bluuebunny/crossref_metadata_2025_split huggingface dataset:
Osteogenic Differentiation of Pre-Conditioned Bone Marrow Mesenchymal Stem Cells with Nisin on the Modified Poly-L-Lactic-Acid Nanofibers
Authors: Sadraei Fariba , Ghollasi Marzieh , Khakpai Fatemeh , Halabian Raheleh 
Published: June 2021

Abstract
Background: Human bone marrow-derived mesenchymal stem (MSCs) cells are undifferentiated cells with the self-renewing ability and multi-lineage differentiation beneficial for regenerative medicine. Nano scaffolds are novel materials employed in bone repair and regeneration. Nisin is a prebiotic that can increase stem cells’ life span and proliferation. This study attempted to provide a proper strategy for bone marrow mesenchymal stem cells differentiation into the Osteocytes on a Poly‐L‐lactic‐acid scaffold (PLLA) after pretreating with probiotic Nisin. Methods: MSC osteogenic differentiation was evaluated by measuring Calcium, Alkaline phosphatase, and quantitative tests such as Real-Time PCR, Acridine Orange, Alizarin Red, Von Kossa, and others. Results: The result of the MTT test showed that the optimal dose of Nisin probiotic for the MSCs’ preconditioning was 200 IU/mL on the 1st, 3rd, and 5th days of culture. Real-time PCR data indicated that the expression rate of ALP, Osteonectin, Osteocalcin, and Collagen I have increased in the presence of Nisin, while the RUNX-2 gene expression has decreased. Furthermore, the results of Alizarin Red and Von Kossa tests, as well as Scanning electron microscopy (SEM), revealed that the cell proliferation in the preconditioned samples with Nisin increased significantly. Conclusions: The study concluded that the cell proliferation and differentiation increased in samples pretreated with Nisin on the PLLA Nano scaffolds.
Reconstruction and Repairment With Mini-plate and Bone Graft for HIV Positive Patients of Giant Cell Tumor of Long Bone: Retrospective Analysis of a Single-center Experience
Authors: Xu Biao , Ma Rui , Zhang Qiang , Zhao Chang-song , Zhang Wen-sheng , Wang Jie 
Published: June 2021

Abstract
Background: To evaluate the effect of reconstruction and repairment with mini-plate and bone graft for HIV positive patients of giant cell tumor of long bone.Methods: This research retrospectively analyzed 12 HIV positive patients with giant cell tumor of long bone, 11 male and 1 female, with a age range 16 to 68 years old (43.5 years old on average) were included. There were 5 cases of proximal tibia，3 cases of distal femur, 2 case of distal tibia, and 2 case of talus. From June 2012 to August 2020, curettage by ultrasonic scalpel were performed in all patients, combined with min- plate and bone graft treatment. All patients were followed up for 18-60 months. Limb function was evaluated by MSTS93 scoring system, and postoperative recurrence and distant metastasis, complications, MSTS93 score and fracture prognosis were observed.Results: No local recurrence and pulmonary metastases was observed. After surgery, all the 12 patients showed good bone morphologic repair and reconstruction, good bone healing , good joint function, and no pathological fracture around the lesion. One case of giant cell tumor of proximal tibia showed mild articular surface collapse, and mild valgus deformity of knee joint, but good joint function. The MSTS93 score of the patients 6 months after the operation was 24-27 points (24.5±1.08), with a significant difference (P < 0.05).Conclusion: Reconstruction and repairment with mini-plate and bone graft for HIV positive patients of giant cell tumor of long bone has achieved satisfactory results. The mini- plate takes up little space and is flexible for reconstruction and fixation, significantly reducing complications such as surgical site infection, preserving joint function and avoiding amputation. It is a safe and effective treatment method.
New Heterometallic Compound as Luminescent Sensor for Detection of CS2 and Treatment Effect on Childhood Diabetes
Authors: Sun Wen-Hui , Ye Bing 
Published: June 2021

