bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effects of essential oils obtained from wild and cultured forms of thyme (Origanum acutidens) on lung cancer cells membrane
Authors: GÖKHAN Ayşe 
Published: July 2021

Aim: Nowadays, studies on the use of medicinal plants and their essential oils in cancer treatment are increasing rapidly and these studies are very important both scientifically and economically. However, the collection of plants from nature causes extinction. Therefore, it is of great importance to cultivate plants, especially endemic species, to reproduce and consume them. In our study, cytotoxic and membrane damaging effects of essential oils obtained from wild and cultured forms of Origanum acutidens (Hand.-Mazz.) Ietswaart (Lamiaceae) (O. acutidens), which is endemic to Turkey, on non-small-cell lung cancer (NSCLC) cells H1299 and A549 were compared.
Material and Method: The level of malondialdehyde, an oxidative stress biomarker, was determined in cell lysates. Assessment of cell viability was made by CellTiter-Blue® Cell Viability Assay and 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazoliumbromide (MTT) assay after 10- 250 μg/mL concentrations of wild and cultured forms of O. acutidens essential oil treated to H1299 and A549 cells for 24, 48 and 72 h. Malondialdehyde levels were assayed for determining the membrane damaging effects.
Results: Cell viability of H1299 and A549 cells incubated with essential oils obtained from wild and cultured forms of O. acutidens was found to decrease depending on concentration and time. 179 µg/mL, 157 µg/mL, and 132 µg/mL were calculated as IC50 values of wild form of O. acutidens essential oil on H1299 cells for 24, 48, and 72 h, respectively by MTT assay. 150 µg/mL, 131 µg/mL, and 110 µg/mL were calculated as IC50 values of wild form of O. acutidens essential oil on H1299 cells for 24, 48, and 72 h, respectively by resazurin-based assay. 118 µg/mL, 99 µg/mL, and 69 µg/mL were calculated as IC50 values of wild form of O. acutidens essential oil on A549 cells for 24, 48, and 72 h, respectively by MTT assay. 98 µg/mL, 83 µg/mL, and 57 µg/mL were calculated as IC50 values of wild form of O. acutidens essential oil on A549 cells for 24, 48, and 72 h, respectively by resazurin-based assay. Essential oils obtained from wild and cultured forms of O. acutidens increased malondialdehyde level on both H1299 and A549 cells. The greatest membrane damage was observed in A549 cells treated with wild form of O. acutidens essential oil.
Conclusion: Essential oils obtained from wild and cultured forms of O. acutidens had cytotoxic effect on lung cancer cells and it has been demonstrated that they showed this effect by causing membrane damage in cells.
Laboratory parameters can be used to differentiate renal infarction and urolithiasis in patients who are admitted to the emergency department with flank pain
Authors: ÇETİNKAYA Hasan Basri , ALATLI Tufan 
Published: July 2021

Abstract
Aim
We aimed to determine the predictive value of laboratory parameters for the distinction between urolithiasis (UL) and renal infarction (RI) in patients presenting to the emergency department with flank pain complaint.
Methods
In our retrospective study, the files of 73 patients who presented to the emergency department (ED) with flank pain and whose costovertebral angle tenderness was positive were reviewed. Routine blood tests and the results of contrast-enhanced abdominal computed tomography were obtained. The patients were divided into two groups as RI and UL according to the results of computed tomography. Accordingly, 8 patients were found to have RI, and 65 had UL.
Results
LDH and PLR values were significantly higher in the RI group compared to the UL group (p
Evaluation of serum adropin and irisin levels and its association with anthropometric obesity indices and biochemical parameters in Type 2 diabetic patients
Authors: Alzoughool Foad , Al Hourani Huda , Atoum Manar , Bateineh Sajedah , Abu shaikh Hanan , Al-Zghool Hamza , Al-Shudifat Abdel-ellah 
Published: July 2021

