bluuebunny/crossref_metadata_2025_split huggingface dataset:
Differential Expression Spectrum of circRNA in Plasma Exosomes in Dilated Cardiomyopathy With Heart Failure
Authors: Xu Shuai , Zhang Ge , Tan Xin , Zeng Yiyao , Fan Huimin , Gao Jiamin , Qin Zhen , Yu Fengyi , Ma Bin , Zhang Ting , Jiang Hezi , Li Xian , Wang Xiangyu , Fan Jili , Bo Xiaohong , Zhou Yafeng , Tang Junnan 
Published: December 2024

ABSTRACTDilated cardiomyopathy (DCM), a form of non‐ischaemic myocardial disease, is characterised by structural and functional cardiac abnormalities. As defined by the World Health Organisation, DCM constitutes a significant cardiac pathology, leading to increased morbidity and mortality due to complications such as heart failure and arrhythmias. The diagnostic process for DCM predominantly employs echocardiography and MRI, with biomarkers like NT‐pro BNP and troponin providing supportive, yet non‐specific, evidence. Exosomes, small extracellular vesicles, play a critical role in intercellular communications by transferring biomolecules including lipids, proteins, messenger RNA (mRNA) and non‐coding RNA (ncRNA) to target cells, thereby influencing key cellular processes such as proliferation, differentiation, apoptosis, angiogenesis and immune modulation. Within the ncRNA category, circular RNAs (circRNAs) are notable for their cellular specificity and evolutionary conservation and are often implicated in the regulatory mechanisms underlying DCM and heart failure. This investigation employed next‐generation sequencing technology to analyse plasma exosomal circRNA profiles in DCM patients with chronic heart failure (CHF), compared to healthy controls. The analysis revealed distinct circRNA expression patterns, identifying 49 uniquely expressed circRNAs in the DCM cohort with CHF. These circRNAs were associated with several critical biological pathways, including the sequestration of extracellular ligands from receptors, N‐acetyltransferase activity, histone acetyltransferase activity and endocytic vesicle membrane composition. The findings of this study provide valuable insights into the pathophysiological mechanisms of DCM and offer evidence for improving clinical diagnostic methodologies.
CCL2/CCR2 Signalling in Mesenchymal Stem/Progenitor Cell Recruitment and Fracture Healing in Mice
Authors: Kannan Rahasudha , Koh Amy J. , Kent Robert N. , Bhutada Kaira , Wasi Fatima , Wagner Leon , Kozloff Kenneth , Baker Brendon M. , Roca Hernan , McCauley Laurie K. 
Published: December 2024

ABSTRACTMacrophage efferocytosis (clearance of apoptotic cells) is crucial for tissue homeostasis and wound repair, where macrophages secrete factors that promote resolution of inflammation and regenerative signalling. This study examined the role of efferocytic macrophage‐associated CCL2 secretion, its influence on mesenchymal stem/progenitor cell (MSPC) chemotaxis, and in vivo cell recruitment using Ccr2−/− (KO) mice with disrupted CCL2 receptor signalling in two regenerative models: ossicle implants and ulnar stress fractures. Single cell RNA sequencing and PCR validation indicated that efferocytosis of various apoptotic cells at bone injury sites (osteoblasts, pre‐osteoblasts, MSPC) upregulated CCL2. CCL2 gradients enhanced MSPC migration through type I collagen matrices. In vivo, MSPC (LepR+) infiltration was significantly reduced while macrophage (F4/80+) infiltration increased in KO ossicle implants versus WT. In ulnar stress fractures, micro‐CT revealed increased mineralized callus incidence in CCR2 KO male mice 5 days post injury (dpi) versus WT. By 7‐dpi callus fractional bone volume, trabecular thickness, and bone mineral density were increased versus WT. Immunohistochemistry of mice 5‐dpi confirmed an increase in callus area (including soft tissue); however, the percent of osteoprogenitors (%Osx+) within the callus was not different. These findings suggest that CCL2 differentially impacts MSPC recruitment depending on bone wound healing model.
Myeloperoxidase and Thyrotropin‐Releasing Hormone Within Leukaemia Stem Cells Increased Chemosensitivity in Acute Myeloid Leukaemia
Authors: Chen Chung‐Hsing , Chen Tsung‐Chih , Wu Ting‐Shuan , Hsiao Tzu‐Hung , Chen Jo‐Mei Maureen , Huang Chi‐Ying F. , Cheng Po‐Liang , Tsai Jia‐Rung , Teng Chieh‐Lin Jerry 
Published: December 2024