Abstract
The hydrothermal reaction of Cd(NO3)2·4H2O, KNO3 with the dicarboxylate ligand of 2-aminoterephthalic acid (H2L) yields a new heterometallic coordination polymer with the formula of [CdK2(L)2(H2O)4]n (1). Compound 1 emits intense luminescence at ambient temperature and shows high selectivity and sensitivity for the detection of CS2. Serial biological experiments were conducted to evaluate the activity of the new compound on children diabetes. First of all, after the compound treatment, the blood glucose meter was used to measure the levels of body's blood sugar. In addition to this, the relative expression levels of the insulin receptor on the liver cells were determined with real time RT-PCR.
m(6)A mRNA Methylation Regulates Ferroptosis in HPSCC by Targeting NFE2L2/NRF2
Authors: Ye Jing , Chen Xiaozhen , Liao Bing , Jiang Xiaohua , Dong Zhihuai , Hu Sunhong , Liu Yuehui , Xiao Mang 
Published: June 2021

Abstract
Background

Emerging as the most abundant posttranscriptional internal mRNA modification in eukaryotes, N6-methyladenosine (m6A) modification has gathered tremendous scientific interest in recent years. However, no study addresses the role of m6A modification in ferroptosis. Here, we showed that m6A modifications are decreased in RSL3-induced ferroptosis in hypopharyngeal squamous cell carcinoma (HPSCC). We found that AlkB homolog 5 (ALKBH5), one of the m6A demethylases, is the primary factor involved in aberrant m6A modification.
Methods

Bioinformatics analysis, sample analysis, cell biological analyses and transcriptome sequencing were performed to evaluate the correlation between m6A modification and ferroptosis as well as molecular mechanism of ALKBH5 function. Transcriptome-wide m6A-seq and RIP-seq data and following m6A dot blot, MeRIP-qPCR, RIP-qPCR and dual luciferase reporter assays were mapped to screen and validate the candidate targets of ALKBH5.
Results

ALKBH5-knockdown impaired ferroptotic cell death in HPSCC. However, overexpression of ALKBH5 has an opposite effect, suggesting that ALKBH5 is a positive regulator of ferroptosis. Mechanistically, ALKBH5-mediated m6A demethylation led to a post-transcriptional inhibition of NFE2L2/NRF2, the central player in the regulation of antioxidant molecules in cells, at two m6A residues in the 3ʹ-UTR. Therefore, knocking down ALKBH5 subsequently increases the expression levels of NFE2L2/NRF2 and increased cell resistance to ferroptosis. In addition, m6A-mediated NFE2L2/NRF2 stabilization relied on the m6A reader IGF2BP2.
Conclusion

ALKBH5 functions as a tumor suppresser through ferroptosis in HPSCC. ALKBH5 destabilizes NFE2L2/NRF2 expression in HPSCC through an m6A-IGF2BP2-dependent mechanism. Together, our work uncovers a critical link between ALKBH5-NFE2L2/NRF2 and ferroptosis, providing insight into the functional importance of the reversible mRNA m6A methylation and its modulators in HPSCC.
Mapping Childcare Support for Patients at North American Hospitals and Cancer Centres: An Environmental Scan.
Authors: Preston Katherine , MacDonald Mackenzie , Giuliani Meredith , Leung Bonnie , Simmons Christine , Melosky Barbara , Ingledew Paris-Ann 
Published: June 2021

