bluuebunny/crossref_metadata_2025_split huggingface dataset:
66 Strategies to Improve Phosphorus Utilization in Growing Pigs: Depletion-repletion Protocols
Authors: Lautrou Marion , Pomar Candido , Schmidely Philippe , Létourneau-Montminy Marie-Pierre 
Published: May 2021

Abstract
To optimize the use of dietary P by pigs, 5 feeding strategies were studied in a 3-phase feeding trial on 240 pigs (initial bodyweight (BW) of 31 kg): 1) C-C-C providing 100% of digestible phosphorus (Pdig, 4.3 g/kg STTD) and calcium (Ca, 9.7 g/kg) requirement to maximize bone mineralization, 2) L-L-L 60% of the Pdig and Ca requirements of C-C-C, 3) Phyt-Phyt-Phyt (phosphate-free, with phytase, 750, 686, 390 FTU/kg), providing 60% of Pdig and Ca requirements in phase 1, then 100%, 4) and 5) C in phases 1 and 3, and 60% of the need for Pdig in phase 2, associated with 65% of the requirements for Ca (N) or 80% (H), namely C-N-C and C-H-C. The BW and bone mineral content (BMC) were measured at the beginning and end of each phase. The BMC gain (gBMC), average daily gain (ADG) and average daily feed intake (ADFI) were calculated by phase. In phase 1, ADG was lower in the Phyt group than the C group (1.05 vs 1.10 kg/d, P &lt; 0.01) and the BMC of group C and gBMC were higher than those of the Phyt and B groups (P &lt; 0.05). In phase 2, C-C and Phyt-Phyt groups had similar BMC due to higher gBMC in the Phyt-Phyt (27.1 vs 18.4 g/d, P &lt; 0.01). At the end of phases 2 and 3, C-C-C, C-N-C and C-H-C groups had similar BMC. The Phyt and B groups showed an increased phosphorus-use efficiency during phases 1 and 2 (+20% vs C). Phosphorus retention was also higher in the C-N-C and C-H-C groups, during the depletion in phase 2 (+24% vs C, P&lt; 0.05). These results showed the potential of a depletion-repletion strategy including free phosphate diet to reduce phosphorus intake and excretion without affecting final growth performance and bone mineralization because of increased minerals utilization efficacies.
62 Growth Performance and Mineral Status of 6 Kg Piglets Fed Reduced Levels of Dietary Calcium
Authors: Merriman Laura A , Wyatt Craig , Létourneau-Montminy Marie-Pierre , Rousseau Xaviere , Bussières Dan 
Published: May 2021

Abstract
Imbalances between calcium (Ca) and phosphorus (P) impair growth performance and bone mineralization. However, reducing dietary limestone may change the buffering capacity of feed in early nursery piglets, which may help prevent post-weaning diarrhea. An experiment was conducted to evaluate the impact of reducing Ca post weaning compared to recommendations outlined by NRC (2012) or a low P diet. Dietary treatments consisted of 1) Low Ca (LCa; Phase 1, 0.51% Ca and 0.47% STTD P), 2) NRC recommendations (NRC; Phase 1, 0.85% Ca and 0.42% STTD P), and 3) a recommendation lower in phosphorus (LP; Phase 1, 0.65% Ca and 0.36% STTD P). Each diet was fed over 4 phases. Piglets (n = 953; 276/275 Fast X PIC 800 genetics) were blocked by room, sex, and initial BW (6 kg). Feed intake and pig weights were recorded weekly. At 12 d and 41 d, blood was collected and Dual-X ray (DXA) measurements were taken using 8 piglets per treatment. Fecal scores were evaluated during wk 4 and 5. Data were analyzed using MIXED procedure of SAS (SAS Inst. Inc., Cary, NC). There were no differences observed in mortality, overall growth performance, plasma Ca and P, and scour scores at either time point. At 12 d, the bone mineral content was reduced (P = 0.001) in LP pigs compared to LCa and NRC. At 41 d, bone mineral content was reduced in NRC in comparison to LCa while LP was intermediate (P = 0.001). Plasma parameters showed an increased magnesium (Mg) and Ca:Mg in LCa (P &lt; 0.01) that can be related to bone resorption to face Ca hypocalcemia. Pigs were healthy with no enteric challenges, limiting the ability to observe a benefit in fecal scores. In conclusion, piglets can maintain growth and bone mineralization through a short-term limestone removal program.
63 Effect of a Novel Consensus Bacterial 6-phytase Variant on Mineral Digestibility and Bone Ash in Young Growing Pigs Fed Diets with Low and High Soluble Limestone
Authors: Velayudhan Deepak E , Kumar Arun , Dersjant-Li Yueming 
Published: May 2021

