bluuebunny/crossref_metadata_2025_split huggingface dataset:
Diversify your workflow! - An inconvenient suggestion to analyze spike data from intracranial recordings
Authors: Okkesim Şükrü , Rastogi Shavika , Christ Olaf , Hubka Peter , Rosskothen-Kuhl Nicole , Hofmann Ulrich G. 
Published: March 2021

AbstractAn important challenge of neuroscience research and future brain machine interfacing is the reliable assignment of spikes to putative neurons. By means of extracellular recordings, researchers try to match different types action potentials with their putative neuronal source and timing. Unfortunately, this procedure is by far not standardized and reliable, leading to many different suggestions and as many differing results. It appears that sharing of data is thus hampered by different processing pipelines in different labs, thus playing along the reproducibility crisis in neurosciences. To systematically shed light on this issue, we present preliminary results of several easy event detection schemes on one data set, meant to illustrate the inconsistencies arising from different processing pipelines already in its initial step. The results indicate that thresholding choices alter findings due to a lack of a ground truth for spike sorting. We suggest to increase reliability in findings by only accepting and further processing events accepted by more than one processing pipeline.
Low-Cost, Modular Modification to a Desktop 3D Printer for General Purpose Gel/Paste Extrusion & Direct Ink Writing
Authors: Leech D. J. , Lightfoot S. , Huson D. , Stratakos A. 
Published: March 2021

AbstractWe propose a design for a simple paste extruder modification that can be used for the selective deposition and patterning of gels and pastes, using a desktop 3D printer as the primary platform. This technology has found use with a variety of materials in seemingly disparate fields, including the printing of ceramics, food and biological materials, each with a variety of material-specific solutions to enhance printability. However, we focus on a syringe-pump driven system that is simple, low-cost, modular, easily assembled and highly modifiable with a low barrier of entry in order to maximise the generalisability and range of printable materials.
Corticosterone differentially modulates time-dependent fear generalization following mild or moderate fear conditioning training in rats
Authors: dos Santos Corrêa Moisés , dos Santos Vaz Barbara , Menezes Beatriz Scazufca , Ferreira Tatiana Lima , Tiba Paula Ayako , Fornari Raquel Vecchio 
Published: March 2021

ABSTRACTStressful and emotionally arousing experiences create strong memories that seem to lose specificity over time. It is uncertain, however, how the stress system contributes to the phenomenon of time-dependent fear generalization. Here, we investigated whether post-training corticosterone (CORT-HBC) injections, given after different training intensities, affect contextual fear memory specificity at several time points. We trained male Wistar rats on the contextual fear conditioning (CFC) task using two footshock intensities (mild CFC, 3 footshocks of 0.3 mA, or moderate CFC, 3x 0.6 mA) and immediately after the training session we administered CORT-HBC systemically. We first tested the animals in a novel context and then in the training context at different intervals following training (2, 14, 28 or 42 days). By measuring freezing in the novel context and then contrasting freezing times shown in both contexts, we inferred contextual fear generalization for each rat, classifying them into Generalizers or Discriminators. Following mild CFC training, the glucocorticoid injection promoted an accurate contextual memory at the recent time point (2 days), and increase the contextual memory accuracy 28 days after training. In contrast, after the moderate CFC training, CORT-HBC facilitated contextual generalization at 14 days, compared to the control group that maintained contextual discrimination at this timepoint. For this training intensity, however, CORT-HBC did not have any effect on recent memory specificity. These findings indicate that treatment with CORT-HBC immediately after the encoding of mild or moderately arousing experiences may differentially modulate memory consolidation and time-dependent fear generalization.HIGHLIGHTS- Rats were trained in a contextual fear task and tested for fear generalization.- Corticosterone (CORT) injected after a mild training promoted accuracy up to 28 days.- CORT injected after a moderate training facilitated time-dependent fear generalization.
Pool choice in a vertical landscape: tadpole rearing site flexibility in phytotelm-breeding frogs
Authors: Fouilloux Chloe A. , Serrano-Rojas Shirley Jennifer , Carvajal-Castro Juan David , Valkonen Janne K. , Gaucher Philippe , Fischer Marie-Therese , Pašukonis Andrius , Rojas Bibiana 
Published: March 2021

