bluuebunny/crossref_metadata_2025_split huggingface dataset:
Hypoxia-Related Gene FUT11 Promotes Pancreatic Cancer Progression by Maintaining the Stability of PDK1
Authors: Cao Wenpeng , Zeng Zhirui , Pan Runsang , Wu Hao , Zhang Xiangyan , Chen Hui , Nie Yingjie , Yu Zijiang , Lei Shan 
Published: June 2021

BackgroundHypoxia is associated with the development of pancreatic cancer (PC). However, genes associated with hypoxia response and their regulatory mechanism in PC cells were unclear. The current study aims to investigate the role of the hypoxia associated gene fucosyltransferase 11 (FUT11) in the progression of PC.MethodsIn the preliminary study, bioinformatics analysis predicted FUT11 as a key hypoxia associated gene in PC. The expression of FUT11 in PC was evaluated using quantitative real-time PCR (qRT-PCR), Western blot and immunohistochemistry. The effects of FUT11 on PC cells proliferation and migration under normoxia and hypoxia were evaluated using Cell Counting Kit 8, 5-ethynyl-2’-deoxyuridine (EDU) assay, colony formation assay and transwell assay. The effects of FUT11 in vivo was examined in mouse tumor models of liver metastasis and subcutaneous xenograft. Furthermore, Western blot, luciferase assay and immunoprecipitation were performed to explore the regulatory relationship among FUT11, hypoxia-inducible factor 1α (HIF1α) and pyruvate dehydrogenase kinase 1 (PDK1) in PC.ResultsFUT11 was markedly increased of PC cells with hypoxia, upregulated in the PC clinical tissues, and predicted a poor outcome of PC patients. Inhibition of FUT11 reduced PC cell growth and migratory ability of PC cells under normoxia and hypoxia conditions in vitro, and growth and tumor cell metastasis in vivo. FUT11 bound to PDK1 and regulated the expression PDK1 under normoxia and hypoxia. FUT11 interacted with PDK1 and decreased the ubiquitination of PDK1, lead to the activation of AKT/mTOR signaling pathway. FUT11 knockdown significantly increased the degradation of PDK1 under hypoxia, while treatment with MG132 can relieve the degradation of PDK1 induced by FUT11 knockdown. Overexpression of PDK1 in PC cells under hypoxia conditions reversed the suppressive impacts of FUT11 knockdown on PC cell growth and migration. In addition, HIF1α bound to the promoter of FUT11 and increased its expression, as well as co-expressed with FUT11 in PC tissues. Furthermore, overexpression of FUT11 partially rescued the suppressive effects of HIF1α knockdown on PC cell growth and migration in hypoxia condition.ConclusionOur data implicate that hypoxia-induced FUT11 contributes to proliferation and metastasis of PC by maintaining the stability of PDK1, thus mediating activation of AKT/mTOR signaling pathway, and suggest that FUT11 could be a novel and effective target for the treatment of pancreatic cancer.
Development and Longitudinal Analysis of Plan-Based Streamlined Quality Assurance on Multiple Positioning Guidance Systems With Single Phantom Setup
Authors: Zhou Shun , Li Junyu , Du Yi , Yu Songmao , Wang Meijiao , Wu Hao , Yue Haizhen 
Published: June 2021

PurposeThis study was to propose and validate an efficient and streamlined quality assurance (QA) method with a single phantom setup to check performances of patient positioning guidance systems including six-degree-of-freedom (6DoF) couch, X-ray modalities (kV–kV, MV–MV and CBCT), optical surface imaging system (AlignRT), lasers and optical distance indicator (ODI).Methods and MaterialsThe QA method was based on a pseudo-patient treatment plan using the AlignRT cube phantom. The cube was first randomly set up on the couch, and the initial position offsets were acquired by AlignRT and CBCT. The cube was restored to its reference position by 6DoF couch shift, during which the couch motion accuracy and tracking performances of AlignRT and CBCT were derived. After that, the residual offsets were acquired by kV–kV, MV–MV and AlignRT to derive the isocenter discrepancies. Finally, the laser alignment and ODI values were visually inspected. The QA procedure had been internally approved as a standard weekly QA test, and the results over 50 weeks were longitudinally analyzed for clinical validation.ResultsThe 6DoF couch motion errors as well as the tracking errors of AlignRT were sub-millimeter and sub-degree, and no deviation over 1 mm or 1 deg was identified. The ROI mode of isocenter (ISO) in AlignRT exhibited more consistent results than the centroid (CEN). While the isocenter discrepancy between CBCT and kV–kV was negligible, the maximal discrepancies between CBCT and MV–MV were 0.4 mm in LNG and 0.3 deg in PITCH. The isocenter discrepancies between CBCT and AlignRT were &lt;0.5 mm in translation and &lt;0.3 deg in rotation. For AlignRT, the isocenter discrepancies between the DICOM and SGRT references were about 0.6 mm in VRT, 0.5 mm in LNG and 0.2 deg in PITCH. As the therapists became familiar with the workflow, the average time to complete the whole procedure was around 23 min.ConclusionsThe streamlined QA exhibits desirable practicality as an efficient multipurpose performance check on positioning guidance systems. The stability, tracking performance and isocenter congruence of the positioning guidance systems have been fully validated for all clinical image guidance RT application, even SRS/SBRT, which requires the strictest tolerance.
Clinical Study of Paliperidone Palmitate Long-Acting Injection Combined With Electroacupuncture in the Treatment of Methamphetamine Addicts
Authors: Chen Yu , Li Mingchao , Ji Qiuming , Su Zou , Yang Ziyu , Xu Yin , Chen Qian , Liao Dan , Zeng Jihua , Yang Yuhong 
Published: June 2021

