bluuebunny/crossref_metadata_2025_split huggingface dataset:
Therapeutic STING activation boosts microglia phagocytosis and ameliorates depression-like behaviors during chronic restraint stress
Authors: Zhai Qian , Zhang Yanpeng , Tan Shuwen , Sun Jianyu , Ye Mao , Gao Hui , Pu Kairui , Wu Meiyan , Wang Qiang 
Published: July 2021

Abstract
Background
The STING-TBK1-IRF3 signaling pathway involves in modulating host innate immunity, however, the potential role of STING signaling pathway in chronic restraint stress model has not been determined. The aim of this study is to explore the underlying role of STING signaling pathway in regulating neuroinflammation, as well as to evaluate the therapeutic potential of STING agonist during chronic restraint stress.
Methods
C57BL/6 mice were subject to 14-day intermittent restraint stress. Sucrose preference, elevated plus maze and tail suspension tests were measured in chronic restraint stress mice. Expression levels of proinflammatory cytokines were tested by QT-PCR and Luminex cytokine assays. The fluorescence-labeled latex beads, flow cytometry and CD68 positive cell counts were utilized to evaluate phagocytic abilities of microglia. Then, the ability of intracerebroventricular injection of STING agonist, 2’3-cGAMP, to reverse the depression-like behaviors and inflammatory cytokines was examined.
Results
We found that the expression levels of STING, p-TBK1, and p-IRF3 were remarkably decreased in chronic restraint stress mice, which was associated with decreased IFN-β secretion. Moreover, the STING agonist, 2’3-cGAMP, significantly alleviated the neuroinflammation and ameliorated depression-like behavior which depends on the functional STING activation. Furthermore, 2’3-cGAMP promoted microglia phagocytosis through cGAMP-STING-dependent IFN-β release, which was essential for recovery from neuroinflammation during chronic restraint stress.
Conclusions
These findings demonstrate that STING signaling pathway is a critical mediator in regulating microglia phagocytosis and may serve as a novel therapeutic target for chronic stress-related psychiatric diseases.
Prevalence and Predictors of Overweight and Obesity among Military Personnel with Low Socio-Economic Status in Kuala Lumpur
Authors: Rahim Nik Qistina Nik Abd , Zulkefli Nor Afiah Mohd , Moideen Mohd Arshil 
Published: July 2021

Abstract
Background: It is a major public health concern when 52% of adults worldwide were overweight and obese. Military institutions worldwide were included, which led to negative impacts on the overall combat readiness due to the obesity-associated-diseases. This study determined the prevalence and predictors of overweight and obesity among the low socioeconomic status (SES) army personnel in Kuala Lumpur. Methods: A cross-sectional study involving 772 low SES army personnel in Kuala Lumpur was conducted. Online questionnaires were distributed while anthropometric measurements’ data were obtained from Military Lifetime Health Record (MLHR) system with BMI of > 25kg/m2 as the outcome. Data analysed using SPSS version 23.0 (descriptive and inferential statistics). Results: The median age of the respondents was 30 (IQR=7) years. The prevalence of overweight and obese army personnel was 41.1% (95%CI:37.6-44.6). The predictors found were being married (AOR:2.026,95%CI:1.318-3.113), secondary education (AOR:2.545,95%CI:1.245-5.203), Lance Corporal (AOR:1.994,95%CI:1.061-3.748), Corporal (AOR:2.814,95%CI:1.578-5.020), Sergeant (AOR:4.174,95%CI:2.076-8.390), past injury (AOR:1.879,95%CI: 1.191-2.965), history of obese sibling (AOR:1.737,95%CI:1.013-2.973), history of obese parent (AOR:3.344,95%CI:1.965-5.688), history of obese grandparent (AOR:11.321,95%CI:2.207-58.072), poor knowledge on dietary intake (AOR:1.524,95%CI:1.077-2.157), less than 2 litres daily plain water intake (AOR:1.606,95%CI:1.166-2.210). Conclusions: Knowledge on dietary intake and plain water intake are the two modifiable predictors for overweight and obesity found in this study, while the other nine predictors are non-modifiable. Understanding on issues surrounding the low SES group will help the strategic level in planning for future comprehensive interventions on overweight and obesity, specifically targeting on those predictors.
Aggregation Pheromone of the Guam Strain of Coconut Rhinoceros Beetle, Oryctes Rhinoceros, and Revision of Stereochemistry
Authors: Hall David R , Harte Steven J , Farman Dudley I , Ero Mark , Pokana Alfred 
Published: July 2021

