bluuebunny/crossref_metadata_2025_split huggingface dataset:
Graphene Oxide and Polyethyleneimine Cooperative Construct Ionic Imprinted Cellulose Nanocrystals Aerogel for Selective Adsorption of Dy(III)
Authors: Zheng Xudong , Sun Wen , Li Ang , Wang Bin , Jiang Rong , Song Zhiqiang , Zhang Yuzhe , Li Zhongyu 
Published: September 2021

Abstract
Because of dysprosium's unique physical and chemical properties and limited supply, the price of rare earth dysprosium has been high in recent years. Therefore, the study of the method of high efficiency selective separation of dysprosium has the double value of scientific research and practical economy. In this paper, we used periodic cellulose nanocrystals as the basic structure, polyethylenimine and graphene oxide were introduced, combined with imprinting technology, to construct porous imprinted aerogel and use it for selective adsorption of Dy(III). The physical and chemical properties were characterized by SEM, TEM, FT-IR and TGA. It was proved that both polyethylenimine and graphene oxide were crosslinked effectively with cellulose nanocrystals. Adsorption experiments showed that the composite imprinted aerogel could selectively adsorb dysprosium effectively, and the maximum adsorption capacity for Dy(III) was 36.495 mg g− 1. The reproducibility experiment showed that aerogel had good regeneration ability. In conclusion, cellulose nanocrystals aerogel, which is environmentally friendly, efficient and repeatable, is expected to provide a new direction for the recovery of rare earth elements.
The Genomic Characteristics of RET Fusion Positive Tumors in Chinese Non-Small Cell Lung Cancer (NSCLC) Patients
Authors: Wu Guowu , Guo Longhua , Gu Yinfang , Huang Tanxiao , Liu Ming , Zou Xiaofang , Yang Bo , Huang Ping , Wen Chunling , Yi Lilan , Liao Wenting , Zhao Dongdong , Zhu Junlin , Zhang Xiaoni , Liu Yuanyuan , Yin Yan , Chen Shifu 
Published: September 2021

Abstract
Background: Approximately 1-2% of non-small cell lung cancer (NSCLC) patients harbor RET (rearranged during transfection) fusions. The oncogenic RET fusions could lead to constitutive kinase activation and oncogenesis.Methods: 1746 Chinese NSCLC patients were enrolled from in this study. Tumor tissues were collected during surgery, and were formalin fixed, paraffin-embedded (FFPE) and archived. Peripheral blood (PB) samples were also collected from each patient as control. In addition, we selected 17 of them for cfDNA NGS testing and 14 tumor samples for immunohistochemistry testing using PD-L1 rabbit monoclonal antibody, clones 28-8 (Abcam, Cambridge, UK).Results: Of the 1746 NSCLC cases, RET rearrangements were identified in 25 cases (1.43 %) with locally advanced or metastatic NSCLC, of which 20 (80%) were female. We found that 14 out of 25 patients had an KIF5B-RET fusion, with KIF5B exon15-RET exon12, KIF5B exon23-RET exon12, and KIF5B exon24-RET exon11 detected in 14, 3, and 1 patients respectively. We also identified one novel RET fusion partner PLCE1 and 4 intergenic-breakpoint fusions. Conclusion: In this study, using the hybrid capture based NGS (the next generation sequencing) techniques, we revealed the genomic profiling for the patients with RET fusion-positive NSCLC. To the best of our knowledge, this is the first study that exhibited the detailed breakpoints of Chinese NSCLC patients with RET rearrangement, and we found a novel new partner PLCE1. The results provided genomic information for patients with RET fusion which is significant for personalized clinical management in the era of precision medicine.
Intranasal Dexamethasone: a New Clinical Trial For The Control of Inflammation and Neuroinflammation in Covid-19 Patients
Authors: Caádenas Graciela , Espinosa Ana María , Chavez-Canales María , Jordán-Ríos Antonio , Malagon Daniel Anica , Márquez Manlio F. , Torres-Araujo Laura , Barajas-Campos Ricardo , Wong Rosa , Ramírez Luis , Ibet Karent , García Enrique , Rodriguez Mariana , Leyva Yoana , Hernández-Ruíz Joselin , Hernández-Mendel María , León-Hernández Mireya , Medina Karen , Sánchez Anahi , Hernández Sergio , Zeron Ignacia , Martínez-Cuazitl Adriana , Martínez Iván , Beltrán Eduardo , Figueroa Aldo , Hernández Patricia , Aguilar Rafael I , Murillo-Reyes Daniela , Río Luis R. Del , Alfaro-Bonilla Rogelio A. , Cruz Joselyn , Huerta Leonor , Fierro Nora , Hernández Marisela , Pérez-Tapia Mayra , Meneses Gabriela , Jung Helgi , Espíndola-Arriaga Erick , Rosas Gabriela , Olguin Roxana , Ortega Sandra , Chinney Alberto , Rosales Sergio , Hernández-Aceves Juan , Cervantes-Torres Jaquelynne , Besedovsky Hugo , Romano Marta C. , Bobes Raúl J , Soldevila Gloria , Lopez Juan , Fragoso Gladis , Laclette Juan P , Sciutto Edda , Rodríguez Anai Fuentes 
Published: September 2021

