bluuebunny/crossref_metadata_2025_split huggingface dataset:
The potential risk of rabies transmission in Madagascar- a report on the canine vaccination coverage in an urban district of Mahajanga
Authors: Rajoromanana Blaise , Edosoa Glenn T. , Andrianasolo Radonirina L. , Rietmann Sylvie , Nyirenda Gabriel , Marks Florian , Rakotozandrindrainy Raphaël , Haselbeck Andrea , Farasolo Paule-Aimée Ralison 
Published: August 2021

Abstract
BackgroundThe Rabies lyssavirus is one of 16 species of the Lyssavirus genus causing fatal encephalomyelitis in humans. Infection usually occurs after a bite by an infected mammal. More than 99% of human rabies deaths worldwide are caused by a canine rabies with children at highest risk of infection. Although rabies has been endemic in the region of Mahajanga in Madagascar for a long time, research evaluating the current situation is still scarce. Here, we conducted a cross-sectional study to describe the dog demography and a survey of dog owners to evaluate rabies vaccination coverage, disease knowledge and measures taken in the districts against rabies in order to inform future interventions in the urban district of Mahajanga in the North West of Madagascar.ResultsA total of 400 dogs were recorded, of which 338 (84.5%) were owned. The vaccine coverage against rabies was at 34.4% (116/338). The reported key barriers to vaccination were lack of financial resources and the lack of geographic access to veterinary services. The transmission route of rabies being dog bites was known to 85.4% of the surveyed dog owners (105/123). Out of 19 reported dog bites, 13 were caused by the owner`s or the neighbour’s dog. 6 out of 19 cases affected children between 7 and 15 years of age. ConclusionMost of the dogs in the urban district of Mahajanga are accessible for preventive vaccination through their owners, but less than one third of the canine population is vaccinated against rabies. Assuming that stray dogs are not vaccinated against rabies, Mahajanga is far from reaching the 70% coverage believed to minimise the spread of the disease. The prioritization of dog owner education, school campaigns that sensitize children on the interactions with dogs and vaccination campaigns for owned dogs in collaboration with local veterinary services has shown success in other settings in Africa and will be crucial for future rabies eradication in Madagascar.
Hypothermia and rewarming prior to reperfusion, prevents intracranial pressure elevation after ischaemic stroke in rats: an investigation to define the importance of cooling during reperfusion
Authors: Omileke Daniel , Azarpeykan Sara , Bothwell Steven W , Pepperall Debbie , Beard Daniel J , Coupland Kirsten , Patabendige Adjanie , Spratt Neil J 
Published: August 2021

Abstract
Reperfusion therapies re-establish blood flow after arterial occlusion and improve outcome for ischaemic stroke patients. Intracranial pressure (ICP) elevation occurs 18–24 h after experimental stroke. This elevation is prevented by short-duration hypothermia spanning the time of reperfusion. We aimed to determine whether hypothermia-rewarming completed prior to reperfusion, also prevents ICP elevation 24 h post-stroke. Transient middle cerebral artery occlusion was performed on male outbred Wistar rats. Sixty-minute hypothermia to 33℃, followed by rewarming was induced prior to reperfusion in one group, and after reperfusion in another group. Normothermia controls received identical anaesthesia protocols. ΔICP from pre-stroke to 24 h post-stroke was measured, and infarct volumes were calculated. Rewarming pre-reperfusion prevented ICP elevation (ΔICP = 0.3 ± 3.9 mmHg vs. normothermia ΔICP = 5.2 ± 2.1 mmHg, p = 0.02) and reduced infarct volume (pre-reperfusion = 78.6 ± 23.7 mm3 vs. normothermia = 125.1 ± 44.3 mm3, p = 0.04) 24 h post-stroke. There were no significant differences in ΔICP or infarct volumes between hypothermia groups rewarmed pre-or post-reperfusion. Hypothermia during reperfusion is not necessary for prevention of ICP rise or infarct volume reduction. Short-duration hypothermia is a broadly applicable potential early treatment strategy for stroke patients prior to- during-, and after reperfusion therapy.
Assessing the Blockage of Toxicity of Stereolithographically 3D-Printed Parts Coated with Parylene C
Authors: Huang Kaiyuan 
Published: August 2021

