bluuebunny/crossref_metadata_2025_split huggingface dataset:
Melatonin alleviates hydrogen peroxide induced oxidative damage in MC3T3-E1 cells via SIRT1/p66SHC pathway and promotes osteogenesis
Authors: Liu Hedong , Ren Maoxian , Li Yang , Zhang Ruotian , Ma Nengfeng , Li Tianlin , Jiang Wenkai , Zhou Zhi , Yao Xuewei , Liu Zhiyi , Yang Min 
Published: October 2021

Abstract
Oxidative stress is an important contributor to the development of osteoporosis. Melatonin, an indoleamine secreted by the pineal gland, has antioxidant properties. This study aims to explore whether melatonin can promote bone formation and elucidate the mechanisms underlying this process. In this study, we used an in vitro hydrogen peroxide (H2O2)-induced oxidative stress model in MC3T3-E1 cells and an in vivo ovariectomized osteoporotic bone defect model in rats to explore the protective effects of melatonin against osteoporotic bone defects along with the mechanism underlying these effects. We found that melatonin significantly increased alkaline phosphatase activity, mineralization capacity, and the expression of BMP2, RUNX2, and OPN in MC3T3-E1 cells. Furthermore, melatonin was found to activate SIRT1 and inhibit p66SHC, reduce the intracellular reactive oxygen species levels, stabilize mitochondria, reduce malondialdehyde levels, increase superoxide dismutase activity, and reduce apoptosis in MC3T3-E1 cells treated with H2O2. Intriguingly, these effects could be reversed by the SIRT1 inhibitor EX527. In vivo experiments confirmed that melatonin improves the microstructure and bone mineral density of the distal femoral bone trabecula and promotes bone formation. Taken together, our findings showed that melatonin can restrain oxidative damage in MC3T3-E1 cells via SIRT1/p66SHC pathway and promote osteogenesis, suggesting that melatonin could be a potential therapeutic agent for osteoporosis-related bone metabolic diseases.
Impact of an Automated Internet-Based Cognitive Behavioral Therapy Program on Suicide Thinking and Risk Among US Rural Adults
Authors: Schure Mark B. , McCrory Bernadette , Greist John , Weissman Ruth Striegel 
Published: October 2021

Abstract
Cognitive Behavior Therapy (CBT) is an evidence-based psychotherapy for mental disorders, including depression. Internet-based CBT (iCBT) programs increasingly are showing similar impact to clinician-delivered CBT. We assessed the impact of Thrive, a fully-automated iCBT depression treatment program on suicidal thinking. Participants were randomly assigned to Thrive (n=218) or a waitlist control group (WLC, n= 230). Intent-to-treat analyses tested for group differences at 8-weeks in suicidal thinking (CHRT-SR3 subscale, primary outcome), and secondary outcomes including depression symptoms (PHQ-9), anxiety symptoms (GADS-7), work and social adjustment (WSAS), and resilience (CD-RISC-10). Using self-reports, participants were evaluated at baseline, 4 and 8 weeks for each outcome. Thrive program adherence (n = 218) was assessed by number of lessons completed. The Thrive group was 38.7% less likely than the control group to present with suicidal thinking at 8 weeks (odds ratio 0.61, p = 0.10). Comparison of 8-week depression symptom slopes showed statistically significant effects favoring the Thrive group (WLC = -3.04 vs. Thrive = -4.32, p = 0.007) (d = 0.08); no other significant group differences were observed. Lessons completed were significantly related to lower PHQ-9 (p = 0.026) and GAD-7 scores (p < 0.01). Study results are consistent with a previous study showing nonsignificant effect of an automated iCBT program for reducing suicidal thinking, but a significant positive impact on depression symptoms among rural US adults. Future studies should test whether strategies for boosting lesson completion are successful in enhancing the efficacy of Thrive to reduce suicide risk.
CXCL10 Expression in Tumor Tissues and is An Independent Prognostic Indicator of Pancreatic Cancer
Authors: Hu Lingyu , Xu Haokai , Zhong Zhengxiang , Wu Bin 
Published: October 2021