Objective: To explore the clinical efficacy of paliperidone palmitate long-acting injection combined with electroacupuncture in the treatment of methamphetamine addicts.Methods: This study focused on methamphetamine addicts who were admitted to our hospital from January 2020 to December 2020 as the main research object, with a total of 89 cases. The patients were divided into a control group of 45 cases and a study group of 44 cases according to the treatment method. The control group was treated with electroacupuncture, and the study group was treated with paliperidone palmitate long-acting injection on the basis of electroacupuncture in the control group. After 6 months of continuous treatment, the treatment effect of methamphetamine withdrawal symptom score before and after treatment was used; Hamilton Anxiety Scale score and Hamilton Depression Scale were used to compare the anxiety and depression situation of the two groups; the brain wave α and θ wave situation of the two groups were compared.Result: The results showed that there was no significant difference in the scores of Ma withdrawal symptoms, Hamilton Anxiety and Hamilton Depression between the two groups before treatment (p &lt; 0.05); after 3 and 6 months of treatment, the scores of Ma withdrawal symptoms, Hamilton Anxiety and Hamilton Depression in the study group were significantly lower than those in the control group and the difference was statistically significant (p &lt; 0.05); 6 months after the completion of the treatment, the α wave amplitude and Fourier transformed α brain wave (FFTα) in the study group were significantly higher than those in the control group, and the difference was statistically significant (p &lt; 0.05).Conclusion: Paliperidone palmitate long-acting injection combined with electroacupuncture is better than electroacupuncture alone in the treatment of methamphetamine addicts, and can significantly improve anxiety, depression and brain waves, thereby preventing addicts from relapse.
Effects of Glucose Oxidase Supplementation on the Growth Performance, Antioxidative and Inflammatory Status, Gut Function, and Microbiota Composition of Broilers Fed Moldy Corn
Authors: Qu Wenhui , Liu Jiaguo 
Published: June 2021

BackgroundGlucose oxidase is widely used as a livestock feed additive owing to its beneficial effects on growth performance and antioxidant activity. However, little is known about the effects of the enzyme on intestinal health.MethodsTo investigate the effects of glucose oxidase supplementation on the growth performance, intestinal function, and microbiota composition of broilers fed moldy corn, newly hatched Arbor Acres broilers were each randomly assigned to one of four groups, which were fed a basal diet (CON), a contaminated diet (10% moldy corn) (MC), a basal diet supplemented with 0.01% glucose oxidase (GOD), or a contaminated diet supplemented with 0.01% glucose oxidase (MCG).ResultsWe found that the average weight gain (ADG) of the MC group was significantly lower than those of the CON and GOD groups, and there were no significant differences in ADG between the MCG group and the CON and GOD groups. Intestinal morphology results revealed irregularly arranged villi and microvilli in the ilea from the MC group, whereas those from the other three groups were aligned regularly. Tight-junction protein analysis showed that both ZO-1 expression and claudin-4 expression in the MC group were significantly lower than those in the other groups. Inflammation cytokines analysis showed lower serum concentration of interleukin-10, as well as its mRNA expression in the ileum of the MC group, when compared with those of the other groups. Additionally, we observed lower glutathione peroxidase and total superoxide dismutase activity and higher malonaldehyde concentration in the MC group than those in the MCG group. The α and β diversity of microbiota profiling indicated that the cecal microbiota in the MC group differed from those in the other three groups.ConclusionThe results indicated that glucose oxidase supplementation was able to prevent the adverse effects from mycotoxin exposure on growth performance, antioxidant activity, inflammatory response, intestinal function, and microbiota composition in broilers. We suggested that glucose oxidase supplementation can be used in broilers to mitigate the adverse effects of moldy feed, and its benefits are due to its effect on intestinal microbiota composition.
Minor Physical Anomalies in Bipolar Disorder—A Meta-Analysis
Authors: Varga Eszter , Hajnal András , Soós Alexandra , Hegyi Péter , Kovács Dóra , Farkas Nelli , Szebényi Júlia , Mikó Alexandra , Tényi Tamás , Herold Róbert 
Published: June 2021

