bluuebunny/crossref_metadata_2025_split huggingface dataset:
wingless signal and Zeste-white 3 kinase trigger opposing changes in the intracellular distribution of Armadillo
Authors: Peifer Mark , Sweeton Dari , Casey Michael , Wieschaus Eric 
Published: April 2021

ABSTRACT
wingless/wnt-1 signaling directs cell fate during development. Genetic analysis in Drosophila identified genes that may encode components of the wingless signal transduction system. Drosophila Armadillo, homolog of vertebrate β-catenin, is required for wingless signaling. Unlike armadillo RNA, Armadillo protein accumulates non-uniformly in different cells of each embryonic segment. We found that cells alter their intracellular distribution of Armadillo in response to Wingless signal, accumulating increased levels of cytoplasmic Armadillo relative to those of membrane-associated protein. Levels of cytoplasmic Armadillo are also regulated by Zeste-White 3 kinase. Analysis of double mutants demonstrates that Armadillo’s role in wingless signaling is direct, and that Armadillo functions down-stream of both wingless and zeste-white 3. We present a model for the role of Armadillo stripes in transduction of wingless signal.
The establishment of bilateral asymmetry in sea urchin embryos
Authors: McCain Elizabeth R. , McClay David R. 
Published: April 2021

ABSTRACT
Although much is known about the specification and determination of the two primary axes (animal/vegetal and dorsoventral or oral/aboral) in a number of embryos, little is understood about bilaterality. In the sea urchin, left/right asymmetry is crucial to normal development as the echinus or adult rudiment is positioned on the left side of the larva. We examined the establishment of bilateral asymmetry in Lytechnis variegatus embryos by determining the relationship of the first cleavage planes to the left/right axis. Embryos were bisected at different times to determine when the bilateral axis is committed. These lineage tracing and cell separation experiments demonstrated that the first cleavage plane divides the embryo into left and right halves, although this is conditional until after late blastula stage. The relationship between the specification of the dorsoventral axis and the bilateral axis was examined experimentally. In other species when the dorsal and ventral halves of early echinoderm embryos (preblastula) are separated, the dorsal half often reverses (180°) its dorsoventral axis. We asked whether those larvae with an inverted dorsoventral axis would shift the position of the echinus rudiment from the original left side to the new left side. If so, it would demonstrate that the larval asymmetry is dependent upon specification of the dorsoventral axis. Using a combination of lineage tracing and cell separation techniques, we show that the left/right asymmetry is specified with respect to the dorsoventral axis.
Early transcription inCaenorhabditis elegansembryos
Authors: Edgar Lois G. , Wolf Nurit , Wood William B. 
Published: April 2021

ABSTRACTWe have analysed early transcription in devitellinized, cultured embryos of the nematode Caenorhabditis elegans by two methods: measurement of [32P]UTP uptake into TCA-precipitable material and autoradiographic detection of [3H]UTP labelling both in the presence and absence of α-amanitin. RNA synthesis was first detected at the 8-to 12-cell stage, and α-amanitin sensitivity also appeared at this time, during the cleavages establishing the major founder cell lineages. The requirements for maternally supplied versus embryonically produced gene products in early embryogenesis were examined in the same culture system by observing the effects of α-amanitin on cell division and the early stereotyped lineage patterns. In the presence of high levels of α-amanitin added at varying times from two cells onward, cell division continued until approximately the 100-cell stage and then stopped during a single round of cell division. The characteristic unequal early cleavages, orientation of cleavage planes and lineage-specific timing of early divisions were unaffected by α-amanitin in embryos up to 87 cells. These results indicate that embryonic transcription starts well before gastrulation in C. elegans embryos, but that although embryonic transcripts may have important early functions, maternal products can support at least the mechanics of the first 6 to 7 cell cycles.
Pax-3 expression in segmental mesoderm marks early stages in myogenic cell specification
Authors: Williams Brian A. , Ordahl Charles P. 
Published: April 2021

