bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Serum of MEF2D Expression in Patients with Myocardial Infarction, May be Clinical Indicators and it Mediated ROS Signaling by HDAC5
Authors: Feng Tao , Hu Yongyi , Deng Zhihua 
Published: January 2021

Abstract
Objectives: In this study, we explain that the function of MEF2D gene in myocardial infarction and its possible mechanism. Methods: Patients with MI and normal volunteers were collected. The left anterior descending arteries (LAD) of mice were ligated to induce myocardial infarction. H9c2 cells were stimulated with 5% oxygen (O2) and 5% carbon dioxide (CO2) and 90% N2 for 24 h. Quantitative polymerase chain reaction (qPCR), Microarray experiments, Western blot, Enzyme-linked immunosorbent assay (ELISA) and Immunofluorescent staining were used at this experiment. Results: MEF2D mRNA and protein expressions in heart tissue of patients and mice with myocardial infarction were reduced. MEF2D protein prevented Myocardial infarction in mice of myocardial infarction. The inhibition of MEF2D aggravated Myocardial infarction in mice of myocardial infarction. MEF2D gene promoted ROS-induced oxidative stress in vitro model of myocardial infarction. MEF2D interlinkage HDAC5 to reduced oxidative stress in vivo model or vitro model. The regulation of HDAC5 adjusted the function of MEF2D in vitro model. Discussion: These data confirmed that the serum of MEF2D expression in patients with Myocardial infarction was reduced, may be clinical indicators and it inhibited ROS-induced oxidative stress signaling by HDAC5. Thus, our results suggest that targeting MEF2D may provide an approach for the treatment of myocardial infarction.
Premature Ovarian Insufficiency After Bone Marrow Transplantation for Thalassemia Major in a Patient With Primary Amenorrhea and 46, XY Karyotype: a Case Report and Literature Review
Authors: Li Hui , Li Jin , Li Xiaohong , Yi Hong , Ren Qixiu , Chen Xiaoyan 
Published: January 2021

Abstract
Background: Iatrogenic factor is one of the recognized causes for premature ovarian insufficiency. The aim of this case report was to present a rare case with premature ovarian insufficiency and 46, XY karyotype after bone marrow transplant for thalassaemia major at childhood. We also reviewed some relevant literature in this report.Case presentation: A 17-year-old girl was presented with primary amenorrhea and premature ovarian insufficiency after receiving chemotherapy and bone marrow therapy from her brother due to thalassaemia major at childhood. She had poor secondary sex characteristics, assessed as stage I for the development of breasts and external genitalia based on the Tanner scale. Transabdominal ultrasound showed small uterus with visible endometrial lining and small ovaries. Laboratory data showed hypergonadotropic hypogonadism profile with low level of estrogen and high level of follicular-stimulating hormone (FSH). Patient’s peripheral lymphocytes karyotype was 46, XY. Conclusions: A correct diagnosis is not only dependent on clinical manifestations, but also the detailed medical history. In light of the relatively high prevalence of thalassemia, fertility preservation should be considered for young girls before they receive chemotherapy, radiation treatment and bone marrow transplant.
Exploring Experiences of Implementing Standardized Cancer Patient Pathways Within Investigatory Units – a Qualitative Study
Authors: Winterling Jeanette , Delilovic Sara , Dervish Jessica , Gunnarsson Malin , Åhström Mårten , Hasson Henna 
Published: January 2021

Abstract
Background: In the implementation of standardized cancer patient pathways (CPPs), the investigatory units, endoscopy, radiology and pathology, are crucial to ensure an eventual cancer diagnosis. However, when evaluating the implementation of CPPs, little attention has been paid to the healthcare professionals working in these units. The aim of this study was to explore experiences of the implementation of CPPs among health professionals in investigatory units.Methods: This descriptive qualitative study included 55 health professionals working in investigatory units. Participants were interviewed in 2017–2018, and data were analysed using thematic analysis. Results: The health professionals reported benefits, facilitators and challenges when describing their experiences of implementing CPPs. Benefits included that that CPP improved collaboration and increased focus on the patients. Facilitators in the implementation process included pre-existing well-functioning work processes and having supportive functions (e.g. coordinators). Challenges included the lack of staff and clinical equipment, as well as unjustified time-slots and incorrect referrals.Conclusions: The findings show that most health professionals working in investigatory units’ experience benefits with the implementation of CPP, but the lack of resources was especially hard to overcome.
Accuracy of Digital Templating for Non-Cemented Total Hip Arthroplasty: A Large Series by a Single Surgeon
Authors: Wheeler Adam , Gradisar Ian M. 
Published: January 2021

