bluuebunny/crossref_metadata_2025_split huggingface dataset:
Recommendation on Advanced Molecular Testing in Hematolymphoid Malignancies in the Veterans Population
Authors: Neff Jadee L , Mosse Claudio A. , Wang-Rodriguez Jessica , Ahmed Sara , Kelley Michael 
Published: November 2021

Abstract
The Veterans Health Administration is the largest integrated provider of cancer services in the nation, with approximately 50,000 new cancer cases diagnosed each year. The goal of the VA's National Oncology Program is to establish a System of Excellence in cancer care with the help of tools, resources, programs, and best practices across the enterprise to ensure every veteran, regardless of location, receives the same high level of cancer care and access to precision diagnostics. This is mediated through a variety of programs, including the National TeleOncology Service and the National Precision Oncology Program which includes standardized clinical pathways for molecular testing, a second opinion service on diagnostic consultations, test result interpretation and treatment recommendations, as well as a molecular oncology tumor board.
The VA National Oncology Program and the Pathology and Laboratory Medicine Program offices have collaborated on a recommended guideline for advanced laboratory and molecular testing algorithm in hematologic diagnosis. We aim to offer best practice for all VA providers who must decide the type of ancillary tests at the time of initial clinical presentation in order to gain prognostic information and guide therapy. The recommendations include traditional laboratory tests, such as morphology, flow cytometry, and immunohistochemistry, as well as the use of fluorescent in situ hybridization (FISH), karyotype, polymerase chain reaction (PCR), and next generation sequencing (NGS). An overview of the draft algorithm for acute leukemias and chronic myeloid neoplasms is presented in Table 1; details will be discussed at the annual meeting.
Conclusion: The joint effort from VA Oncology and Pathology presents advanced testing algorithms in a variety of hematolymphoid malignancies as a systematic approach to hematopathology diagnosis, classification, as well as offering prognostic and therapeutic information. The recommendations across VA Healthcare would ensure standardized care for all VA patients with hematologic malignancy to benefit from the latest advances in precision medicine.
Figure 1 Figure 1.

Disclosures
Neff: Spring Discovery: Consultancy, Ended employment in the past 24 months; EUSA Pharma: Speakers Bureau; Enzyvant: Consultancy.
Epidemiology of Aggressive NK-Cell Leukemia in the United States: A SEER Population-Based Study
Authors: Amisha Fnu , Malik Paras , Konda Manojna , Kakadia Sunilkumar , Fugere Tyler , Mukherjee Akash 
Published: November 2021

Abstract
INTRODUCTION
Aggressive NK-cell leukemia ( ANKL) is a systemic lymphoproliferative disorder of natural killer (NK) cells frequently associated with Epstein-Barr virus (EBV) which has been grouped under histiocytic/dendritic neoplasms in the 2008 WHO classification of hematopoietic neoplasms. Due to rarity of diagnosis, the current available literature is limited to case reports and case series. This population-based study using Surveillance Epidemiology and End result program (SEER) is the largest to explore demographic characteristics, survival patterns and long-term outcomes in patients with ANKL in the United States.

MATERIALS AND METHODS
We utilized SEER 18 November 2020 submission database to select all patients (&gt; 1 years of age) diagnosed from 2000-2018 with ANKL using International Classification of Diseases for Oncology edition 3 (ICD-O-3) code of 9948/3. Patients were divided into various cohorts based on age (&lt;60 years, 60-79 years, &gt;80 years), sex, race (Caucasians, African American, Asian/Pacific islander and American Indians/Alaska natives) and median household income of county of residence (&lt; $ 50,000 vs &gt; $50,000). We used SEER*stat to calculate age adjusted incidence rate using 2000 US standard population. Kaplan Meier curve was utilized to calculate 5-year overall survival. Cox proportional hazard model was used for multivariate analysis of factors associated with survival and p&lt;0.05 was considered significant.
RESULTS
A total of 140 patients were identified with ANKL. The median age at diagnosis is 58 years. The crude, age-adjusted to 2000 US standard population and age-specific incidence rate of HCD in Unites states is &lt; 1/100, 000 respectively. The incidence in males is 1.9 times that of females- 92 males (65.7%) and 48 females (34.2%). Between 2001-2018, minimum number of cases were diagnosed in 2010 (n=2).Out of the cohort, 107 patients were White (76.4%), 21 patients were Asian or Pacific islander (15 %), 9 patients were African Americans (6.4%) and 3 patients were American Indian/Alaska native (2.1%). The median overall survival was 7 months (95% CI; 0-16). Survival rates at 1year, 2 years and 5 years were found to be 45.3% , 37.1 % and 31.1% respectively [Figure 1]. 5 year overall survival rates are as follows- Whites ( 69.7% ), Blacks ( 63.1%), American Indian (59.9%), Asian or Pacific Islander ( 66.8%). On multivariate analysis, black race was associated with poor outcomes (p 0.008), whereas sex, income and age had no significant effect on cancer outcomes.
CONCLUSIONS
Our study shows that ANKL is a rare hematological malignancy in general population with a poor median survival of less than one year. Males are twice more likely to be affected than females with poor outcomes in africo-americans.
Figure 1 Figure 1.

