bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Life Cycle Assessment of Guar Agriculture
Authors: Mealing VeeAnder S. , Landis Amy E. 
Published: July 2021

Abstract
Guar gum, the main product of the guar crop, is used widely in the US as an emulsifier in the food industry and as fracturing fluid additive in the oil and gas industry. The US is the number one global importer of guar, and interest has grown to domestically cultivate guar in the US. Guar is an annual desert legume native to India and Pakistan. The goal of this study was to evaluate the environmental sustainability of growing guar in the U.S. via a life cycle analysis (LCA). The LCA helps identify the information gap for US agriculture and guide future field studies to optimize guar cultivation in the US. This study concluded that in terms of environmental sustainability, irrigation, harvesting, and P-fertilization methods offer the most opportunity for improved guar agricultural sustainability. This is promising because one of guar’s prominent characteristics is its high water use efficiency and ability to grow in marginal soils. Lowering irrigation and water use can be implemented with simple management practice changes like optimizing irrigation. In addition, this study shows that there is an opportunity for field trials to optimize fertilizer application rates to achieve the greatest yields. This study also found a knowledge gap with respect to C soil fluxes and field emissions of N and P from guar agriculture. As the United States pursues adopting guar agriculture in the Southwest, it will be critical to evaluate irrigation to achieve maximum yields (e.g. drip, flood, sprinkler) and fill fertilizer and emissions knowledge gaps.
The intra-mitochondrial O-GlcNAcylation system acutely regulates OXPHOS capacity and ROS dynamics in the heart
Authors: Dontaine Justine , Bouali Asma , Daussin Frederic , Bultot Laurent , Vertommen Didier , Martin Manon , Rathagirishnan Rahulan , Cuillerier Alexanne , Horman Sandrine , Beauloye Christophe , Gatto Laurent , Lauzier Benjamin , Bertrand Luc , Burelle Yan 
Published: July 2021

Abstract
Protein O-GlcNAcylation is increasingly recognized as an important cellular regulatory
mechanism, in multiple organs including the heart. However, the mechanisms leading to O-GlcNAcylation in mitochondria and the consequences on their function remain poorly understood. In this study, we used an in vitro reconstitution assay to characterize the intra-mitochondrial O- GlcNAc system without potential cytoplasmic confounding effects. We compared the O-GlcNAcylome of isolated cardiac mitochondria with that of mitochondria acutely exposed to NButGT, a specific O-GlcNAcylation inducer. Amongst the 409 O-GlcNAcylated mitochondrial proteins identified, 191 displayed increased O-GlcNAcylation in response to NButGT. This was associated with enhanced Complex I (CI) activity, increased maximal respiration in presence of CI substrates, and a striking reduction of mitochondrial ROS release, which could be related to O- GlcNAcylation of subunits within the NADH dehydrogenase module of CI. In conclusion, our
work underlines the existence of a dynamic mitochondrial O-GlcNAcylation system capable of rapidly modifying mitochondrial function.
Dynamics of a Guanaco-sheep Competitive System with Unilateral and Bilateral Control
Authors: Xu Jing , Huang Mingzhan , Song Xinyu 
Published: July 2021

Abstract
In this paper, based on a guanaco-sheep competitive system, we develop and analyze mathematical models with unilateral and bilateral control for the management of overgrazing. We first analyze the dynamics of the uncontrolled system. Then, we investigate the system with impulsive control by differential equation geometry theory. And we mainly prove the existence and stability of order-1 periodic solution for unilateral control system and order-2 periodic solution for bilateral control system. Comparing the unilateral and bilateral control strategy, we encourage bilateral control rather than unilateral control for the management of sheep species.
A Time-Driven Cloudlet Placement Strategy for Workflow Applications in Wireless Metropolitan Area Networks
Authors: zhang Jianshan , Li Ming , Zheng Xianghan , Hsu Ching-Hsien 
Published: July 2021

