bluuebunny/crossref_metadata_2025_split huggingface dataset:
Germinal GLT8D1, GATAD2A and SLC25A39 mutations in a patient with a glomangiopericytal tumor and five different sarcomas over a 10-year period
Authors: Beddok Arnaud , Perot Gaëlle , Guellec Sophie Le , Thebault Noemie , Coutte Alexandre , Sevestre Henri , Chauffert Bruno , Chibon Frédéric 
Published: February 2021

Abstract
Soft tissue sarcoma represents about 1% of all adult cancers. Occurrence of multiple sarcomas in a same individual cannot be fortuitous. A 72-year-old patient had between 2007 and 2016 a glomangiopericytal tumor of the right forearm and a succession of sarcomas of the extremities: a leiomyosarcoma of the left buttock, a myxofibrosarcoma (MFS) of the right forearm, an MFS of the left scapula, a left latero-thoracic MFS and two undifferentiated sarcomas on the left forearm. Pathological examination of the six locations was not in favor of disease with local/distant recurrences but could not confirm different diseases. An extensive molecular analysis including DNA-array, RNA-sequencing and DNA-Sanger-sequencing, was thus performed to determine the link between them. The genomic profile of the glomangiopericytal tumor and the six sarcomas revealed that five sarcomas were different diseases and one was the local recurrence of the glomangiopericytal tumor. While the chromosomal alterations in the six tumors were different, a common somatic CDKN2A/CDKN2B deletion was identified. RNA-sequencing of five tumors identified mutations in GLT8D1, GATAD2A and SLC25A39 in all samples. The germline origin of these mutations was confirmed by Sanger-sequencing. Innovative molecular analysis methods have made possible a better understanding of the complex tumorigenesis of multiple sarcomas.
Implementation of respectful maternal care in health facilities in Conakry, Guinea
Authors: Momo1 Soumah Aboubacar  Fode , Cellou1 Diallo Mamadou , Ibrahima1 Conte , Ibrahima1 Sylla , Hawa2 Bah Oumou , Kadiatou2 Diallo Abdourahamane , Koussy1 Bah Ibrahima , Diallo1 Abdourahamane , Telly1 Sy , Namory2 Keita 
Published: February 2021

Abstract
Objective: Describe the implementation of Respectful Maternal Care in health facilities in the capital and discuss lessons learned. Methods: This was a cross-sectional descriptive study by direct observation of providers from November 29 to December 01, 2020. It consisted of a baseline assessment of pilot sites prior to training in respectful maternal care, followed by the traditional five-day training and an extension to urban health centers, due to their on-site training in SONUB (obstetric and neo-natal care) Basic emergency natals) including the Respectful Maternal Care Training Module (PMF). Random random sampling by random draw, three guards per site, a midwife with a parturiente and their families, providers of health centers in post-training follow-up, were observed through the seven (7) performance standards (Table2) on the skills received. The investigators were clinical synthesis students who were trained in the use of the tool. Results: 199 providers (138 midwives, 49 nurses and 12 doctors) were trained in PMSC in the 24 health facilities in the capital. Post-training follow-up through providers made the following findings in the different areas of PMSCs: The seven (7) standards were observed prior to training in the 6 pilot sites (Center Médical Communal (CMC) of Matam, Minière, Ratoma, Coléah, Bernard Kouchner and the maternity hospital of the National Ignace Deen Hospital) (Fig.1), two were achieved or 0.3 the practice improved compared to the situation of the basic evaluation: in the seven areas of respectful maternal care evaluated and monitored, performance varied by area from 26% to 100% (Fig.2). In health centers, good practice was observed with the observation of skills during post-training follow-up: In the seven areas monitored, performance varied from 50% to 100% (Fig.3) Conclusion: SMR. integration was effective in 25 health facilities in the city of Conakry; training, internal and external regular supervision and provider awareness are essential to sustain PMSCs in daily practice.
Human hepatocyte PNPLA3 148M exacerbates rapid non-alcoholic fatty liver disease development in chimeric mice
Authors: Kabbani Mohammad , Michailidis Eleftherios , Steensels Sandra , Fulmer Clifton , Luna Joseph , Pen Jérémie Le , Tardelli Matteo , Razooky Brandon , Ricardo-Lax Inna , Zou Chenhui , Zeck Briana , Stenzel Ansgar , Quirk Corrine , Foquet Lander , Ashbrook Alison , Schneider William , Belkaya Serkan , Lalazar Gadi , Liang Yupu , Pittman Meredith , Devisscher Lindsey , Suemizu Hiroshi , Theise Neil , Chiriboga Luis , Cohen David , Copenhaver Robert , Grompe Markus , Meuleman Philip , Ersoy Baran , Rice Charles , Jong Ype de 
Published: February 2021

