bluuebunny/crossref_metadata_2025_split huggingface dataset:
Purification Efficiency of Eight Aquatic Plant Species in an Artificial Floating Island System in Relation to Extracellular Enzyme Activity and Microbial Community
Authors: Chen Qingfeng , Feng You , Li Jinye , Li Qing , Liu Ting 
Published: April 2021

Abstract
An artificial floating island is an ecological restoration technology that aims to create sustainable ecosystems and improve biodiversity. Aquatic plants play an important role in wastewater purification. The floating island system exploits the combination of aquatic plants, microorganisms, and extracellular enzymes to purify wastewater. We investigated the purification efficiency of eight aquatic plant species (Ceratophyllum demersum, Elodea nuttallii, Eichhornia crassipes, lris pseudacorus, lris sibirica, Myriophyllum verticillatum, Thalia dealbata and Oenanthe javanica) cultured in wastewater. The relationships of plant purification capacity with extracellular enzyme activity and microbial community were analyzed to explore the crucial factors that affect the plant purification capacity and the mechanism of pollutants removal in different plant systems. Three plant species, namely Oenanthe javanica, Thalia dealbata, and lris pseudacorus, were the most effective for purification of ammonium-nitrogen (NH4+-N), total phosphate (TP), and chemical oxygen demand (COD) with maximum efficiencies of 76.09%, 85.87%, and 89.10%, respectively. Urease, alkaline phosphatase (AP), and β-glucosidase activities were significantly and positively correlated with root system development (P < 0.05). Activities of urease and AP were positively correlated with NH4+-N and TP removal, respectively. The magnitude of urease and AP activity was generally consistent with the plant’s capacity to remove NH4+-N and TP. β-Glucosidase activity and COD removal were not significantly correlated. The dominant microbial phylum in each species treatment was Proteobacteria. Alphaproteobacteria and Bacteroidia showed > 1% relative abundance and greater involvement in degradation of pollutants in the experimental system. The results provide a scientific and theoretical basis for improvement of the plant purification efficiency of artificial floating island systems.
Implementation of Efficient Reconfigurable FIR Filter With Control Logic for 5G Applications
Authors: Kalidindi SN Raju , Terlapu Sudheer Kumar , M Vamshi Krishna 
Published: April 2021

Abstract
Filters are used to achieve frequency selectivity on the spectrum of input signal. Due to the stability of FIR filters, they are used in most of the applications. In the conventional FIR filters the frequency band is fixed and can‟t be changed once it is designed. Hence there is a necessity of an FIR filter with auto adjustment of band width. The design of FIR filter requires more number of filter coefficients to get the desired bandwidth specification. This results in a large slice for FPGA implementation. Here it is proposed a state machine to select different FIR filters with the designated set of coefficients. Each FIR filter is having different set of coefficients and based on the frequency of the clock signal the FIR filter is selected. Therefore frequency selectivity can be achieved. The Proposed method is to implement Reconfigurable FIR Filter with control logic for auto adjustment of fre-quency selections to achieve better band width requirements. The filter order is initially selected as 4 and presented the simulation results. The order of the filter(n) increased to 24 for verifying the bandwidth selection. The proposed architecture is compared with the existing architecture with 16bits and 11taps. Simulation results presented are verified using Xilinx ISE design suite 14.7. Total number of 4 input LUTs utilized are 630 for n=24. Power consumed by the overall design is 195mW.
Application of Chemometrics Into Removal of Dyes by NaX&nbsp;Nanozeolites: Simultaneous Model
Authors: Shojaei Siroos , Shojaei Saeed , Band Shahab S. 
Published: April 2021

