bluuebunny/crossref_metadata_2025_split huggingface dataset:
Extracellular Matrix-Liposomes: A Novel Vehicle for Tissue Regenerative Wound Healing
Authors: Lee Keel Yong , Nguyen Huong , Setiawati Agustina , Nam So-Jung , Kim Minyoung , Ko Il-Gyu , Jung Won Hee , Parker Kevin Kit , Kim Chang-Ju , Shin Kwanwoo 
Published: April 2021

Abstract
The unfolded states of fibronectin (FN) subsequently induce the formation of the extracellular matrix (ECM) fibrillar network, which is necessary to generate new substitutive tissues. Here, we demonstrate that negatively charged small unilamellar vesicles (SUVs) qualify as candidates for FN delivery due to their remarkable effects on the autonomous binding and unfolding of FN, which leads to increased tissue regeneration. In vitro experiments revealed that the FN-SUV complex remarkably increased the attachment, differentiation, and migration of fibroblasts. The potential utilization of this complex in vivo to treat inflammatory colon diseases is also described based on results obtained for ameliorated conditions in rats with ulcerative colitis (UC) that had been treated with the FN-SUV complex. Our findings provide a new ECM-delivery platform for ECM-based therapeutic applications and suggest that properly designed SUVs could be an unprecedented FN-delivery system that is highly effective in treating UC and other diseases.
Transmission characteristics of allexiviruses by the eriophyid mite, Aceria tulipae (Keifer) (Acari: Eriophyidae) from naturally mixed infected garlic (Allium sativum L.)
Authors: Mansouri Faten , Richert-Pöggeler Katja R. , Lewandowski Mariusz , Ryšánek Pavel 
Published: April 2021

Abstract
The transmission characteristics of members of the genus Allexivirus to leek (Allium porrum L.) by its eriophyid mite vector, Aceria tulipae (Keifer), were studied. Prior to conducting transmission tests, colonies of nonviruliferous A. tulipae were established on healthy leek seedlings. A single A. tulipae transmitted the viruses with up to 50 % efficiency but transmission efficiency increased when > 10 mites per plant were used. Allexiviruses were acquired by A. tulipae after a minimum acquisition access period (AAP) of 30 minutes, whereas transmission tests suggest that a one hour inoculation access period (IAP) was needed for successful transmission. Allexiviruses were transmitted from garlic to leek plants by A. tulipae and mixed infections by more than one virus were observed. ShVX, GarV-A, -C, -D, and -B were detected in most inoculated plants, whereas other members of the genus (GarV-E, -X, and GarMbFV) were found only occasionally. None of the mites that originated from eggs deposited on infected plants transmitted allexiviruses, indicating that the viruses are not transmitted transovarially. No latent period was demonstrated. Taken together, these data suggest a semipersistent mode of transmission of Allexivirus members by A. tulipae. The output of this study will assist in the better management of the vector and the associated diseases.
Integrated Dual-DFB PIC for High-Purity THz Carrier Generation Enabling Ultrafast THz Wireless Communications
Authors: Jia Shi , Lo Mu-Chieh , Zhang Lu , Ozolins Oskars , Udalcovs Aleksejs , Kong Deming , Pang Xiaodan , Guzman Robinson , Yu Xianbin , Xiao Shilin , Popov Sergei , Chen Jiajia , Carpintero Guillermo , Morioka Toshio , Hu Hao , Oxenløwe Leif 
Published: April 2021

Abstract
With the explosive growth of global wireless data traffic, the Terahertz band (0.3–10 THz) is promising for ultrafast wireless communications, due to the enormous available bandwidth [1]. Photonic generation of THz carriers displays extremely large tunable range and modulation bandwidth, making it nearly ideal for THz communications. However, the current photonics-based THz carrier generators are based on discrete bulky components [2] with high cost and energy consumption, which hinder them from practical applications. Here, we present an injection-locked heterodyne source based on generic foundry-fabricated photonic integrated circuits (PIC) attached to a photo-mixing uni-travelling carrier photodiode (UTC-PD), generating high-purity THz carriers for high-speed and long-distance wireless communication. The generated THz carrier can span from 0 to 1.4 THz, determined by the tunable wavelength spacing between the two distributed feedback (DFB) modes within the range 0-10.7 nm. We show that a generated 0.4 THz carrier transmits a record-high single-channel net rate of 131 Gbit/s over 10.7 m of wireless distance with only − 24 dBm emitted THz power, by employing 16-QAM-OFDM modulation and a nonlinear equalization technique. To the best of our knowledge, this is the highest data rate for a single-channel THz wireless transmission and requires the lowest THz power/bitrate/distance. The scheme of the monolithic dual-DFB PIC based THz generation shows a great potential for fully integrated, cost-effective and energy-efficient THz transmitters.
Mettl3 promotes oxLDL-mediated inflammation through activating STAT1 signaling
Authors: Li Zhenwei , Xu Qingqing , Huangfu Ning , Chen Xiaomin , Zhu Jianhua 
Published: April 2021

