bluuebunny/crossref_metadata_2025_split huggingface dataset:
5, 7-Dihalo-8-quinolinol complex inhibits growth of ovarian cancer cells via the downregulation of expression of Wip1
Authors: Zhiwei Rao , Songbai Xia , Qi Han 
Published: January 2021

Purpose: To assess the cytotoxic effect of 5, 7-dihalo-8-quinolinol complex (DHQ) on ovarian cancer cells, and the mechanism of action involved.Methods: DHQ-mediated changes in cell viability were determined using MTT assay, while apoptosis was analyzed with flow cytometry. The effect of DHQ on cell migration was determined using inverted microscopy, while its effect on invasiveness was assessed with Giemsa dyeing. FACS Caliburinstrumentation was employed for analyzing the effect of DHQ on the cell cycle. The protein expressions of Wip1 and P53 were assayed by western blotting.Results: DHQ induced cytotoxicity against A2780 and OVCAR 3 cells in the concentration range of 0.25 - 12 μM (p < 0.05). In A2780 and OVCAR 3 cells, treatment with 12 μMDHQ resulted in 69.34 and 65.46 % apoptosis, respectively. The migratory potential and invasiveness of A2780 and OVCAR3 cells were significantly decreased by 12 μMDHQ, relative to untreated cells (p < 0.05). Moreover, treatment with 12 μMDHQ arrested cell cycle at G1/G0 phase in A2780 and OVCAR3 cells, but downregulated the protein expressions of Wip1 expression in A2780 and OVCAR3 cells.Conclusion: DHQ exerts cytotoxic effect on ovarian cancer cell growth via arrest of cell cycle and activation of apoptosis. Moreover, DHQ inhibits the migratory and invasive abilities of the cells. Thus, DHQ is a potential drug candidate for the management of ovarian cancer.
Keywords: 5,7-Dihalo-8-quinolinol complex, Ovarian cancer, Cytotoxicity, Apoptosis, Invasiveness, Migration, Cell cycle
Assessment of the therapeutic potential of anti-miR 24 and anti-miR 34 in cardiac diseases
Authors: Li Chunli , Li Jue , Sun Xiaojun , Huang Jin , Yao Chunyan 
Published: January 2021

Purpose: To study the therapeutic effects of anti-miR-24 and anti-miR-34 in cardiac diseasesMethods: H9c2 rat cardiomyocyte cell lines were transfected with the synthetic oligonucleotides antimiR-24 and anti-miR-34 which reduced the expressions of miR-24 and miR-34. Proliferation assay, real time-polymerase chain reaction (RT-PCR) and immunoblotting were carried out to determine the effect of the transfections on cardiomyocyte proliferation, expressions of miR-24 and miR-34, as well as expressions of the target genes, TGF-β1 and E2F3.Results: The proliferation ability of the transfected cells was decreased significantly, relative to negative control. In contrast, percentage apoptosis was higher in the negative control group than in transfected cells. The expression profiles of anti-miR-24 and anti-miR-34 were significantly reduced, when compared to negative control. These results were further confirmed using immunoblot which revealed that the expression of the target genes, TGF-β1 and E2F3, were upregulated in the transfected cells.Conclusion: These results suggest that the synthetic oligonucleotides (anti-miR-24 and anti-miR-34) might be useful in developing therapeutic drug targets for cardiac diseases by suppressing the expressions of miR-24 and miR-34.
Keywords: H9c2rat cardiomyocyte miRNA, Anti-miR-24, Anti-miR-34, Cardiac diseases
Quality evaluation based on color grading - relationship between chemical susbtances and commercial grades by machine version in Corni fructus
Authors: Cui Yongxia , Wang Lili , Duan Liangliang , Chen Suiqing 
Published: January 2021

