bluuebunny/crossref_metadata_2025_split huggingface dataset:
Analysis of the Yarrowia
lipolytica proteome reveals subtle variations in expression levels between lipogenic and non-lipogenic conditions
Authors: Sestric Ryan , Spicer Vic , V. Krokhin Oleg , Sparling Richard , B. Levin David 
Published: February 2021

ABSTRACT
Oleaginous yeasts have the ability to store greater than 20% of their mass as neutral lipids, in the form of triacylglycerides. The ATP citrate lyase is thought to play a key role in triacylglyceride synthesis, but the relationship between expression levels of this and other related enzymes is not well understood in the role of total lipid accumulation conferring the oleaginous phenotype. We conducted comparative proteomic analyses with the oleaginous yeast, Yarrowia lipolytica, grown in either nitrogen-sufficient rich media or nitrogen-limited minimal media. Total proteins extracted from cells collected during logarithmic and late stationary growth phases were analyzed by 1D liquid chromatography, followed by mass spectroscopy. The ATP citrate lyase enzyme was expressed at similar concentrations in both conditions, in both logarithmic and stationary phase, but many upstream and downstream enzymes showed drastically different expression levels. In non-lipogenic conditions, several pyruvate enzymes were expressed at higher concentration. These enzymes, especially the pyruvate decarboxylase and pyruvate dehydrogenase, may be regulating carbon flux away from central metabolism and reducing the amount of citrate being produced in the mitochondria. While crucial for the oleaginous phenotype, the constitutively expressed ATP citrate lyase appears to cleave citrate in response to carbon flux upstream from other enzymes creating the oleaginous phenotype.
Restoration thinning reduces bush encroachment on freehold farmlands in north-central Namibia
Authors: Nghikembua Matti T , Marker Laurie L , Brewer Bruce , Leinonen Arvo , Mehtätalo Lauri , Appiah Mark , Pappinen Ari 
Published: February 2021

Abstract
Bush encroachment affects ~45 million ha of Namibia and, without appropriate restoration measures, it negatively affects rangeland productivity and biodiversity. Thinning is a common method to counteract bush encroachment. The thinning strategy applied in north-central Namibia was assessed to examine how effective it has been in reducing bush encroachment. Trees/shrubs were selectively thinned manually, targeting all height classes, except individuals with stem diameters ≥18 cm. We investigated the effects on the vegetation and soil properties using surveys on three freehold farms (in 2016 and 2017) in bush-encroached and previously thinned habitats. Our results revealed significant differences in the mean total nitrogen (TN) content between the treatments; thinned areas had higher TN content which would be beneficial for fast-growing grasses. In the thinned plots, the occurrence probability of red umbrella thorn (Vachellia reficiens Warwa) was significantly reduced, indicating that it was the most harvested species; and umbrella thorn (Vachellia tortilis (Burch.) Brenan spp. heteracantha) was increased, indicating that it favoured reduced densities of dominant species. Natural regeneration was rapid; the tree/shrub abundance in the 0–1-m height class in the thinned area surpassed those in the non-thinned by 34 per cent, ~7.2 years since thinning. Thinning significantly reduced tree/shrub abundances of the 1–3- and &gt;3-m height classes, which was still evident 7.2 years since thinning. Based upon the generalized linear mixed-effects model, tree/shrub counts between treatments may equalize in ~14 and 15 years for the 1–3- and &gt;3-m height classes, respectively. Thinning was effective in reducing tree/shrub abundances and can be used to restore wildlife habitat on the Namibian farmland: however, post-thinning management is required to maintain an open savannah vegetation structure as the 0–1-m height class cohort will eventually grow into mature trees/shrubs.
Typologies of Dementia Caregiver Support Networks: A Pilot Study
Authors: Friedman Esther M , Kennedy David P 
Published: February 2021