Abstract
The effect of a novel consensus bacterial 6-phytase variant (PhyG) on total tract digestibility (ATTD) of minerals and bone ash was evaluated in piglets fed diets with low and high soluble limestone (LSLM and HSLM, 69 and 92% solubility at 5 min). For each limestone, 8 diets were formulated: an inorganic phosphate-free negative control (NC) diet based on wheat, corn, soybean-meal, canola-meal and rice-barn [0.18% standardized total tract digestible (STTD) P and 0.58% Ca], NC supplemented with 250, 500, 1,000 or 2,000 FTU/kg of PhyG, or with monocalcium phosphate (MCP) to formulate 3 positive controls with 0.21, 0.27, 0.33% STTD P, and 0.64, 0.70 and 0.75% Ca, respectively. In total 128 pigs (12.8 ± 1.33 kg, n=8) were adapted for 14 d followed by 4 d of fecal collection. Femurs were collected on the last day. Data were analyzed as one-way ANOVA and means separated by Tukey test. In addition, a 2 × 4 factorial analysis was performed to test the effect of limestone solubility at different MCP levels in control diets, a 2 × 5 factorial analysis was performed to determine the effect of limestone solubility at different phytase levels. Phytase dose-response was analyzed by orthogonal polynomial. Across phytase diets, HSLM reduced (P &lt; 0.05) ATTD Ca and P compared with LSLM. A consistent negative effect on ATTD P was also observed with HSLM in control diets. Across limestones, increasing phytase increased (P &lt; 0.05) ATTD Ca and P in a linear or exponential manner. Limestone solubility had no impact (P = 0.69) on bone ash. PhyG linearly increased (P &lt; 0.05) bone ash and 250 FTU/kg of PhyG maintained bone ash compared to PC. In conclusion, limestone solubility showed a negative impact on ATTD P and Ca, the novel consensus phytase improved ATTD P and Ca regardless of limestone.
64 Total Replacement of Inorganic Phosphorus by a Novel Consensus Bacterial 6-phytase Variant in Grower Pigs Fed Corn-soybean Meal-based Diets
Authors: Velayudhan Deepak E , Gracia Marta , Marchal Leon , Dersjant-Li Yueming 
Published: May 2021

