bluuebunny/crossref_metadata_2025_split huggingface dataset:
Rotational rice transplanting schedule to prevent water scarcity in West Sumatra
Authors: Wardiman D , Oue H , Irsyad F 
Published: December 2024

Abstract
Rice production in West Sumatra decreased due to the impact of climate change. One of the efforts to prevent water scarcity in the rice field is improving water management on the community scale. This study aimed to observe rice water management to prevent water scarcity. The research was conducted in the Tilatang Kamang subdistrict, Agam Regency, through field observation and interviews from November 2023 to April 2024. P3A Namuang Saiyo manages the water management for rice fields. Farmers used a rotational transplanting schedule to prevent water scarcity by cultivating rice 2-month intervals between the upstream, midstream, and downstream areas. The rice cultivated in 2023 shows yields in the areas of 3.48 t ha−1, 3.88 t ha−1, and 3.74 t ha−1, respectively, using a conventional transplanting system (CU) of 37 x 37 cm. However, other conventional transplanting systems help farmers manage fertilizer with sizes 70 x 18 x 53 cm (M1) and 12 x 37 x 37 cm (M2). The results show that CU obtained a higher yield by 3.88 t ha−1, followed by M2 (2.87 t ha−1) and M1 (2.23 t ha−1).
Local Taste versus Global Flavors: A Qualitative Study of Consumer Perceptions on Domestic and Imported Fruit Products
Authors: Ridel Saviola Timban Aimar , Rahman Saili Abdul , Gladys Jocom Sherly , Ruth Mandei Juliana , Mahirah Merali Mashitah 
Published: December 2024

Abstract
The global marketplace has become increasingly saturated with a diverse range of fruit products, posing significant challenges for marketers and businesses in understanding consumer behavior towards these products. This study aims to investigate consumers’ perception of domestic and imported fruits, employing a qualitative approach to explore the complexities of consumer preferences and decision-making processes in the fruit market. This research was conducted from July to September 2023 in Manado City, North Sulawesi Province, Indonesia. Sixty interviews were conducted, with participants selected through convenience sampling and the data was analyzed using qualitative analysis. The results reveal that consumer perceptions of domestic and imported fruit products are multifaceted, with safety concerns, flavor preferences, affordability, accessibility, and cultural influences playing significant roles. The findings of this study offer several practical implications for industry stakeholders and provide strategy to strengthen and enhance of both marketing of domestic and imported fruits.
The Influence of Nitrogen and Potassium in Boesenbergia rotunda Growth Dynamics
Authors: Wahid S , Samah S N A A , Yusuf N A , Rahayu M S , Hishamuddin F , Yazik Z H AB , Othman N M I , Abdullah F A 
Published: December 2024

Abstract

Boesenbergia rotunda, also referred as ‘Temu Kunci’ is a Zingiberaceae herb widely grown in Malaysia by vegetative techniques. The rhizome of ‘Temu kunci’ is extensively used in conventional medicine to treat gout, gastrointestinal issues, and other conditions. The objective of this study was to determine the growth dynamics of B. rotunda by establishing its phenological growth stages using the extended BBCH scale and assessing how nitrogen (N) and potassium (K) fertilization affected plant’s growth development and yield production. Rhizomes were planted in a 3 : 2 : 1 ratio into a mixed planting medium (topsoil, sand, and chicken dung). Eight combination treatments with four replications were used in this experiement, which was carried out using a Randomized Complete Block Design (RCBD) with two N levels and four K levels. The N levels were 60 and 120 kg N ha−1, and the K levels were 0, 60, 120, and 180 kg ha−1 for each N level, while the P level was kept constant at 70 kg ha−1. Fertilizers such as Urea, CIRP, and MOP were supplied four times during the planting period (32, 64, 95, and 125 DAS). Phenological, growth development and yield potential data were collected and analyzed using one-way and two-way analysis of variance (ANOVA). The result indicated that T2 at 60 N kg/ha and 60 K kg/ha was the optimal quantity of N and fertilization to increase growth and yield production of B.rotunda . Principal Growth Stage 0: Bud development, Principal Growth Stage 1: Leaf development, Principal Growth Stage 2: Formation of stems/tillering and Principal Growth Stage 6: Flowering were the four growth phases of B. rotunda that were also identified using the extended BBCH scale.
Insights into the molecular underpinning of type 2 diabetes complications
Authors: Singh Archit , Bocher Ozvan , Zeggini Eleftheria 
Published: December 2024

