bluuebunny/crossref_metadata_2025_split huggingface dataset:
Serum Lactate and Acute Mesenteric Ischemia: An Observational, Controlled Multicenter Study
Authors: Collange Olivier , LOPEZ Marc , LEJAY Anne , PESSAUX Patrick , OUATTARA Alexandre , DEWITTE Antoine , RIMMELE Thomas , GIRARDOT Thibaut , ARNAUDOVSKI Darko , AUGUSTIN Pascal , CHAKFE Nabil , TACQUARD Charles , OULEHRI Walid , ZIELESKIEWICZ Laurent , SEVERAC François , LEONE Marc , MERTES Paul Michel 
Published: June 2021

Abstract
Purpose

Early diagnosis of acute mesenteric ischemia (AMI) is key to survival but remains extremely difficult, as the symptoms are vague and non-specific. Although international guidelines recommend that serum lactate is not used for AMI diagnosis, this parameter is still specifically taken into account for the diagnosis and prognosis of AMI. Our hypothesis was that serum lactate levels cannot be used to diagnose AMI or predict its outcome.
Methods

This was an ancillary, retrospective, observational, controlled multicenter study, approved by an Ethics Committee. Patients with AMI at adult intensive care unit (ICU) admission were included (AMI group) and matched to ICU patients without AMI (control group). Matching criteria were center, period, gender, age, and severity. Serum lactate levels were measured on day 0 (D0) and day 1 (D1) and the lactate difference (D0-D1) was calculated.
Results

Two hundred and seventy-four patients were included. Serum lactate levels were elevated in both groups at D0 and D1 but there was no significant difference between groups at D0 (2.7 [1.3; 5.5] vs 3.4 [1.9; 6.1] mmol/L; p = 0.284), D1 (1.8 [1.2; 3.1] vs 2.4 [1.5; 4.6] mmol/L, p = 0.547; respectively for control and AMI groups) or D0-D1. Thus, the performance of serum lactate for AMI diagnosis was poor. Concerning AMI outcome, serum lactate levels predicted ICU death in both groups at D0 and D1.
Conclusions

no specific association was observed between serum lactate levels and AMI. Serum lactate should not be used for the diagnosis of AMI but may help assess disease severity.
Assessment of Land Use And Land Cover Change Detection And Prediction Using Remote Sensing And CA Markov In The Northern Coastal Districts of Tamil Nadu, India
Authors: abijith Devanantham , Saravanan Subbarayan 
Published: June 2021

Abstract
Land use and land cover (LULC) change analysis and forecasting aids the upcoming generation in research and evaluate the global climate change for managing and controlling environmental sustainability. This research analyzes the Northern TN coast, which is under both natural and anthropogenic stress. The analysis of LULC changes and LULC projections for the region between 2009-2019 and 2019-2030 was performed utilizing Google Earth Engine (GEE), TerrSet, and Geographical Information System (GIS) tools. LULC image is generated from Landsat images and classified in GEE using Random Forest (RF). LULC maps were then framed with the CA- Markov model to forecast future LULC change. The CA-Markov’s Land change modeler (LCM) was set up to create future LULC. It was carried out in four steps: (1) Change analysis, (2) Transition potential, (3) Change prediction, and (4) Model validation. For analyzing change statistics, the study region is divided into zone 1 and zone 2. In both zones, the water body shows a decreasing trend, and built-up areas are in increasing trend. Barren land and vegetation classes are under stress and developing into built-up. The overall accuracy was above 89%, and the kappa coefficient was above 87% for all three years. This region is highly susceptible to inland floods, coastal floods, and other natural disasters; thus, this study’s results support future development plans and decision-making.
Analysis of Morphological and Molecular Genetic Diversity of Salicornia Iranica Akhani Wildlife Populations around Urmia Lake, Iran
Authors: Aghaei Mohammad , Hassani Abbas , Nazemiyeh Hossein , Mandoulakani Babak Abdollahi , Saadatian Mohammad 
Published: June 2021

