bluuebunny/crossref_metadata_2025_split huggingface dataset:
Digital pathology in the immunohistochemical evaluation of biomarkers in breast cancer
Authors: Lopes J , Silva S , Monteiro J , Pinto I M , Silva R A 
Published: August 2021

Abstract

Background
Currently, the evaluation of biomarkers HER-2 and ER is critical for targeting therapy for breast cancer and is performed by experienced pathologists, which takes time and causes a certain interobserver variability. The use of histological slide scanners coupled with the application of software for immunoexpression quantification can be profitable in pathological laboratories routine. This study aims to compare the quantification of HER-2 and ER immunoexpression employing automatic algorithms, using as standard the evaluation performed by a pathologist.

Methods
From the archive since 2016 to 2018 were retrieved 75 immunostained slides for HER-2 and 76 immunostained slides for ER with invasive breast carcinoma diagnosis. The slides were scanned in Aperio CS2 and the immunostaining signal was quantified in Aperio Imagescope using a membrane and nuclear algorithm, respectively. The concordance between the scores obtained and the previous assessment was calculated by Cohen’s Kappa coefficient as well as the sensitivity and specificity for each algorithm.

Results
Both algorithms showed an almost perfect concordance with the conventional method (k = 0.94 for HER-2; k = 0.92 for ER). Digital evaluations presented a sensitivity of 100% for both biomarkers, and a specificity of 100% for HER-2 and 80% for ER.

Conclusions
This assay showed that applying signal quantification software for HER-2 and ER in digitized slides is accurate, as these tools have potential to be implemented in laboratory routine. However, it will be necessary to increase the sample to obtain more reliable values and extend this study to the PR and Ki67 biomarkers since both carry prognostic and predictive information as well.
Evaluation of radiation dose in pediatric radiological exams
Authors: Vosiak Paula , Schelin Hugo Reuters , Bunick Ana Paula , Yagui Akemi , Legnani Adriano 
Published: August 2021

Abstract

Background
Pediatric patients are in a phase of high anatomical and physiological development, whose characteristics make them more radiosensitive to certain radiation exposures. Exposure to doses of radiation can lead to the occurrence of biological effects, including cancer.

Objectives
To analyze radiation exposures resulting from pediatric radiological examinations and to perform an optimization process, with the purpose to adequate use of the principles of radiological protection.

Methods
The analysis of radiation doses was performed using the technical parameters of the tests (voltage, current, exposure time and focus-skin distance), the patient's parameters (age, weight and thickness), the calculation of the radiation dose, using the output of the X-ray tube, all recorded at the time of the examination. The results were classified into 4 age groups and 5 weight groups.

Results
The results were classified according to the patient's age and weight, taking into account the radiation dose received. The data were also separated by gender, as the analysis showed that female patients are slightly smaller than male patients, and as a consequence, the doses received by this gender were lower. Examinations of the chest, abdomen, pelvis, skull and sinuses were analyzed and some groups received higher doses compared to other studies and international dose reference levels (DRL).

Conclusions
An optimization study must be carried out to verify the possible cause of such values, readjusting the values of the technical parameters and performing a new radiation dose analysis.
Preterm infants comfort before and after capillary blood sampling procedure
Authors: Balciunaite Karolina , Vaskelyte Alina 
Published: August 2021

Abstract

Background
Because of the immaturity and severe condition preterm infants undergoes a lot of painful and stressful procedures during their care that can cause short-term and long-term negative consequences. Maintenance of optimal comfort is essential for good clinical practice. To assess the comfort of preterm infants before and after the capillary blood sampling procedure.

Methods
The quantitative observational study was conducted in May 2019—February 2020 in the University hospital NICU. The research instrument was a neonatal comfort scale COMFORTneo, which total scores ranged 6–30. Higher scores indicated higher discomfort. Preterm infant comfort was assessed before the capillary blood sampling procedure and 30 min. after applying non-pharmacological pain relief and comfort assurance methods and implementing the procedure. The research was conducted in accordance with ethical principles.

Results
Comfort was assessed in 157 preterm newborns with a gestational age of 30 ± 3.3 weeks and a postnatal age of 6.0 ± 5.7 days. The mean value of neonatal comfort before the capillary blood sampling procedure was 10.0 ± 3.2 points, after the procedure 9.1 ± 2.4 points. A statistically significant inverse relationship was found between pre-procedure and post-procedure comfort (Z = -5.374; P &lt; 0.001). A statistically significant weak inverse relationship was found between gestational age and comfort scores before the procedure (r = -0.204; P = 0.01) and after the procedure (r = -0.321; P = 0.008).

