bluuebunny/crossref_metadata_2025_split huggingface dataset:
Proteasomal Degradation of JAZ9 by Salt- and Drought-Induced Ring Finger 1 During Pathogen Infection
Authors: Ramu Vemanna S. , Pal Garima , Oh Sunhee , Mysore Kirankumar S. 
Published: October 2021

E3 ubiquitin ligase salt- and drought-induced ring finger 1 (SDIR1) plays a novel role in modulating plant immunity against pathogens. The molecular interactors of SDIR1 during pathogen infection are not known. SDIR1-interacting jasmonate zinc-finger inflorescence meristem domain (JAZ) proteins were identified through a yeast two-hybrid (Y2H) screen. Full-length JAZ9 interacts with SDIR1 only in the presence of coronatine (a bacteria-secreted toxin) or jasmonic acid (JA) in a Y2H assay. The bimolecular fluorescence complementation and pull-down assays confirm the in planta interaction of these proteins. JAZ9 proteins, negative regulators of JA-mediated plant defense, were degraded during the pathogen infection by SDIR1 through a proteasomal pathway causing disease susceptibility against hemibiotrophic pathogens. [Formula: see text] The author(s) have dedicated the work to the public domain under the Creative Commons CC0 “No Rights Reserved” license by waiving all of his or her rights to the work worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law, 2021.
Transcranial Motor-evoked Potential Alert After Supine-to-Prone Position Change During Thoracic Ossification in Posterior Longitudinal Ligament Surgery
Authors: Yoshida Go , Ushirozako Hiroki , Imagama Shiro , Kobayashi Kazuyoshi , Ando Kei , Ando Muneharu , Kawabata Shigenori , Yamada Kei , Kanchiku Tsukasa , Fujiwara Yasushi , Taniguchi Shinichirou , Iwasaki Hiroshi , Shigematsu Hideki , Takatani Tsunenori , Tadokoro Nobuaki , Takahashi Masahito , Wada Kanichiro , Yamamoto Naoya , Funaba Masahiro , Yasuda Akimasa , Hashimoto Jun , Morito Shinji , Tani Toshikazu , Matsuyama Yukihiro 
Published: October 2021

Study Design.
A prospective, multicenter study.

Objective.
To evaluate the usefulness of transcranial motor-evoked potentials (Tc-MEPs) during supine-to-prone position change for thoracic ossification of the posterior longitudinal ligament (T-OPLL).

Summary of Background Data.
Supine-to-prone position change might be a risk of spinal cord injury in posterior decompression and fusion surgeries for T-OPLL.

Methods.
The subjects were 145 patients with T-OPLL surgically treated with posterior decompression and fusion using Tc-MEPs in 14 institutes. Tc-MEPs were monitored before surgery from supine-to-prone position and intraoperatively in seven institutes and only intraoperatively in the other seven institutes because of disapproval of the anesthesia department. In cases of Tc-MEP alert after position change, we adjusted the cervicothoracic posture. When the MEP did not recover, we reverted the position to supine and monitored the Tc-MEPs in supine position.

Results.
There were 83 and 62 patients with/without Tc-MEP before position change to prone (group A and B). The true-positive rate was lower in group A than group B, but without statistical significance (8.4% vs. 16.1%, P = 0.12). In group A, five patients who had Tc-MEP alert during supine-to-prone position change were all female and had larger body mass index values and upper thoracic lesions. Among the patients, three underwent surgeries after cervicothoracic alignment adjustment, and two had postponed operations to 1 week later with halo-vest fixation because of repeated Tc-MEP alerts during position change to prone. The Tc-MEP alert at exposure was statistically more frequent in group B than in group A (P = 0.033).

