bluuebunny/crossref_metadata_2025_split huggingface dataset:
Short-term transcriptomic response to plasma membrane injury
Authors: Häger Swantje Christin , Dias Catarina , Sønder Stine Lauritzen , Olsen André Vidas , da Piedade Isabelle , Heitmann Anne Sofie Busk , Papaleo Elena , Nylandsted Jesper 
Published: September 2021

AbstractPlasma membrane repair mechanisms are activated within seconds post-injury to promote rapid membrane resealing in eukaryotic cells and prevent cell death. However, less is known about the regeneration phase that follows and how cells respond to injury in the short-term. Here, we provide a genome-wide study into the mRNA expression profile of MCF-7 breast cancer cells exposed to injury by digitonin, a mild non-ionic detergent that permeabilizes the plasma membrane. We focused on the early transcriptional signature and found a time-dependent increase in the number of differentially expressed (> twofold, P < 0.05) genes (34, 114 and 236 genes at 20-, 40- and 60-min post-injury, respectively). Pathway analysis highlighted a robust and gradual three-part transcriptional response: (1) prompt activation of immediate-early response genes, (2) activation of specific MAPK cascades and (3) induction of inflammatory and immune pathways. Therefore, plasma membrane injury triggers a rapid and strong stress and immunogenic response. Our meta-analysis suggests that this is a conserved transcriptome response to plasma membrane injury across different cell and injury types. Taken together, our study shows that injury has profound effects on the transcriptome of wounded cells in the regeneration phase (subsequent to membrane resealing), which is likely to influence cellular status and has been previously overlooked.
Biosynthesis of zinc oxide nanoparticles using Phoenix dactylifera and their effect on biomass and phytochemical compounds in Juniperus procera
Authors: Salih Abdalrhaman M. , Al-Qurainy Fahad , Khan Salim , Tarroum Mohamed , Nadeem Mohammad , Shaikhaldein Hassan O. , Gaafar Abdel-Rhman Zakaria , Alfarraj Norah S. 
Published: September 2021

AbstractBiosynthesized nanoparticles have played vital role recently, as suggested to be alternative to physical and chemical methods. In this study, biosynthesis of zinc oxide nanoparticles (ZnO NPs) were carried out using leaf extracts of Phoenix dactylifera L. and Zinc nitrate. The effect of ZnO nanoparticles on biomass and biochemical parameters was investigated. Biosynthesized ZnO nanostructure was characterized using X-ray diffraction (XRD), transmission electron microscopy (TEM), UV–visible spectrophotometer and Fourier transform infrared spectroscopy (FTIR). Which resulted in spherical shape with size ranging between 16 to 35 nm of Biosynthesized ZnO nanoparticles and UV absorption beak at 370.5 nm with clear peaks of functional groups. The impact of different concentrations (0.0 mg/L, 80 mg/L and 160 mg/L) of biosynthesized ZnO nanoparticles on biomass and bioactive compounds production of Juniperus procera in vitro was investigated. The results showed that, biosynthesized ZnO NPs (80 mg/L and 160 mg/L) concentrations were boosted the growth of J. Procera with significantly compared to non-treated plants in vitro. The highest concentration (160 mg/L) of ZnO NPs was enhanced the growth of plant at beginning period, one month later shoots became yellow and callus turned to be brownish. Moreover, the influence of ZnO NPs on phytochemical compounds in callus of Juniperus procera was examined using GC–MS analysis. The differences among treatments were recoded. Overall, zinc oxide nanoparticles substantially improved the growth of shoots and callus with increasing of biochemical parameters such as chlorophyll a, total phenolic and flavonoids contents, besides the total protein and, SOD, CAT and APX activity. ZnO NPs might be induced some phytochemical compounds as well as inhibit.
Sarcopenia-derived exosomal micro-RNA 16-5p disturbs cardio-repair via a pro-apoptotic mechanism in myocardial infarction in mice
Authors: Hayasaka Taiki , Takehara Naofumi , Aonuma Tatsuya , Kano Kohei , Horiuchi Kiwamu , Nakagawa Naoki , Tanaka Hiroki , Kawabe Jun-ichi , Hasebe Naoyuki 
Published: September 2021

