bluuebunny/crossref_metadata_2025_split huggingface dataset:
Multi-omics Uncover Neonatal Cecal Cell Development Potentials
Authors: Chen Liang , Meng Qingshi , Li Shen , Jiang Yue , Zhang Cong , Han Hui , Tang Shanlong , Zhong Ruqing , Tang Xiangfang , Zhang Sheng , Feng Xiaohui , Zhou Yexun , Zhao Yong , Zhang Hongfu 
Published: December 2021

Abstract
BackgroundAlthough, the cecum plays vital roles in absorption of water, electrolytes, and other small molecules, and harbors trillions of commensal bacteria to shape large intestine immune functions, it is unknown the cecum development potentials at single cell level during the very crucial neonatal developmental period.ResultsUsing singe cell RNA-seq and proteomics, we have characterized six major types of cecal epithelial cells: undifferentiated cells; immune cells (Ims); cecumocytes (CCs); goblet, Paneth like cells (PLCs), and enteroendocrine cells (EECs) with specific marker genes. CCs mature with a gradual decrease in number; however, Ims develop with a continuing increase in number. Meanwhile secretory cells (goblet, PLCs, EECs) reduce in number during the neonatal developmental period. The cells exhibit specific development and maturation trends controlled by transcriptional factors, ligand-receptor pairs, and other factors. As piglets grow, cecal content and mucosal microbial diversity increases dramatically with population of beneficial microbiota, such as lactobacillus, increasing significantly. Moreover, cecal mucosal-associated and cecal content microbiota are positively correlated and both show significant correlation with different types of cecal epithelial cells and plasma metabolites. ConclusionsThis is the first presentation of neonatal cecal cell development and maturation naturally at single cell level with transcript, protein, microbiota and metabolism perspectives. Furthermore, this study provides an important tool for the determination of novel interventions in cecal drug delivery and metabolism studies.
miR-221-3p Promotes Pancreatic Cancer Progression via Targeting P53 and PTEN
Authors: Wu Jian-Jun , Shang Zhong-Hua , Guo Min , Yang Zhen-Hua , Ren Fang-Gang , Bian Si-Cheng 
Published: December 2021

Abstract
Background: To assess the regulatory role of microRNA-221-3p (miR-221-3p) as a modulator of pancreatic cancer (PC) cell apoptosis, invasion, migration, and proliferation through its ability to regulate P53 and PTEN expression.Methods: PCPATU8988 cells were used as a model in which high-level or low-level models of miR-221-3p expression were established, with qPCR being used to confirm transfection efficiency. CCK-8 assays were employed to evaluate the proliferation of these cells, with migration and invasion being assessed through appropriate in vitro assays. Western blotting was used to assess PTEN and P53 protein levels in these experimental cells. Flow cytometry was additionally used to assess the impact of experimental manipulations on cellular apoptosis.Results: MiR-221-3p overexpression enhanced the migratory, proliferative, and invasive activity of PCPATU8988 cells (P<0.01) and suppressed their apoptotic death (P<0.05), while miR-221-3p inhibition had the opposite impact. No differences in P53 and PTEN protein levels were detected when comparing the NC miR-221-3p mimic or inhibitor groups (P>0.05), whereas miR-221-3p mimic transfection significantly reduced the levels of these proteins (P<0.05).Conclusion: This analysis showed that miR-221-3p can drive PC cell proliferative, migratory, and invasive activity while suppressing the apoptotic death of these cells. Functionally, this miRNA can suppress P53 and PTEN protein expression. Overall, this suggests that miR-221-3p can regulate PC development by controlling the expression of these key oncogenic proteins.
Empirical Models to Formulate the Nonlinear Response of Rocking Shallow Foundations
Authors: Hamidpour Sara , Shakib Hamzeh , Paolucci Roberto , Correia António , Soltani Masoud 
Published: December 2021

Abstract
This paper aims to introduce a simplified moment-rotation backbone model for exploring the nonlinear behavior of shallow foundations subjected to rocking. The model is developed based on parametric numerical investigations of rectangular footings on dense dry sand, taking advantage of a nonlinear macro-element model verified based on a set of experimental results. Empirical expressions are proposed for rocking stiffness degradation due to gravity loads and foundation rotation as a function of the factor of safety against vertical loads and aspect ratio of foundations. Similar to previous researches, the uplift reference rotation was introduced to explore a new closedform expression appropriate for normalizing the foundation response in a non-dimensional form. The proposed approach for stiffness degradation and nonlinear backbone model of rocking foundations aims to be simple, to minimize the dependence on the variable parameters, and to provide physically sound selections for engineering applications.
Cell Surface SARS-CoV-2 Nucleocapsid Protein Modulates Innate and Adaptive Immunity
Authors: Yewdell Jonathan , Lopez-Munoz Alberto , Kosik Ivan , Holly Jaroslav 
Published: December 2021

