bluuebunny/crossref_metadata_2025_split huggingface dataset:
Toward Proteomics in Uroscopy: Urinary Protein Profiles after Radiocontrast Medium Administration
Authors: HAMPEL DIERK J. , SANSOME CHRISTINE , SHA MA , BRODSKY SERGEY , LAWSON WILLIAM E. , GOLIGORSKY MICHAEL S. 
Published: April 2021

Abstract.
Previous attempts to use urinary protein profiles for diagnostic purposes have been rather disappointing with respect to their clinical validity, in part because of the insufficient reproducibility, sensitivity, and rapidity of available techniques. Therefore, a newly developed, high-throughput technique, namely surface-enhanced laser desorption/ionization (SELDI) ProteinChip array-time of flight mass spectrometry, was studied, to assess its applicability for protein profiling of urine and to exemplify its use for a group of patients receiving radiocontrast medium. Assessment of the accuracy, sensitivity, and reproducibility of SELDI in test urinary protein profiling was performed. Renal function was studied in 20 male Sprague-Dawley rats before and after intravenous administration of either 1.25 g/kg ioxilan (n = 10) or hypertonic saline solution (n = 10) as a control. Urine samples from 25 patients undergoing cardiac catheterization were obtained before, immediately after, and 6 to 12 h after the procedure. Administration of ioxilan to rats resulted in changes in the abundance of proteins of 9.9, 18.7, 21.0, and 66.3 kD. For patients, even in uncomplicated cases of radiocontrast medium infusion during cardiac catheterization, perturbations in the protein composition occurred but returned to baseline values after 6 to 12 h. Proteins with molecular masses of 9.75, 11.75, 23.5, and 66.4 kD changed in abundance. For patients with impaired renal function, these changes were not reversible within 6 to 12 h. As a proof of principle, one of the peaks, i.e., that at 11.75 kD, was identified as β2-microglobulin. SELDI is a promising tool for the detection, identification, and characterization of trace amounts of proteins in urine. Even for patients without renal complications, proteins with a broad range of molecular masses either appear in or disappear from the urine. Some of these might represent markers of impending nephropathy.
Specific Antagonism of PDGF Prevents Renal Scarring in Experimental Glomerulonephritis
Authors: OSTENDORF TAMMO , KUNTER UTA , GRÖNE HERMANN JOSEPH , BAHLMANN FERDINAND , KAWACHI HIROSHI , SHIMIZU FUJIO , KOCH KARL MARTIN , JANJIC NEBOJSA , FLOEGE JÜRGEN 
Published: April 2021

Abstract.
Glomerular mesangial cell proliferation and/or mesangial matrix accumulation characterizes many progressive renal diseases. Rats with progressive mesangioproliferative glomerulonephritis were treated from day 3 to day 7 after disease induction with a high-affinity oligonucleotide aptamerantagonist against platelet-derived growth factor-B chain (PDGF-B). In comparison with nephritic rats that received vehicle or a scrambled aptamer, treatment with the PDGF-B aptamer led to a significant reduction of mesangioproliferative changes, glomerular hypertrophy, podocyte damage, and glomerular macrophage influx on day 8. Both nephritic control groups subsequently developed progressive proteinuria and decreased renal function. On day 100, glomerulosclerosis, tubulointerstitial damage, glomerular and interstitial accumulation of types III and IV collagen, and overexpression of transforming growth factor-β were widespread. All of these chronic changes were prevented in rats that received the PDGF-B aptamer, and their functional and morphologic parameters on day 100 were largely indistinguishable from non-nephritic rats. These data provide the first evidence for a causal role of PDGF in the pathogenesis of renal scarring and point to a new, highly effective therapeutic approach to progressive, in particular mesangioproliferative, renal disease.
A Novel Approach to Investigation of the Pathogenesis of Active Minimal-Change Nephrotic Syndrome Using Subtracted cDNA Library Screening
Authors: Sahali Djillali , Pawlak André , Valanciuté Asta , Grimbert Philippe , Lang Philippe , Remy Philippe , Bensman Albert , Guelle[Combining Diaeresis]n Georges 
Published: April 2021

