bluuebunny/crossref_metadata_2025_split huggingface dataset:
Hoxb5 reprograms murine multipotent blood progenitors into hematopoietic stem cell-like cells
Authors: Huang Dehao , Zhao Qianhao , Weng Qitong , Zhang Qi , Wang Kaitao , Liu Lijuan , Xia Chengxiang , Wang Tongjie , Xiong Jiapin , Liu Xiaofei , Guan Yuxian , Geng Yang , Dong Fang , Cheng Hui , Wang Jinyong , Zhang Mengyun , Hu Fangxiao 
Published: October 2021

AbstractThe expression of transcription factor Hoxb5 specifically marks the functional hematopoietic stem cells (HSC) in mice. However, our recent work demonstrated that ectopic expression of Hoxb5 exerted little effect on HSC but could convert B cell progenitors into functional T cells in vivo. Thus, cell type- and development stage-specific roles of Hoxb5 in hematopoietic hierarchy await more extensive exploration. Here, with a mouse strain engineered with conditional expression of Hoxb5, we unveiled that induced expression of Hoxb5 in mouse multipotent progenitor cells (MPP) led to the generation of a de novo Sca1+cKit+Mac1+CD48+ (Mac1+CD48+SK) cell type, which has the ability to repopulate long-term multi-lineage hematopoiesis in serial transplant recipients. RNA-seq analyses showed that Mac1+CD48+SK cells exhibited an acquired machinery of DNA replication and cell division, which resembled nature fetal liver HSC cells (FL HSC). In short, our current study uncovers that Hoxb5 is able to empower MPP with self-renewal potential, thereby providing new strategies to reprogram blood progenitor cells into HSC-like cells.
Widespread sex ratio polymorphism inCaenorhabditisnematodes
Authors: Huang Yun , Lo Yun-Hua , Hsu Jung-Chen , Le Tho Son , Yang Fang-Jung , Chang Tiffany , Braendle Christian , Wang John 
Published: October 2021

AbstractAlthough equal sex ratio is ubiquitous and represents an equilibrium in evolutionary theory, biased sex ratios are predicted for certain local conditions. Cases of sex ratio bias have been mostly reported for single species, but little is known about its evolution above the species level. Here, we surveyed progeny sex ratios in 23 species of the nematode genusCaenorhabditis, including 19 for which we tested multiple strains. For the species with multiple strains, 5 species had female-biased and 2 had non-biased sex ratios in all strains, respectively. The other 12 species showed polymorphic sex ratios across strains. Female-biased sex ratios could be due to sperm competition whereby X-bearing sperm outcompete nullo-X sperm during fertilization. In this model, when sperm are limited allowing all sperm to be used, sex ratios are expected to be equal. However, in assays limiting mating to a few hours, most strains showed similarly biased sex ratios compared to unlimited mating experiments, except that oneC. beceistrain showed significantly reduced female bias compared to unlimited mating. Our study shows frequent polymorphism in sex ratios withinCaenorhabditisspecies and that sperm competition alone cannot explain the sex ratio bias.
The route of vaccine administration determines whether blood neutrophils undergo long-term phenotypic modifications
Authors: Feraoun Yanis , Palgen Jean-Louis , Joly Candie , Tchitchek Nicolas , Marcos-Lopez Ernesto , Dereuddre-Bosquet Nathalie , Gallouet Anne-Sophie , Contreras Vanessa , Lévy Yves , Martinon Frédéric , Le Grand Roger , Beignon Anne-Sophie 
Published: October 2021

ABSTRACTInnate immunity modulates adaptive immunity and defines the magnitude, quality, and longevity of antigen-specific T- and B- cell immune memory. Various vaccine and administration factors influence the immune response to vaccination, including the route of vaccine delivery.We studied the dynamics of innate cell responses in blood using a preclinical model of non-human primates immunized with a live attenuated vaccinia virus, Modified vaccinia virus Ankara (MVA), and mass cytometry. We previously showed that MVA induces a strong, early, and transient innate response, but also late phenotypic modifications of blood myeloid cells after two months when injected subcutaneously. Here, we show that the early innate effector cell responses and plasma inflammatory cytokine profiles differ between subcutaneous and intradermal vaccine injection. Additionally, we show that the intradermal administration fails to induce more highly activated/mature neutrophils long after immunization, in contrast to subcutaneous administration.Different batches of antibodies, staining protocols and generations of mass cytometers were used to generate the two datasets that were compared. Mass cytometry data were analyzed in parallel using the same analytical pipeline based on three successive clustering steps, including SPADE, and categorical heatmaps were compared using the Manhattan distance to measure the similarity between cell cluster phenotypes.Overall, we show that the vaccine per se is not sufficient for the late phenotypic modifications of innate myeloid cells, which are evocative of innate immune training. Its route of administration is also crucial, likely by influencing the early innate response, and systemic inflammation, and the vaccine biodistribution.
Eukaryotic CRFK cells motion characterized with atomic force microscopy
Authors: Zamora-Ceballos Maria , Barcena Juan , Mertens Johann 
Published: October 2021

