bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comparative Proteomics of Schistosoma Japonicum Developed From Different Oncomelania Snails Permissive Areas
Authors: Miao Feng , Wang Yongbin , Yang Kun , Li Wei , Xiong Chunrong , Zhang Jianfeng , Wang Xinyao , Yan Haoyun , Wei Changyin , Yan Ge , Zhao Changlei , Liu Gongzhen , Liu Xin 
Published: February 2021

Abstract
Background: Schistosomiasis is an important zoonotic parasitic disease that is widely prevalent in tropical and subtropical countries and regions in the worldwide.Methods: We aimed to analyze the proteomic differences between adult S. japonicum worms in Weishan Lake of Shandong province and the Jiangsu Yangtse river. Isobaric tags for relative and absolute quantification (iTRAQ) assays were used to analyze the differential proteomic profiles between female and male adult worms.Results: A total of 2364 adult S. japonicum proteins were identified, and 1901 proteins were quantified by isobaric tags for relative and absolute quantification (iTRAQ) technology. Our results revealed 68 differentially expressed proteins (DEPs) in female adult worms and 55 DEPs in male adult worms. LC-MS/MS and bioinformatics analysis indicated that these DEPs are enriched in cellular composition, molecular function, biological function and catabolism pathways. Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), and Domain and Clusters of Orthologous Groups (COG) analyses indicated that several groups of DEPs were involved in regulating transport, metabolism, signal transduction, energy production and conversion, defense and biosynthesis in adult S. japonicum worms. Our findings indicated that adult S. japonicum worms derived from O. hupensis that were transferred from permissive to nonpermissive areas exhibited moderate changes at the proteomic level. Moreover, snails transferred to the Weishan Lake did not change their schistosomiasis transmission ability and remained pathogenic in mice. In addition, three upregulated proteins (peptidylprolyl isomerase, heat shock protein 90α and receptor expression-enhancing protein (Q5DBJ1)) and three downregulated proteins (histone H3, histone H4 and receptor expression-enhancing protein (C1L9D7)) were found in both female and male adult worms. Conclusions: Proteomic analysis showed that differentially expressed proteins (DEPs) between adult S. japonicum worms in Weishan Lake of Shandong province and the Jiangsu Yangtse river. The results of this proteomics analysis of adult worms that hatched in two separate intermediate hosts help to improve our understanding of the growth and developmental mechanisms of S. japonicum in different environments. Under the South-to-North Water Diversion Project (SNWDP) framework, long-term surveillance is needed to prevent the diffusion of O. hupensis and to reduce the risk of schistosomiasis transmission.
Cell type classification and discovery across diseases, technologies and tissues reveals conserved gene signatures and enables standardized single-cell readouts
Authors: Savova Virginia , Chamberlain Mathew , Hanamsagar Richa , Nestle Frank , Rinaldis Emanuele de 
Published: February 2021

Abstract
Autoimmune diseases are a major cause of mortality1,2. Current treatments often yield severe insult to host tissue. It is hypothesized that improved therapies will target pathogenic cells selectively and thus reduce or eliminate severe side effects, and potentially induce robust immune tolerance3. However, it remains challenging to systematically identify which cellular phenotypes are present in cellular ensembles. Here, we present a novel machine learning approach, Signac, which uses neural networks trained with flow-sorted gene expression data to classify cellular phenotypes in single cell RNA-sequencing data. We demonstrate that Signac accurately classified single cell RNA-sequencing data across diseases, technologies, species and tissues. Then we applied Signac to identify known and novel immune-relevant candidate drug targets (n = 12) in rheumatoid arthritis. A full release of this workflow can be found at our GitHub repository (https://github.com/mathewchamberlain/Signac).
Additive Interaction Between Potentially Modifiable Risk Factors And Ethnicity In The Han, Tujia and Miao Populations With First-Ever Ischemic Stroke
Authors: Zhang Na , Wu Xinrui , Tian Mengyuan , Wang Xiaolei , Ding Jian , Tian Yong , Liang Chengcai , Zeng Zhi , Xiang Hua , Tan Hongzhuan 
Published: February 2021

