bluuebunny/crossref_metadata_2025_split huggingface dataset:
Suppression of Quercetin-Induced Autophagy Enhances Cytotoxicity through Elevating Apoptotic Cell Death in Human Bladder Cancer Cells
Authors: Tsai Te-Fu , Hwang Thomas I-Sheng , Lin Ji-Fan , Chen Hung-En , Yang Shan-Che , Lin Yi-Chia , Chou Kuang-Yu 
Published: December 2021

Objective:
Quercetin, a natural dietary compound, has been demonstrated with antitumor activities against several types of cancers by disrupting cell cycle and inducing apoptotic cell death. However, human bladder cancer cells such as 5637 and T24 cells expressing mutant p53 are resistant to a 24 hrs quercetin treatment. In this study, the anticancer effect of quercetin was evaluated in these bladder cancer cells.

Materials and Methods:
The bladder cancer cells treated with quercetin were subjected to evaluated cell apoptosis by caspase activity, TUNEL assay and cell viability assay. The cell autophagy was assessed by detecting procession of LC3-II autophagic marker protein.

Results:
After 48 and 72 hrs of incubation, quercetin was found to be significantly effective in inhibiting proliferation of 5637 and T24 cells in a dose-dependent manner. Quercetin treatment increased the caspase 3/7 activities, percentage of subG0/G1 cells, and DNA fragmentation, indicating an induced apoptotic cell death. Pretreatment of a pan-caspase inhibitor, Z-VAD-FMK, attenuated the quercetin-decreased cell viability, suggesting that the cytotoxicity caused by quercetin mainly via apoptotic cell death. We also found that quercetin induced autophagy, as evidenced by the increased processing of LC3-II, a specific marker of autophagy. The disruption of autophagic flux by using bafilomycin A1, an autophagy inhibitor, caused significant accumulation of cellular p62 and LC3-II. In addition, the pretreatment of autophagy inhibitors, Baf A1 and chloroquine, strongly augmented apoptosis in 5637 and T24 cells, indicating the suppression of quercetin-induced autophagy enhanced apoptosis. Furthermore, the decreased cell viability and increased LC3-II processing were attenuated in quercetin-treated cells which pretreated with a reactive oxygen species (ROS) scavenger, N-acetyl cystine (NAC) suggested that quercetin-induced cytotoxicity and autophagy were initiated by the generation of ROS.

Conclusion:
This study proposes that combined treatment of autophagy inhibitor which sensitizes cells to quercetin treatment may be a better therapeutic approach to reduce bladder cancer cells proliferation.
Outcome Analysis of Pediatric Pyeloplasty in Varied Presentation in Developing Countries
Authors: Khemchandani Sajni I. 
Published: December 2021

Objectives:
The objective was to study the various presentations of pelviureteric junction (PUJ) obstruction in developing countries and its outcomes.

Materials and Methods:
We retrospectively reviewed the medical records of 239 children (260 renal units) who underwent surgery for PUJ obstruction between January 1994 and December 2016. Data on the patients presenting with symptoms and preoperative imaging findings of ultrasonography, intravenous urography, or diuretic renography were analyzed.

Results:
In all cases, we preferred dismembered Anderson-Hynes pyeloplasty with or without JJ stenting. Complications of pyeloplasty included prolonged urine leakage in 13 patients, of whom 4 responded to conservative management, 5 required percutaneous nephrostomy (PCN), and 4 were treated with endoscopic JJ stenting. Six patients had persistent obstruction: two responded to endopyelotomy, and in four, redo pyeloplasty was successful. Five patients with renal function of <10% were initially treated with PCN and ultimately required nephrectomy.

Conclusions:
In patients born with PUJ obstruction and impaired renal function, pyeloplasty fails to significantly improve function, possibly because of cortical loss. However, in patients with antenatal PUJ obstruction managed expectantly, there is a small but significant risk of modest permanent loss of renal function.
Genotyping and Molecular Characterization of Extended-Spectrum Beta-Lactamases-Producing Uropathogenic Escherichia Coli in and Around Coimbatore District, Tamil Nadu, India
Authors: Muraleetharan Mylsamy , Viswanathan Thirumoorthy 
Published: December 2021

Background:

Escherichia coli, an extraintestinal flora, develops virulence traits through a persistent encounter with host and constant exposure to antibiotics, which makes it line up as the most common urinary pathogen. Thus, this study aimed to screen the presence of virulence traits among multidrug-resistant urinary pathogenic E. coli among the urine samples collected from inpatients and outpatients of the multispecialty hospitals.

Materials and Methods:
Standard microbiological laboratory protocols were followed, and about 210 samples were processed and screened.

