bluuebunny/crossref_metadata_2025_split huggingface dataset:
Clinical significance of antibodies to antigens in the Raph, John Milton Hagen, I, Globoside, Gill, Rh-associated glycoprotein, FORS, JR, LAN, Vel, CD59, and Augustine blood group systems
Authors: Moghaddam M. , Naghi A.A. 
Published: April 2021

Abstract
This article reviews information on the clinical significance of antibodies to antigens in the Raph, John Milton Hagen, I, Globoside, Gill, Rh-associated glycoprotein, FORS, JR, LAN, Vel, CD59, and Augustine blood group systems. Antibodies to many of the antigens in these groups are rarely encountered because of the high prevalence of the associated antigens in most populations. For many of these antibodies, the clinical significance—that is, the potential to cause reduced survival of transfused antigen-positive red blood cells or a transfusion reaction (e.g., anti-P, anti-Jra, and anti-Lan), and/or hemolytic disease of the fetus and newborn (e.g., anti-RHAG4 and anti-Vel)—has been documented. For other antibodies, their prevalence is so rare that information on the clinical significance of their antibodies is not available (e.g., anti-FORS1).
Immunohematology

2018;34:85–90.
Virulence of Steinernema ceratophorum against different pest insects and their potential for in vivo and in vitro culture
Authors: Yan Xun , Chen Guimei , Chen Yuqing , Sun Bingjiao , Gu Xinghui , Ruan Weibing , Han Richou 
Published: April 2021

Abstract
Entomopathogenic nematodes (EPN) are biological control agents used to control different insect pests. Indigenous EPN resources are important for the successful control of endemic pests. A native EPN isolate collected in Yunnan, China that showed high virulence against Spodoptera litura was identified and studied for virulence and production potential. The EPN isolate was identified as Steinernema ceratophorum and showed high virulence against different larval stages of Spodoptera exigua, Ostrinia nubilalis, Helicoverpa armigera, and the 2nd instar larvae of Spodoptera frugiperda when applied at 50 IJ/larva under in vitro conditions. In vivo culture of the S. ceratophorum isolate showed that Galleria mellonella larvae were the most suitable host compared to Tenebrio molitor and S. litura. The highest yield in in vivo culture was 1.15 × 105 IJ/larva when cultured in G. mellonella with inoculum concentration of 50 IJ/larva at 25°C. For in vitro solid culture, the highest yield for S. ceratophorum was 2.09 × 106 IJ/g when cultured at optimal temperature of 25°C. Nematodes from the in vivo and in vitro culture, both retained their virulence against the G. mellonella larvae. The S. ceratophorum showed high potential to be mass produced, which will enable the commercial use of this EPN isolate. This is the first attempt to mass culture the S. ceratophorum species. The study explores the usage of S. ceratophorum and provides basis for the commercialization of the EPN species.
HABITAT PERKOTAAN DI KOTA KUPANG
Authors: Liem Yoseph , Mahagarmitha Rilia Rigina 
Published: April 2021

Title: Urban Habitat in Kupang City
The issue of urban space clash in Indonesia is not only in the level of space occupation but also in the loss of habitat-driving force in the city. It is also happening in the city of Kupang as capital of East Nusa Tenggara Province, and also as the border region with the state of Timor Leste. The city of Kupang developing at this time, continues to carry out the growth to the coastal areas . It is in fact contradictory to the regulations that have been made before. The area in this case study of the research is Pasir Panjang Beach - Kelapa Lima, where it is locate in previously a habitat for fishermen colonies in the coastal area of Kupang City. Next to this location is a public space. This study aims to determine the conflict of interest in the space utilization in the city of Kupang. The descriptive research methods will be used, through literature studies, Kupang City government documents, observations, and interviews with local communities. Based on the results of the analysis, there are deviations in the function of urban space, which initially is a public space, then turned into a commercial area. Thus, in this study, it can be concluded that urban space can be assessed in terms of its function and role within the city in reallity can be influenced through the process of adaptation, negotiation, and power play. Changes in function of the urban space can cause various kinds of urban problems, including social issues, the environment, and unbalanced city growth.
PENDEKATAN NARRATIVE DALAM PERANCANGAN KONEKTIVITAS RUANG SEMPADAN DI JALAN TAMAN KEMANG
Authors: Pantan Robert Christopher , Dewi Julia , Yanuar Andreas 
Published: April 2021

