bluuebunny/crossref_metadata_2025_split huggingface dataset:
Construction of a plasmid addiction system using grpE as selection marker in Escherichia coli and its application in phloroglucinol biosynthesis
Authors: Wang Ji-ming , Men Xiao , Cao Yu-jin , Zhang Hai-bo 
Published: October 2021

Abstract
Microbial synthesis of commodity chemicals often be conducted in recombinant plasmid-based expression systems, in which plasmids play pivotal roles on productivity. The recombinant plasmids always encounter instability, leading to losses in product recovery of entire process. To maintain the stability of plasmids, several mechanisms have been evolved. Plasmid addition system, selectively killing plasmid-free cells, is regard as a useful strategy to improve the proportion of plasmid-containing cells. In this study, a novel plasmid addition system using an essential gene grpE that encodes a molecular cochaperone as selection marker, avoiding use of antibiotics, was constructed in Escherichia coli . The solo copy of grpE gene on the chromosome was knocked out and relocated on multicopy plasmids. The generated strains can maintain high ratio of plasmid-harboring cells without antibiotics supplementation in mineral salts media and exhibit improved cell growth and increased tolerance to phloroglucinol. Using this system in phloroglucinol synthesis, it could significantly increase the phloroglucinol titer from 0.75 g/L to 1.26 g/L, which was further increased to 1.78 g/L when biotin-[acetyl-CoA-carboxylase] ligase BirA was overexpressed. It can be expected that this system will be a powerful tool for microbial manufacture of important chemicals in E . coli .
Evaluation of Visual and Cytology Screening in Preventing Cervical Intraepithelial Neoplasia and Cervical Cancer in Namibia
Authors: Pick Riana , Kouame Koffi , Moyo Sylvester Rodgers , Aboua Yapo Guillaume 
Published: October 2021

Abstract
An estimated 851 340 women in Namibia are at risk of human papilloma viral (HPV) infection that may cause cervical cancer. Visual screening with acetic acid (VIA) and cryotherapy have been proven highly effective. However, data associating with the impact of cervical cancer screening programmes lack. This study evaluated visual and cytology screening in preventing cervical intraepithelial lesions (CIN) and cervical cancer in Namibia. Findings showed that women with HPV, cervicitis, koilocytosis and atypia are at an increased risk of testing positive for VIA. Older women, particularly with HIV are more likely to be diagnosed with squamous cell carcinoma (SCC). VIA procedure seems to be viable in screening, although colposcopy in patient follow-up in the Namibian context should be revised and used with positive HR-HPV results. Screening modalities incorporating HPV testing for different ages and risk populations (e.g. HIV positive, autoimmune disease) is recommended. Screening uptake can further be improved by interventions that adopt a model which uses previous diagnostic outcomes, women’s health objectives, up-to-date knowledge and practice relevant to national context.
Universe: the dynamics of the minimum and maximum expansion states
Authors: Petrakis Sócrates 
Published: October 2021

Abstract
Two hypotheses stand out in describing the evolution of the Universe. The predominant one predicts that the current expansion began at a certain instant and will not preserve any variation of energy that performs work; apparent flat Universe (Ω = 1) is advocated by relativistic calculations and observational data, with an end or thermal death at its maximum expansion (3D Space). The other hypothesis considers that the Universe is cyclical (always alternating phases of expansion and contraction). This proposal aims to demonstrate that both hypotheses can be correct by not being distinct, but complementary. Supported by the immutability of physical laws, analyses of concepts such as space, mass, energy, gravity, spin, and entropy define an exclusive presence of 1D Space in the minimum and maximum expansion states of the Universe. With our 3D Space Universe created and existing between these extreme states, every dynamic is outlined and completes the usual relativity. The concept of complete rest energy (1D Space) was able to be applied, demonstrating that the complete evolution of the Universe is spatially dynamic in a perpetual time dimension, always recreating our Universe.
A pragmatic evaluation of a Public Health Knowledge Broker mentoring education program: A convergent mixed methods study
Authors: Clark Emily C , Dhaliwal Bandna , Ciliska Donna , Neil-Sztramko Sarah , Steinberg Marla , Dobbins Maureen 
Published: October 2021

