bluuebunny/crossref_metadata_2025_split huggingface dataset:
Extracellular Vesicle-derived miR-320a Targets ZC3H12B to Inhibit Tumorigenesis, Invasion, and Angiogenesis in Ovarian Cancer
Authors: Huang Yan , Xu Midie , Jing Chuyu , Wu Xiaohua , Chen Xiaojun , Zhang Wei 
Published: September 2021

Abstract
Extracellular vesicles (EVs) play crucial roles in intercellular communication. miRNAs derived from EVs emerge as promising diagnostic indicators and therapeutic targets in a variety of malignancies. Tremendous studies have revealed the function of miRNAs derived from EVs in tumorigenesis, metastasis and other aspects. The mechanism of action of EV-derived miRNAs, however, in ovarian cancer remains largely unknown. In this study, EVs were enriched from the ovarian cancer cell lines. EVs as a whole could promote cell proliferation, invasion and new vasculature formation. However, the down-regulated EV-derived miR-320a was demonstrated to potentially suppress tumorigenesis, metastasis and angiogenesis. . Moreover, EV-derived miR-320a has been proved to directly regulate a previously unknown target, ZC3H12B. An unreported role of ZC3H12B in promoting ovarian cancer cell proliferation has been elucidated and miR-320a could mediate the expression of ZC3H12B, thereby inhibiting the downstream response. As for the practical clinic values, lower expression of EV-derived miR-320a correlates with shorter survival period, indicating that EV-derived miR-320a may also serve as a prognostic biomarker in ovarian cancer. This research provides new insight into the molecular mechanism of EV-derived miR-320a in ovarian cancer and may provide new therapeutic and prognostic strategies for ovarian cancer treatment.
Complete Mitogenome of Endangered and Endemic Nicobar Treeshrew (Tupaia Nicobarica) and Comparison with Other Scandentians
Authors: Kundu Shantanu , Pakrashi Avas , Kamalakannan Manokaran , Singha Devkant , Tyagi Kaomud , Venkatraman Chinnadurai , Kumar Vikas 
Published: September 2021

Abstract
The Nicobar treeshrew (Tupaia nicobarica) is an endangered smaller mammal endemic to the Nicobar Island of the Andaman Sea, India regarded as an alternative experimental animal model in biomedical research. The present study aimed to assemble the first mitochondrial genome of T. nicobarica to elucidate evolutionary relationship. The structure and variation of the novel mitochondrial genome were analyzed and compared with other Scandentians. The complete mitogenome (17,164 bp) encodes 37 genes, including 13 protein-coding genes (PCGs), 22 transfer RNA (tRNAs), two ribosomal RNA (rRNAs), and one control region (CR). Most of the genes were encoded on majority strand, except nad6 and eight tRNAs. The nonsynonymous/synonymous ratio in all PCGs indicates strong negative selection among all Tupaiidae species. The comparative study of CRs revealed the occurrence of tandem repeats (CGTACA) found in T. nicobarica. The phylogenetic analyses (ML and BA) showed distinct clustering of T. nicobarica with high branch supports and depict a substantial divergence time (11.4 to 18.8 MYR) from the ancestor lineage of Tupaiidae. The 16S rRNA dataset corroborates the taxonomic rank of two subspecies of T. nicobarica from the Great and Little Nicobar Islands. The present study suggests the assembly of whole-genome to improve the understanding of evolutionary relationships of treeshrews and its implication in biomedical research.
Anti-inflammatory Effects of Mesenchymal Stem Cells and their Secretomes
in Pneumonia
Authors: Li Zongjin , Hezam Kamal , Mo Rigen , Wang Chen , Liu Yue 
Published: September 2021

:
Mesenchymal stem cells (MSCs) are multipotent progenitor cells that play crucial roles in the microenvironment of injured tissues. The potential therapeutics of MSCs have attracted extensive attention for several diseases such as acute respiratory distress syndrome (ARDS) and novel coronavirus disease 2019 (COVID-19) pneumonia. MSC-extracellular vesicles have been isolated from MSC-conditioned media (MSC-CM) with similar functional effects as parent MSCs. The therapeutic role of MSCs can be achieved through the balance between the inflammatory and regenerative microenvironments. Clinical settings of MSCs and their extracellular vesicles remain promising for many diseases, such as ARDS and pneumonia. However, their clinical applications remain limited due to the cost of growing and storage facilities of MSCs with a lack of standardized MSC-CM. This review highlights the proposed role of MSCs in pulmonary diseases and discusses the recent advances of MSC application for pneumonia and other lung disorders.
Access, Use, and Patient-Reported Experiences of Emergency Care During the COVID-19 Pandemic: Population-Based Survey
Authors: Gray Jodi , Partington Andrew , Karnon Jonathan 
Published: September 2021

Background
An increase in the number of people presenting to emergency departments (EDs) is contributing to ED overcrowding. In the early stages of the COVID-19 pandemic, there was a significant reduction in the number of ED presentations in Australia, creating an opportunity to learn from patients’ experiences of alternative management options.

