bluuebunny/crossref_metadata_2025_split huggingface dataset:
Improving the integrated pastoral resource management of SAPARM information system using Landsat NDVI value for mobility decision making in Lege-Hidha district; Bale lowlands, Southeast Ethiopia
Authors: Meshageria Sintayehu , Alemu Mersha , Legesse Behailu 
Published: January 2021

Abstract
Availability of pasture and water are essential factors determine time and direction of pastorals movement. In Ethiopian pastorals mobility is a common coping strategy for dealing drought and its induced risks. Helping pastoralists more informed on decisions to manage their resource reduces response costs and livelihood losses. Matching scientiﬁc systems with traditional knowledge can lead to the successful resource managements. The aim of this study is improving the Integrated Pastoral Resource Management of SAPARM Information System using Landsat NDVI Value for Mobility Decision Making. The study was conducted on pastorals and agro-pastorals livelihood zones where livestock production is common. Data was collected in Lege-Hidha district using FGD, key informant interviews, community mapping and spatial satellite images from USGS. The spatial data was analysed using GIS/RS spatial data analysis tools. The data analysis on improving the integrated pastoral resource management (SAPARM and traditional system) using Landsat NDVI value is derived from USGS; Landsat at Path 166/167 and Row 54/55 (study area location found between 166/54, 166/55 and 167/54, 167/55) that verified and visualized using ArcGIS 10.3 and Google earth in order to compare Landsat8 NDVI values of 30m2 resolutions with SAPARM information from Meteosat NDVI at 10km2. Landsat8 analysis confirmed that areas where mobility is conducted have better and detailed vegetation (greenness) of enhanced reflectance than SAPARM. This was due to Landsat resolution capacity provides visible and detailed images of the invisible reflectance area on SAPARM, which improves pastoral mobility and decisions based on distances, direction, greenness, classification and allow knowing immediately specific places. Integration systems in this study attempted to apply traditional resource management with satellite assisted information using images of better resolution capacity enables to clarify and detailed reflectance. The improvement using NDVI values of Landsat ensure images with intensive areas of vegetation cover than Meteosat images of SAPARM.
The Effect of MS strength, pH and Sucrose Concentrations on In vitro Propagation of Okra (A belmoschus esculentus L.) from&nbsp;Shoot Tip Explants .
Authors: Woldeyes Girma Gebrehiwot , Senbeta Tileye Feyissa , Adugna Alelegne Yeshamebel , Abegaz Agegnehu Wasse 
Published: January 2021

Abstract
BackgroundOkra (Abelmoschus esculentus) is belongs to the family Malvaceae and genus Hibiscus. Conventional propagation of okra in a large scale is limited due to bacterial, fungal, viral disease and seed dormancy. Hence, micropropagation offers great potential to increasing the commercial availability of okra. This study aimed to investigate the effect of MS strength, sucrose concentration, and pH on the in vitro propagation of A. esculentus from shoot tips. ResultsOkra seeds were sterilized with 70% alcohol, 30% (v/v) Berekina and 0.13% HgCl2 (w/v) for 5, 40 and 10 minutes respectively. The shoots were excised from in vitro germinated seedlings and transferred to full strength MS liquid medium containing 1mg/L BAP. The in vitro initiated shoot tips were transferred to different MS strength (1, ½, ⅓, ¼) medium, sucrose concentration (30 g/L, 20 g/L, 15 g/L, 10 g/L) and pH level (pH 6.6, 6.2, 5.8, 5.4, 5.0) that contained 1.5 mg/L BAP and 200 mg/L activated charcoal. For rooting, different MS strength (full, ½, ⅓, ¼), sucrose (30 g/L, 20 g/L, 15 g/L and 10 g/L) and pH (6.6, 6.2, 5.8, 5.4, 5.0) that contained 1mg/L IBA with 200 mg/L activated charcoal were used. Full strength MS medium containing 30 g/L sucrose at pH 5.8 produced the highest mean shoot number (7.93), mean leaf number (14.37) and mean length (6.4 cm) per explant. Similarly, at pH 6.2 and 6.6 maximum shoot mean number (6.53, 5.80), leaf number (12.77, 10.43) and shoot length (6.32, 5.82 cm) were produced respectively. Maximum mean root number (15.73) and root length (6.05 cm) were recorded at ½ MS strength. At pH 5.8, 6.2 and 6.6 maximum mean root number (1.77, 1.47, 1.80) and root length (1.38, 1.25, 1.39 cm) were obtained. After acclimatization, 86% of plants survived in greenhouse. ConclusionDecreasing the MS strength, sucrose concentration and pH level had an inhibiting effect on both shoot multiplication and root formation of okra. Besides increasing pH value showed inhibiting effect on shoot multiplication.
MicroSEC: Sequence error filtering pipeline for formalin-fixed and paraffin-embedded samples
Authors: Ikegami Masachika , Kohsaka Shinji , Hirose Takeshi , Ueno Toshihide , Kanomata Naoki , Yamauchi Hideko , Kobayashi Hiroshi , Tanaka Sakae , Mano Hiroyuki 
Published: January 2021

