bluuebunny/crossref_metadata_2025_split huggingface dataset:
Genetic Analysis for Mapping of Physiological Traits Related to Seed Vigour in Rice Through Association Mapping
Authors: Sanghamitra Priyadarshini , nayak Deepak Kumar , Barik saumya ranjan , Pandit Elssa , Bastia Ramakrushna , Mohanty Shakti Prakash , Mishra Jyotirmayee , Behera Abhisarika , Sahoo Auromeera , Patttanaik Suchismita , Moharana Arpita , samal Debabrata , Pradhan Sharat Kumar 
Published: March 2021

Abstract
Background:Good quality seed is an important factor for a good crop. Vigor is the key performing trait of quality seed. Genomic regions controlling the physiological traits related to seed vigour are not fully reported.Results: A panel was prepared by including genotypes from all the groups of the fifteen physiological traits representing a population of 250 germplasm lines. Wide variations were observed for the 15 physiological traits in the population. The population was classified into 6 genetic groups. Presence of linkage disequilibrium (LD) was detected in the panel population based on the fixation indices of the subpopulations. Moderate values of gene diversity, polymorphic information content (PIC) and other diversity parameters were estimated from the population by genotyping with 109 SSR markers. The population was classified into subpopulations and sub-clusters showed relation with the genotypes for their physiological traits. Ten physiological traits were detected to be significantly associated with SSR markers analyzed by both the General linear model (GLM) and Mixed linear model (MLM). A total of 19 novel QTLs controlling different physiological traits namely qGP 6.2 and qGP 8.2 for germination % (GP); qGR 9.1 for germination rate (GR); qGI 1.2, qGI 6.1, qGI 8.1, qGI 11.1 and qGI 12.1 for germination index (GI); qRPE1.1 and qRPE8.1 for rate of plumule elongation (RPE); qAGR 8.1 for AGR; qRSG1.2 and qRSG12.2 for rate of shoot growth (RSG); qRGR3.1 and qRGR11.1 for relative growth rate (RGR); qSVI 1.1 for seed vigour index I (SVI I); qSVII 1.1, qSVII3.1, and qSVII12.1 for seed vigour index II (SVI II), and qRRG8.1 for relative root growth rate were identified. The reported QTL for root length, qRL11.1 was validated in this mapping population. Additionally, QTLs, qRPE8.1 and qAGR8.1 of traits, RPE and AGR; qGI 6.1 and qGP6.2 of GI and GP; qGI 11.1 and qRL11.1 of GI and RL; qAGR8.1 and qRRG8.1 of AGR and RRG, and qRSG3.1 and qRGR3.1of traits RSG and RGR were detected for co-localization or co-inheritance. Conclusion: The traits identified and markers detected in the association analysis will be useful for improvement of seed vigour trait through marker-assisted selection in rice.
Significance of Uranium Series Disequilibrium and Their Radiological Impacts In Abu Garadi Altered Granites.
Authors: Hussein Mahmoud A. , khattab Mahmoud , El-Said Waleed A. , Abed Neveen S. , Tawfic A. F. 
Published: March 2021

Abstract
Radioactivity of U, Th series and 40K in the collected samples from Abu Garadi area were measured using Hyper Pure Germanium detector Gamma spectrometer (HPGe). 232Th, 226Ra, 40K and 238U contents were determined in the different samples that indicating high uranium high thorium type of these granites. The activity concentrations of 232Th, 238U, 234U/238U activity ratio of the studied altered granites ranged between 1.98 to 4.25 with 2.89 as an average, indicating that the samples passed from the incipience of the oxidation-reduction zone (234U/238U=1.98) to the reduction zone (234U/238U= 4.52). 238U/235U activity ratios showed broad range as a result of the alteration processes. 226Ra and 40K are very important in determination of different environmental hazard impacts. The activity concentrations average of 238U, 232Th, 226Ra and 40K were 6553.7 ± 3.1 Bq kg− 1, 3944 ± 0.9 Bq kg− 1 201.3 ± 1.1 Bq kg− 1 and 619.4 ± 0.02 Bq kg− 1. The absorbed gamma dose rate (D), external hazard index (Hex), annual effective dose rate equivalent, radium equivalent (Raeq), internal hazard index (Hin), gamma index (Iγ) as well as Excess Lifetime Cancer Risk (ELCR) were applied based on the measured radionuclide concentration of the 238U, 232Th, 226Ra and 40K.
Analysis of Genomes Changes in Pseudomonas Chlororaphis Subsp. Aurantiaca Strains Producing Phenazines
Authors: Liaudanskaya Anastasia I. , Maximova Natalia P. , Verameyenka Katsiaryna G. 
Published: March 2021

