bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Novel Ferroptosis-Related Gene Signature Robustly Predicts Clinical Prognosis And Tumor Immunity in Patients With Kidney Renal Clear Cell Carcinoma
Authors: Song Wei , Kang Weiting , Zhang Qi 
Published: April 2021

Abstract
Objective: This study aimed to construct a ferroptosis-related gene signature to predict clinical prognosis and tumor immunity in patients with kidney renal clear cell carcinoma (KIRC).Methods: The mRNA expression profiles and corresponding clinical data of KIRC patients were downloaded from The Cancer Genome Atlas (TCGA), which were randomly divided into training (398 patients) and validation set (132 patients). The iron death related (IDR) prediction model was constructed based on training set and 60 ferroptosis-related genes from previous literatures, followed by prognostic performance evaluation and verification using the validation set. Moreover, functional enrichment, immune cell infiltration, metagene clusters correlation, and TIDE scoring analyses were performed. Results: In total, 23 ferroptosis-related genes were significantly associated with overall survival (OS). The IDR prediction model (a 10-gene signature) was then constructed to stratify patients into two risk groups. The OS of KIRC patients with high-risk scores was significantly shorter than those with low-risk scores. Moreover, the risk score was confirmed as an independent prognostic predictor for OS. The positive and negative correlated genes with this model were significantly enriched in p53 signaling pathway, and cGMP-PKG signaling pathway. The patients in the high-risk group had higher ratios of plasma cells, T cells CD8, and T cells regulatory Tregs. Furthermore, IgG, HCK, LCK, and Interferson metagenes were significantly correlated with risk score. By TIDE score analysis, patients in the high-risk group could benefit from immunotherapy.Conclusions: The identified ferroptosis-related gene signature is significantly correlated with clinical prognosis and immune immunity in KIRC patients.
Circle RNA PLEC Acts as a Sponge of Microrna-198 to Promote Gastric&nbsp;Carcinoma Cell Resistance to Paclitaxel and Tumorigenesis
Authors: Zhou Ning , Wang Wei , Xu Chunlei , Yu Wenyan 
Published: April 2021

Abstract
Gastric carcinoma (GC) is one of the most frequent type of malignancy all over the world. The resistance of Paclitaxel (PTX) has become the obstacle of the prognosis of GC, and the underlying mechanism of is not clear. Previous study showed that GC-related circRNAs have been identified via microarray analysis and bioinformatics analysis, and we discovered that circPLEC (hsa_circ_0085923) remarkably upregulated in GC cells. The molecular mechanism of circPLEC in PTX-resistance GC cells still needs to explore.The expression of circPLEC in GC cells, PTX-resistant GC cells and GC tissues was analyzed by qRT-PCR. CircPLEC was knocked down in GC cells by transfecting shRNA, and then we used the CCK-8 assay, transwell, and FCM analysis to verify the effect in circPLEC in PTX-resistant GC cells. Additionally, we used luciferase reporter assays to confirm the relationship among circPLEC, miR-198 and MUC19.In present study, qRT-PCR exhibited that circPLEC were upregulated in PTX-resistant GC tissues and cells, indicating that circPLEC boost the PTX resistance of GC. circPLEC downregulation could weaken the GC resistance to PTX and the ability of tumorigenesis, migration and invasion, and promote the apoptosis of PTX-resistant GC cells. miR-198 inhibitor could revise the effect of circPLEC downregulation in PTX-resistant GC cells, and MUC19 downregulation could weaken the GC resistance to PTX and the tumorigenesis, and improve the apoptosis of PTX-resistant GC cells. In summary, circPLEC acts as a sponge of miR-198 to promote the PTX resistance and tumorigenesis of GC cells by regulating MUC19 expression.
A Nomogram to Predict Lymph Node Metastasis Risk for Early&nbsp;Esophageal Squamous Cell Carcinoma
Authors: Duan Xiaofeng , Shang Xiaobin , Yue Jie , Ma Zhao , Chen Chuangui , Tang Peng , Jiang Hongjing , Yu Zhentao 
Published: April 2021