Abstract

With the rapid development of mobile technology, mobile applications have increasing requirements for computational resources, and mobile devices can no longer meet these requirements. Mobile edge computing (MEC) has emerged in this context and has brought innovation into the working mode of traditional cloud computing. By provisioning edge server placement, the computing power of the cloud centre is distributed to the edge of the network. The abundant computational resources of edge servers compensate for the lack of mobile devices and shorten the communication delay between servers and users. Constituting a specific forms of edge servers, cloudlets have been widely studied within academia and industry in recent years. However, existing studies have mainly focused on computation offloading for general computing tasks under fixed cloudlet placement positions. They ignored the impact on computation offloading results from cloudlet placement positions and data dependencies among mobile application components. In this paper, we study the cloudlet placement problem based on Workflow Applications (WAs) in Wireless Metropolitan Area Networks (WMANs). We devise a cloudlet placement strategy based on a Particle swarm optimization algorithm using Genetic algorithm operators with the Encoding Library updating mode (PGEL), which enables the cloudlet to be placed in appropriate positions. The simulation results show that the proposed strategy can obtain a near-optimal cloudlets placement scheme, and compared with other classic algorithms, this algorithm can reduce the execution time of WAs by 15.04%-44.99%.
Efficacy And Safety of Wenbu Zhibi Granule In Patients With Ankylosing Spondylitis: A Multicenter, Randomized, Double-Blind, Placebo-Controlled Trial
Authors: Zhang Helou , Yu Yang , Du Weibin , Wu Fengqing , Zheng Yang , Ren Conglin , Zhou Huateng , Wu Yijiang , Gao Yang , Ren Weifan , Quan Renfu 
Published: July 2021

Abstract
Background: Ankylosing spondylitis (AS) is a chronic disease in which the column is the main lesion. It is caused by a combination of genetic and environmental factors, mainly involving the axial skeleton, resulting in column rigidity and difficulty in movement, and there may be different degrees of eye, lung, cardiovascular, kidney and other organ damage. Long term treatment lacks in ankylosing spondylitis. Wenbu Zhibi granule (WZG) is a prescription handed down from the history of Chinese medicine for thousands of years, which is used to treat the pain of patients with AS and to prevent the further development of the disease. However, there is no scientific evidence based on clinical trial to evaluate the efficacy and safety of WZG for ankylosing spondylitis.Methods/design: We will conduct a multicenter, randomized, double-blind, placebo-controlled trial to evaluate the efficacy and safety of WZG in the treatment of AS. We will randomly assign 100 patients with active AS to two groups, treated for 16 weeks. The primary efficacy endpoint is the proportion of subjects who reached ASAS40 at 16 weeks from baseline, the secondary efficacy endpoint includes ASAS20 response rate, ASAS partial remission response rate, ASAS5/6 response rate, and change in Spondyloarthritis Research Consortium of Canada (SPARCC) MRI spine score, Bath Ankylosing Spondylitis Disease Activity Index (BASDAI), Bath Ankylosing Spondylitis Functional Index (BASFI), Ankylosing Spondylitis Disease Activity Score (ASDAS), linear Bath Ankylosing Spondylitis Metrology Index (BASMI), ankylosing spondylitis quality of life (ASQoL). In addition, the time points will be set as baseline, 2 weeks, 4 weeks, 8 weeks, 12 weeks, 16 weeks, 24 weeks and 48 weeks. Discussion: The results of this study will elucidate the efficacy and safety of WZG and provide an appropriate treatment option for patients with AS. Trial registration: ClinicalTrials.gov ID: ChiCTR2000041010. (Chinese Clinical Trail Registry, Registered 16 December 2020, http://www.chictr.org.cn)
p38-TFEB Pathways Promote Microglia Activation Through Inhibiting CMA-mediated NLRP3 Degradation in Parkinson's Disease
Authors: Chen Jialong , Mao Kanmin , Yu Honglin , She Hua , Zhang He , Liu Linhua , Wen Yue , li wenjun , Zou Fei 
Published: July 2021

