bluuebunny/crossref_metadata_2025_split huggingface dataset:
Emotional modulation of cortical activity during gum-chewing: A functional near-infrared spectroscopy study
Authors: Hasegawa Yoko , Sakuramoto Ayumi , Sakagami Joe , Shiramizu Masako , Suzuki Tatsuya , Tachibana Yoshihisa , Kishimoto Hiromitsu , Ono Yumie , Ono Takahiro 
Published: April 2021

Abstract
Evidence indicates that distinct brain regions are associated with various emotional states. Cortical activity may be modulated by emotional states that are triggered upon chewing with various flavors. We examined cortical activity during chewing with different tastes/odors using multi-channel near-infrared spectroscopy (NIRS).
Thirty-six right-handed subjects participated in a crossover-design trial. Subjects chewed flavorful (palatable) or less flavorful (unpalatable) gum for 5 minutes. During gum-chewing these subjects experienced positive and negative emotions, respectively. Subjects rated the taste/odor/deliciousness of each gum with a visual analog scale. Bilateral hemodynamic responses in the frontal to parietal lobes, bilateral masseter muscle activation, and heart rate were measured during gum-chewing. Data changes during gum-chewing were evaluated.
Subjects’ ratings of the tastes and odors of each gum differed (p<0.001). Hemodynamic response changes were significantly elevated in the bilateral primary sensorimotor cortex during gum-chewing, in comparison to resting. The hemodynamic responses of wide brain regions showed little difference between the gum conditions; however, a difference was detected in the corresponding left frontopolar/dorsolateral prefrontal cortex. Muscle activation and heart rate were not significantly different between the gum conditions.
Differential processing in the left prefrontal cortex might be responsible for emotional states caused by palatable and unpalatable foods.
Ethnoveterinary Knowledge and Practice Applied to Domestic Animals Raised in the Ukraine Colonization Community of Palmital, Paraná State, South Brazil.
Authors: Burei Sarita Teresinha , Santana Diógenes Adriano Duarte , Lopez Bruna Broch , Sotomaior Cristina Santos , Acra Luiz Antônio , Weber Saulo Henrique , Ollhoff Rudiger Daniel 
Published: April 2021

Abstract
Background: A broad spectrum of medicinal plants are used to treat livestock in rural areas of Brazil. The knowledge brought by the first European colonizers merged with traditional indigenous knowledge to treat domestic animals. With the advance of modern medicine, this knowledge is threatened to disappear. Ethnopharmacological studies in northeastern and the Amazon region of Brazil have been conducted, but influence of east Europeans and ethnopharmacological studies in south Brazil are scarce. To find out which plants are used for what purposes by farmers in the rural area of Ukrainian colonization of Palmital, middle west of Paraná state, Brazil. Methods: A semi-structured interview was applied through a non-random sampling with residents with experience in animal husbandry following the “snowball” method. The collected information regarded local plant names, parts of plants used, mode of preparation, route of administration, domestic species and disease treated. The interviewees were encouraged to show the plants used. Photographs were taken and specimen were collected for the family, genus, species identification and storage. The ethnobotanical methods of the informant consensus factor (ICF) and use value (UV) were applied and calculated. Results: 30 women and 20 men reported a total of 45 plant species belonging to 29 families to be used for ethnoveterinary practices. Medicinal plants were used mostly with cattle and goats for 12 different general veterinary uses, with the highest ICF for mastitis and for antiparasitic. Conclusion: A list of native and introduced medicinal plants could be generated. Most plants were used to treat cattle health problems, as the most important livestock species bred in the community. Similar studies with European communities of immigrants in Brazil are unknown and could be a valuable source of information of how cultural blends translate themselves in the use of medicinal plants.
Characterization of the Virome Associated With Haemagogus Mosquitoes in Trinidad, West Indies
Authors: Ali Renee , Jayaraman Jayaraj , Mohammed Azad , Chinnaraja Chinnadurai , Carrington Christine V. F. , Severson David W. , Ramsubhag Adesh 
Published: April 2021

