bluuebunny/crossref_metadata_2025_split huggingface dataset:
Circadian misalignment by environmental light/dark shifting causes circadian disruption in colon
Authors: Tran Laura , Jochum Sarah B. , Shaikh Maliha , Wilber Sherry , Zhang Lijuan , Hayden Dana M. , Forsyth Christopher B. , Voigt Robin M. , Bishehsari Faraz , Keshavarzian Ali , Swanson Garth R. 
Published: June 2021

Background
Physiological circadian rhythms (CRs) are complex processes with 24-hour oscillations that regulate diverse biological functions. Chronic weekly light/dark (LD) shifting (CR disruption; CRD) in mice results in colonic hyperpermeability. However, the mechanisms behind this phenomenon are incompletely understood. One potential innovative in vitro method to study colonic CRs are colon organoids. The goals of this study were to utilize circadian clock gene Per2 luciferase reporter (Per2::Luc) mice to measure the effects of chronic LD shifting on colonic tissue circadian rhythmicity ex vivo and to determine if organoids made from shifted mice colons recapitulate the in vivo phenotype.

Methods
Non-shifted (NS) and shifted (S) BL6 Per2::Luc mice were compared after a 22-week experiment. NS mice had a standard 12h light/12h dark LD cycle throughout. S mice alternated 12h LD patterns weekly, with light from 6am-6pm one week followed by shifting light to 6pm-6am the next week for 22 weeks. Mice were tested for intestinal permeability while colon tissue and organoids were examined for CRs of bioluminescence and proteins of barrier function and cell fate.

Results
There was no absolute difference in NS vs. S 24h circadian period or phase. However, chronic LD shifting caused Per2::Luc S mice colon tissue to exhibit significantly greater variability in both the period and phase of Per2::Luc rhythms than NS mice colon tissue and organoids. Chronic LD shifting also resulted in increased colonic permeability of the Per2::Luc mice as well as decreased protein markers of intestinal permeability in colonic tissue and organoids from shifted Per2:Luc mice.

Conclusions
Our studies support a model in which chronic central circadian disruption by LD shifting alters the circadian phenotype of the colon tissue and results in colon leakiness and loss of colonic barrier function. These CRD-related changes are stably expressed in colon stem cell derived organoids from CRD mice.
Evidence on access to healthcare information by women of reproductive age in low- and middle-income countries: Scoping review
Authors: Shatilwe Joyce Twahafifwa , Kuupiel Desmond , Mashamba-Thompson Tivani P. 
Published: June 2021

BackgroundA majority of women of reproductive age in low- and middle-income countries (LMICs) are not able to access healthcare information due to different factors. This scoping review aimed to map the literature on access to healthcare information by women of reproductive age in LMICs.MethodsThe literature search was conducted through the following databases: Google Scholar, Science Direct, PubMed, EBSCOhost (Academic search complete, CINAHL with full text, MEDLINE with full text, MEDLINE, and PsycINFO), Emerald, Embase, published and peer-reviewed journals, organizational projects, reference lists, and grey literature.ResultsA total of 377 457 articles were identified from all the databases searched. Of these, four articles met inclusion criteria after full article screening and were included for data extraction. The themes that emerged from our study are as follows: accessibility, financial accessibility/affordability, connectivity, and challenges. This study demonstrated that there are minimal interventions that enable women of reproductive age to access healthcare information in terms of accessibility, financial accessibility, and connectivity.ConclusionThe findings of the study revealed poor access and utilization of healthcare information by women of reproductive age. We, therefore, recommend primary studies in other LMICs to determine the accessibility, financial accessibility, connectivity, and challenges faced by women of reproductive age in LMICs.
Effectiveness of attentional bias modification training as add-on to regular treatment in alcohol and cannabis use disorder: A multicenter randomized control trial
Authors: Heitmann Janika , van Hemel-Ruiter Madelon E. , Huisman Mark , Ostafin Brian D. , Wiers Reinout W. , MacLeod Colin , DeFuentes-Merillas Laura , Fledderus Martine , Markus Wiebren , de Jong Peter J. 
Published: June 2021

Background
Attentional bias for substance-relevant cues has been found to contribute to the persistence of addiction. Attentional bias modification (ABM) interventions might, therefore, increase positive treatment outcome and reduce relapse rates. The current study investigated the effectiveness of a newly developed home-delivered, multi-session, internet-based ABM intervention, the Bouncing Image Training Task (BITT), as an add-on to treatment as usual (TAU).

