bluuebunny/crossref_metadata_2025_split huggingface dataset:
A human multi-lineage hepatic organoid model for liver fibrosis
Authors: Guan Yuan , Enejder Annika , Wang Meiyue , Fang Zhuoqing , Cui Lu , Chen Shih-Yu , Wang Jingxiao , Tan Yalun , Wu Manhong , Chen Xinyu , Johansson Patrik K. , Osman Issra , Kunimoto Koshi , Russo Pierre , Heilshorn Sarah C. , Peltz Gary 
Published: October 2021

AbstractTo investigate the pathogenesis of a congenital form of hepatic fibrosis, human hepatic organoids were engineered to express the most common causative mutation for Autosomal Recessive Polycystic Kidney Disease (ARPKD). Here we show that these hepatic organoids develop the key features of ARPKD liver pathology (abnormal bile ducts and fibrosis) in only 21 days. The ARPKD mutation increases collagen abundance and thick collagen fiber production in hepatic organoids, which mirrors ARPKD liver tissue pathology. Transcriptomic and other analyses indicate that the ARPKD mutation generates cholangiocytes with increased TGFβ pathway activation, which are actively involved stimulating myofibroblasts to form collagen fibers. There is also an expansion of collagen-producing myofibroblasts with markedly increased PDGFRB protein expression and an activated STAT3 signaling pathway. Moreover, the transcriptome of ARPKD organoid myofibroblasts resemble those present in commonly occurring forms of liver fibrosis. PDGFRB pathway involvement was confirmed by the anti-fibrotic effect observed when ARPKD organoids were treated with PDGFRB inhibitors. Besides providing insight into the pathogenesis of congenital (and possibly acquired) forms of liver fibrosis, ARPKD organoids could also be used to test the anti-fibrotic efficacy of potential anti-fibrotic therapies.
Targeting miR-126 in inv(16) acute myeloid leukemia inhibits leukemia development and leukemia stem cell maintenance
Authors: Zhang Lianjun , Nguyen Le Xuan Truong , Chen Ying-Chieh , Wu Dijiong , Cook Guerry J. , Hoang Dinh Hoa , Brewer Casey J. , He Xin , Dong Haojie , Li Shu , Li Man , Zhao Dandan , Qi Jing , Hua Wei-Kai , Cai Qi , Carnahan Emily , Chen Wei , Wu Xiwei , Swiderski Piotr , Rockne Russell C. , Kortylewski Marcin , Li Ling , Zhang Bin , Marcucci Guido , Kuo Ya-Huei 
Published: October 2021

AbstractAcute myeloid leukemia (AML) harboring inv(16)(p13q22) expresses high levels of miR-126. Here we show that theCBFB-MYH11 (CM)fusion gene upregulates miR-126 expression through aberrant miR-126 transcription and perturbed miR-126 biogenesis via the HDAC8/RAN-XPO5-RCC1 axis. Aberrant miR-126 upregulation promotes survival of leukemia-initiating progenitors and is critical for initiating and maintaining CM-driven AML. We show that miR-126 enhances MYC activity through the SPRED1/PLK2-ERK-MYC axis. Notably, genetic deletion of miR-126 significantly reduces AML rate and extends survival in CM knock-in mice. Therapeutic depletion of miR-126 with an anti-miR-126 (miRisten) inhibits AML cell survival, reduces leukemia burden and leukemia stem cell (LSC) activity in inv(16) AML murine and xenograft models. The combination of miRisten with chemotherapy further enhances the anti-leukemia and anti-LSC activity. Overall, this study provides molecular insights for the mechanism and impact of miR-126 dysregulation in leukemogenesis and highlights the potential of miR-126 depletion as a therapeutic approach for inv(16) AML.
Optimization of training and measurement protocol for eNose analysis of urine headspace aimed at prostate cancer diagnosis
Authors: Capelli Laura , Bax Carmen , Grizzi Fabio , Taverna Gianluigi 
Published: October 2021