Abstract
Currently, there are increasing concerns about the possibility of a new epidemic due to emerging reports of Mayaro virus (MAYV) fever outbreaks in areas of South and Central America. Haemagogus mosquitoes, the primary sylvan vectors of MAYV are poorly characterized and a better understanding of the mosquito’s viral transmission dynamics and interactions with MAYV and other microorganisms would be important in devising effective control strategies. In this study, a metatranscriptomic based approach was utilized to determine the prevalence of RNA viruses in field-caught mosquitoes morphologically identified as Haemagogus janthinomys from twelve (12) forest locations in Trinidad, West Indies. Known insect specific viruses including the Phasi Chaoren-like and Humaiata- Tubiacanga virus dominated the virome of the mosquitoes throughout sampling locations while other viruses such as the avian leukosis virus, MAYV and several unclassified viruses had a narrower distribution. Additionally, assembled contigs from the Ecclesville location suggests the presence of a unique uncharacterized picorna-like virus. Mapping of RNA sequencing reads to reference partial or complete mitochondrial genomes showed hits against avian and rodent genomes, which putatively add to the growing body of evidence of a potentially wide feeding host-range for the Haemagogus mosquito vector.
Functional Evaluation of KEL As An Oncogenic Gene in The Progression of Acute Erythroleukemia
Authors: Wu Zijuan , Liu Wenjie , Yu Yan , Qiao Chun , Zhu Han , Hong Ming , Zhu Yu , Qian Sixuan , Chen Suning , Wu Depei , Li Jianyong , Jin Hui 
Published: April 2021

Abstract
Background Acute erythroleukemia (AEL) is an infrequent subtype of acute myeloid leukemia (AML) with worse prognosis. Though the last decade has seen major advances in the novel features and genomic landscape in AEL, there is still a lack of specific therapeutic targets and effective treatment approaches for this disease. MethodsTCGA database was used to screen out the oncogene with specifically aberrant expression in AEL. Protein array was performed to explore the activated signaling pathways and targets that undergo phosphorylation modulation. A series of functional analyses in cell lines and mice models were performed to investigate the biological significance and clinical relevance of KEL regulation in AEL. CHIP, EMSA and dual-luciferase activity assay were performed to explore the upstream regulation mechanism of KEL.ResultsIn this study, the results showed that KEL promoted cell proliferation and its downregulation reversed drug resistance in AEL cells to JQ1. Our findings suggested that KEL contributed to gain of H3K27 acetylation and promoted erythroid differentiation induced by GATA1. Additionally, GATA1 and TAL1 as co-Transcription factors (TFs) modulated the expression of KEL. Maintaining cell viability and differentiation, KEL also played parts in the immune evasion of tumor cells. ConclusionsOur work expands the current knowledge regarding molecular mechanisms involved in cancer onset and progression, offering promising therapeutic target to broaden the treatment options.
Change in Employment Status in Survivors With Occupational Cancers: A Case Control Study
Authors: Lim Zhu Wei , Wang Chung-Ching , Wu Wei-Te , Chen Wei-liang 
Published: April 2021

Abstract
Background: The change of employment status in cancer survivors is underinvestigated. The aim of this study is to investigate the change in employment status in survivors with occupational cancers at the time of diagnosis until 2 years. Methods: In this study, we included 382 occupational cancer workers. In these 382 workers, 218 workers were insured. Ninety-nine occupational cancer workers in 2004-2015 were included for the change of employment status and salary buckets. The confirmation of change of employment status was retrieved in the Labor Insurance Database’s employment data. Results: The mean age of cancer workers were 51.38 ± 9.1119 years old and the average salary of industry of NTD 30511.6 ± 3980.6. In our results, most cancer survivors are in 46-55-year-old group (40.7%). Salary adjustment are the most change in the employment status in cancer survivors. Conclusion: Change of employment status in cancer survivors are important to decrease the impact of economic burden on society.
Novel Insight Into The Potential Role of AGPAT Gene Family in Triacylglycerols Synthesis in Buffalo
Authors: Ma Xiaoya , Duan Anqin , Lu Xingrong , Sohel Md Mahmodul Hasan , Abdel-Shafy Hamdy , Amin Ahmed , Liang Shasha , Deng Tingxian 
Published: April 2021

