bluuebunny/crossref_metadata_2025_split huggingface dataset:
Facile Fabrication of Hybrid Carbon Nanotube Sensors by Laser Direct Transfer
Authors: Bonciu Anca , Filipescu Mihaela , Voicu Stefan , Lippert Thomas , Palla-Papavlu Alexandra 
Published: January 2021

Abstract

This paper describes a rapid, solvent-free laser based procedure for the fabrication of reproducible sensitive sensors based on hybrid nanocomposites, i.e. single walled carbon nanotubes (SWCNTs) decorated with tin oxide nanoparticles (SnO2 NP) that overcomes challenges associated with solvent-assisted chemical functionalization and integration of these materials into devices.
The gas response of the LIFT-ed SWCNT: SnO2 sensors has been evaluated at room temperature and an enhanced 2 fold response to ammonia as compared to the SWCNT sensors is demonstrated. Spontaneous and full recovery of the hybrid nanocomposite signal after exposure to NH3 is achieved without any post treatment. The theoretical detection limit of the LIFT-ed SWCNT: SnO2 sensors at room temperature is calculated to be 0.59 ppb. These results indicate that LIFT is an excellent technique which shows great promise towards advancing future developments in the field of chemical sensors.
HLF is&nbsp;a Potential Prognostic Biomarker in Head and Neck Squamous Cell Carcinoma Based on Bioinformatic Analysis and Experimental Validation
Authors: Fang Wei , Wan Di , Chen Jun , Ma Weiqun , Luo Zhen , Yang XiaoQin 
Published: January 2021

Abstract
BackgroundHead and neck squamous cell carcinoma (HNSCC) is one of the most frequent cancers worldwide, with an increasing incidence. However, the underlying molecular mechanisms of HNSCC are poorly understood.MethodIn this work, 5 original datasets (GSE23558, GSE13601, GSE30784, GSE9844, GSE78060) of Head and neck squamous cell carcinoma (HNSCC) were selected from Gene Expression Omnibus (GEO) database. To identify differentially expressed genes (DEGs) in HNSCC and adjacent tissues. The common DEGs were acquired by Venn diagram. The sensitivity and specificity of HLF were determined by Receiver operating characteristic curves (ROC). Then, In order to further confirm the relationship between HLF and HNSCC patient’s prognosis, the expression and survival analysis of HLF was performed by Gene Expression Profiling Interactive Analysis (GEPIA), Cell culture, reverse transcription polymerase chain reaction (RT-PCR), western blotting and immunohistochemical staining.ResultsSeventeen DEGs were screened from five sets of HNSCC functional gene expression series in GEO datasets. The low expression of HLF was indicated might be correlated with poor prognosis of HNSCC patients based on the bioinformatics analysis. According to the results of Cell culture, RT-PCR, western blotting, immunohistochemical staining, it was confirmed that the low level of HLF expression correlated with poor prognosis of HNSCC patients.ConclusionThe study effectively revealed useful information about the relationship of the low level of HLF expression and HNSCC. In summary, we identified HLF as a potential prognostic biomarker and therapeutic target for HNSCC.
Diffuse peritonitis caused by spontaneous perforation of pyometra: A case report and literature review
Authors: Xu Hao , Zhuang Yifan , Wang Caiming , Zhou Yongjie , Chen Haofei , Li Xin , Zhou Wence 
Published: January 2021

