bluuebunny/crossref_metadata_2025_split huggingface dataset:
Resting State Networks Related to the Maintenance of Good Cognitive Performance During Healthy Aging
Authors: Maesawa Satoshi , Mizuno Satomi , Bagarinao Epifanio , Watanabe Hirohisa , Kawabata Kazuya , Hara Kazuhiro , Ohdake Reiko , Ogura Aya , Mori Daisuke , Nakatsubo Daisuke , Isoda Haruo , Hoshiyama Minoru , Katsuno Masahisa , Saito Ryuta , Ozaki Norio , Sobue Gen 
Published: November 2021

Purpose: Maintenance of cognitive performance is important for healthy aging. This study aims to elucidate the relationship between brain networks and cognitive function in subjects maintaining relatively good cognitive performance.Methods: A total of 120 subjects, with equal number of participants from each age group between 20 and 70 years, were included in this study. Only participants with Addenbrooke’s Cognitive Examination – Revised (ACE-R) total score greater than 83 were included. Anatomical T1-weighted MR images and resting-state functional MR images (rsfMRIs) were taken from all participants using a 3-tesla MRI scanner. After preprocessing, several factors associated with age including the ACE-R total score, scores of five domains, sub-scores of ACE-R, and brain volumes were tested. Morphometric changes associated with age were analyzed using voxel based morphometry (VBM) and changes in resting state networks (RSNs) were examined using dual regression analysis.Results: Significant negative correlations with age were seen in the total gray matter volume (GMV, r = −0.58), and in the memory, attention, and visuospatial domains. Among the different sub-scores, the score of the delayed recall (DR) showed the highest negative correlation with age (r = −0.55, p &lt; 0.001). In VBM analysis, widespread regions demonstrated negative correlation with age, but none with any of the cognitive scores. Quadratic approximations of cognitive scores as functions of age showed relatively delayed decline compared to total GMV loss. In dual regression analysis, some cognitive networks, including the dorsal default mode network, the lateral dorsal attention network, the right / left executive control network, the posterior salience network, and the language network, did not demonstrate negative correlation with age. Some regions in the sensorimotor networks showed positive correlation with the DR, memory, and fluency scores.Conclusion: Some domains of the cognitive test did not correlate with age, and even the highly correlated sub-scores such as the DR score, showed delayed decline compared to the loss of total GMV. Some RSNs, especially involving cognitive control regions, were relatively maintained with age. Furthermore, the scores of memory, fluency, and the DR were correlated with the within-network functional connectivity values of the sensorimotor network, which supported the importance of exercise for maintenance of cognition.
Floating-Harbor Syndrome Treated With Recombinant Human Growth Hormone: A Case Report and Literature Review
Authors: Bo Hui , Jiang Lihong , Zheng Jiaqi , Sun Jie 
Published: November 2021

Introduction: We aimed to summarize the clinical characteristics of Floating-Harbor syndrome (FHS) and the effect of recombinant human growth hormone (rhGH) to increase height.Methods: The clinical manifestations, gene sequencing results, treatment, and regression of one child with FHS were reported at the Department of Pediatrics, General Hospital of Tianjin Medical University, in July 2020. PubMed was searched using the keyword “Floating-Harbor Syndrome” up to March 2021 to obtain clinical information on children with FHS for review.Results: The child, who was a male aged 6 years and 9 months, presented to the clinic with main complaints of delayed language development since childhood and a short stature for 4 years. The child's short stature, peculiar facial features, delayed language development, and delayed bone development were considered alongside genetic testing and Sanger sequencing to verify the results. A heterozygous mutation (c.7401delC; p.Ile2468Phefs*7) was identified in exon 34 of the SRCAP gene, which was a frameshift mutation, and Sanger verification showed that neither parent had this mutation. The child was administered subcutaneous injection of rhGH (0.13 U/kg/day) and was followed up regularly. At the time of writing, the child had been treated for 6 months and was 7 years and 3 months old with a height of 106.3 cm (−3.69 SDS), which was a height increase of 6.3 cm. The patient did not complain of discomfort during treatment and presented normal laboratory tests results. Twenty-two children with FHS treated with rhGH were included in the literature review, and most of these patients demonstrated an increase in height SDS without adverse effects.Conclusion: Short stature, delayed skeletal maturation, impaired language expression, intellectual deficits, and peculiar facial features are the main clinical features of FHS. rhGH can be used as a treatment to increase height in patients with FHS, but its effectiveness and safety still need to be monitored in larger sample sizes over longer periods of time.
Monitoring Tacrolimus Concentrations in Whole Blood and Peripheral Blood Mononuclear Cells: Inter- and Intra-Patient Variability in a Cohort of Pediatric Patients
Authors: De Nicolò Amedeo , Pinon Michele , Palermiti Alice , Nonnato Antonello , Manca Alessandra , Mula Jacopo , Catalano Silvia , Tandoi Francesco , Romagnoli Renato , D’Avolio Antonio , Calvo Pier Luigi 
Published: November 2021

