bluuebunny/crossref_metadata_2025_split huggingface dataset:
Temporal Dynamics of the Tomato Rhizosphere Microbiome in Response to Synthetic Communities of Plant Growth-Promoting Rhizobacteria
Authors: Nicotra Daniele , Mosca Alexandros , Dimaria Giulio , Tessitori Matilde , Vetukuri Ramesh Raju , Catara Vittoria 
Published: December 2024

AbstractIn sustainable agriculture, the application of microorganisms to soil is a widely adopted strategy aimed at enhancing soil microbiome functionality, restoring fertility, and recovering biodiversity diminished by intensive farming. While introducing individual beneficial microbes often encounters issues with establishment and persistence, multispecies microbial consortia offer a more robust alternative, providing complementary functions that enhance both their resilience and effectiveness. To test this approach, we designed three synthetic bacterial communities (SynComs), each composed of varying combinations of ten bacterial endophytes previously shown to promote plant growth and exert biocontrol effects. The SynComs were designed with ascending levels of richness (4, 6, and 10 members) and diversity (ranging from 3 to 6 bacterial genera). In growth chamber trials with tomatoes, the SynComs not only promoted plant growth but also induced significant shifts in the rhizosphere bacterial communities, primarily affecting less abundant taxa. The SynComs MIX2 and MIX3, which includedPseudomonasspecies, exhibited the greatest impact on both plant growth enhancement and shifts within the resident microbial community. Monitoring of the introduced strains over time demonstrated that most bioinoculants successfully established although at very low concentration in the rhizosphere.
Experimental Murine Periodontitis Increases Salivary Gland IgA‐Producing B Cells Following Oral Dysbiosis
Authors: Nara Mai , Kurosawa Mie , Itsumi Momoe , Morisaki Hirobumi , Fukamachi Haruka , Okahashi Nobuo , Suzuki Noriyuki , Kuwata Hirotaka 
Published: December 2024

AbstractThe oral microbiome is closely involved in the maintenance of host health and the development of systemic diseases. The salivary glands play an essential role in homeostasis in the oral cavity. Here, we investigated the effects of periodontal inflammation on salivary gland function and the oral microbiome. In experimental periodontitis model mice, an increase in IgA⁺ cells in the salivary glands were observed 1 week after treatment. Alteration of the oral microbiome was also induced in this model. Gene expression analysis of the salivary glands showed changes in the expression of genes related to B‐cell maturation and plasma cell differentiation and an increase in the expression of genes related to macrophage activation upon experimental periodontitis induction. Furthermore, the relationship between disruption of oral microflora and salivary gland function was examined using a cohousing model in which experimental periodontitis model mice and untreated mice were reared in the same cage. We found that cohoused normal mice underwent alteration of the oral microbiome, with increases in IgA⁺ cells and macrophages in the salivary glands. In summary, our results suggest that, in the oral cavity, there is a close link between oral bacterial flora and immune cells in the salivary glands. Our results also show that localized inflammation disrupts the homeostasis in the oral cavity, inducing pathological conditions such as dysbiosis. Our study suggests the importance of the interaction among local oral inflammation, salivary gland function, and oral microflora, and provides new insights into the mechanisms by which oral health is maintained.
Medical test and employee's autonomy. Confidentiality of data and non‐discrimination
Authors: Muñiz Javier Fernández‐Costales 
Published: December 2024

AbstractMonitoring health is one of the basic principles of Occupational Health and Safety. The main objective of this monitoring will be the detection of possible damage to health arising from work. They try to discover the effects that the inherent risks with the work may cause the worker, which will show, given the case, through an alteration of health or the state of organic and functional state, both physically and mentally. Regarding the monitoring of health, there are many and varied issues to be raised concerning the right to personal privacy. Thus, notably, whether there was effective consensus, and whether, in particular, the object and the purpose of the medical examination meant a disregard of the statutory scheme, an excess or even a failure to comply with the terms of the Spanish Constitution. The limits imposed by the law makers to the productive unit will play a key role in identifying the content of the company obligation to monitor health, being in many cases guarantees of constitutional rights. Therefore, the methods of health monitoring and control of workers will always be carried out respecting the privacy and dignity of the worker as well as the confidentiality of all information related to their health.
Relationships between trunk tissue distribution, metabolic risk factors and physical performance in young people—A pilot study
Authors: Ishiguro‐Tanaka Noriko , Kitagawa Funa , Akima Hiroshi 
Published: December 2024