Abstract
Advanced non-alcoholic fatty liver disease (NAFLD) is a rapidly emerging global health problem associated with pre-disposing genetic polymorphisms, most strikingly an isoleucine to methionine substitution in patatin-like phospholipase domain-containing protein 3 (PNPLA3-I148M). Here, we study how human hepatocytes with PNPLA3 148I and 148M variants engrafted in the livers of chimeric mice respond to hypercaloric diets. As early as 4 weeks, mice developed dyslipidemia, impaired glucose tolerance, and steatohepatitis selectively in the human graft, followed by pericellular fibrosis after 8 weeks of hypercaloric feeding. The PNPLA3 148M variant, either from a homozygous 148M human donor or overexpressed in a homozygous 148I donor background, caused microvesicular and more severe steatosis. In these livers hepatocytes displayed frequent ballooning degeneration, resulting in more active steatohepatitis than in 148I livers. We conclude that PNPLA3 148M in human hepatocytes exacerbates NAFLD. These models will facilitate mechanistic studies into human genetics associated with advanced fatty liver diseases.
Effects of Comprehensive Psychological and Behavioral Intervention on Mental Symptoms, Quality of Life, and Social Function of Patients During The First Episode of Schizophrenia
Authors: Zhang Yuebing , Yin Zhoumin , Shang Luning 
Published: February 2021

Abstract
To analyze the effects of comprehensive psychological and behavioral intervention on mental symptoms, quality of life (QOL) and social function (SF) of patients during the first episode of schizophrenia. A total of 97 patients having the first episode of schizophrenia and who were admitted to our hospital were selected, retrospectively analyzed for clinical materials, and divided into 2 groups based on the intervention mode. The control group (CG) was routinely managed according to the psychiatric practices, whereas the observation group (OG), in accordance with the CG, was managed with enhanced comprehensive psychological and behavioral interventions. The scores of Positive and Negative Syndrome Scale (PANSS), Social Disability Screening Schedule (SDSS), Satisfaction with Life Scale (SWLS), Global Assessment Scale (GAS), Brief Psychiatric Rating Scale (BPRS), and disease recurrence at pre- and post-intervention were compared between the two groups. At post-intervention, scores in general psychopathological, positive, and negative symptoms, total score in PANSS, SDSS, SWLS, GAS, and BPRS were lower in the OG than that in the CG (P<0.05). Further, the recurrence rate was 2.04% in the OG and 25.00% in the CG, with statistically significant difference (P<0.05). The implementation of comprehensive psychological and behavioral interventions in patients having the first episode of schizophrenia is advantageous to improve the mental symptoms, QOL, and SF and reduce the recurrence rate. Therefore, this method deserves popularization.
Use of Shockwave intravascular lithotripsy for the treatment of symptomatic and strongly calcified superior mesenteric artery stenosis
Authors: Arregui Oscar Balboa , Pose Carmen Seoane , Alonso Maria Balboa , Pampín Teresa Bolaño 
Published: February 2021

