bluuebunny/crossref_metadata_2025_split huggingface dataset:
An Imbalance of IL-33/ST2-AXL-Efferocytosis Axis Induces Pregnancy Loss Through Metabolic Reprogramming of Decidual Macrophage
Authors: Sheng Yan-Ran , Hu Wen-Ting , Shen Hui-Hui , Wei Chun-Yan , Liu Yu-Kai , Ma Xiao-Qian , Li Ming-Qing , Zhu Xiao-Yong 
Published: November 2021

Abstract
During the implantation of embryo, apoptosis is inevitable. These apoptotic cells (AC) are removed by efferocytosis, which fills the macrophage with a metabolite load nearly equal to the phagocyte itself. A timely question pertains to the interrelationship between efferocytosis metabolism and the immune behavior of decidual macrophages (dMΦs) and its effect on pregnancy outcome. Here we report a positive feedback of IL-33/ST2-AXL-efferocytosis leading to pregnancy failure through metabolic reprogramming of dMΦs. We compared the serum level of IL-33, sST2, along with IL-33, ST2, efferocytosis and metabolism of dMΦs from both normal gravidas and unexplained recurrent pregnancy loss (RPL) patients. And we revealed the disturbance of IL-33/ST2 axis, increased apoptotic cells and elevated efferocytosis of dMΦs from the patients with RPL. Afterwards the dMΦs swallowing so many apoptotic cells secreted more sST2 and less TGF-β, which polarized dMΦs towards M1 phenotype. Moreover, the elevated sST2 biased the efferocytosis metabolism of RPL dMΦs towards oxidative phosphorylation and exacerbated the disruption of IL-33/ST2 signaling pathway. The metabolic disorders also led to the dysfunction of efferocytosis, resulting in more uncleared apoptotic cells and the secondary necrosis occurred. We also screened efferocytotic molecule AXL regulated by IL-33/ST2. This positive feedback of IL-33/ST2-AXL-efferocytosis led to pregnancy failure. And the IL-33 knockout mice demonstrated poor pregnancy outcomes, and exogenous supplementation of mouse IL-33 could partially alleviate the fate of embryo losses. These findings highlight a new etiological mechanism whereby dMΦs leverage immunometabolism for the homeostasis of microenvironment at the maternal-fetal interface.
Sensitivity of Some Sweet Cherry (Prunus Avium L.) Genotypes To Late Spring Frosts During Different Phenological Stages Following Bud Burst
Authors: Kaya Ozkan , KOSE Cafer 
Published: November 2021

Abstract
Sweet cherry (Prunus avium L.) is one of the economically important fruit crops worldwide. However, late spring frosts occurring in some years can significantly impact sweet cherry productivity through organ and tissue destruction caused by frost damage, and very little is known about frost tolerance or susceptibility of new cultivars. Differential thermal analysis (DTA) was, therefore, used to examine the exothermic characteristics (temperatures at which 50% of the flower buds were killed - mLTE values) of the flower buds belonging to members of the genus Prunus - 6 Prunus avium cultivars ('Noir de Guben', 'Bigarreau Gaucher', 'Merton Late', 'Merton Bigarreau', 'Van' and wild genotype). In the study, mLTE values of flowers of six cherry cultivars were determined at different floral bud developmental stages such as side green, green tip, open cluster, first white and full bloom under laboratory-based freeze assays for consecutive two years. The mLTE values of flower buds changed according to both different floral bud developmental stages and sweet cherry cultivars. In our findings, the mLTE values of flower buds in all cultivars generally occurred at higher temperatures in the open cluster stage, whereas the mLTE values of the flower buds in the first white stage occurred at lower temperatures, and therefore, these results are not only controversial in terms of previous frost tolerance studies, but also the first findings to be reported in literature. Considering the two-year average, the temperatures causing mLTE values for flower buds was -1.58 to -3.74°C at the side green stage, -0.94 to -3.51°C at the green tip stage, -0.41 to -1.96°C at the open cluster stage, -2.30 to -11.52°C at the first white stage and -2.37 to -9.80°C at the full bloom stage in the range of six cultivars. In laboratory-based freezing experiments, the 'Van' cultivar were least affected by low temperatures, followed by 'Bigarreau Gaucher' cultivar and wild genotype. 'Merton Late' cultivar, on the contrary, was the most sensitive cultivar to low temperatures, followed by 'Noir de Guben' and 'Merton Bigarreau'. These results can be valuable in predicting possible frost damage at different developmental stages of the flower buds in sweet cherry.
Hepatitis B Virus Associated Hepatocarcinoma Activated Unique Metabolism Pathway to Gain Survival Advantages
Authors: Zhao Yingke , Ning Zhouyu , Deng Yong , Li Bing , Sun Yun , Meng Zhiqiang 
Published: November 2021

