bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification of dwarfism loci Dw1 and Dw2 in clonal apple rootstocks using molecular markers
Authors: Shamshin IN , Dubrovsky M L , Grosheva E V , Trifonova A A , Boris K V 
Published: November 2021

Abstract
The paper presents the results of identification of quantitative trait loci (QTL) of Dw1 and Dw2 involved in the control of dwarf growth in clonal apple rootstocks using the molecular markers. In total, 14 forms of rootstocks were analyzed. The microsatellite markers Hi01c04, Hi04a08, CH03a09 (for the Dw1 locus), MDP0000365711, and MDP0000243703 (for the Dw2 locus) were used in the study. Analysis of the results did not reveal a relationship between the presence of a marker and the manifestation of a sign. Of the three markers of the Dw1 locus, only Hi01c04 amplifies a fragment of the expected size of 120 bp. It was found in 9 forms of rootstocks (PB-4, 57-491, 83-1-15, M9, G16, 2-12-10, 2-9-102, 4-6-5 and 70-20-20). The Hi01c04 marker was identified in both dwarf and medium-sized forms. Of the two markers of the Dw2 locus, the desired fragment is amplified in MDP0000365711. It is typical for almost all genotypes. The exception is 83-1-15, 2-12-10 and 70-20-20, which have a null allele. To assess the genetic diversity of the Dw1 and Dw2 loci, 6 microsatellite sequences Hi01c04, Hi04a08, CH03a09, CH02d08, MDP0000365711, and MDP0000243703 were used. In 14 studied samples, 29 allelic variants ranging in size from 102 bp. up to 170 bp were identified. The number of alleles per locus varied from 1 (for the MDP0000365711 locus) to 7 (for the Hi04a08 locus). No rare alleles were identified. All alleles were observed more than three times. Based on the analysis of SSR spectra, a dendrogram reflecting the similarity of the genotypes under study was built.
Growth features of apple varieties and forms with natural and compact crown types
Authors: Zemisov A S , Youshkov A N , Saveleva N N , Grigoreva L V , Borzykh N V , Khlupova N V , Tarova Z N 
Published: November 2021

Abstract
This research explores the compactness of apple varieties and forms with different types of crown. Orchards on semi-vigorous rootstocks with density and high density planting systems are cost-effective in modern horticulture. For making such gardens, it is necessary to have trees with special compact crown shape. They ensure the high-quality fruits production, meeting the requirements of world standards. To date the most perspective apple varieties and forms for domestic intensive gardening from the rich gene pool of the Selection and Genetic Center of Federal State Scientific Institution «I. V. Michurin Federal Scientific Center» are identified. Varieties of folk domestic and foreign breeding took part in the creation of new genotypes. Apple varieties and promising forms (more than 160 genotypes), including from near and far abroad selection, were used as materials for crown features studying. We conducted our study by the following indicators: tree height, crown volume, “degree of compactness” and the shoot-forming ability. We found that the genotypes Sholokhovskoye, Zvezda Artemyeva, 25-7(11), 40-9(8), 36-8(21), 40-9(6), 8-7(72), 40-9(7), 36-8(17), 62-5(140) have optimal crown parameters. We recommend their use for industrial horticulture and further breeding to create restrained growth, low-volume crown, and a high degree of compactness varieties.
Chemical composition, functional and technological (processing) properties of whey ingredients
Authors: Melnikova E I , Bogdanova E V , Paveleva D A 
Published: November 2021

Abstract
The growth in volumes of the milk whey manufacturing has revealed the new field of processing, such as dry whey ingredients production. The authors have made investigations of chemical composition, functional and technological (processing) properties of whey protein concentrates with protein of 35, 55, 80 % in dry matter, whey protein hydrolysate and cheese whey permeate. We used standard methods, generally accepted in research practice. The chemical composition of the tested samples has been determined, including their rehydration properties in terms of wettability, dispersibility and solubility. Heat denaturation of whey proteins during the processing is the reason for the bound groups–SH release and their reactivity enhancement, which provides antioxidant effect of whey ingredients (the antioxidant content in the tested samples is 0.031; 0.058; 0.095; 0.146 and 0.024 mg/g for the whey protein concentrates with protein of 35, 55, 80 % in dry matter, whey protein hydrolysate and whey permeate respectively). The functional and technological properties of whey ingredients make possible their application while producing different product line groups in order to control the technological processes and the quality factors of the enriched products.
Cultivation method for revitalized potato material in the system of original seed production
Authors: Buldakov S A , Shakleina N A , Plekhanova L P 
Published: November 2021