Abstract
IntroductionPreoperative planning for total hip arthroplasty (THA) is an essential step taken to reduce surgical errors and improve patient outcomes. Previously, templating using acetate radiographs proved accurate and beneficial. However, digital templating of digitally acquired radiographs has become more commonplace. The purpose of this study was to quantify accuracy of digital templating for THA’s performed by a single surgeon over a 4-year period and to determine if accuracy improved over time. Materials and Methods491 patients undergoing primary THA from 2013-2017 performed by a single surgeon were retrospectively analyzed. Digital templating was performed using ORTHOVIEW Orthopaedic Digital Templating for CARESTREAM PACS (© 2019 Carestream Health). Digitally templated acetabular and femoral component sizes were compared to actual implanted sizes to determine template percent accuracy. To investigate changes in accuracy over time, THA cases were divided in half and compared. Chi Square and Fisher’s Exact Tests were used to determine statistically significant differences in percent accuracy over time. Results Overall percent accuracy of acetabular and femoral components was 97.4% and 87.2% respectively. When comparing the first and second half, percent accuracy of acetabular and femoral components was 98.4% versus 97.1% (P =0.58) and 89.4% versus 84.9% (P= 0.14) respectively. ConclusionsThis study represents one of the largest retrospective analyses aimed at determining accuracy of digital templating in THA procedures performed by a single surgeon at a single institution. Percent accuracy was consistently high throughout time and deemed an accurate methodology for preoperative planning of cementless THA.
HuR-mediated posttranscriptional modification of Cx40 and coronary microvascular dysfunction in type 2 diabetes
Authors: Si Rui , Cabrera Jody Tori O. , Tsuji-Hosokawa Atsumi , Gao Lei , Guo Rui , Watanabe Makiko , Lee Yun Sok , Moon Jae-su , Scott Brian T. , Wang Jian , Ashton Anthony W. , Rao Jaladanki N. , Yuan Jason X.-J. , Wang Jian-Ying , Makino Ayako 
Published: January 2021

Abstract
Background

Diabetic patients with coronary microvascular disease (CMD) exhibit higher cardiac mortality than patients without CMD. However, the molecular mechanism by which diabetes promotes CMD is poorly understood. RNA-binding protein HuR is a key regulator of mRNA stability and translation of many genes, and there is growing evidence showing the potential role of HuR in cardiovascular disease. In this study, we investigated the role of HuR and its target genes in the development of CMD in type 2 diabetic mice.
Methods

Type 2 diabetes was induced in male mice by a high-fat diet combined with a single injection of low-dose streptozotocin. We assessed coronary flow velocity reserve (CFVR, a determinant of coronary microvascular function) in vivo and isolated cardiac endothelial cells (CECs) from those mice for in vitro experiment. Coronary endothelial function was evaluated in the 3rd order of coronary arteries using a wire myograph. Human CECs from 4 control subjects and 4 diabetic patients were purchased from the company.
Results

Diabetic mice exhibited decreases in CFVR and capillary density in the left ventricle (LV). HuR protein levels in CECs were significantly lower in diabetic mice and diabetic patients than in the controls. Endothelial-specific HuR-KO mice also displayed significant reductions in CFVR and capillary density. By examining mRNA levels of 92 genes associated with endothelial function, we found that HuR, Cx40, and Nox4 levels were decreased in CECs from diabetic and HuR-KO mice in comparison to control mice. Cx40 protein level and HuR binding to Cx40 mRNA were downregulated in CECs from diabetic mice, and Cx40-KO mice exhibited decreased CFVR, attenuated endothelium-dependent relaxation, and reduced capillary density in the LV. Furthermore, endothelium-specific Cx40 overexpression ameliorated endothelial functions by augmenting endothelium-dependent relaxation and increasing capillary density in the LV, and resulted in the improvement of CFVR in diabetic mice.
Conclusions