Abstract
Background: Public health professionals are expected to use the best available research and contextual evidence to inform decision making. The National Collaborating Centre for Methods and Tools developed, implemented, and evaluated a Knowledge Broker mentoring program aimed at facilitating organization-wide evidence-informed decision making in ten public health units in Ontario, Canada. The purpose of this study was to pragmatically assess the impact of the program.Methods: A convergent mixed methods design was used to interpret quantitative results in the context of the qualitative findings. Quantitatively, participants’ knowledge and skills for finding, interpreting, and using evidence were measured before and after program completion via multiple-choice tests. Changes in scores were assessed using paired t-tests. Qualitatively, program participants and management at enrolled public health units were interviewed to explore the effect of program participation. A secondary analysis of these interviews was conducted to determine whether organizations met their evidence use goals set at baseline, and to identify key factors related to implementation of EIDM within the organization.Results: Post-program scores for knowledge and skills for EIDM were higher compared to pre-program scores (mean difference = 14.0%, 95% CI 8.2%, 19.8%). Organizations met their goals for evidence use to varying degrees. Key themes identified that support an organizational shift to EIDM include definitive plans for participants to share knowledge during and after program completion, embedding evidence into decision making processes, and supportive leadership with organizational investment of time and resources. The location, setting or size of health units was not associated with attainment of EIDM goals; small, rural health units were not at a disadvantage compared to larger, urban health units.Conclusions: The Knowledge Broker mentoring program effectively increased participants’ knowledge and skill, allowing them to share their learning and support change at their health units. When paired with organizational supports such as supportive leadership and resource investment, this program holds promise as an innovative knowledge translation strategy for organization wide EIDM among public health organizations.
Identification of Novel PHD-Finger Genes in Pepper by Genomic Re-Annotation and Comparative Analyses
Authors: Guk Ji Yoon , Jang Min Jeong , Kim Seungill 
Published: October 2021

Abstract
BackgroundThe plant homeodomain (PHD)-finger gene family that belongs to zinc-finger genes, plays important roles in epigenetics by regulating gene expression in eukaryotes. However, inaccurate annotation of PHD-finger genes hinders further downstream comparative, evolutionary, and functional studies.ResultsWe performed genome-wide re-annotation in Arabidopsis, rice, pepper, potato, and tomato to better understand the role of PHD-finger genes in these species. Our investigation identified 875 PHD-finger genes, of which 225 (26% of total) were newly identified, including 57 (54%) novel PHD-finger genes in pepper. The PHD-finger genes of the five plant species have various integrated domains that may be responsible for the diversification of structures and functions of these genes. Evolutionary analyses suggest that PHD-finger genes were expanded recently by lineage-specific duplication, especially in pepper and potato, resulting in diverse repertoires of PHD-finger genes among the species. We validated the expression of six newly identified PHD-finger genes in pepper with qRT-PCR. Transcriptome analyses suggest potential functions of PHD-finger genes in response to various abiotic stresses in pepper.ConclusionsOur data, including the updated annotation of PHD-finger genes, provide useful information for further evolutionary and functional analyses to better understand the roles of the PHD-finger gene family in pepper.
The correlation between fractional amplitude of low-frequency fluctuation-based resting-state functional magnetic resonance imaging and facial emotion recognition ability in patients with first-episode schizophrenia
Authors: Kuang Qijie , Liu Yi , Zhou Sumiao , Bi Taiyong , Mi Lin , She Shenglin , Zheng Yingjun 
Published: October 2021