BACKGROUND/AIM: The newly described proteins adropin and irisin are a highly conserved polypeptide that plays essential roles in metabolic and energy homeostasis, insulin resistance, and fat browning. The aim of this study is to evaluate the circulating levels of serum adropin and irisin in type 2 diabetes mellitus (T2DM) patients and also to elucidate possible relationships between serum adropin and irisin levels with anthropometric obesity indices and biochemical parameters. SUBJECTS AND METHODS: Single-center prospective observational study included 90 T2DM patients referred to the diabetes outpatient clinic. Height, weight, and waist circumference (WC) were measured. Body mass index (BMI) and waist to height ratio (WHtR) were calculated. Fasting blood glucose, glycosylated hemoglobin, serum lipids, creatinine, urea, and blood urea nitrogen were evaluated. Estimated glomerular filtration rate (GFR) was calculated, serum adropin and irisin were evaluated. RESULTS: The results showed a significant positive correlation between adropin and irisin in females but not in males ( r = 0.311; P = 0.042). In males’ group, serum adropin levels showed significant negative correlation with serum glucose (–0.423, P = < 0.05), HbA1C (–0.364, P = < 0.05), and GFR (–0.355, P = < 0.05). In contrast, creatinine was showed a significant positive correlation with adropin in males (0.381, P = < 0.05). In females’ group, adropin showed a significant negative correlation with weight (–0.371, P = < 0.05), BMI (–0.349, P = < 0.05), WC (–0.402, P = < 0.01), and WHtR (–0.398, P = < 0.01). Contrary, in males’ group, serum irisin levels showed significant positive correlation with weight (0.338, P = < 0.05), BMI (0.332, P = < 0.05), WC (0.409, P = < 0.01), and WHtR (0.432, P = < 0.01). CONCLUSION: This study demonstrated that, in T2DM patients, circulating serum adrpoin correlated negatively with anthropometric obesity indices of obesity in females, while serum irisin was positively correlated with anthropometric obesity indices of obesity in males.
THE OROPHARYNX MICROBIOME PROFILE OF CHILDREN WITH INFECTIOUS MONONUCLEOSIS EPSTEIN-BARR VIRAL ETIOLOGY
Authors: Nadraga A.B. , Korniychuk O.P. , Klymenko Kh.P. 
Published: July 2021

Relevance. The microbiota of the oral cavity is individual and the consistency of its composition provides a protective function that is regulated by the immune system. Dysbiotic changes in the oral microbiota composition lead to a devastation of local immunity and provide to the disease's development. Herpes virus infection аctivation suppresses the immune response which increases the level of oral mucosa bacteria colonization in particular during the acute course of infectious mononucleosis (IM).
Objective: to study the features of the oral microbiome composition and its sensitivity to antibacterial drugs in patients with infectious mononucleosis and acute tonsillitis.
Materials and methods. We examined 306 children aged 7 to 236 months. The main group (n = 280) – children with IM. Comparison group (n = 26) – children diagnosed with acute tonsillitis. The main group was divided into two subgroups: subgroup I – 234 children with MI caused by the Epstein-Barr virus (EBV), and subgroup II – 46 children with co-infection with the EBV virus and cytomegalovirus (CMV). The examination was performed according to the treatment protocol: general and biochemical analysis of blood, determination of IgM antibodies to Epstein-Barr virus and cytomegalovirus, bacteriological examination of oropharyngeal mucus, followed by determination of the sensitivity of the identified pathogens to antibacterial drugs.
The results. In oral microbiome of children with IM caused by EBV and CMV co-infection pneumococcus dominant. In children with acute tonsillitis, Staphylococcus aureus, S. pyogenes infection, and yeast-like fungi were predominant. S. pyogenes, in children with co-infection, has a high level of resistance to β-lactam antibiotics. Higher levels of antibiotic-resistant strains were found among Enterococcus spp. Isolates, especially in children with EBV-induced MI and co-infection of EBV and CMV.
Conclusions. The development of MI is accompanied by microecological dysbiosis of the mucous membranes of the oropharynx and tonsils, which do not provide enough level of colonization resistance, contributing to the spread of antibiotic resistance among resident and transient microbiota.
Comparative analysis of hypotonic solutions for cytogenetic studies of animals
Authors: Novgorodova Inna P. 
Published: July 2021

Relevance. Cytogenetic studies are in demand to identify carriers of hereditary anomalies. In order to obtain metaphase plates of the chromosomes of various animal species, we optimized the usual method, taking into account some adjustments.Methods. The main research criterion was the selection of a hypotonic solution for cytogenetic studies in sheep and goats. 3 variants of using hypotonic solutions were considered: 0.56% solution of potassium chloride; distilled water and 0.56% solution of potassium chloride; 2% solution of sodium citrate and 0.56% solution of potassium chloride (1:1). For the cultivation of peripheral blood cells, blood was taken from the jugular vein of animals (goats n = 8, sheep n = 11).Results. The cells were cultured according to the generally accepted method. The results of the conducted studies showed that the quality of the preparations differed depending on the components used. The optimal parameters for the preparation of chromosomes with a good distribution were obtained by culturing sheep lymphocyte cells using 0.56% potassium chloride as a hypotonic solution (20 min) and ranged from 14.29 to 25.00%. For cytogenetic studies in goats, optimal results were found when using a 2% solution of sodium citrate with a 0.56% solution of potassium chloride (1:1) for 20 minutes — from 13.33 to 25.00%. Each option allowed to reduce the loss of cells during the preparation of chromosome preparations for a certain type of animal. Thus, the developed protocol for obtaining metaphase plates using a 2% solution of sodium citrate with a 0.56% solution of potassium chloride (1:1) as a hypotonic solution is optimal when used in goats, while the classical method (0.56% solution of potassium chloride) was most suitable for sheep.
- Manajemen Ekstrakurikuler Seni Batik untuk Mengembangkan Potensi Diri dan Melestarikan Warisan Budaya Takbenda Indonesia
Authors: Sari Erna Kartika 
Published: July 2021

