bluuebunny/crossref_metadata_2025_split huggingface dataset:
Improved Fertiliser Management To Reduce The Greenhouse-Gas Emissions And Ensure Yields In A Wheat–Peanut Rotation System In China
Authors: Liu Zhaoxin , Zhao Chen , Zhao Jihao , Lai Huajiang , Li Xiangdong 
Published: September 2021

Abstract
Over the last century, anthropogenic greenhouse-gas (GHG) emissions have changed the global climate, and agriculture plays an important role in the global flux of GHG. Agricultural management practices, such as split N applications and the use of controlled-release fertilisers have significantly increased the crop yield and N-use efficiency by balancing the N demand of crops and the N availability of soils. However, the impacts of these practices on GHG emissions (in particular in wheat–peanut relay intercropping systems) have not been evaluated in detail. In this study, we used a common compound fertiliser and a controlled-release compound fertiliser (CRF) applied the day prior to sowing, at the jointing stage of wheat, and at the anthesis stage of peanut in various proportions, with a control treatment of 0 kg ha-1. The findings demonstrated that treatment JCF70 achieved increases in yields of 9.7% and 14.6% for wheat grain and peanut pod, respectively, compared to treatment JCF100; however, this treatment also significantly increased soil emissions of CO2 and N2O. In addition, cumulative emissions of CO2 and N2O were higher in the peanut growing season by 74.4 and 31.7%, respectively, than in the wheat growing season owing to the relatively higher soil temperature during the former season. Fertilisation combined with irrigation, was found to be the main cause of GHG emissions. Under the same fertiliser rate and N-management style, JCRF70 further increased the yield of peanut pods and the total combined yield of peanut and wheat by 10.3% and 8.9%, respectively, compared to treatment JCF70. The cumulative CO2 and N2O emissions in treatment JCRF70 were 20.4–45.4% less than those in treatment JCF70. The total global warming potentials of CO2 and N2O were lowest in treatment JCRF70 owing to it providing the highest grain yield. Therefore, N application with three splits, together with the use of a slow-release fertiliser, may be a simple and effective approach to enhance the grain yield while reducing the GHG emissions.
Tissue fluidification promotes a cGAS/STING-mediated cytosolic DNA response in invasive breast cancer
Authors: Frittoli Emanuela , Palamidessi Andrea , Iannelli Fabio , Zanardi Federica , Villa Stefano , Barzaghi Leonardo , Ando Hind , Cancila Valeria , Beznuskenko Galiba , Chiara Giulia Della , Pagani Massimiliano , Malinverno Chiara , Bhattacharya Dipanjan , Pisati Federica , Yu Weimiao , Galimberti Viviana , Bonizzi Giuseppina , Martini Emanuele , Mironov Alexander , gioia ubaldo , fagagna Fabrizio D'adda di , Rossi Chiara , Bertalot Giovanni , Lucioni Marco , tancredi richard , Pedrazzoli paolo , Vecchione andrea , Perini Cristiuano , Ferrari FRancesco , Lanzuolo Chiara , Nader Guilherme , Foiani Marco , Piel Matthieu , Cerbino Roberto , Giavazzi Fabio , Tripodo Claudio , Scita Giorgio 
Published: September 2021

Abstract
The process in which locally confined epithelial malignancies progressively evolve into invasive cancers is often promoted by unjamming, a phase transition from a solid-like to a liquid-like state that occurs in various tissues. Whether this tissue-level mechanical transition impact phenotypes during carcinoma progression remains unclear. We show, here that the large fluctuations in cell density that accompany unjamming result in repeated mechanical deformations of cells and nuclei. Cells react to these protracted mechanical stresses by mounting a mechano-protective response that includes enlarged nuclear size and rigidity, altered heterochromatin distribution, and the remodeling of the perinuclear actin architecture into actin rings. The chronic strains and stresses associated with unjamming together with reduction of Lamin B1 levels eventually result in DNA damage and nuclear envelope ruptures, with the release of cytosolic DNA that activates a cGAS/STING-dependent cytosolic DNA response gene program. This mechanically-driven transcriptional rewiring ultimately results in a change in cell state, with the emergence of malignant traits, including epithelial-to-mesenchymal plasticity phenotypes and chemo-resistance in invasive breast carcinoma.

