bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cultural Inputs and Accumulating Inequality in Children’s Reading: A Dynamic Approach
Authors: Blaabæk Ea Hoppe 
Published: November 2021

Abstract
Previous research shows that children from socioeconomically advantaged families read more than children from less advantaged homes. This article studies how inequality in the amount that children read accumulates across childhood and the extent to which this inequality depends on the cultural inputs parents provide. Additionally, the article studies whether children’s or parents’ cognitive ability moderates the effect of cultural inputs. Based on a Dynamic Panel Data Model and data from the National Longitudinal Survey of Youth 1979—Children and Young Adults Supplement, I find that the amount that children read depends on both the cultural inputs they currently receive, but also on those inputs received in previous years (which shaped how much they read in previous years). This cross-time accumulation, coupled with a socioeconomic gradient in the levels of cultural inputs parents provide, leads to growing inequality in children’s reading. I do not find that cultural inputs are more effective in encouraging children with higher ability or children of mothers with higher ability to read more.
Utilizing community InfoSpots for health education: perspectives and experiences in Migoli and Izazi, Tanzania
Authors: Holst Christine , Tschirhart Naomi , Ngowi Bernard , Noll Josef , Winkler Andrea Sylvia 
Published: November 2021

Summary
Limited access to health education can be a barrier for reaching the Sustainable Development Goals, especially in rural communities in sub-Saharan Africa. We addressed this gap by installing community information spots (InfoSpots) with access to the internet and a locally stored digital health education platform (the platform) in Migoli and Izazi, Tanzania. The objective of this case study was to explore the perspectives and experiences of InfoSpot users and non-users in these communities. We conducted 35 semi-structured interviews with participants living, working or studying in Migoli or Izazi in February 2020 and subsequently analysed the data using content analysis.
The 25 InfoSpot users reported variations in use patterns. Users with more education utilized the platform for their own health education and that of others, in addition to internet surfing. High school students also used the platform for practicing English, in addition to health education. Most InfoSpot users found the platform easy to use; however, those with less education received guidance from other users. Non-users reported that they would have used the InfoSpot with the platform if they had been aware of its existence. All participants reported a positive view of the digital health messages, especially animations as a health knowledge transfer tool. In conclusion, different and unintended use of the platform shows that the communities are creative in ways of utilizing the InfoSpots and gaining knowledge. The platform could have been used by more people if it had been promoted better in the communities.
The crystal structure of D-amino acid oxidase with a substrate analog, o-aminobenzoate
Authors: Miyahara Ikuko 
Published: November 2021

Abstract
Since the discovery of D-amino acid oxidase (DAO) in 1935, many studies have been conducted without clarifying its 3D structure for a long time. In 1996, the crystal structure of DAO was determined, and it was shown that the catalytic bases required for the two catalytic mechanisms were not present in the active site. The crystal structure of DAO in complex with o-aminobenzoate was solved and is used for modeling Michaelis complex. The Michaelis complex model provided structural information leading to a new mechanism for reductive half-reaction of DAO. Currently, DAO is being researched for medical and applied purposes.
Lending Relationships and Optimal Monetary Policy
Authors: Bethune Zachary , Rocheteau Guillaume , Wong Tsz-Nga , Zhang Cathy 
Published: November 2021

Abstract
We construct and calibrate a monetary model of corporate finance with endogenous formation of lending relationships. The equilibrium features money demands by firms that depend on their access to credit and a pecking order of financing means. We describe the mechanism through which monetary policy affects the creation of relationships and firms’ incentives to use internal or external finance. We study optimal monetary policy following an unanticipated destruction of relationships under different commitment assumptions. The Ramsey solution uses forward guidance to expedite creation of new relationships by committing to raise the user cost of cash gradually above its long-run value. Absent commitment, the user cost is kept low, delaying recovery.
Individual attributes and inventors matching: A study using data from the Brazilian co-patents network
Authors: Mejdalani Alexandre , Gonçalves Eduardo 
Published: November 2021