Abstract
Background: Pancreatic cancer is a malignant disease with difficult diagnosis and high mortality. The most common type is pancreatic duct adenocarcinoma (PDAC). CXCL10 is of great significance in the treatment of immune diseases and cancer. Here, we evaluated the expression of CXCL10 in pancreatic cancer and its clinical significance. Results: The results of the TCGA database showed that the expression of CXCL10 in pancreatic cancer tissues was higher than that in matched adjacent tissues (P <0.05). Patients with high expression of CXCL10 had a poor prognosis compared with those with low expression of CXCL10 (P=0.0051).We found that CXCL10 was positively and strongly correlated with tumour-infiltrating neutrophils and macrophages in PDAC (P<0.0001). We also performed CXCL10 immunohistochemical staining in a tissue microarray (TMA), which included tumour samples collected from 119 PDAC patients (each sample was matched with an adjacent tissue sample). The expression level of CXCL10 in PDAC tissues was prominently higher than that in the matched adjacent tissues (P = 0.0001). Analysis of clinicopathological factors suggested that the high expression of CXCL10 was correlated with the patient's T stage (P = 0.020). In addition, higher CXCL10 expression was significantly associated with worse overall survival of PDAC patients (P = 0.012). The results of COS multivariate analysis suggested that age (P=0.005), histological grade (P=0.001), TNM stage (P=0.017) and CXCL10 expression (P=0.024) were independent prognostic factors for pancreatic cancer. Conclusion: Our data indicate that CXCL10 is highly expressed in PDAC and may be used as an independent clinical prognostic indicator for PDAC.
A Biochemiluminescent Assay for Rapid Diagnosis of Influenza
Authors: Lin Xuexiang , Gu Jia 
Published: October 2021

Abstract
Background: A biochemiluminescent assay of influenza diagnosis is presented. The assay diagnoses influenza based on detection of the influenza viral neuraminidase activity. An instrument designed for the assay is also reported. This assay solves the problem that current influenza virus diagnosis assays are susceptible to virus mutation. Methods: A luciferase-based complex is synthesized as biochemiluminescent substrate. The substrate is cleaved to free luciferin with presence of influenza neuraminidase in specimen. Luciferase is oxidized to oxyluciferin with luciferin as catalyzer resulting in luminescence, which is proportional to the neuraminidase activity and measured by instrument. The instrument uses a photomultiplier tube (PMT) as sensor, with 24 test channels. Fine optical arrangements enable the instrument with high sensitive and accuracy. Results: A total of 389 clinical specimens were collected to evaluate the performance of the assay in clinical settings. This assay had a sensitivity and specificity of 95.92% (95% confidence interval: 91.38%-98.12%) and 97.93% (95% confidence interval: 95.26%-99.11%), respectively, compared to the colloidal gold assay. Conclusion: As a biochemiluminscence assay, this assay is advantageous in sensitivity and specificify. It does not require any washing and separation steps, which makes the instrument simple in design and easy to operate or maintenance. The assay is suitable for the rapid diagnosis of influenza virus in POC settings.
Evaluation of Genetic Diversity by Morphological, Biochemical and Molecular Markers in Sour Cherry Genotypes Collected from the Kelkit Basin of Turkey
Authors: YAMAN MEHMET 
Published: October 2021

Abstract
BackroundTurkey's ecological and geographical diversity allows the cultivation of most plant species that have economic importance throughout the country. One of these species, sour cherry, is among the stone fruits that are very important for human health. In this study, it was aimed to determine by morphological, biochemical, and molecular marker technique (ISSR) in the genetic diversity of 21 sour cherry genotypes grown from seed in Kelkit Valley and standard Kutahya cultivars.Methods and ResultsIn the results of the study, genotype 22 (especially fruit weight) in terms of fruit characteristics and genotype 20 in terms of leaf characteristics produced better results than the other genotypes. In molecular marker analysis, a total of 70 bands were obtained from 12 ISSR primers and 49 of them were polymorphic. The mean polymorphism rate in the study was 70%, and the DICE's similarity coefficient of genotypes differed between 0.81 and 0.95. Polymorphism percentages of the ISSR primers varied from 20–100%. In biochemical analyzes, G17 with 140.18 mg QE/100 g in total flavonoid content, G19 with 208.63 mg GAE/100 g in total phenolic content, G19 with 8.33 mg cyn-3-gluc /100 g in total anthocyanin content compared to other genotypes prominent genotypes.ConclusionThe use of the obtained results in breeding studies to be carried out on this species, as well as the detection and protection of gene sources, may be beneficial for researchers.
Curved Interference Pattern Created by Photons Behaving as Particle---Double Slit Experiment Still Has Much to Offer
Authors: Peng Hui 
Published: October 2021

