bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effects of Adaptive Cardiac Resynchronization Therapy With Left-Bundle-Branch Area Pacing and Coronary Sinus Pacing
Authors: Feng Xiang-Fei , Chen Ren-Hua , Zhang Rui , Yu Yi-Chi , Liu Bo , Lu Qiu-Fen , Li Yi-Gang 
Published: September 2021

Abstract
Adaptive cardiac resynchronization therapy (aCRT) is associated with improved clinical outcomes. Left bundle branch area pacing (LBBAP) has shown encouraging results as an alternative option for CRT. In this study, we observed the clinical and echocardiographic outcome of LBB-optimized aCRT in combination with synchronized LV pacing (LOT-aCRT) in heart failure patients with reduced ejection fraction and LBBB. Heart failure patients with preserved AV conduction and LBBB morphology, who underwent aCRT from February 1, 2019, to September 30, 2020 were included. The eligible patients with or without LBBAP were divided into LOT-aCRT group or BV-CRT group. In LOT-aCRT group, the CS lead was connected to the pace-sensing portion of the RV port, and the LBBAP lead was connected to the LV port. Seventeen patients were enrolled in this study (8 cases in LOT-aCRT group, 9 cases in BV-CRT group). Patients were matched for ischemic cardiomyopathy (ICM) at baseline (5 cases vs. 4 cases). QRS duration (QRSd) via BVP was narrowed from 158.0 ± 13.0 ms at baseline to 132.0 ± 4.5 ms in LOT-aCRT group (P=0.019), and further narrowed to 123.0 ± 5.7 ms (P < 0.01) via LBBAP. However, LOT-aCRT resulted in further reduction of the QRSd (121.0 ± 3.8 ms), but no statistical significance (P > 0.05). In BV-CRT group, BVP resulted in significant reduction of the QRSd from 176.7 ±19.7 ms at baseline to 143.3 ±8.2 ms (P=0.011). However, compared with LOT-aCRT, BVP has no any advantage in reducing QRSd (P > 0.05). During follow-up, patients in LOT-aCRT group showed significant improvement in LVEF and NT-proBNP levels (P < 0.01), while patients in BV-CRT group showed non-significant changes in these parameters (P >0.05). The study demonstrates that LOT-aCRT is clinically feasible in patients with systolic HF and LBBB. LOT-aCRT was associated with significant narrowing of the QRSd and improvement in LV function, especially in patients with ICM.
Purification, Characterization of an Entomopathogenic Fungal Lectin from Purpureocillium Lilacinum, and its Involvement in Pathogenesis Leading to Mycotic Keratitis
Authors: Jagadeesh Narasimhappagari , Kulkarni Supreeth , Chachadi Vishwanath B , Roy Sanhita , Inamdar Shashikala 
Published: September 2021

Abstract
A lectin PCL, from Purpureocillium lilacinum a saprophytic, filamentous fungus was purified from the crude extract of the mycelia using 70% ammonium sulphate precipitation followed by affinity chromatography on mucin-Sepharose 4 B column. PCL is a monomer with an apparent molecular mass of 18.5 kDa as revealed by SDS-PAGE under both reducing and non reducing conditions. PCL is a blood group non specific lectin and has highest affinity towards Chitin, Mucin, asialo mucin, Fetuin with a MIC of 0.15µg/mL and also recognizes L-fucose, galactose, lactose, N-acetly galactosamine, Hyaluronic acid. PCL is stable up to 60 ºC and within the pH range 4–8. To understand its role in pathogenesis, effect of PCL was evaluated on Human Corneal Epithelial Cells (HCECs). PCL showed strong glycan mediated binding to HCECsand PCL showed proinflammatory response at lower concentrations by stimulating secretion of IL-6, 8. In contrast PCL at higher concentrations revealed opposite effect of HCECs growth inhibition. All these results collectively support the involvement of PCL in mediating host pathogen interactions possibly leading to pathogenesis. In addition, considering the entomopathogenic effect of Purpureocillium lilacinum, PCL may be attributed for this beneficiary effect, which needs to be explored.
Intestinal Dysbacteriosis-Propelled T Helper 17 Cells Activation Mediate the Perioperative Neurocognitive Disorder Induced by Anesthesia/Surgery in Aged Rats
Authors: Sun Jie , Wen Y.Z. , Feng S.W. , Dai H.Y. , Mao M. , Zhou Z.H. , Li B. , Wang C.R. , Cai X.C. , Li S.M. , Yang J.J. , Ren Q. 
Published: September 2021