Abstract
BackgroundParkinson’s disease (PD) is characterized by the degeneration of dopaminergic neurons in the substantia nigra pars compacta (SNpc), accompanied by chronic neuroinflammation, autophagy dysfunction and α-synuclein accumulation in the form of Lewy bodies. Previous studies showed that misfolded α-synuclein upregulates the inflammatory and autophagy dysfunction in microglial cell. The NLRP3 inflammasome signaling pathway plays a crucial role in the neuroinflammatory process in the central nervous system. However, the inter-relationship between autophagy deficiency and neuroinflammation induced by α-synuclein accumulation is not well understood.MethodsWe investigated the impact of p38-TFEB-NLRP3 pathways on neuroinflammation in the α-synucleinA53T PD models, using a combination of immunoblotting, immunofluorescence, immunocytochemistry, flow cytometry, ELISA, and a series of behavioral texts.ResultsIn the present study, we showed NLRP3 was degraded through chaperone-mediated autophagy (CMA) in microglia cell. Furthermore, p38-TFEB pathways inhibited CMA-mediated NLRP3 degradation in Parkinson's disease. Overexpress mice and BV2 cells with α-synuclein A53T mutant active P38, which inhibit the master transcriptional activator of autophagy, TEEB in BV2 cells. Notably, inhibition p38 had a protective effect on Parkinson's disease model, which depend on suppressing the activation of NLRP3 inflmmasome pathway. Importantly, both p38 inhibitor SB203580 and NLRP3 inhibitor MCC950 not only prevent neurodegeneration in vitro, but also alleviates movement impairment in α-synucleinA53T-tg mice model of Parkinson’s disease. Conclusion﻿Our research reveals an endogenous regulatory mechanism of NLRP3 turnover and microglia-dopaminergic neuron interaction, which may be a potential therapeutic strategy for Parkinson’s disease.
Understanding the impact of Commercial Banks Lending Rate on Economic Growth: An Empirical Evidence from Ghana.
Authors: Buabeng Emmanuel , Adabor Opoku , Nana-Amankwaah Elizabeth 
Published: July 2021

Abstract
The main objective of this paper is to investigate the impact of lending rate on economic growth in Ghana. To do this, we employ the autoregressive distributed lags model (ARDL) and the Toda and Yamamoto (1995) causal approach as estimation strategy. The estimates from the ARDL model suggest that ceteris paribus one percent increase in lending rate generates approximately 0.15 decrease in economic growth of Ghana in the long. In the short run, one percent increase in lending rate also generates approximately 0.112 percent decrease in economic growth. Contrary to the widespread belief that lending rate induce economic growth, we find that gross domestic product rather spurs lending rate, using Toda and Yamamoto (1995) causal approach. Our findings suggest that monetary authorities should embark on policy interventions that aim at taming lending rate towards growth enhancing targets. This will encourage individuals, firms and other institutions to borrow from commercial banks to increase investment and consumption to accelerate economic growth. Other policy interventions include strengthening inflation targeting policy to reduce and stabilize inflation while taming exchange rate, monetary policy and treasury bill rate towards economic growth enhancing targets.
Modeling and simulation of the enzymatic degradation of 2,4,6-trichlorophenol using soybean peroxidase
Authors: Cunha Alexandre Santuchi da , Junior Ardson dos Santos Vianna , Laurenti Enzo 
Published: July 2021

Abstract
The enzymatic degradation of organic pollutants is a promising and ecological method for the remediation of industrial effluents. 2,4,6-Trichlorophenol is a major pollutant in many residual waters, and its consumption has been linked to lymphomas, leukemia, and liver cancer. The goal of the present work is to comprehend the enzymatic degradation of 2,4,6-trichlorophenol using soybean peroxidase. Different assumptions for the kinetic model were evaluated, and the simulations were compared to experimental data, which was obtained in a microreactor. The literature pointed out that the bi-bi ping-pong model represents well the kinetics of soybean peroxidase degradation. Since it is a complex model, some reactions can be considered or not. Six different possibilities for the model were considered, regarding different combinations of the generated enzyme forms that depend on the hypotheses for simplifying the model. The adjustment of the models was compared based on different metrics, such as the value of the objective function, coefficient of determination and root-mean-square error. The process modeling was obtained by the mass balance of all the reaction components, and all the simulations were performed in MATLAB® R2015a. Reaction parameters were estimated based on the weighted least squares between the experimental data set and the values predicted by the model. The results showed that the data were better adjusted by the model that considers all the enzyme forms, including enzyme inactivation. Therefore, a better comprehension of the reaction mechanism was achieved, which allows a more precise reactor project and process simulation.
Galactomannan of Delonix Regia Seeds Modulates Cytokine Expression and Oxidative Stress Eliciting Anti-Inflammatory and Healing Effects in Mice Cutaneous Wound
Authors: Lima Iásly , Castro Rondinelle , Adjafre Beatriz , Sousa Skarlatt , de Paula Dayrine , Alves Ana Paula , Silva Paulo , Assreuy Ana Maria Sampaio , Mota Mario 
Published: July 2021