AbstractThe present study examined the relationships between trunk tissue distribution, metabolic risk factors, and physical performance in young Japanese individuals using cross‐sectional and longitudinal analyses. Thirty‐six healthy Japanese men (n = 20, body mass index [BMI]: 20.8 ± 2.0 kg/m2) and women (n = 16, BMI: 19.6 ± 2.0 kg/m2) aged 20–26 years old visited our laboratory twice with an interval of 1 year. The thicknesses of skeletal muscle (MT), subcutaneous adipose tissue (SCAT), and the intra‐abdominal cavity (IAT) were assessed by ultrasound imaging and adjusted by body mass1/3 (BM1/3). Blood properties related to hepatic function or metabolic syndrome, brachial‐ankle pulse wave velocity, hand grip strength, two‐step‐length/height scores, and sit‐and‐reach test scores were also measured. As a result of the cross‐sectional analysis, significant relationships were observed between SCAT/BM1/3 and indices of glucose metabolism (HOMA‐IR and QUICKI) in men (r = 0.513 and ‐0.583), and between IAT/BM1/3 and fasting blood glucose in women (r = 0.524). Longitudinal analyses of women showed that changes (%) in IAT and MT/IAT correlated with % changes in the indices of hepatic function (AST) and glucose metabolism (HOMA‐IR and HOMA‐β) (r = −0.673 to 0.686). Significant correlations were also observed between MT/IAT and walking ability (two‐step‐length/height) in cross‐sectional and longitudinal analyses of men (r = 0.463 and 0.525). In conclusion, the trunk tissue distribution could be used to detect the early symptoms of metabolic risks and declines in physical performance in young men and women.
Sudden unexpected infant death, sudden unexplained death in childhood, and sudden unexpected death in epilepsy
Authors: Sharma Suvasini , Whitney Robyn , Chowdhury Sayoni Roy , Ramachandrannair Rajesh 
Published: December 2024

AbstractSudden deaths in infants and children represent a profound and tragic event that continues to challenge researchers despite extensive investigation over several decades. The predominant phenotype, sudden infant death syndrome (SIDS), has evolved into the broader category of sudden unexpected infant death (SUID). In older children, a less understood phenomenon known as sudden unexplained death in childhood (SUDC) has garnered attention. Additionally, sudden unexpected death in epilepsy (SUDEP) constitutes a rare but recognized complication of epilepsy. Recent investigations indicate overlapping clinical, neuropathological, and genetic characteristics among SUID, SUDC, and SUDEP. Common features include death occurring during sleep, discovery in the prone position, hippocampal abnormalities, and genetic variations associated with epilepsy or cardiac arrhythmias. Notably, video recordings in certain examples of SUDC have captured ‘convulsive’ episodes preceding death in children without prior seizure history, suggesting that seizures may contribute more significantly to sudden paediatric deaths than previously presumed. This review explores these shared elements, underscoring their importance in formulating possible preventative measures against these devastating conditions.
Mixed financing strategies for a supply chain with a financially distressed seller: combination of equity financing and debt financing
Authors: Yan Qiang , Wang Chengfu 
Published: December 2024

