bluuebunny/crossref_metadata_2025_split huggingface dataset:
256 Effects of Transition Period Nutrition on Uterine Health
Authors: Cardoso Felipe 
Published: October 2021

Abstract
The transition from gestation to lactation, also known as the transition period, is a critical time for dairy cows. This phase is typically defined as 3 wk before parturition through 3 wk after parturition. Peak disease incidence (shortly after parturition) corresponds with the time of greatest negative energy balance (NEB), the peak in blood concentrations of nonesterified fatty acids, and the greatest acceleration of milk yield. Decreased fertility in the face of increasing milk production may be attributable to greater severity of postpartal NEB resulting from inadequate transition management or increased rates of disease. The depth and duration of NEB is highly related to dry matter intake. Formulation and delivery of appropriate diets that limit total energy intake to requirements but also provide proper intakes of all other nutrients (including the most limiting amino acids Met and Lys) before calving can help lessen the extent of NEB after calving. Cows that received controlled-energy diets during the last 3 wk prepartum had shorter days to pregnancy than cows that consumed high-energy diets in this time period. Additionally, a fully acidified prepartum diet formulated with a higher Ca concentration improved reproductive performance and uterine immune function in the postpartum period through the decrease of days to first ovulation, a tendency to decrease service per conception rate, improved glandular morphology, a tendency to increase polymorphonuclear neutrophil infiltration, and a tendency to increase the tight-junction protein occludin. Supplementation of rumen-protected methionine during the transition period resulted in improved uterine immune function through improved glandular morphology, increased neutrophil infiltration number after calving, and discovery of neutrophil extracellular trap formation in bovine endometrial tissue. In conclusion, nutritional strategies during the transition impact uterine health and fertility in dairy cows.
265 Awardee Talk: Maternal and Paternal Contributions to Fertility
Authors: Pohler Ky G , Reese Sydney T , Franco-Johannsen Gessica A , de Melo Gabriela Dalmaso , Filho Ramiro Oliveira , Poole Rebecca K 
Published: October 2021

Abstract
Using over 56,000 pregnancy diagnostic records in beef cattle, a meta-analysis was conducted to objectively determine that 28.4 % of embryos will not develop past day 7 of gestation with most embryonic losses occurring before day 4. Furthermore, by day 30 of gestation, 47.9 % of cows submitted to a single insemination at day 0 will not be pregnant and pregnancy loss between days 32 and 100 was 5.8%. Reproductive success, however, is highly variable and influenced by maternal and paternal factors. Maternal characteristics, including subspecies, parity and reproductive tract size, intersect with breeding management decisions regarding estrus expression and detection to influence conception and pregnancy rates. The maternal endocrine environment, from estradiol associated with estrus around fertilization to elevated prostaglandins during the embryonic to fetal transition period, play critical roles in pregnancy success. Despite the numerous maternal factors involved with pregnancy establishment, sire contribution to reproductive failure should not be overlooked. Sires are reported to influence pregnancy loss during the second month of gestation in both beef and dairy herds. These differences in sire field fertility could not be explained by differences in semen characteristics nor differences in sire conception rate (SCR) score. To understand parental contribution to pregnancy development during the second month of gestation we developed parthenogenetic (PA) embryos (embryos lacking paternal genome). We observed that PA conceptus had well developed trophectoderm tissue at day 31 of gestation but no site of embryo attachment to the endometrium. Moreover, conceptus secretion such as pregnancy-associated glycoproteins and interferon-stimulated genes were not found in maternal circulation. These results suggest that paternal genetics is required for post-elongated embryo attachment to endometrium. Further exploring the maternal vs paternal contribution to pregnancy development can help elucidate the mechanism that drives reproductive failure in cattle.
268 Growth Performance of Weaned Angus Bulls Housed in a Compost-bedded Pack Barn
Authors: Harrelson Flint W , Harrelson Patricia L , Branham Courtney , Meyers Anna 
Published: October 2021

