bluuebunny/crossref_metadata_2025_split huggingface dataset:
Androgenic Haploid Plant Development Via Embryogenesis With Simultaneous Determination of Bioactive Metabolites in Cambod Tea (Camellia Assamica ssp. Lasiocalyx)
Authors: Mishra Vijay Kumar , Bajpai Ruchira , Chaturvedi Rakhi 
Published: October 2021

Abstract
This pioneering work reports successful androgenic plant development via embryogenesis from microspore calluses in anther cultures and estimation of bioactive metabolites in in vitro regenerants and parent plant (control) of Cambod tea, Camellia assamica ssp. lasiocalyx (Planch MS) cultivar TV19. Anthers bearing microspores at early-to-late uni-nucleate stage were selected to initiate androgenesis. A pre-treatment of 50 C for five days in the dark was most effective to initiate profusely growing white callusing from microspores within 10 weeks of culture on MS medium (6% sucrose) supplemented with high cytokinins/ auxin ratio maintained by benzyl adenine (BAP) and 2,4-dichlorophenxyacetic acid (2, 4-D). Nodular structures on the callus surface differentiated into embryos. Further developement of the embryos occurred on embryogenesis medium but, with ten times reduced concentration of growth regulators and additives. Germination of embryos into complete plantlets was achieved when major salts in medium were reduced to half MS (½ MS) and augmented with BAP, GA3 and IBA along with glutamine and serine. Cytological examination of the root-tip cells revealed that regenerated plantlets were haploids (2n=x=15), which was further confirmed through flow cytometry. The hot-water extracts from in vitro haploid calluses, embryos and field-grown donor plant were utilized for quantification of (+)-catechin, (-)-epicatechin, (-)-epigallocatechin gallate, caffeine and theophylline. Our findings revealed that the metabolite profile of in vitro regenerated haploid cultures is comparable to that of the mother plant, thereby presenting them as potential source for genome duplication and development of genetically stable homozygous pure breeding lines.
Associations of Actigraphy-derived Rest-activity Patterns and Circadian Phase Parameters With Clinical Symptoms and Polysomnography Results in Patients With Chronic Insomnia
Authors: Roh Hyun Woong , Choi Su Jung , Jo Hyunjin , Kim Dongyeop , Choi Jung-gu , Son Sang Joon , Joo Eun Yeon 
Published: October 2021

Abstract
We explored the associations of actigraphy-derived rest-activity patterns and circadian phase parameters with clinical symptoms and level 1 polysomnography (PSG) results in patients with chronic insomnia to evaluate the clinical implications of actigraphy-derived parameters for PSG performance. Seventy-five participants underwent actigraphy assessments and level 1 PSG. Exploratory correlation analyses between parameters derived from actigraphy, PSG, and clinical assessments were performed. Study participants were classified into two groups, and group differences were investigated after adjusting for potential covariates. Participants with poorer rest-activity patterns on actigraphy (low inter-day stability and high intra-daily variability) exhibited higher insomnia severity index scores compared to participants with better rest-activity patterns. No between-group differences in PSG parameters were observed. Late-phase participants (late least active 5-h and most active 10-h onset times) exhibited higher insomnia severity scores, longer sleep latency, longer rapid eye movement latency, and lower apnea-hypopnea index compared to early-phase participants. These associations remained significant even after adjusting for potential covariates. Some actigraphy-derived rest-activity patterns and circadian phase parameters were significantly associated with clinical symptoms and PSG results, suggesting a possible adjunctive role of actigraphy-derived rest-activity patterns and circadian phase parameters in deriving plans for PSG lights-off time and assessing the possible insomnia pathophysiology.
Appropriateness of Referrals From Primary Care for Lumbar MRI
Authors: Krogh Susanne Brogaard , Jensen Tue Secher , Rolving Nanna , Laursen Malene , Thomsen Janus Nikolaj Laust , Hansen Casper Brink , Werenberg Christoffer Høj , Rasmussen Erik , Carlson Rune , Jensen Rikke Krüger 
Published: October 2021