Abstract
Genomes of three strains – phenazine producers – Pseudomonas chlororaphis subsp. aurantiaca (B-162 (wild-type), mutant strain B-162/255 and its derivatives B-162/17) were sequenced and compared. All genome annotations revealed 6347 CDS, 5 rRNA clusters (5S, 16S, 23S) and 59 tRNA genes. Comparison analysis of wild-type strain and B-162/255 mutant strain genomes allowed revealing 32 mutations. 19 new mutations were detected upon comparison of genomes strains B-162/255 and B-162/17. Further bioinformatics analysis allowed predicting mutant proteins` functions and secondary structures of five gene products, mutations in which might potentially have influence on phenazine synthesis and secretion in Pseudomonas bacteria. These genes are phenylalanine hydroxylase transcriptional activator PhhR, type I secretion system ATPase, transcriptional regulator MvaT, GacA response regulator and histidine kinase. Amino acid substitutions were located in domain structures of corresponding proteins.
Age affects pigeon memory capacity, but not representation of serial order, during a locomotor sequential-learning task
Authors: Meier Christina , Sepehri Parisa , Kelly Debbie M. 
Published: March 2021

Abstract
Aging affects individuals of every species, with sometimes detrimental effects on memory and cognition. The simultaneous-chaining task, a sequential-learning task, requires subjects to select items in a predetermined sequence, putting demands on memory and cognitive processing capacity. It is thus a useful tool to investigate age-related differences in these domains. Pigeons of three age groups (young, adult and aged) completed a locomotor adaptation of the task, learning a list of four items. Training began presenting only the first item; additional items were added, one at a time, once previous items were reliably selected in their correct order. Although memory capacity declined noticeably with age, not all aged pigeons showed impairments compared to younger pigeons, suggesting that inter-individual variability emerged with age. During a subsequent free-recall memory test, when all trained items were presented alongside novel distractor items, most pigeons did not reproduce the trained sequence in the absence of reinforcement. During a further forced-choice test, when pigeons were given a choice between only two of the trained items, all three age groups showed evidence of an understanding of the ordinal relationship between items by choosing the earlier item, indicating that complex cognitive processing, unlike memory capacity, remained unaffected by age.
Curcumin Protects HepG2 Cells from the Cytotoxic Effect of Drugs with Anti-fibrotic Activity
Authors: Medina-Pizaño Mariana Y. , Medina-Rosales Marina N. , Sánchez-Alemán Esperanza , Martínez-Hernández Sandra L. , Aldaba-Muruato Liseth R. , Ventura-Juarez Javier , Muñoz-Ortega Martin Humberto 
Published: March 2021