These data suggest that decreased HuR, a specific mRNA binding protein that downregulates gap junction protein Cx40 in CECs, plays an important role in the development of coronary microvascular disease in diabetes. Restoration of Cx40 expression and function is potentially a novel therapeutic strategy for diabetic cardiovascular complications.
The Effectiveness of Enhanced Primary Healthcare (EnPHC) Interventions on Type 2 Diabetes Management in Malaysia: Difference-in-differences (DID) Analysis
Authors: husin masliyana , Teh Xin Rou , Ong Su Miin , Lim Yvonne Mei Fong , Ang Swee Hung , Chan Chee Lee , Lim Ming Tsuey , Shanmugam Sunita , Khamis Noraziani , Jaafar Faeiz Syezri Adzmin , Ibrahim Nor Idawaty , Nasir Nazrila Hairizan , Kusuma Dian , Wagner Anita Katharina , Ross-Degnan Dennis , Atun Rifat , Sivasampu Sheamini 
Published: January 2021

Abstract
Background

To evaluate the effectiveness of the Enhanced Primary Healthcare (EnPHC) interventions on process of care and intermediate clinical outcomes among type 2 diabetes patients.

Research Design and Methods

This was a quasi-experimental controlled study of multi-pronged interventions conducted in 20 intervention and 20 control public primary care clinics in Malaysia from November 2016 to June 2019. Malaysian patients aged 30 years and above with a diagnosis of type 2 diabetes were selected via systematic random sampling. We conducted difference-in-differences analyses of data on process of care and intermediate clinical outcomes extracted from medical records.

Results

We reviewed 12,017 medical records of patients with type 2 diabetes. Process of care measures improved: HbA1c tests performed within the past three months (odds ratio (OR) 3.31, 95% CI 2.13, 5.13); lipid test (OR 4.59, 95% CI 2.64, 7.97), LDL (OR 4.33, 95% CI 2.16, 8.70), and urine albumin (OR 1.99, 95% CI 1.12, 3.55) tests within the past year; BMI measured within the past six months (OR 15.80, 95% CI 4.78, 52.24); cardiovascular risk assessment (OR 174.65, 95% CI 16.84, 1810.80); and exercise counselling (OR 1.18, 95% CI 1.04, 1.33). We found no statistically significant changes in intermediate clinical outcomes.

Conclusions

EnPHC interventions were effective in improving process of care but not intermediate clinical outcomes for type 2 diabetes patients. The intervention package was successful in enhancing the quality of care in diabetes from the health provider perspective. Patient engagement and self-management support may be needed to bring forth changes in patient outcomes.
Risk Factors for Bacterial Infections in Patients With Moderate to Severe COVID-19: a Case Control Study
Authors: Nasir Nosheen , Rehman Fazal ur , Omair Syed Furrukh 
Published: January 2021

Abstract
ObjectiveBacterial infections are known to complicate respiratory viral infections and are associated with adverse outcomes in COVID-19 patients. A case control study was conducted to determine risk factors for bacterial infections where cases were defined as moderate to severe/critical COVID-19 patients with bacterial infection and those without were included as controls. Logistic regression analysis was performed. ResultsOut of a total of 50 cases and 50 controls, greater proportion of cases had severe or critical disease at presentation as compared to control i.e 80% vs 30% (p<0.001). Hospital acquired pneumonia (72%) and Gram negative organisms (82%) were predominant. Overall antibiotic utilization was 82% and was 64% in patients who had no evidence of bacterial infection. The median length of stay was significantly longer among cases compared to controls (12.5 versus 7.5 days) (p=0.001). The overall mortality was 30%, with comparatively higher proportion of deaths among cases (42% versus 18%) (p=0.009). Severe or critical COVID-19 at presentation (AOR: 4.42 times; 95% CI; 1.63-11.9) and use of steroids (AOR: 4.60; 95% CI 1.24-17.05) were independently associated with risk of bacterial infections. These findings have implications for antibiotic stewardship as antibiotics can be reserved for those at higher risk for bacterial superinfections.
Fairness in Household Financial Contribution to the Healthcare System; the Evidence of Iran from 2008 to 2018
Authors: Darvishi Ali , Amini-Rarani Mostafa , Mehrolhassani Mohammad Hossein , Yazdi-Feyzabadi Vahid 
Published: January 2021