Abstract
Purpose
Approximately one quarter of cancer patients are parents to young children. One unique challenge faced by this patient group is the difficulty of accessing childcare support during medical appointments. Hospital-based childcare options could represent a solution to this problem, but to this point, no comprehensive scans have described existing on-site childcare centres. The purpose of this study is to identify and characterize on-site childcare services available to patients at North American hospitals. This information could inform the development of similar programs for cancer patients.
Methods
Using publicly available information, an environmental scan of the grey literature was conducted to investigate Canadian and American hospitals for the presence of childcare services. A standardized data collection tool was used to extract centre characteristics.
Results
Twenty-six childcare centres were identified across 161 hospitals. The majority of the centres were associated with pediatric hospitals (77%) and were located in the United States (69%). Only a single childcare centre was associated with a cancer hospital. All centres accommodated children between the ages of 3 and 8. Most centres were open for over 30 hours per week (77%) and were free of charge to users (89%). Other characteristics, including capacity and staffing, varied widely.
Conclusions
These results represent an inventory of patient-accessible, on-site childcare services currently available at North American hospitals. Cancer patients who are also parents may especially need this kind of support, as they are at high risk for financial toxicity.
Characteristics and Clinical Outcomes of Patients With Nonsmoking Small Cell Lung Cancer in Korea
Authors: Kang Hye Seon , Lim Jung Uk , Yeo Chang Dong , Park Chan Kwon , Lee Sang Haak , Kim Seung Joon 
Published: June 2021

Abstract
Background: The aim of this study was to investigate the characteristics and clinical outcomes of patients with nonsmoking small cell lung cancer (SCLC) using a nationwide registry in Korea.Methods: The Korean Association for Lung Cancer developed a registry in cooperation with the Korean Central Cancer Registry (KCCR) and surveyed approximately 10% of recorded lung cancer cases. Results: From 2014 to 2016, the KCCR registered 1,043 patients newly diagnosed with SCLC among a total of 8,110 lung cancer patients. In subgroup analysis, the overall survival (OS) was significantly shorter in the nonsmoking subgroup than the ever-smoking subgroup of SCLC patients with extensive disease (6.99 vs. 9.68 months; P = 0.004). Among SCLC patients with limited disease, OS was also shorter in the nonsmoking subgroup, without statistical significance (19.4 vs. 23.5 months; P = 0.179). In a multivariate analysis using a Cox regression model, never smoking was not associated with shorter OS, but older age, extensive stage, poor performance status (Eastern Cooperative Oncology Group grade ≥2), male sex, no prophylactic cranial irradiation, and no active treatment (chemotherapy and/or radiotherapy) were associated with poor prognosis.Conclusion: This evaluation of an unbiased nationwide survey dataset revealed that a significant proportion of Korean SCLC patients were never-smokers. No history of smoking appeared to be a significant prognostic factor according to the univariate analysis but was confirmed to be statistically insignificant through a multivariate analysis of the total population. Reasons for a poor prognosis may include the possibility that a high rate of the elderly population is composed of nonsmokers who did not receive active treatment.
Effect of Mindfulness Sessions on Chronic Fatigue Syndrome and Job Satisfaction of Nurses: A Randomized Clinical Trial
Authors: Bonakdari Nasrin , Mohammadi Nadereh , Yazdi Mohammad Reza Taghvizadeh , Norouzadeh Reza , Abbasinia Mohammad , Aghaie Bahman 
Published: June 2021

Abstract
Background: Nursing is considered a stressful profession, so nurses are at higher risk of physical and mental illness. Mindfulness is an important concept for nursing with practical implications for nurse well-being, development, and quality nursing care sustainability.Objectives: This study identified mindfulness sessions' effect on nurses' chronic fatigue syndrome(CFS) and job satisfaction )JS.(Methods: A randomized clinical trial design was conducted on 80 nurses to intervention and control groups. The intervention in the intervention group was eight mindfulness sessions training, and the control group had no intervention. Nurses' CFS and JS were assessed using the Minnesota Satisfaction Questionnaire and The Multidimensional Fatigue Inventory.Results: After implementing a mindfulness-based stress reduction program, the intervention group nurses reported lower CFS and higher JS than the control group(p<0.005). Conclusion: The findings of this study indicate the effectiveness of mindfulness programs on improving nurses' chronic fatigue and JS. Since CFS and job dissatisfaction are two disturbing elements for nurses' optimal performance in professional and personal life, this study can recommend the implementation of mindfulness exercises as a routine, operational and uncomplicated program in nurses.
Yield Improvement of Gracilaria tentuistipitata by Optimizing Different aspects in Coast of Cox’s Bazar, Bangladesh
Authors: Bokhtair S. M. , Ali M. A. , Chowdhury M . A. Z. , Ahmed K. U. , Hassan M. K. , Ahmed M. , Bhuiyan M. S. , Mashuk O. F. , Rahman M M , Rafiquzzaman S. M. 
Published: June 2021

