bluuebunny/crossref_metadata_2025_split huggingface dataset:
Age is A Critical Factor In Wrist Function After Surgical Therapy of Patients Suffering From A-Type Distal Radius Fracture: A PROM Study
Authors: Von Matthey F , Weber Johannes , Müller Michael , Biberthaler Peter , Abel Helen 
Published: December 2021

Abstract
Background: Although distal radius fractures (DRF) are the most common fractures of the human body there is still no consensus concerning the best treatment option, especially for type A fractures. Moreover, studies concerning other anatomic regions could prove that patient age is of high impact on therapy and outcome. Therefore, we have quantified wrist function within a retrospective study design using PROM and we have analysed the influence of age between control and patient collective and young versus old, respectively.Patients and Methods: The retrospective study included all patients with a surgically treated DRF type A and a control group of healthy patients, age and gender matched. The function of the wrist was examined with a self-assessment questionnaire the Munich Wrist Questionnaire (MWQ) according to the patient related outcome measurements (PROM). Results: PROM was answered by 110 patients with DRF type A. The average follow-up was 66 months. 110 healthy wrists were the control group and subgroup matching induced similar age group distribution. Hence, 7 individuals < 30 years, 67 between 31 and 64 years, 29 control individuals between 65 and 79 years and 7 control individuals > 80 years, respectively. Women were significantly older than the men (59±15 vs. 47±17).In overall analysis, there was no significant difference between control and patient group (96±6 vs. 95±7). Function was significantly different between control and patient group < 30 years (100±1 vs. 98±2). In the control group the function was significantly decreasing with advanced aging whereas in the patient group this influence was absent. The difference between age group <30 and 65-79 and >80 and between 30-64 and 65-79 and >80 was significantly different with increasing age. Conclusion: PROM is a suitable tool for a retrospective study design as numerous patients can be analysed. The influence of age is critical for wrist function. Hence, we therefore strongly suggest that this information should be taken into consideration for future study plans.
Non-Fasting Changes of Hs-CRP Level in Chinese Patients With Coronary Heart Disease After A Daily Meal
Authors: Liu Ling , Lin Qiu-Zhen , Zang Xue-Yan , Fu Yan , Wen Xingyu , Liu Qi-Ming 
Published: December 2021

Abstract
High-sensitivity C-reactive protein (hs-CRP) is a key inflammatory factor in atherosclerotic cardiovascular diseases. In Chinese patients with coronary heart disease (CHD), the changes in hs-CRP levels after a daily meal and the effect of statins on those were never explored. A total of 300 inpatients with CHD were included. Hs-CRP levels were measured in fasting and non-fasting state at 2 hour (h) and 4h after a daily breakfast. Group with fasting hs-CRP ≤ 3mg/L had significantly higher percentage of patients with statins using ≥ 1 month (m) than that with fasting hs-CRP > 3mg/L (51.4% vs. 23.9%, P < 0.05). Hs-CRP levels were significantly higher in non-fasting state (P < 0.05). Interestingly, the hs-CRP didn’t elevate significantly in inpatients with statins using ≥ 1m in hs-CRP > 3mg/L group, but it elevated significantly after meal in inpatients without and with statins using < 1m (P < 0.05). About 32% of patients with non-fasting hs-CRP > 3mg/L came from those with fasting hs-CRP ≤ 3mg/L. In conclusion, hs-CRP levels increased significantly in CHD patients after a daily meal. When fasting hs-CRP > 3mg/L but not ≤ 3mg/L, statins work partly in reducing hs-CRP elevation in non-fasting state.
Current Causes of Death in Familial Hypercholesterolemia
Authors: Benedí Victoria Marco , Bea Ana M , Cenarro Ana , Jarauta Estíbaliz , Laclaustra Martín , Civeira Fernando 
Published: December 2021