Abstract
In the present study, the simultaneous removal of malachite green (MG) and auramine-O (AO) dyes from the aqueous solution by NaX nanozeolites in a batch system is investigated. Taguchi method and response surface methodology (RSM) were used to optimize and model dye removal conditions. In order to do so, the effect of various factors (dyes concentration, sonication time, ionic strength, adsorbent dosage, temperature, and pH of the solution) on the amount of dye removal was evaluated by the Taguchi method. Then, the most important factors were chosen and modeled by the RSM method so as to reach the highest percentage of dye removal. The proposed quadratic models to remove both dyes were in good accordance with the actual experimental data. The maximum removal efficiencies of MG and AO dyes in optimal operating conditions were %99.07 and %99.61, respectively. Also, coefficients of determination (R2) for test data were 0.9983 and 0.9988 for MG and AO dyes, respectively. The reusability of NaX nanozeolites was evaluated during the adsorption process of MG and AO. The results showed that the adsorption efficiency decreases very little up to 5 cycles. Moreover, NaX nanozeolites were also applied as adsorbents to remove MG and AO from environmental water samples and more than %98.1 of both dyes were removed from the solution in optimal conditions.
The Effects of Taurine Supplementation on Inflammatory Cytokines and Physical Performance to Simulated Taekwondo-Specific Protocol in Elite Male Athletes: A Double-Blinded, Placebo-Controlled, Crossover Study
Authors: Samandari Ahmad , Sanjideh Fatemeh , Faramarzi Mohammad , Banitalebi Ebrahim , Ghahfarrokhi Majid Mardaniyan 
Published: April 2021

Abstract
Background: The main purpose of this study was to investigate the effects of taurine supplementation as a countermeasure against decrement in athletic performance to enhance metabolic inflammation status, circulating lactate levels, and functional capacity following a simulated taekwondo competition day with the same physiological pressure in elite male taekwondo athletes.Methods: A double-blinded, placebo-controlled, crossover design was utilized in the present trial. A total number of ten healthy, highly-trained, elite male taekwondo athletes (age=20.60±3.5 years) were accordingly recruited to receive taurine (a dose of 15 mg/ body weight, approximately three servings per day) or the same doses of a placebo for 10 days, separated by a 10-day washout period. The simulated taekwondo competition day included five exhaustive single competition sessions using a special time framework of taekwondo test with the same physiological pressure. Five competition taekwondo stations were also done with a time-course order. Results: The results showed significant time-placebo interaction effect for IL-15 levels (0.001). Using post-hoc comparisons, a significant increase was also found between supplemental and placebo occasions 24 h after the simulated taekwondo competition day (0.001). However, no significant changes were observed in IL-8 (0.19), IL-17 (0.808), hs-CRP (0.766), and TNF-α (p=0.970) levels. In addition, there were no significant changes in final heart rate (p=0.413), countermovement jump (p=0.884), and turning kick (p=0.790) following the simulated taekwondo competition day and taurine supplementation.Conclusions: The results of the present trial did not support the ergogenic effect of short-term (10-day) taurine supplementation on performance and inflammation status of elite male athletes.
Determinants of the Uptake of Intermittent Preventive Treatment of Malaria in Pregnancy with Sulphadoxine Pyrimethamine in Sabatia Sub County, Western Kenya
Authors: Mutanyi Joshua Andala , Onguru Daniel O. , Ogolla Sidney O. , Adipo Lawrence B. 
Published: April 2021

Abstract
Background: Malaria in pregnancy remains a major public health problem. Annually, 125.2 million pregnant women worldwide are at risk of malaria infection including 30.3 million and 1 million pregnant women in Sub-Saharan Africa and Kenya respectively. The World Health Organization recommends that pregnant women in malaria endemic areas receive at least three doses of sulphadoxine pyrimethamine for intermittent preventive treatment of malaria in pregnancy (IPTp-SP) for optimal benefit. However, IPTp-SP optimal uptake is undesirably low in Kenya. This study investigated the prevalence of and factors influencing IPTp-SP optimal uptake in Sabatia Sub County, Western Kenya. Understanding the epidemiology of malaria in pregnancy is core for making decisions and setting priorities towards IPTp-SP optimization.Methods: This was a cross-sectional study conducted in Sabatia Sub County. Using a validated semi structured questionnaire, data were obtained from 372 randomly sampled post-delivery women aged 15 – 49 years who had a live birth within one year preceding the study. Women on cotrimoxazole prophylaxis during their pregnancy were excluded. Association between IPTp-SP uptake and independent variables was analysed using Pearson Chi-square and Fisher’s Exact test. Bivariate and multiple binary logistic regression analysed predictors of optimal IPTp-SP uptake.Results: Overall, 99.46 % of the respondents received at least one IPTp-SP dose. The prevalence of optimal IPTp-SP uptake was 79.57% (95% CI 75.47%, 83.67%). After multivariate analysis; gestational age at first antenatal care (ANC) visit (p = 0.04), frequency of ANC visits (p < 0.001), maternal knowledge of IPTp-SP benefits (p < 0.001), maternal knowledge of optimal SP dose (p = 0.03) and administration of sulphadoxine pyrimethamine at ANC clinic (p = 0.03) significantly predicted the optimal uptake of IPTp-SP.Conclusions: Optimal uptake of IPTp-SP is high in the study area. Efforts towards early and more frequent ANC attendance should be enhanced and sustained. Structured and targeted health education should be adopted and health workers should always administer SP drugs or explain to some pregnant women their ineligibility for initial IPTp-SP receipt. Future studies considering large sample drawn from the whole country and health workers’ perspective of the health system delivery factors are recommended.
Near Adult Heights and Predictions of Prospective Adult Heights using Automated and Conventional Greulich-Pyle Bone Age Determinations in children with chronic endocrine diseases
Authors: Choukair Daniela , Hückmann Annette , Mittnacht Janna , Breil Thomas , Schenk Jens Peter , Alrajab Abdulsattar , Uhlmann Lorenz , Bettendorf Markus 
Published: April 2021

