bluuebunny/crossref_metadata_2025_split huggingface dataset:
Prediction of Deleterious nsSNPs Causing CHARGE Syndrome Associated with the CHD7 Protein using Computational Approaches
Authors: Rathinam Nithya S. , N Madhana Priya , Ramasamy Magesh 
Published: February 2021

Abstract
The Chromo domain helicase DNA binding protein 7 (CHD7) is also known as ATP-dependent helicase CHD7, in humans, the CHD7 gene encodes it. Heterozygous mutations in this protein cause aggregation and has been determined to have an adverse role in causing CHARGE syndrome. Non-synonymous single nucleotide polymorphism (nsSNP) analysis tends to be deleterious of the protein yet to be employed with computational methods though being the highlight for novel investigations. Various computational methods were used to categorize the 201 identified nsSNPs in the catalytic domain of the CHD7 protein (the nsSNPs are observed to have a damaging effect in the catalytic domain). Pathogenicity analysis determined 81 nsSNPs to be pathogenic and further narrowed down to 61 nsSNPs by stability analysis. Based on the structure availability, the two nsSNPs (P2683S and R2702C) were selected and were checked in the computational tools for sequence analysis (pathogenicity analysis, stability analysis, physiochemical property analysis, and conservational analysis) and were determined to have a high impact over the protein molecule. The molecular dynamics simulation and principal component analysis (PCA) were performed to determine the conformational stability and flexibility change of the proteins. Subsequently, a molecular dynamic simulation (MDS) for 100ns was performed to understand the impact of the differences between the native and the mutant structures of the CHD7 protein. The simulation plots disclose very minute changes in patterns of stability, residue fluctuation, structure compactness, and flexibility regarding P2683S and R2702C mutation compared to the native structure. Further, Molecular docking was performed for the native and the mutant structures P2683S and R2702C to study the binding efficacy of the drugs Methyltestosterone and Estradiol resulting in a similar score with a very little difference to each other. The Native and mutants P2683S and R2702C have similar interaction of -5.7 kcal/mol, -5.9 kcal/mol and − 5.6 kcal/mol respectively with Methyltestosterone followed by a binding score of -6 kcal/mol, -5.6 kcal/mol and − 5.8 kcal/mol respectively for Estradiol. Detailed study about the disease, effect of nsSNP’s and the response of the drug towards the mutation are the key factors in order to launch a new personalized medicine. Therefore, in this study using various computational prediction methods, molecular dynamics simulation and molecular docking studies we have determined the nsSNP’s responsible to cause CHARGE syndrome and the drug response with respect to the determined nsSNP mutations. The outcomes acquired from our investigation will provide the data for experimental biologists for the additional procedure for examining the rest of the variations in CDH7 protein.
The Optimal Time of Applying Enteral Immunonutrition in Esophageal Cancer Patients Receiving Esophagectomy: A Network Meta-Analysis of Randomized Clinical Trials
Authors: Tian Xu , Jin Yan-Fei , Zhang Zhao-Li , Chen Hui , Chen Wei-Qing , Jiménez-Herrera Maria F. , Han Yang 
Published: February 2021

