bluuebunny/crossref_metadata_2025_split huggingface dataset:
Serine-ubiquitination regulates Golgi morphology and the secretory pathway upon Legionella infection
Authors: Liu Yaobin , Mukherjee Rukmini , Bonn Florian , Colby Thomas , Matic Ivan , Glogger Marius , Heilemann Mike , Dikic Ivan 
Published: July 2021

AbstractSidE family of Legionella effectors catalyze non-canonical phosphoribosyl-linked ubiquitination (PR-ubiquitination) of host proteins during bacterial infection. SdeA localizes predominantly to ER and partially to the Golgi apparatus, and mediates serine ubiquitination of multiple ER and Golgi proteins. Here we show that SdeA causes disruption of Golgi integrity due to its ubiquitin ligase activity. The Golgi linking proteins GRASP55 and GRASP65 are PR-ubiquitinated on multiple serine residues, thus preventing their ability to cluster and form oligomeric structures. In addition, we found that the functional consequence of Golgi disruption is not linked to the recruitment of Golgi membranes to the growing Legionella-containing vacuoles. Instead, it affects the host secretory pathway. Taken together, our study sheds light on the Golgi manipulation strategy by which Legionella hijacks the secretory pathway and promotes bacterial infection.
Laparoscopic intraarterial catheterization with selective ICG fluorescence imaging in colorectal surgery
Authors: Heiliger Christian , Piecuch Jerzy , Frank Alexander , Andrade Dorian , von Ehrlich-Treuenstätt Viktor , Evtimova Dobromira , Kühn Florian , Werner Jens , Karcz Konrad 
Published: July 2021

AbstractThe quality of mesorectal resection is crucial for resection in rectal cancer, which should be performed by laparoscopy for better outcome. The use of indocyanine green (ICG) fluorescence is now routinely used in some centers to evaluate bowel perfusion. Previous studies have demonstrated in animal models that selective intra-arterial ICG staining can be used to define and visualize resection margins in rectal cancer. In this animal study, we investigate if laparoscopic intra-arterial catheterization is feasible and the staining of resection margins when performing total mesorectal excision with a laparoscopic medial to lateral approach is possible. In 4 pigs, laparoscopic catheterization of the inferior mesenteric artery (IMA) is performed using a seldinger technique. After a bolus injection of 10 ml ICG with a concentration of 0.25 mg/ml, a continuous intra-arterial perfusion was established at a rate of 2 ml/min. The quality of the staining was evaluated qualitatively. Laparoscopic catheterization was possible in all cases, and the average time for this was 30.25 ± 3.54 min. We observed a significant fluorescent signal in all areas of the IMA supplied, but not in other parts of the abdominal cavity or organs. In addition, the mesorectum showed a sharp border between stained and unstained tissue. Intraoperative isolated fluorescence augmentation of the rectum, including the mesorectum by laparoscopic catheterization, is feasible. Inferior mesenteric artery catheterization and ICG perfusion can provide a fluorescence-guided roadmap to identify the correct plane in total mesorectal excision, which should be investigated in further studies.
From the first touch to biofilm establishment by the human pathogen Candida glabrata: a genome-wide to nanoscale view
Authors: Cavalheiro Mafalda , Pereira Diana , Formosa-Dague Cécile , Leitão Carolina , Pais Pedro , Ndlovu Easter , Viana Romeu , Pimenta Andreia I. , Santos Rui , Takahashi-Nakaguchi Azusa , Okamoto Michiyo , Ola Mihaela , Chibana Hiroji , Fialho Arsénio M. , Butler Geraldine , Dague Etienne , Teixeira Miguel C. 
Published: July 2021