Abstract
Atherosclerosis (AS) is the main cause of cerebrovascular diseases, and macrophages act important roles during the AS pathological process through regulating inflammation. Modification of the novel N(6)-methyladenine (m6A) RNA is reported to be associated with atherosclerosis, but its role in AS is largely unknown. The aim of this study was to investigate the role and mechanism of m6A modification in oxidised low-density lipoprotein (oxLDL) triggered inflammation in macrophages during AS. Dot blot, Immunoprecipitation, western blot, Rip and Chip experiments were used in our study. We find oxLDL stimulation significantly promotes m6A modification level of mRNA in macrophages and knockdown of Methyltransferase-Like Protein 3 (Mettl3) inhibits oxLDL-induced m6A modification and inflammatory response. Mettl3 promotes oxLDL-induced inflammatory response in macrophages through regulating m6A modification of Signal transducer and activator of transcription 1 (STAT1) mRNA, thereby affecting STAT1 expression and activation. Moreover, oxLDL stimulation obviously enhances Mettl3 interacting with STAT1 protein to promote STAT1 transcriptional regulation of inflammatory factor expression in macrophages, which was also demonstrated in the monocytes from patients with angiographically proven coronary artery disease. These results indicate that Mettl3 promotes inflammatory responses through interacting with STAT1 protein and mRNA in macrophages, suggesting that Mettl3 may be as a potential target for the clinical treatment of atherosclerosis.
Overconstrained Cable-Driven Parallel Manipulators Statics Analysis based on Simplified Static Cable Model
Authors: Luan Phan Gia , Thinh Nguyen Truong 
Published: April 2021

Abstract
In recent years, cable-driven parallel manipulators (CDPM) become more and more interesting topics of robot researchers due to its outstanding advantages. Unlike traditional parallel robots, CDPMs use many flexible cables in order to connect the robot fixed frame and the moving platform instead of using conventional rigid links. Since cables used in CDPM is very light compared to rigid links, its workspace can be very large. Besides, CDPMs are often enhanced load capacity by adding redundant actuators. They also help to widen the singularity-free workspace of CDPM. On the other hand, the redundant actuators produce the underdetermined system i.e. the system has non-unique solutions. Moreover, the elasticity and bendability of flexible cable caused by self-weight and external forces act on it, resulting in the kinematic problem of CDPMs are no longer related to the geometric problem. Therefore, the system of CDPM become non-linear when the deformation of cable is considered. In this study, we introduce the simplified static cable model and use it to linearize the static model of redundantly actuated CDPM. The algorithm to solve the force distribution problem is proposed in Sect. 4. The static-workspace and the performance of those are analyzed in a numerical test.
Identification of the transcription factor, AFF4, as a new target of miR-203 in CNS
Authors: Li Shufang , Liang Xiaosheng , Zheng Yangwu , Li Linpeng , Cao Lin , Zou Yi 
Published: April 2021

Abstract
The expression of miR-203 was found elevated by preconditional treatment of isoflurane in cultured B35 cells as well as in the cerebral cortex of rats in our previous study. Recently, miR-203 was identified as a hub of a potential regulatory miRNA network in central nervous system. Overexpressing of miR-203 in the frontal cortex of C57BL/6J wild type mouse induced neurodegeneration by increasing the apoptotic pathway and neuron death. Here in this research, we reported that AFF4, a transcription factor, was identified as a new bona fida protein target of miR-203 in CNS. The miRNA:mRNA interaction of miR-203 and AFF4 was verified using Dural-luciferase assay. Down-regulated expression of AFF4 was induced by overexpressing miR-203 both in vitro and in vivo. Open field test, Y maze and Morris water maze test were conducted for the behavioral assessment of the mice with stereotactic injection of lentiviral vector overexpressing miR-203 in the hippocampus. No anxiety-like behavior or impaired cognition was noticed in these mice. Consistent with the results of the behavioral assessment, the electron micrograph and Nissl staining revealed no significant change in the synaptic density and no neuron injuries in the hippocampus of mice overexpressing miR-203, respectively. Given that miR-203 was found to target the proteins with opposite regulatory functions, our results indicated that instead of promoting neurodegenerative phenotype, a more profound function should be ascribed to miR-203 in regulating neuron behavioral activities and cognition. Neuron-type specific functions of miR-203 is likely to be executed via its various downstream protein interactors.
Analysis On The Association of Intraoperative Fluid Balance and Short-Term Outcomes After Radical Gastrectomy in Aged Patients
Authors: Cui Yin , Wu Yuhui , Han Xue , Zhu Beibei , Ma Zhengliang , Gu Xiaoping 
Published: April 2021