Abstract
Background: A nomogram was developed to predict lymph node metastasis (LNM) for patients with early-stage esophageal squamous cell carcinoma (ESCC).Methods: We used the clinical data of ESCC patients with pathological T1 stage disease who underwent surgery from January 2011 to June 2018 to develop a nomogram model. Multivariable logistic regression was used to confirm the risk factors for variable selection. The risk of LNM was stratified based on the nomogram model. The nomogram was validated by an independent cohort which included early ESCC patients underwent esophagectomy between July 2018 and December 2019.Results: Of the 223 patients, 36 (16.1%) patients had LNM. The following three variables were confirmed as LNM risk factors and were included in the nomogram model: tumor differentiation (odds ratio [OR]=3.776, 95% confidence interval [CI] 1.515-9.360, p=0.004), depth of tumor invasion (OR=3.124, 95% CI 1.146-8.511, p=0.026), and tumor size (OR=2.420, 95% CI 1.070-5.473, p=0.034). The C-index was 0.810 (95% CI 0.742-0.895) in the derivation cohort (223 patients) and 0.830 (95% CI 0.763-0.902) in the validation cohort (80 patients). Conclusions: A validated nomogram can predict the risk of LNM via risk stratification. It could be used to assist in the decision-making process to determine which patients should undergo esophagectomy and for which patients with a low risk of LNM, curative endoscopic resection would be sufficient.
MKL1 Regulates Hepatocellular Carcinoma Cell Proliferation, Migration and Apoptosis via the COMPASS Complex and NF-Κb Signaling
Authors: Liu Zhao , Sun Jiuzheng , Li Chuanzhi , Xu Liyou , Liu Jun 
Published: April 2021

Abstract
Background

Histone modification plays essential roles in hepatocellular carcinoma (HCC) pathogenesis, but the regulatory mechanisms remain poorly understood. In this study, we aimed to analyze the roles of Megakaryoblastic leukemia 1 (MKL1) and its regulation of COMPASS (complex of proteins associated with Set1) in HCC cells.
Methods

MKL1 expression in clinical tissues and cell lines were detected by bioinformatics, qRT-PCR and western blot. MKL1 expression in HCC cells were silenced with siRNA, followed by cell proliferation evaluation via Edu staining and colony formation, migration and invasion using the Transwell system, and apoptosis by Hoechst staining. HCC cell tumorigenesis was assessed by cancer cell line-based xenograft model, combined with H&E staining and IHC assays.
Results

MKL1 expression was elevated in HCC cells and clinical tissues which was correlated with poor prognosis. MKL1 silencing significantly repressed proliferation, migration, invasion and colony formation but enhanced apoptosis in HepG2 and Huh-7 cells. MKL1 silencing also inhibited COMPASS components and p65 protein expression in HepG2 and Huh-7 cells. HepG2 cell tumorigenesis in nude mice was severely impaired by MKL1 knockdown, resulted into suppressed Ki67 expression and cell proliferation.
Conclusion

MKL1 promotes HCC pathogenesis by regulating hepatic cell proliferation, migration and apoptosis via the COMPASS complex and NF-κB signaling.
Valorization of dietary lemon pomace waste to enhance lucerne silage composition and quality characteristics, and ruminal biogas production and fermentation
Authors: Besharati Maghsoud , Palangi Valiollah , Nemati Zabihollah , Safari Rashid , Salem Abdelfattah Z. M. 
Published: April 2021

Abstract
The purpose of this study was to investigate the effect of adding various levels of waste sour lemon pomace to lucerne on the properties and ruminal gas production of silage. Levels of 0 (Control), 25 (L1), 50 (L2), 75 (L3), and 100 (L4) % lemon pomace were replaced by lucerne for silage preparation and silenced for 60 days. The experiment was conducted in a completely randomized design with three replications (3 silos per treatment). After opening the silos, pH and dry matter were measured immediately, and the dried samples were kept at -20 until further tests. The silage pH decreased with the addition of lemon pomace compared to the control (p < 0.05). Total silage volatile fatty acids and dry matter content increased with adding lemon pomace. The results of gas production also showed that lemon pomace increased the in vitro gas production volume. Adding lemon pomace to lucerne silage due to the high pectin content in these agricultural wastes caused a rapid decrease of silage pH and an acidic environment. It prevented the growth of non-beneficial bacterial species. The obtained data showed that waste sour lemon has a good potential to use as a livestock feedstuff that can be useful in reducing the cost of ruminant production and preventing environmental pollution.
Should we avoid heparin based anticoagulants in “COVID-19 infection-vaccination related thrombosis”? Insights from the HIT like syndrome observed with infected cases.&nbsp;
Authors: AbdelMassih Antoine , Hozaien Rafeef , Shershaby Meryam El , Kamel Aya , Ismail Habiba-Allah , Fouda Raghda 
Published: April 2021

