bluuebunny/crossref_metadata_2025_split huggingface dataset:
An obesity model and corona multiomics analysis reveal high-density lipoprotein effects on lipid nanoparticle function
Authors: Liu Kai , Nilsson Ralf , Lázaro-Ibáñez Elisa , Miliotis Tasso , Lerche Michael , Ribeiro Rita Salgado , Strimfors Marie , Duàn Hanna , Ulander Johan , Lindén Daniel , Salvati Anna , Sabirsh Alan 
Published: September 2021

Abstract
Lipid nanoparticles (LNPs) are currently of great interest for therapeutic nucleic acid delivery. Lipid-based nanoparticles are, however, difficult to study analytically and so our understanding of the interaction between LNPs and biological systems remains obscure, particularly in terms of biomolecular corona formation and the impact this has on therapeutic efficacy and targeting. Ideally, we would like to engineer particles to acquire corona components that facilitate targeting, cargo delivery and improved safety. This requires studying the relationship between LNPs, biofluids and the resulting coronas. The particle-corona complexes are, however, fragile and biofluids also contain particles (such as lipoproteins) with sizes and biochemical characteristics similar to lipid nanoparticles, so contamination with biological components is a challenge. Here we describe a rapid, automated, and unbiased isolation method for LNP biomolecular coronas, coupled with proteomic and lipidomic analysis. Using these analytical methods, we systematically studied, in lean and obese rats, the variation in LNP-mediated mRNA delivery caused by individual physiology. A comprehensive multivariate model links LNP corona content to efficacy, identifying and validating high-density lipoproteins as a previously unidentified factor affecting particle efficacy.
Corrosion Inhibition Performance of Azelaic Acid Dihydrazide And Its Potential Predicted To Target The SARS-CoV-2 Spike Protein With Docking Study
Authors: Abdelmalek Matine , Barhoumi Ali , Bayadi Said , idrissi Mohammed El , Salah Mohammed , Tounsi Abdessamad , Ouardi El Mokhtar El , Abdallaoui Habib El Alaoui El , Zeroual Abdellah 
Published: September 2021

Abstract
The adsorption of azelaic acid dihydrazide as an environmentally friendly mild steel corrosion inhibitor on the iron surface was modelled in this study. We used density functional theory (DFT) calculations and Monte Carlo (MC) and Molecular dynamics (MD) simulations to illustrate the interactions engaged. The interaction of the azelaic acid derivatives with iron metal (Fe) was examined by DFT as a typical example of a corrosion prevention mechanism after the optimized molecular structures of these molecules were investigated. Structures, binding energies, Fikui's charge indicator, electron transfer, and chemical potential are all discussed, the presence of significant binding between the inhibitor and Fe metal is supported by analysis of the resultant complex. Then, in an acidic solution comprising 491,H2O, nine chlorine ions Cl-, and nine hydronium ions H3O+, molecular dynamic, Monte Carlo (MC) simulation were used to model the adsorption of azelaic acid dihydrazide on the iron Fe (110) surface. In addition, radial distribution function (RDF) and interaction energy (Ei) were evaluated in this work to further our understanding of interactions between azelaic acid dihydrazide and iron surfaces. Furthermore, we discovered that our inhibitors have an excellent ability to slow down the movement of corrosive particles in law temperature and thus to inhibit the metallic substrate against corrosive electrolyte, based on the temperature impact investigation. The result of density functional theory, Mont Carlo and molecular dynamic descriptors obtained were in good agreement with the experimental result.
Evaluation of Blood Glucose As A Biomarker For On Spot Detection of Bovine Trypanosomosis In Emergent Nations
Authors: Agrawal Vivek , Jaiswal Amit , Shukla Supriya , Mehta Hemant , Shakya Mukesh , Jatav Gaya Prasad , Jayraw Anant K. 
Published: September 2021

