bluuebunny/crossref_metadata_2025_split huggingface dataset:
Extraction of Chitosan From Fish Scale for Food Preservation and Shelf-Life Enhancer
Authors: Ooi Hon Min , Munawer Mohamad Huzair , Kiew Peck Loo 
Published: December 2021

Abstract
With the growing demand for chitosan in a variety of applications and the drawbacks associated with crustacean shell-based chitosan, there is a need to look for alternative sources of chitosan extraction and production. Chitosan was extracted from the scales of red snapper ( Lutjanus johnii ) in this study. It was discovered that the concentration of solvent (HCl and NaOH) and temperature at various stages of the extraction process influenced the yield of extracted fish scale chitosan. The characterization result revealed that the commercial crustacean-based chitosan and the extracted fish scale chitosan had similar Fourier-transform infrared spectroscopy (FTIR) spectra, indicating that the two had similar functional groups. The degree of deacetylation (DDA) of the extracted fish scale chitosan was determined to be 76.9%, with the ash value, loss on drying, and solubility being 1.28%, 3.62%, and 88.8%, respectively. The extracted and commercialised chitosan were found to be similar in all characterization results. The potential of fish scale chitosan as a food preservative and shelf-life enhancer was then investigated in this study. Strawberries coated with chitosan were stored at various temperatures, and their physical appearance and moisture loss were recorded. When used in conjunction with traditional preservation techniques such as storage in a cool environment, fish scale chitosan was found to be capable of preventing up to 50% moisture loss in strawberries.
Incidence of Oncogenic HPV and HPV-Related Dysplasia Five Years After a Negative HPV Test by Self-Sampling in Elderly Women
Authors: Hermansson Ruth , Olovsson Matts , Gustavsson Inger , Gyllensten Ulf , Lindkvist Olga , Lindberg Julia Hedlund , Lillsunde-Larsson Gabriella , Lindström Annika K 
Published: December 2021

Abstract
PURPOSE: Cervical cancer prevention for older women can be challenging since there are no specific guidelines for this group. This study aimed to determine the incidence of oncogenic HPV and HPV-related dysplasia in elderly women 5 years after being HPV negative. METHODS: Invited women participated five years earlier in a study where self-sampling for HPV testing was applied, at this time, they were all HPV negative. The women were now invited to perform self-sampling for HPV testing. Women with a positive result performed a repeat HPV test. Those with a positive repeat HPV test were examined by colposcopy, biopsy and cytology. RESULTS: Of the 804 invited women, 634 (76.9%) agreed to participate in the study and a self-sampling kit was sent to them. Of these, 99.6% (632/634) sent a sample to the HPV laboratory. The participation rate in each age group was 93.3% at age 65, 74.0% at age 70, 80.7% at age 75 and 64.6 % at age 80. Overall 18 women (2.8%, 95% CI 3.2 to 6.0) were HPV positive in the first test and 8 (1.3%, 95% CI 0.6 to 2.6) in the second test. Sampling for the second test was done on average 5.4 months after the first test. Fifty per cent (4/8) of the women with a positive repeat test had dysplasia in histology. CONCLUSION: The incidence of HPV in previously HPV-negative elderly women was low. Among women who were HPV positive in a repeat test, there was a high prevalence of low grade dysplasia.
Applying Spectral Fractal Dimension to Predict the SPAD Value of Rice Leaves Under Disease Stress
Authors: Cao YiFei , Xu Huanliang , Song Jin , Yang Yao , Hu Xiaohui , Wiyao Korohou Tchalla , Zhai Zhaoyu 
Published: December 2021

