bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cisplatin, environmental metals, and cardiovascular disease: an urgent need to understand underlying mechanisms
Authors: Clasen Suparna C. , Dinh Paul C. , Hou Lifang , Fung Chunkit , Sesso Howard D. , Travis Lois B. 
Published: October 2021

AbstractSignificantly increased risks of cardiovascular disease occur in testicular cancer survivors given cisplatin-based chemotherapy. The postulated mechanism of platinum-based chemotherapy’s vascular toxicity has been thought secondary to its different early- and late- effects on vascular injury, endothelial dysfunction, and induction of a hypercoagulable state. We highlight for the first time the similarities between platinum-associated vascular adverse events and the vascular toxicity associated with other xenobiotic-metal contaminants. The vascular toxicity seen in large epidemiologic studies of testicular cancer survivors may in part be similar and mechanistically linked to the risk seen in environmental heavy metal contaminants linked to cardiovascular disease. Future research should be directed to better understand the magnitude of the adverse cardiovascular effects of platinum and to elucidate the underlying mechanisms of action.
RETRACTED ARTICLE: Impact of the third wave of the COVID-19 pandemic and interventions to contain the virus on society and patients with kidney disease in Cambodia
Authors: Nov Tam , Hyodo Toru , Kitajima Yukie , Kokubo Kenichi , Naganuma Toshihide , Wakai Haruki , Yamashita Akihiro , Phon Elin , Kawanishi Hideki 
Published: October 2021

AbstractCambodia detected its first case of COVID-19 just 3 days before WHO declared that the outbreak constituted as PHEIC. As of February 15, 2021, and after two major outbreaks, only 479 cases had been reported, 396 (83%) of which were imported. This small number of cases was largely thanks to stringent measures and policies put in place by the government to curb the spread. Despite these efforts, a third cluster outbreak was declared on February 20, 2021. It has disrupted all aspects of life in Cambodia. As in many other countries affected by the virus, economic hardship, lockdowns in cities, and food insecurity ensued. Against the backdrop of this widespread impact on the citizens of Cambodia, we conducted this review article to better understand the situation of healthcare workers in nephrology and dialysis patients and the challenge they face in providing and receiving essential medical care. Healthcare providers have continued working to serve their patients despite facing a high risk of catching SARS-CoV-2 and other challenges including difficulties in traveling to work, increased physical and mental burden, and higher stress due to measures taken to minimize the risk of transmission during patients’ care. Some healthcare workers have been discriminated against by neighbors. The most difficult mission is when having to deal with families whose loved one is denied access to a hemodialysis session due to suspected COVID-19 while waiting for PCR test results. Hemodialysis patients reported facing economic hardship and increasingly difficult circumstances. When access to food is limited, patients have eaten canned or dried salted food rather than an appropriate hemodialysis diet. Because hemodialysis centers are concentrated in a few cities, access has become even harder during the travel ban. In-center hemodialysis rules are stricter and does not allow family members or escorts to enter the unit. Only a few hemodialysis patients have been vaccinated. Before COVID-19, hemodialysis patients already faced major burdens. The pandemic appears to be decreasing their quality of life and survival even further. Through this study, we have revealed current hardships and the need to improve the situations for both healthcare workers in nephrology and hemodialysis patients in Cambodia.
The effect of transcranial direct current stimulation of dorsolateral prefrontal cortex on performing a sequential dual task: a randomized experimental study
Authors: Abedanzadeh Rasool , Alboghebish Saeed , Barati Parisa 
Published: October 2021

AbstractWhen it comes to simultaneous processing of two tasks, information processing capacity is usually below par and not desirable. Therefore, this preliminary study aimed to investigate the effect of transcranial direct-current stimulation (tDCS) of dorsolateral prefrontal cortex (DLPFC) on performing dual tasks. Twenty-six students (average age 25.2 ± 2.43 years) were selected and then randomly divided into experimental and sham groups. All of the participants conducted the Stroop effect test in a dual task situation before and after the tDCS. This test included two intervals between the stimuli of 100 and 900 ms. The results of mixed-ANOVA showed that the average second reaction time of the experimental stimulated group was reduced (in both dual tasks with congruent and incongruent stimuli) significantly after the tDCS. Therefore, it can be stated that the tDCS of the DLPFC increases the information processing speed and the capacity of attention and, as a result, decreases the effect of the psychological refractory period.
Measuring respiratory symptoms in moderate/severe asthma: evaluation of a respiratory symptom tool, the E-RS®: COPD in asthma populations
Authors: Tabberer Maggie , von Maltzahn Robyn , Bacci Elizabeth D. , Karn Hayley , Hsieh Ray , Howell Timothy A. , Bailes Zelie , Fowler Andrew , Lee Laurie , Murray Lindsey T. 
Published: October 2021

