bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effect of different types of anthropogenic pollution on the bacterial community of urban rivers
Authors: Zhang Lei , Cheng Yu , Zhou Yi , Lu Wenxuan , Li Jing 
Published: January 2021

AbstractThe health of urban rivers is threatened by multiple anthropogenic stressors. Bacterial communities in rivers can quickly respond to different types of polluted environments, making them useful for water quality assessments and predictive insights. However, research on river bacterial communities has largely ignored interactions between these communities. Here, 16S rRNA amplicon sequencing analysis is used to comprehensively analyze the bacterial communities in the water and sediments in different types of anthropogenically impacted urban river. The results show that distinct differences occur in the bacterial communities in the river sediment and water with different pollution types. The changes in the bacterial communities in sediments were more pronounced than those in the water. A modular analysis further showed that the microbial co‐occurrence network under different types of pollution had a nonrandom modular structure, and this structure was mainly driven by classification correlation and bacterial function. Genes identified for nitrogen cycling in all the river water and sediment samples included major functional genes for nitrogen fixation, assimilatory nitrogen reduction, nitrification, denitrification, and ammonification. Carbon degradation genes were mainly observed in the carbon cycle. Taken together, the above findings provide further insights into microbial communities in urban river ecosystems under anthropogenic contamination.Practitioner points

The physical and chemical indicators of the four types of pollution drive bacterial community structure.
Bacterial community has C, N, P metabolic genes indicating its ecological effect.
River bacteria were connected more frequently in the same or similar type of pollution in the co‐occurrence network.
Microbe–environment correlations and microbe–microbe interactions were combined to determine crucial indicators.
Union rates and functional outcome of double plating of the femur: systematic review of the literature
Authors: Lodde M. F. , Raschke M. J. , Stolberg-Stolberg J. , Everding J. , Rosslenbroich S. , Katthagen J. C. 
Published: January 2021

AbstractBackgroundThe optimal treatment strategy for the surgical management of femur fractures and non-unions remains unknown. The aim of this study is to assess union rates, complications and outcome after femoral double plating. Treatment of shaft, distal, periprosthetic fractures and pathological proximal femur fractures as well as femoral non-unions with double plating were evaluated.MethodsA systematic review according to the Preferred Reporting Items for Systematic reviews and Meta-Analyses (PRISMA) statement was conducted. Published literature reporting on the treatment and clinical outcome of femoral fractures and non-unions with double plating was identified. In total, 24 studies with 436 cases of double plating, 64 cases of single plating, 84 cases of intramedullary nailing (IM), and 1 interfragmentary screw treatment met the inclusion criteria of this systematic review. The evaluated literature was published between 1991 and 2020.ResultsDouble plating of femoral fractures achieved high healing rates and few complications were reported. It displayed significantly less intraoperative haemorrhage, shorter surgery time reduced risk of malunion in polytraumatised patients when compared to IM. Fracture healing rate of double-plating distal femoral fractures was 88.0%. However, there were no significant differences regarding fracture healing, complication or functional outcome when compared to single plating. Treatment of periprosthetic fractures with double plating displayed high healing rates (88.5%). Double plating of non-unions achieved excellent osseous union rates (98.5%).ConclusionsThe literature provides evidence for superior outcomes when using double plating in distal femoral fractures, periprosthetic fractures and femoral non-unions. Some evidence suggests that the use of double plating of femoral fractures in polytraumatised patients may be beneficial over other types of fracture fixation.Level of evidenceIV.
Robust and Fast UV–HPLC Method for Biotransformation Analysis of Azecines
Authors: Zergiebel Stephanie , Seeling Andreas 
Published: January 2021

