bluuebunny/crossref_metadata_2025_split huggingface dataset:
Marinicaulis
Authors: Sun Cong 
Published: February 2021

Abstract

Ma.ri.ni.cau'lis. L. masc. adj.
marinus
of the sea; L. masc. n.
caulis
stalk; N.L. masc. n.
Marinicaulis
a stalk from the sea.

Proteobacteria / Alphaproteobacteria / Parvularculales / Parvularculaceae / Marinicaulis

The genus
Marinicaulis
accommodates mesophilic, neutrophilic, halotolerant, and chemoorganotrophic bacteria. Cells are Gram‐stain‐negative, aerobic or facultatively anaerobic, ovoid or short rod‐shaped with prosthecae or flagella, non‐spore‐forming, and slightly motile with flagella. Growth occurs at pH 5.0–9.0, 4–40°C, and a wide range of NaCl concentrations (0.5–16%). No growth is observed without NaCl. Catalase‐positive or ‐negative and oxidase‐positive. The major isoprenoid quinone is Q‐10. The principal cellular fatty acids (>10%) are: C
16:0
, 11‐methyl C
18:1
ω7
c
, and summed feature 3 (C
16:1
ω7
c
and/or C
16:1
ω6
c
). Summed feature 8 (C
18:1
ω7
c
and/or C
18:1
ω6
c
) presents as major fatty acid in some species. The major polar lipids include phosphatidylglycerol (PG) or phosphatidylethanolamine (PE) and unidentified glycolipids.
Marinicaulis
species can utilize monosaccharides, oligosaccharides, sugar alcohol, amino acids, and organic acids. Genome features show that
Marinicaulis flavus
SY‐3‐19
T
has putative β‐etherase‐encoding sequences, which is the first report of β‐etherases in the order “
Parvularculales
”, indicating the potential ability to degrade lignin model substrates. The known habitat is seawater.

DNA G + C content (mol%)
:
58.2–60.9 (genome sequence).

Type species
:
Marinicaulis flavus
Yu et al. 2018
VP
.
Mangrovitalea
Authors: Tian Yun , Liao Hu 
Published: February 2021

Abstract

Man.gro.vi.ta'le.a N.L. neut. n.
mangrovum
mangrove; N.L. fem. n.
talea
a rod; N.L. fem. n.
Mangrovitalea
.

Proteobacteria / Gammaproteobacteria / Alteromonadales / Alteromonadaceae / Mangrovitalea

The genus
Mangrovitalea
accommodates mildly acidophilic, facultatively anaerobic, and oligotrophic bacteria. Cells are Gram‐stain‐negative, rod shaped, and motile with a single polar flagellum. Growth is observed at temperatures from 10 to 40°C (optimum 30°C), salinities from 0.5 to 6% (optimum 2–3%), and pH from 5 to 8 (optimum 6). The G + C content of the genomic DNA is 58.2%. The dominant fatty acids (>5%) are C
16:0
(32.3%), C
16:0
N‐alcohol (14.8%), summed feature 9 (comprising iso‐C
17:1
ω9
c
and/or C
16:0
10‐methyl, 13.3%), and summed feature 3 (comprising C
16:1
ω7
c
and/or C
16:1
ω6
c
, 8.2%). The predominant respiratory quinone is ubiquinone‐9, and the major polar lipids are diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, and an unidentified aminophospholipid. The known habitat is mangrove sediments.

DNA G + C content (mol%)
:
58.2.

Type species
:
Mangrovitalea sediminis
Liao et al. 2017
VP
.
Zavarzinella
Authors: Dedysh Svetlana N. , Kulichevskaya Irina S. 
Published: February 2021

Abstract

Za.var.zi.nel'la. N.L. fem. dim. n.
Zavarzinella
, named in honor of the famous Russian microbiologist George A. Zavarzin, for his outstanding contribution to increasing our knowledge of microbial diversity.