Abstract
The coconut rhinoceros beetle, Oryctes rhinoceros (Linnaeus 1758) (Coleoptera: Scarabaeidae: Dynastinae) (CRB), is endemic to tropical Asia where it damages both coconut and oil palm. A new invasion by CRB occurred on Guam in 2007 and eradication attempts failed using commonly applied O. rhinoceros nudivirus (OrNV) isolates. This and subsequent invasive outbreaks were found to have been caused by a previously unrecognized haplotype, CRB-G, which appeared to be tolerant to OrNV. The male-produced aggregation pheromone of the endemic, susceptible strain of O. rhinoceros (CRB-S) was previously identified as ethyl 4-methyloctanoate. There were anecdotal reports that the commercial lures containing this compound were not attractive to CRB-G and the aim of this work was to identify the pheromone of CRB-G. Initial collections of volatiles from virgin male and female CRB-G adults failed to show any male- or female-specific compounds as candidate pheromone components. Only after five months were significant quantities of ethyl 4-methyloctanoate and 4-methyloctanoic acid produced by males but not by females. No other male-specific compounds could be detected, in particular methyl 4-methyloctanoate, 4-methyl-1-octanol or 4-methyl-1-octyl acetate, compounds identified in volatiles from some other species of Oryctes. Ethyl 4-methyloctanoate elicited a strong electroantennogram response from both male and female CRB-G, but these other compounds, including 4-methyloctanoic acid, did not. The enantiomers of ethyl 4-methyloctanoate and 4-methyloctanoic acid were conveniently prepared by enzymatic resolution of the commercially-available acid, and the enantiomers of the acid, but not the ester, could be separated by gas chromatography on an enantiospecific cyclodextrin phase. Using this approach, both the ethyl 4-methyloctanoate and the 4-methyloctanoic acid produced by male CRB-G were shown to be exclusively the (R)-enantiomers whereas previous reports had suggested male CRB-S produced the (S)-enantiomers. However, re-examination of the ester and acid produced by male CRB-S showed that these were also the (R)-enantiomers. In field trapping experiments, both racemic and ethyl (R)-4-methyloctanoate were highly attractive to both male and female CRB-G. The (S)-enantiomer and the corresponding acids were only weakly attractive. Addition of racemic 4-methyloctanoic acid to ethyl 4-methyloctanoate did significantly increase attractiveness, but addition of (R)- or (S)-4-methyloctanoic acid to the corresponding ethyl esters did not. Reasons for the previous misassignment of the configuration of the components of the CRB-S pheromone are discussed along with the practical implications of these results.
Abatement of Forskolin-induced Intestinal Fluid Secretion by Modulation of Intracellular Camp With Penicillin
Authors: Chen Earnest , Own Jason L. , Ollodart Jenna , Toksoy Zeren , Davis Bruce A. , Geibel John P. 
Published: July 2021

