bluuebunny/crossref_metadata_2025_split huggingface dataset:
COVID-19 pandemic and the consumption behaviour of branded functional beverages in india: a conceptual framework
Authors: Natarajan Thamaraiselvan , G.R. Jayadevan 
Published: September 2021

Purpose
This conceptual paper aims to study the Corona Virus Disease-19 (COVID-19) pandemic’s impact on branded functional beverages consumption in India. There is a sincere effort to critically review the role of some vital determinants of consumer behaviour and to develop an integrated model for branded functional beverages consumption behaviour.

Design/methodology/approach
A systematic and extensive literature review was performed by searching electronic databases relating to consumer acceptance and purchase behaviour of functional beverages in India from 2000 to 2021. This study also reviewed very recent literature on the potential impact of the COVID-19 pandemic on global consumer traits, buying behaviour, marketing trends and psychographic behaviour.

Findings
The extensive review of the literature yielded results that shed light on some crucial factors propelling branded functional beverages’ consumption behaviour in India, especially in the COVID-19 scenario.

Research limitations/implications
The study is based purely on a conceptual understanding of branded functional beverages’ consumption behaviour among Indian consumers. The conceptual framework proposed here results from the review of past literature. Therefore, the scientific world is invigorated to test further the implications presented in this study empirically.

Practical implications
The conceptual framework proposed here concerning consumer behaviour in functional beverages would guide the food marketers to segment and position their functional food offerings, mainly beverage variants, in the post-pandemic scenario. Moreover, the study findings enable policymakers to craft food policies to uphold the noble cause of moulding a healthy generation.

Originality/value
The COVID-19 pandemic has virtually shattered our food system and modified our associations with food in many different ways. The perception of health risks associated with COVID-19 might have influenced people’s food purchase and consumption behaviours. It is proposed that health-conscious people would prefer more health-promoting foods and beverages to strengthen their immunity. Several reports disclose that food consumers in India demand functional foods and beverages more extensively than before to include in their daily food basket. A detailed literature review revealed an evident shortage of research studies regarding consumer behaviour in functional beverages in India. No prior research works could be identified as to why consumers prefer functional beverages to benefit their overall health and immunity and what guides them to purchase or not to purchase such products, especially in the post-pandemic scenario. Therefore, this research work would be a primer that would guide future studies investigating consumers’ attitudes and purchasing behaviour of branded functional beverages.
An explorative study on the potential of green roofs providing thermal comfort conditions for indoor spaces in Kumasi, Ghana
Authors: Koranteng Christian , Nyame-Tawiah David , Gyimah Kwabena Abrokwa , Simons Barbara 
Published: September 2021

PurposeAs the global population keeps increasing with its associated urbanisation and climate change issues being experienced in various degrees worldwide, there is the need to find mitigating measures to improve thermal conditions within spaces. The study aimed to evaluate green roofs to determine whether they could provide thermal comfort within residential buildings.Design/methodology/approachForty-two-year weather data were retrieved from the Kumasi weather station to establish the pattern of the climatic variables. Furthermore, an experiment was conducted by constructing test cells to determine the potential of vegetation/green roofs on temperature development within spaces. This approach led to a simulation-based exploration of the thermal performance of the test cells to probe variables that could lead to the reduction in temperature after the models in the software (design-builder) had been validated.FindingsThe results on the 42 years (1976–2018) weather data showed a significant (p = 0.05) mean temperature increment of 2.0 °C. The constructed test cell with Setcreasea purpurea (Purple Heart) vegetation showed an annual mean temperature reduction of 0.4 °C (p = 0.05). In addition, the exploration using the simulation application showed combinations of various soil depth (70–500 mm) and leaf area indices (leaf area index of 2–5) having a potential to lower indoor temperature by 1.5 °C and its associated reduction in energy use. The option of green roofs as a valuable alternative to conventional roofs, given their potential in mitigating climate change, must be encouraged. A survey of occupants in six selected neighbourhoods in Kumasi showed varying subjective perceptions of several green issues (24–98%) and increases in temperature values because of the loss of greenery in the city.Originality/valueEmpirical data that point to the significant reduction of indoor temperature values and a subsequent reduction in energy use have been unearthed. Therefore, built environment professionals together with city authorities could invest in these sustainable measures to help humanity.
Glucagon‐like peptide‐1 receptor agonist treatment of high carbohydrate intake‐induced metabolic syndrome provides pleiotropic effects on cardiac dysfunction through alleviations in electrical and intracellular Ca2+ abnormalities and mitochondrial dysfunction
Authors: Durak Aysegul , Akkus Erman , Canpolat Asena Gokcay , Tuncay Erkan , Corapcioglu Demet , Turan Belma 
Published: September 2021

