bluuebunny/crossref_metadata_2025_split huggingface dataset:
Metabolic Engineering of Corynebacterium glutamicum for High‐Level Production of 1,5‐Pentanediol, a C5 Diol Platform Chemical
Authors: Sohn Yu Jung , Hwang Se‐Yeun , Lee Haeyoung , Jeon Subeen , Park Ji Young , Kim Jaehyung , Kim Donghyuk , Jeong Ki Jun , Lee Sang Yup , Joo Jeong Chan , Park Jin‐Byung , Park Si Jae 
Published: December 2024

AbstractThe biobased production of chemicals is essential for advancing a sustainable chemical industry. 1,5‐Pentanediol (1,5‐PDO), a five‐carbon diol with considerable industrial relevance, has shown limited microbial production efficiency until now. This study presents the development and optimization of a microbial system to produce 1,5‐PDO from glucose in Corynebacterium glutamicum via the l‐lysine‐derived pathway. Engineering began with creating a strain capable of producing 5‐hydroxyvaleric acid (5‐HV), a key precursor to 1,5‐PDO, by incorporating enzymes from Pseudomonas putida (DavB, DavA, and DavT) and Escherichia coli (YahK). Two conversion pathways for further converting 5‐HV to 1,5‐PDO are evaluated, with the CoA‐independent pathway—utilizing Mycobacterium marinum carboxylic acid reductase (CAR) and E. coli YqhD—proving greater efficiency. Further optimization continues with chromosomal integration of the 5‐HV module, increasing 1,5‐PDO production to 5.48 g L−1. An additional screening of 13 CARs identifies Mycobacterium avium K‐10 (MAP1040) as the most effective, and its engineered M296E mutant further increases production to 23.5 g L−1. A deep‐learning analysis reveals that Gluconobacter oxydans GOX1801 resolves the limitations of NADPH, allowing the final strain to produce 43.4 g L−1 1,5‐PDO without 5‐HV accumulation in fed‐batch fermentation. This study demonstrates systematic approaches to optimizing microbial biosynthesis, positioning C. glutamicum as a promising platform for sustainable 1,5‐PDO production.
Photocatalytic Oxidative Coupling of Methane to Ethane Using CO2 as a Soft Oxidant over the Au/TiO2−Vo Nanosheets
Authors: Wu Mingyu , Zhu Juncheng , Wu Yang , Liu Siying , Zheng Kai , Wang Shumin , Li Bangwang , Li Jing , Liu Chengyuan , Hu Jun , Zhu Junfa , Pan Yang , Sun Yongfu , Xie Yi 
Published: December 2024

AbstractPhotocatalytic oxidative coupling of methane (OCM) offers an appealing route for converting greenhouse gas into valuable C2 hydrocarbons. However, O2, as the most commonly used oxidant, tends to result in inevitable overoxidation and waste of methane feedstock. Herein, we first report a photocatalytic OCM using CO2 as a soft oxidant for C2H6 production under mild conditions, where an efficient photocatalyst with unique interface sites is designed and constructed to facilitate CO2 adsorption and activation, while concurrently boosting CH4 dissociation. As a prototype, the Au quantum dots anchored on oxygen‐deficient TiO2 nanosheets are fabricated, where the Au−Vo−Ti interface sites for CO2 adsorption and activation are collectively disclosed by in situ Kelvin probe force microscopy, quasi in situ X‐ray photoelectron spectroscopy and theoretical calculations. Compared with single metal site, the Au−Vo−Ti interface sites exhibit the lower CO2 adsorption energy and decrease the energy barrier of the *CO2 hydrogenation step from 1.05 to 0.77 eV via Au−C and Ti−O dual‐site bonding. The adsorbed CO2 on the photocatalyst reduces the energy barrier of *CH4 dissociation to *CH3 from 2.13 to 1.59 eV, contributing to CH4 oxidation. Additionally, in situ Fourier‐transform infrared spectroscopy unveils the Au site facilitates ethane production by engaging in *CH3−Au interaction and accelerating CH3−CH3 coupling. Thus, the photocatalyst demonstrates a high C2H6 evolution rate of 2.60 mmol g−1 h−1 for OCM using CO2 as the soft oxidant, surpassing most of previously reported photocatalysts regardless of OCM and nonoxidative coupling of methane. This work highlights the importance of soft oxidants for improving oxidation reaction efficiency and provides atomic scale insight into the design of photocatalysts for CH4 conversion.
Manipulating Interphase Chemistry for Aqueous Zn Stabilization: The Role of Supersaturation
Authors: Wang Qinyang , Zhang Zhaoyu , Hu Zuyang , Du Wencheng , Zhang Yufei , Ye Minghui , Wen Zhipeng , Tang Yongchao , Liu Xiaoqing , Li Cheng Chao 
Published: December 2024

