bluuebunny/crossref_metadata_2025_split huggingface dataset:
Chemical pre-treatments on oil palm empty fruit bunches: Impacts on characteristics and methane potential
Authors: Rohma N A , Suhartini S , Nurika I 
Published: December 2021

AbstractProduction of biogas from lignocellulosic biomass by anaerobic digestion (AD) has attracted much interest. Oil palm empty fruit bunches (OPEFB), one of lignocellulosic biomass, is highly abundant in Indonesia and has potential as feedstock for bioenergy production such as biogas or methane. Yet, pre-treatments are needed to improve biogas production due to its complex crystalline structures. Chemical pre-treatments with acid or alkaline solution were reported to increase cellulose or highly reduce the lignin content of OPEFB. This study aimed to evaluate the effect of acid and alkaline pre-treatments on the characteristics of OPEFB and methane potential. The acid pre-treatment experimental design was used factor of H2SO4concentration (1, 1.3, and 1.6 (%v/v)) and NaOH concentration (1.8, 2.8, and 3.8 (%w/v)). Methane potential evaluation was carried out using the biochemical methane potential (BMP) test with the Automatic Methane Potential Test System (AMPTS) II under mesophilic condition (37°C), operated for 28 days. The results showed that both dilute acid and alkaline pre-treatment positively impact altering the characteristics of OPEFB, hence the specific methane potential. Alkaline pre-treatment with NaOH 3.8 (%w/v) gave the highest average SMP value of 0.161 ± 0.005 m3CH4/kgVSadded.
Bioconversion of lignin and methane production from Corn cobs (Zea mays) treated by lignin-degrading bacteria
Authors: Nurika I , Rahmadhanti A , Suhartini S 
Published: December 2021

AbstractCorn cobs are one of the potential feedstocks consisting of cellulose, hemicellulose and lignin, which provide potential lignocellulose biomass to be converted into renewable energy such as biogas through anaerobic digestion (AD). However, the recalcitrant structure of corn cobs lignocellulose makes it resistant to microbial access to the cell wall, and therefore the effective pre-treatment needs to be conducted. The biological pre-treatment using lignin-degrading bacteria is one of the promising bioconversion processes which will help to break down the lignocellulose structure. This study aims to analyse the ability of bacteria,Agrobacterium sp., Lysinibacillus sphaericusandPaenibacillus sp. in degrading lignin of corn cobs and therefore will enhance the methane released from AD. The ability of bacteria to degrade lignin was observed by analysis of total reducing sugar, total soluble phenols, lignin content, and weight loss, while the methane production was determined by the biochemical methane potential (BMP). The percentage of lignin content of untreated and pre-treated corn cobs with bacteriaAgrobacterium sp., L. sphaericusandPaenibacillus sp. is 18.34%; 9.66%; 11.48% and 9.06%, respectively. The methane concentration (specific methane production) produced by using inoculum of Agrobacteriumsp., L. sphaericusandPaenibacillus sp. with the addition of pre-treated corn cobs are 1.79%; 1.16% and 2.51%, respectively. These results were higher than the inoculum with the addition of untreated corn cobs.
Optimization of dissolved proteins in extracts of earthworm (Lumbricus rubellus) with factor adding concentration of papain enzyme and earthworms
Authors: Hidayat N , Nugrahany Y D , Permatasari V R , Nurika I 
Published: December 2021

Abstract
Earthworms (Lumbricus rubellus) can be used as an alternative to meet protein needs. This study aimed to obtain optimal N-Amino and Total Soluble Solid (TSS) results in earthworm extract. Efforts to improve the results of N-Amino and TDS (Total Dissolved Solids) in earthworm extract can be done by hydrolyzing the juice of earthworms with the help of enzyme protease papain under certain conditions, so it will facilitate the process of solving protein content. The experimental design in this study was Surface Methodology Response (RSM) model using thirteen models and two factors, namely the addition of papain enzyme concentration (6%, 8% and 10% (b/v)) and the percentage of earthworm base material (10%, 20% and 30% (b/v)). The results of this study showed the highest total protein value of 47.93% (g/L) obtained at the concentration of earthworms by 30% and 10% of papain enzyme. Then the validation results showed the optimum solution at 30% concentration of earthworms and 10% of papain enzyme that produced an N-Amino response of 7.2% and a TDS of 74% (g/L) with a Desirability of 0.906. N-Amino has a quadratic model with the actual variable equation