Planctomycetes / Planctomycetia / Gemmatales / Gemmataceae / Zavarzinella

The genus
Zavarzinella
is represented by planctomycetes with ellipsoid cells, which occur singly or are assembled in large rosettelike clusters. Cell division occurs by means of budding. Daughter cells are motile, while adult cells are nonmotile. Cells produce thick stalks and attach to surfaces by means of amorphous holdfast material. Members of the genus are moderately acidophilic and mesophilic chemoorganotrophic aerobes. Most sugars and some polysaccharides are used as growth substrates. The major fatty acids are C
18:0
, C
18:1
ω5
c
, and C
16:1
ω5
c
. The major quinone is MK‐6. This genus is a member of the phylum
Planctomycetes
, class
Planctomycetia,
order
Gemmatales
, and family
Gemmataceae
. Representatives of this genus colonize wetlands and various freshwater habitats.

DNA G
+
C content
(
mol
%): 59.1 (genome sequence).

Type species
:
Zavarzinella formosa
Kulichevskaya et al. 2009
VP
.
Halopeptonella
Authors: Menes Rodolfo Javier 
Published: February 2021

Abstract

Ha.lo.pep.to.nel'la. Gr. masc. n.
hals
,
halos
salt; N.L. neut. n.
peptonum
, peptone; L. fem. dim. ending ‐
ella
; N.L. fem. n.
Halopeptonella
, a halophilic bacterium that grows solely with peptone.

Proteobacteria / Gammaproteobacteria / Chromatiales / Ectothiorhodospiraceae / Halopeptonella

The genus
Halopeptonella
, classified within the family
Ectothiorhodospiraceae
, and within the class
Gammaproteobacteria
, currently consists of a single species,
Halopeptonella vilamensis
. It was isolated in pure culture from the sediment of a hypersaline Andean lake located at 4,600 m above sea level (Laguna Vilama, Argentina). The cells are Gram‐stain‐negative, nonmotile, non‐spore‐forming, heterotrophic, and strictly aerobic rods. Colonies are pink, circular, smooth, convex, and pinpoint. Cells are halophilic, capable of growing at NaCl concentrations from 7.5 to 25% (w/v). Cells grow in media containing tryptone or peptone as sole carbon and energy source. Require yeast extract, sodium, and magnesium but not potassium ions for growth.

DNA G + C content (mol%)
: 64.2 (genome).

Type species
:
Halopeptonella vilamensis
Menes et al. 2016, VL168.
Clinical Experience With a Multidisciplinary Model of Vascular Ultrasound for the Evaluation for Giant Cell Arteritis
Authors: Tedeschi Sara K. , Sobiesczyzk Piotr S. , Ford Julia A. , DiIorio Michael A. , Docken William P. 
Published: February 2021

ObjectiveVascular ultrasound (VUS) is a first‐line test for giant cell arteritis (GCA) in Europe but has been of limited use in the United States. We report clinical experience with a multidisciplinary model of VUS for the evaluation of GCA at a large US medical center.MethodsPatients who underwent VUS for evaluation of GCA between 2013 and 2017 were reviewed. Trained vascular technologists followed a standardized protocol to visualize bilateral temporal, carotid, subclavian, and axillary arteries. Vascular medicine physicians interpreted VUS as no arteritis, hyperechoic wall thickening, or acute arteritis. Characteristics of patients with versus without acute arteritis (no arteritis or hyperechoic wall thickening) were compared. Among patients with suspected new‐onset GCA, the treating physician’s pretest and posttest suspicion for GCA were compared.ResultsOf 530 patients, 10.6% had prior‐onset GCA, 31.7% had polymyalgia rheumatica, and 57.6% were taking glucocorticoids. Most patients had no arteritis on VUS (84.3%); 10.6% had acute arteritis, and 5.1% had hyperechoic wall thickening. Typical GCA symptoms, such as jaw claudication and scalp tenderness, were significantly more frequent in patients with acute arteritis. For all 42 patients with suspected new‐onset GCA and acute arteritis, posttest suspicion was unchanged or increased. Of 415 patients with suspected new‐onset GCA and VUS without acute arteritis, suspicion decreased (76.4%) or was unchanged (20.2%).ConclusionWe describe a multidisciplinary model for incorporating VUS into GCA care. When pretest suspicion was low and VUS did not reveal acute arteritis, posttest suspicion typically decreased, whereas when pretest suspicion was high and VUS revealed acute arteritis, posttest suspicion was reinforced.
The Role of Knowledge and Attitudes About Nonsteroidal Anti‐inflammatory Drugs in Determining Treatment Use
Authors: Vina E. R. , Hannon M. J. , Quinones C. , Hausmann L. R. M. , Ibrahim S. A. , Dagnino J. , Kwoh C. K. 
Published: February 2021

