bluuebunny/crossref_metadata_2025_split huggingface dataset:
Not hurting students’ feelings: The impact of a specialized training on teacher mental health literacy in central Sri Lanka
Authors: Ginige P. , R.U Perera , S.C. Arambepola , P.R Kuruwita , N.D. Gunawardena 
Published: February 2021

Abstract
BackgroundGlobally 10-20% of children and adolescents are suffering from mental health disorders. Improving mental health literacy (MHL) of teachers supports early detection and referral of children with mental health problems.AimTo develop and conduct a training program to improve teachers’ MHL, “Sisu Sitha Noridawa (SSN)” (not hurting students’ feelings) in central Sri Lanka (SL) and to explore the effect of the intervention on teacher MHL.Method251 teachers participated in the study. Psychiatrist authors first facilitated small group discussions on ten case vignettes of common child psychiatry problems and then large group discussions using mini lectures and a handbook. Pre and post-tests were conducted to assess knowledge and attitudes on mental health problems of school children. The scores were analyzed using, Paired T-test in SPSS 17.ResultsThe mean score differences of pre and posttest in both knowledge and attitude components showed statistically significant differences with p values <0.001 (confidence interval of 95%) and Cohen’s d values >0.2 in each, indicating the training had a significant positive impact on improving both the knowledge and attitude about mental health.ConclusionThe training program had a significant positive impact on improving the MHL of participant teachers on common child and adolescent mental health problems. The sustainability of achieved effects and their application in classroom need exploration in a long-term study. SSN is a feasible and effective training program to be included in the existing teacher-training curriculum with in-service booster SSN programs, in a middle-income country like SL.
Maternal Depression, Adverse Childhood Experiences, and Social Support in Relation to Gestational Diabetes Risk
Authors: Versteegen Margaret E. , Bozlak Christine T. , Larkin Heather , Appleton Allison A. 
Published: February 2021

Abstract
Purpose

Psychosocial factors are of increasing interest as potential influencers in disease development. This study explores associations between gestational diabetes mellitus (GDM) and maternal depression, adverse childhood experiences (ACEs), and social support, in response to emerging evidence in these areas.
Methods

An observational, prospective cohort study (AIMS) served as the source of secondary data for this study. Participants included 300 pregnant women aged 18–40 years at an upstate New York prenatal care clinic, who completed a set of self-report questionnaires assessing exposures and stressors both during and prior to their pregnancy. Data were also abstracted from infant and maternal medical records.
Results

Logistic regression modeling estimated the odds ratios (ORs) of developing GDM in relation to psychosocial factors. There was a significant association between depression and GDM (OR = 2.85, 95% CI: 1.15, 7.06), which persisted in the model adjusted for age and BMI (aOR = 3.19, 95% CI: 1.25, 8.10). No significant associations were found between ACEs or social support with GDM.
Conclusions

Study findings support an association between maternal depression and GDM development. This study underscores the need for additional research on psychosocial factors and connections to health risks.
Association between Handgrip Strength and Depression in Older Adults - A Systematic Review and a Meta-Analysis
Authors: Zasadzka Ewa , Pieczyńska Anna , Trzmiel Tomasz , Kleka Paweł , Pawlaczyk Mariola 
Published: February 2021

Abstract
Background: Depression remains an important health problem among older adults. Disorders in older age result from the accumulation of various factors, chief among them somatic diseases, stressful life events, social isolation, unfavorable social attitudes towards older people, declined cognitive function, malnutrition, polypharmacy. Depression may be associated with the deterioration of physical fitness, whose chief indicator is hand grip strength (HGS). The aim of the study was to investigate the relationship between depression and HGS among older populations using the available literature. Methods: PubMed, Web of Science and Science Direct databases were searched. The inclusion criteria were as follows: written in English and published after 2009, subject age: ³60 years, HGS measured using a hand dynamometer, assessment of the depressive symptoms using a validated tool. The following articles were excluded: studies conducted among institutionalized subjects and/or populations with a specific disease. Results: The total combined effect of 33 results presented in 16 studies included in the meta-analysis, converted to the correlation coefficient, was OEr =-.148(SE = .030, 95%CI:-.206 – -.091), indicating a weak, negative correlation between HGS and depressive symptoms. Conclusion: The review of the literature and the meta-analysis demonstrated a relationship between low muscle strength measured with the HGS test and intensified depressive symptoms in older populations. Bearing in mind that depression is often unrecognized or underdiagnosed among older patients, lowered muscle strength in older subjects, should be an important sign for physicians and physiotherapists and an incentive to screen them for depression.
Chemical variability in vyacheslavite, U(PO4)(OH): Crystal-chemical implications for hydrous and hydroxylated U4+, Ca, and REE phosphates
Authors: Steciuk Gwladys , Škoda Radek , Dillingerová Veronika , Plášil Jakub 
Published: February 2021

