bluuebunny/crossref_metadata_2025_split huggingface dataset:
Apoptin Mediates Endogenous Mitophagy and Apoptosis by Regulating the Level of ROS in Hepatocellular Carcinoma
Authors: Li Yiquan , Shang Chao , Liu Zirui , Han Jicheng , Li Wenjie , Xiao Pengpeng , Li Nan , Li Shanzhi , Xiu Zhiru , Song Gaojie , Li Yaru , Jin Ningyi , Fang Jinbo , Li Xiao , Zhu Yilong 
Published: December 2021

Abstract
Background: Apoptin, as a tumor-specific pro-apoptotic protein, apoptin plays an important role in the field of anti-tumor, but its autophagy activation mechanism and the interaction between autophagy and apoptosis have not been accurately elucidated. Here, we studied the mechanism of apoptosis and autophagy induced by apoptin and the interaction between autophagy and apoptosis. Methods: Through crystal violet staining and CCK-8 assay, we analyzed the effect of apoptin in inhibiting liver cancer in vitro, and also analyzed the effect of inhibiting liver cancer in vivo by establishing a nude mouse tumor model. Flow cytometry and fluorescence staining were used to analyze the main types of apoptosis and autophagy induced by apoptin. Subsequently, the relationship between apoptosis and autophagy induced by apoptin was analyzed. Then, flow cytometry was used to analyze the effect of ROS on apoptosis and autophagy mediated by apoptin. Then, the affect of ROS on apoptosis and autophagy mediated by apoptin was analyzed. Finally, the key genes leading to autophagy were analyzed by silencing different genes.Results: The results showed that apoptin can significantly increase the apoptosis and autophagy of liver cancer cells, and apoptin can cause mitophagy through the increase of NIX protein. Apoptin can also significantly reduce the level of cellular ROS, which is related to the autophagy and apoptosis of liver cancer cells caused by apoptin. The change of ROS may be a key factor causing apoptosis and autophagy. Conclusion: The above results indicate that the increase of ROS level after apoptin treatment of liver cancer cells leads to the loss of mitochondrial transmembrane potential, which leads to endogenous apoptosis and mitophagy while recruiting NIX. Therefore, ROS may be a key factor connecting endogenous apoptosis and mitophagy induced by apoptin in liver cancer cells.
Allied Health and Complementary Therapy Usage in Australian Women With Chronic Pelvic Pain: a Cross-Sectional Study
Authors: Malik Astha , SINCLAIR Justin , Ng Cecilia , Smith Caroline , ABBOTT Jason , ARMOUR Mike 
Published: December 2021

Abstract
Background Chronic pelvic pain (CPP) causes non-cyclical pelvic pain, period pain, fatigue and other painful symptoms. Current medical and surgical management strategies are often not sufficient to manage these symptoms and may lead to uptake of other therapies. Aims To determine the prevalence of allied health (AH) and complementary therapy (CM) use, the cost burden of these therapies and explore predictive factors for using allied health or complementary medicines.Materials and Methods An online cross-sectional questionnaire using the WERF EndoCost tool was undertaken between February to April 2017. People were eligible to participate in the survey if they were aged 18-45, living in Australia and had chronic pelvic pain. Results From 409 responses, 340/409 (83%) of respondents reported a diagnosis of endometriosis. One hundred and five (30%) women with self-reported endometriosis, and thirteen (18%) women with other forms of CPP saw at least one AH or CM practitioner in the previous two months, with physiotherapists and acupuncturists the most common. Women who accessed CM or AH services spent an average of $480.32 AUD in the previous two months. A positive correlation was found between education and number of AH or CM therapies accessed in the past two months (p<0.001) and between income level and number of therapists (p=0.028).Conclusions Women with CPP commonly access AH and CM therapies, with a high out of pocket cost. The high cost and associations with income and education levels may warrant a change to policy to improve equitable access to these services.
Effects of the Experience of Breastfeeding-Friendly Practices and Breastfeeding Intention and Self-Efficacy on Breastfeeding Behavior: A Cohort Study in Taiwan
Authors: Wang Yu-Wen , Chang Ying-Ju 
Published: December 2021

