bluuebunny/crossref_metadata_2025_split huggingface dataset:
Probiotics or Synbiotics Addition in Bama Mini-pigs’ Diets Improves Growth Performance, Carcass Traits, and Meat Quality by Altering Plasma Metabolites and Related Gene Expression of Offspring Pigs
Authors: Zhu Qian , Song Mingtong , Azad Md. Abul Kalam , Ma Cui , Yin Yulong , Kong Xiangfeng 
Published: October 2021

Abstract
Background: The present study evaluated the effects of maternal probiotics or synbiotics addition on growth performance, carcass traits, meat quality, plasma metabolites, and related gene expression of their offspring pigs. Methods: A total of 64 Bama mini-pigs were used and randomly divided into the control, antibiotic, probiotics, or synbiotics groups, and fed experimental diets during pregnancy and lactation. After weaning, two piglets per litter and eight piglets per group were selected and fed a basal diet. At 65-, 95-, and 125-day-old, eight pigs per group were selected for analysis. Results: The results showed that probiotics addition increased the ADFI of pigs during the 66-95 day-old and the backfat thickness at 65- and 125-day-old, whereas synbiotics addition increased backfat thickness and decreased muscle percentage and loin-eye area at 125-day-old. In meat quality, probiotics or synbiotics addition increased cooking yield, pH 45min , and meat color, while decreased drip loss and shear force at different stages. However, maternal antibiotic addition showed an increased shear force at 125-day-old. Dietary probiotics or synbiotics addition in sow diets increased several amino acids (AA) contents in the plasma and longissimus thoracis (LT) muscle, including total AA (TAA), His, Met, Asp, Arg, and Leu, while decreased Gly, Pro, Ile, α-AAA, α-ABA, β-Ala, and γ-ABA at different stages. In the LT muscle fatty acid (FA) analysis, the contents of saturated FA (SFA; including C16:0, C17:0, and C20:0) and C18:1n9t were decreased, and the contents of C18:2n6c, MUFA (C16:1 and C20:1) and UFA were increased in the probiotics group, while the contents of SFA (C10:0, C12:0, and C14:0 at 65-day-old) were increased and the contents of C20:1 and C18:1n9t were decreased in the synbiotics group. Plasma biochemical analysis revealed that maternal probiotics or synbiotics addition decreased plasma AMM, UN, and GLU, while synbiotics addition increased plasma ALT, HDL-C, and TC at different stages. Moreover, maternal probiotics or synbiotics regulated muscle fiber-type, myogenic regulatory, and lipid metabolism-related gene expressions of offspring pigs. Conclusions: In conclusion, maternal probiotics or synbiotics addition, as a nutritional intervention strategy, improved feed intake and meat quality by altering the body's metabolism of offspring pigs and gene expressions related to meat quality.
Predictive Values of the Selected Inflammatory Index in the Progression of Colon Cancer Patients
Authors: Huang Li , Hu Zuojian , Luo Ruixian , Li Hailan , Yang Ziji , Mo Zhuning 
Published: October 2021

