bluuebunny/crossref_metadata_2025_split huggingface dataset:
Filiform-Fire Needle for Upper Extremity Spastic Paralysis After Stroke: A Randomized Controlled Trial
Authors: Cao Siqiao , Xia Chen , Chen Shenxu , Yu Xiyi , Yuan Ying , Shu Shi , Zhou Qinghui 
Published: May 2021

Abstract
Background: Filiform-fire-needle（FFN）has been used in clinical treatment of upper extremity spastic paralysis after stroke(UESPAS). This study will evaluate the effect of filiform-fire-needle in the treatment of UESPAS under the background of standardized clinical research, and study the relationship between the efficacy and the course of treatment, so as to provide high-quality evidence-based basis for further clinical treatment and future research design.Methods: A prospective randomized controlled study was conducted to evaluate the clinical efficacy of FFN acupuncture therapy for patients with UESPAS. UESPAS patients were recruited in Changhai Hospital, Shanghai, China and they were randomly divided into FFN acupuncture group (F group), filiform needle acupuncture group (A group) or rehabilitation treatment group (R group). During the 20-day treatment, the same acupoints were taken in A group and F group. F group was treated once every 2 days for a total of 10 times, while A group and R group were treated 8 d in a row and then rest for for 2 days for a total of 16 times. In this study, the primary outcome indicators were modified Ashworth scale (MAS), secondary results including Fugl-Meyer assessment of Upper Extremity Motor Function (FMA-UE), National Institutes of Health Stroke Scale (NIHSS), Barthel index (BI). The above scales were evaluated before treatment, 10 days and 20 days after treatment. The safety was evaluated by self-made acupuncture adverse reaction observation table.Results: A significant difference was observed in MAS, FMA-UE, BI, NIHSS in the three groups from pre- to post treatment. From the evaluation of the changes of MAS, the curative effect of F group was better than the other two groups after 10 days of treatment, and F group was better than the R group on the 20th day, but there was no significant difference among the three groups after follow-up. The the difference of FMA-UE score showed F group was higher than the other two groups after 20 days of treatment and follow-up. The change of BI score in group F was higher than that in group R after 10 days of treatment, and the change of BI score in group F was higher than that in the other two groups after 20 days of treatment and follow-up. No significant change in the score of NIHSS was found in three groups.Conclusions: FFN acupuncture therapy has an advantage in relieving upper limb spasm and improving the activities of daily life of stroke patients in the short term, and in the long term, FFN has advantages in improving the motor ability of spastic limbs and improving the ability of daily life of patients.
Influence of rs1292037 Genetic Variant on miR-21 Gene Expression in Patients with type 1 Diabetes Mellitus
Authors: Bayat Reza , Salehi Zivar , Dalili Setila , Bahreini Farbod 
Published: May 2021

Abstract
BackgroundMicroRNAs (miRNAs) are small non-coding RNA molecules that play a pivotal role in the central dogma of molecular biology by regulating gene expression. Alterations in the expression pattern of miRNAs are seen to be linked with several human diseases including autoimmune diseases such as pediatric type 1 diabetes mellitus (T1DM). Single nucleotide polymorphism (SNP) of the miRNAs coding genes can influence pancreatic development and insulin secretion. We contemplated a relation between miR-21 expression level as well as miR-21 rs1292037 SNP and pediatric T1DM.ResultsThe heterozygous T/C genotype was seen to be more common amongst T1DM patients than amongst controls (OR = 2.74 (1.78-4.27), P<0.0001). The C allele was more frequent in patients than in control subjects (OR = 1.36 (1.03-0.8), P = 0.02). miR-21 expression was seen to be upregulated in patients compared to the controls by more than twofold (p<0.0001). In the study population, miR-21 was found to be significantly upregulated when carrying the T/C genotype. Conclusions﻿We report that the miR-21 rs1292037 variant is related to T1DM. Our study also suggests that the miR-21 expression level is upregulated in T1DM patients compared to the control subjects.
Synthesis and Characterization of Some Benzidine-Based Azomethine Derivatives with Molecular Docking Studies and Anticancer Activities
Authors: Erdoğan Musa , yeşildağ ali , Yıldız Barış , Tüzün Burak , Özden Özkan 
Published: May 2021

