bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification of Genes and Pathways of Nonsteroidal Anti-inflammatory Drugs acting on Synovia from Women with Knee Osteoarthritis by Bioinformatics Analysis
Authors: Zhao Chao , Wang Han , Dong Conglei , Kang Huijun , Wang Fei 
Published: April 2021

Abstract
Objective: Through the bioinformatics analysis, to identify the genes and pathways of nonsteroidal anti-inflammatory drugs(NSARDs) acting on synovia from women with knee osteoarthritis (KOA), and to provide reference for clinical application. Methods: We downloaded the gene microarray datasets with the accession number of GSE55457 and GSE55584 from the Gene Expression Omnibus (GEO; https://www.ncbi.nlm.nih.gov/geo/) database, including 5 untreated KOA patients, 9 NSARDs treated KOA patients and 2 patients without KOA The samples in the untreated KOA group and the NSARDs treated KOA group were used for main analysis. The samples in the untreated KOA group and the normal control group were used for cooperative analysis. Then we performed robust multi-array (RMA) normalization with affy R programming package. After that, differential expression genes (DEGs) in main analysis and cooperative analysis were identified based on limma package separately. Screening the common DEGs from main analysis and cooperative analysis. Enriched gene ontology (GO) terms and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways of DEGs were obtained through the Database for Annotation, Visualization and Integrated Discovery (DAVID). What's more, protein-protein interaction (PPI) network was constructed, and we identified modules of PPI network through Cytoscape to screen valuable targets. The value of gene expression fold change (FC) ≥1.4 or ≤1/1.4, and P <0.05 were used as the screening conditions. P <0.05 and Associated genes count>5 were used as the screening conditions.Results: There were 338 DEGs in main analysis. Among them, 211 genes were up-regulated and 127 genes were down-regulated. There were 7005 DEGs in cooperative analysis. Among them, 6952 genes were up-regulated and 53 genes were down-regulated. A total of 129 common DEGs were identified between main analysis and cooperative analysis. There are 2 biological processes, 3 cell components and 2 molecular functions for the enrichment of differentially expressed genes.Conclusion: NSARDs may play a certain role in synovia from women with KOA by regulating the mRNA expressions of il-6, TNFRSF11A and CSF1R, which may become one of the indicators for monitoring the efficacy of NSAIDs.
Dissociative Symptoms and Psychotic Features in Bipolar Disorder: Impact on Clinical Course and Treatment Response
Authors: Steardo Luca , Carbone Elvira Anna , Ventura Enrica , Filippis Renato de , Luciano Mario , Fazio Pasquale De 
Published: April 2021

Abstract
Background

Dissociative symptoms are widely expressed in patients with bipolar disorder (BD) and may precede the onset of the disorder and be a marker of poor treatment response. In the present study we aimed: 1) to assess the relationship between dissociative symptoms and the onset of psychotic symptoms in patients with BD; 2) to assess clinical and socio-demographic characteristics more frequently associated with dissociative symptoms and the response to treatment with mood stabilizers.
Methods

One-hundred patients diagnosed with BD were enrolled in this study. They underwent a semi-structured interview to collect socio-demographic and clinical characteristics. Dissociative Experiences Scale-II (DES-II) and ALDA scale were used to assess dissociative psychopathologies and response to treatment with mood stabilizers respectively.
Results

Forty-four percent of patients reported psychotic symptoms on the DES-II scale; BD I patients had a higher total DES-II score than BD II patients. Dissociative symptoms presented a direct correlation with the total number of episodes (p < .000), antidepressant switch to mania (p < .000), seasonality (p < .000), aggression (p < .000), and mixed states (p < .000). higher DES-II scores were reported by patients with poor response to treatment.
Conclusions

Dissociative phenomena are closely related to the presence of psychotic symptoms in BD, especially in BD I. In a future perspective, this association could represent a diagnostic indicator of BD I have given the close association with psychotic symptoms. Our study shows that several clinical variables transversely indicate a poor response to treatment with mood stabilizers, a worse course of illness, and the presence of dissociative symptoms.
Preliminary Study of Coupling Intervals of Premature Ventricular Complexes in Dogs With Different Cardiac Diseases
Authors: Koster Liza Sally , Abbott Jonathan 
Published: April 2021

