bluuebunny/crossref_metadata_2025_split huggingface dataset:
An optimized method for high-quality DNA extraction medicinal fungi Mycoleptodonoides aitchisonii for whole genome sequencing
Authors: Riffiani R , Wada T , Shimomura N , Yamaguchi T , Aimi T 
Published: December 2021

Abstract
The extraction of pure high molecular weight DNA and collecting genomic DNA from fungi is difficult. This is because filamentous fungi has a sturdy cell wall, high protein and polysaccharide levels resistant to the usual DNA extraction procedures. A low-cost and reliable DNA extraction method was designed from Mycoleptodonoides. aitchisonii fit for whole-genome sequencing for identification and mapping of the A mating-type gene. Mycoleptodonoides. aitchisonii belongs to the Climacodontaceae family has pharmaceutical activities. In the present study, the mycelia of M. aitchisonii, which grew from the different concentrations of malt extract and minimal liquid media, have been compared systematically to determine the DNA extraction procedure resulted in DNA concentration with excellent purity and quality. The best protocol that resulted in good quality DNA was further validated using polymerase chain reaction amplification with a specific primer to amplify the homeodomain protein (Mahd2-18) gene that encodes a transcription factor. The method proposed DNA extraction using CTAB (Cetyl Trimethyl Ammonium Bromide) method and purify by commercial kit from mycelium grown in the minimal liquid media give the best result with the high concentration of DNA for whole-genome sequencing by Next Generation Sequencing and other applications.
Callus regeneration and polyploidy induction of Allium cepa L var. Bima Brebes using oryzalin
Authors: yun R Q A’ , Dinarti D , Husni A , Kosmiatin M 
Published: December 2021

Abstract
Polyploidy induction could increase shallot bulb-size to raise consumer preference and local shallot productivity. The research aimed to obtain an effective method of polyploidy induction on callus of onion (Allium cepa) var. Bima Brebes. The experiment was consisted of two experimental steps, which were callus induction of onion and polyploid induction of the callus. A 1×1 cm callus was treated by two drops of oryzalin with concentrations 0, 25, 50, 75, 100, and 120 μM. The ploidy level was identified based on morphological trait, stomatal analysis and DNA content using a flow cytometry. The results showed callus diameter, number of green spots, and number of shoots were decreased with increasing oryzalin concentration. The planlet leaves regenerated from oryzalin treated callus were darker than that of control. The flow cytometry analysis showed that planlets with 75 μM oryzalin was tetraploid, had longer and wider stomata than that of the control.
Biology and life table of Trichogramma chilotraeae, Egg parasitoids of Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae)
Authors: Sari L , Maryana N , Hidayat P 
Published: December 2021

Abstract

Spodoptera frugiperda is a species of armyworm that attacks maize crops globally and is a dangerous new invasive pest in Indonesia. Biological control, which employs natural enemies, is one method of controlling this pest. One of the natural enemies found in the field is the egg parasitoid of the Trichogrammatidae family. This study aimed to determine the reproductive capacity of the parasitoid on S. frugiperda eggs. The tested parasitoids were collected from S. frugiperda eggs on corn cultivations in Tenjolaya Village, Bogor Regency, West Java Province. The identification results showed that the parasitoid species used in this study was Trichogramma chilotraeae. The development periods of eggs, first instar larvae, second instar larvae, and pupae in the laboratory were 1.16 days, 1.18 days, 1.39 days, and 3.28 days, respectively. The reproductive capacity of T. chilotraeae on S. frugiperda eggs, referred to as the net reproduction rate (Ro), was 28.13, gross reproduction rate (GRR) was 30.43. the average time for one generation (T) was 9.07. The intrinsic rate of increase (r) was 5.54, and Doubling time (DT) was 1.88. Thus, the egg parasitoid of T. chilotraeae was not very effective as a potential biological agent for S. frugiperda.
Macrofungal identification and species richness in Cigelung Landscape and its influence on the presence of oil palm pathogens in PTPN VIII
Authors: Arko P F , Sudirman L I , Qayim I 
Published: December 2021