Conclusions
In most cases, preterm infants were feeling moderate discomfort before and after the capillary blood sampling procedure. Newborns had more discomfort before the procedure than 30 min. after the completion of the procedure. Lower gestational age newborns were feeling greater discomfort.
Assessment of utilization and willingness to use it devices to support heath management
Authors: Espírito-Santo Margarida , Moura Maria Margarida Madeira , Lapão Nelson , Peixoto Stefany 
Published: August 2021

Abstract

Background
Amongst the technological solutions that aim to facilitate daily tasks, the potential contributions of mhealth is significant, mainly within the crescent elderly population presenting chronic diseases and using medication. Thus, this study aimed to assess the utilization and willingness to use IT devices to support health management, such as medication management.

Methods
A cross-sectional study was held, including the application (face-to-face and online) of an electronic questionnaire, with 164 participants. Data were processed using SPSS v27.

Results
Study participants had a mean age of 37.6 ± 20.0 years old, 23.8% were ≥ 60 years old, 73.2% female, 28% declared to suffer a chronic disease, with 27.4% using chronic medication and mostly managed by oneself (71.1%). Most of the participants owned and used a mobile phone (respectively 93.9% and 59.8%), a tablet (48.8% and 44.5%), and a computer (73.8% and 17.1%). Only about half of the participants aged ≥ 60 years declared to use their mobile devices to internet access (mobile phones 51.3%, tablet 35.9% and computers 41%). In this group, only 2% mentioned using applications in the health area. Overall, 85.4% considered these tools could be useful, although fewer would be willing to buy them (33.6% for &lt; 60 years and 66.7% for ≥ 60 years).

Conclusions
The use of IT devices is less widespread in the older population, the age groups with greatest need for tools to support health management. Future development of this area must pay special attention to target audience characteristics and their limitations in the use of technology.
Family influence on perinatal depression
Authors: Saraiva Ana , Rosendo Inês , Miguel Susana , Duarte Joana , Marques Tiago , Baptista Sylvie 
Published: August 2021

Abstract

Background
The perinatal period is of big changes and adaptations for pregnant woman, making them more susceptible to depression. Perinatal depression can have huge negative consequences for the pregnant woman, child, family and society. Thus, the objective of this work is to understand the family influence in the appearance of this pathology.

Methods
This is a cross-sectional observational study of a sample of pregnant women included for convenience, who answered a questionnaire with sociodemographic questions, pathological and physiological background and 4 scales: ERAP, EADS-21, FACES IV and ESSS. A descriptive analysis was carried out and then an inferential statistical analysis between the levels of depression, stress and anxiety and different variables.

Results
The sample includes 41 pregnant women who agreed to participate in the study. It was found that only the family variables related to ‘Satisfaction with the family’ (P = 0.024) and ‘Intimacy’ (P = 0.006) seem to have significant relationship with the levels of perinatal depression. As possible confounding variables influencing these levels, we have the fact that pregnancy is planned (P = 0.049), ‘Satisfaction with friends’ (P = 0.001) and ‘Social activities’ (P≤0.001).

Conclusions
It was proven that high levels of satisfaction with family and intimacy are protective factors for the development of perinatal depression. These family-related risk factors can be easily addressed in the context of family’s doctor appointments and help to detect pregnant women at risk of perinatal depression. Deepening this knowledge will make it possible to prevent and intervene earlier in these cases and, consequently, reduce the harmful effects of this pathology.
In vitro characterization of Pseudomonas aeruginosa recovered in Portugal from low respiratory tract infections in ICU patients (STEP Study)
Authors: Hernández-García Marta , García-Fernández Sergio , García-Castillo María , Pássaro Leonor , Cantón Rafael ,   STEP study group, Melo-Cristino José , Pinto Margarida F , Marcelo Cristina , Peres Helena , Lourenço Isabel , Peres Isabel , Marques João , Chantre Odete , Pina Teresa , Microbiologia Laboratório de , Clínica Serviço de Patologia , Gonçalves Elsa , Toscano Cristina , Alves Valquíria , Microbiologia Serviço de , Ribeiro Manuela , Costa Eliana , Vieira Ana Raquel , Clínica Serviço Patologia , Ferreira Sónia , Diaz Raquel , Ramalheira Elmano , Clínica Serviço Patologia , Schäfer Sandra , Tancredo Luísa , Sancho Luísa , Clínica Serviço de Patologia , Rodrigues Ana , Diogo José , Microbiologia Serviço de , Ferreira Rui , Ramos Helena , Silva Tânia , Silva Daniela , Microbiologia Serviço de , Chaves Catarina , Queiroz Carolina , Nabiev Altair , Microbiologia Serviço de , Pássaro Leonor , Paixao Laura , Romano João , Moura Carolina , Castillo María García del , García-Fernández Sergio , Hernández-García Marta , Cantón Rafael 
Published: August 2021

