bluuebunny/crossref_metadata_2025_split huggingface dataset:
Monitoring Cortico-cortical Evoked Potentials using Only Two 6-strand Strip Electrodes for Gliomas Extending to the Dominant Side of Frontal Operculum During One-step Tumor Removal Surgery
Authors: Saito Taiichi , Muragaki Yoshihiro , Tamura Manabu , Maruyama Takashi , Nitta Masayuki , Tsuzuki Shunsuke , Fukui Atsushi , Koriyama Shunichi , Kawamata Takakazu 
Published: September 2021

Abstract
Resection of the dominant side of gliomas extending to the frontal operculum has a high-risk of permanent language dysfunction. Here, we report recording cortico-cortical evoked potentials (CCEP) using only two 6-strand strip electrodes to monitor language-related fibers intraoperatively. We examined whether this simple procedure is useful for removing gliomas extending to the dominant side of frontal operculum. This study included five cases of glioma extending to the left frontal operculum. The frontal language area (FLA) was first identified by functional mapping during awake craniotomy. Next, a 6-strand strip electrode was placed just above the FLA, while on the temporal lobe side, an electrode was placed so as to slide parallel to the Sylvian fissure toward the posterior language area. Electrical stimulation was performed using the electrode on the frontal side, and CCEPs were measured from the electrode on the temporal side. CCEPs were detected in all cases. Immediately after surgery, all patients demonstrated language dysfunction to varying degrees. CCEP decreased up to 10% in one patient, who recovered language function after 24 months. CCEP decreased slightly up to 80% in one, and in the three other cases, CCEPs did not change. These four patients soon recovered language function within 2 weeks to 1 month. This study confirmed the utility of CCEP monitoring using only two 6-strand strip electrodes during one-step surgery. We believe this simple method helped in monitoring intraoperative language function and aided predicting its postoperative recovery, in patients with gliomas extending to the dominant side of frontal operculum.
Biodegradable Blends of Thermoplastic Waxy Starch and Poly(ε-caprolactone) Obtained by High Shear Extrusion: Rheological, Mechanical, Morphological and Thermal Properties
Authors: Manoel Anderson F. , Claro Pedro , Mattoso Luiz H. C. , Marconcini Jose M. , Mantovani Gerson L. 
Published: September 2021

Abstract
The aim of this work was to develop polymer blends of plasticized thermoplastic waxy starch (TPS) and poly(ɛ-caprolactone) (PCL) using the largest amount of TPS possible to obtain a biodegradable material motivated by sustainability issues and to replace petrochemical-based polymers with alternatives based on biopolymers. Addition of TPS to other polymers has been used to obtain cheaper and increasingly biodegradable final products. However, TPS presents limited mechanical properties, and mixing with other polymers such as PCL is necessary to overcome these limitations and improve its processability. TPS was processed by extrusion and thermo-compression using 30 wt% glycerol. The blends were suitably processed by extrusion and further injected. The TPS/PCL blends were studied by varying the amount of PCL in a range of 10 wt% in increasing order of addition. Thermal analysis showed that introducing PCL in TPS increased Tonset because of the higher thermal stability of the former, and that the obtained blends presented a behavior intermediate to that of neat polymers.
Deep Learning Analysis to Automatically Detect the Presence of Penetration or Aspiration in Videofluoroscopic Swallowing Study
Authors: Kim Jeoung Kun , Choo Yoo Jin , Choi Gyu Sang , Shin Hyunkwang , Chang Min Cheol , Park Donghwi 
Published: September 2021

