bluuebunny/crossref_metadata_2025_split huggingface dataset:
Correlation of receptor density and mRNA expression patterns in the human cerebral cortex
Authors: Matej Murgaš , Paul Michenthaler , Bruce Reed Murray , Gregor Gryglewski , Rupert Lanzenberger 
Published: December 2021

AbstractChanges in distribution of associated molecular targets have been reported across several neuropsychiatric disorders. However, the high-resolution topology of most proteins is unknown and simultaneous in vivo measurement in multi-receptor systems is complicated. To account for the missing proteomic information, mRNA transcripts are typically used as a surrogate. Nonetheless, post-transcriptional and post-translational processes might cause the discrepancy between the final distribution of proteins and gene expression patterns. Therefore, this study aims to investigate ex vivo links between mRNA expression and corresponding receptor density in the human cerebral cortex.To this end, autoradiography data on the density of 15 different receptors in 38 brain regions were correlated with the expression patterns of 50 associated genes derived from microarray data (mA), RNA sequencing data (RNA-Seq) provided by the Allen Human Brain Atlas and predicted mRNA expression patterns (pred-mRNA). Spearman’s rank correlation was used to evaluate the possible links between proteomic data and mRNA expression patterns.Correlations between mRNA and protein density varied greatly between targets: Positive associations were found for e.g. the serotonin 1A (pred-mRNA: rs = 0.708; mA: rs = 0.601) or kainate receptor (pred-mRNA: rs = 0.655; mA: rs = 0.601; RNA-Seq: rs = 0.575), while most of the investigated target receptors showed low or negative correlations.The high variability in the correspondence of mRNA expression and receptor warrants caution when inferring the topology of molecular targets in the brain from transcriptome data. This highlights the longstanding value of molecular imaging data and the need for comprehensive proteomic data.
MALDI mass spectrometry imaging of fresh and processed food: constituents, ingredients, contaminants and additives
Authors: Kokesch-Himmelreich Julia , Wittek Oliver , Race Alan M. , Rakete Sophie , Schlicht Claus , Busch Ulrich , Römpp Andreas 
Published: December 2021

AbstractMass Spectrometry imaging (MS imaging) provides spatial information for a wide range of compound classes in different sample matrices. We used MS imaging to investigate the distribution of components in fresh and processed food, including meat, dairy and bakery products. The MS imaging workflow was optimized to cater to the specific properties and challenges of the individual samples. We successfully detected highly nonpolar and polar constituents such as beta-carotene and anthocyanins, respectively. For the first time, the distribution of a contaminant and a food additive was visualized in processed food. We detected acrylamide in German gingerbread and investigated the penetration of the preservative natamycin into cheese. For this purpose, a new data analysis tool was developed to study the penetration of analytes from uneven surfaces. Our results show that MS imaging has great potential in food analysis to provide relevant information about components’ distributions, particularly those underlying official regulations.HighlightsInvestigation of fresh and processed food by MALDI mass spectrometry imagingVisualization of different compound classes in plant and meat-based foodDevelopment of data processing tool for penetration/diffusion analysis (in food)Natamycin penetration in cheese, first visualization of food additive by MS imagingAcrylamide in gingerbread, first visualization of contaminant by MS imaging
HuluFISH non-denaturing in situ detection of genomic DNA opened by CRISPR-Cas9 Nickase and Exonuclease
Authors: Han Kang , Liu Sheng , Cheng Yongsheng 
Published: December 2021

AbstractDNA fluorescence in situ hybridization (FISH) has been widely used in diagnosis and genetic research. Traditional Bacterial artificial chromosome (BAC) or oligonucleotide based probe to detect DNA in situ is only effective when the target is relatively large, usually over 150Kb DNA fragments. And it involves heat denaturation steps to open the DNA for in situ hybridization. The heat process can affect the fine structure of nuclei. Here we reported a novel method based on Cas9 nickase and exonuclease digestion of double strand DNA and permanently mark the DNA in single strand state for FISH. With this novel design, we detected non-repetitive genomic loci as small as 2Kb.
SEX-SPECIFIC TRAJECTORIES OF REELIN-DEPENDENT MATURATION OF DEEP LAYER PREFRONTAL NEURONS
Authors: Silva Hurtado Thenzing Judá , Lassalle Olivier , Ameloot Antoine , Chavis Pascale 
Published: December 2021

