bluuebunny/crossref_metadata_2025_split huggingface dataset:
Establishment of an Antibody Specific for AMIGO2 Improves Immunohistochemical Evaluation of Liver Metastases and Clinical Outcomes in Patients with Colorectal Cancer
Authors: Goto Keisuke , Osaki Mitsuhiko , Izutsu Runa , Tanaka Hiroshi , Sasaki Ryo , Tanio Akimitsu , Satofuka Hiroyuki , Kazuki Yasuhiro , Yamamoto Manabu , Kugoh Hiroyuki , Ito Hisao , Oshimura Mitsuo , Fujiwara Yoshiyuki , Okada Futoshi 
Published: October 2021

Abstract
Instruction: The human amphoterin-induced gene and open reading frame (AMIGO) was identified as a novel cell adhesion molecule of type I transmembrane protein. AMIGO2 is one of three members of the AMIGO family (AMIGO1, 2, and 3), and the similarity between them is approximately 40% at the amino acid level. We have previously shown that AMIGO2 functions as a driver of liver metastasis. Immunohistochemical analysis of AMIGO2 expression in colorectal cancer (CRC) using a commercially available anti-AMIGO2 mouse monoclonal antibody clone sc-373699 (sc mAb) correlated with liver metastasis and poor prognosis. However, the sc mAb was found to be cross-reactive with all three molecules in the AMIGO family. Methods: We generated a rat monoclonal antibody clone rTNK1A0012 (rTNK mAb) for human AMIGO2. The rTNK mAb was used to re-evaluate the association between AMIGO2 expression and liver metastases/clinical outcomes using the same CRC tissue samples previously reported with sc mAb. Results: Western blot analysis revealed that a rTNK mAb was identified as being specific for AMIGO2 protein and did not cross-react with AMIGO1 and AMIGO3. The rTNK mAb and sc mAb showed higher AMIGO2 expression, which correlates with a high frequency of liver metastases (65.3% and 47.5%, respectively), while multivariate analysis showed that AMIGO2 expression was an independent prognostic factor for liver metastases (p = 7.930E-10 and p = 1.707E-5). The Kaplan-Meier analyses showed that the rTNK mAb (p = 0.004), but not sc mAb (p = 0.107), predicted worse overall survival in patients with high AMIGO2 expression. The relationship between AMIGO2 expression and poor disease-specific survival showed a higher level of significance for rTNK mAb (p = 0.00004) compared to sc mAb (p = 0.001). Conclusion: These results indicate that the developed rTNK1A0012 mAb is an antibody that specifically recognizes AMIGO2 by immunohistochemistry and can be a more reliable and applicable method for the diagnostic detection of liver metastases and worse prognosis in patients with high AMIGO2-expressing CRC.
Combination of rGO/S, N/TiO2 for the Enhancement of Visible Light-Driven Toluene Photocatalytic Degradation
Authors: Winayu Birgitta Narindri Rara , Mao Wan-Hua , Chu Hsin 
Published: October 2021

Abstract
Toluene is one type of common volatile organic compound (VOC) that is produced by daily products and is harmful to human health. Therefore, the degradation of toluene is critical to improving indoor air quality value. Photocatalytic degradation is considered an efficient and safe method to convert toluene into water and carbon dioxide without the formation of a secondary pollutant. Performance improvement of TiO2, a typically applied photocatalyst, has advantages in light absorption and electron transfer process. In this study, the TiO2 improvement was carried out by the doping of sulfur and nitrogen (S, N) elements along with various reduced graphene oxide (rGO). The composition of 0.1wt%rGO/S0.05N0.1TiO2 performed higher photocatalytic degradation of toluene due to the elevation of specific surface area, formation of oxygen-containing functional group, and chemical defect structure. However, a higher amount of rGO addition creates the shielding effect and inhibits the light penetration. Moreover, the relative humidity and applied temperature influence the photocatalytic activity through the competitive adsorption or increase the collisions frequency, respectively. During the photocatalytic degradation using 0.1wt%rGO/S0.05N0.1TiO2, toluene will be converted into benzyl alcohol, benzaldehyde, benzoic acid, water, and carbon dioxide.
Longitudinal evaluation of five nasopharyngeal carcinoma animal models on the microPET/MR platform
Authors: Shi Jingjing , Xue Zhichao , Tan Kel-Vin , Yuan Hui , Tsang Anna Chi Man , Tsao Sai Wah , Khong Pek-Lan 
Published: October 2021

