bluuebunny/crossref_metadata_2025_split huggingface dataset:
Calorimetric analysis of ice onset temperature during cryoablation: a model approach to identify early predictors of effective applications
Authors: Campagnoli Elena , Ballatore Andrea , Giaretto Valter , Anselmino Matteo 
Published: August 2021

AbstractAim of the present study is to analyze thermal events occurring during cryoablation. Different bovine liver samples underwent freezing cycles at different cooling rate (from 0.0075 to 25 K/min). Ice onset temperature and specific latent heat capacity of the ice formation process were measured according to differential scanning calorimetry signals. A computational model of the thermal events occurring during cryoablation was compiled using Neumann’s analytical solution. Latent heat (#1 = 139.8 ± 7.4 J/g, #2 = 147.8 ± 7.9 J/g, #3 = 159.0 ± 4.1 J/g) of all liver samples was independent of the ice onset temperature, but linearly dependent on the water content. Ice onset temperature was proportional to the logarithm of the cooling rate in the range 5 ÷ 25 K/min (#3a = − 12.2 °C, #3b = − 16.2 °C, #3c = − 6.6 °C at 5K/min; #3a = − 16.5 °C, #3b = − 19.3 °C, #3c = − 11.6 °C at 25 K/min). Ice onset temperature was associated with both the way in which the heat involved into the phase transition was delivered and with the thermal gradient inside the tissue. Ice onset temperature should be evaluated in the early phase of the ablation to tailor cryoenergy delivery. In order to obtain low ice trigger temperatures and consequent low ablation temperatures a high cooling rate is necessary.
A generic intelligent tomato classification system for practical applications using DenseNet-201 with transfer learning
Authors: Lu Tao , Han Baokun , Chen Lipin , Yu Fanqianhui , Xue Changhu 
Published: August 2021

AbstractA generic intelligent tomato classification system based on DenseNet-201 with transfer learning was proposed and the augmented training sets obtained by data augmentation methods were employed to train the model. The trained model achieved high classification accuracy on the images of different quality, even those containing high levels of noise. Also, the trained model could accurately and efficiently identify and classify a single tomato image with only 29 ms, indicating that the proposed model has great potential value in real-world applications. The feature visualization of the trained models shows their understanding of tomato images, i.e., the learned common and high-level features. The strongest activations of the trained models show that the correct or incorrect target recognition areas by a model during the classification process will affect its final classification accuracy. Based on this, the results obtained in this study could provide guidance and new ideas to improve the development of intelligent agriculture.
Quantifying the pattern of retinal vascular orientation in diabetic retinopathy using optical coherence tomography angiography
Authors: Ma Yanhui , Ohr Matthew P. , Pan Xueliang , Roberts Cynthia J. 
Published: August 2021

AbstractQuantitative imaging using optical coherence tomography angiography (OCTA) could provide objective tools for the detection and characterization of diabetic retinopathy (DR). In this study, an operator combining the second derivative and Gaussian multiscale convolution is applied to identify the retinal orientation at each pixel in the OCTA image. We quantified the pattern of retinal vascular orientation and developed three novel quantitative metrics including vessel preferred orientation, vessel anisotropy, and vessel area. Each of eight 45º sectors of the circular disk centered at the macular region was defined as the region of interest. Significant sectoral differences were observed in the preferred orientation (p < 0.0001) and vessel area (p < 0.0001) in the 34 healthy subjects, whereas vessel anisotropy did not demonstrate a significant difference among the eight sectors (p = 0.054). Differential retinal microvascular orientation patterns were observed between healthy controls (n = 34) and the DR subjects (n = 7). The vessel area characterized from the vascular orientation pattern was shown to be strongly correlated with the traditionally reported vessel density (Pearson R > 0.97, p < 0.0001). With three metrics calculated from the vascular orientation pattern simultaneously and sectorally, our quantitative assessment for retinal microvasculature provides more information than vessel density alone and thereby may enhance the detection of DR. These preliminary results suggest the feasibility and advantage of our vessel orientation-based quantitative approach using OCTA to characterize DR-associated changes in retinal microvasculature.
Scale-up approach for supercritical fluid extraction with ethanol–water modified carbon dioxide on Phyllanthus niruri for safe enriched herbal extracts
Authors: Hassim Norsyamimi , Markom Masturah , Rosli Masli Irwan , Harun Shuhaida 
Published: August 2021

