bluuebunny/crossref_metadata_2025_split huggingface dataset:
Different Electrophysiological Responses to Pain-Related Visual Stimuli Between Fibromyalgia and Chronic low Back Pain Women: A Pilot Case-Control Study
Authors: Lai Carlo , Ciacchella Chiara , Pellicano Gaia Romana , Altavilla Daniela , Sambucini Daniela , Paolucci Teresa , Sorgi Maria Laura , Di Franco Manuela , Saggini Raoul , Aceto Paola 
Published: December 2021

Background Fibromyalgia is a chronic pain syndrome which occurs in the absence of an organic damage, whom causes is still unclear. Aims of this pilot study were to investigate the neural correlates of fibromyalgia in response to pain-related visual stimuli and explore the psychological differences among fibromyalgia, chronic low back pain (CLBP) and healthy conditions. Methods After a clinical assessment, electrophysiological responses to pain-related visual stimuli were recorded using a 256-Hydrocel Geodesic-Sensor-Net. Event-related potentials (ERPs), standardised low-resolution electromagnetic tomography (sLORETA), and psychological (Symptom Checklist-90-Revised) data were analysed for a total sample of 23 women (5 healthy volunteers, 12 fibromyalgia patients, 6 CLBP patients). Results The main finding was that fibromyalgia women reported a different brain response to pain-related visual stimuli on the frontal montage compared to women with CLBP ( p = .028). Moreover, fibromyalgia women showed an increased activity mainly on the hippocampus ( p = .003) and the posterior cingulate cortex ( p ≤ .001) in response to algic stimuli compared to not algic ones. Lastly, these women presented higher scores on the somatization ( p = .002), obsession-compulsion ( p = .045), depression ( p = .043) and positive symptom distress ( p = .023) dimensions compared to the healthy women. Conclusions These preliminary results suggest that although the painful symptoms are similar, the central elaboration of pain could be different between women with fibromyalgia and those with CLBP. Moreover, these findings provide preliminary evidences about the great alert and the central sensitivity to pain-related information regarding fibromyalgia patients.
Neutrophil-to-lymphocyte ratio, blood eosinophils and COPD exacerbations: a cohort study
Authors: Ellingsen Jens , Janson Christer , Bröms Kristina , Lisspers Karin , Ställberg Björn , Högman Marieann , Malinovschi Andrei 
Published: December 2021

BackgroundBlood neutrophil-to-lymphocyte ratio (NLR) and blood eosinophils (B-Eos) are emerging biomarkers in COPD. This study examined whether they could predict acute exacerbations of COPD (AECOPDs), and determined their longitudinal stability.MethodsIn this closed cohort study, Swedish subjects with spirometry-verified COPD attended three yearly visits in a stable phase of the disease. Blood cell counts, spirometry and questionnaire-assessed AECOPD-history (worsening of COPD leading to an unscheduled visit and/or use of antibiotics and/or oral corticosteroids) were collected at each visit.ResultsOf 466 included subjects 57% were female. Baseline mean±sdforced expiratory volume in 1 s was 58±17% predicted. High NLR (≥3.0) was more common in subjects with previous AECOPDs than in those without (33.5%versus20.4%, p=0.002). In two-level mixed-effects logistic regression models adjusted for confounders, NLR as a continuous variable (OR 1.20, 95% CI 1.04–1.38) and B-Eos ≥300 cells·µL−1(OR 1.54, 95% CI 1.06–2.24) were associated with future AECOPDs. In 386 subjects with blood cell data available at all three visits, the intraclass correlation coefficient for NLR was 0.61 (95% CI 0.56–0.66) and for B-Eos 0.69 (95% CI 0.64–0.73). NLR was persistently ≥3.0 in 10.6% and B-Eos was persistently ≥300 cells·µL−1in 15.3%.ConclusionsStable phase NLR and B-Eos were associated with future AECOPDs. NLR on its own is probably not useful to predict AECOPDs but might be included in a risk scoring index. A minority of subjects with COPD had persistently elevated stable-phase NLR or B-Eos, and the biomarkers showed fair longitudinal reliability.
Identification of proteins associated with bast fiber growth of ramie by differential proteomic analysis
Authors: Li Fu , Zeng Zheng , Huang Renyan , Wang Yanzhou , Liu Touming 
Published: December 2021

Abstract
Background
Ramie is an important fiber-producing crop in China, and its fibers are widely used as textile materials. Fibers contain specialized secondary cellular walls that are mainly composed of cellulose, hemicelluloses, and lignin. Understanding the mechanism underlying the secondary wall biosynthesis of fibers will benefit the improvement of fiber yield and quality in ramie.

