bluuebunny/crossref_metadata_2025_split huggingface dataset:
An insight into the quality of sacred groves – an island habitat – using leaf-litter ants as an indicator in a context of urbanization
Authors: Rajesh T.P. , Unni Anjana P. , Prashanth Ballullaya U. , Manoj K. , Sinu Palatty Allesh 
Published: June 2021

AbstractSacred groves (SGs) of India are islets of forests providing ecosystem and spiritual services to man. Studies suggest that SGs are deteriorating on their quality due to urbanization, invasive species, land-use change, and religious modernization. We explored diversity, community, and abundance of overall and different functional groups of litter ants, including Anoplolepis gracilipes – an invasive ant – on paired SG-neighbouring home garden (HG) sites in rural and urban landscape to (a) assess the quality of SGs and (b) examine whether the variation in ant community of the two habitats was predicted by urbanization and abundance of A. gracilipes. We considered species and local contribution to β-diversity to identify species and sites crucial for conservation of sites. Abundance and richness of overall ants, proportional trap incidence of species, and abundance of A. gracilipes were similar on SG and HG, but species diversity and abundance of certain ant functional groups were higher on SG. Ant community of SG was different from HG, but was not affected by urbanization. A. gracilipes and rural SGs contributed the most to β diversity. A. gracilipes gave little pressure on native ant community. The study concludes that SGs, despite invaded by A. gracilipes, have potential for conserving biodiversity.
Hippocampal Volumes in Amnestic and Non-Amnestic Mild Cognitive Impairment Types Using Two Common Methods of MCI Classification
Authors: Emmert Natalie A. , Reiter Katherine E. , Butts Alissa , Janecek Julie K , Agarwal Mohit , Franczak Malgorzata , Reuss James , Klein Andrew , Wang Yang , Umfleet Laura Glass 
Published: June 2021

AbstractObjectives:Mild cognitive impairment (MCI) types may have distinct neuropathological substrates with hippocampal atrophy particularly common in amnestic MCI (aMCI). However, depending on the MCI classification criteria applied to the sample (e.g., number of abnormal test scores considered or thresholds for impairment), volumetric findings between MCI types may change. Additionally, despite increased clinical use, no prior research has examined volumetric differences in MCI types using the automated volumetric software, Neuroreader™.Methods:The present study separately applied the Petersen/Winblad and Jak/Bondi MCI criteria to a clinical sample of older adults (N= 82) who underwent neuropsychological testing and brain MRI. Volumetric data were analyzed using Neuroreader™ and hippocampal volumes were compared between aMCI and non-amnestic MCI (naMCI).Results:T-tests revealed that regardless of MCI classification criteria, hippocampal volumez-scores were significantly lower in aMCI compared to naMCI (p’s < .05), and hippocampal volumez-scores significantly differed from 0 (Neuroreader™ normative mean) in the aMCI group only (p’s < .05). Additionally, significant, positive correlations were found between measures of delayed recall and hippocampalz-scores in aMCI using either MCI classification criteria (p’s < .05).Conclusions:We provide evidence of correlated neuroanatomical changes associated with memory performance for two commonly used neuropsychological MCI classification criteria. Future research should investigate the clinical utility of hippocampal volumes analyzed via Neuroreader™ in MCI.
Predicting Water Temperature Dynamics of Unmonitored Lakes With Meta‐Transfer Learning
Authors: Willard Jared D. , Read Jordan S. , Appling Alison P. , Oliver Samantha K. , Jia Xiaowei , Kumar Vipin 
Published: June 2021

