bluuebunny/crossref_metadata_2025_split huggingface dataset:
Antioxidant activity of casein yogurt against dioxin toxicity in rats liver
Authors: Setianingrum A , Haskito A E P 
Published: May 2021

Abstract
Prolonged exposure to dioxin can lead to negative effects. Oxidative stress is caused by dioxin toxicity due to interference of the metabolism process including lipid metabolism. This current study was to address the role of casein yogurt in the prevention of oxidative stress caused by dioxin intoxication. Substances used were 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) diluted in corn oil and casein from goat milk yogurt. A total of 24 Wistar rats divided into 6 groups: control, placebo with 600 mg/kg BW of casein, TCDD group with 100 ng/kg BW of TCDD, T1 to T3 groups were given with TCDD and casein in different dose: 300, 600, and 900 mg/kg BW. The respective dose was given orally daily for 21 days. Blood serum was collected for cholesterol and triglyceride analysis. Liver samples were collected for superoxide dismutase (SOD) activity assay. Statistic analysis using one-way ANOVA and post hoc Tukey test. Results showed that casein yogurt increased SOD activity in TCDD intoxicated rats. A significant increase (P<0.05) was shown by the group with 600 and 900 mg/kg BW of casein. Serum lipid profile exhibited no significant change in serum cholesterol and triglyceride level. Casein yogurt is potential to prevent cellular damage caused by dioxin toxicity.
Evaluation of the growth and yield of true shallot seed on the application of two technology packages during two planting seasons in Central Sulawesi
Authors: Saidah  , Muchtar  , Wahyuni A N , Padang I S , Syafruddin  , Irmadamayanti A 
Published: May 2021

Abstract
True shallot seed (TSS) considered to be used due to their technical and economis feasibility. The aim of the study was to evaluate the growth and yield productivity of TSS and shallot bulbs generated from TSS. The study was conducted in Kotarindau Village, Dolo District, Sigi Regency, Central Sulawesi. The seeds was planted on dry lowland area. The experiment was arranged using a Randomized Block Design with two treatments, namely the Introduction Technology Package and Farmer Technology Package during two planting seasons. Each treatment was repeated 13 times. Parameter observed were the growth and yield of shallot. The data obtained were analyzed using the T test. The results showed that the Introduction Technology Package had a significant effect on several components of shallot growth and yield compare with the Farmer’s package in the first (using seeds) and the second season (using bulbs). In the second planting season, the number of bulbs was 7-9 times higher than in the first planting season, and the productivity was 2-3 times higher. We can conclude that the shallot bulbs harvested from the seeds have the opportunity to be used as a source of seeds for the next planting season.
Construction of a full length α-factor secretory signal sequence for human insulin precursor expression in Pichia pastoris
Authors: Utami N , Nurdiani D , Hariyatun  , Putro E W , Kusharyoto W 
Published: May 2021

AbstractSaccharomyces cerevisiaeandPichia pastorisare yeast known as a potential expression system to produce recombinant protein. The full-length α-factor (α-mating factor) secretory signal ofS. cerevisiaeplays an essential role in the secretion and processing of the mature protein of interest. Here, we attempted to construct the full-length α-factor signal sequence ofS. cerevisiaein the pD902-IP (Insulin Precursor) expression vector for secreted expression of human insulin precursor inP. pastoris. We have isolated the full-length α-factor secretory signal sequence in a pTA2 cloning vector. The full-length α-factor was then inserted into the IP-cassette of pD902-IP and transformed intoE. coliTOP10. TheE. colitransformants, which were able to grow on the Zeocin selection medium, harbored the full-length α-factor for the IP expression in the pD902 vector validated by PCR and sequencing. Furthermore, the construct electroporation intoP. pastorisX-33 was done and followed by IP protein expression confirmation visualized with SDS-PAGE.
Resistant performance of T10 Rojolele transgenic rice events harboring cry1B::cry1Aa fusion genes against the rice yellow stem borer Scirpophaga incertulas Wlk
Authors: Nugroho S , Sari D I , Zahra F , Rachmawati S , Maulana B S , Estiati A 
Published: May 2021

