bluuebunny/crossref_metadata_2025_split huggingface dataset:
Telemedicine applied to offshore medical emergencies: impacts on a Brazilian oil industry
Authors: Martins Douglas de Almeida , Menezes Ana Carolina Braga , da Silva Vanessa Uchoa de Assis Martins , Costa André Santana , Dinis Maria Alzira Pimenta , Viterbo Lilian Monteiro Ferrari 
Published: August 2021

Abstract

Background
The study aims to analyse the impact of the use of advanced telemedicine resources in the management of medical emergencies on offshore oil platforms of a major Brazilian operator during the period 2018–2019.

Methods
38 maritime platforms were analysed, totalling a sample of 5992 workers. Exploratory data analysis, adherence test to normal distribution (Ryan-Joiner normality test) and linear correlation test (Spearman’s rho) were used to characterize the data. 5 groups were defined, 1 offshore platform with advanced telemedicine resources (reference group) and 4 platforms without this support. Each group had data on the monthly amount of disembarkation due to health reasons, in 2018 and 2019, through common flights (1087 and 1207) and aeromedical evacuations (68 and 126), respectively. Finally, hypothesis testing was carried out for two independent samples, to compare the average landings of the reference group with the other groups.

Results
The reference group presented average landings for common flights lower than those of the other groups in 2018. In 2019, presented average landings for common flights lower than those of three groups and statistically equal to that of a group. In 2018 and 2019, the reference group presented statistically equal averages (P-value &gt; α = 0.05) of landings due to aeromedical evacuations.

Conclusions
Despite the possibility of other contributing factors, such as health management of each group and operation time of the platforms, this study allows us to conclude that, in 2018 and 2019, the group of maritime platforms with advanced telemedicine resources obtained, in general, lower average landings due to health reasons.
Paediatric head computed tomography dose values and the impact on image quality
Authors: Meneses Rafaela , Dias Bruno , Pimenta Andrea , Paulo Graciano , Santos Joana 
Published: August 2021

Abstract

Background
The purpose of this study is to analyse paediatric head Computed Tomography (CT) examination dose values, establish local Diagnostic Reference Levels (DRL), and perform objective image quality assessment per categorisation.

Methods
A total of 100 paediatric head CT examinations divided into 5 paediatric age categorisations were retrospectively selected: 0–3months, 3months to 1 year, 1 to 6 years, and more than 6 years. Computed Tomography Dose Index (CTDIvol - mGy) and Dose Lenght Product (DLP – mGy.cm), acquisition mode and CT scanner were collected per examination. Examinations with lower and higher dose values per categorisation were selected, and 10 Regions of Interest (ROI’s) were defined on supra and infra tentorial regions in order to access image quality, based on signal and noise values. Local DRLs were compare with the literature and with previous studies of this centre.

Results
The obtained DLP values were 580, 570, 700, 754 mGy.cm, for the categorisation of 0–3 months, 3 months to 1 year, 1 to 6 years, and more than 6 years, respectively. No significant differences were founded in dose values and image quality, per paediatric categorisation.

Conclusions
Despise previous local DRLs were defined using a different age categorisation, some paediatric aged categorisation revealed an increase of the dose values. These results must be related with the acquisition of a new CT scanner. Optimisation process is on-going and new protocols are being define.
Pharmacologically targeting tribbles gene expression in colorectal cancer
Authors: Yassuda Victor , De Sousa-Coelho Ana Luísa 
Published: August 2021

Abstract

Background
TRIB1, TRIB2 and TRIB3 belong to the mammalian Tribbles family of pseudokinases proteins. Several studies reported Tribbles oncogenic role in different types of cancer, including colorectal cancer (CRC). Though current CRC treatment can be curative, patients are in risk of disease recurrence, meaning novel pharmacological targets and strategies are required. Our goal was to analyze Tribbles gene expression in CRC in response to different drugs.

Methods
Tribbles transcript levels were obtained from GEO profiles database (NCBI). Gene data sets (GDS) were selected based on experimental drug treatment description. Statistical analysis was performed at GraphPadPrism.

