bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comparative Transcriptomic Analysis Identifies Key Cellulose Synthase Genes (CESA) and Cellulose Synthase-Like Genes (CSL) in Bast Fiber Development Stage of Flax (Linum Usitatissimum L.)
Authors: Guo Yuan , Wen Lan , Chen Jikang , Pan Gen , Wu Zhimin , Li Zheng , Wang Hui , Xiao Qingmei , Qiu Caisheng , Long Songhua , Zhao Xinlin , Wang Yufu 
Published: April 2021

Abstract
Background: The cellulose synthase gene superfamily, including the cellulose synthase (CESA) and cellulose synthase-like (CSL) gene families, is vital for cell wall construction during plant growth, particularly for fiber development of flax, which is an old and important fiber crop. Results: This study performed a sequencing search of key CESA and CSL genes from several flax stem parts at different fiber development stages by comparing RNA-Seq. Quantitative RT-PCR was used to validate the expression of these genes. This study revealed that CESA4 genes (Lus10008225.g and Lus10008226.g), CESA6 genes (Lus10006161.g and Lus10041063.g), CESA8 genes (Lus10007296.g and Lus10029245.g), CSLD4 gene (Lus10026568.g), CSLE1 (Lus10016625.g) and CSLG genes (Lus10023056.g and Lus10023057.g) were specifically expressed in stem tissue below the snap point where fibers is increased amounts of secondary cell wall deposition. LusCESA4 genes, LusCESA8, genes and LusCSLD4 gene were specifically expressed in fiber development stage during the fast growth period of flax plants. Based on GO and KEGG analyses, it was found that genes involved in pathways of cellulose microfibril organization, galactosyl transferase activity and galactose metabolism were specifically enriched in the stem tissue of the fiber development stage. Other genes involved in cellulose biosynthesis were also analyzed and discussed. Conclusion: The results of this study will provide an important foundation for understanding fiber cell wall biogenesis, particularly the roles of LusCESAs and LusCSLs in the process of fiber development.
Epigenetic DNA changes in childhood acute lymphoblastic leukemia - the drug treatment effect.
Authors: Rozalski Rafal , Gackowski Daniel , Skalska Aleksandra , Starczak Marta , Siomek-Gorecka Agnieszka Siomek-Gorecka , Zarakowska Ewelina , Modrzejewska Martyna , Dziaman Tomasz , Szpila Anna , Linowiecka Kinga , Guz Jolanta , Szpotan Justyna , Gawronski Maciej , Labejszo Anna , Gackowska Lidia , Foksinski Marek , Olinska Elwira , Wasilow Aleksandra , Koltan Andrzej , Styczynski Jan , Olinski Ryszard 
Published: April 2021

Abstract
The active DNA demethylation process may be linked with aberrant methylation and may be involved in leukemogenesis. We investigated the role of epigenetic DNA modifications in childhood acute lymphoblastic leukemia (ALL) development. We analyzed the levels of 5-methyl-2′-deoxycytidine (5-mdC) oxidation products in the cellular DNA and urine of children with ALL (at diagnosis and after completion of chemotherapy, n=55) using two-dimensional ultra-performance liquid chromatography with tandem mass spectrometry (2D UPLC–MS/MS). Moreover, the expression of Ten Eleven Translocation enzymes (TETs) at the mRNA and protein levels was determined. Additionally, the ascorbate level in blood plasma was analyzed. Before treatment, the ALL patients had profoundly higher levels of the analyzed modified DNA in their urine than the controls. After chemotherapy, we observed a statistically significant decrease in active demethylation products in urine, with the final level similar to the level characteristic of healthy children. The level of 5-hmdC on the DNA of the blood leukocytes in the patient group was significantly lower than it was in the control group. Our data suggest that urinary excretion of epigenetic DNA modification may be a marker of pediatric ALL pathogenesis and a reliable marker of chemotherapy response.
Breast Cancer Patients’ Experience and Wishes Regarding Communication on Sexual Health - The BEROSE Study
Authors: Aupomerol Marion , Chaltiel Dan , Pautier Patricia , Wehrer Delphine , Veron Lucie , Degousée Léna , Fasse Leonor , Guéroult-Accolas Laure , Meglio Antonio Di , Scotté Florian , De-Jesus Anne , Vaz-Luis Inès , Delaloge Suzette , Pistilli Barbara 
Published: April 2021

