bluuebunny/crossref_metadata_2025_split huggingface dataset:
Empowering nurses in angola: the transformative outcome of training pioneer heroes / Empoderando enfermeiros em angola: as transformações resultantes da educação de heróis pioneiros
Authors: Mendes Isabel Amelia Costa , Ventura Carla Aparecida Arena , Marchi-Alves Leila Maria , Silva Ítalo Rodolfo , Godoy Simone , Almeida Emerson Willian Santos 
Published: June 2021

Objetivo: Revisitar a história de cooperação de dois países de língua portuguesa, que resultou na formação dos primeiros enfermeiros de Angola ; Caracterizar os alunos angolanos que participaram do acordo de cooperação ; Identificar suas perspectivas e sentimentos ao final do programa, as influências da educação recebida, motivações para continuarem estudando e satisfação no trabalho. Método: estudo exploratório conduzido com alunos angolanos que graduados em Escola de Enfermagem pública do Brasil. Os dados foram coletados por meio de questionário desenvolvido pelos pesquisadores, culturalmente validado com dois especialistas enfermeiros de Angola. Resultados: os esforços, desafios e realizações de heróis de ambos os lados do Atlântico são reconhecidos e se refletem na realidade social do país africano. Conclusão: Acordos de cooperação são recomendados como um caminho para a sustentabilidade das políticas de saúde a partir de uma perspectiva global.
Ribosome‐associated quality control mediates degradation of the premature translation termination product Orf1p of ODC antizyme mRNA
Authors: Pradhan Ashis Kumar , Kandasamy Ganapathi , Chatterjee Upasana , Bharadwaj Anushree , Mathew Sam J. , Dohmen R. Jürgen , Palanimurugan R. 
Published: June 2021

Decoding ofOAZ1(Ornithine decarboxylaseAntiZyme 1) mRNA, which harbours two open reading frames (ORF1 and ORF2) interrupted by a naturally occurring Premature Termination Codon (PTC), produces an 8 kDa truncated polypeptide termed Orf1p, unless the PTC is bypassed by +1 ribosomal frameshifting. In this study, we identified Orf1p as an endogenous ubiquitin‐dependent substrate of the 26S proteasome both in yeast and mammalian cells. Surprisingly, we found that the ribosome‐associated quality control factor Rqc1 and the ubiquitin ligase Ltn1 are critical for Orf1p degradation. In addition, the cytosolic protein quality control chaperone system Hsp70/Hsp90 and their corresponding co‐chaperones Sse1, Fes1, Sti1 and Cpr7 are also required for Orf1p proteolysis. Our study finds that Orf1p, which is naturally synthesized as a result of a premature translation termination event, requires the coordinated role of both ribosome‐associated and cytosolic protein quality control factors for its degradation.
Confidence Intervals for Two Independent Binomial Proportions
Authors: Fagerland Morten W. , Lydersen Stian , Laake Petter 
Published: June 2021

AbstractNumerous confidence intervals for two independent binomial proportions exist. This article presents several intervals – both well‐known ones and less commonly used ones – for three effect measures: difference between proportions, ratio of proportions, and odds ratio. Many of the commonly used intervals perform poorly, especially in small samples, but with just a small amount of additional effort, more precise and reliable intervals can be obtained. This article provides recommendations and illustrates the methods on data from a clinical trial.
Microfluidic Printing of Tunable Hollow Microfibers for Vascular Tissue Engineering
Authors: Wu Zhuhao , Cai Hongwei , Ao Zheng , Xu Junhua , Heaps Samuel , Guo Feng 
Published: June 2021

