bluuebunny/crossref_metadata_2025_split huggingface dataset:
Puzzling and powering in the city: two case studies on collaborative research
Authors: Duiveman Robert 
Published: July 2021

Abstract
Cities are turning to urban living labs and research consortia to co-create knowledge that can better enable them to address pervasive policy problems. Collaborations within such practices help researchers, officials and local stakeholders find new ways of dealing with urban issues and developing new relations with one another. Interestingly, success in the latter is often closely related to accomplishing the former. Besides of analysing this phenomenon in terms of learning—as is common—this paper also delves into the power dynamics involved in collaborative knowledge development. This perspective contributes to a better understanding of how puzzling and powering are simultaneously involved in making research relevant to policy-making. By presenting two collaborative research consortia in the Netherlands, we demonstrate how developing knowledge involves both re-structuring problems and the urban practices involved in governing such problems. Collaborative research practices are predominantly concerned with learning as long as restructuring the problem leads to research findings that are meaningful to all actors. Power becomes manifest when one actor insists on restructuring (often reproducing) problems in a manner judged unacceptable by others. Analysis of two case studies will show how the familiar three faces of power express themselves in collaborative knowledge development. It is recommended that these new practices also require methods for better orchestrating power besides a methodology for successful structuring learning through collaborative research practices.
Behavior of the Central Intensity of Generalized Humbert-Gaussian Beams Against the Atmospheric Turbulence
Authors: Nossir Naima , Dalil-Essakali Latifa , Belafhal Abdelmajid 
Published: July 2021

Abstract
Based on the generalized Huygens-Fresnel integral in the paraxial approximation and on the Rytov theory, the analytical expression of the axial intensity distribution for the Generalized Humbert-Gaussian beams (GHGBs) propagating through a turbulent atmosphere is derived in this work. The results for the special cases of GHGBs are deduced from our study and illustrated numerically. The influence of the turbulent strength and the incident beam parameters on the propagation of these beams in the atmospheric turbulence is investigated and discussed in detail.
Evaluation of Antioxidant Potential and Efficacy of Terpenoid Rich Fraction of Phyllanthus Amarus (Schum and Thonn) Whole Plant in the Amelioration of High Salt Diet Induced Obesity.
Authors: Fasan Tope Israel , Olorunnisola Olubukola Sinbad , Adetutu Adewale , Ajilore Bamidele Stephen 
Published: July 2021

Abstract
Background

It’s overwhelmingly accounted that there is high prevalence of high salt diet related adiposity and metabolic antioxidant infringement worldwide. The present study thus aimed to establish the folkloric use of Phyllantus amarus by traditional practitioners and compliment orthodox medicine in the management of high salt diet driven adiposity and vehemently associated with pro-oxidant cascades. The principal focus was to fractionate, evaluate the lethal dose of terpenoid rich concentrate of the Phyllanthus amarus (Schum and Thonn) whole plant using up and down method and investigate the in vivo antioxidant potential and antiobesity of the rich concentrate in healthy adult male Sprague-Dawley rats fed with 8% high salt diet for 8weeks.
Results

The results of the study revealed that there was a significant (p < 0.05) weight gained (54.50%) in the experimental group fed with a high salt diet when compared with rats fed with normal chow (51.19%). However, rats co-administered with High salt diet (8%) and terpenoid rich fraction (TRF) of the whole plant (75, 100, 150mg/kg/body weight), had a significant weight recuperation in dose-dependent manner (50.86%, 48.13%, 43.25%) in comparism to groups fed with normal rat chow (51.19%), with corresponding significant (p < 0.05) and dose-dependent decrease in the concentration of oxidative marker product malondialdehyde (MDA) (9.38 ± 0.22, 7.92 ± 0.11, 6.03 ± 0.18) and dose-dependent metabolic improvement of both the non-enzymatic reduced Glutathione (GSH) (40.15 ± 0.04, 46.21 ± 0.01, 51.22 ± 0.03) and enzymatic catalase (25.22 ± 0.01, 30.31 ± 0.05, 38.52 ± 0.03) activities.
Conclusion

