bluuebunny/crossref_metadata_2025_split huggingface dataset:
County-level Analysis of Perinatal Health Indicators Within a Single Health System Catchment
Authors: Lemas Dominick J. , Layton Claire , Ballard Hailey , Xu Ke , Smulian John C. , Gurka Matthew , Loop Matthew Shane , Smith Erica L. , Reeder Callie F. , Louis-Jacques Adetola , Hsiao Chu J , Cacho Nicole , Hall Jaclyn 
Published: December 2021

Abstract
Background: Adverse perinatal health outcomes are disproportionally impacted in rural communities. Social determinants of health (SDoH) defined by nonclinical social, behavioral, and economic factors may impact up to 90% of health outcomes in rural communities. Objective: To evaluate county-level perinatal patterns in health outcomes, health behaviors, socioeconomic vulnerability, and healthcare providers across rural and non-rural Florida counties within a single health system catchment. Methods: Socioeconomic vulnerability metrics, digital connectivity, licensed provider metrics, and behavioral data and were obtained from Floridahealthcharts.com and the County Health Rankings. County-level birth and perinatal health outcome data were obtained from the Florida Department of Health. The University of Florida Health Perinatal Catchment Area (UFHPCA) was defined as all Florida counties where ≥5% of all infants were delivered at Shands Hospital in Alachua county between June 2011 and April 2017. County-level rurality was determined by Florida Statutes 288.0656 rurality designations. Results: The UFHPCA included three non-rural and ten rural counties that represented more than 64,000 deliveries over a 5-year 9-month period. We found that nearly 1 in 3 infants resided in a rural county (n=20,899), and 7 out of 13 counties did not have a licensed obstetrician gynecologist. Nine counties reported maternal death rates that were between 1 and 4-fold higher than the statewide rate, and rural counties generally reported neonatal mortality and preterm birth rates that were higher than the statewide averages. We found maternal smoking rates (range 6.8% – 24.8%) were above the statewide rate (6.2%) for all counties in the catchment. Except for Alachua county, breastfeeding initiation rates (range 54.9% - 81.4%) and access to household computing devices (range 72.8% - 86.4%) were below the statewide rate (82.9% and 87.9%, respectively). Finally, we found that childhood poverty rates (range 16.3% - 36.9%) in our catchment was above the statewide rate (18.5%), except for Suwanee and Columbia counties.Conclusions: The health burden of the UFHPCA is characterized by both rural and non-rural counties with increased maternal and neonatal death and preterm birth, as well as adverse health behaviors that include smoking during pregnancy and lower levels of breastfeeding.
TaxaTarget: Fast, Sensitive, and Precise Classification of Microeukaryotes in Metagenomic Data
Authors: Commichaux Seth , Javkar Kiran , Muralidharan Harihara Subrahmaniam , Ramachandran Padmini , Ottesen Andrea , Rand Hugh , Pop Mihai 
Published: December 2021

Abstract
BackgroundMicrobial eukaryotes are nearly ubiquitous in microbiomes on Earth and contribute to many integral ecological functions. Metagenomics is a proven tool for studying the microbial diversity, functions, and ecology of microbiomes, but has been underutilized for microeukaryotes due to the computational challenges they present. For taxonomic classification, the use of a eukaryotic marker gene database can improve the computational efficiency, precision and sensitivity. However, state-of-the-art tools which use marker gene databases implement universal thresholds for classification rather than dynamically learning the thresholds from the database structure, impacting the accuracy of the classification process.ResultsHere we introduce taxaTarget, a method for the taxonomic classification of microeukaryotes in metagenomic data. Using a database of eukaryotic marker genes and a supervised learning approach for training, we learned the discriminatory power and classification thresholds for each 20 amino acid region of each marker gene in our database. This approach provided improved sensitivity and precision compared to other state-of-the-art approaches, with rapid runtimes and low memory usage. Additionally, taxaTarget was better able to detect the presence of multiple closely related species as well as species with no representative sequences in the database. One of the greatest challenges faced during the development of taxaTarget was the general sparsity of available sequences for microeukaryotes. Several algorithms were implemented, including threshold padding, which effectively handled the missing training data and reduced classification errors. Using taxaTarget on metagenomes from human fecal microbiomes, a broader range of genera were detected, including multiple parasites that the other tested tools missed.ConclusionData-driven methods for learning classification thresholds from the structure of an input database can provide granular information about the discriminatory power of the sequences and improve the sensitivity and precision of classification. These methods will help facilitate a more comprehensive analysis of metagenomic data and expand our knowledge about the diverse eukaryotes in microbial communities.
The Effects of Microglia on Tauopathy Progression Can Be Quantified Using Nexopathy in Silico (Nexis) Models
Authors: Anand Chaitali , Maia Pedro , Torok Justin , Mezias Christopher , Raj Ashish 
Published: December 2021