Abstract

Young’s double slit experiments, which represent the mystery of quantum mechanics, have been described by either the classical wave, or quantum probability waves or pilot waves. Recently, the novel experiments show that the interference patterns of the double slit/cross-double slit experiments may be curved. The previous phenomena of the light bending contain the gravity bending and Airy beam curving transversely. The curved Airy beam is interpreted by the quantum Schrödinger’s wave equation and electromagnetic wave theory. To study the curved interference patterns of the comprehensive double slit experiments, we study the underlying physics first, namely, to study whether the light beam/photons behave as wave or as particle before forming the curved interference pattern. In this article, the comprehensive double slit experiments are performed, which show: (1) the fringes of the curved interference pattern are created independently and may be create partially; (2) the longitudinal shield and the metal tube inserted between the slide and the detector has no effect on the interference pattern. The experimental observations suggest that, before forming the curved interference pattern on the detector, photons behave as particles, which can be referred as “wave-particle-coexistence”. The phenomena provide the comprehensive information/data for the theoretical study.
An extended family of perforated submicrometer hollow or core-shell plasmonic particles as antireflective synthetic brochosomes
Authors: Jakšić Zoran , Obradov Marko , Jakšić Olga , Tanasković Dragan 
Published: October 2021

Abstract
We investigated different biomimetic structures inspired by natural brochosome powders which appear on the bodies and wings of leafhoppers (insects from the Cicadellidae family). All structures we analyzed are roughly spherical, with diameters 200 nm to 1000 nm, with a core and a shell made of different materials and the core perforated with subwavelength holes with diameters of the order of tens of nanometers. We extended the range of possible designs, geometries and materials of synthetic brochosomes, inspired by their natural counterparts found as powders secreted by various species of leafhoppers. We performed simulation of the optical properties of the structures using the finite element method. We found out that our approach ensures the design of highly efficient omnidirectional ultra-antireflective diffractive powders with subwavelength apertures. The reflectivity of 600 nm diameter holey spheres does not exceed 0.02% in 500-600 nm range. We showed that planar arrays of plasmonic-based artificial brochosomes exhibit a rich optical behavior, including effective refractive index below unity and even below zero at longer wavelengths. Such metamaterial-like behavior contributes to the multifunctionality of our synthetic brochosomes which can already serve as antireflective, superhydrophobic and highly porous structures controllable by design. This kind of versatility shows potentials for numerous practical uses. A major part of the novel functionalities stems from the use of nanocomposites containing free-electron conductors (plasmonic materials). Thus we arrived at a toolbox for the design of highly customizable antireflective layers. Potential fields of use include photodetectors, photoelectrochemistry, photocatalysis and general microoptoelectromechanical (MOEMS) systems.
Corpus-Based Analysis of Online Hoax Discourse on Transportation Subject Picturing Indonesian Issue
Authors: Pinem Yune Andryani 
Published: October 2021

This research discussed Indonesian hoax on transportation, spread using chain messages over social media, a major hoax subject. Setting social structure as an element to consider in hoax discourse gave different perspectives on why hoax news took place. Contrasting identified online hoax discourse to corpus-derived prosody could be interpreted using a sociopragmatic approach by Lin and Chung (2016). This research tried to look at how discourse on hoax correlated to real social issues among Indonesian people. Digital forensic was done by tracking discourse used as data from hoax news collection on turnbackhoax.com during 2017-2019. Discourse was later generated into clusters of subject idea of ‘tilang’, ‘jalan tol’, public land transport and infrastructure, ‘kecelakaan pesawat’, ‘lowongan kerja’, ‘China’, insecurity toward government and ‘kecelakaan kapal’. The subject idea was later consulted to Indonesian Web Corpus (IWAC). Through the socio-pragmatic approach, prosody derived by contrasting process portrayed hoax on transportation as a reflection of unsolved current social problems among Indonesian community including distrust toward the government, unawareness on transport safety, law and regulation, economic status, and trend. The finding of this research is recommended to take action toward those problems to eliminate hoax spread in the transportation sector.
New Hope for Intervertebral Disc Degeneration: Bone Marrow Mesenchymal
Stem Cells and Exosomes Derived from Bone Marrow Mesenchymal
Stem Cell Transplantation
Authors: Zhang Xiao-bo , Chen Xiang-yi , Qi Jin , Zhou Hai-yu , Zhao Xiao-bing , Hu Yi-cun , Zhang Rui-hao , Yu De-chen , Gao Xi-dan , Wang Ke-ping , Ma Lin 
Published: October 2021

