bluuebunny/crossref_metadata_2025_split huggingface dataset:
Hubungan Tingkat Pengetahuan Dokter Gigi Muda dengan Tindakan Penggunaan Alat Pelindung Diri di RSIGM UMI Tahun 2018
Authors: Arifin Nur Fadhilah , Aslan Sarahfin , Selviani Yusrini , Fairuz Andy , Arifin Fadil Abdillah , Hilyah Hilyah 
Published: April 2021

Latar belakang: Infeksi merupakan bahaya yang sangat nyata pada praktik pelayanan kedokteran gigi. Pada kenyataannya, prosedur kebersihan tangan merupakan komponen paling penting diantara program pencegahan dan pengendalian infeksi. Dalam menjalankan profesinya, dokter gigi bukan tidak mungkin berkontak secara langsung ataupun tidak langsung dengan mikroorganisme dalam saliva dan darah pasien. Kedokteran gigi merupakan salah satu bidang yang rawan untuk terjadinya kontaminasi silang antara pasien-dokter gigi, pasien-pasien,dan pasien-perawat.Tindakan pertama pencegahan infeksi silang adalah pemakaian pelindung oleh operator misalnya masker, sarung tangan,dan kacamata pelindung yang memiliki standar yang bersifat proteksi,murah, dan secara universal digunakan pada dental surgeries sebagai barrier yang efektif. Tujuan: Untuk mengetahui tentang hubungan pengetahuan dokter gigi muda dengan penggunaan alat pelindung diri di RSIGM UMI. Metode: penelitian ini menggunakan jenis penelitian bersifat observasional analitik dengan menggunakan pendekatan cross sectional study. Sampel penelitian ini adalah dokter gigi muda di RSIGM Fakultas Kedokteran Gigi UMI Hasil: Sebagai dokter gigi muda memiliki tingkat pengetahuan yang baik yaitu sebanyak 76,7%, tingkat pengetahuan cukup yaitu sebanyak 16,7%, dan tingkat pengetahuan kurang yaitu sebanyak 6,7% dengan tindakan dokter gigi muda tentang penggunaan alat pelindung diri terhadap pencegahan penularan ifeksi silang di RSIGM UMI memiliki tindakan baik yaitu sebanyak 56,7%, tindakan cukup yaitu sebanyak 30%, dan tindakan kurang sebanyak 13,3%. Berdasarkan hasil pengujian hipotesis dengan analisis Chi Square menunjukan nilai p = 0.191 (p ? ? = 0.005). Kesimpulan: Tidak terdapat hubungan tingkat pengetahuan dokter gigi muda dengan penggunaan alat pelindung diri terhadap pencegahan penularan infeksi silang di RSIGM UMI tahun 2018.
Cellular uptake of nanoparticles: Involvement of caveolae?
Authors: Skotland Tore , Iversen Tore Geir , Sandvig Kirsten 
Published: April 2021

<img src=” https://s3.amazonaws.com/production.scholastica/article/22201/large/1416290_background_image.jpg?1618497820”> Here we discuss some pitfalls and challenges briefly when investigating which endocytic mechanisms are involved in the cellular uptake of nanoparticles. We specifically discuss some common misunderstandings regarding studies claimed to demonstrate uptake via caveolae. Scientists in the nanomedicine field should be aware that reducing the membrane content of cholesterol by adding methyl-β-cyclodextrin removes caveolae and inhibits other uptake mechanisms, such as macropinocytosis as well. Furthermore, the general tyrosine kinase inhibitor genistein is not a specific inhibitor of uptake from caveolae. Moreover, one can still see that scientists in the field write that they want to direct transport of their particles to caveolae and caveosomes to avoid lysosomal degradation. However, caveosomes are artifacts caused by overexpression of caveolin-1 constructs, and ligands or particles taken up by caveolae are transported to endosomes and lysosomes as reported for other types of endocytosis.
PENGEMBANGAN MODUL BERBASIS NILAI KARAKTER UNTUK MENINGKATKAN HASIL BELAJAR SISWA KELAS IV TEMA 7 SUBTEMA 3
Authors: Ifa Khoirotul , Muslihasari Adzimatnur 
Published: April 2021