ABSTRACTThroughout early adulthood, the anatomical and functional maturation of PFC circuitry continues under the influence of multiple extrinsic and intrinsic factors, most notably electrical activity, and molecular cues. We previously showed that the extracellular matrix protein reelin orchestrates the structural and functional maturation of deep layers medial PFC (mPFC) pyramidal neurons. Additionally, we reported that reelin haploinsufficiency is associated to prefrontal disruptions of long-term memory retention thereby illustrating the eminent role of reelin in cognitive maturation of the PFC. Prefrontal maturation follows a sex-specific developmental pattern, supporting the existence of sexual differences in the morphology-functional properties PFC. Here, we interrogated the role of reelin in the functional maturation of excitatory networks in the mPFC. The developmental trajectory of reelin’s expression and deep layer pyramidal neurons synaptic plasticity was tracked in the mPFC of male and female mice, from the juvenile period to adulthood. To assess the role of reelin in both sexes, wild-type and heterozygous reeler mice (HRM) were compared. The results show that the maturational profile of reelin expression in the mPFC is sex-dependent and that the developmental trajectory of long-term potentiation is different between wild-type males and females. These data demonstrate reelin’s influence on prefrontal synapses is sex and period specific.
Comparative assessment of electronic nicotine delivery systems aerosol and cigarette smoke on endothelial cell migration: the Replica Project
Authors: Caruso Massimo , Emma Rosalia , Distefano Alfio , Rust Sonja , Poulas Konstantinos , Giordano Antonio , Volarevic Vladislav , Mesiakaris Konstantinos , Boffo Silvia , Arsenijevic Aleksandar , Karanasios Georgios , Pulvirenti Roberta , Ilic Aleksandar , Canciello Angelo , Zuccarello Pietro , Ferrante Margherita , Polosa Riccardo , Li Volti Giovanni ,   the Replica Project Group
Published: December 2021

AbstractCigarette smoking is associated with impairment of repair mechanisms necessary for vascular endothelium homeostasis. Reducing the exposure to smoke toxicants may result in the mitigation of the harmful effect on the endothelium and cardiovascular disease development. Previous investigations performed by the tobacco industries evaluated in vitro the effect of electronic cigarette (e-cig) compared to cigarette smoke demonstrating a significant reduction in endothelial cell migration inhibition following e-cig aerosol exposure. In the present study, we replicated one of these studies, evaluating the effects of cigarette smoke on endothelial cell migration compared to e-cig and heated tobacco products. We used a multi-center approach (ring-study) to verify the robustness and reliability of the results obtained in the replicated study. Consistently with the original study, we observed a substantial reduction of the effects of e-cig and tobacco heated products on endothelial cell migration compared to cigarette smoke. In conclusion, our study further confirms the importance of e-cig and tobacco heated products as a possible harm reduction strategy for cardiovascular diseases development in smokers.HighlightsCigarette smoking is strictly related to impairment of vascular repair mechanismsReducing the exposure to toxicants in smoke, could reduce the harm to endotheliumENDS showed a reduced effect on endothelial cell migration compared to cigaretteThese data demonstrated the reduced toxicity of ENDS compared to cigarettes.
Megaherbivores modify forest structure and increase carbon stocks through multiple pathways
Authors: Berzaghi Fabio , Bretagnolle François , Durand-Bessart Clémentine , Blake Stephen 
Published: December 2021

AbstractMegaherbivores have pervasive ecological effects. In African rainforests, elephants can increase aboveground carbon, though the mechanisms are unclear. Here we combine a large unpublished dataset of forest elephant feeding with published browsing preferences totaling > 120,000 records covering 700 plant species, including nutritional data for 102 species. Elephants increase carbon stocks by: 1) promoting high wood density tree species via preferential browsing on leaves from low wood density species, which are more digestible; 2) dispersing seeds of trees that are relatively large and have the highest average wood density among tree guilds based on dispersal mode. Loss of forest elephants could cause a 5-12% decline in carbon stocks due to regeneration failure of elephant-dispersed trees and an increase in abundance of low wood density trees. These results show the major importance of megaherbivores in maintaining diverse, high-carbon tropical forests. Successful elephant conservation will contribute to climate mitigation at a scale of global relevance.
Reconfigurable microfluidic circuits for isolating and retrieving cells of interest
Authors: Deroy Cyril , Wheeler James H. R. , Rumianek Agata N. , Cook Peter R. , Durham William M. , Foster Kevin , Walsh Edmond J. 
Published: December 2021