Abstract
COVID-19 has produced more than 176 million infected individuals and almost 3.2 million deaths worldwide. The infection results in a dysregulated systemic inflammation, multi-organ dysfunction, and critical illness. Cells of the central nervous system (CNS) are also affected triggering a dysregulated neuroinflammatory response.Low doses of glucocorticoids (GCs) orally or intravenously administered has been proved to reduce mortality of moderate and severe COVID-19 patients. However, low doses administered by those routes do not reach therapeutic levels in the CNS. In contrast, if dexamethasone is administered by the intranasal route can result in therapeutic doses in the CNS even at low doses of the GC. Methods: This is an approved multicentric randomized controlled protocol to compare the effectiveness of low doses of intranasal dexamethasone versus intravenous administered in adult moderate and severe COVID-19 patients. The protocol is conducted in five health institutions in Mexico City. A total of 120 patients will be randomized in two groups (intravenous vs intranasal) at 1:1 ratio, both groups will be treated with these dexamethasone schemes for 10 days. The primary outcome of the study will be clinical improvement, defined as a statistically significant higher reduction in the NEWS-2 score in intranasally versus intravenously dexamethasone treated patients. The second outcome will be the reduction in mortality during hospitalization. Conclusions: This protocol is currently undertaken to improve the efficacy of the standard therapeutic dexamethasone regimen for moderate and severe COVID-19 patients. Trial registration: ClinicalTrials.gov identifier: NCT04513184 Registered November 12, 2020 and was approved by COFEPRIS with identifier DI/20/407/04/36. People are currently being recruited.
The Appropriate Dose of Remimazolam Combined with Remifentanil in Anesthesia for Gastroscopy
Authors: Zhang Jianyou , Wang Maohua , Liu Fengxia , Guo Miao , Zhu Yue , Li Shitong 
Published: September 2021

Abstract
Background: The appropriate doses of remimazolam combined with remifentanil were investigated in anesthesia gastroscopy by observing the effect of two methods of administration in fixed doses and dosed by ideal weight on success rate and recover effect in painless gastroscopy. Methods: A total of 400 patients underwent the anesthesia of gastroscopy were randomized to divide into five groups (W1, W2, F1, F2 and C Group) in endoscopy center, with 80 cases in each group. All patients were given 0.25ug/kg of remifentanil before gastroscopy. The success rate, the quality of anesthesia resuscitation, heart rate, blood pressure, Pulse oxygen saturation, perioperative adverse events and Modified observer’ s assessment of alert/sedation (MOAA/S) diachronic changes were recorded. Results: In terms of anesthetic effects of the first dose of the drug(P<0.05), the patients in F1 group showed be lack of sedation obviously. The success rate of induction in F2 group was higher than that in F1 group. The success rate of sedation in W2 groups was highest (96%). The control group was second to W2 Group. Blood pressure in W2 Group was lower than W1 Group after first administration(P<0.05). There was no significant difference in next several minutes. There was no difference in blood pressure between W2 Group and C Group at T1(P>0.05). There was no difference in the changes of heart rate, recovery time and discharge time(P>0.05). The incidence of cough in F1 and W1 is high(P<0.05). Conclusions: Remimazolam combined with remifentanil used were safe, and 0.3mg/kg of remimazolam combined with 0.25ug/kg of remifentanil by ideal body weight is better in painless gastroscopy.Trial registration: The trial was registered on ClinicalTrials.gov: ChiCTR2100041759, registered 4 January 2021.
Prevalence and Associated Factors of Pre-Hypertension Among Adults Visiting the Outpatient Department at Mizan Tepi University Teaching Hospital, Mizan-Aman, Ethiopia, 2021.
Authors: Guteta Mirresa 
Published: September 2021