Abstract
Background
Symptom constructs included in the Evaluating Respiratory Symptoms in Chronic Obstructive Pulmonary Disease (E-RS®: COPD) tool may be relevant to patients with asthma. The purpose of this study was to evaluate content validity and psychometric performance of the E-RS: COPD in moderate/severe asthma patients.

Methods
Content validity of the E-RS: COPD was evaluated in patients with moderate/severe asthma using concept elicitation and cognitive debriefing interviews. Secondary analyses using data from two clinical trials in patients with moderate/severe asthma evaluated the factor structure of the E-RS: COPD plus two supplementary items (wheeze; shortness of breath with strenuous physical activity) and assessed psychometric properties of the tool, which will be referred to as E-RS®: Asthma when used in asthma populations.

Results
Qualitative interviews (N = 25) achieved concept saturation for asthma respiratory symptoms. Concepts in the E-RS: COPD were relevant to patients and instructions were understood. Most patients (19/25; 76%) reported experiencing all concepts in the E-RS: COPD; no patients indicated missing symptoms. Secondary analyses of clinical trial data supported the original factor structure (RS-Total and three symptom-specific subscales). The two supplemental items did not fit with this factor structure and were not retained. RS-Total and subscale score reliability was high (internal consistency [α] > 0.70). Validity was demonstrated through significant (P < 0.0001) relationships with the St George’s Respiratory Questionnaire (SGRQ) and Asthma Symptom Severity scale. E-RS: Asthma was responsive to change when evaluated using SGRQ, Patient Global Impression of Change and Asthma Quality of Life Questionnaire as anchors (P < 0.0001). Clinically meaningful change thresholds were also identified (RS-Total: − 2.0 units).

Conclusions
The E-RS: Asthma is reliable and responsive for evaluating respiratory symptoms in patients with moderate/severe asthma.
Exposure of Exiguobacterium spp. to dengue vector, Aedes aegypti reduces growth and reproductive fitness
Authors: Rajagopal Gopalan , Ilango Sakkanan 
Published: October 2021

AbstractBackgroundAedes aegyptiis a major insect vector because it transmits dreadful viruses as adults that cause disease in humans and other vertebrates. The use of mosquito’s microbiota has shown great potential impacts on vector control and mosquito reproductive competence. The present study aimed to examine the resident bacteria of mosquitoes which are used as a potent range to reduce theA. aegyptifitness. Isolated resident-bacterial strains from blood-fedAedesspecies were characterized using gene sequencing and phylogenetic analysis, to assess the inhabitant bacterial strains survival rate inA. aegyptimidgut, instar developmental duration, malformation and reproductive competence.ResultsThe genetic distinctiveness of isolated bacterial strains belong to the genusExiguobacteriumspp. and further non-redundant nucleotide database search revealed that the species of effective strains wereE. aestuarii(MN629357) andE. profundum(MN625885). Exposure of the freshly hatched larvae with these bacteria cell densities extended the developmental duration. For instance, exposure ofA. aegyptilarva with 0.42 × 108, 0.84 × 108and 1.68 × 108cells/mL ofE. aestuariiextended the total developmental duration to 11.41, 14.29 and 14.78 days, respectively. It also reduced the fecundity and hatchability ofA. aegyptifemale, with exposure to these bacteria, from 1033.33 eggs/10 females in the control series to 656.67 eggs/10 females.ConclusionsThese present findings indicate that the resident-bacterial strains from blood-fed mosquito not only extend the larval durations but also rendered theA. aegyptifemales sterile to various extents.
Screening of factors that influence the preparation of Dialium guineense pods active carbon for use in methylene blue adsorption: a full factorial experimental design
Authors: Jasper Enebi Estella , Onwuka Jude Chinedu , Bidam Yakubu Manasseh 
Published: October 2021

