bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mapping coral calcification strategies from in situ boron isotope and trace element measurements of the tropical coral Siderastrea siderea
Authors: Chalk T. B. , Standish C. D. , D’Angelo C. , Castillo K. D. , Milton J. A. , Foster G. L. 
Published: January 2021

AbstractBoron isotopic and elemental analysis of coral aragonite can give important insights into the calcification strategies employed in coral skeletal construction. Traditional methods of analysis have limited spatial (and thus temporal) resolution, hindering attempts to unravel skeletal heterogeneity. Laser ablation mass spectrometry allows a much more refined view, and here we employ these techniques to explore boron isotope and co-varying elemental ratios in the tropical coral Siderastrea siderea. We generate two-dimensional maps of the carbonate parameters within the calcification medium that deposited the skeleton, which reveal large heterogeneities in carbonate chemistry across the macro-structure of a coral polyp. These differences have the potential to bias proxy interpretations, and indicate that different processes facilitated precipitation of different parts of the coral skeleton: the low-density columella being precipitated from a fluid with a carbonate composition closer to seawater, compared to the high-density inter-polyp walls where aragonite saturation was ~ 5 times that of external seawater. Therefore, the skeleton does not precipitate from a spatially homogeneous fluid and its different parts may thus have varying sensitivity to environmental stress. This offers new insights into the mechanisms behind the response of the S. siderea skeletal phenotype to ocean acidification.
Chemotaxis and swarming in differentiated HL-60 neutrophil-like cells
Authors: Babatunde Kehinde Adebayo , Wang Xiao , Hopke Alex , Lannes Nils , Mantel Pierre-Yves , Irimia Daniel 
Published: January 2021

AbstractThe human leukemia cell line (HL-60) is an alternative to primary neutrophils in research studies. However, because HL-60 cells proliferate in an incompletely differentiated state, they must undergo differentiation before they acquire the functional properties of neutrophils. Here we provide evidence of swarming and chemotaxis in differentiated HL-60 neutrophil-like cells (dHL-60) using precise microfluidic assays. We found that dimethyl sulfoxide differentiated HL-60 cells (DdHL-60) have a larger size, increased length, and lower ability to squeeze through narrow channels compared to primary neutrophils. They migrate through tapered microfluidic channels slower than primary neutrophils, but faster than HL-60s differentiated by other protocols, e.g., using all-trans retinoic acid. We found that dHL-60 can swarm toward zymosan particle clusters, though they display disorganized migratory patterns and produce swarms of smaller size compared to primary neutrophils.
Quantitative trait loci and transcriptome signatures associated with avian heritable resistance to Campylobacter
Authors: Psifidi Androniki , Kranis Andreas , Rothwell Lisa M. , Bremner Abi , Russell Kay , Robledo Diego , Bush Stephen J. , Fife Mark , Hocking Paul M. , Banos Georgios , Hume David A. , Kaufman Jim , Bailey Richard A. , Avendano Santiago , Watson Kellie A. , Kaiser Pete , Stevens Mark. P. 
Published: January 2021

AbstractCampylobacter is the leading cause of bacterial foodborne gastroenteritis worldwide. Handling or consumption of contaminated poultry meat is a key risk factor for human campylobacteriosis. One potential control strategy is to select poultry with increased resistance to Campylobacter. We associated high-density genome-wide genotypes (600K single nucleotide polymorphisms) of 3000 commercial broilers with Campylobacter load in their caeca. Trait heritability was modest but significant (h2 = 0.11 ± 0.03). Results confirmed quantitative trait loci (QTL) on chromosomes 14 and 16 previously identified in inbred chicken lines, and detected two additional QTLs on chromosomes 19 and 26. RNA-Seq analysis of broilers at the extremes of colonisation phenotype identified differentially transcribed genes within the QTL on chromosome 16 and proximal to the major histocompatibility complex (MHC) locus. We identified strong cis-QTLs located within MHC suggesting the presence of cis-acting variation in MHC class I and II and BG genes. Pathway and network analyses implicated cooperative functional pathways and networks in colonisation, including those related to antigen presentation, innate and adaptive immune responses, calcium, and renin–angiotensin signalling. While co-selection for enhanced resistance and other breeding goals is feasible, the frequency of resistance-associated alleles was high in the population studied and non-genetic factors significantly influenced Campylobacter colonisation.
Discovery of annual growth in a modern olive branch based on carbon isotopes and implications for the Bronze Age volcanic eruption of Santorini
Authors: Ehrlich Yael , Regev Lior , Boaretto Elisabetta 
Published: January 2021