AbstractMany species of Neotropical frogs have evolved to deposit their tadpoles in small water bodies inside plant structures called phytotelmata. These pools are small enough to exclude large predators but have limited nutrients and high desiccation risk. Here, we explore phytotelm use by three common Neotropical species:Osteocephalus oophagus, an arboreal frog that periodically feeds eggs to its tadpoles;Dendrobates tinctorius, a tadpole-transporting poison frog with cannibalistic tadpoles; andAllobates femoralis, a terrestrial tadpole-transporting poison frog with omnivorous tadpoles. We found thatD. tinctoriusoccupies pools across the chemical and vertical gradient, whereasA. femoralisandO. oophagusappear to have narrower niches that are restricted primarily by pool height, water capacity, alkalinity, and salinity.Dendrobates tinctoriustadpoles are particularly flexible, and can survive in a wide range of chemical, physical, and biological conditions, whereasO. oophagusseems to prefer small, clear pools andA. femoralisoccupies medium-sized pools with abundant leaf litter and low salinity. Together, these results show the possible niche partitioning of phytotelmata among frogs, and provide insight into stressors and resilience of phytotelm breeders.
The cAMP effector PKA mediates Moody GPCR signaling inDrosophilablood-brain barrier formation and maturation
Authors: Li Xiaoling , Fetter Richard , Schwabe Tina , Jung Christophe , Steller Hermann , Gaul Ulrike 
Published: March 2021

AbstractThe blood-brain barrier (BBB) ofDrosophilais comprised of a thin epithelial layer of subperineural glia (SPG), which ensheath the nerve cord and insulate it against the potassium-rich hemolymph by forming intercellular septate junctions (SJs). Previously, we identified a novel Gi/Go protein-coupled receptor (GPCR), Moody, as a key factor in BBB formation at the embryonic stage. However, the molecular and cellular mechanisms of Moody signaling in BBB formation and maturation remain unclear. Here, we identify cAMP-dependent protein kinase A (PKA) as a crucial antagonistic Moody effector that is required for the formation, as well as for the continued SPG growth and BBB maintenance in the larva and adult stage. We show that PKA is enriched at the basal side of the SPG cell and that this polarized Moody/PKA pathway finely tunes the enormous cell growth and BBB integrity, by precisely regulating the actomyosin contractility, vesicle trafficking, and the proper SJ organization in a highly coordinated spatiotemporal manner. These effects are mediated in part by PKA’s molecular targets MLCK and Rho1. Moreover, 3D reconstruction of SJ ultrastructure demonstrates that the continuity of individual SJ segments and not their total length is crucial for generating a proper paracellular seal. Based on these findings, we propose a model that polarized Moody/PKA signaling plays a central role in controlling the cell growth and maintaining BBB integrity during the continuous morphogenesis of the SPG secondary epithelium, which is critical for maintain tissue size and brain homeostasis during organogenesis.
Novel inhibition mechanism of SARS-CoV-2 main protease by ebselen and its derivatives
Authors: Amporndanai Kangsa , Meng Xiaoli , Shang Weijuan , Jin Zhenmig , Zhao Yao , Rao Zihe , Liu Zhi-Jie , Yang Haitao , Zhang Leike , O’Neill Paul M. , Hasnain S. Samar 
Published: March 2021

