bluuebunny/crossref_metadata_2025_split huggingface dataset:
Activation of the JAK1 / STAT1 Signaling Pathway is Associated with Prdx6 Expression Levels in Human Epididymis Epithelial Cells
Authors: Li Jianyuan , Shi Hui , Liu Xiaoyu , Wang Yanwei , Wang Haiyan , Pan Bochen 
Published: August 2021

Abstract
I. Background: Peroxiredoxin 6 (Prdx6) is widely expressed in mammalian tissues. Our previous study demonstrated that Prdx6 was expressed in human epididymis and spermatozoa, and the protective role of Prdx6 in human spermatozoa was also reported. In this study, we demonstrate the potential role and mechanism of Prdx6 in human epididymis epithelial cells (HEECs).II. Methods and Results: Western blotting was used to measure expression levels of key proteins in the JAK / STAT signaling pathway. Digital gene expression analysis (DGE) was used to identify gene expression patterns in control HECs and in HECs after Prdx6-RNA interference (P6-RNAi). The DGE analysis identified 589 up-regulated and 314 down-regulated genes (including Prdx6) in Prdx6-RNAi (P6-RNAi) HEECs. Thirteen significantly different pathways were identified between the two groups, with the majority different expressed genes belonging to the CCL, CXCL, IL, and IFIT families. In particular, the expression levels of IL6, IL6ST, and eighteen IFN related genes were significantly increased in the condition of the down-regulated expression of Prdx6. Compared to control HEECs, the expression levels of JAK1, STAT1, phosphorylated JAK1 and STAT1 were significantly increased, while the expression levels of SOCS3 was significantly decreased in P6-RNAi HEECs. The Malondialdehyde (MDA) level and total antioxidant capacity in P6-RNAi HEECs were significantly increased and decreased compared to that of control, respectively. III. Conclusions: We speculated that knockdown of Prdx6 resulted in higher levels of ROS in HEECs, which in turn, activated the JAK1 / STAT1 signaling pathway induced by IL-6 receptor and IFN.
ANLN and Lung Adenocarcinoma Prognosis and Immune Infiltration Research
Authors: Ru Meihua , Liu Ying-Bo , Tian Zhi-Hui , GODJE IVAN STEVE GODJE , Li Jian-Qiang 
Published: August 2021

Abstract
Objective: This study aims to explore the role of aniline-actin binding protein (ANLN) in the prognosis of lung adenocarcinoma (LUAD) and its role in immune infiltration, and to provide new ideas for clinical diagnosis and treatment.Methods: In this study, we applied bioinformatics methods to analyze the expression pattern and prognostic value of ANLN in LUAD ,and confirmed its independent prognostic value through Cox analysis. TIMER was used to evaluate the correlation between ANLN and tumor infiltrating immunity. LinkedOmics was used to study the co-expressed genes and functional networks related to ANLN.The TIMER database was used to analyze the correlation between ANLN co-expressed genes and tumor infiltrating immune cells. Results: ANLN is highly expressed in most cancers. In the TCGA-LUAD cohort, ANLN was highly expressed in LUAD. ANLN was significantly related to the grade , T stage and N stage of patients with LUAD. It was verified in multiple independent queues. The high expression and highly mutation of ANLN predicts poor survival .Multivariate COX analysis showed that ANLN was an independent risk factor for survival . GSEA analysis shows that ANLN regulates cell cycle, and other pathways. The expression of ANLN was significantly correlated with the level of B cell infiltration.Conclusions: ANLN is closely related to the prognosis of patients with LUAD and tumor immune cell infiltration suggesting that ANLN is a key factor regulating the recruitment of immune cells to LUAD and may play an important role in immune.
Movement Patterns and Habitat use for Sympatric Species
Authors: McAlpine-Bellis Elizabeth , Utsumi Kaera L , Diamond Kelly M , Klein Janine , Gilbert-Smith Sophia , Garrison Grace E , Eifler Maria A , Eifler Douglas A 
Published: August 2021