Disclosures
No relevant conflicts of interest to declare.
Ophthalmic Rosai–Dorfman disease: a multi-centre comprehensive study
Authors: Alzahem Tariq A. , Cruz Antonio Augusto , Maktabi Azza M. Y. , Chahud Fernando , Alkatan Hind 
Published: November 2021

Abstract
Background
To provide basic demographic information and clinicopathologic features of ophthalmic Rosai–Dorfman disease (RDD) with a literature review.

Methods
A multi-centre retrospective case series reviewing all patients with histopathologically confirmed ophthalmic RDD at three tertiary eye care centres between January 1993 and December 2018.

Results
Eleven eyes of eight patients with histopathologically confirmed ophthalmic RDD were included, with equal numbers of males and females. The median age was 40.25 years (range: 26.6–72.4). Two patients had familial RDD. The orbit was the most commonly involved site (90.9% eyes). One patient (one eye) presented with a scleral nodule, anterior uveitis and cystoid macular oedema. Visual acuity ranged from 20/25 to light perception. Six patients had an extra-nodal ophthalmic disease, and the remaining two had an associated submandibular lymphadenopathy (nodal RDD).

Conclusions
Ophthalmic RDD can be the only manifestation of this systemic disease, with the orbit being the most commonly involved site, exhibiting bone destruction, intracranial and/or sinus involvement and variable degree of visual loss. Ophthalmic familial RDD represent a severe form with a malignant course. Steroid monotherapy may be inadequate to control orbital RDD; thus, combined treatment is usually necessary. A comprehensive approach to assessment and management is recommended.
Metabolome and microbiome of chronic periapical periodontitis in permanent anterior teeth: a pilot study
Authors: Huang Yun , Zhou Peng , Liu Siqi , Duan Wei , Zhang Qinqin , Lu Ying , Wei Xin 
Published: November 2021

Abstract
Background
Periapical periodontitis is a common oral inflammatory disease that affects periapical tissues and is caused by bacteria in the root canal system. The relationship among the local metabolome, the inflammatory grade, and the type and abundance of microorganisms associated with periapical periodontitis is discussed in this study.

Methods
The inflammatory grades of periapical samples from 47 patients with chronic periapical periodontitis in permanent anterior teeth were determined based on the immune cell densities in tissues subjected to haematoxylin and eosin staining. The metabolome was evaluated using ultrahigh-performance liquid chromatography-quadrupole time-of-flight mass spectrometry, followed by principal component analysis and orthogonal partial least squares discriminant analysis. The microbiome was accessed using 16 S rRNA high-throughput sequencing. The differences in the metabolomes and microbiomes of the periapical periodontitis samples were assessed using Spearman’s correlation analysis.

Result
N-acetyl--glucosamine, -tryptophan, -phenylalanine, and 15 other metabolites were identified by the comparison between samples with severe inflammation and mild or moderate inflammation. Four amino acid metabolism pathways and one sugar metabolism pathway were associated with the inflammatory grade of periapical periodontitis. The abundance of Actinomycetes was negatively correlated with the abundance of glucosamine (GlcN), while the abundance of Tannerella was positively correlated with the abundance of -methionine.