Abstract
Rice yellow stem borer (Scirpophaga incertulas Wlk) (rice YSB) is one of the most important insect pests in rice (Oryza sativa L.). Plant damage caused by the pest attack in all growth stages could significantly reduce yield. Cry toxins from Bacillus thuringiensis are known to be effective against rice YSB, therefore transgenic rice events cv Rojolele harbouring the cry1B::cry1Aa fusion genes driven by the maize ubiquitin promoter have been developed to improve rice resistance. To determine that the resistant traits has been stably inherited and expressed, in-planta and in-vitro bioassays of the T10 generation were performed on 6 independent transgenic rice events using the 1st instar larvae of rice YSB. In-planta bioassay was performed on 5-week-old rice plants grown in pots in a transgenic glasshouse, in 7 replicates. Each plant was invested with 20 1st instar larvae of rice YSB. The in-vitro bioassay was performed using the stems of the transgenic rice events against 10 1st instar larvae of rice YSB, in aerated Petri dishes in a culture room under room temperature, in three replicates. Results showed that the T10 generation of the transgenic rice events stably maintained the transgene integration and their resistance against the rice YSB.
Construction of a recombinant vector containing SAI gene using Gateway-based RNAi system: a preliminary study in the development of high sucrose sugarcane
Authors: Hadiarto T , Riyanti E I , Listanto E , Polosoro A 
Published: May 2021

Abstract
Recombinant plasmid construction that produces hairpin molecules for gene knockdown is not an easy method. Gateway cloning system is a technology to transfer DNA fragments utilizing clonase and specific sites. This study aimed to construct RNAi expression vector containing soluble acid invertase (SAI) gene using Gateway system to produce high-sugar content sugarcane. PCR was conducted to generate specific SAI fragment using cDNA template from two sugarcane varieties: Bululawang and PSJK922. Of the two specific primer pairs designed, only one pair amplified the SAI 161 bp gene fragment. Amplification occurred in both cDNA samples. TA (Thymine-Adenine pair) cloning was performed with pCR8/GW/TOPO plasmid and a clonase reaction was carried out to insert the specific fragments into the pHELLSGATE 8 hairpin plasmid. The results showed that two of the four colonies analyzed by associated restriction enzymes had plasmid pHELLSGATE8::hpSAI that contained a hairpin component of the SAI fragment. One attempt to construct the hairpin RNA using Gateway system in this study indicates the easiness of the system when compared to traditional cloning. The DNA sequence in the hairpin portion of this plasmid will be analysed to confirm SAI gene fragment, and it will be followed by transformation of sugarcane and SAI characterization.
In vitro growth response of Patchouli (Pogostemon cablin) cultured in medium containing Methylobacterium spp. filtrate
Authors: Kosmiatin M , Husni Ali , Salma Selly 
Published: May 2021

Abstract
Mass seeds production through micropropagation can support modern agriculture, but this technique is expensive and largely determined by a medium formulation. The addition of synthetic Plant Growth Regulator (PGR) has a residual effect, meanwhile natural PGRs is more secure but expensive. In vitro testing of PGR activity obtained from bacteria was the first step to substituted synthetic PGRs to become nature PGR. Research to observe PGR activity obtained from four strains of Methylobacterium spp that had been analyzed of the capability of PGRs synthesis was done on in vitro patchouli cultured. There are three activities: 1) The sterilized technique of filtrate using filtration techniques, humid heat, and a combination of both; 2) Testing of Methylobacterium spp. from that production of cytokinins and compared its activity with commercial PGRs (BA and zeatin); and 3) auxin (NAA and IAA). The research showed that strain TD-J2 filtrate added to medium was similar to BA activities, and TD-J7 was similar to zeatin. Auxin activity derived from strain TD-J10 showed the capacity to induces root formation. The research result indicates that synthesis PGR derived from Methylobacterium spp. has similar activity with commercial PGRs.
Influence of dyeing condition on cloth color by yellow dyes from mixed fungi grown in different culturing media
Authors: Suciatmih  
Published: May 2021