Abstract
Type 2 diabetes (T2D) complications pose a significant global health challenge. Omics technologies have been employed to investigate these complications and identify the biological pathways involved. In this review, we focus on four major T2D complications: diabetic kidney disease, diabetic retinopathy, diabetic neuropathy, and cardiovascular complications. We discuss advancements in omics research, summarizing findings from genetic, epigenomic, transcriptomic, proteomic, and metabolomic studies across different ancestries and disease-relevant tissues. We stress the importance of integrating multi-omics techniques to elucidate the biological mechanisms underlying T2D complications and advocate for ancestrally diverse studies. Ultimately, these insights will improve risk prediction for T2D complications and inform translation strategies.
Private Law, Statutory Powers and Environmental Disputes: Manchester Ship Canal Company v United Utilities (No 2)
Authors: Hariharan Jeevan 
Published: December 2024

Abstract
In Manchester Ship Canal Company Ltd v United Utilities Water Ltd (No 2) the UK Supreme Court unanimously held that the Water Industry Act 1991 did not prevent claims being brought in trespass or nuisance against United Utilities for foul water discharges into the Manchester Ship Canal. The judgment is significant because it opens the door to similar claims being brought against sewerage undertakers, which many had thought were statutorily blocked off following the House of Lords’ decision in Marcic v Thames Water Utilities Ltd. While the result of the decision has understandably been welcomed by environmental groups, this analysis argues that the Supreme Court’s reasoning reveals a more complex picture. In particular, the court’s refusal to overrule Marcic and the expansive way the court conceives of the private rights of property holders are matters for concern for anyone looking to private law as a way of remedying environmental harms.
The Long-Run Labour Market Effects of the Canada-U.S. Free Trade Agreement
Authors: Kovak Brian K , Morrow Peter M 
Published: December 2024

Abstract
This article assesses the long-run effects of the 1989 Canada-U.S. Free Trade Agreement on the Canadian labour market using matched longitudinal administrative data for the years 1984–2004. We simultaneously examine the labour market effects of increased export expansion and import competition, generally finding adverse effects of Canadian tariff cuts and favourable effects of U.S. cuts, though both effects are small. Workers initially employed in industries that experienced larger Canadian tariff concessions exhibit a heightened probability of layoffs at large firms, but little impact on long-run cumulative earnings. Lower earnings and years worked at the initial employer are offset by gains in other manufacturing industries, construction, and services. Canadian workers quickly transitioned out of industries facing import competition, with the bilateral nature of the FTA providing import-competing workers employment options in alternative manufacturing industries benefiting from larger U.S. tariff cuts.
Surgical Reconstruction of Unstable, Hypoplastic Fingertips With Allograft and BMP-2 Augmentation: Surgical Technique and Case Series
Authors: Hanson Zachary , Casey Peter , Lourie Gary M. 
Published: December 2024

Objective:
Unstable hypoplastic digits can lead to poor function due to instability with pinch and manipulation of objects. The primary goals of surgical reconstruction in these patients are to create 2 stable digits for pinch and improve finger opposition, pinch strength, and digit mobility. In this paper, we present a surgical technique and case series of pediatric patients who underwent surgical reconstruction of unstable/telescoping hypoplastic fingertips using bony allograft augmented with BMP-2.

Materials and Methods:
A surgical technique for reconstruction of unstable, hypoplastic fingertips was performed using a bony allograft augmented with BMP-2. The series included pediatric patients with hypoplastic digits with instability affecting grip-pinch strength and hand prehension.

Results:
Ten patients between the ages of 2 and 4 years were included, with 8 thumbs and 2 index fingers being reconstructed. All 10 patients developed clinically stable digits with parent-reported improvements in pinch-grip function including using a switch, opening a bag, and putting on clothes. Postoperative radiographic outcomes did not show any cases of bony overgrowth; 2 patients had evidence of bony resorption though this did not correspond to a significant change in finger length postoperatively.

Conclusion:
Hypoplastic fingertip reconstruction using bony allograft augmented with BMP-2 is a surgical option for the stabilization of unstable hypoplastic fingertips in congenital hand anomalies. The use of allograft augmented with BMP-2 eliminates donor site morbidity seen with autograft reconstructive options and may limit the concerns with healing/incorporation traditionally reported with allograft use in the literature.
A Physiotherapy Framework to Managing Long COVID: A Clinical Approach
Authors: Peirce Scott , Taylor Mitchell , Pollok Talia , Holtzhausen Samantha , Peirce Brooke , DeMars Jessica 
Published: December 2024

