bluuebunny/crossref_metadata_2025_split huggingface dataset:
Study of Salivary Enzymes in Oral Lesions
Authors: Chavan Reshma P , Ingole Shivraj M , Dalvi Shubhangi M 
Published: March 2021

ABSTRACTAim and objectiveSaliva contains different enzymes, which reflect metabolic activities of the body. Certain enzymes, such as, aspartate aminotransferase (AST) and alanine aminotransferase (ALT) enzymes are products of cell injury and cell death. Some benign oral conditions having malignant potential and therefore early changes in salivary enzyme level may be helpful for the early detection of malignancy. An attempt is made to compare salivary enzyme levels (AST and ALT) in benign oral lesions in tobacco addicts, malignant oral lesions in tobacco addicts, and healthy non-tobacco addict patients with age- and sex-matched control group.Material and methodsA retrospective study of 150 patients with benign and malignant oral lesions with tobacco addiction was done. Control group includes tobacco non-addicts. The oral lesion was noted. Biopsy of oral lesion was sent to histopathology for diagnosis. The saliva of all patients was collected for salivary enzymes (AST and ALT) examination. Levels of salivary enzymes (AST and ALT) in benign and malignant oral lesions of patients with tobacco addiction and tobacco non-addict control group without any oral lesions with age- and sex-matched study group were included in the study. The results were analyzed statistically by Student's t-test.ResultsSalivary enzymes (AST and ALT) were not significantly increased in benign and malignant oral lesions in tobacco addict patients.ConclusionSalivary enzymes were increased in all benign and malignant oral lesions in tobacco consumers. There was no significant increase in salivary enzymes in all benign and malignant oral lesions in tobacco consumers.Clinical significanceResearch is going on salivary biomarkers, but these salivary biomarkers lack sensitivity and specificity and cannot be applied.How to cite this articleChavan RP, Ingole SM, Dalvi SM. Study of Salivary Enzymes in Oral Lesions. Int J Otorhinolaryngol Clin 2020;12(1):1–3.
Breast implant-associated anaplastic large cell lymphoma (BIA-ALCL) in Poland: analysis of patient series and practical guidelines for breast surgeons
Authors: Pluta Piotr , Giza Agnieszka , Kolenda Małgorzata , Fendler Wojciech , Braun Marcin , Chudobiński Cezary , Chałubińska-Fendler Justyna , Araszkiewicz Michał , Loga Karolina , Lembas Lubomir , Witkowska Magdalena , Pluta Agnieszka , Kolasiński Jerzy , Basta Pawel , Kołacińska-Voytkuv Agnieszka , Cieśla Sławomir , Jesionek-Kupnicka Dorota , Jankau Jerzy , Kuczyński Maciej , Kalinka Ewa , Zadrożny Marek , Murawa Dawid 
Published: March 2021

IntroductionAlthough breast implant-associated anaplastic large cell lymphoma (BIA-ALCL) is infrequent, with less than 1000 noted cases worldwide, patients consenting for breast implant surgery should be aware of its risk. We describe the first Polish multicenter case-series data on BIA-ALCL patients and present diagnostic and treatment recommendation for breast surgeons.Material and methodsIn cooperation with the Polish Society of Surgical Oncology and Polish Lymphoma Research Group, we collected BIA-ALCL cases in Poland.ResultsWe retrospectively reviewed clinical data of seven BIA-ALCL patients, diagnosed between July 2013 and November 2019. The median time from implant placement to the first BIA-ALCL symptoms was 65 months (range: 33–96 months). All the patients were exposed to textured implants at presentation. Capsulectomy with implant removal was performed in all the patients with immediate reimplantation in 2 cases. In a median follow-up of 19 months (range 5-81 months), there was no recurrence and all the patients stayed alive. Between 2013 and 2019, the incidence of BIA-ALCL in Polish female population age 30 and above ranged from 0 to 0.021/100 000/year.ConclusionsBIA-ALCL is scarce in the Polish population. In a short-term follow-up, patients’ prognosis remains excellent. Due to the withdrawal of roughly textured implants from the market and the exclusion of likely the most potent etiologic factor, it might be expected that the incidence of BIA-ALCL will become even rarer.
Oleuropein modulates over-proliferation
of keratinocytes in mice with imiquimodmediated
psoriasis and inhibits differentiation of TH17 cells
through the JAK3/STAT3 axis
Authors: Shi Quan , He Qi , Chen Weiming , Long Jianwen , Zhang Bo 
Published: March 2021