Abstract
In the present study, cloth was dyed with yellow dyes obtained from a mixture of Aspergillus and Paecilomyces fungi grown in three different media, three mordant techniques, six mordant types and four dyeing repetitions. By matching with the Royal Horticultural Society (RHS) color chart, the range of colors developed on the dyed cloth was measured. The results showed that medium-1 and medium-3 produced stronger dyed cloth colors than medium-2; and different mordant types and techniques each also produced more varied cloth colors from both media than did medium-2. Meta- and pre-mordant using different mordant types applied to the dyes from all of the media each produced more varied cloth colors than did post-mordant; while mordant technique using Cu+2 and Fe+2 in the same media each produced more varied cloth colors than did other mordant types. The optimum condition for the cloth dyeing by the dyes in medium-1 and medium-3 added with pre-mordant Cu+2 was 3 times, while with meta-mordant Cu+2 was 3 and 5 times respectively. The optimum condition for the cloth dyeing by the dyes in medium-1 and medium-3 added with pre-mordant Fe+2 each was 1 time, while with meta-mordant Fe+2 was 1 and 5 times, respectively.
Effect of Feammox on Landfill Leachate Treatment and Its Influence on pH
Authors: Wang Bing , Sun Bing , Liu Yunlong , Yang Lin 
Published: May 2021

Abstract
Feammox is a new biological nitrogen removal process which can synchronously achieve NH4
+-N oxidation and Fe3+ reduction under the action of microorganisms. In order to investigate the starting of Feammox and the influence of different pH on the denitrification effect of simulated landfill leachate wastewater, the Feammox reaction was carried out by inoculating Anammox sludge under anaerobic conditions, and the N/Fe conversion pathway in the reaction was observed. On the 43rd day, the influent Fe3+ concentrations in the reactor was increased, and it was found that it promoted Feammox. Taking Feammox sludge in good operation as inoculation sludge, pH was set at 3.5-7.5, respectively. The results showed that the landfill leachate had the best denitrification effect when pH =4.5, and the Feammox reaction almost did not exist in the reactor when pH =3.5
Research method for hydrodynamic characteristics of tidal current turbine
Authors: Xu Junhui 
Published: May 2021

Abstract
Tidal energy is a very rich Marine resource, which has high energy concentration, small geometric scale and strong predictability. The research status, development and utilization of tidal current turbine at home and abroad are expatiated, and two research methods for hydrodynamic characteristics of tidal current turbine, including numerical calculation and simulation experiment, are introduced, which are of certain significance to the performance optimization of tidal current turbine.
Variation regular and influencing factors of pore and permeability parameters of each oil layer of block A
Authors: Yanfeng Bao 
Published: May 2021

Abstract
The porosity and effective permeability values of an oil layer in different periods of the same block are quite different, It is proposed to take the actual production target interval and small block in block A as the unit, by analyzing the results of laboratory water flooding experiments and using the data of coring wells and exploration wells in the study area, as well as the data of static database, the variation rules of porosity and effective permeability in different development stages are summarized. After comprehensive study, it is found that the change of parameter interpretation model and water injection development are the main factors for the change of hole and permeability parameters of well completion logging interpretation in different periods in block A.
Study on percolation mechanism of low permeability reservoir
Authors: Yuan Wang Wen 
Published: May 2021

Abstract
The development practice of low permeability reservoir shows that there is start-up pressure gradient in low permeability reservoir, and the lower the permeability is, the higher the start-up pressure gradient is. Therefore, there are different types of sandstones in low-permeability sandstone reservoirs, with different permeability and different starting pressure gradient, resulting in different seepage space. This paper attempts to establish mathematical model and numerical simulation model to simulate the movement law of oil and water in low permeability reservoir by considering different seepage space and its starting pressure, so as to study the distribution and variation law of invalid water injection in space, and provide basis for formulating reasonable injection production ratio and other injection production policies.
Exploration and Enhancement of Tujia Nationality’s Original Ecological Packaging Sustainable Design in Western Hube
Authors: Liu Lin , Fang Fengming 
Published: May 2021