AbstractBackground and ObjectivesThere are nearly 18 million family caregivers in the United States assisting an older adult in need of help. Identifying the caregivers in greatest need of support requires an understanding of the current social support networks available to family caregivers and whether specific groups of caregivers are at risk of having an insufficient support network.Research Design and MethodsWe collected personal network data from a nationally representative sample of 66 family caregivers to persons with dementia (PWDs) in the United States aged 18 and older, including information on network members’ support to the caregiver and help to the care recipient.ResultsWe found four common caregiving network types: large networks with many helpers; large networks primarily supporting caregivers; small, dense networks supporting both caregivers and care recipient; and small networks providing little help to either caregiver or care recipient. Gender, income, and geographic proximity of caregiver to the care recipient were significantly associated with caregiver network type.Discussion and ImplicationsThis study suggests that there are different types of care and support networks available to caregivers to PWDs, and that the size and structure of networks vary considerably among demographic groups. As the population ages, a better understanding of the supports available to caregivers will be crucial for ensuring that caregivers are adequately supported, and caregiving needs of families are met.
The dual aversion of Chile’s constitution-making process
Authors: Verdugo Sergio , Prieto Marcela 
Published: February 2021

Abstract
Chile initiated a constitution-making process in late 2019, after the major political parties signed an agreement to respond to the massive demonstrations that took over the streets in October of 2019. Dominant trends in Chile and Latin America’s constitutional thought typically examine this type of process through the lenses of the constituent power or transformative constitutionalism. The authors of this essay offer a different view. They argue that Chile’s constitution-making process, as designed by the multiparty agreement, manifests a double aversion: to avoid the Bolivarian way of constitution-making—including its associated constituent power narrative—and to put an end to the institutional and symbolic legacy of the Pinochet regime. In attempting to stay clear of these two negative models, the authors argue that the rules of the constitution-making process have adopted the main features of the post-sovereign model of constitution-making.
Hyponatremia Following Antipsychotic Treatment: In Silico Pharmacodynamics Analysis of Spontaneous Reports From the US Food and Drug Administration Adverse Event Reporting System Database and an Updated Systematic Review
Authors: Mazhar Faizan , Battini Vera , Pozzi Marco , Invernizzi Elena , Mosini Giulia , Gringeri Michele , Capuano Annalisa , Scavone Cristina , Radice Sonia , Clementi Emilio , Carnovale Carla 
Published: February 2021

Abstract

Background
Hyponatremia associated with antipsychotic drugs is a rare but potentially life-threatening adverse drug reaction; the underlying pharmacological mechanism has not yet been explained.

Methods
We investigated the relationship between pharmacological targets of antipsychotic drugs and the occurrence of hyponatremia by conducting a nested case-control study using the Food and Drug Administration Adverse Event Reporting System database. Multiple logistic regression was used to determine the associations between antipsychotics receptor occupancy and hyponatremia. We also performed a systematic review of clinical studies on this association.

Results
Of 139 816 reports involving at least 1 antipsychotic, 1.1% reported hyponatremia. Olanzapine was the most frequently suspected drug (27%). A signiﬁcant positive association was found between dopamine D3, D4, and hyponatremia, while adrenergic α 1, serotonin 5-HT1A, and 5-HT2A receptor occupancies were negatively associated. A multivariable stepwise regression model showed that dopamine D3 (adj. odds ratio = 1.21; 95% CI = 1.09–1.34; P &lt; .05) predicted the risk for hyponatremia (P &lt; .05), while serotonin 5-HT2A occupancy (Adj. odds ratio = 0.78; 95% CI = 0.68–0.90; P &lt; .01) exhibited a protective effect against hyponatremia. Among the 11 studies included in the systematic review, incidence rates of hyponatremia diverged between 0.003% and 86%, whereas the odds of developing hyponatremia from effect studies ranged between 0.83 and 3.47.