Abstract
Background: The α and β adrenoblockers have been tested as an alternative treatment for chronic liver lesions such as fibrosis and cirrhosis in animal models, as well as their possible participation during the regeneration of the damage caused by liver cirrhosis in a hamster model. However, it was observed that doxazosin caused slight morphological changes in hepatocytes, while that curcumin showed protection to the hepatic parenchyma. Regardless, the pharmacokinetic effects of these 𝛼/𝛽 adrenoblockers on the hepatocytes' cell viability, possibly involved in the hepatic parenchyma's repopulation during cirrhosis reversal, are unknown. The present study aimed to elucidate the protective effect of curcumin on the possible side effects of doxazosin, tamsulosin, and carvedilol on the HepG2 cell line, drugs already tested with antifibrotic activity.Methods: HepG2 cells were exposed to 0.1, 0.5, 10, and 25 µM of doxazosin, carvedilol, and tamsulosin for 24, 48, and 72 h, for curcumin, cells were pretreated with 1 µM for 1 h before exposure to α and β adrenoblockers. The cell viability was assessed by MTT assay. The morphological changes were determined using hematoxylin and eosin (H&E) staining, scanning electron microscope (SEM), and acridine orange (AO) staining. Results: We observed that the doxazosin decreases cell viability dependently time and dose; carvedilol and tamsulosin increase cell proliferation. However, curcumin induces regulation of these effects in HepG2 cell line, increasing or maintaining viability compared to control. The pretreatment with curcumin regulated AST levels (aspartate aminotransferase) and ALT (alanine aminotransferase) in cells exposed to α and β adrenoblockers. The SEM and H&E staining provided evidence that doxazosin, carvedilol, and tamsulosin induced morphological changes in HepG2 cell line, depending on time and dose, approximately 80% of the cells treated with drugs were balonized, and curcumin protected these effects, maintaining the morphology in 90% of the treated cells.Conclusions: The present study demonstrates that curcumin protected the HepG2 cells against cytotoxicity and morphological changes induced by the α and β adrenoblockers attenuating secondary effects for possible oxidative stress. In this way, it is concluded that these treatments with antifibrotic effect, in co-treatment with curcumin, will not affect the possible repopulation process of the liver parenchyma during the reversion of fibrosis.
A web-based survey finds low antiretroviral therapy adherence in approximately 35% of Japanese people living with HIV
Authors: Inoue Yoji , Oka Shinichi , Yokoyama Seiji , Hasegawa Koichi , Mahlich Joerg , Schaede Ulrike , Habuka Noriyuki , Murata Yoko 
Published: March 2021

Abstract
The authors have requested that this preprint be removed from Research Square.
Protective effects of aerobic exercise on survival, MOSD, and blood glucose response during sepsis
Authors: Wang Xishuai , Wang Zhiqing , Tang Donghui 
Published: March 2021

Abstract
Aim: In the present study, we attempted to investigate whether aerobic exercise could prevent sepsis and its complications and explored the related mechanisms. Methods: Forty ICR mice were divided into four groups: control (Con), LPS, exercise (Ex), and exercise + LPS (Ex + LPS). Low-intensity aerobic exercise was performed for 4 weeks. Forty-eight hours after the last exercise intervention, LPS and Ex+LPS mice received 5 mg/kg LPS intraperitoneally for induction of sepsis. This study examined the effects of a 4-week exercise pretreatment on LPS-induced changes in blood glucose (BG) levels; inflammatory cytokine levels in BALF; bronchoalveolar lavage fluid (BALF) cell counts; the degree of pulmonary edema; neutrophil density in liver, lung, and kidney tissues; and the gene expression levels of IL-1RN, IL-8, IL-10, TNF-α, Sirt-1, and Nrf-2 in lung tissue. Sixty mice were used to perform survival rate analysis. Results: A 4-week exercise pretreatment significantly reversed the histopathological severity of LPS-induced lung, heart, liver, kidney, and aortic injuries (P < 0.05) and ameliorated neutrophil inflammation in the lung, heart, liver, and kidney (P < 0.05). A 4-week exercise pretreatment improved survival rates (P < 0.01), deranged glucose homeostasis (P < 0.01), and pulmonary edema (P < 0.01) in mice with sepsis. A 4-week exercise pretreatment increased the levels of IL-6 (P < 0.05), IL-10 (P < 0.05), and IL-1RN (P < 0.05) and decreased the levels of IL-8 (P < 0.05) and TNF-α (P < 0.05) in BALF. A 4-week exercise pretreatment decreased the gene expression levels of IL-8 (P < 0.01) and TNF-α (P < 0.01) and increased the gene expression levels of IL-1RN (P < 0.01) and IL-10 (P < 0.01) in mice with sepsis. 4-week exercise pretreatment activated the gene expression levels of lung protective factors Sirt-1 (P < 0.01) and Nrf-2 (P < 0.01). Conclusion: Aerobic exercise improved survival rates, multiple organ dysfunction syndromes (MODS), and deranged glucose homeostasis in mice with sepsis. Aerobic exercise alleviated lung injury partly because aerobic exercise exerted immune effects and activated Sirt-1/Nrf-2 signaling.
Assessment of Quality Management System for Clinical Nutrition in Jiangsu
Authors: Wang Jin , Ma Xianghua , Pan Chen 
Published: March 2021

