bluuebunny/crossref_metadata_2025_split huggingface dataset:
Watershed Sediment Yield Following the 2018 Carr Fire, Whiskeytown National Recreation Area, Northern California
Authors: East Amy E. , Logan Joshua B. , Dartnell Peter , Lieber‐Kotz Oren , Cavagnaro David B. , McCoy Scott W. , Lindsay Donald N. 
Published: September 2021

AbstractWildfire risk has increased in recent decades over many regions, due to warming climate and other factors. Increased sediment export from recently burned landscapes can jeopardize downstream infrastructure and water resources, but physical landscape response to fire has not been quantified for some at‐risk areas, including much of northern California, USA. We measured sediment yield from three watersheds (13–29 km2) that drain to Whiskeytown Lake, California, within the area burned by the 2018 Carr Fire. Structure‐from‐Motion photogrammetry on aerial images combined with sonar bathymetric mapping of submerged areas indicated first‐year post‐fire sediment yields of 4,080 ± 598 t/km2 (Brandy Creek), 2,700 ± 527 t/km2 (Boulder Creek), and 305 ± 58.0 t/km2 (Whiskey Creek)—some of the first post‐fire yields measured in northern California and 64, 42, and 4.8 times greater than pre‐fire yields, respectively. These were measured during a wet year and resulted largely from rilling erosion and fluvial sediment transport, without post‐fire debris flows. Rilling preferentially developed in contact with dirt roads, aided by thin soils and exposed bedrock, and on slopes vegetated by chaparral pre‐fire. The second post‐fire year (a dry year) was characterized by fluvial reworking and delta progradation of the first‐year deposits and relatively little new sediment export. First‐year sedimentation of 111,000 m3 represented minor loss of storage capacity in Whiskeytown Lake but would be detrimental to smaller reservoirs; in general, increased sediment yields from western US watersheds as fire and extreme rainfall increase will likely pose risks to water quality and storage.
Year‐Round Measurements of Dissolved Black Carbon in Coastal Southeast Asia Aerosols: Rethinking Its Atmospheric Deposition in the Ocean
Authors: Geng Xiaofei , Zhong Guangcai , Liu Junwen , Sun Yue , Yi Xin , Bong Chui Wei , Zakaria Mohamad Pauzi , Gustafsson Örjan , Ouyang Yan , Zhang Gan 
Published: September 2021

AbstractDissolved black carbon (DBC) is an important recalcitrant fraction of marine dissolved organic matter. Riverine discharge is the largest known source of oceanic DBC; however, the significance of atmospheric deposition as a source of oceanic DBC remains poorly understood. In this study, year‐round aerosol sampling was carried out at a rural coastal site in Southeast Asia for DBC analysis using the benzene polycarboxylic acid (BPCA) method. The results revealed the uncertainty of an earlier estimate of the atmospheric deposition flux of DBC to the global ocean (FDBC), which assumed a linear correlation between DBC and water‐soluble organic carbon (WSOC). The correlation between DBC and WSOC depended on the sources of carbonaceous aerosols. The DBC/WSOC ratios were higher for the biomass burning aerosols. DBC was linearly correlated with black carbon (BC) for biomass or fossil fuel combustion aerosols. However, the DBC/BC ratios were higher for biomass burning aerosols (0.41 ± 0.22), whereas lower for fossil fuel combustion aerosols (0.04 ± 0.03). FDBC was revisited based on the relationship between DBC and BC. FDBC is primarily contributed by biomass burning aerosols and maybe previously underestimated. In this study, the DBC in aerosols had less condensed aromatic structures than the DBC present in the major rivers of the world, as shown by the BPCA compositions. This indicated that oceanic DBC sourced from atmospheric deposition was less likely to be removed by photodegradation and sedimentation, as compared to the DBC sourced from riverine discharge.
Metallographic Cooling Rate and Petrogenesis of the Recently Found Huoyanshan Iron Meteorite Shower
Authors: Wang Nian , Wang Guiqin , Zhang Ting , Gu Lixin , Zhang Chi , Hu Sen , Miao Bingkui , Lin Yangting 
Published: September 2021