Abstract
Background: Perioperative neurocognitive disorders (PND) occur frequently in elderly patients after surgery, but the mechanism of PND is not very clear at present. It is reported that anesthesia/surgery could cause intestinal flora imbalance and induce neurocognitive impairment. However, the effect of intestinal flora on PND is poorly understood. We previously found that peripheral interleukin-17A (IL17A) destroyed the blood-brain barrier (BBB), leading to central inflammation and neurocognitive impairment. The small intestine is the main place where Th17 cells are produced. Therefore, we hypothesized that Th17 cells and IL-17 may be an important bridge for intestinal microbes to cause neuroinflammation. Methods: Exploratory laparotomy was performed to establish PND model under sevoflurane anesthesia. 16S rRNA high-throughput sequencing was used to detect the changes of intestinal flora. To explore the relationship between intestinal flora and PND, compound antibiotics were used to eliminate intestinal flora before anesthesia/surgery, and behavior tests, such as open field, Y maze, and fear conditioning tests were applied to detect the changes of memory ability and which was compared with the rats that did not receive compound antibiotics. The number of Th17 cells and Foxp3 cells was detected by flow cytometry in the Peyer's patches (PP), mesenteric lymph nodes (MLN), blood and brain. Hippocampus IL17, IL17RA, IL6 and IL10 were detected by Western blot. Hippocampus IL17, IL17R and IBA1 (ionized calcium binding adaptor molecule1) were detected by immunofluorescence. Results: Anesthesia/surgery caused intestinal flora imbalance and induced neurocognitive impairment, increased the number of Th17 cells in the PP, MLN, blood and brain, up-regulated the lever of IL17, IL17R and inflammatory factor production in the hippocampus. The administration of compound antibiotics before anesthesia/surgery evidently inhibited this effect, including decreased the number of Th17 cells, down-regulated the lever of IL17, IL17R and inflammatory factor production, and improved the memory function. In addition, we found that IL17R was co-labeled with IBA1 in a large amount in the hippocampus through immunofluorescence double-staining. Conclusion: Our study suggested that intestinal dysbacteriosis-propelled T helper 17 cells activation might play an important role in the pathogenesis of PND.
WITHDRAWN: Microsatellite Marker Based DNA Fingerprinting of Cotton (Gossypium Spp.) Hybrids and Their Parents
Authors: HB Santosh , Bargat Ashwini , Venoor Santhy , K.P. Raghavendra , R. Kranthi K. , V.N. Waghmare 
Published: September 2021

