bluuebunny/crossref_metadata_2025_split huggingface dataset:
Engineering VIGS Vectors by Modifying Movement Proteins of the 30K Family
Authors: Villar‐Álvarez David , Navarro José A. , Pallas Vicente , Sanchez‐Navarro Jesús Ángel 
Published: December 2024

ABSTRACTVirus‐induced gene silencing (VIGS) represents a particularly relevant tool in agricultural species for studying gene functionality. This study presents a novel approach for utilizing viruses belonging to the 30K family of movement proteins (MPs) as VIGS vectors. The method described here employs smaller inserts (54 bp or less) than those commonly used (100–500 bp). The developed strategy involves modifying 30K family MPs to introduce heterologous sequences of the gene of interest into their coding sequence. This approach enabled the successful induction of gene silencing in Nicotiana tabacum and Nicotiana benthamiana. Three representative viruses of the MP 30K family, alfalfa mosaic virus (AMV), cucumber mosaic virus (CMV), and tobacco mosaic virus (TMV) were employed. The capacity to induce gene silencing of small inserts (18–54 bp) was investigated, enabling to establish a correlation between insert size and silencing efficacy. This allowed the system to be calibrated to achieve partial silencing levels. The relationship between viral encapsidation and the level of gene silencing was also investigated, revealing that a high efficiency of viral encapsidation results in a reduction in the level of gene silencing achieved. Considering these findings, it can be concluded that the approach carried out with AMV, CMV, and TMV could be applied to other members of the MP 30K family. The MP 30K family comprises 20 viral genera and over 500 viral species, which can infect all agronomically significant plant species. Consequently, the strategy presented in this work could be applied to a wide range of relevant hosts.
Perineuronal Nets in Syrian Hamsters: Anatomical Localization, Sex Differences, Diurnal Variation, and Response to Social Stress
Authors: Shaughnessy Emma K. , Horne Benjamin W. , Huhman Kim L. 
Published: December 2024

ABSTRACTPurposePerineuronal nets (PNNs) are extracellular matrix proteoglycans surrounding neurons and glia. It has been suggested that PNNs are involved in the pathophysiology of multiple CNS illnesses, including stress‐related neuropsychiatric disorders like schizophrenia, major depressive disorder, and anxiety disorders.MethodBefore examining the putative role of PNNs in stress‐related responses, we described for the first time the anatomical distribution in Syrian hamsters (Mesocricetus auratus), an excellent model organism for studying social stress and circadian rhythms.ResultsWe observed PNNs throughout the hamster cortex and hippocampus but found low to no expression in subcortical regions such as the hypothalamus, thalamus, and striatum, sites where they are observed in rats and mice. We further demonstrated that PNNs are dynamically regulated in a sex‐dependent manner in response to acute social stress, specifically in hippocampal area CA1. We did not observe a difference in PNNs between the beginning of the dark versus light phase of the light–dark cycle in hamsters, despite other laboratory rodents showing diurnal variation in PNNs. Finally, we also demonstrated that there are sex differences in PNN expression in the somatosensory cortex and the basolateral amygdala in hamsters, suggesting that sex as a biological variable should be considered in studies of PNN function.ConclusionTogether, the data from the current study suggest that a comparative approach will be necessary to fully elucidate the functional role of PNNs and, further, that Syrian hamsters are a valuable model in this endeavor.
Driving Consumer Engagement for Circular Luxury Products: Two Large Field Studies on the Role of Regulatory Mode Language
Authors: Joenpolvi Emma , Mortimer Gary , Mathmann Frank 
Published: December 2024

ABSTRACTThis research investigates the effect of language on the marketing of circular luxury products. Circular economy principles are becoming important for luxury brands as consumers demand more circular and sustainable alternatives to traditional products. However, luxury literature is divergent on whether communicating circular economy principles with luxury products leads to positive or negative consumer outcomes. This is due to the perceived incompatibility of sustainability and luxury. This research investigates the influence of language used with circular luxury products in sales descriptions of second‐hand circular luxury products (Study 1) and language effects of sustainability communication on social media (Study 2) to establish impact on consumer engagement. Our analysis of combined N = 921,939 observations demonstrates that product descriptions and social media posts communicating circular principles lead to decreased consumer engagement. This is a major problem for the marketing of circular luxury products. However, this effect can be overcome by using predominant locomotion, progress‐focused language that creates a regulatory fit with circular luxury products, increasing consumer engagement. Theoretically this study shows the importance of predominant locomotion language in the marketing of circular luxury products. Luxury marketers can utilise predominant locomotion language in their brand communication to increase consumer engagement with these products.
The Influence of Consumer's Lay Rationalism on Electronic Entrepreneur‐Related Word of Mouth
Authors: Yuan Bing 
Published: December 2024

