bluuebunny/crossref_metadata_2025_split huggingface dataset:
HEARTS, minds and souls—it is time for geriatricians to bring more to continence management
Authors: Schlögl Mathias , Gordon Adam 
Published: May 2021

Abstract
Urinary incontinence (UI), the involuntary loss of urine, is a common health condition that may decrease the quality of life and which increases in incidence and prevalence with age. Recent epidemiologic data suggest an overall prevalence of 38% in women older than 60 years, increasing to 77% in older women living in nursing homes. Despite this high prevalence, incontinence remains underdiagnosed and undertreated in this age group. In a representative population of 7,000 participants drawn from the Irish Longitudinal Study of Ageing, 750 had UI of whom 285 (38%) had not sought the help of a health care professional. The reasons that older people do not seek help for incontinence are complex and multiplex. Stigma surrounding diagnosis, a sense of futility coupled to a notion that incontinence is a part of normal ageing and the fact that incontinence simply gets ‘lost’ in the midst of multimorbidity and frailty have all been shown to play a role. Active case finding has therefore been highlighted as a cornerstone of effective care in serial international guidelines.
Seasonal changes in temperate woody plant phloem anatomy and physiology: implications for long-distance transport
Authors: Ray Dustin M , Savage Jessica A 
Published: May 2021

Abstract
Seasonal changes in climate are accompanied by shifts in carbon allocation and phenological changes in woody angiosperms, the timing of which can have broad implications for species distributions, interactions and ecosystem processes. During critical transitions from autumn to winter and winter to spring, physiological and anatomical changes within the phloem could impose a physical limit on the ability of woody angiosperms to transport carbon and signals. There is a paucity of the literature that addresses tree (floral or foliar) phenology, seasonal phloem anatomy and seasonal phloem physiology together, so our knowledge of how carbon transport could fluctuate seasonally, especially in temperate climates is limited. We review phloem phenology focussing on how sieve element anatomy and phloem sap flow could affect carbon availability throughout the year with a focus on winter. To investigate whether flow is possible in the winter, we construct a simple model of phloem sap flow and investigate how changes to the sap concentration, pressure gradient and sieve plate pores could influence flow during the winter. Our model suggests that phloem transport in some species could occur year-round, even in winter, but current methods for measuring all the parameters surrounding phloem sap flow make it difficult to test this hypothesis. We highlight outstanding questions that remain about phloem functionality in the winter and emphasize the need for new methods to address gaps in our knowledge about phloem function.
A meta-analysis of the combined effects of elevated carbon dioxide and chronic warming on plant %N, protein content and N-uptake rate
Authors: Jayawardena Dileepa M , Heckathorn Scott A , Boldt Jennifer K 
Published: May 2021

Abstract
Elevated CO2 (eCO2) and high temperatures are known to affect plant nitrogen (N) metabolism. Though the combined effects of eCO2 and chronic warming on plant N relations have been studied in some detail, a comprehensive statistical review on this topic is lacking. This meta-analysis examined the effects of eCO2 plus warming on shoot and root %N, tissue protein concentration (root, shoot and grain) and N-uptake rate. In the analyses, the eCO2 treatment was categorized into two classes (&lt;300 or ≥300 ppm above ambient or control), the temperature treatment was categorized into three classes (&lt;1.5, 1.5–5 and &gt;5 °C above ambient or control), plant species were categorized based on growth form and functional group and CO2 treatment technique was also investigated. Elevated CO2 alone or in combination with warming reduced shoot %N (more so at ≥300 vs. &lt;300 ppm above ambient CO2), while root %N was significantly reduced only by eCO2; warming alone often increased shoot %N, but mostly did not affect root %N. Decreased shoot %N with eCO2 alone or eCO2 plus warming was greater for woody and non-woody dicots than for grasses, and for legumes than non-legumes. Though root N-uptake rate was unaffected by eCO2, eCO2 plus warming decreased N-uptake rate, while warming alone increased it. Similar to %N, protein concentration decreased with eCO2 in shoots and grain (but not roots), increased with warming in grain and decreased with eCO2 and warming in grain. In summary, any benefits of warming to plant N status and root N-uptake rate will generally be offset by negative effects of eCO2. Hence, concomitant increases in CO2 and temperature are likely to negate or decrease the nutritional quality of plant tissue consumed as food by decreasing shoot %N and shoot and/or grain protein concentration, caused, at least in part, by decreased root N-uptake rate.
A - 12 Pre-existing Anxiety and Vestibular Symptoms: The Relationship to Balance and Reaction Time in College Athletes
Authors: S Hurt , F Webbe , A LoGalbo 
Published: May 2021

Abstract

Objective
Pre-existing physical and mental health conditions can contribute to deficits in reaction time and balance among athletes. The purpose of this study was to investigate whether athletes who endorsed symptoms of anxiety and/or vestibular dysfunction would have slower reaction time and worse balance than athletes who did not.

