bluuebunny/crossref_metadata_2025_split huggingface dataset:
Fruit fly surveillance in Togo (West Africa): state of diversity and prevalence of species
Authors: Amevoin Komina , Agboyi L. K. , Gomina M. , Kounoutchi K. , Bassimbako K. H. , Djatoite M. , Dawonou A. V. , Tagba A. 
Published: August 2021

AbstractThe production and marketing of fruits, especially mango, in sub-Saharan Africa are threatened by fruit flies (Diptera: Tephritidae). This baseline study analysed the biodiversity of fruit fly species, in mango orchards, in different ecological zones of Togo. Traps used to monitor the flies in the orchards consisted of dry baits, made from four types of parapheromones specific to the males of different species. Forty species of fruit flies were identified in the mango orchards in Togo. The most common species wereBactrocera dorsalis(Hendel),Ceratitis cosyra(Walker),Ceratitis fasciventris(Bezzi),Ceratitis capitata(Wiedemann),Ceratitis bremiiGuérin-Méneville,Dacus bivittatus(Bigot),Dacus humeralis(Bezzi),Dacus punctatifronsKarsch andZeugodacus cucurbitae(Coquillett). The invasiveB. dorsalisand the endogenous species,C. cosyrawere dominant in the mango producing areas of Togo because their prevalence were very high (B. dorsalis: 2.1 ≤ flies per trap per day (FTD) ≤ 472.2;C. cosyra: 0.34 ≤ FTD ≤ 97.28). There was no area free from fruit flies in Togo during the study. These results constitute an essential reference in the future evaluation of the effectiveness of any control activities initiated in Togo against fruit flies.
A Combination of Cervicovaginal Fluid Glutamate, Acetate and D-Lactate Identified Asymptomatic Low-Risk Women Destined to Deliver Preterm: a Prospective Cohort Study
Authors: Amabebe Emmanuel , Anumba Dilly O. C. 
Published: August 2021

AbstractDue to the modest predictive capacities and limited clinical application of transvaginal ultrasonographic cervical length (CL) and quantitative fetal fibronectin (qfFN) in pregnant women at low risk of preterm birth (PTB), we sought to determine the utility of cervicovaginal fluid (CVF) metabolites (by-products of host-microbial metabolism) for prediction of spontaneous PTB in asymptomatic low-risk women at mid-gestation. This was a prospective sub-cohort study from the ECCLIPPx study cohort. CVF from asymptomatic singleton women (20–22 weeks, n = 168) without a prior history of PTB were analysed for metabolites by enzyme-based spectrophotometry. CL, vaginal pH and qfFN were also measured. Correlation and predictive analyses were performed by Spearman’s correlation, and binary logistic regression and area under receiver operating characteristic curve (AUC), respectively. Of the 168 women enrolled, only CVF samples from 135 (80.4%) women were analysed. There were 6/135 (4.4%) spontaneous PTB (sPTBs), with two of these pregnancies ending ≤ 28 weeks’ gestation. Individually (AUC, 95% CI), only glutamate (0.72, 0.64–0.80) and CL (0.69, 0.60–0.77) were predictive of PTB. However, five multivariable models that more accurately predicted sPTB were also identified, i.e. a combination of: glutamate, acetate and D-lactate (GAD, 0.82, 0.74–0.89); CL and qfFN only (0.78, 0.70–0.85); CL, qfFN, glutamate and acetate (0.88, 0.81–0.93); CL, qfFN and GAD (0.94, 0.88–0.98); and GAD and pH (0.86, 0.79–0.92). Correlations between CL, pH and qfFN and metabolites were also observed. In this cohort, a midtrimester combination of CVF glutamate, acetate and D-lactate predicted preterm birth more accurately than individual metabolites, cervical length and fetal fibronectin with a very low false-positive rate and high positive predictive value. Further testing in populations with higher preterm birth rates is required.
Time heals all wounds: analysis of changes in temporal focus and implicit space–time mappings among survivors of the 2019 China earthquake over time
Authors: LI HENG 
Published: August 2021

