bluuebunny/crossref_metadata_2025_split huggingface dataset:
Perinatal outcomes following immediate delivery or expectant management of preterm premature rupture of membranes during the late preterm period
Authors: Kwon Hayan , Kang Suk Ho , Ko Hyun Sun , Kwon Ja Young , Kwon Han-sung , Oh Kyung Joon , Oh Minkyung , Oh Soo-young , Lee Mi-Young , Choi Soo Ran , Han You Jung , Sul Ah-Ram , Jung Youjin , Kim Jimin , Park Ji Jeong , Lee Jayoun , Kim Young Nam , Yang Jeong In 
Published: February 2021

Abstract
The aim of this study is to evaluate maternal and neonatal outcomes following immediate delivery or expectant management of preterm premature rupture of membranes (PPROM) during the late preterm period at 34+ 0–36+ 6 weeks of pregnancy. We conducted a retrospective study on singleton pregnancies with PPROM during the late preterm period using medical records at twelve tertiary medical centres in Korea from January 2007 to December 2016. Data on demographic characteristics and outcome measures were collected. The primary outcomes were maternal sepsis for maternal outcome and neonatal sepsis and neonatal death for neonatal outcomes. Of the 1,072 women, 782 cases (72.9%) were assigned to the immediate delivery group, and 290 cases (27.1%) were categorized into the expectant management group. There was a significant difference in the rate of clinical neonatal sepsis (immediate delivery, 3.8% vs expectant management, 15.8%; p < 0.0001), however, no differences in maternal sepsis (p = 0.5424), culture-proven neonatal sepsis (p = 0.2108), or neonatal death (p = 0.3899) were observed. In conclusion, expectant management in women with PPROM during the late preterm period does not increase the risk of severe maternal and neonatal morbidities and mortality; however, careful monitoring for chorioamnionitis or fetal compromise should be considered during expectant management.
Assessment of Liver Fibrosis by Transient Elastography in Children with Chronic Hepatitis B Virus Infection
Authors: Xu Zhiqiang , Zhao Jinfang , Liu Jiaye , Dong Yi , Wang Fuchuan , Yan Jianguo , Cao Lili , Wang Pu , Li Aiqin , Li Jing , Zhu Shishu , Zhong Yanwei , Zhang Min , Wang Fu-sheng 
Published: February 2021

Abstract
Background: This study aimed to investigate the effectiveness of transient elastography (TE) by comparing liver biopsies to assess liver fibrosis in children with chronic hepatitis B (CHB). Methods: A total of 157 CHB children aged 0 - 6 years in China were enrolled in this single-center prospective study. All patients underwent liver stiffness measurement (LSM) by TE and liver biopsy at an interval of less than a week. Results: LSM, aspartate aminotransferase (AST)-platelet ratio index (APRI), and fibrosis-4 score (FIB-4) positively correlated with activity grade and fibrosis stage in children with CHB. The area under receiver operating characteristic curves (AUCs) of LSM for identifying significant (F ≥ 2) and advanced fibrosis (F ≥ 3) were 0.732 and 0.94, the cut-off values were 5.6 kPa and 6.9 kPa, specificity of 75.7% and 91.5%, and sensitivity of 67.4% and 81.3%, respectively. Compared to LSM, the overall diagnostic performance of APRI and FIB-4 for significant and advanced fibrosis was suboptimal with low AUCs and sensitivity. Since LSM, platelet, and Log10HBsAg were independent factors with the fibrosis stages (F < 2 and F ≥ 2) on the liver biopsy, the LPS index was formulated to predict F ≥ 2 by combining LSM, platelet, and Log10HBsAg. The AUC of LPS for F ≥ 2 was increased to 0.792, which was higher than that of LSM (0.732, p < 0.05), with an improved sensitivity (76.6% vs 67.4%).Conclusions: TE represents a promising technology for the diagnosis of advanced fibrosis in CHB children aged 0 - 6 years.
Prevalence of hypogammaglobulinemia and its management with subcutaneous immunoglobulin supplementation in patients after allogeneic hematopoietic stem cell transplantation- a single-center analysis
Authors: Karakulska-Prystupiuk Ewa Marta , Dwilewicz-Trojaczek Jadwiga , Drozd-Sokołowska Joanna , Kmin Ewelina , Chlebus Marcin , Szczypińska Karolina , Boguradzki Piotr , Tomaszewska Agnieszka , Mądry Krzysztof , Bilinski Jarosław , Basak Grzegorz Władysław , Jędrzejczak Wiesław Wiktor 
Published: February 2021

