bluuebunny/crossref_metadata_2025_split huggingface dataset:
Lean Adoption in Hospitals: The Role of Contextual Factors and Introduction Strategy
Authors: Rosa Angelo , Marolla Giuliano , Lega Federico , Manfredi Francesco 
Published: March 2021

Abstract
Background: In the scientific literature, many studies describe the application of lean methodology in the hospital setting. Most of the articles focus on the results rather than on the approach adopted to introduce the lean methodology. In the absence of a clear view of the context and the introduction strategy, the first steps of the implementation process can take on an empirical, trial and error profile. Such implementation is time-consuming and resource-intensive and affects the adoption of the model at the organizational level. This research aims to outline the role contextual factors and introduction strategy play in supporting the operators introducing lean methodology in a hospital setting. Methodology: The methodology is revealed in a case study of an important hospital in Southern Italy, where lean has been successfully introduced through a pilot project in the pathway of cancer patients. The originality of the research is seen in the detailed description of the contextual elements and the introduction strategy. Results: The results show significant process improvements and highlight the spontaneous dissemination of the culture of change in the organization and the streamlined adoption at the micro level. Conclusion: The case study shows the importance of the lean introduction strategy and contextual factors for successful lean implementation. Furthermore, it shows how both factors influence each other, underlining the dynamism of the organizational system.
Construction of a circRNA-miRNA-mRNA network based on competitive endogenous RNA reveals the key pathways and central genes of osteosarcoma in vivo
Authors: Chen Zhihao , Wang Xi , Li Liubing , Han Mingxiao , Wang Min , Hu Gangfeng , Xie Zonggang , Zhang Haifang 
Published: March 2021

Abstract
Circular RNAs (circRNAs) play important roles in a variety of pathological functions. However, the potential functions and detailed mechanisms of circRNAs in osteosarcoma (OS) have not been fully elucidated. In this study, the circRNA, micro RNA (miRNA), and messenger RNA (mRNA) expression profile of human OS was investigated based on the raw microarray data GSE140256, GSE65071 and GSE16088 in Gene Expression Omnibus (GEO) datasets, and seven differentially-expressed circRNAs (DEcircRNAs), 166 differentially-expressed miRNAs (DEmiRNAs), and 175 differentially-expressed mRNAs (DEmRNAs) were identified in total. FunRich was employed to analyze the differentially-expressed transcription factors on the basis of identified DEmiRNAs. In addition, the Gene Ontology (GO) annotation and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis were used to further study biological functions of the DEmRNAs. Interestingly, post-translational protein modification, collagen-containing extracellular matrix, and single-stranded DNA binding were the most significant pathways enriched for DEmRNAs in GO annotation analysis. Meanwhile, in KEGG pathway enrichment analysis, complement and coagulation cascades, RNA transport and drug metabolism − other enzymes were the most significantly enriched pathways of DEmRNAs in OS. We constructed circRNA-miRNA-mRNA and protein–protein interaction (PPI) networks that may be associated with pathological processes of OS. Finally, we also revealed the pattern of tumor-infiltrating immune cells in OS and further explored the ceRNA networks we constructed in which we found that COL1A1 and RAN were significantly correlated with overall survival in patients with osteosarcoma (p < 0.05). To our knowledge, this study provides the first profile analysis of DEcircRNAs, DEmiRNAs, and DEmRNAs with OS in vivo and reveals a novel idea for understanding the pathogenesis of OS.
Integrated analysis of Transcriptomic Data Reveal AP-1 as a Potential Regulation Hub in Inflammation Induced Hyperalgesia Rat
Authors: Zhu Xiang , Li Feng , Wang Miqun , Su Huibin , Wu Xuedong , Qiu Haiyan , Zhou Wang , Shan Chunli , Wang Cancan , Wei Lei 
Published: March 2021

Abstract
BackgroundInflammation associated chronic pain is a global clinical problem, affecting millions of people worldwide. However, the underlying mechanism of inflammation associated chronic pain remains unclear. Complete Freund's adjuvant (CFA) induced cutaneous inflammation rat has been widely used as an inflammation-induced pain hypersensitivity model.MethodsHerein, we presented a transcriptomic profile of CFA-induced rat dorsal root ganglion via an approach targeting gene expression, DNA methylation and post-transcriptional regulation.ResultsWe identified 418 differentially expressed mRNAs, 120 differentially expressed microRNAs and 2670 differentially methylated regions, which are all highly associated with multiple inflammation related pathways, including NF-κB signaling pathways and IFN signaling pathways. Integrated analysis further demonstrated that AP-1 network, which may work as a regulator of inflammation response, is sophisticatedly regulated at both transcriptomic and epigenetic level.ConclusionsWe believe our data will not only provide drug screening targets for treating chronic pain and inflammation, but also shed a light on the molecular network inflammation induced hyperalgesia.
Ferric sulphate flocculation as a concentration method for Giardia and Cryptosporidium in filter backwash water
Authors: Silva Kamila Jessie Sammarro , Sabogal-Paz Lyda Patricia 
Published: March 2021

