bluuebunny/crossref_metadata_2025_split huggingface dataset:
Intermittent theta burst stimulation for negative symptoms in schizophrenia patients with mild cognitive impairment: a randomized controlled trail
Authors: Li Jing , Mo Xian , Jiang Dan , Huang Xinyu , Wang Xiao , Xia Tingting , Zhang Wei 
Published: January 2025

BackgroundThis study aims to evaluate the intervention effect of intermittent Theta burst stimulation (iTBS) on bilateral dorsomedial prefrontal cortex (DMPFC) for negative symptoms in schizophrenia using functional near-infrared spectroscopy (fNIRS) to confirm the therapeutic significance of DMPFC in treating negative symptoms and provide new evidence for schizophrenia treatment and research.MethodThirty-nine schizophrenia patients with negative symptoms and mild cognitive impairment were randomly divided into a treatment group (n=20) and a control group (n=19). The treatment group received iTBS in bilateral DMPFC. The control group received the sham treatment. Negative symptoms, cognitive function, emotional state, and social function were assessed at pre-treatment, post-treatment, 4-, 8-, and 12-week follow-ups. Brain activation in regions of interest (ROIs) was evaluated through verbal fluency tasks. Changes in scale scores were analyzed by repeated measures ANOVA.ResultAfter 20 sessions of iTBS, the Scale for the Assessment of Negative Symptoms (SANS) total and sub-scale scores significantly improved in the treatment group, with statistically significant differences. SANS scores differed significantly between pre- and post-treatment in both groups, with post-treatment scores markedly lower than pre-treatment and better efficacy in the treatment group. However, there was no significant difference in cognitive function, emotional state, and social function. ROIs did not differ significantly between groups before intervention. After treatment, prefrontal cortex activation was significantly higher in the treatment group than in controls, with a statistically significant difference. Regarding functional connectivity, the small-world properties Sigma and Gamma were enhanced.ConclusioniTBS on bilateral DMPFC can effectively alleviate negative symptoms and enhance prefrontal cortex activation and the small-world properties in patients of schizophrenia.
Sex differences in the association between metabolic disorder and inflammatory cytokines in Han Chinese patients with chronic schizophrenia
Authors: Tian Yinghan , Li Zhenkuo , Zhang Yun , Tang Pei , Zhuang Yu , Liu Lewei , Fan Haojie , Yao Xianhu , Li Wenzheng , Xia Lei , Liu Huanzhong 
Published: January 2025

BackgroundPrevious evidence suggests that immune-inflammatory dysfunction plays an important role in metabolic disorder (MD) of patients with schizophrenia, yet studies on sex differences in the association between both are limited. The current study aimed to investigate sex differences in the association between MD and inflammatory cytokines in Han Chinese patients with chronic schizophrenia (CS).MethodsThis multicentre cross-sectional study was conducted in one general hospital and two psychiatric hospitals in Anhui Province, China. General information, metabolic parameters and inflammatory cytokine indicators were collected from all participants, and the severity of their psychiatric symptoms was assessed using the Positive and Negative Syndrome Scale (PANSS).ResultsA total of 324 patients completed the assessment and were included in this study. The patients with MD had higher age and duration of illness, and lower chlorpromazine equivalents and negative symptom score than those without MD. Binary logistic regression showed that MD was significantly associated with a higher IL-1β level in male patients, while this association was not significant in females. Further multiple linear regression revealed that IL-1β level was negatively correlated with polypharmacy only in male patients.ConclusionSex differences in the association between MD and IL-1β level are significant in patients with CS, and only in male patients is there a negative correlation between MD and IL-1β level. Sex-specific prevention and intervention strategies may contribute to reducing MD in patients with CS.
Green space is associated with new-onset stroke among Chinese middle-aged and older adults: data from China Health and Retirement Longitudinal Study (CHARLS)
Authors: Lu Qiong , Lian Cheng , Chen Xinglin 
Published: January 2025