AbstractThe limited cycling durability of Zn anode, attributed to the absence of a robust electrolyte‐derived solid electrolyte interphase (SEI), remains the bottleneck for the practical deployment of aqueous zinc batteries. Herein, we highlight the role of local supersaturation in governing the fundamental crystallization chemistry of Zn4SO4(OH)6⋅xH2O (ZSH) and propose a subtle supersaturation‐controlled morphology strategy to tailor the interphase chemistry of Zn anode. By judiciously creating local high‐supersaturation environment with organic caprolactam to manipulate the precipitation manner of zinc sulfate hydroxide (ZSH), lattice‐lattice matched heterogeneous nucleation of ZSH (001) and Zn (002) is realized in aqueous ZnSO4, producing a dense, pseudo‐coincidence interface capable of functioning as decent SEI. The plating/stripping efficacy of Zn is significantly boosted, as reflected by the great improvement of the initial (from 59.9 % to 84.1 %) and average (from 94.0 % to 99.2 %) coulombic efficiency. Accordingly, the cyclic endurance of Zn anode is prolonged from 50 h to over 7000 h at 0.5 mA cm−2/1 mAh cm−2, witnessing 140‐fold lifespan extension. Profiting from supersaturation‐mediated Zn stabilization, the performance deterioration of zinc‐vanadium batteries is also alleviated, even with a low N/P ratio of 4.4. This work is anticipated to deepen current understanding on the control of the anodic chemistry.
Fabrication of immobilized lipases for medium and long‐chain triacylglycerols preparation through solvent‐free acidolysis
Authors: Lin Shuping , Zeng Can , Zhang Qiangyue , Zhong Nanjing 
Published: December 2024

AbstractThe present study aims to fabricate efficient immobilized lipases for medium and long‐chain triacylglycerols preparation through acidolysis. Eighteen novel materials, including five mesoporous materials, four organic groups modified SBA‐15 samples (R‐SBA‐15) and nine macroporous resins, were employed as matrices for lipases immobilization. Lipases of TLL, AOL, RML and CALB were evaluated. Results indicated that TLL exhibited better acidolysis performance and decanoic acid content from 30% to 45% could be obtained; while other lipases (CALB, RML, and AOL) showed poor performance. In addition, macroporous resins of DA‐201, ADS‐17, D3520, and mesoporous silicas of SBA‐15, Al‐MCM‐41, HMS, were suitable for TLL immobilization. TLL@SBA‐15 was selected for acidolysis and the reaction conditions were optimized through single factors study and orthogonal design. The optimum conditions for the acidolysis were: lipase amount 6 wt%, decanoic acid: camellia oil molar ratio 14:1, reaction time 12 h and temperature 50°C. Under these conditions, decanoic acid content at 59.87%, oleic acid content 33.99% and sn‐2 decanoic acid content 7.41% was obtained (the mentioned fatty acids here were the fatty acids combined to oil molecules, not free fatty acids).
Piperazine‐based P2X4 receptor antagonists
Authors: Erlitz Katharina Sophie , Siutkina Alena I. , Prinz Ann‐Kathrin , Koch Oliver , Kalinin Dmitrii V. , Junker Anna 
Published: December 2024

