bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Novel Hypoxic lnc-RNA, HRL-SC, Promotes Proliferation and Migration of Human Dental Pulp Stem Cells Through PI3K/AKT Signalling Pathway
Authors: Zeng Junkai , Chen Ming , Yang Yeqing , Wu Buling 
Published: December 2021

Abstract
Background: Human dental pulp stem cells (hDPSCs) are critical for pulp generation. hDPSCs proliferate faster under hypoxia, but the regulatory mechanism of long noncoding RNAs (lncRNAs) in this process is not fully understood.Methods: Novel lncRNAs were obtained by reanalysis of transcriptome datasets coming from RNA-Seq under hypoxia compared with normoxia, and differential expression analysis of target genes were performed. Bioinformatics analyses including Gene Ontology (GO) analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis and Gene Set Enrichment Analysis (GSEA) analysis were used to understand the function of key novel lncRNA. hDPSCs were isolated from dental pulp tissue. EdU test and scratch healing test were used to detect the proliferation and migration of hDPSCs. qRT-PCR was used to detect the RNA level expression changes of selected genes. RNA fluorescence in situ hybridization (FISH), small interfering RNA (siRNA), qRT-PCR and western blot analysis were used to explore the function of key novel lncRNA. Results: We identified 496 novel lncRNAs in hDPSCs under hypoxia, including 45 expressed differentially novel lncRNAs. Of them, we focused on a key novel lncRNA, which we named HRL-SC (hypoxia related lncRNA in stem cells). Functional annotation revealed that HRL-SC was associated with hypoxic conditions and PI3K/AKT signalling pathway. HRL-SC was mainly located in the cytoplasm of hDPSCs and had stably high expression under hypoxia. Knockdown of HRL-SC inhibited proliferation and migration of hDPSCs and expression levels of PI3K/AKT related marker proteins. Furthermore, AKT activator SC79 partially offset the inhibitory effect caused by the knockdown, indicating that HRL-SC promoted hDPSCs through PI3K/AKT signalling pathway.Conclusion: Hypoxia related lncRNA HRL-SC promotes proliferation and migration of hDPSCs through PI3K/AKT signalling pathway and it may provide a better understanding for regenerative application of hDPSCs.
CircRNA_0263 and circRNA_1507 are Dysregulated in Atrial Fibrosis Rat Induced by Chronic Intermittent Hypoxia
Authors: Liang Xue , Liu Yanhong , Liu Yu , Wang Weiding , Shangguan Wenfeng , Wang Manman , Zhang Xiaowei , Miao Shuai , Liu Enzhao , Li Guangping 
Published: December 2021

Abstract
Aims: This study aimed to characterize circular RNA (circRNA) profiles associated with atrial fibrosis and identify critical circRNAs in a rat model of atrial fibrosis.Methods: Sprague Dawley rats were randomly divided into control and atrial fibrosis groups (n=15 in each group). For rats in the atrial fibrosis group, atrial fibrosis was induced by chronic intermittent hypoxia. Atrial tissues were isolated for circRNA sequencing. The dysregulated circRNAs in atrial fibrosis were identified by DESeq. Subsequently, the potential functions of circRNAs in atrial fibrosis were investigated by Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analysis of the host genes. The interactions among circRNAs, microRNAs (miRNAs), and mRNAs were analyzed by constructing a competing endogenous RNA (ceRNA) network. Finally, the crucial circRNAs were validated by quantitative real-time polymerase chain reaction (qRT-PCR).Results: Five upregulated and 11 downregulated circRNAs were identified in the atrial fibrosis group. These dysregulated circRNAs were primarily associated with “carbohydrate metabolism” and “cardiovascular diseases.” Two circRNAs (circRNA_0263 and circRNA_1507) were able to regulate target gene expression by interacting with corresponding miRNAs, including rno-miR-29b-5p, rno-miR-29b-3p, rno-miR-496-5p, rno-miR-136-5p, and novel123-mature. qRT-PCR successfully validated the differential expression of circRNA_0263 and circRNA_1507.Conclusion: A series of circRNAs were identified as dysregulated in an atrial fibrosis rat model. The dysregulation of two circRNAs (circRNA_0263 and circRNA_1507) might be crucial for atrial fibrosis development by competing with several miRNAs.
Cistrome And Transcriptome Analysis Identifies Unique Androgen Receptor (AR) And AR- V7 Splice Variant Chromatin Binding And Transcriptional Activities
Authors: Basil Paul , Robertson Matthew J. , Bingman William E. , Dash Amit K. , Krause William C. , Shafi Ayesha A. , Piyarathna Badrajee , Coarfa Cristian , Weigel Nancy L. 
Published: December 2021

