bluuebunny/crossref_metadata_2025_split huggingface dataset:
Likely pathogenic variants of SDC3, KCNA2, KCNK1, KCNK16 and HSF1 are in support of acquired irreversible PIEZO2 channelopathy in ALS onset
Authors: Sonkodi Balázs , Nagy Zsófia Flóra , Keller-Pintér Anikó , Klivényi Péter , Széll Márta 
Published: January 2025

Abstract

Amyotrophic lateral sclerosis is a multisystem progressive neurodegenerative disease. A recent theory of ALS proposed that the disease initiating primary damage is an acquired irreversible intrafusal proprioceptive terminal PIEZO2 channelopathy with underlying genetic and environmental risk factors. Underpinning this theory that these intrafusal proprioceptive terminal PIEZO2 ion channels initiate an ultrafast proton-based oscillatory signaling to motor neurons through VGLUT1 and to the hippocampus through VGLUT2. This acquired progressive and irreversible intrafusal proprioceptive terminal PIEZO2 channelopathy may gradually degenerate motor neurons in which process Kv1.2 ion channels are gradually depleted. Furthermore, it also gradually depletes heat shock transcription factor-1 in the hippocampus, hence negatively affecting adult hippocampal neurogenesis. In addition, not only PIEZO2-PIEZO2 crosstalk is fully disrupted progressively in ALS between proprioceptive primary afferent terminals and hippocampus due to lost PIEZO2 initiated cross-coupled Huygens synchronization, but PIEZO2-PIEZO1 crosstalk is also disrupted on the periphery. Syndecans, especially syndecan-3 in the nervous system, are critical players in the maintenance of this PIEZO crosstalk. The detected charge altering variants of syndecan-3 likely promotes the impairment of the PIEZO crosstalk, and the progressive loss of the proposed proton-based signaling to motor neurons and to the hippocampus as well. Variants of KCNA2 may also facilitate the faster loss of Kv1.2 ion function on motor neurons when PIEZO channelopathy prevails, not to mention that potassium current rectifying ion channels encoding KCNK1 and KCNK16 variants may also propel ALS disease process and provide the autoimmune-like pathogenic background. Moreover, PIEZO2 channelopathy likely promotes diminishing heat shock transcription factor-1 function in the hippocampus in the presence of the identified HSF1 variants, leading to impaired adult hippocampal neurogenesis.
Multi-omics Approaches to Uncover Liquid-Based Cancer-Predicting Biomarkers in Lynch Syndrome
Authors: Kärkkäinen Minta , Sievänen Tero , Korhonen Tia-Marje , Tuomikoski Joonas , Pylvänäinen Kirsi , Äyrämö Sami , Seppälä Toni T. , Mecklin Jukka-Pekka , Laakkonen Eija K. , Jokela Tiina 
Published: January 2025

Abstract

Background
Lynch syndrome is a genetic cancer-predisposing syndrome caused by pathogenic mutations in DNA mismatch repair (path_MMR) genes. Due to the elevated cancer risk, novel screening methods, alongside current surveillance techniques could enhance cancer risk stratification. Here we show how multi-omics integration could be utilized to pinpoint cancer-predicting biomarkers in Lynch Syndrome. We studied which blood-based circulating microRNAs and metabolites could predict Lynch Syndrome cancer occurrence within a 5.8-year prospective surveillance period.
Methods
The study cohort consisted of 116 Lynch Syndrome carriers who were healthy at the time of sampling, of whom 17 developed cancer during the surveillance. Principal Coordinate Analysis and Canonical Correlation Analysis were used to explore the relationships between single and multi-omics data, enabling the identification of patterns and correlations across different biological layers. Weighted Correlation Network Analysis was used to identify omics-level co-expression modules and to study how these modules are associated with future cancer incidence or path_MMR variant. Lasso Cox regression was used to identify cancer-predicting biomarkers. The initial model was internally validated by splitting the data randomly into 5 training and corresponding validation datasets. Biological functions of future cancer-associated circulating microRNAs were studied by conducting pathway analyses using miRWalk.
Results
Weighted Correlation Network Analysis revealed a circulating microRNA co-expression module significantly associated with future cancer incidence. The identified microRNAs regulate cancer-related pathways including PI3K/Akt signaling pathway. Also, the analysis detected a circulating metabolite module, consisting of ApoB containing lipoprotein classes, (low-, intermediate-, and very low-density lipoproteins), and included cholesterols, as well as phospholipids and sphingomyelins, that had distinct levels between the path_MMRvariants. Three biomarkers- hsa-miR-101-3p, hsa-miR-183-5p, and the among of triglycerides in high-density lipoprotein particles (HDL_TG)- significantly predicted cancer risk based on Lasso Cox regression, with a C-index of 0.76 (p-value = 0.0007), where elevated levels of these biomarkers were indicators of increased hazard ratio. In the internal validation, the model had an average C-index of 0.72.
Conclusions
The multi-omics approach and the identified biomarkers offer a promising tool for cancer risk identification in Lynch Syndrome while also uncovering underlying systemic molecular mechanisms.
Fluence rate-dependent kinetics of light-triggered liposomal doxorubicin assessed by quantitative fluorescence-based endoscopic probe
Authors: Rohrbach Daniel J. , Carter Kevin A. , Luo Dandan , Shao Shuai , Aygun-Sunar Semra , Lovell Jonathan F. , Sunar Ulas 
Published: January 2025