Abstract
Filter backwash water (FBW) is a prominent residue from water treatment plants (WTPs) that is often disposed into water bodies or recycled within the WTP without due disinfection. FBW usually contains particles within a size range that includes pathogenic protozoa, as the infective forms of Giardia and Cryptosporidium, parasites responsible for waterborne diseases outbreaks. Quantifying (oo)cysts is essential for addressing this matter, as it might assist research on giardiasis and cryptosporidiosis epidemiology, as well as shed light onto disinfection technologies for FBW. However, (oo)cyst recovery from FBW and other complex matrices still lacks a standard protocol and entails specialized professionals and expensive material. Seeking to provide insight in a reduced-cost recovery method, this study analysed the recovery efficiency (RE) obtained by acid flocculation with ferric sulphate, a common coagulant, on bench-scale simulated FBW. Steps included concentration by flocculation, centrifugation, and quantification by immunofluorescence. Although recovery was sufficient for Cryptosporidium parvum (40.59%), Method 1623.1 recommendations were not reached for Giardia muris (1.76%). Coefficients of variation obtained for both organisms were not satisfactory, highlighting the variability to which environmental matrices are subjected and why defining a methodology for (oo)cyst recovery in WTP residues is important.
A HPLC-DAD-MS/MS Method for Simultaneous Determination of Six Active Ingredients of Salviae Miltiorrhizae and Ligustrazine Hydrochloride Injection in Rat Plasma and its Application to Pharmacokinetic Studies
Authors: Pan Jianyang , Zhang Luquan , Xiong Difeifei , Li Bailing , Qu Haibin 
Published: March 2021

Aims:
This is a pharmacokinetic study of Salviae miltiorrhizae and ligustrazine hydrochloride injection.
The study aimed to evaluate the mechanism of action, safety and rational clinical use of Salviae miltiorrhizae and
ligustrazine hydrochloride injection.

Background:
Salviae miltiorrhizae and ligustrazine hydrochloride injection is a compound preparation consisted of
Salvia miltiorrhiza extract and ligustrazine hydrochloride for the treatment of cardiovascular and cerebrovascular
diseases in China.

Objective:
The study aimed to develop a rapid and sensitive high-performance liquid chromatography-diode array
detector-tandem mass spectrometry (HPLC-DAD-MS/MS) method for simultaneous determination of six major
active ingredients of Salviae miltiorrhizae and ligustrazine hydrochloride injection, namely danshensu, protocatechuic
aldehyde, rosmarinic acid, lithospermic acid, salvianolic acid A, and ligustrazine hydrochloride, in rat plasma.

Methods:
Plasma samples were precipitated with methanol, which was spiked with ascorbic acid and the supernatant
was separated on a Waters Cortecs C18 column, by using a gradient mobile phase system of acetonitrile-water containing
0.05% formic acid (v/v). For internal standards, puerarin was selected for the five salvianolic acids, while
isofraxidin was used for ligustrazine hydrochloride. Besides, electrospray ionization in negative mode and multiplereaction
monitoring were used to identify and quantify the five salvianolic acids, whereas ligustrazine hydrochloride
was quantified at 310 nm using the diode array detector.

Results:
Noticeably, all calibration curves showed good linearity (R2>0.99) over the concentration range, with a
lower limit of quantification between 0.00411 and 0.0369 μg/mL for salvianolic acids, and 1.74 μg/mL for ligustrazine
hydrochloride. Next, the precision of the developed method was evaluated by intra- and inter-day assays, and
the percentage of relative standard deviation was within 10%. Although the extraction efficiency of some salvianolic
acids was not very satisfactory, the sensitivity of the analytical method met the analysis requirements of rat plasma
samples. Moreover, the validated method was successfully applied to a pharmacokinetic study of Salviae miltiorrhizae
and ligustrazine hydrochloride injection in the rat model.