Abstract
Background: A number of papers highlight the extent to which low back pain (LBP) is generally mismanaged, especially regarding overuse of magnetic resonance imaging (MRI). International guidelines do not recommend routine imaging, including MRI, and seek to guide clinicians only to refer for imaging based on specific indications. Despite this, several studies show an increase in the use of MRI among patients with LBP and an imbalance between appropriate versus inappropriate use of MRI for LBP. This study aimed to investigate to what extent referrals from general practice for lumbar MRI complied with clinical guideline recommendations in a Danish setting.Materials and methods: From 2014-2018, all referrals for lumbar MRI were included from general practitioners in the Central Denmark Region for diagnostic imaging at a public regional hospital. A modified version of the American College of Radiology Imaging Appropriateness Criteria for LBP was used to classify referrals as appropriate or inappropriate, based on the unstructured text in the GPs’ referrals. Appropriate referrals included fractures, cancer, symptoms persisting for more than 6 weeks of non-surgical treatment, previous surgery, candidate for surgery or suspicion of cauda equina. Inappropriate referrals were sub-classified as lacking information about previous non-surgical treatment and duration. Results: Of the 3,772 retrieved referrals for MRI of the lumbar spine, 55% were selected and a total of 2,051 referrals were categorised. Approximately one quarter (24.5%) were categorised as appropriate, and 75.5% were deemed inappropriate. 51% of the inappropriate referrals lacked information about previous non-surgical treatment, and 49% had no information about the duration of non-surgical treatment. Apart from minor yearly fluctuations, there was no change in the distribution of appropriate and inappropriate MRI referrals from 2014 to 2018.Conclusion:The majority of lumbar MRI referrals (75.5%) from general practitioners for lumbar MRI did not fulfil the ACR Imaging Appropriateness Criteria for LBP based on the unstructured text of their referrals. There is a need for referrers to include all guideline-relevant information in referrals for imaging. More research is needed to determine whether this is due to patients not fulfilling guideline recommendations or simply the content of the referrals.
Heterogenous Nanocomposite Catalysts With Rhenium Nanostructures for the Catalytic Reduction of 4-nitrophenol
Authors: Cyganowski Piotr , Dzimitrowicz Anna 
Published: October 2021

Abstract
Stable and efficient heterogenous nanocatalysts for the reduction of 4-nitrophenol (4-NP) has attracted much attention in recent years. In this context, a unique and efficient in-situ approach is used for the production of new polymeric nanocomposites (pNCs) containing rhenium nanostructures (ReNSs). These rare materials should facilitate the catalytic decomposition of 4-NP, in turn ensuring increased catalytic activity and stability. These nanomaterials were analyzed using Fourier-Transformation Infrared spectroscopy (FT-IR), transmission electron microscopy (TEM), and X-Ray powder diffraction (XRD). The efficiency of the catalytic reaction was estimated based on the acquired UV-Vis spectra, which enabled the estimation of the catalytic activity using pseud-first order modelling. The applied method resulted in the successful production and efficient loading of ReNSs in the polymeric matrices. Amino functionalities played a primary role in the reduction process. Moreover, the functionality that is derived from 1.1’-carbonyl imidazole improved the availability of the ReNSs, which resulted in 90% conversion of 4-NP with a maximum rate constant of 0.28 min-1 over 11 subsequent catalytic cycles. This effect was observed despite the trace amount of Re in the pNCs (~5%), suggesting a synergistic effect between the polymeric base and the ReNSs-based catalyst.
Apoptosis-Independent Reduction of Programmed Death Ligand-1 Levels by Boningmycin Through AMPK-Mediated Endoplasmic Reticulum-Associated Protein Degradation in Human Tumor Cells
Authors: Zhang Juan , Wang Jin-Cai , Shang Yue , Chen Yang , Chen Shu-Zhen , He Qi-Yang 
Published: October 2021

