bluuebunny/crossref_metadata_2025_split huggingface dataset:
SENSITIVITY OF SWINE CORONAVIRUS TO ACTION OF DIFFERENT TEMPERATURES
Authors: Klestova Z. S. 
Published: June 2021

Coronavirus infections are currently receiving a lot of attention due to the emergence of the covid-19 pandemic in the world. The search for models with which it is possible to obtain an adequate result to identify the properties of coronaviruses is intensive. For comparison with the results of our experiments, the article also presents data on the duration of preservation of the infectious properties of the SARS-Cov-2 virus by contamination of surfaces made of different materials and at different temperatures.
We studied the long-term effect on coronavirus, the causative agent of transmissible swine gastroenteritis (TGE), a number of temperatures: + 4ºC, + 25ºC, minus 13ºC, minus 20ºC and thirteen-fold change in temperature, which was in the range of 31-33ºC. It was found that both vaccine and epizootic strains of TGE coronavirus after long-term storage reduce the infectious properties, but when in contact with a sensitive biological system (in vitro) quickly enough (in the case of successive passages in this system) restore them. We proved that the TGE coronavirus during storage for more than two years reduced, but did not lose infectious properties at temperatures minus 13ºC, minus 20ºC, which were restored during subsequent passage in sensitive biological systems in vitro. The same trend was observed for storage of the virus for 8 years at a temperature of + 4ºC. The fastest decrease in coronavirus titer occurred at a temperature of + 25ºC, but more stable under these conditions was the epizootic strain of the virus, which requires attention when working with field isolates of coronavirus.
The general tendency of animal and human coronaviruses to decrease the survival time of the pathogen with increasing exposure temperature has been established. Resistance of the virus to repeated sharp changes in temperature (from minus 13 ± 0.5°C to room temperature) without loss of infectious properties was revealed.
INVESTIGATION OF DIAZINON DEGRADATION IN MODEL SOLUTIONS USING HIGH PERFORMANCE LIQUID CHROMATOGRAPHY
Authors: Korobova O. V. , Samarska L. K. , Humenjuk S. L. , Bereziyk M. I. , Yurkevych M. V. 
Published: June 2021

Aim of our war was to investigate the degradation of diazinon in the model mixture in comparison with the experimental preparation. Methyl alcohol was used as a basis for the manufacture of diazinon drops and the model mixture. The concentration of diazinon was determined by reversed-phase high performance liquid chromatography on the third, fourteenth and twenty-first day after preparation of the model mixture and experimental preparation when stored at room temperature.
Despite the large number of publications studying the degradation of diazinon under the influence of various factors, there is a need to study this process in mixtures of a specific composition, in particular in new veterinary drugs that have not yet been studied. This article presents the results of determining the content of diazinon in 10% of the drug and the model mixture based on methyl alcohol.
The analyzes were performed on a Knauer liquid chromatograph with a spectrophotometric detector equipped with a Luna® Omega Polar C 18 150 × 4.6 mm column filled with a 5 μm particle sorbent from Phenomenex with a universal C 18 pre-column 4 × 3.0 mm ID. Mobile phase: degassed mixture of acetonitrile: water in the ratio 65:35, flow rate was 1.1 ml/min, temperature of the column was ambient. Diazinone was detected at 245 nm. Injection volume was 0.050 ml and the time of one separation – 20 minutes.
The appearance of an unknown peak on the 3 min chromatogram of the solution of the production experimental preparation was revealed. On the twenty-first day of storage of the experimental drug, the content of diazinon decreased to 34 % of the stated amount. On the third and twenty-first day of storage of the model mixture, a decrease in the content of diazinon by 16 % and 79%, respectively, and the appearance of an additional peak were observed.
The use of methyl alcohol with other components is likely to be a factor in the breakdown of diazinon in the drug. According to the recipe of the drug, methanol is about half the content of ingredients. This composition does not provide stability of the proposed dosage form of the drug. The next step will be to study the effect of other solvents on maintaining the stated concentration of diazinon.
СHARACTERISTICS OF DIETARY FEEDS FOR DOMESTIC ANIMALS AVAILABLE ON THE MARKET OF UKRAINE
Authors: Kosenko Yu. M. , Vezdenko O. S. , Zaruma L. Ye. , Sekh O. A. , Shkilnyk O. S. 
Published: June 2021