AbstractThe Huoyanshan iron meteorite shower, recently found in the Gobi Desert of Hami, Xinjiang, China, has very high Ni (21.1 wt%) content and low Au (2.0 ppm), Ir (0.02 ppm), Ge (1.7 ppm), and Ga (1.1 ppm) contents, and was classified into IAB‐sLH subgroup. The iron has a finest octahedrite structure of Widmanstätten pattern (the intergrowth of kamacite [α] and taenite [γ]) with plessite matrix, and euhedral schreibersite (Sch) crystals exclusively enclosed in kamacite bands. The textural features suggest the following formation process: γ→γ + Sch→γ + Sch + α, and then γ→α2 + γ. The metallographic cooling rate of Huoyanshan iron was determined to be 3–50°C/Myr using both the taenite Ni profile‐matching and taenite central Ni content methods, with the bandwidths corrected for crystallographic orientation by electron backscatter diffraction (EBSD). The cooling rate of Huoyanshan is consistent with other sLH irons and confirms the slow cooling history of the IAB low‐Au subgroups. These slow cooling rates require immediate re‐accretion with a thick brecciated fragments layer in the parent body after the impact melting event. The depleted but unfractionated Re, Os, Ir, Ru, and Pt and the enriched Pd and Au abundances of Huoyanshan iron and other sLH subgroup irons show complementary feature to that of refractory metal nuggets in Ca‐, Al‐rich inclusions (CAIs), which could be explained by extracting the metallic Fe‐Ni with HSE predominantly remained in CAIs from a CAI‐bearing asteroid. The very high Ni content of sLH subgroup and its Mo isotope evidence (Worsham et al., 2017, https://doi.org/10.1016/j.epsl.2017.02.044) suggest it has an oxidized parent body with non‐carbonaceous chondrite‐like precursor composition. We propose that the sLH subgroup was produced by impact melting of a LL like and CAI‐bearing asteroid, followed by fast burying of thick and porous silicate breccia.
Decreasing Groundwater Supply Can Exacerbate Lake Warming and Trigger Algal Blooms
Authors: Safaie Ammar , Litchman Elena , Phanikumar Mantha S. 
Published: September 2021

AbstractAs groundwater depletion becomes a global phenomenon, inland lake ecosystems are being impacted by decreasing groundwater supply. While the current trend of rapid surface warming of inland lakes continues, the deep waters can resist changes, depending on the nature of surface water — groundwater interactions. However, the effects of these interactions on lake processes are not fully understood. Here we investigate the role of groundwater on coupled biophysical processes in a deep, dimictic, groundwater‐fed lake using mechanistic models combined with data from field observations. Although excess nutrient inputs are the most commonly cited reason for algal blooms, here we show that algal blooms in inland lakes can also appear due to a decreasing groundwater supply while all other factors remain the same. Results indicate that decreasing groundwater supply to lakes leads to elevated hypolimnetic temperatures, enhanced algal growth rates and algal blooms, and oxygen depletion, thus exacerbating the negative effects of surface warming. Our work suggests that globally declining groundwater supplies may have a significant negative effect on water quality of inland lakes by accelerating water column warming and stimulating algal growth, especially when the groundwater contribution to the lake system is significant compared to riverine discharge. The work provides insights for management efforts to improve the resilience of groundwater‐dependent ecosystems in the face of external stressors.
Porphyromonas gingivalis infection promotes mitochondrial dysfunction through Drp1-dependent mitochondrial fission in endothelial cells
Authors: Xu Tong , Dong Qin , Luo Yuxiao , Liu Yanqing , Gao Liang , Pan Yaping , Zhang Dongmei 
Published: September 2021

AbstractPorphyromonas gingivalis (P. gingivalis), a key pathogen in periodontitis, has been shown to accelerate the progression of atherosclerosis (AS). However, the definite mechanisms remain elusive. Emerging evidence supports an association between mitochondrial dysfunction and AS. In our study, the impact of P. gingivalis on mitochondrial dysfunction and the potential mechanism were investigated. The mitochondrial morphology of EA.hy926 cells infected with P. gingivalis was assessed by transmission electron microscopy, mitochondrial staining, and quantitative analysis of the mitochondrial network. Fluorescence staining and flow cytometry analysis were performed to determine mitochondrial reactive oxygen species (mtROS) and mitochondrial membrane potential (MMP) levels. Cellular ATP production was examined by a luminescence assay kit. The expression of key fusion and fission proteins was evaluated by western blot and immunofluorescence. Mdivi-1, a specific Drp1 inhibitor, was used to elucidate the role of Drp1 in mitochondrial dysfunction. Our findings showed that P. gingivalis infection induced mitochondrial fragmentation, increased the mtROS levels, and decreased the MMP and ATP concentration in vascular endothelial cells. We observed upregulation of Drp1 (Ser616) phosphorylation and translocation of Drp1 to mitochondria. Mdivi-1 blocked the mitochondrial fragmentation and dysfunction induced by P. gingivalis. Collectively, these results revealed that P. gingivalis infection promoted mitochondrial fragmentation and dysfunction, which was dependent on Drp1. Mitochondrial dysfunction may represent the mechanism by which P. gingivalis exacerbates atherosclerotic lesions.
miR-98-5p plays a critical role in depression and antidepressant effect of ketamine
Authors: Huang Chaoli , Wang Yuanyuan , Wu Zifeng , Xu Jiali , Zhou Ling , Wang Di , Yang Ling , Zhu Bin , Chen Guiquan , Liu Cunming , Yang Chun 
Published: September 2021

