bluuebunny/crossref_metadata_2025_split huggingface dataset:
APC Overexpression Suppresses the Pyroptosis of Subarachnoid Hemorrhage Model Cells through Regulating NLRP3 Inflammasome Pathway
Authors: Yan Ai , Pan Xuyan , Wen Xianqiang , Nie Xiaohu , Li Yuntao 
Published: June 2021

Abstract
Subarachnoid hemorrhage (SAH) is a kind of hemorrhagic stroke with high mortality. Activated protein C (APC) was implicated to play a neuroprotective role in central nervous system diseases. However, its role in SAH remains unclear. Our study aims to investigate the role of APC and its regulatory mechanism in SAH. The SAH rat model was constructed by internal carotid artery puncture. The SAH cell model was established by the application of oxygen hemoglobin. ELISA was performed to detect the level of cytokines. Flow cytometry was used to detect the population of pyroptosis cells. Neurological functions of rats were estimated using modified Garcia scoring and balance beam test. SAH hemorrhage was estimated using modified Sugawara's scoring. APC was significantly increased and NLRP3 was decreased in SAH rat model in a time-dependent manner. The application of APC recombinant protein 3K3A-APC could notably ameliorate SAH hemorrhage and improve neurological functions. Besides, 3K3A-APC could inhibit pyroptosis in a dose-dependent manner in SAH cell model. Moreover, the inhibition of NLRP3 could reverse the effects induced by the knockdown of APC. Our study revealed that APC could ameliorate SAH-induced EBI by suppressing pyroptosis via inhibiting NLRP3 inflammasome, which would provide a novel strategy for the treatment of SAH.
The Involvement of the Fcεri Signaling Pathway in the Pathogenesis of Lacrimal Gland Benign Lymphoepithelial Lesions
Authors: Li Jing , Liu Rui , Sun Mei , Wang Jinjin , Wang Nan , Zhang Xuan , Ge Xin , Ma Jianmin 
Published: June 2021

Abstract
Objective: Benign lymphoepithelial lesion of lacrimal gland (LGBLEL) is a common orbital inflammatory disease with unknown pathogenesis. T his paper analyzed the role of the FcepsilonRI (FcεRI) signaling pathway in the pathogenesis of LGBLEL.Methods: Transcriptome sequencing and proteome sequencing were performed on LGBLEL and orbital CH diag nosed by histopathology in Beijing Tongren Hospital, Capital Medical University, between July 2010 and October 2013. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis were used to jointly analyze the differentially expressed ge nes and proteins related to FcεRI signaling pathway. Four LGBLEL and three orbital CH diagnosed by histopathology in Beijing Tongren Hospital, Capital Medical University, between October 2018 and August 2019 were randomly selected as the experimental group and the control group, respectively. RT PCR, immunohistochemical staining, and western blotting were used to verify the genes and proteins related to the FcεRI signaling pathway.Results: Combined transcriptome and proteome analysis showed that the FcεRI signaling pathway was up regulated in LGBLEL (P=0.0040), and that the important proteins such as SYK, p38, JNK, PI3K, and ERK were highly expressed in LGBLEL tissues. RT PCR results showed that the mRNA expression levels of SYK, p38, JNK, PI3K, and ERK wer e significantly increased in the LGBLEL group (P=0.0066, P=0.0002, P=0.0003, P<0.0001, P<0.0001, respectively). Immunohistochemical staining results showed that the protein expression levels of SYK, p38, JNK, PI3K, and ERK in LGBLEL tissues were significan tly higher than in orbital CH. Western Blotting showed that the protein contents of p SYK, p p38, p JNK, p PI3K, and p ERK were significantly higher than in orbital CH (P=0.0169, P=0.0074, P=0.0046, P=0.0157, P=0.0156, respectively). Conclusion: The genes and proteins related to the FcεRI signaling pathway are up regulated in LGBLEL, indicating that the FcεRI signaling pathway participates in the pathogenesis of LGBLEL.
Using Bronchoalveolar Lavage Fluid for Active Pulmonary Tuberculosis Laboratory Diagnosis
Authors: Han Mei , Guo Jian-Qiong , Yan Xiao-Feng , Yue Wu-Yang , Qiu Qian , Wang Le-Le , Sun Qiang-Zhong , Jiang Ling , Li Tong-Xin , Fan You-Ming , Yang Song 
Published: June 2021