AbstractDibenzoazecines are a new class of drug candidates for the treatment of schizophrenia. Compared to the drugs currently used in therapy, the azecines have a novel mechanism of action. Thus, they have the potential to cause fewer side effects compared to the standard therapy with a constant high neuroleptic potency. This theory was substantiated by comparative in vivo tests with haloperidol and risperidone. Seventeen new azecine derivatives have already been tested furthermore of stability, physicochemical parameters, pharmacokinetics including esterase cleavage, stability in simulated gastrointestinal fluid, stability at different pH values and determination of octanol/water-partition coefficients. For these substances, class is still a lack of information concerning the metabolism. Therefore, the present study investigated and developed a reliable and reproducible gradient reversed-phase HPLC–UV method to determinate the lead structure LE404 alongside emerging metabolites in compliance with international requirements like ICH guidelines and the European Pharmacopoeia. Up to now, there is no innovative method suitable for such a determination. Chromatographic separations were achieved with a phenomenex™ Gemini column (5 µm C18 110 Å, 250 × 4.60 mm) using a mixture of acetonitrile/potassium dihydrogen phosphate buffer (4 mmol L−1, pH 2.5) as mobile phase. The gradient method flow rate was 1.0 mL min−1, and UV detection was made at 220 nm. The optimized HPLC method was found to be specific, accurate, reproducible and robust for determination of LE404.
A novel lymphatic pattern promotes metastasis of cervical cancer in a hypoxic tumour-associated macrophage-dependent manner
Authors: Chen Xiao-Jing , Wei Wen-Fei , Wang Zi-Ci , Wang Nisha , Guo Chu-Hong , Zhou Chen-Fei , Liang Luo-Jiao , Wu Sha , Liang Li , Wang Wei 
Published: January 2021

AbstractLymphatic remodelling in the hypoxic tumour microenvironment (TME) is critically involved in the metastasis of cervical squamous cell carcinoma (CSCC); however, its underlying mechanisms remain unclear. Here, we uncovered a novel lymphatic pattern in the hypoxic TME, wherein lymphatic vessels (LVs) are encapsulated by tumour-associated macrophages (TAMs) to form an interconnected network. We describe these aggregates as LVEM (LVs encapsulated by TAMs) considering their advantageous metastatic capacity and active involvement in early lymph node metastasis (LNM). Mechanistic investigations revealed that interleukin-10 (IL-10) derived from hypoxic TAMs adjacent to LVs was a prerequisite for lymphangiogenesis and LVEM formation through its induction of Sp1 upregulation in lymphatic endothelial cells (LECs). Interestingly, Sp1high LECs promoted the transactivation of C–C motif chemokine ligand 1 (CCL1) to facilitate TAM and tumour cell recruitment, thereby forming a positive feedback loop to strengthen the LVEM formation. Knockdown of Sp1 or blockage of CCL1 abrogated LVEM and consequently attenuated LNM. Notably, CSCCnon-LNM is largely devoid of hypoxic TAMs and the resultant LVEM, which might explain its metastatic delay. These findings identify a novel and efficient metastasis-promoting lymphatic pattern in the hypoxic TME, which might provide new targets for anti-metastasis therapy and prognostic assessment.
Chronic kidney disease impairs prognosis in electrical storm
Authors: Weidner Kathrin , Behnes Michael , Schupp Tobias , Hoppner Jorge , Ansari Uzair , Mueller Julian , Lindner Simon , Borggrefe Martin , Kim Seung-hyun , Huseyinov Aydin , Ellguth Dominik , Akin Muharrem , Meininghaus Dirk Große , Bertsch Thomas , Taton Gabriel , Bollow Armin , Reichelt Thomas , Engelke Niko , Reiser Linda , Akin Ibrahim 
Published: January 2021

Abstract
Background
The study sought to assess the prognostic impact of chronic kidney disease (CKD) in patients with electrical storm (ES). ES represents a life-threatening heart rhythm disorder. In particular, CKD patients are at risk of suffering from ES. However, data regarding the prognostic impact of CKD on long-term mortality in ES patients is limited.

Methods
All consecutive ES patients with an implantable cardioverter–defibrillator (ICD) were included retrospectively from 2002 to 2016. Patients with CKD (MDRD-GFR < 60 ml/min/1.73 m2) were compared to patients without CKD. The primary endpoint was all-cause mortality at 3 years. Secondary endpoints were in-hospital mortality, cardiac rehospitalization, recurrences of electrical storm (ES-R), and major adverse cardiac events (MACE) at 3 years.