Abstract
Objective: The achievement of equity in health leads to health gains and financial risk protection and this issue was one of the important goals of Iran’s health transformation programm (HTP). The present study aimed to how the HTP achieved its objectives in terms of fair financial protection by assessing the Fair Financial Contribution Index (FFCI) in various households of urban and rural areas before (2008-2013) and after (2014-2018) the implementation of the HTP. Results: According to the sample analyzed, 207,980 and 212,249 of households lived in urban and rural areas, respectively. The worst fair contributions to health expenditure in urban (FFCI= 0.684) and rural areas (FFCI= 0.530) were occurred in 2010 and 2009, respectively. Otherwise, the best fair contributions for urban areas (FFCI=0.858) and rural areas (FFCI= 0.836) were made in 2011. Before the HTP implementation was began (between 2008 and 2013), FFCI witnessed minor changes from 0.834 in 2008 to 0.833 in 2013. Following the HTP implementation, the FFCI values in urban and rural population declined (worsened) from 0.842 to 0.836 and 0.816 to 0.809, respectively. Overall, FFCI has been improved during years after the implementation of HTP. Such that, the improvement was shown more in rural areas.
The Effect of Stocking Density on the Slaughter Performance and Meat Quality of Pekin Ducks
Authors: Eratalar Sabri Arda , OKUR Nezih , Yaman Ahmet 
Published: January 2021

Abstract
BackgroundThe effect of stocking density on slaughter performance and meat quality were primarily investigated in this research. Pekin ducks were reared until slaughter age (42 days) in three different stocking density groups (three, five and seven ducklings/m2) in four replicate pens. To compare the slaughter performance of the ducklings’ live weight, carcass weight, carcass yield, thigh and breast meat weight and yield, and edible giblets weight (heart, liver and gizzard) were investigated. The meat quality was compared between the treatment groups based on dry matter ratio, cooking loss, water holding capacity, pH values, and colour parameters (L*, a*, b*, c*, h* and ΔE* values). Results Carcass weight, carcass yield, thigh and breast meat weight were found to decrease in parallel to the increasing stocking density, resulting in a reduction in thigh and breast meat weights and ratios (P˂0.05). Increasing the stocking density decreased the heart weight, and positively improved the liver and gizzard ratio (P˂0.05). However, it did not affect the meat quality parameters investigated in this research (P˂0.05). The breast meat of the ducks reared under higher stocking density had higher L*, h* and ΔE* values, lower a* values (P˂0.05), and similar b* and c* values (P˃0.05). ConclusionsEvaluating the overall research findings, it can be concluded that increased stocking density when rearing ducks negatively affects the slaughter performance where affecting only breast meat colour and weight of thigh meat with skin in investigated meat quality parameters. More detailed researches should be undertaken considering welfare and economic issues as well.
Cohort Profile: The China Northwest Cohort study
Authors: Li Chao , Liu Yezhou , Shi Guoshuai , Mi Baibing , Zeng Lingxia , Bi Yuxue , Li Qiang , Shen Yuan , Zhao Yaling , Pei Leilei , Kang Yijun , Xiao Shengbing , Liu Xin , Dai Jianghong , Zhang Yuhong , Wang Xinhua , Wang Xiaojie , Wang Duolao , Yan Hong , Dang Shaonong 
Published: January 2021

