bluuebunny/crossref_metadata_2025_split huggingface dataset:
MS-Based Metabolomics Reveals Metabolic Dysregulation in Erastin-Induced Gastric Adenocarcinoma Cells
Authors: Liu Ying , Hu Wenchao , Asmamaw Mogesdessale , Pan Lulu , Li Juan , Liu Hongmin 
Published: September 2021

Abstract
Gastric cancer (GC) is one of the most malignant tumors with high morbidity and mortality in the world, particularly in China. Erastin, a classical ferroptosis inducer, exerts cytotoxicity in several types of cancer cells including gastric cancer cells. However, the mechanism of erastin in regulating metabolic pathways in gastric cancer remains largely unclear. To investigate the gastric cellular response to erastin therapy, we adopted a cell pseudotargeted metabolomics method based on liquid chromatography-hybrid triple quadrupole linear ion trap mass spectrometry (LC-QTRAP MS). The developed method was used to investigate the differential metabolites between erastin-treated MGC-803 cells and the controls at different time points. We found that erastin induced tremendous impact on the metabolome of gastric cells by affecting key metabolic processes, such as cysteine and methionine metabolism, glutathione (GSH) biosynthesis, glycolysis and TCA cycle. Interestingly, S-adenosylmethionine (SAM), methionine, serine, glycine and cysteine were obviously increasing treads after erastin treatment, but S-adenosylhomocysteine (SAH) and GSH were always down-regulated up to 48 h. The results indicated that DNA methylation was activated and glutathione biosynthesis was blocked in erastin-treated MGC-803 gastric cells, highlighting the importance of erastin as a promising drug candidate for in vivo treatment of gastric tumor.
Acupuncture and Moxibustion in the Treatment of Chronic Urticaria: A Case Control Study
Authors: Zhang Ye , Zhang Shizhao , Ping Xiangyu , Zhang Zehua , Tang Liheng , He Liyun , Zhang Xin 
Published: September 2021

Abstract
Background: Chronic urticaria is a clinically refractory skin disease with long symptom duration and high recurrence rate. The current research demonstrates that the first-line drugs treated by medicine are the second-generation non-sedation antihistamines. Although the effect is good In the treatment process, the first-line drugs often relapse within a week after withdrawal, which affects the quality of life of patients. Existing studies have shown that acupuncture is safe and effective in the treatment of chronic urticaria. This study is a randomized controlled clinical study designed to provide high-quality evidence for the international use of acupuncture in the treatment of chronic urticaria.Methods: This study is a randomized, double-blind, sham-controlled clinical trial. A total of 60 participants were selected and divided into an acupuncture group and a micro-acupuncture group, with equal numbers in the two groups. The acupuncture group received fixed-point acupuncture treatment, and the micro-acupuncture group received micro-acupuncture treatment (micro-acupuncture at non-acupoints). Patients will receive 4 weeks of treatment, three times per week for a total of 12 treatments. The primary measurement indicator is Urticaria Activity Score (UAS), and the secondary measurement indicators include Visual Analogue Scale (VAS), Dermatology Life Quality Index (DLQI), Hamilton Depression Scale (HAMD), Hamilton Anxiety Scale (HAMA), Pittsburgh Sleep Quality Index (PSQI), adverse events. UAS and VAS were assessed before treatment, during treatment, and 4 weeks, 8 weeks, and 12 weeks after treatment. DLQI was assessed before treatment, in the 4th week of treatment and 4 weeks, 8 weeks, and 12 weeks after treatment. Then HAMD, HAMA and PSQI were measured before treatment and in the 4th week of treatment. Adverse events will be summarized at 1st, 2nd, 3rd, and 4th weeks after randomization.Discussion:This study is a pilot study, and its main purpose is to explore the feasibility and safety of acupuncture in the treatment of chronic urticaria. The research results will help confirm whether acupuncture can improve the quality of life of patients with chronic urticaria, provide evidence that acupuncture can effectively treat chronic urticaria, and provide basic data for further larger-scale experiments.
Metaraminol and Noradrenaline in Septic Shock: A Retrospective Comparison
Authors: Krishnamohan Anirudh , Delaney Anthony , Gillett Mark 
Published: September 2021