Methods
Participants (N = 169), diagnosed with alcohol or cannabis use disorder, were randomly assigned to one of two conditions: the experimental ABM group (50%; TAU+ABM); or the control group (50%; split in two subgroups the TAU+placebo group and TAU-only group, 25% each). Participants completed baseline, post-test, and 6 and 12 months follow-up measures of substance use and craving allowing to assess long-term treatment success and relapse rates. In addition, attentional bias (both engagement and disengagement), as well as secondary physical and psychological complaints (depression, anxiety, and stress) were assessed.

Results
No significant differences were found between conditions with regard to substance use, craving, relapse rates, attentional bias, or physical and psychological complaints.

Conclusions
The findings may reflect unsuccessful modification of attentional bias, the BITT not targeting the relevant process (engagement vs. disengagement bias), or may relate to the diverse treatment goals of the current sample (i.e., moderation or abstinence). The current findings provide no support for the efficacy of this ABM approach as an add-on to TAU in alcohol or cannabis use disorder. Future studies need to delineate the role of engagement and disengagement bias in the persistence of addiction, and the role of treatment goal in the effectiveness of ABM interventions.
Associations of nerve conduction parameters and OCT angiography results in adolescents with type 1 diabetes
Authors: Wysocka-Mincewicz Marta , Gołębiewska Joanna , Baszyńska-Wilk Marta , Olechowski Andrzej , Byczyńska Aleksandra , Mazur Maria , Nowacka-Gotowiec Monika 
Published: June 2021

Aim
To evaluate dependence of abnormalities in peripheral nerves and retina in children with type 1 diabetes (T1D) using optical coherence tomography angiography (OCTA) and nerve conduction studies (NCS).

Material and methods
50 adolescents with T1D without any signs and symptoms of diabetic retinopathy and neuropathy (mean age 16.92±1.6 years, diabetes duration 6.88 ±4.34years) were included. In OCTA capillary plexuses superficial (SCP) and deep (DCP) vessel density: whole, foveal and parafoveal, ganglion cell complex (GCC), loss volume focal (FLV) and global loss volume (GLV) were analyzed in relation to NCS parameters (motor nerves median and tibial potential amplitude (CMAP), velocity (CV), distal latency (DML) and F wave and sensory nerves median and sural potential amplitude (SNAP), CV and distal latency (DSL).

Results
We detected the correlations between median sensory SNAP and GCC (r = -0.3, p <0.04), motor nerves tibial DML and CV and FLV (respectively r = -0.53, p<0.001, and r = -0.34, p<0.05), and median DML and GLV (r = 0.47, p<0.001). Vessel densities were related to changes in motor nerves tibial velocity (whole SCP r = 0.43, p <0.01, parafoveal SCP r = 0.41, p <0.01), CMAP (parafoveal SCP r = -0.35, p<0.03), median DML (whole DC r = 0.36, p<0.03, foveal DCP r = 0.37, p<0.02) and in sensory median SNAP (whole SCP r = -0.31, p<0.05).

Conclusions
In adolescents with T1D without diabetic neuropathy and retinopathy we detected associations between NCS and OCT and OCTA parameters, regarding decreased GCC and density of superficial and deep vessel plexuses in relation to DML and CV and amplitudes of sensory and motor potential.
Impoverishment impact of out-of-pocket payments for healthcare in rural Bangladesh: Do the regions facing different climate change risks matter?
Authors: Begum Afroza , Hamid Syed Abdul 
Published: June 2021

Introduction
Out-of-pocket (OOP) payments for healthcare severely affect the current consumption, future health and earnings capacity of poor/underprivileged households and hence it is crucial for priority setting. This study assesses the variation in overall as well as disease-specific impoverishment impact of OOP payments between the regions experiencing different climate change risks, defined as high disaster-prone (HDP) areas and low-disaster-prone (LDP) areas, in Bangladesh.

Materials and methods
This paper estimated three poverty measures, such as poverty headcount, poverty intensity and normalized poverty gap for all ailments, catastrophic events, diseases types (communicable, non-communicable (NCDs), and accident and injury), illness conditions (acute and chronic) and hospitalization using 3,791 randomly selected rural households (1,203 from HDP and 2,588 from LDP areas) across the regions. Cost of basic need approach was used for estimating poverty line expenditure.