Abstract
Compost-bedded pack barns (CBP) are commonly utilized in dairy cattle operations across the United States. Their popularity is the result of improved animal welfare and reduced costs. The crossover of CBP into the beef cattle industry is new and the benefits and downfalls are not yet explored. Through a grant from NRCS, a CBP was built at the Morehead State University Derrickson Agricultural Complex in 2019–2020. In fall of 2020, the pack was established by mixing manure and sawdust shavings to start the aerobic composting process. The objective of our study was to determine the performance of growing bulls utilizing a CBP compared to a fully outdoor pasture housing system and measure the pack development. Growth data from 2 consecutive years of growing bulls were utilized, with calves born in 2019 being used as a control (CON) group and calves born in 2020 being used as the CBP group. Adjusted weaning and yearling weights reported to the American Angus Association were utilized to measure growth performance. Heifers born in both years were also evaluated as an outside standardization mechanism to eliminate genetic bias. Temperature measurements, surface and internal, were collected 3 times weekly to measure pack establishment. Weaning weights for CON and CBP bulls were similar (P = 0.45), though interestingly the corresponding heifer calves weaned with CBP bulls were heavier (P &lt; 0.01) than those weaned with the CON bulls. Bulls housed in the CBP were significantly heavier (P &lt; 0.01) as yearlings compared to the CON bulls (496 kg vs. 425 kg, respectively). Temperature differed (P &lt; 0.02) between day, side of the barn, and measurement location. Our results suggest that utilizing a compost bedded pack barn during the winter growing phase may be beneficial to the producer by increasing growth potential of weaned calves.
285 Effects of Encapsulated Calcium-ammonium Nitrate on Greenhouse Gas Emissions from Manure of Beef Steers Grazing a Mature Mixed-winter Forage
Authors: Henry Darren D , Osorio Andrea M , Mejia-Turcios Sebastian E , Vargas David A , Slaughter Lindsey C , Kohmann Marta , Ciriaco Francine M , Onorino Sarturi Jhones O , DiLorenzo Nicolas 
Published: October 2021

Abstract
A 30-d experiment was conducted to evaluate daily and cumulative gas fluxes of N2O, CH4, and CO2 produced by manure from Angus-crossbred steers grazing mature mixed-winter forage pastures [wheat, triticale, and rye (Triticum aestivum, Triticosecale rimpaui, and Secale cereal, respectively) and receiving N supplementation from two different sources. Steers received the following treatments: 1) mature mixed-winter pasture + ground corn (NCTRL), 2) NCTRL + 328 mg/kg of BW encapsulated calcium-ammonium nitrate (NIT) and 3) NCTRL + 124 mg/kg of BW urea (CTRL). All ground corn was supplemented at 0.3% BW. Treatments NIT and CTRL were isonitrogenous. Feces were collected and composited (1 kg as-is) within treatment, within block (3 blocks; 4 steers/treatment/block; 3 fecal composites/treatment). Gas samples were collected from static chambers previously installed in an area excluded from grazing. After 3 d, composites were deposited on the soil surface inside the chamber. Four subsamples were taken per deployment time per chamber, separated by 10-min intervals (t0, t10, t20 and t30) and injected into an evacuated 125-mL serum vial. Gas samples were collected every other day between 0900 and 1100 h and analyzed using a gas chromatograph. Data were analyzed as a randomized complete block design, with chamber as the experimental unit, using the MIXED procedure of SAS. No treatment × day interaction (P ≥ 0.145), nor treatment (P ≥ 0.622) effect were observed on daily-flux data for N2O, CH4, and CO2; however, a day effect was observed (P ≤ 0.001) where all gases peaked on d 2 post-manure application on the soil. Cumulative emissions were not different among treatments for N2O, CH4, and CO2 (P ≥ 0.663). Although it was expected for encapsulated calcium-ammonium nitrate to increase N2O emissions, such effect was not observed. Therefore, it appears that encapsulated calcium-ammonium nitrate does not affect manure greenhouse gas emissions.
282 Nutritional Monitoring of Prenatally Stressed and Translocated Brahman Heifers
Authors: Tolleson Doug R , Randel Ron D , Perry George A , Diaz Jose M , Starns Heath D , Knight Colt W , Garza Nick E , Beall Sarah L , Neuendorff Don A , Welsh Thomas H 
Published: October 2021