AbstractThe pleiotropic effects of glucagon‐like peptide‐1 receptor (GLP‐1R) agonists on the heart have been recognised in obese or diabetic patients. However, little is known regarding the molecular mechanisms of these agonists in cardioprotective actions under metabolic disturbances. We evaluated the effects of GLP‐1R agonist liraglutide treatment on left ventricular cardiomyocytes from high‐carbohydrate induced metabolic syndrome rats (MetS rats), characterised with insulin resistance and cardiac dysfunction with a long‐QT. Liraglutide (0.3 mg/kg for 4 weeks) treatment of MetS rats significantly reversed long‐QT, through a shortening the prolonged action potential duration and recovering inhibited K+‐currents. We also determined a significant recovery in the leaky sarcoplasmic reticulum (SR) and high cytosolic Ca2+‐level, which are confirmed with a full recovery in activated Na+/Ca2+‐exchanger currents (INCX). Moreover, the liraglutide treatment significantly reversed the depolarised mitochondrial membrane potential (MMP), increased production of oxidant markers, and cellular acidification together with the depressed ATP production. Our light microscopy analysis of isolated cardiomyocytes showed marked recoveries in the liraglutide‐treated MetS group such as marked reverses in highly dilated T‐tubules and SR‐mitochondria junctions. Moreover, we determined a significant increase in depressed GLUT4 protein level in liraglutide‐treated MetS group, possibly associated with recovery in casein kinase 2α. Overall, the study demonstrated a molecular mechanism of liraglutide‐induced cardioprotection in MetS rats, at most, via its pleiotropic effects, such as alleviation in the electrical abnormalities, Ca2+‐homeostasis, and mitochondrial dysfunction in ventricular cardiomyocytes.
Escaping the fate of Sisyphus: assessing resistome hybridization baits for antimicrobial resistance gene capture
Authors: Beaudry Megan S. , Thomas Jesse C. , Baptista Rodrigo P. , Sullivan Amanda H. , Norfolk William , Devault Alison , Enk Jacob , Kieran Troy J. , Rhodes Olin E. , Perry‐Dow K. Allison , Rose Laura J. , Bayona‐Vásquez Natalia J. , Oladeinde Adelumola , Lipp Erin K. , Sanchez Susan , Glenn Travis C. 
Published: September 2021

SummaryFinding, characterizing and monitoring reservoirs for antimicrobial resistance (AMR) is vital to protecting public health. Hybridization capture baits are an accurate, sensitive and cost‐effective technique used to enrich and characterize DNA sequences of interest, including antimicrobial resistance genes (ARGs), in complex environmental samples. We demonstrate the continued utility of a set of 19 933 hybridization capture baits designed from the Comprehensive Antibiotic Resistance Database (CARD)v1.1.2 and Pathogenicity Island Database (PAIDB)v2.0, targeting 3565 unique nucleotide sequences that confer resistance. We demonstrate the efficiency of our bait set on a custom‐made resistance mock community and complex environmental samples to increase the proportion of on‐target reads as much as >200‐fold. However, keeping pace with newly discovered ARGs poses a challenge when studying AMR, because novel ARGs are continually being identified and would not be included in bait sets designed prior to discovery. We provide imperative information on how our bait set performs against CARDv3.3.1, as well as a generalizable approach for deciding when and how to update hybridization capture bait sets. This research encapsulates the full life cycle of baits for hybridization capture of the resistome from design and validation (both in silico and in vitro) to utilization and forecasting updates and retirement.
Alphaproteobacteria facilitate Trichodesmium community trimethylamine utilization
Authors: Conover Asa E. , Morando Michael , Zhao Yiming , Semones Jacob , Hutchins David A. , Webb Eric A. 
Published: September 2021