AbstractMore than one million new cases of prostate cancer (PCa) were reported worldwide in 2020, and a significant increase of PCa incidence up to 2040 is estimated. Despite potential treatability in early stages, PCa diagnosis is challenging because of late symptoms’ onset and limits of current screening procedures. It has been now accepted that cell transformation leads to release of volatile organic compounds in biologic fluids, including urine. Thus, several studies proposed the possibility to develop new diagnostic tools based on urine analysis. Among these, electronic noses (eNoses) represent one of the most promising devices, because of their potential to provide a non-invasive diagnosis. Here we describe the approach aimed at defining the experimental protocol for eNose application for PCa diagnosis. Our research investigates effects of sample preparation and analysis on eNose responses and repeatability. The dependence of eNose diagnostic performance on urine portion analysed, techniques involved for extracting urine volatiles and conditioning temperature were analysed. 192 subjects (132 PCa patients and 60 controls) were involved. The developed experimental protocol has resulted in accuracy, sensitivity and specificity of 83% (CI95% 77–89), 82% (CI95% 73–88) and 87% (CI95% 75–94), respectively. Our findings define eNoses as valuable diagnostic tool allowing rapid and non-invasive PCa diagnosis.
Mapping the NFT revolution: market trends, trade networks, and visual features
Authors: Nadini Matthieu , Alessandretti Laura , Di Giacinto Flavio , Martino Mauro , Aiello Luca Maria , Baronchelli Andrea 
Published: October 2021

AbstractNon Fungible Tokens (NFTs) are digital assets that represent objects like art, collectible, and in-game items. They are traded online, often with cryptocurrency, and are generally encoded within smart contracts on a blockchain. Public attention towards NFTs has exploded in 2021, when their market has experienced record sales, but little is known about the overall structure and evolution of its market. Here, we analyse data concerning 6.1 million trades of 4.7 million NFTs between June 23, 2017 and April 27, 2021, obtained primarily from Ethereum and WAX blockchains. First, we characterize statistical properties of the market. Second, we build the network of interactions, show that traders typically specialize on NFTs associated with similar objects and form tight clusters with other traders that exchange the same kind of objects. Third, we cluster objects associated to NFTs according to their visual features and show that collections contain visually homogeneous objects. Finally, we investigate the predictability of NFT sales using simple machine learning algorithms and find that sale history and, secondarily, visual features are good predictors for price. We anticipate that these findings will stimulate further research on NFT production, adoption, and trading in different contexts.
Biosorption of cationic Hg2+ and Remazol brilliant blue anionic dye from binary solution using Gelidium corneum biomass
Authors: El-Ahmady Ali El-Naggar Noura , Hamouda Ragaa A. , El-Khateeb Ayman Y. , Rabei Nashwa H. 
Published: October 2021

AbstractRemazol brilliant blue (RBB) is an anthraquinone anionic dye that has several commercial uses, especially in the textile industries and is well-known for its detrimental impacts on marine life and the surrounding ecosystem. Mercury (Hg2+) is also one of the most severe hazardous environmental contaminants due to its bioaccumulation through the food chain and high toxicity to the human embryo and fetus. The biosorption potential of Gelidium corneum biomass for bioremoval of Hg2+ and RBB dye simultaneously from binary mixture was assessed. The effects of initial pH, contact time, Hg2+, RBB, and biomass concentrations on the biosorption process were investigated in 50 batch experiments using a Face-centered central composite design. The maximum removal percentage of Hg2+ (98.25%) was achieved in the run no. 14, under optimum experimental conditions: 200 mg/L Hg2+, 75 mg/L RBB, pH 5. At 30 °C, 4 g/L algal biomass was used, with a contact time of 180 min. Whereas, the maximum removal percentage of RBB (89.18%) was obtained in the run no. 49 using 200 mg/L Hg2+, 100 mg/L RBB, pH 5, 4 g/L algal biomass and 180 min of contact time. FTIR analysis of Gelidium corneum biomass surface demonstrated the presence of many functional groups that are important binding sites responsible for Hg2+ and RBB biosorption. SEM analysis showed apparent morphological alterations including surface shrinkage and the appearance of new shiny adsorbate ion particles on the Gelidium corneum biomass surface after the biosorption process. The EDX study reveals an additional optical absorption peak for Hg2+, confirming the role of Gelidium corneum biomass in Hg2+ biosorption. In conclusion, Gelidium corneum biomass has been shown to be an eco-friendly, sustainable, promising, cost-effective and biodegradable biosorbent to simultaneously biosorb Hg2+ and RBB dye from aquatic ecosystems.
Whole genome sequencing and protein structure analyses of target genes for the detection of Salmonella
Authors: Hu Lijun , Cao Guojie , Brown Eric W. , Allard Marc W. , Ma Li M. , Zhang Guodong 
Published: October 2021