Conclusion.
Tc-MEP alert during position change is an important sign of spinal cord injury due to alignment change at the upper thoracic spine. Tc-MEP monitoring before supine-to-prone position change was necessary to prevent spinal cord injury in surgeries for T-OPLL.
Definitive Diagnostic Evaluation of the Child With Arterial Ischemic Stroke and Approaches to Secondary Stroke Prevention
Authors: Lee Sarah , Muthusami Prakash , Wasserman Bruce A. , Heit Jeremy J. , Chandra Ronil V. , Hui Ferdinand , Negrotto Matias , Abruzzo Todd A. 
Published: October 2021

Abstract
In children with arterial ischemic stroke (AIS), the definitive diagnosis of stroke subtype and confirmation of stroke etiology is necessary to mitigate stroke morbidity and prevent recurrent stroke. The common causes of AIS in children are sharply differentiated from the common causes of adult AIS. A comprehensive, structured diagnostic approach will identify the etiology of stroke in most children. Adequate diagnostic evaluation relies on advanced brain imaging and vascular imaging studies. A variety of medical and surgical secondary stroke prevention strategies directed at the underlying cause of stroke are available. This review aims to outline strategies for definitive diagnosis and secondary stroke prevention in children with AIS, emphasizing the critical role of neuroimaging.
The Second Helsinki Face Transplantation and the World Experience of Revision Surgery During the First Posttransplantation Year
Authors: Kiukas Emma-Lotta , Manninen Atte , Lindford Andrew , Lassus Patrik 
Published: October 2021

Introduction
We conducted a review of all surgical interventions performed during the first postoperative year in published face transplantation (FT) cases. In addition, we herein present our second FT patient, a full-face composite FT with an emphasis on surgical revisions after transplantation.

Materials and Methods
A literature review was conducted and resulted in 376 publications of which 33 included reference to surgical interventions during the first post-FT year. For our second FT patient, a thorough review of all medical records was performed.

Results
Among the first 41 FTs, 32 FT patients had reports with reference to corrective surgery during the first year (22 composite and 10 soft-tissue FTs). Soft-tissue FTs had a median of 2 procedures (range, 1–8 procedures), and composite FTs, 3 procedures per patient (range, 1–9 procedures). Nearly all early interventions (<1 month) were performed in composite FT patients (anastomotic occlusion, hematoma, sialocele, palatinal dehiscence). The most common late interventions were scar corrections, tissue suspensions, periorbital corrections, osseal and dental procedures, and interventions for palatinal dehiscence and sialocele. Our second FT patient has recovered well and has undergone 3 surgical interventions during the first year.

Conclusions
Surgical interventions are very common during the first postoperative year after FT, and composite FTs are more prone to complications necessitating surgical intervention than soft-tissue FTs. There is a wide variety of complications reflecting the heterogeneity of FT allografts. Corrective surgery in FT patients appears safe with only a few reported complications.
Direct Neurotization
Authors: Horen Sydney R. , Hamidian Jahromi Alireza , Konofaos Petros 
Published: October 2021

Abstract
Direct neurotization is a method that involves direct implantation of nerve fascicles into a target tissue, that is, muscle fibers, skin, cornea, and so on, with the goal of restoring aesthetic, sensation and or functional capacity. This technique has been implemented since the early 1900s, with numerous experimental and clinical reports of success. Applications have included both sensory and motor neurotization of muscle, as well as protective sensory provision for other organs. These techniques have been used to restore corneal sensation, repair brachial plexus injuries, reestablish tongue movement and function through direct tongue neurotization, and reinnervate multiple facial muscles in patients with facial paralysis. Most recently, these methods have even been used in conjunction with acellular cadaveric nerve grafts to directly neurotize skin. Indications for direct neurotization remain limited, including those in which neural coaptation is not feasible (ie, surgical or traumatic damage to neuromuscular junction, severe avulsion injuries of the distal nerve); however, the success and wide-range application of direct neurotization shows its potential to be implemented as an adjunct treatment in contrast to views that it should solely be used as a salvage therapy. The purpose of the following review is to detail the historic and current applications of direct neurotization and describe the future areas of investigation and development of this technique.
Evaluation of the Effects of Systemic Irisin Hormone Application on Osseointegration of Titanium Implants: An Experimental Study
Authors: Bulmus Ozgur , Dundar Serkan , Bozoglan Alihan , Canpolat Sinan 
Published: October 2021

