bluuebunny/crossref_metadata_2025_split huggingface dataset:
Lower Bird Evenness and Diversity Are Associated With Higher Usutu Prevalence in Culex pipiens Mosquitoes
Authors: Rodriguez‐Valencia Victor , Olive Marie‐Marie , Le Goff Gilbert , Faisse Marine , Paupy Christophe , Roiz David 
Published: February 2025

ABSTRACTIntroductionThe mosquito‐transmitted Usutu virus has spread in the last few years, becoming endemic in several areas of Europe, such as in the southern French region of the Camargue. Our aim was to study the relationships between the presence of the viral agent in Culex mosquitoes and the structure of bird communities in the context of the dilution effect.MethodsWe carried out mosquito and bird censuses in several selected localities across a land‐use gradient and screened mosquito pools for flaviviruses. We focused on exploring how host bird diversity, richness, abundance and evenness were associated with Usutu detection in Cx. pipiens.ResultsUsutu virus was detected in seven pools of Cx. pipiens, and phylogenetic analysis identified Usutu lineage Africa 3, confirming its circulation. The probability of detection in mosquitoes is associated with areas with lower bird evenness and diversity but higher bird abundance and richness and higher Cx. pipiens abundances.ConclusionsBird evenness was the variable with the greatest explanatory power, being negatively related to the probability of detecting Usutu in Cx. pipiens, supporting a dilution effect. These results will help us better understand the relationships between bird community structure and the risk of Usutu mosquito‐borne disease.
Euler characteristics of affine ADE Nakajima quiver varieties via collapsing fibres
Authors: Bertsch Lukas , Gyenge Ádám , Szendrői Balázs 
Published: February 2025

AbstractWe prove a universal substitution formula that compares generating series of Euler characteristics of Nakajima quiver varieties associated with affine ADE diagrams at generic and at certain non‐generic stability conditions via a study of collapsing fibres in the associated variation of GIT map, unifying and generalising earlier results of the last two authors with Némethi and of Nakajima. As a special case, we compute generating series of Euler characteristics of non‐commutative Quot schemes of Kleinian orbifolds. In type A and rank 1, we give a second, combinatorial proof of our substitution formula, using torus localisation and partition enumeration. This gives a combinatorial model of the fibres of the variation of GIT map, and also leads to relations between our results and the representation theory of the affine and finite Lie algebras in type A.
Gromov–Witten invariants of bielliptic surfaces
Authors: Blomme Thomas 
Published: February 2025

AbstractBielliptic surfaces appear as a quotient of a product of two elliptic curves and were classified by Bagnera–Franchis. We give a concrete way of computing their GW‐invariants with point insertions using a floor diagram algorithm. Using the latter, we are able to prove the quasi‐modularity of their generating series by relating them to generating series of graphs for which we also prove quasi‐modularity results. We propose a refinement of these invariants by inserting a ‐class in the considered GW‐invariants.
On the real‐rootedness of the Eulerian transformation
Authors: Athanasiadis Christos A. 
Published: February 2025

AbstractThe Eulerian transformation is the linear operator on polynomials in one variable with real coefficients that maps the powers of this variable to the corresponding Eulerian polynomials. The derangement transformation is defined similarly. Brändén and Jochemko have conjectured that the Eulerian transforms of a class of polynomials with nonnegative coefficients, which includes those having all their roots in the interval , have only real zeros. This conjecture is proven in this paper. More general transformations are introduced in the combinatorial‐geometric context of uniform triangulations of simplicial complexes, where Eulerian and derangement transformations arise in the special case of barycentric subdivision, and are shown to have strong unimodality and gamma‐positivity properties. General real‐rootedness conjectures for these transformations, which unify various results and conjectures in the literature, are also proposed.
A stable splitting of factorisation homology of generalised surfaces
Authors: Kranhold Florian 
Published: February 2025

AbstractFor a manifold and an ‐algebra , the factorisation homology can be seen as a generalisation of the classical configuration space of labelled particles in . It carries an action by the diffeomorphism group , and for the generalised surfaces , we have stabilisation maps among the quotients which increase the genus . In the case where a highly‐connected tangential structure is taken into account, this article describes the stable homology of these quotients in terms of the iterated bar construction and a tangential Thom spectrum , and addresses the question of homological stability.
Flexible control of motor units: is the multidimensionality of motor unit manifolds a sufficient condition?
Authors: Dernoncourt François , Avrillon Simon , Logtens Tijn , Cattagni Thomas , Farina Dario , Hug François 
Published: February 2025

