bluuebunny/crossref_metadata_2025_split huggingface dataset:
Recognizing the life of Aborigin Australia before the Europeans arrival
Authors: Sumardi  , Putri N E 
Published: May 2021

Abstract
This article seeks to provide understanding or information related to the life of Aboriginal people in Australia before the arrival of Europeans, known as pre-colonialism. As it is known, the continent of Australia was once occupied by Britain, which was originally a place for the laying of prisoners because at that time the prisons in England were full. Aboriginal is the name for the indigenous people who inhabited Australia for the first time. Aboriginal is the name of a tribe that was given by accident by the Europeans who saw it for the first time in Australia and this name applies to the designation of indigenous Australians to this day. The name of the Aboriginal tribe is a gift from Europeans who visited Australia and were surprised to see that the continent was inhabited. The term “Abo” means an astonishment of European society, and “origin” means authentic. The origin of the Aboriginal people is believed to have come from Asia who later occupied Australia for 45,000-50,000 years. The description of Aboriginal people is almost the same as the physical characteristics of the Papuans, the Toala people in Sulawesi, the Sakai people in Malaysia, the Veddas people in Sri Lanka, and the indigenous tribes of South India (Siboro, 1989). Aboriginal life before the arrival of Europe was rough and still very primitive. The way of life that is one with nature as well as various Aboriginal traditions and cultures is very interesting because it takes full advantage of the existing natural systems. For this reason, the study of Aboriginal people is very interesting.
Permaculture gardening, the first step of food sustainability in the tropical freshwater wetland
Authors: Krisdianto K , Susilawati I O , Fatimah S 
Published: May 2021

Abstract
Climate change has been causing severe damage in Kalimantan, such as forest fires in the dry season and flooding in the monsoon rain has been understood. Both of these results in loss of social-economic properties and environmental assets, and possible to create a climate emergence, especially in wetlands. The research about how the local people use traditional ways to revive their food and income through Permaculture garden in wetland has been conducted from 2018 – 2020. This research aims to elucidate the local people capability in use their local wisdom, traditional knowledge and ecological attitude on providing food in the transition of the wetland environment, through implementation of Permaculture concepts, learning and interaction.. About sixty voluntary respondents involve in forum group discussion, deep interview, and Permaculture gardening. The result shows that informal and local unique interaction among the local in implementing traditional, ecological knowledge and attitude in Permaculture gardening able to produce vegetables and fruits for daily, monthly and annual income. Thus, the observation of the implementation and the interaction on the concept of Permacultures may be possible to ensure us about the local sustainable food provision.
Water balance analysis and hydraulic structure design to prevent peatland fires
Authors: Assidik M L , Soekarno I , Widyaningtias  , Humam I A 
Published: May 2021

Abstract
Land fires have become a new phenomenon in Indonesia that has occurred continuously every year and on a large scale since 2010. Land fires mostly occur on peatlands during the dry season and the focus of this research is to find the solution for fire problem in peatlands. In this case, a hydrological approach is carried out to explain the causes of the fires that occur, and a solution is also made using this scientific approach. Many peatland fires occured when the groundwater level on the peat reaches 0.4 m below the surface of the land. Then a water balance analysis was carried out to calculate the potential for water in peatlands and also the water needed for evapotranspiration and other needs. After obtaining the amount of water demand, a long storage design with a volume of 1,097,344 m 3 was planned which was divided into six reservoirs. Long storage was designed with a transverse length of 35 m in the canals and with runners that have a height of 4 m from the riverbed. With storage in long storage, it was hoped that the existing water can be used in an effort to rewet peatlands and prevent potential fires.
Public services in a complete systematic land registration program (Study at Sungai Ulin Sub-District office, Banjarbaru City)
Authors: Saifudin  , Rafiqoh P A H 
Published: May 2021