AbstractThis paper uses a unique database that combines Brazilian patent data and personal microdata on formal employees from 2000 to 2011 to study the effect of inventor’s individual attributes on the propensity to cooperate. Our main contributions derive from (1) using a dominance coefficient decomposition to examine each individual’s returns based on the non-explicit hierarchy of the link and (2) finding what attributes inventors take into consideration when seeking working partnerships. The main results reveal that inventors tend to collaborate with others with similar educational background and centrality level, and there is a great likelihood of connectivity if educational and centrality levels are high. The dominance coefficient decomposition revealed that a type of social hierarchy emerges in the network.
Relationship Between Subacromial Bone Erosion and Hook Position of Clavicular Plate in Distal Clavicle Fractures
Authors: Shimpuku Eiji , Uchiyama Yoshiyasu , Imai Takeshi , Takatori Naoki , Watanabe Masahiko 
Published: November 2021

Objectives:
To evaluate the influence of posterior hook position on subacromial bone erosion during surgical treatment of distal clavicle fractures with locking compression plate clavicle hook plate (CHP).

Design:
Single-center retrospective study.

Setting:
Level V trauma center (university hospital).

Patients:
Twenty-one patients (mean age, 45.8 years) treated with CHP were included. One, 3, 15, and 2 patients had Neer type I, IIa, IIb, and V fractures, respectively.

Intervention:
Open reduction and internal fixation were performed with locking compression plate CHP. All implants were removed after a mean of 6.1 months postoperatively.

Main Outcome Measurements:
Japanese Orthopaedic Association shoulder scores were used to assess recovery. Radiographically, the coracoclavicular distance was measured as the distance between the tip of the coracoid process and the undersurface of the clavicle. Three-dimensional computed tomography was performed to identify bone erosion at the hook tips after implant removal. The distance from the acromioclavicular joint center to the bone erosion was divided into 5 areas at 5-mm intervals, and the position and depth of bone erosion were measured in each area.

Results:
The mean follow-up period was 17.4 months; mean ± SD Japanese Orthopaedic Association score was 75.9 ± 4.7 at the final follow-up, whereas the mean duration of bony union was 4.4 ± 1.0 (SD) months. Delayed bony union was observed in one patient, whereas a second surgery was required in another due to acromion cut-out. Radiography showed overreduction in 95% of cases. The correlation coefficient showed a difference between coracoclavicular distance and the position of bone erosion measured by CT (Rs = 0.32, P = 0.006). Erosion under the acromion surface was found in all patients. The depth of erosion correlated with the posterior hook position (Rs = 0.29, P = 0.023).

Conclusions:
Posterior hook tip placement increased the likelihood of bone erosion and complications due to overreduction. CHP should be set more anteriorly beneath the acromion to prevent complications.

Level of Evidence:
Therapeutic Level IV. See Instructions for Authors for a complete description of levels of evidence.
Preferences for Shigellosis-Related Health Promotion Materials for Gay, Bisexual, and Other Men Who Have Sex With Men: Results From a Qualitative Assessment, Atlanta, GA
Authors: Respress Ebony , Evener Steve L. , Caruso Elise , Jacobson Kathleen , Bowen Anna , Kachur Rachel E. , Garcia-Williams Amanda G. , Wright Eric R. 
Published: November 2021

Background
Shigellosis, an acute diarrheal disease, is the third most common bacterial infection in the United States. Shigellosis most commonly affects children younger than 5 years; however, clusters and outbreaks of shigellosis have been reported among gay, bisexual, and other men who have sex with men (MSM). Evidence suggests that knowledge of shigellosis among MSM is low, indicating health promotion outreach is needed for this population.

Methods
To inform the development of shigellosis-related health communication materials and strategies, 6 focus groups were conducted in 2017, in Atlanta, GA among 24 self-identified gay and bisexual men. Participants were asked about their preferences and recommendations for health communication materials.

Results
Participants indicated they would prefer a range of physical and virtual materials placed in diverse locations where the community would see them. Respondents recommended health messages be simple, quick to read, and concise with limited word counts. Participants also advised the use of diverse images that were inclusive of couples of varying sexual orientations to reduce stigma. Participants advocated for the use of humor and provocative images to increase user engagement.