Abstract
Coupling interval (CI), the time (ms) from the onset of a sinus QRS to the onset of the following premature ventricular complex (PVC), and their variability (CIV) might predict mortality and elucidate mechanisms of arrhythmogenesis. There has been limited investigation of CIV in dogs. Therefore, we determined CIV and prematurity index (PI) in three groups of dogs with ventricular arrhythmias that were subject to 24 hour ambulatory electrocardiographic (Holter) monitoring. Dogs in group 1 had presumptive arrhythmogenic right ventricular cardiomyopathy (ARVC), those in group 2 had structural heart disease in which patients with valvular heart disease predominated, and those in group 3 had a dilated cardiomyopathy (DCM) either phenotype or presumed familial cardiomyopathy. In this preliminary study, we did not find significant differences in indices of CIV between groups. Median PI was lower in dogs treated with antiarrhythmic therapy. Severity of cardiac remodeling, except for left atrial to aortic ratio, were not correlated with CIV. It was not possible to determine the mechanism of arrhythmias in ARVC, DCM phenotype or structural heart disease groups and re-entry, triggered activity, and abnormal automaticity are possible etiologies. The effect of antiarrhythmic therapy demonstrated potential drug effect on CIV. Risk for malignant arrhythmias and sudden cardiac death were not examined. A larger study would be needed to determine if differences exist; if present, this would give insight into possible mechanisms and optimal antiarrhythmic therapy.
The axial load-share ratio in vivo as a supplemental assessment for the external fixator safe removal
Authors: Liu Yanshi , Jia Qiyu , Cai Feiyu , Liu Kai , Zhang Xingpeng , Li Hong , Zhang Tao , Yusufu Aihemaitijiang 
Published: April 2021

Abstract
Background: Timing the fixator removal is vital for a successful external fixation treatment. The purpose of this study was to determine the effectiveness of axial load-share ratio in vivo as a supplemental decision support tool for the safe removal of an Ilizarov external fixator.Methods: This prospective observational study consists of 83 patients undergoing tibial or femoral lengthening with Ilizarov circular external fixation in our institution, from January 2011 to October 2019. In group Ⅰ (38 patients), the external fixator was removed based on the surgeon’s clinical experience and radiographs from January 2011 to June 2015. In group Ⅱ (45 patients), from July 2015 to October 2019, the supplemental axial load-share (LS) ratio test was accomplished without the knowledge of the clinical results by another medical team. When the LS ratio < 10% was consistent with the conclusion (dense bone formation was achieved in the distraction zone) drawn from the corresponding routine radiographs by the treating surgeon, the external fixator was removed.Results: There was no statistical significance in demographic data between the two groups (P>0.05). In group Ⅰ, 4 of the 38 patients suffered refracture (the refracture rate was 10.5%) after fixator removal, and bone union was finally achieved with further intervention by intramedullary nail. In group Ⅱ, 36 patients terminated the external fixation after the first mechanical test, and another 9 patients terminated the external fixation at the subsequent test. None of the 45 patients in group Ⅱ suffered refracture (the refracture rate was 0%). There was statistical significance in the refracture rate between the two groups (P<0.05).Conclusions: Adequate assessment of bone regenerate is crucial before removing an external fixator to prevent deformation or refracture. The axial load-share ratio in vivo is a practically quantitative method to supplement radiography and clinical experience for the assessment of regenerate healing, and the axial load-share ratio dropped below 10% is a safe limit for the Ilizarov external fixator removal.
Vitamin D Deficiency and Depression in Obese Adults: A Case- Control Study
Authors: Kamalzadeh Leila , Jolfaei Atefeh Ghanbari , Saghafi Malihe 
Published: April 2021

