bluuebunny/crossref_metadata_2025_split huggingface dataset:
VAMP-2 is a surrogate cerebrospinal fluid marker of Alzheimer-related cognitive impairment in adults with Down syndrome
Authors: Lleó Alberto , Carmona-Iragui Maria , Videla Laura , Fernández Susana , Benejam Bessy , Pegueroles Jordi , Barroeta Isabel , Altuna Miren , Valldeneu Silvia , Xiao Mei-Fang , Xu Desheng , Núñez-Llaves Raúl , Querol-Vilaseca Marta , Sirisi Sònia , Bejanin Alexandre , Iulita M. Florencia , Clarimón Jordi , Blesa Rafael , Worley Paul , Alcolea Daniel , Fortea Juan , Belbin Olivia 
Published: June 2021

AbstractBackgroundThere is an urgent need for objective markers of Alzheimer’s disease (AD)-related cognitive impairment in people with Down syndrome (DS) to improve diagnosis, monitor disease progression, and assess response to disease-modifying therapies. Previously, GluA4 and neuronal pentraxin 2 (NPTX2) showed limited potential as cerebrospinal fluid (CSF) markers of cognitive impairment in adults with DS. Here, we compare the CSF profile of a panel of synaptic proteins (Calsyntenin-1, Neuroligin-2, Neurexin-2A, Neurexin-3A, Syntaxin-1B, Thy-1, VAMP-2) to that of NPTX2 and GluA4 in a large cohort of subjects with DS across the preclinical and clinical AD continuum and explore their correlation with cognitive impairment.MethodsWe quantified the synaptic panel proteins by selected reaction monitoring in CSF from 20 non-trisomic cognitively normal controls (mean age 44) and 80 adults with DS grouped according to clinical AD diagnosis (asymptomatic, prodromal AD or AD dementia). We used regression analyses to determine CSF changes across the AD continuum and explored correlations with age, global cognitive performance (CAMCOG), episodic memory (modified cued-recall test; mCRT) and CSF biomarkers, CSF Aβ42:40ratio, CSF Aβ1-42, CSF p-tau, and CSF NFL. P values were adjusted for multiple testing.ResultsIn adults with DS, VAMP-2 was the only synaptic protein to correlate with episodic memory (delayed recalladj.p= .04) and age (adj.p= .0008) and was the best correlate of CSF Aβ42:40(adj.p= .0001), p-tau (adj.p <.0001), and NFL (adj.p <.0001). Compared to controls, mean VAMP-2 levels were lower in asymptomatic adults with DS only (adj.p= .02). CSF levels of Neurexin-3A, Thy-1, Neurexin-2A, Calysntenin-1, Neuroligin-2, GluA4, and Syntaxin-1B all strongly correlated with NPTX2 (p< .0001), which was the only synaptic protein to show reduced CSF levels in DS at all AD stages compared to controls (adj.p< .002).ConclusionThese data show proof-of-concept for CSF VAMP-2 as a potential marker of synapse degeneration that correlates with CSF AD and axonal degeneration markers and cognitive performance.
Comparison of chondrogenesis-related biological behaviors between human urine-derived stem cells and human bone marrow mesenchymal stem cells from the same individual
Authors: Sun Jiachen , Xing Fei , Zou Min , Gong Min , Li Lang , Xiang Zhou 
Published: June 2021

Abstract
Background
Stem cells are the main choice for seed cells in tissue engineering, but using most traditional stem cells requires invasive and complicated procedures. Human urine-derived stem cells (hUSCs) are an alternative stem cell source with the advantages of being isolated noninvasively and repetitively from the same individual. The aim of this study was to compare chondrogenesis-related biological behaviors between hUSCs and human bone marrow mesenchymal stem cells (hBMSCs) from the same individual.

Methods
hUSCs and hBMSCs were isolated from six patients who underwent iliac bone grafting. Cell morphology, proliferation, colony-forming, migration, and multidifferentiation analyses were performed in vitro. Then, acellular cartilage extracellular matrix (ACM) scaffolds were fabricated for in vivo implantation. The comparisons of cell viability, morphology, proliferation, and chondrogenesis between hUSCs and hBMSCs cultured on scaffolds were performed before implantation. The scaffolds loaded with hUSCs or hBMSCs were implanted into a rabbit knee model to repair cartilage defects. Magnetic resonance imaging (MRI) and micro-computed tomography (μCT) Analyses, inflammation and toxicity assays, gross observation, and histological evaluation were performed to evaluate the cartilage repair effects.

