bluuebunny/crossref_metadata_2025_split huggingface dataset:
Risk Factors of Pelvic Lymph Node Metastasis and Recurrence in Patients with Early Cervical Cancer
Authors: Wang Xinmei , Zhang Hongyuan , Xu Juan , Qu Pengpeng 
Published: November 2021

Abstract
Background: Pelvic lymph node metastasis (PLNM) is one of the critical factors affecting the postoperative prognosis of patients with cervical cancer. Preoperative identification of risk factors for PLNM can optimize preoperative treatment plans and prognostic assessments.The purpose of this study was to investigate the risk factors for PLNM and its recurrence in patients undergoing radical hysterectomy for cervical cancer.Methods: Medical records of 245 patients who underwent radical hysterectomy and bilateral pelvic lymphadenectomy as primary treatment for the International Federation of Gynaecology and Obstetrics (FIGO,2009) stage IA-IIA cervical cancer between January 2010 and December 2015 were reviewed. Clinicopathological risk factors were retrospectively analyzed. All patients were followed up for 5–10 years. Multivariate analysis was performed using a logistic regression model for the analysis of risk factors for PLNM.Results: Preoperative hemoglobin level, FIGO stage, LVSI, parametrial infiltration, and tumor diameter differed significantly between the two groups (P<0.05).Multivariate analysis revealed preoperative hemoglobin <110 g/L, FIGO stage II, LVSI, parametrial infiltration, and tumor diameter ≥4 cm as significant risk factors for PLNM and recurrence of cervical cancer after surgery (P<0.05). PLNM was identified as the independent risk factor for recurrence in patients with cervical cancer after surgery (P<0.05).Conclusions: Patients with PLNM have a high recurrence rate, and postoperative follow-up should be closely followed to ensure timely detection of recurrence and treatment. For patients at high risk of PLNM, intraoperative careful and comprehensive pelvic lymph node resection should be performed to avoid missing metastatic lymph nodes and affecting the prognosis. Given the many complications of pelvic lymph node dissection for the low-risk population, further research is needed to determine whether pelvic lymphadenectomy should be attempted only in high-risk individuals.
Analysing Mental Health Problems of Adults During COVID Using a MIMIC Model: Evidence from Indian Metropolitan Cities
Authors: Husain Zakir , Ghosh Saswata , Dutta Mousumi , Chaudhuri Kausik , Chakrabarti Anindita 
Published: November 2021

Abstract
Given reduced social interactions and economic distress, mental health has emerged as an important concern during COVID-19. This study estimates the prevalence of mental health problems during the first wave of COVID and identifies its determinants among the general population of Indian metropolitan cities.The study uses a Multiple Indicators Multiple Causes model to measure depression, anxiety and stress using observed indicators of these latent constructs, and to identify the socio-economic groups at risk of these disorders. The data was collected from 1,275 adults randomly selected from the list of mobile phone users in Bengaluru, Chennai, Delhi, Kolkata, Mumbai and Hyderabad. The Depression Anxiety Stress Scale was administered to the study participants.About 46, 24 and 48 percent of respondents reported symptoms of anxiety, depression and stress, respectively. Single respondents, members of minority groups, less educated and those belonging to large households are identified to be at-risk. Results also indicate a positive relationship between economic stress and mental illness.Although COVID has increased the psychological burden of the population, all persons reporting psychological disorders may not require clinical attention. While community-based support may be sufficient in general, monitoring is required to identify at risk persons who may require clinical support.
Transcriptomic Alterations Related to Epilepsy and Tumor Suppression in the Peritumoral Area of a Rat Glioma Model
Authors: Komiyama Kazuki , Iijima Keiya , Kawabata-Iwakawa Reika , Fujihara Kazuyuki , Kakizaki Toshikazu , Yanagawa Yuchio , Yoshimoto Yuhei , Miyata Shigeo 
Published: November 2021