ABSTRACTLeukaemia stem cells (LSCs) are major contributors to chemoresistance in acute myeloid leukaemia (AML). Identifying potential biomarkers within LSCs that can predict chemosensitivity in AML is key. This prospective study involved 20 consecutive de novo AML patients who underwent ‘7 + 3’ induction therapy. The patients were divided into CR (n = 15) and non‐CR (n = 5) groups. Using single‐cell RNA sequencing, we examined the cellular states of bone marrow mononuclear cells from AML patients at diagnosis and identified LSC among these cells. Our results showed that in non‐CR AML patients, a significant increase in the proportion of immature cells during haematopoiesis within the AML cell populations was observed. Moreover, the expression of myeloperoxidase (MPO) (log2 fold‐change = 0.89; adjusted p < 0.0001) and thyrotropin‐releasing hormone (TRH) (log2 fold‐change = 0.65; adjusted p < 0.0001) was higher within LSCs in the CR group than in the non‐CR group. Furthermore, patients with higher expression of MPO and TRH demonstrated improved relapse‐free survival (p = 0.002 for MPO; p = 0.009 for TRH) and overall survival (p = 0.002 for MPO; p < 0.001 for TRH). The connection between MPO or TRH and chemosensitivity could be linked with the downregulation of transforming growth factor and the upregulation of interferon‐α. In conclusion, MPO and TRH in LSCs could serve as chemosensitivity biomarkers in AML.
Relation of 91 Circulating Inflammatory Proteins to Nonalcoholic Fatty Liver Disease: A Two‐Sample Mendelian Randomisation Study
Authors: Wu Xiaodong , Song Yanhong , Wu Shuodong 
Published: December 2024

ABSTRACTIncreasingly, emerging research evidence has demonstrated that nonalcoholic fatty liver disease (NAFLD) is a disease closely associated with systemic inflammation. However, the specific upstream inflammatory factors engaged in the pathogenesis of NAFLD remain unclear. Our study aimed to identify the inflammatory regulators causally associated with NAFLD pathogenesis through Mendelian randomisation. A two‐sample Mendelian randomisation method was applied to analyse the causal association between 91 circulating inflammatory proteins and NAFLD. Data on circulating inflammatory proteins were derived from samples of European ancestry (14,824 samples) and NAFLD data were obtained from the FinnGen consortium (2025 cases and 284,826 controls). Instrumental variables were selected from the genetic variance and F‐statistics were calculated to avoid bias. We adopted the random‐effects inverse variance weighting (IVW) method as our primary analytical approach. Supplementary analyses were also implemented, including weighted median, MR‐Egger and weighted mode. Moreover, we conducted pleiotropy and heterogeneity analyses to validate the accuracy of the findings. The application of Mendelian randomisation analysis identified four inflammatory factors that might be causally associated with NAFLD at the genetic level. Elevated levels of eotaxin (or = 1.27, 95% CI: 1.05–1.53, p = 0.014), osteoprotegerin (OPG) (or = 1.29, 1.03–1.60, p = 0.023) and TNFRSF9 (or = 1.32, 95% CI: 1.06–1.64, p = 0.014) may be causally related to an increasing risk of NAFLD. Conversely, heightened leukaemia inhibitory factor (LIF) levels (or = 0.63, 0.44–0.92, p = 0.016) were linked to a lower risk of NAFLD onset. There was no causal relationship between levels of other circulating inflammatory proteins and NAFLD. Our analysis uncovered four upstream inflammatory factors genetically associated with the pathogenesis of NAFLD. These results highlight the potential involvement of inflammation in NAFLD, which provides partial insights for further research in this field in the future.
Efficacy of Therapeutic Hypothermia During Transport of Newborns With Perinatal Hypoxic‐Ischaemic Encephalopathy: Experience From Newborn and Paediatric Transport Service, New South Wales
Authors: McLean Lucia , Dalrymple Hannah , Sasidharan Lekshmi , Grant Trish , Browning Carmo Kathryn 
Published: December 2024