Abstract
Salicornia is a halophyte plant capable of being irrigated with seawater, which can be used as an alternative food. Given this, it is necessary to study the potentials of this plant's morphological diversity in the natural environment. In this study, 33 wild populations of Salicornia were collected from different geographical areas around Urmia Lake during the flowering stage, and some morphological traits and 25 ISSR loci of the plant were measured. Based on morphological traits and the cluster analysis, Salicornia populations were divided into four groups. Overall, the high percentage of polymorphic gene loci (65.69%), the average number of effective alleles per gene locus (1.63), and the Shannon data index (0.540) indicate that ISSR markers can be used in Identify genetic diversity to be used. Molecular data cluster analysis divided the studied populations into two main groups, which included 12.12% and 87.88% of the populations, respectively. Based on the effective analysis of the population's genetic structure and the precise classification of individuals into suitable sub-populations, the value of K = 2 was calculated. The research findings indicated that markers UBC823, B, A7, and K, and with the Shannon index, effective allele, and large heterozygosity values are markers with the highest effectiveness compared to other markers utilized, and they are used better than other compounds in genetic distance. The findings of this study will aid in parental selection studies for breeding programs of salicornia in future.
The Face of Hypervirulent Klebsiella Pneumoniae (hvKp) Isolated from Clinical Samples of Two Iranian Teaching Hospitals
Authors: Sanikhani Rahimeh , Moeinirad Mohammad , Solgi Hamid , Haddadi Azar , Shahcheraghi Fereshteh , Badmasti Farzad 
Published: June 2021

Abstract
Hypervirulent Klebsiella pneumoniae (hvKp) has emerged as a pathogen of global concern. In this study, both phenotypic and genotypic tests were used to detect hvKp. Antimicrobial resistance profiles and clonal relatedness of clinical isolates were also determined. We found that 62.6% of the isolates were tellurite resistant and among them iucA or iutA or peg344 as hvKp molecular markers, were positive. The blaSHV (81.4%), followed by blaCTX−M15 (75.5%) and blaTEM (67.6%), blaOXA−48 (33.7%), blaNDM−1 (32.3%) were detected, while blaKPC−1 was not present in any hvKp isolates. It was found that the majority of hvKp isolates belonged to capsular serotype K20 and ompK36 group C, which is related to CG23 (e.g. ST23). A high percentage of multidrug-resistant hvKp (MDR-hvKp) and high resistance to imipenem (66%) indicated that there is an urgent problem that should be addressed in the clinical settings.
Molecular Mechanisms of Danhong Injection in Treatment of Pulmonary Fibrosis Based on Network Pharmacology and Experimental Validation
Authors: Han Di , Xu Yong , Peng Wenpan , Wang Dan , Huang Tongxing , Zhou Xianmei , Shan Jinjun 
Published: June 2021

Abstract
Backgroud: Danhong injection has been proven to be reliable and practical in the clinical treatment of Pulmonary Fibrosis (PF), whereas the mechanism of action is unclear. The aim of this study was to investigate the mechanism of action of Danhong injection in the treatment of PF through network pharmacology and experimental validation. Methods: In this paper, multiple databases were utilized to capture the targets of Danhong injection and PF disease-related targets. The interaction network such as active ingredient-target was constructed and protein interaction analysis was performed via Cytoscape software. Bioprocess and signaling pathway analysis of the targets was carried out through DIVID database. Based on the analytical results, pharmacodynamic validation and mechanistic investigation were conducted in mice. Results: In this study, 111 compounds and common targets for diseases were filtered, with key targets involving MDM2, IL-2, CCL5, AKT1, MMP9, CASP3, MMP2, etc. The KEGG and GO enrichment analysis identified 16 significantly different signaling pathways, encompassing bioprocesses such as cell proliferation, apoptosis, programmed death and immune response. Animal experiments demonstrated that Danhong injection could reduce bleomycin-induced PF in mice, and could decrease the mRNA of AKT1 and the protein expression of p-Pi3k and p-Akt, thereby reducing apoptosis in mouse lung tissues. Conclusions: This study initially revealed that Danhong injection may inhibit apoptosis through the Pi3k/Akt signaling pathway and thus reduce PF in mice.
The Olsenella Uli Induced Pneumonia: A Case Report
Authors: Yan Yufen , Li Hong , Li Shuai , Liu Shuhui , Jia Nan , Liu Yanfei , Liu Qing , Han Chunhua 
Published: June 2021

