bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification of ncRNA-mediated prognostic Value and Immune Infiltration of MTHFD2 in Bladder Cancer
Authors: Zhang Hao , Li Rui , Deng Wanjun , Xiong Huihua 
Published: November 2021

Abstract
Background: Methylenetetrahydrofolate dehydrogenase 2 (MTHFD2), one of mitochondrial enzymes, is involved in folate and nucleic acid metabolism and maintains the cellular redox balance. However, the function of MTHFD2 in bladder cancer is still poorly characterized. This study was designed to elucidate the effect and regulatory mechanism of MTHFD2 on bladder cancer cells and explore the relationships between MTHFD2 and immune cell infiltration in tumor microenvironment (TME). Methods: The data from Oncomine, TIMER, The Cancer Genome Atlas (TCGA) and The Human Protein Atlas (HPA) database were extracted to evaluate the expression of MTHFD2 and its prognostic role in pan-cancer, especially in bladder cancer. Enrichment analyses, were utilized to explore the underlying cellular mechanisms. The ncRNA regulatory axis was established by Starbase database, and the PPI network was constructed by Cytoscape software. Ultimately, the relations between the expression of MTHFD2 and immune cell infiltration in bladder cancer was indicated by TCGA and TIMER databases.Results: Our results demonstrated that MTHFD2 expression was generally up-regulated in pan-cancers and its high expression was correlated with poor prognosis of patients with bladder cancer. Specifically, our study revealed that MTHFD2 was a powerful risk factor and involved in the tumor development of bladder cancer. Furthermore, hsa_circ_0046140 and hsa_circ_0006769/miR-383-5p/MTHFD2 axis could also play a significant role in tumorigenesis. Ultimately, a strong correlation was observed between MTHFD2 expression and various immune cell infiltration, which implied that MTHFD2 might serve as an agent in tumor immunotherapy. Conclusion: MTHFD2 is widely overexpressed in pan-cancers, and its expression is related with the prognosis of patients and the multiple immune cell infiltrates in TME. Besides, hsa_circ_0046140 and hsa_circ_0006769/miR-383-5p/MTHFD2 axis are implicated with the proliferation and invasion of tumors.
Turning of Inconel 718 Using Liquid Nitrogen: Multi-objective Optimization of Cutting Parameters Using RSM
Authors: Eskandari Behzad , Bhowmick Sukanta , Alpas Ahmet 
Published: November 2021

Abstract
The objective of this research was to investigate the effectiveness of application of liquid nitrogen (LN2) in turning of Inconel 718 compared to flooded cutting and select suitable LN2 cutting parameters using response surface methodology (RSM). The results of turning experiments conducted by spraying LN2 to the cutting area of Inconel 718 bar showed that using either low or high cutting parameters, cutting performance of Inconel 718 under the cryogenic condition was generally worse than the flooded cutting. However, using the medium cutting parameters the cutting performance was as good as flooded cutting showing the values of 90 N of cutting force, 60 µm of flank wear and 0.5-0.6 µm of surface roughness (Ra). These parameters were further optimized using desirability function of RSM to determine the set of parameters that provide the lowest cutting force, flank wear and Ra and the highest material removal rate (MRR) under cryogenic cutting. Analysis of variance (ANOVA) performed on the developed regression models showed that cutting speed was the significant factor on cutting force. Feed rate was the most influential parameter on flank wear. Feed rate and depth of cut was significant factors affecting Ra. Multi-objective optimization showed that a cutting speed of 87 m/min, a feed rate of 0.06 mm/rev and a depth of cut of 0.37 mm constituted the optimum cutting parameters for achieving the, cutting force of 78 N, flank wear of 58 µm, Ra of 0.49 µm and the MRR of 1.97 cm3/min under cryogenic cutting condition.
Five-Lipoxygenase-Activating Protein Mediated CYLD Attenuation is a Candidate Driver in Hepatic Malignant Lesion
Authors: Su Kun-kai , Zheng Xue-hua , Bréchot Christian , Zheng Xiao-ping , Zhu Dan-hua , Huang Rong , Zhang Yan-hong , Tao Jing-jing , Lou Yi-jia , Li Lan-juan 
Published: November 2021

