bluuebunny/crossref_metadata_2025_split huggingface dataset:
Generation of iPSC lines with high cytogenetic stability from peripheral blood mononuclear cells (PBMCs)
Authors: Panther Lindsay , Ornelas Loren , Jones Michelle R , Gross Andrew R. , Gomez Emilda , Liu Chunyan , Berman Benjamin , Svendsen Clive N. , Sareen Dhruv 
Published: September 2021

SUMMARYThe utility of human induced pluripotent stem cells (hiPSCs) is contingent upon genomic integrity and stability. Recurrent genomic aberrations have been observed in human iPSC lines upon long-term culture, ∼10-25% demonstrate karyotype abnormalities. We describe a new and reliable non-integrating episomal plasmid reprogramming method for fresh (unexpanded) peripheral blood mononuclear cells (PBMC) into iPSCs (PBMC-iPSCs). PBMC-iPSCs produced using this method have a superior chromosome-level karyotype stability rate (∼5% abnormality rate for all chromosomes; 2.8% for autosomes). After extended culture PBMC-iPSCs maintain a low rate of abnormalities (2% for autosomes). Deep coverage whole genome sequencing in a subset of PBMC-iPSC lines showed no shared single nucleotide polymorphisms (SNPs) or structural variants are introduced during reprogramming and maintenance of PBMC-iPSCs. iPSCs reprogrammed from unexpanded PBMCs have consistently high cytogenetic stability and minimal genomic aberrations, suggesting this method is highly suited for iPSCs in research and therapeutic clinical applications.
Inhibition of chloroplast translation as a new target for herbicides
Authors: Sukhoverkov Kirill V. , Breese Karen J. , Debowski Aleksandra W. , Murcha Monika W. , Stubbs Keith A. , Mylne Joshua S. 
Published: September 2021

AbstractThe rise in herbicide resistance over recent decades threatens global agriculture and food security and so discovery of new modes of action is increasingly important. Here we reveal linezolid, an oxazolidinone antibiotic that inhibits microbial translation, is also herbicidal. To validate the herbicidal mode of action of linezolid we confirmed its micromolar inhibition is specific to chloroplast translation and did not affect photosynthesis directly. To assess the herbicide potential of linezolid, testing against a range of weed and crop species found it effective pre- and post-emergence. Using structure-activity analysis we identified the critical elements for herbicidal activity, but importantly also show, using antimicrobial susceptibility assays, that separation of antibacterial and herbicidal activities was possible. Overall these results validate chloroplast translation as a viable herbicidal target.
The modifying enzyme Tsr3 establishes the hierarchy of Rio kinase activity in 40S ribosome assembly
Authors: Huang Haina , Parker Melissa , Karbstein Katrin 
Published: September 2021

AbstractRibosome assembly is an intricate process, which in eukaryotes is promoted by a large machinery comprised of over 200 assembly factors (AF) that enable the modification, folding, and processing of the ribosomal RNA (rRNA) and the binding of the 79 ribosomal proteins. While some early assembly steps occur via parallel pathways, the process overall is highly hierarchical, which allows for the integration of maturation steps with quality control processes that ensure only fully and correctly assembled subunits are released into the translating pool. How exactly this hierarchy is established, in particular given that there are many instances of RNA substrate “handover” from one highly related AF to another remains to be determined. Here we have investigated the role of Tsr3, which installs a universally conserved modification in the P-site of the small ribosomal subunit late in assembly. Our data demonstrate that Tsr3 separates the activities of the Rio kinases, Rio2 and Rio1, with whom it shares a binding site. By binding after Rio2 dissociation, Tsr3 prevents rebinding of Rio2, promoting forward assembly. After rRNA modification is complete, Tsr3 dissociates, thereby allowing for recruitment of Rio1. Inactive Tsr3 blocks Rio1, which can be rescued using mutants that bypass the requirement for Rio1 activity. Finally, yeast strains lacking Tsr3 randomize the binding of the two kinases, leading to the release of immature ribosomes into the translating pool. These data demonstrate a role for Tsr3 and its modification activity in establishing a hierarchy for the function of the Rio kinases.
The regional economy of Central Macedonia: an application of the social accounting matrix
Authors: Maniati Anthia , Loizou Efstratios , Psaltopoulos Dimitrios , Mattas Konstadinos 
Published: September 2021

