bluuebunny/crossref_metadata_2025_split huggingface dataset:
Regulating coordination number in atomically dispersed Pt species on defect-rich graphene for n-butane dehydrogenation reaction
Authors: Chen Xiaowen , Peng Mi , Cai Xiangbin , Chen Yunlei , Jia Zhimin , Deng Yuchen , Mei Bingbao , Jiang Zheng , Xiao Dequan , Wen Xiaodong , Wang Ning , Liu Hongyang , Ma Ding 
Published: May 2021

AbstractMetal nanoparticle (NP), cluster and isolated metal atom (or single atom, SA) exhibit different catalytic performance in heterogeneous catalysis originating from their distinct nanostructures. To maximize atom efficiency and boost activity for catalysis, the construction of structure–performance relationship provides an effective way at the atomic level. Here, we successfully fabricate fully exposed Pt3 clusters on the defective nanodiamond@graphene (ND@G) by the assistance of atomically dispersed Sn promoters, and correlated the n-butane direct dehydrogenation (DDH) activity with the average coordination number (CN) of Pt-Pt bond in Pt NP, Pt3 cluster and Pt SA for fundamentally understanding structure (especially the sub-nano structure) effects on n-butane DDH reaction at the atomic level. The as-prepared fully exposed Pt3 cluster catalyst shows higher conversion (35.4%) and remarkable alkene selectivity (99.0%) for n-butane direct DDH reaction at 450 °C, compared to typical Pt NP and Pt SA catalysts supported on ND@G. Density functional theory calculation (DFT) reveal that the fully exposed Pt3 clusters possess favorable dehydrogenation activation barrier of n-butane and reasonable desorption barrier of butene in the DDH reaction.
An RFC4/Notch1 signaling feedback loop promotes NSCLC metastasis and stemness
Authors: Liu Lei , Tao Tianyu , Liu Shihua , Yang Xia , Chen Xuwei , Liang Jiaer , Hong Ruohui , Wang Wenting , Yang Yi , Li Xiaoyi , Zhang Youhong , Li Quanfeng , Liang Shujun , Yu Haocheng , Wu Yun , Guo Xinyu , Lai Yan , Ding Xiaofan , Guan Hongyu , Wu Jueheng , Zhu Xun , Yuan Jie , Li Jun , Su Shicheng , Li Mengfeng , Cai Xiuyu , Cai Junchao , Tian Han 
Published: May 2021

AbstractNotch signaling represents a key mechanism mediating cancer metastasis and stemness. To understand how Notch signaling is overactivated to couple tumor metastasis and self-renewal in NSCLC cells, we performed the current study and showed that RFC4, a DNA replication factor amplified in more than 40% of NSCLC tissues, directly binds to the Notch1 intracellular domain (NICD1) to competitively abrogate CDK8/FBXW7-mediated degradation of NICD1. Moreover, RFC4 is a functional transcriptional target gene of Notch1 signaling, forming a positive feedback loop between high RFC4 and NICD1 levels and sustained overactivation of Notch signaling, which not only leads to NSCLC tumorigenicity and metastasis but also confers NSCLC cell resistance to treatment with the clinically tested drug DAPT against NICD1 synthesis. Furthermore, together with our study, analysis of two public datasets involving more than 1500 NSCLC patients showed that RFC4 gene amplification, and high RFC4 and NICD1 levels were tightly correlated with NSCLC metastasis, progression and poor patient prognosis. Therefore, our study characterizes the pivotal roles of the positive feedback loop between RFC4 and NICD1 in coupling NSCLC metastasis and stemness properties and suggests its therapeutic and diagnostic/prognostic potential for NSCLC therapy.
YAP promotes the activation of NLRP3 inflammasome via blocking K27-linked polyubiquitination of NLRP3
Authors: Wang Dan , Zhang Yening , Xu Xueming , Wu Jianfeng , Peng Yue , Li Jing , Luo Ruiheng , Huang Lingmin , Liu Liping , Yu Songlin , Zhang Ningjie , Lu Ben , Zhao Kai 
Published: May 2021

