bluuebunny/crossref_metadata_2025_split huggingface dataset:
Adaptive plasticity of skeletal muscle energetics in hibernating frogs:mitochondrial proton leak during metabolic depression
Authors: Boutilier Robert G. , St-Pierre Julie 
Published: April 2021

SUMMARYThe common frog (Rana temporaria) spends the coldest months of each year overwintering in ice-covered ponds where temperatures can vary from 0.5 to 4.0°C. Over the course of a winter season, the animals enter progressively into a state of metabolic depression that relies almost exclusively on aerobic production of ATP. However, if aerobic metabolism is threatened, for example by increasingly hypoxic conditions, decreases in the animal's metabolic rate can reach upwards of 75% compared with the 50%decrease seen during normoxia. Under these conditions, the major proportion of the overall reduction in whole-animal metabolic rate can be accounted for by metabolic suppression of the skeletal muscle (which makes up approximately 40%of body mass). Little is known about the properties of mitochondria during prolonged periods of metabolic depression, so we have examined several aspects of mitochondrial metabolism in the skeletal muscle of frogs over periods of hibernation of up to 4 months. Mitochondria isolated from the skeletal muscle of frogs hibernating in hypoxic water show a considerable reorganisation of function compared with those isolated from normoxic submerged animals at the same temperature (3°C). Both the active (state 3) and resting (state 4)respiration rates of mitochondria decrease during hypoxic, but not normoxic,hibernation. In addition, the affinity of mitochondria for oxygen increases during periods of acute hypoxic stress during normoxic hibernation as well as during long-term hibernation in hypoxic water. The decrease in mitochondrial state 4 respiration rates during hypoxic hibernation evidently occurs through a reduction in electron-transport chain activity, not through a lowered proton conductance of the mitochondrial inner membrane. The reduced aerobic capacity of frog skeletal muscle during hypoxic hibernation is accompanied by lowered activities of key enzymes of mitochondrial metabolism caused by changes in the intrinsic properties of the mitochondria. In the absence of oxygen, the mitochondrial F1Fo-ATPase (the ATP synthase) begins to run backwards as it actively pumps protons from the matrix in an attempt to maintain the mitochondrial membrane potential. At this time, the ATP synthase functions as an ATPase to preserve a certain proton-motive force. Frogs limit ATP wastage during anoxia by a profound inhibition of the ATP synthase. Taken together, our studies show that protonmotive force is lowered aerobically by restricting electron supply and during anoxia by restricting mitochondrial ATPase activity.
Maintaining muscle mass during extended disuse: aestivating frogs as a model species
Authors: Hudson Nicholas J. , Franklin Craig E. 
Published: April 2021

SUMMARYProlonged muscle disuse in vertebrates can lead to a pathological change resulting in muscle wasting and a loss of muscle strength. In this paper, we review muscle disuse atrophy in the vertebrates and examine the factors that influence the magnitude of the atrophic response during extended periods of inactivity, both artificially imposed (e.g. limb immobilisation) and naturally occurring, such as the quiescence associated with dormancy (e.g. hibernation and aestivation). The severity of muscle atrophy is positively correlated with mass-specific metabolic rate, and the metabolic depression that occurs during dormancy would appear to have a protective role, reducing or preventing muscle atrophy despite periods of inactivity lasting 6-9 months. In the light of these findings, the role of reactive oxygen species and antioxidants during muscle disuse is emphasised.
The ecological and evolutionary interface of hummingbird flight physiology
Authors: Altshuler Douglas L. , Dudley Robert 
Published: April 2021

