bluuebunny/crossref_metadata_2025_split huggingface dataset:
Inorganic and organic phosphate solubilization potential of Stenotrophomonas maltophilia
Authors: Suliasih  , Widawati S 
Published: December 2021

Abstract
Most soils contain Phosphorus (P) in insoluble compounds as organic and inorganic forms which is unavailable to plants. Furthermore, phosphate solubilizing bacteria (PSB) play an important role in converting insoluble P to a bioavailable form through solubilizing and mineralizing inorganic or organic P. Therefore, this study aims to determine the ability of isolate a phosphate solubilizing bacterium (PSB), Stenotrophomonas maltophilia to solubilize inorganic and organic P. The bacterium was isolated from peat soil of West Kalimantan using Pikovskaya medium added with Ca3 (PO4)2 as the P source. The activity of organic P (acid phosphatase and phytase) was measured using p-nitrophenyl phosphate disodium (pNPP 0.115 M) and sodium phytate as the substrate of bacterial broth culture. Also, the optimization for inorganic and organic P solubilization by the bacterium was conducted. The maximum values of inorganic and organic phosphate solubilization was recorded to be 52.26 μg/mL and 44.51 U/mL (acid phosphatase), 0.13 U/mL (phytase) respectively. Optimum conditions found were temperature at 30 °C, pH 6.0 and in the presence of sucrose and beef extract, which serve as carbon and nitrogen sources, respectively. Therefore, it can probably be used as a biological fertilizer for plants.
Field evaluation of the combination of larvicide and rice stem immersion to improve lethal ovitrap effectiveness in dengue vector control
Authors: Pujiyanti A , Mujiyanto M , Setiyaningsih R , Kinansi R R , Susanti L , Mulyono A , Garjito T A , Trapsilowati W 
Published: December 2021

AbstractThe combination of temephos and attractants fromO.sativawas effective for lethal ovitrap in laboratory testing. However, the effectiveness of the field application needs further investigation. The study’s objective was to determine the influence of ovitrap installation sites, season variation, and attractant on lethal ovitrap efficacy. The study used a quasi-experimental design. Lethal ovitraps were installed indoors and outdoors in 102 houses and observed for 14 weeks. Statistical analysis was conducted with univariate analysis, independent t-test, and Mann-Whitney Test. The statistical analysis results showed no significant difference in positive ovitraps between LOA and LONA on indoor and outdoor applications. The number of positive ovitraps and trapped eggs was not affected by the attractant applied to the LO during indoor and outdoor observations. Lethal ovitrap can be used as a vector control instrument without the addition of attractants. The application of lethal ovitrap is more effective in the dry season when the mosquito population is lower than in the rainy season
Bioactivities of Lamiaceae, Myristicaceae, and Myrtaceae plant oils against Nilaparvata lugens Stâl. (Hemiptera: Delphacidae)
Authors: Mardiningsih T L , Rohimatun  
Published: December 2021

AbstractEssential oil (EO) plants have properties as botanical insecticides. Some of them come from the families of Lamiaceae, Myristicaceae, and Myrtaceae. A plant belonging to the Lamiaceae includes patchouli (Pogostemon cablin), while the Myristicaceae includes nutmeg(Myristica fragrans), and the Myrtaceae includes clove (Syzygium aromaticum) and cajuput (Melaleuca cajuputsubsp.cajuputi). This research aimed to evaluate toxicity, feeding inhibition, and oviposition deterrent from four EO plants from the Lamiaceae, Myristicaceae, and Myrtaceae to brown rice planthopperNilaparvata lugens(Hemiptera: Delphacidae), the main pest of rice. The results showed that cajuput and patchouli oil gave more potent toxicity toN. lugenswith LC50 and LC95 were 0.016% and 4.87% for cajuput oil and 0.019% and 5.25% for patchouli oil. Cajuput oil at 0.002% deterred oviposition 51.74% and inhibited feeding on 0.016% as many as 71.52% with strong enough criteria to the fourth instar nymphs ofN. lugens. Patchouli oil deterred oviposition from 0.002% as much as 61.15% and inhibited feeding on 0.019% as many as 68.73% with strong enough criteria. The highest oviposition deterrent was 83.2% on patchouli oil at the concentration of 0.188%, whereas the highest feeding inhibition was 82.78% on cajuput oil at the concentration of 0.169%.
Motivation, purpose, and purchasing frequency of honey consumption in West Java
Authors: Purnomo D , Bunyamin A , Gunawan W , Faizah N A , Danuwidjaja T G , Rohman L N , Annisa R 
Published: December 2021