Abstract
Background: Acylglycerophosphate acyltransferases (AGPATs) are enzymes known to act on the second acylation step in the Kennedy (de novo) pathway to catalyze the conversion of lysophosphatidic acid (LPA) to phosphatidic acid (PA). Although AGPATs have been extensively explored by evolution, expression, and functional studies, little is known about the functional characterization of how many members of the AGPAT family are involved in the triacylglycerols (TAG) synthesis. Therefore, the objective of this study was to characterize the potential functional roles of AGPAT family members in the TAG synthesis and growth of mammary epithelial cells using comparative genomic analysis and RNA interference (RNAi) technology.Results: In the present study, a total of 13 AGPAT genes in buffalo were identified using genome-wide analysis. Among them, 12 AGPAT gene pairs were orthologous between buffalo and cattle. The comparative transcriptomic analysis and real-time quantitative reverse transcription PCR (qRT-PCR) further showed that both AGPAT1 and AGPAT6 were highly expressed in milk samples of buffalo and cattle during lactation. Notably, knockdown of AGPAT1 or AGPAT6 significantly decreased the TAG content of buffalo mammary epithelial cells (BuMEC) and bovine mammary epithelial cells (BoMEC) through regulating the mRNA expression levels of several lipogenic genes. Furthermore, the knockdown of AGPAT1 or AGPAT6 inhibited proliferation and promoted apoptosis of BuMECs; the qRT-PCR analysis of marker genes associated with cell proliferation and apoptosis supported the same conclusions. Conclusions: These findings provide new insights about the members of AGPAT family on how they regulate the TAG synthesis and growth of mammary epithelial cells in buffalo. These findings will have important implications for understanding the role of AGPAT gene for buffalo milk production performance.
Circulating miR-375&nbsp;Correlates With Response of Human Individuals to Short-Term Cold Exposure
Authors: Seeliger Claudine , Mengel Laura Aline , Buekens Lise , Mesas-Fernández Alberto , Skurk Thomas , Claussnitzer Melina , Hauner Hans 
Published: April 2021

Abstract
Cold-induced non-shivering thermogenesis (CIT) of the human body is currently discussed as an important contributor to energy metabolism, and miRNAs have recently been reported as key regulators of metabolism. Identifying the link of CIT and circulating microRNAs (miRNAs) in a large cohort of human individuals remains elusive to date. Here, we analyzed a set of 158 miRNAs in the serum of 97 female and 72 male healthy individuals before and after cold exposure (CE). Validating the results of a miRNA array, a significant down-regulation of miR-375 was measured in individuals after CIT (P<0.0001). These changes went along with a significant negative correlation between miR‑375 and the supraclavicular skin temperature (P=0.012). Additionally, the regulation of miR-375 was sex-dependent, with female individuals showing a significantly stronger decrease in expression of miR-375 under CIT compared to males. Such findings were already seen under thermoneutral conditions, where females display significant lower miR-375 expression levels (P=0.015). Besides, the lower miR‑375 expression levels were negatively correlated with the amount of visceral fat tissue in the female cohort (P=0.0002). This study identified miR-375 as a potential new sex-dependent marker for cold-induced thermogenesis.
Search of Official Nationwide Database in Japan for Adverse Events Associated with Disease-modifying Antirheumatic Drug Therapies: Focus on Therapies in Combination with Methotrexate Author
Authors: Inokuma Shigeko 
Published: April 2021

Abstract
Disease-modifying antirheumatic drugs (DMARDs) are essential for rheumatoid arthritis (RA) therapy, and many synthetic and biologic drugs are available. DMARDs are frequently prescribed in combination with methotrexate (MTX), as it is the first-line drug. The adverse events (AEs) associated with DMARDs have sometimes unfavorable outcomes. Major AEs, particularly therapies in combination with methotrexate, were investigated in this study. A search of the website of the Japanese Pharmaceuticals and Medical Devices Agency for AEs associated with therapies with five DMARDs (MTX, tacrolimus, adalimumab, tocilizumab, and abatacept) reported from 2014 to 2016 was performed. The AEs searched included lymphoproliferative disease (LPD), cytopenia, interstitial pneumonia (IP), infectious pneumonia other than Pneumocystis jirovecii pneumonia (PCP) (i-Pn), and PCP. The number of cases of each AE and its ratio to the total number of cases of all AEs associated with each DMARD therapy were examined. Data were compared among AEs and DMARDs. MTX therapy in combination with other DMARDs was examined for rheumatoid arthritis (RA) cases. On the website, a total of 8874 cases were listed as having AEs associated with therapies with the five DMARDs. For MTX therapy, LPD was the most frequent (1438 cases, 36.4% of all AE cases), followed by cytopenia (10.9%), IP (6.2%), i-Pn (4.1%), and PCP (2.6%). Under therapy with any of the other four DMARDs, i-Pn showed the largest number of cases and the highest ratio (4.2–15.3%); other AEs varied in number and ratio. The proportion of use of MTX in combination with the four DMARDs was highest for PCP (67/71, 94.4%), followed by LPD (50/73, 68.5%), cytopenia (48/73, 65.8%), i-Pn (101/173, 58.4%,), and IP (36/80, 45.0%) (Table 1). In total, including cases reported for MTX therapy, 98.2% (1286/1309) of LPD cases, 88.5% (193/218) of cytopenia cases, 79.8% (174/218) of IP cases, 76.4% (233/305) of i-Pn cases, and 97.6% (165/169) of PCP cases had MTX. In conclusion, LPD was by far the most frequent AE associated with MTX therapy. PCP was strongly associated with the use of MTX in combination with another DMARD. For therapy with any of the other four DMARDs, i-Pn showed the highest ratio.
Pharmacist-Led Intervention on the Inappropriate use of Stress Ulcer Prophylaxis Pharmacotherapy in Intensive Care Units: A Systematic Review
Authors: Xu peipei , Yi Qiusha , Wang Cuitong , Zeng Linan , Olsen Keith M. , zhao Rongsheng , Jiang Mingyan , Xu Ting , Zhang Lingli 
Published: April 2021

