bluuebunny/crossref_metadata_2025_split huggingface dataset:
PSXVI-3 Supplemental effects of Bacillus sp. in prevention of post-weaning diarrhea caused by enterotoxigenic F18+ Escherichia coli in nursery pigs
Authors: Duarte Marcos E , Kim Sung Woo 
Published: October 2021

Abstract
This study investigated the effects of Bacillus sp. on intestinal health and prevention of diarrhea by F18+ Escherichia coli (ETEC) in nursery pigs. Forty-eight weaned pigs (7.9 ± 0.5 kg BW) were allotted to 4 treatments with 12 replicates (NC: no-challenge; PC: challenge/no-treat; BMD: challenge/bacitracin; BAC: challenge/Bacillus sp. at 109 CFU/kg feed). All pigs were fed diets for 28 d. At d 7, challenged groups were orally inoculated with ETEC (1.8 × 1010 CFU) and NC group received sterile solution. Growth performance was analyzed weekly and pigs were euthanized on d 28 to measure intestinal health. Data were analyzed using SAS 9.4. There were no difference on growth performance among treatments during pre-challenge period. From d 7 to 14, PC tended to reduce (P = 0.067) ADG (352 to 440 g/d), whereas no effect was observed during the overall period. The PC increased (P &lt; 0.05) fecal score at d 7 (post-challenge) (4.1 to 3.3) and d 8 (4.1 to 3.3), whereas BMD decreased (P &lt; 0.05) it at d 9 (4.3 to 3.5) and d 11 (3.8 to 3.3). The PC increased (P &lt; 0.05) MDA (0.56 to 0.30 μmol/mg protein) and tended to increase TNFα (P = 0.084; 0.89 to 1.04 pg/mg protein), whereas BMD and BAC reduced (P &lt; 0.05) MDA (0.56 to 0.31, 0.29 μmol/mg protein) and TNFα (1.04 to 0.81, 0.77 pg/mg protein) in jejunal mucosa. The PC reduced (P &lt; 0.05) α-diversity (Chao1: 32.3 to 19.4), whereas BAC increased it (Chao1: 19.4 to 30.7) whereas BMD increased (P &lt; 0.05) it (Shannon: 1.85 to 2.44; Simpson: 0.55 to 0.70) of jejunal mucosa-associated microbiota. In conclusion, ETEC challenge caused diarrhea, increased immune response, and oxidative stress by disrupting mucosa-associated microbiota. The BMD and BAC reduced jejunal immune response and oxidative stress by restoring mucosa-associated microbiota.
PSXVI-4 Supplemental effects of increasing levels of soy protein concentrate replacing animal protein supplements on growth performance and intestinal health of nursery pigs
Authors: Deng Zixiao , Duarte Marcos E , Kim Sung Woo 
Published: October 2021

Abstract
This study evaluated supplemental effects of increasing levels of soy protein concentrate (SPC) replacing animal protein supplements on growth performance and intestinal health of nursery pigs. Thirty-two newly weaned pigs (6.4 ± 0.4 kg BW) were allotted to 4 treatments in a RCBD with initial BW and sex as blocks and fed for 35 d in 3 phases (P1/2/3 for 10/12/13 d, respectively) following NRC (2012). Dietary treatments were SPC-0 (diet with fish meal 4/2/1%, poultry meal 10/8/4%, and blood plasma 4/2/1% for P1/2/3, respectively); SPC-1, SPC-2, and SPC-3 (SPC-0 with SPC replacing 1/3, 2/3, and 3/3 of animal protein supplements). Titanium dioxide (0.4%) was added to the diets as indigestible marker from d 30. Growth performance was recorded for each phase. Pigs were euthanized on d 35 to collect jejunal tissue to measure intestinal health, and ileal digesta to measure apparent ileal digestibility (AID) of nutrients. Data were analyzed using the MIXED procedure of SAS. Overall, increasing levels of SPC replacing animal proteins reduced linearly (P &lt; 0.05) the BW (21.4 to 17.3 kg), ADG (431 to 312 g/d), and ADFI (551 to 390 g/d) of pigs. The G:F decreased linearly (P &lt; 0.05) on P1 (0.72 to 0.52). Increasing levels of SPC replacing animal proteins did not affect the AID of nutrients. Immunoglobulin A tended to increase (P = 0.099, 3.39 to 5.55 mg/mg of protein) when SPC supplemental levels were compared with the control group. Increasing levels of SPC replacing animal proteins did not affect the concentration of MDA, TNF-a, IL-8, protein carbonyl, and IgG and villus height, villus width, and crypt cells proliferation among treatments. Collectively, increasing level of SPC supplement did not negatively affect the intestinal health and digestibility of nutrients, whereas linearly reduced the growth performance.
PSXVI-7 Supplemental effects of Lactobacillus extract postbiotics in prevention of post-weaning diarrhea caused by enterotoxigenic F18+ Escherichia coli in nursery pigs
Authors: Xu Xiangyi , Duarte Marcos E , Kim Sung Woo 
Published: October 2021