SUMMARYThe hovering ability, rapidity of maneuvers and upregulated aerobic capacity of hummingbirds have long attracted the interest of flight biologists. The range of intra- and interspecific variation in flight performance among hummingbirds, however, is equally impressive. A dominant theme in hummingbird evolution is progressive invasion of higher-elevation habitats. Hypobaric challenge is met behaviorally through compensatory changes in wingbeat kinematics, particularly in stroke amplitude. Over evolutionary time scales, montane colonization is associated with increases in body mass and relative wing area. Hovering ability has been well-studied in several North American hummingbird taxa, yet the broad range of interspecific variation in hummingbird axial and appendicular anatomy remains to be assessed mechanistically. Such varied features as tail length, molt condition and substantial weight change due to lipid-loading can dramatically alter various features of the flight envelope. Compared with our present knowledge of hovering performance in hummingbirds, the mechanics of forward flight and maneuvers is not well understood.Relationships among flight-related morphology, competitive ability and foraging behavior have been the focus of numerous studies on tropical and temperate hummingbirds. Ecologists have hypothesized that the primary selective agents on hummingbird flight-related morphology are the behaviors involved in floral nectar consumption. However, flight behaviors involved in foraging for insects may also influence the evolution of wing size and shape. Several comparisons of hummingbird communities across elevational gradients suggest that foraging strategies and competitive interactions within and among species vary systematically across elevations as the costs of flight change with body size and wing shape.
Roughness-dependent friction force of the tarsal claw system in the beetlePachnoda marginata(Coleoptera, Scarabaeidae)
Authors: Dai Zhendong , Gorb Stanislav N. , Schwarz Uli 
Published: April 2021

SUMMARYThis paper studies slide-resisting forces generated by claws in the free-walking beetle Pachnoda marginata (Coleoptera, Scarabaeoidea)with emphasis on the relationship between the dimension of the claw tip and the substrate texture. To evaluate the force range by which the claw can interact with a substrate, forces generated by the freely moving legs were measured using a load cell force transducer. To obtain information about material properties of the claw, its mechanical strength was tested in a fracture experiment, and the internal structure of the fractured claw material was studied by scanning electron microscopy. The bending stress of the claw was evaluated as 143.4-684.2 MPa, depending on the cross-section model selected. Data from these different approaches led us to propose a model explaining the saturation of friction force with increased texture roughness. The forces are determined by the relative size of the surface roughness Ra (or an average particle diameter) and the diameter of the claw tip. When surface roughness is much bigger than the claw tip diameter, the beetle can grasp surface irregularities and generate a high degree of attachment due to mechanical interlocking with substrate texture. When Ra is lower than or comparable to the claw tip diameter, the frictional properties of the contact between claw and substrate particles play a key role in the generation of the friction force.
Behavioural and neuroendocrine effects of environmental background colour and social interaction in Arctic charr (Salvelinus alpinus)
Authors: Höglund Erik , Balm Paul H. M. , Winberg Svante 
Published: April 2021

SUMMARYIn salmonid fish, a darker skin colour has been suggested to signal social subordination. Substratum colour is another factor affecting skin pigmentation in fish; in the present experiment, juvenile Arctic charr (Salvelinus alpinus) were acclimated and allowed to interact in pairs for 5 days on a pale or dark background colour. Skin darkness was quantified prior to and following social interaction. Furthermore, agonistic behaviour and skin darkness were quantified, together with plasma levels of cortisol,adrenocorticotropin (ACTH) and α-melanocyte-stimulating hormone(α-MSH), and brain levels of monoamines and monoamine metabolites. The results show that fish interacting on a white background were more aggressive than those interacting on a black background. Social subordination resulted in skin darkening in fish kept on a white background, but not in fish kept on a black background. Furthermore, subordinate fish on a white background showed an elevation of brain norepinephric activity, an effect not seen in subordinate fish on a black background. Subordinate fish on both white and black backgrounds showed a similar activation of the brain serotonergic system and the hypothalamic—pituitary—interrenal axis. These results support the suggestion that skin darkening in subordinates acts as a social signal announcing social submission.
The C-start escape response ofPolypterus senegalus: bilateral muscle activity and variation during stage 1 and 2
Authors: Tytell Eric D. , Lauder George V. 
Published: April 2021

