bluuebunny/crossref_metadata_2025_split huggingface dataset:
ICAT Promotes Cervical Cancer Cell Proliferation, Invasion, Migration and Epithelial‑to‑mesenchymal Transition Through HPV16 E6, E7/ miR-23b-3p/ ICAT Axis
Authors: Hu Jing , Sun Zijiu , Wang Hui , Ren Wei , Fang Yuting , Hu Kai , Yu Huomei , Liao Deyu , Liu Shiyan , Zhou Lan , He Tongchuan , Zhang Yan 
Published: June 2021

Abstract
Background: Human papillomavirus (HPV) 16 plays a crucial role in cervical cancer (CC) development. Previous study reported that inhibitor of β-catenin and TCF (ICAT) is upregulated in CC and promotes cervical tumor progression. Herein, we aimed to investigate the underlying molecular mechanism that HPV16 regulates the expression of ICAT and promotes the CC development. Methods: The expressions of HPV 16 E6, E7 and ICAT were modulated by small interfering RNA and recombinant adenovirus, respectively. qRT-PCR was conducted to detect the mRNA expression of HPV 16 E6, E7, ICAT and miR-23b-3p in SiHa and CasKi cells. Bioinformatics analysis was utilized to predict the potential miRNAs that could bind to the ICAT 3′ untranslated region. Then, the dual luciferase reporter assay was used to confirm that. Cell proliferation ability was detected by CCK-8 assay. Wound healing and Transwell assays were used to observe migration and invasion abilities. Protein expressions were measured with western blot. Results: Results revealed that after knocking down of HPV16 E6, E7, the expression of ICAT decreased, but the expression of miR-23b-3p increased. Besides, miR-23b-3p negatively modulated ICAT expression in HPV16 positive CC cells. Dual luciferase assays confirmed that ICAT was a target gene of miR-23b-3p. Functional experiments showed that the overexpression of miR-23b-3p suppressed malignant behaviors of SiHa and CasKi cells, such as migration, invasion and EMT. Importantly, the overexpression of ICAT counteracted the suppressive effect of miR-23b-3p on HPV16 positive cervical cancer cell. Furthermore, after the knockdown of HPV16 E6 and E7, the inhibition of miR-23b-3p could increase the ICAT expression and rescue the siRNA HPV16 E6, E7-mediated suppressive impact on the aggressiveness of SiHa and CasKi cells.Conclusions: Our study demonstrates that HPV 16 E6, E7/miR-23b-3p/ ICAT axis plays an important role in HPV16 positive CC pathogenesis, which may serve as a promising therapy target for HPV 16-associated cervical cancer.
Functional and diffusion MRI reveal the neurophysiological basis of neonates’ noxious-stimulus evoked brain activity
Authors: Baxter Luke , Moultrie Fiona , Fitzgibbon Sean , Aspbury Marianne , Mansfield Roshni , Bastiani Matteo , Rogers Richard , Jbabdi Saad , Duff Eugene , Slater Rebeccah 
Published: June 2021

Abstract
Understanding the neurophysiology underlying neonatal responses to noxious stimulation is central to improving early life pain management. In this neonatal multimodal MRI study, we use resting-state and diffusion MRI to investigate inter-individual variability in noxious-stimulus evoked brain activity. We observe that cerebral haemodynamic responses to experimental noxious stimulation can be predicted from separately acquired resting-state brain activity (n=18). Applying this prediction model to independent Developing Human Connectome Project data (n=215), we identify negative associations between predicted noxious-stimulus evoked responses and white matter mean diffusivity. These associations are subsequently confirmed in the original noxious stimulation paradigm dataset, validating the prediction model. Here, we observe that noxious-stimulus evoked brain activity in healthy neonates is coupled to resting-state activity and white matter microstructure, that neural features can be used to predict responses to noxious stimulation, and that the dHCP dataset could be utilised for future exploratory research of early life pain system neurophysiology.
Traumatic Diaphragmatic Rupture in An Elderly Patient With Polytrauma
Authors: Le Son Thanh , Nguyen Hoe Trong , Ho Thanh Chi , Tiep Nguyen Van 
Published: June 2021

