bluuebunny/crossref_metadata_2025_split huggingface dataset:
Raltegravir 1200 mg Once Daily as Maintenance Therapy in Virologically Suppressed HIV-1 Infected Adults : QDISS Open-Label Trial
Authors: Hall Nolwenn , Allavena Clotilde , Katlama Christine , Jobert Alexandra , Molina Jean-Michel , Cua Eric , Bani-Sadr Firouzé , Hocqueloux Laurent , Duvivier Claudine , Merrien Dominique , Hikombo Hitoto , André-Garnier Elisabeth , Gaultier Aurélie , Raffi François 
Published: August 2021

Abstract
Background: Raltegravir (RAL) has favorable tolerability and safety profile, with few and manageable drug interactions. The use of RAL 1200 mg once daily (qd) for first-line therapy is well established. We assessed efficacy and safety of RAL 1200 mg qd, as part of triple cART, for maintenance strategy.Methods: The QDISS trial (NCT03195452) was a 48-week multicenter, single-arm, open-label study designed to evaluate the ability of 2 NRTIs + RAL 1200 mg qd to maintain virological suppression in HIV-1 infected subjects on a stable cART with 2 NRTIs and a third agent for at least 6 months. The primary endpoint was the proportion of participants with HIV-1 RNA <50 copies/mL at week 24, by the FDA snapshot algorithm.Results: Of 100 participants 91% maintained viral suppression (95% CI: 83.6-95.8) at week 24 and 89% (81.2-94.4) at week 48. At week 24, there was one virological failure, without emergence of resistance-associated mutation and 10 participants had discontinued, 4 because of adverse events (AEs). Over 48 weeks, 7 AEs of grade 3-4 were reported, one possibly study-drug related (spontaneous abortion). BMI remained stable regardless of previous therapy or baseline BMI category. Over 48 weeks, total cholesterol (p=0.023) and LDL-cholesterol (p=0.009) decreased, lifestyle and ease subscale significantly improved (p=0.04). The quality of life and Patients Reported Outcomes (PROs) also improved at W12 (p=0.007). Conclusion: RAL 1200 mg qd as part of a maintenance triple therapy showed a high efficacy in virologically suppressed HIV-1 infected subjects, with good safety profile and improved lipid profile and patient reported outcomes.Trial registration = clinical trials.gov NCT03195452 and EudraCT 2016-003702-13
Genetic Structure and Diversity of Rice From Certified Cultivars and Farmer’s Fields in Burkina Faso
Authors: BARRO Mariam , KONATE Abdourasmane , WONNI Issa , KASSANKOGNO Abalo Itolou , SABOT François , ALBAR Laurence , SOMDA Irénée , BENA Gilles , GHESQUIERE Alain , KAM Honoré , SIE Moussa , CUBRY Philippe , TOLLENAERE Charlotte 
Published: August 2021

Abstract
Background: In West Africa, two rice species are cultivated, the African rice Oryza glaberrima and the Asian rice Oryza sativa, the second one being much more frequent. Despite its importance for food security in West Africa, the genetic diversity of Oryza sativa in farmer’s fields remains understudied in this region. Documenting the dynamics of diversity in the fields between landraces and improved cultivars is of importance to ensure rice cultivation adaptation to global change.In this study, we genotyped 77 rice samples from Burkina Faso using the C6AIR SNP array from IRRI. Among the studied samples, 27 were certified cultivars and 50 were sampled in rice fields from three geographical zones in western Burkina Faso, each zone comprising one irrigated area and a neighboring rainfed lowland. Obtained data were analyzed in the frame of the worldwide rice genetic diversity by using the 3K genomes as reference.Results: Most of the certified cultivars clustered with the indica genetic group, with a minority (26%) assigned to the japonica group. All except one of the rice samples from farmer’s fields belonged to the indica group. The peculiar one unexpectedly clustered with the Aus genetic group and originated from Tengrela (rainfed lowland in Karfiguela zone). This site, already known to differ in terms of agronomic practices, showed the highest genetic diversity compared to the five other sites, as well as highest genetic differentiation. Obtained genetic data confirmed the high global frequency of one cultivar, in accordance with the data from farmer’s interview. However, at the field level, genetic data rarely corresponded to the cultivar’s names obtained from farmer’s interviews.Conclusions: Overall we found a high genetic diversity in the studied samples from Burkina Faso (certified and field’s samples). We argue on the importance to document and preserve this agro-biodiversity as a prerequisite to face the current challenges of growing rice demand and global change. To this purpose, are required further scientific studies to refine our understanding of the dynamics of diversity in farmers’ fields, as well as a better knowledge of rice agro-biodiversity and awareness of its importance by rice farmers themselves.
A Three-Gene Based Diagnos Tic Model for Endometriosis
Authors: Fan Fangfang , Wei Qipeng , Xie Yue , Yin Xiaofang , Yang Xuezhou 
Published: August 2021