AbstractRapid and sensitive detection of Salmonella is a critical step in routine food quality control, outbreak investigation, and food recalls. Although various genes have been the targets in the design of rapid molecular detection methods for Salmonella, there is limited information on the diversity of these target genes at the level of DNA sequence and the encoded protein structures. In this study, we investigated the diversity of ten target genes (invA, fimA, phoP, spvC, and agfA; ttrRSBCA operon including 5 genes) commonly used in the detection and identification of Salmonella. To this end, we performed whole genome sequencing of 143 isolates of Salmonella serotypes (Enteritidis, Typhimurium, and Heidelberg) obtained from poultry (eggs and chicken). Phylogenetic analysis showed that Salmonella ser. Typhimurium was more diverse than either Enteritidis or Heidelberg. Forty-five non-synonymous mutations were identified in the target genes from the 143 isolates, with the two most common mutations as T ↔ C (15 times) and A ↔ G (13 times). The gene spvC was primarily present in Salmonella ser. Enteritidis isolates and absent from Heidelberg isolates, whereas ttrR was more conserved (0 non-synonymous mutations) than ttrS, ttrB, ttrC, and ttrA (7, 2, 2, and 7 non-synonymous mutations, respectively). Notably, we found one non-synonymous mutation (fimA-Mut.6) across all Salmonella ser. Enteritidis and Salmonella ser. Heidelberg, C → T (496 nt postion), resulting in the change at AA 166 position, Glutamine (Q) → Stop condon (TAG), suggesting that the fimA gene has questionable sites as a target for detection. Using Phyre2 and SWISS-MODEL software, we predicted the structures of the proteins encoded by some of the target genes, illustrating the positions of these non-synonymous mutations that mainly located on the α-helix and β-sheet which are key elements for maintaining the conformation of proteins. These results will facilitate the development of sensitive molecular detection methods for Salmonella.
Wnt6 plays a complex role in maintaining human limbal stem/progenitor cells
Authors: Bonnet Clémence , Oh Denise , Mei Hua , Robertson Sarah , Chang Derek , Bourges Jean-Louis , Behar-Cohen Francine , Zheng Jie J. , Deng Sophie X. 
Published: October 2021

AbstractThe corneal epithelium is consistently regenerated by limbal stem/progenitor cells (LSCs), a very small population of adult stem cells residing in the limbus. Several Wnt ligands, including Wnt6, are preferentially expressed in the limbus. To investigate the role of Wnt6 in regulating proliferation and maintenance of human LSCs in an in vitro LSC expansion setting, we generated NIH-3T3 feeder cells to overexpress different levels of Wnt6. Characterization of LSCs cultured on Wnt6 expressing 3T3 cells showed that high level of Wnt6 increased proliferation of LSCs. Medium and high levels of Wnt6 also increased the percentage of small cells (diameter ≤ 12 µm), a feature of the stem cell population. Additionally, the percentage of cells expressing the differentiation marker K12 was significantly reduced in the presence of medium and high Wnt6 levels. Although Wnt6 is mostly known as a canonical Wnt ligand, our data showed that canonical and non-canonical Wnt signaling pathways were activated in the Wnt6-supplemented LSC cultures, a finding suggesting that interrelationships between both pathways are required for LSC regulation.
Investigation of the out-of-plane load-bearing capacity of T1100/5405 composite T-stiffened panels
Authors: Huang Heyuan , Zhang Xuanjia , Dong Zhicheng , Wang Dong 
Published: October 2021

