bluuebunny/crossref_metadata_2025_split huggingface dataset:
Inequalities in Smoking and Quitting-Related Outcomes Among Adults With and Without Children in the Household 2013–2019: A Population Survey in England
Authors: Kock Loren , Brown Jamie , Shahab Lion , Tattan-Birch Harry , Moore Graham , Cox Sharon 
Published: October 2021

Abstract

Introduction
Smoking among those who live with children is an important influence on smoking initiation among children. This study assessed socioeconomic inequalities in smoking and quitting-related outcomes among all adults with and without children in the household.

Aims and Methods
Monthly repeat cross-sectional household survey of adults (16+) from 2013–2019 in England (N = 138 583). We assessed the association between cigarette smoking and quitting-related outcomes and having children in the household, and whether these relationships were moderated by occupational social grade (categories AB–E from most to least advantaged). Trends in smoking prevalence among adults with and without children in the household were explored.

Results
In adjusted analysis, the association of having children in the household with smoking prevalence depended on social grade: smoking prevalence was between 0.71 (95% confidence interval 0.66–0.77) and 0.93 (0.88–0.98) times lower among social grades AB–D with children in the household relative to those without. Conversely, it was 1.11 (1.05–1.16) times higher among social grade E. Yearly prevalence declined similarly among those with and without children (both prevalence ratio: 0.98, 95% confidence interval 0.97–0.99). Motivation to stop smoking was higher among those with children than those without, but lower among disadvantaged than more advantaged groups. Social grades D–E had greater heavy smoking, but higher prevalence of past-month quit attempts.

Conclusions
Among the most disadvantaged social grade in England, smoking prevalence was higher in those with children in the household than without. To attenuate future smoking-related inequalities, there is an urgent need to target support and address barriers to quitting and promote longer-term quit success.

Implications
In the most disadvantaged occupational social grade, having children in the household was associated with higher smoking prevalence compared with not having children. This contrasts with all other social grades in which there was lower comparative smoking prevalence among those with than without children in the household. Without attention this disparity could exacerbate existing and future health inequalities related to smoking.
Out of the East (or North or South): A Response to Philip Slavin
Authors: Green Monica H 
Published: October 2021

Abstract
This article responds to Philip Slavin’s ‘Out of the West: Formation of a Permanent Plague Reservoir in South-Central Germany (1349–1356) and Its Implications’. Genetics has transformed the study of plague, one of the most lethal diseases in human history. But this technically demanding science raises questions of what constitutes valid evidence and supportable argument when examining historical phenomena at a microscopic level. Slavin argues that two new lineages of Yersinia pestis, the causative organism of plague, were seeded in central Germany following the Black Death; appearing sequentially, one lineage caused plague outbreaks in the 1350s and early 1360s, only to retreat and be replaced by a second lineage. Here, evidence is adduced to support the early central European proliferation of one lineage of Y. pestis, but also to suggest that the second lineage arose simultaneously in a different locale, outside Europe and within different epidemiological parameters. Because of the inherent rarity of biological evidence, the reconstruction of epidemiological phenomena will always require consilience with archaeological and documentary sources. Establishing ‘best practices’ of analysis and verification in this emerging multidisciplinary field has implications not only for Europe’s four hundred-year experience with plague, but for all fields of global health history.
High-Light-Induced Stress Activates Lipid Deacylation at the Sn-2 Position in the Cyanobacterium Synechocystis Sp. PCC 6803
Authors: Kojima Kouji , Matsumoto Ui , Keta Sumie , Nakahigashi Kenji , Ikeda Kazutaka , Takatani Nobuyuki , Omata Tatsuo , Aichi Makiko 
Published: October 2021

