bluuebunny/crossref_metadata_2025_split huggingface dataset:
Compartment-specific metabolome labeling enables the identification of subcellular fluxes that may serve as promising metabolic engineering targets in CHO cells
Authors: Wijaya Andy Wiranata , Ulmer Andreas , Hundsdorfer Lara , Verhagen Natascha , Teleki Attila , Takors Ralf 
Published: September 2021

Abstract13C labeling data are used to calculate quantitative intracellular flux patterns reflecting in vivo conditions. Given that approaches for compartment-specific metabolomics exist, the benefits they offer compared to conventional non-compartmented 13C flux studies remain to be determined. Using compartment-specific labeling information of IgG1-producing Chinese hamster ovary cells, this study investigated differences of flux patterns exploiting and ignoring metabolic labeling data of cytosol and mitochondria. Although cellular analysis provided good estimates for the majority of intracellular fluxes, half of the mitochondrial transporters, and NADH and ATP balances, severe differences were found for some reactions. Accurate flux estimations of almost all iso-enzymes heavily depended on the sub-cellular labeling information. Furthermore, key discrepancies were found for the mitochondrial carriers vAGC1 (Aspartate/Glutamate antiporter), vDIC (Malate/H+ symporter), and vOGC (α-ketoglutarate/malate antiporter). Special emphasis is given to the flux of cytosolic malic enzyme (vME): it could not be estimated without the compartment-specific malate labeling information. Interesting enough, cytosolic malic enzyme is an important metabolic engineering target for improving cell-specific IgG1 productivity. Hence, compartment-specific 13C labeling analysis serves as prerequisite for related metabolic engineering studies.
ABSTAT-HD: a scalable tool for profiling very large knowledge graphs
Authors: Alva Principe Renzo Arturo , Maurino Andrea , Palmonari Matteo , Ciavotta Michele , Spahiu Blerina 
Published: September 2021

AbstractProcessing large-scale and highly interconnected Knowledge Graphs (KG) is becoming crucial for many applications such as recommender systems, question answering, etc. Profiling approaches have been proposed to summarize large KGs with the aim to produce concise and meaningful representation so that they can be easily managed. However, constructing profiles and calculating several statistics such as cardinality descriptors or inferences are resource expensive. In this paper, we present ABSTAT-HD, a highly distributed profiling tool that supports users in profiling and understanding big and complex knowledge graphs. We demonstrate the impact of the new architecture of ABSTAT-HD by presenting a set of experiments that show its scalability with respect to three dimensions of the data to be processed: size, complexity and workload. The experimentation shows that our profiling framework provides informative and concise profiles, and can process and manage very large KGs.
Fieberkrämpfe – Diagnostik und Behandlung
Authors: Feucht Martha , Glatter Sarah , Pimpel Birgit 
Published: September 2021

ZusammenfassungObwohl nicht unmittelbar den Epilepsien zuzuordnen, gehören Fieberkrämpfe (FK) zu den häufigsten Anfallserkrankungen des Kindesalters (Lebenszeitprävalenz: 2–5 %). Entsprechend Definitionsvorschlag der Internationalen Liga gegen Epilepsie (ILAE) sind FK epileptische Anfälle, die im Rahmen fieberhafter Infekte (Temperatur > 38 °C) bei neurologisch gesunden Kindern zwischen 6 und 60 Monaten auftreten. Drei Subkategorien sind beschrieben: einfacher FK (70–90 % aller FK), komplizierter FK (10–35 % aller FK) und febriler Status epilepticus (FSE). Die Pathophysiologie von FK ist unklar, aktuell wird eine multifaktorielle – (poly)genetische und durch Umweltfaktoren bedingte – Genese favorisiert. Die Langzeitprognose ist günstig (Spontanremission in 95 %). Rezente Studien berichten jedoch über gehäuft auftretende neuropsychiatrische Störungen. Das Risiko, nach FK eine Epilepsie zu entwickeln, variiert je nach FK-Typ, ist aber generell nicht wesentlich höher als jenes in der Allgemeinbevölkerung. Folgende, mit FK assoziierte Syndrome weisen ein erhöhtes Risiko für eine nachfolgende Epilepsie bzw. irreversible neurologische Folgeschäden auf: die fieberinduzierte refraktäre epileptische Enzephalopathie von Schulkindern (FIRES), das Hemikonvulsions-Hemiplegie-Epilepsie(HHE)-Syndrom und die genetische/generalisierte Epilepsie mit Fieberkrämpfen plus (GEFS+). Die kausale Rolle komplizierter FK oder FSE für nachfolgende Ammonshornsklerose (AHS) und pharmakoresistente mesiale Temporallappenepilepsie (mTLE) ist aktuellen experimentellen und klinischen Daten zufolge nicht bewiesen, eine primäre Vorschädigung des Hippocampus als wahrscheinlicher anzunehmen. Das folgende Update beinhaltet – basierend auf aktueller Evidenz – Empfehlungen für (Differenzial‑)Diagnostik und Management von FK.
Myrmecovory in Neotropical primates
Authors: Ferreira Nadja I. Risch , Verhaagh Manfred , Heymann Eckhard W. 
Published: September 2021

