bluuebunny/crossref_metadata_2025_split huggingface dataset:
Hepatoblastoma: glutamine depletion hinders cell viability in the embryonal subtype but high GLUL expression is associated with better overall survival
Authors: Schmidt Andreas , Armento Angela , Bussolati Ovidio , Chiu Martina , Ellerkamp Verena , Scharpf Marcus O. , Sander Philip , Schmid Evi , Warmann Steven W. , Fuchs Jörg 
Published: July 2021

Abstract
Purpose
Glutamine plays an important role in cell viability and growth of various tumors. For the fetal subtype of hepatoblastoma, growth inhibition through glutamine depletion was shown. We studied glutamine depletion in embryonal cell lines of hepatoblastoma carrying different mutations. Since asparagine synthetase was identified as a prognostic factor and potential therapeutic target in adult hepatocellular carcinoma, we investigated the expression of its gene ASNS and of the gene GLUL, encoding for glutamine synthetase, in hepatoblastoma specimens and cell lines and investigated the correlation with overall survival.

Methods
We correlated GLUL and ASNS expression with overall survival using publicly available microarray and clinical data. We examined GLUL and ASNS expression by RT-qPCR and by Western blot analysis in the embryonal cell lines Huh-6 and HepT1, and in five hepatoblastoma specimens. In the same cell lines, we investigated the effects of glutamine depletion. Hepatoblastoma biopsies were examined for histology and CTNNB1 mutations.

Results
High GLUL expression was associated with a higher median survival time. Independent of mutations and histology, hepatoblastoma samples showed strong GLUL expression and glutamine synthesis. Glutamine depletion resulted in the inhibition of proliferation and of cell viability in both embryonal hepatoblastoma cell lines. ASNS expression did not correlate with overall survival.

Conclusion
Growth inhibition resulting from glutamine depletion, as described for the hepatoblastoma fetal subtype, is also detected in established embryonal hepatoblastoma cell lines carrying different mutations. At variance with adult hepatocellular carcinoma, in hepatoblastoma asparagine synthetase has no prognostic significance.
Invasive lizard has fewer parasites than native congener
Authors: Tomé Beatriz , Harris D. James , Perera Ana , Damas-Moreira Isabel 
Published: July 2021

AbstractInvasive species can carry parasites to introduced locations, which may be key to understand the success or failure of species establishment and the invasive potential of introduced species. We compared the prevalence and infection levels of haemogregarine blood parasites between two sympatric congeneric species in Lisbon, Portugal: the invasive Italian wall lizard (Podarcis siculus) and the native green Iberian wall lizard (Podarcis virescens). The two species had significant differences in their infection levels: while P. virescens had high prevalence of infection (69.0%), only one individual of P. siculus was infected (3.7%), and while P. virescens exhibited an average intensity of 1.36%, the infected P. siculus individual had an infection rate of only 0.04%. Genetic analyses of 18S rRNA identified two different haemogregarine haplotypes in P. virescens. Due to the low levels of infection, we were not able to amplify parasite DNA from the infected P. siculus individual, although it was morphologically similar to those found in P. virescens. Since other studies also reported low levels of parasites in P. siculus, we hypothesize that this general lack of parasites could be one of the factors contributing to its competitive advantage over native lizard species and introduction success.
High temperatures during microsporogenesis fatally shorten pollen lifespan
Authors: Iovane Maurizio , Aronne Giovanna 
Published: July 2021

AbstractMany crop species are cultivated to produce seeds and/or fruits and therefore need reproductive success to occur. Previous studies proved that high temperature on mature pollen at anther dehiscence reduce viability and germinability therefore decreasing crop productivity. We hypothesized that high temperature might affect pollen functionality even if the heat treatment is exerted only during the microsporogenesis. Experimental data on Solanum lycopersicum ‘Micro-Tom’ confirmed our hypothesis. Microsporogenesis successfully occurred at both high (30 °C) and optimal (22 °C) temperature. After the anthesis, viability and germinability of the pollen developed at optimal temperature gradually decreased and the reduction was slightly higher when pollen was incubated at 30 °C. Conversely, temperature effect was eagerly enhanced in pollen developed at high temperature. In this case, a drastic reduction of viability and a drop-off to zero of germinability occurred not only when pollen was incubated at 30 °C but also at 22 °C. Further ontogenetic analyses disclosed that high temperature significantly speeded-up the microsporogenesis and the early microgametogenesis (from vacuolated stage to bi-cellular pollen); therefore, gametophytes result already senescent at flower anthesis. Our work contributes to unravel the effects of heat stress on pollen revealing that high temperature conditions during microsporogenesis prime a fatal shortening of the male gametophyte lifespan.
Reduced dose folinic acid rescue after rapid high-dose methotrexate clearance is not associated with increased toxicity in a pediatric cohort
Authors: Niinimäki Riitta , Aarnivala Henri , Banerjee Joanna , Pokka Tytti , Vepsäläinen Kaisa , Harila-Saari Arja 
Published: July 2021

