bluuebunny/crossref_metadata_2025_split huggingface dataset:
Need for Increased Investment in Human Resource for Health in India: Estimating the Required Investment for Increased Production of Health Professionals for Achieving Universal Health Coverage and Sustainable Development Goals by 2030.
Authors: Karan Anup , Negandhi Himanshu , Kabeer Mehnaz , Zapata Tomas , Mairembam Dilip , De Graeve Hilde , Buchan James , Zodpey Sanjay 
Published: November 2021

Abstract
BACKGROUND: COVID-19 has reinforced the importance of having sufficient, well-distributed and competent health workforce. In addition to improving health outcomes, increased investment has the potential to generate employment, increase labour productivity along with fostering economic growth. With COVID-19 highlighting the gaps in human resources for health in India, there is a need to better and empirically understand the level of required investment for increasing the production of health workforce in India for achieving the UHC/SDGs.METHODS: The study used data from a range of sources including National Health Workforce Account 2018, Periodic Labour Force Survey 2018-19, population projection of Census of India, and review of government documents and reports. The study estimated shortages in the health workforce and required investments to achieve recommended health worker: population ratio thresholds by the terminal year of the SDGs 2030.RESULTS: Our results suggest that to meet the threshold of 34.5 skilled health worker per 10,000 population, there will be a shortfall of 0.16 million doctors and 0.65 nurses/midwives in the total stock of human resources for health by the year 2030. The shortages at the same threshold will be much higher (0.57 million doctors and 1.98 million nurses/midwives) in active health workforce by 2030. The shortages are even higher when compared with a higher threshold of 44.5 health workers per 10,000 population. The estimated investment for the required increase in the production of health workforce ranges from INR 523 billion to 2,580 billion for doctors. For nurses/midwives, the required investment is INR 1,096 billion. Such investment during 2021-25 has the potential of an additional employment generation within the health sector to the tune of 5.4 million and contribute to national income to the extent of INR 3,429 billion annually.Conclusion: India needs to significantly increase the production of doctors and nurses(/midwives) through investing in opening up of new medical colleges. Nursing sector should be prioritized to encourage talents to join nursing profession and provide quality education. India needs to set-up a benchmark of skill-mix ratio and provide attractive employment opportunities in health sector to increase the demand and absorb the new supply of graduates.
The Quality of Palliative Sedation in End-Stage Disease: Audit From a Department of Oncology and Haematology
Authors: Alessia Saviola , Matilde Schipilliti Francesca , Chrystel Isca , Salati Massimiliano , Dini Daniele , Fiorani Claudia , Postiglione Raffaella , Cantile Flavia , Ferrara Leonardo , Carboni Chiara , Potenza Leonardo , Dominici Massimo , Luppi Mario , Longo Giuseppe 
Published: November 2021

Abstract
PurposePalliative sedation (PS) plays a critical role to give suffering relief from refractory symptoms at the end of life. Our audit aimed to assess and improve quality of PS at the Department of Oncology and Hematology of University Hospital of Modena, to verify the adherence to international guidelines, the cooperation among members of care team, focusing with attention on family’s perception of this delicate situation. MethodsFrom December 2016 to June 2019, data of patients undergoing PS in the Department were collected by an electronic folder tool, “Sedation Tool” (ST), that recorded clinical and PS informations, D-PaP, Rudkin Score and family’s perception. Results245 patients were enrolled. 82% had a Karnofsky Performance Status 10-20%. The most common cancer types were lung and gastro-intestinal carcinomas (27% and 21% respectively). Refractory symptoms observed were confusion and agitation (76%), dyspnea (39%), pain (15%), delirium (10%), psychological distress (5%). Midazolam was the drug of choice for PS. Most of patients had Rudkin score 5 after 24 hours and 33% had terminal event within a period of 24 hours from the beginning of PS. During PS most of Patient’s relatives reported peacefulness (65%), agitation/impatience in 6% of cases, concern for suffering (16%). ConclusionPS is used in case of worsening general conditions at the end-stage disease to relieve refractory symptoms with dignity. The ST can become a simple instrument to evaluate and improve PS quality, providing more attention on the impact of PS on relatives to then possibly develop new supportive procedures for patients and their families.
Emergence And Circulation of Dengue Virus Serotype 2 In Guangzhou Over A Period of 20 Years
Authors: Jiang Liyun , Liu Yuan , Su Wenzhe , Cao Yimin , Jing Qinlong , Wu Xinwei , Yang Zhicong 
Published: November 2021