AbstractThe global emergence of SARS-CoV-2 has triggered numerous efforts to develop therapeutic options for COVID-19 pandemic. The main protease of SARS-CoV-2 (Mpro), which is a critical enzyme for transcription and replication of SARS-CoV-2, is a key target for therapeutic development against COVID-19. An organoselenium drug called ebselen has recently been demonstrated to have strong inhibition against Mproand antiviral activity but its molecular mode of action is unknown preventing further development. We have examined the binding modes of ebselen and its derivative in Mprovia high resolution co-crystallography and investigated their chemical reactivity via mass spectrometry. Stronger Mproinhibition than ebselen and potent ability to rescue infected cells were observed for a number of ebselen derivatives. A free selenium atom bound with cysteine 145 of Mprocatalytic dyad has been revealed by crystallographic studies of Mprowith ebselen and MR6-31-2 suggesting hydrolysis of the enzyme bound organoselenium covalent adduct, formation of a phenolic by-product is confirmed by mass spectrometry. The target engagement of these compounds with an unprecedented mechanism of SARS-CoV-2 Mproinhibition suggests wider therapeutic applications of organo-selenium compounds in SARS-CoV-2 and other zoonoticbeta-corona viruses.
The zebrafish presomitic mesoderm elongates through compression-extension
Authors: Thomson Lewis , Muresan Leila , Steventon Benjamin 
Published: March 2021

AbstractIn vertebrate embryos the presomitic mesoderm become progressively segmented into somites at the anterior end while extending along the anterior-posterior axis. A commonly adopted model to explain how this tissue elongates is that of posterior growth, driven in part by the addition of new cells from uncommitted progenitor populations in the tailbud. However, in zebrafish, much of somitogenesis is associated with an absence of overall volume increase and posterior progenitors do not contribute new cells until the final stages of somitogenesis. Here, we perform a comprehensive 3D morphometric analysis of the paraxial mesoderm and reveal that extension is linked to a volumetric decrease, compression in both dorsal-ventral and medio-lateral axes, and an increase in cell density. We also find that individual cells decrease in their cell volume over successive somite stages. Live cell tracking confirms that much of this tissue deformation occurs within the presomitic mesoderm progenitor zone and is associated with non-directional rearrangement. Furthermore, unlike the trunk somites that are laid down during gastrulation, tail somites develop from a tissue that can continue to elongate in the absence of functional PCP signalling. Taken together, we propose a compression-extension mechanism of tissue elongation that highlights the need to better understand the role of tissue intrinsic and extrinsic forces play in regulating morphogenesis.
Handling of intracellular K+determines voltage dependence of plasmalemmal monoamine transporter function
Authors: Bhat Shreyas , Niello Marco , Schicker Klaus , Pifl Christian , Sitte Harald H. , Freissmuth Michael , Sandtner Walter 
Published: March 2021

AbstractThe concentrative power of the transporters for dopamine (DAT), norepinephrine (NET) and serotonin (SERT) is thought to be fueled by the transmembrane Na+gradient, but it is conceivable that they can also tap other energy sources, e.g. membrane voltage and/or the transmembrane K+gradient. We address this by recording uptake of endogenous substrates or the fluorescent substrate APP+((4-(4-dimethylamino)phenyl-1-methylpyridinium) under voltage control in cells expressing DAT, NET or SERT. We show that DAT and NET differ from SERT in intracellular handling of K+. In DAT and NET, substrate uptake was voltage-dependent due to the transient nature of intracellular K+binding, which precluded K+antiport. SERT, however, antiports K+and achieves voltage-independent transport. Thus, there is a trade-off between maintaining constant uptake and harvesting membrane potential for concentrative power, which we conclude to occur due to subtle differences in the kinetics of co-substrate ion binding in closely related transporters.
Learning from incidents in aircraft maintenance and continuing airworthiness: regulation, practice and gaps
Authors: Clare James , Kourousis Kyriakos I. 
Published: March 2021

Purpose
The ability to learn from previous events in support of preventing future similar events is a valuable attribute of aviation safety systems. A primary constituent of this mechanism is the reporting of incidents and its importance in support of developing learning material. Many regulatory requirements clearly define a structure for the use of learning material through organisational and procedural continuation training programmes. This paper aims to review aviation regulation and practice, highlighting the importance of learning as a key tenet of safety performance.