:
Bone Marrow Mesenchymal Stem Cells (BMSCs), multidirectional cells with self-renewal
capacity, can differentiate into many cell types and play essential roles in tissue healing and regenerative
medicine. Cell experiments and in vivo research in animal models have shown that BMSCs
can repair degenerative discs by promoting cell proliferation and expressing Extracellular Matrix
(ECM) components, such as type II collagen and protein-polysaccharides. Delaying or reversing
the Intervertebral Disc Degeneration (IDD) process at an etiological level may be an effective
strategy. However, despite increasingly in-depth research, some deficiencies in cell transplantation
timing and strategy remain, preventing the clinical application of cell transplantation. Exosomes exhibit
the characteristics of the mother cells from which they are secreted and can inhibit Nucleus
Pulposus Cell (NPC) apoptosis and delay IDD through intercellular communication. Furthermore,
the use of exosomes effectively avoids problems associated with cell transplantation, such as immune
rejection. This manuscript introduces almost all of the BMSCs and exosomes derived from
BMSCs (BMSCs-Exos) described in the IDD literature. Many challenges regarding the use of cell
transplantation and therapeutic exosome intervention for IDD remain to be overcome.
The Effects of Human Umbilical Cord Mesenchymal Stem Cell Transplantation
on Female Fertility Restoration in Mice
Authors: Zhang Junhui , Wang Jinjuan , Liu Qiwei , Tang Yong , Ma Yuanyuan , Xue Zhigang 
Published: October 2021

Background:
Female fertility refers to the capacity to produce oocytes and achieve fertilization
and pregnancy, and it is impaired by age, disease, environment and social pressure. However,
no effective therapy that restores female reproductive ability has been established. Mesenchymal
Stromal Cells (MSCs) exhibit multilineage differentiation potential and have attracted considerable
attention as a tool for restoring female fertility.

Methods:
This study used human umbilical cord-MSCs (Huc-MSCs) to restore fertility in aging female
mice and mice with chemotherapy-induced damage through the rescue of ovarian function
and reconstruction of the fallopian tubes and uterus. In our study, two mouse models were generated:
aging mice (35 weeks of age) and mice with chemotherapy-induced damage.

Results:
The effect of MSCs on the ovaries, fallopian tubes and uterus was evaluated by analyzing
gonadal hormone levels and by performing morphological and statistical analyses. The levels of estradiol
(E2) and follicle-stimulating hormone (FSH) exhibited significant recovery after Huc-MSC
transplantation in both aging mice and chemotherapy-treated mice. Huc-MSC treatment also increased
the number of primordial, developing and preovulatory follicles in the ovaries of mice.
Moreover, MSCs were shown to rescue the morphology of the fallopian tubes and uterus through
mechanisms such as cilia regeneration in the fallopian tubes and reformation of glands and endometrial
tissue in the uterus.

Conclusion:
Huc-MSCs may represent an effective treatment for restoring female fertility through
recovery from chemotherapy-induced damage and rescue of female reproductive organs from the
effects of aging.
Treponema pallidum (Syphilis) Antigen TpF1 Induces Activation of
Macrophages and Accelerates P2X7R-Induced NLRP3-Dependent Release
of IL-1β
Authors: Lu Dong-Ping , Jia Jie , Wei Shao-Feng , Zhang Wei-Lian , Liang Rui , Liu Ting , Yang Wen-Zhi , Li Bing-Yan , Zhang Rong , Wang Fen 
Published: October 2021

Background:
Syphilis is a chronic infectious disease caused by Treponema pallidum (Tp)
infection, which causes local inflammation in the host. TpF1 is an oligomeric protein expressed
by the Tp-infected host that can induce the host immune response. There are few studies regarding
the role of TpF1 in macrophage activation and the subsequent release of cytokines.

Objective:
The objective of this study is to elucidate the effects of TpF1 on the pathological process
of Syphilis. In addition, we explored how purinergic 2X7 (P2X7R) induced NOD-like receptor
family protein 3 (NLRP3) -dependent release of interleukin-1β (IL-1β) and the underlying
mechanisms.