Abstract
Background: Enteral immunonutrition (EIN) has been extensively applied in cancer patients, however its role in esophageal cancer (EC) patients receiving esophagectomy remains unclear. We performed this network meta-analysis to investigate the impact of EIN on patients undergoing surgery for EC and further determine the optimal time of applying EIN.Methods: We searched PubMed, EMBASE, Cochrane library, and China National Knowledgement Infrastructure (CNKI) to identify eligible studies. Categorical data was expressed as the odds ratio with 95% confidence interval (CI), and continuous data was expressed as mean difference (MD) with 95% CI. Pair-wise and network meta-analysis was performed to evaluate the impact of EIN on clinical outcomes using RevMan 5.3 and ADDIS V.1.16.8 softwares. The surface under the cumulative ranking curve (SUCRA) was calculated to rank all nutritional regimes.Results: Total 14 studies involving 1071 patients were included. Pair-wise meta-analysis indicated no difference between EIN regardless of the application time and standard EN (SEN), however subgroup analyses found that postoperative EIN was associated with decreased incidence of total infectious complications (OR=0.47; 95%CI=0.26 to 0.84; p=0.01) and pneumonia (OR=0.47; 95%CI=0.25 to 0.90; p=0.02) and shortened LOH (MD=-1.01; 95%CI=-1.44 to -0.57; p<0.001) compared to SEN, which were all supported by network meta-analyses. Ranking probability analysis further indicated that postoperative EIN has the highest probability of being the optimal option in terms of these three outcomes.Conclusions: Postoperative EIN should be preferentially utilized in EC patients undergoing esophagectomy because it has optimal potential of decreasing the risk of total infectious complications and pneumonia and shortening LOH.OSF registration number: 10.17605/OSF.IO/KJ9UY.
Biocementation Mediated By Stimulated Ureolytic Microbes From Brahmaputra Riverbank For Mitigation of Soil Erosion
Authors: Dubey Anant Aishwarya , Ravi K , Mukherjee Abhijit , Sahoo Lingaraj , Akindele Abiala Moses , Dhami Navdeep Kaur 
Published: February 2021

Abstract
Riverbank erosion is a global problem with significant socio-economic impacts. Microbially induced carbonate precipitation (MICP) has recently emerged as a promising technology for improving the mechanical properties of soils. The presented study investigates the potential of native calcifying bacterial communities of the Brahmaputra riverbank for the first time via biostimulation and explores its effect on the mitigation of soil erosion. The ureolytic and calcium carbonate cementation ability of the enriched cultures were investigated with reference to the standard calcifying culture of Sporosarcina pasteurii (ATCC 11859). 16S rRNA analysis revealed Firmicutes to be the most predominant calcifying class with Sporosarcina pasteurii and Pseudogracilibacillus auburnensis as the prevalent strains. The morphological and mineralogical characterization of carbonate crystals confirmed the calcite precipitation potential of these communities. The erosion resistance of soil treated with native calcifying communities was examined via needle penetration and lab-scale flume erosion test. We found a substantial reduction in soil erosion in the biocemented sample with calcite content of 7.3% and needle penetration index of 16 N/mm. We report cementation potential of biostimulated ureolytic cultures for a cost-competitive and environmentally-conscious alternative to current erosion mitigation practices.
Postoperative transverse sternal nonunion with a chest wall defect managed by a tibial locking plate and a Gore-Tex dual mesh membrane: a case report.
Authors: Malovrh Tomaz , Stupnik Tomaz , Podobnik Boris , Kalisnik Jurij Matija 
Published: February 2021

Abstract
Background: Transverse sternal nonunion is a rare but disabling complication of the chest trauma or less commonly a transverse sternotomy. Fixation methods, which are mainly used to manage the more common longitudinal sternal nonunion, often fail leaving surgical treatment of transverse nonunion as a challenge.Case presentation: We present a case of a highly disabling postoperative chest wall defect resulting from transverse sternal nonunion after a transverse thoracosternotomy (clamshell incision) and a concomitant rib resection. Following unsuccessful surgical attempts, sternal nonunion was fixed by a tibial locking plate and bone grafted, while the chest defect after the rib resection was reconstructed by a Gore-Tex dual mesh membrane. Adequate chest stability was achieved enabling complete healing of the sternal nonunion and a good outcome in our patient.Conclusion: We believe that in a rare combined postoperative transverse sternal nonunion and the chest wall defect after rib resection, it is important to address both. In our patient a good outcome was achieved after fixing nonunion by a properly sized and shaped locking plate with bone grafting and covering the chest defect by a dual mesh membrane.
Development of Rotational Intraperitoneal Pressurized Aerosol Chemotherapy to Enhance Drug Delivery into the Peritoneum
Authors: Park Soo Jin , Lee Eun Ji , Lee Hee Su , Kim Junsik , Park Sunwoo , Ham Jiyeon , Mun Jaehee , Paik Haerin , Lim Hyunji , Seol Aeran , Yim Ga Won , Shim Seung-Hyuk , Kang Beong-Cheol , Chang Suk Joon , Lim Whasun , Song Gwonhwa , Kim Jae Weon , Lee Nara , Lee Jung Chan , Kim Hee Seung 
Published: February 2021

