bluuebunny/crossref_metadata_2025_split huggingface dataset:
941-P: Epiallopregnanolone Sulfate Is Reduced in Gestational Diabetes Mellitus and Induces Glucose-Stimulated Insulin Secretion
Authors: FAN HEI MAN , MITCHELL ALICE , GIORGI MIRKO , JONES PETER M. , MCCANCE DAVID R. , ANDERSSON DAVID A. , BEVAN STUART , MARSCHALL HANNS-ULRICH , EBERINI IVANO , FRATERNALI FRANCA , HUNT KATHARINE F. , BOWE JAMES , WILLIAMSON CATHERINE 
Published: June 2021

Introduction: Serum progesterone sulfate (P4S) concentrations increase in pregnancy and bind receptors that influence glucose homeostasis. We hypothesized that P4S modulate glucose homeostasis in pregnancy.
Methods: Serum P4S were assayed using ultra-performance liquid chromatography- tandem mass spectrometry. Samples were studied in three separate patient groups: women with GDM (n=20) and matched healthy controls (n=38); participants (average 28 weeks’ gestation) from the upper (n=93) and lower (n=94) quartiles of fasting plasma glucose in the Hyperglycemia and Adverse Pregnancy Outcomes (HAPO) Study; 11-13 week pregnant women with BMI≤25 or BMI≥35 who subsequently had uncomplicated pregnancies or developed GDM (n=50/group). Glucose-stimulated insulin secretion (GSIS) was quantified in isolated wild-type, Fxr-/- and Tgr5-/- mouse and human islets in the presence of P4S and TRPM3 inhibitor isosakuranetin (ISO). Intracellular calcium concentrations were measured after treatment with P4S in TRPM3 transfected HEK293. Computer modelling using Molecular Operating Environment generated 3D structures of TRPM3 and P4S binding.
Results: Epiallopregnanolone sulfate (PM5S) concentrations were reduced in serum samples from the HAPO study participants with higher fasting plasma glucose (p&lt;0.01), in women with GDM (p&lt;0.05) and in early pregnancy samples from women with BMI ≥35 who subsequently developed GDM (P&lt;0.05). In wild-type and human islets, 50μM PM5S increased GSIS by at least 2-fold at 20mM glucose concentrations (P&lt;0.001). This effect was not abolished from islets obtained from Fxr-/- or Tgr-/- mice, however it was abolished by ISO. PM5S elicited Ca2+ influx in TRPM3-expressing HEK293 cells. Computer modelling and docking showed identical positioning of PM5S to cholesterol hemisuccinate in TRPM3.
Conclusion: PM5S increases GSIS and concentrations are reduced in the serum of women with GDM. The increased GSIS is mediated by TRPM3.

Disclosure
H. Fan: None. F. Fraternali: None. K. F. Hunt: None. J. Bowe: None. C. Williamson: Consultant; Self; GlaxoSmithKline plc., Mirum Pharmaceuticals. A. Mitchell: None. M. Giorgi: None. P. M. Jones: None. D. R. Mccance: None. D. A. Andersson: None. S. Bevan: None. H. Marschall: None. I. Eberini: None.

Funding
Tommy’s; Guy’s and St Thomas’ Biomedical Research Centre; National Health Service
943-P: Glycated Albumin as a Predictor of Adverse Neonatal Events in Pregnant Women with Diabetes
Authors: POWERS CARSON JENNIFER L. , CARTER EBONY B. 
Published: June 2021