Abstract
Calculation of prospective adult heights (PAH) is associated with considerable bone age interrater variability. Therefore, the new PAH method based on automated bone age (BA) determination (BoneXpert™) was compared to the conventional PAH method by Bayley- Pinneau (BP) based on BA determination according to Greulich and Pyle (GP) and to observed near adult heights. Heights and near adult heights were measured in 82 patients (48 females) with chronic endocrinopathies at age of 10.45 ± 2.12 years and at time of transition to adult care (17.98 ± 3.02 years). Further, BA were assessed according to conventional GP - by three experts- and by BoneXpert™. PAH were calculated using conventional BP tables and BoneXpert™. The conventional and the automated BA determinations revealed a mean difference of 0.25 ± 0.72 years (p = 0.0027). The automated PAH by BoneXpert™ were 156.96 ± 0.86 cm in females and 171.75 ± 1.6 cm in males compared to 153.95 ± 1.12 cm in females and 169.31 ± 1.6 cm in males by conventional BP, respectively, and in comparison to near adult heights 156.38 ± 5.84 cm in females and 168.94 ± 8.18 cm in males, respectively. Conclusion: BA ratings and adult height predictions by BoneXpert™ in children with chronic endocrinopathies abolish rater dependent variability and enhance reproducibility of estimates thereby refining care in growth disorders. Conventional methods may outperform automated analyses in specific cases.
Single-cell ATAC and RNA sequencing reveal pre-existing and persistent subpopulations of cells associated with relapse of prostate cancer
Authors: Taavitsainen Sinja , Engedal Nikolai , Cao Shaolong , Handle Florian , Erickson Andrew , Prekovic Stefan , Wetterskog Daniel , Tolonen Teemu , Vuorinen Elisa , Kiviaho Antti , Nätkin Reetta , Häkkinen Tomi , Devlies Wout , Henttinen Sallamari , Kaarijärvi Roosa , Lahnalampi Mari , Kaljunen Heidi , Nowakowska Karolina , Syvälä Heimo , Bläuer Merja , Cremaschi Paolo , Claessens Frank , Visakorpi Tapio , Tammela Teuvo , Murtola Teemu , Granberg Kirsi , Lamb Alastair , Ketola Kirsi , Mills Ian , Attard Gerhardt , Wang Wenyi , Nykter Matti , Urbanucci Alfonso 
Published: April 2021

Abstract
Prostate cancer is heterogeneous and patients would benefit from methods that stratify those who are likely to respond to systemic therapy. We employed single-cell assays for transposase-accessible chromatin (ATAC) and RNA sequencing in models of early treatment response and resistance to enzalutamide. In doing so, we identified pre-existing and treatment-persistent cell subpopulations that possess transcriptional stem-like features and regenerative potential when subjected to treatment. We found distinct chromatin landscapes associated with enzalutamide treatment and resistance that were linked to alternative transcriptional programs. Transcriptional profiles characteristic of persistent stem-like cells were able to stratify the treatment response of patients. Ultimately, we show that defining changes in chromatin and gene expression in single-cell populations from pre-clinical models can reveal hitherto unrecognized molecular predictors of treatment response. This suggests that the high analytical resolution of pre-clinical models enabled by single-cell methods may powerfully inform clinical decision-making.
Up-Regulation of MiR-330-5p is Associated with the Advanced Clinical Stage of Hepatocellular Carcinoma
Authors: Wei Hong-Yu , Huang He-Qing , Chen Gang , Huang Jie-Zhuang , Dang Yi-Wu , Huang Zhi-Guang , Liu Li-Min , hou wei 
Published: April 2021

