bluuebunny/crossref_metadata_2025_split huggingface dataset:
Oral Health Status of Patients Infected with Human Immunodeficiency Virus and Related Factors, Iran: A Cross- Sectional Study
Authors: Shaghaghian Soheila , Homayooni Mojtaba , Amin Maryam , Rezazadeh Fahimeh 
Published: July 2021

Abstract
Background: Human immunodeficiency virus (HIV) continues to be a major global issue. HIV-infected patients commonly experience oral health problems. This study aimed to assess oral health status of the patients and its related factors in Shiraz, Iran.Methods: In this cross-sectional study, by using simple random sampling, 250 HIV-infected patients were selected from Shiraz Voluntary Counseling and Testing Center in 2019. Patients' decayed, missing, and filled tooth (DMFT) index, plaque index (PI), and community periodontal index (CPI) were determined. Associations between patients' characteristics and the above-mentioned indices were examined by using Pearson correlation, one way ANOVA, chi-square, and independent sample T test. Results: Of the 222 participants, 111 (50%) had 15 or more missed teeth and 79 (35.6%) were edentulous. Their mean (SD) DMFT and PI were 20.71 (10.74) and 1.11 (0.79), respectively. About 40% of them had healthy gingiva. DMFT (p<0.001), PI (p<0.001), and CPI (p=0.002) were significantly worse in men than women. The patients' DMFT and edentulous status were significantly associated with their age (both p<0.001) and duration of disease (p=0.001 and p=0.008, respectively). Unemployed patients had the worst DMFT, PI, and CPI (all p<0.001) and the highest percentage of edentulous individuals (p=0.003). All examined indices were significantly worse in cigarette smokers, alcoholics, addicts, and patients with a previous history of imprisonment (all p<0.001).Conclusions: Overall, oral health status of the HIV-infected patients was unsatisfactory, which might indicate their inadequate access to dental services. Therefore, effective interventional programs are needed for prevention and early treatment of dental problems among this population, especially for more vulnerable groups such as older men with low socioeconomic status, and those with high-risk behaviors.
Chasing Hippos: Implications of YAP1 and TAZ Expression in Pregnancy-Associated Breast Cancer Tumorigenesis
Authors: Kyriazoglou Anastasios , Korakiti Anna-Maria , Papatheodoridi Alkistis-Maria , Apostolidou Kleoniki , Nonni Afroditi , Bletsa Garyfalia , Tsakogiannis Dimitris , Dimopoulos Meletios-Athanasios , Zagouri Flora 
Published: July 2021

Abstract
Background: Hippo signaling pathway de-regulation has been strongly associated with tumorigenesis, metastasis, and drug resistance. Pregnancy-associated breast cancer, the most frequently diagnosed malignancy during gestation, demonstrates an intricate molecular nature. The aim of the present study is to evaluate Hippo pathway transducers TAZ and YAP1 expression in pregnancy-associated breast cancer in relation to the clinicopathological characteristics of the disease.Methods: Formalin-fixed paraffin-embedded tissues from 21 PABC patients treated at Alexandra Hospital in Athens, Greece were immunohistochemically analyzed. Statistical analysis was performed to investigate a possible correlation among the Hippo pathway signaling and the clinical features of the disease.Results: In 48% of patients included in the study strong nuclear TAZ/YAP1 staining in tumor cells was identified. Additionally, the hormone receptor negative status was statistically correlated with strong positivity of the TAZ/YAP1 co-transcriptional factors. No association was observed with the overall-survival and the disease-free survival rate. Conclusions: Hippo pathway is proven to be de-regulated in a subset of pregnancy-associated breast cancer patients highlighting the complex molecular background of the disease which certainly requires further investigation.
Factor Analysis of Machining Parameters On Surface Integrity of CFRP Composites Based On A Microscopic Finite Element Cutting Model
Authors: Xiao Jianzhang , Wang Guifeng , Su Hang , Huang Pengcheng , Chen Zhongzhe 
Published: July 2021

