bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effects of Different Molecular Weight Polysaccharides From Dendrobium officinale Kimura & Migo on Human Colorectal Cancer and Transcriptome Analysis of Differentially Expressed Genes
Authors: Tao Shengchang , Ren Zhiyao , Yang Zerui , Duan Shuna , Wan Zhongxian , Huang Jiahui , Liu Chenxing , Wei Gang 
Published: December 2021

We investigated the antitumor effects of four fractions of Dendrobium officinale Kimura &amp; Migo (D. officinale) polysaccharides with different molecular weights (Mw), Astragalus membranaceus polysaccharides (APS) and Lentinus edodes polysaccharides (LNT) on colorectal cancer (CRC) using a zebrafish xenograft model. Transcriptome sequencing was performed to further explore the possible antitumor mechanisms of D. officinale polysaccharides. Fractions of D. officinale polysaccharides, LNT, and APS could significantly inhibit the growth of HT-29 cells in a zebrafish xenograft model. One fraction of D. officinale polysaccharides called DOPW-1 (Mw of 389.98 kDa) exhibited the strongest tumor inhibition. Compared with the control group, RNA-seq revealed that the DOPW-1–treated experimental group had 119 differentially expressed genes (DEGs), of which 45 had upregulated expression and 74 had downregulated expression. Analyses using Gene Ontology and Kyoto Encyclopedia of Genes and Genomes suggested that the pathway “apoptosis-multiple species” was the most significantly enriched. Our data indicated that 1) fractions of D. officinale polysaccharides of Mw 389.98 kDa were most suitable against CRC; 2) DOPW-1 could be developed into a clinical agent against CRC; and 3) an apoptosis pathway is important for DOPW-1 to inhibit the proliferation of HT-29 cells.
Centella asiatica (L.) Urb. Prevents Hypertension and Protects the Heart in Chronic Nitric Oxide Deficiency Rat Model
Authors: Bunaim Mohd Khairulanwar , Kamisah Yusof , Mohd Mustazil Mohd Noor , Fadhlullah Zuhair Japar Sidik , Juliana Abdul Hamid , Muhammad Norliza 
Published: December 2021

Background: Hypertension is a major risk factor for cardiovascular disease (CVD), which is the number one cause of global mortality. The potential use of natural products to alleviate high blood pressure has been demonstrated to exert a cardioprotective effect. Centella asiatica (L.) Urb. belongs to the plant family Apiaceae (Umbelliferae). It contains a high amount of triterpenoid and flavonoid that have antioxidant properties and are involved in the renin-angiotensin-aldosterone system which is an important hormonal system for blood pressure regulation.Objective: This study aimed to investigate the effects of C. asiatica ethanolic extract on blood pressure and heart in a hypertensive rat model, which was induced using oral N(G)-nitro-l-arginine methyl ester (l-NAME).Methods: Male Sprague-Dawley rats were divided into five groups and were given different treatments for 8 weeks. Group 1 only received deionized water. Groups 2, 4, and 5 were given l-NAME (40 mg/kg, orally). Groups 4 and 5 concurrently received C. asiatica extract (500 mg/kg, orally) and captopril (5 mg/kg, orally), respectively. Group 3 only received C. asiatica extract (500 mg/kg body weight, orally). Systolic blood pressure (SBP) was measured at weeks 0, 4, and 8, while serum nitric oxide (NO) was measured at weeks 0 and 8. At necropsy, cardiac and aortic malondialdehyde (MDA) contents, cardiac angiotensin-converting enzyme (ACE) activity, and serum level of brain natriuretic peptide (BNP) were measured.Results: After 8 weeks, the administrations of C. asiatica extract and captopril showed significant (p &lt; 0.05) effects on preventing the elevation of SBP, reducing the serum nitric oxide level, as well as increasing the cardiac and aortic MDA content, cardiac ACE activity, and serum brain natriuretic peptide level.Conclusion:C. asiatica extract can prevent the development of hypertension and cardiac damage induced by l-NAME, and these effects were comparable to captopril.
Delineating the Relationships Between Motor, Cognitive-Executive and Psychiatric Symptoms in Female FMR1 Premutation Carriers
Authors: Hocking Darren R. , Loesch Danuta Z. , Stimpson Paige , Tassone Flora , Atkinson Anna , Storey Elsdon 
Published: December 2021