Results
In in vitro experiments, hUSCs had better capacity for proliferation, colony-forming, and migration compared to hBMSCs in the same passage, while hBMSCs had greater osteogenic, adipogenic, and chondrogenic abilities compared to hUSCs in the same passage. Both hUSCs and hBMSCs at passage 3 had the strongest potential for proliferation, colony-forming, and multilineage differentiation compared to cells in other passages. The ACM scaffolds loaded with hUSCs or hBMSCs both significantly promoted the repair of cartilage defects in the rabbit knee model at 12 weeks’ postimplantation, and the new tissue was mainly hyaline cartilage. However, there was no significant difference in cartilage repair effects between hUSCs and hBMSCs.

Conclusions
In in vitro experiments, hUSCs presented better capacity for proliferation, while hBMSCs had greater chondrogenic ability. However, hUSCs and hBMSCs had similar cartilage repair effects in vivo. Results indicated that hUSCs can be a stem cell alternative for cartilage regeneration and provide a powerful platform for cartilage tissue engineering and clinical transformation.

Graphical abstract
Hypoxic mesenchymal stem cells ameliorate acute kidney ischemia-reperfusion injury via enhancing renal tubular autophagy
Authors: Tseng Wei-Cheng , Lee Pei-Ying , Tsai Ming-Tsun , Chang Fu-Pang , Chen Nien-Jung , Chien Chiang-Ting , Hung Shih-Chieh , Tarng Der-Cherng 
Published: June 2021

Abstract
Background
Acute kidney injury (AKI) is an emerging global healthcare issue without effective therapy yet. Autophagy recycles damaged organelles and helps maintain tissue homeostasis in acute renal ischemia-reperfusion (I/R) injury. Hypoxic mesenchymal stem cells (HMSCs) represent an innovative cell-based therapy in AKI. Moreover, the conditioned medium of HMSCs (HMSC-CM) rich in beneficial trophic factors may serve as a cell-free alternative therapy. Nonetheless, whether HMSCs or HMSC-CM mitigate renal I/R injury via modulating tubular autophagy remains unclear.

Methods
Renal I/R injury was induced by clamping of the left renal artery with right nephrectomy in male Sprague-Dawley rats. The rats were injected with either PBS, HMSCs, or HMSC-CM immediately after the surgery and sacrificed 48 h later. Renal tubular NRK-52E cells subjected to hypoxia-reoxygenation (H/R) injury were co-cultured with HMSCs or treated with HMSC-CM to assess the regulatory effects of HSMCs on tubular autophagy and apoptosis. The association of tubular autophagy gene expression and renal recovery was also investigated in patients with ischemic AKI.

Result
HMSCs had a superior anti-oxidative effect in I/R-injured rat kidneys as compared to normoxia-cultured mesenchymal stem cells. HMSCs further attenuated renal macrophage infiltration and inflammation, reduced tubular apoptosis, enhanced tubular proliferation, and improved kidney function decline in rats with renal I/R injury. Moreover, HMSCs suppressed superoxide formation, reduced DNA damage and lipid peroxidation, and increased anti-oxidants expression in renal tubular epithelial cells during I/R injury. Co-culture of HMSCs with H/R-injured NRK-52E cells also lessened tubular cell death. Mechanistically, HMSCs downregulated the expression of pro-inflammatory interleukin-1β, proapoptotic Bax, and caspase 3. Notably, HMSCs also upregulated the expression of autophagy-related LC3B, Atg5 and Beclin 1 in renal tubular cells both in vivo and in vitro. Addition of 3-methyladenine suppressed the activity of autophagy and abrogated the renoprotective effects of HMSCs. The renoprotective effect of tubular autophagy was further validated in patients with ischemic AKI. AKI patients with higher renal LC3B expression were associated with better renal recovery.

Conclusion
The present study describes that the enhancing effect of MSCs, and especially of HMCSs, on tissue autophagy can be applied to suppress renal tubular apoptosis and attenuate renal impairment during renal I/R injury in the rat. Our findings provide further mechanistic support to HMSCs therapy and its investigation in clinical trials of ischemic AKI.
Diaphragm dysfunction, lung aeration loss and weaning-induced pulmonary oedema in difficult-to-wean patients
Authors: Dres Martin , Rozenberg Emmanuel , Morawiec Elise , Mayaux Julien , Delemazure Julie , Similowski Thomas , Demoule Alexandre 
Published: June 2021

Abstract
Background
Diaphragm dysfunction and weaning-induced pulmonary oedema are commonly involved during weaning failure, but their physiological interactions have been poorly reported. Our hypothesis was that diaphragm dysfunction is not particularly associated with weaning-induced pulmonary oedema.

