bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cold spell variability in Europe in relation to the degree of climate continentalism in 1981–2018 period
Authors: Jarzyna Krzysztof , Krzyżewska Agnieszka 
Published: March 2021

AbstractSeasonal and multiannual variability of the number, duration and severity of cold spells in Europe were analysed in this paper in cold seasons starting with 1981/1982 through 2017/2018. Data were used from seven weather stations characterised by different degrees of climate continentalism. Cold spells did not occur in the oceanic climate of southeastern England. In the Netherlands only a few cold spells were identified in the entire study period lasting 31 or 67 days in total, depending on the method used. The number, duration and severity of cold spells increased toward the east. In the continental climate of the European part of Russia, 163 or 74 cold spells were identified. Their total duration exceeded 1300 days. In Western Europe the potential period when cold spells occurred lasted about 50 days, from the middle of December to the beginning of February. In Eastern Europe the period when cold spells occurred lasted about 140 days, from the beginning of November to the second half of March. The longest and the most severe cold spells were concentrated in four periods: 1984/1985 to 1986/1987, 1993/1994 to 1996/1997, 2001/2002 to 2005/2006 and 2009/2010 to 2011/2012. Statistically significant trends in the number, duration and severity of cold spells were not evident for the locations analysed over the period from 1981 to present.
Auriferous pyrite formed by episodic fluid inputs in the Akeshi and Kasuga high-sulfidation deposits, Southern Kyushu, Japan
Authors: Ishida Mizuki , Romero Rurik , Leisen Mathieu , Yasukawa Kazutaka , Nakamura Kentaro , Barra Fernando , Reich Martin , Kato Yasuhiro 
Published: March 2021

AbstractPyrite geochemistry has proven useful for tracking changes in the composition and physico-chemical conditions of hydrothermal fluids in ore-forming environments. Here, we investigated the microtextural features and chemical composition of pyrite, a main Au-bearing phase in the Akeshi and Kasuga deposits (Southern Kyushu, Japan), to better constrain the ore-forming processes in these high-sulfidation epithermal Au deposits. Despite the widespread distribution of Au-bearing pyrite in both deposits, no visible Au minerals coexist with pyrite. However, in situ laser ablation inductively coupled plasma mass spectrometry results show that Au concentrations in pyrite vary from below the detection limit to 41 ppm and are positively correlated with Cu (r = 0.4; up to 7400 ppm) and Bi concentrations (r = 0.44; up to 640 ppm). In both deposits, high Cu and Au concentrations occur in small (< 25 μm) anhedral grains of pyrite, which are interpreted to have rapidly crystallized from the ore-forming hydrothermal fluid. In addition, dissolution–reprecipitation textures and thin, concentric, Cu-rich overgrowths were identified in a number of larger (> 25 μm) pyrite grains and aggregates. These abrupt changes in the trace element compositions of pyrite grains likely record episodic metal-rich fluid inputs. We also propose that gold adsorption onto growing pyrite surfaces played a key role in the mineralization of these deposits.
Public understanding of female genital anatomy and pelvic organ prolapse (POP); a questionnaire-based pilot study
Authors: El-Hamamsy Dina , Parmar Chanel , Shoop-Worrall Stephanie , Reid Fiona M. 
Published: March 2021

Abstract
Introduction and hypothesis
Health literacy underpins informed consent and shared decision-making. In gynaecology, this includes understanding of normal anatomy and urogenital disease. This study evaluated public knowledge of external female genital anatomy and pelvic organ prolapse (POP).

Methods
A questionnaire study asked participants for their demographics and to label a female external genitalia diagram and included free-text questions on POP, its symptoms and treatment. Questionnaires were distributed at general outpatient (OPD) and urogynaecology (UG) departments at a UK teaching hospital. Differences in the number of correct anatomy labels between participant genders were assessed via chi-squared tests and, within female participants, multivariable linear and logistic regressions assessed associations with increasing correct anatomical labels and an understanding (versus no understanding) of POP, respectively.

