bluuebunny/crossref_metadata_2025_split huggingface dataset:
PSXIV-24 Real-time, on-site whole genome sequencing with oxford nanopore technologies’ MinION
Authors: Ostrovski Hanna , Savegnago Rodrigo Pelicioni , Huang Wen , Gondro Cedric 
Published: October 2021

Abstract
Most quantitative geneticists are traditionally trained for data analysis in genetic evaluation and genomic prediction, but rarely have extensive knowledge of molecular genetics or experience in experimental labs. Recent products, such as those launched by Oxford Nanopore Technologies (ONT), give those quantitative geneticists a comprehensible and hands-on toolkit to explore DNA sequencing. The ‘MinION’, a small DNA sequencer, is of interest for quantitative geneticists due to both the minimal learning curve and the non-proprietary USB connectivity. This device is small enough to be portable, allowing for potential real-time, on-farm sequencing. The objective of this project is to compare the whole genome sequence (WGS) output of the MinION sequencer to that of the Illumina HiSeq 4000. Blood was collected from a 6-month-old Akaushi calf born on a Michigan State University farm. DNA was extracted from the sample using the QIAamp DNA Blood Kit from Qiagen, and library DNA ligation preparation (SQK-LSK109) from ONT was used. After base-calling with guppy software (provided by ONT), the data were preprocessed and experimental runs with the MinION were compared using quality control. Finally, the data were aligned with guppy software, and was compared to the aligned WGS obtained with Illumina HiSeq. Quality results from each MinION indicate that, despite the low amount of sequence collected in each run (~225,303 reads per run), the quality of bases sequenced was high (Q≥7). The aligned data from the Illumina sequencer provided 40x coverage of the genome, with a total of 739,339,742 reads. Although the amount of data obtained with MinION is much smaller than that of Illumina HiSeq, the high quality of MinION’s data combined with its ease of use give an opportunity of genomic sequencing for users who are either inexperienced or do not have access to large genomic sequencing devices.
PSXV-2 The relationship of quantitative and qualitative indicators of meat productivity in Kazakh white-headed bulls
Authors: Lapshina Alexandra , Makaev Shakur , Lebedev Svyatoslav , Dzhulamanov Kinispay , Gerasimov Nikolay 
Published: October 2021

Abstract
Lifetime assessment and selection of animals based on meat quality indicators is difficult. For this purpose, the analysis of the correlation between the main features of the breeding value of Kazakh white-headed bulls and quantitative and qualitative indicators of meat productivity was carried out. Bulls (n = 12) was slaughtered at the age of 15 months. The results show that the live weight with an average strength correlates with carcass weight (r=0.61; P &lt; 0.05), with rib eye area (r=0.46), and the weight of visceralfat (r=0.51). It should also be noted the positive relationship of weight growth with the dry matter content in minced meat (r=0.46), which is realized due to fat deposition in the carcass (r=0.52). The lifetime assessment of the body conformation score is on average positively correlated with the content of dry matter (r=0.54) and protein (r=0.55) in m. longissimus dorsi. There is a positive correlation of the height in the sacrum with carcass weight (r=0.44), with the content of dry matter and protein in m. longissimus dorsi (r=0.46), with the concentration of monounsaturated fatty acids in meat (r=0.43), as well as a negative relationship (r=-0.43) of the height in the sacrum with the content of non-essential amino acids in meat. The content of monounsaturated fatty acids is significantly (P &lt; 0.05) determined by the growth weight (r=0.67) and carcass weight (r=0.67). A strong negative relationship (r=-0.61...-0.71) was found between the slaughter indicators and the content of polyunsaturated fatty acids. Thus, the high growth rate negatively affects the quality of beef lipids. There is an inversely proportional relationship between the weight of visceral fat and intramuscular fat (r=-0.79; P &lt; 0.01), which indicates a divergent lipogenesis in the body. The rib eye area weakly correlates with the biological value of beef. This research was performed with financial support from the project of RAS 0526-2021-0001.
PSIII-11 The effect of feeding supplemental zeolite (clinoptilolite) of two different particle sizes on measures of nitrogen utilization and nutrient digestibility in finishing beef heifers
Authors: Myers Cheyanne A , de Haro Marti Mario , Chahine Mireille , Chibisa Gwinyai E 
Published: October 2021

