bluuebunny/crossref_metadata_2025_split huggingface dataset:
Behavioral and brain mechanisms mediating conditioned flight behavior in rats
Authors: Totty Michael S. , Warren Naomi , Huddleston Isabella , Ramanathan Karthik R. , Ressler Reed L. , Oleksiak Cecily R. , Maren Stephen 
Published: April 2021

AbstractEnvironmental contexts can inform animals of potential threats, though it is currently unknown how context biases the selection of defensive behavior. Here we investigated context-dependent flight responses with a Pavlovian serial-compound stimulus (SCS) paradigm that evokes freeze-to-flight transitions. Similar to previous work in mice, we show that male and female rats display context-dependent flight-like behavior in the SCS paradigm. Flight behavior was dependent on contextual fear insofar as it was only evoked in a shock-associated context and was reduced in the conditioning context after context extinction. Flight behavior was only expressed to white noise regardless of temporal order within the compound. Nonetheless, rats that received unpaired SCS trials did not show flight-like behavior to the SCS, indicating it is associative. Finally, we show that pharmacological inactivation of two brain regions critical to the expression of contextual fear, the central nucleus of the amygdala (CeA) and bed nucleus of the stria terminalis (BNST), attenuates both contextual fear and flight responses. All of these effects were similar in male and female rats. This work demonstrates that contextual fear can summate with cued and innate fear to drive a high fear state and transition from post-encounter to circa-strike defensive modes.
Performance and quality attributes of okra (Abelmoschus esculentus (L.) Moench) fruits grown under soil applied Zn-fertilizer, green biomass and poultry manure
Authors: Aboyeji Christopher Muyiwa , Dahunsi Samuel Olatunde , Olaniyan Deborah Oluwatosin , Dunsin Oluwagbenga , Adekiya Aruna Olasekan , Olayanju Adeniyi 
Published: April 2021

AbstractField experiments were carried out in 2018 and 2019 cropping seasons at Landmark University Teaching and Research farm, Omu-Aran Kwara state, Nigeria, to determine the effect of soil applied Zn-fertilizer, Tithonia diversifolia (Ti), Chromolaena odorata (Ch) and poultry manure (PM) on the performance, yield, minerals and vitamins composition of okra fruits. Treatments were combined and tested as follows:—Control (T1), Control + 10 kg ha−1 Zn (T2), 5 t ha−1 Ti + 5 t ha−1 PM (T3), 5 t ha−1 Ch + 5 t ha−1 PM (T4), 5 t ha−1 Ch + 5 t ha−1 Ti (T5), 10 t ha−1 Ti + 0 kg ha−1 Zn (T6), 10 t ha−1 Ti + 10 kg ha−1 Zn (T7), 10 t ha−1 Ch + 0 kg ha−1 Zn (T8), 10 t ha−1 Ch + 10 kg ha−1 Zn (T9), 10 t ha−1 PM + 0 kg ha−1 Zn (T10), and 10 t ha−1 PM + 10 kg ha−1 Zn (T11). The experiment was laid out in a randomized complete block design with four repetitions. Vegetative, yield and quality parameters of okra were taken. Data were subjected to analysis of variance and means were compared using Duncan Multiple Range Test (DMRT) at p ≤ 0.05. Variations were observed on the vegetative parameters, yield, minerals and vitamin composition of okra among the applied amendments. The combined application of green biomass, poultry manure, and Zn-fertilizer improved all the variables tested as compared to when they were applied singly. Application of Zn-fertilizer to some selected plots significantly increased yield, Zn, Mg and vitamins concentration of okra. Application of 5 t ha−1 Ti + 5 t ha−1 PM + 10 kg ha−1 Zn (T3) and 5 t ha−1 Ch + 5 t ha−1 PM + 10 kg ha−1 Zn (T4) significantly improved all the parameters tested but the use of 5 t ha−1 Ti + 5 t ha−1 PM + 10 kg ha−1 Zn (T3) resulted in optimum yield and at the same time increase minerals and vitamin concentration of okra. The results of this study therefore showed that Tithonia diversifolia (Ti) as green biomass contained and released more and quality nutrients than Chromolaena odorata (Ch) when combined with equal rate of Zn fertilizer and poultry manure.
Macrophages with reduced expressions of classical M1 and M2 surface markers in human bronchoalveolar lavage fluid exhibit pro-inflammatory gene signatures
Authors: Takiguchi Hiroto , Yang Chen X. , Yang Cheng Wei Tony , Sahin Basak , Whalen Beth A. , Milne Stephen , Akata Kentaro , Yamasaki Kei , Yang Julia Shun Wei , Cheung Chung Yan , Vander Werff Ryan , McNagny Kelly M. , Leitao Filho Fernando Sergio , Shaipanich Tawimas , van Eeden Stephan F. , Obeidat Ma’en , Leung Janice M. , Sin Don D. 
Published: April 2021