Introduction: Minor physical anomalies (MPAs) may reflect basic neurobiological features underlying bipolar disorders (BPD), as they are sensitive physical indicators of morphogenetic failure of the brain. Despite several researches about the presence of MPAs in BPD, the results are still controversial.Objectives: The aim of the present meta-analysis was to assess the standardized weighted mean effect sizes of MPAs in BPD and to examine if MPAs may be found predominantly in the head and/or facial regions in BPD patients compared to controls (HC).Methods: Four studies, involving 155 patients with BPD, and 187 HC, were involved in the analysis after searching the literature. For the investigation of MPAs in the peripheral (MPA-P) and in the head and facial regions (MPA-CF), two studies involving 121 BPD patients, and 133 HC passed the inclusion criteria.Results: The number of the MPAs in the BPD group was significantly higher compared to HC. Another important finding of the present study is that BPD patients' MPA-P scores do not significantly differ from those of the HC. In contrast, BPD patients' MPA-CF scores were found to be significantly higher compared to HC subjects. It is important to note that there was a low number of eligible publications included, which caused higher heterogeneity.Conclusions: Low quality of evidence suggests that MPAs are more common in patients with BPD than in HC and the higher rate of MPAs is found predominantly in the head and facial regions.
Staff SARS-CoV-2 Seroprevalence and Mental Health as Key Factors in University Response to COVID-19 Pandemic
Authors: Lopes David G. , Henriques Ana Rita , Santos-Dias Margarida , Nunes-da-Silva Catarina , Gonçalves Juliana , de Sousa Rute D. , Abdulghani Saba , Eletério Jair , Braga Sofia Jacinto , Soares Helena , Branco Jaime C. , Canhão Helena , Rodrigues Ana M. 
Published: June 2021

Background: In response to rapid global spread of the newly emerged coronavirus disease 2019 (COVID-19), universities transitioned to online learning and telework to decrease risks of inter-person contact. To help administrators respond to the COVID-19 pandemic and better understand its impacts, we surveyed SARS-CoV-2 seroprevalence among NOVA University employees and assessed community mental health.Methods: Data were collected from voluntary participants at six NOVA University locations, in the Lisbon metropolitan area, from June 15–30, 2020. All subjects provided written informed consent. Of 1,627 recruited participants (mean age 42.0 ± 12.3 years), 1,624 were tested. Prior to blood collection, participants completed a questionnaire that assessed: COVID-19 symptoms during the previous 14 days, chronic non-communicable diseases, chronic medication, anxiety, and depression symptoms. SARS-CoV-2 serology tests were then performed, and results communicated approximately 4 days after blood draw. Participants with positive serology tests were contacted to assess COVID-19 symptoms since February.Results: Estimated prevalence of SARS-CoV-2 IgG antibodies was 3.1% (n = 50), of which 43.5% reported symptoms in the previous 4 months. The Medical School had the highest seroprevalence (6.2%). Participants reported having at least one chronic disease (63.7%), depression-like symptoms (2.1%), and anxiety symptoms (8.1%). Rates of depression and anxiety symptoms were significantly higher in women, with sleep hours and occasional alcohol consumption negatively associated with depression. Male gender, older age, and sleep hours negatively associated with anxiety symptoms. School of employment and presence of comorbidities positively associated with anxiety.Conclusion: By measuring seroprevalence of SARS-CoV-2 antibodies among NOVA employees and assessing subjects' mental health, we aim to help administrators at European public universities in urban areas, such as Lisbon, Portugal, better understand the needs of their communities. This study resulted in implementation of a stricter contingency plan in the Medical School, while other schools continued to follow Government mitigation guidelines. These findings may also guide the development of tailored strategies to ensure physical and mental health of the academic community during this pandemic crisis. We conclude that, together with COVID-19 contingency plans, psychological support services and facilities to help people effectively face pandemic-associated challenges and minimise anxiety and depression should be implemented.
A Comparison of the Simulations and Observations for a Nearby Spiral Arm
Authors: Piecka Martin , Paunzen Ernst 
Published: June 2021