Abstract
Patients with glioma often demonstrate epilepsy. We previously found burst discharges in the peritumoral area in patents with malignant brain tumors during biopsy. Therefore, we hypothesized that the peritumoral area may possess an epileptic focus and that biological alterations in the peritumoral area may cause epileptic symptoms in patients with glioma. To test our hypothesis, we developed a rat model of glioma and characterized it at the cellular and molecular levels. We first labeled rat C6 glioma cells with tdTomato, a red fluorescent protein (C6-tdTomato) and implanted them into the somatosensory cortex of VGAT-Venus rats, which specifically expressed Venus, a yellow fluorescent protein in GABAergic neurons. We observed that the density of GABAergic neurons was significantly decreased in the peritumoral area of rats with glioma compared with the contralateral healthy side. By using a combination technique of laser capture microdissection and RNA sequencing(LCM-seq) of paraformaldehyde-fixed brain sections, we demonstrated that 19 genes were differentially expressed in the peritumoral area and that five of them were associated with epilepsy and neurodevelopmental disorders. In addition, the canonical pathways actively altered in the peritumoral area were predicted to cause a reduction in GABAergic neurons. These results suggest that biological alterations in the peritumoral area may be a cause of glioma-related epilepsy.
Induction of Caveolin-3/RyR2 By Liraglutide Ameliorates Diabetic Cardiomyopathy
Authors: Li Xu , Ni Zebin , Wang Junwu , Ye Xiuying , Shen Yujing , Liu Ruohai , Pan Wei , Cao Hong , Wu Wenjun 
Published: November 2021

Abstract
Background Liraglutide (LIRA), a Glucagon-like peptide-1 receptor agonist (GLP-1RA), showed potent cardioprotective effects with the mechanism remained incompletely understood. Caveolin-3 (Cav3) is the cardiomyocytes specific caveolae structural protein, decreased in the diabetic heart. Therefore, this study aimed to investigate whether LIRA exerts its effect on cardiac function in rats with type 2 diabetes mellitus (T2DM) via enhance Cav3 expression. Methods T2DM rats were used as study subjects and randomly divided into four groups: 1) CON group, 2) CON+L group, 3) DM group and 4) DM+L group. All rats received either saline or LIRA 0.2 mg/kg (by i.p injection) per day for 4 weeks. After the model was successfully established, cardiac function was determined by invasive hemodynamic evaluation methods. Immunohistochemistry and western blot were performed to understand the molecular mechanism between cardiac function and LIRA. Results Based on our results, DM group displayed higher blood glucose than Con group (20.57±2.75 mol/L vs. 4.34±0.21 mol/L), while blood glucose level in DM+L group was lower than DM group after received LIRA (10.36±1.84 mol/L). LVSP (91.39±4.98 mmHg), LV +dp/dtmax (4040.74±197.72 mmHg/s) were significantly reduced in DM group, and diabetic rats also exhibited reduced -dp/dtmax (2926.5±142.3 mmHg/s) and elevated LVEDP (10.87±0.83 mmHg). LIRA treatment showed a trend to enhance LVSP (110.76±5.61 mmHg) and ± dp/dtmax (5860.41±200.32 mmHg and 3996.8±179.3 mmHg), decreased LVEDP (7.23±0.58 mmHg). The expression of Cav3, eNOS and RyR2 was significantly decreased in the myocardium in DM group, which increased in DM+L group after LIRA administrated. Hemodynamic data showed DM rats exhibited impairment of myocardial function, while LIRA improved cardiac systolic and diastolic function, attenuate diabetic cardiomyopathy injury by improving Cav3/eNOS/NO signaling, reducing ROS level in cardiac tissues, and increasing interaction of Cav3 and ryanodine receptor 2 (RyR2). Conclusions Liraglutide ameliorates cardiac dysfunction in rats with type 2 diabetes mellitus via reducing ROS level in cardiac tissues, improving Cav3/eNOS/NO signaling and increasing interaction of Cav3 and RyR2. Keywords Type-2 diabetes Mellitus, liraglutide, caveolin-3, ryanodine receptor2, myocardial dysfunction
Dynamic analysis of detumbling a rotating satellite using flexible deceleration rod
Authors: Dai Honghua , Chen Hao , Yue Xiaokui 
Published: November 2021