Abstract
Referring to Ministerial Regulation No. 12 of 2017 concerning to Complete Systematic Land Registration program and the latest Presidential Instruction No. 2 of 2018 about concerning the Acceleration of Complete Systematic Land Registration with the aim of completing the registration of 79 million land fields that do not yet have guaranteed ownership of land rights. Then this study aims to know about how public services performed by Sungai Ulin Sub District Office for Complete Systematic Land Registration program by using a descriptive qualitative approach. Searching for data by direct observation in the field, this research is more directed towards services in completing the measurement of land field by referring to Zeithaml’s theory of 5 dimensions of indicators in assessing service quality, namely 1. Tangible (physical) in the form of convenience of service places, the ease of service processes, discipline of officers, the use of service aids, and the appearance of the officer. 2. Reliability in the form of accuracy of officers, clear services, and expertise of officers. 3. Responsiveness (response) in the form of responsiveness, accuracy, accuracy, and response to complaints. 4. Assurance in the form of guarantees on time, guarantee costs, and guarantees legality. And the last 5. Empathy, which is to prioritize customer interests, friendliness, and non-discrimination.
Correlation of students’ environmental literacy and scientific literacy after students’ involvement in wetlands-based stem educational approach
Authors: Syahmani  , Sauqina  , Hafizah E 
Published: May 2021

Abstract
Environmental Literacy is considered as one of the few perspectives necessary for SDGs to be achieved. At the same time, the concept of environmental literacy is often discussed with the concept of scientific literacy as basic requirement of environmental literacy. Scientific literacy is defined as the knowledge and understanding of scientific concepts and processes required for personal decision making, participation in civic and cultural affairs, and economic productivity. This study investigated the correlation between students’ environmental literacy and students’ scientific literacy after their scientific literacy has been improved using wetlands-based STEM educational approach. The results shows’ that there is no significant correlation between students’ scientific literacy and environmental literacy (ρ =0.085; sig.=0,519; α=0,05). This study discusses how environmental literacy should be a joint effort between educators from all different fields for it to be cultivated within student’s life.
Biodiversity of lactic acid bacteria of wild honey bee and Sumbawa horse milk by using RAPD-PCR
Authors: Prastyowati A , Mustopa A Z , Faiz M , Manguntungi A B , Fatimah  , Fidien K A 
Published: May 2021

Abstract
Wild honey bee and Sumbawa horse milk is the local product from West Nusa Tenggara, Indonesia, which has the characteristic of little sour in taste. The sour taste indicates lactic acid bacteria (LAB) existence that has a potential function as probiotics. The objection of this study is analysis of lactic acid bacteria biodiversity from wild honey bee and Sumbawa horse milk by using Random Amplified Polymorphic DNA-Polymerase Chain Reaction (RAPD-PCR). The genome of cultured samples from M17 and MRS medium were extracted, then identified by RAPD-PCR with primer (GTG)5 (5-GTGGTGGTGGTGGTG-3). The scoring of DNA fragments was analyzed using the Numerical Taxonomy and Multivariate Analysis System (NTSYS) application. The result show five clusters from a common ancestor indicated the broad diversity of LAB in wild honey bee and Sumbawa horse milk.
Traditional uses of herpetofauna practiced by local people in the island of Sumatra, Indonesia: Implications for conservation
Authors: Mardiastuti A , Masy’ud B , Ginoga L N , Sastranegara H , Sutopo  
Published: May 2021

Abstract
Local people in Sumatra have long utilized herpetofauna to provide food, medicine, and other purposes. However, research related to wildlife utilization is still lacking. The objective of this paper was to provide an overview of the island-wide (i.e. Sumatra) traditional use of herpetofauna by the local people and analyze its implications for conservation. Published papers were collected and analyzed. From 11 papers, 22 wild-caught species (18 reptiles, 4 amphibians) were used by local people for their meat (water monitor, freshwater turtles, large-sized snakes), eggs (marine and freshwater turtles), or medicinal purposes (snakes, lizards, frogs; as skin-related remedies). Among them, 8 reptiles are listed in the IUCN Red List and CITES Appendices, including Batagur borneoensis (CR, Appendix II), Chelonia mydas (EN, Appendix I), Notochelys platynota (VU, Appendix II), Ophiophagus hannah (VU, Appendix II), Ortilia borneensis (EN, Appendix II). Additionally, 5 reptiles are protected by Indonesian law. Batagur borneoensis is also listed as a very high priority species for conservation by the Ministry of Environment and Forestry. No amphibian is protected by law, listed in the CITES, nor IUCN Red List. Traditional uses of protected species and listed under the IUCN Red List need to be managed properly to ensure their sustainability.
Bird species contested at songbird competition in Jabodetabek Region, Indonesia
Authors: Angguni T , Mulyani Y A , Mardiastuti A 
Published: May 2021