Objective
The aim of this study is to report on the use and experience of health services by Australian adults experiencing a health issue during the COVID-19 pandemic for which they would have presented at an ED prior to the pandemic.

Methods
An online survey was conducted in May 2020. Reported health issues were categorized using an existing classification system. Data collected included demographics, care pathways, levels of concern at times of health issue and survey completion, and patient-reported experiences with care.

Results
A total of 1289 eligible respondents completed the survey. Almost 25% (309/1289) of respondents avoided an ED presentation, of which 58% (179/309) used an alternative form of health care and 42% (130/309) self-managed. Respondents making face-to-face or telehealth appointments with their general practitioner (GP) reported high levels of ED avoidance (135/286, 47%) and mostly positive experiences of care provided by GPs. A high proportion of those who self-managed reported high levels of concern at the time of completing the survey (42/130, 32%).

Conclusions
Telehealth consultations with GPs may be a more promotable alternative to the ED beyond the COVID-19 pandemic, providing easier access to a doctor with access to patients’ medical histories than an appointment for a face-to-face consultation. GP telehealth consultations may also address barriers to accessing health care for those with potentially the greatest need. The reported use and positive experiences with GP telehealth appointments should inform further research on their appropriateness as an alternative to the ED.
Reducing Delays in Diagnosing Primary Immunodeficiency Through the Development and Implementation of a Clinical Decision Support Tool: Protocol for a Quality Improvement Project
Authors: Kumar Bharat , Zetumer Samuel , Swee Melissa , Endelman Ellen L Keyser , Suneja Manish , Davis Benjamin 
Published: September 2021

Background
Primary immunodeficiencies (PIs) are a set of heterogeneous chronic disorders characterized by immune dysfunction. They are diagnostically challenging because of their clinical heterogeneity, knowledge gaps among primary care physicians, and continuing shortages of clinically trained immunologists. As a result, patients with undiagnosed PIs are at increased risk for recurrent infections, cancers, and autoimmune diseases.

Objective
The aim of this research is to develop and implement a clinical decision support (CDS) tool for the identification of underlying PIs.

Methods
We will develop and implement a CDS tool for the identification of underlying PIs among patients who receive primary care through a health care provider at the University of Iowa Hospitals and Clinics. The CDS tool will function through an algorithm that is based on the Immune Deficiency Foundation’s 10 Warning Signs for Primary Immunodeficiency. Over the course of a year, we will use Lean Six Sigma principles and the Define, Measure, Analyze, Improve, and Control (DMAIC) framework to guide the project. The primary measure is the number of newly diagnosed PI patients per month. Secondary measures include the following: (1) the number of new patients identified by the CDS as being at high risk for PI, (2) the number of new PI cases in which immunoglobulin replacement or rotating antibiotics are started, (3) the cost of evaluation of each patient identified by the CDS tool as being at high risk for PIs, (4) the number of new consults not diagnosed with a PI, and (5) patient satisfaction with the process of referral to the Immunology Clinic.

Results
This study was determined to not be Human Subjects Research by the Institutional Review Board at the University of Iowa. Data collection will begin in August 2021.

Conclusions
The development and implementation of a CDS tool is a promising approach to identifying patients with underlying PI. This protocol assesses whether such an approach will be able to achieve its objective of reducing diagnostic delays. The disciplined approach, using Lean Six Sigma and the DMAIC framework, will guide implementation to maximize opportunities for a successful intervention that meets the study’s goals and objectives as well as to allow for replication and adaptation of these methods at other sites.

