bluuebunny/crossref_metadata_2025_split huggingface dataset:
An Approach For The Reconstruction of a Traditional Masonry-Wooden Building Located In An Archeological Area. Part I: Methodology
Authors: Akçay Cemil , Korkmaz Nail Mahir , Sayin Baris 
Published: December 2021

Abstract
This study, presented in a pair of articles, defines a comprehensive methodological approach to the reconstruction of a traditional masonry-timber mansion building constructed in the 1880s of the Turkish house typology on Istanbul’s historical peninsula area that was intentionally demolished in 1948. A historical process research was carried out in the first stage of the study, after which ground penetration radar measurements and an archeological excavation were carried out to determine any possible remains or ruins of the structure, and the original architectural features of the demolished building were ascertained from the obtained data. The proposed stages in the current paper can be considered a comprehensive approach to the determination of the authentic properties of demolished or destroyed buildings in historical areas, given that the methodology allows for the integration of construction features obtained separately and independently through different activities, such as excavations, georadar measurements and historical surveys. The result is a versatile approach to the complete and realistic reconstruction of historical buildings.
Microstructural and Mechanical Aspects of AlSi7Mg0.6 Alloy Related to Scanning Strategies in L-PBF
Authors: Yadav Pinku , Rigo Olivier , Arvieu Corinne , Lacoste Eric 
Published: December 2021

Abstract
AlSi7Mg0.6 alloy is the most widely used cast alloy for aerospace and automotive applications. Therefore, it is essential to explore the effect of scanning strategies parameters on the final part properties in the L-PBF process. The effect of stripes and chessboard strategies parameters such as stripes length, rotation angle, and chessboard island size on mechanical and microstructural properties of L-PBF processed AlSi7Mg0.6 alloy is studied. The evolution of the residual stresses is also investigated in the longitudinal and transverse directions. Cooling rates are also estimated using the cell size within the melt pool. Three distinct regions (i.e., fine, coarse, and heat affected zone) within the melt pool corresponding to different cooling rates could be identified based on Si morphology. The texture of the final material can be tailored by changing the scanning strategies. This study comprehensively presents the results concerning porosity, mechanical properties, crystallographic texture, cooling rates, grain morphology, and residual stresses for additively manufactured AlSi7Mg0.6 alloy.
Mosquito Species Composition And Insecticide Resistance Status of Anopheles Arabiensis In Itang Special Woreda, Gambella, Southwestern Ethiopia
Authors: Chanyalew Tebiban , Natea Gadisa , Amenu Desalegn , Yewhalaw Delenasaw , Simma Eba Alemayehu 
Published: December 2021

Abstract
Introduction: Anopheles arabiensis, member species of the Anopheles gambiae complex, is the primary vector of malaria widely distributed in Ethiopia. Anopheles funestus, An. pharoensis and An. nili are secondary vectors occurring with limited distribution in the country. Indoor residual spraying (IRS) and long-lasting insecticidal nets (LLINs) are pillars for the interventions against malaria control and elimination efforts in Ethiopia. However, the emergence and widespread of insecticide resistance in the major malaria vector, An. arabiensis, might compromise the efforts of the country. The aim of this study was to investigate composition of mosquito species and insecticide resistance status of An. arabiensis in Itang special woreda (district), Gambella, southwestern Ethiopia.Materials and methods: Adult mosquitoes were sampled from September 2020 to Feburary 2021 using Centers for Disease Control and Prevention (CDC) light trap and Pyrethrum Spray Catch (PSC). Moreover, mosquito larvae were also collected from different breeding sites and reared to adults to assess susceptibility status of populations of An. gambiae s.l. in the study area. Susceptibility tests were conducted on two to three days old non blood fed female An. gambiae s.l using insecticide impregnated papers with deltamethrin (0.05%), alpha-cypermethrin (0.05%), propoxur (0.1%), pirimiphos-methyl (0.25%) and bendiocarb (0.1%) following World Health Organization (WHO) standard susceptibility test procedure. Molecular diagnostics were done for the identification of member species of An. gambiae s.l and detection of knockdown resistance (kdr) allele using species specific polymerase chain reaction (PCR) and allele specific PCR. Results: In total, 468 adult mosquitoes were collected from different houses. Culex mosquitoes were the most dominant (80.4%) followed by Anopheles mosquitoes. Three species of Anopheles mosquitoes (An. coustani, An. pharoensis, and An. gambiae (s.l.)) were identified, of which An. coustani was the dominant (8.1%) species. WHO bioassay tests revealed that the populations of An. gambiae s.l in the study area are resistant against alpha-cypermethrin and deltamethrin whereas, susceptible to bendiocarb, pirimiphos-methyl and propoxur. Out of the total 86 An. gambiae s.l specimens assayed, 79 (92%) successfully amplified and identified as An. arabiensis. West African Kdr (L1014F) mutation was detected with high Kdr allele frequency ranging from 67-88%.Conclusion: The detection of target site mutation, kdr L1014F allele, coupled with the phenotypic resistance against alpha-cypermethrin and deltamethrin call for continuous resistance monitoring.
First Detection of Rotavirus C in Asymptomatic Pigs from India with Unexpected Dominance and Characterization of the Structural and Nonstructural Genes
Authors: Joshi Madhuri S , Arya Shalu A. , Shinde Manohar S. , Gopalkrishna Varanasi 
Published: December 2021