Therefore, the terpenoid rich fraction of Phyllanthus amarus (Schum and Thonn) whole plant could therefore be applied as a recuperative agent against obesity and prooxidant proliferation in high salt diet related pathologies.
Oceaniglobus Trochenteri sp. nov., Isolated from the Gut Microflora of top Shell (Trochus Maculatus Linnaeus).
Authors: Chen Zhuo , Yun Shuai-ting , Zhang Ming-jing , Yin Rui , Zhou Yan-Xia 
Published: July 2021

Abstract
A Gram-stain negative, non-flagellated, beige-pigmented, circular, catalase-positive, oxidase-positive bacterium, designated G4T, was isolated from gut microflora of top shell (Trochus maculatus Linnaeus) collected from Diwanggong market, Weihai, PR China. The novel isolate was able to grow at 4–42°C (optimum 25–33°C), pH 7.0–9.0 (optimum 6.5–7.0) and with 0.0–11.0% NaCl (optimum 2.0–3.0%, w/v). Analysis of 16S rRNA gene sequence revealed that strain G4T shared the highest 16S rRNA gene sequence similarities with Oceaniglobus YLY08T (96.6%), followed by Oceaniglobus indicus 1-19bT (95.3%). The genome of strain G4T, with 32 assembled contigs, was 4.5 Mb long with a G + C content of 65.3 mol%. DNA–DNA hybridization values of the isolate against the closely related type strains were far below the 70% limit for species delineation. The average amino acid identity, average nucleotide identity and digital DNA-DNA genome hybridization relatedness between strain G4T and the closely related members of the genus Oceaniglobus, Oceaniglobus indicus1-19bT and Oceaniglobus ichthyenteri YLY08T were 71.3, 76.4 and 20.0%, and 75.0, 76.3 and 19.4%. The major cellular fatty acid was summed feature 8 (C18:1ω7c and/or C18:1ω6c). The sole respiratory quinone detected was Q-10. The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol and phosphatidyldimethylethanolamine. The results of phenotypical, phylogenetic and biochemical analyses indicated that strain G4T represents a novel species in genus Oceaniglobus within the family Rhodobacteraceae, for which the name Oceaniglobus trochenteri sp. nov. is proposed. The type strain is G4T (= MCCC 1K04356T = KCTC 82506T).
miR-133a-3p Functions as a Tumor Suppressor in Colorectal Cancer by Targeting PFKFB3
Authors: Zhou Yanjie , Jiang Lu , Cai Yuanjun , Tang Wendong , Wang Ke 
Published: July 2021

Abstract
Background

Recent studies reveal that PFKFB3 plays an important role in tumorigenesis and tumor progression. Our study aims to identify an novel microRNA which can suppress the expression of PFKFB3 and to provide a potential target for tumor therapy.
Methods

Bioinformatics methods were implemented to explore the expression and clinical significance of PFKFB3 and miR-133a-3p in colorectal cancer (CRC). qRT-PCR was performed to detect PFKFB3, miR-133a-3p, KI67 and MMP9 mRNA expression, while western bot was carried out for the detection of protein expression of PFKFB3, miR-133a-3p, KI67 and MMP9. Bioinformatics analysis was used to predict the binding sites of miR-133a-3p on PFKFB3 3’UTR, while dual-luciferase assay was conducted to validate their binding relationship. CCK-8 assay, KI67 detection, Transwell assay and MMP9 detection were employed to measure CRC cell proliferative and invasive abilities.
Results

PFKFB3 expression is up-regulated in colorectal cancer, and is significantly associated with poor prognosis. Silencing PFKFB3 could inhibit the proliferation and invasion of colorectal cancer cells. miR-133a-3p is down regulated in colorectal cancer, which has diagnostic value for colorectal cancer. Dual luciferase assay confirmed that PFKFB3 was the direct acting site of miR-133a-3p. Overexpression of miR-133a-3p could significantly reduce the expression of PFKFB3 and inhibit the effect of PFKFB3 on the proliferation and invasion of colorectal cancer cells.
Conclusions

Our study suggested that miR-133a-3p functions as a novel tumor suppressor in colorectal cancer by targeting PFKFB3.
A global bibliometric and visualized analysis in the state of Pseudomonas aeruginosa efflux pump research
Authors: Zhang Ruiqin , Wang Siyang , Ren Jian , Li Zhen , Wang Hongxia , Zhi Tingting , Zhu Yuanyuan , Wang Yanhui , yao zhiqing , Zhang Huizi , DAUN jinju 
Published: July 2021

