bluuebunny/crossref_metadata_2025_split huggingface dataset:
An ALE meta-analytic review of top-down and bottom-up processing of music in the brain
Authors: Pando-Naude Victor , Patyczek Agata , Bonetti Leonardo , Vuust Peter 
Published: October 2021

AbstractA remarkable feature of the human brain is its ability to integrate information from the environment with internally generated content. The integration of top-down and bottom-up processes during complex multi-modal human activities, however, is yet to be fully understood. Music provides an excellent model for understanding this since music listening leads to the urge to move, and music making entails both playing and listening at the same time (i.e., audio-motor coupling). Here, we conducted activation likelihood estimation (ALE) meta-analyses of 130 neuroimaging studies of music perception, production and imagery, with 2660 foci, 139 experiments, and 2516 participants. We found that music perception and production rely on auditory cortices and sensorimotor cortices, while music imagery recruits distinct parietal regions. This indicates that the brain requires different structures to process similar information which is made available either by an interaction with the environment (i.e., bottom-up) or by internally generated content (i.e., top-down).
The early maternal environment shapes the parental response to offspring UV ornamentation
Authors: García-Campa Jorge , Müller Wendt , Hernández-Correas Ester , Morales Judith 
Published: October 2021

AbstractParents allocate resources to offspring to increase their survival and to maximize their own fitness, while this investment implies costs to their condition and future reproduction. Parents are hence expected to optimally allocate their resources. They should invest equally in all their offspring under good conditions, but when parental capacity is limited, parents should invest in the offspring with the highest probability of survival. Such parental favouritism is facilitated by the fact that offspring have evolved condition-dependent traits to signal their quality to parents. In this study we explore whether the parental response to an offspring quality signal depends on the intrinsic capacity of the parents, here the female. We first manipulated the intrinsic capacity of blue tit (Cyanistes caeruleus) females through lutein-supplementation during egg laying, and we subsequently blocked the UV/yellow reflectance of breast feathers on half of the nestlings in each brood. We did not find evidence that the female intrinsic capacity shaped parental feeding or sibling competition according to offspring UV/yellow colouration. However, nestling UV/yellow colour affected costly behavioural interactions in the form of prey-testings (when a parent places a prey item into a nestling’s gape but removes it again). In lutein-supplemented nests, fathers but not mothers favoured UV-blocked chicks by testing them less often, supporting previous results. Accordingly, in lutein-supplemented nests, UV-blocked nestlings gained more mass than their siblings, while in control nests we found the opposite effect and UV-blocked nestlings gained less. Our results emphasize that the prenatal environment shaped the role of offspring UV/yellow colour during certain family interactions and are indicative for sex-specific parental care strategies.
High canopy cover of invasive Acer negundo L. affects ground vegetation taxonomic richness
Authors: Veselkin D. V. , Dubrovin D. I. , Pustovalova L. A. 
Published: October 2021

AbstractWe assessed the link between canopy cover degree and ground vegetation taxonomic richness under alien ash-leaved maple (Acer negundo) and other (native or alien) tree species. We investigated urban and suburban forests in the large city of Yekaterinburg, Russia. Forests were evaluated on two spatial scales. Through an inter-habitat comparison we recorded canopy cover and plant taxonomic richness among 13 sample plots of 20 × 20 m where A.negundo dominated and 13 plots where other tree species dominated. In an intra-habitat comparison, we recorded canopy cover and ground vegetation taxonomic richness among 800 sample plots measuring 1 m2in the extended urbanised forest, which featured abundant alien (308 plots) and native trees (492 plots). We observed decreased taxonomic richness among vascular ground plant species by 40% (inter-habitat) and 20% (intra-habitat) in areas dominated byA. negundocompared to areas dominated by native tree and shrub species. An abundance of A. negundo was accompanied by increased canopy cover. We found a negative relationship between canopy cover and the number of understory herbaceous species. Thus, the interception of light and the restriction of its amount for other species is a main factor supporting the negative influence of A.negundo on native plant communities.
Interspecific variation in evaporative water loss and temperature response, but not metabolic rate, among hibernating bats
Authors: McGuire Liam P. , Fuller Nathan W. , Dzal Yvonne A. , Haase Catherine G. , Klüg-Baerwald Brandon J. , Silas Kirk A. , Plowright Raina K. , Lausen Cori L. , Willis Craig K. R. , Olson Sarah H. 
Published: October 2021