Abstract
Purpose: Although sexual health (SH) is a major concern for many patients with breast cancer (BC), only few of them receive adequate information on this topic throughout their care. BEROSE is a single-center pilot cross-sectional observational study, which aimed at evaluating communication on SH between women with BC and their health-care providers.Methods: From June 12th to July 31th, 2020, a survey was distributed to patients consulting at Breast Cancer Unit of Gustave Roussy Cancer Center. The primary endpoint was the rate of women reporting to have received information on SH from health professionals throughout active treatment administration or BC follow-up.Results: Of 348 surveys collected, 318 were completely filled and included in the analysis. Sixty-one percent of women were older than 50 years and 65% had a localized BC. The majority (63%) was receiving hormone-therapy (37%) or chemotherapy (26%). Overall, 65% reported that they had not received any information about SH throughout their care, while 69% felt it would have been important to discuss sexual issues. Most of women expected professionals to bring up the topic, preferably oncologists or gynecologists, during face-to-face consultations. Concerning satisfaction on SH, 83% reported of being satisfied or very satisfied before BC diagnosis, and only 30% at the time of our survey. Conclusion: Most of women treated or followed-up for BC feel that they lack of information regarding SH-related issues. The impact of BC diagnosis and treatment on SH should be discussed with all women from the first visit and then regularly readdressed.
Synthesis and Characterization of Biodegradable Polyester/Polyether WPU As The Environmental Protection Coating
Authors: Wu Guangfeng , Song Xin , Yang ZhiHui , Li Ying Chun , Zhang HuiXuan 
Published: April 2021

Abstract
Polyester diol PCL and PBA, polyether diol PTMG and polycarbonate diol PCDL were used as components of WPU soft segment, respectively. Polyether PTMG-WPU has the worst hydrolytic property and the highest thermal stability. The maximum degradation rate temperature Tmax is 407.8°C, the water contact angle reaches 89.5°. Traditional polyester PCL-WPU shows the strongest hydrolysis performance, the smallest water contact angle, only 71.7°, the water absorption rate of 72 hours at room temperature is as high as 26.7%. However, the thermal stability of PCL-WPU is lower, the soft segment Tg is -52.3°C, and Tmax is only 333.7°C, but the mechanical propertie of which is the best, the tensile strength is 58.3 MPa, and the elongation at break reaches 857.9%. The most important thing is that the structure of polyester PCL-WPU is more easily destroyed by lipase and water molecules. The acidic products produced after hydrolysis will further promote the degradation of polyester. Therefore, compared with other WPUs, PCL -WPU has the best biodegradability and the most obvious degradation effect under the same conditions. The degradation rate of PTMG-WPU after 30 days of degradation in 0.6% lipase PBS buffer solution and soil was only 4.2% and 2.3%, while the highest degradation rate of traditional polyester PCL-WPU reached 41.7% and 32.0%, respectively. In addition, polycarbonate PCDL-WPU has the highest hardness, reaching 95.5 HD. But its other performances are lower than PCl-WPU.
RGD-Functionalized Melanin Nanoparticles for Intraoperative Photoacoustic Imaging-Guided Breast Cancer Surgery
Authors: Liu Jing-Jing , Wang Zun , Nie Li-Ming , Zhu Yuan-Yuan , Li Ge , Lin Lin-Ling , Chen Min , Zhang Guo-Jun 
Published: April 2021

Abstract
Purpose: Obtaining tumour-free margins is critical for avoiding re-excision and 35 reducing local recurrence following breast-conserving surgery (BCS); however, it 36 remains challenging. Imaging-guided surgery provides precise detection of residual 37 lesions and assists surgical resection. Herein, we describe water-soluble melanin 38 nanoparticles (MNPs) conjugated with cyclic Arg-Gly-Asp (cRGD) peptides for breast 39 cancer photoacoustic imaging (PAI) and surgery navigation. 40Methods: cRGD-MNPs was synthesized and characterized for morphology, 41 photoacoustic characteristics and stability. Tumour targeting and toxicity were 42 determined by cells and tumour-bearing mice. PAI was used to locate the tumour and 43 guide surgical resection in MDA-MB-231 tumour-bearing mice. 44Results: The cRGD-MNPs exhibited excellent tumour-targeting in vitro and in vivo, 45 with low toxicity. Intravenous administration of cRGD-MNPs to MDA-MB-231 46 tumour-bearing mice showed an approximately 2.1-fold enhancement in photoacoustic 47 (PA) intensity at 2 h, and the ratio of the PA intensity at the tumour site compared to 48 that in the surrounding normal tissue was 3.2 ± 0.1, which was much higher than that 49 using MNPs alone (1.7 ± 0.3). Similarly, the PA signal in the mammary glands 50 containing spontaneous breast cancer was enhanced (2.5 ± 0.3-fold) in MMTV-PyVT 51 transgenic murine model. Preoperative screening by PAI could assess tumour volume 52 and offer a three-dimensional (3D) reconstruction image for accurate surgical planning. 53 Surgical resection following real-time PAI on the tumour bed showed high consistency 54 with histopathological analysis. 55Conclusion: These results highlight that cRGD-MNPs combined with PAI provide 56 a powerful tool for breast cancer imaging and precise tumour resection. cRGD-MNPs 57 with good PA properties have great potential for clinical translation.
Synthetic Sphingolipid Analogs and Anti-cancer Drugs Synergistically Induced Human Colon and Pancreatic Cancer Cell Death Through Inhibiting Ceramide Metabolism
Authors: Song Jing , Dagan Arie 
Published: April 2021