Abstract
Comparing to fused deposition modeling (FDM), the stereolithography (SLA) 3D printing method can provide higher fabrication speed and much better resolution, which is demanded by biomedical usage. However, the additives migrating from SLA printed plastic are toxic to creatures, and the method to reduce the toxicity by soaking and exposure to ultraviolet is inadequate, while current biocompatible photo-polymerizing resins are rare and expensive. Here, we provide a method to nearly block all toxicity by coating the fabrication with parylene C. We use early life zebrafish to assess the toxicity of the SLA printed petri dishes whether or not coated with parylene C. The result shows nearly no influence of the coated petri dishes cultured early life zebrafish development compared to control groups, while the fertilized embryos of uncoated groups get killed in 24 hours. The method provides a cheap and easy way to produce customized biocompatible fabrication, which contributes to the popularization of 3D printed products, either in daily life or in research.
Altered circulating Tregs and their functional cytokines in hypertensive patients with or without left ventricular hypertrophy
Authors: Tang Ying , Shen Li , Xu Danyan , Bao Jing-hui 
Published: August 2021

Abstract
Background:Left ventricular hypertrophy (LVH) is the most common target organ damage in hypertension. In addition to the increased left ventricular afterload, immune injury participates in LVH pathogenesis. CD4+CD25+Foxp3+ regulatory T lymphocytes (Tregs) are a T cell subset with immunomodulatory effects; abnormal Treg numbers or functions can cause immune disorders. This study aimed to explore the role of Tregs in LVH by investigating circulating Tregs and associated cytokine levels in hypertensive patients with LVH.Methods:Blood samples were collected from 69 healthy individuals (control group, CG), 91 hypertensive patients with LVH (LVH group) and 83 hypertensive patients without LVH (essential hypertension, EH group). Circulating Tregs and associated cytokines were measured by flow cytometry and enzyme-linked immunosorbent assays, respectively.Results:Circulating Tregs were significantly lower in EH and LVH patients than in CG subjects. The circulating Treg level was lower in LVH patients than in EH patients. No correlation between blood pressure regulation and Treg levels was found in EH or LVH patients. Furthermore, circulating Tregs in postmenopausal females were lower than those in males among LVH patients. Additionally, serum IL-10 and transforming growth factor (TGF)-β1 were decreased in EH and LVH patients, and the serum IL-6 level was significantly increased in LVH patients. Circulating Tregs were negatively correlated with CK, LDL, apoB, high-sensitivity C-reactive protein and left ventricular mass index values.Conclusion:Our study is the first to demonstrate the significantly decreased circulating Treg proportion in LVH patients. The protective effect of Tregs in LVH is independent of blood pressure regulation. The functional Treg cytokines IL-6, IL-10 and TGF-β1 participate in this immunomodulatory process.Trial registration: The study was approved by the Institutional Medical Ethics Committee of the Second Xiangya Hospital of Central South University (2018/No.046).
Anxiety disorders in patients with noncardiac chest pain: association with health-related quality of life and chest pain severity
Authors: Hamel Stéphanie , Denis Isabelle , Turcotte Stéphane , Fleet Richard , Archambault Patrick , Dionne Clermont , Foldes-Busque Guillaume 
Published: August 2021

Abstract
BackgroundPatients with noncardiac chest pain (NCCP) report more severe symptoms and lowered health-related quality of life when they present with comorbid panic disorder (PD). Although generalized anxiety disorder (GAD) is the second most common psychiatric disorder in these patients, its impact on NCCP and health-related quality of life remains understudied. This study describes and prospectively compares patients with NCCP with or without PD or GAD in terms of (1) NCCP severity; and (2) the physical and mental components of health-related quality of life. MethodsA total of 915 patients with NCCP were consecutively recruited in two emergency departments. The presence of comorbid PD or GAD was assessed at baseline with the Anxiety Disorder Schedule for DSM-IV. NCCP severity at baseline and at the six-month followup was assessed with a structured telephone interview, and the patients completed the 12-item Short-Form Health Survey Version 2 (SF-12v2) to assess health-related quality of life at both time points. ResultsAverage NCCP severity decreased between baseline and the six-month follow-up ( p <.001) and was higher in the patients with comorbid PD or GAD ( p <.001) at both time points compared to those with NCCP only. However, average NCCP severity did not differ between patients with PD and those with GAD ( p = 0.901). The physical component of quality of life improved over time ( p = 0.016) and was significantly lower in the subset of patients with PD with or without comorbid GAD compared to the other groups ( p <.001). A significant time x group interaction was found for the mental component of quality of life ( p = 0.0499). GAD with or without comorbid PD was associated with a lower mental quality of life, and this effect increased at the six-month follow-up. ConclusionsComorbid PD or GAD are prospectively associated with increased chest pain severity and lowered health-related quality of life in patients with NCCP. PD appears to be mainly associated with the physical component of quality of life, while GAD has a greater association with the mental component. Knowledge of these differences could help in the management of patients with NCCP and these comorbidities.
Predictors for development of oxaliplatin-induced peripheral neuropathy in cancer patients as determined by ordered logistic regression analysis
Authors: Kanbayashi Yuko , Ishikawa Takeshi , Kuriu Yoshiaki , Otsuji Eigo , Takayama Koichi 
Published: August 2021

