bluuebunny/crossref_metadata_2025_split huggingface dataset:
Autism risk gene POGZ promotes chromatin accessibility and expression of clustered synaptic genes
Authors: Markenscoff-Papadimitriou Eirene , Binyameen Fadya , Whalen Sean , Price James , Lim Kenneth , Catta-Preta Rinaldo , Pai Emily Ling-Lin , Mu Xin , Xu Duan , Pollard Katherine S. , Nord Alex , State Matthew W. , Rubenstein John L. 
Published: April 2021

AbstractDe novomutations inPOGZ, which encodes the chromatin regulatorPogoTransposable Element with ZNF Domain protein, are strongly associated with autism spectrum disorder (ASD). Here we find that in the developing mouse and human brain POGZ binds predominantly euchromatic loci and these are enriched for human neurodevelopmental disorder genes and transposable elements. We profile chromatin accessibility and gene expression inPogz−/−mice and find that POGZ promotes chromatin accessibility of candidate regulatory elements (REs) and the expression of clustered synaptic genes. We further demonstrate that POGZ forms a nuclear complex and co-occupies loci with HP1γ and ADNP, another high-confidence ASD risk gene. InPogz+/−mice,Adnpexpression is reduced. We postulate that reduced POGZ dosage disrupts cortical function through alterations in the POGZ-ADNP balance which modifies neuronal gene expression.
Regulation of Interorganellar Ca2+Transfer and NFAT Activation by the Mitochondrial Ca2+Uniporter
Authors: Yoast Ryan E. , Emrich Scott M. , Zhang Xuexin , Xin Ping , Arige Vikas , Pathak Trayambak , Benson J. Cory , Johnson Martin T. , Lakomski Natalia , Hempel Nadine , Han Jung Min , Dupont Geneviève , Yule David I. , Sneyd James , Trebak Mohamed 
Published: April 2021

AbstractMitochondrial Ca2+uptake is crucial for coupling receptor stimulation to cellular bioenergetics. Further, Ca2+uptake by respiring mitochondria prevents Ca2+-dependent inactivation (CDI) of store-operated Ca2+release-activated Ca2+(CRAC) channels and inhibits Ca2+extrusion to sustain cytosolic Ca2+signaling. However, how Ca2+uptake by the mitochondrial Ca2+uniporter (MCU) shapes receptor-evoked interorganellar Ca2+signaling is unknown. Here, we generated several cell lines with MCU-knockout (MCU-KO) as well as tissue-specific MCU-knockdown mice. We show that mitochondrial depolarization, but not MCU-KO, inhibits store-operated Ca2+entry (SOCE). Paradoxically, despite enhancing Ca2+extrusion and promoting CRAC channel CDI, MCU-KO increased cytosolic Ca2+in response to store depletion. Further, physiological agonist stimulation in MCU-KO cells led to enhanced frequency of cytosolic Ca2+oscillations, endoplasmic reticulum Ca2+refilling, NFAT nuclear translocation and proliferation. However, MCU-KO did not affect inositol-1,4,5-trisphosphate receptor activity. Mathematical modeling supports that MCU-KO enhances cytosolic Ca2+, despite limiting CRAC channel activity.
Safe and effective pool testing for SARS-CoV-2 detection
Authors: Wunsch Marie , Aschemeier Dominik , Heger Eva , Ehrentraut Denise , Krüger Jan , Hufbauer Martin , Syed Adnan S. , Horemheb-Rubio Gibran , Dewald Felix , Fish Irina , Schlotz Maike , Gruell Henning , Augustin Max , Lehmann Clara , Kaiser Rolf , Knops Elena , Silling Steffi , Klein Florian 
Published: April 2021