Abstract
SARS-CoV-2 nucleocapsid protein (N) induces strong antibody and T cell responses. Although considered to be localized in the cytosol, we readily detect N on the surface of live cells. N released by SARS-CoV-2 infected cells or N-expressing transfected cells binds to neighboring cells by electrostatic high-affinity binding to heparan sulfate and heparin, but not other sulfated glycosaminoglycans. N binds with high affinity to 11 human chemokines, including CXCL12β, whose chemotaxis of leukocytes is inhibited by N from SARS-CoV-2, SARS-CoV-1, and MERS CoV. Anti-N Abs bound to the surface of N expressing cells activate Fc receptor-expressing cells. Our findings indicate that cell surface N manipulates innate immunity by sequestering chemokines and can be targeted by Fc expressing innate immune cells. This, in combination with its conserved antigenicity among human CoVs, advances its candidacy for vaccines that induce cross-reactive B and T cell immunity to SARS-CoV-2 variants and other human CoVs, including novel zoonotic strains.
WITHDRAWN: Analyzing the institutional and regulatory framework for operationalizing the blue economy in Bangladesh: Lessons from Australia
Authors: Rahman Md. Mizanur 
Published: December 2021

Abstract

This study's overarching objective was to assess the suitability and applicability of the existing legal and institutional framework for managing the coastal and marine resources in Bangladesh. Besides, the legal and institutional arrangement of Australia was examined to adopt the best practices for Bangladesh. The glimpse of the potential coastal and marine resources of Bangladesh was assessed to have an idea of the untapped resources' dimensions. Together with, the fundamental challenges faced by the artisanal and small-scale fishers in Bangladesh were critically scrutinized. The empirical data collection followed a multi-approach like mini-symposium, consultation workshops, focus group discussions, case studies, visiting, personal, and key informant interviews. The study revealed that the vast provisioning, regulating, and cultural ecosystem services of the Bay of Bengal, including its coast and mangrove, is mostly unexplored. The regulatory framework in Bangladesh is characterized by jurisdictional overlapping followed by the conflict of interests among the public institutes, which originated from the aged and fragmented laws and ambiguous business allocation; consequently, the artisanal and small-scale fishers suffer a lot. On the flip side, despite some limitations, Australia established sectoral governance enacting strong legislative measures. The local government and community’s right in resource management locally has been institutionalized in Australia, which remains fuzzy in Bangladesh. Taking lessons from Australia, Bangladesh can enact new sectoral laws followed by business reallocation for the line ministries. The study will help policymakers identify the bottlenecks rooted in Bangladesh’s existing regulatory and institutional framework.
A Novel Similarity Measure of Link Prediction in Multi-Layer Social Networks Based on Reliable Paths
Authors: Rezaeipanah Amin 
Published: December 2021

Abstract
Online social networks are an integral element of modern societies and significantly influence the formation and consolidation of social relationships. In fact, these networks are multi-layered so that there may be multiple links between a user’ on different social networks. In this paper, the link prediction problem for the same user in a two-layer social network is examined, where we consider Twitter and Foursquare networks. Here, information related to the two-layer communication is used to predict links in the Foursquare network. Link prediction aims to discover spurious links or predict the emergence of future links from the current network structure. There are many algorithms for link prediction in unweighted networks, however only a few have been developed for weighted networks. Based on the extraction of topological features from the network structure and the use of reliable paths between users, we developed a novel similarity measure for link prediction. Reliable paths have been proposed to develop unweight local similarity measures to weighted measures. Using these measures, both the existence of links and their weight can be predicted. Empirical analysis shows that the proposed similarity measure achieves superior performance to existing approaches and can more accurately predict future relationships. In addition, the proposed method has better results compared to single-layer networks. Experiments show that the proposed similarity measure has an advantage precision of 1.8% over the Katz and FriendLink measures.
Evaluation of Rockfall Hazard Based On UAV Technology And 3D Rockfall Simulations
Authors: Utlu Mustafa , ÖZTÜRK Muhammed Zeynel , Şimşek Mesut 
Published: December 2021

