bluuebunny/crossref_metadata_2025_split huggingface dataset:
Enhancing Collagen Biosynthesis in Mammalian Cells Through Hypoxia-Mimetic Prolyl Hydroxylase Inhibition
Authors: Shahar Bar , Kilimnik Itay , Lifshits Lucia Adriana , Netti Francesca , Sova Marina , Rosin-Grunewald Dalia , Gal Maayan , Adler-Abramovich Lihi 
Published: December 2024

AbstractCollagen, the most abundant protein in the extracellular matrix of mammalian cells, is extensively needed in various biotechnological and therapeutic applications, such as tissue engineering and regeneration, cosmetics, and cultivated meat. Despite the increasing demand for natural collagen from non-animal sources, it is mainly produced from animal connective tissues. Recent research has highlighted that under hypoxia, the activation of the hypoxia-inducible factor (HIF) leads to enhanced collagen type I biosynthesis. However, under normal oxygen conditions, HIF activity is downregulated by the HIF-prolyl hydroxylase (PHD) enzyme. We, therefore, hypothesized that inhibiting PHD could elevate HIF transcriptional activity and enhance collagen biosynthesis under normoxia. Our study demonstrates that inhibiting PHD using exogenous small molecules boosts HIF activity and upregulates the key enzymes, collagen prolyl 4-hydroxylases and lysyl hydroxylases, resulting in up to 29-fold increase in collagen type I in embryonic mouse fibroblast NIH/3T3 cells. These findings suggest that targeting PHD can effectively enhance collagen production in mammalian cells. Therefore, modulating key protein signaling pathways presents a promising strategy for enhancing the production of high-yield natural collagen.
Inhibition of Mitochondrial Fission Protein Drp1 Ameliorates Myopathy in the D2-mdx Model of Duchenne Muscular Dystrophy
Authors: Rosen H. Grace , Berger Nicolas J. , Hodge Shantel N. , Fujishiro Atsutaro , Lourie Jared , Kapadia Vrusti , Linden Melissa A. , Jee Eunbin , Kim Jonghan , Kim Yuho , Zou Kai 
Published: December 2024

ABSTRACTAlthough current treatments for Duchenne Muscular Dystrophy (DMD) have proven to be effective in delaying myopathy, there remains a strong need to identify novel targets to develop additional therapies. Mitochondrial dysfunction is an early pathological feature of DMD. A fine balance of mitochondrial dynamics (fission and fusion) is crucial to maintain mitochondrial function and skeletal muscle health. Excessive activation of Dynamin-Related Protein 1 (Drp1)-mediated mitochondrial fission was reported in animal models of DMD. However, whether Drp1-mediated mitochondrial fission is a viable target for treating myopathy in DMD remains unknown. Here, we treated a D2-mdx model of DMD (9-10 weeks old) with Mdivi-1, a selective Drp1 inhibitor, every other day (i.p. injection) for 5 weeks. We demonstrated that Mdivi-1 effectively improved skeletal muscle strength and reduced serum creatine kinase concentration. Mdivi-1 treatment also effectively inhibited mitochondrial fission regulatory protein markers, Drp1(Ser616) phosphorylation and Fis1 in skeletal muscles from D2-mdx mice, which resulted in reduced content of damaged and fragmented mitochondria. Furthermore, Mdivi-1 treatment attenuated lipid peroxidation product, 4-HNE, in skeletal muscle from D2-mdx mice, which was inversely correlated with muscle grip strength. Finally, we revealed that Mdivi-1 treatment downregulated Alpha 1 Type I Collagen (Col1a1) protein expression, a marker of fibrosis, and Interleukin-6 (IL-6) mRNA expression, a marker of inflammation. In summary, these results demonstrate that inhibition of Drp1-mediated mitochondrial fission by Mdivi-1 is effective in improving muscle strength and alleviating muscle damage in D2-mdx mice. These improvements are associated with improved skeletal muscle mitochondrial integrity, leading to attenuated lipid peroxidation.
Modulation of hippocampal sharp-wave ripples by behavioral states and body movements in head-fixed rodents
Authors: Rios Alain , Minori Usui , Isomura Yoshikazu 
Published: December 2024