Abstract
This study determined the supplemental effects of Lactobacillus extract (LBE) postbiotics on intestinal health and prevention of postweaning diarrhea caused by F18+ Escherichia coli (ETEC) in nursery pigs. Sixty-four weaned pigs (6.6 ± 0.7 kg BW) were allotted in a RCBD to 4 dietary treatments (NC: no-challenge; PC: challenge/no-treat; BMD: challenge/bacitracin; LBE: challenge/LBE 0.2%) and fed diets for 28 d. At d 7, challenged groups were orally inoculated with ETEC (2.4 x 1010 CFU) and NC group received sterile solution. Growth performance was analyzed weekly and pigs were euthanized on d 28 to measure intestinal health. Data were analyzed using the SAS 9.4. During post-challenge period, PC tended to decrease (P = 0.067) ADG (373 to 284 g/d), whereas BMD increased (P &lt; 0.05) ADG (284 to 408 g/d); LBE tended to increase (P = 0.081) ADG (284 to 370 g/d). PC increased fecal score (P &lt; 0.05, 3.4 to 3.9) on d 14, whereas BMD decreased it (P &lt; 0.05, 3.9 to 3.5) on d 21. PC increased (P &lt; 0.05) protein carbonyl (0.76 to 1.12 nmol/mg protein), crypt cell proliferation (28 to 36%), and Helicobacter rodentium (0.4 to 3.7%). However, BMD decreased (P &lt; 0.05) crypt cell proliferation (36 to 32%) and Helicobacter spp. (15.0 to 1.4%); and increased (P &lt; 0.05) villus height (309 to 377 µm), Bifidobacterium boum (0.04 to 2.0%), Pelomonas spp. (1.5 to 8.5%), and Microbacterium ginsengisoli (0.5 to 3.0%). LBE reduced (P &lt; 0.05) crypt cell proliferation (36 to 27%) and Helicobacter rodentium (3.7 to 0.04%); and increased (P &lt; 0.05) Lactobacillus salivarius (0.3 to 4.1%) and Propionibacterium acnes (0.4 to 7.4%). Collectively, ETEC reduced growth performance by adversely affecting microbiota and intestinal health. BMD and LBE improved growth performance by enhancing intestinal health and increasing beneficial microbiota in ETEC challenged pigs.
PSXII-22 Relationship between beef cow fertility and maternal immunity
Authors: Ahmadzadeh Amin , Corpron McKenzie , Rezamand Pedram , Huo Qun 
Published: October 2021

