bluuebunny/crossref_metadata_2025_split huggingface dataset:
Empowering Maasai women behind the camera: Photovoice as a tool for trachoma control
Authors: Mtuy Tara B. , Mepukori Jeremiah , Lankoi Joseph , Lees Shelley 
Published: July 2021

Abstract
Background
Photovoice is a method used to help engage community members to understand local realities and promote social change. Photovoice uses cameras in the hands of participants as a tool to visually document a specified topic. Photos taken by participants allow for reflection and stimulate dialog on an issue to ideally lead to social change. Trachoma, hyperendemic in Maasai communities in Northern Tanzania, is the commonest infectious cause of blindness worldwide, caused by chlamydia trachomatis. The bacterial infection commonly occurs in childhood and over many years repeated infections leads to inflammation and scarring of the eyelid. Often as adults this leads to the upper eyelid turning inward and eyelashes scratching the eye, resulting in pain and eventually blindness. We used photovoice as a tool for Maasai women to share their lived experiences of educating peers on trachoma and ultimately empowering women in this society.

Methods
This public engagement intervention was conducted September thru October 2017. We held a workshop on trachoma control for 20 Maasai women including use of photovoice method. Women were asked to disseminate information from the trachoma control workshop to their community and to capture their experiences using disposable cameras. Five weeks post-workshop we facilitated a discussion and women displayed photos of the successes and challenges they encountered as advocates for trachoma control in their community.

Intervention Outcomes
It was observed throughout the process and at the photo discussion meeting, that women articulated empowerment by this experience; as educators, agents of change and a source of valued information.

Conclusion
Photovoice should be considered for future interventions as a communication tool on health issues and to empower women to be ambassadors for health promotion.
The use of a pedicled buccal fat pad for reconstruction of posterior mandibular defects
Authors: Lee Hyen Woo , Hong Sung ok , Bae Heeyeon , Shin Youngjin , Jee Yu-jin 
Published: July 2021

Abstract
Background
The pedicled buccal fat pad has been used for a long time to reconstruct oral defects due to its ease of flap formation and few complications. Many cases related to reconstruction of defects in the maxilla, such as closing the oroantral fistula, have been reported, but cases related to the reconstruction of defects in the mandible are limited. Under adequate anterior traction, pedicled buccal fat pad can be a reliable and effective method for reconstruction of surgical defects in the posterior mandible.

Case presentation
This study describes two cases of reconstruction of surgical oral defects in the posterior mandible, all of which were covered by a pedicled buccal fat pad. The size of the flap was sufficient to perfectly close the defect without any tension. Photographic and radiologic imaging showed successful closure of the defects and no problems were noted in the treated area.

Conclusion
In conclusion, the pedicled buccal fat pad graft is a convenient and reliable method for the reconstruction of surgical defects on the posterior mandible.
Characteristic mutations induced in the small intestine of Msh2-knockout gpt delta mice
Authors: Aoki Yasunobu , Ohno Mizuki , Matsumoto Michiyo , Matsumoto Michi , Masumura Kenichi , Nohmi Takehiko , Tsuzuki Teruhisa 
Published: July 2021

Abstract
Background
Base pair mismatches in genomic DNA can result in mutagenesis, and consequently in tumorigenesis. To investigate how mismatch repair deficiency increases mutagenicity under oxidative stress, we examined the type and frequency of mutations arising in the mucosa of the small intestine of mice carrying a reporter gene encoding guanine phosphoribosyltransferase (gpt) and in which the Msh2 gene, which encodes a component of the mismatch repair system, was either intact (Msh2+/+::gpt/0; Msh2-bearing) or homozygously knockout (KO) (Msh2−/−::gpt/0; Msh2-KO).

Results
Gpt mutant frequency in the small intestine of Msh2-KO mice was about 10 times that in Msh2-bearing mice. Mutant frequency in the Msh2-KO mice was not further enhanced by administration of potassium bromate, an oxidative stress inducer, in the drinking water at a dose of 1.5 g/L for 28 days. Mutation analysis showed that the characteristic mutation in the small intestine of the Msh2-KO mice was G-to-A transition, irrespective of whether potassium bromate was administered. Furthermore, administration of potassium bromate induced mutations at specific sites in gpt in the Msh2-KO mice: G-to-A transition was frequently induced at two known sites of spontaneous mutation (nucleotides 110 and 115, CpG sites) and at nucleotides 92 and 113 (3′-side of 5′-GpG-3′), and these sites were confirmed to be mutation hotspots in potassium bromate-administered Msh2-KO mice. Administration of potassium bromate also induced characteristic mutations, mainly single-base deletion and insertion of an adenine residue, in sequences of three to five adenine nucleotides (A-runs) in Msh2-KO mice, and elevated the overall proportion of single-base deletions plus insertions in Msh2-KO mice.