Abstract
Epidemiological and molecular investigation was conducted on Rotavirus C (RVC), the viral agent documented with rising prevalence rate, disease severity and cross species transmission and large outbreak potential. Fecal specimens of pigs collected from two cities of Maharashtra state, India tested for RVC showed 20.1% detection rate with majority below 3 months of age. No significant difference in detection rates was observed in the specimens collected in 2009 from Northern and in 2013 from Western parts of Maharashtra. The phylogenetic analyses showed presence of the I7 and I10 genotypes of the VP6 gene and representative strains with G1 and E5 genotypes of the VP7 and NSP4 gene respectively. Full genome characterization of a single strain showed presence of G1, P1, I7, R1, C1, M3, A1, N5, T5, E5, H1 genotypes of the VP7, VP4, VP6, VP1, VP2, VP3, NSP1, NSP2, NSP3, NSP4 and NSP5 genes respectively. This is the first evidence of detection of porcine RVC in asymptomatic pigs in India as well as with highest detection rate reported in asymptomatic pigs till date globally. Identification of porcine RVC at two time intervals and two different parts of Maharashtra state indicates the possibility of continuous circulation of RVC in pig population through asymptomatic infections.
A Tumor-Infiltrating Lymphocyte-Based Risk Model Predicts Prognosis of Colon Cancer
Authors: Sui Ziqi , Zhu Yanli , Wu Kejia , Wang Shuxiang , Yuan Xixian , Wang Shuqiu 
Published: December 2021

Abstract
Tumor-infiltrating lymphocytes are relevant to the tumor prognosis and response to immunotherapy in colon cancer. The gene expression data of colon cancer was obtained from the cancer genome atlas (TCGA) database and the components of immune cell types were analyzed by CIBERSORT. Selection operator (LASSO) and multivariate Cox regression filtered immune cells and selected the most significant cell types to construct an immune risk model including memory B cells, plasma cells, T follicular helper (Tfh) cells, M0 macrophages and resting dendritic cells. Receiver operating characteristic (ROC) curves were used to verify the sensitivity and specificity of the model, which was validated in Gene Expression Omnibus (GEO) dataset. Combined with the clinical traits, a nomogram was established to predict the prognosis of colon cancer. According to function analyses through weighted correlation network analysis (WGCNA) and gene set enrichment analysis (GSEA), tumor-infiltrating immune cells showed significant importance in tumor immune-associated regulation, especially the adhesion, migration and invasion in colon cancer.
Expression of Thrombin-Cleaved Osteopontin and Integrin α9 and β1 Signaling Pathway Molecules in Chronic Subdural Hematomas
Authors: Osuka Koji , Ohmichi Yusuke , Ohmichi Mika , Suzuki Chiharu , Aoyama Masahiro , Iwami Kenichiro , Watanabe Yasuo , Miyachi Shigeru 
Published: December 2021