Abstract
Background: Hepatitis B Virus (HBV) is considered as a “metabolic virus” and affects the hepatic metabolism homeostasis, while except for the anti-virus therapy the principle of HBV-negtive hepatocellular carcinoma (HCC) and HBV-positive HCC is exactly the same until now. Exploration of the unique characteristics of HBV-associated HCC will provide new metabolic targets against HBV-associated HCC to gain survial advantage, and finally inhibit cancer progression. Methods: To systematically assess the metabolic changes, we assessed the transcriptomic and proteomic data from public database, additionally verified the accordingly changes in our own test cohort on proteome and transcriptome level. Additionally, the tissue metabolomics profile, lipidomics and the activity of PI3K/AKT/mTOR signaling pathway which play critical role in metabolism reprogramming, were analyzed to confirm the aforementioned changes. Results: By multi-omics approaches, we presented the metabolic dysfunction experienced during HBV-associated HCC, represented by upregulated the steroid hormone biosynthesis, primary bile acid, and sphingolipid metabolism which related to a poor prognosis. Metabolites in linoleic acid metabolism and primary bile acid metabolism were closely correlated with HBV replication in HBV-positive HCC patients. Further tissue metabolomics and lipidomics validate the serum metabolic changes, namely changing in lipid composition and unsaturated fatty acid accumulation. By antibody array, a hyperactive PI3K/AKT/mTOR signaling transduction in HBV-positive biopsy compared with HBV-negative patients was observed, indicating a key role of this pathway in metabolic reprogramming of HBV-associate HCC. Conclusion: HBV plays a critical role in the metabolism of HBV-associated HCC and activated a unique metabolism pathway to gain survival advantages. HBV-positive HCC showed obviously difference compared to HBV-negative HCC in metabolism at least and should be treated according to its characteristics in the clinic.
Do Gut Microbiota Correlate with Stereotypic Behavior? First Answers from Malayan Sun Bears and Insights into Ex-situ Conservation
Authors: Wu Hong , Wu Nan , Yao Hongyu , Guo Xinyu , Zhang Sheng , Wang Yuan , Zhou Qihai , Zhao Dapeng 
Published: November 2021

Abstract
Gut microbiota affect host health and behavior throughout life but their relationship in endangered animals is poorly understood. Malayan sun bears are protected as an endangered wild species. Abnormal behavior such as stereotypies can be used as welfare indicators of animals in zoos. In this study, the abundance and diversity of fecal microbiota from six Malayan sun bears in Tianjin Zoo were assessed using 16S rRNA Illumina technology, and stereotypies were analyzed to identify behavioral patterns in the bears, separated into two age groups. Stereotypic behavior did not differ significantly between bears of different ages, but bacterial diversity in bears from the elderly group was significantly higher than that of the adult group. The gut microbiota of Malayan sun bears was dominated by Firmicutes (54.70%), Proteobacteria (41.90%), and Fusobacteria (1.80%). Findings from this study facilitate understanding of the relationship between gut microbiota and behavioral patterns, which could benefit the welfare of animals in captivity.
New Optical Soliton Solutions For The Variable Coefficients Nonlinear Schrodinger Equation
Authors: Gu Yongyi , Aminakbari Najva 
Published: November 2021

Abstract
This paper is appropriated to seek new optical soliton solutions of nonlinear Schrodinger equation (NLSE) with time-dependent coefficients which describes the dispersion decreasing fiber. By proposing Bernoulli (G ′/G)-expansion method, where G = G(ζ ) satisfies Bernoulli equation, some periodic wave, bright and dark soliton solutions are successfully achieved. In addition, 3D, line and contour maps graphs of the obtained results under effect of different values of coefficients are illustrated to have acceptable conception of dynamic structures.
ctDNA as A Biomarker of Progression in Oesophageal Adenocarcinoma
Authors: Bonazzi Vanessa F. , Aoude Lauren G. , Brosda Sandra , Lonie James M. , Patel Kalpana , Bradford Julia J. , Koufariotis Lambros T. , Wood Scott , Smithers B. Mark , Waddell Nicola , Barbour Andrew P. 
Published: November 2021

