bluuebunny/crossref_metadata_2025_split huggingface dataset:
Genetic characterization of iler (Plectranthus scutellarioides (L.) R. Br.) based on RAPD molecular marker
Authors: Subositi D , Rosdiana D , Yuniaty A , Susanti D , Maruzy A , Rahmawati N W 
Published: January 2021

Abstract
Iler (Plectranthus scutellarioides (L.) R. Br.) is an ornamental plant and also used as medicinal plant. The plant is one of medicinal plant for anti hemorrhoids Jamu Saintifik herbal formula in B2P2TOOT Indonesian Ministry of Health. This will lead to commercialization opportunities in herbal drug industry that recently has become increasingly popular. With the emergence of substitution and adulteration of herbal drug and the lack of information on coleus genetic diversity which may result in misidentification, this research was conducted to determine Iler genetic characteristics using RAPD markers. The genomic DNA was extracted from 15 accessions of iler and 3 accessions from genus Plectranthus as an outgroup. Six selected RAPD primers were used in amplification. Dice similarity index was used to calculate similarity index followed by cluster analysis and dendrogram contruction using Unweighted Pair Group Method with Arithmetic Mean (UPGMA). The results showed 52 DNA fragments were produce within the size range 293-2,667 bp and polymorphism level among accessions at 94.23%. Cluster results results in genetic diversity ranging from 40.45-97.87% and a dendogram that shows clustering trend of Iler accessions based on the similarity of morphological characters such as leaf shape and color.
Application of biological control Paenibacillus polymyxa toward bacterial leaf blight disease in rice plant
Authors: Mumpuni R P , Rohmah A J 
Published: January 2021

Abstract
Diseases that often attack rice plants are bacterial leaf blight, this disease can reduce rice production by up to 60%. Control of bacterial leaf blight in addition to using chemicals can also use biological agents, one of which is Paenibacillus polymyxa. Testing of biological control applications begins with inoculation of Xanthomonas oryzae bacteria on ciherang rice seeds 9 days after planting (DAP). Planting rice using Randomized Block Design using biological control Paenibacillus polymyxa consists of four treatments, Control (P0), 2,5 ml l-1 (P1), 5 ml l-1 (P2), and 7,5 ml l-1 (P3). The biological control application is carried out on seeds, 10 DAP, 2 week after planting (WAP), and 4 WAP. Observations were made once a week to observe the rice growth and intensity of the disease. Observations were made in the vegetative phase. The results of the Paenibacillus polymyxa biological control test can reduce the level of bacterial leaf blight attack even though the difference in each treatment is not significantly different. The recommended concentration of Paenibacillus polymyxa biological control is 7,5 ml l-1. This concentration can reduce the level of bacterial leaf blight attack in the vegetative phase.
Cashew : Contribution to the household economy and carbon stock (Case study in Wonogiri regency)
Authors: Pujiasmanto B , Rahayu E S , Murniyanto E 
Published: January 2021

AbstractWonogiri Regency is a cashew crop center in Central Java. Cashew is planting in dryland at an elevation of 100 to 400 m above sea level with a monoculture and multicultural pattern. Cashew plants are cultivated as people plantations, planted regularly on each plot or as a fence on the ownership limits of dry land, plots, and mounds. Cashew plants are evergreen, have a tight canopy with sympodial branching patterns, flowering all the time so they can cover the surface of the soil. Such plant patterns provide acceptance for households and protect the environment. Research to reveal the contribution of household income, land cover rates, and carbon stocks has been carried out by survey methods. Data collection techniques were carried out by interviewing the head of the household and the measurement of cashew land. The results showed that the B / C ratio > 1 and increased with the age of the plant, it contributed to the acceptance of household income. Canopy cover and carbon stock increase every year so that it is beneficial to maintain soil fertility.
The phytoextraction of Cu and Zn by elephant grass (Pennisetum purpureum) from tropical soil 21 years after amendment with industrial waste containing heavy metals
Authors: Salam A K , Hidayatullah M A , Supriatin S , Yusnaini S 
Published: January 2021