Abstract
Our aim was to analyse the correlation between the fractional amplitude of low-frequency fluctuation (fALFF) and facial emotion recognition (FER) ability in patients with first-episode schizophrenia (FSZ). A total of 28 patients with FSZ and 33 healthy controls (HCs) completed visual search tasks for FER ability. Regions of interest (ROIs) related to facial emotion were obtained from a previous meta-analysis. Pearson correlation analysis was performed to understand the correlation between fALFF and FER ability. Our results indicated that the patients performed worse than the HCs in the accuracy performances of happy FER and fearful FER. The previous meta-analysis results showed that the brain regions related to FER included the bilateral amygdala (AMY)/hippocampus (HIP), right fusiform gyrus (FFG), and right supplementary motor area (SMA). Pearson correlation showed that the fALFF of the right FFG was associated with high-load fearful FER accuracy (r = -0.43, p = 0.022). Multiple regression analysis showed that the fALFF of the right FFG was an independent contributor to fearful FER accuracy. Our study indicates that FER ability is correlated with resting-state intrinsic activity in brain regions related to facial emotion, which may provide a reference for the study of FER in schizophrenia.
From ideal to real: a qualitative study of the implementation of in situ Interprofessional Simulation-Based Education
Authors: Ju Mindy , Bochatay Naike , Robertson Kathryn , Frank James , O’Brien Bridget , Schaik Sandrijn van 
Published: October 2021

Abstract
Background: Despite the widespread adoption of interprofessional simulation-based education (IPSE) in healthcare as a means to optimize interprofessional teamwork, data suggest that IPSE may not achieve these intended goals due to a gap between the ideals and the realities of implementation. Methods: We conducted a qualitative case study that used the framework method to understand what and how core principles from guidelines for interprofessional education (IPE) and simulation-based education (SBE) were implemented in existing in situ IPSE programs. We observed simulation sessions and interviewed facilitators and directors at seven programs. Results: We found considerable variability in how IPSE programs apply and implement core principles derived from IPE and SBE guidelines with some principles applied by most programs (e.g., “active learning”, “psychological safety”, “feedback during debriefing”) and others rarely applied (e.g., “interprofessional competency-based assessment”, “repeated and distributed practice”). Through interviews we identified that buy-in, resources, lack of outcome measures, and power discrepancies influenced the extent to which principles were applied. Conclusion: To achieve IPSE’s intended goals of optimizing interprofessional teamwork, programs should transition from designing for the ideal of IPSE to realities of IPSE implementation.
TanshinoneIIA exerts protective effects via attenuate FTH1, a biomarker and therapeutic target in head and neck Squamous Cell Carcinoma
Authors: Mao# Wei , Ding# Jian , Li Yu , Huang Ruofei , Wang Baoxin 
Published: October 2021

Abstract
Background
TanshinoneIIA (TanIIA) refers to one of the major lipophilic bioactive components of S. miltiorrhiza that exerts multiple pleiotropic effects (e.g., anti-inflammatory effects, antioxidant, and anti-tumor effects). Head and neck squamous cell carcinoma (HNSCC) is recognized as the sixth most common cancer worldwide. As indicated from existing studies, ferroptosis may be responsible for its progression. However, the underlying mechanisms have not been overall clarified. This study aimed to screen a functional therapeutic target in HNSCC to specifically analyze its correlation with HNSCC and whether TanIIA can act on it to exert a therapeutic effect.
Methods
Data originating from GEO series GSE6631 and GSE13398 datasets were analyzed with the limma R package on GEO2R to find differentially expressed genes between healthy people from HNSCC patients. The DEG-related PPI network was built with the STRING database, and then the visualization was conducted by Cytoscape. FTH1 expression and mapping were examined with Human Protein Atlas, and FTH1 protein analysis and Kaplan-Meier analysis were conducted to carry out survival analyses. Immunohistochemistry (IHC) and immunofluorescence (IF) were used to localize FTH1 in HNSCCs. The protein expressions were analyzed with Western-blot method. The cell survival and invasion ability were identified by employing CCK-8 and Transwell methods.
Results
A high FTH1 expression in the HNSCCs sample could indicate poor patient outcome. TanIIA at certain concentrations could significantly attenuate FTH1 expression and reduce HNSCCs survival and invasion.
Conclusions
As revealed from this study, FTH1 could act as a promising therapy target in HNSCC and can be inhibited by TanIIA, which was demonstrated to carry out anticancer activities with less side effect.
Utilizing Bioinformatics Technology to Explore the Potential Mechanism of Danggui Buxue Decoction Against NSCLC
Authors: Yu Bin , Lv Guangyao , Li Zhiyong , Li Yanli , Xu Hui 
Published: October 2021