Abstract
Dungus Iwul Nature Reserved (CADI) is a remnant patch of tropical rainforest converted into plantations with neither past data nor study in macrofungi. In this article, we explored and identified macrofungal fruitbody in CADI and PTPN VIII Oil Palm Plantation (PTPN) around the nature reserve. The inventory was carried out with the opportunistic sampling methods assisted by the line intercept. Morphological characteristics were used to identify the macrofungal fruitbodies species. We found that the species richness in the study area stood at 120, with 70 species found in CADI, 23 species in PTPN planted in 2003, and 57 species in PTPN planted in 2004. These 120 species consist of 76 genera, 41 families, 11 orders, and four classes in Subkingdom Dikarya. Schizophyllum commune and Marasmiellus candidus in CADI and S. commune in PTPN planted in 2003 and 2004 were the species found with the highest relative frequency. Neither sign of basal stem rot on oil palm trees nor Ganoderma fruitbodies were found in both PTPN study locations, even though the fruitbodies were found in CADI. Further research is needed to determine if nature reserve could be a barrier against pathogens of monoculture oil palm plantation in a similar landscape model.
Bird visit to Ficus benjamina in two urbanization gradients in the tropics
Authors: Mardiastuti A , Mulyani Y A , Kusrini M D 
Published: December 2021

Abstract
Fig trees area believed to be important bird habitat in the tropics, including in urban areas. The research objective was to reveal the bird species that visited Ficus benjamina in the low and high urbanization gradients in the tropics. Data were obtained in IPB University Darmaga Campus (low urbanization) and Sentul City (high urbanization) in Bogor (West Java, Indonesia), through direct observations of four trees per site in the morning, midday, late afternoon, and night, totalling 276 observation hours. Total of 29 bird species visited F. benjamina trees (26 species in low urbanization, 12 species in high), mainly insectivores, nectarivores, and frugivores birds. Nine species were common in both sites, i.e., Spotted Dove, Plaintive Cuckoo, Fulvous-breasted Woodpecker, Small Minivet, Common Iora, Sooty-headed Bulbul, Yellow-vented Bulbul, Common Tailorbird, Scarlet-headed Flowerpecker, and Olive-backed Sunbirds. Nocturnal birds (Collared Scops Owl, Large-tailed Nightjar) were present in low urbanization, but absent in high urbanization site, so did kingfishers (White-throated Kingfisher, Collared Kingfisher) and some other urbanization-prone species. The high urbanization site was characterized by the presence of Eurasian Tree Sparrow at the fig tree. This study showed that F. benjamina has an important role for diurnal and nocturnal birds, even in the high urbanization site.
Mechanical properties of edible film based bacterial cellulose from sago liquid waste using starch as stabilizer
Authors: Yanti N A , Ahmad S W , Ramadhan L O A N , Walhidayah T 
Published: December 2021

Abstract
Edible film is a thin layer made of edible material as a packaging for food products. In the edible film production, required additional material that serves as stabilizer and plasticizer. This research aimed to determine the mechanical properties of the edible film-based bacterial cellulose from sago liquid waste using some types of starch as a stabilizer. The starches used as stabilizers in making edible films were corn, cassava, and sago starch. Mechanical properties were measured include tensile strength, elongation at break, elasticity (Young’s modulus), and water holding capacity (WHC). The results showed that the thickness of corn, sago, and cassava starch was 0.08 mm, 0.09 mm, and 0.11 mm, respectively. The mechanical properties of the edible film with corn, sago, and cassava starch as stabilizers namely a tensile strength (MPa) were 10.90; 15.90 and 61.92 respectively, elongation at break (%) were 8, 20, and 87, young’s modulus (MPa) were 13.48, 7.84 and 6.98, respectively and water holding capacity (g/g) were 34.26; 18.18 and 16.40 respectively. Therefore, the utilization of starch as a stabilizer in edible film can improve its mechanical properties.
Chitosan-Ag nanoparticle antifungal activity against Fusarium sp., causal agent of wilt disease on chili
Authors: Wahyuni S , Wibowo K R P , Prakoso H T , Bintang M , Siswanto  
Published: December 2021

