bluuebunny/crossref_metadata_2025_split huggingface dataset:
Application of Bayesian Super-Resolution to Spectroscopic Data for Precise Characterization of Spectral Peak Shape
Authors: Tsujimori Kota , Hirotani Jun , Harada Shunta 
Published: October 2021

Abstract
The number of data points of digitally recorded spectra have been limited by the number of multi-channel detectors employed, which sometimes inhibits the precise characterization of spectral peak shape. Here we describe a methodology to increase the number of data points as well as the signal-to-noise (S/N) ratio by applying Bayesian super-resolution in the analysis of spectroscopic data. In our present method, first the hyperparameters for the Bayesian super-resolution are determined by a virtual experiment imitating actual experimental data, and the precision of the super-resolution reconstruction is confirmed by the calculation of errors from the ideal values. For validation of the super-resolution reconstruction of spectroscopic data, we applied this method to the analysis of Raman spectra. From 200 Raman spectra of a reference Si substrate with a data interval of about 0.8 cm-1, super-resolution reconstruction with a data interval of 0.01 cm-1 was successfully achieved with the promised precision. From the super-resolution spectrum, the Raman scattering peak of the reference Si substrate was estimated as 520.55 (+0.12, -0.09) cm-1, which is comparable to the precisely determined value reported in previous works. The present methodology can be applied to various kinds of spectroscopic analysis, leading to increased precision in the analysis of spectroscopic data and the ability to detect slight differences in spectral peak positions and shapes.
The confidante method to measure abortion: implementing a standardized comparative analysis approach across seven contexts.
Authors: Owolabi Onikepe Oluwadamilola , Giorgio Margaret , Leong Ellie , Sully Elizabeth 
Published: October 2021

Abstract
Background: Obtaining representative abortion incidence estimates is challenging in restrictive contexts. While the confidante method has been increasingly used to collect this data in such settings, there are several biases commonly associated with this method. Further, there are significant variations in how researchers have implemented the method and assessed/adjusted for potential biases, limiting the comparability and interpretation of existing estimates. This study presents a standardized approach to analyzing confidante method data, generates comparable abortion incidence estimates from previously published studies and recommends standards for reporting bias assessments and adjustments for future confidante method studies.Methods: We used data from previous applications of the confidante method in Côte d’Ivoire, Ethiopia, Ghana, Java (Indonesia), Nigeria, Uganda, and Rajasthan (India). We estimated one-year induced abortion incidence rates for confidantes in each context, attempting to adjust for selection, reporting and transmission bias in a standardized manner.Findings: In each setting, majority of the foundational confidante method assumptions were violated. Adjusting for transmission bias using self-reported abortions consistently yielded the highest incidence estimates compared with other published approaches. Differences in analytic decisions and bias assessments resulted in the incidence estimates from our standardized analysis varying widely from originally published rates.Interpretation: We recommend that future studies clearly state which biases were assessed, if associated assumptions were violated, and how violations were adjusted for. This will improve the utility of confidante method estimates for national-level decision making and as inputs for global or regional model-based estimates of abortion.Funding: UK Aid from the UK Government
A Categorization of Power Measurement and Power Consumption Models in the Telco Cloud
Authors: Depasquale Etienne-Victor , Salam Humaira Abdul , Davoli Franco 
Published: October 2021

Abstract
This article surveys the literature, over the period 2010-2020, on measurement of power consumption and relevant power models of virtual entities as they apply to the telco cloud. Hardware power meters are incapable of measuring power consumption of individual virtual entities co-hosted on a physical machine. Thus, software power meters are inevitable, yet their development is difficult. Indeed, there is no direct approach to measurement and, therefore, modeling through proxies of power consumption must be used. In this survey, we present trends, fallacies and pitfalls. Notably, we identify limitations of the widely-used linear models and the progression towards Artificial Intelligence / Machine Learning techniques as a means of dealing with the seven major dimensions of variability: workload type; computer virtualization agents; system architecture and resources; concurrent, co-hosted virtualized entities; approaches towards attribution of power consumption to virtual entities; frequency; and temperature.
Immunohistochemistry Study of Tumor Vascular Normalization and Anti-angiogenic Effects of Sunitinib Versus Bevacizumab Prior to Dose Dense Doxorubicin/cyclophosphamide Chemotherapy in HER2 Negative Breast Cancer
Authors: Yadav Kritika , Lim Joline , Choo Joan , Ow Samuel Guan Wei , Wong Andrea , Lee Matilda , Chan Ching Wan , Hartman Mikael , Lim Siew Eng , Ngoi Natalie , Tang Siau Wei , Ang Yvonne , Chan Gloria , Chong Wan Qin , Tan Hon Lyn , Tan Sing Huang , Goh Boon Cher , Lee Soo-Chin 
Published: October 2021

