bluuebunny/crossref_metadata_2025_split huggingface dataset:
A familiar study on self-limited childhood epilepsy patients using hIPSC-derived neurons shows a bias towards immaturity at the morphological, electrophysiological and gene expression levels
Authors: Casalia Mariana L. , Casabona Juan Cruz , García Corina , Cavaliere Candedo Verónica , Quintá Héctor Ramiro , Farías María Isabel , Gonzalez Joaquín , Gonzalez Morón Dolores , Córdoba Marta , Consalvo Damian , Mostoslavsky Gustavo , Urbano Francisco J. , Pasquini Juana , Murer Mario Gustavo , Rela Lorena , Kauffman Marcelo A. , Pitossi Fernando J. 
Published: November 2021

AbstractBackgroundSelf-limited Childhood Epilepsies are the most prevalent epileptic syndrome in children. Its pathogenesis is unknown. In this disease, symptoms resolve spontaneously in approximately 50% of patients when maturity is reached, prompting to a maturation problem. The purpose of this study was to understand the molecular bases of this disease by generating and analyzing induced pluripotent stem cell-derived neurons from a family with 7 siblings, among whom 4 suffer from this disease.MethodsTwo affected siblings and, as controls, a healthy sister and the unaffected mother of the family were studied. Using exome sequencing, a homozygous variant in the FYVE, RhoGEF and PH Domain Containing 6 gene was identified in the patients as a putative genetic factor that could contribute to the development of this familial disorder. After informed consent was signed, skin biopsies from the 4 individuals were collected, fibroblasts were derived and reprogrammed and neurons were generated and characterized by markers and electrophysiology. Morphological, electrophysiological and gene expression analyses were performed on these neurons.ResultsBona fide induced pluripotent stem cells and derived neurons could be generated in all cases. Overall, there were no major shifts in neuronal marker expression among patient and control-derived neurons. Compared to two familial controls, neurons from patients showed shorter axonal length, a dramatic reduction in synapsin-1 levels and cytoskeleton disorganization. In addition, neurons from patients developed a lower action potential threshold with time of in vitro differentiation and the amount of current needed to elicit an action potential (rheobase) was smaller in cells recorded from NE derived from patients at 12 weeks of differentiation when compared with shorter times in culture. These results indicate an increased excitability in patient cells that emerges with the time in culture. Finally, functional genomic analysis showed a biased towards immaturity in patient-derived neurons.ConclusionsWe are reporting the first in vitro model of self-limited childhood epilepsy, providing the cellular bases for future in-depth studies to understand its pathogenesis. Our results show patient-specific neuronal features reflecting immaturity, in resonance with the course of the disease and previous imaging studies.
Biofilm formation and antagonistic activity of Lacticaseibacillus rhamnosus (PTCC1712) and Lactiplantibacillus plantarum (PTCC1745)
Authors: Rezaei Zeinab , Khanzadi Saeid , Salari Amir 
Published: November 2021

AbstractCurrently, the health benefits of probiotic bacteria are well known, and this has taken up a great deal of space in food science and health, both research and operational. On the other hand, anti-biofilm properties on food pathogens in the food and pharmaceutical industries have created an attractive challenge. This study aimed to describe the inhibitory activity of cell-free supernatants (CFS), planktonic cells, and biofilm form of lactobacilus strains (L. rhamnosus and L. plantarum) against food pathogens such as Pseudomonas aeruginosa and Listeria monocytogenes. Anti-bacterial activities of the CFS of lactobacillus strains were assessed by the microplate method and via violet staining. Evaluation of the antagonistic activity of planktonic cells and biofilm of LAB were performed by the spread plate method. The results showed the incubation time of 48 h was the best time to produce biofilm. Although the planktonic states reduce the pathogens bacterial about 1 –1.5 log, but in biofilm forms, decreased L. monocytogenes about 4.5 log compared to the control, and in the case of P. aeruginosa, a growth reduction of about 2.13 log was observed. Furthermore, biofilm formation of L. monocytogenes in the presence of L. rhamnosus cell-free supernatant was more weakly than L. plantarum CFS, but their CFS effect on reducing the bacterial population of P. aeruginosa was the same. According to the study, biofilm produced by probiotic strains can be considered a new approach for biological control. Also, cell-free supernatant can be used as postbiotic in the food and pharmaceutical industries.
The effects of nebulized ketamine and intravenous magnesium sulfate on corticosteroid resistant asthma exacerbation; a randomized clinical trial
Authors: Farshadfar Kimia , Sohooli Maryam , Shekouhi Ramin , Taherinya Ali , Qorbani Mostafa , Rezaei-kojani Mehdi 
Published: November 2021