Abstract
Chronic subdural hematoma (CSDH) is considered to be an inflammatory and angiogenic disease. Osteopontin is an extracellular matrix protein. Osteopontin is cleaved by thrombin, resulting in N-half osteopontin, which is more prominent in integrin signal transduction. We examined the expression of N-half osteopontin in the CSDH fluid and the expression of integrin α9 and β1 and the downstream components of the angiogenic signaling pathways in the outer membrane of CSDHs. Twenty samples of CSDH fluid and 8 samples of CSDH outer membrane were included. The concentrations of N-half osteopontin in the CSDH fluid were measured using ELISA kits. The expression of integrin α9 and β1, vinculin, talin-1, focal adhesion kinase (FAK), paxillin, α-actin, Src and β-actin was examined by western blot analysis. The expression of integrin α9 and β1, FAK and paxillin was also examined by immunohistochemistry. We investigated whether CSDH fluid could activate FAK in cultured endothelial cells in vitro. The concentration of N-half osteopontin in CSDH fluid was significantly higher than that in the serum. Western blot analysis revealed above-mentioned molecules. In addition, integrin α9 and β1, FAK and paxillin were localized in the endothelial cells of vessels within the CSDH outer membrane. FAK was significantly phosphorylated immediately after treatment with CSDH fluid. Our data suggest that N-half osteopontin in CSDH fluid promotes neovascularization in endothelial cells through integrins α9 and β1. The N-half osteopontin and integrin signaling pathway might be a useful therapeutic target for treating the growth of refractory CSDH.
Nonequilibrium Band Occupation and Optical Response of Gold After Ultrafast XUV Excitation
Authors: Ndione Pascal D. , Weber Sebastian T. , Gericke Dirk O. , Rethfeld Baerbel 
Published: December 2021

Abstract
Free electron lasers offer unique properties to study matter in states far from equilibrium as they combine short pulses with a large range of photon energies. In particular, the possibility to excite core states drives new relaxation pathways that, in turn, also change the properties of the optically and chemically active electrons. Here, we present a theoretical model for the dynamics of the nonequilibrium occupation of the different energy bands in solid gold driven by exciting deep core states. The resulting optical response is in excellent agreement with recent measurements and, combined with our model, provides a quantitative benchmark for the description of electron-phonon coupling in strongly driven gold. Focusing on sub-picosecond time scales, we find essential differences between the dynamics induced by XUV and visible light.
A Novel NIR-II Theranostic Agent: A Win–win Strategy of Tracing and Blocking Tumor-associated Vessels for Oral Squamous Cell Carcinoma
Authors: Wei Zheng , Zhang Hongbo , Zou Huihui , Song Chuanhui , Zhao Sufeng , Zhao Zichen , Zhang Xinyu , Zhang Guorong , Cai Yu , Han Wei 
Published: December 2021

Abstract
Tumor vessel co-option is a crucial predictor for tumor invasiveness but is easy to ignore in tumor vascular targeting therapy. A high density of tumor vessel co-option located alongside the tumor frontier predicted a high tendency of tumor invasion and regional metastasis. Herein, a novel NIR-II nanoagent (CS NPs) was constructed with an organic COi8DFIC dye and sorafenib, which demonstrated high tissue penetration and low tissue autofluorescence, enabling imaging of vessel co-option and tumor micrometastasis. This nanotherapeutic agent displayed considerably improved quantum yield of fluorescence (0.89%), high ROS generation and fairly good biosafety in vivo. Compared with indocyanine green (ICG), CS NPs exhibited better photostability and photothermal conversion efficiency. By tracking tumor-associated vessels and real-time tumor imaging, CS NPs could open/stop vascular targeting therapy by laser on/off. The combination of vessel disruption and imaging-guided photothermal therapy/photodynamic therapy provided a win–win strategy for oral squamous cell carcinoma (OSCC).
Viral Haemorrhagic Fevers and Malaria Co-Infections Among Febrile Patients Seeking Health Care in Tanzania
Authors: Rugarabamu Sima , Rumisha Susan F. , Mwanyika Gaspary O. , Sindato Calvin , Lim Hee-Young , Misinzo Gerald , Mboera Leonard E.G. 
Published: December 2021