Abstract
Background: We present the use of intravascular lithotripsy for the definitive treatment of highly calcified superior mesenteric artery stenosis.Case presentation: A 67-year-old diabetic man had chronic postprandial abdominal pain and weight loss. Computed tomography angiography revealed highly calcified stenosis of the superior mesenteric artery. Selective angiography confirmed severe stenosis. A Shockwave lithotripsy balloon catheter was successfully used via brachial access to modify calcified plaque and increase vascular lumen. After twelve months of follow-up the patient had gained weight and had no abdominal postprandial pain.Conclusion: Intravascular lithotripsy could be considered a new treatment modality to modify calcified lesions in the visceral arteries. More controlled studies are needed to demonstrate the efficacy, safety and feasibility of this new technology.Level of Evidence: 4, Case Series
Impact of rainfall variability on soybean yields in Southern Brazil
Authors: Pilau Felipe Gustavo , Marin Fabio Ricardo , Grubert Daniel , Dalmago Genei Antonio 
Published: February 2021

Abstract
As the third soybean-producer state in Brazil, Rio Grande do Sul (RS) presents a known year-on-year unevenness for soybean production, mainly due to water availability. This study aimed to assess the weather effects, with special focus on rainfall during 25 soybean growing seasons and 11 producing regions around the State. Sites were divided into three Clusters according to soybean yield and the effect of El Niño Southern Oscillation (ENSO) was considered in association with soil water balance. Neutral ENSO phases occurred in 32% of the years, while El Niño and La Ninã occurring in 36% and 32% of the years, respectively. Seasons under El Niño normally present higher accumulated rainfall, whereas those under La Niña present a reduction. Data from neutral-year sites of Clusters B and C seems to be more disturbed. No season had statistical difference of rainfall among Clusters under Neutral conditions. In addition, thermal gradient in RS from October to January benefited sites of Cluster A. Interaction of soils with higher water-storage capacity and cooler temperature reduces the water consumption by soybeans, causing lower values of water deficiency. A boundary function relating soybean yield and rainfall displays the limit of 800 mm for significant yield increments, and such amounts of rainfall were only achieved in El Niño seasons. The combined effect of rainfall and soil type on soybean yield, represented by the actual soybean yields-water deficit relationship, led to water propitiate from -3.7 to -15.2 kg mm-1 ha-1. Decision-making on public policies and investments on the soybean industry can be supported from our results, either to better planning the investments on the soybean farming systems depending on the ENSO phase predictions, either to reduce the production risks in the region inherent to local weather.
Experimental and Theoretical Approach of Evaluating Chitosan Ferulic Acid Amide As An Effective Corrosion Inhibitor
Authors: S.J Hepziba Magie Jessima , Subhashini S. , Berisha Avni , Mehmeti Valbonë , Srikan Subramanian Sathy 
Published: February 2021

Abstract
Phenolic acid grafted chitosan has widespread drug delivery applications, as bio adsorbent, packing material, etc., due to its excellent antioxidant and antimicrobial properties. However, for the first time, the anticorrosive efficiency of ferulic acid modified chitosan has been investigated. The prepared chitosan derivative is characterized using spectral methods, thermal analytical methods, surface charge, and particle size analysis. The evaluation of corrosion inhibition potential showed a highest value of 95.96 % at 303 K. Thermodynamic activation and adsorption parameters endorse a mixed adsorption process involving an initial electrostatic interaction followed by chemisorption. Electrochemical studies gave results which agreed well with the gravimetric studies. Surface morphological studies were performed using contact angle measurements, FESEM, EDAX, AFM, optical profilometric and UV spectral techniques. Computational studies involving quantum chemical calculations, Monte Carlo and molecular dynamic simulation studies, and radial distribution function analysis are further done to validate the experimental results.
Development of 30 SNP Markers for the Daphnia Magna based on Restriction Site-associated DNA Sequencing (RAD-seq)
Authors: Zhang Shengman , He Wenhui , Zheng Haisu , Xiong Yiran , Tan Meng , Han Zheng , Chen Siwei , He Peimin 
Published: February 2021

