bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evolutionary priming and transition to the ectomycorrhizal habit in an iconic lineage of mushroom-forming fungi: is preadaptation a requirement?
Authors: Looney Brian , Miyauchi Shingo , Morin Emmanuelle , Drula Elodie , Courty Pierre Emmanuel , Kohler Annegret , Kuo Alan , LaButti Kurt , Pangilinan Jasmyn , Lipzen Anna , Riley Robert , Andreopoulos William , He Guifen , Johnson Jenifer , Nolan Matt , Tritt Andrew , Barry Kerrie W. , Grigoriev Igor V. , Nagy László G. , Hibbett David , Henrissat Bernard , Matheny P. Brandon , Labbé Jessy , Martin Francis M. 
Published: February 2021

AbstractThe ectomycorrhizal symbiosis is an essential guild of many forested ecosystems and has a dynamic evolutionary history across kingdom Fungi, having independently evolved from diverse types of saprotrophic ancestors. In this study, we seek to identify genomic features of the transition to the ectomycorrhizal habit within the Russulaceae, one of the most diverse lineages of ectomycorrhizal fungi. We present comparative analyses of the pangenome and gene repertoires of 21 species across the order Russulales, including a closely related saprotrophic member of Russulaceae. The ectomycorrhizal Russulaceae is inferred to have originated around the Cretaceous-Paleogene extinction event (73.6-60.1 million years ago (MY)). The genomes of the ectomycorrhizal Russulaceae are characterized by a loss of genes for plant cell-wall degrading enzymes (PCWDEs), an expansion of genome size through increased transposable element (TE) content, a reduction in secondary metabolism clusters, and an association of genes coding for certain secreted proteins with TE “nests”. The saprotrophic sister group of the ectomycorrhizal Russulaceae,Gloeopeniophorella convolvens, possesses some of these aspects (e.g., loss of some PCWDE and protease orthologs, TE expansion, reduction in secondary metabolism clusters), resulting from an accelerated rate of gene evolution in the shared ancestor of Russulaceae that predates the evolution of the ectomycorrhizal habit. Genomes of Russulaceae possess a high degree of synteny, including a conserved set of terpene secondary metabolite gene clusters. We hypothesize that the evolution of the ectomycorrhizal habit requires premodification of the genome for plant root association followed by an accelerated rate of gene evolution within the secretome for host-defense circumvention and symbiosis establishment.
Generation and validation of a CRF1:Cre transgenic rat: The role of central amygdala CRF1in nociception and anxiety-like behavior
Authors: Weera Marcus M. , Agoglia Abigail E. , Douglass Eliza , Jiang Zhiying , Rajamanickam Shivakumar , Shackett Rosetta S. , Herman Melissa A. , Justice Nicholas J. , Gilpin Nicholas W. 
Published: February 2021

AbstractCorticotropin-releasing factor type-1 (CRF1) receptors are critical to stress responses because they allow neurons to respond to CRF released in response to stress. Our understanding of the precise role of CRF1-expressing neuronal populations in CRF-mediated behaviors has been largely limited to mouse experiments due to the lack of genetic tools available to selectively visualize and manipulate CRF1+cells in rats. Here, we describe the generation and validation of a transgenic CRF1:Cre-tdTomato rat, which expresses a bicistroniciCre-2A-tdTomatotransgene directed by 200kb of promoter and enhacer sequence surrounding theCrhr1cDNA present within a BAC clone, that has been transgenically inserted into the rat genome. We report thatCrhr1andCremRNA expression are highly colocalized in CRF1:Cre-tdTomato rats within both the central amygdala (CeA), composed of mostly GABAergic neurons, and in the basolateral amygdala (BLA), composed of mostly glutamatergic neurons. In the CeA, membrane properties, inhibitory synaptic transmission, and responses to CRF bath application intdTomato+neurons are similar to those previously reported in GFP+cells in CRFR1-GFP mice. We show that stimulatory DREADD receptors can be selectively targeted to CeA CRF1+cells via virally delivered Cre-dependent transgenes, that transfected Cre/tdTomato+cells are activated by clozapine-n-oxidein vitroandin vivo, and that activation of these cellsin vivoincreases anxiety-like behavior and nocifensive responses. Given the accuracy of expression in the CRF1:Cre rat, modern genetic techniques used to investigate the anatomy, physiology, and behavioral function of CRF1+neurons and circuits can now be performed in assays that require the use of rats as the model organism.Impact StatementA novel transgenic rat for studying the role of specific corticotropin-releasing factor type-1 receptor-expressing cell populations in physiology and behavior.
MetaPro: A scalable and reproducible data processing and analysis pipeline for metatranscriptomic investigation of microbial communities
Authors: Taj Billy , Adeolu Mobolaji , Xiong Xuejian , Ang Jordan , Nursimulu Nirvana , Parkinson John 
Published: February 2021

