bluuebunny/crossref_metadata_2025_split huggingface dataset:
TERT Promotes Neurogenesis and Neural Repair after Hypoxic Ischemic Brain Damage in Neonatal Rats
Authors: Li Jiao , Liu Hai-ting , Zhao Jing , Fang Zhi 
Published: May 2021

Abstract
Neonatal hypoxic ischemic encephalopathy (HIE) endangers quality of life in children; but effective cure is rare. Neurogenesis plays an important role in neural repair following brain damage. Recent studies have demonstrated that telomerase reverse transcriptase (TERT) was involved in neurogenesis regulation. However, whether TERT participates in the regulation of neurogenesis after hypoxic-ischemic brain damage (HIBD) is unclear. Here, we established a model of HIBD in neonatal rats both in vivo and in vitro, and used lentivirus and adenovirus transfection for TERT overexpression to investigate its role in neurogenesis after HIBD in developmental stage. Using immunofluorescence staining, cell counting Kit-8 staining, TUNEL, and western blotting, we observed that TERT attenuated apoptosis and promoted proliferation, migration, and differentiation in neural stem cells (NSCs). Furthermore, TERT induced myelination in the brain of neonatal rats after HIBD. Neurobehavioral tests revealed that TERT could improve learning, memory, and neurological function after HIBD in neonatal rats, and thus promote the recovery of neurological function after HIBD. In addition, we investigated the regulatory mechanism of TERT during neurogenesis after HIBD in developmental stage. We found that TERT may regulate neurogenesis after HIBD through the Sonic Hedgehog/Gli1 signaling pathway. Our study demonstrated that TERT could promote neural repair and neurological function recovery after HIBD in neonatal rats. The new neuroprotective pathway regulated by TERT during HIBD described here could provide a basis for developing therapeutic strategy for neonatal HIE. Furthermore, TERT may be a potential target during neural repair and reconstruction in various diseases affecting nervous system.
Taking better advantage of fold axis data to characterize anisotropy of complex folded structures in the implicit modeling framework
Authors: Pizzella Laure , Alais Robin , Lopez Simon , Freulon Xavier , Rivoirard Jacques 
Published: May 2021

Abstract
When too few field measurements are available for the geological mod-ling of complex folded structures, results of implicit methods typically exhibit an unsatisfactory bubbly aspect. However, in such cases, anisotropy data are often readily available but not fully exploited. Among them, fold axis data are a straightforward indicator of this local anisotropy direction. Focusing on the so-called potential field method, this work aims to evaluate the effect of the incorporation of such data into the modeling process. Given locally sampled fold axis data, this paper proposes to use the second-order derivatives of the scalar field in addition to the existing first-order ones. The mathematical foundation of the approach is developed and the respective efficiencies of both kinds of constraints are tested. Their integration and impact are discussed based on a complex case study, thereby providing practical guidelines to geomodeling tool users on the parsimonious use of data for the geological modeling of complex folded structures
Characterization of the Physical Origins of Acoustic Emission (AE) From Natural Fiber Reinforced Polymers (Nfrps) Machining Processes
Authors: Wang Zimo , Guo Ruiqi , Ma Qiyang , Chegdani Faissal , Tai Bruce , Mansori Mohamed El , Bukkapatnam Satish 
Published: May 2021

Abstract
Natural fiber reinforced polymers (NFRPs) are environmentally friendly and are receiving growing attention in the industry. However, the multi-scale structure of natural fibers and the random distribution of the fibers in the matrix material severely impede the machinability of NFRPs, and real-time monitoring is essential for quality assurance. This paper reports a synchronous in-situ imaging and acoustic emission (AE) analysis of the NFRP machining process to connect the temporal features of AE to the underlying dynamics and process instability, all happen within milliseconds during the NFRP cutting. This approach allows directly observing the surface modification and chip formation from a high-speed camera (HSC) during NFRP cutting processes. The analysis of the HSC images suggests that the complex fiber structure and the random distribution introduce an unsteady, almost a freeze-and-release type motion pattern of the cutting tool with varying depths of cut at the machining interface. More pertinently, a prominent burst pattern of AE from time domain was found to emanate due to the sudden penetration of the tool into the surface of the NFRP workpiece (increasing the depth of cut), as well as a release motion of the tool from its momentary freeze position. These findings open the possibility of tracking AE signals to assess the effective specific energy and surface quality that are affected by these unsteady motion patterns.
Ropivacaine induces cell cycle arrest in the G0/G1 phase and apoptosis of PC12 cells via inhibiting mitochondrial STAT3 translocation
Authors: Zeng Lian , Zhang Zhen , Zhang Fuyu , Chen Huaxian , Wang Ying , Ding Xudong , Luo Huiyu 
Published: May 2021