Abstract
Objective: To critically evaluate the Quality Management System (QMS) for Clinical Nutrition (CN) in Jiangsu. Monitor its performance in quality assessment as well as human resource management from nutrition aspect. Investigate the appliance and development of Artificial Intelligence (AI) in medical quality control.Data Source: The study source of this research was all the staffs of 70 Clinical Nutrition Department (CND) of the tertiary hospitals in Jiangsu Province, China. These departments are all members of the Quality Management System of Clinical Nutrition in Jiangsu (QMSNJ).Methods: An online survey was conducted on all 341 employees within all these CNDs based on the staff information from the surveyed medical institutions. The questionnaire contains 5 aspects, while data analysis and AI evaluation were focused on human resource information. Results: 330 questionnaires were collected with the respondent rate of 96.77%. The QMS for CN has been build up for CNDs in Jiangsu, which achieved its target in human resource improvements, especially among dietitians. The increasing number of participated departments (42.8%) and the significant growth of dietitians (p=0.02, t=-0.42) are all expressions of the advancements of QMSNJ.Conclusion: As the first innovation of an online platform for QM in Jiangsu, JPCNMP has been successfully implemented among QMS from this research. This multidimensional electronic system can help QMSNJ and CND achieve quality assessment from various aspects, so as to realize the continuous improvement of clinical nutrition. The instrument of online platform, as well as AI technology for quality assessment is worth to be recommended and promoted in the future.
Non-contact, in-vivo, functional, and structural ophthalmic imaging using multimodal photoacoustic remote sensing (PARS) microscopy and swept source optical coherence tomography (SS-OCT)
Authors: Hosseinaee Zohreh , Abbasi Nima , Khalili Layla , Mukhangaliyeva Lyazzat , Pellegrino Nicholas , Reza Parsin Haji 
Published: March 2021

Abstract
Early diagnosis of ocular diseases improves the understanding of pathophysiology and aids in accurate monitoring and effective treatment. Advanced, multimodal ocular imaging platforms play a crucial role in visualization of ocular components and provide clinicians with a valuable tool for evaluating various eye diseases. Here, for the first time we present a non-contact, multiwavelength photoacoustic remote sensing (PARS) microscopy and swept-source optical coherence tomography (SS-OCT) for in-vivo functional and structural imaging of the eye. The system provides complementary imaging contrasts of optical absorption and optical scattering, and is used for non-contact, in-vivo imaging of murine eye. Results of vasculature and structural imaging as well as melanin content in the retinal pigment epithelium layer are presented. Multiwavelength PARS microscopy using Stimulated Raman Scattering is applied to enable in-vivo, non-contact oxygen saturation estimation in the ocular tissue. The reported work may be a major step towards clinical translation of ophthalmic technologies and has the potential to advance the diagnosis and treatment of ocular diseases.
Academic Stress and Suicidal Ideation: Moderated Mediation Study
Authors: Okechukwu Franca Obiageli , Ogba Kalu T. U. , Nwufo Juliet Ifeoma , Oluchi Ogba Miracle , Nneka Onyekachi Blessing , Nwanosike Chinonso , Amuche Onyishi Bonaventure 
Published: March 2021