Abstract
PurposeTumor angiogenesis controlled predominantly by vascular endothelial growth factor and its receptor (VEGF-VEGFR) interaction plays a key role in the growth and propagation of cancer cells. However, the newly formed network of blood vessels is disorganized and leaky. Pre-treatment with anti-angiogenic agents can “normalize” the tumor vasculature allowing effective intra-tumoral delivery of standard chemotherapy.Immunohistochemistry (IHC) analysis was applied to investigate and compare the vascular normalization and anti-angiogenic effects of two commonly used anti-angiogenic agents, Sunitinib and Bevacizumab, administered prior to chemotherapy in HER2 negative breast cancer patients.MethodsThis prospective clinical trial enrolled 38 patients into a sunitinib cohort and 24 into a bevacizumab cohort. All received 4 cycles of doxorubicin/cyclophosphamide chemotherapy and pre-treatment with either sunitinib or bevacizumab. Tumor biopsies were obtained at baseline, after cycle 1 (C1) and cycle 4 (C4) of chemotherapy. IHC was performed to assess the tumor vascular normalization index (VNI), lymphatic vessel density (LVD), Ki67 proliferation index and expression of tumor VEGFR2. ResultsIn comparison to Bevacizumab, Sunitinib led to a significant increase in VNI post C1 and C4 (p <0.001 and 0.001) along with decrease in LVD post C1 (p= 0.017). Both drugs when combined with chemotherapy resulted in significant decline in tumor proliferation after C1 and C4 (baseline vs post C4 Ki67 index p=0.006 for Sunitinib vs p=0.021 for Bevacizumab). Bevacizumab resulted in a significant decrease in VEGFR2 expression post C1 (p=0.004). ConclusionSunitinib, in comparison to Bevacizumab showed a greater effect on tumor vessel modulation and lymphangiogenesis suggesting that its administration prior to chemotherapy might result in improved drug delivery.Clinical trial registrationClinicalTrials.gov: NCT02790580 (first posted June 6, 2016).
Wave-shaped microfluidic chip assisted point-of-care testing for accurate and rapid diagnosis of infections
Authors: Yin Binfeng , Wan Xinhua , Yang Mingzhu , Qian Changcheng , Sohan A S M Muhtasim Fuad 
Published: October 2021

Abstract
Background: Simultaneous and timely detection of C-reactive protein (CRP), procalcitonin (PCT), and interleukin-6 (IL-6) provides effective information for the accurate diagnosis of infections. Early diagnosis and classification of infections increase the cure rate while decreasing complications, which is significant for severe infections, especially for war surgery. However, traditional methods rely on laborious operations and bulky devices. On the other hand, point-of-care (POC) methods suffer from limited robustness and accuracy. Therefore, it is of urgent demand to develop POC devices for rapid and accurate diagnosis of infections to fulfill on-site militarized requirements.Methods: We developed a wave-shaped microfluidic chip (WMC) assisted multiplexed detection platform (WMC-MDP). WMC-MDP reduces detection time and improves repeatability through premixing of the samples and reaction of the reagents. We further combined the detection platform with the streptavidin-biotin (SA-B) amplified system to enhance the sensitivity while using chemiluminescence (CL) intensity as signal readout. We realized simultaneous detection of CRP, PCT, and IL-6 on the detection platform and evaluated the sensitivity, linear range, selectivity, and repeatability. Finally, we finished detecting 15 samples from volunteers and compared the results with commercial ELISA kits.Results: Detection of CRP, PCT, and IL-6 exhibited good linear relationships between CL intensities and concentrations in the range of 1.25-40 μg/mL, 0.4-12.8 ng/mL, and 50-1600 pg/mL. The limit of detection (LOD) of CRP, PCT, and IL-6 were 0.54 μg/mL, 0.11 ng/mL, and 16.25 pg/mL, respectively. WMC-MDP is capable of good adequate selectivity and repeatability. The whole detection procedure takes only 22 minutes that meets the requirements of a POC device. Results of 15 samples from volunteers were consistent with the results detected by commercial ELISA kits.Conclusion: WMC-MDP allows simultaneous, rapid, and sensitive detection of CRP, PCT, and IL-6 with satisfactory selectivity and repeatability, requiring minimal manipulation. However, WMC-MDP takes advantage of being a microfluidic device showing the coefficients of variation less than 10% enabling WMC-MDP to be a type of POCT. Therefore, WMC-MDP provides a promising alternative to point-of-care testing (POCT) of multiple biomarkers. We believe the practical application of WMC-MDP in militarized fields will revolutionize infection diagnosis for soldiers.
Differential V2-directed Antibody Responses in Non-human Primates Infected with SHIVs or Immunized with Diverse HIV Vaccines
Authors: Zolla-Pazner Susan , Weiss Svenja , Itri Vincenza , Pan Ruimin , Jiang Xunqing , Luo Christina , Morris Lynn , Malherbe Delphine , Barnette Philip , Alexander Jeff , Kong Xiang-Peng , Haigwood Nancy , Hessell Ann , Duerr Ralf 
Published: October 2021