Abstract
Purpose To determine the effectiveness of pharmacist-led interventions on the inappropriate use of SUP pharmacotherapy in ICUs.Methods A systematic review was performed for relevant studies using searched PubMed, EMBASE (Ovid), the Cochrane Library, Cochrane Central Register of Controlled Trials (CENTRAL), and four Chinese databases including Wanfang, Chinese Biomedical Literature, China National Knowledge Infrastructure (CNKI), and VIP from the establishment of databases to 12 March 2020. We conducted a descriptive analysis of participants, the intervention content and delivery, and the effects on inappropriate medication rates.Results From 529 records, 8 studies from 9 articles were included in the narrative synthesis. All studies were cohort studies, and the NOS quality stars ranged between 5 and 7. Only 1 (12.5%) cohort study was regarded as high quality. The time of appropriateness judgment and the criteria of ‘appropriate’ varied from included studies. Pharmacist interventions mainly included clarifying indications for SUP pharmacotherapy, education and awareness campaign, reviewed patients on SUP pharmacotherapy during rounds, and adjustments of drug use. Five (62.5%) studies found a significant intervention effect during hospitalization, while 2 (25%) studies at ICU transfer and 2 (25%) studies at hospital discharge. Four (50%) studies identified the complications related to SUP pharmacotherapy and found no significant difference. Four (50%) studies declared the pharmacist-led interventions were associated with cost savings.Conclusion Pharmacist-led interventions may be associated with a decrease in inappropriate SUP pharmacotherapy rates during hospitalization, at ICU transfer and hospital discharged. Further research is needed to determine whether the latest guidelines are more suitable for the management of SUP pharmacotherapy and whether pharmacist-led intervention is cost-effective.
Effect of Geography and Captivity on Scat Bacterial Communities in the Imperiled Channel Island Fox
Authors: Adams Nicole E , Becker Madeleine A , Edmands Suzanne 
Published: April 2021

Abstract
Background With developing understanding that host-associated microbiota play significant roles in individual health and fitness, taking an interdisciplinary approach combining microbiome research with conservation science is increasingly favored. Here we establish the scat microbiome of the imperiled Channel Island fox (Urocyon littoralis) and look at the effects of geography and captivity on the variation in bacterial communities. Results Using high throughput 16S rRNA gene amplicon sequencing, we discovered distinct bacterial communities in each island fox subspecies. Weight, timing of the sample collection, and sex contributed to the geographic patterns. We uncovered significant taxonomic differences and an overall decrease in bacterial diversity in captive versus wild foxes. Conclusions Understanding the drivers of microbial variation in this system provides a valuable lens through which to evaluate the health and conservation of these genetically depauperate foxes. The island-specific bacterial community baselines established in this study can make monitoring island fox health easier and understanding the implications of inter-island translocation clearer. The decrease in bacterial diversity within captive foxes could lead to losses in the functional services normally provided by commensal microbes and suggests that zoos and captive breeding programs would benefit from maintaining microbial diversity.
The Process and Effects of Group Sandplay on the Intervention of the Children’s Problem Behaviour From the Perspective of Mentalization
Authors: Zhao Qi Hui , Chen Kan 
Published: April 2021

