bluuebunny/crossref_metadata_2025_split huggingface dataset:
Opposing Effects of Granulocyte Colony-Stimulating Factor on the Initiation and Progression of Breast Cancer Bone Metastases
Authors: Hiraga Toru , Ito Susumu , Mizoguchi Toshihide 
Published: August 2021

Abstract

Granulocyte colony stimulating factor (G-CSF), an essential cytokine regulating granulopoiesis, is expressed in a substantial proportion of breast cancers, and it has been implicated in cancer progression. Here, we examined effects of G-CSF on the development of bone metastases of breast cancer using immunocompetent mouse models. The expression of CXC chemokine ligand 12 (CXCL12) in bone marrow stromal cells, which plays a critical role in the maintenance of hematopoietic stem cells and also in cancer cell homing to bone, was markedly decreased in mice treated with G-CSF. Flow cytometric analysis revealed that pretreatment of mice with G-CSF reduced the number of bone-homing cancer cells. G-CSF also increased the population of myeloid-derived suppressor cells (MDSCs) in bone marrow. Depletion of MDSCs using anti–Gr-1 antibody treatment significantly decreased the metastatic tumor burden in bone. The overall effects of G-CSF on bone metastases were finally examined using two different treatment protocols. When mice were treated with G-CSF prior to the tumor cell inoculation, G-CSF did not change bone metastatic-tumor burden. In contrast, when G-CSF treatment was started after the tumor cells had homed to bone, G-CSF significantly accelerated bone metastases formation. These results suggest that G-CSF suppressed cancer cell homing to bone by downregulating CXCL12 expression in bone marrow stromal cells, whereas G-CSF stimulated the progression of bone metastases at least in part by MDSC-mediated mechanisms.

Implications:
G-CSF had opposing effects on the initiation and progression of bone metastases of breast cancer and the balance may regulate the metastatic tumor burden.
Identification of a Novel FUS/ETV4 Fusion and Comparative Analysis with Other Ewing Sarcoma Fusion Proteins
Authors: Boone Megann A. , Taslim Cenny , Crow Jesse C. , Selich-Anderson Julia , Watson Mike , Heppner Peter , Hamill James , Wood Andrew C. , Lessnick Stephen L. , Winstanley Mark 
Published: August 2021

Abstract

Ewing sarcoma is a pediatric bone cancer defined by a chromosomal translocation fusing one of the FET family members to an ETS transcription factor. There have been seven reported chromosomal translocations, with the most recent reported over a decade ago. We now report a novel FET/ETS translocation involving FUS and ETV4 detected in a patient with Ewing sarcoma. Here, we characterized FUS/ETV4 by performing genomic localization and transcriptional regulatory studies on numerous FET/ETS fusions in a Ewing sarcoma cellular model. Through this comparative analysis, we demonstrate significant similarities across these fusions, and in doing so, validate FUS/ETV4 as a bona fide Ewing sarcoma translocation. This study presents the first genomic comparison of Ewing sarcoma–associated translocations and reveals that the FET/ETS fusions share highly similar, but not identical, genomic localization and transcriptional regulation patterns. These data strengthen the notion that FET/ETS fusions are key drivers of, and thus pathognomonic for, Ewing sarcoma.

Implications:
Identification and initial characterization of the novel Ewing sarcoma fusion, FUS/ETV4, expands the family of Ewing fusions and extends the diagnostic possibilities for this aggressive tumor of adolescents and young adults.
Future Summer Drying in the U.S. Corn Belt and the Role of Midlatitude Storm Tracks
Authors: Ting Mingfang , Seager Richard , Li Cuihua , Liu Haibo , Henderson Naomi 
Published: August 2021

