bluuebunny/crossref_metadata_2025_split huggingface dataset:
Clinical, immunophenotypic, and cytogenetic characteristics of high‐grade myelodysplastic syndromes with CD41‐positive progenitor cells
Authors: Ogata Kiyoyuki , Sei Kazuma , Kawahara Naoya , Ogata Mika , Yamamoto Yumi 
Published: December 2021

AbstractBackground: Patients with myelodysplastic syndromes (MDS) with progenitors expressing CD41 (CD41+ MDS) showed a poor prognosis in a previous study but their detailed characteristics remain unclear.Methods: One hundred thirty‐seven subjects at our institution were diagnosed with excess blasts (EB)‐1, EB‐2, and acute myeloid leukemia with a low blast count (20%–30%). The immunophenotypes of progenitor cells in their bone marrow (BM) were determined by CD45‐gating flow cytometry. A false‐positive reaction to CD41 was eliminated by examining the flow cytometry data of lymphocytes and monocytes in addition to progenitors and by examining CD42b in histological sections. The characteristics were compared between CD41+ and CD41− MDS patients.Results: Forty‐three patients (31%) were CD41+. Additionally, 91% of the CD41+ MDS patients were very high‐risk defined by the Revised International Prognostic Score System, which was higher than in patients with CD41− MDS (p = 0.015). Approximately 60% of the CD41+ MDS patients had a monosomal karyotype and very poor cytogenetics, which was higher than in CD41− MDS patients (p < 0.001). Normal cytogenetics was less common in CD41+ patients (p = 0.0016). Blasts with bleb formation were more abundant in CD41+ MDS patients (p = 0.026). All CD41+ MDS patients were positive for CD13 and were mostly positive for CD33. The frequency of aberrant expression of other antigens on progenitors was similar between CD41+ and CD41− MDS patients.Conclusions: We determined clinical, immunophenotypic, and cytogenetic characteristics of CD41+ MDS patients. Further studies are needed to improve the survival of these patients.
A golden eagle optimized hybrid multilayer perceptron convolutional neural network architecture‐based three‐stage mechanism for multiuser cognitive radio network
Authors: Justus J. Jean , Anuradha M. 
Published: December 2021

SummaryCognitive radio (CR) was developed to solve the issue of spectrum scarcity because CR users are unlicensed and can use the underutilized licensed spectrum without interfering with licensed users. During handoff, however, this CR creates a target channel sequence (TCS) to transfer the channel to the multiusers. It must also deal with issues such as equally assigning TCS to different secondary users (SUs), channel access collisions, and channel obsolesces during TCS development. TCS is issued to SUs in the same and different primary user (PU) areas. In this paper, the hybrid multilayer perceptron (MLP)–convolutional neural network (CNN) (MLP‐CNN) technique is specifically proposed to provide services to the SU even under proactive TCS constraints, as well as to address the aforementioned challenges. The MLP can increase cell quality by resolving the transmission time gap problem that occurs during the proactive stage. The MLP determines the characteristics of each SU in a specific area with high reliability for channel allocation, while the CNN allocates the channel based on the characteristics of the entire SU present in a specific area. This hybrid MLP‐CNN approach, on the other hand, is unable to achieve the optimal degree of optimized TCS generation accuracy due to the need for parameter fine‐tuning, which is accomplished using the golden eagle optimization technique. Different parameters, such as single‐user scenario, multiuser scenario, multichannel scenario, and network access overhead, are used to measure the performance of the proposed model. The handoff delay, average interval per target channel, and the overhead that occurs during the transmission of data by our proposed method are 0.56%, 0.17%–0.03%, and 50%–10% respectively. Thus, our proposed method outperformance all the existing approaches.
Impacts of land use and landscape pattern on water quality at multiple spatial scales in a subtropical large river
Authors: Shu Xiao , Wang Weibo , Zhu Mingyong , Xu Jilei , Tan Xiang , Zhang Quanfa 
Published: December 2021