Title: Narrative Approach in the Design of Integrated Space Connectivity in Taman Kemang
As a commercial area the Taman Kemang street has certain activities based on its functions such as parking ,working, selling (street vendors), eating and relaxing, people who just walk to Lippo Mall Kemang and activities from online transportation to deliver and pick up. However, as a commercial area, the Taman Kemang street does not have the balance of the daily activities as the city space. This is because different activities are defined by time from morning to night and weekdays and weekends. This is because the bordering space that are formed are utilized as parking lots and selling places of the street vendors which it should be utilized more as public spaces. Therefore, in this research, the author uses a narrative approach as an analysis process towards the design stage through the stages of the story - narrative - narration as a problem-solving solution. The process of analysis is based on activities on the Taman Kemang street through the motives of community activities that form events in a certain time. So that in the narrative stage a story concept is formed which is how to revive the bordering space of the daily narrative formed on the Taman Kemang street. Then supported by strategies at the stage of narration through 5 narrative landscape strategies and forming quality space, namely the formation of activity identity, space identity and continuity of the results of the integration of bordering space elements.
EFEKTIVITAS KOMUNIKASI PROSES PEMBELAJARAN JARAK JAUH (DARING)
Authors: Widyaningsih Eka 
Published: April 2021

Title: Communication Efficacy During Distance Learning Process (Online)
The Coronavirus pandemic has been forcing us to avoid physical contact in the working field and educational institutions. We have to deal with many ostacles in this new learning and working environment, both technical and non-technical. Unstable internet connectivity and the financial challenge to provide online access for students at home are among the fundamental problems caused by this distance learning process. As a consequence of this study, material delivery and student’s comprehension are at risk. Based on these facts, lecturers and institutions must have proper/ better planning strategies to increase learning outcomes in an academic program as a whole and each course for more aimed results. In an architecture department, studio courses are essential. One strategy example of dealing with today’s situation is a collaboration among 2-3 studio courses on the same level to enhance mutual achievement, creating better synergy between methods. This learning strategy’s objective is to make the learning process delivery more efficient and comprehensible to students.
KOMUNIKASI BARU BIRO ARSITEK DI MASA PANDEMI DALAM PANDANGAN POSTKOLONIALISME HOMI K BHABHA
Authors: Utomo Gayuh Budi , Damayanti Rully , Agustin Dyan 
Published: April 2021

Title: New Communication of The Architecture Firms in Pandemic Era; Following the Homi K Bhabha Post Colonial View
A new order called the new normal is a central issue at this time. The period before the pandemic which became a standard value and became a reference suddenly was not compatible with the arrival of the Covid-19 pandemic outbreak. This is happening in Indonesia and around the world. Everyone is in a pandemic situation for a certain period time and there is no certainty that it will end. This of course also affects how to communicate in all aspects including the architectural bureau. New ways of communicating are carried out at architectural bureaus related to social distancing and physical distancing which are considered as effective prevention methods from the COVID-19 pandemic. The types of communication that have changed are communication with clients, communication with the team and communication with interns. There are significant differences in how to communicate from offline activities to online activities where we can still be connected both ways but not in the same place. This situation is a momentum to free the bonds of limitations that have occurred in terms of communication. We want to interpret this in the postcolonialism approach of Homi K Bhabha which is very relevant to the views of hybridity, ambivalence and the third space as a way of communicating new normal discourses.
Sperm oxidative stress: clinical significance and management
Authors: Gamidov S. I. , Shatylko T. V. , Popova A. Yu. , Gasanov N. G. , Gamidov R. S. 
Published: April 2021

