bluuebunny/crossref_metadata_2025_split huggingface dataset:
Study on the Present Status of Endangered Fishes and Productivity of Teesta River Closest to Barrage Region
Authors: Amin AKM Rohul , Shah Md Rakibuzzaman , Alam Md Mahmood , Hoshan Imran , Zafar Md Abu 
Published: March 2021

This study was conducted to monitor the present condition of endangered fishes and productivity of Teesta river closest to Teesta barrage situated in the Lalmonirhat district of Bangladesh. Water and sediment samples were collected twice in a month during the study period from six different (3 upstream and 3 downstream) sites with three replications for each. Required information about threatened fishes was collected from the sampling region associated fishermen and fish markets. The study disclosed over 50 threatened fish species in Teesta river including several threatened fishes namely Bagarius bagarius, Sisor rabdophorus etc. The commonly available endangered fishes were Macrognathus aculiatus, Mastacembelus armatus, Barilius tileo, Raiamas bola, Botia dario, B. lohachata etc. which are rarely available in nationwide. Planktonic flora and fauna determination revealed that comparatively higher density of plankton (n >11500 per liter) as well as more number of planktonic flora (>21 nos.) and fauna (>9 nos.) were monitored in the early monsoon and monsoon season (April-September) and comparatively lower planktonic density (n<10000 per liter) and less number were found in premonsoon season (January-February). The investigation of benthic fauna showed that the riverine ecosystem near to barrage contained 16 species of macro-benthos from different groups. Lastly, it can be noticed that it is very essential to take all effective necessary actions to provide good productivity and conserve the ichthyo diversity of Teesta river that will help to conserve the commonly available endangered and critically endangered fishes of Teesta river.
Res. Agric., Livest. Fish.7(3): 577-589, December 2020
Propuesta de reintegración de productos autóctonos en la canasta básica: caso tortillas de zapallo
Authors: Ríos-Mariño Marcelo , Herrera-Caicedo Ana , Cajas-Cajas Viviana , Jijón-Argüello Washington 
Published: March 2021

Las personas cada día están más preocupadas por su nutrición sin embargo, a la vez disponen de poco tiempo para la preparación de alimentos saludables para su consumo diario, es por eso que se ofrece un producto nuevo en el mercado siendo éste las Tortitas Kürbis que son tortillas de zapallo, el cual es un producto autóctono con alto nivel nutritivo y que ofrece un sin número de beneficios para la salud, entre ellos: ayuda a la digestión, aumenta el nivel de energía, previene la anemia, ayuda a la hipertensión y mejora el cuidado de la piel. La investigación de carácter descriptivo se enfoca en Parroquias Urbanas de la Administración Zonal Eugenio Espejo del D.M. de Quito, para la cual se utilizó la técnica de la encuesta. El estudio de mercado demuestra que existe una demanda insatisfecha frente a una oferta indirecta; además, se establece que el precio del paquete de 10 unidades más accesible para el consumidor potencial es de $ 1,50. Por otro lado, se hace un análisis de las fortalezas, debilidades, oportunidades y amenazas del cual se desprende la matriz de estrategias. Se concluye demostrando la factibilidad comercial del producto y se plantea a futuro continuar con el estudio económico.
Longitudinal Assessment of Immune Responses to Repeated Annual Influenza Vaccination in a Human Cohort of Adults and Teenagers
Authors: Sung Meng-Hsuan , Shen Ye , Handel Andreas , Bahl Justin , Ross Ted M. 
Published: March 2021

Background: The overall performance of a multiple component vaccine assessed by the vaccine-elicited immune responses across various strains in a repeated vaccination setting has not been well-studied, and the comparison between adults and teenagers is yet to be made.Methods: A human cohort study was conducted at the University of Georgia, with 140 subjects (86 adults and 54 teenagers) repeatedly vaccinated in the 2017/2018 and 2018/2019 influenza seasons. Host information was prospectively collected, and serum samples were collected before and after vaccination in each season. The association between host factors and repeated measures of hemagglutination inhibition (HAI) composite scores was assessed by generalized linear models with generalized estimating equations.Results: The mean HAI composite scores for the entire sample (t = 4.26, df = 139, p &lt; 0.001) and the teenager group (t = 6.44, df = 53, p &lt; 0.001) declined in the second season, while the changes in the adults were not statistically significant (t = −1.14, df = 85, p = 0.26). A mixture pattern of changes in both directions was observed in the adults when stratified by prior vaccination. In addition, the regression analysis suggested an interactive effect of age and BMI on the HAI composite scores in the overall population (beta = 0.005; 95% CI, 0.0008–0.01) and the adults (beta = 0.005; 95% CI, 0.0005–0.01).Conclusions: Our study found distinct vaccine-elicited immune responses between adults and teenagers when both were repeatedly vaccinated in consecutive years. An interactive effect of age and BMI on the HAI composite scores were identified in the overall population and the adults.
Ipsilateral Motor Evoked Potentials as a Measure of the Reticulospinal Tract in Age-Related Strength Changes
Authors: Maitland Stuart , Baker Stuart N. 
Published: March 2021

