bluuebunny/crossref_metadata_2025_split huggingface dataset:
Trace Elements In The Detoxifying And Accumulating Body Parts of Mytilus Galloprovincialis Lamark, 1819 (Crimea, Black Sea): Human Health Risks And Effect of The Sampling Site Location
Authors: Chelyadina Natalya S. , Kapranov Sergey V. , Popov Mark A. , Smirnova Lyudmila L. , Bobko Nikolay I. 
Published: December 2021

Abstract
The mussel M. galloprovincialis is one of the most popular species in world’s mariculture and environment pollution bioindicators. Although this mollusk was in a focus of numerous publications, the detoxifying and accumulating role of some of its body parts was insufficiently studied. In the present work, using inductively coupled plasma mass spectrometry, the distribution of Cr, Co, Ni, Cu, Zn, As, Cd, Hg, Pb in soft tissues, byssus, and shell liquor of this bivalve was studied for the first time as a function of sampling location in the Black Sea off southwestern Crimea. Multivariate analysis showed significant differences in the overall distribution of the elements among the body parts and sampling sites under consideration. Human health risks from the consumption of this mollusk were shown to be unlikely. Byssus of M. galloprovincialis was characterized as an ideal indicator of marine environment pollution with Ni, Cu, Pb, Сo, and Cr as it contained the highest contents of these elements among the body parts under study (Ni up to 91%, Cu and Pb up to 79%). For the first time, the concentrations of trace elements were determined in the shell liquor and its role as the trace element excretion system was demonstrated. In the shell liquor, the largest share among the body parts was that of Hg (up to 71%). In soft tissues, Zn, Cd and As were in largest proportions among the body parts (63, 62, and 54%, respectively). The trace element contents in soft tissues of M. galloprovincialis decreased in the following order: Zn > Cu > As > Ni > Pb > Cd > Cr > Co > Hg.
Metabolite Patterns of Patients With Peripheral Arterial Disease In Response To Exercise
Authors: Bellomo Tiffany R. , Tsao Noah L. , Johnston-Cox Hillary , Borkowski Kamil , Shakt Gabrielle , Judy Renae , Ractcliffe Sarah J. , Fiehn Oliver , Floyd Thomas F. , Wehrli Felix W. , Newman John W. , Damrauer Scott M. 
Published: December 2021

Abstract
Background: The progression of peripheral arterial disease (PAD) and the metabolome’s response to supervised exercise therapy (SET) has not yet been investigated. Results: When analyzing individual time points during exercise, we found high levels of anandamide (AEA) before any exercise interventions were associated with a shorter, or worse, walking time. We also found increased arachidonic acid (AA) and decreased levels of AA precursors dihomo-γ-linolenic acid and diacylglycerol before any exercise was associated with shorter walking times. Participants who were able to tolerate large increases in AA during acute exercise had longer, or better, walking times both before and after SET. Significance: We identified two pathways of relevance to individual response to SET: AEA synthesis may increase the activity at endocannabinoid receptors, resulting in worse treadmill test performance. SET may help train patients withstand higher levels of AA and inflammatory signaling, resulting in longer walking times.
Electrical Control of Quantum Emitters in a Van der Waals Heterostructure
Authors: White Simon , Yang Tieshan , Dontschuk Nikolai , Li Chi , Xu Zaiquan , Kianinia Mehran , Stacey Alastair , Toth Milos , Aharonovich Igor 
Published: December 2021

Abstract
Controlling and manipulating individual quantum systems in solids underpins the growing interest in development of scalable quantum technologies1, 2. Recently, hexagonal boron nitride (hBN) has garnered significant attention in quantum photonic applications due to its ability to host optically stable quantum emitters3-7. However, the large band gap of hBN and the lack of efficient doping inhibits electrical triggering and limits opportunities to study electrical control of emitters. Here, we show an approach to electrically modulate quantum emitters in an hBN–graphene van der Waals heterostructure. We show that quantum emitters in hBN can be reversibly activated and modulated by applying a bias across the device. Notably, a significant number of quantum emitters are intrinsically dark, and become optically active at non-zero voltages. To explain the results, we provide a heuristic electrostatic model of this unique behaviour. Finally, employing these devices we demonstrate a nearly-coherent source with linewidths of ~ 160 MHz. Our results enhance the potential of hBN for tunable solid state quantum emitters for the growing field of quantum information science.
Energy-Efficient Allocation for Multiple Tasks in Mobile Edge Computing
Authors: Liu Xi , Liu Jun 
Published: December 2021