Abstract
Background: Movement is an important characteristic of an animal’s ecology, reflecting perception of and response to environmental conditions. To effectively search for food, movement patterns likely depend on habitat characteristics and the sensory systems used to find prey. We examined movements associated with foraging for two sympatric species of lizards inhabiting the Alvord Basin in the Great Basin Desert of southeastern Oregon. The two species have largely overlapping diets but find prey via different sensory cues, which link to their differing foraging strategies — the long-nosed leopard lizard, Gambelia wislizenii, is a visually-oriented predator, while the western whiptail, Aspidoscelis tigris, relies heavily on chemosensory cues to find prey.Methods: Using detailed focal observations, we characterized the habitat use and movement paths of each species. We placed markers at the location of focal animals every minute for the duration of each 30-min observation. Afterwards, we recorded whether each location was in the open or in vegetation, as well as the movement metrics of step length, path length, net displacement, straightness index, and turn angle, and then made statistical comparisons between the two species.Results: The visual forager spent more time in open areas, moved less frequently over shorter distances, and differed in patterns of plant use compared to the chemosensory forager. Path characteristics of step length and turn angle differed between species.Conclusions: The visual predator moved in a way that was consistent with the notion that they require a clear visual path to stalk prey whereas the movement of the chemosensory predator increased their chances of detecting prey by venturing further into vegetation. Sympatric species can partition limited resources through differences in search behavior and habitat use.
Premedication with fentanyl for less invasive surfactant administration (LISA): A Randomized Controlled Trial
Authors: Sk Habibullah , Saha Bijan , Mukherjee Suchandra , Hazra Avijit 
Published: August 2021

Abstract
Currently, there is no consensus regarding analgesic premedication before the LISA procedure. In this randomized controlled trial, we compared the level of comfort of preterm infants receiving fentanyl as analgesic versus no fentanyl during LISA procedure. We randomized 34 preterm infants of 28+ 0 − 33+ 6 weeks of gestation with RDS within 6 h of birth to receive either fentanyl (1 µg /kg intravenous) or no premedication during LISA procedure. Primary objective was to assess the proportion of infants to be comfortable during the procedure (R-PIPP score < 10) and secondarily complications occurring during the procedure, hemodynamically significant patent ductus arteriosus (hsPDA), intraventricular hemorrhage (IVH) (≥ grade 3), bronchopulmonary dysplasia (BPD) and composite outcome of BPD/mortality. Proportion of infants with a R-PIPP score < 10 during LISA was significantly higher in the fentanyl group (13/17 (76.5%) vs 6/17 (35.3%); p value 0.036). There were no differences in secondary outcome parameters. Conclusion: Low dose fentanyl during LISA procedure resulted in more comfort in preterm infants and without increased complication of both the LISA procedure and fentanyl administration. Further studies are needed to determine the safest and most effective pharmacologic measures to prevent pain and discomfort during LISA. Trial registration: www.ctri.nic.in id CTRI/2020/08/027144, registration date August 14, 2020
Incidence and Risk Factors for Early Postoperative Stiffness after Arthroscopic Rotator Cuff Repair in Patients without Preoperative Stiffness
Authors: Cho Chul-Hyun , Bae Ki-Cheor , Kim Du-Han 
Published: August 2021

Abstract
Purpose The purpose of this study was to investigate the incidence and risk factors of early postoperative stiffness in patients without preoperative stiffness undergoing isolated arthroscopic rotator cuff repair (ARCR). Methods Two hundred seventy-four patients who underwent primary ARCR were included. At 3 months after surgery, criteria for shoulder stiffness was set as follows: 1) passive forward flexion < 120˚, or 2) external rotation at side < 30˚. Patients with preoperative stiffness or who underwent additional procedures were excluded. Patients-related, radiological (muscle atrophy and fatty infiltration), and intraoperative (tear size, repair techniques, number of anchors used, and synovitis scores) risk factors were analyzed. Univariate and multivariate analyses were used to identify risk factors for postoperative stiffness. Results Thirty-nine of 274 patients (14.2%) who underwent ARCR developed postoperative stiffness. Univariate analyses revealed that early postoperative stiffness was significantly associated with diabetes mellitus (p = 0.030). However, radiological and intraoperative factors did not affect postoperative shoulder stiffness (all p > 0.05). Multivariate analyses revealed early postoperative stiffness was significantly associated with diabetes mellitus and timing of rehabilitation (p = 0.024, p = 0.033, respectively).Conclusion The overall incidence of early postoperative stiffness following isolated ARCR in patients without preoperative stiffness was 14.2%. Diabetes mellitus and timing of rehabilitation were independent risk factors for early postoperative stiffness following ARCR.
Determinants of Modern Contraception use Among Reproductive Age Women in Cameroon.
Authors: Toguem Michael guy 
Published: August 2021

