bluuebunny/crossref_metadata_2025_split huggingface dataset:
Leaked genomic and mitochondrial DNA contribute to the host response to noroviruses in a STING-dependent manner
Authors: Jahun Aminu S. , Sorgeloos Frederic , Chaudhry Yasmin , Arthur Sabastine E. , Hosmillo Myra , Georgana Iliana , Izuagbe Rhys , Goodfellow Ian G. 
Published: August 2021

AbstractThe cGAS-STING pathway is central to the IFN response against DNA viruses. However, recent studies are increasingly demonstrating its role in the restriction of some RNA viruses. Here we show that the cGAS-STING pathway also contributes to the IFN response against noroviruses, positive-sense single-stranded RNA viruses that are now one of the most common causes of infectious gastroenteritis world-wide. We show a significant reduction in IFN-β induction and a corresponding increase in viral replication in norovirus-infected cells following STING inhibition, knockdown or deletion. Upstream of STING, we show that cells lacking either cGAS or IFI16 also have severely impaired IFN responses. Further, we demonstrate that immunostimulatory host genome-derived DNA, and to a lesser extent mitochondrial DNA, accumulate in the cytosol of norovirus-infected cells. And lastly, overexpression of the viral NS4 protein was sufficient to drive the accumulation of cytosolic DNA. Together, our data elucidate a role for cGAS, IFI16 and STING in the restriction of noroviruses, and demonstrate for the first time the utility of host genomic DNA as a damage-associated molecular pattern in cells infected with an RNA virus.HighlightscGAS, IFI16 and STING are required for a robust IFN response against norovirusesNuclear and mitochondrial DNA accumulate in the cytosols of infected cellsViral NS4 mediates accumulation of cytosolic DNA
Variation in synonymous nucleotide composition among genomes of sarbecoviruses and consequences for the origin of COVID-19
Authors: Hassanin Alexandre 
Published: August 2021

AbstractThe subgenus Sarbecovirus includes two human viruses, SARS-CoV and SARS-CoV-2, respectively responsible for the SARS epidemic and COVID-19 pandemic, as well as many bat viruses and two pangolin viruses.Here, the synonymous nucleotide composition (SNC) of Sarbecovirus genomes was analysed by examining third codon-positions, dinucleotides, and degenerate codons. The results show evidence for the eigth following groups: (i) SARS-CoV related coronaviruses (SCoVrC including many bat viruses from China), (ii) SARS-CoV-2 related coronaviruses (SCoV2rC; including five bat viruses from Cambodia, Thailand and Yunnan), (iii) pangolin viruses, (iv) three bat viruses showing evidence of recombination between SCoVrC and SCoV2rC genomes, (v) two highly divergent bat viruses from Yunnan, (vi) the bat virus from Japan, (vii) the bat virus from Bulgaria, and (viii) the bat virus from Kenya. All these groups can be diagnosed by specific nucleotide compositional features except the one concerned by recombination between SCoVrC and SCoV2rC. In particular, SCoV2rC genomes are characterised by the lowest percentages of cyosine and highest percentages of uracil at third codon-positions, whereas the genomes of pangolin viruses exhibit the highest percentages of adenine at third codon-positions. I suggest that latitudinal and taxonomic differences in the imbalanced nucleotide pools available in host cells during viral replication can explain the seven groups of SNC here detected among Sarbecovirus genomes. A related effect due to hibernating bats is also considered. I conclude that the two independent host switches from Rhinolophus bats to pangolins resulted in convergent mutational constraints and that SARS-CoV-2 emerged directly from a horseshoe bat virus.
An Algorithm to Quantify Inducible Protein Condensates In Eukaryotic Cells
Authors: Hunn Jeremy C. , Hutchinson Katherine M. , Kelley Joshua B. , Reines Daniel 
Published: August 2021