Results
Here, we performed a proteomic analysis of the bark from the top and middle parts of the stem, where fiber growth is at different stages. We identified 6971 non-redundant proteins from bast bark. Proteomic comparison revealed 983 proteins with differential expression between the two bark types. Of these 983 proteins, 46 were identified as the homolog of known secondary wall biosynthetic proteins of Arabidopsis, indicating that they were potentially associated with fiber growth. Then, we proposed a molecular model for the secondary wall biosynthesis of ramie fiber. Furthermore, interaction analysis of 46 candidate proteins revealed two interacting networks that consisted of eight cellulose biosynthetic enzymes and seven lignin biosynthetic proteins, respectively.

Conclusion
This study sheds light on the proteomic basis underlying bast fiber growth in ramie, and the identification of many candidates associated with fiber growth provides important basis for understanding the fiber growth in this crop.
Immune response to hepatitis B vaccine following complete immunization of children attending two regional hospitals in the Southwest region of Cameroon: a cross sectional study
Authors: Anutebeh Ephesians N. , Tatah Lambed , Feteh Vitalis F. , Aroke Desmond , Assob Jules C. N. , Choukem Simeon Pierre 
Published: December 2021

Abstract
Background
Hepatitis B virus (HBV) infection despite being a vaccine preventable disease remains a global public health problem. In Cameroon, the hepatitis B vaccine was introduced in the expanded program on immunisation in 2005, but there has been limited evaluation of the HBV surface antibody response post vaccination.

Objective
We investigated the immune response to hepatitis B vaccine in infants who received the DPT-Hep B-Hib vaccine, and we assessed HBsAg carriage in non-responders. We also investigated factors associated with non-response or poor response.

Methods
Using a hospital based cross sectional design and a structured questionnaire over a four-month period (January to April 2019), we collected data to determine factors associated with hepatitis B surface antibody (anti-HBs) response from infants aged 6 to 9 months attending infant welfare clinics (IWC) at the Buea and Limbe regional hospitals. We collected venous blood and measured anti-HBs titres using a quantitative Foresight® ELISA. We entered and analysed data using EpiData version 3.1 and SPSS version 25 respectively.

Results
Of the 161 infants enrolled, 159 (98.8%) developed anti-HBs antibodies. Of these 159, 157 (97.5%) and 117 (72.7%) developed ≥ 10.0 mIU/ml (seroprotection) and ≥ 100.0 mIU/ml anti-HBs titres respectively. Being younger (6 months old) was associated with seroprotection (Cramer V = 0.322, p = 0.001). Spearman rho’s relational analysis showed that immunity against HBV reduced as the duration since the last dose increased (r = −0.172; P = 0.029). However, a Firth logistic regression showed no significant association of factors with inadequate immunity. All 12 (7.5%) infants exposed to HBV at birth, received the hepatitis B vaccine at birth, including four who received HBIG, and all were protected. Four infants (2.5%) had anti-HBs titres < 10.0 mIU/mL (non-responders) but had no peculiarity.

Conclusion
The seroprotective rate following hepatitis B vaccination of infants is high even in exposed infants. Our study suggests that Cameroon’s HBV vaccine in the Expanded Program on Immunisation (EPI) is effective against HBV, although we could not account for the 2.5% non-response rate. Large scale studies are needed to further explore non-response to the vaccine.
A survey of nursing teachers’ awareness of discrimination and inequity in telephone nursing care
Authors: Holmström Inger K , Kaminsky Elenor , Höglund Anna T , Carlsson Marianne 
Published: December 2021

Abstract
Background
Nursing care should be respectful of and unrestricted by patients’ age, ethnicity, gender, dis/abilities or social status, and such values should be taught to nursing students. Nursing teachers are crucial as role models, and their values are essential. In telephone nursing, only age, sex and ethnicity are known to the registered nurses, which can be challenging. The aim of this study was to explore awareness of discrimination and inequity in telephone nursing among nursing teachers.

