bluuebunny/crossref_metadata_2025_split huggingface dataset:
Fuscimiditide: a RiPP with Ω-Ester and Aspartimide Post-translational Modifications
Authors: Elashal Hader E. , Koos Joseph D. , Cheung-Lee Wai Ling , Choi Brian , Cao Li , Richardson Michelle A. , White Heather L. , Link A. James 
Published: May 2021

AbstractMicroviridins and other ω−ester linked peptides (OEPs) are characterized by sidechain-sidechain linkages installed by ATP-grasp enzymes. Here we describe the discovery of a new family of OEPs, the gene clusters of which also encode an O-methyltransferase with homology to the protein repair catalyst protein L-isoaspartyl methyltransferase (PIMT). We produced the first example of this new ribosomally synthesized and post-translationally modified peptide (RiPP), fuscimiditide, via heterologous expression. NMR analysis of fuscimiditide revealed that the peptide contains two ester crosslinks forming a stem-loop macrocycle. Furthermore, an unusually stable aspartimide moiety is found within the loop macrocycle. We have also fully reconstituted fuscimiditide biosynthesis in vitro establishing that ester formation catalyzed by the ATP-grasp enzyme is an obligate, rate-limiting first biosynthetic step. Aspartimide formation from aspartate is catalyzed by the PIMT homolog in the second step. The aspartimide moiety embedded in fuscimiditide hydrolyzes regioselectively to isoaspartate (isoAsp). Surprisingly, this isoAsp-containing protein is also a substrate for the PIMT homolog, thus driving any hydrolysis products back to the aspartimide form. Whereas aspartimide is often considered a nuisance product in protein formulations, our data here suggest that some RiPPs have aspartimide residues intentionally installed via enzymatic activity.Abstract Figure
Global Patterns in Culturable Soil Yeast Diversity
Authors: Samarasinghe Himeshi , Lu Yi , Aljohani Renad , Al-Amad Ahmad , Yoell Heather , Xu Jianping 
Published: May 2021

SummaryYeasts, broadly defined as unicellular fungi, fulfill essential roles in soil ecosystems as decomposers and nutrition sources for fellow soil-dwellers. Broad-scale investigations of soil yeasts pose a methodological challenge as metagenomics are of limited use on this group of fungi. Here we characterize global soil yeast diversity using fungal DNA barcoding on 1473 yeasts cultured from 3826 soil samples obtained from nine countries in six continents. We identify mean annual precipitation and international air travel as two significant predictors of soil yeast community structure and composition worldwide. Anthropogenic influences on soil yeast communities, directly via travel and indirectly via altered rainfall patterns resulting from climate change, are concerning as we found common infectious yeasts frequently distributed in soil in several countries. Our discovery of 41 putative novel species highlights the need to revise the current estimate of ~1500 recognized yeast species. Our findings demonstrate the continued need for culture-based studies to advance our knowledge of environmental yeast diversity.
Mechanisms underlying microglial colonization of developing neural retina in zebrafish
Authors: Ranawat Nishtha , Masai Ichiro 
Published: May 2021

AbstractMicroglia are brain-resident macrophages that function as the first line of defense in brain. Embryonic microglial precursors originate in peripheral mesoderm and migrate into the brain during development. However, the mechanism by which they colonize the brain is incompletely understood. The retina is one of the first brain regions to accommodate microglia. In zebrafish, embryonic microglial precursors use intraocular hyaloid blood vessels as a pathway to migrate into the optic cup via the choroid fissure. Once retinal progenitor cells exit the cell cycle, microglial precursors associated with hyaloid blood vessels start to infiltrate the retina preferentially through neurogenic regions, suggesting that colonization of retinal tissue depends upon the neurogenic state. Along with blood vessels and retinal neurogenesis, IL34 also participates in microglial precursor colonization of the retina. Altogether, CSF receptor signaling, blood vessels, and neuronal differentiation function as cues to create an essential path for microglial migration into developing retina.
Changes of chromosomal architecture before establishment of chromosome territories revealed by recurrence plot reconstruction
Authors: Kitanishi Yuki , Sugishita Hiroki , Gotoh Yukiko , Hirata Yoshito 
Published: May 2021