AbstractCandida glabrata is an opportunistic pathogen that adheres to human epithelial mucosa and forms biofilm to cause persistent infections. In this work, Single-cell Force Spectroscopy (SCFS) was used to glimpse at the adhesive properties of C. glabrata as it interacts with clinically relevant surfaces, the first step towards biofilm formation. Following a genetic screening, RNA-sequencing revealed that half of the entire transcriptome of C. glabrata is remodeled upon biofilm formation, around 40% of which under the control of the transcription factors CgEfg1 and CgTec1. Using SCFS, it was possible to observe that CgEfg1, but not CgTec1, is necessary for the initial interaction of C. glabrata cells with both abiotic surfaces and epithelial cells, while both transcription factors orchestrate biofilm maturation. Overall, this study characterizes the network of transcription factors controlling massive transcriptional remodelling occurring from the initial cell-surface interaction to mature biofilm formation.
Open-air printing of Cu2O thin films with high hole mobility for semitransparent solar harvesters
Authors: Sekkat Abderrahime , Nguyen Viet Huong , Masse de La Huerta César Arturo , Rapenne Laetitia , Bellet Daniel , Kaminski-Cachopo Anne , Chichignoud Guy , Muñoz-Rojas David 
Published: July 2021

AbstractCu2O is a promising p-type semiconductor for low-cost photovoltaics and transparent optoelectronics. However, low-cost and low-temperature fabrication of Cu2O films with good transport properties remains challenging, thus limiting their widespread adoption in devices. Here, we report Cu2O thin films of 20–80 nm thickness with hole mobility up to 92 cm2V−1s−1 using atmospheric-pressure spatial atomic layer deposition at temperatures below 260 °C, from a copper (I) hexafluoro-2,4-pentanedionate cyclooctadiene precursor. Raman spectroscopy indicates the presence of copper split vacancies and shows that the high hole mobility can be correlated to a low concentration of shallow acceptor defects. The optical bandgap of deposited films can be tuned between 2.08 eV and 2.5 eV, depending on the deposition temperature. All-oxide semitransparent Cu2O/ZnO solar harvesters are fabricated, showing efficiency values comparable to devices that incorporate much thicker Cu2O layers. Our work provides a promising approach towards cost-efficient, all-oxide solar harvesters, and for other (opto)electronic devices.
The anti-tumor effects of the combination of microwave hyperthermia and lobaplatin against breast cancer cells in vitro and in vivo
Authors: Li Xiaohu , Zhang Xin , Khan Inam Ullah , Guo Nina N. , Wang Bing , Guo Yifeng , Xiao Bufan , Zhang Yueshan , Chu Yimin , Chen Junsong , Guo Fang 
Published: July 2021

Abstract
Background: Breast cancer is the main lethal disease among females. The combination of lobaplatin and microwave hyperthermia plays a crucial role in several kinds of cancer in the clinic, but its possible mechanism in breast cancer has remained indistinct.
Methods: Mouse models were used to detect breast cancer progression. Cell growth was explored with MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulphonyl)-2H-tetrazolium) and colony formation assays. Cell migration and invasion were investigated with a transwell assay. Cell apoptosis was probed with flow cytometry. The expression of apoptosis-associated proteins was examined with Western blots.
Result: Combination treatment decreased breast cancer cell viability, colony formation, cell invasion and metastasis. In addition, the treatment-induced breast cancer cell apoptosis and autophagy, activated the c-Jun N-terminal kinase (JNK) signaling pathway, suppressed the protein kinase B (AKT)/mammalian target of rapamycin (mTOR) signaling pathway, and down-regulated IAP and Bcl-2 family protein expression.
Conclusion: These results indicate that lobaplatin is an effective breast cancer anti-tumor agent. Microwave hyperthermia was a useful adjunctive treatment. Combination treatment was more efficient than any single therapy. The possible mechanism for this effect was mainly associated with activation of the JNK signaling pathway, inactivation of the AKT/mTOR signaling pathway and down-regulation of the Bcl-2 and IAP families.
Full-length transcriptome assembly of andrias davidianus (amphibia: caudata) skin via hybrid sequencing
Authors: Bai Yu , Meng Yonglu , Luo Jianlin , Wang Hui , Li Guoyong , Li Can 
Published: July 2021