Conclusions
Antipsychotic drugs having a combined modest occupancy for D3 and 5-HT2A receptors and higher levels of D3 receptor occupancy correspond to different degrees of risk for hyponatremia. Based on the few, relatively large-scale available studies, atypical antipsychotics have a more attenuated risk proﬁle for hyponatremia.
IS26cannot move alone
Authors: Harmer Christopher J , Hall Ruth M 
Published: February 2021

AbstractBackgroundIS26 plays a major role in the dissemination of antibiotic resistance determinants in Gram-negative bacteria.ObjectivesTo determine whether insertion sequence IS26 is able to move alone (simple transposition) or if it exclusively forms cointegrates.MethodsA two-step PCR using outward-facing primers was used to search for circular IS26 molecules. Gibson assembly was used to clone a synthetic IS26 containing a catA1 chloramphenicol resistance gene downstream of the tnp26 transposase gene into pUC19. IS activity in a recA−Escherichia coli containing the non-conjugative pUC19-derived IS26::catA1 construct and the conjugative plasmid R388 was detected using a standard mating-out assay. Transconjugants were screened for resistance.ResultsCircular IS26 molecules that would form with a copy-out route were not detected by PCR. The synthetic IS26::catA1 construct formed CmRTpR transconjugants (where CmR and TpR stand for chloramphenicol resistant and trimethoprim resistant, respectively), representing an R388 derivative carrying the catA1 gene at a frequency of 5.6 × 10−7 CmRTpR transconjugants per TpR transconjugant, which is comparable to the copy-in activity of the unaltered IS26. To test for simple transposition of IS26::catA1 (without the plasmid backbone), 1200 CmRTpR colonies were screened and all were resistant to ampicillin, indicating that the pUC19 backbone was present. Hence, IS26::catA1 had only formed cointegrates.ConclusionsIS26 is unable to move alone and cointegrates are the exclusive end products of the reactions mediated by the IS26 transposase Tnp26. Consequently, when describing the formation of complex resistance regions, simple ‘transposition’ of a single IS26 should not be invoked.
Simultaneous Determination of 5- and 6-APB in Blood, Other Body Fluids, Hair and Various Tissues by HPLC--MS-MS
Authors: Hofmann Vanessa , Sundermann Tom Richard , Landmann Aysche , Rechtsteiner Stefanie , Schmitt Georg , Bartel Marc 
Published: February 2021

Abstract
5-(2-Aminopropyl)benzofuran (5-APB) and 6-(2-aminopropyl)benzofuran (6-APB) are benzofuran analogues of amphetamine and belong to the category of new psychoactive substances. Despite already published fatal 5- and 6-APB intoxication after consumption of both substances in most cases, no sensitive method for the simultaneous detection and quantification of these new psychoactive compounds in human blood samples has yet been developed. Therefore, an easy and fast sample preparation and specific high-performance liquid chromatography and tandem mass spectrometry methods for the determination of both substances in blood were established and validated. In a fatal intoxication in 2017 at the Institute of Forensic and Traffic Medicine in Heidelberg, Germany, concentrations of 850 (5-APB) and 300 ng/mL (6-APB) were determined in peripheral blood. Besides, other body fluids (central blood, urine and bile), hair and various tissues were examined to verify the presence of both compounds and to gain first insights into their distribution. In this publication, we show a method for the simultaneous determination of 5- and 6-APB in human samples by a chromatographic method and to investigate their distribution in the human body.
Structural catalog of core Atg proteins opens new era of autophagy research
Authors: Matoba Kazuaki , Noda Nobuo N 
Published: February 2021

Abstract
Autophagy, which is an evolutionarily conserved intracellular degradation system, involves de novo generation of autophagosomes that sequester and deliver diverse cytoplasmic materials to the lysosome for degradation. Autophagosome formation is mediated by approximately 20 core autophagy-related (Atg) proteins, which collaborate to mediate complicated membrane dynamics during autophagy. To elucidate the molecular functions of these Atg proteins in autophagosome formation, many researchers have tried to determine the structures of Atg proteins by using various structural biological methods. Although not sufficient, the basic structural catalog of all core Atg proteins was established. In this review article, we summarize structural biological studies of core Atg proteins, with an emphasis on recently unveiled structures, and describe the mechanistic breakthroughs in autophagy research that have derived from new structural information.
The Influence of Interdependence in Networked Publics Spheres: How Community-Level Interactions Affect the Evolution of Topics in Online Discourse
Authors: Yang Aimei , Choi Ian Myoungsu , Abeliuk Andrés , Saffer Adam 
Published: February 2021