Results
Compared to non-treated control, TRIB2 expression was ∼2-fold increased in colorectal adenocarcinoma samples from patients treated with cyclooxygenase-2 inhibitor celecoxib (GDS3384), though not statistically significant (P &lt; 0.1). TRIB1 was unaltered and data for TRIB3 was not available. By contrast, all Tribbles showed differential expression after treatment of SW620 colon cancer cells with supercritical rosemary extract in progressive increasing doses (0, 30, 60, 100 μg/mL) (P &lt; 0.01;GDS5416). While both TRIB1 and TRIB3 were moderately increased in a dose-dependent manner (∼18% and 13%, respectively), TRIB2 was maximally down-regulated by ∼15% after 60 μg/mL.

Conclusions
Although celecoxib exhibits antiproliferative effects in different cancer cell types, TRIB2 gene expression showed a trend to be induced after treatment, in contrast to several genes involved in fatty acid oxidation that were down-regulated, which could result from a compensatory mechanism based on a metabolic shift. Since TRIB1/TRIB3 and TRIB2 were oppositely modulated in response to rosemary extract, additional studies are needed to validate its specific pharmacological potential interest for CRC treatment.
Advanced melanoma, a pharmacological evaluation
Authors: Andrade Anabela , Balteiro Jorge 
Published: August 2021

Abstract

Background
Cutaneous melanoma is an aggressive cancer that occurs in melanocytes, located in the epidermis. Historically it has a high rate of morbidity and mortality, due to the resistance and toxicity of traditional therapies. Its incidence has increased annually by 4% to 8%. Until 2011 it was still considered a devastating and almost always fatal disease in a few months. Advances in therapies have significantly improved the results of most patients with advanced melanoma, especially those with a BRAFV600 mutation, which account for almost 50% of tumors. Before the recent evolution in treatment, the prognosis and overall survival were considered very bad. The introduction of new drugs has improved progression-free survival and overall survival, as well as producing faster clinical responses.

Methods
Comparison of endpoints such as progression-free survival and overall melanoma survival from the Summary of Product Characteristics (SPC) studies of each drug in the therapeutic groups under assessment used in the disease. The variables used were the Endpoints Global Survival at various times (12 months, 24 months, 36 months and the median) and Progression-Free Survival.

Results
Combined immunotherapy (Nivolumab and Ipilimumab) improves overall survival and progression-free survival, achieving better results than targeted therapy. In this, the combination of a BRAF inhibitor and a MEK inhibitor, presents better results with the combination of Encorafenib and Binimetinib.

Conclusions
Both targeted therapy and immunotherapy transform melanoma with a dismal prognosis into a life-threatening illness.
Malnutrition in elderly: relationship with depression, loneliness and quality of life
Authors: Simões Paula , Amaral Ana Paula , Rocha Clara 
Published: August 2021

Abstract

Background
Depression and loneliness can lead to malnutrition in the elderly, while the greater the satisfaction with food, the lower the levels of depression and isolation, improving the quality of life. Analyze the relationship between malnutrition and mental health of the elderly. The rapid growth of the elderly population and, at the same time, the increase in mental health problems, such as depression, which has a high prevalence in the elderly population, demand attention to the quality of life of the elderly. The rapid growth of the elderly population and, at the same time, the increase in mental health problems, such as depression, which has a high prevalence in the elderly population, demand attention to the quality of life of the elderly.

Methods
Geriatric Depression Scale, Lubben Social Network Scale, WHOQOL-OLD and Mini Nutritional Assessment.
33 elderly, mean age 82.67 years (SD = 8.49).

Results
There is a significant negative correlation (r = -0.542; P = 0.001) between the values of EDGT and MNA, the higher the values of EDGT (more depressed), the lower those of MNA (more malnourished). Adequate nutritional status is related to better ERSLT and QoL values, however the results are not significant.

Conclusions
There is a significant relationship between malnutrition and an increase in depressive symptoms and, although the results are not significant, an adequate state of nutrition can be related to less sense of loneliness and better quality of life. Nutritional education interventions are justified to improve mental health and quality of life in aging.
Suicide postvention in the oil industry: innovation in mental health and care with life in the world of work
Authors: de Paiva Adrianna Helena Tavares Lobato , Miliosi Ariana Ribeiro , Dinis Maria Alzira Pimenta , Viterbo Lilian Monteiro Ferrari 
Published: August 2021

Abstract

Background
Within the oil industry work process, risk, technology, confinement and changes in sociability are implied, variables for mental health actions, complexed in the psychosocial coping of the COVID-19 pandemic, involving workers and their families. Attempts or death by suicide, challenge mental health actions, require specialized practices within organizations. This study aims to describe an innovative initiative in occupational health in the oil industry in Brazil, addressing Suicide Postvention in the Workplace.