SUMMARYThe fast-start escape response is the primary reflexive escape mechanism in a wide phylogenetic range of fishes. To add detail to previously reported novel muscle activity patterns during the escape response of the bichir, Polypterus, we analyzed escape kinematics and muscle activity patterns in Polypterus senegalus using high-speed video and electromyography (EMG). Five fish were filmed at 250 Hz while synchronously recording white muscle activity at five sites on both sides of the body simultaneously (10 sites in total). Body wave speed and center of mass velocity, acceleration and curvature were calculated from digitized outlines. Six EMG variables per channel were also measured to characterize the motor pattern. P. senegalus shows a wide range of activity patterns, from very strong responses, in which the head often touched the tail, to very weak responses. This variation in strength is significantly correlated with the stimulus and is mechanically driven by changes in stage 1 muscle activity duration. Besides these changes in duration, the stage 1 muscle activity is unusual because it has strong bilateral activity, although the observed contralateral activity is significantly weaker and shorter in duration than ipsilateral activity. Bilateral activity may stiffen the body, but it does so by a constant amount over the variation we observed; therefore, P. senegalus does not modulate fast-start wave speed by changing body stiffness. Escape responses almost always have stage 2 contralateral muscle activity, often only in the anterior third of the body. The magnitude of the stage 2 activity is the primary predictor of final escape velocity.
Honeybee nestmate recognition: the thermal behaviour of guards and their examinees
Authors: Stabentheiner Anton , Kovac Helmut , Schmaranzer Sigurd 
Published: April 2021

SUMMARYIn honeybee colonies, guards protect their nest from various robbers including bees from other colonies. Infrared thermography showed that the guards and the bees examined by them (examinees) differ considerably in their thermal behaviour according to their particular role in the nestmate recognition process. The thorax surface temperature was on average higher and more variable in the examinees (36.1 °C, S.D.=4.14, N=1545, 303 bees) than in the guards (34.0 °C, S.D.=2.00, N=1681, 772 bees). During thorough examinations lasting longer than 30 s, more than 60 % of the examinees showed phases of intense thoracic heating of more than 2 °C(maximum temperature 48.5 °C), whereas most guards cooled down. Our data suggest that these examinees heat up their surface to enhance chemical signalling during examinations.
Cloning and characterization of the heart muscle isoform of sarco/endoplasmic reticulum Ca2+ ATPase (SERCA) from crayfish
Authors: Chen Dongdong , Zhang Zhiping , Wheatly Michele G. , Gao Yongping 
Published: April 2021

SUMMARY
This paper describes the cloning and functional characterization of the heart muscle isoform of Sarco/endoplasmic reticulum Ca2+-ATPase(SERCA) from crayfish Procambarus clarkii. The complete crayfish heart SERCA, identified by reverse transcription-polymerase chain reaction(RT-PCR) and rapid amplification of cDNA ends (RACE), consists of 4495 bp with a 3060 bp open reading frame, coding for 1020 amino acids. This isoform differs from the previously identified axial abdominal (tail) muscle SERCA solely in its C-terminal amino acids. The last nine amino acids of the tail muscle isoform are replaced by 27 hydrophobic amino acids in the heart isoform that have the potential to form an additional transmembrane domain. Consistent with other invertebrate studies, Southern blot analysis suggested that the heart and tail muscle isoforms are encoded from the same gene that is equally related to SERCA-1, -2 and -3 of vertebrates. The tissue distributions of these two isoforms have been assessed using isoform-specific probes and northern analysis. A cardiac-specific probe bound only to a 5.8 kb species in heart and had minimal cross-hybridization with 7.6 and 5.8 kb species in eggs and no hybridization with tail muscle. A tail-isoform-specific probe hybridized with a 4.5 kb species in tail muscle and cross-hybridized with a 4.5 kb species in eggs and 8.8 kb in heart muscle. Both isoforms are expressed in eggs suggesting that transcripts are formed early in development and are subsequently broadly expressed in all tissue types. Expression of the cardiac muscle SERCA isoform varied with the stage of moulting. Expression was high in intermoult and decreased in premoult. However, expression was restored rapidly in postmoult (within 2 days) unlike expression of tail muscle SERCA,which remained downregulated for weeks. Differences in contractility between the two muscle types in the postmoult period may explain these expression patterns.
Intracardiac flow separation in anin situperfused heart from Burmese pythonPython molurus
Authors: Wang Tobias , Altimiras Jordi , Axelsson Michael 
Published: April 2021