SummaryIn the surface waters of the warm oligotrophic ocean, filaments and aggregated colonies of the nitrogen (N)‐fixing cyanobacterium Trichodesmium create microscale nutrient‐rich oases. These hotspots fuel primary productivity and harbour a diverse consortium of heterotrophs. Interactions with associated microbiota can affect the physiology of Trichodesmium, often in ways that have been predicted to support its growth. Recently, it was found that trimethylamine (TMA), a globally abundant organic N compound, inhibits N2 fixation in cultures of Trichodesmium without impairing growth rate, suggesting that Trichodesmium can use TMA as an alternate N source. In this study, 15N‐TMA DNA stable isotope probing (SIP) of a Trichodesmium enrichment was employed to further investigate TMA metabolism and determine whether TMA‐N is incorporated directly or secondarily via cross‐feeding facilitated by microbial associates. Herein, we identify two members of the marine Roseobacter clade (MRC) of Alphaproteobacteria as the likely metabolizers of TMA and provide genomic evidence that they converted TMA into a more readily available form of N, e.g., ammonium (NH4+), which was subsequently used by Trichodesmium and the rest of the community. The results implicate microbiome‐mediated carbon (C) and N transformations in modulating N2 fixation and thus highlight the involvement of host‐associated heterotrophs in global biogeochemical cycling.
Emotions and emotion work before, during and after HIV disclosure among Black gay and bisexual men living with HIV
Authors: Campbell Chadwick K. 
Published: September 2021

AbstractIn the United States, Black gay and bisexual men account for a quarter of HIV infections and face intersecting social and structural stigmas along the axes of race, sexuality and class. For those diagnosed with HIV, these inequities shape their lived experiences which include HIV disclosure. Public health has privileged HIV status disclosure as the appropriate moral and responsible choice to protect sex partners, reduce stigma and obtain social support. Though little is known about the emotional aspects of HIV disclosure for Black gay and bisexual men, or how they are shaped by social and structural contexts. Using the frameworks of healthism and emotion work, I explore HIV disclosure among a sample of 30 Black gay and bisexual men living with HIV in the Deep South. Drawing on in‐depth, qualitative interviews, I identify the emotion work that men engaged in to manage their own emotions and protect the emotions of others before, during and after disclosure or nondisclosure. These findings challenge public health research that has explored disclosure as discrete measurable events by illustrating how HIV disclosure is embedded in ongoing social and structural relations and provide insights that can guide new approaches focused on structural inequities that constitute HIV disclosure.
Planning versus the market: The dispute between Hayek and Mannheim and its contemporary relevance
Authors: Hammersley Martyn 
Published: September 2021

AbstractParallels have been drawn between the 1930s and today, notably the existence of unstable economic conditions as well as deepening, antagonistic ideological divisions. In the 1940s, two books appeared that presented opposing diagnoses of, and remedies for, the problems that faced Western societies at that time. In Man and Society, Karl Mannheim argued that forms of political organization had not adapted to changing social, economic, and technological conditions, and that this explained the rise of communism and fascism. He insisted that, in order to avoid disorder and political extremism, liberal democracies needed to engage in greater planning of their economic and social affairs, with sociology providing the synthesis of scientific knowledge required for this. Just a few years later, Friedrich Hayek published The Road to Serfdom, in which Mannheim's work was a central target. He insisted that only the preservation of liberal freedoms and competitive markets could prevent the spread of totalitarianism. This paper outlines the arguments of each of these authors and provides an account of some of the historical background against which their disagreement arose. It also explores the relevance of their very different positions today, at a time when the neoliberal ideology that Hayek championed continues to have great influence but is under increasing attack from across the political spectrum.
Metagenomics reveals contrasting energy utilization efficiencies of captive and wild camels (Camelus ferus)
Authors: YAN Liping , TANG Liping , ZHOU Zhichao , LU Wei , WANG Bo , SUN Zhicheng , JIANG Xue , HU Defu , LI Junqing , ZHANG Dong 
Published: September 2021