Abstract
Objective: To evaluate the diagnostic efficiency of bronchoalveolar lavage fluid (BALF) for MycobacteriumTuberculosis (MTB) infection using laboratory methods. Methods: A retrospective study was conducted in patients diagnosed with active pulmonary tuberculosis (APTB) and lacking sputum quality/quantity. BALF collected during the operation processes of Electric bronchoscopy were tested using Ziehl-Neelsen staining acid-fast bacilli smear microscopy (Z-N-AFB-SM), GeneXpert MTB/RIF（Xpert），loop-mediated isothermal amplification (LAMP), or culturing with BACTEC™ Mycobacterial Growth Indicator Tube™ 960 (MGIT). Chi-square test was used for statistic analysis. Results: 331 suspected APTB patients were enrolled in this study. 224 of them were sputum-scarce. 89 were sputum-sufficient andnegative in both Z-N-AFB-SM and MGIT 960 testing. Of the sputum sufficient patients, BALF-testing confirmed APTB diagnosis in 20.2% (18/89) via Z-N-AFB-SM, and 53.0% (35/89) via MGIT. The total positive rates of BALF testing via four aforementioned methods were 18.2% (57/313), 66.4% (168/253), 61.0% (83/136) and 48.2% (140/290) respectively. The positive rate of MTB discovered in BALF collected by well-trained respiratory physicians are significantly higher than those collected by anesthetists (χ2=22.48, P<0.01). Total adverse events incidence of BAL was 1.9% (6/313). Conclusion: BALF has a similar sensitivity and specificity for APTB laboratory diagnosis. It can be used as a complementary diagnostic method for APTB when sputum availability is poor. The proficiency of BALF collection is an important factor affecting the detection results.
Ex Vivo Microsphiltration Profile of Plasmodium Falciparum Infected Red Blood Cells in Patients with Malaria in Kéniéroba, Mali: Exploring the Spleen in Vivo Retention Function
Authors: KEITA Bourama , Diakité Seidina A.S. , Guindo Agnes M. , Konaté Drissa S. , Traoré Karim , Sangaré Modibo , Diawara Sory I. , Sanogo Ibrahim , Diarra Bakaina , Diakité Mahamadou 
Published: June 2021

Abstract
Malaria pathophysiology is not still fully understood. The main mechanisms of malaria involve the synergistic interactions between host and parasite. Although, the role of the spleen has been mentioned in various clinical forms of malaria, a supportive clinical evidence is still needed. We conducted a pilot study to determine the impact of the spleen functional state in different clinical forms of malaria.Ex vivo microsphiltration was used to assess the splenic function in patients received during routine consultation with malaria at the Kéniéroba health center, a ​​high malaria endemic area in Mali.A total of 25 patients were enrolled for microsphiltration. Two patients (8%) had a no palpable spleen (Hackett stage 0), 22 patients (88%) had a palpable spleen with at deep inspiration (Hackett stage 1) and only one patient (4%) presented a palpable spleen (Hackett stage 2). Parasitaemia ranged from 5360 trophozoites/µl to 342720 trophozoites/µl with a mean parasitemia of 50774 trophozoites/µl ± 65540 trophozoites/µl; the mean hemoglobin rate was 11.2 ± 1.2 g/dl with the extremes of 8.7 g/dl and 13.4 g/dl. The retention rate of the infected red blood cell ranged from 11.11% to 94.44% with an average of 65.4% ± 23.7%. A higher ex vivo retention rate of infected red blood cells was observed in patients with Hackett stages greater than or equal to 1 (p= 0.03). This pilot study proved the feasibility of the exploration of the spleen filtering function in malaria patients using the ex vivo microsphiltration.
Effect of Solvents on Bioactive Compounds and Antioxidant Activity of Padina Tetrastromatica and Gracilaria Tenuistipitata Seaweeds Collected From Bangladesh
Authors: Sobuj Mohammad Khairul Alam , Islam Md. Ariful , Islam Md. Shoebul , Islam Md. Mohidul , Mahmud Yahia , Rafiquzzaman S.M. 
Published: June 2021

