bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Framework for Navigating Requests for Nondisclosure in Pediatric Palliative Care
Authors: Brown Michelle R. , Sunnquist Madison , Schneider Lauren M. 
Published: October 2021

Background: The treatment courses of youth with complex, chronic, and/or life-threatening illnesses are often punctuated with many consequential decision points, particularly when new information related to diagnosis or prognosis is revealed. These junctures may prompt disagreements between and within families and medical teams related to the appropriate amount and type of information to share with the child. When the beliefs held by clinicians and families about information disclosure diverge, pediatric psychologists may be consulted to facilitate collaborative conversations and promote shared decision-making. Objective: Decision-making regarding disclosure is often complex and nuanced. This article sought to synthesize literature on factors that influence disclosure in order to offer a unified model through which pediatric psychologists could guide these discussions. Method: The current article reviews relevant literature through the lens of Bronfenbrenner’s (1994) Ecological Systems Framework. A detailed case vignette is used to illustrate the application of this framework to disclosure decisions. Conclusion: The framework presented in this article is offered as a guide for liaising with families and treatment teams, promoting enhanced understanding of divergent viewpoints, and facilitating collaborative conversations about the amount and type of information to share (or not to share) with the child. Implications for Impact Statement Individuals hold varied beliefs regarding the disclosure of diagnosis and/or prognosis to pediatric patients, and these differences can contribute to increased stress and conflict among family members and providers. This article proposes a framework to guide psychologists in navigating requests for nondisclosure.
Introduction to the Special Issue on Palliative Care, End-of-Life, and Bereavement: Integrating Psychology Into Pediatric Palliative Care
Authors: Thompson Amanda L. , Kentor Rachel A. 
Published: October 2021

Objective: While most pediatric palliative care (PPC) teams do not yet routinely include psychology, roles for pediatric psychologists in PPC are expanding. The goals of this Special Issue are to promote awareness of the breadth and depth of PPC, highlight the diverse and growing roles for psychologists as PPC clinicians, scientists, educators, and advocates, and showcase the latest interdisciplinary PPC research. Method: The nine articles in this collection describe key PPC concepts and research approaches, the value of the interdisciplinary team in PPC, the impact of child illness and child loss on caregivers, needs of grieving families, ethical challenges in PPC and at end-of-life, and disparities among underrepresented and historically marginalized populations. Results: The series of articles curated for this Special Issue illustrate the many contributions psychologists offer to the field of PPC. Conclusion: PPC-specific training and development of core competencies are necessary pathways to improve future integration of pediatric psychologists in pediatric care delivery, research, and advocacy. Implications for Impact Statement Psychologists are being increasingly recognized as offering unique and valuable contributions to the field of pediatric palliative care (PPC) as clinicians, researchers, educators, and advocates. This Special Issue has been curated to be helpful to trainees, PPC psychologists, and non-PPC psychologists alike, as well as our interdisciplinary PPC team members, as we strive to further establish our discipline as integral to the PPC field.
Sulfur cycling and host-virus interactions in Aquificales-dominated biofilms from Yellowstone’s hottest ecosystems
Authors: McKay Luke J , Nigro Olivia D , Dlakić Mensur , Luttrell Karen M , Rusch Douglas B , Fields Matthew W , Inskeep William P 
Published: October 2021

Abstract
Modern linkages among magmatic, geochemical, and geobiological processes provide clues about the importance of thermophiles in the origin of biogeochemical cycles. The aim of this study was to identify the primary chemoautotrophs and host–virus interactions involved in microbial colonization and biogeochemical cycling at sublacustrine, vapor-dominated vents that represent the hottest measured ecosystems in Yellowstone National Park (~140 °C). Filamentous microbial communities exposed to extreme thermal and geochemical gradients were sampled using a remotely operated vehicle and subjected to random metagenome sequencing and microscopic analyses. Sulfurihydrogenibium (phylum Aquificae) was the predominant lineage (up to 84% relative abundance) detected at vents that discharged high levels of dissolved H2, H2S, and CO2. Metabolic analyses indicated carbon fixation by Sulfurihydrogenibium spp. was powered by the oxidation of reduced sulfur and H2, which provides organic carbon for heterotrophic community members. Highly variable Sulfurihydrogenibium genomes suggested the importance of intra-population diversity under extreme environmental and viral pressures. Numerous lytic viruses (primarily unclassified taxa) were associated with diverse archaea and bacteria in the vent community. Five circular dsDNA uncultivated virus genomes (UViGs) of ~40 kbp length were linked to the Sulfurihydrogenibium metagenome-assembled genome (MAG) by CRISPR spacer matches. Four UViGs contained consistent genome architecture and formed a monophyletic cluster with the recently proposed Pyrovirus genus within the Caudovirales. Sulfurihydrogenibium spp. also contained CRISPR arrays linked to plasmid DNA with genes for a novel type IV filament system and a highly expressed β-barrel porin. A diverse suite of transcribed secretion systems was consistent with direct microscopic analyses, which revealed an extensive extracellular matrix likely critical to community structure and function. We hypothesize these attributes are fundamental to the establishment and survival of microbial communities in highly turbulent, extreme-gradient environments.
Increased locomotor activity via regulation of GABAergic signalling in foxp2 mutant zebrafish—implications for neurodevelopmental disorders
Authors: Lüffe Teresa M. , D’Orazio Andrea , Bauer Moritz , Gioga Zoi , Schoeffler Victoria , Lesch Klaus-Peter , Romanos Marcel , Drepper Carsten , Lillesaar Christina 
Published: October 2021