AbstractMicrofluidic devices are widely used in many fields of biology, but a key limitation is that cells are typically surrounded by solid walls, making it hard to access those that exhibit a specific phenotype for further study. Here, we provide a general and flexible solution to this problem that exploits the remarkable properties of microfluidic circuits with fluid walls – transparent interfaces between culture media and an immiscible fluorocarbon that are easily pierced with pipets. We provide two proofs-of-concept in which specific cell sub-populations are isolated and recovered: i) murine macrophages chemotaxing towards complement component 5a, and ii) bacteria (Pseudomonas aeruginosa) in developing biofilms that migrate towards antibiotics. We build circuits in minutes on standard Petri dishes, add cells, pump in laminar streams so molecular diffusion creates attractant gradients, acquire time-lapse images, and isolate desired sub-populations in real-time by building fluid walls around migrating cells with an accuracy of tens of micrometres using 3D-printed adaptors that convert conventional microscopes into wall-building machines. Our method allows live cells of interest to be easily extracted from microfluidic devices for downstream analyses.
University business idea incubation and stakeholders' engagement: closing the gap between theory and practice
Authors: Secundo Giustina , Mele Gioconda , Passiante Giuseppina , Albergo Francesco 
Published: December 2021

PurposeThe paper aims to contributes on the debates about University Idea Incubation by investigating the role and the engagement of different University's stakeholders in the process of opportunity recognition in an entrepreneurship education program targeted at students with an interdisciplinary background.Design/methodology/approachThrough a longitudinal case study methodology, the Contamination Lab at University of Salento (Lecce, Italy), the learning approaches and the knowledge process to create an entrepreneurial awareness, mindset and capability in students with different educational background are presented.FindingsThe findings demonstrates the crucial role of stakeholders' engagement for business idea presentation, open innovation challenge, contamination workshop on specialized topics, enterprise projects are important vehicle for effective students' business ideas and innovative projects development in a multidisciplinary environment. The close interaction among students, academia, companies and institutions creates a favourable environment that enables opportunity identification, idea generation through a deep contamination of knowledge, skills and experiences.Research limitations/implicationsLimitations include the need to generalise the results even if this limitation is typical of the case study methodology. Other research is necessary for an in-depth analysis in deep of the other Contamination Lab in Italy and to derive the “invariance traits” of this environment according to the features of the local entrepreneurial ecosystems.Practical implicationsImplications for practices include recommendations for designing innovative programs where the interactions between University-Institutions-Industry are realized.Originality/valueA conceptual framework is proposed by defining all the entrepreneurial knowledge process and knowledge creation within the Contamination Lab, highlighting the contribution of the stakeholders in each phase and learning initiative of the program.
The administrative state in food policymaking: a fait accompli
Authors: Bancerz Margaret 
Published: December 2021

PurposeThis paper analyzes scholarly literature and the development of a nonstate food strategy in Canada, the Conference Board of Canada's Canadian Food Strategy, to explore the role of the administrative state in food policymaking.Design/methodology/approachThis research is based on an exploratory case study drawing data from 38 semistructured interviews, including elite interviews. It also draws on policy documents from the nonstate food strategy.FindingsThis paper shows that various nonstate actors, including large food industry players, identify a role for the state in food policy in two ways: as a “conductor,” playing a managing role in the food policy process, and as a “commander,” taking control of policy development and involving nonstate actors when necessary. The complex and wicked aspects of food policy require the administrative state's involvement in food policymaking, while tamer aspects of food policy may be less state-centric.Originality/valueThis paper fills gaps in studies exploring food policymaking processes as well as the administrative state's role in food policymaking in a governance era. It contributes to a better understanding of the state's role in complex and wicked policy domains.
Financial feasibility assessment of adopting active controlled atmosphere containers for exporting highly perishable fruits during COVID-19: case of Taiwanese atemoya
Authors: Huang Wen-Hung , Dy Kenneth Bicol , Chang Ching-Cheng , Hsu Shih-Hsun 
Published: December 2021

PurposeThis study deals with attenuating the risk of relying on a single export market, which was heightened by the outbreak of the COVID-19 pandemic. It focuses on Taiwanese atemoya (a fruit with short storage life) and the adoption of active controlled atmosphere (CA) containers, a new technology which lengthens storage time for other export markets. This study looks at the financial feasibility of the technology's first ever use in atemoya exports.Design/methodology/approachApart from the standard financial assessment tools—like net present value (NPV), internal rate of return (IRR), benefit-cost ratio (BCR) and payback period (PBP)—this study calibrated five different scenarios based on data gathered from relevant market agents including suppliers, exporters, customs brokers and technology developer.FindingsDue to the high profit margin and low investment cost, the use of active CA containers for long-haul exports of this highly perishable fruit is found both technically and financially feasible, despite the generally higher operational cost during the pandemic.Research limitations/implicationsThis study looked at three specific export markets: Malaysia, Dubai and Canada. Results here may lack generalizability in other markets, although it is believed that slight deviations would not invalidate the conclusions of this research because short, medium and long distances were all covered therein.Originality/valueThis paper studies the first time that active CA is used for export of atemoyas to expand existing markets.
Role of cloud computing technology in the education sector
Authors: Thavi Riddhi , Jhaveri Rujuta , Narwane Vaibhav , Gardas Bhaskar , Jafari Navimipour Nima 
Published: December 2021

Purpose
This paper aims to provide a literature review on the cloud-based platforms for the education sectors. The several aspects of cloud computing adoption in education, remote/distance learning and the application of cloud-based design and manufacturing (CBDM) have been studied and theorised.