AbstractDuring the summer, the Midwest United States, which covers the main US corn belt, has a net loss of surface water as evapotranspiration exceeds precipitation. The net moisture gain into the atmosphere is transported out of the region to northern high latitudes through transient eddy moisture fluxes. How this process may change in the future is not entirely clear despite the fact that the corn belt region is responsible for a large portion of the global supply of corn and soybeans. We find that increased CO2 and the associated warming increases evapotranspiration. while precipitation reduces in the region leading to further reduction in precipitation minus evaporation (P-E) in the future. At the same time, the poleward transient moisture flux increases leading to enhanced atmospheric moistures export from the corn belt region. However, storm track intensity is generally weakened in the summer due to reduced north-south temperature gradient associated with amplified warming in the midlatitudes. The intensified transient eddy moisture transport as storm track weakens can be reconciled by the stronger mean moisture gradient in the future. This is found to be caused by the climatological low-level jet transporting more moisture into the Great Plains region due to the thermodynamic mechanism under warmer conditions. Our results, for the first time, show that in the future, the US Midwest corn belt will experience more hydrological stress due to intensified transient eddy moisture export leading to drier soils in the region.
A Comparison of Tropical Cyclone Projections in a High-resolution Global Climate Model and from Downscaling by Statistical and Statistical-deterministic Methods
Authors: Jing Renzhi , Lin Ning , Emanuel Kerry , Vecchi Gabriel , Knutson Thomas R. 
Published: August 2021

AbstractIn this study, we investigate the response of tropical cyclones (TCs) to climate change by using the Princeton environment-dependent probabilistic tropical cyclone (PepC) model and a statistical-deterministic method to downscale TCs using environmental conditions obtained from the Geophysical Fluid Dynamics Laboratory (GFDL) High-resolution Forecast-oriented Low Ocean Resolution (HiFLOR) model, under the Representative Concentration Pathway 4.5 (RCP4.5) emissions scenario for the North Atlantic basin. The downscaled TCs for the historical climate (1986-2005) are compared with those in the mid- (2016-35) and late-twenty-first century (2081-2100). The downscaled TCs are also compared with TCs explicitly simulated in HiFLOR. We show that while significantly more storms are detected in HiFLOR towards the end of the twenty-first century, the statistical-deterministic model projects a moderate increase in TC frequency, and PepC projects almost no increase in TC frequency. The changes in storm frequency in all three datasets are not significant in the mid-twenty-first century. All three project that storms will become more intense and the fraction of major hurricanes and Category 5 storms will significantly increase in the future climates. However, HiFLOR projects the largest increase in intensity while PepC projects the least. The results indicate that HiFLOR’s TC projection is more sensitive to climate change effects and statistical models are less sensitive. Nevertheless, in all three datasets, storm intensification and frequency increase lead to relatively small changes in TC threat as measured by the return level of landfall intensity.
Effects of Tropical Sea Surface Temperature Variability on Northern Hemisphere Tropical Cyclone Genesis
Authors: Li Shuo , Mei Wei , Xie Shang-Ping 
Published: August 2021

Abstract
This study quantifies the contributions of tropical sea surface temperature (SST) variations during the boreal warm season to the interannual-to-decadal variability in tropical cyclone genesis frequency (TCGF) over the Northern Hemisphere ocean basins. The first seven leading modes of tropical SST variability are found to affect basinwide TCGF in one or more basins, and are related to canonical El Niño–Southern Oscillation (ENSO), global warming (GW), the Pacific meridional mode (PMM), Atlantic multidecadal oscillation (AMO), Pacific decadal oscillation (PDO), and the Atlantic meridional mode (AMM). These modes account for approximately 58%, 50%, and 56% of the variance in basinwide TCGF during 1969–2018 over the North Atlantic (NA), northeast Pacific (NEP), and northwest Pacific (NWP) Oceans, respectively. The SST effect is weak on TCGF variability in the north Indian Ocean. The SST modes dominating TCGF variability differ among the basins: ENSO, the AMO, AMM, and GW are dominant for the NA; ENSO and the AMO for the NEP; and the PMM, interannual AMO, and GW for the NWP. A specific mode may have opposite effects on TCGF in different basins, particularly between the NA and NEP. Sliding-window multiple linear regression analyses show that the SST effects on basinwide TCGF are stable in time in the NA and NWP, but have strengthened since the 1990s in the NEP. The SST effects on local TC genesis and occurrence frequency are also explored, and the underlying physical mechanisms are examined by diagnosing a genesis potential index and its components.
Contralateral radiculopathy after unilateral transforaminal lumbar interbody fusion: causes and prevention
Authors: Chen Yun-lin , Hu Xu-dong , Wang Yang , Jiang Wei-yu , Ma Wei-hu 
Published: August 2021