Abstract
Background: Prehypertension is a systolic blood pressure (SBP) of 120-139 millimeters of mercury (mmHg) and a diastolic blood pressure (DBP) of 80-89 millimeters of mercury (mmHg) According to JNC-7 report the new added category. It is considered as a starting point for cardiovascular and many other morbidities. Little is known about the magnitude of prehypertension and its associated factors in study area. Objective: To assess prevalence of Pre-hypertension and Associated factor among adults visiting the Outpatient department at Mizan Tepi university teaching hospital, Mizan-Aman, Ethiopia, 2021.Methods: Institution based cross sectional study design was conducted in Mizan Tepi University Teaching hospital. Simple random sampling method was used to recruit study participants. Data was entered into Epi data version 3.1 and exported to SPSS version 23 for data analysis. The descriptive analysis of data was done and the result presented using frequency tables and graphs. Multivariable logistic regression model was fitted, Adjusted Odds Ratio (AOR) at 95% confidence interval and p-value <0.05 were estimated to determine statistically significant association between predictors and outcome variable.Result: The prevalence of Prehypertension among adults visiting the Outpatient department at Mizan Tepi university teaching hospital was 30.7%. Significant association of prehypertension was found with age (AOR=1.22, 95% CI: 0.69, 2.15), educational status (AOR=0.32, 95% CI: 0.11, 1.00), physical exercise (AR=1.11, 95% CI: 0.57, 2.16) and Body Mass Index (AOR=4.14, 95% CI: 0.44, 39.34). Conclusion: Pre-Hypertension is high in the study area. As a result, concerned body should take action to enhance patients visiting Mizan Tepi University Teaching hospital about impact of Pre-Hypertension on individual health and how to control it.
Parentage Assignment Reveals Multiple Paternity in the Critically-Endangered Guatemalan Beaded Lizard (Heloderma Charlesbogerti)
Authors: Levine Brenna A , Hill Robert , Mendelson Joseph , Booth Warren 
Published: September 2021

Abstract
Within captive management programs for species of conservation concern, understanding the genetic mating system is of fundamental importance, given its role in generating and maintaining genetic diversity and promoting opportunities for sperm competition. If a goal of a conservation program is reintroduction, knowledge of the mating system may also inform prediction models aimed at understanding how genetic diversity may be spatially organized, thus informing decisions regarding where and which individuals should be released in order to maximize genetic diversity in the wild population. Within captive populations, such information may also influence how animals are maintained in order to promote natural behaviors. Here we investigate the genetic mating system of the Guatemalan beaded lizard, Heloderma charlesbogerti, a member of a genus lacking such information. A group of adult male and female H. charlesbogerti were co-habited for five years during the species perceived breeding season. Through genomic parentage analysis, 50% of clutches comprising multiple offspring were found to result from polyandry, with up to three males siring offspring within single clutches. Furthermore, males were found to be polygamous both within and across seasons, and females would exhibit promiscuity across seasons. As such, within this captive environment, where opportunities existed for mating with multiple sexual partners, the genetic mating system was found to be highly promiscuous, with multiple paternity common within clutches. These findings are novel for the family Helodermatidae, and the results have broader implications about how reproductive opportunities should be managed within captive conservation programs.
Dissections or Prosections; Which Method has a Better Impact on Sustainable Gross Anatomy Knowledge?
Authors: Wimalachandra Manujasri , Balagobi Balasingam , Hashintha Malith , Ranasinghe Narada , Niroshana Lamindu , Anthony Joseph , Jayasekara. Rohan 
Published: September 2021

