bluuebunny/crossref_metadata_2025_split huggingface dataset:
Carbohydrate antigen 19-9 (CA19-9) response after induction FOLFIRINOX for locally advanced pancreatic cancer identifies patients who may benefit from surgical exploration: multicentre, observational cohort study
Authors: Dekker Esther N , van Klaveren David , Verkolf Eva M M , de Wilde Roeland F , Besselink Marc G , O’Reilly Eileen M , Paniccia Alessandro , Katz Matthew H G , Tzeng Ching-Wei D , Wei Alice C , Zureikat Amer H , Groot Koerkamp Bas ,   the Trans-Atlantic Pancreatic Surgery (TAPS) Consortium, el Asmar R , DeSilva A , Haviland D , Ilagan C , Janssen Q P , McIntyre S , Prakash L R , Theijse R T 
Published: February 2025

Abstract

Background
Which patients with locally advanced pancreatic cancer may benefit from surgical exploration after induction treatment remains debated. The aim of this retrospective cohort study was to identify independent factors for overall survival after surgical exploration for locally advanced pancreatic cancer, which are available at restaging after induction therapy.

Methods
Consecutive patients with locally advanced pancreatic cancer from the Trans-Atlantic Pancreatic Surgery cohort who underwent surgical exploration after FOLFIRINOX as induction chemotherapy were included. Multivariable Cox proportional hazards analyses with contour plots were used for the predicted 3-year overall survival in patients who underwent surgical exploration.

Results
Overall, 958 patients with locally advanced pancreatic cancer were treated with FOLFIRINOX as induction therapy. At restaging, 221 patients (23.1%) had metastatic disease (M1) and 724 patients (75.6%) did not have metastatic disease (M0)—234 patients (24.4%) with M0 disease underwent surgical exploration and 490 patients (51.1%) with M0 disease did not undergo surgical exploration; restaging information was not available for 13 patients (1%). The surgical exploration cohort included 213 patients with locally advanced pancreatic cancer, after excluding 21 carbohydrate antigen 19-9 non-producers. The independent prognostic factors for overall survival after surgical exploration were post-induction carbohydrate antigen 19-9 level (HR 1.14 (95% c.i. 1.01 to 1.29)), carbohydrate antigen 19-9 decrease (HR 0.89 (95% c.i. 0.79 to 0.99)), and a WHO performance status of greater than or equal to one (HR 1.71 (95% c.i. 1.21 to 2.42)). Baseline carbohydrate antigen 19-9 was not prognostic for overall survival after surgical exploration (HR 0.98 (95% c.i. 0.90 to 1.07)). The best predicted 3-year overall survival was achieved with a greater than 80% decrease in carbohydrate antigen 19-9 and a post-induction carbohydrate antigen 19-9 less than 50 U/ml.

Conclusion
Carbohydrate antigen 19-9 after induction therapy, carbohydrate antigen 19-9 decrease, and performance status are independent prognostic factors for overall survival after surgical exploration for locally advanced pancreatic cancer. Three-year overall survival is best in patients with a performance status of zero, a greater than 80% decrease in carbohydrate antigen 19-9, and a post-induction carbohydrate antigen 19-9 level less than 50 U/ml.
The blood–brain barrier: a help and a hindrance
Authors: Rust Ruslan , Yin Hao , Achón Buil Beatriz , Sagare Abhay , Kisler Kassandra 
Published: February 2025

Abstract
The blood-brain barrier (BBB) is a specialized vascular structure that protects the brain microenvironment from toxins and pathogens in the blood. At the same time, the BBB presents a significant challenge for drug development, as it restricts the entry of most therapeutics into the brain. In this review, we first examine recent findings on the molecular and cellular architecture of the BBB, emphasizing the diverse mechanisms that facilitate the transport and exchange of molecules between the blood and the brain. We discuss current knowledge on the heterogeneity of the BBB along the arteriovenous axis and across different brain regions. Then, we explore the molecular and functional changes in the BBB that occur during normal aging, neurodegeneration, and acute brain injuries and how prolonged BBB impairment affects transport mechanisms and disease progression. Additionally, we discuss novel developments in drug delivery strategies to transport small molecules, biologicals or cellular therapeutics across the BBB. Finally, we examine therapeutic opportunities to restore the BBB and discuss future directions in the field.
The neural characteristics influencing literacy outcome in children with cochlear implants
Authors: Koirala Nabin , Manning Jacy , Neumann Sara , Anderson Chelsea , Deroche Mickael L D , Wolfe Jace , Pugh Kenneth , Landi Nicole , Muthuraman Muthuraman , Gracco Vincent 
Published: February 2025