Abstract
This research was designed to find out the effect of different factors such as influence of lunar cycle, harvesting interval, rope type and seeding gap on the production of G. tenuistipitata in coast of Cox’s Bazar. Duration of these experiments were sixty days and all the parameters were recorded fortnightly. Monitoring of water quality parameters indicated that salinity, temperature, transparency, pH and DO were suitable for seaweed cultivation. In determining lunar cycle effect, results envisaged that fresh yield was 14.43% increased when seeding and harvesting time was selected considering the moon cycle. Regarding the selection of harvesting interval, it was found that T3 (30 days interval) was the best to harvest the seaweed whereas T4 (40 days interval) showed decreasing trend in production. Our study also found that semi floating single line showed better yield performance compared to semi floating double line system. In case of influence on seeding gap, it has been found that 20 cm gap between two seed showed the highest yields followed by 10 cm, 30 cm and 40 cm, respectively. Overall, it can be concluded that yield of G. tenuistipitata in coast of Cox’s Bazar could be improved considering those factors.
Efficacy of Dual PI3K/mTOR Inhibitor Combined With Autophagy Suppression Against Laryngeal Squamous Cell Carcinoma
Authors: Huang Hui-ying , Li Ke-Nan , Lau Hui-Ching , Hsueh Chi-Yao , Cong Ning , Zhang Ming 
Published: June 2021

Abstract
Objective: We explored the antitumor effect of dual PI3K/mTOR inhibitor combined with autophagy suppression on laryngeal squamous cell carcinoma (LSCC) and its underlying mechanism.Methods: Hep-2 and AMC-HN-8 cell lines were treated with the Akt inhibitor LY294002, mTOR inhibitor rapamycin, and dual inhibitor NVP-BEZ235 separately. The biological characteristics of in vitro proliferation, cell cycle, apoptosis, migration, invasion, and autophagy were analyzed, and the expression levels of PI3K/Akt/mTOR pathway-related proteins were also measured. The in vivo effects of NVP-BEZ235 combined with inhibition of autophagy using pharmacological inhibitor was further assessed.Results: Compared with Akt or mTOR inhibitor, NVP-BEZ235 had the most significant biological effects on LSCC cells. When combined with various autophagy inhibitors, along with siRNA against ATG7, NVP-BEZ235 showed a synergic antitumor effect in LSCC through increasing cell apoptosis and death both in vitro and vivo.Conclusion: NVP-BEZ235 exerted stronger antitumor effects on LSCC, especially when combined with the autophagy inhibitor, providing convincing experimental data for new molecular targeted therapy for LSCC.
Comprehensive Analysis of Expression and Prognostic Value for JMJD5 and PKM2 in Stomach Adenocarcinoma
Authors: Qi Ling , Li Li-Sha , Zhan Chao , Jiang Ming-Xia , Song Dong-Feng , Wu Yi-Ming , Gan Jun-Qing , Jin Ke-Xin , Huang Mei , Li yanjing , Du Xiao-Xue , Song Cheng-Xin 
Published: June 2021

