bluuebunny/crossref_metadata_2025_split huggingface dataset:
Measuring Electronic Health Record Use in the Pediatric ICU Using Audit-Logs and Screen Recordings
Authors: Sinha Amrita , Stevens Lindsay A. , Su Felice , Pageler Natalie M. , Tawfik Daniel S. 
Published: August 2021

Abstract
Background Time spent in the electronic health record (EHR) has been identified as an important unit of measure for health care provider clinical activity. The lack of validation of audit-log based inpatient EHR time may have resulted in underuse of this data in studies focusing on inpatient patient outcomes, provider efficiency, provider satisfaction, etc. This has also led to a dearth of clinically relevant EHR usage metrics consistent with inpatient provider clinical activity.
Objective The aim of our study was to validate audit-log based EHR times using observed EHR-times extracted from screen recordings of EHR usage in the inpatient setting.
Methods This study was conducted in a 36-bed pediatric intensive care unit (PICU) at Lucile Packard Children's Hospital Stanford between June 11 and July 14, 2020. Attending physicians, fellow physicians, hospitalists, and advanced practice providers with ≥0.5 full-time equivalent (FTE) for the prior four consecutive weeks and at least one EHR session recording were included in the study. Citrix session recording player was used to retrospectively review EHR session recordings that were captured as the provider interacted with the EHR.
Results EHR use patterns varied by provider type. Audit-log based total EHR time correlated strongly with both observed total EHR time (r = 0.98, p < 0.001) and observed active EHR time (r = 0.95, p < 0.001). Each minute of audit-log based total EHR time corresponded to 0.95 (0.87–1.02) minutes of observed total EHR time and 0.75 (0.67–0.83) minutes of observed active EHR time. Results were similar when stratified by provider role.
Conclusion Our study found inpatient audit-log based EHR time to correlate strongly with observed EHR time among pediatric critical care providers. These findings support the use of audit-log based EHR-time as a surrogate measure for inpatient provider EHR use, providing an opportunity for researchers and other stakeholders to leverage EHR audit-log data in measuring clinical activity and tracking outcomes of workflow improvement efforts longitudinally and across provider groups.
A novel taxonomy of smart sustainable city indicators
Authors: Pira Milad 
Published: August 2021

AbstractBuilding a smart city that follows sustainability goals enhances the quality of life and preserves environmental, human, and social capital. Yet, existing smart sustainable city projects have concentrated on the technological dimensions of smart cities such as using big data or smart devices to follow sustainability goals. Currently, there is no comprehensive category of smart sustainable city indicators in the literature. This paper aims to discover these indicators by considering the common features of sustainability and smart city concepts. Two rounds of the content analysis technique were employed to investigate semantic, lexical, and conceptual relationships between smart city and sustainability indicators. This paper employed the Sustainable Development Indicators suggested by OECD and the Smart City Index Master by Cohen as the two main groups of indicators. The findings offer a novel set of indicators that enables policymakers and researchers to consider the smartness and sustainability of their projects simultaneously. This includes socio-cultural, economic, environmental, and governance categories with 28 associated indicators. The outcome of this paper offers a unique combined category of smart sustainable city indicators by considering the key elements of sustainability and smart city concepts. Academics and policymakers can also employ this set of indicators as a guideline to build a smart sustainable community.
Success factors for a Mandarin bilingual program
Authors: Liu Wei 
Published: August 2021

Abstract
Immersion-style bilingual school programs have proven to be effective ways for children’s acquisition of additional languages. However, such programs seem to be scarce across the world in comparison to the value people attach to bilingual skills and bilingual education. Through a case study of a successful Mandarin bilingual program in western Canada, the study hopes to explore the factors that contribute to the success of such programs in North America. The study points to the importance of government language policy environments, the attitude of local school boards, the commitment and active involvement of parents, and the availability and recruitment of quality teachers as key factors for the success of a bilingual school program.
Food safety and methods to ensure food security in the face of climate change
Authors: Tripathi Jyoti , Variyar Prasad S. 
Published: August 2021

