bluuebunny/crossref_metadata_2025_split huggingface dataset:
The effect of GSK-3β in arsenic-induced apoptosis of malignant tumor cells: a systematic review and meta-analysis
Authors: Gao Xin , Deng Bin , Ran Shanshan , Li Shugang 
Published: February 2021

Abstract
Purpose: Arsenic has been reported to induce apoptosis in malignant tumor cells, therefore, it may be regarded as a treatment for some cancers. The mitochondrial apoptosis pathway, mediated by GSK-3β, plays an important role in tumor cell apoptosis. Nonetheless, the regulation of GSK-3β by arsenic remains controversial. Materials and Methods: We included 19 articles, which conducts the role of GSK-3β in the process of arsenic-induced tumor cell apoptosis by the meta-analysis. Results: Compared with the control group, the expression of GSK-3β (SMD=-0.92,95% CI (-1.78,-0.06)), p-Akt (SMD=-5.46,95% CI (-8.67,-2.24)) were reduced in the arsenic intervention group. Meanwhile, the combined treatment of arsenic and Akt agonist can inhibit the expression of p-GSK-3β. Using the dose and time subgroup analysis, it was shown that the low-dose and sub-chronic arsenic exposure could inhibit the expression of p-Akt (P<0.05). In the subgroup analysis of GSK-3β sites, arsenic could inhibit p-Akt and GSK-3β (Ser9) (SMD =-0.95, 95% CI (-1.56,-0.33)). There was a dose-related effect seen between arsenic (≤8 μmol/L) and p-GSK-3β, and the expression of p-GSK-3β was gradually followed by the arsenic dose. When arsenic acted on GSK-3β (ser9), the expression of Mcl-1 and pro-caspase-3 were dropped, while the loss rate of mitochondrial membrane potential and cleaved-caspase-3 were increased significantly (P<0.05). Conclusion: This study revealed that arsenic could inhibit the expression of GSK-3β (Ser9) and then induce tumor cell apoptosis. It might be correlated with arsenic inhibiting p-Akt, down-regulating GSK-3β, and triggering the Mcl-1-mediated mitochondrial apoptosis pathway.
Efficient lignin decomposing microbial consortium to hasten rice-straw composting with moderate GHGs fluxes
Authors: Dash Pradeep Kumar , Padhy Soumya Ranjan , Bhattacharyya Pratap , Pattanayak Ankita , Routray Soumya , Panneerselvam Periyasamy , Nayak Amaresh Kumar , Pathak Himanshu 
Published: February 2021

Abstract
We hypothised that lignin decomposition microbial consortium would make rice-straw decomposition faster as straw contain around 15–24% lignin. In this study, we isolated lignin degrading microbes from four natural sources and based on their ability towards lignin degradation four microbial strains and their combination (2 bacteria (LB 8, LB 18) and 2 fungi (LF 3, LF 9) were selected for rice straw decomposition. During straw decomposition greenhouse gases emission, enzymatic activities (β-glucosidase, cellulase, laccase), reduction in lignin content, weight loss and carbon nitrogen ratio (C:N) were quantified. The β-glucosidase, cellulase and laccase activities were higher in LB 18 + LF 3 consortium as compared to others. The lignin content was also decreased (8.9–9.5 to 6.6–7.9%) continuously from initial to 28th days of composting under LB 18 + LF 3. We found the microbial consortium LB 18 + LF 3 decomposed the rice straw faster as indicated by reduction of C:N ratio and reduction of lignin, hemicellulose and cellulose contents of 60, 19.2, 41.5 and 10.3%, respectively at 28th day from initial compare to other strains/consortium. However, higher, CH4 and CO2 fluxes were observed at 28th days after composting (1.36 and 200.7 mg m− 2 h− 1) with no significant trend in N2O flux. Further, the consortium identified could be tested for in-situ straw decomposition with proper moisture management to evaluate its potential in field condition. Therefore, we conclude that use of lignin decomposing microbial consortium has the potential to hasten the composting of rice straw in large scale, so viable option to reduce the menace of straw burning.
Preparation of Cellulose-based fluorescent materials as coating pigment by use of reversible DMSO/DBU/CO2 system
Authors: Cao Qinghua , Dai Jinyue , Bao Xin , Zhang Zhenyu , Liu Fei , Feng Yuhong , Na Haining , Zhu Jin 
Published: February 2021