Methods
Using an epistemological basis of ‘working with groups’; ‘psychology in emergencies and disasters’; ‘mourning elaboration’, an attempt was made to systematize the specialized intervention in mental health within suicide assistance, workplace adapted. It relates an intervention-action in 5 occurrences during the year 2020, with the participation of an interdisciplinary health team including social workers, psychologists, physicians and nurses. Individual care, group approaches, leaders’ advice and family members’ support, was provided.

Results
An average of 20 individual consultations were carried out for each occurrence, 7 interventions with groups. In 4 of the cases, direct approaches from the interdisciplinary team with family members were necessary. The implantation allowed to structure the intervention in suicide emergencies; methodological legitimation; ensuring innovation in emergency mental health practice; prevention of contagious suicide; monitoring of critical cases; and continuity of support in mental health through internal and external network.

Conclusions
The results of the study will serve as input for the construction of a specialized intervention protocol for suicide postvention, constituting an innovation in the care and prevention of mental health problems for oil workers and their families.
The natural functional biopolymer matrix demonstrates to be a promise for safe cell therapy in Parkinson’s disease
Authors: Irioda Ana Carolina , Mogharbel Bassam Felipe , Stricker Priscila Elias Ferreira , Oliverira Nathalia Barth , da Rosa Nádia Nascimento , Perussolo Maiara Carolina , Borges Felipe Azevedo , Herculano Rondinelli Donizetti , de Oliveira Graeff Carlos Frederico , de Carvalho Katherine Athayde Teixeira 
Published: August 2021

Abstract

Background
Mesenchymal stem cells (MSCs) are undifferentiated cells and can be isolated from many tissues, including adipose tissue. MSCs neuronal differentiation ability has arisen interest in research with these cells in neurodegenerative diseases, such as Parkinson’s disease (PD). To differentiate MSCs in cells that produce dopamine that posteriorly potential to be a safe cell therapy for PD.

Methods
MSCs were isolated from adipose tissue, characterized by flow cytometry and trilineage differentiation, and cultivated seeded on natural functional biopolymer matrix (NFBX) to differentiate in neuronal precursors. Finally, a neural precursor was cultivated in the dopaminergic differentiation medium. The immunocytochemistry was performed with antibody anti-Nestin for precursor neural and antibodies anti-ß III-tubulin and hydroxylase tyrosine. Then, quantification of dopamine was made by the ELISA kit in the culture medium.

Results
The cytometric analysis of MSCs and the trilineage test to chondrocyte, osteocyte, and adipocyte demonstrated their pluripotency. Cells seeded and cultivated over NFBX have developed neurospheres, and their mechanical dissociated cells were Nestin positive. Dopaminergic differentiation was confirmed with positivity for ß-III tubulin and hydroxylase tyrosine. The dopamine concentration was very high in one sample (74.91 ng/mL). Without this sample, the media was 2.34 ± 2.13 ng/mL. The difference between dopamine concentrations was probably due to donors' metabolic characteristics.

Conclusions
The MSCs differentiated in neural precursor cells without genetic modification or growth factors, using only this NFBX. When these neural precursors were induced to differentiate, they were available to produce dopamine, demonstrating a functional neuronal differentiation.
Evaluation of vascular endothelial growth factor and nitric oxide associated with resveratrol supplementation
Authors: Caseiro Armando , Ferreira Carla , Silva Ana Margarida , Figueiredo João Paulo , Pereira Telmo 
Published: August 2021

Abstract

Background
Resveratrol (3,4',5-trihydroxystilbene) (RSV) is one of the main non-flavonoid natural polyphenol compounds. Evidence suggests that RSV has a key role in preventing a variety of pathological processes because of its benefits, including anti-aging, anti-inflammatory, and cardiovascular disease prevention. Vascular endothelial growth factor (VEGF) is responsible for vasculogenesis and angiogenesis, and its expression is influenced by RSV. Vascular nitric oxide (NO) acts to relax smooth muscle cells by preventing thrombogenic processes. It has been shown in vitro and in vivo that RSV is involved in NO metabolism. The aim of this work was evaluate the effects of regular low-dose RSV consumption by determining serum VEGF and NO levels compared to a control group.