Purpose: To evaluate the correlation between the commercial grade of Corni fructus determined by machine vision technology, and its chemical composition.
Methods: Loganin, morroniside, ursolic acid, water-soluble extractives, alcohol-soluble extractives, polysaccharides and total organic acids were quantitated in four Corni fructus grades classified by machine vision technology. The content of each component was determined and analyzed bymathematical statistics.
Results: Compared with low-grade samples, higher-grade counterparts contained elevated concentrations of alcohol-soluble extractives, water-soluble extractives, loganin and morroniside. In addition, principal component analysis revealed a correlation coefficient of -0.723 between Corni fructus grade and Holistic Scoring based on chemical composition, indicating a significant correlation (p < 0.01).
Conclusion: These findings indicated the rationality of the classification method based on machine vision, and further confirmed the notion of "quality evaluation based on color" of traditional Chinese medicine.
Keywords: Corni fructus, Commercial grade, Machine vision technology, Chemical substance
Involvement of Wnt/β-catenin pathway in the inhibition of invasion and epithelial-mesenchymal transition in ovarian cancer cells
Authors: Ekem Belikiz , Gong Wei , Han Lu , Wang Xinmei , Liu Na , Wan Li , Guo Qin 
Published: January 2021

Purpose: To investigate the effects of zerumbone on cell invasion, epithelial-mesenchymal transition (EMT) and the potential signaling pathway involved in ovarian cancer cells.Methods: Caov-3 cell proliferation was assessed using 3-(4,5)-dimethylthiahiazo (-z-y1)-3,5-diphenytetrazoliumromide (MTT) assay. Wound healing assay was used to determine Caov-3 cell migration while cell invasion was evaluated using Transwell assay. Protein expression was determinedby western blot.Results: Cell viability was reduced by 5, 10, 20, and 50 μM zerumbone (p < 0.05) in a concentrationdependent manner while cell migration and invasion were inhibited by 10 and 20 μM zerumbone (p < 0.05). Protein expression levels of E-cadherin and cytoplasm β-catenin were upregulated by zerumbone (p < 0.05) in a concentration-dependent manner. On the other hand, protein expression levels of Ncadherin, vimentin, ZEB1, nuclear β-catenin, and c-Myc were suppressed by zerumbone (p < 0.05) also in a concentration-dependent manner.Conclusion: The results demonstrate that zerumbone inhibits cell proliferation, migration and invasion, but represses the EMT process via inactivation of Wnt/β-catenin signaling pathway.
Keywords: Zerumbone, Ovarian cancer, Wnt/β-catenin pathway, Epithelial-mesenchymal transition
2-Amino-nicotinamide induces apoptosis of prostate cancer cells via inhibition of PI3K/AKT and phosphorylation of STA3/JAK2
Authors: Ying Junhui , Zhou Changchun , Jin Yili , Lu Daqiao , Xiong Bing , Wei Jiahui 
Published: January 2021

Purpose: To study the cytotoxicity of 2-amino-nicotinamide against prostate cancer (PCa) cells, and the underlying molecular mechanism.Methods: The effect of 2-amino-nicotinamide on cell viability and apoptosis was determined by 3-(4,5- dimethylthiazol-2-yl)-2,5-diphenyltetrazolium (MTT) and flow cytometry, respectively, while its effect on cellular production of fluorescent-oxidized product from DCFH-DA was measured using flow cytometry. Apoptosis-related protein expressions were evaluated by western blot assay.Results: 2-Amino-nicotinamide produced cytotoxicity against MCF-7, SGC7901, PCa 22Rv1 and LNCaP cancer cell lines (p < 0.05). Mechanistic data revealed that 2-amino-nicotinamide activated apoptosis, and enhanced cleavage of PARP and caspase-3 in PCa 22Rv1 and LNCaP cells. In PCa 22Rv1 and LNCaP cell lines, cytochrome C and Bax levels were enhanced by treatment with 2-aminonicotinamide, while Bcl-2 protein level was suppressed (p < 0.05). Activated expressions of PI3K, Akt and ERK in PCa 22Rv1 and LNCaP cells were down-regulated, while p38 expression was increased.Moreover, 2-amino-nicotinamide suppressed the activation of JAK2 and STAT3, but did not alter total JAK2 and STAT3 levels in PCa 22Rv1 and LNCaP cells (p < 0.05).Conclusion: 2-Amino-nicotinamide exerts cytotoxic effects on prostate carcinoma cells via activation of apoptosis and down-regulation of PI3K/AKT and STA3/JAK2. Thus, 2-amino nicotinamide is a potential bioactive agent for prostate cancer management.
Keywords: 2-Amino-nicotinamide, Apoptosis, Fluorescent-oxidized, Cytotoxicity
Denatonium benzoate decreases the effect of histamine in vitro and in rats
Authors: Zagorchev Plamen I. , Kokova Vesela Yu , Apostolova Elisaveta G. , Draganova-Filipova Milena N. 
Published: January 2021

