bluuebunny/crossref_metadata_2025_split huggingface dataset:
Influence of high heating rates on evolution of oxides on directed laser energy additively fabricated IN718
Authors: Mazumder Sangram , Pantawane Mangesh V. , Dahotre Narendra B. 
Published: September 2021

AbstractThe effect of non-isothermal treatment in oxygen-containing air, via heating rates of 10, 50, and 1000 °C/min until 1000 °C followed by furnace cooling to room temperature on oxides formed on directed laser energy additively fabricated IN718 was studied. Another set of samples heated up to 1000 °C using the same heating rates were isothermally held at 1000 °C for 1 hr followed by furnace cooling to room temperature. X-ray photoelectron spectroscopy indicated the presence of NiO on samples only heated at 1000 °C/min. Also, results indicated the absence of Fe-oxides on non-isothermally treated samples, irrespective of heating rate. However, isothermal treatment confirmed the presence of NiO on all samples and Fe-oxides on samples heated via 50 and 1000 °C/min. The durations in complement with the kinetics of the thermal treatments influenced oxide evolution in the samples. Such an experimental approach was adopted to study the material response under dynamic short duration-high temperature oxidation.
An artificial intelligence approach for selecting effective teacher communication strategies in autism education
Authors: Lampos Vasileios , Mintz Joseph , Qu Xiao 
Published: September 2021

AbstractEffective inclusive education is key in promoting the long-term outcomes of children with autism spectrum conditions (ASC). However, no concrete consensus exists to guide teacher-student interactions in the classroom. In this work, we explore the potential of artificial intelligence as an approach in autism education to assist teachers in effective practice in developing social and educational outcomes for children with ASC. We form a protocol to systematically capture such interactions, and conduct a statistical analysis to uncover basic patterns in the collected observations, including the longer-term effect of specific teacher communication strategies on student response. In addition, we deploy machine learning techniques to predict student response given the form of communication used by teachers under specific classroom conditions and in relation to specified student attributes. Our analysis, drawn on a sample of 5460 coded interactions between teachers and seven students, sheds light on the varying effectiveness of different communication strategies and demonstrates the potential of this approach in making a contribution to autism education.
Early life stress induces age-dependent epigenetic changes in p11 gene expression in male mice
Authors: Seo Mi Kyoung , Lee Jung Goo , Park Sung Woo 
Published: September 2021

AbstractEarly life stress (ELS) causes long-lasting changes in gene expression through epigenetic mechanisms. However, little is known about the effects of ELS in adulthood, specifically across different age groups. In this study, the epigenetic modifications of p11 expression in adult mice subjected to ELS were investigated in different stages of adulthood. Pups experienced maternal separation (MS) for 3 h daily from postnatal day 1 to 21. At young and middle adulthood, behavioral test, hippocampal p11 expression levels, and levels of histone acetylation and methylation and DNA methylation at the hippocampal p11 promoter were measured. Middle-aged, but not young adult, MS mice exhibited increased immobility time in the forced swimming test. Concurrent with reduced hippocampal p11 levels, mice in both age groups showed a decrease in histone acetylation (AcH3) and permissive histone methylation (H3K4me3) at the p11 promoter, as well as an increase in repressive histone methylation (H3K27me3). Moreover, our results showed that the expression, AcH3 and H3Kme3 levels of p11 gene in response to MS were reduced with age. DNA methylation analysis of the p11 promoter revealed increased CpG methylation in middle-aged MS mice only. The results highlight the age-dependent deleterious effects of ELS on the epigenetic modifications of p11 transcription.
Maternal age and maternal environment affect stress reactivity and measures of social behaviour in laying hens
Authors: Peixoto Mariana R. L. V. , Cooley Leanne , Widowski Tina M. 
Published: September 2021