Abstract
Millions of people die every year due to diarrheal related diseases, with infants and the elderly making up the majority of these deaths. Deaths are caused by excessive intestinal fluid and electrolyte secretion and are especially common in impoverished developing countries. Antibiotics have been classically used as a method to treat diarrhea-related pathologies by modulating the gut microbiome. We recently reported that penicillin may protect against disease-induced excessive fluid and electrolyte secretion via a genetics-independent, microbiome-independent mechanism in individual colonic crypt cells. In this study we investigated whether microbial-independent protective effects of penicillin against fluid secretion can be observed in the rat small intestine at the whole-tissue level. Here we report that penicillin has a significant dose-dependent protective effect against fluid secretion in induced models of diarrhea in the microbiome deficient rat small intestine. Penicillin can rapidly bring fluid secretion down to levels comparable to healthy controls. Our results suggest, for the first time, an alternative function for penicillin G as a cost-effective and fast-acting treatment against diarrheal symptoms without dependence on modulating the behavior of the existing gut microbiome.
Polymorphism Rs34562254 In TNFRSF13B is Associated with HCV Infection Outcomes Among Chinese Han Population
Authors: Fan Haozhi , Fu Zuqiang , Ge Zhijun , Dong Chen , Wang Chunhui , Shen Chao , Li Jun , Yu Rongbin , Zhang Yun , Yue Ming 
Published: July 2021

Abstract
Genetic variations in tumor necrosis factor receptor superfamily (TNFRSF) 13B have already proved to be associated with immune- related diseases. This study aimed to explore the relationship of tumor necrosis factor superfamily (TNFSF) 13, TNFRSF13B, and TNFRSF14 missense mutations with the susceptibility of HCV infection. SNPs in TNFSF13 (rs3803800, rs11552708), TNFRSF13B (rs34562254), and TNFRSF14 (rs4870) were genotyped in 469 intravenous drug users, 728 hemodialysis patients and 1636 paid blood donors by TaqMan real-time PCR assay. And the USCS Brower and RNAfold web servers were used to predict the potential biological functions of the positive SNPs. After adjustment for gender, age, ALT level, AST level and route of infection, the results of logistic regression analysis showed that the subjects carried rs34562254 TT mutant homozygous type in TNFRSF13B were more likely to be infected by HCV compared to those with rs34562254 CC wild homozygous type (co-dominant model: OR = 1.52, 95% CI: 1.17–1.98, P = 0.002; recession model: OR = 1.41, 95% CI: 1.10–1.81, P = 0.007; additive model: OR = 1.21, 95% CI: 1.07–1.37, P = 0.002). And the effect of the risk allele (rs34562254-T) is more evident in female, elder (≥ 50), paid blood donors, lower ALT level (≤ 40 U/L) and lower ALT level (≤ 40 U/L) subgroup. The result of bioinformatics analysis showed that rs34562254 was located at the highest peak of the H3K4Me1 histone marker with the UCSC genome browser. And the minimum free energy (MFE) of the centroid secondary structure for the mutant T allele (-38.90 kcal/mol) of rs34562254 was higher than that of the wild C allele (-45.60 kcal/mol) with the RNAfold web servers. In addition, the RegulomeDB score of rs34562254 was 3a with the RegulomeDB web servers. All the above results of bioinformatics analysis suggested that rs34562254 may affect the gene expression level by regulating the transcriptional activity of the corresponding gene region. The rs34562254 polymorphisms in TNFRSF13B were significantly associated with HCV infection among Chinese Han population.
State-Selective Electron Capture Cross Sections in Collision Between Be4+ and Ground State Hydrogen Atom – Significant Improvement in the Classical Treatment
Authors: Ziaeian Iman , Tőkési Károly 
Published: July 2021