Results
About 13 percent households annually fall into poverty due to OOP outlays for healthcare. Despite having significantly (p-value≤0.01) less OOP payments (HDP areas: BDT 5,117; LDP areas: BDT5,811) the impoverishment impact of OOP payments for healthcare in HDP areas (16.5%) has substantially higher than LDP areas (11.3%).
Population in HDP areas, especially char (river island; 19.55 percent) and haor (water submerged; 16.80 percent) are more susceptible to any level of OOP payments due to low level of earnings. Catastrophic healthcare expenditure (61.79%) and NCDs (14.29 percent) are exacerbating the poverty level in Bangladesh. Both absolute and relative average poverty gap are more widen in HDP than LDP areas due to catastrophic OOP outlays for healthcare.

Conclusion
The impoverishment effect due to OOP payments for healthcare in both HDP and LDP areas are high, especially for NCDs and catastrophic healthcare expenditure. However, the situation is bit worse in HDP areas. Preventing the escalation of NCDs as well as catastrophic expenditure and hence reducing the level of impoverishment thereof call for restricting tobacco use, increasing physical activity, encouraging to intake healthy diets, ensuring food safety, controlling air pollution, and improving mental health. Moreover, government should give more emphasis, especially in the HDP areas, on making community clinics more functional through providing screening equipment and training to the Community Health Care Providers for early detection of NCDs, and ensuring availability of medicine all the time. Note that other than community clinics, there is little option for providing healthcare in HDP areas due to poor functionality of public facilities as well as lack of private facilities in HDP areas.
Neoadjuvant immunotherapy for resectable esophageal cancer: A protocol of meta-analysis
Authors: Yu Guocan , Yu Wenfeng , Xu Xudong , Ye Bo , Yao Liwei 
Published: June 2021

Background
Esophageal cancer is a highly malignant cancer with a very poor prognosis. For resectable esophageal cancer, neoadjuvant treatment could improve the prognosis of esophageal cancer. However, current clinical neoadjuvant treatment options for esophageal cancer are still limited. The application of immunotherapy is a potentially beneficial new neoadjuvant treatment option for esophageal cancer. The objective of this meta-analysis is to evaluate the efficacy and safety of immunotherapy for the neoadjuvant treatment of esophageal cancer.

Methods
We will search Wanfang Database, SinoMed, China National Knowledge Infrastructure, Embase, Web of Science, Pubmed, and Cochrane Library for relevant articles published before July, 2021. We will also search the unpublished clinical trials of neoadjuvant immunotherapy in esophageal cancer in preprint website (such as bioRXiv and medRxiv) up to July, 2021. We will perform a meta-analysis to evaluate the efficacy and safety of neoadjuvant immunotherapy for resectable esophageal cancer. Randomized controlled trials (RCTs) will be included in this study. The risk of bias will be evaluated for each included study using the Cochrane Handbook for Systematic Reviews of Interventions. We will use RevMan 5.3 software for statistical analysis of the data.

Results
The results of this study will provide evidence of immunotherapy using as neoadjuvant treatment for esophageal cancer. This meta-analysis will be submitted to a peer-reviewed journal seeking for publication.

Conclusion
The results of this study will provide a reliable basis for clinicians and patients to formulate the best pre-surgical treatment plan for resectable esophageal cancer.

Systematic review registration
INPLASY202120026.
Identification of leaky gut-related markers as indicators of metabolic health in Dutch adults: The Nutrition Questionnaires plus (NQplus) study
Authors: Hoshiko Hiroyuki , Feskens Edith J. M. , Oosterink Els , Ariens Renata M. C. , Mes Jurriaan J. , de Wit Nicole J. W. 
Published: June 2021

Background and aim
Chronic inflammation is a primary risk factor for chronic metabolic disease and may be triggered by a “leaky gut.” Several biomarkers have been recognized to indicate intestinal permeability (i.e., leaky gut) and bacterial translocation. Nonetheless, which of these biomarkers exhibit the highest correlation with metabolic health parameters remains unclear. Hence, this study aimed to explore the correlation between leaky gut-related markers and metabolic health.

Methods
Based on waist circumference, plasma fasting glucose, plasma gamma-glutamyl transpeptidase (GGT), and plasma LDL cholesterol, two groups of 40 subjects with the most extreme metabolic health profiles were selected from the NQplus cohort study (n = 2048), which was previously conducted by the Wageningen University’s Division of Human Nutrition. Eight potential leaky gut-related markers were selected from the literature and measured in serum or EDTA plasma samples of these selected individuals. These samples were also obtained from the NQplus cohort study.