Abstract
Purpose
Low doses of folinic acid (FA) rescue after high-dose methotrexate (HD-MTX) have been associated with increased toxicity, whereas high doses may be related to a decreased antileukemic effect. The optimal dosage and duration of FA rescue remain controversial. This study was designed to investigate, whether a shorter duration of FA rescue in the setting of rapid HD-MTX clearance is associated with increased toxicity.

Methods
We reviewed the files of 44 children receiving a total of 350 HD-MTX courses during treatment for acute lymphoblastic leukemia according to the NOPHO ALL-2000 protocol. Following a 5 g/m2 HD-MTX infusion, pharmacokinetically guided FA rescue commenced at hour 42. As per local guidelines, the patients received only one or two 15 mg/m2 doses of FA in the case of rapid MTX clearance (serum MTX ≤ 0.2 μmol/L at hour 42 or hour 48, respectively). Data on MTX clearance, FA dosing, inpatient time, and toxicities were collected.

Results
Rapid MTX clearance was observed in 181 courses (51.7%). There was no difference in the steady-state MTX concentration, nephrotoxicity, hepatotoxicity, neutropenic fever, or neurotoxicity between courses followed by rapid MTX clearance and those without. One or two doses of FA after rapid MTX clearance resulted in a 7.8-h shorter inpatient time than if a minimum of three doses of FA would have been given.

Conclusion
A pharmacokinetically guided FA rescue of one or two 15 mg/m2 doses of FA following HD-MTX courses with rapid MTX clearance results in a shorter hospitalization without an increase in toxic effects.
COVID-19 and the Demand for Online Grocery Shopping: Empirical Evidence from the Netherlands
Authors: Baarsma Barbara , Groenewegen Jesse 
Published: July 2021

AbstractThere has been a pronounced increase in online shopping since the start of the COVID-19 pandemic. We study the effect of the pandemic on demand for online grocery shopping specifically, using municipality-level data from a Dutch online supermarket. We find that an additional hospital admission increased app traffic by 7.3 percent and sales per order by 0.31 percent. Local hospital admissions do not correlate with the variety of groceries ordered, but online search behavior does, suggesting that hoarding behavior is driven by the general perception and impact of the virus rather than local conditions. Local COVID-19 conditions also have different effects in urban versus non-urban municipalities, with local hospital admissions increasing app traffic in urban areas but lowering sales per order as compared to non-urban areas. It remains to be seen whether the demand for online grocery shopping will permanently increase as a result of the COVID-19 pandemic.
Comparative analysis of the morphological and biomechanical properties of normal cornea and keratoconus at different stages
Authors: Wu Ying , Guo Li-Li , Tian Lei , Xu Ze-Quan , Li Qian , Hu Jian , Huang Yi-Fei , Wang Li-Qiang 
Published: July 2021

Abstract
Purpose
To compare the morphological and biomechanical properties of normal cornea and keratoconus at different stages.

Methods
A total of 408 patients (517 eyes) with keratoconus were included in this study. According to the Topographic Keratoconus (TKC) grading method, keratoconus was divided into stage I (TKC = 1, 130 eyes), stage II (TKC = 1–2, 2, 164 eyes), stage III (TKC = 2–3, 3, 125 eyes) and stage IV (TKC = 3–4, 4, 98 eyes). A total of 158 normal subjects (158 eyes) were recruited as the normal group. The corneal morphological parameters and biomechanical parameters were obtained with Scheimpflug tomography (Pentacam) and corneal visualization Scheimpflug technology (Corvis ST), and the receiver operating characteristic (ROC) curves were drawn.

