bluuebunny/crossref_metadata_2025_split huggingface dataset:
Reference intervals for selected haematological and biochemical parameters among apparently healthy adults in different eco-geographical zones in Ghana
Authors: Abbam Gabriel , Tandoh Samuel , Tetteh Mary , Afrifah David Amoah , Annani-Akollor Max Efui , Owiredu Eddie-Williams , Gyasi Charles , Adu-Gyamfi Constance , Sackey Benedict , Debrah Alexander Yaw , Addai-Mensah Otchere 
Published: January 2021

Background
Due to the influence of gender, race/genetics, age, lifestyle habits and geography on the references intervals (RIs), the Clinical and Laboratory Standards Institute (CLSI) recommends the determination of population-specific RIs. Ghana continues to depend on pre-established RIs from other countries which poses the risk of misdiagnoses and wrong treatment. This study presents the haemato-biochemical RIs from four eco-geographical zones in Ghana.

Methods
In this population-based cross-sectional study, a total of 1227 randomly selected healthy voluntary blood donors from the four eco-geographic zones (Coastal Savannah, Rain Forest, Savannah and Transitional) were enrolled and screened. Based on the CLSI Guidance Document C28A2992, the data of eligible participants were used to non-parametrically determine the RIs for the haemato-biochemical parameters at the 2.5th and 97.5th percentiles. Comparison of analytes by gender was done by Wilcoxon rank sum test and eco-geographic differences were assessed using the Kruskal-Wallis with the Dunn post hoc multiple comparison tests.

Results
There were statistically significant differences in most of the haematological parameters (RBC, Hb, HCT, MCV, PLT, WBC; p-values <0.0001 and MCH; p-value = 0.007), and biochemical analytes (Urea, Cr, Trig, HDL-C, AST, ALT, ALP, GGT, BID, BIT, Prot-T and Albumin; p-values <0.0001) based on gender. Significant inter eco-geographic (intra-population) variations and substantial differences between the established RI and the RIs accompanying the analyzers used were also observed.

Conclusion
This study reports significant inter-sex and inter-geographical differences in haemato-biochemical RIs in Ghana as well as differences in RIs with both the RIs accompanying the analyzers and those of other countries. Determining RIs representative of populations and including them in the report systems of laboratories to ensure effective and efficient healthcare service delivery is thus recommended.
Urgency urinary incontinence, loss of independence, and increased mortality in older adults: A cohort study
Authors: Yoshioka Takashi , Kamitani Tsukasa , Omae Kenji , Shimizu Sayaka , Fukuhara Shunichi , Yamamoto Yosuke 
Published: January 2021

Objectives
To investigate the longitudinal association of urgency urinary incontinence (UUI) with loss of independence (LOI) or death among independent community-dwelling older adults.

Design
Population-based cohort study.

Setting
The Locomotive Syndrome and Health Outcome in Aizu Cohort Study (LOHAS), Minami-Aizu Town and Tadami Town, Fukushima, Japan.

Participants
A total of 1,580 participants aged ≥65 years who underwent a health check-up conducted by LOHAS in 2010.

Measurements
Exposure was defined as the presence of UUI, which was measured by a questionnaire based on the definition of UUI from the International Continence Society. The primary outcome was defined as incidence of LOI or death. After the check-up in 2010, the outcome was monitored until March 2014. A multivariable Cox proportional hazard analysis was performed to estimate the hazard ratio for the outcome. Ten potential confounders were adjusted in the analysis. Furthermore, we defined the secondary outcomes as two separate outcomes, LOI and death, and performed the same analysis.

Results
Among all participants, 328 reported UUI. The incidence rates of the outcome were 20.4 and 11.4 (per 1,000 person–years) among participants with and without UUI, respectively. After multivariable adjustment, those who experienced UUI showed a substantial association with LOI or death (HR, 1.65; 95% CI, 1.01–2.68). However, they did not show such an association with LOI alone (HR, 1.07; 95% CI, 0.49–2.33). On the other hand, those with UUI exhibited a substantial association with death (HR, 2.23; 95% CI, 1.22–4.31).

