bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effects of Dexmedetomidine Administration on Outcomes in Critically Ill Patients with Acute Kidney Injury: A Propensity Score-Matching Analysis
Authors: Yang Aixiang , Yang Jing , Zhou Biying , Qian Jinxian , Jiang Liyang , Jiang Zhuo , Lu Guoyuan 
Published: June 2021

Abstract
Background

Dexmedetomidine (DEX) had organ protection effects and could decrease mortality in animal models, but its association with mortality and length of stay (LOS) in ICU and hospital in critically ill patients was conflicting. Whether acute kidney injury (AKI) subgroup of critically ill patients could benefit from DEX was unknown. The present study aimed to evaluate the effects of DEX on clinical outcomes of critically ill patients with AKI.
Methods

Data were extracted from the Medical Information Mart for Intensive Care Ⅲ database (MIMIC Ⅲ). Propensity score matching (PSM) analysis (1:3), cox proportional hazards model, linear regression and logistic regression model were used to assess the effect of DEX on clinical outcomes.
Results

After PSM, 324 pairs of patients were matched between the patients with DEX administration and those without. DEX administration was associated with decreased in-hospital mortality [hazard ratio (HR) 0.287; 95% CI 0.151–0.542; P < 0.001] and 90-day mortality [HR 0.344; 95% CI 0.221–0.534; P < 0.001], and it was also associated with reduced length of stay (LOS) in ICU [4.54(3.13,7.72) versus 5.24(3.15,10.91), P < 0.001] and LOS in hospital [11.63(8.02,16.79) versus 12.09(7.83,20.44), P = 0.002]. Subgroup analysis showed the above associations existed only in mild and moderate AKI subgroups, but not in severe AKI subgroup. Nevertheless, DEX administration was not associated with the recovery of renal function [HR 1.199; 95% CI 0.851–1.688; P = 0.300].
Conclusions

DEX administration improved outcomes in critically ill patients with mild and moderate AKI and could be a good choice of sedation.
RaDiCo, the French National Program on Rare Disease Cohorts
Authors: Amselem Serge , Gueguen Sonia , Weinbach Jérôme , Clement Annick , Landais Paul 
Published: June 2021

Abstract
Background: Rare diseases (RDs) affect nearly 3 million people in France and at least 26-30 million people in Europe. These diseases, which represent a major medical concern, are mainly of genetic origin, often chronic, progressive, degenerative, life threatening and disabling, accounting for more than one third of all deaths occurring during infancy. In this context, there are needs for coordinated information on RDs at national /international levels, based on high quality, interoperable and sharable data. The main objective of the RaDiCo (Rare Disease Cohorts) program, coordinated by Inserm, was the development of RD e-cohorts via a national platform. The cohort projects were selected through a national call in 2014. The e-cohorts are supported by an interoperable platform, equivalent to an infrastructure, constructed on the "cloud computing" principle and in compliance with the European General Data Protection Regulation. It is dedicated to allow a continuous monitoring of data quality and consistency, in line with the French Health Data Hub. Results: Depending on cohorts, the objectives are to describe the natural history of the studied RD(s), establish phenotype-genotype correlations, decipher their pathophysiology, assess their societal and medico-economic impact, and/or identify patients eligible for new therapeutic approaches. Inclusion of prevalent and incident cases started at the end of 2016. As of April 2021, 5558 patients have been included within 13 RD e-cohorts covering 67 diseases integrated in 10 European Reference Networks and contributing to the European Joint Program on RDs. Several original results have been obtained in relation with the secondary objectives of the RaDiCo cohorts. They deal with discovery of new disease genes, assessment of treatment management, deciphering the underlying pathophysiological mechanisms, diagnostic approaches, genotype-phenotype relationships, development and validation of questionnaires relative to disease burden, or methodological aspects.Conclusion: RaDiCo currently hosts 13 RD e-cohorts on a sharable and interoperable platform constructed on the “cloud computing” principle. New RD e-cohorts at the European and international levels are targeted.
Source-tracking of the Chinese Chikungunya Viruses Suggests That Indian Subcontinent and Southeast Asia Act as Major Hubs for the Recent Global Spread of Chikungunya Virus
Authors: Qiu Shaofu , Guo Jinpeng , Li Peihan , Li Peng , Du Xinying , Hao Rongzhang , Yang Chaojie , Wang Qi , Liu Hongbo , Zhang Haoran , Tian Sai , Shi Hua , Wen Liang , An Daizhi , Yang Xiaocui , An Xiaoyuan , Wang Ligui , Song Hongbin , Wang Changjun 
Published: June 2021

