bluuebunny/crossref_metadata_2025_split huggingface dataset:
Downstream of GA4, PbCYP78A6 Regulates Parthenogenesis by Mediating Cell Cycle-Related Genes in Pear (Pyrus Bretshneider Rehd.)
Authors: Zhang Haiqi , Han Wei , Wang Huibin , Cong Liu , Zhai Rui , Yang Chengquan , Wang Zhigang , Xu Lingfei 
Published: April 2021

Abstract
Background: Parthenocarpy results in traits attractive to both consumers and breeders, and it overcomes the obstacle of self-incompatibility in the fruit set of horticultural crops, including pear (Pyrus bretshneider). However, there is limited knowledge regarding the genetic and molecular mechanisms that regulate parthenogenesis. Results: Here, in a transcriptional comparison between pollination-dependent and GA4-induced parthenocarpy, PbCYP78A6 was identified and proposed as a candidate gene involved in parthenocarpy. PbCYP78A6 is similar to Arabidopsis thaliana CYP78A6 and is highly expressed in pear hypanthia. The increased PbCYP78A6 expression, as assessed by RT-qPCR, was induced by pollination and GA4 exposure. The ectopic overexpression of PbCYP78A6 contributed to parthenocarpic fruit production in tomato. The PbCYP78A6 expression coincided with fertilized and parthenocarpic fruitlet development and the expression of fruit development-related genes as assessed by cytological observations and RT-qPCR, respectively. PbCYP78A6 RNA interference and overexpression revealed that the gene is an upstream regulator of fruit development-related genes in pear. Conclusions: Our findings indicate that PbCYP78A6 plays a critical role in cell proliferation and provide insights into controlling parthenocarpy.
Long Noncoding RNA SNHG10 Inhibits Renal Fibrosis By Negatively Regulating MiR-378b Expression
Authors: Hao Jian , Zhou Yun , Yu Weimin , Li Hui , He Dandan 
Published: April 2021

Abstract
Background: LncRNA have been increasingly shown that plays pivotal roles in the development of various diseases, including renal fibrosis. Nevertheless, the pathological function of Long non-coding RNA SNHG10 (SNHG10) in the renal fibrosis remains obscure.Methods: We detected the expression levels of SNHG10 in the tissue samples and cell lines via RT-qPCR analysis. The functions of SNHG10 on the progression of renal fibrosis were examined by CCK-8, EdU, dual luciferase reporter and immunofluorescence analyses.Results: In the present study, SNHG10, production of extracellular matrix (ECM), including α-SMA and Fibronectin levels were significantly increased in HK-2 cells after TGF-β stimulation. Ectopic of SNHG10 inhibited cell proliferation and inhibits theα-SMA and Fibronectin expression of TGF-β1-induced HK-2 cells. In addition, bioinformatics analysis and dual luciferase reporter assay indicated that miR-378b was a target gene of SNHG10. Mechanistically, miR-378b overexpression abolished the repressive effects of SNHG10 on TGF-β1-induced HK-2 cells.Conclusion: SNHG10 plays an anti-fibrotic effect through suppression of miR-378b expression in renal fibrosis, which provides a promising therapeutic target for the treatment of renal fibrosis.
An Ultraviolet Fluorescent Particle Image Velocimetry (PIV) for the Study of Impinging Liquid Film in Centrifuge Accelerator Disk
Authors: Fan Dongdong , Zhu Guorui , Tan Wei 
Published: April 2021

Abstract
In the design of industrial fluid machinery, obstacles structures in fluid channels are frequently encountered, such as the accelerator (or called liquid distributor) in the centrifuge. Traditional Standard Particle Image Velocimetry (PIV) uses a sheet laser to slice a field to form the image plane, requiring strong light transmittance for the measuring objects, which failed in the aspect of poor light conditions. The authors developed a novel PIV which was applicable for the impinging liquid film surrounded by mist and successfully applied it to a semi-enclosed flow field. This PIV system includes newly synthesized fluorescent tracer particles and a set of ultraviolet lights, and the emission intensity satisfied the exposure time of 150μs. Then the PIV was implemented in a full-scale rotating platform to study the flow characteristics of the impinging liquid film in centrifuge accelerator disk. Results show that the velocity of accelerated fluids could be divided into impinging and pushing velocity, and the dominant role of the two velocity components will exchange as the conditions vary. At the flow rate of 12 m3/h, only when the rotating speed was larger than 1650 r/min, the speed-up effect is striking, and at the rotating speed of 2310 r/min, the acceleration effect φ reached 0.52. At the rotating speed of 1650 r/min, the acceleration effect φ reached 0.51 when the flow rate equaled 9 m3/h.
Genome-Scale Metabolic Model Based Engineering of Escherichia Coli Enhances Recombinant Single Chain Antibody Fragment Production
Authors: Behravan Aidin , Hashemi Atieh , Marashi Sayed-Amir , Fouladiha Hamideh 
Published: April 2021