Tujuan penelitian adalah (1) Mengetahui strategi manajemen ekstrakurikuler seni batik untuk mengembangkan potensi diri peserta didik. (2) Mengetahui strategi manajemen ekstrakurikuler seni batik untuk melestarikan Warisan Budaya Takbenda Indonesia pada peserta didik.
Jenis penelitian yang digunakan adalah kualitatif deskriptif dengan menggunakan tehnik pengumpulan data melalui wawancara, observasi dan dokumentasi. Proses analisis data dilakukan dengan mencheck list data, menjustifikasi kelayakan data dan verifikasi data (menarik kesimpulan). Keabsahan data dilakukan melalui metode member checking, triangulasi, perpanjangan keikutsertaan, ketekunan observasi dan kajian kasus negatif.
Hasil Penelitian ini adalah (1) Strategi manajemen ekstrakurikuler seni batik yang dikelola dengan baik dan sistematis dapat mengembangkan potensi diri peserta didik. (2) Strategi manajemen ekstrakurikuler seni batik yang dikelola dengan baik dan sistematis dapat memberikan kontribusi pada pelestarian Warisan Budaya Takbenda Indonesia.
Odontología de mínima intervención: minimizando el ciclo de la restauración: Revisión de la literatura
Authors: Naccha Torres Andres , Otazú Aldana Claudia Gabriela 
Published: July 2021

Objective: to present the current concepts of Minimal Intervention Dentistry with an emphasis on minimizing the restoration cycle. Materials and methods: a bibliographic search of articles in English and Spanish was carried out in databases: Pubmed, with the words: dental caries, minimal invasive dentistry; Also, Google Scholar search was used with the keywords: dental caries, minimal intervention dentistry, tooth structure and dental remineralization in articles published between 2016 and 2020. Results: the sample consisted of 40 scientific publications, including a literature review, systematic reviews and meta-analysis, case reports, complete original articles referring to deciduous and mixed dentition, and minimal intervention treatment alternatives. Conclusions: the objective of Minimal Intervention Dentistry is to avoid unnecessarily removing healthy dental tissue, avoiding the use of the high-speed handpiece and reducing the restoration cycle, allowing to reduce work time, stress for pediatric patient and placement of anesthesia. Treatment alternatives within the MID premise are shown to dentists so they can use them in their pediatric patients according to the clinical cases that are presented to them.
OBEYING TO KYAI FOR MARRIAGE: A TRADITION IN MAQĀSID SHARĪ’AH PERSPECTIVE
Authors: Masruh Ahmad 
Published: July 2021

This article is the result of a qualitative field research. The research location is at the At-Tahdzib Islamic Boarding School Rejoagung Ngoro Jombang. The direction of the discussion in this article is how to place the marriage tradition following the Kyai within the framework of maqasid marriage and maqasid shariah. The researcher uses maqasid al-nikah and maqasid shariah theory in explaining the position of the marriage tradition following the Kyai, because the maqasid al-nikah and maqasid shariah theories. The researcher argues that the marriage tradition following the Kyai provides advocacy for the protection of the bride and groom in achieving the goals of marriage and the goals of the Shari'a that arise from marriage
Keywords: marriage is obedient to the kyai, maqasid shariah.