ObjectiveThe objective of this study was to evaluate how patient knowledge and beliefs regarding nonsteroidal anti‐inflammatory drugs (NSAIDs) may influence the use of NSAIDs for osteoarthritis (OA).MethodsSurveys of 334 adults with knee and/or hip OA were analyzed in this cross‐sectional study. Familiarity with and perceptions of benefits/risks of NSAID use were measured to assess associations with the use of prescription and nonprescription oral NSAIDs. Multinomial logistic regression models were adjusted for sociodemographic and clinical variables.ResultsIn this sample, 35.9% and 35.6% reported use of oral prescription and nonprescription‐only NSAIDs, respectively. Hispanic participants, compared with non‐Hispanic White participants, had lower perceived benefit (P = 0.005) and risk (P = 0.001) of prescription NSAIDs. The following were associated with prescription NSAID use instead of no NSAID use: having family/friends who used prescription (relative risk ratio [RRR] 3.91; 95% confidence interval [CI] 2.05‐7.47) and over‐the‐counter (OTC) (RRR 3.10; 95% CI 1.65‐5.83) NSAIDs for OA, understanding the consequences of using both prescription (RRR 3.50; 95% CI 1.79‐6.86) and OTC (RRR 2.80; 95% CI 1.39‐5.65) NSAIDs, higher perceived benefit of both prescription (RRR 2.51; 95% CI 1.71‐3.66) and OTC (RRR 1.44; 95% CI 1.01‐2.06) NSAIDs, and lower perceived risk of both types of NSAIDs (prescription: RRR 0.63 [95% CI 0.46‐0.87]; OTC: RRR 0.53 [95% CI 0.37‐0.75]). Similar results were found when we assessed the relationship between these variables and OTC NSAID use versus no oral NSAID use.ConclusionAdults with knee and/or hip OA were more likely to use NSAIDs if they were more familiar with, had an increased perceived benefit of, and had a decreased perceived risk of these drugs. Patients’ perceptions and beliefs about NSAIDs should be evaluated when considering them for treatment.
Efficient Human Cell Coexpression System and Its Application to the Production of Multiple Coronavirus Antigens
Authors: Kim Chonsaeng , Jeong You Kyeong , Yu Jihyeon , Shin Hye Jin , Ku Keun Bon , Cha Hyung Jin , Han Jun Hee , Hong Sung‐Ah , Kim Bum‐Tae , Kim Seong‐Jun , Woo Jae‐Sung , Bae Sangsu 
Published: February 2021

AbstractCoproduction of multiple proteins at high levels in a single human cell line would be extremely useful for basic research and medical applications. Here, a novel strategy for the stable expression of multiple proteins by integrating the genes into defined transcriptional hotspots in the human genome is presented. As a proof‐of‐concept, it is shown that EYFP is expressed at similar levels from hotspots and that the EYFP expression increases proportionally with the copy number. It is confirmed that three different fluorescent proteins, encoded by genes integrated at different loci, can be coexpressed at high levels. Further, a stable cell line is generated, producing antigens from different human coronaviruses: MERS‐CoV and HCoV‐OC43. Antibodies raised against these antigens, which contain human N‐glycosylation, show neutralizing activities against both viruses, suggesting that the coexpression system provides a quick and predictable way to produce multiple coronavirus antigens, such as the recent 2019 novel human coronavirus.
Blue Light‐Operated CRISPR/Cas13b‐Mediated mRNA Knockdown (Lockdown)
Authors: Blomeier Tim , Fischbach Patrick , Koch Leonie‐Alexa , Andres Jennifer , Miñambres Miguel , Beyer Hannes Michael , Zurbriggen Matias Daniel 
Published: February 2021