Abstract
Background: Videofluoroscopic swallowing study (VFSS) is currently considered the gold standard to precisely diagnose and quantitatively investigate dysphagia. However, VFSS interpretation is complex and requires consideration of several factors. Purpose: Therefore, considering the expected impact on dysphagia management, this study aimed to apply deep learning to detect the presence of penetration or aspiration in VFSS of patients with dysphagia automatically.Materials and Methods: The VFSS data of 190 participants with dysphagia were collected. A total of 10 frame images from one swallowing process were selected (five high-peak images and five low-peak images) for the application of deep learning in a VFSS video of a patient with dysphagia. We applied a convolutional neural network (CNN) for deep learning using the Python programming language. For the classification of VFSS findings (normal swallowing, penetration, and aspiration), the classification was determined in both high-peak and low-peak images. Thereafter, the two classifications determined through high-peak and low-peak images were integrated into a final classification.Results: The area under the curve (AUC) for the validation dataset of the VFSS image for the CNN model was 0.946 for normal findings, 0.885 for penetration, and 1.000 for aspiration. The average AUC was 0.962.Conclusion: This study demonstrated that deep learning algorithms, particularly the CNN, could be applied for detecting the presence of penetration and aspiration in VFSS of patients with dysphagia.
In Vivo Evaluation of Nanostructured Lipid Carrier Systems (NLCs) in Mice Bearing Prostate Cancer Tumours
Authors: Akanda Mushfiq , Getti Giulia , Douroumis Dennis 
Published: September 2021

Abstract
Nanostructure lipid carriers (NLCs) were developed for the delivery of curmumin (CRN), a potent anticancer agent with low bioavailability, for the treatment of prostate cancer. NLCs prepared using High Pressure Homogenization (HPH) with around 150 nm particle size, -40V ζ-potential and excellent long-term stability. Cellular uptake of CRN-SLN, showed nanoparticle localization in the cytoplasm around the nucleus. CRN-NLCs were assessed using flow cytometry and found to cause early and late apoptotic events at 100 μg/ml CRN concentrations. CRN-NLC nanoparticles were administrated to nude mice with LNCaP prostate cancer xenografts and demonstrated substantial tumour volume suppression (40%) with no weight loss compared to pure CRN (ethanolic solution). Overall, NLCs were proved a suitable carrier for passive drug delivery and cancer treatment.
Spatial and Texture Analysis of Root System Architecture with Earth Mover's Distance (STARSEED)
Authors: Peeples Joshua , Xu Weihuang , Gloaguen Romain , Rowland Diane , Zare Alina , Brym Zachary 
Published: September 2021

Abstract
Root system architectures are complex, multidimensional, and challenging to characterize effectively for agronomic and ecological discovery. We propose a new method, Spatial and Texture Analysis of Root System architEcture with Earth mover's Distance (STARSEED), for comparing root architectures that incorporate spatial information through a novel application of the Earth Mover's Distance (EMD).We illustrate that the approach captures the response of sesame root systems for different genotypes and soil moisture levels. STARSEED provides quantitative and visual insights into changes that occur in root architectures across experimental treatments.STARSEED can be easily generalized to other plants and provides insight into root system architecture development and response to varying growth conditions not captured by existing root architecture metrics and models. The code and data for our experiments are publicly available: https://github.com/GatorSense/STARSEED.
A Case Study of the Registration of Essential Medicines in the East African Community
Authors: Green Alexander Karl , Brhlikova Petra , Ocan Moses , Pollock Allyson 
Published: September 2021

Abstract
Objective: To quantify the essential medicine status of registered medicines in the East African Community (EAC) for antimicrobial medicines and medicines for non-communicable diseases.Methods: The National Drug Registers (NDRs) of Kenya, Tanzania, and Uganda (February 2018) were compared with their respective national Essential Medicine Lists (EMLs) to determine the registration status of essential medicines as an indicator of their availability. The number of registered products was analysed for essential medicines and selected non-essential medicines. Findings: Many essential medicines in the EAC are not registered: Kenya 28% (175/632), Tanzania 50% (400/797), Uganda 40% (266/663). Approximately, only one-third of products on the NDRs are essential medicines: Kenya (29%), Tanzania (36%), and Uganda (42%). 23% to 42% of registered essential medicines only have 1 or 2 products. 42 medicines accounted for over 4100 medical products, half of which were non-essential products. Conclusions: This novel analysis provides insight into how registration of medicines is likely to a be a major barrier to accessing essential medicines. The under registration of essential medicines and the over registration of non-essential medicines demonstrates a mismatch between the market of medicines being produced and registered and the health needs of the East African Community. Joint regional medicine authorisation programs such as the EAC Medicines Registration Harmonization Project have potential to improve access to key medicines if essential medicines are prioritised.
When to Cryopreserve Ovarian Tissue: Effects of Chemotherapy on the Ovarian Reserve by Studying Follicular Density, Tissue Damage and Follicular Apoptosis
Authors: Labrune Elsa , Bianchetti Serge , Lepinasse Odile , Soignon Gaelle , Salle Bruno , Lornage Jacqueline 
Published: September 2021