International Registered Report Identifier (IRRID)
PRR1-10.2196/32635
Infection in Patients with Suspected Thrombotic Microangiopathy Based on Clinical Presentation
Authors: Thoreau Benjamin , von Tokarski Florent , Bauvois Adeline , Bayer Guillaume , Barbet Christelle , Cloarec Sylvie , Mérieau Elodie , Lachot Sébastien , Garot Denis , Bernard Louis , Gyan Emmanuel , Perrotin Franck , Pouplard Claire , Maillot François , Gatault Philippe , Sautenet Bénédicte , Rusch Emmanuel , Frémeaux-Bacchi Véronique , Vigneau Cécile , Fakhouri Fadi , Halimi Jean-Michel 
Published: September 2021

Background and objectives
In contrast to shigatoxin-associated Escherichia coli (STEC) causing hemolytic uremic syndrome, STEC-unrelated infections associated with thrombotic microangiopathy are less characterized.

Design, setting, participants, & measurements
Our retrospective study in a four-hospital institution of 530 consecutive patients with adjudicated thrombotic microangiopathies during the 2009–2016 period studied STEC-unrelated infections’ epidemiology and major outcomes (death, acute dialysis, and major cardiovascular events).

Results
STEC-unrelated infection was present in 145 of 530 (27%) patients, thrombotic microangiopathies without infection were present in 350 of 530 (66%) patients, and STEC causing hemolytic and uremic syndrome was present in 35 of 530 (7%) patients. They (versus thrombotic microangiopathy without infection) were associated with age >60 years (36% versus 18%), men (53% versus 27%), altered consciousness (32% versus 11%), mean BP <65 mm Hg (21% versus 4%), lower hemoglobin and platelet count, and AKI (72% versus 49%). They were associated with more than one pathogen in 36 of 145 (25%) patients (either isolated [14%] or combined [86%] to other causes of thrombotic microangiopathy); however, no significant clinical or biologic differences were noted between the two groups. They were more frequently due to bacteria (enterobacteria [41%], Staphylococcus aureus [11%], and Streptococcus pneumonia [3%]) than viruses (Epstein–Barr [20%], cytomegalovirus [18%], influenza [3%], hepatitis C [1%], HIV [1%], and rotavirus [1%]). STEC-unrelated infections were independent risk factors for in-hospital death (odds ratio, 2.22; 95% confidence interval, 1.18 to 4.29), major cardiovascular event (odds ratio, 3.43; 95% confidence interval, 1.82 to 6.69), and acute dialysis (odds ratio, 3.48; 95% confidence interval, 1.78 to 7.03). Bacteria (versus other pathogens), and among bacteria, enterobacteria, presence of more than one bacteria, and E. coli without shigatoxin were risk factors for acute dialysis.

Conclusions
Infections are frequent thrombotic microangiopathy triggers or causes, and they are mostly unrelated to STEC. Infections convey a higher risk of death and major complications. The most frequent pathogens were enterobacteria, S. aureus, Epstein–Barr virus, and cytomegalovirus.

Podcast
This article contains a podcast at https://www.asn-online.org/media/podcast/CJASN/2021_09_07_CJN17511120.mp3
Pengetahuan Masyarakat dalam Melakukan Rekonstruksi Rumah Tinggal Pasca Gempa di Kabupaten Donggala
Authors: Yusriah A.R. , Fahirah Fahirah 
Published: September 2021

Gempa adalah suatu kejadian alam yang tidak bisa dihindari mengakibatkan banyaknya bangunan yang rusak, konstruksi bangunan memainkan peran penting dalam proses pembangunan disetiap daerah, dalam hal ini pengetahuan masyarakat saat membangun kembali rumah sangat penting untuk diketahui. Penelitian ini bertujuan mengindentifikasi pengetahuan masyarakat dalam melakukan rekonstruksi rumah tinggal pasca gempa. Metode yang digunakan dalam penelitian ini adalah dengan cara penyebaran kuesioner kepada masyarakat yang terdampak bencana khususnya di Desa Marana sebanyak 80 kepala keluarga. Analisis data menggunakan statistik deskriptif, uji reliabilitas, Relative Rank Index (RRI) dan korelasi Spearman’s Rho. Hasil penelitian menunjukkan bahwa variabel dominan masyarakat dalam melakukan rekonstruksi rumah tinggal adalah mengetahui cara memperbaiki tulangan pada dinding pemikul dengan nilai RRI sebesar 0,820, mengetahui cara memperbaiki tulangan pada kolom dengan nilai RRI sebesar 0,800, mengetahui jenis campuran untuk kolom dengan nilai RRI sebesar 0,780, mengetahui perbandingan campuran beton untuk kolom dengan nilai RRI sebesar 0,760, mengetahui tulangan yang baik digunakan untuk kolom dengan nilai RRI sebesar 0,740. Adapun hubungan antara variabel dominan tertinggi adalah perilaku masyarakat dalam mengetahui cara memperbaiki tulangan pada dinding pemikul berkorelasi dengan mengetahui cara memperbaiki tulangan pada kolom, memperoleh nilai tertinggi yaitu 0,672 termasuk kedalam kriteria korelasi kuat.
Performing Theatre and Democracy
Authors: Henriques Leila 
Published: September 2021