Abstract
Olsenella uli is a Gram-positive bacterium common in the oral cavity or gastrointestinal tract. Here we reported a first case of human pneumonia caused by the Olsenella uli. The identification of Olsenella uli was based on micromorphology, sequence analysis and mass spectrometry analysis of the bacteria recovered from sputum. Ceftazidime，one of the third generation cephalosporins was used for the anti-infection treatment of the patient. CT results showed a significant improvement of the pulmonary lesion and pleural effusion and recovery from pulmonary infection after 10 days. The mechanism underlying Olsenella uli induced pneumonia is unclear, our report suggests a causative role of gingival bacteria in pathogenesis of pneumonia, and the intervention by Ceftazidime may offer a therapeutic strategy for Olsenella uli infection.
Documenting Response To COVID-Individual and Systems Successes and Challenges: A Longitudinal Qualitative Study
Authors: Shaukat Natasha , Ali Daniyal Mansoor , Hisam Butool , Hassan Sheza , Barolia Rubina , Afzal Badar , Khan Abdus Salam , Angez Meher , Razzak Junaid 
Published: June 2021

Abstract
Background

This feasibility study aimed to assess the use of WhatsApp for qualitative data collection to document the evolution of perceptions of frontline healthcare workers (FHCW) regarding their wellbeing and the quality of health systems' response to the COVID-19 pandemic over four months.
Methods

This was a prospective longitudinal qualitative study conducted during the four months coinciding with the peak and trough of the first wave of the COVID-19 pandemic (June-September 2020). We approached frontline healthcare workers (physicians and nurses working in emergency departments) in two hospitals using the WhatsApp group of the Pakistan Society of Emergency Physicians (PSEM). We introduced the study and obtained consent using a google consent form. Each participant was asked to self-record their perception of their personal wellness and their level of satisfaction with the quality of their hospitals' response to the pandemic. Each participant sent their voice notes/audio-recording to a central WhatsApp number. We transcribed and analysed the recordings and identified themes and sub-themes, and the changes to these themes over six months.
Results

We invited approximately 200 FHCWs associated with PSEM to participate in the study. Of the 61 who agreed to participate, 27 completed the study. A total of 149 audio recordings were received and transcribed. Three themes and eight sub-themes have emerged from the data. The themes were individual-level challenges, health system-level challenges, and hope for the future. Sub-themes for individual-level challenges were: fear of getting or transmitting infection, financial stressors, stress due to turning away those patients who need care, anxiety due to the general public's lack of compliance with preventive measures, physical exhaustion, and fatigue. For the healthcare system, sub-themes were: issues with logistics and management of the hospital/healthcare system and lack of focus on providing air conditioning to address heat due to PPEs and sub-themes under hope for the future were the improved disease knowledge and vaccine development.
Conclusion

Despite a lower level of completion, our study identified possible use of a ubiquitously available mobile app to collect longitudinal real-time data from FHCWs during the initial period of the pandemic. The overall perceptions and experiences of FHCWs evolved from negative to positive as the curve of COVID-19 went down.
Clinical presentation and CT features in pediatric patients with COVID‐19 infection
Authors: Solgun Huseyin Avni , Yurdaısık Isıl 
Published: June 2021

Abstract
Background

The aim of this study includes to discuss the clinical, laboratory, and chest computed tomography (CT) in pediatric patients with 2019 novel coronavirus (COVID-19) infection.
Material and Methods

The clinical, laboratory, and chest CT features of 17 pediatric inpatients with COVID-19 infection confirmed by pharyngeal swab COVID‐19 polymerase chain reaction(PCR). All clinical and laboratory data have been recorded and analyzed during march-february 2021. Chest CT have been performed to all Covid 19 PCR confirmed patients and radiologicall view have been noted.
Results

Seventeen pediatric patients with a history of close contact with COVID-19 diagnosed family members included to the study. Fever (10/17, 58%) and cough (13/17, 76%) were the most common symptoms. For laboratory findings, c reactive protein elevation (15/17, 88%) seem to be the most finding. A total of 4 patients presented with unilateral pulmonary lesions (4/17, 23%), 9 with bilateral pulmonary lesions (9/17, 52%) and 13 cases showed bilateral diffuse covid pattern on chest CT (13/17, 76%). Non-spesific consolidation with was observed in 8 patients (8/17, 47%), ground‐glass opacities were observed in 11 patients (11/17, 64%), nodules were observed in 7 patients (7/17, 41%), and tiny nodules were observed in 2 patients (2/17, 11%).
Conclusion

In pediatric patients with positive COVID-19 nucleic acid test from pharyngeal swab samples; the early detection of lesions by CT can be efficient; in management and early treatment for pediatric patients. However; early chest CT screening and COVİD-19 PCR testing together can be more efficent in diagnose.
SMC4, CCNB1 and CKS1B as potential targets and new critical biomarkers for the prognosis of human bladder cancer
Authors: Fang Hongpeng , Huang Zhansen , Zeng Xianzi , Wan Jiaming , Wu Jieying , Di Jinming 
Published: June 2021

