bluuebunny/crossref_metadata_2025_split huggingface dataset:
Microplastic pollution in African countries’ water systems: a review on findings, applied methods, characteristics, impacts, and managements
Authors: Aragaw Tadele Assefa 
Published: May 2021

Abstract
Owed to their simplicity, flexibility, lightweight, and low cost, plastics have become highly demanded in Africa as well as worldwide. However, the management of plastic wastes, particularly in African countries, is inadequate and most of the plastic debris is gatewayed into the water bodies. Nowadays, environmentalists, organizations, and governments are aware of microplastic pollution in the marine and terrestrial environment. Thus, addressing a compressive literature review in one referenced paper, as they draw up the articles, is essential to propose new research directions, to synthesize the existing theories among the existing studies. The abundance of microplastics is variable depending on the sampling and identification techniques. In this review, the available publications on microplastic pollution in African countries’ water systems were retrieved. Investigations found that microplastic pollution levels in the studied water bodies were reported in high concentrations. It was observed that different sampling and analytical methods were applied for the detection of microplastics, and suggestions were raised at it may affect the reliability of the results. Most of the detected and quantified microplastics were confirmed as they are from secondary sources. Most of the microplastic pollution research was conducted dominantly in South Africa, and secondly Nigeria, although other countries should also start conducting in their water systems. Surface water and sediment samples were dominantly carried out, but are limited with biota samples; hence, the risk assessment of microplastics is not yet determined. Some of the African countries have regulations on the prevention of macroplastic wastes, but the implementations are unsuccessful and most have not yet been established resulting in a threat of microplastics pollution. Thus, the research priorities on microplastic detection should be identified, and the African countries’ governments should be more proactive in eradicating macroplastic, which ends up as microplastics, pollutions in the water environments.

Graphic abstract

Article Highlights

Researches on microplastic pollution in African countries water system is limited .

A high microplastics abundance is found in African countries water system.

Sampling methods and used analytical techniques for microplastic detection were included.

Harmonized standard methods for microplastic pollution research should be established.

Combined analytical tools at once should be adopted to detect reliable microplastics.
Collegiate Principal Structures in Implementing a School Behaviour Support Program: The Role of the Local Management Group Model
Authors: Foggett Judith , Conway Robert , Dally Kerry 
Published: May 2021

AbstractStudent problem behaviour in schools is an issue for teachers, schools, and education jurisdictions. Problem behaviour also has an effect on families, the individual student, and the community. It is one of the principal issues of discussion for teachers, preservice teachers, principals, and policymakers. The purpose of this study was to examine a model that supports schools in managing available resources to promote positive behaviour and address problem behaviour. Interviews were conducted with 12 primary and high school principals to investigate their experiences of working together in 6 local management groups (LMGs) for the management of student behaviour. The findings revealed that the LMG model supported effective collaborative leadership practices between the high school and primary school principals and encouraged collegial networks among primary and high school teachers through joint professional learning opportunities. The benefits of the LMG model highlighted some positive outcomes for principals, teachers, and students with problem behaviour within their local schools.
Governance Quality, Fairness Perceptions, and Satisfaction with Democracy in Latin America
Authors: Saxton Gregory W. 
Published: May 2021

ABSTRACTHow do individuals’ fairness judgments aﬀect their political evaluations? This article argues that when citizens perceive high levels of distributive unfairness in society, they will be less satisfied with the way democracy functions. Yet good governance—that is, impartiality in the exercise of political authority—should mitigate the negative inﬂuence of perceived distributive unfairness on satisfaction. Using a cross-national analysis of 18 Latin American countries from 2011 to 2015, this study demonstrates that individuals are significantly less satisfied with democracy when they perceive their country’s income distribution as unfair. Yet good governance significantly oﬀsets this negative relationship, even in a region with the highest level of inequality in the world. These findings imply that policymakers can bolster democratic satisfaction, even in places where citizens perceive the income distribution as fundamentally unfair, by committing to good governance and fair democratic procedures.
Global extinction risk for seahorses, pipefishes and their near relatives (Syngnathiformes)
Authors: Pollom Riley A. , Ralph Gina M. , Pollock Caroline M. , Vincent Amanda C.J. 
Published: May 2021