Abstract
Immunity plays important role in reproduction. There is evidence that maternal immunity changes from a type 1 to a type 2-biased during pregnancy and lack of such a shift may contribute to unsuccessful pregnancy in animals. The objective of the study was to examine the relationship between relative balance between type 1 and type 2 and fertility in suckled beef cows by characterizing relative quantity ratio of IgG 1 and 2 subclasses (IgG1/IgG2). Angus-Hereford cows (n = 394) were synchronized (d0) with a 5-d CIDR protocol. Estrous behavior was monitored using estrus detection aids and visual observation from d5 until AI (d8). On d8 all cows were inseminated at a fixed time and blood samples were collected form to be examined for type1/type/2 ratio and progesterone (P4). A new rapid blood test (D2Dx) was used to assess the type 1/type 2 immunity balance by measuring the relative quantity ratio of IgG1 and IgG2. Pregnancy was determined by ultrasound 48 to 63 days after AI. Overall pregnancy per AI was 51%. Mean relative quantity of IgG1/IgG2 was different (P &lt; 0.05) between non-pregnant and pregnant cows. There was negative relationship between relative IgG/IgG2 ratio and probability of pregnancy to AI (P &lt; 0.05). As IgG1/IgG2 ratio increased, probability of P/AI decreased. Mean relative IgG1/IgG2 ratio was lesser (P &lt; 0.05) in cows detected in estrus vs non-estural cows. There was no correlation between serum P4 concentrations and IgG1/IgG2 ratio. The results indicate that blood IgG1/IgG2 at the time AI has a potential to be used as a marker for pregnancy prediction in beef cows.
PSXII-16 Fertility affects in yearling beef heifers grazing triticale before artificial insemination
Authors: Johnson Sandy , Holman John 
Published: October 2021

Abstract
Our objective was to compare two systems of pre-breeding heifer development, drylot (D) or grazing winter triticale (T) on heifer fertility. In each of 4 years (yr), Black Angus heifers (n = 168, 86, 97 and 103, respectively) were allotted to treatment (trt) 32 to 50 days (d) prior to artificial insemination (AI). Each year a 14-d CIDR PG protocol was used to synchronize estrus (d-30 to d-16: 1.38 g progesterone intravaginal insert, CIDR; d 0: 25 mg dinoprost tromethamine injection; + 66 hours: 100 µg gonadorelin hydrochloride injection, and AI). Starting body weight and body condition were similar between treatments but differed by year (P &lt; 0.01). After CIDR removal, 83% were in estrus by d -10 and did not differ with trt or yr. Weight at AI was greater (P &lt; 0.01) for T (384 kg ± 2) than D (378 kg ± 2) with a trt by yr interaction. Body condition change between start of treatment and AI was greater (P &lt; 0.01) for T (0.44 ± 0.03) than D (-0.02 ± 0.04) with a trt by yr interaction (P &lt; 0.01) due to the magnitude of difference. A trt by yr interaction occurred for estrous response before AI (P &lt; 0.01). Treatment means for AIPR were 59.1% (143/242) for D and 42.1% (101/240) for T (trt; P &lt; 0.01). AIPR was similar between treatments in yr 1 and higher for D than T in subsequent yr (trt x yr, P &lt; 0.05). Blood urea nitrogen was greater (P &lt; 0.05) in T than D on d 0 and at AI. At the time of AI, triticale was more mature in yr 1 when there was no difference in AIPR. Grazing triticale during estrus synchronization and AI resulted in acceptable gains but decreased fertility in yearling beef heifers.
PSXII-14 Estimation of phosphatidylserine positive sperm in fresh bull semen
Authors: Haines Hannah , Stewart Jamie L , Wege Dias Nicholas W , Pancini Stefania , Clark Sherrie , Leeth Caroline , Lysiak Jeffrey , Rodrigues Gomes Mercadante Vitor R G 
Published: October 2021

