bluuebunny/crossref_metadata_2025_split huggingface dataset:
Quantitative Proteomic Analysis of Plasma Exosomes to Identify the Candidate Biomarker of Imatinib Resistance in Chronic Myeloid Leukemia Patients
Authors: Li Mei-Yong , Zhao Cui , Chen Lian , Yao Fang-Yi , Zhong Fang-Min , Chen Ying , Xu Shuai , Jiang Jun-Yao , Yang Yu-Lin , Min Qing-Hua , Lin Jin , Zhang Hai-Bin , Liu Jing , Wang Xiao-Zhong , Huang Bo 
Published: December 2021

BackgroundImatinib (IM), a tyrosine kinase inhibitor (TKI), has markedly improved the survival and life quality of chronic myeloid leukemia (CML) patients. However, the lack of specific biomarkers for IM resistance remains a serious clinical challenge. Recently, growing evidence has suggested that exosome-harbored proteins were involved in tumor drug resistance and could be novel biomarkers for the diagnosis and drug sensitivity prediction of cancer. Therefore, we aimed to investigate the proteomic profile of plasma exosomes derived from CML patients to identify ideal biomarkers for IM resistance.MethodsWe extracted exosomes from pooled plasma samples of 9 imatinib-resistant CML patients and 9 imatinib-sensitive CML patients by ultracentrifugation. Then, we identified the expression levels of exosomal proteins by liquid chromatography-tandem mass spectrometry (LC-MS/MS) based label free quantification. Bioinformatics analyses were used to analyze the proteomic data. Finally, the western blot (WB) and parallel reaction monitoring (PRM) analyses were applied to validate the candidate proteins.ResultsA total of 2812 proteins were identified in plasma exosomes from imatinib-resistant and imatinib-sensitive CML patients, including 279 differentially expressed proteins (DEPs) with restricted criteria (fold change≥1.5 or ≤0.667, p&lt;0.05). Compared with imatinib-sensitive CML patients, 151 proteins were up-regulated and 128 proteins were down-regulated. Bioinformatics analyses revealed that the main function of the upregulated proteins was regulation of protein synthesis, while the downregulated proteins were mainly involved in lipid metabolism. The top 20 hub genes were obtained using STRING and Cytoscape, most of which were components of ribosomes. Moreover, we found that RPL13 and RPL14 exhibited exceptional upregulation in imatinib-resistant CML patients, which were further confirmed by PRM and WB.ConclusionProteomic analysis of plasma exosomes provides new ideas and important information for the study of IM resistance in CML. Especially the exosomal proteins (RPL13 and RPL14), which may have great potential as biomarkers of IM resistance.
Tumor Microenvironment Modifications Recorded With IVIM Perfusion Analysis and DCE-MRI After Neoadjuvant Radiotherapy: A Preclinical Study
Authors: Lallemand François , Leroi Natacha , Blacher Silvia , Bahri Mohamed Ali , Balteau Evelyne , Coucke Philippe , Noël Agnès , Plenevaux Alain , Martinive Philippe 
Published: December 2021

PurposeNeoadjuvant radiotherapy (NeoRT) improves tumor local control and facilitates tumor resection in many cancers. Some clinical studies demonstrated that both timing of surgery and RT schedule influence tumor dissemination, and subsequently patient overall survival. Previously, we developed a pre-clinical model demonstrating the impact of NeoRT schedule and timing of surgery on metastatic spreading. We report on the impact of NeoRT on tumor microenvironment by MRI.MethodsAccording to our NeoRT model, MDA-MB 231 cells were implanted in the flank of SCID mice. Tumors were locally irradiated (PXI X-Rad SmART) with 2x5Gy and then surgically removed at different time points after RT. Diffusion-weighted (DW) and Dynamic contrast enhancement (DCE) MRI images were acquired before RT and every 2 days between RT and surgery. IntraVoxel Incoherent Motion (IVIM) analysis was used to obtain information on intravascular diffusion, related to perfusion (F: perfusion factor) and subsequently tumor vessels perfusion. For DCE-MRI, we performed semi-quantitative analyses.ResultsWith this experimental model, a significant and transient increase of the perfusion factor F [50% of the basal value (n=16, p&lt;0.005)] was observed on day 6 after irradiation as well as a significant increase of the WashinSlope with DCE-MRI at day 6 (n=13, p&lt;0.05). Using immunohistochemistry, a significant increase of perfused vessels was highlighted, corresponding to the increase of perfusion in MRI at this same time point. Moreover, Tumor surgical resection during this peak of vascularization results in an increase of metastasis burden (n=10, p&lt;0.05).ConclusionSignificant differences in perfusion-related parameters (F and WashinSlope) were observed on day 6 in a neoadjuvant radiotherapy model using SCID mice. These modifications are correlated with an increase of perfused vessels in histological analysis and also with an increase of metastasis spreading after the surgical procedure. This experimental observation could potentially result in a way to personalize treatment, by modulating the time of surgery guided on MRI functional data, especially tumor perfusion.
Parental Feeding Styles and Their Association With Complementary Feeding Practices and Growth in Mexican Children
Authors: Kim-Herrera Edith Y. , Ramírez-Silva Ivonne , Rodríguez-Oliveros Guadalupe , Ortiz-Panozo Eduardo , Sánchez-Estrada Marcela , Rivera-Pasquel Marta , Pérez-Escamilla Rafael , Rivera-Dommarco Juan Angel 
Published: December 2021