Methods
College athletes (n = 361) at the Florida Institute of Technology who completed a pre-participation baseline evaluation including the Patient Health Questionnaire (PHQ-9), Sport Concussion Assessment Tool (SCAT-5) Baseline version, Balance Error Scoring System (BESS), General Anxiety Disorder Seven Item Scale (GAD-7), and Sway Balance App.

Results
T-tests indicated athletes who endorsed no anxiety were shown to have better balance on Sway than athletes who endorsed at least one symptom of anxiety, (t(237) = 2.73, p = 0.007, d = 0.31. Athletes who endorsed no vestibular symptoms were also shown to have better balance on Sway than those who endorsed at least one vestibular symptom, t(359) = 2.14, p = 0.03, d = 0.27. Correlational results demonstrated as symptoms of anxiety (r = −0.21, n = 361, p &lt; 0.001) or vestibular dysfunction (r = −0.15, n = 361, p = 0.006) increase, balance performance on Sway decreases. Post-hoc Tukey indicated athletes who endorsed either anxiety or vestibular symptoms had lower scores and thus worse balance on Sway than athletes who endorsed no symptoms (p = 0.009).

Conclusions
Findings suggest that pre-existing anxiety and vestibular symptoms have a negative impact on balance, supporting need for their inclusion in athletic concussion testing protocols.
A - 21 Mental Health Outcomes of Elite Athletes Following Sport-Related Concussion: A Prospective Study
Authors: M Mrazik 
Published: May 2021

Abstract

Objective
The purpose of this research was to elucidate the short and long-term mental health outcomes following sport-related concussion (SRC) in a population of elite athletes.

Methods
Specifically, symptoms consistent with anxiety, depression, and psychological distress were investigated in a population of 196 male professional and collegiate football players ages 18 to 34 years old. All athletes completed self-report measures (PHQ-9, GAD-7, and BSI-18) at baseline. Athletes who sustained a SRC (n = 16) in season as well as musculoskeletal (MSK) (n = 13) and healthy control (n = 21) athletes completed the PHQ-9 and GAD-7 at three time points.

Results
Results from the study indicated that athletes with a history of one or more SRCs reported greater symptoms of depression (F(2, 193) = 10.88, p &lt; 0.001, partial η2 = 0.10), anxiety (F(2, 193) = 13.67, p &lt; 0.001, partial η2 = 0.12), and psychological distress (F(2, 193) = 18.01, p &lt; 0.001, partial η2 = 0.16) at baseline in comparison to athletes with no history of SRC. Additionally, athletes who sustained a SRC reported greater levels of depression and anxiety at 24–48 hours post injury in comparison to the MSK and healthy control groups. No differences between groups were detected at later time points suggesting that this increase in depression and anxiety symptoms was short term. Finally, analysis at the individual level showed that 50% of SRC athletes demonstrated a reliable change from baseline to 24–48 hours post injury on the depression and/or anxiety self-report in comparison to 33% of MSK injured athletes and 17% of healthy controls. In addition, 58% of athletes who sustained a SRC reported a reliable decrease in depression and/or anxiety symptoms at one month return to play, suggesting that they were experiencing fewer anxiety and depression symptoms in comparison to baseline.

Conclusions
Taken together, this research suggests that a history of SRC impacts baseline self-report of depression, anxiety, and psychological distress. Furthermore, athletes who sustain a SRC experience subtle increases in depression and anxiety symptoms in the short term that appear to be unique to concussion. However, the athletes’ self-reported depression and anxiety symptoms did not reach clinical levels and were short lived.
Jutils: a visualization toolkit for differential alternative splicing events
Authors: Yang Guangyu , Cope Leslie , He Zitong , Florea Liliana 
Published: May 2021

Abstract

Motivation
Gene alternative splicing plays an important role in development, tissue specialization and disease and differences in splicing patterns can reveal important factors for phenotypic differentiation. While multiple computational methods exist to determine splicing differences, there is a need for user-friendly visualizations that present an intuitive view of the data and work across methods.