Introduction: Premutation expansions (55–200 CGG repeats) of the Fragile X Mental Retardation 1 (FMR1) gene on the X chromosome are associated with a range of clinical features. Apart from the most severe - Fragile X-Associated Tremor/Ataxia Syndrome (FXTAS) - where the most typical white matter changes affect cerebellar peduncles, more subtle changes may include impairment of executive functioning, affective disorders and/or subtle motor changes. Here we aimed to examine whether performance in selected components of executive functioning is associated with subclinical psychiatric symptoms in non-FXTAS, adult females carrying the FMR1 premutation.Methods and Sample: A total of 47 female premutation carriers (sub-symptomatic for FXTAS) of wide age range (26–77 years; M = 50.3; SD = 10.9) were assessed using standard neuropsychological tests, three motor rating scales and self-reported measures of psychiatric symptoms using the Symptom Checklist-90-Revised (SCL-90-R).Results: After adjusting for age and educational level where appropriate, both non-verbal reasoning and response inhibition as assessed on the Stroop task (i.e., the ability to resolve cognitive interference) were associated with a range of primary psychiatric symptom dimensions, and response inhibition uniquely predicted some primary symptoms and global psychiatric features. Importantly, lower scores (worse performance) in response inhibition were also strongly correlated with higher (worse) scores on standard motor rating scales for tremor-ataxia and for parkinsonism.Conclusion: These results provide evidence for the importance of response inhibition in the manifestation of psychiatric symptoms and subtle tremor-ataxia motor features, suggestive of the presence of early cerebellar changes in female premutation carriers.
Association of VEGF With Antianhedonic Effects of Repeated-Dose Intravenous Ketamine in Treatment-Refractory Depression
Authors: Zheng Wei , Gu Li-Mei , Zhou Yan-Ling , Wang Cheng-Yu , Lan Xiao-Feng , Zhang Bin , Shi Hai-Shan , Wang Dan-Feng , Ning Yu-Ping 
Published: December 2021

Objectives: To first explore the role of plasma vascular endothelial growth factor (VEGF) concentrations in ketamine's antianhedonic effects, focusing on Chinese patients with treatment-refractory depression (TRD).Methods: Seventy-eight patients with treatment-refractory major depressive disorder (MDD) or bipolar disorder (BD) were treated with six ketamine infusions (0.5 mg/kg). Levels of anhedonia were measured using the Montgomery–Åsberg Depression Rating Scale (MADRS) anhedonia item at baseline, day 13 and 26. Plasma VEGF concentrations were examined at the same time points as the MADRS.Results: Despite a significant reduction in anhedonia symptoms in individuals with treatment-refractory MDD (n = 59) or BD (n = 19) after they received repeated-dose ketamine infusions (p &lt; 0.05), no significant changes in plasma VEGF concentrations were found at day 13 when compared to baseline (p &gt; 0.05). The alteration of plasma VEGF concentrations did not differ between antianhedonic responders and non-responders at days 13 and 26 (all ps &gt; 0.05). Additionally, no significant correlations were observed between the antianhedonic response to ketamine and plasma VEGF concentrations (all ps &gt; 0.05).Conclusion: This preliminary study suggests that the antianhedonic effects of ketamine are not mediated by VEGF.
Developing Best Practice Guidance for Discharge Planning Using the RAND/UCLA Appropriateness Method
Authors: Tyler Natasha , Planner Claire , Byrne Matthew , Blakeman Thomas , Keers Richard N. , Wright Oliver , Pascall Jones Paul , Giles Sally , Keyworth Chris , Hodkinson Alexander , Taylor Christopher D. J. , Armitage Christopher J. , Campbell Stephen , Panagioti Maria 
Published: December 2021

Background: Discharge from acute mental health inpatient units is often a vulnerable period for patients. Multiple professionals and agencies are involved and processes and procedures are not standardized, often resulting in communication delays and co-ordination failures. Early and appropriate discharge planning and standardization of procedures could make inpatient care safer.Aim: To inform the development of a multi-component best practice guidance for discharge planning (including the 6 component SAFER patient flow bundle) to support safer patient transition from mental health hospitals to the community.Methods: Using the RAND/UCLA Appropriateness method, a panel of 10 professional stakeholders (psychiatrists, psychiatric nurses, clinical psychologists, pharmacists, academics, and policy makers) rated evidence-based statements. Six hundred and sixty-eight statements corresponding to 10 potential components of discharge planning best practice were rated on a 9-point integer scale for clarity, appropriateness and feasibility (median ≥ 7–9) using an online questionnaire then remote online face-to-face meetings.Results: Five of the six “SAFER” patient flow bundle components were appropriate and feasible for inpatient mental health. One component, “Early Flow,” was rated inappropriate as mental health settings require more flexibility. Overall, 285 statements were rated as appropriate and feasible. Forty-four statements were considered appropriate but not feasible to implement.Discussion: This consensus study has identified components of a best practice guidance/intervention for discharge planning for UK mental health settings. Although some components describe processes that already happen in everyday clinical interactions (i.e., review by a senior clinician), standardizing such processes could have important safety benefits alongside a tailored and timely approach to post-discharge care.
Perspectives of pharmacists in general practice from qualitative focus groups with patients during a pilot study
Authors: Mann Claire , Anderson Claire , Boyd Matthew , Karsan Yasmin , Emerson Tristan 
Published: December 2021