Abstract
We present state-selective electron capture cross sections in collision between Be4+ and ground state hydrogen atom. The n- and nl-selective electron capture cross sections are calculated by a three-body classical trajectory Monte Carlo method (CTMC) and by a classical simulation schema mimicking quantum features of the collision system. The quantum behavior is taken into account with the correction term in the Hamiltonian as was proposed by Kirschbaum and Wilets (C. L. Kirschbaun, and L. Wilet, Phys. Rev. A 21, 834 (1980)). Calculations are carried out in the projectile energy range of 1-1000 keV/amu. We found that our model for Be4++ H(1s) system remarkably improves the obtained state-selective electron capture cross sections, especially at lower projectile energies. Our results are very close and are in good agreement with the previously obtained quantum-mechanical results. Moreover our model with simplicity can time efficiently carry out simulations where maybe the quantum mechanical ones become complicated, therefore, our model should be an alternative way to calculate accurate cross sections and maybe can replace the quantum-mechanical methods.
Bone Metastasis is Uncommon in Patients with Oral Squamous Cell Carcinoma at Diagnosis-Bone Scan may be Not Necessary for Staging
Authors: Wang Cheng-Ping , Hsu Wan-Lun , Lou Pei-Jen , Ko Jenq-Yuh , Chen Tseng-Cheng 
Published: July 2021

Abstract
Only a small portion of the patients with oral squamous cell carcinoma (OSCC) experience bone metastasis. This study is to evaluate the percentage of bone metastasis at diagnosis and after treatment and to discuss whether bone scan is necessary in OSCC patients. The medical records of all patients with newly diagnosed OSCC receiving bone scan or positron emission tomography (PET) at diagnosis between 2010 and 2015 were retrospectively reviewed. A total of 1049 patients were enrolled, including 458 (44%) with early stage tumor and 591 (56%) with late stage tumor. In total, 33 (3.1%) patients had bone metastasis at diagnosis (9 patients, 0.86%) and after treatment (24 patients, 2.29%) during the mean follow-up of 12 months. Among those with subsequent bone metastasis, only 5 (21%) patients had bone-only metastasis. All patients with bone metastasis before primary treatment and all but two patients with bone metastasis after treatment had locoregionally advanced OSCC. The mean survival time of the 33 patients after diagnosis of bone metastasis was 121 days. Bone metastasis is uncommon in patients with OSCC. It is not necessary to routinely use bone scan for initial staging, especially early stage OSCC, and for follow-up.
Impact of Admission Hyperglycemia on Short and Long-Term Prognosis in Acute Myocardial Infarction: MINOCA versus MIOCA
Authors: Paolisso Pasquale , Foà Alberto , Bergamaschi Luca , Angeli Francesco , Fabrizio Michele , Donati Francesco , Sebastiano Toniolo , Chiara Chiti , Andrea Rinaldi , Stefanizzi Andrea , Armillotta Matteo , Sansonetti Angelo , Magnani Ilenia , Iannopollo Gianmarco , Rucci Paola , Gianni Casella , Galiè Nazzareno , Pizzi Carmine 
Published: July 2021

