bluuebunny/crossref_metadata_2025_split huggingface dataset:
Rapid COVID‐19 antigenic tests: Usefulness of a modified method for diagnosis
Authors: Soleimani Reza , Deckers Corentin , Huang Te‐Din , Bogaerts Pierre , Evrard Stéphanie , Wallemme Isaline , Habib Boutaina , Rouzé Pauline , Denis Olivier 
Published: May 2021

AbstractThe current reliable recommended test for coronavirus disease 2019 (COVID‐19) diagnosis is quantitative reverse‐transcription polymerase chain reaction (RT‐qPCR). Rapid antigen test devices could be useful as they are less expensive, faster without the need of specialized laboratories to perform the test. We report the performances of two rapid immunochromatographic antigen testing devices compared with RT‐qPCR for severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) detection in nasopharyngeal samples. We carried out a lateral‐flow tests study on 401 nasopharyngeal swab samples from nonduplicated suspected COVID‐19 subjects. An equal volume of universal transport medium tubes‐containing samples (dilution ratio = 1:15) were added to the manufacturer's extraction buffer solution (dilution ratio = 1:2) and analyzed on BioSpeedia COVID19Speed‐Antigen Test and on Abbott Panbio™ COVID‐19 Ag Rapid Test, devices. Qualitative results were compared to those obtained by the RT‐qPCR (Allplex™ SARS‐CoV‐2 Assay Seegene). Based on our data, the overall sensitivity for BioSpeedia and Panbio devices was estimated at 65.5% and 75.0%, respectively. The sensitivity was greater for cycle threshold values less than 25 achieving 90.4 and 96.8 for BioSpeedia and Panbio devices, respectively. A perfect specificity of 100.0% was observed for both devices.
The impact of teleconnections on the temporal dynamics in aboveground net primary productivity of the Mongolian Plateau grasslands
Authors: Jiao Cuicui , Yu Guirui , Yi Xiaobo , Zhao Wei , Xing Liwei , He Fan , Li Qiyu , Luo Ji 
Published: May 2021

AbstractTemporal dynamics in aboveground net primary productivity (ANPP) are significant for environmental protection and economic development in the Mongolian Plateau grasslands (MPGs). Climate control of ANPP's temporal variations has intensified in response to local meteorological conditions, while the contributions of large‐scale atmospheric circulations to ANPP variations are poorly understood in the MPGs. First, we explored the temporal dynamics in annual ANPP, and then, we analyzed correlations among annual ANPP, local meteorological variables, and three different teleconnections during 1982–2015 in this study. Our analytical results indicated that (a) ANPP exhibited an increasing trend during the entire study period. However, the increasing trend did not persist, and two reversals occurred in 1998 and 2007. (b) ANPP variations were primarily influenced by summer precipitation in most regions and by summer precipitation and temperature together in other regions of the MPGs. (c) The Atlantic Multidecadal Oscillation (AMO) in winter negatively dominated annual ANPP variations by influencing summer precipitation and summer temperature in central Mongolia. The North Atlantic Oscillation (NAO) in winter exerted strong positive impacts on ANPP variations by influencing summer precipitation in Inner Mongolia, China. The East Atlantic Western Russia (EAWR) in spring controlled annual ANPP variations by influencing summer precipitation in western and eastern Mongolia. (d) The winter AMO, winter NAO and spring EAWR significantly dominated the annual ANPP variations in 49.78% of the MPGs, with values of 18.90, 9.81 and 21.07%, respectively. (e) Reversals of the winter AMO, winter NAO and spring EAWR between negative and positive phases resulted in two reversals of ANPP trends. This study will improve our understanding of the role of large‐scale atmospheric circulations in regulating ANPP variations and will be valuable in guiding the development of grazing land management in Mongolia and Inner Mongolia, China.
Extreme precipitation trends in northeast Algeria using a high‐resolution gridded daily dataset
Authors: Bessaklia Hanene , Serrano‐Notivoli Roberto , Ghenim Abderrahmane Nekkache , Chikh Hamza Abdessamed , Megnounif Abdessalam 
Published: May 2021

