bluuebunny/crossref_metadata_2025_split huggingface dataset:
Ease and Safety of Securing Airway by Railroading ProSeal Laryngeal Mask Airway over Bougie versus Traditional Digital Insertion Technique
Authors: Tosh Pulak , Rajan Sunil , Narayani Naina , Kumar Lakshmi 
Published: November 2021

Background:
ProSeal insertion is usually more time-consuming with lower first-attempt success.

Aim of the Study:
We aimed to compare the incidence of successful placement of ProSeal laryngeal mask airway (PLMA) in the first attempt by railroading it over soft gum-elastic bougie introduced into esophagus versus traditional digital insertion technique. Time taken and ease of securing airway, associated hemodynamic responses, and trauma were also assessed.

Settings and Design:
This was a prospective randomized study conducted in a tertiary care institute.

Subjects and Methods:
Forty patients were recruited into two groups. Following induction in Group P, PLMA was introduced using digital technique, whereas in Group B, a soft gum-elastic bougie was introduced into esophagus and then PLMA was railroaded over the bougie. Correct placement was confirmed with end-tidal carbon dioxide waveform.

Statistical Tests Used:
Fisher's exact test and independent samples t-test were used for statistical analysis.

Results:
Successful first-attempt insertion was significantly higher in Group B compared to Group P (100% vs. 70%). Percentage of easy insertion was also significantly higher in Group B (95% vs. 65%). Time taken to insert ProSeal was significantly shorter in Group B (30.8 ± 7.8 vs. 59.5 ± 44.6 s). No patient in Group B had blood-stained secretion versus 70% in Group P. Mean arterial pressures at and after ProSeal insertion were significantly higher in Group P. However, heart rate remained comparable in both the groups.

Conclusion:
Bougie-guided ProSeal insertions had significantly higher first-attempt insertion success rates and were significantly faster and less traumatic with blunted blood pressure response compared to traditional digital insertion technique.
Regulated upon activation, normal T cells expressed and secreted (CCL5) in platelet concentrate
Authors: Shukla Rinku V. , Mody H. , Gupte Snehalata C. , Ghosh Kanjaksha 
Published: November 2021

BACKGROUND AND OBJECTIVES:
Platelet concentrates (PCs) can be prepared in several different ways, and they can be stored over few days before the use. Regulated on activation, normal T cells expressed and secreted (RANTES) levels in these concentrates may vary depending on the type of preparation and duration of storage of this component. We measured RANTES levels in platelet supernatants in different preparations and with different storage duration.

MATERIALS AND METHODS:
Fifteen PCs were prepared by platelet-rich plasma (PRP) and buffy coat (BC) method each. Forty-two single donor platelets (SDPs) were prepared using cell separators Cobe Spectra, Trima Accel, and Amicus. Filtered PCs were prepared using labside and bedside filters. The supernatants were collected after 1, 18, 65, and 112 h of preparation. SDP samples were taken on the 0 day, 3rd day, and 5th day. In filtered PC, pre- and post-filtration samples were taken, and aliquots were frozen at − 56°C for the measurement of RANTES.

RESULTS:
RANTES at 1 h was 1210 ± 560 pg/ml in PRP-PC, 1384 ± 463 pg/ml in BC-PC. At 112 h, 1617 ± 451 pg/ml and 1949 ± 134 pg/ml, respectively. In SDP, 0-day level was 1850 ± 278 pg/ml and >2000 pg/ml on 5th day. In prestorage, filtered PC RANTES was 1035 ± 496 pg/ml, and in the poststorage sample, it was 310 ± 508 pg/ml. With bedside filters, presample showed 1243 ± 832 pg/ml and postsample showed 556 ± 748 pg/ml.

CONCLUSION:
The concentration of RANTES increased continuously from 1 h to 5 days of storage in all PCs. After 65 h, BC-PC showed higher levels of RANTES compared to PRP-PC. Filtered PRP-PCs appear to be the best in terms of low RANTES to prevent allergic reactions and cultures negative.
Frequency of hepatitis B virus-DNA among hepatitis B surface-Ag negative, anti-hepatitis B core antibody-positive blood donors in Yazd, Iran
Authors: Vaziri Mahtab , Shahshahani Hayedeh Javadzadeh 
Published: November 2021

BACKGROUND:
The diagnosis of hepatitis B infection in most blood transfusion centers is based on hepatitis B surface (HBs) antigen detection by an enzyme immunoassay method. This study aimed to determine the frequency of hepatitis B core (HBc) antibody, HBs antibody, and hepatitis B virus (HBV) DNA among HBs antigen-negative healthy blood donors of Yazd province, Iran.