AbstractBioprinting of vascular tissues holds great potential in tissue engineering and regenerative medicine. However, challenges remain in fabricating biocompatible and versatile scaffolds for the rapid engineering of vascular tissues and vascularized organs. Here, novel bioink‐enabled microfluidic printing of tunable hollow microfibers is reported for the rapid formation of blood vessels. By compositing biomaterials including sodium alginate, gelatin methacrylate, and glycidyl‐methacrylate silk fibroin, a novel composite bioink with excellent printability and biocompatibility is prepared. This composite bioink can be printed into hollow microfibers with tunable dimensions using a microfluidic co‐axial printing device. After seeding human umbilical vein endothelial cells into the hollow chambers via a microfluidic perfusion device, these cells can adhere to, grow, proliferate, and then cover the internal surface of the printed hollow scaffolds to form vessel‐like tissue structures within 3 days. By combining the unique composite bioink, microfluidic printing of vascular scaffolds, and microfluidic cell seeding and culturing, the strategy can rapidly fabricate vascular‐like tissue structures with high viability and tunable dimensions. The presented method may engineer in vitro vasculatures for the broad applications in basic research and translational medicine including in vitro disease models, tissue microcirculation, and tissue transplantation.
UV‐Shielding and Clusteroluminogenic Cellulose‐Based Films with Tuneable Wettability and Permeability for Dually Self‐Indicating Food Packaging
Authors: Lai Wing‐Fu , Yip Wan , Wong Wing‐Tak 
Published: June 2021

AbstractFood packaging plays a vital role not only in extending the shelf‐life and ensuring the safety and quality of food, but also in reducing the occurrence of food loss and waste. This study reports the generation of biocompatible and tuneable cellulose‐based films, which show strong UV absorbing capacity in both UVA and UVB regions to protect the packaged food from UV degradation. By taking advantage of the process of clusterization‐triggered emission, the films can indicate, by displaying changes in the intensity of luminescence, not only their properties but also the status of the packaged food. The protective and dually self‐indicating capacity of the films in food packaging is demonstrated by using frozen chicken breast as a food model. Together with their negligible toxicity, good barrier properties, and high tunability, the films warrant further development as dually self‐indicating materials for food packaging applications.
tert‐Butyl Hydroperoxide‐Initiated Radical Cyclization of 1‐(Allyloxy)‐2‐(1‐Arylvinyl)Benzenes with Sulfinic Acids to Access Sulfonated Benzoxepines
Authors: Zhou Nengneng , Kuang Kaimo , Wu Meixia , Wu Sixin , Xu Qiankun , Xia Ziqin , Zhang Man 
Published: June 2021

AbstractA tert‐butyl hydroperoxide‐initiated radical cyclization of 1‐(allyloxy)‐2‐(1‐arylvinyl)benzenes with sulfinic acids for the construction of sulfonated benzoxepines is developed. This reaction involves a radical pathway and offers a straightforward route to the formation of seven‐membered ring via sulfonylation/cyclization process. This methodology features mild reaction conditions, a broad substrate scope and good functional group tolerance.magnified image
LET’s CONNECT Community Mentorship Program for Adolescents with Peer Social Problems: A Randomized Intervention Trial
Authors: King Cheryl A. , Gipson Polly Y. , Arango Alejandra , Lernihan Deanna , Clark Michael , Ewell Foster Cynthia , Caldwell Cleopatra , Ghaziuddin Neera , Stone Deborah 
Published: June 2021

AbstractThis study examined the effectiveness of LET’s CONNECT (LC), a community mentorship program based on the positive youth development model. Participants were 218 youth (66.5% girls), ages 12 to 15, who reported peer victimization, bullying perpetration, and/or low social connectedness. These youth were randomized to LC or the control group (community resource information). The LC program linked youth to community mentors who connected with youth and facilitated their involvement in social growth activities across a 16‐month period. Outcomes were assessed at 6 and 16 months with self‐report measures of social and community connectedness, thwarted belongingness, depression, self‐esteem, and suicidal ideation and behavior. In intent‐to‐treat analyses, LC was associated with modest positive effects for social connectedness, self‐esteem, and depression. It had no effects on suicidal ideation or behavior. Results suggest that LC has the potential to positively impact the developmental trajectories of youth dealing with the interpersonal challenges of victimization, bullying perpetration, or low social connectedness. LC implementation challenges and directions for further research are also discussed.
Organocatalytic Cascade Synthesis of Peroxy‐Substituted Cyclic Carbonates from CO2‐Sourced α‐Alkylidene Cyclic Carbonates and Hydroperoxides
Authors: Zhou Hui , Zhang Yi‐Feng , Chen Wei , Zhang Wen‐Zhen , Lu Xiao‐Bing 
Published: June 2021