AbstractSarcopenia is a pathophysiological malfunction induced by skeletal muscle atrophy. Several studies reported an association between sarcopenia-induced cardiac cachexia and poor prognosis in heart disease. However, due to lack of an established animal models, the underlying mechanism of disturbed cardiac repair accompanied with sarcopenia remains poorly understood. Here, we developed a novel sarcopenia-induced cardiac repair disturbance mouse model induced by tail suspension (TS) after cardiac ischemia and reperfusion (I/R). Importantly, we identified a specific exosomal-microRNA marker, miR-16-5p, in the circulating exosomes of I/R-TS mice. Of note, sarcopenia after I/R disturbed cardiac repair and raised the level of circulating-exosomal-miR-16-5p secreting from both the atrophic limbs and heart of TS mice. Likewise, miR-16-5p mimic plasmid disturbed cardiac repair in I/R mice directly. Additionally, in neonatal rat ventricular myocytes (NRVMs) cultured in vitro under hypoxic conditions in the presence of a miR-16-5p mimic, we observed increased apoptosis through p53 and Caspase3 upregulation, and also clarified that autophagosomes were decreased in NRVMs via SESN1 transcript interference-mediated mTOR activation. In conclusion, we show the pro-apoptotic effect of sarcopenia-derived miR-16-5p, which may be behind the exacerbation of myocardial infarction. Therefore, miR-16-5p can be a novel therapeutic target in the context of cardiac repair disturbances in sarcopenia–cachexia.
Genetic variants associated with platelet count are predictive of human disease and physiological markers
Authors: Mikaelsdottir Evgenia , Thorleifsson Gudmar , Stefansdottir Lilja , Halldorsson Gisli , Sigurdsson Jon K. , Lund Sigrun H. , Tragante Vinicius , Melsted Pall , Rognvaldsson Solvi , Norland Kristjan , Helgadottir Anna , Magnusson Magnus K. , Ragnarsson Gunnar B. , Kristinsson Sigurdur Y. , Reykdal Sigrun , Vidarsson Brynjar , Gudmundsdottir Ingibjorg J. , Olafsson Isleifur , Onundarson Pall T. , Sigurdardottir Olof , Sigurdsson Emil L. , Grondal Gerdur , Geirsson Arni J. , Geirsson Gudmundur , Gudmundsson Julius , Holm Hilma , Saevarsdottir Saedis , Jonsdottir Ingileif , Thorgeirsson Gudmundur , Gudbjartsson Daniel F. , Thorsteinsdottir Unnur , Rafnar Thorunn , Stefansson Kari 
Published: September 2021

AbstractPlatelets play an important role in hemostasis and other aspects of vascular biology. We conducted a meta-analysis of platelet count GWAS using data on 536,974 Europeans and identified 577 independent associations. To search for mechanisms through which these variants affect platelets, we applied cis-expression quantitative trait locus, DEPICT and IPA analyses and assessed genetic sharing between platelet count and various traits using polygenic risk scoring. We found genetic sharing between platelet count and counts of other blood cells (except red blood cells), in addition to several other quantitative traits, including markers of cardiovascular, liver and kidney functions, height, and weight. Platelet count polygenic risk score was predictive of myeloproliferative neoplasms, rheumatoid arthritis, ankylosing spondylitis, hypertension, and benign prostate hyperplasia. Taken together, these results advance understanding of diverse aspects of platelet biology and how they affect biological processes in health and disease.
Direct tissue-sensing reprograms TLR4+ Tfh-like cells inflammatory profile in the joints of rheumatoid arthritis patients
Authors: Amaral-Silva Daniela , Gonçalves Rute , Torrão Rita C. , Torres Rita , Falcão Sandra , Gonçalves Maria João , Araújo Maria Paula , Martins Maria José , Lopes Carina , Neto Agna , Marona José , Costa Tiago , Castelão Walter , Silva Ana Bento , Silva Inês , Lourenço Maria Helena , Mateus Margarida , Gonçalves Nuno Pina , Manica Santiago , Costa Manuela , Pimentel-Santos Fernando M. , Mourão Ana Filipa , Branco Jaime C. , Soares Helena 
Published: September 2021