AbstractBackground / ObjectivesThe global spread of SARS-CoV-2 is a serious public health issue. Large-scale surveillance screenings are crucial but can exceed diagnostic test capacities. We set out to optimize test conditions and implemented high throughput pool testing of respiratory swabs into SARS-CoV-2 diagnostics.Study designIn preparation for pool testing, we determined the optimal pooling strategy and pool size. In addition, we measured the impact of vortexing prior to sample processing, compared pipette- and swab-pooling method as well as the sensitivity of three different PCR assays.ResultsUsing optimized strategies for pooling, we systematically pooled 55,690 samples in a period of 44 weeks resulting in a reduction of 47,369 PCR reactions. In a low prevalence setting, we defined a preferable pool size of ten in a two-stage hierarchical pool testing strategy. Vortexing of the swabs increased cellular yield by a factor of 2.34, and sampling at or shortly after symptom onset was associated with higher viral loads. By comparing different pooling strategies, pipette-pooling was more efficient compared to swab-pooling.ConclusionsFor implementing pooling strategies into high throughput diagnostics, we recommend to apply a pipette-pooling method, using pool sizes of ten samples, performing sensitivity validation of the PCR assays used, and vortexing swabs prior to analyses. Our data shows, that pool testing for SARS-CoV-2 detection is feasible and highly effective in a low prevalence setting.
Heterogenous impairment of α-cell function in type 2 diabetes is linked to cell maturation state
Authors: Dai Xiao-Qing , Camunas-Soler Joan , Briant Linford JB , Santos Theodore dos , Spigelman Aliya F , Walker Emily M. , Arrojo e Drigo Rafael , Bautista Austin , Jones Robert C. , Lyon James , Nie Aifang , Smith Nancy , Fox Jocelyn E Manning , Kim Seung K , Rorsman Patrik , Stein Roland W , Quake Stephen R , MacDonald Patrick E 
Published: April 2021

AbstractIn diabetes, glucagon secretion from pancreatic α-cells is dysregulated. We examined α-cells from human donors and mice using combined electrophysiological, transcriptomic, and computational approaches. Rising glucose suppresses α-cell exocytosis by reducing P/Q-type Ca2+channel activity, and this is disrupted in type 2 diabetes (T2D). Upon high-fat-feeding of mice, α-cells shift towards a ‘β-cell-like’ electrophysiologic profile in concert with an up-regulation of the β-cell Na+channel isoformScn9aand indications of impaired α-cell identity. In human α-cells we identify links between cell membrane properties and cell surface signalling receptors, mitochondrial respiratory complex assembly, and cell maturation. Cell type classification using machine learning of electrophysiology data demonstrates a heterogenous loss of ‘electrophysiologic identity’ in α-cells from donors with T2D. Indeed, a sub-set of α-cells with impaired exocytosis is defined by an enrichment in progenitor markers suggesting important links between α-cell maturation state and dysfunction in T2D.Key findingsα-cell exocytosis is suppressed by glucose-dependent inhibition of P/Q-type Ca2+currentsDysfunction of α-cells in type 2 diabetes is associated with a ‘β-cell-like’ electrophysiologic signaturePatch-seq links maturation state, the mitochondrial respiratory chain, and cell surface receptor expression to α-cell functionα-cell dysfunction occurs preferentially in cells enriched in endocrine lineage markers
Protective host-dependent antagonism amongPseudomonasin theArabidopsisphyllosphere
Authors: Shalev Or , Karasov Talia L. , Lundberg Derek S. , Ashkenazy Haim , Weigel Detlef 
Published: April 2021