Control of plasma glucose is effective to reduce adverse neonatal events (ANE) for pregnant women with diabetes, including gestational diabetes (GDM); however, compliance can be poor. We examined the utility of glycated serum protein (GSP) and glycated albumin (%GA) to predict risk of ANE. Published studies have shown greater risk if %GA of ≥ 12 -15.8%, but population and trimester-specific cut-offs are needed. Additionally, BMI has been reported to affect %GA. We prospectively recruited pregnant women with type 2 diabetes or GDM and collected serum during the second and/or third trimesters. No exclusion criteria were used, but an attempt was made to recruit similar numbers of overweight and Class I, II, or III obese BMI. Enrolled subjects included 52% African-American and 41% Caucasian with median BMI 34.0 (25.0 - 70.4). Thirty bio-banked samples, matched for ethnicity, BMI and gestational age, from women with no evidence of diabetes were used as controls. GSP and %GA as well as various lipid markers were measured using methods from Roche or Diazyme (GSP, %GA) on automated analyzers. Our goals were to determine preliminary cut-off values for GSP and %GA to predict ANE and identify whether additional lipid markers may improve prediction. Of those delivered to date (n=55), two controls and four subjects experienced one or more ANE (macrosomia, hyperbilirubinemia, hypoglycemia or RSD). Using receiver operator curve analysis, trimester two values of %GA or GSP were much better at predicting ANE (AUC: 0.896, 0.910, respectively) than trimester three values (AUC: 0.662, 0.713, respectively). Our data suggests that a cut-off ≥ 11.8 %GA can effectively predict those at greatest risk in this population. Statistical analysis showed no differences in %GA or GSP between ethnicities or BMI subgroups. Additional data is needed to determine whether or not incorporation of lipid markers will improve risk prediction. However, only beta-hydroxybutyrate shows significant differences between subjects and controls.

Disclosure
J. L. Powers carson: None. E. B. Carter: None.

Funding
American Diabetes Association/Pathway to Stop Diabetes (1-19-ACE-02 to E.B.C.); National Institutes of Health (P30DK020579)
96-OR: Parameters of Glycemic Variability as Predictors of LGA in Pregnant Women with Well-Controlled Type 1 Diabetes (T1D)
Authors: SIBIAK RAFAL , MRZEWKA-ROGACZ BEATA , MANTAJ URSZULA , GUTAJ PAWEL , WENDER-OZEGOWSKA EWA 
Published: June 2021

Background: According to the current recommendations, pregnant patients with T1D are encouraged to use continuous glucose monitoring (CGM) systems. The use of CGM reduces the risk of LGA births and neonatal hypoglycemia. This study aimed to assess the influence of the parameters of glycemic variability on the risk of LGA births.
Methods: The study cohort included 75 patients with T1D who were using CGM systems throughout pregnancy. The study population we divided into two subgroups: mothers of LGA and non-LGA newborns. We defined LGA as a birth weight above the 90th percentile. Calculated birthweight percentiles we adjusted for maternal weight, height, parity, and ethnicity, as well as the infant’s sex and gestational age. For each trimester separately and for the whole pregnancy, we calculated indicators of glucose variability, such as time spent in and above the target range (TIR, TAR). We also calculated the %CV, MODD, MAGE, GRADE, %GRADE hypo, %GRADE hyper, CONGA (1, 2h), LBGI, and HBGI.
Results: The mean values of glycated haemoglobin in both groups met the criteria of well-controlled T1D in each trimester of pregnancy. Nevertheless, there were 25/75 infants with LGA. Only the 1st-trimester maternal HbA1c values were significantly higher in the group of LGA infants. However, in the LGA-mothers, mean glucose levels and TAR values were significantly increased in each trimester. Bivariate logistic regression revealed that the LGA risk was positively associated with %GRADE hyper, MODD, MAGE, and TAR, and negatively associated with LBGI, %GRADE hypo, and TIR. After the adjustments for the maternal age, pregestational BMI, gestational weight gain, and HbA1c values, only the relationships with the first trimester’s %GRADE hypo and %GRADE hyper as well as %GRADE hypo calculated for whole pregnancy remained significant.
Conclusion: The parameters of glucose variability rather than HbA1c may help predict LGA infants, especially in patients with well-controlled diabetes.

Disclosure
R. Sibiak: None. B. Mrzewka-rogacz: None. U. Mantaj: None. P. Gutaj: None. E. Wender-ozegowska: Advisory Panel; Self; Novo Nordisk, Board Member; Self; Medtronic, Other Relationship; Self; Medtronic, Novo Nordisk.
97-LB: Bringing Simulation Technologies to People with T1D: A Pilot Study
Authors: COLMEGNA PATRICIO , BISIO ALESSANDRO , MCFADDEN RYAN , WAKEMAN CHRISTIAN A. , OLIVERI MARY C. , NASS RALF , BRETON MARC D. 
Published: June 2021

