bluuebunny/crossref_metadata_2025_split huggingface dataset:
Transcatheter aortic valve implantation in a 13-year-old child with end-stage heart failure: a case report
Authors: Unbehaun Axel , Kelm Marcus , Miera Oliver , Kempfert Joerg 
Published: February 2021

Abstract

Background
Left ventricular non-compaction cardiomyopathy (LVNC) has been reported in association with almost all types of congenital heart valve disease. The presence of LVNC-related ventricular dysfunction increases the perioperative risk in these patients. The advantages of transcatheter treatment modalities outweigh those of surgical strategies, as they avoid cardioplegic arrest and myocardial trauma. To our knowledge, there have been no reports on transcatheter treatment of pure aortic regurgitation in patients with a bicuspid aortic valve (BAV) and concomitant LVNC.

Case summary
In this article, we present the case of a 13-year-old boy with a regurgitant BAV and concomitant LVNC who presented with end-stage heart failure and severe pulmonary hypertension. As a bridge to definitive therapy, the patient underwent an uneventful transcatheter aortic valve implantation (TAVI) using a 26-mm balloon-expandable prosthesis. Device success without paravalvular regurgitation was achieved. At 17 months of follow-up, a steady reduction in pulmonary arterial pressure, persistent normalization of systolic left ventricular function and a tremendous improvement in the patient’s physical resilience was observed. The initially considered heart–lung transplantation was avoided and will not be necessary.

Discussion
To the best of our knowledge, this is the first case performed with TAVI for BAV regurgitation in the context of LVNC. With technical modifications and appropriate planning, TAVI in paediatric patients with a non-calcified BAV is feasible. Different imaging modalities revealed an intriguing relationship between aortic regurgitation and morphological signs of a left ventricular non-compaction myocardium.
A lacZ reporter with high activity in the human fungal pathogen Candida albicans
Authors: Klimova Natalia , Chu Siwei , Turcotte Bernard 
Published: February 2021

ABSTRACT
Reporter genes are useful tools to study gene transcription in various organisms. For example, the lacZ gene encoding β-galactosidase has been extensively used as a reporter in bacteria, budding yeast, fruit fly, mouse etc. However, use of this gene in the human fungal pathogen Candida albicans has been limited, probably due to low β-galactosidase activity. Here, we describe a reporter derived from the Vibrio cholerae lacZ gene in which codons have been optimized for expression in C. albicans. The constitutively active ACT1 promoter was fused to this synthetic lacZ reporter and integrated in the C. albicans genome. High β-galactosidase activity in liquid assays was observed for this reporter as well as coloration on X-gal plates. When the lacZ reporter expression was driven by the MET3 promoter, β-galactosidase activity in liquid assays and coloration on X-gal plates was higher in the absence of methionine, thus recapitulating the regulation of the native MET3 gene. This synthetic lacZ gene extends the toolbox of C. albicans reagents by providing a useful reporter for analysis of promoter activity in this organism of medical importance.
Foreign Policy in the Fourth Dimension (FP4D): Locating Time in Decision-Making
Authors: Beasley Ryan K , Hom Andrew R 
Published: February 2021

AbstractWhile international relations scholarship has taken a “temporal turn,” foreign policy decision-making (FPDM) research reveals little explicit theoretical attention to time. Time is an important aspect of several prominent frameworks, yet these either fail to make explicit their conception of time or fail to reflect upon the implications of their temporal assumptions and understandings. We address this lacuna by developing a timing perspective on FPDM. We present the central features of this perspective, including the nature of timing agency, temporal motivations, the timing of decision-making processes, and timing as a foreign policy tool. Illustrated with empirical examples, we show how timing plays out in FPDM and helps to shed new light on our understanding of crises and ways decision-makers may grapple with them. We conclude by considering the theoretical and empirical benefits and challenges of bridging FPDM with theoretical approaches to time.
China's “Major Country Diplomacy”: Legitimation and Foreign Policy Change
Authors: Smith Stephen N 
Published: February 2021