Abstract
Escherichia coli is an attractive and cost-effective cell factory for producing recombinant proteins such as scFvs. AntiEpEX-scFv is a small antibody fragment receiving considerable attention for the epithelial cell adhesion molecule (EpCAM) targeting. EpCAM is one of the first discovered-cancer-associated biomarkers highly expressed on various types of solid tumors. Hereby, a genome-scale metabolic model guided engineering strategy was proposed to recognize gene targets for improved antiEpEX-scFv production. Flux balance analysis and FVSEOF algorithm identified several potential genetic targets localized in the glucose import system and pentose phosphate pathway that probably guaranteed an improved yield of scFv. Among the targets predicted by the model, glk gene encoding glucokinase was selected to be overexpressed in the parent strain Escherichia coli BW25113 (DE3). Due to metabolic burden, scFv recombinant expression caused a remarkable decrease in the maximum specific growth rate of the transformed strain. By means of overexpressing glk, presumably increasing carbon flux through the PP pathway, the growth capacity of the E. coli recombinant strain was recovered. Moreover, the engineered strain with glk overexpression successfully increased scfv production. The titer of antiEpEX-scFv reached 235.41 ± 9.53 µg/mL (0.428 g/g DCW) in the engineered strain compared with the parent strain (110.236 ± 7.68 µg/mL; 0.202 g/g DCW). So, model-based prediction was experimentally validated. This approach can be considered for the improvement of other recombinant proteins production.
Assessment of men involvement in family planning services use and associated factors in rural Upper West Region of Ghana
Authors: Kwawukume Abigail Kpekpo , Laar Alexander Suuk , Abdulai Tanko 
Published: April 2021

Abstract
Background

In low-and-middle come countries (LMICs) less attention is paid to men’ involvement in Family Planning (FP)programs where public health officials have advocated the involvement of men as a strategy for addressing the dismal performance of FP programs. The study assessed factors which promote or hinder the uptake of FP services among partners in a rural setting of northern Ghana.
Methods

A cross-sectional descriptive study design was used to collect data from 200 respondents. Study respondents were selected through random cluster sampling.
Results

The findings showed that men partners’ knowledge (95.5%) and approval (72.8%) of FP services were high. Approval of services was also confirmed by their men partners (75%). Mass media was the commonest source of contraception information (48.1%) with the radio (29.4%) being the most popular source. The findings also indicated that men had a higher propensity (X2 = 4.5534, p = 0.033) of supporting a FP method use. Women who reported that their spouse supported FP method use were more likely to use a contraceptive method (X2 = 9.5223, P = 0.002) if their spouse supported FP method use (X2 = 9.5223, P = 0.002) and if their partners had some education (X2 = 14.1133, P = 0.000). Reasons for low contraceptive use were health risks, side-effects and socio-cultural norms.
Conclusion

Family planning programs need to include men at all levels of health promotion and education of FP programs to help reduce misconceptions about contraceptive
Manufacturing Readiness Assessment for Evaluation of the Microarray Patch Industry: An Exploration of Barriers to Full-Scale Manufacturing
Authors: Creelman Ben , Frivold Collrane , Jessup Sierra , Saxon Gene , Jarrahian Courtney 
Published: April 2021

