bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comparison of different detection methods for Ascaris suum infection on Austrian swine farms
Authors: Joachim Anja , Winkler Christian , Ruczizka Ursula , Ladinig Andrea , Koch Michaela , Tichy Alexander , Schwarz Lukas 
Published: October 2021

Abstract
Background
Ascaris suum, the large roundworm of pigs, is one of the economically most important pig parasites worldwide. In Austria it is commonly diagnosed by monitoring livers for milk spots at the slaughterhouse and intravital diagnosis (flotation for detection of fecal egg shedding). Recently, serological diagnosis based on the detection of specific antibodies with an ELISA (SERASCA®) with high sensitivity has been developed. To introduce and evaluate serology for A. suum screening in Austrian pigs, blood (for serology) (n = 177) and feces (for copromicroscopy) (n = 177) were taken from randomly selected slaughter pig batches from 18 farms at a slaughterhouse in Lower Austria. In addition, livers presented at slaughter (n = 844; max. 70/farm) were evaluated for milk spots.

Results
Overall, 19% of the livers were milk spot-positive (22% of those with complete diagnostic evaluations). Thirteen percent of the fecal samples contained A. suum eggs, while 69% of the blood samples were serologically positive. Despite we did not determine the sensitivity of the ELISA specifically, results ouf our study confirmed the high sensitivity of the ELISA, which was claimed by the manufacturer prior to our work (sensitivity: liver assessment: 23.5–27.0%; copromicroscopy: 8.5–9.0%; ELISA: 99.5%), and a high percentage of A. suum infections that remained undetected by standard liver assessment.

Conclusions
This suggests that the current method of roundworm diagnostics is insufficient and antibody detection at the end of the fattening period should be established as the standard procedure.
Adaptability and Resilience in Aging Adults (ARIAA): protocol for a pilot and feasibility study in chronic low back pain
Authors: Lysne Paige E. , Palit Shreela , Morais Calia A. , DeMonte Lucas C. , Lakdawala Maria , Sibille Kimberly T. , Bartley Emily J. 
Published: October 2021

AbstractBackgroundChronic low back pain (cLBP) is the leading cause of disability among older adults and one of the top reasons for seeking healthcare, resulting in significant decrements in physical functioning. Because older adults are among the fastest growing cohorts in the USA, both the incidence and burden of cLBP are expected to increase considerably, rendering geriatric pain management a top health priority. Resilience is defined as a process allowing individuals to adapt and recover from adverse and stressful conditions, and it has been highlighted as a crucial factor in positive health-related functioning. While a growing body of literature supports the use of resilience-based interventions in chronic pain, research examining their effectiveness in older adults with cLBP remains limited. The primary aims of the study are to assess the feasibility and acceptability of a psychologically oriented resilience intervention among aging adults with cLBP.MethodsIn this article, we describe the rationale and design of the Adaptability and Resilience in Aging Adults (ARIAA) study, a single-arm intervention in which 60 participants (ages ≥ 60 years) with cLBP will be recruited to participate in a 7-week group-based program aimed at enhancing psychological resilience. Intervention sessions will target positive psychology concepts (e.g., positive affect, pain acceptance, hopeful thinking, pain self-efficacy) and cognitive behavioral techniques that have established benefits in pain management. Primary study outcomes include intervention feasibility and acceptability as measured by treatment engagement, intervention credibility and satisfaction, ability to meet recruitment and retention metrics, and the feasibility of questionnaire and home activity completion. Outcomes will be assessed at baseline, immediately at posttreatment, and at the 3-month follow-up period.DiscussionThis study will establish the feasibility and acceptability of a novel intervention aimed at enhancing positive, psychological functioning, and resilience in older adults with cLBP. Achievement of these aims will provide a rich platform for future intervention research targeting improvements in pain and disability among geriatric populations and will serve as a foundation for a fully powered trial to examine treatment efficacy of the proposed intervention.Trial registrationClinicaltrials.gov, identifierNCT04068922. Registered 28 August 2019.
Design and 3D-printing of MRI-compatible cradle for imaging mouse tumors
Authors: Donohoe Deborah L. , Dennert Katherine , Kumar Rajeev , Freudinger Bonnie P. , Sherman Alexander J. 
Published: October 2021

Abstract
Background
The ability of 3D printing using plastics and resins that are magnetic resonance imaging (MRI) compatible provides opportunities to tailor design features to specific imaging needs. In this study an MRI compatible cradle was designed to fit the need for repeatable serial images of mice within a mouse specific low field MRI.