ABSTRACT
Purpose: to characterize the distribution and mechanisms involved in ceftolozane/tazobactam (C/T) resistance in Pseudomonas aeruginosa isolates recovered from intensive care units (ICUs) in Portugal as part of the STEP surveillance study. Materials and methods: a total of 226 P. aeruginosa isolates were collected from patients with low respiratory tract infections (LRTI) admitted to ICUs between June 2017 and July 2018. Susceptibility to antimicrobials including the recent C/T combination was determined by EUCAST-criteria. Whole genome sequencing (WGS) was performed in a subset of 17 isolates. Results: multidrug resistant (MDR) and extremely drug resistant (XDR) phenotypes accounted for 20.4% and 25.7% of cases, respectively. C/T showed the highest susceptibility rate in both MDR (100%) and XDR (93.1%) isolates, followed by amikacin (97.8% MDR and 79.3% XDR). blaKPC-3 (n = 2) and blaGES-13 (n = 1) carbapenemase genes were detected in 3 of the 17 sequenced isolates, but only the GES-13-producing isolate displayed resistance to C/T. Additionally, the C/T-resistant phenotype was also found in two non-carbapenemase producers that carried known ceftolozane/tazobactam resistance-associated mutations in the PBP3 gene. Conclusions: C/T was highly active against MDR/XDR-P. aeruginosa isolates causing LRTI in ICUs. Moreover, beyond carbapenemase-encoding genes, mutations in chromosomal PBP-encoding genes might also be involved in ceftolozane/tazobactam resistance in Portugal.
The Evolution of Comparative Phylogeography: Putting the Geography (and More) into Comparative Population Genomics
Authors: Edwards Scott V , Robin V V , Ferrand Nuno , Moritz Craig 
Published: August 2021

AbstractComparative population genomics is an ascendant field using genomic comparisons between species to draw inferences about forces regulating genetic variation. Comparative phylogeography, by contrast, focuses on the shared lineage histories of species codistributed geographically and is decidedly organismal in perspective. Comparative phylogeography is approximately 35 years old, and, by some metrics, is showing signs of reduced growth. Here, we contrast the goals and methods of comparative population genomics and comparative phylogeography and argue that comparative phylogeography offers an important perspective on evolutionary history that succeeds in integrating genomics with landscape evolution in ways that complement the suprageographic perspective of comparative population genomics. Focusing primarily on terrestrial vertebrates, we review the history of comparative phylogeography, its milestones and ongoing conceptual innovations, its increasingly global focus, and its status as a bridge between landscape genomics and the process of speciation. We also argue that, as a science with a strong “sense of place,” comparative phylogeography offers abundant “place-based” educational opportunities with its focus on geography and natural history, as well as opportunities for collaboration with local communities and indigenous peoples. Although comparative phylogeography does not yet require whole-genome sequencing for many of its goals, we conclude that it nonetheless plays an important role in grounding our interpretation of genetic variation in the fundamentals of geography and Earth history.
A Framework for Investigating Rules of Life Across Disciplines
Authors: Poust Ashley W , Bogar Laura , Robinson W Douglas , Poole Geoffrey 
Published: August 2021