Abstract
Trypanosomosis or surra has been declared notifiable multispecies animal disease by World Organization for Animal Health. Diagnosis of trypanosomosis is a challenging task in field condition because none of reported signs is pathognomonic resulting in failure of treatment either inappropriate or delay in treatment. In spite of this, low hemoglobin level and hypoglycaemia are consistent hemato-biochemical changes in trypanosomosis. Therefore taking into consideration of both these two changes, tentative diagnosis of trypanosomosis could be done in low resource laboratory situated in rural area. With the development of eco and user friendly pen-side diagnostic, effective management of animal trypanosomosis could only be possible. Out of 123 buffaloes, 21 buffaloes found positive by PCR were showing average temperature 105.130F±0.983, blood glucose 32.319±9.760 and haemoglobin 8.60±1.485. Likewise Value of blood glucose (glucometer- 95% CI 68.488 to 77.141 and semiautomatic blood analyzer- CI 64.637 to 74.087) and haemoglobin (95% CI 95.743 to 97.314) were found statistically highly significant (P<0.001) with body temperature. Lower the blood glucose level in fevered animals may suspect the trypanosomosis. In these cases portable blood glucose meter may help the field veterinarian for on spot estimation of blood glucose level of fevered animals. An on spot estimation of lowered glucose level, anaemia and high temperature increases the probability of trypanosomosis. So that timely treatment of infected animal could be possible in rural area without wasting of time to get the report from full equipped laboratory. This might be the first time reported method for early diagnosis of trypanosomosis.
C/EBPβ Acetylation is Involved in Idiopathic Pulmonary Fibrosis
Authors: Zhou Jingzhu , Ji Xiuhai , fen yan , ding Hui 
Published: September 2021

Abstract
Background: IPF is a progressive lung disease, characterized by excessive deposition of ECM. C/EBPβ is involved in the development of pulmonary fibrosis. However, the regulation of C/EBPβ in the context of pulmonary fibrosis is not clear. The study is to identify the C/EBPβ acetylation in IPF．Methods: Lung from six IPF and six control samples were selected in this study. We investigated the expression of C/EBPβ in lungs with Immunochemistry. Moreover, the expression of C/EBPβ mRNA via Real Time-PCR and its protein expression via Western Blot were performed. Meanwhile, the levels of collagen-I and α-SMA as markers of pulmonary fibrosis were also determined by Western Blot. Furthermore, we confirmed the relationship between α-SMA and acetylated C/EBPβ by Co-Immunoprecipitation. Results: We found the elevated C/EBPβ mostly locating in fibroblast foci in lungs of IPF. And the expression of C/EBPβ RNA and protein were obviously increased in IPF (P <0.05), in which the proteins of α-SMA and collagen-I were enhanced (P <0.05). Furthermore, the stronger acetylation of C/EBPβ binging to the α-SMA gene was shown in lung fibrosis (P <0.05). Conclusions: The increased expression of C/EBPβ acetylation associated with α-SMA expression is involved in the development of pulmonary fibrosis.
AmphotericinB and Fluorocytosine Combined with Voriconazole for the Treatment of Non-HIV- and Non-Transplant-Associated Cryptococcal Meningitis: A Retrospective Study
Authors: Liu Junyu , Liu Jia , Su Xiaohong , Yang Lu , Wang Yijie , Wang Anni , Xu Xiaofeng , Li Min , Jiang Ying , Peng Fuhua 
Published: September 2021

Abstract
Background

This retrospective study aimed to explore the curative effect of amphotericin B (AMB) and fluorocytosine (5-FC) combined with voriconazole (VOR) in the induction period for the treatment of non-HIV- and non-transplant-associated cryptococcal meningitis (CM).
Methods

Clinical data from patients admitted to the Third Affiliated Hospital of Sun Yat Sen University from January 2011 to December 2020 were collected. The patients were stratified based on antifungal treatment methods in the induction period (group I with AMB + 5-FC + VOR, group II with AMB + 5-FC + FLU, group III with AMB + 5-FC).
Results

The length of hospital stay in group I was significantly shorter than those in group II (p = 0.000) and group III (p = 0.000). Among the patients who achieved CSF sterility within 10 weeks, the number of days to reach CSF sterility in group I was shorter than that in group II (p = 0.046). The incidence of hypokalemia in group I was significantly lower than those in group II (p = 0.003) and group III (p = 0.004). The incidence of gastrointestinal discomfort in group I was significantly lower than that in group II (p = 0.004).
Conclusion

AMB plus 5-FC combined with VOR for the induction treatment of non-HIV- and non-transplant-associated cryptococcal meningitis is a better choice than AMB plus 5-FC combined with FLU and AMB plus 5-FC. This new triple antifungal treatment method rapidly cleared the cryptococci from the CSF, substantially shortened the hospital stay, and decreased the incidences of hypokalemia and gastrointestinal discomfort.
Microfinance Institutions and Financial Sector Growth in Nigeria (1992-2018)
Authors: Ayodeji Emmanuel Abiodun , Ogundipe Adebayo Tunbosun 
Published: September 2021