Abstract
Early hearing intervention in children with congenital hearing loss is critical for improving auditory development, speech recognition, and both expressive and receptive language which translates into better educational outcomes and quality of life. In children receiving hearing aids or cochlear implants (CI), both adaptive and potentially maladaptive neural reorganization can mitigate higher level functions that impact reading. The focus of the present study was to dissect the neural underpinnings of the reading networks in children with cochlear implants and assess how these networks mediate the reading ability in children with cochlear implants.
Cortical activity was obtained using naturalistic stimuli from 75 children (50 cochlear implant recipients, aged 7 to 17, and 25 age-matched children with typical hearing (TH)) using functional Near Infrared Spectroscopy (fNIRS). Assessment of basic reading skill was completed using the Reading Inventory and Scholastic Evaluation (RISE). We computed directed functional connectivity of the hemodynamic activity in reading-associated anterior and posterior brain regions using the time-frequency causality estimation method known as temporal partial directed coherence. The influence of the cochlear implant-related clinical measures on reading outcome and the extent to which neural connectivity mediated these effects were examined using structural equation modelling.
Our findings reveal that the timing of intervention (e.g., age of first cochlear implants, age of first hearing aid) in children with cochlear implants significantly influenced their reading ability. Furthermore, reading related processes (word recognition and decoding, vocabulary, morphology and sentence processing) were substantially mediated by the directed functional connectivity within reading-related neural circuits. Notably, the impact of these effects differed across various reading skills. Hearing age, defined as the age at which a participant received adequate access to sound, and age of initial implantation emerged as robust predictors of reading proficiency.
The current study is one of the first to identify the influence of neural characteristics on reading outcomes for children with cochlear implants. The findings emphasize the importance of duration of deafness and early intervention for enhancing outcomes and strengthening neural network connections. However, the neural evidence further suggested that such positive influences cannot fully offset the detrimental impact of early auditory deprivation. Consequently, additional, perhaps more specialized, interventions might be necessary to maximize the benefits of early prosthetic hearing intervention.
A practical and versatile synthetic strategy for homogeneous polymers of 1,3-propanediamine
Authors: Ohnishi Taiga , Irie Raku , Inai Makoto , Kan Toshiyuki , Oikawa Masato 
Published: February 2025

Abstract
A convergent, iterative, and split-couple strategy for synthesis of homogeneous polymer of 1,3-propanediamine (long-chain polyamine, LCPA) has been developed, by employing 1,3-dibromopropane as a glue in subunit couplings. By this synthetic strategy, 7-, 11-, and 15-mer LCPAs were successfully synthesized. This synthetic strategy solved problems that had arisen with previous methods, such as variable reactivity and poor reproducibility.
The right ventricle: the co-star in the complex history of heart failure
Authors: Manzi Giovanna , Recchioni Tommaso , Mihai Alexandra , Severino Paolo , Birtolo Lucia Ilaria , Filomena Domenico , D’Amato Andrea , Mancone Massimo , Papa Silvia , Badagliacca Roberto , Vizza Carmine Dario 
Published: February 2025