ABSTRACTConsumers often share and discuss information about entrepreneurs' behaviors on the Internet. Research has found that this electronic entrepreneur‐related word of mouth (EEWOM) has a sizable impact on brand and consumer behavior. While some research has started to examine the consequences of EEWOM, few studies have explored how different types of entrepreneur behavior (in this case, philanthropic versus economic) drive this form of WOM. Across three experiments, this paper demonstrates that philanthropic behavior (compared to economic behavior) encourages a higher intention to share EEWOM. Additionally, consumers lower in lay rationalism exhibited higher amounts of liking toward the entrepreneur, driven by their perceptions of the entrepreneur's altruism. This research sheds light on the interplay between entrepreneurial behavior, consumers' lay rationalism, and WOM and thus offers some practical implications for marketing managers.
GC–MS and HPLC–HRMS Metabolite Profiling and Biological Activities of Blanchetia heterotricha Extracts
Authors: de Lira Victor S. A. , Araújo Floricéa M. , Santos Rodrigo N. , Souza‐Neta Lourdes C. , Zambotti‐Villela Leonardo , Colepicolo Pio , Aona Lidyanne Y. S. , Ribeiro Paulo R. 
Published: December 2024

ABSTRACTBlanchetia heterotricha is a species popularly used for its medicinal properties. However, few scientific records report the investigation of its chemical composition and biological activity. Herein, a metabolomics and multivariate statistical analysis approach was used to assess the chemical composition, antioxidant, and antimicrobial activity of B. heterotricha essential oil (BHEO) and fixed extracts (nonvolatile). Thirty‐six metabolites were identified in the essential oil by gas chromatography–mass spectrometry (GC–MS), whereas 59 metabolites were identified in the fixed extracts by high‐performance liquid chromatography coupled with high‐resolution mass spectrometry (HPLC–HRMS). The essential oil and fixed extracts showed varying degrees of antioxidant and antimicrobial activities. The antioxidant activity varied from 7.27 ± 0.95 µg mL−1 for the root methanolic extract to 513.25 ± 7.77 µg mL−1 for the seed hexane extract. Extracts obtained with ethyl acetate showed the most promising antimicrobial activity, followed by extracts obtained with methanol and hexane. Multivariate statistical analysis allowed the identification of possible contributors to the observed activities within the extracts. This is the first study to assess the antimicrobial activity in any Blanchetia species. The combination of metabolomics and multivariate statistical analysis was a powerful tool to identify bioactive compounds, highlighting the potential of BHEO and fixed extracts as sources of metabolites with antioxidant and antimicrobial properties.
Pleurotus ostreatus Copper Nanoparticles: In Vitro and In Silico Evaluation of the Antioxidant, Antibacterial, and Antidiabetic Activities
Authors: Narayana M. S. Sandeep Veda , Rajesh N. , Dastagiri C. , Mobeen S. Anjum , Khadri Habeeb , Chandrasekhar T. , Prasanna V. Anu , Riazunnisa K. 
Published: December 2024

ABSTRACTThe rapid growth of nanotechnology has opened new frontiers in biomedical applications, particularly through the use of metal nanoparticles. This study explores the green synthesis of copper nanoparticles (CuNPs) using an aqueous extract of Pleurotus ostreatus (PO‐CuNPs) and their characterization through UV–visible spectroscopy, FTIR, SEM, and EDAX. The synthesized PO‐CuNPs demonstrated exceptional antioxidant activity, evident in hydrogen peroxide scavenging and phosphomolybdenum assays. Their antibacterial efficacy was significant against Bacillus subtilis (18 ± 0.11 mm inhibition zone) and moderate against other bacterial strains. The antidiabetic potential of PO‐CuNPs was confirmed by α‐amylase (82%) and β‐glucosidase (86%) inhibition assays. Molecular docking studies revealed kaempferol (−9.0 kcal) and quercetin (−9.2 kcal) as potent α‐amylase inhibitors, while myricetin (−8.4 kcal) was most effective against β‐glucosidase due to its favorable interactions. Despite high scores, rutin was excluded due to poor drug‐likeliness, highlighting kaempferol and myricetin as promising antidiabetic agents. This research highlights the promising biomedical applications of P. ostreatus–based CuNPs, particularly in managing oxidative stress, microbial infections, and diabetes, showcasing their potential as eco‐friendly therapeutic agents.
Synthesis, Antifungal Activity, and Action Mechanism of Anethole‐Derived Amide‐Urea Compounds
Authors: Liu Chuwen , Duan Wengui , Ma Xianli , Lin Guishan , Li Baoyu , Deng Huan 
Published: December 2024

