bluuebunny/crossref_metadata_2025_split huggingface dataset:
MMP1 is a Promiseful Prognostic Biomarker and Correlating with Immune Infiltrates in Hepatocellular Carcinoma
Authors: Dai Lei , Muggnyi Joseph , cai xingchen , mao shuqi , zhang tongyue , shi haihong , lu caide , lu changjiang 
Published: June 2021

Abstract
Background: Recombinase-aided amplification(RAA) is a new, simple, and ultrafast isothermal molecular diagnostic technique performed within 30min at 39°C–42°C.In this study, we evaluated the clinical performance of four duplex RAA kits for hepatitis B virus(HBV), human adenovirus 3(HAdV3), human adenovirus 7(HAdV7), and Bordetella pertussis and one duplex reverse-transcription RAA (RT-RAA) kit for respiratory syncytial virus (RSV).Methods: A total of 392 sera and 374 respiratory tract samples were collected from five institutions in four China regions. Each RAA kit’s sensitivity and specificity were compared with those of real-time quantitative polymerase chain reaction(qPCR),real-time quantitative reverse-transcription polymerase chain reaction(qRT-PCR), or sequencing. Results: Compared with qPCR or qRT-PCR, the sensitivities of HBV RAA,RSV RT-RAA, and B.pertussis RAA were 97.55%,96.67%, and 100%,respectively,and all of the specificities were 100%.The total coincidence rates were 97.78%(383/392,95%CI:95.63%–98.85%),97.70%(212/217, 95%CI:94.57%–99.16%), and 100%(60/60,95%CI:92.80%–100%),respectively. The Kappa values were 0.977,0.947, and 1,respectively(P<0.05).Regarding the sequencing, the sensitivities of HAdV3 RAA and HAdV7 RAA were 100% and 97.37%, respectively, and all specificities were 100%.The total coincidence rates were 100%(97/97,95%CI:91.58%–100%) and 98.97%(96/97,95%CI:94.39%–99.82%),and the Kappa values were 1 and 0.978 (P<0.05),respectively.Conclusions: With comparable clinical performance, these RAA kits are suitable assays for rapidly detecting pathogens in resource-limited laboratories.
Melatonin Receptor 2 Promotes Lipid Metabolism of Cumulus-oocyte Complexes via the cAMP/PKA Pathway
Authors: Jin Jun-Xue , Cui Hong-Di , Jiang Chao-Qian , Qi Zi-Cheng , Bian Ya , Liu Jia-Hui , Jiang Xi-Qing , Sun Jing-Tao , Liu Zhong-Hua 
Published: June 2021

Abstract
Background: The importance of the processes of lipogenesis and lipolysis in providing an essential energy source during oocyte maturation is increasingly being recognized. Recent our studies have demonstrated that melatonin up-regulated lipid metabolism during oocyte maturation. Nevertheless, there is still limited information regarding the underlying molecular mechanisms of action of melatonin on lipid metabolism in porcine cumulus-oocyte complexes (COCs). Here, our aim was to investigate the effect of melatonin on COCs, and the melatonin receptor-mediated lipid metabolism signaling pathway.Materials/methods: To determine the melatonin-mediated lipolysis pathway in cumulus cells, COCs were treated with melatonin and the correlated metabolic responses were assessed using melatonin receptor-mediated signaling.Results: The results showed that exposure of COCs to melatonin during in vitro maturation significantly increased cumulus expansion index, blastocyst formation rate and total cell numbers/blastocyst, although nuclear maturation was no significant difference. The levels of proteins MT1, MT2, Gsα, PKA, and lipolysis-related factors (AGTL, HSL, PLIN A+B) were significantly increased by melatonin supplementation, and this effect was inhibited by simultaneous treatment with melatonin antagonists (luzindole or 4P-PDOT), although 4P-PDOT treatment did not completely block the effect of melatonin on MT1. Further, the gene expression patterns reflected their relevant protein levels in cumulus cells. Melatonin-mediated lipolysis could significantly reduce lipid droplets (LDs) numbers and increase fatty acid (FA) production and ATP levels by increasing the β-oxidation-related gene expression in cumulus cells. Simultaneously, melatonin significantly increased the amount of LDs, FAs, ATP, and enhanced the lipid metabolism-related gene expression in oocytes. Finally, the oocyte quality was improved by increasing GDF9, BMP15 and GSH and decreasing ROS levels.Conclusion: These findings revealed that the MT2-mediated cAMP/PKA signaling pathway promotes intracellular lipolysis and FA production in cumulus cells, which provided an essential energy source for COCs development.
GOLGA2/GM130 is a Novel Target for Neuroprotection Therapy in Intracerebral Hemorrhage
Authors: Deng Shuwen , Hu Qing , He Qiang , Chen Xiqian , Lu Wei 
Published: June 2021