Abstract
In this paper, a new benzidine-based azomethine derivate 2 with a proposed new mechanism and its two derivatives 4a-b have been designed, synthesized and characterized by 1H, 13C NMR, FT-IR, and HRMS spectroscopic techniques, and their anticancer properties were investigated. The target compounds 2, 4a-b were obtained with excellent yields (91% and above) by condensation of benzidine (1) with three different aldehyde derivatives (formaldehyde, benzaldehyde 3a or p-nitrobenzaldehyde 3b) in refluxing EtOH. Surprisingly, treatment of benzidine (1) with formaldehyde afforded N4,N4,N4',N4'-tetrakis(ethoxymethyl)-[1,1'-biphenyl]-4,4'-diamine (2). The anticancer properties of these benzidine derivatives 2, 4a-b against two cell lines (MDA-MB-231 human breast adenocarcinoma and DLD1 human colorectal adenocarcinoma cell lines) were investigated with a colorimetric assay using the tetrazolium salt WST-8 (2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium, monosodium salts). The obtained results showed that the benzidine-based azomethine derivatives 2, 4a-b had a significant effect against human breast cancer cell line (MDA-MB-231). Then, molecular docking calculations were made to compare the biological activities of benzidine-based azomethine derivatives 2, 4a-b against cancer proteins. ADME/T analysis was performed to examine the drug properties of benzidine-based azomethine derivatives 2, 4a-b. The compounds 2, 4a-b are promising as potential anticancer drug candidates.
The Space-evolution Frame as Alternative to Space-time
Authors: Du Xiaonan 
Published: May 2021

Abstract
As a alternative to Minkowski spacetime frame, this paper propose a four dimensional Euclidean space that combine three spacial dimension with evolution instead of time. It is called space-evolution, in which time is considered as world line length and is absolute.
The space-evolution frame provide a new perspective for understanding of time, space and special relativity. It is
self-consistent
without losing compatibility to special relativity, the Lorentz transform and its predictions could be derived geometrically by simple coordinate rotation.
Relationship Between Tooth Loss and Sarcopenia in Chinese Suburban Community-dwelling Older Adults
Authors: Wang Feng , Wang Jingru , Han Peipei , Liu Yuewen , Ma Weibo , Zhang Hui , Wu Ning , Sang Sijia , Xia Yining , Pan Jiangtao , Liu Yang , Xie Fandi , Niu Shumeng , Hu Hao , Wang Hongbing , Yu Ying , Guo Qi 
Published: May 2021

Abstract
Background: Both sarcopenia and loss of teeth are associated with aging. The purpose of this study was to investigate potential relationships between tooth loss and sarcopenia and components of sarcopenia, including declining muscle mass, muscle strength, and physical performance, in Chinese suburban community-dwelling older adults.Methods: The subjects were 1494 people over 60 years of age (average age: 71.64 ± 5.97 years；men, n=609) from Chongming District of Shanghai and Hangu District of Tianjin. The Asian Working Group for Sarcopenia (AWGS) criteria were used to define sarcopenia. The three basic diagnostic components of sarcopenia (muscle mass, muscle strength, and physical performance) were assessed using a bioelectrical impedance analyzer, a grip strength test, and a four-meter walk test, respectively. The subjects were divided into groups depending on self-reported loss of teeth. Results: After adjusting for confounding variables, we found no correlation between tooth loss and sarcopenia or muscle mass. However, the walking speed of female participants with at least 10 teeth lost was 0.059 m/s slower than that of participants with fewer than 10 teeth lost (P＜0.001), and grip strength was 1.577 kg lower among male participants with at least 10 teeth lost than among males with fewer than 10 teeth lost (P =0.023).Conclusion: Our results suggest that tooth loss is negatively correlated with muscle strength in males and average walking speed in females. These results are consistent with the importance of good oral hygiene in preventing declines of physical performance in older adults.
Discussing the Mechanism of Dahuang Huanglian Xiexin Decoction in the Treatment of Type 2 Diabetes Mellitus via Network Pharmacology and Molecular Docking
Authors: Cen Xi , Wang Yan , Zhang LeiLei , Xue XiaoXiao , Wang Yan , Zhu Xiangdong , Zhang Wei 
Published: May 2021