Conclusions
Our previous study revealed that administration of potassium bromate enhanced tumorigenesis in the small intestine of Msh2-KO mice and induced G-to-A transition in the Ctnnb1 gene. Based on our present and previous observations, we propose that oxidative stress under conditions of mismatch repair deficiency accelerates the induction of single-adenine deletions at specific sites in oncogenes, which enhances tumorigenesis in a synergistic manner with G-to-A transition in other oncogenes (e.g., Ctnnb1).
The analysis of sacrum and coccyx length measured with computerized tomography images depending on sex
Authors: Bakici Rukiye Sumeyye , Oner Zulal , Oner Serkan 
Published: July 2021

Abstract
Background
Sex estimation is vital in establishing an accurate biological profile from the human skeleton, as sex influences the analysis of other elements in both Physical and Forensic Anthropology and Legal Medicine. The present study was conducted to analyze the sex differences between the sacrum and coccyx length based on the measurements calculated with computed tomography (CT) images. One hundred case images (50 females, 50 males) who were between the ages of 25 and 50 and admitted by the emergency department between September 2018 and June 2019 and underwent CT were included in the study. Eighteen lengths, 4 curvature lengths, and 2 regions were measured in sagittal, coronal and transverse planes with orthogonal adjustment for three times.

Results
It was stated that the mean anterior and posterior sacral length, anterior and posterior sacrococcygeal length, anterior and posterior sacral curvature length, anterior coccygeal curvature length, sacral area, lengths of transverse lines 1, 2, 3 and 4, sacral first vertebra transverse and sagittal length measurements were longer in males when compared to females (p < 0.05). It was noted that the parameter with the highest discrimination value in the receiver operating characteristic (ROC) analysis was the sacral area (AUC = 0.88/Acc = 0.82). Based on Fisher’s linear discriminant analysis findings, the discrimination rate was 96% for males, 92% for females and the overall discrimination rate was 94%.

Conclusions
It was concluded that the fourteen parameters that were indicated as significant in the present study could be used in anthropology, Forensic Medicine and Anatomy to predict sex.
Modeling the dynamics of Lassa fever in Nigeria
Authors: Ojo Mayowa M. , Gbadamosi B. , Benson Temitope O. , Adebimpe O. , Georgina A. L. 
Published: July 2021

AbstractLassa fever is a zoonotic disease spread by infected rodents known as multimammate rats. The disease has posed a significant and major health challenge in West African countries, including Nigeria. To have a deeper understanding of Lassa fever epidemiology in Nigeria, we present a deterministic dynamical model to study its dynamical transmission behavior in the population. To mimic the disease’s biological history, we divide the population into two groups: humans and rodents. We established the quantity known as reproduction number $${\mathcal {R}}_{0}$$

R
0

. The results show that if $${\mathcal {R}}_{0} <1$$

R
0

<
1

then the system is stable, otherwise it is unstable. The model fitting was performed using the nonlinear least square method on cumulative reported cases from Nigeria between 2018 and 2020 to obtain the best fit that describes the dynamics of this disease in Nigeria. In addition, sensitivity analysis was performed, and the numerical solution of the system was derived using an iterative scheme, the fifth-order Runge–Kutta method. Using different numeric values for each parameter, we investigate the effect of all highest sensitivity indices’ parameters on the population of infected humans and infected rodents. Our findings indicate that any control strategies and methods that reduce rodent populations and the risk of transmission from rodents to humans and rodents would aid in the population’s control of Lassa fever.
Values-based practice: how can history taking help psychiatrists explore the values involved in clinical decision-making?
Authors: Dudas Robert B. 
Published: July 2021

SUMMARYValues-based practice (VBP) is a framework of clinical theory and skills to facilitate a good process whereby the (often conflicting) values involved in clinical decision-making can be recognised and balanced productively. Many of these values come from the personal histories of the patient and of the clinician, and the traditions and history of psychiatry. New developments in science lead to increasing choice and increasing complexity of values. Therefore, psychiatrists will need more skills in this area, as reflected by the inclusion of VBP in the Royal College of Psychiatrists’ training curricula. This article describes some tools for understanding and navigating this value diversity in applying science to clinical practice during history taking.
Recommendations for monitoring and reporting harm in mindfulness for psychosis research
Authors: Ellett Lyn , Chadwick Paul 
Published: July 2021