Abstract
Increased soil heavy metal concentrations are suggested to cause roots to work harder. This research was to study the growth and phytoextraction behaviors of elephant grass in soil amended with industrial waste. Soil samples were obtained from an experimental field treated with a heavy metal containing waste at 0, 15 and 60 Mg ha-1, CaCO3 at 0 and 5 Mg ha-1, and compost at 0 and 5 Mg ha-1. Soil samples were planted with elephant grass, 8 weeks after which the soil samples were analyzed for Cu and Zn. Plant roots and shoots were harvested and weighed for their dry-masses and analyzed for Cu and Zn. The results demonstrate that the Root/Shoot increased and show good correlations with the increase in soil Cu or Zn. The plant Cu or Zn increased with the increase in soil Cu or Zn but decreased with liming. Plant Cu and Zn in roots and the whole plants as well as their TFs were well correlated with soil Cu and Zn. These observations confirm that the root/shoot growth and Cu and Zn absorption by elephant grass are governed by soil Cu and Zn and elephant grass is a Cu and Zn phytoextractor.
Response of Artemisia annua accessions to nitrogen fertilizer on low land
Authors: Widiyastuti Y , Subositi D , Haryanti S 
Published: January 2021

Abstract
Growing Artemisia annua in tropical areas cause a short vegetative period and resulted in low content of artemisinin. To increase the artemisinin content of A. annua in the tropical area could be carried out by administering of Nitrogen fertilizer. In order to increase production and extension of grow areas, the selection of superior accession which adaptable in low areas is also needed. The study was carried out using an experimental method by applying 3 levels dosage of Nitrogen fertilization (0,100, 200 and 300 kg/Ha) to 3 A. annua accessions (V1, V2 and V3). The research was conducted at April-August 2018 at Karangpandan research station with an elevation of 600 m asl. The parameters observed were plant height, stem diameter, number of branches, fresh biomass weight, dry biomass weight, artemisinin content and essential oil content. The results showed that the growth and productivity of 3 accession of A. annua is strongly influenced by the Nitrogen dosage. The lower dosage of Nitrogen decline growth and productivity, and plant entry into the generative phase later for the highest dosage of Nitrogen. The Nitrogen application didn’t effect on the artemisinin as well as the volatile oil content of A. annua.
Antibacterial effects of Kepok Banana bunch (Musa paradisiaca L.) against Staphylococcus aureus
Authors: Maryati T , Nugroho T , Bachruddin Z , Pertiwiningrum A 
Published: January 2021

Abstract
Banana plants are well-known to contain diverse phytochemical compound. This study aimed to identify the antibacterial effects of Kepok Banana bunches (Musa paradisiaca L.) against Staphylococcus aureus bacteria. A laboratory analysis was carried out to determine the zone of bacterial inhibition. The experiment used completely randomized design with 3 treatments (50 μL, 70 μL Kepok Banana bunches extract and 50 μL Chloramphenicol as a positive control), each treatment replicated three times. The study was performed used wells agar diffusion. Data were analyzed by analysis of variance (ANOVA) and followed by Duncan Multiple Range Test (DMRT). Results showed that Kepok Banana bunches had potency as antibacterial agent. Treatments showed different (P<0.05) results in bacterial inhibition zones. Chloramphenicol produces the highest inhibitory zone (34.78 mm), while the difference in the concentration of banana bunches produces the same inhibitory zone (50 μL: 11.52 mm; 70 μL: 15.52). It is concluded that Kepok Banana bunches has potency to use as an antibacterial against Staphylococcus aureus.
The effect of sludge dairy cattle and expired milk powder waste as growth media for white oyster mushroom (Pleurotus florida)
Authors: Sirajuddin M M , Muhlisin  , Pertiwiningrum A 
Published: January 2021

