bluuebunny/crossref_metadata_2025_split huggingface dataset:
Abnormal Epigenetic Modifications in Surviving Somatic Cell Cloned Cattle Associated With Donor Cell Type
Authors: Ma Haoqiang , Huang Tian , Zhou Jin , Li Jinghui , Cui Shenghui , Ye Chongyuan , Lu Chao , Bai Yueyu , Cao Gengsheng 
Published: September 2021

Abstract
Background

Studies have shown that the efficiency of somatic cell nuclear transfer (SCNT) is related to the type of donor cell used. Previous studies have shown that fetal oviduct epithelial cells (FOVs) exhibit a higher blastocyst formation rate than fetal fibroblasts (FFBs), but they are associated with lower pregnancy, calving, and full-term rates after implantation. The reason for this difference is unclear.
Result

In this study, we performed the assay for transposase-accessible chromatin with high-throughput sequencing (ATAC-seq), RNA-seq, and 5-hydroxymethylcytidine (5hmC) and 5-methylcytosine (5mC) DNA methylation sequencing methods across the whole genome to analyze the epigenetic differences between cattle cloned using FFBs or FOVs as donor nuclei. The results showed that chromatin openness, gene expression levels, and 5hmC contents were greater in cloned cattle derived from FOV donor nuclei than in those derived from FFBs. ATAC-seq and RNA-seq analyses of cloned bovine ear tissues derived from the same source of donor nuclei showed an obvious clustering tendency. In this study, we also found that the 5hmC content of surviving cloned cattle derived from FFBs was greater than 4‰, whereas it was less than 2‰ in dead cloned cattle.
Conclusion

We found that there were abnormalities in specific epigenetic modifications and gene expression in living somatic cell cloned cattle derived from different donor nuclei. Although cloned cattle undergo somatic reprogramming and differentiation, they retain the epigenetic imprints of their donor nuclei, and this somatic imprinting may affect the development rate of cloned blastocysts as well as the birth rate and development status of cloned fetuses after implantation.
Development of a Lifestyle Intervention Program to Support Preconception, Pregnant and Postnatal Women Who are Overweight or Obese: A Qualitative Study
Authors: Ku Chee Wai , Leow Shu Hui , Ong Lay See , Erwin Christina , Ong Isabella , Ng Xiang Wen , Tan Jacinth JX , Yap Fabian , Chan Jerry Kok Yen , Loy See Ling 
Published: September 2021

Abstract
Background: Poor lifestyle behaviors, including unhealthy diet and physical inactivity, contribute to the global obesity pandemic and result in long-term adverse health effects on mothers and their children. The time period before, during and after pregnancy represents a unique opportunity for interventions to cultivate sustained healthy lifestyle behaviors. Since the success of a lifestyle intervention is heavily dependent on uptake and continued compliance, the intervention components should be acceptable, sustainable, and tailored to the concerns and needs of the target population. This study aims to identify enablers and barriers towards engagement with a lifestyle intervention for improving the metabolic health of prospective mothers and their offspring, among a sample of the target population.Methods: In-depth interviews were conducted with 15 overweight or obese women in the preconception, pregnancy or postpartum periods. Interviews were transcribed verbatim and thematic analysis was undertaken using NVivo. Factors influencing adoption of a novel lifestyle intervention were systematically charted using the integrated–Promoting Action on Research Implementation in Health Services (i-PARIHS) framework, that comprises Recipient, Context, Innovation and Facilitation constructs.Results: Ten enablers and barriers were identified. Barrier factors within the Recipient construct include time constraints as participants juggled multiple roles, and poor baseline knowledge about healthy behaviors and the impact of maternal obesity on maternal and offspring health. Within Context, a family culture of communal meals, convenient access to unhealthy foods in the environment, as well as lack of relevant data sources, were barriers to dietary change. Recipient motivation to be healthy for themselves and their offspring is an enabler, along with family and social support within the Context construct. In the Innovation construct, a holistic delivery platform providing desired information delivered at appropriate times increases engagement. Lastly, in Facilitation, regular feedback, goal setting and nudges would ensure continued engagement and sustainability of lifestyle changes.Conclusions: The features of a successful lifestyle intervention targeting overweight and obese women include (i) a holistic life-course approach to provide education and guidance, (ii) using mobile health platforms to reduce barriers, provide personalized feedback and promote goal-setting, and (iii) health nudges to cultivate sustained lifestyle habits.
Dexmedetomidine Inhibits Nlrp3 Inflammasome Priming in BV-2 microglia through the NF-κB Pathway and the ROS-Nlrp3-IL-1β Signaling Axis
Authors: Xie Jiawei , Chen Li , Luo Yuling , Li Jianling , Wang Xianxue , Tang Jieke , Tian He , Liang Shuqing , Xi Biao , Li Yalan 
Published: September 2021