Abstract
Daphnia magna belongs to the Cladocera and plays an important role in the water ecosystem. With the intensification of water pollution, the wild population of D. magna has declined rapidly in recent years, and insufficient molecular markers have limited effective research and conservation of this species. In this research, 30 novel single nucleotide polymorphism (SNP) markers were developed in a cultivar of Daphnia magna and 12 wild Daphnia magna using restriction site-associated DNA sequencing (RAD-seq). The minor allele frequency, observed heterozygosity, and expected heterozygosity ranged from 0.115 to 0.721, 0.073 to 0.800, and 0.077 to 0.520, respectively. The PIC ranged from 0.071 to 0.403. Six loci showed significant deviations from the Hardy-Weinberg equilibrium after Bonferroni correction (p < 0.05).These newly developed polymorphic SNP markers for D. magna are of great significance in terms of the genetic breeding of D. magna, identification of wild and artificially domesticated species and conservation genetics research.
Biodegradation Mechanism of Anionic Polyacrylamide in Coal Preparation Using Molecular Simulation
Authors: Wang Fanglue , Zhang Dongchen , Wu Xuefeng , Deng Shengsong 
Published: February 2021

Abstract
Biodegradation of anionic polyacrylamide (HPAM) and polyacrylate (PAA) by key enzymes, such as amidase and bacterial laccase, have been reported. However, the interaction mechanism between HPAM or PAA and enzymes is still poorly unclear. Here, docking study was undertook to demonstrate the binding modes and interaction details for degradation of HPAM or PAA. Then, bioactivities between PAA and HPAM were compared with frontier orbital theory. The docking results showed that HPAM completely buried in pocket of Rhodococcus sp. N-771 amidase (Rh Amidase), while most of PAA molecule exposed outside pocket of Bacillus subtilis laccase ( B. subtilis laccase ), further suggesting PAA was much more difficult to degrade than HPAM. Hydrophobic interactions and hydrogen bonds were necessary for stabilizing HPAM-Rh Amidase or PAA- B. subtilis laccase complex. The frontier orbital analysis indicated that bioactivity of PAA was higher than that of PAA. These findings provide an insight into enzyme-catalyzed degradation of HPAM. It is helpful in designing highly efficient enzymes against HPAM or PAA to protect environment.
Bioutilization of Chicken Feather Wastes by Newly Isolated Keratinolytic Bacteria Into Protein Hydrolysates With Improved Functionalities
Authors: Prajapati Saugat , Koirala Sushil , Anal Anil Kumar 
Published: February 2021

Abstract
In this study, a novel feather-degrading bacteria B. amyloliquefaciens KB1 was isolated from chicken farm bed (CFB), identified by morphological, physico-biochemical tests followed by 16s rDNA analysis. Among observed isolates, bacterial isolate (KB1) showed the highest degree of feather degradation (74.78 ± 2.94 %) and total soluble protein (205 ± 0.03 mg/ g). Using the same species of bacteria, the optimum fermentation condition was found at 40 oC, pH 9, and 1 % (w/v) feather concentration that produced 260 mg/ g of soluble protein and 86.16 % feather degradation using response surface methodology in a Box-Behnken design space. The obtained hydrolysates exhibited bioactive properties. The amino acid profile showed the increase in concentration of essential amino acid compared with feather meal broth. The selection of safe screening source of this new bacteria in CFB produced hydrolysates with enhanced bioactivity applicable for food, feed, and cosmetic applications along with environmental remediation.
Ursolic Acid Inhibits the Activation of Kupffer Cells by Caspase-11/NLRP3 Inflammasome Signaling Pathways
Authors: Nie Yuan , Huang Chen-kai , Liu Cong , Zhu Xuan 
Published: February 2021