Background: The reticulospinal tract (RST) is essential for balance, posture, and strength, all functions which falter with age. We hypothesized that age-related strength reductions might relate to differential changes in corticospinal and reticulospinal connectivity.Methods: We divided 83 participants (age 20–84) into age groups &lt;50 (n = 29) and ≥50 (n = 54) years; five of which had probable sarcopenia. Transcranial Magnetic Stimulation (TMS) was applied to the left cortex, inducing motor evoked potentials (MEPs) in the biceps muscles bilaterally. Contralateral (right, cMEPs) and ipsilateral (left, iMEPs) MEPs are carried by mainly corticospinal and reticulospinal pathways respectively; the iMEP/cMEP amplitude ratio (ICAR) therefore measured the relative importance of the two descending tracts. Grip strength was measured with a dynamometer and normalized for age and sex.Results: We found valid iMEPs in 74 individuals (n = 44 aged ≥50, n = 29 &lt; 50). Younger adults had a significant negative correlation between normalized grip strength and ICAR (r = −0.37, p = 0.045); surprisingly, in older adults, the correlation was also significant, but positive (r = 0.43, p = 0.0037).Discussion: Older individuals who maintain or strengthen their RST are stronger than their peers. We speculate that reduced RST connectivity could predict those at risk of age-related muscle weakness; interventions that reinforce the RST could be a candidate for treatment or prevention of sarcopenia.
Preanalytical Stability of CSF Total and Oligomeric Alpha-Synuclein
Authors: Abdi Ilham Y. , Majbour Nour K. , Willemse Eline A. J. , van de Berg Wilma D. J. , Mollenhauer Brit , Teunissen Charlotte E. , El-Agnaf Omar M. 
Published: March 2021

Background: The role of cerebrospinal fluid (CSF) alpha-synuclein as a potential biomarker has been challenged mainly due to variable preanalytical measures between laboratories. To evaluate the impact of the preanalytical factors contributing to such variability, the different subforms of alpha-synuclein need to be studied individually.Method: We investigated the effect of exposing CSF samples to several preanalytical sources of variability: (1) different polypropylene (PP) storage tubes; (2) use of non-ionic detergents; (3) multiple tube transfers; (4) multiple freeze-thaw cycles; and (5) delayed storage. CSF oligomeric- and total-alpha-synuclein levels were estimated using our in-house sandwich-based enzyme-linked immunosorbent assays.Results: Siliconized tubes provided the optimal preservation of CSF alpha-synuclein proteins among other tested polypropylene tubes. The use of tween-20 detergent significantly improved the recovery of oligomeric-alpha-synuclein, while multiple freeze-thaw cycles significantly lowered oligomeric-alpha-synuclein in CSF. Interestingly, oligomeric-alpha-synuclein levels remained relatively stable over multiple tube transfers and upon delayed storage.Conclusion: Our study showed for the first-time distinct impact of preanalytical factors on the different forms of CSF alpha-synuclein. These findings highlight the need for special considerations for the different forms of alpha-synuclein during CSF samples’ collection and processing.
Two Classes of T1 Hypointense Lesions in Multiple Sclerosis With Different Clinical Relevance
Authors: Kocsis Krisztián , Szabó Nikoletta , Tóth Eszter , Király András , Faragó Péter , Kincses Bálint , Veréb Dániel , Bozsik Bence , Boross Katalin , Katona Melinda , Bodnár Péter , László Nyúl Gábor , Vécsei László , Klivényi Péter , Bencsik Krisztina , Kincses Zsigmond Tamás 
Published: March 2021

