bluuebunny/crossref_metadata_2025_split huggingface dataset:
Nighttime compression supports improved self‐management of breast cancer–related lymphedema: A multicenter randomized controlled trial
Authors: McNeely Margaret L. , Dolgoy Naomi D. , Rafn Bolette Skjodt , Ghosh Sunita , Ospina Paula A. , Al Onazi Mona M. , Radke Lori , Shular Mara , Kuusk Urve , Webster Marc , Campbell Kristin L. , Mackey John R. 
Published: October 2021

BackgroundLymphedema is a prevalent long‐term effect of breast cancer treatment associated with reduced quality of life. This study examined the efficacy of nighttime compression as a self‐management strategy for women with chronic breast cancer–related lymphedema.MethodsTh authors conducted a parallel 3‐arm, multicenter, randomized trial. Women were recruited from 3 centers in Canada and randomized to group 1 (daytime compression garment alone [standard care]), group 2 (daytime compression garment plus nighttime compression bandaging), or group 3 (daytime compression garment plus the use of a nighttime compression system garment). The primary outcome was the change in excess arm volume from the baseline to 12 weeks. Participants from all groups used a nighttime compression system garment from weeks 13 to 24.ResultsOne hundred twenty women were enrolled, 118 completed the randomized trial, and 114 completed the 24‐week follow‐up. The rates of adherence to nighttime compression were 95% ± 15% and 96% ± 11% in the compression bandaging and nighttime compression system groups, respectively. After the intervention, the addition of nighttime compression was found to be superior to standard care for both absolute milliliter reductions (P = .006) and percentage reductions (P = .002) in excess arm lymphedema volume. Significant within‐group changes were seen for quality of life across all groups; however, no between‐group differences were found (P > .05).ConclusionsThe trial demonstrated a significant improvement in arm lymphedema volume from the addition of nighttime compression whether through the application of compression bandaging or through the use of a nighttime compression system garment.Lay Summary

Lymphedema is swelling that occurs in the arm on the side of the surgery for breast cancer.

Lymphedema occurs in approximately 21% of women.

Lymphedema tends to worsen over time and can result in recurrent infections in the arm, functional impairment, and pain.

Currently, treatment consists of intensive treatments to reduce the swelling followed by regular use of a compression sleeve during the day.

This study examined and found a benefit from the addition of nighttime compression (whether through self‐applied compression bandaging or through the use of a nighttime compression system garment) to the use of a daytime compression sleeve.
Oxytocin‐like receptor expression in evolutionarily conserved nodes of a vocal network associated with male courtship in a teleost fish
Authors: Schuppe Eric R. , Zhang Melissa D. , Perelmuter Jonathan T. , Marchaterre Margaret A. , Bass Andrew H. 
Published: October 2021

AbstractNeuropeptides, including oxytocin‐like peptides, are a conserved group of hormones that regulate a wide range of social behaviors, including vocal communication. In the current study, we evaluate whether putative brain sites for the actions of isotocin (IT), the oxytocin (OT) homolog of teleost fishes are associated with vocal courtship and circuitry in the plainfin midshipman fish (Porichthys notatus). During the breeding season, nesting males produce advertisement calls known as “hums” to acoustically court females at night and attract them to nests. We first identify IT receptor (ITR) mRNA in evolutionarily conserved regions of the forebrain preoptic area (POA), anterior hypothalamus (AH), and midbrain periaqueductal gray (PAG), and in two topographically separate populations within the hindbrain vocal pattern generator– duration‐coding vocal prepacemaker (VPP) and amplitude‐coding vocal motor nuclei (VMN) that also innervate vocal muscles. We also verify that ITR expression overlaps known distribution sites of OT‐like immunoreactive fibers. Next, using phosphorylated ribosomal subunit 6 (pS6) as a marker for activated neurons, we demonstrate that ITR‐containing neurons in the anterior parvocellular POA, AH, PAG, VPP, and VMN are activated in humming males. Posterior parvocellular and magno/gigantocellular divisions of the POA remain constitutively active in nonhumming males that are also in a reproductive state. Together with prior studies of midshipman fish and other vertebrates, our findings suggest that IT‐signaling influences male courtship behavior, in part, by acting on brain regions that broadly influence behavioral state (POA) as well as the initiation (POA and PAG) and temporal structure (VPP and VMN) of advertisement hums.
A color channel based on multiple Random Forest coupled with a computer vision technique for the detection and prediction of Sudan dye‐I adulteration in turmeric powder
Authors: Mandal Dipankar , Chatterjee Arpitam , Tudu Bipan 
Published: October 2021