Abstract

Investigations of networked public spheres often examine the structures of online platforms by studying users’ interactions. These works suggest that users’ interactions can lead to cyberbalkanization when interlocutors form homophilous communities that typically have few connections to others with opposing ideologies. Yet, rather than assuming communities are isolated, this study examines community-level interactions to reveal how communities in online discourses are more interdependent than previously theorized. Specifically, we examine how such interactions influence the evolution of topics overtime in source and target communities. Our analysis found that (a) the size of a source community (the community that initiates interactions) and a target community (the community that receives interactions), (b) the stability of the source community, and (c) the volume of mentions from a source community to a target community predicts the level of influence one community has on another’s discussion topics. We argue this has significant theoretical and practical implications.

Lay Summary
Political discussions online, especially those in the United States, seem to range between harmonious discussions of likeminded people and heated debates that end with few, if any, who have changed their minds. Researchers have often examined these balkanized/polarized situations by studying online communities as isolated echo chambers of opinion. Our study focuses on the interactions between online communities who have different worldviews. We examine communities engaged in the global refugee crisis. We consider how the inter-community interactions influence the agenda of the respective communities. Our longitudinal analysis on the one hand confirms previous studies, namely that intra-community interactions indeed resemble echo chambers. On the other hand, we also find that there is interdependence in the inter-community discussion topics, albeit some communities had greater influence on other communities’ discussion topics. For example, larger, more stable communities command more influence.
Navigating Sexual Racism in the Sexual Field: Compensation for and Disavowal of Marginality by Racial Minority Grindr Users in Singapore
Authors: Ang Ming Wei , Tan Justin Ching Keng , Lou Chen 
Published: February 2021

Abstract This study investigates racialized sexual desires of Grindr users in Singapore, a multiracial East Asian society. We found that users are continually pigeonholed into racial categories tethered to stereotypes, hierarchizing users such that the Chinese majority are considered more desirable. Users employ race labels to communicate racial membership, circumnavigating Grindr’s preset ethnic categories. Users also creatively appropriate interface affordances to enforce racialized preferences; this includes a preoccupation with verifying racial identities, especially through photos. Racial minorities strategically respond to sexual racism by negotiating for Chinese majority membership, emphasizing the cosmopolitan self over the ethnic self, and/or reframing the situation to disavow victimhood. This research counterbalances the ethnocentric focus of existing sexual racism literature on white-centric contexts by applying sexual fields theory to multiracial East Asia, yielding meaningful theoretical contributions. We also foreground the importance of considering internal dispositions of feelings and attitudes as situated resistance against sexual racism on Grindr.Lay summaryThis article explores how sexual desires of Singaporean users on Grindr (a gay dating app) are socially conditioned to include racial preferences, which in turn constitutes sexual racism. This research is important as it examines the complexities of sexual racism within a multiracial and postcolonial East Asian context, balancing the existing scholarly focus on sexual racism in Western societies. Our interviews with Grindr users in Singapore revealed that users tend to slot themselves (and others) into racial categories that appear fixed and linked to racial stereotypes. This allows a pecking order to emerge, such that the racial majority (Singaporean Chinese users) are generally seen as most desirable. Race is therefore one important dimension of the interactions on Grindr. If racial identity is not immediately obvious on app profiles, users often seek to find out the racial identities of other potential partners by, for example, requesting photos to make guesses about their race. We also studied the responses of racial minorities to sexual racism. These strategies include trying to present a Chinese or Chinese-mixed racial identity, emphasizing an identity that is globalized rather than ethnic, and reframing their situation to disavow their victimhood.
Insecticide Resistance Monitoring of the Diamondback Moth (Lepidoptera: Plutellidae) Populations in China
Authors: Wang Jing , Zheng Xiaobin , Yuan Jiangjiang , Wang Shuaiyu , Xu Baoyun , Wang Shaoli , Zhang Youjun , Wu Qingjun 
Published: February 2021

