bluuebunny/crossref_metadata_2025_split huggingface dataset:
Expression patterns of signalling molecules and transcription factors in the early rabbit embryo and their significance for modelling amniote axis formation
Authors: Plöger Ruben , Viebahn Christoph 
Published: June 2021

AbstractThe anterior-posterior axis is a central element of the body plan and, during amniote gastrulation, forms through several transient domains with specific morphogenetic activities. In the chick, experimentally proven activity of signalling molecules and transcription factors lead to the concept of a ‘global positioning system’ for initial axis formation whereas in the (mammotypical) rabbit embryo, a series of morphological or molecular domains are part of a putative ‘three-anchor-point model’. Because circular expression patterns of genes involved in axis formation exist in both amniote groups prior to, and during, gastrulation and may thus be suited to reconcile these models, the expression patterns of selected genes known in the chick, namely the ones coding for the transcription factors eomes and tbx6, the signalling molecule wnt3 and the wnt inhibitor pkdcc, were analysed in the rabbit embryonic disc using in situ hybridisation and placing emphasis on their germ layer location. Peripheral wnt3 and eomes expression in all layers is found initially to be complementary to central pkdcc expression in the hypoblast during early axis formation. Pkdcc then appears — together with a posterior-anterior gradient in wnt3 and eomes domains — in the epiblast posteriorly before the emerging primitive streak is marked by pkdcc and tbx6 at its anterior and posterior extremities, respectively. Conserved circular expression patterns deduced from some of this data may point to shared mechanisms in amniote axis formation while the reshaping of localised gene expression patterns is discussed as part of the ‘three-anchor-point model’ for establishing the mammalian body plan.
Diagnosis and treatment of Chiari malformation type 1 in children: the International Consensus Document
Authors: Massimi Luca , Peretta Paola , Erbetta Alessandra , Solari Alessandra , Farinotti Mariangela , Ciaramitaro Palma , Saletti Veronica , Caldarelli Massimo , Canheu Alexandre Casagrande , Celada Carlo , Chiapparini Luisa , Chieffo Daniela , Cinalli Giuseppe , Di Rocco Federico , Furlanetto Marika , Giordano Flavio , Jallo George , James Syril , Lanteri Paola , Lemarchand Christian , Messing-Jünger Martina , Parazzini Cecilia , Paternoster Giovanna , Piatelli Gianluca , Poca Maria. A. , Prabahkar Prab , Ricci Federica , Righini Andrea , Sala Francesco , Sahuquillo Juan , Stoodley Marcus , Talamonti Giuseppe , Thompson Dominic , Triulzi Fabio , Zucchelli Mino , Valentini Laura ,   International Experts - Jury of the Chiari & Syringomyelia Consensus Conference, “Milan, November 11–13, 2019”
Published: June 2021

Abstract
Background
Chiari malformation type 1 (CM1) is a rare condition where agreed classification and treatment are still missing. The goal of this study is to achieve a consensus on the diagnosis and treatment of CM1 in children.

Methods
A multidisciplinary panel formulated 57 provisional statements based on a review of the literature. Thirty-four international experts (IE) participated in a Delphi study by independently rating each statement on a 4-point Likert scale (“strongly disagree,” “disagree,” “agree,” “strongly agree”). Statements that were endorsed (“agree” or “strongly agree”) by < 75% of raters were re-formulated, or new statements were added, and another Delphi round followed (up to a maximum of three).

Results
Thirty-five IE were contacted and 34 agreed to participate. A consensus was reached on 30/57 statements (52.6%) after round 1. Three statements were added, and one removed. After round 2, agreement was reached on 56/59 statements (94.9%). Finally, after round 3, which took place during the 2019 Chiari Consensus Conference (Milan, Italy), agreement was reached on 58/59 statements (98.3%) about four main sections (Definition and Classification, Planning, Surgery, Isolated Syringomyelia). Only one statement did not gain a consensus, which is the “definition of radiological failure 24 month post-surgery.”