Abstract
Background and aims
Asthma exacerbation is defined as an acute attack of shortness of breath with more than 25% decrease in morning peak flow compared to the baseline on 2 consecutive days, which requires immediate standard therapy. The majority of asthmatic patients are considered to be steroid-sensitive; however, corticosteroid-resistant asthma is a subset of asthma with poor response to corticosteroids and is responsible for frequent hospital admissions. In this study we aimed to compare the effects of two enhancing strategies, the nebulized ketamine and IV magnesium sulfate, in treatment of severe steroid resistant asthma.

Materials and methods
This double-blind randomized clinical trial was conducted on patients who presented to a referral clinic in Alborz, Iran. Using random allocation, patients were divided into two groups. The first group was treated with nebulized ketamine and the second group was treated with intravenous magnesium sulfate. Peak expiratory flow rates were assessed before the intervention, 30 and 60 min after the intervention and compared with the aid of SPSS software.

Results
The Peak expiratory flow rates before the intervention, 30 min and 60 min after the intervention was statistically significantly different in both ketamine and magnesium sulfate groups. Peak expiratory flow rates change between 0 and 60 min were 29.4 and 15.2% in the ketamine and magnesium sulfate group respectively. Although the ketamine group showed much higher increase in mean PEFR compared to the MgSO4 groups, there was no statistically significant difference across both groups.

Conclusion
Our study concluded that combined with standard therapy, both ketamine and IV magnesium sulfate are effective agents in the improvement of PEFR in patients with acute severe asthma that failed to respond to traditional therapies. However, there were no statistically significant difference between the two groups.
Identifying relations between posture and pain in lower back pain patients: a narrative review
Authors: Kripa Sai , Kaur Harmanpreet 
Published: November 2021

AbstractPosture is a “body’s attitude or the positioning of the limbs when standing or sitting.” There are many examples of different postures which include lordotic posture, swayback posture, flat back, and anterior pelvic tilt. Everyone in some of the other parts of their life considers pain as an unpleasant feeling or sensation that is experienced. Specifically, low back pain can be relentless and daunting to many people who often recovered without the need of a health care professional or any treatment by changing their posture or performing the movement. Various factors are leading to low back pain other than the postural fault, for instance, age, sedentary lifestyle, anxiety, and sleep. However, for back pain, posture has become deep-rooted in people’s thoughts. Health care professionals have instructed people to correct their posture to fight back pain. As people become older, the posture becomes worse, but this does not appear to cause pain. If any person cannot move his/her body, that does not mean that they are having a problem involving posture; that is actually because of a problem relating to movement. A study proved that there is no difference in the lordotic angle of the populace having an issue with LBP. There is a decrease in the range and speed of the movement performed. What matters is the movement rather than the appearance of standing or sitting. The primary aim of this paper is to improve knowledge and understanding of the association between posture and LBP, as, speaking about recent researches, they have observed no association between posture and LBP. There are many studies published to support this evidence. People call the importance of posture and alignment for while performing a heavy deadlift, land a jump, or any strenuous activity. Therefore, an urge to write a paper on this topic is to change the mindset of many people worrying out there about their postural faults or their appearances by providing information about varying their static posture to conform to some ideal and keep moving to improve their function.
Global Risk Indicator and Therapy for Older Patients With Diffuse Large B-Cell Lymphoma: A Population-Based Study
Authors: Di Mengyang , Keeney Tamra , Belanger Emmanuelle , Panagiotou Orestis A. , Olszewski Adam J. 
Published: November 2021