Abstract
Background: Familial hypercholesterolemia (FH) is a codominant autosomal disease characterized by high low-density lipoprotein cholesterol (LDLc) and high risk of premature cardiovascular disease (CVD). The molecular bases have been well defined and effective lipid-lowering is possible. This analysis aimed to study the current major causes of death of genetically defined heFH. Methods: Case-control study designed to analyze life-long mortality in a group of heFH and control families. Data of first-degree family members of cases and controls (non-consanguineous cohabitants), including deceased relatives, were collected from a questionnaire and review of medical records. Mortality was compared among heFH, non-heFH, and non-consanguineous family members.Results: We analyzed 813 family members, 26.4% of them, deceased. Among deceased, mean age of death was 69.3 years in heFH, 73.5 years in non-heFH, and 73.2 years in non-consanguineous, differences that were not statistically significant. Among them, CVD cause of death was 59.7% in heFH, 37.7.% in non-heFH, and 37.4% in non-consanguineous (P=0.012). These differences were greater restricting the analyses to parents’ mortality. The hazard ratio of dying from CVD was 3.02 times higher (95% CI, 1.90-4.79) in heFH members in comparison with the other two groups (non-FH and non-consanguineous), who did not differ in their risk.Conclusions: Current CVD mortality in heFH is lower and occurs later than that described in the last century but still higher than in non-FH. This better prognosis in CVD risk is not associated with changes in non-CVD mortality.
Development of Monoclonal Antibody Based IgG and IgM ELISA for Diagnosis of Severe Fever with Thrombocytopenia Syndrome Virus Infection
Authors: Zhang Mei , Du Yanhua , Yang Li , Zhan Lin , Yang Bin , Huang Xueyong , Xu Bianli , Morita Koichi , Yu Fuxun 
Published: December 2021

Abstract

Background: Severe fever with thrombocytopenia syndrome virus (SFTSV) is a newly emerged virus that possesses a great threat to human health because of the high fatality rate. Method: To develop sensitive and specific sero-diagnosis systems for SFTSV infections, monoclonal antibodies (MAbs) against recombinant SFTSV nucleocapsid (rSFTSV-N) protein were developed by immunizing BALB/C mice with rSFTSV-N protein and fusing the spleen cells with SP2/0 myeloma cells. Three hybridoma cell lines secreting MAbs against rSFTSV-N were obtained. MAb based IgG sandwich enzyme linked immunosorbent assay (ELISA) and IgM capture ELISA systems were established by using the newly developed MAbs. One hundred fifteen clinical suspected SFTS patient serum samples were used to evaluate the newly established systems by comparing with the total antibody detecting sandwich ELISA system and indirect ELISA systems. Results: The MAb based sandwich IgG ELISA was perfectly matched with that of the total antibody sandwich ELISA and the indirect IgG ELISA with a sensitivity and specificity of 100%. IgM capture ELISA results perfectly matched with that of the total antibody sandwich ELISA while was more sensitive comparing with the indirect IgM ELISA. Conclusions: The MAbs against rSFTSV-N protein offer new tools for SFTSV studies and our newly developed MAb-based IgG and IgM capture ELISA systems would offer safe and useful tools for diagnosis of SFTS virus infections and epidemiological investigations.
Nationwide Comprehensive Epidemiological Study of Rare Diseases in Japan Using a Health Insurance Claims Database
Authors: Ninomiya Kota , Okura Masahiro 
Published: December 2021

Abstract
BackgroundMore than 7,000 diseases constitute what are called rare diseases, and they mostly have no specific treatment. Disease profiles, such as prevalence and natural history, among the population of a specific country are essential in determining for which disease to research and develop drugs. In Japan, disease profiles of fewer than 2,000 rare diseases, called Nanbyo, have been investigated. However, non-Nanbyo rare diseases remain largely uninvestigated. Accordingly, we reveal the prevalence and natural history of rare diseases among the Japanese population, using the National Database of Health Insurance Claims and Specific Health Checkups of Japan, which covered 99.9% of public health insurance claims from hospitals and 97.9% from clinics as of May 2015. Then, we compared them with the data reported in Orphanet. This cross-disease study is the first to analyze rare-disease epidemiology in Japan with high accuracy, disease coverage, and granularity.ResultsWe were provided with the number of patients of approximately 4,500 rare diseases by sex and age for 10 years with the permission of the Ministry of Health, Labour and Welfare. About 3,000 diseases have equivalent terms in Orphanet and other medical databases. The data show that even if the Nanbyo systems do not cover a rare disease, its patients survive in many cases. Moreover, regarding natural history, genetic diseases tend to be diagnosed later in Japan than they are in the West. The data collected for this research work are available in the supplement and the website of NanbyoData.ConclusionsOur research work revealed the basic epidemiology and the natural history of Japanese patients with rare diseases using a health insurance claims database. The results imply that the coverage of the present Nanbyo systems is inadequate for rare diseases. Therefore, fundamental reform might be needed to reduce unfairness between rare diseases. Moreover, most diseases in Japan follow a tendency similar to those reported in Orphanet. However, some diseases are detected later, partly because fewer clinical genetic tests are available in Japan than there are in the West. Finally, we hope that our data and analysis accelerate drug discovery for rare diseases in Japan.
Analysis The Effect of Gene and Platelet Testing Guide Dual Anti-Upgrade Therapy After PCI in Patients With ACS
Authors: Guo Guofeng 
Published: December 2021