PurposeThe economic and social problems, including high unemployment, facing the Greek economy in recent years are substantial. The role of the agri-food sector and agriculture in dealing with unemployment is a concern. To support the agricultural economy, a Common Agricultural Policy (CAP) 2007–2014 was adopted and implemented in the EU Member States. However, boosting employment, in the industry itself and indirectly in the economy, has almost never been a key goal of any policy. The purpose of this paper is to examine the role of the CAP 2014–2020 in maintaining and enhancing employment and income distribution in the region of Central Macedonia.Design/methodology/approachThe authors used the regional social accounting matrix (SAM), which examines the depiction of the interconnections between the sectors of economic activity and local economy (households, businesses, public) but also interconnections and transactions with the rest of the world. The SAM presents a more complete picture of the economic figures of the region, evaluating the interconnections of cross-sectoral relations and the implemented policies, both in the production sectors and in the regional society.FindingsFor the Central Macedonia region, the agricultural sector is a key player, holding a regulatory role for regional economic viability, and shows marked connections with the other industry branches of the region.Originality/valueThe new CAP 2014–2020 through Pillar 2–Rural Development may reinforce the new role of the industry in terms of the environment, integrated rural development and social structure of rural areas, ensuring coherence.
Risk-taking behavior, competition, diversification and performance of frontier and emerging economy banks
Authors: Boamah Nicholas Addai , Boakye-Dankwa Augustine , Opoku Emmanuel 
Published: September 2021

PurposeThe study examines the dynamic association between competition, risk-taking, performance and income diversification of frontier and emerging economy (FEE) banks. It additionally, explores the effect of banking sector depth and economic performance on the level of competition, performance and risk-taking behavior of banks in these economies.Design/methodology/approachThe paper adopts a panel vector auto-regressive technique and collects data across ninety (90) FEEs.FindingsThe paper finds that competition increases with improvement in the depth of the banking sector, a surge in risk-taking behavior and the adoption of focused strategy by banks. Similarly, income diversification activities are driven by competition, banking sector depth, the state of the economy and bank performance. Additionally, risk-taking behavior, banking sector depth and the state of the economy are relevant in describing bank performance. Also, risk-taking behavior is influenced by bank performance, banking sector depth and economic growth.Originality/valueThe evidence indicates that although competition improves banking sector health, excessive competition and non-competitive banking environment constrain banks’ performance and stability.
What attracts me or prevents me from mobile shopping? An adapted UTAUT2 model empirical research on behavioral intentions of aspirant young consumers in Pakistan
Authors: Hanif Muhammad Shehzad , Wang Min , Mumtaz Muhammad Usman , Ahmed Zeeshan , Zaki Waqas 
Published: September 2021

PurposeAcceptance and use of mobile shopping as the preferred shopping medium is becoming the new normal today. The proliferation of mobile shopping practices follows unsteady growth patterns in different countries. Despite challenges of price, quality and privacy matters, young consumers still lead the race to engage in mobile shopping activity in developing countries. This research investigates the determinants that either support or hinder the mobile shopping intentions of aspirant young consumers in Pakistan.Design/methodology/approachThe present research applies a consumer-centric approach to the technology adoption framework to unearth the behavioral patterns of these young consumers in Pakistan. Employing the structural equation modeling technique, this research examines the significant effect of structural assurance, perceived risk, trust and various unified theory of acceptance and use of technology (UTAUT) model constructs on behavioral intention to engage in mobile shopping.FindingsThis research examines the significant effect of structural assurance, perceived risk, trust and various UTAUT model constructs on behavioral intention to engage in mobile shopping. Results also demonstrate a significant moderating effect of structural assurance and prior shopping experience on the relationship between perceived risk, trust and mobile shopping intentions. Further, the mobile shopping patterns for the male and female segment indicate a significant difference for perceived risk, trust structural assurance and social influence. The research contributes to the growing body of knowledge which advocates the application of consumer-centric customized model approach to explore various factors that either facilitate or impede the adoption of mobile shopping in a developing economy.Research limitations/implicationsThe study validates the need to enforce structural assurance mechanism for facilitating mobile shopping in a developing country. It also offers practical implications for online businesses and marketers striving to attract and retain more mobile customers.Practical implicationsThe study offers practical implications for online businesses and marketers striving to attract and retain more mobile customers.Originality/valueThis study offers fresh insights about driving elements and impediments of mobile shopping behavioral intentions. Structural assurance and prior shopping experience appear to influence the mobile shopping behavior through direct and indirect effect.
Experimental validation of a topological derivative-based crack growth control method using digital image correlation
Authors: Barros Guilherme , Filho João , Nunes Luiz , Xavier Marcel 
Published: September 2021