SUMMARYThe heart of non-crocodilian reptiles has two separate atria that receive blood from the systemic and pulmonary circulations. The ventricle is not fully divided, but is compartmentalised into two chambers (cavum dorsale and cavum pulmonale) by a muscular ridge that runs from the apex to the base of the ventricle. The muscular ridge is small in turtles, but is well developed in varanid lizards and many species of snakes. These anatomical differences correlate with an effective blood flow separation in varanid lizards, whereas turtles can exhibit very large cardiac shunts. Very little is known about the cardiac shunt patterns in other groups of reptiles.Here we characterise cardiac performance and flow dynamics in the Burmese python (Python molurus) using an in situ perfused heart preparation. The pericardium remained intact and the two atria were perfused separately (Ringer solution), and the two systemic and the pulmonary outflows were independently cannulated. Right and left atrial filling pressures and ventricular outflow pressures of the pulmonary and systemic vessels could be manipulated independently, permitting the establishment of large experimental intraventricular pressure gradients across the muscular ridge. The maximal power output generated by the systemic side of the ventricle exceeded the maximal power output that was generated by the cavum pulmonale that perfuse the pulmonary circulation. Furthermore, systemic flow could be generated against a higher outflow pressure than pulmonary flow. Perfusate entering the right atrium was preferentially distributed into the pulmonary circulation,whereas perfusate into the left atrium was distributed to the systemic circulation.Our study indicates that the well-developed muscular ridge can separate the cavum systemic and pulmonary sides of the heart to prevent mixing of systemic and pulmonary flows. Therefore, the heart of Python appears to exhibit a large degree of ventricular flow separation as previously described for varanid lizards. We speculate that the ventricular separation has evolved in response to the need of maintaining high oxygen delivery while protecting the pulmonary circulation from oedema as result of high vascular pressures.
Glycerol and NEFA kinetics in long-term fasting king penguins: phase IIversusphase III
Authors: Bernard S. F. , Fayolle C. , Robin J.-P. , Groscolas R. 
Published: April 2021

SUMMARYIn spontaneously fasting birds such as penguins, below a body mass threshold corresponding to the phase II—phase III transition, a metabolic and hormonal shift occurs and feeding behaviour is stimulated(`refeeding signal'). The major aim of this study was to determine whether a decrease in non-esterified fatty acid (NEFA) release from adipose tissue could be a component of this signal. Lipolytic fluxes and primary triacylglycerol:fatty acid (TAG:FA) cycling were determined in vivoin breeding, fasting king penguins (Aptenodytes patagonicus) using continuous infusions of 2-[3H]glycerol and 1-[14C]palmitate under field conditions. In phase II (after approximately 8 days of fasting, large fat stores, body protein spared, N=8), the rate of appearance (Ra) of glycerol and of NEFA were 5.7±0.8 and 10.5±0.4 μmol kg-1min-1, respectively, and the percentage of primary TAG:FA cycling was 41±7%. In phase III (after approximately 25 days of fasting, fat stores reduced by fourfold, increased body protein catabolism, N=9), Ra glycerol kg-1 body mass remained unchanged,whereas Ra glycerol kg-1 fat mass and Ra NEFA kg-1 body mass were increased by 2.8-fold and 1.5-fold, respectively. Increased Ra glycerol kg-1 fat mass was possibly the result of a 3.5-fold increase in circulating glucagon, the increased Ra NEFA kg-1 body mass being attributable to decreased primary TAG:FA cycling. Thus, triggering of the refeeding signal that redirects the behavior of fasting, incubating penguins from incubation towards the search for food after entrance into phase III cannot be ascribed to a reduction in lipolytic fluxes and NEFA availability.
Active ammonia excretion across the gills of the green shore crabCarcinus maenas: participation of Na+/K+-ATPase, V-type H+-ATPase and functional microtubules
Authors: Weihrauch Dirk , Ziegler Andreas , Siebers Dietrich , Towle David W. 
Published: April 2021