Abstract
Cotton production in India by vast majority comes from cotton hybrids whose genetic purity is of great significance in seed production chain and trade. Therefore, there is need to develop a rapid, reliable and reproducible technique to assess the genetic purity of cotton hybrids as traditional, morphological traits-based ‘Grow-Out Test’ is resource intensive, time consuming, tedious and not an infallible procedure. In this regard, a study was planned to understand the genetic diversity among the hybrids and their parents, and also to identify SSR markers for confirmation of genetic purity or hybridity. One intra-arboreum hybrid, CICR2 (DS 5 GMS × LD 327 Sel.), four intra-hirsutum hybrids viz., CSHH198 (CSH 19 × CSH 8), CSHH238 (SH 2379 9Y × PIL 8 Sel.), CSHH243 (CSH 2013 × CSH 43), CSHH1862 (GMS 16A × CB 33) and one hirsutum × barbadense hybrid, Phule 388 (RHC-006 × RHCb-001) along with their respective parental lines were selected for molecular characterization. Of the total 215 SSR markers surveyed, 60 markers conveyed polymorphism. The information conveyed by the polymorphic SSR markers was utilized to assess the molecular divergence among the study material. Maximum genetic dissimilarity of 0.66 was noted between Phule 388 and LD 327 (Sel.), and between RHC-006 and DS 5 (GMS). Minimum genetic dissimilarity of 0.07 was observed between CSHH1862 with CB 33, followed by 0.11 between CICR2 with DS 5 (GMS). SSR markers were highly efficient in capturing both intra-species and inter-species level diversity. The clustering and factorial analysis was in congruence with the species of Gossypium. The diploid species genotypes were clustered separately and distinctly from the rest of the genotypes. All the hirsutum hybrids and their respective parents were found closely clustered. The inter-specific hybrid, Phule 388 along with its parents was found grouped closely. The genetic purity of the hybrids was confirmed using identified SSR markers [GH486, BNL1421, BNL3594, JESPR151 for G. hirsutum hybrid CSHH198; GH486, BNL2449, JESPR151, TMB0436 for G. hirsutum hybrid CSHH238; BNL2449, JESPR151, JESPR152 for G. hirsutum hybrid CSHH243; and, GH527, BNL3812, TMB1484, TMB1645, NAU1190, BNL3816 for inter-specific G. hirsutum × G. barbadense hybrid Phule 388]. The SSR markers were efficient in analysis of hybrid seed purity. The information generated in the present study about genetic diversity and genetic purity testing will greatly facilitate quality seed production of these cotton hybrids and thus, better cotton production.
“COVID Affected Us All:” The Birth and Postnatal Health Experiences of Resettled Syrian Refugee Women During COVID-19 in Canada
Authors: Cameron Emma Stirling , Ramos Howard , Aston Megan , Kuri Marwa , Jackson Lois 
Published: September 2021

Abstract
Background: Prior to COVID-19, postnatal resettled refugee women in Canada reported barriers to healthcare and low levels of social support, contributing to maternal health morbidities. The COVID-19 pandemic appears to be further exacerbating health inequities for marginalized populations—yet the experiences of resettled refugee women are not fully known. Aim: To understand Syrian refugee women’s experiences accessing postnatal healthcare services and supports during the COVID-19 pandemic. Methods: In-depth interviews were conducted with eight resettled Syrian refugee women living in Nova Scotia (Canada) who were postnatal between March and August 2020. Data analysis was informed by constructivist grounded theory. Findings: Three themes emerged: “the impacts of COVID-19 on postnatal healthcare;” “loss of informal support;” and “grief and anxiety.” Women experienced difficult healthcare interactions, including socially and physically isolated deliveries, challenges accessing in-person interpreters, and cancelled or unavailable in-home services (e.g., public health nurse and doula visits). Increased childcare responsibilities and limited informal supports due to pandemic restrictions left women feeling overwhelmed and exhausted. Stay-at-home orders resulted in some women reporting feelings of isolation and loss, as they were unable to share in person postnatal moments with friends and family, ultimately impacting their mental wellness. Conclusions: COVID-19 and associated public health restrictions had significant impacts on postnatal Syrian refugee women. Data presented in this study demonstrated the ways in which the pandemic environment and related restrictions amplified pre-existing barriers to care and postnatal health inequalities for resettled refugee women—particularly a lack of postnatal informal supports and systemic barriers to care.
Macrophage-Biomimetic Porous Se@SiO2 Nanocomposites For “Dual Model” Immunotherapy Against Inflammatory Osteolysis
Authors: Ding Cheng , Yang Chuang , Cheng Tao , Wang Xingyan , Wang Qiaojie , He Renke , Sang Shang , Zhu Kechao , Xu Dongdong , Wang Jiaxing , Liu Xijian , Zhang Xianlong 
Published: September 2021