Abstract
Background: Ample evidence has revealed that the lymphocyte-to-monocyte ratio (LMR), albumin-to-globulin ratio (AGR) and mean platelet volume (MPV) are cancer-related inflammatory markers. The present study aimed to assess a better diagnostic marker for the progression of colon cancer. Methods: This retrospective study enrolled 251 patients with colon cancer, 171 patients with benign colon diseases, and 187 healthy control subjects from January 2012 to September 2020. The receiver operating characteristic (ROC) curve and area under the curve (AUC) were used to determine the diagnostic values of the selected inflammatory index.Results: The levels of LMR, AGR and MPV were decreased in the colon cancer group compared with the healthy control and benign colon disease groups. The LMR, AGR and MPV were all correlated with tumor size. Moreover, LMR and AGR was associated with lymph node metastasis and clinical stage, AGR was related to distant metastasis. Both the LMR (p = 0.030) and AGR (p = 0.005) were negatively correlated with the concentration of carcinoembryonic antigen (CEA). The AUC value of MPV combined with CEA had a good diagnostic ability for distinguishing controls from colon cancer cases (AUC = 0.950) and patients with benign colon diseases (AUC = 0.886). Meanwhile, the combination of LMR or AGR with CEA could enhance the diagnostic efficacy (AUC; 0.746 for LMR + CEA, 0.737 for AGR + CEA) of detecting colon cancer from benign colon diseases. Conclusions: CEA combined with the selected inflammatory index may be used as better blood-based biomarkers in the progression of colon cancer patients.
Identifying Key Factors Associated with Subscapularis Tendon Tears and Developing a Risk Prediction Model to Assist Diagnosis
Authors: xu Wennan , wang Fei , xue Qingyun 
Published: October 2021

Abstract

Background: Subscapularis (SSC) tendon tears can cause pain and restricted motion of the shoulder, but accurate diagnosis of this lesion on magnetic resonance imaging (MRI) is challenging, especially in small and partial tears. There are no studies that have established a method to reliably assess the risk of subscapularis tendon tears.Methods: Data on 460 patients who received shoulder arthroscopic surgery with preoperative shoulder MRI were collected retrospectively. Of these, patients with SSC tendon tears were defined as the SSC tear group, and patients with intact subscapularis tendon were enrolled in the non-SSC tear group. Logistic regression analysis was used to identify the risk factors of SSC tendon tears which were then incorporated into the nomogram. Results: Among 22 candidate factors, five independent factors including coracohumeral distance CHD (oblique sagittal) (OR, 0.75; 95%CI, [0.67-0.84]), fluid accumulation (Y-face) (OR, 2.29; 95%CI, [1.20-4.38]), long head of biceps tendon (LHB) dislocation/subluxation (OR, 3.62; 95%CI, [1.96-6.68]), number of posterosuperior (PS) rotator cuff tears (OR, 5.36; 95%CI, [3.12-9.22]), and MRI diagnosis (OR, 1.88; 95%CI, [1.06-3.32]) were identified as key predictors associated with subscapularis tendon tears. Incorporating these predictors, the nomogram achieved a good C index with a good agreement on the risk estimation of calibration plots. Higher total points of the nomogram were associated with a greater risk of subscapularis tendon tears. Conclusion: The diagnostic accuracy of conventional 3.0-T MRI for SSC tendon tears was insufficient. Our study revealed critical predictors associated with subscapularis tendon tears. When evaluating the severity of subscapularis tendon injury, more attention should be paid to these tear-related factors. We developed and validated s satisfactory prediction model to improve the diagnostic performance of MRI which was convenient for clinicians to reach a consensus on risk assessment and identify the SSC tendon tears.
Influence of Cusp External Magnetic Field on Deposition Rate of Two-electrode TIG Welding
Authors: Liu Liming , Zhu Yanli , Liu Runtao 
Published: October 2021

Abstract
Experiments have been conducted to investigate the influence of the cusp external magnetic field (EMF) on deposition rate of two-electrode tungsten insert gas (T-TIG) welding. T-TIG arc parameters such as arc shape, arc voltage, arc pressure and arc plasma information were acquired respectively. Results showed compared to TIG, a higher welding current could be allowed for T-TIG, due to its low arc pressure characteristic. Under the effect of the cusp EMF, the arc shape was compressed along x-axis of T-TIG, while elongated along y-axis of T-TIG. Besides, the peak arc pressure of T-TIG was not significantly increased by the cusp EMF. Moreover, with the action of the cusp EMF, the maximum values of the electron temperature (Te) and electron density (Ne) of T-TIG were not significantly increased, but along y-axis, the increments of the two parameters were gradually increased and their distributions were widened. Therefore, more arc energy was allocated on y-axis of T-TIG by the cusp EMF, which could improve the preheating and the melting of the filler wire along y-axis, so the deposition rate of T-TIG could be increased by 17.6% under the effect of the cusp EMF.
WITHDRAWN: Fuzzy reliability analysis based on support vector machine
Authors: Nie xiao bo , Li Haibin , Chen Hongxia , Pang Ruying , Sun Honghua 
Published: October 2021