Abstract
Case presentation:
We report a case of an 80-year-old woman presented to our hospital 2 hours after a traffic accident with multiple injuries: blunt chest injury, rib fractures, pulmonary laceration, right pneumothorax, pleural effusion, right lower lobe atelectasis, right diaphragmatic rupture causing intrathoracic intestinal herniation, blunt abdominal trauma, grade III liver rupture, spine injury with L1, L2 transverse process fractures. An emergency surgery was performed to drain the fluid and air in the pleural cavity, evacuate the hernia organs, and repair the diaphragmatic injury. The postoperative source was uneventful and she was discharged after 20 days.
Conclusion
Early diagnosis of diaphragmatic rupture in polytrauma requires the combination of clinical examination, chest X-ray, and CT scanner. Favorable outcomes can be achieved with early surgical interventions.
Incremental Velocity Error As A New Treatment In Vestibular Rehabilitation (INVENT VPT Trial): Study Protocol For A Randomized Controlled Crossover Trial
Authors: Ervin Ann Margret , Schubert Michael , Migliaccio Americo , Perin Jamie , Coulibaly Hamadou , Millar Jennifer L. , Roberts Dale , Shelhamer Mark , Gold Daniel , Beauregard Stephanie , Pinto Robin , Brungart Douglas , Ward Bryan 
Published: June 2021

Abstract
Background: A clinical pattern of damage to the auditory, visual, and vestibular sensorimotor systems, known as multi-sensory impairment, affects a significant percent of the US population. Within the population of US military service members exposed to mild traumatic brain injury (mTBI), 15 – 44% will develop multi-sensory impairment following a mild traumatic brain injury. In the US civilian population, multi-sensory impairment related symptoms are also a common sequela of damage to the vestibular system and affect ~ 300-500/100,000 population. Vestibular rehabilitation is recognized as a critical component of the management of multi-sensory impairment. Unfortunately, the current clinical practice guidelines for the delivery of vestibular rehabilitation are not evidence-based and primarily rely on expert opinion. The focus of the INVENT VPT trial is gaze stability training, which represents the unique component of vestibular rehabilitation. The aim of the INVENT VPT trial is to assess the efficacy of a non-invasive, incremental vestibular adaptation training device for normalizing the response of the vestibulo-ocular reflex. Methods: The INVENT VPT trial is a multicenter randomized controlled crossover trial in which military service members with mTBI and civilian patients with vestibular hypofunction are randomized to begin traditional vestibular rehabilitation or incremental vestibular adaptation and then cross over to the alternate intervention after a prescribed washout period. Vestibulo-ocular reflex function and other functional outcomes are measured to identify the best means to improve delivery of vestibular rehabilitation. We incorporate ecologically valid outcome measures that address the common symptoms experienced in those with vestibular pathology and multi-sensory impairment. Discussion: The INVENT VPT Trial will directly impact the health care delivery of vestibular rehabilitation in patients suffering from multi-sensory impairment in three critical ways: 1) Compare optimized traditional methods of vestibular rehabilitation to a novel device that is hypothesized to improve vestibulo-ocular reflex performance; 2) Isolate the ideal dosing of vestibular rehabilitation considering patient burden and compliance rates; 3) Examine whether recovery of the vestibulo-ocular reflex can be predicted in participants with vestibular symptoms. Trial registration: ClinicalTrials.gov, NCT03846830. Registered 20 February 2019,https://clinicaltrials.gov/ct2/show/NCT03846830
Glycine-induced NMDA receptor internalization provides neuroprotection and preserves vasculature following ischemic stroke
Authors: Bergeron Richard , Cappelli Julia , Khacho Pamela , Wang Boyang , Sokolovski Alexandra , Bakkar Wafae , Raymond Sophie , Ahlskog Nina , Pitney Julian , Wu Junzheng , Chudalayandi Prakash , Wong Adrian 
Published: June 2021