Abstract
Background: The incidence of oesophageal adenocarcinoma (OAC) is rapidly increasing and despite improvements in treatment, the five-year survival rate remains poor. Prognostic biomarkers that address the genomic heterogeneity in this highly complex disease will aid the development of precision therapeutics and improve patient survival. The aim of this study was to determine whether circulating tumour DNA (ctDNA) has prognostic significance as a biomarker in OAC patients.Methods: We profiled 209 blood and tumour samples from 57 OAC patients. Using a panel of 77 cancer genes, we sequenced ctDNA in plasma samples (n=127) which were taken at multiple time points pre- and post-therapy. In parallel, we sequenced matched tumour samples from 39 patients using the same gene panel. To assess whether the ctDNA profile reflected the tumour heterogeneity, we sequenced additional multi-region primary tumour samples (n=17). In addition, we analysed whole-genome and whole-exome sequencing data from primary tumours for a subset of 18 patients. Results: Using a tumour agnostic approach, we found that detectable ctDNA variants in post-treatment plasma samples were associated with worse disease specific survival. To evaluate whether the ctDNA originated from the primary tumour, we performed a tumour informed analysis which confirmed post-treatment ctDNA variants were associated with worse survival. To determine whether ctDNA could be used as a clinical follow-up test, we assessed blood samples from multiple time points before and after treatment, in a subset of patients. Results showed that the variant allele frequency of ctDNA variants increased with disease recurrence. Conclusion: This study demonstrates that ctDNA variants can be detected in patients with OAC and this has potential clinical utility as a prognostic biomarker for survival.
Trends of the Incidence and Prevalence of Metabolic Syndrome Among Participants of the Specific Health Check and Guidance System in Japan
Authors: Iseki Kunitoshi , KONTA TSUNEO , ASAHI KOICHI , FUJIMOTO SHOUICHI , MORIYAMA TOSHIKI , YAMAGATA KUNIHIRO , TSURUYA KAZUHIKO , NARITA ICHIEI , KONDO MASAHIDE , KASAHARA MASATO , SHIBAGAKI YUGO , ISEKI CHIHO , WATANABE TSUYOSHI 
Published: November 2021

Abstract
A specific nationwide health check-up and guidance system (Tokutei-Kenshin) was initiated in April 2008 to detect metabolic syndrome (MetS) and if confirmed, to provide individual instruction to modify lifestyle and the necessary treatment. However, trends of the incidence and prevalence of MetS are not yet available. Data of the Tokutei-Kensin from 2008 to 2014 were used in this study. The total number of participants was 3,809,853. Among them, we identified 933,490 individuals who have screened at least twice during the study period. The mean number of visits was 3.4 times per person. Incidence of MetS was defined as those who were MetS (-) at the first screening and developed MetS next year. Persistent prevalence of MetS was defined as those who were MetS (+) at both the first and next year screening. We obtained five 1-year incidence and persistent prevalence of MetS during the study period. The average 1-year incidence of MetS was 5.7% (6.6%, 2.7%) and the average prevalence of MetS was 10.1% (16.1%, 6.2%) in the total (men, women) participants, respectively. The average persistent prevalence of MetS was 47.3% (49.6%, 43.3%) in the total (men, women) participants, respectively. Both incidence and prevalence of MetS were higher in men than that in women. Compared to those of age less than 65 years old, elderly participants had a higher incidence and prevalence of MetS. During the study period of 2008 to 2014, there were no apparent changes in the incidence, prevalence, and persistent prevalence of MetS.
Dimethyl Fumarate Abrogates Growth and Metastasis of Hepatocellular Carcinoma by Regulating NRF2/Bcl-XL Axis and Synergizes with Sorafenib
Authors: Faleti Oluwasijibomi Damola , Zhang Mingjiao , Tan Haiqi , Gong Yibing , Zhang Yuanbin , Deng Simin , Liu Pengfei , Huang Jing , Wu Gongfa , Wang Xiong , Chen Ying , He Mingliang , Lyu Xiaoming 
Published: November 2021

