bluuebunny/crossref_metadata_2025_split huggingface dataset:
Shallow Temperature Lapse Rate Signified Elevation Dependent Warming in Different Treeline Environments in the Himalaya- Possible Implications to Treeline Vegetation
Authors: Joshi Rajesh , Tamang Ninchhen Dolma , Singh Surendra Pratap 
Published: November 2021

Abstract
There are emergent evidences that the rise in temperature in high altitude regions in comparison to low altitude of the Himalaya is more rapid than other parts of the World. This Elevation-dependent warming (EDW) can accelerate the rate of change in mountain ecosystems, including cryosphere, hydrology, biodiversity and socio-economic systems. In this paper, we present Temperature Lapse Rates (TLRs) from 20 stations for three treeline transects representing different climate regimes along the Himalayan arc. TLRs were calculated based on high temporal resolution data collected for two year (2017-18) from complex mountain terrain of treeline environment. The annual mean TLR increased with decreasing moisture, being markedly high at dry WH transect (-0.66℃/100 m) and lowest (-0.50℃/100 m) for moist EH transect. The One-Way ANOVA confirms that the TLR varied spatially, declining from West to East across the Himalayan arc, and significantly differ among seasons (F=3.2175; P = 0.03). The lowest mean TLRs were found during the winter season (EH: -0.46℃/100m; CH: -0.40℃/100m; WH: -0.31℃/100m). The monthly TLR varied within a narrow range (-0.49℃/100m to -0.54℃/100m) at EH transect, -0.24℃/100m to -0.68℃/100m at CH transect and from -0.26℃ to -0.90℃ at WH transect with lowest monthly TLR in December (-0.24 to -0.32℃/ 100m) for all three sites. Study shows moisture, snow albedo and reflectance play a key role as controlling factors on TLR in treeline environments. Higher growing season temperatures observed for treelines in Himalaya (8.4±1.8℃, 10.3±1.4℃, and 7.5±2.7℃) shows warmer treeline in Himalaya. The EDW may impact the dynamics of treeline, snow and moisture regime, surface energy balance, increased water stress, species distribution, and growing season of alpine vegetation in the Himalaya. The findings of the study could provide useful insight (ground-based) to re-parameterize the climate models over the Himalayan region. This study can facilitate improving interpolation of air temperature for ecological modeling studies in ungauged and the data-sparse regions, especially for the higher Himalaya where ground based station data are extremely scarce.
Extrapolating potential crop damage by insect pests based on land use data: examining inter-regional generality in agricultural landscapes
Authors: Tabuchi Ken , Takahashi Akihiko , Uesugi Ryuji , Okudera Shigeru , Yoshimura Hideto 
Published: November 2021

Abstract
ContextInter-regional relationships between landscape factors and biological responses under natural conditions are important but difficult to predict because of the differences in each landscape context and local environment. ObjectivesTo examine the inter-regional variability in relation to landscape factors and the biological response of an insect pest, we extrapolated a damage prediction model (‘the original model’) for rice using land-use data. We hypothesized that the original model would be applicable to new regions, but the predictive accuracy would be reduced. We predicted that adjusting the parameter coefficients would improve model performance (‘the adjusted model’). MethodsA field experiment was conducted in two regions that had a similar landscape context with the original region, in different years for each region, for four years in total. The proportion of rice damage and surrounding land-use within a 300-m radius was investigated. ResultsWhen ‘the original model’ was assigned to combined data from the original and extrapolated regions, the relationship between observed and predicted values was statistically significant, suggesting that there was an inter-regional common relationship. The relationship was not statistically significant if the model was applied only to the new regions. The adjusted model improved by 14% compared with the original model.ConclusionsThese results imply that in this pest–crop system, a common inter-regional biological response to arthropods is likely because of landscape factors, although local environmental factors must be considered. Application of such relationships is needed to identify or prevent pest hazards by offering region-wide management options.
Validation of Robotic-assisted Ureteroplasty with Buccal Mucosa Graft for Stricture at the Proximal and Middle Ureters: The First Comparative Study
Authors: Yang Che Hsueh , Lin Yi Sheng , Weng Wei Chun , Lu Chin Heng , Hsu Chao Yu , Tung Min Che , Ou Yen Chuan 
Published: November 2021