Abstract
Serious social, economic, and ecological consequences of climate change due to the high levels of greenhouse gases (GHGs) in our atmosphere resulting from a wide range of human activities including the burning of fossil fuels and land use have impacted weather events world over. Extreme weather events and warmer global temperatures are likely to be more frequent with an adverse overall effect on agricultural production unless there is an urgent reduction in GHG emissions. There is thus an immediate need for increasing adaptive capacity in agriculture to long-term climatic trends and increasing variability in weather patterns. Climate change also poses significant challenges to global food safety due to the emergence of new pathogens, insect pests, and toxicants. Food safety threats cause an enormous burden on economies due to disruptions or restrictions in global and regional agrifood trade, food loss, and associated income. Food safety thus plays a critical role across the four pillars of food security—availability, access, utilization, and stability. Climate change is likely to create new safety issues entailing reassessing our tolerance to risk and safety limits presently established for the human food chain. The present review focuses on the factors affecting food security and safety as a consequence of climate change and the pre- and postharvest strategies that need to be adopted to mitigate these effects for enhancing food safety and global food sufficiency in future.
Electric trapping and circuit cooling of charged nanorotors
Authors: Martinetz Lukas , Hornberger Klaus , Stickler Benjamin A 
Published: August 2021

Abstract
The motion of charged particles can be interfaced with electric circuitry via the current induced in nearby pick-up electrodes. Here we show how the rotational and translational dynamics of levitated objects with arbitrary charge distributions can be coupled to a circuit and how the latter acts back on the particle motion. The ensuing cooling rates in series and parallel RLC circuits are determined, demonstrating that quadrupole ion traps are well suited for implementing all-electric cooling. We derive the effective macromotion potential for general trap geometries and illustrate how consecutive rotational and translational resistive cooling of a microscale particle can be achieved in linear Paul traps.
Bio-3D printing iPSC-derived human chondrocytes for articular cartilage regeneration
Authors: Nakamura Anna , Murata Daiki , Fujimoto Ryota , Tamaki Sakura , Nagata Sanae , Ikeya Makoto , Toguchida Junya , Nakayama Koichi 
Published: August 2021

Abstract
Osteoarthritis is a leading cause of pain and joint immobility, the incidence of which is increasing worldwide. Currently, total joint replacement is the only treatment for end-stage disease. Scaffold-based tissue engineering is a promising alternative approach for joint repair but is subject to limitations such as poor cytocompatibility and degradation-associated toxicity. To overcome these limitations, a completely scaffold-free Kenzan method for bio-3D printing was used to fabricate cartilage constructs feasible for repairing large chondral defects. Human induced pluripotent stem cell (iPSC)-derived neural crest cells with high potential to undergo chondrogenesis through mesenchymal stem cell differentiation were used to fabricate the cartilage. Unified, self-sufficient, and functional cartilaginous constructs up to 6 cm2 in size were assembled by optimizing fabrication time during chondrogenic induction. Maturation for 3 weeks facilitated the self-organisation of the cells, which improved the construct’s mechanical strength (compressive and tensile properties) and induced changes in glycosaminoglycan and type II collagen expression, resulting in improved tissue function. The compressive modulus of the construct reached the native cartilage range of 0.88 MPa in the 5th week of maturation. This paper reports the fabrication of anatomically sized and shaped cartilage constructs, achieved by combining novel iPSCs and bio-3D printers using a Kenzan needle array technology, which may facilitate chondral resurfacing of articular cartilage defects.
Study of bleaching of old newsprint recycled paper: reproduction of newspaper material
Authors: Zeb Hassan , Hussain Muhammad Asif , Ahmed Iftikhar , Akram Muhammad Sarfraz , Haider Bilal , Haider Rizwan , Babar Zaeem Bin , Saleem Rana Mujahid , Ahsan Abuzar , Aziz Iqra , Arif Muhammad 
Published: August 2021