IntroductionOleuropein (OLP) is polyphenol obtained from olive oil; it is proved in Chinese traditional medicine for its use in disorders including autoimmune and inflammatory disorders. Psoriasis (PSR) is an autoimmune and inflammatory disorder triggered by T-helper-17 (Th17) cells.Material and methodsWe developed an imiquimod (IMQ)-mediated PSR model in mice to study the anti-inflammatory role of OLP in psoriasis. The mice were given 50 mg/kg and 100 mg/kg dose of OLP. Histology was done to assess the inflammation of lesions. Western blot analysis was done for JAK3/STAT3 in isolated T cells, expression of RORgt was done by RT-PCR. The In silico molecular docking studies were done for interaction of OLP with target protein STAT3 and JAK3.ResultsTreatment of OLP attenuated proliferation in IMQ-mediated keratinocytes, improved infiltration of CD3+ cells in the skin lesions and in CD4+ and CD8+ T cells and also ameliorated the levels of cytokines. In in vitro studies in isolated T cells, OLP blocked the differentiation of Th17 cells and also the levels of IL-17 and the JAK3/STAT3 pathway. The in silico docking showed that OLP had potential binding affinity with JAK3 and STAT3 which was parallel to in vivo and in vitro findings.ConclusionsOLP ameliorates psoriasis skin lesions by blocking Th17-mediated inflammation. OLP may be an interesting molecule for treating autoimmunity in psoriasis.
Important role of long non-coding RNA UBE2R2-AS1 in diabetes-induced renal injury
Authors: Chen Chuanqi , Yan Yanqiong , Luo Qianjun , Li Sufen 
Published: March 2021

IntroductionThe present study examined the effects and mechanisms underlying long non-coding RNA (lncRNA) UBE2R2-AS1 in diabetes-induced renal injury.Material and methodsHigh glucose was used to imitate diabetes-induced renal injury in a cell model. Cell apoptosis rate was measured using flow cytometry, tumour necrosis factor-a (TNF-a) and interleukin-6 (IL-6) concentrations were evaluated using ELISA, and relative protein expression and amount were measured using western blot (WB) analysis and immunofluorescence, respectively. Correlations between lncRNA UBE2R2-AS1, miRNA-877-3p and TLR4 were analysed using the luciferase reporter assay.ResultsCell apoptosis rate and TNF-a and IL-6 concentrations were significantly higher (p < 0.001) in the high glucose (model) group compared with those of the Control group (CG) group. Following shRNA knockdown of lncRNA UBE2R2-AS1, the cell apoptosis rate and TNF-a and IL-6 concentrations were significantly lower (p < 0.001, respectively) compared with those of the model group. However, under high-glucose conditions, shRNA knockdown of UBE2R2-AS1 and miRNA-877-3p significantly increased (p < 0.001) the cell apoptosis rate as well as TNF-a and IL-6 concentrations compared with the shRNA UBE2R2-AS1 knockdown group. WB and immunofluorescence analysis showed that toll-like receptor 4 (TLR4)/myeloid differentiation factor 88 (MyD88)/nuclear factor kB (p65) (NF-kB(p65)) pathway proteins were significantly stimulated in the model group compared with those in the CG, whereas shRNA transfection with miRNA-877-3p stimulation suppressed the TLR4/MyD88/NF-kB(p65) pathway.ConclusionsKnockdown of lncRNA UBE2R2-AS1 improves diabetes-induced renal injury via regulation of the miRNA-877-3p/TLR4 axis in vitro.
Vitamin D ameliorated endothelial cell damage induced by diabetes via regulation of lncRNA MEG3 in human umbilical vein endothelial cells
Authors: Wei Hua , Zhang Qiongfang , Li Jun , Yang Jing , Huang Bin , Huang Chun-chun , Wang Ying-chuan , Hu Jing-qian , Wei Xi 
Published: March 2021