Abstract
Background: In Cameroon, 23% of married women, 34% of sexually active single women have unmet needs for family planning and 58% of reproductive age women are not interested in family planning. This contributes to the high mother mortality rate, which is at 600 per 100.000 live births. In this study, we tried to identify the factors contributing to the current use of modern contraception by Cameroonian women so that they can be act on to meet the unmet need for modern contraception. Methods: The study uses data from 12,411 women aged from 15 to 49 years old included in the demographic health survey of Cameroon 2018/19. Bivariate, then multivariate logistic regression analysis were conducted on the study outcome of Modern versus non-modern contraception. Statistical significance was taken at p < 0.05.Results: We found out that 18% of the women in Cameroon use a modern contraception. The main determinants for this were; not working, increasing socioeconomic class, no more wanting children, wanting to delay the next childbirth by at least 2 years, and history of domestic violence. Conclusion: The above factors should be considered in the making of a national contraception improvement plan. Meanwhile, none working women being more likely to use a modern contraception should be considered with some reserve because this is a highly unexpected finding.
Baseline Study for Improving Diagnostic Stewardship at Secondary Health Care Facilities in Nigeria
Authors: Egwuenu Abiodun , Ejikeme Adaora , Tomczyk Sara , Von-Laer Anja , Ayobami Olaniyi , Odebajo Oluwaseun , Akhibi Samuel , Agulanna Constance , Inweregbu Stella , Yahaya Ridwan , Okwor Tochi , Dada-Adegbola Hannah , Ajayi Ikeoluwapo , Olorukooba Abdulhakeem , Eckmanns Tim , Ochu Chinwe , Ihekweazu Chikwe 
Published: August 2021

Abstract
BackgroundBlood culture diagnostics are a critical tool for sepsis management and antimicrobial resistance (AMR) surveillance. A baseline study was conducted to assess existing sepsis case finding, blood culture diagnostics and antimicrobial susceptibility testing (AST) at secondary health care facilities to inform the development of diagnostic stewardship improvement strategies in Nigeria.MethodsA cross-sectional online survey was conducted among 25 public secondary health care facilities in Abuja, FCT and Lagos State in Nigeria to evaluate the capacity for pathogen identification and AST. Following this, data were prospectively extracted on all patients with suspected sepsis from electronic medical records at two facilities in Abuja from October 2020 to May 2021 to further assess practices concerning sepsis case-finding, clinical examination findings, samples requested, and laboratory test results. Data were descriptively analysed, and a multivariate logistic regression analysis was conducted to determine factors associated with blood culture requests among suspected sepsis patients.ResultsIn the online survey, 32% (8/25) of facilities reported performing routine blood cultures. Only one had access to a clinical microbiologist, and 28% (7/25) and 4% (1/25) used standard bacterial organisms for quality control of media and quality control strains for AST, respectively. At the two facilities where data abstraction was performed, 7.1% (2924/41066) of patients were found to have suspected sepsis. A majority of the suspected sepsis patients came from the paediatrics department and were outpatients, and the median age was two years. Most did not have vital signs and major foci of infection documented. Blood cultures were only requested for 2.7% (80/2924) of patients, of which twelve were positive for bacteria, mainly Staphylococcus aureus. No clinical breakpoints were used for AST. Inpatients (adjusted odds ratio [aOR]:7.5, 95% CI:4.6 – 12.3) and patients from the urban health care facility (aOR:16.9, 95% CI:8.1 – 41.4) were significantly more likely to have a blood culture requested.ConclusionLow blood culture utilisation remains a key challenge in Nigeria. This has implications for patient care. AMR surveillance and antibiotics use. Diagnostic stewardship strategies should focus on improving access to clinical microbiology expertise, practical guidance on sepsis case finding and improving blood culture utilisation and diagnostics.
COVID-19 in Iran: Clinical Presentations and Outcomes in Three Different Surges of COVID-19 Infection
Authors: Hadadi Azar , Pirzadeh Marzieh , Kazemian Sina , Ashraf Haleh , Ebrahimi Mehdi , Saleh Shahrokh Karbalai , Talebpour Mohammad 
Published: August 2021