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Optimization of fermentation time and grain concentration for water kefir production from butterfly pea flower (Clitoria ternatea)
Authors: Setiawati A E , Kusnadi J 
Published: December 2021

Abstract
Herbal tea is known rich in bioactive compounds and pigments. Herbal tea can be derived from Butterfly pea flowers. Butterfly pea has a high level of anthocyanins and flavonoid levels. However, butterfly pea has not been used optimally in fermentation products such as water kefir. Water kefir has many benefits and potential antioxidant sources. This research aimed to optimize fermentation time (24-48 hours) and concentration grain (5-15%) for the production of butterfly pea water kefir with a good antioxidant activity using response surface methodology. Central Composite Design was used to develop models of the response. The response used in this research is antioxidant activity, flavonoid, anthocyanin and brightness. The optimum condition was obtained at 36 hours 2 minutes of fermentation time, and 15% of grain concentration using Design Expert 10.01. The optimum combination for butterfly pea water kefir resulted in 58 ppm of antioxidant activity, 39 of brightness, 81 mg/g QE of flavonoid and 4.2 ppm of anthocyanin. A validation experiment showed that no significant difference between actual and predicted values based on paired t-test (p>0.05) using Minitab 17.0.
Antimicrobial activity of bacteriocin like inhibitory substance (BLIS) and lactic acid bacteria (LAB) isolated from traditional fermented buffalo milk from West Sumatra, Indonesia
Authors: Hayati F , Yuliana T , Rialita T 
Published: December 2021

Abstract
Food preservation by chemical preservatives addition is not recommended in large quantities. However, lactic acid bacteria (LAB) metabolites can be used as a safe alternative. LAB can produce a type of antimicrobial peptide known as bacteriocin. One source of LAB that can be used as a bacteriocin-producing candidate is dadih, a traditional fermented buffalo milk product from West Sumatra, Indonesia. In this study, eight bacterial isolates isolated from dadih were identified using the catalase test and Gram straining and obtained 6 isolates that were taken as LAB. An antimicrobial activity test was carried out using the diffusion method on LAB liquid isolates suspension and crude bacteriocins from the six isolates. The isolates with the highest antimicrobial activity were DK8, the diameter of inhibiting zone of liquid isolates was 15.75 mm (Salmonella sp.), 14.25 mm (Escherichia coli), and 12.25 mm (Staphylococcus aureus). Meanwhile, the diameter of crude bacteriocin inhibition of DK 8 isolates was 8 mm (Salmonella sp.). 8 mm (E. coli), and 8.5 mm (S. aureus). Furthermore, 16S rRNA sequencing confirmed DK8 as Lactobacillus pentosus.
The role of carbon and nitrogen sources in the production of bioactive compounds in Monascus fermentation products: a mini review
Authors: Widayanti V T , Estiasih T , Zubaidah E , Taher M 
Published: December 2021

Abstract
Angkak is one of the Monascus fermented products with rice as a substrate. In addition, Chinese yam, potatoes, soybean, ginseng and onions can be used as a substrate for fermentation products by Monascus sp. It was known that the fermented product by Monascus sp. produces several bioactive compounds that have antihyperlipidemic activity. These bioactive compounds are monacolin-K and pigment compounds (monascin and ankaflavin). Each of these compounds has different cholesterol inhibitory activity. The production of these bioactive compounds is strongly influenced by the nutritional composition of the fermentation media. The addition of the right carbon and nitrogen sources can accelerate the production of bioactive compounds by Monascus sp.
Extraction and characterization of nano calcium from tilapia (Oreochromis niloticus) scales from Semarang, Indonesia
Authors: Dewi F R 
Published: December 2021

Abstract
The research aims to develop a method to extract nano calcium from tilapia (Oreochromis niloticus) scales. Yielding nano calcium from the fish scale will enable the marine and fisheries community to gain a higher economic value from this untapped source. The first study was designed to find the best acid solution (citric acid and HCl) and time (3 and 6h) for the extraction. The result revealed that calcium extracted with HCl for 3 h had a higher calcium content and yield than other treatments. The following study was conducted to find the best concentration of HCl and time for the extraction. The result revealed that calcium extracted for 30 mins with HCl 0.3 N had calcium content higher than other treatments. The powder is then ground with high energy milling (HEM) at a different time (3 and 2 h, and 15, 30, 35, 60, 75 mins) to get nano-sized particles. The Morphology and elements were analyzed using scanning electron microscopy (SEM) and energy dispersive X-ray (EDX). To conclude, extracting with 0,3 N HCl for 60 mins and with a milling time for 45 mins was the best method to extract nano calcium from tilapia scales. The nano calcium can be used as additional ingredients for snacks to add value.
The effect of NaOH addition on the characteristics of tilapia skin collagen
Authors: Nurhayati  , Kusumawati R , Suryanti  
Published: December 2021