Abstract
The clinical sequencing of tumors is usually performed on formalin-fixed, paraffin-embedded (FFPE) samples and results in many sequencing errors. Most of these errors are detected in chimeric reads caused by single-strand DNA molecules with microhomology. Our filtering pipeline, MicroSEC, focuses on the uneven distribution of mutations in each read and removes the sequencing errors in FFPE samples without eliminating the true mutations that are also detected in fresh frozen samples.
HPV16 E6-178G/E7-647G Promotes Proliferation and Inhibits Apoptosis in Cervical Cancer C33A Cells
Authors: Zhe Xiangyi , Zhang Chunhe , Xin Huizhen , Pan Zhenzhen , Li Dongmei , Li Hongtao , Jin Fuyuan , Long Haichen , Zheng Weinan , Shao Renfu , Pan Zemin 
Published: January 2021

Abstract
BackgroundHPV16 is the main cause of cervical cancer. In our study, we aimed to investigate the role of HPV mutants HPV16 E6-178G/E7-647G in the proliferation and apoptosis of cervical cancer C33A cells. MethodsPlasmids encoding the HPV16 E7 prototype (E7-647A)-GV144, E7 mutant (E7-647G)-GV144, HPV16 E6/E7 prototype (E6-178T/E7-647A)-GV144, and E6/E7 mutant (E6-178G/E7-647G)-GV144 were stably transfected into cervical cancer C33A cells. Western blot analysis, CCK8 proliferation assay, cell cloning assay and flow cytometry were used to detect the effects of the different polymorphism sites in HPV16 on cell proliferation and apoptosis. ResultsHPV16 mutations promoted the proliferation and inhibited the apoptosis of cervical cancer C33A cells, and the effect of the E6-178G/E7-647G co-mutation was significantly greater than that of the single E7-647G mutant (P<0.05). ConclusionsHPV16 E6-178G/E7-647G can thus promote the proliferation and inhibit the apoptosis of cervical cancer cells. Importance: HPV16 is the most common type and the main cause of cervical cancer. It is a fatal threat to the health of millions of women. The genetic differences among HPV16 sub-types may be related to their carcinogenic potential. HPV mutations can differ in biology and etiology, leading to differences in tumor development and behavior. However, little is known about the carcinogenic potential of the HPV16 variant in Asian women compared to many studies in European and American populations.
The Surface Antigen 1(SAG1) of Toxoplasma Gondii Interacts With RACK1 Inducing Autophagy and Maintaining The Viability of Host Cells
Authors: An Ni , Li Bing , Liu Yu-ming , Zhang Zhi-xuan , Su Rongjian , Liu Xiao-gang 
Published: January 2021

