bluuebunny/crossref_metadata_2025_split huggingface dataset:
Assistência multidisciplinar na prevenção de infecção do trato urinário associada à cateter vesical de demora em UTI: scoping review
Authors: Santos Junior Paulo Sérgio , Santos Cibelle Ferreira Hilário dos , Borges Junior Adenilson Crisostomo , Nascimento Júlio César Coelho do 
Published: September 2021

Objetivo: mapear a produção cientifica acerca da atuação multidisciplinar no controle de infecção do trato urinário associada a cateter vesical de demora (ITU-AC) em Unidade de Terapia Intensiva (UTI). Método: Trata-se de uma Scoping Review elaborado conforme recomendação do instituto Joanna Briggs (JBI) e de acordo como quadro teórico recomendado por Arkseye O’Malley. A questão que norteou a pesquisa foi baseada na estratégia PCC (População, Conceito e Contexto). Sendo P: Estudos com pacientes submetidos ao cateter vesical de demora em UTI. C: Intervenções relacioandas a redução da ITU-AC. C: UTI. Foram consultadas as bases de dados: Embase®, Scopus e Biblioteca Virtual em Saúde (BVS). Para manter a coerência na busca dos artigos, os descritores e as palavras-chaves foram utilizados isoladamente e associados, de acordo com as características específicas de cada uma das bases de dados consultadas. Foram incluídos estudos publicados entre 2012 e 2021 sem restrição de idiomas. Resultados: Foram encontrados 157 artigos nas buscas nas bases de dados, no entanto, apenas 19 responderam a pergunta de pesquisa. Os resultados apontaram que a equipe multiprofissional deve implementar estratégias de controle das IRAS, pois significativamente reduzem a taxa de ITU-AC, realizar programas de treinamentos profissional, envolver os profissionais de saúde na unidade, as rodadas da equipe interdisciplinar têm impacto diretamente na redução dos dias de uso do Cateter Vesical de Demora (CVD). Conclusão: A equipe multidisciplinar necessita adotar atitudes como a capacitação dos profissionais, ultilizar da técnica correta de inserção do CVD e a sua manutenção, incentivar a importância da lavagem das mãos, revezamento nos atendimentos das equipes multiprofissionais, pois estão ligadas diretamente aos riscos e benefícios do procedimento de CVD direcionando assim a individualização dos cuidados multidisciplinares.
Fake News – o caso do cozimento micro-ondas
Authors: Zanatta Shalimar Calegari , Santos Hudson Dourado dos , Carvalho Hercília Alves de Pereira , Zanatta Alisson Calegari 
Published: September 2021

As informações veiculadas livremente pelas mídias sociais não apresentam compromisso com a ética, a moral ou com quaisquer princípios teóricos ou científicos. Estas informações podem se tornar um problema social grave quando utilizam conteúdos falsos para manipular a opinião pública. Se considerarmos que a democracia e a liberdade se constroem com indivíduos que fazem escolhas conscientes, as fake News, como ficaram conhecidas, devem ser combatidas. Neste contexto, este artigo apresenta e discute, á luz do conhecimento científico, as principais informações veiculadas, sobre o cozimento micro-ondas entre agosto de 2020 a fevereiro de 2021 pelas mídias sociais como proposta de combate às fakes News. Os resultados mostram que classificar uma notícia como falsa ou verdadeira não é uma tarefa simples. Isto porque as notícias fakes, tentam sensibilizar o leitor e reforçam ideias do senso comum. Assim, defendemos a alfabetização científica, como resultado de um processo ensino aprendizagem eficiente, como possível caminho para o combate às fake News.
Relação entre aleitamento e desenvolvimento do sistema estomatognático: revisão sistemática
Authors: Bernardo Graziela Mackowiesky Brigido , Gonçalves Laura Faustino , Haas Patrícia , Blanco-Dutra Ana Paula 
Published: September 2021