Abstract
Objective

To analyze whether CYP2C19 gene and platelet testing guide ACS patients PCI benefit from postoperative dual antiplatelet escalation therapy.
Methods

Selecting ACS patients with 209 routine PCI surgery from January 2018 to January 2019 in Department of Cardiology, Third Affiliated Hospital of Guangzhou Medical University. Preoperative administration of aspirin 300mg and clopidogrel 600mg, and continued administration of clopidogrel 75mg/d and aspirin 100mg/d after operation. Genotype and light transmittance aggregation (LTA) was detected by gene chip 24 h after operation. According to genotype the remaining patients were divided into non loss of function (Non-LOF) alleles group Extensive-metabolisms (EMs) type, loss of function (LOF) alleles group as Intermediate metabolic (IMs) type and Poor-metabolisms (PMs) type. Define the maximum platelet aggregation rate (MPA)≥46% as hyperplatelet reactivity (HPR). The LOF group consisted of 23 patients who had both HPR and proclopidogrel and were upgraded to tigrillo for further treatment.The remaining patients without HPR who continued to be treated with clopidogrel comprised 90 patients in the LOF group without upgrade and 95 patients in the non-LOF group who continued to be treated with clopidogrel.Major adverse cardiovascular events (MACE) were recorded in the follow-up period of 1 year, and the incidence of MACE in the three groups was compared to determine whether gene and platelet detection could guide the benefit of dual anti-platelet upgrade therapy in ACS patients after PCI.
Results

There were 26 cases occurred during follow-up MACE, among which the incidence of unstable angina recurrence and overall MACE in the LOF allele-not-up group was the highest and significantly different compared with the Non-LOF allele group (P<0.05), there was no significant difference compared with the LOF allele-up group (0.05). while there was no significant difference between the Non-LOF allele and the LOF allele upgrading group (P>0.05).
Conclusions

Gene is an important factor in the difference of platelet reactivity and is associated with MACE. Upgraded treatments for high-risk patients screened for gene and platelet testing did not benefit.
Numerical Evaluation of The Nonlinear Behavior of Soil-Pile Interaction Under The Effect of Coupled Static-Dynamic Loads
Authors: Al-Jeznawi Duaa , Jais ISMACAHYADI Mohamed , Albusoda Bushra S. 
Published: December 2021

Abstract
Liquefaction of saturated soil layers is one of the most common causes of structural failure during earthquakes. Liquefaction occurs as a result of increasing pore water pressure, whereby the rise in water pressure occurs due to unexpected change in stress state under short-term loading, i.e., shaking during an earthquake. Thus, general failure occurs when the soil softens and eliminates its stiffness against the uplift pressure from the stability of the subsurface structure. In this case, the condition of soil strata is considered undrained because there is not enough time for the excess pore water pressure to dissipate when a sudden load is applied. To represent the non-linear characteristics of saturated sand under seismic motions in Kobe and Ali Algharbi earthquakes, the computational model was simulated using the UBCSAND model. The current study was carried out by adopting three-dimensional-based finite element models that were evaluated by shaking table tests of a single pile model erected in the saturated soil layers. The experimental data were utilized to estimate the liquefaction and seismicity of soil deposits. According to the results obtained from the physical models and simulations, this proposed model accurately simulates the liquefaction phenomenon and soil-pile response. However, there are some differences between the experiment and the computational analyses. Nonetheless, the results showed good agreement with the general trend in terms of deformation, acceleration, and liquefaction ratio. Moreover, the displacement of liquefied soil around the pile was captured by the directions of vectors generated by numerical analysis, which resembled a worldwide circular flow pattern. The results revealed that during the dynamic excitation, increased pore water pressure and subsequent liquefaction caused a significant reduction in pile frictional resistance. Despite this, positive frictional resistance was noticed through the loose sand layer (near the ground surface) until the soil softened completely. It is worth mentioning that the pile exhibited excessive settlement which may attribute to the considerable reduction, in the end, bearing forces which in turn mobilizing extra end resistance.
Association of Glutathione S-transferases (GSTT1, GSTM1 and GSTP1) Genes Polymorphisms with Nonalcoholic Fatty Liver Disease Susceptibility: A Meta-analysis of Case-control Studies
Authors: Zhu Yi , Qiao Ming 
Published: December 2021

