bluuebunny/crossref_metadata_2025_split huggingface dataset:
Exogenously applied nitrogenous fertilizers and effective microorganisms improve plant growth of stevia (Stevia rebaudiana Bertoni) and soil fertility
Authors: Youssef Mohamed Ahmed , Yousef Ahmed Fathy , Ali Muhammad Moaaz , Ahmed Alshaymaa I. , Lamlom Sobhi F. , Strobel Wacław Roman , Kalaji Hazem M. 
Published: September 2021

AbstractThe effects of different fertilizers and biofertilizers on crop production to increase plant growth, improve quality and yield components (dry leaves yield, leaf protein, and stevioside) of crops has been extensively studied. However, the combination of both types of fertilizers have rarely been investigated. To explore the effect of different fertilizers and biofertilizers on stevia plant, a two-year field experiment was conducted to investigate the growth response of stevia plants under the influence of nitrogenous fertilizers (NFs) and effective microorganisms (EM). The experiment was laid out in a split-plot design, with EM as the main plot factor (−EM and +EM) and NFs as the subplot factor [control, chemical NFs (Ch-N) and organic NFs (Org-N)]. The results showed that, plants treated with EM and Org-N showed 2-, 2.2-, 2.4-, 2.5-, 3.3- and 3-fold increases in plant height, number of branches, total leaf area, plant fresh weight, plant dry weight and leaf dry yield, respectively, compared to untreated plants. Similarly, plants receiving EM along with Ch-N showed 1.86-, 1.7-, 2.2-, 2.12-, 3-, and 2.72-fold increases in the same traits. Total chlorophyll, protein, N, P, K and sativoside contents were increased by 88.8, 152, 138, 151.5, 43 and 137.5% when EM and Org-N were applied to stevia plants. Application of EM together with Ch-N increased these properties by 0.5, 127.7, 115, 216, 42.6 and 83.8%, respectively in the same traits. Overall, the combined application of NFs and EM improved growth, yield and nutrient accumulation in stevia plants.
Book review of The Wandering Herd: The Medieval Cattle Economy of South East England c. 450-1450 by Andrew Margetts
Authors: Kerr Brian 
Published: September 2021

Book detailsMargetts, A. The Wandering Herd: The Medieval Cattle Economy of South East England c. 450-1450. Windgather Press, 2021. 272 pages, ISBN 978-1-91118-879-7
Tulasnella calospora (UAMH 9824) retains its effectiveness at facilitating orchid symbiotic germination in vitro after two decades of subculturing
Authors: Zettler Lawrence W. , Dvorak Caleb J. 
Published: September 2021

Abstract
Background
The technique of symbiotic germination—using mycorrhizal fungi to propagate orchids from seed in vitro—has been used as one method to cultivate orchids in North America and abroad for > 30 years. A long-held assumption is that mycorrhizal fungi used for this purpose lose their effectiveness at germinating seeds over time with repeated subculturing.

Results
We provide evidence for the lingering efficacy of one particular strain of Tulasnella calospora (266; UAMH 9824) to stimulate seed germination exemplified by the North American terrestrial orchid, Spiranthes cernua, as a case study. This fungus was originally acquired from roots from Spiranthes brevilabris in 1999 and sub-cultured during the two decades since. Seeds inoculated with the fungus in vitro developed to an advanced protocorm stage after 16 days, and leaf elongation was pronounced after 42 days. In a pilot study, seedlings co-cultured with Tulasnella calospora 266 were deflasked after 331 days and later transferred to soil under greenhouse conditions where they eventually initiated anthesis. During the course of two decades, seeds of 39 orchid species, cultivars and hybrids spanning 21 genera, germinated in vitro co-cultured with Tulasnella calospora 266. These orchids included temperate terrestrials and tropical epiphytes alike.

Conclusions
The sustained effectiveness of this fungus is noteworthy because it argues against the concept of mycorrhizal fungi losing their symbiotic capability through prolonged subculturing. This study serves as an example of why in situ habitat preservation is essential for the conservation of orchids as a source of potentially useful mycorrhizal fungi.
Improvement of clinical and radiological outcomes of root repair patients with advanced articular cartilage degeneration and osteoarthritis
Authors: Hopkins Nathan D. M. , Lawrie Steven 
Published: September 2021

Abstract
Purpose

The main purpose of this study was to investigate clinical and radiological outcomes of medial meniscus posterior root tear (MMPRT) repair in knees with advanced articular cartilage degeneration and osteoarthritis compared to those with minimal degenerative change.