Tacrolimus (TAC) is a first-choice immunosuppressant for solid organ transplantation, characterized by high potential for drug-drug interactions, significant inter- and intra-patient variability, and narrow therapeutic index. Therapeutic drug monitoring (TDM) of TAC concentrations in whole blood (WB) is capable of reducing the incidence of adverse events. Since TAC acts within lymphocytes, its monitoring in peripheral blood mononuclear cells (PBMC) may represent a valid future alternative for TDM. Nevertheless, TAC intracellular concentrations and their variability are poorly described, particularly in the pediatric context. Therefore, our aim was describing TAC concentrations in WB and PBMC and their variability in a cohort of pediatric patients undergoing constant immunosuppressive maintenance therapy, after liver transplantation. TAC intra-PBMCs quantification was performed through a validated UHPLC–MS/MS assay over a period of 2–3 months. There were 27 patients included in this study. No significant TAC changes in intracellular concentrations were observed (p = 0.710), with a median percent change of −0.1% (IQR −22.4%–+46.9%) between timings: this intra-individual variability was similar to the one in WB, −2.9% (IQR −29.4–+42.1; p = 0.902). Among different patients, TAC weight-adjusted dose and age appeared to be significant predictors of TAC concentrations in WB and PBMC. Intra-individual seasonal variation of TAC concentrations in WB, but not in PBMC, have been observed. These data show that the intra-individual variability in TAC intracellular exposure is comparable to the one observed in WB. This opens the way for further studies aiming at the identification of therapeutic ranges for TAC intra-PBMC concentrations.
Age-Related Differences in Motivation of Recreational Runners, Marathoners, and Ultra-Marathoners
Authors: Gerasimuk Dagmara , Malchrowicz-Mośko Ewa , Stanula Arkadiusz , Bezuglov Eduard , Achkasov Evgenij , Swinarew Andrzej , Waśkiewicz Zbigniew 
Published: November 2021

Aim: This study was aimed to investigate the influence of age on the motivations of various types of runners, namely, marathoners, ultra-marathoners, and non-starters.Methods: A total of 1,537 runners including 380 women (24.7%) and 1,157 men (75.3%) took part in the diagnostic survey and completed the motivations of marathoners’ scales questionnaire (MOMS). The effect sizes were estimated.Results: The article presents several statistically significant differences in the impact of age on the motivations of runners in different categories and compares the motivations of marathon runners, ultramarathon runners, and non-starters. The results show that young non-starters decide to run for personal goal achievement, and for both marathon and ultramarathon runners, recognition and competition are important. However, for older people in all groups (non-starters, marathoners, and ultramarathoners), personal goal achievement is of the least importance. Among the oldest runners, the most important motives were self-esteem for non-starters and health orientation for marathoners and ultramarathoners.
Unique Trans-fatty Acid Profile in Children With Attention Deficit Hyperactivity Disorder
Authors: Armon-Omer Ayelet , Amir Eti , Neuman Hadar , Khateeb Saleh , Mizrachi Itai , Shalan Monia , Tamir Snait , Yatzkar Uri 
Published: November 2021

