bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cell-Tak coating may cause mis-normalization of Seahorse metabolic flux data
Authors: Sima Michal , Martinkova Stanislava , Kafkova Anezka , Pala Jan , Trnka Jan 
Published: March 2021

AbstractMetabolic flux investigations of cells and tissue samples are a rapidly advancing tool in diverse research areas. Reliable methods of data normalization are crucial for an adequate interpretation of results and to avoid a misinterpretation of experiments and incorrect conclusions. The most common methods for metabolic flux data normalization are to cell number, DNA and protein. Data normalization may be affected by a variety of factors, such as density, healthy state, adherence efficiency, or proportional seeding of cells.The mussel-derived adhesive Cell-Tak is often used to immobilize poorly adherent cells. Here we demonstrate that this coating may strongly affect the fluorescent detection of DNA leading to an incorrect and highly variable normalization of metabolic flux data. Protein assays are much less affected and cell counting can virtually completely remove the effect of the coating. Cell-Tak coating also affects cell shape in a cell line-specific manner and may change cellular metabolism.Based on these observations we recommend cell counting as a gold standard normalization method for Seahorse metabolic flux measurements with protein content as a reasonable alternative.
Drosophilaantimicrobial peptides and lysozymes regulate gut microbiota composition and abundance
Authors: Marra A. , Hanson M.A. , Kondo S. , Erkosar B. , Lemaitre B. 
Published: March 2021

AbstractThe gut microbiota affects the physiology and metabolism of animals and its alteration can lead to diseases such as gut dysplasia or metabolic disorders. Several reports have shown that the immune system plays an important role in shaping both bacterial community composition and abundance inDrosophila, and that immune deficit, especially during aging, negatively affects microbiota richness and diversity. However, there has been little study at the effector level to demonstrate how immune pathways regulate the microbiota. A key set ofDrosophilaimmune effectors are the antimicrobial peptides (AMPs), which confer defense upon systemic infection. AMPs and lysozymes, a group of digestive enzymes with antimicrobial properties, are expressed in the gut and are good candidates for microbiota regulation. Here, we take advantage of the model organismDrosophila melanogasterto investigate the role of AMPs and lysozymes in regulation of gut microbiota structure and diversity.Using flies lacking AMPs and newly generated lysozyme mutants, we colonized gnotobiotic flies with a defined set of commensal bacteria and analyzed changes in microbiota composition and abundance in vertical transmission and aging contexts through 16S rRNA gene amplicon sequencing. Our study shows that AMPs and, to a lesser extent, lysozymes are necessary to regulate the total and relative abundance of bacteria in the gut microbiota. We also decouple the direct function of AMPs from the IMD signaling pathway that regulates AMPs but also many other processes, more narrowly defining the role of these effectors in the microbial dysbiosis observed in IMD-deficient flies upon aging.ImportanceThis study advances current knowledge in the field of host-microbe interactions by demonstrating that the two families of immune effectors, antimicrobial peptides and lysozymes, actively regulate the gut microbiota composition and abundance. Consequences of the loss of these antimicrobial peptides and lysozymes are exacerbated during aging, and their loss contributes to increased microbiota abundance and shifted composition in old flies. This work shows that immune effectors, typically associated with resistance to pathogenic infections, also help shape the beneficial gut community, consistent with the idea that host-symbiont interactions use the same ‘language’ typically associated with pathogenesis.
Free vibration and buckling analysis of FGM plates using inverse trigonometric shear deformation theory
Authors: Sah Supen Kumar , Ghosh Anup 
Published: March 2021

Purpose
The purpose of this paper is to carry out free vibration and buckling analysis of functionally graded material (FGM) plate.

Design/methodology/approach
Equilibrium and stability equations of FGM rectangular plate under different boundary conditions are derived using finite element method-based inverse trigonometric shear deformation theory (ITSDT). Eight-noded rectangular plate element with seven degrees of freedom at each node is used for the present analysis. The power-law distribution method has been considered for the continuously graded variation in composition of the ceramic and metal phases across the thickness of a functionally graded plate.

Findings
The finite element formulation incorporated with ITSDT and provisions of the constitutive model of FGM plate has been implemented in a numerical code to obtain the natural frequency and critical buckling load under uniaxial and biaxial compressive load. The influence of material gradation, volume fraction index, span to thickness ratio and boundary constraints over free vibration and buckling response has been studied.