Background: Hypointense lesions on T1-weighted images have important clinical relevance in multiple sclerosis patients. Traditionally, spin-echo (SE) sequences are used to assess these lesions (termed black holes), but Fast Spoiled Gradient-Echo (FSPGR) sequences provide an excellent alternative.Objective: To determine whether the contrast difference between T1 hypointense lesions and the surrounding normal white matter is similar on the two sequences, whether different lesion types could be identified, and whether the clinical relevance of these lesions types are different.Methods: Seventy-nine multiple sclerosis patients' lesions were manually segmented, then registered to T1 sequences. Median intensity values of lesions were identified on all sequences, then K-means clustering was applied to assess whether distinct clusters of lesions can be defined based on intensity values on SE, FSPGR, and FLAIR sequences. The standardized intensity of the lesions in each cluster was compared to the intensity of the normal appearing white matter in order to see if lesions stand out from the white matter on a given sequence.Results: 100% of lesions on FSPGR images and 69% on SE sequence in cluster #1 exceeded a standardized lesion distance of Z = 2.3 (p &lt; 0.05). In cluster #2, 78.7% of lesions on FSPGR and only 17.7% of lesions on SE sequence were above this cutoff value, meaning that these lesions were not easily seen on SE images. Lesion count in the second cluster (lesions less identifiable on SE) significantly correlated with the Expanded Disability Status Scale (EDSS) (R: 0.30, p ≤ 0.006) and with disease duration (R: 0.33, p ≤ 0.002).Conclusion: We showed that black holes can be separated into two distinct clusters based on their intensity values on various sequences, only one of which is related to clinical parameters. This emphasizes the joint role of FSPGR and SE sequences in the monitoring of MS patients and provides insight into the role of black holes in MS.
Descriptive Analysis of Real-World Data on Fingolimod Long-Term Treatment of Young Adult RRMS Patients
Authors: Ziemssen Tjalf , Albrecht Holger , Haas Judith , Klotz Luisa , Lang Michael , Lassek Christoph , Schmidt Stephan , Ettle Benjamin , Schulze-Topphoff Ulf 
Published: March 2021

Background: Fingolimod (Gilenya®) is approved for adult and pediatric patients with highly active relapsing–remitting multiple sclerosis (RRMS).Objectives: The objective was to describe the effectiveness of fingolimod in young adults compared to older patients in clinical practice.Methods: PANGAEA is the largest prospective, multi-center, non-interventional, long-term study evaluating fingolimod in RRMS. We descriptively analyzed demographics, MS characteristics, and severity in two subgroups of young adults (≤20 and &gt;20 to ≤30 years) and older patients (&gt;30 years).Results: Young adults had lower Expanded Disability Status Scale (EDSS) scores compared to older patients (1.8 and 2.3 vs. 3.2) at baseline. The mean EDSS scores remained stable over 5 years in all subgroups. Young adults had higher annual relapse rates (2.0 and 1.7 vs. 1.4) at study entry, which were reduced by approximately 80% in all subgroups over 5 years. The proportion of patients with no clinical disease activity in year 4 was 52.6 and 73.4 vs. 66.9% in patients ≤20, &gt;20 to ≤30 years and &gt;30 years, respectively. The symbol digit modalities test score increased by 15.25 ± 8.3 and 8.3 ± 11.3 (mean ± SD) from baseline in patients &gt;20 to ≤30 and &gt;30 years.Conclusions: Real-world evidence suggests a long-term treatment benefit of fingolimod in young RRMS patients.
Diffusion Tensor Imaging of the Sciatic Nerve as a Surrogate Marker for Nerve Functionality of the Upper and Lower Limb in Patients With Diabetes and Prediabetes
Authors: Jende Johann M. E. , Kender Zoltan , Mooshage Christoph , Groener Jan B. , Alvarez-Ramos Lucia , Kollmer Jennifer , Juerchott Alexander , Hahn Artur , Heiland Sabine , Nawroth Peter , Bendszus Martin , Kopf Stefan , Kurz Felix T. 
Published: March 2021