Abstract
Background
The use of active carbons derived from waste biomass as adsorbents in the remediation of wastewater remains a valuable and cost-effective technology when compared to the use of commercial active carbon for the same purpose. This research aims at using a 2-level full factorial design (FFD) to efficiently evaluate factors that influence the preparation of active carbon from the waste pods of the Dialium guineense seeds. The influence of three preparation factors (concentration of the activating agent, activation time, and type of activating agent) on the active carbon yield and its adsorption capacity for methylene blue were investigated. Based on the full factorial design, two regression models were developed to correlate the factors to the two responses. From an analysis of variance (ANOVA), the most significant factors influencing each response were identified. The active carbon preparation conditions were then optimized by maximizing both the active carbon yield and its adsorption capacity for Methylene Blue. The functional group and surface morphology of the active carbon prepared under the predicted optimum conditions was analyzed via Fourier Transform Infra-Red (FTIR) spectroscopy and Scanning Electron Microscopy (SEM) respectively.

Results
The results of this study revealed that the concentration of the activating agent had the most significant effect on the yield of the active carbon produced as well as on its adsorption capacity for methylene blue. The optimum preparation process conditions were found to be: concentration of activating agent 5M, activation time 30 min and activating agent, NaOH which resulted in an active carbon yield of 21.25%, and an adsorption capacity of 9.33509 mg MB per gram of active carbon. SEM and FTIR showed evidence of successful activation.

Conclusion
The preparation of Dialium guineense seed pods' active carbon is strongly influenced by concentration and type of activating agent used. Reliable statistical models based on the FFD proved to be useful in identifying factors that significantly influence the preparation of Dialium guineense seed pods' active carbon. The active carbon holds great potential for application in the elimination of hazardous synthetic dyes from wastewater and should be explored further.
Translation and cross-cultural adaptation of “Trapeziometacarpal Arthrosis Symptoms and Disability—TASD” into Brazilian Portuguese
Authors: de Souza Almeida Vinícius Alexandre , Fernandes Carlos Henrique , Meireles Lia Miyamoto , Faloppa Flavio , Ejnisman Benno , Cohen Moises 
Published: October 2021

Abstract
Background
Osteoarthritis is the most common form of hand arthritis and arthritis of the carpometacarpal joint of the thumb is a potentially limiting disease. There is no homogeneity in the evaluation of outcomes for the rhizarthrosis treatment. In an attempt to standardize the evaluation of results, some subjective questionnaires, non-specific, were used to evaluate rhizarthrosis. Trapeziometacarpal Arthrosis Symptoms and Disability (TASD) was described by Becker et al.with the purpose of evaluating symptom intensity and degree of disability, as to compare results after treatment. Our objective is to translate, validate and do the cultural adaptation of the questionnaire TASD into the Brazilian Portuguese.

Methods
The questionnaire was translated, with reverse translation. The translations were evaluated and synthesized by a committee, arriving at TASD-BR. Thirty-one patients with a diagnosis of rhizarthrosis answered the questionnaire. We evaluated, the internal consistency, reliability, agreement and ceiling and floor effect for validation.

Results
The questionnaires were translated and adapted according to defined protocols. The internal consistency, through Cronbach's α coefficient for TASD-BR, was 0.927. The questionnaire's reliability, through the Intraclass Correlation Coefficient, was also shown to be quite high, with κ = 0.961 (0.954–0.967). The agreement, measured through the Standard Error Measurement, remained with standardized values below 5%. There was no ceiling and floor effect.

Conclusion
Through specific methodology we consider TASD-BR translated and valid for the Brazilian Portuguese.
High throughput in vitro characterization of pectins for pig(let) nutrition
Authors: Wiese Maria , Hui Yan , Holck Jesper , Sejberg Jimmy J. P. , Daures Celia , Maas Evy , Kot Witold , Borné Johanna M. , Khakimov Bekzod , Thymann Thomas , Nielsen Dennis Sandris 
Published: October 2021

Abstract
Background
Fiber-rich feed components possess prebiotic potential to enhance pig health and are considered a potential solution to the high prevalence of post-weaning diarrhea in pig production under the phased suspension of antibiotics and zinc oxide use.