Methods
A study specific survey was filled in by 135 nursing teachers from three universities in Sweden. The survey included short descriptions of 12 fictive persons who differed in age, ethnicity and sex and with questions about their estimated life situation. The teachers’ estimations of life situation were ranked from lowest probability to highest probability. A ‘good life index’ was constructed and calculated for each fictive person. It included quality of life, power over one’s own life and experience of discrimination.

Results
The results indicate that the nursing teachers were aware of how power and age, ethnicity and sex are related; that is, they were aware of discrimination and inequity in healthcare. The persons assessed to be most likely to lead a good life were males of Swedish origin, followed by females of Swedish origin. Persons with non-European origin were estimated to have the highest probability of experiencing discrimination.

Conclusions
The nursing teachers were aware of discrimination and inequity in healthcare. They were able to estimate a fictive person’s life situation based on the limited knowledge of age, ethnicity and sex. This is important, as their values are pivotal in theoretical and practical nursing education.
Antibiotic resistance modifying ability of phytoextracts in anthrax biological agent Bacillus anthracis and emerging superbugs: a review of synergistic mechanisms
Authors: Dassanayake Mackingsley Kushan , Khoo Teng-Jin , An Jia 
Published: December 2021

AbstractBackground and objectivesThe chemotherapeutic management of infections has become challenging due to the global emergence of antibiotic resistant pathogenic bacteria. The recent expansion of studies on plant-derived natural products has lead to the discovery of a plethora of phytochemicals with the potential to combat bacterial drug resistance via various mechanisms of action. This review paper summarizes the primary antibiotic resistance mechanisms of bacteria and also discusses the antibiotic-potentiating ability of phytoextracts and various classes of isolated phytochemicals in reversing antibiotic resistance in anthrax agentBacillus anthracisand emerging superbug bacteria.MethodsGrowth inhibitory indices and fractional inhibitory concentration index were applied to evaluate the in vitro synergistic activity of phytoextract-antibiotic combinations in general.FindingsA number of studies have indicated that plant-derived natural compounds are capable of significantly reducing the minimum inhibitory concentration of standard antibiotics by altering drug-resistance mechanisms ofB. anthracisand other superbug infection causing bacteria. Phytochemical compounds allicin, oleanolic acid, epigallocatechin gallate and curcumin andJatropha curcasextracts were exceptional synergistic potentiators of various standard antibiotics.ConclusionConsidering these facts, phytochemicals represents a valuable and novel source of bioactive compounds with potent antibiotic synergism to modulate bacterial drug-resistance.
Epidemiology and demographics of juvenile idiopathic arthritis in Africa and Middle East
Authors: Al-Mayouf Sulaiman M. , Al Mutairi Muna , Bouayed Kenza , Habjoka Sara , Hadef Djohra , Lotfy Hala M. , Scott Cristiaan , Sharif Elsadeg M. , Tahoun Nouran 
Published: December 2021

AbstractJuvenile Idiopathic Arthritis (JIA) is a group of chronic heterogenous disorders that manifests as joint inflammation in patients aged <16 years. Globally, approximately 3 million children and young adults are suffering from JIA with prevalence rates consistently higher in girls. The region of Africa and Middle East constitute a diverse group of ethnicities, socioeconomic conditions, and climates which influence the prevalence of JIA. There are only a few studies published on epidemiology of JIA in the region. There is an evident paucity of adequate and latest data from the region. This review summarizes the available data on the prevalence of JIA and its subtypes in Africa and Middle East and discusses unmet needs for patients in this region. A total of 8 journal publications were identified concerning epidemiology and 42 articles describing JIA subtypes from Africa and Middle East were included. The prevalence of JIA in Africa and Middle East was observed to be towards the lower range of the global estimate. We observed that the most prevalent subtype in the region was oligoarticular arthritis. The incidence of uveitis and anti-nuclear antibody (ANA) positivity were found to be lower as compared to the incidence from other regions. There is a huge unmet medical need in the region for reliable epidemiological data, disease awareness, having regional and local treatment guidelines and timely diagnosis. Paucity of the pediatric rheumatologists and economic disparities also contribute to the challenges regarding the management of JIA.
Shen-Zhi-Ling oral liquid ameliorates cerebral glucose metabolism disorder in early AD via insulin signal transduction pathway in vivo and in vitro
Authors: Qin Gaofeng , Dong Yunfang , Liu Zhenhong , Gong Zhuoyan , Gao Chenyan , Zheng Mingcui , Tian Meijing , He Yannan , Zhong Liqun , Wang Pengwen 
Published: December 2021

Abstract
Background
Shen-Zhi-Ling oral liquid (SZL) is an herbal formula known for its efficacy of nourishing “heart and spleen”, and is used for the treatment and prevention of middle- and early-stage dementia. This study investigated the effects of SZL on amelioration of AD, and examined whether the underlying mechanisms from the perspective of neuroprotection are related to brain glucose metabolism.