Synopsis
Clearly and usefully defining the Rules of Life has long been an attractive yet elusive prospect for biologists. Life persists because requirements for existence and successful transmission of hereditary information are met. These requirements are met through mechanisms adopted by organisms, which produce solutions to environmentally imposed constraints on life. Yet, constraints and their suites of potential solutions are typically context-specific, operating at specific levels of organization, or holons, and having cascading effects across multiple levels, or the holarchy. We explore the idea that the interaction of constraints, mechanisms, and requirements within and across levels of organization may produce rules of life that can be productively defined. Although we stop short of listing specific rules, we provide a conceptual framework within which progress towards identifying rules might be made.
The Gut Microbiome of Healthy Vietnamese Adults and Children Is a Major Reservoir for Resistance Genes Against Critical Antimicrobials
Authors: Pereira-Dias Joana , Nguyen Ngoc Minh Chau , Tran Thi Hong Chau , Nguyen Thi Nguyen To , Ha Thanh Tuyen , Zellmer Caroline , Chung The Hao , Pike Lindsay , Higginson Ellen E , Baker Stephen 
Published: August 2021

Abstract
Antimicrobials are a key group of therapeutic agents. Given the animal/human population density and high antimicrobial consumption rate in Southeast Asia, the region is a focal area for monitoring antimicrobial resistance (AMR). Hypothesizing that the gastrointestinal tract of healthy individuals in Vietnam is a major source of AMR genes that may be transferred to pathogens, we performed shotgun metagenomic sequencing on fecal samples from 42 healthy Vietnamese people (21 children and 21 adults). We compared their microbiome profiles by age group and determined the composition of AMR genes. An analysis of the taxonomic profiles in the gut microbiome showed a clear differentiation by age, with young children (age &lt;2 years) exhibiting a unique structure in comparison to adults and older children. We identified a total of 132 unique AMR genes, with macrolide, lincosamide, and streptogramin class resistance genes (ermB and lnuC) and tetracycline resistance genes being almost ubiquitous across the study population. Notably, samples from younger children were significantly associated with a greater number of AMR genes than other age groups, including key signature genes associated with AMR pathogens (eg, blaCTX-M, mphA). Our data suggest that the gut microbiome of those living in Vietnam, particularly young children, is a substantial reservoir of AMR genes, which can be transferred to circulating enteric pathogens. Our data support the generation of longitudinal cohort studies of those living in urban and rural areas of developing countries to understand the behavior of these AMR reservoirs and their role in generating multidrug-resistant and extensively drug-resistant pathogens.
Some thoughts about the Intersection between Data Protection and Competition Law: A View from Brazil
Authors: Macedo Alexandre Cordeiro 
Published: August 2021

Abstract
This article presents some thoughts about the relationship between Data Protection and Competition Law—focusing on the implementation of the New Brazilian Data Protection Law (LGPD). The Digital Era, the Digital Economy, and the Data-Driven Market (although they present different definitions, they are all concepts that have a strong connection between their meanings) are significantly changing a variety of aspects in our lives. In terms of legal consequences, the Digital Era has created a new, unique and specialized field (Data Protection). Although Data Protection is affecting Competition Law, as well as other fields of law, it is always important to have in mind its purposes and goals in order to not merge what should not be merged.
Assessing the Single and Combined Toxicity of the Bioinsecticide Spear and Cry3Bb1 Protein Against Susceptible and Resistant Western Corn Rootworm Larvae (Coleoptera: Chrysomelidae)
Authors: Pereira Adriano E , Huynh Man P , Carlson Alvar R , Haase Alexandra , Kennedy Robert M , Shelby Kent S , Coudron Thomas A , Hibbard Bruce E 
Published: August 2021

Abstract
The western corn rootworm (WCR), Diabrotica virgifera virgifera LeConte (Coleoptera: Chrysomelidae), poses a serious threat to maize (Zea mays L.) growers in the U.S. Corn Belt. Transgenic corn expressing Bacillus thuringiensis (Bt) Berliner is the major management tactic along with crop rotation. Bt crops targeting WCR populations have been widely planted throughout the Corn Belt. Rootworms have developed resistance to nearly all management strategies including Bt corn. Therefore, there is a need for new products that are not cross-resistant with the current Bt proteins. In this study, we evaluated the susceptibility of WCR strains resistant and susceptible to Cry3Bb1 to the biological insecticide Spear-T (GS-omega/kappa-Hexatoxin-Hv1a) alone and combined with Cry3Bb1 protein. The activity of Hv1a alone was similar between Cry3Bb1-resistant and susceptible strains (LC50s = 0.95 mg/cm2 and 1.50 mg/cm2, respectively), suggesting that there is no cross-resistance with Cry3Bb1 protein. Effective concentration (EC50), molt inhibition concentration (MIC50), and inhibition concentration (IC50) values of Hv1a alone were also similar between both strains, based on non-overlapping confidence intervals. Increased mortality (64%) was observed on resistant larvae exposed to Hv1a (0.6 mg/cm2) + Cry3Bb1 protein (170.8 µg/cm2) compared to 0% mortality when exposed to Cry3Bb1 alone and 34% mortality to Hv1a alone (0.3 mg/cm2). The time of larval death was not significantly different between Hv1a alone (3.79 mg/cm2) and Hv1a (0.6 mg/cm2) + Cry3Bb1 (170.8 µg/cm2). New control strategies that are not cross-resistant with current insecticides and Bt proteins are needed to better manage the WCR, and Hv1a together with Cry3Bb1 may fit this role.
Highlights of Medical Entomology, 2020
Authors: Stone Chris M 
Published: August 2021