Background and aimsThe relationship between green space and new-onset stroke is inconclusive. This study aimed to investigate the association between green space and stroke risk among Chinese middle-aged and older adults.MethodsData were taken from participants aged ≥45 years in the China Health and Retirement Longitudinal Study (CHARLS). Baseline data were collected in 2011 and new-onset stroke data were gathered during follow-up in 2013, 2015, 2018, and 2020. Multivariate Cox regression models were constructed to investigate the association between green space and stroke risk. Subgroup analysis was also performed.ResultsA total of 13,696 participants with a mean age of 59.3 ± 9.3 years were included. After a mean follow-up duration of 6.32 years, there were 728 stroke events during a total of 86,530 person-years of follow-up. The study found a L-shaped relationship between green space and the risk of new-onset stroke in participants. By using a two-piecewise linear regression model, we calculated that the inflection point for the per capita park green area was 10.61 square meters per person (log-likelihood ratio test p = 0.041). On the left of the inflection point, we observed a negative relationship between green space and the incidence of stroke (HR: 0.89, 95% CI: 0.84–0.94, p = 0.0001). On the right side of the inflection point, however, the relationship tended to be saturated (HR: 0.97, 95% CI: 0.94–1.01, p = 0.2111).ConclusionOur study found that the relationship between green space and the risk of new-onset stroke follows a L-shaped curve. A lower amount of green space is associated with an increased risk of new-onset stroke. These findings require confirmation in future studies.
The reliability and quality of short videos as health information of guidance for lymphedema: a cross-sectional study
Authors: Zhou Xuchuan , Ma Gejia , Su Xuefeng , Li Xinyi , Wang Wenfei , Xia Linxi , Yang Chen , Liu Bin 
Published: January 2025

BackgroundThe prevalence of lymphedema is rising, necessitating accurate diagnostic and treatment information for affected patients. Short video-sharing platforms facilitate access to such information but require validation regarding the reliability and quality of the content presented. This study aimed to assess the reliability and quality of lymphedema-related information on Chinese short video-sharing platforms.MethodsWe collected 111 video samples addressing the diagnosis and treatment of lymphedema from four platforms: TikTok, Bilibili, WeChat, and Microblog. Two independent surgeons evaluated each video for content comprehensiveness, quality (using the Global Quality Score), and reliability (using the modified DISCERN tool). The videos from different sources were subsequently compared and analyzed.ResultsOut of 111 videos analyzed, 66 (59.46%) were uploaded by medical professionals, including breast surgeons, vascular surgeons, plastic surgeons, physical therapists, and gynecologists, while 45 (40.54%) were shared by non-medical professionals such as science bloggers, medical institutions, and lymphedema patients. Patient-uploaded videos received the highest engagement, with median likes of 2,257 (IQR: 246.25–10998.25) and favorites of 399 (IQR: 94.5–1794.75). 13 videos (11.71%) contained inaccuracies. Medical professionals’ videos generally showed higher content comprehensiveness, particularly those by plastic surgeons, compared to non-medical professionals. The GQS and modified DISCERN tool were used to assess video quality and reliability respectively, with medical professionals scoring higher on both metrics (z = 3.127, p = 0.002; z = 2.010, p = 0.044). The quality and reliability of recommendations provided by plastic surgeons surpassed that of other medical professionals (χ2 = 16.196, p = 0.003; χ2 = 9.700, p = 0.046). No significant differences in video quality and reliability were found among the three categories of non-medical professionals (χ2 = 3.491, p = 0.175; χ2 = 2.098, p = 0.350).ConclusionOur study shows that lymphedema-related videos on short video platforms vary widely in quality. Videos by medical professionals are generally more accurate and of higher quality than those by non-professionals. However, patient-uploaded videos often get more engagement due to their relatability. To ensure public access to reliable information, establishing basic standards for this content is essential.
Effort-reward imbalance and health outcomes in emergency nurses: the mediating role of work–family conflict and intrinsic effort
Authors: Tong Le , Zhu Ling , Zhang Hao , Zhong Luying , Diao Dongmei , Chen Xiaoli , Zhang Jianna 
Published: January 2025