Abstract
Background
Pressurized intraperitoneal aerosol chemotherapy (PIPAC) has been introduced as palliative therapy for treating peritoneal metastasis (PM) of solid tumors. However, restricted use in the limited countries and the uneven distribution and penetration in various regions of the peritoneal cavity ac as disadvantages of PIPAC. Thus, the KOrean Rotational Intraperitoneal pressurized Aerosol chemotherapy (KORIA) trial group developed rotational intraperitoneal pressurized aerosol chemotherapy (RIPAC) for enhancing drug delivery into the peritoneum to treat PM, and evaluated the drug distribution, tissue concentrations, penetration depth, pharmacokinetic properties, and toxicities after RIPAC with doxorubicin in pigs.
Methods
For delivering doxorubicin as aerosols, we used our prototype for PIPAC, which sprayed about 30-µm sized droplets through the nozzle. The mean diameter of the sprayed region was 18.5 cm, and the penetration depth ranged from 360 to 520 µm, comparable to the microinjection pump (Capnopen®; Capnomed, Villingendorf, Germany). For RIPAC, a conical pendulum motion device was added to PIPAC for rotating the nozzle. RIPAC and PIPAC were conducted using 150 ml of 1% methylene blue to evaluate drug distribution and 3.5 mg of doxorubicin in 50 ml of 0.9% NaCl to evaluate tissue concentration and penetration depth, pharmacokinetic properties, and toxicities. All agents were sprayed as aerosols via the nozzle with a velocity of 5 km/h at a flow rate of 30 ml/min under a pressure of 7 bars, and capnoperitoneum of 12 mmHg was maintained for 30 minutes. As a control, we conducted early postoperative intraperitoneal chemotherapy (EPIC) using 1% methylene blue solution with an infusion flow rate of 100 ml/min for 30 minutes and the drainage of 1 L every 10 minutes.
Results
RIPAC showed a wider distribution and stronger intensity than EPIC and PIPAC. Moreover, the tissue concentration and penetration depth of doxorubicin were higher in RIPAC than in PIPAC. In RIPAC, the pharmacokinetic properties reflected hemodynamic changes during capnoperitoneum, and there were no renal and hepatic toxicities related to RIPAC using doxorubicin.
Conclusions
RIPAC may have the potential to enhance drug delivery into the peritoneum compared to PIPAC.
Long non-coding RNA HCG18 promotes malignant phenotypes of breast cancer (BC) cells by HCG18/miR-103a-3p/UBE2O/mTORC1/HIF-1α positive feedback loop
Authors: Liu Xu , Qiao Kun , Zhu Kaiyuan , Li Xianglan , Zhao Chunbo , Li Jiaqi , Feng Dawei , Fang Yu , Wang Peng , Qian Cheng , Qiao Wenbo 
Published: February 2021