Conclusions
In this study, UUI was associated with the occurrence of LOI or death; however, UUI is not associated with the occurrence of LOI alone among independent community-dwelling older adults. Our results suggest that there may be a difference between UUI-associated diseases that cause LOI and those that cause death.
Platypnea-Orthodeoxia Syndrome Caused by an Intracardiac Shunt
Authors: Borgaonkar Sanket P. , Lam Wilson W. , Razavi Mehdi , Parekh Dhaval R. 
Published: January 2021

Platypnea-orthodeoxia syndrome, a rare condition characterized by posture-related dyspnea, is usually caused by an intracardiac shunt, hepatopulmonary syndrome, or shunting resulting from severe pulmonary disease.
We report the case of a 33-year-old woman who presented with increasing dyspnea and oxygen desaturation when she sat up or arose. Our diagnosis was platypnea-orthodeoxia syndrome. A lead of a previously implanted pacemaker exacerbated a severe tricuspid regurgitant jet that was directed toward the patient's intra-atrial septum. Percutaneous closure of a small secundum atrial septal defect eliminated right-to-left shunting and substantially improved the patient's functional status. In addition to this case, we discuss this unusual condition.
Importance of Persistent Right-to-Left Shunt After Patent Foramen Ovale Closure in Cryptogenic Stroke Patients
Authors: He Lu , Cheng Gesheng , Du Yajuan , Zhang Yushun 
Published: January 2021

Percutaneous closure of patent foramen ovale (PFO) is widely performed to prevent recurrent stroke or transient ischemic attack in patients with cryptogenic stroke. However, the influence of different degrees of right-to-left shunting (RLS) has rarely been reported.
We retrospectively evaluated the cases of 268 patients with cryptogenic stroke who underwent PFO closure at our hospital from April 2012 through April 2015. In accordance with RLS severity, we divided the patients into 2 groups: persistent RLS during normal breathing and the Valsalva maneuver (n=112) and RLS only during the Valsalva maneuver (n=156). Baseline characteristics, morphologic features, and procedural and follow-up data were reviewed. The primary endpoint was stroke or transient ischemic attack.
More patients in the persistent group had multiple or bilateral ischemic lesions, as well as a larger median PFO diameter (2.5 mm [range, 1.8–3.9 mm]) than did patients in the Valsalva maneuver group (1.3 mm [range, 0.9–1.9 mm]) (P <0.001). Atrial septal aneurysm was more frequent in the persistent group: 25 patients (22.3%) compared with 18 (11.5%) (P=0.018). Three patients in the persistent group had residual shunting. The annual risk of recurrent ischemic stroke was similar between groups: 0.298% (persistent) and 0.214% (Valsalva maneuver).
Our findings suggest that patients with persistent RLS have more numerous severe ischemic lesions, larger PFOs, and a higher incidence of atrial septal aneurysm than do those without. Although our persistent group had a greater risk of residual shunting after PFO closure, recurrence of ischemic events did not differ significantly from that in the Valsalva maneuver group.
En Bloc AngioVac Removal of Thoracic Aortic Mass
Authors: Kang Jeanney , Fleischman Fernando , Saremi Farhood , Shavelle David M. 
Published: January 2021

The AngioVac system, designed for suction during extracorporeal bypass, is used to aspirate masses, thrombi, and other undesirable material from the cardiovascular system. To date, it has been used extensively in the venous system and right side of the heart; however, its use in the arterial system has been limited because of smaller vessel sizes and the requirement for a 26F sheath.
We report the case of a 45-year-old woman with a history of angiosarcoma who presented with acute embolic events that affected her spleen and lower extremities. We removed a large mobile mass en bloc from her distal thoracic aorta by using the AngioVac system as an alternative to surgical resection. The patient recovered with no recurrence. We discuss the benefits and challenges of using the AngioVac within small vessels of the arterial system.
Mechanical Prosthetic Valve Sparing for Aortic Root Abscess Complicated by Infective Endocarditis
Authors: Ahmed Ahmed , Ammar Ayman , Elnahas Yasser , Abd Al Jawad Mohammed 
Published: January 2021