AbstractCD4+ T cells mediate rheumatoid arthritis (RA) pathogenesis through both antibody-dependent and independent mechanisms. It remains unclear how synovial microenvironment impinges on CD4+ T cells pathogenic functions. Here, we identified a TLR4+ follicular helper T (Tfh) cell-like population present in the blood and expanded in synovial fluid. TLR4+ T cells possess a two-pronged pathogenic activity whereby direct TLR4+ engagement by endogenous ligands in the arthritic joint reprograms them from an IL-21 response, known to sponsor antibody production towards an IL-17 inflammatory program recognized to fuel tissue damage. Ex vivo, synovial fluid TLR4+ T cells produced IL-17, but not IL-21. Blocking TLR4 signaling with a specific inhibitor impaired IL-17 production in response to synovial fluid recognition. Mechanistically, we unveiled that T-cell HLA-DR regulates their TLR4 expression. TLR4+ T cells appear to uniquely reconcile an ability to promote systemic antibody production with a local synovial driven tissue damage program.
Direct alkylation of N,N-dialkyl benzamides with methyl sulfides under transition metal-free conditions
Authors: Bao Can-Can , Du Hui-Zhen , Luo Yan-Long , Guan Bing-Tao 
Published: September 2021

AbstractAmides are a fundamental and widespread functional group, and are usually considered as poor electrophiles owing to resonance stabilization of the amide bond. Various approaches have been developed to address challenges in amide transformations. Nonetheless, most methods use activated amides, organometallic reagents or transition metal catalysts. Here, we report the direct alkylation of N,N-dialkyl benzamides with methyl sulfides promoted by the readily available base LDA (lithium diisopropylamide). This approach successfully achieves an efficient and selective synthesis of α-sulfenylated ketones without using transition-metal catalysts or organometallic reagents. Preliminary mechanism studies reveal that the deprotonative aroylation of methyl sulfides is promoted by the directed ortho-lithiation of the tertiary benzamide with LDA.
A boron-transfer mechanism mediating the thermally induced revival of frustrated carbene–borane pairs from their shelf-stable adducts
Authors: Hoshimoto Yoichi , Sakuraba Mahiro , Kinoshita Takuya , Ohbo Masaki , Ratanasak Manussada , Hasegawa Jun-ya , Ogoshi Sensuke 
Published: September 2021

AbstractChemists have designed strategies that trigger the conformational isomerization of molecules in response to external stimuli, which can be further applied to regulate the complexation between Lewis acids and bases. We have recently developed a system in which frustrated carbene–borane pairs are revived from shelf-stable but external-stimuli-responsive carbene–borane adducts comprised ofN-phosphine-oxide-substituted imidazolylidenes (PoxIms) and triarylboranes. Herein, we report the detailed mechanism on this revival process. A thermally induced borane-transfer process from the carbene carbon atom to theN-phosphinoyl oxygen atom initiates the transformation of the carbene–borane adduct. Subsequent conformational isomerization via the rotation of theN-phosphinoyl group in PoxIm moieties eventually leads to the revival of frustrated carbene–borane pairs that can cleave H2. We believe that this work illustrates an essential role of dynamic conformational isomerization in the regulation of the reactivity of external-stimuli-responsive Lewis acid-base adducts that contain multifunctional substituents.
Detecting microstructural deviations in individuals with deep diffusion MRI tractometry
Authors: Chamberland Maxime , Genc Sila , Tax Chantal M. W. , Shastin Dmitri , Koller Kristin , Raven Erika P. , Cunningham Adam , Doherty Joanne , van den Bree Marianne B. M. , Parker Greg D. , Hamandi Khalid , Gray William P. , Jones Derek K. 
Published: September 2021