Methods
We screened the gut microbiota modulatory properties of pectin substrates prepared from sugar beet within the freshly weaned piglet gut microbiome using an in vitro colon model, the CoMiniGut. We focused on testing a variety (13) of sugar beet-derived pectin substrates with defined structures, as well as known prebiotics such as inulin, fructooligosaccharide (FOS) and galactooligosaccharide (GOS), to gain insights on the structure–function related properties of specific substrates on the weaner gut microbial composition as well as shortchain fatty acid production (SCFA).

Results
Sugar beet-derived pectin and rhamnogalacturonan-I selectively increased the relative abundance of Bacteroidetes, specifically Prevotella copri, Bacteroides ovatus, Bacteroides acidificiens, and an unclassified Bacteroides member. The degree of esterification impacted the relative abundance of these species and the SCFA production during the in vitro fermentations. Modified arabinans derived from sugar beet promoted the growth of Blautia, P. copri, Lachnospiraceae members and Limosilactobacillus mucosae and amongst all oligosaccharides tested yielded the highest amount of total SCFA produced after 24 h of fermentation. Sugar beet-derived substrates yielded higher total SCFA concentrations (especially acetic and propionic acid) relative to the known prebiotics inulin, FOS and GOS.

Conclusion
Our results indicate that the molecular structures of pectin, that can be prepared form just one plant source (sugar beet) can selectively stimulate different GM members, highlighting the potential of utilizing pectin substrates as targeted GM modulatory ingredients.
Microbiome study in irradiated mice treated with BIO 300, a promising radiation countermeasure
Authors: Cheema Amrita K. , Li Yaoxiang , Singh Jatinder , Johnson Ryan , Girgis Michael , Wise Stephen Y. , Fatanmi Oluseyi O. , Kaytor Michael D. , Singh Vijay K. 
Published: October 2021

Abstract
Background
The mammalian gut harbors very complex and diverse microbiota that play an important role in intestinal homeostasis and host health. Exposure to radiation results in dysbiosis of the gut microbiota leading to detrimental pathophysiological changes to the host. To alleviate the effects of irradiation, several candidate countermeasures are under investigation. BIO 300, containing synthetic genistein formulated as an amorphous solid dispersion or as an aqueous suspension of nanoparticles, is a promising candidate under advanced development. The aim of this study was to investigate the effects of BIO 300 on the gut microbiome and metabolome of mice exposed to 60Co gamma-radiation. The gut microbiota and metabolome of control and drug-treated mice exposed to radiation was characterized by bacterial 16S rRNA amplicon sequencing and untargeted metabolomics.

Results
We found that irradiation altered the Firmicutes/Bacteroidetes ratio and significantly decreased the relative abundance of Lactobacillus, both in BIO 300-treated and control mice; however, the ratio returned to near normal levels in BIO 300-treated mice by day 14 post-irradiation. Concomitantly, we also observed corrective shifts in metabolic pathways that were perturbed after irradiation.

Conclusions
Overall, the data presented show that radiation exposure led to a relative depletion of commensals like Lactobacillus leading to an inflammatory metabolic phenotype while the majority of the drug-treated mice showed alleviation of this condition primarily by restoration of normal gut microbiota. These results indicate that the radioprotective effects of BIO 300, at least in part, may involve correction of the host-microbiome metabolic axis.
Traditional food preparation of wild edible vegetables among the ethnic groups of Mizoram, North East India
Authors: Lalmuanpuii Rosie , Zodinpuii Betsy , Lalbiaknunga J. , Zothanpuia  
Published: October 2021

AbstractExploration of ethnic traditional food recipes has an immense contribution to the preservation and sustainability of traditional food habits and culture. The main objective of this study was to explore and document traditional food recipes prepared from wild edible vegetables among two ethnic groups of Mizoram, Northeast India. The method employed for this study was mainly based on a household survey; randomly selected 35 households in Aizawl district representing the major ethnic groups, namely Hmar and Paihte. Information was collected on different types of wild edible vegetables ethnic food, as prepared and consumed by the local people. Twenty-four types of traditional food recipes were documented according to their seasonal availability, and the affinities of choices in food consumption are comparable among the two ethnic groups. It was also reported that wild edible vegetables play a significant role in safeguarding food security and improve nutrition in diets. The present work documents the wild edible vegetables used traditionally by two ethnic groups of Mizoram for various recipes and highlights the necessity of conserving such traditional knowledge besides throwing light on their economic potential. The study suggests that future investigation be carried out to evaluate the nutritional and bioactive properties of wild edible vegetables.
Fidelity of kangaroo mother care services in the public health facilities in Bangladesh: a cross-sectional mixed-method study
Authors: Mehjabeen Saima , Matin Mowtushi , Gupta Rajat Das , Sutradhar Ipsita , Mazumder Yameen , Kim Minjoon , Sharmin Shamina , Islam Jahurul , Sarker Malabika 
Published: October 2021