Abstract
Background: Two groups of medical students in their final year were tested on the key concepts of gross anatomy using a question paper that included true false type questions and identification of anatomical line diagrams. These two batches of students followed a dissection based curriculum and a newly introduced prosections based curriculum at the beginning of their medical education respectively. The prosections based curriculum brought with it a significant cut down on the in-class teaching and learning activities when compared to the old curriculum. The objective of the study was to establish which method was more effective at establishing a core of anatomy knowledge that could be recalled after a considerable amount of time. This would in turn reflect how much of anatomy knowledge one would possess when they start to practice medicine as a newly qualified doctor and also embark on a postgraduate training programme.Methods: The two groups were subjected without prior warning to a question paper that comprised six questions, each with five True/False statements and four questions on identification and labelling of anatomical line diagrams.Results: There was no statistically significant difference in the marks obtained for the true false type questions between the two groups (p=0.076), but the prosections group obtained higher marks for the diagram identification questions (p = 0.022).Conclusions: Therefore, it was concluded that a prosection based curriculum when compared to a dissection based curriculum was equally effective at establishing a core of gross anatomy knowledge in a considerably lesser amount of time.
Quality of Nursing Education in the Philippines: The Impact of Nursing Programme Profile
Authors: Appiah Stella 
Published: September 2021

Abstract
Background: The production of nursing professionals who are capable of delivering quality health care services hinges on the quality of nursing education programme. Nevertheless, quality of nursing education programme has always been judged from the point of licensure exams results and calibre of faculty members. Objectives: To Investigate the impact of profile of nursing education programme such as years of establishment, kind of accreditation agency and level of accreditation on the quality of nursing education programme in the Philippines. Design: A cross-sectional survey study design was employed in this study. Study settings and Participants: One hundred and eight-five (185) faculty members in fifteen (15) higher educational institutions in the Philippines were selected for the research using purposive-census sampling. The study was carried out from January 1 to June 30, 2017. Method: Close-ended structured questionnaires based on study objectives were used to collect data. Frequency and percentages were used to analyse the profile of faculty members whereas weighted means from a four Likert’s scale was used to interpret the extent of perceived quality of nursing education programme.Results: Nursing education programmes offered in the Philippines was found to be of good quality. Years of establishment of nursing education programme showed significant impact on the quality of curriculum and instruction (p-value=0.007), physical structure and equipment (p-value=0.001), student services (p-value=0.001) and admission of nursing students (p-value=0.003) while level of accreditation had significant effect on the quality of administration nursing programme (p-value=0.000), faculty development programme (p-value=0.001), physical structure and equipment (p-value=0.000), student services (p-value=0.001), admission of student (p-value=0.006) and quality assurance system (p-value=0.000) of nursing education programme. However, accreditation agency only had significant impact on quality of admission of students (p-value=0.047).Conclusion: Profile of nursing education programme had significant impact on some criteria pertaining to the quality of nursing education programme in the Philippines.
Dental Students' Perception and Confidence Level in Key Dental Procedures for General Practice and the Impact of Competency Implementation On Their Confidence Level, Part I (Prosthodontics and Conservative Dentistry)
Authors: Elmanaseer Wijdan R. , Al-Omoush Salah , Alsoleihat Firas 
Published: September 2021

Abstract
Background: There are limited studies that assess the level of confidence among the final year dental students in the ability to conduct key dental procedures. The present study aims to assess the perception and confidence level of final year dental students ‎from the School of Dentistry at the University of Jordan to perform essential dental procedures ‎across various dental disciplines; to assess the effect of competencies implementation in curricula on the confidence level of students. Methods: An electronic questionnaire was answered by two cohorts of final year dental students: one group in 2016 before the implementation of the competency-based assessment system (group 1, n = 153), and the other in 2019 after the implementation of this system (group 2, n = 199). The two groups were compared regarding the degree of confidence in conducting key dental procedures. The data were analysed using SPSS statistics. Statistical significance was set at 0.05 level.Results: Statistically significant differences between the two groups regarding the confidence levels were found in 5 out of 20 prosthodontic procedures (placing treatment plan of removable partial dentures, using semi-adjustable articulator, giving oral hygiene instructions (OHIs) for denture patients, dealing with complete denture post-insertion complaints, and giving OHIs for denture patients); and in 5 out of 20 conservative Dentistry procedures (placing treatment plan of removable partial dentures (RPD), using semi-adjustable articulator, giving OHI's for Denture Patients, dealing with complete denture (CD) post-insertion complaints and giving OHI's for Denture patients). These differences were in favor of grope 2.Conclusion: This study has shown that final year dental students generally have high confidence levels in doing simple dental procedures and less confidence in more complex ones, thus additional focus on such complex procedures is advised during post-graduation training and before entitlement for practice licensing. Competency-based dental education is an advantageous over classical dental training systems, and thus should be maintained and continuously enhanced.
First-Degree Relationships and Genotyping Errors Deciphered by a High-Density SNP Array in a Duroc × Iberian Pig Cross
Authors: Gomez-Raya Luis , Izquierdo Emilio , Peña Eduardo Mercado de la , Garcia-Ruiz Fabian , Rauw Wendy Mercedes 
Published: September 2021