AbstractKetamine has been demonstrated to be a rapid-onset and long-lasting antidepressant, but its underlying molecular mechanisms remain unclear. Recent studies have emerged microRNAs as important modulators for depression treatment. In this study, we report that miR-98-5p is downregulated in the prefrontal cortex and hippocampus of mice subjected to chronic social stress, while overexpressing it by its agonist alleviates depression-like behaviors. More importantly, we demonstrate that miR-98-5p is upregulated by ketamine administration, while inhibition of it by its antagonist blocks the antidepressant effect of ketamine. Our data implicate a novel molecular mechanism underlying the antidepressant effect of ketamine, and that therapeutic strategies targeting miR-98-5p could exert beneficial effects for depression treatment.
Endotoxin stabilizes protein arginine methyltransferase 4 (PRMT4) protein triggering death of lung epithelia
Authors: Lai Yandong , Li Xiuying , Li Tiao , Nyunoya Toru , Chen Kong , Kitsios Georgios D. , Nouraie Seyed Mehdi , Zhang Yingze , McVerry Bryan J. , Lee Janet S. , Mallampalli Rama K. , Zou Chunbin 
Published: September 2021

AbstractLung epithelial cell death is a prominent feature of acute lung injury and acute respiratory distress syndrome (ALI/ARDS), which results from severe pulmonary infection leading to respiratory failure. Multiple mechanisms are believed to contribute to the death of epithelia; however, limited data propose a role for epigenetic modifiers. In this study, we report that a chromatin modulator protein arginineN-methyltransferase 4/coactivator-associated arginine methyltransferase 1 (PRMT4/CARM1) is elevated in human lung tissues with pneumonia and in experimental lung injury models. Here PRMT4 is normally targeted for its degradation by an E3 ubiquitin ligase, SCFFBXO9, that interacts with PRMT4 via a phosphodegron to ubiquitinate the chromatin modulator at K228 leading to its proteasomal degradation. Bacterial-derived endotoxin reduced levels of SCFFBXO9thus increasing PRMT4 cellular concentrations linked to epithelial cell death. Elevated PRMT4 protein caused substantial epithelial cell death via caspase 3-mediated cell death signaling, and depletion of PRMT4 abolished LPS-mediated epithelial cell death both in cellular and murine injury models. These findings implicate a unique molecular interaction between SCFFBXO9and PRMT4 and its regulation by endotoxin that impacts the life span of lung epithelia, which may play a key role in the pathobiology of tissue injury observed during critical respiratory illness.
NudCL2 is an autophagy receptor that mediates selective autophagic degradation of CP110 at mother centrioles to promote ciliogenesis
Authors: Liu Min , Zhang Wen , Li Min , Feng Jiaxing , Kuang Wenjun , Chen Xiying , Yang Feng , Sun Qiang , Xu Zhangqi , Hua Jianfeng , Yang Chunxia , Liu Wei , Shu Qiang , Yang Yuehong , Zhou Tianhua , Xie Shanshan 
Published: September 2021

AbstractPrimary cilia extending from mother centrioles are essential for vertebrate development and homeostasis maintenance. Centriolar coiled-coil protein 110 (CP110) has been reported to suppress ciliogenesis initiation by capping the distal ends of mother centrioles. However, the mechanism underlying the specific degradation of mother centriole-capping CP110 to promote cilia initiation remains unknown. Here, we find that autophagy is crucial for CP110 degradation at mother centrioles after serum starvation in MEF cells. We further identify NudC-like protein 2 (NudCL2) as a novel selective autophagy receptor at mother centrioles, which contains an LC3-interacting region (LIR) motif mediating the association of CP110 and the autophagosome marker LC3. Knockout of NudCL2 induces defects in the removal of CP110 from mother centrioles and ciliogenesis, which are rescued by wild-type NudCL2 but not its LIR motif mutant. Knockdown of CP110 significantly attenuates ciliogenesis defects in NudCL2-deficient cells. In addition, NudCL2 morphants exhibit ciliation-related phenotypes in zebrafish, which are reversed by wild-type NudCL2, but not its LIR motif mutant. Importantly, CP110 depletion significantly reverses these ciliary phenotypes in NudCL2 morphants. Taken together, our data suggest that NudCL2 functions as an autophagy receptor mediating the selective degradation of mother centriole-capping CP110 to promote ciliogenesis, which is indispensable for embryo development in vertebrates.
Long-term treatment with senolytic drugs Dasatinib and Quercetin ameliorates age-dependent intervertebral disc degeneration in mice
Authors: Novais Emanuel J. , Tran Victoria A. , Johnston Shira N. , Darris Kayla R. , Roupas Alex J. , Sessions Garrett A. , Shapiro Irving M. , Diekman Brian O. , Risbud Makarand V. 
Published: September 2021