Abstract
Background: Depression is one of the most prevalent psychiatric disorders reported in obese population. Amongst the contributing factors of depression, vitamin D deficiency has increasingly drawn attention in recent years. This paper seeks to examine the association between serum vitamin D level and depression in patients with obesity.Methods: This case-control study included 173 depressed obese patients and 174 non-depressed controls. Structured Clinical Interview for DSM-5 (SCID-5) was used to confirm the diagnosis of depression. 25-Hydroxyvitamin D [25(OH)D], Thyroid stimulating hormone (TSH), fasting blood sugar (FBS), parathyroid hormone (PTH) levels, and BMI were assessed in both groups. The statistical analyses included T-test, Chi-squared test, and multivariable logistic regression.Results: The mean 25(OH)D levels were significantly different between the case and control groups (20.43 ± 15.37 vs. 26.55 ± 13.17, P < .001). Vitamin D insufficiency/deficiency was detected in 77.6% and 67.4% of the case and control groups, respectively, which was significantly different (P = .034). Being female, greater age and lower vitamin D levels were associated with greater odds of developing depression (OR = 3.57, 95% CI = 1.82-7.02; OR = 1.05, 95% CI = 1.02-1.07; OR = 1.51, 95%CI = 1.16-1.96, respectively).Conclusion: The present study provides additional evidence with respect to the hypothesis that low vitamin D serum concentration is associated with depression in obese adults, and highlights the need for further research to determine whether this association is causal.
Colon Squamous Cell Carcinoma Hidden Under a Small Polyp: a Case Report
Authors: Ji Wen , Chen Weiqing 
Published: April 2021

Abstract
we report a case of colon squamous cell carcinoma incidentally diagnosed based on endoscopic polypectomy. Primary squamous cell carcinomas of the colon and rectum are extremely rare. This is not only a rare case, from this case we need to rethink how to choose the most appropriate polypectomy method.
Correlation Of SOX17, MACC1,&nbsp;c-Met With Clinicopathological Features And Prognosis of Esophageal Squamous Cell Carcinoma
Authors: Shi Yan , Li Chao , Li Mengyan , Abulajiang Gulinaer , Wang Hui , Su Liping , Liu Wenying , Xu Shanshan , Zhang Wenjing , Ma Yuqing 
Published: April 2021

Abstract
Aim: We mainly investigated the relationship between the expression of SOX17, MACC1 and c-Met in esophageal squamous cell carcinoma and the clinicopathological parameters of the patients and the effect on prognosis.Methods：Expressions of SOX17, MACC1 and c-Met protein in a sample of 232 ESCC and adjacent nontumorous tissues were detected by immunohistochemistry. Their relationships and correlations with clinicopathological features and clinical prognosis were analyzed by SPSS 23.0.Results：SOX17 was associated with Vascular invasion (p=0.017) and Nerve invasion (p=0.014). MACC1 was correlated with Tumor size (p=0.039) and TNM stage (p=0.020).c-Met was significantly associated with hematogenous metastasis (p=0.045). The expression of MACC1 was correlated with c-Met (P<0.001). c-Met expression(p=0.021) were associated with OS. Conclusion: SOX17, MACC1 and c-Met may be new diagnostic targets for esophageal squamous cell carcinoma. c-Met may be a new therapeutic and prognostic target.
Post-acute COVID-19 syndrome: Prolonged effort intolerance after SARS-CoV-2 infection
Authors: Duyan Murat , Savcıoğlu Ahmet Serbülent , Ozturan Ibrahim Ulas 
Published: April 2021

Abstract
Many new complications have been arising after being infected with SARS-CoV-2. Post-acute COVID-19 syndrome is a new definition for patients with persistent symptoms and prolonged complications beyond 4 weeks of acute COVID-19. In this report, we present two cases of prolonged dyspnea and reduced effort capacity for 4 and 5 months, respectively. A 29-year-old male and 40-year-old female patients, found to be positive in rRT-PCR tests for SARS-CoV-2, had recurrent admission to the emergency department after having treated for COVID-19. Both of the patients were followed for 6 months. Although all potential cardiovascular, pulmonary and other system pathologies were ruled out, the prolonged dyspnea and reduced effort capacity started to improve after 4 months in the female and 5 months in the male patients. The patients were diagnosed with post-acute COVID-19 syndrome. It should be kept in mind that the post-acute COVID-19 syndrome may be a complication in patients with persistent symptoms after COVID-19.
Prevalence of school absenteeism during menstruation and associated factors in sub-Saharan Africa: A systematic review and meta-analysis protocol
Authors: Gelo Amerti , Damena Melake , Geleto Ayele , Kenay Abera 
Published: April 2021