Purpose: To evaluate the effect of denatonium benzoate (DB) in histamine-induced model of inflammation and the effect of the selective H1 receptor agonist (2-(2-Pyridyl) ethylamine) on rat gastric smooth muscle strips pretreated with DB.Methods: The anti-inflammatory effect of DB was evaluated in vivo on histamine-induced rat paw edema. In vitro studies on spontaneous muscle contraction were performed on smooth muscle strips isolated from rat gastric corpus.Results: The results showed a well-defined anti-inflammatory effect of DB (15 mg/kg) during the early stage of rat paw edema at the 15th (p < 0.001), 30th (p < 0.01) and 60th min (p < 0.001) compared to control. In vitro experiments indicated reduced spontaneous contractile activity of smooth muscle strips to H1 receptor agonist in the presence of DB (0.5 μM). The vascular effects of histamine are mediated by H1 receptors. Substances, which reduce the effect of histamine on the H1 receptors could influence the early stage of histamine-induced inflammation.Conclusion: The results show that the anti-inflammatory activity of DB probably is related to its antagonistic activity on histamine H1 receptors. The results would contribute to the search for new antiinflammatory drugs.
Keywords: Denatonium benzoate, Inflammation, Histamine, Muscle contraction
Oxidative degradation of some antibiotics by permanganate ion in alkaline medium: A kinetic and mechanistic approach
Authors: Fawzy Ahmed , Abdallah Metwally , Alqarni Nada 
Published: January 2021

Purpose: To investigate the kinetics of oxidative removal of two β-lactam antibiotics (A), namely, ampicillin and flucloxacillin.Methods: In this study, permanganate ion (MnO4-) was used as an oxidant in an alkaline medium at fixed ionic strength of 0.1 mol dm-3 and a temperature of 298 K utilizing a spectrophotometric technique. The obtained oxidation products were characterized using spot tests and FT-IR spectra.Results: The stoichiometry of the reactions was 1:4 (A : MnO4-). The reactions were a first order credence in [MnO4-] and fractional-first order kinetics in antibiotic and hydroxyl ion. Influence of ionic strength was successfully explored. Dependence of reaction rates on temperature was studied and the activation parameters were computed and discussed. A plausible mechanism for the oxidation reactions has been elucidated. A consistent rate-law expression was also derived.Conclusion: This study introduces a significant treatment method for antibiotic removal, thus helping to protect the environment and human health.
Keywords: Permanganate, Antibiotics, Oxidative degradation, Kinetics, Mechanism
ỨNG DỤNG MÔ HÌNH RBFNN ĐỂ CHẨN ĐOÁN SỰ CỐ TRONG HỆ THỐNG ĐIỀU HÒA KHÔNG KHÍ Ô TÔ
Authors: ANH TUẤN TRẦN ĐÌNH , QUỐC MINH PHAN 
Published: January 2021