Abstract
White oyster mushroom media must add additional nutrients as stimulants for mushroom growth like bran, but bran is cattle feed so its availability is limited. Sludge biogas of cattle and expired milk powder waste is the result of livestock waste which is still rich in nutrients which are important substances. This study aims to determine the effect of differences in the composition of the biogas sludge media and expired milk powder waste on mushroom productivity. This study was designed with four treatments and three repetitions which difference in the ratio of the concentration of cattle biogas sludge and expired milk powder with a ratio of P0 (0g: 0g), P1 (100g: 100g), P2 (50g: 150g) P3: (150g: 50g). Data taken include mushroom growth measurement The data was processed using analysis of Variance-Completely Randomized Design Unidirectional and the average difference was tested using Duncan’s Multiple Range Test (DMRT). The best result is P2 treatment, fresh weight, a diameter of caps, stem length, and harvest time obtained the results are 124.15 gram, 10.57 cm, 5.98 cm, and 46.00 days. In this study, the greater the proportion of expired milk powder waste that was given increasingly increased the growth of white oyster mushrooms.
Analysis of mangrove management to increase the economy of coastal communities in Madura island
Authors: Wahyurini E T , Darsono  , Kusnandar  , Harisudin M 
Published: January 2021

Abstract
The purpose of the research was to analyze the effect of mangrove forest management on the economic development of the coastal community on Madura Island. This paper outlines the management of mangrove forests for economic development on Madura Island. The research method used quantitative and qualitative descriptive-analytic used quantitative data. Data collected by interview, observation, and literature study. Respondents in this study came from four districts in Madura, namely Bangkalan, Sampang, Pamekasan and Sumenep, which consisted of water management communities, Related Offices, Environmentalists, Academics, NGOs / Institutions, and CSR totaling 170 respondents. Data analysis tool was done by SEM Warp Partial Least Square method. The results show that mangrove forest management has a significant positive effect on economic development. Improving economic development, in this case, was increasing income, new employment opportunities, and mangrove sustainability.
Does RSPO certification affects the amount of CO2 emission in Indonesia?
Authors: Hilmi Y S , Utami A W 
Published: January 2021

Abstract
As the most giant Crude Palm Oil (CPO) producer, Indonesia faces environmental issues. In 2004, the adoption of Roundtable Sustainable Palm Oil (RSPO) aimed at reducing the negative effect created by the palm oil industries. This study examined the impact of RSPO certification in the Indonesian palm oil industries on the amount of CO2 as one component of GHG emissions. This study used time series data from 1981 to 2016, collected from the World Bank, the Tree Crop Estate Statistics of Indonesia, and UnComtrade. Vector Error Correction Model (VECM), with the amount of CO2 emissions in Indonesia as the dependent variables, was run against the area used to produce CPO, the amount of CPO produced by Indonesia, the CPO price of Indonesia, and the dummy variable that is RSPO certification. The results show that the model has both short and long-term equilibrium relationships. While the RSPO certification as a dummy variable is not associated with the amount of CO2 emissions in Indonesia.
Germination and seedling growth of Kipas Putih soybean (Glycine max [L.] Merril) in various dosage of gamma rays irradiation
Authors: Nilahayati  , Hanafiah D S , Rosmaina  
Published: January 2021

Abstract
Gamma irradiation is the widely used mutagen in assembling plant genetic diversity. The important thing to do is looking for the most appropriate dose that gives rise of diversity without turning off irradiated plants. This study aims to determine the initial growth response of Kipas Putih soybean seeds due to gamma ray irradiation treatment. Kipas Putih soybean seeds measure their water content up to 11%. Furthermore, gamma ray irradiation was carried out at doses 0, 200, 400, 600, 800 and 1000 Gray. The results showed that there was decrease pe rcentage of germination in Kipas Putih soybean seeds at 200 Gray reaching 50% at 14th day after planting. Irradiation doses of 400 Gray and 600 Gray reduce percentage of germination until 20% and 30%, respectively. While at doses of 800 Gray and 1000 Gray, all seeds died at 21st day after planting. Giving gamma ray irradiation also showed a decrease in seedling height along with increasing irradiation dose.
Effect of Benzilaminopurine and Kinetin for shoot multiplication of Indigofera (Indigofera zollingeriana Miq.) by in vitro culture
Authors: Royani J I , Chotimah S , Utami R N , Fatma W S , Susiyanti  , Fatmawaty A P 
Published: January 2021