Background: Complementary feeding practices and corresponding parental feeding styles influence nutritional status in later stages of childhood. Findings on the association of these variables with infant growth remain inconsistent; in Mexico, a research gap exists in this area.Research Aims: (1) To characterize parental feeding styles and complementary feeding practices, and (2) to evaluate the association of parental feeding styles with complementary feeding practices and infant growth at 6 and 9 months of age.Methods: Data were collected from a prospective Mexican birth cohort. Parental feeding styles, complementary feeding practices, and anthropometric data from 263 to 234 mother-child pairs (infants of 6 and 9 months of age, respectively) were analyzed. Logistic and linear regression models were used to determine the associations between variables.Results: The predominant parental feeding style was the “responsive style” (90%). Only 43.7 and 8.1% of 6- and 9-month-old infants, had adequate complementary feeding practices, respectively. At 6 months, mothers who were responsive to satiety signals had 11% lesser possibilities (OR = 0.89, 95% CI [0.80, 0.98]) of their infant having inadequate complementary feeding practices than their counterparts and “pressuring to finish” and “pressuring to eat cereal” sub-constructs were associated with lower weight for length and body mass index Z-scores (p = 0.02).Conclusions: A high proportion of infants (&gt;40%) did not meet international recommendations. The “pressuring” parental feeding style sub-constructs were associated with growth indicators in 6-month old infants. This emphasizes the importance of promoting parental responsiveness to infant appetite and satiety signals to achieving adequate complementary feeding practices.
Key Phytochemicals and Biological Functions of Chuanxiong Rhizoma Against Ischemic Stroke: A Network Pharmacology and Experimental Assessment
Authors: Zeng Peng , Yi Yao , Su Hong-Fei , Ye Chao-Yuan , Sun Yi-Wen , Zhou Xin-Wen , Lu Youming , Shi Anbing , Tian Qing 
Published: December 2021

Presently, the treatment options for ischemic stroke (IS) are limited due to the complicated pathological process of the disease. Chuanxiong Rhizome (CR), also known as Conioselinum anthriscoides “Chuanxiong” (rhizome), is the most widely used traditional Chinese medicine for treating stroke. This study aimed to uncover the key phytochemicals and biological functions of CR against IS through a network pharmacology approach combining with IS pathophysiology analysis. We employed permanent unilateral common carotid artery ligation to construct a mouse model of global cerebral ischemia and found that cerebral ischemia injuries were improved after 7 days of gavage treatment of CR (1,300 mg/kg/day). CR exerts protective effects on neurons mainly by acting on targets related to synaptic structure, synaptic function, neuronal survival and neuronal growth. A total of 18 phytochemicals from CR based on UHPLC-MS/MS that corresponded to 85 anti-IS targets. Coniferyl ferulate, neocnidilide and ferulic acid were identified as the key phytochemicals of CR against IS. Its brain protective effects involve anti-inflammatory, anti-oxidative stress, and anti-cell death activities and improves blood circulation. Additionally, the two most important synergistic effects of CR phytochemicals in treating IS are prevention of infection and regulation of blood pressure. In brain samples of Sham mice, L-tryptophan and vanillin were detected, while L-tryptophan, gallic acid, vanillin and cryptochlorogenic acid were detected in IS mice by UHPLC–MS/MS. Our findings provide a pathophysiology relevant pharmacological basis for further researches on IS therapeutic drugs.
Empagliflozin Reduces Renal Hyperfiltration in Response to Uninephrectomy, but Is Not Nephroprotective in UNx/DOCA/Salt Mouse Models
Authors: Tauber Philipp , Sinha Frederick , Berger Raffaela S. , Gronwald Wolfram , Dettmer Katja , Kuhn Michaela , Trum Maximilian , Maier Lars S. , Wagner Stefan , Schweda Frank 
Published: December 2021