AbstractWith the continuous improvement of the mechanical properties of composite materials, the adhesive interface performance of composite T-stiffened panels has become a critical factor in determining the overall structural strength. However, little work has been reported on the mechanical properties of adhesive interfaces in composite T-stiffened panels under lateral bending and shear loading. Especially, there is no clear explanation on the damage evolution law of structural properties for the interface with defects, which greatly influenced the use of T-stiffened composite structures. In this paper, the mechanical properties of T1100/5405 composite T-stiffened laminates under lateral bending and shear loading are experimentally and numerically investigated. The load-bearing capacities for the panels with intact and defected adhesive interfaces are compared, the damage evolution law of typical T-stiffened structures is further explored. Based on the continuum damage model (CDM) and the cohesive zone model (CZM), the constitutive models of the adhesive layer and the composite material are established respectively. Good agreements between experimental and numerical profiles illustrate that damages mainly occur on the loading side and the corner of the L-type ribs under lateral bending conditions, while damages extend from both sides of the interface layer to the center under shear loading. When a prefabricated defect exists, damages extend from the defect location along the loading direction. At the same time, the analysis shows that the lay-up of the surface layer, the chamfer radius, and the width of T-type ribs have a great influence on the structural load-bearing capacity, but less on the damage evolution form.
Field evidence for microplastic interactions in marine benthic invertebrates
Authors: Vecchi Stefania , Bianchi Jessica , Scalici Massimiliano , Fabroni Fabrizio , Tomassetti Paolo 
Published: October 2021

AbstractMicroplastics represent an important issue of concern for marine ecosystems worldwide, and closed seas, such as the Mediterranean, are among the most affected by this increasing threat. These pollutants accumulate in large quantities in benthic environments causing detrimental effects on diverse biocenoses. The main focus of this study is on the ‘polychaetes-microplastics’ interactions, particularly on two species of benthic polychaetes with different ecology and feeding strategies: the sessile and filter feederSabella spallanzanii(Gmelin, 1791) and the vagile carnivorousHermodice carunculata(Pallas, 1766). Since not standardized protocols are proposed in literature to date, we compared efficiencies of diverse common procedures suitable for digesting organic matter of polychaetes. After the definition of an efficient digestion protocol for microplastics extraction for both polychaetes, our results showed high microplastics ingestion in both species. Microplastics were found in 42% of individualsof S. spallanzanii, with a mean of 1 (± 1.62) microplastics per individual, in almost all individuals ofH. carunculata(93%), with a mean of 3.35 (± 2.60). These significant differences emerged betweenS. spallanzaniiandH. carunculata, is probably due to the diverse feeding strategies. The susceptibility to this pollutant makes these species good bioindicators of the impact of microplastics on biota.
Clemastine improves electrophysiologic and histomorphometric changes through promoting myelin repair in a murine model of compression neuropathy
Authors: Lee Jung Il , Park Jong Woong , Lee Kyung Jun , Lee Duk Hee 
Published: October 2021

AbstractCompression neuropathies are common and debilitating conditions that result in variable functional recovery after surgical decompression. Recent drug repurposing studies have verified that clemastine promotes functional recovery through enhancement of myelin repair in demyelinating disease. We investigated the utility of clemastine as a treatment for compression neuropathy using a validated murine model of compression neuropathy encircling the compression tube around the sciatic nerve. Mice received PBS or clemastine solution for 6 weeks of compression phase. Mice taken surgical decompression received PBS or clemastine solution for 2 weeks of decompression phase. Electrodiagnostic, histomorphometric, and Western immunoblotting analyses were performed to verify the effects of clemastine. During the compression phase, mice treated with clemastine had significantly decreased latency and increased amplitude compared to untreated mice that received PBS. Histomorphometric analyses revealed that mice treated with clemastine had significantly higher proportions of myelinated axons, thicker myelin, and a lower G-ratio. The expression levels of myelin proteins, including myelin protein zero and myelin associated glycoprotein, were higher in mice treated with clemastine. However, the electrophysiologic and histomorphometric improvements were observed regardless of clemastine treatment in mice taken surgical decompression. Mice treated with clemastine during compression of the sciatic nerve demonstrated that clemastine treatment attenuated electrophysiologic and histomorphometric changes caused by compression through promoting myelin repair.
Cumulative DNA damage by repeated low-dose cisplatin injection promotes the transition of acute to chronic kidney injury in mice
Authors: Yamashita Noriyuki , Nakai Kunihiro , Nakata Tomohiro , Nakamura Itaru , Kirita Yuhei , Matoba Satoaki , Humphreys Benjamin D. , Tamagaki Keiichi , Kusaba Tetsuro 
Published: October 2021