Abstract
Blood–brain barrier (BBB) impairment after intracerebral hemorrhage (ICH) can lead to secondary brain injury and aggravate neurological deficits. Currently, there are no effective methods for its prevention or treatment partly because of to our lack of understanding of the mechanism of ICH injury to the BBB. Here, we explored the role of Golgi apparatus protein GM130 in the BBB and neurological function after ICH. The levels of the tight junction-associated proteins ZO-1 and occludin decreased, whereas those of LC3-II, an autophagosome marker, increased in hemin-treated Bend.3 cells (p < 0.05). Additionally, GM130 overexpression increased ZO-1 and occludin levels, while decreasing LC3-II levels (p < 0.05). GM130 silencing reversed these effects and mimicked the effect of hemin treatment (p < 0.05). Moreover, tight junctions were disrupted after hemin treatment or GM130 silencing and repaired by GM130 overexpression. GM130 silencing in Bend.3 cells increased autophagic flux, whereas GM130 overexpression downregulated this activity. Furthermore, GM130 silencing-induced tight junction disruption was partially restored by 3-methyladenine (an autophagy inhibitor) administration. Similarly, an in vivo ICH rat model showed elevated perihematomal ZO-1 and occludin expression and decreased LC3-II expression (p < 0.05); these results were reversed following GM130 silencing (p < 0.05). Perihematomal Evans Blue staining and brain water content were elevated in GM130-silenced ICH rats relative to control ICH rats. GM130 overexpression can protect BBB integrity from brain injury, inhibit excessive autophagy flux in ICH, and improve neurobehavioral prognosis. Therefore, therapy targeting GM130 regulation might represent a potential treatment for acute brain injury after ICH.
Are Cut-off Lows Simulated Better in CMIP6 Compared to CMIP5 ?
Authors: Pinheiro Henri , Ambrizzi Tercio , Hodges Kevin , Gan Manoel , Andrade Kelen , Garcia Jose 
Published: June 2021

Abstract
This is the first study to show the global Cut-off Low (COL) activity in 23 models participating in the Coupled Model Intercomparison Project Phase 5 (CMIP5) and 17 models from Phase 6 (CMIP6). The COL historical simulations for the period 1979-2005 obtained from the CMIP5 and CMIP6 models and their ensembles are compared with the ERA5 reanalysis using an objective feature-tracking algorithm. The results show that the CMIP6 models simulate the spatial distribution of COLs more realistically than the CMIP5 models. Some improvements include reduced equatorward bias and underestimation over regions of high COL density. Reduced biases in CMIP6 are mainly attributed to the improved representation of the zonal wind due to the poleward shift of the subtropical jet streams. The CMIP5 models systematically underestimate the COL intensity as measured by the T42 vorticity at 250 hPa. In CMIP6, the intensity is still underestimated in summer, but overestimated in winter in part due to increased westerlies. The overestimation is enhanced by the finer spatial resolution models that identify more of the strong systems compared to coarser resolution models. Other aspects of COLs such as their temporal and lifetime distributions are modestly improved in CMIP6 compared to CMIP5. Finally, the predictive skill of climate models is evaluated using five variables and the Taylor diagram. We find that 10 out of the 15 best CMIP5-CMIP6 models belong to CMIP6, and this highlights the overall improvement compared to its predecessor CMIP5. Despite this, the use of the multi-model ensemble average seems to be better in simulating COLs than individual models.
The Risk Atlas of Mexico City, Mexico: A Tool for Decision-Making and Disaster Prevention
Authors: Novelo-Casanova David A. , Suárez Gerardo , Cabral-Cano Enrique , Fernández-Torres Enrique A. , Fuentes-Mariles Oscar A. , Havazli Emre , Jaimes Miguel Á. , López-Espinoza Erika D. , Martin-Del-Pozzo Ana Lillian , Morales-Barrera Wendy V. , Moráles-Rodríguez Hipólito L. , Nieto-Torres Amiel , Rodríguez-Elizarrarás Sergio , Solano-Rojas Darío , Velasco-Herrera Victor M. 
Published: June 2021