ABSTRACTReorganization of cellular proteins into subcellular compartments, such as the rearrangement of RNA-binding proteins into cytoplasmic stress granules and P-bodies, is a well-recognized, widely studied physiological process currently under intense investigation. Using the assembly of a novel, inducible, nuclear granule formed from the yeast RNA-binding transcription termination factors Nab3 and Nrd1, we present a freely-accessible, high-throughput and unbiased algorithm written in MATLAB that detects and measures protein distribution, partitioning, and sequestration into subcellular compartments captured by fluorescence microscopy; an invaluable advancement to current image analysis methods which utilize experiment-specific custom scripts or subjective manual counting. Employing our algorithm, we quantified thousands of cells, ensuring rigorous examination of Nab3 granule formation across strains with reproducible statistical analyses. We document strain differences in Nab3 granule formation and an associated growth defect. Additionally, we applied our algorithm to immunofluorescent images of the inducible polymerization into filaments of an enzyme in human cells, demonstrating the algorithm’s versatility and adaptability.SUMMARY STATEMENTWe describe a computational tool that enables the quantification of protein condensation during assembly of a subnuclear compartment. The algorithm scores assembly of fluorescently tagged proteins in yeast or human cells.
Identification of novel BDNF-specific corticostriatal circuitries
Authors: Ehinger Yann , Soneja Drishti , Phamluong Khanhky , Salvi Alexandra , Ron Dorit 
Published: August 2021

AbstractBDNF is released from axon terminals originating in the cerebral cortex onto striatal neurons. Here, we characterized BDNF neurons in the corticostriatal circuitry. First, we utilizedBDNF-Cre and Ribotag transgenic mouse lines to label BDNF-positive neurons in the cortex and detectedBDNFexpression in all the subregions of the prefrontal cortex (PFC). Next, we used a retrograde viral tracing strategy, in combination withBDNF-Cre knockin mice, to map the cortical outputs of BDNF neurons in the dorsomedial and dorsolateral striatum (DMS and DLS). We found thatBDNF-expressing neurons located in the medial prefrontal cortex (mPFC) project mainly to the DMS, and those located in the primary and secondary motor cortices (M1 and M2) and agranular insular cortex (AI) project mainly to the DLS. In contrast,BDNF-expressing orbitofrontal cortical (OFC) neurons differentially target the dorsal striatum (DS) depending on their mediolateral and rostrocaudal location. Specifically, the DMS is mainly innervated by the medial and ventral part of the orbitofrontal cortex (MO and VO) whereas the DLS receives projections specifically from the lateral part of the OFC (LO). Together, our study uncovers previously unknown BDNF corticostriatal circuitries. These findings could have important implications for the role of BDNF signaling in corticostriatal pathways.Significance StatementBDNF is released in axons upon neuronal depolarization. Surprisingly, careful mapping of BDNF projecting neurons in the central nervous system (CNS) has not been conducted. Using retrograde viral strategies in combination with transgenic mice, we mapped out corticostriatal BDNF circuits. We found that, mPFC BDNF neurons project mainly to the DMS whereas the motor cortex and AI project to the DLS. BDNF neurons in the OFC are anatomically segregated. Whereas the DMS receives BDNF-positive projections from the VO, the DLS mainly receives BDNF-positive projections from the LO. Our findings could be important to the study of BDNF in corticostriatal circuitries.
From Alpha to Zeta: Identifying variants and subtypes of SARS-CoV-2 via clustering
Authors: Melnyk Andrew , Mohebbi Fatemeh , Knyazev Sergey , Sahoo Bikram , Hosseini Roya , Skums Pavel , Zelikovsky Alex , Patterson Murray 
Published: August 2021