AbstractAnts are the dominant group of animals in many habitats, particularly in tropical rainforests. High abundance and formation of large colonies convert them into a potential food source for a broad spectrum of animals. In this paper we review myrmecovory (consumption of ants) in Neotropical primates. Myrmecovory has been reported from 57 taxa (species + subspecies) out of 217 species of Neotropical primates, representing 18 out of 22 genera. The proportion of ants in the animal portion of the diet is highest amongst members of the genera Cebus, Sapajus, Cheracebus and Plecturocebus, but generally low in callitrichids, large pitheciids (Cacajao, Chiropotes) and atelids. Ants from seven subfamilies of Formicidae (out of 13 subfamilies found in the Neotropics) are consumed, including taxa with and without functional sting and with varying other defences. Foraging technics employed in myrmecovory range from picking ants from open substrates to extractive foraging involving the destruction of ant nests or shelters, but tool use has not been reported. We conclude that myrmecovory is widespread amongst Neotropical primates but on average contributes only a minor proportion of the diet. The diversity of foraging technics employed and lack of tool use in Neotropical primate myrmecovory, even for ants with functional stings and aggressive biting, suggests that tool use for myrmecovory in hominids has not evolved in response to ant defences but is a consequence of enhanced cognitive skills that evolved under other selection pressures.
Numerical solution to the Falkner-Skan equation: a novel numerical approach through the new rational a-polynomials
Authors: Abbasbandy S. , Hajishafieiha J. 
Published: September 2021

AbstractThe new rational a-polynomials are used to solve the Falkner-Skan equation. These polynomials are equipped with an auxiliary parameter. The approximated solution to the Falkner-Skan equation is obtained by the new rational a-polynomials with unknown coefficients. To find the unknown coefficients and the auxiliary parameter contained in the polynomials, the collocation method with Chebyshev-Gauss points is used. The numerical examples show the efficiency of this method.
Unrestricted gene flow between two subspecies of translocated brushtail possums (Trichosurus vulpecula) in Aotearoa New Zealand
Authors: Pattabiraman Nimeshika , Morgan-Richards Mary , Powlesland Ralph , Trewick Steven A. 
Published: September 2021

AbstractTwo lineages of brushtail possums (Trichosurus vulpecula) were historically introduced to Aotearoa New Zealand, and these two subspecies have different phenotypic forms. Despite over 100 years of potential interbreeding, they appear to retain morphological differences, which may indicate reproductive isolation. We examined this using population samples from a confined landscape and scored each specimen for phenotype using a number of fur colour traits. This resulted in a bimodal trait distribution expected for segregated grey and black lineages. We also sought evidence for genetic partitioning based on spatial and temporal effects. Genetic structure and rates of genetic mixing were determined using seven neutral, species-specific nuclear microsatellite markers and mitochondrial DNA control region sequence. Genotype analyses indicated high levels of variation and mtDNA sequences formed two major haplogroups. Pairwise tests for population differentiation of these markers found no evidence of subdivision, indicating that these brushtail possums behave as a single randomly mating unit. Despite maintenance of two main colour phenotypes with relatively few intermediates, previous inference of assortative mating and anecdotes of distinct races, our data indicate that New Zealand brushtail possums can freely interbreed, and that in some locations they have formed completely mixed populations where neutral genetic markers are unrelated to phenotype. This has implications for effective pest management towards eradication.
Consistency of thresholds for eutrophication assessments, examples and recommendations
Authors: Topcu D. , Brockmann U. 
Published: September 2021