Large-scale clinical outcome studies demonstrated the efficacy of SGLT2 inhibitors in patients with type II diabetes. Besides their therapeutic efficacy in diabetes, significant renoprotection was observed in non-diabetic patients with chronic kidney disease (CKD), suggesting the existence of glucose-independent beneficial effects of SGLT2 inhibitors. However, the relevant mechanisms by which SGLT2 inhibition delays the progression of renal injury are still largely unknown and speculative. Previous studies showed that SGLT2 inhibitors reduce diabetic hyperfiltration, which is likely a key element in renoprotection. In line with this hypothesis, this study aimed to investigate the nephroprotective effects of the SGLT2 inhibitor empagliflozin (EMPA) in different mouse models with non-diabetic hyperfiltration and progressing CKD to identify the underlying diabetes-independent cellular mechanisms. Non-diabetic hyperfiltration was induced by unilateral nephrectomy (UNx). Since UNx alone does not result in renal damage, renal disease models with varying degrees of glomerular damage and albuminuria were generated by combining UNx with high NaCl diets ± deoxycorticosterone acetate (DOCA) in different mouse strains with and without genetic predisposition for glomerular injury. Renal parameters (GFR, albuminuria, urine volume) were monitored for 4–6 weeks. Application of EMPA via the drinking water resulted in sufficient EMPA plasma concentration and caused glucosuria, diuresis and in some models renal hypertrophy. EMPA had no effect on GFR in untreated wildtype animals, but significantly reduced hyperfiltration after UNx by 36%. In contrast, EMPA did not reduce UNx induced hyperfiltration in any of our kidney disease models, regardless of their degree of glomerular damage caused by DOCA/salt treatment. Consistent with the lack of reduction in glomerular hyperfiltration, EMPA-treated animals developed albuminuria and renal fibrosis to a similar extent as H2O control animals. Taken together, the data clearly indicate that blockade of SGLT2 has the potential to reduce non-diabetic hyperfiltration in otherwise untreated mice. However, no effects on hyperfiltration or progression of renal injury were observed in hypervolemic kidney disease models, suggesting that high salt intake and extracellular volume might attenuate the protective effects of SGLT2 blockers.
Music Therapy vs. Music Listening for Negative Symptoms in Schizophrenia: Randomized, Controlled, Assessor- and Patient-Blinded Trial
Authors: Pedersen Inge Nygaard , Bonde Lars Ole , Hannibal Niels Jørgensen , Nielsen Jimmi , Aagaard Jørgen , Gold Christian , Rye Bertelsen Lars , Jensen Silvia Beatriz , Nielsen René Ernst 
Published: December 2021

Objective: To investigate the efficacy of music therapy for negative symptoms in patients with schizophrenia.Methods: Randomized, participant- and assessor-blinded, multicenter, controlled trial including patients diagnosed with schizophrenia according to ICD-10 with predominantly negative symptoms, between 18 and 65 years. Participants were randomized to 25 successive weekly individual sessions (excluding holidays, including cancellation by the participant) of either music therapy conducted by trained music therapists, or music listening together with a social care worker. The primary outcome was reduction in negative symptoms as measured by The Positive and negative Syndrome Scale (PANSS) negative subscale total score, assessed by a blinded rater, utilizing mixed-effects model analysis.Results: In total, 57 participants were randomized; 39 completed the study's initial 15 weeks, and 30 completed follow-up at 25 weeks. On the primary outcome of PANSS negative subscale, no significant difference was observed between groups with a coefficient of −0.24 (95% CI −1.76 to 1.27, P = 0.754) in the intention to treat analysis, and −0.98 (95% CI −5.06 to 3.09, P = 0.625) when only analyzing completers. Both interventions showed significant reduction from baseline to 25 weeks on PANSS negative subscale. On secondary outcomes, no between group differences were observed in The Brief Negative Symptom Scale, WHOQOL-Bref (Quality of Life), The Helping Alliance Questionnaire and The Global Assessment of Functioning in the intention to treat or completers populations utilizing Mixed Effects Models.Conclusion: No difference between groups randomized to music therapy vs. musical listening was observed resulting in no clear recommendation for which intervention to use as the first choice for treatment of patients diagnosed with schizophrenia and predominantly having negative symptoms.Clinical Trial Registration:www.ClinicalTrials.gov, identifier: NCT02942459.
Development of an Online Resource for People Bereaved by Suicide: A Mixed-Method User-Centered Study Protocol
Authors: Leaune Edouard , Lestienne Laurène , Grandgenèvre Pierre , Morgiève Margot , Vaiva Guillaume , Vieux Maxime , Chalancon Benoît , Laplace Nathalie , Haesebaert Julie , Poulet Emmanuel 
Published: December 2021