Abstract
Background: In recent years there have been reports of viral haemorrhagic fever (VHF) epidemics in Sub-Saharan Africa where malaria is endemic. VHF and malaria have overlapping clinical presentations making differential diagnosis a challenge. The objective of this study was to determine the prevalence of selected zoonotic VHFs and malaria co-infections among febrile patients seeking health care in Tanzania. Methods: This facility-based cross-section study was carried out in Buhigwe, Kalambo, Kyela, Kilindi, Kinondoni, Kondoa, Mvomero, and Ukerewe districts in Tanzania. The study involved febrile patients seeking health care from primary healthcare facilities. Blood samples were collected and tested for infections due to malaria, Crimean-Congo haemorrhagic fever (CCHF), Ebola virus disease (EVD), Marburg virus disease (MVD), Rift Valley fever (RVF) and yellow fever (YF). Malaria infections were tested using rapid diagnostics tests while exposure to VHFs was determined by screening for immunoglobulin M antibodies using commercial enzyme-linked immunosorbent assays. Results: A total of 308 participants (mean age=35±18.9 years) were involved in the study. Of these, 54 (17.5%) had malaria infection and 15 (4.8%) were positive for IgM antibodies against VHFs (RVF=8; CCHF=2; EBV=3; MBV=1; YF=1). Six (1.9%) individuals had both VHF (RVF=2; CCHF=1; EVD=2; MVD=1) and malaria infections. The highest co-infection prevalence (0.6%), was observed among individuals aged 46-60 years (p<0.05). District was significantly associated with co-infection (p<0.05) with the highest prevalence recorded in Buhigwe (1.2%) followed by Kinondoni (0.9%) districts. Headache (100%) and muscle, bone, back and joint pains (83.3%) were the most significant complaints among those infected with both VHFs and malaria (p=0.001). Conclusions: Co-infections of VHF and malaria are prevalent in Tanzania and affect more the older than the younger population. Since the overlapping symptoms in co-infected individuals may challenge accurate diagnosis, adequate laboratory diagnosis should be emphasized in the management of febrile illnesses.
Prognostic Impact of lncRNA MCM3AP-AS1 Expression in Malignant Solid Tumors: A Systematic Review and Meta-analysis
Authors: le qi , chen wei , Jiang Lei , Hu Wenwen , Yao Nan 
Published: December 2021

Abstract
Objectives: MCM3AP-AS1 is a newly discovered long non-coding RNA that functions as a biomarker in many different cancer types. However, the pooled role of lncRNA MCM3AP-AS1 in the prognosis of human cancers remains unclear. We performed a systematic review and meta-analysis to explore its potential prognosis for malignant tumors. Materials and methods: A literature survey was conducted by searching in the PubMed, Embase, Web of Science, China National Knowledge Infrastructure and Wanfang databases for studies published as of September 1, 2021. The pooled hazard ratio (HR) and 95% confidence interval (95% CI) were calculated to evaluate the relationship between MCM3AP-AS1 expression and overall survival (OS). The endpoints also included various clinical parameters. Results: A total of 14 studies containing 921 cancer patients were finally included into this meta-analysis. The results comprehensively showed that increased MCM3AP-AS1 expression was significantly correlated with poor overall survival (HR = 1.83, 95% CI: 1.56-2.14, P<0.00001). A subgroup meta-analysis for overall survival was conducted. Additionally, high level of lncRNA MCM3AP-AS1 was significantly associated with worse differentiation (OR = 1.76, 95 % CI: 1.12–2.75, P = 0.01), larger tumor size (OR = 2.70, 95 % CI: 1.13–6.46, P = 0.03), advanced clinical stage (OR = 2.52, 95 % CI: 1.32–4.81, P = 0.005) and lymph node metastasis (OR = 2.85, 95 % CI: 1.16–7.00, P = 0.02), respectively. Conclusions: LncRNA MCM3AP-AS1 might be a potential and unfavorable prognostic biomarker of cancer.
Explaining the Challenges of Coping with Coronavirus Crisis in the Workplaces: A Qualitative Study
Authors: Tajvar Abdolhamid , Hosseini Zahra , Farahbakhsh Mohammadreza , Fakherpour Anahita , homayuni atefeh 
Published: December 2021