Abstract
Previous research demonstrated that phosphatidylserine (PS), a marker for apoptosis, plays a role in murine sperm fertilization capacity; however, less is known of the role of PS in bovine fertilization. The objective of this experiment was to determine the concentration of PS positive sperm on fresh bovine semen (n = 4). A breeding soundness evaluation was performed and electroejaculation was used to collect semen which was immediately evaluated for ejaculate density and gross motility through visual evaluation. Semen smears of semen mixed with an eosin-nigrosin strain were prepared on glass microscope slides. Semen was mixed with OptiXcell™ (IMV Technologies) semen extender, a 0.5 mL sample from each bull was washed with non-capacitating media and then incubated at 37.5°C 5% CO2 with capacitating media for 60 minutes. Samples were stained with Annexin V FITC and 7AAD for 15 minutes each. Samples were analyzed using a Flow Cytometer to determine the percentage of PS positive sperm. Bull 1 had a scrotal circumference of 37.5 cm, ejaculate density of 400–750 million sperm/mL, very good gross motility, 78% sperm with normal morphology, and 14.65% PS positive sperm. Bull 2 had a scrotal circumference of 37 cm, ejaculate density of 400–750 million sperm/mL, very good gross motility, 84% sperm with normal morphology, and 17.05% PS positive sperm. Bull 3 had a scrotal circumference of 39.5 cm, ejaculate density of 250–400 million sperm/mL, fair gross motility, 48% sperm with normal morphology, and 12.8% PS positive sperm. Bull 4 had a scrotal circumference of 38 cm, ejaculate density of 400–750 million sperm/mL, good gross motility, 68% of sperm with normal morphology, and 12.65% PS positive sperm. Our data demonstrate the possibility of identifying live PS positive sperm in fresh ejaculate from bulls. The possibility exists that PS plays a role in sperm oocyte fusion and fertilization in the bovine.
PSXII-13 Blood flow parameters in ovine fetuses from different sires
Authors: Checura Celina M , Atkinson Katherine , Boulos Natalie , Crowfoot Emma , Friend Hannah , Jackson Sophia , Lubowicki Shelby , Silva Camila , Bridges William , Duckett Susan K , Walker Eric 
Published: October 2021

Abstract
Placental and fetal development are influenced by paternal genes. Doppler ultrasonography was performed on pregnant ewes to examine the effect of sire on fetal and placental blood flow parameters. Twenty Suffolk ewes were randomly assigned to either a Suffolk ram or a Texel ram. Only ewes carrying twins were used for the study. Ultrasound exams were performed at 60, 90, and 120 (± 3) days post-breeding. Umbilical artery and fetal aorta blood-flow velocities, and aorta vessel diameter were measured. Blood flow velocities were used to calculate the resistance index (RI), which was used for statistical analysis (Glimmix, SAS). Gestational age and weight at birth were recorded. There were 5 Suffolk x Suffolk (SxS) and 6 Suffolk x Texel (SxT) ewes carrying twins to term. For umbilical artery RI, there was no significant (P &lt; 0.05) effect of breed; there was significant effect of time (0.89 ± 0.03a; 0.63 ± 0.03b; 0.59 ± 0.03b LSM ± SE for days 60, 90, and 120 respectively); and there was no interaction. For aorta RI, there was no significant effect of breed; there was significant effect of time (0.73 ± 0.03a; 0.67 ± 0.03ab; 0.63 ± 0.03b for days 60, 90, and 120 respectively); and there was no interaction. For aorta diameter, the effect of breed approached significance (P = 0.054), SxS had a larger average diameter (0.50 ± 0.02 cm) than SxT (0.45 ± 0.01 cm); there was significant effect of time (0.24 ± 0.03a; 0.46 ± 0.02b; 0.72 ± 0.02c cm for days 60; 90; and 120 respectively); and there was no interaction. There were no significant differences between sires for weight or gestational age at birth. The results correspond with the changes in fetal and placental vascular resistance over time; however, further research is needed to expand our knowledge on the effect of sire.
PSXII-17 Fertility prediction model for Nilli-Ravi buffalo bulls through Fourier harmonic analysis of sperm
Authors: Parrish John J , Arshad Javeria , Awan M A , Akhter S 
Published: October 2021