Abstract
Objectives The dengue epidemic in Guangzhou has imposed a rising burden on society and health infrastructure. Here we present the genotype data for dengue virus serotype 2 (DENV-2) to improve the understanding of dengue epidemic. Methods We sequenced the envelope gene of DENV-2 obtained from patient serum sample, and subsequently performed the maximum-likelihood phylogenetic analysis using PhyMLv3.1, the maximum clade credibility analysis using BEAST v.1.10.4 and selection pressure analysis using Datamonkey 2.0 . Results The DENV-2 prevalent in Guangzhou region related to the strains of Southeast Asian countries.Our results suggest that the Malaysia/Indian subcontinent genotype is prevalent in Guangzhou and no genotype shift has occurred during the last 20 years. Episodic positive selection was detected at one site. Conclusions Prevention and monitoring imported cases are important for local control. The shift between the lineages of the Malaysia/Indian subcontinent genotype, which originated at different time points, may be the underlying cause of rising DENV-2 cases in Guangzhou.The low rate of dengue haemorrhagic fever in Guangzhou may be explained by the dominance of the less virulent Malaysia/Indian subcontinent genotype.
A High-Speed Current Source for Magnetorheological Application
Authors: Lei Xie , Wang Yuhao , Lu Chuan , Yang Zhipeng , Liao Changrong 
Published: November 2021

Abstract
Current source is an indispensable component of magnetorheological (MR) systems. Though MR fluid has a phase change as fast as in 1 ms, the response of MR damper (MRD) to generate the damping force may be two orders of magnitude longer. Therefore, the rapid response of current source is a key to realize the real-time semi-active control of MR devices. This study proposes a programmable high-speed, low-cost current source exclusively for MR devices based on the synergy between supercapacitor and Buck converter (i.e., SSBC current source). SSBC current source features a strategy consisting of a lifting phase of supercapacitor and a following maintaining phase of Buck converter. Specifically, the high power density of supercapacitor contributes to rapidly lifting/raising the initial current, and then, like a “relay race”, the expected output is maintained through a Buck converter. Theoretical modeling and experiments are performed systematically. The response times (@ 95% of expected outputs) measured are 0.44, 0.84 and 1.88 ms for the outputs of 3, 6 and 9 A, respectively; these values are highlighted as the fastest level in this field. Besides, the response can be up to 24.6 and 43.7 times faster than the cases using supercapacitor and Buck converter to directly drive the MRD, respectively. SSBC current source is employed to generate a sequence of currents/magnetic inductions, only four variables of which need to be controlled programmatically: the order of lifting and maintaining phases, switching time of lifting phase, PWM duty cycle of Buck converter and duration of maintaining phase. The response time stability is verified by 100 cycles of on/off tests, showing a fluctuation of only 1.1%, which indicates a very reliable high-speed response. This study provides an exclusive power supply with a novel strategy for MR devices, which is believed to be an important promotion for MR technologies.
Cholangiocyte-Derived Exosomal Long Noncoding RNA PICALM-AU1 Promotes Pulmonary Endothelial Cell EndMT in Hepatopulmonary Syndrome
Authors: Yang Congwen , Yang Yihui , Chen Yang , Huang Jian , Li Caiyi , Li Dan , Tang Xi , Ning Jiaoling , Gu Jianteng , Yi Bin , lu kaizhi 
Published: November 2021

