bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Pedigree Analysis of EGFR p.V1010M Germline Mutation in a Family with a Family History of Non-Small-Cell Lung Cancer (NSCLC)
Authors: Li Na , Huang Depei , Liu Chunyi , Xiong Lan , She Xueke , Shen Xudong , Zhang Hushan , Jiang Youfan 
Published: June 2021

Abstract
Background: The causes of tumor can be divided into genetic factors and environmental factors, but previous studies have shown that genetic factors contribute less to lung cancer. EGFR is the most common driver gene in non-small-cell lung cancer (NSCLC), but most variations are found as somatic variations. In this study, we reported a pedigree of EGFR p.V1010M germline mutation for the first time, and explored the correlation between V1010M and NSCLC disease occurrence. Furthermore, the effect of the V1010M on the treatment of EGFR-TKIs was investigated through the treatment of the proband with simultaneous somatic mutation of EGFR p.L858R.Methods: The families were screened by NGS and Sanger sequencing, and the pedigree was drawn to investigate the relationship between EGFR p.V1010M and the occurrence of NSCLC disease. Schrodinger software was used to predict the structural function of mutant amino acid sequence proteins.Results: A total of 10 blood samples were collected from four generations of the family members, many of whom suffered from lung cancer. And 6 carriers of EGFR p.V1010M were detected. Pedigree analysis showed that there was still no evidence of correlation between EGFR p.V1010M and disease occurrence. Meanwhile, the proband detected the somatic mutation of EGFR p.L858R, and the response after the treatment of gifitinib was SD, which turned to PD 4 months later. Schrodinger software showed that the 1010th amino acid valine was located near the C terminal, and the variation to methionine had little effect on the structure of EGFR dimer.Conclusion: This study is the first report of a pedigree with EGFR p.V1010M germline mutation, which might be a pathogenic mutation and associated with EGFR-TKIs resistance in NSCLC.
VEDAS: An Efficient GPU Alternative for Store and Query of Large RDF Data Sets
Authors: Makpaisit Pisit , chantrapornchai chantana 
Published: June 2021

Abstract
Resource Description Framework (RDF) is commonly used as a standard for data interchange on the web. The collection of RDF data sets can form a large graph which consume time to query. It is known that modern Graphic Processing Units (GPUs) can be employed to execute parallel programs in order to speedup the running time. In this paper, we propose a novel RDF data representation along with the query processing algorithm that is suitable for GPU processing. Since the main challenges of GPU architecture are the limited memory sizes, the memory transfer latency, and the vast number of GPU cores. Our system is designed to strengthen the use of GPU cores and reduce the effect of memory transfer. We propose a representation consists of indices and column-based RDF ID data that can save GPU memory requirement. The indices and pre-upload filtering technique are then applied to reduce the data transfer between host and GPU memory. We add the index swapping process to facilitate the sort and join the data with the given variable and add the pre-upload step to reduce the size of results’ storage, and the data transfer time. The experimental results show that our representation is about 35% smaller that the traditional NT format and 40% less compared to that of gStore. The query processing time can be speedup ranging from 1.95 to 397.03 when compared with RDF3X and gStore processing time with WatDiv testsuite. It achieves speedup 578.57 and 62.97 for LUBM benchmark when compared to RDF-3X and gStore. The analysis shows the query cases which can gain benefits from our approach.
Ghana’s Nuclear Power and Its Role in Climate Change
Authors: Dawood A.M.A. , Darko Emmanuel O. , Glover Eric T. 
Published: June 2021