AbstractScaling-up supercritical fluid extraction (SFE) for the extraction of bioactive compounds from herbal plants is challenging, especially with the presence of alcohol-water as co-solvent. Hence, the main objective of this study is to validate the scale-up criteria of SFE process forPhyllanthus niruri(P. niruri), and analyse the extract safety and profitability process at the industrial scale. The study was performed by using supercritical carbon dioxide (SC-CO2) with ethanol–water co-solvent at two operating conditions (L1: 200 bar, 60 °C and L2: 262 bar, 80 °C). The solvent-to-feed ratio (S/F) scale-up validation experiments were conducted at both operating conditions with feed mass capacity of 0.5 kg. The extraction yields and overall extraction curves obtained were almost similar to the predicted ones, with error of 5.13% and 14.2%, respectively. The safety of scale-up extract was evaluated by using a toxicity test against zebrafish embryo (FETT). The extract exhibited a low toxic effect with the LD50value of 505.71 µg/mL. The economic evaluation using a detailed profitability analysis showed that the SFE ofP. niruriwas an economically feasible process, as it disclosed the encouraging values of return on investment (ROI) and net present values (NPV) for all scale-up capacities.
Acetylation turns leucine into a drug by membrane transporter switching
Authors: Churchill Grant C. , Strupp Michael , Factor Cailley , Bremova-Ertl Tatiana , Factor Mallory , Patterson Marc C. , Platt Frances M. , Galione Antony 
Published: August 2021

AbstractSmall changes to molecules can have profound effects on their pharmacological activity as exemplified by the addition of the two-carbon acetyl group to make drugs more effective by enhancing their pharmacokinetic or pharmacodynamic properties.N-acetyl-d,l-leucine is approved in France for vertigo and itsl-enantiomer is being developed as a drug for rare and common neurological disorders. However, the precise mechanistic details of how acetylation converts leucine into a drug are unknown. Here we show that acetylation of leucine switches its uptake into cells from thel-type amino acid transporter (LAT1) used by leucine to organic anion transporters (OAT1 and OAT3) and the monocarboxylate transporter type 1 (MCT1). Both the kinetics of MCT1 (lower affinity compared to LAT1) and the ubiquitous tissue expression of MCT1 make it well suited for uptake and distribution ofN-acetyl-l-leucine. MCT1-mediated uptake of aN-acetyl-l-leucine as a prodrug of leucine bypasses LAT1, the rate-limiting step in activation of leucine-mediated signalling and metabolic process inside cells such as mTOR. Converting an amino acid into an anion through acetylation reveals a way for the rational design of drugs to target anion transporters.
Biosynthesis of silver nanoparticles with antimicrobial and anticancer properties using two novel yeasts
Authors: Liu Xin , Chen Jia-Le , Yang Wen-Yu , Qian Yu-Cheng , Pan Jing-Yu , Zhu Chen-Nianci , Liu Li , Ou Wen-Bin , Zhao Hong-Xin , Zhang Dian-Peng 
Published: August 2021

AbstractAgNPs are nanomaterials with many potential biomedical applications. In this study, the two novel yeast strains HX-YS and LPP-12Y capable of producing biological silver nanoparticles were isolated. Sequencing of ribosomal DNA-ITS fragments, as well as partial D1/D2 regions of 26S rDNA indicated that the strains are related to species from the genus Metschnikowia. The BioAgNPs produced by HX-YS and LPP-12Y at pH 5.0–6.0 and 26 °C ranged in size from 50 to 500 nm. The antibacterial activities of yeast BioAgNPs against five pathogenic bacteria were determined. The highest antibacterial effect was observed on P. aeruginosa, with additional obvious effects on E. coli ATCC8099 and S. aureus ATCC10231. Additionally, the BioAgNPs showed antiproliferative effects on lung cancer cell lines H1975 and A579, with low toxicity in Beas 2B normal lung cells. Therefore, the AgNPs biosynthesized by HX-YS and LPP-12Y may have potential applications in the treatment of bacterial infections and cancer.
Peer pressure induced punishment resolves social dilemma on interdependent networks
Authors: Hu Kaipeng , Tao Yewei , Ma Yongjuan , Shi Lei 
Published: August 2021