Conclusions
The local metabolome of periapical periodontitis is correlated with the inflammatory grade. The abundance of the local metabolites GlcN and -methionine is correlated with the abundance of the major microorganisms Actinomycetes and Tannerella, respectively.
Factors related to turnover intention among staff of elderly caring social organizations in Anhui Province, China: a cross-sectional study
Authors: Chen Xuefei , Tang Ling , Liu Liu , Bai Zhongliang , Chen Ren 
Published: November 2021

AbstractObjectiveTurnover intention of employees in elderly caring social organizations has a significant impact on elderly care service delivery. This study investigated the associated factors of turnover intention among employees of elderly caring social organizations in Anhui Province, China.MethodsA total of 605 participants were selected using a multi-stage stratified random sampling method. A self-administered questionnaire was used to collect information on socio-demographic, social support, and turnover intention from the participants. The data was analyzed through descriptive statistical analysis, one-way variance analysis, Spearman correlation analysis, and multiple linear regression were used to analyze the factors related to turnover intention.ResultsResults of our study showed that the total score of turnover intention, turnover intention I (possibility of quitting a current job),turnover intention II (motivation to find other jobs) and turnover intention III (obtaining the external possibility of work) were 8.84, 2.32, 2.38, and 4.14, respectively. Social support negatively correlated with turnover intention I and turnover intention II. However, it showed positive correlation with turnover intention III and total turnover intention scores; turnover intentionI (coefficient: − 0.082), turnover intention II (coefficient: − 0.071), turnover intention III (coefficient: 0.19), Total score of turnover intention (coefficient: 0.093). Ethnic group, age, education level, and job satisfaction were associated with turnover intention.ConclusionImprovement of social support play an important role in reducing the turnover intention of employees in elderly caring social organizations. It is important to increase organizational commitment and strengthen psychological empowerment, combined with decreasing job burnout for stability.
Treatment effect of nafamostat mesylate in patients with COVID-19 pneumonia: study protocol for a randomized controlled trial
Authors: Moon Kyunglan , Hong Kyung-Wook , Bae In-Gyu 
Published: November 2021

Abstract
Background
This study is designed to evaluate the main hypothesis that nafamostat mesilate with standard therapy improves the severity and mortality rate in patients with COVID-19 pneumonia.

Methods
We conduct a randomized, open type, multi-institute/center, 2-group clinical trial with COVID-19 pneumonia patients in Korea. Eighty four patients with COVID-19 pneumonia are randomly assigned to intervention group or control group. Patients in intervention group receive the standard therapy with a dose of 0.1 to 0.2 mg/kg/h (2.4 to 4.8 mg/kg/day) of nafamostat mesilate. Patients in control group receive the standard therapy such as lopinavir/ritonavir, hydroxychloroquine, oxygen therapy, non-invasive and invasive ventilator, antibiotic therapy, renal-replacement therapy, and extracorporeal membrane oxygenation (ECMO). The primary outcome is proportion of patients with clinical improvement as defined by live discharge from hospital or a decline of 2 categories on the seven-category ordinal scale of clinical status, as well as secondary outcome comprised change in National Early Warning Score, duration of hospitalization, incidence of new-non-invasive ventilation or high flow oxygen use or ventilator, mortality at day 28, viral load change, and adverse events.

Discussion
Our study contributes to the establishment of therapeutic strategy in COVID-19 pneumonia by evaluating the therapeutic effect and safety of nafamostat mesilate.

Trial registration
ClinicalTrials.gov NCT04418128. Registered on 5 June 2020.
Unexpected similarity between HIV-1 reverse transcriptase and tumor necrosis factor binding sites revealed by computer vision
Authors: Eguida Merveille , Rognan Didier 
Published: November 2021

AbstractRationalizing the identification of hidden similarities across the repertoire of druggable protein cavities remains a major hurdle to a true proteome-wide structure-based discovery of novel drug candidates. We recently described a new computational approach (ProCare), inspired by numerical image processing, to identify local similarities in fragment-based subpockets. During the validation of the method, we unexpectedly identified a possible similarity in the binding pockets of two unrelated targets, human tumor necrosis factor alpha (TNF-α) and HIV-1 reverse transcriptase (HIV-1 RT). Microscale thermophoresis experiments confirmed the ProCare prediction as two of the three tested and FDA-approved HIV-1 RT inhibitors indeed bind to soluble human TNF-α trimer. Interestingly, the herein disclosed similarity could be revealed neither by state-of-the-art binding sites comparison methods nor by ligand-based pairwise similarity searches, suggesting that the point cloud registration approach implemented in ProCare, is uniquely suited to identify local and unobvious similarities among totally unrelated targets.
Seismic Retrofitting of RC Circular Columns Using Carbon Fiber, Glass Fiber, or Ductile PET Fiber
Authors: Choi Donguk , Hong Seongwon , Lim Myung-Kwan , Ha Sang-Su , Vachirapanyakun Sorrasak 
Published: November 2021