BackgroundEmergency nurses experience high stress, but the mechanisms linking effort-reward imbalance to health outcomes are unclear. Work–family conflict might mediate this relationship, and intrinsic effort could moderate it. This study aimed to explore these interactions and their impact on nurse health.MethodsA prospective cross-sectional survey was conducted from 30 tertiary hospitals across 19 provinces in mainland China. Participants completed validated scales to measure effort-reward imbalance, work–family conflict, somatic symptoms, and mental health symptoms. Statistical analyses, including mediation and moderation, were performed using the PROCESS macro to evaluate the relationships between the variables.ResultsIn this study, 1,540 emergency nurses were finally included. The analysis revealed that 80.5% of participants reported experiencing effort-reward imbalance, which was significantly associated with increased somatic symptoms and mental symptoms. Work–family conflict was identified as a significant mediator in the relationship between effort-reward imbalance and health outcomes, with a direct effect of effort-reward imbalance on overall symptoms (β = 0.554, p &lt; 0.001). Additionally, intrinsic effort moderated the impact of effort-reward imbalance on work–family conflict; moderate levels of emotional investment provided protection against burnout, while excessive intrinsic effort heightened the risk of mental health deterioration (β = −0.104, p &lt; 0.001).ConclusionThese findings underscored the urgent need for targeted interventions that address both effort-reward imbalance and work–family conflict in healthcare settings. Enhancing workplace conditions and supporting emotional engagement among emergency nurses are essential strategies for improving their well-being and reducing burnout risk. Future research should focus on longitudinal studies to assess the effectiveness of these interventions over time.
Physical activity and content in a variety of physically active learning: an observational case study of real-world practices
Authors: Johansen Jan-Michael , Mandelid Mathias Brekke , Reinboth Michael , Resaland Geir Kåre , Bratland-Sanda Solfrid 
Published: January 2025

BackgroundResearch on physically active learning (PAL) has mainly been investigated experimentally, where interventions have been introduced to study effects on, for example, physical activity (PA) levels. This might undermine real-world contexts and realistic PA levels when teachers have sustained PAL in their regular teaching practice for several years. The purpose of this study was to observe and describe the organization and content of a variety of teaching where PAL was enacted by experienced teachers and to describe the corresponding PA levels and PA intensity in real-world practices.MethodsFifty-eight pupils and four teachers from one primary school and one secondary school were enrolled across the first, sixth, and eighth grades. The pupils’ physical activity (PA) levels were assessed during 37 enacted physically active learning (PAL) segments within longer teaching lessons using waist-worn triaxial accelerometers. Evenson cut-off points were employed to define PA intensities. All enacted PAL were passively observed by the same observer regarding primary bodily movement, duration, subject, location, collaborative or individual work, and task orientation.ResultsOn average, PAL consisted of 57.8% ± 15.7% sedentary time, 22.9% ± 7.5% light intensity PA, and 19.3% ± 10.8% moderate-to-vigorous intensity PA, displaying a large variety in different PAL segments. More intensive and higher volumes of PA were evident when PAL was facilitated outdoors, in gyms, or in large stairways, while lower PA volumes and intensity were assessed when PAL was enacted inside the classroom. The primary movement in PAL was mainly running and/or walking (78.3%), while the PAL activity was mainly organized as group work (83.7%) in non-competitive tasks (97.3%).ConclusionsThis study provides novel insights by being the first to investigate the organization and content of PAL enacted by experienced teachers in their teaching and the corresponding PA levels in their real-world practices. The results displayed a large diversity in PA levels and intensities and may serve as a starting point to further investigate the coherency of PA levels and PAL content in schools with sustained PAL teaching.
Improvement in the prediction power of an astrocyte genome-scale metabolic model using multi-omic data
Authors: Angarita-Rodríguez Andrea , Mendoza-Mejía Nicolás , González Janneth , Papin Jason , Aristizábal Andrés Felipe , Pinzón Andrés 
Published: January 2025