ABSTRACTIn order to develop new antifungal molecules and explore further applications of natural products, 25 novel amide‐urea compounds were synthesized from anethole in this work by a few simple reactions, and structural confirmation was conducted using 1H‐nuclear magnetic resonance (1H‐NMR), 13C‐NMR, high‐resolution mass spectrometry, and Fourier transform infrared spectroscopy. Preliminary bioactivity tests were performed against eight plant pathogens. The results demonstrated that all compounds exhibited antifungal activity against the tested fungi, and 5p exhibited the most potent antifungal activity. To explore the action mechanism of the antifungal compounds, the inhibitory activity of 5p against succinate dehydrogenase (SDH) was evaluated and found to be comparable to that of boscalid. Furthermore, the binding mode of the compound to SDH was simulated by molecular docking, and similarities between 5p and boscalid's binding with SDH were identified. The results indicate that further investigation of compound 5p may prove beneficial in determining its potential as a fungicide.
Antioxidative Indole Diketopiperazine Alkaloids from Endophytic Fungi Aspergillus sp. JXC‐5
Authors: He Gui‐Ling , Shu Yan , Huang Geng , Ma Yu‐Chen , Yu Qun , Zhang Wei , Huang Lei , Yang Yin‐He , Xiao Huai , Wang Jia‐Peng 
Published: December 2024

ABSTRACTThree previously undescribed indole diketopiperazine alkaloids and seventeen known compounds were characterized by Aspergillus sp. JXC‐5 by solid fermentation. Their structures were elucidated by spectroscopic methods and high‐resolution electrospray ionization mass spectrometry, and the absolute configurations were further confirmed by electronic circular dichroism (ECD), induced CD spectra, and ML_J_DP4 methods. In the in vitro bioassays, compound 5 exhibits potent 2,2‐diphenyl‐1‐picrylhydrazyl and 2,2'‐azino‐bis(3‐ethylbenzothiazoline‐6‐sulfonic acid) (ABTS)·+ free radicals radical scavenging activities with half‐maximal inhibitory concentration values of 19.54 ± 0.39 and 26.39 ± 0.09 µM, respectively. Compounds 10–12, 14, and 15 exhibited potent ABTS·+ free radicals radical scavenging activity.
Calcium level and autophagy defect in GNE mutants of rare neuromuscular disorder
Authors: Sharma Shweta , Mashangva Fluencephila , Oswalia Jyoti , Singh Shagun , Alag Rohan , Arya Ranjana 
Published: December 2024

AbstractRare genetic disorders are low in prevalence and hence there is little or no attention paid to them in the mainstream medical industry. One of the ultra‐rare neuromuscular disorders, GNE myopathy is caused due to biallelic mutations in the bifunctional enzyme, GNE (UDP N‐acetylglucosamine‐2‐epimerase/N‐acetylmannosamine kinase). It catalyses the rate‐limiting step in sialic acid biosynthesis. There are no effective treatments for GNE myopathy as the pathomechanism is poorly understood. Pathologically, the disease is characterized by the formation of rimmed vacuoles that contain aggregates of β‐amyloid, tau, presenilin etc proteins in muscle biopsy samples. Accumulation of aggregated proteins in the cells may occur due to the failure of the regulated autophagy phenomenon. In the present study, we aim to understand the effect of GNE mutations on autophagy. The cytosolic calcium levels in GNE mutant cells were found to be altered in a GNE mutation‐specific manner. The chaperone levels, such as HSP70 and PDI, as well as autophagic markers (LC3II/I ratios) were altered in the GNE mutant cells. Treatment with BAPTA‐AM, calcium chelator, significantly restored cytosolic calcium levels in some GNE mutant cells as well as autophagic marker levels and autophagic punctae formation. The effect on the calcium signalling cascade involving CaMKKβ/AMPK/mTOR was studied in the GNE mutant cells. Our study provides insights into the role of calcium in autophagic vacuole formation in the cells with GNE mutations that will have significance towards understanding the pathomechanism of GNE Myopathy and drug target identification for the rare disease.
Wnt/β‐catenin pathway as a link between therapy resistance‐driven epithelial‐mesenchymal transition and stemness in colorectal cancer
Authors: Rocha Murilo Ramos , Castillo‐Medina Yuri Kelly , de Lima Coelho Bárbara Martins , Rios Luidy Lucas Lopes , Morgado‐Diaz Jose Andres 
Published: December 2024