Results
From the leaky gut markers, levels of zonulin, lipopolysaccharide-binding protein, soluble CD14, bactericidal/permeability-increasing protein, and peptidoglycan were significantly higher in individuals with unhealthy metabolic profiles (p<0.05). No differences in EndoCAb IgM, EndoCAb IgA, and EndoCAb IgG were observed between healthy and unhealthy individuals. Stepwise regression analysis revealed that zonulin was substantially associated with metabolic health parameters such as BMI, blood glucose, triglyceride, GGT, and C-reactive protein levels. C-reactive protein, an inflammation marker, showed the most pronounced association with zonulin.

Conclusions
Biomarkers that link a leaky gut and subsequent bacterial translocation to metabolic health were identified in this study. Especially zonulin may aid in monitoring a leaky gut and detecting individuals at risk for developing chronic metabolic diseases.
Extended measuring depth dual-wavelength Fourier domain optical coherence tomography
Authors: Al-Mohamedi Haroun , Kelly-Pérez Ismael , Oltrup Theo , Cayless Alan , Bende Thomas 
Published: June 2021

Abstract
In this work an enhanced wide range dual band spectral domain optical coherence tomography technique (SD-OCT) is presented to increase the depth and accuracy of the measurement of optical A-scan biometry. The setup uses a Michelson interferometer with two wide-spectrum Superluminescent Diodes (SLD). The emissions of the SLDs are filtered by a long-pass filter (900 nm) in front of the reference mirror. The light is spectrally decomposed using a single reflective diffraction grating (1,800 lines/mm) and the whole spectrum captured with two CCD line sensors. The capabilities of the system have been validated using a self-made human model eye.
Multi-scale evaluation of the effect of saturated steam on the micromechanical properties of Moso bamboo
Authors: Yuan Tiancheng , Wang Zhaoshun , Han Xin , Yuan ZhuRun , Wang XinZhou , Li Yanjun 
Published: June 2021

Abstract
The changes in chemical composition and micro-mechanical properties of Moso Bamboo fiber cells were evaluated by applying saturated steam heat treatment at 160, 170, and 180 °C for periods of 4, 6, and 8 min, and subsequent analysis by X-ray diffraction, Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopic, and nanoindentation methods. The hemicellulose and cellulose content decreased as expected, while the relative lignin content showed an upward trend. Significant changes in the bamboo micro-structure were detected by scanning electron microscope under the action of high-temperature and saturated steam. Both temperature and time were shown to affect micromechanical properties of the bamboo cell wall. In addition, higher cell wall elastic modulus and hardness were observed (a rise from 16.1 to 19.2 GPa and from 0.6 to 0.8 GPa, respectively), as compared with those of the untreated sample. Meanwhile, the creep ratio decreased after saturated steam heat treatment.
Extractives in Betula celtiberica stemwood and isolation and identification of diarylheptanoids in the hydrophilic extract
Authors: Smeds Annika I. , Herrera René , Rahkila Jani , Willför Stefan M. 
Published: June 2021

Abstract
In Betula celtiberica (Iberian white birch) stemwood, the content and composition of lipophilic and hydrophilic extractives were determined; these have not been reported previously in this species. The total gravimetric amount of extractives was 2.2% of dry wood, of which lipophilic extractives accounted for 0.39% and hydrophilic extractives 1.84% (determined by gas chromatography–flame ionisation detection [GC–FID]). The lipophilic extract contained mainly triterpenoids and steroids. The hydrophilic extract (acetone–water 95:5 v v−1) contained mainly sugars, compounds not eluting on GC, and a large number of unidentified compounds, which accounted for 0.87% of dry wood and dominated the extract. The compounds were isolated from the extract by silica column chromatography and further purified. GC–electron impact (EI)-MS of the silylated compounds showed characteristic mass fragments that suggested them to be diarylheptanoids (DAHs). This is an interesting compound group, as many of them have shown a large variety of beneficial biological effects. Of over 80 detected DAHs, the exact mass of 17 compounds was determined by electrospray ionisation-quadrupole-time-of-flight (ESI-QTOF)-MS, and of these, the structure of 11 compounds was elucidated by nuclear magnetic resonance (NMR). One was a meta,para-bridged diphenylether and 10 were meta,meta-bridged biphenyls, of which one, 3,8,9,17-tetrahydroxy-[7,0]-metacyclophane, has not been described previously. Because of low concentrations, 21 DAHs were only tentatively identified, and of these, as many as 17 seem to be previously undescribed compounds.
Lymphotoxin-β-receptor (LTβR) signaling on hepatocytes is required for liver regeneration after partial hepatectomy
Authors: Sorg Ursula R. , Küpper Nicole , Mock Julia , Tersteegen Anne , Petzsch Patrick , Köhrer Karl , Hehlgans Thomas , Pfeffer Klaus 
Published: June 2021