Abstract
In the brain, the NOD-like receptor family pyrin domain containing 3 (Nlrp3) inflammasome is mostly expressed in microglia and is considered to be the primary cause of perioperative neurocognitive dysfunction (PND). Dexmedetomidine (Dex), a novel kind of clinical anesthetic with anti-inflammatory properties, has been shown to be effective in preventing PND in surgical patients. However, the mechanism of its anti-neuroinflammatory activity is still quite unclear. We examined the impact of Dex administration on Nlrp3 priming in activated BV-2 cells in this research. To investigate the mechanism by which Dex impacts Nlrp3 priming, we employed the inhibitors pyrrolidine dithiocarbamate (PDTC) and N-acetyl-L-cysteine (NAC) to block the NF-κB p65 and the reactive oxygen species (ROS)-Nlrp3-interleukin (IL)-1β signaling axis, respectively. The results showed that Dex substantially decreased the expression of Nlrp3 and p65 and significantly inhibited the levels of the inflammatory factors IL-1β and tumor necrosis factor (TNF)-α in BV-2 cells stimulated with lipopolysaccharide (LPS). Additionally, when the NF-κB pathway was inhibited by PDTC, Dex could aggravate the downregulation of Nlrp3 and IL-1β in BV-2 cells. What is more, Dex negatively regulated the expression of Nlrp3 and IL-1β in activated BV-2 cells when NAC was added. These results showed that Dex inhibited Nlrp3 priming in LPS-induced BV-2 cells, presumably via blocking the NF-κB pathway and the ROS-Nlrp3-IL-1β signaling axis.
Psychometric Properties of The Persian Version of Short-Form Five Factor Borderline Inventory (FFBI-SF)
Authors: Athar Mojtaba Elhami , Jazi Elham Azamian , Lashgari Zahra , Azizi Morteza , Karimi Sirvan , DeShong Hilary L. 
Published: September 2021

Abstract
Background
The Five-Factor Borderline Inventory-Short Form (FFBI-SF) is a self-report measure developed to assess traits of Borderline Personality Disorder (BPD) from the perspective of the Five-Factor Model of general personality. This study was designed to examine the factor structure, internal consistency, and convergent/discriminant validity of the Persian FFBI-SF in a sample of Iranian university students.
Methods
A total of 641 university students (M-age = 28.04, SD = 8.21, 66.7% women) completed the online forms of the FFBI-SF, PID-5-BF, and Mini IPIP.
Results
Confirmatory factor analysis supported the proposed twelve-factor model. Also, Cronbach's alpha (α) for the FFBI-SF scores ranged from unacceptable to excellent ranges. However, when relying on MIC values to measure internal consistency, the FFBI-SF Total and subscale scores demonstrated adequate internal consistency. Finally, the FFBI Total and subscale scores showed the expected relations with external correlates (e.g., Neuroticism, Antagonism, and Conscientiousness), which supports the validity of the interpretation of the FFBI-SF scores.
Conclusions
The findings indicated that FFBI-SF is a useful tool with sound psychometric properties for assessing BPD traits in Iranian students and may spark research in other Iranian settings (e.g., clinical samples).
Advanced Paternal Age With Risk of Lifespan in Male Offspring Using Chinese Genealogical Data
Authors: xiong yanyan , Zhu Xueqin , Xie Ying 
Published: September 2021