ABSTRACT.
Clinical and experimental observations suggest that minimal-change nephrotic syndrome (MCNS) results from T cell dysfunction, via unknown mechanisms. For the identification of genes that are potentially involved in MCNS, a subtractive cDNA library was constructed from cDNA from T cell-enriched peripheral blood mononuclear cells obtained from the same patient during relapse versus remission (“relapse minus remission”). This library was screened by differential hybridization with forward (“relapse minus remission”) and reverse (“remission minus relapse”) subtractive cDNAs probes, as well as unsubtracted probes from relapse and remission, and irrelevant nephrotic syndrome (membranous nephropathy). A total of 84 transcripts were isolated, of which 12 matched proteins of unknown function and 30 were unknown clones. Among the 42 known transcripts, at least 18 are closely involved in the T cell receptor-mediated complex signaling cascade, including genes encoding components of the T cell receptor and proteins associated with the cytoskeletal scaffold, as well as transcription factors. In particular, it was demonstrated that the expression levels of Fyb/Slap, L-plastin, and grancalcin were increased during relapse, suggesting that the integration of proximal signaling after T cell engagement involves the preferential recruitment of these cytoskeleton-associated proteins in MCNS. Because very low levels of interleukin-12 receptor β2 mRNA were detected in relapse samples, the interleukin-12 signaling pathway might be defective, suggesting that, in MCNS, T cell activation evolves toward a T helper 2 phenotype. Therefore, the combination of subtractive cloning and differential screening constitutes an efficient approach to the identification of genes that are likely to be involved in the pathophysiologic processes of MCNS.
PEDAGOGIA - PENSAR A PEDAGOGIA PARA RESPONDER AOS CONTEXTOS ESCOLARES E NÃO ESCOLARES DA ATUALIDADE
Authors: Medeiros Emerson Augusto de , Araújo Osmar Hélio Alves , Ferreira Liliana Soares 
Published: April 2021

Na literatura da área de Educação, internacional e nacional, temos identificado, na história, o debate acerca da Pedagogia como ciência da educação. No Brasil, particularmente, as discussões produzidas nos últimos anos, em periódicos científicos, teses, dissertações, livros, eventos acadêmicos, entre outros, se endereçam a partir da defesa de que é a Pedagogia a ciência que estuda, problematiza, compreende e reflete sobre a natureza dos fenômenos educativos (PIMENTA, 2012). No entanto, conforme consta no estudo de doutoramento da Professora Maria Amélia Santoro Franco, publicizado no livro “Pedagogia como ciência da Educação”, pouco a pouco, a Pedagogia tem perdido seu lugar, na condição de ciência, e tem se secundarizado, tornando-se apenas uma área da Educação (FRANCO, 2008). Isso ocorre, principalmente, em razão de que, ao longo do tempo, a Pedagogia precisou adequar-se à lógica subjacente às ciências positivistas (FRANCO, 2008; FRANCO, 2011). Em virtude desse cenário, muitos profissionais da educação a desconhecem, mesmo aqueles que atuam em cursos de Pedagogia ou desenvolveram suas formações iniciais nessas graduações (LIBÂNEO, 2007; FRANCO, 2008).
Metastases of papillary radioiodine-refractory thyroid cancer in the parapharyngeal lymph node: clinical case
Authors: Ognerubov N. A. , Antipova T. S. 
Published: April 2021

Introduction. Papillary thyroid cancer (PTC) usually metastasizes into the central and lateral lymph nodes (LNs) of the neck. Metastases into the retropharyngeal and parapharyngeal LNs are rare. Their presence attests to aggressive PTC.The study objective is to describe a rare case of metastases of papillary radioiodine-refractory PTC into the parapharyngeal LN.Clinical case. In 2015, female patient K., 40 years old, underwent thyroidectomy due to PTC. Histological examination verified papillary PTC with growth through the capsule and ingrowth into the surrounding tissues and muscles. In a separately admitted LN, metastases of the same cancer were observed. One year later, regional metastases in the lateral neck LNs were detected. Radioiodine therapy (activity 131I 4.5 GBq) was performed followed by fascial circular section of the neck tissues on the right per thyroid type. Morphological examination verified presence of papillary PTC metastases in 4 LNs. In January of 2018, positron emission tomography showed metastases in the paratracheal LNs. Central neck lymph node dissection was performed. Per histological conclusion, fat tissue and LN contained multiple metastases of papillary PTC. In October of the same year, repeat radioiodine therapy (activity 131I 3.0 GBq) was performed. Thyroglobulin levels increased. In June of 2020, repeat positron emission tomography showed a single metastasis in the parapharyngeal LN. Due to small size of the metastasis and absence of signs of progression, dynamic follow-up and hormone therapy were suggested to the patient.Conclusion. Metastatic involvement of parapharyngeal LNs is rare, especially in radioiodine-refractory PTC. They can be detected both during primary diagnosis and after the treatment during dynamic follow-up, as well as a single manifestation of PTC, which should be taken into account during differential diagnosis.
New opportunities for preoperative diagnosis of anaplastic thyroid cancer
Authors: Lukyanov S. A. , Sergiyko S. V. , Titov S. E. , Veryaskina Yu. A. , Mudunov A. M. , Dobrokhotova V. Z. , Kozorezova E. S. , Vorobyov S. L. , Vazhenin A. V. , Romanchishen A. F. , Vabalaite K. V. , Vilkova A. S. , Timofeeva N. I. , Ilinа T. E. 
Published: April 2021