AbstractMost diffusion magnetic resonance imaging studies of disease rely on statistical comparisons between large groups of patients and healthy participants to infer altered tissue states in the brain; however, clinical heterogeneity can greatly challenge their discriminative power. There is currently an unmet need to move away from the current approach of group-wise comparisons to methods with the sensitivity to detect altered tissue states at the individual level. This would ultimately enable the early detection and interpretation of microstructural abnormalities in individual patients, an important step towards personalized medicine in translational imaging. To this end, Detect was developed to advance diffusion magnetic resonance imaging tractometry towards single-patient analysis. By operating on the manifold of white-matter pathways and learning normative microstructural features, our framework captures idiosyncrasies in patterns along white-matter pathways. Our approach paves the way from traditional group-based comparisons to true personalized radiology, taking microstructural imaging from the bench to the bedside.
Charge trapping with α-Fe2O3 nanoparticles accompanied by human hair towards an enriched triboelectric series and a sustainable circular bioeconomy
Authors: Chakraborty Ishita , Lai Sz-Nian , Wu Ming-Chung , Lin Hsun-Yen , Li Chuan , Wu Jyh Ming , Lai Chao-Sung 
Published: September 2021

The PDMS@α-Fe2O3 NP film and human hair-based high performance TENG harvests mechanical energy while expanding waste hair utilization, initiating a rising approach toward the circular bio economy that will play a key role in avoiding climate crisis.
Imatinib protects against human beta-cell death via inhibition of mitochondrial respiration and activation of AMPK
Authors: Elksnis Andris , Schiffer Tomas A , Palm Fredrik , Wang Yun , Cen Jing , Turpaev Kyril , Ngamjariyawat Anongnad , Younis Shady , Huang Suling , Shen Yu , Leng Ying , Bergsten Peter , Karlsborn Tony , Welsh Nils , Wang Xuan 
Published: September 2021

Abstract
The protein tyrosine kinase inhibitor imatinib is used in the treatment of various malignancies but may also promote beneficial effects in the treatment of diabetes. The aim of the present investigation was to characterize the mechanisms by which imatinib protects insulin producing cells. Treatment of non-obese diabetic (NOD) mice with imatinib resulted in increased beta-cell AMP-activated kinase (AMPK) phosphorylation. Imatinib activated AMPK also in vitro, resulting in decreased ribosomal protein S6 phosphorylation and protection against islet amyloid polypeptide (IAPP)-aggregation, thioredoxin interacting protein (TXNIP) up-regulation and beta-cell death. 5-Aminoimidazole-4-carboxamide ribonucleotide (AICAR) mimicked and compound C counteracted the effect of imatinib on beta-cell survival. Imatinib-induced AMPK activation was preceded by reduced glucose/pyruvate-dependent respiration, increased glycolysis rates, and a lowered ATP/AMP ratio. Imatinib augmented the fractional oxidation of fatty acids/malate, possibly via a direct interaction with the beta-oxidation enzyme enoyl coenzyme A hydratase, short chain, 1, mitochondrial (ECHS1). In non-beta cells, imatinib reduced respiratory chain complex I and II-mediated respiration and acyl-CoA carboxylase (ACC) phosphorylation, suggesting that mitochondrial effects of imatinib are not beta-cell specific. In conclusion, tyrosine kinase inhibitors modestly inhibit mitochondrial respiration, leading to AMPK activation and TXNIP down-regulation, which in turn protects against beta-cell death.
Photometric survey of 55 near-earth asteroids
Authors: Hromakina T. , Birlan M. , Barucci M. A. , Fulchignoni M. , Colas F. , Fornasier S. , Merlin F. , Sonka A. , Petrescu E. , Perna D. , Dotto E. ,   the NEOROCKS Team
Published: September 2021