Complete and complementary dietary feeds are feeds intended for special nutritional purposes and used when the nutritional needs of the animal's body are met. The labeling of feeds intended for special nutritional purposes must contain the word "dietary". Their composition is balanced by proteins, fats, carbohydrates, vitamins, minerals, pre- and probiotics, and other pharmacologically active components that affect the digestive processes, excretion or prevention of kidney stones, strengthening joint tissue, suspending allergic reactions, renal dysfunction, liver, promote recovery from stress, etc.
The article describes the characteristics of feeds intended for special nutritional purposes for pets, that are imported or the domestic products, which are authorized in Ukraine for the period 2015-2019. The names of finished feed products, in addition to trademarks, reflect the animal species and purpose, depending on the identified abnormalities in their health, which determines the special purpose for their use.
According to the authorized products list, the largest number of names falls on dietary foods for pets used in kidney disease and kidney stones (70), digestive disorders (51) and allergies (37). A significant number (28) are low-calorie diets for overweight cats and dogs. The share occupied by certain groups of complete feeds by purpose meets the needs of the domestic market of complete dietary feeds for pets. This distribution of feeds intended for special nutritional purposes helps to ensure proper care for pets of different ages and breeds that are prone to disease. Feeds entering the market in Ukraine meet the requirements of national regulations and the main safety criteria of the European Federation of Animal Feed Manufacturers (FEDIAF) and the ISO standard 22 000: 2005.
According to scientific research, the most common groups of feeds intended for special nutritional purposes for pets are described; the characteristics of the main feed ingredients in the formulas, and substantiated their function as a part of nutrition at special nutritional purposes of the animal's body.
ACTIVITY AND CONTENT OF ISOZYMES OF ASPARTATE AMINOTRANSFERASE IN CONNECTION WITH THE SURVIVAL OF BULL SPERMATOZOA
Authors: Kuzmina N. V. , Ostapiv D. D. , Chajkovska O. I. , Ostapiv R. D. , Panych O. P. 
Published: June 2021

The activity and content of aspartate aminotransferase (AST) isoforms in bovine ejaculates due to sperm survival were studied.
Ejaculates of bulls of the Ukrainian black-spotted dairy breed (n = 22) were selected for research. In freshly obtained and incubated semen the activity and isozymes of AST was studied in connection with spermatozoa survival at a temperature of 2-4 ° C (on the first, second, third and fourth days) until the cessation of rectilinear translational movement.
AST activity depended on the duration of sperm survival. When survival was more than 100 hours - AST activity was the highest - 65.2 ± 1.7 nmol / min × mg of protein. When survival was lower - up to 100 hours, enzymatic activity lower by 26.8% (P <0.001). Two enzymes of the enzyme (AST1 and AST2) were found in the semen of the fetuses, which differ in electrophoretic mobility and intensity of staining in 7.5% polyacrylamide gel.
The established correlation with sperm survival time has a strong straight line for AST1 (η2АSТ1 = 0.88) and inverse - for AST2 (η2АSТ2 = 0.87) isozymes. During sperm incubation, the ratio of AST isozymes changes - the content of AST1 increases and decreases - AST2. The correlation ratio for sperm survival for enzyme activity and isozymes is up to 100 hours, respectively. - η2АSТ = 0.83; η2АSТ1 = 0.68 and η2АSТ2= 0.69 and more than 100 hours - η2АSТ = 0.75; η2АSТ1= 0.92 and η2АSТ2= 0.69.
Therefore, ejaculates of bulls with reduced sperm survival are characterized by lower AST activity and, accordingly, the speed of the amino acid transamination process. Increased supply of substrates from the cytosol in the mitochondria of germ cells ensures high survival of sperm. Changes in the activity and content of AST isozymes, which characterize the energy supply of germ cells, can serve as a criterion for the physiological quality of sperm of freshly obtained sperm.
THE INFLUENCE OF POLYHEXAMETHYLENE GUANIDINE SALTS ON THE MICROBIOLOGICAL PARAMETERS OF WATER
Authors: Kushnir I. M. , Kolodiy G. V. , Kushnir V. I. , Murska S. D. , Semen I. S. , Berbeka U. Z. 
Published: June 2021