Background: Attention deficit hyperactivity disorder (ADHD) is the most common developmental disorder in children. Studies suggest an association between fatty acids composition and ADHD pathogenesis. We aimed to investigate whether children diagnosed with ADHD present unique fatty acid profiles in red blood cells (RBC), as compared to children without ADHD.Method: We examined 60 children aged 6–14 years, out of which 32 were diagnosed with ADHD, and 28 were not. Blood was collected from all children to quantify an array of 26 fatty acids from RBC membranes. Fatty acid methyl esters were generated by acid transesterification and analyzed by gas chromatography.Results: We found that children with ADHD presented unique fatty acid profiles on RBC membranes with significantly higher levels of most of the trans-fatty acids (Total trans-fatty acids 0.64 ± 0.21 vs. 0.49 ± 0.18 p = 0.003) and lower levels of docosahexaenoic acid (DHA), as compared to controls (4.06 ± 0.79 vs. 4.68 ± 1.37 p = 0.040). Additionally, total trans-fatty acids were higher in children with extremely severe clinical ADHD condition score, as compared to milder ADHD scores and to control children (0.72 ± 0.18, 0.64 ± 0.20, 0.61 ± 0.22, 0.49 ± 0.18, p = 0.010, accordingly).Conclusion: Children with ADHD have higher levels of trans-fatty acids in RBCs, compared to children without ADHD. This study points to a possible link between trans-fatty acids and ADHD. Understanding these findings and the clinical meaning will potentially contribute to a more targeted dietary intervention.
Is the Scope of Costs Considered in Budget Impact Analyses for Anticancer Drugs Rational? A Systematic Review and Comparative Study
Authors: Ma Yue , Li Yuxin , Ma Aixia , Li Hongchao 
Published: November 2021

Background: With the increasing disease burden of cancer worldwide, more and more anticancer drugs have been approved in many countries, and the results of budget impact analyses (BIAs) have become important evidence for related reimbursement decisions.Objectives: We systematically reviewed whether BIAs for anticancer drugs consider the scope of costs rationally and compared the results of different cost scopes to provide suggestions for future analyses and decision-making.Methods: Eligible BIAs published in PubMed, Embase, Web of Science, and the Cochrane Library from 2016 to 2021 were identified based on Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines. We extracted 15 terms from the included studies and analyzed how they considered the scope of costs. In addition, a budget impact model was developed for the introduction of geptanolimab to China's National Reimbursement Drug List to enable a comparison of two cost-scope scenarios.Results: A total of 29 studies were included in the systematic review. All 29 studies considered the costs of anticancer drugs, and 25 (86%) also considered condition-related costs, but only 11 (38%) considered subsequent treatment costs. In the comparative study, the predicted budget impacts from 2022 to 2024 were significantly impacted by subsequent treatment costs, with annual differences between the two cost-scope scenarios of $39,546,664, $65,866,161, and $86,577,386, respectively.Conclusions: The scope of costs considered in some existing BIAs for anticancer drugs are not rational. The variations between different cost scopes in terms of budget impact were significant. Thus, BIAs for anticancer drugs should consider a rational scope of costs that adheres to BIA guidelines. Researchers and decision-makers should pay more attention to the scope of costs to achieve better-quality BIAs for anticancer drugs and enhance reimbursement decision-making.
Molecular Detection of Echinococcus granulosus Sensu Stricto in Environmental Dog Faecal Samples from the Magallanes Region, Patagonia, Chile
Authors: Alvarez Juan Francisco , Ruiz Raúl , Ríos Juan , Alvarez Rojas Cristian A. 
Published: November 2021