Abstract
This study aimed to conduct a biomechanical investigation of the effects of systemic irisin hormone application on the osseointegration of titanium implants in rat tibias. After surgical implementation of titanium implants in the metaphyseal part of the tibiae of rats, the rats were randomly divided into 2 equal groups: control group (n = 10) and irisin group (n = 10). After surgery in the control group, the rats received no further treatment during the 4-week experimental period. The rats in the irisin group were given 100 ng/kg irisin every day for the 4-week experimental period after surgery. At the end of the experimental period, the rats were euthanized. implants and surrounding bone tissues were collected for biomechanical (Newton) bone implant connection analysis. The Student t test was used for statistical analysis. There were no significant differences in the biomechanical osseointeration values (Newton) of the groups (P > 0.05, P
= 0.59). Also, in the irisin group, there was numerically but not statistically more bone implant connection than in the controls. Within the limitations of this study, irisin did not affect the osseointegration of titanium implants.
Power dynamics of theta oscillations during goal-directed navigation in freely moving humans: A mobile EEG-virtual reality T-maze study
Authors: Lin Mei-Heng , Liran Omer , Bauer Neeta , Baker Travis E. 
Published: October 2021

AbstractTheta oscillations (∼4–12 Hz) are dynamically modulated by speed and direction in freely moving animals. However, due to the paucity of electrophysiological recordings of freely moving humans, this mechanism remains poorly understood. Here, we combined mobile-EEG with fully immersive virtual-reality to investigate theta dynamics in twenty-two healthy adults (aged 18–29 years old) freely navigating a T-maze to find rewards. Our results revealed three dynamic periods of theta modulation: 1) theta power increases coincided with the participants’ decision-making period; 2) theta power increased for fast and leftward trials as subjects approached the goal location; and 3) feedback onset evoked two phase-locked theta bursts over the right temporal and frontal-midline channels. These results suggest that recording scalp EEG in freely moving humans navigating a simple virtual T-maze can be utilized as a powerful translational model by which to map theta dynamics during “real-life” goal-directed behavior in both health and disease.
Genetic risk factors and Covid-19 severity in Brazil: results from BRACOVID Study
Authors: Pereira Alexandre C. , Bes Taniela Marli , Velho Mariliza , Marques Emanuelle , Jannes Cintia E , Valino Karina Ramos , Dinardo Carla L , Costa Silvia Figueiredo , da Silva Duarte Alberto José , dos Santos Alexandre Ricardo , Mitne-Neto Miguel , Medina-Pestana Jose , Krieger Jose E 
Published: October 2021

AbstractThe Covid-19 pandemic has changed the paradigms for disease surveillance and rapid deployment of scientific-based evidence for understanding disease biology, susceptibility, and treatment. We have organized a large-scale genome-wide association study in Sars-Cov-2 infected individuals in Sao Paulo, Brazil, one of the most affected areas of the pandemic in the country, itself one of the most affected in the world. Here we present the results of the initial analysis in the first 5,233 participants of the BRACOVID study.We have conducted a GWAS for Covid-19 hospitalization enrolling 3533 cases (hospitalized Covid-19 participants) and 1700 controls (non-hospitalized Covid-19 participants). Models were adjusted by age, sex and the 4 first principal components. A meta-analysis was also conducted merging BRACOVID hospitalization data with the Human Genetic Initiative (HGI) Consortia results.BRACOVID results validated most loci previously identified in the HGI meta-analysis. In addition, no significant heterogeneity according to ancestral group within the Brazilian population was observed for the two most important Covid-19 severity associated loci: 3p21.31 and Chr21 near IFNAR2. Using only data provided by BRACOVID a new genome-wide significant locus was identified on Chr1 near the genes DSTYK and RBBP5. The associated haplotype has also been previously associated with a number of blood cell related traits and might play a role in modulating the immune response in Covid-19 cases.
Comparative transmissibility of SARS-CoV-2 variants Delta and Alpha in New England, USA
Authors: Earnest Rebecca , Uddin Rockib , Matluk Nicholas , Renzette Nicholas , Siddle Katherine J. , Loreth Christine , Adams Gordon , Tomkins-Tinch Christopher H. , Petrone Mary E. , Rothman Jessica E. , Breban Mallery I. , Koch Robert Tobias , Billig Kendall , Fauver Joseph R. , Vogels Chantal B.F. , Turbett Sarah , Bilguvar Kaya , De Kumar Bony , Landry Marie L. , Peaper David R. , Kelly Kevin , Omerza Greg , Grieser Heather , Meak Sim , Martha John , Dewey Hannah H. , Kales Susan , Berenzy Daniel , Carpenter-Azevedo Kristin , King Ewa , Huard Richard C. , Smole Sandra C. , Brown Catherine M. , Fink Timelia , Lang Andrew S. , Gallagher Glen R. , Sabeti Pardis C. , Gabriel Stacey , MacInnis Bronwyn L. ,   New England Variant Investigation Team, Tewhey Ryan , Adams Mark D. , Park Daniel J. , Lemieux Jacob E. , Grubaugh Nathan D. 
Published: October 2021