One-Sentence Summary: A solid-to-fluid phase transition promotes a pro-inflammatory transcriptional response in invasive breast carcinoma
Range Extension and Species Confirmation of Rhyneptesicus Nasutus (Sind Serotine Bat) (Mammalia: Chiroptera) From Bajaur Agency, FATA, Pakistan
Authors: Idnan Muhammad , Mansoor Sajid , Khawar Muhammad Babar , Javid Arshad , Hussain Ali , Imran Muhammad , Ullah Arif 
Published: September 2021

Abstract
The lack of morphological differentiation among chiropteran species and cryptic speciation impedes species identification. So, DNA-based approaches boost species identification and are increasing biological diversity. Rhyneptesicus nasutus (Sind Serotine Bat) is a rarely distributed and poorly studied species in Pakistan. This study explores the range extension of Sind bat within the territorial limits of Pakistan from Sind and Baluchistan to Federally Administered Areas (FATA) of Pakistan. No molecular record exists for the species in Pakistan. In the present study, we for the first time present the data to confirm the species identification by genetic marker (cytochrome b) along with morphometric analysis of Rhyneptesicus nasutus from Pakistan. The neighbor-joining tree based on Kimura-2 parameters was created to infer the phylogenetic analyses. We sequenced the cytochrome b gene segment and conducted a phylogenetic analysis with previously published data from other countries. Sequences from Pakistan made a clade with Iranian Rhyneptesicus nasutus species suggesting a common ancestry. Various morphometric parameters (mean values) were measured like Head and Body length (44.3mm), Tail length (43.4mm), Hindfoot length (8.3mm), Forearm length (35.7mm), and Ear length 36mm while 5th Metacarpal Length, 4th Metacarpal Length, and 3rd Metacarpal Lengths were 33.2mm, 34.7mm, 35.3mm. Approaches based on DNA barcoding reveals high diversity of bat species in the study area. The data will enable researchers to build an improved evolutionary landscape of the Serotine bats from this region and subsequently reconstruct a detailed evolutionary history of the genus. Further research is required to test other molecular markers to support the findings of the current study in Pakistan.
Effect of Physical Properties of Synthesized Protic Ionic Liquid On Carbon Dioxide Absorption Rate
Authors: Chaudhary Amita , Bhaskarwar Ashok N 
Published: September 2021

Abstract
Concentration of carbon dioxide gas has accelerated from the last two decades which cause drastic changes in the climatic conditions. In industries, carbon capture plants use volatile organic solvent which causes many environmental threats. So, a low-cost green absorbent has been formulated with nontoxicity and high selectivity properties for absorbing carbon dioxide gas. This paper contains the synthesis process along with the structure confirmation using 1H NMR, 13C NMR, FT-IR, and mass spectroscopy. Density, viscosity, and diffusivity are measured at different ranges with standard instruments. The kinetic studies were also conducted in a standard predefined-interface stirred-cell reactor. The kinetic parameters were calculated at different parameters like agitation speeds, absorption temperature, initial concentrations of ionic liquid, and partial pressure of carbon dioxide. The reaction regime of carbon dioxide absorption is found to be in fast reaction kinetics with pseudo first order. The reaction rate and the activation energy of CO2 absorption are experimentally determined in the range of 299 K to 333K with different initial concentrations of ionic liquid (0.1-1.1 kmol/m3). The second order rate constant and activation energy of carbon dioxide absorption in the synthesized ionic liquid is found to be (6385.93 to 12632.01 m3 mol-1 s-1) and 16.61 kJ mol−1 respectively. This solvent has shown great potential to absorb CO2 at large scale.
SETD2 Deficiency and miR-21: Potent Therapeutic Targets in NUT Midline Carcinoma
Authors: YOSHIDA NANA , OKUMURA SHUNSUKE , SASAKI TAKAAKI , CHIBA SHIN-ICHI , SADO MASATOSHI , OYAMA KYOHEI , YOSHIDA RYOHEI , HIRAI NORIKO , MINAMI YOSHINORI , KITADA MASAHIRO , OHSAKI YOSHINOBU 
Published: September 2021

