bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mapping differences in mammalian distributions and diversity using environmental DNA from rivers
Authors: Broadhurst Holly A. , Gregory Luke M. , Bleakley Emma K. , Perkins Joseph C. , Lavin Jenna V. , Bolton Polly , Browett Samuel S. , Howe Claire V. , Singleton Natalie , Tansley Darren , Sales Naiara Guimarães , McDevitt Allan D. 
Published: April 2021

AbstractAimFinding more efficient ways to monitor, and estimate the diversity of, mammalian communities is a major step towards their management and conservation. Environmental DNA (eDNA) from river water has recently been shown to be a viable method for biomonitoring mammalian communities. Yet, most of the studies to date have focused on the potential for eDNA to detect individual species, with little focus on describing patterns of community diversity and structure. In this study, we focus on the sampling effort required to reliably map the diversity and distribution of semi-aquatic and terrestrial mammals and allow inferences of community structure surrounding rivers.LocationSoutheastern EnglandMethodsWe used eDNA metabarcoding on water samples collected along two rivers and a beaver enclosure over two days, targeting terrestrial and semi-aquatic mammals. Mammalian community diversity and composition was assessed based on species richness and β-diversity. Differences between river communities were calculated and partitioned into nestedness and turnover, and the sampling effort required to rapidly detect semi-aquatic and terrestrial species was evaluated based on species accumulation curves and occupancy modelling.ResultseDNA metabarcoding efficiently detected 25 wild mammal species from five orders in two days of sampling, representing the vast majority (82%) of the species expected in the area. The required sampling effort varied between orders, with common species (generally rodents, deer and lagomorph species) more readily detected, with carnivores detected less frequently. Measures of species richness differed between rivers (both overall and within each mammalian order) and patterns of β-diversity revealed the importance of species replacement in sites within each river, against a pattern of species loss between the two rivers.Main conclusionseDNA metabarcoding demonstrated its capability to rapidly detect mammal species, allowing inferences of community composition that will better inform future sampling strategies for this Class. Importantly, this study highlights the potential use of eDNA data for investigating mammalian community dynamics over different spatial scales.
tdLanYFP, a yellow, bright, photostable and pH insensitive fluorescent protein for live cell imaging and FRET-based sensing strategies
Authors: Bousmah Yasmina , Valenta Hana , Bertolin Giulia , Singh Utkarsh , Nicolas Valérie , Pasquier Hélène , Tramier Marc , Merola Fabienne , Erard Marie 
Published: April 2021

AbstractYellow fluorescent proteins (YFP) are widely used as optical reporters in Förster Resonance Energy Transfer (FRET) based biosensors. Although great improvements have been done, the sensitivity of the biosensors is still limited by the low photostability and the poor fluorescence performances of YFPs at acidic pHs. Here, we characterize the yellow fluorescent protein, tdLanYFP, derived from the tetrameric protein from the cephalochordateB. lanceolatum, LanYFP. With a quantum yield of 0.92 and an extinction coefficient of 133 000 mol−1.L.cm−1, it is, to our knowledge, the brightest dimeric fluorescent protein available. Contrasting with EYFP and its derivatives, tdLanYFP has a very high photostabilityin vitroand in live cells. As a consequence, tdLanYFP allows imaging of cellular structures with sub-diffraction resolution using STED nanoscopy and is compatible with the use of spectro-microscopies in single molecule regimes. Its very low pK1/2of 3.9 makes tdLanYFP an excellent tag even at acidic pHs. Finally, we show that tdLanYFP is valuable FRET partner either as donor or acceptor in different biosensing modalities. Altogether, these assets make tdLanYFP a very attractive yellow fluorescent protein for long-term or single-molecule live cell imaging including FRET experiments at acidic pH.
Long read sequencing reveals novel isoforms and insights into splicing regulation during cell state changes
Authors: Wright David J , Hall Nicola , Irish Naomi , Man Angela L , Glynn Will , Mould Arne , De Los Angeles Alejandro , Angiolini Emily , Swarbreck David , Gharbi Karim , Tunbridge Elizabeth M , Haerty Wilfried 
Published: April 2021