Abstract
Few marine taxa have been comprehensively assessed for their conservation status, despite heavy pressures from fishing, habitat degradation and climate change. Here we report on the first global assessment of extinction risk for 300 species of syngnathiform fishes known as of 2017, using the IUCN Red List criteria. This order of bony teleosts is dominated by seahorses, pipefishes and seadragons (family Syngnathidae). It also includes trumpetfishes (Aulostomidae), shrimpfishes (Centriscidae), cornetfishes (Fistulariidae) and ghost pipefishes (Solenostomidae). At least 6% are threatened, but data suggest a mid-point estimate of 7.9% and an upper bound of 38%. Most of the threatened species are seahorses (Hippocampus spp.: 14/42 species, with an additional 17 that are Data Deficient) or freshwater pipefishes of the genus Microphis (2/18 species, with seven additional that are Data Deficient). Two species are Near Threatened. Nearly one-third of syngnathiformes (97 species) are Data Deficient and could potentially be threatened, requiring further field research and evaluation. Most species (61%) were, however, evaluated as Least Concern. Primary threats to syngnathids are (1) overexploitation, primarily by non-selective fisheries, for which most assessments were determined by criterion A (Hippocampus) and/or (2) habitat loss and degradation, for which assessments were determined by criterion B (Microphis and some Hippocampus). Threatened species occurred in most regions but more are found in East and South-east Asia and in South African estuaries. Vital conservation action for syngnathids, including constraining fisheries, particularly non-selective extraction, and habitat protection and rehabilitation, will benefit many other aquatic species.
Circulating microRNAs 34a, 122, and 192 are linked to obesity-associated inflammation and metabolic disease in pediatric patients
Authors: Lischka Julia , Schanzer Andrea , Hojreh Azadeh , Ba-Ssalamah Ahmed , de Gier Charlotte , Valent Isabella , Item Chike Bellarmine , Greber-Platzer Susanne , Zeyda Maximilian 
Published: May 2021

Abstract
Background
Obesity-associated chronic low-grade inflammation leads to dysregulation of central lipid and glucose metabolism pathways leading to metabolic disorders. MicroRNAs (miRNAs) are known to control regulators of metabolic homeostasis. We aimed to assess the relationship of circulating miRNAs with inflammatory modulators and metabolic disorders in pediatric obesity.

Methods
From a pediatric cohort with severe obesity (n = 109), clinically thoroughly characterized including diverse routine blood parameters, oral glucose tolerance test, and liver MRI, a panel of 16 circulating miRNAs was quantified using qRT-PCR. Additionally, markers of inflammation TNFα, IL1 receptor antagonist, procalcitonin, CRP, and IL-6 were measured.

Results
Markers of obesity-associated inflammation, TNFα, IL-1Ra, and procalcitonin, all significantly correlated with concentrations of miRNAs 122 and 192. Concentrations of these miRNAs negatively correlated with serum adiponectin and were among those strongly linked to parameters of dyslipidemia and liver function. Moreover, miRNA122 concentrations correlated with HOMA-IR. Several miRNA levels including miRNAs 34a, 93, 122, and 192 were statistically significantly differing between individuals with prediabetes, impaired glucose tolerance, metabolic syndrome, or nonalcoholic fatty liver disease compared to the respective controls. Additionally, miRNA 192 was significantly elevated in metabolically unhealthy obesity.

Conclusions
A miRNA pattern associated with obesity-associated inflammation and comorbidities may be used to distinguish metabolically healthy from unhealthy pediatric patients with obesity. Moreover, these changes in epigenetic regulation could potentially be involved in the etiology of obesity-linked metabolic disease in children and adolescents.
Association between perinatal factors, genetic susceptibility to obesity and age at adiposity rebound in children of the EDEN mother–child cohort
Authors: Cissé Aminata Hallimat , Lioret Sandrine , de Lauzon-Guillain Blandine , Forhan Anne , Ong Ken K. , Charles Marie Aline , Heude Barbara 
Published: May 2021

Abstract
Background
Early adiposity rebound (AR) has been associated with increased risk of overweight or obesity in adulthood. However, little is known about early predictors of age at AR. We aimed to study the role of perinatal factors and genetic susceptibility to obesity in the kinetics of AR.