Abstract
Mobile edge computing (MEC) allows a mobile device to offload tasks to the nearby server for remote execution to enhance the performance of user equipment. A major challenge of MEC is to design an efficient algorithm for task allocation. In contrast to previous work on MEC, which mainly focuses on single-task allocation for a mobile device with only one task to be completed, this paper considers a mobile device with multiple tasks or an application with multiple tasks. This assumption does not hold in real settings because a mobile device may have multiple tasks waiting to execute. We address the problem of task allocation with minimum total energy consumption considering multi-task settings in MEC, in which a mobile device has one or more tasks. We consider the binary computation offloading mode and formulate multi-task allocation as an integer programming problem that is strongly $NP$-hard. We propose an approximation algorithm and show it is a polynomial-time approximation scheme that saves the maximum energy. Therefore, our proposed algorithm achieves a tradeoff between optimality loss and time complexity. We analyze the performance of the proposed algorithm by performing extensive experiments. The results of the experiments demonstrate that our proposed approximation algorithm is capable of finding near-optimal solutions, and achieves a good balance of speed and quality.
Antibiotic treatment impaired growth of tobacco in vitro shoots is associated with oxidative injury and reduced microbiome diversity
Authors: Tamošiūnė Inga , Andriūnaitė Elena , Vinskienė Jurgita , Stanys Vidmantas , Rugienius Rytis , Baniulis Danas 
Published: December 2021

Abstract

Plant in vitro cultures initiated from surface-sterilized explants often harbor complex microbial community. Antibiotics are commonly used to decontaminate plant tissue culture or during genetic transformation, however, the effect of antibiotic treatment on the diversity of indigenous microbial population and consequences for performance of the tissue culture are not completely understood. Therefore, the aim of this study was to assess the effect of antibiotic treatment on the growth and stress level of tobacco (Nicotiana tabacum L.) in vitro shoots as well as the composition of plant-associated microbiome. The study revealed that shoot cultivation on medium supplemented with 250 mg L−1 timentin resulted in 29 ± 4% reduced biomass accumulation and 1.2–1.6 fold higher level of oxidative stress injury compared to control. Moreover, the growth properties of shoots were only partially restored after transfer to medium without antibiotic. Shoot microbiome analysis using multi-variable region-based 16S rRNA sequencing revealed abundant microbial community in the control tobacco shoots, including bacteria from 59 families, however, it was largely dominated by Mycobacteriaceae. The antibiotic treatment resulted in a decline of species richness (the number of families was reduced 4.5-fold) and increased domination by the Mycobacteriaceae family. The results imply that the diversity of plant-associated microbiome might represent a significant factor contributing to the efficient propagation of in vitro tissue culture.
Pre-clinical validation of a novel pan-cancer CAR-T cell immunotherapy against nfP2X7
Authors: Bandara Veronika , Foeng Jade , Gundsambuu Batjargal , Norton Todd , Napoli Silvana , McPeake Dylan , Tyllis Timona , Rad Elaheh Rohani , Abbott Caitlin , Mills Stuart , Tan Lih , Willet Vasiliki , Nikitaras Victoria , Johnson Adam , Coombs Justin , Oehler Martin , Ricciardelli Carmela , Cowin Allison , Bonder Claudine , Jensen Michael , Sadlon Timothy , McColl Shaun , barry simon 
Published: December 2021