Conclusion:
Linear pharmacokinetic characteristics were observed for the six active ingredients after intravenous
infusion administration in rats within the dose range examined here. In summary, our study proposed a HPLC-DADMS/
MS method with the simultaneous determination of multiple ingredients, and demonstrated its applicability in
pharmacokinetic studies.
CHEMICAL AND BIOLOGICAL ANALYSIS OF THE BIOCTIVE FRACTIONS OF THE LEAVES OF SCAEVOLA TACCADA (GAERTN.) ROXB
Authors: GHANI EMAN M. ABDEL , SAYED ABEER M. EL , TADROS SOAD H. , SOLIMAN FATHY M. 
Published: March 2021

Objective: Scaevola taccada. (Gaertn.) Roxb. is widely dispersed all along the coasts of Africa. It is used in folk medicine for diversity of ailments. This study aims to investigate the major phytoconstituents and biological activities of the leaves of S. taccada (Gaertn.) Roxb.
Methods: In vitro biological examination viz. antimicrobial, cytotoxic and antioxidant activities of the ethanol extract of the leaves (EE) and its fractions; (petroleum ether (PE), methylene chloride (MC), ethyl acetate (EA) and n-butanol(BuOH)) were carried out. Estimation of the phytochemicals of biologically active fractions was done.
Results: n-butanol fraction displayed remarkable antimycobacterium activity. Petroleum ether as well as n-butanol fractions evidenced a cytotoxic effect on breast carcinoma cell line (MCF7) and colon carcinoma cell line (HCT) with IC50 11.7 and 15.04 µg/ml respectively. Moreover, ethyl acetate fraction exhibits an antioxidant effect with EC50 476.7±0.57 µg/ml. n-tetradecane 1, α-amyrin palmitate 2, α-amyrin acetate 3, α-amyrin 4, stigmasterol 5, luteolin-7-O-β-glucoside 6, rutin 7 and alidyjosioside 8 were identified in S. taccada (Gaertn.) Roxb. leaves.
Conclusion: Petroleum ether fraction is a cytotoxic candidate, especially against (MCF-7). It exhibited a moderate antifungal and antibacterial against certain Gram-positive bacteria. Ethyl acetate showed an antioxidant effect along with moderate antifungal activity. n-butanol fraction exerted potential antimycobacterial, significant cytotoxic activity against (HCT), good antifungal and antibacterial against Gram-positive and Gram-negative bacteria. Stigmasterol, luteolin-7-O-β-glucoside, rutin and alidyjosioside were isolated for the first time from S. taccada (Gaertn.) Roxb. Leaves.
Sobre la misteriosa invención de los psicotrópicos
Authors: Paláu Castaño Luis Alfonso 
Published: March 2021

Traducción del francés al español de Luis Alfonso Paláu-Castaño
«Hay pues, ciertamente, “alguna cosa” común en ratas, ratones, perros y seres humanos, más allá del soma, o a partir del soma. Después de todo, se puede tener confianza en la industria farmacéutica al menos en un punto: solo el resultado cuenta. Pero se está sobre una falsa pista si se cree que esa “cosa” está determinada por adelantado, que depende de una teoría de los animales, por ejemplo, de lo que tienen de común y de diferente con la especie humana. De hecho, esa “alguna cosa” que circula entre las ratas y los humanos no está dada por adelantado en las experiencias de los farmacólogos. Está en perpetua invención. Por lo demás, esta es la razón por la cual es tan difícil establecerla y caracterizarla.»
Lorenzo Fernândez: considerações sobre sua contribuição à Música Brasileira da primeira metade do Século XX
Authors: Barros José D'Assunção 
Published: March 2021

Este artigo propõe a apresentar e discutir a obra musical de Lorenzo Fenandez, considerando-a do ponto de vista de sua possível divisão em fases e orientando a análise em torno de aspectos que incluem (1) o modelo de utilização do folclore musical empregado em suas obras nacionalistas; (2) a especificidade formal dos padrões composicionais de Lorenzo Fernândez e sua utilização de formas musicais diversas; (3) a variedade de gêneros musicais percorrida pela atividade composicional deste autor AbstractThis article aims to discuss the musical work of Lorenzo Fenândez, examining it on the perspective on the possibility of considering this work in different phases and directing the analysis around aspects that include (1) the pattern of utilization of the musical folklore used in his nationalistic works, (2) the formal specificity of the compositional models of Lorenzo Fenândez and their utilization in various musical forms, (3) the variety of musical genders experienced by the compositional activity of the author.
A Grade
Authors: Caló Susana 
Published: March 2021