Abstract
Clinoptilolite (CLN), could potentially improve nitrogen (N) utilization when fed to beef cattle as it can bind ruminal-ammonia-N (NH3-N), limiting its loss and subsequent detoxification into urea-N, which is released into blood and is excreted in urine. However, the effectiveness of CLN is influenced by physical properties such as particle size. Although decreasing the particle size has been shown to increase the binding of ammonium in-vitro, this remains to be evaluated in vivo. Therefore, the objective of this study was to determine the effects of feeding CLN of two different particle sizes (30 and 400 µm) on ruminal NH3-N and plasma-urea-N (PUN) concentrations, ruminal pH, and nutrient intake and apparent total-tract digestibility. Six ruminally-cannulated beef heifers (mean initial BW± SD, 620.8 ± 30.15) were used in a replicated 3 × 3 Latin square design with 21 d periods (sample collection from d 15 to 21). Dietary treatments were: 1) finishing ration with no supplement (CON), 2) CON +30-µm CLN (CL-30), and 3) CON + 400-µm CLN (CL-400). Clinoptilolite was top-dressed (2.5% of diet DM) during morning feeding. Intake was measured daily. Ruminal fluid was collected on d 19 for NH3-N analysis and blood was collected 3 h post-feeding on d 21 for PUN analysis. Indwelling pH loggers were used to measure ruminal pH (d 15 to 21) and grab fecal samples were collected from d 19 to 21 to determine total-tract nutrient digestibility. Statistical analysis was conducted using PROC MIXED in SAS. There was no treatment effect (P ≥ 0.13) on ruminal NH3-N and PUN concentrations, ruminal pH, and nutrient (DM, OM, NDF, ADF and CP) intake and apparent total tract digestibility. In conclusion, feeding CLN to finishing heifers had no effect on measures of N utilization, ruminal pH and nutrient intake and apparent total-tract digestibility.
PSIV-14 Evidence for functional G-coupled protein receptors 43 and 120 in subcutaneous and intramuscular adipose tissue of Angus crossbred steers
Authors: Westbrook Lindsay , Smith Stephen , Gang Gyoungok 
Published: October 2021

Abstract
We conducted experiments to demonstrate functional G-coupled protein receptor 43 (GPR43) and GPR120 in bovine intramuscular (i.m.) and subcutaneous (s.c.) adipose tissues. We hypothesized that media volatile fatty acids and long-chain fatty acids would affect cAMP concentrations differently in i.m. and s.c. adipose tissue, which would be dependent on GPR receptor populations in the adipose tissue sites. Fresh s.c. and i.m. adipose tissue from the 5th-8th longissimus thoracic rib muscle section of Angus crossbred steers (approximately 20 mo of age) was transferred immediately to 6-well culture plates containing 3 mL of KHB/Hepes/5 mM glucose. Samples were pre-incubated with 0.5 mM theophylline plus 10 μM forskolin for 30 min, after which increasing concentrations of acetate or propionate (0, 10–3, 10–2.3, and 10–3 M) in the absence or presence of 100 μM oleic acid (18:1 n-9) or 100 µM palmitic acid (16:0) were added to the incubation media. Acetate had no effect on forskolin-stimulated cAMP production in s.c. adipose tissue but decreased cAMP in i.m. adipose tissue (P &lt; 0.05); this indicates a functional GPR43 receptor in i.m. adipose tissue. The combination of 10–2 M acetate and oleic acid decrease cAMP production in s.c. adipose tissue, consistent with GPR120 receptor activity, but oleic acid and palmitic acid attenuated the depression of cAMP production caused by acetate in i.m. adipose tissue. Palmitic acid depressed cAMP production in s.c. adipose tissue, and increased cAMP production in i.m. adipose tissue (P &lt; 0.05). Propionate had no effect on cAMP production in s.c. or i.m. adipose tissue. These results provide evidence for functional GPR43 receptors in i.m. adipose tissue and GPR120 receptors in s.c. adipose tissue, both of which would suppress lipolysis. Further research may allow producers to increase marbling with exacerbating carcass fatness through pharmacological or dietary strategies.
PSIV-10 Effects of chronic molybdenum exposure in drinking water or feed on molybdenum and copper status and production performance of gestating and lactating beef cattle consuming a low-quality forage diet
Authors: Thorndyke Meghan P , Guimaraes Octavio , Medrado Matheus , Tangredi Briana V , Yi Loh Huey , Barrington Rebecca K , Casagrande Renan R , Schmitt Ken , Zervoudakis Joanis T , Tillquist Nicole M , Li Liping , Ippolito Jim A , Wagner John J , Engle Terry E 
Published: October 2021