Abstract
Background: Hepatocellular carcinoma (HCC) is an inflammation-associated cancer. However, the lipid pro-inflammatory mediators have only been seldom investigated in HCC pathogenesis. Activation of NF-κB and expression of c-Myc are negatively regulated by cylindromatosis (CYLD) in hepatocarcinogenesis. But it remains largely unknown whether lipid pro-inflammatory mediators are involved in CYLD suppression. Here, we aimed to evaluate the significance of hepatic lipid pro-inflammatory metabolites of arachidonate affected CYLD expression via 5-lipoxygenase (5-LO)-pathway.Methods: Resection liver tissues from HCC patients or donors were evaluated for the correlation of 5-LO/cysteinyl leukotrienes (CysLTs)-signaling to expression of CYLD. The impact of functional components in 5-LO/CysLTs cascade on survival of HCC patients was subsequently assessed. Both livers from canines, a routine animal for drug safety evaluation, and genetic-modified human HCC cells treated with hepatocarcinogen aristolochic acid I (AAI) were further used to reveal the possible relevance between 5-LO pathway activation and CYLD depression. Results: 5-LO-activating protein (FLAP), an essential partner of 5-LO, significantly overexpressed and was parallel to CYLD depression, CD34 neovascular localization, and high Ki-67 expression in the resection tissues from HCC patients. Importantly, high hepatic FLAP transcription markedly shortened the median survival time of HCC patients after surgical resection. In the livers of AAI-treated canines, FLAP overexpression was parallel to enhanced CysLTs contents, simultaneous attenuated CYLD expression. Moreover, knock-in FLAP significantly diminished the expression of CYLD in AAI-treated human HCC cells.Conclusions: Hepatic FLAP/CysLTs axis is a crucial suppressor of CYLD in HCC pathogenesis, which highlights a novel mechanism in hepatocarcinogenesis and development. FLAP therefore can be explored for the early HCC detection and a target of anti-HCC therapy.
Source Apportionment and Source-specific Health Risk of Heavy Metals in Urban Road Dust of Jinan City, East China
Authors: Zhang Shengwei , Ma Ge , Dong Shuzhen , Meng Xiangzhou , Wang Lijun 
Published: November 2021

Abstract
For a better regional and source-risk-based control of heavy metals in urban environments, this study provides a source-specific health risk assessment by combining the models of United States Environmental Protection Agency health risk assessment and positive matrix factorization (PMF). The calculated data were optimized by the geochemical speciation of target 10 potentially toxic heavy metals. The results demonstrated that the mean concentrations of most heavy metals in urban dust of Jinan City exceeded their corresponding background values, especially that of cadmium (Cd) and zinc (Zn) exhibiting a mean of 12.9 and 7.84 times those of their backgrounds. Cd, Zn, copper, lead and manganese in road dust existed mainly in extractable forms, exhibiting higher bio-availability. The PMF receptor model determined four sources of heavy metals in urban road dust, namely industrial discharges (41.1%), natural and coal combustion sources (27.8%), traffic emissions (22.8%), and building material and manufacturing sources (8.3%). All the studied heavy metals presented low or negligible non-carcinogenic risk (non-CR) for adults and children, while the lifetime carcinogenic risk (CR) of Cd was in an acceptable level. Regarding source-specific health risks, the highest non-CR was derived from industrial discharges, while CR from traffic emissions, which were mainly associated with the higher content and bio-availability of Pb and Cd in the dust. Moreover, the risk contributions of industrial discharges and traffic emissions were 35.9% and 60.6% for non-CR and CR, respectively, presenting a significant difference with the apportioned source characteristics, thus deep-revealing the potentially source-based risks of heavy metal in urban environment.
Prevalence, Risk Factors and Prevention of Depression in the Adult Population in Mbarara District, Uganda
Authors: Allen‫٭‬ Kiconco , Laban Waswa Bright , Edward Ssemakula , Moses Baikirize , Muhwezi Talbert 
Published: November 2021