Originality/value
Development and validation of finite element methodology using ITSDT to predict the structural response of the FGM plates under different loading, geometric and boundary conditions.
Rotating flow thorough parallel plates with the various inclined magnetic field under the influence of hall current
Authors: Pichaimuthu Chandrasekar , Swaminathan Ganesh 
Published: March 2021

Purpose
The Purpose of this study to examine the magneto hydrodynamics (MHD) using the analytical and numerical tool. In recent years, MHD growing tremendously due to the presence of multidisciplinary application in solving the tedious problems in the viscous flow.

Design/methodology/approach
The flows between the parallel plates under the steady inclined magneto hydrodynamic force were studied under the presence of different hall current and pressure gradient. The system was designed with the Darcian porous medium subjected to the incompressible flow. To analyse the flow reactions through stationary parallel plates, the governing equations were used using the integral transformation.

Findings
The velocity of the flows depends on the Hall parameter. As the intensity of the magnetic field increases the velocity of the flow is affected significantly. On the other hand, the radiation parameters also affect the flow of any medium through the porous medium.

Practical implications
Implementation of the Laplace and Fourier transform increases the reliability of the obtained results and further decreases the uncertainty during the measurement of the velocity of the flow without any restraints.

Originality/value
From the evident results, it is clear that the proposed MHD model can be applied to several operations of the fluid dynamic models. Further, the application of this technique will decrease the uncertainty in the results compared to the conventional computational models and other finite element and difference approaches.
The role of ecological modernization principles in advancing circular economy practices: lessons from the brewery sector
Authors: Sehnem Simone , Lopes de Sousa Jabbour Ana Beatriz , Conceição Diogo Amarildo da , Weber Darciana , Julkovski Dulcimar José 
Published: March 2021

PurposeThere is no literature that sought the revision and integration of ecological modernization of theory (EMT) and circular economy (CE). This article aims to answer the questions: How have companies developed circular economy practices within their operations? Why have companies invested in circular economy practices? Based on the answers to these questions, it would be possible to understand how the assumptions of EMT explain the adoption of CE practices.Design/methodology/approachThe research method is based on multiple case studies with Brazilian craft brewery companies.FindingsCompanies studied have pursed the adoption of circular economy practices, but the full potential of the use of biological cycles within their processes of production has been hampered by the lack of external policies and clear governance toward circular economy. Thus, pollution prevention policies may be an important driver for organizations be able to advance in circular economy practices.Research limitations/implicationsResearch limitations are coverage only of organizations located in an emerging country. A second limitation is that the data saturation was partially achieved in some of the analyzed cases, especially by the limitation of the time period surveyed (transversal research). The longitudinal evaluation is timely for circular economy surveys.Practical implicationsThe formulation of a regulatory framework through a participative, interactive and decentralized process, capable of delegating responsibilities and incentives to all entities, to contemplate a tax restructuring for the alcoholic beverage sector, could corroborate to create a new standard of action for the sector.Social implicationsThis document highlights how the principles of ecological modernization can contribute to the advancement of the circular economy. The understanding can help in the design of processes that provide circular and sustainable operations as a social good in a effective value.Originality/valueThe principles from EMT, such as the development of an institutional environment towards corporate environmental proactive, could enhance the adoption of circular economy practices within the brewery sector. Despite of the class association of the brewery sector has supported the companies studied to move forward in other matters, a clear governance is necessary to guide organizations towards circular economy practices.
Multidimensional behavioral responses to the implementation of BIM in construction projects: an empirical study in China
Authors: Cao Dongping , Shao Shiting , Huang Bin , Wang Guangbin 
Published: March 2021