AbstractRecent advances in the genetics of neurodevelopmental disorders (NDDs) have identified the transcription factor FOXP2 as one of numerous risk genes, e.g. in autism spectrum disorders (ASD) and attention-deficit/hyperactivity disorder (ADHD). FOXP2 function is suggested to be involved in GABAergic signalling and numerous studies demonstrate that GABAergic function is altered in NDDs, thus disrupting the excitation/inhibition balance. Interestingly, GABAergic signalling components, including glutamate-decarboxylase 1 (Gad1) and GABA receptors, are putative transcriptional targets of FOXP2. However, the specific role of FOXP2 in the pathomechanism of NDDs remains elusive. Here we test the hypothesis that Foxp2 affects behavioural dimensions via GABAergic signalling using zebrafish as model organism. We demonstrate that foxp2 is expressed by a subset of GABAergic neurons located in brain regions involved in motor functions, including the subpallium, posterior tuberculum, thalamus and medulla oblongata. Using CRISPR/Cas9 gene-editing we generated a novel foxp2 zebrafish loss-of-function mutant that exhibits increased locomotor activity. Further, genetic and/or pharmacological disruption of Gad1 or GABA-A receptors causes increased locomotor activity, resembling the phenotype of foxp2 mutants. Application of muscimol, a GABA-A receptor agonist, rescues the hyperactive phenotype induced by the foxp2 loss-of-function. By reverse translation of the therapeutic effect on hyperactive behaviour exerted by methylphenidate, we note that application of methylphenidate evokes different responses in wildtype compared to foxp2 or gad1b loss-of-function animals. Together, our findings support the hypothesis that foxp2 regulates locomotor activity via GABAergic signalling. This provides one targetable mechanism, which may contribute to behavioural phenotypes commonly observed in NDDs.
Cannabis labelling is associated with genetic variation in terpene synthase genes
Authors: Watts Sophie , McElroy Michel , Migicovsky Zoë , Maassen Hugo , van Velzen Robin , Myles Sean 
Published: October 2021

AbstractAnalysis of over 100 Cannabis samples quantified for terpene and cannabinoid content and genotyped for over 100,000 single nucleotide polymorphisms indicated that Sativa- and Indica-labelled samples were genetically indistinct on a genome-wide scale. Instead, we found that Cannabis labelling was associated with variation in a small number of terpenes whose concentrations are controlled by genetic variation at tandem arrays of terpene synthase genes.
A six-metabolite panel as potential blood-based biomarkers for Parkinson’s disease
Authors: Klatt Stephan , Doecke James D. , Roberts Anne , Boughton Berin A. , Masters Colin L. , Horne Malcolm , Roberts Blaine R. 
Published: October 2021