Abstract
Background: Approximately 80% of births occurred in Baby-Friendly-accredited facilities in Taiwan, although the trend of exclusively breastfeeding infants until 6 months of age has stagnated in the last 10 years. To guide breastfeeding promotion interventions during postnatal stays and encourage mothers to continue breastfeeding for the first 6 months, the factors of breastfeeding behavior within 6 months post-delivery must be investigated. This study explored the relationships among breastfeeding intention, experience of breastfeeding-friendly practices, breastfeeding self-efficacy, and breastfeeding behavior at 4 and 6 months after childbirth.Methods: In this longitudinal cohort study, women who gave birth to healthy newborns at two Baby-Friendly hospitals in Taiwan and who were willing to breastfeed were recruited. Data were collected through a self-developed questionnaire measuring the experience of breastfeeding-friendly practices and through the Chinese version of the Breastfeeding Self-Efficacy Scale-Short Form during hospitalization and at 1, 2, 4, and 6 months after childbirth.Results: A total of 155 women completed the questionnaires 5 times within 6 months. The determinants of exclusive breastfeeding at 4 months and any breastfeeding at 6 months were longstanding breastfeeding intention during hospitalization, a high score for the experience of breastfeeding-friendly practices, and a high level of breastfeeding self-efficacy during that period. The in-hospital experience of breastfeeding-friendly practices did not predict breastfeeding behavior at 4 and 6 months. Breastfeeding intention (hazard ratio [HR] = 0.40–0.14) and in-hospital breastfeeding self-efficacy (HR = 0.98) were significant as in-hospital risk factors contributing to breastfeeding duration 6 months after childbirth.Conclusions: Longstanding breastfeeding intention, continuation of the experience of breastfeeding-friendly practices, and maintenance of a high level of breastfeeding self-efficacy were the determinants of breastfeeding at 4 and 6 months. Healthcare professionals in Taiwan must support breastfeeding-friendly practices and consider interactive interventions to promote continued breastfeeding at different stages during the first 6 months after childbirth based on the mother’s informed plan of breastfeeding and breastfeeding self-efficacy during their postnatal hospitalization.
Phenotypical Comparison of Pseudomonas Aeruginosa Isolated from Human and Veterinary Samples; Impact of Host Adaptation on Infection Pathogenesis
Authors: Kaiser Stefan J. , DeRosa Annalisa , Ewers Christa , Günther Frank 
Published: December 2021

Abstract
Purpose: Determinants of virulence in Pseudomonas aeruginosa vary strongly depending on its habitat. In this study, we analyzed these alterations depending on the host organism in isolates cultured from canine ears and compared it to clinical extended-spectrum antibiotic-resistant Pseudomonas aeruginosa isolates (XDR), clinical antibiotic-sensitive (non-XDR) from humans and environmental isolates (EI) analyzed during our first study in 2017. Methods: A total of 22 veterinary isolates cultured from canine ears (VET) were examined for spontaneous biofilm formation, stress response in biofilm formation induced by meropenem, in vitro fitness, susceptibility to human serum and polymorphonuclear leukocytes and the genetically determined virulence factors toxA, exoS, exoT, exoU, exoY, nan1, cif, lasA and lasB.Results: We observed significantly elevated spontaneous biofilm formation and serum susceptibility in VET isolates compared to EI and non-XDR isolates as well as significantly decreased in vitro fitness compared to XDR isolates. The VET isolates resembled most the XDR subgroup of isolates previously cultured from blood. Within the environmental isolates, we observed an increase of spontaneous biofilm formation and exoU presence in isolates cultured from community water samples over hospital water samples to pool samples.Conclusions: Considering the distinct differences in some features of the examined VET isolates, a higher degree of phenotypical adaption can be assumed. Increased biofilm formation seems to be a common and characteristic event in isolates adapted to a specific habitat. Therefore amplification of potentially more virulent Pseudomonas aeruginosa strains in domestic animals may lead to elevated zoonotic risk for example for pet owners.
Isolation, Marginalisation and Disempowerment – Understanding How Interactions with Health Providers Can Influence Smoking Cessation in Pregnancy
Authors: Fletcher Cherise , Hoon Elizabeth , Gialamas Angela , Dekker Gustaaf , Lynch John , Smithers Lisa 
Published: December 2021