Abstract
OBJECTIVE. This study was designed with the goal of surveying the current status of the Pseudomonas aeruginosa efflux pump research field in an effort to explore current and future trends in this clinically relevant research area. METHODS. Bibliometric and visual analyses were used to conduct a global survey of the current status of Pseudomonas aeruginosa efflux pump research. All studies regarding Pseudomonas aeruginosa efflux that were published from 1994 to 2019 were retrieved from the Web of Science database, after which we conducted bibliographic coupling, co-authorship, co-citation, and co-occurrence analyses, and analyzed trends in Pseudomonas aeruginosa efflux pump research publications using the VOS Viewer software. RESULTS. We identified 2583 total publications that were included in this study. The number of relevant studies from around the world was found to be rising over time. The USA was identified as a central player in this research field at a global level, as studies from the USA had the most citations, the highest H-index values, and the greatest total link strength. Three primary research directions were identified through this analysis, including studies of efflux pump resistance mechanisms, transfer mechanisms, and inhibitors. CONCLUSIONS. Our visual analysis provides a quantitative overview for researchers who wish to quickly understand the past and current dynamics of the field of Pseudomonas aeruginosa efflux pump research. Overall, these findings suggest that future studies of efflux pump resistance mechanisms and efflux pump inhibitors may aid in overcoming clinical Pseudomonas aeruginosa resistance.
The Influence of Posterior Tibial Slope on the Mid-term Clinical Effect of Medial-pivot Knee Prosthesis
Authors: Shi Weipeng , Jiang Yaping , Zhao Xuan , Zhang Haining , Wang Yingzhen , Li Tao 
Published: July 2021

Abstract
Objective: To evaluate the effect of posterior tibial slope (PTS) on the mid-term clinical outcome following a medial-pivot (MP) prosthesis. Method: 233 patients from this hospital, who had undergone a total knee arthroplasty (TKA) with MP prosthesis between January 2015 and December 2015, were retrospectively included in this study. They were divided 3 groups according to postoperative PTS: A ≤ 5°; B 5-7° and C ≥ 7°. Multiple assessments were made on the patient postoperatively and recorded in the three groups, the measurements of this study included: The range of motion (ROM), knee scoring system (KSS), Western Ontario and McMaster universities osteoarthritis index (WOMAC), posterior condylar offset (PCO), joint line height and postoperative complications. Results: The average post-operative ROM for groups B and C were 108°and 110°respectively, this was significantly higher than that of group A (98°, P < 0.001). The WOMAC scores of patients in group C were significantly lower than those in groups A and B (p < 0.05). However, there were no significant differences in KSS, PCO, joint line height among the 3 groups (P>0.05). Only 2 cases of postoperative complications occurred in group C, these were recovered after operation. Conclusion: With an increase to PTS, the postoperative ROM can be significantly increased for the patient, however, the knee joint function will not be significantly improved, and the stability of knee joint will not be affected within the appropriate PTS.
Machine learning algorithms for the identification of potential non-syndromic intellectual disability associated miRNAs
Authors: Betancur Julián González , Guevara-Coto José , Romero Adarli 
Published: July 2021

Abstract
Background: Intellectual disabilities (IDs) are a group of developmental disorders with high phenotypic and genotypic heterogeneity. Association of genetic elements to IDs has typically been empirically accomplished, however recently, machine learning (ML) has proved to be an excellent instrument to elucidate these associations. miRNAs are short non-coding molecules that participate in spatiotemporal gene regulation, making them relevant for the understanding ID causality. Methods: In this study we used the BrainSpan spatio-temporal expression database to develop a series of machine learning predictors: SVM, RF, FF-ANN, and Stochastic Gradient Descent Classifier. These models were capable of recognizing gene expression profiles. The best classifier was used to label miRNAs associated with NS-IDs using the BrainSpan expression profiles. Results: The model with the best performance was a FF-ANN with 0.78 of F1-score, 0.78 of weighted recall and 0.78 of weighted precision. We used this model to identify miRNAs with high probability to be associated with NS-IDs using the spatio-temporal gene expression profile in the human brain. Labeled miRNAs that were annotated were associated with processes related to either IDs and-or neurodevelopmental processes. Conclusions: The development of a machine learning framework that identified potential NS-ID miRNAs represents an interesting approach for the identification of a potential list of on genes that could be subject for further experimental validation. This study also reinforces the potential of machine learning frameworks in their discovery of potential biomarkers that could improve disease detection and management.
Machine Learning-Guided Noninvasive Embryo Selection for Clinical in Vitro Fertilization Treatment to Avoid Wasting Potentially Qualified Embryos
Authors: Chen Li , Li Wen , Liu Yuxiu , Peng Zhihang , Cai Liyi , Zhang Ningyuan , Xu Juanjuan , Wang Liang , Teng Xiaoming , Yao Yaxin , Zou Yangyun , Ma Menglin , Liu Jianqiao , Lu Sijia , Sun Haixiang , Yao Bing 
Published: July 2021