Abstract
BackgroundUterine perforation is commonly caused by iatrogenic injuries, tumours, and traumas. Uterine perforation resulting from infection is less common, and such perforation rarely causes abdominal empyema and diffuse peritonitis. Infectious complications such as shock and multiple organ dysfunction are extremely dangerous and even life threatening. Correct early diagnosis and appropriate treatment can reduce the mortality of pyometra.Case presentationA 62-year-old woman presented to our hospital with persistent abdominal pain for 5 hours. The patient had undergone hysteroscopy six months ago, and a cystic space-occupying lesion of the uterus was found one month ago. Perfect the various examinations, consider the patient as acute diffuse peritonitis Perforation of the digestive tract? Uterine cavity infection. In the emergency department, laparoscopic exploration was performed. During the operation, it was diagnosed as pyometra and perforation of the right uterine fundus. Then, combined with a gynecologist, a hysterectomy with double appendages was performed. The operation went smoothly. The patient had paralytic intestinal obstruction after the operation. After active symptomatic treatment The patient recovered well and was discharged. Postoperative pathology results were as follows: 1. Moderately differentiated squamous cell carcinoma of the cervix (non-keratinizing type); 2. Metastatic squamous cell carcinoma of the right ovary; 3. Perforation of the fundus of the uterus, pyometra, and extensive purulent inflammation of the muscle wall .ConclusionsUterine perforation is a rare cause of acute abdomen. Clinicians often don't know enough about this, and they are prone to misdiagnosis and missed diagnosis. For elderly women with abdominal pain and peritonitis, the possibility of empyema perforation should be considered during diagnosis and treatment, and the examination should be improved to avoid misdiagnosis, because correct early diagnosis and appropriate treatment can reduce the mortality of empyema.
The correlation between gross domestic product per capita and the success rate of assisted reproductive technologies worldwide
Authors: Dong Jie , Yan Song , Qian Chenxi , Wang Xiaohong 
Published: January 2021

Abstract
Background
Although there has been increased utilization of assisted reproductive technologies (ART) in the world, there is no conclusive definition of the relationship between the success rate of ART and national wealth.
Methods
In this study, using the data from the International Committee for Monitoring Assisted Reproductive Technologies (ICMART), we sought to determine whether there is a correlation between the success rate of ART (represented by pregnancy/delivery rates) and national wealth (represented by gross domestic product per capita (GDP)). Moreover, to further understand the effect of GDP on ART effectiveness, we analyzed the association between ART success rate and GDP in 50 US states.
Results
Our data showed that the number of ART treatment cycles increased with an increase in GDP. However, we found a negative correlation between ART success rate and GDP in ICMART countries, but no correlation was seen in the US states. Using rough estimation, we derived that the success rate of ART was not related to GDP in the ICMART countries with GDP per capita more than thirteen thousand dollars.
Conclusions
In this study, for the first time, we show that when the GDP of an economic body reaches (or exceeds) thirteen thousand dollars, its ART pregnancy and delivery rates cannot be associated with GDP, and the ART success rate remains stable.
Is Double-Strut Fibula Ankle Arthrodesis a Reliable Reconstruction for Bone Defect after Distal Tibia Tumor Resection? -A Finite Element Study Based on Promising Clinical Outcomes
Authors: Zhao Zhiqing , Yan Taiqiang , Guo Wei , Yang Rongli , Tang Xiaodong 
Published: January 2021

Abstract
Background: There are different surgical methods for primary malignant tumor located at distal tibia. Previous studies have reported that double-strut fibula ankle arthrodesis is an alternative reconstruction. The purpose of this study was to investigate the biomechanical effect of double-strut fibula ankle arthrodesis by finite element analysis (FEA). Methods: Computer aided design software was used to established three-dimension models. Three different models were construct: normal tibia-fibula-talus complex (Model A), double-strut fibula ankle arthrodesis (Model B), and reconstruction by ipsilateral fibula (Model C). We used FEA to evaluate and compared the biomechanical characteristics of these constructs. Simulated loads of 600 N was applied to the tibial plateau to simulate balanced single-foot standing. Output results representing the model von Mises stress, and displacement of the components were analyzed.Results: Construct stiffness was significantly increased when the internal plate fixation was used. For axial loads, Model B (963.79 N/mm) was significantly stiffer than the construct of Model A (430.76 N/mm), and Model C (616.06 N/mm), indication Model B was more stable. Maximum stress on the fibular graft occurred on the proximal end. There were no significant differences in von Mises stress and stress distribution of fibular graft in Model B (22.73 MPa) and Model C (23.69 MPa). In Model B, the ipsilateral fibula in Model B has a higher value of stress (12.07 MPa) than that in Model A (0.67 MPa), indicating the ipsilateral fibula shared load after fusion with talus. For axial load, displacement at the fibular graft in Model B (0.37 mm) was significantly less than that in Model C (0.82 mm).Conclusions: Our computational findings suggest that double-strut fibula ankle arthrodesis is an acceptable construct for distal tibia defect and the ipsilateral fibula shared load after fusion with talus.
Angiotensin-(1-7) Oral Formulation Improves Physical Performance in Mountain Bike Athletes: a Double-blinded Crossover Study
Authors: Moura Samara Silva de , Mendes Adália Táci Pereira , Júnior Francisco de Assis Dias Martins , Totou Nádia Lúcia , Coelho Daniel Barbosa , Oliveira Emerson Cruz , Motta-Santos Daisy , Santos Robson Augusto Souza dos , Becker Lenice Kappes 
Published: January 2021