Abstract
BackgroundIt has been well known that the surface antigen 1(SAG 1) of T.gondii plays an important role in the invasion of Tachyzoite into host cells. However whether it also play a role in the intracellular parasitism of T.gondii remains unclear. The main purpose of this study was to determine the effect of SAG1 on host cells and investigate the underlying mechanism. MethodsSAG1 was overexpressed in human embryonic kidney cell 293(HEK) by transfection. Autophagy was determined by fluorescent microscope and flow cytometry (FCM) in HEK293 cells co-transfected with Flag-SAG1 and EGFP-LC3. The interaction of SAG1 and RACK1 was measured by co-immunoprecipitation(Co-IP), GST pulldown and fluorescent microscope. The expression of cytokines including IL-1β, IL-6, and IL-12 was determined by qRT-PCR. The expression of LC3, Ki67 and RACK1 was detected by Western blot. Cellular senescence was measured by β-galactosidase staining.ResultsWe found that overexpression of SAG1 in human embryonic kidney cells (HEK293) induces non-canonical autophagy and inhibition of autophagy using hydroxychloroquine (HCQ) significantly decreases the cell viability of HEK293 cells. Mechanically, we identified RACK1, an intracellular multifunctional protein as a binding partner of SAG1. Depletion of RACK1 inhibited SAG1 induces non-autophagy and decreases the enhanced expression of cytokines including IL-1β, IL-6, IL-12 and TNF-α in SAG1 overexpressing cells. ConclusionThese data showed that SAG1 could induce non-canonical autophagy and facilitate the expression of IL-1β, IL-6, IL-12 and TNF-α by interacting with RACK1, maintaining the viability of host cells. Our results suggests a new contribution of SAG1 in the intracellular parasitism.
Phased Repatriation of Lebanese Expatriates Stranded Abroad During Coronavirus Disease 2019 (COVID-19) Pandemic
Authors: Youssef Dalal , Berry Atika , Ghosn Nada , Zalzali Mahmoud , Fadlallah Riad , Abbas Linda Abou , Hassan Hamad 
Published: January 2021

Abstract
Background: The coronavirus disease (COVID-19) pandemic represents a serious worldwide threat. Stranded Lebanese citizens abroad appealed to the Lebanese government to embark on citizen repatriation missions. Objectives: We aim to document the Lebanese experience in the repatriation of citizens during COVID-19 which allow us to disclose encountered challenges and lessons learned. Methodology: This is a retrospective description of processes involved in the phased repatriation of Lebanese citizens. The Mission consisted of 4 phases starting, April 5th until June 19th 2020. The prioritization of returnees was based on both medical and social risk assessment. The repatriation team was divided into four groups: the aircraft team, the airport team, the hotel team and the follow up team. On arrival, all returning citizens were tested using Polymerase chain Reaction (PCR) based technique, and were obliged to adhere to a mandatory facility quarantine for 24 to 48 hours. Returning travelers who were tested positive for COVID-19 were transferred to the hospital. Those who were tested negative were urged to strictly comply with home-quarantine for a duration of 14 days. They were followed up on a daily basis by the repatriation team. Results: Overall, 25783 Lebanese citizens have returned home during the phased repatriation. The third phase ranked the uppermost in regard of the number of citizens repatriated. The total number of performed PCR tests at the airport upon arrival was 14893 with an average percentage of around 1% positivity for COVID-19. On the other hand, more than 10687 repatriates underwent external PCR requisite in the third and fourth phases. 272 repatriates were tested positive for COVID-19 upon their arrival. Conclusion: Considering the limited human and financial resources besides the economic and political crisis, the overall repatriation mission could be considered as a successful experience. Such processes would not have been achieved without the professionalism of all involved stakeholders.
GREB1L overexpression correlates with prognosis and immune cell infiltration in lung adenocarcinoma.
Authors: Yu Yilin , Wang Zhiping , Zheng Qunhao , Li Jiancheng 
Published: January 2021