IntroductionThe availability of large-scale multi-omic data has revolution-ized the study of cellular machinery, enabling a systematic understanding of biological processes. However, the integration of these datasets into Genome-Scale Models of Metabolism (GEMs) re-mains underexplored. Existing methods often link transcriptome and proteome data independently to reaction boundaries, providing models with estimated maximum reaction rates based on individual datasets. This independent approach, however, introduces uncertainties and inaccuracies.MethodsTo address these challenges, we applied a principal component analysis (PCA)-based approach to integrate transcriptome and proteome data. This method facilitates the reconstruction of context-specific models grounded in multi-omics data, enhancing their biological relevance and predictive capacity.ResultsUsing this approach, we successfully reconstructed an astrocyte GEM with improved prediction capabilities compared to state-of-the-art models available in the literature.DiscussionThese advancements underscore the potential of multi-omic inte-gration to refine metabolic modeling and its critical role in studying neurodegeneration and developing effective therapies.
Spatial Accessibility Analysis of Snake Antivenom
Authors: Hao Wenjie , He Lanfen , Song Xingyue , Wang Juntao , Hu Yanlan , Chen Yu , Lv Chuanzhu , Yan Shijiao 
Published: January 2025

ObjectivesTo analyze the spatial accessibility of antivenom immunizing agents equipped hospitals in Hainan Province.MethodsThis paper analyzes the spatial accessibility of medical institutions equipped with different types of snake antivenom using network analysis and two-step mobile search method, and evaluates the service level and spatial accessibility of medical institutions equipped with different types of antivenom immunizing agents in Hainan Province from the perspectives of both supply and demand.ResultsThe number of people in Hainan Province who need to spend more than 1 h to reach an Agkistrodon Hyalys antivenom, Naja antivenom, Bungarus Multicnctus antivenom, Agkistrodon Acutus antivenom equipped hospital, and equipped with antivenom for all species of snakes in the country is approximately 856,000, 231,300, 3,071,000, 2,666,000 and 4,721,000 people, respectively. In the results of accessibility of hospital beds/health technicians equipped with antivenom in Hainan Province, Haikou and Sanya cities had the highest accessibility.ConclusionThe accessibility of hospitals equipped with antivenom in Hainan Province is unevenly distributed, with areas of high accessibility in the southern and northern regions and accessibility in the rest of the country to be improved.
Antidepressant- and Anxiolytic-like Effects in Mice of Alkaloids from Aerial Parts of Argemone platyceras Link & Otto
Authors: Gómez-Patiño Mayra Beatriz , Estrada-Reyes Rosa , Hernández-Mendoza Héctor Hugo , Suarez-Rojas Ángela , Arrieta-Baez Daniel 
Published: January 2025

Background/Objectives: Argemone platyceras Link & Otto, an endemic plant of Mexico, is widely distributed in the central area of the country, mainly in the states of Tlaxcala, Puebla, and the State of Mexico. Ethnobotanical studies in different communities of these states have demonstrated that it is primarily used to treat diabetes and mental illnesses, such as “los nervios” (nerves) and “el ansia” (anxiety); these terms are used in traditional medicine, but it is accepted that they refer to anxiety disorders. This study aimed to validate the traditional use of aerial parts of A. platyceras Link & Otto in treating these illnesses. Methods: a standardized acidic method to obtain alkaloids was used to obtain an extract (AlkExt), which was tested in adult male Swiss Webster mice in the tail suspension (TST) and forced swimming (FST) tests. Results: AlkExt was analyzed using mass spectrometry techniques (DI-ESI and UHPLC-MS) to detect 2,3′,4,5′-Tetramethoxystilbene (m/z 301.14, 3%), scoulerine (m/z 328.16, 19.8%), tetrahydro-columbamine (m/z 342.17, 28.8%), 8-(hydroxymethyl)-2,10-dimethoxy-6,8,13,13a-tetrahydro-5H-isoquinolino[2,1-b]isoquinoline-1,11-diol (m/z 358.17, 22.8%), and glaucine (m/z 356.19, 11.1%); these were assayed in a single oral administration of AlkExt, which caused robust anxiolytic- and antidepressant-like effects without affecting the spontaneous ambulatory activity of the mice. Conclusions: The easy and standardized AlkExt analyzed in pharmaceuticals assays in this study strongly suggest its therapeutic potential to treat the comorbidity of anxiety and depression disorders and support further investigations in people with these diseases.
Characterisation of Staphylococcus aureus Strains and Their Prophages That Carry Horse-Specific Leukocidin Genes lukP/Q
Authors: Monecke Stefan , Burgold-Voigt Sindy , Feßler Andrea T. , Krapf Martina , Loncaric Igor , Liebler-Tenorio Elisabeth M. , Braun Sascha D. , Diezel Celia , Müller Elke , Reinicke Martin , Reissig Annett , Cabal Rosel Adriana , Ruppitsch Werner , Hotzel Helmut , Hanke Dennis , Cuny Christiane , Witte Wolfgang , Schwarz Stefan , Ehricht Ralf 
Published: January 2025