AbstractAdulterants can cause different health hazards upon prolonged consumption, but it is difficult to detect with human eyes. Non‐destructive turmeric adulteration detection is a challenging research area. The existing adulteration detection processes are largely instrumental and analytical with high accuracy but include limitations like long testing time, expensiveness, and lack of mobility. This work reports a new computer vision framework, which can simultaneously detect the presence of adulteration and predict the possible percentage of adulteration addressing the stated limitations. The scope has been remained to screening of Sudan dye‐I adulteration in turmeric powder. An in‐house database prepared with images of pure and adulterated turmeric powder samples has been used for experimentation. Random Forest algorithm has been employed for both classification and prediction. The model has been validated with standard internal and external validation methods to assess the stability and generalization potential of the model to avoid over‐ and under‐fitting problems. The results of classification show that the presented framework can provide more than 99% accuracy in detection while high correlation coefficient (R2) value in the tune of .99 for prediction. The novelty of the work is its simple histogram‐based color feature extraction, development of ensemble Random Forest prediction model that resulted in high accuracy and development of a faster, non‐invasive, less‐expensive, and validated screening method for adulterated turmeric powder that can be considered as a potential immediate screening method in the supply chain of powdered spices prior to confirmatory testing methods following two‐tiered food fraud testing approach.
How many replicates to accurately estimate fish biodiversity using environmental DNA on coral reefs?
Authors: Stauffer Salomé , Jucker Meret , Keggin Thomas , Marques Virginie , Andrello Marco , Bessudo Sandra , Cheutin Marie‐Charlotte , Borrero‐Pérez Giomar Helena , Richards Eilísh , Dejean Tony , Hocdé Régis , Juhel Jean‐Baptiste , Ladino Felipe , Letessier Tom B. , Loiseau Nicolas , Maire Eva , Mouillot David , Mutis Martinezguerra Maria , Manel Stéphanie , Polanco Fernández Andrea , Valentini Alice , Velez Laure , Albouy Camille , Pellissier Loïc , Waldock Conor 
Published: October 2021

AbstractQuantifying fish species diversity in rich tropical marine environments remains challenging. Environmental DNA (eDNA) metabarcoding is a promising tool to face this challenge through the filtering, amplification, and sequencing of DNA traces from water samples. However, because eDNA concentration is low in marine environments, the reliability of eDNA to detect species diversity can be limited. Using an eDNA metabarcoding approach to identify fish Molecular Taxonomic Units (MOTUs) with a single 12S marker, we aimed to assess how the number of sampling replicates and filtered water volume affect biodiversity estimates. We used a paired sampling design of 30 L per replicate on 68 reef transects from 8 sites in 3 tropical regions. We quantified local and regional sampling variability by comparing MOTU richness, compositional turnover, and compositional nestedness. We found strong turnover of MOTUs between replicated pairs of samples undertaken in the same location, time, and conditions. Paired samples contained non‐overlapping assemblages rather than subsets of one another. As a result, non‐saturated localized diversity accumulation curves suggest that even 6 replicates (180 L) in the same location can underestimate local diversity (for an area <1 km). However, sampling regional diversity using ~25 replicates in variable locations (often covering 10 s of km) often saturated biodiversity accumulation curves. Our results demonstrate variability of diversity estimates possibly arising from heterogeneous distribution of eDNA in seawater, highly skewed frequencies of eDNA traces per MOTU, in addition to variability in eDNA processing. This high compositional variability has consequences for using eDNA to monitor temporal and spatial biodiversity changes in local assemblages. Avoiding false‐negative detections in future biomonitoring efforts requires increasing replicates or sampled water volume to better inform management of marine biodiversity using eDNA.
Habitat size influences community stability
Authors: Greig Hamish S. , McHugh Peter A. , Thompson Ross M. , Warburton Helen J. , McIntosh Angus R. 
Published: October 2021