Abstract
IntroductionVasopressor use is an important facet of septic shock management, in order to maintain hemodynamic targets and end organ perfusion. Traditionally, Noradrenaline has been the ‘gold standard’ drug of choice for septic shock. Metaraminol is an alternative vasopressor that has been used for septic shock. However, there has been minimal research in comparing the two drugs in septic patients, particularly with regards to total time spent on infusion. ObjectivesTo compare total time spent on either Metaraminol or Noradrenaline infusion by septic shock patients, whilst adjusting for baseline severity of illness. Secondary outcomes included incidence of mechanical ventilation and new requirement of renal replacement therapy, and mortality. MethodsA retrospective medical records review was undertaken, looking at all septic shock patients admitted to ICU in 2019, who received either Metaraminol or Noradrenaline. Data extracted from eRIC (the ICU database) included total time spent on infusion, APACHE III scores, incidence of mechanical ventilation, incidence of renal replacement therapy, and mortality. ResultsOur review yielded 174 patients who were eligible for further statistical analysis (63 in Metaraminol group, and 111 in the Noradrenaline group). The mean duration of infusion in the Metaraminol group was 1655 minutes, and 2663 minutes in the Noradrenaline group. The mean APACHE III Scores were 62 in the Metaraminol group and 77 in the Noradrenaline group. A one-way ANCOVA test found that there was a statistically significant [F(1, 171)=4.511, p=0.035] reduction in time spent on Metaraminol infusion, compared with Noradrenaline, after adjusting for baseline severity of illness by way of APACHE III Score. ConclusionOur study found a statistically significant reduction in time spent on a Metaraminol infusion compared with Noradrenaline by septic shock patients, after controlling for severity of illness. However, due to its retrospective study design, we were unable to account for bias and confounders, such as antibiotic and fluid administration, or clinician preference for one drug over the other. Nevertheless, our study adds to the paucity of literature comparing Metaraminol to Noradrenaline, and paves the way for future randomized trials comparing the two drugs in septic shock.
Biomimetic “Nacre-like” Films Prepared via Layer-by- Layer Self-assembly of Mica, Polyvinyl Alcohol and Polymethyl Methacrylate
Authors: Gao Yining , Liu Peng , Hu Chuanlin , Yang Lu 
Published: September 2021

Abstract
"Mortar" and "brick" structure is a general model to construct nanocomposites. A film with "brick-and-mortar" structure was prepared by LBL (Layer-by-Layer) technique using polyvinyl alcohol (PVA) and polymethyl methacrylate (PMMA) as the flexible material or "mortar" and mica as the rigid material or "brick". The film deposited on a glass slide after self-assembly cycles was ~3μm thick with an uneven, wavy surface. The film showed enhanced mechanical properties with the hardness and indentation modulus values up to 6.14 GPa and 68.41 GPa, respectively. It was found that the hardness and elastic toughness depended on the ratio of mica, the number of self-assembly cycles, and the pretreatment method of the mica suspension. The self-assembly process was believed to be attributable to the hydrogen bonds between the silanol groups of mica and the hydroxyl groups of PVA and carbonyl groups of PMMA.
Biomolecular Characterization of Bacillus tequilensis A1C1 Isolated from Soil and Chromatographic Analysis of D-serine in its Cellular Fraction
Authors: Kapil Shikha , Bhattu Monika , Kumar Tarun , Sharma Vipasha 
Published: September 2021

Abstract
The bacteria isolated from the pomace dumping soil site (bacteria id A1C1) showed maximum growth (O.D600 = 1.97±0.4 X 109 cells/ml) within 48h in the minimal salt media supplemented with L-serine. The isolated strain was identified as ‘Bacillus tequilensis’ through 16sRNA sequencing. The strain was quantified for D-serine content by using RP-HPLC. The D-serine concentration was calculated as 0.919±0.02 nM in the bacterial cellular fraction by using a standard curve plot and linear curve equation. Further, recovery % was also calculated for the spiked samples which vary from 85-90%. The study’s peculiarity reflects the fact that the isolated strain was explored for the first time to detect the presence of serine enantiomers. The biochemical features also showed 70% similarity to the standard strain Bacillus tequilensis 10bT. The optimum growth parameters were recorded as 37℃±0.5, 150±0.5 RPM, and 7±0.5pH. The strain was Gram-positive and synthesized endospores. Morphological results showed its rod shape, large, irregular, and off-white-coloured colonies. A1C1 was also tested for the production of secondary metabolites and enzymes. A1C1 showed positive results for indole production, lactose fermentation, protease, and gelatinase whereas, negative results for catalase, MR-VP, citrate utilization, cellulase, amylase, and pectinase. Further, the strain was assayed for PGPR attributes and showed a negative phosphate solubilization index and IAA production. The antibacterial assay showed 5% and 2% efficacy of the extracellular fraction against MTCC 40 and MTCC 11949 respectively. These results demonstrate that Bacillus tequilensis A1C1 has antibacterial activity, the potential to secrete extracellular enzymes, and D-serine content in the intracellular fraction of the cultivated cells.
Impact of a Simulation-Based Training on Self Assessed Nursing Performance in Anaphylactic Shock Management: A Pre-Post Educational Intervention Study
Authors: Ren Qi , Chen Fang , Zhang Huijuan , Tu Juanhua , Xu Xiaowei , Liu Caixia 
Published: September 2021