Abstract
The diamondback moth, Plutella xylostella L., is a worldwide crop pest that is difficult to control because of its ability to develop resistance to many insecticides. To provide a reference for resistance management of P. xylostella in China, the present study used a leaf-dip bioassay to monitor the resistance of P. xylostella to nine insecticides in eight regions of China. The results showed that P. xylostella had developed a high level of resistance to beta-cypermethrin (resistance ratio [RR] &gt; 112), and moderate (RR &lt; 40) to high levels of resistance to indoxacarb, abamectin, and chlorfluazuron. For chlorantraniliprole, RRs &gt; 100 were found in Midu (Yunnan Province) and Jinghai (Tianjin). In most regions, the resistance to spinetoram and chlorfenapyr and Bacillus thuringiensis (Bt) was low. No resistance was detected to diafenthiuron. Overall, P. xylostella resistance to insecticides was higher in Midu than in other regions. The data in this study should help guide the selection of insecticides for management of P. xylostella in China.
Sex Ratios in a Warming World: Thermal Effects on Sex-Biased Survival, Sex Determination, and Sex Reversal
Authors: Edmands Suzanne 
Published: February 2021

AbstractRising global temperatures threaten to disrupt population sex ratios, which can in turn cause mate shortages, reduce population growth and adaptive potential, and increase extinction risk, particularly when ratios are male biased. Sex ratio distortion can then have cascading effects across other species and even ecosystems. Our understanding of the problem is limited by how often studies measure temperature effects in both sexes. To address this, the current review surveyed 194 published studies of heat tolerance, finding that the majority did not even mention the sex of the individuals used, with &lt;10% reporting results for males and females separately. Although the data are incomplete, this review assessed phylogenetic patterns of thermally induced sex ratio bias for 3 different mechanisms: sex-biased heat tolerance, temperature-dependent sex determination (TSD), and temperature-induced sex reversal. For sex-biased heat tolerance, documented examples span a large taxonomic range including arthropods, chordates, protists, and plants. Here, superior heat tolerance is more common in females than males, but the direction of tolerance appears to be phylogenetically fluid, perhaps due to the large number of contributing factors. For TSD, well-documented examples are limited to reptiles, where high temperature usually favors females, and fishes, where high temperature consistently favors males. For temperature-induced sex reversal, unambiguous cases are again limited to vertebrates, and high temperature usually favors males in fishes and amphibians, with mixed effects in reptiles. There is urgent need for further work on the full taxonomic extent of temperature-induced sex ratio distortion, including joint effects of the multiple contributing mechanisms.
On the utility of the category of ‘Hinduism’ in Thailand
Authors: McGovern Nathan 
Published: February 2021

AbstractAlthough earlier scholarship on Southeast Asia focused on the legacy of Hinduism in that region, the recent turn away from models of syncretism and toward explaining Buddhism in terms of the ‘Pali imaginaire’ has led to a sharp de-emphasis of ‘Hinduism’ as a valid category for understanding religion in Buddhist countries of Southeast Asia such as Thailand. In this article, I argue that ‘Hinduism’ still has a utility for the study of religion in Thailand as long as it is used correctly—that is, not to label something as ‘non-Buddhist’. I argue that we must study Theravāda Buddhism and the Pali imaginaire as embedded in a broader world context that includes Hinduism within it. In particular, certain historical continuities between the Sanskrit Cosmopolis that once reigned over Southern Asia and the Pali Cosmpolis that formed in Sri Lanka and Southeast Asia after the former's collapse have resulted in a space for Hinduism to have ongoing agency within Pali Buddhism.