AbstractThe classical M1/M2 polarity of macrophages may not be applicable to inflammatory lung diseases including chronic obstructive pulmonary disease (COPD) due to the complex microenvironment in lungs and the plasticity of macrophages. We examined macrophage sub-phenotypes in bronchoalveolar lavage (BAL) fluid in 25 participants with CD40 (a M1 marker) and CD163 (a M2 marker). Of these, we performed RNA-sequencing on each subtype in 10 patients using the Illumina NextSeq 500. Approximately 25% of the macrophages did not harbor classical M1 or M2 surface markers (double negative, DN), and these cells were significantly enriched in COPD patients compared with non-COPD patients (46.7% vs. 14.5%, p < 0.001). 1886 genes were differentially expressed in the DN subtype compared with all other subtypes at a 10% false discovery rate. The 602 up-regulated genes included 15 mitochondrial genes and were enriched in 86 gene ontology (GO) biological processes including inflammatory responses. Modules associated with cellular functions including oxidative phosphorylation were significantly down-regulated in the DN subtype. Macrophages in the human BAL fluid, which were negative for both M1/M2 surface markers, harbored a gene signature that was pro-inflammatory and suggested dysfunction in cellular homeostasis. These macrophages may contribute to the pathogenesis and manifestations of inflammatory lung diseases such as COPD.
HIV-1 subtype diversity and transmission strain source among men who have sex with men in Guangxi, China
Authors: Chen Yi , Shen Zhiyong , Feng Yi , Ruan Yuhua , Li Jianjun , Tang Shuai , Tang Kailing , Liang Shujia , Pang Xianwu , McNeil Edward B. , Xing Hui , Chongsuvivatwong Virasakdi , Lin Mei , Lan Guanghua 
Published: April 2021

AbstractWith the rapid increase in HIV prevalence of men who have sex with men (MSM) in recent years and common human migration and travelling across different provinces in China, MSM are now finding it easier to meet each other, which might contribute to local HIV epidemics as well as fueling cross-province transmission. We performed a cross-sectional survey in 2018–2019 to investigate the current HIV subtype diversity and inferred HIV strain transmission origin among MSM in Guangxi province, China based on a phylogenetic analysis. Based on 238 samples, we found that the HIV-1 subtype diversity was more complicated than before, except for three major HIV subtypes/circulating recombinant forms (CRFs): CRF07_BC, CRF01_AE, CRF55_01B, five other subtypes/CRFs (CRF59_01B, B, CRF08_BC, CRF67_01B, CRF68_01B) and five unique recombinant forms (URFs) were detected. In total, 76.8% (169/220) of samples were infected with HIV from local circulating strains, while others originated from other provinces, predominantly Guangdong and Shanghai. The high diversity of HIV recombinants and complicated HIV transmission sources in Guangxi MSM indicates that there has been an active sexual network between HIV positive MSM both within and outside Guangxi without any effective prevention. Inter-province collaboration must be enforced to provide tailored HIV prevention and control services to MSM in China.
Effects of transcranial static magnetic stimulation over the primary motor cortex on local and network spontaneous electroencephalogram oscillations
Authors: Shibata Sumiya , Watanabe Tatsunori , Yukawa Yoshihiro , Minakuchi Masatoshi , Shimomura Ryota , Ichimura Sachimori , Kirimoto Hikari , Mima Tatsuya 
Published: April 2021

AbstractTranscranial static magnetic stimulation (tSMS) is a novel non-invasive brain stimulation technique that reduces cortical excitability at the stimulation site. We investigated the effects of tSMS over the left primary motor cortex (M1) for 20 min on the local electroencephalogram (EEG) power spectrum and interregional EEG coupling. Twelve right-handed healthy subjects participated in this crossover, double-blind, sham-controlled study. Resting-state EEG data were recorded for 3 min before the intervention and 17 min after the beginning of the intervention. The power spectrum at the left central electrode (C3) and the weighted phase lag index (wPLI) between C3 and the other electrodes was calculated for theta (4–8 Hz), alpha (8–12 Hz), and beta (12–30 Hz) frequencies. The tSMS significantly increased theta power at C3 and the functional coupling in the theta band between C3 and the parietal midline electrodes. The tSMS over the left M1 for 20 min exhibited modulatory effects on local cortical activity and interregional functional coupling in the theta band. The neural oscillations in the theta band may have an important role in the neurophysiological effects induced by tSMS over the frontal cortex.
A novel tissue specific alternative splicing variant mitigates phenotypes in Ets2 frame-shift mutant models
Authors: Kishimoto Yuki , Nishiura Iori , Hirata Wataru , Yuri Shunsuke , Yamamoto Nami , Ikawa Masahito , Isotani Ayako 
Published: April 2021