MATERIALS AND METHODS:
This cross-sectional study was conducted on 1500 healthy blood donor samples negative for HBs antigen, hepatitis C virus antibody, human immunodeficiency virus antigen/antibody, and rapid plasma regain tests. All samples were screened for HBc antibody test. HBs antibody and real-time polymerase chain reaction were performed for HBc antibody-positive samples.

RESULTS:
HBc antibody was positive in 74 (4.9%) samples and 11 (14.9%) of 74 positive samples for HBc antibody were negative for HBs antibody. Sixty-three (85.1%) positive samples for HBc antibody had HBs antibody titer over 10 IU/L, and 43 (58.1%) had HBs antibody titer over 100 IU/L. There was no hepatitis B DNA-positive sample in the present study.

CONCLUSIONS:
The study results suggest that there is a very low risk for transmission of HBV through blood donors of Yazd, Iran.
Molecular and serological markers of human parvovirus B19 infection in blood donors
Authors: Farahmand Mohammad , Tavakoli Ahmad , Ghorbani Saied , Monavari Seyed Hamidreza , Kiani Seyed Jalal , Minaeian Sara 
Published: November 2021

BACKGROUND:
Human parvovirus B19 (B19V) is one of the blood-borne viruses. The virus can be transmitted to susceptible individuals by blood or blood products. The virus is not associated with significant disease in general population, while people with underlying problems such as immunodeficiency can cause anemia and arthritis. The current systematic review and meta-analysis aimed to estimate the overall prevalence of B19V DNA, anti-B19V IgG, and anti-B19V IgM antibodies in blood donors worldwide.

METHODS:
A systematic search was carried out in online databases for relevant studies from inception until March 30, 2019. Study selection was performed based on predesigned eligibility criteria. The proportion of B19V DNA, anti-B19V IgG, and anti-B19V IgM antibodies were pooled using the inverse variance method. All statistical analyses were performed using the R version 3.5.3, package “meta.”

RESULTS:
According to the random-effects model, the pool prevalence of B19V DNA, anti-B19V IgM, and anti-B19V IgG among blood donors was calculated to be 0.4% (95% confidence interval [CI] =0.3%–0.6%), 2.2% (95% CI = 1.3%–3.7%), and 50.1% (95% CI = 43.1%–57.1%), respectively.

CONCLUSION:
For the transmission of B19V through blood, the presence of the virus genome is required, and the present study showed that the prevalence of the virus genome in blood donors is <1%. Therefore, there is no need to screen donated blood for B19V infection.
An insight into the utilization of allogenic blood transfusion and factors affecting blood transfusion in total knee replacement surgery in a tertiary care hospital in Eastern India
Authors: Das Sudipta Sekhar , Kamilya Ranjan , Biswas Rathindra Nath , Ghosh Susanta 
Published: November 2021

BACKGROUND:
Significant blood loss and requirement of allogenic blood transfusion during or after total knee replacement (TKR) have been reported. Incidence of blood transfusion in TKR is highly variable and depends on several factors. We investigated the blood utilization in patients undergoing TKR in our hospital and depicted the important risk factors that determine the need of allogenic blood transfusion in primary unilateral TKR.

MATERIALS AND METHODS:
The study included 1241 consecutive patients undergoing primary unilateral total knee arthroplasty. All the surgeries were performed by a single surgical team of orthopedists following standard procedure. Patient and disease details were obtained from patient file and hospital information system. Compatibility test was performed in blood bank before blood reservation following mandatory guidelines. Details of test, blood issue, and blood transfusion were documented in the blood bank.

RESULTS:
Of 1241 enrolled patients, 1069 (86.2%) were female. The median age of patients was 66 years with mean preoperative hemoglobin of 9.9 g/dL. Allogenic blood transfused was needed in 223 (17.9%) patients. Diabetes mellitus, hypertension, thyroid disorders, and chronic heart diseases were the major comorbid conditions. Risk factors such as gender, American Society of Anesthesiologists score, preoperative hemoglobin, and intraoperative and postoperative blood losses were significantly associated with blood transfusion.