Abstract
The prion-like transsynaptic propagation of misfolded tau along the brain’s connectome has previously been modeled using connectome-based network diffusion models. In addition to the connectome, interactions between the general neurological ‘milieu’ in the neurodegenerative brain and proteinopathic species can also contribute to pathology propagation. Such a molecular nexopathy framework posits that the distinct characteristics of neurodegenerative disorders stem from interactions between the network and surrounding molecular players. However, the effects of these modulators remain unquantified. Here, we present Nexopathy in silico (‘Nexis’), a quantitative model of tau progression augmenting earlier models by including parameters of pathology propagation defined by the molecular modulators of connectome-based spread. Our Nexis:microglia model provides the first quantitative characterization of this effect on the whole brain by expanding previous models of neuropathology progression by incorporating microglial influence. We show that Trem2, but not microglial homeostasis genes, significantly improved the model’s predictive power. Trem2 appears to reduce tau accumulation rate while increasing its interregional spread from the hippocampal seed area, causing higher tau burden in the striatum, pallidum, and contralateral hippocampus. Nexis thus provides an improved understanding and quantification of microglial contribution to tau propagation and can be flexibly modified to include other modulators of progressive neurodegeneration.
Sleep Quality and Associated Factors Among Type 2 Dm Patients and Non-Dm Individuals in Bahir Dar Governmental Hospitals: Comparative Cross-Sectional Study
Authors: Zewdu Dagmawit , Gedamu Haileyesus , Beyene Yeshiwork , Tadesse Mekdes , Tamirat Mahlet , Muluken Silenat 
Published: December 2021

Abstract
Background: Multiple factors may contribute to sleep disruption in type 2 diabetic individuals. Sleep disruption in type 2 diabetic individuals is frequently associated with long-term damage, dysfunction, and failure of different organs. Nevertheless, literature in this regard is scanty in Ethiopia. Therefore, this study aimed to assess and compare the prevalence of poor sleep quality and associated factors among type 2 diabetic and non-diabetic individuals in Bahir Dar governmental hospitals.Methods: Comparative cross-sectional study was employed among 292 individuals with type 2 diabetes and 291 non-diabetic individuals in Bahir Dar governmental hospitals from March 01- to April-01. A two-stage cluster sampling method was employed to select participants. Pittsburgh sleep quality index was used for assessing sleep quality. For analysis, descriptive and summary statistics were used to determine the prevalence and percentage of variables. Chi-square test was also used for comparison. Binary logistic regression analysis was employed to determine the associated factors of poor sleep quality. Result: The prevalence of poor sleep was 50.7% (95% CI; 44.9-56.2) and 31.8% (95% CI 26.5-37.5) among individuals with type 2 diabetes and non-diabetic individuals respectively. Among the overall participants being type 2 diabetic patient was also found significantly associated with poor sleep quality as compared to non-diabetic individuals (AOR=1.89; 95% CI; 1.19-2.87). Comorbidity, duration of DM >10 years, Poor glycaemic control, having depression, low physical activity, and poor social support were factors significantly associated with poor sleep quality among individuals with type 2 diabetes. Among non-diabetic individual’s low physical activity, poor social support, having depression, and age group (>50 years) were factors significantly associated with poor sleep quality. Conclusion: In this study, the prevalence of poor sleep among individuals with type 2 diabetes was higher than non-diabetes individuals.
Self-Compassion And Imagery Rescripting: A Brief Online Intervention To Reduce Body Dissatisfaction in Women
Authors: Butler Ashley , Talbot Daniel , Smith Evelyn 
Published: December 2021