SummaryThere is increasing interest in potential harmful effects of mindfulness-based interventions. In relation to psychosis, inconsistency and shortcomings in how harm is monitored and reported are holding back our understanding. We offer eight recommendations to help build a firmer evidence base on potential harm in mindfulness for psychosis.
Descriptive Comparative Analysis of Patients With Cancer Referring to the Emergency Department of an Italian University Hospital Across the Severe Acute Respiratory Syndrome Coronavirus 2 Waves
Authors: Mariniello Annapaola , Bironzo Paolo , Pisano Chiara , De Filippis Marco , Persano Irene , Olmetto Emanuela , Caramello Valeria , Boccuzzi Adriana , Capelletto Enrica , Passiglia Francesco , Di Maio Massimo , Novello Silvia 
Published: July 2021

PURPOSE: COVID-19 cancer patients (C19-CP) represent a population at high risk for mortality, whose clinical characteristics are still unknown in the second SARS-CoV-2 wave. The aim of this retrospective study was to compare epidemiology and clinical presentation of C19-CP referring to the emergency department (ED) of our institution (San Luigi Gonzaga University Hospital, Orbassano, Turin, Italy), in a 3-week observation period of the first and second COVID-19 waves, starting from the introduction of the corresponding national lockdowns. METHODS: We retrieved ED admissions from March 9 to 29, 2020, for the first wave, and from October 24 to November 13, 2020, for the second wave. We collected clinical characteristics of consecutive patients with molecularly confirmed SARS-CoV-2 infection. We also considered untested or SARS-CoV-2–negative cancer patients referring to the ED in the reference time frames. RESULTS: C19-CP in the second wave exceeded those in the first wave despite the nonsignificant difference (39 of 576 v 8 of 163; P = .5). Compared with nononcological patients, C19-CP were older (median age 70 years [interquartile range 61-77] v 60 years [interquartile range 45-73]; P = .02) and presented more often with ≥ 2 comorbidities (40.4% v 24.3%; P = .02). Compared with nononcological patients, in C19-CP, respiratory failure (29 of 47 v 321 of 692; P = .049) and hospitalization (37 of 47 v 363 of 692; P = .0004) were higher, with comparable frequencies across the waves. Five of 24 and 10 of 27 hospitalized cancer patients in the first and second waves developed SARS-CoV-2 infection during hospitalization. CONCLUSION: C19-CP were a vulnerable population, irrespective of the COVID-19 waves. This highlights the need to prioritize vaccinations in oncological patients to safeguard and guarantee optimal anticancer care.
Effect of bremsstrahlung on the characteristic growth length of an avalanche of runaway electrons
Authors: Oreshkin E. 
Published: July 2021

Abstract
In avalanches of runaway electrons, which are formed in high-altitude atmospheric discharges, the average electron energy is several megaelectronvolts. For such high-energy electrons, the loss of energy by bremsstrahlung is significant when they collide with gas atoms. The aim of this study was to evaluate the effect of bremsstrahlung on the exponential growth length of a runaway electron avalanche. It has been shown that taking account of the bremsstrahlung produced by the electrons of an avalanche gives corrections of no more than a few percent to the avalanche exponential growth length. The corrections are maximum for electric field strengths close to the threshold below which the avalanche electrons fail to become continuously accelerated. The effect of bremsstrahlung decreases with increasing electric field strength. The higher the atomic number of the gas in which an avalanche of runaway electrons propagates, the greater the corrections.
Pneumococcal nasopharyngeal carriage and antimicrobial susceptibility profile in children under five in southern Ethiopia
Authors: Hussen Siraj , Asnake Solomon , Wachamo Demelash , Tadesse Birkneh Tilahun 
Published: July 2021