AbstractThe high plasticity of cells undergoing epithelial‐mesenchymal transition (EMT) promotes increased tumor heterogeneity, and its interaction with tumor‐associated stromal cells appears to contribute to developing a stemness phenotype. Cells with these characteristics exhibit increased resistance to chemotherapy and radiotherapy, leading to disease relapse and metastasis. Here, we discuss the activation of the Wnt/β‐catenin pathway in promoting EMT and stemness within the context of cellular resistance to these therapies. We discuss whether EMT and cancer stem cells (CSCs) function in conjunction, independently, or if a link is connecting their development. We further propose that this pathway is necessary to establish a connection between these two phenotypes. And suggest that it could hinder the rise of CSCs from treatment‐induced EMT cells when inhibited. Understanding this cellular phenomenon might allow the development of new targeted therapies to improve clinical responses, particularly in colorectal cancer.
Advances in Zero‐Shot Prediction‐Guided Enzyme Engineering Using Machine Learning
Authors: Liu Chang , Wu Junxian , Chen Yongbo , Liu Yiheng , Zheng Yingjia , Liu Luo , Zhao Jing 
Published: December 2024

AbstractThe advent of machine learning (ML) has significantly advanced enzyme engineering, particularly through zero‐shot (ZS) predictors that forecast the effects of amino acid mutations on enzyme properties without requiring additional labeled data for the target enzyme. This review comprehensively summarizes ZS predictors developed over the past decade, categorizing them into predictors for enzyme kinetic parameters, stability, solubility/aggregation, and fitness. It details the algorithms used, encompassing traditional ML approaches and deep learning models, emphasizing their predictive performance. Practical applications of ZS predictors in engineering specific enzymes are discussed. Despite notable advancements, challenges persist, including limited training data for ZS predictors and the necessity to incorporate environmental factors (e.g., pH, temperature) and enzyme dynamics into these models. Future directions are proposed to advance ZS prediction‐guided enzyme engineering, thereby enhancing the practical utility of these predictors.
Forced Vasohibin‐1 Expression Increases Paclitaxel Sensitivity of Ovarian Cancer by Inhibiting Microtubule Activity
Authors: Koyanagi Takahiro , Saga Yasushi , Takahashi Yoshifumi , Tamura Kohei , Takahashi Suzuyo , Taneichi Akiyo , Takei Yuji , Mizukami Hiroaki , Fujiwara Hiroyuki 
Published: December 2024

ABSTRACTBackgroundVasohibin‐1 (VASH1), an angiogenic inhibitor, exhibits tubulin carboxypeptidase activity, which is involved in microtubule functions. Paclitaxel, the core chemotherapeutic agent for ovarian cancer chemotherapy, has a point of action on microtubules and may interact with VASH1.AimsTo examine the influence of VASH1 on intracellular tubulin detyrosination status, cyclin B1 expression, and paclitaxel chemosensitivity using VASH1‐overexpressing ovarian cancer cell lines.Methods and ResultsGene‐transfected human ovarian cancer cell lines were subjected to western blot analysis. Western blot analysis of VASH1‐overexpressing ovarian cancer cells revealed upregulated expression of detyrosinated tubulin and cyclin B1 compared with control cells. By WST‐1 assay, paclitaxel chemosensitivity of VASH1‐overexpressing ovarian cancer cells was markedly enhanced compared with that of control cells, whereas there was no significant difference in chemosensitivity to cisplatin. The forced expression of VASH1 enhanced tubulin carboxypeptidase activity and increased cyclin B1 expression, resulting in augmented paclitaxel chemosensitivity in ovarian cancer cells.ConclusionOvarian cancer treatment strategies targeting VASH1 can potentiate the effects of conventional chemotherapy by inhibiting angiogenesis and regulating microtubule activity.
Synthesis of Renewable Furan‐Based Benzoxazines and Polybenzoxazines: Recent Advances
Authors: Karlinskii Bogdan Ya. 
Published: December 2024