CONCLUSION:
The risk factors determining blood transfusion in TKR vary between studies, however, all centers should establish standard operating procedures describing the surgical procedure and transfusion support in TKR. In addition, each center may develop specific blood management strategy to rationalize blood transfusion in TKR and overall successful care in TKR.
Effect of prolonged storage at 2°C–6°C for 120 h on the coagulation factors of thawed cryoprecipitate
Authors: Marik Arghyadeep , Philip Joseph , Mallhi Rajiv Singh , Kushwaha Neerja , Kumar Sudeep , Biswas Amit Kumar , Yadav Arvind Kumar 
Published: November 2021

BACKGROUND:
Cryoprecipitate helps in replenishing important coagulation factors like fibrinogen, Factor VIII and von Willebrand factor without running the risk of volume overload. It is very useful in the treatment of trauma patients with active bleeding and works best when administered early. Extending the shelf life of thawed cryoprecipitate beyond 4 hours enables us to manage inventory better, reduces the burden of demand vs supply as well as minimizes wastage. It can also help in logistically supporting the transfusion services in making cryoprecipitate readily available in mass casualty scenarios (war, natural calamity) in remote locations by reducing the time required for thawing cryoprecipitate and the need for costly storage equipment. AIM: The aim of this study was to compare the levels of Factor VIII, Fibrinogen and von Willebrand factor on thawed cryoprecipitate after prolonged storage for 5 days at a temperature of 2-6°C.

METHODOLOGY:
The above mentioned coagulation factors were analyzed in cryoprecipitate at the time of product thaw and again after 120 hours of 2 to 6°C storage using fully automated coagulation analyser (STA Compact Max). All parameters were expressed as Mean ± Standard deviation and were analyzed using paired t-test with level of significance, P < 0.05.

RESULTS:
There was a significant decrease in the level of Factor VIII, whereas the levels of fibrinogen and von Willebrand Factor remained stable during the storage period. All the cryoprecipitate units retained factor activities above therapeutic range even after 5 days of storage at 2-6°C.

CONCLUSION:
Although the levels of clotting factors are reduced during storage, they are still maintained above the therapeutic range. In scenarios where maintaining frozen cryoprecipitate inventory is a logistical challenge and emergency massive demands of cryoprecipitate are foreseen, the use of pre-thawed cryoprecipitate can be considered as a viable option.
Analysis of red blood cell irradiation practices from South India
Authors: Murugesan Mohandoss , Augustine Merline , Nayanar Sangeetha Keloth 
Published: November 2021

INTRODUCTION:
Age of red blood cell (RBC) units at the time of irradiation is important and prolonged storage of preirradiated units is detrimental. The objectives were to determine RBC age at irradiation, days from expiry (DFE), and percentage of late transfusions of irradiated RBC. To estimate the concordance on expiry of irradiated RBC units with present American Association of Blood Banks (AABB)/Directorate General of Health Services (DGHS), New Delhi over British Committee for Standards in Hematology (BCSH) and Council of Europe (CE) guidelines.

METHODS:
All the RBC units irradiated for a 1 year period were included. Retrieved data included date of collection, irradiation, revised expiry, and issue of blood. Late transfusions are units transfused in the last 2 week of RBC's shelf-life and wastage due to expiry was determined. Chi-square and Kruskal–Wallis test were used for comparisons between the guidelines.

RESULTS:
Out of 1303 RBC units irradiated, the median age for irradiation was 2 (0–36) days and 99.3% units irradiated within day +14. Median DFE for these units transfused was 26 (0–28) days. 2.8% units expired as per local standards. Late transfusions happened in 121 (9.3%) units transfused. AABB/DGHS practice was not concordant with CE standards for 86 (6.6%) units and with BCSH 94 (7.2%) units. Overall discordance between the present practices was CE and BCSH was seen in 130 (10%) events.