AbstractBackgroundWhole microbiome RNASeq (metatranscriptomics) has emerged as a powerful technology to functionally interrogate microbial communities. A key challenge is how best to process, analyze and interpret these complex datasets. In a typical application, a single metatranscriptomic dataset may comprise from tens to hundreds of millions of sequence reads. These reads must first be processed and filtered for low quality and potential contaminants, before being annotated with taxonomic and functional labels and subsequently collated to generate global bacterial gene expression profiles.ResultsHere we present MetaPro, a flexible, massively scalable metatranscriptomic data analysis pipeline that is cross-platform compatible through its implementation within a Docker framework. MetaPro starts with raw sequence read input (single end or paired end reads) and processes them through a tiered series of filtering, assembly and annotation steps. In addition to yielding a final list of bacterial genes and their relative expression, MetaPro delivers a taxonomic breakdown based on the consensus of complementary prediction algorithms, together with a focused breakdown of enzymes, readily visualized through the Cytoscape network visualization tool. We benchmark the performance of MetaPro against two current state of the art pipelines and demonstrate improved performance and functionality.ConclusionMetaPro represents an effective integrated solution for the processing and analysis of metatranscriptomic datasets. Its modular architecture allows new algorithms to be deployed as they are developed, ensuring its longevity. To aid user uptake of the pipeline, MetaPro, together with an established tutorial that has been developed for educational purposes is made freely available athttps://github.com/ParkinsonLab/MetaPro. The software is freely available under the GNU general public license v3.
Structure guided engineering of a cold active esterase expands substrate range though a stabilisation mutation that allows access to a buried water chamber
Authors: Noby Nehad , Johnson Rachel L. , Tyzack Jonathan D. , Embaby Amira M. , Saeed Hesham , Hussein Ahmed , Khattab Sherine N. , Rizkallah Pierre J. , Jones D. Dafydd 
Published: February 2021

AbstractCold active esterases represent an important class of enzymes capable of undertaking useful chemical transformations at low temperatures. EstN7 fromBacillus cohniirepresents a true psychrophilic esterase with a temperature optimum below 20°C. We have recently determined the structure of EstN7 and have used this knowledge to understand substrate specificity and expands its substrate range through protein engineering. Substrate range is determined by a plug at the end of acyl binding pocket that blocks access to a buried water filled cavity, so limiting EstN7 to turnover of C2 and C4 substrates. Data mining revealed a potentially important commercial reaction, conversion of triacetin to only the 1,2-glyceryl diacetate isomer, which the EstN7 could achieve. Residues M187, N211 and W206 were identified as plug residues. M187 was identified as the key plug residue but mutation to alanine destabilised the structure as whole. Another plug mutation, N211A had a stabilising effect on EstN7 and suppressed the destabilising M187A mutation. The M187A-N211A variant had the broadest substrate range, capable of hydrolysing a C8 substrate. Thus, the structure of EstN7 together with focused engineering has provided new insights into the structural stability and substrate specificity that allowed expansion of substrate range.
Inhalable Nanobody (PiN-21) prevents and treats SARS-CoV-2 infections in Syrian hamsters at ultra-low doses
Authors: Nambulli Sham , Xiang Yufei , Tilston-Lunel Natasha L. , Rennick Linda J. , Sang Zhe , Klimstra William B. , Reed Douglas S. , Crossland Nicholas A. , Shi Yi , Duprex W. Paul 
Published: February 2021