Abstract
Indonesia is home to the greatest diversity of social bees in all over Asia, particularly species of the genus Apis. Thus, expanding the apiculture industry for commercial development is highly considerable. Although this industry has not become a special concern, the products of this industry are very popular among the Indonesian people, both for health, lifestyle, and other benefits. Research plays an essential role for good decision making, however, there is little research related to honey marketing in Indonesia. In this study, we observed the honey consumption of 246 respondents living in West Java by using online questionnaires and Decision Tree Classification to contribute to honey marketing research. This research shows that the motivation of the respondents in buying honey was merely for health reasons and the main purpose was for personal consumption. As for purchasing frequency, 86% of respondents purchased honey more than once a month. Then, a classification model of honey purchasing frequency based on respondents’ demographics which has an accuracy of 70.3% was built. The study results should be considered by the food industry and honey producers to emphasize consumer behaviour to formulate a better marketing strategy.
Genetic construction of HBsAg gene subgenotype B3 in Lactococcus lactis as hepatitis B vaccine candidate
Authors: Mustopa A Z , Putri H H , Kusdianawati  , Budiarto B R , Kusumawati A , Nurfatwa M , Ekawati N , Prastyowati A , Triratna L , Hertati A , Umami RN 
Published: December 2021

Abstract
Hepatitis B is an inflammatory liver disease caused by HBV (Hepatitis B Virus). Hepatitis B surface antigen (HBsAg) induces immune system forming antibodies. HBV subgenotype B3 is common in Asian Countries. Thus, the development of HBsAg subgenotype B3 vaccine was done because its prevalence is high in Indonesia (especially in Javanese) and other Asian countries. The research methods were preparation of the HBsAg gene subgenotype B3, cloning and transformation the HBsAg gene in Escherichia coli MC1061, and transformation in Lactococcus lactis (L. lactis). HBsAg gene subgenotype B3 was obtained from the pIDT-HBsAg subgenotype B3 plasmid. The HBsAg gene subgenotype B3 successfully cloned and transformed into E. coli MC1061 and L. lactis. The PCR results of the transformant E. coli MC1061 (pNZ8148-HBsAg subgenotype B3) colonies were found in colonies 8, 17, and 20 indicated by the presence of 1226 bps bands. 8 colonies were obtained from PCR results of L. lactis transformants (pNZ8148-HBsAg subgenotype B3). The construction of the HBsAg subgenotype B3 gene has 100% similarity compare to the hepatitis B virus isolated from Java on 1839. Therefore, the construction of pNZ8148-HBsAg subgenotype B3 using host cells L. lactis can be used as a vaccine candidate.
In vitro plant growth promoter assay of actinobacteria potential for tidal land farming
Authors: Alfiyah D , Ghulamahdi M , Lestari Y 
Published: December 2021

Abstract
The use of tidal land for agricultural still faces constraints, mainly due to high iron content. Actinobacteria produce bioactive compound with many functions. The aim of this work was to assess the growth of actinobacteria at various iron concentrations and its capability as plant growth promoter. Four actinobacteria isolates (Cal31t, Dbi28t, Crc32t and Cal24h) were grown at various iron concentrations. The isolates were examined for their capability to produce IAA and fix N2 under in vitro assay. The growth of actinobacteria under stress conditions was examined by cultivating them in ISP2 medium at pH 4, 3% NaCl, 750 mg.L−1 AlCl3 and 8 concentrations of FeCl3, i.e. 0, 500, 1000, 2000, 4000, 8000, 16000, 32000 mg.L−1. Actinobacteria isolates were able to grow under iron stress condition up to 32.000 mg.L−1. Both Cal3t and Dbi28t produced higher cell biomass compared with the other two tested isolates. All isolates produced IAA when grown under iron stress condition up to 4000 mg.L−1 of FeCl3, were able to grow under N-free medium and capable to produce ammonia at various concentrations. Crc32t produced the highest number of ammonia (0,354 mg.L−1). Cal31t and Crc32t isolates have the potency as plant growth promoter in tidal land farming.
Genetic diversity of multiple human immunodeficiency virus-1 (HIV-1) clades in West Papua
Authors: Widiyanti M , Adiningsih S , Kridaningsih T N 
Published: December 2021