Background: Streptococcus pneumonia causes high morbidity and mortality, particularly in children under five. Nasopharyngeal (NP) carriage predisposes individuals to pneumococcal infection and horizontal spread within the community. Overuse of antibiotics has been linked to increased risk of antimicrobial resistance to S. pneumonia. We investigated NP carriage rate and resistance to commonly prescribed antibiotics in under-five children visiting a public referral center in southern Ethiopia. Methods: In total, 413 under 5 children who visited the outpatient department for a health check-up, immunization or acute mild illnesses underwent NP sampling. Parent/caregiver surveys were administered at the clinic. Sterile plastic applicator rayon tipped swabs were used for NP sampling. Antimicrobial susceptibility testing was performed using modified the disk diffusion method. Results: S. pneumonia NP carriage was observed in 39% [95% confidence interval (CI): 34.4–43.8]. Living with one or more sibling (AOR (adjusted odds ratio) 1.95: 95% CI: 1.01, 3.76), age group of 3-23 months (AOR 2.31: 95% CI: 1.07, 4.98), co-sleeping with family (AOR 2.09, 95% CI: 1.16, 3.79), attendance at kindergarten/day-care (AOR 1.84: 95% CI: 1.09, 3.11) and malnutrition independently increased S. pneumonia carriage at the individual level. S. pneumonia was highly resistant to Oxacillin (38.5%), Tetracycline (37.3%), and Trimethoprim-sulfamethoxazole (34.2%). Multi-drug resistance was observed in 42.2% of isolates. Conclusions: A high streptococcal NP carriage rate was observed in under-five children. The high level of resistance to commonly used antibiotics calls for enhancing national surveillance of resistance patterns and enforce antibiotic stewardship efforts.
Case Report: An immunocompromised, vaccine breakthrough COVID-19 patient with pneumonia
Authors: Patrinos Antonios , Komninos Dimitris , Dimouli Katerina , Kartsonakis Andreas , Serpanos Dimitris , Kalogeropoulos Georgios , Dimitropoulou Dimitra 
Published: July 2021

Background: The novel SARS-CoV-2 pandemic has inflicted a major blow on public health worldwide accounting for millions of deaths and subsequent socio-economic consequences. The main challenge for scientists and researchers has been to restrain transmission of the virus and prevent severe respiratory disease. Novel promising vaccines aim to fulfil these expectations, although new variants of the coronavirus have emerged. The present manuscript aims to add to the knowledge, through a case of an immunodeficient patient, who developed remarkably favorable recovery from SARS-CoV-2 pneumonia after having been fully vaccinated against the novel coronavirus. Case presentation: An 82-year-old Caucasian male with a history of metastatic pancreatic cancer was admitted with signs and symptoms of pneumonia. Workup revealed a positive SARS-CoV-2 real time – polymerase chain reaction (RT-PCR) and further investigation identified a B.1.1.7 variant. The imaging essays showed extensive lung disease. Interestingly, the patient had already received the second dose of the BNT162b2 (Pfizer) vaccine against the new coronavirus 16 days prior. After having been treated with appropriate antiviral and antibiotical agents the patient showed significantly favorable recovery and no need for high oxygen flows and no complications presented. The patient was discharged after six days of hospitalization in good condition, with no need for supplementary oxygen at home. Conclusions: Despite breakthrough cases, vaccination against COVID-19 is crucial for restraining the novel coronavirus. Further studies should be carried out in order to determine the optimal strategies for large-scale vaccination while minimizing the risk of further and faster evolution of the virus.
Fecal calprotectin as a predictor of gastrointestinal immune-related adverse events (CF-19): A prospective study.
Authors: Cardeña Gutiérrez Ana , Mielgo Rubio Xabier , Ruiz Muñoz Manuel , Martinez Cabañes Ruth , Moreno Muñoz Diana , Hernando Polo Susana , Olier Garate Clara , Hurtado Nuño Alicia , Sotelo Peña Verónica , Sánchez Becerra Maria Virginia , González López Andrea María , Esteban García Mónica , Robles Bermejo Teresa , Pérez Fernández Elia , Cámara Vicario Juan Carlos 
Published: July 2021