The analysis is focused on the ability of galactic open clusters to trace the spiral arms, based on the recent data releases from Gaia. For this, a simple 1D description of the motion of spiral arms and clusters is introduced. As next step, results are verified using a widely accepted kinematic model of the motion in spiral galaxies. As expected, both approaches show that open clusters older than about 100 Myr are bad tracers of spiral arms. The younger clusters (ideally &lt; 30 Myr) should be used instead. This agrees with the most recent observational evidence. The latest maps of the diffuse interstellar bands are compared with the spiral structure of the Milky Way and the Antennae Galaxies. The idea of these bands being useful for studying a galactic structure cannot be supported based on the current data.
Thrombolytic Therapy During ex-vivo Normothermic Machine Perfusion of Human Livers Reduces Peribiliary Vascular Plexus Injury
Authors: Haque Omar , Raigani Siavash , Rosales Ivy , Carroll Cailah , Coe Taylor M. , Baptista Sofia , Yeh Heidi , Uygun Korkut , Delmonico Francis L. , Markmann James F. 
Published: June 2021

Background: A major limitation in expanding the use of donation after circulatory death (DCD) livers in transplantation is the increased risk of graft failure secondary to ischemic cholangiopathy. Warm ischemia causes thrombosis and injury to the peribiliary vascular plexus (PVP), which is supplied by branches of the hepatic artery, causing higher rates of biliary complications in DCD allografts.Aims/Objectives: We aimed to recondition discarded DCD livers with tissue plasminogen activator (tPA) while on normothermic machine perfusion (NMP) to improve PVP blood flow and reduce biliary injury.Methods: Five discarded DCD human livers underwent 12 h of NMP. Plasminogen was circulated in the base perfusate prior to initiation of perfusion and 1 mg/kg of tPA was administered through the hepatic artery at T = 0.5 h. Two livers were split prior to perfusion (S1, S2), with tPA administered in one lobe, while the other served as a control. The remaining three whole livers (W1-W3) were compared to seven DCD control liver perfusions (C1-C7) with similar hepatocellular and biliary viability criteria. D-dimer levels were measured at T = 1 h to verify efficacy of tPA. Lactate, total bile production, bile pH, and difference in biliary injury scores before and after perfusion were compared between tPA and non-tPA groups using unpaired, Mann-Whitney tests.Results: Average weight-adjusted D-dimer levels were higher in tPA livers in the split and whole-liver model, verifying drug function. There were no differences in perfusion hepatic artery resistance, portal vein resistance, and arterial lactate between tPA livers and non-tPA livers in both the split and whole-liver model. However, when comparing biliary injury between hepatocellular and biliary non-viable whole livers, tPA livers had significantly lower PVP injury scores (0.67 vs. 2.0) and mural stroma (MS) injury scores (1.3 vs. 2.7).Conclusion: This study demonstrates that administration of tPA into DCD livers during NMP can reduce PVP and MS injury. Further studies are necessary to assess the effect of tPA administration on long term biliary complications.
AKTIVITAS ANTIOKSIDAN EKSTRAK TERIPANG (H.atra) DENGAN METODE DPPH (1,1-diphenyl-2-picrylhydrazyl)
Authors: Bawole Avinda Shania Wiane , Wewengkang Defny S. , Antasionasti Irma 
Published: June 2021

ABSTRACTUtilization of marine life is not just consumptive but can be used as raw material for medicines, one of which is sea cucumber. Sea cucumbers (H.atra) are marine invertebrates belonging to the Echinoderms phylum which have been studied containing antioxidants. Therefore, a study was conducted to determine the antioxidant activity of sea cucumber (H.atra) extract taken from the waters of Manado Bay using the DPPH (1,1-diphenyl-2-picrylhydrazyl) method. Sea cucumber samples were extracted using the maceration method. The ethanol extract of sea cucumber showed high antioxidant activity at a concentration of 8 μg / ml with 77.83% inhibition percentage. Keywords: H.atra, Antioxidant, DPPH (1,1-diphenyl-2-picrylhydrazyl). ABSTRAKPemanfaatan biota laut bukan hanya sekedar konsumtif saja tapi dapat berpotensi sebagai bahan baku obat-obatan salah satunya teripang. Teripang (H.atra) adalah hewan invertebrata laut yang termasuk ke dalam filum Echinodermata yang pernah diteliti mengandung antioksidan. Oleh karena itu, dilakukan penelitian untuk mengatehui aktivitas antioksidan dari ekstrak teripang (H.atra) yang diambil dari perairan Teluk Manado dengan menggunakan metode DPPH (1,1-diphenyl-2-picrylhydrazyl). Sampel teripang diekstraksi menggunakan metode maserasi. Ekstrak etanol teripang menunjukkan aktivitas antioksidan yang tinggi pada konsentrasi 8 μg/ml dengan persen inhibisi 77,83%. Kata kunci: H.atra, Antioksidan, DPPH (1,1-diphenyl-2-picrylhydrazyl)
Analisis Kualitas Website Integrated Lab Journal Menggunakan Webqual Dan Importance Performance Analysis
Authors: Mulyanto Agus 
Published: June 2021