Results:
Among those samples, 114 were reported positive for the presence of uropathogenic E. coli(UPEC). Gender-wise distribution was found to be more among female patients (56%) than male patients (51%). During screening for hemolytic activity, 37% of test isolates were α hemolytic, 48% were β hemolytic, and 15% were γ hemolytic. Screening virulence among test isolates, sfa gene (49%), afa (42%), hly (68%), CNF (43%), and aer, accounts for 65%. Further, the multidrug resistance of the isolates was done by using ten antibiotics. All the isolates exhibited the multiple antibiotic resistance (MAR), and the highest percentage of resistance was found against ceftazidime (100%), and the least percentage of resistance was observed against imipenem (2%) followed by amikacin (8%). MAR index values of all the isolates ranged from 0.4 to 1.

Conclusion:
The presence of various virulence genes and the high degree of resistance among the isolates against the antibiotics used in this study confirm the prevalence of extended-spectrum β-lactamase producers among the UPEC.
The Associations of Genital-Normalizing Surgery and Assigned Gender in Predicting Gender Outcomes
Authors: Veale Jaimie F. 
Published: December 2021

Purpose:
The purpose was to systematically analyze the associations of childhood gender assignment and genital normalizing surgeries with adult gender in case reports of adults with differences of sex development (DSD). We did this using a pooled nested analysis of DSD adult cases reported in the academic literature.

Materials and Methods:
A search of academic databases uncovered 282 adult DSD cases reported in 58 academic articles that met the criteria for inclusion in the analysis. These were cases with 46, XX congenital adrenal hyperplasia, partial androgen insensitivity, mixed or partial gonadal dysgenesis, and micropenis where data about the assigned gender, childhood genital-normalizing surgery, and adult gender could be extracted. We conducted a generalized logistic mixed-model regression analysis, with multiple predictors of adult assigned gender incongruence.

Results:
Controlling for assigned gender, age, year of article publication, and DSD type, childhood genital surgery was not significantly associated with adult assigned gender incongruence. Cases assigned female had more than five times greater likelihood of assigned gender incongruence (95% confidence interval = 1.96–14.92).

Conclusion:
This study did not find evidence that childhood genital surgery is related to assigned gender incongruence; it also found that assigning a child as a female increased this possibility. These findings may inform decisions taken by clinicians and family members about the early management of DSD cases. The study was limited by publication bias in the types of cases that get published. Ethical considerations should always take precedence in decision-making regarding these surgeries.
Is Repeated Direct Vision Internal Urethrotomy Feasible in the Management of Recurrent Anterior Urethral Strictures?
Authors: Chung Cho-Hsing , Wu Wen Ling , Liu Yin-Ting , Tzou Kai-Yi , Hu Su-Wei , Chiang Yi-Te , Ho Chen-Hsun , Liu Chia-Hung , Wu Chia-Chang , Chen Kuan-Chou , Kao Wei-Tang 
Published: December 2021

Purpose:
Direct vision internal urethrotomy (DVIU) is the most common surgical intervention for anterior urethral strictures. We evaluated the long-term outcome and success rate of optic urethrotomy in patients treated in our hospital who had recurrent anterior urethral strictures, according to different predisposing causes and stricture sites. The aim is to find the success rate of optic urethrotomy in patients treated who had recurrent anterior urethral strictures.

Materials and Methods:
This study is a retrospective chart review. From January 2000 to August 2017, 232 patients received internal optic urethrotomy for anterior urethral strictures in our hospital. A retrospective chart review of all patients involved in the study was performed. Median follow-up period after receiving the first urethrotomy procedure was 91 months (range: 8–204 months). The Kaplan–Meier method was used to analyze the success rate of the first, second, third, fourth, and fifth urethrotomy procedures.

Results:
Of the 232 patients who underwent at least two procedures of urethrotomy, 150 (64.65%) were included in the study. The success rate of the first urethrotomy procedure was 0%, with a mean time to recurrence of 20 months. For the second urethrotomy, the success rate was 53%, with a mean time to recurrence of 22.5 months. For the third urethrotomy, the success rate was 40%, with a mean time to recurrence of 16.1 months. For the fourth and fifth urethrotomy procedures, the success rate was 39% and 18%, with a mean time to recurrence of 17.9 and 10.2 months, respectively. Patients who had anterior urethral stricture related to previous surgery (surgery-related) had a better outcome (success rate, 87.3%) than patients with strictures stemming from other causes (P = 0.003). No association was found between preoperative infection, the age of the patient, and the site of the strictures and the success rate of the procedure.

