bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Relationship of Stress and Sleep Quality amongst Cognitive Science Students
Authors: CHEONG CAR LYN , TAN KOCK WAH 
Published: June 2021

Stress is a common problem in modern life. Most Malaysian students suffer from high stress levels and poor sleep quality without realising their harmfulness to health and well-being. The major focus of the paper is to examine the relationship between stress and sleep quality among Cognitive Science undergraduate students. This paper also investigates if demographic attributes (gender differences and year of study) affect stress level and sleep quality. The participants of this study comprised 90 Cognitive Science undergraduate students studying at the Universiti Malaysia Sarawak. Data was collected by using the Perceived Stress Questionnaire (Perceived Stress Scale) and Sleep Quality Questionnaire (Pittsburgh Sleep Quality Index). The results of the study showed that there was a weak relationship between stress and sleep quality. Those in the poor sleep category reported higher levels of perceived stress. Moreover, the findings showed that female students had higher stress levels than male students. First year students appeared to experience poorer sleep quality compared to final year students. The study also found no interaction effects between gender and year of study on sleep quality and stress. Further research could be conducted with a bigger population size and in other study programs. Future research could also assess non-demographic factors which might influence stress and sleep quality.
The political development in Sri Lanka after civil war ended: a critical review for after Zarb-e-Azb operation in Pakistan
Authors: Shabbir Taha , Naz Kehkashan 
Published: June 2021

The Sri Lankan civil war began in 1983 and lasted until 2009. The tension stems from Sri Lanka's colonial period and subsequent post-colonial policies that harmed the Tamil people. Without viable alternatives, a part of the Tamil population resorted to the degree of brutality that precipitated a second civil war. Regional, domestic, and global attempts to bring the war to a halt have been futile, though some more local measures have been active. A ruthless military campaign brought the conflict to an end. However, nothing has been done in the aftermath of the war to try to resolve the civil war, including its roots. Sri Lanka's civil war exemplifies the uncertain existence of civil war resolution. With this in mind, the war's conclusion was unquestionably the product of a strategic triumph. However, the civil war should have ended; a unique constellation of structural, state, and national forces collaborated to allow for unrestricted military aggression. As long as the dominant forces, including the United States and significant European countries, understood that enough bloodshed had happened, the country's aggression could be brought to a stop. China and India, with India abstaining, voted to support the Sri Lankan government in its major offensive against insurgents. Internationally, the newly restored government used the full might of the forces against the rebels. As a consequence, those variables are deemed unusable in other situations.
Tamil-Sinhala rivalry stretches all the way back to Sri Lanka's colonial period. The Tamil community took advantage of numerous market opportunities under British rule, which lasted from 1815 to 1948. Additionally, many group members attended school in colonial countries owing to a shortage of educational facilities in their home countries. With the exception of a few, the Sinhalese culture, on the other side, maintained its isolation from the British. As could be anticipated, the proportion of Tamils employing in the civil service, academia, and law increased dramatically following Sri Lanka's independence in 1948. Historically, the Sinhalese population has been hesitant to accept pluralism, having collaborated with the British to effect a shift of domination since the 1930s. When Sri Lanka's compulsory adult franchise was expanded to all citizens in 1931, there were no arrangements for minority rights. Tamil and Muslim community members shared discontent in the inconsistency with which their desires are pursued. T was dissatisfied with current political developments, and a large number of Tamils boycotted the elections conducted in compliance with this document. Also immediate liberty was abolished in 1947 by the Soulbury Constitution. The argument that no individual should be discriminated against on the grounds of racial origin or faith, though, proved to be a procedural impediment. Finally, in effect, it established a unitary and majoritarian state.
The Reliability and Validity Analysis of The Turkish Version of the Test to Assess the Psychological Dependence on Smoking
Authors: Bardakci Mert , Oztora Serdar , Dagdeviren Hamdi Nezih 
Published: June 2021

Aim: Psychological component of cigarette dependence is as important as its physical component. In addition to psychoactive material in the cigarette, factors such as happiness, joy, accompaniment with another activity, feeling self-confident confidence and social status play an effective role in smoking dependence. This signifies the fact that smoking dependence has to be dealt with in a multidimensional way. Test to Assess the Psychological Dependence on Smoking (TAPDS) published by Ponciano-Rodríguez et al, is developed to assess the psychological dependence on tobacco. The aim of this study iis study aims to test the reliability and validity of the Turkish version of the Test to Assess the Psychological Dependence on Smoking in the Turkish population.