Abstract
BackgroundEmerging evidence indicates that the mitochondria play an important role in poor treatment outcomes observed in liver malignancies. Hepatocellular carcinoma (HCC) remains a leading cause of cancer mortality worldwide, and there is still no effective chemotherapeutic treatment. Dimethyl fumarate (DMF), a derivative of fumaric acid, is used to treat psoriasis and multiple sclerosis. MethodsHCC mice model was used in this study. Eight-week-old mice bearing tumor were administered either DMF (30 mg/kg/day; n = 6) or DMSO (control group; n = 6) for 2 weeks. RNA-seq analysis was carried out on DMF-treated cells, and validation was done in two different HCC cell lines. Changes in subcellular structures were analyzed with electron microscopy. The expression of Bcl-xL, Nrf2, p-STAT3 and p-AKT were evaluated by immunoblotting.ResultsTumour growth of HCC was significantly lower in the DMF group compared to the control group (605.84 ± 65.06 mm3 and 232.1± 57.14 mm3, p < 0.01). Molecular and morphological studies revealed significant perturbation in the mitochondria accompanied by disruption of the NRF2/Bcl-xL axis. Enforced Bcl-xL expression in HCC cells markedly reversed DMF-induced apoptosis and induction of mesenchymal-to-epithelial transition. Notably, we also identified the activation of unfolded protein response as a potential marker for poor response. More strikingly, DMF synergistically enhanced sorafenib’s antitumor effect both in vitro, in zebrafish and mice xenograft models.ConclusionCollectively, our results demonstrate new mechanism insights into the antitumor effects of DMF and support the design of novel therapeutic options for HCC treatment.
ceRNA Network Analysis Shows That lncRNA LGALS8-AS1 Upregulates DCTPP1 To Promote Breast Cancer Progression Through Sponge miR-125b-5p
Authors: Zhang Jiayin , Qu Yuan , Hou Tingting , Ge Wanbao , Zhang Shanyong 
Published: November 2021

Abstract
Background: Breast cancer is the most common female cancer in the world. Many scholars have devoted themselves to elucidating the pathogenesis of Breast cancer. In the past, dCTP pyrophosphatase 1 (DCTPP1) was thought to be overexpressed in several cancers. However, The mechanism by which DCTPP1 is regulated by non-coding RNA in Breast cancer and its relationship with immune infiltration have not been elucidated.Results: In this study, reliable databases from the Cancer Genome Atlas (TCGA) and Gene Expression Integration (GEO) showed that the expression of DCTPP1 in Breast cancer tissues was higher than in normal tissues. Bioinformatics analysis showed that DCTPP1 was negatively correlated with the expression of hsa-miR-125b-5p in BRCA,The expression of LncRNA LGALS8-AS1 is positively correlated with the expression of DCTPP1, and negatively correlated with the expression of hsa-miR-125b-5p. Therefore, we speculate that lncRNA LGALS8-AS1 promotes tumor progression through sponge hsa-miR-125b-5p and maintains the overexpression of DCTPP1 in Breast cancer. The survival analysis of 3 genes showed that the overexpression of DCTPP1 and LGALS8-AS1 is related to the poor prognosis of patients. By analyzing the relationship between DCTPP1 and immune infiltration, we found that the high copy of DCTPP1 is related to the infiltration of CD8+ T cells, and the high expression of DCTPP1 is related to the infiltration of CD4+ T cells in basal-like Breast cancer. DCTPP1 is positively correlated with the expression of immune checkpoint B7-H3.Conclusion: LNC LGALS8-AS1 can upregulate DCTPP1 by sponging with hsa-miR-125b-5p. DCTPP1 can be used as a new prognostic marker for B7-H3 antibody treatment of breast cancer.
Lead Exposure Induces Structural Damage, Digestive Stress, Immune Response and Microbiota Dysbiosis in Intestine of Silver Carp (Hypophthalmichthys Molitrix)
Authors: Liu Haisu , Zhang Hang , Zhang Sanshan , Wang Anli , Fu Shengli 
Published: November 2021