ABSTRACT
Specification of the myogenic lineage begins prior to gastrulation and culminates in the emergence of determined myogenic precursor cells from the somites. The myoD family (MDF) of transcriptional activators controls late step(s) in myogenic specification that are closely followed by terminal muscle differentiation. Genes expressed in myogenic specification at stages earlier than MDFs are unknown. The Pax-3 gene is expressed in all the cells of the caudal segmental plate, the early mesoderm compartment that contains the precursors of skeletal muscle. As somites form from the segmental plate and mature, Pax-3 expression is progressively modulated. Beginning at the time of segmentation, Pax-3 becomes repressed in the ventral half of the somite, leaving Pax-3 expression only in the dermomyotome. Subsequently, differential modulation of Pax-3 expression levels delineates the medial and lateral halves of the dermomyotome, which contain precursors of axial (back) muscle and limb muscle, respectively. Pax-3 expression is then repressed as dermomyotome-derived cells activate MDFs. Quail-chick chimera and ablation experiments confirmed that the migratory precursors of limb muscle continue to express Pax-3 during migration. Since limb muscle precursors do not activate MDFs until 2 days after they leave the somite, Pax-3 represents the first molecular marker for this migratory cell population. A null mutation of the mouse Pax-3 gene, Splotch, produces major disruptions in early limb muscle development (Franz, T., Kothary, R., Surani, M. A. H., Halata, Z. and Grim, M. (1993) Anat. Embryol. 187, 153-160; Goulding, M., Lumsden, A. and Paquette, A. (1994)Development 120, 957-971). We conclude, therefore, that Pax-3 gene expression in the paraxial mesoderm marks earlier stages in myogenic specification than MDFs and plays a crucial role in the specification and/or migration of limb myogenic precursors.
Expression of inhibin subunits and follistatin during postimplantation mouse development: decidual expression of activin and expression of follistatin in primitive streak, somites and hindbrain
Authors: Albano R. M. , Arkell R. , Beddington R. S. P. , Smith J. C. 
Published: April 2021

ABSTRACT
Members of the activin family are believed to act as mesoderm-inducing factors during early amphibian development. Little is known, however, about mesoderm formation in the mammalian embryo, and as one approach to investigating this we have studied activin and follistatin expression during early mouse development. Activins are homo- or heterodimers of the βA or βB subunits of inhibin, itself a heterodimer consisting of one of the β subunits together with an α subunit. Follistatin is a single-chain polypeptide which inhibits activin function.
Expression of the inhibin α chain could not be detected in embryonic or extraembryonic tissues at any of the stages studied (5.5 to 8.5 days) and expression of the βA and βB subunits could only be observed in the deciduum in cells surrounding the embryo. Expression of follistatin could also be detected in the deciduum, but in a pattern complementary to that of the β subunits. Embryonic expression of follistatin first occurred in the primitive streak, and at later stages transcripts were detectable in the somites and in rhombomeres 2, 4 and 6 of the hindbrain. These results are consistent with a role for activin in mesoderm formation in the mouse embryo, and suggest functions for follistatin in addition to its role as an inhibitor of activin.
Antagonistic activities of Suppressor of Hairless and Hairless control alternative cell fates in the Drosophila adult epidermis
Authors: Schweisguth François , Posakony James W. 
Published: April 2021

ABSTRACT
Successive alternative cell fate choices in the imaginal disc epithelium lead to the differentiation of a relatively invariant pattern of multicellular adult sensory organs in Drosophila. We show here that the activity of Suppressor of Hairless is required for both the sensory organ precursor (SOP) versus epidermal cell fate decision, and for the trichogen (shaft) versus tormogen (socket) cell fate choice. Complete loss of Suppressor of Hairless function causes most proneural cluster cells to accumulate high levels of the achaete and Delta proteins and to adopt the SOP fate. Late or partial reduction in Suppressor of Hairless activity leads to the apparent transformation of the tormogen (socket) cell into a second trichogen (shaft) cell, producing a ‘double shaft’ phenotype. We find that overexpression of Suppres-sor of Hairless has the opposite phenotypic effects. SOP determination is prevented by an early excess of Suppres-sor of Hairless activity, while at a later stage, the trichogen (shaft) cell is transformed into a second tormogen (socket) cell, resulting in ‘double socket’ bristles. We conclude that, for two different cell fate decisions in adult sensory organ development, decreasing or increasing the level of Sup-pressor of Hairless function confers mutant phenotypes that closely resemble those associated with gain and loss of Hairless activity, respectively. These results, along with the intermediate SOP phenotype observed in Suppressor of Hairless; Hairless double mutant imaginal discs, suggest that the two genes act antagonistically to commit imaginal disc cells stably to alternative fates.
The ventral nervous system defective gene controls proneural gene expression at two distinct steps during neuroblast formation in Drosophila
Authors: Skeath James B. , Panganiban Grace F. , Carroll Sean B. 
Published: April 2021