Abstract
The objective of this experiment was to evaluate the effects of Mo source (feed or water) on performance and mineral status of cows and calves fed a forage-based diet for two years (DM basis: 6.6% CP; 0.14% S, 6.7 mg Cu/kg, 2.4 mg Mo/kg). Fifty-four cow-calf pairs were stratified by cow age, BW, and liver Cu and Mo status, and randomly assigned to one of six treatments. Treatments consisted of: 1) Negative control (NC; no supplemental Mo or Cu); 2) Positive control [PC: NC + Cu (3 mg Cu/kg diet DM from CuSO4·5H2O)]; 3) NC + 500 µg Mo/L from MoNa2O4·2H2O in drinking water (Mo 500-water); 4) NC + 1000 µg Mo/L of MoNa2O4·2H2O in drinking water (Mo 1000-water); 5) Mo 1000-water + 3 mg Cu/kg diet DM from CuSO4·5H2O (Mo 1000-water+Cu); and 6) NC + 3 mg Mo/kg diet DM from MoNa2O4·2H2O (3.0 Mo-diet). Cattle were allowed ad-libitum access to feed, water, and a protein supplement throughout the experiment. During the winter months animals were housed in three replicate pens per treatment and during the summer months animals were housed in separate pastures by treatment where cow and calf feed and water intake could be measured separately. Data were analyzed as a completely randomized block design. Throughout the experiment no signs of molybdenosis were observed. There was a treatment x time (P &lt; 0.01) interaction for cow liver Cu concentrations. Cows not receiving supplemental Cu had lower (P &lt; 0.01) liver Cu concentrations than cows receiving supplemental Cu. Cow and calf performance, calf mineral status, cow liver Mo, and plasma and milk Mo and Cu concentrations were similar across treatments. These data indicate that Mo supplemented in water or feed at concentrations used in this experiment had minimal impact on Cu status and overall animal performance.
Three Themes for the Future of Brands in a Changing Consumer Marketplace
Authors: Campbell Margaret C , Price Linda L 
Published: October 2021

Abstract
The purpose of our conceptual introduction is to theorize how brands will continue to be relevant in the future marketplace. We identify three themes that emerge in this special issue that offer intriguing directions for future exploration and managerial action. The first theme is that because of brands’ pervasiveness, consumers have developed a meta concept of “brands” that shapes how consumers think about the market, themselves, and others. While marketers consider the power of a particular brand as a valued consumer resource, this theme speaks to the power of “brands” as a category. The second theme contributes to a growing conversation highlighting consumer agency in relation to brands. Consumers manage their relationships with brands; selectively draw on media to create their own brand narratives; and can even upend the “rules” of brand management. The final theme is that brand owners must balance continuity and change, recognizing that brand contestation and cultural change are both inevitable. Brands need to actively consider the sometimes polarized, contesting and agentic voices of consumers and other actors, finding ways to positively influence firm and societal outcomes. We hope this special issue spurs continued new research to imagine future complex dynamics of consumers and brands.
“Just Enough Mystery”: Multivocal Afterlives of a Tokugawa Refugee in Japan and the United States, 1868–2018
Authors: Gedacht Anne Giblin 
Published: October 2021