IntroductionThe purpose of this study was to investigate long non-coding RNA (lncRNA) maternally expressed gene 3 (MEG3) effects in vitamin D (Vit. D) treatment in endothelial cell damage induced by diabetes.Material and methodsWe used human umbilical vein endothelial cells (HUVECs) as a research objective in our study and used high glucose in a diabetic cell model. We evaluated cell apoptosis by flow cytometry, inflammatory factors (IL-6, IL-1β and TNF-α) concentrations by ELISA assay, relative gene and protein expression by RT-qPCR and WB assay, and NF-κB(p65) nuclear volume by cellular immunofluorescence.ResultsCompared with the NC (normal control) group, the cell apoptosis rate was significantly increased, inflammatory factor (IL-6, IL-1β and TNF-α) concentrations were significantly up-regulated, lncRNA MEG3 gene expression was significantly depressed, Toll-like receptor 4 (TLR4), myeloid differentiation factor 88 (MyD88) and nuclear factor B p65 (NF-κB(p65)) gene and protein expression levels were significantly increased and NF-κB(p65) nuclear volume was significantly up-regulated (p < 0.001, respectively). With Vit. D supplementation, compared with the Model group, Vit. D improved endothelial cell damage induced by diabetes, while lncRNA MEG3 was significantly increased and the TLR4/MyD88/NF-κB(p65) pathway was significantly depressed dose-dependently (all p < 0.05). With sh-MEG3 transfection, the Vit. D treatment effects were significantly reduced.ConclusionsVit. D improved endothelial cell damage induced by diabetes via lncRNA MEG3 up-regulation in vitro study.
The mechanism of treatment of multiple myeloma
with metformin by way of metabolism
Authors: Song Yanyan , Chen Song , Xiang Wei , Xiao Muran , Xiao Hui 
Published: March 2021

IntroductionMultiple myeloma (MM) is a malignant hematologic tumor. Although many new drugs are currently found to significantly improve the median survival, MM is still not curable due partly to drug resistance recurrence. Epidemiological studies have shown that patients with type 2 diabetes have a high risk of malignancy, and patients’ treatment with metformin could reduce the risk of cancer as well as associated mortality.Material and methodsWe used chemotherapeutics – melphalan combined with metformin or the single drug – to treat RPMI8226 cells and used a series of tests to detect the drug sensitivity, apoptotic rate, DNA damage and the concentration of ATP. SPSS 17.0 was used to analyze the data.ResultsThe inhibitory effect of melphalan on RPMI8226 cells was significantly increased after metformin was added (p < 0.05), and the inhibitory effect was enhanced with the increasing concentration of melphalan. The comet assay showed that metformin increased melphalan-induced DNA damage and increased the apoptotic rate from 12.7 ±2.8% to 18.8 ±1.5% (p < 0.05). In the ATP concentration test, the concentration of ATP in the tumor cells was significantly decreased from 0.42 ±0.01 µmol/l to 0.08 ±0.02 µmol/l (p < 0.05).ConclusionsMetformin can promote DNA damage induced by melphalan and decrease the concentration of ATP in the process of repairing DNA damage to hinder the anti-apoptotic process of tumor cells, which showed the pesticide effect of the enhanced sensitivity of multiple myeloma cells to melphalan.
Gastrointestinal haemorrhage in extracorporeal membrane oxygenation: insights from the national inpatient sample
Authors: Solanki Shantanu , Haq Khwaja , Jolly George , Chakinala Raja Chandra , Khan Muhammad Ali , Patel Neil , Bhurwal Abhishek , Haq Khwaja , Nabors Christopher , Ganatra Sarju , Aronow Wilbert 
Published: March 2021