AbstractE26 avian leukemia oncogene 2, 3′ domain (Ets2) has been implicated in various biological processes. An Ets2 mutant model (Ets2db1/db1), which lacks the DNA-binding domain, was previously reported to exhibit embryonic lethality caused by a trophoblast abnormality. This phenotype could be rescued by tetraploid complementation, resulting in pups with wavy hair and curly whiskers. Here, we generated new Ets2 mutant models with a frame-shift mutation in exon 8 using the CRISPR/Cas9 method. Homozygous mutants could not be obtained by natural mating as embryonic development stopped before E8.5, as previously reported. When we rescued them by tetraploid complementation, these mice did not exhibit wavy hair or curly whisker phenotypes. Our newly generated mice exhibited exon 8 skipping, which led to in-frame mutant mRNA expression in the skin and thymus but not in E7.5 Ets2em1/em1 embryos. This exon 8-skipped Ets2 mRNA was translated into protein, suggesting that this Ets2 mutant protein complemented the Ets2 function in the skin. Our data implies that novel splicing variants incidentally generated after genome editing may complicate the phenotypic analysis but may also give insight into the new mechanisms related to biological gene functions.
Extra virgin olive oil improved body weight and insulin sensitivity in high fat diet-induced obese LDLr−/−.Leiden mice without attenuation of steatohepatitis
Authors: Álvarez-Amor Leticia , Sierra Amparo Luque , Cárdenas Antonio , López-Bermudo Lucía , López-Beas Javier , Andújar Eloísa , Pérez-Alegre Mónica , Gallego-Durán Rocío , Varela Lourdes M. , Martin-Montalvo Alejandro , Berná Genoveva , Rojas Anabel , Robles-Frías Mª José , Hmadcha Abdelkrim , Romero-Gómez Manuel , Kleemann Robert , Martín Franz 
Published: April 2021

AbstractDietary fatty acids play a role in the pathogenesis of obesity-associated non-alcoholic fatty liver disease (NAFLD), which is associated with insulin resistance (IR). Fatty acid composition is critical for IR and subsequent NAFLD development. Extra-virgin olive oil (EVOO) is the main source of monounsaturated fatty acids (MUFA) in Mediterranean diets. This study examined whether EVOO-containing high fat diets may prevent diet-induced NAFLD using Ldlr−/−. Leiden mice. In female Ldlr−/−.Leiden mice, the effects of the following high fat diets (HFDs) were examined: a lard-based HFD (HFD-L); an EVOO-based HFD (HFD-EVOO); a phenolic compounds-rich EVOO HFD (HFD-OL). We studied changes in body weight (BW), lipid profile, transaminases, glucose homeostasis, liver pathology and transcriptome. Both EVOO diets reduced body weight (BW) and improved insulin sensitivity. The EVOOs did not improve transaminase values and increased LDL-cholesterol and liver collagen content. EVOOs and HFD-L groups had comparable liver steatosis. The profibrotic effects were substantiated by an up-regulation of gene transcripts related to glutathione metabolism, chemokine signaling and NF-kappa-B activation and down-regulation of genes relevant for fatty acid metabolism. Collectivelly, EVOO intake improved weight gain and insulin sensitivity but not liver inflammation and fibrosis, which was supported by changes in hepatic genes expression.
New insights on microscopic properties of metal-porphyrin complexes attached to quartz crystal sensor
Authors: Alyousef Haifa , Alotaibi Badriah M. , Ben Yahia Mohamed , Alanazi Meznah M. , Alsaif Norah A. 
Published: April 2021

