bluuebunny/crossref_metadata_2025_split huggingface dataset:
KIF18B is a Prognostic Biomarker and Correlates with Immune Infiltrates in Pan-Cancer
Authors: Qiu Meng-jun , Wang Qiu-shuang , Li Qiu-ting , Zhu Li-sheng , Li Ya-nan , Yang Sheng-li , Xiong Zhi-fan 
Published: May 2021

Background: Cancer is one of the deadliest diseases at present. Although effective screening and treatment can save lives to a certain extent, our knowledge of the disease is far from sufficient. KIF18B is a member of the kinesin-8 superfamily and plays a conserved regulatory role in the cell cycle. KIF18B reportedly functions as an oncogene in some human cancers, but the correlations between KIF18B and prognosis and immune-infiltrates in different cancers remain unclear.Methods: Data were collected from the TCGA, GTEx, CCLE, TIMER, and GSEA databases. The expression difference, survival, pathological stage, DNA methylation, tumor mutation burden (TMB), microsatellite instability (MSI), mismatch repairs (MMRs), tumor microenvironment (TME), immune cell infiltration, and gene co-expression of KIF18B were analyzed using the R language software.Results: KIF18B was widely upregulated in cancers, compared with normal tissues, and high KIF18B expression was associated with unfavorable prognoses. TMB, MSI, MMRs, and DNA methylation correlated with KIF18B dysregulation in cancers. KIF18B correlated closely with tumor immunity and interacted with different immune cells and genes in different cancer types.Conclusion: KIF18B could be used as a prognostic biomarker for determining prognosis and immune infiltration in pan-cancer.
Lung Function After Stereotactic Body Radiation Therapy for Early-Stage Non-Small Cell Lung Cancer, Changes and Predictive Markers
Authors: Berg Janna , Ramberg Christina , Haugstvedt Jon Olav Sulheim , Bengtson May-Bente , Gabrielsen Anne-Marie , Brustugun Odd Terje , Halvorsen Ann Rita , Helland Åslaug 
Published: May 2021

IntroductionThe present study explores changes in pulmonary function, symptoms and radiological signs of pneumonitis after curatively intended stereotactic body radiation therapy (SBRT).MethodsAll inoperable, early-stage non-small cell lung cancer patients treated with stereotactic body radiation therapy (SBRT) from 2014-2017 were included in this single-centre study. They were followed regularly for 12 months after treatment. The patients were classified into three groups based on radiology and symptomatology: no radiation pneumonitis, asymptomatic and symptomatic radiation pneumonitis.ResultsForty-four patients with stage IA-IIB disease were treated with 45–56 Gy in 3–8 fractions. The median age was 75 years, 43% of the patients were female; 60% of the patients had a COPD in GOLD grade of 2-4, and 95.5% were active or former smokers. Symptomatic radiation pneumonitis occurred in 18% of the patients and asymptomatic pneumonitis as defined by radiology, in 39%. The mean of forced expiratory volume in 1 second (FEV1) and diffusion capacity for carbon monoxide (DLCO) decreases for all patients during the first years were higher than one would expect from physiologic ageing. FEV1 and DLCO in percent decrease 7-8% at 1-1.5 months in the symptomatic radiation pneumonitis group. CT scan findings consistent with radiation pneumonitis occurred after a median of 2.9 months in the symptomatic and 5.4 months in the asymptomatic radiation pneumonitis groups. In the group with symptomatic radiation pneumonitis, symptoms, as measured by the Clinical COPD questionnaire score, significantly increased at 3 and 6 months. Significant higher maximum doses to the critical lung volumes DC1000cm3(1000 cm3of lung receiving a given dose or less) and DC 1500cm3(1500 cm3of lung receiving a given dose or less) were observed in patients who developed radiation pneumonitis.ConclusionEarly decrease in measured FEV1 and DLCO occurred before imaging changes and symptoms and might indicate the development of symptomatic radiation pneumonitis. The dose to critical lung volumes of DC1000 cm3and DC1500 cm3may predict the risk for the development of symptomatic radiation pneumonitis.
Modulatory Effects of Bacillus subtilis on the Performance, Morphology, Cecal Microbiota and Gut Barrier Function of Laying Hens
Authors: Zhang Guangzhi , Wang Hao , Zhang Jianwei , Tang Xinming , Raheem Abdul , Wang Mingyan , Lin Weidong , Liang Lin , Qi Yuzhuo , Zhu Yali , Jia Yaxiong , Cui Shangjin , Qin Tong 
Published: May 2021

