bluuebunny/crossref_metadata_2025_split huggingface dataset:
A CRISPR/Cas9‐based method for seamless N‐terminal protein tagging in Saccharomyces cerevisiae
Authors: Kira Shintaro , Noda Takeshi 
Published: August 2021

AbstractProtein tagging is an effective method for characterizing a gene of interest. Tagging can be accomplished in vivo in Saccharomyces cerevisiae by chromosomal integration of a PCR‐amplified cassette. However, common tagging cassettes are not suitable for in situ N‐terminal tagging when we aim to preserve the gene's endogenous promoter. Existing methods require either two rounds of homologous recombination or a relatively complex cloning process to construct strains with N‐terminal protein tags. Here, we describe a simple CRISPR/Cas9‐based method for seamless N‐terminal tagging of yeast genes that preserves their endogenous promoter. This method enables the generation of N‐terminally tagged strains by introducing an expression vector containing the cas9 gene and a specific gRNA for cleaving the 5′ end of the target gene's protein‐coding sequence, along with donor DNA containing the tag sequence and homology arms. gRNA cloning was executed by inverse PCR instead of the conventional method. After verifying the tag, the Cas9 and gRNA expression plasmids were eliminated without using antibiotic‐containing medium. By this method, we generated strains that express N‐terminally tagged subunits of the TORC1 protein kinase complex and found that these strains are comparable to strains made by conventional methods. Thus, our method provides a cost‐effective alternative for seamless N‐terminal tagging in baker's yeast.
Why Sexual Orientation and Gender Identity Asylum?
Authors: Danisi Carmelo , Dustin Moira , Ferreira Nuno , Held Nina 
Published: August 2021

AbstractForced migration – no matter how we wish to define it – has been high in the political agendas and debates across the world for several decades. Forced migrants become claimants of international protection, or ‘asylum claimants’, and then find themselves trapped in convoluted, constraining and highly politicised systems. Often accused of being ‘bogus’ asylum claimants, they are also regularly accused of abusing the hospitality of the host country, violating countries’ borders and territorial sovereignty, and simply seeking economic benefits (Ford 2009; UNHCR 2007). Conversely, asylum legal instruments have been repeatedly criticised for inadequately addressing the rights and needs of asylum claimants, therefore preventing those with legitimate claims from being granted protection. These debates have more recently been rehashed in the context of the negotiations behind the Global Compact for Safe Orderly and Regular Migration, a non-legally binding agreement negotiated under the aegis of the United Nations (UN) and endorsed by the UN General Assembly. In this atmosphere of permanent politicised and humanitarian ‘crisis’ (McAdam 2014), a group warranting specific attention is constituted by those asylum claimants presenting a claim based on their sexual orientation or gender identity (SOGI).
Believing in Something Better: Our Recommendations
Authors: Danisi Carmelo , Dustin Moira , Ferreira Nuno , Held Nina 
Published: August 2021

AbstractThe previous chapters have offered an in-depth, empirically and theoretically informed analysis of a broad range of issues affecting SOGI asylum claimants and refugees in Europe, with a focus on Germany, Italy and the UK. This analysis has applied new insights to findings unearthed by previous research and shone light on issues that have so far been relatively neglected. But the question often posed to us as academics is ‘so what?’ To address this question, this chapter offers a range of recommendations addressed to decision-makers, policy-makers, governments, NGOs and service providers, aimed at improving the socio-legal framework that applies to SOGI asylum. These apply mainly at a domestic level, but also refer to the European level, where relevant, to offer proposals that recognise the intersections of national, European and international frameworks. As in the previous analysis, we are strongly guided by our participants’ voices, complemented by broader scholarly debates and our analysis and views of these. Our data and analysis thereof also shed light on broader issues in the asylum system, and many of the recommendations set out here would benefit non-SOGI asylum claimants and refugees as well. We hope they will be of wider value in this way.
The Policy and Guidance
Authors: Danisi Carmelo , Dustin Moira , Ferreira Nuno , Held Nina 
Published: August 2021