Methods
Firstly, LC–MS/MS was used to analysis the SZL mainly enters the blood component. Then, the effects of SZL on cognitive and behavioral ability of APP/PS1 double transgenic mice and amyloid protein characteristic pathological changes were investigated by behavioral study and morphological observation. The effects of SZL on the ultrastructure of mitochondria, astrocytes, and micrangium related to cerebral glucose metabolism were observed using transmission electron microscopy. Then, micro-PET was also used to observe the effects of SZL on glucose uptake. Furthermore, the effects of SZL on insulin signaling pathway InR/PI3K/Akt and glucose transporters (GLUT1 and GLUT3) were observed by immunohistochemistry, Western-blot and RT-qPCR. Finally, the effects of SZL on brain glucose metabolism and key enzyme were observed. In vitro, the use of PI3K and/or GSK3β inhibitor to observe the effects of SZL drug-containing serum on GLUT1 and GLUT3.

Results
In vivo, SZL could significantly ameliorate cognitive deficits, retarded the pathological damage, including neuronal degeneration, Aβ peptide aggregation, and ultrastructural damage of hippocampal neurons, improve the glucose uptake, transporters and glucolysis. Beyond that, SZL regulates the insulin signal transduction pathway the insulin signal transduction pathway InR/PI3K/Akt. Furthermore, 15% SZL drug-containing serum increased Aβ42-induced insulin signal transduction-pathway related indicators and GLUT1 and GLUT3 expression in SH-SY5Y cells. The improvement of GLUT1 and GLUT3 in the downstream PI3K/Akt/GSK3β signaling pathway was reversed by the use of PI3K and/or GSK3β inhibitor.

Conclusions
In summary, our results demonstrated that improving glucose uptake, transport, and glycolysis in the brain may underlie the neuroprotective effects of SZL, and its potential molecular mechanism may be related to regulate the insulin signal transduction pathway.
The influence of climate change on food innovation technology: review on topical developments and legal framework
Authors: Ukhurebor Kingsley Eghonghon , Aidonojie Paul Atagamen 
Published: December 2021

AbstractThe influence of climate change on agriculture, especially as it relates to the production of food, changes with reverence to duration and space, of which most of these influences are diverse and remarkably uncertain. Undoubtedly, the application of food innovation technology (FIT) in the agricultural processes is an important response for operative and objective adaptation and mitigation of climate change. Consequently, there is a need to urgently re-evaluate the procedures for FIT so as to address the diversities and uncertainties ensuing from these influences of climate change on agriculture with the aim of improving the production of food. Therefore, the application of climate-smart agricultural (CSA) activities with resilience in agricultural events as well as more aids in the application of resources for both in the adaptation and mitigation of climate change by means of FIT will be of great assistance in this regard. Hence, this study presents a facile review of some of the topical developments in the production of food with reverence to the influence of climate change on FIT. Some legal frame-work on climate change with respect to FIT are also been discussed.
Effect of various additives on aluminum oxide thin films prepared by dip coating, thermal behavior, kinetics and optical properties
Authors: Bouzbib Mohammed , El Marouani Maryam , Sinkó Katalin 
Published: December 2021

AbstractAluminum oxide thin films attract research interest due to their properties. Aluminum acetate was used as an Al source with acetic acid, oxalic acid, and nitric acid as additives. The transmittance and the thickness of the films strongly depend on the additives, with the approximate bandgap energy changing from 5 ev to 5.4 ev. The aluminum oxide film deposited by dip-coating is presented great uniform surface morphology. The knowledge of the degradation kinetics of materials is essential for investigating the thermal stability of compounds. The acetic acid thin film proved to be the most efficient additive by demonstrating interesting optoelectronic properties. The thin films deposited by dip-coating were characterized by using X-ray grazing incidence diffraction, SEM, UV-Visible spectroscopy. Gamma aluminum oxide thin films prepared by acetic acid can be a good candidate for a wide range of optical applications.
Identification of body fluids—menstrual blood, saliva, and nasal secretions—over different periods of time, using mRNA
Authors: Hussein Riham F. , El Mahdy Sherif Mohamed , Saged Nashwa Mohammed , Rashed L. , Ghaleb Sherien S. 
Published: December 2021

Abstract
Background
Forensic examination of biological samples started at the beginning of the twentieth century by applying the ABO blood group system in evidence related to crimes or human identification. In the present study, real-time PCR multiplex was used to identify dried and stored swabs (saliva, nasal secretions, and menstrual blood) through the target genes of saliva (histatin 3 and statherin), nasal secretions (statherin and BPIFA1), and menstrual blood (metalloproteinases 10 and 7).