PurposeDespite its great potential to improve the sustainability of architectural, engineering, construction and facility management activities, the implementation of building information modeling (BIM) in many projects has failed to achieve expected benefits due to negative behavioral responses such as user resistance. This paper aims to characterize the complexity of end user's behavioral responses to BIM implementation in construction projects using a multidimensional perspective and examines how these responses are impacted by different levels of contextual factors.Design/methodology/approachBy integrating technology acceptance, resistance and adoption literature, this paper theoretically proposes a research model to characterize the associations between different dimensions of behavioral responses and different levels of contextual factors. The model is then empirically tested with survey and interview data collected from BIM-based construction projects in China.FindingsThe empirical results not only validate the two-dimensional view of the behavioral responses (i.e. the dimension of support/resistance that ranges from aggressive resistance to enthusiastic support, and the dimension of actual use that ranges from non-use to high use) but also provide evidence for the prevalence of ambivalent responses such as supporting but lowly using and resisting but highly using. The empirical results also provide evidence that different levels of contextual factors generally play different roles in shaping the behavioral responses. Specifically, the dimension of support/resistance is more substantially impacted by the team-level factor while the dimension of actual use of BIM is more significantly associated with the project-level factor.Originality/valueWhile previous research on BIM adoption or implementation behaviors has primarily focused on investigating users' response from single-dimension perspectives such as acceptance or non-acceptance, this study represents an exploratory effort of using a two-dimensional view to characterize the complexity and ambivalence of end users' behavioral responses to the implementation of innovative technologies such as BIM in construction projects. This study also contributes to deepened understandings of how these different dimensions of behavioral responses are intricately shaped by different levels (i.e. individual-, team- and project-levels) of contextual factors in construction projects which are characterized as temporary and inter-organizational.
Investigating digital storytelling for the creation of positively engaging digital content
Authors: Nicoli Nicholas , Henriksen Kine , Komodromos Marcos , Tsagalas Dimitrios 
Published: March 2021

PurposeThis study explores how digital storytelling (DST) approaches can be used for social media campaigns to create more engaging digital content. The ability to better engage with networked publics offers benefits to entities of different scale and scope, since in doing so they establish stronger relationships with their consumers and publics.Design/methodology/approachA digital discourse analysis combined with a five-layer coded film analysis is applied to a DST video, viewed on Facebook.FindingsFour overarching and overlapping approaches are identified. These are emotional appeal based on clear human ideals, equality and simplicity of characters, simplicity and universal representations.Research limitations/implicationsSimilar studies are required across varying targeted digital stories of different length and subject matter to distinguish effectiveness.Practical implicationsDespite advanced technological capacity for audience segmentation, social media campaigns often include unengaging content. DST offers universal characteristics that can be used by entities to engage with their consumers and publics.Social implicationsDST has been used to create learning and pedagogical environments and more participative democracies. Yet its use to strategically engage with networked publics is empirically lacking. The findings of the study can facilitate more effective digital content strategies for entities of all purposes to pursue.Originality/valueFew studies have sought to deconstruct effective short form DST for strategic purposes. This study applies a methodological approach best suited for analysing digital content. The findings provide insights into how strategists and social media managers can create more engaging digital content.
How school-based health education can help young people navigate an uncertain world
Authors: Dixon Rachael , Robertson Jenny 
Published: March 2021

PurposeThe COVID-19 pandemic has provided us a striking demonstration that the future is dynamic, unpredictable, complex and volatile. It is increasingly important that those working in the field of school-based health education reimagine the possibilities and potential of the subject to rise to the challenges presented and make a difference in learners' worlds. In this paper we explore the potential of health education learning to contribute to aspects of the Organization for Economic Co-operation and Development's (OECD's) Learning Compass 2030 from our perspective in Aotearoa New Zealand. This is a learning framework that uses the metaphor of navigation to demonstrate the competencies young people need in order to thrive in the world and has a significant focus on wellbeing for people and society (OECD, 2019).Design/methodology/approachWe explore the links between the learning compass and a socio-critical approach to secondary school-based health education learning opportunities by producing and refining our own knowledge of the learning contexts and experiences that could potentially contribute to the elements of compass. We present this as dialogue produced through asynchronous online conversations between the paper's two authors across a three-month period in 2020 – a method befitting our COVID-19 times.FindingsAfter employing a deductive thematic analysis we found extensive links between health education learning and aspects of the compass which are congruent with the notion that it is more about how the subject is taught than what is covered in a socio-critical health education. We communicate our findings by organising them into three themes that arose for us in analysis: learners' capability to understand the world, navigate the world and change the world.Originality/valueWe conclude the paper with key questions to consider if we are to reimagine school-based health education in order for learning experiences in the subject to enrich learners' understanding of how to navigate the complex and uncertain times they will face across their lives.
Analysis of the mental health response to COVID-19 and human rights concerns in the occupied Palestinian territories
Authors: Helbich Maria , Jabr Samah 
Published: March 2021

Purpose
The coronavirus (COVID-19) pandemic has devastating effects around the world, influencing daily life and putting communities into unprecedented situations of anxiety, hardship and loss. It has a particularly severe effect on the mental health of individuals and highlights pre-existing challenges in mental health provision in different countries. The purpose of this paper is to examine the mental health response to COVID-19 in the occupied Palestinian territories (oPt) in relation to mental health concerns and the political situation.