Abstract
BackgroundThe success rates of in vitro fertilization (IVF) treatment are limited by the aneuploidy of human embryos. Pre-implantation genetic testing for aneuploidy(PGT-A) is often used to select embryos with normal ploidy but requires invasive embryo biopsy. MethodsWe performed chromosome sequencing of 345 paired blastocyst culture medium and whole blastocyst samples and developed a noninvasive embryo grading system based on the random forest machine-learning algorithm to predict blastocyst ploidy. The system was validated in 266 patients, and a blinded prospective observational study was performed to investigate clinical outcomes between machine learning-guided and traditional niPGT-A analyses. We graded embryos as A, B, or C using machine learning-guided niPGT-A analysis according to their euploidy probability levels predicted by noninvasive chromosomal screening. ResultsWe observed higher live birth rate in A- versus C-grade embryos (50.4% versus 27.1%, p=0.006) and B- versus C-grade embryos (45.3% versus 27.1%, p=0.022) and lower miscarriage rate in A- versus C-grade embryos (15.9% versus 33.3%, p=0.026) and B- versus C-grade embryos (14.3% versus 33.3%, p=0.021). The embryo utilization rate was significantly higher through machine learning strategy compared to the conventional dichotomic judgment of euploidy or aneuploidy in the niPGT-A analysis (78.8% versus 57.9%, p<0.001). We observed better outcomes in A- and B-grade embryos versus C-grade embryos and higher embryo utilization rates through machine learning strategies than traditional niPGT-A analysis. ConclusionThese results demonstrate that the machine learning-guided embryo grading system can optimize embryo selection and avoid wasting potential embryos.Trial registration﻿Chinese Clinical Trial Registry,ChiCTR-RRC-17010396.Registered 11 January 2017, http://www.chictr.org.cn/ChiCTR-RRC-17010396
Seasonal Dynamic of Cholecalciferol (D3) And Anti-Muellerian Hormone (AMH) With Impact On Ovarian Response And IVF/ICSI
Authors: Rogenhofer Nina , Jeschke Udo , von Schönfeldt Viktoria , Mahner Sven , Thaler Christian J. 
Published: July 2021

Abstract
Objective:Recent studies revealed intriguing associations between cholecalciferol (D3) and reproductive functions. Seasonal changes of D3 concentrations are well known, however they are not always considered in the context of reproductive functions. In this study, we analysed D3 serum concentration in IVF/ICSI patients with respect to seasonal 3-month quartiles and anti-Muellerian hormone (AMH) referring to the impact on Assisted Reproductive Technologies (ART) outcome. Materials and Research Methods:We studied 497 female patients, presenting between 2012 and 2018 for ART treatment in our fertility center. D3 as well as the AMH serum concentrations were measured at the beginning of the follicle stimulation (d 3-5 of menstrual cycles). Results were evaluated with respect to seasonal quartiles and outcome of the ART cycles. Results:D3 concentrations showed significant fluctuations within annual quartiles with a pronounced peak in August-October and a minimum in February-April (27.7mg/dl vs. 20.9mg/dl; p<0.0001). Similar seasonal dynamics were found for AMH (3.19ng/ml vs. 2.24ng/ml; p=0.013) and these were associated with significantly shorter stimulation periods during August-October (11.36d vs. 12.01d; p=0.044), higher number of fertilized oocytes between August-October (6.21 vs. 5.21; p=0.05) along with a trend towards higher numbers of Cumulus-Oocyte-complexes. However, no such differences were found for the numbers of MII-oocytes or pregnancy rates.Conclusion: Our data indicate, seasonal 3-month quartile variations of AMH concentrations and characteristics of ART, such as days of ovarian stimulation and number of fertilised oocytes. Highest AMH concentrations were found between August and October and this quartile was associated with highest D3 concentrations.
The Function Role of Ubiquitin Proteasome Pathway in the ER Stress-induced AECII Apoptosis during Hyperoxia Exposure
Authors: Zhu Yue , Ju Huimin , Lu Hongyan , Tang Wei , Lu Junying , Wang Qiuxia 
Published: July 2021