Abstract
In the paper, a three-dimensional (3D) micromechanical finite element (FE) cutting model with three phases was developed to study the surface integrity of CFRP composites. The surface roughness and the depth of subsurface damage were predicted by using the FE cutting model, which were used to characterize the surface integrity. The machined surface observations and surface roughness measurements of CFRP composites at different fiber orientations were also performed for model validation. It is indicated that the 3D micromechanical model is capable of precisely predicting the surface integrity of CFRP composites. To investigate the complex coupling influences of multiple machining parameters on the surface integrity, the factor analysis of multiple machining parameters was performed, and then the effects of these machining parameters on the surface roughness and subsurface damage depth were obtained quantitatively. It was found that the fiber orientation angle and cutting speed are the most significant factors affecting the surface roughness, and the fiber orientation and edge radius are the main factors affecting the subsurface damage depth. The results also reveal that coupling effects of depth of cut and edge radius should be considered for improving the surface integrity of CFRP composites.
Synthesis of Bis (Benzoyl Acetone Ethylene Diimine) Schiff Base Complex of Nickel (II) Supported on Magnetite Silica Nano-Particles (Fe3O4@Sio2/Schiff-Base Of Ni (II)) and Using it as an Efficient Catalyst for Green Synthesis of 1-Amidoalkyl-2-Naphthol.
Authors: Setoodehkhah Moslem , Mazraati Amin , Moradian Mohsen 
Published: July 2021

Abstract
In this research, magnetite nano-particles as the core are fabricated by co-precipitation method from Fe(II) and Fe(III) chloride salts and then the surfaces of the nano-particles are modified to improve their performance. These modifications include the coating of silica and then the second layer by 3-amino propyl triethoxy silane (APTES) which causes the functionalization of magnetite nanoparticles with –NH2 groups. In the next step, an aliphatic tetra-dentate N2O2 Schiff base was synthesized by condensation reaction between benzoyl acetone and ethylenediamine. Then this Schiff base is immobilized on the magnetite silica surfaces. This immobilized Schiff base was converted to the Ni (II) Schiff base complex by the reaction with Ni(II) acetate tetrahydrate. This Ni (II) Schiff base complex is immobilized on magnetite silica and shown as Fe3O4@SiO2/Schiff base of Ni (II). This cor-shell nano-structure is fully characterized by techniques such as FT-IR, VSM, XRD, FE-SEM, EDX, TGA-DTA, AAS, BET, and BJH. In the last step, this nano-catalyst is used as an efficient catalyst for the solvent-free synthesis of amido alkyl naphthols by the condensation reaction between an aldehyde, 2-naphthol, and an amide. The reaction was monitored by TLC. At the end of the reaction, the nano-catalyst was removed easily from the reaction mixture by an external magnet. The products of amido alkyl naphthols were identified by FT-IR and 1HNMR techniques. Finally, the percentage yield of the reaction is calculated by measuring the mass of 1-amidoalkyl-2-naphthol synthesized in the presence of the catalyst.
Bifunctional Europium Doped SrTiO3 Ceramics with Energy Storage and Photoluminescence
Authors: Zhong Bingjie , Zuo Chengyang , Yang Chao , Yang Shilin , Li Yun , Yu Hongtao , Wei Xianhua 
Published: July 2021

Abstract
Rare-earth ion Eu 3+ was doped into SrTiO 3 ceramic to improve its dielectric energy storage properties and act as luminescence centres. Typically, SrTiO 3 ceramics doped with 0.2% Eu 3+ exhibit high breakdown strength up to 354 kV/cm and a relatively high recoverable energy density of 2.13 J/cm 3 . Compared to those of the undoped sample, the doped ceramic breakdown strength and recoverable energy density are enhanced by about 22% and 58%, respectively. The doping effect can be explained by the inhibition of the long-range movement of carriers. Moreover, the europium doped SrTiO 3 ceramic capacitor exhibits an excellent power density of 37 MW/cm 3 in an ultrafast discharge time of 25 nanoseconds at 200 kV/cm. Meanwhile, the Eu 3+ doped sample exhibits obvious red photoluminescence. The bifunctional ceramics offer an excellent prospect for energy storage and optical applications.
CircRNA-DOPEY2 Enhances The Chemosensitivity of Esophageal Cancer Cells By Inhibiting CPEB4-Mediated Mcl-1 Translation
Authors: Liu Zhenchuan , Gu Shaorui , Wu Kaiqin , Li Lei , Dong Chenglai , Wang Wenli , zhou yongxin 
Published: July 2021