Abstract
Indigofera (Indigofera zollingeriana Miq.) is one of legume trees that useful as forage and have advantages in production and quality of forage compared to other types of legumes. This forage contains high protein and mineral sources, good fiber structure and high digestibility value. Nutrient contents are crude protein (31%) with 76% of DMD and 83% of OMD. Indigofera has low germination due to thick seed coat and fungal invasion during germination. Propagation by in vitro culture promise to multiply superior seeds from Indigofera. The aim for this research was to determine the effect of Benzylaminopurine (BAP) and Kinetin for multiply of shoots of Indigofera. The research was designed using Completely Randomized Design (CRD) with 2 factors (BAP and Kinetin concentration) and repeated 3 times. BAP concentration consists of four levels, 0, 1.0, 1.5 and 2 mg/L and Kinetin concentration consists of four levels 0, 1.0, 2 and 3 mg/L. Results showed that BAP gave the best results for number of shoots parameter at any concentration. Compact callus appears in basal of shoots at BAP (1.5 mg/L and 2 mg/L) media. Whereas media with Kinetin showed no significant effect on all parameters and there was no interaction between BAP and Kinetin.
Consumer perception on selecting marketplace for livestock products food
Authors: Muzayyanah M A U , Putra A R S , Syahlani S P , Dewi N H U 
Published: January 2021

Abstract
The purpose of this paper is to analyses the relationship between the underlying factors of product and market attributes and socio-demographic profiles of consumers. The aim of the analysis is to assess whether consumer responses vary across product and market attributes. Simple statistical analysis such as descriptive statistical analysis, frequency distribution, cross tabulation, analysis of variance, and factor analysis to assess the consumers’ preferences for livestock products food and market attributes were carried out. Results of this study indicate that indicate that the socio-demographic profile of consumers (gender, age, education and income) significantly influences the purchase decisions. A higher income gender and educational level of consumers influences their decisions on product and market attributes while age seems to have no significant impact. Consumers express significantly different views on various product attributes. Packaging and convenience are important for approximately 60 per cent respondents. Various market attributes clearly indicate that consumers prefer a convenient marketplace with additional service facilities. Development of marketing strategy regarding the products that can be offered at a marketplace based on consumer preferences and behavior.
Carbon stock estimation on some land cover: secondary forest, agroforestry, palm oil plantation and paddy fields
Authors: Karuru S S , Rasyid B , Millang S 
Published: January 2021

Abstract
The diversity of plant in some land uses at East Luwu Regency has potential to absorb and store carbon, which varies due to the plant different constituent. The differences of carbon stored of each land cover need to calculate in order to find the amount of carbon stock available. This study aims to estimate carbon stocks in the land cover of secondary forest, agroforestry, oil palm plantation and rice fields. The research use sample plot size 20 × 50 that taken 9 times for each land cover. Biomass data was collected using non-destructive sampling, since for undergrowth and necromass using destructive sampling by cutting and taking all the undergrowth and litter that are in a 1 × 1 meter quadrant. There are two kinds of soil sampling that collected; disturbed soil and intact soil. The results showed that the highest estimated carbon stock is secondary forest with carbon stock value of 265.86 tons / ha, as for agroforestry the carbon stock value of 131.31 tons / ha, while oil palm with a carbon stock value of 100.89 tons / ha and the lowest is paddy fields with carbon stock value of 70.50 tons / ha.
The effects of catfish oil supplementation as unsaturated fatty acid source on Bali cow gas production kinetics, dry matter digestibility, and organic matter digestibility in vitro
Authors: Cahyo D N , Yusiati L M , Kurniawati A , Hanim C , Muhlisin  
Published: January 2021

AbstractThe purpose of this study was to investigate the effects of catfish oil (CFO) supplementation on gas production kinetics, dry matter (IVDMD), and organic matter digestibility in vitro (IVOMD) with Bali cow rumen fluid. The design of this study was a completely randomized design with 5 treatment consist of T0 (control diet:Pennisetum purpupoides(60): wheat pollard (30): soybean meal (10)), T1 (T0 + 2% DM CFO), T2 (T0 + 4% DM CFO), T3 (T0 + 6% DM CFO), T4 (T0 + 8% DM CFO) and 3 replication. Gas production technique described by Menke and Steingass was used in this study with 48 hours of incubation time. Gas production kinetics was analyzed by Fit Curve application. The result showed that CFO supplementation did not change the value of gas production, a fraction value, b fraction value, gas production rate (c value), IVDMD, and IVOMD, but the increasing supplementation at the level of 8% decreased (P<0.05) gas production, b value, IVDMD, and IVOMD. It could be concluded that catfish oil supplementation as unsaturated fatty acid source at the level of 6% DM in the Bali cow diet did not give a negative effect on rumen substrates degradation.
Economic feasibility analysis of service business of agricultural equipment and machinery in Kubu Raya Regency, Indonesia
Authors: Ekawati  , Ellyta  , Sugiardi S 
Published: January 2021