AbstractInternational harmonisation of management goals for eutrophication processes in coastal waters, requiring reduction of discharges and depositions of nutrients and organic matter, needs coordinated assessments and measures. This is especially necessary in open areas, connected by currents and mixing processes with trans-boundary exchanges. Management goals, defined nationally as local thresholds for nutrients and chlorophyll-a, had been applied recently (2006–2014) within international eutrophication assessments in the North Sea (OSPAR) and Baltic Sea (HELCOM). Consistency of thresholds for nitrogen nutrients and chlorophyll-a concentrations is tested by mixing diagrams and correlations between nitrogen nutrients (total and inorganic nitrogen) and chlorophyll-a. Results indicate mean consistent relations, but single deviations as in the continental coastal water of the North Sea surpassed means by a factor up to 5 for chlorophyll-a in relation to inorganic nitrogen. Thresholds differed across national borders significantly. Correlations of thresholds and assed data reflect the degree of regional deviations by comparison. Consistency of regionally applied thresholds can be achieved stepwise, by application of regionally correlated means, by adaptation to mixing and parameter relations, and finally by relations of thresholds to natural background concentrations. By this, consistency of international assessments can be improved generally, allowing coordinated management of open coastal waters.
How are project-specific forums utilized? A study of participation, content, and sentiment in the Eclipse ecosystem
Authors: Nugroho Yusuf Sulistyo , Islam Syful , Nakasai Keitaro , Rehman Ifraz , Hata Hideaki , Kula Raula Gaikovina , Nagappan Meiyappan , Matsumoto Kenichi 
Published: September 2021

AbstractAlthough many software development projects have moved their developer discussion forums to generic platforms such as Stack Overflow, Eclipse has been steadfast in hosting their self-supported community forums. While recent studies show forums share similarities to generic communication channels, it is unknown how project-specific forums are utilized. In this paper, we analyze 832,058 forum threads and their linkages to four systems with 2,170 connected contributors to understand the participation, content and sentiment. Results show that Seniors are the most active participants to respond bug and non-bug-related threads in the forums (i.e., 66.1% and 45.5%), and sentiment among developers are inconsistent while knowledge sharing within Eclipse. We recommend the users to identify appropriate topics and ask in a positive procedural way when joining forums. For developers, preparing project-specific forums could be an option to bridge the communication between members. Irrespective of the popularity of Stack Overflow, we argue the benefits of using project-specific forum initiatives, such as GitHub Discussions, are needed to cultivate a community and its ecosystem.
A systemic approach to tackling ocean plastic debris
Authors: De Matteis Alessandro , Turkmen Ceylan Fethiye Burcu , Daoud Mona , Kahuthu Anne 
Published: September 2021

AbstractGrowing evidence of the prevalence of plastics in the oceanic environment is causing increasing concern. In this study we consider the possible impact of major changes to the supply and consumption of plastics. While we recognize that tackling the problem of plastic debris in the ocean calls for integrated solutions, our analysis contributes to filling the gap in knowledge about the effectiveness of intervention strategies. Our results find that strategies addressing plastics demand have a greater impact than those focussing on plastics supply. However, only measures aimed at plastics supply are effective in the short run. These considerations can help to inspire the prioritization of intervention strategies and to contribute to the design of integrated interventions aimed at tackling the problem of marine plastic debris through a systemic approach.
Engaging Transformation: Using Seasonal Rounds to Anticipate Climate Change
Authors: Kassam Karim-Aly , Ruelle Morgan , Haag Isabell , Bulbulshoev Umed , Kaziev Daler , Louis Leo , Ullmann Anna , Edwards Iriel , Khan Aziz Ali , Trabucco Antonio , Samimi Cyrus 
Published: September 2021