AbstractDespite the fruitful evidence to support the emergence of cooperation, irrational decisions are still an essential part of promoting cooperation. Among the many factors that affect human rational decision-making, peer pressure is unique to social organisms and directly affects individual cooperative behaviors in the process of social interaction. This kind of pressure psychologically forces individuals to behave consistently with their partners, and partners with inconsistent behaviors may suffer psychological blows. As feedback, this psychological harm may in turn affect individual cooperative decisions. There is evidence that when peer pressure exists, partnerships can reduce free-riding in enterprise. Based on interdependent networks, this paper studies the impact of peer pressure on cooperation dynamics when the strategies of corresponding partners from different layers of the networks are inconsistent. We assume that when individuals are under peer pressure, their payoffs will be compromised. The simulation results show that the punishment effect will force the expulsion of partners with different strategies, which will further reduce the proportion of partners with inconsistent strategies in the system. However, in most cases, only moderate fines are most conductive to the evolution of cooperation, and the punishment mechanisms can effectively promote the interdependent network reciprocity. The results on the small world and random network prove the robustness of the result. In addition, under this mechanism, the greater the payoff dependence between partners, the better the effect of interdependent network reciprocity.
Novel insights into peptide amidation and amidating activity in the human circulation
Authors: Kaufmann Paul , Bergmann Andreas , Melander Olle 
Published: August 2021

AbstractC-terminal α-amidation is the final and essential step in the biosynthesis of several peptide hormones. Peptidylglycine α-amidating monooxygenase (PAM) is the only known enzyme to catalyse this reaction. PAM amidating activity (AMA) is known to be present in human circulation, but its physiological role and significance as a clinical biomarker remains unclear. We developed a PAM-specific amidation assay that utilizes the naturally occurring substrate Adrenomedullin-Gly (ADM-Gly, 1–53). Using our amidation assay we quantified serum amidating activities in a large population-based cohort of more than 4900 individuals. A correlation of serum amidating activity with several clinical parameters including high blood pressure was observed. Increasing PAM-AMA was an independent predictor of hard outcomes related to hemodynamic stress such as cardiovascular mortality, atrial fibrillation and heart failure during long-term follow-up (8.8 ± 2.5 years). Moreover, results from an animal study in rats utilizing recombinant human PAM provide novel insights into the physiological role of circulating PAM and show its potential significance in circulating peptide amidation.
Effects of diabetes mellitus on the rate of carpal tunnel release in patients with carpal tunnel syndrome
Authors: Shin Jaeyong , Kim Yong Wook , Lee Sang Chul , Yang Seung Nam , Chang Jee Suk , Yoon Seo Yeon 
Published: August 2021

AbstractThe objective of this study was to evaluate the effects of diabetes mellitus (DM) on the rate of carpal tunnel release (CTR) using a large nationwide cohort in Korea and to identify risk factors, including comorbidities and socioeconomic status (SES), associated with CTR. Patients with a primary or secondary diagnosis of carpal tunnel syndrome (CTS; ICD-10 code: G560) were selected and divided into two groups according to the presence of DM. A Cox proportional hazard model was used to assess the rate of CTR between the two groups. To evaluate the influence of demographic factors, comorbidities, and SES on CTR, multivariate Cox proportional hazard regression models were used to adjust for confounding variables. In total, 12,419 patients with CTS were included in the study: 2487 in DM cohort and 9932 in non-DM cohort. DM duration was negatively related with the rate of CTR (HR = 0.89, 95% CI 0.87–0.91) in CTS patients with DM. The rate of CTR was decreased in patients with DM compared to those without DM in the unadjusted model; however, after adjusting for comorbidities, DM had no significant effect on the rate of CTR. Female sex (HR = 1.50, 95% CI 1.36–1.67) correlated with the rate of CTR, and an inverse relationship between the number of comorbidities and CTR was found (p < 0.001) irrespective of DM. Diabetic polyneuropathy (DPN) was not associated with CTR, and we did not find any factors correlating with CTR in DPN patients. We found that CTS patients with more comorbidities or combined with a longer duration of DM were undertreated in real-word practice. Actual outcomes of CTR in CTS patents with various comorbidities should be investigated in future studies for optimal management of CTS.
Eristalis flower flies can be mechanical vectors of the common trypanosome bee parasite, Crithidia bombi
Authors: Davis Abby E. , Deutsch Kaitlin R. , Torres Alondra M. , Mata Loya Mesly J. , Cody Lauren V. , Harte Emma , Sossa David , Muñiz Paige A. , Ng Wee Hao , McArt Scott H. 
Published: August 2021