ABSTRACTAimTo examine the efficacy of current non‐servo‐based cooling methods used by NETS NSW in treating hypoxic ischaemic encephalopathy (HIE) with therapeutic hypothermia (TH) in neonatal retrieval.MethodsA retrospective observational study of infants treated with TH for HIE retrieved by NETS NSW from January 2017 to June 2020 inclusive. Primary outcomes were the proportion of neonates achieving TH within 6 h of life and maintaining temperature in a therapeutic range.Results82 patients were included in analysis. Mean gestational age (GA) was 39 weeks (IQR 38–40) and mean birthweight (BW) 3297 g (SD 607 g). 68 infants (82.9%) were passively cooled while 14 (17.1%) were cooled with gel packs. 20 infants (21.4%) had rectal temperature monitoring before retrieval. 45 infants (55.6%) reached TH within 6 h of birth. Only 56 infants (68.3%) reached TH during retrieval. Of these infants, 45 (80.4%) had subsequent temperatures outside the therapeutic range.ConclusionsInfants receiving TH through non‐servo‐controlled methods are commonly exposed to temperature instability and overcooling. Introduction of servo‐controlled cooling devices is required to improve care and management of perinatal HIE in retrieval.
Biocontrol Potential of Bacillus velezensisZF‐10 Inhibiting Potato Virus Y and Promoting Growth of Tobacco Plant
Authors: Ying Yufeng , Zhang Nuoni , Li Bin , Liu Dongyang , Zhang Qiang , Yang Zhao , An Derong , Hao Xingan 
Published: December 2024

ABSTRACTThe viral disease caused by potato virus Y (PVY) poses a serious threat to tobacco plants worldwide. In this study, Bacillus velezensis ZF‐10, isolated from Panzhihua, China, exhibited an effective inhibitory effect against PVY. The presence of B. velezensis ZF‐10 significantly reduced the accumulation of PVY to 25.4% in plants and lowered the disease index to 15.23. Moreover, the infiltration of B. velezensis ZF‐10 on leaves of tobacco resulted in an increase in reactive oxygen species (ROS) and enhanced defence enzyme activity, leading to a decrease in malondialdehyde (MDA) accumulation and ultimately improving the tolerance of tobacco plants to PVY infection. Additionally, in a greenhouse environment, B. velezensis ZF‐10 promoted the growth of tobacco plants. Furthermore, B. velezensis ZF‐10 was found to exhibit activities such as ammonia production, cellulase, protease and siderophore production, and the ability to solubilise insoluble phosphates in soil. As a novel plant growth‐promoting bacterium (PGPB), B. velezensis ZF‐10 not only effectively suppressed the occurrence of PVY disease and enhanced tobacco growth but also demonstrated potent antifungal properties against various phytopathogens that cause diseases in tobacco plants and other crops. In conclusion, B. velezensis ZF‐10 is a potential biocontrol agent against PVY infection a supporter of plant growth for tobacco plant.
Interventions Intended to Improve the Well‐Being at Work of Nurses Working in Care Settings for Older People—A Systematic Review
Authors: Wiisak Johanna , Suikkala Arja , Leino‐Kilpi Helena , Stolt Minna , Suhonen Riitta , Koskinen Sanna 
Published: December 2024

ABSTRACTIntroductionNurses' well‐being at work (WAW) is important for overall health care outcomes. Nurses often navigate complex roles, contending with time constraints, ethical challenges and societal undervaluation, underscoring the necessity of addressing their WAW.MethodsThe aim of this systematic review was to analyse the interventions that potentially improve nurses' WAW in care settings for older people. The ultimate goal is to provide an understanding of this field and advance the development of WAW interventions. We performed a systematic review which was registered in PROSPERO and conducted according to PRISMA guideline. We conducted a comprehensive literature search across five scientific databases and one platform in February 2023.ResultsOut of 5975 records, we included 21 full‐text articles in the review. Interventions were typically complex and focused on (a) nurses' health, (b) nursing care, (c) care facilities and (d) management. Interventions resulted in a range of outcomes on the (a) physical, (b) psychosocial and (c) environmental dimensions of WAW, with most interventions leading to positive outcomes, albeit with instances of negative and neutral results.ConclusionsInterventions focusing on nurses' health or care facilities can be promising to improve WAW of nurses working in care settings for older people. Interventions aimed at improving the WAW have focused on various aspects. Despite the mainly positive outcomes, some interventions can also compromise nurses' WAW.Implications for PracticeStrategies and interventions aimed at improving nurses’ WAW are needed in practice as nurses’ WAW is crucial in recruiting to and retaining nurses in care settings for older people. Promoting WAW also contributes to the quality of care for older people and the provision of ethically high‐quality health services.Trial RegistrationThe review protocol was registered in the International Prospective Register of Systematic Reviews, PROSPERO (CRD42023399478)
Tolerance to insect herbivory increases with progressing plant development
Authors: Gols R. 
Published: December 2024

Abstract

Plants can sustain various degrees of damage or compensate for tissue loss by regrowth without significant fitness costs. This tolerance to insect herbivory depends on the plant's developmental stage during which the damage is inflicted and on how much tissue is removed.