IntroductionExtracorporeal membrane oxygenation (ECMO) is associated with gastrointestinal haemorrhage (GIH), which may result from coagulo­pathy, systemic inflammation, reduced gastric perfusion, and arteriovenous malformation from non-pulsatile blood flow. Data are limited regarding the burden of this complication in the United States.Material and methodsWe analysed the National Inpatient Sample (NIS) database for the years 2007 to 2011 to identify hospitalisations in which an ECMO procedure was performed. Hospitalizations complicated by GIH in this cohort were then identified by relevant codes.ResultsBetween 2007 and 2011, ECMO hospitalisations increased from 1869 to 3799 (p < 0.01). The proportion of hospitalisations complicated by GIH increased from 2.12% in 2007 to 7.46% in 2011 (p < 0.01). Gastrointestinal haemorrhage was more common in men (56.7%) and in Caucasians (57.4%). Common comorbidities in this population were renal failure (71%), anaemia (55%), and hypertension (26%). All-cause inpatient mortality showed a numerical but nonsignificant increase from 56.7% to 61.9% (p = 0.49). The average cost of care per hospitalisation with GIH associated with ECMO use increased from $132,420 in 2007 to $215,673 in 2011 (p < 0.01).ConclusionsGastrointestinal haemorrhage during ECMO hospitalisations occurred in small but significantly increasing proportions. The inpatient mortality rate and costs associated with GIH were substantial and increased significantly during the study period.
Correlation analysis of adjuvant chemotherapy duration and postoperative death in Chinese patients with colorectal cancer: a retrospective cohort study
Authors: Chen Weiwei , Wang Gang , Dong Hongmin , Wang Wenling 
Published: March 2021

IntroductionIt is not known whether adjuvant chemotherapy duration is non-linearly associated with postoperative mortality risk. This study was designed to examine this relationship in Chinese patients with colorectal cancer.Material and methodsThis cohort study was conducted in 1171 participants with stage III and high-risk stage II colorectal cancer, who were treated in China between July 1, 2011, and September 1, 2018. The target independent variable was adjuvant chemotherapy duration, and the dependent variable was postoperative death.ResultsAfter adjusting for confounding factors, an increase in adjuvant chemotherapy duration was negatively correlated with the risk of postoperative death (hazard ratio (HR) = 0.95, 95% confidence interval (CI): 0.900.99). However, a non-linear relationship between therapy duration and postoperative death was observed in the group with bolus and infused fluorouracil with oxaliplatin (FOLFOX). In this group, the incidence of death decreased only when chemotherapy duration was more than 14 weeks (HR = 0.78, 95% CI: 0.630.97). A stronger association between adjuvant chemotherapy duration and postoperative death was detected in the capecitabine and oxaliplatin (CAPOX) group (HR = 0.71, 95% CI: 0.55–0.94) compared with the FOLFOX group (HR = 0.96, 95% CI: 0.92–1.01).ConclusionsThe duration of adjuvant chemotherapy with the FOLFOX regimen is associated with a non-linear reduction in postoperative deaths, which is only apparent after at least 14 weeks of treatment. However, patients with colorectal cancer may receive CAPOX adjuvant treatment for a longer duration.
Unprovoked pulmonary embolism in older adults: incidence and prognosis
Authors: Aharoni Mor , Horesh Nir , Rogowski Ori , Kremer Anjelika , Mayan Haim , Justo Dan 
Published: March 2021