AbstractThe coupling between land use/landscape pattern and water quality in river systems varies across different spatial and temporal scales. It is important to understand the association between water quality and land use/landscape pattern across different spatial and temporal scales for the protection of water resources. Here, we measured seasonal water quality at 12 sub‐basins in the upper reaches of the Han River (UHR) between 2010 and 2018. We conducted factor analysis and redundancy analysis to determine the links between land use and water quality at multiple spatial scales and to identify the main factors influencing water quality. We found that the concentration of nutrients, including total nitrogen, total phosphorus, nitrate‐N and ammonium‐N, was higher during the wet season than the dry season. Total nitrogen was the main driver of nutrient pollution in the UHR, whereas total phosphorus was identified as another potential nutrient pollutant. We also found that water quality parameters were more strongly related to land use types during the wet season than the dry season. Croplands and urban lands increased phosphorus concentrations of river water, whereas forest and grasslands decreased the nitrogen concentrations of river water at the sub‐basin scale. Land use at the riparian zone scales better explained variations in water quality than land use at sub‐basin scales. The explained variations in landscape metrics were generally higher during the dry season than the wet season. The largest patch index and Shannon's diversity index were the main predictors of river water quality in the UHR.
Impact of intravenous ferric carboxymaltose on heart failure with preserved and reduced ejection fraction
Authors: López‐Vilella Raquel , Lozano‐Edo Silvia , Arenas Martín Patricia , Jover‐Pastor Pablo , Ezzitouny Meryem , Sorolla Romero José , Calvo Asensio María , Martínez‐Solé Julia , Guerrero Cervera Borja , Sánchez Martínez José Carlos , Donoso Trenado Víctor , Sánchez‐Lázaro Ignacio , Martinez Dolz Luis , Almenar Bonet Luis 
Published: December 2021

AbstractAimsHeart failure (HF) is a proinflammatory disease often associated with the onset of iron deficiency (ID). ID alters mitochondrial function, reducing the generation of cellular energy in skeletal muscle and cardiomyocytes. This study aimed to analyse the response of patients with HF to intravenous iron administration according to the type of HF: preserved ejection fraction (HFpEF) or reduced ejection fraction (HFrEF).Methods and resultsWe conducted a retrospective, single‐centre study of 565 consecutive outpatients diagnosed with HF, recruited over 5 years, who were given intravenous ferric carboxymaltose (FCM) for the treatment of ID [defined as ferritin < 100 μg/L or ferritin 100–300 μg/L with transferrin saturation (TSAT) < 20%]. Clinical, laboratory, and echocardiographic parameters were analysed before and after administration. After FCM administration, overall ferritin, TSAT, and haemoglobin levels increased up to 5‐fold, 1.6‐fold, and 1.1‐fold, respectively, relative to baseline values in HF patients with reduced and preserved ejection fraction (P < 0.0001), with a greater increase in ferritin and TSAT in HFpEF patients. The left ventricular ejection fraction of the overall series improved by 8 percentage points in both types of HF (from 40% to 48%, P < 0.0001). The percentage of patients with normalization of right ventricular function increased by 6.9 points (from 74.1% to 81%) in HFpEF patients and by 6.4 points (from 53% to 59.4%) in the HFrEF subgroup (P < 0.0001). New York Heart Association functional status slightly improved, from a median of 2.4 (interquartile range, IQR: 2–2.7) to 1.9 (IQR: 1.5–2.5; P < 0.0001) after FCM in both types of HF. No changes were noted in plasma levels of liver enzymes, creatinine, or natriuretic peptide (P > 0.05).ConclusionsIntravenous iron administration appeared to improve ejection fraction and cardiac functional status in outpatients with ID and HF with both preserved and reduced ejection fraction.
Inflammatory cardiomyopathy of possibly overlapping aetiology: a case posing treatment dilemma and potential association
Authors: Nakagama Shun , Candray Katherine , Yamamoto Tasuku , Tsugeno Yuta , Nakagama Yu , Kido Yasutoshi , Nitahara Yuko , Maejima Yasuhiro , Sasano Tetsuo 
Published: December 2021

AbstractWe report on a 52‐year‐old Brazilian immigrant woman with past histories of chronic kidney disease and uveitis, presenting with symptomatic atrioventricular block. Her country of origin being endemic for Trypanosoma cruzi infection, we suspected Chagas disease as the aetiology, diagnosis of which was confirmed by serological tests. Further systemic workup identified an emerging nodular lesion in the lung, which turned out to be a sarcoid epithelioid granuloma on biopsy. Involvement of the kidneys and eyes was suggestive of systemic extension of the lung sarcoidosis. Although imaging modalities did not detect inflammatory foci in the myocardium, the rare coexistence of histologically proven sarcoidosis raised the intriguing concept of cardiac manifestation having arisen from two possibly overlapping aetiologies: Chagas disease and cardiac sarcoidosis. The case highlights a treatment dilemma increasingly likely to be encountered in this globalized world, and also raises the potential, but intriguing, association of these two diseases.
Grafting from Fluoropolymers Using ATRP: What is Missing?
Authors: Guerre Marc , Semsarilar Mona , Ladmiral Vincent 
Published: December 2021