Results
Within 191 (n = 160 OPD, n = 31 UG), 9/103 (9%) labelled all anatomical structures correctly. Females had more correct labels (median 1, IQR 0,3) versus males (median 0, IQR 0,1), P = 0.022). Higher education (vs. < secondary) and white ethnicity were associated with greater numbers of correct labels [coefficient (95% CI): 1.05 (0.14, 1.96), P = 0.024, 1.45 (0.58, 2.33), P = 0.001 respectively]. Fifty-three per cent understood POP. POP understanding increased with increasing age, white ethnicity (OR: 4.38, 95% CI: 1.36, 14.08, P = 0.013) and more correct anatomy labels (OR: 1.43, 95% CI 1.14, 1.79, P = 0.002). Of those who understood POP, only 35% identified “bulge” as a symptom and 7% physiotherapy as a treatment option.

Conclusion
There was poor public understanding of external female genital anatomy and POP, which may have significant implications for health-seeking, shared decision-making and informed consent.
Selected tools to visualize membrane interactions
Authors: Grothe Tobias , Nowak Julia , Jahn Reinhard , Walla Peter Jomo 
Published: March 2021

AbstractIn the past decade, we developed various fluorescence-based methods for monitoring membrane fusion, membrane docking, distances between membranes, and membrane curvature. These tools were mainly developed using liposomes as model systems, which allows for the dissection of specific interactions mediated by, for example, fusion proteins. Here, we provide an overview of these methods, including two-photon fluorescence cross-correlation spectroscopy and intramembrane Förster energy transfer, with asymmetric labelling of inner and outer membrane leaflets and the calibrated use of transmembrane energy transfer to determine membrane distances below 10 nm. We discuss their application range and their limitations using examples from our work on protein-mediated vesicle docking and fusion.
Linking population size structure, heat stress and bleaching responses in a subtropical endemic coral
Authors: Lachs Liam , Sommer Brigitte , Cant James , Hodge Jessica M. , Malcolm Hamish A. , Pandolfi John M. , Beger Maria 
Published: March 2021

AbstractAnthropocene coral reefs are faced with increasingly severe marine heatwaves and mass coral bleaching mortality events. The ensuing demographic changes to coral assemblages can have long-term impacts on reef community organisation. Thus, understanding the dynamics of subtropical scleractinian coral populations is essential to predict their recovery or extinction post-disturbance. Here we present a 10-yr demographic assessment of a subtropical endemic coral, Pocillopora aliciae (Schmidt-Roach et al. in Zootaxa 3626:576–582, 2013) from the Solitary Islands Marine Park, eastern Australia, paired with long-term temperature records. These coral populations are regularly affected by storms, undergo seasonal thermal variability, and are increasingly impacted by severe marine heatwaves. We examined the demographic processes governing the persistence of these populations using inference from size-frequency distributions based on log-transformed planar area measurements of 7196 coral colonies. Specifically, the size-frequency distribution mean, coefficient of variation, skewness, kurtosis, and coral density were applied to describe population dynamics. Generalised Linear Mixed Effects Models were used to determine temporal trends and test demographic responses to heat stress. Temporal variation in size-frequency distributions revealed various population processes, from recruitment pulses and cohort growth, to bleaching impacts and temperature dependencies. Sporadic recruitment pulses likely support population persistence, illustrated in 2010 by strong positively skewed size-frequency distributions and the highest density of juvenile corals measured during the study. Increasing mean colony size over the following 6 yr indicates further cohort growth of these recruits. Severe heat stress in 2016 resulted in mass bleaching mortality and a 51% decline in coral density. Moderate heat stress in the following years was associated with suppressed P. aliciae recruitment and a lack of early recovery, marked by an exponential decrease of juvenile density (i.e. recruitment) with increasing heat stress. Here, population reliance on sporadic recruitment and susceptibility to heat stress underpin the vulnerability of subtropical coral assemblages to climate change.
Experimental settling, floatation and compaction of plagioclase in basaltic melt and a revision of melt density
Authors: Krättli Giuliano , Schmidt Max W. 
Published: March 2021