abstractAccumulating evidence suggests that people’s sense of the spatial location of events in time is flexible across cultures, contexts, and individuals. Yet few studies have established whether time spatialization is correlated with traumatic experiences. Based on findings that people tend to demonstrate a past time orientation when suffering from disasters, the present research investigated how earthquake experience is associated with temporal focus and time spatialization. Study 1 compared responses of residents in an earthquake-hit area with those of residents in a non-disaster area about two weeks after the disaster had occurred. The results showed that participants in the disaster area were more past-focused and produced more past-in-front responses than participants in the non-disaster area. In Study 2, a follow-up survey was conducted in the same areas ten months after the earthquake to examine whether the impact of disasters on spatial conceptions of time would decay as time elapsed. The findings indicated that participants in these two areas showed no differences in temporal focus and implicit space–time mappings. Taken together, these findings provide support for the Temporal Focus Hypothesis. They also have implications for understanding fluctuation in temporal focus and the high malleability of temporal mappings across individuals.
Dietary and serum n-3 PUFA and polycystic ovary syndrome: a matched case–control study
Authors: Lu Ling , Li Xiaoqin , Lv Lin , Xu Yao , Wu Baohua , Huang Chaolin 
Published: August 2021

AbstractThe results of epidemiological studies involving n-3 PUFA and polycystic ovary syndrome (PCOS) are scarce. This matched case–control study assessed the associations between n-3 PUFA and PCOS prevalence in 325 pairs of PCOS cases and healthy controls. Dietary information was assessed using a 102-item FFQ. Fatty acids in serum phospholipids were measured with a GC method. We found that n-3 PUFA in serum phospholipids were inversely associated with PCOS prevalence, including total, long-chain and individual PUFA (e.g. docosapentaenoic acid (DPA), EPA and DHA). Compared with the lowest tertile (T1), the adjusted OR and their 95% CI for the highest tertile (T3) were 0·63 (0·40, 0·93) for total n-3 PUFA, 0·60 (0·38, 0·92) for long-chain n-3 PUFA, 0·68 (0·45, 1·01) for DHA, 0·70 (0·45, 1·05) for EPA and 0·72 (0·45, 1·08) for DPA. For dietary intake of n-3 PUFA, significant inverse associations were found only for long-chain n-3 PUFA (Ptrend = 0·001), EPA (Ptrend = 0·047) and DHA (Ptrend = 0·030). Both dietary and serum n-3 PUFA, mainly EPA and DPA, were negatively correlated with PCOS-related parameters, such as BMI, fasting insulin, total testosterone and high-sensitivity C-reactive protein, but positively correlated with follicle-stimulating hormone and sex hormone-binding globulin. These results indicated inverse associations between n-3 PUFA, especially long-chain n-3 PUFA, and PCOS prevalence. Higher intakes of n-3 PUFA might be considered a protective factor for PCOS among Chinese females.
Posets pluton: a geochronological piece in the puzzle of the Axial Zone of the Pyrenees
Authors: Esteban JJ , Cuevas J , Tubía JM , Hilario A , Larionov A , Sergeev S 
Published: August 2021

AbstractA detailed geochronological study was conducted on zircons from a diorite sample of the Posets pluton (Axial Zone, Pyrenees). The extracted igneous zircons constrain the emplacement of the pluton to 302 ± 2 Ma and 301 ± 3 Ma, by means of U–Pb sensitive high-resolution ion microprobe (SHRIMP) and laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) analyses, respectively. Considering the syn- to late-tectonic emplacement of the Posets pluton during the main Variscan deformation event (D2), the obtained ages constrain the long-lasting D2, associated with the dextral transpression registered through the Axial Zone of the Pyrenees.
Going further post-RNA-seq:In silicofunctional analyses revealing candidate genes and regulatory elements related to mastitis in dairy cattle
Authors: Passe Pereira Hyago , Lima Verardo Lucas , Morena Del Cambre Amaral Weller Mayara , Paula Sbardella Ana , Prado Munari Danísio , Morais de Paiva Daibert Raquel , Araújo Carvalho Wanessa , Antonio Machado Marco , Fonseca Martins Marta 
Published: August 2021

AbstractThis study aimed to obtain a better understanding of the regulatory genes and molecules involved in the development of mastitis. For this purpose, the transcription factors (TF) and MicroRNAs (miRNA) related to differentially expressed genes previously found in extracorporeal udders infected withStreptococcus agalactiaewere investigated. The Gene-TF network highlightedLOC515333,SAA3,CD14,NFKBIA,APOC2andLOC100335608and genes that encode the most representative transcription factorsSTAT3,PPARG,EGR1andNFKB1for infected udders. In addition, it was possible to highlight, through the analysis of the gene-miRNA network, genes that could be post-transcriptionally regulated by miRNAs, such as the relationship between theCCL5gene and the miRNA bta-miR-363. Overall, our data demonstrated genes and regulatory elements (TF and miRNA) that can play an important role in mastitis resistance. The results provide new insights into the first functional pathways and the network of genes that orchestrate the innate immune responses to infection byStreptococcus agalactiae. Our results will increase the general knowledge about the gene networks, transcription factors and miRNAs involved in fighting intramammary infection and maintaining tissue during infection and thus enable a better understanding of the pathophysiology of mastitis.
Theorising sexual violence in global politics: Improvising with feminist theory
Authors: Zalewski Marysia 
Published: August 2021