Abstract
Purpose
We longitudinally evaluated the tumour growth and metabolic activity of two well-established nasopharyngeal carcinoma (NPC) animal models (C666-1, C17) and three novel models (Xeno76, Xeno23 and NPC43) using a microPET/MR system. With a better understanding of the interplay between tumour growth and metabolic characteristics of these NPC models, we aim to provide insights for the selection of appropriate NPC cell line/xenograft models to assist novel drug discovery and evaluation.
Methods
Mice were imaged by [18F]FDG microPET/MR twice a week for consecutive 3–7 weeks. [18F]FDG uptake was quantified by standardized uptake value (SUV) and presented as SUVmean tumour-to-liver ratio (SUVRmean). Longitudinal tumour growth patterns and metabolic patterns were recorded. SUVRmean and histological characteristics were compared across the five NPC models. Cisplatin was administrated to one selected optimal tumour model, C17 to evaluate our imaging platform.
Results
We found variable tumour growth and metabolic patterns across different NPC tumour types. C17 has an optimal growth rate and higher tumour metabolic activity compared with C666-1. C666-1 has a fast growth rate but is low in SUVRmean at endpoint due to necrosis as confirmed by H&E. NPC43 and Xeno76 have relatively slow growth rates and are low in SUVRmean, due to severe necrosis. Xeno23 has the slowest growth rate, and a relative high SUVRmean. Cisplatin showed the expected therapeutic effect in the C17 model in marked reduction of tumour size and metabolism.
Conclusion
Our study establishes an imaging platform that characterizes the growth and metabolic patterns of different NPC models, and the platform is well able to demonstrate drug treatment outcome supporting its use in novel drug discovery and evaluation for NPC.
Integration of Clinical and Transcriptomics Reveals Reprogramming of the Lipid Metabolism in Gastric Cancer
Authors: Li Yanyan , Zhao Jungang , Chen Renpin , Chen Shengwei , Xu Yilun , cai Weiyang 
Published: October 2021

Abstract
Background: Lipid metabolism has a profound impact on gastric cancer (GC) progression and is a newly targetable vulnerability for cancer therapy. Given the importance of lipids in cancer cellular processes, in this study we employed lipidomic clinical and transcriptomic data of GC to connect the variations of lipid metabolism changes.Method: We constructed a clinical nomogram based on the lipid factors and other clinical items. Then by using multi-omics techniques, we established a lipid-related gene signature for individualized prognosis prediction in patients with GC. Moreover, a total of 1357 GC cases were then applied to evaluate the robustness of this model. WGCNA was used to identify co-expression modules and enriched genes associated with GC lipid metabolism. The role of key genes ACLY in GC was further investigated.Results: The prognostic value of the lipidomic signature was analyzed using Cox regression model, and clinical nomogram was established. Among them, we observed overexpression of ACLY significantly increased the levels of intracellular free fatty acid and triglyceride, and activate AKT/mTOR pathway to promote cancer development.Conclusions: In conclusion, our findings delineated a GC clinical and lipidomic signature and revealed that GC exhibited a reprogramming of lipid metabolism in association with an altered expression of lipid metabolism-associated genes. Among them, ACLY significantly promoted GC lipid metabolism and increased cancer cell proliferation, suggesting that this pathway can be targetable as a metabolic vulnerability in future GC therapy.
Research-ready data for multi-cohort analyses: The Dementias Platform UK (DPUK) C-Surv data model
Authors: Bauermeister Sarah , Bauermeister Joshua R , Bridgman Ruth , Felici Caterina , Newbury Mark , North Laura , Orton Christopher , Squires Emma , Thompson Simon , Young Simon , Gallacher John E 
Published: October 2021

Abstract

Research-ready data (that curated to a defined standard) increases scientific opportunity and rigour by integrating the data environment. The development of research platforms has highlighted the value of research-ready data, particularly for multi-cohort analyses. Following user consultation, a standard data model (C-Surv), optimised for data discovery, was developed using data from 12 Dementias Platform UK (DPUK) population and clinical cohort studies. The model uses a four-tier nested structure based on 18 data themes selected according to user behaviour or technology. Standard variable naming conventions are applied to uniquely identify variables within the context of longitudinal studies. The data model was used to develop a harmonised dataset for 11 cohorts. This dataset populated the Cohort Explorer data discovery tool for assessing the feasibility of an analysis prior to making a data access request. It was concluded that developing and applying a standard data model (C-Surv) for research cohort data is feasible and useful.
The Comparison of Breast Cancer in Southern China and America: A Multicentre Study in China Versus SEER Database
Authors: Niu Yingjie , Zhang Fan , Chen Dong , Ye Guolin , Li Yong , Zha Yong , Chen Wenlin , Liu Dequan , Liao Xiaoming , Huang Qinghua , Tang Wei , Cai Gengxi , Guo Rong , Li Hongyang , Tang Shicong 
Published: October 2021