Abstract
Background: A few studies compared the characteristics and outcomes of COVID-19 patients during the first and second surges of the disease.We aimed to describe the clinical features and outcomes of COVID-19 patients across the first, second, and third surges of the disease in Tehran, Iran.Method: We conducted aretro-respective cohort study of patients with COVID-19 admitted to Sina hospital of Tehran, Iran,during three surges of COVID-19 from February 16 to October 28, 2020.Result: Surge 1 patients were youngerwith more prevalence of hypertension.They also presented with significantly higher oxygen saturation, systolic blood pressure, andrespiratory rateon admission. Patients had higher levels of neutrophil to lymphocyte ratio, Urea, CRP, and ESR, in surge 2. The incidence of dyspnea, chest pain, and neurological manifestations followed a significant increasing trend from surge 1 to surge 3. There was no difference in severity and in-hospital mortality between the surges. However, the length of hospital stays and acute cardiac injury (ACI) was less in surge 1 and acute respiratory distress syndrome (ARDS) in surge 2 than in other surges. Conclusion: patients did not significantly differ in disease severity, ICU admission, and mortality between surges; however, length of hospital stay and ACI increased during surges, and the number of patients developing ARDS was significantly less in surge 2 compared to other peaks.
First Observations of Plasmodium Malariae and Its Evolution During the Parasitological Evaluation of the Long-term Village Scale Malaria Vector Control Program in the Balombo Area of Angola
Authors: Manguin Sylvie , Foumane Vincent , Toto Jean-Claude , Martinaud Franck , Santos Maria Adelaide Dos , Carnevale Pierre 
Published: August 2021

Abstract
Background: A long-term village scale vector control program was implemented in eight villages around the Balombo town (Benguela Province, Angola) to compare the efficacy of four methods of vector control inside houses including long-lasting deltamethrin insecticide treated nets (PermaNet© 2.0 model or P.2.0); association of P.2.0 and deltamethrin Insecticide Treated Plastic Sheeting (ITPS)-Zero Fly© model; deltamethrin ITPS-Wall Lining model alone; and 2 rounds of lambdacyhalothrin Inside Residual Spraying followed by installation of ITPS.Methods: Cross sectional parasitological surveys (CSS) were done every two months. Thick blood films (TBF) were microscopically examined at the Medical Department of the Angolese Sonamet company in Lobito and a sample of them were double-checked in Yaoundé. Plasmodium species determination, parasitaemia and gametocytes presence, and evolution in time were analyzed.Results: A total of 190 CSS was done between 2007 and 2011, Plasmodium spp. were observed in 5,431 of the 21,804 TBF done. Plasmodium malariae alone was observed in 22 TBF (0.4%) and mixed infections P. falciparum and P. malariae in 44 TBF (0.8%).Conclusion: The very low frequency of P. malariae could explain the fact it is usually not reported in classical point prevalence surveys. Our study confirms the presence of this species in Angola, which must be known due to its special clinical impact, quartan fever, kidney failure, chronicity, symptomless carriers, persistence for several years with long term recrudescence and reported cases of resistance to classical ACTs. The prevalence of P. malariae decreased after implementation of vector control methods.
Facile Preparation of Hierarchically Porous Aromatic-cyclodextrin Polymers and Their Application for the Selective Adsorption of Cationic Dyes
Authors: Duan Huiling , Ma Xiang-Ai , Wang Yu , Cheng Kai-Qi , Zhang Li-Juan , Qi Ji-Chao 
Published: August 2021