AbstractThe chromatin conformation capture-related methods such as Hi-C have improved our understanding of nuclear architecture and organization in recent years. However, reconstruction of nuclear architecture from single-cell Hi-C (scHi-C) data is challenging due to limited information of DNA contacts obtained from a single cell. We have previously developed the Recurrence Plot-Based Reconstruction (RPR) method for reconstructing three dimensional (3D) genomic structure from Hi-C data of single haploid cells (1) and diploid cells (2). This algorithm is based on a recurrence plot, a tool of nonlinear time-series analysis for visualizing patterns within a time series (3, 4), and enables the reconstruction of a unique 3D chromosome architecture even from low-coverage DNA contact information. Here we used the RPR method to analyzing published scHi-C data of diploid cells derived from early-stage F1 hybrid embryos (5) as a proof-of-concept for understanding of global developmental changes in chromosomal architecture of early stage embryos. We found that paternal and maternal chromosomes become gradually intermingled from 1 cell to 64 cell stage, and that discrete chromosome territories are largely established between 8 cell and 64 cell stages. We also observed Rabl-like polarization of chromosomes from the 2-to 8-cell stages, but this polarization becomes mostly dissolved by the 64-cell stage. Rabl-like chromosome polarization precedes rod-like extension and parallel alignment of chromosomes, implicating the role of Rabl-like polarization in effective mixing of chromosomes before establishing chromosome territories. We also found cell-to-cell variability in chromatin configuration. A combination of scHi-C and RPR analyses can depict features of the 3D chromatin architecture of individual cells at different developmental stages during early embryogenesis.
In vitro models of the human esophagus reveal ancestrally diverse response to injury
Authors: Ferrer-Torres Daysha , Wu Joshua H. , Zhang Charles J. , Hammer Max A. , Dame Michael , Wu Angeline , Holloway Emily M. , Karpoff Kateryna , McCarthy Caroline L. , Bohm Margaret S , Huang Sha , Tsai Yu-Hwai , Hogan Simon P. , Turgeon Danielle Kim , Lin Jules , Higgins Peter D.R. , Sexton Jonathan , Spence Jason R. 
Published: May 2021

SummaryEuropean Americans (EA) are more susceptible to esophageal tissue damage and inflammation when exposed to gastric acid and bile acid reflux and have a higher incidence of esophageal adenocarcinoma when compared to African Americans (AA). Population studies have implicated specific genes for these differences; however, the underlying cause for these differences is not well understood. We describe a robust long-term culture system to grow primary human esophagus in vitro, use single cell RNA sequencing to compare primary human biopsies to their in vitro counterparts, identify known and new molecular markers of basal cell types, and demonstrate that in vivo cellular heterogeneity is maintained in vitro. We further developed an ancestrally diverse biobank and a high-content, image based, screening assay to interrogate bile-acid injury response. These results demonstrated that AA esophageal cells responded significantly differently than EA-derived cells, mirroring clinical findings, having important implications for addressing disparities in early drug development pipelines.
Gender-biased office culture in Croatian PR industry: why feminine sectors practice masculine patterns?
Authors: Polić Mirela , Holy Mirela 
Published: May 2021

PurposeThis paper aimed to research attitudes of women working in the Croatian public relations (PR) industry regarding office culture (networking, banter, dress codes, etc.).Design/methodology/approachThis paper uses Bourdieu's habitus theory and analyses the experiences of women working in the Croatian PR industry with a focus on office culture. The study is based on 21 in-depth interviews with women working in the Croatian PR industry. Thematic analysis has been used to analyse data.FindingsAlthough female employees (76.84%) dominate the PR industry in Croatia, the so-called masculine patterns still prevail in the PR sector. Results show that women are often exposed to gender discrimination but at the same time, they also perpetuate gender-based prejudices.Practical implicationsEmployees in the PR industry should consider working on their own gender stereotypes that impact their patterns of behaviour. Relinquishment of the ideas of patriarchal essentialism would consequently change career progression opportunities, and it would particularly improve networking among women, which could lead to career advancement opportunities.Social implicationsStructural changes are needed in society to avoid women perpetuating inequality through masculine behaviour and unrealistic expectations that many women cannot meet.OriginalityTo the best of the author's knowledge, this paper is the first paper that explores gender-biased office culture in the Croatian PR industry. In doing so, the paper also applies Bourdieu's habitus theory, thus contributing to studying cultural masculinities from a Croatian perspective. The paper also introduces the concept of gender-biased behaviour.
Applications of object detection in modular construction based on a comparative evaluation of deep learning algorithms
Authors: Liu Chang , M.E. Sepasgozar Samad , Shirowzhan Sara , Mohammadi Gelareh 
Published: May 2021

Purpose
The practice of artificial intelligence (AI) is increasingly being promoted by technology developers. However, its adoption rate is still reported as low in the construction industry due to a lack of expertise and the limited reliable applications for AI technology. Hence, this paper aims to present the detailed outcome of experimentations evaluating the applicability and the performance of AI object detection algorithms for construction modular object detection.