Abstract
Songbird contests have been widely held in various regions lately, indicating the high interest of community towards this type of contest. The objectives of this study were to identify bird species contested in Jakarta, Bogor, Depok, Tangerang and Bekasi (Jabodetabek) region, classify them based on their conservation status, and collect other information related to bird conservation efforts. Of 20 bird contests that took place during the research period (March-May 2018) it was revealed that there were 15 species of birds (12 local species, 3 foreign species) that were contested. The species that was always contested in every event was Lovebird, while other frequently contested species (>50%) were Greater Green Leafbird, White-rumped Shama, Oriental Magpie-robin, and Canary. There was one protected species, i.e., Greater Green Leafbird that was contested. Based on the IUCN threat status, one vulnerable species was contested, i.e., Greater Green Leafbird. Near threatened species contested were Chestnut-capped Thrush and Lovebird. Based on CITES category, one species listed in Appendix II was found, i.e., Hwamei, which is a foreign species. Efforts made by the organizers of bird contests in bird conservation were to hold a class of rings (markers), to ensure that the contested birds originate from captive breeding.
Population, economic value and the trade of Dalbergia latifolia in Lombok, West Nusa Tenggara, Indonesia
Authors: Atikah T D , Wardani W , Surya D 
Published: May 2021

AbstractDalbergia latifoliais a valuable rosewood timber species locally known as sonokeling. This species is naturally found in India and Indonesia even though some reported it was cultivated. Like other rosewood, this species is mainly used for musical instruments, carving, or furniture. Since allDalbergiaspecies listing in CITES Appendix II following COP 17, international trade of products derived from this species must be controlled through a licensing system in accordance with CITES provisions. This permit granting must be based on a set of scientific-sound information, such as distribution, population size, and trade system. This research aims to present the economic value and trade system of sonokeling timber through survey and interview in Lombok, West Nusa Tenggara. The population of the standing stock was varied, between 12-34 individual/400m2. Most of the local farmers treat the species as an additional source of income following its rising economic value since the listing. Previously, people use it only for firewood that traded with some low prices. The change has brought the provincial government to impose a regulation for sonokeling trade activities in West Nusa Tenggara.
Hydrogel patch from liquid smoke and vitamin K Collard Greens extract for wound healing applications
Authors: Saputra S , Wibisono G , Ramadhani E , Dewi T 
Published: May 2021

Abstract
Wound care by using wound dressing has been evolved available in various physical forms including hydrogels with the highest global use reaching 43%. Nowadays, hydrogels are available at high cost because produced from synthetic materials, so natural materials are needed to obtain antibacterial wound dressing. This study aims to analyze the hydrogel patch PVA/chitosan/starch infused liquid smoke and vitamin K collard greens extract which are antibacterial effects and accelerates wound healing. This study was a laboratory experiment. Collard greens were extracted by using the Direct Solvent Extraction II method. The membrane characteristic tested using SEM test. The parallel streak method (AATCC 147-2004) was used for antibacterial testing. The hydrogel patch application was carried out on 28 male Rattus norvegicus strain wistar rats. They were given incisions wound and treated by a control group and 3 treatment groups and conducted wound healing analysis on the 4th,7th,11th and 14th day observation. The antibacterial test showed the hydrogel patch infused liquid smoke with a concentration of 12% was the optimum concentration inhibiting S. aureus. In conclusion, the hydrogel patch has a smooth and porous surface, can be inhibited the growth of S. aureus, and proven to completely heal the animal wound.
Detection of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) in clinical samples using Real-Time Reverse Transcription Polymerase Chain Reaction (qRT-PCR)
Authors: Atikana A , Sukmarini L , Hariyatun  , Ridwanulah A M , Nugroho H A , Sushadi P S , Juanssilfero A B , Agustiyanti D F , Putra M Y , Ningrum R A , Lisdiyanti P 
Published: May 2021