Conclusions
The results emphasize the potential benefits of conducting formative research when designing health communication materials. Incorporating messaging preferences of MSM in the development of shigellosis-related health communication materials could enhance their relevance for the target population while also avoiding unintended consequences associated with stigmatizing MSM.
Corpus callosum structural characteristics in very preterm children and adolescents: developmental trajectory and relationship to cognitive functioning
Authors: Siffredi Vanessa , Liverani Maria Chiara , Van De Ville Dimitri , Freitas Lorena G. A. , Tolsa Cristina Borradori , Hüppi Petra Susan , Leuchter Russia Hà-Vinh 
Published: November 2021

AbstractPrevious studies suggest that structural alteration of the corpus callosum, i.e., the largest white matter commissural pathway, occurs after a preterm birth in the neonatal period and lasts across development. The present study aims to unravel corpus callosum structural characteristics across childhood and adolescence in very preterm (VPT) individuals, and their associations with general intellectual, executive and socio-emotional functioning. Neuropsychological assessments, T1-weighted and multi-shell diffusion MRI were collected in 79 VPT and 46 full term controls aged 6 to 14 years. Volumetric, diffusion tensor and neurite orientation dispersion and density imaging (NODDI) measures were extracted on 7 callosal portions using TractSeg. A multivariate data-driven approach (partial least squares correlation) and an age normative modelling approach were used to explore associations between callosal characteristics and neuropsychological outcomes. The VPT and a full-term control groups showed similar trends of white-matter maturation over time, i.e., increase FA and reduced ODI, in all callosal segments, that was associated with increase in general intellectual functioning. However, using age-related normative modelling, findings show atypical pattern of callosal development in the VPT group, with reduced callosal maturation over time that was associated with poorer general intellectual and working memory functioning, as well as with lower gestational age.HighlightsCallosal development was explored in full-term and very preterm (VPT) aged 6 to 15 yearsNeuropsychological, callosal volumetric, tensor and NODDI measures were usedAge-related normative modelling revealed atypical callosal development in VPTIn VPT, atypical callosal maturation was associated with poorer cognitive functioningIn VPT, greater prematurity was associated with increased atypical callosal maturationCRediT rolesVanessa Siffredi: Conceptualization; Data curation; Formal analysis; Investigation; Methodology; Project administration; Software; Visualization; Writing - original draft; Writing - review & editing.Maria Chiara Liverani: Data curation; Investigation; Methodology; Project administration; Writing - review & editing.Dimitri Van De Ville: Methodology; Resources; Software; Supervision; Writing - review & editing.Lorena G. A. Freitas: Data curation; Writing - review & editing.Cristina Borradori Tolsa: Investigation; Project administration; Resources; Supervision; Validation; Writing - review & editing.Petra Susan Hüppi: Conceptualization; Funding acquisition; Methodology; Project administration; Resources; Supervision; Validation; Writing - review & editing.Russia Hà-Vinh Leuchter: Conceptualization; Investigation; Methodology; Project administration; Resources; Supervision; Validation; Writing - review & editing.
An extended amygdala-midbrain circuit controlling cocaine withdrawal-induced anxiety and reinstatement
Authors: Tian Guilian , Hui May , Macchia Desiree , Derdeyn Pieter , Rogers Alexandra , Hubbard Elizabeth , Liu Chengfeng , Bartas Katrina , Carroll Sean , Beier Kevin T. 
Published: November 2021