Abstract
In this study, the rockfall hazard in Hacıabdullah village located in the Central Anatolia region of Turkey was assessed with three-dimensional (3D) rockfall analyses based on unmanned aerial vehicle (UAV) technology using RAMMS (Rockfall software). With several rockfall disasters experienced in the village, the final event occurred in 2008, and several houses were evacuated due to rockfall risk after this event. A total of 17 hanging blocks with fall potential were identified and block dimension measurements were performed during field studies. In order to assess the rockfall hazard in the study area, digital surface model (DSM) data were obtained using high-resolution images obtained by UAV. According to dimensional values, the geometric and volumetric features of each rock were assessed close to reality with the RAMMS 3D rockfall modeling program. As a result of 3-D rockfall modelling, the maximum kinetic energy, maximum velocity, and maximum jump height of the falling blocks are reached to 3476 kJ, 23.1 m/s, and 14.57 m, respectively. The shape and volume of the blocks, as well as the slope features, rocks display differences in their runout distances after falls. A rock block with equant geometry has a runout distance of 53.1-126.9 m, whereas a rock block with flat or long geometry has a runout distance of 34-122.9 m. Rocks that do not move very far from the source area are; in other words, where the free-fall process is dominant, may significantly damage the roads. However, rolling blocks, in other words, blocks which can travel long distances from the source area, have a potential to cause great damage at the settlement areas, roads and trees. According to the hazard map, R6, R12, R13, R14, R15, R16, and R17 blocks involve high and moderate levels of risk for settlement units. R1, R4, R7, R8, R9, and R10 blocks show that the majority of them involve low risk, while a small portion is a moderate risk.
The Effects of Family-Centered Empowerment Model on Depression, Anxiety, and Stress of the Family Caregivers of Patients with COVID-19: A Randomized Clinical Trial
Authors: Nia Mohammad Namazi , Mohajer Samira , Bagheri Nasser , Sarboozi-hoseinabadi Tahere 
Published: December 2021

Abstract
Background: Taking care of patients with Covid-19 is regarded as a challenging task for family caregivers. A family-centered empowerment model (FCEM) should help them achieve greater psychological strength in the care process.Methods: Seventy caregivers were randomized to FCEM (n= 35) or control (n= 35). The four-stage of FCEM in four online sessions through WhatsApp was provided to the intervention group from the patient's discharge until 2 weeks later. The demographic information questionnaire and Depression Anxiety Stress Scale-21 were employed both before and five days after the FCEM sessions to gather the necessary data.Results: Caregivers included 55.8% women and 44.2% men, with a mean age of 42.5 years. The results demonstrated a substantial difference in the average score of stress (p=0.023), anxiety (p=0.003), and depression (p=0.012).Conclusions: The combination of a face-to-face orientation session and online methods of FCEM is likely to lower stress, anxiety, and depression among caregivers. It can be contributed to the practicability, simplicity, and effectiveness of the intervention.Clinical Trial Registration code: This study (IRCT20180429039463N2) was registered in Iranian Registry of Clinical Trial on 10/04/2020
The Inhibitory Impact of Schisandrin Against LPS-Induced Acute Kidney Injury in Vitro and in Vivo
Authors: Li Weifeng , Huang Qiuxia , Yu Jinjin , Yu Jiabao , Yang Yajie , Liu Yang , Song Huixin , Cui Langjun , Niu Xiaofeng 
Published: December 2021

Abstract
Schisandrin (Sch) is a main bioactive component of Schisandra sphenanthera Rehd.et Wils. It has been reported that Sch could regulate inflammatory disease. This study evaluated the anti-inflammatory and anti-oxidant effects effect of Sch on lipopolysaccharide (LPS)-induced macrophages activation and acute kidney injury mice. Male Kunming mice were intraperitoneally injected with LPS (15 mg/kg) after administration of Sch (12.5, 25, 50 mg/kg) seven days for developing acute kidney injury vivo model. RAW264.7 macrophages were pretreatment Sch (10, 20, 40 µM) and administrated LPS (1 µg/ml) to create an in vitro injury model. ELISA results found that Sch administration reduced the production of inflammatory factors induced by LPS in kidney tissues and RAW264.7 macrophages. It has been observed that Sch alleviated oxidative stress by reducing the levels of reactive oxygen species, myeloperoxidase and malondialdehyde, and increasing the activity of superoxide dismutase and glutathione peroxidase. Hematoxylin-eosin staining data suggested that Sch administration significantly reduced inflammatory cell infiltration and the kidney tissue damage induced by LPS. The blood urea nitrogen and creatinine levels were also reduced by Sch treatment. In addition, Western blot and immunohistochemical analysis showed that Sch up-regulated the expression of Nrf2 and HO-1, and decreased the expression of p-p38, p-JNK, p-ERK1/2, p-IκBα, p-NF-κBp65 and TLR4. The current research showed that Sch reduced LPS-induced acute kidney injury by inhibiting inflammation and oxidative stress, and provided insights into potential ways to treat AKI.
Waterborne and Airborne Microfibers Shed from Non-Woven Materials in Water and Air Environments
Authors: Kwon Soojin , Zambrano Marielis C. , Venditti Richard A. , Frazier Ryen , Zambrano Franklin , Gonzalez Ronalds W. , Pawlak Joel Justin 
Published: December 2021