Abstract
For a structure with implicit performance function structure and less sample data, it is difficult to obtain accurate probability distribution parameters by traditional statistical analysis methods. To address the issue, the probability distribution parameters of samples are often regarded as fuzzy numbers. In this paper, a novel fuzzy reliability analysis method based on support vector machine is proposed. Firstly, the fuzzy variable is converted into an equivalent random variable, and the equivalent mean and equivalent standard deviation are calculated. Secondly, the support vector regression machine with excellent small sample learning ability is used to train the sample data. Subsequently, and the performance function is approximated. Finally, the Monte Carlo method is used to obtain fuzzy reliability. Numerical examples are investigated to demonstrate the effectiveness of the proposed method, which provides a feasible way for fuzzy reliability analysis problems of small sample data.
An Efficient Approach For the Nodal Water Demand Estimation in Large-Scale Water Distribution Systems
Authors: Chu Shipeng , Zhang Tuqiao , Zhou Xinhong , Yu Tingchao , Shao Yu 
Published: October 2021

Abstract
Real-time modeling of the water distribution system (WDS) is a critical step for the control and operation of such systems. The nodal water demand as the most important time-varying parameter must be estimated in real-time. The computational burden of nodal water demand estimation is intensive, leading to inefficiency for the modeling of the large-scale network. The Jacobian matrix computation and Hessian matrix inversion are the processes that dominate the main computation time. To address this problem, an approach to shorten the computational time for the real-time demand estimation in the large-scale network is proposed. The approach can efficiently compute the Jacobian matrix based on solving a system of linear equations, and a Hessian matrix inversion method based on matrix partition and Iterative Woodbury-Matrix-Identity Formula is proposed. The developed approach is applied to a large-scale network, of which the number of nodal water demand is 12523, and the number of measurements ranging from 10 to 2000. Results show that the time consumptions of both Jacobian computation and Hessian matrix inversion are significantly shortened compared with the existing approach.
Far from boring: a new Grenvillian perspective on Mesoproterozoic tectonics
Authors: Gervais Félix , Beaudry Alexandre , Kavanagh-Lepage Charles , Moukhsil Abdelali 
Published: October 2021

Abstract
As determining when plate tectonics began on Earth is a highly debated subject, it is crucial to understand the “boring billion” (1.8 to 0.8 billion years ago), a period of tectonic quiescence inferred from proxies, such as the average chemical composition of the mineral zircon on Earth and the isotopic composition of seawater derived from marine rocks. Yet this period saw the construction of what may have been the biggest mountain belt that ever existed, the remnants of which are found in the Grenville Orogen of eastern North-America. This contribution first exposes a compilation of multidisciplinary geological datasets and new geochemical data from igneous suites emplaced during the Grenvillian Orogeny that are incompatible with the current tectonic paradigm. We then present a completely revised model for Grenvillian tectonics. In contrast with the actual Laurentian-centred paradigm, our model involves the construction of a newly revealed continent by amalgamation of volcanic arcs far away from Laurentia (the craton forming the core of actual North-America) and their collision 60 millions year later than the currently accepted timing. This new model resolves the longstanding contradiction between tectonic proxies and geological record and invalidates the view considering the Mesoproterozoic as a tectonically quiet Era.
Fisetin attenuates Renal Ischemia/Reperfusion Injury by improving mitochondrial quality, reducing apoptosis and oxidative stress
Authors: Prem Priyanka N , Kurian Gino A 
Published: October 2021

