bluuebunny/crossref_metadata_2025_split huggingface dataset:
How human serum albumin‐selective DNA aptamer binds to bovine and canine serum albumins
Authors: Kuntip Nattapon , Japrung Deanpen , Pongprayoon Prapasiri 
Published: February 2021

AbstractSerum albumin (SA) is the most abundant carrier protein in blood. SA carries a diverse range of nutrients, drugs, and metal ions. It has wide clinical and biochemical applications. Human serum albumin (HSA) can be used as a biomarker for kidney and liver diseases. Aptasensor is one of potential HSA detection methods. HSA‐specific aptamer was selected for HSA detection. In animals, bovine serum albumin (BSA) and canine serum albumins (CSA) share high sequence similarities to HSA. Thus, it is interesting to explore the possibility of using HSA‐selective aptamer for BSA and CSA aptasensor. In this study, molecular dynamics (MD) simulations were initially employed to investigate the binding of aptamer to BSA and CSA in comparison to HSA. Like HSA, both BSA and CSA can bind aptamer, but different binding affinities are observed. BSA shows the tighter binding to aptamer than CSA. Domain III is found to be the aptamer‐binding domain although no specific aptamer conformation is captured. However, in all cases, the aptamer utilizes the 3′‐end to attach on an albumin surface. Both nucleobases and phosphate backbones on a DNA aptamer are important for albumin‐aptamer complexation. Our results imply the possibility of using HSA‐specific aptamer for BSA detection due to tighter binding observed, but may be less effective in CSA. However, the test in actual complicated condition must be further studied.
Varietal Differences of Prokupac, Evita and Čokot Zemun Based on Their Anthocyanins Content in Grape Skin Extract
Authors: Živković Jelena , Jadranin Milka , Pržić Zoran , Marković Nebojša , Sokolović Danijel , Šavikin Katarina , Menković Nebojša 
Published: February 2021

AbstractIn this study, we have analyzed the anthocyanin composition of skin extracts of three red grape varieties Prokupac, Evita and Čokot Zemun in order to distinguish these cultivars based on their anthocyanin profile. Also, mechanical analysis of grape bunches and berries was performed. According to our results, seventeen anthocyanins were identified using LC/MS technique and quantitative differences were recorded using HPLC‐DAD method. The highest content of total anthocyanins was obtained for Evita variety and the lowest one was recorded in Prokupac. Also, clear differences were observed in anthocyanins ratios. In comparison to Prokupac and Evita varieties, Čokot Zemun was characterized with a high content of coumaroyl derivatives of anthocyanin compounds, while high levels of acetylated derivatives were recorded in Prokupac. Data reported in this study represent a certain contribution to a database of mechanical properties and chemical composition of grape varieties originating from Balkan.
Effect of institutional transcatheter aortic valve replacement volume on mortality: A systematic review and meta‐analysis
Authors: Kir Devika , Shapero Kayle , Chatterjee Saurav , Grimshaw Alyssa , Oddleifson August , Spatz Erica S. , Goldsweig Andrew M. , Desai Nihar R. 
Published: February 2021

AbstractObjectiveWe sought to conduct a systematic review and network meta‐analysis to examine the association between institutional transcatheter aortic valve replacement (TAVR) volume and all‐cause mortality.BackgroundSince inception in 2011, there has been an exponential increase in the number of TAVR centers across the world. Multiple studies have questioned if a relationship exists between institutional TAVR volume and patient outcomes.MethodsWe performed a systematic literature search for relevant articles using a combination of free text terms in the title/abstract related to volume, TAVR, and patient outcomes. Two reviewers independently screened all titles/abstracts for eligibility based on pre‐specified criteria. All‐cause mortality data was pooled from eligible studies and centers were categorized as low‐(30–50 cases), intermediate‐, or high‐volume (75–130 cases) based on their annual TAVR volumes.ResultsOur search yielded an initial list of 11,153 citations, 120 full text studies were reviewed and 7 studies met all inclusion and exclusion criteria, yielding a total of 1,93,498 TAVRs. Categorized according to center's annual volume; 25,062 TAVRs were performed in low‐, 77,093 in intermediate‐ and 91,343 in high‐volume centers. Network meta‐analysis showed a relative reduction in mortality rates of 37%, 23% and 19%, for high volume versus low volume centers, high volume versus intermediate volume centers and intermediate versus low volume centers, respectively.ConclusionsExisting research clearly shows an inverse relationship between annual TAVR procedural volume and all‐cause mortality. We need to focus on development of strong referral networks and consolidation rather than expansion of existing TAVR centers to improve patient outcomes, while ensuring adequate access‐to‐care.
Usability of Fantom Encore® scaffold in non‐complex bifurcations–Analysis in bench models
Authors: G. Toth Gabor , Ono Masafumi , Buschmann Eva , Wu Xinlei , Edl Dominik , Onuma Yoshinobu , Serruys Patrick WJC , Wijns William 
Published: February 2021