Abstract
Background: Due to the prevalence of body dissatisfaction, and given that it is a significant risk factor for eating disorders and obesity, understanding the mechanisms of change, and finding new ways on how to reduce body dissatisfaction are important avenues of research. The current study aimed to investigate whether a brief online intervention of imagery rescripting is more effective in reducing state body dissatisfaction in women than the widely used intervention of self-compassion, or a control condition. Methods: One-hundred and twenty-three Australian women with high levels of body dissatisfaction were allocated to one of the three intervention conditions: imagery rescripting, self-compassion, or control. Participants completed a screening questionnaire, a visual analogue scale (VAS) on mood, and a physical appearance state and trait anxiety scale (PASTAS) on state body dissatisfaction before and after a body dissatisfaction inducing mirror task, and again after the intervention. Results: Results showcased a significant decrease in state body dissatisfaction from post-induction to post-intervention for imagery rescripting compared to the control. Conclusion: Imagery rescripting was identified as an effective brief intervention for reducing state body dissatisfaction in women and inspiring body acceptance compared to the control condition. Future studies should investigate the impact of multiple sessions of imagery rescripting for women with high levels of body dissatisfaction.
Human CSF Movement Influenced by Vascular Low Frequency Oscillations and Respiration
Authors: Nair Vidhya Vijayakrishnan , Kish Brianna R , Inglis Ben , Yang Ho-Ching (Shawn) , Wu Yu-Chien , Zhou Xiaopeng , Schwichtenberg Amy J. , Tong Yunjie 
Published: December 2021

Abstract
Background: Cerebrospinal fluid movement (CSF) through the pathways within the central nervous system is of high significance for maintaining normal brain health and function. Low frequency hemodynamics and respiration have both been shown to independently drive CSF in humans. Here, we hypothesize that CSF movement may be driven simultaneously (and in synchrony) by both mechanisms and we study their independent and interactive effects on CSF movement using novel neck fMRI scans.Methods: Caudad CSF movement at the fourth ventricle and hemodynamics of the major neck blood vessels (internal carotid arteries and internal jugular veins) were captured from 11 young healthy volunteers using novel neck fMRI scans with simultaneous measurement of respiration. Two distinct models of CSF movement (1. Low-frequency hemodynamics and 2. Respiration) were independently investigated in corresponding frequency ranges. Possible interactions between these mechanisms were also studied using cross-frequency coupling.Results: The results from this study validated that the caudad CSF movement may be driven by both low frequency hemodynamics (0.01 Hz – 0.1 Hz) and respiration (0.2 Hz - 0.4 Hz), through different mechanisms. We show that the dynamics of brain fluids can be assessed from the neck, by studying the interrelationships between major neck blood vessels and the CSF movement at the fourth ventricle. We also demonstrate that there exists a cross-frequency interaction between two separable mechanisms.Conclusions: The human CSF system is capable of responding to multiple interacting physiological forces at the same time. This information may help inform the pathological mechanisms behind CSF movement-related disorders and facilitate new approaches to therapeutic interventions.
The Research on Stability of Surrounding Rock in Gob-side Entry Driving in Deep and Thick Seam
Authors: SHI jianjun , Jicheng Feng , Rui Peng , Quanjie Zhu 
Published: December 2021

Abstract
The gob-side entry driving is driving in low pressure area, which bears less support pressure and is easy to maintain, so it is widely used. Taking the gob-side entry driving in thick coal seam of Dongtan Coal Mine as an example, the reasonable size of pillar and the section of roadway are numerically simulated by combining numerical with measurement, and the roadway support is designed. According to the distribution of lateral stress in working face, eight pillars of different sizes are designed. By simulating and comparing the stress distribution of surrounding rock and the development range and shape of plastic zone in different positions, the pillar size of gob-side entry driving is optimized to be 4.5m. According to the results of optimization of roadway section, the section of straight wall semi-circular arch roadway is adopted. According to the analysis, the roadway is supported by bolt + steel mesh + anchor cable. By observing the stability of roadway, it provides experience for the stability study of roadway the gob-side entry driving with small pillar in thick seam.
A Study of Reproducibility of Papanicolaou Society of Cytopathology System of Reporting Respiratory Cytology-A Single Institutional Experience on Image Guided Aspiration Cytology
Authors: Patel Surbhi , Adiga Deepa , Rai Sharada 
Published: December 2021