AbstractThe introduction of optogenetics into cell biology has furnished systems to control gene expression at the transcriptional and protein stability level, with a high degree of spatial, temporal, and dynamic light‐regulation capabilities. Strategies to downregulate RNA currently rely on RNA interference and CRISPR/Cas‐related methods. However, these approaches lack the key characteristics and advantages provided by optical control. “Lockdown” introduces optical control of RNA levels utilizing a blue light‐dependent switch to induce expression of CRISPR/Cas13b, which mediates sequence‐specific mRNA knockdown. Combining Lockdown with optogenetic tools to repress gene‐expression and induce protein destabilization with blue light yields efficient triple‐controlled downregulation of target proteins. Implementing Lockdown to degrade endogenous mRNA levels of the cyclin‐dependent kinase 1 (hCdk1) leads to blue light‐induced G2/M cell cycle arrest and inhibition of cell growth in mammalian cells.
Domain‐Size‐Dependent Residual Stress Governs the Phase‐Transition and Photoluminescence Behavior of Methylammonium Lead Iodide
Authors: Lee Kwang Jae , Turedi Bekir , Giugni Andrea , Lintangpradipto Muhammad Naufal , Zhumekenov Ayan A. , Alsalloum Abdullah Y. , Min Jung‐Hong , Dursun Ibrahim , Naphade Rounak , Mitra Somak , Roqan Iman S. , Ooi Boon S. , Mohammed Omar F. , Fabrizio Enzo Di , Cho Namchul , Bakr Osman M. 
Published: February 2021

AbstractMethylammonium lead iodide (MAPbI3) perovskite has garnered significant interest as a versatile material for optoelectronic applications. The temperature‐dependent photoluminescence (TDPL) and phase‐transition behaviors revealed in previous studies have become standard indicators of defects, stability, charge carrier dynamics, and device performance. However, published reports abound with examples of irregular photoluminescence and phase‐transition phenomena that are difficult to reconcile, posing major challenges in the correlation of those properties with the actual material state or with the subsequent device performance. In this paper, a unifying explanation for the seemingly inconsistent TDPL and phase‐transition (orthorhombic‐to‐tetragonal) characteristics observed for MAPbI3 is presented. By investigating MAPbI3 perovskites with varying crystalline states, ranging from polycrystal to highly oriented crystal as well as single‐crystals, key features in the TDPL and phase‐transition behaviors are identified that are related to the extent of crystal domain‐size‐dependent residual stress and stem from the considerable volume difference (ΔV ≈ 4.5%) between the primitive unit cells of the orthorhombic (at 80 K) and tetragonal phases (at 300 K) of MAPbI3. This fundamental connection is essential for understanding the photophysics and material processing of soft perovskites.
Dendrite‐Free Li‐Metal Anode Enabled by Dendritic Structure
Authors: Zhang Hongyu , Ju Shunlong , Xia Guanglin , Sun Dalin , Yu Xuebin 
Published: February 2021