Abstract
Seaweeds are now recognized as a treasure of bioactive compounds. However, the seaweed of Bangladesh is still unexplored. So, this study was designed to explore the secondary metabolites and antioxidant activities of solvent extracts of Padina tetrastromatica and Gracilaria tenuistipitata. Phytochemical screening and Fourier transform infrared (FTIR) confirm the diverse type of bioactive compounds. Antioxidant activity of extracts were evaluated by 1, 1-diphenyl-2- picrylhydrazyl (DPPH), 2, 2-Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), reducing power (RP), phosphomolybdenum and hydrogen peroxide assays. Here, methanolic extract of P. tetrastromatica showed the highest amount of total phenolic content (85.61 mg of GA/g), total flavonoid content (41.77 mg of quercetin/g), which showed as potent antioxidant properties confirmed by DPPH (77.07%), ABTS (77.65%), RP (53.24 mg AAE/g), phosphomolybdenum (31.58 mg AAE/g) and hydrogen peroxide (67.89%) assays followed by ethanol and water. This study concluded that bioactive compound and antioxidant activities are dose and solvent-dependent and seaweed type.
Hospital Costs of Immunopreventable Diseases in the Economically Active Population in Brazil
Authors: Costa Élide Sbardellotto Mariano , Hyeda Adriano , Maluf Eliane M C P 
Published: June 2021

Abstract
Background: Immunopreventable diseases are a public health reality in Brazil and worldwide. Methods: A population, observational, descriptive, retrospective study was conducted with secondary information from DATASUS to discriminate the hospitalizations associated with immunopreventable diseases in Brazil and their care costs, within the Scope of the SUS, between 2008 and 2018, in the economically active population (20 to 59 years). Results: It was analyzed for 457,479 hospitalizations, total of 127,746 hospitalizations (27.92% of all hospitalizations) were observed for immunopreventable diseases in the adult population, totaled R$115,682,097.54 (29.72% of the total values. The trend analysis of the time series of hospitalizations in this population showed a stationary trend. Conclusions: It were identified an opportunity of increasing the immunization coverage in the workforce population, for avoided hospitalizations and their costs for the health system.
The Implementation of Welfare Technology in Municipal Care: A Qualitative Study of Procurement Practices in Sweden
Authors: Kuoppamäki Sanna 
Published: June 2021

Abstract
Background: Welfare technology has been launched as a concept to accelerate digital transformation in care services, but the implementation of these technologies is still hindered by organisational resistance, lack of infrastructure, and juridical and ethical issues. This paper investigates the decision-making among municipal actors in the implementation of welfare technology in a specific task that is procurement. The study explores the perceptions and negotiations in purchasing welfare technology in each stage of the procurement model revealing the impact of technical, economic, juridical and ethical competence on the mapping and planning, procurement, implementation and management of welfare technology. Methods: The study presents empirical findings from a qualitative interview study for municipal actors in Sweden. Semi-structured interviews were gathered among procurement managers, IT managers, and managers in social administrators in three different municipalities in 2020 (N=8). Inductive content analysis and systematic categorisation were employed resulting to 17 sub-categories, 9 generic categories and 3 main categories of procurement practices. Results: Challenges in the procurement practices occur in all stages of the procurement model. In mapping and planning, municipal actors outline barriers the need analysis, requirement specification and market analysis. In procurement stage, economic resources, standardisation and interoperability hinder the procurement process. Implementation and management is complicated by supplier assessment, legislation, cross-organisational collaboration and political strategy. Building on these findings, the study defines the procurement competence to consist of technical, economic, juridical and ethical expertise to assess and evaluate welfare technology. Technical and ethical competence is needed in early stages of the procurement, whereas juridical and economic competence relates to later stages of the model.Conclusions: Procurement competence is associated with the implementation of welfare technology in 1) assessment of the end-users needs, 2) estimation of the costs and benefits of welfare technology, and 3) management of the juridical and legislation issues in data management. Economic and juridical decisions to purchase welfare technology are not value-neutral, but rather associated with socially shared understandings of technological possibilities in care provision. Optimisation of procurement process requires a combination of capabilities to implement welfare technology that meet the demands and needs of end-users.
Automated Guided Method for Roadheader fuselage
Authors: Chen Hongyue , Yang Wei , Zhang Desheng , Xiao Song 
Published: June 2021