Methods
Thirty-three knees underwent MMPRT repair using an arthroscopic pullout repair tibial tunnel technique. Clinical scores including Lysholm Score, International Knee Documentation Committee (IKDC) Score and Knee injury and Osteoarthritis Outcome (KOOS) Score were collected preoperatively and sequentially at 6 months, 12 months and mean final follow-up of 39.4 months. Kellgren-Lawrence (K-L) osteoarthritis grade, Outerbridge classification of cartilage degeneration and the presence of bone marrow oedema on MRI were also evaluated.

Results
All clinical scores improved at final follow-up for knees with K-L grade ≥ 2 osteoarthritis (p < 0.001), with no significant difference compared to K-L 0/1. Patients with Outerbridge class 3/4 cartilage degeneration also reported improvements in clinical scores, albeit lower than those with class 2 degeneration (p < 0.05). During recovery, the majority of patients reported clinical improvements by 6 months, and six patients further improved by at least 15 points in IKDC score between 6 and 12 months. Osteoarthritis progressed in 10 of 31 knees (32%), with an overall mean pre-operative K-L grade of 1.6 ± 0.9 compared to 2.0 ± 0.9 at final follow-up (n.s.). No knees progressed to K-L 4 or underwent re-operation. Pre-operative bone marrow oedema was present in 17 knees (52%), all of which had signal localised to the medial tibia or femur. Oedema had resolved in all but 5 knees post-operatively (p < 0.01).

Conclusion
Arthroscopic repair of medial meniscus posterior root tears is associated with improved outcomes in knees with advanced cartilage degeneration and osteoarthritis. Meaningful improvements in clinical outcomes can be achieved beyond 6 months, thus success of the operation is best determined at the 12-month mark. Oedema signal significantly improved post-operatively, however a relatively high proportion of knees had K-L progression.

Level of evidence
IV – Case Series.
Peri-implant bone-level changes in the second decade of loading with regard to the implant–abutment connection: a retrospective study on implants under systematic aftercare
Authors: Kruse Anne Brigitte , Wild Vanessa , Ratka-Krüger Petra , Vach Kirstin , Frisch Eberhard 
Published: September 2021

Abstract
Background
This retrospective study investigates the change in the peri-implant bone level (PBL) during the 2nd decade of intraoral function in patients complying with a ‘supportive implant therapy’ (SIT) program. The results were statistically analyzed with respect to the implant abutment connection used.

Methods
In a private practice, only patients with 20-year SIT compliance were identified. Of these, all patients with 10- and 20-year radiographs available were selected. Therefore, no control group was possible and implant losses had to be excluded. Two experienced researchers assessed the peri-implant bone levels. As three different abutment connection concepts (bone-level butt-joint, bone-level conical and tissue-level conical) and two different implant surfaces (machined vs. roughened) were involved, statistical analyses were performed to detect potential differences.

Results
Ninety-three implants from 36 patients with 20-year SIT compliance and available radiographs were included in the study. At study baseline (10 years intraoral), a mean bone loss of − 1.7 mm (median − 1.2; standard deviation [sd] 1.4, range: 0 to − 7.2) was recorded. After 20 years, we found a mean bone loss of − 2.5 mm (median − 2.3, sd 1.79, range: − 0.5 to + 7.4). Furthermore, we found a mean bone loss of 0.8 mm in intraoral function from year 10 to year 20 (mean: 0.08 mm per year); this change was independent of the abutment connection type.

Conclusions
During the 2nd decade of function, peri-implant bone loss in patients with SIT compliance might be small in value and should not be expected in all implants.
Salvage endovascular embolization of the left pulmonary artery for recurrent pseudoaneurysm
Authors: Yutaka Yojiro , Tasaki Junichi , Yuasa Itsuki , Murakami Kotaro , Date Hiroshi 
Published: September 2021

Abstract
Background
Pulmonary pseudoaneurysm (PPA) is a potentially lethal complication of lung resection with a high risk of recurrence after endovascular coiling.

Case presentation
We report a case in which recurrent hemoptysis due to PPA after left lower lobe sleeve resection was treated by endovascular embolization of the left main pulmonary artery as a salvage treatment. The first hemoptysis was managed by endovascular coil embolization with extracorporeal membrane oxygenation, but refractory hemorrhage occurred 3 months later due to penetration of the endovascular coil into the bronchial anastomosis site. Because left completion pneumonectomy was considered too high risk, the left main pulmonary artery was palliatively embolized using an Amplatzer vascular plug (St. Jude Medical, MN, USA) to totally disrupt the left pulmonary arterial flow.