Abstract
We present a Geographical-Information-System-based Risk Atlas (GIS-RA) of Mexico City, Mexico. The main purposes are to provide a local-government level tool for implementing preventive and remedial actions useful for land-use development plans and to strengthen the culture for disaster prevention. We identified the prevalent social risk to the more relevant hazards in Mexico City (CDMX): earthquakes, volcanic eruptions, floods, landslides, forest fires, and land subsidence. A total of 274 shape-file maps were generated in this project. Seismic hazard was estimated for return periods (RP) of 20, 125, 250, and 475 years. Three areas in central and northwestern CDMX were identified along the Younger Chichinautzin Monogenetic Volcanic Field with high probability of forming a new volcano. Subsidence is concentrated to the east and southeast of CDMX, where subsidence rates are among the highest worldwide. Flooding events were estimated for RP of 2, 5, 10, 50 and 100 years and the majority of them are concentrated in the central and northern sectors of the city. During the dry season (December-April), southern CDMX has very high probability of forest fire occurrence. There is high susceptibility of landslides on the west and southwest of the city. The goals of this GIS-RA are to: 1) improve the knowledge of the potential impact of local hazards; 2) provide elements for disaster prevention, mitigation, preparedness, and response; 3) benefit decision-makers with robust risk data; 4) provide information for land-use planning; and 5) support further research to reduce the impact of disasters.
Intake of Potatoes is Associated with Higher Diet Quality, and Improved Nutrient Intake and Adequacy Among US adolescents: NHANES 2001-2018 Analysis.
Authors: Agarwal Sanjiv , Fulgoni III Victor L 
Published: June 2021

Abstract

Background: Potatoes are nutrient rich white vegetables and are most widely consumed staple food. Research on potato consumption and impact on public health is limited and inconclusive. The objective of this study was to provide updated evaluation of the cross-sectional association between potato consumption and diet quality, nutrient intake and adequacy among adolescents. Methods: Two day 24-hour diet recall data from adolescents (n=16,633; age 9-18 years) participating in National Health and Nutrition Examination Survey 2001-2018 were used. Consumers were defined as those consuming any amount of potatoes (baked or boiled potatoes, mashed potatoes and potato mixtures, fried potatoes, and potato chips) on the days of dietary recall. Usual intakes of nutrients were determined using the National Cancer Institute method and diet quality was calculated using the Healthy Eating Index-2015 (HEI-2015) scores after adjusting the data for demographic factors. Results: Intake of potatoes did not change over the last 18 years among US adolescent. Consumers of baked or boiled potatoes, mashed potatoes and potato mixtures had 4.70% higher (P<0.05) HEI-2015 total score and the score gradually increased with increasing intake quartile. HEI-2015 total scores were also higher for consumers of baked or boiled potatoes, mashed potatoes and potato mixtures, and fried potatoes (+2.01%, P<0.05) and for consumers of baked or boiled potatoes, mashed potatoes and potato mixtures, fried potatoes, and potato chips (+1.57%, P<0.05) than non-consumers. Consumers of potatoes had higher (P<0.05) intake of energy and nutrients (dietary fiber, protein, copper, magnesium, phosphorus, potassium, selenium, zinc, niacin, vitamin B6, vitamin C, vitamin K and total choline); and higher (P<0.05) nutrient adequacy for protein, copper, magnesium, phosphorus, potassium, zinc, thiamine, niacin, vitamin B6 and vitamin C, vitamin K than non-consumers. However, potato consumers also had a higher intake of sodium.Conclusion: The results show that potato consumption was associated with better diet quality, higher nutrient intake and improved nutrient adequacy. Potatoes are nutrient rich foods and encouraging their consumption may be an effective strategy for improving intakes and adequacy of key nutrients.Trial Registration: Not applicable, as this was a secondary analysis of publicly available data from the National Health and Nutrition Examination Survey (NHANES).
Laparoscopic Cholecystectomy: Review of 1430 Cases in Cure International Hospital, Kabul, Afghanistan
Authors: Farda Wais , Tani Mohammad Kamal , Manning Richard G. , Fahmi Mohammad Samim , Barai Nasratullah 
Published: June 2021