Aortic root abscess complicated by infective endocarditis of a mechanical prosthetic valve is associated with morbidity and death. We retrospectively report our experience with a valve-sparing technique for managing this condition.
From October 2014 through November 2017, 41 patients at our center underwent surgery for aortic root abscess complicated by infective endocarditis of a mechanical prosthetic valve. Twenty (48.7%) met prespecified criteria for use of our valve-sparing technique after careful assessment of the mechanical valve and surrounding tissues. Our technique involved draining the abscess, aggressively débriding all infected and necrotic tissues, and then repairing the resulting defect by suturing a Gelweave patch to the healthy aortic wall and to the cuff of the valve.
We successfully preserved the mechanical aortic valve in all 20 patients. Two (10%) died early (≤30 d postoperatively) of low cardiac output syndrome with progressive heart failure, superadded septicemia, and multisystem organ failure. At 1-year follow-up, the 18 surviving patients (90%) were symptom free and had a well-functioning mechanical aortic valve with no paravalvular leak.
We conclude that, in certain patients, our technique for managing aortic root abscess and sparing the mechanical aortic valve is a safe and less time-consuming approach with relatively low mortality and encouraging midterm follow-up outcomes.
Validity and Reliability of a Snatch Pull Test to Model the Force-Velocity Relationship in Male Elite Weightlifters
Authors: Sandau Ingo , Chaabene Helmi , Granacher Urs 
Published: January 2021

Abstract
Sandau, I, Chaabene, H, and Granacher, U. Validity and reliability of a snatch pull test to model the force-velocity relationship in male elite weightlifters. J Strength Cond Res 36(10): 2808–2815, 2022—This study examined the concurrent validity and within-session reliability of parameters describing the force-velocity relationship (FvR) such as maximal force, velocity, power, and the theoretical one repetition maximum snatch performance (snatch
th) during the snatch pull. The FvR was assessed using the multiple-load (FvRm) approach and the 2-load (FvR2) approach. Eight male elite weightlifters from the German national team executed the snatch pull in 2 separate experiments. For the concurrent validity assessment (experiment one), during the snatch pull, 7 loads from 70 to 100% were lifted to compute the FvRm, and 2 loads (70 and 100%) were lifted to compute the FvR2. For the reliability assessment (experiment 2), a test-retest protocol for the FvR2 was conducted. Input FvR parameters were determined from video-based barbell tracking. Results indicated no differences (all p > 0.05; all d ≤ 0.07) and extremely large correlations (all r ≥ 0.91) between the FvRm and FvR2 parameters. The within-session reliability of FvR2 parameters was excellent (all intraclass correlation coefficient ≥0.97; SEM% ≤1.23%). The percentage smallest real difference (SRD95%) of FvR2 parameters ranged between 1.89 and 3.39%. In summary, using the snatch pull to model FvR2 parameters is a valid and reliable approach that can easily be integrated into elite weightlifters' daily training routines.
Beyond COVID-19: Preparing Fitness Centers for the Next Pandemic
Authors: Armstrong William Jeffrey , Dayal Nishu N. , Braun William A. 
Published: January 2021

ABSTRACT
The 2019 novel coronavirus (COVID-19) has caused governments to institute measures to curb the spread of the virus. Among these were restrictions that affect the daily operations of public gymnasiums, personal training centers, therapy clinics, strength and conditioning facilities, and the like. As the world has seen widespread public health crises with 3 beta-coronaviruses, as well as highly contagious strains of type A influenza (H5N1 and H1N1), in the past 2 decades, operators of fitness facilities are challenged to consider how daily operations will be affected in the wake of COVID-19. This article examines strategies to mitigate the transmission of the virus in fitness facilities, including temperature scanning, physical distancing, masks, hand hygiene, and the disinfection of large spaces and common touch surfaces. Fitness facilities and therapy centers face complex challenges in minimizing the risk of viral transmission due to space limitations and the use of common equipment and spaces by clients. Despite these challenges, the developing science resulting from the COVID-19 pandemic has led to insights that should drive policies to help ensure fitness facilities successfully adapt to the risks presented by these infectious agents.
Thalidomide and its metabolite 5‐hydroxythalidomide induce teratogenicity via the cereblon neosubstrate PLZF
Authors: Yamanaka Satoshi , Murai Hidetaka , Saito Daisuke , Abe Gembu , Tokunaga Etsuko , Iwasaki Takahiro , Takahashi Hirotaka , Takeda Hiroyuki , Suzuki Takayuki , Shibata Norio , Tamura Koji , Sawasaki Tatsuya 
Published: January 2021