Background: Researchers have extensively used metabolic simulators as a fast, inexpensive, and safe way of testing novel treatment strategies. This work aims to bring this technology to people with T1D to enable unique patient/data interactions.
Methods: A 5-week pilot study was carried out in 15 adults with T1D using Control-IQ technology (age 36±13 years, HbA1c 6.5±0.7%) to evaluate acceptance of the proposed Web-Based Simulation Tool (WST). The study consisted of 1 week of observation (Phase 1) and 4 weeks of interaction with WST (Phase 2). Data were automatically collected via Tandem Diabetes Care t:connect web application, and used to generate personalized models of the participants’ glucose metabolism.
Results: Success rate in generating models was 86.4%, achieving an average MARD of 7.4±3.2%. Interaction time was 15.8±10.7 min per week. Comparing Phases 1 and 2, no variation was detected in time in range 70-180 mg/dl (80.1 [70.4,89.6]% vs. 80 [69.7,87.9]%). Time in 70-250 mg/dl increased slightly (94.2 [90.3,95.7]% vs. 96.2 [92.1,97.9]%), especially overnight (92.8 [88.3,98.2]% vs. 97.2 [91.8,99.6]%), and for participants who modified their pump settings based on WST simulations (90 [88.7,92.4]% vs. 94.5 [87.1,99.6]%). One subject tested COVID positive during the study and was excluded from this analysis due to abnormal hyperglycemia.
Analysis of Diabetes Distress Scale (DDS)-17 pre and post-system use shows a reduction in diabetes-related distress (2.2 [1.7,3.4] vs. 2 [1.7,2.4]). Trust, ease of use, and usefulness scores were 80 [60,80]%, 60 [60,80]%, and 80 [60,80]%, respectively. During the follow up interviews, 10 participants reported they enjoyed using WST and would implement it into their diabetes management; 2 did not like the system but see the potential of it for other people; 3 participants did not like the system at all.
Conclusions: Evidence from this study suggests that simulation technologies may empower people with T1D, making them more confident in their diabetes self-management.

Disclosure
P. Colmegna: None. A. Bisio: None. R. Mcfadden: None. C. A. Wakeman: Stock/Shareholder; Self; Dexcom, Inc., Tandem Diabetes Care. M. C. Oliveri: None. R. Nass: None. M. D. Breton: Consultant; Self; ADOCIA, Dexcom, Inc., Research Support; Self; Arecor, Dexcom, Inc., Novo Nordisk A/S, Tandem Diabetes Care, Speaker’s Bureau; Self; Arecor, Tandem Diabetes Care, Stock/Shareholder; Self; Dexcom, Inc., Insulet Corporation, Tandem Diabetes Care.

Funding
JDRF (2-APF-2019-737-A-N)
994-P: Prevalence and Persistence of Post-diagnosis Hyperglycemia in Cancer Patients
Authors: VISKOCHIL RICHARD , PAULECK SVENJA , WINN MACI , FUNAI KATSU , LITCHMAN MICHELLE L. , AKERLEY WALLACE , COLMAN HOWARD , ULRICH CORNELIA M. , PLAYDON MARY C. , HARDIKAR SHEETAL 
Published: June 2021

Introduction: Cancer patients are more likely to develop hyperglycemia (HG) compared to cancer-free adults, and this increased risk is highest in the year following cancer diagnosis. The persistence of post-diagnosis HG and degree of glycemic control in this population is unclear.
Methods: This study utilized data from n=9,588 cancer patients from the Huntsman Cancer Institute, UT. Clinicodemographic characteristics, biomarkers, and cancer diagnoses were extracted from electronic medical records (EMR) and tumor registry. Patients with a new HG diagnosis (ICD) of any kind between 30 days and 1 year after cancer diagnosis were included. Persistence of HG was determined through elevated Hemoglobin A1c (HbA1c) and/or new anti-HG medication prescription. Patients who did not survive &gt;1 year after cancer diagnosis or were diagnosed with cancer &lt;1 year prior to EMR abstraction were excluded.
Results: Of the 2,964 (30.9%) cancer patients with a HG diagnosis after cancer, 26.2% (n=777) were diagnosed with HG between 30 days and 1 year after cancer diagnosis, of these n=357 met criteria for HG evaluation. Data on HbA1c and/or anti-HG medication &gt;1 but &lt;2 years post-cancer diagnosis was available for 109 patients. Of these, 60 (55.0%) received a new anti-HG medication prescription and an additional 30 (27.5%) had an HbA1c greater than 5.7%, indicative of HG. Interestingly, body mass index (BMI) was similar among those that did and did not receive anti-HG medication (32.0 ± 6.4 vs. 32.6 ± 7.8 kg/m2, respectively), suggesting an alternate mechanism for lack of glycemic control.
Discussion: Taken together, 82.5% of patients diagnosed with HG in the year following a cancer diagnosis still presented with HG between one and two years post-cancer diagnosis, and there was no difference by BMI among those that did and did not receive anti-HG medications. This suggests that HG post-cancer diagnosis is not a transient effect of cancer therapy (e.g., corticosteroids), and may have long-term health implications in cancer survivors.