Abstract
Research on fish collagen is now growing rapidly as the use of collagen in industry increases. Collagen extraction begins with the removal of non-collagen proteins using bases to maximize the extraction process. This research aims to determine the effect of differences in NaOH concentrations on the characteristics of tilapia skin collagen. NaOH in collagen extraction serves to remove alkaline soluble proteins to optimize the collagen extraction process. The bases used were NaOH with the concentration of 0.5, 1.0, and 1.5%. The extraction was carried out using the acid method. Using SEM, observation parameters for crude collagen from the tilapia skin include collagen yield, functional group analysis, lightness, and surface morphology. The results of functional groups analysis showed that the collagen obtained in all treatments had typical collagen characteristics, i.e., amide A, amide B, amide I, amide II, and amide III. The non-collagen deproteination treatment with 0.5% NaOH could produce better collagen than the 1.0 and 1.5% concentrations, as indicated by the highest yield (20.42%) and lightness (93.22). Morphological analysis showed that the collagen extracted has an irregular branched fiber structure.
Influence of methods and norms of sowing soybean seeds of the Northern ecotype on yield
Authors: Fadeev A , Nikiforova I , Ivanova I 
Published: December 2021

Abstract
The article presents the results of studying the optimal norms and methods of sowing a new variety of soybeans of the northern ecotype of the Memory of Fadeev for 2016-2018 at the experimental site of the Chuvash Research Agricultural Institute-a branch of the Federal State Budgetary Institution “Federal Agricultural Research Center of the North-East named after N V Rudnitsky” in the southern part of the Volga-Vyatka region of Russia. It is established that in order to obtain high yields of soybean seeds of the northern ecotype in the conditions of the Chuvash Republic, the ultra-ripe variety of soy in Memory of Fadeev should be sown in a wide-row way at a rate of 600 thousand germinating seeds per 1 ha. It is revealed that to obtain a higher attachment of the lower bean, narrow-row sowing is recommended - the height of the attachment increases to 17.3 cm. Wide-row sowing increases the number of beans, the number and weight of seeds per plant, as well as the weight of 1000 seeds, which leads to an additional yield of up to 6.8 c/ha. According to the data obtained, an agroecological passport has been developed for a new Chuvash soybean variety of the northern ecotype of the Fadeev Memory variety, zoned in 2019.
Influence of fertilizers on the productivity of tea plants in the conditions of the north-west Caucasus
Authors: Lagoshina A , Pchihachev E , Chernjavskaja I , Aleksandrova A , Belous O 
Published: December 2021

AbstractThe research is aimed at finding effective ways to regulate the functional state of tea plants under stress, increase in yield and its stability, preserving and improving the quality. Foliar spraying of the tea stimulated the active formation of Proline (135.1-179.2 mg/g, in the control - 122.6 mg/g) and ascorbic acid (177.8-226.5 mg/g, in the control - 131.4 mg/g) in the leaves, which indicates the inclusion of processes associated with the mechanism of nonspecific protection against stress. The optimal state of tea plants treated with fertilizers explains the more developed specific surface density of the leaf (1.44 mg/cm2, in the control -1.24 mg/cm). The developed specific surface density of the leaf was ensuring the active operation of the leaf apparatus on the experimental variants, which is expressed in a higher productivity of the leaves (1.27-1.31 g/dm2), compared to the control plants. The highest yield (about 23.05 centner per ha) was observed in the variant with foliar treatments with rokogumin, in the variant with sodium humate (31.47 centner per ha), the yield exceeded the control (26.88 centner per ha) only in 2020, which may be due to the accumulative effect.
Milk productivity of holstein cows at optimization of fat levels in their diets
Authors: Petrov O , Semenov V , Alekseev V 
Published: December 2021