AbstractCentrifuge-assisted piston cylinder experiments were conducted on plagioclase in basaltic melt at 1140–1250 °C, 0.42–0.84 GPa and mostly 1000 g. One set of experiments assesses the settling velocity of a dilute plagioclase suspension; a second sinks or floats plagioclase in a MORB-type melt exploring conditions of neutral buoyancy; and a third set examines floatation of plagioclase from an evolved lunar magma ocean composition. A compaction rate for plagioclase cumulates is established. The experiments demonstrate that neutral density of plagioclase An74 in a MOR-type tholeiitic basalt occurs at 0.59 ± 0.04 GPa (1200 °C), contrasting predictions by present models on melt density which yield a density inversion pressure at 0.10–0.15 GPa. In nature, the level of neutral buoyancy depends on melt composition; nevertheless, for the onset of plagioclase crystallization in dry tholeiitic basalts, our result is robust. As the molar volume of plagioclase is well known, the experimentally determined pressure of neutral buoyancy indicates a correction of -1.6% to previous density models for silicate melts. It follows that for (tholeiitic) layered mafic intrusions, plagioclase is negatively buoyant for early, relatively primitive, parent melts. In contrast, the extreme Fe enrichment of a fractionating lunar magma ocean leads to melt densities that let anorthite always float. Compaction φ/φ0 of experimental plagioclase cumulates is quantified to φ/φ0 = − 0.0582 log (Δρ·h·a·t) + 1.284, where φ0 is the porosity after settling (67 ± 2%), h the cumulate pile height, a acceleration and φ porosity as a function of time t. Gravitational-driven compaction in tens of m-thick plagioclase cumulate in basaltic magmas reaches down to ~ 40% porosity within hundreds of years, a timescales competing with characteristic cooling times of cumulate layers of mafic intrusions. To achieve plagioclase modes > 80% due to compaction, an additional overload of ~ 100 m (layers) of mafic minerals would be required. Compaction of a lunar anorthosite crust of 35 km to 20% porosity (i.e. ~ 90% plagioclase after crystallization of the interstitial melt) would require 30 kyrs.
Transvaginal Hybrid-NOTES procedures—do they have a negative impact on pregnancy and delivery?
Authors: Thomaidis Panagiotis , Weltermann Niklas J. , Seefeldt Claudia S. , Richards Dana C. , Sauerwald Axel , Heiss Markus M. , Bulian Dirk R. 
Published: March 2021

Abstract
Purpose
We conducted a retrospective observational study in order to identify negative effects of NOTES procedures (Natural Orifice Transluminal Endoscopic Surgery) with transvaginal specimen removal on pregnancy and delivery.

Methods
From the total population of 299 patients in our NOTES registry, we tried to contact the 121 patients who were of reproductive age (≤ 45 years) at the time of a transvaginal NOTES procedure. They were interviewed by telephone regarding their desire for children, post NOTES-operation pregnancies, and type of delivery using a structured questionnaire. The collected data was analyzed and compared with current data.

Results
We were able to contact 76 patients (follow-up rate: 62.8%) with a median follow-up of 77 months after surgery (33–129 months). Twenty of 74 participating patients had a desire for children (27.0%). One of them and another's male partner were diagnosed as infertile. Regarding the remaining 18 patients, 14 became pregnant, and three of them became pregnant twice. Considering these 17 pregnancies, there was one miscarriage (5.9%) and one twin birth (5.9%). On average, childbirth occurred 44 months after the NOTES procedure. With regard to the type of delivery, 10 vaginal births (58.8%) and 7 caesarean sections (41.2%) occurred. Thus, the rate of fulfilled desire for children was 77.8%. Compared with the literature, no difference to the normal course could be detected.