Results
We developed a toolkit, Jutils, for visualizing differential splicing events at the intron (splice junction) level. Jutils is method-agnostic, converting individual tools’ output into a unified representation and using it to create visualizations. Jutils creates three types of visualizations, namely heatmaps of absolute and Z-score normalized splice ratios, sashimi plots and Venn diagrams of results from multiple comparisons. Jutils is lightweight, relying solely on the unified data file for visualizations.

Availability and implementation
Jutils is implemented in Python and is available from https://github.com/Splicebox/Jutils.
Cortical Responses to Vagus Nerve Stimulation Are Modulated by Brain State in Nonhuman Primates
Authors: Rembado Irene , Song Weiguo , Su David K , Levari Ariel , Shupe Larry E , Perlmutter Steve , Fetz Eberhard , Zanos Stavros 
Published: May 2021

Abstract
Vagus nerve stimulation (VNS) has been tested as therapy for several brain disorders and as a means to modulate cortical excitability and brain plasticity. Cortical effects of VNS, manifesting as vagal-evoked potentials (VEPs), are thought to arise from activation of ascending cholinergic and noradrenergic systems. However, it is unknown whether those effects are modulated by brain state at the time of stimulation. In 2 freely behaving macaque monkeys, we delivered short trains of 5 pulses to the left cervical vagus nerve at different frequencies (5-300 Hz) while recording local field potentials (LFPs) from sites in contralateral prefrontal, sensorimotor and parietal cortical areas. Brain states were inferred from spectral components of LFPs and the presence of overt movement: active awake, resting awake, REM sleep and NREM sleep. VNS elicited VEPs in all sampled cortical areas. VEPs comprised early (&lt;70 ms), intermediate (70-250 ms) and late (&gt;250 ms) components. The magnitude of the intermediate and late components was largest during NREM sleep and smallest during wakefulness, whereas that of the early component was not modulated by brain state. VEPs during NREM were larger for stimuli delivered at the depolarized phase of ongoing delta oscillations. Higher pulsing frequencies generated larger VEPs. These short VNS trains did not affect brain state transitions during wakefulness or sleep. Our findings suggest that ongoing brain state modulates the evoked effects of VNS on cortical activity. This has implications for the role of ongoing cortical activity and brain state in shaping cortical responses to peripheral stimuli, for the modulation of vagal interoceptive signaling by cortical activity, and for the dose calibration of VNS therapies.
Cumulative Incidence of Infective Endocarditis in Patients with Congenital Heart Disease: A Nationwide, Case-Control Study Over Nine Decades
Authors: Snygg-Martin Ulrika , Giang Kok Wai , Dellborg Mikael , Robertson Josefina , Mandalenakis Zacharias 
Published: May 2021

Abstract

Background
Congenital heart disease (CHD) is a lifelong predisposing condition for infective endocarditis (IE). As a consequence of advances in pediatric care, the number of adults with CHD is now exceeding the number of children. The goal of the present study was to determine the cumulative incidence of IE in patients with CHD and detect temporal changes compared with controls.

Methods
Nationwide registry-based case-control study of patients with CHD born 1930–2017 matched with 10 random controls. Infective endocarditis episodes were linked using the Swedish 10-digit personal identification number.

Results
In total, 89 541 patients with CHD and 890 470 matched controls were included. In patients with CHD, 1477 IE episodes were registered and 447 episodes in controls. Patients with CHD had 8.5% cumulative incidence of IE at age 87 years, compared with 0.7% in matched controls. Incidence rate of IE per 100 000 person-years was 65.5 (95% confidence interval [CI] 62.2–68.9) and 1.8 (95% CI: 1.7–2.0) in CHD patients and controls, respectively. By age 18 years, patients with CHD had an IE incidence similar to that of 81-year-old controls. Incidence of IE differed by age but not by birth year. Bacterial etiology was registered from 1997 in half of the IE episodes; among CHD IE cases, 43.3% were caused by streptococci and 29.8% by Staphylococcus aureus.