AbstractCharacterisation and diagnosis of idiopathic Parkinson’s disease (iPD) is a current challenge that hampers both clinical assessment and clinical trial development with the potential inclusion of non-PD cases. Here, we used a targeted mass spectrometry approach to quantify 38 metabolites extracted from the serum of 231 individuals. This cohort is currently one of the largest metabolomic studies including iPD patients, drug-naïve iPD, healthy controls and patients with Alzheimer’s disease as a disease-specific control group. We identified six metabolites (3-hydroxykynurenine, aspartate, beta-alanine, homoserine, ornithine (Orn) and tyrosine) that are significantly altered between iPD patients and control participants. A multivariate model to predict iPD from controls had an area under the curve (AUC) of 0.905, with an accuracy of 86.2%. This panel of metabolites may serve as a potential prognostic or diagnostic assay for clinical trial prescreening, or for aiding in diagnosing pathological disease in the clinic.
Development of a new poly-ε-caprolactone with low melting point for creating a thermoset mask used in radiation therapy
Authors: Aoyama Takahiro , Uto Koichiro , Shimizu Hidetoshi , Ebara Mitsuhiro , Kitagawa Tomoki , Tachibana Hiroyuki , Suzuki Kojiro , Kodaira Takeshi 
Published: October 2021

AbstractThis study aimed to develop a poly-ε-caprolactone (PCL) material that has a low melting point while maintaining the deformation ability. The new PCL (abbreviated as 4b45/2b20) was fabricated by mixing two types of PCL with different molecular weights, numbers of branches, and physical properties. To investigate the melting point, crystallization temperature, elastic modulus, and elongation at break for 4b45/2b20 and three commercially available masks, differential scanning calorimetry and tensile tests were performed. The melting point of 4b45/2b20 was 46.0 °C, and that of the commercially available masks was approximately 56.0 °C (55.7 °C–56.5 °C). The elastic modulus at 60 °C of 4b45/2b20 was significantly lower than the commercially available masks (1.1 ± 0.3 MPa and 46.3 ± 5.4 MPa, p = 0.0357). In addition, the elongation at break of 4b45/2b20 were significantly larger than the commercially available masks (275.2 ± 25.0% and 216.0 ± 15.2%, p = 0.0347). The crystallization temperature of 4b45/2b20 (22.1 °C) was clinically acceptable and no significant difference was found in the elastic modulus at 23 °C (253.7 ± 24.3 MPa and 282.0 ± 44.3 MPa, p = 0.4). As a shape memory-based thermoset material, 4b45/2b20 has a low melting point and large deformation ability. In addition, the crystallization temperature and strength are within the clinically acceptable standards. Because masks made using the new PCL material are formed with less pressure on the face than commercially available masks, it is a promising material for making a radiotherapy mask that can reduce the burden on patients.
Precision management of pollination services to blueberry crops
Authors: Cavigliasso P. , Negri P. , Viel M. , Graziani M. M. , Challiol C. , Bello F. , Saez A. 
Published: October 2021

AbstractWhile the cultivated area of pollinator-dependent crops is increasing, pollinator availability is decreasing, leading to problems in many agroecosystems. For this reason, pollinator-dependent crop growers often rent beehives to support their pollination requirements to sustain fruit productivity. However, the efficiency of those pollination systems has not been extensively studied. Here, we compared the effect of “precision” pollination (i.e., application of pesticides coordinated with growers, audit of hives, dietary supplementation and individual distribution of hives) with conventional practices (i.e., pesticides applications without coordination with growers and no audit of hives, low maintenance of hives and hives distributed in large groups) on the mean level of pollination and fruit production and quality in blueberry crops. In nine blueberry fields, we measured bee visitation rate to flowers, fruit set, fruit firmness and fruit weight. On average, precision-pollinated plots had 70% more bee visits to flowers and produced 13% more fruits that were 12% heavier and 12% firmer than those obtained through conventional practices. These results showed that pollination efficiency could be improved if key management related to bee strength, distribution and health care are taken into account. Due to these results, we encourage growers and beekeepers to include precision pollination practices to both increase the productivity of blueberry fields and the wellbeing of honey bees within agroecosystems.
Novel scaffold based graphene oxide doped electrospun iota carrageenan/polyvinyl alcohol for wound healing and pathogen reduction: in-vitro and in-vivo study
Authors: Gouda Marwa H. , Ali Safaa M. , Othman Sarah Samir , A. Abd Al-Aziz Samia , Abu-Serie Marwa M. , Elsokary Noha A. , Elessawy Noha A. 
Published: October 2021