AbstractHippocampal sharp-wave ripples (SWRs) are critical events implicated in memory consolidation, planning, and the reactivation of recent experiences. Under freely moving conditions, a well-established dichotomy exists: hippocampal networks predominantly generate theta oscillations during periods of reward pursuit (preparatory behaviors) and exhibit pronounced SWR activity once the reward is achieved (consummatory behaviors). Here, we examined how SWRs are modulated by reward delivery and subtle movements in head-fixed rats. Contrary to the canonical view established in freely moving settings, we found that the dominant and more enduring effect was a sustained suppression of SWR activity immediately following water delivery. Moreover, even minor, localized movements (such as whisking or body adjustments) decreased SWR occurrence, demonstrating that hippocampal ripple generation is highly sensitive to motor engagement, irrespective of reward timing. Such movement-induced suppression of ripples persisted during both sleep-like states and quiet wakefulness, suggesting that while large-scale brain states modulate the overall likelihood of SWR generation, local motor-related influences exert a state-independent inhibitory effect on hippocampal ripples. Our results show that SWR modulation by behavioral states and body movements is more context-dependent than previously appreciated.
Population genomics reveals an ancient origin of heartworms in canids
Authors: Power Rosemonde I. , Abdullah Swaid , Walden Heather S. , Verocai Guilherme G. , Sanders Tiana L. , Luksovsky Joe L. , Moorhead Andrew R. , Dzimianski Michael T. , Foster Jeremy M. , Michalski Michelle L. , Rojas Alicia , Chacón Samuel C. , Deak Georgiana , Mihalca Andrei D. , Danesi Patrizia , Papadopoulos Elias , Taweethavonsawat Piyanan , Bui Dung Thi , Ngoc Anh Do , Sharma Reuben S.K. , Ho Simon Y.W. , Doyle Stephen R. , Šlapeta Jan 
Published: December 2024

AbstractHeartworms (Dirofilaria immitis) are parasitic nematodes that cause significant cardiopulmonary-associated morbidity and mortality in canids worldwide. The global dissemination of heartworms is believed to have occurred alongside the dispersal of domesticated dogs. To test this theory, we performed the largest population genetics study of heartworms to date, based on whole-genome sequencing of 127 modern adult individuals collected from mammalian carnivore hosts across four continents. Population structure and demographic analyses of the nuclear genome reveal distinct genetic differences between heartworms from different continents, indicating a deeper ancient origin and dispersal in canid hosts than previously recognised. Using admixture analyses, we find an Asian origin for Australian heartworms consistent with the arrival of dingoes thousands of years ago. Finally, the genetic relatedness between European and Central American heartworms suggests that modern dispersal, likely associated with human colonisation of the Americas by Europeans, occurred with domesticated dogs. Our findings shed light on the global population dynamics and evolutionary history of heartworms, which can aid future surveillance and control efforts for this important veterinary parasite.
Network vulnerability of cattle movement in Minas Gerais, Brazil, from 2013 to 2022
Authors: Costa Anna Cecília Trolesi Reis Borges , Savini Lara , de Oliveira Luciana Faria , Lage Andrey Pereira , Dorneles Elaine Maria Seles , Candeloro Luca 
Published: December 2024

