bluuebunny/crossref_metadata_2025_split huggingface dataset:
Climate factors influencing effective use of geothermal resources in SE Poland: the Lublin trough
Authors: Ciapała Bartłomiej , Jurasz Jakub , Janowski Mirosław , Kępińska Beata 
Published: February 2021

AbstractAlthough geothermal resources are practically independent of climate factors, those factors significantly condition the potential use of the Earth’s natural heat resources. Unlike all the other factors limiting or facilitating the use of geothermal heat (like receivers’ temperature expectation, financial issues or local regulations), climate factors remain immovable. Thus, climate remains the main factor influencing the effective use of geothermal resources. Volumes of sold energy, typical capacity factors and rapid changes in heat demand may all influence the financial and technological performance of an investment. In the current paper, climate factors are translated into heat demand based on historical data (meteorological and district heating logs) by means of a dedicated artificial neural network, and analysed in terms of possible constraints and facilitators that might affect the effective use of geothermal energy. The results of ANN simulation indicate that average and typical operation is expected without any turbulences, yet about 10% of operating hours may require additional technical measures, like peak source support, smart management and buffers in order to limit pumping ramp rate. With appropriate dimensioning and exploitation, capacity factors as high as 60% are available, proving the potential for financially and environmentally effective use of geothermal resources.
Flipped learning design in EFL classrooms: implementing self-regulated learning strategies to develop language skills
Authors: Öztürk Mücahit , Çakıroğlu Ünal 
Published: February 2021

AbstractThis study examined the development of students’ language skills in a flipped English foreign language course designed with self-regulated learning strategies. The study was conducted with a quasi-experimental design. While the experimental group received the course through flipped classroom model designed with self-regulated learning strategies, control group did not use self-regulated learning strategies in the flipped classroom model. The results indicated that self-regulated learning strategies positively affected the development of foreign language skills in the flipped classroom model. A significant difference in favor of the group working with the platform including self-regulated learning strategies outperformed in speaking, reading, writing, and grammar test scores. However, no significant difference in the listening test results was found.
Criminal charge history, handgun purchasing, and demographic characteristics of legal handgun purchasers in California
Authors: Pear Veronica A. , Wright Mona A. , Shev Aaron B. , Wintemute Garen J. , Kagawa Rose M. C. 
Published: February 2021

Abstract
Background
The prevalence and characteristics of handgun purchasers’ criminal charge histories have never been described for a large population of firearm owners, but such information is critical to understanding risk factors for subsequent violence in this population. We sought to characterize legal handgun purchasers in California and compare this group to the state population, to quantify the proportion with a criminal charge history at purchase, and to identify modifiable factors associated with of having such a history.

Methods
This cross-sectional study of all 79,927 legal handgun purchasers aged 21–49 years in California in 2001 used log-linear generalized additive models to identify factors associated with having a criminal charge history at purchase. Subjects are from a longitudinal study of incident criminal activity among handgun purchasers.

Results
The majority (91.03%) of purchasers were male; whites were overrepresented and Hispanics were underrepresented relative to their population size. At the time of purchase, 16.68% had a criminal charge history and 10.71% had a criminal conviction. Among men with such a history, 31.28% had been charged with a violent crime and 16.54% had been charged with a firearm-related crime. The strongest factor associated with having a criminal charge history was redeeming a pawned handgun (prevalence ratio: 1.82; 95% confidence interval: 1.71, 1.93).

Conclusions
Despite California’s stringent firearm purchase laws, more than 1-in-6 handgun purchasers had a criminal charge history at purchase. This proportion may be higher in states with less restrictive firearm purchasing eligibility criteria.
Non-monotonic growth and motion of the South Atlantic Anomaly
Authors: Amit Hagay , Terra-Nova Filipe , Lézin Maxime , Trindade Ricardo I. 
Published: February 2021