Abstract
Background: In recent years, a growing number of studies have reported that long non-coding RNAs (LncRNAs) play crucial roles in breast cancer (BC) progression and metastasis. Another study group of our research center reported that LncRNA HCG18 was one of the 30 upregulated lncRNAs in BC tissues related to normal tissues in TCGA database. However, the exactly biological roles of HCG18 in BC remains unclear. Method: qRT-PCR was used to detect the expression profile of HCG18 in BC tissues and cell lines. In vitro assays were used to evaluate the pro-tumor function of HCG18 in BC cells. Animal study were used to explore the role of HCG18 in vivo. Bioinformatic analysis, dual-luciferase reporter assay, RNA immunoprecipitation (RIP) assay and Chromatin Immunoprecipitation (ChIP) assays were used to investigate the regulatory relationship of HCG18, miR-103a-3p, UBE2O in BC. Results: HCG18 was upregulated in BC tissues and cells, and BC patients with high HCG18 expression tended to have poor prognosis. HCG18 could promote BC cells proliferation, invasion and provided BC cells with tumor stemness properties (CSPs) in vitro and facilitate tumor growth and lung metastasis in vivo. In terms of mechanism, HCG18 functioned as a miRNA sponge which positively regulated the expression of Ubiquitin-conjugating enzyme E2O (UBE2O) by sponging miR-103a-3p and our previous research achievement have already verified UBE2O could promote malignant phenotypes of BC cells through UBE2O/AMPKα2/mTORC1 axis. Furthermore, as a downstream target of HCG18/miR-103a-3p/UBE2O/mTORC1 axis, HIF-1α transcriptionally promoted HCG18 expression and then formed a positive feedback loop in BC. Conclusion: HCG18 played an oncogenic role in BC and it might serve as a prognostic biomarker and a potential therapeutic target for BC treatment.
Facile Synthesis of Hollow Flower-Like SnO and Applications in Lithium Ion Batteries
Authors: Guo Yuxi , Xu Panpan , Xu Manzhang , Deng Ken , Zhang Zhiyong , Wang Yingnan , Zhao Wu 
Published: February 2021

Abstract
Novel hollow flower-like SnO structures have been successfully synthesized by using a facile solvothermal method approach, which injecting LiN(SiMe3)2 to the solution of SnCl2 and oleamine. The as-synthesized hollow flower-like SnO structures were aggregated by ultrathin SnO nanosheets with large surface area and exhibit narrow size distribution of ~2.0 µm. The electrochemical performance of hollow flower-like SnO structures were examined as an anode material in coin cells for lithium-ion batteries. It demonstrates excellent lithium ion batteries performance as characterized by the cycling stability, specific capacity, cyclic voltammetry and rate performance. The measured discharge capacity is 361.4 mAh g-1 after 50 charge/discharge cycles with 46% capacity retention, which indicated a improving in cyclic stability. This result demonstrated that the specific hollow structure has great potential as the electrodes for lithium ion batteries. This work may provide an attractive road to synthesize other hollow-flower structures of transition metal oxides.
Assessment of Hip Involvement in Patients With Ankylosing Spondylitis: The Reliability Between the HIMRISS and BASRI-Hip System
Authors: Man Siliang , Zhang Liang , Bian Tao , Li Hongchao , Ma Zhuyi , Zhou Yixin 
Published: February 2021

Abstract
Background. The aim is to test the reliability and validity of the Hip Inflammation MRI Scoring System (HIMRISS) in assessing hip involvement of AS patients at different stages of the bath ankylosing spondylitis radiology index (BASRI-hip) scoring system.Methods. Fifty-two outpatients with ankylosing spondylitis(AS) were included in this study. The subjects’ data includes demographics, clinical characteristics, disease activity score, and functional index. Based on the Harris hip scoring (HHS) of involved hip and BASRI-hip score, we devided these patients into no hip involvement group(HHS≥80 and BASRI≤1)(Group A), mild hip involvement subgroup (BASRI=2 or BASRI≤1 and HHS≤79) (Group B) and moderate to advanced hip involvement subgroup (BASRI≥3) (Group C). Data was analyzed statistically by SPSS software.Results. In total of 44 patients (88 hips), group A consisted of 21 hips, group B consisted of 42 hips and group C consisted of 25 hips. The test-retest intraclass correlation coefficients (ICCs) in four raters were 0.955~0.977 and interrater ICC was 0.993. HIMRISS correlated moderately with the Bath Ankylosing Spondylitis Disease Activity Index (BASDAI) (r=0.540, p<0.001), the Bath ankylosing spondylitis functional index (BASFI) (r=0.581, p<0.001), ASDAS-ESR (r=0.604, p<0.001) and Ankylosing Spondylitis Disease Activity Score(ASDAS)- C reactive protein (CRP) (r=0.575, p<0.001). HIMRISS in group B and C was significantly higher than that in group A: 29.38（17.00，40.94）vs 14.50（11.38，22.25）, p=0.009; 38 (31.13，64.38）vs 14.50（11.38，22.25), p<0.001.Conclusions. HIMRISS applied to patients with AS demonstrated a satisfactory reliability, which means it is a reliable quantitive assessment tool for evaluating early hip involvement in patients with AS.
First Detection and Genetic Characterization of Equus Caballus Papillomavirus 1, 2 and 7 in China
Authors: Tong Panpan , Song Xiaozhen , Ren Meiling , Jia Erken , Zhang Lei , Palidan Nuerlan , Duan Ruli , Jia Chenyang , Kuang Ling , Wang Jinquan , Xie Jinxin 
Published: February 2021