Abstract
The constitutively active androgen receptor (AR) splice variant, AR-V7, plays an important role in resistance to androgen deprivation therapy in castration resistant prostate cancer (CRPC). Studies seeking to determine whether AR-V7 is a partial mimic of the AR, or also has unique activities, and whether the AR-V7 cistrome contains unique binding sites have yielded conflicting results. One limitation in many studies has been the low level of AR variant compared to AR. Here, LNCaP and VCaP cell lines in which AR-V7 expression can be induced to match the level of AR, were used to compare the activities of AR and AR-V7. The two AR isoforms shared many targets, but overall had distinct transcriptomes. Optimal induction of novel targets sometimes required more receptor isoform than classical targets such as PSA. The isoforms displayed remarkably different cistromes with numerous differential binding sites. Some of the unique AR-V7 sites were located proximal to the transcription start sites (TSS). A de novo binding motif similar to a half ARE was identified in many AR-V7 preferential sites and, in contrast to conventional half ARE sites that bind AR-V7, FOXA1 was not enriched at these sites. This supports the concept that the AR isoforms have unique actions with the potential to serve as biomarkers or novel therapeutic targets.
OPEN High Gain Low-Cost Antenna Based on the Utilization of Diffracted Fields from Semi-Ring Shape Dielectric Edges
Authors: Al-Alem Yazan , Sifat Syed M. , Antar Yahia M.M. , Kishk Ahmed A. 
Published: December 2021

Abstract
A simple antenna with a 20-dBi gain is proposed. A thorough analysis of the propagation mechanism accompanied by a unique physical insight is provided. The realized structure has a low profile, low-cost, and compact features, a detailed link to the Fresnel-Huygens principle is provided.
Time-Series prediction for the epidemic trends of COVID-19 using Conditional Generative adversarial Networks Regression on country-wise case studies
Authors: Bej Arnabi , Maulik Ujjwal , Sarkar Anasua 
Published: December 2021

Abstract
Probabilistic Regression is a statistical technique and a crucial problem in the machine learning domain which employs a set of machine learning methods to forecast a continuous target variable based on the value of one or multiple predictor variables. COVID-19 is a virulent virus that has brought the whole world to a standstill. The potential of the virus to cause inter human transmission makes the world a dangerous place. This thesis predicts the upcoming circumstances of the Corona virus to subside its action. We have performed Conditional GAN regression to anticipate the subsequent Covid-19 cases of 5 countries. The GAN variant CGAN is used to design the model and predict the Covid-19 cases for three months ahead with least error for the dataset provided. Each country is examined individually, due to their variation in population size, tradition, medical manage- ment, preventive measures. The analysis is based on confirmed data, as provided by the World Health Organization. This paper investigates how conditional Generative Adversarial Networks (GANs) can be used to accurately exhibit intricate conditional distributions. GANs have got spectacular achievement in producing convoluted highdimensional data, but work done on their use for regression prob- lems is minimal. This paper exhibits how conditional GANs can be employed in probabilistic regression. It is shown that conditional GANs can be used to evaluate a wide range of various distributions and be competitive with existing probabilistic regression models.
WITHDRAWN: Relationships Between Serum α-Klotho Concentration and Inflammation-Related Cytokines in Hemodialysis Patients
Authors: Lisowska Katarzyna A. , Storoniak Hanna , Soroczyńska-Cybula Monika , Maziewski Mateusz , Dębska-Ślizień Alicja 
Published: December 2021

Abstract
We investigated the relationship between α-Klotho and cytokines related to inflammation in HD patients. We analyzed levels of α-Klotho with ELISA and inflammatory cytokines with CBA in the serum of HD patients. There was a significant negative correlation between the concentration of serum α-Klotho and patients’ age and the serum concentration of PTH. No correlation has been found between α-Klotho and Ca or Pi. HD time, creatynine or eGFR. However, there were significant positive correlations between the concentration of α-Klotho and the serum concentration of IL-12p70, IL-10, and IL-1β. Furthermore, the concentration of IL-10 and IL-1β was significantly lower in HD patients with low α-Klotho concentrations compared with HD patients with high α-Klotho. However, in a multivariable linear regression analysis, only patients’ age was associated independently with α-Klotho level. While these results draw our attention to potential relationships between α-Klotho proteins and inflammatory markers in HD patients, our cross-sectional study could not fully explain the pathogenic link between α-Klotho and inflammation in these patients. Therefore, further studies are necessary to clarify these relationships. However, this observation aligns with previous studies that confirm a significant relationship between Klotho concentration and human aging.
Analysis of the Safety and Pregnant Outcomes of Fertility-Sparing Surgery in Ovarian Malignant Sex Cord-Stromal Tumors: A Multicenter Retrospective Study
Authors: Li Junting , Chu Ran , Chen Gang , Shen Yuanming , Lou Yanhui , Sun Chaoyang , Li Kezhen , Song Li , Qin Tianyu , Li Jingnan , Yin Yifan , Chen Zhongshao , Liu Penglin , Song Kun , Li Li , Kong Beihua 
Published: December 2021