Abstract
BackgroundMaternal smoking during pregnancy can lead to serious adverse health outcomes for both women and their infants. While smoking in pregnancy has declined over time, it remains consistently higher in women with lower socioeconomic circumstances. Furthermore, fewer women in this group will successfully quit during pregnancy. AimThis study explores the barriers to smoking cessation experienced by socially disadvantaged pregnant women and investigates how interactions with health providers can influence their smoking cessation journey.Methodsomen (either pregnant or birthed in the previous 10 years, who smoked or quit smoking in pregnancy) were recruited from a metropolitan public hospital antenatal clinic in South Australia and community organisations in surrounding suburbs. Seventeen women participated in qualitative semi-structured small focus groups or interviews. The focus groups and interviews were recorded, transcribed and thematically analysed. FindingsFour interconnected themes were identified: 1) smoking embedded in women’s challenging lives and pregnancies, 2) cyclic isolation and marginalisation, 3) feeling disempowered, and 4) autonomy and self-determination. Themes 3 and 4 are characterised as being two sides of a single coin in that they coexist simultaneously and are inseparable. A key finding is a strong unanimous desire for smoking cessation in pregnancy but women felt they did not have the necessary support from health providers or confidence and self-efficacy to be successful.ConclusionWomen would like improvements to antenatal care that increase health practitioners’ understanding of the social and contextual healthcare barriers faced by women who smoke in pregnancy. They seek assistance from health providers to make informed choices about smoking cessation and would like women-centred care. Women feel that with greater support, consistency and encouragement from health providers they could be more successful at antenatal smoking cessation.
A Descriptive Study to Assess Wellbeing, Quality of Life and Satisfaction of Omani Patients with Chronic Wounds
Authors: Natarajan Jansi Rani , Joseph Mickael Antoine , Alawi Rashid Mohammed Al , Balushi Taimoor Al , Alawi Ibrahim Al , Junaibi Suad Moosa Al , John Anitha Nesa Thanka , Balushi Laila bint Darwish Al , Ismaili Issa Sulaiman Al , Shummo Moath 
Published: December 2021

Abstract
Background: A chronic wound is one that has failed to proceed through the normal phases of wound healing in an orderly manner. Chronic wounds are a common problem around the world. They can be distressing to the patients causing physical, social and emotional damage such as decreased activities of daily living, pain, discomfort, unpleasant odor, and insomnia. Wellbeing, quality of life and satisfaction of these patients with chronic wounds can be impaired by a complex set of aspects. The purpose of this article is to describe the impact of chronic wounds on the wellbeing, quality of life and satisfaction of Omani patients with chronic wounds. Methods: This is a descriptive cross-sectional study of 275 patients with chronic wounds attending three tertiary care hospitals in Muscat city, Sultanate of Oman. Adult patients of 18 years and above with wounds of duration of more than 4weeks were chosen by consecutive non-probability sampling. A pre-tested, semi-structured self-reported questionnaire was provided to each patient to complete. Data was analysed using SPSS IBM version 23.Results: The average age of the patients was 47.13±16.917 and the majority of them (64.7%) were males. Most of them (29.1%) had diabetic ulcers and 45.8% of them presented with mildly severe wounds based on Bates-Jensen wound assessment scale. Patients with chronic wounds reported a poor wellbeing scores (M=10.47, SD= 1.813), moderate quality of life (M=5.22, SD= 2.388) and satisfaction scores (M=6.89, SD= 2.388). Significant mean differences were reported with age, gender, educational level, and type of wound at p<.05 levels.Conclusion: These findings demonstrated that chronic wounds could influence the wellbeing, quality of life and overall satisfaction with the quality of life of patients suffering with it. These results also suggest a need to pay increased attention by the healthcare providers to the patients suffering from chronic wounds on the aspects of wellbeing and quality of life along with treating the physical symptoms.
Thromboembolic Events in Critically Ill Children with Multiple Risk Factors for Venous Thrombosis
Authors: Östlund Åsa KM , Fläring Urban , Larsson Peter , Kaiser Sylvie , Vermin Lena , Frisk Tony , Dahlberg Ann , Berner Jonas , Norberg Åke , Andersson Andreas 
Published: December 2021