Abstract
Chitosan is a polysaccharides compound derived from nature and has an amine group and a hydroxyl group that gives it unique physicochemical properties, which are polychationic and chelating agent. This shows that chitosan can be used as a stabilizer and reducer of Ag nanoparticles. Unlike other studies, chitosan that was used in this study was derived from Black Soldier Fly exuviae which has been through demineralization, deproteination, depigmentation, and deacetylation processes. This study aims to obtain an alternative environmentally friendly fungicide with Ag-chitosan nanoparticle formulation which has antifungal activity against Fusarium sp. that causes wilt disease in chili plants. We analysed particle size, functional group by FTIR, and antifungal test against Fusarium sp. on 1000, 750, 500, 250, and 100 ppm of chitosan-Ag nanoparticle. The chitosan-Ag nanoparticles have a particle size of 188.4 nm with a polydispersity index of 0.344. FTIR analysis showed that Ag+ ions are successfully grafted into the chitosan matrix. All the chitosan-Ag nanoparticle concentration tested showed significant results with negative and positive control, except the concentration of 100 ppm which is not significantly differ from negative control. The antifungal test showed a concentration of 750 ppm chitosan-Ag nanoparticles had a growth inhibition of 72.17%.
Isolation, characterization, and screening of antioxidant activity of endophytic bacteria from Leea Indica (Burm. F) Merr. Leaf
Authors: Arivo D , Mubarik N R , Rusmana I , Batubara I 
Published: December 2021

Abstract
Endophytic bacteria are bacteria that live within plant tissues and can produce the same compounds as their host. Leea indica, commonly known as bandicoot berry, is a medicinal plant with various bioactive compounds. A study on the diversity and antioxidant activity of endophytic bacteria from L. indica has never been reported. So this study is essential to conduct to explore the diversity and antioxidant activity of endophytic bacteria in the L.indica plant. The objective of the study was to isolate, identify, and screening of antioxidant activity of endophytic bacteria from L. indica leaf. We obtained 51 bacterial isolates with various of morphology. Microscopic identification showed that 44 isolates were categorized into Gram-negative (23 rod-shaped, 18 cocci-shaped, and 3 cocci bacilli-shaped isolates), and the other 7 isolates were categorized into Gram-positive (6 rod-shaped and 1 cocci-shaped isolates). The biochemical test showed that 100% of isolates have positive catalase, and 13.7% were suspected of having pathogenic activity on blood agar. The antioxidant activity showed that 17 isolates (more than 70%) had high inhibition by scavenging free radicals. It can be concluded that there are various endophytic bacteria isolated from L. Indica leaf and potentially producing antioxidant activity as their host.
Antibacterial activity of endosymbiotic fungi isolated from marine sponges collected from Kotok Kecil Island, Seribu Islands, Jakarta
Authors: Trifani R , Noverita  , Hadi T A , Sinaga E 
Published: December 2021

AbstractEndosymbiont fungi from marine sponges are a rich source of medicinally active compounds. Indonesia has a huge number of marine sponges. This research was conducted to determine the antibacterial potential of endosymbiont fungi isolated from marine sponges collected from Kotok Kecil Island, Seribu Islands. The screening was conducted with two methods, the modified GIBEX and disc diffusion. Species of marine sponges were isolated, namelyPetrosiasp.,Stylissa carteri, Cinachyrella australiensis, Callyspongiasp.,Petrosia nigrians, andStylissa massa, and obtained 9 isolates of endosymbiont fungi. The GIBEX test againstEscherichia coliandStreptococcus mutansshowed that ethyl acetate extract had strongest antibacterial activity. Disc diffusion test of ethyl acetate extract, five isolates had antibacterial activity againstS. mutansand four againstE. coli. The isolate that showed the strongest antibacterial activity was fromPetrosiasp. Which has an inhibition zone of 8.4 mm againstE. coliand 7.45 mm againstS. mutans. The main active compounds from the isolate ofPetrosiasp. are butylhydroxytoluene and phthalic acid di-(2-propylpentyl) esters. Based on this study, we concluded that the endosymbiont fungi of marine sponges are potential to be developed for further development as source of antibacterial agents.
Analysis of climatic variability and dengue hemorrhagic fever incidence at the Tsunami Area Banda Aceh City
Authors: Agustina E , Leksono A S , Gama Z P , Yanuwiadi B 
Published: December 2021