Methods: The Test to Assess the Psychological Dependence on Smoking (TAPDS) Turkish Version with 25 questions along with a sociodemographic form was handed out to volunteer participants. Data was were collected from a total number of 420 voluntary participants, who were randomly selected and current smokers. Confirmatory Factor Analysis was performed and Cronbach's α and McDonald's ω coefficients were used to determine the reliability of the scores of the scale.

Results: The participants consisted of 263 males and 157 females, with an average age of 31.07 years, ranging from 20 to 85. The factor loadings of the scale for all items are observed to be more than 0.30 and all the items are statistically significant. Cronbach's α and McDonald's ω values calculated for the total score are 0.93.

Conclusion: The Turkish version of TAPDS appears to be effective for assessing psychological dependence of smoking and it can be recommended as a sufficient and highly reliable and valid scale to be used.

Keywords: smoking, validity, reliability, psychological dependence
Deciphering the in vivo Dynamic Proteomics of Mesenchymal Stem Cells in Critical Limb Ischemia
Authors: Du Yipeng , Li Xiaoting , Yan Wenying , Zeng Zhaohua , Han Dunzheng , Ouyang Hong , Pan Xiudi , Luo Bihui , Zhou Bohua , Fu Qiang , Lu Dongfeng , Huang Zheng , Li Zhiliang 
Published: June 2021

ObjectiveRegenerative therapy using mesenchymal stem cells (MSC) is a promising therapeutic method for critical limb ischemia (CLI). To understand how the cells are involved in the regenerative process of limb ischemia locally, we proposed a metabolic protein labeling method to label cell proteomes in situ and then decipher the proteome dynamics of MSCs in ischemic hind limb.Methods and ResultsIn this study, we overexpressed mutant methionyl-tRNA synthetase (MetRS), which could utilize azidonorleucine (ANL) instead of methionine (Met) during protein synthesis in MSCs. Fluorescent non-canonical amino-acid tagging (FUNCAT) was performed to detect the utilization of ANL in mutant MSCs. Mice with hindlimb ischemia (HLI) or Sham surgery were treated with MetRSmut MSCs or PBS, followed by i.p. administration of ANL at days 0, 2 6, and 13 after surgery. FUNCAT was also performed in hindlimb tissue sections to demonstrate the incorporation of ANL in transplanted cells in situ. At days 1, 3, 7, and 14 after the surgery, laser doppler imaging were performed to detect the blood reperfusion of ischemic limbs. Ischemic tissues were also collected at these four time points for histological analysis including HE staining and vessel staining, and processed for click reaction based protein enrichment followed by mass spectrometry and bioinformatics analysis. The MetRSmut MSCs showed strong green signal in cell culture and in HLI muscles as well, indicating efficient incorporation of ANL in nascent protein synthesis. By 14 days post-treatment, MSCs significantly increased blood reperfusion and vessel density, while reducing inflammation in HLI model compared to PBS. Proteins enriched by click reaction were distinctive in the HLI group vs. the Sham group. 34, 31, 49, and 26 proteins were significantly up-regulated whereas 28, 32, 62, and 27 proteins were significantly down-regulated in HLI vs. Sham at days 1, 3, 7, and 14, respectively. The differentially expressed proteins were more pronounced in the pathways of apoptosis and energy metabolism.ConclusionIn conclusion, mutant MetRS allows efficient and specific identification of dynamic cell proteomics in situ, which reflect the functions and adaptive changes of MSCs that may be leveraged to understand and improve stem cell therapy in critical limb ischemia.
Inferring Hypogene Karst at Depth from the Patterns of Non-Tectonic Syncline Networks in Eocene Limestones, Western Desert, Egypt
Authors: Tewksbury Barbara J. , Tarabees Elhamy A. , Welch Robert M. , Mehrtens Charlotte J. 
Published: June 2021