Abstract
Purpose
Renal ischemic reperfusion (IR) injury is one of the major source of mortality and morbidity associated with acute kidney injury (AKI). Several flavonoids have shown to be renal protective against many nephrotoxic agents causing AKI. Fisetin, a promising flavonoid, is effective in the management of septic AKI, expected to ameliorate renal IR injury. Present study aim to generate evidence for fisetin mediated renal protection against IR injury
Methodology:
Male wistar rats of 200-250 g subjected to IR protocol by performing bilateral clamping for 45 minutes and reperfusion for 24 hours. Fisetin administrated 30 minutes ( 20 mg/kg b.wt, ip) before the surgery. Renal injury was evaluated by measuring the biomarkers in plasma, examining the ultrastructure of kidney and analyzing the apoptotic changes. Oxidative stress, antioxidant levels and mitochondrial function were analysed in the renal tissue.
Results
Fisetin administration significantly reduced the renal damages associated with IR, by improving estimated glomerular filtration rate (eGFR: IR-0.35 ml/min, F_IR-9.03 ml/min), reducing plasma creatinine level (IR-2.2 mg/dl, F_IR-0.92 mg/dl) and lowering urinary albumin/creatinine ratio (IR- 6.09 F_IR-2.16), caspase activity, decreased DNA fragmentation and reduced tubular injury score (IR- 11 F_IR-6.5). At cellular level, fisetin significantly reduced renal oxidative stress and augmented the antioxidant levels. Fisetin found to preserve mitochondrial electron transport chain activities and improved the ATP producing capacity in the renal tissue upon IR injury.
Conclusion
fisetin pre-treatment attenuate renal IR injury by improving the renal function, reducing the renal injury mediated by apoptosis, reducing free radical release, and augmenting mitochondrial function.
Enterovirus 71 structural viral protein 1 promotes autophagy by inducing an m6A-mediated PMP22 overexpression in mouse Schwann cells
Authors: Liu Guangming , Zhu Danping , Hu Dandan , Li Suyun , Peng Qiuyan , Li Peiqing , Yang Sida 
Published: October 2021

Abstract
Objective

Enterovirus 71 (EV71), one of the enteroviruses responsible for the hand, foot and mouth disease (HFMD), can cause severe neurologic diseases such as brainstem encephalitis and demyelination. The molecular mechanism of demyelination is still not fully understood. This study aims to investigate the mechanism of how the EV71 structural viral protein 1, VP1 can act on host cellular pathways in mouse Schwann cells.
Methods

An EV71 VP1-expressing vector was generated and transfected into mouse Schwann cells (MSCs). Selective mRNA methylation inhibitor (DAA) was employed to identify key members of m6A pathway that are targeted by VP1. To investigate the role of METTL14 and YTHDF1 in PMP22 expression, small interfering RNA against METTL14 and YTHDF1 was employed to knockdown the METTL14 and YTHDF1 expression in MSCs. Real-time PCR and Western blot analysis were performed to determine the expression of PMP22 and m6A modification-associated proteins.
Results

Our results demonstrated VP1 upregulated m6A pathway by targeting METTL14 and YTHDF1. The expression of PMP22 was decreased by inhibiting the expression of METTL14 and YTHDF1.
Conclusions

VP1 upregulates m6A modification, which in turn causes the hypermethylation of PMP22 in MSCs, resulting in a higher level of PMP22. Ultimately, this VP1-induced PMP22 overexpression leads to MSC autophagy.
Identification of ATFs in Human Breast Cancer by Integrated Bioinformatic Analysis
Authors: Geng chenchen , Pu Qian , Tian Shuxu , Geng Wenwen , Wang Haiyan , Zhao Guanghui , Gao Haidong 
Published: October 2021