Abstract
PURPOSE Secondary immunodeficiencies are frequently observed after allo-HSCT. This study aimed to assess the prevalence and management of hypogammaglobulinemia requiring immunoglobulin.METHODS A retrospective single-institution study involved 126 adult patients transplanted in 2012–2019 for hematological malignancies. Patients were tested every 2–3 weeks for plasma IgG concentration during the 1st year after transplantation and supplemented with facilitated subcutaneous immunoglobulin when they met pre-defined criteria.RESULTS The IgG concentration < 500mg/dL was diagnosed in 41 patients, while 500700mg/dL in 25 and altogether 53 patients required IgG supplementation. The median number of IgG administrations was 2. The median time to the first IgG administration after allo-HSCT was 4.1 months, while to the next administration (if more than one was required)- 53 days (prophylactic group) and 32 days(group with infections). We did not observe any significant toxicity. The 1-y overall survival in the supplemented group with infections and IgG 500-700mg/dL was significantly lower than compared to no-need immunoglobulin group (p = 0.009). The diagnosis with Acute Lymphoblastic Leukemia (ALL) or Chronic Lymphocytic Leukemia (CLL) and the use of systemic corticosteroids were associated with the average probability of hypogammaglobulinemia requiring IgG supplementation in 83.8% (versus 39.3%, p = 0.000 for other diagnosis) and 64.2% (versus 31.5%, p = 0.005 for patients without systemic corticosteroids) respectively.CONCLUSION Over 40% of the adult recipients may require at least incidental immunoglobulin supplementation during the first year after allo-HSCT. Low IgG concentrations are associated with inferior outcomes. The subcutaneous route of IgG administration appeared to be safe and may allow for long persistence.
Stereotactic Body Radiation Therapy as a Salvage Treatment for Single Viable Hepatocellular Carcinoma at the Site of Incomplete Transarterial Chemoembolization
Authors: Lee Sumin , Jung Jinhong , Park Jin-hong , Kim So Yeon , Choi Jonggi , Lee Danbi , Shim Ju Hyun , Kim Kang Mo , Lim Young-Suk , Lee Han Chu , Park Hee Hyun , Kim Jong Hoon , Yoon Sang Min 
Published: February 2021

Abstract
Background: To evaluate the clinical outcomes of patients who received stereotactic body radiation therapy (SBRT) for single viable hepatocellular carcinoma (HCC) at the site of incomplete transarterial chemoembolization (TACE).Methods: Incomplete TACE was defined as (1) evidence of viable HCC at the site of TACE on follow-up images following one or more consecutive TACEs, (2) no definite tumor staining on celiac angiogram, or (3) no definite iodized oil uptake on post-embolization angiogram or computed tomography. A total of 302 patients were treated between 2012 and 2017 at Asan Medical Center (Seoul, South Korea). Doses of 10–15 Gy per fraction were given over 3–4 consecutive days. Treatment-related adverse events were evaluated according to the common terminology criteria for adverse events, version 4.03.Results: The median follow-up duration was 32.9 months (interquartile range [IQR], 23.6–41.7) and the median tumor size was 2.0 cm (range, 0.7–6.9). The local control (LC) and overall survival rates at 3 years were 91.2% and 72.7%, respectively. 95.4% of the tumors reached complete response (CR) during the entire follow-up period (anyCR). The median interval from SBRT to anyCR was 3.4 months (IQR, 1.9–4.7), and 39.9% and 83.3% of the lesions reached CR at 3- and 6-months after SBRT, respectively. Radiation-induced liver disease was observed in 8 (2.6%) patients. No patients experienced gastroduodenal bleeding within the radiation field.Conclusion: SBRT should be considered a feasible salvage treatment option for HCC after incomplete TACE.
Laser-assisted Preparation of C3N4/Fe2O3/Au&nbsp;Nanocomposite: A Magnetic Reusable Catalyst for Pollutant Degradation
Authors: Nasri Atefeh , Nezafat Zahra , Jaleh Babak , Orooji Yasin , Varma Rajender S. 
Published: February 2021