Abstract
Background: Previous studies have indicated that Kupffer cells (KCs) are the main regulatory cells for the activation of hepatic stellate cells (HSCs), and caspase-11/NLRP3 inflammasome signaling plays crucial roles in the activation of monocyte-macrophages. Ursolic acid (UA) is a traditional Chinese medicine with antifibrotic effects, but the molecular mechanism underlying these effects is still unclear.Methods: A mouse primary Kupffer cell line in vitro and liver fibrosis mice (including specific gene knockout mice) in vivo were selected as experimental objects. RT-qPCR and Western blotting techniques were utilized to assess the mRNA and protein expression in each group. ELISA and histological analysis were utilized to assess liver injury and collagen deposition.Results: In vitro, caspase-11/NLRP3 inflammasome signaling promoted the activation of Kupffer cells, and UA inhibited the activation of Kupffer cells by caspase-11/NLRP3 inflammasome signaling. In vivo, UA reversed liver damage and fibrosis in fibrotic mice and was related to Kupffer cells; the expression of Caspase-11/NLRP3 inflammasome signaling in Kupffer cells of the UA group was inhibited. Even in the CCl4 group, the liver damage and fibrosis of NLRP3 knockout mice were alleviated, and related experiments also proved that the inhibitory effect of UA on Kupffer cells was related to the activation of the NLRP3 inflammasome.Conclusion: Caspase-11/NLRP3 inflammasome signal transduction is closely related to the activation of Kupffer cells and the occurrence of liver fibrosis. Additionally, caspase-11/NLRP3 inflammasome signaling serves as a new target for UA antifibrosis treatment.
LncRNA H19 from T-Regulatory-Cell-Derived Exosomes Sponges miR-154-5p to Promote Osteogenic Differentiation of BMMSCs Through Upregulating POSTN
Authors: Jing Zhang , Li Zhigang , Liu Cuicui , Wu Yaxing , Li Chenchen , Jian Meng 
Published: February 2021

Abstract
Background: Mesenchymal stem cells (MSCs) can play an important role in anti-inflammatory function because it can induce Treg cells formation. Recent studies have confirmed that Tregs can release exosomes and induce immune tolerance as important immune cells. Thus, to clarify the function and regulatory mechanism of Treg cells-derived exosomes on osteogenic differentiation of BMMSCs is very important.Methods: CD4+ T cells isolated from mouse spleens were induced into CD4+ Foxp3+ Treg cells and co-cultured with BMMSCs. Then we isolated exosomes derived from Treg cells and tracked the exosomes using fluorescent labeling PKH67.Starbase, miRWalk, targetscan, bioinformatics analysis and functional complementation experiments to find relative lncRNA-miRNAs which involed in osteogenic differentiation of BMMSCs.Results: We found that exosomes derived from Tregs could enter BMMSCs and the expression of osteoblast-related genes were significantly up-regulated compared to the BMMSCs co-cultured with naïve T cells. LncRNA H19 from Tregs-derived exosomes could affect the osteogenic differentiation of BMMSCs in vitro by regulating POSTN expression via sponge miR-154-5p.Conclusions: This study demonstrated that important role of exosomes from Tregs in regulating osteogenic differentiation of BMMSCs. lncRNA H19 from Tregs-derived exosomes plays a positive regulatory role in osteogenic differentiation of BMMSCs through miR-154-5p/POSTN axis. It might has important clinical implications for inflammatory diseases and exosomes released from Tregs could be used as a new type of immunoregulation reagent for the treatment of periodontitis.
Nitrogen-Doped Carbon Spheres-Decorated Graphite Felt as a High-Performance Electrode for Fe Based Redox Flow Batteries
Authors: Dinesh Anarghya , Shankaranarayana Anantha Mylarapattana , Srid Santosh Mysore , Muniswamy Narendra Kumar , Venkatesh Krishna , Muraralidhara H B 
Published: February 2021