Abstract
Background and Objective: There are different characteristics of BC in developing countries and developed countries. We intend to study the factors which influence the survival and prognosis of BC between southern China and the United States. Methods: To study the two groups BC patients in southern China from 2001 to 2016 and SEER database from 1975 to 2016. To register, collect and analyze the clinicopathological features and treatment information. Results: Our study found that there are significant differences in tumor size, positive lymph node status and KI-67 between southern China and SEER cohort (P<0.000). Conclusions: The age, tumor size, positive-node and KI-67 may cause the difference of morbidity and mortality of BC patients in southern China and SEER cohort.
Successful treatment of a symptomatic unicameral calcaneus bone cyst by cement injection using a double needle technique under CT guidance: a case report
Authors: Abrishami Alireza , Zadeh Arezou Hashem , Ghanaati Hossein 
Published: October 2021

Abstract
Simple bone cysts (SBCs) are benign cavitary lesions which most commonly affect adolescent males in the first two decades of life. They are mainly asymptomatic, but can manifest with pain or pathological fractures. A 16-year old girl presented with an 8-day history of pain and swelling over the right calcaneal region. On local examination, tenderness was the only noteworthy sign and all routine laboratory tests conducted revealed normal results. Despite numerous proposed methods for the management of calcaneus SBCs, the optimal approach towards these lesions remains controversial. Here, a novel method utilizing a minimally invasive technique is proposed to successfully manage these lesions. In an outpatient setting, under conscious sedation, two interosseous needles were simultaneously inserted into the cyst under the guidance of CT fluoroscopy. Without aspiration, a radiopaque bone cement mixture was injected into the cyst from one needle until the cessation of serosanguineous fluid efflux from the second needle. Over a one-year follow-up period, the patient recovered without any complications. Bone cement injection using a double needle technique under CT fluoroscopy guidance is a feasible and safe method to treat symptomatic unicameral calcaneal bone cysts.
An Association Study Between VDR and HER2 in Breast Cancers Reveals the Potential of Combined Diagnosis and Prognosis
Authors: Dai Aimei , Han Ruilin , Chen Dongjing 
Published: October 2021

Abstract
Background: Breast cancer is generally acknowledged as a heterogeneous disease, with complete characterization and underlying technologies available to predict outcomes. Biomarkers, especially the human epidermal growth factor receptor 2 (HER2), are keys to characterize breast cancer and optimize the patients’ clinical outcomes. Vitamin D has an anti-cancer effect in breast cancer, which is related to the expression level of the vitamin D receptor (VDR). Despite these remarkable achievements, there is limited focus on the combined effects of VDR and HER2, which may provide specific diagnostic and prognostic potential in breast cancers. Results: In this study, we evaluated the association between VDR and HER2-status-related clinical prognostic characters by processing the breast cancer data of 2433 cases obtained from cBioPortal, discovering a high and positive correlation between VDR and HER2 in breast cancer. We also found substantial genes that are significantly correlated with both VDR and HER2 expressions. Those negatively correlated are enriched with pathways involving translations, showing a quite different mechanism from previous studies. Moreover, the survival analysis reveals that higher VDR copy number aberration (CNA) or HER2 expression shows significantly higher death rates in breast cancer patients.Conclusion: Our study reveals new mechanisms of coordinated function between VDR and HER2 in repressing cell translation, and prognosis or therapies targeted to VDR CNA and HER2 expression involving cell translations may be a potentially combined strategy in treating breast cancer.
Saliva As A Non-Invasive Specimen For COPD Assessment
Authors: Melo-Dias Sara , Valente Carla , Andrade Lília , Marques Alda , Sousa Ana 
Published: October 2021