AbstractThe contours of international protection in EU member states are mainly determined by three elements: the 1951 Refugee Convention and the EU Common European Asylum System (CEAS), alongside international human rights law, including the 1950 ECHR (Chap. 3). While Germany and Italy are bound by the CEAS recast instruments, the UK has remained only bound by the 2003 Reception Directive, 2004 Qualification Directive and 2005 Procedures Directive. Yet, this and other type of legislative options do not, in themselves, necessarily produce considerable variations across the EU (Querton 2019), as many other factors contribute to different degrees of policy variation between EU member states. Amongst these factors, it is worth considering in particular geographical location, political context and internal governance structure, further contextualised by the statistics available.
Housing and Accommodation
Authors: Danisi Carmelo , Dustin Moira , Ferreira Nuno , Held Nina 
Published: August 2021

AbstractThe SOGI claimants we interviewed were often more or as concerned about their living conditions in the host country than they were with the asylum process. This was especially the case in the interviews in Germany and Italy, where issues around housing and accommodation often dominated the discussion, both in interviews with SOGI claimants and with professionals. For instance, Elias, a lawyer in Germany, told us that his clients were often less concerned about the legal procedure and more about ‘the problems around it. Less legally, less tangibly in relation to the procedure, but rather: “What about the accommodation situation?”; “How can I rebuild a life appropriate to my sexual orientation?”’.
Einleitung
Authors: Sell Madlen 
Published: August 2021

ZusammenfassungDer aus der austronesischen Sprachfamilie entlehnte Begriff „Amok“ leitet sich von dem malaiischen Wort „mĕngamok“ ab, das ins Deutsche übersetzt „wütend angreifen“ bedeutet. Mit den Schiffen der ersten Weltumsegler erreichte der fremdartig klingende und in Europa bis dahin gänzlich unbekannte Begriff vor rund 600 Jahren erstmals die westliche Welt.
Tau-jet signatures of vectorlike quark decays to heavy charged and neutral Higgs bosons
Authors: Dermisek Radovan , Lunghi Enrico , McGinnis Navin , Shin Seodong 
Published: August 2021

AbstractWe study 4b+ 2τand 4b+ 1τsignatures of heavy neutral and charged Higgs bosons originating from cascade decays of pair-produced new quarks. Decays of vectorlike quarks through heavy Higgses can easily dominate in the two Higgs doublet model of type-II, and the studied signatures are common to many possible decay chains. We design search strategies for these final states and discuss the mass ranges of heavy Higgs bosons and new quarks that can be explored at the Large Hadron Collider as functions of branching ratios in a model independent way. We further combine the results with a similar study focusing on decays which lead to a 6bfinal state and interpret the sensitivity to charged and neutral Higgs bosons and vectorlike quarks in the type-II two Higgs doublet model. We find that the LHC reach for their masses extends to well above 2 TeV in the case of an SU(2) doublet quark and to at least 1.8 TeV for a bottom-like SU(2) singlet quark in the whole range of tanβbetween 1 and 50.
The lattice of monomial clones on finite fields
Authors: Kreinecker Sebastian 
Published: August 2021

AbstractWe investigate the lattice of clones that are generated by a set of functions that are induced on a finite field $${\mathbb {F}}$$
F
by monomials. We study the atoms and coatoms of this lattice and investigate whether this lattice contains infinite ascending chains, or infinite descending chains, or infinite antichains.We give a connection between the lattice of these clones and semi-affine algebras. Furthermore, we show that the sublattice of idempotent clones of this lattice is finite and every idempotent monomial clone is principal.
Behavioral Strategies to Minimize Procedural Distress During In-Office Pediatric Tympanostomy Tube Placement Without Sedation or Restraint
Authors: Cohen Lindsey L. , Robbertz Abigail S. , England Laura J. 
Published: August 2021