ABSTRACT
Within the Drosophila embryo, the formation of many neuroblasts depends on the functions of the proneural genes of the achaete-scute complex (AS-C): achaete (ac), scute (sc) and lethal of scute (l’sc), and the gene ventral nervous system defective (vnd). Here, we show that vnd controls neuroblast formation, in part, through its regulation of the proneural genes of the AS-C. vnd is absolutely required to activate ac, sc and l’sc gene expression in proneural clusters in specific domains along the medial column of the earliest arising neuroblasts. Using ac-lacZ reporter constructs, we determined that vnd controls proneural gene expression at two distinct steps during neuroblast formation through separable regulatory regions. First, vnd is required to activate proneural cluster formation within the medial column of every other neuroblast row through regulatory elements located 3′ to ac; second, through a 5′ regulatory region, vnd functions to increase or maintain proneural gene expression in the cell within the proneural cluster that normally becomes the neuroblast. By following neuroblast segregation in vnd mutant embryos, we show that the neuroectoderm forms normally and that the defects in neuroblast formation are specific to particular proneural clusters.
The survival of NGF-dependent but not BDNF-dependent cranial sensory neurons is promoted by several different neurotrophins early in their development
Authors: Buj-Bello Anna , Pinon Luzia G. P. , Davies Alun M. 
Published: April 2021

ABSTRACT
Recent work has shown that the survival of the nerve growth factor (NGF)-dependent trigeminal ganglion neurons of the mouse embryo is promoted by brain-derived neurotrophic factor (BDNF) and neurotrophin-3 (NT-3) during the early stages of target field innervation (Buchman and Davies, (1993)Development, 118, 989-1001). The present study was undertaken to ascertain if responsiveness to multiple neurotrophins is a universal feature of the early stages of neuronal development or is restricted to only certain kinds of neurons. To address this issue, we took advantage of the accessibility, from an early developmental stage, of several populations of cranial sensory neurons in the chicken embryo that depend for survival on just one or two known neurotrophins during the phase of naturally occurring cell death. During the mid-embryonic period (E10 to E12) when the number of sensory neurons is declining due to naturally occurring neuronal death, the neurons of the jugular ganglion and the dorsomedial part of the trigeminal ganglion (DMTG) were supported by NGF, the neurons of the ventrolateral part of the trigeminal ganglion (VLTG) were supported by BDNF and the nodose ganglion contained a major subset of neurons supported by BDNF and a minor subset supported by NT-3. Earlier in development (E6), the survival of DMTG and jugular neurons was additionally promoted by BDNF and NT-3. In contrast, E6 VLTG neurons did not exhibit a survival response to either NGF or NT-3, and E6 nodose neurons did not exhibit a survival response to NGF. The loss of the early survival response of DMTG and jugular neurons to BDNF and NT-3 was due to a marked shift in the doseresponse to these neurotrophins (by more than four orders of magnitude between E6 and E8). These findings indicate that the responsiveness of certain populations of neurons to neurotrophins becomes restricted during development and raise the possibility that neuron-target interactions involving multiple neurotrophins regulate neuronal survival in vivo.
Gap gene properties of the pair-rule gene runt during Drosophila segmentation
Authors: Tsai Chihcheng , Gergen J. Peter 
Published: April 2021

