bluuebunny/crossref_metadata_2025_split huggingface dataset:
Attitude reconstruction from strap-down rate gyros using power series
Authors: Ghanbarpourasl Habib 
Published: March 2021

AbstractThis paper introduces a power series based method for attitude reconstruction from triad orthogonal strap-down gyros. The method is implemented and validated using quaternions and direction cosine matrix in single and double precision implementation forms. It is supposed that data from gyros are sampled with high frequency and a fitted polynomial is used for an analytical description of the angular velocity vector. The method is compared with the well-known Taylor series approach, and the stability of the coefficients’ norm in higher-order terms for both methods is analysed. It is shown that the norm of quaternions’ derivatives in the Taylor series is bigger than the equivalent terms coefficients in the power series. In the proposed method, more terms can be used in the power series before the saturation of the coefficients and the error of the proposed method is less than that for other methods. The numerical results show that the application of the proposed method with quaternions performs better than other methods. The method is robust with respect to the noise of the sensors and has a low computational load compared with other methods.
Altered Gesture Imitation and Brain Anatomy in Adult Prader–Willi Syndrome Patients
Authors: Caixàs Assumpta , Blanco-Hinojo Laura , Pujol Jesús , Deus Joan , Giménez-Palop Olga , Torrents-Rodas David , Coronas Ramon , Novell Ramon , Esteba-Castillo Susanna 
Published: March 2021

AbstractObjective:To explore motor praxis in adults with Prader–Willi syndrome (PWS) in comparison with a control group of people with intellectual disability (ID) and to examine the relationship with brain structural measurements.Method:Thirty adult participants with PWS and 132 with ID of nongenetic etiology (matched by age, sex, and ID level) were assessed using a comprehensive evaluation of the praxis function, which included pantomime of tool use, imitation of meaningful and meaningless gestures, motor sequencing, and constructional praxis.Results:Results support specific praxis difficulties in PWS, with worse performance in the imitation of motor actions and better performance in constructional praxis than ID peers. Compared with both control groups, PWS showed increased gray matter volume in sensorimotor and subcortical regions. However, we found no obvious association between these alterations and praxis performance. Instead, praxis scores correlated with regional volume measures in distributed apparently normal brain areas.Conclusions:Our findings are consistent in showing significant impairment in gesture imitation abilities in PWS and, otherwise, further indicate that the visuospatial praxis domain is relatively preserved. Praxis disability in PWS was not associated with a specific, focal alteration of brain anatomy. Altered imitation gestures could, therefore, be a consequence of widespread brain dysfunction. However, the specific contribution of key brain structures (e.g., areas containing mirror neurons) should be more finely tested in future research.
Rationale and Design of the National Neuropsychology Network
Authors: Loring David W. , Bauer Russell M. , Cavanagh Lucia , Drane Daniel L. , Enriquez Kristen D. , Reise Steven P. , Shih KuoChung , Umfleet Laura Glass , Wahlstrom Dustin , Whelan Fiona , Widaman Keith F. , Bilder Robert M. ,   for the NNN Study Group
Published: March 2021

AbstractObjective.The National Neuropsychology Network (NNN) is a multicenter clinical research initiative funded by the National Institute of Mental Health (NIMH; R01 MH118514) to facilitate neuropsychology’s transition to contemporary psychometric assessment methods with resultant improvement in test validation and assessment efficiency.Method:The NNN includes four clinical research sites (Emory University; Medical College of Wisconsin; University of California, Los Angeles (UCLA); University of Florida) and Pearson Clinical Assessment. Pearson Q-interactive (Q-i) is used for data capture for Pearson published tests; web-based data capture tools programmed by UCLA, which serves as the Coordinating Center, are employed for remaining measures.Results:NNN is acquiring item-level data from 500–10,000 patients across 47 widely used Neuropsychology (NP) tests and sharing these data via the NIMH Data Archive. Modern psychometric methods (e.g., item response theory) will specify the constructs measured by different tests and determine their positive/negative predictive power regarding diagnostic outcomes and relationships to other clinical, historical, and demographic factors. The Structured History Protocol for NP (SHiP-NP) helps standardize acquisition of relevant history and self-report data.Conclusions:NNN is a proof-of-principle collaboration: by addressing logistical challenges, NNN aims to engage other clinics to create a national and ultimately an international network. The mature NNN will provide mechanisms for data aggregation enabling shared analysis and collaborative research. NNN promises ultimately to enable robust diagnostic inferences about neuropsychological test patterns and to promote the validation of novel adaptive assessment strategies that will be more efficient, more precise, and more sensitive to clinical contexts and individual/cultural differences.
LOCAL COORDINATES FOR COMPLEX AND QUATERNIONIC HYPERBOLIC PAIRS
Authors: GONGOPADHYAY KRISHNENDU , KALANE SAGAR B. 
Published: March 2021