Design/methodology/approach
Applicable International Civil Aviation Organisation requirements and the European Union (EU) regulation in aircraft maintenance and continuing airworthiness management have been critically reviewed through content analysis.

Findings
This review has identified gaps in the European implementing rules that could be addressed in the future to support a more effective approach to the delivery of lessons in the aircraft maintenance and continuing airworthiness management sector. These include light-touch of learning and guidance requirements, lack of methodologies for the augmentation of safety culture assessment, absence of competence requirements for human factors trainers and lack of guidance on standardised root-cause analyses.

Practical implications
This paper offers aviation safety practitioners working within the European Aviation Safety Agency regulatory regime an insight into important matters affecting the ability to learn from incidents.

Originality/value
This paper evaluates critically and independently the regulation and practice that can affect the ability of EU regulated aircraft maintenance and continuing airworthiness management organisations to learn from incidents. The outputs from this research present a fresh and independent view of organisational practices that, if left unchecked, are capable of impeding the incident learning process.
What are the mechanisms through which inter-organizational relationships contribute to supply chain resilience?
Authors: Fayezi Sajad , Ghaderi Hadi 
Published: March 2021

PurposeOur study advances theory in supply chain resilience (SCRes) by identifying and describing the mechanisms through which interorganizational relationships (IORs) contribute to SCRes.Design/methodology/approachWe employ a multi-method conceptual development design combining structured and narrative review of the literature, supported by illustrative case studies. A four-stage refinement process was used for data reduction, and analysis was informed by complex adaptive systems (CAS) theory.FindingsOur findings identify connectivity, collectivity and scalability as key mechanisms through which relationships between organizations contribute to SCRes. These mechanisms draw on IOR elements of information sharing, decision synchronization and incentive alignment to augment self-organization and emergence, and adaptation and coevolution via modifying/advancing resilience strategies and practices.Originality/valueOur study advances theory and practice of SCRes by expounding on how connectivity, collectivity and scalability act as mechanisms that drive and diffuse the contribution of resilient strategies/practices to resilience capability. This is significant for strategic alignment between IORs and SCRes.
From focus to ambidexterity: the choice of catch-up strategy for EMNEs
Authors: Peng Xinmin , Fang Keyi , Lockett Martin 
Published: March 2021

PurposeEmerging-market multinational enterprises (EMNEs) can choose focused or ambidextrous strategies to catch up with global market leaders through overseas foreign direct investment (OFDI). The Belt and Road Initiative (BRI), launched by the Chinese government in 2013, had a profound impact on Chinese multinational enterprises’ international behavior. This paper analyses how EMNEs select focused or ambidextrous catch-up strategies before and after the BRI, integrating ambidexterity and catch-up theories to provide a more nuanced understanding of the evolution of EMNE strategy.Design/methodology/approachA case study is well suited for deriving rich descriptions of empirical phenomena for which little theory exists. Because the existing literature has not yet fully explored and conceptually modeled the influence of windows of opportunity on international catch-up strategies, we use qualitative research to explore the mechanisms of strategy evolution in EMNEs.FindingsThe results show that the choice of catch-up strategy is influenced by the nature of windows of opportunity and the firm's accumulated technological capability. Specifically, the opening of institutional windows as a result of the BRI could give significant momentum to the international catch-up process by providing incentives and opportunities for EMNEs to enter more markets and new technology fields. The EMNEs studied underwent a transition from a focused strategy in the catch-up stage to an ambidextrous strategy in the beyond catch-up stage.Originality/valueThese conclusions can not only deepen our understanding of the dynamics of catch-up strategies in the global context but also enrich the research on the ambidexterity of EMNEs, especially in the context of the BRI.
Bibliometric analysis of sharing economy logistics and crowd logistics
Authors: Saglietto Laurence 
Published: March 2021

Purpose
This study aims to review the literature on sharing economy logistics and crowd logistics to answer the three following questions: How is the literature on sharing economy logistics structured? What are the main trends in sharing economy logistics and crowd logistics? What are the future research options?