AbstractThis work presents a study of variability and trends of extreme precipitation events in northeast Algeria using a high‐resolution gridded dataset. To this end, 10 extreme precipitation indices were computed over a daily gridded precipitation dataset with a spatial resolution of 1x1 km from 33 series of weather observatories, covering the period from 1970 to 2010. The processes involving the reconstruction of the missing data and gridding were applied based on the approach of creating reference values (RV), allowing for a comprehensive quality control of the original data while reporting and deleting suspicious values that do not correspond to the natural spatial and temporal distribution of daily precipitation. These RV were used to complete a reconstructed dataset without missing values, based on the cleaned data series. Then, it was used to create the high‐resolution gridded dataset over northeast Algeria. In view of the obtained results, a general increasing gradient of the extreme precipitation indices from south to north was noted, particularly at the coast and at high‐altitude regions. Furthermore, upward trends in extreme intensity and frequency precipitation indices, such as PRCPTOT, SDII, RX1day, RX5day, R10mm, R20mm, CWD, R95p, and R99p were observed, except for the consecutive dry days (CDD) index, which showed a remarkable decrease. However, most of the estimated trends were not statistically significant at .05 levels.
Prevalence and Features of Laryngopharyngeal Reflux in Patients with Primary Burning Mouth Syndrome
Authors: Lechien Jerome R. , Hans Stéphane , De Marrez Lisa G. , Dequanter Didier , Rodriguez Alexandra , Muls Vinciane , Ben Abdelouahed Fairouz , Evrard Laurence , Maniaci Antonino , Saussez Sven , Bobin Francois 
Published: May 2021

Objectives/HypothesisTo investigate the prevalence and features of laryngopharyngeal reflux (LPR) in patients with primary burning mouth syndrome (BMS).Study DesignProspective uncontrolled study.MethodsPatients who visited our Departments of Otolaryngology‐Head and Neck and Maxillofacial surgery with BMS were prospectively recruited from September 2018 to September 2020. Patients benefited from dental, maxillofacial, otolaryngological examinations, and hypopharyngeal–esophageal impedance pH‐monitoring (HEMII‐pH). Oral, pharyngeal, and laryngeal findings and symptoms were rated with Reflux Sign Assessment (RSA) and Reflux Symptom Score‐12 (RSS‐12). Patients were treated with a combination of diet, pantoprazole, and alginate.ResultsFrom the 81 included patients, 76 reported >1 pharyngeal reflux events (93.8%), accounting for 35 (46.1%), 24 (31.6%), and 17 (22.3%) acid, mixed, and nonacid LPR, respectively. Thirty‐two patients had both LPR and gastroesophageal reflux disease (GERD). Thirty‐eight patients benefited from pepsin saliva measurement, which was positive in 86.8% of cases. The mean scores of mouth/tongue burning, RSS‐12, and RSA significantly improved from pre‐ to post‐treatment (P < .004). At 3‐month post‐treatment, 62.5% of patients reported an improvement of mouth/tongue burning score. Patients with both GERD and LPR reported higher baseline RSS‐12 and RSA scores.ConclusionAcid, weakly acid, and nonacid LPR may be involved in the development of BMS. The use of an appropriate treatment considering the reflux features is associated with an improvement of symptoms and findings.Level of Evidence4 Laryngoscope, 131:E2627–E2633, 2021
Invasive Fungal Sinusitis in Post COVID‐19 Patients: A New Clinical Entity
Authors: El‐Kholy Noha Ahmed , El‐Fattah Ahmed Musaad Abd , Khafagy Yasser W. 
Published: May 2021

ObjectivesOccurrence of invasive fungal respiratory superinfections in patients with COVID‐19 has gained increasing attention in the latest studies. Yet, description of acute invasive fungal sinusitis with its management in those patients is still scarce. This study aims to describe this recently increasing clinical entity in relation to COVID‐19 patients.Study DesignLongitudinal prospective study.MethodsProspective longitudinal study included patients diagnosed with acute invasive fungal rhinosinusitis after a recent COVID‐19 infection. Antifungal agents given included amphotericin B, voriconazole, and/or posaconazole. Surgical treatment was restricted to patients with PCR negative results for COVID‐19. Endoscopic, open, and combined approaches were utilized to eradicate infection. Follow‐up for survived patients was maintained regularly for the first postoperative month.ResultsA total of 36 patients with a mean age of 52.92 ± 11.30 years old were included. Most common associated disease was diabetes mellitus (27.8%). Mycological analysis revealed infection with Mucor and Aspergillus species in 77.8% and 30.6% of patients, respectively. Sino‐nasal, orbital, cerebral, and palatine involvement was found in 100%, 80.6%, 27.8%, and 33.3% of patients, respectively. The most common reported symptoms and signs are facial pain (75%), facial numbness (66.7%), ophthalmoplegia, and visual loss (63.9%). All patients were treated simultaneously by surgical debridement with antifungal medications except for two patients with PCR‐positive swab for COVID‐19. These two patients received antifungal therapy alone. Overall survival rate was 63.89% (23/36).ConclusionClinical suspicion of acute invasive fungal sinusitis among COVID‐19 patients and early management with antifungal therapy and surgical debridement is essential for better outcomes and higher survival.Level of Evidence4 Laryngoscope, 131:2652–2658, 2021
Safety and Efficacy of Nano‐Pulse Stimulation Treatment of Non‐Genital, Cutaneous Warts (Verrucae)
Authors: Nuccitelli Richard , LaTowsky Brenda M. , Lain Edward , Munavalli Girish , Loss Lesley , Ross E. Victor , Jauregui Lauren , Knape William A. 
Published: May 2021