AbstractThis work discusses a supply chain composed of one third‐party logistics (3PL) firm and one capital‐constrained seller. To cover the seller's ordering and logistics costs, two distinct mixed financing schemes are considered to address the funding needs: (i) equity financing and bank credit financing (EBF) and (ii) equity financing and 3PL firm financing (ELF). We examine how the equity financing ratio influences equilibrium outcomes and derive the financing preference for each member. The results reveal that a large equity financing ratio prompts the seller to buy more. Interestingly, the seller's profit under EBF either monotonically decreases or exhibits a concave relationship with respect to the equity financing ratio. By contrast, the seller's profit under ELF is consistently concave with respect to the equity financing ratio. Additionally, a Pareto zone of the 3PL firm's interest rate exists in which a win–win situation can be achieved. The seller tends to opt for ELF when the sum of the unit transportation fee and ordering cost is high but the equity financing ratio is moderate. Comparatively, the 3PL firm is reluctant to offer financing when both the sum of the unit transportation fee and ordering cost, and the sum of the unit transportation cost and ordering cost are high, while the equity financing ratio is low. Finally, we investigate an endogenous equity financing ratio and further discuss the effect of the seller's initial working capital.
Safety and efficacy of moderate‐intensity statin with ezetimibe in elderly patients with atherosclerotic cardiovascular disease
Authors: Cha Jung‐Joon , Kim Ju Hyeon , Hong Soon Jun , Lim Subin , Joo Hyung Joon , Park Jae Hyoung , Yu Cheol Woong , Lee Pil Hyung , Lee Seung Whan , Lee Cheol Whan , Moon Jae Youn , Lee Jong‐Young , Kim Jung‐Sun , Park Jae Suk , Lim Do‐Sun 
Published: December 2024

AbstractBackgroundHigh‐intensity statin therapy significantly reduces mortality and cardiovascular events in patients with atherosclerotic cardiovascular disease (ASCVD). However, moderate‐intensity statins are often preferred for elderly patients due to their higher risk of intolerance to high‐intensity statins.ObjectiveTo compare the incidence of statin‐associated muscle symptoms (SAMS) and the effect on low‐density lipoprotein cholesterol (LDL‐C) levels between elderly ASCVD patients receiving high‐intensity statin monotherapy and those receiving moderate‐intensity statin with ezetimibe in a combination therapy.MethodIn a prospective, multicenter, open‐label trial conducted in South Korea, 561 patients aged 70 years or above with ASCVD were randomly assigned to receive either moderate‐intensity statin with ezetimibe combination therapy (rosuvastatin 5 mg with ezetimibe 10 mg) or high‐intensity statin monotherapy (rosuvastatin 20 mg) over 6 months. The primary endpoint was the incidence of SAMS, and the key secondary endpoint was the achievement of target LDL‐C levels (<70 mg/dL) within 6 months.ResultsThe primary endpoint showed a lower incidence of SAMS in the combination therapy group (0.7%) compared to the high‐intensity statin monotherapy group (5.7%, p = 0.005). Both groups achieved similar LDL‐C levels, with 75.4% in the combination therapy group and 68.7% in the monotherapy group reaching target levels.ConclusionModerate‐intensity statin with ezetimibe combination therapy offers a lower risk of SAMS and similar LDL‐C reduction in elderly patients with ASCVD, compared to high‐intensity statin monotherapy.
Comparative efficacy of liberal and restrictive blood transfusion strategies in anemic patients with acute myocardial infarction: A systematic review and meta‐analysis of randomized controlled trials
Authors: Kow Chia Siang , Ramachandram Dinesh Sangarran , Hasan Syed Shahzad , Thiruchelvam Kaeshaelya 
Published: December 2024

AbstractIntroductionAcute myocardial infarction (AMI) poses a significant global health burden, warranting meticulous management strategies, particularly in patients with concurrent anemia. Blood transfusion strategies play a pivotal role in optimizing oxygen delivery while minimizing transfusion‐related risks. Two contrasting approaches, liberal and restrictive transfusion strategies, have emerged, yet their comparative effectiveness remains uncertain due to conflicting evidence.MethodsWe conducted a systematic review and meta‐analysis focusing on randomized controlled trials (RCTs) comparing liberal versus restrictive transfusion strategies in AMI patients with anemia (hemoglobin levels <10 g/dL). Comprehensive searches were performed across electronic databases up to March 31, 2024. Data extraction, risk of bias assessment using Cochrane's RoB 2 tool, and meta‐analysis were conducted following methodology.ResultsAmong 327 initially identified studies, four high‐quality RCTs met the inclusion criteria. These trials encompassed varied sample sizes and patient demographics. Meta‐analysis revealed no significant difference in 30‐day all‐cause mortality nor recurrent MI between liberal and restrictive transfusion strategies. However, combining these outcomes into a composite measure demonstrated a significant reduction favoring liberal transfusion (pooled odds ratio = 0.82; 95% confidence interval = 0.69–0.99).ConclusionWhile liberal transfusion strategies show promise in reducing adverse outcomes, clinical decision‐making should be guided by individual patient factors and preferences. Personalized care remains paramount in determining the most appropriate transfusion approach for AMI patients with anemia. Further research is warranted to elucidate the optimal transfusion strategy in this population.
Electrical stimulation of injured nerves promotes recovery in animals and humans
Authors: Gordon Tessa 
Published: December 2024