Context. Near-earth objects (NEOs), thanks to their proximity, provide a unique opportunity to investigate asteroids with diameters down to dozens of meters. The study of NEOs is also important because of their potential hazard to the Earth. The investigation of small NEOs is challenging from Earth as they are observable only for a short time following their discovery and can sometimes only be reached again years or decades later.
Aims. We aim to derive the visible colors of NEOs and perform an initial taxonomic classification with a main focus on smaller objects and recent discoveries.
Methods. Photometric observations were performed using the 1.2 m telescope at the Haute-Provence observatory and the 1.0 m telescope at the Pic du Midi observatory in broadband Johnson-Cousins and Sloan photometric systems.
Results. We present new photometric observations for 55 NEOs. Our taxonomic classification shows that almost half (43%) of the objects in our sample are classified as S+Q-complex members, 19% as X-complex, 16% as C-complex, 12% as D-types, and finally 6% and 4% as A- and V-types, respectively. The distribution of the observed objects with H > 19 and H ≤ 19 remains almost the same. However, the majority of the objects in our dataset with D < 500 m belong to the “silicate” group, which is probably a result of an observational bias towards brighter and more accessible objects. “Carbonaceous” objects are predominant among those with a Jovian Tisserand parameter of Tj < 3. These bodies could be dormant or extinct comets. The median values of the absolute magnitude for “carbonaceous” and “silicate” groups are H = 18.10 ± 0.95 and H = 19.50 ± 1.20, whereas the estimated median diameters are D = 1219 ± 729 m and D = 344 ± 226 m, respectively. “Silicate” objects have a much lower median Earth’s minimum orbit intersection distance (MOID) and a somewhat lower orbital inclination in comparison to “carbonaceous” objects. About half of the observed objects are potentially hazardous asteroids and are mostly (almost 65%) represented by “silicate” objects.
Exploring deep and hot adiabats as a potential solution to the radius inflation problem in brown dwarfs
Authors: Sainsbury-Martinez F. , Casewell S. L. , Lothringer J. D. , Phillips M. W. , Tremblin P. 
Published: September 2021

Aims. The anomalously large radii of highly irradiated gaseous exoplanets has remained a mystery for some time. One mechanism that has been suggested as a solution for hot Jupiters is the heating of the deep atmosphere via the vertical advection of potential temperature, resulting in increased internal entropy. In this work, we intend to explore whether this mechanism can also explain the observed brown dwarf radii trend: a general increase in the observed radius with irradiation, with an exception, however, for highly irradiated brown dwarfs orbiting white dwarfs.
Methods. We used a 3D global circulation model (GCM) known as DYNAMICO to run a series of long-timescale models of the deep atmospheres of Kepler-13Ab, KELT-1b, and SDSS1411B. These models allowed us to explore not only whether a stable advective adiabat can develop in this context, but also to consider the associated dynamics.
Results. We find that our brown dwarf models fall into two distinct regimes. First, Kepler-13Ab and KELT-1b both show signs of significant deep heating and, hence, are able to maintain adiabats that are hotter than 1D models predict. On the other hand, SDSS1411B exhibits a much weaker downward heating profile that not only struggles to heat the interior under ideal conditions, but is highly sensitive to the presence of deep radiative dynamics.
Conclusions. We conclude that the vertical advection of potential temperature by large-scale atmospheric circulations constitutes a robust mechanism to explain the trend of increasing inflation with irradiation. This includes an exception for highly irradiated brown dwarfs orbiting white dwarfs, which can be understood as occurring due to the role that increasing rotational influence plays in the context of mid-to-high latitude advective dynamics. Furthermore, when paired with a suitable parametrisation of the outer atmosphere irradiation profile, this mechanism alone could potentially provide a complete explanation for the observed levels of radius inflation in our brown dwarf sample. Finally, in order to confirm the validity of this explanation, we suggest that this work should be followed by future studies of brown dwarfs atmospheres using next-generation, fully radiative GCMs.
Wurzelresorptionen permanenter Zähne im Kindes- und Jugendalter
Authors: Hirsch Christian 
Published: September 2021