Abstract
The work is devoted to determining the optimal level of fat in concentrate-senage diets for high-yield cows and studying the effect of various levels of fat in the dry matter of the diet on milk productivity and chemical composition of milk. Studies have shown the insufficiency of household diets of highly productive cows with a dry diet content of 3.2% raw fat. This deterred the potential for dairy productivity. Addition of fat in cow diets by equivalent energy and protein replacement of a portion of the compound feed with a rapeseed cake up to a level of 4.2% of the dry substance provides an increase in milk productivity in relation to the control group receiving 3.2% fat. Optimization of the fat level in the dry substance of the diet up to 4.2% in the nutrition system of cows increases the functional activity of the breast in their body, which is expressed in the growth of breast productivity by 6.78%, improvement of the chemical composition and technological properties of milk. A further increase in dietary fat to 5.2% has a less pronounced effect on the change in milk productivity.
Effect of preparation Dihydroquercetin on growth-weight indices of broilers
Authors: Kuzmina N N , Yu Petrov O , Semenov V G , Boronin V V 
Published: December 2021

Abstract
Research is devoted to the study of the feasibility of using a natural antioxidant of plant origin as a biologically active additive to the basic diet and assessing the effectiveness of its use to increase the meat productivity of broiler chickens. In our work we examined the broiler chickens productivity and quality when using the drug ‘Dihydroquercetin’ (LLC’Kakhor’, Zima, Irkutsk region, Russia) in diets and established the amount of feed consumed and its costs per unit of production. Based on the studies, the addition of the preparation enables to increase the safety of broiler chickens by 20…30%, live mass - by 12…33%, mass of ripped carcass - by 15…38%, muscle tissue - by 3%, thoracic muscles - by 0.3…2%, and edible parts of carcass -by 2…6%. Moreover, the additive reduces the feed consumption by a head during the fattening period by an average of 46.09 g compared to the control.
The effect of fumaric acid, dipromonium and vitamin C on the productivity of sows
Authors: Solyanik A V , Semenov V G , Tyurin V G , Kuznetsov A F , Sofronov V G , Kh Volkov A , Solyanik V V , Solyanik S V , Solyanik V A 
Published: December 2021

Abstract
The article presents the results of the effect of fumaric acid, dipromonium and vitamin C on productivity, physiological state and natural resistance of sows. The scientific and economic experience was carried out at the pig-breeding complex with a production capacity of 27 thousand heads “Voskhod” in the Mogilev region of Belarus. Forty primiparous gilts were divided into four groups (n = 10): a control and three experimental groups. Experimental gilts received basal diet supplemented with fumaric acid (4.0 g/kg of dry matter), dipromonium (0.4 g/kg) or vitamin C (0.1 g/kg) from 1 to 20 d of lactation. The animals of the control group did not receive the indicated preparation. The use of additives contributed to an increase in the milk yield of pigs, the viability of piglets and their growth rate by 21.1-30.0% (P<0.05), 6.0-6.6 and 10.0-27.9%, respectively, as well as influenced the morphological, biochemical and immunological parameters of the blood of pigs and their progeny.
Kinematics and dynamics of an incomplete circular wheel drive of an agricultural tractor
Authors: Kazakov Yu , Medvedev V , Batmanov V , Pavlov V 
Published: December 2021

Abstract
The disadvantage of wheeled tractors is soil compaction, slipping due to limited traction, low tangential force. Experimental studies of a tractor with incomplete circular wheel mover on stubble, sand and virgin snow showed an increase in cross-country ability, a decrease in skidding, an increase in traction, and an increase in productivity. The purpose of the study is to develop a methodology for kinematic and dynamic analysis of incompletely rounded wheel propellers with a built-in differential. The equation of motion of the wheel is obtained on the basis of two-stage overcoming by the wheel of a single threshold obstacle taking into account the longitudinal and radial stiffness of the tire, its deformation, air resistance in the tire. The main influence is provided by translational speed, wheel radius and radial stiffness, the moment of inertia of the wheel and the shoulder of the application of mass. Planetary gearbox proposed in which the shaft of the driving satellite is a bearing, while the radius of the gear is an order of magnitude smaller than the radius of the wheel. The direction of improvement of wheel mover, increasing their traction properties is justified.
Influence of the type of higher nervous activity on milk yield and the productive longevity of mares
Authors: Chirgin E D , Semenov V G , Ivanova E N 
Published: December 2021