Methods
Body mass index (BMI) curves were modelled by using mixed-effects cubic models, and age at AR was estimated for 1415 children of the EDEN mother–child cohort study. A combined obesity risk-allele score was calculated from genotypes for 27 variants identified by genome-wide association studies of adult BMI. Perinatal factors of interest were maternal age at delivery, parental education, parental BMI, gestational weight gain, maternal smoking during pregnancy, and newborn characteristics (sex, prematurity, and birth weight). We used a hierarchical level approach with multivariable linear regression model to investigate the association between these factors, obesity risk-allele score, and age at AR.

Results
A higher genetic susceptibility to obesity score was associated with an earlier age at AR. At the most distal level of the hierarchical model, maternal and paternal educational levels were positively associated with age at AR. Children born to parents with higher BMI were more likely to exhibit earlier age at AR. In addition, higher gestational weight gain was related to earlier age at AR. For children born small for gestational age, the average age at AR was 88 [±39] days lower than for children born appropriate for gestational age and 91 [±56] days lower than for children born large for gestational age.

Conclusion
The timing of AR seems to be an early childhood manifestation of the genetic susceptibility to adult obesity. We further identified low birth weight and gestational weight gain as novel predictors of early AR, highlighting the role of the intrauterine environment in the kinetics of adiposity.
Nuclear localisation of Aurora-A: its regulation and significance for Aurora-A functions in cancer
Authors: Naso Francesco Davide , Boi Dalila , Ascanelli Camilla , Pamfil Georgiana , Lindon Catherine , Paiardini Alessandro , Guarguaglini Giulia 
Published: May 2021

AbstractThe Aurora-A kinase regulates cell division, by controlling centrosome biology and spindle assembly. Cancer cells often display elevated levels of the kinase, due to amplification of the gene locus, increased transcription or post-translational modifications. Several inhibitors of Aurora-A activity have been developed as anti-cancer agents and are under evaluation in clinical trials. Although the well-known mitotic roles of Aurora-A point at chromosomal instability, a hallmark of cancer, as a major link between Aurora-A overexpression and disease, recent evidence highlights the existence of non-mitotic functions of potential relevance. Here we focus on a nuclear-localised fraction of Aurora-A with oncogenic roles. Interestingly, this pool would identify not only non-mitotic, but also kinase-independent functions of the kinase. We review existing data in the literature and databases, examining potential links between Aurora-A stabilisation and localisation, and discuss them in the perspective of a more effective targeting of Aurora-A in cancer therapy.
Metagenomic analysis reveals oropharyngeal microbiota alterations in patients with COVID-19
Authors: Ma Shengli , Zhang Fan , Zhou Fengxia , Li Hui , Ge Wenyu , Gan Rui , Nie Huan , Li Biao , Wang Yindong , Wu Meng , Li Duo , Wang Dongmei , Wang Zheng , You Yuhong , Huang Zhiwei 
Published: May 2021