AbstractIntervertebral disc degeneration is highly prevalent within the elderly population and is a leading cause of chronic back pain and disability. Due to the link between disc degeneration and senescence, we explored the ability of the Dasatinib and Quercetin drug combination (D + Q) to prevent an age-dependent progression of disc degeneration in mice. We treated C57BL/6 mice beginning at 6, 14, and 18 months of age, and analyzed them at 23 months of age. Interestingly, 6- and 14-month D + Q cohorts show lower incidences of degeneration, and the treatment results in a significant decrease in senescence markers p16INK4a, p19ARF, and SASP molecules IL-6 and MMP13. Treatment also preserves cell viability, phenotype, and matrix content. Although transcriptomic analysis shows disc compartment-specific effects of the treatment, cell death and cytokine response pathways are commonly modulated across tissue types. Results suggest that senolytics may provide an attractive strategy to mitigating age-dependent disc degeneration.
Longitudinal characterization of multispecies microbial populations recovered from spaceflight potable water
Authors: Yang Jiseon , Barrila Jennifer , Mark Ott C. , King Olivia , Bruce Rebekah , McLean Robert J. C. , Nickerson Cheryl A. 
Published: September 2021

AbstractWhile sequencing technologies have revolutionized our knowledge of microbial diversity, little is known about the dynamic emergent phenotypes that arise within the context of mixed-species populations, which are not fully predicted using sequencing technologies alone. The International Space Station (ISS) is an isolated, closed human habitat that can be harnessed for cross-sectional and longitudinal functional microbiome studies. Using NASA-archived microbial isolates collected from the ISS potable water system over several years, we profiled five phenotypes: antibiotic resistance, metabolism, hemolysis, and biofilm structure/composition of individual or multispecies communities, which represent characteristics that could negatively impact astronaut health and life-support systems. Data revealed a temporal dependence on interactive behaviors, suggesting possible microbial adaptation over time within the ecosystem. This study represents one of the most extensive phenotypic characterization of ISS potable water microbiota with implications for microbial risk assessments of water systems in built environments in space and on Earth.
Constructing covalent organic nanoarchitectures molecule by molecule via scanning probe manipulation
Authors: Zhong Qigang , Ihle Alexander , Ahles Sebastian , Wegner Hermann A. , Schirmeisen Andre , Ebeling Daniel 
Published: September 2021

AbstractConstructing low-dimensional covalent assemblies with tailored size and connectivity is challenging yet often key for applications in molecular electronics where optical and electronic properties of the quantum materials are highly structure dependent. We present a versatile approach for building such structures block by block on bilayer sodium chloride (NaCl) films on Cu(111) with the tip of an atomic force microscope, while tracking the structural changes with single-bond resolution. Covalent homo-dimers in cis and trans configurations and homo-/hetero-trimers were selectively synthesized by a sequence of dehalogenation, translational manipulation and intermolecular coupling of halogenated precursors. Further demonstrations of structural build-up include complex bonding motifs, like carbon–iodine–carbon bonds and fused carbon pentagons. This work paves the way for synthesizing elusive covalent nanoarchitectures, studying structural modifications and revealing pathways of intermolecular reactions.
Radiomics feature stability of open-source software evaluated on apparent diffusion coefficient maps in head and neck cancer
Authors: Korte James C. , Cardenas Carlos , Hardcastle Nicholas , Kron Tomas , Wang Jihong , Bahig Houda , Elgohari Baher , Ger Rachel , Court Laurence , Fuller Clifton D. , Ng Sweet Ping 
Published: September 2021