Abstract
One drought mitigation strategy is transporting livestock to non-drought locations. Our objective was to evaluate the effects of prenatal stress and translocation on growing Bos indicus heifers. Twelve heifers born in spring 2019 at Overton, TX (1245 mm annual precipitation) were transported ~700 km to Sonora, TX (610 mm annual precipitation) in April 2020. Six heifers (283±10 kg) were born to dams subjected to transportation stress during mid-gestation (PNS) and 6 (279±17 kg) were born to non-stressed dams (CON). Heifers grazed a series of 24-ha native range pastures (aboveground forage biomass; 1508±390 kg/ha) and were sampled (non-shrunk BW/BCS score, feces) at 2-wk intervals from May through September. Fecal samples were collected from the ground or the rectum of each animal and stored at -20o C until processed for near infrared spectroscopy (NIRS) and prediction of diet crude protein (CP) and digestible organic matter (DOM). Inputs to a grazing animal nutrition model for prediction of BW included diet CP and DOM, age, and weather. Differences between groups for BW and nutritional parameters were determined by analysis of variance or paired t-test. Both groups gained BW (22±4 kg) throughout the study, there were no differences (P &gt; 0.1) due to treatment. Diet CP and DOM were affected by date (P &lt; 0.01) as diet quality declined from spring to fall. Percent diet CP was greater (P &lt; 0.05) in PNS than CON, especially during July and August (6.94±0.10 vs 6.23±0.17, respectively). Corresponding values for diet DOM were (59.53±0.55 vs 59.14±0.43, respectively; P = 0.09). Observed vs model-predicted weight was different (P &lt; 0.05) for both groups when using CP-based outputs, but not when using metabolizable protein-based outputs (P &gt; 0.1). In summary, PNS heifers selected a diet of greater CP than CON, and tended to select a diet greater in DOM.
528 Late-Breaking: Late Gestation Metabolizable Energy Intake Increases Colostrum Yield and Alters Colostrum Composition in Beef Cattle
Authors: Hare Koryn S , Croft Emily , Wood Katharine M , Steele Michael A 
Published: October 2021

Abstract
The objective was to determine how late gestation metabolizable energy (ME) intake impacts beef cow colostrogenesis. Angus-Simmental primiparous (n = 47) and multiparous (n = 109) cattle were blocked by expected calving date and randomly assigned to receive diets that supplied 80% (LME; n = 53), 100% (CME; n = 52), or 120% (HME; n = 51) of the ME requirement. Treatments were balanced by initial BW and fed for 53 d prior to calving. Cow body weight was recorded d -53, -39, -25, -10, and -3 relative to calving and retrospectively conceptus-corrected. Calves were weighed and measured prior to suckling. Colostrum was hand-stripped from all cows for compositional analysis and a subset (n = 16/treatment) was fully milked using a milking machine. Data were analyzed (PROC GLIMMIX) as a randomized block design, including the fixed effects of treatment, parity, and treatment×parity and the random effects of block and cow(block). Day and its interactions were included for repeated measurements. Treatment did not affect (P ≥ 0.73) cow BW or conceptus-corrected BW. Calf chest circumference was lesser (treatment×parity: P = 0.023) for primiparous vs. multiparous calves if their dams consumed LME or CME, but not if their dams consumed HME. Feeding HME rather than LME increased (P = 0.006) colostrum yield. The LME diet increased (P = 0.004) colostrum IgG concentration over HME; however, HME colostrum yielded more (P = 0.005) total IgG. Crude protein and urea-N were elevated (P &lt; 0.001) in LME colostrum, whereas HME colostrum contained more (P = 0.026) lactose. Low ME colostrum fat was greater (P = 0.013) than CME and beta-hydroxybutyrate was increased (P &lt; 0.001) in LME vs. CME and HME colostrum. These data provide insight regarding nutrient partitioning during late gestation and demonstrate that beef cow colostrogenesis is responsive to prepartum ME intake.
522 Late-Breaking: Effects of Corn Processing and Age on Energy Balance, Nutrient Digestion and Rumination in Cattle
Authors: Petzel Emily , Brake Derek 
Published: October 2021

Abstract
Current library values for energy content in whole or dry-rolled corn (DRC) are identical and do not differ for cattle of different age (NASEM, 2016). Calves (295 ± 29 kg) and yearlings (521 ± 29 kg) were fed whole, DRC or steam-flaked corn (SFC) to evaluate library values of energy content in different types of processed corn among cattle of different age. Cattle were fed diets comprised of 75% corn (DM-basis) to 2.5-times maintenance energy requirements using estimates of NEm in corn and SFC (NASEM, 2016). Data were analyzed using the MIXED procedure of SAS. There was no observed interaction of corn processing and cattle age (P ≥ 0.40). Time spent ruminating (min/d) was not different between dietary treatment or age. Total tract starch digestibility was greatest (P = 0.01) for cattle fed SFC (97.5%), intermediate in cattle fed DRC (92.4%) and least in cattle fed whole corn (89.5%). Nitrogen balance was not affected (P ≥ 0.30) by corn processing or age. Digestible and metabolizable energy (Mcal/kg-DMI) were greater (P ≤ 0.05) for cattle fed SFC compared to DRC or whole. A greater proportion of DE was lost to heat production (P = 0.01) in cattle fed whole corn compared to DRC and tended to be greater (P = 0.08) in cattle fed SFC than DRC. Retained energy (Mcal/d) should have been similar if current library values correctly reflect energy content in each type of processed corn. However, retained energy was greater (P &lt; 0.01) for cattle fed DRC compared to whole corn and tended to be greater (P = 0.06) compared to SFC indicating that library values for DRC underestimate energy available to growing cattle. Measures of retained energy for cattle fed DRC indicated that energy available for gain from DRC was 42% greater than library values (NASEM, 2016).
527 Late-Breaking: Objective Assessment of Behaviour, Pain, and Lameness Due to Digital Dermatitis in Beef Cattle
Authors: Thomas Anice D , Orsel Karin , Pajor Edmond 
Published: October 2021