Abstract
BackgroundNuclear protein in testis (NUT) midline carcinoma (NMC) is a rare and highly aggressive tumor with the bromodomain containing 4 protein (BRD4)-NUT (NUTM1) gene fusion. Few targeted therapies are available for NMC, and novel therapeutic targets are required. The objectives of our study was to determine gene mutations and microRNA (miRNA) expression levels in NMC and to identify novel therapeutic targets for NMC. Methods Next-generation sequencing (NGS) was used to identify mutations in the NMC cell lines and the tumor sample from a NMC patient; we determined the BRD4-NUT fusion gene in the patient using a digital PCR assay. Trimethylation of lysine 36 on histone H3 expression (H3K36me3) was studied using western blotting. The efficacy of the WEE1 G2 checkpoint kinase (WEE1) inhibitor, AZD1775, was evaluated using the MTS assay. RNA sequencing was performed to determine miRNA expression levels in the NMC cell lines. TaqMan miRNA assays were used to analyze miR-21 expression. The efficacy of miR-21 inhibitor was evaluated using the MTS assay. Results A novel SETD2 mutation (p.Ser2382fs) was identified in the NMC cell lines and the patient tumor. H3K36me3 was depleted in the NMC cells, which was indicative of functional SETD2 loss. The NMC cells were sensitive to AZD1775, an inhibitor for SETD2-deficient cancer. miRNA analysis revealed that miR-21 expression was increased in the NMC cells resistant to the traditional BET-targeted therapy. The miR-21 inhibitor suppressed the proliferation of the NMC cells. ConclusionsSETD2 deficiency and miR-21 may serve as novel therapeutic targets for the treatment of NMC.Trial registrationIn this study, we analyzed the gene alterations in human tumor samples. This study was registered in the UMIN clinical trial registered system (UMIN000043147, January 27, 2021).
Prevalence of Hypothyroidism Among Patients With COVID-19, Tehran, Iran
Authors: Esfahanian Fatemeh , SeyedAlinaghi SeyedAhmad , Janfaza Nazanin , Tantuoyir Marcarious M. 
Published: September 2021

Abstract
Objective: Numerous comorbidities are involved in the severity of Corona Virus Disease 2019 (COVID-19). Hypothyroidism's impact on COVID-19 is yet to be understood properly. Cellular immunity and different cytokines, on the other hand, are thought to play a role in the development and progress of COVID-19 and thyroid disease, according to earlier research. The goal of this study was to find out how common (prevalent) hypothyroidism was among COVID-19 patients and its possible influence on the disease prognosis on hospitalized patients at a Tehran University Hospital.Methods: Demographic information and other data related to our study, mainly comorbidities, were collected from 493 COVID-19 patients’ medical records and analyzed.Results: We identified that hypothyroidism was significantly prevalent in hospitalized COVID-19 patients than in the general population. Overall, in patients with hypothyroidism (n=65), n=21(32.3%) patients expired of whom 14 (66.7%) patients were females and 7 (33.3%) were males. In these patients, no statistically significant difference was observed between the expired and discharged groups (Adjusted Odds Ratio (ORadj): 1.04 (95% Confidence Interval (CI): 0.59-1.83 p-value=0.87). As a result, hypothyroidism was not linked to an increased risk of death.Conclusion: In this current study, we demonstrated that the prevalence of hypothyroidism was higher in hospitalized COVID-19 patients than in the normal population especially in females but without significant adverse effects on the risk of mortality from this disease.
Long Noncoding RNA KCNQ1OT1 Induces Resistance of HCC Cells To Cisplatin Through Regulating The miR-26a/CCND2 Molecular Axis
Authors: LI Cai , YE Qi-Fa 
Published: September 2021

Abstract
Objective: To explore the molecular mechanism by which LncRNA KCNQ1OT1 regulated the miR-26a/CCND2 molecular axis to participate in the resistance of Hepatocellular carcinoma(HCC) cells to cisplatin.Methods: Cancer tissue and corresponding para-carcinoma tissue specimens were collected from 25 HCC patients with complete data admitted from January 2018 to December 2018 at The Transplantation Center of the Third Xiangya Hospital. Then, the expression levels of KCNQ1OT1, miR-26a and CCND2 in HCCtissues and cell lines were detected through qRT-PCR. Meanwhile, the sensitivity of HCC cells to cisplatin was examined through Transwell and Annexin V-FITC/PI double staining flow cytometry. Further, the targeted relationships among KCNQ1OT1, miR-26a and CCND were verified through dual-luciferase reporter gene assay, and the regulatory relationships were detected through Western blotting and qRT-PCR.Results: KCNQ1OT1 was highly expressed in HCC tissues and cisplatin-resistant cell lines; meanwhile, over-expression of KCNQ1OT1 promoted the resistance of Huh7/CDDP cells to cisplatin. Dual-luciferase reporter gene assay verified that, KCNQ1OT1 targeted miR-26a and down-regulated its expression level. miR-26a suppressed Huh7/CDDP cell proliferation and invasion, while promoting their apoptosis, thus down-regulating the promoting effect of KCNQ1OT1 on the cisplatin resistance of HCC cells. miR-26a negatively regulated CCND2 expression, while KCNQ1OT1 down-regulated the suppression of miR-26a on CCND2 to promote Huh7/CDDP cell proliferation and invasion and to suppress apoptosis, thereby up-regulating the resistance of HCCcells to cisplatin. Conclusions: LncRNA KCNQ1OT1 regulates the miR-26a/CCND2 molecular axis to induce the resistance of HCC cells to cisplatin.
Strengthening the Service Experiences of Women Impacted by Gambling-related Intimate Partner Violence.
Authors: O'Mullan Cathy , Hing Nerilee , Nuske Elaine , Breen Helen , Mainey Lydia 
Published: September 2021