AbstractMaternal effects can shape the phenotypes of offspring, but the extent to which a layer breeder’s experience can affect commercial laying hens remains unclear. We aimed to investigate the effects of maternal age and maternal environment on laying hens’ behaviour and stress response. In our first experiment (E1), commercial hybrid hens were reared either in aviary or barren brooding cages, then housed in aviary, conventional cages or furnished (enriched) cages, thus forming different maternal housing treatments. Hens from each treatment were inseminated at three ages, and measures of response to manual restraint and social stress were assessed in offspring. In experiment 2 (E2), maternal age effects on offsprings' stress response were further investigated using fertile eggs from commercial breeder flocks at three ages. In E1, maternal age affected struggling and corticosterone during manual restraint, feather pecking and pulling and comb wounds. Additionally, maternal rearing and housing in aviary systems showed positive effects on measures of behaviour and stress response in offspring. Effects of maternal age were not replicated in E2, possibly due to methodological differences or higher tolerance to maternal effects in commercial breeders. Overall, we recommend researchers report parent stock age to increase comparison across studies and thus our understanding of maternal age effects.
Microbiome of highly polluted coal mine drainage from Onyeama, Nigeria, and its potential for sequestrating toxic heavy metals
Authors: Oyetibo Ganiyu Oladunjoye , Enahoro Joy Aimiede , Ikwubuzo Chimuanya Amarachi , Ukwuoma Chiamaka Shileakanwa 
Published: September 2021

AbstractDrains from coal mines remain a worrisome point-source of toxic metal/metalloid pollutions to the surface- and ground-waters worldwide, requiring sustainable remediation strategies. Understanding the microbial community subtleties through microbiome and geochemical data can provide valuable information on the problem. Furthermore, the autochthonous microorganisms offer a potential means to remediate such contamination. The drains from Onyeama coal mine in Nigeria contained characteristic sulphates (313.0 ± 15.9 mg l−1), carbonate (253.0 ± 22.4 mg l−1), and nitrate (86.6 ± 41.0 mg l−1), having extreme tendencies to enrich receiving environments with extremely high pollution load index (3110 ± 942) for toxic metals/metalloid. The drains exerted severe degree of toxic metals/metalloid contamination (Degree of contamination: 3,400,000 ± 240,000) and consequent astronomically high ecological risks in the order: Lead > Cadmium > Arsenic > Nickel > Cobalt > Iron > Chromium. The microbiome of the drains revealed the dominance of Proteobacteria (50.8%) and Bacteroidetes (18.9%) among the bacterial community, whereas Ascomycota (60.8%) and Ciliophora (12.6%) dominated the eukaryotic community. A consortium of 7 autochthonous bacterial taxa exhibited excellent urease activities (≥ 253 µmol urea min−1) with subsequent stemming of acidic pH to > 8.2 and sequestration of toxic metals (approx. 100% efficiency) as precipitates (15.6 ± 0.92 mg ml−1). The drain is a point source for metals/metalloid pollution, and its bioremediation is achievable with the bacteria consortium.
Assessing the extent and timing of chemosensory impairments during COVID-19 pandemic
Authors: Cecchetto Cinzia , Di Pizio Antonella , Genovese Federica , Calcinoni Orietta , Macchi Alberto , Dunkel Andreas , Ohla Kathrin , Spinelli Sara , Farruggia Michael C. , Joseph Paule V. , Menini Anna , Cantone Elena , Dinnella Caterina , Cecchini Maria Paola , D’Errico Anna , Mucignat-Caretta Carla , Parma Valentina , Dibattista Michele 
Published: September 2021

AbstractChemosensory impairments have been established as a specific indicator of COVID-19. They affect most patients and may persist long past the resolution of respiratory symptoms, representing an unprecedented medical challenge. Since the SARS-CoV-2 pandemic started, we now know much more about smell, taste, and chemesthesis loss associated with COVID-19. However, the temporal dynamics and characteristics of recovery are still unknown. Here, capitalizing on data from the Global Consortium for Chemosensory Research (GCCR) crowdsourced survey, we assessed chemosensory abilities after the resolution of respiratory symptoms in participants diagnosed with COVID-19 during the first wave of the pandemic in Italy. This analysis led to the identification of two patterns of chemosensory recovery, partial and substantial, which were found to be associated with differential age, degrees of chemosensory loss, and regional patterns. Uncovering the self-reported phenomenology of recovery from smell, taste, and chemesthetic disorders is the first, yet essential step, to provide healthcare professionals with the tools to take purposeful and targeted action to address chemosensory disorders and their severe discomfort.
Delivery mode and perinatal antibiotics influence the predicted metabolic pathways of the gut microbiome
Authors: Vänni Petri , Tejesvi Mysore V. , Ainonen Sofia , Renko Marjo , Korpela Katja , Salo Jarmo , Paalanne Niko , Tapiainen Terhi 
Published: September 2021