Abstract
The Ghana Nuclear Power Agenda is a programme laid out by the Ghana Atomic Energy Commission in collaboration with the government of Ghana to guide and facilitate the installation of Ghana’s first ever nuclear power plant. The nuclear power plant is expected to generate between 1000 and 12800 MW of electricity from its very first and final installations in a span of 20 years. Ghana's Third National Communication (TNC) Report to the UNFCCC indicates 59 million metric tons of carbon dioxide (MtCO2e) emission in 2011. Between 1991 and 2011, Greenhouse gas (GHG) emissions grew by 20% as energy intensity of the economy rose alongside with a growing demand in industry, transport and households. If nothing is done to curtail GHG emission from fossil-source power plants, the looming catastrophe of the changing climate will occur faster than we imagine. On the basis of this, advocacy for nuclear power has been intensified in Ghana. Nuclear power is not only environmentally friendly (zero-to-low carbon emission), it is efficient and sustainable source of energy. It offers current and future energy needs without burdening future generations with a broken environment. Using Monte Carlo’s model, the current study estimates a cumulative increase (35%) in CO2 emission between 2016 and 2026 without nuclear power in Ghana’s energy mix. With the inclusion of nuclear power in the country’s energy mix by 2029, the model estimates CO2 emission cut by 12.5% between 2029 and 2039. Thus, given the same period of time, the rate of emission of CO2 was found to be more than twice its reduction.
How Often Should Dead-Reckoned Animal Movement Paths be Corrected for Drift?
Authors: gunner Richard Michael , Holton Mark D , Scantlebury Mike D , Hopkins Phil , Shepard Emily LC , Fell Adam J , Garde Baptiste , Quintana Flavio , Gómez-Laich Agustina , Yoda Ken , Yamamoto Takashi , English Holly , Ferreira Sam , Govender Danny , Viljoen Pauli , Bruns Angela , Schalkwyk O. Louis van , Cole Nik C , Tatayah Vikash , Börger Luca , Redcliffe James , Bell Stephen H , Marks Nikki J , Bennett Nigel C , Tonini Mariano H , Williams Hannah J , Duarte Carlos M , Rooyen Martin C van , Bertelsen Mads F , Tambling Craig J , Wilson Rory P 
Published: June 2021

Abstract
BackgroundUnderstanding what animals do in time and space is important for a range of ecological questions, however accurate estimates of how animals use space is challenging. Within the use of animal-attached tags, radio telemetry (including the Global Positioning System (GPS)) is typically used to verify an animal’s location periodically. Straight lines are typically drawn between these ‘Verified Positions’ (VPs) so the interpolation of space-use is limited by the temporal- and spatial resolution of the system’s measurement. As such, parameters such as route-taken and distance travelled can be poorly represented when using VP systems alone. Dead-reckoning has been suggested as a technique to improve the accuracy and resolution of reconstructed movement paths, whilst maximising battery life of VP systems. This typically involves deriving travel vectors from motion sensor systems and periodically correcting path dimensions for drift with simultaneously deployed VP systems. How often paths should be corrected for drift, however, has remained unclear.Methods & ResultsHere, we review the utility of dead-reckoning across four contrasting model species using different forms of locomotion (the African lion Panthera leo, the Red-tailed tropicbird Phaethon rubricauda, the Magellanic penguin Spheniscus magellanicus, and the Imperial cormorant Leucocarbo atriceps). Simulations were performed to examine the extent of dead-reckoning error, relative to VPs, as a function of Verified Position correction (VP correction) rate and the effect of this on estimates of distance moved. Dead-reckoning error was greatest for animals travelling within air and water. We demonstrate how sources of measurement error can arise within VP-corrected dead-reckoned tracks and propose advancements to this procedure to maximise dead-reckoning accuracy.ConclusionsWe review the utility of VP-corrected dead-reckoning according to movement type and consider a range of ecological questions that would benefit from dead-reckoning, primarily concerning animal-barrier interactions and foraging strategies.
Comparative Study of the Functional Outcomes of Combined Medial-lateral Approach Arthrolysis With or Without External Fixation for Severe Elbow Stiffness
Authors: Xiao Dan , Gong Maoqi , chen Chen , Zha Yejun , Li Ting , Ji Shangwei , Hua Kehan , Sun Weitong , Jiang Xieyuan 
Published: June 2021