Abstract
Background: While lung cancer poses a serious threat to human health, non–small cell lung cancer (NSCLC) is the most common type of lung cancer. Danggui Buxue Decoction (DBD) is a classic traditional Chinese medicine (TCM) commonly prescribed for anti-tumor use in China. However, the potential mechanism of DBD against NSCLC has not yet been expounded. Hence, this study clarified its potential mechanism through bioinformatics technology.Methods: First, the active ingredients of DBD and the relevant targets were searched and screened by using the TCMSP database. Then the targets of NSCLC were obtained through searching GeneCards and OMIM database. Venny diagram was drawn to find the core targets, and an ingredient-disease-target network diagram was made by Cytoscape software, followed by GO functional enrichment analysis and KEGG pathway enrichment analysis via R software, and molecular docking via the Autodock Vina software , respectively. Finally, a survival analysis was performed to evaluate the prognosis of NSCLC patients through key genes.Results: A total of 22 active ingredients of DBD were obtained. The targets found were 182 relevant to DBD and 5,773 to NSCLC, respectively, among which 140 common targets were obtained from Venny diagram. According to the enrichment degree value, five main active ingredients and ten core targets were identified. The enrichment analysis further revealed that DBD could achieve the purpose of treating NSCLC through the AGE-RAGE signaling pathway in diabetic complications. The findings from molecular docking demonstrated that quercetin and hederagenin have the best working mechanisms with PDE3A and PTGS1, and those from the survival analysis indicated that high PDE3A gene expression has a relatively poor prognosis for NSCLC patients (p < 0.05), PDE3A may be mainly distributed in the LU65 cell line originated from Asian population. Conclusions: The present study demonstrated that DBD could treat NSCLC through the synergistic correlation between multiple ingredients, multiple targets, and multiple pathways; thus, effectively improving NSCLC prognosis.
Impact of Parylene Coating On Heating Performance of Intravenous Fluid Warmer: A Bench Study
Authors: Bayoro Danielle K. , Groepenhoff Herman , Hoolihan Daniel , Rose Edward A. , Pedro Michael J. , Waldmann Andreas 
Published: October 2021

Abstract
Background
Perioperative hypothermia is a common occurrence, particularly with the elderly and pediatric age groups. Hypothermia is associated with an increased risk of perioperative complications. One method of preventing hypothermia is warming the infused fluids given during surgery. The enFlow™ intravenous fluid warmer has recently been reintroduced with a parylene coating on its heating blocks. In this paper, we evaluated the impact of the parylene coating on the new enFlow’s fluid warming capacity.
Methods
Six coated and six uncoated enFlow cartridges were used. A solution of 10% propylene glycol and 90% distilled H2O was infused into each heating cartridge at flow rates of 2, 10, 50, 150, and 200 ml/min. The infused fluid temperature was set at 4°C, 20°C, and 37°C. Output temperature was recorded at each level. Data for analysis was derived from 18 runs at each flow rate (six cartridges at three temperatures).
Results
The parylene coated fluid warming cartridge delivered very stable output of 40°C temperatures at flow rates of 2, 10, and 50 ml/min regardless of the temperature of the infusate. At higher flow rates, the cartridges were not able to achieve the target temperature with the colder fluid. Both cartridges performed with similar efficacy across all flow rates at all temperatures.
Conclusions
At low flow rates, the parylene coated enFlow cartridges was comparable to the original uncoated cartridges. At higher flow rates, the coated and uncoated cartridges were not able to achieve the target temperature. The parylene coating on the aluminum heating blocks of the new enFlow intravenous fluid warmer does not negatively affect its performance compared to the uncoated model.
Synthesis of ferrian and ferro-saponites: Implications for the structure of (Fe,Mg)-smectites formed under reduced conditions
Authors: Sakuma Hiroshi , Morida Koki , Takahashi Yoshio , Fukushi Keisuke , Noda Natsumi , Sekine Yasuhito , Tamura Kenji 
Published: October 2021