Abstract
BackgroundChikungunya fever caused by Chikungunya virus (CHIKV) has become a major global health concern, which has caused un­expected large outbreaks in Africa, Asia, Europe, and the Ameri­cas. China is a non-indigenous region of CHIKV, and the origin of the Chinese CHIKV strains is not well understood. Especially, there is limited understanding of the recent global spread of CHIKV in the context of recent worldwide epidemic of CHIKV.MethodsHere we investigated a novel Chikungunya patient imported from Myanmar into China in August, 2019. The genome was sequenced directly from the serum sample by combined MinION sequencing with BGISEQ-500 sequencing. We constructed a complete CHIKV genome dataset, including 727 CHIKV genomes retrieved from Genbank and the genome sequenced in this study, and conducted an updated and comprehensive phylogenetic analysis to understand the origin, evolution, transmission routes and genetic adaptation of the virus.ResultsAll of the globally distributed CHIKV genomes were divided into three genotypes, including West Africa (WA), East/Central/South African (ECSA) and Asian genotypes. The genome sequenced in this study was located in the Indian Ocean lineage (IOL), and closely related to a strain isolated from a patient in Australia who returned from Bangladesh in 2017. Comprehensive phylogenetic analysis showed that the Chinese strains were mainly sourced from Indian subcontinent and Southeast Asia. Further analyses indicated that Indian subcontinent and Southeast Asia may act as major hubs for the recent global spread of CHIKV, leading to widespread epidemics and even many large outbreaks. Moreover, we identified 179 distinct sites containing some undescribed sites in the sP and nsP proteins, showing obvious genetic varieties associated with different CHIKV lineages.ConclusionsHere we reported a novel CHIKV isolate from a Chikungunya patient imported from Myanmar into China in 2019, and summarized the source and evolution of Chinese CHIKV strains. The present findings provided better understanding of the recent global evolution of CHIKV, highlighting the urgent need for the strengthened surveillance including the viral diversity surveillance.
Melatonin Attenuates Ropivacaine-Induced Apoptosis by Inhibiting Excessive Mitophagy Through the Parkin / PINK1 Pathway in PC12 and HT22 Cells
Authors: Zeng Lian , He Jiafeng , Liu Chenguang , Zhang Fuyu , Zhang Zhen , Chen Huaxian , Wang Qingsong , Ding Xudong , Luo Huiyu 
Published: June 2021

Abstract
Melatonin, as an endogenous circadian indoleamine, is secreted from the pineal gland and has extensive biological functions, including antioxidant, anti-inflammatory, anti-tumor and neuroprotective effects. Its neuroprotective effects make it to be a potential therapeutic for many nerve damages, but its effect on ropivacaine-induced neurotoxicity remains unclear. The aims of our research were to explore the impact and mechanism of melatonin on ropivacaine-induced neurotoxicity. Our results shown that melatonin pretreatment protected the cell viability, morphology, and apoptosis of PC12 and HT22 cells from ropivacaine, it also improved ropivacaine-induced mitochondrial dysfunction and it inhibited an increase of mitophagy levels by ropivacaine. In addition, our study also revealed that the increase of mitophagy levels could reduce the protective effect of melatonin on ropivacaine-induced apoptosis in PC12 and HT22 cells, but the inhibition of mitophagy could enhance this effect of melatonin. To further illustrate the mechanism of melatonin inhibited mitophagy, the expressions of Parkin and PINK1 were detected, and results shown that melatonin inhibited the activation of Parkin / PINK1 pathway. In conclusion, our results indicated that melatonin protected ropivacaine-induced apoptosis through suppressing excessive mitophagy by inhibiting the Parkin / PINK1 pathway. Melatonin may be a useful potential therapeutic agent against ropivacaine-induced neurotoxicity.
High Prevalence of Antinuclear Antibodies in Patients With Chronic Hepatitis C Virus Infection
Authors: de Castro Geison Luiz Costa , Amoras Ednelza Silva Graça , Araujo Mauro Sérgio , Conde Simone Regina da Silva Souza , Bichara Carlos David Araújo , Queiroz Maria Alice Freitas , Vallinoto Antonio Carlos Rosário 
Published: June 2021