Background and ObjectivesThis study describes the effects of nano‐pulse stimulation (NPS) technology on the common verruca with the objectives of demonstrating efficacy and safety. NPS technology applies nanosecond pulses of non‐thermal electrical energy to induce highly localized regulated cell death in the cellular structures of the targeted zone with negligible effects on surrounding non‐cellular structures. Previous clinical studies applying NPS to common, benign skin lesions have demonstrated safety and efficacy in clearing seborrheic keratoses and sebaceous hyperplasia.Study Design/Materials and MethodsSixty‐two subjects were enrolled at a total of five sites. One hundred and ninety‐five study verrucae up to 10 mm wide were treated with NPS delivered by a console‐based handheld applicator (CellFX® System; Pulse Biosciences) and follow‐ups occurred every 30 days with the option to retreat at 30, 60, and 90 days. There were 62 untreated controls and 46% of the treated verrucae were recalcitrant.ResultsOverall, 75.3% (70/93) of the common verrucae, 72.7% (8/11) of the flat verrucae, and 43.8% (14/32) of the plantar verrucae treated with NPS were completely clear by 60 days following the last treatment and did not recur within the 120‐day observation period. The majority (54%) of verrucae cleared with a single NPS procedure. The most common treatment site reactions were erythema (50.5%) and eschar formation (23.4%) on Day 30 and on Day 120 mild erythema was present in 14% of the cases and hyperpigmentation in 18.5%. No serious adverse events were reported. A particle counter was used during 11 NPS procedures on verrucae and no significant plume generation was detected during these procedures.ConclusionsNPS is a safe and effective procedure for removing non‐genital, cutaneous verrucae. Lasers Surg. Med. © 2021 The Authors. Lasers in Surgery and Medicine published by Wiley Periodicals LLC.
Intronic Haplotypes in the GBA Gene Do Not Predict Age at Diagnosis of Parkinson's Disease
Authors: Toffoli Marco , Higgins Abigail , Lee Chiao , Koletsi Sofia , Chen Xiao , Eberle Michael , Sedlazeck Fritz J. , Mullin Stephen , Proukakis Christos , Schapira Anthony H.V. 
Published: May 2021

ABSTRACTBackground
GBA mutations are a common risk factor for Parkinson's disease (PD). A recent study has suggested that GBA haplotypes, identified by intronic variants, can affect age at diagnosis of PD.ObjectivesIn this study, we assess this hypothesis using long reads across a large cohort and the publicly available Accelerating Medicines Partnership–Parkinson's Disease (AMP‐PD) cohort.MethodsWe recruited a PD cohort through the Remote Assessment of Parkinsonism Supporting Ongoing Development of Interventions in Gaucher Disease study (RAPSODI) and sequenced GBA using Oxford Nanopore technology. Genetic and clinical data on the full AMP‐PD cohort were obtained from the online portal of the consortium.ResultsA total of 1417 participants were analyzed. There was no significant difference in age at PD diagnosis between the two main haplotypes of the GBA gene.Conclusions
GBA haplotypes do not affect age at diagnosis of PD in the two independent cohorts studied. © 2021 The Authors. Movement Disorders published by Wiley Periodicals LLC on behalf of International Parkinson and Movement Disorder Society
Sphingosine‐1‐Phosphate, Motor Severity, and Progression in Parkinson's Disease (MARK‐PD)
Authors: Schwedhelm Edzard , Englisch Catrin , Niemann Louisa , Lezius Susanne , von Lucadou Mirjam , Marmann Kristina , Böger Rainer , Peine Sven , Daum Günter , Gerloff Christian , Choe Chi‐un 
Published: May 2021