Abstract
With the utilization of used newspaper into the paper making industry, bleaching has been an area of concern to obtain a balance between desired brightness and the amount of treatment it requires. Motivation of this study was to optimize bleaching of recycled newsprint fibers in terms of five variables namely hydrogen peroxide charge, amount of sodium hydroxide employed, pulp consistency, bleaching temperature, and bleaching time. Experimental work was conducted in the laboratory to simulate bleaching plant and it was found that higher peroxide charge is better for improvement in brightness, but it is uneconomical to increase peroxide charge indefinitely. The amount of hydrogen peroxide is added so that the residual peroxide at the end of the reaction is not less than 10% of the initially added peroxide. Sodium hydroxide provides alkalinity which is very important for making the fibers flexible and softening of ink particles but it also contributes to pulp yellowing if residual hydrogen peroxide contents are lesser than the minimum required value. So, for 16 kg t−1 of hydrogen peroxide, 6 kg t−1 of sodium hydroxide prevented the brightness reversion in this study. Higher pulp consistency is better but unavailability of very high consistency pulp mixer in the industry is a limiting factor for higher than 30% pulp consistency. Temperature and time are quite interrelated and, with the given bleaching conditions, a temperature of 95 °C with 10 min residence time was found to be optimum for this study. It was also observed that multiple pre-washings increase brightness and save bleaching chemicals.
Preparation and in vitro release of total alkaloids from alstonia scholaris leaf-loaded mPEG-PMA microspheres
Authors: Jiang Nianfeng , Zheng Xiangyu , Feng Yan , Wu Hongtao , Yuan Mingwei , He Yi , Li Hongli , Yuan Minglong 
Published: August 2021

Abstract
The total alkaloids extracted from the leaves of Alstonia scholaris (ASAs) have been reported to reduce fever, remove phlegm, and relieve coughs. However, their drug half-lives are short. Thus, to obtain sustained-release preparations of total alkaloids from ASAs, mandelic acid oxyanhydride (mandelic acid OCA) was synthesized by the reaction of L-mandelic acid (MA) with triphosgene, and subsequent copolymerization with polyethylene glycol monomethyl ether (mPEG) of different molecular weights yielded the corresponding mPEG poly-MA (mPEG-PMA) copolymers. ASAs-loaded microspheres were then prepared using the double emulsion method, and their in vitro release (15 d, 37 °C) and in vitro degradation behaviors were studied. The morphology, size, embedding efficiency, and drug loading efficiency were investigated for the prepared microspheres, and screening was carried out using the mPEG10K-PMA drug-loaded microspheres to analyze their biological characteristics. Anti-inflammatory experiments using Kunming mice and Sprague Dawley rats showed that the microspheres exhibited good anti-inflammatory properties. Moreover, the ASAs-loaded microspheres exhibited a good biocompatibility, and the hemolysis rate was <5%.
Hyaluronic acid-based supramolecular hydrogels for biomedical applications
Authors: Mihajlovic Marko , Fermin Liline , Ito Keita , van Nostrum Cornelus F , Vermonden Tina 
Published: August 2021

Abstract
Soft materials, such as hydrogels, are used as scaffolds in tissue engineering and regenerative medicine to help tissues regenerate and heal. Recently, supramolecular hydrogels, based on non-covalent interactions, have grown in popularity, especially in the development of materials for biomedical use. Their potential lies in the dynamic, reversible and temporary nature of their crosslinks, which can make them responsive to stimuli, injectable and suitable for 3D printing. Such versatility and processability is important when developing new biomaterials for drug delivery or as implantable scaffolds. The behavior and properties of such hydrogels are different compared to those of chemically crosslinked hydrogels. In this review, we give an overview on supramolecular hydrogels which contain hyaluronic acid (HA) as one of the building blocks. HA is particularly interesting, due to its hydrophilicity, biofunctionality and ease of chemical modification. Specifically, we focus on HA-based hydrogels that make use of hydrogen bonding, hydrophobic interactions, electrostatic interactions, metal–ion coordination and guest–host interactions, and are intended for applications in the biomedical field, with potential for clinical translation.
A Tale of Two Approaches
Authors: Ghayad Jean Pierre E , Barakett-Hamadé Vanda P 
Published: August 2021

Abstract

Objectives
To summarize and assess the literature on the performances of methods beyond the Friedewald formula (FF) used in routine practice to determine low-density lipoprotein cholesterol (LDL-C).