Abstract
Autonomous Navigation of roadheader is a hot topic in recent research. The subject of this paper mainly focuses on the motion control of the roadheader fuselage. Because of the particularity of its work content and operational environment, the motion control is different from that of the traditional differential-drived vehicle. This paper introduces a method to detect the position and posture of roadheader fuselage in harsh underground environment, which lays a foundation for motion control. In addition, a path planning method based on prediction model is proposed to reduce the control effort while ensuring enough control accuracy. Then the kinematics characteristics of roadheader fuselage with and without the path planning process are analyzed and compared by simulation. The result shows that the proposed method makes the movement of the roadheader smoother and more suitable for the tunneling process compared with the conventional method.
Model Tests and Numerical Simulations of Grouted Connections in Offshore Wind Turbines Under Low Circumferential Repeated Loads
Authors: Zhong Weiqiu , Li Wuxu , Yin Rui 
Published: June 2021

Abstract
Background: Grouted connection sections are widely used to connect the support structure of offshore wind turbines to the foundation, and their mechanical performance is crucial to the reliability of the whole wind turbine. In order to find a suitable stress evaluation method for the grouted connection section, finite element simulation analysis is conducted in this paper.Methods: The stress analysis of the grouted connection section under different frequency loads was explored by establishing a scaled-down model and numerical analysis, and the influence of various parameters on the mechanical properties of the connection section was investigated and its engineering applicability was evaluated.Results: The results of scaling tests and numerical analysis showed that the specimens without shear keys in the axial direction had the worst engineering practicality, and the specimens with shear keys in the taper had the best engineering applicability.Conclusions: The results of the reduced-scale tests and numerical analysis can provide a reference for the design of grouted joint sections, and the relationship between them can be applied to the preliminary evaluation of the mechanical properties of grouted joint sections under low circumferential repeated loads.
The Relationship Between Ultrasound and Semen Parameters in Male Infertility: Size and Elasticity Matters!
Authors: Pásztor Norbert , Veréb Dániel , Király István , Kincses Zsigmond Tamás , Palkó András , Németh Gábor , Bajory Zoltán , Fejes Zsuzsanna 
Published: June 2021

Abstract
ObjectivesTesticular ultrasound is important in the assessment of male infertility and adding strain elastography to the protocol can help clarify the underlying etiology. Material and Methods78 participants (64 with abnormal, 14 with normal semen parameters) underwent sperm analysis and testicular US including strain elastography. Semen parameters, testicular volumes, strain elastography parameters were compared between groups using independent samples T-tests, least squares regression, Spearman’s rank correlation. ResultsParticipants with abnormal semen parameters exhibited reduced testicular volumes (left: p<0.0003, right: p<0.0004) and epididymis diameters (left: p<0.01, right: p<0.006), and higher strain ratios (left: p<0.006, right: p<0.001). The first PLS latent variable explained a significant amount of variance in semen parameters (p<0.0002) and showed that bilateral strain ratios, left testicular volume and left appendix size were the most important predictors. Elevated strain ratios came with reduced sperm cell number (right: R=-0.45, p<0.0001; left: R=-0.46, p<0.0001), sperm concentration (right: R=-0.46, p<0.0001; left: R=-0.48, p<0.0001), total (right: R=-0.39, p<0.001) and progressive motility (right: R= -0.4, p<0.001), non-motility (left: R=-0.37, p<0.003) normal morphology (right: R=-0.37, p<0.003). ConclusionWe confirm previous reports of correlation between sperm parameters, testis volume and epididymis diameter. Furthermore, we show that strain elastography parameters are strong predictors of semen parameters.
MRI Detection of Active Sacroiliitis in First Degree Relatives of Ankylosing Spondylitis Patients with Clinical and Laboratory Correlations
Authors: Obaid Haron , Milosavljevic Stephan , Okpalauwaekwe Udoka , Bath Brenna , Trask Catherine , Leswick David , Bussey Melanie D. , Katselis George , Loeppky Rhonda , Lovo Stacey , Taylor-Gjevre Regina , Nair Bindu 
Published: June 2021