Abstract
Background: Endometriosis is a common gynecologic pathology among reproductive-aged women. The lack of a definitive clinical symptom and a minimally invasive diagnostic method lead to a diagnostic latency of endometriosis. In this study, we aim to establish a gene-based diagnostic model that can easily diagnose endometriosis. Results: Gene expression profiles of 226 tissue samples from the Gene Expression Omnibus and Genotype tissue expression databases were used to construct model for diagnosis of endometriosis. Using differential gene expression analysis, a total of 170 genes were identified. The weighted gene co-expression network analysis was utilized to identify hub genes. Lasso Cox regression was performed to identify diagnostic biomarkers and construct diagnosis model. Three genes including ELOVL6, UTP20 and ARHGAP18 were screened in the hub genes as the promising targets used for diagnosis of endometriosis. The three genes based diagnostic model was established by Least absolute shrinkage and selection operator and then validated in an independent validation cohort. Receiver operating characteristic curve analysis inferred the diagnostic model has a good performance in both training and validation cohort (The area under the curve of ROC achieved 0.75 and 0.73 in training and validation cohort, respectively). Conclusions: Therefore, our study established a novel three gene-based diagnostic model for endometriosis, which may assist clinicians in diagnosis of endometriosis.
Does Surgical Technique Influence the Burden of Lung Metastases in Patients with Pathologic Long Bone Fractures?
Authors: Kendal Joseph K. , Heard Bryan J. , Abbott Annalise G. , Moorman Scott W. , Saini Raghav , Puloski Shannon K. T. , Monument Michael James 
Published: August 2021

Abstract
Background: The aims of this study are to (1) determine whether fixation of metastatic long bone fractures with an intramedullary nail (IMN) influences the incidence of lung metastasis in comparison to arthroplasty or ORIF (Arthro/ORIF); and (2) assess this relationship in primary tumor types; and (3) to assess survival implications of lung metastasis after surgery.Methods: Retrospective cohort study investigating 184 patients (107 IMN, and 77 Arthro/ORIF) surgically treated for metastatic long bone fractures. Patients were required to have a single surgically treated impending or established pathologic fracture of a long bone, pre-operative lung imaging (lung radiograph or computed tomography) and post-operative lung imaging within 6 months of surgery. Primary cancer types included were breast (n=70), lung (n = 43), prostate (n = 34), renal cell (n = 37). Statistical analyses were conducted using two-tailed Fisher’s exact tests, and Kaplan-Meier survival analyses.Results: Patients treated with IMN and ORIF/arthroplasty developed new or progressive lung metastases following surgery at an incidence of 34% and 26% respectively. Surgical method did not significant influence lung metastasis (p = 0.33). Furthermore, an analysis of primary cancer subgroups did not yield any differences between IMN vs Arthro/ORIF. Median survival for the entire cohort was 11 months and 1-year overall survival was 42.7% (95% CI: 35.4-49.8). Regardless of fixation method, the presence of new or progressive lung metastatic disease at follow up imaging study was found to have a negative impact on patient survival (p <0.001).Conclusions: In this study, progression of metastatic lung disease was not affected by long bone stabilization strategy. IM manipulation of metastatic long bone fractures therefore may not result in a clinically relevant increase in metastatic lung burden. The results of this study also suggest that lung metastasis within 6 months of surgery for metastatic long bone lesions is negatively associated with patient survival. Level of Evidence: III, therapeutic study
Bioinformatic Analysis Identifies Biomarkers and Treatment Targets in Primary Sjögren's Syndrome Patients with Fatigue
Authors: Chen Guangshu , Che Li , Cai Xingdong , Zhu Ping , Ran Jianmin , Liu Shengming 
Published: August 2021