AbstractUnderstanding flexibility in the neural control of movement requires identifying the distribution of common inputs to the motor units. In this study, we identified large samples of motor units from two lower limb muscles: the vastus lateralis (VL; up to 60 motor units per participant) and the gastrocnemius medialis (GM; up to 67 motor units per participant). First, we applied a linear dimensionality reduction method to assess the dimensionality of the manifolds underlying the motor unit activity. We subsequently investigated the flexibility in motor unit control under two conditions: sinusoidal contractions with torque feedback, and online control with visual feedback on motor unit firing rates. Overall, we found that the activity of GM motor units was effectively captured by a single latent factor defining a unidimensional manifold, whereas the VL motor units were better represented by three latent factors defining a multidimensional manifold. Despite this difference in dimensionality, the recruitment of motor units in the two muscles exhibited similarly low levels of flexibility. Using a spiking network model, we tested the hypothesis that dimensionality derived from factorization does not solely represent descending cortical commands but is also influenced by spinal circuitry. We demonstrated that a heterogeneous distribution of inputs to motor units, or specific configurations of recurrent inhibitory circuits, could produce a multidimensional manifold. This study clarifies an important debated issue, demonstrating that while motor unit firings of a non‐compartmentalized muscle can lie in a multidimensional manifold, the CNS may still have limited capacity for flexible control of these units.
imageKey points
To generate movement, the CNS distributes both excitatory and inhibitory inputs to the motor units.
The level of flexibility in the neural control of these motor units remains a topic of debate with significant implications for identifying the smallest unit of movement control.
By combining experimental data and in silico models, we demonstrated that the activity of a large sample of motor units from a single muscle can be represented by a multidimensional linear manifold; however, these units show very limited flexibility in their recruitment.
The dimensionality of the linear manifold may not directly reflect the dimensionality of descending inputs but could instead relate to the organization of local spinal circuits.
Mitochondrial control of ciliary gene expression and structure in striatal neurons
Authors: Ulgen Dogukan H. , Chioino Alessandro , Zanoletti Olivia , Quintana Albert , Sanz Elisenda , Sandi Carmen 
Published: February 2025

AbstractMitochondria play essential metabolic roles and are increasingly understood to interact with other organelles, influencing cellular function and disease. Primary cilia, as sensory and signalling organelles, are crucial for neuronal communication and function. Emerging evidence suggests that mitochondria and primary cilia may interact to regulate cellular processes, as recently shown in brain cells such as astrocytes. Here, we investigated whether mitochondria also regulate primary cilia in neurons, focusing on molecular pathways linking both organelles and structural components within cilia. We employed a cross‐species, molecular pathway‐focused approach to explore connections between mitochondrial and ciliary pathways in neurons, revealing strong associations suggesting coordinated functionality. Furthermore, we found that viral‐induced downregulation of the mitochondrial fusion gene mitofusin 2 (Mfn2) in dopamine D1 receptor‐expressing medium spiny neurons (D1‐MSNs) of the nucleus accumbens (NAc) altered ciliary gene expression, with Crocc – the gene encoding rootletin – showing the most pronounced downregulation. This reduction in Crocc expression was linked to decreased levels of rootletin protein, a key structural component of the ciliary rootlet. Notably, viral‐mediated overexpression of rootletin restored ciliary complexity and elongation, without compromising neuronal adaptation to Mfn2 downregulation. Our findings provide novel evidence of a functional mitochondria–cilia interaction in neurons, specifically in striatal D1‐MSNs. These results reveal a previously unrecognized role of mitochondrial dynamics in regulating ciliary structure in neurons, with potential implications for neuropsychiatric and neurodegenerative disease mechanisms.
imageKey points
Mitochondria are cell structures known for producing energy but are also emerging as regulators of other cellular components, including primary cilia, antenna‐like structures involved in cell communication.
Previous studies suggest that mitochondria may influence cilia structure and function, including in astrocytes. However, this has not been explored in neurons.
This study shows that natural variation in mitochondrial molecular pathways correlates with primary cilia pathways in striatal medium spiny neurons in both rats and mice.
Reducing expression of mitofusin 2 (Mfn2), a key mitochondrial protein involved in fusion and mitochondria–endoplasmic reticulum interactions, changes specific molecular ciliary pathways, notably including Crocc, a gene essential for cilia structure, and reduces the levels of its protein product, rootletin, which supports cilia integrity.
Our findings reveal an important role for mitochondria in regulating ciliary structure in neurons, highlighting a potential pathway for mitochondrial regulation of neuronal signalling.
Dicarbonate Compounds as Electrolyte Solvents for Li-ion Cell Operation
Authors: Taskovic T. , Meisner Quinton , Azam Saad , Clarke Alison , Johnson Michel , Mistry Kevin , Carpenter Alex , Dahn J. R. 
Published: February 2025