AbstractThe plant microbiome is a rich biotic environment, comprising numerous taxa. The community structure of these colonizers is constrained by multiple factors, including host-microbe and microbe-microbe interactions, as well as the interplay between the two. While much can be learned from pairwise relationships between individual hosts and microbes, or individual microbes with themselves, the ensemble of interrelations between the host and microbial consortia may lead to different outcomes that are not easily predicted from the individual interactions. Their study can thus provide new insights into the complex relationship between plants and microbes. Of particular importance is how strain-specific such plant-microbe-microbe interactions are, and how they eventually affect plant health. Here, we test strain-level interactions in the phyllosphere between groups of co-existing commensal and pathogenicPseudomonasamong each other and withA. thaliana, by employing synthetic communities of genome-barcoded isolates. We found that commensalPseudomonasprompted a host response leading to a selective inhibition of a specific pathogenic lineage, resulting in plant protection. The extent of plant protection, however, was dependent on plant genotype, indicating that these effects are host-mediated. There were similar genotype-specific effects on the microbe side, as we could pinpoint an individualPseudomonasisolate as the predominant cause for this differential interaction. Collectively, our work highlights how within-species genetic differences on both the host and microbe side can have profound effects on host-microbe-microbe dynamics. The paradigm that we have established provides a platform for the study of host-dependent microbe-microbe competition and cooperation in theA. thaliana-Pseudomonassystem.
Stakeholders’ views on effective employment support strategies for autistic university students and graduates entering the world of work
Authors: Pesonen Henri , Waltz Mitzi , Fabri Marc , Syurina Elena , Krückels Sarah , Algner Mona , Monthubert Bertrand , Lorenz Timo 
Published: April 2021

Purpose
This paper aims to examine effective support strategies for facilitating the employment of autistic students and graduates by answering the following research question: What constitutes effective employment support for autistic students and graduates?

Design/methodology/approach
Data were collected using the method of empathy-based stories (MEBS) as part of a multinational European project’s Web-based survey. The data consisted of 55 writings about effective strategies and 55 writings about strategies to ]avoid when working with autistic students and graduates. The material was analysed using qualitative inductive content analysis. Narratives were created to illustrate desirable and undesirable environments and processes as they would be experienced by students, supported by original excerpts from the stories.

Findings
The analysis revealed that effective employment support for autistic students and graduates comprised three dimensions of support activity: practices based on the form and environment of support, social interaction support and autism acceptance and awareness. These dimensions were present in both recommended and not recommended support strategy writings.

Originality/value
The results add to the literature on autism and employment with its focus on the novel context of autistic university students and graduates. Effective strategies will be based on person-centred planning, to include not only the individual impact of autism but also individual career goals, workplace characteristics in the chosen field, employer needs and allocation of the right support. There is no one-size-fits-all strategy, but rather an individualized process is needed, focused on the identification of strengths, the adaptation of employment and work processes and improved understanding and acceptance of autism by management, colleagues and administration in the workplace.
An assessment tool for the evaluation of circular economy implementation
Authors: Diéguez-Santana Karel , Rodríguez Rudi Giselle , Acevedo Urquiaga Ana Julia , Muñoz Emanuel , Sablón-Cossio Neyfe 
Published: April 2021

PurposeIn this paper, the authors adopt the theory of the circular economy to study the transitions that take place in three case studies in Mexico and Ecuador. The work is aimed to systematize a circular economy assessment tool that fosters opportunities for improvement in business practices.Design/methodology/approachThe methodology is based on a descriptive quantitative analysis, where a checklist is made with 91 items and nine study variables. This is from the study of the bibliography and business practice. Furthermore, the neural network method is used in a case study to predict the level of circular economy and the importance of each variable according to the sensitivity by the Lek’s profile method.FindingsIt is based on a descriptive quantitative analysis, where a checklist with 91 items and nine study variables is made, defined from a bibliographic study and business practice. Furthermore, the neural network method is used in a case study to predict the level of circular economy and the importance of each variable based on sensitivity.Research limitations/implicationsThe application of the tool requires prior knowledge of the circular economy approach, which is why specialized personnel are needed for its application. This makes research more expensive in time and human resources.Practical implicationsThe practical and methodological contribution of this work lies in the feasibility of the tool that favors the definition of improvement actions for the implementation contribution to the circular economy in business practices.Social implicationsThe social contribution is framed in the gradual transition to circular economy approaches in underdeveloped countries.Originality/valueThe use of the neural network method to predict the level of circular economy in a case study allows making decisions in a predictive way. This encourages the development of the circular economy according to the context needs.
A systematic identification approach for biaxial piezoelectric stage with coupled Duhem-type hysteresis
Authors: Chen Qun , Yang Zong-Xiao , Fu Zhumu 
Published: April 2021

Purpose
The problem of parameter identification for biaxial piezoelectric stages is still a challenging task because of the existing hysteresis, dynamics and cross-axis coupling. This study aims to find an accurate and systematic approach to tackle this problem.