Abstract
This article examines the life and afterlives of Okei, a political refugee from the Japanese Boshin Civil War (1868–69), who became the first Japanese woman interred in North American soil. Looking at a shared space of commemoration, in this case a grave site in California, this paper tracks how Okei’s memorialization on both sides of the Pacific constructed specific communal identities over a hundred and fifty years. Okei’s legacy has been consciously constructed by different actors to at once prove the resiliency of the Japanese spirit, provide an origin story for Japanese America, glorify Japanese expansionism, promote U.S.–Japanese friendship, establish regional prestige, and embody internationalism. Despite these multivocal trans-Pacific interpretations, all renderings of Okei’s story share one key similarity: the manipulation of history to empty Okei’s life of its divisive political context. A history replaced by multiple myths, the Okei narrative came to express contrasting yet parallel identities grounded in that lonely hill in California.
Pollen-specific gene SKU5-SIMILAR 13 enhances growth of pollen tubes in the transmitting tract in Arabidopsis
Authors: Zhang Ming Jun , Zhao Tian Yi , Ouyang Xiu Ke , Zhao Xin-Ying , Dai Xinren , Gao Xin-Qi 
Published: October 2021

Abstract
Pollen tube growth and penetration in female tissues are essential for the transfer of sperm to the embryo sac during plant pollination. Despite its importance during pollination, little is known about the mechanisms that mediate pollen tube growth in female tissues. In this study, we identified an Arabidopsis thaliana pollen/pollen tube-specific gene, SKU5-SIMILAR 13 (SKS13), which was critical for the growth of pollen tubes in the transmitting tract. The SKS13 protein was distributed throughout the cytoplasm and pollen tube walls at the apical region. In comparison with wild-type pollen tubes, those of the sks13 mutants burst more frequently when grown in vitro. Additionally, the growth of sks13 pollen tubes was retarded in the transmitting tract, thereby resulting in decreased male fertility. The accumulation of pectin and cellulose in the cell wall of sks13 pollen tubes was altered, and the content of jasmonic acid (JA) in sks13 pollen was reduced. The pollen tubes treated with an inhibitor of JA biosynthesis grew much more slowly and had an altered distribution of pectin, which is similar to the pollen tube phenotypes of the SKS13 mutation. Our results suggest that SKS13 is essential for pollen tube growth in the transmitting tract by mediating the biosynthesis of JA that modifies the components of pollen tube cell walls.
Progress and challenges in sorghum biotechnology, a multipurpose feedstock for the bioeconomy
Authors: Silva Tallyta N , Thomas Jason B , Dahlberg Jeff , Rhee Seung Y , Mortimer Jenny C 
Published: October 2021

Abstract
Sorghum [Sorghum bicolor (L.) Moench] is the fifth most important cereal crop globally by harvested area and production. Its drought and heat tolerance allow high yields with minimal input. It is a promising biomass crop for the production of biofuels and bioproducts. In addition, as an annual diploid with a relatively small genome compared with other C4 grasses, and excellent germplasm diversity, sorghum is an excellent research species for other C4 crops such as maize. As a result, an increasing number of researchers are looking to test the transferability of findings from other organisms such as Arabidopsis thaliana and Brachypodium distachyon to sorghum, as well as to engineer new biomass sorghum varieties. Here, we provide an overview of sorghum as a multipurpose feedstock crop which can support the growing bioeconomy, and as a monocot research model system. We review what makes sorghum such a successful crop and identify some key traits for future improvement. We assess recent progress in sorghum transformation and highlight how transformation limitations still restrict its widespread adoption. Finally, we summarize available sorghum genetic, genomic, and bioinformatics resources. This review is intended for researchers new to sorghum research, as well as those wishing to include non-food and forage applications in their research.
COVID-19: On the Disparity in Outcomes Between Military and Civilian Populations
Authors: Riley Pete , Ben-Nun Michal , Turtle James , Bacon David , Owens Akeisha N , Riley Steven 
Published: October 2021

ABSTRACT

Introduction
The CoronaVirus Disease 2019 (COVID-19) pandemic remains a formidable threat to populations around the world. The U.S. Military, in particular, represents a unique and distinguishable subset of the population, primarily due to the age and gender of active duty personnel. Current investigations have focused on health outcome forecasts for civilian populations, making them of limited value for military planning.

