bluuebunny/crossref_metadata_2025_split huggingface dataset:
Integrating spatial, phylogenetic, and threat assessment data from frogs and lizards to identify areas for conservation priorities in Peninsular Malaysia
Authors: Chan Kin Onn , Grismer L. Lee 
Published: April 2021

AbstractMalaysia is recognized as a megadiverse country and biodiversity hotspot, which necessitates sufficient levels of habitat protection and effective conservation management. However, conservation planning in Malaysia has hitherto relied largely on species distribution data without taking into account the rich evolutionary history of taxa. This represents the first study that integrates spatial and evolutionary approaches to identify important centers of diversity, endemism, and bioregionalization that can be earmarked for conservation priorities in Peninsular Malaysia. Using georeferenced species occurrences, comprehensive phylogenies, and threat assessments of frogs and lizards, we employed a spatial phylogenetics framework that incorporates various diversity metrics including weighted endemism, phylogenetic diversity, phylogenetic endemism, and evolutionary distinctiveness and global endangerment. Ten areas of high conservation value were identified via the intersection of these metrics—northern Perlis, Langkawi Geopark, southern Bintang range, Cameron Highlands, Fraser’s Hill, Benom-Krau complex, Selangor-Genting complex, Endau-Rompin National Park, Seribuat Archipelago (Tioman and Pemanggil Islands), and southern Johor. Of these, Cameron Highlands requires the highest conservation priority based on severe environmental degradation, inadequately protected areas, and high numbers of endangered and evolutionary distinct species. Other areas, especially in the northwestern (states of Kedah and Penang) and northeastern regions (states of Kelantan) were not only identified as areas of high conservation value but also areas of biogeographic importance. Taken together, frogs and lizards demonstrate distinct east-west and north-south patterns of bioregionalization that are largely modulated by mountain ranges.Article Impact StatementThe first study to use a spatial phylogenetic approach to identify areas for conservation priorities in Malaysia
An artificial cell containing cyanobacteria for endosymbiosis mimicking
Authors: Yang Boyu , Li Shubin , Mu Wei , Wang Zhao , Han Xiaojun 
Published: April 2021

AbstractThe bottom-up constructed artificial cells help to understand the cell working mechanism and provide the evolution clues for organisms. Cyanobacteria are believed to be the ancestors of chloroplasts according to endosymbiosis theory. Herein we demonstrate an artificial cell containing cyanobacteria to mimic endosymbiosis phenomenon. The cyanobacteria sustainably produce glucose molecules by converting light energy into chemical energy. Two downstream “metabolic” pathways starting from glucose molecules are investigated. One involves enzyme cascade reaction to produce H2O2(assisted by glucose oxidase) first, followed by converting Amplex red to resorufin (assisted by horseradish peroxidase). The more biological one involves nicotinamide adenine dinucleotide (NADH) production in the presence of NAD+and glucose dehydrogenase. Further, NADH molecules are oxidized into NAD+by pyruvate catalyzed by lactate dehydrogenase, meanwhile, lactate is obtained. Therefore, the sustainable cascade cycling of NADH/NAD+is built. The artificial cells built here simulate the endosymbiosis phenomenon, meanwhile pave the way for investigating more complicated sustainable energy supplied metabolism inside artificial cells.
IKKβ signaling mediates metabolic changes in the hypothalamus of a Huntington’s disease mouse model
Authors: Soylu-Kucharz Rana , Khoshnan Ali , Petersén Åsa 
Published: April 2021