ZusammenfassungIm Unterschied zur physiologischen Resorption der Milchzähne stellt die Resorption permanenter Zähne immer einen pathologischen Prozess dar, der oft zum Verlust der betroffenen Zähne führt. Das Ausmaß des Phänomens ist nicht genau bekannt, da es sich bei Berichten in der Literatur meist um einzelne Kasuistiken handelt und systematische radiologische Studien nicht existieren. Für den Praktiker ist die wesentliche Frage die Abschätzung der Prognose für die betroffenen Zähne.
Gewinnung von Stammzellen aus Nabelschnurblut: Relevanz peripartaler
Faktoren für die Qualität von Transplantaten
Authors: Farina-Eckhardt Patricia , Granado Cristina , Mueller-Borer Doris , Schötzau Andreas , Tsakiris Dimitrios A. , Hösli Irene , Manegold-Brauer Gwendolin 
Published: September 2021

Zusammenfassung
Einleitung Nabelschnurblut (NSB) enthält hämatopoetische
Stammzellen mit therapeutischem Potenzial und einzigartigen zellulären
Eigenschaften. Aufgrund der begrenzten Anzahl an Stammzellen im NSB
(Surrogatmarker total nucleated cells, TNC) eignet sich nur jede fünfte
Spende für eine Transplantation. Ziel dieser Studie war es, zu
untersuchen, ob prädiktive Faktoren für eine TNC-Zahl
über der 99. Perzentile existieren.
Material und Methodik Retrospektive Datenanalyse der 100
größten NSB-Spenden (Top100-Kohorte) aus 2299 registrierten
Einheiten. Unterschiede zwischen maternalen, fetalen und geburtshilflichen
Faktoren wurden analysiert und mit einer standardisierten Kohorte von 731
NSB-Spenden verglichen.
Ergebnisse Das mütterliche Alter und der BMI in der Top100-Kohorte
waren höher als in der Vergleichskohorte (32 vs. 31 Jahre,
p=0,007; 30 kg/m2 vs. 29 kg/m2,
p=0,024). Es gab mehr Erstgebärende (76,0 vs. 62,8%,
p=0,013) und Gestationsdiabetikerinnen (5,00 vs. 1,65%,
p=0,044). Die Schwangerschaftswoche, das Geburtsgewicht, der Anteil
vaginal-operativer Geburten und sekundärer Sectiones war in der
Top100-Kohorte höher (40+4 vs. 40+1 SSW,
p=0,002), (3700 vs. 3450 g, p<0,001), (53,0 vs.
22,7%, p<0,001) (10 vs. 6,2%, p=0,014).
Fazit Für eine erfolgreiche Transplantation ist die Höhe
der TNC-Zahl entscheidend. Vaginal-operative Entbindungen, sekundäre
Sectiones und ein Geburtsgewicht über 3700 g sind
günstige Faktoren. Gerade bei Geburten mit einem pathologischen Verlauf
sollte nach sicherer Versorgung von Mutter und Kind nicht auf eine Entnahme
verzichtet werden.
Synthesis of a New Phorbazole and Its Derivatives
Authors: Muderawan I Wayan , Young David J. , Loughlin Wendy A. 
Published: September 2021

AbstractPhorbazoles are chlorinated marine alkaloids containing pyrrole, oxazole and phenol ring units, and differ in the number and positions of chlorine atoms. They are isolated from sea sponges and nudibranchs. In this work, a convenient synthetic method leading to a new phorbazole and its derivatives is developed. This synthesis of synthetic phorbazole G and its derivatives is achieved in seven steps in good overall yields of 26–52%. It involves formation of the pyrrole-oxazole skeleton followed by chlorination. The pyrrole-oxazole skeleton is synthesized from pyrrole and substituted acetophenones, and the key step involves cyclodehydration of amide intermediates to give protected oxazoles, followed by hydrolysis.