Abstract
Background: Workplaces play a highly important role in controlling or spreading the prevalence of COVID-19. Therefore, the lack of proper implementation of hygienic protocols in theses environments might increase the risk of getting infected with the COVID-19 among the employees, following the increase of the COVID-19 in the family and community levels. This qualitative study was aimed to explain the challenges of coping with coronavirus crisis in the workplaces.Methods: The present qualitative study was conducted with a conventional content analysis approach. We used the purposeful sampling with maximum diversity in terms of working processes. Data were collected through semi-structured interviews. Interviews were performed to the point of information saturation. MAXQDA software version 10 was used for data management.Results: Through the content analysis of the interviews with the participants, we identified 2 main classes and 9 sub-classes. The main classes included problems and limitations related to the intra-organizational factors (job nature, budget allocation problems, individual factors, disorganization, manpower and equipment, and Lack of commitment and insufficient support of managers) and extra-organizational factors (lack of accessibility to valid information, black market, and inter-sectorial coordination problems). Conclusion: The study findings indicated that organizations and industries face numerous internal and external challenges in coping with the COVID-19 pandemic. Paying attention to the mentioned limitations and attempting to eliminate them; especially, by the governmental organizations, employers, and managers could help in effective confronting the COVID-19 pandemic.
Atmospheric Modes Excited by the 2021 August Eruption of the Fukutoku-okanoba Volcano, Izu-bonin Arc, Observed as Harmonic Oscillations of QZSS Total Electron Conte
Authors: Heki Kosuke , Fujimoto Tatsuya 
Published: December 2021

Abstract
Continuous Plinian eruptions of volcanoes often excite atmospheric resonant oscillations with several distinct periods of a few minutes. We detected such harmonic oscillations excited by the 2021 August eruption of the Fukutoku-Okanoba volcano, a submarine volcano in the Izu-Bonin arc, in ionospheric total electron content (TEC) observed from global navigation satellite system (GNSS) stations deployed on three nearby islands, Chichijima, Hahajima, and Iwojima. Continuous records with the geostationary satellite of Quasi-Zenith Satellite System (QZSS) presented four frequency peaks of such atmospheric modes. The harmonic TEC oscillations, started at ~5:16 UT, exhibited an unprecedented large amplitude but decayed in a few hours.
Increased Expression of Integrin Subunit α3 is Correlated with Worse Recurrence-Free Survival in Thyroid Cancer
Authors: Ma Jun-jie , Xiang Cheng , Zhu Heng-qing , Bai Bing-long , Wang Ping 
Published: December 2021

Abstract
Objective: To investigate the potential effect of integrin subunit α3 (ITGA3) on thyroid cancer (THCA). Materials and Methods: Based on bioinformatics databases, the expression levels of ITGA3 were firstly analyzed, followed by evaluating the prognostic significance of ITGA3 in THCA patients. Cox regression analysis predicted the independent prognostic factors for THCA. Then, cBioportal and GSCA databases were applied to evaluate genetic alterations of ITGA3. Functional enrichment analysis was conducted using the R package. Finally, the upstream microRNAs of ITGA3 were determined. Results: ITGA3 gene and protein levels were higher in the THCA group than normal group (all P <0.05). And ITGA3 mRNA expression was significantly related to cancer stage and histological subtype (all P <0.01). Moreover, high expression of ITGA3 was associated with poor recurrence-free survival (RFS) of THCA patients in all subgroups (all P <0.01). Cox regression analysis presented that ITGA3 overexpression was an independent prognostic factor for worse RFS in THCA patients. Besides, significant relations were observed between ITGA3 and genetic alterations (FDR <0.01). Functional enrichment analysis indicated ECM-receptor interaction, and cell adhesion molecules were the shared regulatory pathways. We also found that ITGA3 might be the target gene of hsa-miR-3129, hsa-miR-181d, hsa-miR-181b, hsa-miR-199a, and hsa-miR-199b, which were all correlated with THCA patient prognosis. Conclusions: ITGA3 could be a reliable biomarker and promising therapeutic target for improving the diagnosis and clinical outcomes of THCA patients.