Abstract
Original packaging design of Western Hubei Tujia is a handmade household item of Tujia people, and it is also a packaging container used to store, protect, and transfer living materials. The original ecological packaging origin, modelling materials, patterns, and colours of the Tujia nationality in western Hubei were reconstructed and sorted out under the guidance of sustainable design principles, from the perspective of sustainable design exploration and sustainable design continuation. These local materials were combined, in order to examine and interpret the spiritual significance and material function of the handmade packaging container. The prospects of development brought by the integration of the original ecological packaging of the Tujia family and modern design are explored, so that it further injects new life and energy into sustainable designs. While reflecting the advantages of original ecological packaging shape, material and rich ethnic elements, it focuses on the continuation and innovation of sustainable design of the Tujia native food packaging, promotes the ecological concept, develops sustainable packaging, and provides a creative space for Tujia family packaging design ideas.
Optimization of pressure drop model for shale gas wells in area X based on correlation coefficient method
Authors: Wang Haitao , Xia Lian 
Published: May 2021

Abstract
The bottom hole pressure is an important parameter in the production process of a gas well, and it is of great significance to many aspects such as gas well productivity evaluation, dynamic analysis and production allocation. A large number of shale gas wells in area X were lack of the bottom hole pressure data. In order to solve this problem, based on extensive research in many articles, this paper selected eight kinds of calculation models as candidate objects, such as Hagadorn-Brown and Beggs-Brill. Different models were used to calculate the bottom hole pressure of only two shale gas wells with measured pressure data in this area, and the calculated results were compared with the measured data to select the best model for calculating the bottom hole pressure of shale gas wells in area X. In the optimization analysis, it was found that the conventional average relative error comparison method or the graph comparison method could not reflect the application situation of the calculation models well in this area. Therefore, on the basis of the two comparison methods, the correlation coefficient method was introduced to quantify the overall difference between the calculated pressure and the measured pressure. The results showed that the calculated result of Orkiszewski model fitted the measured pressure best.
New research progress of large capacity new synchronous condenser
Authors: He Shan , Cheng Jing , Yuan Zhi , Dong Xinsheng , Zhang Chuanmin , Wang Weiqing 
Published: May 2021

Abstract
With the increase of HVDC(high voltage direct current) transmission system, the new large-capacity synchronous condenser is the most suitable device to meet the dynamic reactive power demand of the power grid system. Therefore, it has gradually become one of the research hot topics. Based on this, the paper systematically summarizes the research status of the new synchronous condenser. Firstly, the operation principle of the synchronous condenser is introduced from four aspects: its reactive power characteristics, operation state and the typical type and control strategy. Secondly, by comparing it with power electronic reactive power compensation equipment, the superior dynamic reactive power output characteristics of the new synchronous condenser are expounded, and the influence factors are analysed, the example that stable grid voltage is given. Finally, the latest development of the state monitoring technology of the synchronous condenser is summarized from both the existing fault diagnosis technology and parameter identification technology. In this paper, the basic theory, control strategy and operation state monitoring of the new synchronous condenser are summarized, which could provide reference for the further research.
Research on the Application of Green Ecological Concept in the Architectural Design of Kindergartens
Authors: Fan Bei , Li Runkun 
Published: May 2021

Abstract
Integrating green ecological concepts and design into the kindergarten architectures is a significant subject and full of challenges. Only by leading children to be close to nature and learning and living in a green and ecological kindergarten environment can we provide better education for children as much as possible. This article discusses the existing problems in the architectural design of kindergartens in our country, and studies the application principles of the green ecological concept in the architectural design of kindergartens at first. Then it takes the China’s Haichao Kindergarten as an example to analyze how the green ecological concept can be better used in the architectural design of kindergartens, and explores the design ideas and methods of Haichao Kindergarten. Finally, it summarizes the importance of green ecological design concepts in today’s kindergarten architectures.
Influence of Energy Saving and Emission Reduction on Innovation of Science, Technology, Industry and Enterprises in China
Authors: Gao Huan , Sun Bo 
Published: May 2021