AbstractThe frequent poor functional outcomes after delayed surgical repair of injured human peripheral nerves results in progressive downregulation of growth‐associated genes in parallel with reduced neuronal regenerative capacity under each of the experimental conditions of chronic axotomy of neurones that remain without target contact, chronic distal nerve stump denervation, and chronic muscle denervation. Brief (1 h) low‐frequency (20 Hz) electrical stimulation (ES) accelerates the outgrowth of regenerating axons across the surgical site of microsurgical repair of a transected nerve. Exercise programmes also promote nerve regeneration with the combination of ES and exercise being the most effective. An ES conditioning lesion of intact nerve (CES) accelerates both axonal outgrowth and regeneration rate after the surgical repair of a more distal injury to the nerve, in contrast to ES of a repaired injury nerve that accelerates only the axon outgrowth. A CES accelerates both axonal outgrowth and regeneration rate after the surgical repair of a more distal injury to the nerve, in contrast to ES of a repaired injury nerve that accelerates only the axon outgrowth. The loss of contractility of permanently denervated muscles in cauda equinae‐injured patients with accompanying severe loss of muscle mass, disarray of thick and thin contractile filaments, and disorganization of the sarcoplasmic reticulum that controls calcium delivery to the filaments, is alleviated by a 2‐year programme of daily ES of the quadriceps muscle. These findings hold promise for recovery and rehabilitation in patients who suffer injury to the neuromuscular system.
imageKey points
Poor functional outcomes after delayed surgical repair of injured human peripheral nerves are replicated by chronic neuronal axotomy, Schwann cell denervation in a nerve autograft, and muscle denervation.
Exponential decline in expression of growth‐associated genes accompanies the same decline in regenerative capacity.
Brief (1 h) low‐frequency (20 Hz) electrical stimulation (ES) that generates action potential conduction to the neuronal soma accelerates the outgrowth of regenerating axons across the surgical repair site of the transected nerve, even after delayed surgery.
The same ES regimen accelerates muscle reinnervation in patients with chronic nerve injury who undergo carpal tunnel syndrome release surgery.
A 2‐year programme of daily ES of permanently denervated quadriceps muscles in cauda equinae‐injured patients reinstated their contractility and organization.
CSF1‐R inhibition attenuates posttraumatic osteoarthritis and quadriceps atrophy following ligament injury
Authors: Keeble Alexander R. , Thomas Nicholas T. , Balawender Peyton J. , Brightwell Camille R. , Gonzalez‐Velez Sara , O'Daniel Madeline G. , Conley Caitlin E. , Stone Austin V. , Johnson Darren L. , Noehren Brian , Jacobs Cale A. , Fry Christopher S. , Owen Allison M. 
Published: December 2024