AbstractThe first cascade reaction between CO2‐sourced α‐alkylidene cyclic carbonates and hydroperoxides is reported. This reaction proceeds smoothly under mild reaction conditions using 1,5,7‐triazabicyclo[4.4.0]dec‐5‐ene as an effective commercially available organocatalyst, thus providing a sustainable methodology for the formation of a wide variety of peroxy‐substituted cyclic carbonates in moderate to high yields. Control experiments, in situ FTIR measurements, and density functional theory calculations reveal that the catalytic reaction is a three‐step process, including intermolecular transesterification, peroxyhemiacetalization, and intramolecular transesterification, with the second step being rate determining.
Chiral Induction in Buckminsterfullerene Using a Metal–Organic Framework
Authors: Lo Shao‐Wei , Kitao Takashi , Nada Yusuke , Murata Kei , Ishii Kazuyuki , Uemura Takashi 
Published: June 2021

AbstractChiral induction is an emerging topic of interest in various areas of chemistry because of its relationship to the elusive mechanism of spontaneous symmetry breaking in nature. Buckminsterfullerene (C60) with the shape of a highly symmetric truncated icosahedron has rarely been referred for chiral induction due to the difficult symmetry breaking. In this work, we demonstrate that a chiral metal–organic framework (MOF) can provide a key field for chiral induction. C60 could be incorporated into the chiral nanochannels of the MOF using an in situ self‐assembly strategy. The circular dichroism spectra of the resulting nanocomposites showed an intense chiral signal in the absorption region of C60. Experimental and theoretical studies showed that this unprecedented chiral induction of C60 was attributed to hybridization of the molecular orbitals through a close association with the pore surface of the MOF. Our method can endow highly symmetric achiral compounds with chirality, paving the new way toward fabrication of novel chiral nanomaterials.
Highly Selective CO2 Conversion to Methanol in a Bifunctional Zeolite Catalytic Membrane Reactor
Authors: Yue Wenzhe , Li Yanhong , Wei Wan , Jiang Jianwen , Caro Jürgen , Huang Aisheng 
Published: June 2021

AbstractThe hydrogenation of sequestrated CO2 to methanol can reduce CO2 emission and establish a sustainable carbon circuit. However, the transformation of CO2 into methanol is challenging because of the thermodynamic equilibrium limitation and the deactivation of catalysts by water. In the present work, different reactor types have been evaluated for CO2 catalytic hydrogenation to methanol. Best results have been obtained in a bifunctional catalytic membrane reactor (CMR) based on a zeolite LTA membrane and a catalytic Cu‐ZnO‐Al2O3‐ZrO2 layer on top. Due to the in situ and rapid removal of the produced water from the catalytic layer through the hydrophilic zeolite LTA membrane, it is effective to break the thermodynamic equilibrium limitation, thus significantly increasing the CO2 conversion (36.1 %) and methanol selectivity (100 %). Further, the catalyst deactivation by the produced water can be effectively inhibited, thus maintaining a high long‐term activity of the CMR.
Deformylierung von 5‐Formylcytidin in unterschiedlichen Zelltypen
Authors: Korytiaková Eva , Kamińska Ewelina , Müller Markus , Carell Thomas 
Published: June 2021

AbstractDie epigenetische Programmierung von Zellen erfordert die Methylierung von Desoxycytidinen (dC) zu 5‐Methyl‐dC (mdC) mit anschließender Oxidation zu 5‐Hydroxymethyl‐dC (hmdC), 5‐Formyl‐dC (fdC) und 5‐Carboxy‐dC (cadC). Die anschließende Transformation von fdC und cadC zurück zu dC etabliert einen chemischen intragenetischen Regelkreis. Einer der diskutierten Wege enthält die Tdg‐unabhängige Deformylierung von fdC direkt zu dC. Hier berichten wir über die Synthese einer fluorierten fdC‐Fütterungssonde (F‐fdC), um die direkte Deformylierung zum F‐dC zu untersuchen. Die Synthese wurde entlang eines neuartigen Weges durchgeführt, der jegliches F‐dC als Zwischenprodukt umgeht, um Kontaminationsstörungen zu vermeiden. Die Zufuhr von F‐fdC und die Beobachtung der F‐dC‐Bildung in vivo gaben Einblicke in den Tdg‐unabhängigen Umwandlungsprozess. Wir liefern Daten, die die Deformylierung auch in somatischen Zelltypen belegen, nicht nur in Stammzellen. Wir untersuchten zudem die aktive Demethylierung in einem nicht‐teilenden Neurogenin‐induzierbaren System von iPS‐Zellen, die sich zu bipolaren Neuronen differenzieren.
Deformylation of 5‐Formylcytidine in Different Cell Types
Authors: Korytiaková Eva , Kamińska Ewelina , Müller Markus , Carell Thomas 
Published: June 2021