Methods
Several designs were reviewed which resulted in an open style stereotaxic cradle to fit within specific bore tolerances and allow maximum flexibility with interchangeable radiofrequency (RF) coils. CAD drawings were generated, cradle was printed and tested with phantom material and animals. Images were analyzed for quality and optimized using the new cradle.
Testing with multiple phantoms was done to affirm that material choice did not create unwanted image artifact and to optimize imaging parameters. Once phantom testing was satisfied, mouse imaging began.

Results
The 3D printed cradle fit instrument tolerances, accommodated multiple coil configurations and physiological monitoring equipment, and allowed for improved image quality and reproducibility while also reducing overall imaging time and animal safety.

Conclusions
The generation of a 3D printed stereotaxic cradle was a low-cost option which functioned well for our laboratory.
Epidemiology of Cryptosporidium sp. infection among free-range and intensive farm birds in Akure South LGA, Ondo State, Nigeria
Authors: Olonisakin Akinbode Ayekooto , Olusi Titus Adeniyi 
Published: October 2021

Abstract
Background
Cryptosporidium spp. is an intracellular zoonotic protozoan parasite that causes cryptosporidiosis, a diarrhoeal disease of humans and domestic animals. Transmission of Cryptosporidiosis to humans and other animals is by ingestion of oocysts of the parasite and as low as ten oocysts can cause clinical infections in otherwise healthy persons. The aim of this study is to investigate the prevalence of Cryptosporidiosis and compare the rate of infection between free range bird and poultry bird reared in Akure South LGA, Ondo State, Nigeria.

Result
The overall prevalence of Cryptosporidium reported in this study was 11.9%. Free-range birds show a higher prevalence rate 13.2% of Cryptosporidium oocysts than 10.9% in poultry birds. Aule recorded the highest prevalence of Cryptosporidium oocysts infection (16.1%) followed by Ipinsa (12.2%), Onigari (10%), and FUTA (8.1%). The highest prevalence 15.9% was recorded in broilers, while turkey showed no infection (0%) by Cryptosporidium. Semi-intensive system of farming was showed to be more susceptible to Cryptosporidium oocysts infection at 13.3% followed by the 12.6%, 10.3% in deep litter and battery cage. The female birds recorded higher Cryptosporidium oocysts infection (12.2%) than the male (11.6%).

Conclusion
The study established the presence of Cryptosporidium oocysts infection among studied birds in Akure South LG of Ondo State, Nigeria.
Efficient chemical stabilization of tannery wastewater pollutants in a single step process: Geopolymerization
Authors: Boldrini Giacomo , Sgarlata Caterina , Lancellotti Isabella , Barbieri Luisa , Giorgetti Marco , Ciabocco Michela , Zamponi Silvia , Berrettoni Mario , Leonelli Cristina 
Published: October 2021

AbstractThe treatment of tannery wastewaters is a complex task due to the complexity of the waste: a mixture of several pollutants, both anionic and cationic as well as organic macromolecules which are very hard to treat for disposal all together. Geopolymers are a class of inorganic binders obtained by alkali activation of aluminosilicate powders at room temperature. Such activation process leads to a cement like matrix that drastically decreases mobility of several components via entrapment. This process taking place in the matrix can be hypothesized to be the in-situ formation of zeolite structures. In this work we use a metakaolin based geopolymer to tackle the problem directly in an actual industrial environment. To obtain a geopolymer, the metakaolin was mixed with 10 wt% of wastewater added with sodium hydroxide and sodium silicate as activating solutions. This process allowed a rapid consolidation at room temperature, the average compressive strength was between 14 and 43 MPa. Leaching tests performed at different aging times confirm a high immobilization efficiency close to 100%. In particular, only the 0.008 and 2.31% of Chromium and Chlorides respectively are released in the leaching test after 7 months of aging.
Nb2O5 monolith as an efficient and reusable catalyst for textile wastewater treatment
Authors: Troncoso Franco David , Tonetto Gabriela Marta 
Published: October 2021