AbstractThe severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Delta variant quickly rose to dominance in mid-2021, displacing other variants, including Alpha. Studies using data from the United Kingdom and India estimated that Delta was 40-80% more transmissible than Alpha, allowing Delta to become the globally dominant variant. However, it was unclear if the ostensible difference in relative transmissibility was due mostly to innate properties of Delta’s infectiousness or differences in the study populations. To investigate, we formed a partnership with SARS-CoV-2 genomic surveillance programs from all six New England US states. By comparing logistic growth rates, we found that Delta emerged 37-163% faster than Alpha in early 2021 (37% Massachusetts, 75% New Hampshire, 95% Maine, 98% Rhode Island, 151% Connecticut, and 163% Vermont). We next computed variant-specific effective reproductive numbers and estimated that Delta was 58-120% more transmissible than Alpha across New England (58% New Hampshire, 68% Massachusetts, 76% Connecticut, 85% Rhode Island, 98% Maine, and 120% Vermont). Finally, using RT-PCR data, we estimated that Delta infections generate on average ∼6 times more viral RNA copies per mL than Alpha infections. Overall, our evidence indicates that Delta’s enhanced transmissibility could be attributed to its innate ability to increase infectiousness, but its epidemiological dynamics may vary depending on the underlying immunity and behavior of distinct populations.
Mice with a humanized immune system are resilient to transplantation of human microbiota
Authors: Zhou Wei , Chow Kin-hoe , Geyer Rory , Peshkepija Paola , Fleming Elizabeth , Yu Chun , Palucka Karolina , Oh Julia 
Published: October 2021

AbstractHuman gut microbiota has co-evolved with human, and plays important roles in regulating the development and functioning of the host immune system. To study the human-specific microbiome-immunune interaction in an animal model is challenging as the animal model needs to capture both the human-specific immune functions and the human-specific microbiome composition. Here we combined two widely-used humanization procedures to generate a humanized mouse model (HMA-huCD34) with functional human leukocytes developed from engrafted huCD34+ cells and human fecal microbes introduced through fecal microbiota transplantation, and investigated how the two introduced human components interact. We found that the engrafted human leukocytes are resilient to the transplanted human microbes, while reciprocally the transplanted microbial community in the huCD34 mice was significantly different from mice without a humanized immune system. By tracking the colonization of human fecal Bacteroides strains in the mouse gut, we found that the composition of the strain population changes over time, the trajectory of which depends upon the type of mouse. On the other hand, different from Bacteroides, Akkermansia muciniphila exhibited consistent and rapid fixation of a single donor strain in all tested mice, suggesting strong purifying selection common to all mouse types. Our prospect study illustrated the complex interactions between the transplanted microbiome and different host factors, and suggested that the humanized mouse model may not faithfully reproduce the human-specific microbiome-immune interaction.
Immediate recruitment of dormant coronary collaterals can provide more than half of normal resting perfusion during coronary occlusion in patients with coronary artery disease
Authors: Reid Brandon J , Lindow Thomas , Warren Stafford , Persson Eva , Bhindi Ravinay , Ringborn Michael , Ugander Martin , Allahwala Usaid 
Published: October 2021