Abstract
Lead (Pb) is one of the most common toxic heavy metals in water, and it can cause harm to aquatic animals and humans when released into the environment. In the present study, the effects of Pb exposure on the morphology, digestive enzyme activity, immune function and microbiota structure of silver carp (Hypophthalmichthys molitrix) intestines within 96 h were detected. Moreover, the correlation between them was analyzed. The results showed that Pb exposure could severely damage the intestinal morphology on the one hand, including significantly shortening the intestinal villi’s length, increasing the goblet cells’ number, causing the intestinal leukocyte infiltration, and thickening the intestinal wall abnormally, and on the other hand, increasing the activity of intestinal digestive enzyme (trypsin and lipase). In addition, the mRNA expressions of structure-related genes (Claudin-7 and villin-1) were down-regulated, and the immune factors (IL-8, IL-10 and TNF-α) were up-regulated after Pb exposure. Furthermore, data of the MiSeq sequencing showed that the abundance of membrane transport, immune system function and digestive system of silver carp intestinal microbiota was decreased, and the cellular antigens was increased. Finally, the canonical correlation analysis (CCA) found that there were correlations between silver carp’s intestinal microbiota and intestinal morphology and immune factors. In conclusion, it is speculated that Pb may damage the intestinal barrier of silver carp, leading the microbiota dysbiosis, which further affects the intestinal immune and digestive function.
Near Centimeter-scale FT-MIR Spectrometer based on NZIM Perfect Absorption using Inverted TanCirc Conformal Mapping Geometry
Authors: Swett Dwight W. 
Published: November 2021

Abstract
A long sought objective of MEMS research within the oil & gas industry has been the realization of “FT-IR on a chip,” which could hold the potential to migrate laboratory grade chemical spectroscopy into downhole sensor suites. A fundamental obstacle to this research has been the cooling demands of conventional technologies which conflict with the miniaturized sensor volumes required in downhole logging systems and environmental conditions that routinely exceed 125 • C. Near centimeter scale spectroscopic devices are required by a majority of downhole sensor suites, which stands in stark contrast to multiple-decimeter size conventional devices. Here we report a near-centimeter scale FT-MIR ATR spectrometer compatible with downhole volumetric and temperature constraints. The spectrometer is based upon a high-temperature broadband mid-infrared metasurface detector/source combination derived from a geometric inversion of a set of conformal mapping contours. The metasurface elemental structure is derived from a geometric inversion of the canonical TanCirc conformal mapping contours and was found to exhibit a near zero index metamaterial (NZIM) behavior over a spectral range of interest for downhole chemical spectroscopy. The NZIM properties of the metasurface lead to an absorption phenomenon characterized by surface plasmon resonances which confine the absorption mechanism within the ultrathin (λ /300) metasurface plane and make the absorption properties of the microbolometer design relatively insensitive to the material properties of the remaining laminae. This unusual feature allows the metasurface to be integrated on a single VO 2 material thermometric layer and operated at elevated downhole temperatures despite corresponding to the VO2 metal-insulator-transition (MIT) region. Within this transition region however the VO2 layer exhibits a substantially beneficial property in that the VO 2 layer exhibits more than an order of magnitude enhancement in its ambient thermometric properties, leading to an uncooled microbolometer design with predicted maximum detectivity D * = 1.5 × 10 10 cm √ Hz/W and noise equivalent difference temperature NEDT of 1 mK at a modulation frequency of 500 Hz. A sub-millimeter scale thermal infrared source with intrinsic mid-infrared band-limited emission is formed from the same cellular geometric building block, enabling the spectrometer miniaturization. These performance parameters compare well with lower tier laboratory grade FT-MIR spectroscopic instruments and could represent a significant step in the effort towards deploying miniaturized high-temperature mid-infrared spectroscopy into oilfield downhole logging applications.
Bone Mineral Density Attenuation in Obstructive Sleep Apnea by Derived Computed Tomography Screening
Authors: Daniel Sharon , Cohen-Freud Yafit , Shelef Ilan , Tarasiuk Ariel 
Published: November 2021

Abstract
The association between obstructive sleep apnea (OSA) and bone mineral density (BMD) is poorly elucidated with contradictory findings. We retrospectively explored the association between OSA and BMD by examining abdominal computed tomography (CT) vertebrae images using clinical information. We included 315 subjects (174 with OSA and 141 without OSA) who performed at least two CT scans (peak voltage of 120 kV). Bone mineral density was attenuated in those with OSA and increased age. BMD attenuation was not associated with the apnea–hypopnea score, nocturnal oxygen saturation, or arousal index. A multivariate linear regression indicated that OSA is associated with BMD attenuation after controlling for age, gender, and cardiovascular diseases. Here, we report that OSA is associated with BMD attenuation. Further studies are required to untangle the complex affect of OSA on BMD loss and possible clinical implication of vertebra depressed fracture or femoral neck fracture.