Abstract
Assorted common contaminants namely organic dyes and nitro compounds are generated by various industries and have caused alarming problems for the environment and humans. The development of effective and newer methods to eliminate these contaminants is imperative. One effective way to eliminate these pollutants is to deploy sustainable heterogeneous catalyst systems. Accordingly, a novel and efficient magnetic nanocomposite catalyst comprising graphitic carbon nitride (g-C3N4), gold (Au), and hematite (α-Fe2O3) nanoparticles (C3N4/Fe2O3/Au) is prepared through a cost-effective and green method. The individual components, g-C3N4 and Au nanoparticles (NPs), are prepared via thermal decomposition of urea and laser ablation in liquid (LAL), respectively. The behavior of C3N4/Fe2O3/Au nanocomposite as a catalyst for the borohydride reduction of methyl orange (MO) dye and 4-nitrophenol (4-NP) in aqueous solution is illustrated.
Real-World Clinical Performance of the Abbott Panbio with Nasopharyngeal, Throat and Saliva Swabs Among Symptomatic Individuals with COVID-19
Authors: Stokes William , Berenger Byron M. , Portnoy Danielle , Scott Brittney , Szelewicki Jonas , Singh Takshveer , Venner Allison A. , Turnbull LeeAnn , Pabbaraju Kanti , Shokoples Sandy , Wong Anita A. , Gill Kara , Guttridge Tracy , Proctor Dustin , Hu Jia , Tipples Graham 
Published: February 2021

Abstract
BACKGROUND
Point of Care SARS-CoV-2 antigen tests, such as the Abbott Panbio, have great potential to help combat the COVID-19 pandemic. The Panbio is Health Canada approved for the detection of SARS-CoV-2 in symptomatic individuals within the first 7 days of COVID-19 symptom onset(s).
METHODS
Symptomatic adults recently diagnosed with COVID-19 in the community were recruited into the study. Paired nasopharyngeal (NP), throat, and saliva swabs were collected, with one paired swab tested immediately with the Panbio, and the other transported in universal transport media and tested using reverse-transcriptase polymerase chain reaction (RT-PCR). We also prospectively evaluated results from assessment centres. For those individuals, an NP swab was collected for Panbio testing and paired with RT-PCR results from parallel NP or throat swabs.
RESULTS
145 individuals were included in the study. Collection of throat and saliva was stopped early due to poorer performance (throat sensitivity 57.7%, n = 61, and saliva sensitivity 2.6%, n = 41). NP swab sensitivity was 87.7% [n = 145, 95% confidence interval (CI) 81.0% − 92.7%]. There were 1,641 symptomatic individuals tested by Panbio in assessment centres, with 268/1641 (16.3%) positive for SARS-CoV-2. There were 37 false negatives and 2 false positives, corresponding to a sensitivity and specificity of 86.1% [95% CI 81.3% − 90.0%] and 99.9% [95% CI 99.5% − 100.0%], respectively.
CONCLUSIONS
The Panbio test reliably detects most cases of SARS-CoV-2 from adults in the community setting presenting within 7 days of symptom onset using nasopharyngeal swabs. Throat and saliva swabs are not reliable specimens for the Panbio.
Fto in the hippocampus mediates depression-like behaviors
Authors: Liu Shu , Xiu Jianbo , Zhu Caiyun , Li Chen , Meng Kexin , Du Tingfu , Han Rongrong , Li Lanlan , Xu Lingdan , Shen Yan , Xu Qi 
Published: February 2021