Abstract
Background: As a country with one-fifth of the global population, China has had explosive growth in ischemic stroke burden with significant ethnic and geographic disparities. The aim of this study was to examine the relative risk of potentially modifiable risk factors for ischemic stroke among the Han population and two ethnic minorities (Tujia and Miao).Methods: A case-control study was conducted: 324 cases of first-ever IS from the hospitals of the Xiangxi Tujia and Miao Autonomous Prefecture and 394 controls were surveyed using structured questionnaires from communities covering the same area. Univariate and multivariate logistic regression analyses with adjusted odds ratios (ORs) and 95% confidence intervals (CIs) were used to examine the association between risk factors and ischemic stroke. The additive model was used to study the interaction between the modifiable risk factor and ethnicity in the R software.Results: Higher high-sensitivity C-reactive protein level(OR 50.54, 95%CI 29.76-85.85), higher monthly family income(4.18, 2.40-7.28), increased frequency of hot pot consumption (2.90, 1.21-6.93), diabetes mellitus (2.62, 1.48-4.62), higher apolipoprotein(Apo)B/ApoA1 ratio(2.60, 1.39-4.85), hypertension(2.52, 1.45-4.40) and moderate-intensity physical activity(0.50, 0.28-0.89) were associated with ischemic stroke. There is an additive interaction between ApoB/ApoA1 ratio and ethnicity in Tujia and Miao populations with first-ever ischemic stroke (the relative excess risk due to interaction was 5.75, 95%CI 0.58~10.92; the attributable proportion due to interaction was 0.65, 95%CI 0.38~0.91; the synergy was 3.66, 95%CI 1.35~9.93). Conclusions: It is the first case-control study examining modifiable risk factors for ischemic stroke among the Han population and two ethnic minorities (Tujia and Miao) in China, some differences were observed in the impact of risk factors among these ethnic groups. Our results may help interpret health-related data, including surveillance and research, when developing strategies for stroke prevention.
Tenascin-c Knockdown Suppresses Vasculogenic Mimicry of Gastric Cancer by Inhibiting ERK- Triggered EMT
Authors: Kang Xing , xu en , wang Xingzhou , Qian Lulu , Yang Zhi , Yu Heng , Wang Chao , Ren Chuanfu , Lu Xiaofeng , Xia Xuefeng , Guan Wenxian , Qiao Tong 
Published: February 2021

Abstract
BackgroundGastric cancer is one of the most common malignancies worldwide and vasculogenic mimicry (VM) is considered to be the leading cause for the failure of anti-angiogenesis therapy in advanced gastric cancer patients. Tenascin-c (TNC) plays a pivotal role in VM. Thus, we explored the role of TNC in VM formation in gastric cancer.MethodsGastric cancer tissues and corresponding adjacent tissues were collected from gastric cancer patients after surgery. We used western blot and immunohistochemistry to examine the expression of TNC in tissues and used siRNA and lentivirus to knockdown the TNC expression in gastric cancer cell lines. Then three-dimensional culturing, CCK-8, Edu assay, flow cytometry, trasnwell and pseudopodia formation assay were used to evaluate the function of TNC in gastric cancer cells and bioinformatic prediction was used to explore the mechanism underlying TNC modulating the VM in gastric cancer. Xenograft and peritoneal dissemination model were used to further explore the role of TNC in vivo.ResultsIn this study, we demonstrated that TNC was highly expressed in gastric cancer tissues and correlated with poor prognosis of gastric cancer. Furthermore, knockdown of TNC significantly inhibited VM formation and proliferation of gastric cancer cells in vitro and in vivo, with a reduction in cell migration and invasion. Mechanistically, TNC knockdown suppressed the phosphorylation of ERK and subsequently inhibited the process of EMT, both of which play an important role in VM formation. What’s more, rescue experiments showed that activation of p-ERK could reverse the suppressive role of TNC knockdown in gastric cancer cells.ConclusionsTNC plays an important role in VM formation in gastric cancer. Combining inhibition of TNC and ERK may be a potential therapeutic approach to inhibit gastric cancer growth and metastasis and decrease anti-angiogenic therapeutic resistance.
Fertility Treatments and the Risk of Preterm Birth Among Women with Subfertility: A Linked-Data Retrospective Cohort Study
Authors: Sanders Jessica N , Simonsen Sara E , Porucznik Christina A , Hammoud Ahmad O , Smith Ken , Stanford Joseph 
Published: February 2021