Abstract
GREB1L is a protein-coding gene that is an important paralog of GREB1. However, its effects in lung adenocarcinoma (LUAD) have not been determined. Thus, we evaluated the prognostic value of GREB1L in LUAD using bioinformatics approaches. In particular, we evaluated the relationship between GREB1L and LUAD using a wide range of databases and analysis tools, including TCGA, GEO, HPA, TIMER, cBioPortal, and MethSurv. Compared with its expression in normal lung tissues, GREB1L expression was significantly increased in LUAD tissues. A univariate Cox analysis showed that high GREB1L expression levels were correlated with a poor OS in LUAD. Additionally, GREB1L expression was independently associated with OS through a multivariate Cox analysis. GO and GSEA revealed enrichment in cell cycle and methylation. Moreover, high GREB1L expression was associated with poor survival. We also found that the methylation and genetic alteration level was associated with prognosis in patients with LUAD. Finally, an analysis of immune infiltration showed that GREB1L is correlated with immune cell infiltration, PD-1 and PD-L1. In summary, these results indicate that GREB1L is a potential molecular marker for poor prognosis in LUAD and provide additional insight for the development of therapies and prognostic markers.
Conversation Skills in Chinese-Speaking Preschoolers with Autism: The Contributing Role of Parents’ Verbal Responsiveness
Authors: So Wing Chee , Song Xueke , Cheng Chun Ho , Law Wing Wun , Wong Tiffany , Leung Oi Ki , Huang Ying 
Published: January 2021

Abstract
Background: Children with autism spectrum disorder (ASD) have deficits in conversation skills, yet the growth of conversation abilities is understudied, especially in Chinese-speaking populations, whose autism rates are rising. Additionally, little is known about whether parents’ verbal responsiveness (utterances that follow the child’s focus of attention, actions, and communications) and redirectives (utterances that require the child to change the focus of attention) are related to their ASD children’s elicitation of responses in a conversation. This study had two aims: 1) documenting the growth of conversation skills in Chinese-speaking preschool children with autism; 2) investigating the association between parents’ verbal responsiveness and redirectives and the production of appropriate responses in the children with ASD and the number of conversational turns in the parent-child dyads. Method: Participants were taken from a larger intervention project for (Cantonese) Chinese-speaking individuals with ASD. Thirty-seven participants with ASD (32 males), average age five to six years, and their parents, contributed their language samples. These children interacted with their parents at four time points over nine months. Each time, the parents and children played with a standard set of toys for 20 minutes. The severity of autism, cognitive functioning, and language abilities of the children were assessed before the collection of their language samples. Results: Our findings showed that the number of conversational turns and the proportion of child-initiated conversation (but not the proportion of children’s appropriate responses) grew over the course of nine months. After controlling for time, autism severity, and language skills, parents’ verbal responsiveness positively predicted children’s appropriate responses. Parents’ redirectives negatively predicted the proportion of children’s appropriate responses and the number of conversational turns. Limitations: Our language samples were collected from 37 children aged four to eight years. Even though they were followed at four time points, this is too small a sample and heterogeneity of language abilities is not observed along the lifespan in individuals with autism.Conclusions: Some but not all aspects of conversation skills grew over nine months in Chinese-speaking children with ASD. Parents’ verbal responsiveness was positively related to the children’s elicitation of appropriate responses.
Nexus Between Genome-Wide Copy Number Variations and Autism Spectrum Disorder in Northeast Han Chinese Population
Authors: Qiu Shuang , Cong Xianling , Li Yan , Shi Jikang , Qiu Yingjia , Guo Yanbo , Gu Yulu , Li Yong , Zhu Xiaojuan , Liu Yunkai , Qiao Yichun , Cheng Yi , Liu Yawen 
Published: January 2021