Abstract
BackgroundThe chlorophyll content is a vital indicator for reflecting the photosynthesis ability of plants and it plays a significant role in monitoring the general health of plants. Since the chlorophyll content and the soil-plant analysis development (SPAD) value are positively correlated, it is feasible to estimate the SPAD value by calculating the vegetation indices (VIs) through hyperspectral images, thereby estimating the chlorophyll content. However, current indices simply adopted few wavelengths of the hyperspectral information, which may decrease the estimation accuracy. Besides, few researches explored the applicability of VIs over plant leaves under disease stress.MethodsIn this study, the SPAD value was estimated by calculating the fractal dimension of hyperspectral curves, ranging from 420 to 950 nm. The correlation between the SPAD value and wavelengths under disease stress was analyzed. In addition, a SPAD prediction model was built upon the combination of selected indices and 4 machine learning methods, including decision tree (DT), partial least square regression (PLSR), support vector regression (SVR), and back propagation neural network (BPNN). The performance of these models was compared through the correlation of determination, root mean square error, and relative error.ResultsThe results suggested that the SPAD value of rice leaves under different disease levels were sensitive to different wavelengths, meaning that the fixed wavelength selection in current indices may achieve poor estimation results. Compared with current VIs, a stronger positive correlation was detected between the SPAD value and our proposal, reaching an average correlation coefficient of 0.8263. For the prediction model, the one built with our proposal and SVR achieved the best performance, reaching R2, RMSE, and RE at 0.8752, 3.7715, and 7.8614%, respectively.ConclusionsThis work provides an in-depth insight for accurately and robustly estimating the SPAD value of rice leaves under disease stress, and our proposal is of great significance for monitoring the chlorophyll content in large-scale fields non-destructively.
Development and Feasibility of a Group, Household Level Intervention To Improve Preconception Nutrition in Nepal
Authors: Diamond-Smith Nadia , Mitchell Ashley , Cornell Alia , Gopalakrishnan Lakshmi , Johnson Mallory , Weiser Sheri , Puri Mahesh , Dahal Minakshi 
Published: December 2021

Abstract
Background: In a setting such as Nepal with malnutrition and persistent poor maternal and infant health outcomes, developing interventions to improve the nutrition of preconception and pregnant women is essential. Aside from factors related to access and knowledge about diverse and adequate diets, inequitable gender norms and women’s low status and autonomy also feed into a lack of emphasis on women’s nutrition.Methods: In this paper we describe the three phases of the design of an intervention for newly married women, their husbands, and mothers-in-law in rural Nepal. We first discuss findings from a mixed methods formative phase, and how that fed into the design of the intervention itself, in close partnership with community members. We the describe the intervention, and findings from a mixed methods feasibility and acceptability pilot among 90 participants.Results: Our formative work highlighted not just lack of awareness about nutrition, but also how factors such as women eating last, and limited mobility, played into poor diets. Low lack of communication and household and community inequitable gender norms were also important factors. Thus we designed Sumadhur, an intervention that brought groups of households (newly married wife, husband, and mother-in-law) together weekly for 4 months to strengthen relationships and gain knowledge through interactive content. We found Sumadhur to be highly feasible and acceptable by all respondents, with most (83%) attending 80% of sessions or more, the majority (73%) of participants reported “no difficulties” in attending sessions, and 99% reporting that they would like it to continue. Pre/post surveys showed a decrease in the proportion of women of women eating last and increase in knowledge about nutrition in preconception and pregnant. Qualitative interviews suggested that respondents felt it made large impacts on their lives, in terms of strengthening relationships and trust, understanding each other, and changing behaviors.Conclusions: We show how a designing an intervention in close partnership with the target recipients and local stakeholders can lead to an intervention that is able to target complicated and culturally held practices and beliefs, positively benefit health and wellbeing, and that is very well received.Trial registration: ClinicalTrials.gov NCT04383847, registered 12/05/2020https://clinicaltrials.gov/ct2/show/results/NCT04383847?view=results
Observational Study of Re-Biopsy in EGFR-TKI-Resistant Patients with EGFR Mutation-Positive Advanced NSCLC
Authors: Koyama Kenichi , Miura Satoru , Watanabe Satoshi , Shoji Satoshi , Koshio Jun , Hayashi Yoshiki , Ishikawa Daisuke , Sato Ko , Miyabayashi Takao , Okajima Masaaki , Ota Takeshi , Tanaka Tomohiro , Matsumoto Naoya , Kuriyama Hideyuki , Abe Tetsuya , Nozaki Koichiro , Ichikawa Kosuke , Kondo Rie , Tanaka Hiroshi , Kikuchi Toshiaki 
Published: December 2021

Abstract
Identification of acquired resistant mutation has been essential in non-small cell lung cancer (NSCLC) patients with epidermal growth factor receptor (EGFR) active mutations. Re-biopsy plays a pivotal role to select the optimal treatment for patients who develop resistance to initial EGFR-tyrosine kinase inhibitors (EGFR-TKIs). This multicenter, observational study was conducted to investigate the details of re-biopsy in Japanese clinical practice.The primary endpoints were the implementation rate of re-biopsy and the concordance rate for the T790M mutation detection between histology and cytology specimens using the Cobas ® EGFR mutation test v2. 194 patients with EGFR-mutant NSCLC were enrolled and 120 patients developed acquired resistance to EGFR-TKIs. The median age was 68 years (range 20-87), and 52.5% of the patients were women. Re-biopsy was performed on 109 patients with the implementation rate of re-biopsy was 90.8%. The success rate of re-biopsy in total/histology/cytology/liquid biopsy population was 78.0%, 94.9%, 83.3% and 43.8%, respectively. The positive percent agreement and the negative percent agreement in the detection of T790M mutations between the histology and cytology specimens was both of 90.9%. Aggressive obtaining histological or cytological tissue samples at re-biopsy may contribute to improvement of the detection rate of T790M mutation. (trial registration number: UMIN000026019)
Synthesis, Characterization and Photophysicochemical Properties of Zinc(Ii) Phthalocyanine With New Benzenesulfonamide Derivative Substituents
Authors: Öncül Gülen Atiye , Öztürk Ömer Faruk , Pişkin Mehmet 
Published: December 2021