AbstractLet$G(n)={\textrm {Sp}}(n,1)$or${\textrm {SU}}(n,1)$. We classify conjugation orbits of generic pairs of loxodromic elements in$G(n)$. Such pairs, called ‘nonsingular’, were introduced by Gongopadhyay and Parsad for${\textrm {SU}}(3,1)$. We extend this notion and classify$G(n)$-conjugation orbits of such elements in arbitrary dimension. For$n=3$, they give a subspace that can be parametrized using a set of coordinates whose local dimension equals the dimension of the underlying group. We further construct twist-bend parameters to glue such representations and obtain local parametrization for generic representations of the fundamental group of a closed (genus$g \geq 2$) oriented surface into$G(3)$.
NB-IoT devices in reverberation chambers: a comprehensive uncertainty analysis
Authors: Hubrechsen Anouk , Remley Kate A. , Jones Robert D. , Horansky Robert D. , Neylon Vincent T. , Bronckers Laurens A. 
Published: March 2021

AbstractNew protocols related to Internet-of-things applications may introduce previously unnoticed measurement effects in reverberation chambers (RCs) due to the narrowband nature of these protocols. Such technologies also require less loading to meet the coherence-bandwidth conditions, which may lead to higher variations, hence uncertainties, across the channel. In this work, we extend a previous study of uncertainty in NB-IoT and CAT-M1 device measurements in RCs by providing, for the first time, a comprehensive uncertainty analysis of the components related to the reference and DUT measurements. By use of a significance test, we show that certain components of uncertainty become more dominant for such narrowband protocols, and cannot be considered as negligible, as in current standardized test methods. We show that the uncertainty, if not accounted for by using the extended formulation, will be greatly overestimated and could lead to non-compliance to standards.
Long noncoding RNA LINC00518 induces radioresistance by regulating glycolysis through an miR-33a-3p/HIF-1α negative feedback loop in melanoma
Authors: Liu Yan , He Dong , Xiao Mengqing , Zhu Yuxing , Zhou Jianda , Cao Ke 
Published: March 2021

AbstractThe long noncoding RNA, LINC00518, is highly expressed in various types of cancers and is involved in cancer progression. Although LINC00518 promotes the metastasis of cutaneous malignant melanoma (CMM), the mechanism underlaying its effects on CMM radiosensitivity remains unclear. In this study, LINC00518 expression was significantly upregulated in CMM samples, and LINC00518 levels were associated with poor prognosis of patients with CMM. Knockdown of LINC00518 in CMM cells significantly inhibited cell invasion, migration, proliferation, and clonogenicity. LINC00518-mediated invasion, migration, proliferation, and clonogenicity were negatively regulated by the microRNA, miR-33a-3p, in vitro, which increased sensitivity to radiotherapy via inhibition of the hypoxia-inducible factor 1α (HIF-1α)/lactate dehydrogenase A glycolysis axis. Additionally, HIF-1α recognized the miR-33a-3p promoter region and recruited histone deacetylase 2, which decreased the expression of miR-33a-3p and formed an LINC00518/miR-33a-3p/HIF-1α negative feedback loop. Furthermore, signaling with initially activated glycolysis and radioresistance in CMM cells was impaired by Santacruzamate A, a histone deacetylase inhibitor, and 2-deoxy-D-glucose, a glycolytic inhibitor. Lastly, knockdown of LINC00518 expression sensitized CMM cancer cells to radiotherapy in an in vivo subcutaneously implanted tumor model. In conclusion, LINC00518 was confirmed to be an oncogene in CMM, which induces radioresistance by regulating glycolysis through an miR-33a-3p/HIF-1α negative feedback loop. Our study, may provide a potential strategy to improve the treatment outcome of radiotherapy in CMM.
Inhibiting β-catenin disables nucleolar functions in triple-negative breast cancer
Authors: Weeks Shannon E. , Kammerud Sarah C. , Metge Brandon J. , AlSheikh Heba A. , Schneider David A. , Chen Dongquan , Wei Shi , Mobley James A. , Ojesina Akinyemi I. , Shevde Lalita A. , Samant Rajeev S. 
Published: March 2021