Abstract
Background: Cisplatin-based chemotherapy is a mainstay systematic therapy for advanced esophageal squamous cell carcinoma (ESCC), and cisplatin resistance is not uncommon and the major barrier to patient outcome improvements. circRNAs are novel noncoding RNAs that are implicated in cancer progression, but their involvement in modulating cisplatin responsiveness in ESCC remains unknown. Methods: Bioinformatics analysis was used to profile and identify the circRNAs involved in cisplatin responsiveness in ESCC. The chemo-sensitive role of cDOPEY2was confirmed both in vitro and in vivo. The molecular mechanism of cDOPEY2 was investigated by mass spectrum, immunoprecipitation and ubiquitination analyses.Results: We report that a novel circRNA (cDOPYE2, hsa_circ_0008078) was markedly downregulated in cisplatin-resistant ESCC cells (ESCC-CR) compared with parental chemosensitive cells. Re-expression of cDOPEY2 substantially enhanced the cell-killing ability of cisplatin by augmenting the apoptotic process in ESCC-GR cells, which was achieved by decreasing the abundance of the antiapoptotic protein Mcl-1. Mechanistically, we showed that cDOPEY2 acted as a protein scaffold to enhance the interaction between the cytoplasmic polyadenylation element binding protein (CPEB4) and the E3 ligase TRIM25, which in turn facilitated the ubiquitination and degradation of CPEB4. The increased Mcl-1 expression in ESCC-GR cells was dependent on the binding of CPEB4 to its untranslated mRNA, and depletion of CPEB4 mediated by cDOPEY2 reversed this effect. Rescue experiments confirmed that the critical role of cDOPEY2 in maintaining cisplatin sensitivity was dependent on the depletion of CEPB4 and its downstream target Mcl-1. Clinical and in vivo data further corroborated the significant relevance of cDOPEY2 to cisplatin responsiveness in ESCC. Conclusions: We provide evidence that cDOPEY2 inhibits CPEB4-mediated Mcl-1 translation by promoting the ubiquitination and degradation of CPEB4 to alleviate cisplatin resistance, indicating that cDOPEY2 may serve as a valuable biomarker and potential therapeutic target in ESCC.
An Integrated Approach to Produce a Recombinant Yeast Pichia Pastoris For GLA Production
Authors: Firoozabad Maryam Sadat Mirbagheri , Akhbariyoon Hamidreza 
Published: July 2021

Abstract
γ-Linolenic acid (GLA) is an important n-6 polyunsaturated fatty acid (PUFA) used in many nutritional and medicinal applications such as the treatment of cancer, inflammatory disorders, and diabetes. However, GLA level of the total fatty acids in plant seeds and nuts as prominent sources of GLA is not enough to utilize on an industrial scale. The study aimed to improve the expression of delta-6 desaturase, which is one of the important enzymes in GLA production pathway. The expression vector pPICZC was selected for clone M.rouxii delta-6 desaturase. The engineered vector was first cloned into E. coli DH5α and after plasmid extraction and confirmation of sequencing was transformed by electroporation into Pichia pastoris GS115. The results indicated that the recombinant yeast strain expressed the gene in the presence of methanol 0.5%. The lipids and essential fatty acids especially GLA were extracted to confirm the expression. The results of studies of lipid and fatty acid production by Sudan black and Nile red staining, GC, and flow cytometry revealed that recombinant strain can produce GLA levels up to 19.2% of total fatty acids. The present study may provide an opportunity for the development of an alternative host for manufacturing GLA on an industrial scale.
Microinjection of Antisense Oligonucleotides into Living Mouse Testis Enables LncRNA Function Study
Authors: Chen Zhaohui , Ling Li , Shi Xiaolian , Li Wu , Zhai Huicong , Kang Zhenlong , Zheng Bangjin , Zhu Jiaqi , Ye Suni , Wang Hao , Tong Lingxiu , Huang Chaoyang , Li Yang , Zheng Ke 
Published: July 2021