Abstract
Background: To assess the difference in survival between fertility-sparing surgery (FSS) and radical surgery (RS) and explore pregnant outcomes after FSS in stage I malignant sex cord-stromal tumors (MSCSTs).Methods: We performed a multicenter retrospective cohort study on patients who were diagnosed with stage IA or IC MSCSTs. Inverse Probability of Treatment Weighting was performed between the FSS and RS groups. The Chi-square test and Kaplan-Meier method were used to compare the categorical variables and disease-free survival (DFS). The binary logistic regression analysis and Cox proportional hazards regression analysis were used to identify high-risk factors related to DFS and pregnancy.Results: A total of 107 patients were included, of whom 54 (50.5%) women underwent FSS, and 53 (49.5%) women underwent RS. After IPTW, 208 patients were obtained, and all of the covariates were well balanced. After a median follow-up time of 50 months (range 7-156 months), there was no significant difference of DFS between the two groups in both unweighted cohort (P=0.969) or weighted cohort (P=0.792). In the weighted cohort, stage IC (P=0.014), tumor diameter >8 cm (P=0.003), incomplete staging surgery (P=0.003) and no adjuvant chemotherapy (P <0.001) were 4 high-risk factors associated with a shorter DFS. Among 14 patients who had pregnancy desire, 11 (78.6%) women conceived successfully, and the live birth rate was 76.9%. In univariate analysis, only adjuvant chemotherapy (P=0.009) was associated with infertility.Conclusions: On the premise of complete staging surgery, FSS is safe and feasible in stage IA and IC MSCSTs with satisfactory reproductive outcomes.
Determinants of Health-related Quality of Life in Recently Detoxified Patients With Severe Alcohol Use Disorder
Authors: Lahbairi Najlaa , Laniepce Alice , Segobin Shailendra , Cabé Nicolas , Boudehent Céline , Vabret François , Rauchs Géraldine , Pitel Anne-Lise 
Published: December 2021

Abstract
Background. Health-related quality of life (HRQoL) is an important clinical outcome in Alcohol Use Disorder (AUD) and is considered as a relevant indicator of treatment success. While a better understanding of the factors affecting HRQoL would enable to adjust patients’ care to favour treatment outcome, the determinants of HRQoL in AUD remain unclear. This study aims at describing HRQoL in AUD patients and at identifying its best predictors. Methods. We included 53 recently detoxified patients with severe AUD (sAUD) and 38 healthy controls (HC). They underwent a cognitive assessment and filled in questionnaires concerning socio-demographics, alcohol history, sleep quality, depression, anxiety and impulsivity. Additionally, a HRQoL questionnaire especially designed for AUD patients (Alcohol Quality of Life Scale; AQoLS) was used.Results. sAUD patients reported that alcohol affects their HRQoL mainly in the “negative emotions”, “control”, “relationships”, and “sleep” domains. Compared to HC, they were impaired on episodic memory, working memory, executive functions, and processing speed tasks. They also reported lower sleep quality, higher depression, anxiety and impulsivity. No association was found between AQoLS total score and socio-demographics, cognitive performance, or sleep quality in patients. We found a significant correlation between HRQoL and depression/anxiety as well as impulsivity. Anxiety and impulsivity were indeed the only significant predictors of HRQoL, explaining 47.7% of the variance. Conclusions. Anxiety and impulsivity are crucial determinants of HRQoL in recently detoxified sAUD patients. Since anxiety and impulsivity are frequent issues in addiction and especially in AUD, they should be particularly considered by clinicians to favour treatment outcomes.
Skin score as a predictor of prolonged QTc in systemic sclerosis.
Authors:   1.	Alireza Fatemi, Abdolahi Nafiseh , Aghaei Mehrdad ,   4.	Hamidreza Azimi, Mohammadi Ahmad 
Published: December 2021