AbstractRadiomics is a promising technique for discovering image based biomarkers of therapy response in cancer. Reproducibility of radiomics features is a known issue that is addressed by the image biomarker standardisation initiative (IBSI), but it remains challenging to interpret previously published radiomics signatures. This study investigates the reproducibility of radiomics features calculated with two widely used radiomics software packages (IBEX, MaZda) in comparison to an IBSI compliant software package (PyRadiomics). Intensity histogram, shape and textural features were extracted from 334 diffusion weighted magnetic resonance images of 59 head and neck cancer (HNC) patients from the PREDICT-HN observational radiotherapy study. Based on name and linear correlation, PyRadiomics shares 83 features with IBEX and 49 features with MaZda, a sub-set of well correlated features are considered reproducible (IBEX: 15 features, MaZda: 18 features). We explore the impact of including non-reproducible radiomics features in a HNC radiotherapy response model. It is possible to classify equivalent patient groups using radiomic features from either software, but only when restricting the model to reliable features using a correlation threshold method. This is relevant for clinical biomarker validation trials as it provides a framework to assess the reproducibility of reported radiomic signatures from existing trials.
Ascorbic acid as an adjunctive therapy in critically ill patients with COVID-19: a propensity score matched study
Authors: Al Sulaiman Khalid , Aljuhani Ohoud , Saleh Khalid Bin , Badreldin Hisham A. , Al Harthi Abdullah , Alenazi Mohammed , Alharbi Aisha , Algarni Rahmah , Al Harbi Shmeylan , Alhammad Abdullah M. , Vishwakarma Ramesh , Aldekhyl Sarah 
Published: September 2021

AbstractAscorbic acid represents an appealing option for clinicians to utilize in the context of the global COVID-19 pandemic due to its proposed clinical efficacy, relative safety, and low cost. The aim of this study was to evaluate the efficacy and safety of using ascorbic acid in supplemental doses as adjunctive therapy for patients critically ill with COVID-19. This was a two-center, non-interventional, retrospective cohort study. All critically ill adult patients admitted to ICU with a confirmed COVID-19 diagnosis between March 1st and December 31st, 2020, were included in the final analysis. The study was conducted at two large governmental tertiary hospitals in Saudi Arabia. The purpose was to investigate the clinical outcomes of low-dose ascorbic acid as adjunctive therapy in COVID-19 after propensity score matching using baseline severity scores, systematic use of corticosteroids, and study centers. A number of 739 patients were included in this study, among whom 296 patients were included after propensity score matching. There was no association between the administration of ascorbic acid and in-hospital mortality or the 30-day mortality [OR (95% CI) 0.77 (0.47, 1.23), p value = 0.27 and OR (95% CI) 0.73 (0.43, 1.20), p value = 0.21, respectively]. Using ascorbic acid was associated with a lower incidence of thrombosis compared with the non-ascorbic-acid group [6.1% vs. 13% respectively; OR (95% CI) 0.42 (0.184, 0.937), p value = 0.03]. Low dose of ascorbic acid as an adjunctive therapy in COVID-19 critically ill patients was not associated with mortality benefits, but it was associated with a lower incidence of thrombosis. Further studies are required to confirm these findings.
Raspberry ketone diet supplement reduces attraction of sterile male Queensland fruit fly to cuelure by altering expression of chemoreceptor genes
Authors: Khan Mohammed Abul Monjur , Deshpande Nandan P. , Shuttleworth Lucas A. , Osborne Terry , Collins Damian , Wilkins Marc R. , Gurr Geoff M. , Reynolds Olivia L. 
Published: September 2021

AbstractSterile male Queensland fruit fly, Bactrocera tryoni (Froggatt), fed as immature adults on the plant compound raspberry ketone (RK), show a reduced attraction to cuelure, a synthetic analogue of RK used as an attractant in Male Annihilation Technique. We hypothesized the reduced attraction of RK-fed adult males to cuelure may be a consequence of altered expression of chemoreception genes. A Y-tube olfactometer assay with RK-fed and RK-unfed sterile B. tryoni males tested the subsequent behavioural response to cuelure. Behavioral assays confirmed a significant decrease in attraction of RK-fed sterile males to cuelure. RK-fed, non-responders (to cue-lure) and RK-unfed, responders (to cue-lure) males were sampled and gene expression compared by de novo RNA-seq analysis. A total of 269 genes in fly heads were differentially expressed between replicated groups of RK-fed, cuelure non-responders and RK-unfed, cuelure responders. Among them, 218 genes including 4 chemoreceptor genes were up regulated and 51 genes were down regulated in RK-fed, cuelure non-responders. De novo assembly generated many genes with unknown functions and no significant BLAST hits to homologues in other species. The enriched and suppressed genes reported here, shed light on the transcriptional changes that affect the dynamics of insect responses to chemical stimuli.