Abstract
Background: Recent reports of the prothrombotic states announced in the recipients of the ChAdOx vaccine, similar to HIT sounded alarm bells internationally.The sole outcome parameter of this systematic review was to detect similar cases of Prothrombotic state in COVID-19 infected patientsMethodology: Embase, Medline and the Cochrane Central Register were used to search for specic keywords such as “COVID-19” OR “SARS-CoV-2” AND “Heparin induced thrombocytopenia” for relevant publications up to 1st of April 2021. The systematic review was performed using PRISMA protocol.Results: Nine cases reports/series have been identified including 18 cases, venous thrombosis was the commonest type of observed thrombosis accounting for 72 % of cases, the mean age of cases was 57±4.Conclusion: Similar episodes of HIT were detailed in several case reports of COVID patients. This suggests a common pathogenesis and warrants a screening of all COVID-19 patients presenting with thrombo-embolism using anti-PF4 antibodies and using non heparin based anticoagulants for their management.
A Review of Thromboembolic Events in Hospitalized COVID-19 Patients
Authors: Fontelo Paul , Bastola Mrigendra M , Zheng Zhaonian , Baik Seo Hyon 
Published: April 2021

Abstract
Context: A higher incidence of thromboembolic disorders in COVID-19 has been reported by many clinicians worldwide.Objective, Design and Data Sources: Selected studies found in PubMed that reported thromboembolic events were included for meta-analysis using weighted fixed and random effects. Data from 19 articles on cohort studies in patients diagnosed with COVID-19 and thromboembolic events, including thrombosis and embolism were included in this review.Results: The likelihood for developing thromboembolic disorders in hospitalized COVID-19 patients was 0.28 (95% CI 0.21–0.36).Conclusion: This study further validates the increased risk of VTE in COVID-19 patients when compared to healthy, non-hospitalized people, and hospitalized patients. These findings will be useful to researchers and medical practitioners caring for COVID-19 patients.
Water quality of the Gualaxo do Norte and Carmo rivers after the Fundão dam collapse, Mariana, MG
Authors: Santana Felipe Carvalho , Francelino Márcio R. , Schaefer Carlos E. G. R. , Veloso Gustavo V. , Fernandes-Filho Elpídio Inácio , Santana Adalgisa J. P. , Timo Lucas , Rosa André 
Published: April 2021

Abstract
We investigated the effects of Fe-mining tailings of the Fundão dam on water quality, concerning physical and chemical parameters, aiming to infer the recovery process of the affected areas, and prospects of water quality in the coming years. Water quality data from the Gualaxo do Norte and Carmo rivers were evaluated in three moments: (i) before the dam rupture (1999–2000); (ii) one year after the rupture of the Fundão dam (2016); and (iii) after the implementation of recover mitigation activities (2017–2019). Concerning the variables evaluated, the mean concentrations of Fe and Mn were higher in the dry period, and increasing turbidity was detected in the rainy season. The turbidity, even after the recovery measures, peaked at 300 and 400 NTU in 2017 and 2019, respectively. The concentrations of dissolved Fe, Mn and Cr, closely related to the tailings, decreased with rainfall by a dilution mechanism. Conversely, the concentrations of Cd, Al, Zn, and Cu increased in the rainy season, probably by greater erosion. The implementation of the recovery actions at the Gualaxo do Norte headwaters are helping to reduce the Mn and Fe concentrations and turbidity downriver, all associated with the Fe-mining tailings.
Healthcare Waste Generation, Composition and management Practice in Dilla University Referral Hospital: A cross-sectional study
Authors: Kanno Girum Gebremeskel , Gondol Belay Negassa , Mamo Tizalegne Tesfaye ,   Abriham Shiferaw Areba1, Lagiso Zemachu Ashuro , Tsebe Awash Alembo , Legesse Miheret Tesfu , Dansa Daniel Abera , Admasu Mulugeta , Tesfaye Dagmawit , Sisay Gizaw , Eshete Negassa , Aregu Mekonnen Birhanie 
Published: April 2021