Disclosure
R. Viskochil: None. S. Hardikar: None. S. Pauleck: None. M. Winn: None. K. Funai: None. M. L. Litchman: Research Support; Self; Abbott Diabetes. W. Akerley: None. H. Colman: Advisory Panel; Self; Adastra Pharmaceuticals, Orbus Therapeutics, Consultant; Self; Best Doctors/Teladoc. C. M. Ulrich: None. M. C. Playdon: None.

Funding
University of Utah Health/Larry H. Miller Family Wellness Initiative
Translocation of the conus medullaris during dynamic lumbosacral magnetic resonance imaging in dogs
Authors: Nam Jihye , Kang Kyuyong , Kim Kyeonga , Choi Jeongwoo , Choi Mincheol , Yoon Junghee 
Published: June 2021

Abstract

OBJECTIVE
To investigate the change in the lumbosacral angle (ΔLSA) and conus medullaris (CM) displacement in healthy dogs undergoing dynamic MRI with changes in the posture of their pelvic limbs from neutral posture to flexion or extension posture and to evaluate for potential correlation between ΔLSA and CM displacement.

ANIMALS
9 healthy adult Beagles.

PROCEDURES
Dogs underwent dynamic MRI with their pelvic limbs positioned in neutral, flexion, and extension postures. From T2-weighted sagittal midline plane MRI images, 2 observers measured the lumbosacral angle and CM location in duplicate for each posture for each dog. Intra- and interobserver agreement was assessed, and the Spearman rank correlation coefficient (ρ) was used to assess for potential correlation between ΔLSA and CM displacement for changes in pelvic limb posture from neutral to flexion or extension.

RESULTS
Overall, the mean ΔLSA and CM displacement for changes in posture were 23° and 9.09 mm (caudal displacement) for the change from neutral to flexion posture, 8.4° and −2.5 mm (cranial displacement) for the change from neutral to extension posture, and 32.2° and 11.64 mm (caudal displacement) for the change from extension to flexion posture. The ΔLSA strongly correlated (ρ = 0.705; 95% CI, 0.434 to 0.859) with displacement of the CM.

CONCLUSIONS AND CLINICAL RELEVANCE
The use of dynamic MRI, compared with conventional MRI, will better help to characterize clinically normal and abnormal features of the lumbosacral region of the vertebral column and associated spinal cord during postural changes. Further, when limited translocation of the CM is evident on dynamic MRI, veterinarians should suspect underlying lumbosacral pathophysiologic processes or anatomic abnormalities.
Determination of Quality Characteristics, Phenolic Compounds and Antioxidant Activity of Propolis from Southeastern Mexico
Authors: Sauri-Duch Enrique , Gutiérrez-Canul Cesia , Cuevas-Glory Luis F. , Ramón-Canul Lorena , Pérez-Pacheco Emilio , Moo-Huchin Víctor M. 
Published: June 2021