Purpose:
Individuals infected with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the virus that causes coronavirus disease 2019 (COVID-19), can experience ongoing, often debilitating symptoms after the acute infection known as Long COVID (LC). LC has profound medical, social, and economic consequences worldwide. Prevalence estimates vary, but it is estimated that 10% to 35% of people infected with SARS-CoV-2 develop LC. The World Health Organization endorses physiotherapy as a vital component in LC symptom management and stabilization. Cardiorespiratory physiotherapists are often involved in the management of patients with LC phenotypes such as post-exertional malaise/post-exertional symptom exacerbation, post-COVID interstitial lung disease, dysautonomia, breathing pattern disorders, and chronic cough. However, specific guidance is lacking regarding physiotherapy assessment and safe intervention strategies. In this review, we describe the relevant pathophysiology of the condition, report common clinical phenotypes, and propose a clinical framework for physiotherapy assessment and safe intervention strategies.
Interobserver Reliability of the 5-Point Deauville Score and SUV-Based Quantification of FDG PET/CT Scans in High-Risk Pediatric Hodgkin Lymphoma
Authors: Lee Inki , Milgrom Sarah A. , Im Hyung-Jun , Pei Qinglin , Renfro Lindsay , Hoppe Bradford S. , McCarten Kathleen M. , Schwartz Cindy L. , Cole Peter D. , Kelly Kara M. , Cho Steve Y. 
Published: December 2024

Purpose
In lymphomas, the Deauville score (DS) standardizes 18F-FDG PET treatment response assessments. We assessed interobserver agreement of the DS and developed a quantitative PET-based parameter to minimize subjectivity of response assessments.

Methods
PET scans performed within the Children’s Oncology Group AHOD0831 study were analyzed. One hundred one scans obtained after the first cycle of chemotherapy (PET1) and 83 obtained after the second cycle (PET2) were eligible for inclusion. Each scan was assigned a DS by 2 nuclear medicine radiologists and a Hodgkin lymphoma index (HLI), defined as the ratio of the tumor’s SUVmax or SUVpeak to the liver’s SUVmean. Cohen κ coefficient measured the rate of agreement of the DS assigned by the 2 radiologists. Receiver operating characteristic curves and the Youden index were used with the consensus DS to identify an optimal HLI cutoff value to distinguish PET-negative from PET-positive images. For each HLI cutoff value, sensitivity, specificity, and accuracy were calculated.

Results
Interobserver agreement in DS was moderate (PET1: κ = 0.480, P < 0.001; PET2: κ = 0.428, P < 0.001). On PET1, the optimal cutoff values for HLImax were 1.19 for DS2/DS3 and 1.78 for DS3/DS4, and for HLIpeak were 1.19 for DS2/DS3 and 1.44 for DS3/DS4. On PET2 images, the corresponding values were 1.18, 1.58, 0.94, and 1.26. The most accurate predictor was HLImax on PET2 when scans were dichotomized as DS1–DS3 versus DS4–DS5 (accuracy, 92.8%; sensitivity, 100%; specificity, 91.8%).

Conclusion
The HLI provides an objective, quantitative measure of disease response and thus may improve interreader agreement for lymphoma response assessments.
Reducing Intubations and Related Risks in Neonates with Retinopathy of Prematurity Undergoing Laser Photocoagulation
Authors: Quinones Cardona Vilmaris , Byrne Emma McNell , Arthur Novisi , Young Megan , Lavery Diane , Carroll Amanda , Joshi Swosti , Kehinde Folasade , Menkiti Ogechukwu 
Published: December 2024

Introduction:
Although associated with respiratory morbidity, elective endotracheal intubation (ETI) for laser photocoagulation for retinopathy of prematurity (ROP) is the standard practice at our institution, with 100% of patients undergoing preoperation ETI. To mitigate this risk, we strove to reduce the percentage of infants intubated for laser photocoagulation by 30% by June 2022.

Methods:
We assembled a multidisciplinary team and implemented a deep sedation guideline utilizing dexmedetomidine, fentanyl, and midazolam with noninvasive ventilation support for laser photocoagulation in January 2020. Outcome, process, and balancing measures tracked the efficacy and safety of the quality improvement project.

Results:
We reduced the percentage of infants requiring intubation for laser photocoagulation from 100% (8/8) to 10% (1/10). We reduced the average time to return to baseline respiratory status from 224.1 to 33.8 hours (9.3d to1.4 d). Cardiorespiratory index scores slightly increased (1 to 1.2), and pain scores remained unchanged after interventions.