Abstract
A model to predict Nili-ravi buffalo bull fertility was developed based on Fourier harmonic analysis of sperm. Seventeen bulls with 3032 AI records were categorizes based on fertility rate (FR) as low (36.5±0.2, n = 6: SD&lt; ˗1 from mean FR), medium (39.9±0.2, n = 3; SD +1 to -1 from mean FR) and high fertility (41.4±0.1, n = 8; SD &gt; +1 from mean FR). Cryopreserved semen samples from these bulls were investigated for Fourier harmonic analysis of sperm nuclear shape. Hoechst-33342 and YOYO-1 fluorescent stains were used to identify live and dead sperm. Digital images were analyzed to get sperm nuclear perimeter points at different phase angles to generate Fourier functions. Mean harmonic amplitude (HA) 0 was different (P &lt; 0.05) for 1700 live vs. 1294 dead sperm from the 17 bulls, thus live sperm were used for remaining analyses. The mean, variance, skewness and kurtosis values of 100 live sperm nuclei/bull were compared for HA0-5 between high (n = 6) and low (n = 6) fertility groups, considering equal number of bulls in each category. The mean HA2 (0.739±0.01 vs 0.686±0.00) and 4 (0.105±0.001 vs 0.007±0.001) were higher in high vs low fertility group respectively (P &lt; 0.05). Sperm nuclear perimeter among high fertility group sperm was more elongated. There was also an increased skewness of HA0 as fertility increased (P &lt; 0.05). Discriminant analysis defined a fertility model by using mean HA4, skewness HA0 and variance HA2, that resulted in 91.7% bulls into their correct fertility group upon cross-validation (canonical correlation=0.928; P &lt; 0.05). Higher values of mean HA4, skewness HA0 and variance HA2 increase the chance of bulls being placed in the high fertility group. In conclusion, sperm nuclear shape in Nili-ravi buffalo bull is related to in vivo fertility. A fertility model using reported discriminant measures could be used to objectively identify Nili-ravi buffalo bulls of varying fertility.
PSVIII-18 Effect of rumen temperature on the average daily gain of growing-finishing calf-fed Holstein steers in the feedlot
Authors: Carvalho Pedro H , Latack Brooke C , Zinn Richard A 
Published: October 2021

Abstract
Objectives were to evaluate the relationship between ruminal temperature and average daily gain of calf-fed Holstein steers during the extreme summer months characteristic of the Southern California desert region. Thirty-eight calf-fed Holstein steers (395 ±25 kg) were utilized in the 84-d experiment (June 30 – September 20, 2020). Steers were blocked by initial shrunk weight (168 d before the initiation of the experiment) and randomly assigned to 38 pens with 4 other steers (5 steers per pen). Pens were 62 m2 with 25 m2 overhead shade, automatic waterers and 2.4 m fence-line feed bunks. All steers were fed a steam-flaked corn-based growing-finishing diet. A bolus measuring temperature and activity at ten-minute intervals (smaXtec Basic, SmaXtec Animal Care GmbH, Austria) was placed in the rumen of each steer 112 d before the initiation of the experiment (March 10, 2020). Air temperature for the trial averaged 34.90 ± 3.03°C (maximum = 43.8 ± 2.97° C; minimum 24.72 ± 4.00°C). Average temperature-humidity-index (THI) was 80.1 ±5.4. Maximum THI during the 84-d period averaged 89.29 ± 7.85 (steers experience “emergency” THI category for all 84 days of the trial). Maximum (RTmax) and mean (RTm) daily ruminal temperature averaged 40.93 ±0.24 and 40.21 ±0.23°C, respectively. Average daily gain (mean = 1.18 kg/d; maximum = 1.54 kg/d; minimum = 0.88 kg/d) increased (P &lt; 0.01) as RTm decreased (ADG, kg = 19.13 – 0.45 RTm; r2 = 0.28). There was a close association between RTmax and RTm (RTm = 4.57 + 0.87 RTmax; r2 = 0.84). While there are numerous factors that influence ADG of feedlot cattle, differences in ruminal temperature during periods of elevated ambient conditions (ie. temperature, humidity, ration) accounts for an important portion of that variation.
PSVIII-14 Effect of Certillus, a direct-fed microbial, on milk yield, milk components, and feed intake in primi- and multiparous lactating Holstein cows
Authors: Maylem Excel Rio S , Vargiu Martina , Melton Caleb , Thompson Jesse , O’Neill John , Rehberger Tom G , Foote Andrew P , Spicer Leon J 
Published: October 2021