ABSTRACTBackgroundTreatment with sphingosine‐1‐phosphate (S1P) agonists confers neuroprotective effects in animal models of Parkinson's disease (PD).ObjectivesWe assessed the association of serum S1P levels with motor and cognitive symptoms in patients with PD.MethodsS1P concentrations were analyzed with liquid chromatography–tandem mass spectrometry (LC–MS/MS) in serum of 196 PD patients and in 196 age‐ and sex‐matched controls. Motor (Unified Parkinson's disease rating scale III [UPDRS III], Hoehn and Yahr) and cognitive (Montreal Cognitive Assessment [MoCA]) function were assessed at baseline. Follow‐up data was available from 64 patients (median [interquartile range], 513 [381–677] days).ResultsS1P levels were lower in PD patients compared with controls, that is 1.75 (1.38–2.07) and 1.90 (1.59–2.18) μmol/L, respectively (P = 0.001). In PD patients, lower S1P concentrations were associated with higher UPDRS III scores and Hoehn and Yahr stage. In the follow‐up cohort, S1P concentrations below the median were associated with faster motor decline (hazard ratio: 4.78 [95% CI, 1.98, 11.50]), but not with cognitive worsening.ConclusionsOur observations reveal an association of S1P with PD. © 2021 The Authors. Movement Disorders published by Wiley Periodicals LLC on behalf of International Parkinson and Movement Disorder Society
Sperm morphometry is affected by increased inbreeding in the Retinta cattle breed: A molecular approach
Authors: Terán Ester , Azcona Florencia , Ramón Manuel , Molina Antonio , Dorado Jesús , Hidalgo Manuel , Ross Pablo , Goszczynski Daniel , Demyda‐Peyrás Sebastián 
Published: May 2021

AbstractThe effect of inbreeding depression on sperm motility is well documented, but its influence on sperm morphometry has been scarcely examined to date. Here, we combined the use of computer‐assisted sperm morphometry analysis (CASMA) with a SNP‐based genomic approach to determine and characterize the effect of inbreeding on the sperm shape of a highly inbred cattle population. We determined seven morphometric parameters on frozen‐thawed sperm samples of 57 Retinta bulls: length (L, µm), width (W, µm), area (A, µm2), perimeter (P, µm), ellipticity (ELI; L/W), elongation (L−W)/(L + W) and perimeter‐to‐area shape factor (p2a; P2/4 × π × A). The comparison of highly inbred (HI) and lowly inbreed (LI) individuals based on runs of homozygosity (ROH) inbreeding values (F
ROH) showed no differences between groups. An additional two‐step unsupervised sperm subpopulation analysis based on morphometric parameters showed significant differences in the abundance of different sperm subpopulations between groups (p < 0.05). This analysis revealed that HI bulls harbored a higher percentage of narrow‐head sperm as opposed to the higher percentage of large‐ and round‐headed sperm detected in LI. A further genomic characterization revealed 23 regions differentially affected by inbreeding in both groups, detecting six genes (SPAG6, ARMC3, PARK7, VAMP3, DYNLRB2, and PHF7) previously related to different spermatogenesis‐associated processes.
Different dietary combinations of folic acid and vitamin B12 in parental diet results in epigenetic reprogramming of IGF2R and KCNQ1OT1 in placenta and fetal tissues in mice
Authors: Mahajan Aatish , Sapehia Divika , Bagga Rashmi , Kaur Jyotdeep 
Published: May 2021

AbstractGenomic imprinting is important for mammalian development and its dysregulation can cause various developmental defects and diseases. The study evaluated the effects of different dietary combinations of folic acid and B12 on epigenetic regulation of IGF2R and KCNQ1OT1 ncRNA in C57BL/6 mice model. Female mice were fed diets with nine combinations of folic acid and B12 for 4 weeks. They were mated and off‐springs born (F1) were continued on the same diet for 6 weeks postweaning and were allowed to mate. The placenta and fetal (F2) tissues were collected at day 20 of gestation. Dietary deficiency of folate (BNFD and BOFD) and B12 (BDFN) with either state of other vitamin or combined deficiency of both vitamins (BDFD) in comparison to BNFN, were overall responsible for reduced expression of IGF2R in the placenta (F1) and the fetal liver (F2) whereas a combination of folate deficiency with different levels of B12 revealed sex‐specific differences in kidney and brain. The alterations in the expression of IGF2R caused by folate‐deficient conditions (BNFD and BOFD) and both deficient condition (BDFD) was found to be associated with an increase in suppressive histone modifications. Over‐supplementation of either folate or B12 or both vitamins in comparison to BNFN, led to increase in expression of IGF2R and KCNQ1OT1 in the placenta and fetal tissues. The increase in the expression of IGF2R caused by folate over‐supplementation (BNFO) was associated with decreased DNA methylation in fetal tissues. KCNQ1OT1 noncoding RNA (ncRNA), however, showed upregulation under deficient conditions of folate and B12 only in female fetal tissues which correlated well with hypomethylation observed under these conditions. An epigenetic reprograming of IGF2R and KCNQ1OT1 ncRNA in the offspring was evident upon different dietary combinations of folic acid and B12 in the mice.
Examination of optimized protocols for pCASL: Sensitivity to macrovascular contamination, flow dispersion, and prolonged arterial transit time
Authors: Zhang Logan X. , Woods Joseph G. , Okell Thomas W. , Chappell Michael A. 
Published: May 2021