Một mô hình chính xác đóng một vai trò thiết yếu để nâng cao độ chính xác của chiến lược phát hiện và chẩn đoán lỗi điều hòa không khí ô tô. Do đó, mô hình RBFNN được áp dụng trong nghiên cứu này để phản ảnh các tình trạng vận hành của hệ thống điều hòa không khí ô tô. Trong đó, chiến lược trong nghiên cứu này bao gồm mô hình RBFNN, phương pháp EWMA và bộ quy tắc chẩn đoán được kết hợp phát triển trong nghiên cứu này. Phương pháp phát hiện chẩn đoán (FDD) sự cố trên hệ thống điều hòa không khí ô tô được phát triển và đánh giá qua bộ dữ liệu thí nghiệm mô phỏng. Kết quả nghiên cứu cho thấy khả năng xấp xỉ của mô hình RBFNN đạt được độ chính xác cao và phương pháp đề xuất trong nghiên cứu này rất thiết thực cho việc phát hiện và chẩn đoán lỗi trong các hệ thống điều hòa không khí ô tô.
Burnout - Estudo sobre estresse crônico em profi ssionais da Psicologia
Authors: Andrade Biehl Katia , Tiellet Nunes Maria Lucia , Dias Fagan Antônio Ricardo , Andrade Biehl Luisa 
Published: January 2021

Esta investigación analizó la asociación entre variables sociodemográfi cas y las dimensiones del instrumento IBP - Inventario de Burnout en Psicólogos, y la asociación entre variables de actividad profesional y las dimensiones del instrumento IBP en un grupo de 915 sujetos, elegidos por conveniencia, prospectados en el esquema de bola de nieve en la membresía de Internet. Las características predominantes en la muestra fueron las mujeres, de 23 a 36 años, casadas y sin hijos, y entre las que tenían, con un promedio de uno o dos hijos entre 3 y 16 años, la actividad profesional predomina la clínica, exclusivamente, seguida de la clínica y la docencia, y la carga de trabajo semanal de 39 horas o más de trabajo. La mayoría de los sujetos no cursó estudios de posgrado, no realizó actividades consideradas estresantes ni se sometió a terapia. Los resultados mostraron un alto burnout (alto Agotamiento Emocional y Despersonalización, y bajo Rendimiento Profesional) para un perfi l formado por profesionales más jóvenes, solteros, sin hijos y con menos tiempo egresados, independientemente del género. En la asociación entre las variables relacionadas con el trabajo y las dimensiones del IBP, los sujetos con alto burnout ejercieron el multi-empleo, y los psicólogos clínicos que ejercieron en la docencia paralela tuvieron puntuaciones altas en la dimensión Despersonalización. En cuanto a las variables alta carga de trabajo semanal; no ser un estudiante de posgrado; y no tener terapia no favoreció un alto desgaste. Se concluye que además de los casos ya identifi cados con diagnóstico positivo para síndrome de burnout, la población investigada está en proceso de enfermarse por estrés.
Algumas considerações em torno ao imbroglio Posada: quando as imagens não ilustram os textos
Authors: Vieira Dias Nataly 
Published: January 2021

A impressionante obra gráfica do gravurista mexicano José Guadalupe Posada (1852-1913) – dentro da qual se destacam as famosas calaveras alegres e dançantes – tem chamado a atenção de estudiosos há décadas. Umas das questões mais controversas nesses estudos diz respeito às relações do artista com a política, sendo que existe uma espécie de imbroglio envolvendo, de um lado, a visão de um Posada revolucionário e precursor da Revolução Mexicana e, de outro, a imagem de um Posada reacionário e porfirista. O objetivo deste trabalho é matizar essas duas versões dicotômicas e propor um novo olhar sobre as relações da obra de Posada com o universo político de sua época partindo da análise de algumas de suas gravuras publicadas na imprensa popular mexicana, principalmente a partir do acirramento da oposição à ditadura de Porfirio Díaz até as últimas obras do artista, falecido em 1913. A análise toma como base a proposta de Guillermo Sunkel (2016 [1985]) sobre as “matrizes culturais” e as concepções de Mikhail Bakhtin (1993 [1965]) a respeito do papel subversivo do “riso popular”.
Experience of Postsocialist Transformation in the Slovak Republic
Authors: Tkachenko I. 
Published: January 2021