Abstract
The risk for venous thromboembolism (VTE) is considered to be low in the general paediatric intensive care unit (PICU) population, and pharmacological thromboprophylaxis is not routinely used. PICU patients considered at high-risk of VTE could possibly benefit from pharmacological thromboprophylaxis, but the incidence of VTE in this group of patients is unclear. This was an observational, prospective study at a tertiary multi-disciplinary paediatric hospital. We used comprehensive ultrasonography screening for VTE in critically ill children with multiple risk factors for VTE. Patients admitted to PICU ≥72 hours and with ≥two risk factors for VTE were included. Patients receiving pharmacological thromboprophylaxis during their entire PICU stay were excluded. Since pharmacological thromboprophylaxis has not been proven effective for central venous catheter(CVC)-related VTE, the primary outcome was VTEs not related to the use of a CVC. Ultrasonography screening of the great veins was performed at PICU discharge. Seventy patients with median (interquartile range) 3 (2-4) risk factors for VTE were evaluated. Median age was 0.34 years (0.03-4.3) and median PICU length of stay 9 days (5-17). Regarding the primary outcome, no symptomatic VTEs occurred and no asymptomatic VTEs were found on ultrasonography screening. The observed proportion of VTE, 0 in 70 patients, corresponds to a VTE incidence of 0-5.1%. Conclusion: No VTEs where pharmacological thromboprophylaxis could potentially have been an effective prophylactic measure were found. This indicates that VTE is an uncommon event even in a selected group of severely ill small children considered to be at high risk of VTE.
The Symptom Discounting Effect : What to Do When Negative Genetic Test Results Become Risk Factors for Alcohol Use Disorder
Authors: ahn woo-kyoung , Perricone Annalise 
Published: December 2021

Abstract
Most consumers of genetic testing for health conditions test negative, yet the psychological perils of this are hardly known. In three experiments (N=2,103) participants discounted repercussions of Alcohol Use Disorder (AUD), after learning or imagining that they were not genetically predisposed to AUD. Such discounting can lead people to avoid treatment and to feel safe to continue or even increase their drinking, ironically turning the negative genetic feedback into a risk factor for AUD. This misconception derives from not understanding the Causal Markov condition as applied to this case; once AUD symptoms are present, their ramifications remain the same regardless of whether genes or environments caused the symptoms. Educating participants about this principle mitigated the irrational discounting of threats of AUD, even among Individuals already engaging in problematic drinking, for whom the debriefing currently used by a direct-to-consumer genetic testing company was found to be ineffective in the current study.
A Systematic Review and Meta-analysis of Effect of Probiotic Supplementation on Age-related Sarcopenia in Elderly Adults
Authors: Saraf-Bank Sahar , Navab Fatemeh , Rouhan Mohammad Hossein , Hajhashemy Zahra , Shokri-mashhadi Nafiseh 
Published: December 2021

Abstract
Background: Several clinical studies emphasized the role of bio-diversities of gut microbiota in age-related disorders. Nevertheless, the effect of probiotic administration on sarcopenia indices are unclear. This meta-analysis aimed to investigate the effect of probiotic administration on muscle strength, muscle mass, and muscle function. Methods: We assessed all interventional studies through different electronic databases including PubMed, Scopus, ISI –Web of Science, and Cochrane library using defined keywords from inception to Jun 2021. Studies that investigated the effect of probiotic administration on at least one of the components of sarcopenia or anthropometric indices versus non- probiotics in old adults (>55 years) were included. Results: The initially overall effect of meta-analysis on 1393 participants declared a null effect of probiotic supplementation on main outcomes, including muscle mass (WMD: -0.05, 95% CI: -1.54, 1.43; I-square: 0.0%, P=0.985), and muscle strength (WMD: 0.7, 95% CI: -0.01, 1.40; I-square: 76.8%, P=0.005). Subgroup analysis showed that administration of probiotic supplementation for more than 12 weeks significantly increased muscle strength (WMD: 1.15, 95% CI: 0.86, 1.43; I-square: 0.0%, P=0.679). However, probiotic supplementation had no effect on anthropometric indices, including body mass and body mass index, (WMD: -0.05, 95% CI:-2.57, 1.56; I-square: 0.0%, P=0.976) and (WMD: 0.08, 95% CI:-0.16, 0.32; I-square: 0.0%, P=0.718), respectively. Conclusion: This study confirmed the positive impact of probiotic supplementation on the muscle strength (based on the last definitions by EWGSOP), in particular, probiotic administration for more than 12 weeks. More clinical trials on sarcopenic elderly subjects are wanted to confirm our findings.
Piezoelectric Voltage Constant and Sensitivity Enhancements Through Phase Boundary Structure Control of Lead-free (K,Na)NbO3-based ceramics
Authors: Lee Min-Ku , Kim Byung-Hoon , Lee Gyoung-Ja 
Published: December 2021