Abstract
Human Immunodeficiency Virus (HIV) genetic variability is classified into types, groups, and subtypes. HIV is divided into 2 types, HIV-1 and HIV-2. HIV-1 was divided into groups M (Main), N (New), and O (Outlier). Group M was divided into 9 subtypes and 48 Circulating Recombinant Form (CRF). Understanding HIV-1 subtype distribution and epidemiology can assist preventive measures and clinical decisions. Sequence variation may affect drug resistance, disease progression, and transmission route. This study aimed to determine human immunodeficiency virus type-1 (HIV-1) subtypes which circulate in West Papua based on gene fragments encoding the glycoprotein-41 (gp41) of the HIV envelope. The descriptive-analytic method was attended at the VCT clinic in West Papua. Samples of blood plasma from patients with HIV were amplified using RT-PCR and nested PCR. Genetic variability was analyzed using DNA sequencing. The HIV-1 subtype was determined for 35 samples which 15 were from female patients and 20 were from male patients. 3 subtypes were detected: Circulating Recombinant Form AE (CRF01_AE, n=21), B (n=12), and 52_01B (n=2). Subtypes AE predominated in HIV-1 acquired heterosexually 100 % and clinical stage level III. Subtypes AE strains of group M viruses predominated in West Papua in HIV transmitted by heterosexual contact.
Nephroprotective and antioxidant effects of ethanol extract of Coprinus comatus mushroom fruit-bodies on streptozotocin-induced diabetic rat models
Authors: Ratnaningtyas N I , Hernayanti  , Ekowati N , Husen F 
Published: December 2021

AbstractDiabetes mellitus (DM) is a metabolic disease with an increasing prevalence. An increase in free radicals and AGEs can cause glomerular cell damage which can lead to inflammation and DM nephropathy. WHO has recommended DM treatment by using herbal medicines that have minimal side effects and have a lot of biological activity to prevent complications.Coprinus comatus(O.F. Mull.) is known as immunomodulatory, anti-inflammatory and antidiabetic agent. The research usedC. comatuscultivated in Cianjur. The basidiome of this mushroom has slightly oval and small with height of 8-12 cm and thickness of 2-3 cm. The research included six groups of male Wistar rats: Group 1 received no treatment, Groups 2–6 were administered 45 mg/kg BW streptozotocin once, Group 3 was administered 45 mg/kg BW metformin, Groups 4, 5, and 6 were administered 250, 500, and 750 mg/kg BW ofC. comatusfruit body ethanol extract respectively for 14 days. The superoxide dismutase (SOD), malondialdehyde (MDA), hepcidin and β2 microglobulin (B2M) levels were evaluated. Data were analyzed using analysis of variance and Duncan’s multiple-range tests. The results showed that dose of 500 mg was effective in increasing SOD and decreasing hepcidin, B2M and MDA levels.
Computational antigenic epitope prediction of clinical Indonesian Dengue virus NS1 protein
Authors: Pambudi S , Irawan D , Danny A , Widayanti T , Tarwadi  
Published: December 2021

AbstractThe identification of human Non-Structural-1 (NS1) protein epitopes will help us better understand Dengue virus (DENV) immunopathogenesis. In this study, several online and offline bioinformatic prediction tools were exploited to predict and analyze T-cell and B-cell epitopes of DENV NS1 consensus sequences originated from Indonesian clinical isolates. We identified a potential peptide at NS1155--163(VEDYGFGIF) which interact with MHC-I allele HLA-B*40:01 and showed high binding affinity (IC50) scores ranging between 63.8 nM to 183.9 nM for all Indonesian DENV serotypes. Furthermore, we have succeeded identified a region at the C-terminal of Indonesian DENV NS1 protein between 325--344 as part of discontinuous antigenic epitope which conserved for all serotypes. Our analyses showed this region could induce strong and persistent antibody against all DENV serotypes by interacting with MHC-I molecule and also recognized by B-cell receptor. The identification of DENV NS1 T-cell and B-cell epitopes may help in the development of a new vaccine, drug discovery, and diagnostic system to help eradicate dengue infection.
Expression comparison between two genes encoding CSF3 recombinant proteins having different codon composition at N-terminal in Escherichia coli
Authors: Dewi K S , Wahyuni F D , Salsabila S , Aminah  , Yanthi N D , Fuad A M 
Published: December 2021