Abstract
STAT3 has neuroprotective effect via non-canonical activation and mitochondrial translocation, but its effects on ropivacaine-induced neurotoxicity remain unclear. Our previous study revealed that apoptosis was an important mechanism of ropivacaine-induced neurotoxicity, this study is to illustrate the relationship between STAT3 with ropivacaine-induced apoptosis. Those results showed that ropivacaine treatment decreased cell viability, induced cell cycle arrest in the G0/G1 phase, apoptosis, oxidative stress, and mitochondrial dysfunction in PC12 cells. Besides, ropivacaine decreased the phosphorylated levels of STAT3 at Ser727 and downregulated the expression of STAT3 upstream gene IL-6. The mitochondrial translocation of STAT3 was also hindered by ropivacaine. To further illustrate the connection of STAT3 protein structure with ropivacaine, the autodock-vina was used to examine the interaction between STAT3 and ropivacaine, and the results showed that ropivacaine could bind to STAT3’s proline site and other sites. In addition, the activator and inhibitor of mitoSTAT3 translocation were used to demonstrate it was involved in ropivacaine-induced apoptosis, the results showed that enhancing the mitochondrial STAT3 translocation could prevent ropivacaine-induced apoptosis. Finally, the expression of p-STAT3 and the levels of apoptosis in the spinal cord were also detected, the results were consistent with the cell experiment, ropivacaine decreased the expression of p-STAT3 protein and increased the levels of apoptosis in the spinal cord. We demonstrated that ropivacaine induced apoptosis by inhibiting the phosphorylation of STAT3 at Ser727 and the mitochondrial STAT3 translocation. This effect was reversed by the activation of the mitochondrial STAT3 translocation.
Accuracy of Robot-Assisted Percutaneous Pedicle Screw Placement Under Regional Anaesthesia: A Comparative Cohort Study
Authors: Gao Shangju , Wei Jingchao , Li Wenyi , Zhang Long , Cao Can , Zhai Jinshuai , Gao Bo 
Published: May 2021

Abstract
Background: Robot-assisted pedicle screw placement is usually performed under general anaesthesia to keep the body still. The aim of this study was to compare the accuracy of the robot-assisted technique under regional anaesthesia with conventional fluoroscopy-guided percutaneous pedicle screw placement under general anaesthesia in minimally invasive lumbar fusion surgery.Methods: Patients who underwent robot-assisted percutaneous endoscopic lumbar interbody fusion (PELIF) or fluoroscopy-guided minimally invasive transforaminal lumbar interbody fusion (MIS-TLIF) from December 2017 to February 2020 in a single centre were included. Based on the method of percutaneous pedicle screw placement used, patients were divided into the robot-assisted under regional anaesthesia (group RE-RO) and fluoroscopy-guided under general anaesthesia (group GE-FLU) groups. The primary outcome measures were screw accuracy and the incidence of facet joint violation (FJV). Secondary outcome measures included X-ray exposure and intraoperative adverse events.Results: Eighteen patients were included in group RE-RO, and 23 patients were included in group GE-FLU. The percentages of clinically acceptable screws (Gertzbein and Robbins grades A and B) were 94.4% and 91.5%, respectively. There was no significant difference in the percentages of clinically acceptable screws (p=0.44) or overall Gertzbein and Robbins screw accuracy grades (p=0.35). Only the top screws were included in the analysis of FJVs. The percentages of FJV (Babu grades 1, 2 and 3) were 5.6% and 28.3%, respectively. This difference was statistically significant (p=0.01). Overall, the FJV grades in group RE-RO were significantly better than those in group GE-FLU (p=0.009). The mean fluoroscopy time for each screw in group RE-RO was significantly shorter than that in group GE-FLU (group RE-RO, 5.4±1.9 seconds, group GE-FLU, 6.8±2.0 seconds; P=0.03). The intraoperative adverse events included 1 case of registration failure and 1 case of guide-wire dislodgment in group RE-RO as well as 2 cases of screw misplacement in group GE-FLU. No complications related to anaesthesia were observed.Conclusion: Robot-assisted pedicle screw placement under regional anaesthesia can be performed effectively and safely. The accuracy is comparable to the conventional technique. Moreover, this technique has the advantage of fewer FJVs and a lower radiation time.
The Adaptation of Turkish Health Literacy Scale for Literate Syrian Adult Refugees Living in Turkey: A Reliability-Validity Study
Authors: Bahar-Ozvaris Sevkat , Guciz-Dogan Bahar , Unlu Hande Konsuk , Aktuna Gamze , Inandi Tacettin , Kurt A. Oner , Aydin Neriman , Kutluk M. Tezer 
Published: May 2021