Plant fitness correlates, that is, biomass and germination of seeds, were determined at different ontogenetic stages, vegetative, budding, or flowering stages of three annual brassicaceous species exposed to feeding by Pieris brassicae caterpillars at different intensities.

Fitness costs decreased with progressive ontogenetic stage at which damage was inflicted. Feeding on meristem tissues on vegetative and budding plants limited the plant's ability to fully compensate for tissue loss, whereas feeding on flowers resulted in full compensation or overcompensation in Sinapis arvensis and Brassica nigra. Herbivory promoted germination of seeds in the following year, thereby causing a shift in relative contribution to the next year's generation at the expense of contributing to the long‐lived seed bank. Herbivory intensity affected fitness correlates of B. nigra and to a lesser extent of Sisymbrium officinale, but not of S. arvensis, demonstrating that even closely related plant species can differ in their specific responses to herbivory and that these can differently affect reproductive output.

In terms of fitness costs, annual plant species can be quite resilient to herbivory. However, the extent to which they tolerate tissue loss depends on the ontogenetic stage that is under attack. Seed persistence in the soil has been proposed as a bet‐hedging strategy of short‐lived species to increase long‐term fitness. Herbivore‐induced changes in seed germination can result in a shift in the relative contribution of seeds to the seed bank and next year's generation.
Recognition of Novel KIR2DL1*0040135, KIR2DL1*112, KIR2DL1*0040136, KIR3DL1*0010122 Alleles From India
Authors: Agarwal Disha , Sharma Gaurav , Khadwal Alka , Malhotra Pankaj 
Published: December 2024

ABSTRACTNovel KIR alleles KIR2DL1*0040135, KIR2DL1*112, KIR2DL1*0040136 and KIR3DL1*0010122, were identified using next‐generation sequencing.
Kaempferol promotes flap survival by inhibiting ferroptosis and inflammation through network pharmacology and in vivo experiments
Authors: Wang An , Yang Jialong , Deng Jiapeng , Wang Kaitao , Chen Guodong , Lin Dingsheng 
Published: December 2024

AbstractSkin flap transplantation is a primary method for wound repair; however, postoperative skin flap necrosis remains a significant challenge. Kaempferol, a flavonol abundant in various foods, exhibits diverse pharmacological effects. This study investigated the potential targets of kaempferol for treating skin flap ischemia–reperfusion (I/R) injury through network pharmacology and molecular docking, followed by in vivo validation. Using SwissTargetPredict, PubChem, SymMap V2, and GeneCards databases, 174 potential target proteins of kaempferol were identified. KEGG and GO enrichment analyses, performed using R software, indicated that kaempferol promotes skin flap survival by modulating ferroptosis, TNF‐α, and NF‐κB signalling pathways. Molecular docking demonstrated stable binding between kaempferol and key proteins, including SIRT1 and NRF2. In vivo, a McFarlane skin flap model was established in Sprague–Dawley rats. Kaempferol treatment improved flap survival, enhanced perfusion areas and distal arteriole visualisation, and increased blood flow in the flap. Furthermore, kaempferol reduced neutrophil infiltration, alleviated oxidative stress, improved mitochondrial morphology and function, and inhibited the release of proinflammatory cytokines. Western blot and immunofluorescence analyses confirmed that kaempferol inhibited ferroptosis and inflammation while promoting flap survival. Mechanistically, kaempferol was found to activate SIRT1‐mediated HMGB1/TLR4/NF‐κB and NRF2/SLC7A11/GPX4 pathways, thereby promoting skin flap survival and mitigating I/R injury.
Spatial associations between measures of public transportation and diabetic foot ulcer outcomes in the state of Georgia: 2016–2019
Authors: Vanasse Lauren T , Chang Howard H , D'Souza Rohan , Ali Mohammed K , Waller Lance , Schechter Marcos C 
Published: December 2024