Abstract
In this paper, the performance of Fe based redox flow batteries (IRFBs) was dramatically improved by coating N-doped carbon spheres (NDCS) on the graphite felt electrodes. NDCS was synthesized by the single-step hydrothermal method using dextrose and ammonia as a precursor and coated over a graphite felt electrode by electrostatic spraying. The weight of NDCS required for the modification of the electrode to achieve the effective performance of the battery was studied using electrochemical techniques. Cyclic voltammetry (CV) and potentiodynamic polarization study was used to evaluate the kinetic reversibility and linear polarization resistance offered by the electrode towards electrolyte. The characterizing features of the NDCS, untreated graphite felt (UGF) electrode, and optimized modified graphite felt (MGF) electrode were analyzed using SEM, EDAX, XRD, and Raman spectroscopy. The charge-discharge studies were performed for the 132 cm2 IRFB using a 2 mg/cm2 MGF electrode as a positive electrode by varying the current densities from 20 to 60 mA/cm2. The cell resulted in an average coulombic efficiency (CE) of 93%, voltaic efficiency (VE) of 72%, and energy efficiency (EE) of 68% for 15 cycles at the current density of 30 mA/cm2. The improvement in the performance of the IRFB is due to the presence of electrochemically active nitrogen-bearing carbon catalysts. In this paper, the pioneering effort has been made to improve the efficiency of the IRFB with an active area of 132 cm2 using glycine as the ligand.
Integration of Clinical Identifications With Deep Transferrable Imaging Feature Representations Can Help Predict Prostate Cancer Aggressiveness and Outcome
Authors: Bao Jie , Hou ying , Zhi Rui , Wang Ximing , Shi Hai-bin , Hu Chunhong , zhang yudong 
Published: February 2021

Abstract
Objective: To develope a generalizable machine learning platform, designated PI-Risk, which incorporates clinicians’ prior identifications with deep transferrable imaging feature representations into predictive models for PCa Gleason grade. Patients and Methods: A retrospective study included 1442 biopsy-naïve patients from two tertiary care medical centers between January 2014 and December 2019. We investigated an interpretable risk assessment model (PI-Risk) to predict risk stratification of PCa using . The performance of PI-Risk model was independently tested on 232 internal test datasets, and on 539 external validation datasets. Model performance was typically evaluated against a “ground truth” with imaging-histopathologic annotations using receiver operating characteristic (ROC). Detection rates such as true positive, true negative, false positive and false negative rate were reported using a confusion matrix analysis. The Cox model’s performance was evaluated based on Harrell’s concordance index (C-index), calibration curves and Kaplan–Meier survival analysis. Results: The PI-Risk integrated with 10 risk factors is formed to accurate risk stratification. In multinomial regression analyzing model, predicted IntraT-Rad G0 and PI-RADS score 2 were two independent predictors of G0 stage. Predicted IntraT-Rad G1 (OR, 2.42; 95% CIs, 2.13–2.86, p < 0.001) and PSA 4-10 ng/ml were two independent predictors of G1 stage. PSA 10-20 ng/ml, Predicted PeriT-DLR-SqueezeNet G1 and Predicted IntraT-Rad G3 were three independent predictors of G2 stage. PI-RADS score 5 were the independent predictor of G3 stage. PI-RADS score 5 and PSA > 100 ng/ml were two independent predictors of G4 stage. Combined use of PSA 4-10 ng/ml, PI-RADS 1-2 and stacked IntraT-Rad G0-1 resulted in excellent NPV (94.1%) for CIS diseases; and combined use of PSA >100 ng/ml and PI-RADS 5 resulted in high PPV (79.8%) for high-risk PCa. In follow-up, patients stratified by PI-Risk showed significantly different biochemical recurrence rate after surgery.Conclusions: We concluded that the PI-Risk can offer a noninvasive alternative tool to stratify PCa aggressiveness. This enables a step towards PCa risk stratification.