ABSTRACTWhile midbrain dopamine (DA) neuronal circuits are central to motivated behaviors, much remains unknown about our knowledge of how these circuits are modified over time by experience to facilitate selective aspects of experience-dependent plasticity. Most studies of the DA system in drug addiction focus on the role of the mesolimbic DA pathway from the ventral tegmental area (VTA) to the nucleus accumbens (NAc) in facilitating drug-associated reward. In contrast, less is known about how midbrain DA cells and associated circuits contribute to negative affective states including anxiety that emerge during protracted withdrawal from drug administration. Here, we demonstrate the selective role of a midbrain DA projection to the amygdala (VTADA→Amygdala) for anxiety that develops during protracted withdrawal from cocaine administration but does not participate in cocaine reward or sensitization. Our rabies virus-mediated circuit mapping approach revealed a persistent elevation in spontaneous and task-related activity of GABAergic cells from the bed nucleus of the stria terminals (BNST) and downstream VTADA→Amygdala cells that could be detected even after a single cocaine exposure. Activity in BNSTGABAcells was related to cocaine-induced anxiety but not reward or sensitization, and silencing the projection from these cells to the midbrain was sufficient to prevent the development of anxiety during protracted withdrawal following cocaine administration. We observed that VTADA→Amygdala cells, but not other midbrain DA cells, were strongly activated after a challenge exposure to cocaine, and found that activity in these cells was necessary for the expression of reinstatement of cocaine place preference. Lastly, the importance of activity in VTADA→Amygdala cells extends beyond cocaine, as these cells mediate the development of anxiety states triggered by morphine and a predator odor. Our results provide an exemplar for how to identify key circuit substrates that contribute to behavioral adaptations and reveal a critical role for BNSTGABA→VTADA→Amygdala pathway in anxiety states induced by drugs of abuse or natural experiences as well as cocaine-primed reinstatement of conditioned place preference.
Common proanthocyanidin-rich foods modulate gastrointestinal blooms of Akkermansia muciniphila in a diet-dependent manner
Authors: Chadaideh Katia S. , Eappen Kevin E. , Moore Brandi E. , Carmody Rachel N. 
Published: November 2021

SummaryDeveloping methods to modulate growth of the mucin-degrading gut bacterium Akkermansia muciniphila could benefit patients with different health needs, as A. muciniphila has been associated with both positive metabolic health outcomes and detrimental neurodegenerative outcomes. Growth of A. muciniphila is sensitive to plant-derived polyphenols, and particularly proanthocyanidins (PACs), when administered in isolated form at supraphysiological doses. However, it remains unclear whether doses sufficient for these effects are achievable via diet. Here, we explore the extent to which nutritionally relevant doses of common polyphenol-rich foods – berries, wine, and coffee – influence A. muciniphila abundance in C57BL/6J mice under varying dietary conditions. By administering polyphenol-rich whole foods, comparing polyphenol-depleted and PAC-rich versus PAC-poor food supplements, and through gradient PAC-dosing experiments, we show that PAC-rich foods uniquely induce A. muciniphila growth at doses that are feasibly achieved through routine diet. Notably, the effects of PAC supplementation were detected against a high-fat diet but not a low-fat control diet background, highlighting the importance of habitual diet strategies in either amplifying or mitigating the prebiotic effects of PAC-rich food consumption. Ultimately, our work suggests that both PACs and diet influence A. muciniphila abundance with downstream impacts for human health.
Niche separation in cross-feeding sustains bacterial strain diversity across nutrient environments and may increase chances for survival in nutrient-limited leaf apoplasts
Authors: Murillo-Roos Mariana , Abdullah Hafiz Syed M. , Debbar Mossaab , Ueberschaar Nico , Agler Matthew T. 
Published: November 2021

AbstractThe leaf microbiome plays a crucial role in plant’s health and resilience to stress. Like in other hosts, successful colonization is dependent on multiple factors, among them, resource accessibility. The apoplast is an important site of plant-microbe interactions where nutrients are tightly regulated. While leaf pathogens have evolved elaborate strategies to obtain nutrients there, it is not yet clear how commensals survive without most of these adaptations. Resource limitation can promote metabolic interactions, which in turn shape and stabilize microbiomes but this has not been addressed in detail in leaves. Here, we investigated whether and how the nutrient environment might influence metabolic exchange and assembly of bacterial communities in Flaveria trinervia and F. robusta leaves. We enriched bacteria from both plant species in-vitro in minimal media with sucrose as a carbon source, and with or without amino acids. After enrichment, we studied the genetic and metabolic diversity within the communities. Enriched Pseudomonas koreensis strains could cross-feed from diverse leaf bacteria. Although P. koreensis could not utilize sucrose, cross-feeding diverse metabolites from Pantoea sp ensured their survival in the sucrose-only enrichments. The Pseudomonas strains had high genetic similarity (∼99.8% ANI) but still displayed clear niche partitioning, enabling them to simultaneously cross-feed from Pantoea. Interestingly, cross-feeders were only enriched from F. robusta and not from F. trinervia. Untargeted metabolomics analysis of the leaf apoplasts revealed contrasting nutrient environments, with greater concentrations of high-cost amino acids in F. trinervia. Additionally, P. koreensis strains were better able to survive without a cross-feeding partner in these richer apoplasts. Thus, cross feeding might arise as an adaptation to cope with nutrient limitations in the apoplast. Understanding how apoplast resources influence metabolic interactions could therefore provide plant breeders targets to manipulate leaf microbiome shape and stability.
OaAEP1-mediated PNA-protein conjugation enables erasable imaging of membrane protein
Authors: Lu Zhangwei , Liu Yutong , Deng Yibing , Jia Bin , Ding Xuan , Zheng Peng , Li Zhe 
Published: November 2021