Abstract
Microarray patch (MAP) technology is a promising new delivery technology for vaccines and pharmaceuticals. Yet due to several differing and novel production methods, barriers to full-scale manufacturing exist. PATH conducted a manufacturing readiness assessment and follow-up interviews to identify both the current manufacturing readiness of the industry as well as how readiness varies by developer type and MAP type. Follow-up interviews identified barriers the industry faces in reaching full manufacturing readiness, including the perceived regulatory and investment risk of manufacturing MAPs at scale due to quality requirements and control methods, uncertain sterility requirements, lack of standard production methods (especially around dissolvable MAP drying methods), and the lack of available contract manufacturing organizations with MAP manufacturing capabilities. A Regulatory Working Group has been established to identify and address critical quality issues specific to MAP manufacturing with the aim of providing developers insight into what will be expected for MAP product approvals. Standardizing MAP production equipment and automatic, visual quality control could reduce the overall risk to developers and contract manufacturing organizations in pursuing pilot-scale manufacturing capabilities and ultimately lower barriers to the scale-up of full medical MAP product lines.
Role of intrauterine balloon stent or oral estrogen on prevention of adhesion after hysteroscopic septectomy in septate uterus: study protocol for a randomized controlled multi-center study in China
Authors: Deng Shan , Zhao Zichen , Feng Limin , Huang Xiaowu , Wang Sumin , Xue Xiang , Yan Lei , Ma Baorong , Hao Lijuan , Li Xueying , Yang Lihua , Song Liwen , Zhang Heping , Chen Zijiang , Zhu Lan 
Published: April 2021

Abstract
• BackgroundThe septate uterus is the most common uterine malformation. The uterus septum increases the possibility of adverse pregnancy outcomes which can be improved by hysteroscopic metroplasty (trans-cervical resection of septate, TCRS). Various methods are used to prevent postoperative intrauterine adhesion in clinical practice. However, there is insufficient evidence to support or oppose the use of any of them. This study will be a multi-center, open labeled, prospective, randomized, controlled study. We will examine whether it is necessary to use the anti-adhesion barrier or a certain dose estrogen to prevent intrauterine adhesion after TCRS and further improve the fertility outcome.• MethodsA total of 243 patients with septate uterus who meet the inclusion criteria will be randomly assigned into three groups with 1:1:1 allocation ratio. Group A will be the control group without any application of anti-adhesion method after TCRS; the participants in group B will be inserted an intrauterine balloon stent after TCRS for one week; the participants in group C will take estradiol valerate (2mg, bid) for 2 months after the surgery. A second-look hysteroscopy will be carried out after two menstrual cycles after the surgery to evaluate the intrauterine condition. Pregnancy outcomes, particularly the live birth rate, will be followed for at least one year.• DiscussionThis study will be the first well-powered clinical trial to assess the effectiveness of the anti-adhesion barrier or a certain dose of estrogen to prevent intrauterine adhesion after TCRS and further improve the fertility outcome. Our findings will guide the clinical care of patients after TCRS.Trial registration: ChiCTR, ChiCTR2000032061. Registered on Apr 19, 2020, http://www.chictr.org.cn/showproj.aspx?proj=52499
A Broadband Dual Circularly Polarized Compact Printed Monopole Antenna
Authors: Saini Rohit Kumar 
Published: April 2021

Abstract
A microstrip line –fed broadband dual circular polarized, two port printed monopole antenna is presented. The antenna consists of a ground plane with arrow shaped stub at the corner and a pair of inverted L-shaped feed lines. The 3dB axial ratio bandwidth of the antenna is about 58%(1.7GHz-3.1GHz) in which the return loss and isolation are better than 10dB and 12dB respectively. A parametric study of proposed antenna’s geometric parameters is given for understanding of the antenna performance. The realize gain, reflection coefficient (S 11 ) and transmission coefficient (S 21 ) are higher than 1, 10 and 12dB respectively within the entire axial ratio bandwidth (ARBW).
Systematic Literature Review of Real-World Evidence of C Eftolozane/Tazobactam for the Treatment of Respiratory Infections
Authors: Puzniak Laura , Dillon Ryan , Palmer Thomas , Collings Hannah , Enstone Ashley 
Published: April 2021

