bluuebunny/crossref_metadata_2025_split huggingface dataset:
Learning the full impact of migraine through patient voices: A qualitative study
Authors: Estave Paige M. , Beeghly Summerlyn , Anderson Reid , Margol Caitlyn , Shakir Mariam , George Geena , Berger Anissa , O’Connell Nathaniel , Burch Rebecca , Haas Niina , Powers Scott W. , Seng Elizabeth , Buse Dawn C. , Lipton Richard B. , Wells Rebecca Erwin 
Published: June 2021

AbstractObjectiveTo better characterize the ways that migraine affects multiple domains of life.BackgroundFurther understanding of migraine burden is needed.MethodsAdults with migraine randomized to mindfulness‐based stress reduction or headache education arms (n = 81) in two separate randomized clinical trials participated in semistructured in‐person qualitative interviews conducted after the interventions. Interviews queried participants on migraine impact on life and were audio‐recorded, transcribed, and summarized into a framework matrix. A master codebook was created until meaning saturation was reached and magnitude coding established code frequency. Themes and subthemes were identified using a constructivist grounded theory approach.ResultsDespite most participants being treated with acute and/or prophylactic medications, 90% (73/81) reported migraine had a negative impact on overall life, with 68% (55/81) endorsing specific domains of life impacted and 52% (42/81) describing impact on emotional health. Six main themes of migraine impact emerged: (1) global negative impact on overall life; (2) impact on emotional health; (3) impact on cognitive function; (4) impact on specific domains of life (work/career, family, social); (5) fear and avoidance (pain catastrophizing and anticipatory anxiety); and (6) internalized and externalized stigma. Participants reported how migraine (a) controls life, (b) makes life difficult, and (c) causes disability during attacks, with participants (d) experiencing a lack of control and/or (e) attempting to push through despite migraine. Emotional health was affected through (a) isolation, (b) anxiety, (c) frustration/anger, (d) guilt, (e) mood changes/irritability, and (f) depression/hopelessness. Cognitive function was affected through concentration and communication difficulties.ConclusionsMigraine has a global negative impact on overall life, cognitive and emotional health, work, family, and social life. Migraine contributes to isolation, frustration, guilt, fear, avoidance behavior, and stigma. A greater understanding of the deep burden of this chronic neurological disease is needed to effectively target and treat what is most important to those living with migraine.
Hemophagocytic histiocytosis in severe SARS‐CoV‐2 infection: A bone marrow study
Authors: Dandu Himanshu , Yadav Geeta , Malhotra Hardeep Singh , Pandey Saurabh , Sachu Ruovinuo , Dubey Kinjalk 
Published: June 2021

AbstractIntroductionThe clinical and laboratory features of severe COVID‐19 infection overlap with those of hemophagocytic lymphohistiocytosis (HLH), a hyperinflammatory disorder often associated with several viral infections. The clinical syndrome of HLH encompasses fever, organomegaly, cytopenias, hyperferritinemia, hypertriglyceridemia, raised transaminases, hypofibrinogenemia, absent natural killer (NK) cell activity, increased soluble CD25 and hemophagocytic lymphohistiocytosis in bone marrow, spleen, and lymph nodes.MethodsWe analyzed clinicopathological and laboratory features of thirteen patients with severe COVID‐19 infection suspected to have HLH and found to have hemophagocytic histiocytosis on bone marrow examination (BME).ResultsFive of thirteen (38.46%) patients fulfilled five of eight HLH 2004 criteria and/or had a H‐score ≥169. Three (23.08%) satisfied four of eight and remainder five (38.46%) satisfied three of eight HLH 2004 criteria. Fever, raised serum ferritin (13/13, 100%), transaminases (9/13, 69.23%), triglycerides (4/13, 30.76%), cytopenias (5/13, 38.46%), hypofibrinogenemia (2/13, 15.38%), and organomegaly (1/13, 7.69%) were observed in our patients. BME showed hemophagocytic histiocytosis without lymphocytosis in all. Contrary to HLH, lymphocytopenia (11/13, 84.61%), leukocytosis (7/13, 53.84%), neutrophilia (7/13, 53.84%), and hyperfibrinogenemia (7/13, 53.84%) were observed. Serum CRP, LDH, and plasma D‐dimer were elevated in all, while serum albumin was decreased in 12 of 13 (92.3%) patients. Five patients recovered with high‐dose pulsed corticosteroid therapy.ConclusionThe immune response associated with severe COVID‐19 infection is similar to HLH with few differences. HLH should be suspected in severe COVID‐19 infection although all patients may not fulfill required HLH diagnostic criteria. BME should be done in suspected cases so that appropriate therapy may be initiated early.
Effects of online stigma‐reduction programme for people experiencing mental health conditions: A systematic review and meta‐analysis
Authors: Goh Yong‐Shian , Ow Yong Qing Yun Jenna , Tam Wai‐San Wilson 
Published: June 2021