Echinococcus granulosus is endemic in the Magallanes region and other areas of Chile. After a successful control programme implemented between 1979 and 2004, dogs’ prevalence decreased from 70% to 0.5%. Since the end of the programme, no prevalence study of canine echinococcosis has been performed in this region. Dog faecal samples were collected from epidemiological units for DNA isolation and polymerase chain reaction (PCR) to detect E. granulosus. In addition, dog owners were required to answer a questionnaire for a risk factor analysis. We collected 1069 environmental dog faecal samples from 267 urban, 241 periurban, and 61 rural epidemiological units. E. granulosus was found in 11/61 (18%) and 1/241 (0.4%) epidemiological units from rural and periurban areas, respectively. The Ultima Esperanza province showed the highest prevalence with eight out of 16 units with faeces positive to E. granulosus showing a main spatial cluster of canine echinococcosis. None of the risk factors investigated showed a statistical significance with positive units. This study shows the active transmission of the parasite in the Magallanes region, especially in the Ultima Esperanza province, with a possible re-emergence of the parasite. Further studies focusing on the incidence in humans are required together with the reestablishment of the compulsory deworming of dogs.
Aplikasi Karbon Ampas Teh Tersulfonasi Sebagai Katalis Dalam Produksi Biodiesel Dari Pfad (Palm Fatty Acid Destilate)
Authors: Ghani Isva Abdul , Ikhsan Muhammad Hiknul , Nizar Umar Kalmar , Dewata Indang , Amran Ali , Sury Suryelita , Sanjaya Hary 
Published: November 2021

Pada penelitian ini digunakan katalis asam padat berbasis karbon ampas teh tersulfonasi dalam proses produksi biodiesel. Penelitian ini bertujuan untuk mensintesis katalis asam padat, mengkarakterisasi sifat fisikokimia katalis, dan menguji aktivitas katalitik katalis dalam produksi biodiesel. Katalis disintesis melalui proses karbonisasi dengan aliran gas N2 dan tanpa aliran gas N2 pada suhu 350˚C selama 1jam. Kemudian dilanjutkan dengan proses sulfonasi mengunakan H2SO4 selama 4jam. Katalis yang dihasilkan dikarakterisasi mengunakan FTIR dan penentuan jumlah situs asam katalis. Data FTIR sampel katalis dengan aliran gas N2 tersulfonasi (SCCS-N2) dan sampel karbon tanpa aliran gas N2 tersulfonasi (SCCS-TN2) menunjukkan puncak transmittan yang kuat yang diamati pada bilangan gelombang 1190 cm-1 dan 1060cm-1 yang mengindikasi adanya vibrasi simetris dan asimetris dari gugus O=S=O. Pada penentuan jumlah situs asam katalis didapatkan jumlah situs asam pada katalis SCCS-N2 yaitu 700 µmol/g dan jumlah situs asam katalis SCCS-TN2 yaitu 600 µmol/g. Pada uji aktivitas katalitik katalis didapatkan densitas biodiesel yang dihasilkan yaitu 0,8434-0,8714 g/ml dan persen konversi tertinggi terdapat pada biodiesel dari katalis SCCS-N2 yaitu 25,4523%.
Excretion Patterns of Urinary Sediment and Supernatant Podocyte Biomarkers in Patients with CKD
Authors: Fukuda Akihiro , Minakawa Akihiro , Sato Yuji , Shibata Hirotaka , Hara Masanori , Fujimoto Shouichi 
Published: November 2021

Background
Podocyte depletion causes glomerulosclerosis, and persistent podocyte loss drives progression to ESKD. Urinary sediment podocin (u-sed Pod) mRNA excretion and urinary supernatant podocalyxin (u-sup PCX) protein have been used to monitor disease activity in glomerular diseases. However, the differences in these markers among pathologies have not been investigated. We examined the roles of these markers in kidney diseases.