We investigated the efficacy of a single bacterium strain, Bacillus subtilis (B. subtilis) YW1, on the performance, morphology, cecal microbiota, and intestinal barrier function of laying hens. A total of 216 28-week-old Hy-line Brown laying hens were divided into three dietary treatment groups, with six replicates of 12 birds each for 4 weeks. The control group (Ctr) was fed a basal diet and the treatment groups, T1 and T2, were fed a basal diet supplemented with B. subtilis at a dose rate of 5 × 108 CFU/kg and 2.5 × 109 CFU/kg, respectively. Dietary supplementation with B. subtilis did not significantly affect overall egg production in both groups, with no obvious changes in average egg weight and intestine morphology. B. subtilis administration also improved the physical barrier function of the intestine by inducing significantly greater expression levels of the tight junction protein occludin in T1 (p = 0.07) and T2 (p < 0.05). Further, supplementation with B. subtilis effectively modulated the cecal microbiota, increasing the relative level of beneficial bacteria at the genus level (e.g., Bifidobacterium p < 0.05, Lactobacillus p = 0.298, Bacillus p = 0.550) and decreasing the level of potential pathogens (e.g., Fusobacterium p < 0.05, Staphylococcus p < 0.05, Campylobacter p = 0.298). Overall, B. subtilis YW1 supplementation cannot significantly improve the egg production; however, it modulated the cecal microbiota towards a healthier pattern and promoted the mRNA expression of the tight junction protein occludin in laying hens, making B. subtilis YW1 a good probiotic candidate for application in the poultry industry, and further expanding the resources of strains of animal probiotics.
Variational Quantum Chemistry Programs in JaqalPaq
Authors: Maupin Oliver G. , Baczewski Andrew D. , Love Peter J. , Landahl Andrew J. 
Published: May 2021

We present example quantum chemistry programs written with JaqalPaq, a python meta-programming language used to code in Jaqal (Just Another Quantum Assembly Language). These JaqalPaq algorithms are intended to be run on the Quantum Scientific Computing Open User Testbed (QSCOUT) platform at Sandia National Laboratories. Our exemplars use the variational quantum eigensolver (VQE) quantum algorithm to compute the ground state energies of the H2, HeH+, and LiH molecules. Since the exemplars focus on how to program in JaqalPaq, the calculations of the second-quantized Hamiltonians are performed with the PySCF python package, and the mappings of the fermions to qubits are obtained from the OpenFermion python package. Using the emulator functionality of JaqalPaq, we emulate how these exemplars would be executed on an error-free QSCOUT platform and compare the emulated computation of the bond-dissociation curves for these molecules with their exact forms within the relevant basis.
Entamoeba histolytica: Proteomics Bioinformatics Reveal Predictive Functions and Protein–Protein Interactions of Differentially Abundant Membrane and Cytosolic Proteins
Authors: Azmi Norhidayah , Othman Nurulhasanah 
Published: May 2021