AbstractWound healing is a complicated multicellular process that involves several kinds of cells including macrophages, fibroblasts, endothelial cells, keratinocytes and platelets that are leading to their differentiation towards an anti-inflammatory response for producing several chemokines, cytokine and growth factors. In this study, electrospun nanofiber scaffold named (MNS) is composed of polyvinyl alcohol (PVA)/iota carrageenan (IC) and doped with partially reduced graphene oxide (prGO) that is successfully synthesized for wound healing and skin repair. The fabricated MNS was tested in case of infection and un-infection with E. coli and Staphylococcus and in both of the presence and in the absence of yeast as a natural nutritional supplement. Numerous biochemical parameters including total protein, albumin, urea and LDH, and hematological parameters were evaluated. Results revealed that the MNS was proved to be effective on most of the measured parameters and had exhibited efficient antibacterial inhibition activity. Whereas it can be used as an effective antimicrobial agent in wound healing, however, histopathological findings confirmed that the MNS caused re-epithelialization and the presence of yeast induced hair follicles growth and subsequently it may be used to hide formed head wound scar.
Transplantation of fecal microbiota from patients with inflammatory bowel disease and depression alters immune response and behavior in recipient mice
Authors: Jang Hyo-Min , Kim Jeon-Kyung , Joo Min-Kyung , Shin Yoon-Jung , Lee Chang Kyun , Kim Hyo-Jong , Kim Dong-Hyun 
Published: October 2021

AbstractGut dysbiosis is closely associated with the occurrence of inflammatory bowel disease (IBD) and psychiatric disorder. Here, to understand the difference of gut microbiota composition and physiological effect between IBD patients with (IBD/D+) or without depression (IBD/D−), we analyzed the fecal microbiota composition of patients with IBD with (/D+) or without depression (/D−) and healthy volunteers (HVs) and examined the effects of these fecal microbiota transplantations (FMTs) on the occurrence of systemic inflammation and anxiety/depression in mice. FMTs from patients with IBD/D+or IBD/D−caused IBD-like colitis in the transplanted mice: they increased the myeloperoxidase activity, IL-1β and IL-6 expression, and NF-κB+/CD11c+cell population in the colon. Transplantation of the IBD/D+patient feces (IBD/D+-F) caused IBD-like colitis more strongly than that of IBD/D−-F. FMTs from patients with IBD/D+also caused anxiety-/depression-like behaviors, increased the NF-κB+/Iba1+and lipopolysaccharide (LPS)+/Iba1+cell populations, and decreased the BDNF+/NeuN+cell population in the hippocampus. They increased LPS levels in the blood. FMTs from patients with IBD/D−caused anxiety-like, but not depression-like, behaviors. α-/β-diversities and composition of gut microbiota in IBD-F were different from those of HV feces (HV-F). TheEnterobacteriaceaeandEnterococcaceaepopulations and LPS levels were higher in the IBD-F than in the HV-F. TheEnterococcaceaepopulation was higher in IBD/D+-F vs. IBD/D−-F. However, the transplantation of HV-F into mice previously transplanted with IBD/D+-F significantly reduced depression-like behaviors, NF-κB+/Iba1+and LPS+/Iba1+cell populations in the hippocampus, LPS levels in the feces and blood, and IL-1β expression in the colon. These findings suggest that the outbreak of depression/anxiety may be dependent on the systemic inflammation with a leaky gut through the gut dysbiosis-attributable overproduction of bacterial LPS and suppression of tight junction protein expression in patients with IBD.
The effects of antithrombotic therapy on head trauma and its management
Authors: Koiso Takao , Goto Masayuki , Terakado Toshitsugu , Komatsu Yoji , Matsumaru Yuji , Ishikawa Eichi 
Published: October 2021

AbstractThe number of patients with traumatic intracranial hemorrhage (tICH) that are taking antithrombotics (ATs), antiplatelets (APs) and/or anticoagulants (ACs), has increased, but the influence of it for outcome remains unclear. This study aimed to evaluate an influence of AT for tICH. We retrospectively reviewed all patients with tICH treated between 2012 and 2019, and analyzed demographics, neurological status, clinical course, radiological findings, and outcome data. A total of 393 patients with tICH were included; 117 were on AT therapy (group A) and 276 were not (group B). Fifty-one (43.6%) and 159 (57.6%) patients in groups A and B, respectively, exhibited mRS of 0–2 at discharge (p = 0.0113). Mortality at 30 days was significantly higher in group A than in group B (25.6% vs 16.3%, p = 0.0356). Multivariate analysis revealed that higher age (OR 32.7, p < 0.0001), female gender (OR 0.56, p = 0.0285), pre-injury vitamin K antagonist (VKA; OR 0.42, p = 0.0297), and hematoma enlargement (OR 0.27, p < 0.0001) were associated with unfavorable outcome. AP and direct oral anticoagulant were not. Hematoma enlargement was significantly higher in AC-users than in non-users. Pre-injury VKA was at high risk of poor prognosis for patients with tICH. To improve outcomes, the management of VKA seems to be important.
Risk factors and outcomes for failure of biological reconstruction after resection of primary malignant bone tumors in the extremities
Authors: Wisanuyotin Taweechok , Paholpak Permsak , Sirichativapee Winai , Kosuwon Weerachai 
Published: October 2021