Oxidative stress is one of the leading causes of sperm dysfunction. Excessive amounts of reactive oxygen species can damage sperm membranes and disrupt their DNA integrity, which affects not only the likelihood of getting pregnant naturally, but also the clinical outcomes of assisted reproductive technologies and the risk of miscarriage. Sperm cells are extremely vulnerable to oxidative stress, given the limited functional reserve of their antioxidant systems and the DNA repair apparatus. Lifestyle factors, most of which are modifiable, often trigger generation of reactive oxygen species. Both the lifestyle modification and use of antioxidant dietary supplements are adequate and compatible ways to combat male oxidative stress-associated infertility. The search for other internal and external sources of reactive oxygen species, the identification of the etiology of oxidative stress and treatment of respective diseases are necessary for the successful regulation of redox processes in the male reproductive system in clinical practice, which is required not only to overcome infertility, but also to prevent induced epigenetic disorders in subsequent generations. The article presents the analysis of the molecular mechanisms of male idiopathic infertility. The authors provide an overview of how to prevent oxidative stress as one of the causes of subfebrile fever. The article provides an overview of modern therapeutics, as well as the options for eliminating the consequences of the effect of reactive oxygen species on spermatogenesis and male reproductive system in general.
Features of the course of puerperium in preterm birth with a long anhydrous interval
Authors: Khvorostukhina N. F. , Stepanova N. N. , Novichkov D. A. , Silkina A. A. 
Published: April 2021

Introduction. Preterm birth (PR), complicated by premature rupture of the fetal membranes (PRPO), continues to be the focus of attention of obstetricians-gynecologists and neonatologists, due to high rates of perinatal morbidity and mortality.The purpose of the study: to identify the features of the course of puerperium in preterm birth with a long anhydrous interval.Material and methods. A single-stage cohort comparative study of the course and outcomes of PR complicated by PRPO and an anhydrous interval of more than 12 hours (group 1: n = 153) and spontaneous PR with an anhydrous interval of less than 12 hours (group 2: n = 408) has been conducted. Anamnestic data, peculiarities of pregnancy, childbirth, postpartum period, complications of puerperium and morbidity of newborns have been studied in detail.Results and discussion. Pregnancy in group 1 was significantly more likely to occur against the background of acute respiratory diseases (ARVI - 2 times), colpitis (2.6 times), more often complicated by threatening termination (1.3 times), the development of isthmic-cervical insufficiency (1.3 times) and chorioamnionitis (5.7 times). It has been found that in the group of patients with a long anhydrous interval, the risk of hyperthermia (2 times - up to 49.7%), subinvolution of the uterus (1.5 times - up to 25.5%), late hypotonic bleeding (13 times - up to 6.5%), endometritis (4 times - up to 3.9%) increased significantly in the postpartum period, increasing the probability of generalization of infection and hysterectomy to 1.3%.Conclusion. The results of the study once again have confirmed the negative impact of a long anhydrous interval on the incidence of newborns with PR. In addition, data have been obtained on an increased risk of purulent-septic infection in the puerperal period after PR, complicated by PRPO and a long anhydrous interval. All of the above indicates the need for further research in this area to develop a set of diagnostic and therapeutic measures to predict and prevent complications of puerperium during prolonged pregnancy complicated by PRPO.
New in the differential diagnosis of different variants of thrombotic microangiopathies in obstetrics
Authors: Kirsanova T. V. , Vinogradova M. A. 
Published: April 2021