Abstract
The objective of this work was to investigate the variability of physicochemical parameters, phenolic compounds and in vitro antioxidant activity of propolis collected from different apiaries in southeastern Mexico. A high variability was found in the moisture content (1.96–8.26%), ash (0.66–5.50%) and sensory characteristics of raw propolis from southeastern Mexico, but the raw propolis samples met the requirements of the quality regulations. In the same way, most of the ethanolic extracts also complied with the quality regulations. Of all the extracts, PE2 obtained from Santa Cruz showed the highest values for dry extract, content of total phenolic compounds (TPC), total flavonoids (TF) and antioxidant activity (DPPH and ABTS). The content of the individual phenolic compounds varied according to the geographical location of the apiary, but the PE2 extract resulted in the highest pinocembrin and chrysin content. A positive correlation was obtained between TPC and TF with antioxidant activity. Propolis extracts were classified into two groups through principal component analysis (PCA). These results indicate that the apiary location in southeastern Mexico influenced the characteristics of propolis.
Reakcje duchowieństwa w Trójmieście na zapowiedzi normalizacji stosunków między państwem a Kościołem rzymskokatolickim po Grudniu ’70
Authors: Abryszeński Piotr , Gucewicz Daniel 
Published: June 2021

Zmiany personalne na najwyższych stanowiskach państwowych wywołane brutalnym stłumieniem protestów w Grudniu ’70 spotkały się z pozytywną reakcją większości społeczeństwa, również duchownych Kościoła katolickiego. Jednak władze zdawały sobie sprawę, że nie wystarczy to do szybkiego uspokojenia nastrojów. Jednym z narzędzi służących legitymizacji nowej ekipy rządzącej stała się deklaracja gotowości do dialogu i normalizacji stosunków z Kościołem. Była ona sygnałem skierowanym zarówno do duchowieństwa, jak i do wiernych. Proces rozpoczęła wymiana korespondencji prymasa Stefana Wyszyńskiego z premierem Piotrem Jaroszewiczem, co otworzyło drogę do spotkań przedstawicieli Episkopatu Polski z przedstawicielami władz PRL. Rozmowy te przeniosły się również na poziom lokalny.
Deklaracje współpracy oraz towarzyszące im kurtuazyjne gesty zasadniczo spotykały się z zadowoleniem księży. Liczyli oni, że dzięki temu uda się pozytywnie zakończyć wiele nierozwiązanych dotychczas spraw i postulatów zgłaszanych władzom. Efektem rozmów było uregulowanie kwestii własności na Ziemiach Zachodnich i Północnych, uchylenie urzędowego obowiązku prowadzenia ksiąg inwentarzowych przez duchowieństwo czy realizacja części kościelnych postulatów dotyczących budownictwa sakralnego. Symbolem złagodzenia polityki władz była zgoda na budowę kościoła na gdańskim Przymorzu – dynamicznie rozwijającej się dzielnicy, której mieszkańcy od lat zabiegali o swoją świątynię. Znamienny był również zwrot kościoła św. Stanisława w Gdańsku‑Wrzeszczu na cele sakralne, o co bezskutecznie starano się od zakończenia wojny. Komuniści nie byli chętni do zbyt dużych ustępstw i stopniowo odzyskiwali kontrolę nad procesem normalizacji. Jednak nawet sam prymas Wyszyński dostrzegał pozytywne strony tego procesu.
Artykuł przedstawia pierwsze reakcje duchowieństwa w Trójmieście na normalizację stosunków między państwem a Kościołem rzymskokatolickim po rewolcie grudniowej 1970 r. Jest rozwinięciem i uzupełnieniem tez zawartych w książce Grudniowa kolęda. Kościół katolicki w Trójmieście wobec Grudnia ’70 (Gdańsk–Warszawa 2020).
Project-Based Versus Traditional Lecture Teaching Methods
Authors: Hafeez Muhammad 
Published: June 2021

To compete the world in 21st century, it is necessary to adopt the modern learning strategies like inquiry-based, project-based, problem-based. The traditional learning strategies are no viable now to improve the learning skills of the learners. A review of previous studies on traditional lecture-based and project-based learning strategies has been conducted to evaluate the effectiveness of both learning strategies. The learning outcomes and statistical results of 15 previous published articles have selected for this review study. The learning outcomes and statistical results of previous literature indicated that all the studies showed the significant improvement in the learning and cognitive skills of the learners. The effectiveness of project-based teaching strategy in various educational levels are also described in the study.
Gadolinium Orthovanadate GdVO4:Eu3+ Nanoparticles Ameliorate Carrageenan-Induced Intestinal Inflammation
Authors: Tkachenko Anton , Pogozhykh Denys , Onishchenko Anatolii , Myasoedov Valeriy , Podrigalo Leonid , Klochkov Vladimir , Chumachenko Tetyana , Prokopyuk Volodymyr , Yefimova Svetlana , Gubina-Vakulyck Galina , Kavok Nataliya , Butov Dmytro , Andrieiev Andrii , Polikarpova Hanna , Nakonechna Oksana 
Published: June 2021