AbstractThe practical application of Li‐metal anode in high‐energy rechargeable Li batteries is still hindered by the uncontrollable formation of Li dendrites. Here, a facile way is reported to stabilize Li‐metal anode by building dendrite‐like Li3Mg7 alloys enriched with Li‐containing polymers as the physical protecting layer and LiH as the Li‐ion conductor. This unique dendritic structure effectively reduces local current density and accommodates volume change during the repeated Li plating/stripping process. More importantly, lithiophilic Li3Mg7 alloys not only guide the uniform Li deposition down into the below Li metal upon Li deposition, but also thermodynamically promote the extraction of Li during the reverse Li stripping process, which suppresses the parasitic reactions occurring on the surface of Li metal and hence inhibits the formation of Li dendrites. Moreover, the facile diffusion of Mg from Li3Mg7 alloys toward Li metal below is thermodynamically permitted, which leads to a uniform distribution of LiMg alloys inside the whole electrode and thus benefits long‐term deep cycling stability. As a result, the protected Li‐metal anode delivers stable and dendrite‐free cycling performance at 10 mA h cm−2 for over 900 h. When coupling this anode with LiFePO4 and S cathodes, the thus‐assembled full cells exhibit superior cycling stability.
Porphyrin Conjugated Polymer with Periodic Type II‐Like Heterojunctions and Single‐Atom Catalytic Sites for Broadband‐Responsive Hydrogen Evolution
Authors: Wang Jinming , Xu Lei , Wang Tingxia , Chen Shengtao , Jiang Zhuo , Li Renjie , Zhang Yuexing , Peng Tianyou 
Published: February 2021

AbstractA novel heterometallic Zn‐/Co‐porphyrin conjugated polymer (ZnCoP‐F CP) with its Co‐porphyrin bridging unit bearing two perfluorophenyls is synthesized via a Sonogashira coupling reaction. The resulting ZnCoP‐F CP without the Pt cocatalyst exhibits broadband (UV–vis–NIR) light‐driven hydrogen evolution activity of 83 µmol h−1, which is more than twice that (39 µmol h−1) of its counterpart (ZnCoP CP) with the Co‐porphyrin unit bearing two phenyls. Furthermore, an apparent quantum yield of 6.92%, 5.50%, 5.78%, 3.17%, and 0.73% is achieved from the ZnCoP‐F CP illuminated at 400, 500, 550, 700, and 850 nm monochromatic light, respectively. The enhanced performance of ZnCoP‐F CP can be attributed to the high electron‐withdrawing capacity of its perfluorophenyls and the highly dispersed Co centers in Co‐porphyrin bridging units serving as single‐atom catalytic sites (CoN4), which facilitate the formation of periodic type II‐like heterojunctions and the fast charge transfer from Zn‐porphyrin to Co‐porphyrin units for hydrogen evolution reaction. The broadband‐responsive feature is due to the large π‐conjugated system through the whole 2D internal framework of the porphyrin polymer. This work opens a new path to fabricate porphyrin‐based polymer with an efficient and broadband‐responsive hydrogen evolution system.
Liquid Crystal Soft Actuators and Robots toward Mixed Reality
Authors: Zhu Chongyu , Lu Yao , Jiang Lixin , Yu Yanlei 
Published: February 2021

AbstractLiquid crystals (LCs) are soft but smart materials that can adjust its chemical or physical properties in response to various external stimuli. Using these materials to construct soft actuators and robots, referred as LC actuators and robots, is expected to replace current machinery part, obtaining lighter and smaller equipment with adjustable and complex functions. Especially, combining these LC actuator and robots with existing virtual reality and augmented reality technologies will produce a new world of mixed reality (MR) with the visual, auditory, and somatosensory interaction. In this review, the recent work on responsive LC actuators and robots is introduced, emphasizing on their potentials in haptic use. By discussing their programmable control via suitable stimuli, the LC actuators and robots are summarized for mechanical outputs, environmental mimic, and fine‐tuning of surface texture and roughness. It is anticipated that the continuous development on LC actuators and robots will accelerate the MR technology toward practical application.
Nano‐Oncologicals: A Tortoise Trail Reaching New Avenues
Authors: Dacoba Tamara G. , Anthiya Shubaash , Berrecoso Germán , Fernández‐Mariño Iago , Fernández‐Varela Carmen , Crecente‐Campo José , Teijeiro‐Osorio Desirée , Torres Andón Fernando , Alonso María J. 
Published: February 2021