Abstract

Background

Systemic sclerosis is an autoimmune disease characterized by endothelial dysfunction and fibrosis of the skin and internal organs. Cardiac involvement during systemic sclerosis can be primary or secondary to pulmonary arterial hypertension and renal pathology. Among the disorders in systemic sclerosis, prolongation of QTc time is also associated with more anti-RNA polymerase III antibodies, longer duration and severity of disease.
Methods

This case-control study was performed on 35 patients with systemic scleroderma who filled in the American Society of Rheumatism (ACR / EULAR criteria) and 35 healthy subjects prior to entering the study. Then, the QTc distance was extracted from the electrocardiogram and calculated using the formula. The measured QTc distance in the electrocardiogram, QTc> 440ms in men and QTc> 460ms in women, was defined as QTc long. Then the patients and the control group underwent echocardiography and changes in QTc interval and its relation with echocardiographic findings was evaluated.
Results

The results of this study indicated a significant relationship between QTc distance in patients with scleroderma compared with healthy controls. There was also a significant relationship between QTc and Skin Score of patients. However, there was no significant correlation between QTc distance and age, gender, duration of disease, Anti-Centromere, Anti-Scl70, and pulmonary artery pressure.
Conclusion

This study concludes that patients with scleroderma are at high risk for cardiac conduction impairment. The only factor that significantly correlated with QTc was the Skin Score of the patients.
Evaluation of treatment efficacy of tyrosine kinase inhibitors in rare single EGFR Exon 21 L861Q mutation; single center experience.
Authors: Gürsoy pınar 
Published: December 2021

Abstract
Aim:Epidermal growth factor receptor (EGFR) mutations are the second most common oncogenic driver event in non-small cell lung cancer (NSCLC).We aimed to compare the first generation erlotinib treatment with the second generation afatinib treatment in patients with Non-small Cell Lung Cancer (NSCLC) with Epidermal growth factor receptor (EGFR) Exon 21 L861Q mutation.Patients and Methods:Progression-free survival (PFS) and overall survival (OS) of 30 NSCLC patients treated with erlotinib or afatinib due to single EGFR L861Q positivity were compared retrospectively.Results:There were twenty-three patients in the erlotinib arm and seven patients in the afatinib arm. Median PFS was 12.8 months in the erlotinib group and 9.3 months in the afatinib group. Median overall survival in erlotinib and afatinib groups were 77.9 months and 30.3 months, respectively. No statistically significant difference was found in the comparison of these survival times.Conclusion:Survival times of erlotinib and afatinib treatment are similar in patients with a single EGFR L861Q mutation. In patients receiving tyrosine kinase inhibitors (TKI) treatment, female gender has a positive effect on PFS and being none-smoker has a positive effect on OS. In patients with rare mutation Exon 21 L861Q positivity both first-generation and second-generation TKIs should be considered.
­Comparison of Statistical Downscaling of Summer Daily Precipitation Through a Certain Perfect Prognosis and Bias Corrections - A Case Study Across China
Authors: Liu Yonghe , Wang Xiyue , Wang Mingshi , Wang Hailin 
Published: December 2021

Abstract
Fewer perfect prognosis (PP) based statistical downscaling were applied to future projections produced by global circulation models (GCM), when compared with the method of model output statistics (MOS). This study is a trial to use a multiple variable based PP downscaling for summer daily precipitation at many sites in China and to compare with the MOS. For the PP method (denoted as ‘OGB-PP’), predictors for each site are screened from surface-level variables in ERA-Interim reanalysis by an optimal grid-box method, then the biases in predictors are corrected and fitted to generalized linear models to downscale daily precipitation. The historical and the future simulations under the medium emission scenario (often represented as ‘RCP4.5’), produced by three GCMs (CanESM2, HadGEM2-ES and GFDL-ESM2G) in the coupled model intercomparison project phase five (CMIP5) were used as the downscaling bases. The bias correction based MOS downscaling (denoted as ‘BC-MOS’) were used to compare with the OGB-PP. The OGB-PP generally produced the climatological mean of summer precipitation across China, based on both ERAI and CMIP5 historical simulations. The downscaled spatial patterns of long-term changes are diverse, depending on the different GCMs, different predictor-bias corrections, and the choices on selecting PP and MOS. The annual variations downscaled by OGB-PP have small differences among the choices of different predictor-bias corrections, but have large difference to that downscaled by BC-MOS. The future changes downscaled from each GCM are sensitive to the bias corrections on predictors. The overall change patterns in some OGB-PP results on future projections produced similar trends as those projected by other multiple-model downscaling in CMIP5, while the result of the BC-MOS on the same GCMs did not, implying that PP methods may be promising. OGB-PP produced more significant increasing/decreasing trends and larger spatial variability of trends than the BC-MOS methods did. The reason maybe that in OGB-PP the independent precipitation modeling mechanism and the freely selected grid-box predictors can give rise to more diverse outputs over different sites than that from BC-MOS, which can contribute additional local variability.
Exposure to C. Albicans Makes Bladder Epithelial Cells Induce Apoptosis and Modification of Their Microenvironment
Authors: Kim Jayoung , Yeon Austin , Ahmed Khandakar Tanvir , Zhang Wei , Kim Khae-Hawn , Kim Woong-Ki 
Published: December 2021