AbstractEpigenetic programming of cells requires methylation of deoxycytidines (dC) to 5‐methyl‐dC (mdC) followed by oxidation to 5‐hydroxymethyl‐dC (hmdC), 5‐formyl‐dC (fdC), and 5‐carboxy‐dC (cadC). Subsequent transformation of fdC and cadC back to dC by various pathways establishes a chemical intra‐genetic control circle. One of the discussed pathways involves the Tdg‐independent deformylation of fdC directly to dC. Here we report the synthesis of a fluorinated fdC feeding probe (F‐fdC) to study direct deformylation to F‐dC. The synthesis was performed along a novel pathway that circumvents any F‐dC as a reaction intermediate to avoid contamination interference. Feeding of F‐fdC and observation of F‐dC formation in vivo allowed us to gain insights into the Tdg‐independent removal process. While deformylation was shown to occur in stem cells, we here provide data that prove deformylation also in different somatic cell types. We also investigated active demethylation in a non‐dividing neurogenin‐inducible system of iPS cells that differentiate into bipolar neurons.
Fabrication of fibrous poly (ɛ‐caprolactone) nano‐fibers containing cerium doped‐bioglasses nanoparticles encapsulated collagen
Authors: Sahrapeyma Hamed , Asefnejad Azadeh , Azami Mahmoud , Sadroddiny Esmaeil 
Published: June 2021

AbstractNovel nanosized designed ceramic powders, cerium (Ce) doped bioglass (BG) with various doped Ce content, were synthesized by sol–gel method in order to be employed in the development of PCL fibrous scaffold for bone tissue engineering applications. Characterization techniques such as X‐ray diffraction analysis, transmission electron microscopy, Fourier transform infrared spectroscopy, and energy‐dispersive X‐ray spectroscopy were employed to evaluate the developed Ce doped BG powders. The results confirmed successful doping of Ce inside BG structure. 0, 1, 3, and 10 wt% Ce doped 58S BG were successfully encapsulated in the collagen microspheres by water‐in‐oil emulsion method and the average particle size and hydrodynamic diameter of microspheres were determined using scanning electron microscopy and dynamic light scattering analysis, respectively. Next, 0, 1, 3, and 10 wt% Ce doped 58S BG encapsulated collagen microspheres were loaded inside the Poly(ɛ‐caprolactone) fibrous scaffold and their in vitro bioactivity and biocompatibility properties were evaluated. The results of soaking samples in the simulated body fluid showed that all Ce doped 58S BG encapsulated collagen microspheres loaded PCL fibrous scaffold have acceptable bioactivity and apatite formation ability over time. The biocompatibility evaluation of developed scaffolds showed high viability and proliferation of MG63 cells cultured on the surface of 3% Ce doped 58S BG encapsulated collagen microsphere loaded in the PCL fibrous scaffold and its high potential ability for bone tissue engineering applications. These results potentially open new aspects for scaffolds aimed at the regeneration of bone defects.
A rapid RP‐HPLC stability‐indicating method development and validation of moxifloxacin hydrochloride–related substances in finished dosage forms
Authors: Vankalapati Krishna Rao , Algete Pallavi , Boodida Sathyanarayana 
Published: June 2021