All variants of thrombotic microangiopathy (TMA) in obstetric practice develop in an avalanche-like manner and require a rapid decision on the choice of treatment tactics. Although all these diseases have similar features, typical of the TMA syndrome as a whole, they are separate diseases with differing etiology and pathogenesis. Because the manifestations of TMA are almost identical in the different diseases, they may all imitate pre-eclampsia and the therapeutic approaches differ, a differential diagnosis is essential and often determines the prognosis.The differential diagnostic search between the different TMA variants that can develop in pregnancy is described, as the prognosis of life often depends on the correct diagnosis. The main differences between pre-eclampsia/HELLP syndrome and life-threatening conditions such as atypical hemolytic-uremic syndrome, thrombotic thrombocytopenic purpura and catastrophic antiphospholipid syndrome are considered. Algorithms of diagnostic search are proposed. Attention is drawn to the fact that existing algorithms for the diagnosis of severe TMA prior to ADAMTS 13 results, based on the severity of thrombocytopenia and azotemia as well as scoring during pregnancy can serve as a guide, but not as a defining feature of the diagnosis.The key to the differential diagnosis is the effect of delivery on the regression of TMA: if hemolysis and thrombocytopenia decrease after delivery, one can speak of ‘pure’ pre-eclampsia (PE) or HELLP-syndrome. If persistent or increasing, thrombotic thrombocytopenic purpura (TTP) and aHUS should be considered. With an ADAMTS 13 level of less than 10%, a diagnosis of TTP is made. With ADAMTS 13 over 10-20%, the patient falls into the «grey zone» and a repeat enzyme test is required. If there are signs of fulminant multiple organ failure, especially in a patient with initial suspicion of APS, CAPS can be suspected. To confirm it, high titres of APA are required. In addition, TMA may cause: sepsis and various urgent obstetric conditions complicated by the development of DIC.
MiR-129-5p Promotes Radio-sensitivity of NSCLC Cells by Targeting SOX4 and RUNX1
Authors: Xue Tongqing , Yin Gang , Yang Weixuan , Chen Xiaoyu , Liu Cheng , Yang Weixi , Zhu Jun 
Published: April 2021

Background:
Dysregulation of microRNAs (miRNAs) figures prominently in the radio-
sensitivity of non-small cell lung cancer (NSCLC). MiR-129-5p can block the development of
a variety of tumors. However, whether miR-129-5p modulates radio-sensitivity of NSCLC cells remains
unknown.

Objective:
This study was aimed to explore the role and the underlying mechanism of miR-129-5p
in the radiosensitivity of NSCLC.

Methods:
Radio-resistant NSCLC cell lines (A549-R and H1299-R) were constructed using A549
and H1299 cells. Quantitative real-time polymerase chain reaction (qRT-PCR) was employed to
quantify miR-129-5p, SRY-box transcription factor 4 (SOX4) mRNA, and RUNX family transcription
factor 1 (RUNX1) mRNA expression levels. Cell apoptosis and cell cycle were detected by
flow cytometry. Cell counting kit-8 (CCK-8) assay and colony formation experiments were used to
measure cell proliferation. γ-H2AX was examined by Western blot to confirm DNA injury. Dual-
luciferase reporter experiments were applied to analyze the interactions among miR-129-5p,
RUNX1, and SOX4.

Results:
In A549-R and H1299-R cells, compared with the wild-type cell lines, miR-129-5p expression
was remarkably reduced while SOX4 and RUNX1 expressions were increased. The transfection
of miR-129-5p into NSCLC cell lines markedly induced cell apoptosis, DNA injury, cell cycle
arrest, and inhibited cell proliferation and colony formation. RUNX1 and SOX4 were validated as
target genes of miR-129-5p, and the restoration of RUNX1 or SOX4 could counteract the influence
of miR-129-5p on A549-R cells.