Abstract
BackgroundThere is a big Syrian refugee population which counts more than 3.5 million since the year 2011, and continuously growing. This situation causes various problems, mainly while obtaining health services. In planning the migrant health services, for the policy makers of host countries, health literacy level of migrants is an important measure. Determination of health literacy level of Syrian refugees in Turkey would be supportive for planning some interventions to increase health services utilization, and health education and health communication programs. An “original health literacy scale" for 18–60 years of age Turkish literate adults (Hacettepe University Health literacy Scale-HLS) was developed to be used as a reference scale in 2018. It would be useful to compare the health literacy level of Turkish adults and Syrian adult refugees resided in Turkey with an originally developed scale. For this reason, it was aimed to adapt the HLS-Short Form in Syrian refugees.MethodsThis methodological study was carried out between 2019–2020 in three provinces of Turkey where the Syrians live intensively. The data was collected by pre-trained, Arabic speaking 12 interviewers and three supervisors via a questionnaire on household basis. At first, the original Scale and questionnaire were translated into Arabic and backtranslated. The questionnaire and the Scale was pre-tested on 30 Syrian refugees in Ankara Province. A total of 1254 refugees were participated to the main part of study; 47 health-worker participants were excluded from the validity-reliability analysis. Confirmatory factor analysis (CFA) was performed. Cronbach’s alpha and Spearman-Brown coefficients were calculated.ResultsOf the participants, 52.9% was male; 26.1% had secondary level or less; almost half of them had moderate economic level; 27.5% could not speak Turkish. The Cronbach’s Alpha was 0.75, Spearman-Brown Coefficient was 0.76; RMSEA = 0.073, CFI = 0.93, TLI = 0.92 and GFI = 0.95 for the Scale. The Cronbach’s Alpha was 0.76, Spearman-Brown Coefficient was 0.77; RMSEA = 0.085, CFI = 0.93, TLI = 0.91 and GFI = 0.95 for self-efficacy part.ConclusionIn conclusion, the adapted HLS would be a reliable instrument to evaluate the health-literacy level of Syrians living in Turkey and could give an opportunity to compare the host country’s HL with the refugees by using the same scale.
Propofol Improves Survival In A Murine Model of Sepsis Via Inhibiting Rab5a -Mediated Intracellular Trafficking of TLR4
Authors: Zhou Bo-Wei , Zhang Fang-Ling , Zhang Wen-Juan , Yang xiao , Yao Zhi-Wen , Yan Zheng-Zheng , Zhao Bing-Cheng , Zhao Jin , Feng Wei-Jie , Chen Xiao-Dong , Li Cai , Liu Ke-xuan 
Published: May 2021

Abstract
Background：Propofol is a widely used anesthetic and sedative, which has been reported to exert an anti-inflammatory effect. TLR4 plays a critical role in coordinating the immuno-inflammatory response during sepsis. Whether propofol can act as an immunomodulator through regulating TLR4 are still unclear. In view of its potential as a sepsis therapy, we investigated the mechanisms underlying the immunomodulatory activity of propofol.Methods: The effects of propofol on TLR4 and Rab5a (a master regulator involved in intracellular trafficking of immune factors) were investigated in macrophage (from Rab5a-/- and WT mice) following treatment with lipopolysaccharide or cecal ligation and puncture in vitro and in vivo, and in peripheral blood monocyte from sepsis patients and healthy volunteers. Results: We showed that propofol reduced membrane TLR4 expression on macrophage in vitro and in vivo. Rab5a participated in TLR4 intracellular trafficking and both Rab5a expression and the interaction between Rab5a and TLR4 were inhibited by propofol. We also showed Rab5a upregulation in peripheral blood monocyte of septic patients, accompanied by increased TLR4 expression on the cell surface. Both were correlated with SOFA score of sepsis patients and higher expression of Rab5a were found in septic non-survivors. Propofol downregulated the expression of Rab5a and TLR4 in these cells.Conclusions：We demonstrated that Rab5a regulates intracellular trafficking of TLR4 and that propofol reduces membrane TLR4 expression on macrophages by targeting Rab5a. Our study not only reveals a novel mechanism for the immunomodulatory effect of propofol but also indicates that Rab5a may be a potential therapeutic target against sepsis.
Exploring Vertebrate Behavior at Mesocarnivore Carcass Sites: Implications for Non-Trophic Parasite Transmission
Authors: Moisés Gonzálvez , Martínez-Carrasco Carlos , Marcos Moleón 
Published: May 2021