Abstract
Clay minerals are widely distributed on the surface of Earth, Mars, and Ceres in the solar systems. Among numerous clay minerals, smectites can record the history of the environment through the exchange of interlayer cations with those in water or through redox reactions with the atmosphere. Therefore, characterization of chemical compositions and crystal structures of smectites is crucial for revealing the paleoenvironment. For instance, the crystal structure within octahedral sheets of iron-bearing smectites changes to trioctahedral sheets under reduced or dioctahedral sheets under oxidizing conditions. Orbital infrared and X-ray diffraction (XRD) analyses by Mars orbiters/rovers revealed the presence of (Fe,Mg)-smectites on the surface of Mars; however, it has been difficult to characterize the properties of these (Fe,Mg)-smectites, which are rare on the surface of Earth. In this study, we synthesized ferrian (ferric ion-rich) and ferrous (ferrous ion-rich) (Fe,Mg)-saponite and revealed the effect of valence states and iron contents on the crystal structures. These saponites were synthesized using a hydrothermal method under reduced conditions. The crystal structures and valence states of iron were analyzed by XRD, Fourier-transform infrared spectroscopy, transmission electron microscopy, Mössbauer spectroscopy, and X-ray absorption near edge measurements. The synthesized clays were trioctahedral swelling clays and were identified as saponites. The valence state of iron in these synthesized saponites is altered by oxygen and a reducing agent in water; however, the trioctahedral structures are maintained under both oxidizing and reduced conditions, following a reversible reaction. This mechanism can be interpreted by the desorption and adsorption of hydrogen in the hydroxyls of the octahedral sheets of the smectite layers. The maximum basal spacing of the (02l) lattice plane in the octahedral sheets was defined by compiling various smectite data. When the basal spacing of (02l) is larger than the maximum in dioctahedral smectites, smectite can be identified as trioctahedral smectite. The redox state of iron in the octahedral sheet cannot be determined from the basal spacing of (02l). We revealed that the iron content in the trioctahedral sheet has a linear relationship with lattice parameter b. This provides a method to estimate the iron content in saponite from XRD data. The XRD profiles of smectites found at the Yellowknife Bay on Mars can be explained only by trioctahedral smectites, and the iron content in the octahedral sheet is roughly estimated to be 0.5–1.7 in a half-unit cell. These results indicate that the presence of (Fe,Mg)-saponite implies a reduced environment during the formation and that this iron-bearing saponite has both oxidation and reduction capabilities depending on the environment.
Dynamic evaluation of regional water resources carrying capacity based on set pair analysis and partial connection number
Authors: Li Zheng , Jin Juliang , Cui Yi , Zhang Libing , Wu Chengguo , Ning Shaowei , Zhou Yuliang 
Published: October 2021

AbstractIn order to quantitatively evaluate and analyze regional water resources carrying capacity (WRCC) and to describe the micro motion between connection number components, a more applicable evaluation model was sought. Firstly, an evaluation index system and grade standards for a regional WRCC were constructed. Then, a method for determining the connection number was proposed, which considered the micro motion between the connection number components in the system structure. Finally, an evaluation model based on set pair analysis (SPA) and partial connection number (PCN) that used subtraction set pair potential (SPP) to identify vulnerability factors was built, and identification results were compared with the total partial connection number (TPCN). The model was applied to Huaibei City, Anhui Province, China. The results showed that: the WRCC grade value was between 2 and 3 which was poor; the support and regulation subsystem grade value was between 2 and 3, and the pressure subsystem grade value was between 1 and 2. SPP identified that the support force and regulation force subsystem were the vulnerable subsystems. Eight indexes, including water resources per capita, rate of ecological water consumption and density of population, were the main indicators causing the poor WRCC, which were in good agreement with the local measured data. In addition, the SPP and TPCN were compared to further verify rationality of the connection number determination method and reliability of the identification results. The model established in this paper has strong applicability and can also be used for the dynamic evaluation of other resources, including the environment and ecological carrying capacity. The results in this study provide a scientific basis for water resources management and decision-making.