AbstractHibernation is widespread among mammals in a variety of environmental contexts. However, few experimental studies consider interspecific comparisons, which may provide insight into general patterns of hibernation strategies. We studied 13 species of free-living bats, including populations spread over thousands of kilometers and diverse habitats. We measured torpid metabolic rate (TMR) and evaporative water loss (two key parameters for understanding hibernation energetics) across a range of temperatures. There was no difference in minimum TMR among species (i.e., all species achieved similarly low torpid metabolic rate) but the temperature associated with minimum TMR varied among species. The minimum defended temperature (temperature below which TMR increased) varied from 8 °C to < 2 °C among species. Conversely, evaporative water loss varied among species, with species clustered in two groups representing high and low evaporative water loss. Notably, species that have suffered population declines due to white-nose syndrome fall in the high evaporative water loss group and less affected species in the low evaporative water loss group. Documenting general patterns of physiological diversity, and associated ecological implications, contributes to broader understanding of biodiversity, and may help predict which species are at greater risk of environmental and anthropogenic stressors.
Simple urine storage protocol for extracellular vesicle proteomics compatible with at-home self-sampling
Authors: Erozenci L. A. , Pham T. V. , Piersma S. R. , Dits N. F. J. , Jenster G. W. , van Royen M. E. , Moorselaar R. J. A. , Jimenez C. R. , Bijnsdorp I. V. 
Published: October 2021

AbstractUrinary extracellular vesicles (EVs) have gained increased interest as a biomarker source. Clinical implementation on a daily basis requires protocols that inevitably includes short-term storage of the clinical samples, especially when collected at home. However, little is known about the effect of delayed processing on the urinary EVs concentration and proteome. We evaluated two storage protocols. First, urine stored at 4 °C. Secondly a protocol compatible with at-home collection, in which urine was stored with the preservative EDTA at room temperature (RT). EVs were isolated using the ME-kit (VN96-peptide). For both conditions we explored the effect of storage duration (0, 2, 4 and 8 days) on EV concentration and proteome using EVQuant and data-independent acquisition mass spectrometry, respectively. The urinary EV concentration and proteome was highly stable using both protocols, in terms of protein number and quantitative changes. Furthermore, EDTA does not affect the urinary EV concentration or global proteome. In conclusion, urine can be stored either at 4 °C or with EDTA at RT for up to 8 days without any significant decay in EV concentration or a notable effect on the EV-proteome. These findings open up biomarker studies in urine collected via self-sampling at home.
De novo electrocardiographic abnormalities in persons living with HIV
Authors: Knudsen Andreas D. , Graff Claus , Nielsen Jonas Bille , Thomsen Magda Teresa , Høgh Julie , Benfield Thomas , Gerstoft Jan , Køber Lars , Kofoed Klaus F. , Nielsen Susanne D. 
Published: October 2021

AbstractPersons living with HIV (PLWH) may have increased incidence of cardiovascular events and longer QTc intervals than uninfected persons. We aimed to investigate the incidence and risk factors of de novo major electrocardiogram (ECG) abnormalities and QTc prolongation in well-treated PLWH. We included virologically suppressed PLWH without major ECG abnormalities, who attended the 2-year follow-up in the Copenhagen comorbidity in HIV infection (COCOMO) study. ECGs were categorized according to Minnesota Code Manual. We defined de novo major ECG abnormalities as new major Minnesota Code Manual abnormalities. Prolonged QTc was defined as QTc > 460 ms in females and QTc > 450 ms in males. Of 667 PLWH without major ECG abnormalities at baseline, 34 (5%) developed de novo major ECG abnormalities after a median of 2.3 years. After adjustment, age (RR: 1.57 [1.08–2.28] per decade older), being underweight (RR: 5.79 [1.70–19.71]), current smoking (RR: 2.34 [1.06–5.16]), diabetes (RR: 3.89 [1.72–8.80]) and protease inhibitor use (RR: 2.45 [1.27–4.74) were associated with higher risk of getting de novo major ECG abnormalities. Of PLWH without prolonged QTc at baseline, only 11 (1.6%) participants developed de novo prolonged QTc. Five percent of well-treated PLWH acquired de novo major ECG abnormalities and protease inhibitor use was associated with more than twice the risk of de novo major ECG abnormalities. De novo prolonged QTc was rare and did not seem to constitute a problem in well-treated PLWH.
Quantum chemical insight into the effects of the local electron environment on T2*-based MRI
Authors: Petronek M. S. , St-Aubin J. J. , Lee C. Y. , Spitz D. R. , Gillan E. G. , Allen B. G. , Magnotta V. A. 
Published: October 2021