AbstractObjectivePresent bench study aimed to evaluate whether technical characteristics of Fantom Encore® bioresorbable scaffold (BRS) allow to perform proximal optimization/side branch dilation/proximal optimization (POT‐SB‐POT) technique, as an adequate solution for bifurcation percutaneous coronary intervention.MethodsTwo Fantom Encore® BRS platforms (small with 3.0 mm nominal diameter, n = 7; and large with 3.5 mm nominal diameter, n = 7) were evaluated in bench models, which were designed according to Finet‐law and fitted to nominal scaffold diameter in the distal main branch (MB) and fitted to indicated maximal expansion capacity in the proximal main branch (MB). Results were evaluated by (a) fluoroscopy, (b) optical coherence tomography (OCT) and (c) micro‐computed tomography (μCT).ResultsAll procedures were performed according to the protocol. Careful review of the fluoroscopic loops by an independent operator did not reveal any strut fracture or major deformation. By OCT the overall rate of perfectly apposed struts in the bifurcation area was 15 ± 6% after SB opening, that increased significantly but remained low with 22 ± 9% after final POT (p = .001). Compared to SB ballooning alone, significant benefit of final POT was found in rate of perfect apposition in the proximal MB (15 ± 12% vs. 26 ± 15%, respectively; p = .017) and at the abostial side of polygon of confluence (7 ± 9% vs. 16 ± 13%, respectively; p = .005).μCT analysis revealed a single strut fracture in one case with the small platform, while four cases showed single or multiple strut fractures with the large platform.ConclusionThe mechanical characteristics of the device are not suitable for use of Conventional techniques for bifurcation PCI such as POT‐SB‐POT. The use of Fantom Encore® BRS for bifurcation PCI with relevant SB should not be encouraged.
Aortic stenosis in the time of COVID‐19: Development and outcomes of a rapid turnaround TAVI service
Authors: Adlam David , Chan Nathan , Baron Julia , Kovac Jan 
Published: February 2021

AbstractThe COVID‐19 pandemic has resulted in the cancellation of many elective surgical procedures. This has led to reports of an increase in mortality for patients with non‐Covid health conditions due to delayed definitive management. Patients with severe aortic stenosis have a high annual mortality if left untreated. These patients are at risk due to the reduced number of surgical aortic valve replacements and competition for intensive care facilities during the COVID‐19 pandemic. This case series suggests that the minimally invasive transcatheter aortic valve implantation is safe to continue during the COVID‐19 pandemic with adjustments to the patient pathway to minimize hospital stay and to reduce patient and staff exposure. This helps to reduce the delay of definitive treatment for patients with severe aortic stenosis.
NTRK2 Fusion driven pediatric glioblastoma: Identification of oncogenic Drivers via integrative Genome and transcriptome profiling
Authors: Britton Heidi M. , Levine Adrian B. , Shen Yaoqing , Mungall Karen , Serrano Jonathan , Snuderl Matija , Pleasance Erin , Jones Steven J. M. , Laskin Janessa , Marra Marco A. , Rassekh Shahrad R. , Deyell Rebecca , Yip Stephen , Cheng Sylvia , Dunham Chris 
Published: February 2021

AbstractThis is the first report of a NACC2‐NTRK2 fusion in a histological glioblastoma. Oncogenomic analysis revealed this actionable fusion oncogene in a pediatric cerebellar glioblastoma, which would not have been identified through routine diagnostics, demonstrating the value of clinical genome profiling in cancer care.
Ischemic colitis and atrial septal aneurysm as a potential cause of systemic thromboembolism
Authors: Subahi Eihab A. , Kumar Narinder , Hamid Abdulwahab M. , Elmahadi Mohamed S. , Yassin Mohamed A. 
Published: February 2021