AbstractDespite the increased awareness of mental health‐related issues, people experiencing mental health conditions have continued to face stigmatization worldwide. The literature on help‐seeking behaviours has frequently highlighted the development of self‐stigma because of public stigma and emphasized the need to address public stigmatization faced by them. Given the increasing acceptance of digital services in recent years, this systematic review aimed to examine the effects of online and face‐to‐face anti‐stigma interventions in reducing public stigma towards people experiencing mental health conditions. A search was conducted on the Cochrane Library, CINAHL, PubMed, Embase, PsycInfo, and ProQuest from inception of the databases to October 2020. Studies were included in this review if they have explored: (i) public stigmatization towards people of all ages with different types of mental health conditions; (ii) online interventions; and (iii) face‐to‐face interventions. Nine studies were included in this review, of which only five were included in the meta‐analysis as the remaining four had incomplete data. The meta‐analysis included an aggregate of 1203 participants while the four excluded studies included 713 participants. Results revealed that online interventions performed favourably with small effect sizes in comparison to face‐to‐face, wait‐list control, and no‐intervention groups. Results from the studies excluded from the meta‐analysis also found a significant reduction of public stigmatization with online interventions. Such findings provide insightful evidence for the effectiveness of online interventions in reducing public stigmatization. Hence, mental health organizations and groups can consider adopting online interventions suitable for their target audience and type of mental health conditions.
Reactive hot pressing of TiC0.5 ceramic at low applied pressure with 1 wt% Ni additive
Authors: Kannan Rajaguru , Rangaraj Lingappa , Jayaram Vikram 
Published: June 2021

AbstractDensification in non‐stoichiometric TiC0.5 ceramic has been studied by reactive hot pressing (RHP) of Ti:0.5C composition at 4–40 MPa, 1200°C for 60 min. Incomplete reaction and 94% relative density (RD) at a pressure of 4 MPa changed to 99% RD and a negligible amount of residual Ti at 40 MPa. In contrast, the addition of 1 wt% Ni in the starting Ti‐0.5C powder mixture resulted in full density at a lower pressure of 4 MPa, leading to comparable hardness. It is argued that both, reaction as well as densification, were improved by the formation of transient Ti‐Ni liquid phase. The enhanced RD and residual metallic phase in the nickel‐containing non‐stoichiometric TiC0.5 showed high flexural strength (537 ± 79 MPa), which is comparable to values obtained from materials processed at high temperature and pressure.
Broadening emission band of Yb:LuScO3 transparent ceramics for ultrashort pulse laser
Authors: Wang Wei , Jiang Benxue , Feng Tao , Fan Jintai , Ma Wanqiu , Guo Weian , Li Jiang , Zhang Long 
Published: June 2021

AbstractSesquioxides were considered as promising gain medium for high power ultrafast laser. Broadening of emission spectrum plays a crucial role in shortening the pulse width of laser. In this paper, the ratio of lutetium oxide and scandium oxide was adjusted to control the emission wavelength and spectral width. Highly sinterable Yb:(LuxSc1–x)2O3 nanopowders were synthesized through the glycine‐nitrate combustion process. Transparent Yb:(LuxSc1–x)2O3 ceramics were fabricated via vacuum sintering at 1750℃ for 10 h, among which Yb:(Lu0.5Sc0.5)2O3 ceramics have the highest transmittance, reaching 78% at 1100 nm. With increasing Lu2O3 content, the emission wavelengths corresponding to the two transitions of Yb:(LuxSc1–x)2O3 were blueshifted 8 and 15 nm, respectively. Most importantly, from Yb:Sc2O3 to Yb:(Lu0.5Sc0.5)2O3, spectral broadening of the two emission wavelengths was observed by 9 and 16 nm, respectively. Thus, the Yb:(LuxSc1‐x)2O3 ceramics exhibiting high transmittance and broad emission band are promising for ultrashort pulse generation.
Community cohesion during the first peak of the COVID‐19 pandemic: A social antidote to health anxiety and stress
Authors: Svensson Sarah Jane , Elntib Stamatis 
Published: June 2021