Abstract
BackgroundPatients scheduled to receive chemotherapy should be counseled on fertility preservation. Known gonadotoxic chemotherapies such as alkylating agents have a high risk of altering ovarian reserve. In some cases, the urgency of treatment requires the use of chemotherapy before fertility preservation, which will be carried out at a later stage. Most often the ovarian tissue is cryopreserved. The aim of our study is to investigate the impact of chemotherapies on follicular density, tissue damage and apoptosis of reserve follicles.ResultsWe included 140 patients: 63 patients, mean age 18.8 years, were included in the group no chemotherapy (group A) and 77 patients, mean age 17.1 years, in the group presence of chemotherapy before ovarian conservation (group B). None of the patients had had pelvic radiotherapy prior to ovarian cryopreservation. The histological parameters studied were: follicular density, the presence of cortical fibrosis and the presence of vessel abnormalities. We selected 12 patients from group A and 15 patients from group B, comparable in age and pathology, for whom we evaluated follicle apoptosis by immunostaining cleaved caspase 3. We demonstrated an inverse relationship between follicular density and age (p<0.0001), as well as a lack of effect of chemotherapy on follicular density (p=0.87). There was no difference in other histological parameters. On the other hand, we showed an impact of chemotherapies, especially alkylating agents, on the apoptosis of ovarian follicles (p<0.0001).ConclusionOur study is the largest cohort reported to date. This work underlines that conservation of ovarian tissue after chemotherapy remains possible.
Plasma exosomal miRNA-205 can be used for early diagnosis and prognostic evaluation of ovarian cancer
Authors: zhu zehua , Zhu Zehua , Chen Zhaojun , Wang Mingxin , Zhang Min , Chen Yiwen , Yang Xiao , Zhou Changjun , Liu Yuhua , Hong Liquan , Zhang Lahong 
Published: September 2021

Abstract
Background

Ovarian cancer (OC) is a serious threat to women's health around the world and new biomarkers are urgently needed for early detection. Our previous published research have confirmed that miR-205 can promote the invasion and metastasis of OC cells by inhibiting the expression of the tumor suppressor gene TCF21. This study is based on the previous work, using exosomal liquid biopsy technology to detect the expression levels of the four genes miR-205, CA125, HE4 and TCF21 in the plasma exosomes of OC patients; at the same time, combined with the clinicopathological parameters of OC patients data analysis aims to provide efficient and non-invasive laboratory testing basis for the early diagnosis of OC.
Methods

Collected 36 OC patients diagnosed in local hospitals from September 2020 to July 2021 as OC group, selected 31 cases of surgically diagnosed ovarian benign lesions for benign group and 32 healthy people who underwent physical examination during the same period as a control group, and used transmission electron microscope (TEM), western blotting (WB) and nanoparticle tracking analysis (NTA) to identify after extracting plasma exosomes from kits. The expression levels of miR-205, CA125, HE4 and TCF21 genes in plasma exosomes were detected by real-time fluorescence quantitative PCR (qRT-PCR) method. At the same time, combined with the relationship between clinical pathological parameters in OC patients, the Receiver Operating Characteristic (ROC) curves were used to evaluate the diagnostic efficacy of plasma exosomal miR-205, CA125, HE4 and TCF21 for OC.
Results

Plasma exosomes were successfully isolated; the expression level of plasma exosomal miR-205 in the OC group was higher than that of the benign group and the control group, and the difference was statistically significant (P < 0.05); plasma exosomal miR-205 was elevated during the III-IV period of OC and lymph node metastasis; combined detection of plasma exosomal miR-205 can improve the diagnostic ability of OC.
Conclusion