AbstractThe availability of millions of SARS-CoV-2 sequences in public databases such as GISAID and EMBL-EBI (UK) allows a detailed study of the evolution, genomic diversity and dynamics of a virus like never before. Here we identify novel variants and sub-types of SARS-CoV-2 by clustering sequences in adapting methods originally designed for haplotyping intra-host viral populations. We asses our results using clustering entropy — the first time it has been used in this context.Our clustering approach reaches lower entropies compared to other methods, and we are able to boost this even further through gap filling and Monte Carlo based entropy minimization. Moreover, our method clearly identifies the well-known Alpha variant in the UK and GISAID datasets, but is also able to detect the much less represented (< 1% of the sequences) Beta (South Africa), Epsilon (California), Gamma and Zeta (Brazil) variants in the GISAID dataset. Finally, we show that each variant identified has high selective fitness, based on the growth rate of its cluster over time. This demonstrates that our clustering approach is a viable alternative for detecting even rare subtypes in very large datasets.
Adaptation in outbred sexual yeast is repeatable, polygenic, and favors rare haplotypes
Authors: Linder Robert A. , Zabanavar Behzad , Majumder Arundhati , Hoang Hannah Chiao-Shyan , Delgado Vanessa Genesaret , Tran Ryan , La Vy Thoai , Leemans Simon William , Long Anthony D 
Published: August 2021

AbstractWe describe the results of a 200 generation Evolve and Resequence (E&R) study initiated from an outbred dipliod recombined synthetic base population derived from 18 genetically diverse founders. Replicate populations were maintained at large effective population sizes (>105 individuals), exposed to several different chemical challenges over 12 weeks of evolution, and whole-genome resequenced. Weekly forced outcrossing implies a per gene per cell-division recombination rate higher than that achieved in Drosophila E&R studies. In 55 sexual populations we observe large fitness gains and highly repeatable patterns of genome-wide haplotype change within each chemical challenge. There was little evidence for pervasive pleiotropy, as evidenced by patterns of haplotype change between drug treatments. Within treatment adaptation appears highly polygenic with almost the entire genome showing significant consistent haplotype change. Finally, adaptation was almost always associated with only one of the 18 founder alleles, suggesting selection primarily acts on rare variants private to a founder or haplotype blocks harboring multiple mutations. This observation contradicts the notion that adaptation is often due to subtle frequency shifts at intermediate frequency variants.
The Eastern construction of paradoxical cognitive framework and its antecedents: a Yin–Yang balancing perspective
Authors: Yang Yong , Fan Youqing , Jia Jianfeng 
Published: August 2021

Purpose
In the face of external paradoxical requirements, the cognitive framework of managers and employees use to perceive, interpret and reconstruct information is important to ease anxiety and improve job performance. The Yin-Yang balancing of eastern philosophical thought is particularly good at explaining and predicting changes and conflict environments. For this reason, this study aims to propose the eastern construction of the paradoxical cognitive framework based on the Yin-Yang balancing theory and its antecedent framework.

Design/methodology/approach
This paper contrasts the similarity and differences between Chinese and Western philosophy’s thoughts on paradoxes. On this basis, the eastern construction of the paradoxical cognitive framework is proposed. Then, the paper puts forward the antecedent framework of managers’ cognitive framework and employees’ paradoxical cognitive framework.

Findings
This paper proposes the eastern construction of the paradoxical cognitive framework includes the following three dimensions: the unity-in-diversity of paradoxical elements, the asymmetric balance of paradoxical elements and mutual transformation of paradoxical elements. In addition, this paper proposes an antecedent framework of the eastern construction of the paradoxical cognitive framework – the paradoxical requirement of organizational environment exerts a direct impact on managers’ and employees’ paradoxical cognitive framework; managers’ paradoxical cognitive framework has a positive impact on paradoxical leadership; paradoxical leadership exerts an indirect impact on employees’ paradoxical cognitive framework through the collective paradoxical cognitive framework; paradoxical leadership directly affects employees’ paradoxical cognitive framework.

Research limitations/implications
This paper focuses on comparing the similarities and differences of the individual paradoxical cognitive framework in Chinese and Western cultures and proposes the eastern construction of the paradoxical cognitive framework and its antecedent framework. Future research needs to further verify the theoretical framework proposed in this paper.