AbstractIn this work, the degradation of methylene blue (MB) in aqueous solution by a heterogeneous Fenton-like process was studied. Nb2O5 supported on an anodized aluminum monolith was used as catalyst. The Nb2O5 coating generated during the catalyst synthesis was uniform and porous, with a thickness of 7 μm. Response surface methodology was used to study the relationship between process variables and MB degradation and chemical oxygen demand (COD) reduction. The optimal operating conditions were: initial MB concentration of 45 mg L− 1, initial H2O2 concentration in the reaction medium of 40 mM, catalyst loading of 7.85 $$ {\mathrm{kg}}_{{\mathrm{Nb}}_2{\mathrm{O}}_5} $$

kg

Nb
2

O
5

m− 3, and pH 4, reaching a MB degradation of 98% and a COD reduction of 79% at 150 min. The high activity of the Nb2O5 monolith catalyst remained constant after 10 catalytic tests.
Targeting mitochondrial biogenesis: a potential approach for preventing and controlling diabetes
Authors: Singh Ritika , Mohapatra Lucy , Tripathi Alok Shiomurthi 
Published: October 2021

Abstract
Background
Diabetes mellitus is a lingering hyperglycemic ailment resulting in several life-threatening difficulties. Enduring hyperglycemia often persuades the buildup of reactive oxygen species that are the significant pathological makers of diabetic complications. The mitochondrial dysfunction, with mitochondrial damage and too much production of reactive oxygen species, have been proposed to be convoluted in the progress of insulin resistance. Numerous studies advocate that agents that enhance the mitochondrial number and/or decrease their dysfunction, could be greatly helpful in management of diabetes and its complications.

Main body
Mitochondrial biogenesis is an extremely delimited procedure arbitrated by numerous transcription influences, in which mitochondrial fusion and fission happen in synchronization in a standard vigorous cell. But this synchronization is greatly disturbed in diabetic condition designated by modification in the working of several important transcription factors regulating the expressions of different genes. Numerous preclinical and clinical investigations have suggested that, the compromised functions of mitochondria play a significant protagonist in development of pancreatic β-cell dysfunction, skeletal muscle insulin resistance and several diabetic complications. However, there are several phytoconstituents performing through numerous alleyways, either unswervingly by motivating biogenesis or indirectly by constraining or averting dysfunction and producing a beneficial effect on overall function of the mitochondria.

Conclusion
This review describes standard mitochondrial physiology and anomalous modifications that transpire in answer to persistent hyperglycemia in diabetes condition. It also discusses about the different phytoconstituents that can affect the biogenesis pathways of mitochondria and thus can be used in the treatment and prevention of diabetes.
Prevalence of obstructive sleep apnea among patients with chronic obstructive pulmonary disease
Authors: Mohammad Osama Ibrahim , Elgazzar Ahmed Gouda , Mahfouz Shymaa Mohammad , Elnaggar Marwa Elsayed 
Published: October 2021

Abstract
Background
The conjunction of chronic obstructive pulmonary disease (COPD) and obstructive sleep apnea (OSA) is known as overlap syndrome (OS). The coexistence of these diseases has cardiovascular morbidity and mortality. The aim of this study is to assess the prevalence of OSA in COPD patients. One hundred COPD patients (obese and non-obese) performed sleep questionnaires and polysomnograms.

Results
OSA prevalence in COPD was 50% and it increases with increasing disease severity (P < 0.001). The highest prevalence of OSA was found in obese patients with severe COPD; 90.5% of these patients have OSA. In the OSA group, obese patients were found to have significantly higher STOP-Bang Questionnaire (SBQ), Epworth Sleep Scale (ESS), modified medical research council (mMRC) dyspnea scale, apnea-hypopnea index (AHI), respiratory disturbance index (RDI), and oxygen desaturation index (ODI). Both obese and non-obese COPD patients showed significant positive correlations between AHI and smoking index (SI), SBQ, ESS, mMRC, ODI, and neck circumference (NC).

Conclusions
From this study, it can be concluded that moderate and severe COPD patients had a higher diagnosis of sleep-disordered breathing. Also, obese-COPD patients are more susceptible to develop OSA.