Abstract
Background: To observe the relationship between fluid balance and the short-term outcomes of aged patients after gastrectomy for gastric cancer in Nanjing Drum Tower Hospital.Methods: The clinical data of patients with gastrectomy for gastric cancer from January 2016 to December 2018 in Nanjing Drum Tower Hospital were retrospectively analyzed. According to the criteria of inclusion and exclusion, 691 patients who met the study conditions were analyzed according to intraoperative fluid balance recorded on patients who has undergone radical gastrectomy. Patients were classified into three fluid administration groups representing incremental quartiles of the primary exposure variable. Preoperative characteristics used for statistical adjustment included gender, age, weight, admission type, ASA degree. Operative factors included procedure duration, estimated blood loss, urine output, and so on. The primary outcomes included acute kidney injury (AKI).and postoperative respiratory complications (PRCs) Exploratory outcomes included length of stay, postoperative length of stay and total cost of hospitalization. The association between perioperative factors and AKI/PRCs in hospital was tested with multivariable logistic regression analyses.Results: 16 cases were diagnosed as AKI and 23 cases were diagnosed as PRCs. The association between intraoperative fluid balance and the incidence of acute kidney injury (AKI)/postoperative respiratory complications (PRCs) remained U-shaped but the difference was not statistically significant (P>0.05). After adjustment for potential confounders, lower urine output (P=0.017, OR=0.997,95%CI=0.994-0.999) and coronary heart disease (P=0.032, OR=4.867,95%CI=1.142-20.75) were independent predictor of AKI in aged patients after radical gastrectomy. Besides, coronary heart disease(OR=3.371,95%CI=1.021-11.129,P=0.049) and intestinal obstruction (OR=12.501,95%CI=3.058-51.107,P ＜0.0005) were independent predictor of PRCs in aged patients after radical gastrectomy.Conclusion: There were no significant association between the incidence of AKI or any other complications and intraoperative fluid balance during radical gastrectomy in aged patients. Lower urine output and coronary heart disease were independent predictors of AKI in aged patients after radical gastrectomy. Coronary heart disease and intestinal obstruction were independent predictors of PRCs in aged patients after radical gastrectomy.Trial registration: This study was approved by the Affiliated Drum Tower Hospital of Nanjing University (Registration number: 2018-162-01).
MiR-486-5p represses chondrocyte multiplication but facilitates their deaths in congenital microtia through aiming at H3F3B
Authors: Liu Ying , Zhang Ling , Dong Weiwei , Yang Qinghua 
Published: April 2021

Abstract
Background

MicroRNAs have been reported to play important roles in the development of external ear. The present study was designed to explore the expression patterns and biological function of miR-486-5p in congenital microtia.
Methods

Cartilage tissue specimens were collected from congenital microtia cases who received reconstructive ear surgeries, while the normal cartilage tissues from other otology surgeries were adopted as normal controls. The relative expression of miR-486-5p was investigated by quantitative-real time polymerase chain reaction. The potential target of miR-486-5p was explored by TargetScanHuman 7.2 and dual-Luciferase reporter assay. MTT and flow cytometry assays were adopted to investigate cell proliferation and apoptosis, respectively.
Results

The expression of miR-486-5p was significantly up-regulated in microtia cartilage tissues. Knockdown of miR-486-5p in microtia chondrocytes enhanced proliferation and inhibited apoptosis. H3F3B might be a target of miR-486-5p, and miR-486-5p could regulate proliferation and apoptosis of microtia chondrocytes by targeting H3F3B. Moreover, the enforced expression of H3F3B could re-turned the function of miR-486-5p in microtia chondrocytes.
Conclusion