CONCLUSION:
Median RBC irradiation age and DFE was two and 26 days respectively at our center. Only 90% concordance was observed between AABB/DGHS and CE/BCSH guidelines with 9.3% units transfused as late transfusions. Restricting late transfusions of irradiated RBC can act as surrogate to improve the quality of units transfused through an inexpensive strategy.
Irradiating stored blood and storing irradiated blood
Authors: Balasubramanyam Pramanya , Basavarajegowda Abhishekh , Hanumanthappa Nandeesha , Negi Vir Singh , Harichandrakumar K. T. 
Published: November 2021

INTRODUCTION:
Gamma Irradiation of blood products accentuates biochemical changes in the blood stored at 4°C. This study tried to compare the changes in potassium, sodium, glucose, lactate, and lactate dehydrogenase (LDH) levels in packed red blood cell (PRBC) units irradiated at various time points and then stored versus those stored for a particular period then irradiated.

METHODOLOGY:
One hundred and eighty units of RBCs were randomly assigned equally to be irradiated or not. Eighteen units each were irradiated by gamma irradiator using cobalt 60 (BI 2000) on day 1, 7, 14, 21, and 28 of their storage, respectively, in the irradiation group. All the units were assessed for their plasma levels of potassium, sodium, glucose, LDH, and lactate by clinical chemistry auto analyzer Beckman coulter AU680 weekly. The values were documented and analyzed by SPSS.

RESULTS:
Baseline values on day 1 for studied biochemical parameters were comparable between irradiated and nonirradiated groups. Mean values of potassium, lactate and LDH were higher in irradiated than nonirradiated PRBC bags. In contrast, Sodium and Glucose mean values were lower than baseline values. Maximum cumulative mean values were noted in day-21 irradiated bags when the parameters were measured on day-28 for potassium and lactate levels. This was followed by day 14 irradiated bags, followed by day 7 irradiated bags.

CONCLUSION:
The study indicates that irradiation of red cells later in their storage period had comparatively more detrimental changes in relation to potassium and lactate than irradiation in earlier days. Consideration of irradiation to be performed as close to the issue as possible to reduce a lesser number of days of storage postirradiation is to be explored.
Buffy coat pooled platelet concentrate
Authors: Kumar Rakesh , Dhawan Hari Krishan , Sharma Ratti Ram , Kaur Jyotdeep 
Published: November 2021

BACKGROUND:
Buffy coat pooled platelet concentrate (BCPP) is a new blood component mainly used in Europe, which has good attributes of both random donor platelets and apheresis platelets in terms of high platelet count, leukoreduced and available in emergency. We planned this study to compare quality parameters and biochemical activation markers among buffy coat pooled platelets and apheresis platelet concentrate (AP-PC) to establish the quality and safety of this new blood component during storage.

MATERIALS AND METHODS:
Three different preparations of BCPP were prepared: Nonleukoreduced (BCPP Part A), leukoreduced (BCPP Part B), and leukoreduced with platelet additive solution (PAS) (BCPP Part C) using a pool of 15 ABO-matched, nonreactive buffy-coats in each experiment to avoid any donor-related variations. Ten such experiments were done. Each BCPP was equivalent to 5 buffy coat units. Ten apheresis platelets were taken as control. Serial samplings were done on day 0, 3, and 5 of collection and were assessed for: volume, platelet count, white blood cell count, swirling, pH, sterility, glucose, lactate, soluble p-selectin, Interleukin (IL)-6, IL-1 β, and tumor necrosis factor alpha (TNF-α).

RESULTS:
BCPP Part C (leukoreduced with PAS) maintained the best quality parameters in terms of maintenance of pH, least lactate accumulation, least sP-selectin levels, and least accumulation of inflammatory mediators (IL-6, IL-1 β, and TNF-α) than the other groups >BCPP part B >AP-PC and >BCPP Part A. On day 5 of storage pH for BCPP Part A, Part B, Part C, and AP-PC was: 6.33, 6.42, 6.64, and 6.29, respectively, and soluble p-selectin (ng/ml) was 201 ± 22, 186 ± 11, 149 ± 18, 200 ± 23, respectively. BCPP Part B and AP-PC had comparable quality parameters and activation markers.