Abstract
Background Advanced paternal age has been associated with a variety of adverse reproductive outcomes. However, the effects of paternal age on offspring lifespan are still controversial. Result Here, we studied the correlation between parental reproductive age and offspring lifespan using a Chinese specific genealogy data. We chose a “Ding” genealogy data across 130 years during the Qing Dynasty (1726-1855). The present study showed that fathers aged more than 35 years were more likely to have male offspring with lower lifetime compared with fathers aged 20-35 years. The significant negative correlation between paternal age and male offspring lifespan existed after adjusted for maternal age. We proposed a new evidence that advanced paternal age is a risk for male offspring survival.
Risk Prediction of Hepatocellular Carcinoma in Patients With Alcoholic Cirrhosis in an Area With Intermediate Prevalence for Hepatitis B Virus Infection: a Competing Risk Analysis
Authors: Lee Kyunghan , Choi Gwang Hyeon , Jang Eun Sun , Jeong Sook-Hyang , Kim Jin-Wook 
Published: September 2021

Abstract
Background & Aims: The role of hepatocellular carcinoma (HCC) surveillance is being questioned in alcoholic cirrhosis because of the relative low incidence of HCC. Comorbid viral hepatitis may modify the risk, and competing outcomes may influence the actual incidence of HCC in alcoholic cirrhosis. This study aimed to assess the risk and predictors of HCC in patients with alcoholic cirrhosis by using competing risk analysis in an area with intermediate prevalence for hepatitis B virus.Methods: A total of 965 patients with alcoholic cirrhosis were recruited at a university-affiliated hospital in Korea and randomly assigned to either the derivation (n=643) and validation (n=322) cohort. Subdistribution hazards model of Fine and Gray was used with deaths and liver transplantation treated as competing risks. Death records were confirmed from Korean government databases. A nomogram was developed to calculate the Alcohol-associated Liver Cancer Estimation (ALICE) score.Results: Markers for viral hepatitis were positive in 21.0 % and 25.8 % of patients in derivation and validation cohort, respectively. The cumulative incidence of HCC was 13.5 and 14.9 % at 10 years for derivation and validation cohort, respectively. Age, positivity for viral hepatitis markers, alpha-fetoprotein level, and platelet count were identified as independent predictors of HCC and incorporated in the ALICE score, which discriminated low, intermediate, and high risk for HCC in alcoholic cirrhosis at the cut-off of 120 and 180. Conclusions: ALICE score reliably stratifies HCC risk of alcoholic cirrhosis in an area where the prevalence of viral hepatitis is substantial.
The Impact of Online Design Thinking-Based Learning On Entrepreneurial Intention: The Case of Vocational College
Authors: Woraphiphat Issariya , Roopsuwankun Pattama 
Published: September 2021

Abstract
Entrepreneurial intention has been found to be fundamental to decision-making and behavior to become entrepreneurs, with consequent effects on economic development. However, the COVID-19 pandemic calls for a novel approach to teaching entrepreneurship owing to the shift to online learning. The current study explores entrepreneurial intention derived from the entrepreneurship education program (EEP) using an online group-based learning approach. In particular, we examine satisfaction with an online group-based learning approach by integrating the theory of planned behavior with design thinking-based entrepreneurship course, peer interactions, and speaker interactions. The entrepreneurship education program (EEP) in this research was for vocational college students located in Southeast Asia. The online questionnaire was distributed to participants (N = 263, MAGE = 18.64) at the end of the online entrepreneurship education program. The model was tested using a structural equation model analysis. Attitude, subject norm, and satisfaction were found to predict higher entrepreneurial intention among vocational college students. Moreover, design thinking-based entrepreneurship course, peer interaction, and speaker interaction have indirect effects on entrepreneurial intention through satisfaction. This research extends the literature on entrepreneurship education by proposing a novel learning approach, that is, the online groupbased learning approach, which could be applied to entrepreneurship education programs to enhance students’ entrepreneurial intention.
Transfer of Cleavage-Stage Embryos May Benefit Normal Responders With Low Rate of Morphologically Good Embryos Formation On Day 3: A Comparison Study
Authors: Dai Xiuliang , Xia Xiyang , Gao Tingting , Yu Chunmei , Cao Fang , Li Tianfu , Li Lingjun , Wang Yufeng , Chen Li 
Published: September 2021