Abstract
Background: In vitro fertilization (IVF) births contribute to a considerable proportion of preterm birth (PTB) each year. However, there is no formal surveillance of adverse perinatal outcomes for less invasive fertility treatments. The study objective was to determine the effect of fertility treatment (in vitro fertilization, intrauterine insemination, usually with ovulation drugs (IUI), or ovulation drugs alone) on preterm birth, compared to no treatment in subfertile women.Methods: The Fertility Experiences Study (FES) is a retrospective cohort study conducted at the University of Utah between April 2010 and September 2012. Women with a history of primary subfertility self-reported treatment data via survey and interviews. Participant data were linked to birth certificates and fetal death records to asses for perinatal outcomes, particularly preterm birth.Results: A total 487 birth certificates and 3 fetal death records were linked as first births for study participants who completed questionnaires. Among linked births, 19% had a PTB. After adjustment for maternal age, paternal age, maternal education, annual income, religious affiliation, female or male fertility diagnosis, and duration of subfertility, the odds ratios and 95% confidence intervals (CI) for PTB were 2.17 (CI: 0.99, 4.75) for births conceived using ovulation drugs, 3.17 (CI: 1.4, 7.19) for neonates conceived using IUI and 4.24 (CI: 2.05, 8.77) for neonates conceived by IVF, compared to women with subfertility who used no treatment during the month of conception. A reported diagnosis of female factor infertility increased the adjusted odds of having a PTB 2.99 (CI: 1.5, 5.97). Duration of pregnancy attempt was not independently associated with PTB. In restricting analyses to singleton gestation, odds ratios remained elevated but were not significant for any type of treatment.Conclusion: IVF, IUI, and ovulation drugs were all associated with a higher incidence of preterm birth and low birth weight, predominantly related to multiple gestation births.
Redox-Active Electrolyte based MnWO4//AC Asymmetric Supercapacitors
Authors: Donolikar Pratiksha , Patil Seema , Sadale Shivaji , Ryu Jungho , Patil Deepak 
Published: February 2021

Abstract
Finding supercapacitive materials with high energy and power densities has attracted significant interest in recent years. Herein, we are reporting layered MnWO4 nanostructure for supercapacitor applications. MnWO4//AC asymmetric cell was fabricated by using hydrothermally synthesized MnWO4 nanostructure as a cathode and activated carbon as an anode. Prior to device fabrication, the structural and electrochemical properties of MnWO4 were thoroughly studied. MnWO4//AC asymmetric cell with KOH electrolyte showed specific capacitance and energy density of 90 F/g (at 1 mA/cm2) and 51 Wh/kg, respectively. Upon addition of redox-active KI into KOH, both the specific capacitance and energy density were significantly enhanced (144 F/g and 90 Wh/Kg, respectively). The enhanced electrochemical properties of MnWO4//AC asymmetric cell can be attributed to the high-speed solution-phase Faradic reactions contributed by KI redox species in the KOH electrolyte.
Transfer Method of Geometric Tolerance Items Based on Assembly Joints
Authors: MA Ning , YANG Bo , LI Jinping , LIU Yanchao , WANG Dianxi , GAO Changqing 
Published: February 2021

Abstract
In order to reduce the uncertainty in the selection of geometric tolerance items, a qualitative method for top-down transfer of geometric tolerance items based on assembly joints is proposed. According to the structural characteristics, the assembly joints are divided into meta-assembly joints and composite assembly joints, and the priority rules for assembly joints are proposed. The transfer path of part-level geometric tolerance items is established according to the functional requirements and structural constraints among parts. On this basis, by adding information about the composition and constraint types of assembly joints between parts and the positioning constraint relationship of the general structure surface in the part, the transfer path of part-level geometric tolerance items is extended to the surface-level transfer path. The structural transformation rules for functional requirements based on structural constraints, the tolerance item generation specifications and datum transfer specifications are established. And based on the above specifications, the mapping relationship between functional requirements, structural constraints and geometric tolerance items is defined. The synchronous transmission of geometric tolerance items along with the product design process are realized which provides an effective analysis tool for the top-down design of geometric tolerance items. Finally, the effectiveness of the method is verified by taking the transmission parts and connection parts in the crankshaft-piston mechanism as an example.
Short Daytime Napping Reduces the Risk of Cognitive Decline in Community-Dwelling Elderly Individuals: a 5-Year Longitudinal Study
Authors: Kitamura Kaori , Watanabe Yumi , Nakamura Kazutoshi , Takano Chikako , Hayashi Naomi , Sato Hisami , Someya Toshiyuki 
Published: February 2021