Abstract
Background: Jumonji C-domain-containing (JMJD) family, a group of genes that regulate epigenetics, is involved in tumor development in several types of cancer. JMJD5 is a member of the JMJD family, and its clinical impact on stomach adenocarcinoma (STAD) remains unclear. Pyruvate kinase M2 (PKM2) promotes metabolism, tumor proliferation, and metastasis in various cancer types. However, the relationship between JMJD5 and PKM2 in STAD is yet to be established. In this study, we investigated the expressions and relationship of JMJD5 and PKM2 in patients with STAD. Furthermore, we evaluated the clinical significance between their expression and prognosis. In addition, we explored the transcriptional and survival effects of other 7 members of the JMJD family including JMJD1B, JMJD1C, JMJD2D, JMJD4, JARID2, HSPBAP1, TYW5 in patients with STAD.Methods: The expression of JMJD5 and PKM2 in STAD was examined using western blot, quantitative real-time polymerase chain reaction (RT-qPCR), and immunohistochemical staining. Statistical analyses were performed using the SPSS 22.0 statistical software program. The roles of JMJD1B, JMJD1C, JMJD2D, JMJD4, JARID2, HSPBAP1, TYW5 in STAD were examined using UALCAN, GEPIA, Kaplan–Meier Plotter,the Human Protein Atlas, STRING, the cBiopotal, Metascape databases.Results: We discovered that the rates of low expression of JMJD5 and high expression of PKM2 in the tumor cells of STAD were 64.52.% and 62.37%, respectively. Moreover, there was a close connection between the expressions of JMJD5 and PKM2. We uncovered that the low expression of JMJD5 was related to poor differentiation (P = 0.002) and large tumor size (P = 0.044). The survival rate was low in patients with low expression of JMJD5 and high expression of PKM2. In addition, we found that JMJD1B, JMJD1C, JMJD2D, JMJD4, JARID2, HSPBAP1, TYW5 were high in the STAD tissues. Besides, gene expression levels were correlated with tumor stage and grade. Survival analysis demonstrated that high expressions of these genes, except JMJD1B, were associated with low survival rates. Moreover, a high mutation rate of these genes (82.22%) was observed in STAD patients.Conclusions: These findings implied that JMJD1B, JMJD1C, JMJD2D, JMJD4, JMJD5, JARID2, HSPBAP1, TYW5, and PKM2 could serve as potential therapeutic targets in patients with STAD and as novel biomarkers for the disease.
Effect of Hybrid Texture on Cutting Performance of WC/Co-based Coated Tools Turning Aluminum Alloy
Authors: Fu Yonghong , Zhou Yun , Yang Zetan , Yang Jie 
Published: June 2021

Abstract
When cutting aluminum alloy with WC/Co coated tools, severe adhesion and wear exist on the tool-chip contact interface, which are the major factors leading to failure of cutting tools. To address this problem and extend service life of cutting tools, this study introduced surface textures into coated tools, trying to integrate the anti-friction properties of micro-textures with the wear resistance properties of coatings to improve tribological characteristics of tools surface. Firstly, hybrid texture consisting of micro-scale pits and micro-scale grooves were fabricated on the rake surface close to the main cutting edge of carbide tools by picosecond laser. Subsequently, the textured tools were deposited with hard-coatings CrAlN via physical vapor deposition (PVD) technology and the hybrid textured CrAlN coated tool (MPG-T) was obtained eventually. Other texture combinations included micro-scale pits and micro-scale grooves distributed in the tool-chip contact zone. Wet cutting experiments were carried out on these prepared tools. Results showed that compared with micro-scale pitted coated tool (MP-T), micro-scale grooved (MG-T) and conventional coated tool (CCT), MPG-T tool performed better in cutting forces, friction coefficient, tool adhesion and wear on the rake face and the flank face, chip morphology. Moreover, the corresponding synergistic mechanisms of hybrid texture and coatings were proposed. It’s suggested that applying the results to actual industries can enhance the cutting performance of coated tools in machining of aluminum alloy.
Parathyroidectomy Protects Against Pathologic Fractures After Kidney Transplantation: Results From a Retrospective Single-center Trial
Authors: Jehn Ulrich , Kortenhorn Anja , Schütte-Nütgen Katharina , Thölking Gerold , Westphal Florian , Strauss Markus , Wennmann Dirk , Pavenstädt Hermann , Suwelack Barbara , Görlich Dennis , Reuter Stefan 
Published: June 2021