AbstractThis paper probes China's official political concept of “Major Country Diplomacy with Chinese Characteristics” to argue that the boundaries of legitimate state action have been dramatically expanded since Xi Jinping came to power in 2012. Building on Patrick Jackson's transactional social constructivism, I place the causal mechanism in China's new assertiveness in seminal changes to how Chinese elites legitimize their country's role in global politics. Drawing upon elite speeches, Party documents, and Chinese-language scholarship between 2013 and 2019, I show how new legitimation strategies are used to justify China's effort to proactively reform international order, engage in ideological competition with the West, and assume greater responsibility for global affairs in accordance with its elevated power and status. The boundaries of action sanctioned by this new discourse are likely to persist in the short to medium term, with implications for regional order in Asia and beyond.
A novel allele of the Arabidopsis thaliana MACPF protein CAD1 results in deregulated immune signaling
Authors: Holmes Danalyn R , Bredow Melissa , Thor Kathrin , Pascetta Sydney A , Sementchoukova Irina , Siegel Kristen R , Zipfel Cyril , Monaghan Jacqueline 
Published: February 2021

Abstract
Immune recognition in plants is governed by two major classes of receptors: pattern recognition receptors (PRRs) and nucleotide-binding leucine-rich repeat receptors (NLRs). Located at the cell surface, PRRs bind extracellular ligands originating from microbes (indicative of “non-self”) or damaged plant cells (indicative of “infected-self”), and trigger signaling cascades to protect against infection. Located intracellularly, NLRs sense pathogen-induced physiological changes and trigger localized cell death and systemic resistance. Immune responses are under tight regulation in order to maintain homeostasis and promote plant health. In a forward-genetic screen to identify regulators of PRR-mediated immune signaling, we identified a novel allele of the membrane-attack complex and perforin (MACPF)-motif containing protein CONSTITUTIVE ACTIVE DEFENSE 1 (CAD1) resulting from a missense mutation in a conserved N-terminal cysteine. We show that cad1-5 mutants display deregulated immune signaling and symptoms of autoimmunity dependent on the lipase-like protein ENHANCED DISEASE SUSCEPTIBILITY 1 (EDS1), suggesting that CAD1 integrity is monitored by the plant immune system. We further demonstrate that CAD1 localizes to both the cytosol and plasma membrane using confocal microscopy and subcellular fractionation. Our results offer new insights into immune homeostasis and provide tools to further decipher the intriguing role of MACPF proteins in plants.
Concordance Invariants and the Turaev Genus
Authors: Jung Hongtaek , Kang Sungkyung , Kim Seungwon 
Published: February 2021

Abstract
We show that the differences between various concordance invariants of knots, including Rasmussen’s $s$-invariant and its generalizations $s_n$-invariants, give lower bounds to the Turaev genus of knots. Using the fact that our bounds are nontrivial for some quasi-alternating knots, we show the additivity of Turaev genus for a certain class of knots. This leads us to the 1st example of an infinite family of quasi-alternating knots with Turaev genus exactly $g$ for any fixed positive integer $g$, solving a question of Champanerkar–Kofman.
The Role of Molecular Testing in Pediatric Meningitis Surveillance in Southern and East African Countries, 2008–2017
Authors: du Plessis Mignon , de Gouveia Linda , Freitas Cesar , Abera Negga Asamene , Lula Budiaki Sylvie , Raboba Julia Liliane , Nhantumbo Aquino Albino , Jantjies Elana , Uwimana Jeannine , Phungwayo Nomcebo , Maphalala Gugu , Masona Gilbert , Muyombe John , Mugisha David , Nalumansi Esther , Odongkara Moses , Lukwesa-Musyani Chileshe , Nakazwe Ruth , Dondo Vongai , Macharaga John , Weldegebriel Goitom G , Mwenda Jason M , Serhan Fatima , Cohen Adam L , Lessa Fernanda C , von Gottberg Anne 
Published: February 2021

Abstract

Background
As part of the global Invasive Bacterial Vaccine-Preventable Diseases Surveillance Network, 12 African countries referred cerebrospinal fluid (CSF) samples to South Africa’s regional reference laboratory. We evaluated the utility of real-time polymerase chain reaction (PCR) in detecting and serotyping/grouping Haemophilus influenzae, Neisseria meningitidis, and Streptococcus pneumoniae (HNS).

Methods
From 2008 to 2017, CSF samples collected from children &lt;5 years old with suspected meningitis underwent routine microbiology testing in-country, and 11 680 samples were submitted for HNS PCR at the regional reference laboratory. Unconditional logistic regression, with adjustment for geographic location, was performed to identify factors associated with PCR positivity.