Abstract
Background: To evaluate and compare the functional outcomes of combined medial-lateral approach open arthrolysis with and without hinged external fixation. Methods: We retrospectively collected and analysed the clinical data of patients with severe elbow stiffness who were treated in our center from January 2018 to January 2019. All of them were treated with combined medial-lateral approach arthrolysis. There were 20 patients who had the hinged external fixation placed and 29 patients without the placement of the external fixation. Their baseline characteristics and functional outcomes were evaluated and compared. Results: The average follow-up time was 28.4±3.7 months. There were no significant differences in the ROM of the elbow, MEPS, VAS, DASH, or complications between the two groups. The operation time and cost of the patients without external fixation were significantly lower than patients with external fixation. Conclusion: Combined medial-lateral approach open elbow arthrolysis without external fixation is a safe and effective way to treat elbow stiffness. Level of Evidence: Therapeutic Level III; Retrospective Cohort Comparison; Treatment Study.
Gut Microbiota of Ostrinia Nubilalis Larvae Degrade Maize Cellulose
Authors: Li Junfeng , Chen Sifan , Tao Xuxiong , Shao Tao 
Published: June 2021

Abstract
BackgroundWhile most insects rely on gut bacteria to digest cellulose and produce sugars or fatty acids that are then available to the host, this has been disputed in Lepidopteran larvae due to their simple gut morphology and rapid digestive throughput. The European corn borer (ECB), Ostrinia nubilalis (Hübner), is a devastating pest that feeds the lignocellulose-rich tissues of maize plants. However, the potential role of ECB gut microbes in degrading maize cellulose remains unexplored. Here, we investigate the gut microbiota of ECB fed with different diets and the potential function of their gut bacteria in maize lignocellulose degradation.ResultsThe diversity and composition of gut bacterial communities varied dramatically between the ECB larva fed with artificial diets (ECB-D) and maize plants (ECB-M). Draft genomes of the bacterial isolates from ECB-D and ECB-M show that the principal degraders of cellulose mainly belonged to Firmicutes or Proteobacteria and were primarily found in the midgut. The bacterial isolates contained genes encoding various carbohydrate-active enzymes (CAZyme). Furthermore, scanning electron microscopy (SEM) revealed significant breakdown of lignocellulose in maize treated by the two bacterial isolates for nine days in vitro. Cellulose content in maize particles treated with BI-M were significantly lower than those treated with BI-D or the control (Kruskal–Wallis test: Χ2 = 6.72, df = 2; P = 0.0259). Metabolomic analyses reveal that maize particles treated by two bacterial isolates generate distinctive metabolomic profiles, with enrichment for different monosaccharides and amino acids.ConclusionThe results indicated that the diet of the host impacts the composition and the function of its gut microbiota, and that ECB exploits specific gut microbes to digest maize lignocellulose with distinctive products. Our study provides valuable microbiota resources for lignocellulose bioconversion.
Direct in Vitro Organogenesis From Sprouted Seeds of a Highly Economical and Ecological Valued Tree, Korean Pine
Authors: Liang Yan , Bai Xue , Xu Xin , Xu Hongguo , Wang Jing , Pan Peng 
Published: June 2021

Abstract
The de novo organogenesis system for Korean pine of this economically and ecologically coniferous species was success fully established using sprouted seeds as the original explants. After 30 days of incubation, 92.67% of explants produced direct shoots on Gupta and Durzan(DCR) medium containing 2 mg · L −1 kinetin (KT) in combination 0.5 mg · L −1 thidiazuron (TDZ) with a maximum of about 15 shoots per explant respectively. We also confirmed the organogenic regeneration pattern by scanning electron microscopy (SEM) observation. For shoot elongation and growth after 60 days of culture, we obtained the highest mean length of 34.99 mm from DCR basal media supplemented with 6-benzyladenine (6-BA; 0.2 mg · L −1 ), 1-Naphthaleneacetic acid (NAA; 0.1 mg · L −1 ), and activated charcoal (AC; 1 g · L −1 ). The highest rooting percentages of 20.74-21.48% were observed within two months in the 1/2 DCR medium (major elements halved) enriched with 0.05 mg·L −1 NAA and 0.5 or 1 mg · L −1 indole-3-butyric acid (IBA). Rooted plants showed a survival rate of 90.28% in perlite: peat: vermiculite = 1:1:1 after acclimatization. This protocol is a successful and efficient biotechnological approach to the micropropagation of Korean pine, and these data will be helpful to the clonal propagation and conservation of Korean pine.
The Mobility of Income-related Inequality in Food Preferences Among Chinese Adults: a Population-based Longitudinal Study
Authors: Xu Yongjian , Zhou Yiting , Zhu Siyu , Pramono Andi , Liu Liying , Zhu Liang 
Published: June 2021