Abstract
Kidney transplant (KTx) recipients are a high-risk population for pathologic fractures. We herein aim to identify modifiable factors associated with pathological fractures and bone disease in KTx recipients. We conducted a retrospective study involving 711 adult patients (4608 patient-years) who were transplanted at our center between January 2007 and June 2015. Clinical data was extracted from patients’ electronic medical records. Different laboratory and clinical parameters for mineral bone disease (MBD) and osteoporosis including medication were evaluated. We chose fracture events unrelated to malignancies or adequate trauma as the primary endpoint. Pathologic fractures occurred in 47 (6.6%) patients (median 36.7 months, IQR 45.9) after KTx, fracture incidence of 10 per 1000 person-years). Prior to KTx, (subtotal) parathyroidectomy (PTX) was performed in 116 patients (16.3%) (median time 4.2 years before KTx, IQR 5.0). Of the patients with fracture (n= 47), only one (2.2%) patient was parathyroidectomized. After adjusting for known fracture risk factors for MBD and osteoporosis, PTX remained a protective factor against fractures (HR 0.134, CI 0.018 – 0.991, p=0.049). We observe a reduced risk for pathological fractures in parathyroidectomized KTx patients independent from elevated parathyroid hormone at the time of KTx or afterwards.
A Correlation Study between Clinical Efficacy of Weifuchun on Precancerous Lesions of Gastric Cancer and Intestinal flora
Authors: Bian Yanqin , Chen Xi , Cao Hongyan , Xie Dong , Zhu Meiping , Yuan Nong , Lu Lu , Lu Bingjie , Wu Chao , Azami Nisma Lena Bahaji , Wang Zheng , Wang Huijun , Zhang Yeqing , Li Kun , Sun Mingyu , Ye Guan 
Published: June 2021

Abstract
BackgroudWeifuchun(WFC), a Chinese herbal prescription consisting of Red Ginseng, Isodon amethystoides and Fructus Aurantii, is commonly used in China to treat a variety of chronic stomach disorders. The aim of the paper is to determine the effect of WFC on intestinal microbiota changes in precancerous lesions of gastric cancer (PLGC) patients. MethodsPLGC patients of H.pylori negative were randomly divided into two groups received either WFC tablets at a dose of 1.44g 3 times a day or vitacoenzyme tablets at a dose of 0.8g 3 times a day. All patients were treated for 6 months, consecutively. Gastroscopy and histopathology were used to assess the histopathological changes in the gastric tissues before and after treatment. 16S rRNA gene sequencing was carried out to assess the effects WFC on intestinal microbiota changes in PLGC patients. Receiver Operating Characteristics(ROC) analysis was used to assess the sensitivity and specificity of different intestinal microbiota in distinguishing between PLGC patients and healthy control. ResultsGastroscopy and histopathological results indicated that WFC could improve the pathological condition of PLGC patients, especially in the atrophy or intestinal metaplasia. The results of 16S rRNA gene sequencing indicated that WFC could regulate the microbial diversity, microbial composition, and abundance of the intestinal microbiota of PLGC patients. Following WFC treatment, the relative abundance of Parabacteroides was decreased in WFC group when compared with the control group. ROC analysis found that the Parabacteroides could effectively distinguish PLGC patients from healthy individuals with sensitivity of 0.79 and specificity of 0.8. ConclusionsWFC could slow down the progression of PLGC by regulating intestinal microbiota abundance. Trial registrationNCT03814629. Name of registry: Randomized Clinical Trial: Weifuchun Treatment on Precancerous Lesions of Gastric Cancer. Registered 3 August 2018-Retrospectively registered, https://register.clinicaltrials.gov/ NCT03814629.