Abstract
Ceramide metabolism is a potential target for anti-cancer therapy. Studies show that chemotherapeutic agents can induce apoptosis and it is mediated by ceramide. Synthesized sphingolipid analogs can induce cell death in human lymphocytes and leukemia cells. By screening a group of synthetic sphingolipid analogs, we found that low concentrations of AD2750 and AD2646 induced cell death in human cancer cells by preventing ceramide from converting to sphingomyelin, individually or in combination with commercial cancer drugs. The combination of low concentrations of Taxol and AD2750 or AD2646 significantly increased cell death on human colon cancer cells (HT29). Co-administering low concentrations of Doxorubicin with AD2750 or AD2646 elevated cellular toxicity on human pancreatic cancer cells (CRL1687). This synergistic effect is related to the elevated cellular ceramide. Combining AD2750 or AD2646 with chemotherapy drugs can be used to manipulate ceramide and sphingomyelin metabolism, potentially to affect the growth of human cancer cells and increase the effectiveness of anti-cancer drugs on killing cancer cells.
Early initiation of breastfeeding is protective against child stunting in South Sulawesi: A Cross-Sectional Study
Authors: SIRAJUDDIN SIRAJUDDIN , Sirajuddin Saifuddin , Thaha A Razak , Razak Amran , Thaha Ridwan M , Ansariadi Ansariadi 
Published: April 2021

Abstract
BackgroundSouth Sulawesi in 2018 was declared as the region with the fifth-highest position of stunting children. increasing the risk of excess nutrition and non-communicable diseases.ObjectivesTo investigate The early breastfeeding initiation practices dan stunting children in South Sulawesi Province, Indonesia.MethodsThe surveys were designed by a cross-sectional study. The sample size proportional to children 0–50 months. The household was eligible for inclusion in the survey if they contained a child aged 0–23 month and if the primary caregiver was present for interviews. Sampling technique determined by cluster sampling for each district.ResultsThe distribution of EBI practices in all districts/cities in South Sulawesi is between 14.93% to 90.97 %. EBI status is the majority of EBI 55.2%, duration with the highest percentage of ½ to 1 hour 48.2%. The Suckling process is good at 18.2% while others don't know. Timing is generally < 15 minutes 36.9%. Nutritional status data were known to severely stunting 6.1% and moderately stunting 15.6%.ConclusionEBI practices are more in urban than rural. Early initiation of breastfeeding is protective against child stunting in South Sulawesi
Reconstruction Method of Landscape Planning Mode based on VR Technology and Wireless Communication Technology
Authors: Yao Hang , Jiang Yan , Yang Rongqing 
Published: April 2021

Abstract
With the increasing complexity of human settlements and the deterioration of the ecological environment, landscape design shoulders the arduous responsibility. However, due to various reasons, the digital process of landscape planning and design has lagged behind the times. This paper discusses the difficulties of artificial intelligence reconstruction design in current landscape design, and points out the necessity of promoting artificial intelligence reconstruction design in landscape design. This paper defines the definition, characteristics and concept pedigree of landscape architecture artificial intelligence reconstruction design, analyzes the main digital design methods and application software platform in the concept pedigree, and summarizes the flow chart of landscape architecture artificial intelligence reconstruction design based on VR technology and wireless communication technology. Finally, the limitations and misunderstandings of current AI reconfiguration design are analyzed.
Prognostic value of preoperative inflammatory markers in patients with hepatocellular carcinoma underwent curative resection
Authors: Wu Wenlong , Wang Quancheng , Han Dandan , Li Jianhui , Nie Ye , Guo Dongnan , Yang Long , Tao Kaishan , Zhang Xuan , Dou Kefeng 
Published: April 2021