Design/methodology/approach
Bibliometric analysis is used to evaluate 85 articles published over the past 12 years; it identifies the top academic journals, authors and research topics contributing to the field.

Findings
The sharing economy logistics and crowd logistics literature is structured around several disciplines and highlights that some are more scientifically advanced than others in their subject definitions, designs, modelling and innovative solutions. The main trends are organized around three clusters: Cluster 1 refers to the optimal allocation of costs, prices, distribution and supplier relationships; Cluster 2 corresponds to business related crowdsourcing and international industry practices; and Cluster 3 includes the impact of transport on last-mile delivery, crowd shipping and the environment.

Research limitations/implications
The study is based on data from peer-reviewed scientific journals and conferences. A broader overview could include other data sources such as books, book chapters, working papers, etc.

Originality/value
Future research directions are discussed in the context of the evolution from crowd logistics to crowd intelligence, and the complexities of crowd logistics such as understanding how the social crowd can be integrated into the logistics process. Our results are part of the crowd science and engineering concept and provide some answers about crowd cyber-system questions regarding crowd intelligence in logistic sector.
Antecedents, processes and outcomes of an internship program: an employer's perspective
Authors: Kroon Nanja , Franco Mário 
Published: March 2021

PurposeHigher education internships have been studied in different aspects, mainly from the student or university perspective. To contribute to the understanding of the third actor in this type of university-industry cooperation, the paper examines employers' perspectives on higher education internships.Design/methodology/approachThrough a literature review and based on Narayanan et al. (2010) conceptual model, an a priori model was developed, which was then validated by a multiple-case study in a Portuguese undergraduate course.FindingsThe findings reveal rich and meaningful insights into an under-researched area, including validation of seven antecedents, two processes and six outcomes of internships from the employer's perspective. This research showed that employers give great importance to outcomes of internships, either in terms of productivity, possible future recruitment, inflow of ideas, behaviours and competences or the intern's satisfaction.Practical implicationsThe findings suggest the need for universities to undertake more collaboration with industry, as the employers made weak references to the creation and maintenance of ties with the educational institution, denoting low motivation. On the other hand, most of the outcomes for employers found in the literature are confirmed, except for the continued inflow of ideas construct. This may evidence some lack of strategic thinking related to the internship programs on part of the employers.Originality/valueThis paper validates and extends the dispersed findings of existing research by providing a useful, unifying conceptual model of the employer's perspective on higher education internships, which can be tested at other levels of education or in other countries.
The effects of a psychological brand contract breach on customers' dysfunctional behavior toward a brand
Authors: Gong Taeshik , Wang Chen-Ya 
Published: March 2021

PurposeThis paper introduces the concept of dysfunctional customer behavior toward a brand and argues that when customers perceive that a brand has failed to fulfill its promises, a psychological brand contract breach occurs, which in turn leads to a psychological brand contract violation, which evokes dysfunctional customer behavior toward the brand. In addition, this study investigates whether the impact of a breach of this contract is dependent on brand relationship quality, brand apology and restitution.Design/methodology/approachStudy 1 conducted the online survey and 224 respondents were used for data analysis and the moderating role of brand relationship quality was examined. Study 2 conducted an experiment with 201 participants to test the moderating role of brand apology and restitution.FindingsThis study found the moderating role of brand relationship quality, brand apology and brand restitution on the relationship between a psychological brand contract breach and dysfunctional customer behavior toward a brand (i.e. brand-negative word-of-mouth, brand retaliation and brand boycott), which is mediated by psychological brand contract violation.Originality/valueThis study contributes to the theoretical understanding of dysfunctional customer behavior toward a brand by integrating the literature on brand management with the organizational literature on psychological contracts between organizations and their employees. Furthermore, this study sheds light on the effectiveness of reparative actions by the firm after occurrence of the psychological brand contract breach.