Abstract
V2p and V2i antibodies (Abs) that are specific for epitopes in the V1V2 region of the HIV gp120 envelope (Env) do not effectively neutralize HIV but mediate Fc-dependent anti-viral activities that have been correlated with protection from, or control of HIV, SIV and SHIV infection. Here, we describe a novel molecular toolbox that allows the discrimination of antigenically and functionally distinct polyclonal V2 Ab responses. We identified different patterns of V2 Ab induction by SHIV infection and three separate vaccine regimens that will aid in fine tuning an optimized immunization protocol for inducing V2p and V2i Abs. We observed no, or weak and sporadic V2p and V2i Abs in non-vaccinated Tier 1 and Tier 2 SHIV-infected NHPs, but in contrast, strong V2p and/or V2i Ab responses after immunization with a V2-targeting vaccine protocol using a prime/boost regimen with gp120 DNA and a V1V2-scaffold protein. The V2-targeting vaccine protocol is superior to both natural infection and to immunization with whole Env constructs for inducing functional V2p- and V2i-specific responses. Strikingly, levels of V2-directed Abs correlated inversely with Abs specific for gp120 and peptides of V3 and C5. These data demonstrate that a V1V2-targeting vaccine have advantages over the imprecise targeting of SIV/SHIV infections and of whole Env-based immunization regimens for inducing a more focused functional V2p- and V2i-specific Ab response.
RNA-Seq Based Transcriptome Analysis of Ethanol Extract of Saffron Protective Effect Against Corticosterone-Induced PC12 Cell Injury
Authors: Chen Xi , Yang Ting , Zhang Cong' en , Ma Zhijie 
Published: October 2021

Abstract
Background
Saffron is a traditional Chinese herbal medicine, which is typically used in clinical to regulate anxiety, tension, and other depression-related conditions. The study aimed to explore the neuroprotective effect of ethanol extract of saffron (EES) on corticosterone (CORT)-induced injury in PC12 cells and further explored its potential mechanism.
Methods
The authenticity of saffron and the active components of EES were identified by a water test and ultra-performance liquid chromatography-time of flight mass spectrometry system. The screening of cytotoxicity for PC12 cells was incubated with EES in different concentrations for 24 h, and the protective efficacy of EES on CORT (500 µM) induced PC12 cell injury, cell viability was assessed by Cell Counting Kit-8 (CCK-8) assay. The DEGs of EES-protected PC12 cells were analyzed using the RNA-seq method, and the results were analyzed for GO and KEGG enrichment. The results of RNA-seq were verified by qPCR analysis.
Results
The saffron was initially identified as authentic in the water test and 10 compounds were identified by UPLC-MS. The results of CCK-8 demonstrated that EES at concentrations above 640 µg/mL exerted a certain cytotoxic effect, and PC12 cells pretreated with EES (20, 40, and 80 µg/mL) significantly reversed the 500 µM CORT-induced cell death. RNA-seq analysis showed that EES regulated 246 differential genes, which were mainly enriched in the MAPK signaling pathway. Dusp5, Dusp6, Gadd45b, Gadd45G, and Pdgfc were further validated by qPCR. Experimental data showed that the results of qPCR were consistent with RNA-seq.
Conclusions
These findings provide an innovative understanding of the molecular mechanism of the protective effect of EES on PC12 cells at the molecular transcription level, and the above molecules may be potential novel targets for antidepressant treatment.
Effects of Intercropping Patterns on Cadmium Tolerance of Perilla Frutescens and Soybean
Authors: han bolun , Zhang Wan , Fan Rongrong , Jing Rui , Lin Ruiyu , He Haibin , Zheng Xinyu 
Published: October 2021