AbstractMost environmental data come from a minority of well‐monitored sites. An ongoing challenge in the environmental sciences is transferring knowledge from monitored sites to unmonitored sites. Here, we demonstrate a novel transfer‐learning framework that accurately predicts depth‐specific temperature in unmonitored lakes (targets) by borrowing models from well‐monitored lakes (sources). This method, meta‐transfer learning (MTL), builds a meta‐learning model to predict transfer performance from candidate source models to targets using lake attributes and candidates' past performance. We constructed source models at 145 well‐monitored lakes using calibrated process‐based (PB) modeling and a recently developed approach called process‐guided deep learning (PGDL). We applied MTL to either PB or PGDL source models (PB‐MTL or PGDL‐MTL, respectively) to predict temperatures in 305 target lakes treated as unmonitored in the Upper Midwestern United States. We show significantly improved performance relative to the uncalibrated PB General Lake Model, where the median root mean squared error (RMSE) for the target lakes is 2.52°C. PB‐MTL yielded a median RMSE of 2.43°C; PGDL‐MTL yielded 2.16°C; and a PGDL‐MTL ensemble of nine sources per target yielded 1.88°C. For sparsely monitored target lakes, PGDL‐MTL often outperformed PGDL models trained on the target lakes themselves. Differences in maximum depth between the source and target were consistently the most important predictors. Our approach readily scales to thousands of lakes in the Midwestern United States, demonstrating that MTL with meaningful predictor variables and high‐quality source models is a promising approach for many kinds of unmonitored systems and environmental variables.
Phosphatidylserine externalized on the colonic capillaries as a novel pharmacological target for IBD therapy
Authors: Zhang Xuerui , Song Lulu , Li Lin , Zhu Banghui , Huo Lina , Hu Zhaoqing , Wang Xinran , Wang Jie , Gao Mengyue , Zhang Jing , Hua Zichun 
Published: June 2021

AbstractInflammatory bowel disease (IBD) is a chronic and relapsing disorder for many people associated with poor health. Although there are some clinical drugs for IBD treatment, the development of effective therapeutics on IBD patients has always been necessary. Here, we show that externalized phosphatidylserine (PS) is observed on the surface of colonic capillaries. Annexin A5 (ANXA5) with high affinity for PS has a good targeting to the colon and effectively alleviates experimental colitis. In contrast, ANXA5 mutant (A5m) lacking the PS-binding ability, has no accumulation in the colon and no therapeutic effects on colitis. Mechanistic investigations indicate that ANXA5 reduces the inflammatory cell infiltration by inhibiting endothelial cell activation dependent on PS-binding ability. With the increasing of PS exposure on activated HUVECs (human umbilical vein endothelial cells), ANXA5 binding induces the internalization of TLR4 via PS-dependent endocytosis. We provide new insights on the molecular mechanism of ANXA5 for its anti-inflammatory effect. Our data suggest that PS-externalization is a potential target of ANXA5 aiming at targeted drug delivery (TDD) for IBD treatment.
Clobetasol promotes neuromuscular plasticity in mice after motoneuronal loss via sonic hedgehog signaling, immunomodulation and metabolic rebalancing
Authors: Vicario Nunzio , Spitale Federica M. , Tibullo Daniele , Giallongo Cesarina , Amorini Angela M. , Scandura Grazia , Spoto Graziana , Saab Miriam W. , D’Aprile Simona , Alberghina Cristiana , Mangione Renata , Bernstock Joshua D. , Botta Cirino , Gulisano Massimo , Buratti Emanuele , Leanza Giampiero , Zorec Robert , Vecchio Michele , Di Rosa Michelino , Li Volti Giovanni , Lazzarino Giuseppe , Parenti Rosalba , Gulino Rosario 
Published: June 2021

AbstractMotoneuronal loss is the main feature of amyotrophic lateral sclerosis, although pathogenesis is extremely complex involving both neural and muscle cells. In order to translationally engage the sonic hedgehog pathway, which is a promising target for neural regeneration, recent studies have reported on the neuroprotective effects of clobetasol, an FDA-approved glucocorticoid, able to activate this pathway via smoothened. Herein we sought to examine functional, cellular, and metabolic effects of clobetasol in a neurotoxic mouse model of spinal motoneuronal loss. We found that clobetasol reduces muscle denervation and motor impairments in part by restoring sonic hedgehog signaling and supporting spinal plasticity. These effects were coupled with reduced pro-inflammatory microglia and reactive astrogliosis, reduced muscle atrophy, and support of mitochondrial integrity and metabolism. Our results suggest that clobetasol stimulates a series of compensatory processes and therefore represents a translational approach for intractable denervating and neurodegenerative disorders.
Osteocrin, a novel myokine, prevents diabetic cardiomyopathy via restoring proteasomal activity
Authors: Zhang Xin , Hu Can , Yuan Xiao-Pin , Yuan Yu-Pei , Song Peng , Kong Chun-Yan , Teng Teng , Hu Min , Xu Si-Chi , Ma Zhen-Guo , Tang Qi-Zhu 
Published: June 2021