Abstract
The rebuilding of post-tsunami community residential an area in Banda Aceh City has created a new ecosystem and at the same time a public health problem. The construction of new settlements has an impact on the emergence of Aedes aegypti habitat. The post-tsunami climate factor is thought to be one of the factors that cause outbreaks of dengue hemorrhagic fever (DHF) cases every year in Banda Aceh City. This study aims to analyze the relationship between climatic variability with the DHF incidence cases from 2010-2020 at the tsunami area Banda Aceh City. The study uses monthly DHF case data obtained from the Banda Aceh City Health Office. Monthly climate data were obtained from the Meteorology, Climatology, and Geophysics Agency of Aceh Province. Spearman’s correlation analysis shows that temperature, humidity, rainfall, and wind velocity have a significant relationship with the DHF incidence cases. Humidity and rainfall are positively correlated with DHF incidence, while temperature and wind velocity shows a negative correlation with DHF incidence. This study shows that climate is one of the factors influential in the DHF incidence cases at the tsunami area Banda Aceh City.
Development and characterization of Simple Sequence Repeats (SSRs) markers in durian kura-kura (Durio testudinarius Becc.) using NGS data
Authors: Magandhi Mahat , Sobir  , Kusumo Yudiwanti W.E. , Sudarmono  , Matra Deden Derajat 
Published: December 2021

Abstract
Durian Kura-kura (Durio testudinarius Becc.) belongs to the Malvaceae family and is an endemic species of Borneo. Recently, genomic-based next-generation sequencing (NGS) approaches have been carried out for germplasm conservation and plant breeding programs. The NGS technologies allow plant genomes to be sequenced quickly and inexpensively and enable the efficient development of SSR markers through the in-silico approaches. This study aimed to develop and characterize simple sequence repeats (SSRs) from the assembled genome. The 1203929 scaffolds of the assembled genome were produced from the Ray assembler. The SSRs were identified and extracted using the MISA program produced 4315 sequences containing SSRs. The six motif repeats of SSRs were identified; consist of 431 sequences of dinucleotide (the most motif is AT), 3257 sequences of trinucleotide (the most motif is TTA), 516 sequences of tetranucleotide (the most motif is AAAT), 89 sequences of pentanucleotide (the most motif is ATTTT), 18 sequences of hexanucleotide and four sequences of heptanucleotide. The new SSRs markers will be used in further studies of genetic population of D. testudinarius and plant breeding programs.
Prospects of neem and citronella oil against Pseudococcus longispinus (hemiptera: Pseudococcidae) on Phalaenopsis
Authors: Hutapea D , Rahardjoa I B , Thamrin M 
Published: December 2021

Abstract
Synthetic insecticides are still widely used in gardens and greenhouses of ornamental crops to reduce populations of insect pests and preserve flower quality. The improper and unwise use of synthetic insecticides has caused various negative impacts on humans, insect pests, and the environment. Therefore, the quest for unharmed insecticides and environmentally friendly is being sought. The purpose of this study was to examine the effectiveness of botanical insecticide formulation of neem and citronella oil to control long-tailed mealybug Pseudococcus longispinus. Four insecticide concentrations of neem, citronella oil and its mixtures (0.5, 1, 1.5, 2 per cent) were tested for their effectiveness by adding each with Latron at 0.5% as an emulsifier. Bioassays were performed in the laboratory by spraying insecticides on Phalaenopsis leaves infested with P. longispinus groups of one size class, mostly represented by the third instar nymphs. The results revealed that all insecticides tested caused mortality after 24 hours. The combination of neem and citronella in 1.5% provided adequate protection by reducing P. longispinus populations by up to 86%. Thus, botanical insecticides can be recommended as a viable alternative to synthetic insecticides against P. longispinus which infested orchids.
Implementation Of Road Asset Management In Poland
Authors: Zofka Professor Adam , Maciejewski Andrzej 
Published: December 2021