ABSTRACT
The Drosophila Runt protein is a member of a new family of transcriptional regulators that have important roles in processes extending from pattern formation in insect embryos to leukemogenesis in humans. We used ectopic expression to investigate runt’s function in the pathway of Drosophila segmentation. Transient over-expression of runt under the control of a Drosophila heat-shock promoter caused stripe-specific defects in the expression patterns of the pair-rule genes hairy and even-skippedbut had a more uniform effect on the secondary pair-rule gene fushi tarazu. Surprisingly, the expression of the gap segmentation genes, which are upstream of runt in the segmentation hierarchy was also altered in hs/runt embryos. A subset of these effects were interpreted as due to an antagonistic effect of runt on transcriptional activation by the maternal morphogen bicoid. In support of this, expression of synthetic reporter gene constructs containing oligomerized binding sites for the Bicoid protein was reduced in hs/runt embryos. Finally, genetic experiments demonstrated that regulation of gap gene expression by runt is a normal component of the regulatory program that generates the segmented body pattern of the Drosophila embryo.
Developmental potential of trunk neural crest cells in the mouse
Authors: Serbedzija George N. , Bronner-Fraser Marianne , Fraser Scott E. 
Published: April 2021

ABSTRACT
The availability of naturally occurring and engineered mutations in mice which affect the neural crest makes the mouse embryo an important experimental system for studying neural crest cell differentiation. Here, we determine the normal developmental potential of neural crest cells by performing in situ cell lineage analysis in the mouse by microinjecting lysinated rhodamine dextran (LRD) into individual dorsal neural tube cells in the trunk. Labeled progeny derived from single cells were found in the neural tube, dorsal root ganglia, sympathoadrenal derivatives, presumptive Schwann cells and/or pigment cells. Most embryos contained labeled cells both in the neural tube and at least one neural crest derivative, and numerous clones contributed to multiple neural crest derivatives. The time of injection influenced the derivatives populated by the labeled cells. Injections at early stages of migration yielded labeled progeny in both dorsal and ventral neural crest derivatives, whereas those performed at later stages had labeled cells only in more dorsal neural crest derivatives, such as dorsal root ganglion and presumptive pigment cells. The results suggest that in the mouse embryo: (1) there is a common precursor for neural crest and neural tube cells; (2) some neural crest cells are multipotent; and (3) the timing of emigration influences the range of possible neural crest derivatives.
The fates of the blastomeres of the 16-cell zebrafish embryo
Authors: Strehlow David , Heinrich Gerhard , Gilbert Walter 
Published: April 2021

ABSTRACT
We present a fate map for the 16-cell-stage blastomeres of the zebrafish embryo Brachydanio rerio. We injected high molecular weight fluorescent dextran into cleavage-stage cells to observe the contributions of the descendants of the first 16 cells to the adult. The patterns derived from these early cells are similar, but not identical among different embryos. Furthermore, two-color injections showed that sister blastomeres at the 16-cell stage regularly contribute to different sets of adult structures. A few of the scored tissues could not be mapped in a manner consistent with the predicted axes. Other tissues were mapped to several of the 16 blastomeres. Many of the tissues map with high probability to a few 16-cell-stage blastomeres. Thus, based on 112 injections in 56 different embryos, we have constructed a fate map by assigning probabilities for the contribution of each blastomere to each of 31 tissues in the 26-hour embryo.
The spatial and temporal dynamics of Sax1(CHox3) homeobox gene expression in the chick’s spinal cord
Authors: Spann Perah , Ginsburg Malka , Rangini Zehava , Fainsod Abraham , Eyal-Giladi Hefzibah , Gruenbaum Yosef 
Published: April 2021