Design/methodology/approach
This study analyzes the double struggle of Palestinians not only dealing with COVID-19 but with the ongoing Israeli occupation and human rights violations and focuses on the challenges in providing mental health services due to existing inequalities, systemic discrimination and lack of resources as a result of the political system of oppression. The findings are based on previously published articles concerning mental health related to the COVID-19 outbreak in other countries, as well as the authors’ clinical experience in the oPt and direct involvement in providing mental health services.

Findings
The paper highlights how the current pandemic is being used to further attempts of annexation and political gains in Israel and how it exacerbated human rights violations due to the occupation. Emphasis is also put on the challenges in providing a Palestinian mental health response due to the high number of actors involved and the lack of preparedness at the level of mental health response provision.

Originality/value
The value of the works lies in putting the current pandemic in relation to human rights violations in the oPt due to the ongoing Israeli occupation and in highlighting how a mental health response to COVID-19 can be implemented during a state of emergency and despite a lack of preparedness in response services in the oPt.
Automatic detection of optic disc using distance regularized level-set segmentation for glaucoma screening system
Authors: Patil Naganagouda , Patil Preethi N. , Rao P.V. 
Published: March 2021

PurposeThe abnormalities of glaucoma have high impact on deciding and representing the causes that effects severity of blindness in human beings. The simulation experimental results would help the ophthalmologist in diagnosing of glaucoma abnormality accurately. The significant effect of glaucoma has a huge impact on the quality of human life, and its growth rate in world population tremendously increases. Glaucoma is considered as second largest cause for the blindness in the world; hence identification of it marks the importance of its detection at the earliest.Design/methodology/approachThe prime objective of the work proposed is to build up a human intervention free image preparing framework for glaucoma screening. The disc calculation is assessed on retinal image dataset called retinal Image for glaucoma Analysis. The proposed method briefs a novel optic disc division calculation depending on applying a level-set strategy on a confined optic disc image. In the instance of low quality image, a twofold level set is designed, in which the principal level set is viewed as restriction for the optic disc. To keep the veins from meddling with the level-set procedure, an inpainting strategy has been applied. Also a significant commitment is to include the varieties in notion adopted by the ophthalmologists in distinguishing the disc localization and diagnosing the glaucoma. Most of the past investigations are prepared and tested depending on just a single feature, which can be thought to be one-sided for the ophthalmologist.FindingsIn continuation, the correctness has been determined depending on the quantity of image that matched with the investigation pattern adopted by the ophthalmologist. The 175 retinal images were utilized to test the results of proposed work with the manual markings of ophthalmologists. The error-free calculation in marking the optic disc region and centroid was 98.95% in comparison with the existing result of 87.34%.Originality/valueIn continuation, the correctness has been determined depending on the quantity of image that matched with the investigation pattern adopted by the ophthalmologist. The 175 retinal images were utilized to test the results of proposed work with the manual markings of ophthalmologists. The error-free calculation in marking the optic disc region and centroid was 98.95% in comparison with the existing result of 87.34%.
Efficient recruitment with effective job advertisement: an exploratory literature review and research agenda
Authors: Mahjoub Ali , Kruyen Peter Mathieu 
Published: March 2021

PurposeThis article presents an exploratory, narrative review on job ads research. It aims to explore the key features of job ads that have been investigated in previous researches; the way these features have been investigated; and to draw important lessons that those studies teach us about the impact of job ad features on the target population.Design/methodology/approachIn this study, the scoping-review procedure is applied. Its systematic procedure enables scholars to provide a broad overview of a topic, map the key concepts underpinning a research area, clarify the conceptual boundaries of a topic, and also to incorporate a numerical summary and qualitative thematic analysis. The review was conducted based on a systematic study of 243 peer-reviewed articles and publications in the grey literature.FindingsThe findings show that seven job ad features seem to have important impacts on (potential) applicants, which we illustrate as a know-how framework. Eight main theories are used, and a wide array of research methods are applied. However, the study concludes that after more than four decades of research, there is still a limited understanding about the concrete effects of job ad features.Originality/valueThis paper synthesizes the existing knowledge, answers three exploratory questions regarding job ad features and draws theoretical and practical lessons from previous studies. In the interest of conducting future studies and providing a research agenda, a typology of theoretical perspectives for the study of job ads is also presented. The article also presents lessons for practitioners by providing a know-how framework on the usage of job ads.