Introdução: A amamentação promove benefícios, tanto para o recém nascido, quanto para a mãe. Apesar da importância do aleitamento materno, há dificuldades peculiares para a mãe e o bebê no período de internação, podendo levar ao desmame precoce. Objetivo: Verificar a relação entre aleitamento e os reflexos na motricidade orofacial. Metodologia: A busca por artigos científicos foi conduzida por dois pesquisadores independentes nas bases de dados MEDLINE (Pubmed), LILACS, SciELO, SCOPUS, WEB OF SCIENCE e BIREME, sem restrição de idioma e localização, durante o período de 2010 a 2021. Para complementar e evitar viés de risco foi realizada uma busca por literatura cinza no Google Scholar. A revisão sistemática foi conduzida conforme as recomendações do Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA). Foram inclusos na pesquisa estudos que obtiveram pontuação ≥ a 12 pontos segundo o protocolo para pontuação qualitativa proposto por Pithon et al. (2015). Resultados: A duração do aleitamento materno associou-se positivamente à qualidade da função mastigatória. Os estudos mostraram que a amamentação tem um impacto positivo na função mastigatória e de sucção. Conclusão: Ao relacionar os achados desta pesquisa com o desmame precoce e seus reflexos no sistema estomatognático evidencia-se que o aleitamento materno natural oferece ao bebê um adequado desenvolvimento ósseo e muscular, garantindo, assim, o perfeito funcionamento das estruturas orais e possibilitando saúde geral devido às interrelações existentes entre o sistema estomatognático e os demais órgãos e funções.
Optimization of cone beam computed tomography for the assessment of alterations of the maxillary sinuses
Authors: Anrain Bárbara Cristina , Franco Ademir , Brasil Danieli Moura , Junqueira José Luiz Cintra , Oliveira Luciana Butini de , Oenning Anne Caroline Costa 
Published: September 2021

Objective: To test the standard protocols of a CBCT unit in order to find lower-dose alternatives with diagnostically acceptable image quality for the maxillary sinuses visualization. Study design: An observational study was performed. Two dry skulls were used to simulate four conditions of the maxillary sinuses: normality, mucous retention pseudocyst, membrane thickening and bone graft. Cone beam computed tomography scans were obtained with an i-CAT classic unit using different acquisition protocols and a box of polystyrene to simulate soft tissue attenuation. All the protocols were established by the manufacturer, combining different energy parameters, fields of view and voxel sizes. Multiplanar reconstructions were presented to three Oral Radiologists through blind and randomized distribution. The specialists judged general image quality, sharpness, contrast, and the presence of noise and artifacts based on a 4-points scale. Results: Protocols with higher energy parameters had significant association with higher scores for general quality, sharpness and contrast (p<0.05). Protocols with intermediate level of radiation dose had also significant association with good and excellent image quality; for the presence of noise and artifacts the images were rated acceptable. Conclusion: i-CAT default protocols with lower dose of radiation were able to deliver acceptable image quality for the visualization of the maxillary sinuses.
The Fabrication of a Gellan Gum-Based Hydrogel Loaded With Magnesium Ions for the Synergistic Promotion of Skin Wound Healing
Authors: Li Wenqiang , Jian Xingling , Zou Yanfen , Wu Lin , Huang Haiyan , Li Hui , Hu Dandan , Yu Bo 
Published: September 2021

To accelerate serious skin burn wound healing in a convenient manner, an interpenetrating network of hydrogel consisting of gellan gum and polyacrylamide was synthesized by chemical crosslinking and Mg2+ ion immersion techniques. The prepared Mg2+@PAM/GG hydrogel was characterized by morphology, water vapor loss, swelling ratio, rheological properties, tensile mechanical, biocompatibility, and flow cytometry study. The results show that Mg2+@PAM/GG hydrogel’s mechanical strength could be enhanced by the dual network structure and physical crosslinking agent Mg2+ ions. In addition, the tension strength of Mg2+@PAM/GG hydrogel is obviously increased from 86 to 392 kPa, the elongation at break increased from 84 to 231%, and crosslinking density N increased from 4.3 to 7.2 mol/m3 compared with pure GG hydrogel. The cumulative release curve of Mg2+ ions shows that the multiple release mechanism of Mg2+ ions belong to non-Fick’s diffusion. Meanwhile, in vitro experiments show that Mg2+@PAM/GG double network hydrogel has favorable proliferation and an NF-κB pathway inhibition property for fibroblast cells. Finally, the healing effect of the Mg2+@PAM/GG was evaluated in a rat full-thickness burn model. The animal study demonstrates that Mg2+@PAM/GG could accelerate the healing efficiency in case of the sustained-released Mg2+ ions in wound beds. Considering this excellent performance, this convenient prepared hydrogel has great potential as a commercial application for skin full-thickness burn healing materials.
A Comprehensive Analysis of SE-lncRNA/mRNA Differential Expression Profiles During Chondrogenic Differentiation of Human Bone Marrow Mesenchymal Stem Cells
Authors: Jiang Yu , Zhang Chen , Long Lujue , Ge Lihua , Guo Jing , Fan Zhipeng , Yu Guoxia 
Published: September 2021