AbstractThe P2X4 receptor (P2X4R), a ligand‐gated ion channel activated by ATP, plays a critical role in neuroinflammation, chronic pain, and cancer progression, making it a promising therapeutic target. In this study, we explored the design and synthesis of piperazine‐based P2X4R antagonists, building on the structural framework of paroxetine. A series of over 35 compounds were synthesized to investigate structure–activity relationships (SARs) in a Ca²⁺‐flux assay for P2X4R antagonistic activity. Several compounds outperformed paroxetine in terms of antagonistic P2X4R potency. Further studies on absorption, distribution, metabolism, excretion properties revealed that increased lipophilicity often correlated with high plasma protein binding and decreased metabolic stability, particularly in compounds with a naphthalene‐2‐yloxy group. Although promising SARs were observed, further optimization is needed to enhance antagonistic P2X4R receptor activity. This work provides important insights into the development of piperazine‐based P2X4R antagonists and lays the foundation for future therapeutic advancements targeting P2X4R‐related diseases.
The current landscape of 1,2,3‐triazole‐(fused) six‐membered nitrogen‐containing heteroaromatic ring hybrids with anticancer therapeutic potential
Authors: Xu Zhi , Li Rongqiang , Huang Zhiwei , Zhuang Yafei 
Published: December 2024

AbstractCancer, characterized by uncontrolled growth and spread of abnormal cells potentially influencing almost all tissues in the body, is one of the most devastating and lethal diseases throughout the world. Chemotherapy is one of the principal approaches for cancer treatment, but multidrug resistance and severe side effects represent the main barriers to the success of therapy, creating a vital need to develop novel chemotherapeutic agents. The 1,2,3‐triazole moiety can be conveniently constructed by “click chemistry” and could exert diverse noncovalent interactions with various enzymes in cancer cells. Hence, 1,2,3‐triazole is one of the most fascinating anticancer pharmacophores. Moreover, 1,2,3‐triazole could also serve as a powerful ligation tool for the complex molecular architectures to increase the anticancer efficacy of lead molecules. Notably, 1,2,3‐triazole‐containing hybrids with intriguing structural variations could target different biological components in cancer cells simultaneously, highlighting their potential in the treatment and eradication of cancer. This review outlines the current landscape of 1,2,3‐triazole‐(fused) six‐membered nitrogen‐containing heteroaromatic ring hybrids, inclusive of 1,2,3‐triazole‐quinazolines, 1,2,3‐triazole‐quinazolinones, 1,2,3‐triazole‐quinolines, 1,2,3‐triazole‐quinolones, 1,2,3‐triazole‐pyridines, and 1,2,3‐triazole‐pyrimidines, with anticancer therapeutic potential, and explores their mechanisms of action, critical aspects of design as well as structure–activity relationships (SARs), covering articles published from 2021 to the present, to pave the way for the development of innovative and efficient therapeutic interventions for cancer therapy.
The use of bibliometrics for ranking the all‐time greatest music artists
Authors: Urban Timothy L. 
Published: December 2024

AbstractThis brief communication presents a novel adaptation of common bibliometric measures to provide a quantitative assessment of an artist's music catalog that incorporates both impact and productivity. Data from Billboard's weekly Hot 100™ music charts are used to rank the all‐time greatest artists. Since the sorted data are increasing in value—that is, a number 1 hit is best—a transformation is applied to provide a convex, monotonically decreasing curve. Furthermore, since conventional bibliometrics result in several artists with identical measures, metrics inspired by the multidimensional ‐ and ‐indices are used to rank the artists. We find that this approach provides a simple, yet unbiased, approach for ranking the all‐time greatest music artists.
A Novel, Site‐Specific N‐Linked Glycosylation Model Provides Mechanistic Insights Into the Process‐Condition Dependent Distinct Fab and Fc Glycosylation of an IgG1 Monoclonal Antibody Produced by CHO VRC01 Cells
Authors: Reddy Jayanth Venkatarama , Leibiger Thomas , Singh Sumit Kumar , Lee Kelvin H. , Papoutsakis Eleftherios , Ierapetritou Marianthi 
Published: December 2024