Abstract
Nonwoven products are widely used in various fields, including many disposable products, such as wipes, diapers, and masks. However, microfibers shed from these products in the aquatic and air environment have not been fully described. In the present study, several commercial single-use nonwoven products and a series of meltblown nonwoven materials produced in a pilot plant were investigated regarding their microfiber generation during their use in aquatic and air environments. Microfibers shed in water were studied using a Launder Ometer equipment (1- 65 mg of microfibers per gram material), and microfibers shed in air were evaluated using a dusting testing machine that shakes a piece of the nonwoven back and forth (~0 to 6000 microfibers (4 mg of microfibers) per gram material). The raw materials and bonding technologies applied to the commercial nonwovens affected the microfiber generation both in water and air conditions. Meltblown nonwoven fabrics generated fewer microfibers compared to the other commercial nonwovens studied here, and the manufacturing factors, such as DCD (Die to collector distance) and air flow rate, affected the tendency of microfiber generation. Microfibers of nonwovens shed in water and air environment were compared to selected textile materials and paper tissue materials. The results herein suggest that it is possible to control the tendency of microfiber shedding through the choice of operating parameters during nonwoven manufacturing processes.
The clinical fabrication of polyolefin mouthguards formed over fixed orthodontic appliances
Authors: Westerman Bill , Kerr Michael , Tod Marcus 
Published: December 2021

Abstract
Using a new polyolefin material without impressions and models, fifty-two mouthguards were fabricated at chairside by a dental professional. Patient acceptance, operator assessment, mouthguard retention and fabrication time were assessed. The study demonstrated the practicality of custom-made mouthguard fabrication over fixed orthodontic appliances and recommends the procedure as a useful adjunct to orthodontic practice.
Çağdaş Siyaset Felsefesine Giriş
Authors: ÖZTÜRK Alper 
Published: December 2021

Will Kymlicka, Çağdaş Siyaset Felsefesine Giriş adlı eserinde siyasi kavramların
tartışıldığı geleneksel kategorilerin (sağ ve sol) darlığı ve yetersizliği nedeniyle bugün
siyaset felsefesi alanında yapılan önemli çalışmaları anlamanın mümkün olmadığını öne
sürer. Sözü geçen eserinde Kymlicka, her siyasi kuramın farklı bir nihai değere sahip
olduğunu göz önünde bulundurarak siyaset felsefesini ahlaki bir zeminde tartışır. Düşünür
bu eserinde, sağ özgürlük ve sol eşitlik gibi zıt değerlerin yanı sıra sözleşmeye dayanan
uzlaşma, ortak yarar, fayda, haklar, kimlik ya da androjini gibi farklı nihai değerlere sahip
siyaset kuramlarını bir araya getirerek adaletin ulaşılmaz bir hedef olmaktan çıkarılmasına
yönelik kapsamlı bir çalışma sunar.
Experimental analysis of cutting force during machining difficult to cut materials under dry, mineral oil, and TiO2 nano-lubricant
Authors: Okokpujie I. P. , Tartibu L. K. 
Published: December 2021