AbstractGrafting polymers from fluoropolymer backbones is very advantageous to modify the properties of these often non‐functional materials. It is particularly useful in the field of ultrafiltration membranes to tune the hydrophilicity of PVDF membranes and to add other properties such as antifouling for example. One of the main techniques used to effect such grafting is ATRP. A large number of papers are published every year on this topic. However, careful examination of this body of work reveals that the evidence provided to support the covalent bonding of polymer chains on the fluoropolymers are often limited and rather unconvincing. This is of course due to the difficult detection of the rare connecting covalent bonds. Nevertheless, this detection problem is not the only issue. Grafting from fluoropolymers using ATRP relies on the homolytic cleavage of C−F bond, notorious for their high stability and BDE. Moreover, fluoropolymers based on VDF are prone to dehydrofluorination by reaction with bases and nucleophiles, which leads to the formation of unsaturations within the polymer backbone. This reaction is often completely ignored even though ATRP ligands can trigger this elimination. These two very often overlooked reactivity features seriously question the use of ATRP as an efficient grafting technique for fluoropolymers.
Cathodic corrosion of Au in aqueous methanolic alkali metal hydroxide electrolytes: Notable role of water
Authors: Elnagar Mohamed M. , Jacob Timo , Kibler Ludwig A. 
Published: December 2021

AbstractCathodic corrosion is an electrochemical process that induces restructuring, roughening, and etching of metal surfaces at a highly negative surface charge density, yet, details of the reaction mechanism are not fully resolved. An in‐depth fundamental understanding of the processes and parameters underlying cathodic corrosion is crucial for tailoring the surface structure of the metal electrodes and for synthesizing shape‐ and size‐controlled nanoparticles. Here, we investigate the relevance of water and hydrogen evolution in the cathodic corrosion process. To achieve this aim, Au electrodes were polarized at ‐1.6 V versus RHE in KOH and NaOH electrolytes prepared using different water + methanol mixtures. Structural changes of the Au surfaces were studied by cyclic voltammetry and monitored by scanning electron microscopy (SEM). Most importantly, cathodic corrosion does not take place in the absence of water. There is no detectable bubble formation due to the hydrogen evolution reaction on Au in purely methanolic alkali. Furthermore, the electrochemically active surface area, facet distribution, and surface morphology of Au electrodes are significantly altered upon cathodic polarization as a function of the water concentration. Cathodic corrosion features become more and more pronounced with a further increase in water content. In addition, substantial differences in the surface structure of Au are observed as a function of the nature and concentration of alkali metal cations. Overall, this study provides a more detailed understanding of the role of water and the hydrogen evolution reaction in dominating cathodic corrosion, which might advance the understanding of this phenomenon.
Combination of quantitative trait locus (QTL) mapping and transcriptome analysis reveals submerged germination QTLs and candidate genes controlling coleoptile length in rice
Authors: Kong Weilong , Li Shuangmiao , Zhang Chenhao , Qiang Yalin , Li Yangsheng 
Published: December 2021

AbstractSubmerged germination ability is a key trait of rice (Oryza sativa L.) in the success of water direct seeding. Therefore, mining submerged germination‐related loci and seeking high tolerant rice germplasms are important for developing rice direct seeding breeding strategies. Here, we treated 272 recombinant inbred lines (RILs, F15) from a cross from Luohui 9 (indica) and RPY geng (japonica) with simulated submergence for 7 days. We successfully bred several superior homozygous RILs with high submergence resistance, namely, R53, R137, R148, R176, and R180, via indica and japonica hybridization strategies. Then, the QTL mapping of coleoptile length trait was performed by R/qtl and IciMapping softwares based on the high‐density bin marker genetic map and two QTLs responsible for coleoptile length were detected in chromosomes 1 (qCL‐1.1) and 3 (qCL‐3.1). The haplotype results of RILs showed that the aggregation of elite alleles of these two QTLs in an individual was beneficial to improving the rice submergence tolerance. According to contrasting genotype of qCL‐1.1 and qCL‐3.1 with different coleoptile lengths, RIL07 (R07) and R180 were selected to further RNA‐seq analysis for analyzing the possible molecular mechanisms of rice under submergence stress. Finally, LOC_Os01g04430, LOC_Os01g04530, and LOC_Os03g22720 for qCL‐1.1 and qCL‐3.1 were characterized to affect submergence stress by the overlapped analysis of two independent sets of RNA‐seq data, qRT‐PCR verification, and gene sequence alignments. In this study, the submerged germination‐related QTLs/genes and high submergence‐tolerant RILs provided new genetic resources for breeding rice varieties suitable for direct seeding via molecular breeding strategies in the immediate future.
Compromised fractalkine signaling delays microglial occupancy of emerging modules in the multisensory midbrain
Authors: Brett Cooper A. , Carroll Julianne B. , Gabriele Mark L. 
Published: December 2021