Abstract
Cyanobacterial mutants defective in acyl-acyl carrier protein synthetase (Aas) produce free fatty acids (FFAs) because the FFAs generated by deacylation of membrane lipids cannot be recycled. An engineered Aas-deficient mutant of Synechocystis sp. PCC 6803 grew normally under low-light (LL) conditions (50 µmol photons m−2 s−1) but was unable to sustain growth under high-light (HL) conditions (400 µmol photons m−2 s−1), revealing a crucial role of Aas in survival under the HL conditions. Several-times larger amounts of FFAs were produced by HL-exposed cultures than LL-grown cultures. Palmitic acid accounted for ∼85% of total FFAs in HL-exposed cultures, while C18 fatty acids (FAs) constituted ∼80% of the FFAs in LL-grown cultures. Since C16 FAs are esterified to the sn-2 position of lipids in the Synechocystis species, it was deduced that HL irradiation activated deacylation of lipids at the sn-2 position. Heterologous expression of FarB, the FFA exporter protein of Neisseria lactamica, prevented intracellular FFA accumulation and rescued the growth defect of the mutant under HL, indicating that intracellular FFA was the cause of growth inhibition. FarB expression also decreased the ‘per-cell’ yield of FFA under HL by 90% and decreased the proportion of palmitic acid to ∼15% of total FFA. These results indicated that the HL-induced lipid deacylation is triggered not by strong light per se but by HL-induced damage to the cells. It was deduced that there is a positive feedback loop between HL-induced damage and lipid deacylation, which is lethal unless FFA accumulation is prevented by Aas.
Maintaining the Chloroplast Redox Balance through the PGR5-Dependent Pathway and the Trx System Is Required for Light-Dependent Activation of Photosynthetic Reactions
Authors: Okegawa Yuki , Tsuda Natsuki , Sakamoto Wataru , Motohashi Ken 
Published: October 2021

Abstract
Light-dependent activation of chloroplast enzymes is required for the rapid induction of photosynthesis after a shift from dark to light. The thioredoxin (Trx) system plays a central role in this process. In chloroplasts, the Trx system consists of two pathways: the ferredoxin (Fd)/Trx pathway and the nicotinamide adenine dinucleotide phosphate (NADPH)–Trx reductase C (NTRC) pathway. In Arabidopsis (Arabidopsis thaliana) mutants defective in either pathway, the photoreduction of thiol enzymes was impaired, resulting in decreased carbon fixation. The close relationship between the Fd/Trx pathway and proton gradient regulation 5 (PGR5)-dependent photosystem I cyclic electron transport (PSI CET) in the induction of photosynthesis was recently elucidated. However, how the PGR5-dependent pathway is involved in the NTRC pathway is unclear, although NTRC has been suggested to physically interact with PGR5. In this study, we analyzed Arabidopsis mutants lacking either the PGR5 or the chloroplast NADH dehydrogenase-like complex (NDH)-dependent PSI CET pathway in the ntrc mutant background. The ntrc pgr5 double mutant suppressed both the growth defects and the high non-photochemical quenching phenotype of the ntrc mutant when grown under long-day conditions. By contrast, the inactivation of NDH activity with the chlororespiratory reduction 2-2 mutant failed to suppress either phenotype. We discovered that the phenotypic rescue of ntrc by pgr5 is caused by the partial restoration of Trx-dependent reduction of thiol enzymes. These results suggest that electron partitioning to the PGR5-dependent pathway and the Trx system needs to be properly regulated for the activation of the Calvin–Benson–Bassham cycle enzymes during the induction of photosynthesis.
What do the Rural Folks Think? Perceptions of Police Performance
Authors: Ruddell Rick , O’Connor Christopher 
Published: October 2021

Abstract
Several highly publicized incidents have drawn the public’s attention to the problem of rural crime in Canada, and this focus is appropriate given that rates of rural crime in most provinces are higher than in urban areas. This study reports the results of an examination of urban and rural residents’ perceptions of the police in Saskatchewan, Canada. Controlling for their socio-demographic characteristics, prior victimization, perceptions of crime, and contact with the police, the results of 1,791 phone surveys reveal that rural residents are less likely than their urban counterparts to indicate their police do a good job of enforcing the laws, promptly responding to calls for service, providing them with crime prevention information, ensuring their safety, or cooperating with them to address their concerns. We also found that both urban and rural residents who felt their communities were unsafe or neutral (neither safe nor unsafe) were less likely to indicate the police did a good job in all the seven categories of police performance examined in this research. Implications for rural policing practice and research are identified considering these findings.
Rational engineering of an erythropoietin fusion protein to treat hypoxia
Authors: Lee Jungmin , Vernet Andyna , Gruber Nathalie G , Kready Kasia M , Burrill Devin R , Way Jeffrey C , Silver Pamela A 
Published: October 2021