Abstract
Malfunctioning satellites are generally non-cooperative tumbling objects. Due to their complex tumbling motion, it is essential to stabilize the target within an acceptable rotating range in the pre-capture phase. In contrast to contactless methods, contact methods based on flexible devices are efficient and can generate sufficient operating torque through flexible contact. However, accurate dynamic analysis of the operation is challenging because of two limitations. It is difficult to obtain a high-efficiency description of the large deformation arising from the operating process. Moreover, the contact between a flexible device and a tumbling object is hard to detect efficiently. This paper proposes a method for detumbling a free-floating rotating satellite; it uses a flexible rod to contact the solar array of the target. The absolute nodal coordinate formulation is first applied to a rod-contact detumbling model in simulation to describe the large deformation of the rod precisely with a low computational burden. Next, a two-step method to detect the contact is employed to pinpoint the contact point and speed up the simulation: coarse detection in the contactless phase and fine detection in the contact phase. Finally, the feasibility of the contact detumbling method is verified. In addition, through the further analysis of the contact process, some characteristics of this kind of strategy are studied for the first time.
Effects of Combined Abiotic Stresses On Nutrient Content of European Wheat and Implications For Nutritional Security Under Climate Change Scenarios
Authors: Galani Yamdeu , Hansen Emilie Øst , Droutsas Ioannis , Holmes Melvin , Challinor Andrew , Mikkelsen Teis , Orfila Caroline 
Published: November 2021

Abstract
Climate change is causing problems for agriculture, but the effect of combined environmental stresses on crop nutritional quality is not clear. Here we studied the effect of 10 combinations of climatic conditions (temperature, CO2, O3 and drought) in controlled growth chamber conditions on the protein and mineral content of 3 wheat varieties. Results show that wheat plants under O3 exposure alone concentrated 15-31% more grain N, Fe, Mg, Mn P and Zn, reduced K by 5%, and C did not change. Ozone in the presence of elevated CO2 and higher temperature enhanced the content of Fe, Mn, P and Zn by 2-18%. Water-limited chronic O3 exposure resulted in 9-46% higher concentrations of all the minerals, except K. The effect of climate change could increase the ability of wheat to meet adult daily dietary requirements by 1.06-1.12-fold for Fe, Zn and protein, but decrease those of Mg, Mn and P by 1.03-1.06-fold, and K by 2.78-fold. The role of wheat in future nutrition security is discussed.
Inclusive DFT Insight into Sensing Mechanism of Cyclotetrapyrole Towards Lung Irritants
Authors: Ullah Saif , Sadia Haleema , Ullah Faizan , Jadoon Tabish 
Published: November 2021

Abstract
The development of smart sensing devices for toxic analytes detection especially lung irritants is much essential. The cyclic conducting polymers having infinite 𝜋-conjugation are proved to be highly sensitive for toxic analytes. Herein, by using the DFT approach, we investigated the sensing mechanism of cyclotetrapyrole (CTPy) for accurate detection of phosgene, diphosgene, chloropicrin and chlorine at the B3LYP-D3/6-31+ G (d, p) level. The calculated interaction energies show the physisorption of lungs irritants over the CTPy surface. Natural bond orbital (NBO) and charge decomposition (CDA) analyses predict charge transfer interactions in the complexes. The reduced density gradient (RDG) approach reveals that charge transfer electrostatic hydrogen bonding interactions dominate in the complexes. The sensitivity of CTPy towards lung irritants is further illustrated by the reduction in HOMO-LUMO energy gaps, red shifting of \({{\lambda }}_{\text{m}\text{a}\text{x}}\) in UV–Visible specta. Density of state (DOS) analysis affirm that enhanced conductivity upon complexation is due to the origination of new energy states in occupied and virtual orbitals nearer to the Fermi level. Moreover, PDOS spectra show that CTPy primarily contributes to the energy of HOMO. The outcome of the current study depicts appreciable sensitivity of CTPy towards lung irritants. We believe that the upshot of the current findings and their forecasts will provide useful guidelines for an experimentalist to design highly sensitive sensors for toxic analytes using CTPy.
Comparative Analysis of Three Pairs of Surrogate Redox Partners for in Vitro Activity Reconstitution of Class I Cytochrome P450 Enzymes
Authors: Liu Xiaohui , Li Fengwei , Sun Tianjian , Guo Jiawei , Zhang Xingwang , Zheng Xianliang , Du Lei , Zhang Wei , Ma Li , Li Shengying 
Published: November 2021