Abstract
Backgroud: Bronchopulmonary dysplasia (BPD) is a major cause of mortality and morbidity in premature infants, characterized by alveolar dysplasia and pulmonary microvascular remodeling. In the present study, we have investigated the functional roles of ubiquitin proteasome pathway (UPP) in BPD, and its relationship with endoplasmic reticulum stress (ER stress, ERS) mediated AECII apoptosis. Methods: A hyperoxia-induced BPD rat model was constructed and the pathologic changes of lung tissues were evaluated by Hematoxylin-Eosin staining. Cell apoptosis and protein expression were determined by TUNEL assay and Western blotting, respectively. Further reagent kit with specific fluorescent substrate was utilized to measure the activity of 20s proteasome. Meanwhile, AECII were cultured in vitro and exposed to hyperoxia. AECII apoptosis were measured by flow cytometry. In contrast, MG132 treatment was induced to explore ubiquitin proteasome pathway during hyperoxia exposure on AECII apoptosis and ERS sensors expression.Results: A significant increase in apoptosis and total ubiquitinated proteins expression were observed in BPD rats and AECII culture, and the change of UPP was associated with ERS. In order to confirm the role of UPP in AECII apoptosis of BPD, AECII cells were treated by MG132 with the concentration of 10 μmol/L under hyperoxia exposure. We found that the proteins expression of GRP-78, PERK, ATF4, ATF6 and CHOP, as well as AECII apoptosis were increased following MG132 treatment. Furthermore, the relatively up-regulated in the levels of total ubiquitinated proteins expression and 20S proteasome activity were correlated with increased ERS sensors expression. Conclusions: Our findings indicate that UPP may participate in the ERS-induced AECII apoptosis under hyperoxia condition.
Surgically Managed Symptomatic Intraspinal Lumbar Facet Synovial Cyst: Outcome of surgical treatment with resection and instrumented posterolateral fusion, a case series.
Authors: Lalanne Lyonel Beaulieu , Lemos Facundo Alvarez , Carmona Roberto Larrondo , Totera Juan Ignacio Cirillo , Montoya Andre Beaulieu , Wilson Jose Muñoz 
Published: July 2021

Abstract
PURPOSE: There is controversy regarding the treatment of symptomatic synovial cysts (SSC), specifically, the need for a concomitant fusion when surgical resection of the CS is required. We present a retrospective review of a series of patients treated for SSC of the lumbar region during the last 20 years by a single surgeon, analyzing the current available literature. METHODS: Retrospective review. The same surgical technique was applied to all patients. Demographic, clinical, surgical data and synovial cyst recurrence rate were recorded. Postoperative results reported by patients were documented according to the McNab score. RESULTS: 69 subjects, with mean follow-up of 7.4 years. 62% (43) were female, with a mean 57.8 years at the time of surgery. In 91.3% (63), the primary management was conservative for a minimum period of 3 months. All subjects underwent surgery due to the failure of conservative treatment. The segment most operated on was L4-L5 (63.77%). 91.3% (63) of the sample reported excellent and good and 6 subjects (8.6%) fair or poor results. There was no evidence of CS recurrence at the operated level. CONCLUSION: In SSC, it seems that conservative treatment is only effective in a limited number of patients and in the short term. Thus, the recommendation of a surgical indication should proceed as soon as the conservative management fails to result in significant symptom relief. Based on our results, we recommend, together with the resection of the cyst, the instrumentation of the segment to avoid its recurrence and the management of axial pain.