ABSTRACTAlternative splicing (AS) is a key mechanism underlying cellular differentiation and a driver of complexity in mammalian neuronal tissues. However, understanding of which isoforms are differentially used or expressed and how this affects cellular differentiation remains unclear. Long read sequencing allows full-length transcript recovery and quantification, enabling transcript-level analysis of AS processes and how these change with cell state. Here, we utilise Oxford Nanopore Technologies sequencing to produce a custom annotation of a well-studied human neuroblastoma cell line and to characterise isoform expression and usage across differentiation. We identify many previously unannotated features, including a novel transcript of the voltage-gated calcium channel subunit gene,CACNA2D2. We show differential expression and usage of transcripts during differentiation, and identify a putative molecular regulator underlying this state change. Our work highlights the potential of long read sequencing to uncover previously unknown transcript diversity and mechanisms influencing alternative splicing.
Sustained coevolution of phage Lambda andEscherichia coliinvolves inner as well as outer membrane defenses and counter-defenses
Authors: Burmeister Alita R. , Sullivan Rachel M. , Gallie Jenna , Lenski Richard E. 
Published: April 2021

AbstractBacteria often evolve resistance to phage through the loss or modification of cell-surface receptors. InEscherichia coliand phage λ, such resistance can catalyze a coevolutionary arms race focused on host and phage structures that interact at the outer membrane. Here, we analyze another facet of this arms race involving interactions at the inner membrane, wherebyE. colievolves mutations in mannose permease-encoding genesmanYandmanZthat impair λ’s ability to eject its DNA into the cytoplasm. We show that thesemanmutants arose concurrently with the arms race at the outer membrane. We tested the hypothesis that λ evolved an additional counter-defense that allowed them to infect bacteria with deletedmangenes. The deletions severely impaired the ancestral λ, but some evolved phage grew well on the deletion mutants, indicating they regained infectivity by evolving the ability to infect hosts independently of the mannose permease. This coevolutionary arms race fulfills the model of an inverse-gene-for-gene infection network. Taken together, the interactions at both the outer and inner membranes reveal that coevolutionary arms races can be richer and more complex than is often appreciated.IMPACT STATEMENTLaboratory studies of coevolution help us understand how host defenses and pathogen counter-defenses change over time, which is often essential for predicting the future dynamics of host-pathogen interactions. One particular model, termed “inverse-gene-for-gene” coevolution, predicts that coevolution proceeds through alternating steps, whereby hosts lose the features exploited by pathogens, and pathogens evolve to exploit alternative features. Using a classic model system in molecular biology, we describe the nature and timing of a previously overlooked step in the coevolution ofE. coliand bacteriophage lambda. Our work demonstrates that this mode of coevolution can profoundly re-shape the interactions between bacteria and phage.
Robust and annotation-free analysis of alternative splicing across diverse cell types in mice
Authors: Benegas Gonzalo , Fischer Jonathan , Song Yun S. 
Published: April 2021

AbstractAlthough alternative splicing is a fundamental and pervasive aspect of gene expression in higher eukaryotes, it is often omitted from single-cell studies due to quantification challenges inherent to commonly used short-read sequencing technologies. Here, we undertake the analysis of alternative splicing across numerous diverse murine cell types from two large-scale single-cell datasets—theTabula Murisand BRAIN Initiative Cell Census Network—while accounting for understudied technical artifacts and unannotated isoforms. We find strong and general cell-type-specific alternative splicing, complementary to total gene expression but of similar discriminatory value, and identify a large volume of novel isoforms. We specifically highlight splicing variation across different cell types in primary motor cortex neurons, bone marrow B cells, and various epithelial cells; and show that the implicated transcripts include many genes which do not display total expression differences. To elucidate the regulation of alternative splicing, we build a custom predictive model based on splicing factor activity, recovering several known interactions while generating new hypotheses, including potential regulatory roles for novel alternative splicing events in critical genes includingKhdrbs3andRbfox1. We make our results available using public interactive browsers to spur further exploration by the community.
Why narcissistic individuals engage in unethical pro-organizational behavior. Testing a moderated mediation model
Authors: Toirova Munisa , Baek Yoonjung 
Published: April 2021