Abstract
We aim to identify the common genes, biological pathways and treatment targets for primary Sjögren's syndrome patients with varying degrees of fatigue features. We select datasets about transcriptomic analyses of Primary Sjögren's syndrome(pSS) patients with different degrees of fatigue features and normal controls in peripheral blood. We identify common differentially expressed genes(DEGs) to find shared pathways and treatment targets for pSS patients with fatigue and design protein-protein interactions (PPIs) network by some practical bioinformatics tools. And hub genes are detected based on the PPIs network. We perform biological pathways analysis of common genes by gene ontology(GO) terms and Kyoto Encyclopedia of Genes and Genomes(KEGG) pathway. Lastly, potential treatment targets for pSS patients with fatigue are found by the Enrichr platform. We discovered 27 DEGs are identified in pSS patients with fatigue features and the severe fatigued pSS-specific gene being RTP4. DEGs are mainly localized in the mitochondria, endosomes, endoplasmic reticulum, cytoplasm and are involved in the biological process by which interferon acts on cells and cells defend themselves against viruses. Molecular functions mainly involve the process of RNA synthesis. The DEGs of pSS are involved in the signaling pathways of viruses such as hepatitis C, influenza A, measles, and EBV. Acetohexamide PC3 UP, suloctidil HL60 UP, prenylamine HL60 UP, chlorophyllin CTD 00000324 are the four most polygenic drug molecules. PSS patients with fatigue features have specific gene regulation and chlorophyllin may alleviate fatigue symptoms in pSS patients.
ZC3H13 Can Serve as Potential Prognostic Biomarker and Therapeutic Target for Hepatocellular Carcinoma
Authors: wu Shuang , He Guifang , Liu Shihai , Cao Yongxian , Pan Huazheng 
Published: August 2021

Abstract
Background: Recently, an increasing number of studies have revealed that N6-methyladenosine (m6A) functions as a significant post-transcriptional modification which plays a critical role in the occurrence and progression of enriched tumors by regulating coding and non-coding RNA biogenesis. However, the roles of m6A RNA modification in hepatocellular carcinoma (HCC) remain largely unknown.Results: In this study, the expression levels, genetic variations, prognostic values and gene–gene interaction network of m6A-related genes in hepatocellular carcinoma were analyzed using the Oncomine, Gene Expression Profiling Interactive Analysis (GEPIA), cBioPortal, Kaplan–Meier plotter and GeneMANIA database. The results show that zinc finger CCCH-type containing 13 (ZC3H13) was significantly lowly expressed in Hepatocellular Carcinoma. Survival outcome analysis suggested that significant correlations occurred between ZC3H13 downregulation and poor overall survival (OS) and poor recurrence free survival (RFS) in patients with liver cancer. Whereupon, ZC3H13 was selected for analyzing the biological functions and pathways of its co-expressed genes via Metascape, and its correlation with immune system responses via the TISIDB database, respectively. Further analyses indicated that ZC3H13 may be involved in transcriptional misregulation in cancer. Additionally, ZC3H13 expression was significantly correlated with lymphocytes and immunomodulators. Conclusions: Therefore, ZC3H13 is a high candidate as a novel biomarker and therapeutic target for hepatocellular carcinoma.
Exosome-derived miR-127-5p Promotes Embryonic-like Stem Cells Differentiation Into Pacemaker Cell Through Nkx2.5 Down-regulation
Authors: Zhu Ye , You Jia , Gu Xiaojuan , Xu Chao , Gu Xiang 
Published: August 2021

Abstract
Available evidence suggests the involvement of microRNAs (miRNAs) in the pathological process of several diseases. Nonetheless, molecular mechanism underlying biological effects of miRNAs such as pacemaker exosome-derived miR-127-5p in embryonic-like stem cells (ESCs) differentiation into pacemaker cell is yet to be clarified. Through real time polymerase chain reaction (qPCR) or western blotting (WB) techniques, levels of miRNAs, miR-127-5p, and NKx2.5 expressions was quantitatively measured. Cellular differentiation (CD) was probed with flow cytometric (FC) and WB techniques. Prediction of miR-127-5p association with NKx2.5 was studied through bioinformatics tools before verification with luciferase assay. Promotion of ESCs differentiation to pacemaker through miR-127-5p was measured with qPCR and WB techniques. Through the same assaying methods, up-regulation of pace-making genes (shox2, HCN4, Cx45, Tbx3 and Tbx18) expression was observed in Nkx2.5 knockdown group. However, Nkx2.5 expression was down-regulated during differentiation of pacemaker-like cells into ESCs. Furthermore, techniques (such as qPCR, WB, immunofluorescent staining and FC) were employed to demonstrate that overexpressed miR-127-5p could suppress NKx2.5 expression. Through NKx2.5 targeting, ESCs could be differentiated into pacemaker-like cells with miR-127-5p possibly serving as a crucial positive regulator. On the account of our findings, further researches are needed to unearth the possible underlying mechanism and role of exosome-derived miRNAs in cell signaling.
Atmospheric Resuspension of Insoluble Radioactive Cesium Particles Found in the Difficult-to-Return Area in Fukushima
Authors: TANG PENG , Kita Kazuyuki , Igarashi Yasuhito , Satou Yukihiko , Hatanaka Koutarou , Adachi Kouji , Kinase Takeshi , Ninomiya Kazuhiko , Shinohara Atsushi 
Published: August 2021