AbstractMethods to efficiently and site-specifically conjugate proteins to nucleic acids could enable exciting application in bioanalytics and biotechnology. Here, we report the use of the strict protein ligase to covalently ligate a protein to a peptide nucleic acid (PNA). The rapid ligation requires only a short N-terminal GL dipeptide in target protein and a C-terminal NGL tripeptide in PNA. We demonstrate the versatility of this approach by conjugating three different types of proteins with a PNA strand. The biostable PNA strand then serves as a generic landing platform for nucleic acid hybridization. Lastly, we show the erasable imaging of EGFR on HEK293 cell membrane through toehold-mediated strand displacement. This work provides a controlled tool for precise conjugation of proteins with nucleic acids through an extremely small peptide linker and facilitates further study of membrane proteins.TOC
ATM-dependent formation of a novel chromatin compartment regulates the Response to DNA Double Strand Breaks and the biogenesis of translocations
Authors: Arnould Coline , Rocher Vincent , Bader Aldo S. , Lesage Emma , Puget Nadine , Clouaire Thomas , Mourad Raphael , Noordermeer Daan , Bushell Martin , Legube Gaëlle 
Published: November 2021

AbstractDNA Double-Strand Breaks (DSBs) repair is essential to safeguard genome integrity but the contribution of chromosome folding into this process remains elusive. Here we unveiled basic principles of chromosome dynamics upon DSBs in mammalian cells, controlled by key kinases from the DNA Damage Response. We report that ATM is responsible for the reinforcement of topologically associating domains (TAD) that experience a DSB. ATM further drives the formation of a new chromatin sub-compartment (“D” compartment) upon clustering of damaged TADs decorated with γH2AX and 53BP1. “D” compartment formation mostly occurs in G1, is independent of cohesin and is enhanced upon DNA-PK pharmacological inhibition. Importantly, a subset of DNA damage responsive genes that are upregulated following DSBs also physically localize in the D sub-compartment and this ensures their optimal activation, providing a function for DSB clustering in activating the DNA Damage Response. However, these DSB-induced changes in genome organization also come at the expense of an increased translocations rate, which we could also detect on cancer genomes. Overall, our work provides a function for DSB-induced compartmentalization in orchestrating the DNA Damage Response and highlights the critical impact of chromosome architecture in genomic instability.
Coupled yawing and orbital rotation in kinesin motility revealed by polarization measurement of gold nanorods
Authors: Sugawa Mitsuhiro , Maruyama Yohei , Yamagishi Masahiko , Cross Robert A. , Yajima Junichiro 
Published: November 2021

AbstractKinesin motor domains generate impulses of force and movement that have both translational and rotational components, raising the question of how the rotational component contributes to motor functions. We used a new assay in which kinesin-coated gold nanorods (kinesin-GNRs) move on suspended microtubules, for two plus-end-directed and weakly-processive kinesins: single-headed KIF1A, dimeric ZEN-4. Polarization of the light scattered by two types of kinesin-GNRs periodically oscillated as they orbited the microtubule along a left-handed helical trajectory. Our analyses revealed that each kinesin-GNR unidirectionally rotates about its yaw axis as it translocates, and that the period of this yaw-axis rotation corresponds to two periods of its left-handed helical orbit around the microtubule axis. Theoretical analyses suggest that the yaw-axis rotation enhances biased lateral displacement of the kinesin team. Our study reveals biaxial rotation as a new mode of motility in kinesin teams that may help the team to sidestep roadblocks.