AbstractT2* relaxation is an intrinsic magnetic resonance imaging (MRI) parameter that is sensitive to local magnetic field inhomogeneities created by the deposition of endogenous paramagnetic material (e.g. iron). Recent studies suggest that T2* mapping is sensitive to iron oxidation state. In this study, we evaluate the spin state-dependence of T2* relaxation using T2* mapping. We experimentally tested this physical principle using a series of phantom experiments showing that T2* relaxation times are directly proportional to the spin magnetic moment of different transition metals along with their associated magnetic susceptibility. We previously showed that T2* relaxation time can detect the oxidation of Fe2+. In this paper, we demonstrate that T2* relaxation times are significantly longer for the diamagnetic, d10 metal Ga3+, compared to the paramagnetic, d5 metal Fe3+. We also show in a cell culture model that cells supplemented with Ga3+ (S = 0) have a significantly longer relaxation time compared to cells supplemented with Fe3+ (S = 5/2). These data support the hypothesis that dipole–dipole interactions between protons and electrons are driven by the strength of the electron spin magnetic moment in the surrounding environment giving rise to T2* relaxation.
Expectations of objective threats and aversive feelings in specific fears
Authors: Elsey James W. B. , Kindt Merel 
Published: October 2021

AbstractMistaken beliefs about danger posed by feared stimuli are considered a key factor causing and maintaining fears. Such beliefs are intriguing because many fearful people express them, but acknowledge they are untrue in reality. While previous research indicates fearful individuals may not wholly endorse their beliefs about objective threats (e.g., the spider will bite), expectations of negative subjective consequences (e.g., I will feel terrible) are also likely to be important. We investigated the extent to which participants’ expectations of objective and subjective threats were sensitive to manipulations that encouraged them to consider whether their expectations were likely to happen in reality. Across five online experiments (N = 560, or 727 with more liberal inclusion criteria), such manipulations produced lower expectancy ratings for objective but not subjective threats (versus participants who gave ratings without the manipulation). Most participants reported that anticipation of negative feelings was more concerning than actual danger. Hence, numerous fear-relevant expectations about objective threat—considered central in understanding why people are irrationally afraid—respond to small cognitive manipulations. Additionally, expectations of negative subjective experiences during fear-provoking encounters appear to be more consistently endorsed, and feature prominently in fearful individuals’ concerns about what will happen in a fear-relevant situation.
The nomogram based on the 6-lncRNA model can promote the prognosis prediction of patients with breast invasive carcinoma
Authors: Luo Dankun , Yao Wenchao , Wang Qiang , Yang Qiu , Liu Xuxu , Yang Yang , Zhang Weihui , Xue Dongbo , Ma Biao 
Published: October 2021

AbstractLong non-coding RNA (lncRNA) is a prognostic biomarker for many types of cancer. Here, we aimed to study the prognostic value of lncRNA in Breast Invasive Carcinoma (BRCA). We downloaded expression profiles from The Cancer Genome Atlas (TCGA) datasets. Subsequently, we screened the differentially expressed genes between normal tissues and tumor tissues. Univariate Cox, LASSO regression, and multivariate Cox regression analysis were used to construct a lncRNA prognostic model. Finally, a nomogram based on the lncRNAs model was developed, and weighted gene co-expression network analysis (WGCNA) was used to predict mRNAs related to the model, and to perform function and pathway enrichment. We constructed a 6-lncRNA prognostic model. Univariate and multivariate Cox regression analysis showed that the 6-lncRNA model could be used as an independent prognostic factor for BRCA patients. We developed a nomogram based on the lncRNAs model and age, and showed good performance in predicting the survival rates of BRCA patients. Also, functional pathway enrichment analysis showed that genes related to the model were enriched in cell cycle-related pathways. Tumor immune infiltration analysis showed that the types of immune cells and their expression levels in the high-risk group were significantly different from those in the low-risk group. In general, the 6-lncRNA prognostic model and nomogram could be used as a practical and reliable prognostic tool for invasive breast cancer.
Shotgun-metagenomics based prediction of antibiotic resistance and virulence determinants in Staphylococcus aureus from periprosthetic tissue on blood culture bottles
Authors: Sanabria Adriana Maria , Janice Jessin , Hjerde Erik , Simonsen Gunnar Skov , Hanssen Anne-Merethe 
Published: October 2021