Abstract
Objective and designTo investigate the healing mechanism of Delonix regia galactomannan (GM-DR) in a mice model of excisional cutaneous wound. Materials and subjectsFemale Swiss mice were used in all treatments. TreatmentGM-DR (% 0.01-1) was topically applied to the wounds during 14 days.MethodsThe wound healing effect of GM-DR was evaluated by the following parameters: wound closure, clinical signs (hyperemia, edema, exsudate, nociception), oxidative stress markers (malondialdehyde – MDA, reduced glutathione - GSH), histopathological and histomorphometric analysis (collagenesis, blood vessels, polymorphonuclear, mononuclear, fibroblast/myofibroblast cells) and immunohistochemical (inflammatory growth factor mediators). ResultsGM-DR reduced wound area (7 - 14th day) and hypernociception (6 h - 5th day), leukocyte infiltration (2 -7th day), expression and levels of IL-1β (2th day), IL-6 (2th day), MDA (44% - 2th day), and increased fibroblast/myofibroblast, granulation tissue, collagen deposition, GSH (25 - 50%, 2-5th day), Transforming Growth Factor Beta (TGF-β) expression (7-10th day) and Smooth Muscle Alpha Actin (a-SMA) (7-14th day). Conclusions﻿GM-DR accelerates the mice healing process acting both in the inflammatory and proliferative phases.
Silencing the Triacylglycerol Lipase (TGL) Gene Decreases the Number of Apyrene Sperm and Inhibits Oviposition in Sitotroga Cerealella
Authors: Yan Wen-han , Wu Meng-Ya , Shah Sakhawat , Yao Yu-Chen , Elgizawy Karam Khamis , Tang Ning , Wu Gang , Yang Feng-Lian 
Published: July 2021

Abstract
Triacylglycerol lipase (TGL) is an essential lipid metabolism enzyme that also plays a critical role in energy metabolism; however, how it regulates other life processes is unknown. To investigate the functional role of TGL in moth reproduction, males Sitotroga cerealella were used as a model. The TGL gene was cloned and analysed. The results showed that the open reading frame of TGL was 1968 bp long and contained three conserved regions. TGL gene expression was higher in the larval and early adult stages than in the pupal stage, with the highest levels observed in the fat body, testis and accessory glands during the early adult stage. Moreover, after TGL in male adults was silenced through RNAi, the protein content in male accessory glands remained unchanged, and the spermatophore transferred into females mated with TGL -silenced males became small and empty; meanwhile, the number of apyrene sperm in the spermatophore was significantly reduced due to the reduction of apyrene sperm in males, which eventually led to the significant reduction of egg laying amount. All of the findings suggest that TGL regulates the amount of sperm in male moths as well as the morphology and quality of spermatophores transferred to females after mating with treated males, implying that TGL is critical for Sitotroga cerealella’s reproductive process.
Deformation of the nucleus by TGFβ1 via the remodeling of nuclear envelope and histone isoforms
Authors: Chi Ya-Hui , Wang Wan-Ping , Hung Ming-Chun , Liou Gunn-Guang , Wang Jing-Ya , Chao Pen-Hsiu Grace 
Published: July 2021

Abstract
The cause of nuclear shape abnormalities which are often seen in pre-neoplastic and malignant tissues is not clear. In this study we report that deformation of the nucleus can be induced by TGFβ1 stimulation in several cell lines including Huh7. In our results, the upregulated histone H3.3 expression downstream of SMAD signaling contributed to TGFβ1-induced nuclear deformation, a process of which requires incorporation of the nuclear envelope (NE) proteins lamin B1 and SUN1. During this process, the NE constitutively ruptured and reformed with no observable indications of DNA damage response. Contrast to lamin B1 which was relatively stationary around the nucleus, the upregulated lamin A was highly mobile, shuttling between the nucleus and cytoplasm, and clustering at the nuclear periphery. The chromatin regions that lost NE coverage formed a supra-nucleosomal structure characterized by elevated histone H3K27me3 and histone H1, the formation of which depended on the presence of lamin A. These results provide evidence that shape of the nucleus can be modulated through TGFβ1-induced compositional changes in the chromatin and nuclear lamina.
Nerve Growth Factor Promotes Lysyl Oxidase-dependent Chondrosarcoma Cell Metastasis by Suppressing miR-149-5p Synthesis
Authors: Tzeng Huey-En , Lin Syuan-Ling , Thadevoos Louis Anoop , Lien Ming-Yu , Yang Wei-Hung , Ko Chih-Yuan , Lin Chih-Yang , Huang Yu-Wen , Liu Ju-Fang , Fong Yi-Chin , Chen Hsien-Te , Tang Chih-Hsin 
Published: July 2021