BackgroundNerve damage in diabetic neuropathy (DN) is assumed to begin in the distal legs with a subsequent progression to hands and arms at later stages. In contrast, recent studies have found that lower limb nerve lesions in DN predominate at the proximal sciatic nerve and that, in the upper limb, nerve functions can be impaired at early stages of DN.Materials and MethodsIn this prospective, single-center cross-sectional study, participants underwent diffusion-weighted 3 Tesla magnetic resonance neurography in order to calculate the sciatic nerve’s fractional anisotropy (FA), a surrogate parameter for structural nerve integrity. Results were correlated with clinical and electrophysiological assessments of the lower limb and an examination of hand function derived from the Purdue Pegboard Test.ResultsOverall, 71 patients with diabetes, 11 patients with prediabetes and 25 age-matched control subjects took part in this study. In patients with diabetes, the sciatic nerve’s FA showed positive correlations with tibial and peroneal nerve conduction velocities (r = 0.62; p &lt; 0.001 and r = 0.56; p &lt; 0.001, respectively), and tibial and peroneal nerve compound motor action potentials (r = 0.62; p &lt; 0.001 and r = 0.63; p &lt; 0.001, respectively). Moreover, the sciatic nerve’s FA was correlated with the Pegboard Test results in patients with diabetes (r = 0.52; p &lt; 0.001), prediabetes (r = 0.76; p &lt; 0.001) and in controls (r = 0.79; p = 0.007).ConclusionThis study is the first to show that the sciatic nerve’s FA is a surrogate marker for functional and electrophysiological parameters of both upper and lower limbs in patients with diabetes and prediabetes, suggesting that nerve damage in these patients is not restricted to the level of the symptomatic limbs but rather affects the entire peripheral nervous system.
Anti-Tumoral and Anti-Angiogenic Effects of Low-Diluted Phenacetinum on Melanoma
Authors: Fuselier Camille , Quemener Sandrine , Dufay Eleonore , Bour Camille , Boulagnon-Rombi Camille , Bouland Nicole , Djermoune El-Hadi , Devy Jérôme , Martiny Laurent , Schneider Christophe 
Published: March 2021

Melanoma is the most aggressive form of skin cancer and the most rapidly expanding cancer in terms of worldwide incidence. If primary cutaneous melanoma is mostly treated with a curative wide local excision, malignant melanoma has a poor prognosis and needs other therapeutic approaches. Angiogenesis is a normal physiological process essential in growth and development, but it also plays a crucial role in crossing from benign to advanced state in cancer. In melanoma progression, angiogenesis is widely involved during the vertical growth phase. Currently, no anti-angiogenic agents are efficient on their own, and combination of treatments will probably be the key to success. In the past, phenacetin was used as an analgesic to relieve pain, causing side effects at large dose and tumor-inducing in humans and animals. By contrast, Phenacetinum low-dilution is often used in skin febrile exanthema, patches profusely scattered on limbs, headache, or flushed face without side effects. Herein are described the in vitro, in vivo, and ex vivo anti-angiogenic and anti-tumoral potentials of Phenacetinum low-dilution in a B16F1 tumor model and endothelial cells. We demonstrate that low-diluted Phenacetinum inhibits in vivo tumor growth and tumor vascularization and thus increases the survival time of B16F1 melanoma induced-C57BL/6 mice. Moreover, Phenacetinum modulates the lung metastasis in a B16F10 induced model. Ex vivo and in vitro, we evidence that low-diluted Phenacetinum inhibits the migration and the recruitment of endothelial cells and leads to an imbalance in the pro-tumoral macrophages and to a structural malformation of the vascular network. All together these results demonstrate highly hopeful anti-tumoral, anti-metastatic, and anti-angiogenic effects of Phenacetinum low-dilution on melanoma. Continued studies are needed to preclinically validate Phenacetinum low-dilution as a complementary or therapeutic strategy for melanoma treatment.
Case Report: Favorable Response to the Tyrosine Kinase Inhibitor Apatinib in Recurrent Merkel Cell Carcinoma
Authors: Feng Yiyi , Song Xin , Jia Renbing 
Published: March 2021