Abstract
Background: To obtain a thorough comprehension of the profile and prognosis of activating transcription factor (ATF) family members in breast cancer.Method: We searched Oncomine, GEPIA, cBioPortal, Kaplan-Meier plotter, and CancerSEA databases to assess expression level, prognostic value, and functions of ATFs in breast cancer. Results: In breast cancer, we found that the expression levels of genes like ATF1, ATF5, and ATF6, were higher than in normal tissues. While the expression levels of ATF3, ATF4, ATF7 were lower in the former than in the latter. Similarly, the ATFs protein expressions were consistent with this in the Human Protein Atlas database. High expressions of ATF2, ATF4, and ATF6-7 were associated with good relapse-free survival. Increased expressions of ATF4 and ATF7 had high overall survival. Conversely, the mRNA expression of ATF1 was negatively correlated with distant metastasis-free survival. Similarly, high expression of ATF2 had reduced post-progression survival. Conclusions: ATF1 was a target of potential therapeutic interest for breast cancer, and ATF4 and ATF6-7 were potential prognostic factors in evaluating breast cancer.
Effects of Steam Treated Rice Straw-Based Diet on the Physico-Chemical Properties of Goats Longissimus Muscle
Authors: Naeem Muhammad , Ali Sher , Rajput Nasir Ali , Bilal Muhammad , Wang Tian 
Published: October 2021

Abstract
The present study was carried out to investigate the effects of steam treated rice straw-based diets on, physico-chemical properties of longissimus muscle of goat. Goats were assigned to rice straw treated with steam at 15.5 kgf/cm2 for 120 Sec (STRS) and untreated rice (UTRS) straw-based diets. After 60 days all goats were slaughtered and 50 g of muscle longissimus dorsi (LD) was removed from left side of carcass for determination of meat quality and fatty acid profile. The results showed that the steam treated rice straw diet improved (P < 0.05) carcass yield and dressing % of goats as compared to untreated rice straw fed goats. Significantly higher protein and lower moisture was observed for meat of STRS group than UTRS, however the fat, ash contents, meat color and pH were not different between the groups. The TBARS values gradually increased in stored meat. The different diet did not affect the composition of fatty acids. Total saturated (∑SFA) monounsaturated fatty acids (∑MUFA) for UTRS and total polyunsaturated fatty acid (∑PUFA) for STRS group was higher (P > 0.05). It is concluded that the goats fed on treated straw diet (STRS) maintained carcass yield, dressing % and meat composition without negative impact on meat characteristics.
Development of a radiographic scoring system for new bone formation in gout
Authors: Son Chang-Nam , Cai Ken , Stewart Sarah , Ferrier John , Billington Karen , Tsai Yun-Jung Jack , Bardin Thomas , Horne Anne , Stamp Lisa K. , Doyle Anthony , Dalbeth Nicola 
Published: October 2021

Abstract
Background
Features of new bone formation (NBF) are common in tophaceous gout. The aim of this project was to develop a plain radiographic scoring system for NBF in gout.
Methods
Informed by a literature review, scoring systems were tested in 80 individual 1st and 5th metatarsophalangeal joints. Plain radiography scores were compared with computed tomography (CT) measurements of the same joints. The best-performing scoring system was then tested in paired sets of hand and foot radiographs obtained over two years from an additional 25 patients. Inter-reader reproducibility was assessed using intra-class correlation coefficients (ICC). NBF scores were correlated with plain radiographic erosion scores (using the gout-modified Sharp-van der Heijde system).
Results
Following a series of structured reviews of plain radiographs and scoring exercises, a semi-quantitative scoring system for sclerosis and spur was developed. In the individual joint analysis, the inter-observer ICC (95% CI) was 0.84 (0.76-0.89) for sclerosis and 0.81 (0.72-0.87) for spur. Plain radiographic sclerosis and spur scores correlated with CT measurements (R2 = 0.65-0.74, P < 0.001 for all analyses). For the hand and foot radiograph sets, the inter-observer ICC (95% CI) was 0.94 (0.90-0.98) for sclerosis score and 0.76 (0.65-0.84) for spur score. Sclerosis and spur scores correlated highly with plain radiographic erosion scores (R2 = 0.87 and 0.71 respectively), but not with change in erosion scores over two years (R2 = -0.04 – 0.15).
Conclusion
A semi-quantitative plain radiographic scoring method for assessment of NBF in gout is feasible, valid, and reproducible. This method may facilitate consistent measurement of NBF in gout.
VPS35 Protects Against TMEM230-Mutation-Induced Progressive Locomotor Deficits in Drosophila
Authors: Ma Chao , Wang Xiaobo , Smith Wanli W , Liu Zhaohui 
Published: October 2021