AbstractA key curiosity animating this article concerns how sexual violence is theorised. The work of feminist scholars has been crucial in unearthing ways in which women's traditionally demeaned bodies regularly materialised as ‘easy targets’ for such violence. The gift of the concept of gender has played a significant role in facilitating the production of this corpus of knowledge. Less noticed in the literature, in policy and legislation has been sexual violence against men – an egregious omission. Yet it seems that redeploying the concept of gender to make sense of sexual violence against men and elevate this violence into the realms of theoretical and legislative attention is not straightforward. Identifying feminist work as in part responsible for the rendering of sexual violence against men as too ‘unseen’ in theory provoked my attention, though it's not that I place feminist theory as ‘innocent’ or infallible – far from it. In this article I unpack some of the complexities around theorising sexual politics in Global Politics turning towards the aesthetics of feminist thinking to help reconsider the way connections take shape between gender, sex and violence. Underpinning this discussion are questions about feminist intentions to transform patriarchal and colonial structures and institutions.
Facilitating Age-Conscious Student Development through Lecture-Based Courses on Aging
Authors: Russell Elizabeth , Thériault Éric R. , Colibaba Amber 
Published: August 2021

AbstractAgeism is pervasive and socially normalized, and population aging has created a need to understand how views of aging and of older people, typically considered to be people over the age of 65, can be improved. This study sought to understand how undergraduate students’ attitudes towards older adults and the aging process may be influenced after completing a typical, lecture-based undergraduate course on aging that lacked service-learning components. Two undergraduate student cohorts (n= 40) at two Canadian universities participated in semi-structured focus groups/interviews, describing how the course may have impacted their perceptions of the aging process and of older adults. An iterative collaborative qualitative analysis demonstrated that course content stimulated a deeper understanding of the aging process, prompting a reduction in and increased awareness of ageism, and enhanced personal connection with aging, ultimately facilitating the development of an age-conscious student. Lecture-based courses focused on aging may be sufficient to facilitate positive attitude change among undergraduate students towards older adults and the aging process.
Indeterminate and oncogenic potential: CHIP vs CHOP mutations in AML with NPM1 alteration
Authors: Cappelli Luca Vincenzo , Meggendorfer Manja , Baer Constance , Nadarajah Niroshan , Hutter Stephan , Jeromin Sabine , Dicker Frank , Kern Wolfgang , Haferlach Torsten , Haferlach Claudia , Höllein Alexander 
Published: August 2021

AbstractIn AML patients, recurrent mutations were shown to persist in remission, however, only some have a prognostic value and persistent mutations might therefore reflect a re-established premalignant state or truly active disease causing relapse. We aimed to dissect the nature of co-mutations in NPM1 mutated AML where the detection of NPM1 transcripts allows highly specific and sensitive detection of complete molecular remission (CMR). We analysed 150 consecutive patients who achieved CMR following intensive treatment by next generation sequencing on paired samples at diagnosis, CMR and relapse (38/150 patients). Patients with persistence or the acquisition of non-DTA (DNMT3A, TET2, ASXL1) mutations at CMR (23/150 patients, 15%) have a significantly worse prognosis (EFS HR = 2.7, p = 0.003; OS HR = 3.6, p = 0.012). Based on clonal evolution analysis of diagnostic, CMR and relapse samples, we redefine pre-malignant mutations and include IDH1, IDH2 and SRSF2 with the DTA genes in this newly defined group. Only the persistence or acquisition of CHOP-like (clonal hematopoiesis of oncogenic potential) mutations was significantly associated with an inferior outcome (EFS HR = 4.5, p = 0.0002; OS HR = 5.5, p = 0.002). Moreover, the detection of CHOP-like mutations at relapse was detrimental (HR = 4.5, p = 0.01). We confirmed these findings in a second independent whole genome sequencing cohort.
BCLXL gene therapy moderates neuropathology in the DBA/2J mouse model of inherited glaucoma
Authors: Donahue Ryan J. , Fehrman Rachel L. , Gustafson Jenna R. , Nickells Robert W. 
Published: August 2021