AbstractCOVID-19 remains a serious emerging global health problem, and little is known about the role of oropharynx commensal microbes in infection susceptibility and severity. Here, we present the oropharyngeal microbiota characteristics identified by shotgun metagenomic sequencing analyses of oropharynx swab specimens from 31 COVID-19 patients, 29 influenza B patients, and 28 healthy controls. Our results revealed a distinct oropharyngeal microbiota composition in the COVID-19 patients, characterized by enrichment of opportunistic pathogens such as Veillonella and Megasphaera and depletion of Pseudopropionibacterium, Rothia, and Streptococcus. Based on the relative abundance of the oropharyngeal microbiome, we built a microbial classifier to distinguish COVID-19 patients from flu patients and healthy controls with an AUC of 0.889, in which Veillonella was identified as the most prominent biomarker for COVID-19 group. Several members of the genus Veillonella, especially Veillonella parvula which was highly enriched in the oropharynx of our COVID-19 patients, were also overrepresented in the BALF of COVID-19 patients, indicating that the oral cavity acts as a natural reservoir for pathogens to induce co-infections in the lungs of COVID-19 patients. We also found the increased ratios of Klebsiella sp., Acinetobacter sp., and Serratia sp. were correlated with both disease severity and elevated systemic inflammation markers (neutrophil–lymphocyte ratio, NLR), suggesting that these oropharynx microbiota alterations may impact COVID-19 severity by influencing the inflammatory response. Moreover, the oropharyngeal microbiome of COVID-19 patients exhibited a significant enrichment in amino acid metabolism and xenobiotic biodegradation and metabolism. In addition, all 26 drug classes of antimicrobial resistance genes were detected in the COVID-19 group, and were significantly enriched in critical cases. In conclusion, we found that oropharyngeal microbiota alterations and functional differences were associated with COVID-19 severity.
Hyperbaric oxygen promotes not only glioblastoma proliferation but also chemosensitization by inhibiting HIF1α/HIF2α-Sox2
Authors: Wang Pan , Gong Sheng , Pan Jinyu , Wang Junwei , Zou Dewei , Xiong Shuanglong , Zhao Lu , Yan Qian , Deng Yangming , Wu Nan , Liao Bin 
Published: May 2021

AbstractThere exists a consensus that combining hyperbaric oxygen (HBO) and chemotherapy promotes chemotherapy sensitivity in GBM cells. However, few studies have explored the mechanism involved. HIF1α and HIF2α are the two main molecules that contribute to GBM malignant progression by inhibiting apoptosis or maintaining stemness under hypoxic conditions. Moreover, Sox2, a marker of stemness, also contributes to GBM malignant progression through stemness maintenance or cell cycle arrest. Briefly, HIF1α, HIF2α and Sox2 are highly expressed under hypoxia and contribute to GBM growth and chemoresistance. However, after exposure to HBO for GBM, whether the expression of the above factors is decreased, resulting in chemosensitization, remains unknown. Therefore, we performed a series of studies and determined that the expression of HIF1α, HIF2α and Sox2 was decreased after HBO and that HBO promoted GBM cell proliferation through cell cycle progression, albeit with a decrease in stemness, thus contributing to chemosensitization via the inhibition of HIF1α/HIF2α-Sox2.
Minor changes in fibre intake in the UK population between 2008/2009 and 2016/2017
Authors: Gressier Mathilde , Frost Gary 
Published: May 2021

AbstractThe benefits of increasing populations’ and individuals’ fibre intake on non-communicable disease risk have been known and promoted for decades in the UK and in the world. Public health campaigns, including dietary recommendations, called populations to increase their consumption of whole grains, fruits and vegetables, while manufacturers increased the fibre content of their products. In particular, the SACN report in 2015 highlighted the importance of fibres for the UK population. We analysed trends in fibre consumption for the whole population, by age group and gender using the UK National Diet and Nutrition Survey from 2008/09 to 2016/17. We investigated changes in total fibre intake and calculated the contribution to fibre intake and time trends from each food group. We compared the fibre content of food groups between 2008/09 and 2016/17. We found that fibre intake remained fairly stable. While the fibre content of some cereal-based products increased, it decreased for potato-based products. All age groups derived increasing fibre from pasta and other cereal-based products, and decreasing fibre from potato products. Adults, but not children or adolescents derived more fibre from vegetables. This resulted in an increase in fibre intake in adults, but not in children or adolescents.
The landscape and driver potential of site-specific hotspots across cancer genomes
Authors: Juul Randi Istrup , Nielsen Morten Muhlig , Juul Malene , Feuerbach Lars , Pedersen Jakob Skou 
Published: May 2021