Abstract
The natural history of patients with heart failure (HF), mainly affecting the left ventricle in the initial stages, is marked by the progressive involvement of the right ventricle (RV), which in the advanced stages of the disease becomes dilated and dysfunctional. The geometrical, functional, and pathological interdependence binding the two ventricles underlies this progressive path. Researchers’ and clinicians’ efforts must be aimed at interrupting the inevitable trajectory of HF, by preventing the development of pulmonary hypertension (PH) and RV dysfunction or the transition from isolated post-capillary PH to combined pre- and post-capillary PH. The search for drugs targeting the pulmonary circulation is another goal of PH researchers. Studies conducted so far on drugs currently approved for patients with pulmonary arterial hypertension have yielded contradictory results; an in-depth analysis of these trials could help researchers profile the patients with HF who might benefit from these treatments.
The contemporary role of genetics in cardiovascular medicine: from phenotypes to precision diagnoses
Authors: Urtis Mario , Tagliani Marilena , Bondavalli Davide , Paganini Chiara , Cavaliere Claudia , Vilardo Viviana , Buccieri Edward , Tescari Antonio , Ferrari Michela , Arbustini Eloisa 
Published: February 2025

Abstract
The diagnostic work-up of cardiovascular genetic diseases is emerging as a reference model for precision (cause and phenotype) and personalized medicine (tailored individual management). This approach is expanding across all areas of cardiovascular medicine, ranging from monogenic diseases to multifactorial disorders where ‘risk factors’ such as familial hypercholesterolaemia may play a role in the risk profile. In this context, cardiomyopathies provide an ideal reference because they are monogenic yet genetically heterogeneous diseases, much like many other cardiovascular genetic disorders. In this model, the genetic path starts with deep phenotyping of the patient and relatives and progresses with genetic testing including extensive multigene panels, up to whole-exome sequencing and whole-genome sequencing. Although genetic work-ups are increasingly successful, several unresolved challenges and limitations remain. These include the interpretation and reinterpretation of variants, as many pre-American College of Medical Genetics variants previously classified as likely pathogenic or pathogenic are now recognized as variants of uncertain significance or benign/likely benign; pathogenic variants missed with short-read next generation sequencing (NGS) technologies (e.g. deep intronic variants or Copy Number Variations); gene-specific issues such as pseudogenes and pseudo-exons; and differing interpretations of pathogenicity for the same gene defects by commercial pipelines. Despite widespread NGS-based testing, about half of suspected Mendelian conditions still lack a precise molecular diagnosis. New organizational models are needed to integrate emerging knowledge and innovations incorporating both clinical and genetic data into intelligent platforms that may support shared management pathways.
Global Prevalence of Myocardial Fibrosis among Individuals with Cardiometabolic Conditions: A Systematic Review and Meta-Analysis
Authors: Yeo Tin Mei , Chin Woon Loong Calvin , Seah Chuen Wei Alvin , Cheng Ling Jie , Lin Weiqin , Dalakoti Mayank , Foo Sik Yin Roger , Wang Wenru 
Published: February 2025

Abstract

Background
Cardiometabolic conditions including hypertension, diabetes, hyperlipidaemia and obesity are significant risk factors for cardiovascular diseases. Myocardial fibrosis (MF) is a complication and final common pathway of these conditions, potentially leading to heart failure, arrhythmias and sudden death. Existing reviews explored pathophysiological changes and treatment of MF, but the global prevalence of MF among individuals with cardiometabolic conditions remain limited.

Objectives
To evaluate the global prevalence of MF in individuals with cardiometabolic conditions and explore factors influencing its rate.

Methods
CINAHL, Cochrane Library, Embase, PubMed, ProQuest Theses and Dissertations, Scopus, and Web of Science were systematically reviewed until January 2024. Studies included individuals with hypertension, type 2 diabetes mellitus, hyperlipidaemia, and obesity, with MF prevalence assessed via biopsy or Late Gadolinium Enhancement-Cardiac Magnetic Resonance (LGE-CMR). Meta-analysis was conducted using jamovi and factors associated with MF were synthesised narratively. This review is registered on PROSPERO, CRD42024544632.

Results
The meta-analysis included 52 articles involving 5,921 individuals. 32.7% of individuals with cardiometabolic conditions developed MF, with hypertension demonstrating the highest prevalence [35.2%(95%CI:25.5-45.0)]. Biopsy-based studies reported a higher prevalence [75.6%(95%CI:53.6-97.6)] compared to LGE-CMR studies [26.8%(95%CI:20.6-33.0)]. Key factors associated with MF included increased LV mass/LV hypertrophy, reduced LV function, and myocardial stiffness.