AbstractAxonal degeneration of retinal ganglion cells (RGCs) causes blindness in glaucoma. Currently, there are no therapies that target axons to prevent them from degenerating. Activation of the BAX protein has been shown to be the determining step in the intrinsic apoptotic pathway that causes RGCs to die in glaucoma. A putative role for BAX in axonal degeneration is less well elucidated. BCLXL (BCL2L1) is the primary antagonist of BAX in RGCs. We developed a mCherry-BCLXL fusion protein, which prevented BAX recruitment and activation to the mitochondria in tissue culture cells exposed to staurosporine. This fusion protein was then packaged into adeno-associated virus serotype 2, which was used to transduce RGCs after intravitreal injection and force its overexpression. Transduced RGCs express mCherry-BCLXL throughout their somas and axons along the entire optic tract. In a model of acute optic nerve crush, the transgene prevented the recruitment of a GFP-BAX fusion protein to mitochondria and provided long-term somal protection up to 12 weeks post injury. To test the efficacy in glaucoma, DBA/2J mice were transduced at 5 months of age, just prior to the time they begin to exhibit ocular hypertension. Gene therapy with mCherry-BCLXL did not affect the longitudinal history of intraocular pressure elevation compared to naive mice but did robustly attenuate both RGC soma pathology and axonal degeneration in the optic nerve at both 10.5 and 12 months of age. BCLXL gene therapy is a promising candidate for glaucoma therapy.
The transcription factor CmLEC1 positively regulates the seed-setting rate in hybridization breeding of chrysanthemum
Authors: Xu Sujuan , Wu Ze , Hou Huizhong , Zhao Jingya , Zhang Fengjiao , Teng Renda , Ding Liping , Chen Fadi , Teng Nianjun 
Published: August 2021

Abstract
Distant hybridization is widely used to develop crop cultivars, whereas the hybridization process of embryo abortion often severely reduces the sought-after breeding effect. The LEAFY COTYLEDON1 (LEC1) gene has been extensively investigated as a central regulator of seed development, but it is far less studied in crop hybridization breeding. Here we investigated the function and regulation mechanism of CmLEC1 from Chrysanthemum morifolium during its seed development in chrysanthemum hybridization. CmLEC1 encodes a nucleic protein and is specifically expressed in embryos. CmLEC1’s overexpression significantly promoted the seed-setting rate of the cross, while the rate was significantly decreased in the amiR-CmLEC1 transgenic chrysanthemum. The RNA-Seq analysis of the developing hybrid embryos revealed that regulatory genes involved in seed development, namely, CmLEA (late embryogenesis abundant protein), CmOLE (oleosin), CmSSP (seed storage protein), and CmEM (embryonic protein), were upregulated in the OE (overexpressing) lines but downregulated in the amiR lines vs. wild-type lines. Future analysis demonstrated that CmLEC1 directly activated CmLEA expression and interacted with CmC3H, and this CmLEC1–CmC3H interaction could enhance the transactivation ability of CmLEC1 for the expression of CmLEA. Further, CmLEC1 was able to induce several other key genes related to embryo development. Taken together, our results show that CmLEC1 plays a positive role in the hybrid embryo development of chrysanthemum plants, which might involve activating CmLEA’s expression and interacting with CmC3H. This may be a new pathway in the LEC1 regulatory network to promote seed development, one perhaps leading to a novel strategy to not only overcome embryo abortion during crop breeding but also increase the seed yield.
Bisection of the X chromosome disrupts the initiation of chromosome silencing during meiosis in Caenorhabditis elegans
Authors: Rappaport Yisrael , Achache Hanna , Falk Roni , Murik Omer , Ram Oren , Tzur Yonatan B. 
Published: August 2021

AbstractDuring meiosis, gene expression is silenced in aberrantly unsynapsed chromatin and in heterogametic sex chromosomes. Initiation of sex chromosome silencing is disrupted in meiocytes with sex chromosome-autosome translocations. To determine whether this is due to aberrant synapsis or loss of continuity of sex chromosomes, we engineered Caenorhabditis elegans nematodes with non-translocated, bisected X chromosomes. In early meiocytes of mutant males and hermaphrodites, X segments are enriched with euchromatin assembly markers and active RNA polymerase II staining, indicating active transcription. Analysis of RNA-seq data showed that genes from the X chromosome are upregulated in gonads of mutant worms. Contrary to previous models, which predicted that any unsynapsed chromatin is silenced during meiosis, our data indicate that unsynapsed X segments are transcribed. Therefore, our results suggest that sex chromosome chromatin has a unique character that facilitates its meiotic expression when its continuity is lost, regardless of whether or not it is synapsed.
Nanopore sequencing of brain-derived full-length circRNAs reveals circRNA-specific exon usage, intron retention and microexons
Authors: Rahimi Karim , Venø Morten T. , Dupont Daniel M. , Kjems Jørgen 
Published: August 2021