Conclusions
The consensus document consists of 58 statements (24 on diagnosis, 34 on treatment), serving clinicians and researchers following children with CM1. There is a clear need for establishing an international network and registry and to promote collaborative studies to increase the evidence base and optimize the long-term care of this patient population.
Sjögren’s syndrome: a systemic autoimmune disease
Authors: Negrini Simone , Emmi Giacomo , Greco Monica , Borro Matteo , Sardanelli Federica , Murdaca Giuseppe , Indiveri Francesco , Puppo Francesco 
Published: June 2021

AbstractSjögren’s syndrome is a chronic autoimmune disease characterized by ocular and oral dryness resulting from lacrimal and salivary gland dysfunction. Besides, a variety of systemic manifestations may occur, involving virtually any organ system. As a result, the disease is characterized by pleomorphic clinical manifestations whose characteristics and severity may vary greatly from one patient to another. Sjögren’s syndrome can be defined as primary or secondary, depending on whether it occurs alone or in association with other systemic autoimmune diseases, respectively. The pathogenesis of Sjögren’s syndrome is still elusive, nevertheless, different, not mutually exclusive, models involving genetic and environmental factors have been proposed to explain its development. Anyhow, the emergence of aberrant autoreactive B-lymphocytes, conducting to autoantibody production and immune complex formation, seems to be crucial in the development of the disease. The diagnosis of Sjögren’s syndrome is based on characteristic clinical signs and symptoms, as well as on specific tests including salivary gland histopathology and autoantibodies. Recently, new classification criteria and disease activity scores have been developed primarily for research purposes and they can also be useful tools in everyday clinical practice. Treatment of Sjögren’s syndrome ranges from local and symptomatic therapies aimed to control dryness to systemic medications, including disease-modifying agents and biological drugs. The objective of this review paper is to summarize the recent literature on Sjögren’s syndrome, starting from its pathogenesis to current therapeutic options.
Stories Told Together: Male Narratives of Non-Monogamous Bi+ and Heterosexual Men
Authors: Castro Aurelio 
Published: June 2021

AbstractThe stories we tell about our identities and sexual orientations shape how we perform gendered scripts and negotiate relationships with significant others. Previous literature inquired the styles and outcomes of consensual non-monogamous (CNM) relationships, but more research is need on how CNM men resist or abide to hegemonic models of masculinity. To understand how constructions of masculinity and conceptualizations of sexual orientation are embedded in CNMs, the study analysed the stories of non-monogamous Bi+ and heterosexual men. Following a critical narrative approach, the study inquired the diverse conceptualizations of masculinity, sexual orientation and relationship practices in the narratives of 20 non-monogamous Bi+ and heterosexual identified men. The semi-structured in-depth narrative interviews (105 min on average) were analyzed via Nvivo 12 and explored their stories of desire and the sense-making process of being sexually oriented to one or more genders and to one or more partner/s. Engaging in non-monogamy was signified as a relevant insight from their personal stories and/or from adopting new concepts of desire beyond the “love as a zero-sum game.” The latter theme was also shared by many heterosexual participants that, when negotiating a non-monogamous agreement, signified their attractions to more than one person as part of their personal identity. Finally, the paper discusses how non-monogamous spaces can offer a positive and safe space for bisexuals/Bi+ people to explore and reaffirm their identities, constantly challenged by biphobia, invisibility, and erasure. Experiences and stories of Italian cisgender Bi+ and heterosexual men cannot be generalized to the whole spectrum of masculinities within CNM spaces, and the study lacks how other gendered and sexual subjectivities construct masculinity. Diverse stories and construction of sexuality and gender can lead to similar relationship preferences and understanding how we signify them can greatly improve our understanding of intimacies.
Soil age and soil organic carbon content shape biochemical responses to multiple freeze–thaw events in soils along a postmining agricultural chronosequence
Authors: Rosinger Christoph , Bonkowski Michael 
Published: June 2021