Abstract
Energy saving and emission reduction policy is a long-term strategic choice in China, which will have a far-reaching impact on science and technology, economy and enterprise innovation. By controlling the growth rate of industries with high energy consumption and high pollution, the policy of energy conservation and emission reduction promotes the transformation of China’s economic growth mode to resource conservation and clean environment. Service industry has the characteristics of low energy consumption and light pollution, which will be paid special attention to. The policy of energy conservation and emission reduction with promising medium and long-term prospects is a long-term strategic choice for China, which will have a far-reaching impact on the economy. During the 30 years of reform and opening up, traditional industries with high energy consumption, such as electric power, metallurgy, cement and chemical industry, have made great contributions to China’s economic development by ensuring supply. Today, China has entered a new historical development stage, and the extensive growth mode with high energy consumption and high pollution is increasingly facing challenges. The intensive growth mode with the core of improving the utilization efficiency of production factors such as energy and the low-carbon economy with low energy consumption, low pollution and low emission have become the future economic development direction of China.
Comparison of sample temperature effect on femtosecond and nanosecond laser-induced breakdown spectroscopy
Authors: LIU Miao , CHEN Anmin , CHEN Yutong , ZENG Xiangyu , WANG Qiuyun , ZHANG Dan , YANG Dapeng , JIN Mingxing 
Published: May 2021

AbstractIn this paper, we investigated the emission spectra of plasmas produced from femtosecond and nanosecond laser ablations at different target temperatures in air. A brass was selected as ablated target of the experiment. The results indicated that spectral emission intensity and plasma temperature showed similar trend for femtosecond and nanosecond lasers, and the two parameters were improved by increasing the sample temperature in both cases. Moreover, the temperature of nanosecond laser-excited plasma was higher compared with that of femtosecond laser-excited plasma, and the increase of the plasma temperature in the case of nanosecond laser was more evident. In addition, there was a significant difference in electron density between femtosecond and nanosecond laser-induced plasmas. The electron density for femtosecond laser decreased with increasing the target temperature, while for nanosecond laser, the electron density was almost unchanged at different sample temperatures.
Measuring the impact of a Chronic Obstructive Pulmonary Disease Community Respiratory Programme on emergency admissions to hospital: a controlled interrupted time series analysis
Authors: Levin Kate A , Milligan Marianne , Bayes Hannah K , Crighton Emilia , Anderson David 
Published: May 2021

Abstract

Background
A community respiratory service was implemented in the North West of Glasgow (NW) in January 2013, as part of the Reshaping Care for Older People programme (RCOP). This study aimed to measure the impact of the service on older people’s emergency admissions (EAs) to hospital.

Methods
EAs to hospital with a primary diagnosis of COPD (COPD EAs) per 1,000 population aged 65 years+ in NW were compared before and after onset of the service with a 6-month phase-in period, using segmented linear regression. South and North East Glasgow (S + NE) was the control—an area with no such service in place. The model adjusted for the rate of all-cause EAs to control for the impact of other localised RCOPP initiatives. Autoregressive terms and a Fourier term to adjust for seasonality were included in the model.

Results
Prior to implementation of the respiratory service, increases in COPD EAs over time were evident in NW. Adjusting for changes in COPD EAs in NE + S, an additional reduction of −0.04 (−0.03, −0.05) per 1,000 population per month was observed in NW following the phase-in, so that by March 2015, the predicted reduction due to the respiratory service was −0.85 COPD EAs per 1,000 population, a relative reduction of 34.3%. No significant changes in admissions with COPD as a secondary diagnosis (COPD5 EAs) were observed, suggesting that the intervention had no impact on these.

Conclusions
The community respiratory service was associated with a significant reduction in the rate of COPD EAs among older people and no change in COPD5 EAs.