Abstract
The extent to which microfinance bank institutions have contributed to the financial sector growth has not been well unraveled in the extant literature in Nigeria, hence, this study examined the effects of microfinance banks on financial sector growth in Nigeria. It further investigated the dynamic form of relationship between microfinance banks and financial sector growth in Nigeria covering a temporal scope 1992 to 2018. The model specification was formulated using financial sector GDP as the proxy for dependent variable, microfinance credit, deposits, assets and investment were used as proxies for microfinance banks institutions. Secondary data were sourced from CBN statistical Bulletin and analyzed using auto regressive distributed lag bound test and its corresponding short and long run coefficients. Finding revealed an inconclusive long run relationship between microfinance bank institutions and financial sector growth. Checking the individual variable coefficients in the short run, microfinance credit has significant positive effect while microfinance assets has insignificant effects on financial sector growth. In the long run, it was revealed that microfinance bank deposits and assets exert insignificant positive effects while microfinance credits have insignificant effect and investments have significant negative effects on financial sector growth. The study concluded that, in the long run, microfinance bank institutions exert positive and insignificant effects on financial sector growth in Nigeria. It was therefore recommended that, for microfinance bank institutions to impact significantly on financial sector growth in Nigeria, its credit should be increased and be more directed to the target individuals and the level of their investments should be geared up so as to engender growth of the financial sector in Nigeria. Furthermore, microfinance bank institutions should maintain its status quo on deposits and assets, however, improvement on them should be encouraged so as to enhance the growth of the financial sector in Nigeria
Vitamin D for Autism Spectrum Disorders:a Protocol for Systematic Review and Meta-analysis
Authors: wang Jing , Chu Xin , Hao Haoyu , Xuemei He , Chen Yu , Wang Min , Zou Zhuo , Yin Shuyue , Qin Dongdong , Liu Yun 
Published: September 2021

Abstract
IntroductionAutism spectrum disorder(ASD) is a neurodevelopmental disorder characterized by repetitive and stereotypic behavior, impaired social interactions and communications. An increasing number of reseachers suggested that vitamin D may be participated in the pathogenesis of ASD, and vitamin D deficiency may be one of the causes of ASD.Meanwhile, some studies have shown that vitamin D can improve the core symptoms in ASD children.On top of updating the latest systematic review on vitamin D supplementation and ASD, we aim to conduct the meta-analyses of trials on vitamin D3 supplementation and ASD. Besides, we will conduct for the first time subgroup analyses based on individual patient data collected from randomised controlled trials.Methods and analysisA systematic review and individual patient data meta-analysis on randomised placebo-controlled trials with a vitamin D3 intervention. All studies are searched from inception without time restriction. The addressed outcomes are 25-hydroxyvitamin D, Childhood Autism Rating Scaleas(CARS), Autism Behavior Checklist (ABC), Social Responsiveness Scale(SRS) and M-CHAT. Trial results will be reanalysed using Cox proportional hazard regression models and meta-analyses are performed. Cochran’s Q-Test and the I2 index will be used to statistically assess the level of heterogeneity, while sensitivity and subgroup analyses serve to identify potential causes of heterogeneity. Subgroup analyses will be conducted for age, sex, 25-hydroxyvitamin D level,vitamin D3 dosing, follow-up time,and the core symptoms of ASD. Publication bias will be assessed by funnel plots and Egger’s test. Ethics and disseminationEthical approval is not required since no human beings are involved in this systematic review. Results will be published in a peer-reviewed journal with open access. Strengths and limitations of this study1.Meta-analysis on vitamin D and ASD,we not only study the relationship between vitamin D deficiency and the occurrence of ASD, the preventive and therapeutic effects of vitamin D on ASD, but also the influence factors of vitamin D supplementation on the therapeutic effect of ASD. 2.Assess study quality, sources of heterogeneity and bias minimise the risk of bias and will gather reproducible results.3.Eligible data for meta-analysis may be limited.PROSPERO registration number:CRD42021264097
Non-Destructive Mercury Exposure Assessment in the Brandt's Hedgehog (Paraechinus Hypomelas): Spines As Indicators of Endogenous Concentrations
Authors: behrooz Reza Dahmardeh , Poma Giulia , Barghi Mandana 
Published: September 2021