AbstractFreeze–thaw (FT) events exert a great physiological stress on the soil microbial community and thus significantly impact soil biogeochemical processes. Studies often show ambiguous and contradicting results, because a multitude of environmental factors affect biogeochemical responses to FT. Thus, a better understanding of the factors driving and regulating microbial responses to FT events is required. Soil chronosequences allow more focused comparisons among soils with initially similar start conditions. We therefore exposed four soils with contrasting organic carbon contents and opposing soil age (i.e., years after restoration) from a postmining agricultural chronosequence to three consecutive FT events and evaluated soil biochgeoemical responses after thawing. The major microbial biomass carbon losses occurred after the first FT event, while microbial biomass N decreased more steadily with subsequent FT cycles. This led to an immediate and lasting decoupling of microbial biomass carbon:nitrogen stoichiometry. After the first FT event, basal respiration and the metabolic quotient (i.e., respiration per microbial biomass unit) were above pre-freezing values and thereafter decreased with subsequent FT cycles, demonstrating initially high dissimilatory carbon losses and less and less microbial metabolic activity with each iterative FT cycle. As a consequence, dissolved organic carbon and total dissolved nitrogen increased in soil solution after the first FT event, while a substantial part of the liberated nitrogen was likely lost through gaseous emissions. Overall, high-carbon soils were more vulnerable to microbial biomass losses than low-carbon soils. Surprisingly, soil age explained more variation in soil chemical and microbial responses than soil organic carbon content. Further studies are needed to dissect the factors associated with soil age and its influence on soil biochemical responses to FT events.
Comparison of two targeted ultra-deep sequencing technologies for analysis of plasma circulating tumour DNA in endocrine-therapy-resistant breast cancer patients
Authors: Nteliopoulos Georgios , Page Karen , Hills Allison , Howarth Karen , Emmett Warren , Green Emma , Martinson Luke J. , Fernadez-Garcia Daniel , Hastings Robert , Guttery David S. , Kenny Laura , Stebbing Justin , Cleator Susan , Rehman Farah , Gleason Kelly L. T. , Sanela Andrijac , Ion Charlotte , Rushton Amelia J. , Rosenfeld Nitzan , Coombes R. Charles , Shaw Jacqueline A. 
Published: June 2021

AbstractPurposeThere is growing interest in the application of circulating tumour DNA (ctDNA) as a sensitive tool for monitoring tumour evolution and guiding targeted therapy in patients with cancer. However, robust comparisons of different platform technologies are still required. Here we compared the InVisionSeq™ ctDNA Assay with the Oncomine™ Breast cfDNA Assay to assess their concordance and feasibility for the detection of mutations in plasma at low (< 0.5%) variant allele fraction (VAF).MethodsNinety-six plasma samples from 50 patients with estrogen receptor (ER)-positive metastatic breast cancer (mBC) were profiled using the InVision Assay. Results were compared to the Oncomine assay in 30 samples from 26 patients, where there was sufficient material and variants were covered by both assays. Longitudinal samples were analysed for 8 patients with endocrine resistance.ResultsWe detected alterations in 59/96 samples from 34/50 patients analysed with the InVision assay, most frequently affectingESR1, PIK3CAandTP53. Complete or partial concordance was found in 28/30 samples analysed by both assays, and VAF values were highly correlated. Excellent concordance was found for most genes, and most discordant calls occurred at VAF < 1%. In longitudinal samples from progressing patients with endocrine resistance, we detected consistent alterations in sequential samples, most commonly inESR1andPIK3CA.ConclusionThis study shows that both ultra-deep next-generation sequencing (NGS) technologies can detect genomic alternations even at low VAFs in plasma samples of mBC patients. The strong agreement of the technologies indicates sufficient reproducibility for clinical use as prognosic and predictive biomarker.
Anger Responses in Adolescents: Relationship with Punishment and Reward Sensitivity
Authors: Kreuze L. J. , de Jong P. J. , Bennik E. C. , Nauta M. H. 
Published: June 2021