Conclusion:
The patient population was older and had a longer-term follow-up, compared with previous studies, but even with repeated DVIU, the surgery-related stricture group had a higher success rate of urethrotomy than the groups with different etiology. In patients with pelvic fracture or infection, repeated urethrotomy may not be recommended as first-line treatment for recurrent anterior urethral stricture.
Initial Prostate Biopsy of Grade Group One
Authors: Hung Fan-Ching , Tseng Chi-Shin , Chen Chung-Hsin , Chang Hong-Chiang , Huang Chao-Yuan , Lu Yu-Chuan 
Published: December 2021

Purpose:
The aim of our study was to analyze the outcomes and predictive factors in patients with an initial biopsy grade group 1 (GG1) prostate cancer (PCa) at risk of GG upgrading.

Materials and Methods:
We performed a retrospective review of patients who had GG1 PCa at biopsy and were managed with robotic-assisted laparoscopic radical prostatectomy (RaLRP) from January 2012 to December 2018 and collected their clinical characteristics and pathological data. The primary outcomes were GG upgrading at RaLRP and biochemical recurrence-free survival (BCRFS) in these patients. The secondary outcome was to analyze the risk factors of pathological upgrades.

Results:
Among the 159 patients with initial prostate biopsy of GG1, 122 patients (76.7%) had GG upgrading based on the final pathology at RaLRP. Multivariable analysis showed that transrectal ultrasound (TRUS)-measured prostate volume <30 mL (odds ratio [OR] 4.727, P = 0.011), prostate-specific antigen density [PSAD] ≥0.2 ng/mL2 (OR 3.201, P = 0.019), magnetic resonance imaging (MRI)-measured prostate volume <30 mL (OR 3.892, P = 0.007), and PSAD ≥ 0.2 ng/mL2 (OR 2.65, P = 0.019) were independent predictive factors of GG upgrading. During 5 years of follow-up, patients who upgraded to GG3-5 had a significantly shorter time to biochemical recurrence than those who remained as GG1 (P = 0.001) or upgraded to GG2 (P = 0.008).

Conclusion:
The final pathology grading was underestimated in 76.7% of initial biopsy GG1 and may influence the BCRFS after RaLRP. Prostate volume <30 mL and PSAD ≥0.2 ng/mL2, measured by either MRI or TRUS, were significant predictive factors of biopsy GG1 upgrading.
STRATEGI PROMOSI DALAM MENINGKATKAN PENJUALAN PRODUK MARIA SNACK
Authors: Maria Sendi  , Vuspitasari Benedhikta Kikky 
Published: December 2021

Di era modern ini, banyak masyarakat yang mencari Snack unik dan tentunya memiliki cita rasa yang berbeda dengan yang lainnya. Tujuan Penelitian Skripsi Dengan Judul Strategi Promosi Dalam Meningkatkan Penjualan Produk Maria Snack adalah untuk mengetahui strategi promosi yang tepat digunakan oleh Maria Snack untuk meningkatkan penjualan produk dari Maria Snack. Penelitan ini menggunakan metode penelitian kualitatif, dengan teknik pengumpulan data yang dilakukan dengan wawancara, observasi, dan dokumentasi. Dalam mendeskripsikan hasil penelitian menggunakan analisis SWOT dengan menganalisis faktor internal dan eksternal dari strategi promosi yang dilakukan dengan memberikan gambaran umum mengenai kekuatan, kelemahan, peluang serta ancaman dari strategi promosi yang dilakukan oleh Maria Snack. Berdasarkan hasil penelitian dan pembahasan di atas, jadi dapat disimpulkan antara lain: 1. Analisis faktor internal dan eksternal strategi promosi yang diterapkan Oleh Maria Snack menggunakan matriks SWOT menjadi 9(sembilan) alternatif strategi dilihat dari strategi SO, strategi WO, strategi ST, dan strategi WT yang dapat digunakan dalam mempromosikan produk Maria Snack untuk meningkatkan penjualan, adalah sebagai berikut: Iklan yang digunakan seperti media sosial facebook, instagram, dan whatshapp yang dilakukan terus menerus dapat membuat merek produk Maria Snack lebih mudah diingat oleh masyarakat. Berhubungan baik dengan konsumen agar lebih dekat, sehingga akan terjadi penjualan dalam jangka panjang. Memanfaatkan teknologi dan media promosi agar mempermudahkan periklanan produk Maria Snack dan mengikuti event atau bazar yang diadakan. Meningkatkan hubungan dengan konsumen agar konsumen mepercayai produk Maria Snack
Beneficios de utilizar el Cuestionario para Evaluar las Disfuncionalidades de las Articulaciones Temporomandibulares en Artritis Reumatoide (CEDATAR)
Authors: Rhys Karen Vanesa , Gobbi Carla Andrea , Busamia Beatriz , Albiero Eduardo , Lagnarini Ana Laura , Alba Paula 
Published: December 2021