Abstract
Background
Do morphologically good (MG) embryos from patients with high and low rate of MG embryos on day 3 (RMD3) show similar developmental potential (DP)?
Methods

This respective study finally included a total of 916 fresh cycles and related 1074 FET cycles from Jan 2017 to May 2020 in our reproductive center. Cycles with high RMD3 were defined as the H group, while cycles with low RMD3 were defined as the L group. The basic characteristics of patients and fresh cycles, blastulation rate, and clinical outcomes were compared between the H and L groups in either ET cycles with MG day 3 cleavage embryos (ETC group) or ET cycles with MG blastocysts (ETB group).
Results

The overall characteristics of patients and cycles were grossly comparable between the H and L groups either in ETC or ETB groups. In ETB group, useable blastocysts formation rate, implantation rate and live birth rate was significantly reduced in the L group, compared to the H group;In ETC group, useable blastocysts formation rate was significantly reduced in the L group. However, implantation rate and livebirth rate was similar between the L and H groups.
Conclusion

The in vitro DP of MG day 3 embryos and in vivo DP of MG blastocysts were reduced significantly, while a similar in vivo DP of MG day 3 embryos was observed in patients with low RMD3 as compared to patients with high RMD3. It seems that direct transfer of day 3 MG embryos instead of extended culture may benefit patients with RMD3.
Factors Affecting People’s Environmental Awareness In The Urban Areas: A Case of Addis Ababa, Ethiopia
Authors: Getachew Dagne , Assefa Engdawork , Seyoum Abrham 
Published: September 2021

Abstract
Background: In developing countries, the urban environment is deteriorating over time. In the meantime, people's demand for clean and green residential and recreational places has increased. If so, why has it been hard to keep clean and green cities? This paper investigates the level and determinants of environmental awareness in Addis Ababa. From three sub-cities, a three-stage sampling procedure applies to select 293 sample respondents. The data collection applies a structured questionnaire. We applied a five-point Likert scale to classify the levels of awareness. Besides, an ordered logit model was applied to analyze factors that affect the level of awareness.Results: The result shows that the knowledge of forest degradation is high, while the attitude to reduce the use of glass bottles is medium. The cognitive skill on the cause of acid rain is medium. The probability of low (13%) and medium (26%) levels of environmental awareness increases for the income group of 601 to 1650. Likewise, the likelihood of having low levels of environmental awareness rises by 9%; in contrast, the probability of having a moderate level of environmental awareness increases by 12% for the age of 50 to 59. The TVET educational level has a low chance of having low (8%) and medium (12%) levels of awareness.Conclusions: An income-generating activity raises employment opportunities and creates a better income, which would influence the environmental mindset. So, improving the living standard assures clean and green cities. Besides, the higher the education, the better would be environmental knowledge, cognitive skills, and attitude. In the meantime, besides formal education, adult education, training, and workshops are alternatives to enhance environmental awareness.
The Impact of Design Elements on Undergraduate Nursing Students’ Educational Outcomes in Simulation Education: Protocol for a Systematic Review
Authors: Jackson Matthew , McTier Lauren , Brooks Laura A , Wynne Rochelle 
Published: September 2021