Abstract
Background: People with COPD have been reported to bear a distinct airway microbiota from healthy individuals based on bronchoalveolar lavage (BAL) and sputum samples. Unfortunately, the collection of these samples involves invasive procedures preventing its regular use. Non-invasive samples from the upper airways could constitute an interesting alternative, but its relationship with COPD is still underexplored.We examined the merits of saliva to identify the typical profile of COPD microbiota and test its association with the disease.Methods: Outpatients with COPD and age-sex matched healthy controls were recruited and characterised based on clinical parameters and 16S rRNA profiling of salivary microbiota. A clustering analysis based on patients’ microbiota beta-diversity and logistic regressions were performed to evaluate the association between the microbiota composition and COPD. Results: 128 individuals participated (70 patients and 58 controls). Differential abundance analyses showed differences in patients comparable to the ones previously observed in samples from the lower respiratory tract, i.e., an increase in Proteobacteria (particularly Haemophilus) and loss of microbiota diversity. An unsupervised clustering analysis separated patients in two groups based on microbiota composition differing significantly in the frequency of patients hospitalized due to severe acute exacerbation of COPD (AECOPD) and in the frequency of GOLD D patients. Furthermore, a low frequency of Prevotella was associated with a significantly higher risk of recent severe AECOPD and of being GOLD D.Conclusion: Salivary microbiota showed an association with COPD, particularly with severe exacerbations, supporting the use of this non-invasive specimen for future studies of heterogeneous respiratory diseases like COPD.
Latent Myofascial Trigger Points Release Therapy for Adult Cough Variant Asthma
Authors: Liu Qianqian , Zhang Wenwen , Tian Tian , Bai He , Hu Qiya , Qi Feng 
Published: October 2021

Abstract
Background:Cough variant asthma (CVA) is a chronic inflammatory airway disease characterized by airway hyper-responsiveness (AHR). However, it’s precise mechanism is not clear yet. The date show that latent myofascial trigger points (MTrPs) can induce autonomic phenomena, such as over secretion, airway hyper-responsiveness, and so on. Therefore, we assumed that latent MTrPs might disrupt the balance between sympathetic and parasympathetic, which might play an essential role in the etiology of CVA.Results:Recurrence rate was lower in the intervention group than the control group (36w, 5.0% vs. 34.5%, P=0.001). There were significant between-group differences in change from baseline to 36 weeks in VAS (5.80±1.20 to 1.70±1.49 and 5.53±1.21 to 3.18±2.04, respectively; 95%CI, -2.00 to -1.00; P=0.001); ACT-scores (12.78±1.86 to 21.38±2.65 and 13.09±1.97 to 18.53±3.00, respectively; 95%CI, 2.00 to 4.00; P<0.001); ACQ5-scores (2.35±0.86 to 0.85±0.55 and 2.38±0.50 to 1.52±0.62, respectively; 95%CI, -1.00 to -0.40; P<0.001); AQLQ-scores (119.65±17.55 to 174.40±18.22 and 126.22±15.58 to 151.69±24.04, respectively; 95%CI, 14.00 to 34.00; P<0.001); Rate of rescue medication used (36w, 5.0% vs. 29.1%, P=0.003). Fewer adverse events were founded between the two groups (7.5% vs. 14.5%, P=0.462). Conclusions:Compared with budesonide-formoterol plus montelukast therapy, latent MTrPs injection therapy provided a long-acting, practical, short treatment course and safety methods for CVA. The findings indicated that latent MTrPs might play a vital role in the pathogenesis of CVA.Clinical Trials Registration: Chinese Clinical Trial Registry, ChiCTR2100044079. Registered 9 March 2021, http://www.chictr.org.cn/index.aspx
Fighting Amyotrophic Lateral Sclerosis: In-Silico Molecular Docking Study of SFPQ Protein to Identify a Potential Therapeutic Compound
Authors: Kovvali Omkar 
Published: October 2021

Abstract
Amyotrophic Lateral Sclerosis, known as ALS, is a neurodegenerative disease that passes the death sentence on 15 new people every day, leaving a patient struggling to move their fingers. At the molecular level, motor neuron death causes the inability for the muscles to function as normal. After researchers studied motor neuron death in ALS patients’ cells, they found that in all diseased cells, the SFPQ protein, a vital nuclear DNA/RNA binding protein, left the nucleus (its original location) and ended up in the cytoplasm. Since this defect was a molecular hallmark of ALS, solving this problem could stop motor neuron death. To treat this molecular hallmark, the in-silico process was used to sift through thousands of potential molecules to find the best one to use. The in-silico method was used to identify a lead molecule (NLS 551) that passed all the in-silico tests and brought the SFPQ protein back into the nucleus. If successful, this identified molecule could serve as the base for an ALS treatment.
Dietary Acid Load and Glomerular Filtration Rate in Chronic Kidney Disease
Authors: koor Behrooz ebrahimzadeh , Mousavizadeh Ali , PourAnsari Pardis , Jounaghani Milad Nasiri , Mohammadian Zahra , Khazaei Yasamin 
Published: October 2021