Trial registration
Name of the registry: Benha University Protocol Record Benha U123, Obstructive Sleep Apnea Prevalence in Patients With Chronic Obstructive Pulmonary Diseases. Trial registration number:
NCT04903639. Date of registry: 5/22/2021 (retrospective study).
Cancer Hospital Stockpiles: Strategizing for an Efficient and Sufficient Inventory List of Essential Items
Authors: Khawaja Shehryar Nasir , Qadri Hussain Ahmed , Yusuf Muhammed Aasim 
Published: October 2021

PURPOSE The COVID-19 pandemic has affected health care systems worldwide, resulting in critical shortages of essential items and materials. The available guidelines are of little use for cancer hospitals in low-income and low-middle–income countries. They have been designed for community hospitals serving in a centralized health care network. This study aimed to draft and field test a framework to establish a list of essential supplies that should be stockpiled for subsequent waves of the COVID-19 virus by a tertiary care cancer hospital in a low-middle–income country. MATERIALS AND METHODS A model was formulated using the consumption trends during the peak month of the first wave of COVID-19 infection to compile a list of essential materials and supplies. Furthermore, costing analyses were conducted to determine the financial benefits of stockpiling. RESULTS A proposed list of items to stockpile, including personal protective equipment, radiology supplies, laboratory reagents, medication, and oxygen, was shared with the hospital administration. However, the hospital administration only accepted the proposals for stockpiling personal protective equipment and oxygen. CONCLUSION This paper provides a framework and strategies that cancer hospitals and health care systems can modify and use as per individual, institutional requirements and specifications for stockpiling essential items during the COVID-19 or other similar pandemics.
CDC50A is required for aminophospholipid transport and cell fusion in mouse C2C12 myoblasts
Authors: Grifell-Junyent Marta , Baum Julia F. , Välimets Silja , Herrmann Andreas , Paulusma Coen C. , López-Marqués Rosa L. , Günther Pomorski Thomas 
Published: October 2021

ABSTRACT
Myoblast fusion is essential for the formation of multinucleated muscle fibers and is promoted by transient changes in the plasma membrane lipid distribution. However, little is known about the lipid transporters regulating these dynamic changes. Here, we show that proliferating myoblasts exhibit an aminophospholipid flippase activity that is downregulated during differentiation. Deletion of the P4-ATPase flippase subunit CDC50A (also known as TMEM30A) results in loss of the aminophospholipid flippase activity and compromises actin remodeling, RAC1 GTPase membrane targeting and cell fusion. In contrast, deletion of the P4-ATPase ATP11A affects aminophospholipid uptake without having a strong impact on cell fusion. Our results demonstrate that myoblast fusion depends on CDC50A and may involve multiple CDC50A-dependent P4-ATPases that help to regulate actin remodeling.
This article has an associated First Person interview with the first author of the paper.
Impact of Mitomycin-C-Induced Neutropenia after Hyperthermic Intraperitoneal Chemotherapy with Cytoreductive Surgery in Colorectal Cancer Patients with Peritoneal Carcinomatosis
Authors: Lee Suk Jun , Jeon Youngbae , Lee Hae Won , Kang Jeonghyun , Baik Seung Hyuk , Park Eun Jung 
Published: October 2021

Abstract
Background
Mitomycin-C (MMC) is the most commonly used chemotherapeutic agent for hyperthermic intraperitoneal chemotherapy (HIPEC) after cytoreductive surgery (CRS). However, MMC has a side effect of myelosuppression. This study aimed to evaluate the clinical manifestations and impact of MMC-induced neutropenia after CRS and HIPEC in colorectal cancer patients.

Methods
A total of 124 colorectal cancer patients who underwent CRS with HIPEC between March 2015 and January 2019 were evaluated. Patients with malignancies of non-colorectal origin, hospital stay longer than 60 days, peritoneal cancer index > 30, and complete cytoreduction score > 2 were excluded. MMC 35 mg/m2 was administered for 90 min at 41–43 °C. The patients were divided into three groups: no neutropenia, mild neutropenia (grade 1–2), and severe neutropenia (grade 3–4).