AbstractAtrial septal aneurysm is a rare cardiac abnormality with thromboembolic potential and should be considered in a patient with ischemic colitis with no obvious risk factors.
Use of the NobleStitch™ EL for the treatment of patients with residual right‐to‐left shunt following device closure of PFO
Authors: Chaudhry‐Waterman Nadia , Shapiro Stephen , Thompson James 
Published: February 2021

AbstractThe unique design of the NobleStitch™ EL allows it to be used to close residual defects following failed device PFO closure without impacting the integrity of previously placed double‐disk Gore occluders.
Abdominal aortic thrombus formation in a neonate with an interrupted aortic arch
Authors: Yuhara Yuka , Kido Takahiro , Imagawa Kazuo , Yano Yusuke , Nozaki Yoshihiro , Ishiodori Takumi , Ishikawa Nobuyuki , Kato Hideyuki , Kato Yoshiaki , Takahashi‐Igari Miho , Murakami Takashi , Horigome Hitoshi , Takada Hidetoshi 
Published: February 2021

AbstractWe note the risk of paradoxical embolism in patients with congenital heart defects with a right‐to‐left shunt. These patients should be managed to ensure that abdominal aortic thrombi are not overlooked when their clinical conditions change.
A neonate born to an infected COVID‐19 mother was tested positive just 24 hours after its birth
Authors: Huseynova Roya Arif , A. Bin Mahmoud Latifa , Huseynov Oqtay , Almalkey Mishary , Amer Almotiri Ameen , Sumaily Haider Hussein , AbdelRahim Adli 
Published: February 2021

AbstractGiven the importance of possible vertical transmission, it is essential to study all neonates delivered from COVID‐19 positive mothers to analyze the route of transmission of infection that will optimize the universal testing for SARS‐CoV‐2 and minimize the risk of disease in neonates.
Modelling and validation of a gas‐solid fluidized bed using advanced measurement techniques
Authors: Uribe Sebastián , Taofeeq Haidar , Al‐Dahhan Muthanna 
Published: February 2021

AbstractWith a Euler‐two‐phase (E2P) approach, through computational fluid dynamics (CFD) techniques, a mathematical model for the prediction of the local hydrodynamic behaviour of a gas‐solid fluidized bed was implemented. Simulations are conducted for a fluidized bed of 0.14 m internal diameter packed with Gerdart B glass beads particles, with an average diameter of 365 μm, at dimensionless inlet velocities ranging from . The implemented model considers the multiphase and multiscale interactions through the inclusion of three sub‐models, which allows the model to have a broad range of applicability. Predictions were compared against experimental measurements reported on previous contributions for validation purposes. The experimental study was conducted by implementing advanced measurement techniques, such as a differential pressure transducer, and an optical fibre probe for simultaneous measurement of solids holdup and velocity, developed at the Multiphase Flow and Reactors Engineering and Applications Laboratory (mFReal). Local radial solids holdup, solids velocity, and pressure drop profiles were experimentally determined. Results show that the implemented model possesses a high predictive quality, predicting pressure drops with an average absolute relative error (AARE) between 8.6%–11.3%; solids holdup with a root mean squared deviation (RMSD) under 5%; and solids velocity with a RMSD under 22%.
Data‐driven hypotheses of reaction networks for thermochemical conversion of a physical mixture of levoglucosan and 2‐phenoxyethyl benzene
Authors: Sattari Fereshteh , Prasad Vinay 
Published: February 2021

AbstractIn this work, we analyze the hydrous pyrolysis of a physical mixture of the model components representing cellulose (levoglucosan) and lignin (2‐phenoxyethyl benzene). Fourier transform infrared (FTIR) and proton nuclear magnetic resonance (1H‐NMR) spectroscopy was used to characterize the products of the reaction. The main objective of the work was to use data‐driven methods to develop a reaction network for this system based on the spectroscopic data. This was achieved using Bayesian hierarchical clustering to identify pseudocomponents and Bayesian networks to develop a reaction network between these pseudocomponents. The data‐driven reaction network was shown to be consistent with the known chemistry of the pyrolysis of cellulose and lignin, and the chemistry of the physical mixture incorporated/combined elements of the reaction mechanisms of cellulose and lignin.
Obtaining Kinetics From Continuous Processes: Sampling Multiple Time Points Concurrently With a Single Valve Rotation
Authors: Morin Mathieu A. , Mallik Debasis , Zhang Wenyao , Pietro William , Manthorpe Jeffrey M. , Organ Michael G. 
Published: February 2021