Abstract
Ischemic stroke is the second leading cause of death worldwide. Compelling evidence demonstrates that following an ischemic event, neuronal death is triggered by uncontrolled glutamate release leading to overactivation of glutamate sensitive N-methyl-D-aspartate receptor (NMDAR). For gating, NMDARs require not only the binding of glutamate, but also the binding of glycine or a glycine-like compound as a co-agonist. Glycine fulfills several roles in biology including protein synthesis, inhibitory transmission via glycine receptor activation and excitatory transmission through NMDARs. Low glycine doses enhance NMDAR function while high doses trigger glycine-induced NMDAR internalization (GINI) in vitro. The physiological relevance of GINI has been questioned given that the high-affinity glycine transporter type 1 (GlyT1), located on astrocytes and neurons, maintains synaptic glycine concentrations far below the level that would saturate the glycine binding site (GBS). Here, we report that following an ischemic event, in vivo, GINI also occurs and provides neuroprotection. Mice pre-treated with a GlyT1 antagonist (GlyT1-A), which increases glycine synaptic levels, exhibited smaller stroke volume, reduced cell death, and minimized behavioural deficits following stroke induction by either photothrombosis or endothelin-1. Therefore, increasing glycine levels in the synaptic cleft will enhance GBS occupancy and will reach the set point to trigger GINI. However, we report that GINI is triggered during ischemic stroke, in vivo, only in the presence of GlyT1-As. Moreover, we show evidence that in ischemic conditions, GlyT1-As preserve the vasculature in the peri-infarct area. Therefore, the clinical efficacy of GlyT1-As should be tested for the treatment of ischemic stroke.
The epidemiology and outcome of burn injuries in Iran: A ten-year systematic review and meta-analysis
Authors: Mobayen Mohammadreza , Ghaffari Mohammad Ebrahim , Shahriari Fatemeh , Gholamrezaie Sepideh , Dogahe Zahra Haghani , Chakari-Khiavi Aref 
Published: June 2021

Abstract
Background: Burns, has always been one of the most catastrophic injuries. More than 3000000 people lose their lives every year due to burn and its results. Burn injury is the seventh major cause of disease burden of injuries in Iran. The evidence clearly shows that burns are preventable. It is necessary to update existing information and a more detailed assessment of burn aspects. Material-Method: Web of Science, PubMed, Scopus, and Embase databases were searched for published studies from Jan. 2010 to Dec. 2020, with the MeSH term ‘Burns’ and keywords such as burn, burn injury, burn injuries, Iran, epidemiology, and epidemio* combined by OR; AND, for published retrospective studies that focus on the epidemiology of burns in Iran. Results: Our systematic search identified seventeen studies containing epidemiological characteristics of burn injuries in Iran with a total number of 61662 patients. Results revealed that: The highest and lowest percentage of the burn was related to the age group of 20-29 and 0-9 years and 50-59 years; men were burned more than women. The urban residents and the person under the diploma have experienced more burns; married and single people were equally burned; burns caused by flame occurred more than the other cause of burn; male and female were burned almost equally by flame; Contact and electrical burns in men were more than women; most burn accidents occurred at home and winter; 14/23 % patient were expired, and men were discharged more than women but the death rate was higher in women.Conclusion: We conclude that burn injuries are common indoors, caused by flames, and in young patients with a low educational state, and the overall mortality of female burn victims is more remarkable than male burn victims. On the other hand, the mean TBSA of burn patients in Iran was 31%, and the mean rate of mortality was 14%, both of which are greater than the statistics reported by WHO. This review shows that further research is required to summarize the situation and the effectiveness of any preventive interventions.
Understanding hesitancy towards vaccination against SARS-COV2 among Health professionals in Tunisia
Authors: Zammit Nawel , ElGuedr Amani , Brahem Aïcha , Ghammam Rim , Fredj Sihem Ben , Ayouni Imen , Sr Chaima , Chouchene Asma , Kalboussi Houda , ElMaalel Olfa , Chatti Souhaeil , Maatoug Jihene , Ghannem Hassen , Mrizak Néjib 
Published: June 2021

Abstract
Background: Since the end of 2019, the world was seriously upset by the emergence of the “COVID-19 disease. Vaccines that were authorized for emergency use in a wide range of countries brought a glimmer of hope. However, sufficient vaccination coverage is conditioned by the people’s acceptance of these vaccines especially by health professionals. Indeed, they represent the leaders of the current war against COVID-19. Several studies focused on this issue in developed countries. However, few were reported from developing ones including Tunisia.Objective: The current study aimed to estimate the prevalence and the predictors of hesitancy towards the vaccination against the SARS-COV2 among the Tunisian health professionals.Methods: A cross-sectional study was led online between the 7th and the 21th of January 2021 among Tunisian health professionals. A number of at least 460 participants was required. Snowball sampling method served to recruit participants. Data were collected using a pre-established and pre-tested questionnaire recorded in a free Google form. The link of the questionnaire was disseminated online to be self-administered anonymously to the participants. The generated online Google Sheet was uploaded and exported to SPSS software. Responses of non eligible participants were deleted before analysis.Results: Of the 546 responses, 493 were retained. The mean age was 37.4 (±9.5) years. Females represented 70.2% of participants. Social media represented the most frequently used source of information about SARS-COV2 (reported by 66.9% of participants). Prevalence of hesitancy towards COVID-19 vaccination was 51.9% (95% CI: 47.5-56.3)). Fear of eventual harmful components in the upcoming vaccines, female gender and having its professional activity far from the capital predicted more hesitancy among participants. However, a history of previous infection by SARS-COV2 and the use of the official national site for information about COVID19 predicted less hesitancy towards COVID-19 vaccination.Conclusions: An effective national information campaign is required to reduce hesitancy towards COVID-19 vaccination among Tunisian Health professionals. More international solidarity would increase vaccine availability in developing countries such Tunisia and ensure therefore faster resolution of the current pandemic.
Using k-mer Embeddings Learned from a Skip-Gram Based Neural Network as Effective Feature Representation for Building a Cross-Species Prediction Model to Identify DNA N6-Methyladenine Sites in Plant Genomes
Authors: Trung Duong Nguyen Trinh , Trinh Van-Ngu , Le Nguyen Quoc Khanh , Ou Yu-Yen 
Published: June 2021