Results
The overall HNS PCR positivity rate for all countries was 10% (1195 of 11 626 samples). In samples with both PCR and culture results, HNS PCR positivity was 11% (744 of 6747 samples), and HNS culture positivity was 3% (207 of 6747). Molecular serotype/serogroup was assigned in 75% of PCR-positive specimens (762 of 1016). Compared with PCR-negative CSF samples, PCR-positive samples were more often turbid (adjusted odds ratio, 6.80; 95% confidence interval, 5.67–8.17) and xanthochromic (1.72; 1.29–2.28), had elevated white blood cell counts (6.13; 4.71–7.99) and high protein concentrations (5.80; 4.34–7.75), and were more often HNS culture positive (32.70; 23.18–46.12).

Conclusion
PCR increased detection of vaccine-preventable bacterial meningitis in countries where confirmation of suspected meningitis cases is impeded by limited culture capacity.
Heterogeneous Models and Modelling Approaches for Engineering of Interactive Systems
Authors: Aït-Ameur Yamine , Bowen Judy , Campos José , Palanque Philippe , Weyers Benjamin 
Published: February 2021

Abstract
This editorial introduces the special issue of Interacting with Computer on Heterogeneous Models and Modelling Approaches for Engineering of Interactive Systems. This special issue was proposed to gather the best contributions from a series of workshops organized alongside conferences such as FM’19 (3rd World Congress on Formal Methods) and EICS’19 (11th ACM SIGCHI Symposium on Engineering Interactive Computing Systems). It also encompasses papers submitted directly to this special issue.
Kant on Aesthetic Ideas, Rational Ideas and the Subject-Matter of Art
Authors: Geiger Ido 
Published: February 2021

AbstractThe notion of aesthetic ideas is of great importance to Kant's thinking about art. Despite its importance, he says little about it. He characterizes aesthetic ideas as representations of the imagination and says that the gift of artistic genius is the inscrutable capacity to envision them. Furthermore, they are counterparts of rational ideas. Works of art thus sensibly present rational ideas; the pleasure they occasion is a consequence of the enriching process of reflection upon the wealth of content they sensibly present. The purpose of this article is to ask whether all rational ideas have aesthetic ideas as counterparts, and so can be presented in art, or whether only some do and which. The answer should reveal what, for Kant, is the subject-matter of art. I argue that for Kant art is concerned with the range and variety of human freedom as well as with its highest fulfillment in morality.
Local sectoral specialization in a warming world
Authors: Conte Bruno , Desmet Klaus , Nagy Dávid Krisztián , Rossi-Hansberg Esteban 
Published: February 2021

AbstractThis paper quantitatively assesses the world’s changing economic geography and sectoral specialization due to global warming. It proposes a two-sector dynamic spatial growth model that incorporates the relation between economic activity, carbon emissions and temperature. The model is taken to the data at the 1° by 1° resolution for the entire world. Over a 200-year horizon, rising temperatures consistent with emissions under Representative Concentration Pathway 8.5 push people and economic activity northwards to Siberia, Canada and Scandinavia. Compared with a world without climate change, clusters of agricultural specialization shift from Central Africa, Brazil and India’s Ganges Valley, to Central Asia, parts of China and northern Canada. Equatorial latitudes that lose agriculture specialize more in non-agriculture but, due to their persistently low productivity, lose population. By the year 2200, predicted losses in real GDP and utility are 6% and 15%, respectively. Higher trade costs make adaptation through changes in sectoral specialization more costly, leading to less geographic concentration in agriculture and larger climate-induced migration.
A new species of the genus Oligoryzomys (Rodentia: Cricetidae) from Peru
Authors: Hurtado Natali 
Published: February 2021

Abstract
The genus Oligoryzomys is the most speciose genus in the tribe Oryzomyini; however, under the general lineage concept of species, at least nine lineages are pending review and naming. In this study, I carry out qualitative and quantitative morphological analyses of specimens of Oligoryzomys andinus, O. aff. andinus, and O. arenalis, to delimit these three lineages. I show that O. aff. andinus is morphologically distinct both from O. andinus and O. arenalis, corroborating that the lineage merits recognition as a species, as suggested by previously published molecular analyses (Hurtado and D’Elía 2019). I therefore describe O. aff. andinus as a new species endemic to the Polylepis forests on the western slope of the Andes and coastal Lomas in central Peru. Finally, I propose two hypotheses about the biogeographical history of O. andinus, O. arenalis, and this new species.
Hydroxyurea Use Associated with Nonverbal and Executive Skills in Sickle Cell Anemia
Authors: Tarazi Reem A , Patrick Kristina E , Iampietro Mary , Apollonsky Nataly 
Published: February 2021