Methods
It was a single-centre and physiological study conducted in patients who had failed a first spontaneous breathing trial and who underwent a second trial. The diaphragm function was evaluated by measuring the tracheal pressure generated in response to a bilateral magnetic phrenic nerves stimulations. Weaning-induced pulmonary oedema was diagnosed in case of failure of the spontaneous breathing trial if patients exhibited signs of plasma concentration or echocardiographic diagnosis of pulmonary artery occlusion pressure elevation.

Results
Fifty-three patients were included and 31/53 (58%) failed the spontaneous breathing trial, including 24/31 (77%) patients with weaning-induced pulmonary oedema. Diaphragm dysfunction was present in 33/53 (62%) patients. Diaphragm dysfunction or weaning-induced pulmonary oedema were present in 26/31 (84%) of the patients who failed the spontaneous breathing trial. Weaning-induced pulmonary oedema occurred in 20/33 (61%) patients with a diaphragm dysfunction and in 4/20 (20%) patients without (p = 0.005).

Conclusion
Weaning-induced pulmonary oedema was three times more frequent in case of diaphragm dysfunction. Even in case of diaphragm dysfunction, physicians might be encouraged to investigate the presence of weaning-induced pulmonary oedema during weaning failure.
Revisiting Samuelson’s models, linear and nonlinear, stability conditions and oscillating dynamics
Authors: Tramontana Fabio , Gardini Laura 
Published: June 2021

AbstractIn this work, we reconsider the dynamics of a few versions of the classical Samuelson’s multiplier–accelerator model for national economy. First we recall that the classical one with constant governmental expenditure, represented by a linear second-order difference equation, is able to generate oscillations converging to the equilibrium for a wide range of values of the parameters, and give its analytic solution for all the possible cases. A delayed version proposed in the recent literature, represented by a linear third-order difference equation, is also considered. We show that also this model is able to produce converging oscillations, and give a complete analysis of the stability region of the equilibrium. A new simple nonlinear model is proposed, showing that it keeps oscillatory behavior, although coupled with other dynamics related to global effects. Our analysis confirms that the seminal work of Samuelson and simple modifications of it, may give powerful tools in the study of the business cycles.
Impact of landscape dynamics and intensities on the ecological land of major cities in Ethiopia
Authors: Degefu Mekonnen Amberber , Argaw Mekuria , Feyisa Gudina Legese , Degefa Sileshi 
Published: June 2021

Abstract
Background
Understanding the dependence of ecological land and dynamics of the human-nature-coupled landscape is crucial for urban ecosystem resilience. In this study, we characterized and compared the Spatio-temporal responses of ecological land to urban landscape dynamics in Bahir Dar, Addis Ababa, Adama, and Hawassa cities in Ethiopia for the last three decades (1990–2020). Three sets of Landsat satellite images, field observations, and urban land indexes were used to produce landscape maps and geo-spatial data analysis.

Results
The results showed that in all cities ecological land has had changed intensely during 1990–2020 regarding its quantity, and spatial pattern. Besides, the substantial expansion of built-up ecosystems was manifested at the cost of ecological land. The built-up ecosystem was augmented by 17,341.0 ha (32.16%), 2151.27 ha (19.64%), 2715.21 ha (12.21%), and 2599.65 ha (15.71%) for Addis Ababa, Adama, Bahir Dar, and Hawassa cities respectively from 1990 to 2020 periods. A total of 40.97% of the prolonged built-up area was obtained from urban agricultural land alone. Moreover, urban sprawl is likely to continue, which will be outweighed by the loss of the open space ecosystem. The finding also confirmed the value of land-use intensity (LUI) of Addis Ababa (3.31), Bahir Dar (3.56), Hawassa (4.82), Adama (5.04) was augmented parallel with accelerated growth in the built-up ecosystems. Besides, the Integrated land-use dynamics degree (ILUDD) analysis confirmed that the spatial pattern of ecological land loss significantly consistent with LUI in all cities.