This collection of performances that is linked to this chapter was created as part of the MA exchange project between NTNU and DFL (Drama for Life). Students used performance ethnography as a method for generating performance material in answer to the challenge of building democracy through theatre. South Africa has a rich theatre history that has always engaged with the South African political narrative. Through developing an understanding of the many theatre-making processes that created this unique history, as well as through exploring other contemporary South African performances, students created and tracked their own research methodology so that they were able to hold up a mirror to the world around them. While each performance captured the individual perspective of the performer, they also engaged directly and indirectly with broader South African realities.
The course consisted of four components, each shaped by the individual’s journey into their own research methodology. These were: generating material, interpreting the material, rehearsing the material and performing the material. This submission consists of a framing statement written by the lecturer as well as a collection of ten performances that include a short framing statement from each performer. Permission was obtained from all the students to showcase their work apart from one student who has submitted it under a pseudonym. Out of this exploration and through a practical laboratory, students created an embodied experience that addressed the notion of democracy.
The value of the work was to gain a fresh embodied perspective of democracy in South Africa. It spoke to our unique South African theatre-making legacy, but also challenged and disrupted our understanding of what democracy is and how it might be performed.
The Iconicity of Russian Culture
Authors: Darenskiy Vitaliy Yu. 
Published: September 2021

Russian culture should be adequately represented in the modern cultural consciousness as a unique phenomenon. Among new approaches there seem to be three that can be considered the most important and promising. 1) The concept of the iconicity of Orthodox culture, created by V.V. Lepakhin, that is now being developed by a significant number of interesting authors in various aspects. 2) The concept of Russian literature Easterness (Paskhalnost), substantiated by I.A. Yesaulov and potentially applicable to the general specific characteristics of Russian culture as a whole. 3) The concept of Russian culture as the “culture of transformation”, in contrast to the Western culture of individual “self-realization”. The purpose of this article is to review the most important works within the outlined conceptual field and formulate general principles for understanding the iconicity of Russian culture as its ontological basis. From the methodological point of view, we are talking about a kind of “archeology of culture” (by analogy with the “archeology of knowledge” of M. Foucault) – that is, the discovery of the primary historical foundations of Russian culture, which were later obscured by the influence of Western culture, especially in its secular forms. In the West, the original and universal principle of the iconicity of Christian culture was gradually replaced by the principle of “sculpturality”. If iconicity is the focus of man and every creature on the Creator and on his highest heavenly perfection, which presupposes the path of transformation and the clear distinction between the created and non-created; then sculpturality is the self-sufficiency of man and all creations, closing them in their proud self-sufficiency, and thereby closing the way for their transformation. Since the Christian East has preserved Orthodoxy, it is here that the original “matrix” of Christian culture has been preserved, indestructible by any later Western influences, although it has experienced strong deformations and “pseudomorphoses” under their onslaught. Iconicity is the original ontology of culture as such: on the one hand, it preserves the original paradise connection with eternal and perfect existence at the moment of creation; on the other hand, it also carries the act of ontological catastrophe bringing death, evil and imperfection into the world. The concept of Russian culture “iconicity” is considered to be the most important theoretical achievement of modern Russian thought. It combines, on the one hand, cultural and theological accuracy, and, on the other, – the huge practical potential for the revival of the national spirit.
PENGOLAHAN DATA SISWA PADA SEKOLAH MENENGAH KEJURUAN 1 KARTIKATAMA KOTA METRO
Authors: Arthalita Ika , Hidayat Arif 
Published: September 2021