AbstractProteasomal activity is compromised in diabetic hearts that contributes to proteotoxic stresses and cardiac dysfunction. Osteocrin (OSTN) acts as a novel exercise-responsive myokine and is implicated in various cardiac diseases. Herein, we aim to investigate the role and underlying molecular basis of OSTN in diabetic cardiomyopathy (DCM). Mice received a single intravenous injection of the cardiotrophic adeno-associated virus serotype 9 to overexpress OSTN in the heart and then were exposed to intraperitoneal injections of streptozotocin (STZ, 50 mg/kg) for consecutive 5 days to generate diabetic models. Neonatal rat cardiomyocytes were isolated and stimulated with high glucose to verify the role of OSTN in vitro. OSTN expression was reduced by protein kinase B/forkhead box O1 dephosphorylation in diabetic hearts, while its overexpression significantly attenuated cardiac injury and dysfunction in mice with STZ treatment. Besides, OSTN incubation prevented, whereas OSTN silence aggravated cardiomyocyte apoptosis and injury upon hyperglycemic stimulation in vitro. Mechanistically, OSTN treatment restored protein kinase G (PKG)-dependent proteasomal function, and PKG or proteasome inhibition abrogated the protective effects of OSTN in vivo and in vitro. Furthermore, OSTN replenishment was sufficient to prevent the progression of pre-established DCM and had synergistic cardioprotection with sildenafil. OSTN protects against DCM via restoring PKG-dependent proteasomal activity and it is a promising therapeutic target to treat DCM.
Structural basis for late maturation steps of the human mitoribosomal large subunit
Authors: Cipullo Miriam , Gesé Genís Valentín , Khawaja Anas , Hällberg B. Martin , Rorbach Joanna 
Published: June 2021

AbstractMitochondrial ribosomes (mitoribosomes) synthesize a critical set of proteins essential for oxidative phosphorylation. Therefore, mitoribosomal function is vital to the cellular energy supply. Mitoribosome biogenesis follows distinct molecular pathways that remain poorly understood. Here, we determine the cryo-EM structures of mitoribosomes isolated from human cell lines with either depleted or overexpressed mitoribosome assembly factor GTPBP5, allowing us to capture consecutive steps during mitoribosomal large subunit (mt-LSU) biogenesis. Our structures provide essential insights into the last steps of 16S rRNA folding, methylation and peptidyl transferase centre (PTC) completion, which require the coordinated action of nine assembly factors. We show that mammalian-specific MTERF4 contributes to the folding of 16S rRNA, allowing 16 S rRNA methylation by MRM2, while GTPBP5 and NSUN4 promote fine-tuning rRNA rearrangements leading to PTC formation. Moreover, our data reveal an unexpected involvement of the elongation factor mtEF-Tu in mt-LSU assembly, where mtEF-Tu interacts with GTPBP5, similar to its interaction with tRNA during translational elongation.
Limited survival and impaired hepatic fasting metabolism in mice with constitutive Rag GTPase signaling
Authors: de la Calle Arregui Celia , Plata-Gómez Ana Belén , Deleyto-Seldas Nerea , García Fernando , Ortega-Molina Ana , Abril-Garrido Julio , Rodriguez Elena , Nemazanyy Ivan , Tribouillard Laura , de Martino Alba , Caleiras Eduardo , Campos-Olivas Ramón , Mulero Francisca , Laplante Mathieu , Muñoz Javier , Pende Mario , Sabio Guadalupe , Sabatini David M. , Efeyan Alejo 
Published: June 2021