Abstract
Background: The New Nurses who lack the ability to recognize and manage anaphylactic shock can endanger the patients. In this study, we explored the effect of a simulated scenario designed to improve nurses’ understanding of their roles and responsibilities during the rescue of a patient with anaphylactic shock.Methods: The program of a simulation-based training was designed to teach learners to recognize the signs and symptoms of anaphylactic shock, place the patient in the correct position, stop ongoing intravenous infusion of the antibiotic which trigger the anaphylactic shock, restart an intravenous infusion on a new infusion apparatus, give 100% oxygen via a nasal cannula or mask, preserve airway patency, call the rapid response team (RRT), and correctly administer the medications prescribed by the clinician. Instructors evaluated each learner’s skills and behaviors by using a clinical competency questionnaire. All learners then completed the Chinese version of the Simulation Design Scale (SDS) and participated in semi-structured interviews with their instructors after the training.Results: All learners showed significant improvements in the 6 competencies assessed by the clinical competency questionnaire after the simulation-based training (all P<0.001). Scores on the SDS revealed that the learners were highly satisfied with all aspects of the simulation-based training (the 20 satisfaction rates were all above 90.00%). During the semi-structured interviews, new graduated nurses reported that simulation-based training in the management of anaphylactic shock was extremely important and would guide them in clinical practice.Conclusions: The simulation-based training in anaphylactic shock is a potentially viable and effective method to teach new registered nurses to manage clinical incidents.
Coping with catastrophe: contributing to food security through crop diversity and crop production in Tigray National Regional State in northern Ethiopia
Authors: Wilson R Trevor 
Published: September 2021

Abstract
Tigray, the most northerly of Ethiopia’s ethnic based Regional States, covers an area of 53 386 km sq and has a population exceeding 5.17 million of whom some 24.3% are urban dwellers. Wide agroclimatic variations result from altitudinal differences (600–3000 + meters) and rainfall patterns. The area is a center of origin of many cultivated and wild plants. The mainly agricultural rural population, farming small areas of non-contiguous plots, makes wide use of these conditions to ensure its livelihood. Crops grown include ten species of cereals, seven pulses legumes), six oilseeds, and numerous fruits, vegetables and spices. Within these groups and species several varieties or landraces are recognized and used to advantage. Wild species are exploited for food, especially at times of crop failure, and for medicines. Crop production is beset by many biotic (weeds, pests, diseases), abiotic (infertile soils) and anthropic (government policy, civil strife, military actions) constraints. Drought is a frequent occurrence and leads to crop failure and famine. In normal times crop yield are low but some of the production is marketed to provide cash for other necessities. Tigray’s population is poverty stricken and often in need of food aid.
Application of Artificial Neural Networks for Corrosion Behavior of Ni-Zn Electrophosphate Coating on Galvanized Steel and Gene Expression Programming Models
Authors: Zeraati Malihe , Chauhan Narendra Pal Singh , Sargazi Ghasem 
Published: September 2021

Abstract
Zn–Ni electrophosphate coating is one of the most commonly used materials in industrial applications. Corrosion resistance of this coating is very important in order to achieve the minimum corrosion current of the Zn–Ni electrophosphate coating. This paper described a new reliability simulation framework to determine the corrosion behavior of coating using gene artificial neural network (ANN) to estimate the corrosion current of the coating. The input parameters of the model are temperature, pH of electroplating bath, current density and Ni2+ concentration and corrosion current defines as output. The effectiveness and accuracy of the model was checked by utilizing absolute fraction of variance (R2=0.9999), mean absolute percentage error (MAPE=0.0171) and root mean square error (RMSE= 0.0002). The determined using genetic algorithm (GA) and the optimum practice condition are proposed.
Development of Simple Measurement Device to Measure Spasticity Based on an Analysis of Clinical Maneuver and Its Concurrent Validity With Modified Ashworth Scale
Authors: Tanikawa Hiroki , Mukaino Masahiko , Itoh Shota , Kondoh Hikaru , Fujimura Kenta , Teranishi Toshio , Ohtuska Kei , Hirano Satoshi , Kagaya Hitoshi , Saitoh Eiichi , Otaka Yohei 
Published: September 2021