Abstract
The Chinese giant salamander, Andrias davidianus, is the largest amphibian species in the world; it is thus an economically and ecologically important species. The skin of A. davidianus exhibits complex adaptive structural and functional adaptations to facilitate survival in aquatic and terrestrial ecosystems. Here, we report the first full-length amphibian transcriptome from the dorsal skin of A. davidianus, which was assembled using hybrid sequencing and the PacBio and Illumina platforms. A total of 153,038 transcripts were hybrid assembled (mean length of 2039 bp and N50 of 2172 bp), and 133,794 were annotated in at least one database (nr, Swiss-Prot, KEGG, KOGs, GO, and nt). A total of 58,732, 68,742, and 115,876 transcripts were classified into 24 KOG categories, 1903 GO term categories, and 46 KEGG pathways (level 2), respectively. A total of 207,627 protein-coding regions, 785 transcription factors, 27,237 potential long non-coding RNAs, and 8299 simple sequence repeats were also identified. The hybrid-assembled transcriptome recovered more full-length transcripts, had a higher N50 contig length, and a higher annotation rate of unique genes compared with that assembled in previous studies using next-generation sequencing. The high-quality full-length reference gene set generated in this study will help elucidate the genetic characteristics of A. davidianus skin and aid the identification of functional skin proteins.
Finite control‐set model predictive direct speed control of a PMSM drive based on the Taylor series model
Authors: Wang Huimin , Zhang Senkai , Liu Wei , Geng Qiang , Zhou Zhanqing 
Published: July 2021

AbstractIn the traditional finite control‐set model predictive direct speed control (FCS‐MPDSC), the coupling items in the current equation have been ignored because of predictive complexity, leading to imperfect prediction accuracy and control performance. Meanwhile, the current error and speed error are both included in the weighted sum cost function. The two types of errors lead to the difficulty of weight coefficients' distribution, which can only rely on subjective experiences. Thus, an FCS‐MPDSC strategy based on the 2nd‐Taylor‐series model with quadratic cost function is proposed. The dynamic performance has been greatly improved because the input variables of the Taylor series model are unified as the basic voltage vector with the same control period. And the static error is also reduced as the weight coefficient matrix of the quadratic cost function is solved offline. On this basis, the verification experiments are carried out. The results show that a better dynamic and static performance is achieved at the same time by the proposed strategy.
Statistical multivariate monitoring of rolling bearings working under varying operational conditions
Authors: Wang Baoxiang , Pan Hongxia 
Published: July 2021

AbstractIn recent years, statistical data‐driven approaches have been developed for health monitoring of rolling bearings working under variable operational conditions. Due to the reliance on reference models, most of them encounter with the model mismatch problem. A novel statistical multivariate health monitoring framework is proposed in this article to determine the initial degradation of rolling bearings. On the one hand, multivariate statistical parameters are extracted from original vibration signals to indicate bearing's degradation trajectories from different perspectives, a dynamic autoencoder neural network is extended with a matrix augmentation trick for discovering both dynamic and nonlinear latent representation in the high‐dimensional features. On the other hand, an eccentricity index is introduced for measuring information variations in the latent representation, such that bearing health development can be revealed timely. Then, an adaptive threshold is exploited for online monitoring of bearing damage. The effectiveness of the proposed work is substantiated using real vibration signals acquired from two different bearing degradation test rigs. The monitoring performance of the proposed work is evaluated in terms of false alarm rate (FAR). Experimental results confirm that the proposed work can identify early bearing damage under variable working conditions, by circumventing the model mismatch problem. It is found to be more superior than existing techniques.
The importance of seismic protection of strategic objectives, in the area of influence of useful rock quarries, in which blasting works are carried out
Authors: Garaliu-Buşoi Bogdan , Kovacs Attila , Gheorghiosu Edward , Rădeanu Cristian , Miclea Olga 
Published: July 2021

In this scientific article was studied the main methods for assessing the seismic effect generated during the blasting operations with explosives, from quarries on essential infrastructure elements, in the analyzed situation - railway tunnel and supporting pillars of the railway superstructure in the adjacent area of the quarry, regarding the most appropriate technique for estimating and assessing the seismic effect through the specificity of the evaluated parameter.
In order to be able to carry out an activity with as little impact as possible on the environment, the economic operator carrying out career blasting works must apply technical measures to ensure the protection of civil / industrial objectives in the area close to carrying out these works.
The establishment of measures leading to the maintenance of the stability and integrity of the objectives, as well as to the protection of the environment, is based on an artificial earthquake assessment process, which highlights the possible effects on the adjacent area.
The evaluation of the seismic effect can be done by monitoring some parameters that characterize the seismic waves produced by quarry blasting such as: frequency of oscillations, amplitude of movement, soil particles oscillations velocity, acceleration of oscillations, duration of their manifestation.
Comparative measurements between the results achieved with reference method and the optical method for determination of PM10 dusts in ambient air
Authors: Kovacs Marius , Călămar Angelica-Nicoleta , Toth Lorand , Simion Sorin , Simion Alexandru 
Published: July 2021