AbstractThe transcription coactivator YAP plays a vital role in Hippo pathway for organ-size control and tissue homeostasis. Recent studies have demonstrated YAP is closely related to immune disorders and inflammatory diseases, but the underlying mechanisms remain less defined. Here, we find that YAP promotes the activation of NLRP3 inflammasome, an intracellular multi-protein complex that orchestrates host immune responses to infections or sterile injuries. YAP deficiency in myeloid cells significantly attenuates LPS-induced systemic inflammation and monosodium urate (MSU) crystals-induced peritonitis. Mechanistically, YAP physically interacts with NLRP3 and maintains the stability of NLRP3 through blocking the association between NLRP3 and the E3 ligase β-TrCP1, the latter increases the proteasomal degradation of NLRP3 via K27-linked ubiquitination at lys380. Together, these findings establish a role of YAP in the activation of NLRP3 inflammasome, and provide potential therapeutic target to treat the NLRP3 inflammasome-related diseases.
Metal-organic framework membranes with single-atomic centers for photocatalytic CO2 and O2 reduction
Authors: Hao Yu-Chen , Chen Li-Wei , Li Jiani , Guo Yu , Su Xin , Shu Miao , Zhang Qinghua , Gao Wen-Yan , Li Siwu , Yu Zi-Long , Gu Lin , Feng Xiao , Yin An-Xiang , Si Rui , Zhang Ya-Wen , Wang Bo , Yan Chun-Hua 
Published: May 2021

AbstractThe demand for sustainable energy has motivated the development of artificial photosynthesis. Yet the catalyst and reaction interface designs for directly fixing permanent gases (e.g. CO2, O2, N2) into liquid fuels are still challenged by slow mass transfer and sluggish catalytic kinetics at the gas-liquid-solid boundary. Here, we report that gas-permeable metal-organic framework (MOF) membranes can modify the electronic structures and catalytic properties of metal single-atoms (SAs) to promote the diffusion, activation, and reduction of gas molecules (e.g. CO2, O2) and produce liquid fuels under visible light and mild conditions. With Ir SAs as active centers, the defect-engineered MOF (e.g. activated NH2-UiO-66) particles can reduce CO2 to HCOOH with an apparent quantum efficiency (AQE) of 2.51% at 420 nm on the gas-liquid-solid reaction interface. With promoted gas diffusion at the porous gas-solid interfaces, the gas-permeable SA/MOF membranes can directly convert humid CO2 gas into HCOOH with a near-unity selectivity and a significantly increased AQE of 15.76% at 420 nm. A similar strategy can be applied to the photocatalytic O2-to-H2O2 conversions, suggesting the wide applicability of our catalyst and reaction interface designs.
The histone variant H2A.W and linker histone H1 co-regulate heterochromatin accessibility and DNA methylation
Authors: Bourguet Pierre , Picard Colette L. , Yelagandula Ramesh , Pélissier Thierry , Lorković Zdravko J. , Feng Suhua , Pouch-Pélissier Marie-Noëlle , Schmücker Anna , Jacobsen Steven E. , Berger Frédéric , Mathieu Olivier 
Published: May 2021

AbstractIn flowering plants, heterochromatin is demarcated by the histone variant H2A.W, elevated levels of the linker histone H1, and specific epigenetic modifications, such as high levels of DNA methylation at both CG and non-CG sites. How H2A.W regulates heterochromatin organization and interacts with other heterochromatic features is unclear. Here, we create a h2a.w null mutant via CRISPR-Cas9, h2a.w-2, to analyze the in vivo function of H2A.W. We find that H2A.W antagonizes deposition of H1 at heterochromatin and that non-CG methylation and accessibility are moderately decreased in h2a.w-2 heterochromatin. Compared to H1 loss alone, combined loss of H1 and H2A.W greatly increases accessibility and facilitates non-CG DNA methylation in heterochromatin, suggesting co-regulation of heterochromatic features by H2A.W and H1. Our results suggest that H2A.W helps maintain optimal heterochromatin accessibility and DNA methylation by promoting chromatin compaction together with H1, while also inhibiting excessive H1 incorporation.
On-chip spectrometers using stratified waveguide filters
Authors: Li Ang , Fainman Yeshaiahu 
Published: May 2021

AbstractWe present an ultra-compact single-shot spectrometer on silicon platform for sparse spectrum reconstruction. It consists of 32 stratified waveguide filters (SWFs) with diverse transmission spectra for sampling the unknown spectrum of the input signal and a specially designed ultra-compact structure for splitting the incident signal into those 32 filters with low power imbalance. Each SWF has a footprint less than 1 µm × 30 µm, while the 1 × 32 splitter and 32 filters in total occupy an area of about 35 µm × 260 µm, which to the best of our knowledge, is the smallest footprint spectrometer realized on silicon photonic platform. Experimental characteristics of the fabricated spectrometer demonstrate a broad operating bandwidth of 180 nm centered at 1550 nm and narrowband peaks with 0.45 nm Full-Width-Half-Maximum (FWHM) can be clearly resolved. This concept can also be implemented using other material platforms for operation in optical spectral bands of interest for various applications.
ZBP1 not RIPK1 mediates tumor necroptosis in breast cancer
Authors: Baik Jin Young , Liu Zhaoshan , Jiao Delong , Kwon Hyung-Joon , Yan Jiong , Kadigamuwa Chamila , Choe Moran , Lake Ross , Kruhlak Michael , Tandon Mayank , Cai Zhenyu , Choksi Swati , Liu Zheng-gang 
Published: May 2021