AbstractDelivery mode and perinatal antibiotics influence gut microbiome composition in children. Most microbiome studies have used the sequencing of the bacterial 16S marker gene but have not reported the metabolic function of the gut microbiome, which may mediate biological effects on the host. Here, we used the PICRUSt2 bioinformatics tool to predict the functional profiles of the gut microbiome based on 16S sequencing in two child cohorts. Both Caesarean section and perinatal antibiotics markedly influenced the functional profiles of the gut microbiome at the age of 1 year. In machine learning analysis, bacterial fatty acid, phospholipid, and biotin biosynthesis were the most important pathways that differed according to delivery mode. Proteinogenic amino acid biosynthesis, carbohydrate degradation, pyrimidine deoxyribonucleotide and biotin biosynthesis were the most important pathways differing according to antibiotic exposure. Our study shows that both Caesarean section and perinatal antibiotics markedly influence the predicted metabolic profiles of the gut microbiome at the age of 1 year.
Serum markers change for intraocular metastasis in renal cell carcinoma
Authors: Sun Tie , Tang Jing , Pan Yi-Cong , Yu Chen-Yu , Li Biao , Zhang Li-Juan , Shu Hui-Ye , Ge Qian-Min , Shao Yi 
Published: September 2021

Abstract
Objective: Renal cell carcinoma is prone to early metastasis. In general, intraocular metastasis (IOM) is not common. In the present study, we studied the relationship between different biochemical indicators and the occurrence of IOM in renal cancer patients, and identified the potential risk factors.
Methods: A retrospective analysis of the clinical data of 214 patients with renal cell carcinoma from October 2001 to August 2016 was carried out. The difference and correlation of various indicators between the two groups with or without IOM was analyzed, and binary logistic regression analysis was used to explore the risk factors of IOM in renal cancer patients. The diagnostic value of each independent related factor was calculated according to the receiver operating curve (ROC).
Results: The level of neuron-specific enolase (NSE) in renal cell carcinoma patients with IOM was significantly higher than that in patients without IOM (P&lt;0.05). There was no significant difference in alkaline phosphatase (ALP), hemoglobin (Hb), serum calcium concentration, α fetoprotein (AFP), carcinoembryonic antigen (CEA), CA-125 etc. between IOM group and non-IOM (NIOM) group (P&gt;0.05). Binary logistic regression analysis showed that NSE was an independent risk factor for IOM in renal cell carcinoma patients (P&lt;0.05). ROC curve shows that the factor has high accuracy in predicting IOM, and the area under the curve (AUC) is 0.774. The cut-off value of NSE was 49.5 U/l, the sensitivity was 72.2% and the specificity was 80.1%.
Conclusion: NSE concentration is a risk factor for IOM in patients with renal cell cancer. If the concentration of NSE in the patient’s body is ≥49.5 U/l, disease monitoring and eye scans should be strengthened.
Intelligent control of a single‐link flexible manipulator using sliding modes and artificial neural networks
Authors: Lima Gabriel da Silva , Porto Diego Rolim , de Oliveira Adilson José , Bessa Wallace Moreira 
Published: September 2021

AbstractThis letter presents a new intelligent control scheme for the accurate trajectory tracking of flexible link manipulators. The proposed approach is mainly based on a sliding mode controller for underactuated systems with an embedded artificial neural network to deal with modelling inaccuracies. The adopted neural network only needs a single input and one hidden layer, which drastically reduces the computational complexity of the control law and allows its implementation in low‐power microcontrollers. Online learning, rather than supervised offline training, is chosen to allow the weights of the neural network to be adjusted in real time during the tracking. Therefore, the resulting controller is able to cope with the underactuating issues and to adapt itself by learning from experience, which grants the capacity to deal with plant dynamics properly. The boundedness and convergence properties of the tracking error are proved by evoking Barbalat's lemma in a Lyapunov‐like stability analysis. Experimental results obtained with a small single‐link flexible manipulator show the efficacy of the proposed control scheme, even in the presence of a high level of uncertainty and noisy signals.
Modeling the scattering polarization of the solar Ca I 4227 Å line with angle-dependent partial frequency redistribution
Authors: Janett Gioele , Ballester Ernest Alsina , Guerreiro Nuno , Riva Simone , Belluzzi Luca , del Pino Alemán Tanausú , Bueno Javier Trujillo 
Published: September 2021