Abstract
A series of cellulose-based fluorescent materials are prepared under relative mild condition by use of the particular reversible DMSO/DBU/CO2 system to utilize as coating pigments. Through the observation under 365nm UV light, the cellulose-based fluorescent materials exhibit good fluorescence response and bright color. Furthermore, due to the limitation of the molecular skeleton of cellulose, the intrinsic aggregation caused quenching phenomenon commonly existed in conventional organic fluorescent pigments can be effectively inhibited, which is very helpful to retain good fluorescence response in epoxy-based coating material and its coating films. Moreover, the addition of cellulose-based fluorescent materials also increases the mechanical properties of the coating film. The increase of tensile strength and tensile modulus respectively reaches ~ 39% and ~ 66%. Solvent resistance and thermal property of the coating films generally remain unchanged. The fabrication of cellulose-based fluorescent materials in DMSO/DBU/CO2 system provides a feasible way to develop the functional application of cellulose.
Evaluation of the effects of human dental pulp stem cells on the biological phenotype of hypertrophic keloid fibroblasts
Authors: Fu Ling-ling , Yan Ming , Nada Ola A. , Chen Li-ming , Gosau Martin , Smeets Ralf , feng hongchao , Friedrich Reinhard E. 
Published: February 2021

Abstract
Background Despite numerous existing treatments for keloids, the responses in the clinic have been disappointing due to either low efficacy or side effects. Numerous studies dealing with preclinical and clinical trials have been published about effective therapies for fibrotic diseases using mesenchymal stem cells, However, no research has yet been reported to scientifically investigate the effect of HDPSCs on the treatment of keloids.The objective is to provide an experimental basis to provide a novel proposal for the application of stem cells in the treatment of keloids.Methods HNFs and HKFs were cultured alone and in combination with DPSCs using a trans-well cell-contact-independent cell culture system. The effects of DPSCs on HKFs were tested using CCK-8 assay, Live/dead staining assay, quantitative polymerase chain reaction, Western blot．Results DPSCs don’t inhibited HKFs and HNFs proliferation and induce apoptosis, but inhibited migration. Furthermore, DPSCs significantly decreased expression of profibrotic genes, transforming growth factor-β 1，and transforming growth factor-β 2. DPSCs suppressed the ECM synthesis in HKFs, as indicated by decreased expression of collagen I and fibronectin and low levels of hydroxyproline in cell culture supernatant． Conclusions 1) Co-culture of DPSCs inhibits the migration of HKFs but does not affect the proliferation and apoptosis of HKFs.2) DPSCs co-culture inhibits the expression of pro-fibrotic genes and promotes the expression of anti-fibrotic genes in HKFs.3) DPSCs co-culture inhibits the synthesis of extracellular matrix by HKFs.
Laser Ablation of Titanium Alloy (Ti64): Effects of Process Parameters on Performance of Laser Welded Ti64 – Polyamide 6.6 Joints.
Authors: Al-Sayyad Adham , Salah Farah , Bardon Julien , Hirchenhahn Pierre , Shihata Lamia , Houssiau Laurent , Plapper Peter 
Published: February 2021

Abstract
Laser welding of metals – polymers has gained strong scientific and industrial interest because of its ability to produce miniaturized joints in lightweight products with customized properties. Surface pretreatments before joining process have shown significant impact on enhancing properties of laser welded metal – polymer joints. This work adopts a Design of Experiments (DoE) approach to investigate the influence of titanium alloy (Ti64) laser ablation parameters on the performance of laser welded Ti64 – polyamide (PA6.6) assemblies. In this first study, significant laser ablation parameters were highlighted, process window outlined, and optimal parameters identified. Laser ablation pretreatment parameters demonstrated a strong influence on joint resistance to failure. Effects of laser ablation parameters on titanium surface morphology were analyzed using Scanning Electron Microscope (SEM). In a second study, the effects of ablation parameters on Ti64 surface properties and welding quality will be investigated.
COVID-19 fake news diffusion across Latin America
Authors: Ceron Wilson , Sanseverino Gabriela Gruszynski , de-Lima-Santos Mathias-Felipe , Quiles Marcos G. 
Published: February 2021