AbstractTumor necrosis happens commonly in advanced solid tumors. We reported that necroptosis plays a major role in tumor necrosis. Although several key necroptosis regulators including receptor interacting protein kinase 1 (RIPK1) have been identified, the regulation of tumor necroptosis during tumor development remains elusive. Here, we report that Z-DNA-binding protein 1 (ZBP1), not RIPK1, mediates tumor necroptosis during tumor development in preclinical cancer models. We found that ZBP1 expression is dramatically elevated in necrotic tumors. Importantly, ZBP1, not RIPK1, deletion blocks tumor necroptosis during tumor development and inhibits metastasis. We showed that glucose deprivation triggers ZBP1-depedent necroptosis in tumor cells. Glucose deprivation causes mitochondrial DNA (mtDNA) release to the cytoplasm and the binding of mtDNA to ZBP1 to activate MLKL in a BCL-2 family protein, NOXA-dependent manner. Therefore, our study reveals ZBP1 as the key regulator of tumor necroptosis and provides a potential drug target for controlling tumor metastasis.
Unified theory for light-induced halide segregation in mixed halide perovskites
Authors: Chen Zehua , Brocks Geert , Tao Shuxia , Bobbert Peter A. 
Published: May 2021

AbstractMixed halide perovskites that are thermodynamically stable in the dark demix under illumination. This is problematic for their application in solar cells. We present a unified thermodynamic theory for this light-induced halide segregation that is based on a free energy lowering of photocarriers funnelling to a nucleated phase with different halide composition and lower band gap than the parent phase. We apply the theory to a sequence of mixed iodine-bromine perovskites. The spinodals separating metastable and unstable regions in the composition-temperature phase diagrams only slightly change under illumination, while light-induced binodals separating stable and metastable regions appear signalling the nucleation of a low-band gap iodine-rich phase. We find that the threshold photocarrier density for halide segregation is governed by the band gap difference of the parent and iodine-rich phase. Partial replacement of organic cations by cesium reduces this difference and therefore has a stabilizing effect.
Gut microbiome modulates Drosophila aggression through octopamine signaling
Authors: Jia Yicong , Jin Shan , Hu Kunkun , Geng Lei , Han Caihong , Kang Ruxue , Pang Yuxin , Ling Erjun , Tan Eng King , Pan Yufeng , Liu Wei 
Published: May 2021

AbstractGut microbiome profoundly affects many aspects of host physiology and behaviors. Here we report that gut microbiome modulates aggressive behaviors in Drosophila. We found that germ-free males showed substantial decrease in inter-male aggression, which could be rescued by microbial re-colonization. These germ-free males are not as competitive as wild-type males for mating with females, although they displayed regular levels of locomotor and courtship behaviors. We further found that Drosophila microbiome interacted with diet during a critical developmental period for the proper expression of octopamine and manifestation of aggression in adult males. These findings provide insights into how gut microbiome modulates specific host behaviors through interaction with diet during development.
Unravelling the electron injection/transport mechanism in organic light-emitting diodes
Authors: Sasaki Tsubasa , Hasegawa Munehiro , Inagaki Kaito , Ito Hirokazu , Suzuki Kazuma , Oono Taku , Morii Katsuyuki , Shimizu Takahisa , Fukagawa Hirohiko 
Published: May 2021

AbstractAlthough significant progress has been made in the development of light-emitting materials for organic light-emitting diodes along with the elucidation of emission mechanisms, the electron injection/transport mechanism remains unclear, and the materials used for electron injection/transport have been basically unchanged for more than 20 years. Here, we unravelled the electron injection/transport mechanism by tuning the work function near the cathode to about 2.0 eV using a superbase. This extremely low-work function cathode allows direct electron injection into various materials, and it was found that organic materials can transport electrons independently of their molecular structure. On the basis of these findings, we have realised a simply structured blue organic light-emitting diode with an operational lifetime of more than 1,000,000 hours. Unravelling the electron injection/transport mechanism, as reported in this paper, not only greatly increases the choice of materials to be used for devices, but also allows simple device structures.
Community composition of microbial microcosms follows simple assembly rules at evolutionary timescales
Authors: Meroz Nittay , Tovi Nesli , Sorokin Yael , Friedman Jonathan 
Published: May 2021