Background. Anaplastic thyroid cancer (ATC) is one of the most aggressive human tumors. Since median survival of ATC patients is only 4 months, its early diagnosis is very important. Although ATC has specific clinical manifestations, the analysis of expression of different microRNAs can facilitate preoperative diagnostics and help to detect its potential precursors among differentiated cancers and other thyroid malignancies.The study objective is to identify microRNAs specific for ATC that are different from microRNAs in other thyroid cancers.Materials and methods. We analyzed the expression of 14 microRNAs in histological specimens of 67 patients with ATC. The control groups included 25 patients with benign nodules, 36 patients with follicular adenomas, 32 patients with follicular cancer, and 152 patients with papillary thyroid cancer. For 7 out of 67 ATC patients, we compared mi-croRNA levels in histological and cytological specimens.Results. Patients with ATC demonstrated a statistically significant decrease in the expression of miR-145, miR-125b and increase in the expression of miR-155 and miR-21 compared to all control groups. We found two reliable diagnostic markers of ATC: relative miR-21 expression (at a cutoff of 14.9, sensitivity was 0.955 and specificity was 0.837) and the miR-21/miR-145 ratio (at a cutoff of 122, sensitivity was 0.955 and specificity was 0.955). The level of miR-21 expression and miR-21/miR-145 ratio in cytological specimens were accurate in all 7 cases (100 %).Conclusion. the level of expression of specific microRNAs can be used as a reliable biomarker for ATC. The consistency between the results obtained in cytological and histological specimens enables the use of stained cytological samples for this analysis.
Capabilities of fluorescent spectroscopy in diagnosis of tumors of the ent-organs
Authors: Daikhes N. A. , Vinogradov V. V. , Kim I. A. , Reshulsky S. S. , Prikuls V. F. , Karneeva O. V. , Khabazova A. M. , Prykule D. V. 
Published: April 2021

Introduction. Diagnosis verification in patients with malignant tumors developed in the context of chronic inflammation is technically complex with a high percentage of false negative results of histological examination. None of the methods provides sufficiently accurate results necessary for ruling out or confirming a diagnosis.The study objective is to test a new patented method of pre- and intraoperative differential diagnosis of oncological pathology of the ent-organs using the "InSpektr-M" hardware and software system.Materials and methods. The study included 30 patients with suspected malignant tumors of the larynx. The study excluded patients with previously verified diagnosis of malignant tumor of the larynx. A diagnostic probe emitting laser light with wavelength 350 nm and radiation power density 25 mW/cm2 was introduced through an additional channel of a fiberscope for a period of time sufficient for excitation of intrinsic tissue fluorescence (from 10-6 to 10-9 ms). Fluorescence intensity in the range of wavelengths between 600 and 650 nm was measured. This intensity was conditionally labelled "epithelial proliferative activity index". Spectrometric analysis of the intact points allowed to evaluate normal values for an individual patient; in the areas with the maximal increase in the index, targeted biopsy was performed.Results. Values obtained during examination of various types of tumors (epithelial and non-epithelial) are different while tumor location has a negligible effect. In patients with malignant tumors containing non-squamous epithelium, increased protoporphyrin IX and decreased FAD fluorescence were observed in the direction from intact tissues to the center of the suspected tumor; in patients with malignant tumors containing squamous epithelium: increased protoporphyrin IX fluorescence with maximum at 635 nm and dynamic increase in FAD fluorescence in the direction from intact tissues to the tumor center.Conclusion. Fluorescent spectroscopy can be used for pre- and intraoperative differential diagnosis of squamous and non-squamous cancers of the ENT-organs. Further studies for establishment of reference values of fluorescence intensity in intact and affected tissues are necessary.
Yellow Pumpkin (Cucurbita maxima D.) Extract As Anti-Hypercolesterolemia
Authors: Sunnah Istianatus , Kustiyaningsih Erna , Oktianti Dian 
Published: April 2021