Abstract
This retrospective study aimed to identify predictors for the development of oxaliplatin-induced peripheral neuropathy (OXAIPN). Between January 2017 and March 2021, a total 322 cancer patients at our hospital who were receiving oxaliplatin were enrolled. For the regression analysis of factors associated with oxaliplatin-induced peripheral neuropathy, variables were extracted manually from medical charts. The level of OXAIPN was evaluated using the National Cancer Institute’s Common Terminology Criteria for Adverse Events (version 5). Multivariate ordered logistic regression analysis was performed to identify predictors for the development of OXAIPN. Optimal cut-off thresholds were determined using receiver operating characteristic (ROC) analysis. Values of P < 0.05 (2-tailed) were considered significant. Significant factors identified included body mass index (BMI) (odds ratio [OR] = 1.06, 95% confidence interval [CI] = 1.00–1.12; P = 0.046), number of cycles (OR = 1.09, 95%CI = 1.05–1.14; P < 0.0001), S-1 plus oxaliplatin (SOX) regimen (OR = 0.54, 95%CI = 0.32–0.92; P = 0.023), concomitant use of proton pump inhibitors (PPIs) (OR = 1.64, 95%CI = 1.05–2.58; P = 0.031) and concomitant use of analgesic adjuvant (OR = 3.30, 95%CI = 1.09–9.97; P = 0.035). ROC analysis revealed that peripheral neuropathy (grade 2 or higher) was more likely to occur with ≥8 cycles, with 66.3% sensitivity and 56.5% specificity (area under the curve [AUC] = 0.65), and BMI ≥20.5 kg/m2, with 63.3% sensitivity and 47.8% specificity (AUC = 0.54). In conclusion, BMI, number of cycles, SOX regimen, concomitant use of PPIs and concomitant use of analgesic drugs were identified as significant predictors for the development of OXAIPN.
GSK-126 protects CA1 neurons from H3K27me3-mediated apoptosis in cerebral ischemia
Authors: Wang Zhongcheng , Su Yaxin , Zhang Lei , Lan Ting , Li Li , Qi Suhua 
Published: August 2021

Abstract
Epigenetics, including histone modifications, play a significant role in central nervous system diseases, but the underlying mechanism remains to be elucidated. The aim of this study was to evaluate the role of H3K27me3 in regulating transcriptomic and pathogenic mechanisms following global ischemic stroke. Here, we found that in vivo ischemic/reperfusion (I/R) injury induced marked upregulation of H3K27me3 in the hippocampus. The administration of GSK-126 to rat brains decreased the levels of H3K27me3 in the hippocampus and reduced neuronal apoptosis after experimental stroke. Furthermore, ChIP-seq data demonstrated that the primary role of GSK-126 in the ischemic brain is to reduce H3K27me3 enrichment, mediating negative regulation of the execution phase of apoptosis and the MAPK signaling pathway. Further study suggested that the protective role of GSK-126 in ischemic rats was antagonized by U0126, an inhibitor of ERK1/2. Collectively, we demonstrated the potential of H3K27me3 as a novel stroke therapeutic target, and GSK-126 exerted a neuroprotective function in ischemic brain injury, which might be associated with activation of the MAPK/ERK pathway.
Public health vulnerability due to the exposure of dissolved metal(oid)s in tap water from a mega city (Dhaka, Bangladesh)
Authors: Hossain Shabiha , Anik Amit Hasan , Khan Rahat , Ahmed Farah Tasneem , Siddique Md. Abu Bakar , Khan Abdul Hadi Al Nafi , Saha Narottam , Idris Abubakr M. , Alam Mahbub 
Published: August 2021