AbstractManaging and engineering microbial communities relies on the ability to predict their composition. While progress has been made on predicting compositions on short, ecological timescales, there is still little work aimed at predicting compositions on evolutionary timescales. Therefore, it is still unknown for how long communities typically remain stable after reaching ecological equilibrium, and how repeatable and predictable are changes when they occur. Here, we address this knowledge gap by tracking the composition of 87 two- and three-species bacterial communities, with 3–18 replicates each, for ~400 generations. We find that community composition typically changed during evolution, but that the composition of replicate communities remained similar. Furthermore, these changes were predictable in a bottom-up approach—changes in the composition of trios were consistent with those that occurred in pairs during coevolution. Our results demonstrate that simple assembly rules can hold even on evolutionary timescales, suggesting it may be possible to forecast the evolution of microbial communities.
Mechanisms of halosulfuron methyl pesticide biosorption onto neem seeds powder
Authors: Haq Atta ul , Saeed Muhammad , Usman Muhammad , Zahoor Ameer Fawad , Anjum Muhammad Naveed , Maqbool Tahir , Naheed Shazia , Kashif Muhammad 
Published: May 2021

AbstractThe current investigation was designed to remove halosulfuron methyl from aqueous media by means of neem seed powder (NSP) in batch modes. Characterizations of NSP were carried out by using EDX, SEM, FTIR, point of zero charge and surface analysis. Optimum operation conditions were scrutinized by studying the influence of different factors like solution pH, dose of NSP, contact time, initial halosulfuron methyl concentration and temperature. Result indicates the dependency of the removal of halosulfuron methyl on solution pH and maximal removal (54%) was achieved in acidic medium (i.e. pH 3.0). To identify the chemical surface of NSP, point of zero charge of NSP was determined and was found to be 6.5 which imply that the surface of NSP is positively charged below pH 6.6 and favored the anionic sorption. Kinetics of halosulfuron methyl were demonstrated well by pseudo second order due to highest R2 (0.99) owing to the nearness between experimental and calculated sorption capacities. Isotherm results imply that Langmuir was found to the principal model to explain the removal of halosulfuron methyl and maximum monolayer sorption capacity was determined to be 200 mg g−1. Thermodynamic parameters like ΔH°, ΔG° and ΔS° were calculated from van’t Hoff plot and were found negative which suggest that removal of halosulfuron methyl is exothermic and spontaneous at low temperature. These outcomes insinuate that neem seed power may be a valuable, inexpensive and ecofriendly biosorbent for the removal of pesticides.
A unified resource and configurable model of the synapse proteome and its role in disease
Authors: Sorokina Oksana , Mclean Colin , Croning Mike D. R. , Heil Katharina F. , Wysocka Emilia , He Xin , Sterratt David , Grant Seth G. N. , Simpson T. Ian , Armstrong J. Douglas 
Published: May 2021

AbstractGenes encoding synaptic proteins are highly associated with neuronal disorders many of which show clinical co-morbidity. We integrated 58 published synaptic proteomic datasets that describe over 8000 proteins and combined them with direct protein–protein interactions and functional metadata to build a network resource that reveals the shared and unique protein components that underpin multiple disorders. All the data are provided in a flexible and accessible format to encourage custom use.
DNA methylation landscape of 16 canine somatic tissues by methylation-sensitive restriction enzyme-based next generation sequencing
Authors: Yamazaki Jumpei , Matsumoto Yuki , Jelinek Jaroslav , Ishizaki Teita , Maeda Shingo , Watanabe Kei , Ishihara Genki , Yamagishi Junya , Takiguchi Mitsuyoshi 
Published: May 2021

AbstractDNA methylation plays important functions in gene expression regulation that is involved in individual development and various diseases. DNA methylation has been well studied in human and model organisms, but only limited data exist in companion animals like dog. Using methylation-sensitive restriction enzyme-based next generation sequencing (Canine DREAM), we obtained canine DNA methylation maps of 16 somatic tissues from two dogs. In total, we evaluated 130,861 CpG sites. The majority of CpG sites were either highly methylated (> 70%, 52.5–64.6% of all CpG sites analyzed) or unmethylated (< 30%, 22.5–28.0% of all CpG sites analyzed) which are methylation patterns similar to other species. The overall methylation status of CpG sites across the 32 methylomes were remarkably similar. However, the tissue types were clearly defined by principle component analysis and hierarchical clustering analysis with DNA methylome. We found 6416 CpG sites located closely at promoter region of genes and inverse correlation between DNA methylation and gene expression of these genes. Our study provides basic dataset for DNA methylation profiles in dogs.
Associations among perfluorooctanesulfonic/perfluorooctanoic acid levels, nuclear receptor gene polymorphisms, and lipid levels in pregnant women in the Hokkaido study
Authors: Kobayashi Sumitaka , Sata Fumihiro , Goudarzi Houman , Araki Atsuko , Miyashita Chihiro , Sasaki Seiko , Okada Emiko , Iwasaki Yusuke , Nakajima Tamie , Kishi Reiko 
Published: May 2021