Abstract
Background: The ECA2/Ang-(1-7)/Mas axis is shown to be involved in effects mediated by physical exercise, as it can induce the release of nitric oxide (ON) and bradykinin (BK), which are potent vasodilators. The vasodilating action the NO/BK can contribute to increased metabolic efficiency in muscle tissue and central nervous system. The formulation HPβ-CD-Ang-(1-7) through its mechanisms of action can be a promising supplement to aid in the maintenance and improvement of performance and may also favor recovery during competitions. The premise of this study was to investigate the effects of acute oral supplementation HPβ-CD-Ang-(1-7) on the performance of mountain bike (MTB) practitioners. Methods: Fourteen recreational athletes, involved in training programs for at least one year, participated in this crossover design study. Subjects underwent two days of testing with a seven-day interval. HPβ-CD-Ang-(1-7) (1.75 mg) and HPβCD-Placebo were provided in capsules three hours prior to tests. To determine the safety of the HPβ-CD-Ang-(1-7) formulation associated with physical effort, cardiovascular parameters heart rate (HR) and blood pressure (BP) were analyzed. Physical performance was measured using maximal oxygen uptake (VO2), total exercise time (TET), mechanical work (MW), mechanical efficiency (ME), and rating of perceived exertion (RPE). Respiratory exchange coefficient (REC), lactate and non-esterified fatty acids (NEFAs) were measured. Maximal incremental tests were performed on a progressively loaded leg cycle ergometer. Results: There were no significant differences in terms of HR or BP at rest and maximum effort between the HPβ-CD-Ang-(1-7) and placebo groups. The VO2max showed significant differences (p = 0.04). It was higher in the Ang-(1-7)condition (66.15 mlO2.kg-1.min-1) compared to the placebo (60.72 mlO2.kg-1.min-1). This was also observed for TET (Ang-(1-7) 39.10 min vs. placebo 38.14 min; p = 0.04), MW (Ang-(1-7) 156.7 vs. placebo 148.2; p = 0.04), and at the lowest RPE (Ang-(1-7) vs. placebo; p = 0.009). No significant differences were observed for REC, NEFAs, or Lactate.Conclusion: These results suggest that HPβ-CD-Ang-(1-7) improves the physical performance of MTB recreational athletes and could be a promising supplement.Trial registration: RBR-2x56pw8, registered January 15th, 2021.
Relationship between Suicidal Ideation and Stigma, Disclosure, and Perceived Social Support in HIV /AIDS Patients
Authors: Yu Yong 
Published: January 2021

Abstract
Objective: To explore the relationship between suicidal ideation and stigma, disclosure and social support in HIV /AIDS patients.Methods: A total of 1146 HIV-infected /AIDS patients were investigated by a general situation questionnaire, Perceived Social Support Scale (PSSS), Beck Suicidal Ideation Scale (Chinese version) (BSI-CV), disclosure questionnaire and Stigma Scale (SSS-S) on a WeChat platform. Results: 54.01% (619/1146) of the sample reported having suicidal ideation in the past week or at the height of depression; The results of multivariate logistic regression analysis showed that In SS-S, the affective dimension [OR value (95%CI) was 1.63(1.17-2.26)], the cognitive dimension [OR value (95%CI) was 2.06 (1.48-2.87)], the behavioral dimension [OR value (95%CI) was 2.05 (1.53-2.76)], and the family support [OR value (95%CI) was 0.64 (0.47 ~ 0.87)], which were associated with suicidal ideation in HIV /AIDS patients; Social support mediated between AIDS-related stigma and suicidal ideation (19.80% of the total effect). Conclusion: Suicidal ideation in HIV /AIDS patients is strongly associated with stigma, awareness, and perceived social support. So, stigma, awareness, and perceived social support should be incorporated into the suicide intervention system for HIV /AIDS patients.
Prevalenceof Intestinal Parasites and Associated Factors Among Food Handlers Working in Food and Drink Establishments at Mizan-aman Town, Southwest Ethiopia, 2018, Community Based Study
Authors: Dachew Aklilu Mamo , Zegene Girma Mamo , Shanab Esrael Derekiab , Endalew Mesenbet Muluken , Kebede Samuel Sahle 
Published: January 2021