Abstract
The spatiotemporal distribution of parasites in ecosystems is heterogeneous. High infection risk is often associated with aggregations of animals around attractive resources. Here, we explore the behavior of potential hosts of non-trophically transmitted parasites at mesocarnivore carcass sites. We used videos recorded by camera traps at 56 red fox (Vulpes vulpes) carcasses and 10 carcasses of other wild carnivore species in three areas of southeastern Spain. In general, scavenging species, especially wild canids, mustelids and viverrids that display rubbing behavior, were more exposed to infection risk at carnivore carcass sites than non-scavenging and domestic species. The red fox was the species that most frequently contacted carcasses and marked and rubbed carcass sites. Foxes contacted heterospecific carcasses more frequently and earlier than conspecific ones, and close contact was more frequently observed at heterospecific carcasses. Thus, foxes seemed to avoid contacting carcasses in those periods and at carcass types of maximum risk of acquiring parasites. Overall, our results suggest that infection risk at carnivore carcass sites may take place mainly for visitor-carcass contact or contact with feces and urine rather than direct contact between visitors. Moreover, contact events between scavengers and carnivore carcasses are far more frequent than consumption events, which indicates that scavenger behavior is more constrained by the risk of acquiring meat-borne parasites than non-trophically transmitted parasites. This study contributes to filling key gaps in understanding the role of carrion in the landscape of disgust, which may be especially relevant in the current global context of emerging and re-emerging pathogens.
Soybean responses to nutrients deficiency, and the possibility of detecting this deficiency using chlorophyll fluorescence technique
Authors: Latifinia Elham , Eisvand Hamid Reza 
Published: May 2021

Abstract
Aims Managing plant nutrition is a key factor to getting optimum yield quantity and quality. Soybean is an important plant as an oil and protein producer crop as well as a biological nitrogen fixing plant. The aims of current work were studying soybean's responses to some macro and micronutrients deficiency stress as well as the possibility of diagnosing this deficiency using chlorophyll fluorescence technique.Methods The two-year field experiment during 2019 and 2020 growth seasons were conducted based on a randomized complete block design with three replications. Treatments were use and non-use of N, P, Fe, and Mo, accompanied with and without humic acid. N and P were applied in the soil, but Fe, Mo, and humic acid were foliar applied at the final vegetative growth stage. Results Results showed that the effect of fertilizer treatments was significant on all traits. N-P-Fe-Mo improved grain yield and photosynthesis rate, but their application accompanied with humic acid induced a synergistic effect, and the maximum grain yield and photosynthesis were recorded in the N-P-Fe-Mo + HA. Fertilizer application decreased F0 and Fm and increased Fv/Fm. Besides, there was a significant negative correlation between leaf's N, P, Fe, and Mo content with Fm; meanwhile, the negative correlation between leaf's nitrogen and Fm was stronger than the other applied nutrients (r=-0.767). Conclusions Research findings show that, it is possible to use the chlorophyll fluorescence technique as a valid non-destructive physiological indicator and a quick way to monitor the nutritional status of soybean plant about N-P-Fe-Mo to timely fertilizing. Although soybean is a nitrogen fixing plant, but it needs complementary N fertilizer to achieve maximum PSII efficiency, minimum chlorophyll fluorescence, and optimal yield.
Fine-Tuning Dye Adsorption Capacity of UiO-66 by Mixed-Ligand Approach
Authors: Nanthamathee Chompoonoot , Chantarangkul Chantamalinee , Jakkrawhad Chanida , Payaka Apirak , Dechatiwongse Pongsathorn 
Published: May 2021