Abstract
In this study, we intercropped hyperaccumulator Perilla frutescens with soybean to explore the effects of intercropping under cadmium stress based on the physiological growth indexes of plant, cadmium absorption capacity, and soil cadmium forms Experiments showed that compared with mono-cropping, intercropping soybean’s biomass, catalase (CAT) activity, peroxidase (POD) activity, total chlorophyll and net photosynthetic rate were significantly increased by 1042%~10658%, 1578%~7381%, 1345%~2337%, 376%~1500% and 7121%~31278%, while P frutescens’s significantly decreased by 9387%~9507%, 1562%~2572%, 12056%~15331%, 715%~756% and 3056%~5076% Under 5 and 10 mg kg-1 Cd treatments, the Cd content in roots, stems, leaves, pods and grains of intercropping soybean decreased significantly by 1072%~3174%, 1488%~2168%, 1995%~3413%, 2222%~5270% and 791%~1786% along with enrichment coefficient decreasing by 1486%~2787%, while those of P frutescens significantly increased by 8700%~2141%, 948%~2155%, 2305%~3766% and 1818%~9412% along with enrichment coefficient significantly increasing by 2000%~7775% Additionally, citric acid, succinic acid, oxalic acid and tartaric acid worked importantly in heavy metal detoxification in plants Strikingly, the content of soil exchangeable Cd (EXC) under intercropping was the highest, even higher than that of mono-cropping soybean and P frutescens, which accounted for 37%~42% under the same stress condition Therefore, intercropping Perilla frutescens with soybean provide a potential strategy for Cd phytoremediation
Bovine Tuberculosis in Colombia; Findings from Histopathological, Microbiological and Molecular Tests
Authors: Cadavid Paula Palomino , Balvin Dubel Ignacio , Buitrago Jhon Ruiz , Julio Rafael Villarreal , Ramos Enderson Murillo , Gutierrez Jesus Berdugo , García Rene Ramírez 
Published: October 2021

Abstract
Tuberculosis is a zoonotic infectious disease, caused by bacteria of the tuberculosis complex. Mycobacterium bovis causes tuberculosis in water buffaloes (Bubalus bubalis), and it can also infect other domestic animals, wild fauna, and humans. The diagnosis of bovine tuberculosis (bTB) through intradermal injections is challenging, and to understand the behaviour of other diagnostic tests is crucial. The aim of this research is to analyse three diagnostic tests against bTB in water buffaloes with positive test DPP. Different diagnostic tests were tested in 50 buffaloes diagnosed with bTB Cervical Comparative Test, from the Colombian lower tropic. Lesions compatible with bTB in 26 buffaloes with a positive DPP ; Four out of 2 samples of Mycobacterium bovis in DPP-positive buffaloes were isolated and confirmed positively in tissues using PCR-HRM, three buffaloes showed granulomatous lesions in histological analyses with positive microbiological isolation; 17 DPP-positive buffaloes had a positive PCR-HRM test and nine of these buffaloes showed no histological findings compatible with bTB, leading to purely molecular diagnosis. Evidence in histological, microbiological, and molecular findings in DPP is positive for the water buffaloes. None of the complementary tests showed 100% concordance with the intradermal results obtained with the Cervical Comparative Test for bTB.
Regional Water Resources Security Grading Evaluation Considering Both Visible and Virtual Water: A Case Study on Hubei Province, China
Authors: Du Yubo , Tu Yan , Li Zongmin , Nie Ling 
Published: October 2021

Abstract
The security of water resources is of great importance to long-term sustainability. In order to better ensure the security of water resources, a significant link is to conduct water resources security evaluation, which should be considered from many perspectives as it involves natural reserves, social production, the efficiency of use, and environmental protection. In this paper, a fuzzy Analytic Hierarchy Process Sort (AHPSort) II-entropy weight (EW) method for regional water resources security evaluation is proposed based on the security of visible water and virtual water. Firstly, this paper takes into account the criterion of efficiency of water use in addition to two other criteria of quantity of water resources, pressure on water resources to establish a comprehensive water resources security evaluation system. Secondly, a combination method of hesitant fuzzy language judgment and entropy weight is employed to obtain the weight of each indicator. Thirdly, AHPSort II is used to classify the security levels of the evaluated regions, in which the security levels of regional water resources are divided into five levels. Furthermore, a case study on the cities of Hubei Province, China is conducted to show the applicability of the proposed method, the effectiveness, and reliability of the method are then verified by being compared with a subjective method and an objective method as well as sensitivity analysis. Finally, according to the comprehensive evaluation results, specific management suggestions for improving the water resources security in the case are put forward.
Quantitative Values from Synthetic MRI Correlate with Breast Cancer Subtypes
Authors: Kazama Toshiki , Takahara Taro , Kwee Thomas C. , Nakamura Noriko , Kumaki Nobue , Niikura Naoki , Niwa Tetsu , Hashimoto Jun 
Published: October 2021