AbstractA reversed‐phase high‐performance liquid chromatography (RP‐HPLC) method was developed and validated for the identification and quantification of moxifloxacin hydrochloride–related substances in finished dosage forms. Chromatographic separation was achieved on an Agilent C18 column (150 × 4.6 mm, 5 μm) with the mobile phase (0.01 M potassium dihydrogen orthophosphate as buffer and methanol in the ratio of 70:30) eluted in isocratic mode. The HPLC flow rate was 1.0 mL min−1, and peaks were monitored at 230 nm using a photodiode array (PDA) detector. The column temperature was kept constant at 30°C, and the injection volume was 10 μL. The run time of the method was 16 min. The method was validated as per the International Conference on Harmonization (ICH) guidelines. Linearity was recorded at various concentrations ranging from 0.25 to 1.5 μg mL−1 for all the moxifloxacin impurities. Linearity, regression value, recovery, %relative standard deviation (RSD) of method precision values were found within the acceptance limits. The method for related substances (RS) in moxifloxacin was found to be specific, linear, accurate, precise, rugged, and robust. The validated method was suitable for the quantification of the RSs in moxifloxacin drug products. The method was applied in quality control lab for the analysis of moxifloxacin impurities in stability analysis.
Development and validation of a stability‐indicating UPLC method for the determination of olmesartan medoxomil, amlodipine and hydrochlorothiazide degradation impurities in their triple‐combination dosage form using factorial design of experiments
Authors: Marisetti Vishnu Murthy , Katakam Prakash , Siddhani Vijaya Krishna , Katari Naresh Kumar 
Published: June 2021

AbstractThe current work describes the development and validation of a stability‐indicating UPLC method for the determination of olmesaratan medoxomil (OLM), amlodipine besylate (AMB), hydrochlorothiazide (HCT) and their degradation products in the triple‐combination tablet dosage form. The separation was achieved using a Zorbax Eclipse plus C8 RRHD (100 mm × 3.0 mm), 1.8 μm column with gradient elution of mobile phase A containing 0.02 m of sodium phosphate buffer (pH 3.35) and mobile phase B as acetonitrile and water (90:10, v/v). The detector signal was monitored at UV 250 nm. Analytical performance of the optimized UPLC method was validated as per International Conference on Harmonization guidelines. The linearity ranges for OLM, AMB and HCT were 0.59–240, 0.30–60 and 0.37–150 μg/ml, respectively, with correlation coefficients >0.999. The dosage form was subjected to forced‐degradation conditions of neutral, acidic and alkaline hydrolysis, oxidation and thermal and photodegradation. The method was proved to be stability indicating by demonstrating the specificity of the drugs from degradation products. The robustness of the method was evaluated through a two‐level, three‐factorial design with a multivariate approach. Statistical data analysis with best model fit P‐value < 0.05 from an ANOVA test indicated that the influence of individual factors is relatively higher than the interaction effects. The method is useful for the analysis of drug products.
Cognitive and behavioral associated changes in manifest Huntington disease: A retrospective cross‐sectional study
Authors: Migliore Simone , D’Aurizio Giulia , Maffi Sabrina , Ceccarelli Consuelo , Ristori Giovanni , Romano Silvia , Castaldo Anna , Mariotti Caterina , Curcio Giuseppe , Squitieri Ferdinando 
Published: June 2021

AbstractIntroductionBehavioral and cognitive changes can be observed across all Huntington disease (HD) stages. Our multicenter and retrospective study investigated the association between cognitive and behavioral scale scores in manifest HD, at three different yearly timepoints.MethodsWe analyzed cognitive and behavioral domains by the Unified Huntington's Disease Rating Scale (UHDRS) and by the Problem Behaviors Assessment Short Form (PBA‐s), at three different yearly times of life (t0 or baseline, t1 after one year, t2 after two years), in 97 patients with manifest HD (mean age 48.62 ± 13.1), from three ENROLL‐HD Centers. In order to test the disease progression, we also examined patients’ motor and functional changes by the UHDRS, overtime.ResultsThe severity of apathy and of perseveration/obsession was associated with the severity of the cognitive decline (p < .0001), regardless of the yearly timepoint. The score of irritability significantly and positively correlated with perseveration errors in the verbal fluency test at t0 (r = .34; p = .001), while the psychosis significantly and negatively correlated with the information processing speed at t0 (r = −.21; p = .038) and significantly and positively correlated with perseveration errors in the verbal fluency test at t1 (r = .35; p < .0001).The disease progression was confirmed by the significant worsening of the UHDRS‐Total Motor Score (TMS) and of the UHDRS‐Total Functional Capacity (TFC) scale score after two‐year follow‐up (p < .0001).ConclusionAlthough the progression of abnormal behavioral manifestations cannot be predicted in HD, the severity of apathy and perseveration/obsessions are significantly associated with the severity of the cognitive function impairment, thus contributing, together, to the disease development and to patients’ loss of independence, in addition to the neurological manifestations. This cognitive‐behavior pattern determines a common underlying deficit depending on a dysexecutive syndrome.