Abstract
The efficacy of a novel consensus bacterial 6-phytase variant (PhyG) to totally replace dietary inorganic phosphorus (Pi) was evaluated in grower pigs fed diets with reduced net energy (NE) and digestible amino acids (AA), using growth performance and total tract digestibility (ATTD) of nutrients as outcome measures. A total of 352 growing pigs (23.4 ± 1.3 kg) were randomized to 4 dietary treatments with 8 pigs/pen and 11 pens/treatment. Diets were corn, soybean meal, distiller’s dried grains with solubles and wheat middlings based, formulated by phase (Grower 1, 25 to 50 kg and Grower 2, 50 to 75 kg). The positive control (PC) diet provided adequate energy and nutrients. A negative control diet was formulated without Pi (0.12% STTD P) and reduced Ca (-0.12 to -0.13 percentage points), NE (-32 kcal/kg) and essential AA (-0.15 to 0.3 percentage points) vs. PC. The NC was supplemented with 500 or 1,000 FTU/kg of PhyG. Data were analyzed by analysis of variance and orthogonal polynomial were used for linear response to increasing PhyG. Nutrient reductions in the NC reduced (P &lt; 0.05) average daily gain (ADG) during both phases and overall, increased (P &lt; 0.05) feed conversion ratio (FCR) and tended to reduce (P &lt; 0.1) feed intake during Grower 1 and overall, vs. PC. Supplementation of PhyG showed linear improvement (P &lt; 0.05) in FCR during Grower 1 and ADG during Grower 2 and PhyG at 1,000 FTU/kg maintained a similar growth performance compared to PC. Addition of PhyG improved (P &lt; 0.001) ATTD P and tended to improve ATTD of DE (P &lt; 0.1) in linear manner. In conclusion, the novel consensus phytase can replace Pi and compensate for the reduction of Ca, NE and digestible AA in grower pig diets with equal performance compared to the nutrients adequate positive control.
61 Effects of Reducing the Concentration of Ca and P and Increasing Microbial Phytase on Gastric Ph, Fecal Score, Growth Performance, and Bone Ash of Weanling Pigs
Authors: Lagos Vanessa , Bedford Mike R , Stein Hans H 
Published: May 2021

Abstract
An experiment was conducted to test the hypothesis that lowering dietary Ca and P reduces gastric pH and diarrhea of weanling pigs, but microbial phytase overcomes negative effects of low Ca and P on growth performance and bone ash. A total of 320 weanling pigs (6.35 ± 0.87 kg) were allotted to 8 corn-soybean meal-based diets in a randomized complete block design with 5 pigs/pen. Two phase 1 (d 1 to 14) control diets contained 100 or 50% of total Ca and digestible P relative to the requirement, and 6 diets in which 500, 2,000, or 16,000 units/kg of phytase was added to each control diet were formulated. Common diets were fed in phases 2 (d 15 to 27) and 3 (d 28 to 42). Fecal scores were recorded in phase 1 and growth performance data were recorded within each phase. Gastric pH was measured in 1 pig/pen on d 14; on d 14 and 42, the right femur of 1 pig/pen was collected. Data were analyzed using contrast statements in SAS. Results indicated that during phase 1, lowering Ca and P did not reduce gastric pH or fecal score, but the 50%-diets reduced (P &lt; 0.05) average daily gain and average daily feed intake of pigs compared with the 100%-diets (Table 1). Phytase above 500 units/kg increased (P &lt; 0.05) gain:feed ratio and tended (P &lt; 0.10) to decrease gastric pH. Pigs fed the 50%-diets had reduced (P &lt; 0.05) bone ash at d 14 and 42 compared with pigs fed the 100%-diets regardless of phytase inclusion level. In conclusion, reducing Ca and P in diets for weanling pigs does not decrease gastric pH or fecal score, but compromises growth performance and bone mineralization. However, super-dosing of phytase increases G:F of pigs regardless of dietary Ca and P concentration.
98 Buttiauxella Phytase Improves Growth Performance of Weanling Pigs Fed Corn, Soybean Meal, and Canola Meal Based Diets
Authors: Rundle Carly M , Dersjant-Li Yueming , Hillen Barthold , Oliveira Maryane S Sespere Faria , Stein Hans H 
Published: May 2021