Abstrak
Artikel ini adalah hasil penelitian kualitatif lapangan. Lokasi penelitian berada di Pesantren At-Tahdzib Rejoagung Ngoro Jombang. Arah diskusi artikel ini adalah bagaimana mendudukkan tradisi pernikahan mengikuti Kyai dalam bingkai maqasid pernikahan dan maqasid shariah. Peneliti menggunakan maqasid al-nikah dan teori maqasid shariah dalam menjelaskan posisi tradisi pernikahan mengikuti Kyai, karena teori maqasid al-nikah dan maqasid shariah. Peneliti berargumen bahwa tradisi pernikahan mengikuti Kyai memberikan advokasi perlindungan kepada calon pengantin dalam mencapai tujuan pernikahan dan tujuan syariat yang timbul akibat pernikahan.
Kata Kunci: pernikahan taat kyai, maqasid shariah,
Emodin Prevented Depression in Chronic Unpredicted Mild Stress-Exposed Rats by Targeting miR-139-5p/5-Lipoxygenase
Authors: Zhang Teng , Yang Can , Chu Jiang , Ning Lin-Na , Zeng Peng , Wang Xiao-Ming , Shi Yan , Qin Bao-Jian , Qu Na , Zhang Qi , Tian Qing 
Published: July 2021

BackgroundThe use of medicinal plant ingredients is one of the goals of developing potential drugs for treating depression. Compelling evidence suggests that anti-inflammatory medicines may block the occurrence of depression. We studied the effect of a natural compound, emodin, on the development of psychosocial stress-induced depression and the underlying mechanisms.MethodsChronic unpredicted mild stress (CUMS) for 7 weeks was performed to replicate psychosocial stress in rats. The sucrose preference test, force swimming test, and open field test were used to evaluate their behaviors. The differentially expressed proteins in the hippocampus were analyzed using proteomics. Nissl staining and Golgi staining were used to detect the loss of neurons and synapses, immunohistochemical staining was used to detect the activation of microglia, and the enzyme-linked immunosorbent assay was used to detect the levels of pro-inflammatory cytokines. Western blotting, immunofluorescence, and quantitative polymerase chain reaction were also performed.ResultsHippocampal inflammation with up-regulated 5-lipoxygenase (5-LO) was observed in the depressed rats after CUMS exposure. The upregulation of 5-LO was caused by decreased miR-139-5p. To observe the effect of emodin, we screened out depression-susceptible (DeS) rats during CUMS and treated them with emodin (80 mg/kg/day). Two weeks later, emodin prevented the depression behaviors in DeS rats along with a series of pathological changes in their hippocampi, such as loss of neurons and spines, microglial activation, increased interleukin-1β and tumor necrosis factor-α, and the activation of 5-LO. Furthermore, we demonstrated that emodin inhibited its excess inflammatory response, possibly by targeting miR-139-5p/5-LO and modulating glycogen synthase kinase 3β and nuclear factor erythroid 2-related factor 2.ConclusionThese results provide important evidence that emodin may be a candidate agent for the treatment of depression and established a key role of miR-139-5p/5-LO in the inflammation of depression.
Inability of Low Oxygen Tension to Induce Chondrogenesis in Human Infrapatellar Fat Pad Mesenchymal Stem Cells
Authors: Rahman Samia , Szojka Alexander R. A. , Liang Yan , Kunze Melanie , Goncalves Victoria , Mulet-Sierra Aillette , Jomha Nadr M. , Adesida Adetola B. 
Published: July 2021

ObjectiveArticular cartilage of the knee joint is avascular, exists under a low oxygen tension microenvironment, and does not self-heal when injured. Human infrapatellar fat pad-sourced mesenchymal stem cells (IFP-MSC) are an arthroscopically accessible source of mesenchymal stem cells (MSC) for the repair of articular cartilage defects. Human IFP-MSC exists physiologically under a low oxygen tension (i.e., 1–5%) microenvironment. Human bone marrow mesenchymal stem cells (BM-MSC) exist physiologically within a similar range of oxygen tension. A low oxygen tension of 2% spontaneously induced chondrogenesis in micromass pellets of human BM-MSC. However, this is yet to be demonstrated in human IFP-MSC or other adipose tissue-sourced MSC. In this study, we explored the potential of low oxygen tension at 2% to drive the in vitro chondrogenesis of IFP-MSC. We hypothesized that 2% O2 will induce stable chondrogenesis in human IFP-MSC without the risk of undergoing endochondral ossification at ectopic sites of implantation.MethodsMicromass pellets of human IFP-MSC were cultured under 2% O2 or 21% O2 (normal atmosphere O2) in the presence or absence of chondrogenic medium with transforming growth factor-β3 (TGFβ3) for 3 weeks. Following in vitro chondrogenesis, the resulting pellets were implanted in immunodeficient athymic nude mice for 3 weeks.ResultsA low oxygen tension of 2% was unable to induce chondrogenesis in human IFP-MSC. In contrast, chondrogenic medium with TGFβ3 induced in vitro chondrogenesis. All pellets were devoid of any evidence of undergoing endochondral ossification after subcutaneous implantation in athymic mice.
Oseltamivir Improved Thrombocytopenia During Veno-Arterial Extracorporeal Membrane Oxygenation in Adults With Refractory Cardiac Failure: A Single-Center Retrospective Real-World Study
Authors: Li Yuan , Wang Lin , Zhang Jianning , Han Hui , Liu Han , Li Chaoyang , Guo Haipeng , Chen Yuguo , Chen Xiaomei 
Published: July 2021