AbstractThalidomide causes teratogenic effects by inducing protein degradation via cereblon (CRBN)‐containing ubiquitin ligase and modification of its substrate specificity. Human P450 cytochromes convert thalidomide into two monohydroxylated metabolites that are considered to contribute to thalidomide effects, through mechanisms that remain unclear. Here, we report that promyelocytic leukaemia zinc finger (PLZF)/ZBTB16 is a CRBN target protein whose degradation is involved in thalidomide‐ and 5‐hydroxythalidomide‐induced teratogenicity. Using a human transcription factor protein array produced in a wheat cell‐free protein synthesis system, PLZF was identified as a thalidomide‐dependent CRBN substrate. PLZF is degraded by the ubiquitin ligase CRL4CRBN in complex with thalidomide, its derivatives or 5‐hydroxythalidomide in a manner dependent on the conserved first and third zinc finger domains of PLZF. Surprisingly, thalidomide and 5‐hydroxythalidomide confer distinctly different substrate specificities to mouse and chicken CRBN, and both compounds cause teratogenic phenotypes in chicken embryos. Consistently, knockdown of Plzf induces short bone formation in chicken limbs. Most importantly, degradation of PLZF protein, but not of the known thalidomide‐dependent CRBN substrate SALL4, was induced by thalidomide or 5‐hydroxythalidomide treatment in chicken embryos. Furthermore, PLZF overexpression partially rescued the thalidomide‐induced phenotypes. Our findings implicate PLZF as an important thalidomide‐induced CRBN neosubstrate involved in thalidomide teratogenicity.
Distribution of adventive species Solidago canadensis, Phalacroloma annuum, Ambrosia artemisiifolia, Heracleum sosnowskyi in phytocenoses of Volyn’ Polissya (Ukraine)
Authors: Oitsius L. V. , Volovyk H. P. , Doletskyі S. P. , Lysytsya A. V. 
Published: January 2021

Biological pollution of natural phytocenoses by adventive plant species poses is a serious threat to endemic species and species with narrower ecological amplitude in ecosystems around the world. This study presents the results of a study of the composition and distribution of adventive plant species in natural, semi-natural and anthropogenic transformed phytocenoses of Volyn’ Polissya, Ukraine. To clarify the effect of drainage melioration on non-native flora species distribution, a botanical study was carried out during the 2003–2019 vegetation seasons on the territory of four drainage systems. The adventive flora of this unique region of Europe was studied in detail for the first time. In total, 279 non-native plant species were found. They belong to 110 genera and 32 families. The results of studying the systematic, bioecological, range-distributional and phytogenetic structure of adventive species found within agricultural lands and adjacent territories are presented. It was found that 161 species are associated with agricultural production. Of these, 90 species were found directly within the agricultural land, another 71 species were found growing in the adjacent territories. It was found that a significant increase in the number of adventive species on the territory of Volyn’ Polissya is associated with drainage melioration carried out in the 1960–1990s, significant changes in the structure and forms of agro-industrial production, and climatic changes in recent decades. The expansion of agricultural land in this area over the past 50–60 years has led to an increase in the number of adventive species by more than 60%. On the territory of drainage systems used for agricultural activities, more than 40% of the total species composition of the adventive flora of Volyn’ Polissya is represented. The majority of these species originate from arid and sub arid regions of the planet. The transformer plants, Solidago canadensis L. and Phalacroloma annuum (L.) Dumort pose a potential threat to phytocenoses of the described region, as well as the whole of Europe. Of particular interest are the species that in the future may pose a serious threat to natural phyto-diversity and have negative practical consequences for the structure of agricultural landscapes. These are Ambrosia artemisiifolia L. and Heracleum sosnowskyi Manden. In general, modern agro ecosystems are characterized by instability and low ability to resist non-native species. The strongly weedy character of cultivated fields and the presence of abandoned uncultivated lands have caused the rapid spread of adventive vegetation. In order to further optimize the structure of agrolandscapes, it is advisable to monitor and regulate not only expansionary invasive species, which is especially important for preventing biological pollution, but also species whose status has not yet been determined.
Patterns of vegetation succession in abandoned fields in semi-arid conditions
Authors: Borovyk L. P. 
Published: January 2021