Difficult-to-machine materials, e.g., Titanium alloys, are highly applicable in diverse industries that yield strength and wear resistance. However, they prove difficult to machine due to high vibration, leading to high cutting forces during the machining process. This vibration occurs from chip discontinuity and thereby leads to high friction between the cutting tool and workpiece. In order to minimize these challenges, lubricants are employed in machining operations to reduce frictional and other unnecessary cutting forces and improve surface finish. This research focuses on studying the nano-lubricant effects in reducing cutting forces in the machining of TI-6AL-4V-ELI alloy. Also, carry out a comparative study of dry, mineral oil, and TiO2 nano-lubricant during face-milling machining for optimal performance. Additionally, the study develops a predictive mathematical model for cutting force using a Taguchi L9 orthogonal array. A two-step approach was employed to develop the nano-lubricant before the machining process. The dynamometer is used to collect the cutting force data at the end of each sample. The Results show that the lubrication conditions play a significant role in the reduction of cutting forces. The mineral oil-based-TiO2 nano-lubricant reduces the cutting force by 19 % compared with the mineral oil during the machining of TI-6AL-4V-ELI alloy. Furthermore, the optimal parameters to reduce cutting forces during face milling of TI-6AL-4V-ELI alloy are cutting speed at 3000 rpm, 200 mm/min feed rate, 0.3 mm depth of cut to obtain the minimum cutting force 30 (N). This study concludes that the application of TiO2 nanoparticles in mineral oil significantly improves the thermal and mechanical properties, which leads to a reduction of cutting force.
A cultura material como recurso didático no ensino de História
Authors: Da Silva Micheli Gomes , Da Silva Amanda Kelly Acioli , Pinheiro Helena Alves de Souza de Lucena 
Published: December 2021

Introdução: Este relato de experiência decorre de nossa atuação junto ao Programa Institucional de Bolsas de Iniciação à Docência (PIBID) – Subprojeto História/ Natal. Em 2016, desenvolvemos na Escola Estadual Professor Josino Macedo, um projeto interdisciplinar voltado para os alunos do 6º e 7º Ano do Ensino Fundamental, articulando Arqueologia e História, numa abordagem que buscou privilegiar a História Local e a História Indígena. Objetivos: A proposta era estimular entre os discentes um olhar investigativo, crítico e inquiridor voltado para o espaço local, em geral negligenciado pelos livros didáticos, que tendem a privilegiar os espaços identificados com o poder hegemô- nico. O desafio, nesse caso, era a utilização de recursos didáticos que favorecessem o estudo da História Local, preferencialmente enfocando temas obrigatórios no currículo da área, caso da História e Cultura Indígena. Metodologia: Partindo do pressuposto de que o ensino de História deve ser feito em consonância com método de produção da ciência de referência, para atingir nossos objetivos, optamos por explorar fontes tradicionalmente utilizadas por historiadores em suas pesquisas: vestígios da cultura material de sociedades antigas. Acerca do método de análise, o diálogo com a Arqueologia foi indispensável para que pudéssemos analisar esses vestígios e utilizá-los como recurso didático. Resultados: O resultado da iniciativa foi a realização do I Encontro de Estudos Históricos no espaço escolar, com o tema “O trabalho do arqueó- logo na construção da História”, assim como o planejamento e execução de planos de aula que tinham como principal recurso didático um conjunto de réplicas de materiais líticos e cerâmicos. Discussão: Com essa experiência procuramos contribuir para os debates sobre aprendizagem histórica, colocando em evidência a possibilidade de viabilizarmos a construção do conhecimento numa perspectiva interdisciplinar, por meio do método investigativo, valorizando a História Local. Conclusões: Com essa experiência concluímos que a sala de aula deve ser pensada como um espaço privilegiado para a construção do conhecimento, e que o método de produção do conhecimento histórico pode – e deve – servir de referência para a formulação de propostas inovadoras e criativas de ensino de História.
Universidade Popular do Rio Grande do Norte
Authors: Cascudo Luís da Câmara 
Published: December 2021

O texto de Cascudo com “Universidade popular do Rio Grande do Norte”, original e gentilmente cedido por sua neta Daliana Cascudo, presidente do Ludovicus - Instituto Câmara Cascudo, revela o relatório de uma programação desenvolvida no ano de 1948, do que seria o embrião de uma universidade que se queria popular, com um total de 21 aulas públicas diversificadas como canto gregoriano, doutrinas sociais, psicopatias, e mesmo o combate à malária, ainda a desafiar pesquisadores e órgãos de saúde pública nos dias atuais. Diz ele, finalizando o texto: Aos indiferentes, anedóticos, analgésicos e agressores, dedico um Padre Nosso e uma Ave Maria. A provável incredulidade de alguns certamente deu lugar à construção coletiva de um projeto consolidado em sessenta anos, cujos resultados são comemorados por se associarem intrinsicamente ao desenvolvimento de nosso estado e país.