Abstract
Background: Although menstruation is a normal physiological process, it could affect several females’ daily activities. In most low resource settings, where menstrual hygiene management is poor and several schools have no water supply and separate toilet for female students, most school girls miss classes during menstruation. With the emergence of studies on menstrual hygiene management, it is evident that pooling several pocket studies is essential to draw lessons about effects of menstruation on female students’ class participation. Therefore, the aim of this review is to systematically assess the prevalence of school absenteeism and associated factors during menstruation among high school students in sub-Saharan Africa.Methods: The protocol for this systematic review was registered in PROSPERO (ID CRD42020223447). Electronic databases such as MEDLINE, CINAHL, Embase, Web of Science, Scopus and Google Scholar will be searched for published articles using appropriate key terms. The identified articles will be assessed for meeting the eligibility criteria such as being published in English, conducted in sub-Saharan Africa, contain information on school absenteeism, and conducted in schools. Two reviewers will independently screen the identified articles using titles and abstracts followed by full text review of all potential articles. The quality of the included papers will be assessed using the adapted Newcastle-Ottawa Scale for cross-sectional studies. The Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) checklist will be employed to present the findings. Quantitative findings will be pooled using random effect meta-analysis model while the qualitative findings will be presented using narrative synthesis.Discussion: Analysis of existing evidence of school absenteeism during menstruation is essential for designing appropriate interventions in school and beyond. This review will present credible information that would help in designing evidence-based menstrual hygiene management for improving school attendance during menstruation in sub-Saharan Africa where more than half of female students miss classes during menstruation.Systematic review registration: PROSPERO CRD42020223447
Dexibuprofen ameliorates peripheral and central risk factors associated with Alzheimer’s disease in metabolically stressed APPswe/PS1dE9 mice
Authors: Ettcheto Miren , Sánchez-Lopez Elena , Cano Amanda , Carrasco Marina , Herrera Katherine , Manzine Patricia R. , Espinosa-Jimenez Triana , Busquets Oriol , Verdaguer Ester , Olloquequi Jordi , Auladell Carme , Folch Jaume , Camins Antoni 
Published: April 2021

Abstract
Background: Several studies stablished a relationship between metabolic disturbances and Alzheimer´s disease (AD) where inflammation plays a pivotal role. However, mechanisms involved still remain unclear. In the present study, we aimed to evaluate central and peripheral effects of dexibuprofen (DXI) in the progression of AD in APPswe/PS1dE9 (APP/PS1) female mice, a familial AD model, fed with high fat diet (HFD). Animals were fed either with conventional chow or with HFD, from their weaning until their sacrifice, at 6 months. Moreover, mice were divided into subgroups to which were administered drinking water or water supplemented with DXI (20mg·kg-1·d-1) for 3 months. Before sacrifice, body weight, intraperitoneal glucose and insulin tolerance test (IP-ITT) were performed to evaluate peripheral parameters and also behavioral tests to determine cognitive decline. Moreover, molecular studies such as Western blot and RT-PCR were carried out in liver to confirm metabolic effects and in hippocampus to analyze several pathways considered hallmarks in AD. Results: Our studies demonstrate that DXI improved metabolic alterations observed in transgenic animals fed with HFD in vivo, data in accordance with those obtained at molecular level. Moreover, an improvement of cognitive decline and neuroinflammation among other pathways associated with AD were observed such as beta-amyloid plaque accumulation and unfolded protein response.Conclusions: Collectively, evidence suggest that chronic administration of DXI prevents the progression of AD through the regulation of inflammation which contribute to improve hallmarks of this pathology. Thus, this compound could constitute a novel therapeutic approach in the treatment of AD in a combined therapy.
Effectiveness of empirical anti-pseudomonal antibiotics in patients with recurrent COPD exacerbation: A multicenter retrospective cohort study
Authors: Shiroshita Akihiro , Miyakoshi Chisato , Tsutsumi Shunta , Shiba Hiroshi , Shirakawa Chigusa , Sato Kenya , Matsushita Shinya , Kimura Yuya , Tomii Keisuke , Ohgiya Masahiro , Kataoka Yuki 
Published: April 2021