Abstract
Chimeric antigen receptor (CAR)-T immunotherapy is a novel treatment that genetically modifies the patient’s own T cells to target and kill malignant cells. CAR-T cells demonstrated robust clinical activity against certain B-cell malignancies. However, identification of tumour-specific antigens expressed on multiple cancer types, especially on solid cancers, remains a major challenge. P2X purinoceptor 7 (P2X7) is an ATP gated cation channel that forms homotrimers and heterotrimers at the cell surface. When functioning normally, it controls ion transport in response to ATP. A dysfunctional version of P2X7, named nfP2X7, has been identified on cancer cells from a range of tissues, while being undetectable on healthy cells. We generated prototype nfP2X7-targeting human CAR-T cells, which demonstrated effective antigen-specific cytotoxicity against twelve solid cancer types including breast, prostate, lung, colorectal, brain and skin in vitro. In preclinical xenograft mouse models of aggressive breast and prostate cancer, CAR-T cells targeting nfP2X7 exhibited robust anti-tumour efficacy. These data indicate CAR-T cells targeting nfP2X7 have potential as a novel broad-spectrum cancer immunotherapy for solid tumours in humans.
Analysis of the General Tool Life Function of Cutting Tools by Application of the Catastrophe-Theory
Authors: Pálmai Zoltán , Kundrák János , Felhő Csaba 
Published: December 2021

Abstract
Production technology planning requires information on tool life T and its relation to cutting speed v. As the Taylor formula often cannot be linearized on an lg-lg scale, a general tool life function has been developed for describing a v-T function with a convex-concave part. Using catastrophe theory, an analogy is established between the general tool life function and the cusp catastrophe, allowing topological mapping of the general v-T function. Results were verified by machinability tests in the turning of C35 and C60 conventional and specially deoxidized C-steels during steelmaking. It was found that in the convex-concave section of this function, 2–3 cutting speeds can be selected for a given tool life, which is advantageous for harmonizing tool changes in multi-operation technology.
Assessing the Toxicological Interaction Effects of Imidacloprid, Thiamethoxam, and Chlorpyrifos on Bombus Terrestris Based on the Combination Index
Authors: Zhang Yongkui , Zeng Dongqiang , Li Lu , Hong Xiuchun , Li-Byarlay Hongmei , Luo Shudong 
Published: December 2021

Abstract
In modern agricultural production, a variety of pesticides are widely used to protect crops against pests. However, extensive residues of these pesticides in the soil, water, and pollen have negatively affected the health of nontarget organisms, especially among pollinators such as bumblebees. As an important pollinator, the bumblebee plays a vital role in agricultural production and the maintenance of ecosystem diversity. Previous research has focused on the effects of a single pesticide on pollinating insects; however, the synergistic effects of multiple agents on bumblebees have been not studied in detail. In our test, the individual and combined toxicities of chlorpyrifos, thiamethoxam, and imidacloprid to bumblebees after 48 h of oral administration were documented by the equivalent linear equation method. Our results showed that the toxicity of each single pesticide exposure, from high to low, was imidacloprid, thiamethoxam, and chlorpyrifos. All binary and ternary combinations showed synergistic or additive effects. Therefore, our research not only shows that the mixed toxicity of insecticides has a significant effect on bumblebees, but also provides scientific guidelines for assessing the safety risks to bumblebees of these three insecticide compounds. In assessing the risk to pollinating insects, the toxicity levels of laboratory experiments are much lower than the actual toxicity in the field.
Ex Vivo Culture Models of Hidradenitis Suppurativa for Defining Molecular Pathogenesis and Treatment Efficacy of Novel Drugs
Authors: Goliwas Kayla , Kashyap Mahendra P , Khan Jasim , Sinha Rajesh , Weng Zhiping , Oak Allen SW , Jin Lin , Atigadda Venkatram , Lee Madison B , Elmets Craig A , Mukhtar M Shahid , Raman Chander , Deshane Jessy , Athar Mohammad 
Published: December 2021