AbstractThe purpose of this study was to evaluate behavioral strategies to minimize procedural distress associated with in-office tympanostomy tube placement for children without general anesthesia, sedation, or papoose-board restraints. 120 6-month- to 4-year-olds and 102 5- to 12-year-olds were treated at 16 otolaryngology practices. Mean age of children was 4.7 years old (SD = 3.18 years), with more boys (58.1%) than girls (41.9%). The cohort included 14% Hispanic or Latinx, 84.2% White, 12.6% Black, 1.8% Asian and 4.1% ‘Other’ race and ethnicity classifications. The in-office tube placement procedure included local anesthesia via lidocaine/epinephrine iontophoresis and tube placement using an integrated and automated myringotomy and tube delivery system. Behavioral strategies were used to minimize procedural distress. Anxiolytics, sedation, or papoose board were not used. Pain was measured via the faces pain scale-revised (FPS-R) self-reported by the children ages 5 through 12 years. Independent coders supervised by a psychologist completed the face, legs, activity, cry, consolability (FLACC) behavior observational rating scale to quantify children’s distress. Mean FPS-R score for tube placement was 3.30, in the “mild’ pain range, and decreased to 1.69 at 5-min post-procedure. Mean tube placement FLACC score was 4.0 (out of a maximum score of 10) for children ages 6 months to 4 years and was 0.4 for children age 5–12 years. Mean FLACC score 3-min post-tube placement was 1.3 for children ages 6 months to 4 years and was 0.2 for children age 5–12 years. FLACC scores were inversely correlated with age, with older children displaying lower distress. The iontophoresis, tube delivery system and behavioral program were associated with generally low behavioral distress. These data suggest that pediatric tympanostomy and tube placement can be achieved in the outpatient setting without anxiolytics, sedatives, or mechanical restraints.
The Social Policy Response to COVID-19 – The Failure to Help Vulnerable Children and Elderly People
Authors: Christensen Tom 
Published: August 2021

AbstractNorway’s handling of COVID-19 has been seen as a success. Vulnerable groups among young and elderly people, have however, not been part of this success. Their interests have been defocused in two ways. First, they have not been prioritized in the big picture, because the precautionary principle and health concerns, in particular the capacity questions, have dominated. Second, the more specific social policy measures have been indirect and not particular targeted vulnerable children, youths and old people, and had negative effects for them, such as social isolation and lack of daily support and services, resulting in increasing problems.
Prevalence and Impact of Treatment-Resistant Depression in Latin America: a Prospective, Observational Study
Authors: Soares Bernardo , Kanevsky Gabriela , Teng Chei Tung , Pérez-Esparza Rodrigo , Bonetto Gerardo Garcia , Lacerda Acioly L. T. , Uribe Erasmo Saucedo , Cordoba Rodrigo , Lupo Christian , Samora Aline Medeiros , Cabrera Patricia 
Published: August 2021

AbstractApproximately one-third of patients with major depressive disorder (MDD) have treatment-resistant depression (TRD). The TRAL study will evaluate the prevalence and impact of TRD among patients with MDD in four Latin American countries. In this multicenter, prospective, observational study, patients with MDD were recruited from 33 reference sites in Mexico, Colombia, Brazil, and Argentina. Patients were assessed for TRD, defined as failure to respond to ≥ 2 antidepressant medications of adequate dose and duration. Demographics, previous/current treatments, depressive symptoms, functioning, healthcare resource utilization, and work impairment were also collected and evaluated using descriptive statistics, chi-square test, Fisher exact test, t-test for independent samples, or the Mann–Whitney nonparametric test, as appropriate. 1475 patients with MDD were included in the analysis (mean age, 45.6 years; 78% women); 89% were receiving relevant psychiatric treatment. 429 patients met criteria for TRD, and a numerically higher proportion of patients with TRD was present in public versus private sites of care (31% vs 27%). The mean Montgomery-Asberg Depression Rating Scale score was 25.0 among all MDD patients and was significantly higher for patients with TRD versus non-TRD (29.4 vs 23.3; P < 0.0001). Patients with TRD, versus those with non-TRD, were significantly more likely to be older, have a longer disease duration, have more comorbidities, be symptomatic, have a higher median number of psychiatric consultations, and report greater work impairment. Patients with TRD have a disproportionate burden of disease compared to those with non-TRD. Appropriate treatment for TRD is a substantial unmet need in Latin America. https://www.ClinicalTrials.gov identifier NCT03207282, 07/02/2017.
Citizens' Perception of Combined Sewer Overflow Spills into Bathing Coastal Areas
Authors: Martínez-Gomariz Eduardo , Guerrero-Hidalga Maria , Forero-Ortiz Edwar , Gonzalez Susana 
Published: August 2021