Results
Each corneal morphological and most biomechanical parameters of the keratoconic eyes were significantly different from those of the normal eyes in this study (p < 0.001). ROC curve demonstrated that most parameters in this study showed high efficiency in diagnosing keratoconus (the area under the ROC (AUC) was > 0.9), with the Belin-Ambrósio deviation (BAD-D) and Tomographic and Biomechanical Index (TBI) showing higher efficiency. The efficiency of BAD-D and TBI was high in differentiating keratoconus at different stages (AUC > 0.963). The comparison of ROC curves of keratoconus at different stages did not reveal statistically significant differences for TBI.

Conclusion
BAD-D and TBI can effectively diagnose stage I keratoconus. Moreover, the efficiency of TBI is the same in diagnosing keratoconus at all stages, while the diagnostic efficiency of other parameters increases with the increase in keratoconus stages.
Differences in Adolescents’ Alcohol Use and Smoking Behavior between Educational Tracks: Do Popularity Norms Matter?
Authors: Peeters M. , Laninga-Wijnen L. , Veenstra R. 
Published: July 2021

AbstractExplanations about differences in drinking and smoking rates between educational tracks have so far mainly focused on factors outside the classroom. The extent to which these behaviors are rewarded with popularity within a classroom—so called popularity norms—and their interaction with individual characteristics could explain the observed differences in risk behavior. 1860 adolescents (Mage = 13.04; 50% girls) from 81 different classrooms reported three times during one academic year about their own and their classmates behavior. Overall, in vocational tracks popularity norms for alcohol and smoking were more positive and predicted classroom differences in alcohol and smoking. Knowledge about classroom processes can advance the field in unraveling the functional aspects of risk behavior in adolescence. Preregistration: The hypotheses and the analytical plan of this study were preregistered under number #39136 (https://aspredicted.org/blind.php?x=gx77p6).
Air Learning: a deep reinforcement learning gym for autonomous aerial robot visual navigation
Authors: Krishnan Srivatsan , Boroujerdian Behzad , Fu William , Faust Aleksandra , Reddi Vijay Janapa 
Published: July 2021

AbstractWe introduce Air Learning, an open-source simulator, and a gym environment for deep reinforcement learning research on resource-constrained aerial robots. Equipped with domain randomization, Air Learning exposes a UAV agent to a diverse set of challenging scenarios. We seed the toolset with point-to-point obstacle avoidance tasks in three different environments and Deep Q Networks (DQN) and Proximal Policy Optimization (PPO) trainers. Air Learning assesses the policies’ performance under various quality-of-flight (QoF) metrics, such as the energy consumed, endurance, and the average trajectory length, on resource-constrained embedded platforms like a Raspberry Pi. We find that the trajectories on an embedded Ras-Pi are vastly different from those predicted on a high-end desktop system, resulting in up to$$40\%$$40%longer trajectories in one of the environments. To understand the source of such discrepancies, we use Air Learning to artificially degrade high-end desktop performance to mimic what happens on a low-end embedded system. We then propose a mitigation technique that uses the hardware-in-the-loop to determine the latency distribution of running the policy on the target platform (onboard compute on aerial robot). A randomly sampled latency from the latency distribution is then added as an artificial delay within the training loop. Training the policy with artificial delays allows us to minimize the hardware gap (discrepancy in the flight time metric reduced from 37.73% to 0.5%). Thus, Air Learning with hardware-in-the-loop characterizes those differences and exposes how the onboard compute’s choice affects the aerial robot’s performance. We also conduct reliability studies to assess the effect of sensor failures on the learned policies. All put together, Air Learning enables a broad class of deep RL research on UAVs. The source code is available at: https://github.com/harvard-edge/AirLearning.
An infinite family of higher-order difference operators that commute with Ruijsenaars operators of type A
Authors: Noumi Masatoshi , Sano Ayako 
Published: July 2021

AbstractWe introduce a new infinite family of higher-order difference operators that commute with the elliptic Ruijsenaars difference operators of typeA. These operators are related to Ruijsenaars’ operators through a formula of Wronski type.
Rectangular surface code under biased noise
Authors: Lee Jonghyun , Park Jooyoun , Heo Jun 
Published: July 2021