Abstract
BackgroundDespite recent developments in the methodology for measuring spasticity, the discriminative capacity of clinically diagnosed spasticity has not been well established. This study aimed to develop a simple device for measuring velocity-dependent spasticity with improved discriminative capacity based on an analysis of clinical maneuver and to examine its reliability and validity.MethodsThis study consisted of three experiments. First, to determine the appropriate motion of a mechanical device for the measurement of velocity-dependent spasticity, the movement pattern and the angular velocity that the clinicians use in evaluating velocity-dependent spasticity were investigated. Analysis of the procedures performed by six physical therapists in evaluating spasticity were conducted using an electrogoniometer. Second, a device for measuring the resistance force against ankle dorsiflexion was developed based on the investigation in the first experiment. Additionally, preliminary testing of validity, as compared to that of the Modified Ashworth Scale (MAS), was conducted on 17 healthy participants and 10 patients who had stroke with spasticity. Third, the reliability of measurement and the concurrent validity of mechanical measurement in the best ankle velocity setting were further tested in a larger sample comprising 24 healthy participants and 32 patients with stroke.ResultsThe average angular velocity used by physical therapists to assess spasticity was 268±77°/s. A device that enabled the measurement of resistance force at velocities of 300°/s, 150°/s, 100°/s, and 5°/s was developed. Based on the analysis of clinical procedures, a stretching motion prior to measurement was added. In the measurement, an angular velocity of 300°/s was found to best distinguish patients with spasticity (MAS of 1+ and 2) from healthy individuals. A measurement of 300°/s in the larger sample differentiated the control group from the MAS 1, 1+, and 2 subgroups (p<0.01), as well as the MAS 1 and 2 subgroups (p<0.05). No fixed or proportional bias was observed in repeated measurements.ConclusionsA simple mechanical measurement device was developed based on the analysis of clinical maneuver for measuring spasticity and was shown to be valid in differentiating the existence and extent of spasticity.Trial registrationUMIN000026305, date of registration: 25 February 2017; jRCTs042180044, date of registration: 21 November 2018; UMIN000040472, date of registration: 21 May 2020.
The impact of complete blood count-derived inflammatory markers on survival in patients with localized renal cell cancer treated with partial or radical nephrectomy – a single center retrospective study
Authors: Zapała Łukasz , Ślusarczyk Aleksander , Garbas Karolina , Mielczarek Łukasz , Ślusarczyk Cezary , Zapała Piotr , Wróbel Andrzej , Radziszewski Piotr 
Published: September 2021

Abstract
BackroundSeveral hematological markers of systemic inflammation were reported as prognostic in renal cell carcinoma (RCC). We aimed to re-evaluate the prognostic significance of clinicopathologic features and compare the predictive value of different inflammatory markers in RCC.MethodsFour hundred ninety-five patients treated with nephrectomy for primary localized or locally advanced RCC were included in the retrospective analysis. The median follow-up was 48 months.ResultsPatients with higher neutrophil/lymphocyte ratio (NLR), platelet/lymphocyte ratio (PLR), systemic inflammatory response index (SIRI), systemic immune-inflammation index (SII), neutrophil/erythrocyte ratio (NER), derived neutrophil/lymphocyte ratio (dNLR), and lower lymphocyte/monocyte ratio (LMR) and hemoglobin/platelet ratio (HPR) had worse cancer-specific survival (CSS). In the multivariate analysis tumour stage, grade, age and high SIRI constituted independent factors predicting CSS. The model including SIRI values achieved C-index 0.903. Alternative multivariate models that included SII and NLR were characterized by comparable C-indexes i.e. 0.902 and 0.890, respectively. Different inflammatory markers could not be utilized in a single model, as they show a strong correlation with one other (SII, SIRI and NLR), and seem to provide similar prognostic information. Tumour grade and diameter were independent predictors for recurrence-free survival, whereas age, tumour grade and high NER (or high SIRI/ SII in alternative models) were prognostic for overall survival.ConclusionsMarkers of systemic inflammation might provide additional prognostic information (especially SIRI, SII, NLR and NER) and further increase the predictive accuracy of already available models in localized and locally advanced renal cell carcinoma. Clinicopathological features (stage, grade and age) remain the most important prognostic factors for oncological outcomes in RCC patients treated with nephrectomy. For the first time, we show the prognostic value of neutrophil-to-erythrocyte ratio, which constitutes an independent risk factor of overall survival.Trial registrationnot applicable
Kechuangping Mixture Inhibits LPS‑induced Macrophage Immune Response Through Suppressing the TLR4/MyD88/NF-κB Signal Pathway
Authors: Lyu Shuang , Liangtao Luo , Yang Aijun , Yang Lijun , Cui Hong 
Published: September 2021