AbstractThe volcanic eruption of Santorini in the Bronze Age left detectable debris across the Mediterranean, serving as an anchor in time for the region, synchronizing chronologies of different sites. However, dating the eruption has been elusive for decades, as radiocarbon indicates a date about a century earlier than archaeological chronologies. The identification of annual rings by CT in a charred olive branch, buried alive beneath the tephra on Santorini, was key in radiocarbon dating the eruption. Here, we detect a verified annual growth in a modern olive branch for the first time, using stable isotope analysis and high-resolution radiocarbon dating, identifying down to the growing season in some years. The verified growth is largely visible by CT, both in the branch’s fresh and charred forms. Although these results support the validity of the Santorini branch date, we observed some chronological anomalies in modern olive and simulated possible date range scenarios of the volcanic eruption of Santorini, given these observed phenomena. The results offer a way to reconcile this long-standing debate towards a mid-sixteenth century BCE date.
Prioritizing conservation actions in urbanizing landscapes
Authors: Ettinger A. K. , Buhle E. R. , Feist B. E. , Howe E. , Spromberg J. A. , Scholz N. L. , Levin P. S. 
Published: January 2021

AbstractUrbanization-driven landscape changes are harmful to many species. Negative effects can be mitigated through habitat preservation and restoration, but it is often difficult to prioritize these conservation actions. This is due, in part, to the scarcity of species response data, which limit the predictive accuracy of modeling to estimate critical thresholds for biological decline and recovery. To address these challenges, we quantify effort required for restoration, in combination with a clear conservation objective and associated metric (e.g., habitat for focal organisms). We develop and apply this framework to coho salmon (Oncorhynchus kisutch), a highly migratory and culturally iconic species in western North America that is particularly sensitive to urbanization. We examine how uncertainty in biological parameters may alter locations prioritized for conservation action and compare this to the effect of shifting to a different conservation metric (e.g., a different focal salmon species). Our approach prioritized suburban areas (those with intermediate urbanization effects) for preservation and restoration action to benefit coho. We found that prioritization was most sensitive to the selected metric, rather than the level of uncertainty or critical threshold values. Our analyses highlight the importance of identifying metrics that are well-aligned with intended outcomes.
Trends in molecular characteristics and antimicrobial resistance of group B streptococci: a multicenter study in Serbia, 2015–2020
Authors: Kekic Dusan , Gajic Ina , Opavski Natasa , Kojic Milan , Vukotic Goran , Smitran Aleksandra , Boskovic Lidija , Stojkovic Marina , Ranin Lazar 
Published: January 2021

AbstractGroup B Streptococcus (GBS) is a major cause of neonatal morbidity and mortality. Serbia has not fully implemented preventive measures against GBS neonatal diseases. Therefore, we aimed to assess the maternal GBS colonisation and invasive neonatal disease rate, to reveal the trends of antimicrobial resistance and serotype distribution of GBS from various patient groups. Randomly selected non-invasive (n = 991) and all invasive GBS (n = 80) collected throughout Serbia from 2015 to 2020 were tested for antimicrobial susceptibility, capsular typing, and hvgA detection. Overall, 877/5621 (15.6%) pregnant women were colonised with GBS. Invasive GBS infections incidence in infants (0.18/1000 live births) showed a decreasing trend (0.3 to 0.1/1000 live births). Type III was overrepresented in infants with invasive infections (n = 35, 58.3%), whereas type V predominated among colonised adults (n = 224, 25.5%) and those with noninvasive (n = 37, 32.5%) and invasive infections (n = 8, 40%). The hypervirulent clone III/ST17 was highly associated with invasive infections (n = 28, 35%), particularly late-onset disease (n = 9, 47.4%), showing an increase from 12.3 to 14.8%. The GBS resistance to erythromycin and clindamycin was 26.7% and 22.1%, respectively, with an upward trend. The emergence of the hypervirulent clone III/ST17 and the escalation in GBS resistance highlight an urgent need for continuous monitoring of GBS infections.
Population codes of prior knowledge learned through environmental regularities
Authors: Quax Silvan C. , Bosch Sander E. , Peelen Marius V. , van Gerven Marcel A. J. 
Published: January 2021