Abstract
Objective: To examine the moderated mediation association of coping and resilience on academic stress and suicidal ideation. Method: We sample 505 undergraduate volunteers [including 329(65.1%) males and 176(34.9%) females] from three South-Eastern Nigerian Universities. Participants were administered self-report: Lakaev Academic Stress Response Scale (LASRS; Lakaev, 2006), Scale for Suicidal Ideation (SSI; Beck, Kovacs, & Weissman, 1979), Brief COPE (B-COPE; Carver, 1997) and Resilience Scale (RS-14; Wagnild & Young, 1993). Results: Hayes regression-based PROCESS macro showed that academic stress was a significant predictor of coping. Coping did not significantly predict suicidal ideation but moderated the effect of academic stress on suicidal ideation, such that low or moderate coping with academic stress would most likely lead to suicidal ideation. However, resilience negatively predicted suicidal ideation and was positively associated with academic stress. A Sobel test (z = 3.21, p = .004) confirmed that resilience fully mediated the relationship between academic stress and suicidal ideation.Conclusion: Educational administrators and policy makers should incorporate courses and teachings that entail effective coping skills as well as inculcate resilience especially to fresh undergraduates, since resilient students recover from academic stress given that they practice adequate coping strategies; and such students may not likely ideate about suicide
VitaminD3 Regulates T Cell Immune Responses in Allergen and Rhinovirus Induced Asthma
Authors: Finotto Susetta , Haag Patricia , Andreev Darja , Li Nina , Kiefer Alexander , Rauh Manfred , Gleich Luisa , Wirtz Stefan , Papadopoulos Nikolaos G. 
Published: March 2021

Abstract
Background: Serum 25(OH)-Vitamin D3 (VitD3) deficiency during infancy has been associated with asthma. The potential therapeutic role of VitD3 given in the airways and its interference with the allergen and Rhinovirus was the objective of this study. Methods: In two cohorts of children with and without asthma, serum levels of the C-reactive protein (CRP) were correlated to Serum VitD3 and in peripheral blood T cell inhibitor marker Programmed cell death protein 1 (PD1) mRNA was analyzed. In a murine model, VitD3 was given intranasally in vivo and in vitro to lung cells with allergen and Rhinovirus. Results: In the cohorts of pre-school age children without (control) asthma, CRP and VitD3 levels inversely correlated. In preschool asthmatic children that did not receive VitD3 supplementation as infant had more episode of asthma exacerbation associated with high CRP serum level. In peripheral blood cells from control but not asthmatic children with higher serum levels of VitD3 had lower PD1 mRNA levels. In murine model, OVA intranasal challenge induced Innate Lymphoid Cells type 2 (ILC2)-associated markers and Eosinophils in BALF and VitD3 inhibited lung inflammation and ILC2 markers. Furthermore, VitD3 given intranasally, induced CD4+T cells and reduced PD1, T regulatory cells in the lung. Similarly, VitD3 had a suppressive role on CD4+PD1+ T cells involved in T cell exhaustion in the airways in the absence of ST2 after Rhinovirus infection. Conclusion: These data support an inhibitory role of VitD3 on T cell exhaustion after allergen and rhinovirus infection that is relevant for pediatric asthma.
A promising cell line for research on the control of locust plagues
Authors: Watanabe Kazuyo , Sugahara Ryohei , Tanaka Seiji , Kayukawa Takumi 
Published: March 2021