Introduction: Suicide bereavement is known to be highly distressing and is frequently associated with mental health problems. Despite high-level of need regarding mental and physical health, people bereaved by suicide display low level of help-seeking and perceived support in the aftermath of the loss. The lack of accessibility and reliability of face-to-face counseling resources is notably reported by suicide survivors. Online resources can enhance early access to help and support for people bereaved by suicide. The primary objective of the study is to design and implement an innovative and adaptive online resource for people bereaved by suicide according to their needs and expectation regarding online solutions dedicated to suicide bereavement.Methods: The ESPOIR2S study is a mixed-method user-centered study. ESPOIR2S seeks to build the resource from the perspectives and needs of both people bereaved by suicide and professionals or volunteers working in the field of postvention. The Information System Research (ISR) Framework is used to guide the design of the study through a 3-step research cycle. The structure of the ESPOIR2S study relies on a simultaneous collection of qualitative and quantitative data which will be collected and analyzed during (a) the Relevance cycle through an online questionnaire and focus groups; (b) the Design cycle through focus groups; and (c) and the Rigor cycle through an online questionnaire and semi-structured interviews. The user-centeredness will be ensured by the active participation of people bereaved by suicide, members of associations for bereaved people and professionals of postvention.Discussion: The mixed-method and user-centered design of the ESPOIR2S study will offer an in-depth collection of the needs and expectation of suicide survivors regarding online resources. Through the implementation of an adaptive online solution, we aim to enhance the access to help and support for suicide survivors which are highly correlated with well-being and recovery.
Drone Surveying of Volumetric Ice Growth in a Steep River
Authors: Rødtang Einar , Alfredsen Knut , Juárez Ana 
Published: December 2021

Representative ice thickness data is essential for accurate hydraulic modelling, assessing the potential for ice induced floods, understanding environmental conditions during winter and estimation of ice-run forces. Steep rivers exhibit complex freeze-up behaviour combining formation of columnar ice with successions of anchor ice dams to build a complete ice cover, resulting in an ice cover with complex geometry. For such ice covers traditional single point measurements are unrepresentative. Gathering sufficiently distributed measurements for representativeness is labour intensive and at times impossible with hard to access ice. Structure from Motion (SfM) software and low-cost drones have enabled river ice mapping without the need to directly access the ice, thereby reducing both the workload and the potential danger in accessing the ice. In this paper we show how drone-based photography can be used to efficiently survey river ice and how these photographic surveys can be processed into digital elevation models (DEMs) using Structure from Motion. We also show how DEMs of the riverbed, riverbanks and ice conditions can be used to deduce ice volume and ice thickness distributions. A QGIS plugin has been implemented to automate these tasks. These techniques are demonstrated with a survey of a stretch of the river Sokna in Trøndelag, Norway. The survey was carried out during the winter 2020–2021 at various stages of freeze-up using a simple quadcopter with camera. The 500 m stretch of river studied was estimated to have an ice volume of up to 8.6 × 103 m3 (This corresponds to an average ice thickness of ∼67 cm) during the full ice cover condition of which up to 7.2 × 103 m3 (This corresponds to an average ice thickness of ∼57 cm) could be anchor ice. Ground Control Points were measured with an RTK-GPS and used to determine that the accuracy of these ice surface geometry measurements lie between 0.03 and 0.09 m. The ice thicknesses estimated through the SfM methods are on average 18 cm thicker than the manual measurements. Primarily due to the SfM methods inability to detect suspended ice covers. This paper highlights the need to develop better ways of estimating the volume of air beneath suspended ice covers.
Investigations of Thermal Stability and Spectroscopic Features of Sm3+ Doped Strontium Aluminate Glasses
Authors: Li Pengfei , Zhang Xiaoyan , Zhang Jinrong , Qi Xiwei , Liu Xin 
Published: December 2021