PurposePreviously, multi‐ post‐labeling delays (PLD) pseudo‐continuous arterial spin labeling (pCASL) protocols have been optimized for the estimation accuracy of the cerebral blood flow (CBF) with/without the arterial transit time (ATT) under a standard kinetic model and a normal ATT range. This study aims to examine the estimation errors of these protocols under the effects of macrovascular contamination, flow dispersion, and prolonged arrival times, all of which might differ substantially in elderly or pathological groups.MethodsSimulated data for four protocols with varying degrees of arterial blood volume (aBV), flow dispersion, and ATTs were fitted with different kinetic models, both with and without explicit correction for macrovascular signal contamination (MVC), to obtain CBF and ATT estimates. Sensitivity to MVC was defined and calculated when aBV > 0.5%. A previously acquired dataset was retrospectively analyzed to compare with simulation.ResultsAll protocols showed underestimation of CBF and ATT in the prolonged ATT range. With MVC, the protocol optimized for CBF only (CBFopt) had the lowest sensitivity value to MVC, 33.47% and 60.21% error per 1% aBV in simulation and in vivo, respectively, among multi‐PLD protocols. All multi‐PLD protocols showed a significant decrease in estimation error when an extended kinetic model was used. Increasing flow dispersion at short ATTs caused increasing CBF and ATT overestimation in all protocols.ConclusionCBFopt was the least sensitive protocol to prolonged ATT and MVC for CBF estimation while maintaining reasonably good performance in estimating ATT. Explicitly including a macrovascular component in the kinetic model was shown to be a feasible approach in controlling for MVC.
Effects of motion and retrospective motion correction on the visualization and quantification of perivascular spaces in ultrahigh resolution T2‐weighted images at 7T
Authors: Zong Xiaopeng , Nanavati Soham , Hung Sheng‐Che , Li Tengfei , Lin Weili 
Published: May 2021

PurposeMotion can strongly affect MRI image quality and derived imaging measures. We studied the effects of motion and retrospective motion correction (MC) on the visualization and quantitative measures of the perivascular space and penetrating vessel (PVSV) complex, an essential part of the glymphatic system, on high‐resolution T2‐weighted MRI images at 7T.MethodsMC was achieved by adjusting k‐space data based on head positions measured using fat navigator images. PVSV visibility and quantitative measures including diameter, volume fraction (VF), count, and contrast were compared between images with and without MC.ResultsWithout MC, VF, and count decreased significantly with increasing head rotation. MC improved PVSV visualization in all cases with severe motion artifacts. MC decreased diameter in white matter (WM) and increased VF, count, and contrast in basal ganglia and WM. The changes of VF, count, and contrast after MC strongly correlated with motion severity. MC eliminated the significant dependences of VF and count on rotation and reduced the inter‐subject variations of VF and count. The effect sizes of age and breathing gas effects on VF and count, and contrast increased in most cases after MC, while those on diameter exhibited inconsistent behavior.ConclusionsMotion affects PVSV quantification without MC. MC improves PVSV visibility and increases the statistical power of detecting physiological PVSV VF, count, and contrast changes but may have limited benefits for increasing the power for detecting diameter changes.
Exploring the knowledge of community‐based nurses in supporting parents of preterm babies at home: A survey‐based study
Authors: Petty Julia , Whiting Lisa , Fowler Cathrine , Green Janet , Mosenthal Alison 
Published: May 2021

AbstractAimThis study aimed to investigate the confidence levels, knowledge base and learning needs of community‐based nurses relating to the care of preterm babies and parents, to explore what education is required and in what format.DesignAn online survey methodology was used.MethodsA 32‐item questionnaire was distributed via social media platforms to community‐based nurses in Australia.ResultsDescriptive analysis was undertaken relating to knowledge base, confidence levels, previous training, learning and resource needs and barriers to education. It was deemed vital to expand confidence and knowledge in this area. Gaps in learning resources were identified and a need for more training in topics such as developmental outcomes, feeding, expected milestones, weight gain, growth trajectories and supporting parents. Online resources were the preferred format to teach key knowledge to community‐based health professionals, tailored to the specific features of preterm babies and support needs of parents.