Abstract
Background
As a common malignant cancer of the urinary system, the precise molecular mechanisms of bladder cancer remain to be illuminated. The purpose of this study was to identify core genes with prognostic value as potential oncogenes for the diagnosis, prognosis or novel therapeutic targets of bladder cancer.
Methods
The gene expression profiles GSE3167 and GSE7476 were available from the Gene Expression Omnibus (GEO) database. Next, PPI network was built to filter the hub gene through the STRING database and Cytoscape software and GEPIA and Kaplan-Meier plotter were implemented. Frequency and type of hub genes and sub groups analysis were performed in cBioportal and ULCAN database. Finally,We used RT-qPCR to confirm our results.
Results
Totally, 251 DEGs were excavated from two datasets in our study. We only founded high expression of SMC4, TYMS, CCNB1, CKS1B, NUSAP1 and KPNA2 was associated with worse outcomes in bladder cancer patients and no matter from the type of mutation or at the transcriptional level of hub genes, the tumor showed a high form of expression. However, only the expression of SMC4,CCNB1and CKS1B remained changed between the cancer and the normal samples in our results of RT-qPCR.
Conclusion
In conclusion,These findings indicate that the SMC4,CCNB1 and CKS1B may serve as critical biomarkers in the development and poor prognosis.
Maximum Lesion-to-contralateral Normal Brain Tissue Ration of 11C-methionine PET as a Prognostic Imaging Biomarker for Newly Diagnosed and Untreated Astrocytic Glioma
Authors: Nakajo Kosuke , Uda Takehiro , Kawashima Toshiyuki , Terakawa Yuzo , Ishibashi Kenichi , Tsuyuguchi Naohiro , Tanoue Yuta , Nagahama Atsufumi , Koh Saya , Uda Hiroshi , Sasaki Tsuyoshi , Ohata Kenji , Kanemura Yonehiro , Goto Takeo 
Published: June 2021

Abstract
Purpose: This study aimed whether the uptake of amino tracer positron emission tomography (PET) can be used as an additional imaging biomarker to estimate the prognosis of glioma.Methods: Participants comprised 56 adult patients with newly diagnosed and untreated World Health Organization (WHO) gradeⅡ-Ⅳ astrocytic glioma who underwent surgical excision and were evaluated by 11C-methionine PET prior to the surgical excision at Osaka City University Hospital from July 2011 to March 2018. Clinical and imaging studies were retrospectively reviewed based on medical records at our institution.Results: Preoperative Karnofsky Performance Status (KPS) only influenced progression-free survival (PFS) (hazard ratio [HR] 0.20; 95% confidence interval [CI] 0.10-0.41, p<0.0001), whereas histology (anaplastic astrocytoma: HR 5.30, 95%CI 1.23-22.8, p=0.025; glioblastoma: HR 11.52, 95%CI 2.27-58.47, p=0.0032), preoperative KPS≥80 (HR 0.23, 95%CI 0.09-0.62, p=0.004), maximum lesion-to-contralateral normal brain tissue (LN max)≥4.03 (HR 0.24, 95%CI 0.08-0.71, p=0.01), and isocitrate dehydrogenase (IDH) status (HR 14.06, 95%CI 1.81-109.2, p=0.011) were factors influencing overall survival (OS) in multivariate Cox regression. OS was shorter in patients with LN max≥ 4.03 (29.3 months) than in patients with LN max<4.03 (not reached; p=0.03). OS differed significantly between patients with IDH mutant/LN max<4.03 and patients with IDH mutant/LN max ≥4.03.Conclusions: LN max using 11C-methionine PET may be used in prognostic markers for newly identified and untreated WHO gradeⅡ-Ⅳ astrocytic glioma.
Prevalence of Dengue Virus, Chikungunya and Zika Infection in Blood Donors of Northern of Brazil
Authors: Lamarão Leticia Martins , Correa Angelita Silva Miranda , de Castro Renata Bezerra Hermes , Amaral Carlos Eduardo de Melo , Monteiro Patricia Danin Jordão , Palmeira Mauricio Koury , Lopes Luane Nascimento , Oliveira Angela Neves , de Lima Maria Salete Maciel , Burbano Rommel Rodríguez 
Published: June 2021