Background: Colitis is a frequent immune-related toxicity, without any biomarker that may predict its onset. It is endoscopically similar to intestinal inflammatory diseases, where fecal calprotectin (FC) is used as a biomarker to early-detect a relapse. We found contradictory evidence about FC and immunotherapy and no prospective study was already published. Methods: We present an analytical, observational and prospective study of one year’s duration. We analyzed FC basal, and then prior to each cycle until the sixth, ending with quarterly follow-up. For evaluating the predictive value of FC we estimated the area under the ROC curve for basal absolute values and for each cycle, and calculated its relative percentage change with respect to basal. We also planned to estimate sensitivity, specificity and predictive values indexes for different cut-off points. Because of lack of recruitment we did a preliminary analysis at the end of the initially estimated period before suggesting its prolongation. Results: 24 patients (19 male) were included in the study. This included n=15 diagnosed with lung cancer, head and neck, renal, bladder and colorectal cancer (n=2, each), and melanoma (n=1). They were treated with Anti PD-1/PDL-1 mono therapy (n=18), combo with chemo (n=2), or combo with anti-CTLA4 (n=2). Three patients had G1 colitis and two, >=G2, all treated with anti-PD1 and before 6th cycle, as described on literature. ROC curve presents AUC 0,559 (CI95%:0,32-0,798) and RR for colitis taking FC value is 1,001 for each 10 units (p=0,493). Conclusion: Even though we must take into account the limitations of the study we cannot conclude that FC could be used as a predictor for detecting immune-mediated colitis.
Andrographolide, isolated from Andrographis paniculata, induces apoptosis in monocytic leukemia and multiple myeloma cells via augmentation of reactive oxygen species production
Authors: Doi Hiroki , Matsui Taei , Dijkstra Johannes M. , Ogasawara Atsushi , Higashimoto Yuki , Imamura Seiji , Ohye Tamae , Takematsu Hiromu , Katsuda Itsuro , Akiyama Hidehiko 
Published: July 2021

Background: Andrographolide (Andro) is a diterpenoid component of the plant Andrographis paniculata that is known for its anti-tumor activity against a variety of cancer cells. Methods: We studied the effects of Andro on the viability of the human leukemia monocytic cell line THP-1 and the human multiple myeloma cell line H929. Andro was compared with cytosine arabinoside (Ara-C) and vincristine (VCR), which are well-established therapeutics against hematopoietic tumors. Results: Andro reduced the viability of THP-1 and H929 in a dose-dependent manner. H929 viability was highly susceptible to Andro, although only slightly susceptible to Ara-C. The agents Andro, Ara-C, and VCR each induced apoptosis, as shown by cellular shrinkage, DNA fragmentation, and increases in annexin V-binding, caspase-3/7 activity, reactive oxygen species (ROS) production, and mitochondrial membrane depolarization. The apoptotic activities of Andro were largely suppressed by N-acetyl-L-cysteine (NAC), an inhibitor of ROS production, whereas NAC hardly affected the apoptotic activities of Ara-C and VCR. Furthermore, whereas Ara-C and VCR increased the percentages of cells in the G0/G1 and G2/M phases, respectively, Andro showed little or no detectable effect on cell cycle progression. Conclusions: Andro induces ROS-dependent apoptosis in monocytic leukemia THP-1 and multiple myeloma H929 cells, underlining its potential as a therapeutic agent for treating hematopoietic tumors. Notably, the high sensitivity of H929 cells is encouraging for further studies on the use of Andro against multiple myeloma.
The number of osteoblasts and osteoclasts in hypofunctional teeth during orthodontic tooth movement in rats
Authors: Maulani Adibah , Farmasyanti Cendrawasih Andusyana , Sutantyo Darmawan 
Published: July 2021

Background: When moved orthodontically, hypofunctional teeth will have a decreased tooth movement rate compared to normal teeth. This study aimed to determine the number of osteoblasts in the tension side and the number of osteoclasts in the pressure side of the hypofunctional teeth during orthodontic tooth movement. Method: 18 male Wistar rats were given a palatal coil spring application on the maxillary incisors. Rats were divided into two groups, the orthodontic group with normal occlusion (NO) and hypofunctional occlusion (HO). The number of osteoblasts on the tension side and osteoclasts on the pressure side on days zero (D0), five (D5), and 10 (D10) were tested with two-way ANOVA. Observations were made by hematoxylin eosin staining. Result: The results showed that the number of osteoblasts on the tension side of the HO group was the same at the NO group (p> 0.05). The number of osteoblasts on the tension side in the NO and HO groups at D5 was the same at D10 (p = 0.99), but significantly higher (p = 0.002), than D0. The number of osteoclasts on the pressure side in the HO group was significantly lower than the NO group (p <0.05). The number of osteoclasts in the NO D5 group was significantly higher than the other groups (p <0.05). Conclusions: The number of osteoblasts on the tension side was not affected by the hypofunctional state but decreased the number of osteoclasts on the pressure side during orthodontic tooth movement.