Abstract
Novel (E)-4-((5-bromo-2-hydroxy-3-methoxybenzylidene)amino)-N-(pyridin-2-yl)benzenesulfonamide 1, (E)-4-((5-bromo-2-(3,4-dicyanophenoxy)-3-methoxybenzylidene)amino)-N-(pyridin-2-yl)benzene sulfonamide 2 and 2(3),9(10),16(17),23(24)-tetra-[(E)-4-((5-bromo-3-methoxy-2-(λ1-oxidanyl)benzyl idene)amino)-N-(pyridine-2-yl)benzenesulfonamide]phthalocyaninato zinc(II) phthalocyanine 3 were synthesized. Their (1-3) structures were illuminated with spectroscopic methods such as FT-IR, 1H NMR,13C NMR, UV–vis, MALDI-TOF mass spectra and also elemental analysis. The spectroscopic, photophysical and photochemical properties of the zinc(II) phthalocyanine 3 were investigated in dimethyl sulfoxide and its effects on the above mentioned properties were reported as a result of containing new type benzenesulfonamide derivatives as substituents. In addition, its above-mentioned properties were reported by comparing different species with those of their substituted and/or unsubstituted counterparts. The zinc(II) phthalocyanine 3 has photosensitizing abilities suitable and sufficient especially for photocatalytic applications.
The Fetal Airway Parameters: Potential Diagnostic and Prognostic Markers of Intrathoracic Lesions
Authors: Song Shijing , Wang Jingjing , Wang Li , Wu Qingqing 
Published: December 2021

Abstract
Objective: Aim to study the fetal airway parameters in normal fetuses and fetuses with intrathoracic lesions. Methods: This was an observational case–control study. In the control group, 77 women were screened at 20-24 weeks gestational age, 12 were screened at 24-28 weeks gestational age, and 23 were screened at 28-34 weeks gestational age. In the case group, 41 cases were enrolled (6 cases of intrathoracic bronchopulmonary sequestration, 22 cases of congenital pulmonary airway malformations, and 13 cases of congenital diaphragmatic hernia). Fetal airway parameters (tracheal width, the narrowest lumen width, width of subglottic cavity and laryngeal vestibule) were measured. The correlations between fetal airway parameters and gestational age were analyzed. The fetal airway parameter differences between the control group and the case group were analyzed. Results: Fetal airway parameters of both groups were increased and had association with gestational age. Fetal airway parameters of the case group were smaller than the control group. The tracheal width in fetuses with congenital diaphragmatic hernia was the smallest in the cases studied. Conclusion: Fetal airway parameters are expected to provide a novel diagnostic and prognostic method for intrathoracic lesions.
Reasons for Metformin Non-Use in Type 2 Diabetes Mellitus in a Hospital: A Retrospective Observational Study
Authors: Sun Yexin , Zhou Jian-Bo , Wan Yingxin , Zhang Xueying , Li Huixia , Liu Ying , Liu Xiaoqing , Wang Nan , Ye Qing , Huo Guishen 
Published: December 2021

Abstract
Objective: To investigate and analyze the reasons for metformin non-use in a hospital. Methods: Research-related non-probability physicians and patients filled questionnaire. Results: Physicians’ main influencing factors were severe liver and kidney dysfunction; gastrointestinal adverse reactions and 11 other causes. Secondary factors.included the appearance of hypoglycemia and other adverse reactions (e.g., skin rash) . Patients’ main reasons included: worry about drug’s influence on liver and kidney function, gastrointestinal adverse reactions, hypoglycemia, and further weight loss, etc. Statistical analysis showed metformin has certain effects on the mean blood glucose and the mean glycated hemoglobin levels.Conclusion: Based on the research results, we can design more targeted medication education programs.
Analysis of Microbial Community Structure and Volatile Compounds in the Pit Mud Used for Manufacturing Taorong-type Baijiu Based on High-Throughput Sequencing
Authors: Liu Yanbo , Sun Mengxiao , Hou Pei , Wang Wenya , Shen Xiangkun , Zhang Lixin , Han Suna , Pan Chunmei 
Published: December 2021