Abstract
Due to its persistence, bioaccumulation characteristics and toxicity, environmental contamination with mercury (Hg) is of high concern for human health, living organisms and ecosystems, and its biological monitoring is highly relevant. In this study, the levels of total Hg were measured in organs, tissues and spines of 50 individuals of Brandt's hedgehog collected in Iran in 2019. The Hg median levels in kidneys, liver, muscle, and spines were 156, 47, 47 and 20 ng/g dry weight, respectively. The results showed a significant positive correlation between the levels of Hg in kidneys and liver (r = 0.519; p < 0.01) and in spines and muscle (r = 0.337, p < 0.01) and kidneys (r = 0.309, p < 0.05). Significant differences (p < 0.05) in Hg levels in organs and tissues were also observed depending on the sex, weight, length, and age of the individuals. In addition, the median levels of total Hg in kidneys of Brandt's hedgehogs from an agricultural ecotype were significantly higher (p < 0.05) than those collected from a forest ecotype, suggesting that the habitat could have a significant impact on animal contamination.
CXCL12/CXCR7/β-arrestin1 Biased Signal Promotes Epithelial-to-Mesenchymal Transition of Colorectal Cancer by Repressing Mirnas Through YAP1 Nuclear Translocation
Authors: Si Mahan , Song Yujia , Wang Xiaohui , Wang Dong , Liu Xiaohui , Qu Xianjun , Yu Xinfeng 
Published: September 2021

Abstract
Background: CXCR7 is an atypical chemokine receptor that transmits biased signal independent of G-protein activation. However, whether CXCL12/CXCR7 biased signal activation plays an essential role in colorectal cancer (CRC) progression and metastasis remains obscure. Methods: The functional role of CXCL12/CXCR7 biased signal in CRC was investigated by RNA-sequencing, Transwell assay and in vivo tumor xenografts. YAP1 nuclear translocation and molecular mechanisms were determined by cell transfection, luciferase activity assay, immunofluorescence, coimmunoprecipitation and immunohistochemistry and RT-qPCR analysis.Results: In this study, CXCR7 CXCL12/overexpression promotes Epithelial-to-mesenchymal transition (EMT) and upregulates the expression of stem marker doublecortin-like kinase 1 (DCLK1) in CRC cells with concurrent repression of miR-124-3p and miR-188-5p. Further luciferase assay prove that these miRNAs could regulate EMT by direct targeting vimentin and DCLK1. More importantly, CXCL12/CXCR7/β-arrestin1-mediated biased signal induces YAP1 nuclear translocation, which functions as a transcriptional repressor by interacting with Yin Yang 1 (YY1) and recruiting YY1 to the promoter of miR-124-3p and miR-188-5p. Pharmacological inhibitor of YAP1 recapitulates the anti-tumorigenesis and anti-metastasis effects of YAP1 depletion upon CXCR7 activation in tumor xenografts. Clinically, the expression of CXCR7 was positively correlated with nuclear YAP1 levels and EMT markers. Conclusions: Our findings revealed the novel role of YAP1 nuclear translocation in promoting EMT of CRC by repressing miR-124-3p and miR-188-5p through CXCL12/CXCR7/β-arrestin1 biased signal activation. These findings highlight the potential of targeting YAP1 nuclear translocation in hampering CXCL12/CXCR7 biased signal-induced metastasis of CRC.
Transcriptional Regulation of the Colorectal Cancer Stem Cell Markers, Nanog and Oct4, Induced by a Thermodynamic-Based Therapy Approach
Authors: Ghorbani Zakieh , Heidari Mansour , Jafarinia Mojtaba , Rohani Mahdi , Akbari Abolfazl 
Published: September 2021

Abstract
Background: Cancer stem cells (CSC) play a crucial role in tumorigenesis, recurrence, metastasis, and chemoresistanc. Some studies suggest that hyperthermia and photodynamic therapy (PDT) may be effective for cancer treatment, particularly when combined with other therapeutic approaches. However, the results are conflicting. Our aim was to evaluate the effect of hyperthermia combined with PDT on colorectal CSC viability and the gene expression of the CSC markers.Results: Cell viability decrased by PDT (P=0.015) and the combination therapy (P=0.006) but not hyperthermia alone (P=0.4) compared to control. Gene expression of CSC markers significantly decreased in all therapies. Conclusion: Hyperthermia combined with PDT was more efficient in eliminating tumors than hyperthermia or PDT alone.
Satisfaction in Population-Based Cancer Screening in a Chinese Rural High-Risk Population: The Yangzhong Early Diagnosis and Treatment of Upper Gastrointestinal Cancer
Authors: Feng Xiang , Zhu Jinhua , Hua Zhaolai , Zhou Qin , Shi Aiwu , Song Tongqiu , Yao Shenghua , Qian Dongfu , Chen Ru , Wang GuiQi , Wei Wenqiang , Zhou Jinyi 
Published: September 2021