ABSTRACTThe CHO VRC01 cell line produces an anti‐HIV IgG1 monoclonal antibody containing N‐linked glycans on both the Fab (variable) and Fc (constant) regions. Site‐specific glycan analysis was used to measure the complex effects of cell culture process conditions on Fab and Fc glycosylation. Experimental data revealed major differences in glycan fractions across the two sites. Bioreactor pH was found to influence fucosylation, galactosylation, and sialylation in the Fab region and galactosylation in the Fc region. To understand the complex effects of process conditions on site‐specific N‐linked glycosylation, a kinetic model of site‐specific N‐linked glycosylation was developed. The model parameters provided mechanistic insights into the differences in glycan fractions observed in the Fc and Fab regions. Enzyme activities calculated from the model provided insights into the effect of bioreactor pH on site‐specific N‐linked glycosylation. Model predictions were experimentally tested by measuring glycosyltransferase‐enzyme mRNA‐levels and intracellular nucleotide sugar concentrations. The model was used to demonstrate the effect of increasing galactosyltransferase activity on site‐specific N‐linked glycan fractions. Experiments involving galactose and MnCl2 supplementation were used to test model predictions. The model is capable of providing insights into experimentally measured data and also of making predictions that can be used to design media supplementation strategies.
Biocompatibility of Novel Marine Collagen on Periodontal Ligament Fibroblasts: A Pathway to Enhanced Regenerative Therapies
Authors: Siddiqui Yasir Dilshad , Arief Erry Mochamad , Saddique Muhammad Aliyan 
Published: December 2024

ABSTRACTBody wall of sea cucumber Bohadschia bivittate contains a protein consisting of highly insoluble collagen fibers. We aimed to evaluate the biocompatibility and cytotoxicity of nonirradiated or γ‐irradiated pepsin soluble collagen (PSC) extracted from Bohadschia bivittata on human periodontal ligament fibroblasts cells. The MTT assay showed significant increase in the cell viability values indicating that PSC is noncytotoxic. Further, nonirradiated PSC showed higher cell viabilities values than γ‐irradiated PSC at all concentration, especially at an optimal dilution of 25% (p < 0.05). This exploratory study suggests that PSC from Bohadschia bivittata could be utilized as a novel biomaterial in periodontal regenerative therapies.
Acquired Lymphangioma Circumscriptum of Vulva in a Patient With Hidradenitis Suppurativa: A Rare Case at a Rare Site
Authors: Pokharel Ranju , Marahatta Suchana 
Published: December 2024

ABSTRACTLymphangioma circumscriptum, one of the common forms of lymphangioma, can be either congenital or acquired. Various acquired conditions can lead to its causation and one of the causes might be untreated hidradenitis suppurativa. So, this report highlights the need of early interventions for hidradenitis suppurativa as lymphangioma circumscriptum can be one of its consequences later in life. It can occur in various sites, often around neck, axilla, and groin. Post‐surgery and radiotherapy, tuberculosis, Crohn's disease are well known cause to develop acquired vulval lymphangioma. Here, we present a case of a 20 years female with acquired vulval lymphangioma secondary to hidradenitis suppurativa which is one of the rare cause for vulval lymphangioma.
Live Births After Laparoscopic Wedge Resection for Unruptured Ovarian Pregnancy With Hemorrhagic Hypotension: A Case Report
Authors: Hirahara Yuhya , Nagai Koichi , Mukaida Kazunori 
Published: December 2024