Abstract
BackgroundLaparoscopic Cholecystectomy (LC) is the gold standard for the treatment of cholelithiasis in most countries of the world. The objective of this study was to evaluate the outcomes of LC in the surgery department of Cure International Hospital, Kabul, Afghanistan. MethodsA retrospective study was conducted on 1430 LC cases performed by the general surgery department of Cure International hospital. Data was collected from patient files and the operation theatre registry for whom LC was performed during January 2008 through December, 2019. ResultsMean age was 45.77±13.45 years (14-90 years), with male/female ratio of 1:4.7. One third (33%) had comorbidities. Most of patients (~97%) were classified as ASA grade I and II. Of all patients, 26.8% of males and 13.2% of females had gallbladder inflammation (OR=2.203, 95%CI 1.56 – 2.61, P=0.000). Overall mean duration of anesthesia was 75±25.6 minutes. The conversion rate to OC was 4.6% (N=66), most commonly dense adhesions at Callot’s triangle (3.8%). The intraoperative complication rate was 17.5% (N=249), where bile/stone spillage was the most common indication (N=235, 16.4%). Immediate postoperative complication rate was 2.4% (N=35). Average length of stay (ALOS) after LC was 2.23±1.43 days (1-19 days).ConclusionThis study shows that elective LC can be performed safely in Afghanistan with comparable outcomes in terms of complications, conversion rates, and ALOS to other countries of the region and the world. Proper case selection and careful preoperative evaluation and management can decrease further conversion, intra- and postoperative complications.
Serpina3c Deficiency Induced Necroptosis Promotes Non-Alcoholic Steatohepatitis Through β-Catenin/Foxo1/TLR4 Signaling
Authors: Qian Linglin , Ji Jing-jing , Jiang Yu , Guo Jia-Qi , Wu Ya , Yang Ziwei , Ma Gen-Shan , Yao Yu-Yu 
Published: June 2021

Abstract
Background: Hepatocyte death and liver inﬂammation have been recognized as central characteristics of nonalcoholic steatohepatitis (NASH); however, the underlying molecular mechanism remains elusive. The aim of this study is to determine the precise role of serpina3c in the progression of NASH.Methods: Male Apoe-/-/serpina3c-/- double knockout (DKO) and Apoe-/- mice were fed a high-fat diet (HFD) for 12 weeks to induce NASH. Several markers of steatosis and inﬂammation were evaluated. In vitro cell models induced by palmitic acid (PA) treatment were used to evaluate the beneficial effect of serpina3c on necroptosis and the underlying molecular mechanism.Results: Compared with Apoe-/- mice, DKO mice exhibited a signiﬁcantly exacerbated NASH phenotype that included hepatic steatosis, inflammation, ﬁbrosis and liver damage, and increased hepatic triglyceride contents. We also indicated that the expression of the receptor-interacting protein 3 (RIP3) and phosphorylated mixed lineage kinase domain-like (MLKL) was increased in DKO mice. Our results found that serpina3c knockdown promoted necroptosis and lipid droplet formation under conditions of lipotoxicity in vitro. However, these phenomena were reversed by the overexpression of serpina3c. Mechanistically, downregulation of serpina3c expression promoted Foxo1 and β-catenin expression, and Foxo1 and β-catenin colocalized in the nucleus under conditions of lipotoxicity, consequently upregulating the expression of Toll-like receptor4 (TLR4). However, disruption of the Foxo1-β/catenin by Foxo1 and β-catenin inhibitors decreased TLR4 expression and ameliorated hepatic necroptosis in vitro.Conclusion: Serpina3c plays a protective role against the progression of NASH by inhibiting necroptosis. Serpina3c, a Wnt/β-catenin inhibitor, inhibits necroptosis via β-catenin/Foxo1 by inhibiting TLR4 expression.
One Pot and Facile Preparation of Pure ZnO and Cudoped Au-ZnO Nano-particles : Photocatalytic Properties
Authors: FKIRI Anis , Saidani Mohamed Ali , Smiri Leila Samia 
Published: June 2021

Abstract
In this present work, a new synthesis process, named hydrothermal method, of hybrid Au-ZnO nanoparticles (Au-Zn1-xCuxO; where 0<x<1%) was presented and discussed in detail. Nanocrystals of copper doped zinc oxide in the presence of diethylene glycol (DEG) as solvent were synthesized using the One Pot and Facile new proposed synthesis process. The photocatalytic activities of the synthesized nanoparticles were tested and analyzed for the degradation of methylene blue (MB) under white light illumination. The performances of the synthesized Au-ZnO nanoparticles showed a pronounced enhancement compared to either pure ZnO nanoparticles or to undoped Au-ZnO nanocomposites. Various key parameters such a photocatalyst loading, the MB concentration, and type current gas have been systematically investigated on the catalytic activities of the as-prepared.
Perioperative Myocardial Injury After Elective Neurosurgery: Incidence, Risk Factors and Effects on Mortality.
Authors: saka esra , Abdullah Taner , Canbaz Mert , DINC Tugce , Polat Ozlem , Sabanci Pulat Akin , Akıncı İbrahim Özkan , Tugrul Kamil Mehmet , ali achmet 
Published: June 2021