PurposeThe purpose of the current research is to study the relationship between narcissism and unethical pro-organizational behavior (UPB), and also examine whether status striving mediates the relationship between narcissism and UPB among individuals with high organizational identification.Design/methodology/approachData that was used to test the research model were collected from five companies in the trading sector in Tashkent, Uzbekistan. The final sample included 200 responses, among all participants 29.5% were in a leadership position.FindingsThe current research found that narcissism leads individuals to exhibit a willingness to engage in UPB. Furthermore, status striving mediates the relationship between narcissism and UPB among individuals with high organizational identification.Research limitations/implicationsResearch did not use actual reported UPB but measured employees' willingness to engage in UPB.Practical implicationsOrganizations should develop special ethics guidance to change the employee's perceptions of UPB from the act of helping or protecting the organization to undesirable behavior in the organization. Moreover, the organization may develop an ethical counseling program, by which individuals may perceive that ethical behavior is valuable for the organization.Originality/valueCurrent study examines the relationship between narcissism and UPB. Moreover, it provides empirical support for the notion that the relationship between narcissism and UPB is mediated by status striving among individuals with high organizational identification.
Exploring the influence of supply chain collaboration on supply chain visibility, stakeholder trust, environmental and financial performances: a partial least square approach
Authors: Baah Charles , Acquah Innocent Senyo Kwasi , Ofori Daniel 
Published: April 2021

PurposeThe need to stay competitive amidst ever-changing business environment has shifted competitive strategies from firms to supply chains. Managers are now basing competitive strategies on supply chains acknowledging that supply chains present competitive advantages among other resources. The purpose of the study is to explore the predictive relevance of supply chain collaboration and the extent to which it influences supply chain visibility, stakeholder trust, environmental and financial performances. This study focused on manufacturing firms due to their supplier relationships, consumption of resources, energy and emissions of greenhouse gasses.Design/methodology/approachThe study adopted a survey research design, a quantitative approach and partial least square structural equation modelling technique in making data analysis and interpretations due to its suitability for predictive research models as is the case in this study.FindingsThe study hypothesized that supply chain collaboration positively and significantly interacts with supply chain visibility, stakeholder trust, environmental and financial performances. The study results confirmed supply chain collaboration as a significant, positive and a robust influence on supply chain visibility, stakeholder trust, environmental and financial performances thereby projecting win-win scenarios for firms that engage in collaborative supply chain practices.Originality/valueThe study is among the few to indicate findings in relation to the scope of supply chain collaboration's potency in influencing performance from the perspective of manufacturing firms operational in an emerging economy. Thus, this study contributes to understanding the wider scope of supply chain collaboration, its interactions with other firm variables and how it informs decisions of managers, scholars and supply chain partners.
Integrated thinking, earnings manipulation and value creation: Malaysian empirical evidence
Authors: Mohammed Nor Farizal , Sutainim Nor Aqilah , Islam Md. Shafiqul , Mohamed Norhayati 
Published: April 2021