AbstractGlobally there is an urgency to develop effective, low-cost therapeutic interventions for coronavirus disease 2019 (COVID-19). We previously generated the stable and ultrapotent homotrimeric Pittsburgh inhalable Nanobody 21 (PiN-21). Using Syrian hamsters that model moderate to severe COVID-19 disease, we demonstrate the high efficacy of PiN-21 to prevent and treat SARS-CoV-2 infection. Intranasal delivery of PiN-21 at 0.6 mg/kg protects infected animals from weight loss and substantially reduces viral burdens in both lower and upper airways compared to control. Aerosol delivery of PiN-21 facilitates deposition throughout the respiratory tract and dose minimization to 0.2 mg/kg. Inhalation treatment quickly reverses animals’ weight loss post-infection and decreases lung viral titers by 6 logs leading to drastically mitigated lung pathology and prevents viral pneumonia. Combined with the marked stability and low production cost, this novel therapy may provide a convenient and cost-effective option to mitigate the ongoing pandemic.
Glia-neuron signaling mediated by two different BMP ligands impacts synaptic growth
Authors: Bartoletti Mathieu , Knight Tracy , Held Aaron , Rand Laura M. , Wharton Kristi A. 
Published: February 2021

ABSTRACTThe nervous system is a complex network of cells whose interactions provide circuitry necessary for an organism to perceive and move through its environment. Revealing the molecular basis of how neurons and non-neuronal glia communicate is essential for understanding neural development, behavior, and abnormalities of the nervous system. BMP signaling in motor neurons, activated in part by retrograde signals from muscle expressed Gbb (BMP5/6/7) has been implicated in synaptic growth, function and plasticity inDrosophila melanogaster. Through loss-of-function studies, we establish Gbb as a critical mediator of glia to neuron signaling important for proper synaptic growth. Furthermore, the BMP2/4 ortholog, Dpp, expressed in a subset of motor neurons, acts by autocrine signaling to also facilitate neuromuscular junction (NMJ) growth at specific muscle innervation sites. In addition to signaling from glia to motor neurons, autocrine Gbb induces signaling in larval VNC glia which strongly express the BMP type II receptor, Wit. In addition to Dpp’s autocrine motor neuron signaling, Dpp also engages in paracrine signaling to adjacent glia but not to neighboring motor neurons. In one type of dorsal midline motor neuron, RP2,dpptranscription is under tight regulation, as its expression is under autoregulatory control in RP2 but not aCC neurons. Taken together our findings indicate that bi-directional BMP signaling, mediated by two different ligands, facilitates communication between glia and neurons. Gbb, prominently expressed in glia, and Dpp acting from a discrete set of neurons induce active Smad-dependent BMP signaling to influence bouton number during neuromuscular junction growth.
Apoptotic Extracellular Vesicles (ApoEVs) Safeguard Liver Homeostasis and RegenerationviaAssembling an ApoEV-Golgi Organelle
Authors: Sui Bingdong , Wang Runci , Chen Chider , Kou Xiaoxing , Wu Di , Tu Ho-Chou , Wang Yanzhuang , Liu Yijing , Jacobson Orit , Chen Xiaoyuan , Liu Haixiang , Kwok Ryan Tsz Kin , Tang Ben Zhong , Yan Hexin , Wang Minjun , Xiang Lei , Yan Xutong , Fu Yu , Zhang Xiao , Tang Jianxia , Ma Lan , Lu Lu , Jin Yan , Shi Songtao 
Published: February 2021

SummaryApoptosis is an integral physiological cell death process that occurs frequently and generates a huge number of apoptotic extracellular vesicles (apoEVs). However, whether apoEVs are necessary for maintaining organ homeostasis remains unclear. Here, we show that circulatory apoEVs engraft in liver and undergo specialized internalization by hepatocytes (HCs) based on surface signature of galactose andN-acetylgalactosamine. Furthermore, apoEVs rescue liver injury in apoptotic-deficientFasmutant andCaspase-3knockout mice, which is exerted by restoring the featured hepatic ploidy homeostasis. Surprisingly, apoEVs form a chimeric organelle complex with recipient Golgi apparatusviaSNARE-mediated membrane interaction, which consequently facilitates microtubule organization and HC cytokinesis. Notably, through Golgi recovery and ploidy transition, apoEVs contribute to liver regeneration and protect against acute hepatic failure. Collectively, these results identify a previously unrecognized role for apoEVs and the specific mechanisms by which they safeguard liver homeostasis, and suggest the potential of apoEV-based therapy for liver disorders.
Simultaneous production decisions in agricultural contexts: an experimental investigation of pesticide use, animal welfare and wheat production
Authors: Höhler Julia , Müller Jörg 
Published: February 2021