Results
In total, mild and severe neutropenia occurred in 30 (24.2%) and 48 (38.7%) patients, respectively. Age and body surface area were significantly different among the neutropenia groups. Severe neutropenia developed significantly earlier than mild neutropenia (6.9 days vs. 10.4 days, p < 0.001) and also lasted significantly longer (4.6 days vs. 2.5 days, p = 0.005). The rate of major postoperative complications was significantly higher in the severe neutropenia group than in the no and mild neutropenia groups (8.3% vs. 6.7% vs. 6.5%, p = 0.015)

Conclusions
Severe neutropenia starts earlier and lasts longer than mild neutropenia after CRS and HIPEC using an MMC triple method. The higher rate of major postoperative complications in patients with severe neutropenia highlights the importance of postoperative management during the neutropenia period.
Digital taxation to promote frugal innovation in institutions of higher learning: a three-decade systematic literature review
Authors: Kwan Wei Ling , Dorasamy Magiswary , Bin Ahmad Abdul Aziz , Jayabalan Jayamalathi , Kumar Pradeep , Subermaniam Lingeswaran 
Published: October 2021

Background: In the era of the Fourth Industrial Revolution (IR 4.0), digital taxation emerged as a tool for accelerating the economic growth of a nation. While Industry 4.0 focuses on enabling real-time decision-making with sophisticated technology to enhance productivity, digital taxation can serve as an important tool for improving business sustainability. Institutions of higher learning (IHL), which aim to design an IR 4.0 educational ecosystem, can embrace digital taxation, as they face various challenges with different resources. The literature indicates that frugal innovation through digital taxation in institutions of higher learning, can solve emerging resource challenges. Method: We present a systematic review of studies on digital taxation to promote frugal innovation published in the past three decades (1991 to 2021). We obtained a total of 21 papers from a ‘digital taxation’ keyword search, 10 of which were related to digital taxation. However, the 10 papers were not related to frugal innovation. Result: We present two major findings. Firstly, research on digital taxation for frugal innovation is scant. Secondly, challenges exist in digital taxation implementation, which requires further attention. Conclusion: We conclude this review with a recommendation for the conceptual framework, to highlight potential research warranting the attention of the research community.
Project PaThWay: protocol for a school-based health promotion intervention for prevention of non-communicable diseases (NCDs) behavioral risk factors
Authors: Bassi Shalini , Bahl Deepika , Shah Vinod Gajanan , Kandasamy Arun , Harrell Melissa Blythe , Sharma Shreela V , Arora Monika 
Published: October 2021

Background: Prevention of non-communicable diseases (NCDs) and their behavioral risk factors (tobacco use, unhealthy diet, physical inactivity, harmful use of alcohol) among children and adolescents have garnered paramount importance under the Sustainable Development Goals. Methods: Project PaThWay is a school-based, two years, multi-component intervention to prevent key behavioral NCD risk factors among school-going children (classes 6-8th; 10-14 years) from private and public schools across two Indian cities (Pune and Bengaluru). We assessed the baseline knowledge, attitude, and behavior related to NCD risk factors (unhealthy diet, physical inactivity, and tobacco use) of the participating students through a survey. The intervention was developed and designed for implementation by the trained teachers and similar-age peers, as facilitators. The teachers and peer leaders were trained through organising school-level orientation workshops for implementation of intervention activities, after baseline assessment. Year 1 of the intervention focuses on the knowledge and learnings and year 2 on enhancing the life-skills (leadership, communication, refusal, health advocacy, etc.). Regular monitoring visits by the project team to ensure intervention activities are being carried out as planned and providing continuous support. The end line evaluation will be done after the completion of two years’ intervention to evaluate the effectiveness of the Project PaThWay intervention. Outcome measures will include improved knowledge, positive attitude, improve behaviors related to diet, physical activity, and tobacco use, and enhanced skills in handling NCD risk factors. A process evaluation will explore several aspects of Project PaTHWay intervention (fidelity, dosage, reach, adaptations), social validity (acceptability, feasibility, utility). Conclusion: Project PaTHWay, having a multiple-component intervention, may offer the best chance for success, as it addresses multiple risk factors using multi-pronged strategies. The agents of intervention implementation are trained teachers and similar-age student peer leaders (as facilitators), one of the successful and effective approaches in school-based interventions globally and in India.