ABSTRACTPatients with an unruptured ovarian pregnancy may exhibit signs of shock. Laparoscopy is well‐suited for the detection of ovarian pregnancy. Viable pregnancies are possible for patients with ovarian pregnancy and a low risk of ectopic pregnancy.
The Complex Task of Evaluating the Institutional Adaptive Capacity to Climate Change at Local Government Level: A Study of the Eastern Cape Province of South Africa
Authors: Gadu Siyaxola Ernest , Adom Richard Kwame , Simatele Mulala Danny 
Published: December 2024

ABSTRACTClimate change impacts are wreaking havoc in South Africa, particularly in the Eastern Cape Province. Hence, adaptation strategies are essential tools in the Eastern Cape, as the province is among the most vulnerable regions to climate change impacts in South Africa. In response and to minimise the adverse impacts of climate change on socioeconomic factors and livelihoods, the post‐apartheid South African government developed a policy framework for climate governance, with a focus on policy planning and adaptation. Although the policy sounds good and solution‐driven, the implementation of the programme at local levels to achieve the desired goals remained a significant challenge due to institutional and capacity challenges. Using research methods inspired by the tradition of qualitative and quantitative research approach and existing literature, this article explored the complexities of evaluating and monitoring the adaptive capacity to climate change governance at a local government level in South Africa, focusing on the Eastern Cape Province. The findings of this article uncovered that the institutional and capacity challenges create an unconducive environment for an evaluation of institutional adaptive capacity to climate change at local government level in South Africa. The absence of a reliable system to assess the institutional adaptive capacity to climate change at local government level makes it difficult to compare the adaptive capacity of different institutions and allocate available resources in an adequate manner. The article recommends a broader discourse of the sustainable development goals, particularly goal number 13, which encourages the strengthening of resilience and adaptive capacity to climate change impacts.
Short‐Term Urinary Incontinence After Radical Prostatectomy Is Still Based on Patients' Age, Nerve‐Sparing Approach, and Surgical‐Experience, Despite the Higher‐Use of Robotic Surgery in 2022 Compared to 2016 Real‐World Results of a Large Rehabilitation Center in Germany
Authors: Püllen Lukas , Naumann Max , Krafft Ulrich , Püllen Felix , Mahmoud Osama , Al‐Nader Mulham , Darr Christopher , Borgmann Hendrik , Briel Christoph , Hadaschik Boris , Salem Johannes , Kuru Timur 
Published: December 2024

ABSTRACTBackgroundDespite constant improvements, incontinence is one of the most relevant and quality‐of‐life‐reducing side effects of radical prostatectomy (RP) and, in addition to patient‐specific factors such as age, the experience of the surgeon/center and the surgical technique used play an important role.AimsTo present current real‐world data on short‐term incontinence after RP from one of the largest German rehabilitation centers in 2022 and to compare it to the results from the same institution in 2016.Methods and ResultsRetrospective, unicentric, univariate analysis of data from 1394 men after RP in 2022 on admission and discharge from the rehabilitation clinic. Incontinence defined as ≥ 1 pad/day was evaluated by quantitative measuring all day incontinence under a defined graduation and compared to the results of 2016. Totally, 1393 men were available for analysis in 2022 compared to 1390 in 2016. Median age for both cohorts was 66 years with minor differences in preoperative PSA levels. Despite different surgical approaches, no significant change in short‐term incontinence rates in 2016 and 2022 were noted at discharge (76.9% vs. 77.9%, p = 0.56). A notable increase in patients with ISUP grade Group 2 and a shift towards robotic surgery were observed in 2022 (45.5%–71%). While nerve sparing led to a significant improvement in continence (p < 0.01), lymphadenectomy and T‐stage were not related to any significant increase in short‐term incontinence rates. Comparing age groups within the cohort, patients > 69 years exhibited the highest risk of short‐term incontinence and least likelihood of regaining continence during rehabilitation (p < 0.01). Men treated at a certified prostate cancer center had significantly (p < 0.01) lower short‐term incontinence rates.ConclusionOur study shows little improvement in short‐term postoperative incontinence rates after RP in Germany in the last 6 years and known risk factors for postoperative incontinence like age, nerve‐sparing surgery, and level of experience were reproduced in our analyses. We conclude not only to carefully select but also to counsel patients before being treated for prostate cancer and to strongly advice treatment at certified centers.
Annual Banned‐Substance Review 17th Edition—Analytical Approaches in Human Sports Drug Testing 2023/2024
Authors: Thevis Mario  , Kuuranne Tiia  , Geyer Hans  
Published: December 2024