Abstract
Particularly interesting chemical variability in the U4+ phosphate mineral vyacheslavite from Menzenschwand (Germany) has been discovered and investigated by means of electron-diffraction and micro-chemical methods. Suggested variability comprises the elevated contents of calcium and rare-earth elements (REEs or Ln). Based on the crystal structure refinement from 3D electron diffraction data, the structural formula of Ca-rich vyacheslavite studied is U0.895Ca0.105 PO4(OH)0.790(H2O)0.210. In general, such compositional variability involving Ca2+ can be expressed as U1–x CaxPO4(OH)1–2x(H2O)2x. Based on detailed electron-probe microanalysis, regions extremely enriched in Y and Ln have been discovered, characterized by the contents up to 11 wt% of Y2O3 and ~4.5 wt% of Ln2O3. In addition to the above-mentioned substitution mechanism, substitution involving Y and Ln can be expressed as U4+ + OH– → REE3+ + H2O. Although the structure refinement has not provided direct evidence of H2O in the studied nano-fragments of vyacheslavite, the presence of H2O and its substitution at OH– sites is a reasonable and necessary charge-balancing mechanism. One H atom site was located during structure refinements; however, an additional H-site is only partially occupied and thus was not revealed from the refinement despite the high-quality data. Substitutional trends observed here suggest possible miscibility or structural relationship between vyacheslavite, rhabdophane, and ningyoite that may depend strongly on OH/H2O content, considering that all crystallize under similar paragenetic conditions.
Water footprint assessment of viscose staple fiber garments
Authors: Qian Weiran , Zhu Juxiang , Chen Fangli , Ji Xiang , Wang Xiaopeng , Wang Laili 
Published: February 2021

Abstract
The viscose fiber industry forms a large part of the textile industry and is a typical water consumption and wastewater discharge industry. As a tool to quantify environmental impacts in terms of water resources, the water footprint assessment (WFA) is a control method for the textile and apparel industry to measure water consumption and wastewater discharge. In this study, the water footprints of viscose staple fiber blouses and blended men's suits were comprehensively evaluated based on the ISO 14046 standard and the life cycle assessment (LCA) polygon method. The WFA results from our study indicate that the production stage of viscose staple fiber garments has the most significant water resource environmental load. Specifically, the water footprint related to the production of viscose staple fiber for three types of clothing accounted for more than 50% of the total water footprint, with men's 100% viscose staple fiber suits having the largest impact on water resources and the environment. Furthermore, our results indicate that the water alkaline footprint is primarily influenced by the viscose staple fiber production as well as the dyeing and finishing processes. NaOH and Na2CO3 are the main pollutants that caused the water alkaline footprint. In addition, the water ecotoxicity footprint was the major driving factor of water resource environmental load. Zn2+ is the main pollutant that caused the water ecotoxicity footprint.
Dissolved organic matter byproducts from combined low-level alkaline and mechanical treatment of sludge: species, and their bioavailability
Authors: Tian Qing , Jiao Pengbo , Lu Haoliang , Zhu Yanbin , Wolfgang Sand 
Published: February 2021

Abstract
Low-level alkalinity (pH 9–10) coupled with ultrasonic or mechanical cutting with different energy input for obtaining carbon sources were tested for sludge pretreatment process before anaerobic sludge digestion. The differences between the primary sludge (PS) and waste activated sludge (WAS)-derived dissolved organic matter (DOM) species were evaluated for their bioavailability and affinity (in the form of amino acids) to the bio-nutrient removal (BNR) biomass. Soluble microbial by-product-like substances as the predominant DOM components in the raw PS and WAS increased by 23 and 22%, respectively, after low-level alkaline treatment (pH 9–10) and ultrasonication. In addition, the protein components were degraded further as free amino acids (FAAs). The sludge-derived aspartate, glutamate, followed by arginine were the most commonly used FAAs by the BNR biomass. The pattern of recovering this special sludge-derived carbon source to enhance P removal and recovery in the BNR process is depicted.
Gene Therapy of Mucopolysaccharidosis Type I Mice: Repeated Administrations and Safety Assessment of pIDUA/Nanoemulsion Complexes
Authors: Fraga Michelle , Schuh Roselena Silvestri , Poletto Édina , de Carvalho Talita Giacomet , França Raqueli Teresinha , Pinheiro Camila Vieira , Baldo Gilherme , Giugliani Roberto , Teixeira Helder Ferreira , Matte Ursula 
Published: February 2021

Background:
Mucopolysaccharidosis type I (MPS I) is an inherited disorder caused by
α-L-iduronidase (IDUA) deficiency. The available treatments are not effective in improving all
signs and symptoms of the disease.