Abstract
Medical Entomology as a field is inherently global – thriving on international and interdisciplinary collaborations and affected dramatically by arthropod and pathogen invasions and introductions. This past year also will be remembered as the year in which the SARS-CoV-2 COVID-19 pandemic affected every part of our lives and professional activities and impacted (or changed, sometimes in good ways) our ability to collaborate and detect or respond to invasions. This incredible year is the backdrop for the 2020 Highlights in Medical Entomology. This article highlights the broad scope of approaches and disciplines represented in the 2020 published literature, ranging from sensory and chemical ecology, population genetics, impacts of human-mediated environmental change on vector ecology, life history and the evolution of vector behaviors, to the latest developments in vector surveillance and control.
The proper motion of Andromeda from Gaia EDR3: confirming a nearly radial orbit
Authors: Salomon J-B , Ibata R , Reylé C , Famaey B , Libeskind N I , McConnachie A W , Hoffman Y 
Published: August 2021

Abstract
We present an analysis of the proper motion of the Andromeda galaxy (M31), based on the Early Third Data Release of the Gaia mission. We use the Gaia photometry to select young blue main sequence stars, and apply several quality cuts to obtain clean samples of these tracers. After correcting the proper motion measurements for the internal rotation of the M31 disk motion, we derive an apparent motion of 52.5 ± 5.8 μas/yr with respect to the Gaia reference frame, or 61.9 ± 9.7 μas/yr after applying a zero-point correction determined from quasars within $20\deg$ from M31 and a correction from systemic biases. Accounting for the Solar reflex motion we deduce a relative velocity between Andromeda and the Milky way (in a non-rotating frame at the current location of the Sun) of 42.2 ± 39.3 km s−1 along right ascension (40.0 ± 39.3 km s−1 along galactic longitude) and −59.4 ± 30.3 km s−1 along declination (−60.9 ± 30.3 km s−1 along galactic latitude), with a total transverse velocity of Vtrans = 82.4 ± 31.2 km s−1. These values are consistent with (but more accurate than) earlier Hubble Space Telescope measurements that predict a future merger between the two galaxies. We also note a surprisingly large difference in the derived proper motion between the blue stars in M31 and samples of red stars that appear to lie in that galaxy. We propose several hypotheses to explain the discrepancy but found no clear evidence with the current data to privilege any one of them.
Interconversion mechanisms of PN and PO in the interstellar medium through simple atom–diatom collisions
Authors: Souza André C , Silva Mateus X , Galvão Breno R L 
Published: August 2021

ABSTRACT
Models for the abundances of phosphorus-bearing molecules, such as PO and PN, rely on rate coefficients guessed from similar reactions of NO. Given the importance of these molecules in pre-biotic chemistry, in this work we carry out accurate multireference configuration interaction calculations on the N+PO and O+PN reactions, unravelling their underlying mechanisms and potential energy barriers. The collisions may occur in two triplet electronic states and involve six potential wells lying under the N+PO limit. The interaction between atomic nitrogen and PO is shown to occur barrierlessly only when dynamical correlation is included in the calculations. The theoretical results confirm the assumptions used in the astrochemical models that depletion of PO by nitrogen atoms occurs fast, with a branching ratio largely favouring O+PN over the more exothermic P+NO. Among other results, we predict that PN should be stable with respect to collisions with oxygen atoms under low-temperature environments, but will be depleted to P + NO in shocks and other conditions with higher translational energies.