bluuebunny/crossref_metadata_2025_split huggingface dataset:
Red dragon fruit peel (Hylocereus polyrhizus) as feed biosupplement on performance of fattening rabbits
Authors: Prastiya R A , Yusuf AM 
Published: May 2021

Abstract
Peels of red dragon fruit (Hylocereus polyrhizus) as an agricultural by-product contain antioxidants and nutrients that can be used as one of the raw materials for animal feed. This study aims to determine the effect of various concentrations of peels of red dragon fruit (Hylocereus polyrhizus) as feed biosupplement on the performance of fattening rabbits. 20 rabbits were placed in individual cages and adapted to basal feed. They were fed with addition of the supplement concentration at 0% (DP0), 7% (DP7), 8% (DP8), and 9% (DP9) respectively for four weeks. Data were collected during the first, second, third and fourth weeks (including twenty-eight days) in the form of feed consumption, feed conversion, and weight gain. Data were subsequently analyzed using Analysis of Variance (ANOVA) with a 5% significance level. The results indicated the significance between the control treatment (DFP0%) and other treatments. The treatment with the addition of 9% biosupplement of the dragon fruit peel showed the highest performance improvement, with a mean value of feed consumption of 165.95 grams/day, mean of feed conversion of 3.61 and weight gain of 703.29 grams. In conclusion, this study demonstrates that the addition of fermented dragon fruit peel into the Rex rabbit feed can improve several aspects of rabbit performance.
Biosurfactant production and diesel-fuel removal by isolated bacteria from Kalimas Estuary, Madura Strait
Authors: Mustafa I , Oktaviani L T 
Published: May 2021

Abstract
This experiment was done to evaluate isolated bacteria in the production of biosurfactant and biodegradation of diesel-fuel. Diesel-fuel degrading bacteria were isolated from Kalimas Estuary, Surabaya using three-stage enrichment culture in Bushnell Haas Mineral Salts medium with 1% diesel fuel. Purified isolates were subjected to both oil-displacement and E24-emulsification tests to assess the high activity of produced biosurfactant. Selected isolates were next used in biodegradation analysis of diesel-fuel using a gravimetric total petroleum hydrocarbon test. The potential isolates were identified based on their 16S rDNA sequences. Twenty-eight purified isolates were obtained from the water samples and confirmed as gram-negative hydrocarbonoclastic bacteria. Among them, isolate K8 demonstrated the widest diameter on diesel-fuel displacement test by 3.5 cm of oil-free zone. It was also superior in emulsifying diesel-fuel with 9.4% of E24 index. Isolate K8 was capable of degrading 57% of 1 mL of diesel fuel within 14-day incubation. The isolate was potential in both producing biosurfactant and degrading diesel fuel. Isolate K8 was identified as Pseudomonas aeruginosa with 99.74% sequence similarity.
Coffee: ethnobotany, tourism and biodiversity conservation in East Java
Authors: Hakim L 
Published: May 2021

Abstract
Recent growth of third wave coffee movement provides opportunities for small holders coffee farmer and biodiversity conservation programs. The link of coffee consumption and biodiversity comes from several aspects, ranging from sustainable coffee farming to the development of rural tourism in coffee sites production. This paper will explore the ethnobotanical aspect of coffee, in which it is rarely described but crucial in the development of coffee in thirds wave coffee era. Secondly, this paper discuss the models of coffee involvement in tourism sectors. Thirdly, this paper describes the contribution of coffee cultivation under agroforestry approach to support biodiversity conservation. This paper shows that coffee rich in term of ethnobotanical aspects, ranging from cultivation to consumption. These aspects are crucial for the future development of coffee produced by smallholders farmers, especially for tourism development in thirds wave coffee era. Recent growth of café and coffee lovers become the crucial point to develop coffee farm tourism. This form of traveling is especially important to educate people about sustainable agricultural farming system, especially in coffee production. Coffee cultivations through agroforestry system represent the sustainable coffee culture cultivation. Coffee agroforestry systems provides opportunities as habitat for numerous small mammal, birds and insects. Coffee agroforestry therefore crucial to support biodiversity conservation. This paper present argument that linking ethnobotany, tourism and biodiversity conservation are the key to the future of sustainable coffee cultivation in East Java. This is especially important to support environmental conservation and smallholder farmers prosperity
Antibacterial activities of Etlingera flexuosa AD Poulsen (Zingiberaceae) from Central Sulawesi on Staphylococcus aureus and Escherichia coli
Authors: Pitopang R , Udayana R A D S , Pratiwi A D , Ananda M , Harso W , Ramawangsa P A 
Published: May 2021

