) more in convenience stores, to $0.82 (95% CI: 0.64 to 1.01) more in small markets and $1.86 (95% CI: 1.61 to 2.11) more in stores classified as ‘other’.ConclusionsDollar stores may exacerbate smoking-related inequities by contributing to the availability of cheaper cigarettes in neighbourhoods that are lower income, rural and have greater proportions of youth. Pro-equity retail policies, such as minimum price laws and density reduction policies, could mitigate the health consequences of dollar stores’ rapid expansion.
Relationship of critical speed derived from a 10-min submaximal treadmill test to 5-km and 10-km running performances
Authors: Follador Lucio , de Borba Edilson Fernando , da Silva Sergio Gregorio 
Published: October 2021

It has been shown that the critical speed (CS) predicted from a perceptually self-regulated 10-min submaximal treadmill test (T10) is reliable and closely matches the CS estimated from conventional methods. To assess the relationship between the T10 and 5-km and 10-km running performances, 36 recreational runners (mean SD: age: 32.2 ± 6.2 years, height: 173.2 ± 7.3 cm, weight: 70.9 ± 8.8 kg, maximal oxygen uptake (V̇O2max): 53.3 ± 6.1 mL·kg−1·min−1) performed a ramp incremental test and 2 T10 tests (the first as a familiarization trial). Results showed that the T10 CS (3.9 ± 0.44 m·s−1) was significantly correlated with runners’ last 6 months best performances in 5 km (20.3 ± 2.7 min; r = –0.90) and 10 km (42.7 ± 5.7 min; r = –0.91), the V̇O2max (r = 0.75), the speed associated with the gas exchange threshold (vGET: 3.38 ± 0.36 m·s−1; r = 0.76), the speed associated with the second ventilatory threshold (vVT2: 4.15 ± 0.49 m·s−1; r = 0.84), and the speed associated with the V̇O2max (vV̇O2max: 4.78 ± 0.54 m·s−1; r = 0.87). Moreover, 79% and 83% of the variance in 5-km and 10-km performances could be explained solely by the CS predicted from the T10. Results evidenced the strong relationship and practical performance relevance of the T10 CS test. Novelty: Critical speed derived from a 10-min submaximal treadmill test (T10) is significantly correlated with 5-km and 10-km running performances. The T10 critical speed test may represent a useful tool for assessing running performance capabilities.