Abstract
Although ureteroplasty with buccal mucosa graft for long-segmental ureteral stenosis has been developed long ago, evidence was still restricted to case series in published literature. This study aims to validate ureteroplasty with buccal mucosa graft (BMG) in long-segment stricture at the proximal and middle ureters under designed comparative methods. From April 2015 to January 2019, we performed robotic-assisted ureteroplasty with BMG with a two-phase design and compared ureteroplasty and BMG (phase 2 surgery) with endoscopic stenting (phase 1 surgery). Paired data of effective renal plasma flow (ERPF), glomerular filtration rate (GFR), hydronephrosis grade, and physical and psychological domains of the World Health Organization Quality of Life (WHOQOL)-BREF were compared. A total of 29 patients were enrolled, and only three (10%) patients had hydronephrosis resolution after treatment with endoscopic stenting (p = 0.250 to baseline). Compared to endoscopic ureteral stent, Hedges’ g of ureteroplasty with BMG was 0.56 (95% CI: 0.43-0.69), 0.63 (95% CI: 0.46-0.80), 0.80 (95% CI: 0.56-1.04), and 1.06 (95% CI: 0.69-1.43) in EGFR, GFR, physical domain of WHOQOL-BREF, and psychological domain of WHOQOL-BREF, respectively (All significance; p<0.001). After 12-month follow-ups, no recurrence of stricture was reported. In conclusion, Robotic-assisted ureteroplasty with BMG onlay is efficient in reconstruction of long-segment stricture of the proximal and middle ureters.
Application of Synthetic Data to Establish the Working Framework for Multivariate Statistical Analysis of River Pollution Traceability - The Heavy Metals in Nankan River, Taiwan
Authors: Lin Chun-Chun , Lo Shang-Lien , Liou Sofia Ya-Hsuan 
Published: November 2021

Abstract
This study applied multivariate statistical analysis (MSA) to the synthetic data simulated by the river water quality model to investigate how two pollution sources with different characteristics and contributions affect the results of MSA. The results showed that when assessing the number and possible locations of pollution sources based on the results of cluster analysis (CA), hydrological information about surface water should be obtained to improve the accuracy of the results; when applying principal component analysis (PCA), the results of the second principal component (PC2) and the Pearson correlation coefficients among the pollutants should both be included, which can add more information about the characteristics of pollutant sources. In addition, this study found that the solid and liquid partition coefficients (Kd) of pollutants can affect the interpretation of the PCA results, so the Kd values should be determined before tracing the pollution sources to facilitate the evaluation of the source characteristics and potential targets. This study established a working framework for surface water pollution traceability to enhance the effectiveness of pollution traceability.
Intuitionistic Fuzzy Rough TOPSIS Method for Robot Selection using Einstein operators
Authors: Qadir Abbas , Naeem Muhammad , Abdullah Saleem , Ghanmi Nejib 
Published: November 2021

Abstract
Rough set and intuitionistic fuzzy set are very vital role in the decision making method for handling the uncertain and imprecise data of decision makers. The technique for order preference by similarity to ideal solution (TOPSIS) is very attractive method for solving the ranking and multi-criteria decision making (MCDM) problem. The primary goal of this paper is to introduce the Extended TOPSIS for industrial robot selection under intuitionistic fuzzy rough (IFR) information, where the weights of both, decision makers (DMs) and criteria are not-known. First, we develop Intuitionistic fuzzy rough (IFR) aggregation operators based on Einstein T-norm and T-conom, For this firstly we give the idea of intuitionistic fuzzy rough Einstein weighted averaging (IFREWA), intuitionistic fuzzy rough Einstein hybrid averaging (IFREHA) and intuitionistic fuzzy rough ordered weighted averaging (IFREOWA) aggregation operators. The fundamental properties of the proposed operators are described in detail. Furthermore to determine the unknown weights, a generalized distance measure are defined for IFRSs based on intuitionistic fuzzy rough entropy measure. Following that, the intuitionistic fuzzy rough information-based decision-making technique for multi-criteria group decision making (MCGDM) is developed, with all computing steps depicted in simplest form. For considering the conflicting attributes, our proposed model is more accurate and effective. Finally, an example of efficient industrial robot selection is presented to illustrate the feasibility of the proposed intuitionistic fuzzy rough decision support approaches, as well as a discussion of comparative outcomes, demonstrating that the results are feasible and reliable.
Negative Emotional Status and Influencing Factors of Young Employees in Center of Disease Control and Prevention
Authors: Han Lu , Li Qiyu , Zhang Yu , Liu Tuo , Ran Niu , Wang Qi , Zhao Lina 
Published: November 2021