Permasalahan penelitian ini beranjak dari data penelitian yang menunjukkan materi pembelajaran tematik masih sangat terbatas. Bahan ajar berupa modul belum pernah digunakan dalam proses pembelajaran karena masih menggunakan buku paket tematik siswa serta materi pembelajaran dan konten bacaan dalam buku paket terkesan monoton. Dalam penelitian ini, mengembangkan bahan ajar berupa modul tematik berbasis nilai- nilai karakter yang layak dan valid untuk digunakan sebagai bahan ajar dan dapat meningkatkan hasil belajar pada siswa. Penelitian ini merupakan penelitian pengembangan atau Research and Development (R&D) yang mengacu pada model ADDIE yang terdiri dari lima tahap: (1)analysis; (2) design; (3) development; (4) implementation; (5) Evaluation. Subjek dalam penelitian ini adalah kelompok terbatas dari siswa kelas IV SDN Pronojiwo 04 yang berjumlah 10 siswa. Teknik Pengumpulan data dilakukan dengan angket atau kuesioner dan pengukuran hasil belajar siswa. Hasil dari penelitian Pengembangan Modul Tematik Berbasis Nilai- Nilai Karakter menunjukkan layak digunakan sebagai bahan ajar. Hal ini didasarkan pada skor penilaian yang diperoleh melalui skor penilaian dari ahli materi memperoleh persentase 81% dengan kriteria sangat layak, skor penilaian dari wali kelas IV memperoleh persentase 83% dengan kriteria sangat layak dan hasil dari respon siswa terhadap bahan ajar modul berbasis nilai- nilai karakter 91% dengan kriteria sangat layak. Hal ini dapat dikatakan bahwa media sangat layak dan valid digunakan dalam meningkatkan hasil belajar siswa, ditunjukkan dengan persentase peningkatan hasil belajar mencapai 80%.
Continuous Classification of Locomotion in Response to Task Complexity and Anticipatory State
Authors: Kazemimoghadam Mahdieh , Fey Nicholas P. 
Published: April 2021

ObjectiveIntent recognition in lower-extremity assistive devices (e.g., prostheses and exoskeletons) is typically limited to either recognition of steady-state locomotion or changes of terrain (e.g., level ground to stair) occurring in a straight-line path and under anticipated condition. Stability is highly affected during non-steady changes of direction such as cuts especially when they are unanticipated, posing high risk of fall-related injuries. Here, we studied the influence of changes of direction and user anticipation on task recognition, and accordingly introduced classification schemes accommodating such effects.MethodsA linear discriminant analysis (LDA) classifier continuously classified straight-line walking, sidestep/crossover cuts (single transitions), and cuts-to-stair locomotion (mixed transitions) performed under varied task anticipatory conditions. Training paradigms with varying levels of anticipated/unanticipated exposures and analysis windows of size 100–600 ms were examined.ResultsMore accurate classification of anticipated relative to unanticipated tasks was observed. Including bouts of target task in the training data was necessary to improve generalization to unanticipated locomotion. Only up to two bouts of target task were sufficient to reduce errors to &lt;20% in unanticipated mixed transitions, whereas, in single transitions and straight walking, substantial unanticipated information (i.e., five bouts) was necessary to achieve similar outcomes. Window size modifications did not have a significant influence on classification performance.ConclusionAdjusting the training paradigm helps to achieve classification schemes capable of adapting to changes of direction and task anticipatory state.SignificanceThe findings could provide insight into developing classification schemes that can adapt to changes of direction and user anticipation. They could inform intent recognition strategies for controlling lower-limb assistive to robustly handle “unknown” circumstances, and thus deliver increased level of reliability and safety.
Macro and Trace Elements in Hemp (Cannabis sativa L.) Cultivated in Greece: Risk Assessment of Toxic Elements
Authors: Zafeiraki Effrosyni , Kasiotis Konstantinos M. , Nisianakis Paul , Machera Kyriaki 
Published: April 2021

The accumulation of hazardous contaminants in Cannabis sativa L. raises warning signs regarding possible adverse effects on human health due to the consumption of herbal medicines and/or other herbal edible products made from cannabis. Thus, there is an urge to investigate the levels of hazardous contaminants, such as heavy metals, in cannabis plant. In the present study, 29 macro and trace elements, including both beneficial and toxic elements (heavy metals and metalloids), were investigated in 90 samples of Cannabis sativa L. collected from Greece. According to the results, the detected concentrations of macro elements in the leaves/flowers of cannabis ranged between 28 and 138,378 ppm, and of trace elements between 0.002 and 1352.904 ppm. Although the concentrations of elements varied among the samples, their accumulation pattern was found to be similar, with the contribution of toxic elements to the total concentration of trace elements being below 1%. The detected levels of the most toxic elements were below the prescribed limits established by the WHO, while the calculated THQ and CR values showed no risk (non-carcinogenic and carcinogenic) for the population exposed to the current cannabis samples. Positive correlation between the concentration of elements and cannabis geographical origin and variety was observed. Cannabis leaves/flowers were more contaminated with trace and macro elements than seeds.
A Rapid Intraoperative Parathyroid Hormone Assay Based on the Immune Colloidal Gold Technique for Parathyroid Identification in Thyroid Surgery
Authors: Xia Wenfei , Zhang Jinjun , Shen Wenzhuang , Zhu Zhi , Yang Zhifang , Li Xingrui 
Published: April 2021