Abstract
Erythropoietin enhances oxygen delivery and reduces hypoxia-induced cell death, but its pro-thrombotic activity is problematic for use of erythropoietin in treating hypoxia. We constructed a fusion protein that stimulates red blood cell production and neuroprotection without triggering platelet production, a marker for thrombosis. The protein consists of an anti-glycophorin A nanobody and an erythropoietin mutant (L108A). The mutation reduces activation of erythropoietin receptor homodimers that induce erythropoiesis and thrombosis, but maintains the tissue-protective signaling. The binding of the nanobody element to glycophorin A rescues homodimeric erythropoietin receptor activation on red blood cell precursors. In a cell proliferation assay, the fusion protein is active at 10−14 M, allowing an estimate of the number of receptor–ligand complexes needed for signaling. This fusion protein stimulates erythroid cell proliferation in vitro and in mice, and shows neuroprotective activity in vitro. Our erythropoietin fusion protein presents a novel molecule for treating hypoxia.
ASSESSMENT OF EXTERNAL OCCUPATIONAL DOSE OF PHOSPHATE MINE WORKERS IN SAUDI ARABIA USING THERMOLUMINESCENT DOSEMETERS
Authors: Shubayr N , Alashban Y , Al-Shehri S , Almurayshid M 
Published: October 2021

Abstract
Workers in the mining industry face the problem of radiation exposure from naturally occurring radioactive materials. Thus, this study aimed to estimate a Hp (10) doses for mine workers in the phosphate mining industry in Saudi Arabia. The personal dose equivalent [Hp (10)] of 606 mine workers in the phosphate mining industry in Saudi Arabia were obtained from 2016 to 2019 using thermoluminescent dosemeters (TLDs). The results showed that the annual mean Hp (10) of all mine workers averaged over the study period was 0.66 mSv (SD = 0.45), which is within the range of occupational doses reported worldwide. Most of the Hp (10) were &lt;0.49 mSv, and the maximum Hp (10) was 3.55 mSv. Nonetheless, internal exposure should be evaluated along with external exposure to estimate a comprehensive baseline occupational dose for mine workers in the phosphate mining industry in Saudi Arabia.
Channeling Good Images but Not Substantive Blessings? Education and Social Contact on Pro-Migrant Sentiments in an Internal Migration Setting
Authors: Tong Yuying , Nie Tianzhu , Piotrowski Martin 
Published: October 2021

AbstractEducation and intergroup contact are two of the most influential channels for reducing host society members’ hostility toward immigrants, but recent studies suggest that these two channels are only able to shift their impressions. Yet, a shift in impression does not necessarily translate into endorsement of integration policies, resulting in a “principle-policy paradox” in multiracial societies. Little is known about whether this paradox holds in circumstances where racial boundaries are less salient and rigid. In this study, we examine whether such a paradox exists in urban China, where racial/ethnic hostility is negligible but the Hukou system has created institutional segregation between local residents and migrants. Using data collected from twelve middle-size cities and one metropolis, we examine urban residents’ attitudes toward rural migrants from multiple domains. We find that education is positively associated with locals’ positive judgments about rural migrants, with concern about social justice playing a mediating role. However, there is a lack of association between education and the endorsement of integration policy or social distance. Compared with education, social contact, in particular kinship contact, is associated with support in more substantive domains, ranging from integration policies to a closer social distance. Nevertheless, social contact does not lead to the support for the most pivotal policy change of granting Hukou. The findings indicate that both education and social contact are limited in engendering the extension of benefits to migrants across contexts, but intergroup contact is more capable of doing so in a society with a more fluid group boundary.
Spatial and temporal coevolution of N2 neuraminidase and H1 and H3 hemagglutinin genes of influenza A virus in US swine
Authors: Zeller Michael A , Chang Jennifer , Vincent Amy L , Gauger Phillip C , Anderson Tavis K 
Published: October 2021