Novel dicarbonate compounds were explored as a new class of electrolyte solvents for Li-ion batteries. Dimethyl-2,5-dioxahexane carboxylate (DMOHC) has been shown to be a high viscosity solvent that provides exceptional lifetime for Li-ion cells during elevated temperature cycling. Here, various dicarbonates were synthesized and screened for their physical properties, such as viscosity melting point and boiling point. Then, Li[Ni0.6Mn0.4Co0.0]O2/graphite (NMC640), Li[Ni0.83Mn0.06Co0.11]O2/graphite (Ni83), and LiFePO4/graphite (LFP) Li-ion pouch cells were used to evaluate the suitability of the various dicarbonates as electrolyte solvents for Li-ion cells. Cell testing was performed at 70 and 85 °C with various C-rate testing conditions. These novel dicarbonates were also blended with diethyl carbonate and dimethyl carbonate to lower viscosity and increase ionic conductivity. The findings suggest that several dicarbonate compounds can be viable electrolyte solvents for Li-ion cells operating only at elevated temperatures (70 °C+). It may not be possible to find a dicarbonate compound with low viscosity suitable as the main electrolyte solvent for Li-ion cells designed to operate at room temperature.
High Occurrence of a Missense Variant (c.471C>A) in the FGF23 Gene Related to Hyperostosis–Hyperphosphatemia Syndrome With a Possible Founder Effect
Authors: Sedghi Maryam , Gharehdaghi Elika Esmaeilzadeh , Ziaee Vahid , Abbasi Farzaneh , Meybodi Hamid Reza Aghaei , Smailey Elina , Mehdizadeh Mehrzad , Raeeskarami Seyyed Reza , Aslani Nahid , Shiran Sahar Naderi , Vafadar Mehdi , Amoli Mahsa M. 
Published: February 2025

Background: The autosomal recessive metabolic disorder hyperostosis–hyperphosphatemia syndrome (HHS) is characterized by hyperphosphatemia, hyperostosis, and recurrent bone lesions. Patients may develop ectopic and vascular calcification and may present diaphyseal pain of the long bones that is misdiagnosed as osteomyelitis. Mutations in GALNT3 and FGF23 genes were detected in patients with HHS. The main manifestations of these patients are increased levels of phosphate reabsorption from kidneys and painful swelling of long bones alongside with normal levels of vitamin D and parathormone.Method: We performed whole‐exome sequencing (WES) in seven Iranian patients. These patients were referred from several specialist clinics. Seven irrelevant families were examined for genetic mutations.Results: WES revealed the deleterious missense mutation c.471C>A, p. F157L in all affected members of six families. The variant c.1524+1G>A in the GALNT3 gene was found in the remaining patient which is reported previously. These variants were confirmed utilizing segregation studies in the pedigrees.Conclusion: Our data together with previous studies related to mutations in FGF23 in Iran strongly support that p. F157L mutation is abundantly prevalent in patients with Iranian origin and is likely to be a founder mutation; however, it requires further confirmative study. The result of this study suggests that p. F157L mutation should be investigated at the first step in genetic analysis of patients with HHS. This enables fast and accurate focused molecular diagnosis and would be effective for use in carrier screening as well as in prenatal diagnosis (PND) and preimplantation genetic diagnosis (PGD).
Transcriptomic gradients of the human cerebellum
Authors: King Leana , King Maedbh , Zhen Zonglei , Ivry Richard B. , Weiner Kevin S. 
Published: February 2025