Abstract
Purpose: A opposite expression levels and roles of miR-330-5p in hepatocellular carcinoma (HCC) have been reported in previous studies, so the clinicopathological implications and the prospective molecular mechanism of miR-330-5p in HCC require elaboration.Materials and methods: To examine the mRNA expression profile of hsa-miR-330-5p in HCC, a in-house RT-qPCR was performed, and the expression data was extracted from sequencing data and gene microarrays from the datasets of The Cancer Genome Atlas (TCGA), Gene Expression Omnibus (GEO), ONCOMINE, ArrayExpress, and Sequence Read Archive (SRA). To have an overview of the clinical value of miR-330-5p, all possible data were integrated to calculate the standard mean difference (SMD) and area under the curve (AUC) from summary receiver operating characteristic curve (sROC). The target genes of miR-330-5p were also predicted and the relative signaling pathways were investigated by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses.Results: According to the integrative analysis, the expression value of miR-330-5p in HCC was higher than in non-tumor tissue (SMD=0.47, 95CI%=0.31-0.63), and high expression of miR-330-5p was related with advanced HCC stage (SMD=0.27, 95CI%=0.08-0.46), poor differentiation (SMD=0.39, 95CI%=0.21-0.58) and poor prognosis (HR=1.91, Log-rank P=0.014). GLUD1, GOT1 and GLS2 were highly likely to be targeted by miR-330-5p, and the progression and prognosis of HCC might be influenced via the miR-330-5p–GLUD1/GOT1/GLS2 axis.Conclusion: The result showed a high-expressed level of miR-330-5p was in the HCC tissue compared with non-tumor tissue, and the overexpression of miR-330-5p indicated poor progression and prognosis in HCC.
Hazardous Base Surges of Taal’s 2020 Eruption
Authors: Lagmay Alfredo Mahar Francisco , Balangue Maria Ines Rosana , Aurelio Mario , Ybanez Audrei Anne , Ybanez Richard , Sulapas Jolly Joyce , Baldago Criselda , Sarmiento Dana Marielle , Cabria Hillel , Rodolfo Raymond , Rafael Dwayne , Trinidad Jose Rainier , Obille Eligio , II Neon Tomas Rossel 
Published: April 2021

Abstract
After 43 years of repose, Taal Volcano erupted on 12 January 2020 forming hazardous base surges. Using field, remote sensing (i.e. UAV and LiDAR), and numerical methods, we gathered primary data to generate well-constrained empirical information on dune bedform characteristics, impact dynamic pressures and velocities of base surges to advance our knowledge on this hazard to understand and evaluate its consequences and risks. The base surges traveled at 50-60 km/hr near the crater and decelerated before making impact on coastal communities with dynamic pressures of at least 1.7-2.1 kPa. The base surges killed more than a thousand livestock in the southeast of Taal Volcano Island, and then traveled another 600 meters offshore. This work is a rare document of a complete, fresh and practically undisturbed base surge deposit, important in the study of dune deposits formed by volcanic, and other processes on Earth and other planets.
Repositioning of a Novel GABA-B Receptor Agonist, AZD3355&nbsp;(Lesogaberan), for the Treatment of Non-alcoholic Steatohepatitis
Authors: Bhattacharya Dipankar , Becker Christine , Readhead Benjamin , Goossens Nicolas , Novik Jacqueline , Fiel Maria Isabel , Cousens Leslie P. , Magnusson Björn , Backmark Anna , Hicks Ryan , Dudley Joel T. , Friedman Scott L. 
Published: April 2021