Abstract
Background: Kidney diseases are prevalencing rapidly. The nutritional transition has caused the diet of Iran community to increase the dietary acid load (DAL) and thus exacerbate metabolic disorders. Therefore, our goal was to determine the DAL of the diet in patients with Chronic Kidney Disease (CKD). Methods: In this cross-sectional study, the study population was composed of 90 patients with renal insufficiency. DAL was included of Potential Renal Acid Load(PRAL), Net EndogeneusAcid Production(NEAP) and Net Acid Excretion(NAE) that were extracted using data of food frequency questionnaire and their correlation with anthropometric and biochemical indices such as Glomerular Filtration Rate (GFR) and creatinine were analyzed by SPSS v.26 software with a significance level of < 0.05.Results: Mean of dietary acid load of participants were 17.15±9.85,-8.7±0.35 and 59.04±10.9 mEq/day for PRAL, NEAP and NAE respectively. Daily intake of energy (P<0.001) and protein percent of energy (P<0.01) in third tertile (T3) of PRAL were significant higher than first tertile (T1). Mean of age (P<0.05) and blood creatinine concentration (P<0.01) were significant higher in T1 than T1 of NEAP index and GFR was significant low (P<0.05). Daily energy intake (p<0.05), blood calcium concentration (p<0.05) and GFR (p<0.05) were significantly more in higher tertiles of NAE index. Based on Crude General linear model, the higher tertiles of PRAL compared to first tertile had significant lower GFR (P<0.05). In adjustment model, T3 group had more not significant GFR than T1 group. Only in model II adjustment, T2 group of NAE compared to T1, had higher GFR. Mean difference of GFR did not significant across tertiles of NEAP index. In case of Creatinine, based on model I adjustment, T3 group of NAE had more creatinine concentration than T1 group (P<0.01). T3 group of NEAP than T1 group had significant lower creatinine in crude and modelI adjustment (P<0.01). Discussion: Dietary Acid Load was associated with kidney function in CKD patients. In order to obtain logical results and to understand the cause-and-effect relationships, long-term studies with larger populations and consideration of blood factors such as blood bicarbonate are recommended.
LncRNA-KCNQ1OT1 Promotes the Odontoblastic Differentiation of Dental Pulp Stem Cells Via Regulating miR-153-3p/RUNX2 Axis
Authors: Lu Xiaohui , Zhang Jiawen , Lu Yuanzhou , Xing Jing , Lian Min , Feng Guijuan , Huang Dan , Wang Chenfei , Shen Nimei , feng xingmei 
Published: October 2021

Abstract
Background and Objective: Long non-coding RNAs (LncRNAs) play a key role in the odontoblastic differentiation. This study aimed to explore the role of LncRNA-KCNQ1OT1 in the odontoblastic differentiation of human dental pulp stem cells (DPSCs) and its possible mechanism. Methods: The expression of LncRNA-KCNQ1OT1, miR-153-3p, RUNX2 in the odontoblastic differentiation was detected by qRT-PCR. Interaction between LncRNA-KCNQ1OT1 and miR-153-3p and interaction between miR-153-3p and RUNX2 were detected by dual-luciferase assay. The cell viability of DPSCs was detected by cell counting kit-8 (CCK-8), and the effect of LncRNA-KCNQ1OT1 and miR-153-3p on the odontoblastic differentiation of DPSCs was observed by alizarin red staining, alkaline phosphatase (ALP) activity assay and Western blot for RUNX2, DSPP, DMP-1.Results: During odontoblastic differentiation of DPSCs, the expression of LncRNA-KCNQ1OT1 increased, miR-153-3p expression decreased, and RUNX2 expression increased. Dual-luciferase assay showed that LncRNA-KCNQ1OT1 sponges miR-153-3p and miR-153-3p targets on RUNX2. After LncRNA-KCNQ1OT1 and miR-153-3p expressions of DPSCs were changed, the cell viability was not notably changed, but the odontoblastic differentiation was notably changed which was confirmed with alizarin red staining, ALP activity and Western blot for RUNX2, DSPP, DMP-1.Conclusion: LncRNA-KCNQ1OT1 promotes the odontoblastic differentiation of DPSCs via regulating miR-153-3p/RUNX2 axis, which may provide a therapeutic clue for odontogenesis.