AbstractThe South Atlantic Anomaly (SAA) is a region at Earth’s surface where the intensity of the magnetic field is particularly low. Accurate characterization of the SAA is important for both fundamental understanding of core dynamics and the geodynamo as well as societal issues such as the erosion of instruments at surface observatories and onboard spacecrafts. Here, we propose new measures to better characterize the SAA area and center, accounting for surface intensity changes outside the SAA region and shape anisotropy. Applying our characterization to a geomagnetic field model covering the historical era, we find that the SAA area and center are more time dependent, including episodes of steady area, eastward drift and rapid southward drift. We interpret these special events in terms of the secular variation of relevant large-scale geomagnetic flux patches on the core–mantle boundary. Our characterization may be used as a constraint on Earth-like numerical dynamo models.
Clinical outcome after knee ligament reconstruction with tendon allografts
Authors: Drogset Jon Olav , Størset Kristina Hovde , Nitteberg Thea Marie , Gifstad Tone 
Published: February 2021

AbstractPurposeThe purpose of this study is to investigate the clinical outcome for patients after knee ligament reconstructions with allografts at a university hospital.MethodsA total of 33 patients received allografts for reconstructive knee surgery between 2007 and 2017. The follow up evaluation consisted of a clinical knee examination including evaluation of range of motion (ROM), lateral and medial laxity, the Lachman test, the Pivot shift test, the sag test, the posterior drawer test and checking for patellofemoral pain. The following patient-reported outcome measures (PROMs) were used; the Lysholm Function Score, the Tegner activity score, and the Knee injury and Osteoarthritis Outcome Score (KOOS).ResultsTwenty-one (64%) patients were available for the follow-up evaluation and the mean follow-up time was 4.8 years. A total of 16 out of 21 patients had multiligament injuries of which the ACL was the ligament most frequently ruptured. At the time of follow-up, 14 out of 16 patients (87%) with ACL injury had Lachman test grade 0 or grade 1 + , and 12 out of 13 (92%) had a pivot shift grade 0 or 1 + . The mean Lysholm Score was 74. All mean KOOS subscale values were ≥ 59 at the follow-up. The preoperative Tegner activity score was 3 (range, 1–6) and 4 (range, 2–6) at follow up. There were no deep postoperative infections. A total of 19 out of 21 patients (90%) reported that they would have undergone surgery again had they known the clinical outcome in advance.ConclusionsThe patients improved from the preoperative score to the follow-up score in the knee-related Quality of Life (QoL) KOOS subscale. None of the patients were diagnosed with deep postoperative infections.
Computational design of highly stable and soluble alcohol dehydrogenase for NADPH regeneration
Authors: Xu Jinling , Zhou Haisheng , Yu Haoran , Deng Tong , Wang Ziyuan , Zhang Hongyu , Wu Jianping , Yang Lirong 
Published: February 2021

AbstractNicotinamide adenine dinucleotide phosphate (NADPH), as a well-known cofactor, is widely used in the most of enzymatic redox reactions, playing an important role in industrial catalysis. However, the absence of a comparable method for efficient NADP+ to NADPH cofactor regeneration radically impairs efficient green chemical synthesis. Alcohol dehydrogenase (ADH) enzymes, allowing the in situ regeneration of the redox cofactor NADPH with high specific activity and easy by-product separation process, are provided with great industrial application potential and research attention. Accordingly, herein a NADP+-specific ADH from Clostridium beijerinckii was selected to be engineered for cofactor recycle, using an automated algorithm named Protein Repair One-stop Shop (PROSS). The mutant CbADH-6M (S24P/G182A/G196A/H222D/S250E/S254R) exhibited a favorable soluble and highly active expression with an activity of 46.3 U/mL, which was 16 times higher than the wild type (2.9 U/mL), and a more stable protein conformation with an enhanced thermal stability: Δ $${T}_{1/2}^{60\mathrm{min}}$$

T

1
/
2

60
min

=  + 3.6 °C (temperature of 50% inactivation after incubation for 60 min). Furthermore, the activity of CbADH-6M was up-graded to 2401.8 U/mL by high cell density fermentation strategy using recombinant Escherichia coli, demonstrating its industrial potential. Finally, the superb efficiency for NADPH regeneration of the mutant enzyme was testified in the synthesis of some fine chiral aromatic alcohols coupling with another ADH from Lactobacillus kefir (LkADH).
Redirection of metabolic flux in Shewanella oneidensis MR-1 by CRISPRi and modular design for 5-aminolevulinic acid production
Authors: Yi Ying-Chen , Ng I-Son 
Published: February 2021