Abstract
Fact-checking verifies a multitude of claims and remains a promising solution to fight fake news. The spread of rumors, hoaxes, and conspiracy theories online is evident in times of crisis, when fake news ramped up across platforms, increasing fear and confusion amongst the population as seen in the COVID-19 pandemic. This article explores fact-checking initiatives in Latin America, using an original Markov-based computational method to cluster topics on tweets and identify their diffusion between different datasets. Drawing on a mixture of quantitative and qualitative methods, including time-series analysis, network analysis and in-depth close reading, our article proposes an in-depth tracing of COVID-related false information across the region, comparing if there is a pattern of behavior through the countries. We rely on the open Twitter application programming interface (API) connection to gather data from public accounts of the six major fact-checking agencies in Latin America, namely: Argentina ( Chequeado ), Brazil ( Agência Lupa ), Chile ( Mala Espina Check ), Colombia ( Colombia Check from Consejo de Redacción ), Mexico ( El Sabueso from Animal Político ) and Venezuela ( Efecto Cocuyo ). In total, these profiles account for 102,379 tweets that were collected between January and July 2020. Our study offers insights into the dynamics of online information dissemination beyond the national level and demonstrates how politics intertwine with the health crisis in this period. Our method is capable of clustering topics in a period of overabundance of information, as we fight not only a pandemic but also an infodemic, evidentiating opportunities to understand and slow the spread of false information.
Intensive Care Unit and Hospital Outcomes of Patients Admitted with Blastomycosis: A 14-Year Retrospective Study
Authors: Almodallal Yahya , Alkurashi Adham K , Albitar Hasan Ahmad Hasan , Jenad Hussam , Prueksaritanond Suartcha , Tedja Rudy , Pannu Bibek , Jagtap Prashant , iyer vivek 
Published: February 2021

Abstract
Introduction: Blastomycosis is an uncommon; potentially life threatening granulomatous fungal infection. The aim of this study is to report hospital and intensive care unit (ICU) outcomes of patients admitted with blastomycosis. Methods: All patients admitted for treatment of blastomycosis at the Mayo Clinic-Rochester, Minnesota between 01/01/2006 and 09/30/2019 were included. Demographics, comorbidities, clinical presentation, ICU admission, and outcomes were reviewed.Results: A total of 84 Patients were identified with 93 unique hospitalizations primarily for blastomycosis. The median age at diagnosis was 49 (IQR 28.1-65, range: 6-85) years and 56 (66.7%) were male. The most frequent comorbidities incl­uded hypertension (n=28, 33.3%); immunosuppressed state (n=25, 29.8%) and diabetes mellitus (n=21, 25%). The lungs were the only organ involved in 56 (66.7%) cases and the infection was disseminated in 19 (22.6%) cases. A total of 29 patients (34.5%) underwent ICU admission due to complications of blastomycosis. ICU related events included mechanical ventilation (n=21, 25%), acute respiratory distress syndrome (ARDS) (n=13, 15.5%), tracheostomy (n=9, 10.7%), renal replacement therapy (n=8, 9.5%), and extracorporeal membrane oxygenation (ECMO) (n=4, 4.8%). A total of 12 patients (14.3%) died in the hospital; all of whom had undergone ICU admission. In-hospital mortality was associated with renal replacement therapy (RRT) (P=0.0255).Conclusions: Blastomycosis is a serious, potentially life-threatening infection that results in significant morbidity and mortality with a 34.5% ICU admission rate. Renal replacement therapy was associated with in-hospital mortality.
Highly-porous Uniformly-sized Amidoxime-functionalized Cellulose Beads Prepared by Microfluidics with N-methylmorpholine N-oxide
Authors: Baek Seung-Yeop , Park Soo-Young 
Published: February 2021

Abstract
Uniformly-sized porous cellulose beads functionalized with amidoxime groups were prepared for the first time using a microfluidic method with N-methylmorpholine N-oxide (NMMO) monohydrate as a cellulose solvent. The molten state cellulose dope in NMMO monohydrate (cell/NMMO dope) as a disperse phase and hot mineral oil as a continuous phase were used in a T-junction microfluidic chip to produce uniformly-sized cell/NMMO droplets. Coagulation of the molten state cell/NMMO droplet at high temperature and amidoxime functionalization could prepare the highly-porous spherical amidoxime-functionalized cellulose beads with a uniform fibrous open internal structure. The prepared amidoxime-functionalized cellulose beads showed excellent metal adsorption properties with a maximum adsorption capacity of ~ 80 mg g− 1 in the case of Cu2+/phthalate ions. The newly developed highly-porous cellulose beads can open many new applications with other proper functionalization at the reactive hydroxyl groups of the cellulose.
Exploration of Prognostic Biomarkers and Therapeutic Targets in The Microenvironment of Bladder Cancer Based on CXC Chemokines
Authors: Sun Xiaoqi , Chen Qunxi , Zhang Lihong , Chen Jiewei , Zhang Xinke 
Published: February 2021