Background Unilateral transforminal lumbar interbody fusion (TLIF) with a single cage can provide circumferential fusion and biomechanical stability. However, the causes and prevention of contralateral radiculopathy following unilateral TLIF remain unclear. Methods In total, 190 patients who underwent unilateral TLIF from January 2017 to January 2019 were retrospectively reviewed. Radiological parameters including lumbar lordosis, segmental angle, anterior disc height, posterior disc height (PDH), foraminal height (FH), foraminal width, and foraminal area (FA) were measured preoperatively and postoperatively. Preoperative and postoperative visual analog scale scores were also recorded. Results The incidence of contralateral radiculopathy after unilateral TLIF was 5.3% (10/190). The most common cause was contralateral foraminal stenosis. Unilateral TLIF could increase the lumbar lordosis, segmental angle, and anterior disc height but decrease the PDH, FA, and FH in patients with symptomatic contralateral radiculopathy. The intervertebral cage should be placed to cover the epiphyseal ring and cortical compact bone of the midline, and the disc height can be increased to enlarge the contralateral foramen. Conclusion The most common cause of contralateral radiculopathy is contralateral foraminal stenosis. Careful preoperative planning is necessary to achieve satisfactory outcomes. Improper unilateral TLIF will decrease the PDH, FA, and FH, resulting in contralateral radiculopathy.
PKR deficiency alleviates pulmonary hypertension via inducing inflammasome adaptor ASC inactivation
Authors: Li Yapei , Li Ying , Li Lijun , Yin Minghui , Wang Jiangang , Li Xiaohui 
Published: August 2021

Pulmonary hypertension is a progressive fatal disease that currently has no specific therapeutic approaches. In this study, dsRNA‐dependent protein kinase (PKR) was considered a candidate molecule in pulmonary hypertension. We demonstrated that PKR is activated in the endothelium of experimental pulmonary hypertension models. Deletion of PKR or treatment with the PKR activation inhibitor C16 inhibited the development of pulmonary hypertension. To explore the mechanism of PKR in pulmonary hypertension, we detected its downstream signaling and found that PKR knockout represses apoptosis‐associated speck‐like protein containing CARD (ASC) activation to inhibit high mobility group box 1 (HMGB1) and interleukin‐1 beta release. To further explore whether ASC mediates the pro‐pulmonary hypertension role of PKR, we used ASC deletion mice and found that ASC deletion inhibits the development of pulmonary hypertension and the release of HMGB1 and interleukin‐1 beta. Furthermore, we co‐cultured pulmonary arterial endothelial cells (PAECs) and pulmonary arterial smooth muscle cells (PASMCs) and found that endothelial PKR promotes PASMCs proliferation through the release of HMGB1 and interleukin‐1 beta. In conclusion, these data indicate that endothelial PKR promotes the excessive proliferation of PASMCs by inducing ASC activation to release HMGB1 and interleukin‐1 beta, which lead to the development of pulmonary hypertension. Our study will provide a novel insight that PKR is a potential target in the future treatment of pulmonary hypertension.
Effect of maternal near miss on neonatal mortality in selected hospitals: Prospective cohort study, Southeast Ethiopia
Authors: Aliyi Ahmednur Adem , Deyessa Negussie , Dilnessie Mengistu Yilma 
Published: August 2021