AbstractMicroglial cells (MGCs) are highly dynamic and have been implicated in shaping discrete neural maps in several unimodal systems. MGCs respond to numerous cues in their microenvironment, including the neuronally expressed chemokine, fractalkine (CX3CL1), via interactions with its corresponding fractalkine receptor (CX3CR1). The present study examines microglial and CX3CL1 patterns with regard to the emerging modular‐extramodular matrix organization within the lateral cortex of the inferior colliculus (LCIC). The LCIC is a multisensory shell region of the midbrain inferior colliculus where discrete compartments receive modality‐specific connections. Somatosensory inputs terminate within modular confines, while auditory inputs target the surrounding matrix. Glutamic acid decarboxylase (GAD) is an established marker of LCIC modules in developing mouse. During early postnatal development, multimodal LCIC afferents segregate into discrete, neurochemically defined compartments. Here, we analyzed neonatal GAD67‐GFP (GFP is defined as green fluorescent protein) and CX3CR1‐GFP mice to assess: (1) whether MGCs are recruited to distinct LCIC compartments known to be undergoing active circuit assembly, and (2) if such behaviors are fractalkine signaling‐dependent. MGCs colonize the nascent LCIC by birth and increase in density until postnatal day 12 (P12). At the peak critical period (P4‐P8), MGCs conspicuously border emerging LCIC modules, prior to their subsequent invasion by P12. CX3CL1 expression becomes distinctly modular at P12, in keeping with the notion of fractalkine‐mediated recruitment of microglia to modular centers. In CX3CR1GFP/GFP mice with compromised fractalkine signaling, microglial recruitment into modules is delayed. Taken together, these results suggest a potential role for microglia and fractalkine signaling in sculpting multisensory LCIC maps during an early critical period.
Prognostic factors in mammary analogue secretory carcinomas of the parotid gland: Systematic review and meta‐analysis
Authors: Janik Stefan , Faisal Muhammad , Marijić Blazen , Grasl Stefan , Grasl Matthaeus Ch. , Heiduschka Gregor , Erovic Boban M. 
Published: December 2021

AbstractMammary analogue secretory carcinomas (MASCs) of the parotid gland are considered as low‐grade malignancies with good clinical outcome but lacking data regarding prognostic factors. We performed meta‐analysis assessing prognostic factors for disease‐free survival (DFS) and overall survival (OS) in 256 patients with MASCs of the parotid gland. A total of 73 studies have met the inclusion criteria and 76.3% of patients were seen with T1 and T2 tumors and negative neck nodes. Lymph node metastasis (57.4%) and distant recurrences (46.2%) were particularly found in T4 tumors (p < 0.001). DFS at 5 and 10 years was 77.9% and 47.2% compared to 88.1% and 77.2% for OS at the same time points. Male sex, T3‐T4 tumors, and recurrent disease represented independent worse prognosticators for survival outcome. Altogether, parotid gland MASCs show good long‐term outcome, but T4 tumors behave significantly more aggressive and require extended treatment strategies along with close follow‐ups.
Gradual decorrelation of CA3 ensembles associated with contextual discrimination learning is impaired by Kv1.2 insufficiency
Authors: Eom Kisang , Lee Hyoung Ro , Hyun Jung Ho , An Hyunhoe , Lee Yong‐Seok , Ho Won‐Kyung , Lee Suk‐Ho 
Published: December 2021