Amoebiasis is caused by Entamoeba histolytica and ranked second for parasitic diseases causing death after malaria. E. histolytica membrane and cytosolic proteins play important roles in the pathogenesis. Our previous study had shown several cytosolic proteins were found in the membrane fraction. Therefore, this study aimed to quantify the differential abundance of membrane and cytosolic proteins in membrane versus cytosolic fractions and analyze their predicted functions and interaction. Previous LC-ESI-MS/MS data were analyzed by PERSEUS software for the differentially abundant proteins, then they were classified into their functional annotations and the protein networks were summarized using PantherDB and STRiNG, respectively. The results showed 24 (44.4%) out of the 54 proteins that increased in abundance were membrane proteins and 30 were cytosolic proteins. Meanwhile, 45 cytosolic proteins were found to decrease in abundance. Functional analysis showed differential abundance proteins involved in the molecular function, biological process, and cellular component with 18.88%, 33.04% and, 48.07%, respectively. The STRiNG server predicted that the decreased abundance proteins had more protein–protein network interactions compared to increased abundance proteins. Overall, this study has confirmed the presence of the differentially abundant membrane and cytosolic proteins and provided the predictive functions and interactions between them.
Synthesis of EPA- and DHA-Enriched Structured Acylglycerols at the sn-2 Position Starting from Commercial Salmon Oil by Enzymatic Lipase Catalysis under Supercritical Conditions
Authors: Dovale-Rosabal Gretel , Rodríguez Alicia , Espinosa Alejandra , Barriga Andrés , Aubourg Santiago P. 
Published: May 2021

There is consistent evidence that long-chain polyunsaturated fatty acids (LCPUFA) belonging to the n-3 series, i.e., eicosapentaenoic (20:5n-3, EPA) and docosahexaenoic (22:6n-3, DHA) acids, decrease the risk of heart, circulatory and inflammatory diseases. Furthermore, the bioavailability of such fatty acids has been shown to depend on their location in triacylglycerol (TG) molecules at the sn-2 position. Consequently, great attention has been accorded to the synthesis of structured acylglycerols (sAG), which include EPA or DHA at the sn-2 position. The aim of this work was to synthesize sAG starting from deodorized refined commercial salmon oil. For this, immobilized lipase B from Candida antarctica (nonspecific) was used as a catalyst for the intra–interesterification process under CO2 supercritical conditions (CO2SC). According to the CO2SC reaction time, three different fractions including sAG compounds were obtained. The location of EPA and DHA at the sn-2 position in the resulting glycerol backbone was identified by mass spectrometry (MALDI-TOF) analysis. In all fractions obtained, a marked decrease in the starting TG content was observed, while an increase in the DHA content at the sn-2 position was detected. The fraction obtained after the longest reaction time period (2 h) led to the highest yield of sn-2 position DHA in the resulting sAG molecule.
Formações como foco
Authors: Silva Judilma Aline , Rocha Paula Rejane Lisboa da 
Published: May 2021

Este artigo apresenta duas experiências de pesquisa envolvendo a formação docente da educação básica, superior e do gestor. O primeiro um recorte de uma pesquisa finalizada em 2019, de doutorado em uma Instituição de Ensino Superior pública. O objetivo foi mapear as ações institucionais voltadas para a formação dos docentes universitários, desenvolvidas pelas IES públicas federais no Brasil. O Segundo uma pesquisa que problematiza a gestão educacional por resultados implantados no Estado de Pernambuco, como também as suas implicações para o gestor. As pesquisas possuem a formação continuada como ponto desencadeador e o referencial teórico apresentado em cada discussão remete aos autores da contemporaneidade. Ambos os estudos apontam avanços processuais no entendimento sobre processos formativos.
Darwinowskie paradygmaty. Kultura popularna w poszukiwaniu teorii wszystkiego
Authors: Oramus Dominika 
Published: May 2021