AbstractHow the brain makes correct inferences about its environment based on noisy and ambiguous observations is one of the fundamental questions in Neuroscience. Prior knowledge about the probability with which certain events occur in the environment plays an important role in this process. Humans are able to incorporate such prior knowledge in an efficient, Bayes optimal, way in many situations, but it remains an open question how the brain acquires and represents this prior knowledge. The long time spans over which prior knowledge is acquired make it a challenging question to investigate experimentally. In order to guide future experiments with clear empirical predictions, we used a neural network model to learn two commonly used tasks in the experimental literature (i.e. orientation classification and orientation estimation) where the prior probability of observing a certain stimulus is manipulated. We show that a population of neurons learns to correctly represent and incorporate prior knowledge, by only receiving feedback about the accuracy of their inference from trial-to-trial and without any probabilistic feedback. We identify different factors that can influence the neural responses to unexpected or expected stimuli, and find a novel mechanism that changes the activation threshold of neurons, depending on the prior probability of the encoded stimulus. In a task where estimating the exact stimulus value is important, more likely stimuli also led to denser tuning curve distributions and narrower tuning curves, allocating computational resources such that information processing is enhanced for more likely stimuli. These results can explain several different experimental findings, clarify why some contradicting observations concerning the neural responses to expected versus unexpected stimuli have been reported and pose some clear and testable predictions about the neural representation of prior knowledge that can guide future experiments.
Persistent meanders and eddies lead to quasi-steady Lagrangian transport patterns in a weak western boundary current
Authors: Gouveia M. B. , Duran R. , Lorenzzetti J. A. , Assireu A. T. , Toste R. , de F. Assad L. P. , Gherardi D. F. M. 
Published: January 2021

AbstractThe Brazil Current (BC) is a weak western boundary current flowing along the Southwestern Atlantic Ocean. It is frequently described as a flow with intense mesoscale activity and relatively low volume transport between 5.0 and 10.0 Sv. We use a 13-year eddy-resolving primitive-equation simulation to show that the presence of persistent meanders and eddies leads to characteristic quasi-steady Lagrangian transport patterns, aptly extracted through climatological Lagrangian Coherent Structures (cLCS). The cLCS position the surface expression of the BC core along the 2000-m isobath, in excellent visual agreement with high resolution satellite sea-surface temperature and the model Eulerian mean velocity. The cLCS deformation pattern also responds to zonally persistent cross-shelf SSH transition from positive (high) values near coastline to low (negative) values between 200- and 2000-m and back to positive (high) offshore from the 2000-m isobath. Zonally-paired cyclonic and anticyclonic structures are embedded in this transition, also causing the cLCS to deform into chevrons. An efficient transport barrier is identified close to the 200-m isobath confirmed by limited inshore movement of drogued drifters and accurately indicated by an along slope maxima of climatological strength of attraction. We also show that the persistent cyclonic and anticyclonic structures may induce localized cross-shelf transport. Regions of low climatological strength of attraction coincide with large shelves and with stagnant synthetic trajectories. We also show that cLCS accurately depict trajectories initiated at the location of Chevron’s spill (November 2011) as compared to synthetic and satellite-tracked trajectories, and the outline of the oil from that accident. There is also an agreement between the large-scale oil slicks reaching the Brazilian beaches (from August 2019 to February 2020) and the strength of climatological attraction at the coast. Our work also clarifies the influence of persistent mesoscale structures on the regional circulation. The identification and quantitative description of climatological Lagrangian coherent structures is expected to improve the effectiveness of future emergency response to oil spills, contingency planning, rescue operations, larval and fish connectivity assessment, drifter launch strategies, waste pollutant and marine debris dispersion and destination.
Anticancer potential of rhizome extract and a labdane diterpenoid from Curcuma mutabilis plant endemic to Western Ghats of India
Authors: Soumya T. , Lakshmipriya T. , Klika Karel D. , Jayasree P. R. , Manish Kumar P. R. 
Published: January 2021