Originality/value
This paper makes a detailed comparison of the paradox views in Chinese and Western philosophy. It is the first to propose the eastern construction of the paradoxical cognitive framework and its antecedent framework, laying a theoretical foundation for future empirical research.
Alone but together: flow experience and its impact on creative output in LEGO® SERIOUS PLAY®
Authors: Zenk Lukas , Primus Dirk J. , Sonnenburg Stephan 
Published: August 2021

PurposeDo LEGO® SERIOUS PLAY® (LSP) workshops result in improved experience of flow components as well as higher levels of creative output than traditional meetings (MEET)? This research studies the extent to which LSP, as a specialized material-mediated and process-oriented cocreative workshop setting, differs from MEET, a traditional workshop setting. Hypotheses for differences in individual flow components (autotelic behavior, happiness, balance), group flow components (equal participation, continuous communication) and creative output were developed and tested in a quasi-experimental comparison between LSP and MEET.Design/methodology/approachThe study was conducted with 39 practitioners in six teams from various industries. In total, 164 observations were collected during two workshops using the Experience Sampling Method. The creative output was assessed by peer evaluations of all participants, followed by structural analysis and quantitative group comparisons.FindingsThe results show that two components of individual flow experience (autotelic behavior, happiness) were significantly higher in LSP, and one of the components of group flow experience (continuous communication) was, as expected, significantly lower. Regarding creative output, the LSP teams outperformed the MEET teams. The study suggests that a process-oriented setting that includes time for individuals to independently explore their ideas using a different kind of material in the presence of other participants has a significant influence on the team result.Practical implicationsLSP can improve the components of participants' flow experience to have an impact on the creative output of teams. In cocreative settings like LSP, teams benefit from a combination of alone time and high-quality collaborative activities using boundary objects and a clear process to share their ideas.Originality/valueThis is the first quasi-experimental study with management practitioners as participants to compare LSP with a traditional and widespread workshop approach in the context of flow experience and creative output.
Role of artificial intelligence and robotics to foster the touchless travel during a pandemic: a review and research agenda
Authors: Gaur Loveleen , Afaq Anam , Singh Gurmeet , Dwivedi Yogesh Kumar 
Published: August 2021

PurposeThe hospitality industry experienced an unanticipated challenge from the COVID-19 pandemic. However, research in this area is scarce. Accordingly, this study aims to unfold a three-angled research agenda to intensify the knowledge advancement in the hospitality sector. It proposes a theoretical framework by extending the protection motivation theory (PMT) to explain the guest’s intent to adopt artificial intelligence (AI) and robotics as a protective measure in reaction to COVID-19.Design/methodology/approachThe research is centered on outlining the pertinent literature on hospitality management practices and the guest’s transformed behavior during the current crisis. This study intends to identify a research agenda based on investigating hospitality service trends in today’s changing times.FindingsThe study sets out a research agenda that includes three dimensions as follows: AI and robotics, cleanliness and sanitation and health care and wellness. This study’s findings suggest that AI and robotics may bring out definite research directions at the connection of health crisis and hospitality management, taking into account the COVID-19 crisis.Practical implicationsThe suggested research areas are anticipated to propel the knowledge base and help the hospitality industry retrieve the COVID-19 crisis through digital transformation. AI and robotics are at the cusp of invaluable advancement that can revive the hotels while re-establish guests’ confidence in safe hotel practices. The proposed research areas are likely to impart pragmatic lessons to the hospitality industry to fight against disruptive situations.Originality/valueThis study stands out to be pioneer research that incorporated AI and robotics to expand the PMT and highlights how behavioral choices during emergencies can bring technological revolution.
The direct and indirect effects of parental trauma on child adjustment for resettled refugees in Australia
Authors: Kara Behice Humeyra , Stuart Jaimee 
Published: August 2021

Purpose
Understanding the effects trauma has on refugee parents and consequently, their children, is the first step in interrupting the intergenerational transmission of trauma. This study aims to investigate the impacts of parental exposure to trauma pre-settlement on parent and child reports of developmental difficulties as mediated by parental post-traumatic stress symptomology and harsh parenting.