Conclusions
Total embolization of the left main pulmonary artery for repeated PPA rupture may be useful as a palliative treatment in patients unable to tolerate pneumonectomy.
Rationale and design of the PRevention of cArdiac Dysfunction during Adjuvant breast cancer therapy (PRADA II) trial: a randomized, placebo-controlled, multicenter trial
Authors: Mecinaj A , Gulati G , Heck SL , Holte E , Fagerland MW , Larsen AI , Blix E.S , Geisler J , Wethal T , Omland T 
Published: September 2021

Abstract
Background
Recent advances in the treatment algorithms of early breast cancer have markedly improved overall survival. However, anthracycline- and trastuzumab-associated cardiotoxicity may lead to dose-reduction or halt in potentially life-saving adjuvant cancer therapy. Early initiated neurohormonal blockade may prevent or attenuate the cardiotoxicity-induced reduction in cardiac function, but prior studies have been inconclusive. The angiotensin receptor-neprilysin inhibitor sacubitril/valsartan has been shown to be superior to traditional treatment in heart failure with reduced ejection fraction, but its cardioprotective effects in the cardio-oncology setting remains to be tested.

Objective
To assess if sacubitril/valsartan given concomitantly with early breast cancer treatment regimens including anthracyclines, with or without trastuzumab, may prevent cardiac dysfunction.

Methods
PRADA II is a randomized, placebo-controlled, double blind, multi-center, investigator-initiated clinical trial. Breast cancer patients from four university hospitals in Norway, scheduled to receive (neo-)adjuvant chemotherapy with epirubicin independently of additional trastuzumab/pertuzumab treatment, will be randomized 1:1 to sacubitril/valsartan or placebo. The target dose is 97/103 mg b.i.d. The patients will be examined with cardiovascular magnetic resonance (CMR), echocardiography, circulating cardiovascular biomarkers and functional testing at baseline, at end of anthracycline treatment and following 18 months after enrolment. The primary outcome measure of the PRADA II trial is the change in left ventricular ejection fraction (LVEF) by CMR from baseline to 18 months. Secondary outcomes include change in LV function by global longitudinal strain by CMR and echocardiography and change in circulating cardiac troponin concentrations.

Results
The study is ongoing. Results will be published when the study is completed.

Conclusion
PRADA II is the first randomized, placebo-controlled study of sacubitril/valsartan in a cardioprotective setting during (neo-)adjuvant breast cancer therapy. It may provide new insight in prevention of cardiotoxicity in patients receiving adjuvant or neo-adjuvant therapy containing anthracyclines. Furthermore, it may enable identification of patients at higher risk of developing cardiotoxicity and identification of those most likely to respond to cardioprotective therapy.

Trial registration
The trial is registered in the ClinicalTrials.gov registry (identifier NCT03760588). Registered 30 November 2018.
Synthesized HMGB1 peptide attenuates liver inflammation and suppresses fibrosis in mice
Authors: Nojiri Shunsuke , Tsuchiya Atsunori , Natsui Kazuki , Takeuchi Suguru , Watanabe Takayuki , Kojima Yuichi , Watanabe Yusuke , Kamimura Hiroteru , Ogawa Masahiro , Motegi Satoko , Iwasawa Takahiro , Sato Takeki , Kumagai Masaru , Ishii Yui , Kitayama Tomomi , Li Yu-Tung , Ouchi Yuya , Shimbo Takashi , Takamura Masaaki , Tamai Katsuto , Terai Shuji 
Published: September 2021