Abstract
Non-alcoholic steatohepatitis (NASH) is a rising health challenge, with no approved drugs. We used a computational drug repositioning strategy to uncover a novel therapy for NASH, identifying a GABA-B receptor agonist, AZD3355 (lesogaberan) previously evaluated as a therapy for esophageal reflux. AZD3355’s potential efficacy in NASH was tested in human stellate cells, human precision cut liver slices (hPCLS), and in vivo in a well-validated murine model of NASH. In human stellate cells AZD3355 significantly downregulated profibrotic gene and protein expression. Transcriptomic analysis of these responses identified key regulatory nodes impacted by AZD3355, including Myc, as well as MAP and ERK kinases. In PCLS, AZD3355 down-regulated collagen1α1, aSMA and TNF-a mRNA as well as secreted collagen1a1. In vivo, the drug significantly improved histology, profibrogenic gene expression, and tumor development in a robust murine model of NASH, which was comparable to activity of obeticholic acid, an advanced investigational therapy for this disease. These data identify a well-tolerated clinical stage asset as a novel therapy for human NASH through its hepatoprotective, anti-inflammatory and antifibrotic mechanisms of action. The approach validates computational methods to identify novel therapies in NASH in uncovering new pathways of disease development that can be rapidly translated into clinical trials.
Co-Expression Network Revealed the Potential Regulatory Mechanism of Lncrnas in Relapse Hepatocellular Carcinoma.
Authors: Fang Yuan , Yang Yang , Zhang XiaoLi , Li Na , Yuan Bo , Jin Li , Bao Sheng , Li MengGe , Zhao Dan , Li LingRui , Zeng Zhong , Huang HanFei 
Published: April 2021

Abstract
Background: The mechanistic basis for the relapse of hepatocellular carcinoma (HCC) remains poorly understood. Recent research has highlighted the important roles of long non-coding RNAs (lncRNAs) in HCC. However, there are only a few studies on lncRNAs associated with the relapse of HCC.Methods:We analyzed lncRNA and mRNA profiles in the GSE101432 dataset associated with HCC relapse. The differentially expressed lncRNAs and mRNAs were used to construct an lncRNA-mRNA co-expression network. Weighted gene co-expression network analysis followed by Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses were conducted on the database. Furthermore, correlation and survival analyses were performed using The Cancer Genome Atlas database, and the clinical samples were verified by qRT-PCR.Results:In this study, lncRNAs and mRNAs associated with HCC recurrence were identified. Two gene modules were found to be closely linked to HCC relapse. The functional enrichment analysis results of lncRNAs and co-expression mRNAs indicated that they were closely related to the recurrence of HCC. In addition, we verified that the overall survival and recurrence-free survival of these genes in HCC have survival prediction functions. In total, we identified and validated two lncRNAs (LINC00941 and LINC00668) and six mRNAs (LOX, MICB, OTX1, BAIAP2L2, KCTD17, NDUFA4L2) associated with HCC relapse.Conclusion: In summary, we identified the key gene modules and central genes associated with recurrent HCC, and constructed lncRNA-mRNA networks related to this cancer type. These results provide a foundation for future basic research on the mechanism of recurrent liver cancer.
Intraosseous Schwannoma of the Proximal Humarus With Pathologic Fracture
Authors: jiang huajun , Qu Wei , Wu Yuxuan , Yang Jingjing 
Published: April 2021

Abstract
Purpose Intraosseous schwannomas are extremely rare in the humerus, and less than 5 cases have been reported previously in the literature. This is the first report of its origin in the proximal humerus with pathologic fracture. we herein present this case for discussing the reason for its rarity and sharing our experience of management.Case presentation A 55-year-old female patient who presented with pain in the right shoulder, which caused by tripping and falling over a board. Radiographs, computed tomography (CT) and magnetic resonance imaging (MRI) showed considerable tumor in proximal humerus, which connected with a fracture. For this suspected tumor, we performed two operations. Pathological examination demonstrated typical picture of a schwannoma, showing whorls and interlacing fascicles of schwannoma spindle cells. Immunohistochemistry, The tumor cells were diffusely positive for S-100 protein, SOX-10 and CD68, while they were completely negative for desmin, DOG-1, AE1/AE3 and P63. The ki-67 index was about 10%. No mitoses or features of malignancy were identified. Finally, a diagnosis of benign schwannoma with focal of actively proliferated cells was made.Methods The treatment for intraosseous neurilemmomais with pathologic fracture include excisional biopsy, curettage, bone allograft, and fracture fixation. Results The patient recovered well. After the surgery, the patient gradually regained mobility and pain subsided. There was no recurrence after 6 months follow-up by X-ray.Conclusion In our case, the tumor with higher CD68 staining were likely to demonstrate that the tumor volume increase is not only based on cell proliferation, but also intratumoral hemorrhage, vascularization, and inflammation, which may be produce rarefaction of the bone and lead to bone fracture after a trivial trauma.