In the article the experience of post-socialist transformation in the Slovak Republic after the Velvet Revolution and gaining of independence after the collapse of Czechoslovakia in 1993 was examined. The process of reforming of the society and state, the transition from the communist to the western model of economy, the role of public sector on the path to EU and NATO accession were investigated. It was defined that the reforming of authorities, society and the state, which had already begun in the common state of Czechs and Slovaks under the leadership of V. Havel in 1990-1992, had a great value for the formation of independent Slovakia, because, despite some differences, the European destination became a key point which determined the process of accelerated modernization and the transition from planned socialism to a free market relation. It was determined that the «peaceful divorce» with Czech Republic had become one of the most important steps on the way for successful European integration of Slovakia.
The key role of civil society in the transforming of Slovak state and overcoming the negative consequences of the dominance of communist ideology on the path of European and Euro Atlantic integration was represented. The public sector has become a driving force that guided Slovak society towards accelerated modernization in 1998-2006. It was shown the process of struggle of the European choice supporters with pro-Russian policy of V. Meciar, whose period should be generally considered as a «Slovak kuchmism». The final victory of pro-European forces in the 1998 elections led to the acceleration of modernization processes in Slovakia, implementation of required changes in society and state managing necessary for EU and NATO accession.
Keberdosaan Manusia Menurut Alkitab
Authors: Manafe Yanjumseby Yeverson 
Published: January 2021

ABSTRAK Setelah kejatuhan manusia ke dalam dosa sebagaimana diuraikan dalam kitab Kejadian 3:1-24, membuat semua manusia keturunan Adam dan hawa hidup dalam dosa. Kendatipun demikian masih ada banyak orang Kristen yang belum dapat memahami dengan pasti adanya dosa di dalam hidup mereka. Jika setiap orang kristen tidak tahu bahwa ada dosa dalam dirinya maka akan sulit sekali untuk menanganinya. Oleh sebab itu, setiap orang Kristen harus mengetahui dan menyadari hakikat dari manusia berdosa serta mau melepaskan diri dari belenggu dosa. Dan hal ini akan terwujud di dalam dan melalui Roh Kudus. Berkenaan dengan keberdosaan manusia maka dalam tulisan ini akan dibahas berturut-turut mengenai keberdosaan manusia, yaitu: definisi dosa, istilah-istilah dosa dalam Alkitab, asal dosa, aspek-aspek dosa, akibat dosa dan jalan keluar dari dosa.

ABSTRACT
After the fall of man into sin as described in Genesis 3: 1-24, made all humans descend from Adam and Eve live in sin. Nevertheless there are still many Christians who have not been able to understand with certainty the existence of sin in their lives. If every Christian does not know that there is sin in him it will be very difficult to handle it. Therefore, every Christian must know and realize the nature of sinful humans and want to break away from the bondage of sin. And this will be realized in and through the Holy Spirit. With regard to human sinfulness, this paper will be discussed in succession regarding human sinfulness, namely: the definition of sin, the terms of sin in the Bible, the origin of sin, aspects of sin, the consequences of sin and the way out of sin.
Peranan Sekolah Minggu Dalam Pertumbuhan Gereja
Authors: Pattinama Yenny Anita 
Published: January 2021

ABSTRAK Pelayanan Sekolah Minggu merupakan salah satu faktor yang cukup potensif dalam proses pertumbuhan Gereja. Lebih jauh lagi Sekolah Minggu merupakan wadah yang di pakai untuk mengajar anak-anak sesuai dengan pertumbuhan usia mereka. Pelayanan Sekolah Minggu membawa pengaruh yang positif terhadap pertumbuhan Gereja, karena anak-anak Sekolah Minggu akan menjadi generasi penerus Gereja yang melanjutkan tugas dan tanggung jawab Gereja sebagai saksi Kristus di tengah-tengah dunia yang penuh dengan tantangan ini. Tantangan yang akan di hadapi oleh Gereja pada masa yang akan datang semakin banyak di karenakan perubahan zaman dunia modern yang sudah tidak lagi mementingkan ajaran agama.