Abstract
Background:This study aimed to investigate the income-related inequality of food preference and its mobility in the long run, and to quantify the determinants’ contributions of socioeconomic inequality mobility in food preference in China.Methods:The data were sourced from the China Health and Nutrition Survey (CHNS) conducted in 2006, 2009, 2011 and 2015, respectively. A study sample of 3940 adults were included for analysis. Five preselected questions were used to construct a summary food preference index. Cross-sectional and longitudinal concentration indices were used to measure the income-related inequality in food preference at a certain time and for a period, respectively. Health-related income mobility index was used to measure the gap between cross-sectional and longitudinal income-related health inequality concentration indices. Decomposition analysis was used to decompose income mobility index into its determinants.Results:The mean scores of food preference increased from 17.641 in 2006 to 18.881 in 2015. The richest people had the highest mean scores of food preference compared to other income groups in each year. Age, gender, income, education, and dietary knowledge score were positively associated with healthy food preference. The concentration indices of food preference score on income in each year were all positive, indicating that there was pro-rich inequality in food preference score with the rich having higher preference scores in each period. The income mobility index of food preference was -0.1426 after 4 periods, indicating that the degree of pro-rich inequality in food preference was larger than that at the baseline in the long run. Decomposition analysis showed that being married and living in Western China made healthy food preference more concentrated among the rich in the long run. Whereas increasing age and dietary knowledge contributed to making healthy food preference behavior less concentrated among the rich in the long run.Conclusions:The cross-sectional measure of food preference score inequality was underestimated in the long run. Our study suggests that people with decreased income could be taken as targeted population in future public intervention measures, and narrowing the regional gap between Eastern and Western China could be strengthened in future Chinese policies.
Protocol for Chinese Herbal Medicines Decoction Treatment of Angina Pectoris: a Systematic Review With Bayesian Network Meta-analysis
Authors: Li Nan , Pang Wentai , Yang Fengwen , Pang Bo , Jin Xinyao , Wang Keyi , Zheng Wenke , Zhang Junhua 
Published: June 2021

Abstract
Background Angina pectoris (AP) is the primary symptom of coronary heart disease. Evidence has shown Chinese herbal medicines can bring benefits to patients suffering from AP. There are many kinds of herbal medicines. However, the difference between them has not been systematically analysed. It is uncertain which one is better. For comparison and ranking, a systematic review and network meta-analysis is needed. Methods We will search for the following 6 electronic databases: China Biological Medicine DataBase(CBM), China National Knowledge Infrastructure(CNKI), WanFang Database, PubMed, Embase and the Cochrane Library, from inception to May 2021. Randomized controlled trials, which aim to assess the efficacy and safety of 5 kinds of Chinese herbal medicine for AP will be included. The outcomes include reduction of AP, improvement of the electrocardiogram and adverse events. The study screening and data extraction will be presented by 2 researchers. The risk of bias will be assessed according to the Cochrane handbook. A Bayesian network meta-analysis will be performed to compare the efficacy of 5 Chinese herbal medicines. Surface under the cumulative rank curve value will be calculated to rank the efficacy of each herbal medicine.DiscussionOur protocol will be expected that the results of this study will provide evidence for Clinical Practice Guideline, clinical decisions, and development of proprietary Chinese medicines.Systematic review registrationPROSPERO registration number: CRD42019146185.
A Novel Ultrasound-guided With Needle Visualization Pericardiocentesis Via Subcostal Approach
Authors: Adi Osman , Shamsudin Nurul Shaliza , Fong Chan Pei , Baherin Muhammad Faiz , Ahmad Azma Haryaty 
Published: June 2021