SUMMARYAlthough aquatic animals are generally believed to export nitrogenous waste by diffusion of NH3 or NH4+ across external epithelia, evidence for active ammonia excretion has been found in a number of species. In the euryhaline green shore crab Carcinus maenas, active excretion of ammonia across isolated gills is reduced by inhibitors of the Na+/K+-ATPase and vacuolar-type H+-ATPase. In addition, a functional dynamic microtubule network is necessary, since application of colchicine, taxol or thiabendazole leads to almost complete blockage of active and gradient-driven ammonia excretion. Actin filaments seem not to play a role in the excretory process. The NH4+-dependent short-circuit current and the conductance of the isolated cuticle were reduced in a dose-dependent manner by amiloride,a non-specific inhibitor of the Na+/H+ exchanger and Na+ channels. Combined with an analysis of gill morphology, the strong intracellular but weak apical abundance of V-type H+-ATPase and the fact that ammonia flux rates are equal under buffered and unbuffered experimental conditions, our observations suggest a hypothetical model of transepithelial ammonia movement that features active uptake across the basolateral membrane, sequestration in acidified vesicles, vesicle transport via microtubules and exocytosis at the apical membrane.
Collision-avoidance and landing responses are mediated by separate pathways in the fruit fly,Drosophila melanogaster
Authors: Tammero Lance F. , Dickinson Michael H. 
Published: April 2021

SUMMARYFlies rely heavily on visual feedback for several aspects of flight control. As a fly approaches an object, the image projected across its retina expands, providing the fly with visual feedback that can be used either to trigger a collision-avoidance maneuver or a landing response. To determine how a fly makes the decision to land on or avoid a looming object, we measured the behaviors generated in response to an expanding image during tethered flight in a visual closed-loop flight arena. During these experiments, each fly varied its wing-stroke kinematics to actively control the azimuth position of a 15°×15° square within its visual field. Periodically, the square symmetrically expanded in both the horizontal and vertical directions. We measured changes in the fly's wing-stroke amplitude and frequency in response to the expanding square while optically tracking the position of its legs to monitor stereotyped landing responses. Although this stimulus could elicit both the landing responses and collision-avoidance reactions, separate pathways appear to mediate the two behaviors. For example, if the square is in the lateral portion of the fly's field of view at the onset of expansion, the fly increases stroke amplitude in one wing while decreasing amplitude in the other, indicative of a collision-avoidance maneuver. In contrast, frontal expansion elicits an increase in wing-beat frequency and leg extension,indicative of a landing response. To further characterize the sensitivity of these responses to expansion rate, we tested a range of expansion velocities from 100 to 10 000° s-1. Differences in the latency of both the collision-avoidance reactions and the landing responses with expansion rate supported the hypothesis that the two behaviors are mediated by separate pathways. To examine the effects of visual feedback on the magnitude and time course of the two behaviors, we presented the stimulus under open-loop conditions, such that the fly's response did not alter the position of the expanding square. From our results we suggest a model that takes into account the spatial sensitivities and temporal latencies of the collision-avoidance and landing responses, and is sufficient to schematically represent how the fly uses integration of motion information in deciding whether to turn or land when confronted with an expanding object.
Exploring the sialome of the tick Ixodes scapularis
Authors: Valenzuela Jesus G. , Francischetti Ivo M. B. , Pham Van My , Garfield Mark K. , Mather Thomas N. , Ribeiro José M. C. 
Published: April 2021

SUMMARY
To attempt description of the set of mRNA and protein (sialome) expressed in the salivary glands of the tick Ixodes scapularis, we randomly sequenced 735 clones of a full-length salivary gland cDNA library of this arthropod and performed Edman degradation of protein bands from salivary gland homogenates (SGH) and saliva separated by SDS-PAGE. The sequences were grouped into 410 clusters, of which 383 are not associated with known I. scapularis sequences. 15- and 17-protein bands from PAGE yielded amino-terminal information on the saliva and salivary gland gels,respectively. We attributed 19 of these sequences to translation products of the cDNA library. Full-length sequences were obtained for 87 clones. Among these protein sequences are several protease inhibitors of distinct classes,metalloproteases, novel proteins with histamine-binding domains, and several peptide families of unknown function displaying different conserved cysteine residues, many of which contain single Kunitz domains. This work provides information into the diversity of messages expressed in the salivary glands of I. scapularis, describes novel sequences that may be responsible for known biological activites, indicates further biological activities that may be present in I. scapularis saliva and identifies novel vaccine targets that may be used in Lyme disease prevention.