Este ensaio enceta uma discussão em torno do protocolo experimental chamado 'grille', desenvolvido na clínica La Borde fundada por Jean Oury em 1952. O ensaio aborda o projecto da psicoterapia institucional refletido na grade. Oferece uma visão da grade como um instrumento de criação coletiva contínua da instituição de cuidado, com efeitos terapêuticos para todas as pessoas envolvidas, desde o pessoal até aos residentes e monitores. Descrevendo como a grade funcionava através da criação de ligações entre o material e o semiótico, o mental e o organizacional, o social e o ambiental, com o efeito de não só singularizar a trajetória da própria instituição, mas também daqueles que nela vivem, o ensaio salienta a importância dos processos analíticos institucionais em projectos emancipatórios. AbstractThis text presents a close discussion of the experimental protocol called ‘grid’ as developed at the clinic La Borde founded by Jean Oury in 1952. The paper teases out the institutional psychotherapy project reflected in the grid. It offers a view of the grid as an instrument of continuous collective creation of the institution of care, with therapeutic effects to all persons involved, from staff to residents and monitors. Describing how the grid worked by the creation of connections between the material and the semiotic, the mental and the organisational, the social and the environmental, to the effect of not only singularising the trajectory of the institution itself, but also of those living in it, the paper highlights the importance of institutional analytical processes in emancipatory projects.
Adapting a Classroom Simulation Experience to an Online Escape Room in Nutrition Education
Authors: Ross Jenifer , Wright Lauri , Arikawa Andrea 
Published: March 2021

Due to the COVID-19 emergency transition to remote learning, an undergraduate class in nutrition and dietetics modified a face-to-face experiential “escape room” assignment into a comparable online experience. The online assignment was structured so that students had to use knowledge and clues to move through each step of the Nutrition Care Process; students proceeded through the escape room individually until each successfully “escaped.” An important component of this assignment was the postactivity debriefing process, which took place via video conferencing in small groups. Students indicated that they were pleasantly surprised at the effectiveness of the online assignment. However, analytics showed that students progressed through most of the steps fairly quickly; thus, instructors plan to improve future deployments by using a variety of interactive assessments and adding more layered criteria and clues within each of the escape room steps.
Diasporic Dilemma in Amit Chaudhuri’s Afternoon Raag
Authors: Sadath K Asfar 
Published: March 2021

Identity is one of the important themes of the diasporic writing. Identity plays an important role in an immigrant's life because they feel rootless and nostalgic when they try to become members of a new group. There are different aspects of identities like political, social, cultural, economic and individual and so on. These are playing an important role in an immigrant's life. The concept of home always gives a sweet feel for immigrants. A sense of belongingness plays an important role in immigrant life. Immigrants never accept the host country as their country.
The Paradox of Integrity: Cheating Awareness Among Religious High School Student in Yogyakarta [Paradoks Integritas: Kesadaran akan Perilaku Kecurangan di Kalangan Siswa Sekolah Menengah Atas Berbasis Agama di Yogyakarta]
Authors: Jamaluddin Samudera Fadlilla , Lufityanto Galang 
Published: March 2021

Cheating behavior in a school setting has become a major problem in many countries, including those with a dominantly religious population (e.g., Indonesia). This creates a paradox since previous literatures suggest eminent role of spirituality in constructing morality. This study is intended to investigate the interplay between spirituality and cheating behavior in religious high school students in Yogyakarta using multi-methods approach. Self-report questionnaires to measure the perception toward cheating, spirituality level, and locus of control were distributed to a total of 691 participants using random sampling method. Participants were also given an open-ended questionnaire asking the rationale behind their past cheating behavior. The findings suggested that the role of spirituality in ethical behavior was rather contextual, such as the link between spirituality and awareness of cheating was more robust in the respondents from religious schools. It was found that the participants viewed spirituality as an internal moral compass which may in turn facilitate overt ethical behavior.

Perilaku kecurangan di sekolah telah menjadi permasalahan besar di berbagai negara, termasuk di negara-negara yang mayoritas penduduknya religius (misalnya di Indonesia). Hal ini menimbulkan sebuah paradoks mengingat penelitian terdahulu menyatakan bahwa spiritualitas berperan dalam proses pembentukan moralitas. Penelitian ini bertujuan untuk menelaah interaksi antara spiritualitas dan perilaku kecurangan pada siswa sekolah menengah di Yogyakarta menggunakan pendekatan multi-metode. Kuesioner laporan diri untuk mengukur persepsi terhadap kecurangan, tingkat spiritualitas, dan lokus kendali dibagikan kepada total 691 partisipan menggunakan metode random sampling. Partisipan juga diberikan kuesioner dengan butir-butir pertanyaan terbuka yang menanyakan alasan di balik perilaku curang mereka di masa lampau. Temuan menunjukkan bahwa peran spiritualitas dalam perilaku etis cenderung bersifat kontekstual, misalnya hubungan antara spiritualitas dan kesadaran akan perilaku kecurangan lebih kuat pada responden dari sekolah berbasis agama. Ditemukan bahwa partisipan dalam kajian ini memandang spiritualitas sebagai kompas moral internal yang dapat berperan dalam memfasilitasi perilaku etis yang terbuka.
The Efficacy of Think Talk Write Strategy in Improving Writing Skill for Teeneagers at Batu Walenrang Palopo
Authors: Masruddin Masruddin , Furwana Dewi , Jafar Abdul 
Published: March 2021