AbstractBiological reconstruction is widely used to reconstruct bone defects after resection of bone tumors in the extremities. This study aimed to identify risk factors for failure and to compare outcomes of the allograft, nonvascularized autograft, and recycled frozen autograft reconstruction after resection of primary malignant bone tumors in the extremities. A retrospective study was performed at a single center between January 1994 and December 2017. Ninety patients with primary malignant bone tumors of the extremities were treated with tumor resection and reconstruction using one of three bone graft methods: nonvascularized autograft (n = 27), allograft (n = 34), and recycled frozen autograft (n = 29). The median time for follow-up was 59.2 months (range 24–240.6 months). Overall failure of biological reconstruction occurred in 53 of 90 patients (58.9%). The allograft group had the highest complication rates (n = 21, 61.8%), followed by the recycled frozen autograft (n = 17, 58.6%) and nonvascularized autograft (n = 15, 55. 6%) groups. There was no statistically significant difference among these three groups (p = 0.89). The mean MSTS score was 22.6 ± 3.4 in the nonvascularized autograft group, 23.4 ± 2.6 in the allograft group, and 24.1 ± 3.3 in the recycled frozen autograft group. There was no significant difference among the groups (p = 0.24). After bivariate and multivariable analyses, patient age, sex, tumor location, graft length, methods, and type of reconstruction had no effects on the failure of biological reconstruction. Biological reconstruction using allograft, nonvascularized autograft, and recycled frozen autograft provide favorable functional outcomes despite high complication rates. This comparative study found no significant difference in functional outcomes or complication rates among the different types of reconstruction.
Use of ticagrelor and the risks of pneumonia and pneumonia-specific death in patients with non-acute coronary syndrome conditions: a population-based cohort study
Authors: Feng Qi , Tsoi Man Fung , Fei Yue , Cheung Ching Lung , Cheung Bernard M. Y. 
Published: October 2021

AbstractPrevious studies have shown that ticagrelor reduced risk of pneumonia in patients with acute coronary syndrome (ACS) compared to clopidogrel, however, its effect in patients with non-ACS cardiovascular diseases remains uncertain. The aim was to investigate the effect of ticagrelor on pneumonia and pneumonia-specific death compared to clopidogrel in non-ACS patients in Hong Kong. This was a population-based cohort study. We included consecutive patients using ticagrelor or clopidogrel admitted for non-ACS conditions in Hong Kong public hospitals from March 2012 to September 2019. Patients using both drugs were excluded. The outcomes of interest were incident pneumonia, all-cause death, and pneumonia-specific death. Multivariable survival analysis models were used to estimate the effects [hazard ratio (HR) and 95% confidence interval (CI)]. Propensity score matching, adjustment and weighting were performed as sensitivity analyses. In total, 90,154 patients were included (mean age 70.66 years, males 61.7%). The majority of them (97.2%) used clopidogrel. Ticagrelor was associated with a lower risk of incident pneumonia [0.59 (0.46–0.75)], all-cause death [0.83 (0.73–0.93)] and pneumonia-specific death [0.49 (0.36–0.67)]. Sensitivity analyses yielded similar results. Ticagrelor was associated with lower risk of all-cause death, pneumonia-specific death, and incident pneumonia in patients with non-ACS cardiovascular conditions, consistent with previous evidence in patients with ACS. This additional effect of anti-pneumonia should be considered when choosing a proper P2Y12 inhibitor for patients with high risk of pneumonia.
Equivalence between positive and negative refractive index materials in electrostatic cloaks
Authors: Li Xingcai , Wang Juan , Zhang Jinghong 
Published: October 2021

AbstractWe investigate, both theoretically and numerically, the equivalence relationship between the positive and negative refraction index dielectric materials in electrostatic invisibility cloak. We have derived an analytical formula that enables fast calculate the corresponding positive dielectric constant from the negative refraction index material. The numerical results show that the negative refraction index material can be replaced by the positive refractive index materials in the static field cloak. This offers some new viewpoints for designing new sensing systems and devices in physics, colloid science, and engineering applications.