Abstract
We identified the prevalence of infection by the Dengue virus, Chikungunya and Zika in blood donors in epidemic and non-epidemic periods in the State of Pará, in Northern Brazil, we classify the donors (symptomatic or asymptomatic) and the possibility of this screening being carried out in a sample pool.
Synthesis and Characterization of Highly Efficient Oil-Water Separation, Recyclable, Magnetic Particles CoFe2O4/SDB
Authors: Liu Cailin , Yu Li , Fan Yue , Li Chang , Ren Xianyan , Yang Haijun , Lin Shudong 
Published: June 2021

Abstract
In the oil-water separation, the difficulty to recover and low hydrophobicity are key limitation factors for practical applications. In this paper, we design Cobalt ferrite hybird Polystyrene divinylbenzene microspheres (CoFe2O4/SDB), which were conducted through in-situ suspension copolymerization. The CoFe2O4 is prepared by low heat solid phase sol-gel method. It had been found that the CoFe2O4/SDB have a spherical structure, good adsorption behavior, highly hydrophobicity and even superhydrophobicity. The adsorption capacity of CoFe2O4/SDB composites could absorb kerosene up to 6 times of its own weight. Interestingly, kerosene can be easily separated from the surface of CoFe2O4/SDB particles with ultrasonic operation. CoFe2O4/SDB particles can still maintain good hydrophobicity and adsorption capacity of kerosene after 11 cycles after drying. With in situ polymerization of St、DVB and CoFe2O4, CoFe2O4/SDB as a promising absorbent of kerosene which has great potential in application of oil-water separation.
Challenge Non-Communicable Diseases Programs in Iran: A Qualitative Study
Authors: Ataollahi Fatemeh , Vali Leila , Amiresmaili Mohammadreza , Nakhaee Nouzar , Okhovati Maryam 
Published: June 2021

Abstract
Background

One of the effective strategies to prevent and control Non- communicable diseases risk factors is to identify priorities and problems of Non-communicable programs. this study was conducted to identify the challenges of non-communicable diseases programs in order to improve programs development.
Methods

This study was a qualitative study which was carried out in 5 universities of medical sciences and the Ministry of Health in 2019 year. The sampling method was purposive and the participants include specialists in the field of NCD who have executive and research experience in this field. Data were collected through 40 individual interviews with health professionals and care providers. Data were analyzed by content analysis using CIPP model.
Results

In the present study, problems of non-communicable disease programs were identified in 4 main categories, 12 sub-categories including inadequate participation of all levels of service delivery in program development, needs assessment, budget allocation, data and program evaluation system.
Conclusions

Some problems of non-communicable diseases programs included inadequacy of needs assessment, lack of prioritization based on local and regional conditions, program evaluation system. Paying attention to the mentioned points will help to solve underlying problems and improve the processes of developing, implementing and evaluating programs.
Mini-temporal approach as an alternative to the classical pterional approach for resective temporal region surgeries: Technical note
Authors: Kudulaiti Nijiati , Liu Feili , Hameed N. U. Farrukh , Wang Peng , Zhang Jie , Feng Rui , Wu Jinsong 
Published: June 2021

Abstract
Background

Classical pterional appoach for temporal surgeries may cause atrophy and dysfunction of temporalis, injury to the facial nerve, as well as unnecessary cortical exposure. As an alternative to the classical pterional approach for such surgeries, we hereby describe an mini-temporal approach which reduces these risks and proven to be practical in neurological surgeries.
Methods

In the mini-temporal incision design, the frontal end of the incision never surpassed the hairline at the level of temporal line, and a one-layer skin-galea-muscle flap was detached from the cranium, effectively avoiding the injuries of facial nerve. The surgical bone window was completely located underneath the temporalis muscle, allowing it to be completely repositioned postoperatively.
Results

We demonstrated the application of mini-temporal approach in a variety of temporal region tumors, which can be applied to complete successful resective surgeries while effectively reducing injuries to extra-temporal cortex, temporalis and facial nerve. There were no postoperative complications related to extra-temporal cortical damage, atrophy of temporalis or injury to the facial nerve.
Conclusion

The mini-temporal approach can effectively shorten the time of craniotomy and closure, decrease the size of bony removal, increase the restoration of temporalis during closure and lower the chance of facial nerve injury. Therefore, it improves cosmetic outcomes and reduces the risk of unintentional extra-temporal cortical injury, which fully embodies the minimally invasive principle in neurosurgery.