Methods
A literature review was performed by searching the PubMed database. Many peer-reviewed articles were assessed.

Results
The examined methods included direct homogeneous LDL-C assays, the FF, mathematical equations derived from the FF, the Martin-Hopkins equation (MHE), and the Sampson equation. Direct homogeneous assays perform inconsistently across manufacturers and disease status, whereas most FF-derived methods exhibit variable levels of performance across populations. The MHE consistently outperforms the FF but cannot be applied in the setting of severe hypertriglyceridemia. The Sampson equation shows promise against both the FF and MHE, especially in severe hypertriglyceridemia, but data are still limited on its validation in various settings, including disease and therapeutic states.

Conclusions
There is still no consensus on a universal best method to estimate LDL-C in routine practice. Further studies are needed to assess the performance of the Sampson equation.
The influence of maternal glucocorticoids on offspring phenotype in high- and low-risk environments
Authors: MacLeod Kirsty J , Langkilde Tracy , Venable Cameron P , Ensminger David C , Sheriff Michael J 
Published: August 2021

Abstract
Elevated maternal glucocorticoid levels during gestation can lead to phenotypic changes in offspring via maternal effects. Although such effects have traditionally been considered maladaptive, maternally derived glucocorticoids may adaptively prepare offspring for their future environment depending upon the correlation between maternal and offspring environments. Nevertheless, relatively few studies test the effects of prenatal glucocorticoid exposure across multiple environments. We tested the potential for ecologically relevant increases in maternal glucocorticoids in the eastern fence lizard (Sceloporus undulatus) to induce adaptive phenotypic changes in offspring exposed to high or low densities of an invasive fire ant predator. Maternal treatment had limited effects on offspring morphology and behavior at hatching, but by 10 days of age, we found maternal treatment interacted with offspring environment to alter anti-predator behaviors. We did not detect differences in early-life survival based on maternal treatment or offspring environment. Opposing selection on anti-predator behaviors from historic and novel invasive predators may confound the potential of maternal glucocorticoids to adaptively influence offspring behavior. Our test of the phenotypic outcomes of transgenerational glucocorticoid effects across risk environments provides important insight into the context-specific nature of this phenomenon and the importance of understanding both current and historic evolutionary pressures.
Co-evolutionary distance predictions contain flexibility information
Authors: Schwarz Dominik , Georges Guy , Kelm Sebastian , Shi Jiye , Vangone Anna , Deane Charlotte M 
Published: August 2021

Abstract

Motivation
Co-evolution analysis can be used to accurately predict residue–residue contacts from multiple sequence alignments. The introduction of machine-learning techniques has enabled substantial improvements in precision and a shift from predicting binary contacts to predict distances between pairs of residues. These developments have significantly improved the accuracy of de novo prediction of static protein structures. With AlphaFold2 lifting the accuracy of some predicted protein models close to experimental levels, structure prediction research will move on to other challenges. One of those areas is the prediction of more than one conformation of a protein. Here, we examine the potential of residue–residue distance predictions to be informative of protein flexibility rather than simply static structure.

Results
We used DMPfold to predict distance distributions for every residue pair in a set of proteins that showed both rigid and flexible behaviour. Residue pairs that were in contact in at least one reference structure were classified as rigid, flexible or neither. The predicted distance distribution of each residue pair was analysed for local maxima of probability indicating the most likely distance or distances between a pair of residues. We found that rigid residue pairs tended to have only a single local maximum in their predicted distance distributions while flexible residue pairs more often had multiple local maxima. These results suggest that the shape of predicted distance distributions contains information on the rigidity or flexibility of a protein and its constituent residues.