AbstractThe effectiveness of seismic retrofitting using three different fibers—carbon fiber (CF), glass fiber (GF), polyethylene terephthalate (PET) fiber—and a fiber combination of aramid fiber (AF) and PET fiber (called hybrid fiber reinforced polymer (HF)) wrapped on reinforced concrete (RC) circular columns was experimentally evaluated. A total of 11 RC circular columns were tested: three control columns and eight retrofitted columns in three different test groups. The purpose of fiber wrapping was flexural strength improvement as well as enhancement of rotational capacity in the plastic hinge region. Mechanical properties of CF, GF, AF, and PET were first defined; that is, CF, GF, and AF exhibited linear stress–strain behavior with limited ultimate strain capacity typically less than 3%, while ductile PET exhibited as much as 15% strain and non-linear stress–strain behavior with a very low elastic modulus. In the RC column tests, all three different fibers and the AF + PET fiber combination were effective in enhancing the strength and ductility but resulted in different structural behaviors and failure modes depending on the fiber type and the fiber amount used. The column sections were then analytically studied by section analysis using the behavior of confined concrete, the non-linear relationship of fiber-reinforced polymer (FRP), and the actual material properties of reinforcement. The analytical and experimental results revealed that ductile PET is beneficial, as it demonstrates more ductile behavior with a degree of strength enhancement similar to that of CF and GF.
Microbiota in mesenteric adipose tissue from Crohn’s disease promote colitis in mice
Authors: He Zhen , Wu Jinjie , Gong Junli , Ke Jia , Ding Tao , Zhao Wenjing , Cheng Wai Ming , Luo Zhanhao , He Qilang , Zeng Wanyi , Yu Jing , Jiao Na , Liu Yanmin , Zheng Bin , Dai Lei , Zhi Min , Wu Xiaojian , Jobin Christian , Lan Ping 
Published: November 2021

Abstract
Background
Mesenteric adipose tissue (mAT) hyperplasia, known as creeping fat is a pathologic characteristic of Crohn’s disease (CD). The reserve of creeping fat in surgery is associated with poor prognosis of CD patients, but the mechanism remains unknown.

Methods
Mesenteric microbiome, metabolome, and host transcriptome were characterized using a cohort of 48 patients with CD and 16 non-CD controls. Multidimensional data including 16S ribosomal RNA gene sequencing (16S rRNA), host RNA sequencing, and metabolome were integrated to reveal network interaction. Mesenteric resident bacteria were isolated from mAT and functionally investigated both in the dextran sulfate sodium (DSS) model and in the Il10 gene-deficient (Il10−/−) mouse colitis model to validate their pro-inflammatory roles.

Results
Mesenteric microbiota contributed to aberrant metabolites production and transcripts in mATs from patients with CD. The presence of mAT resident microbiota was associated with the development of CD. Achromobacter pulmonis (A. pulmonis) isolated from CD mAT could translocate to mAT and exacerbate both DSS-induced and Il10 gene-deficient (Il10−/−) spontaneous colitis in mice. The levels of A. pulmonis in both mAT and mucous layer from CD patients were higher compared to those from the non-CD group.

Conclusions
This study suggests that the mesenteric microbiota from patients with CD sculpt a detrimental microenvironment and promote intestinal inflammation.
Evaluating the effects of Bt protein Vip3Aa on immature Propylea japonica (Thunberg) (Coleoptera: Coccinellidae)
Authors: Ali Intazar , Zhang Shuai , Sajjad Asif , Khaliq Ghulam , Basit Muhammad , Bugti Ghulam Ali , Abbas Hafiz Tassawar , Abbas Naeem , Cui Jin-Jie 
Published: November 2021

Abstract
Background
Genetically modified (GM) crops have become a common tool for managing insect pests of economic importance. However, before their commercialization, bio-safety evaluation on non-target arthropods such as pollinators and biological control agents is a pre-requisite to save services rendered by them in agro-ecosystem.