Design/methodology/approach
The study included 414 refugee children (age M = 14.04, SD = 2.00; 48.3% female) and their caregivers (age M = 41.78, SD = 5.24, 77% female). The sample was drawn from the Building a New Life in Australia study, a large, representative cohort study of resettled refugees in Australia. Only data collected where both parents and their children could be matched were used in this study.

Findings
Results indicated that trauma was significantly associated with increased parental post-traumatic stress disorder (PTSD) symptoms in all models and was negatively, albeit weakly, associated with lower levels of harsh parenting in the overall model which combined parent and child reports. Trauma also had a weak, positive indirect effect on developmental difficulties via parental PTSD in both the overall model and the model assessing parent-rated developmental difficulties. In all models, harsh parenting was associated with increased developmental difficulties, although harsh parenting did not act as a significant mediator of the effects of trauma or parental PTSD.

Originality/value
Results suggest that prior traumas had less of an adverse effect on parenting and child adjustment as was expected. Parenting, however, was strongly associated with poor child adjustment, indicating that this may be a key factor to encourage positive adjustment for refugee children.
Impact of mass collaboration on knowledge sharing process using mediating role of innovation capability
Authors: Sultan Abdulsatar Abduljabbar , Alfaiza Salsabila Aisyah , Riyadh Hosam Alden 
Published: August 2021

Purpose
The purpose of this paper is to determine the impact of mass collaboration on the knowledge-sharing process in the manufacturing sector of Iraq in the mediation of innovation capability.

Design/methodology/approach
This study is quantitative in nature in which the data has been gathered from the primary sources of information through a survey questionnaire from 225 respondents. The structural equation structural equation modeling technique has been used to analyze the data along with the path assessment and confirmatory factor analysis confirmatory factor analysis.

Findings
The results of this study determined that in the context of the interactive system and mutual adjustment, the mediation of innovation capability is partial whereas, in the context of shared understating, the mediation is full. In addition to this, an interactive system and mutual adjustment had a significant effect on the knowledge sharing process, while the shared understanding was determined to have an insignificant effect on the knowledge sharing process.

Research limitations/implications
The findings of this study are limited to the manufacturing sector of Iraq. In addition to this, the study includes a limited sample size which must be increased by future researchers.

Originality/value
This paper ensures originality in the context of Iraq, as the associations tested in this study have not been tested previously considering Iraq.
Investigating service innovation, bank reputation and customer trust: evidence from Indian retail banking
Authors: Biswas Abhijeet , Jaiswal Deepak , Kant Rishi 
Published: August 2021

Purpose
Global proliferation had a noteworthy impact on the Indian retail banking industry and mushrooming banks have entailed to emphasize on customer satisfaction (CS) quotient to remain competitive. This study aims to illuminate determinants of CS and customer trust (CT) and their denouement in Indian retail banking.

Design/methodology/approach
The top six Indian private sector banks were selected hinged on market capitalization. A total of 460 responses from retail bank customers were gathered using a structured questionnaire. Direct and indirect relationships were analyzed with mediation and moderation by using structural equation modeling.

Findings
The study identifies determinants of CS and CT. The results manifest that perceived service innovation (PSI) and bank reputation (BR) considerably aggrandize CS and CT. Furthermore, CS strikingly magnifies customer loyalty (CL). The study also posits that CT partially mediates between the nexus of PSI and BR on the one side and CS on the other. Moreover, perceived risk moderates the association between CS and loyalty.

Research limitations/implications
The study demonstrates indispensable drivers of CS, CT and CL which may encourage bank professionals to hold on to their customers and enhance profitability.

Originality/value
Here is a dearth of literature on PSI and BR in the Indian banking industry. Thus, the study supplements literature by assimilating these constructs through a compendious conceptual model. The study is distinct as it evaluates mediating effects of CT in unfurling complexities of relationships among the underlying constructs.