Methods
The study involved 27 clinically healthy individuals, divided into a control group (placebo) and an intervention group, supplemented with 100mg RSV/day. The VEGF levels were determined by slot blot technique and NO levels were determined by spectrophotometry before (T0) and after 30 days (T1) of supplementation.

Results
The VEGF and NO levels slightly decrease from T0 to T1 moment in both study groups, showing a higher decrease in both parameters in the control group compared to the intervention group, but the variation was not statistically significant.

Conclusions
Daily supplementation with RSV is associated with benefits at the VEGF level as well as at the vascular level. However, further studies with a larger number of participants are needed to confirm the effects of RSV on VEGF and NO levels.
Evaluation of the force applied by the adductor muscles in a healthy student population performing an adduction task
Authors: Cruz Maria , Rasteiro Deolinda , Carvalho Filipe , Lains Jorge , Roseiro Luis 
Published: August 2021

Abstract

Background
The adduction and abduction movement (closing and open the legs) are important tasks in daily activities. The evaluation and monitor of the force exerted by the adductor muscles can be used as a rehabilitation progress indicator, particularly in people with injuries in the central nervous system or motor neurons. The development of biomechanical devices to quantify and identify patterns in this type of force with healthy people can be a contribution in this domain. This work, approved by the Ethical Committee of IPC, aims to evaluate the force applied by the adductor muscles from a healthy group of students, performing the closing legs task.

Methods
The study was carried out at Applied Biomechanics Laboratory of IPC, with a group of 32 healthy volunteers from the student community (19-26 yo), half men - half women, without lower limb injuries, 59,38% with a prevalence of sedentary lifestyle. Volunteers, sitting in a comfortable position, press a biomechanical device placed between the legs. The time-force was registered for four discrete open-leg positions.

Results
The maximum mean forces were 199N in women and 257N in men, both in the shorter range of the open-leg position. The peak of force decreases with open leg distance for both genders and was achieved between 34%–54% of the performance time ([3, 28] seconds). Moderate correlations were identified between forces applied and skeletal muscles and resting metabolism.

Conclusion
The time-force applied was identified and registered with a developed biomechanical device. Obtained values are reliable and can be a support for future investigations.
Mendelian randomization study reveals a population-specific putative causal effect of type 2 diabetes in risk of cataract
Authors: Zhang Haoyang , Xiu Xuehao , Xue Angli , Yang Yuedong , Yang Yuanhao , Zhao Huiying 
Published: August 2021