AbstractBackgroundHuntington’s disease (HD) is a neurodegenerative disorder caused by a CAG repeat expansion in the huntingtin (HTT) gene. Metabolic changes are associated with HD progression, and underlying mechanisms are not fully known. As the IKKβ/NF-κB pathway is an essential regulator of metabolism, we investigated the involvement of IKKβ, the upstream activator of NF-κB in hypothalamus-specific HD metabolic changes.MethodsUsing viral vectors, we expressed amyloidogenic N-terminal fragments of mutant HTT (mHTT) fragments in the hypothalamus of mice without IKKβ in the CNS (IKKβ-/-) and control mice (IKKβ+/+). We assessed effects on body weight, metabolic hormones, and hypothalamic neuropathology.ResultsHypothalamic expression of mHTT led to an obese phenotype only in female mice. CNS-specific inactivation of IKKβ prohibited weight gain in females, which was independent of neuroprotection and microglial activation.ConclusionsThe expression of mHTT in the hypothalamus causes metabolic imbalance in a sex-specific fashion, and central inhibition of the IKKβ pathway attenuates the obese phenotype.
Developing a Petri dish test to detect resistance to key herbicides inLolium rigidum
Authors: Perez Martina Badano , Beckie Hugh J , Cawthray Gregory R , Goggin Danica E , Busi Roberto 
Published: April 2021

AbstractOverreliance on herbicides for weed control is conducive to the evolution of herbicide resistance. Annual ryegrass (Lolium rigidumGaud.) is a species that is prone to evolve resistance to a wide range of herbicide modes of action. Rapid detection of herbicide-resistant weed populations in the field can aid farmers to optimize the use of herbicides for their control. The feasibility of a portable agar-based test to rapidly and reliably detect annual ryegrass resistance to key herbicides such as clethodim, glyphosate, pyroxasulfone and trifluralin on-farm was investigated. The three research phases of this study show that: a) easy-to-interpret results are obtained with non-dormant seed from well-characterised susceptible and resistant populations, and resistance is detected as effectively as with traditional dose-response pot-based resistance assays. However, the test may not be suitable for portable use on-farm because of b) the low stability of some herbicides such as trifluralin and clethodim in agar and c) the tendency of seed dormancy in freshly-harvested seeds to confound the results. The agar-based test is best used as a research tool as a complement to confirm results obtained in traditional pot-based resistance screenings. Comprehensive agar test and / or whole-plant resistance tests by herbicide application at the recommended label rate (whole plants grown in pots) are the current benchmark for proactive in- and off-season resistance testing and should be promoted more widely to allow early detection of resistance, optimization of herbicide technology use and deploy appropriate weed management interventions.
Missing Value Imputation using XGboost for Label-Free Mass Spectrometry-Based Proteomics Data
Authors: Song Jian , Yu Changbin 
Published: April 2021

AbstractThe label-free mass spectrometry-based proteomics data inevitably suffer from the problem of missing values. The existence of missing values prevents the downstream analyses which need a complete data matrix. Our motivation is to introduce the state-of-art machine learning algorithm XGboost to realize a method of imputation which can improve the accuracy of imputation. But in practical, XGboost has many parameters need to be tuned to deliver on its potential high performance. Although cross validation may find the best parameters, it is much time-consuming. Alternatively, we empirically determined the parameters to two kinds of base learners of XGboost. To explore the robustness and performance of XGboost based imputation with predetermined parameters, we conducted tests on three benchmark datasets. As a comparative, six common imputation methods were also experimented in terms of normalized root mean squared error and Pearson correlation coefficient. The comparative experimental results indicated that the XGboost based imputation method using the linear base learner is competitive to or out-performs its competitors, including the random forest based imputation, by achieving smaller imputation errors and better structure preservation under the empirical parameters for the three benchmark datasets.
Glial contribution to cyclodextrin-mediated reversal of cholesterol accumulation in murine NPC1-deficient neuronsin vivo
Authors: Barthelemy Amélie , Demais Valérie , Stancu Izabela-Cristina , Vasile Eugeniu , Houben Tom , Reber Michael , Pallottini Valentina , Perraut Martine , Reibel Sophie , Pfrieger Frank W. 
Published: April 2021