AbstractThe aim of this study was to analyze the network vulnerability of cattle movements from 2013 to 2022, in Minas Gerais, Brazil and to identify the spatial spreaders into the network to improve infectious disease control programs by targeted risk-based surveillance and intervention. The vulnerability was calculated considering the graphs diameter and the spatial spreaders with a threshold distance of 300 km, for incoming (IN) and outgoing (OUT) movements. Additionally, a risk-based analysis was performed in the more vulnerable region. The results showed Triângulo Mineiro / Alto Paranaíba with higher vulnerability and many IN spatial spreaders, as well as Vale do Mucurí region with many OUT spatial spreaders. The risk-based analysis revealed betweenness and out degree as the most effective measures to be considered for intervention. Therefore, the vulnerability analysis and the spatial spreader were observed as great tools for risk-based interventions and surveillance. Furthermore, Triângulo Mineiro / Alto Paranaíba and Vale do Mucuri regions were important regions, considering restriction of animal infectious disease spread in Minas Gerais, Brazil.
Playful TikTok videos: investigating the role of mental imagery in customers’ social media sharing and destination attitude
Authors: Yuan Yanzhe , Surachartkumtonkun Jiraporn , Shao Wei 
Published: December 2024

PurposeThis research explores how playful TikTok video content influences customers’ attitudes towards travel destinations and their intention to share. It examines the role of mental imagery in shaping these responses and investigates how perceived novelty moderates the relationship between playfulness and mental imagery.Design/methodology/approachWe conducted a scenario-based online survey using an actual video from a real Chinese influencer, with 701 tourists participating. In the second study, we used an experimental research design and created a video specifically for the experiment, where we manipulated the playfulness of the content, involving 402 participants.FindingsThe results show that the playfulness of TikTok videos enhances mental imagery, which mediates the positive effect on customers’ attitudes and sharing intentions. Novelty further strengthens the impact of playfulness on mental imagery, highlighting the importance of both factors in destination marketing.Research limitations/implicationsData were collected in mainland China, where the travel short video content was shared by the blogger. Future research should validate these findings with travellers from different countries and destinations, expanding the understanding of consumer engagement on social media for destination marketing.Practical implicationsThis research has significant implications for creating destination marketing strategies on TikTok. Marketing managers and influencers should prioritise crafting playful video content while considering the unique appeal and novelty of the travel destination. This approach is crucial for fostering active customer engagement online.Originality/valueThis research advances our understanding of how playfulness and novelty in TikTok videos influence mental imagery, impacting consumer attitudes and behaviour. It contributes to destination marketing by highlighting the mediating role of mental imagery and the moderating effect of novelty in shaping customer responses and sharing intentions.
Global insights on public sector accounting reforms: a comprehensive study on the adoption, implementation, and impact of IFRS and IPSAS
Authors: Masoud Najeb 
Published: December 2024

Purpose
This paper aims to investigate the adoption of International Financial Reporting Standards (IFRS) and International Public Sector Accounting Standards (IPSAS) and their impact on financial transparency, public sector accountability and economic performance across 173 jurisdictions from 2000 to 2022.

Design/methodology/approach
A robust quantitative approach using econometric panel data analysis with fixed- and random-effects models evaluates the effects of IFRS and IPSAS adoption. This study compares 69 adopting countries with 104 non-adopting or partially adopting nations, examining cross-country and temporal dynamics.

Findings
This paper provides compelling evidence that adopting IFRS and IPSAS significantly improves financial transparency and public sector accountability. Countries adopting these standards, mainly developed economies, experience increased foreign direct investment and improved sovereign credit ratings. The impact is more pronounced in nations with solid institutional frameworks while developing countries benefit from enhanced governance and reduced corruption, though to a lesser extent. These findings contribute to the literature by demonstrating how international accounting standards enhance governance quality and economic performance.

Research limitations/implications
The study’s limitations include the availability and reliability of financial data in jurisdictions with weaker institutional systems. Future research should include longitudinal case studies and stakeholder analyses to explore the nuanced effects of IFRS and IPSAS adoption in different regions.

Practical implications
The results emphasise the need for tailored implementation strategies, especially for developing countries. Strengthening institutional frameworks, investing in capacity-building programmes and enhancing financial literacy are essential to maximising the benefits of IFRS and IPSAS adoption. Policymakers can leverage these standards to improve transparency, attract investment and promote economic stability.

Social implications
By improving financial transparency and accountability, IFRS and IPSAS foster better governance, reduce corruption and promote more equitable resource distribution. These reforms have a particularly positive impact on developing countries, enhancing public trust, supporting social equity and encouraging sustainable economic development.