AbstractThe burgeoning field of materials science is currently witnessing a paradigm shift toward the utilization of renewable plant biomass as a viable chemical source for the production of sustainable materials. This trend is substantiated by a significant corpus of recent experimental and theoretical research focused on the synthesis and property analysis of such polymers. Within this context, polybenzoxazines stand out as a pioneering class of thermosetting polymers, distinguished by their exceptional thermal and mechanical characteristics, coupled with the feasibility of synthesizing their precursor monomers from eco‐friendly, renewable resources, including plant phenols and furfurylamine. Opting for furfurylamine over traditional aniline and its petroleum‐derived counterparts in the synthesis of benzoxazines not only increases the eco‐compatibility of the resultant materials but also imparts a significant alteration to their properties. This short review, spanning the last three years, delves into the principal advancements achieved in the realm of novel furan‐bearing benzoxazines and polybenzoxazines from 2021 to present, accentuating both the triumphs and challenges encountered in this burgeoning domain.
Environmentally Sustainable Preparation of Oleuropein and Its Dialdehydic Derivatives in a Simple Pharmaceutical Formulation
Authors: Nardi Monica , Bonacci Sonia , Frisina Marialaura , Oliverio Manuela , Scarpelli Rosa , Procopio Antonio 
Published: December 2024

AbstractIn this paper, Krapcho's one‐step decarbomethoxylation of oleuropein in DES is reported. Oleuropein used as reagent was extracted with water from olive leaves, widely available and inexpensive waste from the olive oil production chain. The reaction has been carried out in a series of ChCl‐based DES of increasing acidity, with or without the addition of an amount of water, under microwave and conventional heating. The antioxidant power of the best reaction mixture, both in terms of biocompatibility of the medium and conversion of the starting material, was measured and compared with a natural phenolic mixture coming from EVOO. The reported results indicate that the formulation deriving by the Krapcho's one‐step decarbomethoxylation of oleuropein in ChCl:Citric acid (1 : 1) DES can provide a ready‐to‐use “phenolic complex“ with oxygen scavenging power similar to a mixture of phenols extracted from an EVOO that meets the requirements of EFSA health claim.
“FBT Is for the Rich”: A Qualitative Study Examining Clinicians' Experiences and Perceptions of Treatment Access and Engagement for Diverse Families in Family‐Based Treatment
Authors: Sauerwein Jessica , Austin Amelia , Singh Manya , Pedram Pardis , Allan Erica , Bruett Lindsey , Eckhardt Sarah , Forsberg Sarah , Keery Helene , Kimber Melissa , Pradel Martin , Accurso Erin C. , Le Grange Daniel , Dimitropoulos Gina 
Published: December 2024

ABSTRACTObjectiveFamily‐based treatment (FBT) is the leading evidence‐based treatment for adolescent eating disorders, but research exploring access and engagement in FBT is sparse. This paper focuses on findings from a broader study, specifically addressing the social determinants of health (SDH) impeding access and engagement in FBT for diverse families (i.e., families belonging to identity groups subject to systemic barriers and prejudices).MethodsForty‐one FBT clinicians were recruited globally using purposive and snowball sampling. Clinicians participated in individual interviews or focus groups, discussing their experiences engaging diverse families in FBT. Qualitative data were transcribed verbatim and analyzed using reflexive thematic analysis.ResultsThe findings underscore the pervasive impact of SDH on equitable access to FBT, specifically, the critical need for tailored approaches by clinicians to enhance access and engagement in FBT for diverse families experiencing practical, resource, and systemic barriers. Recommendations include broader dissemination of FBT knowledge, telehealth options to mitigate geographical barriers, community resource collaborations, and sensitivity to cultural and systemic factors impacting treatment engagement.DiscussionResults of this study may inform future FBT planning (tailoring treatment approaches to address barriers), clinician training, clinical decision‐making tools, and opportunities for supporting under‐resourced families within the model, leading to more equitable FBT treatment access and engagement for diverse families.