Context. The correct modeling of the scattering polarization signals observed in several strong resonance lines requires taking partial frequency redistribution (PRD) phenomena into account. Modeling scattering polarization with PRD effects is very computationally demanding and the simplifying angle-averaged (AA) approximation is therefore commonly applied.
Aims. This work aims to assess the impact and the range of validity of the AA approximation with respect to the general angle-dependent (AD) treatment of PRD effects in the modeling of scattering polarization in strong resonance lines, with a focus on the solar Ca I 4227 Å line.
Methods. Spectral line polarization was modeled by solving the radiative transfer problem for polarized radiation, under nonlocal thermodynamic equilibrium conditions, taking PRD effects into account in static one-dimensional semi-empirical atmospheric models presenting arbitrary magnetic fields. The problem was solved through a two-step approach. In step 1, the problem was solved for the intensity only, considering a multilevel atom. In step 2, the problem was solved including polarization, considering a two-level atom with an unpolarized and infinitely sharp lower level, and fixing the lower level population calculated at step 1.
Results. The results for the Ca I 4227 Å line show a good agreement between the AA and AD calculations for the Q/I and U/I wings’ signals. However, AA calculations reveal an artificial trough in the line-core peak of the linear polarization profiles, whereas AD calculations show a sharper peak in agreement with the observations.
Conclusions. An AD treatment of PRD effects is essential to correctly model the line-core peak of the scattering polarization signal of the Ca I 4227 Å line. By contrast, in the considered static case, the AA approximation seems to be suitable to model the wing scattering polarization lobes and their magnetic sensitivity through magneto-optical effects.
In vitro anthelmintic activity of an aqueous extract of Glycyrrhiza glabra and of glycyrrhetinic acid against gastrointestinal nematodes of small ruminants
Authors: Maestrini Michela , Molento Marcelo Beltrão , Forzan Mario , Perrucci Stefania 
Published: September 2021

This study evaluated the in vitro anthelmintic activity of a liquorice (Glycyrrhiza glabra) root aqueous extract and of glycyrrhetinic acid at 30, 10, 5, 1, and 0.5 mg/mL against sheep gastrointestinal nematodes (GINs), using the egg hatch test (EHT), the larval development test (LDT), and the larval migration inhibition test (LMIT). The compounds were applied on a mixture of GIN eggs and larvae, mainly Trichostrongylus spp. and Teladorsagia/Ostertagia spp. Cytotoxicity assays were also performed. In the EHT, both candidates showed significant concentration-dependent efficacy and were significantly more effective (p < 0.001) at the highest concentrations (30 and 10 mg/mL) than the lowest ones. In the LDT, only G. glabra showed a concentration-dependent effect (R2 = 0.924), but glycyrrhetinic acid (R2 = 0.910) had significantly higher efficacy than G. glabra root extract. Moreover, the efficacy of glycyrrhetinic acid at 30, 10, and 5 mg/mL was significantly higher (p < 0.001) than at lower concentrations. In the LMIT, G. glabra showed concentration-dependent efficacy (R2 = 0.971), while considerably reduced efficacy was observed for glycyrrhetinic acid (R2 = 0.855) at the lowest concentrations. These data suggest that the two compounds may have different mechanisms of action. In the LMIT, the 50% lethal concentration (LC50) of glycyrrhetinic acid (~5.12 mg/mL) was > 2.0-fold lower when compared to G. glabra (12.25 mg/mL). Analysis and previous findings indicated low toxicity for both compounds. The results obtained encourage in vivo studies aimed at evaluating the potential use of the tested compounds as natural de-wormers in ruminants.
Group-Assisted-Purification Chemistry Strategy for the Efficient Assembly of Cyclic Fused Pyridinones
Authors: Chen Wenteng , Ke Di , Wu You , Zhang Lei , Shao Jiaan , Yu Yongping 
Published: September 2021