Objective: Articular cartilage injury is common and difficult to treat clinically because of the characteristics of the cartilage. Bone marrow-derived mesenchymal stem cell (BMSC)-mediated cartilage regeneration is a promising therapy for treating articular cartilage injury. BMSC differentiation is controlled by numerous molecules and signaling pathways in the microenvironment at both the transcriptional and post-transcriptional levels. However, the possible function of super enhancer long non-coding RNAs (SE-lncRNAs) in the chondrogenic differentiation of BMSCs is still unclear. Our intention was to explore the expression profile of SE-lncRNAs and potential target genes regulated by SE-lncRNAs during chondrogenic differentiation in BMSCs.Materials and Methods: In this study, we conducted a human Super-Enhancer LncRNA Microarray to investigate the differential expression profile of SE-lncRNAs and mRNAs during chondrogenic differentiation of BMSCs. Subsequent bioinformatic analysis was performed to clarify the important signaling pathways, SE-lncRNAs, and mRNAs associated with SE-lncRNAs regulating the chondrogenic differentiation of BMSCs.Results: A total of 77 SE-lncRNAs were identified, of which 47 were upregulated and 30 were downregulated during chondrogenic differentiation. A total of 308 mRNAs were identified, of which 245 were upregulated and 63 were downregulated. Some pathways, such as focal adhesion, extracellular matrix (ECM)–receptor interaction, transforming growth factor-β (TGF-β) signaling pathway, and PI3K–Akt signaling pathway, were identified as the key pathways that may be implicated in the chondrogenic differentiation of BMSCs. Moreover, five potentially core regulatory mRNAs (PMEPA1, ENC1, TES, CDK6, and ADIRF) and 37 SE-lncRNAs in chondrogenic differentiation were identified by bioinformatic analysis.Conclusion: We assessed the differential expression levels of SE-lncRNAs and mRNAs, along with the chondrogenic differentiation of BMSCs. By analyzing the interactions and co-expression, we identified the core SE-lncRNAs and mRNAs acting as regulators of the chondrogenic differentiation potential of BMSCs. Our study also provided novel insights into the mechanism of BMSC chondrogenic and cartilage regeneration.
Adrenal Myelolipoma: 369 Cases From a High-Volume Center
Authors: Lin Lede , Gong Lina , Cheng Liang , Liu Zhihong , Shen Sikui , Zhu Yuchun , Zhou Liang 
Published: September 2021

Background: Adrenal myelolipoma (AML) is a nonfunctional benign neoplasm from the adrenal cortex, composed of mature fat and hematopoietic tissue. Usually, patients have no symptoms. However, some patients with hypertension and blood pressure normalize after AML surgery, indicating some connections between AML and hypertension.Materials and Methods: This was a retrospective cohort study of 369 patients diagnosed with AML from September 2008 to December 2018 collected in the Urology Department of West China Hospital, Chengdu, Sichuan, China. We collected clinical records of patients before surgery. Postoperative follow-up was also carried out for those with hypertension and whether patients needed to take antihypertensive drugs and postoperative blood pressure were recorded. We aim to explore the characteristics of both patients with AML having hypertension and having remission of hypertension in 1 year after surgery.Results: There were 369 patients with AML included in the study, 156 men and 213 women, aged 49.86 ± 11.61 years old. Among them, 121 (32.8%) patients presented with hypertension. Body mass index was significantly higher in the hypertension group than that in the nonhypertension group, even after adjusting other variables (26.26 ± 3.43 vs. 24.28 ± 3.38 kg/m2, P &lt; 0.001 for both univariate and multivariate analyses). Sixty patients were followed up for 1–9 years, with a median follow-up of 52 months. The duration of hypertension in the remission group was shorter than that in the non-remission group (P = 0.020), and the tumor lateralization was significantly different between the two groups (P = 0.005).Conclusions: Nearly one-third of patients with AML suffered from hypertension in our study, and there existed some potential links between AML and hypertension. To be more specific, AML-related hypertension was more likely to result from obesity and renal compression by perirenal fat than from endocrine disorders or blood vessels compression. Patients with AML and with more than 3 years of hypertension might have less possibility to recover.
Identification of Signature Genes Associated With Invasiveness and the Construction of a Prognostic Model That Predicts the Overall Survival of Bladder Cancer
Authors: He Yang , Wu Yongxin , Liu Zhe , Li Boping , Jiang Ning , Xu Peng , Xu Abai 
Published: September 2021