Abstract
Cytochrome P450 enzymes (P450s) are highly attractive biocatalysts due to their versatile catalytic activities. A vast majority of P450s require redox partner (RP) proteins to sequentially transfer two electrons for O2 activation and substrate oxidation. However, little information is available on cognate RPs for P450s, which greatly limits P450 function exploration and practical application. Thus, the stategy of building various hybrid P450 catalytic systems with surrogate RPs has often adopted to engineer P450 biocatalysts for different purposes. In this study, we comprehensively compare three pairs of frequently-used surrogate redox partners SelFdx1499/SelFdR0978, Adx/AdR and Pdx/PdR and in terms of their electron transfer properties. The three selected bacterial Class I P450s to accept electrons from RPs include PikC, P450sca-2 and CYP-sb21, which are responsible for production of macrolide antibiotics, the cholesterol-lowering drug pravastatin, and a hair-growth-stimulating agent. Both experimental studies and structural analysis show that SelFdx1499/SelFdR0978 is the most promising RP compared to Adx/AdR and Pdx/PdR. The results provide insights into the domination for P450-redox partner interactions in modulating the catalytic activity of P450s. This study not only produces a more active biocatalyst but also suggests a general chose for a universal reductase which would facilitate engineering of P450 catalyst.
Visual Reaction Time: A Tool for Reducing Road Traffic Collisions and Driving Simulator.
Authors: Rajdeep Prashant , Patel Lajja , CD Steffy , Panchal Preeti 
Published: November 2021

Abstract
Objective- Attenuating post lockdown vehicular speed by employing visual reaction time as a tool to prime the citizens for creating decorum of driving and checking the road traffic fatalities.Background- It is indispensable to curb the driving speed post lockdown to avoid accidents. Even though, the impact of inactivity on RT has been well established, an insight into the new method can deal with the gross issue of road traffic casualty worldwide. Method- Using a web-based platform (http://physicsiology.com), quantification of post lockdown speed was achieved for 643 participants under average speed before lockdown and RT measurement. Results- Compared to pre lockdown vehicular speed, reduced post lockdown speed was well calculated and suggested. Also, there was a correlation between RT, age, and days of lockdown.Conclusions- Containment of speed can be achieved to prime people through RT. Additionally, RT can determine the rate of change of frequency (ROCOF) for detecting the swiftness of action (i.e., the brain's ability to deal with the transition between reaction times of different events) required for averting road traffic collisions. Compelling to suggest a need for a humanoid simulator that can garner real-time data.Application- Suggesting a fresh outlook for designing a contraption for a better appraisal of the fleet in driving skills, thus beaconing the course towards restraining road traffic fatalities
Modelling the Potential Acute and Post-Acute Burden of COVID-19 Under the Australian Border Re-Opening Plan
Authors: Angeles Mary Rose , Dona Sithara Wanni Arachchige , Nguyen Dieu , Le Long , Hensher Martin 
Published: November 2021

Abstract
Background
Concerns have grown that post-acute sequelae of COVID-19 may affect significant numbers of survivors. However, the analyses used to guide policy-making for Australia’s national and state re-opening plans have not incorporated non-acute illness in their modelling. We therefore develop a model by which to estimate the potential acute and post-acute COVID-19 burden using disability-adjusted life years (DALYs) associated with the re-opening of Australian borders and the easing of other public health measures, with particular attention to longer term, post-acute consequences and the potential impact of permanent functional impairment following COVID-19.
Methods
A model was developed to estimate the burden of COVID-19 using DALYs. It was then applied to different scenarios drawn from the Doherty Institute’s modelling report, to estimate the likely DALY losses under the Australian national reopening plan. Uncertainty and sensitivity analysis were performed to examine the robustness of the results.
Results
Mortality was responsible for 72%-74% of the total base case COVID-19 burden. Long COVID and post-intensive care syndrome accounted for at least 19% and 3% of the total base case DALYs respectively. When included in the analysis, permanent impairment could contribute up to 51%-55% of total DALYs lost.
Conclusions
The impact of Long COVID and potential long-term post-COVID disabilities could contribute substantially to COVID-19 burden in Australia’s post-vaccination setting. As vaccination coverage increases, the share of COVID-19 burden driven by longer-term morbidity rises relative to mortality. As Australia re-opens, better estimates of COVID-19 burden can assist with decision-making on pandemic control measures and planning for the healthcare needs of COVID-19 survivors. Our estimates highlight the importance of valuing the morbidity of post-COVID-19 sequelae, above and beyond simple mortality and case statistics.
Clinicopathological and Ultrasound Features as Risk Stratification Predictors of Clinical and Pathological Nodal Status in Papillary Thyroid Carcinoma: A Study of 748 Patients
Authors: ZHANG Cui , LI Baojun , ZHANG LEI , CHEN Fengjiao , ZHANG Yanhua , CHENG Wen 
Published: November 2021