Conclusions:
A multidisciplinary team approach using a deep sedation guideline and noninvasive ventilation can safely reduce the requirement for intubation during laser photocoagulation with a faster return to baseline respiratory status.
Pain in idiopathic scoliosis not associated with known genetic variants for pain
Authors: Cheng Tian , Diarbakerli Elias , Simony Ane , Østerheden Andersen Mikkel , Danielsson Aina , Kere Juha , Einarsdottir Elisabet , Gerdhem Paul 
Published: December 2024

Abstract

Introduction:
Back pain is common in idiopathic scoliosis. The aim of this study was to study known genetic variants associated with pain in individuals with idiopathic scoliosis.

Methods:
We included 1442 individuals with juvenile or adolescent idiopathic scoliosis from Sweden and Denmark. Single nucleotide variants (SNV) genotyping was performed on 37 SNVs. Pain was assessed using 2 questionnaires. The mean pain domain score on the Scoliosis Research Society 22 revised questionnaire (SRS-22r) ranging between 1 (worst) and 5 (best) was dichotomized into a “back pain group” (score <4) and a “no back pain group” (score ≥4). The EuroQol 5-dimensions (EQ-5D) 3 level pain domain was dichotomized into a “no pain group” and a “pain group.” Odds ratios were used to describe the associations.

Results:
Based on the SRS-22r pain domain scores, 456 individuals (32%) reported back pain. Based on the EQ-5D questionnaire, 813 individuals (56%) reported moderate or extreme pain/discomfort. The odds ratio for the associations between the selected genetic variants and back pain or pain in general as measured with SRS-22r and EQ-5D-3L ranged between 0.88 to 1.17 and 0.86 to 1.16, with P-values ranging between 0.08 to 0.99 and 0.08 to 0.95.

Conclusion:
This study suggests that known genetic variants associated with pain do not play a significant role in the development of pain in individuals with idiopathic scoliosis.
CXCR4—A Potential Novel Theranostic Target in Adrenocortical Carcinoma
Authors: Nazar Amir , Kalshetty Ashwini , Basu Sandip 
Published: December 2024

Abstract
Adrenocortical carcinoma (ACC) is a highly aggressive malignancy with high recurrence rates and poor prognosis. There are only limited treatment options with poor response rates. ACCs are documented to express chemokine receptor CXCR4, which can be visualized using 68Ga-pentixafor PET/CT. We herein present a case of ACC with local recurrence and peritoneal metastases, which was well visualized on FDG and pentixafor PET/CT. The high expression of CXCR4 offers the potential of theranostics and targeted endoradiotherapy in patients with metastatic ACC. We also observed thymic rebound to illustrate mild to moderate grade uptake of 68Ga-pentixafor in this patient.
A photoswitchable HaloTag for spatiotemporal control of fluorescence in living cells
Authors: Walterspiel Franziska , Ugarte-Uribe Begoña , Weidenhausen Jonas , Dimitriadi Anna , Khan Arif Ul Maula , Müller Christoph W. , Deo Claire 
Published: December 2024

ABSTRACTPhotosensitive fluorophores, which emission can be controlled using light, are essential for advanced biological imaging, enabling precise spatiotemporal tracking of molecular features, and facilitating super-resolution microscopy techniques. While irreversibly photoactivatable fluorophores are well established, reversible reporters which can be re-activated multiple times remain scarce, and only few have been applied in living cells using generalizable protein labelling methods. To address these limitations, we introduce chemigenetic photoswitchable fluorophores, leveraging the self-labelling HaloTag protein with fluorogenic rhodamine dye ligands. By incorporating a light-responsive protein domain into HaloTag, we engineer a tunable, photoswitchable HaloTag (psHaloTag), which can reversibly modulate the fluorescence of a bound dye-ligand via a light-induced conformational change. Our best performing psHaloTag variants show high performancein vitroand in living cells, with large, reversible, far-red fluorescence turn-on upon 450 nm illumination across various biomolecular targets. Together, this work establishes the chemigenetic approach as a versatile platform for the design of photoswitchable reporters, tunable through both genetic and synthetic modifications, with promising applications for dynamic imaging.
Ancestry-linked stromal variations impact breast epithelial cell invasion
Authors: Schmidtmann Madison , Elliott Victoria , Clancy James , Nakshatri Harikrishna , D’Souza-Schorey Crislyn 
Published: December 2024