AbstractNiemann-Pick type C (NPC) disease is a rare and fatal lysosomal storage disorder presenting severe neurovisceral symptoms. Disease-causing mutations in genes encoding eitherNPC1orNPC2protein provoke accumulation of cholesterol and other lipids in specific structures of the endosomal-lysosomal system and degeneration of specific cells, notably neurons in the central nervous system (CNS). 2-hydroxypropyl-beta-cyclodextrin (CD) emerged as potential therapeutic approach based on animal studies and clinical data, but the mechanism of action on neurons has remained unclear. To address this topicin vivo, we took advantage of the retina as highly accessible part of the (CNS) and intravitreal injections as mode of drug administration. We find that CD enters the endosomal-lysosomal system of neurons and enables the release of lipid-laden lamellar inclusions, which are then removed from the extracellular space by specific types of glial cells. Thus, CD triggers a concerted action of neurons and glial cells to restore lipid homeostasis in the central nervous system.
Dual Dean entrainment with volume ratio modulation for efficient co-encapsulation: Extreme single-cell indexing
Authors: Harrington Jack , Esteban Luis Blay , Butement Jonathan , Vallejo Andres F. , Lane Simon I. R. , Sheth Bhavwanti , Jongen Maaike S. A. , Parker Rachel , Stumpf Patrick S. , Smith Rosanna C. G. , MacArthur Ben D. , Rose-Zerilli Matthew J. J. , Polak Marta E. , Underwood Tim , West Jonathan 
Published: April 2021

AbstractThe future of single cell diversity screens involves ever-larger sample sizes, dictating the need for higher throughput methods with low analytical noise to accurately describe the nature of the cellular system. Current approaches are limited by the Poisson statistic, requiring dilute cell suspensions and associated losses in throughput. In this contribution, we apply Dean entrainment to both cell and bead inputs, defining different volume packets to effect efficient co-encapsulation. Volume ratio scaling was explored to identify optimal conditions. This enabled the co-encapsulation of single cells with reporter beads at rates of ~1 million cells/hour, while increasing assay signal-to-noise with cell multiplet rates of ~2.5% and capturing ~70% of cells. The method, called Pirouette-seq, extends our capacity to investigate biological systems.TOC AbstractPirouette-seq involves cell and reporter bead inertial ordering for efficient co-encapsulation, achieving a throughput of 1 million cells/hour, a 2.5% multiplet rate and a 70% cell capture efficiency.
An investigation of demand and exchange rate shocks in the tourism sector
Authors: Bozkurt Kurtulus , Armutçuoğlu Tekin Hatice , Can Ergün Zeliha 
Published: April 2021

PurposeThis study aims to measure the relationship between demand and exchange rate shocks in the tourism industry.Design/methodology/approachA panel data set is constructed covering the period between 1995 and 2017, and the data set includes the top 26 countries that host 10 million tourists and above in the world as of 2017. The standard errors of the series are used as an indicator of shocks. First, the cross-sectional dependency, stationarity and the homogeneity of the series are examined; second, a panel cointegration analysis is implemented; third, long-term panel cointegration coefficients are analyzed with Dynamic Common Correlated Effects (DCCE) approach; and, finally, Dumitrescu and Hurlin’s (2012) Granger non-causality test is used to detect the causality.FindingsThe preliminary analyses show that the variables are cross-sectional dependent and heterogeneous and are stationary in their first difference; hence, the effects of the shocks are temporary. On the other hand, as a result of the panel cointegration analysis, it is found that both series are cointegrated over the long-term. However, the long-term coefficients estimated with the DCCE approach are found not to be statistically significant. Finally, as a result of the Dumitrescu and Hurlin’s (2012) Granger non-causality test, it is concluded that there is a causality running from exchange rate shocks to demand shocks.Originality/valueTo the best of the authors’ knowledge, the cointegration between the tourism demand shocks and exchange rates shocks has not been investigated before, and therefore, this study is considered to be a pioneering study that will contribute to the literature.
The experience of regret in small business failure: who’s to blame?
Authors: Quach Sara , Weaven Scott K. , Thaichon Park , Grace Debra , Frazer Lorelle , Brown James R. 
Published: April 2021