Background: Severe thrombocytopenia is a common complication of extracorporeal membrane oxygenation (ECMO). Oseltamivir can be used to treat infection-associated thrombocytopenia.Objective: To evaluate the effect of oseltamivir on attenuating severe thrombocytopenia during ECMO.Methods: This was a single-center real-world study in critically ill patients supported with venous-arterial extracorporeal membrane oxygenation (VA-ECMO). Patients suspected or confirmed with influenza received oseltamivir according to the Chinese guidelines. Thrombocytopenia and survival were compared between the oseltamivir-treated and untreated group. The factors associated with survival were analyzed by multivariable Cox analysis.Results: A total of 82 patients were included. All patients developed thrombocytopenia after initiating VA-ECMO. Twenty-three patients received oseltamivir (O+ group), and 59 did not use oseltamivir (O− group). During the first 8 days after VA-ECMO initiation, the platelet count in the O+ group was higher than that in the O− group (all P &lt; 0.05). The patients in the O+ group had a higher median nadir platelet count (77,000/μl, 6,000–169,000/μl) compared with the O− group (49,000/μl, 2,000–168,000/μl; P = 0.04). A nadir platelet count of &lt;50,000/μl was seen in 26% of the patients in the O+ group, compared with 53% in the O− group (P = 0.031). No significant difference in survival from cardiac failure was seen between the O+ and O− group (48 vs. 56%, P = 0.508). The Sequential Organ Failure Assessment (SOFA) score on initiation of VA-ECMO were independently associated with survival (OR = 1.12, 95% confidence interval (95% CI): 1.02–1.22, P = 0.015).Conclusions: Oseltamivir could ameliorate VA-ECMO-related thrombocytopenia. These findings suggested the prophylactic potential of oseltamivir on severe thrombocytopenia associated with the initiation of VA-ECMO.
Non-invasive CMR-Based Quantification of Myocardial Power and Efficiency Under Stress and Ischemic Conditions in Landrace Pigs
Authors: Faragli Alessandro , Alogna Alessio , Lee Chong Bin , Zhu Miry , Ghorbani Niky , Lo Muzio Francesco Paolo , Schnackenburg Bernhard , Stehning Christian , Kuehne Titus , Post Heiner , Goubergrits Leonid , Nagel Eike , Pieske Burkert , Kelle Sebastian , Kelm Marcus 
Published: July 2021

Background: Myocardial efficiency should be maintained stable under light-to-moderate stress conditions, but ischemia puts the myocardium at risk for impaired functionality. Additionally, the measurement of such efficiency typically requires invasive heart catheterization and exposure to ionizing radiation. In this work, we aimed to non-invasively assess myocardial power and the resulting efficiency during pharmacological stress testing and ischemia induction.Methods: In a cohort of n = 10 healthy Landrace pigs, dobutamine stress testing was performed, followed by verapamil-induced ischemia alongside cardiac magnetic resonance (CMR) imaging. External myocardial power, internal myocardial power, and myocardial efficiency were assessed non-invasively using geometrical and functional parameters from CMR volumetric as well as blood flow and pressure measurements.Results: External myocardial power significantly increased under dobutamine stress [2.3 (1.6–3.1) W/m2 vs. 1.3 (1.1–1.6) W/m2, p = 0.005] and significantly decreased under verapamil-induced ischemia [0.8 (0.5–0.9) W/m2, p = 0.005]. Internal myocardial power [baseline: 5.9 (4.6–8.5) W/m2] was not affected by dobutamine [7.5 (6.9–9.0) W/m2, p = 0.241] nor verapamil [5.8 (4.7–8.8) W/m2, p = 0.878]. Myocardial efficiency did not change from baseline to dobutamine [21% (15–27) vs. 31% (20–44), p = 0.059] but decreased significantly during verapamil-induced ischemia [10% (8–13), p = 0.005].Conclusion: In healthy Landrace pigs, dobutamine stress increased external myocardial power, whereas myocardial efficiency was maintained stable. On the contrary, verapamil-induced ischemia substantially decreased external myocardial power and myocardial efficiency. Non-invasive CMR was able to quantify these efficiency losses and might be useful for future clinical studies evaluating the effects of therapeutic interventions on myocardial energetics.