AbstractBackgroundThe epidemiological association between type 2 diabetes and cataract has been well established. However, it remains unclear whether the two diseases share a genetic basis, and if so, whether this reflects a putative causal relationship.MethodsWe used East Asian population-based genome-wide association studies (GWAS) summary statistics of type 2 diabetes (Ncase = 36 614, Ncontrol = 155 150) and cataract (Ncase = 24 622, Ncontrol = 187 831) to comprehensively investigate the shared genetics between the two diseases. We performed: (i) linkage disequilibrium score regression (LDSC) and heritability estimation from summary statistics (ρ-HESS) to estimate the genetic correlation and local genetic correlation pattern between type 2 diabetes and cataract; (ii) multiple Mendelian randomization (MR) analyses to infer the putative causality between type 2 diabetes and cataract; and (iii) summary-data-based Mendelian randomization (SMR) to identify candidate risk genes underling the putative causality. Moreover, to investigate the extent of the population-specific genetic effect size underlying the shared genetics between type 2 diabetes and cataract, we applied the same analytical pipeline to perform a comparative analysis on European population-based GWAS of type 2 diabetes (Ncase = 62 892, Ncontrol = 596 424) and cataract (Ncase = 5045, Ncontrol = 356 096).ResultsUsing East Asian population-based GWAS summary data, we observed a strong genetic correlation [rg = 0.58, 95% confidence interval (CI) = 0.33, 0.83), P-value = 5.60 × 10–6] between type 2 diabetes and cataract. Both ρ-HESS and multiple MR methods consistently showed a putative causal effect of type 2 diabetes on cataract, with estimated liability-scale MR odds ratios (ORs) at around 1.10 (95% CI = 1.06, 1.17). In contrast, no evidence supports a causal effect of cataract on type 2 diabetes. SMR analysis identified two novel genes MIR4453HG (βSMR = −0.34, 95% CI = −0.46, −0.22, P-value = 6.41 × 10–8) and KCNK17 (βSMR = −0.07, 95% CI = −0.09, −0.05, P-value = 2.49 × 10–10), whose expression levels were likely involved in the putative causality of type 2 diabetes on cataract. On the contrary, our comparative analysis on European population provided universally weak evidence on the genetic correlation and causal relationship between the two diseases.ConclusionsOur results provided robust evidence supporting a putative causal effect of type 2 diabetes on the risk of cataract in East Asians, and revealed potential genetic heterogeneity in the shared genetics underlying type 2 diabetes and cataract between East Asians and Europeans. These findings posed new paths on guiding the prevention and early-stage diagnosis of cataract in type 2 diabetes patients.
The World of Immunization: Achievements, Challenges, and Strategic Vision for the Next Decade
Authors: Lindstrand Ann , Cherian Thomas , Chang-Blanc Diana , Feikin Daniel , O’Brien Katherine L 
Published: August 2021

Abstract
Immunization is among the most cost-effective public health interventions available and is estimated to have averted at least 37 million deaths between 2000 and 2019. Since the establishment of the Expanded Programme on Immunization in 1974, global vaccination coverage increased and the coverage gap between rich and poor countries decreased. Creation of Gavi, the Vaccine Alliance, in 2000 allowed the poorest countries in the world to benefit from new, life-saving vaccines and expand the breadth of protection against an increasing number of vaccine-preventable diseases. Despite this progress, inequities in access to and uptake of vaccines persist. Opportunities to realize the full potential of vaccines are within reach but require focused, tailored and committed action by Governments and immunization stakeholders. The Immunization Agenda 2030 provides a framework for action during the next decade to attain a world where everyone, everywhere, at every age fully benefits from vaccines for good health and well-being.
Functional Antibodies and Innate Immune Responses to WRSS1, a Live Oral Shigella sonnei Vaccine Candidate, in Bangladeshi Adults and Children
Authors: Sarker Protim , Mily Akhirunnesa , Ara Anjuman , Haque Farjana , Maier Nicole , Wierzba Thomas F , Walker Richard I , Venkatesan Malabi M , Raqib Rubhana 
Published: August 2021

Abstract

Background
We demonstrated in a randomized placebo-controlled trial that WRSS1, a live oral Shigella sonnei vaccine candidate, is safe in Bangladeshi adults and children, and elicits antigen-specific antibodies. Here, we describe functional antibody and innate immune responses to WRSS1.

Methods
Adults (18–39 years) and children (5–9 years) received 3 doses of 3 × 105 or 3 × 106 colony forming units (CFU) of WRSS1 or placebo, 4 weeks apart; children additionally received 3 × 104 CFU. Blood and stool were collected at baseline and 7 days after each dose. Functional antibodies were measured using serum bactericidal antibody (SBA) assay. Cytokine/chemokine concentrations were measured in lymphocyte cultures. Host defense peptides LL-37, HBD-1, and HD-5 were analyzed in plasma and stool.

Results
Children showed increased SBA titers over baseline after the third dose of 3 × 106 CFU (P = .048). Significant increases of Th-17 and proinflammatory cytokines (TNF-α, G-CSF, MIP-1β), and reduction of anti-inflammatory and Th2 cytokines (IL-10, IL-13, GM-CSF) were observed in children. Plasma HBD-1 and LL-37 decreased in children after vaccination but were increased/unchanged in adults.

Conclusions
Functional antibodies and Th1/Th17 cytokine responses in children may serve as important indicators of immunogenicity and protective potential of WRSS1.
Clinical Trials Registration: NCT01813071.