Abstract
Background: The prognosis of hepatocellular carcinoma (HCC) is not optimistic. Our study focused on present inflammatory markers, including neutrophil-to-lymphocyte ratio (NLR), platelet-to-lymphocyte ratio (PLR), gamma-glutamyl transpeptidase-to-platelet ratio (GPR), aspartate aminotransferase-to-lymphocyte ratio (ALR) and fibrinogen-to-albumin ratio (FAR), and aimed to explore their optimal combination for the prognosis of HCC after resection.Methods: 347 HCC patients with curative resection were enrolled. The optimal cutoff values of the inflammatory markers were calculated using receiver operating characteristic (ROC) curve analysis, and used to divide patients into two groups whose differences were compared by Kaplan-Meier analysis. Cox univariate and multivariate analysis were used to analyze the independent prognostic inflammatory markers. c2 test was chosen to determine the relationship between independent prognostic inflammatory markers and clinicopathological features. We created the combined scoring models and evaluated them by Cox univariate and multivariate methods. The concordance index (C-index), Akaike information criterion (AIC) and likelihood ratio were calculated to compare the models. The selected optimal inflammatory markers and their combinations were tested in different stages of HCC by Kaplan-Meier analysis.Results: ALR and GPR were independent prognostic factors for DFS; ALR, PLR, and GPR were independent prognostic factors for OS. The proposed GPR and ALR-GPR-PLR score models were independent predictors for DFS and OS, respectively.Conclusion: The preoperative GPR and ALR-GPR-PLR score models were independent predictors for DFS and OS, respectively, and performed well in stratifying patients with HCC. The higher score in the model, the worse the prognosis was.
MiR-200c Sensitizes Breast Cancer Cells to Carboplatin Treatment by Decreasing MDR1 Expression
Authors: Najjary Shiva , Safaee Sahar , Mokhtarzadeh Ahad , Amini Mohammad , Bolandi Nadia , Alizadeh Nazila , Hajiasgharzadeh Khalil , Baradaran Behzad 
Published: April 2021

Abstract
Background: Breast cancer (BC) is one of the most common cancers worldwide and is associated with a high rate of cancer mortality in women. Resistance to chemotherapy is considered a significant problem and a major challenge for the treatment of patients with BC. miR-200c belongs to a family of miRNAs that act as tumor inhibitors. The expression level of miR-200c has been reported to be decreased in cancers, especially in BC. The increased miR-200c expression can be considered as a potent inhibitor of drug resistance and tumor progression. Methods and Results: The current study examined the effect of miR-200c on enhancing the BC cells' sensitivity to Carboplatin through targeting MDR1 expression. To perform functional analyses, mimic miR-200c transfected to MCF7 cells. Then, the viability of the cells was investigated via MTT assay. Finally, the expression of associated genes assessed using qRT-PCR. The results indicated that downregulation of miR-200c was occurred in MCF7 cells in comparison to control. Besides, restoring miR-200c expression by regulating the expression level of the apoptotic gene reduces the viability of cancer cells. Moreover, miR-200c increased the sensitivity of MCF7 cells to Carboplatin via reducing the MDR1 gene expression. Conclusions: This study provided valuable data showing that miR-200c enhances the effect of carboplatin as a clinically approved chemotherapeutic agent, and restoring its expression could be considered as a promising targeted adjuvant therapy for BC management.
Complete Genome sequence of a new variant of jujube mosaic-associated virus isolated from jujube (Ziziphus jujuba Mill.) grown at Aksu in Xinjiang of China
Authors: Bai Jianyu , Liu Baojun , Zhang Guoxin , Gu Aixing , Song Danbo , Wang Quan , Li Hong 
Published: April 2021

Abstract
This work reports the discovery and the complete genome sequence of a novel member of the genus Badnavirus in the family Caulimoviridae from a Chinese jujube tree of known variety which grown in Aksu, Xinjiang, China. The symptoms of jujube leaves infected by the virus showed mosaic and malformation, and round chlorotic spots on infected fruits. The genome of this virus is a circular double-stranded DNA, which has the length of 7086 nt and has a genome structure similar to the one reported for jujube mosaic-associated virus (JuMaV), with five open reading frames (ORFs). High nucleotide and amino acid sequence similarity were seen between JuMaV and the new virus, and the nucleotide (NT) difference of the genome was greater than 20% in the RT/ Rnase H coding region of ORF3. Consequently, the virus was identified a new variant of JuMaV, and propose the name as jujube mosaic-associated virus A (JuMaVA).
SARS-COV-2 Colonizes Coronary Thrombus and Impairs Heart Microcirculation Bed in Asymptomatic Positive Subjects with Acute Myocardial Infarction
Authors: marfella raffaele , Paolisso Pasquale , Sardu Celestino , Palomba Luciana , D'Onofrio Nunzia , Cesaro Arturo , Barbieri Michelangela , Rizzo Maria Rosaria , Sasso Ferdinando Carlo , Scisciola Lucia , Turriziani Fabrizio , Galdiero Massimiliano , Pignataro Danilo , Minicucci Fabio , Trotta Maria Consiglia , D'Amico Michele , Mauro Ciro , Calabrò Paolo , Balestrieri Maria Luisa , Barbato Emanuele , Galdiero Marilena , Paolisso Giuseppe 
Published: April 2021