PurposeFarmers often decide simultaneously on crop production or input use without knowing other farmers' decisions. Anticipating the behavior of other farmers can increase financial performance. This paper investigates the role of other famers' behaviors and other contextual factors in farmers' simultaneous production decisions.Design/methodology/approachMarket entry games are a common method for investigating simultaneous production decisions. However, so far they have been conducted with abstract tasks and by untrained subjects. The authors extend market entry games by using three real contexts: pesticide use, animal welfare and wheat production, in an incentivized framed field experiment with 323 German farmers.FindingsThe authors find that farmers take different decisions under identical incentive structures for the three contexts. While context plays a major role in their decisions, their expectations about the behavior of other farmers have little influence on their decision.Originality/valueThe paper offers new insights into the decision-making behavior of farmers. A better understanding of how farmers anticipate the behavior of other farmers in their production decisions can improve both the performance of individual farms and the allocational efficiency of agricultural and food markets.
Using life goals to explore subjective well-being of people in northeast Thailand
Authors: Promphakping Buapun , Somaboot Pornpen , Mee-Udon Farung , Rattanaprathum Nopparat , Weeranakin Pattaraporn , Promphakping Ninlawadee , Suparatanagool Sungkom 
Published: February 2021

PurposeThe purpose of this paper is to explore changes in life goal importance and subjective well-being, and to uncover if life goal importance predicts subjective well-being of people in the northeast of Thailand.Design/methodology/approachThe data of two surveys conducted in 2006 (n = 184) and 2016 (n = 184) were used. The samples were selected by using multiple sampling techniques and subsampled by age and gender. The data were analysed using descriptive statistics, independent t-test and multiple linear regression.FindingsPaired sample t-test revealed that the change of individual parameters under extrinsic life goals are most evident, these parameters include owning a big house, free of debts, owning amenities, living in clean and pleasant environment, having fewer children, children obtaining formal education, good behaviour of children and good health. The authors also found that two parameters under relational life goal were found different (good family relationship, hosting community events and activities). Satisfaction with living conditions deteriorated while satisfaction with life improved. The model from 2005 confirms that life goal importance predicts subjective well-being, while the opposite with the 2016 model. The direction of prediction of extrinsic, intrinsic and relational life goal importance on subjective well-being between 2006 and 2016 models are in opposite direction.Originality/valueThe study extends the authors understanding of the link between the importance of life goals and subjective well-being, which was previously confined to the advancement towards life goals (goal achievement).
Work–family enrichment: an integrative review
Authors: Agrawal Monika , Mahajan Ritika 
Published: February 2021

PurposeThe purpose of this study is to provide an integrative review of work–family enrichment literature.Design/methodology/approachThe study is based upon 206 peer-reviewed papers derived from systematic search in the Scopus database. The review ascertains its relevance by analyzing the publication trends, research designs, data analysis techniques, sample characteristics, measures and findings of selected articles.FindingsThe study notes a rising trend of publication activity in the Asian region on this topic. Empirical research using regression is the most popular research design in this field. Most of the research studies have targeted samples based on family type and/or gender. The majority of the studies include directionality to measure work–family enrichment. Notably, work-related antecedents and consequences have received the most attention from the research community. Crossover models have concentrated on spousal dyad samples.Originality/valueAs per the authors' knowledge, the study is a primary attempt to integrate the extant literature on this subject. Additionally, the study presents the gender role ideology as a potential moderator and provides future research directions along with managerial implications.
Leveraging green skills in response to the COVID-19 crisis: a case study of small and medium enterprises in Harare, Zimbabwe
Authors: Manyati Tarisai Kudakwashe , Mutsau Morgen 
Published: February 2021

Purpose
The sustainable skills that informal manufacturers use in volatile times remain poorly understood. This study explored the skills that the informal manufacturers used to navigate the uncertain business environment during the COVID-19 pandemic.