Conclusion
Land-use intensity (LUI) dynamics pattern was followed by urban ecological land to the multi-complex human-dominance ecosystem with a substantial influence on urban greenery and ecosystem services provides. Thus, in all cities, the implementation of effective ecological land management and urban planning policies are required to ensure economic development and ecosystem resilience.
Enhanced mutualistic symbiosis between soil phages and bacteria with elevated chromium-induced environmental stress
Authors: Huang Dan , Yu Pingfeng , Ye Mao , Schwarz Cory , Jiang Xin , Alvarez Pedro J. J. 
Published: June 2021

Abstract
Background
Microbe–virus interactions have broad implications on the composition, function, and evolution of microbiomes. Elucidating the effects of environmental stresses on these interactions is critical to identify the ecological function of viral communities and understand microbiome environmental adaptation. Heavy metal-contaminated soils represent a relevant ecosystem to study the interplay between microbes, viruses, and environmental stressors.

Results
Metagenomic analysis revealed that Cr pollution adversely altered the abundance, diversity, and composition of viral and bacterial communities. Host–phage linkage based on CRISPR indicated that, in soils with high Cr contamination, the abundance of phages associated with heavy metal-tolerant hosts increased, as did the relative abundance of phages with broad host ranges (identified as host–phage linkages across genera), which would facilitate transfection and broader distribution of heavy metal resistance genes in the bacterial community. Examining variations along the pollutant gradient, enhanced mutualistic phage–bacterium interactions were observed in the face of greater environmental stresses. Specifically, the fractions of lysogens in bacterial communities (identified by integrase genes within bacterial genomes and prophage induction assay by mitomycin-C) were positively correlated with Cr contamination levels. Furthermore, viral genomic analysis demonstrated that lysogenic phages under higher Cr-induced stresses carried more auxiliary metabolic genes regulating microbial heavy metal detoxification.

Conclusion
With the intensification of Cr-induced environmental stresses, the composition, replication strategy, and ecological function of the phage community all evolve alongside the bacterial community to adapt to extreme habitats. These result in a transformation of the phage–bacterium interaction from parasitism to mutualism in extreme environments and underscore the influential role of phages in bacterial adaptation to pollution-related stress and in related biogeochemical processes.
Sevoflurane inhibits malignant progression of colorectal cancer via hsa_circ_0000231-mediated miR-622
Authors: Wang Jingpeng , Li Shuyuan , Zhang Gaofeng , Han Huihua 
Published: June 2021

Abstract
Background
Sevoflurane (Sev), a commonly used volatile anesthetic, has been reported to inhibit the process of colorectal cancer (CRC). Circular RNAs (circRNAs) are revealed to participate in the pathogenesis of CRC. This study aims to reveal the mechanism of hsa_circ_0000231 in Sev-mediated CRC progression.

Methods
The expression of hsa_circ_0000231 and microRNA-622 (miR-622) was detected by quantitative real-time polymerase chain reaction (qRT-PCR). Protein level was determined by western blot analysis. Cell proliferation was investigated by 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), cell colony formation and DNA content quantitation assays. Cell apoptosis was detected by Annexin V-fluorescein isothiocyanate and propidium iodide double staining and caspase 3 activity assays. Cell migration and invasion were investigated by wound-healing and transwell invasion assays, respectively. The putative relationship between hsa_circ_0000231 and miR-622 was predicted by circular RNA Interactome online database, and identified by dual-luciferase reporter and RNA immunoprecipitation assays. The impacts of hsa_circ_0000231 on Sev-mediated tumor formation in vivo were presented by in vivo assay.

Results
Hsa_circ_0000231 expression was upregulated, while miR-622 was downregulated in CRC tissues and cells compared with control groups. Sev treatment decreased hsa_circ_0000231 expression, but increased miR-622 expression in CRC cells. Sev treatment suppressed cell proliferation, migration and invasion, and induced cell apoptosis. Hsa_circ_0000231 overexpression restored Sev-mediated CRC progression in vitro. Additionally, hsa_circ_0000231 acted as a sponge of miR-622, and miR-622 inhibitors reversed the impacts of hsa_circ_0000231 silencing on CRC process. Furthermore, Sev treatment inhibited tumor growth by regulating hsa_circ_0000231 in vivo.