Abstract

E. flexuosa which is endemic from Sulawesi is widely used by the local people for a lot of cultural activities. E. flexuosa fruit become the essential source to enhance the taste of fish dishes. The rhizome becomes a remedy to treat diarrhoea. This research aims to identify the antibacterial properties of E. flexuosa rhizome extract to two pathogen bacteria, Staphylococcus aureus and Escherichia coli. E.flexuosa sample were collected from Lore Lindu National Park, Indonesia. To detect the antibacterial activity, agar well diffusion was performed. The result showed that the extract of E. flexuose rhizome has antibacterial properties to inhibit the grow of Staphylococcus aureus and Escherchia coli because of the secondary metabolites, such as flavonoid, tannin, saponin, terpenoid and alkaloid
Potency of Cyperus rotundus bioactive compound against anti-apoptotic protein: an in silico approach
Authors: Nafisah W , Pinanti H N , Christina Y I , Soewondo A , Widodo  , Rifa’i M , Djati M S 
Published: May 2021

Abstract
The study aimed to analyze the potency of Cyperus rotundus bioactive compounds to inhibit the anti-apoptotic protein Bcl-2 and Bcl-xl by in silico approach. Ten bioactive compounds were used in this study, such as apigenin, aureusidin, cyperol, cyperusol A1, cyperusol B2, cyperusol D, luteolin, methyltartonic, quercetin, and scaberin. The 3D structure of ligands and protein was retrieved from PubChem and Protein Data Bank (www.rscb.org). The molecular docking analysis was done by AutoDock Vina in PyRx v.0.8. The results showed that the lowest binding affinity against bcl-2 was obatoclax as control ligand and followed by scaberin, aureusidin, luteolin, apigenin, and quercetin with binding affinity score - 7.4, -7, -6.9, -6.9, and -6.8 kcal/mol, respectively. Those ligands also found have the best binding affinity against Bcl-xl where apigenin, luteolin, and quercetin were -8 kcal/mol and lower than the binding affinity of obatoclax, aureusidin, and scaberin (-7.8, -7.8, and -7.3 kcal/mol, respectively). Based on the prediction of cytotoxic potential of drug-like compounds using Pass program showed the best cytotoxic activity of obatoclax against HT-29 cell line (pa>0.6), apigenin against Hs 683 (pa>0.5), luteolin against Hs 683 (pa>0.5), and quercetin against CWR22R (pa>0.5). In conclusion, the bioactive compounds of Cyperus rotundus exhibited a potential anti-cancer activity through the inhibition of Bcl-2 and Bcl-xl. Further study needs to justify the anti-cancer mechanism of Cyperus rotundus extract.
Diversity and the potency of indigenous bacteria in dengen fruit (Dillenia serrata), passion fruit (Passiflora edulis), and pineapple fruit (Ananas sp.) of South Sulawesi, Indonesia
Authors: Tenriawaru E P , Suharjono  , Ardyati T , Zubaidah E 
Published: May 2021

Abstract
Fruit plays the important role in plant conservation, public health, and welfare. The fruit is used by society as foodstuff, drinks, and condiments. The objectives of this study were to analyze the nutritional content in some fruits originated in South Sulawesi (dengen fruit, passion fruit, and pineapple fruit), to observe the metagenomic diversity and the correlation among nutritional content and alpha diversity, the potency of indigenous bacteria contains in the fruits. These fruits have historical and commercial value. Furthermore, dengen and pineapple are endemic fruits and almost extinct. Ripe fruit samples were obtained from public plantations in Luwu Raya, South Sulawesi Province, Indonesia. The indigenous bacteria in some fruits were observed by prepare each fruit juice. Each fruit juice was filtered using filter paper followed by nitrocellulose membranes pore of 0.45 and 0.20 μm respectively. Each fruit juice measured for its acidity degree using a pH meter and the nutritional contents using the titration methods. The chromosomal DNA of bacterial cells had extracted by FastDNA Spin Kit (MPBIO), and partial of 16S rDNA amplified with 341f-806r primers, and it analyzed by Illumina platform. The sequence of 16S rDNA was analyzed by MUSCLE v.3.8.31, QIIME v.1.7.0, R v.2.15.3, and SPSS v.20 software. The results showed that pineapple fruit has the highest sugar, reducing sugar, starch, and amylose content, while dengen fruit has the highest vitamin C content. The combination of sugars and vitamin C content may influence the dominant microbial genera. Dengen fruit was dominated by Phylum Proteobacteria and it dominated by Genus of Acetobacter, Gluconobacter, and Komagataeibacter. Passion fruit and pineapple fruit were dominated by Phylum Firmicutes and Genus of Weissella. Genus of Acetobacter, Gluconobacter, and Komagataeibacter able to produce acetic acid, while Weissella is known as a lactic acid producer.
Evaluation of water quality using plankton diversity in Sumber Maron River District Gondanglegi Kulon East Java-Malang, Indonesia
Authors: Tawati F , Risjani Y , Djati M S , Leksono A S , Yanuwiadi B 
Published: May 2021