Abstract
Background. Detection of ankylosing spondylitis (AS) in the preclinical stage could help prevent long term morbidity in this patients’ population. The aim of this study was to examine the prevalence of active sacroiliitis in first-degree relatives of AS patients using MRI with clinical and laboratory correlations as these patients may benefit from MRI screening and early treatment.Methods. Seventeen first-degree relatives of AS patients were recruited prospectively. AS screening questionnaires (Ankylosing Spondylitis Disease Activity Score, Bath Ankylosing Spondylitis Disease Activity Index & Visual Analogue Scale), blood tests (C-Reactive Protein, HLA-B27), and an MRI of the SIJs were taken. Two musculoskeletal radiologists interpreted the MRI scans, and two physiotherapists applied four symptom provocation tests (Gaenslen's test, posterior pelvic pain provocation test, Patrick's Faber (PF) test and palpation of the long dorsal SIJ ligament test), and two functional movement tests (active straight leg raise and Stork test). Results. Seven (41%) of the 17 participants demonstrated MRI evidence of active sacroiliitis. Of the 7 participants with active sacroiliitis, two (29%) had no history of recent low back pain (LBP), two (29%) had negative HLA-B27, and one (14%) participant had neither back pain nor positive HLA-B27. The Cohen's Kappa score for the interobserver agreement between the radiologists was 1.00 (p-value <0.0001). Despite fair to strong between therapist agreement for the physical test outcomes (Kappa 0.26 to 1.00), the physical test results per se did not have any predictive association with a positive MRI.Conclusions. MRI detected active sacroiliitis in 41% of first-degree relatives of AS patients. The lack of a history of prior LBP or positive HLA-B27 in active sacroiliitis participants might suggest that MRI screening for this high-risk population is warranted; however, further larger studies are needed to help elucidate its cost-effectiveness and long-term benefits.
Quantitative Analysis of Lysine Acetylation in Vero Cells Infected With Peste Des Petits Ruminants Virus
Authors: Meng Xuelian , Zhu Xueliang , Zhang Rui , Zhang Zhidong 
Published: June 2021

Abstract
Background: Peste des petits ruminants virus (PPRV) is a negative-stranded RNA virus belonging to the Paramyxoviridae family and causes acute, highly contagious disease in small ruminants. Lysine acetylation plays central role in regulating gene expression. However, the extent and function of lysine acetylation in host cells during PPRV infection remains unknown. Methods: Lysine acetylation of PPRV-infected Vero cells was tested and differentially expressed lysine acetylation was found. The acetylated peptides were enriched using specific antibody and labeled with demethylation. Proteins with acetylation sites were identified. Subsequently, intensive bioinformatics analysis of succinylome of PPRV-infected Vero cells was were performed. In this study, intensive proteomic quantification analysis of the proteome and acetylome of PPRV-infected Vero cells was performed using dimethylation labeling-based quantitative proteomics. Results: We identified 4729 cellular proteins and 1068 proteins with 2641 modification sites quantifiable detected by mass spectrometry, of which 304 proteins with 410 acetylation sites were significantly acetylated in response to PPRV infection. Bioinformatics analyses revealed that the differentially acetylated proteins mainly participated in carbohydrate catabolic and DNA metabolic process, and were associated with multifarious functions, suggesting that intracellular activities were extensively changed after PPRV infection. Protein-protein interaction (PPI) network of the identified proteins further indicated that a variety of chaperone and ribosome processes were modulated by acetylation. Conclusions: To our knowledge, this is the first study on acetylome in host cell infected with PPRV. It provides an important baseline to future study the roles of acetylation in the host response to PPRV replication.
Reproductive Factors and Risk of Dementia in Natural Postmenopausal Women: a Cross-sectional Study
Authors: Gan Jinghuan , Chen Zhichao , Liu Shuai , Wang Fei , Wang Xiaodan , Hu Wenzheng , Du Xiaoshan , Zhu Han , Ma Lingyun , Shi Zhihong , Ji Yong 
Published: June 2021

Abstract
Background: Women comprise more than half of people suffering cognitive impairment. This study aims to evaluate the association or interaction between reproductive factors and the risk of dementia in Chinese women with natural menopause.Methods: The cross-sectional study was conducted in 112 community primary health care centers in rural northern China between April 2019 and January 2020. A total of 4,275 women aged ≥ 65 years who had natural menopause were included. Reproductive factors were recorded by self-report. Reproductive period was calculated as age at menopause minus age at menarche.Results: Compared to those without dementia, women with dementia were significantly older at menarche and younger at menopause; had significantly shorter duration of reproductive period; and had more pregnancies and parities. There were 0.757, 0.698, and 0.708 times to get dementia for women who experienced reproductive periods with 31-33 years, 34-36 years, and ≥ 37 years, respectively, as compared with ≤ 30 years. Reproductive period could positively predict MMSE score (β = 0.112) and negative prediction of the number of parities (β = -0.449); the number of parities could negatively predict MMSE score (β = -0.851) as well.Conclusions: Longer duration of reproductive period, directly or through lower number of pregnancies/parities indirectly, lowers the risk of dementia in late life.