In the present work, a series of Sm3+ doped transparent strontium aluminate glasses with the composition Al2O3-(3-x)SrO: xSm3+ (x = 0, 0.01, 0.03, 0.06, 0.1, 0.2) were fabricated by a containerless process using an aerodynamic levitation furnace. The structural characteristics, density, Vicker’s hardness, and thermal and spectroscopic behaviors of these glasses were investigated. All the glasses exhibit excellent thermal stabilities (Tg ≥ 792 °C) and the glass-forming ability is enhanced with the increasing content of Sm3+. The emission spectra recorded under an excitation of 404 nm show four emission transitions as a result of 4G5/2 translated to the lower states of 6H5/2, 6H7/2, 6H9/2, and 6H11/2, and a bright orange-reddish luminescence can be observed in Al2O3-(3-x)SrO: xSm3+ glasses. The high thermal stability, good glass-forming ability and excellent hardness provide new options for the development of visible orange-reddish lasers and smart photoluminescent glass coating materials.
ASPECTS OF USING CYBERSPACE FOR CORPORATE MANAGEMENT SYSTEMS
Authors: ЗАКАЛКИН П.В. 
Published: December 2021

Даны определение термина «киберпространство» и описательное представление киберпространства как пространства. Проведено сравнение свойств физического пространства и киберпространства,а также системы связи и киберпространства. Выделены ключевые отличия киберпространства от систем связи. Рассмотрена роль киберпространства в предоставлении услуг корпоративным системам управления. Показано, что использование результатов исследования в совокупности со структурно-функциональной моделью киберпространства позволит разработать его аналитико-имитационную модель.
A definition of the term "cyberspace" and a descriptive representation of cyberspace as a space are given. A comparison of the properties of physical space and cyberspace, as well as the communication system and cyberspace, is given. Key differences between cyberspace and communication systems are highlighted. The role of cyberspace in providing services to corporate management systems is considered. It is shown that the use of the results of the study in conjunction with the structural and functional model of cyberspace will allow to develop its analytical and simulation model.
CYBERSPACE, INFORMATION SERVICES, COMMUNICATIONS SYSTEM, CORPORATE MANAGEMENT SYSTEM
Broadband, large-numerical-aperture and high-efficiency microwave metalens by using a double-layer transmissive metasurface
Authors: Liu Yong-Qiang , Ren Zhongru , Shu Yingchao , Wu Lujun , Sun Jinhai , Cai He , Zhang Xutao , Lu Lan , Qi Kainan , Li Liangsheng , Che Yongxing , Yin Hongcheng 
Published: December 2021

Abstract
Broadband metalenses consisting of sub-wavelength phase gradient elements are indispensable in modern science and technology. So far, several broadband optical metalenses are demonstrated but mostly with either small NA or relatively low focusing efficiency. Herein, an ultra-thin broadband microwave metalens (frequency range from 8.0 to 10.5 GHz) with a relatively high-efficiency above 40% and a large NA of more than 0.6 is presented. The metalens is also fabricated and the measurement results agree with the simulations very well. The performances of the presented broadband metalens can surpass nowadays microwave metalenses largely and open up new vistas for low-profile, low-cost and light-weight microwave components.
Development of a protocol for genetic transformation of Malus spp
Authors: Martinelli Federico , Perrone Anna , Dandekar Abhaya M. 
Published: December 2021

A protocol to produce transgenic shoots of Malus X domestica cv Greensleaves was optimized using two gene constructs previously used to create parthenocarpic tomato, Ino-IaaM and DefH9-IaaM. The aim was to obtain sufficient nº of transgenic shoots for in vitro multiplication, transfer to soil, grafting and testing for parthenocarpy in the next years. We investigated the effects of two modifications of a previous published protocol: 1) co-transformation with an Agrobacterium containing “VIP” genes in the gene construct and 2) two different hormones or hormone combinations. More shoot regeneration was obtained with a combination of three hormones (BA:NAA:TDZ) during co-cultivation instead of IBA and no co-transformation was performed using the VIP gene. For the DefH9-IaaM transgene, 21.04% regeneration was achieved for this treatment instead of 8.95% achieved with “IBA treatment” and 4.42% with the Agrobacterium co-transformation treatment. More shoot regeneration occurred with the combination of three hormones (BA:NAA:TDZ) instead of with only IBA and no co-transformation was performed using VIP gene. Experiments using Ino-IaaM confirmed the results shown for the DefH9-IaaM transgene. The regenerated shoots were multiplied in selective media containing kanamycin and roots were obtained.