PurposeFramed within the theoretical domain of attribution theory, this study aims to investigate the antecedents of experienced regret following an entrepreneur’s business failure (defined as firm discontinuance, closure or bankruptcy) and the impact of regret on personal well-being.Design/methodology/approachThe population of interest was business owners whose businesses had failed within the past five years. The data was collected from 319 failed entrepreneurs using an online survey. Structural equation modelling was used to test the hypotheses presented in this study.FindingsExternal attribution, including economic uncertainty and contract restrictions, was positively related to feelings of regret. Considering internal attribution, due diligence had a positive effect on regret whereas customer relationship development ability can reduce feelings of regret. Moreover, prevention-focused entrepreneurs were likely to experience higher levels of regret when engaging in extensive consideration in using information. Finally, regret had a detrimental effect on the entrepreneurs’ well-being.Research limitations/implicationsThe research provides fresh perspectives on experienced regret, a relatively unexplored emotion in the entrepreneurship literature. In the context of small business operations, the locus of attribution (associated with business failure) is the key influence on learning following failed business attempts.Practical implicationsThis study extends current knowledge of regret in the context of entrepreneurial failure, which has a significant catalytic effect on employment and entrepreneurial mobility.Originality/valueThis research sheds light on how emotional responses are derived from an entrepreneur’s self-assessment of their performance and attribution of blame for failure.
Market reaction to the COVID-19 pandemic: evidence from emerging markets
Authors: Harjoto Maretno Agus , Rossi Fabrizio 
Published: April 2021

PurposeThis study examines the market reaction to the World Health Organization (WHO) announcement of the novel coronavirus disease 2019 (COVID-19) as a global pandemic on the emerging equity markets and compares the reaction with developed markets. This study also compares the market reactions to the COVID-19 pandemic with the market reactions to the 2008 global financial crisis.Design/methodology/approachUsing the Morgan Stanley Capital International daily stock indices data and the Carhart and the GARCH(1,1) models for an event study, the authors examine the cumulative abnormal returns during 30 and 10 trading days and the extended 60 days before and after the WHO pandemic announcement. It also compares the market reactions during the COVID-19 pandemic with the reactions to the Lehman Brothers' bankruptcy announcement during the 2008 global financial crisis.FindingsThis study finds that the COVID-19 pandemic had a significantly greater negative impact to the stock markets in emerging countries than in the developed countries. The negative impact on the emerging markets is more pronounced for firms with small market capitalizations and for growth stocks. The negative impact of the COVID-19 pandemic is stronger in the energy and financial sectors in both emerging and developed markets. The positive impact of the COVID-19 pandemic occurred in healthcare and telecommunications for the emerging markets and information technology for the developed markets. This study also finds that the equity markets in both emerging and developed countries recovered faster from the COVID-19 pandemic relative to the 2008 global financial crisis.Social implicationsInvestors' desire to diversify their risks across different countries and sectors in the emerging markets could bring superior returns. The diversification strategies bring critical financial supports to forestall the contagion of COVID-19, to protect lives, and to save the emerging economies, especially for those financially constrained countries that are facing twin health and economic shocks by channeling their investments to countries with weak healthcare systems.Originality/valueThis study extends the literature that examines market reactions to stock market shocks by examining the market reactions to the COVID-19 outbreak on the emerging and developed equity markets across different market capitalizations, valuation and sectors. This study also finds that the markets recovered quicker from the COVID-19 pandemic announcement than during the 2008 global financial crisis.
Identifying occupational therapy outcome measures supportive of recovery-orientated mental health services in Ireland
Authors: Kearns Róisín , Salmon Nancy , Cahill Mairead , Egan Eithne 
Published: April 2021

Purpose
No occupational therapy outcome measures have been designed specifically for recovery-orientated services.This paper aims to identify occupational therapy outcome measures relevant to mental health practice and assess them against recovery principles adopted by Irish Mental Health Services.

Design/methodology/approach
A narrative review methodology was used to appraise outcome measures against CHIME recovery principles.

Findings
A systematic search across 13 databases identified eight well-established outcome measures commonly used within occupational therapy mental health literature. The included outcome measures were appraised using a recovery alignment tool.