Abstract
BackgroundWhile problem gambling does not directly cause intimate partner violence (IPV), it exacerbates that violence significantly. Women experiencing both gambling harm and IPV often find themselves in challenging situations; furthermore, stigma and shame frequently act as barriers to seeking help from health and social service agencies. Despite the links between problem gambling and IPV, little is known about women’s experiences of using support services for both IPV and gambling related issues. This paper explores positive experiences of help-seeking for gambling-related IPV in Australia by adopting a strengths-based research approach. MethodsQualitative, unstructured interviews were conducted for a larger study exploring the nature of the relationship between problem gambling and IPV. To gain new insights into the service experiences of women impacted by gambling related IPV, interviews with 48 women with lived experience of IPV relating to a male partner's gambling, and 24 women with lived experience of IPV relating to their own gambling were reanalysed using thematic analysis.ResultsThree themes emerged from the data signifying or demonstrating strength-based responses: 'Commitment to Integrated and Collaborative Responses'; 'Therapeutic Support'; and 'Instrumental Support'. The themes highlight the importance of recognising the intersectionality of gambling related IPV and supporting the person 'at the centre of the service'. Tangible and instrumental supports, such as emergency accommodation and financial assistance, were also central to the recovery process. ConclusionEffective service responses are dependent on understanding how problem gambling and IPV intersect. Importantly, service providers must recognise and address the many facets of each woman's situation and the shame associated with resolving interdependent and complex issues. Responding to the needs of women impacted by gambling related IPV requires both individual-level awareness and organisational support; recommendations to strengthen service provision are provided.
Preoperative Venous Thromboembolism in Orthopedic Oncology Patients: Incidence, Risk Factors, and D-Dimer Cutoff Value
Authors: Hayashida Kenta , Kawabata Yusuke , Saito Keiju , Fujita Shintaro , Choe Hyonmin , Takeyama Masanobu , Inaba Yutaka 
Published: September 2021

Abstract
Background: Venous thromboembolism (VTE) is known as one of the major complications in patients with malignant tumors and orthopedic disorders. ﻿Patients undergoing surgery for orthopedic oncology are at an increased risk for thromboembolic events. However, there are limited studies regarding the incidence and risk factors for preoperative VTE in orthopedic oncology patients.Methods: The aim is to determine the incidence and risk factors of preoperative VTE by screening with D-dimer. We retrospectively reviewed the medical records of 270 patients who underwent surgical procedures, including biopsy for orthopedic oncology, had preoperative D-dimer levels measured and were subsequently screened for VTE by lower extremity venous ultrasonography and contrast-enhanced computed tomography (CT) scans if necessary. Statistical analyses were performed to examine the incidence and risk factors for VTE. Receiver operating characteristic (ROC) analysis was performed to verify the cutoff value of D-dimer for the diagnosis of VTE.Results: A total of 199 patients (103 with primary soft tissue sarcomas, 38 with primary bone sarcomas, 46 with metastatic tumors and 12 with hematologic malignancies) were included. The value of D-dimer was high in 79 patients. VTE was detected in 19 patients (9.5%). Multivariate analysis indicated that, age ≥60 years (P = 0.021), and tumor location in the lower limbs (P = 0.048) were independent risk factors for VTE. ROC analysis showed that the cutoff value of D-dimer for the diagnosis of VTE was 1.53 µg/mL (sensitivity of 89.5% and specificity of 79.4%).Conclusions: Our study indicated that age and tumor location in the lower limbs were independent risk factors for preoperative VTE in orthopedic oncology patients. D-dimer was not significantly different in the multivariate analysis because D-dimer is affected by a wide variety of conditions, such as malignancy and aging. Patients with high D-dimer levels and the abovementioned risk factors are at increased risk of preoperative VTE, and additional ultrasonography should be considered.Trial registration: Our study was approved by the institutional review board. The registration number is B200600056. The registration date was July 13, 2020.
The Association of Plasma Levels of miR-146a, miR-27a, miR-34a and miR-149 with Coronary Artery Disease
Authors: Mirzaei Ali , Hosseini Ebrahim syud , Hosseinpour sara , Khalvati Bahman , ainabad Farshad safari 
Published: September 2021