Abstract
The desert locust (Schistocerca gregaria), which forms a huge swarm, consuming large amounts of wild and agricultural plants, is the most destructive migratory pest in the world. Recently, a large-scale locust plague occurred from Africa to South Asia. The main methods used to control these pests, which involve the use of inexpensive and highly residual insecticides, are raising concerns on their impact on humans, domestic animals, and the environment. Cell lines are a useful research tool in molecular biological analysis and drug discovery; however, no cell line is yet available for the research of S. gregaria. Here, we succeeded in establishing a cell line (Sg-155 cells) from S. gregaria embryos and validated its utility. Soaking with low concentrations of dsRNA induced high and long-lasting RNAi efficiency in Sg-155 cells. Furthermore, response to an insect hormone, a candidate target as an anti-locust agent, was observed at the gene expression level. Thus, the Sg-155 cell line is useful in exploring and evaluating target genes and could therefore be applied as a high-throughput screening tool in the development of anti-locust agents.
Effect of Lumbar Spinal Stenosis on Bone Mineral Density in Osteoporosis Patients Treated With Ibandronate
Authors: Park Hyung-Youl , Ha Ji-Yoon , Kim Ki-Won , Baek In-Hwa , Park Soo-Bin , Lee Jun-Seok 
Published: March 2021

Abstract
Background: Lumbar spinal stenosis (LSS) can cause various neurological symptoms and reduce the daily activity of patients. Many studies have shown that free physical activities and exercise can improve bone mineral density (BMD) in patients with osteoporosis. However, the effect of LSS on BMD has not been reported. The purpose of this study was to investigate the effects of LSS on BMD in patients treated with ibandronate for newly diagnosed osteoporosis.Methods: Group 1 included 83 patients with osteoporosis alone, and group 2 included 76 patients with both osteoporosis and symptomatic LSS. We confirmed four BMD values presented as T-score at initial, and 1-, 2-, and 3-year follow-ups. Mean BMD and annual changes of BMD for three years were compared between the two groups. Correlations between initial BMD and total change of BMD were also analyzed in both groups.Results: Mean annual BMDs were significantly higher in group 1 compared than in group 2 (-3.39 vs. -3.58 at 1-year; -3.27 vs. -3.49 at 2-year; -3.13 vs. -3.45 at 3-year; all p < 0.05). Annual change of BMD at 1-year follow-up (0.32 vs. 0.21, p = 0.036) and total change of BMD for three years (0.57 vs. 0.35, p = 0.002) were significantly higher in group 1. Group 1 had a strong negative correlation (r = -0.511, P = 0.000) between initial BMD and total change of BMD, whereas group 2 showed a weak negative correlation (r = -0.247, p = 0.032).Conclusions: Symptomatic LSS may interfere with BMD improvement in the treatment of osteoporosis with ibandronate. Active treatment for LSS with more potent treatment for osteoporosis should be taken to increase BMD for patients with osteoporosis and LSS.
Prevalence and Risk Factors for Bone Loss in Rheumatoid Arthritis Patients from South China: modeled by three methods
Authors: Hu Zhuoran , Zhang Lei , Lin Zhiming , Zhao Changlin , Xu Shuiming , Lin He , Zhang Jiejing , Li Wenjie , Chu Yongliang 
Published: March 2021

Abstract
Background. To explore the prevalence of bone loss among patients with rheumatoid arthritis and healthy controls and further explored the risk factors for osteopenia and osteoporosis of RA patients. Methods. A cross-sectional survey was undertaken in four hospitals in different districts in South China. Case records, laboratory tests, and bone mineral density (BMD) results were included. Multivariable logistic regression analysis, least absolute shrinkage, selection operator (LASSO), and random forest (RF) was for exploring the risk factors for osteopenia or osteoporosis in RA patients.Results. Four hundred five patients with RA and 198 HC were included. RA patients had lower BMD in almost detective sites than healthy controls; the decline of lumbar spine BMD was earlier than HC. RA patients were more likely to comorbid with osteopenia and osteoporosis (p for trend <0.001) in the lumbar spine than HC. Higher serum 25-hydroxyvitamin D3 level and using tumor necrosis factor inhibitor in the last year, aging, lower body mass index, and increased serum uric acid were associated with bone loss.Conclusions. RA patients were more prone and earlier to have bone loss than HC. More attention should be paid to measuring BMD in RA patients aging with lower BMI or hyperuricemia. Besides, serum vitamin D and all three detective sites are recommended to check routinely. TNFi usage in the last year might benefit bone mass.