IntroductionUnprovoked pulmonary embolism (UPE) is not rare and it is associated with an unfavorable prognosis in adults. However, the incidence and the prognosis of UPE in older adults have never been studied.Material and methodsThis was a historical prospective study. We reviewed all the medical charts of all older adults (aged 70 years or more) with UPE, provoked pulmonary embolism (PPE), and malignancy-associated PE (MAPE), admitted to a tertiary medical center between 2010 and 2012. The all-cause 3-year mortality rates and cumulative survival following admission were compared between the groups.ResultsThe final cohort included 249 patients with PE: 161 (64.7%) were women; the mean age was 79.8 ±5.7 years. Overall, 36 (14.5%) patients had UPE, 81 (32.5%) patients had MAPE, and 132 (53.0%) patients had PPE. Overall, 39 (15.7%) patients died within 30 days, 76 (30.5%) patients died within 6 months, 101 (40.6%) patients died within 1 year, and 136 (54.6%) patients died within 3 years of admission. Relative to PPE and MAPE patients, the cumulative survival was significantly higher in UPE patients at each time point within 1 year of admission (p < 0.05 and p < 0.001, respectively). However, 3 years after admission, the cumulative survival was comparable between PPE patients and UPE patients, and was significantly lower in MAPE patients (p < 0.001).ConclusionsUPE is not rare in older adults with PE, and it is associated with a favorable prognosis within 1 year of admission in this population.
MODELAGEM DAS VARIÁVEIS DO ENSAIO DE DESGASTE DO REVESTIMENTO DE CARBONETO DE CROMO
Authors: Alves Manoel Henrique , Alves Annelise Kopp 
Published: March 2021

Este trabalho tem como objetivo avaliar numericamente a taxa média de desgaste do revestimento de carboneto de cromo na matriz NiCr aspergido via HVOF em aço, através da regressão multivariada. A modelagem é realizada através de variáveis importantes de entrada no mecanismo de desgaste, a carga estática, velocidade e distância de deslizamento. Para isso foi feito uma pesquisa em artigos publicados em revistas internacionais para formar um banco de dados sobre esse revestimento, com dados experimentais já realizados. Com isso é modelado uma previsão da taxa média de desgaste do revestimento de acordo com tais variáveis gerando uma equação previsora. A modelagem se mostrou forte numericamente para prever o quanto o revestimento vai se desgastar. Pode-se verificar que a carga estática é uma variável determinante na previsão da taxa média de desgaste, sendo uma grandeza diretamente proporcional.
Kecerdasan Spiritual Berkorelasi dengan Tingkat Kecemasan Pasien Gagal Ginjal Kronik yang Menjalani Hemodialisa di Ruang Hemodialisa RSUD dr. M. HAULUSSY Ambon
Authors: Sumah Dene Fries 
Published: March 2021

Gagal Ginjal Kronik adalah gangguan fungsi ginjal yang progresif dan tidak dapat pulih kembali, dimana tubuh tidak mampu memelihara metabolisme, gagal memelihara keseimbangan cairan dan elektrolit yang berakibat pada peningkatan ureum. Pasien gagal ginjal kronis yang menjalani hemodialisis akan mengalami kecemasan yang disebabkan oleh berbagai stressor, diantaranya pengalaman nyeri pada daerah penusukan saat memulai hemodialysis. Tujuan dari penelitian ini adalah menganalisa hubungan kecerdasan spiritual dengan tingkat kecemasan pasien hemodialisis di ruang hemodialisis RSUD dr. M. Haulussy Ambon. Desain penelitian ini merupakan deskriptif Analitik dengan pendekatan cross sectional. Teknik pengambilan sampel adalah purposive sampling yang melibatkan 63 responden. Instrument penelitian menggunakan kuesioner untuk kecerdasan spiritual yang diadopsi dari penelitian Nurul Qomariah dan kuesioner kecemasan Zung Self-Rating Anxiety Scale dan modifikasi dengan kuesioner Hamilton Anxiety Range Of Scale menggunakan uji statistik chi-square dengan tingkat kemaknaan a = 0,05. Hasil analisis Chi Square terdapat hubungan yang bermakna antara kecerdasan spiritual dengan tingkat kecemasan pasien gagal ginjal kronik yang menjalani hemodialisa dengan nilai signifikan (p-value) adalah 0,000 (<0,05). Kesimpulan Ada hubungan yang bermakna antara kecerdasan spiritual dengan tingkat kecemasan pasien gagal ginjal kronik yang menjalani hemodialisa di RSUD dr. M. Haulussy Ambon. Saran bagi pasien diharapkan agar dapat mengembangkan kecerdasan spiritual dengan cara mengikuti kegiatan-kegiatan ibadah sehingga kecemasan saat menjalani hemodialisa berkurang.
Determinan Keputusan Rasional Menjadi Wajib Pajak Prioritas
Authors: Nur Muhammad , Masud Masdar , Mursalim Mursalim 
Published: March 2021