Abstract
In this study, we explored the interaction feature among children, and between children and therapists in a group sandplay based on mentalization theory. Six children with behavioural problems are selected and divided into two groups for 10 sandplay therapy sessions lasting for 50 minutes each time. All sandplay videos are coded for behaviour according to grounded theory, and the frequency changes of each behaviour in the early, middle and late stages of the intervention process are compared. The results show that group sandplay based on mentalization can reduce the intervention of the children during sandplay. Empathetic consultation relationship in sandplay based on mentalization theory can improve children’s problem behaviors.
Beclin1 controls caspase-4 inflammsome activation and pyroptosis in myocardial reperfusion-induced microvascular injury
Authors: Sun Wenjing , Lu Hongquan , Dong Shujuan , Nan Wang , Zhao Yu , Wang Limiting , Sun Lin , Lu Di 
Published: April 2021

Abstract
Background Myocardial reperfusion injury is often accompanied by cell death and inflammatory reactions. Recently, pyroptosis is gradually recognized as pivotal role in cardiovascular disease. However, little is known about the regulatory role of beclin1 in the control of caspase-4 activation and pyroptosis. The present study confirmed whether beclin1 regulates caspase-4 mediated pyroptosis and thereby protects Cardiac microvascular endothelial cells (CMECs) against injury.Methods TTC and Evan's blue dye, western blot, immunofluorescence and immunohistochemistry staining were performed in wild mice and transgenic mice with overexpression of beclin-1(BECN1-Tg). CMECs were transfected with a beclin1 lentivirus. The cell cytotoxicity was analyzed by LDH-Cytotoxicity Assay Kit. The protein levels of autophagy protein (Beclin1, P62 and LC3II/LC3I) and caspase-4/GSDMD pathway were determined by western blot. Autophagic vacuoles in cells were monitored with RFP-GFP-LC3 using fluorescence microscope.Results I/R caused caspase-4 activity and gasdermin D expression increase in vivo and in vitro. Overexpression of beclin-1 in heart tissue and CMECs suppressed the caspase-4 activity and decreased the levels of gasdermin D; meanwhile beclin1 overexpression also reduced IL-1β levels, promoted autophagy (P62 expression was inhibited while LC3II expression was increased) in the heart and CMECs. Interestingly, beclin1 overexpression increased animal survival and attenuated myocardial infarct size post-myocardial ischemia reperfusion.Conclusions Induction of beclin-1 signaling can be a potential therapeutic target in myocardial reperfusion-induced microvascular injury.
Correlation of antero-dorsal active contact location with weight gain after subthalamic nucleus deep brain stimulation: a case series
Authors: Eguchi Katsuki , Shirai Shinichi , Matsushima Masaaki , Kano Takahiro , Yamazaki Kazuyoshi , Hamauchi Shuji , Sasamori Toru , Hirata Kenji , KItagawa Mayumi , Otsuki Mika , Shiga Tohru , Houkin Kiyohiro , Sasaki Hidenao , Yabe Ichiro 
Published: April 2021

Abstract
Background Weight gain is a frequently reported side effect of subthalamic deep brain stimulation; however, the underlying mechanisms remain unclear. The active contact locations influence the clinical outcomes of subthalamic deep brain stimulation, but it is unclear whether weight gain is directly associated with the active contact locations. We aimed to determine whether weight gain is associated with the subthalamic deep brain stimulation active contact locations.Methods We enrolled 14 patients with Parkinson’s disease who underwent bilateral subthalamic deep brain stimulation between 2013 and 2019. Bodyweight and body mass index were measured before and one year following surgery. The Lead-DBS Matlab toolbox was used to determine the active contact locations based on magnetic resonance imaging and computed tomography. Fluorodeoxyglucose-positron emission tomography data were also acquired before and one year following surgery, and statistical parametric mapping was used to evaluate changes in brain metabolism. The relationship between weight and active contact locations was evaluated with a Spearman rank test with a corrected p-value < 0.008. We examined which brain regions’ metabolism fluctuation significantly correlated with increased BMI scores and PET data.Results The body mass index increase was 2.03 kg/m2 1 year post-surgery. Weight gain was correlated with anterior and dorsal locations of the left-side active contacts, as well as with lateral locations of the right-side active contacts. Furthermore, weight gain was correlated with increased metabolism in the left-side limbic and associative regions, including the middle temporal gyrus, inferior frontal gyrus, and orbital gyrus.Conclusions Although the mechanisms underlying weight gain following subthalamic deep brain stimulation are possibly multifactorial, our findings suggest that anterior subthalamic deep brain stimulation alters the activities in the limbic and associative cortical regions, which may then lead to weight gain. Weight gain could be prevented by avoiding stimulation to the anterior part of the subthalamic nucleus.