Methods:
We explored the influence of TpF1 on cytokine release by macrophages using qRT-PCR
and ELISA. The specific phenotype of activated macrophages was determined by flow cytometry.

Results:
TpF1 was able to activate macrophages and induce the M1 macrophage phenotype. Moreover,
TpF1 activated the NLRP3 inflammasome in macrophages, which was mediated by P2X7R.

Conclusions:
The Tp-induced protein TpF1 is able to induce macrophage activation and P2X7R-induced
NLRP3-dependent release of IL-1β. Our findings provide a theoretical basis for clarifying
the clinical symptoms and pathogenesis of syphilis.
Effect of Curcumin Suspension and Vitamin C on Dentin Shear Bond Strength and Durability. A Pilot Study
Authors: Abuelenain Dalia A. , Abou Neel Ensanya A. , Abuhaimed Tariq S. , Alamri Amal M. , Ammar Hanan S. , Bukhary Sahar M. N. 
Published: October 2021

Background:
Preserving the strength and durability of dentin bonds is a concern of dental researchers and practitioners.

Objective:
The aim of this study was to investigate the effect of treating etched dentin with vitamin C and curcumin suspension as cross-linking agents on the shear bond strength and durability of a universal dental adhesive.

Methods:
Extracted human third molar teeth were collected. Fifty-six flat coronal dentin surfaces were divided equally into four groups: (1) treated with adhesives; (2) etched with 37% H3PO4 for 10s before application of adhesives; (3) same as group (2), but with dentin treated with 2.5% curcumin suspension for 60s before the application of adhesive; and (4) same as group (3), but with the use of a 20% vitamin C suspension instead of a curcumin suspension. Filtek Z350XT composite was built for all samples. Half of the samples in each group were subjected to thermocycling. Shear bond strength was measured using a universal testing machine. Scanning electron microscopy was used to investigate the dentinal surfaces after treatment.

Results:
Treatment with curcumin suspension preserved immediate and long-term bond strength. Conversely, treatment of etched dentin with vitamin C solution significantly reduced the immediate bond strength, but a significant improvement was observed after aging. SEM showed that both cross-linking agents resulted in narrowing or closing of the dentinal tubules, with an adhesive mode of failure.

Conclusion:
The application of curcumin or vitamin C suspension to acid-etched dentin resulted in different effects on immediate and long-term shear bond strength when using universal adhesives.
Encouraging the Rise of Young Entrepreneurs: Promoting Entrepreneurial Intention through Storytelling
Authors: Taufik Taufik , Ernawati Ernawati 
Published: October 2021

Aim:The aim of this study is to use storytelling as a technique to develop entrepreneurial intention in Islamic boarding school graduates.Background:Most of thepesantrengraduates in Indonesia do not proceed on to university studies after completing their schooling due to financial problems and so lack the skills to work on jobs that could support their livelihood. Therefore,pesantrenwanted to develop a strategy to help its alumni find employment after completing their education. Increasing the entrepreneurial intention of graduates in various sectors is one way in whichpesantrenexpect to support graduates in finding their livelihood.Objective:The objective of this study is to test the effectiveness of storytelling in increasing entrepreneurial intentions in students.Methods:Sixty Islamic boarding school students participated in this study. The Solomon three-group design was used to test the effectiveness of storytelling in increasing the entrepreneurial intentions of students. Participants were divided into three groups: the experimental group, control group 1, and control group 2, each consisting of 20 students. The entrepreneurial-intention scale was used to measure the level of entrepreneurial intention among each group of students.ResultsFirst, there was a significant difference between the post-test score of entrepreneurial intention in the experimental group (with treatment) and the post-test score of entrepreneurial intention in control group 1 (without treatment). Second, the post-test score of entrepreneurial intention in the experimental group (with treatment) was not different from the post-test score of entrepreneurial intention in control group 2 (with treatment). Thus, the results were consistent in the sense that the group that received treatment had an increased level of entrepreneurial intention when compared with the group that did not receive any treatment.Conclusion:The results showed that storytelling was effective in increasing the entrepreneurial intention of students. In this study, the treatment of storytelling was integrated with a focus group discussion. Developing treatment with other techniques can have a greater effect on increasing the entrepreneurial intention of students.