Abstract
Background: Hepatopulmonary syndrome (HPS) is an important clinical problem with limited understanding of disease pathologies. Exosome mediated cell-cell communication can modulate various cellular functions by transferring a variety of intracellular components to target cells. A new lncRNA PICALM-AU1 was found and upregulated in the liver of subjects with HPS. However, the expression and biological functions of the lncRNA PICALM-AU1 are still unknown. Methods: HPS rat model was constructed by common bile duct ligation (CBDL). RNA macroarray was used to analyze the expression differential lncRNAs in HPS rat liver. PICALM-AU1 expression in the serum exosome was measured in 56 HPS patients and in 73 patients with liver cirrhosis but not HPS. qPCR, Fluorescence in situ hybridization were used to analyze PICALM-AU1 expression and location. Virus derived PICALM-AU1 upregulation and down regulation were applied in rats and PMVECs cells. The effects of PICALM-AU1 on PMVECs was determined via CCK8 assay and transwell assay. PICALM-AU1 and miR144-3p relationship was analysis by Dual-luciferase reporter assay.Results: In this study, we found lncRNA PICALM-AU1 expressed in the cholangiocyte of liver, secreted as exosome into the serum. PICALM-AU1 carrying serum exosomes induced endothelial-mesenchymal transition (EndMT) of PMVECs and promoted lung injury. Furthermore, overexpression of PICALM-AU1 significantly suppressed miR144-3p and subsequently induced ZEB1 expression.Conclusions: Taken together, our findings present a road map of targeting the newly identified cholangiocyte-derived exosomal lncRNA PICALM-AU1 plays a critical role in the pathologic angiogenesis of HPS by promoting EndMT and represents a potential therapeutic target for HPS.
Mechanism of Peony and Licorice and Aconite Decoction in the Treatment of Osteoarthritis Based on Network Pharmacology and Molecular Docking
Authors: li Zhiqiang , Jun Luo 
Published: November 2021

Abstract
Objective: To predict the key molecular mechanism of Shaoyao Liquorice Aconite Decoction in the treatment of osteoarthritis by using network pharmacology and molecular docking technology, and to provide a new target for the treatment of osteoarthritis. Methods: by means of traditional Chinese medicine database TCMSP screening peony licorice monkshood soup main active component of radix paeoniae alba, radix glycyrrhizae, and the corresponding targets, lateral root of aconite and retrieve OMIM, GeneCards, TDD, PharmGKB and Drugbank database related target for treatment of osteoarthritis, and then forecast drug targets and disease targets for intersection get peony licorice monkshood soup targets for the treatment of osteoarthritis.Then, STRING database and Cytoscape software were used to construct the "drug active component - action target" network and protein interaction network of Shaoyaogaofuzi Decoction in the treatment of osteoarthritis, and David database was used for GO function enrichment analysis and KEGG pathway enrichment analysis of shaoyaogaofuzi Decoction in the treatment of osteoarthritis.Finally, PyMOL, Chem3D, AutoDock, OpenBabel and other software were used to verify the molecular docking of the key active ingredients and key targets of Shaoyao Liquorice Aconite Decoction. Results: 162 active components were screened out.A total of 954 disease targets were collected, and a total of 72 disease targets were obtained after weight removal.Protein interaction analysis suggested that TNF, AKT1, IL6, IL1B and TP53 were the core targets of protein interaction network.Through GO enrichment analysis, 393 biological processes were obtained, and it was found that biological processes were mainly enriched in cell differentiation, migration, apoptosis, and cell stress response to organisms.A total of 116 Pathways were obtained through KEGG pathway enrichment analysis, mainly involving Pathways in cancer, TNF Signaling Pathway, Tuberculosis, Chagas disease, Hepatitis B, etc. Finally, the molecular docking of key active molecules and key targets was realized for verification.Conclusions: this study of compound Chinese medicine pharmacology, through the network of peony licorice monkshood soup ingredients with osteoarthritis, targets, pathway analysis, you can see that drugs in the treatment of osteoarthritis is not a simple single targeted therapy, but by many components, multi-channel, mutual communications between the multiple targets, on the treatment of osteoarthritis in the future to provide more advice.
Impact On Mental Health and Wellbeing in Indigenous Communities Due To Land Loss Resulting From Industrial Resource Development: Protocol for a Systematic Review
Authors: Burns Nicole , Linton Janice , Pollock Nathaniel , Brubacher Laura Jane , Green Nadia , Keeling Arn , Latta Alex , Martin Jessica , Rand Jenny , Ninomiya Melody Morton 
Published: November 2021

Abstract
Background

Indigenous Peoples are impacted by industrial development projects that take place on, or near, their communities. Existing literature on impacts of industrial projects on Indigenous Peoples primarily focus on physical health outcomes and rarely focus on the mental health impacts. To understand the full range of long-term and anticipated health impacts of industrial resource development on Indigenous communities, mental health impacts must be examined. It is well-established that there is a connection between the environment and Indigenous wellbeing, across interrelated dimensions of mental, physical, emotional, and spiritual health. This systematic review will synthesize the evidence on the mental health impacts of land dispossession due to resource extractive projects on Indigenous communities. Looking at the mental health impacts of land dispossession from industrial resource development on Indigenous communities is relevant for a variety of reasons including planning, mitigation strategies, decision making, and negotiations.
Methods