Results
A total of 70 consecutive ES patients were included. CKD was present in 43% of ES patients with a median glomerular filtration rate (GFR) of 43.3 ml/min/1.73 m2. CKD was associated with increased all-cause mortality at 3 years (63% vs. 20%; p = 0.001; HR = 4.293; 95% CI 1.874–9.836; p = 0.001) and MACE (57% vs. 30%; p = 0.025; HR = 3.597; 95% CI 1.679–7.708; p = 0.001). In contrast, first cardiac rehospitalization (43% vs. 45%; log-rank p = 0.889) and ES-R (30% vs. 20%; log-rank p = 0.334) were not affected by CKD. Even after multivariable adjustment, CKD was still associated with increased long-term mortality (HR = 2.397; 95% CI 1.012–5.697; p = 0.047), as well as with the secondary endpoint MACE (HR = 2.520; 95% CI 1.109–5.727; p = 0.027).

Conclusions
In patients with ES, the presence of CKD was associated with increased long-term mortality and MACE.
The ideological use and abuse of Freiburg’s ordoliberalism
Authors: Dold Malte , Krieger Tim 
Published: January 2021

AbstractIn the aftermath of the Eurozone crisis, a battle of ideas emerged over whether ordoliberalism is part of the cause or the solution of economic problems in Europe. While German ordoliberals argued that their policy proposals were largely ignored before, during and after the crisis, critics saw too much ordoliberal influence, especially in form of austerity policies. We argue that neither view is entirely correct. Instead, we observe that the battle of ideas is largely independent of the countries’ actual responses to the Eurozone crisis: pragmatic self-interest on behalf of governments rather than their ideological convictions played a crucial role in political reactions. We explain this dynamic game-theoretically and highlight a number of reasons for the decoupling of the political-pragmatic debate from the ideological-academic discourse. In addition, we argue that ordoliberals themselves contributed to the ideological misuse of their own program: the ordoliberal Freiburg School ceased to be an active research program and instead grew to resemble a tradition which all too often disregarded the international academic discourse, in particular in macroeconomics. As a result, ordoliberal thinking was abused by its proponents and critics alike to emphasize their preconceived Weltanschauung (worldview). We end our paper with some thoughts on how a contemporary ordoliberalism can be constructively used to react to some of the challenges of the ongoing Eurozone crisis.
Microplastic abundance in beach sediments of the Kiel Fjord, Western Baltic Sea
Authors: Schröder Kevin , Kossel Elke , Lenz Mark 
Published: January 2021

AbstractWe assessed the abundance of microplastics (0.2–5 mm) in drift line sediments from three sites in Kiel Fjord, Western Baltic Sea. The first site is intensively used by beach visitors, the second is in close proximity to a sewage plant and the third is polluted with large-sized plastic litter. Samples were split into three grain size classes (0.2–0.5, 0.5–1, 1–5 mm), washed with calcium chloride solution, and filtered at 0.2 mm. Filters were then visually inspected, and a total of 180 fragments was classified as microplastics, of which 39% were analyzed using Raman spectroscopy. At the site that is close to a sewage plant as well as at the site with intense beach use, 1.8 and 4.5 particles (fibers plus fragments) per kg of dry sediment were found, respectively, while particle abundances reached 30.2 per kg of dry sediment at the site with high litter loads. Our data suggest that the fragmentation of large plastic debris at site seems to be a relevant source for microplastics in Western Baltic Sea beach sediments.
In Defense of the Lenient View
Authors: Schaber Peter 
Published: January 2021

AbstractThis paper deals with the wrongness of having sex with someone without her valid consent. There are good reasons to think that deception about deal-breakers invalidate consent to sex and that acting without valid consent wrongs the consenter. Tom Dougherty argues that it is always seriously wrong to deceive another person into sex by deceiving her. We should on his view therefore reject the view that doing so is in certain cases only a minor wrong (this is what he calls “the lenient view”). It will be argued here that we should accept this view. I will argue against Dougherty that the lenient view does not presuppose an unacceptably moralistic view of sexuality. In addition, the lenient view can be defended by what I call the harm view of the wrongness of having sex without valid consent. If no or only mild harm is caused deceiving someone into sex is only a minor wrong.
A Survey of Traffic Prediction: from Spatio-Temporal Data to Intelligent Transportation
Authors: Yuan Haitao , Li Guoliang 
Published: January 2021