Objective:
In the present study, we evaluated the transfection efficiency of repeated intravenous administrations of cationic nanoemulsions associated with the plasmid pIDUA (containing IDUA gene).

Methods:
Cationic nanoemulsions were composed of 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE), 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-(amino[polyethylene glycol]-2000) (DSPE-PEG), 1,2-dioleoyl-sn-glycero-3-trimethylammonium propane (DOTAP), medium chain triglycerides, glycerol, and water and were prepared by high-pressure homogenization and were repeatedly administered to MPS I mice for IDUA production and gene expression.

Results:
A significant increase in IDUA expression was observed in all organs analyzed, and IDUA
activity tended to increase with repeated administrations when compared to our previous report
when mice received a single administration of the same dose. In addition, GAGs were partially
cleared from organs, as assessed through biochemical and histological analyzes. There was no presence
of inflammatory infiltrate, necrosis, or signs of an increase in apoptosis. Furthermore, immunohistochemistry
for CD68 showed a reduced presence of macrophage cells in treated than in
untreated MPS I mice.

Conclusion:
These set of results suggest that repeated administrations can improve transfection efficiency of cationic complexes without significant increase in toxicity in the MPS I murine model.
Efficacy and Safety Outcomes of Short Duration Antiplatelet Therapy
with Early Cessation of Aspirin Post Percutaneous Coronary Intervention:
A Systematic Review and Meta-analysis
Authors: AL-Obaidi Firas R. , Hutchings Hayley A. , Yong Andy S.C. , Alrubaiy Laith , Al-Farhan Hasan , Al-Ali Mohammed H. , Al-Kinani Tahsin , Al-Myahi Mohammed , Al-Kenzawi Hussein , Al-Sudani Nazar 
Published: February 2021

Background:
The optimal duration of dual antiplatelet therapy is a matter of ongoing research.
Clinical studies are assessing the optimal duration with the most favourable risk to benefit
ratio. The efficacy of P2Y12 receptor inhibitors comparable to aspirin in preventing recurrent
ischaemic events in patients with coronary artery diseases.

Objectives:
To investigate the outcomes of short-duration dual antiplatelet therapy after PCI with
early discontinuation of aspirin while maintaining patients on P2Y12 inhibitor through systematic
review and meta-analysis of available literature.

Methods:
We systematically searched PubMed, Cochrane Central Register of Controlled Trials
(CENTRAL), and ClinicalTrials.gov. We included randomized controlled studies that measured
clinical outcomes of efficacy (mortality and ischaemic events) and safety (bleeding) of short and
standard-duration dual antiplatelet therapy. The protocol of this study was registered in the international
prospective register of systematic reviews PROSPERO registry (CRD42020171468).

Results:
Four randomized controlled trials were included; GLOBAL LEADERS, SMARTCHOICE,
STOPDAPT-2, and TWILIGHT. The total number of patients was 29,089. The safety
outcomes showed a significant reduction in major bleeding events with short-duration dual antiplatelet
therapy; the risk ratio was 0.61 (95% CI 0.38-0.99; z=2,00, p=0.05). There was no difference
between short and standard-duration dual antiplatelet therapy regarding efficacy outcomes (all-
cause death, major adverse cardiovascular events, myocardial infarction, stroke, and stent thrombosis).

Conclusion:
Short-duration dual antiplatelet therapy followed by P2Y12 inhibitor monotherapy after
PCI is a feasible option and can be adopted, especially in patients with a high risk of bleeding.
Ultra-Performance Liquid Chromatography Coupled with a Triple Quadrupole Mass Spectrometric Method for the Quantification of Antiepileptic Drugs Methsuximide and Normesuximide in Human Plasma and its Application in a Pharmacokinetic Study
Authors: Rajendran Karthik , Anoop Karthika , Nagappan Krishnaveni , Sekar Genguchetti Mohan , Rajendran Sankham Devendran 
Published: February 2021

Background:
Extensive therapeutic drug monitoring needs an analytical method for
efficient and sensitive quantification of analytes of interest in clinical pharmacology.