Abstract
Boningmycin (BON), a new member of the bleomycin family, exhibits highly potent activity against tumor cells in vitro and in vivo. It remains unclear if BON can affect the protein levels of programmed death ligand-1 (PD-L1) in a manner similar to that of other antitumor agents. Potent inhibition of cell survival by BON was observed in non-small-cell lung cancer NCI-H460 cells and sarcoma HT1080 cells. Apoptosis-independent reduction of PD-L1 was observed after exposure to BON. Furthermore, BON-treatment increased AMP-activated protein kinase phosphorylation, however, this increase was suppressed by treatment with specific inhibitor (compound C) or RNAi-mediated knockdown of AMPKα. BON-induced PD-L1 reduction is mediated by the endoplasmic reticulum-associated degradation pathway. Its mode of action is similar to that of metformin on the PD-L1 protein. In conclusion, it is firstly reported that BON can decrease PD-L1 protein levels through the AMPK activated endoplasmic reticulum- associated degradation pathway.
Modelling of pure CO2 and flue gas sorption data on South African coals using Langmuir, Freundlich, Temkin, and Extended Langmuir isotherm models
Authors: Mabuza Major , Premlall Kasturie , Daramola Michael O. 
Published: October 2021

Abstract
Greenhouse gases (GHGs) have sharply increased over the past four decades due to intensifying industrial activities; as a result, the earth has been faced with global warming in which the major contributor is the anthropogenic carbon dioxide (CO 2 ) emissions. Carbon sequestration in unmineable coal seams has been proposed as one of the most attractive technologies to mitigate CO 2 emissions in which CO 2 is stored in the microporous structure of the coal matrix in an adsorbed state. The CO 2 adsorption process is hence considered one of the more effective methodologies in environmental sciences. Thus, adsorption isotherm measurements and modelling are key important scientific measures required in understanding the adsorption system, mechanism, and process optimization in coalbeds. In this paper, three renowned adsorption isotherm models were employed including Langmuir, Freundlich, and Temkin for pure CO 2 adsorption data, and the Extended-Langmuir model for multicomponent, such as flue gas mixture-adsorption data as investigated in this research work. The adsorption data was acquired from a high-pressure volumetric sorption system (HPVSS) experiment involving two South African coal samples from Ermelo and Somkhele coalfields with pure CO 2 and synthetic industrial flue gas to simulate emissions that are representative of a typical coal-fired power plant (12% CO 2 , 5.5% O 2 , 82% N 2 , 0.38% SO 2 , and 0.12% NO 2 ). The adsorption data was measured on 10 g samples with a mean size of 2 mm at temperatures ranging from 30 ºC to 60 ºC and pressure up to 9.0 MPa using the HPVSS. The statistical evaluation of the goodness-of-fit was done using three (3) statistical data analysis methods including correlation coefficient (R 2 ), standard deviation ( σ ), and standard error (SE). The Langmuir isotherm model conventionally fits the pure CO 2 gas experimental data better than Freundlich and Temkin. The Extended Langmuir gives best experimental data fit for the flue gas.
On the Feasibility of All-solid-state Batteries with LLZO as a Single Electrolyte
Authors: Kravchyk Kostiantyn V. , Karabay Dogan Tarik , Kovalenko Maksym V. 
Published: October 2021

Abstract
Replacement of Li-ion liquid-state electrolytes by solid-state counterparts in a Li-ion battery (LIB) is a major research objective as well as an urgent priority for the industry, as it enables the use of a Li metal anode and provides new opportunities to realize safe, non-flammable, and temperature-resilient batteries. Among the plethora of solid-state electrolytes (SSEs) investigated, garnet-type Li-ion electrolytes based on cubic Li7La3Zr2O12 (LLZO) are considered the most appealing candidates for the development of future solid-state batteries because of their low electronic conductivity of ca. 10−8 S cm−1 (RT) and a wide electrochemical operation window of 0 ‒ 6 V vs. Li+/Li. However, high LLZO density (5.1 g cm-3) and its lower level of Li-ion conductivity (up to 1 mS cm−1 at RT) compared to liquid electrolytes (1.28 g cm-3; ca. 10 mS cm−1 at RT) still raise the question as to the feasibility of using solely LLZO as an electrolyte for achieving competitive energy and power densities. In this work, we analyzed the energy densities of Li-garnet all-solid-state batteries based solely on LLZO SSE by modeling their Ragone plots using LiCoO2 as the model cathode material. This assessment allowed us to identify values of the LLZO thickness, cathode areal capacity, and LLZO content in the solid-state cathode required to match the energy density of conventional lithium-ion batteries (ca. 180 Wh kg-1 and 497 Wh L-1) at the power densities of 200 W kg-1 and 600 W L-1, corresponding to ca. 1h of battery discharge time (1C). We then discuss key challenges in the practical deployment of LLZO SSE in the fabrication of Li-garnet all-solid-state batteries.
Relationship Between Pathological Response and Molecular Subtypes in Locally Advanced Breast Cancer Patients Receiving Neoadjuvant Chemotherapy
Authors: DEĞERLİ EZGİ , Öztaş Nihan Şentürk , Alkan Gülin , Bedir Şahin , Derin Sümeyra , Valıkhanova Nahıda , Saraç Betül , Demirci Nebi Serkan , Demirelli Hulusi Fuat , Turna Hande 
Published: October 2021