PURPOSE: To examine the impact of global risk, a measure comprising age, comorbidities, function, and cognitive statuses, on treatment selection and outcomes among older home care recipients with diffuse large B-cell lymphoma. METHODS: From SEER-Medicare, we selected home care recipients diagnosed with diffuse large B-cell lymphoma in 2011-2015, who had pretreatment Outcome and Assessment Information Set (OASIS) evaluations. We created a global risk indicator categorizing patients as low-, moderate-, or high-risk on the basis of OASIS assessments. We examined the association of global risk with receipt of therapy and among chemotherapy recipients, with mortality, emergency department visits, hospitalization, and intensive care unit admission within 30 days from first treatment in logistic models, reporting adjusted odds ratios (OR) with 95% CI. We compared overall survival across risk groups estimating adjusted hazard ratios. RESULTS: Of the 1,232 patients (median age, 80 years), 65% received chemotherapy. High-risk patients ( v moderate-risk) were less likely to receive any chemotherapy (OR, 0.50; 95% CI, 0.39 to 0.64) and curative regimens (OR, 0.59; 95% CI, 0.40 to 0.86) if treated, although even in the moderate-risk group, only 61% received curative regimens. High-risk patients were more likely to experience acute mortality (OR, 2.24; 95% CI, 1.43 to 3.52), emergency department visits (OR, 1.35; 95% CI, 1.00 to 1.83), hospitalization (OR, 1.60; 95% CI, 1.19 to 2.17), or intensive care unit admission (OR, 1.52; 95% CI, 1.04 to 2.22) and had inferior overall survival (hazard ratio, 1.41; 95% CI, 1.11 to 1.78). CONCLUSION: Global risk on the basis of OASIS is easily available, suggesting a potential way to improve patient selection for curative treatment and institution of preventive measures.
Experiences of Health Insurance among American Indian Elders and Their Health Care Providers
Authors: Jaramillo Elise Trott , Haozous Emily A. , Willging Cathleen E. 
Published: November 2021

Abstract
Context: American Indian elders have a lower life expectancy than other aging populations in the United States because of inequities in health and in access to health care. To reduce such disparities, the 2010 Affordable Care Act included provisions to increase insurance enrollment among American Indians. Although the Indian Health Service remains underfunded, increases in insured rates have had significant impacts among American Indians and their health care providers.
Methods: From June 2016 to March 2017, we conducted qualitative interviews with 96 American Indian elders (age 55+) and 47 professionals (including health care providers, outreach workers, public-sector administrators, and tribal leaders) in two southwestern states. Interviews focused on elders' experiences with health care and health insurance. We analyzed transcripts iteratively using open and focused coding techniques.
Findings: Although tribal health programs have benefitted from insurance payments, the complexities of selecting, qualifying for, and maintaining health insurance are often profoundly alienating and destabilizing for American Indian elders and communities.
Conclusions: Findings underscore the inadequacy of health-system reforms based on the expansion of private and individual insurance plans in ameliorating health disparities among American Indian elders. Policy makers must not neglect their responsibility to directly fund health care for American Indians.
Mapping the metabolomic and lipidomic changes in the bleomycin model of pulmonary fibrosis in young and aged mice
Authors: Weckerle Jelena , Picart-Armada Sergio , Klee Stephan , Bretschneider Tom , Luippold Andreas H. , Rist Wolfgang , Haslinger Christian , Schlüter Holger , Thomas Matthew J. , Krawczyk Bartlomiej , Fernandez-Albert Francesc , Kästle Marc , Veyel Daniel 
Published: November 2021