Abstract
To arrange a system of virus-free potato seed production in the Sakhalin region, a series of studies undertaken to come up with a method towards potato material cultivation in film-gauze greenhouses. At the initial stage, the key agrotechnical methods were developed for growing test-tube microplants, including optimal planting plans – 70×15 cm (the highest yield derived from a standard mini-tuber fraction pcs/m2), 70×30 cm (for clone selection); spraying with insecticides (Aktellik – 3 l/ha, Rogor – 2 l/ha) and fungicides (Ridomil – 1 kg/ha, Tsineb – 2.4 kg/ha) to combat agents transmitting viral and fungal infections; chlorocholine chloride treatment (1.1%) to reduce the growth of the vegetative mass; serological analysis of plants for latent infection with viruses X, Y, S, M, F; burning tops with Reglon (2 l/ha) 2 weeks before harvesting. A set of agrotechnical methods used for producing healthy seed tubers on the Timiryazevskoye seed farm made it possible to reduce the elite cultivation cycle from 5 to 4 years, increase the yield by 57.2%, and reduce the production cost by 43.5%. Subsequently, the methods worked out for producing potato seeds were improved based on modern breeding varieties and phytoregulators (Azolen, Elena, Extrasol, Epin-Extra, Zircon) in spray mixtures with the fungicide Shirlan, contributing to an increased yield up to 34% and efficiency of a standard seed fraction up to 33%. Under production conditions, this method provided a conditionally net income of 450-700 thousand rubles/ha.
The content and characteristics of the accumulation of carotenoids in the grain of modern corn hybrids
Authors: Khoroshilov S A , Voronin A N , Klimenko M N , Biryukova T V , Derevlev E I , Lavrinenko P S 
Published: November 2021

Abstract
The studies were carried out in 2018–2019 in the Belgorod region to identify hybrids and parental forms of corn with increased content of carotenoids in the grain. The material for the study was 4 domestic hybrids, 3 hybrids of the selection of Maizadur, 2 - Syngenta, 1 - Monsanto, 1 - Woodstock, standard - Belkorn 250 MB, as well as 4 parental components of the Decent SV hybrid. During the experiment, agricultural practices traditional for the region were used. The harvesting of the registration plots was carried out manually, considering the weight of the cobs and the harvesting moisture content of the grain. The content of carotenoids in grain was determined according to the developed method in terms of lutein. In 2018, a preliminary screening of a sample of hybrids was carried out. All studied hybrids, except for GIS 1 and GIS 3, surpassed the Belkorn 250 SV standard. The concentration of carotenoids in two domestic hybrids Resource SV (18.4 mg/kg) and Decent SV (19.8 mg/kg) surpassed the hybrids of the Maizadur selection by 35.9 and 40.4% of the Syngenta selection by 8.7 and 15, 2%, Monsanto - by 26.6 and 31.8%, Woodstock - by 8.2 and 14.6%, respectively. On average, over two years, the highest content of carotenoids in grain and their harvest per unit area was observed for the corn hybrid Decent DM (21.8 mg/kg and 169.4 g / ha, respectively), in which the values of these indicators exceeded the Belkorn 250 MV standard in 2 times. Among the parental components, the self-pollinated lines EM 10 3C (26.6 mg/kg) and SB 5-1 SV (29.7 mg/kg) were characterized by a high concentration of carotenoids - the parental components of the Decent SV hybrid.
Assessment of the adaptive potential of strawberry genotypes based on the development of endophytic microbiota and identified R-genes
Authors: Luk’yanchuk IV , Zaitseva K V , Lyzhin A S 
Published: November 2021