AbstractThe associative network of hippocampal CA3 is thought to contribute to rapid formation of contextual memory from one‐trial learning, but the network mechanisms underlying decorrelation of neuronal ensembles in CA3 is largely unknown. Kv1.2 expressions in rodent CA3 pyramidal cells (CA3‐PCs) are polarized to distal apical dendrites, and its downregulation specifically enhances dendritic responses to perforant pathway (PP) synaptic inputs. We found that haploinsufficiency of Kv1.2 (Kcna2+/−) in CA3‐PCs, but not Kv1.1 (Kcna1+/−), lowers the threshold for long‐term potentiation (LTP) at PP‐CA3 synapses, and that the Kcna2+/− mice are normal in discrimination of distinct contexts but impaired in discrimination of similar but slightly distinct contexts. We further examined the neuronal ensembles in CA3 and dentate gyrus (DG), which represent the two similar contexts using in situ hybridization of immediate early genes, Homer1a and Arc. The size and overlap of CA3 ensembles activated by the first visit to the similar contexts were not different between wild type and Kcna2+/− mice, but these ensemble parameters diverged over training days between genotypes, suggesting that abnormal plastic changes at PP‐CA3 synapses of Kcna2+/− mice is responsible for the impaired pattern separation. Unlike CA3, DG ensembles were not different between two genotype mice. The DG ensembles were already separated on the first day, and their overlap did not further evolve. Eventually, the Kcna2+/− mice exhibited larger CA3 ensemble size and overlap upon retrieval of two contexts, compared to wild type or Kcna1+/− mice. These results suggest that sparse LTP at PP‐CA3 synapse probably supervised by mossy fiber inputs is essential for gradual decorrelation of CA3 ensembles.
Cytoreductive robot‐assisted prostatectomy for systemic prostate rhabdomyosarcoma presenting as urinary retention
Authors: Kirisawa Takahiro , Fukunaga Arinobu , Takamori Hajime , Maejima Aiko , Shinoda Yasuo , Komiyama Motokiyo , Fujimoto Hiroyuki , Yonemori Kan , Yoshida Akihiko , Matsui Yoshiyuki 
Published: December 2021

IntroductionIn adults, rhabdomyosarcoma of the prostate is extremely rare and has an unfavorable prognosis. These patients frequently experience urinary obstruction, and cysto‐prostatectomy is a mainstay treatment for localized disease. In contrast, treatment strategies for the primary site for metastatic disease remain controversial. To our knowledge, robot‐assisted surgery for the primary tumor has not been reported.Case presentationA 26‐year‐old man complained of dysuria. Magnetic resonance imaging showed enlarged prostate and computed tomography revealed a pulmonary metastasis. Transurethral resection of the prostate led to the diagnosis of rhabdomyosarcoma. After chemotherapy, robot‐assisted prostatectomy was performed to relieve obstructive urinary symptoms. Although disease progression in the metastatic site was observed after the surgery, urinary obstruction did not occur and quality of life was well maintained.ConclusionRobot‐assisted prostatectomy may be beneficial both for local disease control and palliation of voiding impairment among selected patients with systemic rhabdomyosarcoma of the prostate.
Significance of serum miR‐29a in the occurrence and progression of diabetic nephropathy: A cross‐sectional study
Authors: Liu Qian , Wang Menglin , Xu Tongdao , Liang Wei , Yang Fumeng 
Published: December 2021

AbstractBackgroundDiabetic nephropathy (DN), a common microvascular complication of type 2 diabetes mellitus (T2DM), is an important factor causing chronic kidney disease. However, the relationship between miR‐29a and DN remains unknown. Therefore, a cross‐sectional study was conducted to identify a potential molecular biomarker for DN prevention and management by detecting the serum miR‐29a levels.MethodsThe serum miR‐29a levels were measured in 360 subjects (180 T2DM patients and 180 healthy controls) using quantitative reverse transcription PCR (qRT‐PCR), and other conventional indicators were measured and analysed. A binary logistic regression was used to evaluate the DN risk factors; a receiver operating characteristic (ROC) curve was applied to analyse the diagnostic efficacy of miR‐29a for DN, and a Spearman's rank correlation analysis was used to evaluate the correlation between serum miR‐29a and cystatin C.ResultsThe serum miR‐29 levels in the T2DM patients were higher than those in the healthy subjects and significantly increased with the progression of DN (p < 0.05). Serum miR‐29a and cystatin C are independent predictors of the occurrence of DN. Compared with a single indicator, the combination of serum miR‐29a and cystatin C has better DN diagnostic performance. In addition, the serum miR‐29a levels were positively correlated with cystatin C in the patients with DN (r = 0.521, p < 0.001).ConclusionThe expression of serum miR‐29a was significantly associated with the occurrence and progression of DN and is expected to become a potential biomarker for the diagnosis of DN.