Abstract
Background: Glutathione S-transferases (GSTs) genes single-nucleotide polymorphisms (SNPs) have been connected with the susceptibility of nonalcoholic fatty liver disease (NAFLD), but with inconsistent results across the current evidences. The present work was schemed to explore the association between GSTs genes polymorphisms and the NAFLD vulnerability via meta-analysis.Methods: PubMed, Web of Science, Cochrane Library, China National Knowledge Infrastructure (CNKI) and Wanfang were retrieved for eligible literatures previous to March 10, 2021. The odds ratio (OR) of the dichotomic variables and the standardized mean difference of quantitative variables with corresponding 95% confidence intervals (95%CIs) were computed to evaluate the strength of the associations. The quality of included studies were assessed via using Newcastle-Ottawa Scale (NOS).Results: In total, 7 case-control studies encompassing 804 NAFLD patients and 1362 disease-free controls in this meta-analysis. Ultimately, this analysis included six, five and five studies for GSTM1, GSTT1 and GSTP1 polymorphisms respectively. The pooled data revealed that the GSTs genes single-nucleotide polymorphisms had conspicuous associations with NAFLD susceptibility: for GSTM1, null vs. present, OR=1.46, 95%CI 1.20-1.79, P=0.0002; for GSTT1, null vs. present, OR=1.34, 95%CI 1.06-1.68, P=0.01; for GSTP1, Ile/Val or Val/Val vs. Ile/Ile, OR=1.60, 95%CI 1.23-2.09, P=0.0005.Conclusion: This work revealed that the GSTM1 null, GSTT1 null and GSTP1-Val genotypes might be related to increased NAFLD susceptibility.
Enhanced Recovery After Surgery Pathway: Association with Lower Incidence of Wound Complications and Severe Hypoalbuminemia in Patients Undergoing Posterior Lumbar Fusion Surgery
Authors: Wang Shuaikang , Wang Peng , Li Xiangyu , Sun Wenzhi , Kong Chao , Lu Shibao 
Published: December 2021

Abstract
Background. Wound complications were associated with worse satisfaction and additional costs in patients underwent posterior lumbar fusion(PLF) surgery ,the relationship between enhanced recovery after surgery(ERAS)pathway and wound complications remains poorly characterized.Methods. In this retrospective single-center study, we compared 530 patients receiving ERAS pathway care with previous 530 patients in non-ERAS group. The primary aim of our study was to identify the relationship between ERAS program and the incidence of postoperative wound related complications and other complications following PLF surgery, other outcomes included the length of stay(LOS), 90-day hospital and rehabilitation center readmission.Results. Average patient age was 65yr. There were more patients with old cerebral infarction in ERAS group (p<0.01), other demographics and comorbidities were similar between both groups. Patients in ERAS group had a lower incidence of postoperative wound-related complications compared with non-ERAS group(12.4 vs 17.8%, p=0.02).The non-ERAS group had a significantly higher rate of wound dehiscence or poor wound healing(6% vs 3%, p=0.02). ERAS group had a lower incidence of server postoperative hypoalbuminemia(serum albumin less than 30g/L)(15.8% vs 9.0% p<0.01).Additionally, ERAS patients had a shorter postoperative LOS (8.0±1.5 vs 9.5±1.7 p<0.01), lower rate of readmission within 90 days (1.9% vs 6.4%, p<0.01) and discharge to rehabilitation center (4.2% vs 1.0%, p<0.01).Conclusion. ERAS pathway may help to decrease the rates of postoperative wound complications and server hypoalbuminemia following PLF surgery, additionally, we demonstrated that ERAS pathway was also associated with shorter LOS and lower rate of readmissions within 90-day.
Ringed Fluorodeoxyglucose Uptake Predicted Poor Prognosis After Resection of Pulmonary Pleomorphic Carcinoma
Authors: Shishido Yutaka , Aoyama Akihiro , Hara Shigeo , Sato Yuki , Tomii Keisuke , Hamakawa Hiroshi , Takahashi Yutaka 
Published: December 2021