AbstractLarge sets of whole cancer genomes make it possible to study mutation hotspots genome-wide. Here we detect, categorize, and characterize site-specific hotspots using 2279 whole cancer genomes from the Pan-Cancer Analysis of Whole Genomes project and provide a resource of annotated hotspots genome-wide. We investigate the excess of hotspots in both protein-coding and gene regulatory regions and develop measures of positive selection and functional impact for individual hotspots. Using cancer allele fractions, expression aberrations, mutational signatures, and a variety of genomic features, such as potential gain or loss of transcription factor binding sites, we annotate and prioritize all highly mutated hotspots. Genome-wide we find more high-frequency SNV and indel hotspots than expected given mutational background models. Protein-coding regions are generally enriched for SNV hotspots compared to other regions. Gene regulatory hotspots show enrichment of potential same-patient second-hit missense mutations, consistent with enrichment of hotspot driver mutations compared to singletons. For protein-coding regions, splice-sites, promoters, and enhancers, we see an excess of hotspots associated with cancer genes. Interestingly, missense hotspot mutations in tumor suppressors are associated with elevated expression, suggesting localized amino-acid changes with functional impact. For individual non-coding hotspots, only a small number show clear signs of positive selection, including known sites in theTERTpromoter and the 5’ UTR ofTP53. Most of the new candidates have few mutations and limited driver evidence. However, a hotspot in an enhancer of the oncogenePOU2AF1, which may create a transcription factor binding site, presents multiple lines of driver-consistent evidence.
Nano-imaging of strain-tuned stripe textures in a Mott crystal
Authors: McLeod A. S. , Wieteska A. , Chiriaco G. , Foutty B. , Wang Y. , Yuan Y. , Xue F. , Gopalan V. , Chen L. Q. , Mao Z. Q. , Millis A. J. , Pasupathy A. N. , Basov D. N. 
Published: May 2021

AbstractThe 4d transition metal perovskites Can+1RunO3n+1 have attracted interest for their strongly interacting electronic phases showing pronounced sensitivity to controllable stimuli like strain, temperature, and even electrical current. Through multi-messenger low-temperature nano-imaging, we reveal a spontaneous striped texture of coexisting insulating and metallic domains in single crystals of the bilayer ruthenate Ca3(TixRu1-x)2O7 across its first-order Mott transition at $$T \approx 95$$

T
≈
95

K. We image on-demand anisotropic nucleation and growth of these domains under in situ applied uniaxial strain rationalized through control of a spontaneous Jahn-Teller distortion. Our scanning nano-susceptibility imaging resolves the detailed susceptibility of coexisting phases to strain and temperature at the transition threshold. Comparing these nano-imaging results to bulk-sensitive elastoresistance measurements, we uncover an emergent “domain susceptibility” sensitive to both the volumetric phase fractions and elasticity of the self-organized domain lattice. Our combined susceptibility probes afford nano-scale insights into strain-mediated control over the insulator-metal transition in 4d transition metal oxides.
Fentanyl conjugate vaccine by injected or mucosal delivery with dmLT or LTA1 adjuvants implicates IgA in protection from drug challenge
Authors: Stone Addison E. , Scheuermann Sarah E. , Haile Colin N. , Cuny Gregory D. , Velasquez Marcela Lopez , Linhuber Joshua P. , Duddupudi Anantha L. , Vigliaturo Jennifer R. , Pravetoni Marco , Kosten Therese A. , Kosten Thomas R. , Norton Elizabeth B. 
Published: May 2021

AbstractFentanyl is a major contributor to the devastating increase in overdose deaths from substance use disorders (SUD). A vaccine targeting fentanyl could be a powerful immunotherapeutic. Here, we evaluated adjuvant and delivery strategies for conjugate antigen vaccination with fentanyl-based haptens. We tested adjuvants derived from the heat-labile toxin of E. coli including dmLT and LTA1 by intramuscular, sublingual or intranasal delivery. Our results show anti-fentanyl serum antibodies and antibody secreting cells in the bone-marrow after vaccination with highest levels observed with an adjuvant (alum, dmLT, or LTA1). Vaccine adjuvanted with LTA1 or dmLT elicited the highest levels of anti-fentanyl antibodies, whereas alum achieved highest levels against the carrier protein. Vaccination with sublingual dmLT or intranasal LTA1 provided the most robust blockade of fentanyl-induced analgesia and CNS penetration correlating strongly to anti-FEN IgA. In conclusion, this study demonstrates dmLT or LTA1 adjuvant as well as mucosal delivery may be attractive strategies for improving the efficacy of vaccines against SUD.