Indirect indicators are critically important for recognizing hypogene karst that is too deep-seated to have explorable hypogene caves. We have suggested in previous publications that an extensive network of non-tectonic synclines in otherwise flat-lying Eocene limestone in Egypt might be such an indirect indicator. We proposed that synclines formed by sag of limestone layers overlying a zone of hypogene karst that today remains deep below the surface and suggested that hypogene speleogenesis resulted from ascending aggressive fluids associated with crustal extension and magmatism in Egypt during Red Sea Rift initiation. Without hypogene caves to explore, however, we were unable to provide compelling evidence for hypogene karst processes. By doubling our mapping area from 4,000 to 8,000 km2, a clear picture has emerged of patterns in the syncline network that provide compelling evidence for hypogene speleogenesis. Over this larger area, the network displays two distinct patterns: 1) synclines and ridges that outline polygons 700–2,000 m across, and 2) narrow N–S zones of synclines spaced 5–10 km apart, with WNW–ESE to NW–SE trending shallow synclines and ridges traversing the panels between N–S zones. The geometries suggest that the syncline network is controlled by two structural patterns in rocks underlying the limestones: 1) polygonal faults in underlying shales and 2) reactivated N–S, left-lateral basement faults that are largely blind at the current level of erosion. These structures served as conduits that conveyed fluids upward into the overlying Eocene limestones, triggering dissolution at depth and a pattern of sag above that was inherited from the nature and pattern of faults and fractures in rocks underlying the limestones. The unique patterns and characteristics of this network of synclines are applicable elsewhere as an indirect indicator of deep-seated hypogene karst. Our new data also strongly suggest that syncline formation spanned the time of crustal extension in Egypt associated with onset of Red Sea rifting ∼23–22 Ma. Endogenic CO2 associated with mantle-derived basaltic magmas was likely a significant component of fluids, perhaps involving highly aggressive supercritical CO2. Mantle-derived C and He in modern Egyptian oasis water suggest that hypogene speleogenesis may still be locally active.
Granulocyte-Macrophage Colony Stimulating Factor in Single Blastocyst Conditioned Medium as a Biomarker for Predicting Implantation Outcome of Embryo
Authors: Chen Peilin , Huang Chunyu , Sun Qing , Zhong Huixian , Xiong Feng , Liu Su , Yao Zhihong , Liu Zhiqiang , Wan Caiyun , Zeng Yong , Diao Lianghui 
Published: June 2021

BackgroundIt is highly desirable to develop new strategies based on secretomics to more accurately selection of embryos with the highest developmental potential for transfer. Granulocyte-macrophage colony-stimulating factor (GM-CSF) has been reported to promote embryo development and pregnancy establishment. However, the predictive value of GM-CSF in single blastocyst selection remains unclear. This study is to determine the concentration of GM-CSF in human single-blastocyst conditioned medium (SBCM) and to evaluate its association with embryo quality and pregnancy outcome.MethodsThe patients with ≤38 years of age receiving the first cycle of assisted reproductive therapy were included in this study. The patients who had &lt;4 top-quality embryos formed by the fertilized two pronuclear zygotes on day 3 were excluded. A total of 126 SBCM samples (SBCMs) were included, of which blastocysts from 77 SBCMs were later transferred in subsequent frozen-thawed embryo transfer. The concentrations of GM-CSF were detected by single-molecule array (SIMOA) and analyzed for their possible association with embryo quality and pregnancy outcomes. The top-quality embryo (TQ), positive HCG (HP), clinical pregnancy (CP), and ongoing pregnancy (OP) rates were determined and compared between groups divided based on GM-CSF concentrations.ResultsThe detection rate of GM-CSF was found to be 50% in all SBCMs. There were significant differences in TQ rate, HP rate, CP rate and OP rate among high concentration group, medium concentration group and low concentration group. Both GM-CSF alone or GM-CSF combined with the morphological score (MS) had a greater AUC of ROC curve than that of MS alone to predict the pregnancy outcome, and GM-CSF combined with MS had the highest AUC.ConclusionsThe concentration of GM-CSF in SBCM was detected at fg/ml levels, which was associated with embryo quality and pregnancy outcome. Collectively, GM-CSF may be used as a biomarker for prediction of pregnancy outcome and selection of embryos with high developmental potential for transfer in assisted reproductive technology (ART).
Nanoglass and Nanocrystallization Reactions in Metallic Glasses
Authors: Perepezko John H , Gao Meng , Wang Jun-Qiang 
Published: June 2021