The plasma exosomal miR-205 can be used as a potential tumor biomarker to improve the diagnostic effectiveness of OC.
Material Flow Analysis and Resource Recovery Potential Analysis of Selected Fruit, Vegetable and Nut Waste in Kenya
Authors: De Meester Steven , Dulo Benson , Githaiga John , Raes Katleen 
Published: September 2021

Abstract
In Kenya, agriculture is an important economic activity, which implies that a significant amount of bio-waste is generated. This is on one hand a waste management problem, but on the other hand, it is an opportunity for creating a sustainable bioeconomy. Therefore, this study investigates the potential recovery of bioresources from Kenyan bio-waste. The study first quantifies occurrence, current usage and disposal of three selected biomass types, being banana, potato and coconut waste. Next, material flow analysis (MFA) is used to systematically track the mass flow of these wastes. Finally, the potential of biomolecules, biomaterials and bioenergy from the waste streams is evaluated. The study revealed that 6007, 426 and 49.5 kt of banana, potato and coconut biomass is wasted. All these wastes can be biorefined, offering potential towards recovery of; flavonoids (88.3 kt), starch (377 kt), cellulose (2000.7 kt) and biogas (1757.0 GWh), being the total potential of the main bioresources from the three waste streams. The study therefore, concluded that, with proper waste collection, sorting and valorisation, there is a huge potential for bioeconomy in Kenya, at the same time reducing waste management problems.
Weather Patterns Driving Atmospheric Rivers, Santa Ana Winds, Floods, and Wildfires During California Winters Provide Evidence for Increasing Fire Risk
Authors: Guirguis Kristen , Gershunov Alexander , Hatchett Benjamin , Shulgina Tamara , DeFlorio Michael J. , Subramanian Aneesh C. , Guzman-Morales Janin , Aguilera Rosana , Clemesha Rachel , Corringham Thomas W. , Monache Luca Delle , Reynolds David , Tardy Alex , Small Ivory , Ralph Fred Martin 
Published: September 2021

Abstract
Floods caused by atmospheric rivers and wildfires fanned by Santa Ana winds are common occurrences in California with devastating societal impacts. Planning for these types of events is critical to protect life and property, and extending the lead-times of predictability for these types of events improves emergency response. A better understanding of linkages between large-scale atmospheric circulation patterns and extreme weather represents an important step towards improving predictability and preparedness on subseasonal-to-seasonal (S2S) timescales and for climate change adaptation. In this work, we show that winter weather variability in California, including the occurrence of extreme and impactful events, is linked to four atmospheric circulation regimes over the North Pacific Ocean previously named and identified as the “NP4 modes”. These modes interact on daily timescales to produce recurring winter weather patterns that are major drivers of atmospheric river landfalls, Santa Ana winds, floods, and wildfires. Many recent California natural hazard events resulted from compounding and cascading extremes including frequent atmospheric river landfalls (wet, fuel-producing years) or lack thereof (drought) and followed by Sana Ana-driven wildfires that render the landscape susceptible to hydrologic hazards posed by short-duration high-intensity precipitation events. This historical perspective of atmospheric circulation and impacts over 70 years reveals that weather patterns are changing in a way that enhances wildfire risk in California, while the frequency of weather patterns linked to historical floods is not diminishing. These changes highlight the rising hazards of compounding weather extremes in California’s present and future.
Does the Masticatory Function Interfere With the Nutritional Status and Quality of Life in Individuals After Treatment for Head and Neck Cancer?
Authors: Froes Renata Camilla Favarin , Carvalho² Mariana Inri , Rodrigues³ Raquel Rosa , Silva Paulo Sérgio dos Santos , Berretin-Felix Giédre 
Published: September 2021