Abstract
BackgroundType 2 diabetes mellitus (T2DM) is regarded as Pi Dan disease in traditional Chinese medicine (TCM). Dahuang Huanglian Xiexin Decoction (DHXD), a classical TCM formula, has been used for treating Pi Dan disease in clinic, its pharmacological mechanism has not been elucidated. MethodsThis study used network pharmacological analysis and molecular docking approach to explore the mechanism of DHXD on T2DM. Firstly, the compounds in DHXD were obtained from TCMSP and TCMID databases, the potential targets were determined based on TCMSP and UniProt databases. Next, Genecards, Digenet and UniProt databases were used to identify the targets of T2DM. Then, the protein-protein interaction (PPI) network was established with overlapping genes of T2DM and compounds, and the core targets in the network were identified and analyzed. Then, the David database was used for GO and KEGG enrichment analysis. Finally, the target genes were selected and the molecular docking was completed by Autodock software to observe the binding level of active components with target genes.ResultsA total of 397 related components and 128 overlapping genes were identified. After enrichment analysis, it was found that HIF-1, TNF, IL-17 and other signaling pathways, as well as DNA transcription, gene expression, apoptosis and other cellular biological processes had the strongest correlation with the treatment of T2DM by DHXD, and most of them occurred in the extracellular space, plasma membrane and other places, which were related to enzyme binding and protein binding. In addition, 42 core genes of DHXD, such as VEGFA, TP53 and MAPK1, were considered as potential therapeutic targets, indicating the potential mechanism of DHXD on T2DM. Finally, the results of molecular docking showed that HIF-1 pathway had strong correlation with the target genes INSR and GLUT4, quercetin and berberine had the strongest binding power with them respectively.Conclusion﻿This study summarized the main components of DHXD in the treatment of T2DM, identified the core genes and pathways, and systematically analyzed the interaction of related targets, trying to lay the foundation for clarifying the potential mechanism of DHXD on T2DM, so as to carry out further research in the future.
Nanoneedle-forest NiCo2O4@nitrogen-doped Carbon-Mo2C/CC as Double Functional Electrocatalysts for Water Hydrolysis and Zn-air Batteries
Authors: Xu Changhua , Wang Qiguan , Zhao Shaoting , Wang Sumin 
Published: May 2021

Abstract
The construction of efficient and stable non-precious metal electrocatalysts for water hydrolysis and metal-air batteries is a key to tackle the energy shortage and environmental pollution. Herein, a composite consisting of needle-forest nickel cobalt oxide and nitrogen-doped carbon (NDC)-molybdenum carbide loaded on carbon cloth (NiCo2O4@NDC-Mo2C/CC) is synthesized from the hydrothermal method. The introduction of nitrogen-doped carbon and carbon cloth improves the electrical conductivity of the composite, the existence of NDC-Mo2C on CC makes NiCo2O4 produce generous oxygen vacancies. Therefore, NiCo2O4@NDC-Mo2C/CC shows the low overpotentials of HER (120 mV) and OER (220 mV) @10 mA cm-2 and excellent performances for overall water electrolysis in alkaline medium. Also, NiCo2O4@NDC-Mo2C/CC exhibits robust oxygen reduction reaction (ORR) performance and a large specific capacity of 778 mAh g−1 and energy density of 104 mW cm−2 as air cathode in Zn-air batteries. This work enriches knowledge to explore multifunctional electrocatalysts to replace noble metallic catalysts.
How to Promote Learning of Evidence-Based Medicine Among Japanese Medical Students
Authors: Kataoka Yoshihiro , Maeno Takami , Inaba Takashi , Ninn Sayaka , Suzuki Masatsune , Maeno Tetsuhiro 
Published: May 2021

Abstract
Evidence-based medicine (EBM) has become increasingly widespread over the last 30 years. However, the ideal curriculum for undergraduate EBM education has yet to be developed. To establish an EBM curriculum suitable for the educational environment in Japan, we conducted a qualitative study to identify the elements needed to facilitate undergraduate EBM learning among Japanese medical students. Participants were supervising physicians working in teaching hospitals or clinics. Six physicians were interviewed individually from October 2019 to January 2020. The interviewees were asked about their own EBM learning and teaching experiences, what they kept in mind when teaching EBM to medical students and what they felt was needed to improve current undergraduate EBM education. Interview transcripts were analyzed using thematic analysis. Thematic analysis extracted five themes: awareness of foreground questions in clinical practice, motivating learning through observation of role models, awareness of the role of medical students and active learning, understanding patient background as a starting point for practicing EBM, and prioritizing understanding “why” rather than “how” in EBM. Japanese medical students with limited clinical experience may first need to observe their supervisors practice EBM to develop the motivation to learn and grasp the bigger picture of EBM. It is important for medical students to develop an interest in their patients through conversations. Focusing on learning the rationale rather than the skills for practicing EBM may be the key to facilitating initial interest in undergraduate EBM education for subsequent continuous learning.
Identification of Potential Therapeutic Targets For Rheumatoid Arthritis By Bioinformatics
Authors: Du Yi-kuan , Ye Erbai , Xiao Xiaoling , Zhang Senpeng , Ye XinNi , He LuLu , Yang Chun 
Published: May 2021