BackgroundAs angiogenesis is an essential step in tumor growth and metastasis, the tyrosine kinase inhibitor (TKI) apatinib has become a revolutionary anticancer therapy across various malignancies. However, its efficiency and safety in Merkel cell carcinoma (MCC) are uncertain.Case presentationThe current study described the case of a 91-year-old man who presented with a 3.2 × 3.0 × 2.2 cm rapidly growing, solitary tumor of the right lower eyelid. It was diagnosed as MCC pathologically. Twenty-seven days after the surgery, the patient returned to the hospital with recurrent MCC. Apatinib was then administered to this patient. The patient had a complete response (CR) to apatinib after 4.4 months of targeted therapy. Twenty-seven months of progression-free survival (PFS) was achieved with controllable treatment-related adverse events (AEs).ConclusionTreatment with apatinib demonstrated clinical benefit in our patient with recurrent MCC, highlighting its potential utility in other MCC patients. Further clinical trials are needed to determine the efficacy and safety of apatinib in MCC patients.
Tumor Immune Microenvironment Characteristics and Their Prognostic Value in Non-Small-Cell Lung Cancer
Authors: Su Dan , Wu Gao , Xiong Ran , Sun Xiangxiang , Xu Meiqing , Mei Yide , Wu Xianning 
Published: March 2021

IntroductionCancer progression is determined not only by the malignant behavior of tumors but also by the immune microenvironment. The tumor immune microenvironment also plays a pivotal role in determining the clinical response of non-small-cell lung cancer (NSCLC) to immunotherapies. To understand the possible mechanisms and explore new targets in lung cancer immunotherapy, we characterized the immune profiles in NSCLC patients.MethodsSeventy-one NSCLC patients who underwent radical resection were selected. The immune cell composition in paired tumor and adjacent normal lung tissues was tested by flow cytometry. The associations of tumor immune microenvironment characteristics with clinicopathological factors and overall survival were analyzed. Kaplan–Meier curves and Cox proportional hazards models were used to determine differences in survival.ResultsCompared with adjacent normal lung tissues, an increased proportion of CD45+ hematopoietic-derived cells, CD4+ T cell subtypes, Tregs and B cells was observed in tumor samples with a reduced frequency of myeloid cell populations. There was no significant increase in total CD8+ T cells, but both PD1+ and CD38+ CD8+ T cells were significantly enriched in tumor samples and statistically significantly associated with tumor size. In addition, positive CD38 expression was highly correlated with PD1 positivity. A high proportion of CD8+ T cells and a low percentage of PD1+ CD8+ T cells were statistically significantly associated with better survival in stage II and III patients, whereas a low frequency of CD38+ CD8+ T cells was statistically significantly associated with better survival in all patients and identified as an independent prognostic factor (p=0.049).ConclusionWe profiled the immune cells in the tumor tissues of NSCLC patients using flow cytometry. The results revealed significant enrichment of infiltrating immune cells. A strong correlation was identified between CD38 and PD-1 expression on CD8+ T cells in tumors. CD8+ T cells and their subtypes play a critical role in the prediction of prognosis.
A Lymph Node Count-Based AJCC Staging System Facilitates a More Accurate Prediction of the Prognosis of Patients With Endometrial Cancer
Authors: Huo Xinlong , Wang Shufang 
Published: March 2021

PurposeBoth the International Federation of Gynecology and Obstetrics (FIGO) and the American Joint Committee on Cancer (AJCC) staging system for endometrial cancer (EC) defined the N category by the location of metastatic lymph nodes (LNs) rather than the metastatic LN count. We aimed to compare the accuracy of the AJCC staging system and the LN count-based staging system.Patients and MethodsEC patients were selected from the Surveillance, Epidemiology and End Results (SEER) database between 2004 and 2016. Patients’ characteristics were collected, including age, race, marital status, histological type, grade, therapeutic measures, the number of metastatic LNs, the number of dissected LNs, vital status, and survival in months. Overall survival (OS) was analyzed by the Kaplan–Meier (KM) method and the concordance index (C-index) was used to compare the prognostic value of the AJCC staging system and the LN count-based staging system.ResultsWe identified 4,276 EC cases from the SEER database, including 2,693 patients with stage IIIC1 and 1,583 patients with stage IIIC2. Multivariate analyses showed that independent prognostic factors for patients with stage IIIC1 included age, race, marital status, grade, histology, chemotherapy, and radiotherapy. Independent prognostic factors for patients with stage IIIC2 included age, marital status, grade, histology, chemotherapy, and radiotherapy. The C-index of the AJCC staging system and the LN count-based staging system were 0.483 and 0.617, respectively. At least six LNs should be dissected to ensure the accuracy of the LN count-based staging system.ConclusionA modified AJCC staging system based on the count of metastatic LNs might be superior to the current AJCC staging system, which still had room for improvement and further refinements were required. For accurate staging, we recommended that at least six LNs should be examined in the modified AJCC staging system.