Abstract
Dynamic and reversible RNA methylation has emerged as a new layer of epigenetic regulation of behaviors such as learning and memory; however, whether RNA methylation plays a critical role in the pathophysiology of depression is unclear. Here, we report that expression of the fat mass and obesity associated gene (FTO), a primary RNA demethylase, is downregulated in the hippocampi of both major depressive disorder (MDD) patients and mouse models of depression. Suppressing Fto expression in the hippocampus induces depression-like behaviors in mice, while elevating its expression leads to antidepressant effects. Epitranscriptomic profiling of N6-methyladenosine (m6A) RNA methylation in the hippocampi of Fto knockdown (KD), Fto knockout (cKO), and Fto-overexpressing (OE) mice identified adrenoceptor beta 2 (Adrb2) mRNA as a target of Fto. Adrb2 stimulation reverses the depression-like behaviors and spine loss induced by hippocampal Fto deficiency, possibly via the modulation of hippocampal Sirt1 expression by c-Myc. These findings reveal that Fto in the hippocampus mediates depression-like behaviors and highlight the importance of reversible RNA methylation in driving depression.
Study on The Effect of Different Type of Sugar on Lipid Deposition in Goose Fatty Liver
Authors: Wei Rongxue , Deng Donghang , Teng Yongqiang , Lu Cangcang , Luo Zhaoyun , Abdulai Mariama , Liu Hehe , Xu Hongyong , Li Liang , Hu Shengqiang , Hu Jiwei , Wei Shouhai , Han Chunchun 
Published: February 2021

Abstract
Backgroud: Early research in our lab is indicated that the effect of glucose, fructose and sucrose on the levels of triacylglycerol and inflammatory factor was significantly different, and it is speculated that the regulatory mechanism of lipid deposition by different type of sugar in the liver is different. Methods: In order to explore lipid deposition difference mediated by different type of sugar (glucos, fructose and sucrose) in goose fatty liver formation, this experiment was performed from cell culture, overfeeding experiment and transcriptome analysis three level. Results: Cell culture experiment results indicated that the levels of intracellular triglyceride (TG), total cholesterol (T-CHO) and lipid content of fructose treatment and sucrose treatment were significantly higher than those of glucose treatment (P < 0.05). In slaughter performance, the liver weight, the ratio of liver weight to body weight, feed conversion ratio (liver weight / feed consumption ) were better in sucrose overfeeding group (P < 0.05). In addition, the liver of the sucrose overfeeding group contained a lot of unsaturated fatty acids, especially (n-3) polyunsaturated fatty acids (n-3 PUFA ) (P < 0.05). Transcriptome analysis shown that the PPAR signaling pathway is highly enriched in the fructose and sucrose overfeeding groups; cell cycle and DNA replication pathways were highly enriched in the glucose overfeeding group. Conclusions: Due to lipids outward transportation decrease and anti-imflammation of unsaturated fatty acids (UFA), thereby, fructose and sucrose hve better ability to induce steatosis in foie gras formation.
Lower serum bilirubin is associated with poor renal outcome in IgA nephropathy patients: A retrospective study
Authors: Jiang Zheng , Tan Jiaxing , Wang Siqing , Dong Lingqiu , Han Xin , Tang Yi , Qin Wei 
Published: February 2021