AbstractSocial support gained through community ties has been pivotal in dealing with stressful events. A cross‐sectional community sample (N = 2,329) was gathered to assess community cohesion buffering against heath anxiety and perceived stress during the first peak of the pandemic in the UK, using structural equation modeling analyses. Community cohesion acted as a protective mechanism against both health anxiety and stress during the first national lockdown. A strong positive association was also found between health anxiety and stress. Stress and health anxiety scores peaked in the first weeks of the imposed quarantine; as the lockdown was extended, participants reported lower stress, health anxiety and community cohesion scores. The reduction of community cohesion scores was greater for those younger than 45 while the positive association between stress and health anxiety was stronger among males during the lockdown. While community cohesion effects against health anxiety were enhanced for females, community's buffering against stress were greater for males. Strengthening citizens' psychological sense of community through the publicization and support of local initiatives and mutual‐aid groups and utilizing methodically green (and blue) spaces to boost neighborhood attraction might be viable strategies within which stress and health anxiety can be suppressed. Conversely, allowing community, regional and national cracks to deepen can exacerbate the impact of stressful events experienced during the COVID‐19 pandemic.
Flecainide‐induced AV dyssynchrony and atrial latency progression in a patient with a dual‐chamber pacemaker
Authors: Alston Michael , Chang David , Mitra Raman 
Published: June 2021

AbstractIntroductionFlecainide is a class 1C antiarrhythmic primarily usedfor the management of supraventricular arrhythmias. Adverse effects in patientswith pacemakers are poorly described in the current literature. We report acase of worsening intracardiac conduction in a patient with baseline atriallatency.CaseA patient with paroxysmalatrial fibrillation and recently implanted dual chamber pacemaker withresultant atrial latency underwent flecainide treatment resulting in worseningatrial latency, progressive dyspnea, and pacemaker syndrome physiology. The patientexperienced symptomatic improvement with pacemaker reprogramming to AAI andreduced flecainide dosages.ConclusionPrudence should be exercised when initiating flecainide therapy, particularly in pacemaker‐dependentpatients, who may be at risk for increasedthresholds, inconsistent capture, and latency. In the setting of apparentcapture latency, changing to AAI mode may both confirm the diagnosis andprovide a symptomatic benefit.
Apoptotic DNase network: Mutual induction and cooperation among apoptotic endonucleases
Authors: Basnakian Alexei G. , Moore Christopher L. 
Published: June 2021

AbstractDNA fragmentation produced by apoptotic DNases (endonucleases) leads to irreversible cell death. Although apoptotic DNases are simultaneously induced following toxic/oxidative cell injury and/or failed DNA repair, the study of DNases in apoptosis has generally been reductionist in approach, focusing on individual DNases rather than their possible cooperativity. Coordinated induction of DNases would require a mechanism of communication; however, mutual DNase induction or activation of DNases by enzymatic or non‐enzymatic mechanisms is not currently recognized. The evidence presented in this review suggests apoptotic DNases operate in a network in which members induce each other through the DNA breaks they produce. With DNA breaks being a common communicator among DNases, it would be logical to propose that DNA breaks from other sources such as oxidative DNA damage or actions of DNA repair endonucleases and DNA topoisomerases may also serve as triggers for a cooperative DNase feedback loop leading to elevated DNA fragmentation and subsequent cell death. Therefore, mutual induction of apoptotic DNases has serious implications for studies focused on activation or inhibition of specific DNases as a strategy for therapeutic intervention aimed at modulation of cell death.
A novel ferroptosis phenotype‐related clinical‐molecular prognostic signature for hepatocellular carcinoma
Authors: Deng Tuo , Hu Bingren , Jin Chen , Tong Yifan , Zhao Jungang , Shi Zhehao , Zhang Tan , Deng Liming , Sun Zhifu , Chen Gang , Wang Yi 
Published: June 2021