AbstractProgramming non-canonical organisms is more attractive due to the prospect of high-value chemical production. Among all,Shewanella oneidensisMR-1 possesses outstanding heme synthesis ability and is well-known for electron transfer, thus has high potential in microbial fuel cell and bioremediation. However, heme, as the final product of C4 and C5 pathways, is regulated by heme cluster for the high-value 5-aminolevulinic acid (ALA) for cancer photodynamic therapy, which has never been explored in MR-1. Herein, the heme metabolism in MR-1 was firstly optimized for ALA production. We applied CRISPR interference (CRISPRi) targeted on the genes to fine-tune carbon flux in TCA cycle and redirected the carbon out-flux from heme, leading to a significant change in the amino acid profiles, while downregulation of the essentialhemB showed a 2-fold increasing ALA production via the C5 pathway. In contrast, the modular design including of glucokinase, GroELS chaperone, and ALA synthase fromRhodobacter capsulatusenhanced ALA production markedly in the C4 pathway. By integrating gene cluster under dual T7 promoters, we obtained a new strain M::TRG, which significantly improved ALA production by 145-fold. We rewired the metabolic flux of MR-1 through this modular design and successfully produced the high-value ALA compound at the first time.
A CT-based radiomics nomogram for distinguishing between benign and malignant bone tumours
Authors: Sun Weikai , Liu Shunli , Guo Jia , Liu Song , Hao Dapeng , Hou Feng , Wang Hexiang , Xu Wenjian 
Published: February 2021

AbstractBackgroundWe sought to evaluate the performance of a computed tomography (CT)-based radiomics nomogram we devised in distinguishing benign from malignant bone tumours.MethodsTwo hundred and six patients with bone tumours were spilt into two groups: a training set (n = 155) and a validation set (n = 51). A feature extraction process based on 3D Slicer software was used to extract the radiomics features from unenhanced CT images, and least absolute shrinkage and selection operator logistic regression was used to calculate the radiomic score to generate a radiomics signature. A clinical model comprised demographics and CT features. A radiomics nomogram combined with the clinical model and the radiomics signature was constructed. The performance of the three models was comprehensively evaluated from three aspects: identification ability, accuracy, and clinical value, allowing for generation of an optimal prediction model.ResultsThe radiomics nomogram comprised clinical and radiomics signature features. The nomogram model displayed good performance in training and validation sets with areas under the curve of 0.917 and 0.823, respectively. The areas under the curve, decision curve analysis, and net reclassification improvement showed that the radiomics nomogram model could obtain better diagnostic performance than the clinical model and achieve greater clinical net benefits than the clinical and radiomics signature models alone.ConclusionsWe constructed a combined nomogram comprising a clinical model and radiomics signature as a noninvasive preoperative prediction method to distinguish between benign and malignant bone tumours and assist treatment planning.
Multisensor robust localization in various environment with correlation checking test
Authors: Ohashi Nozomu , Funabora Yuki , Doki Shinji , Doki Kae 
Published: February 2021

AbstractProbabilistic localization based on Bayesian theory has been researched as a sensor fusion method to improve the robustness of localization. Pieces of position information, generated by sensors’ observation models with consideration for noises, are fused according to Bayesian theory. However, having large noises not considered in their observation models, the sensors output erroneous position information; thus, the fusion result has a significant error, even when the other sensors output correct ones. In this research, we have proposed a sensor fusion system with a relative correlation checking test to realize robust localization. Pieces of erroneous position information, biased against others and having a negative correlation with others, are detected and excluded in our proposed system by checking their correlation between all of them. The purpose of this paper is to evaluate the robustness of our fusion system by conducting recursive localization experiments in various environments.
Use of population input functions for reduced scan duration whole-body Patlak 18F-FDG PET imaging
Authors: van Sluis Joyce , Yaqub Maqsood , Brouwers Adrienne H. , Dierckx Rudi A. J. O. , Noordzij Walter , Boellaard Ronald 
Published: February 2021

Abstract
Whole-body Patlak images can be obtained from an acquisition of first 6 min of dynamic imaging over the heart to obtain the arterial input function (IF), followed by multiple whole-body sweeps up to 60 min pi. The use of a population-averaged IF (PIF) could exclude the first dynamic scan and minimize whole-body sweeps to 30–60 min pi. Here, the effects of (incorrect) PIFs on the accuracy of the proposed Patlak method were assessed. In addition, the extent of mitigating these biases through rescaling of the PIF to image-derived IF values at 30–60 min pi was evaluated.