Abstract
Objective To investigate the status and influencing factors of negative emotions among young employees in center of disease control and prevention. Methods Using the cluster sampling methods, 6099 employees aged 40 or below in center of disease control and prevention(CDC) of 32 province of China were interviewed by online questionnaire survey. The emotional conditions of anxiety and depression, and their influencing factors were analyzed. Results A total of 5,353 valid questionnaires were collected with the recovery rate of 87.77%. 2,871 cases of young employees had different degrees of negative emotions at work, accounting for about 53.60%. Regression analysis showed that gender, professional title, educational level, job satisfaction, chronic diseases, daily sleep duration, average weekly overtime, physical activity time, and sugary beverage intake were the influencing factors of negative emotions(P<0.05). Male, primary and below, never working overtime and daily physical activity time more than 30 minutes were protective factors for negative emotions(OR vale were 0.79, 0.68, 0.39 and 0.63, respectively, P<0.05). Bachelor degree or above, poor job satisfaction, chronic disease, daily sleep duration less than eight hours and drinking one to three sugary drinks a week were the risk factors for negative emotion(OR vale were1.21, 4.32, 2.16, 2.75 and 1.20, respectively, P<0.05). Conclusion Due to the influence of work pressure, lifestyle, chronic diseases and other factors, young employees in CDC have a certain degree of negative emotions at work, which should be paid enough attention. Meanwhile, corresponding measures should be taken according to the influencing factors to reduce the occurrence of negative emotions.
Effect of miR-192-5p on Osteogenic Differentiation of Bone Marrow Mesenchymal Stem Cells in Idiopathic Scoliosis by Regulating Wnt/β-catenin Signaling Pathway via RSPO1
Authors: Yang Yifan , Xu Jing , Su Qingxin , Wu Yiran , Li Qizheng , Ma zongren , ding tao 
Published: November 2021

Abstract
BackgroundIdiopathic scoliosis (IS) is the most common structural scoliosis, which seriously affects not only patient’s physical and mental health but also quality of patient’s life. Abnormal osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) is one of the causes of IS. However, the regulation mechanism of osteogenic differentiation of BMSCs in patients with IS remains to be further studied.MethodsSerum samples of 135 patients with IS were collected, and the expression of miRNA were detected by RT-qPCR. BMSCs from patients with IS were collected and the expression of miR-192-5p in BMSCs from IS patients and normal BMSCs was detected by RT-qPCR. Double luciferase reporter genes assay was used to verify the targeting relationship between miR-192-5p and RSPO1. The levels of RSPO1, osteogenic related proteins (OC, OPN and RUNX2) and Wnt/β-catenin signaling pathway related proteins (WNT3A and β-catenin) were detected by Western blotting. Alkaline phosphatase staining and alizarin red staining were used to evaluate the osteogenesis of BMSCs.ResultsmiR-192-5p was significantly up-regulated in serum and BMSCs of patients with IS. Alkaline phosphatase staining and alizarin red staining showed that miR-192-5p inhibitor promoted the osteogenic differentiation of BMSCs from IS patients. miR-192-5p targeted down-regulated the expression of RSPO1 in BMSCs from IS patients. In addition, overexpression of RSPO1 activated Wnt/β-catenin signaling pathway in BMSCs from IS patients. Furthermore, miR-192-5p/RSPO1 axis regulated levels of osteogenic related proteins (OC, OPN and RUNX2) in BMSCs from IS patients through Wnt/β-catenin signaling pathway, and affected the osteogenic differentiation of BMSCs.Conclusion﻿miR-192-5p, which was highly expressed in patients with IS, inhibited Wnt/β-catenin signaling pathway by down-regulating RSPO1 protein and then reduced the osteogenic differentiation ability of BMSCs.
Gender Differences in Prognosis after Primary Resection for Retroperitoneal Liposarcoma
Authors: Weng Cheng-Xin , Qi Yu-Han , Zhao Ji-Chun , Yuan Ding , Yang Yi , Wang Tie-Hao , Wang Jia-Rong , Huang Bin 
Published: November 2021