AbstractShotgun-metagenomics may give valuable clinical information beyond the detection of potential pathogen(s). Identification of antimicrobial resistance (AMR), virulence genes and typing directly from clinical samples has been limited due to challenges arising from incomplete genome coverage. We assessed the performance of shotgun-metagenomics on positive blood culture bottles (n = 19) with periprosthetic tissue for typing and prediction of AMR and virulence profiles in Staphylococcus aureus. We used different approaches to determine if sequence data from reads provides more information than from assembled contigs. Only 0.18% of total reads was derived from human DNA. Shotgun-metagenomics results and conventional method results were consistent in detecting S. aureus in all samples. AMR and known periprosthetic joint infection virulence genes were predicted from S. aureus. Mean coverage depth, when predicting AMR genes was 209 ×. Resistance phenotypes could be explained by genes predicted in the sample in most of the cases. The choice of bioinformatic data analysis approach clearly influenced the results, i.e. read-based analysis was more accurate for pathogen identification, while contigs seemed better for AMR profiling. Our study demonstrates high genome coverage and potential for typing and prediction of AMR and virulence profiles in S. aureus from shotgun-metagenomics data.
The technology of artificial pneumoperitoneum CT and its application in diagnosis of abdominal adhesion
Authors: Wang Gui-sheng , Zhang Zhi-yi , Qi Xue-ting , Liu Jin , Liu Ting , Zhao Jing-wei , Chen Xiao-xia , Chen Yi 
Published: October 2021

AbstractTo retrospectively analyze the use of artificial pneumoperitoneum in CT scans, to explore its operation methods and technical points, and to lay the foundation for the widespread application of artificial pneumoperitoneum in CT. A total of 331 patients who underwent artificial pneumoperitoneum with CT angiography from January 1, 2013, to November 1, 2019, were recruited. All patients underwent standardized artificial pneumoperitoneum in the horizontal, left and right lateral, and prone positions during CT thin-layer scans of the abdomen and 3D reconstruction. Taking the surgical results as the gold standard, and using kappa test to verify the consistency of surgical results and imaging results. In all 331 patients, 43 patients had a normal peritoneal space, and 288 patients had an abnormal peritoneal space. And only 22 patients developed complications of subcutaneous emphysema, accounting for 6.6% of all 331 patients. In terms of the postoperative results, 28 were normal, and 303 were abnormal. The sensitivity, specificity and accuracy of CT diagnosis of abdominal adhesions using artificial pneumoperitoneum were 100%, 95.04%, and 95.46%, respectively. According to the Kappa consistency test, the imaging diagnosis from the CT scan with artificial pneumoperitoneum had a high consistency with the surgical results (kappa = 0.796, P < 0.05). The technique of artificial pneumoperitoneum CT is safe, reliable, highly practical, and proficient for obtaining good imaging results. It provides a good imaging basis for the diagnosis of intra-abdominal diseases, especially intra-abdominal adhesions.
Expression of p53, p63, podoplanin and Ki-67 in recurring versus non-recurring oral leukoplakia
Authors: Sundberg Jonas , Pandey Sushma , Giglio Daniel , Holmberg Erik , Kjeller Göran , Kovács Anikó , Sand Lars Peter , Tokozlu Burcu , Öhman Jenny , Sapkota Dipak , Hasséus Bengt 
Published: October 2021