Abstract
Porous β-cyclodextrin-containing materials have significant potential as adsorbents for the removal of pollutants from water. However, preparing these porous polymers in the aqueous phase is challenging. In this study, a kind of novel porous aromatic-cyclodextrin polymers (P-aro-CDPs) was designed and synthesized in aqueous solution under mild conditions by exploiting covalence-crosslinking reaction. P-aro-CDPs were characterized using a variety of methods, which revealed that P-aro-CDPs have a hierarchical porous structure, a highly negatively charged surface, and rich in hydroxyl groups. The prepared P-aro-CDPs showed excellent removal efficiency for methylene blue, with a maximum adsorption capacity of 194.17 mg g-1. The adsorption data are well fitted to the pseudo-second-order kinetic model and the Langmuir isotherm. The as-synthesized P-aro-CDPs material exhibited superior adsorption selection toward cationic dyes than anionic dyes whether in single or multicomponent systems. Further, the P-aro-CDPs adsorbent are reusable, and good performance over six adsorption–desorption cycles was demonstrated. Due to its off-the-beaten-path synthesis, low cost, excellent removal efficiency, and recyclability, P-aro-CDPs have great potential for use as an adsorbent in water-treatment applications.
High Expression of NREP Correlated With Worse Survival and M2 Macrophage Infiltrates in Gastric Cancer
Authors: Lu Tailiang , Li Chenlong , Peng Wei , Xiang Cailing , Gong Yongqiang , Chen Chaowu 
Published: August 2021

Abstract
Background: Neuronal Regeneration Related Protein (NREP) is a highly conserved protein and is a newly discovered protein that may be closely related to tumor cell migration. We aim at investigating the prognostic role of NREP in gastric cancer (GC). Methods: Tumor Immune Estimation Resource (TIMER), The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) databases were used to analysis NREP mRNA expression in GC. Correlations between NREP mRNA expression and clinicopathological characteristic were analyzed. TCGA and GEO data were analyzed by The Gene Expression Profiling Interactive Analysis (GEPIA) and Kaplan–Meier plotter databases respectively to assess prognostic value of NREP in GC. Gene set enrichment analysis (GSEA) was performed to identify the keg pathways related to NREP expression. TIMER and CIBERSORT analysis were used to found tumor-infiltrating immune cells associated with NREP mRNA expression. Results: NREP was over expressed in GC and significantly associated with T stage (P<0.001), Histologic grade (P = 0.022) and OS events (P = 0.007) of GC patients. High mRNA expression of NREP correlated with worse survival. The significantly kegg pathways enriched in samples with NREP high expression involved in cell adhesion, tumorigenesis, and immune and inflammatory responses. NREP mRNA expression was positively associated with CD4+T cell(r = 0.294, P = 1.04e−08), CD8+T cell(r = 0.125, P = 0.0167), Neutrophil(r = 0.169, P = 0.00116), Dendritic(r = 0.314, P = 1.03e−09), and was strongly associated with Macrophage (r = 0.547, P = 5.44e−30). The CIBERSORT database revealed that NREP mRNA expression was correlated with the activated memory CD4+ T cells(p<0.01), Monocytes(p<0.001) and M2 Macrophages(p<0.001). NREP is strongly correlated with the markers genes of M2 macrophages and tumor-associated macrophages (TAMs). Conclusion: NREP might be served as a novel prognostic biomarker of GC and associated with M2 macrophage infiltrates.
Long Non-Coding RNA (lncRNA) Sequences: FAM3D-AS1, LINC01230, LINC01315 and LINC01468 – Are They Associated with Endometrial Cancer?
Authors: Bieńkiewicz Jan , Romanowicz Hanna , Szymańska Bożena , Domańska-Senderowska Daria , Wilczyński Miłosz , Stepowicz Anna , Malinowski Andrzej , Smolarz Beata 
Published: August 2021

Abstract
Background: The analysis of long non-coding RNA (lncRNA) in endometrial cancer is quite a novel field of science. Although numerous lncRNA sequences have been identified up until today, their correlation with endometrial cancer is still undetermined. The aim of this study was to analyse the expression of four lncRNA sequences: FAM3D-AS1, LINC01230, LINC01315 and LINC01468 and to investigate their significance in the risk of developing endometrial cancer.Methods: LncRNA sequences have been investigated in paraffin embedded tissue blocks (archival postoperative specimens) in endometrial cancer patients (Cases, n=120) and in cancer-free controls (n=80) using real-time PCR assay.Results: This study revealed a lower expression of LINC01468 in endometrial cancer patients than in controls. Both LINC01468 and FAM3D-AS1 were positively correlated with Body Mass Index (BMI) in cancer-free controls.Conclusions: LncRNA LINC01468 may be a protective factor in development of endometrial cancer.