Abstract
The deposition of insoluble radiocesium bearing microparticles (CsMPs), which were released from the Fukushima Daiichi Nuclear Power Plant (F1NPP) accident in March 2011, has resulted in the widespread contamination of eastern Japan. Obviously, these deposited insoluble CsMPs may become the secondary contamination sources by atmospheric migration or other environmental transferring process, however, the understanding of the transport mechanism remains non-elucidation, and the relevant evidence has not been directly provided. This study, for the first time, provides the direct evidence for the resuspension of these insoluble CsMPs to the atmosphere from 1) proximity of 137Cs radioactivity and resemblance of the morphology and the elemental compositions of CsMPs in the samples of soil and aerosol derived from the same sampling site, 2) the special characteristics of the resuspended CsMPs of which the ratios of Na/Si, K/Si and/or Cs/Si were smaller than those from the initially released CsMPs collected at either long distance or near F1NPP, which can be ascribed to the slowly natural corrosion of CsMPs by the loss of the small amount of soluble contents in CsMPs and 3) high CsMPs concentration of 10 granules/gram in the surface soil of our sampling site and the observed resuspension of surface soil dust at wind gust speed higher than 4 m s-1. Specifically, fifteen single CsMPs were successfully isolated from the aerosol filters collected by unmanned high-volume air samplers at a severely polluted area in Fukushima Prefecture, about 25 km away from F1NPP, from January 2015 to September 2019. The mean diameter of these CsMPs was 1.8 ± 0.5 μm, and the average 137Cs radioactivity was 0.35 ± 0.23 Bq/granule. The contribution rate of the resuspended CsMPs to the atmospheric radiocesium was estimated from the ratio of 137Cs radioactivity of a single CsMP to that of the aerosol filter to be of 23.9 ± 15.3%. There has been no considerable decreasing trend in the annual CsMP resuspension frequency.
Relation Between Personality Dimensions and Symptomatology of Depression in Skin Cancer Patients
Authors: Santos María Luisa Ramírez-de los , López-Navarro Araceli , Santos Saúl Ramírez-de los , Guzmán-Flores- Juan Manuel , Pereira-Suárez Ana Laura , Pulido Edgar Ivan Lopez 
Published: August 2021

Abstract
BackgroundEnvironmental psychological factors such as mood states can modify and trigger an organic response; depressive disorder is considered a risk factor for oncological development, leading to alterations both in the genesis and in the progression of the disease. Some authors have identified that personality relates to mood since a high score in neuroticism is associated with intense and long-lasting emotions of stress and therefore with the development of depressive behaviors. The objective of this study was to analyze the relationship between personality and depression in skin cancer patients. MethodsA total of forty-seven clinically and histopathologically diagnosed patients were scheduled for an hour-long interview, during which they provided informed consent and sociodemographic information. The psychological questionnaires applied were the revised Eysenck Personality Questionnaire and the clinical questionnaire for the diagnosis of the depressive syndrome. ResultsThe patient’s mean age was 66.5 years (SD ± 12.4) and the majority were diagnosed with basal cell carcinoma (70.2%). The frequency of anxious/depressive symptoms was 42.5%, with an increase in depression scores in the female gender (p <0.001). Furthermore, a difference was found in the neuroticism dimension related to gender, with higher values in women (p =0.002). Depressive symptomatologic portraits were correlated with the dimensions of neuroticism (p <0.001, r = 0.705), psychoticism (p =0.003, r = 0.422) and lying (p =0.028, r = -0.321). ConclusionsOur results support the hypothesis that personality dimensions are related to the presence of anxiety/depressive symptomatology in patients with skin cancer, especially in the female gender. Highlighting the need for future research that delves into the implications at the psychological level, the quality of life, and the biological mechanisms that link personality and depressive symptoms in the development and evolution of skin cancer.
Tyrosinase@HKUST-1: A Super Stable Biocatalyst Efficient for Catecholic Product Synthesis
Authors: Lü Xiao-Feng , Feng Chao-Yun , Li Shuangfei , Liu Guo-Hao , Yang Zhen 
Published: August 2021