ABSTRACTAlterations in metabolic pathways were recently recognized as potential underlying drivers of idiopathic pulmonary fibrosis (IPF), translating into novel therapeutic targets. However, knowledge of metabolic and lipid regulation in fibrotic lungs is limited. To comprehensively characterize metabolic perturbations in the bleomycin mouse model of IPF, we analyzed the metabolome and lipidome by mass spectrometry. We identified increased tissue turnover and repair, evident by enhanced breakdown of proteins, nucleic acids and lipids and extracellular matrix turnover. Energy production was upregulated, including glycolysis, the tricarboxylic acid cycle, glutaminolysis, lactate production and fatty acid oxidation. Higher eicosanoid synthesis indicated inflammatory processes. Because the risk of IPF increases with age, we investigated how age influences metabolomic and lipidomic changes in the bleomycin-induced pulmonary fibrosis model. Surprisingly, except for cytidine, we did not detect any significantly differential metabolites or lipids between old and young bleomycin-treated lungs. Together, we identified metabolomic and lipidomic changes in fibrosis that reflect higher energy demand, proliferation, tissue remodeling, collagen deposition and inflammation, which might serve to improve diagnostic and therapeutic options for fibrotic lung diseases in the future.
Time Is Relative: How Framing of Time Estimation Affects the Accuracy of Cost Information
Authors: Schuhmacher Karl , Burkert Michael 
Published: November 2021

Accurate cost information is critical to effective decision making within organizations. Cost computations often rely on subjective judgments by employees regarding the amount of time that different tasks consume. In an experimental setting, we examine the accuracy of two common approaches to eliciting subjective time estimates vital for accurate cost information. Specifically, we compare estimation error when employees estimate (i) the total time for all iterations of a task (the pool approach) versus (ii) the average time for one iteration of a task (the unit approach). These two approaches have received interest by both practitioners and researchers and are at the heart of the difference between conventional activity-based costing (ABC) and time-driven ABC. While mathematically equivalent, we hypothesize and find that the two approaches evoke different cognitive processes that lead to differences in estimation error. Relative to the unit approach, the pool approach produces larger error in the allocation of time among different tasks, but only when the number of iterations per task varies across tasks. Further, the pool approach results in overestimation of productive time, whereas the unit approach leads to underestimation of productive time. Our findings are robust to different response modes of the pool approach (estimates in absolute time units and in percentages). This study is relevant for designers and users of cost and performance-measurement systems in that allocation errors lead to cost cross-subsidization and poor resource-allocation decisions, while overall errors undermine capacity utilization decisions. This paper was accepted by Brian Bushee, accounting.
Report: A Comprehensive Student Support Program in Mental Health // برنامج دعم طلابي شامل للصحة النفسية
Authors: Sherein Abdeen  , Samah Jabr  , Michael Morse  , Katherine Lyman  , Elizabeth Berger  
Published: November 2021

In response to an identified need to improve the emotional resilience of children in Palestine, the Palestinian Medical Education Initiative (PMED) developed a comprehensive program to support student mental health that was delivered at a private school in East Jerusalem in 2015–2016. This report describes the structure and function of the comprehensive program, in which training was provided for all school staff and for parents in a series of meetings and workshops, in addition to the establishment of a permanent school-based Taskforce, led by the trainer, consisting of two teachers, the principal, and the guidance counselor. The report also outlines the processes of outcomes measurement and evaluation of this program.
The aim of the Taskforce was to develop school-wide programming supporting student resilience and to make specific plans to address individual students who had been identified with behavioral or learning problems. The success of this pilot program demonstrates the effectiveness of the training/Taskforce model and suggests that future policy planning and research in Palestine consider adopting similar comprehensive student support programs.
0.1–5  MHz ultrahigh-speed gas density distributions using digital holographic interferometry
Authors: Rodrigues Neil S. , Brown Alex D. , Meyer Terrence R. , Lucht Robert P. 
Published: November 2021