Abstract
An experiment was conducted to test the hypothesis that a Buttiauxella phytase expressed in T. reesei can compensate the reduction in standardized total tract digestible (STTD) P, Ca, digestible energy (DE), and standardized ileal digestible (SID) AA in diets for pigs without impaired growth performance. A positive control diet (PC) was formulated to meet the requirement for STTD P and total Ca (0.33% STTD P and 0.60% Ca). A negative control diet (NC) was formulated with 0.18% STTD P and 0.45% Ca and with reduction of DE by 43 kcal/kg and SID AA by 0.01–0.02% vs. PC. Adding 250, 500, or 1,000 phytase units (FTU) per kg to the NC diet for a total of 5 experimental diets formulated three additional diets. A total of 155 pigs (BW: 8.77 ± 1.38 kg) were allotted to the 5 treatments, and there were 8 replicate pens per treatment. The experiment was conducted for 21 d. Pigs were weighed at the beginning and conclusion of the experiment, and daily feed allotments were recorded. Average daily gain (ADG), average daily feed intake (ADFI), and gain: feed ratio (G:F) were calculated for each treatment. Data were analyzed using contrast statements to compare PC and NC, PC and phytase, and to determine linear and quadratic effects of phytase. Results indicated that pigs fed the NC diet had reduced (P &lt; 0.05) ADG and G:F compared with pigs fed the PC diet (Table 1). Adding phytase to NC increased ADFI (linear, P &lt; 0.05) and ADG (quadratic, P &lt; 0.05) with the greatest values for the 1,000 FTU treatment. Including phytase in the diet also resulted in a quadratic increase (P &lt; 0.05) in G:F. In conclusion, Buttiauxella phytase may be included in diets with reduced nutrients and energy for weanling pigs without compromising pig growth performance.
96 Nutrient Digestibility of Toasted Soybean Meal and Untoasted Soybean Meal Extruded at Different Temperatures by Weanling Pigs
Authors: Milani Natália C , Paula Vinicius R C , Azevedo Cândida P F , Sedano Anderson A , Junior Hélio Moreira , Duarte Dave H A , Ruiz Urbano S Santos 
Published: May 2021

Abstract
The apparent and standardized ileal digestibility (AID and SID) of AA and CP and the apparent total tract digestibility (ATTD) of toasted soybean meal (SBM) and untoasted soybean meal (USM) extruded at 86°C (USM86), 120°C (USM120) and 149°C (USM149) were evaluated. USM was extruded mixed with corn starch (396.5g/kg USM and 603.5g/kg corn starch) in a single-screw extruder (MEX 250, Manzoni, Campinas, Brazil). Thirty-six barrows (7.69±0.89 kg BW) were fed a N-free diet, or four diets composed of 30% of each ingredient, as the only source of N, substituting for starch in N-free diet. Pigs were fed at 2.8 x maintenance (2.8 x 106 kcal digestible energy/kg BW0.75) for 10 days (5 d adaptation, 5 d feces collection), and on day 11 piglets were euthanized for ileal digesta collection. A randomized block design was used, with 8 replicates, using the pig as the experimental unit. ANOVA was performed and means were separated using Tukey test (5%). Increasing extrusion temperatures reduced trypsin inhibitors concentrations from 28.24 in USM to 3.51, 1.37, and 1.12 mg/g CP in USM86, USM122, and USM137, respectively, and 2.37 mg/g CP was found in SBM. USM86 showed lower (P &lt; 0.05) ATTD of DM (8–13%), GE (2–4%), and CP (12–16%) compared to SBM, USM120 and USM149, which did not differ (P &gt;0.05). The ATTD of EE of SBM was 17–19% higher (P &lt; 0.05) than of USM86 and USM120, which were similar (P &gt; 0.05) to each other and greater (P &lt; 0.05) than that of USM149. Extrusion at 86oC was insufficient to inactivate anti-nutritional factors and resulted in reductions (P &lt; 0.05) of 25–29% and 27–31% in CP and AA AID and SID compared to SBM, USM120, and USM149, which were, in general, similar (P &gt; 0.05). Soybean meal extruded at 120 and 139°C presented a similar nutritional value to toasted soybean meal.
99 Evaluation of IFTA NBS Alone and in Combination with Zinc Oxide on Antibiotic Free Programs During Nursery
Authors: Silva Gustavo , Knopf Brandon , Peterson Beau A , Pie Julia , Domenech Carlos , Díez David , Tesouro Anna 
Published: May 2021