Methods
Using a representative IF and rate constants from the literature, various tumour time-activity curves (TACs) were simulated. Variations included multiplication of the IF with a positive and negative gradual linear bias over 60 min of 5, 10, 15, 20, and 25% (generating TACs using an IF different from the PIF); use of rate constants (K1, k3, and both K1 and k2) multiplied by 2, 1.5, and 0.75; and addition of noise (μ = 0 and σ = 5, 10 and 15%). Subsequent Patlak analysis using the original IF (representing the PIF) was used to obtain the influx constant (Ki) for the differently simulated TACs. Next, the PIF was scaled towards the (simulated) IF value using the 30–60-min pi time interval, simulating scaling of the PIF to image-derived values. Influence of variabilities in IF and rate constants, and rescaling the PIF on bias in Ki was evaluated.

Results
Percentage bias in Ki observed using simulated modified IFs varied from − 16 to 16% depending on the simulated amplitude and direction of the IF modifications. Subsequent scaling of the PIF reduced these Ki biases in most cases (287 out of 290) to < 5%.

Conclusions
Simulations suggest that scaling of a (possibly incorrect) PIF to IF values seen in whole-body dynamic imaging from 30 to 60 min pi can provide accurate Ki estimates. Consequently, dynamic Patlak imaging protocols may be performed for 30–60 min pi making whole-body Patlak imaging clinically feasible.
White oak (Quercus fabri Hance) regenerated stump sprouts show few senescence symptoms during 40 years of growth in a natural forest
Authors: Sun Honggang , Wu Sisheng , Wu Liwen 
Published: February 2021

Abstract
Background
The relationship between physiological age of parental trees and lifespan of clonal offspring is unclear. White oak (Quercus fabri Hance) has a high sprouting capability after harvest, with the regenerated sprouts being typical clonal individuals. To determine whether regenerated sprouts undergo rapid senescence compared with the parent, the senescence levels of 5-, 10-, 20- and 40-year-old regenerated stump sprouts in a natural forest were evaluated. The antioxidative abilities and transcriptomes in these leaves and shoots were compared.

Results
Older regenerated sprouts still had robust antioxidative systems, with 40-year-old sprouts having lower peroxidation product levels but similar antioxidative enzyme activity levels compared with 5-year-old sprouts. Older leaves had greater transcriptional activities in pathways related to cell growth and division than younger leaves. However, older sprouts had some unhealthy characteristics, such as increased base excision repair levels and upregulated phagosome, proteasome and glycerophospholipid metabolism pathways in 40-year-old leaves, which indicates that DNA damage and tissue remodeling occurred more frequently than in younger leaves. Additionally, plant-pathogen interactions and MAPK signals pathways were upregulated in older shoots, which indicates that older shoots suffered from more pathogen-related biotic stress.

Conclusions
The 40-year-old sprouts still had the same vitality level as the 5-year-old sprouts, although the former had some unhealthy characteristics. We conclude that during their first 40 years of growth, regenerated stump sprouts do not begin to senesce, and that physiological age of parental trees does not significantly affect the lifespan of its clonal offspring.
Hematopoietic stem cell transplantation for systemic sclerosis: Brazilian experience
Authors: Henrique-Neto Álvaro , Vasconcelos Marianna Yumi Kawashima , Dias Juliana Bernardes Elias , de Moraes Daniela Aparecida , Gonçalves Maynara Santana , Zanin-Silva Djúlio César , Zucoloto Talita Graminha , de Oliveira Marília de Fátima Cirioli , Dotoli Giuliana Martinelli , Weffort Luiz Fernando , Leopoldo Vanessa Cristina , Oliveira Maria Carolina 
Published: February 2021