Abstract
Hepatitis C virus (HCV) infection is a serious public health concern due to its high prevalence and mortality rate. In chronic infection, HCV may induce autoimmune responses through the production of autoantibodies, including anti-nuclear antibodies (ANA). We assessed the presence of ANA by indirect immunofluorescence using HEp-2 cells in 89 patients with chronic hepatitis C. We also collected data on epidemiological variables; clinical characteristics; and biochemical, hematological, molecular and histopathological information from the patients to assess the impact of the presence of ANA in those patients. The prevalence of ANA in the patients was 20.2%, which was significantly higher than that found in healthy controls (2%). However, there was no association of this marker with epidemiological, clinical-laboratory, molecular or histopathological characteristics of hepatitis C, although a slightly higher prevalence of ANA was detected in women and in patients infected with subgenotype 1a. In a specific analysis, chronic HCV patients with the “rods and rings” cytoplasmic pattern had higher degrees of hepatic fibrosis than did ANA-negative patients. The results suggest a greater predisposition to the presence of ANA in patients with HCV, which may be associated with a worse prognosis, especially in the presence of the “rods and rings” cytoplasmic pattern.
CO2 Dynamics and Heterogeneity in a Cave Atmosphere: Role of Ventilation Patterns and Airflow Pathways
Authors: Kukuljan Lovel , Gabrovsek Franci , Covington Matthew D. , Johnston Vanessa E. 
Published: June 2021

Abstract
Understanding the dynamics and distribution of CO2 in the subsurface atmosphere of carbonate karst massifs provides important insights into dissolution and precipitation processes, the role of karst systems in the global carbon cycle, and the use of speleothems for paleoclimate reconstructions. We discuss long-term microclimatic observations in a passage of Postojna Cave, Slovenia, focusing on high spatial and temporal variations of pCO2. We show 1) that the airflow through the massif is determined by the combined action of the chimney effect and external winds and 2) that the relationship between the direction of the airflow, the geometry of the airflow pathways, and the position of the observation point explains the observed variations of pCO2. Namely, in the terminal chamber of the passage, the pCO2 is low and uniform during updraft, when outside air flows to the site through a system of large open galleries. When the airflow reverses direction to downdraft, the chamber is fed by inlets with diverse flow rate and pCO2, which enter via small conduits and fractures embedded in a CO2-rich vadose zone. If the spatial distribution of inlets and outlets produces minimal mixing between low and high pCO2 inflows, high and persistent gradients in pCO2 are formed. Such is the case in the chamber, where vertical gradients of up to 1000 ppm/m is observed during downdraft. The results presented in this work provide new insights into the dynamics and composition of the subsurface atmosphere and demonstrate the importance of long-term and spatially distributed observations.
Tibial Cortex Transverse Distraction Combined With Debridement and Vacuum Sealing Drainage for Diabetic Foot Ulcers: Validation of Previous Published Study
Authors: Fan Zhi-Qiang , Liu De-Wu 
Published: June 2021

Abstract
Objective: Tibial cortex transverse distraction (TCTD) has been recently reported in treating diabetic foot ulcers. However, there is no further studies verifying the effectiveness. We performed TCTD combined with debridement and vacuum sealing drainage (VSD) for diabetic foot ulcers to test the effectiveness.Methods: This study includes 25 patients with diabetic foot ulcers from 3 hospitals. The ulcers of all the cases didn't heal for over 3 months. Then we performed TCTD combined with debridement and VSD for them. After the surgery, the patients were regularly followed-up for more than one year.Results: Among all the patients, one case underwent amputation because of aggravated infections. There were no cases with complication like tibia fractures. Pin-site infections occurred in 2 patients. The infected pin-site healed after changing dressings for 2 weeks. For the rest 22 patients, their ulcers healed at postoperative 8.2 ± 4.5 weeks. The postoperative visual analogue score (VAS) which indicated pain degree, reduced significantly when compared with preoperative VAS.Conclusions: When combined with other measures like debridement and VSD, TCTD can bring advantages for patients diagnosed with diabetic foot ulcers, and the trauma of this operation remains a factor to be considered.Type of study/level of evidence: Therapeutic IV.
Cancer-Associated Adipocytes Release FUCA2 to Promote Aggressive Phenotype in Triple-Negative Breast Cancer
Authors: Liu Qun , Dong Hui-ting , Zhao Tingting , Yao Fan , Xu Yingying , Chen Bo , Wu Yunfei , Jin Feng , Xing Peng 
Published: June 2021