Abstract

Background: Autism spectrum disorder (ASD) is a common neurodevelopmental condition, with an increasing prevalence worldwide. Copy number variation (CNV), as one of genetic factors, is involved in ASD etiology. However, there exist substantial differences in terms of location and frequency of some CNVs in the general Asian population. Whole-genome studies of CNVs in Northeast Han Chinese samples are still lacking, necessitating our ongoing work to investigate the characteristics of CNVs in a Northeast Han Chinese population with clinically diagnosed ASD.Methods: We performed a genome-wide CNVs screening in Northeast Han Chinese individuals with ASD using array-based comparative genomic hybridization.Results: We found 22 kinds of CNVs (six deletions and 16 duplications) were potential pathogenic. These CNVs were distributed in chromosome 1p36.33, 1p36.31, 1q42.13, 2p23.1-p22.3, 5p15.33, 5p15.33-p15.2, 7p22.3, 7p22.3-p22.2, 7q22.1-q22.2, 10q23.2-q23.31, 10q26.2-q26.3, 11p15.5, 11q25, 12p12.1-p11.23, 14q11.2, 15q13.3, 16p13.3, 16q21, 22q13.31-q13.33, and Xq12-q13.1. Additionally, we found 20 potential pathogenic genes of ASD in our population, including eight protein coding genes (six duplications [DRD4, HRAS, OPHN1, SHANK3, SLC6A3, and TSC2] and two deletions [CHRNA7 and PTEN]) and 12 microRNAs genes (ten duplications [MIR202, MIR210, MIR3178, MIR339, MIR4516, MIR4717, MIR483, MIR675, MIR6821, and MIR940] and two deletions [MIR107 and MIR558]).Limitations: The sample size in our study may confer limited statistical power to discover significant findings. De novo or inherited of the CNVs were not be classified because of the lack of data from parents.Conclusions: We identified CNVs and genes implicated in ASD risks, conferring perception to further reveal ASD etiology.
Optimum of Dual Antiplatelet Duration and Followed Monotherapy in Diabetes Mellitus After Percutaneous Coronary Intervention With Drug-Eluting Stent Implantation: A Bayesian Network Meta-Analysis of 20536 Patients From 18 Randomized Trials
Authors: An Ke , Guo Peng , Qiu Shanhu , Zhu Wenwen , Cao Wuyou , Shi Jijing , Wang Shaohua 
Published: January 2021

Abstract
Background: To evaluate the efficacy and safety of short-term (≤3-month) dual antiplatelet therapy (DAPT), midterm (6-month) DAPT, standard-term (12-month) DAPT and extended-term (＞12-month) DAPT in diabetes after percutaneous coronary intervention (PCI) with drug-eluting stent (DES). To Compare discontinuation of DAPT followed by aspirin with P2Y12 inhibitor monotherapy for detailed optimal scheme.Methods: Randomized, controlled trials were searched using PubMed, Web of Science, Embase, Cochrane library and clinicaltrials.gov. up to October 10, 2020. A Bayesian network meta-analysis was conducted with a random-effect model.Results: A total of 18 randomized trials encompassing 20536 diabetic patients were included. Network analysis showed that short-term DAPT is best for reducing primary endpoint, which is superior to extended-term DAPT (odds ratio 0.48, 95% CI 0.25 to 0.85). Standard-term was also associated with reduce risk of primary endpoint in comparison with prolonged DAPT (0.56, 0.32 to 0.90). There was no noticeable difference with respect to primary endpoint between short-term DAPT followed by aspirin monotherapy and P2Y12 inhibitor monotherapy. No significant differences were observed in secondary endpoints, including all-cause mortality, cardiac mortality, MI, stroke, TVR, definite or probable stent thrombosis and major bleeding.Conclusions: Short-term DAPT was associated with the better primary endpoint benefit for patients with diabetes after PCI with DES, compared with extended-term DAPT. Although the optimal duration should balance risk-benefit ratio between personal ischemic and bleeding events, this study suggested short term DAPT followed by P2Y12 inhibitor monotherapy may be the optimal therapy for most diabetes after PCI with DES.
An association between inflammation and cerebral venous thrombosis: A retrospective study
Authors: Zhang Xifang , Ding Rui , Li Haiyan , Liu Yaqi , Ou weiyang , Hu Jiangang , Tian Yu , wang qiujing 
Published: January 2021