Design/methodology/approach
This paper provides a thorough evaluation of two deep learning algorithms for object detection, including the faster region-based convolutional neural network (faster RCNN) and single shot multi-box detector (SSD). Two types of metrics are also presented; first, the average recall and mean average precision by image pixels; second, the recall and precision by counting. To conduct the experiments using the selected algorithms, four infrastructure and building construction sites are chosen to collect the required data, including a total of 990 images of three different but common modular objects, including modular panels, safety barricades and site fences.

Findings
The results of the comprehensive evaluation of the algorithms show that the performance of faster RCNN and SSD depends on the context that detection occurs. Indeed, surrounding objects and the backgrounds of the objects affect the level of accuracy obtained from the AI analysis and may particularly effect precision and recall. The analysis of loss lines shows that the loss lines for selected objects depend on both their geometry and the image background. The results on selected objects show that faster RCNN offers higher accuracy than SSD for detection of selected objects.

Research limitations/implications
The results show that modular object detection is crucial in construction for the achievement of the required information for project quality and safety objectives. The detection process can significantly improve monitoring object installation progress in an accurate and machine-based manner avoiding human errors. The results of this paper are limited to three construction sites, but future investigations can cover more tasks or objects from different construction sites in a fully automated manner.

Originality/value
This paper’s originality lies in offering new AI applications in modular construction, using a large first-hand data set collected from three construction sites. Furthermore, the paper presents the scientific evaluation results of implementing recent object detection algorithms across a set of extended metrics using the original training and validation data sets to improve the generalisability of the experimentation. This paper also provides the practitioners and scholars with a workflow on AI applications in the modular context and the first-hand referencing data.
Kingfisher: Ultra Max re-positioning hurdle
Authors: Rishi Bikramjit , Kacker Archit 
Published: May 2021

Learning outcomes
To appraise the product positioning in an emerging market. To recognize and discuss a positioning plan for a new product. To apply, describe the Kapferer brand identity prism along with different competition levels for Kingfisher Ultra Max. To deliberate the marketing mix for improving the sales of Kingfisher Ultra Max.

Case overview/synopsis
United Breweries Limited (UBL) was part of UB Group, which was a business conglomerate. United Breweries Holdings Limited or UB Group was headquartered at UB City, Bangalore, India. It dealt in many businesses, out of which UBL was one of them. Kingfisher Ultra Max was Kingfisher’s newest addition to the super-premium strong beer segment. It was a larger-based beer with 8% alcohol by volume content and was stronger in terms of alcohol content than Kingfisher Ultra, which was also from the super-premium segment. This brands positioning was such that it targeted the premium segment. The top management was considering a change in positioning for their Ultra Max brand. A research report submitted by a premier business school also recommended a change in positioning. The officials in the meeting are contemplating the two options for the shift in positioning; one is to make the change of positioning across India and the other is to make the change specific to some states.

Complexity academic level
The case is targeted at students of post-graduation and under-graduation programs in business administration, specializing in marketing management, brand management or marketing strategy. Also, the case study can be included as part of courses related to strategic management and competitive analysis.

Supplementary materials
Teaching Notes are available for educators only. Please contact your library to gain login details or email support@emeraldinsight.com to request teaching notes.

Subject code
CSS 8: Marketing.

Supplementary materials
Teaching Notes are available for educators only.
Sana enterprises: location selection
Authors: Shaikh Abdul Rehman , Qazi Asad Ali 
Published: May 2021

Learning outcomes
To understand the strategic importance of location selection within the organization. To analyze the constraints in decision-making for selection of location. To analyze the alternate options for a location selection. To understand the usage of the factor rating method.

Case overview/synopsis
Due to a countrywide anti-encroachment drive, Mr Mughal loses his shop. He had just received a notice that his shop including those of others near him was established on one of the amenity plots. The structure was declared as illegal and was to be demolished in 24 h. He had to vacate the shop and his display center to avoid the loss of his items. He along with other shop owners approached to Supreme Court of Pakistan (SCP) to stop this demolishing act and to prove that these shops belonged to them for decades and that they had already paid the price of shops at that time. However, the SCP rejected their appeal straightforward and the anti-encroachment drive was carried out. Now, Mr Mughal had to find out an alternate location to establish his display center and to resume his business operations.

Complexity academic level
Undergraduate.

Supplementary materials
Teaching Notes are available for educators only.