AbstractWe performed a time-lapse imaging with Atomic Force Microscopy (AFM) of the motion of eukaryotic CRFK (Crandell-Rees Feline Kidney) cells adhered onto a glass surface and anchored to other cells in culture medium at 37°C. The main finding is a gradient in the spring constant of the actomyosin cortex along the cells axis. The rigidity increases at the rear of the cells during motion. This observation as well as a dramatic decrease of the volume suggests that cells may organize a dissymmetry in the skeleton network to expulse water and drive actively the rear edge.
Signal reconstruction and disturbance observer-based fault-tolerant control for civil aircrafts
Authors: Ma Xinjian , Liu Shiqian , Cheng Huihui , Lyu Weizhi 
Published: October 2021

Purpose
This paper aims to focus on the sensor fault-tolerant control (FTC) for civil aircraft under exterior disturbance.

Design/methodology/approach
First, a three-step cubature Kalman filter (TSCKF) is designed to detect and isolate the sensor fault and to reconstruct the sensor signal. Meanwhile, a nonlinear disturbance observer (NDO) is designed for disturbance estimation. The NDO and the TSCKF are combined together and an NDO-TSCKF is proposed to solve the problem of sensor faults and bounded disturbances simultaneously. Furthermore, an FTC scheme is designed based on the nonlinear dynamic inversion (NDI) and the NDO-TSCKF.

Findings
The method is verified by a Cessna 172 aircraft model under bias gyro fault and constant angular rate disturbance. The proposed NDO-TSCKF has the ability of signal reconstruction and disturbance estimation. The proposed FTC scheme is also able to solve the sensor fault and disturbance simultaneously.

Research limitations/implications
NDO-TSCKF is the novel algorithm used in sensor signal reconstruction for aircraft. Then, disturbance observer-based FTC can improve the flight control system performances when the system with faults.

Practical implications
The NDO-TSCKF-based FTC scheme can be used to solve the sensor fault and exterior disturbance in flight control. For example, the bias gyro fault with constant angular rate disturbance of a civil aircraft is studied.

Social implications
Signal reconstruction for critical sensor faults and disturbance observer-based FTC for civil aircraft are useful in modern civil aircraft design and development.

Originality/value
This is the research paper studies on the signal reconstruction and FTC scheme for civil aircraft. The proposed NDO-TSCKF is better than the current reconstruction filter because the failed sensor signal can be reconstructed under disturbances. This control scheme has a better fault-tolerant capability for sensor faults and bounded disturbances than using regular NDI control.
Selling science: optimizing the research funding evaluation and decision process
Authors: Vinkenburg Claartje J. , Ossenkop Carolin , Schiffbaenker Helene 
Published: October 2021

PurposeIn this contribution to EDI's professional insights, the authors develop practical and evidence-based recommendations that are developed for bias mitigation, discretion elimination and process optimization in panel evaluations and decisions in research funding. An analysis is made of how the expectation of “selling science” adds layers of complexity to the evaluation and decision process. The insights are relevant for optimization of similar processes, including publication, recruitment and selection, tenure and promotion.Design/methodology/approachThe recommendations are informed by experiences and evidence from commissioned projects with European research funding organizations. The authors distinguish between three aspects of the evaluation process: written applications, enacted performance and group dynamics. Vignettes are provided to set the stage for the analysis of how bias and (lack of) fit to an ideal image makes it easier for some than for others to be funded.FindingsIn research funding decisions, (over)selling science is expected but creates shifting standards for evaluation, resulting in a narrow band of acceptable behavior for applicants. In the authors' recommendations, research funding organizations, evaluators and panel chairs will find practical ideas and levers for process optimization, standardization and customization, in terms of awareness, accountability, biased language, criteria, structure and time.Originality/valueShowing how “selling science” in research funding adds to the cumulative disadvantage of bias, the authors offer design specifications for interventions to mitigate the negative effects of bias on evaluations and decisions, improve selection habits, eliminate discretion and create a more inclusive process.
Systematic mapping: educational and social entrepreneurship innovations (2015–2020)
Authors: Montes-Martínez Ruth , Ramírez-Montoya María Soledad 
Published: October 2021