Abstract
Background: IgA nephropathy (IgAN) is the most prevalent primary glomerulonephritis worldwide. It is meaningful to identify risk factors related to the development of IgAN. We conducted this study to explore the relationship between serum bilirubin and renal outcome of patients with IgAN.Methods: In this retrospective observational study, 1492 biopsy proven IgAN patients were recruited and divided into two groups according to their median baseline serum bilirubin concentration: the low bilirubin group (serum bilirubin≤9.7umol/L, n=753) and high bilirubin group (serum bilirubin>9.7umol/L, n=739). Basic clinical characteristics were assessed at the time of renal biopsy and the relationships between serum bilirubin and the combined endpoints were analyzed. In addition, propensity score matching (PSM) was performed to improve balance and simulate randomization between patients in different groups. Kaplan-Meier survival analysis was applied to explore the role of serum bilirubin in the progression of IgAN. Three models (including demographic, clinical, pathological features and serum bilirubin) of multivariate Cox regression analysis were established to evaluate the association of serum bilirubin and renal prognosis of IgAN.Results: During median 5-year follow-up period, significant differences were shown in Kaplan-Meier analysis. In the unmatched group, 189 (12.7%) patients progressed to the renal combined endpoints. Among this, 122 in 753 patients (16.2%) were in low bilirubin group and 67 in 739 patients (9.1%) were in high bilirubin group (p<0.001). After PSM, there were 134 (11.8%) patients reached the combined endpoints, which included 77 in 566 patients (14.6%) in low bilirubin group and 57 in 566 patients (10.1%) in high bilirubin group (p=0.039). The results of three models (including demographics, pathological, clinical indicators and serum bilirubin) demonstrated that a lower basic serum bilirubin level was significantly associated with a higher risk of reaching combined endpoints in IgAN patients both in unmatched and matched cohort. Conclusion: Serum bilirubin level may be negatively associated with the progression of IgAN.
Carriage of Cdt-B Encoding Campylobacter spp., Salmonellaenterica, and Yersinia entercolitica in Patients with Gastroenteritis, Irritable Bowel Syndrome, and Controls
Authors: Ganji Leila , Shirazi Mohmmad Hassan , Alebouyeh Masoud , Eslami Parisa , Daryani Naser Ebrahim , Rahbar Mohammad , Zali Mohammad Reza 
Published: February 2021

Abstract
Background: Cytolethal distending toxin (Cdt) is one of the bacterial toxins that present in a variety of Gram-negative human pathogens, such as E. coli, Salmonella spp., and Campylobacter spp. CDT composed of three subunits encoded by three adjacent genes, including cdtA, cdtB and cdtC. It is approved that cdtB had toxic activity and caused DNA damage of the host cell. Despite its presence in different bacterial species, role of Cdt in acute and chronic infections, such as gastroenteritis and irritable bowel syndrome (IBS), is unclear. To analyze this correlation, we studied the prevalence of cdtB among different enteropathogenic bacteria in patients with gastroenteritis and IBS compared with healthy people. Materials and Methods: In this cross-sectional descriptive study, 230 stool samples were collected from patients with gastroenteritis, IBS, and healthy people. The presence of Cdt-B encoding bacteria, including Escherichia coli, Campylobacter spp., Yersinia entercolitica, Providencia alkalifacience, and Salmonella enterica was examined by polymerase chain reaction. Demographic data and type of disease was collected through interview and a questionnaire.Results: Out of 230 stool samples, Cdt-B encoding Campylobacter spp. were found in 34.6% (52/150), 6.25% (5/80), and 4% (2/50) of the patients with gastroenteritis, IBS, and the control group, respectively. Carriage of Cdt-B encoding Salmonella enterica was characterized among 5.3% (8/150) of the patients with gastroenteritis and 17.5% (14/80) of the IBS patients. Although none of the patients carried cdtB of E. coli and Providencia spp., cdtB of Y. enterocolitica was detected in 1 of the patients with gastroenteritis (0.6%). Statistical analysis showed significant correlation between infection with CdtB-encoding Campylobacter spp. and IBS-D subtype. No significant correlation was found between infection with Cdt-B encoding bacteria, and other clinical and demographic data.Conclusions: Our results confirmed relatively higher frequency of Cdt-B encoding bacteria in the intestine of IBS patients and those with gastroenteritis compared with healthy individuals. Regarding the frequency of Cdt-B encoding Salmonella and Campylobacter bacteria, it was proposed that infection with these enteropathogens could be considered as a risk factor for the development or progression of IBS among the Iranian patients. Further studies are needed to establish this involvement.
Does Conservative Kidney Management Offer a Quantity or Quality of Life Benefit Compared to Dialysis? A Systematic Review
Authors: Engelbrecht Buur Louise , Kristian Madsen Jens , Inge Eidemak , Elizabeth Krarup , Guldager Lauridsen Thomas , Helbo Taasti Lena , Jeanette Finderup 
Published: February 2021