Conclusion
There is no sign that the transvaginal approach in Hybrid-NOTES, with removal of the specimen through the vagina, has a negative effect on conception, the course during pregnancy, or the type of delivery.
Didelphis spp. opossums and their parasites in the Americas: A One Health perspective
Authors: Bezerra-Santos Marcos Antônio , Ramos Rafael Antonio Nascimento , Campos Artur Kanadani , Dantas-Torres Filipe , Otranto Domenico 
Published: March 2021

AbstractMedium sized opossums (Didelphisspp.) are among the most fascinating mammals of the Americas, playing important ecological roles (e.g., dispersal of seeds and control of insect populations) in the environment they inhabit. Nevertheless, as synanthropic animals, they are well adapted to human dwellings, occupying shelters within the cities, peripheral areas, and rural settings. These marsupials can harbor numerous pathogens, which may affect people, pets, and livestock. Among those, some protozoa (e.g.,Leishmania infantum,Trypanosoma cruzi,Toxoplasma gondii), helminths (e.g.,Ancylostoma caninum,Trichinella spiralis,Alaria marcianae,Paragonimusspp.) and arthropods (e.g., ticks, fleas) present substantial public health and veterinary importance, due to their capacity to cause disease in humans, domestic animals, and wildlife. Here, we reviewed the role played by opossums on the spreading of zoonotic parasites, vectors, and vector-borne pathogens, highlighting the risks of pathogens transmission due to the direct and indirect interaction of humans and domestic animals withDidelphisspp. in the Americas.
Mineralogical and Micro-structural Investigation into the Mechanical Behaviour of a Soft Calcareous Mudstone
Authors: Simpson D. , Rouainia M. , Elia G. 
Published: March 2021

AbstractThe construction industry in Abu Dhabi is thriving and its coastline has some of the most ambitious structures in the world. Whilst the subsurface evaporitic and calcareous soft rocks of this region are of great geological interest, they are relatively poorly understood from a geotechnical engineering perspective, forcing foundation designs to be overly conservative. Understanding the stiffness of the underlying geology at small strains is of great importance for the accurate estimation of ground movements around excavations and foundations, and yet routine post-SI laboratory testing programmes tend to focus on basic rock mechanics tests such as UCS tests. These procedures are generally unsuitable for use with calcareous rocks due to their friable and moisture sensitive nature, and rarely obtain parameters representative of actual in situ behaviour. The calcareous mudstone investigated in this paper has mechanical and structural characteristics falling between those of a soil and those typical of a rock and, as such, requires a geotechnical testing approach that combines methods from both soil and rock mechanics disciplines. The mineralogical, micro-structural and mechanical characteristics of this lithology have been examined via a suite of testing techniques, including XRPD, SEM, advanced triaxial with bender elements, along with industry standard procedures. Shearing, tensile and consolidation behaviours have been explored. Examination of the micro- and macro-scale features of this material shows it to be highly structured, with strength and stiffness being controlled by inter-granular bonding of Dolomite grains, as well as by mean effective stress state and rate of strain. The presence of fibrous Palygorskite acts to reduce the degree of bonding, causing specimens rich in this clay mineral to have a more ductile mechanical behaviour.
Reducing the dosing frequency of selective digestive tract decontamination to three times daily provides effective decontamination of Gram-negative bacteria
Authors: de la Court Jara R. , Sigaloff Kim C. E. , Groot Thomas , van der Spoel Johan I. , Schade Rogier P. 
Published: March 2021