Abstract
In the past decade, there has been major interest in understanding the role of transcriptomics in the functional and anatomical layout of the human brain. To date, almost all of the work linking transcriptomics to human brain function and structure has been restricted to the cerebral cortex. The culmination of this work has identified transcriptomics as an important shared principle that can tie together function, structure, and gene expression. However, largely missing from this work is the subcortex—namely the cerebellum. Here, we investigate whether transcriptomics offer a link between function and structure in the human cerebellum, using gene expression data from post-mortem cerebella and multi-modal brain atlases. We find that transcriptomic gradients from a sparse subset of genes align with a macroanatomical, rather than a functional - parcellation of the cerebellum, and the transition of the main gradient occurs at the horizontal fissure for the group, as well as individual cerebella. Conversely, when filtering for cortex-specific genes, there is an alignment with continuous functional gradients of the cerebellum, but not discrete parcellated areas.
Comparison of the transradial and transfemoral approaches for uterine artery embolization: a randomized clinical trial
Authors: Bessar Ahmed A , Libda Yasmin Ibrahim , Talaat Maichael , Tantawy Enjy Fathy , Heraiz Ahmed Ismail , Bessar Manar A 
Published: February 2025

Background Uterine artery embolization (UAE) is a procedure commonly used to control uterine bleeding or pain. While the procedure is traditionally performed through the transfemoral approach (TFA), the transradial approach (TRA) is another method. Purpose To compare the effectiveness of the UAE using the TRA approach versus the TFA approach. Material and Methods This non-blinded, randomized clinical trial was conducted at a tertiary hospital between 1 January 2019 and 30 June 2022. A total of 42 female patients with abnormal uterine bleeding and/or pelvic pain from uterine fibroids were randomly assigned to either the TRA group or the TFA group. Data collected included demographic information, procedural details, patient satisfaction, and radiation metrics. Results The TRA group had significantly lower numbers of microsphere vials used compared to the TFA group ( P = 0.014). While there were no significant differences in procedure times ( P = 0.058), fluoroscopic times ( P = 0.117), or radiation doses ( P = 0.466), the TRA approach was associated with a higher success rate in achieving bilateral UA catheterization and fewer instances of bilateral sheath insertion. Patient satisfaction scores were similar between the groups, with no statistically significant difference ( P = 0.932). Minor adverse events such as local hematoma and color changes were more frequent in the TFA group, though these differences were not statistically significant. Conclusion Although both approaches were effective for the UAE, the TRA approach may be a viable alternative to the TFA due to its higher success rate in achieving bilateral catheterization, lower radiation doses, and shorter procedural times.
Assessment of Cell Isolation From Human Milk Using Immunomagnetic Beads
Authors: Radhi Noor , Paul Ayamita , Muelbert Mariana , Toldi Gergely 
Published: February 2025

Background: There is increasing interest in better understanding the immune cell composition of human milk and how these cells interact with neonatal immune development. However, consistent methods for immune cell isolation from human milk are lacking. Research Aim: Our aim was to systematically compare available cell isolation techniques to isolate T lymphocytes from human milk samples. Methods: This repeated measures study design compared three cell isolation methods using human milk samples: (1) centrifugation, (2) immunomagnetic bead isolation, and (3) a combination of both methods. We assessed the proportion and viability of CD3+, CD4+, CD25+ and regulatory T cells using flow cytometry in isolated cells to compare the performance of these isolation methods. Results: Immunomagnetic separation is a feasible method to isolate T lymphocytes in human milk, similar to blood. It improves target cell enrichment and cell viability compared to centrifugation, which may be an advantage when the goal is to characterize rare cell types or when cells are further used in functional assays. No excess cell activation (CD25 positivity) was observed with the use of magnetic beads. Conclusion: Immunomagnetic separation of human milk T lymphocytes may have advantages over centrifugation depending on the intended downstream use of cells.
Thermal history dependent rheology, crystallization, and fusion joint strength of polyphenylene sulfide/carbon fiber thermoplastic composites
Authors: Ghanbari Lina N , Previte Joseph P , Wiggins Jeffrey S , McNair Olivia D 
Published: February 2025