Objective: The objective of this study was to assess effect of maternal near miss on neonatal mortality. Methods: Prospective cohort study was conducted on 384 pregnant women who came for delivery to purposely selected hospitals. The cohort was made up of 128 exposed (near miss) mothers and 256 non-exposed (non-near-miss) mothers. Women who came for delivery were only included. Those who came for services other than delivery such as abortion care, women who developed life-threatening condition not related to delivery, and those who come from no phone network area were excluded. A purposive sampling technique was used by including all mothers with near miss consecutively until the required sample size was obtained. Two non-near-miss mothers were selected using lottery for every near-miss mother. Survival analysis was done for both groups using Cox regression to look for effect of maternal near miss on neonatal mortality. Verbal informed consent from study participants was obtained. Results: A total of 354 (118 with near miss and 236 without near miss) women completed the follow-up time, yielding response rate of 92.2%. Of all, 55 (15.5%) of them have previous history of abortion, 44 (12.4%) were admitted to the intensive care unit during delivery, and 22 (6.2%) have history of past delivery of still birth. Severe preeclampsia with intensive care unit admission and severe anemia with transfusion of greater than 2 units of blood were common complications leading to maternal near miss. There were 17 (48 per 1000 live birth) neonatal death at the end of the study, of which 15 occurred among mothers with near miss. Monthly income (adjusted hazard ratio = 998, 95% confidence interval = 0.996–0.999), fetal presentation (adjusted hazard ratio = 6.48, 95% confidence interval = 1.84–22.73), APGAR score (adjusted hazard ratio = 0.746, 95% confidence interval = 0.620–0.898), and being near miss mother (adjusted hazard ratio = 8.40, 95% confidence interval = 1.638–43.118) were significantly affecting neonatal mortality. Conclusion: Maternal near miss and other fetal and general maternal characteristics have effect on occurrence of neonatal mortality. Therefore, due attention should be given to these factors for improvement of neonatal survival.
Energy-Optimal Braking Control Using a Double-Layer Scheme for Trajectory Planning and Tracking of Connected Electric Vehicles
Authors: Dong Haoxuan , Zhuang Weichao , Yin Guodong , Xu Liwei , Wang Yan , Wang Fa’an , Lu Yanbo 
Published: August 2021

AbstractMost researches focus on the regenerative braking system design in vehicle components control and braking torque distribution, few combine the connected vehicle technologies into braking velocity planning. If the braking intention is accessed by the vehicle-to-everything communication, the electric vehicles (EVs) could plan the braking velocity for recovering more vehicle kinetic energy. Therefore, this paper presents an energy-optimal braking strategy (EOBS) to improve the energy efficiency of EVs with the consideration of shared braking intention. First, a double-layer control scheme is formulated. In the upper-layer, an energy-optimal braking problem with accessed braking intention is formulated and solved by the distance-based dynamic programming algorithm, which could derive the energy-optimal braking trajectory. In the lower-layer, the nonlinear time-varying vehicle longitudinal dynamics is transformed to the linear time-varying system, then an efficient model predictive controller is designed and solved by quadratic programming algorithm to track the original energy-optimal braking trajectory while ensuring braking comfort and safety. Several simulations are conducted by jointing MATLAB and CarSim, the results demonstrated the proposed EOBS achieves prominent regeneration energy improvement than the regular constant deceleration braking strategy. Finally, the energy-optimal braking mechanism of EVs is investigated based on the analysis of braking deceleration, battery charging power, and motor efficiency, which could be a guide to real-time control.
MoS2 with Controlled Thickness for Electrocatalytic Hydrogen Evolution
Authors: Xu Xiaoxuan , Liu Lei 
Published: August 2021

AbstractMolybdenum disulfide (MoS2) has moderate hydrogen adsorption free energy, making it an excellent alternative to replace noble metals as hydrogen evolution reaction (HER) catalysts. The thickness of MoS2 can affect its energy band structure and interface engineering, which are the avenue way to adjust HER performance. In this work, MoS2 films with different thicknesses were directly grown on the glassy carbon (GC) substrate by atomic layer deposition (ALD). The thickness of the MoS2 films can be precisely controlled by regulating the number of ALD cycles. The prepared MoS2/GC was directly used as the HER catalyst without a binder. The experimental results show that MoS2 with 200-ALD cycles (the thickness of 14.9 nm) has the best HER performance. Excessive thickness of MoS2 films not only lead to the aggregation of dense MoS2 nanosheets, resulting in reduction of active sites, but also lead to the increase of electrical resistance, reducing the electron transfer rate. MoS2 grown layer by layer on the substrate by ALD technology also significantly improves the bonding force between MoS2 and the substrate, showing excellent HER stability.
Understanding the determinants of COVID-19 vaccination intention and willingness to pay: findings from a population-based survey in Bangladesh
Authors: Banik Rajon , Islam Md. Saiful , Pranta Mamun Ur Rashid , Rahman Quazi Maksudur , Rahman Mahmudur , Pardhan Shahina , Driscoll Robin , Hossain Sahadat , Sikder Md. Tajuddin 
Published: August 2021