Abstract
Background
Evidence is currently accumulating for the role of inflammation in cerebral venous thrombosis (CVT). Neutrophil/lymphocyte ratio (NLR), platelet/lymphocyte ratio (PLR), monocyte/high-density lipoprotein ratio (MHR), and systematic immune-inflammation index (SII) are easily obtainable indicators of systemic inflammations. However, there were few studies on the relationship between them and CVT. Therefore, we aimed to evaluate the connection between the occurrence of CVT and the inflammatory markers described.
Methods
The samples from 150 participants (including 90 CVT and 60 controls) with similar baseline characteristics were collected in this retrospective study. The NLR, PLR, MHR, SII and file records were employed to compare CVT patients with the control group.
Results
The levels of NLR (3.93 [2.27, 7.87] vs. 1.65 [1.31, 2.06], P < 0.001), PLR (149.52 [98.39, 198.82] vs. 107.34 [83.31, 129.47], P < 0.001), SII (382.45 [273.51, 520.92] vs. 896.84 [559.89, 1591.87], P < 0.001) and MHR (0.51 [0.40, 0.64] vs. 0.41 [0.29, 0.53], P = 0.001) were significantly higher in the CVT group. After multivariate logistic regression analysis, the SII degree (13.136, [5.675, 30.407], P < 0.001) and MHR degree (2.620, [1.123, 6.113], P = 0.026) were found as independent predictors of CVT.
Conclusions
NLR, PLR, SII, and MHR may be able to predict the onset of CVT which confirmed that inflammation played an important role in CVT.
Postnatal Mental Health, Breastfeeding Beliefs, and Breastfeeding Practices in Rural China
Authors: Jiang Qi , Zhang Evelyn , Cohen Nourya , Ohtori Mika , Zhu Sabrina , Guo Yian , Johnstone Hannah Faith , Dill Sarah-Eve , Zhou Huan , Rozelle Scott D 
Published: January 2021

Abstract
Background

The importance of breastfeeding in low- and middle- income countries is well recognized, yet the importance of postnatal mental health on breastfeeding practices and beliefs in these settings has been understudied. This study investigates the associations between maternal mental health problems and breastfeeding beliefs as well as practices in rural China.
Methods

Cross-sectional data were collected from 742 mothers of infants under six months old in rural Sichuan Province, China. Surveys collected data on maternal mental health problems (depression, anxiety, and stress symptoms), breastfeeding beliefs (attitudes and self-efficacy), and breastfeeding practices. Ordinary least squares regression, multiple logistic regression and heterogeneous effect analyses were used to determine the associations between maternal mental health and breastfeeding outcomes.
Results

Among all respondents, 13% showed symptoms of depression, 16% anxiety, and 9% stress. The prevalence of full breastfeeding was 59.3%. Breastfeeding attitude was significantly associated with symptoms of depression (p = 0.023) and breastfeeding self-efficacy with symptoms of depression (p = 0.001) and symptoms of stress (p = 0.020). However, there were no significant associations between symptoms of mental health problems and full breastfeeding. The heterogeneous effects analyses revealed that full breastfeeding was negatively associated with stress symptoms when the infant was from a high-income family (p = 0.011). In addition, full breastfeeding was negatively associated with the father having a higher education level (p = 0.026, p = 0.048, and p = 0.020) and the infant being older than 2 months old (p = 0.000, p = 0.000, p = 0.00), regardless of maternal mental health problem symptoms.
Conclusion

Symptoms of maternal mental health problems are significantly associated with breastfeeding attitude and self-efficacy, yet has less of an association with breastfeeding practices. To improve breastfeeding practices, interventions need a multi-dimensional approach that should not only focus on improving maternal mental well-being but also consider demographic background characteristics.