Abstract
BackgroundThe prognostic role of hyperglycemia in patients with myocardial infarction and obstructive coronary arteries (MIOCA) is acknowledged, while data on non-obstructive coronary arteries (MINOCA) are still lacking. Recently, we demonstrated that admission stress-hyperglycemia (aHGL) was associated with a larger infarct size and inflammatory response in MIOCA, while no differences were observed in MINOCA. We aim to investigate the impact of aHGL on short and long-term outcomes in MIOCA and MINOCA patients.MethodsMulticenter, population-based, cohort study of the prospective registry, designed to evaluate the prognostic information of patients admitted with acute myocardial infarction to S. Orsola-Malpighi and Maggiore Hospitals of Bologna metropolitan area. Among 2704 patients enrolled from 2016 to 2020, 2431 patients were classified according to the presence of aHGL (defined as admission glucose level ≥ 140mg/dL) and AMI phenotype (MIOCA/MINOCA): no-aHGL (n = 1321), aHGL (n = 877) in MIOCA and no-aHGL (n = 195), aHGL (n = 38) in MINOCA. Short-term outcomes included in-hospital death and arrhythmias. Long-term outcomes were all-cause and cardiovascular mortality.ResultsaHGL was associated with a higher in-hospital arrhythmic burden in MINOCA and MIOCA, with increased in-hospital mortality only in MIOCA. After adjusting for age, gender, hypertension, Killip class and AMI phenotypes, aHGL predicted higher in-hospital mortality in non-diabetic (HR = 4.2; 95% CI 1.9–9.5, p = 0.001) and diabetic patients (HR = 3.5, 95% CI 1.5–8.2, p = 0.003). During long-term follow-up, aHGL was associated with 2-fold increased mortality in MIOCA and a 4-fold increase in MINOCA (p = 0.032 and p = 0.016). Kaplan Meier 3-year survival of non-hyperglycemic patients was greater than in aHGL patients for both groups. No differences in survival were found between hyperglycemic MIOCA and MINOCA patients. After adjusting for age, gender, hypertension, smoking, LVEF, STEMI/NSTEMI and AMI phenotypes (MIOCA/MINOCA), aHGL predicted higher long-term mortality.ConclusionsaHGL was identified as a strong predictor of adverse short- and long-term outcomes in both MIOCA and MINOCA, regardless of diabetes. aHGL should be considered a high-risk prognostic marker in all AMI patients, independently of the underlying coronary anatomy.Trial Registrationdata were part of the ongoing observational study AMIPE: Acute Myocardial Infarction, Prognostic and Therapeutic Evaluation. ClinicalTrials.gov Identifier: NCT03883711.
Alterations to the gut microbiome after sport-related concussion in a collegiate football players cohort
Authors: Soriano Sirena , Curry Kristen , Sadrameli Saeed , Wang Qi , Nute Michael , Reeves Elizabeth , Kabir Rasadul , Wiese Jonathan , Criswell Amber , Schodrof Sarah , Britz Gavin , Gadhia Rajan , Podell Kenneth , Treangen Todd , Villapol Sonia 
Published: July 2021

Abstract
Concussions, both single and repetitive, during contact sports cause brain and body alterations in athletes. The role of the brain-gut connection and changes in the microbiota have not been well established after a head injury or concussion-related health consequences. We recruited 33 Division I Collegiate football players and collected blood, stool, and saliva samples throughout the athletic season. Analysis of the gut microbiome reveals a decrease in abundance for two bacterial species, Eubacterium rectale and Anaerostipes hadrus, after a diagnosed concussion. No significant differences were found regarding the salivary microbiome. Serum biomarker analysis shows an increase in GFAP blood levels in athletes during athletic activity. Additionally, S100β and SAA blood levels were positively correlated with the abundance of Eubacterium rectale species among athletes exposed to subconcussive impacts. These novel findings provide evidence that detecting changes in the gut microbiome may pave the way for improved concussion diagnosis following head injury.
Numerical Calculation of Grinding Wheel Wear for Spiral Groove Grinding
Authors: Liu Xianli , Wang Shipeng , Yue Caixu , Xu Mengdi , Chen Zhan , Zhou Jiaqi , Liang Steven Y. 
Published: July 2021

Abstract
Due to its good cutting performance in titanium alloy machining, integral end mills are more and more used in machining aero-engine impeller blades. The tool spiral groove plays the role of chip acceptor and chip removal, and the accuracy of its parameters has an important effect on the cutting performance. In the grinding process of the spiral groove, the grinding wheel's external grinding is mainly involved in the grinding task. The grinding wheel's wear degree is related to the grinding time and grinding times of the grinding wheel, and the wear of the grinding wheel will lead to the change of the parameters of the spiral groove. To achieve the accurate solution of the grinding wheel surface wear profile, image processing technology was used to extract the spiral groove end section contour coordinates of the grinding wheel and fit them. The worn sand profile was solved based on the contact line principle, and the grinding wheel wear amount was obtained. The traditional reconstruction method was used to verify the algorithm. The results show that the accuracy of the reverse algorithm for the wear profile of the grinding wheel is relatively high.
Complete Genome Sequence of Two Isolates of Spiraea Yellow Leafspot Virus (Genus: Badnavirus) From Spiraea x Bumalda ‘Anthony Waterer’
Authors: Alvarez-Quinto Robert Alexander , Serrano Joana , Olszewski Neil E , Grinstead Samuel , Mollov Dimitre , Lockhart Ben E 
Published: July 2021