Abstract
Background: Nine species of Equus caballus papillomavirus (EcPV) have been reported to infect horses, however, there are so far no reports of such infections in China. Results: In our pioneer study with Chinese horses, we found EcPV-1 in intranasal papilloma and nasal swabs, EcPV-2 in nasal swabs and semen, and EcPV-7 primarily in semen. This indicates that EcPVs are indeed hosted by horses in China, and that EcPV-2 and 7 may be getting transmitted though breeding. Sequence analyses for complete genomic sequences of EcPV-1 (G2), EcPV-2 (XJ-KS1391) and EcPV-7 (XJ-zs1) were performed which indicated that EcPV-1, 2 and 7, that infect horses in China, share 99.3% nt identity with the already published sequences for EcPV-1, 2 and 7. These observations indicate that three types of EcPVs identified in the current study are highly similar variants of previously known types of EcPV-1, 2 and 7. Phylogenetic analysis based on L1 genes in GenBank showed that EcPV-1, 2 and 7, found in Chinese horses, are closely related to and clustered together with already known EcPV-1, 2 and 7, respectively. Conclusion: Our study provides a novel evidence for EcPVs infection and circulation in Chinese horses and thus lays the foundation for a systematic and detailed epidemiological study of these infections in Chinese horses.
Structural, Morphological and Thermal Decomposition Studies of Calcium and Hafnium Modified BaTiO3 Electro Ceramics
Authors: Fernandez Jude , Bindhu B , Prabu M. , Sandhya KY 
Published: February 2021

Abstract
Calcium and hafnium co-doped barium titanate could be used as a replacement for lead zirconate titanate, which is a lead-based ferroelectric material. Solid state reaction accompanied by the usual sintering technique is the classical ceramic-processing method, which demands a lot of time and effort. The present work aims to make the process a lot easier and quicker by employing a modified sol-gel combustion technique to synthesize polycrystalline Ba0.85Ca0.15Ti(1-x)HfxO3 (x=0.00, 0.05, 0.10, 0.15) electro ceramics . The molar ration is fixed at 1:1 for metal and citric acid at pH ~ 1. It was found that Ba0.85Ca0.15Ti(1-x)HfxO3 (where x=0.00, 0.05, 0.10, 0.15) crystallizes completely at around 1000 °C which is much lower than traditional methods. The structure supposedly displays a tetragonal symmetry with the P4mm space group as confirmed through x-ray diffraction (XRD) and Raman spectroscopy.
Higher Mortality from Covid19&nbsp;in Patients with Renal Dysfunction in A Multicultural Multi-Ethnic Population
Authors: Nair Satish Chandrasekhar , Gasmelseed Huda Imam , Khan Asad Afroz , Khafagy Ibrahim Nageh , Abdrhman Hashim Ibrahim , Saleem Aqeel Aziz , Sreedha Jayadevan , Alhosani Ahmed Husain , Siddiqua Amatur Rahman , Ahmed Amna Riaz , Shubbar Aya Imad , Farajallah Majd Munir , Aleissaee Abdul Rahman , Hamadeh Alan Mohammad , Bashir Khlood Mustafa , Choker Haneen Bassam , Alzaabi Mohamed Nasir , Alshehhi Saif Saeed , Alblooshi Maitha Ali , Safdani Fatmah Ali , Shil Rajish Sanjit , Habbal Fuad Wardan , Alanqar Abdulrahman Wael , Douleh Wafa Fayez 
Published: February 2021