Abstract
Objective: Based on GEO database, we performed bioinformatics analysis on rheumatoid arthritis (RA)-related gene chips to obtain key genes and signaling pathways of RA, understand the molecular mechanism of RA occurrence and development, and provide candidate targets for the diagnosis and treatment of RA.Methods: The chip GSE77298 related to rheumatoid arthritis in GEO database was retrieved, and the R Programming Language analyzed the differential genes. Subsequently, the differential gene protein-protein interaction (PPI) relationship was constructed. The hub gene was screened, and the DAVID database was used for GO enrichment analysis and KEGG pathway analysis of key differential genes. The miRNAs were then subjected to target gene prediction, and then a miRNA-mRNA visualization network map was constructed using Cytoscape. Finally, transcription factors were predicted by the AnimalTFDB database.Results: ①The chip with the serial number of GSE77298 was retrieved from GEO database, and 1539 differential genes were screened out using the R Programming language analysis, including 1156 up-regulated genes and 383 down-regulated genes. ②By DAVID online functional enrichment analysis of differential genes, it was shown that the signaling pathways were mainly rheumatoid arthritis, Staphylococcus aureus infection, chemokine signaling pathway, viral myocarditis, cytokine-cytokine receptor interaction, etc. ③PPI was constructed through String database. Its hub genes were CXCL12, CD44 and CDH2. The top 10 key differential genes in Degree were CXCL8, PTPRC, MMP9, TLR2, FN1, ITGB2, CXCL1, CCL5, CXCR4 and CXCL10. ④Ten important miRNAs such as hsa-miR-30a-3p, hsa-miR-34a-5p, hsa-miR-30d-3p were predicted. ⑤Transcription factors such as GTF3C2, GLYR1, TRIM24, YY1 were predicted.Conclusion: PI3K-AKT signaling pathway and many other pathways are involved in the occurrence and development of RA, and CXCL8, SOCS3, and TLR2 genes may be the key genes in RA. Ten important miRNAs such as has-miR-340-5p may participate in the pathogenesis of RA. Many transcription factors such as YY1 may be involved in RA's disease process, which will provide directions for further research on RA diagnosis and treatment targets of RA.
Consortium for Blood Group Genes (CBGG): 2008 report
Authors: A. Denomme G. , Westhoff C.M. , Castilho L. , Reid M.E. 
Published: May 2021

Abstract
The Consortium for Blood Group Genes is a worldwide organization whose goal is to have a vehicle to interact, establish guidelines, operate a proficiency program, and provide education for laboratories involved in DNA and RNA testing for the prediction of blood group, platelet, and neutrophil antigens.
Immunohematology

2009;25:75–78.
Lewis blood group system review
Authors: Combs M.R. 
Published: May 2021

Abstract
The expression of Lewis blood group antigens depends on the alleles inherited at two independent loci, FUT2 (Secretor [SE ] gene) and FUT3 (Lewis [LE ] gene). The Se and Le alleles encode separate fucosyltransferases that interact to form Lewis antigens in secretions and fluids. The Lewis antigens on RBCs are not integral to the membrane but are passively adsorbed from the plasma. The antigens are widely distributed in human tissue and fluids and are receptors for some pathogenic bacteria. Lewis antibodies are rarely clinically significant, although there are rare reports of hemolytic transfusion reactions, hemolytic disease of the fetus and newborn, and renal transplant rejection. This review provides a general overview of the Lewis blood group system. An extensive overview by Daniels1 contains additional detailed information on the Lewis blood group system and related antigens.
Historic milestones in the evolution of the crossmatch
Authors: Sandler S.G. , Abedalthagafi M.M. 
Published: May 2021