Abstract
Digital dermatitis (DD) is an infectious disease affecting cattle feet causing ulcerative and necrotic skin lesions. Most lesions are painful when palpated, some cause lameness and significantly impact animal welfare and productivity. Our objectives were: to determine if changes in behaviour were associated with DD in cattle, to quantify pain associated with DD lesions, and to determine the relationship between pain sensitivity and lameness, and DD and lesion temperature. In total, 255 animals across three feedlots were enrolled. A subset of 120 animals were fitted with accelerometers to record behaviour. Cattle were scored for lameness (Step-Up Locomotion Scoring System), both hind feet scored for DD (M-stage scoring system), the pressure pain threshold of lesions determined using pressure algometry and lesion temperature using thermal images. Animals and feet were classified as DD absent or present. Further, DD present was divided into active or chronic. Behaviour, 5 to 2 days before DD identification was analyzed. Animals with DD ruminated 3% less daily compared to animals without DD (P &lt; 0.05). Further, animals with active lesions ruminated 5% less daily (P &lt; 0.05). Daily inactivity time was greater in animals with DD and the effect of day depended on type of lesion (P &lt; 0.05). Feet without DD lesions withstood 5.6N more pressure (P &lt; 0.001). Active lesions were most sensitive withstanding 8.1N less pressure and chronic lesions 4.1N less pressure than DD absent feet (P &lt; 0.05). Moderate to severely lame animals withstood 3.1N less pressure than sound animals (P &lt; 0.001). Median maximum temperature (MMT) was higher in feet with DD compared to those without (P &lt; 0.001) with MMT being highest in active lesions (P &lt; 0.05). In conclusion, rumination is depressed, and inactivity increased in DD affected animals. Both active and chronic lesions are painful, lame animals have a lower pain threshold and DD is associated with higher lesion temperatures.
519 Late-Breaking: Evaluation of the 7 & 7 Synch and Bee Synch II protocols for control of the estrous cycle among cows of varying percentage Bos indicus influence
Authors: Andersen Carson M , Holton Matthew P , Fontes Pedro L , Thomas Jordan M , Goncalves Lucas , Burato Samir 
Published: October 2021

Abstract
The 7 & 7 Synch and Bee Synch II protocols for control of the estrous cycle were compared among cows with varying percentages of Bos indicus influence. Angus (n = 48), ⅛ - ¼ Brahman-influenced (n = 90), Brangus (n = 11), and ½ Brahman-influenced (n = 56) cows were blocked based on breed, age, and days postpartum and randomly assigned to treatment. Cows treated with 7 & 7 Synch (n = 104) received prostaglandin F2α (PG; 25 mg dinoprost tromethamine) and insertion of an intravaginal progesterone-releasing insert (CIDR; 1.38g progesterone) on Day -17, gonadotropin-releasing hormone (GnRH; 100 μg gonadorelin) on Day -10, and PG coincident with CIDR removal on Day -3. Cows treated with Bee Synch II (n = 101) received PG coincident with CIDR insertion on Day -8 and PG coincident with CIDR removal on Day -3. Fixed-time artificial insemination was performed on Day 0 at 66 h. Ovarian ultrasound was performed on Day -3 to assess presence of corpora lutea (CL) and diameter of the largest ovarian follicle (LFD), and color Doppler was used on Day 7 to assess CL blood perfusion. A greater proportion of 7 & 7 Synch cows (P &lt; 0.0001) had CL on Day -3 (83%; 86/104) compared with Bee Synch II cows (48%; 48/101). On Day -3, LFD did not differ among Bos indicus-influenced cows treated with Bee Synch II (12.5±0.3 mm), Bos indicus-influenced cows treated with 7 & 7 Synch (12.2±0.3 mm), or Angus cows treated with 7 & 7 Synch (12.1±0.6 mm); however, greater LFD was observed among Angus cows treated with Bee Synch II (15.4±0.8 mm). Brangus cows had greater periphery signal percentages (P = 0.02) when treated with 7 & 7 Synch compared with Bee Synch II. In conclusion, across cow of varying percentage Bos indicus influence, 7 & 7 Synch resulted in a greater proportion of cows with CL on Day -3.
520 Late-Breaking: Effect of Technology on Environmental Emissions from Four Representative U.S. Feedlots and the Beef Production System
Authors: Gilreath Jessica , Rotz Al , Place Sara , Thoma Greg , Wickersham Tryon 
Published: October 2021