Abstract
Road asset management (RAM) is a crucial aspect in the development of transportation network. RAM framework should be understood as a rational approach to business model for road authority, which dictates its business processes in a systematic and objective-based manner to ensure that strategic goals of an agency are reached. In a case of road network, such objectives are typically related to travel safety, time and comfort that should be maintained throughout the life cycle of the road assets at optimal costs. As such RAM enables road agencies to meet expectations of its customers to provide safe and reliable road network in effective and efficient way. Nowadays it becomes even more important to adopt performance-based and data-driven approach for road agencies considering current trends in transportation like connected, cooperative and automated mobility.
There are many aspects of RAM including legal, economic, technical and managing aspects. These aspects are inter-connected and nowadays agencies build and incorporate RAM system to establish a common denominator for all agency activities at all organizational levels. This paper discusses selected aspects of RAM in Poland. Since national road network in Poland is relatively new, it is particularly important to go over well designed and detailed implementation process. It should comprise numerous items including self-assessment and gap analysis, change strategy and transportation asset management plan (TAMP). This paper present update on these activities as well as presents future plans in order to establish modern, effective and sustainable RAM framework in Poland.
Planning Of State Road Renewal In The Context Of Territorial Reform
Authors: Skabs Salvis 
Published: December 2021

Abstract
In 2021 a new territorial reform will be carried out in Latvia, and the number of existing 110 local governments will be reduced to 42 regions (previous territorial reform in 2009 resulted in the decrease of the number of local governments from 522 parishes down to 110 local governments).
Each territorial reform has always regarded the importance of the network of state owned roads in ensuring successful operation of local governments, management of their territories and provision of municipal services to local inhabitants.
After the previous territorial reform carried out in 2009 there were 104 centres of local governments that were not connected with paved roads. At present only 59 such centres without paved road connections have remained, and improvements on roads serving these local governments is continued in the scope of annual improvement programmes financed from the state budget.
The Ministry of Environment and Regional Development within the scope of the new territorial reform in 2021 is preparing a new Investment Programme for Road Development in the Context of Territorial Reform. Respective road sections are identified by the administrations of planning regions in co-operation with local governments, the programme itself is compiled by the Ministry of Environment and Regional Development, but State Limited Liability Company “Latvian State Roads” is providing consulting on the choice of most efficient rehabilitation methods, preparation of technical documentation and potential construction costs.
Viastructura – A New Way Of Pavement Structure Design
Authors: Vaitkus Audrius , Kleizienė Rita , Karbočius Martynas 
Published: December 2021

Abstract
The catalogues of standard pavement structures are common way to design road pavements. In 2019, new regulation for the design of standard pavement structures KPT SDK 19 was issued in Lithuania. One of the new requirements require verification of layer thickness of high-class pavement structures. Such verification should be done by internationally approved mechanistic-empirical methods. In addition, it is recommended to use the same methods to adjust the layer thickness of the selected standard pavement structure for lower classes. These calculations are particularly applicable when the design load (ESAL) is at the lower or upper limit of the class range. Vilnius Tech Road Research Institute experts and outsource IT specialists spent two years for the design model ViaStructura development. Web software based on mechanistic-empiric approach include the boundary conditions, based on Austria, the United States and Germany experience and laboratory test results of construction materials. Materials can be selected from created database, which can be simply expanded with the new materials by the user. as Additional function allow comparison of separate designed pavement structures. The article present the concept of the ViaStructura model for the design of flexible pavement structures, reveals its main principles and advantages comparing to the pavement structure selection by the standard catalogue.