Abstract
Isolates of spiraea yellow leafspot virus (SYLSV) were collected from Spiraea (Spiraea x bumalda) ‘Anthony Waterer’ plants showing virus-like symptoms including yellow spotting and leaf deformation in Minnesota and Maryland. The complete genome sequence of SYLSV-MN (Minnesota) and SYLSV-MD (Maryland) was 8,017bp in length. The sequences share 95% of identity at the nucleotide level. Both isolates have the same genome organization containing three open reading frames (ORFs), with ORF3 being the largest encoding the putative polyprotein of 232 kDa with conserved domains including Zinc finger, pepsin-like aspartate protease, reverse transcriptase (RT), and RNase H. Pairwise comparisons between members of the genus badnavirus showed that Gooseberry vein banding associated virus GB1 (HQ852248) and Rubus yellow net virus isolate Baumforth's Seedling A (KM078034) were the closest related virus sequences to SYLSV sharing 73% of identity at the nucleotide level. Bacilliform virions with dimensions of 150 nm x 30 nm were observed in virus preparations from symptomatic but not in asymptomatic plants.
Declining Neighborhood Simulated Annealing Algorithm to Robust Fixture Synthesis in a Point-Set Domain
Authors: Alshameri Taqiaden , Dong Yude , Alqadhi Abdullah 
Published: July 2021

Abstract
Fixture synthesis addresses the problem of fixture-elements placement on the workpiece surfaces. This article presents a novel variant of the Simulated Annealing (SA) algorithm called Declining Neighborhood Simulated Annealing (DNSA) specifically developed for the problem of fixture synthesis. The objective is to minimize measurement errors in the machined features induced by the misalignment at workpiece-locator contact points. The algorithm systematically evaluates different fixture layouts to reach a sufficient approximation of the global optimum robust layout. For each iteration, a set of previously accepted candidates are exploited to predict the next move. Throughout the progress of the algorithm, the search space is reduced and the new candidates are designated according to a declining Probability Density Function (PDF). To assure best performance, the DNSA parameters are configured using the Technique for Order Preference by Similarity to Ideal Solution (TOPOSIS). Moreover, the parameters are set to auto-adapt the complexity of a given input based on a Shanon entropy index. The optimization process is carried out automatically in the Computer-Aided Design (CAD) environment NX; a computer code was developed for this purpose using the Application Programming Interface (API) NXOpen. Benchmark examples from industrial partner and literature demonstrate satisfactory results.
A simple method to evaluate the seismic/infrasonic energy partitioning during an eruption using data contaminated by air-to-ground signals
Authors: Ichihara Mie , Yamakawa Kazuya , Muramatsu Dan 
Published: July 2021

Abstract
A volcanic eruption transmits both seismic waves and infrasound signals. The seismo-acoustic power ratio is widely used to investigate the eruption behaviors and the source dynamics. It is often the case that seismic data during an eruption are significantly contaminated or even dominated by ground shaking due to infrasound (air-to-ground signals). To evaluate the contribution of infrasound-originated power in the seismic data, we need a response function of the seismic station to infrasound. It is rare to obtain a seismo-acoustic data-set containing only infrasound signals, though it is ideal for calculating the response function. This study proposes a simple way to calculate the response function using seismo-acoustic data containing infrasound and independent seismic waves. The method requires data recorded at a single station and mainly uses the cross-correlation function between the infrasound data and the Hilbert transform of the seismic data. It is tested with data recorded by a station at Kirishima volcano, Japan, of which response function has been constrained. It is shown that the method calculates a proper response function even when the seismic data contain more significant seismic power (or noise) than the air-to-ground signals. The proposed method will be useful in monitoring and understanding eruption behaviors using seismo-acoustic observations.