Abstract
Our objective was to evaluate effects of technological management strategies on environmental impacts and net returns of feedlot operations in the United States. Feedlot operations were simulated with the Integrated Farm System Model (IFSM 4.6; USDA-ARS, University Park, PA) to quantify baseline environmental impacts of feedlot production and full US beef cattle production systems. Strategies simulated included: ionophore, implant, ractopamine hydrochloride, combined management (ionophore, implant, and ractopamine hydrochloride; I+I+R), lubabegron, reduced mortality rate, and improved fiber digestion. Days on feed were adjusted whenever necessary and according to production practices typical of commercial feedlots. Subsequently, annual number of cattle finished by the operation was adjusted according to days on feed to maintain consistent one-time capacities. Mitigation strategies were individually modeled and simulated in IFSM for each feedlot operation to calculate intensities (expressed per kg gain) for greenhouse gas (GHG) emissions, fossil energy use, blue water consumption, and reactive nitrogen loss. Additionally, net returns to management were estimated for each feedlot operation. Feedlots were then integrated with simulations of cow-calf, stocker, and backgrounding operations to estimate environmental intensities (expressed per kg carcass weight) for the full beef cattle production system. Carbon emission intensity was reduced most using the I+I+R strategy (10%), followed by implant (6%) and ionophore (5%) strategies alone. Similarly, energy use intensity was reduced the greatest by I+I+R (9%), ionophore (5%), and implants (4%). Reductions in water use intensity were also greatest using I+I+R (9%). Net returns increased for all strategies compared to baseline net return with the greatest improvements observed for I+I+R ($114/finished animal) and implants ($66/animal). Consistent results were observed for all strategies simulated across all four environmental intensities when quantified for the full cattle production system. Implementing I+I+R (7%), ractopamine hydrochloride (4%), and lubabegron (4%) in feedlots resulted in the greatest reductions in environmental emissions.
295 Effects of Live Yeast and Ruminal Environment on Beef Cattle Diet Digestibility: An in vitro Approach
Authors: Scaranto Silva Kaliu G , Onorino Sarturi Jhones O , Henry Darren D , Karges Kip , Hinds Jordan K , Tonelli Nardi Kaue T , Fávero Igor Gomes , Saes Yasmin , Rush Camron J 
Published: October 2021

Abstract
The effects of live yeast (Saccharomyces cerevisiae) and ruminal environment on in vitro true digestibility of dry matter, organic matter, and fiber components were evaluated. Ruminally-cannulated beef steers (n = 4; BW = 520 ± 30 kg) were used in a cross-over design to serve as donors of ruminal content. In vitro substrates (6 grower and 6 finisher diets) and individual ingredients (steam-flaked corn, wet corn gluten feed, and old-world bluestem hay) were dehydrated (55o C), ground (1 mm), and incubated using a 2 × 2 factorial arrangement of treatments: a) the presence or not of yeast (8×109 CFU/animal-daily adjusted to a 70 L ruminal content volume), and b) two ruminal inoculums collected from donors fed a grower (50:50) or a finisher (92:08) diet (concentrate:forage). Four incubation batches (experimental unit) were performed, in which the initial two batches were performed followed by another two after animals switched diets and were adapted again during 21d. A Daisy in vitro incubation system was used, and samples incubated for 48h (39°C). The GLIMMIX procedure of SAS was used for the statistical analysis. No 3-way interactions (P ≥ 0.71) or 2-way interactions with substrate were observed (P ≥ 0.25). The in vitro true digestibility of DM, OM, NDF, and hemicellulose increased when yeast was added to the incubator containing a 50:50 ruminal content (Rumen × Yeast; P ≤ 0.02). The ADF digestibility increased (P = 0.02) with yeast addition regardless of ruminal content type (50:50 or 92:08). As expected, finisher substrate diets were more (P &lt; 0.01) digestible than grower diets. The combination of live yeast and a specific ruminal environment seemed to be more important than substrate type, in which live yeast improved in vitro digestion within a 50:50 ruminal inoculum more evidently.