Abstract
BackgroundRecently, four Parkinson’s disease (PD)-linked mutations (Y92C, R141L, 184PGext*5 and 184Wext*5) in transmembrane protein 230 (TMEM230) were identified in PD patients, and these mutations have implications in protein trafficking and neurodegeneration. However, there is a lack of in vivo studies on the roles of PD-related variants of TMEM230 in PD pathogenesis.MethodsIn this study, we generated human wild-type (WT) and mutant TMEM230 (Y92C, R141L, 184PGext*5 and 184Wext*5) transgenic Drosophila using isoform Ⅱ cDNA. ResultsWe found that the expression of TMEM230 184PGext*5 in pan-neurons or dopaminergic neurons in Drosophila induced PD-like phenotypes, which included impaired locomotor ability, a shortened lifespan, reduced TH levels, and increased phosphorylated JNK and cleaved caspase-3 levels. Moreover, rotenone, a common pesticide, enhanced TMEM230-184PGext*5-induced PD-like phenotypes. In contrast, the overexpression of wild-type (WT) VPS35 rescued TMEM230-184PGext*5-induced PD-like phenotypes, while the knockdown of VPS35 by RNA interference (RNAi) or the expression of mutant VPS35 D620N worsened PD-like phenotypes. ConclusionThese results indicate that VPS35, as a downstream effector of TMEM230, plays a critical role in TMEM230-linked JNK/caspase-3 signalling pathways and that mutations in TMEM230 and VPS35 disrupt these pathways, resulting in dopaminergic neurodegeneration and PD-like phenotypes. These findings provide novel insight into the molecular mechanisms of mutant TME230- and VPS35-induced abnormalities underlying the pathogenesis of PD.
Signal Transduction in Light-Oxygen-Voltage Receptors Lacking the Active-Site Glutamine
Authors: Dietler Julia , Gelfert Renate , Kaiser Jennifer , Borin Veniamin , Renzl Christian , Pilsl Sebastian , Ranzani Américo Tavares , de Fuentes Andrés García , Gleichmann Tobias , Diensthuber Ralph , Weyand Michael , Mayer Günter , Schapiro Igor , Möglich Andreas 
Published: October 2021

Abstract
In nature as in biotechnology, light-oxygen-voltage (LOV) photoreceptors perceive blue light to elicit spatiotemporally defined cellular responses. Photon absorption drives thioadduct formation between a conserved cysteine and the flavin chromophore. An equally conserved, proximal glutamine processes the resultant flavin protonation into downstream hydrogen-bond rearrangements. Here, we report that this glutamine, long deemed essential, is generally dispensable. In its absence, several LOV receptors invariably retained productive, if often attenuated, signaling responses. Structures of a LOV paradigm at around 1 Å resolution revealed highly similar light-induced conformational changes, irrespective of whether the glutamine is present. Naturally occurring, glutamine-deficient LOV receptors likely serve as bona fide photoreceptors, as we showcase for a diguanylate cyclase. We propose that without the glutamine, water molecules transiently approach the chromophore and thus propagate flavin protonation downstream. Signaling without glutamine appears intrinsic to LOV receptors, which pertains to biotechnological applications and suggests evolutionary descendance from redox-active flavoproteins.