Strategies to change the properties of metallic glass by controlling the crystallization and the glass transition behavior are essential in promoting the application of these materials. Aside from changing the composition approaches to stabilize the glass and frustrate the nucleation and growth of crystals, new strategies at a fixed glass composition are of special interest. In this review, some recent work is summarized on new strategies to tune the properties of metallic glasses without changing composition. First, the nanocrystallization strategy is introduced that is based on the nanocrystallized microstructures such as those that develop in marginal Al-based metallic glasses. The heterogeneous and transient nucleation effects in the nanocrystallization reactions in Al-based metallic glasses are systematically investigated and can be assessed by the determination of delay time based on Flash DSC measurements. These results provide a basis to understand the strong effect of minor alloying additions on the onset of primary Al nanocrystallization and to design the novel Al-based composites with improved properties. Secondly, by an optimal annealing treatment, a liquid-cooled Au-based metallic glass can achieve very high kinetic stability to yield a large increase in glass transition temperature of 28 K and this is 3-5 times larger than the increase usually reported. The measured enthalpy decrease is about 50% of the difference between the as-cooled glass and the equilibrium crystalline state and reaches the extrapolated enthalpy of the supercooled liquid. Finally, the nano-glass strategy makes an Au-based nanoglass show ultrastable kinetic characters at low heating rate (e.g., 300 K/s) compared to a melt-spun ribbon, which is attributed to the kinetic constraint effect of nanoglobular interfaces. These results indicate that the nanoglass microstructure can act to increase metallic glass stability and provide another mechanism for the synthesis of ultrastable glass. These developments open new opportunities to improve the stability and properties and largely increase the application potentials of metallic glasses.
Structural and Transport Properties of 1T-VSe2 Single Crystal Under High Pressures
Authors: Song Dongqi , Zhou Ying , Zhang Min , He Xinyi , Li Xinjian 
Published: June 2021

Two-dimensional transition metal dichalcogenide 1T-VSe2 exhibits a unique three-dimensional charge density wave (CDW) order below ∼110 K at ambient pressure, which shows unusual evolution under pressure. Here we report on the high-pressure structural and transport properties of 1T-VSe2 by extending the pressure up to 57.8 GPa, through electrical transport, synchrotron X-ray diffraction (XRD) and Raman scattering measurements, which unravel two critical pressure points. The CDW transition is found to be enhanced under compression at a rate of 16.5 K/GPa up to the first critical pressure PC1 ∼ 12 GPa, at which a structural phase transition from hexagonal P-3m1 to monoclinic C2/m phase takes place. The second critical pressure PC2 ∼ 33 GPa corresponds to another structural transition from monoclinic C2/m to P21/m phase. These findings extend the phase diagram of pressurized 1T-VSe2 and may help to understand pressure tuning of structures in transition metal dichalcogenides.
Attentional Bias Modification Training for Itch: A Proof-of-Principle Study in Healthy Individuals
Authors: van Laarhoven Antoinette I. M. , Becker Jennifer M. , van Ryckeghem Dimitri M. L. , Van Damme Stefaan , Crombez Geert , Wiers Reinout W. H. J. 
Published: June 2021

Itch draws our attention to allow imposing action against bodily harm (e.g., remove insects). At the same time, itch is found to interfere with ongoing tasks and daily life goals. Despite the key role of attention in itch processing, interventions that train individuals to automatically disengage attention from itch cues are lacking. The present proof-of-principle attention bias modification (ABM) training study was aimed at investigating whether attention to itch as well as sensitivity to mild itch can be changed. Healthy volunteers were randomized over three ABM-training conditions. Training was done via a modified pictorial dot-probe task. In particular, participants were trained to look away from itch stimuli (n= 38), toward itch stimuli (n= 40) or not trained toward or away from itch at all (sham training,n= 38). The effects of the ABM-training were tested primarily on attention to itch pictures. Secondarily, it was investigated whether training effects generalized to alterations in attention to itch words and mechanical itch sensitivity. The ABM-training did not alter attention toward the itch pictures, and there was no moderation by baseline levels of attention bias for itch. Also, attention bias to the itch words and itch sensitivity were not affected by the ABM-training. This study was a first step toward trainings to change attention toward itch. Further research is warranted to optimize ABM-training methodology, for example increasing motivation of participants. Eventually, an optimized training could be used in patient populations who suffer most from distraction by their symptoms of itch.Clinical Trial Registration:Identifier: NL6134 (NTR6273). The website URL is:https://www.trialregister.nl/
Enzalutamide-Induced Upregulation of PCAT6 Promotes Prostate Cancer Neuroendocrine Differentiation by Regulating miR-326/HNRNPA2B1 Axis
Authors: Liu Bo , Jiang Hui-Yang , Yuan Tao , Luo Jie , Zhou Wei-Dong , Jiang Qi-Quan , Wu Denglong 
Published: June 2021