Abstract
Background: Papillary thyroid carcinoma (PTC) is the most common histological type of thyroid malignancy that tends to metastasize to cervical lymph nodes. In the present study, we aimed to investigate which clinicopathologic and ultrasound features of PTC are associated with clinical lymph node metastasis (LNM) and numbers of pathological LNM.Methods: From January 2016 to December 2018, we identified a cohort of patients with PTC who underwent cervical ultrasonography and were diagnosed through operation and pathology. Clinical N1(cN1) and >5 pathologic N1(pN1) was performed in accordance with a standardized protocol. A model to determine the intermediate risk stratification of the 2015 ATA was established using the outcomes of univariate and multivariate analyses. Results: we collected 748 PTC patients in the final inclusion criteria. From the analyses, primary tumor size >21mm, capsule contact, extrathyroidal extensions (ETE), postsurgical radioiodine (RAI) treatment and central LNM remained independent risk factors for cN1 in PTC patients. Multifocality, primary tumor size >21mm, capsule contact, ETE and RAI treatment are significant independent risk factors for >5 pN1. Primary tumor size >21mm, capsule contact, ETE and RAI treatment were independent risk factors for both cN1 and >5 pN1.Conclusions: We conclude that primary tumor size >21mm, capsule contact, ETE and RAI treatment were independent predictors of for intermediate risk of recurrence in patients with PTC. Ultrasonography is a good technique for the preoperative lymph node staging of PTC and is helpful for detecting LNM.
Characterization of Ribulose-1,5-Bisphosphate Carboxylase-Oxygenase Activase (Rca) Genes in Durum Wheat
Authors: Nigro Domenica , Giove Stefania L. , Colasuonno Pasqualina , de Pinto Roberta , Marcotuli Ilaria , Gadaleta Agata 
Published: November 2021

Abstract
Durum wheat is one of the most widely cultivated cereal crop in the Mediterranean area. Its production has been triggered by drought and rising temperature, both affecting the photosynthetic machinery. Rubisco is one of the most important enzymes in plants. Despite its major role in the control of carbon cycle it has a very low efficiency, which is restored by the action of Ribulose-1,5-bisphosphate carboxylase/oxygenase activase (Rca), a protein belonging to the AAA+ family. The main objective of our work was to isolate and characterize Rca genes in durum wheat and determine their phylogeny with other main crops and model species. Besides a genetic and physical position of Rca1 gene was allowed in a RIL mapping population previously developed. In silico analysis, performed in order to understand whether Rca1 gene was differentially expressed under stress condition, highlighted that homoeologous Rca1 genes have different expression levels especially after infections by Zymoseptoria, powdrey mildew and fusarium. A deeper knowledge of Rca genes structures as well as a better understanding of their physiological role in durum wheat might be of greater importance in panning future modern breeding programs to improve crop yield in adverse environmental condition.
The Effect of Functional Mandibular Advancement for Adolecent Patients with Skeletal Class Ⅱ Malocclusion on the TMJ——a Systematic Review and Meta-Analysis
Authors: Ding Lan , Chen Rui , Liu Jiaxin , Wang Yuan , Chang Qian , Ren Liling 
Published: November 2021

Abstract
Objectives: This study aimed to assess whether functional mandibular advancement will induce or aggravate temporomandibular joint disorders.Methods: All searched databases, including PubMed, Web of Science, EMBASE, Cochrane Central Register of Controlled Trails were searched. Gray literature and unpublished literature was also searched. Randomized controlled trails directly observe the condition of patients’ TMJ after finishing treatment will be considered to include in our study. Cochrane Collaboration risk of bias tool was used to assess the quality of included studies according to the established inclusion criteria.Result:Finally 13 researches were statistically pooled in meta-analysis. The number of samples under investigated among primary studies was 285 individuals ,there were 142 patients who developed temporomandibular symptoms during or after treatment. But all the subjective symptoms disappeared during follow-up time. The statistical outcomes proved that patients recieved FMA didn’t show more tendency to develop temporomandibular symptoms[I²=25%, OR=0.39, 95%CI (0.22 ,0.68), p=0.001].Conclusion: (1) TMJ symptoms may occur during the functional oral appliance wearing, but the symptoms will release or disappear during follow-up period. (2) Less convinced evidence indicate that slightly previous TMD will be improved after treatment. (3) There is TMJ disc anterior displacement observed during treatment, but most of them will return to the normal position later. (4) Moderate evidence support that FMA will not induce or aggravate TMD.