AbstractThe manufacturers of fine chemicals and pharmaceuticals are beginning to accept flow chemistry as a production alternative following the lead of commodity chemical manufacturers, who have been producing goods in continuous format for decades. The ability to trace time backwards by retracting the spatial length of a flow reactor is perhaps one of the biggest advantages of flowed synthesis. However, in the absence of an appropriate tool that would allow scientists to sample throughout the entire space of a production line, the theory is not possible to put into practice. A step in this direction has been taken with the invention of a unique, multi‐port valve/flow reactor that allows the chemist to simultaneously sample multiple timepoints of a chemical transformation with one single valve actuation, thereby capturing instantaneously the entire reaction course of the flowed chemical process.
Forage accumulation and radiation use of alfalfa under deficit irrigation
Authors: Liu Minguo , Mu Le , Lu Yixiao , Yang Huimin 
Published: February 2021

AbstractThe supply of high‐quality forage is fundamental to the development of ruminant animal production in drought areas where irrigation provides strong support to the forage production. The objective was to explore the response of alfalfa (Medicago sativa L.) growth and radiation use to deficit irrigation in a 2‐yr field study. Two deficit‐irrigation treatments and a full‐irrigation control (CK) were established under flood irrigation. Growth‐related indicators and radiation use were measured regularly. Results showed that deficit irrigation reduced forage accumulation, leaf area index (LAI), plant relative water content (RWC), and radiation use efficiency (RUE) of alfalfa. Compared with CK, slight deficit irrigation (SD) and moderate deficit irrigation (MD) decreased forage accumulation rate by 20 and 30%, respectively. Logistic model as a function of growing degree days (GDD) fitted well LAI change (R2 > .9). Deficit irrigation reduced the average rate of quick growth of LAI from 5 to 36%. Water deficit did not significantly change the GDD corresponding to the rapid growth phase but reduced the growth rate. Under deficit irrigation, decreased LAI did not always lead to significant cumulative intercepted photosynthetically active radiation (CIPAR) decrease, but resulted in significantly lowered RUE. In conclusion, the decrease of forage accumulation of alfalfa under deficit irrigation would be caused mainly by weakened photosynthetic ability (representing RUE).
Identification of Hypoxia‐inducible factor (HIF) stabilizer roxadustat and its possible metabolites in thoroughbred horses for doping control
Authors: Mathew Binoy , Philip Moses , Perwad Zubair , Karatt Tajudheen K. , Caveney Marina Rodriguez , Subhahar Michael Benedict , Karakka Kal Abdul Khader 
Published: February 2021

AbstractHypoxia‐inducible factor (HIF) stabilizer belongs to a novel class of pharmacologically active substances, which are capable of inducing the endogenous erythropoietic system. The transcriptional activator HIF has been shown to significantly increase blood hemoglobin and is well set for the treatment of anemia resulting from chronic kidney disease. This research work reports a comprehensive study of the most popular HIF stabilizer roxadustat and its metabolites in thoroughbred horse urine after oral administration. The plausible structures of the detected metabolites were postulated using liquid chromatography‐high‐resolution mass spectrometry. Under the experimental condition 13 metabolites (7 phase I, 1 phase II, and 5 conjugates of phase I metabolism) were positively detected (M1–M13). The major phase I metabolites identified were formed by hydroxylation. Dealkylated and hydrolyzed phase I metabolites were also observed in this study. In phase II, a glucuronic acid conjugate of roxadustat was detected as the major metabolite. The sulfonic acid conjugates were observed to be formed from phase I metabolites. The characterized in vivo metabolites can potentially serve as target analytes for doping control analysis; hence, the result is an important tool for assessing its use and abuse in competitive sport.
Retinol binding protein 1 affects Xenopus anterior neural development via all‐trans retinoic acid signaling
Authors: Flach Hannah , Basten Thomas , Schreiner Corinna , Dietmann Petra , Greco Sara , Nies Lea , Roßmanith Nathalie , Walter Svenja , Kühl Michael , Kühl Susanne J. 
Published: February 2021