AbstractA substantial proportion of youth with anxiety disorders shows comorbid behavioral (anger) problems. Such comorbid profile is associated with low treatment effectiveness and negative (longterm) outcomes. This study was therefore designed to examine trait factors that may promote anger responding in adolescents. By presenting participants (N = 158, mean age = 15.7, 56% female) with a series of common anger-eliciting situations, we tested whether high reward sensitivity would be associated with anger via perceived non-reward, and high punishment sensitivity via perceived threat. In line with the hypotheses, an indirect effect of reward sensitivity on anger was found via perceived non-reward, and an indirect effect of punishment sensitivity on anger via perceived threat. The latter association also had an indirect effect via perceived non-reward. High punishment and reward sensitivity may thus set adolescents at risk for developing (comorbid) anger problems via heightened threat and non-reward perceptions.
Guidelines and pitfalls of refraction microtremor surveys
Authors: Louie John N. , Pancha Aasha , Kissane B. 
Published: June 2021

AbstractThe geotechnical industry has widely adopted the refraction microtremor shear-wave velocity measurement technique, which is accepted by building authorities for evaluation of seismic site class around the world. Clark County and the City of Henderson, Nevada, populated their Earthquake Parcel Map with over 10,000 site measurements for building code enforcement, made over a 3-year period. 2D refraction microtremor analysis now allows engineers to image lateral shear-wave velocity variations and do passive subsurface imaging. Along with experience at a basic level, the ability to identify the “no energy area” and the “minimum-velocity envelope” on the slowness-frequency (p-f) image helps practitioners to assess the quality of their ReMi data and analysis. Guides for grading (p-f) image quality, and for estimating depth sensitivity, velocity-depth tradeoffs, and depth and velocity resolution also assist practitioners in deciding whether their refraction microtremor data will meet their investigation objectives. Commercial refraction microtremor surveys use linear arrays, and a new criterion of 2.2% minimum microtremor energy in the array direction allows users to assess the likelihood of correct results. Unfortunately, any useful and popular measurement technique can be abused. Practitioners must follow correct data collection, analysis, interpretation, and measurement procedures, or the results cannot be labeled “refraction microtremor” or “ReMi” results. We present some of the common mistakes and provide solutions with the objective of establishing a “best practices” template for getting consistent, reliable models from refraction microtremor measurements.
Leveraging Transmedia storytelling to engage tourists in the understanding of the destination’s local heritage
Authors: Dionisio Mara , Nisi Valentina 
Published: June 2021

AbstractTransmedia Stories are becoming an increasingly important technique for the tourism industry. They are successful tools to engage, inspire and gather audiences online and offline. In this article, we describe the design, implementation and evaluation of Fragments of Laura, a bespoke Transmedia Storytelling (TS) experience designed to involve visitors in developing knowledge and awareness about the cultural and natural heritage of Madeira Island. Fragments of Laura (FoL) is composed of two interconnected components: a Location-Aware Multimedia Story, and a Hypermedia Platform populated with locally collected testimonies and interviews. Results from the extensive evaluation of Fragments of Laura, highlights the potential of interactive multimedia, TS in particular, in engaging tourists with the destination values and community. Our contribution is twofold, on one hand we extend on the state of the art of multimedia interactive storytelling, with the description of the Fragments of Laura TS artifact. On the other hand, results from FoL evaluation highlight how the artifact impacts on the tourism experience its implications for the design of future tourism driven TS experiences.
The dynamics of gene transcription with a periodic synthesis rate
Authors: Sun Qiwen , Jiao Feng , Yu Jianshe 
Published: June 2021

AbstractThe periodic transcription output is ubiquitously observed in an isogenic cell population. To understand mechanisms of cyclic behavior in transcription, we extend the gene activation process in the two-state model by assuming that the synthesis rate is periodic. We derive the analytical forms of the mean transcript level and the noise. The limits of them indicate that the mean level and the noise display periodic behaviors. Numerical examples strongly suggest that the transcription system with a periodic synthesis rate generates more noise than that with a constant rate but maintains transcription homeostasis in each period. It is also suggested that if the periodicity is not considered, the calculated noise may be greater than the real value.
Adult body growth and reproductive investment vary markedly within and across Atlantic and Pacific herring: a meta-analysis and review of 26 stocks
Authors: dos Santos Schmidt Thassya C. , Hay Doug E. , Sundby Svein , Devine Jennifer A. , Óskarsson Guðmundur J. , Slotte Aril , Wuenschel Mark J. , Lajus Dmitry , Johannessen Arne , van Damme Cindy J. G. , Bucholtz Rikke H. , Kjesbu Olav S. 
Published: June 2021