ObjectiveA novel immunochromatographic test strip method was developed to detect tissue parathyroid hormone (PTH) using the immune colloidal gold technique (ICGT). The accuracy and application value of this method for intraoperative parathyroid identification were evaluated.MethodsSerum samples were collected to measure PTH by both ICGT and electrochemiluminescence immunoassay (ECLIA). Patients who underwent unilateral and total thyroidectomy were enrolled to evaluate the feasibility and clinical efficacy of rapid intraoperative identification of parathyroid glands via PTH determination using ICGT. Two sample preparation methods, fine needle aspiration (FNA) and tissue block homogenate (TBH), were used for PTH-ICGT analysis.ResultsBablok analysis showed a linear relationship between the serum PTH measurements obtained by ICGT and ECLIA. Non-parathyroid tissues had much lower PTH concentrations (14.8 ± 2.1 pg/ml, n = 97) detected by ICGT, compared to the parathyroid gland tissues (955.3 ± 16.1 pg/ml, n = 79; P &lt; 0.0001), With biopsy results as the standard, ICGT showed higher diagnosis rates as compared with direct visual inspection, for identifying both parathyroid glands (97.4 vs. 78.2%) and non-parathyroid tissues (100 vs. 68.9%). The cut-off values for parathyroid identification by FNA and TBH methods were 63.99 and 136.30 pg/ml, respectively. The detection time was 2 min by TBH method for in vitro tissue detection and 6 min by FNA method for in situ tissue detection, both of which were faster than traditional intraoperative cryopathological examination (usually &gt;30 min). Intraoperative application of ICGT method was associated with higher postoperative serum calcium and blood PTH levels at 1 and 3 months as well as a lower incidence of postoperative transient hypocalcemia, as compared with direct visual inspection.ConclusionPTH-ICGT assay shows high potential as a rapid, novel alternative for intraoperative parathyroid identification.
Effect of Additional Amino Group to Improve the Performance of Immobilized Lipase From Aspergillus niger by Adsorption-Crosslinking Method
Authors: Nuraliyah Andi , Perdani Meka Saima , Putri Dwini Normayulisa , Sahlan Muhamad , Wijanarko Anondho , Hermansyah Heri 
Published: April 2021

Adsorption-crosslinking is one of the immobilization methods to improve the reusability of lipase. It requires amino groups to reduce cross-link immobilization risk so that lipase–support interaction increases and the immobilization is attainable. Also, the amino group on the support is expected to increase lipase performance. This study aimed to analyze the effect of amino group addition on immobilized Aspergillus niger lipase by the adsorption-crosslinking using MP-64 macroporous anion resin and XAD-7HP macroporous nonionic resin that has been treated with chitosan. The chitosan-coated resin was characterized by Fourier transform infrared spectrometry (FTIR) and scanning electron microscope (SEM). Lipase immobilization was carried out by adding 10 ml lipase solution containing 0.75 g resins and shaken at 25°C for 150 rpm. Adsorption was achieved for 4 h, followed by cross-linking separately (adding 0.5% (v/v) glutaraldehyde and re-reacting for 20 min). Lipase activity was measured with the titrimetric of olive oil emulsion; mixed with Aspergillus niger lipase, emulsion, and a buffer solution (pH 6.5, ionic strength of 0.7); and incubated for 30 min at 37°C. The effect of amino-functional groups was investigated based on lipase loading and lipase activity. The best lipase loading and lipase activity of 83.79% and 29.41 U/g support were achieved in the adsorption-crosslinking using MP-64 resin coated with chitosan. After four cycles, biodiesel synthesis was maintained at 70.61% of the initial yield. These results indicated that chitosan as an affordable and readily available source of amino groups could be used to modify support for Aspergillus niger lipase immobilization.
Role of CCR2+ Myeloid Cells in Inflammation Responses Driven by Expression of a Surfactant Protein-C Mutant in the Alveolar Epithelium
Authors: Venosa Alessandro , Cowman Sophie , Katzen Jeremy , Tomer Yaniv , Armstrong Brittnie S. , Mulugeta Surafel , Beers Michael F. 
Published: April 2021