AbstractSeasonal rounds are deliberative articulations of a community’s sociocultural relations with their ecological system. The process of visualizing seasonal rounds informs transdisciplinary research. We present a methodological approach forcommunities of enquiryto engagecommunities of practicethrough context-specific sociocultural and ecological relations driven by seasonal change. We first discuss historical précis of the concept of seasonal rounds that we apply to assess the spatial and temporal communal migrations and then describe current international research among Indigenous and rural communities in North America and Central Asia by the creation of a common vocabulary through mutual respect for multiple ways of knowing, validation of co-generated knowledge, and insights into seasonal change. By investigating the relationship between specific biophysical indicators and livelihoods of local communities, we demonstrate that seasonal rounds are an inclusive and participatory methodology that brings together diverse Indigenous and rural voices to anticipate anthropogenic climate change.
Mean-field theory of the interaction of the magnesium ion with biopolymers: the case of lysozyme
Authors: Odijk Theo 
Published: September 2021

AbstractA statistical theory is presented of the magnesium ion interacting with lysozyme under conditions where the latter is positively charged. Temporarily assuming magnesium is not noncovalently bound to the protein, I solve the nonlinear Poisson–Boltzmann equation accurately and uniformly in a perturbative fashion. The resulting expression for the effective charge, which is larger than nominal owing to overshooting, is subtle and cannot be asymptotically expanded at high ionic strengths that are practical. An adhesive potential taken from earlier work together with the assumption of possibly bound magnesium is then fitted to be in accord with measurements of the second virial coefficient by Tessier et al. The resulting numbers of bound magnesium ions as a function of MgBr$$_2$$

2

concentration are entirely reasonable compared with densitometry measurements.
Relative educational poverty: conceptual and empirical issues
Authors: Glaesser Judith 
Published: September 2021

AbstractThis paper’s goal is to discuss implications for the empirical study of low educational status arising from the use of the concept of educational poverty in research. It has two related conceptual foci: (1) the relationship of educational poverty with material poverty and to what extent useful parallels exist, and (2) the distinction of absolute and relative (educational) poverty and whether the notion of absolute (educational) poverty is a sensible one. For the concept of educational poverty to be analytically fruitful, clear conceptualisation and operationalisation of the relevant issues are required. The paper contributes to the aim of providing these by building on existing work on educational poverty and by drawing on relevant work on material poverty as well as discussing some conceptual challenges and some of the challenges arising from the operationalisation of the concepts. Some of these challenges are illustrated using examples based on data from the German National Educational Panel Study (NEPS). In a further step, factors which may lead to a greater risk of being in relative educational poverty are analysed, employing the method multi-value Qualitative Comparative Analysis. The empirical findings highlight the relative nature of educational qualifications: the usefulness of a basic school leaving qualification has changed over time, and it has not been the same for different groups. Thus, a conceptualisation of low educational status as educational poverty has been shown to be useful, and it has been demonstrated that the relative nature of educational poverty ought to be taken into account by researchers.
Detecting and Characterising Small-Scale Brightenings in Solar Imaging Data
Authors: Humphries Llŷr Dafydd , Morgan Huw , Kuridze David 
Published: September 2021

AbstractObservations of small-scale brightenings in the low solar atmosphere can provide valuable constraints on possible heating and heat transport mechanisms. We present a method for the detection and analysis of brightenings, and demonstrate its application to time-series imagery of the Interface Region Imaging Spectrograph (IRIS) in the extreme ultraviolet (EUV). The method is based on spatio-temporal band-pass filtering, adaptive thresholding and centroid tracking, and records an event’s spatial position, duration, total brightness and maximum brightness. Spatial area, brightness, and position are also recorded as functions of time throughout the event’s lifetime. Detected brightenings can fragment, or merge, over time – thus the number of distinct regions constituting a brightening event is recorded over time, and the maximum number of regions recorded as $N_{\mathit{frag}}$

N
frag

, which is a simple measure of an event’s coherence or spatial complexity. A test is made on a synthetic datacube composed of a static background based on IRIS data, Poisson noise and $\approx 10^{4}$
≈

10

4

randomly-distributed, moving, small-scale Gaussian brightenings. Maximum brightness, total brightness, area, and duration follow power-law distributions, and the results show the range over which the method can successfully extract information. The test shows that the recorded maximum brightness of an event is a reliable measure for the brightest and most accurately detected events, with an error of 6%. Event area, duration and speed are generally underestimated by around 15% and have an uncertainty of 20–30%. The total brightness is underestimated by 30%, and has an uncertainty of 30%. Applying this detection method to real IRIS quiet-sun data spanning 19 minutes over a $54.40''\times 55.23''$