PurposeThe purpose of this paper is to experimentally validate the crack growth control based on the topological derivative of the famous Rice's integral.Design/methodology/approachSingle edge notch tensile specimens with two configurations were tested. Displacement fields near notch were experimentally obtained using the digital image correlation method. These displacements were used to verify the minimization of the associated shape functional, which is defined in terms of the Rice's integral, when a set of controls (holes) positioned according to the topological derivative information, is inserted. Based on the Griffth's energy criterion, this minimization represents an improvement in the fracture toughness of cracked bodies.FindingsThe experimental tests confirmed that a decrease around 27% in the value of the associated shape functional can be obtained following this approach. Therefore, the results allow us to conclude that the predictive methodology for crack growth control based on the topological derivative is feasible.Originality/valueThis is the first work concerning experimental validation of crack growth control based on the topological derivative method.
Innovation structure combining inter-story isolation with passive cooling effect for AP1000 nuclear power plants
Authors: Hou Gangling , Liu Yu , Wang Tao , Wang Binsheng , Song Tianshu , Sun Menghan , Li Yong 
Published: September 2021

PurposeAn inter-story isolation structure (IIS) for AP1000 nuclear power plants (NPPs) is provided to resolve the conflict of seismic safety and the optimal location of air intakes.Design/methodology/approachThe effect of passive cooling system (PCS) is better with lower altitude of air intakes than that in the original design of AP1000 NPPs. Seismic performances of IIS NPPs, including the seismic responses, damping frequency bandwidth and seismic reduction robustness, are improved by combining the position of air intakes lower and the optimal design method.FindingsTheoretical analysis and numerical simulation are illustrated that the seismic reduction failure of IIS NPPs is the lowest probability of occurrence when PCS has highest working efficiency.Originality/valueThe IIS NPPs can transfer the contradiction between PCS work efficiency and seismic safety of NPPs to the mutual promotion of them.
Motivation to learn, mobile learning and online learning climate: moderating role of learner interaction
Authors: Swain Deepika , Jena Lalatendu Kesari , Dash Sanket Sunand , Yadav Rama Shankar 
Published: September 2021

Purpose
The purpose of this paper is to empirically exhibit the moderating effect of learner interaction (LI) on motivation to learn (MTL), mobile learning (ML) and online learning climate (OLC), so as to bring in enhanced rigour to the virtual knowledge dissemination during the times of crisis.

Design/methodology/approach
A total of 784 valid responses were considered for the confirmatory factor analysis to test the proposed hypotheses.

Findings
The study found that MTL and ML contributed to improved OLC and high LI moderated the positive relationship between MTL, ML and OLC. LI also directly contributed to an improved OLC.

Practical implications
Measures need to be designed to crowbar motivation to ensure heightened interaction of learners, to gear up the ML reach soaring heights achieving a dynamic OLC. Acclimatization of the OLC will be the visionary solution to tackle learning disruption during today’s pandemic times and also many other challenges to come in near-far future.

Originality/value
The current study established the moderating role of LI in influencing OLC, and also motivating facilitator’s for designing upgraded content, and thereby fuelling the intention to learn.
Understanding tenants’ motivations for co-living arrangements: a case study of Klang Valley, Malaysia
Authors: Christy Vania , Tan Teck Hong 
Published: September 2021

Purpose
The purpose of this study is to fill a knowledge gap by analyzing the motivations of tenants to co-living spaces in Klang Valley, Malaysia as the motives of co-living spaces are related to how well that space supports their needs.

Design/methodology/approach
Tenants’ behaviors were examined using a convergent parallel mixed-method approach, which included a survey and an in-depth interview. A total of 175 respondents were interviewed using purposive sampling.

Findings
The results show that the preference for co-living attributes has changed during the pandemic. User ratings of preference for physical and leasing attributes of co-living spaces are significant in terms of co-living motivations. The findings also revealed that tenants prefer twin-sharing and master bedrooms when choosing a co-living space to stay in.

Research limitations/implications
Identifying the factors that influence such motivations is critical for housing developers and co-living service providers to pay close attention to improving tenants’ living experiences.