Abstract
The presented work is the result of studies of the influence of the type of higher nervous activity on the dairy productivity of mares of the Russian heavy draft horse breed on stationary koumiss farms. To determine the types of higher nervous activity, a motor-food technique developed by the All-Russian Research Institute of Horse Breeding was used. The milk yield was counted at animals for the first lactation, for the highest lactation, on average for one lactation and a lifelong milk yield of mares. The milk yield on average for lactation is almost the same in mares with a strong balanced mobile type and with a strong unbalanced type of higher nervous activity. Animals of a weak type and a strong balanced inert type of higher nervous activity lag behind them in this indicator by 11-13%. Horses with a strong balanced mobile type, a strong unbalanced type and a weak type of higher nervous activity are most suitable for the duration of economic use and lifelong milk production. The mares with a strong balanced inert type of higher nervous activity are the least productive in terms of duration of economic use and lifelong milk yield on koumiss farms.
The influence of stallions on the properties of the udder of daughters
Authors: Chirgin E D , Semenov V G , Tyurin V G , Nikitin D A , Biryukov K N 
Published: December 2021

Abstract
The presented work is the result of studies of the influence of the linearity of mares of the Russian heavy draft breed on milk productivity, morphological characteristics of the udder and adaptability to machine milking. We examined mares belonging to four lines of stallions: Day laborer, Casket, Svist and Karaul. The calculated milk yield on average per lactation was the highest in mares of the Svist line, and the lowest in the Larchik line. Lifetime milk yield of mares of the Svist line exceeded the same indicator of mares of the Podennik line by 12.13%, of the Karaul line - by 18.51% and the Larchik line - by 47.77%. Studies have shown that Whistle stallions not only contribute to the high milk production of their daughters, but also impart good adaptability to machine milking. Mares of the Larchik line in this population of horses of the Russian heavy draft breed are the least productive and the least adapted to machine milking.
Effect of immunoprophylaxis on reproduction function of highly productive cows
Authors: Kondruchina S G , Semenov V G , Tyurin V G , Kuznetsov A F , Leontyev L B , Larionov G A , Ivanova T N , Tolstova S L , Biryukova D E , Stepanova A V , Semenov A A , Ivanova E N 
Published: December 2021

Abstract
The article describes a method of preventing postpartum obstetric-gynecological diseases and improving cow reproductive function due to the increase of body nonspecific resistance with the application of electropuncture and biopreparation Prevention-N-C (Chuvash State Agrarian University, Russia) and Salus-PE (Chuvash State Agrarian University, Russia). The cows of the 1st group were injected three times 45-40, 25-20 and 15-10 days before calving intramuscularly at a dose of 10 ml/head with the biopreparation Prevention-N-C, and the cows of the 2nd group were injected according to the same scheme and the same term with Salus-PE, and the cows of the 3nd group electropuncture was performed on the Vocal-B device (BIORS, Russia) immediately after childbirth, three times, after 48 h. The use of biopreparations during critical periods of cow steeliness reduced the risks of subinvolution of the uterus, endometritis and mastitis in the postpartum period and increased the reproductive qualities of cows. It has been shown that after the use of Salus-PE, 100% of cows are fertilized, 60% of which are fertilized in the first sexual hunt, and 40% - in the second. The insemination index was 1.4.
Formation of colostral immunity in calfs on the background of the application of immunostimulators to cows
Authors: Semenov V G , Matveeva E S , Biryukova D E , Maykotov A N , Kondruchina S G , Ivanova T N , Musaev S A , Tolstova S L , Lukina N M , Zaharovskiy G V 
Published: December 2021

Abstract
A scientific and practical substantiation of the need for the timely formation of colostral immunity in calves to create nonspecific protection of the body and adaptation of newborns to new environmental conditions is given, which is the most important problem of modern veterinary science and practice. Colostral immunity is immunity that develops in newborns due to colostral immunoglobulins during the first 24-36 hours of life. For some newborn farm animals (horses, ruminants and pigs), antibodies are transmitted to offspring only through colostrum in the early postnatal period. In order to increase the nonspecific defenses of the body of mothers cows, the formation of colostral immunity in newborn calves and the realization of the biological resource potential of the body, immunostimulants were used, developed by scientists of the Chuvash State Agrarian University: Prevention-N-B-S and Salus-PE.