AbstractLife-history traits of Pacific (Clupea pallasii) and Atlantic (Clupea harengus) herring, comprising both local and oceanic stocks subdivided into summer-autumn and spring spawners, were extensively reviewed. The main parameters investigated were body growth, condition, and reproductive investment. Body size of Pacific herring increased with increasing latitude. This pattern was inconsistent for Atlantic herring. Pacific and local Norwegian herring showed comparable body conditions, whereas oceanic Atlantic herring generally appeared stouter. Among Atlantic herring, summer and autumn spawners produced many small eggs compared to spring spawners, which had fewer but larger eggs—findings agreeing with statements given several decades ago. The 26 herring stocks we analysed, when combined across distant waters, showed clear evidence of a trade-off between fecundity and egg size. The size-specific individual variation, often ignored, was substantial. Additional information on biometrics clarified that oceanic stocks were generally larger and had longer life spans than local herring stocks, probably related to their longer feeding migrations. Body condition was only weakly, positively related to assumingly in situ annual temperatures (0–30 m depth). Contrarily, body growth (cm × y−1), taken as an integrator of ambient environmental conditions, closely reflected the extent of investment in reproduction. Overall, Pacific and local Norwegian herring tended to cluster based on morphometric and reproductive features, whereas oceanic Atlantic herring clustered separately. Our work underlines that herring stocks are uniquely adapted to their habitats in terms of trade-offs between fecundity and egg size whereas reproductive investment mimics the productivity of the water in question.
A Life-Course View on Ageing Consumers: Old-Age Trajectories and Gender Differences
Authors: Pannhorst Matthias , Dost Florian 
Published: June 2021

AbstractThis study presents a dynamic, model-based view of consumers’ ageing developments, focused on gender differences, to uncover the pathways and socioeconomic transitions that female and male consumers take through old age. The analysis of longitudinal survey data spanning 15 years uses a latent Markov dynamic cluster model with transitions over time. The resulting life courses allow an exploration of lifestyle-related changes in multiple consumer well-being variables beyond age 50. Substantial well-being differences appear in the ageing paths of men and women. In both cases, a dominant chronological sequence through old age is complemented by less common transitions, rarely associated with advanced age. Although the model does not use chronological age as an independent variable, it outperforms purely agebased, or age- cohort-, and period-based models in predicting old-age consumer wellbeing. These results highlight the importance of considering within-cohort diversity when modelling the accompaniments of old age: while some older consumers enjoy active lifestyles, others of similar age succumb to depression and loneliness, rendering age an insufficient predictor of well-being states. In the future, the presented model could be matched with other, even cross-sectional, consumer survey data to help predict various dynamics in the ageing consumer population.
Is Chain Affiliation a Strategic Asset or Constraint in Emerging Economies? Competitive Strategies and Performance in the Russian Hotel Industry
Authors: Karhunen Päivi , Ledyaeva Svetlana 
Published: June 2021

AbstractThe purpose of the paper is to find out how chain affiliation, an important strategic choice and a key determinant for hotel performance, influences the strategy-performance nexus in the emerging economy context. It combines the resource- and institution-based views to investigate how chain affiliation moderates the relationship between competitive strategy and performance. This is done by distinguishing between market-based strategies based on differentiation or cost leadership, and non-market strategies that manifest in institutional advantage. The empirical analysis draws from original survey data on 162 hotels located in the Russian cities Moscow and St. Petersburg. The paper finds that first, in emerging economies the competitive edge of chain-affiliated hotels largely arises from market-based advantage, but only in terms of cost advantage. The finding that differentiation advantage is not an important factor of performance for chain-affiliated hotels suggests that firm-level resources derived from chain affiliation would not transform into competitive advantage in the emerging economy context. Second, it is found that institutional advantage is more important determinant of performance for independent hotels, demonstrating the importance of local knowledge and relationships for firms in emerging economies. This finding also suggests that chain affiliation might be rather a strategic constraint than asset for creating superior performance via institutional advantages in the emerging economy context.