Abstract
Colony-stimulating factor 3 (CSF3) is a glycoprotein with many therapeutic applications. In the Escherichia coli expression system, mRNA folding and stability near the translation initiation region (TIR) are known to influence protein expression significantly. We have successfully constructed the recombinant plasmid carrying genes encoding CSF3.1 and CSF3.2, which have different synonymous codon usage at N-terminal. In this study, we compared both expressions of CSF3.1 and CSF3.2 recombinant proteins in E. coli host. Recombinant plasmid pJ414-CSF3.1 and pJ414-CSF3.2 were transformed individually into E. coli NiCo21(DE3) competent cells by a heat-shock method, then spread on solid Lysogeny Broth (LB) medium containing ampicillin. Eight transformant colonies were selected and then expressed in 2xYT medium with the addition of IPTG inducer. Expression analysis was carried out using 15% SDS-PAGE gel. No significantly different band was observed in CSF3.1 protein expression compared to the negative control. In contrast, CSF3.2 protein can be expressed with a good amount at its expected size of 18 kDa. This result was strengthened by bioinformatics analysis which demonstrated the more open TIR of CSF3.2 than that of CSF3.1 Our study highlighted that AU-rich mRNA at the N-terminal is essential for efficient recognition of the ribosome binding site.
GO potato aeroponic seed production in Indonesia, producers’ perception toward the benefits and challenges
Authors: Sembiring A , Murtiningsih R , Sahat J P , Hartanto S 
Published: December 2021

Abstract
The high-quality potato seed availability in Indonesia always to be attempted continuously by Indonesian governments to encourage national potato production optimization. One of the efforts is through aeroponic potato seed production. The G0 (zero generation seed) aeroponic potato produces a better yield and healthier tuber compare to G0 conventional potato production. IVegRI has undertaken studies related to G0 potato aeroponic. The results have been disseminated and some of them have been adopted by the potato producers in various regions in Indonesia. The study aimed to investigate the potato aeroponic seed production in Indonesia based on the producers’ perception of the benefits and challenges. The study was conducted from April to December 2017, in 8 districts from 6 provinces, engaging 18 G0 aeroponic potato producers. Data were analyzed descriptively. The resulting study showed that G0 aeroponic producers perceived the production of G0 aeroponic potato as more profitable compared to G0 conventional. The advantages covered: the production was higher, the yield tuber was healthier and sterile, and also it could be produced anytime. Meanwhile, challenges faced were: initially investment cost was quite expensive, electricity installation frequently was unstable and, the challenge concocted a suitable potato aeroponic nutrition.
Nitrogen (N2) fixation activity under different oxygen concentration of methanotrophic bacteria isolated from rice fields and their molecular identification
Authors: Rusmana I , Akhdiya A , Sagala B T 
Published: December 2021

Abstract
Rice fields are a significantly sources of atmospheric methane. Methanotrophic bacteria are unique in their ability to utilize methane as a sole carbon source and their ability to fix N2. This research successfully characterized N2 fixation activity under different oxygen concentrations of methanotrophic bacteria isolated from rice fields. From 19 tested isolates, four isolates performed activity to fix N2. They could fix N2 on different concentration of air saturation (10 % up to 100%). The growth of methanotrophs is not directly corelated with the N2 fixation activity, and their N2 fixation activities are affected by O2 concentrations. The BGM 3 and BGM 9 isolates had very good N2 fixation activity. Their activities were increased by increasing air saturation up to 50% (approximately 10% O2), but then decrease by increasing air saturation from 50% (approximately 10% O2) to 100% (approximately 20% O2). However, the highest N2 fixation activity was performed by the BGM 9 isolate at 30% air saturation (approximately 6% O2), and the isolate was identified as Methylococcus capsulatus. This information can support application of the isolates to achieve sustainable and environmentally friendly agricultural system.
EUROPEAN ITS PLATFORM: RETHINKING ITS
Authors: Giamarelos Orestis , Reiff Tobias 
Published: December 2021

Abstract
The expectations placed on the existing European transport infrastructure are increasing due to the growing mobility needs of the population on the one hand and the rising volume of freight traffic on the other. The aim of the European ITS Platform is to increase the efficiency of the TEN-T core network through a better use of the existing infrastructure by implementing Intelligent Transport Systems (ITS). It fosters interoperability and the development of uniform technical standards by monitoring and disseminating the results delivered by the five CEF ITS Road Corridor Projects.
One major outcome is the drafting of the “Reference Handbook for harmonized ITS Core Service Deployment in Europe”. This digital freely available handbook comprises a series of guidance and advice for use by road authorities and operators to support them in the development of their strategic approach, design development, deployment, installation and operation of ITS and remain compliant with European legislation. Its key features include: Deployment Guidance for 14 different ITS Services, including DATEX reference profiles, Guidance on the provision and use of traffic information provided under the European Directive (2010/40/EU), C-ITS developments, Knowledge gained from the collection of 100 Best Practices etc.
Construction Of Express Roads In Latvian State Main Road Network
Authors: Bērziņa Dace 
Published: December 2021