Abstract

Liposomal doxorubicin (Dox), a treatment option for recurrent ovarian cancer, often suffers from suboptimal biodistribution and efficacy, which might be addressed with precision drug delivery systems. Here, we introduce a catheter-based endoscopic probe designed for multispectral, quantitative monitoring of light-triggered drug release. This tool utilizes red-light photosensitive porphyrin-phospholipid (PoP), which is encapsulated in liposome bilayers to enhance targeted drug delivery. By integrating diffuse reflectance and fluorescence spectroscopy, our approach not only corrects the effects of tissue optical properties but also ensures accurate drug delivery to deep-seated tumors. Preliminary results validate the probe effectiveness in controlled settings, highlighting its potential for future clinical adaptation. This study sets the stage for in vivo applications, enabling the exploration of next-generation treatment paradigms for the management of cancer by optimizing chemotherapy administration with precision and control.
Impact of Power Law Coefficient on Buoyant Heat Transfer in Porous Trapezoidal Enclosures
Authors: M VIJAYA KUMARA V , M ASWATHA , Kumar.R Vasanth , C.B Hemanth Kumar , G Mohan Kumar , Thejaraju.R  , Manjunatha.C  
Published: January 2025

Abstract

The impact of the power-law coefficient on buoyant energy transfer in porous trapezoidal cavities is investigated in this study. The analysis focuses on non-Newtonian fluids, where the flow behaviour deviates from the classical Newtonian model and the power-law coefficient plays a important role in defining the fluid’s rheological properties. Partial differential equations are formulated using the stream function approach and solved with an in-house MATLAB code and validated against previously published work .The cavity geometry combined with a porous medium, introduces complex thermal and flow interactions. The study examines how varying the power-law coefficient influences heat transfer rates, velocity profiles and temperature distribution within the cavity. Numerical simulations are conducted under different Rayleigh numbers (Ra = 100 to 2000) and boundary conditions demonstrating that the power-law coefficient significantly affects the thermal performance of the system. Results indicate that higher power-law coefficients enhance convective heat transfer, while lower coefficients lead to more diffusive heat transport. This research provides valuable insights into optimizing energy transfer in industrial applications involving Newtonian and non-Newtonian fluids in porous media.
Robust Estimates of Earth System Predictability of the 1st kind using the CESM2 Multiyear Prediction System (CESM2-MP)
Authors: Kim Yong-Yub , Lee June-Yi , Timmermann Axel , Chikamoto Yoshimitsu , Lee Sun-Seon , Kwon Eun Young , Park Wonsun , Hasan Nahid A. , Bethke Ingo , Fransner Filippa , Karwat Alexia , Subrahmanian Abhinav R. , Franzke Christian L. E. 
Published: January 2025