Materials and Methods
We have developed and applied an age-structured susceptible, exposed, infectious, recovered, or dead compartmental model for both civilian and military populations, driven by estimates of the time-dependent reproduction number, R(t), which can be both fit to available data and also forecast future cases, intensive care unit (ICU) patients, and deaths.

Results
We show that the expected health outcomes for active duty military populations are substantially different than for civilian populations of the same size. Specifically, while the number of cases is not expected to differ dramatically, severity, both in terms of ICU burdens and deaths, is substantially lower.

Conclusions
Our results confirm that the burden placed on military health centers will be substantially lower than that for equivalent-sized civilian populations. More practically, the tool we have developed to investigate this (https://q.predsci.com/covid19/) can be used by military health planners to estimate the resources needed in particular locations based on current estimates of the transmission profiles of COVID-19 within the surrounding civilian population in which the military installation is embedded. As this tool continues to be developed, it can be used to assess the likely impact of different intervention strategies, as well as vaccine policies; both for the current pandemic as well as future ones.
Association of Military Employment With Late-Life Cognitive Decline and Dementia: A Population-Based Prospective Cohort Study
Authors: Power Melinda C , Murphy Alia E , Gianattasio Kan Z , Zhang Y i , Walker Rod L , Crane Paul K , Larson Eric B , Gibbons Laura E , Kumar Raj G , Dams-O’Connor Kristen 
Published: October 2021

ABSTRACT

Introduction
As the number of U.S. veterans over age 65 has increased, interest in whether military service affects late-life health outcomes has grown. Whether military employment is associated with increased risk of cognitive decline and dementia remains unclear.

Materials and Methods
We used data from 4,370 participants of the longitudinal Adult Changes in Thought (ACT) cohort study, enrolled at age 65 or older, to examine whether military employment was associated with greater cognitive decline or higher risk of incident dementia in late life. We classified persons as having military employment if their first or second-longest occupation was with the military. Cognitive status was assessed at each biennial Adult Changes in Thought study visit using the Cognitive Abilities Screening Instrument, scored using item response theory (CASI-IRT). Participants meeting screening criteria were referred for dementia ascertainment involving clinical examination and additional cognitive testing. Primary analyses were adjusted for sociodemographic characteristics and APOE genotype. Secondary analyses additionally adjusted for indicators of early-life socioeconomic status and considered effect modification by age, gender, and prior traumatic brain injury with loss of consciousness TBI with LOC.

Results
Overall, 6% of participants had military employment; of these, 76% were males. Military employment was not significantly associated with cognitive change (difference in modeled 10-year cognitive change in CASI-IRT scores in SD units (95% confidence interval [CI]): −0.042 (−0.19, 0.11), risk of dementia (hazard ratio [HR] [95% CI]: 0.92 [0.71, 1.18]), or risk of Alzheimer’s disease dementia (HR [95% CI]: 0.93 [0.70, 1.23]). These results were robust to additional adjustment and sensitivity analyses. There was no evidence of effect modification by age, gender, or traumatic brain injury with loss of consciousness.

Conclusions
Among members of the Adult Changes in Thought cohort, military employment was not associated with increased risk of cognitive decline or dementia. Nevertheless, military veterans face the same high risks for cognitive decline and dementia as other aging adults.
Dermatofibrosarcoma Protuberans in Active Duty Military Personnel: A Case Report
Authors: Nam Soyun , Park Jae Berm , Kim Hyun-Chul 
Published: October 2021

ABSTRACT
Dermatofibrosarcoma protuberans (DFSP) is an uncommon, soft tissue sarcoma with a high rate of local recurrence, low rate of distant metastasis, and infiltrative growth. We report two cases of DFSP in young active duty military personnel. Both cases underwent primary excision at the Armed Forces Capital Hospital with positive surgical margins and underwent re-excision at Samsung Medical Center. Dermatofibrosarcoma protuberans is a rare finding, and wide local excision with or without skin flap remains the procedure of choice. Awareness of DFSP in young patients is important for proper diagnosis and treatment and to achieve local control. Complete excision of the tumor with a clear surgical margin and thorough surveillance after surgery are recommended for service members to maintain their active duty status and to prevent ineffective combat conditions.