Design/methodology/approach
A four-step methodology was adopted to analyse and categorise the papers obtained through various search engines. Out of 429 research articles, 72 papers were shortlisted for the detailed analysis.

Findings
Many factors that influence cloud computing technology adoption in the education sector have been identified in this paper. The research findings on several research items have been tabulated and discussed. Based on the theoretical research done on cloud computing for education, cloud computing for remote/distance learning and CBDM, cloud computing could enhance the educational systems in mainly developing countries and improve the scope for remote/distance learning.

Research limitations/implications
This study is limited to papers published only in the past decade from 2011 to 2020. Besides, this review was unable to include journal articles published in different languages. Nevertheless, for the effective teaching and learning process, this paper could help understand the importance and improve the process of adopting cloud computing concepts in educational universities and platforms.

Originality/value
This study is a novel one as a research review constituting cloud computing applications in education and extended for remote/distance learning and CBDM, which have not been studied in the existing knowledge base.
Teaching for creativity and entrepreneurial intentions: an empirical study
Authors: Nguyen Thuy Thu , Do Diep Ngoc 
Published: December 2021

Purpose
This study aims to investigate the structural relationships between pedagogy methods, creativity and entrepreneurial intentions. This study theorizes that work-integrated and active learning methods positively influence personal creativity, which in turn positively influences entrepreneurial intention. Moreover, entrepreneurial inspiration moderates the impact of creativity on entrepreneurial intentions.

Design/methodology/approach
A sample of 701 valid questionnaires from university students is used to investigate the proposed research model empirically. As the methodological approach, structural equation modelling with multigroup analysis is used.

Findings
The statistical results confirm the positive impacts of work-integrated and active learning methods on individual perceived creativity and of creativity on entrepreneurial intentions. Further, inspiration towards entrepreneurship moderates the link between individual creativity and entrepreneurial intentions.

Originality/value
This research fills the gap in the realm of examining the structural relationship among learning methods, creativity and entrepreneurial intentions in an emerging economy context. Specifically, this study confirms experiential teaching methods as important predictors of the development of students’ creativity capability and the moderating role of entrepreneurial inspiration in the relationship between perceived creativity and entrepreneurial intention. These results add to the academic literature on entrepreneurial intentions in emerging economies and provide new insights into how universities in emerging economies can foster the entrepreneurial intentions of their students.
Gender-based characteristics of micro, small and medium-sized enterprises in an emerging country: is this a man’s world?
Authors: Sanchez-Riofrio Angelica Maria , Lupton Nathaniel C. , Camino-Mogro Segundo , Acosta-Ávila Álvaro 
Published: December 2021

PurposeWorldwide, Ecuador is one of the countries with the most entrepreneurial activity from micro, small and medium-sized enterprises (MSMEs). However, the effect of adopting the US dollar (dollarization), over which the central bank has no control, combined with being mainly an exporter of primary products, as well as strategic currency devaluation by neighboring economies, has created a difficult situation, especially for Ecuadorian women’s MSMEs. This paper aims to study the relationship between female ownership and Ecuadorian MSMEs’ financial, economic and social outcomes.Design/methodology/approachThe authors compile a near-population panel of 617,804 firm-year observations representing an unbalanced panel of 112,917 MSMEs during the 2007–2016 sampling window. Panel (fixed effects) regression is used to test the hypotheses concerning the antecedents to firm financial performance, economic and social outcomes. Cox proportional hazards modeling is used to assess the impact of antecedents on firm survival.FindingsFirst, firms providing more social benefits (e.g. employment and higher wages) have higher survival rates. Second, female ownership is negatively related with microenterprise financial performance, but positively associated with small-enterprise financial performance. Third, female-owned enterprises tend to provide higher wages per employee for all firm sizes. Fourth, although female-owned microenterprises are less efficient, they tend to provide more for their employees and possibly communities, through the economic stimulus they provide, in terms of the size of the financial outcomes.Originality/valueThis paper shows that, although this is a “man’s world,” women are learning earlier, developing faster professionally and overcoming stereotypes to focus on activities that generate both economic performance and social outcomes. Governmental policies that have contributed to MSMEs’ growth and women’s participation are identified. The findings suggest ways to improve and support both the creation of more women-owned MSMEs in emerging countries, such as Ecuador, and the survival of existing male- and female-owned MSMEs.