AbstractThe liver has a high regenerative ability and can induce spontaneous regression of fibrosis when early liver damage occurs; however, these abilities are lost when chronic liver damage results in decompensated cirrhosis. Cell therapies, such as mesenchymal stem cell (MSC) and macrophage therapies, have attracted attention as potential strategies for mitigating liver fibrosis. Here, we evaluated the therapeutic effects of HMGB1 peptide synthesized from box A of high mobility group box 1 protein. Liver damage and fibrosis were evaluated using a carbon tetrachloride (CCl4)-induced cirrhosis mouse model. The effects of HMGB1 peptide against immune cells were evaluated by single-cell RNA-seq using liver tissues, and those against monocytes/macrophages were further evaluated by in vitro analyses. Administration of HMGB1 peptide did not elicit a rapid response within 36 h, but attenuated liver damage after 1 week and suppressed fibrosis after 2 weeks. Fibrosis regression developed over time, despite continuous liver damage, suggesting that administration of this peptide could induce fibrolysis. In vitro analyses could not confirm a direct effect of HMGB1 peptide against monocyte/macrophages. However, macrophages were the most affected immune cells in the liver, and the number of scar-associated macrophages (Trem2+Cd9+ cells) with anti-inflammatory markers increased in the liver following HMGB1 treatment, suggesting that indirect effects of monocytes/macrophages were important for therapeutic efficacy. Overall, we established a new concept for cell-free therapy using HMGB1 peptide for cirrhosis through the induction of anti-inflammatory macrophages.
Prevalence, antimicrobial susceptibility and genotypic characteristics of Staphylococcus aureus in Tanzania: a systematic review
Authors: Mzee Tutu , Kazimoto Theckla , Madata Joseph , Masalu Rose , Bischoff Markus , Matee Mecky , Becker Sören L. 
Published: September 2021

AbstractBackgroundData on the prevalence, genotypes and antibiotic resistance patterns of colonizing and infection-associatedStaphylococcus aureus(S. aureus) strains both in humans and animals in Tanzania are scarce. Given the wide range of infections caused byS. aureusand the rise of methicillin-resistantS. aureus(MRSA) globally, this review aims at collecting published data onS. aureusbacterium to improve our understanding of its epidemiology in Tanzania.Main bodyWe carried out a systematic review of scientific studies reporting on prevalence, antibiotic resistance and genotyping data pertaining toS. aureusin human and animal infection and colonization. The literature extracted from electronic databases such as PubMed and Google Scholar was screened for eligibility and relevant articles were included. The review is limited to manuscripts published in English language between the years 2010 and 2020. A total of 45 studies conducted in 7 of the 9 administrative zones in Tanzania were reviewed to gather data onS. aureusprevalence in humans and animals. Prevalence in humans ranged from 1 to 60%. Antibiotic resistance patterns ofS. aureusisolated from colonized humans showed high resistance rates against co-trimoxazole (46%) and erythromycin (41%) as compared to reports from studies conducted outside Africa. The review suggests an increased MRSA prevalence of up to 26% as compared to 6–16% reported in previous years. Genotypic data reviewed suggested that MRSA predominantly belonged to ST88. The prevalence ofS. aureusin animal studies ranged from 33 to 49%, with 4 to 35% of MRSA isolates. Most studies reported low antibiotic resistance levels, with the exception of penicillin (85%) and ampicillin (73%).ConclusionThe prevalence ofS. aureusand MRSA in Tanzania is rising, although clear variations between different geographic areas could be observed. Non-susceptibility to commonly prescribed antibiotics in community-associatedS. aureusis of concern. Research strategies to ameliorate our knowledge onS. aureusepidemiology should employ regular antibiotic resistance surveillance, antimicrobial stewardship as well as genotypic characterization.
Currarino syndrome or Hirschsprung disease: how to prevent diagnostic dilemma in chronic constipation
Authors: Ali Md Mokarram , Singh Ram Jeewan , Rashi Rashi , Kumar Amit , Sinha Amit Kumar , Kumar Bindey 
Published: September 2021

Abstract
Background
Currarino syndrome is a rare congenital malformation having autosomal dominant inheritance. It comprises of anorectal malformation, presacral mass, and sacral vertebral defect occurring in variable proportion. The most common presentation is chronic constipation which is usually due to compression of rectum by anterior sacral mass. If clinical examination is not properly done and digital rectal examination is excluded from the examination, it can be misdiagnosed as other common cause of constipation like Hirschsprung disease.

Case presentation
We are reporting one such case of one-and-half-year-old female child with chronic constipation which was initially managed as Hirschsprung disease, but later on, after a repeat clinical examination with digital rectal examination, it was evaluated on the line of Currarino syndrome. The diagnosis was confirmed by contrast-enhanced computed tomography of abdomen with 3 dimensional reconstruction. It was then managed by posterior sagittal approach with excision of mass and anorectoplasty.