Up-regulation of miR-486-5p may inhibit microtia chondrocytes proliferation and promote apoptosis by targeting H3F3B.
Heterosis for Biomass-Related Traits in Interspecific Triploid Hybrids of Willow (Salix Spp.)
Authors: Carlson Craig H. , Smart Lawrence B. 
Published: April 2021

Abstract
Species hybridization is key for the improvement of shrub willow (Salix spp.) bioenergy crops, because hybrids often display heterosis for yield. Development of high-yielding genotypes requires numerous broad attempts at hybridization followed by field evaluation and selection for stable performance. Selection of improved shrub willow varieties for use as a bioenergy crop involves evaluation of full-sib progeny in family-based selection trials. Improving the accuracy of evaluation would greatly improve the efficiency of selection. Heterosis for biomass yield in intra- and interspecific F1 and F2 shrub willow (Salix spp.) was examined utilizing a suite of biomass, foliar, and physiological traits collected over the course of 12 weeks in the greenhouse and over two years in the field. Triploid families generated from diploid S. viminalis and tetraploid S. miyabeana displayed the greatest levels of heterosis for harvestable biomass and biomass-related growth traits in the greenhouse and in the field. While intraspecific S. purpurea diploids exhibited low levels of heterosis for these traits, interspecific diploids produced moderate levels of heterosis in greenhouse experiments. Differences between greenhouse and field trial results can largely be explained by pest damage, which negatively impacted interspecific diploids. Heterosis for the traits that form the basis for biomass yield, including stem growth, foliar, and physiological traits, was quantified and family-level differences are discussed.
Molecular detection of Anaplasma phagocytophilum DNA in Olive Baboons and Vervet monkeys in Kenya
Authors: Masika Sophie Jerusa , Muchemi Gerald Mwangi , Kamau Joseph Muiruri , Abuom Tequiero , Mutura Samson Kamawe 
Published: April 2021

Abstract
BackgroundNonhuman primates (NHPs) occupy an important place in zoonotic spill-overs, serving as either reservoirs or amplifiers of multiple neglected tropical diseases including tick-borne infections. Anaplasmosis is caused by obligate intracellular bacteria of the family Anaplasmatacae. They are transmitted by Ixodid tick species and have a wide host range including wild animals, domestic animals and humans. The aim of this study was to establish the presence of Anaplasma phagocytophilum in Olive baboons and Vervet monkeys in Laikipia County, Kenya.ResultsA total of 164 whole blood samples, collected by USAID Predict II project from Laikipia County, 17 Kenya, were included in the study. These comprised of 146 samples from Olive baboons (Papio anubis) and 18 from Vervet monkeys (Chlorocebus pygerythrus) respectively from Mpala Research Center and Ol jogi Conservancy in Laikipia County. Using conventional Polymerase Chain Reaction (PCR), positive results for A. phagocytophilum in 26 Olive baboons and 4 Vervet monkeys were detected with primer sets EHR16SD/R. However, low sensitivity was observed with the p44 gene. The amplification of DNA template with the primer set p44 (p3709 5/ p4257 23 5) using nested PCR could not be obtained. Our results revealed the presence of A. phagocytophilum in Olive baboons and Vervet monkeys. This study found an overall prevalence of 18.3% for Anaplasma phagocytophilum. A distinct genotype of A. phagocytophilum was detected that was different from the others in the gene bank database.ConclusionThis study provides valuable information on the presence of A. phagocytophilum bacteria in Olive baboons and Vervet monkeys in Kenya. It indicates a need for future research to establish the public health implications of zoonotic A. phagocytophilum isolates and the role of nonhuman primates as reservoirs.
Augmented Reality Application for Laptop Assembly with Assembly Complexity Study
Authors: Hong Chiew Jin , Sung Aun Naa Aun 
Published: April 2021

Abstract
Augmented Reality (AR) in the assembly process will improve the user's experience by providing interactive instructions in real time. However, no previous application of AR guided assembly for laptops with a high level of assembly complexity has been developed. The research aims to develop an AR guided assembly application to provide instruction on the assembly of a laptop. The assembly complexity of the laptop was also investigated. The development of the AR application involves the creation of model target, 3D models and animations, and the development of user interface. The laptop assembly consists of ten steps. Each step comprises animated 3D models and text detailing the assembly instructions. Speech recognition has been used to navigate the assembly sequence. The AR application has successfully been developed for laptop assembly with an assembly complexity of 6.63. With the developed application, the performance of the laptop assembly can be accelerated.
Free-Space Optical Link Optimization in Visible Light Communication System
Authors: Emmanuel Ngene Chidi , Thakur Prabhat , Singh Ghanshyam 
Published: April 2021