Abstract
Background:Pulmonary pleomorphic carcinoma (PPC) is a relatively rare and poorly differentiated non-small cell carcinoma. This study aimed to investigate the clinicopathological features including programmed cell death ligand 1 (PD-L1) expression status in patients with PPC who underwent curative resection.Methods:We retrospectively studied 29 consecutive patients who had undergone anatomical lung resections for PPC. Perioperative and pathological variables, including radiological findings, were investigated to define prognostic factors.Results:Overall survival (OS) rates were 71.8% at 1 year and 60.0% at 5 years. Disease-free survival (DFS) rates were 54.8% at 1 year and 43.6% at 5 years. Univariate analysis revealed that ringed fluorodeoxyglucose (FDG) uptake on positron emission tomography/computed tomography (PET/CT) (p=0.003), a cavity in the tumor on CT (p=0.004), and tumor size (>40mm) (p=0.014) were poor prognostic factors for OS. Regarding DFS, ringed FDG uptake (p=0.002), a cavity on CT (p<0.001), tumor size (p=0.007), and pleural invasion (p=0.014) were poor prognostic factors. PD-L1 expression was not a prognostic factor. Conclusion:Early relapse was frequently observed. This study showed for the first time that ringed FDG uptake on PET/CT is a poor prognostic factor of PPC. PD-L1 expression status was not related to the prognosis. Trial registration:The study was approved by the Kobe City Medical Center General Hospital’s ethics board (No. 20112) on August 20, 2020.
LncRNA MBNL1-AS1 Represses Proliferation and Cancer Stem-Like Properties of Breast Cancer Through MBNL1-AS1/ZFP36/CENPA Axis
Authors: Ding Yu , Li Yingjie , Duan Yunqiang , Wang Wan , Zheng Wei , Cheng Weilun , Qi Yuan , Feng Jianyuan , Chen Ziang , Yu Tianshui , Hu Anbang , Ma Fei , Guo Baoliang 
Published: December 2021

Abstract
Background: Emerging studies suggested the notion that long noncoding RNAs (lncRNAs) were key regulators of cancer progression. In this research, the expression and roles of MBNL1-AS1 were explored in breast cancer (BC).Methods: In the present research, the MBNL1-AS1 expression in breast cancer tissue, as well as in cell line was studied by qRT-PCR assays. The effects of MBNL1-AS1 on proliferation and stemness were evaluated by MTT assays, colony formation assays, orthotopic breast tumor mice models, and sphere formation assays. Flexmap 3D assays were performed to show that MBNL1-AS1 downregulated the Centromere protein A (CENPA) secretion in BC cells. Western blot, RNA pull-down assays, RNA immunoprecipitation (RIP) assays, and Fluorescence in situ hybridization (FISH) were conducted to detect the mechanism.Results: The results revealed that the expression levels of MBNL1-AS1 were downregulated in breast cancer tissues and cell lines. In vitro and in vivo studies demonstrated that overexpression of MBNL1-AS1 markedly inhibited BC cells proliferation and stemness. RNA pull-down assay, RIP assay, western blot assay, and qRT-PCR assay showed that MBNL1-AS1 downregulated CENPA mRNA via directly interacting with Zinc Finger Protein 36 (ZFP36) and subsequently decreased the stability of CENPA mRNA. Restoration assays also confirmed that MBNL1-AS1 suppressed the CENPA-mediated proliferation and stemness in breast cancer cells. Conclusions: We elucidated a new mechanism for how MBNL1-AS1 regulated the phenotype of BC and targeting the MBNL1-AS1/ZFP36/CENPA axis might serve as therapeutic targets for BC patients.
A Dynamic Approach for Early Risk Prediction of Gram-Negative Bloodstream Infection and Systemic Inflammatory Response Syndrome in Febrile Pediatric Hemato-Oncology Patients
Authors: Rubio José Antonio Villegas , Moraleda Pilar Palomo , Delgado Ana de Lucio , Sánchez Gonzalo Solís , García Belén Prieto , Galán Corsino Rey 
Published: December 2021

Abstract
The aim of this paper was to evaluate the usefulness of C-Reactive Protein (CRP), Procalcitonin (PCT) and Interleukine 6 (IL6) biomarkers in predicting the existence of Gram negative bloodstream infections (Gr-BSI) or the development of Systemic Inflammatory Response Syndrome (SIRS) during the first 24 hours of fever in pediatric cancer patients. The present study included a total of 103 consecutive fever episodes in 44 hemato-oncological pediatric patients, from whom samples for biomarkers CRP, PCT and IL6 were taken upon initial evaluation and then between 12 and 24 hours after.An IL6 value at the first evaluation (IL6-1) higher than 164 pg/ml and an increase in CRP higher than 291% between the first and subsequent samples (CRP-2vs1) showed a statistically significant OR of 26.03 and 19.62, respectively, in multivariate analysis.Conclusion: IL6-1 and CRP-2vs1 showed a strong, independent correlation with Gr-BSI and SIRS episodes and, therefore, could be used as reliable predictors of these kinds of severe episodes. The approach taken in our study, using biomarker variations over time as a variable, has shown itself to be an improvement in the predictive model.