AbstractCisplatin is a commonly used anticancer drug, but nephrotoxicity is a dose-limiting adverse effect. Recent experimental and clinical observations have demonstrated that multiple injections of cisplatin induce the transition to chronic kidney disease; however, the underlying mechanisms remain unclear. We found that multiple injections of higher doses of cisplatin in a shorter interval affected the severity of kidney injury, causing kidney fibrosis to develop at a later time point. An additional injection of cisplatin during the recovery period after a prior injury, when proximal tubule epithelia are actively proliferating, induced substantial tubular injury by inducing more severe DNA damage than that induced by a single injection. Lineage tracing analysis of proximal tubular epithelia demonstrated that the tubular epithelia that underwent multiple rounds of cell division after multiple injections of cisplatin existed at the chronic phase, and these populations often expressed vcam1 + , suggesting the induction of proinflammatory failed-repair tubular epithelia. Our study revealed that as cisplatin exerts cytotoxic effects on actively proliferating cells, additional cisplatin injections before the completion of tubular repair exacerbates kidney injury through cumulative DNA damage. Appropriate both the setting of dosage and dosing intervals, with careful monitoring, are essential to prevent nephrotoxicity of repeated cisplatin treatment in cancer patients.
Paediatric Trauma Score as a non-imaging tool for predicting intracranial haemorrhage in patients with traumatic brain injury
Authors: Kim Heoung Jin , Eun Sohyun , Yoon Seo Hee , Kim Moon Kyu , Chung Hyun Soo , Koo Chungmo 
Published: October 2021

AbstractTo identify a useful non-imaging tool to screen paediatric patients with traumatic brain injury for intracranial haemorrhage (ICH). We retrospectively analysed patients aged < 15 years who visited the emergency department with head trauma between January 2015 and September 2020. We divided patients into two groups (ICH and non-ICH) and compared their demographic and clinical factors. Among 85 patients, 21 and 64 were in the ICH and non-ICH groups, respectively. Age (p = 0.002), Pediatric trauma score (PTS; p < 0.001), seizure (p = 0.042), and fracture (p < 0.001) differed significantly between the two groups. Factors differing significantly between the groups were as follows: age (odds ratio, 0.84, p = 0.004), seizure (4.83, p = 0.013), PTS (0.15, p < 0.001), and fracture (69.3, p < 0.001). Factors with meaningful cut-off values were age (cut-off [sensitivity, specificity], 6.5 [0.688, 0.714], p = 0.003) and PTS [10.5 (0.906, 0.81), p < 0.001]. Based on the previously known value for critical injury (≤ 8 points) and the cut-off value of the PTS identified in this study (≤ 10 points), we divided patients into low-risk, medium-risk, and high-risk groups; their probabilities of ICH (95% confidence intervals) were 0.16–12.74%, 35.86–89.14%, and 100%, respectively. PTS was the only factor that differed significantly between mild and severe ICH cases (p = 0.012). PTS is a useful screening tool with a high predictability for ICH and can help reduce radiation exposure when used to screen patient groups before performing imaging studies.
Non fourier heat transfer enhancement in power law fluid with mono and hybrid nanoparticles
Authors: Sadiq M. Adil 
Published: October 2021

AbstractSeveral polymers like ethylene glycol exhibit non-Newtonian rheological behavior. Ethylene glycol is a world-widely used engine coolant and therefore, investigation of thermal enhancement by dispersing mono and hybrid nanoparticles in ethylene glycol is worthful. Since ethylene glycol has shear rate-dependent viscosity and it obeys the power-law rheological model. Therefore, based on these facts, the power-law rheological model with thermophysical properties is augmented with basic law of heat transfer in fluid for the modeling of the considered physical situation. $$Mo{S}_{2}$$

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are taken as mono-nanoparticles where $$Mo{S}_{2}$$

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and $$Si{O}_{2}$$

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are taken as hybrid nanoparticles. Comparative study for the enhancement of thermal performance of MoS2 ethylene glycol and $$Mo{S}_{2}$$

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−$$Si{O}_{2}$$

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– ethylene glycol is done. For energy conservation, non-Fourier’s law of Cattaneo–Christov is used. The power-law fluid becomes more heat generative due to the dispersion of $$Mo{S}_{2}$$

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and $$Si{O}_{2}$$

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. However, $$Mo{S}_{2}$$

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−power-law fluid is less heat generative relative to $$Mo{S}_{2}$$

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− $$Si{O}_{2}$$

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-nanofluid. Thermal relaxation time is found proportional to the ability of the fluid to restore its thermal equilibrium.