Abstract
Hidradenitis suppurativa (HS) is a complex inflammatory and debilitating skin disease for which no effective treatment is available currently. This is partly because of the lack of adequate human or animal models to define the pathobiology of the disease. Here, we describe the development of air-liquid (A-L) interface, liquid-submersion (L-S) and bioreactor (Bio) ex vivo skin culture models. All three ex vivo platforms were effective for culturing skin samples up to 14 days. Tissue architecture and integrity remained intact for at least 3 days for healthy skin and 14 days for HS skin. Up to day-3, no significant differences were observed in % early apoptotic cells among all three platforms. However, an increase was observed in late apoptotic/necrotic cells in HS skin at day-3 in A-L and Bio culture. These cultures efficiently support the growth of various cells populations, including keratinocytes and immune cells. Profiling inflammatory gene signatures in HS skin from these ex vivo cultures showed dynamic expression changes at day-3 and day-14. All three culture platforms are necessary to represent the inflammatory gene status of HS skin at day-0, suggesting that not all gene clusters are identically altered in each culture method. Similarly, cytokine/chemokine profiling of the supernatants from vehicle- and drug-treated ex vivo HS cultures again showed better prediction of drug efficacy against HS. Overall, development of these three culture systems collectively provides a powerful tool to uncover the pathobiology of HS progression and screen various drugs against HS.
Rapid Direct Laser Writing of Microoptical Components on A Meltable Biocompatible Gel
Authors: Radmilović Mihajlo D , Murić Branka D. , Gujić Dušan , Zarkov Boban , Nenadić Marija Z. , Pantelic Dejan V 
Published: December 2021

Abstract
Microoptical components are coming of age in a wide range of applications: lab-on-a-chip, imaging, detection... There are a large number of fabrication technologies capable of producing high quality individual components and their arrays. However, most of them require high-end and costly equipment, complex and time-consuming fabrication, harmful chemicals, resulting in expensive final products. Here we present a technology capable of producing high quality microoptical components, using low-end direct laser writing on a biocompatible, environmentally friendly hydrogel, without any waste substances. Gel is locally and controllably melted while surface tension forces shape the optical component, following the laser beam profile. Process is so quick that a single microlens is fabricated in less than a second, and can be used instantly without any further processing. The technology is neither subtractive nor additive, and the base material is simply displaced producing a smooth surface. We have been able to fabricate individual microlenses and their arrays (positive, negative, aspheric), gratings and diffractive components. The technology is tested by generating unique, difficult to counterfeit QR-codes. Turnaround time is fast and makes the technology suitable both for rapid prototyping and serial production.
Effects of Portal Preconditioning In Low-Volume Liver Remnants: Study In Rats
Authors: Ribeiro Alexandre de Abreu , Takiya Christina Maeda , Pannain Vera Nunes , Perez Mauricio Andrade , Filho Joaquim Ribeiro 
Published: December 2021

Abstract
The increase of liver surgical indications, the expansion of the margins in hepatic resections and the lack of organ donors led to the use of more split livers from cadaver and living donors and smaller liver remnants in post-operatory patients. The use of increasingly smaller grafts associated with hepatic resections broadened the spectrum for observation of small-for size syndrome, caused by significant inflammation and early hepatic fibrosis. The small-for-size syndrome is manifested clinically by prolonged cholestasis, refractory ascites and progressive hepatic dysfunction (encephalopathy and coagulopathy). In the search for mechanisms to reduce liver damage, preconditioning is presented as a possibility of protecting the low weight remnant in experimental works. Objective: Study the hepatic tissue measuring the impact of portal preconditioning in small hepatic remnant in Wistar rats Methods: Rats weighing approximately 250g were divided in 4 groups with 7 members each. Group 1, Control group requiring only collection of the material, blood laboratory analysis and liver biopsy for pathology and immunohistochemistry; Group 2, Sham, were operated with simple laparotomy, 48 hours later they were subjected to another surgery with sample collection to do blood laboratory analysis and liver biopsy for pathology and immunohistochemistry. Group 3, hepatectomy with preconditioning. In this group was made the preconditioning procedure before the resection of 70% of the liver, 48 hours later they underwent another surgery for sample collection to do blood laboratory analysis and liver biopsy for pathology and immunohistochemistry. Group 4, hepatectomy without preconditioning. In this group the members were operated with resection of 70% of the liver, 48 hours later were reoperated with sample collection to do blood laboratory analysis and liver biopsy for pathology and immunohistochemistry. We studied and compared the impacts in morphology, laboratory, histology, immunohistochemistry.Results: There was no intraoperative mortality in the model used, there was no statistically significant difference in histological and laboratory parameters between the groups with and without preconditioning, there was an increase in the expression of PCNA with statistical significance in the hepatic remnant of the group submitted to preconditioning. Conclusion: Liver preconditioning can provide an increase in cell proliferation in small volume liver remnant.
Laser Transmission Welding of Aluminum Film Coated With Heat Sealable Co-Polyester Resin With Polypropylene Films For Applications In Food &amp; Drug Packaging
Authors: Gisario Annamaria , Aversa Clizia , Barletta Massimiliano , Natali Stefano , Veniali Francesco 
Published: December 2021