Practical implications
All outcome measures connected to some recovery processes. Those using semi-structured interview formats and notably the Canadian Occupational Performance Measure (COPM) had the strongest alignment to recovery processes.

Originality/value
This is the first known review which provides some validation that the included outcome measures support recovery processes, yet the measures rely heavily on therapist’s skills for processes to be facilitated. It recommends that ways to better support the process of partnership in occupational therapy mental health outcome measures be explored and further research be undertaken.
Developing brand advocacy through brand activities on Facebook
Authors: Choi Youngtae , Kroff Michael W. , Kim Junga 
Published: April 2021

Purpose
This paper aims to investigate how brands’ social media activities (credible content delivery, co-creation and responsiveness) impact brand advocacy. The paper also examines the influence of brand advocacy on purchase intentions of brand advocates and the moderating effect of the amount of time spent on Facebook on the relationship between brand advocacy and purchase intentions. Finally, the moderating effect of brand type (goods vs services) on the relationship between brands’ activities and brand advocacy is explored.

Design/methodology/approach
US adults who use Facebook as their primary social media platform participated in a survey via Amazon Mechanical Turk. Structural equation modeling was used to test the proposed hypotheses.

Findings
The three brand activities are positively related to users’ advocacy of brands toward other users. Brand advocacy also positively influences purchase intentions of brand advocates. The amount of time spent on Facebook moderates the relationship between brand advocacy and purchase intentions. The brand type moderates the relationship between co-creation and brand advocacy.

Originality/value
This study differs from the existing research, which has typically used a user-oriented perspective (e.g. impact of user motivations) to explain brand advocacy and has not considered the outcome of brand advocacy on the brand advocates’ purchase intentions.
Real cost of flex-offices: discourse and reality
Authors: Nappi Ingrid , Eddial Hajar 
Published: April 2021

PurposeThis paper aims to draw on the field of real estate management to explore the cost of flex-offices as well as the corporate discourse toward it and to identify the possible gap between real estate directors' discourse and the real cost of flex-offices.Design/methodology/approachThis paper follows the grounded theory approach. Three studies were conducted: 16 semi-structured interviews with real estate actors, categorization of internal corporate documents and a thorough content analysis of the press discourse.FindingsThere is a positive vision as well as a very optimistic outlook on the flex-office on the part of real estate actors and the press. This research shows that reality does not reflect the discourse as 12 hidden costs identified are not currently factored into costs when setting up a flex-office, which can distort the discourse and efficiency. It will be interesting to measure those hidden costs in future research so as to have an objective discourse and a better cost estimate when deploying this kind of workspace.Originality/valueContradict the widespread discourse about flex-offices by highlighting the hidden costs that should be taken into consideration to allow managers to make accurate estimates. Although these hidden costs may seem ancillary at first sight, they might have an impact on efficiency.
Corporate real estate management and companies’ success: empirical evidence for a conceptual framework
Authors: Pfnuer Andreas , Seger Julian , Appel-Meulenbroek Rianne 
Published: April 2021

PurposeThe purpose of this study is to explain the contribution of Corporate Real Estate Management (CREM) to corporate success and to substantiate it empirically. However, no empirically tested holistic concept classifies and explains the different success contributions of CREM in their mechanisms of action and organisational levels.Design/methodology/approachThis study develops a holistic two-dimensional model from existing literature to explain the relationship between CREM decisions and business success, and then tests it empirically using multidimensional data scaling from a telephone company survey (CATI) of 59 CREM managers sampled from the 200 largest German companies.FindingsThe created theoretical model holistically explains CREM success and existence as part of a non-property company, with specific performance drivers on specific organisational levels. The empirical data confirm that both dimensions of the model and, thus the measurement concept for modelling the CREM contribution to business success is robust across sectors and company/portfolio size in Germany.Originality/valueThe empirical confirmation of the conceptual model of CREM success provides novel support for the institutionalisation of the CREM function in companies and the holistic classification of different CREM research directions.