Abstract

Here we present a new seasonal-to-multiyear Earth prediction system (CESM2-MP) based on the Community Earth System Model version 2 (CESM2). A 20-member ensemble which assimilates oceanic temperature and salinity anomalies provides the initial conditions for 5-year predictions from 1960 to 2020. We analyze skills using pairwise ensemble statistics, calculated among individual ensemble members (IM) and compare the results with the more commonly used ensemble mean (EM) approach. This comparison is motivated by the fact that an EM of a nonlinear dynamical system generates – unlike reality – a heavily smoothed trajectory, akin to a slow manifold evolution. However, for most autonomous nonlinear systems, the EM does not even represent a solution of the underlying physical equations, and it should therefore not be used as an estimate of the expected trajectory. The IM-based approach is less sensitive to the ensemble size than EM-based skill computations, and its estimates of potential predictability are closer to the actual skill. Using IM-based statistics helps to unravel the physics of predictability patterns in CESM-MP and their relationship to ocean-atmosphere-land interactions and climate modes. Furthermore, the IM-based method emphasizes potential predictability of the 1st kind which is associated with the propagation of the initial conditions. In contrast, the EM-based method is more sensitive to predictability of the 2nd kind, which is associated with the external forcing and time-varying boundary conditions. Calculating IM-based skills for the CESM-MP provides new insights into predictability sources due to ocean initial conditions and helps delineate and quantify forecast limits of internal variability.
An Analysis of the Effect of Economic Growth, Education, and Health on Poverty in East Java
Authors: Qudsi Hanif , Ashar Khusnul 
Published: January 2025

This study aims to analyze the impact of economic growth, education, and health on poverty in East Java Province during the 2018-2023 period. This study is motivated by the high poverty rate in East Java compared to other provinces in Indonesia, despite having a smaller population. The Human Capital Theory and Amartya Sen's Poverty Theory are used as theoretical foundations. The data used is secondary data from Badan Pusat Statistik (BPS), covering 38 districts/cities in East Java Province. The analytical method used is multiple linear regression with panel data. The results of the study show that economic growth, education, and health have a significant negative effect on the poverty rate. Economic growth measured through GDP, the average length of schooling as an education indicator, and life expectancy as a health indicator, all contribute to the reduction of poverty. These findings indicate the importance of improvements in the economic, education, and health sectors to reduce poverty in East Java. The implications of this study can help the government and other stakeholders formulate effective policies for poverty alleviation in the region.

Abstrak
Penelitian ini bertujuan untuk menganalisis pengaruh pertumbuhan ekonomi, pendidikan, dan kesehatan terhadap kemiskinan di Provinsi Jawa Timur selama periode 2018-2023. Penelitian ini dilatarbelakangi oleh tingginya tingkat kemiskinan di Jawa Timur dibandingkan provinsi lain di Indonesia, meskipun dengan jumlah penduduk yang lebih sedikit. Teori Human Capital dan Teori Kemiskinan Amartya Sen digunakan sebagai landasan teoretis. Data yang digunakan merupakan data sekunder dari Badan Pusat Statistik (BPS), mencakup 38 kabupaten/kota di Provinsi Jawa Timur. Metode analisis yang digunakan adalah regresi linier berganda dengan data panel. Hasil penelitian menunjukkan bahwa pertumbuhan ekonomi, pendidikan, dan kesehatan berpengaruh negatif signifikan terhadap tingkat kemiskinan. Pertumbuhan ekonomi yang diukur melalui PDRB, rata-rata lama sekolah sebagai indikator pendidikan, dan angka harapan hidup sebagai indikator kesehatan, semuanya berkontribusi terhadap penurunan kemiskinan. Temuan ini mengindikasikan pentingnya peningkatan dalam sektor ekonomi, pendidikan, dan kesehatan untuk mengurangi kemiskinan di Jawa Timur. Implikasi penelitian ini dapat membantu pemerintah dan pemangku kepentingan lainnya dalam merumuskan kebijakan yang efektif untuk pengentasan kemiskinan di daerah tersebut.
Awareness of Passive Smoking Among the Adult Population in Northern Borders and Al Jouf regions, Saudi Arabia
Authors: M Abd El Mawgod Mohamed , Alruwaili Khalid Hamdan , Alruwaili Wael Lafi , Alruwaili Majed Lafi 
Published: January 2025