Design/methodology/approach
A total of 27 telephone interviews were conducted with informal entrepreneurs who were manufacturers of agricultural machinery at Mbare Magaba and Gaza home industry in Harare, Zimbabwe. Purposive and snow ball sampling were used to identify information rich sources. The authors used thematic analysis in identifying recurrent themes from this study.

Findings
The study results show that business agility motivated most informal manufacturers to restructure their business to sustain their operations. With the closure of formal companies, the informal manufacturers adopted slowing and narrowing loops through purchasing broken down agricultural machines for repairs or remanufacturing of durable machines for their low-income customer base. Most young and formally trained manufacturers adopted a prospector strategic behaviour as they used digital platforms to network with business associates for supplies, gathering market intelligence, making and receiving electronic payments and establishing virtual distant markets whilst the older manufacturers resorted mostly to a defender strategic behaviour of engaging their usual customers for repair jobs and a few referrals.

Originality/value
This study offers unique insights with respect to skills required for the sustainable and strategic management of small and medium enterprises during times of crisis.
Bank stockholders’ vs bank bondholders’ market discipline during crisis time: an investigation based on supervisory stress test information disclosure
Authors: Daouda Dala Moustapha 
Published: February 2021

Purpose
This paper aims to investigate how stockholders and bondholders react to the information disclosed on the financial markets during crisis periods. This paper considers the 2011 European Banking Authority’s stress test as it disclosed detailed information about banks.

Design/methodology/approach
It was conducted during the European sovereign debt crisis, and this paper uses an event study methodology. This paper analyzes the average cumulative abnormal returns for different subsamples of banks. This paper compares the reactions of stockholders and bondholders to the stress test by considering pre-results announcements (signal generating process) to the publication of the results on the disclosure date, using quantitative data for each individual bank that participated in the stress test (the signal provided to the financial market).

Findings
This paper finds that stockholders’ reaction is more sensitive to idiosyncratic components of the disclosed information, whereas bondholders are more influenced by systematic risk. A deeper investigation shows that subordinated bondholders tend to behave quite similarly to stockholders. This specific reaction of stockholders during financial distress may make them more likely than bondholders to impose market discipline during troubled periods.

Originality/value
This paper brings several new insights to the behavior of stock and bond holders during times of financial distress and makes recommendations to regulators that may serve to refine communication to markets to reduce the shock of negative news.
Welfare conditionality, sanctions and homelessness: meanings made by homeless support workers
Authors: Veasey Kelly , Parker Jonathan 
Published: February 2021

Purpose
This study aims to explore homeless-support workers’ perceptions of homeless welfare recipients and their experiences of navigating new conditions placed upon them by UK welfare reform. It examines support workers’ views of the most punitive feature of the welfare system, sanctions, on those recipients.In 2012, the Conservative and Liberal Democrat Coalition Government introduced the largest and most radical overhaul of the UK benefit system, significantly increasing the level of conditionality and sanctions for non-compliance, part of a shift in welfare, suggesting that rights must be balanced by responsibility and the “culture of worklessness” and “benefit dependency” should be addressed.

Design/methodology/approach
Welfare reforms in the UK and the increased use of sanctions as part of welfare conditionality are reviewed. Data are collected from eight semi-structured interviews taking place in five housing support groups in the South East and South West of England in 2019–2020. The interviews followed an approach from interpretive phenomenological analysis.

Findings
Findings from this study indicate that the government’s reforms serve as a disciplinary measure for the poor, reinforcing inequality and social marginalization. To mitigate the effects would require a comprehensive review of universal credit prior to its full rollout to claimants. Data are analyzed thematically.

Originality/value
Welfare conditionality and welfare reform is well-researched in the UK. There is also a significant volume of research concerning homelessness. This paper, however, fills a gap in research concerning the experiences of those working in housing support agencies working with homeless people in the UK.