AbstractOnce a wastewater treatment plant (WWTP) exceeds its capacity, it is necessary to discharge a proportion of the flow to watercourses through combined sewer overflow (CSO) structures. In coastal urban areas, CSO spills may occur in seawaters. The present study analyses the effects of these spills into urban coastal bathing areas, through a qualitative survey-based analysis in Badalona and Barcelona, focusing on stakeholders' reputation and image involved in the sewer system and beaches management (i.e. intangible damages) and the consequences for the economy (i.e. tangible damages). The direct relation between CSO spills and impacts on restaurants’ revenues is not observed since business owners in Badalona and Barcelona do not perceive any economic impact due to these events. Their main concern is the municipality’s image, which might affect the citizens’ view of the management of the responsible agents and indirectly, the tourist sector, especially in Barcelona. Residents perception in this matter is remarkably different in both cities. In Badalona, residents know the problem and even the body responsible for setting the red flag and the beaches closure (i.e. the municipality). In Barcelona, though, residents are quite confused about this. The complexity in terms of competencies in sewer systems management requires a better communication campaign for the citizens to avoid misunderstandings and unnecessary loss of trust in the City Council. Decision-makers and stakeholders should be interested in understanding the perception of affected users upon these events to take appropriate measures to enhance awareness programs or measures to reduce overflows.
Can the sustainable development goals harness the means and the manner of transformation?
Authors: Veland Siri , Gram-Hanssen Irmelin , Maggs David , Lynch Amanda H. 
Published: August 2021

AbstractThe 17 sustainable development goals and their 169 targets comprise a comprehensive list of prerequisites for human and planetary well-being, but they also implicitly invoke many of the very trade-offs, synergies, and parallelisms that drive global crises. Decision-makers are familiar with these internal conflicts, and there is no shortage of frameworks, blueprints, and roadmaps to accelerate sustainability. However, thus far, inevitable trade-offs among competing priorities for sustainability are not catalyzing the types of transformations called for, indeed, demanded, by the SDGs. Habitual technocratic approaches, which the SDG lend themselves to, will report on indicators and targets, but will not adequately represent the ambitions of the goals themselves. Addressing these habitual tendencies, this paper therefore considers the inner dimensions of transformation, including emotions and meaning-making. Music offers a rich source of metaphor to reimagine interconnections and communicates affectively the feelings and embodied dimensions of intellectual thought and creativity. We draw on Western musical composition and history to offer insights on an intellectual path-dependency leading up to the current disembodied indicator-based management and regulation of global environmental and societal crises, and on potential alternatives. As metaphors, we consider what the SDGs might ‘sound like’ as either 12-tone, contrapuntal, or improvisational expression. We suggest that for the SDGs to release their transformative potential, ‘sustainability improvisers’ with a handle on both the ‘what’ and the ‘how’ of transformation are needed: harnessed with deep understanding of SDG indicators and targets, but with an ability to listen deeply and invite others to co-create transformative pathways.
The Prime Function, the Fay Trisecant Identity, and the van der Pauw Method
Authors: Miyoshi Hiroyuki , Crowdy Darren , Nelson Rhodri 
Published: August 2021

AbstractThe van der Pauw method is a well-known experimental technique in the applied sciences for measuring physical quantities such as the electrical conductivity or the Hall coefficient of a given sample. Its popularity is attributable to its flexibility: the same method works for planar samples of any shape provided they are simply connected. Mathematically, the method is based on the cross-ratio identity. Much recent work has been done by applied scientists attempting to extend the van der Pauw method to samples with holes (“holey samples”). In this article we show the relevance of two new function theoretic ingredients to this area of application: the prime function associated with the Schottky double of a multiply connected planar domain and the Fay trisecant identity involving that prime function. We focus here on the single-hole (doubly connected, or genus one) case. Using these new theoretical ingredients we are able to prove several mathematical conjectures put forward in the applied science literature.
US Consumers’ Online Shopping Behaviors and Intentions During and After the COVID-19 Pandemic
Authors: Jensen Kimberly L. , Yenerall Jackie , Chen Xuqi , Yu T. Edward 
Published: August 2021