Abstract
Background: Cancer-associated adipocytes (CAAs) have been suggested to promote tumor progression. Yet, the role of CAAs in triple-negative breast cancer (TNBC) is poorly investigated. Methods: We compared the expression of secretory protein-encoding genes in CAAs and control adipocytes. The effect of key secretory protein(s) on TNBC cell behaviors was explored. Results: CAAs expressed and secreted FUCA2 at greater levels than control adipocytes. When FUCA2 activity was blocked with a neutralizing antibody, TNBC cell proliferation and migration induced by CAA conditioned medium was impaired. In contrast, supplement of exogenous FUCA2 protein reinforced the proliferation, colony formation, and migration of TNBC cells. In vivo studies confirmed that FUCA2 exposure enhanced tumorigenesis and metastasis of TNBC cells. Mechanistic investigation revealed that FUCA2 induced TNBC aggressiveness through TM9SF3-dependent signaling. Depletion of TM9SF3 blocked CAA- and FUCA2-induced TNBC cell proliferation and migration. Compared to adjacent breast tissues, TNBC tissues had increased expression of TM9SF3. Moreover, high TM9SF3 expression was associated with advanced TNM stage, lymph node metastasis, and shorter overall survival of TNBC patients. Conclusions: Altogether, CAAs secrete FUCA2 to promote TNBC growth and metastasis through interaction with TM9SF3. Inhibition of TM9SF3 may represent a potential therapeutic strategy in the treatment of TNBC.
Dopaminergic Receptors as Neuroimmune Mediators in Experimental Autoimmune Encephalomyelitis
Authors: Gonçalves Elaine Cristina Dalazen , Lieberknecht Vicente , Horewicz Verônica Vargas , Rabelo Bruna Daniel , Felipetti Francielly Andressa , Rodrigues Ana Lúcia Severo , Martins Daniel Fernandes , Dutra Rafael Cypriano 
Published: June 2021

Abstract
The dopaminergic system plays an essential role in maintaining homeostasis between the central nervous system (CNS) and the immune system. Previous studies have associated imbalances in the dopaminergic system with the pathogenesis of multiple sclerosis (MS). Herein, we investigate the protein levels of dopaminergic receptors (D1R and D2R) in different phases of the experimental autoimmune encephalomyelitis (EAE) model. We also examined if the treatment with pramipexole (PPX) – a dopamine D2/D3 receptor-preferring agonist – would be able to prevent the EAE-induced motor and mood dysfunction, as well as the underlying mechanisms of action. Our findings showed that D2R immunocontent upregulated in the spinal cord of EAE mice 14 days post-induction. Moreover, D1R and D2R immunocontent was significantly increased in the lymph nodes during the chronic phase of EAE. Also, during this phase, oxidative damage in the spinal cord and striatum of EAE animals was significantly higher. During the pre-symptomatic phase, axonal damage in the spinal cord of EAE mice could already be found. In addition, PPX treatment failed to inhibit EAE progression and anhedonic-like behavior. Otherwise, PPX inhibited the depressive-like behavior. Relevantly, PPX oral treatment downregulated IL-1β levels and increased BNDF content in the spinal cord after EAE induction. Altogether, it is possible to conclude that D2R participates in crosstalk between CNS and immune system during autoimmune and neuroinflammatory response induced by EAE, mainly in the acute and chronic phase of the disease. Relevantly, a dopamine D2/D3 receptor-preferring agonist mitigated depressive-like behavior induced by EAE, which offers a new possibility for treating depressive symptoms in MS patients.
Using Ant Colony Algorithm on Scheduling Strategy Based on Docker Cloud Platform
Authors: Wu Guanqaun , Chen Rongli , Zen Desheng , Chen Xiaozhong 
Published: June 2021

Abstract
With the development of Internet technology, the combination of industrial intelligence and data mining technology has played a huge role in the development of various industries, and has also become an inevitable demand for the development of information society. The cloud service can solve the problem of data processing conveniently and quickly, so it becomes particularly important for the development of cloud service. Traditional cloud service applications cannot meet users' urgent needs for services in terms of rapid release and efficient allocation of resources. Container technology has emerged to meet this challenge. Containers as a better resource virtualization technology will naturally be widely recognized and favored by the industry. Container cloud platform has the advantages of small redundant environment, high resource utilization, fast deployment, flexible and scalable. The proposed method use task scheduling of container cloud platform is to allocate available resources to users according to their different service demands. The result of this paper can ensure the effective execution of user container tasks.
Aristolochic Acid Nephropathy as a Potential Model of Renal Senescence
Authors: Urate Shingo , Wakui Hiromichi , Azushima Kengo , Yamaji Takahiro , Suzuki Toru , Abe Eriko , Tanaka Shohei , Taguchi Shinya , Tsukamoto Shunichiro , Kinguchi Sho , Uneda Kazushi , Kanaoka Tomohiko , Atobe Yoshitoshi , Funakoshi Kengo , Yamashita Akio , Tamura Kouichi 
Published: June 2021