Abstract
The coronavirus disease 2019 (COVID-19) is caused by an infection with the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), which has infected more than 400,000 people, causing a worldwide pandemic. As of August 15, 2020, as many as 111,455 cases of COVID-19 have been confirmed in Indonesia with 6,071 deaths that have been recorded thus far. Quantitative real-time reverse-transcription polymerase chain reaction (qRT-PCR) has been used as a gold standard to detect the infection of SARS-CoV-2 in many clinical samples due to its sensitivity and specificity. In Indonesia, one of the recommended and commonly used diagnostic kit for COVID-19 was MiRXES Fortitude Kit 2.0E under the license of Singapore’s Agency for Science, Technology, and Research (A*STAR), Singapore. Aiming to assess the basic analytical and clinical performance of the MiRXES Fortitude Kit 2.0E, this study prepares serial dilutions of the provided positive control to establish PCR efficiency and to estimate the limit of detection. Moreover, a panel of SARS-CoV-2-positive-clinical samples (nasopharyngeal swab specimens) was tested to preliminarily evaluate the kit’s sensitivity on clinical samples. This study is expected to provide valuable assessment information for laboratories that develop routine diagnostic protocols for COVID-19, especially those using the MiRXES Fortitude Kit (A*Star, Singapore).
Capture and intermediate purification of human insulin precursor from Pichia pastoris culture using cation exchange chromatography
Authors: Putro E W , Nurdiani D , Hariyatun  , Utami N , Kusharyoto W 
Published: May 2021

Abstract
Purification has an important role in obtaining protein with a high degree of purity, particularly for human therapeutic purposes. Purification of pharmaceutical proteins requires several unit operations, involving chromatographic separation techniques. To increase purification efficiency and shorten process development, it is necessary to examine the chromatography system in performing a capture and intermediate purification in a single step. We use one of the best Pichia pastoris clones obtained from previous studies to produce a human insulin precursor (HIP). To capture and purify HIP from the culture, we clarified the cells through centrifugation and filtration. The supernatant was then loaded into a cation exchange column. Purification was carried on by two-step elution and monitored based on UV absorbance. Effects of loading concentration, flow rate, and pH of samples were evaluated. Fractions of elution were collected and verified by SDS-PAGE. Concentrations of HIP protein were quantified by using ImageJ by incorporating lysozyme as standard and reversed phase HPLC. Loading concentration and pH of the sample have an impact on the recovery. In this study, the best HIP recovery at ∼ 47% resulted from purification with 10% volume of loading concentration and 500 ml loading volume.
Increase in vitamin levels to improve shoot growth of diploid, triploid and tetraploid taro cultured in liquid medium
Authors: Wulansari A , Ermayanti T M , Purwito A , Sukma D 
Published: May 2021

AbstractTaro (Colocasia esculenta(L.) Schott) is one of important food crop in many countries including Indonesia, which has big diversity of taro germplasm. Tissue culture is anin vitrotechnique to mass-propagated propagules. Modification of the medium compositions is required to obtain the best growth of plantlets. This research was aimed to accelerate growth of taro shoot cultures by increasing of the vitamin levels added on MS liquid medium using three different ploidy levels of taro. Experiments were designed by increasing three kind of vitamin B i.e. thiamine, pyridoxine and nicotinic acid to 100-fold of the normal concentrations. Shoots were cultured for 4 weeks. Growth was determined by recording shoot numbers, petiole length, leaf and root numbers of culture. The results showed that increase in vitamin B levels affected growth of taro culture. Increase in nicotinic acid gave the best petiole length for Bentul diploid (5.39 cm), increase in pyridoxine concentration enhanced root numbers (6.7 roots). For Satoimo triploid, increase in thiamine combined with nicotinic acid gave the best for root numbers (8.7 roots). For Bentul tetraploid, increase in pyridoxine accelerated shoot numbers (1.3 shoots). Growth of Satoimo triploid was the best compared to both Bentul diploid and tetraploid.
Growth response of shallot (Allium ascalonicum L.) seedlings cultured on MS solid and liquid medium supplemented with BAP, Thiamine and Adenine Sulphate
Authors: Rantau D E , Wulandari D R , Maharijaya A 
Published: May 2021