Abstract
The diversity of species in the ecosystem is directly related to abundance or adaptation, and the presence of plankton in the ecosystem is useful for assessing the state of pollution. An assessment of phytoplankton and zooplankton has been carried out at nine sites of the Sumber Maron River, which will help to understand the biodiversity indicators of the river water. Sediment Samples were taken from the lower river and placed in plastic bags with a volume of about one kg, during the rainy and dry season of 2016, phytoplankton diversity was observed in 13 Genus while the diversity of zooplankton included 10 Genus. Thus the present study gives an indication of the diversity of phytoplankton and zooplankton which can be useful as data to determine the status of water quality of the Sumber Maron River
The study of spectral uv-vis on coconut water as flour solvent of soybean, black rice and red rice
Authors: Rahmawati K , Kristanti N D , Sandriakana  , Sumitro S B , Widyarti S 
Published: May 2021

Abstract
Coconut water (CW) is a nutritious drink containing highly complex components. Therefore, CW has an interesting unique physicochemical nature to investigate. This study aims to know the UV-Vis spectra of CW as flour solvent of soybean, black rice, and red rice. Each flour was homogenized with ratio of 1:10 (w/v) in fresh CW (f-CW) or heated-CW at 50°C (h-CW). The homogenized flour was centrifuged at 4500 rpm, 4°C, for 5 minutes. The supernatant was analyzed based on UV-vis spectra, pH, and turbidity. Flour that dissolved in CW shows peaks only in the UV spectrum, whereas the flour dissolved in water shows peaks in the UV-vis spectrum. Generally, flour component more dissolves in the CW than in the water. Turbidity value of flour dissolved in the CW lower than those dissolved in the water. The flour dissolved in the CW slightly acid than in the water. The flour dissolved in h-CW shows a slight decrease in absorbance value compared to the f-CW, meanwhile, flour dissolved in water generally have relatively the same spectrum profile.
Molecular docking study to reveal Morinda citrifolia fruits as a novel EGFR inhibitor for anticancer therapy
Authors: Agustina D W , Wahyuningsih M D , Widyarti S , Rifa’i M 
Published: May 2021

Abstract
Lung cancer is the leading cause of cancer death in the world and represents a major public health burden. Epidermal Growth factor Receptor (EGFR) as targets for hormonal cancer therapy shown to decrease cell proliferation and tumor growth. Indonesian People usually consumed Health tonic from Morinda citrifolia fruits known as Noni juice. Morinda citrifolia fruits have been known for a therapeutic benefit such as antidiabetic, anticancer and pain. This study aims to investigate molecular interaction between Morinda citrifolia fruits compound with the active site of ERb antagonist and EGFR tyrosine kinase inhibitor for lung cancer therapy. Morinda citrifolia fruit compounds were retrieved from Dr. Duke’s Phytochemical and Ethnobotanical Databases (https://phytochem.nal.usda.gov) meanwhile ligand was retrieved from PubChem and 3D protein from PDB. The first step was ligand and receptor preparation before docking process using VegaZZ. Molecular docking process was calculated using Autodock Vina in PyRx v.0.8. The Result of this study reveals 12 major compounds of Morinda citrifolia fruits Anthraquinone, Benzo acid, Beta carotene, Eugenol, Limonene, Quercetin, Rutin, Scopoletin. Based on the molecular binding analysis, Quercetin are best docking complex with EGFR. The result showed that Quercetin is the best antagonist for EGFR indicated by the highest affinity (-8.6 Kcal/mol). It can be concluded that Quercetin in Morinda citrifolia is main compound in noni fruits that act as anticancer agent.
The effect of cutting the bulbil-porang (Amorphophallus muelleri) on its germination ability
Authors: Harijati N , Ying D 
Published: May 2021