Abstract
BackgroundLong non-coding RNAs (lncRNAs) has been the focus of ongoing research in a diversity of cellular processes. LncRNAs are abundant in mammalian testis, but their biological functions remain poorly known.ResultsHere, we established an antisense oligonucleotides (ASOs)-based targeting approach that can efficiently knock down lncRNA in living mouse testis. We cloned the full-length transcript of lncRNA Tsx (testis-specific X-linked) and defined its testicular localization pattern. Microinjection of ASOs through seminiferous tubules in vivo significantly lowered the Tsx levels in both nucleus and cytoplasm. This effect lasted no less than 10 days, conducive to the generation and maintenance of phenotype. Importantly, ASOs performed better in depleting the nuclear Tsx and sustained longer effect than small interfering RNAs (siRNAs). In addition to the observation of an elevated number of apoptotic germ cells upon ASOs injection, which recapitulates the documented description of Tsx knockout, we also found a specific loss of meiotic spermatocytes despite overall normal process of meiosis.ConclusionsOur study detailed the characterization of Tsx and illustrates ASOs as an advantageous tool to functionally interrogate lncRNAs in spermatogenesis.
Study on a Horizontal Axial Flow Pump during Runaway Process with Bidirectional Operating Conditions
Authors: Kan Kan , Zhang Qingying , Xu Zhe , Chen Huixiang , Zheng Yuan , Zhou Daqing , Binama Maxima 
Published: July 2021

Abstract
The ultra-low head pump stations often have bidirectional demand of water delivery, so there is a risk of runaway accident occurring in both conditions. To analyze the difference of the runaway process under forward runaway condition (FRC) and backward runaway condition (BRC), the whole flow system of a horizontal axial flow pump is considered. The Shear-Stress Transport (SST) k-ω model is adopted and the volume of fluid (VOF) model is applied to simulate the water surface in the reservoirs. Meanwhile, the torque balance equation is introduced to obtain the real time rotational speed, then the bidirectional runaway process of the pump with the same head is simulated. Additionally, the vortex transport equation is proposed to compare the contribution of vortex stretching and vortex dilatation terms. According to the changing law of the impeller torque, the torque curve can be divided into five stages: the drop, braking, rising, convergence and runaway stages. By comparison, the rising peak value of torque under FRC is significantly higher than that under BRC in the rising stage. Simultaneously, through the short time Fourier transform (STFT) method, the amplitude of torque pulsation is obviously different between FRC and BRC. The analysis reveals that the flow impact on blade surface increases the pressure difference between the two sides of the blade in braking condition, which leads to the torque increase in the rising stage. Moreover, the pulsation amplitude of torque is mainly affected by the integrity of the vortex rope.
Significantly Enhanced Coupling Effect and Gap Plasmon Resonance in a MIM-cavity based Sensing Structure
Authors: Chau Yuan-Fong Chou , Ming Tan Yu , Chao Chung-Ting Chou , Thotagamuge Roshan , Kooh Muhammad Raziq Rahimi , Huang Hung Ji , Lim Chee Ming , Chiang Hai-Pang 
Published: July 2021

Abstract
Herein, we design a high sensitivity with a multi-mode plasmonic sensor based on the square ring-shaped resonators containing silver nanorods together with a metal-insulator-metal bus waveguide. The finite element method is used to analyze the transmittance properties and electromagnetic field distributions of the structure in detail. Results show that the coupling effect between the bus waveguide and the side-coupled resonator can enhance by generating gap plasmon resonance among the silver nanorods, increasing the cavity plasmon mode in the resonator. The suggested structure obtained relatively high sensitivity and acceptable figure of merit and quality factor of about 2473 nm/RIU (refractive index unit), 34.18 1/RIU and 56.35, respectively. The achieved plasmonic sensor is ideal for lab-on-chip in gas and biochemical analysis and can significantly enhance the sensitivity by 177% compared to the regular one. The designed structure can apply in nanophotonic devices, and the range of the detected refractive index is suitable for gases and fluids (e.g., gas, isopropanol, optical oil and glucose solution).
Effects of Viscous Dissipation and Joule Heating on Micropolar Hybrid Nanofluid in a Stretching/Shrinking Channel including Thermal/Solar Radiation
Authors: El-dawy H.A , El-Amin Mohamed 
Published: July 2021

Abstract
This study aims to investigate the behavior of the micropolar hybrid nanofluid (Cu-TiO2) in a stretching and shrinking channel while maintaining a continuous laminar flow. External effects from magnetic field, thermal and solar radiation are also considered in the model. The governing differential (momentum, micro-rotation, and energy) equations are transformed into dimensionless forms via similarity transformations based on the boundary layer theory. The resulting ordinary differential equations are solved numerically using the fourth-order Runge-Kutta-Gill technique with the shooting method. The effects of nanoparticles transport on fluid flow and heat transfer are discussed and compared to the pure water case. The results are presented in tables or graphs such as velocity, temperature, angular-velocity profiles, and concentration.
The impact of RMD on urban runoff cannot be ignored in urban hydrologic management
Authors: Liu Yesen , huang yaohuan , liu yuanyuan 
Published: July 2021