AbstractTo date, the surface code has become a promising candidate for quantum error correcting codes because it achieves a high threshold and is composed of only the nearest gate operations and low-weight stabilizers. Here, we have exhibited that the logical failure rate can be enhanced by manipulating the lattice size of surface codes that they can show an enormous improvement in the number of physical qubits for a noise model where dephasing errors dominate over relaxation errors. We estimated the logical error rate in terms of the lattice size and physical error rate. When the physical error rate was high, the parameter estimation method was applied, and when it was low, the most frequently occurring logical error cases were considered. By using the minimum weight perfect matching decoding algorithm, we obtained the optimal lattice size by minimizing the number of qubits to achieve the required failure rates when physical error rates and bias are provided .
Comparison of LPWAN Technologies: Cost Structure and Scalability
Authors: Hossain Mohammad Istiak , Markendahl Jan I. 
Published: July 2021

AbstractSmall-scale commercial rollouts of Cellular-IoT (C-IoT) networks have started globally since last year. However, among the plethora of low power wide area network (LPWAN) technologies, the cost-effectiveness of C-IoT is not certain for IoT service providers, small and greenfield operators. Today, there is no known public framework for the feasibility analysis of IoT communication technologies. Hence, this paper first presents a generic framework to assess the cost structure of cellular and non-cellular LPWAN technologies. Then, we applied the framework in eight deployment scenarios to analyze the prospect of LPWAN technologies like Sigfox, LoRaWAN, NB-IoT, LTE-M, and EC-GSM. We consider the inter-technology interference impact on LoRaWAN and Sigfox scalability. Our results validate that a large rollout with a single technology is not cost-efficient. Also, our analysis suggests the rollout possibility of an IoT communication Technology may not be linear to cost-efficiency.
SARS-CoV-2 anti-nucleocapsid assay performance in healthcare workers at baseline and 6 months
Authors: Kerr Colm , Allen Niamh , Hughes Gerry , Kelly Martina , O’Rourke Fiona , Lynagh Yvonne , Dunne Jean , Crowley Brendan , Conlon Niall , Bergin Colm 
Published: July 2021

Abstract
Introduction
Serological SARS-CoV-2 assays have an important role in guiding the pandemic response. This research aimed to compare the performance of 2 antinucleocapsid assays.

Methods
Serum from 49 HCWs was analysed at baseline and 6 months using the Abbott diagnostics SARS-CoV-2 IgG assay and the Roche Diagnostics Elecsys Anti-SARS-CoV-2 total antibody assay.

Results
At baseline, 14/49 participants (29%) demonstrated antibody reactivity using the Abbott assay. At 6 months, 4/14 participants (29%) continued to demonstrate reactivity. A total of 14/49 (29%) participants had detectable antibodies at baseline using the Roche assay. In total, 13/14 (93%) of participants demonstrated antibody reactivity at 6 months. The Abbott assay showed a statistically significant difference in the signal-to-threshold values of baseline reactive samples when repeated at 6 months (p = 0.001). This was not seen with the Roche assay (p = 0.51).

Conclusion
In this small study, the Roche Diagnostics Elecsys Anti-SARS-CoV-2 total antibody assay appears superior in performance to the Abbott diagnostics SARS-CoV-2 IgG assay in accurately detecting participants with a history of confirmed COVID-19 disease at 6 months follow-up. This finding should be born in mind in the planning of future seroprevalence studies, especially when considering the use of anti-nucleocapsid assays.
Morphometric characteristics of the sphenoid sinus and potential influencing factors: a retrospective assessment using cone beam computed tomography (CBCT)
Authors: Singh Pradeep , Hung Kuofeng , Ajmera Deepal Haresh , Yeung Andy Wai Kan , von Arx Thomas , Bornstein Michael M. 
Published: July 2021

AbstractThe present study aimed to evaluate the morphological characteristics of the sphenoid sinus (SS), and the impact of potential influencing factors on the morphometric features using CBCT imaging. CBCT scans of 148 patients, aged between 15 and 85 (32.88 ± 15.33) years were retrospectively evaluated. DICOM files from the CBCT scans were imported into semi-automatic software and the SS of each patient was assessed for the morphological characteristics including configuration, symmetry, extension, shape, septation, volume, and maximum diameter. Furthermore, potential influencing factors such as age, gender, side, and sinus condition were analysed. A significant association was observed between sinus extension and age. Septation was also found to be significantly associated with age, gender and sinus condition. Besides, sinus volume was significantly associated with gender and sinus condition. No significant influence of shape and side on the morphometric features was noticed. The average volume and diameter of the SS were 6576.92 ± 3748.12 mm3 and 30.48 ± 9.28 mm, respectively. In conclusion, the present findings indicate that age, gender and sinus condition have a significant impact on the morphometric characteristics of the SS. Mature sinuses exhibit a post-sellar extension pattern until middle age. In addition, males, and sinuses with healthy sinus condition have larger volumes compared to females and pathological sinuses.
Multi-Objective Optimization of WEDM of Aluminum Hybrid Composites Using AHP and Genetic Algorithm
Authors: Kumar Amresh , Grover Neelkanth , Manna Alakesh , Kumar Raman , Chohan Jasgurpreet Singh , Singh Sandeep , Singh Sunpreet , Pruncu Catalin Iulian 
Published: July 2021