Han aumentado las técnicas para la examinación clínica e imagenológica de las articulaciones temporomandibulares, sin embargo, no sucede lo mismo en la posibilidad de evaluación en la anamnesis cotidiana en la consulta de rutina. Existen muy pocos cuestionarios y la mayoría son antiguos. Objetivo: Utilizar una herramienta útil y práctica para las interconsultas y derivaciones de pacientes entre el Servicio de Reumatología y el Servicio de Odontología. Material y métodos: Se implementó el Cuestionario para evaluar las disfuncionalidades de las articulaciones temporomandibulares en artritis reumatoide (CEDATAR) para evaluar si presentaba la capacidad de detectar alteraciones en las articulaciones que le sirvieran a los distintos profesionales en la decisión de derivar a un especialista al paciente. Resultados: Se evidenció que todos los pacientes que acusaron alteraciones en las respuestas del cuestionario, al corroborarse presentaron modificaciones importantes en el análisis imagenológico y/o clínico. Conclusiones: La artritis reumatoide es una enfermedad declarada de importancia en salud pública y políticas sanitarias, es de imponderable relevancia la utilización de CEDATAR en las consultas reumatológicas cotidianas para agilizar las terapéuticas limitando el avance de la enfermedad autoinmune a nivel de las articulaciones temporomandibulares suministrando un pronóstico más favorable y limitando lesiones y discapacidades.
Nomofobia: o telefone celular, o uso do tempo e o desengajamento ocupacional/Nomofobia: cell phone, time use and occupational disengagement
Authors: Cezar da Cruz Daniel Marinho , Bragatto Renata Lemes 
Published: December 2021

Objetivo: O ensaio fotográfico propõe, a partir do conceito de Nomofobia, refletir sobre o aspecto negativo da tecnologia móvel nas ocupações humanas. Descrição da imagem: É contextualizado brevemente o desenvolvimento da tecnologia de modo geral e suas diferentes consequências para os sujeitos na sociedade. A partir de quatro imagens ilustra-se a des-conexão com o mundo real, a contradição do estar em grupo, mas solitariamente, a dependência e o uso do tempo com a tecnologia móvel. Por fim, aponta-se a necessidade de pesquisas e de intervenções na terapia ocupacional, referindo-se à tecnologia móvel dentro do conceito de engajamento e desengajamento ocupacional. Palavras-chave: Telefone Celular. Ocupações Relacionadas com Saúde. Tempo de Tela. Terapia Ocupacional. AbstractObjective: From a photographic perspective, this essay proposes reflections based on the concept of Nomophobia, considering the negative side of mobile technology in human doings. Picture descripition: The development of technology in general and its different consequences for man in the society is briefly contextualized. Four photos illustrate the dis-connection with the real world, the contradiction of being in a group, but alone, the dependence and use of time with cell phones. Finally, the need for research and interventions in occupational therapy is pointed out, referring to mobile technology within the concept of occupational engagement and disengagement.Keywords: Cell Phone. Allied Health Occupations. Screen Time. Occupational Therapy. ResumenObjetivo: Desde una perspectiva fotográfica, este ensayo propone, desde el concepto de Nomofobia, considerar el lado negativo de la tecnología móvil en las actividades humanas. Descripción de la imagen: El desarrollo de la tecnología en general y sus diferentes consecuencias para el hombre se contextualiza brevemente. Cuatro fotos ilustran la desconexión con el mundo real, la contradicción de estar en un grupo, pero solo, la dependencia y el uso del tiempo con los teléfonos celulares. Finalmente, se señala la necesidad de investigación e intervenciones en terapia ocupacional, refiriéndose a la tecnología móvil dentro del concepto de involucración y desinvolucración ocupacional.Palabras clave: Teléfono Celular. Empleos Relacionados con Salud. Tiempo de Pantalla. Terapia Ocupacional.
Análise da prática de telessaúde em terapia ocupacional em um hospital universitário cardiológico/Analysis of the telehealth practice in occupational therapy in a university cardiological hospital
Authors: Chalegre Casiana Tertuliano , Melo Davy Leandro Leite 
Published: December 2021