AbstractZingiberaceae plants are well known for their use in ethnomedicine. Curcuma mutabilis Škorničk., M. Sabu & Prasanthk., is an endemic Zingiberaceae species from Western Ghats of Kerala, India. Here, we report for the first time, the anticancer potential of petroleum ether extract from C. mutabilis rhizome (CMRP) and a novel labdane diterpenoid, (E)-14, 15-epoxylabda-8(17), 12-dien-16-al (Cm epoxide) isolated from it. CMRP was found to be a mixture of potent bioactive compounds including Cm epoxide. Both the extract and the compound displayed superior antiproliferative activity against several human cancer cell lines, without any display of cytotoxicity towards normal human cells such as peripheral blood derived lymphocytes and erythrocytes. CMRP treatment resulted in phosphatidylserine externalization, increase in the levels of intracellular ROS, Ca2+, loss of mitochondrial membrane potential as well as fragmentation of genomic DNA. Analyses of transcript profiling and immunostained western blots of extract-treated cancer cells confirmed induction of apoptosis by both intrinsic and extrinsic pathways. The purified compound, Cm epoxide, was also found to induce apoptosis in many human cancer cell types tested. Both CMRP and the Cm epoxide were found to be pharmacologically safe in terms of acute toxicity assessment using Swiss albino mice model. Further, molecular docking interactions of Cm epoxide with selected proteins involved in cell survival and death were also indicative of its druggability. Overall, our findings reveal that the endemic C. mutabilis rhizome extract and the compound Cm epoxide isolated from it are potential candidates for development of future cancer chemotherapeutics.
Newborns’ sensitivity to speed changes as a building block for animacy perception
Authors: Di Giorgio Elisa , Lunghi Marco , Vallortigara Giorgio , Simion Francesca 
Published: January 2021

AbstractThe human visual system can discriminate between animate beings vs. inanimate objects on the basis of some kinematic cues, such as starting from rest and speed changes by self-propulsion. The ontogenetic origin of such capability is still under debate. Here we investigate for the first time whether newborns manifest an attentional bias toward objects that abruptly change their speed along a trajectory as contrasted with objects that move at a constant speed. To this end, we systematically manipulated the motion speed of two objects. An object that moves with a constant speed was contrasted with an object that suddenly increases (Experiment 1) or with one that suddenly decreases its speed (Experiment 2). When presented with a single speed change, newborns did not show any visual preference. However, newborns preferred an object that abruptly increases and then decreases its speed (Experiment 3),but they did not show any visual preference for the reverse sequence pattern (Experiment 4). Overall, results are discussed in line with the hypothesis of the existence of attentional biases in newborns that trigger their attention towards some visual cues of motion that characterized animate perception in adults.
5G as a wireless power grid
Authors: Eid Aline , Hester Jimmy G. D. , Tentzeris Manos M. 
Published: January 2021