Abstract
BackgroundThe China Northwest Cohort study (CNC), a community population-based prospective observational study, aimed to investigate specific aetiology causes of NCDs and the long-term health hazards of NCDs in the northwest region of China with different ethnic groups.Study design and methodsA total of 118,572 participants (aged 35–74 years and including the Han, Uygur, Kazakh, Hui and Tibetan ethnic groups from five provinces of northwest China) were recruited between June 2018 and May 2019. Approximately 10% of the participants will be actively followed every 3 years via face-to-face interviews with the use of concise questionnaires to review risk exposures and disease incidence, as well as to review biological specimens, including blood, saliva and stool samples, which will be collected. Passive follow-ups will be conducted by periodic linking (every 6 months) of baseline survey data to establish electronic disease registries. The questionnaire survey, a regular medical examination and the storage of blood samples were conducted in the CNC baseline survey for all of the participants. Several other items from the medical examination were recorded for approximately 40% of the participants.DiscussionAmong the total participants, the proportions of Han, Uygur, Kazakh, Hui and Tibetan ethnicities were 75.3%, 13.0%, 1.7%, 8.2% and 1.3%, respectively. Many lifestyle and medical history factors were different across the ethnic groups. The genetic information from the multi-ethnic individuals, combined with abundant personal and environmental information, provided an important opportunity to reveal complex and specific mechanisms of the genetic and environmental factors associated with NCDs.
High Level of Mir-142-3P Expression in Peripheral Blood of Patients with Ovarian Carcinoma
Authors: Gider Yasemin , Jabbarli Xhariga , Uyaroglu Gamze , Tuncer Seref Bugra , Odemis Demet Akdeniz , Kurt Busra , Kilic Seda , Erdogan Ozge Sukruoglu , Celik Betul , Saip Pinar , YAZICI HÜLYA 
Published: January 2021

Abstract
Background
The most common cancers detected in women are breast, thyroid, colorectal, uterine corpus, lung, and ovarian cancer. Ovarian cancer is responsible from more than 150.000 death annually worldwide. This cancer is detected in the late stage, and is characterised with poor prognosis, therefore most cases result with death. The fact that this cancer manifests itself in the late stage and is characterized by a poor prognosis, is caused death in the majority of cases. Therefore, the diagnosis and the treatment of the disease have to be improved for a better quality of life for patients. MicroRNAs are the noncoding RNAs in the length of 19–24 nucleotides which show suppressor effect on target genes. miRNAs are included in the pathology of various diseases including cancer. miRNAs being as the biomarker candidates in diagnosis, and their use in treatment as the inhibitors of the molecules mimicking the miRNA showed that they may be used as the new therapeutic target and agents.
Methods
We detected with our group in our prior study conducted with disconcordant ovarian cancer twins that many miRNA molecules were different in ovarian cancer compared with the molecules in healthy sibling. The expression level of miR-142-3p that was selected from the miRNAs detected in the previous study was compared, and investigated in a wider ovarian cancer group, and in healthy control group. miR-142-3p expression level was investigated using the real-time PCR method in the present study involving 147 patients, and 100 healthy control group. The differences in the expression levels of miR-142-3p detected in the peripheral blood lymphocytes of ovarian cancer patients, and healthy control were statisticaly evaluated.
Results
The expression level of miR-142-3p was detected to have increased 3.11 fold in ovarian cancer patients compared with the levels in healthy controls, and the difference was statistically significant (p:0.00). These results suggest that miR-142-3p that was found significantly increased in the peripheral blood samples of ovarian cancer patients compared with the healthy controls might be used as a sensitive, noninvasive biomarker in the early diagnosis, and treatment and follow up of ovarian cancer.
Research on a&nbsp;ø6m TBM&nbsp;Steel Arch Splicing Manipulator
Authors: He Yuanfu , Xia Yimin , Xu Zhen , Yao Jie , Ning Bo , Xiao Xuemeng 
Published: January 2021

Abstract
As a challenging task, the robotic splicing of steel arch is required to realize the grasping and docking of steel arches in a limited space. The steel arches often have a mass of over 200kg and a length of over 4m. Due to the large volume and mass of steel arches and the high requirements for the positioning accuracy of splicing, it is difficult for the general manipulator to meet its flexibility and stiffness requirements. The single-degree-of-freedom(DOF) closed-loop mechanism has a simple and reliable structure. Adding it into the manipulator can effectively improve the dynamic performance and increase the structural stiffness. In this paper, a solution model of a single-DOF closed-loop planar mechanism is presented, and alternative kinematic pairs of the mechanism with different input constraints and output requirements are derived. Based on this model, a design method of steel arch splicing manipulator with single-DOF closed-loop grasping structure is proposed. All the optional basic configurations of the manipulator are deduced, and then the optimal configuration is obtained by using the performance indexes. A prototype of the steel arch splicing manipulator is manufactured, and the reliability of the manipulator is proved by experiments.