AbstractAluminum hybrid composites have the potential to satisfy emerging demands of lightweight materials with enhanced mechanical properties and lower manufacturing costs. There is an inclusion of reinforcing materials with variable concentrations for the preparation of hybrid metal matrix composites to attain customized properties. Hence, it is obligatory to investigate the impact of different machining conditions for the selection of optimum parameter settings for aluminum-based hybrid metal matrix composite material. The present study aims to identify the optimum machining parameters during wire electrical discharge machining of samples prepared with graphite, ferrous oxide, and silicon carbide. In the present research work, five different process parameters and three response parameters such as material removal rate, surface roughness, and spark Gap are considered for process optimization. Energy-dispersive spectroscopy and scanning electron microscopy analysis reported the manifestation of the recast layer. Analytical hierarchy process and genetic algorithm have been successfully implemented to identify the best machining conditions for hybrid composites.
Coronavirus-related health literacy and perceived restrictiveness of protective measures among community-dwelling older persons in Finland
Authors: Eronen Johanna , Paakkari Leena , Portegijs Erja , Rantanen Taina 
Published: July 2021

Abstract
Background
Older people with limited health literacy may encounter difficulties in finding relevant information on COVID-19, understanding its relevance, and complying with recommended protective measures. Complying with such recommendations has required older as well as younger persons to change their daily lives in ways that have reduced their opportunities for engaging in many activities meaningful to them.

Aims
To find out from what sources older people have obtained information on protective measures, the level of their coronavirus-related health literacy (CHL), and whether CHL is associated with their perceptions of the restrictiveness of coronavirus-related protective measures.

Methods
696 Finnish men and women aged over 77 answered a mailed questionnaire on their CHL, sources of information and perceptions of the restrictiveness of the recommended protective measures. The association of CHL with perceived restrictiveness was studied using multinomial logistic regression analysis.

Results
Television and newspapers were the most frequently reported sources of information. Reporting high confidence in the ability to assess how one’s behavior influences coronavirus infection risk was associated with higher odds of perceiving the protective measures to be highly restrictive, when controlling for age, gender, and difficulty in using digital devices (OR 3.21, 95% CI 1.09, 9.46).

Discussion
Participants who reported being highly confident about their ability to appraise the influence of their behavior on their susceptibility to coronavirus infection were more likely to perceive that the recommended protective measures had restricted their daily lives.

Conclusions
Health literacy plays a role in complying with recommended restrictions.
The Main Progress of Perovskite Solar Cells in 2020–2021
Authors: Wu Tianhao , Qin Zhenzhen , Wang Yanbo , Wu Yongzhen , Chen Wei , Zhang Shufang , Cai Molang , Dai Songyuan , Zhang Jing , Liu Jian , Zhou Zhongmin , Liu Xiao , Segawa Hiroshi , Tan Hairen , Tang Qunwei , Fang Junfeng , Li Yaowen , Ding Liming , Ning Zhijun , Qi Yabing , Zhang Yiqiang , Han Liyuan 
Published: July 2021

AbstractPerovskite solar cells (PSCs) emerging as a promising photovoltaic technology with high efficiency and low manufacturing cost have attracted the attention from all over the world. Both the efficiency and stability of PSCs have increased steadily in recent years, and the research on reducing lead leakage and developing eco-friendly lead-free perovskites pushes forward the commercialization of PSCs step by step. This review summarizes the main progress of PSCs in 2020 and 2021 from the aspects of efficiency, stability, perovskite-based tandem devices, and lead-free PSCs. Moreover, a brief discussion on the development of PSC modules and its challenges toward practical application is provided.