Abstract

Background: Improper collection, segregation, transportation, treatment and disposal of healthcare wastes waste pose risk to public health and the environment. The aim of this assessment was to determine the healthcare waste generation rate, composition and management practice in Dilla University Referral Hospital.Methods: An institutional based cross-sectional study was conducted in Dilla University Referral hospital from May to June 2019. The quantitative data was collected for eight consecutive days using direct measurment of healthcare waste. The qualitative data was collected by using questionnaire, observation checklist, and in-depth interview. The qualitative data was collected and transcribed and thematic content analysis was done.Result: A total of 1212.5 kg of healthcare waste was generated in the study period with an average of 151.56 kg (±73.4). The hazardous fraction of the healthcare waste was 581.9 kg (48 %) while the generation rate was 0.866 kg bed-1day-1. From the total healthcare waste, general waste was the highest 630.6 kg (52%) followed by infectious waste which was 299.5 kg (24.7%). There was no segregation of healthcare waste by type at the point of generation and there was no pre-treatment of infectious wastes. Placenta pit was used for pathological wastes and open burning was the main disposal mechanism for the remaining wastes.Conclusion: The finding indicated that the proportion of hazardous waste generated from the hospital was above the threshold set by the World Health Organization. There was a lack of appropriate waste segregation, storage, transport, treatment and disposal practices in the referal hospital.
Study of blood levels of Lactate, Vitamin D and Nucleated Red Blood Cells for early prediction of Hypoxic–Ischemic Encephalopathy: A case-control study
Authors: Saad Ahmed Hosni , Mahmoud Ahmed Thabet , Soliman Mohamed Abdelrehem , Abdelaziz Alyaa Ahdy 
Published: April 2021

Abstract
Objective and background: Perinatal asphyxia is a serious medical condition in which there is lack of oxygen or blood flow during, or even after the labor process. So, this study aimed to study serum blood levels of Lactate, vitamin D and count of nucleated Red Blood Cells (nRBCs) per 100 White Blood Cells (WBCs) as early biological predictors of Hypoxic–Ischemic Encephalopathy (HIE) after birth Asphyxia.Methods: A retrospective case-control study was conducted on 20 neonates with HIE comprising the cases (APGAR ≤ 5) and another 20 neonates representing the control group with their age, sex, and weight matched, admitted to NICU from March 2020 to the end of September 2020 at Menoufia University Hospital and El-Bagour Central Hospital, Egypt. Venous blood samples for lactate, vitamin D and nucleated RBCs were drawn during 1st 6h of birth and sent for analysis. Sarnat score was used to assess the severity of HIE.Results: Venous blood levels of lactate and nucleated Red Blood Cells per 100 White Blood Cells (nRBC/100 WBC) counts were significantly higher in HIE neonates than in the control group with a highly significant difference (P-value < 0.001). While serum Vitamin D was significantly lower in the HIE group than in the control (P-value < 0.001).Conclusion: Serum lactate level, vitamin D level and nRBCs/100 WBCs might be used as biomarkers in the early prediction of HIE.
Serotipos vacunales y no vacunales de Streptococcus pneumoniae en niños de Latinoamérica: revisión del último reporte SIREVA II
Authors: Sosa Delgado Nallely , Martinez Rojas Dianny , Lugo Judith 
Published: April 2021

Las infecciones por Streptococcus pneumoniae se encuentran dentro de las principales causas de muerte por enfermedades inmunoprevenibles en los niños menores de cinco años. Actualmente, existen vacunas con jugadas contra diversos serotipos de S. pneumoniae, por lo que su introducción ha sido una buena estrategia para la inmunización de la población en riesgo, además de contribuir indirectamente con la disminución de la resistencia antimicrobiana. Se realizó una revisión del último reporte disponible de los serotipos de S. pneumoniae circulantes en niños menores de cinco años residentes en Latinoamérica. Los serotipos vacunales 19A, 3, 14, 1 y no vacunal 12F, fueron los más frecuentes. En Latinoamérica la implementación de la vacuna PCV13 sigue siendo una excelente opción; sin embargo, con la introducción de las vacunas conjugadas se ha visto el fenómeno de enfermedad de reemplazo evidenciado con la circulación de diversidad de serotipos no vacunales. Es necesario realizar regularmente estudios epidemiológicos en los países latinoamericanos a fin de monitorear las estrategias de vacunación y el efecto de las vacunas conjugadas en la región.
Desprendimiento seroso de retina agudo inducido por cirugía en posición prono
Authors: Sardi Correa María Carolina , Cardona Bernal Susana , Ortiz Vásquez Juan José 
Published: April 2021