AbstractThe mechanistic target of rapamycin complex 1 (mTORC1) integrates cellular nutrient signaling and hormonal cues to control metabolism. We have previously shown that constitutive nutrient signaling to mTORC1 by means of genetic activation of RagA (expression of GTP-locked RagA, or RagAGTP) in mice resulted in a fatal energetic crisis at birth. Herein, we rescue neonatal lethality in RagAGTP mice and find morphometric and metabolic alterations that span glucose, lipid, ketone, bile acid and amino acid homeostasis in adults, and a median lifespan of nine months. Proteomic and metabolomic analyses of livers from RagAGTP mice reveal a failed metabolic adaptation to fasting due to a global impairment in PPARα transcriptional program. These metabolic defects are partially recapitulated by restricting activation of RagA to hepatocytes, and revert by pharmacological inhibition of mTORC1. Constitutive hepatic nutrient signaling does not cause hepatocellular damage and carcinomas, unlike genetic activation of growth factor signaling upstream of mTORC1. In summary, RagA signaling dictates dynamic responses to feeding-fasting cycles to tune metabolism so as to match the nutritional state.
Two-dimensional ferroelasticity in van der Waals β’-In2Se3
Authors: Xu Chao , Mao Jianfeng , Guo Xuyun , Yan Shanru , Chen Yancong , Lo Tsz Wing , Chen Changsheng , Lei Dangyuan , Luo Xin , Hao Jianhua , Zheng Changxi , Zhu Ye 
Published: June 2021

AbstractTwo-dimensional (2D) materials exhibit remarkable mechanical properties, enabling their applications as flexible and stretchable ultrathin devices. As the origin of several extraordinary mechanical behaviors, ferroelasticity has also been predicted theoretically in 2D materials, but so far lacks experimental validation and investigation. Here, we present the experimental demonstration of 2D ferroelasticity in both exfoliated and chemical-vapor-deposited β’-In2Se3 down to few-layer thickness. We identify quantitatively 2D spontaneous strain originating from in-plane antiferroelectric distortion, using both atomic-resolution electron microscopy and in situ X-ray diffraction. The symmetry-equivalent strain orientations give rise to three domain variants separated by 60° and 120° domain walls (DWs). Mechanical switching between these ferroelastic domains is achieved under ≤0.5% external strain, demonstrating the feasibility to tailor the antiferroelectric polar structure as well as DW patterns through mechanical stimuli. The detailed domain switching mechanism through both DW propagation and domain nucleation is unraveled, and the effects of 3D stacking on such 2D ferroelasticity are also discussed. The observed 2D ferroelasticity here should be widely available in 2D materials with anisotropic lattice distortion, including the 1T’ transition metal dichalcogenides with Peierls distortion and 2D ferroelectrics such as the SnTe family, rendering tantalizing potential to tune 2D functionalities through strain or DW engineering.
Theoretical demonstration of a capacitive rotor for generation of alternating current from mechanical motion
Authors: Haimov Ehud , Chapman Aidan , Bresme Fernando , Holmes Andrew S. , Reddyhoff Tom , Urbakh Michael , Kornyshev Alexei A. 
Published: June 2021

AbstractInnovative concepts and materials are enabling energy harvesters for slower motion, particularly for personal wearables or portable small-scale applications, hence contributing to a future sustainable economy. Here we propose a principle for a capacitive rotor device and analyze its operation. This device is based on a rotor containing many capacitors in parallel. The rotation of the rotor causes periodic capacitance changes and, when connected to a reservoir-of-charge capacitor, induces alternating current. The properties of this device depend on the lubricating liquid situated between the capacitor’s electrodes, be it a highly polar liquid, organic electrolyte, or ionic liquid – we consider all these scenarios. An advantage of the capacitive rotor is its scalability. Such a lightweight device, weighing tens of grams, can be implemented in a shoe sole, generating a significant power output of the order of Watts. Scaled up, such systems can be used in portable wind or water turbines.
Timing of iceberg scours and massive ice-rafting events in the subtropical North Atlantic
Authors: Condron Alan , Hill Jenna C. 
Published: June 2021

AbstractHigh resolution seafloor mapping shows extraordinary evidence that massive (>300 m thick) icebergs once drifted >5,000 km south along the eastern United States, with >700 iceberg scours now identified south of Cape Hatteras. Here we report on sediment cores collected from several buried scours that show multiple plow marks align with Heinrich Event 3 (H3), ~31,000 years ago. Numerical glacial iceberg simulations indicate that the transport of icebergs to these sites occurs during massive, but short-lived, periods of elevated meltwater discharge. Transport of icebergs to the subtropics, away from deep water formation sites, may explain why H3 was associated with only a modest increase in ice-rafting across the subpolar North Atlantic, and implies a complex relationship between freshwater forcing and climate change. Stratigraphy from subbottom data across the scour marks shows there are additional features that are both older and younger, and may align with other periods of elevated meltwater discharge.
Ceramide structure dictates glycosphingolipid nanodomain assembly and function
Authors: Arumugam Senthil , Schmieder Stefanie , Pezeshkian Weria , Becken Ulrike , Wunder Christian , Chinnapen Dan , Ipsen John Hjort , Kenworthy Anne K. , Lencer Wayne , Mayor Satyajit , Johannes Ludger 
Published: June 2021