Abstract
Background: Gram-negative nosocomial pneumonia (NP), including hospital-acquired bacterial pneumonia (HABP), ventilated HABP (vHABP), and ventilator-associated bacterial pneumonia (VABP), is a significant cause of morbidity and mortality. Common pathogens, including Enterobacterales and Pseudomonas aeruginosa are highly prevalent in healthcare settings and have few effective treatment options due to high rates of antibacterial resistance. Resistant pathogens are associated with significantly worse outcomes and higher costs, relative to patients with susceptible infections. Ceftolozane/tazobactam (C/T) has established efficacy in clinical trials of patients with NP. This review aimed to collate data on C/T use for HABP/vHABP/VABP infections in real-world clinical practice. Methods: This systematic literature review searched online biomedical databases for real-world studies of C/T for gram-negative respiratory tract infections (RTIs) up to June 2020. Relevant study, patient, and treatment characteristics, microbiology, and efficacy outcomes were captured.Results: Thirty-three studies comprising 658 patients were identified. Pneumonia was the most common infection C/T was used to treat (85%), with a smaller number of unspecified RTIs (9%) and tracheobronchitis (5%) reported. Data on severity of illness and comorbidity were inconsistently reported. The majority of patients had respiratory infections caused by P. aeruginosa (92.8%), of which 88.1% were multidrug-resistant (including extensively drug-resistant or pandrug-resistant). Examination of these studies demonstrated an increase in the percentage of patients receiving the recommended dose of C/T for respiratory infections (3 g q8h or renal impairment-adjusted) over time (36.8% of patients in 2017 to 71.5% in 2020). Clinical success rates ranged from 51.4–100%, with 10 studies (55.6% of studies reporting clinical success) reporting clinical success rates of >70%; microbiological success rates ranged from 57.0–100.0%, with three studies (60.0% of studies reporting microbiological success) reporting microbiological success rates of >70%. Thirty-day mortality ranged from 0.0–33.0%, with nine studies (90% of studies reporting mortality) reporting 30-day mortality of <30%. Conclusions: The studies identified in this review demonstrate that C/T shows similar outcomes as those seen in clinical trials, despite the higher frequency of multidrug-resistant pathogens, and comorbidities/conditions that may have been excluded from the trials.
SARS-CoV-2 B.1.1.7 variant of concern detected in a pet dog and cat after exposure to a person with COVID-19, USA
Authors: Hamer Sarah , Ghai Ria , Zecca Italo B. , Auckland Lisa D. , Roundy Christopher M. , Davila Edward , Busselman Rachel E. , Tang Wendy , Pauvolid-Correa Alex , Killian Mary Lea , Jenkins-Moore Melinda , Torchetti Mia Kim , Austerman Suelee Robbe , Lim Ailam , Akpalu Yao , Fischer Rebecca S. B. , Behravesh Casey Barton , Hamer Gabriel L. 
Published: April 2021

Abstract

As part of a longitudinal household transmission study of pets living with persons with COVID-19 in Texas, two pets were confirmed to be infected with the SARS-CoV-2 B.1.1.7 variant of concern (VOC). The pets were a dog and a cat from the same household, sampled two days after their owner tested positive for COVID-19. The oral, nasal, and fur swabs for both pets tested positive for SARS-CoV-2 by qRT-PCR and consensus whole genome sequences from the dog and cat were 100 % identical and matched the B.1.1.7 VOC. Virus was isolated from the cat’s nasal swab. One month after initial detection of infection, the pets were re-tested twice at which time only the fur swabs (both pets) and oral swab (dog only) remained positive, and neutralizing antibodies for SARS-CoV-2 were present in both animals. Sneezing by both pets was noted by the owner in the weeks between initial and follow-up testing. This study documents the first detection of B.1.1.7. in companion animals in the United States, and the first genome recovery and isolation of B.1.1.7 variant of concern globally in any animal.
Effective health service planning and delivery: A qualitative case study exploring health service users perspectives
Authors: Marme Gigil 
Published: April 2021

Abstract
Introduction: Healthcare service is an essential determinant to population health. This qualitative case study aims to explore health service users’ perspective of effective health services delivery and the current challenges affecting the management and delivery of health services at a primary healthcare facility in Madang Province, Papua New Guinea (PNG). Methods: Qualitative data were collected using semi-structured interviews with key informants (KI) representing academics, undergraduate students, administration staff, patients and healthcare workers. The interviews covered three main areas: users’ views of effective health services, current challenges affecting effective planning and management of primary healthcare services, and interventions to improve health services planning and delivery. Results: The services users associated effective health services delivery with increased availability, accessibility, acceptability, and quality healthcare. Many factors exist to influence the effective planning and delivery of health services. The results show that health systems and personal factors have a major influence on the planning and health services delivery. Conclusion: The findings from this study call for an evaluation of the current healthcare system, particularly at the primary healthcare level, as the primary point of contact to the formal healthcare system, and the need for developing a contextual model of healthcare that meet the needs of the service users. We concluded that if health services users’ perspectives are considered in health policy, the local community may experience significant improvement in health status.
Comparison of Outcomes and Side Effects for Neoadjuvant Chemotherapy with Weekly Cisplatin and Paclitaxel Followed by Chemoradiation versus Chemoradiation Alone in Stage IIB-IVA Cervical Cancer: study protocol for a randomized controlled trial
Authors: Li Jing , Cai Rong , Liu Hua , Shen Lifei , Long Wenqing , Yang Chenmin , Li Ya , Xu Haoping , Xi Wenqi , Feng Weiwei 
Published: April 2021