Abstract
Background. This study concerns the prevalence, risk factors and prevention of depression in the adult population in three sub-counties in Mbarara district. Depression is a common mental disorder which presents with a wide array of symptoms including loss of energy, poor sleep, poor eating habits, fatigue, irritability and social withdrawal. The main objective of the present study was to determine the prevalence and factors associated with depression and to identify measures that can be implemented to reduce risk factors of the disease among adults in Nyamitanga, Rugando and Rwanyamahembe sub-counties in Mbarara district. Methods. A cross-sectional design was used to capture data from a study sample size of 383 participants who were involved in completion of screening tests for depression. Scores from a 7-item version of Hopkins Symptom Checklist for Depression were averaged and the probable depression determined for each participant using a cut-off of 1.75. Logistic regression analyses were used to examine associations between depression outcomes and socio-demographic, behavioural and medical history variables. Results. The analyses revealed that the prevalence of depression among adults in the area stands at 27.7%. Depression was significantly associated with discrimination (AOR = 2.33, 95% CI = 1.22–4.45, P = 0.010), household size (AOR = 0.54, 95% CI = 0.32–0.93, P = 0.026), level of education (primary education: AOR = 0.36, 95% CI = 0.15–0.88, P = 0.024; secondary education: AOR = 0.30, 95% CI = 0.12–0.76, P = 0.011; tertiary education: AOR = 0.37, 95% CI = 0.15–0.92, P = 0.032) and drug-abuse related problems (AOR = 4.44, 95% CI = 1.34–14.74, P = 0.015). Measures for reducing the development of depression (which were identified by study participants) include guidance and counselling, awareness campaigns and self-help projects, improved access to quality medical services as well as stress management and spiritual care services. Conclusions. These findings show that depression prevalence in the study area, estimated at 27.7%, is high and worrying. Previous studies carried out in Mbarara district and other parts of Uganda have also reported similarly high estimates. They also show that discrimination, household size, level of education and drug-abuse are important risk factors associated with the prevalence of depressive disorders. Strategies targeted at early interventions and ultimately depression prevention need urgent attention in order to reduce risk factors of the disease.
Simulation of an Isolated System Behavior at High RES Penetration Coupled With Storage
Authors: Katsanevakis Athanasios , Konstantinidis Dimitris , Karagiannis Georgios , Ganias Athanasios , Karagiorgis George , Partasides George , Petrides Demetris , Ioannidou Maria 
Published: November 2021

Abstract
Τhe aim of the present work is to assess the overall benefits of applying electrical energy storage, especially to isolated grids, to harvest the underlying Renwable Energy Sourses potential sustainably. One such case is Cyprus, where due to various technical constraints related to the isolated nature of the island’s electricity system, RES in the electricity sector can reach a maximum level assuming limited curtailments, as early as 2023-2024. To this end, simulations have been set up and run using the DISPA-SET tool to investigate the potential of new electricity storage facilities at effective accommodation of high Renewable Energy Technologies (RET) penetration, especially photovoltaics in the coming years. Results show that particularly in isolated grids, RET penetration has to be coupled with storage to avoid power curtailment and provide security to the whole system, reduce the energy not served and provide a long-term perspective for the decarbonaization in the electricity sector towards 2050 zero-emission targets.
CCCH Zinc Finger Genes in Barley: Genome-Wide Identification, Evolution, Expression and Haplotype Analysis
Authors: Ai Qi , Pan Wenqiu , Zeng Yan , Li Yihan , Cui Licao 
Published: November 2021

Abstract
Background: CCCH transcription factors are important zinc finger transcription factors involved in the response to biotic and abiotic stress and physiological and developmental processes. Barley (Hordeum vulgare) is an agriculturally important cereal crop with multiple uses, such as brewing production, animal feed, and human food. The identification and assessment of new functional genes are important for the molecular breeding of barley. Results: In this study, a total of 35 protein-encoding CCCH genes unevenly dispersed on seven different chromosomes were identified in barley. Phylogenetic analysis categorized the barley CCCH genes (HvC3Hs) into seven subfamilies according to their distinct features, and this classification was supported by intron–exon structure and conserved motif analysis. Despite the large genome size of barley, the lower number of CCCH genes in barley might be attributed to the low frequency of segmental and tandem duplication events. Furthermore, the HvC3H genes displayed distinct expression profiles for different developmental processes and in response to various types of stresses. The expression of HvC3H9 was significantly induced by multiple types of abiotic stress and/or phytohormone treatment, which might make it an excellent target for the molecular breeding of barley. Genetic variation of HvC3Hs was characterized using publicly available exome-capture sequencing datasets. Clear genetic divergence was observed between wild and landrace barley populations in HvC3H genes. For most HvC3Hs, nucleotide diversity and the number of haplotype polymorphisms decreased during barley domestication. Conclusion: Overall, our study provides a comprehensive characterization of barley CCCH transcription factors, their diversity, and their biological functions.
Stratification of Prognoses based on Criteria from Clinical Trials of Metastatic Hormone-sensitive Prostate Cancer
Authors: Ahn Hyun Kyu , Yoo Jeong Woo , Koo Kyo Chul , Chung Byung Ha , Lee Kwang Suk 
Published: November 2021