AbstractTriple-negative breast cancer (TNBC) patients with upregulated Wnt/β-catenin signaling often have poor clinical prognoses. During pathological examinations of breast cancer sections stained for β-catenin, we made the serendipitous observation that relative to non-TNBC, specimens from TNBC patients have a greater abundance of nucleoli. There was a remarkable direct relationship between nuclear β-catenin and greater numbers of nucleoli in TNBC tissues. These surprising observations spurred our investigations to decipher the differential functional relevance of the nucleolus in TNBC versus non-TNBC cells. Comparative nucleolar proteomics revealed that the majority of the nucleolar proteins in TNBC cells were potential targets of β-catenin signaling. Next, we undertook an analysis of the nucleolar proteome in TNBC cells in response to β-catenin inhibition. This effort revealed that a vital component of pre-rRNA processing, LAS1 like ribosome biogenesis factor (LAS1L) was significantly decreased in the nucleoli of β-catenin inhibited TNBC cells. Here we demonstrate that LAS1L protein expression is significantly elevated in TNBC patients, and it functionally is important for mammary tumor growth in xenograft models and enables invasive attributes. Our observations highlight a novel function for β-catenin in orchestrating nucleolar activity in TNBCs.
Small intestinal immune-environmental changes induced by oral tolerance inhibit experimental atopic dermatitis
Authors: Um Han-Na , Baek Jin-Ok , Park Sohyeon , Lee Eun-Hui , Jang Jinsun , Park Woo-Jae , Roh Joo-Young , Jung YunJae 
Published: March 2021

AbstractAtopic dermatitis is a chronic skin inflammatory disease mediated by Th2-type immune responses. Although intestinal immune responses have been shown to play a critical role in the development or prevention of atopic dermatitis, the precise influence of intestinal immunity on atopic dermatitis is incompletely understood. We show here that orally tolerized mice are protected from experimental atopic dermatitis induced by sensitization and epicutaneous (EC) challenge to ovalbumin. Although the expression of Th2-type cytokines in the small intestine of orally tolerized and EC-challenged mice did not change significantly, these mice showed decreased inflammatory responses in the small intestine with restoration of microbial change elicited by the EC challenge. Interestingly, an increase in small intestinal eosinophils was observed with the EC challenge, which was also inhibited by oral tolerance. The role of small intestinal eosinophils and microbiota in the pathogenesis of experimental atopic dermatitis was further substantiated by decreased inflammatory mediators in the small intestine and attenuated Th2-type inflammation in the skin of eosinophil-deficient and microbiota-ablated mice with EC challenges. Based on these data, we propose that the bidirectional interaction between the skin and the intestine has a role in the pathogenesis of atopic dermatitis and that modulation of the intestinal microenvironments could be a therapeutic approach to atopic dermatitis.
Enzymatic spiroketal formation via oxidative rearrangement of pentangular polyketides
Authors: Frensch Britta , Lechtenberg Thorsten , Kather Michel , Yunt Zeynep , Betschart Martin , Kammerer Bernd , Lüdeke Steffen , Müller Michael , Piel Jörn , Teufel Robin 
Published: March 2021