AbstractThe effect of interactions between perfluorooctanesulfonic (PFOS)/perfluorooctanoic acid (PFOA) levels and nuclear receptor genotypes on fatty acid (FA) levels, including those of triglycerides, is not clear understood. Therefore, in the present study, we aimed to analyse the association of PFOS/PFOA levels and single-nucleotide polymorphisms (SNPs) in nuclear receptors with FA levels in pregnant women. We analysed 504 mothers in a birth cohort between 2002 and 2005 in Japan. Serum PFOS/PFOA and FA levels were measured using liquid chromatography-tandem mass spectrometry and gas chromatography-mass spectrometry. Maternal genotypes in PPARA (rs1800234; rs135561), PPARG (rs3856806), PPARGC1A (rs2970847; rs8192678), PPARD (rs1053049; rs2267668), CAR (rs2307424; rs2501873), LXRA (rs2279238) and LXRB (rs1405655; rs2303044; rs4802703) were analysed. When gene-environment interaction was considered, PFOS exposure (log10 scale) decreased palmitic, palmitoleic, and oleic acid levels (log10 scale), with the observed β in the range of − 0.452 to − 0.244; PPARGC1A (rs8192678) and PPARD (rs1053049; rs2267668) genotypes decreased triglyceride, palmitic, palmitoleic, and oleic acid levels, with the observed β in the range of − 0.266 to − 0.176. Interactions between PFOS exposure and SNPs were significant for palmitic acid (Pint = 0.004 to 0.017). In conclusion, the interactions between maternal PFOS levels and PPARGC1A or PPARD may modify maternal FA levels.
Numerical simulation of spatiotemporal red blood cell aggregation under sinusoidal pulsatile flow
Authors: Lee Cheong-Ah , Paeng Dong-Guk 
Published: May 2021

AbstractPrevious studies on red blood cell (RBC) aggregation have elucidated the inverse relationship between shear rate and RBC aggregation under Poiseuille flow. However, the local parabolic rouleaux pattern in the arterial flow observed in ultrasonic imaging cannot be explained by shear rate alone. A quantitative approach is required to analyze the spatiotemporal variation in arterial pulsatile flow and the resulting RBC aggregation. In this work, a 2D RBC model was used to simulate RBC motion driven by interactional and hydrodynamic forces based on the depletion theory of the RBC mechanism. We focused on the interaction between the spatial distribution of shear rate and the dynamic motion of RBC aggregation under sinusoidal pulsatile flow. We introduced two components of shear rate, namely, the radial and axial shear rates, to understand the effect of sinusoidal pulsatile flow on RBC aggregation. The simulation results demonstrated that specific ranges of the axial shear rate and its ratio with radial shear rate strongly affected local RBC aggregation and parabolic rouleaux formation. These findings are important, as they indicate that the spatiotemporal variation in shear rate has a crucial role in the aggregate formation and local parabolic rouleaux under pulsatile flow.
A study on the effects of regional differences on agricultural water resource utilization efficiency using super-efficiency SBM model
Authors: Huang Yujie , Huang Xianke , Xie Munan , Cheng Wei , Shu Qin 
Published: May 2021

AbstractThis study evaluated the water resource utilization efficiency and resource consumption for planting, forestry, animal husbandry, and fishery in various regions of China. Using the super-efficiency Slacks-Based Measure (SBM) analysis method, the main agricultural pollution emissions (Chemical Oxygen Demand, ammonia nitrogen, and agricultural carbon emissions) were proposed as environmental constraints for the first time. The threshold regression model was used to measure the impact of agricultural water use efficiency on agricultural water consumption by constructing seven different explanatory variables. The results show that the overall utilization efficiency of agricultural water resources in China presents a fluctuating downward trend, and the regional differences are significant. A single threshold effect on agricultural water consumption was found in five variables: per capita water resources, disposable income, dependence on foreign trade, industrial structure, and Gross Domestic Product. The increase in each parameter will have a positive effect on agricultural water consumption. The relationship between agricultural water use efficiency and agricultural water use was non-linear when the government's attention and the rural labor force were used as threshold variables.