Abstract
The objective was to evaluate the effect of IFTA NBS on productive performance in pigs from nursery until the end of 1st grow-finish phase. Total of 1,200 pigs (PIC 337 x 1050; PIC, Hendersonville, TN) were allotted in a randomized complete block design (RCBD) and assigned to blocks based on weight and sex, random allocated to pens with 25 pigs/pen. Diet changes occurred in 4 phases: N1 lasting 7 days, N2 14 days, N3 21 days and F1 21 days. Animals were random assigned to 4 treatments: T1) zinc oxide (ZnO) during N1 (3000 ppm) and N2 (2500 ppm) with no antibiotics; T2) carbadox at 50 g/ton in N1 and N2; T3) IFTA NBS at 500 g/ton during N1, N2 and N3; and T4) IFTA NBS at 500 g/ton during N1, N2 and N3 along with ZnO in N1 (3000 ppm) and N2 (2500 ppm). Treatments were fed a common diet during F1. Outcomes were analyzed as RCBD using SAS PROC GLIMMIX with pen as experimental unit, treatment as main effect and block as random effect. There were significant differences (P &lt; 0.05) in weights and average daily gain (ADG) between T2 and T3 during N2. Significant differences on average daily feed intake (ADFI) were observed between T1 and T2 vs. T3 during N2, and between T2 and T3 during N3. During nursery period (N1-N3), ADG and ADFI were different (P &lt; 0.05) between T2 and T3. By the end of the trial, a significant difference in gain:feed between T2 and T3 was detected. Under the conditions of this study, IFTA NBS in combination with ZnO could replace carbadox as it obtained similar weight and FCR during nursery. In addition, the response on gain:feed during the first grow-finish phase deserves further investigation since suggests a beneficial residual effect of IFTA NBS.
125 Effects of Dietary Fiber Supplementation in Late Gestation on Farrowing Characteristics and Sows and Litters Performance
Authors: Valadares Willian R , Leal Leonardo A , de Almeida Kevin , Perondi Dani , Kummer Rafael , Vier Carine M , Lu Ning , Orlando Uislei A , Ulguim Rafael R , Bortolozzo Fernando P , Mellagi Ana Paula G 
Published: May 2021

Abstract
Our objective was to evaluate the effects of dietary fiber supplementation in late gestation on farrowing characteristics and sow and piglet’s performance. On d 105 of gestation, a total of 420 sows were selected according to body condition score, caliper units and parity (0 to 6), and randomly assigned to one of two treatments in a completely randomized design. Treatments included 1) Low fiber diet (LF) with 2.02% crude fiber; and 2) High fiber diet (HF) with 5.12% crude fiber. The LF diet was based on corn and soybean meal with 3.26 Mcal of ME/kg and 0.60% SID Lys. The HF diet was corn-soybean meal-based with 10% soy hulls, containing 3.13 Mcal/kg and 0.60% SID Lys. Sows in both treatments were fed 1.8 kg/d. Fecal score was daily evaluated until farrowing. Farrowing duration and birth weight of born alive and stillborn piglets were recorded. The farrowing was manually assisted when the birth interval was longer than 30 min. Data were analyzed by the GLIMMIX procedure of SAS, and comparisons were performed with Student’s t-test at 5% of significance. Sows fed HF diet had fewer days with dry feces compared to those fed LF diet (P = 0.008). No evidence for treatment effects were observed on farrowing duration (P = 0.25) and farrowing assistance (P = 0.09). Sows fed HF diet had greater number of total piglets born (P = 0.01), but there was no evidence (P &gt; 0.05) for treatment differences on the percentage of piglets born alive, stillborn piglets, or mummified fetuses. Piglets from sows fed LF diet had a greater birth weight than those from sows fed HF diet (P = 0.04). No evidence for differences were observed between treatments for pre-weaning mortality, number and weight of weaned pigs (P &gt; 0.05). In conclusion, the HF diet did not show positive effects on farrowing duration, farrowing assistance, and sow and piglet performance.
122 A Mechanistic Model of Growth and Amino Acid Deposition in the Pregnant Sow: Model Development, Evaluation, and Application
Authors: Ramirez-Camba Christian D , Levesque Crystal L 
Published: May 2021