AbstractA quartz crystal adsorbent coated with 5,10,15,20-tetrakis(4-methylphenyl) porphyrin was used to examine the complexation phenomenon of three metallic ions [aluminum(III), iron(III) and indium(III)]. The aim is to select the appropriate adsorbate for metalloporphyrin fabrication. The equilibrium adsorption isotherms of tetrakis(4-methylphenyl) porphyrin were performed at four temperatures (from 300 to 330 K) through the quartz crystal microbalance (QCM) method. Subsequently, the experimental data were analyzed in order to develop a thorough explanation of the complexation mechanisms. The experimental results indicated that the aluminum(III) chloride is the adequate material for metalloporphyrin application. Theoretical investigation was established through physics adsorption models in order to analyze the experimental isotherms. The AlCl3 isotherms were modeled via a single-layer adsorption model which is developed using the ideal gas law. Whereas, the FeCl3 isotherms were interpreted via a single-layer adsorption which includes the lateral interactions parameters (real gas law), indicating the lowest stability of the formed iron-porphyrin complex. The participation of the chloride ions in the double-layers adsorption of InCl3 was interpreted via layer by layer formulation. Interestingly, the physicochemical investigation of the three adopted models indicated that the tetrakis(4-methylphenyl) porphyrin adsorption was an endothermic process and that the aluminum(III) chloride can be recommended for an industrial application because it presents the highest adsorption energy (chemical bonds with porphyrins).
Ceramide kinase regulates TNF-α-induced immune responses in human monocytic cells
Authors: Al-Rashed Fatema , Ahmad Zunair , Snider Ashley J. , Thomas Reeby , Kochumon Shihab , Melhem Motasem , Sindhu Sardar , Obeid Lina M. , Al-Mulla Fahd , Hannun Yusuf A. , Ahmad Rasheed 
Published: April 2021

AbstractCeramide kinase (CERK) phosphorylates ceramide to produce ceramide-1-phosphate (C1P), which is involved in the development of metabolic inflammation. TNF-α modulates inflammatory responses in monocytes associated with various inflammatory disorders; however, the underlying mechanisms remain not fully understood. Here, we investigated the role of CERK in TNF-α-induced inflammatory responses in monocytes. Our results show that disruption of CERK activity in monocytes, either by chemical inhibitor NVP-231 or by small interfering RNA (siRNA), results in the defective expression of inflammatory markers including CD11c, CD11b and HLA-DR in response to TNF-α. Our data show that TNF-α upregulates ceramide phosphorylation. Inhibition of CERK in monocytes significantly reduced the secretion of IL-1β and MCP-1. Similar results were observed in CERK-downregulated cells. TNF-α-induced phosphorylation of JNK, p38 and NF-κB was reduced by inhibition of CERK. Additionally, NF-κB/AP-1 activity was suppressed by the inhibition of CERK. Clinically, obese individuals had higher levels of CERK expression in PBMCs compared to lean individuals, which correlated with their TNF-α levels. Taken together, these results suggest that CERK plays a key role in regulating inflammatory responses in human monocytes during TNF-α stimulation. CERK may be a relevant target for developing novel therapies for chronic inflammatory diseases.
Diversification of terpenoid emissions proposes a geographic structure based on climate and pathogen composition in Japanese cedar
Authors: Hiura Tsutom , Yoshioka Hayate , Matsunaga Sou N. , Saito Takuya , Kohyama Tetsuo I. , Kusumoto Norihisa , Uchiyama Kentaro , Suyama Yoshihisa , Tsumura Yoshihiko 
Published: April 2021

AbstractBiogenic volatile organic compounds emitted from plants are important constituents of atmospheric chemistry and play a major role in the resistance of plants against various environmental stresses. However, little is known about how abiotic and biotic environments on a geographic scale relate to diversifications of the emission. Here, we present variations of terpenes stored in and emitted from leaves of a single species in a common garden, using genetically differentiated local populations of Japanese cedar, the most dominant and widely distributed tree species in Japan. Furthermore, we determined the composition of fungal communities in 50 locations, based on the presence or absence of 158 fungal species inhabiting the cedar. The results showed that terpenoids, especially those that are emitted, were highly diversified and geographically structured among the 12 populations. The total amount of stored terpenes was negatively affected by warm and less-snow climates. On the other hand, variations in some emitted terpenoid species among the populations were correlated to antagonistic fungal species inhabiting the Japanese cedar. We propose that the diversification of composition and amount of stored and emitted terpenoids in the tree species is not only structured by climate, but also antagonistic fungal communities through biological interactions.
Impact of physiologically shaped pancreatic stent for chronic pancreatitis
Authors: Hori Yasuki , Ichino Yuka , Naitoh Itaru , Hayashi Kazuki , Yoshida Michihiro , Natsume Makoto , Jinno Naruomi , Kato Akihisa , Kachi Kenta , Asano Go , Atsuta Naoki , Sahashi Hidenori , Kataoka Hiromi , Ohara Hirotaka 
Published: April 2021