ABSTRACTThe 17th edition of the annual banned‐substance review on analytical approaches in human sports drug testing is dedicated to literature published between October 2023 and September 2024. As in previous years, focus is put particularly on new or enhanced analytical options in human doping controls as well as investigations into the metabolism and elimination of compounds of interest, which represent central (while not exclusive) cornerstones of the global anti‐doping mission. New information published within the past 12 months on established doping agents as well as new potentially relevant substances are reviewed and discussed in the context of the World Anti‐Doping Agency's 2024 Prohibited List. Thereby, analytical challenges, especially with regard to the continuously growing number of target compounds and potentially relevant drug classes as well as the exigency (and consequences) of utmost analytical retrospectivity, are thematized and contextualized. Investigations especially into anabolic agents, peptide hormones, and strategies for the detection of gene doping were identified as core areas of anti‐doping research in the reviewed period.
Prognostic role of high‐sensitivity cardiac troponin T in patients with cardiac sarcoidosis: insights from ILLUMINATE‐CS
Authors: Baba Yuichi , Kubo Toru , Nabeta Takeru , Matsue Yuya , Kitai Takeshi , Naruse Yoshihisa , Taniguchi Tatsunori , Tanaka Hidekazu , Okumura Takahiro , Yoshioka Kenji , Kitaoka Hiroaki 
Published: December 2024

AbstractAimsThe prognostic role of high‐sensitivity cardiac troponin T (hs‐cTnT) as a biomarker in patients with cardiac sarcoidosis (CS) has yet to be fully determined, especially when compared with B‐type natriuretic peptide (BNP).Methods and ResultsIn this post‐hoc analysis of the ILLUMINATE‐CS (ILLUstration of the Management and prognosIs of JapaNese pATiEnts with Cardiac Sarcoidosis), which is a multicentre retrospective observational study, we analysed 103 patients (62.2 ± 10.9 years old, 31.1% male) diagnosed as CS and with available data for hs‐cTnT measured at the time of diagnosis. The primary outcome was the combined outcomes of all‐cause death, fatal ventricular arrhythmia events and heart failure hospitalization. During a median follow‐up period of 2.6 (inter‐quartile range, 1.6–5.7) years, 24 primary outcomes were observed. Patients with a high hs‐cTnT level, defined as a level above the median value (>0.016 ng/mL), were associated with a higher incidence of adverse events than those with a low hs‐cTnT level (log‐rank, P = 0.017). In Cox regression analysis, a high log‐transformed hs‐cTnT level and a high log‐transformed BNP level were significant risk factors for primary outcome [hazard ratio (HR), 4.368 (95% confidence interval, CI, 1.032–18.480), P = 0.045. and HR, 3.127 (95% CI, 1.029–9.499), P = 0.044, respectively]. Patients with both high hs‐cTnT and high BNP (>140 pg/mL: above the median value) levels had a 3.49 (95% CI, 1.23–9.88)‐fold increased risk of the primary outcome compared with patients with both low hs‐cTnT and low BNP levels.ConclusionsIn patients with CS, a high hs‐cTnT level is a useful predictor of adverse events, and combined measurement of hs‐cTnT and BNP further improves the prognostic value.