AbstractThe structural complexity and bioactivity of natural products often depend on enzymatic redox tailoring steps. This is exemplified by the generation of the bisbenzannulated [5,6]-spiroketal pharmacophore in the bacterial rubromycin family of aromatic polyketides, which exhibit a wide array of bioactivities such as the inhibition of HIV reverse transcriptase or DNA helicase. Here we elucidate the complex flavoenzyme-driven formation of the rubromycin pharmacophore that is markedly distinct from conventional (bio)synthetic strategies for spiroketal formation. Accordingly, a polycyclic aromatic precursor undergoes extensive enzymatic oxidative rearrangement catalyzed by two flavoprotein monooxygenases and a flavoprotein oxidase that ultimately results in a drastic distortion of the carbon skeleton. The one-pot in vitro reconstitution of the key enzymatic steps as well as the comprehensive characterization of reactive intermediates allow to unravel the intricate underlying reactions, during which four carbon-carbon bonds are broken and two CO2 become eliminated. This work provides detailed insight into perplexing redox tailoring enzymology that sets the stage for the (chemo)enzymatic production and bioengineering of bioactive spiroketal-containing polyketides.
Ultrastable liquid crystalline blue phase from molecular synergistic self-assembly
Authors: Hu Wei , Wang Ling , Wang Meng , Zhong Tingjun , Wang Qian , Zhang Lanying , Chen Feiwu , Li Kexuan , Miao Zongcheng , Yang Dengke , Yang Huai 
Published: March 2021

AbstractFabricating functional materials via molecular self-assembly is a promising approach, and precisely controlling the molecular building blocks of nanostructures in the self-assembly process is an essential and challenging task. Blue phase liquid crystals are fascinating self-assembled three-dimensional nanomaterials because of their potential information displays and tuneable photonic applications. However, one of the main obstacles to their applications is their narrow temperature range of a few degrees centigrade, although many prior studies have broadened it to tens via molecular design. In this work, a series of tailored uniaxial rodlike mesogens disfavouring the formation of blue phases are introduced into a blue phase system comprising biaxial dimeric mesogens, a blue phase is observed continuously over a temperature range of 280 °C, and the range remains over 132.0 °C after excluding the frozen glassy state. The findings show that the molecular synergistic self-assembly behavior of biaxial and uniaxial mesogens may play a crucial role in achieving the ultrastable three-dimensional nanostructure of blue phases.
Transcriptome and chromatin landscape of iNKT cells are shaped by subset differentiation and antigen exposure
Authors: Murray Mallory Paynich , Engel Isaac , Seumois Grégory , Herrera-De la Mata Sara , Rosales Sandy Lucette , Sethi Ashu , Logandha Ramamoorthy Premlal Ashmitaa , Seo Goo-Young , Greenbaum Jason , Vijayanand Pandurangan , Scott-Browne James P. , Kronenberg Mitchell 
Published: March 2021

AbstractInvariant natural killer T cells (iNKT cells) differentiate into thymic and peripheral NKT1, NKT2 and NKT17 subsets. Here we use RNA-seq and ATAC-seq analyses and show iNKT subsets are similar, regardless of tissue location. Lung iNKT cell subsets possess the most distinct location-specific features, shared with other innate lymphocytes in the lung, possibly consistent with increased activation. Following antigenic stimulation, iNKT cells undergo chromatin and transcriptional changes delineating two populations: one similar to follicular helper T cells and the other NK or effector like. Phenotypic analysis indicates these changes are observed long-term, suggesting that iNKT cells gene programs are not fixed, but they are capable of chromatin remodeling after antigen to give rise to additional subsets.
The methyltransferase SETD2 couples transcription and splicing by engaging mRNA processing factors through its SHI domain
Authors: Bhattacharya Saikat , Levy Michaella J. , Zhang Ning , Li Hua , Florens Laurence , Washburn Michael P. , Workman Jerry L. 
Published: March 2021

AbstractHeterogeneous ribonucleoproteins (hnRNPs) are RNA binding molecules that are involved in key processes such as RNA splicing and transcription. One such hnRNP protein, hnRNP L, regulates alternative splicing (AS) by binding to pre-mRNA transcripts. However, it is unclear what factors contribute to hnRNP L-regulated AS events. Using proteomic approaches, we identified several key factors that co-purify with hnRNP L. We demonstrate that one such factor, the histone methyltransferase SETD2, specifically interacts with hnRNP L in vitro and in vivo. This interaction occurs through a previously uncharacterized domain in SETD2, the SETD2-hnRNP Interaction (SHI) domain, the deletion of which, leads to a reduced H3K36me3 deposition. Functionally, SETD2 regulates a subset of hnRNP L-targeted AS events. Our findings demonstrate that SETD2, by interacting with Pol II as well as hnRNP L, can mediate the crosstalk between the transcription and the splicing machinery.
Real-time determination of earthquake focal mechanism via deep learning
Authors: Kuang Wenhuan , Yuan Congcong , Zhang Jie 
Published: March 2021