Abstract
Background: The effects of Nerve growth factor (NGF) in chondrosarcoma are not confirmed, although NGF is capable of promoting the progression and metastasis of several different types of tumors. Here we aim to explore the role of NGF in chondrosarcoma and elucidate how NGF acts.Methods: Immunohistochemistry (IHC)-stained tissue samples from chondrosarcoma patients were stained with NGF and LOX antibodies. Cell migration was examined by Transwell migration and invasion assays. The expression levels of LOX, microRNA-149-5p (miR-149-5p) were measured by quantitative real-time polymerase chain reaction. LOX, PI3K, Akt, and mTOR protein expression were examined by Western blot assays. The interaction between LOX 3’-UTRs and miR-149-5p binding site was explored by luciferase assay. We established the orthotopic in vivo model of chondrosarcoma lung metastasis to further investigate the promoting effects of NGF in metastatic chondrosarcoma. Results: Here, we found that the levels of NGF and lysyl oxidase (LOX) correlated with tumor stage in patients with chondrosarcoma. NGF facilitated LOX-dependent cellular migration in human chondrosarcoma JJ012 cells, while overexpression of NGF enhanced lung metastasis in a mouse model of chondrosarcoma. NGF promoted LOX synthesis and cell migration by inhibiting miR-149-5p expression through the PI3K, Akt and mTOR signaling cascades. NGF appears to be a worthwhile therapeutic target in the treatment of metastatic chondrosarcoma.Conclusions: Our study has identified that NGF promotes LOX-dependent cell migration in human chondrosarcoma tissue by inhibiting miR-149-5p synthesis via the PI3K, Akt and mTOR signaling cascades
An Embedded Multiple Case Study: Using CFIR to Map Clinical Food Security Screening Constructs for the Development of Primary Care Practice Guidelines
Authors: Taher Sabira , Muramatsu Naoko , Odoms-Young Angela , Peacock Nadine , Fagen Michael , Suh Courtney 
Published: July 2021

Abstract
Background: Food insecurity (FI), the limited access to healthy food to live an active and healthy life, is a social determinant of health linked to poor dietary health and difficulty with disease management in the U.S. Healthcare experts support the adoption of validated screening tools within primary care practice to identify and connect FI patients to healthy and affordable food resources. Yet, a lack of standard practices limits uptake. The purpose of this study was to understand program processes and outcomes of primary care focused FI screening initiatives that may guide wide-scale program implementation.Methods: This was an embedded multiple case study of two primary care-focused initiatives implemented in two diverse health systems in Chicago and Suburban Cook County that routinely screened patients for FI and referred them to onsite food assistance programs. The Consolidated Framework for Implementation Research and an iterative process were used to collect/analyze qualitative data through semi-structured interviews with N=19 healthcare staff. Intended program activities, outcomes, actors, implementation barriers/facilitators and overarching implementation themes were identified as a part of a cross-case analysis.Findings: Programs outcomes included: the number of patients screened, identified as FI and that participated in the onsite food assistance program. Study participants reported limited internal resources as implementation barriers for program activities. The implementation climate that leveraged the strength of community collaborations and aligned internal, implementation climate were critical facilitators that contributed to the flexibility of program activities that were tailored to fill gaps in resources and meet patient and clinician needs. Conclusion: Highly adaptable programs and the healthcare context enhanced implementation feasibility across settings. These characteristics can support program uptake in other settings, but should be used with caution to preserve program fidelity. A foundational model for the development and testing of standard clinical practice was the product of this study.