Conclusions
Infective endocarditis remains an important complication in patients with CHD. Incidence correlate with age and the number of IE episodes are expected to increase as the CHD population grow older.
Regulation and distinct physiological roles of manganese in bacteria
Authors: Bosma Elleke F , Rau Martin H , van Gijtenbeek Lieke A , Siedler Solvej 
Published: May 2021

ABSTRACT
Manganese (Mn2+) is an essential trace element within organisms spanning the entire tree of life. In this review, we provide an overview of Mn2+ transport and the regulation of its homeostasis in bacteria, with a focus on its functions beyond being a cofactor for enzymes. Crucial differences in Mn2+ homeostasis exist between bacterial species that can be characterized to have an iron- or manganese-centric metabolism. Highly iron-centric species require minimal Mn2+ and mostly use it as a mechanism to cope with oxidative stress. As a consequence, tight regulation of Mn2+ uptake is required, while organisms that use both Fe2+ and Mn2+ need other layers of regulation for maintaining homeostasis. We will focus in detail on manganese-centric bacterial species, in particular lactobacilli, that require little to no Fe2+ and use Mn2+ for a wider variety of functions. These organisms can accumulate extraordinarily high amounts of Mn2+ intracellularly, enabling the nonenzymatic use of Mn2+ for decomposition of reactive oxygen species while simultaneously functioning as a mechanism of competitive exclusion. We further discuss how Mn2+ accumulation can provide both beneficial and pathogenic bacteria with advantages in thriving in their niches.
Seedling Emergence of the Tree Legume Clathrotropis brunnea Amshoff, Endemic from a Colombian Tropical Rainforest
Authors: Prato Andrés I , Daibes L Felipe , Pabón Miguel A , Castaño Alvaro A , Santos-Heredia Carolina , Fior Claudimar S 
Published: May 2021

Abstract
Seedling emergence is the main propagation method of forest species. Thus, we aimed to evaluate emergence aspects of the tree legume Clathrotropis brunnea Amshoff (sapán or blackheart sapán), a Fabaceae of high economic value endemic to the Colombian rainforest. We characterized the biometry of legumes and seeds and assessed seedling emergence in three experiments: (1) effect of fruit color and substrate, (2) seed conservation in storage, and (3) presoaking and seed position. Our results showed that seeds of green-yellow fruits have greater length and weight and a water content of up to 53%. Seedling emergence did not differ between green-yellow and dark-brown fruits but did differ with substrate type; nearly 80% of seedlings emerged in the sand substrate (S1), but only 62% emerged in the mixed substrate (S3). Fresh sapán seeds are nondormant, showing a recalcitrant behavior in which seedling emergence decreased after storage in all tested conditions. Both seed-sowing positions allowed an emergence of &gt;80% with a small benefit of the hilum downward, regardless of presoaking treatments. However, these differences did not affect seedling height or biomass after emergence. Our results provide basic knowledge on production of tropical seedlings, seeking species conservation, and use in restoration projects.
Decomposition of elastic potential energy and a rational metric for aftershock generation
Authors: Matsu'ura Mitsuhiro , Terakawa Toshiko 
Published: May 2021

SUMMARY
The occurrence of earthquakes can be regarded as shear fracture releasing the elastic potential energy stored in the Earth. The potential energy density of linear elastic forces is generally represented in the quadratic form of strain tensor components with the fourth-order coefficient tensor of elastic stiffness. When the material is isotropic, since the stiffness tensor is expressible as a linear combination of two independent symmetric tensors, we can decompose the elastic potential energy density into two independent parts, namely the volumetric part and the shearing part. By definition, the partial derivatives of the elastic potential energy density with respect to volumetric and shearing deformations give the corresponding generalized forces in the sense of Lagrangian mechanics: specifically, one-third of the first invariant of stress tensor (equivalent to the mean stress) to volumetric deformation and the square root of the second invariant of deviatoric stress tensor (equivalent to $\sqrt {3/2} $ times the octahedral shear stress). With these generalized forces instead of the normal and tangential stresses on a specific fault plane, we correctly represented the original concept of Coulomb's failure criterion (shear failure occurs when shearing stress is equal to shearing strength) and defined energetics-based failure stress (EFS). The change in EFS associated with the occurrence of a main fracture (ΔEFS) gives a rational metric for aftershock generation, which can be reduced to previously proposed various metrics in special cases. For example, when the level of background deviatoric stress is much higher than the magnitude of coseismic stress changes, the expression of ΔEFS is reduced to a similar form to the well-known Coulomb failure stress change (ΔCFS). Even in the energetics-based metric, the effects of pore-fluid pressure changes are essential. We theoretically examined the mechanical effects of induced and enforced pore-fluid pressure changes and elucidated that the difference between them is reflected in the focal mechanisms of aftershocks.
Unique Regulation of Coupled NaCl Absorption by Inducible Nitric Oxide in a Spontaneous SAMP1/YitFc Mouse Model of Chronic Intestinal Inflammation
Authors: Arthur Subha , Palaniappan Balasubramanian , Afroz Sheuli , Sundaram Uma 
Published: May 2021