Abstract
Lymphotoxin-β-receptor deficient (LTβR−/−) and Tumor Necrosis Factor Receptor p55 deficient (TNFRp55−/−) mice show defects in liver regeneration (LR) after partial hepatectomy (PHx) with significantly increased mortality. LTβR and TNFRp55 belong to the core members of the TNF/TNFR superfamily. Interestingly, combined failure of LTβR and TNFRp55 signaling after PHx leads to a complete defect in LR. Here, we first addressed the question which liver cell population crucially requires LTβR signaling for efficient LR. To this end, mice with a conditionally targeted LTβR allele (LTβRfl/fl) were crossed to AlbuminCre and LysozymeMCre mouse lines to unravel the function of the LTβR on hepatocytes and monocytes/macrophages/Kupffer cells, respectively. Analysis of these mouse lines clearly reveals that LTβR is required on hepatocytes for efficient LR while no deficit in LR was found in LTβRfl/fl × LysMCre mice. Second, the molecular basis for the cooperating role of LTβR and TNFRp55 signaling pathways in LR was investigated by transcriptome analysis of etanercept treated LTβR−/− (LTβR−/−/ET) mice. Bioinformatic analysis and subsequent verification by qRT-PCR identified novel target genes (Cyclin-L2, Fas-Binding factor 1, interferon-related developmental regulator 1, Leucyl-tRNA Synthetase 2, and galectin-4) that are upregulated by LTβR/TNFRp55 signaling after PHx and fail to be upregulated after PHx in LTβR−/−/ET mice.
Ancient Latin grammarians on suppletion
Authors: Pultrová Lucie 
Published: June 2021

Abstract
The term “suppletion”, introduced by Osthoff (1899. Vom Suppletivwesen der indogermanischen Sprachen. Heidelberg: Universitätsbuchdruckerei Hörning), was traditionally used to refer to an inflectional paradigm containing forms based on two or more etymologically different stems. In the last decades, however, it has been argued that etymology does not contribute to our understanding of the phenomenon, and it should be strictly defined on synchronic terms: simply as the peak point on the formal irregularity scale, regardless of the actual origin of the irregularity. Under this approach, all forms reported by speakers as two potentially different lexical items are considered to be suppletive. To be able to determine what users of a living language consider to be a case of suppletion, it is possible to analyze data collected from speakers. The situation is considerably more difficult for dead languages, which however have played an important role in the debate and provided many of the canonical examples. As a closest equivalent to eliciting the required information from a native speaker, the informed but from the present-day perspective naïve expressions of linguistic introspection in the works of Late Latin Grammarians, namely their use of specific terms (defectivum, anomalum, inaequale) to refer to different degrees and lexical examples of irregularity, are highly valuable, as it also may reflect the difficulties confronted by non-native learners.
The effect of postnatal corticosteroids on growth parameters in infants with bronchopulmonary dysplasia
Authors: Williams Emma E. , Dassios Theodore , Mann Mikhaela , Greenough Anne 
Published: June 2021

Abstract

Objectives
Corticosteroids are administered to ventilator dependent infants with bronchopulmonary dysplasia (BPD) to improve respiratory function and facilitating extubation. Acutely, however, growth impairment can occur as a side effect of such therapy. We aimed to determine the effect of corticosteroids on postnatal growth during the entire neonatal intensive care unit (NICU) admission.

Methods
A whole population study of extremely preterm infants with BPD was undertaken. Corticosteroid therapy was classified as treatment with dexamethasone or hydrocortisone for a least five consecutive days. Growth was calculated as the difference in weight and head circumference z-score from birth to discharge.

Results
Six thousand, one hundred and four infants with BPD were included of whom 28.3% received postnatal corticosteroids. Infants receiving corticosteroids were less mature (GA 25.0 vs. 26.3 weeks) and of lower birthweight (0.70 vs. 0.84 kg) than those not receiving treatment. There were no significant differences between those who did and did not receive corticosteroids in weight gain (p=0.61) or head circumference growth (p=0.33) from birth to discharge. Single vs. multiple courses of postnatal corticosteroids did not result in significant differences in weight (p=0.62) or head circumference (p=0.13) growth.

Conclusions
Postnatal corticosteroid treatment did not affect the longer term growth of preterm infants with BPD.