The article attempts to prove that Darwinism in popular culture plays a role of a theory of everything. Bestselling authors of popular science such as Edward O. Wilson, Richard Dawkins and Bill Bryson have acquainted general public with the theory of evolution, and its newest facet — the Modern Synthesis. Darwinian paradigms, as defined by Thomas Kuhn, are also used in popular books on cosmology, sociobiology, psychology, and religious studies. Moreover, the Darwinian grand narrative of evolutional history shapes the way in which contemporary mass culture presents the history of our planet in numerous educational TV series. Last but not least, Charles Darwin himself has recently become a popular icon and the story of his life is remade in a growing number of fiction and non-fiction books and movies.
Stages of launching a biological treatment plant at the wastewater treatment facilities with dosing easily oxidable organic matter
Authors: Кондрашев В.А. , Метелица С.Г. 
Published: May 2021

Рассмотрены вопросы, связанные с проведением пусконаладочных работ биоблока станции очистки хозяйственно-бытовых и близких к ним по составу сточных вод при пробном пуске в эксплуатацию очистных сооружений. Подробно рассмотрены этапы пусконаладочных работ биоблока очистных сооружений с «затравкой» активным илом из действующих биологических очистных сооружений и с дозировкой легкоокисляемой органики. Описан состав оборудования станции КОС-9 производства «Гермес Групп». Рассмотрены все этапы пусконаладочных работ биоблока на примере запуска очистных сооружений КОС-9 с привлечением многочисленных опытных данных. Определены периоды этапов пусконаладки биоблока. Приведены проблемы наладки биоблока и пути их решения с достижением требуемого результата. Технология, используемая на станции, обеспечивает очистку сточных вод, соответствующую требованиям, предъявляемым к выпуску очищенных стоков в водоем рыбохозяйственного значения.
Issues related to commissioning a biological treatment plant at the facilities for household and similar in composition wastewater treatment during the trial start of the treatment facilities are considered. The stages of commissioning a biological treatment plant at the wastewater treatment facilities with «inoculating» activated sludge from the operating biological treatment facilities and with dosing easily oxidable organic matter are considered in detail. The equipment configuration of the WWTP-9 produced by Germes Group is described. All stages of the biological treatment plant commissioning are considered through the example of the start-up of WWTP-9 with the use of numerous experimental data. The periods of biological treatment plant commissioning stages have been determined. The problems of adjusting the biological treatment plant and the ways of their elimination to achieve the required result are presented. The technology used at the WWT facilities provides for the effluent quality that meets the requirements for the discharge into a water body of commercial fishing importance.
PROTOCOLO: COLOCACIÓN Y RETIRADA DEL EQUIPO DE PROTECCIÓN PERSONAL (EPP) PARA LA ATENCIÓN DE PACIENTES CON COVID-19. HOSPITAL REGIONAL LAMBAYEQUE
Authors: Villegas Cruz Pablo Donald , Cusman Aguilar Oscar Miguel , Vásquez Acosta Diana Patricia 
Published: May 2021

El virus Covid-19 se transmite a través de contacto y gotas por eso diferentes organizaciones y asociaciones de salud sugieren el uso adecuado del Equipo de protección Personal (EPP) para la atención de los pacientes con esta patología.
El objetivo principal de la elaboración del protocolo es disminuir la transmisión del virus Covid-19 desde cualquier fuente, asimismo uniformizar criterios sobre el manejo de los EPP teniendo en consideración los recursos que cuenta nuestro Hospital; además verificar la correcta colocación y retiro de los EPP a través de las listas de chequeos con el fin de disminuir o mitigar errores.
La lista de chequeo será ejecutada en el ingreso y salida del personal de salud asignada a laborar en el servicio para la atención del paciente con Covid19.
Efektifitas Penggunaan Games Edukasi untuk Meningkatkan Kemampuan dan Hasil Belajar Siswa dengan Aplikasi Mobile Learning pada Mata Kuliah Computer Programming
Authors: Patmah Fatoni  , Mira Rosalina  
Published: May 2021