Methods
From January 2013 to March 2016, early morning urine samples were collected from 12 healthy controls and 172 patients with kidney disease (n=15 patients with minor glomerular abnormality with mild proteinuria and/or microscopic hematuria, n=15 with minimal change nephrotic syndrome [MCNS], n=15 with membranous nephropathy [MN], n=60 with IgA nephropathy [IgAN], n=19 with crescentic GN [Cres GN], n=10 with lupus nephritis [LN], and n=38 with other kidney diseases). We examined u-sed Pod mRNA excretion, u-sup PCX protein, and the urinary protein-creatinine ratio (u-PCR).

Results
u-sed Pod mRNA excretion was significantly correlated with u-sup PCX protein (r=0.37, P<0.001). Both u-sed Pod mRNA excretion and u-sup PCX protein were significantly correlated with u-PCR (r=0.53, P<0.001 and r=0.35, P<0.001, respectively). Interestingly, u-sed Pod mRNA excretion was significantly increased in proliferative-type GN—including IgAN with extracapillary proliferative lesions, Cres GN, and LN class IV—and significantly correlated with the rate of crescent formation, whereas u-sup PCX protein was significantly increased only in those with MN and subepithelial dense deposit–type LN compared with controls.

Conclusions
Higher u-sed Pod mRNA excretion and u-sup PCX protein were associated with proliferative-type GN, indicating podocyte detachment and subepithelial dense deposit–type GN, respectively. The results suggest that u-sed Pod mRNA excretion and u-sup PCX protein have usefulness for the diagnosis and measurement of disease activity with regard to glomerular diseases.
Single-Cell RNA Sequencing of Urinary Cells Reveals Distinct Cellular Diversity in COVID-19–Associated AKI
Authors: Cheung Matthew D. , Erman Elise N. , Liu Shanrun , Erdmann Nathaniel B. , Ghajar-Rahimi Gelare , Moore Kyle H. , Edberg Jeffrey C. , George James F. , Agarwal Anupam 
Published: November 2021

Background
AKI is a common sequela of infection with SARS-CoV-2 and contributes to the severity and mortality from COVID-19. Here, we tested the hypothesis that kidney alterations induced by COVID-19–associated AKI could be detected in cells collected from urine.

Methods
We performed single-cell RNA sequencing (scRNAseq) on cells recovered from the urine of eight hospitalized patients with COVID-19 with (n=5) or without AKI (n=3) as well as four patients with non–COVID-19 AKI (n=4) to assess differences in cellular composition and gene expression during AKI.

Results
Analysis of 30,076 cells revealed a diverse array of cell types, most of which were kidney, urothelial, and immune cells. Pathway analysis of tubular cells from patients with AKI showed enrichment of transcripts associated with damage-related pathways compared with those without AKI. ACE2 and TMPRSS2 expression was highest in urothelial cells among cell types recovered. Notably, in one patient, we detected SARS-CoV-2 viral RNA in urothelial cells. These same cells were enriched for transcripts associated with antiviral and anti-inflammatory pathways.

Conclusions
We successfully performed scRNAseq on urinary sediment from hospitalized patients with COVID-19 to noninvasively study cellular alterations associated with AKI and established a dataset that includes both injured and uninjured kidney cells. Additionally, we provide preliminary evidence of direct infection of urinary bladder cells by SARS-CoV-2. The urinary sediment contains a wealth of information and is a useful resource for studying the pathophysiology and cellular alterations that occur in kidney diseases.
Pengembangan Media Carier Box dalam Bimbingan Kelompok untuk Pengenalan Karir di PAUD
Authors: Oktaviana Mimbar , Sriyanti Sriyanti 
Published: November 2021