Gas density distributions for an underexpanded jet at several different pressure ratios were measured at ultrahigh speeds in this work using digital holographic interferometry (DHI). DHI measurements have generally been performed on the order of several Hz in the literature, although some recent groups report measurements at 10 and 100 kHz. We demonstrate 2D imaging of gas density distributions at imaging rates up to 5 MHz, which is an increase by a factor of 50 compared to the previous DHI literature. A narrow-linewidth, continuous-wave laser was used in a Mach–Zehnder configuration, and the holograms were recorded using one of two different CMOS cameras. The interferograms were analyzed using the Fourier method, and a phase unwrapping was performed. Axisymmetric flow was assumed for the region near the nozzle exit, and an Abel inversion was performed to generate a planar-slice gas density distribution from the line-of-sight unwrapped phase. The challenges and opportunities associated with performing DHI measurements at ultrahigh speeds are discussed.
Optimization of coating uniformity in a simple rotation system by using
a rotating shadow mask
Authors: Long Guoyun , Zhang Yaoping , Fan Junqi , Guan Chunlin 
Published: November 2021

A theoretically designed rotating shadow mask is proposed to optimize the uniformity of a simple rotation system, which makes full use of the width of the coating chamber. This method can fabricate a large-aperture optical component, the diameter of which is more than half the width of the coating machine. The rotating shadow mask is applied to correct the film thickness uniformity near the center point of simple plane substrate. The factors influencing the effect of the rotating shadow mask are simulated and discussed. Then the shape of the rotating shadow mask is theoretically designed, and the uniformity within a corresponding radius is well corrected. After determining the shape of the rotating shadow mask, an additional fixed shadow mask is calculated and used to improve the uniformity of the entire substrate. Through the application of the two shadow masks together, uniformity about 99.5% is obtained in the diameter of 640 mm on a 1100 mm coating machine.
Compact and broadband silicon-based polarization beam splitter using asymmetric directional couplers embedded with subwavelength gratings and slots
Authors: Dai Sijie , Xiao Jinbiao 
Published: November 2021

A compact and broadband silicon-based polarization beam splitter (PBS) is proposed and investigated in detail, where the two arms of the directional coupler (DC) are, respectively, embedded with subwavelength gratings (SWGs) and vertical slots so that field distributions for the TE mode are significantly changed, effectively weakening coupling strength, whereas those for the TM mode are almost unaffected, nearly analogous to the DC with strip waveguides. By carefully optimizing structural parameters, efficient coupling will emerge between the two waveguides for the TM mode, while TE mode will be confined in the SWG-assisted strip waveguide. Consequently, the two modes can be effectively separated, and thus the realization of a PBS is accomplished. Results show that a compact PBS with a coupling length of 6.45 µm is achieved, together with the extinction ratio (ER) of 27.54/31.88 dB, the insertion loss of 0.12/0.14 dB, and the reflection loss of

−

43.67

/

−

30.50

d
B

, respectively, for TE/TM mode at the wavelength of 1.55 µm. The bandwidth, for both modes, is up to 230/100 nm when ER is larger than 15/20 dB. In addition, fabrication tolerances to the critical structural parameters and field evolution through the proposed device are analyzed.
Risk and protective factors of neurocognitive disorders in older adults in Central and Eastern Europe: A systematic review of population-based studies
Authors: Wolfova Katrin , Kucera Matej , Cermakova Pavla 
Published: November 2021

Background
A wide range of potentially modifiable risk factors, indicating that the onset of neurocognitive disorders can be delayed or prevented, have been identified. The region of Central and Eastern Europe has cultural, political and economic specifics that may influence the occurrence of risk factors and their link to the cognitive health of the population.

Objective
We aimed to systematically review population-based studies from Central and Eastern Europe to gather evidence on risk and protective factors for neurocognitive disorders.

Methods
We searched the electronic databases PubMed, Cochrane Database of Systematic Reviews, PsycINFO, Web of Science, and Embase. The search was performed on 26th of February 2020 and repeated at the end of the review process on 20th May 2021.

Results
We included 25 papers in a narrative synthesis of the evidence describing cardiovascular risk factors (n = 7), social factors (n = 5), oxidative stress (n = 2), vitamins (n = 2), genetic factors (n = 2) and other areas (n = 7). We found that there was a good body of evidence on the association between neurocognitive disorders and the history of cardiovascular disease while there were gaps in research of genetic and social risk factors.

Conclusion
We conclude that the epidemiological evidence from this region is insufficient and population-based prospectively followed cohorts should be established to allow the development of preventive strategies at national levels.