Abstract
Urban floods have been exacerbated globally, associated with increasing spatial-temporal variations of rainfall. However, compared with rainfall variabilities of intensity and duration, the effect of RMD is always ignored. Based on 1313 rainfall scenarios with different combinations of rainfall intensity(RI) and RMD in a typical rainy city of Shenzhen in China, we investigate the urban runoff response in relation to RMD and the spatial feature of rivers. Our results show that the effect of RMD on the peak runoff may reach up to 20%, which will decrease to less than 5% under heavy RI conditions. In addition, we find that the impact of RMD is almost symmetrical and is associated with the direction of the river. The closer RMD is to the Linear Directional Mean of upstream, the larger peak runoff of section is. Our results reveal that RMD is significant to urban peak runoff in the downstream reaches, which should be considered in urban hydrologic model.
Stratified Assessment and Warning Regimen for Comprehensive Risk Management and Improved Iodinated Contrast Media Safety
Authors: Li Xue , Liu Heng , Qiu Haiyan , Wang Lingru , Liu Junling , Zhao Li , Wang Yaling , Zhang Weiguo 
Published: July 2021

Abstract
Objectives: To determine the relationship between stratified assessment and warning (SAW) regimen for risk factors to iodinated contrast media (ICM) and adverse drug reactions (ADR) occurrence.Methods: This retrospective study included patients who underwent enhanced CT examinations in our hospital between January 2014 and March 2016 (conventional assessment group) and between April 2017 and December 2019 (SAW group). Initial risk assessment was performed by clinicians when ordering examinations in both periods. The risk re-assessment was performed by simple question-and-answer onsite just before examination in the conventional assessment period, according to the risk factors that deserve special attention in ICM guidelines. In the SAW period, the re-assessment process prior to examination was moved forward as early as possible post-appointment, and a whole-process comprehensive management integrating risk identification, stratification, early warning and prevention was performed according different risk levels. Chi-square test was performed for comparisons of rates.Results: A total of 120822 cases in the conventional assessment group and 150343 cases in the SAW group were enrolled. The total ADR incidence in the SAW period (414/150343, 0.275%) was lower than that in the conventional assessment period (506/120822, 0.419%, P<0.001), in which the proportion of patients who developed ADR decreased by about 34%. It mainly presented as decrease in mild/moderate reactions (P<0.001) and increase in the proportion of mild ADR patients (P=0.001) in the SAW period. Subgroup analysis showed lower mild/moderate ADR incidence in patients with different risk levels and with different ICM injection parameters (P<0.05) following SAW regimen.Conclusions: SAW regimen was associated with lower mild/moderate ADR incidence and alleviated ADR severity in at-risk patients, which held potential for improved risk management and ICM safety.
Reusing System of the ballast water
Authors: Mounir Kirollos 
Published: July 2021

Abstract
Increasing water consumption is a problem faced by the world; one of its solutions is reusing water. Reuse is about changing processes that result in polluted water byproducts so that the resulting water is usable again. Our major problem is to reuse unusable or polluted water. We are not treating the water, but we are changing processes to make water byproducts usable again in the same process or even a different process. Our solution is to reuse ballast water in transporting oil. Oil ships are heading from one country to another, and they are loaded with oil. These ships return to be loaded with ballast water instead of oil to prevent the risk of overturning this ship. In other words, the ballast water is used to make the ship stable which is the purpose of the ballast water to return to their original countries in peace. Our goal is to purify this ballast water mixed with oil residues by relying on the density difference. The first step is to place a source of solid air in the tanks to provide some bubbles inside the tank. Secondly, put some of the iron peels inside the tanks to stick these peels with the bubbles, then by a magnetic field attracts the pollutant bubbles attached to the iron scales, and the ballast water remains in the tank to be used again. Before writing a test plan, we demonstrated two design requirements. The first one is ballast water quality which has two parts the turbidity and oil, grease, and all extractable matter by chloroform in the water, while the second design requirement is the environmental impact. By making a test, the results were astounding because it achieved the design requirements that we put into making this project applicable for reusing this polluted water after purifying it.