Results
In the present experiment, the potential adverse effects of Bt protein (Vip3Aa) were studied on the life table parameters (pupation and emergence rates, 7-day larval weights, larval development time, and adult weight) of a predatory beetle, Propylea japonica (Thunberg) (Coleoptera: Coccinellidae) in the laboratory. P. japonica was exposed to Bt protein incorporated in synthetic diet at the rate of 200 µg/g of fresh weight of diet. Temporal stability, bioactivity and the uptake of insecticidal protein were confirmed by enzyme-linked immunosorbent assay and a sensitive insect bioassay. The results indicated that pupation and adult emergence rates did not differ significantly from that of negative control (pure synthetic diet). Similarly, the fresh weight of male and female adults emerged in Vip3Aa treatment (2.87 mg and 3.86 mg, respectively) did not vary significantly from that of the negative control treatment (2.66 mg, 3.75 mg). However, the time taken by the larvae to complete their development (12.70 days) was significantly extended than that of the negative control (10.21 days). In contrast, the tested life-table parameters of P. japonica were adversely affected when fed on synthetic diet containing boric acid (positive control) indicating experimental fitness to detect adverse effects.

Conclusions
On the basis of the results, it is suggested that GM crops expressing Bt protein (Vip3Aa) are safe for non-target insects such as P. japonica and are compatible with other biological programs for the integrated management of insect pests.
Framework for automated sorting of neural spikes from Neuralynx-acquired tetrode recordings in freely-moving mice
Authors: Strohl Joshua J. , Gallagher Joseph T. , Gómez Pedro N. , Glynn Joshua M. , Huerta Patricio T. 
Published: November 2021

Abstract
Background
Extracellular recording represents a crucial electrophysiological technique in neuroscience for studying the activity of single neurons and neuronal populations. The electrodes capture voltage traces that, with the help of analytical tools, reveal action potentials (‘spikes’) as well as local field potentials. The process of spike sorting is used for the extraction of action potentials generated by individual neurons. Until recently, spike sorting was performed with manual techniques, which are laborious and unreliable due to inherent operator bias. As neuroscientists add multiple electrodes to their probes, the high-density devices can record hundreds to thousands of neurons simultaneously, making the manual spike sorting process increasingly difficult. The advent of automated spike sorting software has offered a compelling solution to this issue and, in this study, we present a simple-to-execute framework for running an automated spike sorter.

Methods
Tetrode recordings of freely-moving mice are obtained from the CA1 region of the hippocampus as they navigate a linear track. Tetrode recordings are also acquired from the prelimbic cortex, a region of the medial prefrontal cortex, while the mice are tested in a T maze. All animals are implanted with custom-designed, 3D-printed microdrives that carry 16 electrodes, which are bundled in a 4-tetrode geometry.

Results
We provide an overview of a framework for analyzing single-unit data in which we have concatenated the acquisition system (Cheetah, Neuralynx) with analytical software (MATLAB) and an automated spike sorting pipeline (MountainSort). We give precise instructions on how to implement the different steps of the framework, as well as explanations of our design logic. We validate this framework by comparing manually-sorted spikes against automatically-sorted spikes, using neural recordings of the hippocampus and prelimbic cortex in freely-moving mice.

Conclusions
We have efficiently integrated the MountainSort spike sorter with Neuralynx-acquired neural recordings. Our framework is easy to implement and provides a high-throughput solution. We predict that within the broad field of bioelectronic medicine, those teams that incorporate high-density neural recording devices to their armamentarium might find our framework quite valuable as they expand their analytical footprint.
Hammertime!
Authors: Lange Jennifer 
Published: November 2021

Abstract
This memoir recounts Barbara Hammer's relationship to the Wexner Center for the Arts and its Film/Video Studio residency program, which supported a number of her films between 1994 and 2018. It offers some personal insight into the evolution of Hammer's final work, Evidentiary Bodies (2018), from single-channel video to multichannel video installation. The author describes working with Hammer during the last year of her life and explains the process of organizing the 2019 exhibition Barbara Hammer: In This Body at the Wexner Center for the Arts, which premiered the installation of Evidentiary Bodies and included additional works in other mediums and made throughout Hammer's career around themes of illness, aging, and death.