PurposePrior literature proposes that integrated reporting (IR) drives integrated thinking (IT), enabling an organisation to create value for stakeholders in both quantitative (economic performance) and qualitative manners (beyond financially-oriented information). Fraud triangle theory also predicts that earnings manipulation may also affect the creation of value. Thus, this study seeks to provide empirical evidence on the relationship between IT, earnings manipulation and value creation.Design/methodology/approachThis data sample comprises of 497 observations from 2014 to 2018 of the top 100 market capitalisation of Malaysian public listed companies (PLCs) in Bursa Malaysia. This study used an index score for IT variable and Beneish’s M-score as a proxy to detect earnings manipulations and to classify the companies into non-manipulators and manipulator companies. Value creation measurements consist of four variables under shareholder's value creation and one variable represents value creation through innovation.FindingsThe findings show that IT is significantly related to value creation, whereas earnings manipulation had no significant relationship with value creation except for value creation measured using Tobin's Q ratio. The alarming finding is that a fraud predictor, namely earning manipulation, measured by Beneish-M, is not a predictor of whether companies are creating better or less value.Originality/valueThis study is among the early literature that provides empirical evidence of the relationship between IT and value creation. Furthermore, this paper adds to look at the association of earning manipulation and value creation.
Strategy on development of innovative ecosystem in customer values
Authors: Wang Xinxin 
Published: April 2021

PurposeIn a fierce competition environment in which product prices fall sharply or even tend to be free, only by realizing differentiated customer value through continuous innovation can companies respond quickly and effectively to changes and enhance their core competitiveness.Design/methodology/approachThe innovation ecosystem takes the enterprise as the core, based on stakeholders such as partners, customers and intermediaries, and is highly concentrated in an industry or a region to form close horizontal and vertical links with each other[4]. With the goal of continuously creating differentiated customer value, the internal resources of the collaborative system are integrated, and through the rapid flow of knowledge, material and value between the various entities, the overall value-added ability is continuously improved.FindingsThe innovation ecosystem is the core for users to create expected and dream experience. It combines different knowledge systems of groups and individuals with different characteristics and backgrounds to create an environment where various ideas can effectively collide and recombine and integrate resources through multiple channels to form an organism.Originality/valueEcological factors in the production and application of technology diffusion and knowledge in the process of mutual influence, in the process of dynamic interactive learning and self-reinforcing, identify and understand the customer value, coordinated development of the overall operation of product and service system, to form a consistent brand and improve customer loyalty and ultimately improve the innovation ecosystem value.
A novel methodology for optimum energy consumption in wireless sensor networks
Authors: Abbas Mohamed , Otayf Nasser 
Published: April 2021

PurposeThe purpose of this paper is to minimize energy usage by maximizing network life in the creation of applications and protocolsDesign/methodology/approachThis paper presents a novel methodology for optimum energy consumption in wireless sensor networks. The proposed methodology introduces some protocols and logarithms that effectively contributed to reducing energy consumption in these types of networks.FindingsThe results of that comparison showed the ability of those logarithms and protocols to reduce that energy but in varying proportions. It can be concluded that a significant reduction in energy consumption approximately 50% could be obtained by the proposed methodology.Originality/valueHere, a novel methodology for optimum energy consumption in wireless sensor networks has been introduced.
Exchange rate appreciation, trade competition and technology transaction: evidence from China
Authors: Cheng Dingping , Gan Sumei 
Published: April 2021

PurposeThe purpose of this study is mainly to investigate the stimulating effect on technology transactions of trade competition resultant from RMB appreciation.Design/methodology/approachThis study uses Chinese provincial panel data from 1998 to 2015 and utilizes GMM method to estimate the stimulating effect of RMB appreciation on technical transactions through trade competition.FindingsThe results demonstrate that RMB appreciation can encourage enterprises to make use of domestic technology market resources for innovation. Specifically, the increase in imports due to the appreciation of RMB can generate technology spill-over and significantly promote technology trade. The export competition resultant from RMB appreciation can also encourage domestic and foreign enterprises to enhance export competitiveness through increased technology transactions.Originality/valueThe current research investigates the impact of exchange rate on independent innovation, but this study demonstrates the influence of exchange rate on technology transactions. In addition, the data in this study cover 1998–2015 in China and thus contributes to determining the effects of exchange rate appreciation in emerging countries.
Psychological safety and individual performance: the mediating effect of intrapreneurial behavior
Authors: Mahmoud Mahmoud Ahmad , Ahmad Shuhymee , Poespowidjojo Donny Abdul Latief 
Published: April 2021