Abstract
The paper presents the results of a study of the strawberry genotypes (Fragaria L.) by characteristics of endophytic microbiota and genetic determinants of resistance to fungal pathogens. Highly adaptive genotypes were identified: F. virginiana Duch. ssp. platypetala, F. orientalis Los., F. ovalis Rydb., F. moschata Duch., 298-19-9-43, Urozhaynaya CGL, Flora, Privlekatelnaya and Troitskaya. These strawberry forms are characterized by a high frequency of testing of bacterial microbiota, which suppresses endophytic fungal pathogens. The wild strawberry F. virginiana Duch. ssp. platypetala is characterized by red stele root rot resistance (Rpf1 gene). Strawberry varieties Borovitskaya and Elianny are characterized by anthracnose resistance (Rca2gene).
Effect of granular fertilizer of disinfected chicken dung application on crop productivity and soil fertility
Authors: Stepantsova L V , Matsnev I N , Palchikov E V , Volkov S A , Moskaleva E V 
Published: November 2021

Abstract
On dark gray forest and leached chernozem soils of the Tambov region, the effectiveness of using new granular organic fertilizer from poultry excreta was evaluated. After disinfection of the litter by deep drying, the total content of NPK in the fertilizer constitutes 11-12%, the content of trace elements is about 1g/kg. Field experiments conducted in 2019 showed that the yield increase from the introduction of the studied organic fertilizer at a dose of 4-8 t/ha on spring wheat was 25-30%, on spring barley - 20-23%, on sugar beets from the introduction of 8 t/ha - 10-12%, on potatoes - 40-50%, on tomatoes from 6 t/ha - 30-32% Profitability from the use of this fertilizer on cereals is 130-145%, on technical crops - 140-150%, on vegetables - 230-250%. Optimal fertilizer doses contribute to increasing protein in wheat grains by 1.5%, sugar content in sugar beets by 8-9%, starch content in potatoes by 3-4%, and sugar, dry substances and vitamin C content in tomatoes by 1-2%. The introduction of fertilizer in doses higher than optimal contributes to the increase of basic nutrients and micronutrients content in the soil.
Methods for restoring fertility and improving physical and mechanical properties of soils
Authors: Zavrazhnov A I , Vedishchev S V , Kadomtsev A I , Pavlov A G , Prokhorov A V , Vyguzov M E 
Published: November 2021

Abstract
Insufficient intake of organic matter into the soil, non-compliance with crop rotations result in the soil degradation. Only due to the use of mineral nutrition, it is impossible to maintain a high level of soil fertility. To compensate for the lack of humus, the most optimal is the use of green fertilizers. The costs of producing and applying cover crops are significantly less than the costs of using organic fertilizers. In terms of peasant farms conditions in the Tambov region, two types of crop rotations with the introduction of sideral crops are recommended. It is important to carry out grinding and embedding of sideral crops specifically so that it can provide optimal parameters for the decomposition of organic mass and promote humus formation. Currently, four processing techniques are used for harvesting and embedding sideral crops. The recommended scheme involves mowing and step-by-step grinding of the grass stand with a mower-shredder located in front of the tractor, followed by embedding in the soil with a disc harrow. The analysis shows that small producers segment has no affordable machines for high-quality grinding of sideral crops with low specific energy consumption. To ensure stems cutting with minimal specific energy consumption, a mower has been developed, which, due to the trough-shaped type of its body, forms plants tilted forward in a mass and then, they fall into the grinding zone with the angle of inclination of the stems in this mass close to the angle of body inclination. In the grinding zone, the plant stems are exposed to knives rotating in plane surfaces perpendicular to the longitudinal surface of the mower body. As a result of operational tests of the mower-shredder of sideral crops and crop residues, it was found that technological process of plants grinding is carried out steadily and with a quality that meets agrotechnological requirements.
Efficiency of nutrient solutions with different concentration in the cultivation of white cabbage seedlings
Authors: Blagorodova E N , Varfolomeeva N I , Zvyagina A S , Krasnoselova E A 
Published: November 2021