AbstractCircular RNA (circRNA) is a class of covalently joined non-coding RNAs with functional roles in a wide variety of cellular processes. Their composition shows extensive overlap with exons found in linear mRNAs making it difficult to delineate their composition using short-read RNA sequencing, particularly for long and multi-exonic circRNAs. Here, we use long-read nanopore sequencing of nicked circRNAs (circNick-LRS) and characterize a total of 18,266 and 39,623 circRNAs in human and mouse brain, respectively. We further develop an approach for targeted long-read sequencing of a panel of circRNAs (circPanel-LRS), eliminating the need for prior circRNA enrichment and find >30 circRNA isoforms on average per targeted locus. Our data show that circRNAs exhibit a large number of splicing events such as novel exons, intron retention and microexons that preferentially occur in circRNAs. We propose that altered exon usage in circRNAs may reflect resistance to nonsense-mediated decay in the absence of translation.
Adipose tissue hyaluronan production improves systemic glucose homeostasis and primes adipocytes for CL 316,243-stimulated lipolysis
Authors: Zhu Yi , Li Na , Huang Mingyang , Bartels Mason , Dogné Sophie , Zhao Shangang , Chen Xi , Crewe Clair , Straub Leon , Vishvanath Lavanya , Zhang Zhuzhen , Shao Mengle , Yang Yongjie , Gliniak Christy M. , Gordillo Ruth , Smith Gordon I. , Holland William L. , Gupta Rana K. , Dong Bingning , Caron Nathalie , Xu Yong , Akgul Yucel , Klein Samuel , Scherer Philipp E. 
Published: August 2021

AbstractPlasma hyaluronan (HA) increases systemically in type 2 diabetes (T2D) and the HA synthesis inhibitor, 4-Methylumbelliferone, has been proposed to treat the disease. However, HA is also implicated in normal physiology. Therefore, we generated a Hyaluronan Synthase 2 transgenic mouse line, driven by a tet-response element promoter to understand the role of HA in systemic metabolism. To our surprise, adipocyte-specific overproduction of HA leads to smaller adipocytes and protects mice from high-fat-high-sucrose-diet-induced obesity and glucose intolerance. Adipocytes also have more free glycerol that can be released upon beta3 adrenergic stimulation. Improvements in glucose tolerance were not linked to increased plasma HA. Instead, an HA-driven systemic substrate redistribution and adipose tissue-liver crosstalk contributes to the systemic glucose improvements. In summary, we demonstrate an unexpected improvement in glucose metabolism as a consequence of HA overproduction in adipose tissue, which argues against the use of systemic HA synthesis inhibitors to treat obesity and T2D.
Manipulating electron redistribution to achieve electronic pyroelectricity in molecular [FeCo] crystals
Authors: Sadhukhan Pritam , Wu Shu-Qi , Long Jeremy Ian , Nakanishi Takumi , Kanegawa Shinji , Gao Kaige , Yamamoto Kaoru , Okajima Hajime , Sakamoto Akira , Baker Michael L. , Kroll Thomas , Sokaras Dimosthenis , Okazawa Atsushi , Kojima Norimichi , Shiota Yoshihito , Yoshizawa Kazunari , Sato Osamu 
Published: August 2021

AbstractPyroelectricity plays a crucial role in modern sensors and energy conversion devices. However, obtaining materials with large and nearly constant pyroelectric coefficients over a wide temperature range for practical uses remains a formidable challenge. Attempting to discover a solution to this obstacle, we combined molecular design of labile electronic structure with the crystal engineering of the molecular orientation in lattice. This combination results in electronic pyroelectricity of purely molecular origin. Here, we report a polar crystal of an [FeCo] dinuclear complex exhibiting a peculiar pyroelectric behavior (a substantial sharp pyroelectric current peak and an unusual continuous pyroelectric current at higher temperatures) which is caused by a combination of Fe spin crossover (SCO) and electron transfer between the high-spin Fe ion and redox-active ligand, namely valence tautomerism (VT). As a result, temperature dependence of the pyroelectric behavior reported here is opposite from conventional ferroelectrics and originates from a transition between three distinct electronic structures. The obtained pyroelectric coefficient is comparable to that of polyvinylidene difluoride at room temperature.