Contextualização: Este relato tem como objetivo descrever o planejamento e os procedimentos adotados para a estruturação da prática da Terapia Ocupacional em Telessaúde com pacientes cardiopatas. Processo de intervenção: A atuação do terapeuta ocupacional na cardiologia busca planejar, prevenir e tratar as disfunções ocupacionais relacionadas às doenças cardiovasculares e que podem levar a deficiências, incapacidades e desvantagem social. Análise crítica da prática: O telemonitoramento em Terapia Ocupacional com estes pacientes está fundamentado no Modelo da Ocupação Humana e na abordagem centrada no cliente. Os objetivos chave foram traçados com base na avaliação terapêutica-ocupacional, que considerou interesses e ocupações desses indivíduos.Palavras-chave: Telemonitoramento. Terapia Ocupacional. Hospital Universitário. Cardiologia. AbstractContextualization: This report has the object to discribe the planning and the procedures adopted to structure the practice of Occupational Therapy in telehealth with cardiac patients. Intervention process: The occupational therapist’s performance in cardiology quests planning, preventing and treating the occupational disfunctions related to cardiovasvular diseases, which can lead to deficits, inability and social disadvantage. Practice analysis: The occupational therapy telehealth with these patients are based in the Model of Human Occupation with a client centered approach. The key objectives were planed through occupational therapy’s avaliation, which considered interests and occupations of the individuals.Keywords: Telemonitoring. Occupational Therapy. Universitary Hospital. Cardiology.ResumenContextualización: Este informe tiene como objetivo describir la planificación y los procedimientos adoptados para estructurar la práctica de la terapia ocupacional en telesalud con pacientes cardíacos. Proceso de intervención: El papel del terapeuta ocupacional en cardiología busca planificar, prevenir y tratar los trastornos ocupacionales relacionados con enfermedades cardiovasculares y que pueden conducir a discapacidades, discapacidades y desventajes sociales. Análisis crítico de la práctica: La telemonitorización en la terapia ocupacional con estos pacientes se basa en el modelo de ocupación humana y el enfoque centrado en el cliente. Los objetivos se elaboraron con base en la evaluación terapéutico-ocupacional, que consideró los intereses y ocupaciones de estos individuos.Palabras clave: Telemonitorización. Terapia ocupacional. Hospital Universitario. Cardiología.
Subsídios da perspectiva ocupacional para a abordagem ao desenvolvimento infantil/Subsidies from the occupational perspective for the approach to child development
Authors: Folha Débora Ribeiro da Silva Campos , Della Barba Patricia Carla de Souza 
Published: December 2021

Objetivo: Este manuscrito aborda a centralidade da compreensão das ocupações para a Terapia Ocupacional e objetiva apresentar a perspectiva ocupacional, enquanto subsídio para a compreensão e a atuação profissional dos terapeutas ocupacionais, junto à infância. Síntese dos elementos do estudo: Ancorado no conceito de desenvolvimento ocupacional, discutimos a perspectiva do desenvolvimento infantil, a partir e para o engajamento das crianças em ocupações. Conclusão: Acreditamos que a perspectiva ocupacional corresponde a um referencial de base para subsidiar as intervenções de terapeutas ocupacionais junto ao público infantil, na busca pelo fortalecimento da oferta de práticas e da produção de conhecimento, fundamentados nas bases e especificidades da Terapia Ocupacional.Palavras-chave: Terapia Ocupacional. Desenvolvimento infantil. Ocupações infantis. AbstractObjective: This manuscript addresses the centrality of understanding occupations for Occupational Therapy and aims to present the occupational perspective as a basis for understanding and the professional performance of occupational therapists in childhood. Synthesis of the study elements: Anchored in the concept of occupational development, we discuss the perspective of child development from and for the engagement of children in occupations. Conclusion: We believe that the occupational perspective corresponds to a basic reference to subsidize the interventions of occupational therapists with children, in the search for strengthening the offer of practices and the production of knowledge based on the bases and specificities of Occupational Therapy.Keywords: Occupational Therapy. Child development. Children's occupations ResumenObjetivo: Este manuscrito aborda la centralidad de la comprensión de las ocupaciones para la Terapia Ocupacional y tiene como objetivo presentar la perspectiva ocupacional como base para la comprensión y el desempeño profesional de los terapeutas ocupacionales en la infancia. Síntesis de los elementos de estudio: Anclado en el concepto de desarrollo ocupacional, discutimos la perspectiva del desarrollo infantil desde y para la participación de los niños en las ocupaciones. Conclusión: Creemos que la perspectiva ocupacional corresponde a un referente básico para subsidiar las intervenciones de los terapeutas ocupacionales con niños, en la búsqueda de fortalecer la oferta de prácticas y la producción de conocimiento a partir de las bases y especificidades de la Terapia Ocupacional.Palabras clave: Terapia Ocupacional. Desarollo infantil. Ocupaciones infantiles.