Abstract
Background

Despite previous exposure to coronavirus epidemics a few years ago, limited clinical and epidemiological information is available from the United Arab Emirates (UAE). The UAE is the second most affected country amongst the Gulf Cooperation Council Countries by Covid19. Distinctly, the UAE has a population of almost 9.2 million, with fewer than 12% UAE nationals, and the rest immigrants, mainly unskilled labourers. The disparate socio-economic structure, crowded housing conditions, multi-ethnic, multicultural population offers a unique set of challenges in Covid19 disease management.
Methods

In order to assess patient characteristics and survival for patients infected with Covid19, electronic patient data was retrospectively abstracted from the medical records of two designated public Covid19 referral hospitals, and subjected to statistical analysis.
Results

From, the total of 3072 patients, less than one-fifth were females, the Asian population (71.2%), followed by Middle Eastern Arabs (23.3%) were the most infected by the virus. Mortality was low among the Asian population. Diabetes Mellitus (26.8%, p < 0.001), hypertension (25.7%, p < 0.001), and heart disease (9.6%, p < 0.01) were the most prevalent comorbidities observed and decreased survival by 2–3 fold. Kidney disease escalated mortality rate by almost eight-fold high (19.4%, p < 0.001), as compared to patients without kidney disease. Higher age of patients between 51 and 65 years, significantly decreased the odds for survival (Crude OR 14.1, p<,0.001) and (Adjusted OR 12.3, p < 0.001), and patient age beyond 66 years, further significantly decreased the odds for survival (Crude OR 36.1, p < 0.001) and (Adjusted OR 26.6,p < 0.001). Kidney disease as comorbidity significantly diminished the survival rates (Crude OR 9.6,p < 0.001) and (Adjusted OR 5.7, p < 0.001), as compared to those without kidney dysfunction.
Conclusion

Although Asian population was the highest infected by Covd19, their mortality rate was low (2.6%), compared to other nationalities. Older ages above 51 years decreased the odds of survival significantly. Despite other comorbidity risks, kidney dysfunction contributed to enhanced mortality by over eight-fold and reduced the odds of survival (Adjusted OR 26.6), compared to those patients without kidney dysfunction. Our findings are important in the management of the Covid19 disease in the region with similar economic, social, cultural and ethnic background.
Robotic Radical Prostatectomy at the Egyptian National Cancer Institute: Overcoming the Challenges in the Initial Case Series
Authors: Zaghloul Ashraf Saad , Abdelbary Ahmed , Fergany Amr , Aboulkassem Hatem , Fadlalla Waleed Mohamed 
Published: February 2021

Abstract
Background

Robotic prostatectomy is showing progressive worldwide spread owing to potential clinical benefits, but at a higher cost.
Objective

This article describes the challenges and clinical outcome of the first group of patients undergoing robotic prostatectomy in Egypt.
Design, setting, participants

From 2017 to 2019, the data of all (55) patients undergoing robotic radical prostatectomy at the National Cancer Institute of Egypt was analyzed.
Outcome evaluation

Short term operative outcomes, complications, technical difficulties, pathologic data and biochemical recurrence were reported.
Results

Average blood loss was 296 ml; one patient required blood transfusion. One case required open conversion, another required re-docking of the robot. Setup time was significantly improved from an average of 27.7 minutes in the first 27 cases to an average of 17.3 minutes in the final 28 cases (p < 0.0001). Complications developed in 27% of our patients. Continence recovery at catheter removal, first, third, sixth and twelfth months were 32.7%, 50.9%, 65.5%, 74.5%, and 96%, respectively.
Conclusions

Results from the first series of robotic radical prostatectomy were encouraging. Technical challenges can be overcome in a short period. Acceptable complication rate and satisfactory outcomes regarding continence and blood loss were observed.