Abstract
The introduction of the serologic crossmatch—first proposed by Hektoen (1907) and first performed by Ottenberg (1908)—made it possible to transfuse blood without fear of unpredictable and potentially disastrous acute hemolytic reactions, most of which were attributable to direct agglutinating (IgM) anti-A, anti-B, or anti-A,B. Previously transfused or previously pregnant recipients continued to experience sporadic hemolytic transfusion reactions as a result of “incomplete” (IgG) blood group antibodies. Coombs’ introduction of the antiglobulin test (1945) made it possible to detect “incomplete” (IgG) antibodies and to develop laboratory methods to identify and transfuse serologically compatible RBCs. During the past 50 years, the antibody screen has evolved to be more effective than the crossmatch for detecting the presence of potential serologic incompatibility and has, in fact, replaced the crossmatch as the key step in pretransfusion compatibility testing. The antibody screen has become the serologic surrogate for the crossmatch and, currently, the computer crossmatch is becoming the electronic surrogate for the crossmatch. In historical perspective, the past 100 years witnessed the rise and fall of the cross-match. Today, hemolytic transfusion reactions are more likely to be the result of misidentifying the intended transfusion recipient than of failure of routine compatibility testing to detect serologic incompatibility. The introduction of the serologic crossmatch—100 years ago—had a major positive impact on all aspects of clinical medicine. As a result of these advances, we are quickly approaching the limits of improving the safety and efficacy of blood transfusions by conventional serologic methods. Future improvements are more likely to be the result of applications of molecular genotyping—which could not have become available at a more perfect time. When Immunohematology publishes its 50th-anniversary issue, the improvements in transfusion science are more likely to be based on molecular methods than on conventional blood bank serology.
Immunohematology

2009;25:147–151.
Characterization of three novel monoclonal anti-Oka
Authors: Tian M.H. , Halverson G.R. 
Published: May 2021

Abstract
Anti-Oka was first described by Morel and Hamilton in 1979. The Oka antigen has a very high incidence, and only eight probands that are Ok(a–) have been found; all are of Japanese heritage. In this study, we describe the generation and characterization of three novel monoclonal antibodies (Mabs), MIMA-25, MIMA-144, and MIMA-149. The reactivity of these three Mabs was compared with the original human polyclonal anti-Oka. Mice were immunized with transfected HEK cells to induce an immune response, and the spleen B lymphocytes were fused with mouse myeloma X63-Ag8.653 cells to form antibody-secreting hybridomas. The resulting Mabs were tested serologically, by flow cytometry, and by immunoblotting. The specificity of each antibody was determined after excluding specificities to common antigens in the Rh, Kell, Duffy, Kidd, MNS, Lewis, Lutheran, P1, Colton, Diego, Xga, and Dombrock blood group systems. In each case only the Ok(a–) RBC sample was nonreactive. The Mabs and the original human anti-Oka each have a unique pattern of reactivity when tested with enzyme-treated cells; however, none were reactive by immunoblotting. We have generated three novel anti-Oka Mabs: MIMA-144 is an indirectly agglutinating IgG2b antibody, and MIMA-25 and MIMA-149 are directly agglutinating antibodies (IgM and IgA, respectively), underscoring their usefulness as typing reagents for the clinical laboratory.
Immunohematology

2009;25:174–178.
Unexplained agglutination of stored red blood cells in Alsever’s solution caused by the gram-negative bacterium Serratia liquefaciens
Authors: Martincic I. , Mastronardi C. , Chung A. , Ramirez-Arcos S. 
Published: May 2021

Abstract
Alsever’s solution has been used for decades as a preservative solution for storage of RBCs. From October 2005 to January 2006, unexplained hemagglutination of approximately 10 to 20 percent of RBCs stored for several days in a modified version of Alsever’s solution was noticed in quality control testing at the Canadian Blood Services Serology Laboratory. An investigation, including microbial testing,was initiated to determine the cause of the unexplained hemagglutination. The gram-negative bacterium Serratia liquefaciens was isolated from supernatant solutions of agglutinated RBCs. Further characterization of this strain revealed that it has the ability to form biofilms; presents high levels of resistance to chloramphenicol, neomycin, and gentamicin; and causes mannose-sensitive hemagglutination. The source of S. liquefaciens contamination in RBC supernatants was not found. However, this bacterium has not been isolated since January 2006 after enhanced cleaning practices were implemented in the serology laboratory where the RBCs are stored. This biofilm-forming,antibiotic-resistant S.liquefaciens strain could be directly linked to the unexplained hemagglutination observed in stored RBCs.
Immunohematology

2008;24:39–44.