AbstractResearch efforts towards cancer therapeutics have resulted in the development of a variety of pharmacological molecules, including small synthetic molecules and biological drugs (RNA‐based therapies and monoclonal antibodies—mAbs), intended to target tumor or immune‐related cells, or their signaling mediators. The majority of them present important biopharmaceutical problems related to their difficulties for overcoming biological barriers and reach their targets. Nanotechnology has been, for more than 60 years, trying to solve these problems. As knowledge in drug discovery, molecular biology, and biomaterials advances, there has been significant progress in the adequate design of nanodelivery strategies that may significantly contribute to the exploitation of the new therapies. This review provides a critical overview of the current potential of nanotechnology to solve problems associated with the different categories of drugs. Starting with the general concept of passive and active targeting, it presents the distinct advantages that delivery technologies have shown to date for improving the therapeutic outcome of small drugs with cytotoxic activity, RNA‐ and mAb‐based therapies. Moreover, it precisely describes the benefits of combining immunotherapies and nanotechnology. The most advanced technologies are put into perspective in relation to their translational pathway and the future avenues for nano‐oncologicals.
Dye‐Sensitized Downconversion Nanoprobes with Emission Beyond 1500 nm for Ratiometric Visualization of Cancer Redox State
Authors: Wang Chenlu , Lin Hongxin , Ge Xiaoguang , Mu Jing , Su Lichao , Zhang Xuan , Niu Meng , Yang Huanghao , Song Jibin 
Published: February 2021

AbstractDetection of glutathione (GSH) in the body is essential to accurately map the redox state of cells and real‐time visualization of physiological and pathological conditions in vivo. However, traditional fluorescence (FL) imaging in the near‐infrared I region (NIR‐I, 650–900 nm) is difficult to quantitively visualize GSH in vivo due to the tissue autofluorescence background and disastrous photon scattering. Herein, a NIR‐IIb (1500–1700 nm) nanoprobe consisting of 4‐nitrophenol‐Cy7 (NPh) conjugated lanthanide‐based downconversion nanoparticles (DCNP@NPh‐PEG) is developed for in vivo ratiometric imaging of GSH. In the presence of GSH, NPh shows responsively enhanced FL emission at 808 nm, thus enhancing FL signal at 1550 nm of DCNPs excited by 808 nm (F1550, 808Ex) through non‐radiative energy transfer (NRET) effect, while the fluorescence of DCNP at 1550 nm excited by 980 nm laser (F1550, 980Ex) is stable because no NRET occurred. The ratiometric F1550, 980Ex/F1550, 808Ex value exhibits a linearship with GSH concentration ranged from 0–24 mm with detection limit of 0.3 mm. The NIR‐IIb nanoprobe has excellent performance in detecting and imaging GSH in both subcutaneous tumor and orthotopic colon tumor in vivo with high accuracy and resolution. The design strategy of the ratiometric NIR‐II FL nanoprobe based on the activated FERT effect provides a reliable tool for the development of NIR‐II nanoprobes for accurate biosensing in vivo.
Understanding the Ion Transport Behavior across Nanofluidic Membranes in Response to the Charge Variations
Authors: Hou Linxiao , Xian Weipeng , Bing Shaosuo , Song Yanpei , Sun Qi , Zhang Lin , Ma Shengqian 
Published: February 2021

AbstractBiological pores regulate the cellular traffic of a diverse collection of molecules, often with extremely high selectivity. Given the ubiquity of charge‐based separation in nature, understanding the link between the charged functionalities and the ion transport activities is essential for designing delicate separations, with the correlation being comparatively underdeveloped. Herein, the effect of charge density from the impact of pore structure is decoupled using a multivariate strategy for the construction of covalent organic framework‐based membranes. How the density of charged sites in the nanofluidic membranes affect the ion transport activity with particular emphasis on Li+ and Mg2+ ions, relevant to the challenge of salt‐lake lithium mining is systematically investigated. Systematic control of the charge distribution produces membranes with numerous advantages, overcoming the long‐term challenge of Li+/Mg2+ separation. The top membrane exhibits an outstanding equilibrium selectivity for Li+ over Mg2+ and operational stability under diffusion dialysis and electrodialysis conditions (Li+/Mg2+ up to 500), qualifying it as a potential candidate for lithium extraction. It is anticipated that the developed nanofluidic membrane platform can be further leveraged to tackle other challenges in controlled separation processes.