AbstractIntelligent transportation (e.g., intelligent traffic light) makes our travel more convenient and efficient. With the development of mobile Internet and position technologies, it is reasonable to collect spatio-temporal data and then leverage these data to achieve the goal of intelligent transportation, and here, traffic prediction plays an important role. In this paper, we provide a comprehensive survey on traffic prediction, which is from the spatio-temporal data layer to the intelligent transportation application layer. At first, we split the whole research scope into four parts from bottom to up, where the four parts are, respectively, spatio-temporal data, preprocessing, traffic prediction and traffic application. Later, we review existing work on the four parts. First, we summarize traffic data into five types according to their difference on spatial and temporal dimensions. Second, we focus on four significant data preprocessing techniques: map-matching, data cleaning, data storage and data compression. Third, we focus on three kinds of traffic prediction problems (i.e., classification, generation and estimation/forecasting). In particular, we summarize the challenges and discuss how existing methods address these challenges. Fourth, we list five typical traffic applications. Lastly, we provide emerging research challenges and opportunities. We believe that the survey can help the partitioners to understand existing traffic prediction problems and methods, which can further encourage them to solve their intelligent transportation applications.
Development of in-situ adaptive controller for end milling based on vibration feedback
Authors: Reddy L. Vamsi Krishna , Prasad Balla Srinivasa , Raghuram N. Harsha , Rajasekhar M. 
Published: January 2021

AbstractThis study establishes a tool condition monitoring methodology builds on the vibration signal attained via data acquisition system which is integrated with the in house developed adaptive controller for an end milling. As the quality of the products and the machine tool performance are the key parameters in maintaining machine stability. Proposed Adaptive control optimization system is validated with the experimentation trials and data analysis on 3 axis CNC milling machine. The rotational speed of the spindle and vibration signals is found to be reactive to milling cutter condition and therefore capable of sustaining the set-out methodology. A novel hybrid transformation, coupled with FFT and HHT is proposed to distinguish between a source of variation for adaptive control optimization, cutting region with the non-cutting region. In this study, decisions are made to evaluate the tool condition by combining all related information into a rule base. The investigation trajectories unveil the established system be able to accomplish the mechanism properly as anticipated.
The Rayleigh–Taylor instability in a porous medium
Authors: Forbes Lawrence K. , Browne Catherine A. , Walters Stephen J. 
Published: January 2021

AbstractThe classical Rayleigh–Taylor instability occurs when a heavy fluid overlies a lighter one, and the two fluids are separated by a horizontal interface. The configuration is unstable, and a small perturbation to the interface grows with time. Here, we consider such an arrangement for planar flow, but in a porous medium governed by Darcy’s law. First, the fully saturated situation is considered, where the two horizontal fluids are separated by a sharp interface. A classical linearized theory is reviewed, and the nonlinear model is solved numerically. It is shown that the solution is ultimately limited in time by the formation of a curvature singularity at the interface. A partially saturated Boussinesq theory is then presented, and its linearized approximation predicts a stable interface that merely diffuses. Nonlinear Boussinesq theory, however, allows the growth of drips and bubbles at the interface. These structures develop with no apparent overturning at their heads, unlike the corresponding flow for two free fluids.
Evaluation of nutritional characteristics and consumers’ acceptance of gluten-free sweet biscuits made from rice-based pregelatinized composite flours containing orange pomace and soy protein isolate
Authors: Cayres Caroline Alves , Ascheri José Luis Ramírez , Couto Maria Antonieta Peixoto Gimenes 
Published: January 2021