Originality/value
This paper provides a unique contribution to the discourse on global financial standards. It offers a comprehensive quantitative analysis of IFRS and IPSAS adoption over 23 years and valuable insights for policymakers and financial regulators seeking to strengthen governance and promote global economic integration.
An explicit peridynamics model for hydraulic fracturing in porous media
Authors: Sun Yanan , Zhang Guoyin , Edwards Michael G. , Li Chenfeng 
Published: December 2024

PurposeThe purpose of this paper is to systematically investigate the hydraulic fracture branching phenomena in porous media under different loading conditions and the stepwise phenomenon. The effect of the pore pressure in hydraulic fracturing branching is studied, and more evidence for the stepwise phenomenon with the peridynamics approach is provided.Design/methodology/approachA fully coupled fluid-filled explicit peridynamics model is developed to simulate the complex evolution of crack branching and stepwise phenomena in saturated porous media. Based on the peridynamics theory, an explicit time integration scheme is used to solve the coupled equation system including rock deformation, fluid flow and fracture propagation. Using the proposed model, a series of peridynamic computational tests are performed to examine two common kinds of phenomena observed in hydraulic fracturing: the crack branching phenomenon and the stepwise phenomenon.FindingsFor crack branching phenomenon, the results obtained indicate that sufficient loading is required in order to initiate the crack branching process. Compared with the stress applied on crack surfaces condition, crack branching is more easily induced with the stress applied on boundaries. In addition, for the fluid-driven crack (stress applied on crack surfaces), the existence of pore pressure will depress the growth and branching of the crack. For stepwise phenomena, the results obtained indicate that the peridynamics is a promising tool to study the stepwise phenomenon. The stepwise phenomenon is more distinct under mechanical loading conditions due to the solid behaviour. A sudden jump or crack extension will happen when enough energy is accumulated in the hydraulic fracturing system.Research limitations/implicationsIn this study, the explicit method is used, which means it is conditionally stable, and the critical time step needs to be decided. The reason to use the explicit method is for the study purpose; the explicit method is faster and has no need for matrix inversions.Originality/valueThis study helps to understand the effect of the pore pressure in hydraulic fracturing branching and provides more evidence for the stepwise phenomenon with peridynamics.
Higher education management in developing countries: a bibliometric review
Authors: Olaleye Sunday Adewale , Gbadegeshin Saheed A. , Balogun Oluwafemi Samson , Agbo Friday Joseph , Mogaji Emmanuel 
Published: December 2024

Purpose
This study aims to investigate scholarly works on higher education management from emerging economies. It investigates how higher education management has evolved in emerging economies.

Design/methodology/approach
This study is quantitative, and the authors retrieved the metadata from the Web of Science, an extensive pool of interdisciplinary peer-reviewed literary databases for bibliometric analysis.

Findings
The results show the strengths, weaknesses and prospects of higher education institutions (HEIs) in developing countries based on the scholarly output in various journals.

Research limitations/implications
Though the study contributed to the knowledge and expansion of literature in HEIs research, it was only limited to the Web of Science database.

Practical implications
Policymakers and practitioners who want to improve the efficiency and viability of HEIs in developing countries can find a helpful guide in the form of a framework for higher education management in developing countries.

Social implications
It is important to note that the bibliometric impact of higher education management in developing countries might vary depending on various factors, including the quality and relevance of the research, the level of funding and resources available for research and the level of international collaboration, among others.