BackgroundUtilising skill mix in general practice is proposed as a solution to the demand–supply issue. Pharmacists can play an important role in this context, leading to an increase in training and funding for independent prescriber roles. A role for pharmacists in general practice was funded, piloted, and evaluated by NHS England (NHSE) from 2015.AimTo answer the following question: what is the patient perspective of pharmacists in patient-facing roles in general practice in the UK?Design & settingFocus group interviews exploring patient perspectives on the pharmacist role in the UK.MethodThirty-four patients were interviewed in five focus group (January–December 2016). Data were iteratively analysed using the one sheet of paper technique.ResultsWhile the public were aware of the primary care crisis, they were less well informed about potential solutions. Data showed patients primarily sought access to a clinician over expressing a preference for any type of clinician. Low awareness was shown about the pharmacist role and there was initial confusion about it. Acceptability levels were high. It was found that pharmacists added value and provided an expert medication-focused service, which had a positive impact on medicines use. Patients reported benefit from longer appointments, feeling they were not rushed, and that all their conditions were being considered holistically. They trusted pharmacists as experts in medication and trust was consolidated over time. Regular coaching from a pharmacist could lead to improved patient self-monitoring and self-care.ConclusionPharmacists can add value to the general practice team and this is recognised by patients.
Three-Dimensional Super-Resolved Imaging of Paraffin-Embedded Kidney Samples
Authors: Unnersjö-Jess David , Ramdedovic Amer , Höhne Martin , Butt Linus , Koehler Felix C. , Müller Roman-Ulrich , Hoyer Peter F. , Blom Hans , Schermer Bernhard , Benzing Thomas 
Published: December 2021

Key Points

Our previous protocols for 3D super-resolution kidney imaging have not been optimized to be compatible with paraffin-embedded samples.This study overcomes these limitations, allowing 3D super-resolved imaging in FFPE kidney blocks.This advancement opens up for 3D super-resolution kidney imaging of biobank material and in clinical settings.

Background
Diseases of the glomeruli, the renal filtration units, are a leading cause of progressive kidney disease. Assessment of the ultrastructure of podocytes at the glomerular filtration barrier is essential for diagnosing diverse disease entities, providing insight into the disease pathogenesis, and monitoring treatment responses.

Methods
Here we apply previously published sample preparation methods together with stimulated emission depletion and confocal microscopy for resolving nanoscale podocyte substructure. The protocols are modified and optimized in order to be applied to formalin-fixed paraffin-embedded (FFPE) samples.

Results
We successfully modified our protocols to allow for deep three-dimensional stimulated emission depletion and confocal imaging of FFPE kidney tissue with similar staining and image quality compared with our previous approaches. We further show that quantitative analysis can be applied to extract morphometrics from healthy and diseased samples from both mice and humans.

Conclusions
The results from this study could increase the feasibility of implementing optical kidney imaging protocols in clinical routines because FFPE is the gold-standard method for storage of patient samples.
PERANAN SISTEM PENGENDALIAN INTERNAL DALAM PENANGGULANGAN PIUTANG TAK TERTAGIH
Authors: Kuncara Valentina Christabella 
Published: December 2021

The internal control system, if implemented properly, will certainly create a systematic work procedure and make it in accordance with the rules used in the organization. This research aims to determine and also explain the role of the internal control system in handling uncollectible accounts receivable. This study uses a qualitative descriptive analysis method. This data is processed and obtained through observation, interviews and documentation. The population and sample in this study are social conditions with the sampling technique used is purposive sampling. The interviewees of this research are part of ARH and AR. The results of this study show that the role of the internal control system in overcoming uncollectible accounts receivable when the customer has become a WO is no longer paying the installments, but paying all the arrears. When the debtor is not with the unit, the company will involve a third party, namely PT which is engaged in securing assets.
La Cultura escrita como un recurso de poder.
Authors: Hernández Flores Gloria Elvira , Benítez Jaramillo José Federico 
Published: December 2021