AbstractAimsDormant coronary collaterals are highly prevalent and clinically beneficial in cases of coronary occlusion. However, the magnitude of myocardial perfusion provided by immediate coronary collateral recruitment during acute occlusion is unknown. The aim of this study was to quantify collateral myocardial perfusion during balloon occlusion in patients with coronary artery disease (CAD).Methods and resultsPatients without angiographically visible collaterals undergoing elective percutaneous transluminal coronary angioplasty (PTCA) to a single epicardial vessel underwent two scans with 99mTc-sestamibi myocardial perfusion single-photon emission computed tomography (SPECT). All subjects underwent at least three minutes of angiographically verified complete balloon occlusion, at which time an intravenous injection of the radiotracer was administered, followed by SPECT imaging. A second radiotracer injection followed by SPECT imaging was performed 24 hours after PTCA.The study included 22 patients (median [interquartile range] age 68 [54-72] years, 10 (45%) female). The perfusion defect extent was 19 [11–38] % of the LV, and the collateral perfusion at rest was 64 [58-67]% of normal.ConclusionThis is the first study to describe the magnitude of short-term changes in coronary microvascular collateral perfusion in patients with CAD. On average, despite coronary occlusion and an absence of angiographically visible collateral vessels, collaterals provided more than half of the normal perfusion.Graphical abstractDormant collaterals are highly prevalent but the magnitude of myocardial perfusion provided by immediate coronary collateral recruitment during acute occlusion in humans is unknown. Patients without angiographically visible collaterals underwent intravenous injection with 99mTc-sestamibi during coronary balloon inflation followed by SPECT imaging. A control scan was performed 24 hours later..Median collateral perfusion during coronary occlusion was 64% of normal.
Torque ripples reduction in a synchronous reluctance motor by rotor parameters optimization
Authors: Naeimi Mohammad Reza , Abbaszadeh Karim , Nasiri-Zarandi Reza 
Published: October 2021

Purpose
High torque ripple is the significant challenge of the synchronous reluctance machine in household electric appliances, electric vehicles and so on. This paper aims to present an optimized design of a synchronous reluctance rotor structure to reduce the torque ripple with improving the average torque by the particle swarm optimization (PSO) algorithm.

Design/methodology/approach
The optimization of rotor geometries has been investigated. Most of the rotor parameters such as the width of iron parts, the width of barriers along d and q axes and the endpoint angle of barriers are optimized by a new method using the PSO algorithm. After optimization, the resulted optimum design along with the initial design is simulated by two-dimensional finite element method and results are compared. At the end, a prototype is constructed and tested. Results of the experiment are compared with the simulation results where acceptable adoption is yielded.

Findings
Minimizing the torque ripple without losing the average torque is an important achievement of the synchronous reluctance motor (SynRM) optimization; furthermore, the finite element analysis and experimental results indicate that the torque ripple of the SynRM with the optimized rotor is reduced significantly. Also, increasing the number of optimization parameters can effectively obtain an accurate shape of the SynRM barrier.

Originality/value
Because of the high number of parameters in synchronous reluctance rotors, the majority of proposed optimizations did not use all geometric parameters of the rotor and tried to simplify the optimization by ignoring several optimization parameters or reducing the number of flux barriers. In this optimization, most of the rotor parameters have been used to achieve the precise barrier shape with the aim of reducing the torque ripple in SynRM.
Evacuation of vulnerable people during a Natech: a case study of a flood and factory explosion in Japan
Authors: Ohtsu Nobuhito , Hokugo Akihiko , Cruz Ana Maria , Sato Yukari , Araki Yuko , Park Hyejeong 
Published: October 2021

PurposeThis study investigated pre-evacuation times and evacuation behaviors of vulnerable people during the 2018 flooding in Shimobara, Okayama, Japan, and the flood-triggered factory explosion, a natural hazard-triggered technological accident known as a natural-hazard-triggered technological accidents (Natech). This study examined factors that affected evacuation decisions and pre-evacuation time, estimated the evacuation time in case of no explosion and identified community disaster prevention organization response efforts for vulnerable people.Design/methodology/approachInterviews with all 18 vulnerable people who experienced the event were conducted. Multiple regression analysis was used to examine the effect of six factors on evacuation time and reasons for delayed evacuation.FindingsFactors affecting evacuation decisions included the sound of the explosion, followed by recommendations from relatives and the community disaster prevention organization. Explosion-related injuries delayed early evacuation, but experience of previous disasters and damage had a positive effect on early evacuation. The explosion sound accelerated evacuation of non-injured people; however, explosion-related injuries significantly delayed evacuation of injured individuals. The Shimobara community disaster prevention organization’s disaster response included a vulnerable people registry, visits to all local households and a multilayered approach that enabled monitoring of all households.Originality/valueThis is the first study to examine the evacuation behavior of vulnerable people and community responses during a Natech event.