AbstractObjectiveHydroxyurea (HU) is used in children with sickle cell disease (SCD) to increase fetal hemoglobin (HF), contributing to a decrease in physical symptoms and potential protection against cerebral microvasculopathy. There has been minimal investigation into the association between HU use and cognition in this population. This study examined the relationship between HU status and cognition in children with SCD.MethodsThirty-seven children with SCD HbSS or HbS/β0 thalassaemia (sickle cell anemia; SCA) ages 4:0–11 years with no history of overt stroke or chronic transfusion completed a neuropsychological test battery. Other medical, laboratory, and demographic data were obtained. Neuropsychological function across 3 domains (verbal, nonverbal, and attention/executive) was compared for children on HU (n = 9) to those not taking HU (n = 28).ResultsChildren on HU performed significantly better than children not taking HU on standardized measures of attention/executive functioning and nonverbal skills. Performance on verbal measures was similar between groups.ConclusionsThese results suggest that treatment with HU may not only reduce physical symptoms, but may also provide potential benefit to cognition in children with SCA, particularly in regard to attention/executive functioning and nonverbal skills. Replication with larger samples and longitudinal studies are warranted.
Shoulder Pain of Spinal Source in the Military: A Case Series
Authors: Hathcock Joseph A , Boyer Chris W , Morris Jamie B 
Published: February 2021

ABSTRACT
Musculoskeletal injury (MSI) presents the greatest threat to military mission readiness. Atraumatic shoulder pain is a common military MSI that often results in persistent functional limitations. Shoulder orthopedic evaluation presents many diagnostic challenges, due in part to the possibility of a spinal source of symptoms. This case series outlines the use of mechanical diagnosis and therapy to screen the cervical and thoracic spine in active duty (AD) service members (SMs) with a chief complaint of unchanging or worsening shoulder pain. All three SMs previously received shoulder-specific diagnoses from experienced clinicians, yet repeated movements revealed a possible spinal nociceptive driver that guided targeted intervention. Treatment directed only at the cervical spine resulted in a clinically important improvement within an average of 10 days from the initial evaluation, return to duty (RTD) within an average of 32 days, and continued resolution at 3 months. SMs can independently complete the screening process with guidance from healthcare providers, ultimately shaping the treatment strategy and possibly facilitating self-management of future recurrence. This case series demonstrates that identification of shoulder pain of spinal source in the military population may be an important step in facilitating timely RTD. These cases also highlight the use of a standardized, systematic method to screen the cervical and thoracic spine that concurrently reveals the indicated treatment. Further research to determine the prevalence of shoulder pain of spinal source in the AD population and its impact on RTD rates has the potential to reduce the substantial burden of MSI in the military.
Adult-diagnosed Chronic Granulomatous Disease: The Need to Increase Awareness
Authors: Baxter Joseph , Smith Derek , Webb Charles 
Published: February 2021

ABSTRACT
Chronic granulomatous disease is genetic disorder characterized by the inability of phagocytes to produce sufficient oxidative burst needed to kill intracellular organisms. Patients have recurrent, life-threatening infections involving multiple systems including the lungs, skin, lymph nodes, and liver. The majority of patients with chronic granulomatous disease are diagnosed in childhood although some may present in adulthood due to a milder phenotype. Unfortunately, these patients may also present with concomitant autoimmune diseases. We describe a 48-year-old woman with a history of immune thrombocytopenia and systemic lupus erythematosus on immunosuppressive therapy. She developed subsequent bacterial and fungal infections initially attributed to immunosuppressive drugs. Further evaluation revealed the diagnosis of chronic granulomatous disease. We review the diagnosis and treatment of chronic granulomatous disease in hopes to increase awareness of this disease in adulthood in order to initiate potential life-saving prophylactic antibiotics.
3D1D hydro-nucleosynthesis simulations – I. Advective–reactive post-processing method and its application to H ingestion into He-shell flash convection in rapidly accreting white dwarfs
Authors: Stephens David , Herwig Falk , Woodward Paul , Denissenkov Pavel , Andrassy Robert , Mao Huaqing 
Published: February 2021