AbstractA group-assisted-purification (GAP) chemistry strategy-based Ugi four-center three-component reaction (Ugi-4C-3CR) was explored. The reaction proceeded well to deliver the cyclic fused pyridinones selectively. Moreover, the reaction conditions were mild and avoided additional chromatography or recrystallization workup. Also, wide variations in substrates, such as anilines and aliphatic amines as well as amino alcohols and amino acid esters were all tolerated and pyridinones are achieved in good to excellent yields. Importantly, ladder-type cyclic fused pyridinones can be further constructed in excellent yield of 91%.
Dihydroxylation Studies of Isoquinolinones: Synthesis of the EF-Ring of Lysolipin I
Authors: Menche Dirk , Heinemann Maximilian J. B. , Voigt Thomas 
Published: September 2021

AbstractInspired by the potent polycyclic xanthone antibiotic lysolipin I, a general study on asymmetric dihydroxylation reactions of variously substituted isoquinolinones was performed. Different isoquinolinones were efficiently prepared, either by a Pomeranz–Fritsch type condensation or a Curtius rearrangement. Under a broad variety of conventional oxygenation procedures, they proved very unreactive. However, either by suitable substitution of the appending aromatic ring or more forcing conditions a dihydroxylation could finally be performed, which allowed the synthesis of the EF-ring of lysolipin I.
Evidence for Reversal of Immunosuppression by Homeopathic Medicine to a Predominant Th1-type Immune Response in BALB/c Mice Infected with Leishmania donovani
Authors: Joshi Jyoti , Bandral Chetna , Manchanda Raj Kumar , Khurana Anil , Nayak Debadatta , Kaur Sukhbir 
Published: August 2021

Abstract
Background Visceral leishmaniasis (VL) is a neglected tropical disease that is fatal if treatment is not given. The available chemotherapeutic options are unsatisfactory, and so complementary therapies like homeopathy might be a promising approach.
Methods A nosode from a pure axenic culture of Leishmania donovani was prepared and screened for its anti-leishmanial potential both in an in-vitro and an in-vivo experimental approach.
Results Leishmania donovani amastigote promastigote nosode (LdAPN 30C) exhibited significant anti-leishmanial activity against the promastigote forms of Leishmania donovani and was found to be safe. A study conducted on VL-infected mice revealed that LdAPN 30C resolved the disease by modulating the host immune response toward the Th1 type through upregulating the pro-inflammatory cytokines (IFN-γ and IL-17) and inducing nitric oxide (NO) levels in the infected macrophages. The hepatic parasite load was also found to be significantly decreased. The nosode was found to be safe, as no histological alterations in the liver or kidney were observed in the animals treated with the LdAPN 30C.
Conclusion This is the first study in which an axenic culture of Leishmania donovani has been used for the preparation of a homeopathic medication. The study highlights the anti-leishmanial and immunomodulatory potential of a homeopathic nosode in experimental VL.
Free space Thomson scattering to study high energy density shocks
Authors: Banasek J. T. , Oliver T. G. , Cordaro S. W. , Bott-Suzuki S. C. 
Published: September 2021

A free space collective Thomson scattering system has been developed to study pulsed power produced plasmas. While most Thomson scattering diagnostics on pulsed power machines use a bundle of fibers to couple scattered light from the plasma to the spectrometer, this system used free space coupling of the light, which enabled a spatially continuous image of the plasma. Initial experiments with this diagnostic were performed on an inverse wire array generated by a 200 kA, 1100 ns rise time pulse power generator. The capabilities of this diagnostic were demonstrated by using the low frequency ion acoustic wave feature of the Thomson scattering spectra to measure the plasma flow velocity. The diagnostic was demonstrated to measure velocities between 20 and 40 km/s with an error of 4.7 km/s when fitting with a 600 μm spatial resolution or 8.9 km/s when fitting with a 150 μm spatial resolution. In some experiments, the diagnostic observed a bow shock in the plasma flow as the scattering intensity increased and flow velocity decreased.