Abstract
Background: More than half of patients with breast cancer were diagnosed with locally advanced stages of the disease (54%). This study aimed to explain the pathological response received with neoadjuvant chemotherapy (NACT) according to the molecular classification of breast cancer in patients with locally advanced tumors.Methods: One hundred one patients with locally advanced breast cancer treated with neoadjuvant chemotherapy were analyzed. Patients were classified into five molecular subtypes based on the profile of the estrogen receptor, progesterone receptor, HER2, and Ki-67. We determined associations between pathologic complete response and molecular subgroups.Results: Most patients had luminal A tumors (n:28, 27.7%). The overall rate of pathological complete response (pCR) was 34.7% (n:35). Tumors that presented the highest rate of pCR were pure HER2-positive, at 60% (n:6; OR, 3.2; 95% CI, 0.8–12.2). According to logistic regression analysis, the factors affecting pCR were HER2 positivity and clinically positive axilla before NACT. Luminal A tumors had a significantly lower pCR rate. (7.1%,p: 0.001). Despite the low pCR rate, luminal A tumor survival was the best subgroup (p< 0.001). However, there was no difference between EFS and OS according to pCR in any molecular subgroups.Conclusion: Pathological complete response is directly related to the subtypes of breast cancer. A high rate of complete pathological response is observed in the pure HER2-positive group. However, EFS and OS were not statistically significant in patients with and without pCR.
Magnetic Activated Cell-Sorting Identifies a Unique Lung Microbiome Community Associated with Disease States
Authors: Dunlap Daniel , Yang Libing , Qin Shulin , Li Kelvin , Fitch Adam , Huang Laurence , McVerry Bryan J , Methé Barbara A , Morris Alison 
Published: October 2021

Abstract
Background: The advent of culture-independent, next-generation DNA sequencing has led to discovery of distinct lung bacterial communities. Studies of lung microbiome taxonomy often reveal only subtle differences between health and disease, but microbial host response may distinguish members of similar communities in different populations. Magnetic-activated cell sorting has been applied to the gut microbiome to identify numbers and types of bacteria eliciting a humoral response. We adapted this technique to examine populations of immunoglobulin-bound bacteria and investigate the lung microbiota in Human Immunodeficiency Virus (HIV) as a representative disease.Methods: 42 people living with HIV and 22 HIV-uninfected individuals underwent bronchoalveolar lavage. We separated immunoglobulin G-bound bacteria using magnetic-activated cell sorting and sequenced the 16S rRNA gene on the Illumina MiSeq platform. We analyzed microbial sequencing data and quantified cytokines and bacterial rRNA copy numbers from bronchoalveolar lavage. Results: Immunoglobulin G-bound bacteria were detectable in the healthy lung microbiota. Comparison of raw bronchoalveolar lavage by HIV status showed no significant taxonomic differences, but the immunoglobulin-bound lung microbiota differed by HIV status with greater abundance of Pseudomonas in people living with HIV. Lung cytokine levels were also higher in people living with HIV, which correlated with increased quantity of immunoglobulin G-bound bacteria. Conclusions: We report a novel application of magnetic-activated cell sorting to identify immunoglobulin G-bound bacteria in the lung. This technique identified distinct bacterial communities which differed in composition from raw bronchoalveolar lavage, revealing differences in health and disease not detected by traditional analyses. Cytokine response was also associated with differential immunoglobulin binding of lung bacteria, suggesting functional importance of these communities.
Secondary Consolidation Characteristics of Organic Soil Modified by Biological Enzymes Based on Gibson Model
Authors: Zhang Yixin , Wen Changping 
Published: October 2021