Hyperlipidemia is a risk factor for various diseases, which is still a big problem in Indonesia. Yellow pumpkin (Cucurbita maxima D.) is a plant containing flavonoids and terpenoids, which can be used as an anti-hypercholesterolemic agent. This study aims to analyze the activity and dosage of yellow pumpkin extract, which can be used as an anti-hypercholesterolemic agent to reduce total cholesterol levels comparable to simvastatin by 1.8mg/kg BW/day. This research is pure experimental research with a pre and post-test group design approach. The number of samples was 25 male Wistar rats induced by high-fat feed, quail egg yolk: pork oil (2:1) by 5 ml/200 gramBW/day for 7 days. The extract dosage range was 200, 400, 600 mg/kg BW/ day for 7 days. Data were analyzed using One Way ANOVA. The results showed that the administration of yellow pumpkin extract could reduce rats' total blood cholesterol levels with a dose of 600 mg/kg BW/day, comparable to simvastatin 1.8 mg/kg BW/day. The secondary metabolites of the extract were flavonoids and terpenoids. Extract of yellow pumpkin (Cucurbita maxima D.) could reduce total blood cholesterol levels of rats. The dose of 600 mg/kg BW/day could reduce blood cholesterol levels in rats comparable to simvastatin 1.8 mg/kg BW / day.
Pattern of skin diseases among patients attending Hawassa university comprehensive specialized hospital, Hawassa, Ethiopia
Authors: Assefa Girum T. , Koster Adane C. , Nurhussein Nura K. 
Published: April 2021

<p><strong>Background</strong>: Spectrum of skin diseases varies from region to region due to several factors such as genetics, socioeconomic and environmental. This study aimed to determine the pattern of various skin disorders appearing in the dermatology department of a tertiary care hospital.</p><p><strong>Methods</strong>: All patients attending the dermatology clinic of the Hawassa university comprehensive specialized hospital, from January 2017 to December 2018 were included in this retrospective analysis. The medical records of the patients were obtained from registry books. </p><p><strong>Results: </strong>A total of 7727 patients attended the dermatology clinic of HUCSH. Out of these samples, 18.1% of patients repeatedly came to the hospital for a follow up visit related to their diseases, while 81.9% were enrolled as new cases.</p><p>There were more males (51.9%) than females. More than three quarter of the cases were aged above 16 years while less than a quarter were children below 16 years. Eczema was diagnose in 2734 (35.4%), being the most common cause for attendance, followed by infectious disease (23.3%) and disease of the skin appendages (12.1%).</p><p class="abstract"><strong>Conclusions</strong>: Eczema was the most common skin disease seen in our study, followed by infectious disease and disease of the skin appendages. Concerted effort need to be made to control these conditions and training of the primary health care providers and education of General Practitioners in Dermatology must emphasize these common conditions, with the aim of improving primary health care and alleviating the burden of hospital care.</p>
Posthumanism: A Desire for a New Humanity
Authors: Sidebottom Kay , Lee Carol , Fairchild Nikki 
Published: April 2021

Call for Submissions for the Summer, 2022 issue of Cultural and Pedagogical Enquiry for the Special Issue: Posthumanism: A Desire for a New Humanity with Guest Editors Kay Sidebottom, Carol Lee, and Nikki Fairchild.
El movimiento estudiantil en la Universidad Pedagógica y Tecnológica de Colombia 1971
Authors: Bernal Villate Sandra Liliana , Patiño Diego Eduardo Naranjo 
Published: April 2021

El Objetivo: de este trabajo se centra, en el análisis del movimiento estudiantil de la Universidad Pedagógica y Tecnológica de Colombia, en el año 1971.
Originalidad/aporte: se centraliza, en la movilización y planteamientos de reforma estudiantil por conseguir el cogobierno universitario, que realizaron los grupos políticos de izquierda, en esta institución.
Método: se inscribió en la historia política
Estrategia/recolección de información: se enfocaron en la recuperación de la memoria de sus protagonistas, contrastada con la revisión de las Actas de los consejos académico y superior y las publicaciones de la prensa nacional sobre el tema de las movilizaciones estudiantiles.
Conclusiones: se puede concluir que, en esta universidad, las divisiones internas de los colectivos estudiantiles, junto con las políticas represivas de expulsión y la no vinculación del sector social y sindical a movimiento, incidieron en el hecho de no lograr una negociación local que permitiera alcanzar el sueño del cogobierno universitario, que sólo se lograría en Colombia, hasta el año 1991, con el cambio de la Constitución política del país.
Globalização e teoria social contemporânea
Authors: Arnaut Danilo 
Published: April 2021