Abstract
Background: Bladder cancer (BLCA) has a high rate of morbidity and mortality, and is considered as one of the most malignant tumors of the urinary system. Tumor cells interact with surrounding interstitial cells, playing a key role in carcinogenesis and progression, which is partly mediated by chemokines. CXC chemokines exert anti‑tumor biological roles in the tumor microenvironment and affect patient prognosis. Nevertheless, their expression and prognostic values patients with BLCA remain unclear. Methods: We used online tools, including Oncomine, UALCAN, GEPIA, GEO databases, cBioPortal, GeneMANIA, DAVID 6.8, Metascape, TRUST (version 2.0), LinkedOmics, TCGA, and TIMER2.0 to perform the relevant analysis.Results: The mRNA levels of C-X-C motif chemokine ligand (CXCL)1, CXCL5, CXCL6, CXCL7, CXCL9, CXCL10, CXCL11, CXCL13, CXCL16, and CXCL17 were increased significantly increased, and those of CXCL2, CXCL3, and CXCL12 were decreased significantly in BLCA tissues as assessed using the Oncomine, TCGA, and GEO databases. GEO showed that high levels of CXCL1, CXCL6, CXCL10, CXCL11, and CXCL13 mRNA expression are associated significantly with the poor overall survival (all p < 0.05), and similarly, those of CXCL2 and CXCL12 in the TCGA database (p < 0.05). The predominant signaling pathways involving the differentially expressed CXC chemokines are cell cycle, chemokine, and cytokine-cytokine receptor interaction. Moreover, transcription factors such as Sp1 transcription factor (SP1), nuclear factor kappa B subunit 1 (NFKB1), and RELA proto-oncogene, NF-KB subunit (RELA) were likely play critical roles in regulating CXC chemokine expression. LYN proto-oncogene, src family tyrosine kinase (LYN) and LCK proto-oncogene, src family tyrosine kinase (LCK) were identified as the key targets of these CXC chemokines. MicroRNAs miR200 and miR30 were identified as the main microRNAs that interact with several CXC chemokines through an miRNA-target network. The expression of these chemokines is closely associated with the inﬁltration of six categories of immune cells.Conclusions: We explored the CXC chemokines superfamily-based biomarkers associated with BLCA prognosis using public databases, and provided possible chemokine targets for patients with BLCA.
Diclofenac Sodium Determination in Pharmaceutical Preparations After Complexation With Calcium and Magnesium and Molecular Modelling Study.
Authors: Soomro Waheed Ali , Khuhawar Muhammad Yar , Jahaangir Taj Muhammad , Lanjwani Muhammad Farooque , Brohi Rafi-u-Zaman , Rind Imran Khan 
Published: February 2021

Abstract
The authors have requested that this preprint be removed from Research Square.
Vacancy-Defect Modulated Pathway of Photoreduction of CO2 on Quaternary Single Atomically Thin AgInP2S6 Sheets toward Boosting Efficient and Selective Production of Value-Added Olefiant Gas
Authors: Zhou Yong , Zou Zhigang , Gao Wa , Wang Xiaoyong , Shen Qing , He Huichao , Yujie Xiong , Wang Jinlan , Shi Li , Yang Yong , Cheng Zhenxiang , Li Xiaoning 
Published: February 2021

Abstract
The quaternary AgInP2S6 atomic layer with the thickness of ~ 0.70 nm were successfully synthesized through facile ultrasonic exfoliation of the corresponding bulk crystal. The ultrathin sheet exhibits efficiently photocatalytic conversion of CO2 into CO as a major product and minority of CH4 and C2H4 in the presence of water vapor. The sulfur defect engineering on this atomic layer through a H2O2 etch process can excitingly enable to change the CO2 photoreduction reaction pathway to steer dominant generation of ethene (C2H4) important chemical with the yield-based selectivity reaching ~73% and the electron-based selectivity as high as ~89%, and the quantum yield of 0.51% at wavelength of 415 nm. Both DFT calculation and in-situ FTIR demonstrate as the introduction of S vacancies in AgInP2S6 causes the charge accumulation on the Ag atoms near the S vacancies, the exposed Ag sites can thus effectively capture the forming *CO molecules, making the catalyst surface enrich with key reaction intermediates to lower the C-C binding coupling barrier, which facilitates the production of C2H4. Surface photovoltage measurement confirms that atomically ultrathin structure of the exfoliated AgInP2S6 can shorten the transfer distance of charge carriers from the interior onto the surface, thus decrease the recombination in body and improve the catalytic efficiency. This work may provide fresh insights into the design of atomically thin photocatalyst framework for CO2 reduction and establish an ideal platform for reaffirming the versatility of defect engineering in tuning catalytic activity and selectivity.
Morphology and Hemispheric and Gender difference of the Anterior Ascending Ramus and the Horizontal Ascending Ramus of the Lateral Sulcus
Authors: Wang Yu , liu shuwei , Xu Fei fei , Hou Lan wei , Zhou Wen Juan , Tang Yu chun 
Published: February 2021