AbstractCaptive conditions can affect the symbiotic microbiome of animals. In this study, we compared the structural and functional differences of the gastrointestinal microbiomes of wild Bactrian camels (Camelus ferus) between wild and captive populations, as well as their different host energy utilization performances through metagenomics. The results showed that wild‐living camels harbored more microbial taxa related to the production of volatile fatty acids, fewer methanogens, and fewer genes encoding enzymes involved in methanogenesis, leading to higher energy utilization efficiency compared to that of captive‐living camels. These findings suggest that the wild‐living camel fecal microbiome demonstrates a series of adaptive characteristics that enable the host to adjust to a relatively barren field environment. Our study provides novel insights into the mechanisms of wildlife adaptations to habitats from the perspective of the microbiome.
Commensal microbiota and host metabolic divergence are associated with the adaptation of Diploderma vela to spatially heterogeneous environments
Authors: ZHU Wei , SHI Xiudong , QI Yin , WANG Xiaoyi , CHANG Liming , ZHAO Chunlin , ZHU Lifeng , JIANG Jianping 
Published: September 2021

AbstractHeterogeneous environment adaptation is critical to understand the species evolution and response to climate change. However, how narrow‐range species adapt to micro‐geographic heterogeneity has been overlooked, and there is a lack of insights from metabolism and commensal microbiota. Here, we studied the environmental adaptation for 3 geographic populations (>40 km apart) of Diploderma vela, a lizard endemic to dry‐hot valleys of the Hengduan Mountain Region. The climatic boundary caused a cooler, droughtier, and barren environment for northernmost population (RM) than the middle (QZK) and southernmost populations (FS). Correspondingly, significant divergences in liver and muscle metabolism and commensal microbiota were detected between RM and QZK or FS individuals, but not between QZK and FS individuals. Phospholipid composition, coenzyme level (i.e. pyridoxal and NAD+), and cholesterol metabolism (e.g. androgen and estriol synthesis) constituted the major metabolic difference between RM and QZK/FS groups. FS and QZK individuals kept abundant Proteobacteria and antifungal strains, while RM individuals maintained more Firmicutes and Bacteroidota. Strong associations existed between varied host metabolite and gut microbes. How were these interpopulation variations associated to the environment adaptation were discussed. These results provided some novel insights into the environmental adaptation and implicated the consequence of climate change on narrow‐range species.
Effect of infrared radiation on activity and conformation of polyphenol oxidase from Acetes chinensis
Authors: Zhang Jianyou , Zhou Guangcheng , Chen Lifan , Sun Lei , Fei Lifeng , Lyu Fei , Ding Yuting 
Published: September 2021