AbstractAn immediate report of the source focal mechanism with full automation after a destructive earthquake is crucial for timely characterizing the faulting geometry, evaluating the stress perturbation, and assessing the aftershock patterns. Advanced technologies such as Artificial Intelligence (AI) has been introduced to solve various problems in real-time seismology, but the real-time source focal mechanism is still a challenge. Here we propose a novel deep learning method namely Focal Mechanism Network (FMNet) to address this problem. The FMNet trained with 787,320 synthetic samples successfully estimates the focal mechanisms of four 2019 Ridgecrest earthquakes with magnitude larger than Mw 5.4. The network learns the global waveform characteristics from theoretical data, thereby allowing the extensive applications of the proposed method to regions of potential seismic hazards with or without historical earthquake data. After receiving data, the network takes less than two hundred milliseconds for predicting the source focal mechanism reliably on a single CPU.
Catalytic activity tunable ceria nanoparticles prevent chemotherapy-induced acute kidney injury without interference with chemotherapeutics
Authors: Weng Qinjie , Sun Heng , Fang Chunyan , Xia Fan , Liao Hongwei , Lee Jiyoung , Wang Jincheng , Xie An , Ren Jiafeng , Guo Xia , Li Fangyuan , Yang Bo , Ling Daishun 
Published: March 2021

AbstractAcute kidney injury (AKI) is a prevalent and lethal adverse event that severely affects cancer patients receiving chemotherapy. It is correlated with the collateral damage to renal cells caused by reactive oxygen species (ROS). Currently, ROS management is a practical strategy that can reduce the risk of chemotherapy-related AKI, but at the cost of chemotherapeutic efficacy. Herein, we report catalytic activity tunable ceria nanoparticles (CNPs) that can prevent chemotherapy-induced AKI without interference with chemotherapeutic agents. Specifically, in the renal cortex, CNPs exhibit catalytic activity that decomposes hydrogen peroxide, and subsequently regulate the ROS-involved genes by activating the Nrf2/Keap1 signaling pathway. These restore the redox homeostasis for the protection of kidney tubules. Under an acidic tumor microenvironment, CNPs become inert due to the excessive H+ that disrupts the re-exposure of active catalytic sites, allowing a buildup of chemotherapy-mediated ROS generation to kill cancer cells. As ROS-modulating agents, CNPs incorporated with context-dependent catalytic activity, hold a great potential for clinical prevention and treatment of AKI in cancer patients.
Biodiversity of non-Saccharomyces yeasts associated with spontaneous fermentation of Cabernet Sauvignon wines from Shangri-La wine region, China
Authors: Zhao Yue , Sun Qingyang , Zhu Shusheng , Du Fei , Mao Ruzhi , Liu Lijing , Tian Bin , Zhu Yifan 
Published: March 2021

AbstractShangri-La is a wine region that has the highest altitude vineyards in China. This is the first study investigated the biodiversity of non-Saccharomyces yeasts associated with spontaneous fermentation of Cabernet Sauvignon wines produced from two sub-regions (Lancang River and Jinsha River) of Shangri-La. The culturable yeasts were preliminarily classified based on their colonial morphology on the Wallerstein Laboratory nutrient agar plates. Yeast species were identified by the sequencing of the 26S rRNA D1/D2 region and the 5.8S rRNA ITS region. Twenty-five non-Saccharomyces yeast species belonging to sixteen genera were isolated and identified in Shangri-La wine region. Candida, Hanseniaspora, Pichia, and Starmerella were found in both sub-regions, but the Lancang River showed more diverse yeast species than the Jinsha River. Shangri-La not only exhibited high diversity of non-Saccharomyces yeasts, and furthermore, seven species of non-Saccharomyces yeasts were exclusively found in this region, including B. bruxellensis, D. hansenii, M. guilliermondii, S. vini, S. diversa, T. delbrueckii and W. anomalus, which might play an important role in distinctive regional wine characteristics. This study provide a relatively comprehensive analysis of indigenous non-Saccharomyces yeasts associated with Cabernet Sauvignon from Shangri-La, and has significance for exploring ‘microbial terroir’ of wine regions in China.