Abstract
Background
Kangaroo mother care (KMC) is a proven low-cost intervention to prevent neonatal mortality of pre-term and low birth weight babies and is very relevant to Bangladesh. KMC provides thermal regulation and thus directly avert neonatal mortality. KMC includes early, continuous, and prolonged skin-to-skin contact between an infant and caregiver, exclusive breastfeeding, early discharge from the hospital, and post-discharge follow-up. The purpose of this study was to investigate the fidelity of this intervention’s implementation according to national guidelines across all tiers of government (public) health facilities of Bangladesh.

Methods
We adopted a triangulation mixed-methods approach of both quantitative and qualitative components in this research to support and explain the information obtained from quantitative observation with the help of qualitative interviews on the fidelity of KMC practice. We used an observation checklist to find the fidelity of KMC practice and used semi-structured guidelines to explain and understand the moderators of fidelity through key informant interviews and in-depth interviews. We undertook eight facility visits in four districts, observed twenty-three neonates and their caregivers during KMC practice at those facilities, and conducted twenty-seven key informant interviews with facility managers, health care providers, and five in-depth interviews with caregivers. Extracted information was triangulated and arranged under the themes of the fidelity framework.

Results
Despite being a low-cost intervention, findings exhibit some adherence to the national guideline with several gaps in practice. Leadership played a critical role in ensuring the KMC practice. Specific components of KMC practice, like duration, nutrition maintenance, discharge criteria, and follow-up, were not consistent as recommended. Infrastructure, human resources, developmental partner support, and the demand-side and supply-side responsiveness played a critical role in enacting this human-centric approach’s fidelity. The observed interruption found in the implementation process posed threats to achieve the intended outcome as these caused violations of the basic principles of KMC.

Conclusions
The study findings will help find ways to effectively deliver this intervention so that fidelity of practice is maintained, enhancing KMC services’ quality and advocating towards the successful scale-up of this program.
In silico screening and molecular docking study of compounds from Pedalium murex L. with Vasopressin2 receptor target for Autosomal Dominant Polycystic Kidney Disease
Authors: Ram Gobind , Kumar Anil , Hemlata  , Singh Gulab , Giri Shiv Kumar 
Published: October 2021

Abstract
Background
Autosomal dominant polycystic kidney disease (ADPKD) is frequently inherited disease. The medicinal plant Pedalium murex (P. murex) Linn, that has anti-inflammatory, antiurolithiatic, and diuretic properties, has a greater tendency to cure urinary defects. P. Murex compounds have been studied in order to find an effective treatment against the Vasopressin 2 receptor (V2R), which is a target for ADPKD. The compound structures were designed using ChemSketch software, which was then optimised for the exploration of pharmacokinetic properties. Finally, AutoDock VINA programme was used to execute molecular docking, and the findings were analysed and visualised in Discovery studio visualizer.

Results
Virtual screening using PyRx software finds seven compounds from P. murex with binding affinities ranging from − 8.6 to − 5.8 kcal/mol, which will be used for further pharmacological characteristics study. Luteolin has a higher druglikeness and an overall drug score of 0.84, indicating as a most suitable compound. Furthermore, luteolin docking and bonding study reveals improved receptor (V2R) H-bonding with Phe105(2.26 and 2.96), Gln119(2.78), and any Lys116(2.16).

Conclusions
Based on affinity score, screening of various compounds from P. murex against the V2R target for the ADPKD showed that the phytocompound luteolin has superior pharmacological characteristics and bonding. Luteolin from P. murex can be used as a possible therapeutic candidate after rigorous in silico investigation.

Graphic abstract