Conclusion
A proper protocol for clinical evaluation of patient with constipation prevents diagnostic dilemma between surgical causes of constipation in pediatric age group. Digital rectal examination must be included in the protocol for evaluation of chronic constipation. In pediatric age group, clinical workup should be done with keeping in mind the rare diagnosis of Currarino syndrome along with common cause of constipation like Hirschsprung disease.
Hearing Loss After Radiation and Chemotherapy for CNS and Head-and-Neck Tumors in Children
Authors: Keilty Dana , Khandwala Mohammad , Liu Zhihui Amy , Papaioannou Vicky , Bouffet Eric , Hodgson David , Yee Ryan , Cushing Sharon , Laperriere Normand , Ahmed Sameera , Mabbott Donald , Ramaswamy Vijay , Tabori Uri , Huang Annie , Bartels Ute , Tsang Derek S. 
Published: September 2021

PURPOSE Hearing loss (HL) is a serious secondary effect of treatment for CNS and head-and-neck tumors in children. The goal of this study was to evaluate incidence and risk factors for HL in patients with multiple ototoxic exposures. PATIENTS AND METHODS We evaluated 340 ears from 171 patients with CNS or head-and-neck tumors treated with radiation, with or without chemotherapy, who had longitudinal audiologic evaluation. International Society of Pediatric Oncology-Boston grades were assigned to 2,420 hearing assessments. Multivariable weighted ordinal logistic regression was fitted to evaluate the effect of clinicopathologic features on HL. RESULTS Mean cochlea dose (odds ratio [OR] 1.04 per Gy, P < .001), time since radiotherapy (RT; OR 1.21 per year, P < .001), cisplatin dose (OR 1.48 per 100 mg/m2, P < .001), and carboplatin dose (OR 1.41 per 1,000 mg/m2, P = .002) were associated with increasing International Society of Pediatric Oncology-Boston grade of HL. There was no synergistic effect of RT and cisplatin (interaction term, P = .53) or RT and carboplatin (interaction term, P = .85). Cumulative incidence of high-frequency HL (> 4 kHz) was 50% or greater at 5 years after RT if mean cochlea dose was > 30 Gy, while incidence of HL across all frequencies continued to increase beyond 5 years after RT. CONCLUSION Children treated with radiation and chemotherapy experience a high incidence of HL over time, with associations found between more severe HL and cisplatin or carboplatin dose as well as mean cochlea dose. Mean cochlea dose of ≤ 30 Gy is proposed as a goal to reduce the risk of HL; a lower threshold (20-25 Gy) may be considered in patients receiving platinum chemotherapy to reduce cumulative HL burden.
College as a Great Equalizer? Marriage and Assortative Mating Among First- and Continuing-Generation College Students
Authors: King Michael D. 
Published: September 2021

AbstractCollege has been hailed as a “great equalizer” that can substantially reduce the influence of parents' socioeconomic status on their children's subsequent life chances. Do the equalizing effects of college extend beyond the well-studied economic outcomes to other dimensions, in particular, marriage? When and whom one marries have important implications for economic and family stability, with marriage acting as a social safety net, encouraging joint long-term investments, and potentially producing dual-earner families. I focus on the marriage timing and assortative mating patterns of first- and continuing-generation college graduates to test whether college acts as an equalizer for marriage against alternative hypotheses. Using discrete-time event-history methods and data from the National Longitudinal Survey of Youth 1997, I find small differences between first- and continuing-generation graduates in marriage timing, but larger differences in assortative mating, particularly for women. First-generation women have a substantially lower likelihood of marrying another college graduate than do continuing-generation women, and a higher likelihood of marrying a noncollege graduate. These findings highlight the importance of examining noneconomic outcomes when studying social mobility and offer insight into how inequality may persist across generations, especially for women, despite apparent upward mobility.
Uncertainty About the Size of the Unauthorized Foreign-Born Population in the United States
Authors: Van Hook Jennifer , Morse Anne , Capps Randy , Gelatt Julia 
Published: September 2021

Abstract
One of the most common methods for estimating the U.S. unauthorized foreign-born population is the residual method. Over the last decade, residual estimates have typically fallen within a narrow range of 10.5 to 12 million. Yet it remains unclear how sensitive residual estimates are to their underlying assumptions. We examine the extent to which estimates may plausibly vary owing to uncertainties in their underlying assumptions about coverage error, emigration, and mortality. Findings show that most of the range in residual estimates derives from uncertainty about emigration rates among legal permanent residents, naturalized citizens, and humanitarian entrants (LNH); estimates are less sensitive to assumptions about mortality among the LNH foreign-born and coverage error for the unauthorized and LNH populations in U.S. Census Bureau surveys. Nevertheless, uncertainty in all three assumptions contributes to a range of estimates, whereby there is a 50% chance that the unauthorized foreign-born population falls between 9.1 and 12.2 million and a 95% chance that it falls between 7.0 and 15.7 million.