Background: Bladder cancer has become the tenth most diagnosed cancer worldwide. The prognosis has been shown to differ between non-muscle invasive bladder cancer (NMIBC) and muscle invasive bladder cancer (MIBC). We aimed to identify signature genes that are associated with the invasiveness and survival of bladder cancer and to identify potential treatments.Methods: We downloaded gene expression profiles of bladder cancer from the Gene Expression Omnibus database to identify differentially expressed genes and perform weighted gene co-expression network analysis. Functional enrichment was analyzed by GO and KEGG analyses. Hub genes were identified from the significant module. Another dataset was also acquired to verify the expression of hub genes. Univariate and multivariate Cox regression analyses were applied to the dataset downloaded from The Cancer Genome Atlas database. Risk scores were calculated and the effect was evaluated by Kaplan-Meier survival analysis. A nomogram was constructed and validated using training and testing samples, respectively. Analysis of the tumor immune microenvironment was conducted with the CIBERSORT algorithm.Results: In total, 1,245 differentially expressed genes (DEGs) were identified. A distinct module was identified that was significantly correlated to invasiveness. The genes within this module were found to be significantly associated with extracellular exosomes, GTPase activity, metabolic pathways, etc. Three hub genes (VSIG2, PPFIBP2, and DENND2D) were identified as biomarkers of invasiveness; two of these (PPFIBP2 and DENND2D) were closely associated with prognosis. The risk score was regarded as an independent prognostic factor. The nomogram was associated with acceptable accuracy for predicting 1- and 5-year overall survival. The infiltrating levels of resting NK cells, activated natural killer (NK) cells, CD8+ T cells, activated memory CD4+ T cells, and T follicular helper cells, were significantly higher in the group with lower risk scores. The group with higher risk scores showed predominant infiltration by regulatory T cells (Tregs).Conclusion: We successfully identified three signature genes related to invasiveness and constructed a nomogram of bladder cancer with acceptable performance. Differences suggested by risk scores between groups of patients showing diverse patterns of immune cell infiltration may be beneficial for selecting therapeutic approaches and predicting prognosis.
Time Course Transcriptomic Study Reveals the Gene Regulation During Liver Development and the Correlation With Abdominal Fat Weight in Chicken
Authors: Xing Siyuan , Liu Ranran , Zhao Guiping , Groenen Martien A. M. , Madsen Ole , Liu Lu , Zheng Maiqing , Wang Qiao , Wu Zhou , Crooijmans Richard P. M. A. , Wen Jie 
Published: September 2021

Background:The liver is the central metabolic organ of animals. In chicken, knowledge on the relationship between gene expression in the liver and fat deposition during development is still limited. A time-course transcriptomic study from the embryonic (day 12) to the egg-producing period (day 180 after hatch) was performed to profile slow-growing meat type chicken liver gene expression and to investigate its correlation with abdominal fat deposition.Results:The transcriptome profiles showed a separation of the different developmental stages. In total, 13,096 genes were ubiquitously expressed at all the tested developmental stages. The analysis of differentially expressed genes between adjacent developmental stages showed that biosynthesis of unsaturated fatty acids pathway was enriched from day 21 to day 140 after hatch. The correlation between liver gene expression and the trait abdominal fat weight (AFW) was analyzed by weighted gene co-expression network analysis. The genesMFGE8,HHLA1,CKAP2, andACSBG2were identified as hub genes in AFW positively correlated modules, which suggested important roles of these genes in the lipid metabolism in chicken liver.Conclusion:Our results provided a resource of developmental transcriptome profiles in chicken liver and suggested that the geneACSBG2among other detected genes can be used as a candidate gene for selecting low AFW chickens.
Functional Exhaustion of HBV-Specific CD8 T Cells Impedes PD-L1 Blockade Efficacy in Chronic HBV Infection
Authors: Ferrando-Martinez Sara , Snell Bennett Angie , Lino Elisabete , Gehring Adam J. , Feld Jordan , Janssen Harry L. A. , Robbins Scott H. 
Published: September 2021