Abstract
Background: Coronary artery disease (CAD) is considered to be one of the most pivotal causes of death in the world. Over the past two decades, significant changes occurred in diagnosis, prognosis, and treatment of CAD, which has helped reduce mortality rates. miRNAs are a class of more than 5000 non-encoding RNA molecules (21 to 25 nucleotides across the length) that regulate the complex biological processes. Today, miRNAs are used to study cardiovascular diseases. In the present study, the expression of miR-146a،miR-27a ،miR-149 and miR-34a in plasma suffering from CAD and control group were investigated. Methods and Results: The present research was performed on 30 men with coronary artery stenosis (CAD) and 30 healthy men as controls. The expression levels of miR-146a, miR-27a, miR-149 and miR-34a in the plasma of patients with CAD and control group were measured using real-time PCR. Also, the correlation between the expression of circulating miRNAs levels and biochemical LDL-C, HDL-C, BMI, and cholesterol were evaluated. The expression of miR-27a in plasma of CAD group was higher than control group (p=0.020). The expression of miR-146a was downregulated in CAD patients compared to of non-CAD group (p=0. 026). However, the expression of miR-34a, miR-149 in the plasma of CAD patients were not significantly difference with the control group. In addition to, a direct correlation was found between the expression of miR-146a and HDL-c, the expression of miR-27a and LDL-C and the expression of miR-34a and total cholesterol. Also, the negative correlation between expressions of miR-149 with BMI was reported. Conclusion. The obtained results demonstrated that miRNAs was closely related to biochemical factors and it points out the fact that miRNAs can be applied as a potential strategy for diagnosis and treatment of CAD.
Short Term Effects of Mild Severity COVID-19 on Left Ventricular Functions
Authors: Bektaş Osman , Karagöz Ahmet , Yenerçağ Feyza Nur Topçu , Dereli Seçkin , Kurt Celali , Kaya Yasemin 
Published: September 2021

Abstract
Purpose: We aimed to evaluate long term effects of COVID-19 disease on left ventricular function using speckle tracking echocardiography. Methods: In our study, 96 non-hospitalized patients who were diagnosed with COVID-19 disease in our center between 15 August 2020 and 01 September 2020 and 96 age and gender matched control subjects were included.Results:The mean follow up duration was 137 ± 7 days. White blood cell, neutrophile and lymphocyte were significantly lower and C-reactive protein (CRP) and neutrophil to lymphocyte ratio (NLR) were significantly higher in the COVID-19 group. (6.6 ± 2.8 vs. 24.4 ± 21.8, p <0.001 and 4.1 ± 2.5 vs. 7.1 ± 4, p <0.001, respectively). LV-GLS and LV-GCS were significantly lower in the COVID-19 group. (-20.1 ± 3.1 vs -15.9 ± 2, p <0.001 and -29.3 ± 2.1 vs -26.3 ± 2.1, p <0.001, respectively). LV-GLS and LV-GCS were found to be negatively correlated with CRP (rs = -0.515, p <0.001 and rs = 0.-466, p <0.001, respectively) and NLR (rs = -0.494, p <0.001 and rs = -434, p <0.001, respectively). Conclusion: Deteriorating effects of COVID-19 disease on both LV-GCS and LV-GLS can be seen even in the short term. These detrimental effects seem to be associated with CRP levels and NLR measured in the course of active infection.
Determination of Prostate-Specific Antigen Via The Assembly of a Two-Dimensional Nanoplatform
Authors: Chen Junjie , Li Xiangqian , Yu Xiaoqi , Wang Qianming 
Published: September 2021

Abstract
Two-dimensional platforms with favorable features are highly expected for diverse application. In this work, we report a highly sensitive and selective “turn-on” fluorescent nanoprobe for prostate-specific antigen (PSA) detection base on cobalt oxyhydroxide nanosheets (CoOOH NSs). CoOOH NSs are employed as the suitable sensing hosts, in which fluorescein amidite-(abbreviated as FAM) labeled aptamer probe (PA) has been adsorbed on nanosheets. Energy transfer between substrate and optical species has switched off the fluorescence of PA. The strong affinity of PA to the target PSA induces the formation of a rigid aptamer structure and the integration with the CoOOH NSs has been drastically affected. The recognition process has been followed by the release of the aptamer probe PA from the nanosheet surface and the green luminescence has been recovered. The dynamic nano-sensor exhibits highly sensitive and accurate analytical performance toward PSA with a linear detection range from 0.1 to 5 nM and a detection limit of 56.1 pM. Therefore, a simple and efficient sensing platform for the detection of prostate cancer can be established.