Objectives: To assess the knowledge of passive smoking (PS) among the adult population and their understanding of its harmful effects on their health.
Methods: A cross-sectional survey was carried out through social media channels (Twitter, and WhatsApp Snapchat).
Results: The study had 414 participants in total, their mean age was 35.7±11.5, the majority (73.9%; 306) were males, and married participants comprise a significant portion of the population (62.3%, 258). Most of the participants reported having family members who smoke. The vast majority of respondents (78.5%, 325) believed that PS experienced the same risks as active smokers.
Conclusion: The majority of adults in Saudi Arabia's Northern and Al Jouf regions were exposed to passive smoking. More than 60% of respondents believed that being close to someone who smokes causes you to start smoking. Most people thought that PS increased children's risk of pneumonia and that passive smokers had the same hazards as active smokers.
THE EFFECT OF STEAM ON STUDENT LEARNING ACTIVITIES IN ELEMENTARY SCHOOL
Authors: Rahayu Nina , Amanahtillah Dara , Wulandari Febriyantika , Jelita Jelita 
Published: January 2025

The low learning activity of grade V students of MIIT Al-Mubarak Langsa related to science is due to the lack of active students in learning so that it is necessary to have an approach that can increase student learning activities STEAM. This study aims to determine the effect of STEAM on student learning activities in science learning grade V MIIT Al-Mubarak Langsa in the 2022/2023 school year. This type of research is quantitative research. All fifth grade students of MIIT Al-Mubarak Langsa totaling 34 students are the population while the sample of this study is students from two classes, namely 17 VA class students as the experimental class and 17 VB class students as the control class. Data collection techniques in this study were in the form of observation sheets, with data analysis using SPSS 24. Hypothesis testing using the Independent Sample Test t test. Based on the hypothesis testing of learning activities in experimental and control classes, the tcount> ttable is 20.873> 2.036 and a significant value of 0.00 <0.05. So it can be concluded that HO is rejected and Ha is accepted at the significance level a=0.05, which means that there is an effect of the STEAM approach on student learning activities.Keywords: Activity, Student Learning, Science, STEAM
ANALYZING STUDENT MOTIVATIONS FOR SELECTING PGMI AS THEIR MAJOR
Authors: Darmiah Darmiah , Marvida Tria 
Published: January 2025

This study examines the motivations behind students’ choices to pursue a major in Primary Islamic Education Teacher Training (PGMI) at the Faculty of Tarbiyah and Teacher Training, UIN Ar-Raniry Banda Aceh. PGMI aims to prepare graduates with competencies in foundational Islamic education and child development, equipping them to be creative, productive, and independent educators. Given the varied internal and external motivations influencing student decisions, understanding these motivations is essential, as they play a crucial role in academic success and career satisfaction. Accurate choice of major can significantly impact student achievement and future opportunities, while an inappropriate choice may hinder career potential. Employing a quantitative, descriptive-exploratory approach, this study seeks to answer the question, "What motivates students to choose PGMI as their major?" Participants included PGMI students from the 2019 cohort at UIN Ar-Raniry Banda Aceh. Data collection involved questionnaires and observations, with results analyzed through descriptive statistics. Findings revealed two primary motivational factors influencing students’ choice of PGMI: (1) a strong aspiration to become teachers and (2) respect for parental expectations. This research contributes insights into student motivation, supporting universities in aligning programs with student interests and career goals. Keywords: Student Motivation, Madrasah Ibtidaiyah Teacher Education, PGMI, Major Selection, Faculty of Tarbiyah and Teacher Training, UIN Ar-Raniry, Career Motivation
Acidente vascular cerebral: percepções dos enfermeiros na classificação de risco
Authors: Da Silva Lara Stefanny , Vieira Isabela , Streit Leandro Janayna Aparecida , De Freitas Milarch Carine 
Published: January 2025