El artículo aborda los procesos de apropiación y usos juveniles de la letra en el contexto escolar de educación secundaria y media superior. Pone énfasis en personas jóvenes y modalidades vulnerables, la juventud rural y urbana, así como de la telesecundaria y escuela media superior de organización social.
Su objetivo es exponer resultados de investigación de un cuerpo académico denominado Educación y poder. El proyecto se desarrolló entre 2018 y 2020 en el sur y norte del estado de México con perspectivas, comprensivo, interpretativa y horizontales, y enfoque sociocultural. Se trabajó a través de investigación tallerista que implicó participación y diálogo con los sujetos de la investigación.Sostiene que la cultura escrita es un medio de poder que permite la reflexión y análisis de jóvenes en el marco de las condiciones de vulnerabilidad educativa y los posiciona ante su contexto y ante sí mismos.
Pengaruh Pemberian Madu Terhadap Kadar Hemoglobin Ibu Hamil Trimester III di BPM Ny “T” Kecamatan Purwadadi Kabupaten Subang
Authors: Rianti Ririn , Choirunissa Risza , Rukmaini Rukmaini 
Published: December 2021

Dalam kehamilan, anemia bisa berpengaruh buruk terutama saat kehamilan, persalinan dan nifas. Ibu hamil yang menderita anemia memiliki kemungkinan akan mengalami perdarahan post partum. Berdasarkan hasil data di BPM “T” tahun 2020 terdapat Ibu hamil trimester III yang mengalami anemia yaitu sebanyak 30 orang (27,6%). Tujuan dari penelitian ini yaitu untuk melihat pengaruh pemberian madu terhadap kadar hemoglobin ibu hamil trimester III di BPM “T” kecamatan purwadadi kabupaten subang. Metodologi penelitian ini menggunakkan quasi eksperiment group pretest-posttest desaign dengan membagi satu kelompok. Populasi sebanyak 92 orang ibu hamil dan Sampel dalam penelitian ini berjumlah 30 orang ibu hamil yang mengalami anemia. Teknik pemilihian sampel dengan menggunakkan total sampling. Instrumen penelitian terdiri dari dokumentasi dan lembar inform concent. Data dianalisis menngunakkan Dependen t test untuk mengetahui ada pengaruh atau tidak. Hasil penelitian menunjukkan bahwa terdapat ada pengaruh pemberian madu terhadap kadar hemoglobin dengan hasil signifikasi pada pretest mendapatkan hasil p-value (Sig) 0,009 dan posttest didapatkan hasil p-value (Sig) 0,550 (p 0.05). Simpulan dan saran yang dapat diberikan yaitu bahwa pemberian madu efektif dalam meningkatkan kadar Hb ibu hamil, maka diharapkan setelah penelitian ini berakhir pihak BPM akan mensosialisasikan terhadap para ibu hamil khususnya yang menderita anemia untuk rutin mengkonsumsi madu untuk meningkatkan kadar ibu hamil.Kata Kunci : Pemberian Madu dan Kadar HB Ibu Hamil Trimester III.
Hubungan Pengetahuan dan Sikap Perilaku Pencegahan Transmisi Covid-19 di Kelurahan Halim Perdana Kusuma
Authors: Chadaryanti Diyah , Muhafilah Ilah 
Published: December 2021

Dunia sedang menghadapi wabah Pandemi yang di sebabkan virus jenis baru ditetapkan oleh WHO yakni Severe Acute Respiratory Syndrome Coronavirus – 2 (SARS-CoV – 2) sebutan penyakit Corona Virus Disease 2019 (COVID-19). CDC ( Center for Disease Control and Prevention) menyatakan, pasien yang terindikasi COVID-19 menunjukkan gejala ISPA, Ringan sampai berat.Sudah banyak warga dunia dilaporkan dan terkonfirmasi positif Coronavirus COVID19 termasuk di Indonesia. Pemerintah sudah konsen terhadap pencegahan penyebaran COVID-19 meluas, seperti menggunakan masker jika terpaksa keluar rumah, rajin mencuci tangan dan menjaga jarak. Tujuan penelitian adalah untuk menganalisis hubungan antara pengetahuan dan sikap dengan perilaku pencegahan transmisi Covid-19, warga RT 02/05 Kelurahan Halim Perdanakusuma. Populasi penelitian adalah warga RT 02/05 Kelurahan Halim Perdanakusuma. Sementara sampel dalam penelitian ini berjumlah 51 orang. Hasil penelitian menunjukan bahwa variabel sikap berhubungan dengan perilaku pencegahan Transmisi Covid-19, dengan nilai Pv. 0,039 dan OR sebesar .4,190. Sementara variabel pengetahuan diperoleh hasil tidak berhubungan dengan perilaku pencegahan Transmisi Covid-19 dengan nilai P sebesar 0,40. Perlu adanya kesadaran setiap orang untuk berperilaku mencegah transmisi covid-19. Edukasi terkait bahaya penularan covid-19 harus tetap dilakukan secara masif, Secara tidak lansung memunculkan sikap positif setiap orang untuk berperilaku mencegah penularan covid-19.Kata Kunci : Perilaku Pencegahan, Tranmisi COVID -19 ,Pengetahun dan Sikap
PENGEMBANGAN SISTEM INFORMASI MANAJEMEN PELAYANAN PELAPORAN SAPU BERSIH PUNGUTAN LIAR PADA KEMENKOPOLHUKAM JAKARTA
Authors: Ependi Ependi , Muharom Fadhlan 
Published: December 2021