54.40
″

×

55.23
″

field of view (FOV) yields 2997 detections, 1340 of these detections either remain un-fragmented or fragment to two distinct regions at least once during their lifetime ($N_{\mathit{frag}}\le 2$

N
frag

≤
2
), equating to an event density of $3.96\times 10^{-4}$
3.96
×

10

−
4

arcsec−2 s−1. The method will be used for a future large-scale statistical analysis of several quiet-sun (QS) data sets from IRIS, other EUV imagers, and other types of data including H$\alpha $
α
and visible photospheric imagery.
Parents’ Attachment Styles and Adolescents’ Regulatory Emotional Self-efficacy: The Mediating Role of Adolescents’ Attachment to Parents in China
Authors: Pan Yangu , Zhang Qiongwen , Liu Guangzeng , Li Bingbing , Liu Chuanxing 
Published: September 2021

AbstractGuided by attachment theory, this longitudinal study examined the mediating role of parent-adolescent attachment on the relation between parents’ attachment styles and adolescents’ regulatory emotional self-efficacy (RESE, including managing negative affect and expressing positive affect). Five hundred seventy-three Chinese junior high school students (46% male; aged 11–14 years, M = 12.76 years, SD = 0.74) completed measures of RESE at T1, parent-adolescent attachment at T2 (six months later), and RESE at T3 (another six months later), while 573 students’ parents (one student only has a parent, 241 fathers and 332 mothers) completed measures of adult attachment styles (anxiety and avoidance) at T1. Results from structural equation modeling indicated that father-adolescent attachment mediated the association between fathers’ attachment anxiety and adolescents’ self-efficacy beliefs in managing negative affect, while mother-adolescent attachment marginally mediated the relation between mothers’ attachment anxiety and adolescents’ self-efficacy beliefs in managing negative affect and expressing positive affect. These findings suggest that parents’ attachment anxiety could predict their children’s attachment to parents, in turn, impacting their children’s regulatory emotional self-efficacy.
Addressing Learner-Centred Barriers to Sexuality Education in Rural Areas of South Africa: Learners’ Perspectives on Promoting Sexual Health Outcomes
Authors: Adekola Ayobami Precious , Mavhandu-Mudzusi Azwihangwisi Helen 
Published: September 2021

Abstract
Introduction
The school-based sexuality education programmes in South Africa aim to improve the sexual and reproductive health of school-going adolescents. However, the high rate of unplanned pregnancy and sexually transmitted infections among learners in some schools in rural areas of King Cetshwayo district suggests that the programmes in these schools might not be effective due to certain learner-centred factors.

Method
This qualitative study explored lived experiences of 84 learners from nine public schools in 2020 through focus group interviews. Data was analysed using Interpretative Phenomenological Analysis.

Results
Learner-centred barriers to effective school-based sexuality education identified in this study were attitudes, age disparity, psychological status, peer pressure, socio-economic status, the exploratory attitude of learners, media, lack of role models, previous experiences, socio-economic status, and lack of parental love. These factors could reduce good sexual health. Learner-targeted interventions such as campaigns, using guest professionals, condom distribution, videos, on-site family planning, formal demonstrations, and on-site counselling could address these barriers.

Conclusions
Addressing these barriers and implementing the proposed interventions will enhance school-based sexuality education and consequently improve adolescents’ sexual health.

Policy Implications
The findings could guide programming, implementation, and delivery of school-based sexuality education leading to improved adolescents’ sexual and reproductive health.
Therapeutic cell engineering: designing programmable synthetic genetic circuits in mammalian cells
Authors: Mansouri Maysam , Fussenegger Martin 
Published: September 2021

AbstractCell therapy approaches that employ engineered mammalian cells for on-demand production of therapeutic agents in the patient’s body are moving beyond proof-of-concept in translational medicine. The therapeutic cells can be customized to sense user-defined signals, process them, and respond in a programmable and predictable way. In this paper, we introduce the available tools and strategies employed to design therapeutic cells. Then, various approaches to control cell behaviors, including open-loop and closed-loop systems, are discussed. We also highlight therapeutic applications of engineered cells for early diagnosis and treatment of various diseases in the clinic and in experimental disease models. Finally, we consider emerging technologies such as digital devices and their potential for incorporation into future cell-based therapies.