The article represent the use of disinfectant which was made based on salts of polyhexamethylene guanidine hydrochloride (PGMG-hydrochloride) and polyhexamethylene guanidine phosphate (PGMG-phosphate) for water disinfection. Water plays one of the most important roles in almost all life processes. Water is a part of the blood, participates in blood circulatory system, delivers nutrients and oxygen to the organs, participates in oxidation reaction, hydrolysis and other reactions of intercellular metabolism, forms an environment to maintain a healthy microflora of the digestive tract, which provides effective breakdown of nutrients by the optimal amount of enzymes.
Good drinking water quality must to be epidemically safe and free of pathogenic microbes, viruses and other biological inclusions. Also must be harmless in chemical composition, have good organoleptic properties ‒ be transparent, colorless, have no taste or odor.
Water quality is assessed by total microbial contamination and the amount of bacteria of the Escherichia coli group in 1 cm3 of water, and also by thermostable Escherichia coli (fecal coliforms) in 100 cm3 of water, pathogenic microorganisms and the amount of coli-phages.
In result of the conducted researches it was found that the use of disinfectant in 0,2 % concentration did not reduce the total microbial pollution of water. In particular, the efficiency of 1, 2 and 3-hour of exposure was 57, 62 and 68 %, respectively.
After application of disinfectant in 0,3 % concentration the efficiency of its application was for 1 h – 69 %, 2 h – 82 %, 3 h – 100 %.
It was found that the use of a disinfectant, which was made based on PGMG salts in 0,4 % concentration contributed to reduce the total microbial contamination of water. The efficiency by the 1, 2 and 3 hour of exposure was, respectively, 73, 86 and 100 %.
Therefore, the use of a disinfectant, containing in its composition PGMG salts, in 0,3% concentration at 3 hours of exposure was more optimal, which contributed to reduce the total microbial contamination of water.
ASSESSMENT OF FEED SUPPLEMENT SAFETY FOR CHICKEN BROILERS
Authors: Levytskyy T. R. , Boiko H. I. , Vilkha O. M. , Nedilka H. Ju. , Fedor H. Ju. 
Published: June 2021

A study of the safety of the use of feed additive Liaform for the target species – broiler chickens.
Liaform is a yellowish liquid with a specific odor. The feed additive contains lactic acid, formic acid, sodium formate, propionic acid and water. Given the composition of the additive, it can be attributed to two functional groups: preservatives – substances or microorganisms that protect feed from damage by microorganisms or their metabolites, acidity regulators – substances that regulate the pH of feed.
For the study was formed on the principle of analogues of 3 groups of broiler chickens of 15 heads (control and 2 experimental) from 8 days of age. Feeding of chickens of the control group was carried out by standard complete feed. Feeding of chickens of the first experimental group was carried out with standard complete feed, in which the feed additive Liaform was added at the rate of 2 g / kg, feeding of chickens of the second experimental group was carried out with standard complete feed, in which feed additive Liaform was applied in 10 times the recommended dose of 20 g/kg. During the experiment, the general condition of the bird, the safety of the bird, zootechnical and hematological parameters were monitored.
It was found that the studied feed additive in 10 times the recommended dose does not have a negative impact on productivity, growth and development of broiler chickens, feed absorption. As a result of the use of Liaform feed additive in 10 times the recommended dose, the live weight of broiler chickens in the experimental group was higher compared to the control and lower compared to the recommended dose. The use of a feed additive in 10 times the dose did not have a negative effect on hematological parameters and serum protein levels, did not cause a negative effect on the condition of internal organs.
SOME QUALITY INDICATORS OF VEGETABLE OILS UNDER DIFFERENT STORAGE CONDITIONS
Authors: Levytskyy T. R. , Moravskaya O. V. , Fedor G. Yu. , Nedilka G. Yu. , Kushnir G. V. 
Published: June 2021