AbstractFerroptosis is a newly identified cell death mechanism and potential biomarker for hepatocellular carcinoma (HCC) therapy; however, its clinical relevance and underlying mechanism remain unclear. In this study, transcriptome and methylome data from 374 HCC cases were investigated for 41 ferroptosis‐related genes to identify ferroptosis activity‐associated subtypes. These subtypes were further investigated for associations with clinical and pathological variables, gene mutation landscapes, deregulated pathways and tumour microenvironmental immunity. A gene expression signature and predictive model were developed and validated using an additional 232 HCC cases from another independent cohort. Two distinct ferroptosis phenotypes (Ferroptosis‐H and Ferroptosis‐L) were identified according to ferroptosis gene expression and methylation in the patients with HCC. Patients with the Ferroptosis‐H had worse overall and disease‐specific survival, and the molecular subtypes were significantly associated with different clinical characteristics, mRNA expression patterns, tumour mutation profiles and microenvironmental immune status. Furthermore, a 15‐gene ferroptosis‐related prognostic model (FPM) for HCC was developed and validated which demonstrated accurate risk stratification ability. A nomogram included the FPM risk score, ECOG PS and hepatitis B status was developed for eventual clinical translation. Our results suggest that HCC subtypes defined by ferroptosis gene expression and methylation may be used to stratify patients for clinical decision‐making.
Identification and assessment of PLK1/2/3/4 in lung adenocarcinoma and lung squamous cell carcinoma: Evidence from methylation profile
Authors: Deng Sisi , Lu Xiaoli , Zhang Zhi , Meng Rui , Li Mi , Xia Shilin 
Published: June 2021

AbstractLung cancer is a very aggressive cancer characterized with molecular heterogeneities in different subtypes, including lung adenocarcinoma and lung squamous cell carcinoma. However, few related molecular signatures have been established for the treatment of lung cancer subtypes. Polo‐like kinase (PLK) family is a crucial regulator during cell division. Aberrant genetic and epigenetic alteration of PLK members plays a controversial role among different cancers. In this study, we performed an analysis of transcriptional and protein expression to identify overexpressed PLK1/4 and under‐expressed PLK2/3 in lung cancer subtypes. We then analysed biological function of PLKs and related genes. Besides, we estimated a correlation of PLKs with patient's genders and TP53 mutation in lung cancer. Higher PLK1/4 expression was significantly associated with male patient and TP53 mutant status, separately. Moreover, we carried out a methylation profile analysis including methylation level, DNA methyltransferases correlation and survival analysis of global methylation. Global methylation survival analysis showed that prognostic value of PLK1/2/4 methylation remained the same significant trend between two lung cancer subtypes, whereas prognostic value of PLK3 methylation lacked consistency. Taken together, these results provided instructive insights into a comprehensive evaluation for advanced therapeutic strategy based on epigenetic evidences.
Metformin protects against insulin resistance induced by high uric acid in cardiomyocytes via AMPK signalling pathways in vitro and in vivo
Authors: Jiao Zhenyu , Chen Yingqun , Xie Yang , Li Yanbing , Li Zhi 
Published: June 2021

AbstractHigh uric acid (HUA) is associated with insulin resistance (IR) in cardiomyocytes. We investigated whether metformin protects against HUA‐induced IR in cardiomyocytes. We exposed primary cardiomyocytes to HUA, and cellular glucose uptake was quantified by measuring the uptake of 2‐NBDG, a fluorescent glucose analog. Western blot was used to examine the levels of signalling protein. Membrane of glucose transporter type 4 (GLUT4) was analysed by immunofluorescence. We monitored the impact of metformin on HUA‐induced IR and in myocardial tissue of an acute hyperuricaemia mouse model established by potassium oxonate treatment. Treatment with metformin protected against HUA‐reduced glucose uptake induced by insulin in cardiomyocytes. HUA directly inhibited the phosphorylation of Akt and the translocation of GLUT4 induced by insulin, which was blocked by metformin. Metformin promoted phosphorylation of AMP‐activated protein kinase (AMPK) and restored the insulin‐stimulated glucose uptake in HUA‐induced IR cardiomyocytes. As a result of these effects, in a mouse model of acute hyperuricaemia, metformin improved insulin tolerance and glucose tolerance, accompanied by increased AMPK phosphorylation, Akt phosphorylation and translocation of GLUT4 in myocardial tissues. As expected, AICAR, another AMPK activator, had similar effects to metformin, demonstrating the important role of AMPK activation in protecting against IR induced by HUA in cardiomyocytes. Metformin protects against IR induced by HUA in cardiomyocytes and improves insulin tolerance and glucose tolerance in an acute hyperuricaemic mouse model, along with the activation of AMPK. Consequently, metformin may be an important potential new treatment strategy for hyperuricaemia‐related cardiovascular disease.
Cardiac metallothionein overexpression rescues diabetic cardiomyopathy in Akt2‐knockout mice
Authors: Huang Shan , Wang Jiqun , Men Hongbo , Tan Yi , Lin Qian , Gozal Evelyne , Zheng Yang , Cai Lu 
Published: June 2021