Abstract
Background: Cytologic examination of specimens obtained from the respiratory tract is a primary and frequently the initial diagnostic technique performed in patients with pulmonary abnormalities. Fine needle aspiration (FNA) is extensively used for diagnosis of pulmonary lesions. The Papanicolaou Society of Cytopathology (PSC) issued a new classification for respiratory cytology and criteria, risk of malignancy, post-cytologic diagnosis management and follow-up. Methods: Respiratory FNA specimens obtained between January 2015 to March 2021 were reviewed and reclassified according to PSC guidelines. Cytologic category as per PSC system was assigned after reviewing by two pathologists. Risk of malignancy for each category was calculated for cases where biopsy was available for correlation. Results: Three eighty-four samples were classified as non-diagnostic (30.5%), negative for malignancy (13.3%), atypical (0.5%), neoplastic (benign/ low malignant potential) (0%), suspicious for malignancy (5.7%%) and malignancy (50%). Risk of malignancy for malignant category (Category V and Category VI) was 64.1% and for non-malignant (Category I to Category IV) cases was 35.9%. Overall, there was lesser incidence of atypical category and more specific diagnoses were possible on cytology in non-malignant category. Sensitivity and specificity for malignant cases was 99.33% and 100% respectively. Conclusion: Our study substantiates that use of PSC guidelines improves the overall reporting of respiratory cytology due to the use of standardized terminology.
Azospirillum Actinidiae sp. nov., a Nitrogen-Fixing Bacterium Isolated From The Roots of Kiwifruit Plants
Authors: Yang Ying , Zhu Yue , Li Wei , Ren Yajing , Huang Shengxiong , Liu Yongsheng 
Published: December 2021

Abstract
A novel diazotrophic bacterium, designated CCTCC AB 2021101T, was isolated from fresh roots of kiwifruit. Cells of strain CCTCC AB 2021101T were Gram-negative, aerobic and rod-shaped, with motility provided by peritrichous flagella. The 16S rRNA analysis showed that strain CCTCC AB 2021101T belongs to the genus Azospirillum and is closely related to Azospirillum melinis (98.32%), Azospirillum oryzae (97.73%), Azospirillum lipoferum (96.98%), Azospirillum humicireducens (96.49%) and Azospirillum largimobile (96.01%) and lower sequence similarity (<96.0 %) to all other species of the genus Azospirillum. Strain CCTCC AB 2021101T was able to grow well at 35–40℃ and pH 6.0–7.0, and tolerated up to 3.0 % (w/v) NaCl. The major saturated fatty acids are C14:0, C16:0 and C18:0. C18:1 ω7c and C16:0 3-OH were the major unsaturated and hydroxylated fatty acid. The G+C content was 67.8 mol%. Strain CCTCC AB 2021101T gave positive amplification for dinitrogen reductase (nifH gene). Highest nifH gene sequence similarities were obtained with Azospirillum brasilense AWB14T(95.9%), Azospirillum zeae Gr24T(95.56%), Azospirillum picis DSM 19922T(96.79%), Azospirillum lipoferum B22T(94.88%) and Azospirillum oryzae COC8T(94.88%). The activity of the nitrogenase of the strain was further confirmed by acetylene-reduction assay, which was recorded as 81 nmol ethylene h-1. Based on these data, strain CCTCC AB 2021101T is considered to represent a novel endophytic diazotrophs species in the genus Azospirillum, for which the name Azospirillum actinidiae sp. nov. is proposed. The type strain is CCTCC AB 2021101T.
Dynamic Zero-Covid strategy curtails mutagenesis and emergence of new variants of the SARS-CoV-2
Authors: Cui Lunbiao , Zhu Liguo , Zhang Jun , Fan Huafeng , Yi Yongxiang , Zhao Jun , Ge Yiyue , Wang Shenjiao , Han Xu , Gao Xingsu , Qi Xian , Fu Jianguang , Li Zhifeng , Fan Huan , Yu Huiyan , Deng Fei , Zhao Kangchen , Zhu Xiaojuan , Hu Jianli , Li Jianjun , Ai Jing , Kang Guodong , Shen Ya , Jin Guangjie , Wang Furu , Zhang Zhong , He Min , Ding Songning , Wang Yin , Dong Yuying , Huang Yao , Yi Changhua , Wang Longyu , Jiao Yudong , Wang Jinfu , Li Jian , Liu Hongde , Li Jingxin , Bao Changjun , Wu Ming , Zhu Fengcai 
Published: December 2021