Abstract
Broca’s area is composed of the pars opercularis (PO) and the pars triangularis (PTR) of the inferior frontal gyrus，which was separated by the anterior ascending ramus of lateral sulcus (aals) and the horizontal ascending ramus of the lateral sulcus (hals). The morphometry of these two sulci maybe have potential effects on the various functions of Broca’s area. To explore the morphology variation, hemispheric and gender difference of these two sulci, so as to enable better understanding of their patterns and the functions of the Broca’s area. BrainVISA was used to reconstruct and parameterize these two sulci with 3D MR images of 90 healthy right-handed subjects. The 3D anatomic morphology of these two sulci was presented using 4 sulcal parameters, including average depth (AD), average width (AW), top length (TL) and bottom length (BL). The aals could be identified in 98.89% of the hemispheres, while the hals could be identified in 98.33%. The morphological patterns of these two sulci were categorized into four main types. There were no statistically significant interhemispheric and gender differences in the frequency of the morphological patterns. There was statistically significant interhemispheric difference in BL of the aals and AW of the hals. Significant gender differences were found in the bilateral AD of the aals and the BL and TL of the hals in right hemisphere. Our results will not only provide structural basis for the functional studies that related to Broca’s area, but also be helpful for the precise positioning of special structures in neurosurgery.
An Extended Analysis on Tuning The Parameters of Adaptive Monte Carlo Localization ROS Package in an Automated Guided Vehicle
Authors: Reis Wallace Pereira Neves dos , Silva Guilherme José da , Junior Orides Morandin , Vivaldini Kelen Cristiane Teixeira 
Published: February 2021

Abstract
With a growth tendency, the employment of the Adaptive Monte Carlo Localization (AMCL) Robot Operational System (ROS) package does not reflect a more indepth discussion on its parameters tuning process. The authors usually do not describe it. This work aims to extend the analysis of the package’s parameters distinct influence in an Automated Guided Vehicle (AGV) indoor localization. The experiments test parameters of the filter, the laser model, and the odometry model. Extending the previous analysis of seven parameters, the present research discusses another ten from the 22 configurable parameters of the package. An external visual vehicle pose tracking is used to compare the pose estimation from the localization package. Although the article does not propose the best parameter tuning, its results discuss how each tested parameter affects the localization. The paper’s contribution is discussing the parameters variation impact on the AGV localization. It may help new researchers in the AMCL ROS package parameter tuning process. The results show minor changes in the default parameters can improve the localization results, even modifying one parameter at a time.
Composition of Urinary Stones in Children: Clinical and Metabolic Determinants in a French Tertiary Care Center
Authors: Rauturier Camille , Machon Christelle , Demède Delphine , Dubourg Laurence , Bacchetta Justine , Bertholet-Thomas Aurélia 
Published: February 2021

Abstract
The aim was to describe the composition of stones of pediatric patients, to illustrate current epidemiological trends in pediatric urolithiasis.Clinical and metabolic data from all pediatric patients with at least one stone that was analyzed by Fourier Transformed Infrared Spectroscopy (FTIR) in the Hospices Civils de Lyon s Civils de Lyon between 2013 and 2017 were retrospectively collected. A total of 111 patients (sex ratio 1.4:1) were included; their median [IQR] age was 7.5 [3.1-10.5] years. The main component of stones was calcium oxalate [weddellite for 34 (31%) stones, whewellite 23 (21%)], calcium phosphate [carbapatite 32 (29%), brushite 6 (5%), amorphous calcium phosphate 3 (3%)], struvite 5 (5%), cystine 4 (4%), uric acid 2 (2%), and ammonium acid urate 2 (2%). A total of 20 (18%) stones were pure and 24 (22%) were infectious. Carbapatite stones were the most frequent in patients < 2 years and calcium oxalate stones in patients > 2 years old. Metabolic abnormalities (most frequently hypercalciuria) were found in 50% of tested patients and in 54% of patients with infectious stones. Congenital anomalies of the kidney and/or urinary tract (CAKUT) or neurogenic bladder was present in 9/24 (38%) patients with infectious stones and 12/16 (76%) patients with bladder stones. Conclusion: This study confirms that calcium oxalate stones are the most frequent among pediatric patients, which could reflect the nutritional habits of predisposed patients. In contrast, infectious stones are less frequent and occur mostly in association with anatomic or metabolic favoring factors.