Abstract
BackgroundPerioperative myocardial injury is an important reason of mortality and morbidity after neurosurgery. It usually is missed due to asymptomatic character. In the present study, we investigated myocardial injury after noncardiac surgery (MINS) incidence, the risk factor for MINS and association of MINS with 30-day mortality in neurosurgery patients.MethodsPatients with cardiac risk who underwent elective neurosurgery were enrolled to the study. The patients’ demographics, comorbidities, medications used, medical history, and type of operation were recorded. The high-sensitivity cardiac troponin (hs-cTn) levels of the patients were measured 12, 24, and 48 hours after surgery. The patients were considered as MINS-positive if at least one of their postoperative hs-cTn measurement values was ≥14 ng/l. All the patients were followed up for 30 days after surgery for evaluation of their outcomes, including total mortality, mortality due to cardiovascular cause, and major cardiac events.ResultsTotal 312 patients completed the study and 64 (20.5%) of them was MINS positive. Long antiplatelet or anticoagulant drug cessation time (OR: 4.9, 95%CI: 2.1-9.4) was found the most prominent risk factor for MINS occurrence. Total mortality rate was 2.4% and 6.2% in patients MINS negative and positive respectively (p = 0.112). The mortality rate due to cardiovascular reasons (0.8% for without MINS, 4.7for with MINS, and p=0.026) and incidence of the major cardiac event (4% for without MINS, 10.9 for with MINS, and p=0.026) were significantly higher in patients with MINS.ConclusionsMINS is a common problem after neurosurgery and, high postoperative hs-cTn level is associated with mortality and morbidity.
"Qualitative Development and Content Validation of the "SPART" Model; a Focused Ethnography Study of Observable Diagnostic and Therapeutic Activities in the Emergency Medical Services Care Process"
Authors: Dercksen Bert , Struys Michel M.R.F. , Cnossen Fokie , Paans Wolter 
Published: June 2021

Abstract
BackgroundClinical reasoning is a crucial task within the Emergency Medical Services (EMS) care process. Both contextual and cognitive factors make the task susceptible to errors. Understanding the EMS care process’ structure could help identify and address issues that interfere with clinical reasoning. The EMS care process is complex and only basically described. AimIn this research, we aimed to define the different phases of the process and develop an overarching model that can help detect and correct potential error sources, improve clinical reasoning and optimize patient care.MethodsWe conducted a focused ethnography study utilizing nonparticipant video observations of real-life EMS deployments combined with thematic analysis of peer interviews.After an initial qualitative analysis of 7 video observations, we formulated a tentative conceptual model of the EMS care process. To test and refine this model, we carried out a qualitative, thematic analysis of 28 video-recorded cases. We validated the resulting model by evaluating its recognizability with a peer content analysis utilizing semi-structured interviews.ResultsBased on real-life observations, we defined and validated a model covering the distinct phases of an EMS deployment. We have introduced the acronym “SPART” to describe ten different phases: Start, Situation, Prologue, Presentation, Anamnesis, Assessment, Reasoning, Resolution, Treatment, and Transfer.ConclusionThe “SPART” model describes the EMS care process and helps to understand it. We expect it to facilitate identifying and addressing factors that influence both the care process and the clinical reasoning task embedded in this process.
Characteristics of Pores in Coals Samples Exposedto Acid Mine Drainage
Authors: Tang Hao , LUO Jin-zhi , ZHENG Li-wei , LIU Chi-yang , LI huahua , WU Guan-nan , Zeng Meng-li , Bai Xue-song 
Published: June 2021

Abstract
Acid mine drainage commonly occupies some pore space after underground coal mining, and this severely depreciates coal pillars, thereby posing a significant risk to mine stability. Considering that such depreciation is reflected in the microstructures of these pillars, in the present study, we propose a static immersion method suitable for coal seam samples immersion in the laboratory. We immersed the No. 2-2 coal seam samples from the Ningtiao Tower Coal Mine in Yulin, Shaanxi Province, in different acid mine drainage solutions and monitored the pH, oxidation–reduction potential (ORP), electrical conductivity (EC), total dissolved solids (TDS) among other water quality parameters for 300 h. After the immersion tests, samples were examined using scanning electron microscopy (SEM). The pH, ORP, EC, and TDS of the prepared acid mine drainage solutions increased significantly as the immersion time increased. Changes in water quality parameters are attributed to the absorption of hydrogen ions by insoluble clay minerals in the coal, which reduced the acidity, increased the pH value, and enhanced the electrical conductivity of the acid mine drainage solutions. SEM analysis reveals differences in the pore characteristics of (pore throat size and orientation) of the coal samples, and these are caused by erosive effects of the acid mine drainage solutions. Pores with throat sizes greater than 10 µm increased by 95% as the pH of the acid mine drainage solutions decreased, while the dominant pore orientation (60–90°) decreased to the 0–30° or increased to the 150–180° range, thereby increasing their randomness.