PurposeThe objective of this paper is to extend the extant literature on the relationship between psychological safety (PS) and individual performance (IP) through the mediating influence of intrapreneurial behavior (IB). Therefore, the social exchange theory (SET) and psychological entrepreneurship theory (PET) are integrated to achieve this objective.Design/methodology/approachSurvey method of data collection was engaged through self-administered questionnaire. The sample of 355 medium enterprises (MEs) production/operations managers was analyzed by means of partial least square (PLS) structural equation modeling (SEM).FindingsThe result shows that PS has a significant direct relationship with both IP and IB; likewise, IB has a significant positive influence on IP. The mediating influence of IB on the PS–IP relationship was also confirmed.Practical implicationsThe result suggests that PS–IP relationship will be better explained when IB is fortified among managers. Therefore, MEs could stimulate production/operations manager performance by encouraging IB through PS–IP relationship.Originality/valueTo the authors’ knowledge, this paper is the first to study the influence of individual-level PS on the complete individual-level IB and the mediating influence of individual IB on PS–IP relationship. Moreover, the intraprenerurship literature is relatively lacking expressly among developing countries and precisely Nigeria. As such, the attention of researchers is important on the role of individual-level IB in MEs against the assumption that entrepreneurial events are limited to firm-level concerns.
Dynamic capabilities: antecedents and implications for firms' performance
Authors: Correia Ricardo Jorge , Teixeira Mário Sérgio , Dias José G. 
Published: April 2021

PurposeThis paper aims to explore a new causal link between learning, market and entrepreneurial orientations and firms' performance by introducing dynamic capabilities and competitive advantages as mediator variables.Design/methodology/approachThe mediating role of dynamic capabilities and competitive advantages is tested using a sample of 1,190 Portuguese firms, and structural equation models.FindingsIt is shown that dynamic capabilities mediate the relationship between the three orientations–learning, market and entrepreneurial–and competitive advantages of differentiation and cost leadership, and both competitive advantages lead to firm's performance. It is also shown that learning orientation is an antecedent of market orientation and entrepreneurial orientation.Practical implicationsThis research shows that firm's performance depends on the capacity of firms to learn, innovate, be proactive, take risks and collect the best market data. Indeed, by optimizing the internal management and knowledge dissemination, firms will develop a set of capabilities and competitive advantages that lead to an appropriate response to market challenges.Originality/valueThis study tests the relationship between strategic orientations and firm's performance by taking the mediating effects of dynamic capabilities and competitive advantages into account. This research was conducted in Portugal.
Remittances, electricity consumption and electric power losses in Jamaica
Authors: Das Anupam , McFarlane Adian 
Published: April 2021

PurposeThe purpose of this paper is to examine the impact of remittance inflows (remittances) on electricity consumption and electric power losses in Jamaica.Design/methodology/approachThe authors use annual data from 1976 to 2014 and apply vector error correction modelling, Granger causality testing and impulse response analysis.FindingsFirst, the authors find that there is co-integration between remittances and the energy variables, namely electricity consumption and electric power losses. Second, short-run Granger causality exists between the energy variables and remittances. This causality is bidirectional between the energy variables and positive changes in remittances, but it is unidirectional running from the energy variables to negative movements in remittances. Third, the authors find that in the long-run remittances have a negative relationship with electric power losses and a positive relationship with the consumption of electricity.Practical implicationsFindings from this paper will help to elucidate the relationship between electricity consumption, and electric power losses, and remittances.Social implicationsThe problem of electric power losses is acute in Jamaica and it is mostly due to theft. At the same time, Jamaica receives significant remittances. Social policy could have a role to encourage the use of remittances to help stem the theft of electricity.Originality/valueThis is the first study that examines the relationships between remittances, electricity consumption and electric power losses.