Abstract
Background: Food borne diseases has remained a major global public health issue with substantial morbidity and mortality associated with the consumption of contaminated food staffs. Parasitic infection is among the major disease especially in developing countries and sub-Saharan countries.Objective:-This study was to determine the prevalence of intestinal parasites and associated factors among food handlers working in food and drink establishments at Mizan Aman town southwest Ethiopia.Method:- A community based cross sectional study was conducted in Mizan Aman town. A total of 418 food handlers from randomly selected 209 foods and drink establishments were interviewed and finally stool samples were taken. Parasitological assessment was performed by qualified laboratory technologists using the physiological saline for wet mount and zinc sulphate and formol ether concentration techniques were used. Data were analyzed using statistical SPSS version 21. P ≤0.05 was taken as statistically significantResult: The overall prevalence of intestinal parasites in Mizan Aman town was 26.1% .work experience >10 years (AOR=0.52,95% CI ;2.430,5.012),the food handlers who did not trimmed their finger,(AOR =1.89,95% CI :1.01,3.960) and using common knife for cutting raw meat and other foods (AOR=0.57, 95% CI : 3.61,5.54) were factors associated with parasitic infections. Conclusion: The magnitude of intestinal parasite in mizan aman town is high and work experience, finger trimming status, and using common knife for cutting raw meat and other foods were factors associated with the parasitic infections
Free Leptin Index is Elevated in Preeclamptic but not Healthy Women throughout Gestation. A Prospective Cohort Study
Authors: Leal María Carolina Páez - , Garces Maria Fernanda , Bermudez María Alejandra Cano – , Acosta Luis Miguel Maldonado - , Franco Jhon Jairo Peralta - , Cardenas Álvaro Javier Burgos - , Müller Edith Ángel - , Baños Arturo José Parada – , Schmalbach Javier Eslava – , Navarro Haiver Antonio Rodríguez - , Acosta Julieth Daniela Buell - , Castaño Justo , Nogueiras Rubén , Dieguez Carlos , Sanchez Elizabeth , Parra Ariel Iván Ruiz – , Caminos Jorge Eduardo 
Published: January 2021

Abstract
The ratio leptin/soluble leptin receptor (sOB-r), free leptin index (FLI), is used as a marker of leptin sensitivity/resistance in different pathologies. The aim of this study was to evaluate FLI in healthy non-pregnant, healthy pregnant and mild preeclamptic women during pregnancy. We conducted a nested case-control study within a longitudinal observational prospective cohort study. Serum leptin (p=0.0001) and sOB-r (p=0.0000) levels rose significantly throughout pregnancy in healthy pregnant and preeclamptic women [leptin (p=0.0000); sOB-r (p=0.0380)]. Serum leptin levels were significantly higher in preeclamptic compared to healthy pregnant women at 2nd (p=0.0245) and 3rd trimesters of pregnancy (p=0.0016). Additionally, serum sOB-r levels were significantly lower in preeclamptic women during the 2nd (p=0.0236) and 3rd trimester (p=0.0024) of pregnancy compared to healthy pregnant women. Moreover, we found that FLI did not vary significantly during any of the three periods studied in healthy pregnant women (p=0.7640), whereas, increased throughout preeclamptic pregnancy (p=0.0037). Indeed, FLI was significantly higher at 2nd (p=0.0053) and 3rd (p=0.0003) trimesters of pregnancy in preeclamptic compared to healthy pregnant women. Additionally, FLI was significantly higher during luteal phase compared to the follicular phase (p=0.0039). These results demonstrate that FLI increases significantly in preeclamptic pregnant women towards the end of pregnancy.
Reverse glycerol catabolism pathway for dihydroxyacetone and glycerol production by Klebsiella pneumoniae
Authors: Sun Shaoqi , Wang Yike , Shu Lin , Lu Xiyang , Wang Qinghui , Zhu Chengang , Shi Jiping , Lye Gary J. , Baganz Frank , Hao Jian 
Published: January 2021