Conclusion
Hsa_circ_0000231 attenuated Sev-aroused repression impacts on CRC development by sponging miR-622. This findings may provide an appropriate anesthetic protocol for CRC sufferers undergoing surgery.
Political parties abroad as actors of transnational politics
Authors: van Haute Emilie , Kernalegenn Tudi 
Published: June 2021

AbstractThis paper argues that parties abroad are the actors of a new arena for citizenship and party politics. The proliferation of overseas voting and the development of representative institutions for emigrants has transformed and reinforced the civic and political links between sending-states and their diaspora. This has also created new opportunities for political entrepreneurs and political parties tasked with reaching out to citizens living abroad. Yet research on political parties and on transnationalism has almost never crossed paths. This has created a gap in our knowledge on political parties abroad, demonstrating the timeliness of a special issue on political parties abroad. This paper introduces this special issue and presents an overview of the main theoretical questions and debates addressed in the articles. We emphasize existing gaps in the literature and stress the importance of a better understanding of the growing phenomenon of political parties abroad. We also explain why a comparative approach is necessary to tackle the issue of political parties abroad, offering a theoretically-minded framework. Our summaries of the papers in this special issue highlight how they relate to the more general questions discussed in our introduction.
Few-body perspective on fermionic pairing in one spatial dimension
Authors: Sowiński Tomasz 
Published: June 2021

Abstract
In this perspective we discuss recent theoretical and experimental concepts giving a route to a better understanding of conventional and unconventional pairing mechanisms between opposite-spin fermions arising in one-dimensional mesoscopic systems. With special attention, we focus on the problem of experimental detectability of correlations between particles. We argue that state-of-the-art experiments with few ultracold fermions may finally break an impasse and give pioneering and unquestionable verification of the existence of correlated pairs with non-zero center-of-mass momentum.
Musical Techniques in Balanchine's Jazzy Bach Ballet
Authors: Leaman Kara Yoo 
Published: June 2021

Abstract
The choreography of George Balanchine has long been described as “musical.” By applying music-analytic tools to patterns in dance, this essay analyzes relationships between dance and music in Balanchine's Concerto Barocco, set to J. S. Bach's Concerto for Two Violins (BWV 1043). Choreomusical notation and annotated videos offer readers the chance to sketch-dance, to feel in their own bodies the movements in relation to the music. Balanchine's choreography maps both specific patterns of pitch and rhythm from Bach's score—sometimes synchronized to the music and sometimes displaced temporally—and general patterns of motivic development and metric manipulation. Balanchine's use of funky rhythms resonates with his characteristic on-top-of-the-beat step timing, offbeat visual accentuation, and jazz-dance-inspired movements, attesting to the adoption of both Africanist and Europeanist musical techniques in the formation of an American neoclassical ballet.
Cross-sectional assessment of cardiovascular risk factors in patients with knee osteoarthritis
Authors: Goel Sagar , Kamath Surendra Umesh , Annappa Rajendra , Krishnamurthy Sunil Lakshmipura , Jain Manesh , Thakkar Samarth , Damsas Lulu , Banerjee Sayak , Divakar Prajwal Madapura 
Published: June 2021

Background:Osteoarthritis (OA) and cardiovascular disease (CVD) are prevalent in India. However, there is dearth of literature among Indians studying the relationship between the two. This study was carried out to assess various cardiovascular (CV) risk factors in patients with knee OA with an objective to investigate their association, screening and management. Methods: In total, 225 patients were included in this cross-sectional study. Participants were diagnosed with knee OA on the basis of the Kellgren and Lawrence (K-L) classification of their radiograph. Participants were also assessed for CV risk factors (age, body mass index, systolic blood pressure, diabetes mellitus, total cholesterol, high-density lipoprotein, smoking) with the help of the Joint British Society QRisk3 calculator (JBS3), which gave three variables: JBS3 risk score, heart age, and life expectancy. Chi Square, Fishers exact test and one-way ANOVA tests were used to compare the categorical and quantitative variables, respectively. Pearson’s correlation coefficient was used to assess the relationship between CV risk factors and knee OA.Results: Patients with severe knee OA had a statistically significantly higher prevalence of CV risk factors (p<0.05). Grade 4 knee OA patients were found to have a mean JBS3 risk of 38%, heart age of 82 years and life expectancy of 77 years as compared to grade 2 patients who had a mean JBS3 risk of 11%, heart age of 63 years and life expectancy of 82 years. Conclusions: Our study concluded that there is a strong positive correlation between knee OA and CVD, with CV risk score being directly proportional to the severity of OA. JBS3 is a comprehensive risk score calculator as well as a screening tool, which produces three more comprehensive variables, namely 10-years risk of developing CVD, physiological heart age and life expectancy.