Abstract
Background

Currently, the standard treatment for locally advanced cervical cancer is concurrent chemoradiation (CCRT). The effect of neoadjuvant chemotherapy in advanced cervical cancer is controversial. Studies have shown that the addition of a weekly regimen of neoadjuvant chemotherapy (NACT) followed by CCRT may be superior to a triweekly regimen of NACT and CCRT. Among patients who had not received prior cisplatin, a cisplatin and paclitaxel (TP) regimen resulted in longer overall survival than other regimens. This study aims to investigate the feasibility, safety and efficacy of NACT with weekly TP followed by CCRT.
Methods

This is a prospective, randomized, open-labeled, multicentered Phase III study with a sample size of 300. Patients with IIB-IVA cervical cancer will be randomly allocated in a 1:1 ratio to one of two intervention arms. Study arm: patients will receive dose-dense cisplatin (40 mg/m2) and paclitaxel (60 mg/m2) weekly for 4 cycles followed by CCRT (45 Gy in 5 weeks concurrent with cisplatin 40 mg/m2 weekly) plus image-guided adaptive brachytherapy (IGBRT). Control arm: patients will undergo CCRT treatment. The primary endpoints of the study are overall survival rate and disease-free response rate; the secondary endpoint is the response rate 3 months after treatment completion; the tertiary endpoints are grade 3–4 adverse effects and quality of life; and potential biomarkers for predicting treatment response will also be studied.
Conclusions

The data gathered from the study will be used to determine whether NACT with weekly TP followed by CCRT may become an optimized treatment for locally advanced cervical cancer.
Trial Registration

Chinese clinical trial registry, ChiCTR1900025327; http://www.chictr.org.cn. Registered 24 August 2019 - Retrospectively registered, medresman.org.cn/ChiCTR1900025326
MHD Flow of a Newtonian Fluid in Symmetric Channel with ABC
Fractional Model Containing Hybrid Nanoparticles
Authors: Chu Yu Ming , Ikram Muhammad Danish , Imran Muhammad Asjad , Akgül Ali 
Published: April 2021

Introduction:
The nanofluid is the novelty of nanotechnology to overcome the
difficulties of heat transfer in several manufacturing and engineering areas. Fractional calculus has
many applications in nearly all fields of science and engineering, which include electrochemistry,
dispersion and viscoelasticity.

Objectives:
This paper focused on the heat transfer of a hybrid nanofluid in two vertical parallel
plates and presented a comparison between fractional operators.

Methods:
In this paper, the fractional viscous fluid model is considered along with physical initial
and boundary conditions for the movement occurrences.
The analytical solutions have been obtained via the Laplace transform method for the
concentration, temperature and velocity fields. After that, we have presented a comparison
between Atangana-Baleanu (ABC), Caputo (C) and Caputo-Fabrizio (CF) fractional operators.

Results:
The comparison of different base fluids (Water, kerosene, Engine Oil) is discussed
graphically with respect to temperature and velocity.
The results show that due to the high thermal conductivity of water, temperature and velocity are
high. While engine oil has maximum viscosity than water and kerosene, thus temperature and
velocity are very low. However, due to the improvement in the thermal conductivity with the
enrichment of hybrid nanoparticles, the temperature increased, and since the viscosity also
increased, the velocity got reduced.

Conclusion:
Atangana-Baleanu (ABC) fractional operator provided better memory effect of
concentration, temperature and velocity fields than Caputo (C) and Caputo-Fabrizio (CF).
Temperature and velocity of water with hybridized nanoparticles were high in comparison to
kerosene and engine oil.