Abstract5G has been designed for blazing fast and low-latency communications. To do so, mm-wave frequencies were adopted and allowed unprecedently high radiated power densities by the FCC. Unknowingly, the architects of 5G have, thereby, created a wireless power grid capable of powering devices at ranges far exceeding the capabilities of any existing technologies. However, this potential could only be realized if a fundamental trade-off in wireless energy harvesting could be circumvented. Here, we propose a solution that breaks the usual paradigm, imprisoned in the trade-off between rectenna angular coverage and turn-on sensitivity. The concept relies on the implementation of a Rotman lens between the antennas and the rectifiers. The printed, flexible mm-wave lens allows robust and bending-resilient operation over more than 20 GHz of gain and angular bandwidths. Antenna sub-arrays, rectifiers and DC combiners are then added to the structure to demonstrate its combination of large angular coverage and turn-on sensitivity—in both planar and bent conditions—and a harvesting ability up to a distance of 2.83 m in its current configuration and exceeding 180 m using state-of-the-art rectifiers enabling the harvesting of several μW of DC power (around 6 μW at 180 m with 75 dBm EIRP).
Immune-tolerance to human iPS-derived neural progenitors xenografted into the immature cerebellum is overridden by species-specific differences in differentiation timing
Authors: Nato Giulia , Corti Alessandro , Parmigiani Elena , Jachetti Elena , Lecis Daniele , Colombo Mario Paolo , Delia Domenico , Buffo Annalisa , Magrassi Lorenzo 
Published: January 2021

AbstractWe xeno-transplanted human neural precursor cells derived from induced pluripotent stem cells into the cerebellum and brainstem of mice and rats during prenatal development or the first postnatal week. The transplants survived and started to differentiate up to 1 month after birth when they were rejected by both species. Extended survival and differentiation of the same cells were obtained only when they were transplanted in NOD-SCID mice. Transplants of human neural precursor cells mixed with the same cells after partial in vitro differentiation or with a cellular extract obtained from adult rat cerebellum increased survival of the xeno-graft beyond one month. These findings are consistent with the hypothesis that the slower pace of differentiation of human neural precursors compared to that of rodents restricts induction of immune-tolerance to human antigens expressed before completion of maturation of the immune system. With further maturation the transplanted neural precursors expressed more mature antigens before the graft were rejected. Supplementation of the immature cells suspensions with more mature antigens may help to induce immune-tolerance for those antigens expressed only later by the engrafted cells.
Novel CFD modeling approaches to assessing urine flow in prostatic urethra after transurethral surgery
Authors: Zhang Bin , Liu Shuang , Liu Yinxia , Wu Bo , Zhang Xuhui , Wang Xin , Liang Xuezhi , Cao Xiaoming , Wang Dongwen , Wu Chin-Lee 
Published: January 2021

AbstractAssessment of the pressure and velocity of urine flow for different diameter ratios of prostatic urethra (RPU) after transurethral surgery using computational fluid dynamics (CFD). A standardized and idealized two-dimensional CFD model after transurethral surgery (CATS-1st) was developed for post-surgery mid-voiding. Using CATS-1st, 210 examples were amplified according to an array of size [3][5][14], which contained three groups of longitudinal diameters of prostatic urethra (LD-PU). Each of these groups contained five subgroups of transverse diameters of the bladder neck (TD-BN), each with 14 examples of transverse diameters of PU (TD-PU). The pressure and velocity of urine flow were monitored through flow dynamics simulation, and the relationship among RPU-1 (TD-PU/TD-BN), RPU-2 (RPU-1/LD-PU), the transverse diameter of the vortex, and the midpoint velocity of the external urethral orifice (MV-EUO) was determined. A total of 210 CATS examples, including CATS-1st examples, were analyzed. High (bladder and PU) and medium/low (the rest of the urethra) pressure zones, and low (bladder), medium (PU), and high (the rest of the urethra) velocity zones were determined. The rapid changes in the velocity were concentrated in and around the PU. Laminar flow was present in all the examples. The vortices appeared and then gradually shrank with reducing RPU on both the sides of PU in 182 examples. In the vortex examples, minimum RPU-1 and RPU-2 reached close to the values of 0.79 and 0.02, respectively. MV-EUO increased gradually with decreasing RPU. In comparison to the vortex examples, the non-vortex examples exhibited a significantly higher (p < 0.01) MV-EUO. The developed CFD models (CATS) presented an effective simulation of urine flow behavior within the PU after transurethral surgery for benign prostatic hyperplasia (BPH). These models could prove to be useful for morphological repair in PU after transurethral surgery.