Abstract
Background: Klebsiella pneumoniae is a bacterium that can be used as producer for numerous chemicals. Glycerol can be catabolised by K. pneumoniae and dihydroxyacetone is an intermediate of this catabolism pathway. Here dihydroxyacetone and glycerol were produced from glucose by this bacterium based on a reverse glycerol catabolism pathway. Results: tpiA, encoding triosephosphate isomerase, was knocked out to block the further catabolism of dihydroxyacetone phosphate in the glycolysis. After overexpression of a Corynebacterium glutamicum dihydroxyacetone phosphate dephosphorylase (hdpA), the engineering strain produced remarkable levels of dihydroxyacetone and glycerol from glucose. Further increase in product formation were obtained by knocking out gapA encoding an iosenzyme of glyceraldehyde 3-phosphate dehydrogenase. There are two dihydroxyacetone kinases in K. pneumoniae. They were both disrupted to prevent an inefficient reaction cycle between dihydroxyacetone phosphate and dihydroxyacetone, and the resulting strains had a distinct improvement in dihydroxyacetone and glycerol production. pH 6.0 and low air supplement were identified as the optimal conditions for dihydroxyacetone and glycerol production by K, pneumoniae ΔtpiA-ΔDHAK-hdpA. In fed batch fermentation 23.9 g/L of dihydroxyacetone and 10.8 g/L of glycerol were produced after 91 hours of cultivation, with the total conversion ratio of 0.97 mol/mol glucose.﻿Conclusions: This study provides a novel and highly efficient way of dihydroxyacetone and glycerol production from glucose.
A Qualitative Study Exploring Barriers, Facilitators and Solutions to Equitable Coverage of Preventive Chemotherapy Towards the Control and Elimination of Neglected Tropical Diseases in South Omo Zone, Nomadic Setting of Southern Ethiopia: Stakeholders’ Perspectives and Experiences
Authors: Asfaw Mekuria Asnakew , Hailu Chernet , Beyene Tariku J. 
Published: January 2021

Abstract
BackgroundDespite substantial progress made towards the control and elimination neglected tropical diseases (NTDs) in Ethiopia using preventive chemotherapy (PC) for lymphatic filariasis, onchocerciasis, schistosomiasis, trachoma, and soil-transmitted helminths, its implementation is facing challenges to achieve equitable coverage especially under mobile populations. This study, therefore, aimed at exploring multiple stakeholders’ perspectives and experiences on barriers, facilitators, and potential solutions to equitable coverage of PC in a mobile nomadic setting of Southern Ethiopia.MethodsThis study was conducted in the South Omo zone in August 2019. Qualitative data collection methods involving key informant interviews (KIIs) and focus group discussions (FGDs) were used to collect data from a total of 27 KIIs and 16 FGDs in a nomadic community of Southern Ethiopia using a semi-structured tool. Data were analyzed using a thematic approach, supported by NVivo 11 software.ResultsThis study revealed multiple barriers affecting equitable access and use of PC, and its facilitators and possible solutions were revealed by taking four dimensions (availability, accessibility, acceptability, and effective coverage) into consideration. The emerged barriers were lack of resource and infrastructure; inadequate information, mobilization and community engagement, lack of awareness, inappropriate scheduling of MDA, distribution of drugs at central point, mobile nature of nomadic communities, security challenges, low school attendance, misconception, misbelief, distrust of government, rumor and drugs’ side-effects, lack of community drug distributors’ (CDDs’) motivation, weak leadership and supportive system. On the other hand, the emerged facilitators were the provision of drugs at no-cost, partners' support, the presence of the religious and cultural organization, and decentralization of services. The possible suggested solutions were increasing partnership, improve supervision, engaging parents in mass drug administration (MDA), arrange a visiting schedule out of working hours, involve the village chief in training, intensify awareness creation, home-to-home visit, and plan mop-up activities.ConclusionsThere is a need of addressing barriers that affect efforts in equitable implementation of MDA to ensure no one is left behind from PC in nomadic settings of Southern Ethiopia. Most importantly, information, mobilization and community engagement, mobile nature of the nomadic community, and high level of low school attendance should take special consideration when MDA is planned in these settings.