AbstractFlowers can be transmission platforms for parasites that impact bee health, yet bees share floral resources with other pollinator taxa, such as flies, that may be hosts or non-host vectors (i.e., mechanical vectors) of parasites. Here, we assessed whether the fecal-orally transmitted gut parasite of bees,Crithidia bombi, can infectEristalis tenaxflower flies. We also investigated the potential for two confirmed solitary bee hosts ofC. bombi,Osmia lignariaandMegachile rotundata, as well as two flower fly species,Eristalis arbustorumandE. tenax,to transmit the parasite at flowers. We found thatC. bombidid not replicate (i.e., cause an active infection) inE. tenaxflies. However, 93% of inoculated flies defecated liveC. bombiin their first fecal event, and all contaminated fecal events containedC. bombiat concentrations sufficient to infect bumble bees. Flies and bees defecated inside the corolla (flower) more frequently than other plant locations, and flies defecated at volumes comparable to or greater than bees. Our results demonstrate thatEristalisflower flies are not hosts ofC. bombi, but they may be mechanical vectors of this parasite at flowers. Thus, flower flies may amplify or diluteC. bombiin bee communities, though current theoretical work suggests that unless present in large populations, the effects of mechanical vectors will be smaller than hosts.
Responses of maize hybrids with contrasting maturity to planting date in Northeast China
Authors: Ke Fulai , Ma Xinglin 
Published: August 2021

AbstractMaize sowing in semi-humid region of Jilin province is often delayed beyond the optimum planting time window because of soil water stress typically occurring before or during the planting season. Research was conducted at Lishu city, in Jilin province from 2009 through 2010 to determine the responses of maize hybrids with contrasting maturity to planting date. Three popular hybrids with contrasting different maturity, short-season hybrid Jidan27, mid-season hybrid Xianyu335, and full-season hybrid Zhengdan958 were planted in early May and mid-May and thinned to populations of 67,500 plants ha−1. The results showed that durations from emergence to silking stage for all the hybrids consistently shortened as the planting delayed, but interaction effects of hybrids, planting date and year existed for the duration of silking to physiological maturity stage. The longer maturity hybrid usually had grain yield advantage over earlier maturity hybrid when planting at early May, but the earlier maturity hybrid often showed a greater yield than longer maturity hybrid when planting was delayed. The highest yield occurred at the treatment combination of the mid-season hybrid and delayed planting date, and the shorter season hybrid typically showed stable and higher grain yield across planting dates. Changes in grain volume per unit of land area among hybrids and planting date treatment combination were consistent with the changes in grain yields, indicating that the yield is determined usually by the sink capacity. Further research is needed to evaluate the effects of hybrids maturity and planting date on maize under different planting densities.
Response of salivary biomarkers to an empathy triggering film sequence—a pilot study
Authors: Zenzmaier Christoph , Janssen Jessie , Zulmin Christoph , Österreicher Philipp , Heinrich Lea , Tucek Gerhard , Perkhofer Susanne 
Published: August 2021