Conclusion:
MiR-129-5p sensitizes A549-R and H1299-R cells to radiation by targeting RUNX1
and SOX4.
Fear of COVID-19 among Peruvian People Living in Disadvantaged Communities: A Cross-Sectional Study
Authors: Sotomayor-Beltran Carlos , Matta-Solis Hernan , Perez-Siguas Rosa , Matta-Solis Eduardo , Matta-Zamudio Lourdes 
Published: April 2021

Background:
The COVID-19 crisis is fuelling a state of fear among the human population at global level. Especially, those living in informal settlements and slums worldwide have been profoundly impacted by this pandemic. Individuals living in these places are already leading underprivileged lives. Thus, the economic and mental health problems caused by the COVID-19 crisis have further exacerbated their living standards, which has resulted, for instance, in tragedies such as suicides.

Objective:
In this study, we have sought to identify those individuals most at risk of displaying high levels of fear of COVID-19 in an informal settlement located in the capital city of Peru.

Methods:
A questionnaire was administered to 449 inhabitants living in the Carmen Alto informal settlement. The questionnaire was made up of two parts: the first one inquired about demographic data and the second part consisted of the Fear of COVID-19 Scale.

Results:
The demographic variables of age, gender, marital status, educational level, occupation, whether a relative from the household was infected with COVID-19, and whether one of them died of this showed significant differences. It could be observed as well that the groups of females, stable workers, unemployed and those having completed a workforce education are at higher odds of displaying high levels of fear of COVID-19. As expected, the groups that had either a relative infected with COVID-19 or a relative death by this had the highest levels of fear towards the virus.

Conclusion:
The female participants are more likely to display higher levels of fear of COVID-19 due to the terrible effect that unfavorable events have on them. In the cases of the unemployed and stable workers, their proneness to show high levels of fear towards the virus is because they have lost their incomes, due to the loss of their jobs, and because of fear of infection, respectively. Hence, we hope that this work serves Peruvian (and other) health authorities to develop strategies that help individuals living in informal settlements and are in urgent need of mitigating mental health problems.
Socio-Demographic and Environmental Determinants of Adverse Childhood Experience among School-Going Adolescents in Jimma town, Jimma, Southwest Ethiopia
Authors: Tsehay Mekonnen , Necho Mogesie , Belete Asmare , Belayneh Zelalem 
Published: April 2021

Introduction:
Adverse childhood experience in adolescents is a global public health concern. Several risk factors have been identified so far across different parts of the world. However, there is a paucity of data in Ethiopia.

Objective:
To determine the prevalence and associated factors of Adverse Childhood Experience among school-going adolescents retrospectively.

Methods:
A cross-sectional school-based study was employed. Participants were selected using a multistage sampling technique. Adverse Childhood Experience Questionnaire Scale for childhood maltreatment. Multiple linear regressions were used to determine the association between Adverse Childhood Experience & associated factors.

Results:
A total of 546 adolescents participated in the study with a response rate of 86%. The mean (±SD) age of participants was 16.83 ± 1.26 years. The majority (442 (81%)) were studying in public schools and 104 (19%) in private schools. Results show that 51.1% reported at least one, and respondents most often faced two ACEs (29.4%), and as many as 10.4% reported at least four or more ACEs. According to the three categories of adverse childhood experience (ACE), from 329 female adolescents, 144(43.8%) had been abused physically, verbally, or sexually, 65(17.8%) had been neglected, and 169(51.1%) had been household dysfunctional. And also, from 217 male adolescents, 93(42.9%) had been abused physically, verbally, or sexually, 49(22%) had neglected, and 117(53.9%) had household dysfunction. Gender, social support, residence, educational status of parent, and having one or more chronic medical, mental, or neurological disorders were significantly associated with Adverse Childhood Experiences.

Conclusion:
Results of the study show that there was a significant prevalence of Adverse Childhood Experiences. Adverse Childhood Experiences have a tremendous impact on future health, well-being, and opportunity. Reduction and prevention of Adverse Childhood Experiences are mandatory for all children to reach their full potential. Those who are already exposed to Adverse Childhood Experiences also need social support, help them manage their experiences, and lead meaningful lives.