The impact of thermal history on ultimate thermoplastic composite properties is often overlooked, provided the final parts are compliant. This work investigated the influence of temperature and time of common manufacturing processes of poly phenylene sulfide (PPS) thermoplastic composites (TPC), which resulted in long chain branching and crosslinking of the PPS matrix. This was determined through measurable changes such as rheological properties, crystallization behavior, and fusion joint strength of PPS/CF TPCs. TPC melt processing steps included unidirectional (UD) tape manufacturing, large panel layup and consolidation, individual panel thermal treatment, and fusion joining. During thermal treatment, nine sets of two 10″ × 4″ panels were exposed to either 300°C, 320°C, or 340°C, for 5 min, 30 min, or 60 min. Using thermal profiles identical to TPC manufacturing steps, rheological and calorimetric techniques mimicked the thermal history of the PPS/CF composites to observe changes in PPS matrix properties at discrete process steps. Complex viscosity of PPS increased across all test frequencies, while percent crystallinity decreased, indicative of thermal degradation in the melt state. The increase in viscosity inversely related to single lap shear strength (SLSS) of fusion joined panels. Through combining matrix-level measurements (SAOS rheology and DSC) to composite mechanical testing, we gain significant insight regarding the influence of process-induced polymer matrix properties on fusion joint strength.
Evolving Dynamics of Colorectal Cancer in High Socio-Demographic Regions
Authors: Konduru Laalithya , Dahia Simranjeet Singh , Szabo Claudia , Barreto Savio G. 
Published: February 2025

Background Colorectal cancer (CRC) poses a significant global health challenge, with evolving demographic trends emphasizing the need for accurate forecasting models. Existing forecasting models lack comprehensive coverage. By integrating machine learning algorithms, this study aims to provide more accurate and precise predictions, filling critical gaps in understanding CRC incidence, death, and disability-adjusted life year (DALY) rate trends, especially in high socio-demographic index (SDI) regions. Specific emphasis is placed on exploring age-, sex-, and country-specific variations in CRC trends. Materials and Methods An ensemble forecasting algorithm integrating Simple Linear Regression, Exponential Smoothing, and Autoregressive Integrated Moving Average, capable of handling a short time series was developed and validated, utilizing a dataset encompassing age-, sex-, and country-specific CRC incidence, mortality, and DALY rates. Results Our forecasting models reveal rising trends in CRC burden in the 15-49 years age group (young-onset) and decreasing trends in CRC burden in the 50-74 years age group (late-onset) in high SDI regions with sex-specific variations in incidence, mortality, and DALY rates. Some inflection points for demographic shifts in CRC disease burden, particularly death rates, were identified as early as within the next 5 years. We predict a shift in CRC burden towards females, particularly in older adults. Conclusion A novel multifactor model was developed for comparing the incidence, mortality, and DALY rates of young- and late-onset CRC in high SDI regions. The rising incidence of young-onset CRC in high SDI regions underscores the need for proactive health strategies. By refining predictive models, adjusting screening guidelines to target younger, high-risk populations, and investing in public awareness and research, we can facilitate early detection and improve outcomes. This study addresses a significant gap in CRC forecasting and provides a robust framework for anticipating demographic shifts in CRC burden, making it an indispensable tool for healthcare planning.
Practical use of nadofaragene firadenovec-vncg for pharmacists
Authors: Holler Seth D , Smith Sara Moran 
Published: February 2025

Objective To provide a practical approach to pharmacists regarding the safe and effective use of nadofaragene firadenovec. Data sources Clinical study data and pharmacist experience in institutional settings. Data summary This review article discusses the role of pharmacists caring for patients with Bacillus Calmette-Guérin (BCG)–unresponsive non–muscle-invasive bladder cancer (NMIBC) receiving nadofaragene firadenovec. Pharmacist roles in a multidisciplinary care team are described and involve considerations surrounding nadofaragene firadenovec, including its mechanism of action, indication, handling, storage, administration, adverse event profile, disposal, counseling, and other practical points. Conclusions Pharmacists are positioned to be key contributors to the care team regarding the effective use of nadofaragene firadenovec. Further awareness and education will continue to optimize the clinical use of nadofaragene firadenovec in the high-risk BCG-unresponsive NMIBC setting.