Successions in old fields were studied on the example of the Starobilski steppes within Luhansk Oblast (Ukraine), region located in the basin of the left tributaries of the Siversky Donets, in the southern spurs of the Central Russian Upland. Stationary surveys were conducted in the Striltsivsky Steppe affiliate of the Luhansk Nature Reserve, with 269 ha of old fields (26% of the area) in its territory. Geobotanical releves were performed in 26 plots of abandoned fields, constant monitoring surveys were carried out in 15–27 year-old abandoned fields in the territory of the Reserve and 5–15 year-old abandoned fields in the territory of its buffer zone. In total, the itinerary and detailed surveys were conducted for about 50 plots of abandoned fields. The restoration of the steppe communities was seen only in the plots where grazing and/or mowing took place, which prevented the formation of dense litter and distribution of woody species. The long domination of rhizome grasses (Elytrigia repens) were characteristic in conditions of moderate-level disturbance (in the reserve), transition to the domination of bunchgrasses (Festuca valesiaca) was seen at the age of around 25 years, during the transitional period (20–25 years) communities with unstable structure formed, comprising E. repens, F. valesiaca and forbs (Achillea pannonica and Fragaria viridis). Duration of the stage of segetal and ruderal species in the sites under low effect was 10 years, the communities of unstable structure formed at the age of 5–10 years. In the conditions of stable moderate and high effect of grazing (high disturbance), we observed formation of bunchgrass communities at the age of 15 years. In the unused plots, we saw formation of phytocoenoses of shrubs at different stages of succession, starting from 10 years. The most abundant group of such kind comprised thickets of Ulmus pumila (with E. repens and Poa angustifolia in the herbaceous layer) and Prunus stepposa. The peculiarity of contemporary processes in the abandoned fields related to exacerbation of the vegetation’s anthropogenic transformation is distribution of alien tree species from plantations, the most aggressive of which are Ulmus pumila, Fraxinus lanceolata, Acer negundo, Elaeagnus angustifolia. The peculiarity of the succession processes in the region of studies was the domination of species of broad ecological spectrum, characteristic for abandoned fields of northern forest-steppe regions (Bromopsis inermis, Calamagrostis epigeios, Poa angustifolia, Fragaria viridis) and southern steppe regions (Bromus sguarrosus, Anisantha tectorum, Achillea pannonica, Artemisia austriaca), certain communities form dependent on the climatic conditions and type of land use in the period of succession in particular plots.
Taxonomy of the wild species of genus Crataegus (Rosaceae): An updated review for the flora of Nakhchivan Autonomous Republic (Azerbaijan)
Authors: Ibrahimov A. M. , Matsyura A. V. , Jankowski K. 
Published: January 2021

We clarified the current state of genus Crataegus L. in the flora of the Nakhchivan Autonomous Republic (Azerbaijan) using a comparative analysis of herbarium specimens from the Azarbaijan herbariums and data collected during the expeditions in 2004–2018. We succeed in adding some important data on the flora of Nakhchivan Autonomous Republic and Azerbaijan, we also enhanced the previous research with chromosome and morphological analysis. We identified 17 wild species of Crataegus genus in the Nakhchivan Autonomous Republic (C. pentagyna Waldst. et Kit. ex Willd., 1800, C. zangezura Pojark., 1939, C. orientalis Pall. ex Bieb., 1808, C. pojarkoviae Kossych, 1964, C. tournefortii Griseb., 1843, C. szovitsii Pojark., 1939, C. pontica C. Koch, 1853, C. meyeri Pojark., 1939, C. eriantha Pojark., 1939, C. atrosanguinea Pojark., 1939, C. caucasica C. Koch, 1853, C. pallasii Griseb., 1843, C. rhipidophylla Gand. (С. curvisepala Lindm.; C. kyrtostyla Pojark.) 1871, C. pseudoheterophylla Pojark., 1939, C. monogyna Jacq., 1775, C. x armena Pojark., 1939, and C. x cinovskisii Kassumova), which will contribute to five introduced cultural species used in the greenery. We also clarified the status of interserial and intersection hybrids using the chromosome and morphological analysis. The article includes the complete list of Crataegus L. species with information about synonyms of the species, patterns of distribution, habitat, latitude and elevation, flowering and fruiting phenology.