This research about the efficacy of think talk write strategy in improving writing skill for teenagers at Batu Walenrang Palopo. The purpose of this research is to find out whether or not the use of think talk write strategy is effective in improving writing skill for teenagers (16th-17th) at Batu Walenrang Palopo. This research applied the pre-experimental method, the research is carried out in one class, the population of this research is teenagers at Batu Walenrang Palopo. The sample of this research are nine teenagers (16th-17th) at Batu Walenrang Palopo. The sampling technique in this research is the total sampling technique. The instrument of this research is writing test. The researcher gives pretest and post-test to the teenagers. the data are analyzed using Spss 20. The result of this research shows that the use of think talk write strategy is effective in writing. It could be proved from the result of the calculation that students score in the post-test is higher than students score in the pre-test. The mean score in Post-test is higher than the mean score of the pre-test P=0.003>0.005. It means that is the use of think talk write strategy is effective in improving writing skill for teenagers (16th-17th) at Batu Walenrang Palopo. The implementation of this research as an alternative for educators in developing students' understanding in learning.
PENGARUH PENERAPAN STRATEGI PEMBELAJARAN KONSTRUKTIVISTIK PBP (PEMBELAJARAN BERBASIS PROYEK) DAN TINGKAT KEMAMPUAN AKADEMIK TERHADAP SIKAP SISWA SMA KELAS X DI MALANG PADA EKOSISTEM SUNGAI
Authors: Iqbal Mochammad 
Published: March 2021

Penelitian ini bertujuan untuk: (1) mengetahui pengaruh penerapan strategi pembelajaran berbasis konstruktivistik PBP terhadap sikap siswa SMA di Malang pada ekosistem sungai, (2) mengetahui pengaruh tingkat kemampuan akademik terhadap sikap siswa SMA di Malang pada ekosistem sungai, dan (3) mengetahui pengaruh interaksi antara penerapan strategi pembelajaran berbasis konstruktivistik PBP dan tingkat akademik terhadap sikap siswa SMA di Malang pada ekosistem sungai.
Sampel dalam penelitian ini adalah siswa kelas X7 dan X8 SMAN 2 Malang serta X2 dan X6 SMA Laboratorium Universitas Negeri Malang. Penelitian ini berlangsung antara tanggal 14 Maret – 31 mei 2009. Data dianalisis menggunakan analisis statistik kovarian (ANAKOVA) dan dilanjutkan dengan uji lanjut LSD.
Hasil penelitian ini, menunjukkan bahwa PBP berpengaruh nyata secara statistik terhadap sikap siswa pada ekosistem sungai, siswa yang difasilitasi dengan PBP memberikan hasil nilai sikap lebih tinggi 9,9% dari pada multistrategi, sedangkan kemampuan akademik serta interaksi PBP dan kemampuan akademik tidak berpengaruh terhadap sikap siswa pada ekosistem sungai.
PENGARUH PEMBELAJARAN PROJECT BASED LEARNING (PBL) PADA MATERI EKOSISTEM TERHADAP SIKAP DAN HASIL BELAJAR SISWA SMAN 2 MALANG
Authors: Darmawan Ericka 
Published: March 2021

Penelitian ini untuk mengetahui pengaruh PBL terhadap sikap dan hasil belajar siswa SMAN 2 Malang. Jenis penelitian menggunakan eksperimen semu atau quasi eksperiment, non equivalent control group design. Siswa yang diajar dengan pembelajaran berbasis proyek memiliki sikap lebih tinggi 11,65% dari peserta didik yang diajar dengan pembelajaran konvensional. Siswa yang difasilitasi pembelajaran berbasis proyek memiliki pemahaman konsep lebih tinggi 81,05% dari siswa yang diajar dengan pembelajaran konvensional. Untuk itu, dalam meningkatkan hasil belajar siswa hendaknya guru memilih strategi pembelajaran yang memungkinkan siswa aktif mengkonstruk pengetahuan sendiri, untuk meningkatkan pemberdayaan sikap siswa terhadap lingkungan hidup, guru dalam mendesain pembelajaran yang memungkinkan siswa menggunakan kognitif tingkat tinggi untuk pemecahan masalah terkait lingkungan hidup.