BackgroundA functional cure for chronic HBV could be achieved by boosting HBV-specific immunity. In vitro studies show that immunotherapy could be an effective strategy. However, these studies include strategies to enrich HBV-specific CD8 T cells, which could alter the expression of the anti-PD-1/anti-PD-L1 antibody targets. Our aim was to determine the efficacy of PD-L1 blockade ex vivo.MethodsHBV-specific CD8 T cells were characterized ex vivo by flow cytometry for the simultaneous analysis of six immune populations and 14 activating and inhibitory receptors. Ex vivo functionality was quantified by ELISpot and by combining peptide pool stimulation, dextramers and intracellular flow cytometry staining.ResultsThe functionality of HBV-specific CD8 T cells is associated with a higher frequency of cells with low exhaustion phenotype (LAG3-TIM3-PD-1+), independently of the clinical parameters. The accumulation of HBV-specific CD8 T cells with a functionally exhausted phenotype (LAG3+TIM3+PD-1+) is associated with lack of ex vivo functionality. PD-L1 blockade enhanced the HBV-specific CD8 T cell response only in patients with lower exhaustion levels, while response to PD-L1 blockade was abrogated in patients with higher frequencies of exhausted HBV-specific CD8 T cells.ConclusionHigher levels of functionally exhausted HBV-specific CD8 T cells are associated with a lack of response that cannot be restored by blocking the PD-1:PD-L1 axis. This suggests that the clinical effectiveness of blocking the PD-1:PD-L1 axis as a monotherapy may be restricted. Combination strategies, potentially including the combination of anti-LAG-3 with other anti-iR antibodies, will likely be required to elicit a functional cure for patients with high levels of functionally exhausted HBV-specific CD8 T cells.
Safety and Preliminary Efficacy of a Novel Host-Modulatory Therapy for Reducing Gingival Inflammation
Authors: Hasturk Hatice , Schulte Fabian , Martins Melissa , Sherzai Homa , Floros Constantinos , Cugini MaryAnn , Chiu Chung-Jung , Hardt Markus , Van Dyke Thomas 
Published: September 2021

BackgroundPeriodontal disease is among the sixth most common inflammatory diseases worldwide with high risk to promote complications from other inflammatory diseases including diabetes, cardiovascular disease and Alzheimer’s Disease. Failure of active resolution of inflammation pathways is implicated in pathogenesis of periodontal diseases, including gingivitis. Lipoxin A4 (LXA4), a member of the specialized pro-resolving lipid mediators (SPMs) that drive resolution of inflammation via GPC-receptor mediated pathways, offered therapeutic advantages in preclinical models of periodontitis.MethodsWe conducted a randomized, placebo-controlled, parallel-group Phase 1 clinical trial to determine the safety and preliminary efficacy of an LXA4 analog in patients with gingival inflammation. One hundred twenty-seven (127) individuals were randomized to daily use of an oral rinse containing a LXA4 mimetic, methyl ester-benzo-lipoxin A4 (BLXA4), placebo rinse or a no-rinse control group for 28 days. Treatment emergent adverse events (TEAEs) were assessed for safety, the primary outcome. Secondary outcomes included the change in the level of gingival inflammation and periodontal pocket depth (PD). Serum SPMs were monitored using targeted lipid mediator lipidomics to assess potential systemic impact of BLXA4.ResultsThe frequency of TEAEs was similar in BLXA4 and placebo-treated groups with no study-related SAEs. Once-daily rinsing with BLXA4 for 28-days resulted in a greater decrease in gingival inflammation compared to placebo rinse and no-rinse control groups (mean change: 0.26 GI unit vs 0.21 and 0.17, respectively). PD reduction was also greater with BLXA4 oral rinse compared to placebo and no-rinse groups (mean reduction: 1.23 mm vs. 0.71 mm and 0.46 mm, respectively). Topical application of BLXA4 increased serum levels of SPMs.ConclusionTreatment with BLXA4 reduces local inflammation, and increases abundance of pro-resolution molecules systemically, which may dampen inflammation that can mediate progression and course of inflammatory diseases beyond periodontitis.Clinical Trial RegistrationClinicalTrials.gov, identifier (NCT02342691).