Acute inflammatory exacerbations (AIE) represent precipitous deteriorations of a number of chronic lung conditions, including pulmonary fibrosis (PF), chronic obstructive pulmonary disease and asthma. AIEs are marked by diffuse and persistent polycellular alveolitis that profoundly accelerate lung function decline and mortality. In particular, excess monocyte mobilization during AIE and their persistence in the lung have been linked to poor disease outcome. The etiology of AIEs remains quite uncertain, but environmental exposure and genetic predisposition/mutations have been identified as two contributing factors. Guided by clinical evidence, we have developed a mutant model of pulmonary fibrosis leveraging the PF-linked missense isoleucine to threonine substitution at position 73 [I73T] in the alveolar type-2 cell-restricted Surfactant Protein-C [SP-C] gene [SFTPC]. With this toolbox at hand, the present work investigates the role of peripheral monocytes during the initiation and progression of AIE-PF. Genetic ablation of CCR2+ monocytes (SP-CI73TCCR2KO) resulted in improved lung histology, mouse survival, and reduced inflammation compared to SP-CI73TCCR2WT cohorts. FACS analysis of CD11b+CD64-Ly6Chi monocytes isolated 3 d and 14 d after SP-CI73T induced injury reveals dynamic transcriptional changes associated with “Innate Immunity’ and ‘Extracellular Matrix Organization’ signaling. While immunohistochemical and in situ hybridization analysis revealed comparable levels of tgfb1 mRNA expression localized primarily in parenchymal cells found nearby foci of injury we found reduced effector cell activation (C1q, iNOS, Arg1) in SP-CI73TCCR2KO lungs as well as partial colocalization of tgfb1 mRNA expression in Arg1+ cells. These results provide a detailed picture of the role of resident macrophages and recruited monocytes in the context of AIE-PF driven by alveolar epithelial dysfunction.
Influence of Specimen Size on Autogenous Volume Deformation of Long-Aged MgO-Admixed Concrete
Authors: Chen Changli , Chen Rongfei , Zhao Zhenhua , Li Weiwei , Yan Shaolian , Du Xiangqin , Yang Huashan 
Published: April 2021

The transformation law of autogenous volume deformation of MgO-admixed concrete with specimen size was revealed through continuous observation on standard-size (Φ200 mm × 500 mm), medium-size (Φ250 mm × 500 mm), and large-size specimens (Φ250 mm × 600 mm) over 6 years. Besides, the pore parameters of concrete core samples obtained from autogenous volume deformation specimens in the 1st, 3rd, and 6th years of age were investigated. The results show that the autogenous volume deformation of MgO-admixed concrete increases with the increase in the MgO content or age. The expansion rate of the concrete specimen decreases after the age of 360 days, and the autogenous volume expansion deformation of the specimen tends to be stable after about 2 years. When the size of the specimen changes, the autogenous volume deformation of MgO-admixed concrete decreases with the increase of specimen size. During the age of 2–6 years, the expansion of medium- and large-size specimens is reduced by 6–10 and 15–20%, respectively, compared with the standard-size specimens under the same MgO content. With the condition of an appropriate MgO content, regardless of the size of the specimen, the pore structure of the concrete becomes better and better with the growth of age, the concrete becomes denser and denser, and the expansion caused by MgO hydration will not cause damage to concrete structures.
Pan-Cancer Analysis of FURIN as a Potential Prognostic and Immunological Biomarker
Authors: Zhou Bolun , Gao Shugeng 
Published: April 2021