Abstract
The neuraminidase (NA) and hemagglutinin (HA) are essential surface glycoproteins of influenza A virus (IAV). In this study, the evolution of subtype N2 NA paired with H1 and H3 subtype HA in swine was evaluated to understand if the genetic diversity of HA and NA were linked. Using time-scaled Bayesian phylodynamic analyses, the relationships of paired swine N2 with H1 or H3 from 2009 to 2018 were evaluated. These data demonstrated increased relative genetic diversity within the major N2 clades circulating in swine in the USA (N2.1998 between 2014 and 2017 and N2.2002 between 2010 and 2016). Preferential pairing was observed among specific NA and HA genetic clades. Gene reassortment between cocirculating influenza A strains resulted in novel pairings that persisted. The changes in genetic diversity in the NA gene were quantified using Bayesian phylodynamic analyses, and increases in diversity were observed subsequent to novel NA–HA reassortment events. The rate of evolution among NA–N2 clades and HA–H1 and HA–H3 clades were similar. Bayesian phylodynamic analyses demonstrated strong spatial patterns in N2 genetic diversity, but frequent interstate movement of rare N2 clades provided opportunity for reassortment and emergence of new N2–HA pairings. The frequent regional movement of pigs and their influenza viruses is an explanation for the documented patterns of reassortment and subsequent changes in gene diversity. The reassortment and evolution of NA and linked HA evolution may result in antigenic drift of both major surface glycoproteins, reducing vaccine efficacy, with subsequent impact on animal health.
Association of Preoperative Depression and Anxiety With Long-term Opioid Use After Hysterectomy for Benign Indications
Authors: Carey Erin T. , Moore Kristin J. , Young Jessica C. , Bhattacharya Manami , Schiff Lauren D. , Louie Michelle Y. , Park Jihye , Strassle Paula D. 
Published: October 2021

OBJECTIVE:
To assess whether preoperative depression or anxiety is associated with increased risk of long-term, postoperative opioid use after hysterectomy among women who are opioid-naïve.

METHODS:
We conducted an observational cohort study of 289,233 opioid-naïve adult women (18 years or older) undergoing hysterectomy for benign indications from 2010 to 2017 using IBM MarketScan databases. Opioid use and refills in the 180 days after surgery and preoperative depression and anxiety were assessed. Secondary outcomes included 30-day incidence of emergency department visits, readmission, and 180-day incidence of opioid complications. The association of depression and anxiety were compared using inverse-probability of treatment weighted log-binomial and proportional Cox regression.

RESULTS:
Twenty-one percent of women had preoperative depression or anxiety, and 82% of the entire cohort had a perioperative opioid fill (16% before surgery, 66% after surgery). Although perioperative opioid fills were relatively similar across the two groups (risk ratio [RR] 1.07, 95% CI 1.06–1.07), women with depression or anxiety were significantly more likely to have a postoperative opioid fill at every studied time period (RRs 1.44–1.50). Differences were greater when restricted to persistent use (RRs 1.49–2.61). Although opioid complications were rare, women with depression were substantially more likely to be diagnosed with opioid dependence (hazard ratio [HR] 5.54, 95% CI 4.12–7.44), and opioid use disorder (HR 4.20, 95% CI 1.97–8.96).

CONCLUSION:
Perioperative opioid fills are common after hysterectomy. Women with preoperative anxiety and depression are more likely to experience persistent use and opioid-related complications.
Complications of Morbid Obesity in the Treatment of Acetabular Fractures: Re-evaluating Outcomes a Decade Later
Authors: Robertson Michael J. , Lee Rowdy C. , Bergin Patrick F. , Graves Matthew L. , Russell George V. 
Published: October 2021

Objectives:
To determine the rate of perioperative complications between morbidly obese (body mass index greater than 40 kg/m2) and nonmorbidly obese patients undergoing operative treatment of acetabular fractures across 2 periods (2000–2005 and 2012–2019).