The aim of our work was to study the quality of vegetable oils - unrefined sunflower oil (grade I) and soy hydrated oil (grade I). One of the stages of the study was to determine the quality of vegetable oils - peroxide value (characterizes the amount of primary oxidation products of fats - peroxide compounds) and acid number (characterizes the total content of free fatty acids) in the samples of vegetable oils. The acid number was determined by titration (neutralization) of free fatty acids with alkali in the presence of an indicator (phenolphthalein). The peroxide number was determined by titration of the isolated iodine with a solution of sodium thiosulfate. Each study was reproduced 5 times. First of all, the quality indicators of oils (acid number and peroxide number) were determined in samples of fresh oils. Subsequently, the oil was kept for 3 months (in compliance with the recommended requirements for storage of oils and without compliance with the requirements for storage of oils) and re-determined these indicators.
The quality of the investigated vegetable oils was determined by the indicators of acid number and peroxide number in accordance with the established requirements of the State Standards of Ukraine (DSTU EN ISO 660: 2009; DSTU 4570: 2006).
The results of our research show that the level of acid number and peroxide level in samples of fresh oils meet the requirements of the State Standards of Ukraine (DSTU EN ISO 660: 2009; DSTU 4570: 2006) for this type of oil, which confirms the quality of oils and compliance with production , processing and transportation. It is shown that in the samples of aged oils (3 months, subject to storage requirements) the level of acid number and the level of peroxide number increases within normal limits. However, in the samples of aged oils (3 months, without compliance with storage requirements) there is a significant increase in the level of acid number and peroxide number, which does not meet the requirements of State Standards of Ukraine.
As a result of research it is shown that the quality of vegetable oils depends on compliance with the recommended norms and conditions in the process of production, processing, transportation and storage. It is proved that under the conditions of violation of the recommended norms of storage in samples of high-quality vegetable oils the level of quality indicators of vegetable oils - acid number and peroxide number significantly increases.
COMPREHENSIVE EVALUATION OF SOYBEAN AND SUNFLOWER PROCESSING PRODUCTS
Authors: Ryvak Н. P. , Boyko G. I. , Ryvak R. O. 
Published: June 2021

The literature review of the article presents the characteristics of the protein value of plant feed materials, in particular, of soybean and sunflower processing products, depending on the production technology. Their amino acid composition ideally meets the needs of young animals and poultry due to the high content of essential amino acids.
The section «Materials and methods» presents methods for determining: crude protein by the Kjeldahl method, soluble protein in 0.2% potassium hydroxide, Barnstein protein, falsification of protein products with inorganic nitrogen, as well as characteristics of methods for determining the content of essential and non-essential amino acids, incl. sulfur-containing, using the system of capillary electrophoresis «Drops-105M».
As a result of the research it was found that of the total number of analyzed samples of soybean meal in terms of protein content, 30.8% of products did not meet the declared values of producers and were in the range of 37.4 - 40.8%, in terms of protein solubility were in the range of 75 - 78%, which meets the norms of feeding.In addition, 5.0% of the total number of samples analyzed, were falsified after Bard alcohol, falsification of inorganic nitrogen was not found. The use of low-protein raw materials for the production of sunflower meal and cake was reflected in their protein content and ranged from 26.3 to 33.7%.
Fermented soybean product and toasted soybean meal are characterized by a higher total content of essential amino acids in the protein, which was 26.3% and 27.2%, respectively, compared with full-fat expanded soybean expanded, where this figure was at 20.25%, and prevailed other analyzed products by methionine, threonine, tyrosine, phenylalanine, proline, alanine etc.
The analyzed sample of sunflower meal, produced by a special technology is equivalent in total amino acid content in the studied soy products. Its content of methionine was 1.5 and 2.1 times higher, compared with toasted soybean meal and full-fat soybean, respectively. Also, the content of threonine, glycine, cystine, tryptophan, aspartic and glutamic acids in the sample of sunflower meal prevailed the content of the same amino acids in soybean products.
Thus, the comprehensive assessment of plant protein components makes it possible to control not only the quantitative content of protein and amino acids, but also provides a detailed assessment of feeding.
FATTENING AND MEAT QUALITIES OF GEESE OF OBROSHIN SELECTION WITH GRAY AND WHITE PLUMAGE
Authors: Ferenc L. V. , Petriv M. D. 
Published: June 2021