AbstractA study of 1,558 US households in June 2020 evaluated utilization of online grocery shopping during the COVID-19 pandemic, influences on utilization, and plans for future online grocery shopping. Nearly 55 percent of respondents shopped online in June 2020; 20 percent were first-timers. Cragg model estimates showed influences on online shopping likelihood and frequency included demographics, employment, and prior online shopping. Illness concerns increased likelihood, while food shortage concerns increased frequency of online shopping. A multinomial probit suggested 58 percent respondents planned to continue online grocery shopping regardless of pandemic conditions.
Association Between Immediacy of Citations and Altmetrics in COVID-19 Research by Artificial Neural Networks
Authors: Ran Na 
Published: August 2021

Abstract

Objectives:
Both citations and Altmetrics are indexes of influence of a publication, potentially useful, but to what extent that the professional-academic citation and media-dominated Altmetrics are consistent with each other is a topic worthy of being investigated. The objective is to show their correlation.

Methods:
DOI and citation information of coronavirus disease 2019 (COVID-19) researches were obtained from the Web of Science, its Altmetric indicators were collected from the Altmetrics. Correlation between the immediacy of citation and Altmetrics of COVID-19 research was studied by artificial neural networks.

Results:
Pearson coefficients are 0.962, 0.254, 0.222, 0.239, 0.363, 0.218, 0.136, 0.134, and 0.505 (P < 0.01) for dimensions citation, attention score, journal impact factor, news, blogs, Twitter, Facebook, video, and Mendeley correlated with the SCI citation, respectively. The citations from the Web of Science and that from the Altmetrics have deviance large enough in the current. Altmetric score is not precise to describe the immediacy of citations of academic publication in COVID-19 research.

Conclusions:
The effects of news, blogs, Twitter, Facebook, video, and Mendeley on SCI citations are similar to that of the journal impact factor. This paper performs a pioneer study for investigating the role of academic topics across Altmetric sources on the dissemination of scholarly publications.
Predicting Intensive Care Unit Admissions for COVID-19 Patients in the Emergency Department
Authors: Bahadirli Suphi , Kurt Erdem 
Published: August 2021

AbstractObjective:Determining the parameters that can predict the requirement of intensive care unit (ICU) admissions among the coronavirus disease 2019 (COVID-19) patients presented to the emergency departments (EDs).Methods:In adult consecutive patients admitted (March 15 - April 15, 2020) to the ED of a state hospital for COVID-19, we retrospectively analyzed demographic data, symptoms, laboratory tests, and chest computed tomography (CT) on arrival.Results:We included 458 patients [213 (46.5%) females, median age 48 y]. Body temperature, respiration rate, C-reactive protein (CRP), D-dimer, ferritin values, and the number of comorbidities were significantly higher in patients admitted to the ICU than others. Also, diffuse infiltration in chest CT is more common in patients who need ICU follow-up. As a result of the binary regression analysis, a statistically significant correlation was found between the presence of dyspnea (odds ratio [OR]: 12.55), tachypnea (relative risk [RR] ≥ 18) (OR: 14.54), multiple comorbidities (≥2) (OR: 23.39), diffuse infiltration in CT (OR: 14.52), and CRP (≥45 mg/L) (OR: 4.71); and the need for ICU admission.Conclusion:It has been concluded that the presence of dyspnea and tachypnea, elevated CRP, presence of multiple comorbidities, and diffuse infiltration in CT may predict the need for ICU admissions of the patients, who presented to the EDs.
The Paradox of Empathy
Authors: Paul L.A. 
Published: August 2021

AbstractA commitment to truth requires that you are open to receiving new evidence, even if that evidence contradicts your current beliefs. You should be open to changing your mind. However, this truism gives rise to theparadox of empathy. The paradox arises with the possibility of mental corruption through transformative change, and has consequences for how we should understand tolerance, disagreement, and the ability to have an open mind. I close with a discussion of how understanding this paradox provides a new explanation for a certain kind of standoff between the believer and the skeptic with regard to religious belief.