Abstract
To determine the effect of Certillus on milk production, milk components and feed efficiency, primiparous (n = 13) and multiparous (n = 12) Holstein cows were assigned to one of two dietary groups starting from 8 to 120 days of lactation. The controls (n = 13) received regular TMR ration, and treated cows (n =12) received control TMR plus 2 x 109 cfu/head of Certillus (Church & Dwight Co., Inc., Waukesha, WI). Cows were housed in an open-air free-stall barn with 16 Insentec electronic feeders (8 feeders/group) to record daily feed events and intake (DMI). Cows were provided with ad libitum TMR fed twice daily and were milked twice daily. Milk samples were taken weekly during successive a.m./p.m. milkings and analyzed for percentage milk fat, protein, lactose, and milk urea nitrogen (MUN). Data were analyzed using a repeated-measures 2 x 2 factorial ANOVA. Daily milk production (4%FCM) was influenced (P &lt; 0.05) by treatment x parity, week and parity, averaging 11% greater (P &lt; 0.05) in Certillus-fed than control cows. Milk fat percentage increased (P &lt; 0.005) from 4.02 to 4.41 + 0.09% with Certillus feeding. Milk lactose was influenced (P &lt; 0.05) by week and treatment x parity. Milk protein was affected by week (P &lt; 0.001) only. Certillus decreased (P &lt; 0.05) MUN levels such that MUN was 1.0 mg/dL greater (P &lt; 0.001) in Certillus-fed than control cows. Feed intake was reduced by 9% (P &lt; 0.01) and feed efficiency (kg 4%FCM/kg DMI) was improved by 14% (P &lt; 0.05) in Certillus-fed versus control cows. We conclude that Certillus may hold potential as an effective direct-fed microbial to increase feed efficiency and milk production.
PSVIII-17 Effect of high-tannin sorghum grain on in vitro rumen fermentation and methane production
Authors: Molho-Ortiz Aarón Alejandro A , Corona Luis , Márquez-Mota Claudia Cecilia , Beauchemin Karen A , Talamantes-Gómez José Moisés , Ramírez-Bribiesca Efrén , Romero-Pérez Atmir 
Published: October 2021

Abstract
The objective was to evaluate the potential of methane (CH4) mitigation of high-tannin sorghum (HTS) varieties (V). The experiment was arranged as a completely randomized block design with a 7 × 2 factorial arrangement of treatments, including 6 HTS varieties (BRS72, Dekalb; 81G67, Dupont-Pioneer; QL-BR95, Lucava; 6001, ACA; NYX, Unisem; Red Bird, Warner Seeds; 20.1±3.2 g/kg condensed tannins), a low tannin sorghum (LTS, 0.3 g/kg condensed tannins) and two levels of polyethylene-glycol (PEG; 0 and 1 g). Three independent batch culture runs were conducted using a basal diet (0.5 g) containing 72% sorghum, 18% corn stover and 10% soybean meal. Total gas production (TGP), CH4 production, gas production kinetics and dry matter digestibility (DMD) were evaluated. Data were analyzed with R studio 1.4.1106 using a mixed model and the Tukey instruction for mean comparison. Interaction (V × PEG) was not significant (P = 0.16) for DMD. However, DMD decreased (P &gt; 0.05) 6.1 percentage units with HTS varieties compared to LTS. Furthermore, PEG increased (P &gt; 0.05) DMD by 1.78 percentage units. The V × PEG interaction tended to be significant for TGP (P = 0.09; mL/g DM), where sorghum 81G67 + PEG showed the lowest gas production (486 mL/g DM). Kinetics of gas production were also affected. Fermentation rate was higher (P &gt; 0 .05) for the LTS variety (0.0373 %/h) and lower (P &gt; 0.05) for sorghum 81G67 (0.0317 %/h), while maximum volume of gas was 5.9% higher (P &gt; 0.05) when PEG was included. Methane concentration (%) was lowest (P &lt; 0.05) with QL-BR95 sorghum but CH4 production (mL/g DM) was not affected (P = 0.38) by sorghum variety. Methane concentration and production increased (P &gt; 0.05) 8.9 and 4.1% respectively with PEG inclusion. In conclusion, HTS negatively affected digestibility and rumen fermentation while PEG minimized such effects. Sorghum grain varieties with high tannin content had negligible effects on rumen methanogenesis.