Abstract
Tap water is the sole source of water in urban cities but gradually being polluted by potentially toxic elements due to unplanned urbanization. The south Asian megacity, Dhaka (capital of Bangladesh) encounters drastic water pollution resulting mainly from anthropogenic differential factors. Consumption of such polluted water distributed to residents by pipelines can trigger health risks. Therefore, this study investigated the public health vulnerability associated with dissolved metal(oid)s in tap water collected across Dhaka city. The concentrations of Cr, Mn, Fe, Co, Ni, Cu, Zn, As, Cd, Hg and Pb in measured tap water ranged from 8-156, 7–73, 400-20100, 12–110, 7-101, 12–136, 12–908, 0.03–9.75, 1–5, 0.22–1.30, and 8-118 µg/L, respectively. Among the observed elements, Pb, Cr, and Fe concentrations in 18%, 26%, and 75% of sampling sites, respectively, exceeded the standard guideline criteria of Bangladesh. Entropy-based water quality index demonstrated that ~ 12% of sampling sites possessed water unsuitable for drinking and other household works. The dominant sources of water pollution in this region are industrial effluents and domestic sewage. Both non-carcinogenic and carcinogenic health risks are invoked mostly from ingestion of Co and Pb. Regular consumption of this tap water without further pretreatment may result in detrimental health consequences to both children and adults due to the physiological accumulation of toxic elements over time. This study highlighted a comprehensive scenario of the potentially toxic elements in the tap water of Dhaka city, which will allow policymakers to take adequate measures for sustainable water quality management.
Gut microbiome composition, not alpha diversity, is associated with survival in a natural vertebrate population
Authors: Worsley Sarah F , Davies Charli S , Mannarelli Maria-Elena , Hutchings Matthew I , Komdeur Jan , Burke Terry , Dugdale Hannah L , Richardson David S 
Published: August 2021

Abstract
Background: The vertebrate gut microbiome (GM) can vary substantially across individuals within the same natural population. Although there is evidence linking the GM to health in captive animals, very little is known about the consequences of GM variation for host fitness in the wild. Here, we explore the relationship between faecal microbiome diversity, body condition and survival using data from the long-term study of a discrete natural population of the Seychelles warbler ( Acrocephalus sechellensis ) on Cousin Island. To our knowledge, this is the first time that GM differences associated with survival have been fully characterised for a natural vertebrate species, across multiple age groups and breeding seasons. Results: We identified substantial variation in GM community structure among sampled individuals, which was partially explained by breeding season (7% of the variance), and host age class (up to 1% of the variance). We also identified significant differences in GM community membership between individuals that survived, versus those that had died by the following breeding season. Individuals that died carried reduced abundances of beneficial taxa in the bacterial order Clostridiales , but increased abundances of taxa that are known to be opportunistic pathogens (e.g. members of the Chloroflexi and Propionibacteriales ). However, there was no association between GM alpha diversity (the diversity of bacterial taxa within a sample) and survival to the next breeding season, or with individual body condition. Additionally, we found no association between GM community membership and individual body condition. Conclusions: These results demonstrate that components of the vertebrate GM can be associated with host fitness in the wild, although whether changes in bacterial abundance contribute to, or are only correlated with, the differential survival observed remains unclear. Importantly, it suggests that components of the GM may be under selection, and, thus, could have the potential to influence the evolution of host species living in natural populations.
A reduced level of the long non-coding RNA SNHG8 activates the NF-kappaB pathway by releasing functional HIF-1alpha in a hypoxic inflammatory microenvironment
Authors: Wang Chenxin , Yang Qiaolin , Han Yineng , Liu Hao , Wang Yue , Huang Yiping , Zheng Yunfei , Li Weiran 
Published: August 2021