AbstractOral leukoplakia (OL), a potentially malignant disorder, recurs in 40% of cases after surgical removal. Recurrence is a risk factor for malignant transformation. We aimed to examine the prognostic significance of four biomarkers related to cell proliferation: p53, p63, podoplanin (PDPN) and Ki-67 in predicting recurrence. Formalin-fixed-paraffin-embedded specimens from excised OL (n = 73, 33 recurrent; 40 non-recurrent) were collected in a prospective study. Immunohistochemistry was used to visualise expression of p53, p63, PDPN and Ki-67. Image analysis software was used for quantification of p53-, p63- and Ki-67-expressing cells, while PDPN was analysed visually. The expression of all four proteins were higher in recurrent compared with non-recurrent OL, only expression of p53 was statistically significant. In uni- and multivariable Cox regression analyses of individual markers, expression of p63 was significantly associated with higher recurrence risk (p = 0.047). OL with a combined high expression of both p53 and p63 had a significantly higher risk to recur [Log Rank,p = 0.036; multivariate Cox, HR: 2.48 (1.13–5.44;p = 0.024)]. Combination of p53 and p63 expression may be used as a prognostic biomarker for recurrence of OL.
Short-term and long-term mate preference in men and women in an Iranian population
Authors: Mirfazeli Fatemeh Sadat , Lai Meng-Chuan , Memari Amirhossein , Rajab Armin , Shafizadeh Milad , Zarei Sahar , Shariat Seyed Vahid , Fashi Maryam Haghighi , Barzegary Ebrahim , Vahabie Abdol-Hossein 
Published: October 2021

AbstractMate preference in short-term relationships and long-term ones may depend on many physical, psychological, and socio-cultural factors. In this study, 178 students (81 females) in sports and 153 engineering students (64 females) answered the systemizing quotient (SQ) and empathizing quotient (EQ) questionnaires and had their digit ratio measured. They rated their preferred mate on 12 black-line drawing body figures varying in body mass index (BMI) and waist to hip ratio (WHR) for short-term and long-term relationships. Men relative to women preferred lower WHR and BMI for mate selection for both short-term and long-term relationships. BMI and WHR preference in men is independent of each other, but has a negative correlation in women. For men, digit ratio was inversely associated with BMI (p = 0.039, B = − 0.154) preference in a short-term relationship, and EQ was inversely associated with WHR preference in a long-term relationship (p = 0.045, B = − 0.164). Furthermore, men and women in sports, compared to engineering students, preferred higher (p = 0.009, B = 0.201) and lower BMI (p = 0.034, B = − 0.182) for short-term relationships, respectively. Women were more consistent in their preferences for short-term and long-term relationships relative to men. Both biological factors and social/experiential factors contribute to mate preferences in men while in women, mostly social/experiential factors contribute to them.
The associations between maternal BMI and gestational weight gain and health outcomes in offspring at age 1 and 7 years
Authors: Chiavaroli Valentina , Hopkins Sarah A. , Biggs Janene B. , Rodrigues Raquel O. , Seneviratne Sumudu N. , Baldi James C. , McCowan Lesley M. E. , Cutfield Wayne S. , Hofman Paul L. , Derraik José G. B. 
Published: October 2021

AbstractIn secondary analyses of a randomised controlled trial of exercise during pregnancy, we examined associations between mid-pregnancy maternal body mass index (BMI) and excessive gestational weight gain (GWG) with offspring health. Follow-up data were available on 57 mother–child pairs at 1-year and 52 pairs at 7-year follow-ups. Clinical assessments included body composition and fasting blood tests. At age 1 year, increased maternal BMI in mid-gestation was associated with greater weight standard deviation scores (SDS) in the offspring (p = 0.035), with no observed associations for excessive GWG. At age 7 years, greater maternal BMI was associated with increased weight SDS (p < 0.001), BMI SDS (p = 0.005), and total body fat percentage (p = 0.037) in their children. Irrespective of maternal BMI, children born to mothers with excessive GWG had greater abdominal adiposity (p = 0.043) and less favourable lipid profile (lower HDL-C and higher triglycerides). At 7 years, maternal BMI and excessive GWG had compounded adverse associations with offspring adiposity. Compared to offspring of mothers with overweight/obesity plus excessive GWG, children of normal-weight mothers with adequate and excessive GWG were 0.97 and 0.64 SDS lighter (p = 0.002 and p = 0.014, respectively), and 0.98 and 0.63 SDS leaner (p = 0.001 and p = 0.014, respectively). Both greater maternal BMI in mid-pregnancy and excessive GWG were independently associated with increased adiposity in offspring at 7 years.