Mata kuliah Computer Programming merupakan mata kuliah yang cukup banyak menyita waktu dalam menjelaskan model, kode sumber, rangkaian instruksi dan fungsi spesifik pada bahasa komputer, dan setiap mahasiswa membutuhkan konsentrasi agar dapat memahami dan mengerti mata kuliah computer programming dalam waktu satu semester. Namun mahasiswa mengalami kejenuhan sehingga isi mata kuliah tidak dapat tersampaikan dengan baik oleh karena itu penelitian ini menggunakan metode proses pembelajaran menggunakan games edukasi dalam satu semester. Bahasa pemrograman fungsional dan bahasa pemrograman logikal yang terdapat dalam mata kuliah computer programming dapat disampaikan melalui games edukasi yang memiliki operasi matematika dan sumber kode yang dibutuhkan dalam modus interaktif yang dapat diterjemahkan dan dieksekusi lebih mendalam dan sederhana.
Games edukasi ini merupakan media pembelajaran yang bersifat mendidik, mandiri dan merdeka dalam menjalankan pembelajaran, diharapkan mahasiswa dapat berpikir kreatif, inovatif dan produktif secara asyik dan menyenangkan. Metode penelitian pengambilan data menggunakan model pembelajaran mobile learning berupa tutorial, latihan praktek, penemuan dan simulasi pengambilan data. Dengan games edukasi aplikasi mobile learning, mahasiswa dapat meningkatkan kemampuan dan hasil belajar pada mata kuliah computer programming dengan melakukan metode penelitian secara kuantitatif berupa angka dan analisa menggunakan teknik statistika yang dapat dilihat dari hasil normalisasi gain yang diperoleh sebesar 0.52 atau 52% termasuk ke dalam kategori sedang atau sebagian besar mahasiswa mengalami peningkatan hasil belajar. Penggunaan games edukasi mobile learning cukup efektif digunakan untuk meningkatkan hasil belajar mahasiswa dilihat dari nilai normalisasi gain dan respon belajar mahasiswa dalam pencapaian pembelajaran yang terarah dan tercapai seluruh isi satuan pembelajaran mata kuliah computer programming.
Dengan meningkatnya hasil belajar yang diperoleh maka mahasiswa memiliki kepercayaan diri yang lebih, dapat merancang tujuan lebih detail dan dapat menerima serta mempelajari hal baru yang meningkatkan ilmu dan keterampillannya.
Clinical Characteristics of Eyes Showing a Discrete Margin of Different Retinal Reflectivity
Authors: Park Jin Heung , Kwon Soonil , Yang Hyeontak , Jeon Sohee 
Published: May 2021

BACKGROUND AND OBJECTIVE:
To evaluate the clinical characteristics of discrete margins of different retinal reflectivity (DMDRR) in ultra-widefield fundus photography.

PATIENTS AND METHODS:
This DMDRR comprises a discrete border, with differing patterns of reflectivity on either side. Spectral-domain optical coherence tomography was performed on both the macula and the margin. The incidences of epiretinal membrane (ERM) and peripheral retinal pathologies of patients with a DMDRR (n = 36) were compared with a control group (n = 41).

RESULTS:

An ellipsoid zone (EZ) defect at the DMDRR was detected in 34 eyes (92.2%), and vitreous traction was detected in 20 eyes (55.6%). A significantly higher proportion of eyes in the DMDRR group had an ERM, retinal hole, and cystic retinal tuft compared with the control group (
P
= .022,
P
= .010, and
P
< .001, respectively).

CONCLUSIONS:
The DMDRR indicates EZ disruption that may originate from vitreous traction. Meticulous observation for vitreoretinal interface diseases is mandatory for these patients.

[
Ophthalmic Surg Lasers Imaging Retina
. 2021;52:273–280.]
Quantitative Modeling of Ultra-Widefield Choroidal Indocyanine Green Angiography in Systemic Vascular Diseases
Authors: Kovacs Kyle D. , Young Benjamin , Adelman Ron A. 
Published: May 2021

BACKGROUND AND OBJECTIVE:
To evaluate quantitative measurements of choroidal vasculature as obtained via ultra-widefield indocyanine green angiography (ICGA) in patients with systemic vascular diseases.