Abstract
In order to study the secondary consolidation characteristics of organic soil modified by enzyme, the secondary consolidation tests of organic soil modified by enzyme were carried out. Firstly, the consolidation coefficient Cv, secondary consolidation coefficient Ca and compression index Cc of modified organic soil under different levels of loads and different enzyme contents were obtained according to the analysis of experimental data. Then, the parameters of Gibson rheological model were fitted respectively according to the experimental results. Finally, the relationship between rheological model parameters, Ca/Cc, secondary consolidation coefficient Ca and loading was analyzed under different enzyme contents. The results show that: (1) The rheological model parameters of organic soil modified by enzyme are negatively correlated with the enzyme content, and positively correlated with the load value. When the enzyme content is 0.01%, some parameters reach the maximum value; (2) The secondary consolidation coefficient is related to the load. The secondary consolidation coefficient of samples with different enzyme contents shows a certain rule under all levels of load. With the increasing of load, the final secondary consolidation coefficient Ca approaches a stable value; (3) The Ca/Cc values of the samples of the modified organic soil with different enzyme contents are between 0.042 and 0.1 under different loads. The results show that the model is suitable for describing the secondary consolidation creep characteristics of organic soil, and can be used to guide the long-term deformation prediction of organic soil foundation.
The Effectiveness of integrating spirituality into prenatal care on Improving Sleep Quality and Insomnia Severity among Pregnant Women: A Randomized Control Trial
Authors: maleki azam , Moradi Nahid , Zenoozian Saeedeh 
Published: October 2021

Abstract
Background

To determine the effectiveness of integrating spirituality into prenatal care on Improving Sleep Quality and Insomnia Severity among Pregnant Women.

Methods

This randomized controlled trial was carried out on 40 pregnant women recruited at five health centers of zanjan, Iran, 2020. The eligible women were allocated into two intervention and control groups according to the randomized design. Group counselling with spiritual content was carried out in eight sessions, two times a week at 16 to 20 weeks of gestation. The control group only received routine care. Data were collected using the Pittsburgh Sleep Quality Index (PSQI) and the Insomnia Severity Index (ISI) questionnaires in three stages, before the intervention, at 28 and 36 weeks of gestation. Statistical analysis was performed using the SPSS 16.0 software (SPSS Inc., Chicago, IL, USA).

Results

The results showed that comparing the mean score of insomnia severity, sleep quality and its components in the pre-intervention phase was not statistically significant between the two groups(p>0.05). Comparison of the insomnia severity, sleep quality, and its components scores decreased statistically in the second and third trimesters compared to the first trimester in the intervention group than the control group(p<0.05). The effect of the intervention (Eta score) was 64%.

Conclusion

The results showed that counselling with spiritual content could effectively ameliorate sleep quality and reduce insomnia severity in pregnant women. It seems that the approach is an acceptable basis to design intervention programs in this field that can be considered by midwives.
Economic Growth and Quality Adjusted Human Capital Equation: Moderating Role of Social Capabilities in Africa
Authors: Joseph Tonuchi Emmanuel , Obikaonu Pauline Chimuru 
Published: October 2021

Abstract
The role of human capital on economic growth across countries has over time garnered lots of discussion in economic literature. This is fundamental, given that the actual determinant of the difference in income per capita across countries or why some countries are growing faster than other countries has remained an unresolved issue. This study provide a different insight into the nexus between human capital and economic growth by accounting for the role of social capabilities in a panel framework. Specifically, the study covers 40 African countries between 1998-2019, where General Method of Moment (GMM) was employed to estimate the model. Specifically, it was discovered that without improved legal institution and better economic opportunities, human capital impact on the growth of income per capita across countries is insignificant though positive. The study concludes that the effectiveness of knowledge accumulation and adoption of technology in a country is hinged on the availability of enhanced legal, social, and economic environment.JEL CODE: A3, D83, E24, F63, I2