The current paper aims to determine the actuality of the optical method of measuring PM10 dusts powders compared to the reference method (gravimetric method). According to the Integrated National Plan in the field of Energy and Climate Change 2021-2030, Romania must implement a program to reduce impact on atmosphere, including air quality monitoring, thus leading to an increase in the number of monitoring points and an increase in the number of samples required for monitoring.
Currently, PM10 dust monitoring stations use the gravimetric method, consisting in absorbing a known volume of air on filters and weighing the dust deposited on them. As the use of this method requires a minimum of 3 days, it is necessary to apply an alternative measuring method, with short response time, namely the optical method.
Parallel experimentation of the two measuring devices that use different principles was performed in several series of measurements at an economic agent that has the obligation to monitor dust at premises borders. Results obtained from comparison of the two devices can support future researchers in order to find limitations of the optical method of measuring PM10 dusts and to validate the method, in order to use it routinely.
Pharmacognostic Evaluation of Ten Species of Medicinal Importance of Cecropia: Current Knowledge and Therapeutic Perspectives
Authors: Rivera-Mondragón Andrés , Ortíz Orlando O. , Gupta Mahabir P. , Caballero-George Catherina 
Published: July 2021

AbstractThis work covers a systematic review of literature about the genus Cecropia from 1978 to 2020, emphasizing the analysis of 10 of the most relevant species and their associated biological activities. Cecropia is a neotropical genus, which comprises about 61 native species in the American continent where it is known to be part of the traditional medicine of numerous countries. Secondary metabolites described for this genus showed an elevated structural and functional diversity, where polyphenols have been the most abundant. Based on this diversity, Cecropia phytochemicals represent an important source of potential therapeutic agents yet to be exploited. This review also highlights the effectiveness of combining chemometrics and ultra-performance liquid chromatography-tandem mass spectrometry as a novel approach to successfully single out Cecropia species phytochemicals. While the medicinal use of Cecropia species is officially recognized in National Pharmacopoeias and Formularies of several Latin American countries, it is important to recognize that these phytomedicines are complex mixtures requiring a thorough understanding of their chemical composition and their correlation with biological activities to guarantee their quality, safety, and efficacy.
Prevalence of Primary Aldosteronism Across the Stages of Hypertension
Based on a New Combined Overnight Test
Authors: Voulgaris Nick , Tyfoxylou Ernestini , Vlachou Sophia , Kyriazi Evagelia , Gravvanis Chris , Kapsali Chara , Markou Athina , Papanastasiou Labrini , Gryparis Alexandros , Kassi Eva , Chrousos George , Kaltsas Gregory , Piaditis George 
Published: July 2021

AbstractPrimary aldosteronism (PA) is the most common endocrine cause of arterial
hypertension. Despite the increasing incidence of hypertension worldwide, the
true prevalence of PA in hypertension was only recently recognized. The
objective of the work was to estimate the prevalence of PA in patients at
different stages of hypertension based on a newly developed screening-diagnostic
overnight test. This is a prospective study with hypertensive patients
(n=265) at stage I (n=100), II (n=88), and III
(n=77) of hypertension. A group of 103 patients with essential
hypertension without PA was used as controls. PA diagnosis was based on a
combined screening-diagnostic overnight test, the
Dexamethasone-Captopril-Valsartan Test (DCVT) that evaluates aldosterone
secretion after pharmaceutical blockade of angiotensin-II and
adrenocorticotropic hormone. DCVT was performed in all participants
independently of the basal aldosterone to renin ratio (ARR). The calculated
upper normal limits for post-DCVT aldosterone levels [3 ng/dl (85
pmol/l)] and post-DCVT ARR [0.32
ng/dl/μU/ml (9 pmol/IU)] from controls,
were applied together to establish PA diagnosis. Using these criteria PA was
confirmed in 80 of 265 (30%) hypertensives. The prevalence of PA was:
21% (21/100) in stage I, 33% (29/88) in stage
II, and 39% (30/77) in stage III. Serum K+
levels were negatively correlated and urinary K+ was
positively correlated in PA patients with post-DCVT ARR
(r=–0.349, p <0.01, and r=0.27, p <0.05
respectively). In conclusion, DCVT revealed that PA is a highly prevalent cause
of hypertension. DCVT could be employed as a diagnostic tool in all subjects
with arterial hypertension of unknown cause.
Age Moderates the Impact of TRAbs on Thyroid Hormones and Hepatic
Function in Patients with Graves’ Disease
Authors: Guo Kai , Ling Hongwei , Zhou Xiaoyan , Ying Changjiang 
Published: July 2021