AbstractBreast cancer is a significant health challenge worldwide, and disproportionately affects women of African ancestry (AA) who experience higher mortality rates relative to other racial/ethnic groups. Several studies have pointed to biological factors that affect breast cancer outcomes. A recently discovered stromal cell population that expressesPROCR,ZEB1 andPDGFRα (PZP cells) was found to be enriched in normal healthy breast tissue from AA donors, and only in tumor adjacent tissues from donors of European ancestry (EA). Here, we investigated the effect of PZP cells on the acquisition of tumorigenic phenotypes in AA and EA human breast epithelial cells to determine its contribution to the biological basis of outcome disparities. Using 3D cell models of tumor invasion, we find that PZP cells confer invasive capacity to epithelial cells independent of genetic ancestry, wherein cells exhibit leader-follower behaviors during extracellular matrix invasion. Enhanced epithelial invasion stems from a combination of AKT activation and fibronectin deposition by the PZP cells. Although activation of AKT in epithelial cells alone is insufficient to induce invasive behaviors, blocking AKT activation markedly reduces invasive capacity. These findings point to the germaneness of differences in breast biology and the multi-faceted roles and enrichment in PZP cells in AA breast tissue, in furthering current understanding of the molecular basis for worse prognosis for patients of African descent.
H3K4-H3K9 Histone Methylation Patterns and Oncofetal Developmental Networks as Drivers of Cell Fate Decisions in Pediatric High-Grade Gliomas
Authors: Uthamacumaran Abicumaran 
Published: December 2024

ABSTRACTThis study employs systems medicine approaches, including complex networks and machine learning-driven discovery, to identify key biomarkers governing phenotypic plasticity in pediatric high-grade gliomas (pHGGs), namely, IDHWT glioblastoma and H3K27M diffuse intrinsic pontine glioma (DIPG). By integrating single-cell transcriptomics and histone mass cytometry data, we conceptualize these aggressive tumors as complex adaptive ecosystems driven by hijacked oncofetal developmental programs and pathological attractor dynamics. Our analysis predicts lineage-plasticity markers, including KDM5B (JARID1B), ARID5B, GATA2/6, WNT, TGFβ, NOTCH, CAMK2D, ATF3, DOCK7, FOXO1/3, FOXA2, ASCL4, PRDM9, METTL5/8, RAP1B, CD99, RLIM, TERF1, and LAPTM5, as drivers of cell fate cybernetics. Further, we identified endogenous bioelectric signatures, including GRIK3, GRIN3, SLC5A9, NKAIN4, and KCNJ4/6, as potential reprogramming targets. Additionally, we validate previously discovered plasticity genes such as PDGFRA, EGFR targets, OLIG1/2, FXYD5/6, MTSS1, SEZ6L, MTRN2L1, and SOX11, confirming the robustness of our complex systems approaches. This systems oncology framework offers promising avenues for precision medicine, optimizing patient outcomes by guiding combination therapies informed by single-cell multi-omics and targeting pHGG phenotypic plasticity as therapeutic vulnerabilities. Further, our findings suggest the epigenetic reprogrammability of tumor phenotypic plasticity (i.e., transition therapy) and maladaptive behaviors in pHGG ecosystems toward stable, transdifferentiated states.
Enzyme-independent functions of HDAC3 in the adult heart
Authors: Qian Sichong , Zhang Chen , Li Wenbo , Song Shiyang , Lin Guanqiao , Cheng Zixiu , Zhou Wenjun , Yin Huiqi , Li Haiyang , Shen Hu-Ying , Sun Zheng 
Published: December 2024

ABSTRACTThe cardioprotective effects of histone deacetylase (HDAC) inhibitors (HDIs) are at odds with the deleterious effects of HDAC depletion. Here, we use HDAC3 as a prototype HDAC to address this contradiction. We show that adult-onset cardiac-specific depletion of HDAC3 in mice causes cardiac hypertrophy and contractile dysfunction on a high-fat diet (HFD), excluding developmental disruption as a major reason for the contradiction. Genetically abolishing HDAC3 enzymatic activity without affecting its protein level does not cause cardiac dysfunction on HFD. HDAC3 depletion causes robust downregulation of lipid oxidation/bioenergetic genes and upregulation of antioxidant/anti-apoptotic genes. In contrast, HDAC3 enzyme activity abolishment causes much milder changes in far fewer genes. The abnormal gene expression is cardiomyocyte-autonomous and can be rescued by an enzyme-dead HDAC3 mutant but not by an HDAC3 mutant (Δ33-70) that lacks interaction with the nuclear-envelope protein lamina-associated polypeptide 2β (LAP2β). Tethering LAP2β to the HDAC3 Δ33-70 mutant restored its ability to rescue gene expression. Finally, HDAC3 depletion, not loss of HDAC3 enzymatic activity, exacerbates cardiac contractile functions upon aortic constriction. These results suggest that the cardiac function of HDAC3 in adults is not attributable to its enzyme activity, which has implications for understanding the cardioprotective effects of HDIs.