AbstractThis study evaluated the effectiveness of selective digestive tract decontamination (SDD) application three times daily (t.i.d.) compared to the standard four times daily (q.i.d.). Retrospective equivalence (combined non-inferiority and non-superiority design) study with a before-and-after design on a tertiary ICU in which the SDD frequency was reduced from q.i.d. to t.i.d. All patients with ICU admissions ≥72h and with ≥2 surveillance cultures collected on different dates were included in this study. We compared successful decontamination of Gram-negative bacteria (GNB). Furthermore, time to decontamination, ICU-acquired GNB bacteraemia and 28-day mortality were compared between the two groups. In total 1958 ICU admissions (1236 q.i.d., 722 t.i.d). Decontamination was achieved during the first week of admission in 77% and 76% of patients receiving SDD q.i.d and t.i.d., respectively. Successful decontamination within 14 days (without consecutive acquisition of Gram-negative bacteria) was achieved in 69.3% of the admissions with q.i.d. versus 66.8% in t.i.d. SDD (p-value = 0.2519). The proportions of successful decontamination of GNB were equivalent in both groups (−0.025, 98% CI: −0.087; 0.037). There was no significant difference in time to decontamination between the two regimens (log-rank test p-value = 0.55). Incidence (episodes/1000 days) of ICU-acquired GNB bacteraemia was 0.9 in both groups, and OR for death at day 28 in the t.i.d. group compared to the q.i.d. group was 0.99 (95% confidence interval, 0.80–1.21). This study shows that a t.i.d. application regimen achieves similar outcomes to the standard q.i.d. regime, for both microbiological and clinical outcome measures.
X-ray Ptychography Imaging of Human Chromosomes After Low-dose Irradiation
Authors: Bhartiya Archana , Batey Darren , Cipiccia Silvia , Shi Xiaowen , Rau Christoph , Botchway Stanley , Yusuf Mohammed , Robinson Ian K. 
Published: March 2021

AbstractStudies of the structural and functional role of chromosomes in cytogenetics have spanned more than 10 decades. In this work, we take advantage of the coherent X-rays available at the latest synchrotron sources to extract the individual masses of all 46 chromosomes of metaphase human B and T cells using hard X-ray ptychography. We have produced ‘X-ray karyotypes’ of both heavy metal–stained and unstained spreads to determine the gain or loss of genetic material upon low-level X-ray irradiation doses due to radiation damage. The experiments were performed at the I-13 beamline, Diamond Light Source, Didcot, UK, using the phase-sensitive X-ray ptychography method.
Mechanisms of thermal treatment on two dominant copepod species in O3/BAC processing of drinking water
Authors: Jiang Wei , Dong Sheng , Xu Fangfang , Chen Jing , Gong Chen , Wang Antai , Hu Zhangli 
Published: March 2021

AbstractPhyllodiaptomus tunguidus and Heliodiaptomus falxus are dominant copepods species in drinking water processing plants in southern China. With a potential penetration risk, the breeding and leakage of copepods are drawing more and more attention in recent years. The current study provided a thermal treatment method to control copepods and their eggs. Results showed that: (1) the immediate death rates of P. tunguidus and H. falxus after heated to 34–40 °C for 5 min are positively correlated to the treatment temperatures (P < 0.01), and all individuals of the both species were eliminated after heated at 40 °C for 5 min; (2) overall hatching rates of P. tunguidus eggs were negatively correlated with treatment temperatures (P < 0.01) between 39–45 °C, with zero percent hatched after treatment at 45 °C for 5 min; (3) hatching rates of H. falxus were negatively correlated with treatment temperatures (P < 0.01) between 37–41 °C, with no nauplii hatched when treated at 41 °C for 5 min; (4) paraffin section histological examination indicated that thermal treatment caused severe damage to internal organs and egg structure. Finally, based on the experimental data, the application of the thermal treatment method was discussed in ozonation combined with biological activated carbon (O3/BAC) processing of drink water treatment.
Singularities, Black Holes, and Cosmic Censorship: A Tribute to Roger Penrose
Authors: Landsman Klaas 
Published: March 2021

AbstractIn the light of his recent (and fully deserved) Nobel Prize, this pedagogical paper draws attention to a fundamental tension that drove Penrose’s work on general relativity. His 1965 singularity theorem (for which he got the prize) does not in fact imply the existence of black holes (even if its assumptions are met). Similarly, his versatile definition of a singular space–time does not match the generally accepted definition of a black hole (derived from his concept of null infinity). To overcome this, Penrose launched his cosmic censorship conjecture(s), whose evolution we discuss. In particular, we review both his own (mature) formulation and its later, inequivalent reformulation in thepdeliterature. As a compromise, one might say that in “generic” or “physically reasonable” space–times, weak cosmic censorship postulates theappearance and stability of event horizons, whereas strong cosmic censorship asks for theinstability and ensuing disappearance of Cauchy horizons. As anencore, an “Appendix” by Erik Curiel reviews the early history of thedefinitionof a black hole.