Abstract
INTRODUCTION. Interstitial cystitis/painful bladder syndrome (IC) is characterized by chronic bladder pain and urinary storage symptoms. IC affects more than 3.3 million women in the U.S. alone. Ibis T-5000 assays and next generation sequencing have revealed that the C. albicans fungus is highly abundant in the urine of IC patients, particularly those who report greater pain, urinary urgency, and flares. However, currently, the clinical significance of C. albicans in the urine remains elusive. Here, we report the pathological effects and mechanisms triggered by C. albicans in a healthy normal bladder. METHODS. Immortalized bladder epithelial cells were infected with C. albicans. Perturbations in gene expression were identified using an Affymetrix gene microarray and subsequently followed with bioinformatic analyses, including gene set enrichment. Inflammatory and apoptotic genes were quantified using RT-PCR and Western blot analyses. Central signal pathways were examined using Western blot analysis. RESULTS. DNA microarray analysis showed alterations in the transcriptome of bladder epithelial cells infected with C. albicans over both the short and long terms. Key inflammatory and apoptosis networks were changed, which was consistent with several cellular events. Cellular levels of reactive oxygen species and nitrogen oxide increased after infection. Productions of cyclooxygenase-2 and prostaglandine E2 also increased after C. albicans infection, and their productions were suppressed by blockage of reactive oxygen species-epidermal growth factor receptor-Erk pathway. CONCLUSIONS. This study provides evidence that C. albicans infection triggers inflammation and cellular damage by dysregulating key regulatory genes, signaling pathways, and bioactive species in normal bladder cells.
Public toilets from the perspective of users: a case study in a public place, Brazil
Authors: Moreira Fernanda Deister , Rezende Sonaly , Passos Fabiana 
Published: December 2021

Abstract
Public toilets are essential infrastructure to guarantee the right to sanitation in public spaces and, in more general terms, the right to inclusive and sustainable cities. Moreover, since the equipment has a direct user interface, it is important to understand their demands and needs. Given this, the present research aims to understand the perspective of public toilet users on the Pampulha Lake Shore (PLS), a public touristic place in Belo Horizonte, Brazil. For that, observations and semi-structured interviews were carried out with different public toilet users. In addition, comments posted on the Google Local Guides tool of Google Maps were used as a secondary database for understanding users' experience of PLS toilets. The analysis made it possible to identify aspects related to availability, quality (health and hygiene), security, and accessibility. Collected data showed how the conflicting choice of whether or not to use the toilet was directly related to the health and conservation of the urban equipment and interfered with social and leisure prospects. On the whole, the importance of the user's perspective was highlighted in this study with emphasis on elaborating adequate urban planning with regard to health, sanitation, and accessibility issues.
A hora imortal da Universidade
Authors: Araújo Humberto Hermenegildo de 
Published: December 2021

Em“A hora imortal da Universidade” o acadêmico da Academia Norteriograndense de Letras Humberto Hermenegildo, "Ad immortalitem" faz uma análise do discurso de Câmara Cascudo intitulado Universidade e Civilização, durante a solenidade de instalação da UFRN. As relações desse discurso com os planos para a “Universidade popular” são traçadas, e a explicitação do que teria pensado Cascudo ao escrever aquele discurso é avaliada também em uma perspectiva atual. Ainda nesta sessão, o professor Willington Germano, pró-reitor de extensão no período 1999-2003 e professor emérito da UFRN, propõe a discussão sobre a “Educação em tempos de exceção”, uma avaliação dos retrocessos e ameaças “ao conhecimento crítico, aquele que pode auxiliar os indivíduos a fazerem escolhas e julgamento inerentes à atividade do pensamento”.