Abstract
Although metal-organic frameworks (MOFs) have been considered as promising matrices for enzyme immobilization, HKUST-1, constructed from copper acetate (CuAc2) and benzene 1,3,5-tricarboxylate (BTC), has rarely been explored for this application. In this study, mushroom tyrosinase (EC 1.14.18.1) was immobilized in the form of tyrosinase@HKUST-1 following a simple reaction procedure we developed. The order of mixing BTC with the enzyme prior to addition of CuAc2 is believed to be responsible for the super stability exhibited by the immobilized enzyme so formed. The resultant biocatalyst was characterized in both structural features and catalytic properties. Upon incorporation into the HKUST-1 frameworks, the enzyme gained a prominent enhancement in stability against pH, temperature and storage: When incubated at 50 oC and pH 6.0, tyrosinase@HKUST-1 presented a half-life of 32.6 h, which is 77-fold and over 10-fold higher than that of the free enzyme and its other immobilization forms, respectively; and the catalyst fully maintained its activity for at least 2 months when stored at 30 oC. The applicability of this new biocatalyst was demonstrated by employing it as catalyst for regioselective ortho-hydroxylation reactions to produce catecholic products with huge pharmacological effects, i.e., hydroxytyrosol and L-DOPA, with excellent yields and productivities. This study has thus offered a facile immobilization method to prepare a novel biocatalyst with super stability, and tyrosinase@HKUST-1 so formed from crude mushroom extract provides an efficient catalyst which can be applied to the production of catecholic products with health benefits.
Multi-objective Optimization of Compliant Parallel Platform for Desired Stiffness and Workspace
Authors: Xu Hongwei , Zhou Haibo , Li Zhiqiang , Ju Xia 
Published: August 2021

Abstract
Stiffness and workspace are crucial performance indexes of a precision mechanism. In this paper, an optimization method is presented, for a compliant parallel platform to achieve desired stiffness and workspace. First, a numerical model is proposed to reveal the relationship between structural parameters, desired stiffness and workspace of the compliant parallel platform. Then, the influence of the various parameters on stiffness and workspace of the platform is analyzed. Based on Gaussian distribution, the multi-objective optimization problem is transformed into a single-objective one, in order to guarantee convergence precision. Furthermore, particle swarm optimization is used to optimize the structural parameters of the platform, which significantly improve its stiffness and workspace. Last, the effectiveness of the proposed numerical model is verified by finite element analysis and experiment.
Oblique Lateral Interbody Fusion Combined With Lateral Plate Fixation for the Treatment of Lumbar Degenerative Diseases: a Preliminary Clinical Study
Authors: Li Hai-dong , Xing Shi-Tong , Min Ji-Kang , Fang Xiang-Qian , Fan Shun-Wu 
Published: August 2021

Abstract
Background The oblique lateral interbody fusion (OLIF) is a minimally invasive indirect decompression technique for the treatment of lumar spine disease. The OLIF has usually combined with supplemental posterior pedicle screw fixation for decreasing the perioperative complications. The purpose of this study was to evaluate clinical efficacy and complications of oblique lateral interbody fusion (OLIF) combined with lateral plate instrumentation for the treatment of lumbar degenerative diseases.Methods From May 2020 to August 2020, the clinical data of 20 patients who underwent OLIF combined with lateral plate instrumentation were retrospectively analyzed. The operation time, blood loss, and the complications were recorded. Also, the radiological parameters, Visual Analog Scale (VAS) score and Oswestry Disability Index (ODI) were evaluated before and after surgery.Results The average operation time, blood loss, and length of hospital stay were 75.41±11.53 min, 39.57±9.22 ml, and 7.22±1.85 days, respectively. The VAS and ODI had both significantly improved after surgery (7.23±1.26 VS 2.15±0.87; 60.27±7.91 VS 21.80±6.32, P < 0.001). The postoperative disk height (DH) was 13.02±8.83 mm, which is much higher than before (P < 0.001); The postoperative foraminal height (FH) improved significantly (16.18±3.49 VS 21.54±2.12 mm, P < 0.01), and the cross-sectional area (CSA) had improved from 88.95±14.79 mm2 to 126.53±8.83 mm2 (P < 0.001). The radiological fusion rate was 95% at the last follow-up, while cage subsidence was found in one case. No major complications, such as ureteral injury, vascular injury and vertebral body fracture occurred.Conclusions OLIF combined with lateral plate fixation can avoid the lumbar posterior surgery, minimize the operation time, blood loss and the posterior ligament complex disruption. It can realize one-stage intervertebral fusion and instrumentation through a single small incision.