Abstract
A mechanistic model was developed with the objective to characterize weight gain and essential amino acid (EAA) deposition in the different tissue pools that make up the pregnant sow: placenta, allantoic fluid, amniotic fluid, fetus, uterus, mammary gland, and maternal body were considered. The data used in this modelling approach were obtained from published scientific articles reporting weights, crude protein (CP), and EAA composition in the previously mentioned tissues; studies reporting not less than 5 datapoints across gestation were considered. A total of 12 scientific articles published between 1977 and 2020 were selected for the development of the model and the model was validated using 11 separate scientific papers. The model consists of three connected sub-models: protein deposition (Pd) model, weight gain model, and EAA deposition model. Weight gain, Pd, and EAA deposition curves were developed with nonparametric statistics using splines regression. The validation of the model showed a strong agreement between observed and predicted growth (r2 = 0.92, root mean square error = 3%). The proposed model also offered descriptive insights into the weight gain and Pd during gestation. The model suggests that the definition of time-dependent Pd is more accurately described as an increase in fluid deposition during mid-gestation coinciding with a reduction in Pd. In addition, due to differences in CP composition between pregnancy-related tissues and maternal body, Pd by itself may not be the best measurement criteria for the estimation of EAA requirement in pregnant sows. The proposed model also captures the negative maternal Pd that occurs in late gestation and indicates that litter size influences maternal tissue mobilization more than parity. The model predicts that the EAA requirements in early and mid-gestation are 75, 55 and 50% lower for primiparous sows than parity 2, 3 and 4+ sows, respectively, which suggest the potential benefits of parity segregated feeding.
120 Impact of a Two-phase Lactation Feeding Program on Farrowing and Weaning Performance of Sows
Authors: Blomme Allison K , Weihs Nick , Jolliff James , Kellner Trey A 
Published: May 2021

Abstract
The U.S. Pork Industry uses a one-phase lactation feeding program based on logistical constraints and ease. The objective of this experiment was to quantify the sow performance differences between a one-phase and two-phase lactation feeding program to allow pork producers to calculate the economics of switching to a two-phase lactation feeding program. 257 gilts and sows (PIC 1050; Hendersonville, TN) were randomly assigned to a two-phase or one-phase lactation feeding program. Animals on the one-phase treatment were fed a typical lactation diet (2.55 Mcal/kg NE, 12.7% NDF, 0.99% SID Lys) for the duration of the experiment. Animals on the two-phase treatment were fed a high-fiber, lower-lysine transition diet (2.35 Mcal/kg NE, 18.5% NDF, 0.65% SID Lys) from the time they were loaded into the farrowing room until switching to the lactation diet on d 3 post-farrowing. From loading to farrowing, animals on both treatments were fed 1.82 kg/d, split between 2 meals at 630 and 1530 h. Daily feed amount was increased on the farrowing day and the 2 subsequent days (2.73, 4.09, 5.45 kg) until allowed ad libitum access to the lactation feed for both treatment groups on the third day after farrowing. Data were analyzed using Proc MIXED (SAS 9.4; Cary, NC) with treatment as the main effect and sow as the experimental unit. Comparing treatments, no significant differences on total born (one-phase = 16.2 vs. two-phase = 16.0, pigs/litter), live born (one-phase = 14.6 vs. two-phase = 14.4, pigs/litter), stillborn rate (one-phase = 8.1% vs. two-phase = 7.4%), number weaned (one-phase = 12.1 vs. two-phase = 11.9, pigs/litter), or weaning weight (one-phase = 5.67 vs. two-phase = 5.71, kg/pig; P ≥ 0.37) were detected. In conclusion, a two-phase lactation feeding program did not impair sow performance can be utilized to reduce lactation feed costs by $0.42/female/lactation cycle.