Abstract
Purpose To correlate quantitative T1, T2, and proton density (PD) values from synthetic MRI with breast cancer subtypes. Methods Twenty-eight breast cancer patients underwent MRI of the breast including synthetic MRI. T1, T2, and PD values were correlated with Ki-67. T1, T2, and PD values were compared between estrogen receptor (ER) positive and ER negative cancers, and between Luminal A and Luminal B cancers. The effectiveness of T1, T2, and PD in differentiating the ER-negative from the ER-positive group and Luminal A from Luminal B cancers was evaluated using receiver operating characteristic analysis.Results Mean T2 relaxation of ER-negative cancers was significantly higher than that of ER-positive cancers (p < .05). The T1, T2, and PD values exhibited a strong positive correlation with Ki-67 (Pearson's r = 0.75, 0.69, and 0.60 respectively; p < .001). Among ER-positive cancers (n=23), T1, T2, and PD values of Luminal A cancers were significantly lower than those of Luminal B cancers (p < .05). The areas under the curve (AUCs) of T1, T2, and PD for discriminating ER-negative from ER-positive cancers were 0.74 (95% confidence interval (CI): 0.54-0.88), 0.87 (95% CI: 0.69-0.97), and 0.62 (95% CI: 0.42-0.79), respectively. The AUCs of T1, T2, and PD values for discriminating Luminal A from Luminal B cancers were 0.83 (95% CI: 0.61-0.95), 0.75 (95% CI: 0.52-0.90), and 0.75 (95% CI: 0.53-0.91), respectively.Conclusion Quantitative values from synthetic MRI significantly correlate with subtypes of invasive breast cancers and may classify subtypes with reasonably good accuracy.
Curative Effect of Oblique Lateral Interbody Fusion in the Treatment of Lumbar Tuberculosis
Authors: Zhang Wenhui , Sun Weiqiang , Zhang Yuanyuan , Yang Fengguang , Yang Yuping , lu Mingdong , Guo Shifang , He Zongru , Liu Jie 
Published: October 2021

Abstract
Objective: This research aimed to investigate the curative effect of oblique lateral interbody fusion (OLIF) in the treatment of lumbar tuberculosis. Methods: A total of 32 patients, including 18 males and 14 females (29-58 years old, with the mean age of 47) who were admitted to our hospital from October 2016 to June 2020 and treated with oblique lateral interbody fusion combined with vertebral screw internal fixation, were retrospectively collected. The segmental distribution of lesions was L2- L316 cases, L3-L411 cases and L4-L55 cases. At first, preoperative antituberculous drug treatment was standardized for 2-4 weeks. Furthermore, intraoperative blood loss, operation time, postoperative complications, ESR, CRP, VAS, JOA and ASIA scores were recorded before and after surgery and at the last follow-up. In addition, antituberculous drug therapy was regulated continuously after surgery.Results: All patients were followed up for 12-17 months, with an average of 14 months. ① The average operation time was (132.5±21.4) min, and the intraoperative blood loss was (227.3±43.1) ml. ② Preoperative ESR was (74.56±15.43) mmol/h, and CRP was (43.21±19.37) mg/L. Seven days after surgery, ESR was (42.37±19.26) mmol/h, and CRP was (22.51±18.42) mg/L. In the last follow-up, ESR was (9.25±4.13) mmol/h, and CRP was (8.17±3.21) mg/L. They have statistically significant differences before surgery, seven days after the surgery and in the last follow-up (P<0.05). ③ The average VAS score before surgery was (7.21±1.40) points, the average JOA score was (18.24±4.13) points, the average VAS score seven days after surgery was (2.23±1.32) points, the average JOA score was (24.72±3.19) points, and the average VAS score in the last follow-up was (1.63±0.54) points. JOA score was (27.72±2.61). They have statistically significant differences before surgery, seven days after the surgery and in the last follow-up (P<0.05) ④Six patients had neurologic symptoms with ASIA grade D before surgery, 2 patients recovered to Grade E seven days after surgery, and the remaining 4 patients also recovered to grade E in the last follow-up. Besides, 26 patients were graded as Grade E before surgery, seven days after surgery and in the last follow-up. ⑤ After surgery, 1 patient suffered psoas major muscle damage, which was manifested as reduced hip flexion strength of the lower extremity, and returned to normal three weeks later. Apart from that, none of the patients had complications such as abdominal organ injury and poor wound healing. Conclusion: OLIF is a new minimally invasive method for treating lumbar tuberculosis, with short operation time, less blood loss, convenient operation, thorough removal of lesions, and good long-term follow-up effect. It is worthy of promotion and application.