Abstract
Within the local outbreak period of SARS-CoV-2 Delta variant in Nanjing and Yangzhou, China, we analyzed the mutation process of the Delta variants in 520 cases, as well as the production, spread and elimination of new mutant strains under the non-pharmaceutical interventions (NPI) strategy. The investigation on distribution of COVID-19 cases and phylogenetic analysis of SARS-CoV-2 genome sequences attributed to tracking the transmission chains, transmission chains were terminated by the isolation of the COVID-19 patients and quarantine of close-contracts, suggesting the importance of NPI in prompting some mutations to disappear and stopping the transmission of new variants. Dynamic zero-Covid strategy has been implemented successfully to against the second-largest local epidemic caused by an imported COVID-19 case in China.
Circular RNA CircCSPP1 Promotes the Occurrence and Development of Colon Cancer by Sponging miR-431 and Regulating the Expressions of ROCK1 and ZEB1
Authors: Wang Jin , Zhou Lei , Chen Bingxin , Yu Zhuwen , Zhang Jianglei , Zhang Zhe , Hu Chenrui , Bai Yanjin , Ruan Xiaokang , Wang Shengjia , Ouyang Jun , Wu Airong , Zhao Xin 
Published: December 2021

Abstract
Colon cancer is a common malignant tumor of the digestive tract, and its incidence is ranked third among gastrointestinal tumors. The present study investigated the role of a novel circular RNA (circCSPP1) in colon cancer and explored the possible underlying molecular mechanisms. Bioinformatics analysis and reverse transcription-quantitative PCR were used to detect the expression levels of circCSPP1 in colon cancer specimens and cell lines. The effects of circCSPP1 on the behavior of colon cancer cells were investigated using CCK-8, Transwell and clonogenic assays. Bioinformatics analysis along with luciferase, fluorescence in situ hybridization and RNA pull-down assays were used to reveal the interaction between circCSPP1, microRNA (miR)-431, Rho associated coiled-coil containing protein kinase 1 (ROCK1) and zinc finger E-box binding homeobox 1 (ZEB1). It was found that circCSPP1 expression was significantly upregulated in colon cancer tissues and cell lines. The overexpression of circCSPP1 significantly promoted the proliferation, migration and invasion of colon cancer cells, whereas the silencing of circCSPP1 exerted opposite effects. Mechanistically, circCSPP1 was found to bind with miR-431. In addition, ROCK1 and ZEB1 were identified as the target genes of miR-431. Rescue experiments further confirmed the interaction between circCSPP1, miR-431, ROCK1 and ZEB1. Moreover, circCSPP1 promoted the expression levels of ROCK1, cyclin D1, cyclin-dependent kinase 4, ZEB1 and Snail, and lowered the E-cadherin expression level. Notably, circCSPP1 from SW620 cells was transferred to macrophages via exosomes and enhanced the colon cancer microenvironment. Taken together, the findings of the present study indicated that circCSPP1 may functions as a competing endogenous RNA in the progression of colon cancer by regulating the miR-431/ROCK1 and miR-431/ZEB1 signaling axes.
What is Polypharmacy In People Living With HIV/AIDS? A Systematic Review
Authors: Danjuma Mohammed Ibn-Mas’ud , Khan Safah , Wahbeh Farah , Naseralallah Lina Mohammad , Elzouki Abdelnaser 
Published: December 2021

Abstract
Polypharmacy in people living with HIV/AIDS (PLWHA) is a rising morbidity that exacts hefty economic burden on health budgets in addition to other adverse clinical outcomes. Despite recent attempts, uncertainty remains around its exact definition in PLWHA. In this synthesis, we explored relevant databases (PUBMED, EMBASE, CROI) for studies evaluating polypharmacy in PLWHA from January 2000 to August 2021. Two independent reviewers extracted and reviewed relevant variables for analyses. The review included a total of 31 studies involving n = 53347 with a mean age of 49.5 (SD±17.0) years. There was a total of 36 definitions, with 93.5% defining polypharmacy as the concomitant use of 5 or more medications. We found significant variation in the numerical definition of polypharmacy, with studies reporting it as “minor” (N = 3); “major” (N = 29); “severe” (N = 2); “excessive” (N = 1); and “higher” (N = 1). Most studies did not incorporate a duration (84%) in their definition and excluded ART medications (67.7%). A plurality of studies in PLWHA have established that polypharmacy in this cohort of patients is the intake of ≥5 non-ART medications. To standardize the approach to addressing this rising morbidity, we recommend incorporation of this definition into national and international PLWHA treatment guidelines.