Abstract
Agricultural equipment and machinery have become the main farmer’s need as technically it can accelerate and improve the quality of soil cultivation, water supply, increase the cropping index, reduce lost yields and preserve the environmental functions. Economically, it can increase the added values through product processing, productivity, and resource efficiency. Institutional service business of agricultural equipment and machinery (UPJA) needs to develop to facilitate farmers’ access to the use of affordable agricultural equipment and machinery in order to increase the sustainable food production. The sustainability of the service business is influenced by many aspects including economic aspects. This study aimed to analyze financial feasibility of UPJA in Parit Keladi village, Sungai Kakap subdistrict, Kubu Raya Regency. Analysis of R/C ratio and B/C ratio was applied. Based on R/C ratio of feasibility analysis, all kinds of agricultural equipment and machinery including transplanter, water pump, rice thresher, mini combine harvester, and hand tractor provided by the service business were feasible, shown by R/C ratio> 1.52 and B/C ratio >0.52. Whereas, The R/C value of water pump of 2.5; hand tractor 2.0; 1.81 transplant machine and 1.67 mini combaine and 1.31 power thresher machine, respectively.
Evaluation of growth and biomass production of sorghum on cadmium contaminated paddy field
Authors: Hasanah N A U , Harsono P , Samanhudi  , Purwanto E 
Published: January 2021

AbstractThe use of agrochemicals that exceed doses and over-exploitation of land for an agricultural commodity contributes to increasing cadmium concentration. Sorghum is a multifunctional crop that can be potential as a heavy metal remediation plant. The study aimed to obtain potential sorghum varieties for phytoremediation of cadmium by assessing the growth and capacity of sorghum biomass in Cd contaminated paddy fields. The study was arranged in a complete randomized factor group with four replications. Six sorghum varieties tested were: Super 1, Samurai, Suri 3, Numbu, Kawali, and Hitam. These varieties were planted with cadmium contaminated rice fields. In general, sorghum varieties can grow in cadmium contaminated rice fields. Three of the six varieties tested namely Super 1, Samurai, and Kawali have advantages in growth such as plant height, leaf area, leaf chlorophyll content, plant growth rate, and net assimilation rate. Big biomass performance is also approved by these three varieties. The results in this study will be described and used as a reference to complement the ability of this variety in the absorption and accumulation ability.
Identification of antagonistic bacteria against peanut stem rot disease (Sclerotium rolfsii Sacc.) on the peatland of Kuala Pesisir-Nagan Raya, Indonesia
Authors: Subandar I , Hakim L , Suliansyah I , Syakur S 
Published: January 2021

Abstract
Peanut cultivation in peatlands has major obstacles in terms of controlling plant diseases. This research aimed to identify and characterize bacteria which have the potential as biological control agents against S. rolfsii in the peatlands of Kuala Pesisir Nagan Raya. This research was conducted in the Kuala Pesisir sub-district, Nagan Raya District, Aceh, Indonesia. The research activities include isolation, morphological identification, pathogenicity testing, antagonistic testing, and molecular identification. After the first screening, 25 bacterial isolates were to be observed further. At the root of the peanut plants the colonies were mostly bacillus, some of them were coccus. In gram staining, there were many gram-negative isolates compared to gram positive. Non-pathogenic bacterial isolates have good growth potential (85% - 100%), except for B3 bacterial isolates (74%). The peanut seeds in non-pathogenic bacteria that have good germination were in the range of 80% - 96% of the germination capacity. Peatland bacteria that interact with type D have the potential to act as antagonistic agents against S. rolfsii. There are bacteria which were promising to act as an antagonistic agent against Sclerotium rolfsii in the research area. Based on morphological and DNA characteristics, the bacteria were identified as Bacillus sp.