Abstract
Background: Although frequent chronic obstructive pulmonary disease (COPD) exacerbation has been associated with the isolation of Pseudomonas aeruginosa (PA) in sputum cultures, it remains unknown whether the empirical use of anti-pseudomonal antibiotics can improve outcomes in patients with frequent COPD exacerbations. Research Question: Does empirical use of anti-pseudomonal antibiotics improve the length of the hospital stay in patients with recurrent COPD exacerbation?Study Design and Methods: This multicenter retrospective cohort study was conducted in seven hospitals in Japan. We included patients aged ≥40 years who were admitted to the hospital with COPD exacerbation more than twice during the study period (April 1, 2008 to July 31, 2020). The primary outcome was the length of the hospital stay. For statistical analysis, a log-linked Gamma model was used. Parameters were estimated using a generalized estimating equation model with an exchangeable correlation structure accounting for repeated observations from a single patient. Covariates included age, body mass index, home oxygen therapy use, respiratory rate, heart rate, oxygen use on admission, mental status, systemic steroid use, activities of daily living, and number of recurrences. Hospital-specific effects were specified as fixed effects.Results: Among 1573 patients with COPD exacerbation, 344 patients and 965 observations of recurrent COPD exacerbations were selected. Anti-pseudomonal antibiotics were used in 173 patients (18%). The estimated change in the length of the hospital stay between the anti-pseudomonal and non-anti-pseudomonal antibiotics groups was 0.044 days [95% confidence interval; -0.077, 0.166]. Interpretation: Use of empirical anti-pseudomonal antibiotics based only on recurrence may not be necessary. Further large-scale studies are needed to more precisely evaluate the effectiveness of empirical anti-pseudomonal antibiotics.
Radial Head Arthroplasty: Does Ligaments Repair Influence Outcomes? A Minimum Two Years Follow-Up Radiographic Multi-Center Study
Authors: Galavotti Cristina , Padovani Sara , Nosenzo Alessandro , Menozzi Margherita , Maniscalco Pietro , Concari Galeazzo , Villani Clemente , Sabetta Ettore , Belluati Alberto , Monesi Mauro , Massari Leo , Pari Carlotta , Cavaciocchi Michele , Calderazzi Filippo , Colozza Alessandra 
Published: April 2021

Abstract
Purpose: The current indication for comminuted radial head fractures is radial head arthroplasty (RHA). The main purpose of this study was to investigate any statistical differences in terms of prosthesis revision or removal and radiographic-degenerative changes by comparing patients who underwent RHA and ligaments repair to those who underwent only RHA implant at minimum two-years follow up. The secondary aim was to delineate a trend profile of RHA implants.Methods: All patients who underwent RHA surgery for traumatic pathology between January 2012 and December 2017 were eligible. Two researchers independently and retrospectively reviewed the patients’ charts and collected the following data: type of prosthesis, associated surgical procedures and revision surgery. They also looked for any radiographic sign of prosthesis loosening, overstuffing, capitellar osteopenia, heterotopic ossification and degenerative changes. No clinical evaluation was performed. Results: In six years 124 RHA were implanted (74 Female, 50 Male, mean age 56). The main diagnoses were: terrible-triad, trans-olecranon fracture and isolated radial head fracture. It was found no significant statistical difference between the two groups, nevertheless the cohort of patients that underwent ligaments repair had a lower revision rate in comparison to the other. Suture of the annular ligament seems to be critical. The overall revision rate was 10.5%.Conclusion:This multi-center study found no evidence that ligaments repair, as an associated surgical procedure, improves RHA longevity, except for annular ligament. Nevertheless, it seems to prevent degenerative changes at mid-term follow-up.
Predictive modelling of the stage–discharge relationship using Gene-Expression Programming
Authors: Birbal Prashant , Azamathulla Hazi , Leon Lee , Kumar Vikram , Hosein Jerome 
Published: April 2021

Abstract
Modelling the hydrologic processes is an essential tool for the efficient management of water resource systems. Therefore, researchers are consistently developing and improving various predictive/forecasting techniques to accurately represent a river's attributes, even though traditional methods are available. This paper presents the Gene-Expression Programming (GEP) modelling technique to accurately model the stage–discharge relationship for the Arouca River in Trinidad and Tobago using only low flow data. The proposed method uses the stage and associated discharge measurements at one cross-section of the Arouca River. These measurements were used to train the GEP model. The results of the GEP model were also compared to the traditional method of the Stage–Discharge Rating Curve (SRC). Four statistical paraments namely the Pearson's Correlation Coefficient (R), Root Mean Square Error (RMSE), Mean Absolute Relative Error (MARE) and Nash–Sutcliffe Efficiency (NSE) were used to evaluate the performance of the GEP model and the SRC method. Overall, the GEP model performed exceptionally well with an R2 of 0.990, RMSE of 0.104, MARE of 0.076 and NSE of 0.957.