Gadolinium orthovanadate GdVO4:Eu3+ nanoparticles (VNPs) have been shown to scavenge reactive oxygen species (ROS), making them a promising therapeutic agent in inflammation. This study aims to assess the effects of VNPs administered orally on E407a-induced inflammation. Materials and Methods: Fragments of the small intestine of 8 rats treated orally with a carrageenan-containing food additive E407a at a dose of 140 mg / kg of weight during 2 weeks, 8 animals orally exposed to both E407a and VNPs at a dose of 20 μg / kg of weight during the same period of time, and 8 control rats were stained routinely and immunostained for CD3 and CD68 with the subsequent immunohistochemical scoring. Moreover, analysis of viability and cell death modes of granulocytes was performed by flow cytometry using Annexin V and 7-aminoactinomycin D (7-AAD). Results: Oral exposure to the food additive E407a resulted in the development of enteritis associated with altered small intestinal morphology, infiltration of the lamina propria with macrophages and T-lymphocytes, and activation of peripheral blood granulocyte apoptosis. VNPs administered against the background of E407a-induced slight intestinal inflammation improved small intestinal morphology, decreased infiltration rate of the immune cells mentioned above without affecting the intensity of granulocyte apoptosis. Conclusion: Oral administration of VNPs ameliorates E407a-induced enteritis.
Efektivitas pengomposan sampah organik menggunakan electrical rotary aerobic composter (ERAC)
Authors: Iswanto Iswanto , Puspawati Catur , Suwerda Bambang 
Published: June 2021

Organic waste that produced in The Polytechnic Health Yogyakarta campus has not been processed and just disposed to Piyungan Landfill. To recycle the organic waste is required engineering technology that can produce compost quickly. Electrical Rotating Aerobic Composter (ERAC) is an aerobic powered electric composter model that is designed to rotate continuously for maintaining the stability of environmental factors (oxygen, temperature, humidity) and the homogeneity of processed organic matter. This study aims to determine effect and effectiveness of rotation speed of the ERAC on duration and quality of compost. This research is an experiment with Post-Test Design Only with Control Group Design. The sample of this research is organic waste that taken by proportionate stratified random sampling in The Polytechnic Health Yogyakarta campus. The composition of organic materials filled in the ERAC consist of 1 part organic waste from the canteen, 1 part of the yard / garden waste, 1 part of cattle manure and 5% active bioactivator. Rotation speed of the ERAC was set with variations: A = 0.20 rpm; B = 0.10 rpm; C = 0.05 rpm; K = 0.00 rpm (control). Each treatment was repeated 3 times and monitored daily until compost was formed. Compost samples are sent and checked for quality in the laboratory. Based on the physical parameters (temperature, color, texture and smell) of compost, showed that ERAC is able to process organic waste into compost within 7 days or four times faster than control (28 days). However, the variation of rotation speed was not significantly affect duration of composting. The quality of compost that produced by ERAC has fulfilled SNI No. 19-7030-2004 including C/N ratio, water content, N, P, K and C-organic. The compost quality according to rotation speed of the ERAC (0,20 rpm; 0,10 rpm; 0, 05 rpm, control (0.00 rpm)) respectively are: C / N ratio (19,83,19,84,19,95,19,90); water content (11,36%; 10,81%; 10,83%; 10,97%); N (1.00%; 1.06%; 1.11%; 1,024%); P (0,57%; 0,60%; 0,58%; 0,64%); K (1.37%; 1.32%; 1.30%; 1.28%); C-organic (19.69%; 20,99%; 21,90%; 20,18%).The result of statistical analysis with Kruskal Wallis test with SPPS program version 16 showed that water content, N, P, C-organic and C / N ratio between the three variations of rotation speed of ERAC is not significantly different, except K level. But when it was associated with workload of the ERAC engine, the lowest rotation speed (0.05 rpm) is the most effective.