O objetivo do estudo foi identificar as percepções dos enfermeiros em relação à classificação de risco na identificação dos sintomas de AVC. O acidente vascular cerebral, AVC, ocorre quando os vasos responsáveis pelo transporte do sangue até o cérebro se rompem ou entopem, e com isso, acaba provocando a paralisia da região cerebral que ficou sem circulação sanguínea. Ao apresentar os sintomas do AVC em uma Unidade de Pronto Atendimento, o paciente é atendido inicialmente por um enfermeiro, que tem a responsabilidade de identificar os sinais e sintomas e encaminhar o paciente para atendimento imediato. Foi realizada uma pesquisa de campo descritiva com abordagem qualitativa, entrevistando 12 Enfermeiros graduados em Enfermagem, atuantes nas Unidades de Pronto Atendimento, em Joinville. A coleta de dados ocorreu mediante a aplicação de um questionário presencialmente com perguntas abertas sobre o tema e sobre o profissional enfermeiro. Inicialmente houve um período de ambientação na classificação de risco, que foi o cenário do estudo, com os participantes e as pesquisadoras para, posteriormente, propor-se o questionário individual com cada profissional. Os dados foram coletados no período de Agosto a Outubro de 2023 e exclusivamente presencial com cada enfermeiro participante. Foi utilizado um instrumento criado pelas pesquisadoras com perguntas abertas sobre o tema e o processamento dos dados segue a prerrogativa de Minayo, na análise temática. Em vista disso, a pesquisa respeitou os preceitos éticos da resolução 510/2016 do CNS. Participaram da pesquisa doze enfermeiros, com idades entre 25 e 55 anos, e com um tempo de experiência na área de urgência e emergência variando de cinco a quinze anos. Foi possível observar, com base nos resultados obtidos através da pesquisa, que alguns enfermeiros se sentem inseguros em atender pacientes que estejam apresentando sintomas de AVC, principalmente nos casos em que já existem outras comorbidades associadas ou quando os sintomas são apresentados em pacientes idosos. Evidenciou-se com a pesquisa, que os enfermeiros atuantes na classificação de risco prezam por um atendimento otimizado e eficaz quando identificam pacientes com sintomas de AVC na classificação de risco. Alguns enfermeiros não possuem especialização na área de urgência e emergência, mas todos participam de treinamentos e capacitações sobre o AVC ofertadas pela instituição.
Análise das infecções relacionadas ao cateter de diálise.
Authors: Karine Marcomini Emilli 
Published: January 2025

A análise das infecções relacionadas a cateter de diálise é fundamental para conhecimento e redução das taxas de infecção, visto que a insuficiência renal crônica tem destacado enquanto doença crônica não transmissível, assim, objetivo da pesquisa é compreender aspectos das infecções associadas aos cateteres de hemodiálise e identificar medidas de controle de infecção. Dentre a amostra selecionada (n=15), observa-se que os pacientes de diálise são propensos a inúmeras infecções as quais podem ser transmitidas pelo sangue através de agulhas, transfusões e cateteres, que podem potencializar riscos a depender de seu tipo, bem como a contaminação de agentes microbianos. A ocorrência de eventos indesejáveis na assistência, pode ser explicada pela baixa prática de ações seguras de assistência, a qual envolve danos ao próprio paciente, ao serviço de saúde e aos profissionais como um todo. O controle de infecção em cateteres de hemodiálise se inicia com os cuidados na inserção até a manipulação rotineira deste dispositivo, empregando rígidas técnicas assépticas e incluindo qualificação profissional.
Screening and outcomes of co-occurring traumatic brain injury among people with spinal cord injury: a scoping review
Authors: Snell Deborah L. , Wynands Phoebe , Dunn Jennifer , Nunnerley Joanne , Theadom Alice 
Published: January 2025

Objective: To map existing knowledge on screening and rehabilitation outcomes for co-occurring traumatic brain injury among people with traumatic spinal cord injury (SCI).
Methods: Articles focusing on screening and rehabilitation outcomes in participants sustaining co-occurring traumatic brain injury and traumatic spinal cord injury (all ages) were identified in Ovid, Scopus, Web of Science, CINAHL, and ProQuest Dissertations and Theses electronic databases. There were no limitations on study design, date, or geographical location. Articles were excluded if they were not available in English. Data were extracted into the Rayyan online collaboration platform and summarized descriptively.
Results: Twenty-five studies were included, with a mix of retrospective, case-control, and prospective cohort designs. Screening under-estimated traumatic brain injury incidence when approaches relied on inconsistently collected traumatic brain injury indicators, especially for mild traumatic brain injury. Rehabilitation outcomes included length of stay, functional outcomes, cognitive functioning, complication rates, and employment. Although mixed, outcomes among persons with moderate to severe co-occurring traumatic brain injury especially, appeared poorer than those with spinal cord injury alone.
Conclusions: Multivariable approaches to traumatic brain injury ascertainment and greater consistency in documenting acute traumatic brain injury indicators may improve reliability of capturing traumatic brain injury and traumatic brain injury severity among persons with traumatic spinal cord injury. Impacts of co-occurring traumatic brain injury appear greater relative to SCI alone but few studies analysed outcomes based on traumatic brain injury severity.