Abstract
Background Patients with stage 5 chronic kidney disease (CKD5) collaborate with their clinicians when choosing their future treatment modality. Most elderly patients with CKD5 may only have two treatment options: dialysis or conservative kidney management (CKM). The objective of this systematic review was to investigate whether CKM offers a quantity or quality of life benefit compared to dialysis for some patients with CKD5.MethodsThe databases MEDLINE, EMBASE, the Cochrane Library, and CINAHL were systematically searched for studies comparing patients with CKD5 treated with CKM or dialysis. The primary outcomes were mortality and quality of life (QoL). Hospitalization, symptom burden, and place of death were secondary outcomes. For studies reporting hazard ratios, pooled values were calculated, and forest plots conducted.ResultsTwenty-four primary studies, all observational, were identified. All studies except one reported an increased mortality in patients treated with CKM (pooled hazard ratio 0.53, 95% confidence interval 0.41-0.69). For patients aged ≥ 80 years and for elderly individuals with comorbidities, results were ambiguous. In most studies, CKM seemed advantageous for QoL and secondary outcomes. Findings were limited by the heterogeneity of studies and biased outcomes favouring dialysis.ConclusionsIn general, patients with CKD5 live for a shorter time on CKM than on dialysis. In patients aged ≥ 80 years old, and in elderly individuals with comorbidities, the survival benefits of dialysis seem to be lost. Regarding QoL, symptom burden, hospitalization, and place of death, CKM may have advantages. Higher quality studies are needed to guide patients and clinicians in the decision-making process.
Prognostic Stratification for IDH-wild-type Lower-grade Astrocytoma by Sanger Sequencing and Copy-number Variation Analysis with MLPA.
Authors: Makino Yasuhide , Arakawa Yoshiki , Yoshioka Ema , Shofuda Tomoko , Kawauchi Takeshi , Terada Yukinori , Tanji Masahiro , Kanematsu Daisuke , Mineharu Yohei , Miyamoto Susumu , Kanemura Yonehiro 
Published: February 2021

Abstract
The characteristics of IDH-wild-type lower-grade astrocytoma remain unclear. According to cIMPACT-NOW update 3, IDH-wild-type astrocytomas with any of the following factors show poor prognosis: combination of chromosome 7 gain and 10 loss (+ 7/-10), and/or EGFR amplification, and/or TERT promoter (TERTp) mutation. Multiplex ligation-dependent probe amplification (MLPA) can detect copy number variations with a reasonable cost. The purpose of this study was to find precise and cost-effective method for stratifying the prognosis of IDH-wild-type astrocytomas. Sanger sequencing, MLPA, and quantitative methylation-specific PCR were performed for 42 IDH-wild-type lower-grade astrocytomas surgically treated at Kyoto University Hospital, and overall survival was analyzed for 40 patients who underwent first surgery. Of the 42 IDH-wild-type astrocytomas, 21 were classified as grade 4 in cIMPACT-NOW update 3 criteria and all of them had either TERTp mutation or EGFR amplification. Kaplan-Meier analysis confirmed the prognostic significance of cIMPACT-NOW criteria, and WHO grade was also prognostic. Cox regression hazard model identified PTEN loss and PDGFRA amplification as independent significant prognostic indicators (Risk ratio of 9.75, p < 0.001 and 13.9, p = 0.002). The classification recommended by cIMPACT-NOW update 3 could be completed using Sanger sequencing and MLPA. Survival analysis revealed PTEN and PDGFRA were significant prognostic factors for IDH-wild-type lower-grade astrocytoma.