Website Integrated Lab Journal merupakan salah satu website e-journal yang diterbitkan oleh Universitas Islam Negeri Sunan Kalijaga Yogyakarta sebagai media untuk makalah ilmiah tentang pendidikan laboratorium. Instrumen penelitian ini menggunakan metode webqual yang terdiri dari 3 variabel yaitu usability (kegunaan), information quality (kualitas informasi), dan service interaction quality (kualitas interaksi layanan) dan 23 indikator. Analisis Importance Performance Analysis (IPA) dalam penelitian ini mengidentifikasi indikator-indikator yang perlu dipertahankan atau diperbaiki berdasarkan persepsi dan harapan pengguna. Pengujian webqual melibatkan 100 orang akademisi dari berbagai universitas di Indonesia. Nilai kesenjangan paling tinggi terdapat pada variabel usability (kegunaan) yaitu u5 mengenai tampilan website yang menarik dengan nilai kesenjangan -0.830. Hasil analisis IPA menunjukan indikator yang memerlukan perbaikan yaitu u3, i4, i6, dan s3. Indikator-indikator tersebut berhubungan dengan kemudahan navigasi website, informasi yang relevan dan sesuai kebutuhan pengguna, informasi yang rinci dan lengkap, dan rasa aman ketika memberikan informasi pribadi. Indikator yang dinilai baik oleh pengguna terdapat dalam kuadran I, yaitu u1, u4, u7, u10, i1, i2, i5, s2, dan s6. Indikator-indikator tersebut berhubungan dengan kemudahan pengoperasian, kemudahan penggunaan, pelaksaan fungsi yang baik, pencarian yang efektif, keakuratan informasi, informasi dapat dipercaya, informasi mudah dimengerti, kepercayaan publikasi, dan keyakinan proses editorial.

Kata kunci : E-journal, Integrated Lab Journal, Webqual, Importance Performance Analysis (IPA)
Mengelola Taman Budaya Di Lapangan Dewata Dengan Protokol Kesehatan Di Era New Normal
Authors: Melinda Tina , Kodrat David Sukardi , Ritunga Imelda 
Published: June 2021

Desa Wage kecamatan Taman kabupaten Sidoarjo adalah tempat strategis, karena dikelilingi oleh perusahaan besar. Perekonomian desa Wage mayoritas dari sektor perniagaan seperti menjual kuliner. Saat pandemic Covid-19 agar dapat tetap melakukan kegiatan perekonomian maka masyarakat harus betul-betul menjalankan Pola Hidup sehat. Untuk itu Universitas Ciputra bekerja sama dengan BUMDes Wage Bersinar melaksanakan kegiatan sebagai berikut: pertama menjadikan pengurus Bumdes sebagai role model menjalankan protokol Kesehatan dan menjadikan mayarakat desa Wage sadar akan pentingnya pola hidup sehat agar tetap dapat menjalankan perekonomian. Dalam mengelola taman budaya, maka harus menggunakan protokol Kesehatan, mengingat taman ini adalah multi fungsi selain sebagai sentra kuliner juga sebagai wadah kegiatan masyarakat seperti berolah raga dan pertemuan lain. Metode yang digunakan adalah (1) sosialisasi untuk memberikan pemahaman kepada masyarakat akan pentingnya 3M., (2) pelatihan dan demonstrasi mengenai cara mencuci tangan yang benar, (3) stimulan empowering dengan memberikan spanduk, poster, masker dan tangki tempat cuci tangan, dan (4) asset based community development dengan memperkuat kapasitas masyarakat mengidentifikasi prioritas, peluang untuk mendorong dan mempertahankan perubahan lingkungan yang positif. Keberhasilan gerakan mengelola Taman Budaya Dewata dengan protokol Kesehatan dan aman digunakan untuk menunjang kegiatan desa dan menjalankan ekonomi setempat, serta dapat menjadi contoh bagi lokasi lain yang memiliki sarana yang sama