Abstract
True Shallot Seeds (TSS) is the best choice of cultivation over bulb materials because it offers disease-free plants, produces larger size bulbs, and reduces cost production. In vitro propagation is an alternative method to overcome the TSS development problems. This research aimed to evaluate the growth response of shallot (Allium ascalonicum) cv Tuktuk seedlings, cultured on MS solid and liquid media with agitation supplemented with BAP, Thiamine and Adenine Sulphate. The solid MS medium was used to germinate TSS, followed by growth seedlings at control and treatment media. The experiments were carried out in a Completely Randomized Design with 4 replicates. Data were collected 8 weeks after planting. In vitro germination results showed that 5.96% of seeds started to germinate at the first week and reached 19.82% after four weeks of planting. After 8 weeks in the culture of TSS seedlings, shoots had better growth on solid medium compared to in liquid medium. The addition of BAP, Thiamine and Adenine did not significantly influence the growth of seedlings culture. Bulbs-like bodies were formed in all media. A higher concentration of plant growth regulators might be needed to enhance in vitro propagation of TSS seedlings.
Selection of highly productive clove trees in the Anambas population
Authors: Bermawie N , Wahyuni S , Ginting R 
Published: May 2021

Abstract
The productivity of cloves in Indonesia is low, probably due to the use of inferior seeds. The success of plant cultivation is determined by many factors, including the use of quality seeds. This study aims to select high-yielding clove trees, which can be recommended as a quality source of seeds. The research was conducted in Anambas Islands Regency, in a population of cloves belonging to a farmer. A highly productive population was selected as the genetic material following the guidelines for selecting the clove population. Furthermore, highly productive mother trees were selected from this population with the criteria of production average 80 kg tree−1. In addition to their production potential, their morphological characters and chemical compound content were also observed. Twenty mother trees with a production potential of more than 100 kg of flowers tree−1 were selected as high yielding trees. The morphological characters of the selected trees do not resemble any of the released varieties. The content of essential oils and main chemical components is following the Indonesian National Standard of clove quality. Twenty highly productive trees can be recommended for further research for use as sources of quality seeds.
Phytochemical compounds identification of three bajakah species (Salacia sp., Uncaria acida, and Uncaria gambir) using GC-MS pyrolysis
Authors: Paramita P P , Tata H L 
Published: May 2021

Abstract
Bajakah is a woody-liana known for traditional medicine by the Dayak and Banjar tribes in Central Kalimantan. Three bajakah, which were identified as Salacia sp., Uncaria acida, and Uncaria gambir, were collected from their natural habitats in peatland hydrological unit of Sebangau–Kahayan rivers. GC-MS pyrolysis was used to analyze the identity of phytochemical compounds in the wood and bark of bajakah. The results showed that phytochemical compounds were varied both in the woods and barks which ranges from 30 to 40 types, with the largest component is phenol. Seven phytochemical compounds were not identified. Phenolic compounds varied among 3-8 types. The next step was determining total flavonoid and phenol content using Folin-Ciocalteu method. Results showed that bark of Uncaria acida has the highest total phenol and flavonoid content, which were 0.013% and 0.028%, respectively. Wood of Salacia sp. has the lowest total phenolic and flavonoid content, which were 0.013% and 0.028%, respectively. The commercial bajakah that are usually sold in the market, which was unidentified, has medium total phenolic and flavonoid content, which are 0.010% and 0.025%, respectively. In summary, the composition and content of phytochemical compounds in bajakah were determined by the species.