Abstract
Fertilizers help to fill the missing nutritional elements for plants and affect their growth and the future crop. The paper presents the results of conducted studies on the effect of nutrient solution concentration on morphological and biochemical indicators of white cabbage seedlings. The seedlings of two early-ripe cabbage hybrids – Mirror F1 and Tiara F1 – were grown in peat substrate trays. A nutrient solution of 50 ppm (No.1) and 150 ppm (No.2) was used to feed the seedlings twice during the growing period. The obtained results showed the differences in experimental variants in terms of the height of seedling plants, the number of leaves and leaf-area duration, the weight and volume of the root system, total solids, content of sugar, ascorbic acid. The varietal reaction of cabbage seedlings to the introduction of root feeds with different concentrations was revealed. The nutrient solution No. 2 of hybrid plants Mirror F1 stimulated the development of roots, their weight exceeded the control indicators (without fertilizers) by 37.3%, and the volume – by 37.8%, contributed to the increase of the content of solids (8.27%), general sugar (0.53%), ascorbic acid (78.79 mg%). When fed with nutrient solution No. 2 the seedlings of Tiara F1 hybrid were characterized by a large leaf-area duration, which exceeds the control by 1.3 times, and the indicators of the root system. The plants of this seedlings consisted of 49.5% of the root system. The chemical analysis of water in greenhouse farming for irrigation of plants showed that it contains various macro- and microelements that are necessary for the growth of vegetable seedlings. The concentration of the nutrient solution did not significantly affect the content of macroelements in the seedlings. When using nutrient solution No. 2, an increase in the plants of the two studied phosphorus hybrids was noted, a deficit of which is very often observed when growing seedlings.
The promising variety of European radish Ophelia grown in protected and open ground conditions
Authors: Kosenko M A 
Published: November 2021

Abstract
Among the existing variety of vegetables, table root crops are very popular both in the Russian Federation and abroad: carrots, beets, radishes, turnips and parsnips. These crops are sources of natural vitamins; due to low prices for marketable products and seeds, they have been cultivated in Russia. Among all vegetables, radish ranks first by the content of potassium, magnesium and calcium salts; it also contains iron and phosphorus. When growing new varieties and hybrids of radish suitable for the mechanized harvesting, one should pay attention to the strength of leaves, the erectness of leaf rosettes, the uniformity of immersion in the soil and the easy pull-out of root crops. The collection of root crops Raphanus sativus L. VIR is annually replenished with numerous samples of the latest selection, primarily from China, Japan, and the Netherlands, as well as samples collected in Central Asia and the Caucasus. The main task of greenhouse vegetable growing is year-round or off-season production of high-quality vegetables (daikon, radish and turnip). As a result of the research, a new variety of European summer radish Ophelia was created for growing in protected and open ground conditions. It is an early ripening variety: it takes 33-38 days from full germination to the beginning of economic ripeness. Leaf rosettes are of medium size, light green. The vegetable has white elliptical roots. The Base is rounded. The pulp is white and opaque
The quality of spring wheat and barley grain under the influence of protective-stimulating preparations in the conditions of the forest-steppe zone of the Trans-Urals
Authors: Moiseeva K V , Shulepova O V 
Published: November 2021

Abstract
The purpose of the research is to study the effect of the fungicide Lamador and the humic preparation Rostok on the quality of spring wheat and spring barley in the conditions of the northern forest-steppe of the Tyumen region. According to the yield, we found that pre-sowing seeds treatment with Lamador protectant and joint treatment with Rostock contributed to an increase in yield by 0.70 t/ha of spring wheat and 0.15 t/ha of spring barley, in the option with the pre-sowing seeds treatment with Lamador protectant and Rostock preparation by 0.90 t/ha and 0.10 t/ha. The highest yield of spring wheat was noted in the third option – 4.10 t/ha, of spring barley in the second option – 3.52 t/ha. An increase in the thousand kernel weight was noted in the crops under study. The highest thousand kernel weight was observed for spring wheat and spring barley in the third option – 30.2 g and 54.3 g, respectively. The maximum protein content in the spring wheat grain was noted in the third option –13.3%, which is higher than the control option by 1.3%, for spring barley we also noted protein increase by 0.4-0.5%. Thus, the experimental application of the fungicide Lamador together with the preparation Rostok for wheat seeds treatment demonstrated a positive effect of the protectants on plants in the agro-climatic conditions of the forest-steppe zone of the Tyumen region and contributed to an increase in crop yield by 28% for spring wheat and 4% for spring barley, which proved the need for their use in the plant protection system, to increase the productivity and quality of the grain obtained.
Influence of rootstock on the quality of sweet cherry fruits in the conditions of the Lower Volga region
Authors: Nikolskay OA , Solonkin A V , Kikteva E N 
Published: November 2021