Seiring banyaknya kasus pemungutan liar yang terjadi di masyarakat, maka diperlukan peningkatan pelayanan yang dilakukan oleh Satuan Tugas Sapu Bersih Pungutan Liar pada Kemenkopolhukam, khususnya di wilayah Jakarta. Untuk meningkatkan pelayanan kepada masyarakat, maka diperlukan sistem pengolahan informasi data laporan pungutan liar. Sejauh ini sistem pengelolaan laporan yang digunakan masih secara manual, yakni masyarakat atau pelapor datang ke kantor Satgas Saber Pungli, lalu petugas yang akan mengolah data laporan tersebut. Perancangan Sistem Informasi Manajemen Aplikasi Pelayanan Pelaporan dikembangkan dengan model-model pengembangan sistem seperti Undified Modelling Language (UML), Entity Relationship Diagram (ERD), Normalisasi hingga Desain Input dan Output. Dengan suatu rancangan Sistem Informasi Manajemen pelayanan pelaporan berbasis komputer yang tepat, akurat dan relevan, dapat menyelesaikan masalah yang berhubungan dengan proses pelaporan sapu bersih pungutan liar. Setelah keseluruhan sistem selesai dibuat, dapat diketahui bahwa dengan rancangan sistem informasi Manajemen Aplikasi Pelayanan Pelaporan yang baru dapat menghasilkan informasi yang cepat, akurat dan relevan.
PENGEMBANGAN APLIKASIKONVERSI MATA KULIAH MENGGUNAKAN FRAMEWORKCODEIGNITER DAN BOOTSTRAP (Studi Kasus : Sekolah Tinggi Ilmu Komunikasi Profesi Indonesia)
Authors: Sopian Abu 
Published: December 2021

Students who enroll in the Sekolah Tinggi Ilmu Komunikasi Profesi Indonesia are not only from High School Graduates or from the same level. But students who enroll are also available from Bachelor Degree (D3) or called Level Transfer. For that, it is necessary to do equalization or conversion of courses from Bachelor Degree (D3). Currently the conversion of subjects in still done manually. Forthat, it is necessary to develop an application that can help in converting for subject. In this study the development of course conversion applications is used Software Development Life Sycle (SDLC) method with the waterfall model. Based on the analysis of the system design above, it can be concluded that the Model View Controller (MVC) architecture can be applied to the codeigniter framework and MVCarchitecturemaking it easier to repair changes to the source code without changing the entire logic and fungtion properly and meet the objectives the beginning of the development of this subject conversion application.
A Survey of the Hadamard Maximal Determinant Problem
Authors: Browne Patrick , Egan Ronan , Hegarty Fintan , Ó Catháin Padraig 
Published: December 2021

In a celebrated paper of 1893, Hadamard established the maximal determinant theorem, which establishes an upper bound on the determinant of a matrix with complex entries of norm at most 1. His paper concludes with the suggestion that mathematicians study the maximum value of the determinant of an $n \times n$ matrix with entries in $\{ \pm 1\}$. This is the Hadamard maximal determinant problem.
This survey provides complete proofs of the major results obtained thus far. We focus equally on upper bounds for the determinant (achieved largely via the study of the Gram matrices), and constructive lower bounds (achieved largely via quadratic residues in finite fields and concepts from design theory). To provide an impression of the historical development of the subject, we have attempted to modernise many of the original proofs, while maintaining the underlying ideas. Thus some of the proofs have the flavour of determinant theory, and some appear in print in English for the first time.
We survey constructions of matrices in order $n \equiv 3 \mod 4$, giving asymptotic analysis which has not previously appeared in the literature. We prove that there exists an infinite family of matrices achieving at least 0.48 of the maximal determinant bound. Previously the best known constant for a result of this type was 0.34.