AbstractMechanisms linked to demographic, biogeographic, and food‐web processes thought to underpin community stability could be affected by habitat size, but the effects of habitat size on community stability remain relatively unknown. We investigated whether those habitat‐size‐dependent properties influenced community instability and vulnerability to perturbations caused by disturbance. This is particularly important given that human exploitation is contracting ecosystems, and abiotic perturbations are becoming more severe and frequent. We used a perturbation experiment in which 10 streams, spanning three orders of magnitude in habitat size, were subjected to simulated bed movement akin to a major flood disturbance event. We measured the resistance, resilience, and variability of basal resources, and population and community‐level responses across the stream habitat‐size gradient immediately before, and at 0.5, 5, 10, 20, and 40 d post‐disturbance. Resistance to disturbance consistently increased with stream size in all response variables. In contrast, resilience was significantly higher in smaller streams for some response variables. However, this higher resilience of small ecosystems was insufficient to compensate for their lower resistance, and communities of smaller streams were thus more variable over time than those of larger streams. Compensatory dynamics of populations, especially for predators, stabilized some aspects of communities, but these mechanisms were unrelated to habitat size. Together, our results provide compelling evidence for the links between habitat size and community stability, and should motivate ecologists and managers to consider how changes in the size of habitats will alter the vulnerability of ecosystems to perturbations caused by environmental disturbance.
Risk factors for left ventricular dysfunction in adulthood: role of low birth weight
Authors: Huynh Quan L. , Venn Alison J. , Magnussen Costan G. , Yang Hong , Dwyer Terence , Marwick Thomas H. 
Published: October 2021

AbstractAimsThis study aimed to determine the relationship of low birth weight (LBW) with adult cardiac structure and function and investigate potential causal pathways.Methods and resultsA population‐based sample of 925 Australians (41.3% male) were followed from childhood (aged 7–15 years) to young adulthood (aged 26–36 years) and mid‐adulthood (aged 36–50 years). Left ventricular (LV) global longitudinal strain (GLS, %), LV mass index (LVMi, g/m2.7), LV filling pressure (E/e′), and left atrial volume index (g/m2) were measured by transthoracic echocardiography in mid‐adulthood. Birth weight category was self‐reported in young adulthood and classified as low (≤5 lb or ≤2270 g), normal (5–8 lb or 2271–3630 g), and high (>8 lb or >3630 g). Of the 925 participants, 7.5% (n = 69) were classified as LBW. Compared with participants with normal birth weight, those with LBW had 2.01‐fold (95% confidence interval: 1.19, 3.41, P = 0.009) higher risks of impaired GLS (GLS > −18%) and 2.63‐fold (95% confidence interval: 0.89, 7.81, P = 0.08) higher risks of LV hypertrophy (LVMi > 48 g/m2.7 in men or >44 g/m2.7 in women) in adulthood, independent of age, sex, and any socio‐economic factors. Participants with LBW significantly increased body fat from childhood to adulthood relative to their peers and had greater levels of triglycerides, fasting glucose, and arterial stiffness in adulthood. These risk factors were the strongest mediators and explained 54% of the LBW effect size on adult GLS and 33% of the LBW effect size on LVMi. The remaining of these associations was independent of any of the measured risk factors.ConclusionsLow birth weight was associated with impaired cardiac structure and function in mid‐adulthood. This association was only partially explained by known risk factors.
Low‐cost polymer‐film spiral inertial microfluidic device for label‐free separation of malignant tumor cells
Authors: Wang Cailian , Chen Yan , Gu Xuyu , Zhang Xiuxiu , Gao Chanchan , Dong Lijun , Zheng Shiya , Feng Shicheng , Xiang Nan 
Published: October 2021