Abstract
Identification of DNA N6-methyladenine sites has been a very active topic of computational biology due to the unavailability of suitable methods to identify them accurately, especially in plants. Substantial results were obtained with a great effort put in extracting, heuristic searching, or fusing a diverse types of features, not to mention a feature selection step. We considered DNA, the human life book, as a book corpus for training DNA language models. K-mer embeddings then were generated from Skipgram neural networks and input into several ensemble tree-based algorithms. We trained the prediction model on Rosaceae genome dataset and performed a comprehensive test on 3 plant genome datasets. Our proposed method shows promising performance with AUC performance approaching an ideal value on Rosaceae dataset (0.99), a high score on Rice dataset (0.95) and improved performance on Rice dataset while enjoying an elegant, yet efficient feature extraction process.
The Food Additive Xanthan Gum Drives Adaptation of the Human Gut Microbiota
Authors: Martens Eric , Ostrowski Matthew , Rosa Sabina La , Kunath Benoit , Robertson Andrew , Pereira Gabriel , Hagan Live , Varghese Neha , Qiu Ling , Yao Tianming , Flint Gabrielle , Li James , McDonald Sean , Martin Duna , Pudlo Nicholas , Schnizlein Matthew , Brumer Harry , Schmidt Thomas , Terrapon Nicolas , Lombard Vincent , Henrissat Bernard , Hamaker Bruce , Eloe-Fadrosh Emiley , Tripathi Ashootosh , Young Vincent 
Published: June 2021

Abstract
The diets of industrialized countries reflect the increasing use of processed foods, often with the introduction of novel food additives. Xanthan gum is a complex polysaccharide with unique rheological properties that have established its use as a widespread stabilizer and thickening agent1. However, little is known about its direct interaction with the gut microbiota, which plays a central role in digestion of other, chemically-distinct dietary fiber polysaccharides. Here, we show that the ability to digest xanthan gum is surprisingly common in industrialized human gut microbiomes and appears to be contingent on the activity of a single bacterium that is a member of an uncultured bacterial genus in the family Ruminococcaceae. We used a combination of enrichment culture, multi-omics, and recombinant enzyme studies to identify and characterize a complete pathway in this uncultured bacterium for the degradation of xanthan gum. Our data reveal that this keystone degrader cleaves the xanthan gum backbone with a novel glycoside hydrolase family 5 (GH5) enzyme before processing the released oligosaccharides using additional enzymes. Surprisingly, some individuals harbor a Bacteroides species that is capable of consuming oligosaccharide products generated by the keystone Ruminococcaceae or a purified form of the GH5 enzyme. This Bacteroides symbiont is equipped with its own distinct enzymatic pathway to cross-feed on xanthan gum breakdown products, which still harbor the native linkage complexity in xanthan gum, but it cannot directly degrade the high molecular weight polymer. Thus, the introduction of a common food additive into the human diet in the past 50 years has promoted the establishment of a food chain involving at least two members of different phyla of gut bacteria.
Social Risk Factors Associated With Daily Tobacco Smoking and Binge Drinking Among Zambians: Evidence From the 2017 STEPS Survey
Authors: Silumbwe Adam , Sabastian Miguel San , Michelo Charles , Zulu Joseph Mumba , Johansson Klara 
Published: June 2021