La posición en decúbito prono es utilizada en cirugía para acceder a diferentes estructuras anatómicas del paciente. Esta posición puede asociarse a pérdida visual postoperatoria en el 0,05 a 1 % de las complicaciones reportadas. El desprendimiento seroso de retina es la separación de la retina neurosensorial del epitelio pigmentario de la retina por acumulación de líquido, sin daño estructural, como resultado de cambios en la permeabilidad y perfusión de la coroides. Se presenta el caso de una mujer con indicación de extracción de prótesis glútea bilateral mediante cirugía en decúbito prono. Se identificó un desplazamiento del cojín posicionado en la cara y la cabecera no correspondía con la estructura ósea de la paciente. En sala de recuperación la paciente refirió pérdida visual en ojo izquierdo, por lo cual fue evaluada inmediatamente por Oftalmología encontrando una presión intraocular bilateral normal; un examen de fondo de ojo izquierdo evidenció múltiples áreas de desprendimiento de la retina. La tomografía con coherencia óptica confirmó la presencia de fluido subretiniano. Fue tratada con nepafenaco y prednisolona tópicas y evaluada a las 72 horas y a la semana, con resolución total del cuadro y agudeza visual corregida de 20/20. No existen reportes en la literatura de desprendimiento seroso de retina posterior a cirugía en prono. Al evaluar el caso se asumió que la incorrecta posición del cojín y el tamaño inadecuado de la cabecera fueron factores que podrían haber contribuido a estasis venoso del flujo coroideo aumentando súbitamente la presión hidrostática en la coroides, lo que provocó el desprendimiento seroso de retina.
Barriers to the Use of Clinical Decision Support for the Evaluation of Pulmonary Embolism: Qualitative Interview Study
Authors: Richardson Safiya , Dauber-Decker Katherine L , McGinn Thomas , Barnaby Douglas P , Cattamanchi Adithya , Pekmezaris Renee 
Published: April 2021

Background
Clinicians often disregard potentially beneficial clinical decision support (CDS).

Objective
In this study, we sought to explore the psychological and behavioral barriers to the use of a CDS tool.

Methods
We conducted a qualitative study involving emergency medicine physicians and physician assistants. A semistructured interview guide was created based on the Capability, Opportunity, and Motivation-Behavior model. Interviews focused on the barriers to the use of a CDS tool built based on Wells’ criteria for pulmonary embolism to assist clinicians in establishing pretest probability of pulmonary embolism before imaging.

Results
Interviews were conducted with 12 clinicians. Six barriers were identified, including (1) Bayesian reasoning, (2) fear of missing a pulmonary embolism, (3) time pressure or cognitive load, (4) gestalt includes Wells’ criteria, (5) missed risk factors, and (6) social pressure.

Conclusions
Clinicians highlighted several important psychological and behavioral barriers to CDS use. Addressing these barriers will be paramount in developing CDS that can meet its potential to transform clinical care.
Development of a Mobile App for Ecological Momentary Assessment of Circadian Data: Design Considerations and Usability Testing
Authors: Woolf Thomas B , Goheer Attia , Holzhauer Katherine , Martinez Jonathan , Coughlin Janelle W , Martin Lindsay , Zhao Di , Song Shanshan , Ahmad Yanif , Sokolinskyi Kostiantyn , Remayeva Tetyana , Clark Jeanne M , Bennett Wendy , Lehmann Harold 
Published: April 2021

Background
Collecting data on daily habits across a population of individuals is challenging. Mobile-based circadian ecological momentary assessment (cEMA) is a powerful frame for observing the impact of daily living on long-term health.

Objective
In this paper, we (1) describe the design, testing, and rationale for specifications of a mobile-based cEMA app to collect timing of eating and sleeping data and (2) compare cEMA and survey data collected as part of a 6-month observational cohort study. The ultimate goal of this paper is to summarize our experience and lessons learned with the Daily24 mobile app and to highlight the pros and cons of this data collection modality.

Methods
Design specifications for the Daily24 app were drafted by the study team based on the research questions and target audience for the cohort study. The associated backend was optimized to provide real-time data to the study team for participant monitoring and engagement. An external 8-member advisory board was consulted throughout the development process, and additional test users recruited as part of a qualitative study provided feedback through in-depth interviews.

Results
After ≥4 days of at-home use, 37 qualitative study participants provided feedback on the app. The app generally received positive feedback from test users for being fast and easy to use. Test users identified several bugs and areas where modifications were necessary to in-app text and instructions and also provided feedback on the engagement strategy. Data collected through the mobile app captured more variability in eating windows than data collected through a one-time survey, though at a significant cost.

Conclusions
Researchers should consider the potential uses of a mobile app beyond the initial data collection when deciding whether the time and monetary expenditure are advisable for their situation and goals.