Abstract
Background：Inflammatory osteolysis is a major complication of total joint replacement surgery that can cause prosthesis failure and necessitate revision surgery. Macrophages are key effector immune cells in inflammatory responses, but excessive M1-polarization of dysfunctional macrophages leads to the secretion of pro-inflammatory cytokines and severe loss of bone tissue. Here, we report the development of macrophage-biomimetic porous SiO2-coated ultrasmall Se particles (Porous Se@SiO2 nanospheres) for the management of inflammatory osteolysis. Results: Macrophage-membrane-coated porous Se@SiO2 nanospheres(M-Se@SiO2) can attenuate lipopolysaccharide (LPS)-induced inflammatory osteolysis by a dual-immunomodulatory effect. As macrophage membrane decoys, these nanoparticles reduce toxin levels and neutralize pro-inflammatory cytokines. Moreover, the release of Se can induce the polarization of macrophages toward the anti-inflammatory M2-phenotype. These effects are mediated via the inhibition of p65, p38, and extracellular signal-regulated kinase(ERK) signaling. Additionally, the immune environment created by M-Se@SiO2 reduces the inhibition of osteogenic differentiation caused by pro-inflammation cytokines, confirmed through in vitro and in vivo experiments.Conclusion: Our findings suggest that M-Se@SiO2 has an immunomodulatory role in LPS-induced inflammation and bone remodeling, which demonstrates that M-Se@SiO2 is a promising engineered nano-platform for the treatment of osteolysis arising after arthroplasty.
Using Caenorhabditis Elegans As An Environmental Indicator for Impaired Urbanized Watersheds
Authors: Gerald Carresse , Deshazo Boris , Patterson Hayden , Spence Porché 
Published: September 2021

Abstract
Background Third Fork Creek is a historically impaired urban stream that flows through the city of Durham, North Carolina. Caenorhabditis elegans (C. elegans) are non-parasitic, soil and aquatic dwelling nematodes that have been used frequently as a biological and ecotoxicity model. We hypothesize that exposure to Third Fork Creek surface water will inhibit the reproduction and chemotaxis of C. elegans. Using our ring assay model, nematodes were enticed to cross the impaired water samples to reach a bacterial food source which allowed observation of chemotaxis. The total number of nematodes found in the bacterial food source and the middle of the plate with the impaired water source was recorded for three days. Results Our findings suggest a reduction in chemotaxis and reproduction on day three in nematodes exposed to Third Fork Creek water samples when compared to the control (pvalue<0.05). These exploratory data provide meaningful insight to the quality of Third Fork Creek located near a Historically Black University. Conclusions Further studies are necessary to elucidate the concentrations of the water contaminants and implications for human health. The relevance of this study lies within the model C. elegans, that has been used in a plethora of human diseases and exposure research but can be utilized as an environmental indicator of water quality impairment.
Optimization of Controllable Deformation Zone And Die Wear of Aluminum Crown Forgings For Shock Absorber Assembly
Authors: Su Hui-Zhen , Ho Ming-Hsiu , Shih Jyun-Kai , Huang Cheng-Fu , Ku Hao-Yun , Chan Chien-Wei , Fuh Yiin-Kuen 
Published: September 2021

Abstract
In this research, numerical analysis, response surface method (RSM) and experiments are used to investigate and verify the hot forging process for manufacturing aluminum crown forgings for shock absorber assembly. First, establish the computer aided design (CAD) model of the die and the billet, and simulate it from the finite element method (FEM). Second, a new preforming die was designed with a preformed dressing of controllable deformation zone (CDZ) by the CAD software. Third, numerical simulation was combined with RSM to optimize the processing parameters with the aim of minimizing the die wear while the integrity of forgings should be prioritized preserved. According to RSM, the billet size and preformed dressing of CDZ are important factors affecting the distance between die and workpiece (C). The optimal design factor of the preforming die: billet diameter (D), billet length (L) and flash design (F) are 40 mm, 205 mm and CDZ 1, respectively. Through the results of FEM, this study describes the distribution of microscopic grain flow lines are highly related to forming, stress, strain, and temperature as well as die design such as CDZ in preformed dressing. In order to accurately verify that the parameters analyzed by the RSM, both numerical analysis and physical experiments are carried out and optimal scheme exhibit reasonable consistency.
High-performance Hydrochromic Cotton Fabric Fabricated With CoCl2/waterborne Polyurethane for Air Humidity Detection
Authors: Zhang Jinju , Ge Fangqing , Tan Jialing , Yin Yunjie , Wang Chaoxia 
Published: September 2021