Abstract
In this study, the pit mud used in manufacturing Taorong-type Baijiu was collected from the upper, middle, lower and bottom layers of pits in Henan Yangshao Liquor Co., LTD. Besides, high-throughput sequencing (HTS) technology was adopted to analyze the microbial community structure of the pit mud. In addition, the volatile compounds in the pit mud were subjected to preliminarily qualitative analysis through headspace-solid phase microextraction (HS-SPME) and gas chromatography-mass spectrometry (GC-MS). The results of HTS demonstrated that there were 5, 3, 5 and 5 dominant bacterial phyla (including 11, 11, 9 and 8 dominant bacterial genera) and 3, 3, 3 and 3 dominant fungal phyla (including 4, 7, 7 and 5 dominant fungal genera) in the pit mud from F-S (upper), G-Z (middle), H-X (lower) and I-D (bottom), respectively. The qualitative analysis results of volatile compounds demonstrated that a total of 78 kinds of volatile compounds were detected in the pit mud, including 46, 45, 39 and 49 kinds in the pit mud from F-S, G-Z, H-X and I-D, respectively. Ester and acid were the two main components in the pit mud. Meanwhile, the correlation between microorganisms and main volatile compounds in the pit mud was analyzed. Moreover, Lentimicrobium, Syner-01 and Blvii28_wastewater-sludge group were found for the first time in the pit mud used for manufacturing Taorong-type Baijiu. The findings of this study could provide a theoretical foundation for improving the quality of pit mud and the flavor of Taorong-type Baijiu.
Characterization of Patients With Type 2 Diabetes and Chronic Kidney Disease in a Large Integrated Health System
Authors: Pantalone Kevin , Ji Xinge , Kong Sheldon , Elliott Jay , Milinovich Alex , Misra-Hebert Anita , Farej Ryan , Singh Rakesh , Bauman Janine , Zimmerman Robert , Vachharajani Tushar , Partridge Jamie , Cameron Jennifer , Williamson Todd , Sugano David , Kattan Michael 
Published: December 2021

Abstract
Background: Chronic kidney disease (CKD) poses substantial clinical, economic, and humanistic burden in patients with type 2 diabetes (T2D). Understanding of how to optimize patient care and slow disease progression is warranted.Objective: To compare the prevalence, clinical characteristics, treatment patterns, and healthcare utilization among patients with CKD, T2D, or CKD associated with T2D.Design: Retrospective cross-sectional analysis. Patients: Adult patients with CKD, T2D, or CKD+T2D in the Cleveland Clinic Health System.Main Measures: Clinical characteristics, laboratory measures, medication utilization, cardiometabolic management and healthcare resource utilization.Key Results: In 2019, 122,276 patients were identified as CKD, 63,643 were identified as T2D, and 35,255 were identified as having CKD associated with T2D. Patients with CKD and CKD+T2D were observed to be older than those with T2D alone, 72.6, 72.2, and 63.0 years, respectively. T2D and T2D+CKD cohorts contained a higher percentage of males vs. those with CKD alone, 49.3%, 50.8%, 43.6%, respectively. ACEi/ARB therapy was observed in 27.7%, 42.4%, and 31.5% patients with CKD, T2D+CKD, and T2D, respectively. SGLT-2i therapy was only prescribed to a minority of patients with T2D (5.8%) and T2D+CKD (4.7%). Emergency department visits and hospital admissions were more prevalent in the group with CKD (26.5%, 52.9%) or CKD+T2D (28.8%, 54.9%) vs. T2D alone (18.6%, 41.8%), respectively. The majority of patients with CKD (90.4%), CKD+T2D (60.0%) or T2D (69.1%) did not have an assessment of urine protein.Conclusions: Patients with CKD+T2D or CKD have higher healthcare resource utilization than those with T2D alone. The majority of patients with CKD, CKD+T2D or T2D do not undergo assessment of urine protein and are under prescribed ACEi/ARB therapy. SGLT-2i therapy was prescribed to only a small minority of patients with T2D or CKD+T2D. An opportunity exists to improve the care of patients with CKD, both with and without T2D.
Towards Reliable Probabilistic Time-Series Projections of Global Mean Surface Temperature
Authors: Sansom Philip G. , Cummins Donald , Siegert Stefan , Stephenson David B 
Published: December 2021