AbstractTo efficiently prevent diabetic cardiomyopathy (DCM), we have explored and confirmed that metallothionein (MT) prevents DCM by attenuating oxidative stress, and increasing expression of proteins associated with glucose metabolism. To determine whether Akt2 expression is critical to MT prevention of DCM, mice with either global Akt2 gene deletion (Akt2‐KO), or cardiomyocyte‐specific overexpressing MT gene (MT‐TG) or both combined (MT‐TG/Akt2‐KO) were used. Akt2‐KO mice exhibited symptoms of DCM (cardiac remodelling and dysfunction), and reduced expression of glycogen and glucose metabolism‐related proteins, despite an increase in total Akt (t‐Akt) phosphorylation. Cardiac MT overexpression in MT‐TG/Akt2‐KO mice prevented DCM and restored glucose metabolism‐related proteins expression and baseline t‐Akt phosphorylation. Furthermore, phosphorylation of ERK1/2 increased in the heart of MT‐TG/Akt2‐KO mice, compared with Akt2‐KO mice. As ERK1/2 has been implicated in the regulation of glucose transport and metabolism this increase could potentially underlie MT protective effect in MT‐TG/Akt2‐KO mice. Therefore, these results show that although our previous work has shown that MT preserving Akt2 activity is sufficient to prevent DCM, in the absence of Akt2 MT may stimulate alternative or downstream pathways protecting from DCM in a type 2 model of diabetes, and that this protection may be associated with the ERK activation pathway.
Mutation of the novel acetylation site at K414R of BECN1 is involved in adipocyte differentiation and lipolysis
Authors: Li Chengqian , Xu Jun , Yu Qing , Wang Ping , Dong Bingzi , Shen Liyan , Wang Qing , Li Shufa , Yang Ying , Deng Yujie 
Published: June 2021

AbstractBECN1, a protein essential for autophagy, is involved in adipocyte differentiation, lipolysis and insulin resistance. The discovery of new mechanisms for modifying BECN1 in adipocytes may provide novel therapeutic targets for obesity. This study aimed to investigate the impact of mutations at the acetylation sites of BECN1 on adipocyte differentiation and lipolysis. We found that Ace‐BECN1 levels were increased in 3T3‐L1 adipocyte differentiation and isoproterenol‐/TNF‐α‐stimulated lipolysis and in subcutaneous and visceral adipose tissues of high‐fat diet mice. K414 was identified as an acetylation site of BECN1, which affects the stability of the BECN1 protein. Mutation at K414 of BECN1 affected autophagy, differentiation and lipolysis in 3T3‐L1 adipocytes. These data indicated the potential of BECN1 K414 as a key molecule and a drug target for regulating autophagy and lipid metabolism in adipocytes.
Transcriptome wide analysis of long non‐coding RNA‐associated ceRNA regulatory circuits in psoriasis
Authors: Lin Jingxia , Li Xuefei , Zhang Fangfei , Zhu Lei , Chen Yongfeng 
Published: June 2021

AbstractLong non‐coding RNAs (lncRNAs) play critical roles in regulating immune‐associated diseases and chronic inflammatory disorders. Here, we found that lncRNAs involve in the pathogenesis of psoriasis through integrative analysis of RNA‐seq data sets from a psoriasis cohort. Then, lncRNA‐protein‐coding genes (PCGs) co‐expression network analysis demonstrated that lncRNAs extensively interact with IFN‐γ signalling pathway‐associated genes. Further, we validated 3 lncRNAs associate with IFN‐γ signalling pathway activation upon IFN‐γ stimulated in HaCaT cells, and loss of function experiments indicate their functional roles in the activation of inflammatory cytokine genes. Additionally, microRNA target screening analysis showed that lncRNAs may regulate JAK/STAT pathway activity through complete endogenous RNA (ceRNA) mechanism. Further experimental validation of PRKCQ‐AS1/STAT1/miR‐545‐5p regulatory circuitry showed that lncRNAs regulate the expression of JAK/STAT signalling pathway genes through competing for miR‐545‐5p. In summary, our results demonstrated that dysregulation of lncRNA‐JAK/STAT pathway axis promotes the inflammation level in psoriasis and thus provide potential therapeutic targets for psoriasis treatments.