Penelitian ini bertujuan untuk menganalisis dan mengkaji pengaruh kualitas pelayanan, sanksi perpajakan, kecerdasan interpersonal dan reward terhadap keputusan rasional wajib pajak dengan status prioritas pada Kantor Pelayanan Pajak Pratama Makassar Barat. Metode penelitian yang digunakan dalam penelitian ini adalah Analisis deskriptif. jumlah sampel sebanyak 67 responden. Berdasarkan hasil analisis regresi linier berganda. Hasil penelitian ini menemukan bahwa kualitas pelayanan, kecerdasan interpersonal dan reward berpengaruh positif dan signifikan terhadap keputusan rasional wajib dengan dengan status prioritas sedangkan Sanksi perpajakan berpengaruh positif namun tidak signifikan terhadap keputusan wajib pajak dengan status prioritas.
This study aims to analyze and examine the effect of service quality, tax sanctions, interpersonal intelligence and reward on rational decisions of taxpayers with priority status at the West Makassar Pratama Tax Office. The research method used in this research is descriptive analysis. the number of samples was 67 respondents. Based on the results of multiple linear regression analysis. The results of this study found that service quality, interpersonal intelligence and reward had a positive and significant effect on mandatory rational decisions with priority status while tax sanctions had a positive but not significant effect on taxpayer decisions with priority status.
Pembuktian Perkara Tindak Pidana Korupsi Yang Diputus Bebas: Studi Pengadilan Negeri Makassar
Authors: Budiman Budiman , Thalib Hambali , Ahmad Kamri 
Published: March 2021

Tujuan penelitan ini adalah untuk 1) untuk mengetahui pertimbangan Majelis Hakim dama penilaian sah tidaknya alat bukti dalam menjatuhkan putusan bebas terhadap tindak pidana korupsi. 2) untuk mengetahui putusan bebas terhadap yang dijatuhkan oleh Majelis Hakim dan tindak pidana korupsi sesuai dengan ketentuan hukum pidana materil dan hukum pidana formil. Penelitian ini merupakan penelitian normatif yaitu dengan mengkaji putusan besar diputuskan oleh majelis hakim di pengadilan. Hasil penelitan menunjukan bahwa pembahasan terhadap putusan bebas perkara tindak pidana korupsi Pengadilan Negri Makassar hakim dalam menjatuhkan Putusan bebas terhadap tindak pidana korupsi dengan pertimbangannya tergantung kesesuaian antara keterangan saksi dan alat bukti, serta keluarga ahli. 3) bahwa putusan bebas yang diputuskan oleh Majelis Hakim tindak pidana materil dan hukum formil.
The purpose of this research is to 1) to find out that the Judges' assessment is invalid as evidence in issuing a free decision against a criminal act of corruption. 2) to find out the verdicts free from those imposed by the Panel of Judges and corruption in accordance with the provisions of material law and formal criminal law. This research is a normative study that examines major decisions made by judges in court. The results of the research refer to the discussion of the case-free verdict in the Negri Realtor Court 3) that the acquittal is decided by the Panel of Judges for material and formal criminal acts.