Abstract
Background
Several coronavirus disease (COVID-19) vaccines have already been authorized and distributed in different countries all over the world, including Bangladesh. Understanding public acceptance of such a novel vaccine is vital, but little is known about the topic.

Objectives
This study aimed to investigate the determinants of intention to receive a COVID-19 vaccine and willingness to pay (WTP) among people in Bangladesh.

Methods
An anonymous and online-based survey of Bangladeshi people (mean age = 29.96 ± 9.15 years; age range = 18–60 years) was conducted using a self-reported questionnaire consisting of socio-demographics, COVID-19 experience, and vaccination-related information as well as the health belief model (HBM). Multivariable logistic regression was performed to determine the factors influencing COVID-19 vaccination intent and WTP.

Results
Of the 894 participants, 38.5% reported a definite intention to receive a COVID-19 vaccine, whereas 27% had a probable intention, and among this intent group, 42.8% wanted to get vaccinated as soon as possible. Older age, feeling optimistic about the effectiveness of COVID-19 vaccination, believing that vaccination decreases worries and risk of COVID-19 infection, and being less concerned about side effects and safety of COVID-19 vaccination under the HBM construct were found to be significant factors in COVID-19 vaccination intention. Most of the participants (72.9%) were willing to pay for a COVID-19 vaccine, with a median (interquartile range [IQR]) amount of BDT 400/US$ 4.72 (IQR; BDT 200–600/US$ 2.36–7.07) per dose. Factors associated with higher WTP were younger age, being male, having higher education, residing in an urban area, having good self-rated health status, positivity towards COVID-19 vaccination's effectiveness, and being worried about the likelihood of getting infected with COVID-19. Participants who were COVID-19 vaccination intent preferred an imported vaccine over a domestically-made vaccine (22.9% vs. 14.8%), while 28.2% preferred a routine immunization schedule.

Conclusion
The findings indicate a considerable proportion of Bangladeshi people intended to get vaccinated and had WTP for the COVID-19 vaccine. However, urgent education and awareness programs are warranted to alleviate public skepticism regarding the COVID-19 vaccination.
Infectious keratitis after corneal crosslinking for keratoconus caused by levofloxacin-resistant microorganisms
Authors: Kato Naoko , Ide Takeshi , Kobashi Hidenaga , Toda Ikuko 
Published: August 2021

Abstract
Introduction
We present seven cases of infectious keratitis after corneal crosslinking (CXL) to attenuate keratoconus progression.

Methods
Of 524 consecutive patients who underwent CXL, 7 cases (4 males and 3 females; 21.5 ± 7.1 years) developed postoperative infectious keratitis were retrospectively reviewed. CXL was performed using the Dresden protocol or an accelerated protocol involving epithelial removal.

Results
All cases appeared normal on the day after surgery, but subsequently developed eye pain, blurred vision, corneal infiltration, inflammation of the anterior chamber, and ciliary injection on day 2 or 3. Methicillin-resistant Staphylococcus aureus was cultured from two eyes, methicillin-sensitive Staphylococcus aureus from two eyes, and Streptococcus pneumoniae from one eye. All detected bacteria were resistant to levofloxacin (LVFX). Five of the seven cases, especially four of the five severe cases with hypopyon, had a history of atopic dermatitis. All cases were observed after 2015.

Conclusions
Infectious keratitis after CXL caused by microbes resistant to LVFX is increasing. In addition to careful postoperative observation of the cornea, preoperative evaluation of bacteria within the conjunctival sac evident on nasal swab cultures may be useful to identify potentially problematic microbes and inform the selection of appropriate antibiotics.