Conclusions
This first global review estimates that one-third of individuals with cardiometabolic conditions develop MF, with the rate expected to rise. Standardized CMR measures cut-offs are needed to address prevalence inconsistencies. Future research should explore MF prevalence using diverse samples, combined CMR measures, considering socio-demographic and clinical factors for more accurate estimates.
Outdoor Environmental Risk Factors for Falls and Fear of Falling: A Systematic Review
Authors: Dunlap Pamela M , Fan Erica K , Hubbard Zachary L , VonVille Helena M , Strotmeyer Elsa S , Rosso Andrea L 
Published: February 2025

Abstract

Background and Objectives
The objective of this systematic review was to determine the outdoor environmental factors that are associated with increased risk of falls and fear of falling in middle-aged and older adults.

Research Design and Methods
PubMed, Medline, EMBASE, APA Psych INFO, and CINAHL electronic databases were searched through August 2024 for studies that included participants 45 years or older, falls or fear of falling as an outcome, and measure the outdoor environment (i.e., uneven sidewalks, neighborhood disorder). Title and abstract screenings, full text screenings, and critical appraisal assessments were independently reviewed.

Results
The search retrieved 7474 records, 627 full texts were reviewed, and 51 unique studies met the inclusion/exclusion criteria. The number of participants ranged from 12 to 6,720,937. There were 41 studies that included fall outcomes, 5 studies that included fear of falling outcomes, and 5 studies that included both outcomes. Micro-walkability (e.g., sidewalk quality) were the most frequently studied outdoor environmental characteristics and many studies found associations with falls and fear of falling. Macro-walkability (e.g., land use), crime and safety, socioeconomic, greenspace, terrain, physical disorder, and social features were studied to a lesser extent and had mixed findings.

Discussion and Implications
The most consistent associations were found between micro-walkability features of the outdoor environment with falls whereas other environmental domains and fear of falling were studied to a lesser extent. Environmental contributors to falls should be considered in future research, policy implementation, and clinical interventions to reduce falls and their negative outcomes for older adults.
The minimum information required for a glycomics experiment (MIRAGE) project: improving the standards for reporting lectin microarray data
Authors: Tateno Hiroaki , Mahal Lara K , Feizi Ten , Kettner Carsten , Paulson James C 
Published: February 2025

Abstract
The MIRAGE (Minimum Information Required for a Glycomics Experiment) project has been established by experts in glycobiology, glycoanalytics, and glycoinformatics under the auspieces of the Beilstein-Institut. The working group aims to develop guidelines for reporting results from various experiments and analyses conducted in structural and functional studies of glycans in the scientific literature. Previous guidelines have been established for glycomic analytics, including mass spectrometry and glycan microarrays. Lectin microarrays are used worldwide for glycan profiling of various biological samples, but there are often insufficient reports on information about experimental methods such as sample preparation and fluorescence labeling. Here, we propose guidelines specifically designed to improve the standards for reporting data from lectin microarray analyses. For each of the seven areas in the workflow of a lectin microarray experiment, we provide recommendations for the minimum information that should be included when reporting results. When adopted by the scientific community the MIRAGE lectin microarray guidelines are expected to enhance data interpretation, facilitate comparison of data between laboratories and encourage the deposition of lectin microarray data in international databases.
Intellectual community in Saint Victor: 1108–c.1200
Authors: Hopper Joseph 
Published: February 2025

Abstract
This article examines the community of Saint Victor in the twelfth century and argues the dangers of considering intellectual life and communal life as two distinct things. It responds critically to the tendency to separate the two by canalizing intellectual activities into the model of a ‘school’. This article suggests instead the importance of thinking of Saint Victor as an ‘intellectual community’. The immediate significance for Victorine studies lies in thinking more imaginatively about the relationship between the ‘ideas’ which are rightly famous and the community which remains strangely anonymous. Beyond Saint Victor, this approach disrupts a much broader historiographical landscape of ‘cathedral schools’, ‘the university’ and twelfth-and-thirteenth-century Paris. What emerges is a city that played host to experimentation, both in ideas and the ways that communal life could be organized to facilitate their exchange and development.
Performance Validation of the IEH Laboratories Multiplex PCR Listeria Detection System for Environmental Samples in Comparison to Health Canada Reference Method (MFHPB-30) for Listeria Detection
Authors: Themeli Eni , Mai Tam , Forghani Fereidoun , Samadpour Mansour 
Published: February 2025

Abstract

Background
Laboratory detection methods are commonly tested against gold standards and corresponding reference methods to confirm their suitability and efficiency.