AbstractEndoscopic pancreatic stenting is used to prevent main pancreatic duct obstruction and relieve painful symptoms of chronic pancreatitis. However, the stent typically needs to be exchanged and the rate of adverse events is high. Few studies have evaluated the effect of stent shape on those outcomes. We evaluated the adverse events, stent patency, and total medical cost within 90 days of patients who received an 8.5 French (Fr) physiologically shaped pancreatic stent by comparing these features with those associated with a conventional straight-type stent for ≥ 90 days. The total stent-related adverse event rate was significantly lower for the physiologically shaped pancreatic stent (physiologically shaped, 6.7% [2/30]; straight-type, 50.6% [44/87]; P < 0.001). Stent occlusion was significantly less frequent (P < 0.001) and the total medical costs were significantly lower (P = 0.002) for the physiologically shaped stent. The stent-related adverse event rate was significantly higher for the 10 Fr straight type stent than for the 8.5 Fr physiologically shaped stent (10 Fr, straight-type vs. 8.5 Fr, physiologically shaped: 36.1% [13/36] vs. 6.7% [2/30]; P = 0.007). In conclusion, a physiologically shaped pancreatic stent was superior to a straight-type stent in terms of the patency rate and medical costs.
A biological agent modulates the physiology of barley infected with Drechslera teres
Authors: Backes Aurélie , Vaillant-Gaveau Nathalie , Esmaeel Qassim , Ait Barka Essaid , Jacquard Cédric 
Published: April 2021

AbstractRecognized as the causal agent of net blotch,Drechslera teresis responsible for major losses of barley crop yield. The consequences of this leaf disease are due to the impact of the infection on the photosynthetic performance of barley leaves. To limit the symptoms of this ascomycete, the use of beneficial bacteria known as “Plant Growth Promoting Rhizobacteria” constitutes an innovative and environmentally friendly strategy. A bacterium named as strain B25 belonging to the genusBurkholderiashowed a strong antifungal activity againstD. teres. The bacterium was able to limit the development of the fungus by 95% in detached leaves of bacterized plants compared to the non-bacterized control. In this study, in-depth analyses of the photosynthetic performance of young barley leaves infected withD. teresand/or in the presence of the strain B25 were carried out both in and close to the necrotic area. In addition, gas exchange measurements were performed only near the necrotic area. Our results showed that the presence of the beneficial bacterium reduced the negative impact of the fungus on the photosynthetic performance and modified only the net carbon assimilation rate close to the necrotic area. Indeed, the presence of the strain B25 decreased the quantum yield of regulated non-photochemical energy loss in PSII noted as Y(NPQ) and allowed to maintain the values stable of maximum quantum yield of PSII photochemistry known as Fv/Fmand close to those of the control in the presence ofD. teres. To the best of our knowledge, these data constitute the first study focusing on the impact of net blotch fungus and a beneficial bacterium on photosynthesis and respiratory parameters in barley leaves.
Epigenetic alteration contributes to the transcriptional reprogramming in T-cell prolymphocytic leukemia
Authors: Tian Shulan , Zhang Henan , Zhang Pan , Kalmbach Michael , Lee Jeong-Heon , Ordog Tamas , Hampel Paul J. , Call Timothy G. , Witzig Thomas E. , Kay Neil E. , Klee Eric W. , Slager Susan L. , Yan Huihuang , Ding Wei 
Published: April 2021

AbstractT cell prolymphocytic leukemia (T-PLL) is a rare disease with aggressive clinical course. Cytogenetic analysis, whole-exome and whole-genome sequencing have identified primary structural alterations in T-PLL, including inversion, translocation and copy number variation. Recurrent somatic mutations were also identified in genes encoding chromatin regulators and those in the JAK-STAT signaling pathway. Epigenetic alterations are the hallmark of many cancers. However, genome-wide epigenomic profiles have not been reported in T-PLL, limiting the mechanistic study of its carcinogenesis. We hypothesize epigenetic mechanisms also play a key role in T-PLL pathogenesis. To systematically test this hypothesis, we generated genome-wide maps of regulatory regions using H3K4me3 and H3K27ac ChIP-seq, as well as RNA-seq data in both T-PLL patients and healthy individuals. We found that genes down-regulated in T-PLL are mainly associated with defense response, immune system or adaptive immune response, while up-regulated genes are enriched in developmental process, as well as WNT signaling pathway with crucial roles in cell fate decision. In particular, our analysis revealed a global alteration of regulatory landscape in T-PLL, with differential peaks highly enriched for binding motifs of immune related transcription factors, supporting the epigenetic regulation of oncogenes and genes involved in DNA damage response and T-cell activation. Together, our work reveals a causal role of epigenetic dysregulation in T-PLL.