Abstract
Background: The burden of disease attributable to tobacco smoking and harmful alcohol consumption poses a major threat to sustainable development in most low- and middle-income countries. However, evidence on tobacco use and harmful alcohol consumption to inform context-specific interventions addressing these harmful social behaviours is limited in the African context. This study aimed to determine the social risk factors associated with daily tobacco smoking and binge drinking in Zambia.Methods: The study stems from nationwide population-based representative survey data collected using the World Health Organization’s STEPwise approach for non-communicable disease risk factor surveillance in 2017 among 18–69-year-old Zambians. The main outcomes were daily tobacco smoking and binge drinking, and the demographic and socioeconomic variables included sex, marital status, age, residence, level of education and occupation. Prevalence ratios were calculated using log-binomial regression analysis.Results: Overall, 4302 individuals (weighted percentage 49.0% men and 51.0% women) participated in the survey. The prevalence of daily tobacco smoking was 9.04%, and 11.6% of participants engaged in binge drinking, both of which were higher among men than women (17.1% vs. 1.28% and 18.6% vs. 5.29%, respectively). The risk of daily tobacco smoking was significantly higher in men (PR 14.3; 95% CI [9.74, 21.0]) than women, in the >45-year-old group (PR 1.44; 95% CI [1.03, 1.99]) compared to the 18–29-year-old group, and in those with no education (PR 2.70; 95% CI [1.79, 2.99]) or primary education (PR 1.86; 95% CI [1.22, 2.83]) compared to those with higher secondary or tertiary education. The risk of binge drinking was 3.67 times higher (95% CI [2.83, 4.76]) in men than in women. Significantly lower levels of binge drinking were observed among rural residents (PR 0.59; 95% CI [0.46, 0.77]) compared to urban residents and students/homemakers (PR 0.58; 95% CI [0.35, 0.94]) compared to the employed.Conclusion: This study shows huge differences between men and women regarding tobacco smoking and binge drinking in Zambia. A high occurrence of tobacco smoking was observed among men, older members of society and those with lower levels of education, while binge drinking was more common in men and in those living in urban areas. There is a need to reshape and refine preventive and control interventions for tobacco smoking and binge drinking to target the most at-risk groups in the country.
Comparison Between Percutaneous Kyphoplasty And Percutaneous Vertebroplasty In Terms of The Efficacy For Osteoporotic Vertebral Compression Fractures: A Meta-Analysis
Authors: Zhu Hai-Tao , Ding De-Gang , Wang Shui , Zhu Yu-Long 
Published: June 2021

Abstract
Background & Aim: Osteoporotic vertebral compression fractures (OVCFs) are acknowledged as common occurred fractures among humans, especially for the elderly population. The minimally invasive percutaneous methods such as kyphoplasty as well as vertebroplasty have been valid and effective tools in terms of reducing clinical problems, which are associated with more beneficial effects as compared to traditional methods such as open surgery or conservative treatment. Hence, we conducted the current meta-analysis in order to gather updated evidence available for the systematical assessment of the clinical and radiographic outcomes of VP in comparison of KP.Methods: Publications on comparison kyphoplasty versus vertebroplasty in treatment of OVCFs were collected. After rigorous and thorough review of quality, we extracted the data on the basis of eligible trials, which analyzed the summary hazard ratios (HRs) of the endpoints of interested.Results: Totally, our inclusion criteria involved five studies. 561subjects involving 334 patients received VP and 227 patients received KP were included, of which patients receiving KP or VP failed to show any significant differences in the visual analog scale (VAS) scores (MD =-0.02, 95% CI -0.40 – 0.35; P = 0.91),and the risk of cement leakage (OR =1.60, 95% CI 0.82 – 3.12; P = 0.17). Nevertheless, the Oswestry Disability Index (ODI) scores (MD =-1.56, 95% CI -2.39 – -0.73; P = 0.0002), the injected cement volume (MD =-0.40, 95% CI -0.77 – -0.04; P = 0.03) and the operation time (MD =-9.67, 95% CI -11.55 – -7.80; P ＜0.00001) in VP group were linked to markedly lower trend in comparison of KP group with significant statistical difference.Conclusion: The present meta-analysis controls the acceptable level of the efficacy across the involved trials. The VP had ODI scores, the injected cement volume and the operation time exerted several advantages in this meta-analysis. Yet, VP failed to show benefits in terms of the VAS scores and cement leakage in comparison of KP therapy. Given the combined results of our study, the optimal treatment for patient harboring OVCFs should be determined by further high-quality and multi-center RCTs along with longer follow-ups as well as larger sample size.