Supplementary information
Supplementary data are available at Bioinformatics online.
ramr: an R/Bioconductor package for detection of rare aberrantly methylated regions
Authors: Nikolaienko Oleksii , Lønning Per Eystein , Knappskog Stian 
Published: August 2021

AbstractMotivationWith recent advances in the field of epigenetics, the focus is widening from large and frequent disease- or phenotype-related methylation signatures to rare alterations transmitted mitotically or transgenerationally (constitutional epimutations). Merging evidence indicate that such constitutional alterations, albeit occurring at a low mosaic level, may confer risk of disease later in life. Given their inherently low incidence rate and mosaic nature, there is a need for bioinformatic tools specifically designed to analyze such events.ResultsWe have developed a method (ramr) to identify aberrantly methylated DNA regions (AMRs). ramr can be applied to methylation data obtained by array or next-generation sequencing techniques to discover AMRs being associated with elevated risk of cancer as well as other diseases. We assessed accuracy and performance metrics of ramr and confirmed its applicability for analysis of large public datasets. Using ramr we identified aberrantly methylated regions that are known or may potentially be associated with development of colorectal cancer and provided functional annotation of AMRs that arise at early developmental stages.Availability and implementationThe R package is freely available at https://github.com/BBCG/ramr and https://bioconductor.org/packages/ramr.Supplementary informationSupplementary data are available at Bioinformatics online.
Inflection across Categories: Tracking Abstract Morphological Processing in Language Production with MEG
Authors: Hauptman Miriam , Blanco-Elorrieta Esti , Pylkkänen Liina 
Published: August 2021

AbstractCoherent language production requires that speakers adapt words to their grammatical contexts. A fundamental challenge in establishing a functional delineation of this process in the brain is that each linguistic process tends to correlate with numerous others. Our work investigated the neural basis of morphological inflection by measuring magnetoencephalography during the planning of inflected and uninflected utterances that varied across several linguistic dimensions. Results reveal increased activity in the left lateral frontotemporal cortex when inflection is planned, irrespective of phonological specification, syntactic context, or semantic type. Additional findings from univariate and connectivity analyses suggest that the brain distinguishes between different types of inflection. Specifically, planning noun and verb utterances requiring the addition of the suffix -s elicited increased activity in the ventral prefrontal cortex. A broadly distributed effect of syntactic context (verb vs. noun) was also identified. Results from representational similarity analysis indicate that this effect cannot be explained in terms of word meaning. Together, these results 1) offer evidence for a neural representation of abstract inflection that separates from other stimulus properties and 2) challenge theories that emphasize semantic content as a source of verb/noun processing differences.
Sublamina-Specific Dynamics and Ultrastructural Heterogeneity of Layer 6 Excitatory Synaptic Boutons in the Adult Human Temporal Lobe Neocortex
Authors: Schmuhl-Giesen Sandra , Rollenhagen Astrid , Walkenfort Bernd , Yakoubi Rachida , Sätzler Kurt , Miller Dorothea , von Lehe Marec , Hasenberg Mike , Lübke Joachim H R 
Published: August 2021

AbstractSynapses “govern” the computational properties of any given network in the brain. However, their detailed quantitative morphology is still rather unknown, particularly in humans. Quantitative 3D-models of synaptic boutons (SBs) in layer (L)6a and L6b of the temporal lobe neocortex (TLN) were generated from biopsy samples after epilepsy surgery using fine-scale transmission electron microscopy, 3D-volume reconstructions and electron microscopic tomography. Beside the overall geometry of SBs, the size of active zones (AZs) and that of the three pools of synaptic vesicles (SVs) were quantified. SBs in L6 of the TLN were middle-sized (~5 μm2), the majority contained only a single but comparatively large AZ (~0.20 μm2). SBs had a total pool of ~1100 SVs with comparatively large readily releasable (RRP, ~10 SVs L6a), (RRP, ~15 SVs L6b), recycling (RP, ~150 SVs), and resting (~900 SVs) pools. All pools showed a remarkably large variability suggesting a strong modulation of short-term synaptic plasticity. In conclusion, L6 SBs are highly reliable in synaptic transmission within the L6 network in the TLN and may act as “amplifiers,” “integrators” but also as “discriminators” for columnar specific, long-range extracortical and cortico-thalamic signals from the sensory periphery.