Penelitian ini merupakan hasil dari analisis dan observasi yang penulis lakukan terhadap sistem informasi yang sedang berjalan dan hasil perancangan yang baru pada pengolahan data siswa menggunakan Microsoft Excel hal ini mengakibatkan kesulitan dalam pengelolahan data siswa seperti kesulitan dalam proses pencarian dan pemisahan data siswa bila ada permintaan pada pembuatan laporan masih membingungkan dikarenakan banyaknya data siswa yang hilang diakibatkan oleh penyimpanan berkas yang belum tertata dengan baik. Tujuan penelitian ini adalah untuk mengetahui dan merancang pengelolahan data siswa, Untuk mempercepat pengolahan data dan pencarian data, untuk mempercepat dalam pembuatan laporan data siswa. Penelitian menggunakan metode pengolahan data berupa observasi, interview, dan dokumentasi. Sedangakan metode perancangan sisten informasi menggunakan Bagan Alir Dokumen, Data Flow Diagram, Entity Relationship Diagram. Sofware pendukung dalam pembuatan aplikasi pengolahan data siswa ini menggunakan Java, Database MySQL, Appserv dan Design dengan NetBeans IDE 8.1. Dengan adanya rancangan aplikasi pengelolahan data siswa diharapkan dapat membantu dalam pengelolahan data siswa seperti pencarian dan pembuatan laporan dengan cepat pada bagian tata usaha SMK Kartikatama Kota Metro.
Effect of Implicit Voice Theories on Employee Constructive Voice and Defensive Silence: A Study in Education and Health Sector
Authors: Şahin Safiye , Çankir Bilal , Arslan Bahar Serez 
Published: September 2021

Abstract

Background and Purpose: Implicit voice theories are the beliefs that are related to employee silence. This study aims to examine the implicit voice theories (don’t embarrass the boss in public, negative career consequences of voice) as predictors of employee defensive silence and employee constructive voice.

Methodology: This study is a cross-sectional and an analytical study. The sample of this study consisted of 494 participants working in the healthcare sector (n = 277) and education sector (n = 217) in Turkey. We performed linear regression analyses to test our hypotheses.

Results: We found that power distance and negative career consequences of voice predicted employee defensive silence while proactive personality and the belief of “don’t embarrass the boss in public” predicted employee constructive voice. These results confirm the effect of implicit voice theories on employee silence and voice.

Conclusions: Employees holding the belief of “don’t embarrass the boss in public” and “negative career consequences of voice” tend to remain silent or prefer to speak with their managers privately, which prevent sharing ideas in group interactions in organization. Therefore, managers must combat the belief that speaking up is risky. In order to change these beliefs in a positive way, they should make their subordinates feel that speaking up is a valuable behavior in organization.
A TAXONOMIC REVISION OF CYRTANDRA (GESNERIACEAE) IN SULAWESI, INDONESIA
Authors: Atkins H. J. , Kartonegoro A. 
Published: September 2021

Taxonomic evaluation of Cyrtandra (Gesneriaceae) from Sulawesi has resulted in the recognition of 39 species. Of these, four species are described as new here: Cyrtandra balgooyi H.J.Atkins & Karton., C. flavomaculata H.J.Atkins & Karton., C. longistamina H.J.Atkins & Karton. and C. parvicalyx H.J.Atkins & Karton. Eleven names are lectotypified and three names are placed in synonymy. Provisional conservation assessments suggest that seven of these species are Critically Endangered, six are Endangered, three are Vulnerable, two are Data Deficient and 21 are of Least Concern. Illustrations, distribution maps, and a key to the Cyrtandra species of Sulawesi are presented.
Evaluasi taksonomi Cyrtandra (Gesneriaceae) dari Sulawesi menghasilkan pengenalan 39 jenis. Diantara jenis-jenis tersebut, empat jenis dideskripsikan sebagai jenis yang baru disini: Cyrtandra balgooyi H.J.Atkins & Karton., C. flavomaculata H.J.Atkins & Karton., C. longistamina H.J.Atkins & Karton. and C. parvicalyx H.J.Atkins & Karton. Sebelas nama telah dilektotipifikasikan dan tiga nama ditempatkan dalam sinonim. Penilaian konservasi sementara menyarankan bahwa tujuh dari jenisjenis ini dinyatakan Critically Endangered, enam dinyatakan Endangered, tiga dinyatakan Vulnerable, dua dinyatakan Data Deficient dan 21 sisanya dinyatakan Least Concern. Ilustrasi, peta distribusi dan kunci identifikasi untuk jenis-jenis Cyrtandra dari Sulawesi disajikan.