ABSTRACT
We present two mixing models for post-processing of 3D hydrodynamic simulations applied to convective–reactive i-process nucleosynthesis in a rapidly accreting white dwarf (RAWD) with [Fe/H] = −2.6, in which H is ingested into a convective He shell. A 1D advective two-stream model adopts physically motivated radial and horizontal mixing coefficients constrained by 3D hydrodynamic simulations. A simpler approach uses diffusion coefficients calculated from the same simulations. All 3D simulations include the energy feedback of the 12C(p, γ)13N reaction from the H entrainment. Global oscillations of shell H ingestion in two of the RAWD simulations cause bursts of entrainment of H and non-radial hydrodynamic feedback. With the same nuclear network as in the 3D simulations, the 1D advective two-stream model reproduces the rate and location of the H burning within the He shell closely matching the 3D simulation predictions, as well as qualitatively displaying the asymmetry of the XH profiles between the upstream and downstream. With a full i-process network the advective mixing model captures the difference in the n-capture nucleosynthesis in the upstream and downstream. For example, 89Kr and 90Kr with half-lives of $3.18\,\,\mathrm{\mathrm{min}}$ and $32.3\,\,\mathrm{\mathrm{s}}$ differ by a factor 2–10 in the two streams. In this particular application the diffusion approach provides globally the same abundance distribution as the advective two-stream mixing model. The resulting i-process yields are in excellent agreement with observations of the exemplary CEMP-r/s star CS31062-050.
Chondrules from high-velocity collisions: thermal histories and the agglomeration problem
Authors: Choksi Nick , Chiang Eugene , Connolly Harold C , Gainsforth Zack , Westphal Andrew J 
Published: February 2021

ABSTRACT
We assess whether chondrules, once-molten mm-sized spheres filling the oldest meteorites, could have formed from super-km s−1 collisions between planetesimals in the solar nebula. High-velocity collisions release hot and dense clouds of silicate vapour which entrain and heat chondrule precursors. Thermal histories of CB chondrules are reproduced for colliding bodies ∼10–100 km in radius. The slower cooling rates of non-CB, porphyritic chondrules point to colliders with radii ≳ 500 km. How chondrules, collisionally dispersed into the nebula, agglomerated into meteorite parent bodies remains a mystery. The same orbital eccentricities and inclinations that enable energetic collisions prevent planetesimals from re-accreting chondrules efficiently and without damage; thus the sedimentary laminations of the CB/CH chondrite Isheyevo are hard to explain by direct fallback of collisional ejecta. At the same time, planetesimal surfaces may be littered with the shattered remains of chondrules. The micron-sized igneous particles recovered from comet 81P/Wild-2 may have originated from in-situ collisions and subsequent accretion in the proto-Kuiper belt, obviating the need to transport igneous solids across the nebula. Asteroid sample returns from Hayabusa2 and OSIRIS-REx may similarly contain chondrule fragments.
Reprograming of sRNA target specificity by the leader peptide peTrpL in response to antibiotic exposure
Authors: Melior Hendrik , Li Siqi , Stötzel Maximilian , Maaß Sandra , Schütz Rubina , Azarderakhsh Saina , Shevkoplias Aleksei , Barth-Weber Susanne , Baumgardt Kathrin , Ziebuhr John , Förstner Konrad U , Chervontseva Zoe , Becher Dörte , Evguenieva-Hackenberg Elena 
Published: February 2021

Abstract
Trans-acting regulatory RNAs have the capacity to base pair with more mRNAs than generally detected under defined conditions, raising the possibility that sRNA target specificities vary depending on the specific metabolic or environmental conditions. In Sinorhizobium meliloti, the sRNA rnTrpL is derived from a tryptophan (Trp) transcription attenuator located upstream of the Trp biosynthesis gene trpE(G). The sRNA rnTrpL contains a small ORF, trpL, encoding the 14-aa leader peptide peTrpL. If Trp is available, efficient trpL translation causes transcription termination and liberation of rnTrpL, which subsequently acts to downregulate the trpDC operon, while peTrpL is known to have a Trp-independent role in posttranscriptional regulation of antibiotic resistance mechanisms. Here, we show that tetracycline (Tc) causes rnTrpL accumulation independently of Trp availability. In the presence of Tc, rnTrpL and peTrpL act collectively to destabilize rplUrpmA mRNA encoding ribosomal proteins L21 and L27. The three molecules, rnTrpL, peTrpL, and rplUrpmA mRNA, form an antibiotic-dependent ribonucleoprotein complex (ARNP). In vitro reconstitution of this ARNP in the presence of competing trpD and rplU transcripts revealed that peTrpL and Tc cause a shift of rnTrpL specificity towards rplU, suggesting that sRNA target prioritization may be readjusted in response to changing environmental conditions.