AbstractEmpathy is a multifaceted phenomenon that is difficult to measure. Self-report questionnaires are the most common and well-validated measures while currently no validated protein biomarkers associated with the empathic reaction have been established. Trigger films have been previously used in psychological research to evoke emotions. Thus, in the present randomized cross-over study we investigated the responses of nine salivary biomarkers that have been related to emotions and stress following an empathy triggering and a control film sequence. Additionally, questionnaires for empathy (Saarbrucken Personality Questionnaire (SPQ)) and current mental stress were applied and participants were asked to assess the film protagonists’ emotions using the Positive and Negative Affect Schedule. Data from 46 participants were included in the analysis. α-Amylase, IgA, IL-1β and estradiol showed a significantly different response between the empathy and control intervention. Moreover, normalized levels of these biomarkers significantly correlated with single scales of the SPQ (control film sequence: α-amylase and IgA with personal distress; estradiol with empathic concern; IL-1β with fantasy; empathy triggering film sequence: IgA with empathic concern, fantasy and the total empathy score). These findings indicated that the observed changes in salivary biomarker levels were reflective of a physiological response to the empathy triggering film sequence. Future studies using different triggers and settings will show if the identified biomarkers can be considered as surrogate markers for empathic reactions in general.
Improve thermostability of Bacillus sp. TS chitosanase through structure-based alignment
Authors: Zhou Zhanping , Wang Xiao 
Published: August 2021

AbstractChitosanases can catalyze the release of chitooligosaccharides which have a number of medical applications. Therefore, Chitosanases are good candidates for large-scale enzymatic synthesis due to their favorable thermostability properties and high catalytic efficiency. To further improve the thermostability of a chitosanase from Bacillus sp. TS, which has a half-life of 5.32 min, we mutated specific serine residues that we identified as potentially relevant through structure comparison with thermophilic CelA from Clostridium thermocellum. Out of a total of 15 mutants, three, namely S265G, S276A, and S347G, show higher thermostability. Their half-lives at 60 °C were calculated as 34.57 min, 36.79 min and 7.2 min. The Km values of S265G, S276A and S347G mutants show substrate binding ability comparable to that of the wild-type enzyme, while the S265G mutant displays a significant decrease of enzymatic activities. Additionally, we studied the synergistic effects of combined mutations, observing that all double mutants and the triple mutant are more stable than the wild-type enzyme and single mutants. Finally, we investigated the mechanisms which might give a reasonable explanation for the improved thermostability via comparative analysis of the resulting 3D structures.
Performance and host association of spotted lanternfly (Lycorma delicatula) among common woody ornamentals
Authors: Uyi Osariyekemwen , Keller Joseph A. , Swackhamer Emelie , Hoover Kelli 
Published: August 2021

AbstractLycorma delicatula (spotted lanternfly) has a broad host range with a strong preference for the invasive host plant from its native range, tree of heaven (Ailanthus altissima); it had long been speculated that L. delicatula could not develop or reproduce without access to tree of heaven. In 2019, we found that this assumption was incorrect, but fitness was reduced in the absence of A. altissima in that the number of egg masses laid was dramatically fewer for insects reared on suitable non-A. altissima host plants that had recently been established. We hypothesized that longer established, larger trees (of the same species) would improve the fitness of L. delicatula in the absence of tree of heaven. In spring 2020, we examined insect performance with and without access to A. altissima by tracking development, survival, host tree association and oviposition in large enclosures with trees planted two years prior to the study. Each enclosure included one each of Juglans nigra, Salix babylonica and Acer saccharinum along with either one A. altissima or one Betula nigra; these trees had twice the diameter of the same trees the previous year. We reared nymphs with and without access to A. altissima, released them into the corresponding large enclosures as third instars, and monitored them from early July 2020 through November 2020. We also determined whether lack of access to A. altissima by parents of L. delicatula have any fitness effects on offspring performance. To ensure adequate adult populations for comparing fecundity between treatments, third instars were released into the multi-tree enclosures due to high mortality in earlier instars that occurred in a similar study in 2019. Insect survival was higher and development faster with access to A. altissima. Third and fourth instar nymphs were most frequently observed on A. altissima when it was present, while adults were equally associated with A. saccharinum and A. altissima. In the absence of A. altissima, nymphs were most frequently found on S. babylonica, while adults were most often on A. saccharinum. Females with access to A. altissima deposited nearly 7-fold more egg masses than those without access to A. altissima, which is consistent with the difference in egg mass numbers between the two treatments the previous year; thus, our hypothesis was rejected. The offspring of parents that had been reared without access to A. altissima showed similar survival and development time from egg to adult as offspring from parents that never had access to A. altissima. These findings suggest that managers need to be aware that even in the absence of A. altissima in the landscape, several hardwood host trees can be utilized by L. delicatula to develop and reproduce, but fitness without A. altissima is likely to still be reduced.