Penggunaan media pembelajaran pada proses belajar mengajar pada jenjang PAUD sangat membantu untuk meningkatkan pemahaman siswa. Penelitian ini didasarkan pada kurang bervariasinya media pembelajaran yang digunakan pada jenjang PAUD khususnya dalam pengenalan karir. Penggunaan media yang slama ini digunakan hanya memfungsikan indra penglihatan dan pendengaran siswa sehingga membuat penyampaian materi ke siswa hanya sebesar 10%. Berdasarkan hal tersebut maka perlu adanya media yang bisa meningkatkan aktifitas pembelajaran dengan memberikan siswa pengalaman tiruan. Media tersebut adalah media carier box yang dapat dilaksanakan dalam bimbinan kelompok. Tujuan dari peneitian ini adalah membuat media pembelajaran yang menyenangkan dan dapat memaksimalkan penyampaian materi kepada siswa, sehingga siswa bisa lebih mengenal tentang macam-macam jenis pekerjaan. Penelitian ini menggunakan model pengembangan Borg & Gall dengan 4 tahap yaitu analisis produk, pengembangan produk awal, validasi ahli dan revisi, uji coba lapangan skala kecil dan revisi produk Hasil penelitian didapat dari hasil uji validitas dari ahli materi, ahli media, ahli praktisi yang kemudian diolah menjadi persentase. Dari hasil uji coba ahli dengan ahli materi diperoleh rerata persentase sebesar 89,2%, hasil ahli media mencapai 81,5%. Kemudian juga dari hasil uji coba skala kecil dengan meminta menilaian dua orang praktisi diperoleh rerata sebesar 89,12% dan 88,55%. Selanjutnya rata rata dari semua presentase hasil validasi adalah 87,09%. Setelah mendapatkan data tersebut dihasilkan pula data kualitatif bahwa media carier box termasuk dalam kategori sangat baik dan sesuai dengan kriteria kelayakan sehingga dapat digunakan sebagai salah satu media pembelajaran dalam bimbingan kelompok di jenjang PAUD
MENGEMBANGKAN SDM UNGGUL DI DESA SIRNABAYA KECAMATAN TELUK JAMBE TIMUR
Authors: Arif Rahman Hakim  , Giri Wahyu Putra  
Published: November 2021

Sebagai daerah yang berubah fungsi menjadi daerah industri, Kabupaten Karawang memiliki slogan baru, yaitu Karawang sebagai kota industri. Tantangan bagi penduduk Karawang adalah kesiapan memasuki dunia industri, keterampilan sebagai petani bagi penduduk Karawang sudah tidak diragukan lagi, akan tetapi memasuki dunia industri, maka pengetahuan, keterampilan serta sikap yang dimiliki perlu dilatih dan dikembangkan agar siap menghadapi era industri. Pengabdian ini bertujuan untuk mendapatkan gambaran terkait sumber daya manusia di desa Sirnabaya. Instrumen yang digunakan dalam pengabdian ini adalah dengan menggunakan kuesioner profil desa berkelanjutan. Teknik pengumpulan data dengan melakukan interviu dengan pihak perwakilan desa yang ditunjuk oleh kepala desa Sirnabaya. Sumber data diklasifikasikan menjadi dua yaitu data primer dan data sekunder. Data primer diperoleh melalui kuesioner, sedangkan data sekunder diperoleh melalui laporan dari Badan Pusat Statistik Nasional. Data hasil kuesioner tersebut dijadikan sebagai dasar pembuatan rancangan program untuk pengembangan SDM unggul di Desa Sirnabaya.
Kata kunci: Sumber daya manusia, pelatihan dan pengembangan
As an area that has changed its function to an industrial area, Karawang Regency has a new slogan, namely Karawang as an industrial city. The challenge for Karawang residents is a readiness to enter the industrial world, skills as farmers for Karawang residents are no longer in doubt, but entering the world industry, their knowledge, skills, and attitudes are necessary and developed to be ready to face the industry. This service aims to get an overview of human resources in Sirnabaya village. The instrument used in this service was to use a sustainable village profile questionnaire. The data technique was conducted by conducting interviews with village representatives appointed by the village head of Sirnabaya. There are two sources of classification data, namely primary data and secondary data. Primary data were obtained through questionnaires, while data were obtained through reports from the National Statistics Agency. The data from the questionnaire was used as the basis for making a program design for the development of superior human resources in Sirnabaya Village.
Keywords: Human resources, training, and development