Subject code
CSS 9: Operations and logistics.
Sushruth Ayurved Industry: strategies for growth
Authors: Kidiyoor Gururaj , Yatgiri Prashant V. 
Published: May 2021

Learning outcomes
Understand the dynamics of the diabetes supplement market and carry out an industry analysis using Porter’s five force analysis. Understand the challenges faced by a small entrepreneur in setting up distribution channels and examine the channel powers that come into play in the given context. Discuss the merits and demerits of traditional vs online channels. Understand the factors that are important to succeed in a highly competitive diabetes supplement industry (this would include aspects such as value sought by end customer, business-to-business [B2B] buyers, expertise required to handle B2B customers and also the price and salesforce reward approaches). Enumerate the merits and demerits of individual product branding vs an umbrella brand for a company selling over-the-counter (OTC) drugs online. Understand the various considerations for export marketing for OTC drugs.

Case overview/synopsis
Sushruth Ayurved Industry (SAI) is a proprietorship firm owned by Girish Banvi who always dreamt of being an entrepreneur. He had set up SAI to produce diabetes supplement by the name “Sugar Knocker” to give wings to his dreams. Notwithstanding competition from corporate players and demands from health-care practitioners, he had to abandon his traditional route to selling his product and open his eyes to online marketing. He believed it could provide him the perfect medium to reach his prospects directly without any middlemen within a cost-effective budget. SAI registered revenue of INR 24m per year, completely attributable to online sales. With a firm footing in the online space, Girish was now exploring physical marketing to expand his audience reach in the B2B market and also add new products to his portfolio. He was also worried about the low capacity utilization of his manufacturing unit, which stood at 20%. With only 30% of the 40 formulations used, there was much scope for expansion. With his plant capacity underused, the time was ripe for Girish to trace his footsteps from where he had begun in the first place.

Complexity academic level
This case can be used in marketing management course under the marketing strategy module. It can also find use in the elective course on marketing channels, and in sectoral programs such as health-care management or MBA in health care. This case can also be used in the health-care products marketing course.

Supplementary materials
Teaching notes are available for educators only.

Subject code
CSS 8: Marketing.
Determinants of radical and incremental innovation: the influence of transformational leadership, knowledge sharing and knowledge-centered culture
Authors: Gui Linnan , Lei Hui , Le Phong Ba 
Published: May 2021

PurposeThe purpose of this study is to clarify the effects of transformational leadership (TL) on radical and incremental innovation through the mediating roles of knowledge sharing (KS) behaviors. This study also attempts to bring deeper insight of the correlation among the latent factors by examining how knowledge-centered culture (KCC) moderates the effects of TL on KS behaviors and innovation capability.Design/methodology/approachThis study conducts a questionnaire survey and Structural Equation Modeling to test the proposal research model based on a sample of 321 participants in 85 service and manufacturing firms.FindingsThe results revealed that KS behaviors significantly mediate the relationship between TL and two types of innovation. Specifically, knowledge collecting has more significant effect on incremental innovation compared with influence of knowledge donating. By contrast, knowledge donating has a greater effect on radical innovation compared with effect of knowledge collecting. The paper also highlights the important role of KCC in enhancing the effects of TL on KS activities and innovation capabilities.Research limitations/implicationsThe study has highlighted the important role of KCC as it positively moderates the influences of TL on KS and innovation capability.Practical implicationsThe study provides evidence that TL style is the main driving force of KS processes. Managers should prioritize practicing this leadership style to improve employees' KS behaviors and firm's innovation capability.Originality/valueThe study significantly fills the gaps in the literature that emphasizes how KS behaviors induce different impacts on specific aspects of innovation capability. By exploring the moderator of KCC, the paper significantly contributes to advancing the understanding of how transformational leaders foster employees' KS behaviors for improving radical and incremental innovation.
A complexity management approach to servitization: the role of digital platforms
Authors: Eloranta Ville , Ardolino Marco , Saccani Nicola 
Published: May 2021

PurposeThis study aims to enhance the theoretical foundations of servitization research by establishing a theoretical connection with complexity management. The authors develop a conceptual framework to describe complexity management mechanisms in servitization and digital platforms' specific role in allowing synergies between complexity reduction and absorption mechanisms.Design/methodology/approachA theory adaptation approach is used. Theory adaptation introduces new perspectives and conceptualization to the domain theory (servitization, with a focus on the role of digital platforms) by informing it with a method theory (complexity management).FindingsThis study provides four key contributions to the servitization literature: (1) connecting the servitization and complexity-management terminologies, (2) identifying and classifying complexity-management mechanisms in servitization, (3) conceptualizing digital platforms' role in servitization complexity management and (4) recognizing digital platforms' complexity-management synergies.Originality/valueThis study highlights that by using digital platforms in servitization and understanding the platform approach more thoroughly, companies can gain new capabilities and opportunities to manage and leverage complexity.