Abstract
Background

A series of biochemical responses, including hypoxia and aseptic inflammation, occur in periodontal ligament cells (PDLCs) during periodontal tissue remodeling of orthodontic tooth movement (OTM). However, the role of long non-coding RNA (lncRNA) in these responses is still largely unknown. We investigated the role of the lncRNA SNHG8 in hypoxic and inflammatory responses during OTM, and explored the underlying mechanisms.
Methods

The expression pattern of SNHG8, and hypoxic and inflammatory responses under compressive force, were analyzed by qRT-PCR, immunohistochemistry, and western blotting, in vivo and in vitro. The effect of overexpression or knockdown of SNHG8 on the nuclear factor-kappaB (NF-κB) pathway was evaluated. RNA sequencing was performed for mechanistic analysis. The interaction between SNHG8 and hypoxia-inducible factor (HIF)-1α was studied using catRAPID, RNA immunoprecipitation, and RNA pulldown assays. The effect of the SNHG8–HIF-1α interaction on the NF-κB pathway was determined by western blotting.
Results

The NF-κB pathway was activated, and HIF-1α release was stabilized, in PDLCs under compressive force as well as in OTM model rats. The SNHG8 level markedly decreased both in vivo and in vitro. Overexpression of SNHG8 decreased the expression levels of inflammatory cytokines, the phosphorylation of p65, and the degradation of IκBα in PDLCs, whereas knockdown of SNHG8 reversed these effects. Mechanically, RNA sequencing showed that differentially expressed genes were enriched in cellular response to hypoxia after SNHG8 overexpression. SNHG8 binds to HIF-1α, thus preventing HIF-1 from activating downstream genes, including those related to the NF-κB pathway.
Conclusion

SNHG8 binds to HIF-1α. During OTM, the expression of SNHG8 dramatically decreased, releasing free functional HIF-1α and activating the downstream NF-κB pathway. These data suggest a novel lncRNA-regulated mechanism during periodontal tissue remodeling in OTM.
Multi Expression Programming
Authors: Oltean Mihai , Dumitrescu D. 
Published: August 2021

Abstract
Multi Expression Programming (MEP) is a new evolutionary paradigm intended for solving computationally difficult problems. MEP individuals are linear entities that encode complex computer programs. MEP chromosomes are represented in the same way as C or Pascal compilers translate mathematical expressions into machine code. MEP is used for solving some difficult problems like symbolic regression and game strategy discovering. MEP is compared with Gene Expression Programming (GEP) and Cartesian Genetic Programming (CGP) by using several well-known test problems. For the considered problems MEP outperforms GEP and CGP. For these examples MEP is two magnitude orders better than CGP.
Characterization of genomic, physiological, and probiotic features of Lactiplantibacillus plantarum DY46 strain isolated from Turkish fermented turnip juice (Shalgam)
Authors: Yetiman Ahmet E. , KESKİN Abdullah , DARENDELI Busra Nur , KOTIL Seyfullah Enes , ORTAKCI Fatih , DOGAN Mahmut 
Published: August 2021

Abstract
A new Lb. plantarum strain DY46 was isolated from a traditionally fermented non-alcoholic beverage called shalgam from the Southern region of Anatolia following incubation on MRS agar at 30°C for 5 days. DY46 is gram-positive, short rod and catalase-negative. This bacterium fermented 22 of the 49 substrates tested on API CH50 fermentation panels. Whole-genome sequencing was performed using the Illumina Miseq platform to learn more about the metabolic capabilities of DY46. The sequences were assembled into a 3.32 Mb draft genome using PATRIC 3.6.8. consisting of 153 contigs, and preliminary genome annotation was performed using the RAST algorithm. The DY46 genome consists of a single circular chromosome of 3,332,827 bp that is predicted to carry 3219 genes, including 61 tRNA genes, 2 rRNA operons. The genome has a GC content of 44.3% includes 98 predicted pseudogenes, 25 complete or partial transposases and 3 intact prophages. DY46 genome also predicted to carry genes of Plantaricin-E, Plantaricin-F and Plantaricin-K showing the antimicrobial potential of this bacterium which can be linked-to in vitro antagonism tests that DY46 can inhibit Salmonella Typimirium ATCC14028, Klebsiella pneumonie ATCC13883, and Proteus vulgaris ATCC8427. The acid and bile tolerance of DY46 revealed this strain could potentially pass through the stomach and reach into the gut to provide probiotic therapeutic affects on health.