Abstract
The piezoelectric voltage constant (g33) is a material parameter critical to piezoelectric voltage-type sensors for detecting vibrations or strains. Here, we report a lead-free (K,Na)NbO3 (KNN)-based piezoelectric accelerometer with voltage sensitivity enhanced by taking advantage of a high g33. To achieve a high g33, the magnitudes of piezoelectric charge constant d33 and dielectric permittivity er of KNN were best coupled by manipulating the intrinsic polymorphic phase boundaries effectively with the help of Bi-based perovskite oxide additives. For the KNN composition that derives benefit from the combination of er and d33, the value of g33 was found to be 46.9 ´ 10-3 V·m/N, which is significantly higher than those (20 - 30 ´ 10−3 V·m/N) found in well-known polycrystalline lead-based ceramics including commercial Pb(Zr,Ti)O3 (PZT). Finally, the accelerometer sensor prototype built using the modified KNN composition demonstrated higher voltage sensitivity (183 mV/g) when measuring vibrations, showing a 29% increase against the PZT-based sensor (142 mV/g).
I-V Characteristic and Performance of Three Electrode Alkaline Electrolyzer
Authors: Kafle Diwakar , Dumre Sushil , Tripathi Saroj , Shrestha Shankar 
Published: December 2021

Abstract
Hydrogen production by electrolysis of water is seen as a promising technique as it is environment friendly and it can use renewable energy source for the production of hydrogen gas. However, this technology has less than 4% contribution to the production of commercial hydrogen in the market. This is due to the high electricity consumption of the water splitting reaction. The main challenge to make this technology efficient and economically viable is to develop cost effective and highly efficient electrolyzer. Here we have developed a three electrode electrolyzer in which an extra electrode is inserted between conventional electrodes: cathode and anode. This novel electrolyzer utilizes an extra voltage source which reduces the overpotential and increases the anode current of the cell, which is responsible for the hydrogen production. Furthermore, we observed that, the operating resistance of the cell decreases under the application of the new voltage source. Our results demonstrate that the introduction of third electrode improves the performance of electrolysis by consuming less power as compared to the traditional or conventional two electrode electrolyzer system.
S-palmitoylation and sterol interactions mediate antiviral specificity of IFITM isoforms
Authors: Das Tandrila , Yang Xinglin , Lee Hwayoung , Garst Emma , Valencia Estefania , Chandran Kartik , Im Wonpil , Hang Howard 
Published: December 2021

Abstract
Interferon-induced transmembrane proteins (IFITM1, 2 and 3) are important antiviral proteins that are active against many viruses, including influenza A virus (IAV), dengue virus (DENV), Ebola virus (EBOV), Zika virus (ZIKV) and severe acute respiratory syndrome coronavirus (SARS-CoV). IFITMs exhibit isoform-specific activity, but their distinct mechanisms of action and regulation are unclear. Since S-palmitoylation and cholesterol homeostasis are crucial for viral infections, we investigated IFITM interactions with cholesterol by molecular dynamic stimulations, nuclear magnetic resonance analysis in vitro and photoaffinity crosslinking in mammalian cells. These studies suggest that cholesterol can alter the conformation of IFITMs in membrane bilayers and directly interact with S-palmitoylated IFITMs in cells. Notably, we discovered that the S-palmitoylation levels regulate differential IFITM isoform interactions with cholesterol in mammalian cells and specificity of antiviral activity towards IAV, SARS-CoV-2 and EBOV. Our studies suggest that modulation of IFITM S-palmitoylation levels and cholesterol interaction may influence host susceptibility to different viruses.
Experimental observation of permanent magnet rotation
Authors: Tong Weiming , Chen Bihe 
Published: December 2021

Abstract
Why does the Earth rotate? At present, several theories on Earth rotation remain hypotheses. Hence, the aim of this study was to obtain experimental evidence of the relationship between the rotational force and magnetic field so that we can use experimental devices to demonstrate the rotation relationship among the planets and the sun. Each permanent magnet rotating under the action of an external force is installed on the shaft of DC motor; each magnetic ball designed to rotate in a magnetic field is placed in the center of a hollow sphere that can float on the water. Using the above setup, the experimental methods and procedures based on this research can be used to observe the rotation behaviour of a permanent magnet in a magnetic field, understand the reason for its rotation, and determine the strength of the rotational force of the permanent magnet in the magnetic field.