The research work provided a solution to the problem of preserving the gene pool of Obroshyn gray (OS) and Obroshyn white (OB) geese, which will improve and enhance the valuable qualities of the original flock of geese, gain the desired economic characteristics and thus ensure the competitiveness of the industry in modern conditions.
The research was conducted in the laboratory of small livestock ISGKR NAAS and on the basis of SE DG "Miklashiv".
The main method of breeding is the selection and selection of individuals with high productive qualities in order to obtain the same type of bird that would meet the planned performance parameters.
Selection work was carried out by individual mass selection, aimed at consolidating the standard for each breed group of traits. Before the beginning of the breeding period, males and females of both groups were individually assessed by the exterior, typical plumage, live weight.
It was found that both Obroshyn gray and Obroshyn white geese were well selected for productive characteristics and showed high fattening and meat qualities. All birds are well adapted to the natural conditions of the western region.
Body sex measurements (chest circumference, torso length, keel, and metatarsus) were higher in males of the Obroshyn gray breed group of geese. Males at different ages had higher rates than females.
Obroshyn gray geese (group I) outperformed their peers from group II in terms of meat qualities, the weight of uncorked and gutted carcass, and yield of edible parts.
The pre-slaughter live weight of carcasses of males of group I (OS) was 4722 g and prevailed over peers of group II (OB) by 2.94%, and the live weight of females was 4149 g and was higher by 4.19%.
It was found that the difference in the number of erythrocytes in males and females was insignificant and almost does not change with age, and hemoglobin on the contrary - increases with age. Males in all study periods had higher serum total protein compared to females. The lowest rate was in males and females аt 4 weeks of age, however, there was no statistically significant difference between the groups in the number of erythrocytes, hemoglobin, and total protein content.
THE DEVELOPMENT AND THE VALIDATION OF THE METHOD FOR TRIMETOPRIM RESIDUES DETERMINATION IN TISSUES SAMPLES USING THE METHOD OF ENZYME-LINKED IMMUNOSORBENT ASSAY
Authors: Yanovych D. , Zasadna Z. , Rydchuk M. , Plotycya S. , Kislova S. , Pazderska O. 
Published: June 2021

The article presents the results of the development of sample preparation method of muscle and parenchymal tissues for their further testing on trimethoprim residues by enzyme-linked immunosorbent assay using Trimethoprim test kits from Kwinbon Biotechnology (China), designed to analyze another target matrix, viz. honey. The results of the comparison of sample preparation methods with the use of different techniques of analyte extraction from tissues, with the established percentage of extraction are presented. The most effective conditions are proposed: acetonitrile extraction using ultrasonic intensification, subsequent concentration of the analyte by evaporation, reconstitution of dried residue and degreasing. This approach makes it possible to determine trimethoprim residues in poultry tissues at the level of 5-10 μg/kg with an absolute recovery of about 75%. In accordance with the Decision of the European Commission 2002/657/EC, the validation of the proposed method was carried out taking into account the maximum permissible levels (MRL) of trimethoprim in the tissues of productive animals. The suitability of the method was confirmed on the basis of establishing of the main validation parameters for screening methods (technical threshold and cut-off factor) using control (pure) and spiked with a standard solution of trimethoprim at the level of ½ MRL samples of muscle and parenchymal tissues of broiler chickens. The main advantages of the developed technique are simplicity of performance, rapidity and cost effectiveness. The certainty of the results obtained using the developed method was verified using the confirmatory UPLC-MS/MS method. The article presents the results of a comparative study of pure samples of broiler chickens muscles and parenchymal tissues and spiked at the level of ½ MRL with a standard solution of trimethoprim. The developed screening technique of enzyme-linked immunosorbent assay is recommended for determination of residual amounts of trimethoprim in tissue samples and can be used for routine laboratory tests, as well as to study the withdrawal procedure of veterinary drugs based on this drug.