BackgroundFurin is a calcium-dependent protease that processes various precursor proteins through diverse secretory pathways. The deregulation of FURIN correlated with the prognosis of patients in numerous diseases. However, the role of FURIN in human pan-cancer is still largely unknown.MethodsMultiple bioinformatic methods were employed to comprehensively analyze the correlation of FURIN expression with prognosis, mismatch repair (MMR), microsatellite instability (MSI), tumor mutational burden (TMB), DNA methylation, tumor immune infiltration, and common immune checkpoint inhibitors (ICIs) from the public database, and aim to evaluate the potential prognostic value of FURIN across cancers.ResultsFURIN was aberrantly expressed and was strongly correlated with MMR, MSI, TMB, and DNA methylation in multiple types of cancer. Moreover, survival analysis across cancers revealed that FURIN expression was correlated with overall survival (OS) in four cancers, disease-specific survival (DSS) in five cancers, progression-free interval (PFI) in seven cancers, and disease-free interval (DFI) in two cancers. Also, FURIN expression was related to immune cell infiltration in 6 cancers and ImmuneScore/StromalScore in 10 cancers, respectively. In addition, FURIN expression also showed strong association between expression levels and immune checkpoint markers in three cancers.ConclusionFURIN can serve as a significant prognostic biomarker and correlate with tumor immunity in human pan-cancer.
TRIM68, PIKFYVE, and DYNLL2: The Possible Novel Autophagy- and Immunity-Associated Gene Biomarkers for Osteosarcoma Prognosis
Authors: Jiang Jie , Liu Dachang , Xu Guoyong , Liang Tuo , Yu Chaojie , Liao Shian , Chen Liyi , Huang Shengsheng , Sun Xuhua , Yi Ming , Zhang Zide , Lu Zhaojun , Wang Zequn , Chen Jiarui , Chen Tianyou , Li Hao , Yao Yuanlin , Chen Wuhua , Guo Hao , Liu Chong , Zhan Xinli 
Published: April 2021

IntroductionOsteosarcoma is among the most common orthopedic neoplasms, and currently, there are no adequate biomarkers to predict its prognosis. Therefore, the present study was aimed to identify the prognostic biomarkers for autophagy-and immune-related osteosarcoma using bioinformatics tools for guiding the clinical diagnosis and treatment of this disease.Materials and MethodsThe gene expression and clinical information data were downloaded from the Public database. The genes associated with autophagy were extracted, followed by the development of a logistic regression model for predicting the prognosis of osteosarcoma using univariate and multivariate COX regression analysis and LASSO regression analysis. The accuracy of the constructed model was verified through the ROC curves, calibration plots, and Nomogram plots. Next, immune cell typing was performed using CIBERSORT to analyze the expression of the immune cells in each sample. For the results obtained from the analysis, we used qRT-PCR validation in two strains of human osteosarcoma cells.ResultsThe screening process identified a total of three genes that fulfilled all the screening criteria. The survival curves of the constructed prognostic model revealed that patients with the high risk presented significantly lower survival than the patients with low risk. Finally, the immune cell component analysis revealed that all three genes were significantly associated with the immune cells. The expressions of TRIM68, PIKFYVE, and DYNLL2 were higher in the osteosarcoma cells compared to the control cells. Finally, we used human pathological tissue sections to validate the expression of the genes modeled in osteosarcoma and paracancerous tissue.ConclusionThe TRIM68, PIKFYVE, and DYNLL2 genes can be used as biomarkers for predicting the prognosis of osteosarcoma.
Clinical Phenotypes of Tumors Invading the Rectosigmoid Colon Affecting the Extent of Debulking Surgery and Survival in Advanced Ovarian Cancer
Authors: Park Soo Jin , Mun Jaehee , Lee Eun Ji , Park Sunwoo , Kim Sang Youn , Lim Whasun , Song Gwonhwa , Kim Jae-Weon , Lee Seungmee , Kim Hee Seung 
Published: April 2021

We classified clinical phenotypes based on tumor separability from the rectosigmoid colon and then evaluated the effect of these clinical phenotypes on surgical outcomes and prognosis of advanced ovarian cancer. We collected data of patients with stage IIIB-IVB disease who either underwent visceral segmental serosectomy (VSS) or low anterior resection (LAR) during maximal debulking surgery. All patients were divided into the following, according to the resection types of tumors involving the rectosigmoid colon: the focal (tumor-involved length &lt;18 cm) and separable (FS) group that received VSS, the focal and inseparable (FI) that received LAR, or the diffuse (tumor-involved length ≥18 cm) group (D) that also received LAR. A total of 83 patients were included in FS (n=44, 53%), FI (n=18, 21.7%), and D (n=24, 25.3%) groups. FS and D groups with more extensive tumors were related to wider extent of surgery and more tumor infiltration except for bowels, whereas FI and D groups with more invasive tumors were associated with wider extent of surgery, more tumor infiltration to bowels, longer operation time, more blood loss, more transfusion, longer hospitalization, and higher surgical complexity scores. Moreover, FS and FI groups showed better progression-free survival than D group, whereas FS group demonstrated better overall survival than FI and D groups. Clinical phenotypes based on tumor separability from the rectosigmoid colon may depend on tumor invasiveness and extensiveness in advanced ovarian cancer. Moreover, these clinical phenotypes may affect surgical outcomes and survival.