AbstractBulbil, an aerial tuber specifically found on porang, is one tool for multiplication of porang (Amorphophallus muelleri).. The aim of this study was to determine the effect of bulbil cutting on the number of tubercles, the proportion of black and white tubercles, the potential for shoot production, the number and height of shoots produced from both cutting (2 and 4 cuts) which appeared in the abaxial or adaxial parts, and the direction of shoot growth. This study was designed using a completely randomized design, each unit of observation was four-replicated. The obtained data were analyzed using Anova, Tukey or independence T-test. The results showed the number of shoots from the whole bulbil, bulbil cut (two or four) differed significantly. The highest number of shoots obtained from bulbil cut was 4 which was 9 shoots. Those highest number of shoots was supported by the highest white tubercle. However, the potential for shoots to emerge from both whole and cut bulbils were almost the same, the length of the shoots did not differ significantly between them as well. The ratio between white and black tubercles seemed to affect the number of shoots, the high ratio produced more shoots. The tubercle ratio from intact bulbils, cut-2 bulbils and cut-4 bulbils tend to increase but not significantly different. The length of shoots that appear from the adaxial and abaxial parts was not different as well. All shoots from both the whole bulbil and cut bulbil grew upwards, none of which grows horizontally or towards the earth.
Modelling Casuarina junghuhniana dispersal in Tengger sea of sands of Bromo Tengger Semeru National Park using cellular automata
Authors: Rahardi B , Indriyani S , Hakim L , Suryanto A 
Published: May 2021

Abstract
The growing of population of a pioneer species may show a unique behavior. Casuarina junghuhniana is reported as a pioneer species, this is because of its tolerance to adverse environment and transform it to become inhabitable for other organism. A comprehensive method involving field observation, the use of mathematical model and computation to understand plants behavior still needs to be developed. This paper is attempting to show a method to build a model for showing unique dispersal behavior of Casuarina junghuhniana. A relatively straightforward use of Fisher’s reaction diffusion equation, cellular automata based modeling and programming can show behavior easier to understand on a grid space. Some assumption must be used to represent the C. junghuhniana dispersal. As it is known that C. junghuhniana can reproduce both vegetatively and generatively, and both were assumed to have different dispersal behavior. Vegetative reproduction using shoots emerging from roots has pattern to spread close to each other. While generative reproduction by seeds which is lightweight has possibility spread further with the help of wind. The total model area of 1km 2 was divided into 300 × 300 cells which represents 1 km 2 area. Cellular automata apply the same rule to each cell and the result was observed from the pattern of cells interaction. A simple program was developed using Python with Jupyter Notebook an interactive computing environment. The number of iteration was limited before observed simulation reached one of area boundaries. The simulation running time was greatly reduced using Graphical Processing Unit (GPU) parallel processing. NUMBA was used as an interface between python and Compute Unified Device Architecture (CUDA), the GPU programming API. The simulation showed behavior of the trees spread downward along the slope. The wind direction simulation with additional data from wind rose available from internet was also demonstrated in the simulation. Modeling dispersal with influence from wind showed more sporadic pattern at further distance from originating parents cluster. The trees in the simulation showed clusters in dispersal pattern when rely more on generative reproduction. When the trees rely on vegetative reproduction by roots shoots, the population showed more like continuous cover. When concerning real 3 dimensional aspect, this simulation still can not distinguish vertical (z axis) aspect. It means two points would be seen as close to each other although they actually have far vertical distance. At this stage, the simulation has not yet been optimized to easily convert input from the wind rose. The wind information still needs to be written as code into the program.
Caffeine-degrading endosymbiont fungi isolated from Hipothenemus hampei. Ferr as pre-analysis caffeine tolerance ability of coffee berry borer
Authors: Purwatiningsih  , Ubaidillah S , Setyati D , Wiyono H T 
Published: May 2021