Leukocidins of Staphylococcus (S.) aureus are bicomponent toxins that form polymeric pores in host leukocyte membranes, leading to cell death and/or triggering apoptosis. Some of these toxin genes are located on prophages and are associated with specific hosts. The genes lukP/Q have been described from equine S. aureus isolates. We examined the genomes, including the lukP/Q prophages, of S. aureus strains belonging to clonal complexes CC1, CC350, CC816, and CC8115. In addition to sequencing, phages were characterised by mitomycin C induction and transmission electron microscopy (TEM). All lukP/Q prophages integrated into the lip2=geh gene, and all included also the gene scn-eq encoding an equine staphylococcal complement inhibitor. The lukP/Q prophages clustered, based on gene content and allelic variants, into three groups. One was found in CC1 and CC97 sequences; one was present mainly in CC350 but also in other lineages (CC1, CC97, CC133, CC398); and a third one was exclusively observed in CC816 and CC8115. Prophages of the latter group additionally included a rare enterotoxin A allele (sea320E). Moreover, a prophage from a CC522 goat isolate was found to harbour lukP. Its lukF component could be regarded as chimaera comprising parts of lukQ and of lukF-P83. A putative kinase gene of 1095 basepairs was found to be associated with equine strains of S. aureus. It was also localised on prophages. However, these prophages were different from the ones that carried lukP/Q, and three different integration sites of kinase-carrying phages were identified. These observations confirmed the presence of prophage-located important virulence-associated genes in equine S. aureus and that certain prophages might determine the host specificity of the staphylococcal strains they reside in.
Impact of Infant Massage on Weight Gain: A Study in Banjar District, South Kalimantan
Authors: Adha Aprilea Noor , Sofia Norlaila , Rohmah Prihatanti Nur , Rusmilawaty Rusmilawaty 
Published: January 2025

Background: Underweight in children under five is still a significant health problem in many parts of the world, especially in developing countries. One such intervention that has gained cultural significance is infant massage, a traditional practice that promotes relaxation, improves digestion, and enhances weight gain. This study seeks to evaluate the impact of infant massage on weight gain in infants under 24 months within Banjar Regency, a region where undernutrition presents a significant public health issue. While infant massage is traditionally practiced and believed to benefit infant health, its effectiveness remains debatable.
Methods: A quasi-experimental design with a control group involving 51 mother-infant pairs was employed. Participants were divided into an intervention group (n=17) that received infant massage training and performed it over three months and a control group (n=34) that received no such intervention. This research uses an observation form instrument. Infant weight gain was the primary outcome measure, with data analyzed using independent sample t-tests to assess differences between the two groups.
Results: No statistically significant difference was found in weight gain between the intervention and control groups (t = -0.415, p = 0.680). However, descriptive data revealed that 88.2% of infants in the intervention group experienced weight gain compared to 85.3% in the control group, indicating a potential trend toward positive effects of infant massage.
Conclusion: Despite the lack of statistical significance, the observed trend suggests potential benefits of infant massage. Further research with larger sample sizes and longer durations is recommended.