Abstract
Latvian State Road Development Strategy for 2020 – 2040 was approved in the year 2020. It is a vision that includes long-term perspectives, strategic goals, tasks and priorities for road network development, and it is created for more efficient planning of state road network development, as well as, attracting additional funding for state owned roads.
The aim of the Strategy 2040 is to create an efficient road network ensuring that the bypass of the Riga City is accessible within two hours from every national and regional development centre in Latvia. Development centres would be accessible within 45 minutes from every Latvian urban settlement along state regional and local roads.
The strategic task is to create sections of express roads in the total length of 1000 kilometres thus improving traffic safety and reducing the emissions of greenhouse gases.
When creating the Strategy 2040 both the changes in traffic and road use and the changes in the location of population were studied. The Strategy foresees that high-speed express road sections would connect the Riga City with the biggest cities. The proposed plan of road network development is created with the aim to cover as large a population as possible.
Special attention is paid to the Riga City, as it serves as the central hub for Latvian and Baltic transportation. After the implementation of the Strategy 2040, the Riga City would be reached within 30 minutes from the nearest development centres.
Advantages Of Filler And Surfactant Additive To Improve Moisture Sensitivity Of Hot Mix Asphalt Mixtures.
Authors: Alamrew Asres Simeneh , Mollenhauer Konrad 
Published: December 2021

Abstract
This research investigated the effect of mineral composition of aggregate on moisture sensitivity of bituminous mixtures and explored the benefits of hydrated lime filler and Wetfix BE surfactant additive to improve the resistance of the mix against moisture sensitivity. Basalt, quartzite, and limestone aggregates were selected based on their different mineralogy and 70 -100 penetration graded bitumen binders used during the study. Four laboratory tests the rolling bottle, shaking abrasion, pull-off tensile strength and indirect tensile strength tests were applied to study the effects of aggregate minerals and benefits of hydrated lime and Wetfix BE. Statistical analysis using Two-way ANOVA test conducted for each test to check the outcome significance. Results from each test revealed that mineral composition of aggregate have significant effects on the moisture resistance performance of bituminous mixtures and hydrated lime filler and Wetfix BE surfactant additives have advantages to improve the performance of bituminous mixture against moisture sensitivity and improves the long-term performance of asphalt mix.
Research Of Asphalt Concrete Frost Resistance According To Fatigue Resistance And Stiffness Criteria
Authors: Paulovskis Nauris 
Published: December 2021

Abstract
The formation of snow cover has been unsustainable in recent decades, which means that freezing and thawing are becoming more frequent in Latvia and that is one of the reasons why asphalt concrete frost resistance matters. Asphalt concrete beams were exposed to multiple freeze-thaw cycles and afterwards tested on a 4-point bending test for stiffness and fatigue resistance. Frost resistance testing for aggregates and concrete is a popular and widely described and used test method worldwide, but there is no such testing standard for asphalt concrete. This study is aimed to develop a methodology for determining the frost resistance of asphalt concrete by adapting the requirements of cement concrete standart AASHTO C666, as well as to experimentally evaluate the effect of frost resistance on the fatigue strenght and stiffness of asphalt concrete. The results show significant correlation between asphalt concrete void content and fatigue life after multiple freeze-thaw cycles and correlation between the amount of freeze-thaw cycles and asphalt concrete fatigue life.
Viaduct Over The Lorupe Ravine
Authors: Dziedātāja Indra 
Published: December 2021

Abstract
On the 20th July 2018, it was the 50th anniversary of the day when the Lorupe viaduct was opened. Its idea, design and construction were a significant turning point in bridge construction in the whole Soviet Union. In the comparatively small Republic of Latvia it was a unique project that required a lot of boldness and skills of road workers. Therefore, construction of this bridge is memorable, especially since several bridges were built using this technology later in the former Soviet Union. Latvian road workers with their courage, creative and meticulous work implemented a European scale project in difficult, constrained circumstances.