Abstract
Background

Screening for upper gastrointestinal cancer (UGC) effectively reduces morbidity and mortality in gastric and oesophageal cancers. It is considered one of the effective measures for cancer control in China, but studies on its functional quality are lacking. Our study assessed the quality of screening service funded by Upper Gastrointestinal Cancer Early diagnosis and treatment (UGCEDAT) and its correlation in Yangzhong People’s hospital, China.
Methods

A cross-sectional study was conducted among 516 screening users at a screening centre in Yanghzong People’s hospital from April to July 2021. The service quality questionnaire (SERVQUAL) base on the service quality gap (SQG) model was adopted. We calculated the mean scores of perceptions and expectations and their gap. To determine the association between overall SQG and related features of participants, we used a multivariate logistic regression.
Results

The average scores of screening service users’ perceptions and expectations were 4.05 and 4.55, respectively. The SQG of five dimensions (tangibles, reliability, responsiveness, assurance and empathy) were negative, and the overall SQG was − 0.51. The responsiveness dimension had the largest gap, and tangibles had the smallest gap. Occupation status (AOR:0.575; CI:0.371–0.890), health self-assessment (AOR:4.965; CI:1.352–18.232), endoscopy experience (AOR:0.551; CI:0.377–0.806), distance from screening hospital (AOR:1.847; CI:1.249–2.733) and frequency of visit (AOR:1.646; CI:1.100-2.462) were associated with the overall SQG.
Conclusions

We observed a negative gap between perceptions and expectations of the function quality of screening service, implying a high dissatisfaction across different dimensions. Service providers should take adequate measures to bridge the dimension with the largest quality gap. Meanwhile, attention should be paid to identifying the influencing factors of the overall gap and the characteristics of dimensional expectations and perceived scores to improve the effectiveness of the intervention.
Healthcare Use in Patients With Cardiovascular Disease and Depressive Symptoms – The Impact of Internet-Delivered Cognitive Behavioural Therapy. A Secondary Analysis of a RCT
Authors: Mourad Ghassan , Lundgren Johan , Andersson Gerhard , Klompstra Mats Westas , Johansson Peter 
Published: September 2021

Abstract
Background: Depressive symptoms in patients with cardiovascular disease (CVD) can lead to increased healthcare use and cause an economical burden for society. Thus, interventions aiming to decrease depressive symptoms in these patients could reduce the use of healthcare services. In a previous study we reported that a 9-week internet-delivered cognitive behavioural therapy (iCBT) program (n=72) compared to an online discussion forum (ODF) (n=72) had moderate to large effect on depression in CVD outpatients. However, the associations between healthcare use and depressive symptoms were not reported in that study. The aim of this study was to describe and compare the effect of iCBT compared to ODF regarding healthcare use and to identify factors impacting healthcare use in these groups.Methods: Secondary analysis of a RCT. Data on healthcare use (including outpatient clinic/primary care contacts and hospital admissions) were retrieved from care data registries in five hospitals in Southeastern Sweden. Patients were predominantly males in both groups with a mean age of 61±13 and 64±12 years in iCBT and ODF respectively.Results: The year before the intervention, the iCBT group had a mean of 31 outpatient contacts per patient compared with 21 contacts the year after. The corresponding numbers for the ODF group were 37 and 25. The decrease was 32% in both groups and did not differ significantly between the groups.Regarding hospital admissions, the iCBT group had 0.8 admissions per patient the year before and 0.6 the year after the intervention, i.e. a decrease by 25%, whereas the ODF group had 1.1 and 0.6 admissions respectively, i.e. a decrease by 45%. The difference was not significant between the groups.Improvement in depressive symptoms post intervention was significantly associated with a decrease in number of outpatient contacts in the iCBT group. In the ODF group, better mental health-related quality of life post intervention was significantly associated with a decrease in number of hospital admissions.Conclusions: Reduced depressive symptom scores following intervention were associated with lower outpatient service use, but iCBT was not superior compared to ODF in decreasing healthcare use.Trial registration: The study was registered at ClinicalTrials.gov, NCT02778074.