Abstract
Background: Beneficial effects of napping on cognition have been suggested in cross-sectional studies. This study aimed to clarify longitudinal associations between cognitive decline and sleep characteristics, particularly daytime napping, over a 5-year period in older adults. Methods: Study participants were 389 community-dwelling individuals aged ≥65 years living in Ojiya City, Niigata, Japan. Baseline and follow-up examinations were conducted in 2011-2013 and 2016-2018, respectively. Trained nurses visited and interviewed participants to collect the following information: demographic characteristics, disease history, lifestyle habits including sleep and daytime napping, and cognitive function at baseline; and cognitive function at follow-up. The assessment of cognitive function was performed using the revised Hasegawa’s dementia scale (HDS-R), with cognitive decline defined as a 5-year change in HDS-R of ≤–3. Odds ratios (ORs) for cognitive decline were calculated using multiple logistic regression analysis.Results: Mean age of participants was 74.6 years (SD 6.4), and the cumulative incidence of cognitive decline was 106/389 (27.3%). The multivariable-adjusted OR for 1-29 min daytime napping was significantly lower compared to that for no napping (OR=0.47, 95%CI: 0.23-0.96). Bedtime was inversely associated with cognitive decline (multivariable-adjusted P for trend=0.0480).Conclusion: Short daytime napping (<30 min) reduces the risk of 5-year cognitive decline in community-dwelling older people. A future study will be necessary to confirm the effect of short napping on the reduction of risk for clinically diagnosed dementia.
Soil causes gut microbiota to flourish and total serum IgE levels to decrease in mice
Authors: Zhou Dongrui , Li Na , Yang Fan , Zhang Honglin , Bai Zhimao , Dong Yangyang , Li Mengjie , Zhu Wenyong , Fei Zhongjie , Xiao Pengfeng , Sun Xiao , Lu Zuhong 
Published: February 2021

Abstract
Background
Traditional farm environments provide protection from allergic diseases. In this study, farm environmental factors were classified into three categories: environmental microbes, soil, and organic matter. To explore the impact of soil and environmental microorganisms on gut microbiota and immune function, mice were fed sterilized soil, soil microbes (in lieu of environmental microbes), or non-sterilized soil.
Results
Metagenomic sequencing results showed that the intake of sterile soil while inhaling a small amount of soil microbes in the air, increased gut microbial diversity and the abundance of type III secretion system (T3SS) genes and decreased total serum IgE levels induced by 2-4-dinitrofluorobenzene. The intake of soil microbes increased the abundance of genes involved in the metabolism of short-chain fatty acids and amino acid biosynthesis. By contrast, the intake of soil increased gut microbial diversity, the abundance of T3SS genes and related infectious elements, and genes associated with the metabolism of short-chain fatty acids and amino acid biosynthesis and decreased serum IgE levels. The immune function was positively and significantly correlated with the bacterial secretion system genes, especially with that of T3SS.
Conclusions
An important mechanism through which farm environments exert a protective effect against allergic diseases could be by serving as a “prebiotic” promoting the reproduction and growth of some intestinal microorganisms that harbor bacterial secretion system genes, especially those of T3SS, whose abundance was positively and significantly correlated with innate immune function of mice.
Osseous tools and personal ornaments from the Epigravettian sequence at Badanj
Authors: Borić Dušan , Cristiani Emanuela , Pravidur Andrijana , Marić Ana , Whallon Robert 
Published: February 2021