This review includes an Indigenous Advisory Team and a team of Indigenous and settler scholars. The literature search will use the OVID interface to search Medline, Embase, PsycINFO, and Global Health databases. Non-indexed peer reviewed journals related to Indigenous health or research will be scanned. Books and book chapters will be identified in the Scopus and PsycINFO databases. The grey literature search will also include Google and be limited to reports published by government, academic, and non-profit organizations. Reference lists of key publications will be checked for additional relevant publications, including theses, dissertations, reports, and other articles not retrieved in the online searches. Additional sources may be recommended by team members. Included documents will focus on Indigenous Peoples in North America, South America, Australia, Aotearoa New Zealand, and Circumpolar regions, research that reports on mental health, and research that is based on land loss connected to dams, mines, agriculture, oil and gas. Literature that meets the inclusion criteria will be screened at the title/abstract and full text stages by two team members in Covidence. The included literature will be rated with a quality appraisal tool and information will be extracted by two team members; a consensus of information will be reached and be submitted for analysis.
Discussion

The evidence from this review is relevant for land use policy, health impact assessments, economic development, mental health service planning, and communities engaging in development projects.
Systematic review registration:

Registered in the International Prospective Register of Systematic Reviews (PROSPERO; Registration number CRD42021253720)
Lanthanide-Based Metal–Organic Frameworks Solidified by Gelatin-Methacryloyl Hydrogels For Improving the Accuracy of Localization and Excision of Small Pulmonary Nodules
Authors: Ji Haoran , Wang Xiaofeng , Wang Pei , Gong Yan , Wang Yun , Liu Chang , Ji Guangyu , Wang Xiansong , Wang Mingsong 
Published: November 2021

Abstract
The localization of invisible and impalpable small pulmonary nodules has become an important concern during surgery, since current widely used techniques for localization, such as hookwires, microcoils, and indocyanine green (ICG), have a number of limitations. For example, hookwires and microcoils may cause complications because of their invasive features, while ICG undergoes rapid diffusion after injection and has limited application in the localization of deep-seated lesions. In contrast, lanthanide-based metal–organic frameworks (MOFs) have been proven as potential fluorescent agents because of their prominent luminescent characteristics, including large Stokes shifts, high quantum yields, long decay lifetimes, and undisturbed emissive energies. In addition, lanthanides, such as Eu, can efficiently absorb X-rays for CT imaging. In this study, we synthesized Eu-UiO-67-bpy (UiO = University of Oslo, bpy = 2,2'-bipyridyl) as a fluorescent dye with a gelatin-methacryloyl (GelMA) hydrogel as a liquid carrier. The prepared complex exhibits constant fluorescence emission owing to the luminescent characteristics of Eu and the stable structure of UiO-67-bpy with restricted fluorescence diffusion attributed to the photocured GelMA. Furthermore, the hydrogel provides stiffness to make the injection site tactile and improve the accuracy of localization and excision. Finally, our complex enables fluorescence-CT dual-modal imaging of the localization site.
The effect of air bubble position on ART outcome in frozen-thawed blastocyst transfer
Authors: Yang Tingting , Chen Bo , Sun Xiaoyan , Li Qingyang , Li Qiumei , Chen Xuemei , Zhao Li , Pan Ruihua 
Published: November 2021

Abstract
Background

So far, only few literatures have studied the relationship between blastocyst transfer position and ART outcomes, and the conclusions are still controversial. Our study is to evaluate the effect of air bubble position on ART outcome and to find the optimal embryo transfer position in frozen-thawed blastocyst transfer.
Methods

This study included a retrospective cohort analysis of 399 frozen-thawed single blastocyst transfers ultrasound-guided performed between June 1, 2017 and November 30, 2020. All of the women scheduled for frozen-thawed single blastocyst transfers ultrasound-guided. The primary outcome is clinical pregnancy rate and the secondary outcome is live birth rate. Statistical analyses were conducted using One-way Anova, Kruscal Whallis H test, chi-square test and Smooth curve fitting.
Results