Originality/value
This study provides a comprehensive overview of the research landscape in higher education management in developing countries by identifying the most influential authors, institutions and countries and the key research topics and trends. This information can be helpful for researchers, policymakers and practitioners who seek to understand the state of research in the field and identify gaps in knowledge.
How emerging-market brands can overcome a weak country image
Authors: Raji Mustafa , Magnusson Peter , Martirosyan Yuri 
Published: December 2024

PurposeThis study offers a comprehensive analysis of the relative effectiveness of different strategies that emerging-market brands can use to mitigate a weak country image.Design/methodology/approachThis study employs discrete choice conjoint analysis to evaluate the relative effectiveness of store image, warranty duration, third-party certification and corporate engagement on US consumers’ perceptions of products from China and Cambodia. China, representing an advanced emerging market, and Cambodia, representing a less advanced market, were selected to provide a diverse context for comparison. The study examines electric scooters (relatively more hedonic) and refrigerators (relatively more utilitarian) to test the robustness of the findings across different product categories.FindingsThe study demonstrates that retail store image is the most influential factor in mitigating negative COO effects. An extensive warranty emerges as the second most effective mitigation strategy, followed closely by third-party certification. In contrast, corporate social engagement is found to be the least effective strategy across all studies. The study further explores moderating influences on these preferences. The findings are largely robust, with only minimal effects of age, gender, product home country and product type on the relative effectiveness of the identified mitigation strategies.Practical implicationsThe findings offer valuable insights for managers of emerging-market brands, suggesting a prioritization of marketing efforts.Originality/valueThe study enhances the theoretical understanding of COO effects, presenting a nuanced view of how various strategies can be deployed to mitigate negative perceptions of brands associated with emerging markets.
Deep learning application for marketing engagement – its thematic evolution
Authors: Yu Billy T.W. , Liu Stella T.X. 
Published: December 2024

PurposeThis analysis examines the evolving role of deep learning in engagement marketing research. It tries to address a critical knowledge gap despite the rapid growth of artificial intelligence (AI) applications in this field.Design/methodology/approachUsing bibliometric techniques, this study analyzes Scopus data to investigate the evolution of engagement marketing research influenced by technology. Overlapping maps, evolution maps and strategic diagrams reveal key trends and intellectual structures within this dynamic field.FindingsOur analysis reveals key trends in deep learning applications, like focuses on language-interaction, interactivity-privacy and human-focus satisfaction. While results show the contribution in foundational works like linguistics, algorithms and interactive marketing, they also raise concerns about the algorithmic bias, privacy violations and etc.Research limitations/implicationsWhile Scopus data offers valuable insights, our analysis acknowledges its limitations on publication language. Future research should treasure foundational works and historical context for comprehensive understandings. Additionally, addressing emerging challenges such as negative customer experiences and fairness is crucial for future studies.Originality/valueThis review provides a comprehensive perspective on deep learning applications on engagement marketing research in the context of interactive marketing. We present trends and thematic structures with practical implications for scholars and practitioners. It presents a fuller intellectual landscape and suggests that future research directions shall prioritize a human-centered approach to AI implementation, ultimately fostering genuine customer connections.
Publication stress amongst scholars and faculties: a concern of mental health
Authors: S. Raksithaa 
Published: December 2024

Purpose
The purpose of this paper is to explore the impact of the seemingly entrenched culture of “publish or perish” on academics’ and lecturers’ mental health in academia. From an autoethnographic perspective, personal experiences of stress, anxiety and burnout are articulated and considered in terms of broader system issues within academia.

Design/methodology/approach
Using personal reflections on publication pressure and combining that with the broader existing literature on mental health in academia, this paper, like the ones mentioned above, has been written with autoethnography as the research mode. Autoethnography is a research method that allows for profoundly exploring personal experiences but frames them in a broader academic context, thereby allowing for a qualitative analysis of academics’ mental health challenges.

Findings
The pressure to publish in high-impact journals puts a person under a level of mental health stress that includes feeling anxious, feeling like an impostor and suffering from burnout. Therefore, this very unfitting competitive environment requires institutional support and strategies to mitigate the stress associated with publication.

Originality/value
This paper offers an autoethnographic view of the mental health difficulties in academia, providing a firsthand account of the emotional toll of academic publishing. This paper fleshes out the burgeoning discourse surrounding mental health within higher education by connecting personal experiences with systemic issues, pointing to changes in culture and structure.