AbstractBackgroundRetinol binding protein 1 (Rbp1) acts as an intracellular regulator of vitamin A metabolism and retinoid transport. In mice, Rbp1 deficiency decreases the capacity of hepatic stellate cells to take up all‐trans retinol and sustain retinyl ester stores. Furthermore, Rbp1 is crucial for visual capacity. Although the function of Rbp1 has been studied in the mature eye, its role during early anterior neural development has not yet been investigated in detail.ResultsWe showed that rbp1 is expressed in the eye, anterior neural crest cells (NCCs) and prosencephalon of the South African clawed frog Xenopus laevis. Rbp1 knockdown led to defects in eye formation, including microphthalmia and disorganized retinal lamination, and to disturbed induction and differentiation of the eye field, as shown by decreased rax and pax6 expression. Furthermore, it resulted in reduced rax expression in the prosencephalon and affected cranial cartilage. Rbp1 inhibition also interfered with neural crest induction and migration, as shown by twist and slug. Moreover, it led to a significant reduction of the all‐trans retinoic acid target gene pitx2 in NCC‐derived periocular mesenchyme. The Rbp1 knockdown phenotypes were rescued by pitx2 RNA co‐injection.ConclusionRbp1 is crucial for the development of the anterior neural tissue.
Optimization and validation of a cost‐effective protocol for biosurveillance of invasive alien species
Authors: Milián‐García Yoamel , Young Robert , Madden Mary , Bullas‐Appleton Erin , Hanner Robert H. 
Published: February 2021

AbstractEnvironmental DNA (eDNA) metabarcoding has revolutionized biodiversity monitoring and invasive pest biosurveillance programs. The introduction of insect pests considered invasive alien species (IAS) into a non‐native range poses a threat to native plant health. The early detection of IAS can allow for prompt actions by regulating authorities, thereby mitigating their impacts. In the present study, we optimized and validated a fast and cost‐effective eDNA metabarcoding protocol for biosurveillance of IAS and characterization of insect and microorganism diversity. Forty‐eight traps were placed, following the CFIA's annual forest insect trapping survey, at four locations in southern Ontario that are high risk for forest IAS. We collected insects and eDNA samples using Lindgren funnel traps that contained a saturated salt (NaCl) solution in the collection jar. Using cytochrome c oxidase I (COI) as a molecular marker, a modified Illumina protocol effectively identified 2,535 Barcode Index Numbers (BINs). BINs were distributed among 57 Orders and 304 Families, with the vast majority being arthropods. Two IAS (Agrilus planipennis and Lymantria dispar) are regulated by the Canadian Food Inspection Agency (CFIA) as plant health pests, are known to occur in the study area, and were identified through eDNA in collected traps. Similarly, using 16S ribosomal RNA and nuclear ribosomal internal transcribed spacer (ITS), five bacterial and three fungal genera, which contain species of regulatory concern across several Canadian jurisdictions, were recovered from all sampling locations. Our study results reaffirm the effectiveness and importance of integrating eDNA metabarcoding as part of identification protocols in biosurveillance programs.
Plant species with the trait of continuous flowering do not hold core roles in a Neotropical lowland plant‐pollinating insect network
Authors: Hinton Chelsea R. , Peters Valerie E. 
Published: February 2021

AbstractPlant–animal interaction science repeatedly finds that plant species differ by orders of magnitude in the number of interactions they support. The identification of plant species that play key structural roles in plant–animal networks is a global conservation priority; however, in hyperdiverse systems such as tropical forests, empirical datasets are scarce. Plant species with longer reproductive seasons are posited to support more interactions compared to plant species with shorter reproductive seasons but this hypothesis has not been evaluated for plant species with the longest reproductive season possible at the individual plant level, the continuous reproductive phenology. Resource predictability is also associated with promoting specialization, and therefore, continuous reproduction may instead favor specialist interactions. Here, we use quantitative pollinating insect–plant networks constructed from countryside habitat of the Tropical Wet forest Life Zone and modularity analysis to test whether plant species that share the trait of continuous flowering hold core roles in mutualistic networks. With a few exceptions, most plant species sampled within our network were assigned to the role of peripheral. All but one network had significantly high modularity scores and each continuous flowering plant species was in a different module. Our work reveals that the continuous flowering plant species differed in some networks in their topological role, and that more evidence was found for the phenology to support specialized subsets of interactions. Our findings suggest that the conservation of Neotropical pollinating insect communities may require planting species from each module rather than identifying and conserving network hubs.