When BFD was less than 19 mm, there was no significant change in clinical pregnancy rate as BFD increased (OR = 0.95, 95% CI: 0.89 to 1.02, P = 0.1373); when BFD was more than 19 mm, the clinical pregnancy rate decreased by 16% for every 1 mm increase in BFD (OR = 0.84, 95% CI: 0.72 to 0.98, P = 0.0363). The effect of BFD on live birth rate were similar to that on clinical pregnancy rate, the inflection point was 19mm, when BFD was more than 19 mm, the live birth rate decreases by 58% for every 1 mm increase in BFD (OR = 0.42, 95% CI: 0.21 to 0.86, P = 0.0174)
Conclusions

The ideal pregnancy outcome can be achieved within 19mm from uterus fundus after single blastocyst transfer, The clinical pregnancy and live birth at a distance of more 19mm from the uterus fundus have a cliff-like downward trend.
Potentiating hypoxic microenvironment for antibiotic activation by photodynamic therapy to combat bacterial biofilm infections
Authors: Xiu Weijun , Wan Ling , Yang Kaili , Li Xiao , Yuwen Lihui , Dong Heng , Mou Yongbin , Yang Dongliang , Wang Lianhui 
Published: November 2021

Abstract
Traditional antibiotic treatment has limited efficacy for the drug-tolerant bacteria present in biofilms because of their unique metabolic conditions in the biofilm infection microenvironment. Modulating the biofilm infection microenvironment may influence the metabolic state of the bacteria and provide novel therapeutic routes. Here, photodynamic therapy (PDT)-activated chemotherapy by potentiating the hypoxia of biofilm infection microenvironment is proposed to tackle methicillin-resistant Staphylococcus aureus (MRSA) biofilm infections. In this study, PDT was used not only to eradicate MRSA biofilms in normoxic conditions, but also to potentiate the hypoxic microenvironment, which induces the anaerobic metabolism of MRSA and activates metronidazole to kill bacteria. Moreover, PDT-activated chemotherapy could polarize the macrophages to a M2-like phenotype and promote the repair of the biofilm infected wounds in mice. This biofilm infection microenvironment modulation strategy, whereby the hypoxic microenvironment is potentiated to synergize PDT with chemotherapy, provides an alternative pathway for efficient treatment of biofilm-associated infections.
Long Covid after Breakthrough COVID-19: the post-acute sequelae of breakthrough COVID-19
Authors: Al-Aly Ziyad , Bowe Benjamin , Xie Yan 
Published: November 2021

Abstract
The post-acute sequelae of COVID-19 have been described1, but whether breakthrough COVID-19 (that is the disease that ensues following vaccine breakthrough SARS-CoV-2 infection) results in post-acute sequelae is not yet clear. Here we use the national healthcare databases of the US Department of Veterans Affairs to characterize 6-month risks of incident post-acute sequelae in people with breakthrough COVID-19 who survived for at least 30 days after diagnosis. We show that compared to people with no evidence of COVID-19, beyond the first 30 days of illness, people with breakthrough COVID-19 exhibit a higher risk of death and broad array of incident post-acute sequelae in the pulmonary system, as well as extrapulmonary sequelae that include cardiovascular disorders, coagulation disorders, gastrointestinal disorders, general disorders (e.g., fatigue), kidney disorders, mental health disorders, metabolic disorders, musculoskeletal disorders, and neurologic disorders. Our analyses by care setting of the acute phase of the disease show that people who were not hospitalized during the first 30 days after diagnosis with breakthrough COVID-19 exhibit a small but not insignificant increase in risk of death and post-acute sequelae; the risks are further increased in people who were hospitalized during the acute phase of the disease. Our comparative approach shows that people with breakthrough COVID-19 exhibit lower risks of death and post-acute sequelae than people with COVID-19 who were not previously vaccinated for it; and in analyses among individuals who were hospitalized during the acute phase of the disease, people with breakthrough COVID-19 exhibit higher risks of death and post-acute sequelae than people with seasonal influenza. Altogether, our findings show increased risks of death and post-acute sequalae in people with breakthrough COVID-19; the risks are evident among those who were not hospitalized during the acute phase of the disease. Our comparative approach provides context for understanding the risks in relation to COVID-19 without prior vaccination and seasonal influenza. The findings will inform the ongoing effort to optimize strategies for prevention of breakthrough SARS-CoV-2 infections and will guide development and optimization of post-acute care pathways for people with breakthrough COVID-19.