Objective
The IEH Listeria Test System (multiplex PCR) was tested against Health Canada’s reference method (MFHPB-30) for the simultaneous detection of Listeria spp. and Listeria monocytogenes, on three different surfaces: plastic (PL), sealed concrete (SC) and stainless steel (SS).

Methods
The R2 medium was used for pre-enrichment of the samples at 35 °C for 24 h, followed by the IEH Listeria multiplex PCR. Each individual surface coupon (PL, SC, SS) was inoculated with either Listeria innocua, L. monocytogenes, or L. welshimeri along with two non-target microorganisms of concern and subjected to candidate (IEH Listeria multiplex PCR) and reference method (MFHPB-30) testing in an unpaired manner, and included culture confirmation for IEH method.

Results
The candidate method demonstrated 0% false negative, 0% false positive, 100% sensitivity, and 100% specificity after only 24 h enrichment followed by Multiplex PCR.

Conclusion
Evaluating the differences between the candidate and reference method detection probabilities across all three surface types revealed that the candidate method was equivalent to MFHPB-30, while requiring significant less time for detection.
Identification and characterization of a novel hemolytic and hemagglutinating bifunctional lectin from the coral Acropora millepora
Authors: Takahashi Yuki , Kamimura Ryosuke , Toyama Ryo , Kita Shun , Ushijima Yuki , Taniyama Shigeto , Unno Hideaki , Hatakeyama Tomomitsu , Goda Shuichiro 
Published: February 2025

Abstract
Two genes, AML-I and AML-II, have been reported to exhibit increased expression during the development of the coral Acropora millepora. They show amino acid sequence homology with CEL-III, a hemolytic lectin found in the sea cucumber Cucumaria echinata. CEL-III binds to carbohydrate chains on the surface of erythrocytes, forming heptameric pores in their membranes. To clarify the role of these proteins in coral, we identified and elucidated their functions. The carbohydrate binding domains of them showed similar carbohydrate-binding specificity as that of CEL-III. AML-I showed hemagglutinating activity in erythrocytes, whereas AML-II can only be prepared as an aggregate and its function could not yet be determined. AML-IΔC and AML-IIΔC mutants were generated through deletion of the C-terminal extended amino acid residues of them relative to CEL-III. AML-IΔC showed hemolytic activity toward erythrocytes, whereas AML-IIΔC showed no activity. A tobacco etch virus (TEV) protease recognition site was inserted into the C-terminus of AML-I to regulate these activities. The hemagglutinating activity of AML-I was converted into hemolytic activity after TEV protease treatment. As a result, TEV protease could control the hemolytic and hemagglutinating activity of the lectin, which could be useful as an anticancer or antiviral drug because of its cytotoxic activity.
A Critical Interpretive Literature Review of Phronesis in Medicine
Authors: Jameel Sabena Yasmin 
Published: February 2025

Abstract
This article presents the results of a rigorous critical interpretive review that maps the current literature on phronesis in medicine. The literature in this area involves varied disciplines, centuries, and conceptions and is extensive, but through a focused review, this study seeks to clarify definitions and key distinctions. It thereby aims to elucidate a depth of meaning and understanding regarding phronesis in medicine to inform future work on the topic. Specifically, 12 themes are inductively identified and analyzed from the literature, and organized into three chronological categories of past, present, and future. A narrative summation of the literature to date is then offered, assessing the varied conceptual applications of phronesis to medical practice, the emerging literature on its applicability to organizations, prospects for empirical work on the concept, and its application in medical education.