Objective:
A rapid, robust, sensitive and simple UPLC-MS/MS method to quantify
Methsuximide (Ms) and N-desmethyl methsuximide/Normesuximide (MsMET) in human plasma
was optimized, developed, and validated for application in a pharmacokinetic study.

Method:
Reverse phased chromatography was performed using Zorbax SB-C18, 4.6 x 75 mm.,
3.5 μm as stationary phase, methanol and 0.1% formic acid (60:40 v/v) as mobile phase which
was delivered isocratically at a flow rate of 0.9 mL/min. The sample injection volume was 5 μL.
Mass spectrometric quantification of the analytes was performed using positive electrospray ionization
as mass interface along with multiple reaction monitoring (MRM) as acquisition mode.

Results:
The selected mass transition ions for analyte, metabolite and its respective internal
standards are as follows, precursor ion (m/z) and product ion (m/z): Ms (204.06 and 119.02),
MsMET (190.05 and 119.82), Ms internal standard (MsIS) (209.17 and 124.02), and MsMET internal
standard (MsMETIS) (195.09 and 124.16), respectively. The current method was found to be
linear for Ms (60.720-6043.800 ng/mL) and MsMET (60.389 - 6010.800 ng/mL) with r2 values not
less than 0.999. The mean recoveries of all analytes ranged between 71.37 and 86.38 percentage.
Conclusion: This method was validated in accordance with USFDA’s bioanalytical guidelines.

Conclusion:
This method was validated in accordance with USFDA’s bioanalytical guidelines.
This method could be applied for a routine analysis of Ms and MsMET in clinical pharmacological
practice.
Determination of Total Arsenic, Soluble Arsenic, Total Mercury and Soluble Mercury for a Realgar and Cinnabar-containing Traditional Chinese Medicine Compound Niuhuang Xiaoyan Capsule by Semi-bionic Extraction- ICP-MS
Authors: Xu Bo-Yang , Zhu Si-Qi , Cheng Xian-Xin , Xu Yu , Liu Ji-Wu 
Published: February 2021

Background::
Compound Niuhuang Xiaoyan capsule is a realgar and cinnabar-containing traditional Chinese medicine, the contents of total arsenic and total mercury are found over regulation defined limits. However, only a small amount of arsenic in realgar and mercury in cinnabar are dissolved in the gastric juice, so the determination of soluble arsenic and soluble mercury is more significant than that of total arsenic and total mercury in Compound Niuhuang Xiaoyan capsule.

Objective: :
To study the contents of total arsenic, total mercury, soluble arsenic and soluble mercury in Compound Niuhuang Xiaoyan capsule.

Method::
Microwave digestion and semi-bionic extraction were used to pretreat the samples of Compound Niuhuang Xiaoyan capsule, and the inductively coupled plasma mass spectrometry method was established to determine the contents of total arsenic, total mercury, soluble arsenic and soluble mercury in Compound Niuhuang Xiaoyan capsule. The accuracy of the established method was further evaluated by using a certified standard reference material prepared from dried citrus leaves (GBW10020(GSB-11)).

Results: :
The spiked recoveries were within 95-105 %. The R.S.D% of repeatability (N=6) and precision (N = 6) were below 5.0 %. The correlation coefficients of linear (R) for arsenic and mercury were both above 0.998. The limits of quantification (LOQ) were below 0.1 μg/L. The contents of As and Hg were defined in the dried citrus leaves and very near to the standard values provided by the manufacturer. The established method was applied to the analysis of three batches of Compound Niuhuang Xiaoyan capsule produced by three different manufacturers successfully. Insufficient realgar or cinnabar was formulated in some Compound Niuhuang Xiaoyan capsule samples.

Conclusion: :
The analysis showed that the contents of soluble arsenic and soluble mercury in artificial gastric juice were significantly less than that of total arsenic and total mercury in Compound Niuhuang Xiaoyan capsule. The results provided a reference for further study on the toxicology and pharmacokinetics of Compound Niuhuang Xiaoyan capsule.
Expression and Clinical Significance of Microtubule-Actin Cross-Linking Factor 1 in Serous Ovarian Cancer
Authors: Liu Longyang , Hu Ke , Zeng Zhaoyang , Xu Caiqiu , Lv Jin , Lin Zhongqiu , Wen Bin 
Published: February 2021

Background:
Ovarian Cancer (OC) remains the first leading cause of gynecologic malignancy.
The survival rate from Serous Ovarian Cancer (SOC) is very low, and the present prognostic
predictors of SOC are not very sensitive or specific.