Abstract
The kidney is among the organs most susceptible to age-associated impairments. Although there has recently been extensive research on renal aging, appropriate models remain limited. Generally, renal aging is strongly associated with renal fibrosis, which is the final common pathway of chronic kidney disease. Aristolochic acid (AA), a nephrotoxic agent, causes aristolochic acid nephropathy (AAN), characterized by progressive renal fibrosis and functional decline. Here, we examined the potential of AAN as a model of renal senescence by chronically administering AA to C57BL/6 mice. Compared with controls, the AA group presented aged kidney-like phenotypes such as renal atrophy, renal functional decline, and tubulointerstitial fibrosis. Additionally, AA promoted cellular senescence specifically in the kidney, concomitant with increase in renal p16 mRNA expression and senescence-associated β-galactosidase activity. Furthermore, AA-treated mice exhibited proximal tubular mitochondrial abnormalities, followed by accumulation of reactive oxygen species. Additionally, Klotho, an antiaging gene, was significantly decreased in the kidney of AA-treated mice. Collectively, the results of the present study indicate that AAN partially mimics the aged kidney and may serve as a useful mouse model for research on renal aging.
Antibacterial, Antibiofilm and Anti-adhesion Activities of Piper Betle Leaf Extract Against Avian Pathogenic Escherichia Coli
Authors: Kulnanan Pawinee , Chuprom Julalak , Thomrongsuwannakij Thotsapol , Romyasamit Chonticha , Sangkanu Suthinee , Manin Nannaphat , Nissapatorn Veeranoot , Pereira Maria de Lourdes , Wilairatana Polrat , Mitsuwan Watcharapong 
Published: June 2021

Abstract
Piper betle leaves have traditionally been used to treat many diseases, including bacterial infections. The present study aimed to investigate the antibacterial, antibiofilm, and anti-adhesion activities of P. betle extract against Avian pathogenic Escherichia coli (APEC). The ethanol extract of P. betle leaves demonstrated strong antibacterial activity against clinical isolates of APEC with MIC and MBC values ranging from 0.5-1.0 mg/mL. Disruption and breakdown of the bacterial cells were detected when the cells were challenged with the extract at 2×MIC. Bacterial cells treated with the extract demonstrated longer cells without a septum, compared to the control. The extract at 1/8, 1/4, and 1/2×MIC significantly inhibited the formation of bacterial biofilm of the isolates (P<0.05) without inhibiting growth. At 1/2×MIC, 55% of the biofilm inhibition was detected in APEC CH09, a strong biofilm producer. At 32×MIC, 88% of the inhibition of viable cells embedded in the mature biofilm was detected in APEC CH09. Reduction in the bacterial adhesion to surfaces was shown when APEC were treated with sub-MICs of the extract as observed by SEM. The results suggested potential medicinal benefits of P. betle extract for the treatment of the infection caused by Avian pathogenic E. coli.
Gastrosplenic Fistula Due to Splenic Lymphoma: Two Case Reports and Literature Review
Authors: Letaief Feryel Ksontini , Nefzi Issaad , khrouf salim , ouali Sonia , Zidi Asma , Magherbi houcine , Ayadi Mouna 
Published: June 2021

Abstract
PurposeGastrosplenic fistula (GSF) is a rare and potentially fatal complication of various diseases, of which lymphoma is the most common cause. We aim through our work to relate two cases of GSF and to review literature. Methods We reviewed two cases treated in our department of GSF and made a research in Pubmed using the keywords “Gastrosplenic fistula” and “Splenic lymphoma”.Results GSF is a rare condition that can occur spontaneously or after initiation of chemotherapy. It arises from the rapid growth of tumour and invasion of surrounding organs. Diagnosis may be difficult to make and confused with splenic abscess. Treatment modalities include surgical resection, chemotherapy or a combination of both. Here we report two cases of GSF due to diffuse large B cell lymphoma patients. The first case is of a 54-year-old woman with a spontaneous fistula in the stomach. The second one is of a 48 year-old- male patient presenting a fistula after chemotherapy. Both patients died after surgery. ConclusionGSF is a rare but dangerous condition in which surgery is currently the preferred treatment.