Abstract
The Coffee Berry Borer (CBB), Hypthenemus hampei Ferr., is the most destructive pest of coffee berry due to its ability to bore and complete their entire life cycle inside of the coffee berry. The detoxification mechanism of CBB ability to lives inside of coffee berry containing high natural insecticide compound such as caffeine is a very interesting study and it may lead to be an advantaged for CBB controlling methods. Isolation and screening of endosymbiont fungi from CBB using 0.5% caffeine plate agar as a minimum nutrient as well as a selective medium were obtained 10 isolates consist of 6 isolates of yeasts and 4 filamentous fungi which positively degraded caffeine content through their formed diameter of the colony. The presenting of these several caffeine-degrading endosymbiont fungi could be as pre-explanation about the caffeine tolerance mechanism of CBB.
Community forest management of Arabica coffee based on local and modern ecological knowledge
Authors: Tridakusumah A C , Supyandi D , Arari M , Sukayat Y 
Published: May 2021

Abstract
Coffee farmers, members of Kramatjaya Forest Farmers Group in Garut Regency West Java, use Perhutani’s land to cultivate Arabica coffee. Apart from being a conservation area, this land is also arable volcanic soil and requires proper management that prioritizes coffee agribusiness aspects as well as ecological aspects, based on local and modern knowledge. This study implemented a knowledge management approach to reveal an understanding of local and modern ecological knowledge management in community coffee business practices. Empirical data were obtained through in-depth interviews and FGD. The research results show that for the last 10 years farmers have been utilizing Perhutani’s land to cultivate Arabica coffee by implementing modern coffee equipment and also adopting local ecological knowledge in each sub-system. Local knowledge in the production sub-system is demonstrated by terracing systems. In the sub-processing system, local knowledge is used in coffee processing using traditional methods, along with uses modern equipment. The study also shows that knowledge generation derived from external and internal sources, knowledge transfer came from the role of community leader and field school training, and knowledge application of coffee processing used modern technology through community leader networks.
Policy study on the management of forest with special purposes
Authors: Agustina A , Suryandari R T , Wicaksono R L 
Published: May 2021

Abstract
Forest with Special Purpose (FwSP) is designated to advance research, teaching, and training. These FwSPs are usually managed by universities or training centers. This forest management is regulated by the Ministry of Environmental and Forestry (MoEF), while universities are entities under the Ministry of Education. FwSPs management follows regulations under MoEF. In this line of thought, in managing the FwSP, universities should comply with regulations released by MoEF. One specific regulation on FwSP management is MoEF’s Regulation FwSP No. 15/2018, in which the formal regulations, limitations, and physical development related to the activities of FwSP are covered and detailed. This paper is aimed at presenting the problems face by universities managing FwSP. Collecting data from FwSP in Java Island, using qualitative methods, the findings are expected to appeal to MoEF in understanding the practical problems related to FwSP management. The in-depth interview result that some conflicts of interest and problems are manifested because of the unclear information provided in interpreting some aspects of the regulations. Especially in utilizing and maintaining FwSP. Another challenge faced by universities managing FwSP is the budgeting system, which brings burdens for FwSP in securing the funding to manage and protect forest sustainability properly.
Describing and visualizing the progress of ethnozoology in Indonesia by using VOSviewer
Authors: Mardiastuti A , Masy’ud B , Ginoga L N , Sastranegara H , Sutopo  
Published: May 2021

Abstract
As a science, ethnozoology is relatively less popular compared to ethnobotany, although local people in Indonesia have been practicing ethnozoology for thousands of years. This paper aimed to describe the progress of ethnozoology in Indonesia, specifically about research topic within ethnozoology, taxa mostly harvested by the local people, as well as institutions that mostly conduct ethnozoological research. An intensive literature study of the published papers in the last 20 years was performed. Data were analyzed by using VOSviewer. Of the 53 published literatures, VOSviewer showed that the research topic of ethnozoology based on keywords has 16 clusters with 464 links and mostly related to traditional medicine (15 occurrences; 62 total link strength) and reptiles (13 occurrences; 54 total link strength). Among vertebrates, the most widely-utilized taxon was herpetofauna (reptiles and amphibians) which were included in 44 references. The institutions that have been conducting research on this topic mostly were IPB University (15 refs), Tanjungpura University (7 refs), and Bangka Belitung University (4 refs). Although VOSviewer was meant to construct and visualize bibliometric networks based on authors/co-authors and their affiliations, citation/co-citation, and bibliographic coupling, this software can also be used to describe and visualize specific themes or topics as well.