Design:
Retrospective, case–control study.

Setting:
Level I academic trauma center.

Patients:
Four hundred thirty-five consecutive patients from 2000 to 2005 and 216 consecutive patients from 2012 to 2019 with acetabular fractures treated by a single surgeon.

Intervention:
Operative fixation of acetabular fracture.

Main Outcome Measurements:
Outcome variables include positioning time, operative time, estimated blood loss, hospital stay, wound complications, and perioperative complications.

Results:
Twenty-eight morbidly obese and 188 nonmorbidly obese patients from 2012 to 2019, as well as 41 morbidly obese patients and 394 nonmorbidly obese patients from 2000 to 2005 were included in the study. The relative risk (RR) of wound complications between 2012 and 2019 groups was significantly higher for morbidly obese patients (RR = 5.31, P = 0.009) but has decreased significantly for morbidly obese patients between 2000–2005 and 2012–2019 (RR = 0.31, P = 0.017). The rate of total perioperative complications was similar between morbidly obese and nonmorbidly obese groups from 2012 to 2019 (21% vs. 8%, P = 0.230). For morbidly obese patients, the rate of total perioperative complications decreased significantly between 2000–2005 and 2012–2019 (63% vs. 21% P = 0.010).

Conclusion:
Acetabular fracture surgery can be safely performed in morbidly obese patients. Although obesity remains a significant risk factor for wound complications, the risk for morbidly obese patients has decreased significantly since our initial investigation because of adaptations to surgical techniques and surgeon's experience.

Level of Evidence:
Prognostic Level III. See Instructions for Authors for a complete description of levels of evidence.
Are Serum Ion Levels Elevated in Pediatric Patients With Metal Implants?
Authors: Mathew Smitha E. , Xie Yong , Bagheri Leila , Claton Liam E. , Chu Lin , Badreldin Amr , Abdel Matthew P. , van Wijnen Andre J. , Haft Geoffrey F. , Milbrandt Todd A. , Larson A. Noelle 
Published: October 2021

Background:
Previous studies report elevated serum titanium (Ti) levels in children with spinal implants. To provide additional data on this topic, we sought to assess serum ion levels at multiple timepoints in pediatric patients with growing spine devices, spinal fusion instrumentation, and extremity implants placed for fracture treatment. We hypothesized that serum Ti, cobalt (Co), and chromium (Cr) levels would be elevated in pediatric patients with growing spine devices compared with patients with extremity implants.

Methods:
Pediatric patients undergoing any primary spine implant placement, those with spine implant revision or removal surgery and patients with other appendicular implant removal had serum Ti, Co, and Cr ion levels drawn at the time of surgery. Fifty-one patients (12 growing spine devices, 13 fusions, and 26 extremity implants) had one set of labs, 31 of whom had labs drawn both preoperatively and postoperatively. Biopsies obtained from tissue specimens at the time of implant revision were analyzed histologically for the presence of metal debris and macrophage activity.

Results:
Patients with growing spine implants had elevated serum Ti (3.3 vs. 1.9 ng/mL, P=0.01) and Cr levels (1.2 vs. 0.27 ng/mL, P=0.01) in comparison to patients with fusion rods or extremity implants. With respect to patients with extremity implants, patients with growing spine devices had elevated serum Ti (3.3 vs. 0.98 ng/mL, P=0.013), Co (0.63 vs. 0.26 ng/mL, P=0.017), and Cr levels (1.18 vs. 0.26 ng/mL, P=0.005). On matched pairs analysis, patients who had labs drawn before and after spine implantation had significant increase in serum Ti levels (0.57 vs. 3.3 ng/mL, P=0.02). Histology of tissue biopsies adjacent to growing spine implants showed presence of metal debris and increased macrophage activity compared with patients with extremity implants.

Conclusion:
Serum Ti, Co, and Cr levels are elevated in children with spinal implants compared with those with extremity implants, particularly in those with growing spine devices. However, the clinical significance of these findings remains to be determined.

Level of Evidence:
Level II—prospective comparative study.