Abstract
Hydrochromic materials has been a novel topic in the research of stimuli-responsive sensors and anticounterfeiting encryption. A high-performance hydrochromic cotton fabric is fabricated with CoCl2/waterborne polyurethane via hot-press method. Moisture sensing metal salt cobalt chloride is introduced into waterborne polyurethane via physical doping to induce hydrochromic phenomenon. The hydrochromic cotton fabric possess superior fastness due to the hydrogen bonds formed by hydroxyl group on cotton fabric and carbonyl group, urethane bond, ether bond in waterborne polyurethane. Meanwhile, the hydroxyl group on cotton fabric and waterborne polyurethane endow hydrochromic cotton fabric with excellent hydrophilicity and high humidity sensitivity. Air humidity could be easily detected by hydrochromic cotton fabric. The absorption of humidity in rainy days led to pink color, resulting in a blue shift at 512 nm. In contrast, hydrochromic cotton fabric turn to blue color below RH 59% in sunny days, leading to a red shift for up to 674 nm. Moreover, the hydrochromic cotton fabric exhibited eminent stability and cyclicity in response to either dry state or wet condition. Based on these excellent properties, hydrochromic cotton fabric fabricated with CoCl2/waterborne polyurethane are promising as smart sensors with potential application in sensitive RH detector, anti-counterfeiting technology and decorative coatings.
COL5A3 is a prognostic biomarker and correlated with immune infiltrates in pancreatic cancer
Authors: Li Yongjie , Zeng Min , Wang Ting , Jiang Feng 
Published: September 2021

Abstract
Background

Pancreatic cancer is a malignant tumor of digestive system with high fatality rate, and its prognosis is very poor. Type Ⅴ collagen α3 (COL5A3) is highly expressed in a variety of tumor tissues, but its prognostic value and immune infiltration in pancreatic cancer are still unclear. Therefore, we evaluated the prognostic role of COL5A3 in pancreatic cancer and its correlation with immune infiltration.
Methods

The transcriptional expression profiles of COL5A3 in pancreatic cancer and normal tissues were downloaded from the Cancer Genome Atlas (TCGA). In the GEO (Gene expression omnibus) dataset (GSE16515), we compared the expression of COL5A3 in normal and tumor tissues. The expression of COL5A3 protein was evaluated by the human protein atlas (THPA). The effect of COL5A3 on survival was evaluated by Kaplan-Meier method. Receiver operating characteristic (ROC) curve was used to distinguish pancreatic cancer from paracancerous normal tissues. Protein-protein interaction (PPI) network was constructed by the STRING. Use the "ClusterProfiler" package for functional enrichment analysis. Tumor immune estimation resource (TIMER) and tumor-immune system interaction database (TISIDB) were used to determine the relationship between COL5A3mRNA expression and immune infiltration.
Results

Compared with normal tissues, COL5A3 is highly expressed in pancreatic cancer tissues. The high expression of COL5A3 is related to the poor clinicopathological features and poor prognosis of pancreatic cancer. Kaplan-Meier survival analysis showed that the prognosis of pancreatic cancer patients with high expression of COL5A3 was worse than that of patients with low expression of COL5A3. ROC curve shows that COL5A3 has high sensitivity and specificity in the diagnosis of pancreatic cancer. Correlation analysis showed that the expression of COL5A3mRNA was related to immune cell infiltration.
Conclusion