Design/methodology/approach
First, a dual-input and dual-output (DIDO) model with Duhem-type hysteresis is proposed to depict the dynamic behavior of the biaxial piezoelectric stage. Then, a systematic identification approach based on a modified differential evolution (DE) algorithm is proposed to identify the unknown parameters of the Duhem-type DIDO model for a biaxial piezostage. The randomness and parallelism of the modified DE algorithm guarantee its high efficiency.

Findings
The experimental results show that the characteristics of the biaxial piezoelectric stage can be identified with adequate accuracy based on the input–output data, and the peak-valley errors account for 2.8% of the full range in the X direction and 1.5% in the Y direction. The attained results validated the correctness and effectiveness of the presented identification method.

Originality/value
The classical DE algorithm has many adjustment parameters, which increases the inconvenience and difficulty of using in practice. The parameter identification of Duhem-type DIDO piezoelectric model is rarely studied in detail and its successful application based on DE algorithm on a biaxial piezostage is hitherto unexplored. To close this gap, this work proposed a modified DE-based systematic identification approach. It not only can identify this complicated model with more parameters, but also has little tuning parameters and thus is easy to use.
Mobike China: competing through the giant’s ecosystem
Authors: Kittilaksanawong Wiboon , Liu Huijing 
Published: April 2021

Learning outcomes
Students will be able to analyse competitive situations of the focal firm in the platform market, factors that make the focal firm become dominant in the sharing economy through the technology platform and the focal firm’s motives and growth strategies through mergers and acquisitions and overseas expansion, as well as give recommendations on the focal firm’s strategies to move forward to achieve and maintain its competitive position in the platform market.

Case overview/synopsis
On 4th April, 2018, Meituan-Dianping (Meituan), a Chinese group-buying website for consumer products and retail services acquired Mobike, a Chinese dockless bike-sharing platform for US$2.7bn. Mobike had raised several rounds of funding for its large investments and operations in this highly competitive and cash-intensive industry. However, it was still struggling to survive and make a profit in the Chinese and overseas markets. It was believed that the merger between the companies was the only viable alternative. Had Meituan’s Chief Executive Officer made the right decision in acquiring Mobike? After Mobike became an integral part of Meituan, what should be done to turn this technology platform to be profitable in the Chinese and overseas market?

Complexity academic level
The case is intended for senior undergraduate or graduate-level courses in business schools.

Supplementary materials
Teaching Notes are available for educators only.

Subject code
CSS 11: Strategy.
Exploring readiness of learners to transfer training: an interactionist approach
Authors: Ragini  , Ghosh Piyali 
Published: April 2021

PurposePurpose of this study is to investigate the role of learner readiness in enhancing transfer of training by empirically testing a moderated mediation mechanism in which learner readiness influences transfer through motivation to transfer, and this indirect impact is moderated by supervisor support.Design/methodology/approachThe perception of trainees about the constructs considered has been captured through a survey of 250 employees of a unit of a manufacturing organization in India. For hypotheses testing, PROCESS macro developed by Hayes (2013) has been used.FindingsResults have confirmed the significant role played by learner readiness in predicting transfer. This apart, supervisor support has been proved to moderate the indirect impact of learner readiness on transfer.Practical implicationsTrainees need to have pre-requisite knowledge to learn the content of a training programme, which would enable them to grasp such content and transfer the same subsequently to work. It is also essential that trainees are willing to attend any training voluntarily. Specific interventions may be designed for supervisors to bolster their catalytic role in training transfer.Originality/valueAn interactionist approach has been adopted by focussing on learner readiness as a less-studied trainee characteristic and supervisor support as a situational factor of transfer. This is construed as a significant contribution of this study to training literature. The potential overlap between learner readiness and motivation to transfer as trainee characteristics is seen to be neutralized by the presence of supervisor support as a moderator. Findings help in understanding how a trainee’s readiness and motivation, together with supervisor’s positive attitude, can enhance transfer.
The role of human environment in making a workplace congenial: a case of university libraries
Authors: Saleem Qurat Ul Ain , Ameen Kanwal 
Published: April 2021