Abstract
Sweet cherry is a thermophilic and whimsical crop that reacts painfully to unfavorable meteorological conditions, such as frost, drought or high humidity in the air and soil. At the same time, this crop requires a uniform supply of heat and moisture during the growing season. In the climatic conditions of the Volgograd region, stressful situations often arise that can partially or completely reduce the yield of fruit crops, especially those introduced to our region from other regions. To reduce the risk of losing the yield of fruit plantations, including sweet cherry, it is necessary to pay special attention to the choice of the variety, as well as the rootstock on which this variety is grafted. The publication presents the results of studying the influence of rootstocks on the qualitative and quantitative indicators of sweet cherry fruits, such as taste, weight, uniformity, color, and productivity. According to the results of the study in the field (in the garden), varietal-rootstock combinations were identified that have one-dimensional, crack-resistant, large fruits, while maintaining high and stable productivity. According to the results of research, it was found that the rootstock affects not only the strength of growth and the shape of the crown, but also the quality of the fruit. The most dependent of the studied varieties on the type of rootstock is Euphoria, the lowest weight of berries was observed on the rootstock VSL-1. At the same time, this rootstock has a higher percentage of resistance to cracking of the fruit. Based on the conducted research, it can be concluded that with the help of rootstock, it becomes possible to influence the quality indicators of fruits.
The combining ability of self-pollinated lines of the red beet(Beta vulgaris L)
Authors: Timakova L N , Khovrin A N , Dolgopolova M A 
Published: November 2021

Abstract
The proper choice of starting material determines the results of the selection. If the starting forms of plants are not fit for crossing and do not match the goals set or the soil and climate conditions available, any selection work may prove futile. The creation of new starting material is based on the study of the existing varieties and the selection of the best biotypes, as well as the assessment of a set of biological and economically valuable parameters of breeding. Inbreeding is the most widely used method of obtaining new starting material, especially homozygous lines, and it plays a key role in heterosis selection programs for the majority of agricultural crops. The use of beet heterosis allows for a significant increase in homogeneity, quality, and marketability of the produce. In 2018-2020, nine samples of red beet starting material were assessed. Using the polycross methods, we assessed the general combining ability of self-pollinated red beet lines across the parameters determining the commercial properties of this root crop: the proportion of the root crop top in its diameter, the share of leaves in the plant biomass, and regularity of shape and marketability. The subject matter of this work is the lines of red beet with round root crops. Research material includes the raw data from field measurements and records. We used the Kestrel F1 hybrid variety released for Central Russia. Field tests were carried out using the uniform procedures set out by the All-Russian Horticultural Research Institute - a branch of the Federal Research Center for Horticulture from Moscow Oblast. At the experimental locations, we used the agricultural practices typical of the region. Using the properties - the small proportion of the root crop top (42.1-47%) and the share of leaves in the biomass (24.1-27.5%), high regularity of root crop shape (up to 85.6%), and marketability (88.3-95.9%) - we determined the following lines: 506 I2, 507 I2.
Puree from apple and carrot refuse, boiled with starch syrup, in marshmallow technology
Authors: Perfilova OV , Blinnikova O M , Suhareva T N , Popova E I , Bryksina K V 
Published: November 2021

Abstract
For modern society, diseases associated with malnutrition are increasingly common. There is an urgent task of saturating the consumer market with products for a healthy diet, which are balanced in nutritional substances and contain essential nutrients, including confectionery. The article proves that in the production of marshmallows for solving this problem, it is possible to successfully use a puree from apple and carrot refuse, boiled with starch syrup. This technological solution improves organoleptic quality indicators and increases the content of flavonoids, beta-carotene and dietary fiber in the finished product.