This study reveals for the first time that COL5A3 may be a new prognostic biomarker related to immune infiltration and provide a new target for the diagnosis and treatment of pancreatic cancer.
Association between Quantitative Classification of Renal Surface Nodularity and Early Renal Injury in Patients with Arterial Hypertension
Authors: Zhou Jun , Ding Jiule , Chen Jie , Pan Liang , Zha Tingting , Wu Qiong , Liu Qi , Xiang Dehui , Xing Wei 
Published: September 2021

Abstract
Objective To explore the association between quantitative classification of renal surface nodularity (qRSN) based on computed tomography (CT) imaging and early renal injury (ERI) in patients with aerial hypertension.Methods A total of 143 patients with a history of hypertension were retrospectively enrolled; clinical information (age, sex, hypertension grade, hypertension course, poorly controlled hypertension), laboratory tests and qRSN were collected or assessed. The subjects were divided into an ERI (n=60) or a control (CP, n=83) group according to ERI diagnosis based on the following criteria: cystatin C > 1.02 mg/L, creatinine ≤ 127 μmol/L, and urea nitrogen ≤ 8.3 mmol/L. Univariate analysis and multiple logistic regression were used to examine the association between ERI and qRSN. A receiver operating characteristic curve (ROC) was produced to compare multiple logistic regression models with or without qRSN for differing the ERI group from the control group.Results In univariate analysis, hypertension grade, poorly controlled hypertension, hypertension course, triglycerides (TG) and qRSN were related to ERI in patients with arterial hypertension (all P<0.1), with strong interrater agreement of qRSN. Multiple logistic regression analysis showed an area under the ROC curve of 0.705 in the model without qRSN and 0.789 in the model with qRSN, which was significantly different (Z=2.317, P=0.021).Conclusion CT imaging-based qRSN was associated with ERI in patients with arterial hypertension and may be an imaging biomarker of early renal injury.
The “Vitaminic-strategy” Against the Oral Bacteria S. Mutans and F. Nucleatum, Agents of Caries and Halitosis
Authors: Pietrangelo Laura , Magnifico Irene , Petronio Giulio Petronio , Cutuli Marco Alfio , Venditti Noemi , Nicolosi Daria , Perna Angelica , Guerra Germano , Marco Roberto 
Published: September 2021

Abstract
BackgroundThe oral cavity is one of the most complex human body environments. Indeed, the continuous variation of this habitat conditions reflects the high dynamism of the resident microbial community. Two key actors in the oral diseases are the bacteria Streptococcus mutans and Fusobacterium nucleatum, both implicated in the formation of oral biofilms and consequently in the generation of common pathologies such as caries and various gingival and soft tissue inflammation diseases. In addition, F. nucleatum is also implicated in the halitosis phenomenon, thanks to its demonstrated ability to produce as second metabolite the hydrogen sulphide (H2S), one of the volatile sulphur compounds (VSCs) that, with methyl mercaptan (CH3SH) and the dimethyl sulphide (CH3SCH3)24, is produced by periodontopathic anaerobic bacteria and causes the awkward bad breath in halitosis patients.MethodsIn this study, the oral preparation Vea® Oris constituted only by vitamin E and capric/caprylic acid was evaluated as a potential treatment of caries and periodontal diseases; the effect of the product at different concentrations on the growth and the ability of both strains to form biofilm was investigated. Regarding to F. nucleatum also the influence of Vea® Oris on the production of H2S was evaluated. ResultsOur in vitro results suggested that the Vea® Oris treatment could considerably reduce the growth and biofilm formation of both S. mutans and F. nucleatum. For F. nucleatum an appreciable reduction of the H2S production can be also obtained. ConclusionsOverall, this study highlighted the potential of Vea® Oris as a more “natural” adjuvant to prevent the biofilm and plaque formation and to reduce the smelly odour of halitosis.