Abstract
Background: Current evidence regarding gender difference in retroperitoneal liposarcoma (RLPS) is scarce, we sought to investigate whether gender may affect prognosis after primary resection of RLPS.Methods: We used the Surveillance, Epidemiology, and End Results (SEER) database to identify RLPS patients from January 1973 to December 2015. Multivariate cox proportional hazards analysis was adopted to generate adjusted hazard ratio (AHR) and 95% confidence intervals (CI) of survival outcomes.Results: In total, 2108 RLPS patients, including 971 women and 1137 men, were identified, with a median follow-up of 45.0 (17.0-92.0) months. The 5-year and 10-year overall survival rates were 50.5% and 31.5% for men, and 60.4% and 42.5% for women. The 5-year and 10-year disease-specific survival rates for men and women were 71.5%, 57.3% and 76.3%, 62.1%, respectively. We found men were associated with an increased risk of all-cause mortality (AHR 1.3, 95%CI 1.0-1.6, P=.017) but not disease-specific mortality (AHR 1.2, 95%CI 0.9-1.6, P=.246). The subgroup analyses revealed that men were associated with an increased risk of all-cause mortality in patients with low-grade tumors (AHR 1.8, 95%CI 1.3-2.5) or patients received non-radical resection (AHR 1.6, 95%CI 1.2-2.1). Besides, in the subgroup of low-grade tumors, men were also associated with an increased risk of disease-specific mortality (AHR 2.0, 95%CI 1.2-3.3).Conclusion: Men may have worse survival after primary resection of RLPS compared with women, especially in patients with low-grade tumors or patients received non-radical resection. Gender-based disparities may deserve more attention in patients with RLPS.
Identification of a Novel Homozygous Nonsense Mutation in a Fetus with Bardet-Biedl Syndrome
Authors: Cai Meiying , Lin Min , Wang Xinrui , Su Linjuan , Wu Xiaoqing , Xie Xiaorui , Fu Xianguo , Lin Na , Xu Liangpu , Huang Hailong 
Published: November 2021

Abstract
Background: Bardet-Biedl syndrome (BBS) is a rare autosomal recessive genetic disorder with clinical and genetic heterogeneity. BBS is more commonly reported in adults and children than in fetuses. Here, we reported the intrauterine phenotype and molecular characterizations of a fetus with BBS. Methods: Chromosome karyotype analysis, whole exome sequencing (WES), and a single nucleotide polymorphism array (SNP-array) were used to analyze the genetic etiology of a fetus with enlarged kidneys, enhanced echo, and oligohydramnios. Results: The results of chromosome karyotype analysis and SNP-array on the fetus were normal. WES, however, revealed homozygous mutation of c.1177C>T (p.Arg393*) on exon 12 of the BBS1 gene, and heterozygous variation of c.2704G>A (p.Asp902Asn) on exon 22 of the CC2D2A gene. According to ACMG guidelines, c.1177C> T was identified as a pathogenic mutation and c.2704G>A was identified as an uncertain significance. Sanger sequencing showed that there was heterozygous mutation of c.1177C>T and heterozygous variation of c.2704G>A in the parents of the fetus. Conclusions: WES identified a novel homozygous nonsense mutation c.1177C>T in the BBS1 gene of a Chinese fetus. The finding provides more insight into BBS1 mutations in Asian populations in general, and provides a basis for genetic counseling.
Comparison of Simultaneous Single-position Oblique Lumbar Interbody Fusion and Percutaneous Pedicle Screw Fixation with Posterior Lumbar Interbody Fusion using O-arm Navigated C-arm free Technique for Lumbar Degenerative Diseases
Authors: Tan Ying , Tanaka Masato , Sonawane Sumeet , Uotani Koji , Oda Yoshiaki , Fujiwara Yoshihiro , Arataki Shinya , Yamauchi Taro , Takigawa Tomoyuki , Ito Yasuo 
Published: November 2021

Abstract

Background: To compare the clinical, surgical and radiographic outcomes of O-arm navigated C-arm free, simultaneous single-position oblique lumbar interbody fusion (OLIF) and percutaneous pedicle screw (PPS) fixation with minimally invasive posterior/ transforaminal lumbar interbody fusion (MI-PLIF/TLIF).Methods: This is retrospective comparative study. The study included 98 patients, 63 in single position OLIF (group SO) and 35 in MI-PLIF/TLIF (group P/T). Surgical time, blood loss, mobilization time after surgery and complications were analyzed for all patients. Clinical evaluation included visual analog scale (VAS) for back pain, oswestry disability index (ODI). Radiological parameters included cage height (CH), cage to disc ratio (CDR), DH change, pre and postoperative disc height (DH), foraminal height (FH), foraminal area (FA), segmental lordosis (SL). Results: In group SO (vs group P/T), surgical time, blood loss and mobilization time were 117.7± 34.1 minutes (171.8 ± 40.6 minutes, p<0.000001), 139.2 ± 82.0 ml (vs. 374.2 ± 247.7 ml, p<0.000001) and 2.7 ±1 .0 days (vs 3.9 ± 2.4 days, p<0.000001) respectively. The CH, CDR, DH change and postoperative DH, FH, FA increase were statistically significant in group SO compared to group P/T. VAS and ODI improvement were similar in both groups. Mobilization time is shorter in group SO. Total complication rate in group SO was 7% while that in group P/T was 11%. Conclusions: Simultaneous single position O-arm navigated C-arm free OLIF reduces the surgical time, blood loss, mobilization time after operation without the risk of an adverse event of intraoperative radiation to operating staff. Good indirect decompression can be achieved with this method. Clinical results were similar in both groups.
Lipidomics and body fat composition analysis characterises specific differences in cholesterol metabolism and steatosis between hepatitis C virus genotypes 1 and 3
Authors: Sheridan David A , Shawa Isaac Thom , Thomas E. Louise , Felmlee Daniel J. , Bridge Simon H. , Neely Dermot , Cobbold Jeremy F. , Holmes Elaine , Bassendine Margaret F. , Taylor-Robinson Simon D. 
Published: November 2021