AbstractGangliosides in the outer leaflet of the plasma membrane of eukaryotic cells are essential for many cellular functions and pathogenic interactions. How gangliosides are dynamically organized and how they respond to ligand binding is poorly understood. Using fluorescence anisotropy imaging of synthetic, fluorescently labeled GM1 gangliosides incorporated into the plasma membrane of living cells, we found that GM1 with a fully saturated C16:0 acyl chain, but not with unsaturated C16:1 acyl chain, is actively clustered into nanodomains, which depends on membrane cholesterol, phosphatidylserine and actin. The binding of cholera toxin B-subunit (CTxB) leads to enlarged membrane domains for both C16:0 and C16:1, owing to binding of multiple GM1 under a toxin, and clustering of CTxB. The structure of the ceramide acyl chain still affects these domains, as co-clustering with the glycosylphosphatidylinositol (GPI)-anchored protein CD59 occurs only when GM1 contains the fully saturated C16:0 acyl chain, and not C16:1. Thus, different ceramide species of GM1 gangliosides dictate their assembly into nanodomains and affect nanodomain structure and function, which likely underlies many endogenous cellular processes.
Future climate change shaped by inter-model differences in Atlantic meridional overturning circulation response
Authors: Bellomo Katinka , Angeloni Michela , Corti Susanna , von Hardenberg Jost 
Published: June 2021

AbstractIn climate model simulations of future climate change, the Atlantic Meridional Overturning Circulation (AMOC) is projected to decline. However, the impacts of this decline, relative to other changes, remain to be identified. Here we address this problem by analyzing 30 idealized abrupt-4xCO2climate model simulations. We find that in models with larger AMOC decline, there is a minimum warming in the North Atlantic, a southward displacement of the Inter-tropical Convergence Zone, and a poleward shift of the mid-latitude jet. The changes in the models with smaller AMOC decline are drastically different: there is a relatively larger warming in the North Atlantic, the precipitation response exhibits a wet-get-wetter, dry-get-drier pattern, and there are smaller displacements of the mid-latitude jet. Our study indicates that the AMOC is a major source of inter-model uncertainty, and continued observational efforts are needed to constrain the AMOC response in future climate change.
Symmetry-enforced topological nodal planes at the Fermi surface of a chiral magnet
Authors: Wilde Marc A. , Dodenhöft Matthias , Niedermayr Arthur , Bauer Andreas , Hirschmann Moritz M. , Alpin Kirill , Schnyder Andreas P. , Pfleiderer Christian 
Published: June 2021

AbstractDespite recent efforts to advance spintronics devices and quantum information technology using materials with non-trivial topological properties, three key challenges are still unresolved1–9. First, the identification of topological band degeneracies that are generically rather than accidentally located at the Fermi level. Second, the ability to easily control such topological degeneracies. And third, the identification of generic topological degeneracies in large, multisheeted Fermi surfaces. By combining de Haas–van Alphen spectroscopy with density functional theory and band-topology calculations, here we show that the non-symmorphic symmetries10–17 in chiral, ferromagnetic manganese silicide (MnSi) generate nodal planes (NPs)11,12, which enforce topological protectorates (TPs) with substantial Berry curvatures at the intersection of the NPs with the Fermi surface (FS) regardless of the complexity of the FS. We predict that these TPs will be accompanied by sizeable Fermi arcs subject to the direction of the magnetization. Deriving the symmetry conditions underlying topological NPs, we show that the 1,651 magnetic space groups comprise 7 grey groups and 26 black-and-white groups with topological NPs, including the space group of ferromagnetic MnSi. Thus, the identification of symmetry-enforced TPs, which can be controlled with a magnetic field, on the FS of MnSi suggests the existence of similar properties—amenable for technological exploitation—in a large number of materials.