Abstract
Background. The viral load of asymptomatic SAR-COV-2 positive (ASAP) persons have been equal to that of symptomatic patients, suggesting a similar risk for endothelial dysfunction and increased coagulation in asymptomatic and symptomatic patients. To date, there are no reports of ST-elevation myocardial infarction (STEMI) outcomes in ASAP patients. We evaluated thrombus burden and thrombus viral load and their impact on microvascular bed perfusion in the infarct area (myocardial lush grade, MBG) in ASAP compared to SARS-COV-2 negative (SANE) STEMI patients. Methods. This was an observational study of 46 ASAP, and 130 SANE patients admitted with confirmed STEMI treated with primary percutaneous coronary intervention and thrombus aspiration. The primary endpoints were thrombus dimension + thrombus viral load effects on MBG after PPCI. The secondary endpoints during hospitalization were major adverse cardiovascular events (MACEs). MACEs are defined as a composite of cardiovascular death, nonfatal acute AMI, and heart failure during hospitalization.Results. Thrombus dimensions were significantly higher in ASAP patients as compared to SANE patients. Interestingly, 39 (84.9%) of ASAP patients also had thrombus specimens positive for SARS-COV-2. In ASAP STEMI patients (n=46), thrombus viral load was a significant determinant of thrombus dimension independently of risk factors (p<0.005). MBG and left ventricular function were significantly lower in ASAP STEMI patients (p<0.001). Multiple logistic regression analyses evidenced that thrombus SARS-CoV-2 infection and dimension were significant predictors of poorer MBG in STEMI patients. Conclusions. In ASAP patients presenting with STEMI, there is strong evidence towards higher thrombus viral load, dimension, and poorer MBG. These data support the need to reconsider ASAP status as a risk factor that may worsen STEMI outcomes.
Weekly Bayesian Modelling Strategy to Predict Deaths by COVID-19: a Model and Case Study for the State of Santa Catarina, Brazil
Authors: Avelar Pedro Henrique , Lamb Luis , Tsoka Sophia , Cardoso-Silva Jonathan 
Published: April 2021

Abstract
Background: The novel coronavirus pandemic has affected Brazil's Santa Catarina State (SC) severely. At the time of writing (24 March 2021), over 764,000 cases and over 9,800 deaths by COVID-19 have been confirmed, hospitals were fully occupied with local news reporting at least 397 people in the waiting list for an ICU bed. Despite initial state-wide measures at the outbreak of the pandemic, the state government passed most responsibilities down to cities local government, leaving them to plan whether and when to apply Non-Pharmaceutical Interventions (NPIs). In an attempt to better inform local policy making, we applied an existing Bayesian algorithm to model the spread of the pandemic in the seven geographic macro-regions of the state. However, as we found that the model was too reactive to change in data trends, here we propose changes to extend the model and improve its forecasting capabilities. Methods: Our four proposed variations of the original method allow accessing data of daily reported infections and take into account under-reporting of cases more explicitly. Two of the proposed versions also attempt to model the delay in test reporting. We simulated weekly forecasting of deaths from the period from 31/05/2020 until 31/01/2021.First week data were used as a cold-start to the algorithm, after which weekly calibrations of the model were able to converge in fewer iterations. Google Mobility data were used as covariates to the model, as well as to estimate of the susceptible population at each simulated run. Findings: The changes made the model significantly less reactive and more rapid in adapting to scenarios after a peak in deaths is observed. Assuming that the cases are under-reported greatly benefited the model in its stability, and modelling retroactively-added data (due to the “hot” nature of the data used) had a negligible impact in performance. Interpretation: Although not as reliable as death statistics, case statistics, when modelled in conjunction with an overestimate parameter, provide a good alternative for improving the forecasting of models, especially in long-range predictions and after the peak of an infection wave.