PurposeOrganizations ought to provide a congenial workplace for employees, particularly females, to get optimum performance. This study aims to investigate the congeniality of libraries’ human environment for female library and information science professionals (LIPs). Regarding the human environment, the manager’s support, relationship with colleagues and informal communication have been studied.Design/methodology/approachFor this quantitative study, the researchers use a survey research method based on a self-constructed questionnaire. The questionnaire consisted of closed-ended questions, which were used to collect the data; however, the open-ended question entitled comments was also added to take the opinion of respondents. The sampling includes the female LIPs working in the university libraries of Punjab, Pakistan.FindingsThe results highlight that female LIPs were satisfied with the human environment at their workplaces. Participants not only appreciated the role and support of their immediate managers in terms of respect, feedback, task allocation and approachability but also recognized the role of colleagues and informal communication (social interaction) in making their workplace congenial and comfortable to work by establishing a positive relationship with supervisors, colleagues or subordinates at the workplace. However, some open-ended comments reveal that females face leg-pulling, favoritism, male colleagues’ indecent behavior and managers’ critical behavior at the workplace.Research limitations/implicationsBecause of the sensitivity of the topic and limitedness of an instrument, the respondents might have been hesitant to give real opinions. This study was limited in terms of participants as data was collected only from one province of Pakistan.Practical implicationsWorkplaces for females in a male-dominated society like Pakistan impede their commitment to giving their best. Despite an increased number of females in the profession, it is surprising that so little empirical research has been conducted on the topic. Therefore, this study is an excellent addition to the dearth of literature on the subject. The findings may help in sensitizing the management and authorities to control the human environment at workplaces as this study will also bring into light the prevailing working environment for females.Originality/valueLibraries as a workplace have been studied internationally (Farler and Broady-Preston, 2012; Ocholla, 2002; Oud, 2008). From the local perspective, the only study concerning the workplace issues of libraries is by Yousafet al.(2013), which was limited to the managerial problems faced by female librarians working at the University of the Punjab. Hence, to the best of the authors’ knowledge, this study will be one of its nature.
Archival Big Data and the Spanish Flu in Copenhagen
Authors: Abildgren Kim 
Published: April 2021

Purpose
The Spanish Flu 1918–1920 saw a high degree of excess mortality among young and healthy adults. The purpose of this paper is a further exploration of the hypothesis that high mortality risk during The Spanish Flu in Copenhagen was associated with early life exposure to The Russian Flu 1889–1892.

Design/methodology/approach
Based on 37,000 individual-level death records in a new unique database from The Copenhagen City Archives combined with approximate cohort-specific population totals interpolated from official censuses of population, the author compiles monthly time series on all-cause mortality rates 1916–1922 in Copenhagen by gender and one-year birth cohorts. The author then analyses birth cohort effects on mortality risk during The Spanish Flu using regression analysis.

Findings
The author finds support for hypotheses relating early life exposure to The Russian Flu to mortality risk during The Spanish Flu. Some indications of possible gender heterogeneity during the first wave of The Spanish Flu – not found in previous studies – should be a topic for future research based on data from other countries.

Originality/value
Due to lack of individual-level death records with exact dates of birth and death, previous studies on The Spanish Flu in Denmark and many other countries have relied on data with lower birth cohort resolutions than the one-year birth cohorts used in this study. The analysis in this paper illustrates how archival Big Data can be used to gain new insights in studies on historical pandemics.