PATIENTS AND METHODS:
Retrospective chart review and image analysis of 38 eyes from 21 patients with ICGA as part of routine retinal care. Images were binarized with lines drawn at specific antero-posterior landmarks. The vessel density and mean vessel caliber were measured along these lines and correlated with systemic vascular disease status.

RESULTS:

Unlike those with other systemic vascular diseases, patients with obstructive sleep apnea (OSA) were found to have increased choroidal vascular density and vessel caliber in all measurements of the peripheral choroid compared with those patients without (
P
< .05 for all measurements).

CONCLUSIONS:
Patients with OSA may have increased vascular density secondary to increased choroidal vessel caliber. Further work is needed to validate the modeling and confirm the association.

[
Ophthalmic Surg Lasers Imaging Retina
. 2021;52:281–287.]
ILF3 Is a Negative Transcriptional Regulator of Innate Immune Responses and Myeloid Dendritic Cell Maturation
Authors: Nazitto Rodolfo , Amon Lynn M. , Mast Fred D. , Aitchison John D. , Aderem Alan , Johnson Jarrod S. , Diercks Alan H. 
Published: May 2021

Abstract
APCs such as myeloid dendritic cells (DCs) are key sentinels of the innate immune system. In response to pathogen recognition and innate immune stimulation, DCs transition from an immature to a mature state that is characterized by widespread changes in host gene expression, which include the upregulation of cytokines, chemokines, and costimulatory factors to protect against infection. Several transcription factors are known to drive these gene expression changes, but the mechanisms that negatively regulate DC maturation are less well understood. In this study, we identify the transcription factor IL enhancer binding factor 3 (ILF3) as a negative regulator of innate immune responses and DC maturation. Depletion of ILF3 in primary human monocyte-derived DCs led to increased expression of maturation markers and potentiated innate responses during stimulation with viral mimetics or classic innate agonists. Conversely, overexpression of short or long ILF3 isoforms (NF90 and NF110) suppressed DC maturation and innate immune responses. Through mutagenesis experiments, we found that a nuclear localization sequence in ILF3, and not its dual dsRNA-binding domains, was required for this function. Mutation of the domain associated with zinc finger motif of ILF3’s NF110 isoform blocked its ability to suppress DC maturation. Moreover, RNA-sequencing analysis indicated that ILF3 regulates genes associated with cholesterol homeostasis in addition to genes associated with DC maturation. Together, our data establish ILF3 as a transcriptional regulator that restrains DC maturation and limits innate immune responses through a mechanism that may intersect with lipid metabolism.
Linking the SDGS with human rights: opportunities and challenges of promoting goal 17
Authors: Dulume Wekgari 
Published: May 2021

The 2030 Agenda for Sustainable Development is grounded in different international human rights instruments. Human rights (HR) principles and standards are strongly reflected in several of the Sustainable Development Goals (SDGs) and targets. Furthermore, SDG 17 emphasizes partnership as a key to achieving all of the SDGs. This article examines the SDGs-HR linkage in general, as well as specific HR principles that can be advanced by the achievement of SDG 17. Opportunities and challenges to promote Goal 17 of the SDGs that directly affect certain HRs are also examined. A review of relevant literature, 2030 summit documents, and outcomes of recent international conferences on the SDGs is undertaken in order to determine the progress made towards forging regional and global partnerships for the SDGs, as envisaged in Goal 17. This article finds that the absence of a political will and commitment, increased isolationist policy, narrow nationalism and poor rule linkage at national and international levels are some of the obstacles to the attainment of Goal 17. Yet, opportunities abound to promote the Goal. The article recommends a genuine commitment to implementing the SDGs by encouraging the South-South and North-South to prevent the SDGs from becoming a mere wishlist. Synergy between the government, individuals, civil society organizations (CSOs) and transnational corporations (TNCs) is equally very important. Keywords: Human rights, sustainable development goals, partnership for the goals.