Abstract
The Late Upper Palaeolithic (Epigravettian) sequence at Badanj has yielded an important dataset about the occupation of the hinterland of the Eastern Adriatic catchment zone in the late Pleniglacial. The site also harbors one of the rare occurrences of Upper Palaeolithic parietal “art” in southeastern Europe in the form of a large rock engraving. Another notable aspect of the site is the presence of engravings on portable objects made from bone. The first excavations at Badanj, conducted in 1976–1979 in the zone around the engraved rock, yielded a surprisingly large number of personal ornaments (over 1000 specimens) from a variety of primarily marine gastropods, scaphopods, and bivalves, and red deer canines. Here we review what is currently known about the site and report our preliminary findings from the study of the collection of personal ornaments as well as osseous tools, some of which were marked by regular incisions forming decorative motifs. We also report two new direct accelerator mass spectrometry (AMS) dates on antler barbed points.
Mibefradil inhibits the proliferation of triple-negative breast cancer by targeting AURKA to regulate apoptosis signal pathway
Authors: Han Xu , Qu Xiujuan , Liu Beixing , Wang Yizhe , Cheng Yang , Lu Wenqing , He Xin , Tang Shiying , Liu Yunpeng , Che Xiaofang , Teng Yuee 
Published: February 2021

Abstract
Background: Triple negative breast cancer (TNBC) is a tumor characterized by high recurrence and mortality, but without effective targeted therapy. It is urgent to explore new treatment strategy to improve the efficacy of TNBC therapy. Methods: Transcriptomic profiling datasets of TNBC were used for screening TNBC specific gene sets. Drug prediction was performed in Connectivity map (CMap) database. Molecular docking method was used for analyzing drug targets. In vitro and in vivo models of TNBC were constructed to examine the drug efficacy. Results: We screened out Mibefradil, a T-type Ca2+ channel blocker, might be a potential therapeutic drug for TNBC by transcriptomics and bioinformatics analysis, and verified that Mibefradil could inhibit the proliferation of TNBC cells by inducing apoptosis and cell cycle arrest. Furthermore, by network pharmacology and molecular docking analysis, AURKA was predicted as the most possible drug target of Mibefradil. Finally, it was proved that Mibefradil treatment could induce apoptosis by decreasing protein expression and phosphorylation level of AURKA in vitro and in vivo. Conclusions: Mibefradil played anti-cancer role in TNBC cells by targeting to AURKA to induce cell cycle and apoptosis. Our results repurposed Mibefradil as a potential targeted drug of TNBC and provided a fundamental research for a novel strategy TNBC treatment.
Experimental and numerical investigation of the effect of holes on the springback in cold roll forming of UHSS
Authors: Liu Zhi-min , Zhang Pan , liu xiaoli , zhang ming , ma qiang , huang suxia , wang yanhui , zhao rongguo 
Published: February 2021

Abstract
Ultra-high strength steel (UHSS) pre-notched sections are getting growing popularity in the automotive industry with the development of automotive lightweight. However, the springback of UHSS products is large, and the existence of holes also has an effect on the springback. Accurate prediction of springback of UHSS pre-notched products in cold roll forming ( CRF ) is a key issue to be solved. In this paper, the effect of holes on the springback of UHSS in CRF is discussed by simulation and experiment. The finite element model of pre-notched car threshold was constructed, and its accuracy was validated by continuous CRF experiment. The mathematical model of variable elastic modulus determined by tensile tests of martensite (MS) 1300 was applied in finite element model. The accuracy of springback was improved by 15% in the hole region by using variable elastic modulus . Several forming schemes were designed to research the effect of different features on the springback in the hole region. The results show that the existence of holes reduces the springback and the effect is different at different positions of the car threshold. The springback in the hole region decreases with the increase of the number of stands, the strip thickness and the hole diameter, and with the decrease of the distance between stands and the distance between holes. This study provides a help for reducing the influence of holes on the springback and improving the forming precision of pre-notched sections in the actual production of CRF.
Fixed-Time Bounded H Infinity Tracking Control of a Single-Joint Manipulator System with Input Saturation
Authors: chen ming 
Published: February 2021

Abstract
Based on Lyapunov finite-time stability theory and backstepping strategy, we put forward a novel fixed-time bounded H infinity tracking control scheme for a single-joint manipulator system with input saturation. The main control objective is to maintain that the system output variable tracks the desired signal at fixed time. The advantages of this paper are the settling time of the tracking error converging to the origin is independent of the initial conditions, and its convergence speed is more faster. Meanwhile, bounded H infinity control is adopted to suppress the influence of the external disturbances on the controlled system. At the same time, the problem of input saturation control is considered, which effectively reduce the input energy consumption. Theoretical analysis shows that the tracking error of the closed-loop system converges to a small neighborhood of the origin within fixed time. In the end, a simulation example is presented to demonstrate the effectiveness of the proposed scheme.