Abstract
Background: Although simulation-based education (SBE) has become increasingly popular as a mode of teaching in undergraduate nursing courses its effect on associated student learning outcomes remains ambiguous. Educational outcomes are influenced by SBE quality that is governed by technology, training, resources and SBE design elements. This paper reports the protocol for a systematic review to identify, appraise and synthesise the best available evidence regarding the impact of SBE on undergraduate nurses’ learning outcomes. Methods: Databases to be searched from 1st of January 1990 include the Cumulative Index to Nursing and Allied Health Literature (CINAHL), the Medical Literature Analysis and Retrieval System Online (MEDLINE), American Psychological Association (APA) PsycInfo and the Education Resources Information Centre (ERIC) via the EBSCO host platform. The Excerpta Medica database (EMBASE) will be searched via the OVID platform. We will review the reference lists of relevant articles for additional citations. A combination of search terms including ‘nursing students’, ‘simulation training, ‘patient simulation’, and ‘immersive simulation’ with common Boolean operators will be used. Specific search terms will be combined with either MeSH or Emtree terms and appropriate permutations for each database. Search findings will be imported into reference management software (Endnote© Version.X9) then uploaded into Covidence where two reviewers will independently screen titles, abstracts and retrieved full text. A third reviewer will be available to resolve conflicts and moderate consensus discussions. Quantitative primary research studies evaluating the effect of SBE on undergraduate nursing students’ educational outcomes will be included. The Mixed Methods Appraisal Tool (MMAT) will be used for quality assessment of core criteria, in addition to the Cochrane RoB 2 and ROBINS-I to assess risk of bias for randomised and non-randomised studies, respectively. Primary outcomes are any measure of knowledge, skills, or attitude. Discussion: SBE has been widely adopted by healthcare disciplines in tertiary teaching settings. This systematic review will reveal (i) the effect of SBE on learning outcomes, (ii) SBE element variability, and (iii) interplay between SBE elements and learning outcome. Findings will specify SBE design elements to inform the design and implementation of future strategies for simulation-based undergraduate nursing education.Systematic Review Registration: PROSPERO CRD42021244530
Mapping of Natural Radioactivity in Soil Samples of Kufa Districts, Iraq Using GIS Technique
Authors: Alasadi Lubna A. , Abojassim Ali Abid 
Published: September 2021

Abstract
The aim from this work is to detect and draw the natural radioactivity of (uranium-238, thorium-232 and potassium-40) levels in soil samples that taken from Kufa districts, Najaf governorate, Iraq. The detection system used in study is NaI (Tl) with "3x3" dimension, while maps were drawn by geographic information system (GIS). Some radiological parameters were calculated depending on measuring of specific activity for 238U, 232Th, and 40K. The results of the average value with standard error in all samples of the present study for 238U, 232Th, 40K, and 235U were 6.2±0.74, 6.41±0.82, 278.10±19.43, and 0.28±0.03 Bq/kg, respectively. The specific activity of Uranium-238, Thourium-232, poasium-40 and Uranium-235 found in all soil samples were within the safety limit reported by UNSCEAR, except three samples have poasium-40 higher than safety limit. Also, the results of radiological parameters were all found to be below OECD, UNSCEAR safety limit. Generally, the average of specific activity in soil collected arranged of potassium-40 > thorium-232 > uranium-238. Mapping GIS for natural radioactivity and some radiological parameters were successfully draw for the study area. Finally, natural radioactivity and radiological parameters for soil samples at Kufa districts were safety.
Self-healing Co3O4@Fe3O4/cellulose Blend Membranes Efficient Degrade Perfluorooctanoic Acid in the Visible Light-driven Photo-fenton System
Authors: Chen Wenqiang , Hou Chen , Wang Yang , Fu Linhui , Zhang Sufeng , Wang jianzhi 
Published: September 2021

Abstract
Co3O4@Fe3O4/cellulose was synthesized by in-situ self-assembly strategy coating rod-like MOF-derived Fe3O4 with Co3O4 nanoparticles and blending with cellulose solution, further applied in the visible light-driven photo-Fenton system for PFOA degradation. In addition, Co3O4@Fe3O4/cellulose/Vitrimer was obtained to explore the application of self-healing property in photo-Fenton filed and the result turned out to be good self-healing capacity for small cracks. In comparison, Co3O4@Fe3O4/cellulose can degrade around 94.5% PFOA within 180 min in reaction system, which shows better degradation capacity than others catalyst. Moreover, Co3O4@Fe3O4/cellulose was reused by rinsing with ultra-pure water and the degradation capacity was still 80.4% after five cycles. In this system, the results of Electron paramagnetic resonance analysis (EPR) and scavenger experiment suggested that PFOA degradation was a co-dependent mechanism via photogenerated electrons, photogenerated holes (h+) and various radical species, rather than a single active constituent. The degradation pathway of PFOA also was proposed based on UHPLC-MS analysis.