O propósito deste artigo é discutir alguns dos desafios metodológicos enfrentados no desenvolvimento de minhas pesquisas sobre a problemática da globalização nas ciências sociais. O que segue, contudo, não corresponde a um conjunto de teorias, teses ou diagnósticos a respeito da globalidade emergente. De certo modo, aliás, a globalização, em si, nem mesmo constitui o objeto principal desta reflexão: a intenção é investigar as trilhas de pensamento e sensibilidade percorridas pelos estudiosos da globalização – em especial, Octavio Ianni e Ulrich Beck, que foram os escolhidos na composição do recorte investigativo. Esta reflexão será dividida em quatro partes. Primeiro, trato da própria construção do objeto da pesquisa. Em seguida, cabe abordar também a construção da própria pesquisa. Com base nisso, procuro compartilhar algumas das dificuldades procedimentais no trato dos escritos trabalhados, buscando apresentar alguns caminhos metodológicos para o seu enfrentamento e também para a compreensão do seu status teórico-epistemológico. Por fim, reflito sobre a prática interpretativa de explicações sociológicas como as abordadas aqui.
Apresentação
Authors: Paniz Flávia , Oliveira Gláucia Silva Destro de 
Published: April 2021

A proposta de organizar este dossiê surgiu no segundo semestre de 2016, como forma de criar um registro das diferentes perspectivas sobre os acontecimentos ocorridos ao longo do ano, e que pudesse ser utilizado como apoio nas pesquisas e reflexões futuras sobre este período, que ficou historicamente marcado pelo processo de votação do impeachment da ex presidenta eleita Dilma Rousseff, seguido de seu afastamento; pelos protestos contra a realização da copa do mundo; e a prisão de Rafael Braga, um catador de material reciclável que portava uma garrafa de produto de limpeza no contexto dos protestos no Rio de Janeiro. Estes últimos fatos, juntamente com o desaparecimento do pedreiro Amarildo de Souza em 2013 e o assassinato de Claudia Silva Ferreira em 2014, escandalizaram a situação de encarceramento em massa e genocídio da população negra e periférica e o processo de precarização no ensino público no país.
O corte e a corte
Authors: Silveira Ana Cláudia Lopes , Pinheiro Hyury , Pagan Maria Elisa Perez , Borges Mariana Toledo , Costa Nathalia Rodrigues da , Queiroz Pedro Henrique Santos 
Published: April 2021

O objetivo deste texto é contribuir para o debate sobre os cortes no orçamento da Universidade Estadual de Campinas propostos pela reitoria no ano de 2016. Pretendese refletir sobre tal contingenciamento à luz da estrutura orçamentária em geral. Primeiro, apresenta-se o quadro atual do financiamento da Universidade Pública, e, particularmente, da Unicamp. Em seguida, discute-se esse quadro tendo em vista a estrutura tributária mais geral e das soluções de resolução apresentadas para o déficit orçamentário. Buscamos aqui pensar esses cortes no orçamento relacionando-os à atual conjuntura econômica, sem perder de vista a estrutura do financiamento do ensino universitário público. Com essa reflexão, pretendemos trazer mais subsídios para o debate sem abrir mão das demandas em favor de políticas de financiamento e de um projeto de ensino democrático e a serviço do público.
Analisa Kekuatan Struktur Barge Pada Proses Load Out Offshore Module (Top Side) dengan SPMT
Authors: Palippui Habibi , Ramadhan Sahwan 
Published: April 2021

Salah satu proses yang kritikal ketika fabrikasi offshore yaitu proses transfer offshore module (Top Side) dari atas jetty ke atas transportation barge atau sering disebut Proses Load Out. Salah satu analisa yang dibutuhkan untuk memastikan proses load out berjalan dengan lancar dan aman yaitu analisa kekuatan struktur barge pada saat proses load out tersebut terjadi yaitu dimana dilakukan pengecekan terhadap kekuatan struktur barge apakah mampu menahan beban top side ketika terjadi transfer beban dari atas jetty ke atas transportation barge atau dari tepi barge sampai ke final lokasi. Untuk mendapatkan hasil yang akurat maka digunakan bantuan program komputer atau sering disebut dengan metode elemen hingga (Finite Element Analysis). Adapun software yang digunakan pada analisa ini yaitu StaadPro V8i dengan simulasi percobaan ada tiga module. Dari hasil analisa diperoleh kesimpulan bahwa simulasi percobaan module tiga memiliki nilai maksimum von misses stress adalah 74,119 N/mm2, normal stress adalah 57,765 N/mm2, dan shear stress adalah 0,02279 N/mm2, dimana semua nilai tegangan tersebut dibawah tegangan izin atau dibawah batas maksimal yang disyaratkan AISC sehingga struktur barge tidak perlu diberikan tambahan penguatan baik internal maupun eksternal karena masih aman dan mampu untuk menahan beban top side ketika load. Analisis dengan SPMT berpotensi menjadi patokan dalam dalam menganalisis kekuatan struktrur barge dalam proses load out offshore module (top side).