AbstractGluten-free bakery products are usually deficient in dietary fiber and inferior sources of protein. Studies that have assessed sensory characterization of products prepared with orange pomace are scarce. This study evaluated the employment of 30% of rice-based pregelatinized composite flours (containing 15, 25 or 35% orange pomace blended with 5% soy protein isolate) on gluten-free biscuits. The target was the investigation of the nutritional profile and consumer’s acceptance of three short dough sweet biscuit samples. Thermoplastic extrusion was an efficient unit operation for the elaboration of a new gluten-free ingredient in which a by-product rich in total dietary fiber and a component with a high level of protein were employed. All pregelatinized composite flours in this study can be labeled as protein sources and can be claimed as high in total dietary fiber content, as well as two biscuit samples that can be labeled as total dietary fiber source. Also, all pregelatinized composite flours and biscuits presented low moisture and water activity, being microbiologically stable to room temperature (25 ± 2 °C). Complementarily, the results of the microbiological evaluations [(coagulase-positiveStaphylococcus, thermotolerant coliform (45 °C) andSalmonellasp. (absence in 25 g)] of the biscuits and their comparison with the Brazilian microbiological standards legislation attested their safety. Concerning the sensory analysis, adults’ results showed that the flavor attribute might be responsible for increasing the overall impression score of a sample. In contrast, children’s results showed that they did not distinguish (p ≥ 0.05) two of three samples of biscuits analyzed.
Phosphatidylserine and phosphatidylethanolamine regulate the structure and function of FVIIa and its interaction with soluble tissue factor
Authors: Sengupta Tanusree , Koklic Tilen , Lentz Barry R. , Majumder Rinku 
Published: January 2021

Abstract
Cell membranes have important functions in many steps of the blood coagulation cascade, including the activation of factor X (FX) by the factor VIIa (FVIIa)-tissue factor (TF) complex (extrinsic Xase). FVIIa shares structural similarity with factor IXa (FIXa) and FXa. FIXa and FXa are regulated by binding to phosphatidylserine (PS)-containing membranes via their γ-carboxyglutamic acid-rich domain (Gla) and epidermal growth-factor (EGF) domains. Although FVIIa also has a Gla-rich region, its affinity for PS-containing membranes is much lower compared with that of FIXa and FXa. Research suggests that a more common endothelial cell lipid, phosphatidylethanolamine (PE), might augment the contribution of PS in FVIIa membrane-binding and proteolytic activity. We used soluble forms of PS and PE (1,2-dicaproyl-sn-glycero-3-phospho-l-serine (C6PS), 1,2-dicaproyl-sn-glycero-3-phospho-ethanolamine (C6PE)) to test the hypothesis that the two lipids bind to FVIIa jointly to promote FVIIa membrane binding and proteolytic activity. By equilibrium dialysis and tryptophan fluorescence, we found two sites on FVIIa that bound equally to C6PE and C6PS with Kd of ∼ 150–160 μM, however, deletion of Gla domain reduced the binding affinity. Binding of lipids occurred with greater affinity (Kd∼70–80 μM) when monitored by FVIIa proteolytic activity. Global fitting of all datasets indicated independent binding of two molecules of each lipid. The proteolytic activity of FVIIa increased by ∼50–100-fold in the presence of soluble TF (sTF) plus C6PS/C6PE. However, the proteolytic activity of Gla-deleted FVIIa in the presence of sTF was reduced drastically, suggesting the importance of Gla domain to maintain full proteolytic activity.
Therapieoptionen bei postoperativen Seromen
Authors: Wigbers Franziska M. , Woitalla-Bruning Jasmin 
Published: January 2021

ZusammenfassungPostoperative Serome können im chirurgischen Alltag immer wieder eine Herausforderung darstellen. Die Therapiemöglichkeiten sind vielfältig. Oftmals erfolgt zunächst die sterile Punktion mit anschließender Aspiration der Seromflüssigkeit und Anlage eines Druckverbandes. Eine alternative Therapiemöglichkeit bietet der zusätzliche Einsatz von Polidocanol oder Antibiotika, wie z. B. Erythromycin oder Doxycyclin. Bei besonders hartnäckigen, punktionsresistenten Seromen sollte eine perkutane Durchstichdrainage oder eine offene Revision als Therapiealternative in Betracht gezogen werden.