Abstract
Mixed ligand synthetic approach offers an alternative to engineer a specific character in metal-organic framework (MOFs) materials. Herein, we synthesized and characterized a well-known prototype zirconium based-MOF, so called UiO-66 and its mixed ligand derivatives UiO-66-xATA, where x is mole fraction (0.5, 0.75 and 1.0) and ATA is 2-animoterephthalate. The aim of the study is to observe whether the dye adsorption capacity can be tuned/enhanced by the ATA ligand substitution into the framework. We found that, at room temperature, UiO-66-0.75ATA shows the highest adsorption capacity toward various dye solution including methylene blue (MB), indigo carmine (IC) and congo red (CR). The optimum adsorption conditions in all four materials were found to be in a common trend where their adsorption capacities can be increased with decreasing pH and adsorbent dose, increasing of IC concentration, contact time and temperature. Pseudo-second order kinetics model fits best with their adsorption data, where UiO-66-ATA has the fastest rate of adsorption. Langmuir and Freundlich isotherms were found to best describe adsorption behaviour in ATA-containing UiO-66 and UiO-66 respectively, where adsorption processes were found to be physisorption. Confirming by thermodynamic studies, the adsorption in all four materials occurred spontaneously, driven by entropy. Computational studies showed ligand to metal charge transfer where the distribution of electron densities was varied with the amount of functionalized ligand. Adsorption mechanism is proposed as a synergistic interplay between electrostatic interaction and hydrogen bonding. The findings in this work broaden potential strategy to fine-tune the dye adsorption capacity in MOF materials.
Usefulness of Matrix Metalloproteinase-7 in Saliva as a Diagnostic Biomarker for Laryngopharyngeal Reflux Disease.
Authors: Im Nu-Ri , Kim Byoungjae , Jung Kwang-Yoon , Baek Seung-Kuk 
Published: May 2021

Abstract
Introduction

Several diagnostic methods are currently being used to diagnose LPRD (laryngopharyngeal reflux disease), but have the disadvantage of being invasive, subjective, or expensive.
Objectives

Our purpose in this study was to investigate the correlation between pepsin and MMP-7 (Matrix Metalloproteinase-7) in pharyngeal secretions of subjects according to RSI (Reflux Symptom Index) score to find out the diagnostic value of MMP-7.
Method

We recruited 173 subjects aged between 19 and 85 years who completed the RSI scale. All samples were taken after waking up, and the amount of the pepsin and MMP-7 in saliva were measured by means of an enzyme activity assay.
Results

There was a significant increase of pepsin and MMP-7 activity in the study group with an RSI score of 13 or higher. The sensitivity and specificity of MMP-7 for predicting the possibility of an RSI of 13 or more was higher than that of pepsin. When MMP-7 and pepsin were combined, this sensitivity and specificity increased.
Conclusion

An enzyme assay of MMP-7 in saliva may be a noninvasive and easy technique for diagnosing LPRD.
IgG antibody response against Nucleocapsid and Spike protein post SARS-CoV-2 infection
Authors: Choudhary Hari Ram , Parai Debaprasad , Dash Girish Chandra , Peter Annalisha , Sahoo Subrat Kumar , Pattnaik Matrujyoti , Rout Usha Kiran , Nanda Rashmi Ranjan , Pati Sanghamitra , Bhattacharya Debdutta 
Published: May 2021

Abstract
Objectives: Coronavirus disease-19 (COVID-19) pandemic became the greatest public health challenge globally. Study of dynamicity and durability of naturally developed antibodies against SARS-CoV-2 are of great importance from an epidemiological viewpoint.Methods: In this observational cohort study, we have followed up the 76 individuals who tested positive for SARS-CoV-2 infection by real-time reverse transcriptase-polymerase chain reaction (RT-qPCR) for 16 weeks (post enrollment) to record the periodic changes in titre, concentration, clinical growth and persistence of naturally developed SARS-CoV-2 antibodies. We collected serum samples from these individuals for 16 weeks with a frequency of weekly and fortnightly during each follow-up and tested them in two CLIA-based platforms (Abbott Architect i1000SR and Roche Cobas e411) for testing SARS-CoV-2 antibodies both qualitatively and quantitatively.Results: We recorded the antibody magnitude of these individuals 10 times between September 2020 and February 2021. We found a waning of antibodies against nucleocapsid antigen protein but not a complete disappearance by the end of 16 weeks. Out of 76 cases, 30 cases (39.47%) became seronegative in qualitative assay, although all the sera samples (100%) remained positive when tested in quantitative assay.Conclusion: The lower persistence of anti-nucleocapsid SARS-CoV-2 antibody may not be the exact phenomenon as those cases were still seropositive against spike protein and help in neutralizing the virus.