Our previous studies have demonstrated that Enzalutamide-induced upregulation of long non-coding RNA p21 (lncRNA-p21) facilitates prostate cancer (PCa) neuroendocrine differentiation (NED). Given the important role of lncRNAs in PCa pathogenesis, and given that lots of lncRNAs are dys-regulated in neuroendocrine PCa (NEPC) patients, we next explored the biological function and underlying mechanism of lncRNA-PCAT6 (PCAT6) in mediating Enzalutamide-induced NED. The level of PCAT6 in Enzalutamide-treated PCa cells and NEPC samples were assessed using quantitative RT-PCR (qPCR). The effect of PCAT6 on PCa cell proliferation, invasion, and NED was evaluated through CCK-8, transwell, qPCR, western blot analysis, Xenograft mouse model, and in vivo lung metastasis model. We found that PCAT6 was highly expressed in NE-like cells (PC3, DU145, and NCI-H660) compared with androgen-sensitive LNCaP cells. PCAT6 was also highly expressed in NEPC tissues. Enzalutamide treatment resulted in a significant increase of PCAT6 level in a dose- and time-dependent fashion. Functionally, PCAT6 overexpression promoted NED of C4-2 cells, as evidenced by an increased expression of NE markers (NSE, ChgA, and SYP), whereas PCAT6 knockdown in NCI-H661 cells repressed NED. Furthermore, PCAT6 overexpression promoted PCa cell proliferation and invasion in vitro and in vivo. Mechanistically, PCAT6 functioned as competing endogenous (ce) RNA via absorbing miR-326, thus resulting in a de-suppression of Hnrnpa2b1 target gene. The current results demonstrate that PCAT6 acted as a tumor activator in PCa progression by sponging miR-326 and increasing Hnrnpa2b1 expression and that the PCAT6/miR-326/Hnrnpa2b1 signaling might be a new therapeutic target for PCa.
A Prospective Study on Fertility Preservation in Prepubertal and Adolescent Girls Undergoing Hematological Stem Cell Transplantation
Authors: Wikander Ida , Lundberg Frida E. , Nilsson Hanna , Borgström Birgit , Rodriguez-Wallberg Kenny A. 
Published: June 2021

BackgroundHematological stem cell transplantation (HSCT) is an established method which has markedly increased the survival rate of hematologic malignancies since its introduction in the 1980’s. The conditioning for HSCT has known gonadotoxic effects and often leads to premature loss of fertility. In this study we have prospectively followed a cohort of girls undergoing HSCT and studied the outcomes of fertility preservation treatments performed before or after HSCT, as well as the long-term reproductive outcome.MethodsIn this one-center prospective study, 39 girls counselled for fertility preservation prior to or after conditioning for HSCT for malignant or benign diseases at childhood or adolescence between 1990 and 2017 were included. The patients were presented with the option to undergo cryopreservation of ovarian tissue or oocytes depending on their age and the time available. Follicle counts of the ovarian tissue and number of oocytes collected before or after HSCT were compared between patients treated for benign and malignant diseases. Hormone measurements post HSCT treatment, including FSH and AMH, reproductive outcomes and overall survival until January 2021 were investigated.ResultsIn total, 34 girls and adolescents underwent fertility preservation before or after HSCT. Before HSCT, ovarian tissue was cryopreserved in 15 patients and two patients had oocytes preserved. Thirteen patients cryopreserved ovarian tissue after HSCT and seven patients returned to cryopreserve oocytes. Follicles were present in all tissue samples collected prior to HSCT, and in more than half of the samples collected post-HSCT. Half of the patients had spontaneous menarche or resumed menstruation post HSCT. Overall, 35 patients had survived at end of follow up and 7 patients had achieved parenthood.ConclusionsSince fertility loss is common following HSCT, fertility preservation should be offered to all patients. Fertility preservation treatments can be performed both before and after HSCT.Clinical Trial Registrationhttps://clinicaltrials.gov/show/NCT04602962, identifier NTC04602962