Abstract
In the small intestine, Na:H (NHE3) and Cl:HCO3 (DRA or PAT1) exchangers present in the brush border membrane (BBM) of absorptive villus cells are primarily responsible for the coupled absorption of NaCl, the malabsorption of which causes diarrhea, a common symptom of inflammatory bowel disease (IBD). Inducible nitric oxide (iNO), a known mediator of inflammation, is increased in the mucosa of the chronically inflamed IBD intestine. An SAMP1/YitFc (SAMP1) mouse, a spontaneous model of chronic ileitis very similar to human IBD, was used to study alterations in NaCl absorption. The SAMP1 and control AKR mice were treated with I-N(6)-(1-Iminoethyl)-lysine (L-NIL) to inhibit iNO production, and DRA/PAT1 and NHE3 activities and protein expression were studied. Though Na:H exchange activity was unaffected, Cl:HCO3 activity was significantly decreased in SAMP1 mice due to a reduction in its affinity for Cl, which was reversed by L-NIL treatment. Though DRA and PAT1 expressions were unchanged in all experimental conditions, phosphorylation studies indicated that DRA, not PAT1, is affected in SAMP1. Moreover, the altered phosphorylation levels of DRA was restored by L-NIL treatment. Inducible NO mediates the inhibition of coupled NaCl absorption by decreasing Cl:HCO3 but not Na:H exchange. Specifically, Cl:HCO3 exchanger DRA but not PAT1 is regulated at the level of its phosphorylation by iNO in the chronically inflamed intestine.
Effects of Artificial Light at Night on Avian Provisioning, Corticosterone, and Reproductive Success
Authors: Injaian Allison S , Uehling Jennifer J , Taff Conor C , Vitousek Maren N 
Published: May 2021

Synopsis
Artificial light at night (hereafter “ALAN”) affects 88% of the land area in Europe and almost half of the land area in the USA, with even rural areas exposed to lights from agricultural and industrial buildings. To date, there have been few studies that assess the impacts of ALAN on both wildlife behavior and physiology. However, ALAN may alter energy expenditure and/or stress physiology during the breeding period, potentially reducing reproductive success and resulting in conservation implications. Here, we experimentally exposed adult female and nestling tree swallows (Tachycineta bicolor) to ALAN. We then measured the effects of ALAN compared with control conditions on parental behavior (provisioning rate), nestling physiology (corticosterone levels), and reproductive success (likelihood of all eggs hatching and all nestlings fledging per nest). Our results showed that ALAN-exposed females provisioned their nestlings at lower rates than control females. Although relatively weak, our results also suggested that ALAN-exposed nestlings had reduced baseline and increased stress-induced corticosterone compared with control nestlings. ALAN-exposed nestlings also showed greater negative feedback of circulating corticosterone. We found no support for our prediction that ALAN would reduce nestling body condition. Finally, we found some support for a negative effect of ALAN on the likelihood that all eggs hatched in a given nest, but not the likelihood that all nestlings fledged. Therefore, while it is possible that the behavioral and physiological changes found here result in long-term consequences, our results also suggest that direct ALAN exposure alone may not have substantially large or negative effects on tree swallows. Exposure regimes for free-living birds, such as exposure to a combination of anthropogenic disturbances (i.e., ALAN and noise pollution) or direct and indirect effects of ALAN (i.e., effects on physiology due to direct light exposure and alterations in food availability), may produce different results than those found here.