ABSTRACT
Sunday School service is one of the factors that is quite potential in the process of Church growth. Furthermore, Sunday School is a place that is used to teach children according to their age growth. Sunday School services have a positive influence on the growth of the Church, because Sunday School children will become the next generation of the Church who continue the Church's duties and responsibilities as witnesses of Christ in the midst of this challenging world. The challenges that will be faced by the Church in the future will be more and more due to the changes in the modern world era that are no longer concerned with religious teachings.
Manajemen Mutu Dan Pendidikan Mutu Melalui Standar Penjaminan Mutu (SPMI) Di STT Ebenhaezer
Authors: Kaseke Fanny Y. M 
Published: January 2021

ABSTRAK Pendidikan adalah faktor yang sangat penting bagi kemajuan Bangsa dan Negara. Maka tidaklah heran jikalau manajemen mutu pendidikan adalah unsur penting yang diperhatikan oleh pemerintah untuk menghasilkan kualitas pendidikan yang baik dan unggul. Oleh sebab itu, sangat penting jikalau institusi pendidikan memperhatikan manajemen mutu melalui standart penjaminnan mutu (SPMI). Berdasarkan hal di atas, maka penelitian ini dilakukan untuk mengulas sistem SPMI yang dilakukan di STT Ebenhaezer. Dengan tujuan menghasilkan standart mutu pendidikan yang baik dan unggul seperti yang diharapkan. Maka ada beberpa hal yang dilakukan untuk mencapai hal yang dimaksud. Membangun komunikasi yang baik di semua lini kerja di STTE, secara bertahap meningkatkan alokasi anggaran ideal untuk penjaminan mutu sesuai ketentuan Kemendiknas Dikti tahun 2009, membuat panduan kriteria keberhasilan SPMI, meningkatkan kinerja staf pengelola LP2M dan penjaminan mutu di lingkungan STTE melalui penghargaan dan sanksi, mendorong terbangunnya budaya mutu yang konsisten dan berkelanjutan.

ABSTRACT
The second abstract is written in English. Abstract length maximum 250 words. At least the abstract should include the background of the study, research methods, research results and recommendations. The abstract should include two to five keywords, and the format of writing follows the template.
IMPLEMENTASI QANUN LKS MELALUI SINERGI PERBANKAN DALAM SATU KEPEMILIKAN
Authors: M. Aditya Ananda  
Published: January 2021

Abstrak
Lahirnya qanun lembaga keuangan syariah berimplikasi lembaga keuangan syariah yang beroperasi di Aceh untuk mengkonversi kegiatan usahanya dari konvensional menjadi kegiatan usaha syariah. Dalam merespon qanun ini, bank syariah melakukan sinergi perbankan dalam satu kepemilikan dalam bentuk layanan syariah bank umum (LSBU). Diantara bank umum yang membuka layanan syariah bank umum ialah PT Bank Rakyat Indonesia, PT Bank Negara Indonesia, dan PT Bank Mandiri yang telah memiliki anak usaha dalam bentuk bank umum syariah. Layanan syariah Bank Umum ini berlandaskan Peraturan Otoritas Jasa Keuangan no. 28 /POJK.03/2019 tentang Sinergi Perbankan dalam satu kepemilikan untuk pengembangan Perbankan Syariah.
Kata Kunci: LSBU, Qanun LKS, Sinergi Perbankan

Abstract
The birth of Islamic financial institutions qanun has implications for Islamic financial institutions operating in Aceh to convert their business activities from conventional to sharia business activities. In response to this qanun, sharia banks synergized banks in one ownership in the form of sharia bank commercial services (LSBU). Among the commercial banks that open sharia services are PT Bank Rakyat Indonesia, PT Bank Negara Indonesia, and PT Bank Mandiri, which already have subsidiaries in the form of sharia commercial banks. This Sharia Commercial Bank service is based on the Financial Services Authority Regulation no. 28 /POJK.03/2019 concerning Banking Synergy in one ownership for the development of Islamic Banking.
Keywords: LSBU, Qanun LKS, Banking Synergy