Abstract
Quantifying the risk of global warming exceeding critical targets such as 2.0 ◦ C requires reliable projections of uncertainty as well as best estimates of Global Mean Surface Temperature (GMST). However, uncertainty bands on GMST projections are often calculated heuristically and have several potential shortcomings. In particular, the uncertainty bands shown in IPCC plume projections of GMST are based on the distribution of GMST anomalies from climate model runs and so are strongly determined by model characteristics with little influence from observations of the real-world. Physically motivated time-series approaches are proposed based on fitting energy balance models (EBMs) to climate model outputs and observations in order to constrain future projections. It is shown that EBMs fitted to one forcing scenario will not produce reliable projections when different forcing scenarios are applied. The errors in the EBM projections can be interpreted as arising due to a discrepancy in the effective forcing felt by the model. A simple time-series approach to correcting the projections is proposed based on learning the evolution of the forcing discrepancy so that it can be projected into the future. This approach gives reliable projections of GMST when tested in a perfect model setting. When applied to observations this leads to projected warming of 2.2 ◦ C (1.7 ◦ C to 2.9 ◦ C) in 2100 compared to pre-industrial conditions, 0.4 ◦ C lower than a comparable IPCC anomaly estimate. The probability of staying below the critical 2.0 ◦ C warming target in 2100 more than doubles to 0.28 compared to only 0.11 from a comparably IPCC estimate.
Projection Micro Stereolithography (PµSL) to 3D Print a Micro-Optofluidic Device for Two-Phase Slug Flow Detection: a Comparison with a PDMS-Based Device Manufactured by Resin Casting
Authors: Saitta Lorena , Celano Giovanni , Cicala Gianluca , Fragalà Maria Elena , Stella Giovanna , Barcellona Matteo , Tosto Claudio , Bucolo Maide 
Published: December 2021

Abstract
In this work, the use of Projection Micro Stereolithography (PmSL) to 3D print a micro-optofluidic device for two-phase slug flow detection is presented. For comparison purposes a PDMS based device obtained by casting was also manufactured. The micro-optofluidic device has a microfluidic T-junction with a micro-optical section that consists of two optical fiber insertions used for two-phase slug flow detection. The working principle in the detection is based on a different light transmission correlated to the fluid interfering with the laser beam in a micro-channel section. The 3D printed material is fully characterized in terms of its surface properties and compared to PDMS used for standard construction using a master-slave casting procedure. The two devices were tested after the setup parameters for the detection were optimized using ANOVA for the 3D printed device. The comparisons of the two devices revealed that 3D printed device can be used for two-phase slug flow detection but future research is still need to obtain a 3D printed resin allowing to outperform PDMS.
Increased Transmissibility of SARS-CoV-2 Alpha Variant (B.1.1.7) in Children: Three Large Primary School Outbreaks Revealed by Whole Genome Sequencing in the Netherlands
Authors: Gorgels Koen M.F. , Alphen Lieke B. , Veer Brian M.J.W. van der , Hackert Volker H. , Hensels Audrey Y.J. , Heijer Casper D.J. , Dingemans Jozef , Savelkoul Paul H.M. , Hoebe Christian J.P.A. 
Published: December 2021

Abstract
BackgroundVariant of concern (VOC) SARS-CoV-2 alpha variant (B.1.1.7) was the dominant strain in the Netherlands between March 2021 – June 2021. We describe three primary school outbreaks due to the alpha variant using whole genome sequencing with evidence of large-scale transmission among children, teachers and their household contacts. MethodAll outbreaks described were investigated by the South Limburg Public Health Service, the Netherlands. A case was defined as an individual with a real-time polymerase chain reaction test or antigen test positive for SARS-CoV-2. Whole genome sequencing was performed on random samples from at least one child and one teacher of each affected class.ResultsPeak attack rates in classes were 53%, 33% and 39%, respectively. Specific genotypes were identified for each school across a majority of affected classes. Attack rates were high among staff members, likely to promote staff-to-children transmission. Cases in some classes were limited to children, indicating child-to-child transmission. At 39%, the secondary attack rate (SAR) in household contacts of infected children was remarkably high, similar to SAR in household contacts of staff members (42%). SAR of household contacts of asymptomatic children was only 9%. Conclusion Our findings suggest increased transmissibility of the alpha variant in children compared to preceding non-VOC variants, consistent with a substantial rise in the incidence of cases observed in primary schools and children aged 5-12 since the alpha variant became dominant in March 2021. Lack of mandatory masking, insufficient ventilation and lack of physical distancing also probably contributed to the school outbreaks. The rise of the delta variant (B.1.617.2) since July 2021 which is estimated to be 55% more transmissible than the alpha variant, provides additional urgency to adequate infection prevention in school settings.