CONCLUSIONS:
Buffy coat pooled platelet has comparable and even better-quality control parameters (especially leukofiltered with PAS) than conventional platelet preparation and is a good alternative for meeting platelet transfusion requirements of critical patients during emergency hours in resource-constraint setting.
COVID-19 Pandemic and Mode Choice of Alabamian Truck Drivers’ Mobility to Grocery Shopping
Authors: OLUWOYE JACOB 
Published: November 2021

Background: In late December 2019, COVID-19, commonly referred to as the Coronavirus was identified in China because of the main explanation for recent human respiratory health cases. The virus was first detected in Wuhan City, and during a space of months, it had covered the whole globe. The virus has engendered huge drastic changes to world healthcare, economic, transportation, and education systems around the world.
Purpose: The general purpose of this study was to investigate the intersection of demographic characteristics and how truck drivers view change in their grocery shopping (CGS) under the COVID-19 circumstances of selected counties in Alabama. Specifically, the objectives of the study are to (1) examine if there is any relationship between marital status (MS) and CGS and (2) know and assess the choice of transport mode used for grocery shopping during the covid-19 pandemic
Methods: The research paper's goal necessitated the truck drivers’ views regarding commuting to the workplace. Following a summary of the literature review research phase, the researcher conducted a variety of semi-structured interviews with truck drivers in Alabama through Survey Monkey by a postgraduate student in June-July 2020. Overall, 50 truck drivers have completed the survey. The info was stored on Survey Monkey servers within the Center for Urban and Rural Research (CURR), Department of Community and Regional Planning, Alabama A&M University.
Results: The data analysis reveals their main workplace before the COVID-19 pandemic 94% of the truck drivers residing in Alabama especially from Jefferson county provided information about commuting to workplaces, while 4% to the places of educations (lecture room0 and a couple of production sites. Furthermore, 92% of the truck drivers reported NO change within the means of transport in commuting trips during the COVID-19 pandemic, while 8% indicated changes within the means of transport.
Implications: This research paper contributes important new empirical analysis of the truck drivers’ views regarding commuting to the workplace under the COVID-19 pandemic to some extent where there's an abundance of conceptual papers and opinion pieces but still scant evidence on the particular road safety of the pandemic for researchers to think about on potential person and situation factors related to COVID-19 that would affect road safety during and after the pandemic. Collaborative efforts by researchers and public and personal sectors are going to be needed to collect data and develop truck drivers' road safety strategies in reference to the new reality of COVID-19. Keywords: COVID‐19, health disparities, roadway safety, syndemics, truck driver
A ATUAÇÃO DO FISIOTERAPEUTA NA SAÚDE DO HOMEM: UM ESTUDO DE CASO
Authors: Larchet Rebeca Bonfim , Amorim Patrícia Brandão 
Published: November 2021

Introdução: A população masculina tornou-se uma grande preocupação dos serviços públicos de saúde, devido ao alto número de enfermidades graves e crônicas que afetam esse gênero, onde a falta de uma atenção à saúde adequada aumentam ainda mais esses agravos, além disso, quando comparado a mulher, o homem possui uma taxa de morbidade e mortalidade bem maior, por isso o Ministério da Saúde elaborou uma Política Nacional de Atenção Integral ao Homem. Dentro dessa política há atuação do profissional da Fisioterapia como integrante principal no processo de promoção, prevenção, promoção e reabilitação da saúde do homem desempenhando um papel fundamental. Objetivo: Diante disso, essa presente pesquisa tem como finalidade apresentar a atuação do Fisioterapeuta na Saúde do Homem, além de apresentar a importância desse profissional nesse grupo da população. Metodologia: A metodologia se dividiu em duas etapas, a primeira ocorreu uma revisão bibliográfica sobre o tema com a fundamentação em revisões bibliográficas, análises em sites confiáveis, leis e artigos científicos, na segunda etapa foi aplicado um questionário a 4 Fisioterapeutas que atendem no município de Nanuque, no Estado de Minas Gerais, onde foi abordado questões voltadas a visão e o papel desses profissionais frente a Saúde do Homem no seu município, sobre a Política Nacional de Atenção ao Homem, as principais doenças e as particulariedades desse público alvo. Conclusão: Ao término do trabalho ficou evidenciado que os objetivos propostos para o estudo foram suficientes, pois, foi possível identificar as diversas funções do Fisioterapeuta na Saúde do Homem.