Abstract
Background

Lipids play essential roles in the hepatitis C virus (HCV) life cycle and patients with chronic HCV infection display disordered lipid metabolism which resolves following successful anti-viral therapy. It has been proposed that HCV genotype 3 (HCV-G3) infection is an independent risk factor for hepatocellular carcinoma and evidence suggests lipogenic proteins are involved in hepatocarcinogenesis.
Aims

We aimed to characterise variation in host lipid metabolism between participants chronically infected with HCV genotype 1 (HCV-G1) and HCV-G3 to identify likely genotype-specific differences in lipid metabolism.
Methods

We combined several lipidomic approaches: analysis was performed between participants infected with HCV-G1 and HCV-G3, both in the fasting and non-fasting states, and after sustained virological response (SVR) to treatment. Sera were obtained from 112 fasting patients (25% with cirrhosis). Serum lipids were measured using standard enzymatic methods. Lathosterol and desmosterol were measured by gas-chromatography mass spectrometry (MS). For further metabolic insight on lipid metabolism, ultra-performance liquid chromatography MS was performed on all samples. A subgroup of 13 participants had whole body fat distribution determined using in vivo magnetic resonance imaging and spectroscopy. A second cohort of (non-fasting) sera were obtained from HCV Research UK for comparative analyses: 150 treatment naïve patients and 100 non-viraemic patients post-SVR.
Results

HCV-G3 patients had significantly decreased serum apoB, non-HDL cholesterol concentrations, and more hepatic steatosis than those with HCV-G1. HCV-G3 patients also had significantly decreased serum levels of lathosterol, without significant reductions in desmosterol. Lipidomic analysis showed lipid species associated with reverse cholesterol transport pathway in HCV-G3.
Conclusions

We demonstrated that compared to HCV-G1, HCV-G3 infection is characterised by low LDL cholesterol levels, with preferential suppression of cholesterol synthesis via lathosterol, associated with increasing hepatic steatosis. The genotype-specific lipid disturbances may shed light on genotypic variations in liver disease progression and promotion of hepatocellular cancer in HCV-G3.
Identification and Deletion of The Genes Responsible for Hydrogen Production in Thermoanaerobacter Ethanolicus JW200
Authors: Shao Xiongjun , Herring Christopher D. , Zhang Yuanxin , Zhang Gang , Tian Liang , Lynd Lee R. 
Published: November 2021

Abstract
Background

Thermoanaerobacter ethanolicus produces a considerable amount of ethanol from a range of carbohydrates and is an attractive candidate for applications in bioconversion processes. Due to the coupling of hydrogenase activity with fermentation product distribution, understanding hydrogen production of T. ethanolicus, particularly the genes responsible, is valuable for metabolic engineering of the species.
Results

Utilizing the hydrogenases reported in Thermoanaerobacterium saccharolyticum and Pyrococcus furiosus as templates, BLAST search identified five hydrogenase gene clusters, including two membrane-bound [NiFe] hydrogenases ech and mbh, two cytoplasmic [FeFe] hydrogenases hyd and hydII, and one cytoplasmic [NiFe] hydrogenase shi. The combined deletion of ech, mbh, shi and hydG resulted in a strain that did not produce hydrogen and showed no methyl viologen hydrogenase activity in cell extracts. Strains with deletions of all the hydrogenases except one showed normal hydrogen production. Methyl viologen hydrogenase activity was greatly reduced in all combined deletion strains except the strain with an intact hydG gene.
Conclusion

High hydrogen production and hydrogenase activities have been observed for T. ethanolicus. Five hydrogenases have been identified. Hydrogen production was eliminated by deleting genes required for all five hydrogenases. Each individual hydrogenase was verified to be capable of producing hydrogen during fermentation, indicating a high degree of redundancy and flexibility in the hydrogenase systems of T. ethanolicus. A large portion of hydrogenase activity is encoded by the [Fe-Fe] hydrogenases.