Originality/value
There is interest in the co-living spaces that are available for rent. However, very little research is based on an understanding of how the tenants in Klang Valley, Malaysia perceive this type of living arrangement. A better understanding and prediction of tenants’ needs and preferences may lead to a better understanding of the attributes that influence their motivations for using co-living spaces.
Women empowerment through entrepreneurship: case study of a social entrepreneurial intervention in rural India
Authors: Agrawal Anirudh , Gandhi Poonam , Khare Prajakta 
Published: September 2021

Purpose
The purpose of this study is to explore how social entrepreneurship empowers women and how it influences the existing gender based intersectionalities in the society. This paper is based on a single case study of a social entrepreneurial startup called “Pahal,” which used social entrepreneurial strategies to encourage women to engage in economic activities, which helped them in gaining economic empowerment.

Design/methodology/approach
The researcher alongside the social entrepreneur visited the field for eight months from August 2016 to March 2017 and interviewed multiple stakeholder-employees (as partners) of the social enterprise. This study was done by conducting interviews with all the stakeholders in all the three social entrepreneurial phases, i.e. pre-Pahal, during-Pahal and after-Pahal. The interviews and observations were recorded and analysed using social entrepreneurial lens and intersectionality.

Findings
“Pahal Initiative” – is a social entrepreneurial intervention that helped the women in the household to start a food delivery business with the support of the social entrepreneur. Consequently, women gained confidence and self-respect. The attitude of men in the household changed when the women in the household generated additional income. It impacted their position and status which led to an increase in their participation in decision-making in the household and economic independence. The women become more interactive and expressive in a predominantly patriarchal household. Within one year, the entrepreneur had to stop the Pahal initiative. Then, this study observed and recorded that this event curtailed the economic activities undertaken by the women in their village and their social status reverted to what it was before the initiative.

Practical implications
First, social entrepreneurial strategies lead to economic value creation and lead overall socio-economic gains. Second, social entrepreneurial strategies address the problems of patriarchy and gender discrimination. Third, economic activities undertaken by women changed the social perceptions of the family members towards women in the families.

Social implications
From this case study, one observes that social entrepreneurship has a strong potential to bring about social and economic change. This study helps policymakers and non-governmental organisations to solve poverty and gender discrimination related problems using social entrepreneurial strategies.

Originality/value
This study uses social entrepreneurial intervention to understand and bring about change in the socio-economic status of women in rural India. This study uses an intersectionality lens to make sense of the data, reality and reflects on how intersectional positions are altered when women are economically empowered either through training or through a proper organisation or both.
Task scheduling and resource allocation of seasonal requests of users in cloud using NMKA and CM-GA techniques
Authors: Prathiba S. , Sankar Sharmila 
Published: September 2021

Purpose
The purpose of this paper is to provide energy-efficient task scheduling and resource allocation (RA) in cloud data centers (CDC).

Design/methodology/approach
Task scheduling and RA is proposed in this paper for cloud environment, which schedules the user’s seasonal requests and allocates resources in an optimized manner. The proposed study does the following operations: data collection, feature extraction, feature reduction and RA. Initially, the online streaming data of seasonal requests of multiple users were gathered. After that, the features are extracted based on user requests along with the cloud server, and the extracted features are lessened using modified principal component analysis. For RA, the split data of the user request is identified and that data is pre-processed by computing closed frequent itemset along with entropy values. After that, the user requests are scheduled using the normalized K-means algorithm (NKMA) centered on the entropy values. Finally, the apt resources are allotted to that scheduled task using the Cauchy mutation-genetic algorithm (CM-GA). The investigational outcomes exhibit that the proposed study outruns other existing algorithms in respect to response time, execution time, clustering accuracy, precision and recall.

Findings
The proposed NKMA and CM-GA technique’s performance is analyzed by comparing them with the existing techniques. The NKMA performance is analyzed with KMA and Fuzzy C-means regarding Prc (Precision), Rca (Recall), F ms (f measure), Acr (Accuracy)and Ct (Clustering Time). The performance is compared to about 500 numbers of tasks. For all tasks, the NKMA provides the highest values for Prc, Rca, Fms and Acr, takes the lowest time (Ct) for clustering the data. Then, the CM-GA optimization for optimally allocating the resource in the cloud is contrasted with the GA and particle swarm optimization with respect to Rt (Response Time), Pt (Process Time), Awt (Average Waiting Time), Atat (Average Turnaround Time), Lcy (Latency) and Tp (Throughput). For all number of tasks, the proposed CM-GA gives the lowest values for Rt, Pt, Awt, Atat and Lcy and also provides the highest values for Tp. So, from the results, it is known that the proposed technique for seasonal requests RA works well and the method optimally allocates the resources in the cloud.

Originality/value
The proposed approach provides energy-efficient task scheduling and RA and it paves the way for the development of effective CDC.