IntroductionThere are limited data regarding the associations between public transportation reliance, availability, and diabetic foot ulcer (DFU)-related amputations.Research design and methodsWe used visit-level data from the Georgia 2016–2019 Healthcare Cost and Utilization Project database and obtained transportation variables from open sources. Using Bayesian spatial-temporal models, we assessed the associations between transportation and DFU-related amputations within each quartile of poverty status indicators at the ZIP code tabulation area (ZCTA) level. We used the proportion of adults who use public transportation to commute, distance to nearest transit stop, and per capita expense on public transportation as proxies for public transportation reliance, availability, and both, respectively.ResultsOf 114 606 DFUs, 21 388 (19%) were associated with a major or minor amputation. Among ZCTAs at the highest income quartile, reduced amputation risk was associated with the proportion of adults who use public transportation to commute to work (relative risk (RR)=0.29, 95% CI 0.09 to 0.97 per IQR increase of 1.13%) and per capita expense on public transportation (RR=0.78, 95% CI 0.63 to 0.78 per IQR increase of 6 cents). In metropolitan Georgia, a 1 IQR (261 m) increase in distance to the nearest transit stop was associated with lower amputation risk among ZCTAs at the lowest income quartile (RR=0.47, 95% CI 0.26 to 0.85).ConclusionIn Georgia, public transportation reliance and availability are protective against DFU-related amputations in high-income but not among low-income ZCTAs. Reducing disparities in DFU-related amputations requires interventions to mitigate transportation barriers to care.
Differential impact of lifestyle factors on 2-hour glucose values in individuals with type 2 diabetes: potential for more personalized interventions
Authors: Snel Tim , Krone Tanja , Kamstra Regina J M , Eggink Hannah M , Pijl Hanno , de Graaf Albert A , de Hoogh Iris M 
Published: December 2024

IntroductionLifestyle determinants of 2-hour glucose concentration in people with type 2 diabetes and interindividual differences need to be identified.Research design and methods38 participants with type 2 diabetes, treated with lifestyle advice and/or metformin, tracked their physical activity, sleep and dietary intake, while continuously monitoring interstitial glucose concentrations for 11 periods of four consecutive days each. A linear mixed-effects model was used to quantify the effect of sleep, stress, current glucose, carbohydrate intake and exercise on glucose levels 2 hours later.ResultsThe final model identified carbohydrate intake (grams) in the past 5 min as well as in the past 30 min, sleep duration during the previous night (hours) and physical activity (metabolic equivalents) over the past 12 hours as significant fixed effects that influenced glucose concentrations 2 hours later. In addition, carbohydrate intake in the past 5 and past 30 min, and physical activity in the past and future 30 min were included as random or individualized effects. Although carbohydrate intake led to increased glucose concentrations in 2 hours in all individuals, the magnitude of this effect varied between individuals. The physical activity on glucose concentrations in 2 hours varied among individuals as well, in terms of magnitude and in terms of direction (showing either increase or decline).ConclusionsCarbohydrate intake, sleep and physical activity at specific points in time have both fixed as well as individualized effects on glucose concentrations 2 hours later in individuals with type 2 diabetes. Interindividual differences in glycemic response to lifestyle components call for personalized advice in the management of type 2 diabetes.
Sequence‐Dependent Repolarization Is Modulated by Endogenous Action Potential Duration Gradients Rather Than Electrical Coupling in Ventricular Myocardium
Authors: Blair Grace A. , Depman Madeline , Adams William P. , Maisonneuve Rowan O. , Hoeker Gregory S. , Weinberg Seth H. , Poelzing Steve 
Published: December 2024

Background
Previous studies suggest the relationship between activation time (AT) and action potential duration (APD) in the heart is dependent on electrotonic coupling, but this has not been directly tested. This study assessed whether acute changes in electrical coupling, or other determinants of conduction or repolarization, modulate APD heterogeneity.

Methods and Results
Langendorff‐perfused guinea pig hearts were epicardially paced and optically mapped after treatment with the gap junction uncoupler carbenoxolone, ephaptic uncoupler mannitol, ephaptic enhancer dextran 2MDa, sodium channel inhibitor flecainide, or rapid component of the delayed rectifier potassium channel inhibitor E4031. SD of APD and the AT–APD slope and coefficient of determination were quantified as metrics of APD heterogeneity. SD of APD increased with carbenoxolone, mannitol, and altered activation sequence. The AT–APD slope was insensitive to carbenoxolone, mannitol, dextran, flecainide, or E4031 but changed in response to activation sequence. The coefficient of determination did not change with carbenoxolone; decreased with mannitol, E4031, and activation sequence; but increased with dextran and flecainide. APD heterogeneity changes were dependent on whether the estimation used SD of APD or the AT–APD relationship. The pacing stimulus increased APD at the site of stimulation, revealing a confounding stimulus effect on APD within the measurement area. Simulations predict that the stimulus artifact and endogenous APD gradients are stronger determinants of APD heterogeneity than AT.

Conclusions
APD dependence on conduction is relatively small. Furthermore, APD heterogeneity within a mapping field of view is dependent on endogenous gradients, the stimulus artifact, and the experimental approach, rather than electrical coupling.