AbstractWe developed a low‐cost polymer‐film spiral inertial microfluidic device for the effective size‐dependent separation of malignant tumor cells. The device was fabricated in polymer films by rapid laser cutting and chemical bonding. After fabricating the prototype device, the separation performance of our device was evaluated using particles and cells. The effects of operational flow rate, cell diameter, and cell concentration on the separation performance were explored. Our device successfully separated tumor cells from polydisperse white blood cells according to their different migration modes and lateral positions. Then, the separation of rare cells was carried out using the high‐concentration lysed blood spiked with 200 tumor cells. Experimental results showed that 83.90% of the tumor cells could be recovered, while 99.87% of white blood cells could be removed. We successfully employed our device for processing clinical pleural effusion samples from patients with advanced metastatic breast cancer. Malignant tumor cells with an average purity of 2.37% could be effectively enriched, improving downstream diagnostic accuracy. Our device offers the advantages of label‐free operation, low cost, and fast fabrication, thus being a potential tool for effective cell separation.
What are the current and projected future cost and health‐related quality of life implications of scaling up cognitive stimulation therapy?
Authors: Knapp Martin , Bauer Annette , Wittenberg Raphael , Comas‐Herrera Adelina , Cyhlarova Eva , Hu Bo , Jagger Carol , Kingston Andrew , Patel Anita , Spector Aimee , Wessel Audrey , Wong Gloria 
Published: October 2021

AbstractObjectivesCognitive stimulation therapy (CST) is one of the few non‐pharmacological interventions for people living with dementia shown to be effective and cost‐effective. What are the current and future cost and health‐related quality of life implications of scaling‐up CST to eligible new cases of dementia in England?Methods/designData from trials were combined with microsimulation and macrosimulation modelling to project future prevalence, needs and costs. Health and social costs, unpaid care costs and quality‐adjusted life years (QALYs) were compared with and without scaling‐up of CST and follow‐on maintenance CST (MCST).ResultsScaling‐up group CST requires year‐on‐year increases in expenditure (mainly on staff), but these would be partially offset by reductions in health and care costs. Unpaid care costs would increase. Scaling‐up MCST would also require additional expenditure, but without generating savings elsewhere. There would be improvements in general cognitive functioning and health‐related quality of life, summarised in terms of QALY gains. Cost per QALY for CST alone would increase from £12,596 in 2015 to £19,573 by 2040, which is below the threshold for cost‐effectiveness used by the National Institute for Health and Care Excellence (NICE). Cost per QALY for CST and MCST combined would grow from £19,883 in 2015 to £30,906 by 2040, making it less likely to be recommended by NICE on cost‐effectiveness grounds.ConclusionsScaling‐up CST England for people with incident dementia can improve lives in an affordable, cost‐effective manner. Adding MCST also improves health‐related quality of life, but the economic evidence is less compelling.
Functional transcranial Doppler: Selection of methods for statistical analysis and representation of changes in flow velocity
Authors: Egger Stephan T. , Bobes Julio , Seifritz Erich , Vetter Stefan , Schuepbach Daniel 
Published: October 2021

AbstractIntroductionTranscranial Doppler (TCD) is a method used to study cerebral hemodynamics. In the majority of TCD studies, regression analysis and analysis of variance are the most frequently applied statistical methods. However, due to the dynamic and interdependent nature of flow velocity, nonparametric tests may allow for better statistical analysis and representation of results.MethodThe sample comprised 30 healthy participants, aged 33.87 ± 7.48 years; with 33% (n = 10) females. During a visuo‐motor task, the mean flow velocity (MFV) in the middle cerebral artery (MCA) was measured using TCD. The MFV was converted to values relative to the resting state. The results obtained were analyzed using the general linear model (GLM) and the general additional model (GAM). The fit indices of both analysis methods were compared with each other.ResultsBoth MCAs showed a steady increase in MFV during the visuo‐motor task, smoothly returning to resting state values. During the first 20 seconds of the visuo‐motor task, the MFV increased by a factor of 1.06 ± 0.07 in the right‐MCA and by a factor of 1.08 ± 0.07 in the left‐MCA. GLM and GAM showed a statistically significant change in MFV (GLM:F(2, 3598) = 16.76, P < .001; GAM:F(2, 3598) = 21.63, P < .001); together with effects of hemispheric side and gender (GLM:F(4, 3596) = 7.83, P < .005; GAM:F(4, 3596) = 2.13, P = .001). Comparing the models using the χ2 test for goodness of fit yields a significant difference χ2 (9.9556) = 0.6836, P < .001.ConclusionsBoth the GLM and GAM yielded valid statistical models of MFV in the MCA in healthy subjects. However, the model using the GAM resulted in improved fit indices. The GAM's advantage becomes even clearer when the MFV curves are visualized; yielding a more realistic approach to brain hemodynamics, thus allowing for an improvement in the interpretation of the mathematical and statistical results. Our results demonstrate the utility of the GAM for the analysis and representation of hemodynamic parameters.
Convergence of emerging technologies: Development of a risk-based paradigm for marine mammal monitoring for offshore wind energy operations
Authors: Macrander A. Michael , Brzuzy Louis , Raghukumar Kaustubha , Preziosi Damian , Jones Craig 
Published: October 2021