AbstractThyrotropin receptor antibodies (TRAbs) play a significant role in the course of
hepatic dysfunction (HDF) in patients with Graves’ disease (GD).
However, few studies have considered the factors that influence the
relationships among TRAbs, thyroid hormone levels, and hepatic function in
subjects with newly diagnosed GD. Here we investigated the associations of TRAbs
with thyroid hormones and hepatic function and assessed potential factors that
can influence these associations among patients with GD. A total of 368 patients
newly diagnosed with GD were collected in this cross-sectional study. Patients
who had received antithyroid drugs, radioactive iodine, or surgery were
excluded. Levels of TRAbs and thyroid hormones and hepatic function were
recorded. Linear and binary logistic regression analysis models were applied to
investigate associations among these variables after adjusting for confounding
characteristics. There was a significant difference in TRAbs indices between the
HDF and normal hepatic function groups (p <0.05). After adjusting for
confounders, the relationship between TRAbs and thyroid hormones was nonlinear,
showing a curve with an initial positive slope and a subsequent flattening (p
<0.05). Higher TRAbs were associated with HDF [odds ratio (OR) 1.036,
95% confidence interval (CI) 1.018–1.053 per 1-IU/l
increase]. These associations were modified by age, but not by gender, smoking
status, Graves’ orbitopathy, thyroid-peroxidase antibody levels, or
thyroglobulin antibody levels. In younger patients, increasing TRAbs were
correlated with higher thyroid hormones and HDF (OR 1.034, 95% CI
1.017–1.052) per1-IU/l increase). In older patients, TRAbs were
not correlated with thyroid hormones or HDF (OR 1.024, 95% CI
0.993–1.056) per 1-IU/l increase. Age can affect the impact of
TRAbs on thyroid hormone levels and hepatic function in GD. TRAb measurement can
have good predictive value in younger patients.
Treatment Strategies for Dopamine Agonist-Resistant and Aggressive Prolactinomas: A Comprehensive Analysis of the Literature
Authors: Sari Ramazan , Altinoz Meric A. , Ozlu Eylem Burcu Kahraman , Sav Aydin , Danyeli Ayca Ersen , Baskan Ozdil , Er Ozlem , Elmaci Ilhan 
Published: July 2021

AbstractDespite most of the prolactinomas can be treated with endocrine therapy and/or surgery, a significant percentage of these tumors can be resistant to endocrine treatments and/or recur with prominent invasion into the surrounding anatomical structures. Hence, clinical, pathological, and molecular definitions of aggressive prolactinomas are important to guide for classical and novel treatment modalities. In this review, we aimed to define molecular endocrinological features of dopamine agonist-resistant and aggressive prolactinomas for designing future multimodality treatments. Besides surgery, temozolomide chemotherapy and radiotherapy, peptide receptor radionuclide therapy, estrogen pathway modulators, progesterone antagonists or agonists, mTOR/akt inhibitors, pasireotide, gefitinib/lapatinib, everolimus, and metformin are tested in preclinical models, anecdotal cases, and in small case series. Moreover, chorionic gonadotropin, gonadotropin releasing hormone, TGFβ and PRDM2 may seem like possible future targets for managing aggressive prolactinomas. Lastly, we discussed our management of a unique prolactinoma case by asking which tumors’ proliferative index (Ki67) increased from 5–6% to 26% in two subsequent surgeries performed in a 2-year period, exerted massive invasive growth, and secreted huge levels of prolactin leading up to levels of 1 605 671 ng/dl in blood.