PurposeThis study aims to analyze recent publications (2015–2020) that refer to educational and social entrepreneurship to identify the primary emerging themes and gaps of entrepreneurship research and management that would be helpful for future studies and entrepreneurial ventures.Design/methodology/approachThe authors used systematic mapping to review 92 research articles that address educational innovation and social entrepreneurship. All the articles were published between 2015 and January 2020 and were found in the Web of Science (WoS) and Scopus databases.FindingsThe data analysis identified the following: the articles most frequently cited, the journals that published the highest number of relevant articles, the geographical distribution of these publications and their authors, the context of the research, the lines and themes that emerged, and the gaps in the literature regarding the research and management of educational and social entrepreneurship.Research limitations/implicationsThe search for articles was limited to educational and social entrepreneurship innovations and the English language; thus, studies published in other languages were not analyzed.Originality/valueThe analysis of this research allowed us to review concepts and identify research methods employed and thematic lines analyzed. Therefore, the work is of value for educational and social entrepreneurs and researchers who wish to examine such concepts or focus on areas not yet fully explored.
A framework for scheduling IoT application jobs on fog computing infrastructure based on QoS parameters
Authors: Kaur Mandeep , Sandhu Rajinder , Mohana Rajni 
Published: October 2021

Purpose
The purpose of this study is to verify that if applications categories are segmented and resources are allocated based on their specific category, how effective scheduling can be done?.

Design/methodology/approach
This paper proposes a scheduling framework for IoT application jobs, based upon the Quality of Service (QoS) parameters, which works at coarse grained level to select a fog environment and at fine grained level to select a fog node. Fog environment is chosen considering availability, physical distance, latency and throughput. At fine grained (node selection) level, a probability triad (C, M, G) is anticipated using Naïve Bayes algorithm which provides probability of newly submitted application job to fall in either of the categories Compute (C) intensive, Memory (M) intensive and GPU (G) intensive.

Findings
Experiment results showed that the proposed framework performed better than traditional cloud and fog computing paradigms.

Originality/value
The proposed framework combines types of applications and computation capabilities of Fog computing environment, which is not carried out to the best of knowledge of authors.
Developing key competencies in sustainability through project-based learning in graduate sustainability programs
Authors: Birdman Jodie , Wiek Arnim , Lang Daniel J. 
Published: October 2021

Purpose
This research aims to investigate the role of project-based-learning within graduate sustainability curricula through the lens of key competence development. Project-based learning has become a widely recommended pedagogy for sustainability education. It is hypothesized that through collaboration, student autonomy and real-world application, students develop key competencies for sustainability. This paper also aims to examine the connection between project-based learning and competence development on a program level from the student perspective.

Design/methodology/approach
This two-year comparative case study follows the project-based-learning journeys of nine graduate sustainability students from three programs: the Master’s of Sustainability at Arizona State University, the Master’s of Sustainability Science at Leuphana University of Lüneburg and the Global Sustainability Science Master’s, an ASU and Leuphana collaboration. Over four semesters, the students each took part in four competence-oriented self-assessments and interviews to map their perceived learning throughout their programs. Additional contextual information was gathered from program and course materials and descriptions, instructor interviews and in vivo observations.

Findings
The defining aspects of project-based learning including collaboration, student autonomy and real-world connection do contribute to students’ self-perceived competence development. Student-driven and program-driven project-based learning experiences equally foster this result, as long as the pedagogical challenges of balancing support and student independence associated with each are mitigated through instructor actions, program design or individual student coping skills.

Originality/value
The results of this research can support higher education institutions in designing sustainability programs aimed at competence development through project-based learning. The focus on the curricular and program level combined with repeated overtime student-reported attribution to specific courses and activities bridges the gap between individual course case studies and theoretical recommendations for curriculum design. In addition to length and depth, this study also forefronts student experience of curricula as delivered.
AI-powered learning application use and gratification: an integrative model
Authors: Chang Younghoon , Lee Seongyong , Wong Siew Fan , Jeong Seon-phil 
Published: October 2021

PurposeAs the center of the fourth industrial revolution, artificial intelligence (AI) has marked its presence in various disciplines including the education field in the form of AI-powered learning applications. The purpose of this study is to build a research model capturing the relationships among use contexts, user gratification, attitude, learning performance and continuous intention to use an AI-powered English learning application.Design/methodology/approachUsing the use and gratification theory, use contexts and the belief-attitude-intention theory, this paper uses a quantitative approach based on a survey method for data collection and structural equation modeling for analysis. A total of 478 students from an international university in Guangdong, China, participated in the survey after using Liulishuo for two weeks.FindingsThe results showed that perceived use contexts affected all variables associated with gratifications-obtained and gratification-opportunities. With the exception of social integrativeness, all other gratification-based factors significantly affected attitude. The attitude in turn significantly influenced learning performance and continuous use intention.Originality/valueMobile AI-powered learning applications are at the center of research on technology-enhanced learning in the age of media and technology convergence. The study is timely and contributes to the discussion of the roles of use context and gratifications on technology users’ attitudes and behavioral intentions.
A retrospection of methodological pluralism in the Journal of Financial Management of Property and Construction (2005-2020)
Authors: Tembo Chipozya Kosta , Akintola Adeyemi 
Published: October 2021

Purpose
This paper presents a review of research methodologies used in addressing problems in the financial management of property and construction journals from 2005 to 2020.