Abstract
Background: Kechuangping mixture (KCPM) based on Maxingshigan decoction has a significant clinical effect in the treatment of pediatric pneumonia, especially for asthmatic pneumonia. This study explored the effects of KCPM on lipopolysaccharide (LPS)-induced macrophage immune response and the molecular mechanism involved. Methods: CCK-8 assays was used to detect the effect of KCPM on cell viability. The RAW 264.7 macrophages were divided into four groups: blank, LPS, KCPM, LPS+KCPM. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was used to examine the secretion of inflammatory factors and the expression of TLR4/MyD88/NF-κB signal pathways with or without KCPM stimulation. Results: KCPM inhibited the differentiation of RAW264.7 macrophages induced by LPS. The secretion of pro-inflammatory cytokines IL-6 and TNF-α induced by LPS was decreased by pre-treatment with KCPM. While, treatment with KCPM advanced, the anti-inflammatory cytokines IL-10 and TGF-β increased significantly. Additionally, KCPM exhibited a potent inhibitory effect on the expression of iNOS and a promotive effect on the expression of Arg-1. Moreover, KCPM clearly suppressed the transcription level of TLR4, MyD88 and NF-κB signal pathways.Conclusion: KCPM promoted the macrophages polarization toward to M2 phenotype, as a result, limited the macrophage immune response. TLR4/MyD88/NF-κB signal pathways may play an important role in this process.
Preparation of Borneol Angelica Polysaccharide Liposomes and Study Evaluation of Its Anti-Cerebral Ischemia-Reperfusion Inflammatory Reaction
Authors: Wang Ke , He Lin Qin , Yang Xiao Yu , Huang Hai Hong , Liu Wen Long , Xiao Chen , Du Ze Xuan , ding huang , Zhang Wei 
Published: September 2021

Abstract
Objective To prepare borneol angelica polysaccharide liposomes (BAPLs) with distinct characteristics in Chinese and Western medicine, to explore their effects on anti-cerebral ischemia-reperfusion inflammatory reactions and to explain their mechanism. Methods Under the guidance of the basic theory of traditional Chinese medicine, a new dosage form of nanoliposomes was selected to prepare BAPL by the thin film dispersion ultrasonic method. The effects of angelica polysaccharide (AP), lecithin, cholesterol and ultrasonication time on encapsulation efficiency were investigated. Triphenyltetrazolium chloride (TTC) staining was used to detect the volume of cerebral infarction. Western blotting was used to detect the expression levels of TLR-4, NF-κBp65, ZO-1, ZO-2, IL-1β, IL-6, IL-8 and IL-10. Results As the mass ratio of lecithin to cholesterol (X1), the mass of angelica polysaccharide (X2) and the interaction between X1 and X2 (X1X2) were used as investigation factors, the fitting equation between encapsulation efficiency Y and X was Y=0.307-0.153X1+0.026 X2+0.006X12-0.000301 X22+0.002X1X2 (P＜0.05). The best ultrasonication time was 15 min, the highest encapsulation efficiency was 80.4%, the particle size was 179.1 nm, and the surface zeta potential was -17.2 mV. In BAPL group, the infarct volume of TTC staining was significantly decreased (P＜0.05), and the expression levels of NF-κBp65, TLR-4, IL-8, IL-6, IL-1β in brain tissue were significantly decreased (P＜0.05), while the expression levels of ZO-1, ZO-2, IL-10 were significantly increased (P＜0.05). Conclusion BAPL has high encapsulation efficiency, small particle size and stable properties and can alleviate cerebral ischemia-reperfusion injury. Its mechanism may be related to inhibiting inflammatory reactions, downregulating inflammatory reaction signaling pathways and protecting the blood–brain barrier (BBB).