Abstract
Background: Oncologic outcomes in metastatic hormone-sensitive prostate cancer (mHSPC) are extremely heterogeneous. We aimed to (1) stratify the prognosis in mHSPC patients according to criteria for high-volume disease, as defined in clinical trials, and (2) identify the combinations of unfavorable risk factors.Methods: This retrospective study reviewed 623 patients who were diagnosed with mHSPC between 1996 and 2014. The prognoses of mHSPC patients were stratified by criteria from the GETUG15, CHAARTED, STAMPEDE, and LATITUDE trials. The exclusion criteria were incomplete clinical data, docetaxel chemotherapy with upfront options, and metastatic disease without proper management after initial diagnosis.Results: All 485 patients (median follow-up=36.1 months) were categorized according to stage: M1a (70, 14.4%), M1b (367, 75.7%), and M1c (48, 9.9%). Significant differences in overall survival (OS) and cancer-specific survival (CSS) were found among the groups with low-volume disease, as classified by four clinical trials (log-rank p=0.001 and p<0.001, respectively). Bone metastasis volume and liver metastasis were independent predictors of prognosis. According to disease classification under NCCN guidelines, the prognosis of CSS between low-volume disease patients and M1c patients (no bone metastasis and low-volume bone metastasis) was not significantly different. Additionally, the prognosis of CSS did not significantly differ between M1c (high-volume bone metastasis and visceral metastasis, except liver) and M1b (high-volume bone metastasis) patients.Conclusions: The prognoses of patients with low-volume disease, based on several classification systems, were heterogeneous. Except for lung or liver metastasis, the combination of visceral metastasis with no/low-volume bone disease should be considered as a proxy of less aggressive disease in patients presenting with mHSPC.
Drug Associated Giant Cell Arteritis with Scalp Necrosis after Treatment with Pembrolizumab: A Case Report
Authors: Dargham Bassel Bou , Kang Julianna , Gavin Joshua , Abhishek Nandan 
Published: November 2021

Abstract
Giant cell arteritis (GCA) can be a rheumatologic complication of checkpoint inhibitor immunotherapy for the treatment of cancer. A rare and serious manifestation of GCA is scalp necrosis. We report a case of a patient who developed GCA and subsequent scalp necrosis after the initiation of immunotherapy with the checkpoint inhibitor pembrolizumab for the treatment of metastatic melanoma. Such advanced cases of GCA may be increasingly recognized in the immunotherapy era of oncology.
Engaging with EPIO, a digital pain self-management program: A qualitative study
Authors: Bostrøm Katrine , Varsi Cecilie , Eide Hilde , Børøsund Elin , Kristjansdottir Olof B , Schreurs Karlein MG , Waxenberg Lori B , Weiss Karen E , Morrison Eleshia J , Nordang Elise Flakk , Stubhaug Audun , Nes Lise Solberg 
Published: November 2021

Abstract
Background:Chronic pain conditions entail significant personal and societal burdens and improved outreach of evidence-based pain self-management programs are needed. Digital cognitive-behavioral self-management interventions have shown promise. However, evidence is still scarce and several challenges with such interventions for chronic pain exist. Exploring patients' experiences and engagement with digital interventions may be an essential step towards developing meaningful digital self-management interventions for those living with chronic pain. Objectives:This study aimed to gain insight into the experiences of people with chronic pain when engaging with EPIO, an application (app)-based cognitive-behavioral pain self-management intervention program.Methods: Participants (N=50) living with chronic pain received access to the EPIO intervention for 3 months. During this time, all participants received a follow-up phone call at 2-3 weeks, and a subsample (n=15) also participated in individual semi-structured interviews after 3 months. A qualitative design was used and thematic analysis was employed aiming to capture participants’ experiences when engaging with the EPIO intervention program. Results:Findings identifying program-related experiences and engagement were organized into three main topics, each with three sub-themes: (1) Engaging with EPIO; motivation to learn, fostering joy and enthusiasm, and helpful reminders and personalization, (2) Coping with pain in everyday life; awareness, practice and using EPIO, and (3) The value of engaging with the EPIO program; EPIO – a friend, making peace with the presence of pain, and fostering communication and social support. Conclusions: The current study explored participants’ experiences and engagement with EPIO, a digital self-management intervention program for people living with chronic pain. Findings highlighted identifying valued aspects related to motivation for engagement, incorporating content in support of daily coping, and encouraging a sense of acceptance and relatedness as vital components for facilitating program engagement.Trial Registration: ClinicalTrials.gov NCT03705104; https://clinicaltrials.gov/ct2/show/NCT03705104
Chemical quality and water quality indices of Fiche drinking water, Oromia, Ethiopia
Authors: Faye Gezahegn , Sebsibe Israel , Degaga Birhanu , Tekle Tsige 
Published: November 2021