Abstract
The ability to gather real-time and near real-time data on marine mammal distribution, movement, and habitat use has advanced significantly over the past two decades. These advances have outpaced their adoption into a meaningful, risk-based assessment framework so critically needed to support society's growing demands for a transition to increased reliance on renewable energy. Marine acoustics have the capacity to detect, identify, and locate vocalizations over broad areas. Photogrammetric and image processing increases the ability to visually detect animals from surface or aerial platforms. Ecological models based on long-term observational data coupled with static and remotely sensed oceanographic data are able to predict daily and seasonal habitat suitability. Extensive monitoring around anthropogenic activities, combined with controlled experiments of exposure parameters (i.e., sound), supports better informed decisions on reducing effects. Population models and potential consequence modeling provide the ability to estimate the significance of individual and population exposure. The collective capacities of these emerging technical approaches support a risk ranking and risk management approach to monitoring and mitigating effects on marine mammals related to development activities. The monitoring paradigm related to many offshore energy-related activities, however, has long been spatially limited, situationally myopic, and operationally uncertain. A case evaluation process is used to define and demonstrate the changing paradigm of effective monitoring aimed at protecting living resources and concurrently providing increased certainty that essential activities can proceed efficiently. Recent advances in both technologies and operational approaches are examined to delineate a risk-based paradigm, driven by a diversity of regional data inputs, that is capable of meeting the imperative for timely development of offshore wind energy. Integr Environ Assess Manag 2022;18:939–949. © 2021 The Authors. Integrated Environmental Assessment and Management published by Wiley Periodicals LLC on behalf of Society of Environmental Toxicology & Chemistry (SETAC).
KEY POINTS
This paper makes the case that the national policy and mandate for development of offshore wind resources can be facilitated by the updating of marine mammal monitoring approaches through the incorporation of emerging technologies and a regional risk-based approach. There are currently a number of existing and developing efforts to collect information on marine mammals in the Atlantic Outer Continental Shelf that can be updated and incorporate into a regional monitoring effort through collaboration and integration. The regulatory construct is adaptable to a regional risk-based approach, the implementation of which would require acceptance by regulators and stakeholders and relief for project proponents.
Dehydrated human amniotic membrane regulates tenocyte expression and angiogenesis in vitro: Implications for a therapeutic treatment of tendinopathy
Authors: Moreno Sarah E. , Massee Michelle , Koob Thomas J. 
Published: October 2021

AbstractTendon injuries are among the most common ailments of the musculoskeletal system. Prolonged inflammation and persistent vasculature are common complications associated with poor healing. Damaged tendon, replaced with scar tissue, never completely regains the native structural or biomechanical properties. This study evaluated the effects of micronized dehydrated human amnion/chorion membrane (μdHACM) on the inflammatory environment and hypervascularity associated with tendinopathy. Stimulation of human tenocytes with interleukin‐1 beta (IL1β) induced the expression of inflammatory and catabolic markers, resulting in secretion of active MMPs and type 3 collagen that is associated with a degenerative phenotype. Treatment with μdHACM diminished the effects of IL1β, reducing the expression of inflammatory genes, proteases, and extracellular matrix components, and decreasing the presence of active MMP and type 3 collagen. Additionally, a co‐culture model was developed to evaluate the effects of μdHACM on angiogenesis associated with tendinopathy. Micronized dHACM differentially regulated angiogenesis depending upon the cellular environment in which it was placed. This phenomenon can be explained in part through the detection of both angiogenic protagonists and antagonists in μdHACM. Observations from this study identify a mechanism by which μdHACM regulates inflammatory processes and angiogenesis in vitro, two key pathways implicated in tendinopathic injuries.