Abstract
In this paper, a free-space optical link (FSOL) optimization in the visible light communication (VLC) is performed to improve the channel performance for signal strength and analysze the effect of signal failures in a communication system. The problem of uncertainty in the FSOL components for a better signal has prompted this study to check for an improved and enhance signal using bit error rate (BER) analyzer. The light emitting diode (LED) is used as a carrier for transmission of data as the optimization of system properties have concluded for limitations and working conditions. The noise has been added and removed to ascertain the channel behavior which in turn display an acceptable eye diagram for an improved signal. The acceptable eye diagram shows a better signal in the system. The optimization of FSOL will guarantee multi-mobile users of shared signal which will improve the signal errors, loss of power, weak signal, signal failure, loss of data etc. To fully characterize the FSOL, OptiSystem software tool has been used.
Elevated expression of IL-10RB acts as an independent unfavorable prognostic marker and predicts the polarization of tumor-associated macrophages in glioma
Authors: Fan Yaqiong , Peng Yichen , Chen Feng , Li Wenbin 
Published: April 2021

Abstract
Background: Interleukin-10 receptor B (IL10RB) is a subunit of the interleukin-10 receptor. As an important part of the interleukin-10 signaling pathway, IL10RB plays an essential role in the regulation of the immune response. However, studies of IL10RB in glioma are rare. The present study aimed to investigate the potential role of IL10RB in glioma.Methods: 611 glioma samples in TCGA dataset and 310 glioma samples in CGGA dataset were download and organized, then analyzed the clinical expression characteristics and prognostic value of IL10RB as well as its correlation with immune cell infiltration in these samples using R. Results: IL10RB expression was significantly higher in the glioma tissues than in the normal tissues and increased in gliomas, especially with malignant phenotype. The Kaplan-Meier survival analysis and Cox regression analysis indicated that IL10RB was significantly associated with poor prognosis in patients with glioma and could be used as an independent prognostic marker. Further functional analysis suggested that IL10RB was involved in immune responses, and high expression of IL10RB in the glioma tissue resulted in more infiltrated immune cells, especially macrophages. Moreover, IL10RB was found to be strongly correlated with marker genes of tumor-associated macrophages (TAMs) and M2 macrophages, but low or even no correlation with marker genes of M1 macrophages. In addition, there was also a significant association between IL10RB and immune checkpoints that suppress effective antitumor immune responses.Conclusions: Our results demonstrated that high expressed IL10RB was associated with the malignant progression of gliomas and poor prognosis, and it might be involved in the polarization of macrophages which play a crucial role in the immunosuppressive response of gliomas.
Post-Traumatic Stress Disorder and the Risk of Erectile Dysfunction: A Nationwide Cohort Study in Taiwan
Authors: Wang Sheng-Chiang , Chien Wu-Chien , Chung Chi-Hsiang , Tzeng Nian-Sheng , Liu Yia-Ping 
Published: April 2021

Abstract
BACKGROUND: This study aimed to investigate the association between posttraumatic stress disorder and the risk of developing erectile dysfunction.METHODS: In this population-based, retrospective cohort study, we used Taiwan’s National Health Insurance Research Database to analyze the patients who were newly diagnosed with posttraumatic stress disorder (PTSD) between 2000 and 2013, with a 1:3 ratio by age, and index year matched in the non-PTSD comparison group, for the risk of erectile dysfunction. RESULTS: In total, five out of 1,079 patients with PTSD developed erectile dysfunction, and three out of 3,237 patients in the non-PTSD group (47.58 vs 9.03 per 100,000 per person-year) developed erectile dysfunction. The Kaplan-Meier analysis showed that the PTSD cohort had a significantly higher risk of erectile dysfunction (log-rank, p<0.001). The Cox regression analysis revealed that the study subjects were more likely to develop an injury (HR: 12.898, 95% CI=2.453- 67.811, p=0.003) after adjusting for age, monthly income, urbanization level, geographic region, and comorbidities. Psychotropic medications in the patients with PTSD were not associated with the risk of erectile dysfunction.CONCLUSIONS: Patients who suffered PTSD had a higher risk of developing erectile dysfunction.