Abstract
Head and neck cancer damages the oral health and chewing performance, impairing the nutritional status and quality of life even after treatment completion. Objective: This study investigated the effect of treatment on the chewing function, the influence on oral health in chewing function and the relationships between chewing, nutritional status and quality of life in individuals after treatment of head and neck cancer. Methods: The study was conducted on twenty individuals who were evaluated by surface electromyography; nutritional status; evaluation of oral health and quality of life. Results: The results of electromyography showed an asymmetric pattern, with predominance of activity on the working side. The correlations evidenced that the greater the use of upper dentures and caries activity, the greater the asymmetry of muscle activity, while the greater need of lower denture led to more chewing cycles. The lower the body fat percentage, the greater the symmetry of muscle activity; and the lower the recruitment of temporalis muscles, the greater the physical limitation. Conclusions: It was concluded that, in individuals after treatment of head and neck cancer, there was relationship between the correlated variables.
The Immunoregulatory Effect of Mechanical Stress on Three-dimensional Cultured Mesenchymal Stem Cells After Extensive Expansion
Authors: Du Fang-Ying , Zhao Na , Bao Lei , Lei Jing , Liu An-Qi , Gao Ying-Feng , Bao Li-Hui , Ni Hua , Zhou Feng , Yu Xiao-Rui , Hu Cheng-Hu 
Published: September 2021

Abstract
Background: Mesenchymal stem cells (MSCs) have been used to treat immunopathy, and three-dimensional (3D) cultured MSCs show enhanced immunomodulatory property compared with those in two-dimensional (2D) culture. However, both the regulatory mechanisms remain unclear. The aim of the study was to investigate the role of mechanical stress in maintaining the immunomodulatory function of 2D and 3D cultured MSCs.Methods: Umbilical cord mesenchymal stem cells (UC-MSCs) were plated on tissue culture plastic (TCP) as 2D culture and 3D cultured UC-MSCs were seeded in matrigel. Surface markers, clonogenicity, proliferation and immunoregulatory property of UC-MSCs were evaluated. Meanwhile, we established the mouse models of colitis and type 1 diabetes mellitus (T1DM) to reveal the pharmacotherapeutic effects of 3D cultured MSCs in vivo. The effect of changing mechanical stress by modulating Yes-associated protein (YAP) on immunomodulatory function of 2D and 3D cultured UC-MSCs was evaluated by immunofluorescent analysis, real-time quantitative polymerase chain reaction (qPCR) and western blot.Results: We verified early passage UC-MSCs in 2D and 3D cultures exhibited stemness, immunomodulatory property and therapeutic efficacy against immunopathy. However, these characteristics of 2D cultured UC-MSCs were impaired after extensive expansion, whereas 3D culture extended them for several passages by activating YAP. Moreover, prostaglandin E2 (PGE2) could up-regulate YAP to improve the immunomodulatory ability of 2D cultured UC-MSCs after extensive expansion. Conclusions: This work found for the first time that the significance of mechanical stress in maintaining immunoregulatory function of 2D and 3D cultured UC-MSCs, providing a new idea for improving the efficacy of MSCs-based immunotherapy.
The Influence of Family Structure and Adult Attachment Style on Theft Behaviors in Men: a Case-Control Study
Authors: Wang Xu , Xu Jiajun 
Published: September 2021

Abstract
Background: Theft has leaded to significant costs to commerce and legal system, but its psychological mechanisms and risk factors are barely explored. This study aims to investigate the influence of family structure and adult attachment style on theft behaviors in men. Methods: 257 men with a conviction for theft (theft group) and 405 male college students (control group) were interviewed with Experiences in Close Relationships Scale and a general questionnaire about socio-demographic information, family structure and criminal records. Results: Compared with control group, long-term residency, family economic situation, number of siblings, father’s presence before 5 years old, parents’ state of existence and parents’ marital status of theft group were significantly different (P<0.05). Living in urban area, from relatively poor family, not living with father before 5 years old and parents not divorced increased the likelihood of theft in men (P<0.05). While father not alive and having no siblings decreased the likelihood of theft in men (P<0.05). Then theft group was further divided into first offender group and recidivist group. First offender group had a significantly higher proportion of living with mother/father before 5 years old and father alive than recidivist group (P<0.05). Not living with mother before 5 years old and father not alive increased the possibility of multiple thefts, while preoccupied attachment decreased such possibility (P<0.05). Conclusions: This study suggested that risk factors for theft and multiple thefts were different. A variety of targeted strategies related to family structure and attachment styles are needed to prevent theft from occurring and reoccurring.