Objective:
The present study aimed to investigate Microtubule-Actin Cross-Linking Factor 1 (MACF1) expression in SOC tissues (including paraffin-embedded and fresh tissues) and to assess its expression and significant value in patients with SOC.

Methods:
A total of 18 fresh SOC tissues and their paired paratumor tissues were performed with reverse-transcription quantitative PCR analysis to detect MACF1 mRNA expression. Moreover, 175 paraffin embedded SOC tissues and 41 paratumor tissues were assessed for MACF1 expression using immunohistochemistry.

Results:
The mRNA and protein expression of MACF1 were both higher in cancer tissues than that in paratumor tissues, and MACF1 high expression was associated with shorter Recurrence Free Survival (RFS) and Overall Survival (OS) in patients with SOC. Furthermore, multivariate regression analysis showed that high MACF1 expression was an independent poor survival predictor of patients with SOC.

Conclusion:
MACF1 is upregualted in SOC, and it may be used as a useful patent of prognostic biomarker in SOC.
The Endocannabinoid System and Alcohol Dependence: Will Cannabinoid
Receptor 2 Agonism be More Fruitful than Cannabinoid Receptor 1 Antagonism?
Authors: Oppong-Damoah Aboagyewaah , Gannon Brenda Marie , Murnane Kevin Sean 
Published: February 2021

:
Alcohol-use disorder (AUD) remains a major public health concern. In recent years,
there has been a heightened interest in components of the endocannabinoid system for the treatment
of AUD. Cannabinoid type 1 (CB1) receptors have been shown to modulate the rewarding effects
of alcohol, reduce the abuse-related effects of alcohol, improve cognition, exhibit anti-inflammatory,
and neuroprotective effects, which are all favorable properties of potential therapeutic candidates
for the treatment of AUD. However, CB1 agonists have not been investigated for the treatment
of AUD because they stimulate the motivational properties of alcohol, increase alcohol intake,
and have the tendency to be abused. Preclinical data suggest significant potential for the use
of CB1 antagonists to treat AUD; however, a clinical phase I/II trial with SR14716A (rimonabant),
a CB1 receptor antagonist/inverse agonist showed that it produced serious neuropsychiatric adverse
events such as anxiety, depression, and even suicidal ideation. This has redirected the field to
focus on alternative components of the endocannabinoid system, including cannabinoid type 2
(CB2) receptor agonists as a potential therapeutic target for AUD. CB2 receptor agonists are of particular
interest because they can modulate the reward pathway, reduce abuse-related effects of alcohol,
reverse neuroinflammation, improve cognition, and exhibit anti-inflammatory and neuroprotective
effects, without exhibiting the psychiatric side effects seen with CB1 antagonists. Accordingly,
this article presents an overview of the studies reported in the literature that have investigated CB2
receptor agonists with regards to AUD and provides commentary as to whether this receptor is a
worthy target for continued investigation.
Application of Spray-dried Microcapsules of Purslane (Portulaca oleracea L.) Seed Oil Enhances Quality of Mango Juice
Authors: Abozed Safaa S. , Elaraby Ghada M. , Zahran Hamdy A. 
Published: February 2021

Introduction:
Purslane (Portulaca oleracea L.) seeds oil are a non-traditional alpha-linolenic acid source (ALA), which is an omega-3 fatty acid. This study aimed to evaluate the physicochemical and sensory properties of mango juice fortified with purslane seed oil (PSO) microcapsules.

Materials and Methods:
Gum Arabic (GA) and maltodextrin, as wall-materials, were used in the microencapsulation of PSO by spray drying technique. The spray-dried microcapsules were added to the mango juice (200 mL) at the levels of 0.5, 1 and 1.5 g, ALA. Physicochemical properties such as viscosity, total soluble solids (TSS), pH and titratable acidity were measured, as well as sensory evaluation, during 28 days' storage at 4.0 ±0.5°C.

Results:
Our study indicated that the microencapsulation of PSO by spray drying resulted in the best microencapsulation yield (85.17%) as well as the microencapsulation efficiency (77.40%). The pH and TSS of four juice samples ranged from 3.0 to 3.6 and from 18.8 to 19.1 Brix°, respectively. In addition to that, storage periods had no significant effect on them.

Conclusion:
According to the findings presented in this paper, it has been concluded that the nutritional value of mango juices was enhanced by the addition of microencapsulated PSO as a source of ω-3 fatty acids.