Design/methodology/approach
Content analysis of 258 research papers published in the Journal of Financial Management of Property and Construction was carried out, enabling the exploration of research approaches, epistemology, strategies, data collection and data analysis methods used in addressing problems researched in the area of financial management of property and construction

Findings
The findings show that quantitative approaches and methods dominate, whereas qualitative and mixed methods were prominent in-depth understanding of a topics were needed. Interestingly, almost a third of the publications did not adopt quantitative approaches. In some journal issues, there was relatively high use of qualitative and multi-method approaches and up to 12% of the articles published over the past 16 years could be described as based on pragmatism.

Research limitations/implications
An important implication of this paper is that a conventionally number-based area of research does not preclude the use of qualitative and mixed approaches. The findings are only generalisable to the Journal of Financial Management of Property and Construction.

Practical implications
Financial management researchers could benefit greatly by considering pluralistic approaches more in the design of their studies.

Originality/value
To the best of the authors’ knowledge, this study is an original synthesis of the articles published between 2005 and 2020. It provides new insight into the use of research methodologies by authors and how they have been combined to address their research problems. It further investigates an old issue or question about methodological choice-making using new evidence and original empirical work.
Professional learning for secondary teachers of English learners in an urban school district: examining systems of support
Authors: Weddle Hayley , Yoshisato Mariko , Hopkins Megan 
Published: October 2021

PurposeAlthough schools across the United States are becoming increasingly linguistically and culturally diverse, many teachers remain underprepared to work with students classified as English learners (ELs), especially at the secondary level. Acknowledging the importance of developing systems of support for teachers of ELs, this paper examines the district- and school-level factors shaping secondary teachers' access to EL-focused professional learning in one large urban school district.Design/methodology/approachTo examine teachers' access to EL-focused professional learning, the authors draw on 49 in-depth interviews with district leaders and staff from nine secondary schools. Data analysis was guided by a structure, culture and agency theoretical framework.FindingsFindings revealed that decreased structural support, in terms of both fiscal and human resources, constrained teachers' access to EL-related professional learning. Further, the district culture was characterized by limited understanding of ELs' backgrounds and assets. While some school leaders exercised agency to bolster EL-focused professional learning for teachers, such supports were rare.Practical implicationsFindings help to contextualize secondary teachers' feelings of unpreparedness to serve ELs, illuminating several factors that district and school leaders should attend to in order to bolster the development of professional capital for teachers of ELs at the secondary level.Originality/valueWhile prior research outlines the importance of designing systems of support for EL-focused professional learning, this study highlights specific structural and cultural factors shaping such systems.
A study on the effects of electrical and thermal stresses on void formation and migration lifetime of Sn3.0Ag0.5Cu solder joints
Authors: Li Yanruoyue , Fu Guicui , Wan Bo , Wu Zhaoxi , Yan Xiaojun , Zhang Weifang 
Published: October 2021

Purpose
The purpose of this study is to investigate the effect of electrical and thermal stresses on the void formation of the Sn3.0Ag0.5Cu (SAC305) lead-free ball grid array (BGA) solder joints and to propose a modified mean-time-to-failure (MTTF) equation when joints are subjected to coupling stress.

Design/methodology/approach
The samples of the BGA package were subjected to a migration test at different currents and temperatures. Voltage variation was recorded for analysis. Scanning electron microscope and electron back-scattered diffraction were applied to achieve the micromorphological observations. Additionally, the experimental and simulation results were combined to fit the modified model parameters.

Findings
Voids appeared at the corner of the cathode. The resistance of the daisy chain increased. Two stages of resistance variation were confirmed. The crystal lattice orientation rotated and became consistent and ordered. Electrical and thermal stresses had an impact on the void formation. As the current density and temperature increased, the void increased. The lifetime of the solder joint decreased as the electrical and thermal stresses increased. A modified MTTF model was proposed and its parameters were confirmed by theoretical derivation and test data fitting.

Originality/value
This study focuses on the effects of coupling stress on the void formation of the SAC305 BGA solder joint. The microstructure and macroscopic performance were studied to identify the effects of different stresses with the use of a variety of analytical methods. The modified MTTF model was constructed for application to SAC305 BGA solder joints. It was found suitable for larger current densities and larger influences of Joule heating and for the welding ball structure with current crowding.