AbstractBackgroundIn the past 20 years, hematopoietic stem cell transplantation (HSCT) has been investigated as treatment for systemic sclerosis (SSc). The goal of HSCT is to eradicate the autoreactive immune system, which is replaced by a new immune repertoire with long-lasting regulation and tolerance to autoantigens. Here, we describe the clinical outcomes of severe and refractory SSc patients that underwent HSCT at a single Brazilian center.Patients and methodsThis is a longitudinal and retrospective study, including 70 adult SSc patients, with an established diagnosis of SSc, and who underwent autologous HSCT from 2009 to 2016. The procedure included harvesting and cryopreservation of autologous hematopoietic progenitor cells, followed by administration of an immunoablative regimen and subsequent infusion of the previously collected cells. Patients were evaluated immediately before transplantation, at 6 months and then yearly until at least 5-years of post-transplantation follow-up. At each evaluation time point, patients underwent clinical examination, including modified Rodnan’s skin score (mRSS) assessment, echocardiography, high-resolution computed tomography of the lungs and pulmonary function.ResultsMedian (range) age was 35.9 (19–59), with 57 (81.4%) female and median (range) non-Raynaud’s disease duration of 2 (1–7) years. Before transplantation, 96% of the patients had diffuse skin involvement, 84.2%, interstitial lung disease and 67%, positive anti-topoisomerase I antibodies. Skin involvement significantly improved, with a decline in mRSS at all post-transplantation time points until at least 5-years of follow-up. When patients with pre-HSCT interstitial lung disease were analyzed, there was an improvement in pulmonary function (forced vital capacity and diffusing capacity of lung for carbon monoxide) over the 5-year follow-up. Overall survival was 81% and progression-free survival was 70.5% at 8-years after HSCT. Three patients died due to transplant-related toxicity, 9 patients died over follow-up due to disease reactivation and one patient died due to thrombotic thrombocytopenic purpura.ConclusionsAutologous hematopoietic progenitor cell transplantation improves skin and interstitial lung involvement. These results are in line with the international experience and support HSCT as a viable therapeutic alternative for patients with severe and progressive systemic sclerosis.
Relation between grades of acquired nasopharyngeal stenosis and severity of obstructive sleep apnea symptoms
Authors: El-Anwar Mohammad Waheed , Hendawy Ehsan , Eesa Mohamed 
Published: February 2021

Abstract
Background
Nasopharyngeal stenosis (NPS) is a rare condition defined as the obstruction of the communication between the oropharynx and nasopharynx owing to scar contracture of the soft palate, tonsillar pillars, and posterior pharyngeal wall. NPS could be primary (attributed to a disease process such as rhinoscleroma) or secondary caused by prior surgery. In this study, we discuss the clinical manifestation of the acquired nasopharyngeal stenosis (NPS) and to investigate if there is a relation between the grade of the acquired NPS and the severity of obstructive sleep apnea (OSA) and patients’ symptoms. So, this cross-sectional study was conducted on patients who had post-surgical NPS of different grades. Then, the severity of snoring ± OSA and VAS of symptoms in those patients was assessed in different grades of the NPS, and the results were also statistically compared.

Results
Within the included 22 patients, there were no statistical differences between grade 1 and in grade 2 as regards age (p = 0.0619) or sex (p = 0.21137). The mean AHI was significantly more (p = 0.004) in grade 2 NPS than grade 1 NPS. The VAS of difficult nasal breathing and dysphagia were significantly worse (p < 0.0001) in grade 2 NPS than in grade 1, while the VAS of snoring did not differ significantly (p = 0.3466) between grade 1 and grade 2 NPS.

Conclusion
Grade 2 NPS leads to significantly more severe AHI, dysphagia, and difficult nasal breathing than grade 1 NPS. But the difference in the grade of NPS did not cause differences in the snoring intensity.
Effect of bonding or unbonding on seismic behavior of bridge elastomeric bearings: lessons learned from past earthquakes in China and Japan and inspirations for future design
Authors: Xiang Nailiang , Goto Yoshiaki , Alam M. Shahria , Li Jianzhong 
Published: February 2021

AbstractEconomical laminated elastomeric bearings are well-adopted options for load transmission components of bridges from superstructure to substructure. In most cases, the design of such elastomeric bearings primarily depends on the requirements from service-level conditions such as superstructure thermal movements, with little consideration of extreme loads like earthquakes. However, bridge elastomeric bearings are very likely to be subjected to earthquake hazards, particularly for bridges located in high seismic regions. This study presents an overview of the observed typical damages of bridge elastomeric bearings in the past major earthquakes mainly in China and Japan. Comparisons of different damage patterns are conducted based on the different installations of bridge elastomeric bearings (bonding or un-bonding). The effect of bearing installation methods on the overall seismic behavior of bridges is also discussed. A desirable installation method for bridge elastomeric bearings against strong earthquake loads is recommended, which is expected to overcome the critical limitations of current design practice.