Abstract
Cardiac tamponade is a condition when fluids or blood fill the pericardial space resulting in compression of the heart and subsequently compromising hemodynamic status of the patient. It is a potentially fatal medical or traumatic emergency that requires rapid recognition and immediate treatment. Formerly, blind or surgical techniques were used to aspirate the pericardial fluid and they were associated with plenty of risks and complications. In the recent era, medical technology development has enabled us to perform the procedure safely with the assistance of ultrasound devices. The ultrasound-guided procedure is not only proven to be effective and safe, but it also has very minimal drawbacks and complications. In the literature, there are many ultrasound-guided pericardiocentesis procedures performed with different approaches at different regions. They include subcostal, parasternal, and para-apical approaches and phased array echocardiography transducers are typically preferred for the procedures. We report a case of cardiac tamponade presented with hemodynamic compromise. Ultrasound-guided pericardiocentesis was carried out using in-plane technique with high frequency linear ultrasound transducer at the subcostal region as lifesaving procedure. This particular technique provided full visualization of needle trajectory throughout the procedure. It was successfully completed with no complications and patient’s hemodynamic status improved post-procedure. This article highlights the novel use of in-plane method with high frequency linear transducer at subcostal area as a safe option for pericardiocentesis in patients with cardiac tamponade.
Elevating PKM2 Expression Indicates a Biomarker of Poor Prognosis in Patients With Liver Cancer
Authors: Lv Chaoxiang , Zhang Qiqi , Li Yuanguo , Li Entao , Li Fangxu , Wang Tiecheng , Zhu Dandan 
Published: June 2021

Abstract
M2 isoform of pyruvate kinase (PKM2) plays an important role in reprogramming of cell metabolism which is a hall-marker of tumorigenesis. PKM2 expression altering is closely related to cancer metabolism and tumor growth. In the present study, we analyzed the role of PKM2 expression in liver cancer in order to clarify its potential application value in the diagnosis and prognosis of liver cancer patients. In cancerous liver tissues, the PKM2 expression was significantly higher than normal tissues. High PKM2 expressing was related to patient’s age, gender, histological type, grade, stage, T classification and poor survival. Patients with Higher PKM2 expression had a shorter OS (P = 0.0013) and RFS (P = 0.027). ROC and Multivariate Cox analysis showed that high PKM2expression was a risk factor for patients’ poor prognosis. GSEA identified mitotic spindle, PI3K/Akt/mTOR signaling, notch signaling, apoptosis, G2M checkpoint and Wnt/β- Cantenin signaling were enriched with high PKM2 expression phenotype. These findings suggested PKM2 expression has potential as a predictive biomarker for the diagnosis and prognosis of patients with liver cancer.
GLP-1(9-36), a GLP-1 cleavage product, protects against oxidative stress and apoptosis through PI3K/Akt/NOS pathway in H9c2 cardiomyoblasts
Authors: Nuamnaichati Narawat , Parichatikanond Warisara , Mangmool Supachoke 
Published: June 2021

Abstract
GLP-1(7–36), a major active form of GLP-1 hormone, is rapidly cleaved by dipeptidyl peptidase-4 to generate a truncated metabolite, GLP-1(9–36) which has a low affinity for GLP-1 receptor (GLP-1R). GLP-1(7–36) has been shown to have protective effects on cardiovascular system through GLP-1R-dependent way. Nevertheless, the cardioprotective effects of GLP-1(9–36) have not fully understood. The present study investigated the effects of GLP-1(9–36), including its underlying mechanisms against oxidative stress and apoptosis in H9c2 cardiomyoblasts. Here, we reported that GLP-1(9–36) protects H9c2 cardiomyoblasts from hydrogen peroxide (H2O2)-induced oxidative stress by promoting the synthesis of antioxidant enzymes, glutathione peroxidase-1, catalase, and heme oxygenase-1. In addition, treatment with GLP-1(9–36) suppressed H2O2-induced apoptosis by attenuating caspase-3 activity and upregulating proapoptotic proteins, Bcl-2 and Bcl-xL. These protective effects of GLP-1(9–36) are attenuated by blockade of PI3K-mediated Akt phosphorylation and prevention of nitric oxide synthase (NOS)-induced NO production. Collectively, GLP-1(9–36) represents the potential therapeutic target for prevention of oxidative stress and apoptosis in the heart.