Results
The expressions of histatin 3 and statherin in the dried saliva decreased over days of storage with a significant p value of <0.001. BPIFA1 was highly expressed in nasal secretions, and the expression level significantly decreased throughout the study with a significant p value of <0.001. The MMP7 and MMP10 genes were highly expressed in the menstrual blood, and the expression level decreased over days of storage with a significant p value of p<0.001.

Conclusions
Dried swabs of the saliva, Nasal secretions, Menstrual blood can be identified over the storage duration of the study using mRNA profiling of specific markers.
Coxiella burnetti prosthetic joint infection in an immunocompromised woman: iterative surgeries, prolonged ofloxacin-rifampin treatment and complex reconstruction were needed for the cure
Authors: Miailhes Patrick , Conrad Anne , Sobas Chantal , Laurent Frederic , Lustig Sebastien , Ferry Tristan ,   on behalf of the Lyon BJI study group, Ferry Tristan , Valour Florent , Perpoint Thomas , Ader Florence , Roux Sandrine , Becker Agathe , Triffault-Fillit Claire , Conrad Anne , Pouderoux Cécile , Chauvelot Pierre , Chabert Paul , Lippman Johanna , Braun Evelyne , Lustig Sébastien , Servien Elvire , Batailler Cécile , Gunst Stanislas , Schmidt Axel , Sappey-Marinier Elliot , Ode Quentin , Fessy Michel-Henry , Viste Anthony , Besse Jean-Luc , Chaudier Philippe , Louboutin Lucie , Van Haecke Adrien , Mercier Marcelle , Belgaid Vincent , Gazarian Aram , Walch Arnaud , Bertani Antoine , Rongieras Frédéric , Martres Sébastien , Trouillet Franck , Barrey Cédric , Mojallal Ali , Brosset Sophie , Hanriat Camille , Person Hélène , Céruse Philippe , Fuchsmann Carine , Gleizal Arnaud , Aubrun Frédéric , Dziadzko Mikhail , Macabéo Caroline , Patrascu Dana , Laurent Frederic , Beraud Laetitia , Roussel-Gaillard Tiphaine , Dupieux Céline , Kolenda Camille , Josse Jérôme , Craighero Fabien , Boussel Loic , Pialat Jean-Baptiste , Morelec Isabelle , Tod Michel , Gagnieu Marie-Claude , Goutelle Sylvain , Mabrut Eugénie 
Published: December 2021

Abstract
Background
Q fever is a zoonotic disease caused by the bacterium Coxiella burnetii, a strictly intracellular pathogen that can cause acute and chronic infection. Chronic Q fever can occur in immunocompetent as well as in immuno-compromised hosts, as a persistent localized infection. The main localizations are endocardial, vascular and, less frequently, osteoarticular. The most frequent osteoarticular form is spondyliscitis. Recommended treatment is combined doxycycline and hydroxychloroquine for 18 months, with cotrimoxazole as another option. Coxiella burnetti infection has been implicated in rare cases of prosthetic joint infection (PJI), and the medical and surgical management and outcome in such cases have been little reported.

Case presentation
We report an unusual case of chronic Q fever involving a hip arthroplasty in an immunocompromised woman treated with tumor necrosis factor (TNF)-α blockers for rheumatoid arthritis. Numerous surgical procedures (explantation, “second look”, femoral resection and revision by megaprosthesis), modification of the immunosuppressant therapy and switch from doxycycline-hydroxychloroquine to prolonged ofloxacin-rifampin combination therapy were needed to achieve reconstruction and treat the PJI, with a follow-up of 7 years.

Conclusions
Coxiella burnetti PJI is a complex infection that requires dedicated management in an experienced reference center. Combined use of ofloxacin-rifampin can be effective.