ABSTRACT
Sax1 (previously CHox3) is a chicken homeobox gene belonging to the same homeobox gene family as the Drosophila NK1 and the honeybee HHO genes. Sax1 transcripts are present from stage 2 H&H until at least 5 days of embryonic development. However, specific localization of Sax1 transcripts could not be detected by in situ hybridization prior to stage 8−, when Sax1 transcripts are specifically localized in the neural plate, posterior to the hindbrain. From stages 8− to 15 H&H, Sax1 continues to be expressed only in the spinal part of the neural plate. The anterior border of Sax1 expression was found to be always in the transverse plane separating the youngest somite from the yet unsegmented mesodermal plate and to regress with similar dynamics to that of the segregation of the somites from the mesodermal plate. The posterior border of Sax1 expression coincides with the posterior end of the neural plate. In order to study a possible regulation of Sax1 expression by its neighboring tissues, several embryonic manipulation experiments were performed. These manipulations included: removal of somites, mesodermal plate or notochord and transplantation of a young ectopic notochord in the vicinity of the neural plate or transplantation of neural plate sections into the extraembryonic area. The results of these experiments revealed that the induction of the neural plate by the mesoderm has already occurred in full primitive streak embryos, after which Sax1 is autonomously regulated within the spinal part of the neural plate.
An axial domain of HOM/Hox gene expression is formed by morphogenetic alignment of independently specified cell lineages in the leech Helobdella
Authors: Nardelli-Haefliger Denise , Bruce Ashley E. E. , Shankland Marty 
Published: April 2021

ABSTRACT
The homeobox gene Lox2, a member of the HOM/Hox gene class, is expressed in a restricted domain along the anteroposterior (A-P) body axis of the leech Helobdella. The segmental tissues of the leech embryo arise from the parallel merger of five distinct and bilaterally paired cell lineages generated by embryonic stem cells or teloblasts. Injection of cell lineage tracers coupled with anti-LOX2 immunochemistry reveals that all five teloblast lineages generate central nervous system neurons that express the LOX2 protein, and that each lineage expresses LOX2 within a similar domain of body segments. Some lineally identified neurons display anti-LOX2 immunoreactivity over the entire expression domain, but the OM7 neuron has a distinctively high level of LOX2 expression, which is restricted to the seventh midbody ganglion.
To ascertain the role of positional information in the axial patterning of LOX2 expression, we performed focal cell ablations that displaced one or another of the teloblast lineages out of segmental register with the other axial tissues. Such displacements brought about a corresponding shift in the LOX2 expression of the perturbed lineage, and had little or no effect on the LOX2 expression of the other, unperturbed lineages. This result indicates that the axial domain of LOX2 expression is not specified by positional cues acting coordinately across the various teloblast lineages, nor would it seem that the expression domain is imprinted from one lineage to the others. Rather, the different teloblast lineages acquire their axial patterns independently, and secondarily bring these patterns into alignment along the A-P axis through a process of morphogenetic assembly.
A distal tyrosinase upstream element stimulates gene expression in neural-crest-derived melanocytes of transgenic mice: position-independent and mosaic expression
Authors: Porter Susan D. , Meyer Cathy J. 
Published: April 2021

ABSTRACT
We have assessed the importance of a melanocyte-specific DNase I hypersensitive site and matrix attachment region situated 15 kb upstream of the mouse tyrosinase gene by analysis in transgenic mice. Transgenes containing all, part, or none of this region linked to the tyrosinase promoter and human tyrosinase cDNA were introduced into genetically albino mice, and pigmentation and transgene message levels were analyzed in the resulting transgenic lines. The effect of the upstream region was to enhance significantly gene expression in melanocytes, and to provide position-independent expression of the transgene. Two exceptions to complete position indepen-dence were seen; these lines displayed a mosaic expression pattern in which the transgene was expressed fully in some melanocyte clones but less so in others, resulting in trans-verse stripes of colours ranging from near white to dark grey. Unexpectedly, pigmentation in the eye of all trans-genic lines containing the upstream region was non-uniform, in that the neural-crest-derived melanocytes of the choroid and anterior iris contained significantly more pigment than those derived from the optic cup (retinal pigment epithelium and posterior iris). Transgenes con-taining a small part or none of the upstream region were expressed poorly and in a position-dependent manner; of those lines that were visibly pigmented, expression was equal in the neural crest and optic-cup-derived cells of the eye. Mice with transgenes containing DNA sequences encompassing the hypersensitive site but lacking most of the matrix attachment region were, on average, poorer expressors than those containing the entire upstream region; the highest expressing line of this series, however, had a pigmentation level similar to the lines with the full upstream region. Thus, full transcriptional enhancement activity may lie within the segment containing the hyper-sensitive site, but position-independent expression may require the flanking matrix attachment region containing sequences.