Abstract
The present work deals with the high-power diode laser joining process of aluminum films coated with a polyester resin with polypropylene (PP) films. The first part of the work focused on analyzing the coating process of aluminum films with a polyester resin, using an automatic applicator. The second part of the work was focused on the analysis of the laser joining process of coated aluminum films with plastic counterparts made of PP. Different thicknesses and colors of the PP parts were tested in order to analyze the joining process under a wide range of different conditions. The experimental plan involved the study of the influence of the laser joining parameters, in particular the scanning speed and beam power, on the joints. The joints between aluminum and PP films were subsequently tested by means of tensile and peel-off tests. The results allowed the detection of the best processing conditions, stating the high potential of laser systems in the joining process of aluminum and PP films for food packaging applications.
ALF-Score++ - Transferability of a Predictive Network-Based Walkability Scoring System
Authors: Alfosool Ali M. S. , Chen Yuanzhu , Fuller Daniel 
Published: December 2021

Abstract
Walkability is an important measure with strong ties to our health. However, there are existing gaps in the literature. Our previous work proposed new approaches to address existing limitations. This paper explores new ways of applying transferability using transfer-learning. Road networks, POIs, and road-related characteristics grow/change over time. Moreover, calculating walkability for all locations in all cities is very time-consuming. Transferability enables reuse of already-learned knowledge for continued learning, reduce training time, resource consumption, training labels and improve prediction accuracy. We propose ALF-Score++, that reuses trained models to generate transferable models capable of predicting walkability score for cities not seen in the process. We trained transfer-learned models for St. John's NL and Montréal QC and used them to predict walkability scores for Kingston ON and Vancouver BC. MAE error of 13.87 units (ranging 0-100) was achieved for transfer-learning using MLP and 4.56 units for direct-training (random forest) on personalized clusters.
Amorphous WO3 thin films designed as gigahertz/terahertz dielectric lenses
Authors: Qasrawi Atef , Alrub Shatha Abu , Daragme Rana 
Published: December 2021

Abstract
Herein, tungsten oxide thin films comprising excess oxygen are treated as optical resonator suitable for gigahertz/terahertz applications. WO 3 thin films which are prepared by the thermal evaporation technique under a vacuum pressure of 10 -5 mbar are structurally, compositionally and optically evaluated. The amorphous WO 3.33 films which showed high transparency permit electronic transitions within an indirect allowed energy band gap of 3.05 eV. The band gap comprised energy band tails of width of 190 meV. Four dominant dielectric resonators centered in the infrared (IR), visible (VIS) and ultraviolet (UV) ranges of light are detected. Analysis of the optical conductivity in accordance with the Drude-Lorentz approaches have shown that the drift mobility of free holes in this amorphous layer can be as large as 5.61 cm 2 /Vs an as low as 1.59 cm 2 /Vs when exposed to IR and UV light signals, respectively. In addition, the gigahertz/terahertz cutoff frequency ( ) spectra demonstrated values in the gigahertz frequency domain when exposed to IR light. Excitations with light signals in the VIS and UV spectral ranges allow values that extends from 0.7-40.0 THz. The wide range of tunability of the WO 3 dielectric resonators nominates them as dielectric lenses suitable for optical communications.