Abstract
The problem of drinking water quality is common in developing countries. While a piped water supply is available in many parts of Fiche, Ethiopia, the water may be contaminated in the distribution system and thus continuous quality assessment is required to ensure safe drinking water for the community. Instrumental and spectrophotometric methods were used to analyze the chemical characteristics including dissolved oxygen, pH, temperature, chloride, fluoride, nitrate, nitrite, sulfate, ammonia, iron and manganese. The weighted arithmetic water quality index method was applied to assess the water's quality rating. The chemical characteristics of the tap water's annual range was; temperature between 19.4 and 20.0°C, pH between 6.8 and 7.6, DO between 4.5 and 6.1 mg/L, ammonia between 0.01 and 0.2 mg-NH3/L, nitrate between 1.1 and 1.8 mg-NO3−/L, nitrite between 0.003 and 0.06 mg-NO2−/L, 2.0 and 19.1 mg-SO42−/L, chloride between 10.07 and 30.0 mg-Cl−/L, manganese between 0.003 and 0.003 mg-Mn/L, iron between 0.01 and 0.03 mg-Fe/L. The WQIs for the reservoir and tap water were 25.031 and 40.676, respectively, so the tap water is rated ‘good’ for drinking.
THE BACHELARDIAN ONEIRIC CHILDHOOD
Authors: Sales Rodrigo Viana , Gomes Ana Laudelina Ferreira 
Published: November 2021

This text deals with the notion formulated by Gaston Bachelard of oneiric childhood, which refers to a permanent childhood that is renewed through active imagination and poetic reverie, promoting an articulation between memory and imagination in our awaken dreams. Aiming to give visibility and more reflection on the bachelardian oneiric childhood, we have investigated the approaches of this phenomenon within works of post-graduate students supervised by Ana Laudelina F. Gomes in the Research Group in which we participate - Mythos-Logos:religion, myth and spirituality. After doing a bibliographical research on thesis, dissertations and papers written by some of the members that are part of the group which works with such a notion, like Badiali (2016), Batista (2015, 2016, 2017, 2018), Eustáquio (2015, 2016), Melo (2012) and Gomes (2013b), it was noticed how this theme was recurring in a lot of different works. I perceived that reading these studies has triggered my own oneiric childhood, as it is in a ritual of anthropophagic readings. My infant being was fed by the narratives and images of those childhoods, which further expanded my poetic reveries, experience and reflection on the theme. Therefore, I have decided to present within this article some of the repercussions these readings provoked in me, stems from the collective orientation given by the supervisor Professor and sometimes by an invited person, when all the postgraduate students involved in the research read and make comments on the studies of one another.

Keywords: Childhood; Gaston Bachelard; Oneiric Poetry; Anthropophagic Readings;
Planejamento turístico: aspectos teóricos e conceituais e suas relações com o conceito de turismo
Authors: Binfare Paula Wabner , Castro Cleber Trindade , Silva Michel Vieira , Galvão Patrícia Lins , Costa Sinthya Pinheiro 
Published: November 2021

A atividade turística ganha espaço de reflexões no meio acadêmico ao longo dos anos e a cada novo passo que se dá, maior o cabedal de teorias oriundas de estudiosos do assunto. O Planejamento Turístico é revisado à medida que novos conhecimentos vêm sendo incorporados por pesquisadores e estudiosos do turismo. Neste trabalho são discutidos aspectos conceituais relacionados às teorias sobre turismo e planejamento turístico, destacando o panorama conceitual dessas categorias em publicações brasileiras que proponham as etapas em que o planejamento do turismo deveria ocorrer. Utilizou-se os procedimentos metodológicos de levantamento bibliográfico e análise do conteúdo das principais obras que tratam do planejamento turístico por autores Brasileiros, a saber: Barretto (1991), Ruschmann (1997), Beni (1998) e Dias (2003). Os resultados demonstram que as etapas de planejamento, propostas pelos autores em questão se relacionam com o entendimento que cada autor tem da atividade turística.