AbstractKnee osteoarthritis contributes substantially to worldwide disability. Post‐traumatic osteoarthritis (PTOA) develops secondary to joint injury, such as ligament rupture, and there is increasing evidence suggesting a key role for inflammation in the aetiology of PTOA and associated functional deficits. Colony stimulating factor 1 receptor (CSF1‐R) has been implicated in the pathogenesis of musculoskeletal degeneration following anterior cruciate ligament (ACL) injury. We sought to assess the efficacy of CSF1‐R inhibition to mitigate muscle and joint pathology in a mouse model of PTOA. Four‐month‐old mice were randomized to receive a CSF1‐R inhibitor and studied for 7 or 28 days after joint injury. Additionally, we profiled synovial fluid samples for CSF1‐R from patients with injury to their ACL. Transcriptomic analysis of quadriceps muscle and articular cartilage in CSF1‐R inhibitor‐treated animals at 7 days after injury revealed elevated chondrocyte differentiation within articular cartilage and enhanced metabolic and contractile gene expression within skeletal muscle. At 28 days post‐injury, CSF1‐R inhibition attenuated PTOA severity and mitigated skeletal muscle atrophy. Patient synovial fluid CSF1‐R levels correlated with matrix metalloproteinase 13, a prognostic marker and molecular effector of PTOA. Our findings support an opportunity for CSF1‐R targeting to mitigate the severity of PTOA and muscle atrophy after joint injury.
imageKey points
Posttraumatic osteoarthritis (PTOA) of the knee commonly results from direct injury to the joint, which is characterized by pain, weakness, and disability.
Induction of colony stimulating factor one receptor (CSF1‐R) is positively associated with knee trauma severity, and the initial acute inflammatory state suppresses muscle recovery and degrades articular cartilage.
Skeletal muscle and articular cartilage transcriptomic response following direct joint injury in a murine model of PTOA is rescued by pharmacological inhibition of CSF1‐R.
CSF1‐R inhibition mitigated skeletal muscle atrophy and attenuated PTOA severity and synovitis.
Patient synovial fluid CSF1‐R levels correlated with matrix metalloproteinase 13, a prognostic marker and molecular effector of PTOA, offering further evidence for CSF1‐R as a therapeutic target across musculoskeletal tissues after injury.
Design of Soluble Compounds for Optical Control of Tetrameric P-Loop Ion Channels
Authors: Gataulina E. D. , Nikolaev M. V. , Tikhonov D. B. 
Published: December 2024

Abstract
The photopharmacology of ion channels is a rapidly developing
area. One reason for paying special attention to the design of photoswitchable
ligands for ion channels is that the channels control fast neurophysiological
processes, and their ability to be rapidly and locally modulated
by light is very important. In the photopharmacology of ion channels,
existing drugs are usually employed to design photochromic analogs
through the incorporation of a photochromic moiety. At present,
photochromic compounds are designed for many channels belong to
the superfamily of so-called P-loop channels. Photoswitchable drugs
differ in their activities, photosensitivity, selectivity, and mechanisms
of action. This paper provides an overview of the available compounds
and the design of their structures.
Late onset of Pompe’s disease: a rare cause of respiratory failure
Authors: Krogh Charlotte Loumann , Lauritsen Anne Øberg , Hägi-Pedersen Daniel 
Published: December 2024

A woman in her 30s was admitted acutely to the emergency department with severe respiratory failure of unknown cause. On arrival, the patient was severely compromised with a reduced Glasgow Coma scale and arterial pCO2 > 14 kPa. She was intubated and taken to the intensive care unit for respiratory support and further investigations.A detailed patient history revealed multiple admissions in the psychiatric sector with anxiety disorder, as well as numerous admissions to the emergency department with progressive tremors, muscle fatigue and dyspnoea, all of which were interpreted as anxiety attacks. Based on this history, we suspected a neurological disorder in our patient. Following extensive neurological investigation and additional genetic studies, the diagnosis of late-onset Pompe’s disease was confirmed.Late-onset Pompe’s disease can pose a diagnostic challenge due to its multifaceted presentation with non-specific symptoms. Any patient not responding to treatment should urge clinicians to re-evaluate their differential diagnoses.
Methaemoglobinemia in paediatrics: a complex diagnostic journey
Authors: Aires Martins Inês , Carvalho Queirós Joana , Cleto Esmeralda , Barbosa Telma 
Published: December 2024

Methaemoglobinemia is a potentially life-threatening condition in which the heme iron is oxidised from the ferrous (Fe2+) to the ferric (Fe3+) state, leading to a left shift of the oxygen-dissociation curve and impaired oxygen delivery to the tissues. It can be either acquired through exposure to certain drugs or chemicals or inherited due to genetic mutations affecting enzymes like cytochrome b5 reductase.We report a case of an adolescent who presented with a persistent cough and unremarkable physical examination, making diagnosis challenging. A low SpO2level that did not improve with supplemental oxygen prompted further testing, leading to the diagnosis of methaemoglobinemia caused by cytochrome b5 reductase deficiency. The case highlights the complexities of diagnosing methaemoglobinemia, especially in the absence of classic symptoms like cyanosis. It underscores the importance of thorough clinical assessment in atypical cases and the need for heightened awareness of rare conditions.