AbstractThe aim of this paper was to study the effect of infrared radiation (IR) on the activity and conformation of polyphenol oxidase (PPO) in Acetes chinensis. In this paper, the specific activity of PPO was increased from 21.2 to 643.4 U/mg by a four‐step purification. The results showed that IR treatment had greater effect on the enzyme activity and conformation of PPO than hot air (HA) treatment. After IR treatment at 70°C, the relative enzyme activity of PPO was 9.28%, the surface hydrophobicity index increased by 80.42%, and the content of sulfhydryl group decreased to 96.99% of the control group. The results of circular dichroism (CD) and Fourier transform infrared spectroscopy (FTIR) showed that the α‐helix of PPO treated by IR decreased and the random coil increased. The intrinsic fluorescence intensity of PPO decreased after IR treatment, indicating that the tertiary structure of PPO was destroyed. Scanning electron microscopy (SEM) results showed that the surface microstructure of PPO after IR treatment became clear and compact. In conclusion, IR treatment can completely destroy the secondary structure and tertiary structure of PPO and cause enzyme inactivation. This study provides a treatment for reducing the activity of PPO from A. chinensis during the production and processing.Practical ApplicationThis study shows that IR treatment has a better inhibitory effect on the activity of PPO than HA treatment. It provides a better treatment method for inactivating the activity of PPO from Acetes chinensis during the production and processing.
REST/NRSF deficiency impairs autophagy and leads to cellular senescence in neurons
Authors: Rocchi Anna , Carminati Emanuele , De Fusco Antonio , Kowalska Jagoda Aleksandra , Floss Thomas , Benfenati Fabio 
Published: September 2021

AbstractDuring aging, brain performances decline. Cellular senescence is one of the aging drivers and a key feature of a variety of human age‐related disorders. The transcriptional repressor RE1‐silencing transcription factor (REST) has been associated with aging and higher risk of neurodegenerative disorders. However, how REST contributes to the senescence program and functional impairment remains largely unknown. Here, we report that REST is essential to prevent the senescence phenotype in primary mouse neurons. REST deficiency causes failure of autophagy and loss of proteostasis, increased oxidative stress, and higher rate of cell death. Re‐establishment of autophagy reverses the main hallmarks of senescence. Our data indicate that REST has a protective role in physiological aging by regulating the autophagic flux and the senescence program in neurons, with implications for neurological disorders associated with aging.
Environmental conditions are more effective than nutrient availability and spatial processes on explaining microphytoplankton functional structure in a subtropical hypereutrophic reservoir
Authors: Alves Jonatas , Pinheiro‐Silva Lorena , Schuster Karling Fernanda , Matthiensen Alexandre , Petrucio Mauricio Mello 
Published: September 2021

AbstractThe structure of freshwater phytoplankton communities can be strongly influenced by environmental and spatial processes at different scales. To identify the main forces that drive phytoplankton structure becomes a relevant tool to understand several knowledge gaps in the phytoplankton ecology domain, such as community succession, biological invasions and responses to disturbances. We used the morphological‐based functional groups (MBFG) approach to investigate the influence of environmental conditions (physical and chemical factors), nutrient availability and spatial processes (habitat connection and species dispersal) in structuring microphytoplankton community in a subtropical hypereutrophic reservoir located in southern Brazil. Samples were taken quarterly, between February 2017 and December 2018. We used distance‐based multivariate analysis of variance (PERMANOVA) to evaluate potential spatial and seasonal dissimilarities in the MBFG’s biovolume, and variation partitioning approach (pRDA) to evaluate the role of environmental, nutrient availability and spatial components in explain the microphytoplankton community structure. We identified 16 microphytoplankton taxa, which were sorted into five MBFGs. Significant seasonal dissimilarities were observed in the MBFG’s biovolume. Higher relative biovolume of potential harmful cyanobacteria (MBFG III) and alien dinoflagellates (MBFG V) was observed in summer and autumn/winter, respectively. Pure environmental component (composed by physical and chemical predictors insolation, precipitation, total dissolved solids, turbidity, water temperature, water transparency and wind intensity) explained 45% of the variance in the MBFG’s biovolume, whereas pure nutrient availability component explained 7% and spatial component explained only 3% of the biotic variance. The results showed that the environmental factors – especially the seasonal‐influenced ones – are more effective than nutrient availability and spatial processes in explaining the microphytoplankton community structure in this ecosystem. Our findings alert to the need to consider the variability of the environmental conditions (mainly those directly influenced by seasonality) in phytoplankton community structure in disturbed freshwater ecosystems prone to eutrophication events.