Abstract
Background Two individuals with a first-degree relationship share about 50 percent of their alleles. Parent-offspring relationships cannot be homozygotes for alternative alleles (genetic exclusion). Methods Applying the concept of genetic exclusion to HD arrays typed in animals for experimental purposes or genomic selection allows estimation of the rate of rejection of first-degree relationships as the rate at which two individuals typed for a large number of SNPs do not share at least one allele. An Expectation–Maximization algorithm is applied to estimate parentage. In addition, genotyping errors are estimated in true parent-offspring relationships due to the large number of SNPs. Nine candidate Duroc sires and 55 Iberian dams producing 214 Duroc × Iberian barrows were typed for the HD porcine Affymetrix array. Results We were able to establish paternity and maternity of 75 and 86 piglets, respectively. A lower bound of the genotyping error of 0.003345 was estimated based on the rate of rejection of true parent-offspring relationships among autosomal SNPs. The true genotyping error is estimated to be between twice and three times the average of the rate of rejection observed in true relationships, i.e., between approximately 0.0067 and 0.0100. A total of 8,558 SNPs were rejected in six or more true parent-offspring relationships facilitating identification of “problematic” SNPs with inconsistent inheritance. Conclusions This study shows that animal experiments and routine genotyping in genomic selection allow to establish or to verify first-degree relationships as well as to estimate genotyping errors for each batch of animals or experiment.
JQ1 Attenuates Neuroinflammation by Inhibiting Inflammasome-Dependent Canonical Pyroptosis Pathway in Sepsis-Associated Encephalopathy
Authors: Zhong Xiaolin , Chen Zuyao , Wang Yajuan , Mao Mingli , Deng Yingcheng , Shi Mengmeng , Xu Yang , Chen Ling , Cao Wenyu 
Published: September 2021

Abstract
Sepsis-associated encephalopathy (SAE) is manifested clinically in hyperneuroinflammation and associated with increased morbidity worldwide. Pyroptosis, a novel programmed cell death, has been considered as a causative factor of SAE. Bromodomain-containing protein 4 (BRD4) is a member of the Bromo and Extra-Terminal (BET) family, and promotes inflammatory response in various diseases. Thereby, we examined the effect of JQ1, which is a specific selective inhibitor of BRD4, on the inflammasome-induced pyroptosis of hippocampus in sepsis mice model induced by lipopolysaccharide (LPS) treatment. And we found that JQ1 treatment inhibited the phosphorylation of BRD4, alleviated weight loss and splenomegaly, as well as decreased the serum levels of procalcitonin (PCT) and D-lactate dehydrogenase (D-LDH) induced by LPS injection. Moreover, JQ1 administration decreased the expression of nod-like receptor family protein 1 (NLRP1) or 3 (NLRP3) or the absent in melanoma 2 (Aim2) inflammasomes by blocking nuclear factor kappa B (NFκB) signaling in the hippocampus of sepsis mice. Interestingly, we found that JQ1 selectively attenuated the canonical pyroptosis pathway in SAE mice, indicating by reduced expression of Caspase-1, Caspase-11, gasdermin D (GSDMD) and gasdermin A (GSDMA). JQ1 intervention also suppressed the activation of hippocampal microglia and the release of pro-inflammatory factors, such as IL-1β, IL-18 and IL-6 in SAE mice. In addition, JQ1 treatment protected blood brain barrier (BBB) by up-regulated the expression of tight junction protein occludin and ZO-1 in SAE mice. Furthermore, JQ1 administration remarkably rescued neuronal damage in SAE mice, as enhanced expression of hippocampal NeuN and Doublecortin (DCX). Thus, the protective effects of BRD4 inhibitor JQ1 on SAE were verified in neurons via the inhibition of canonical pyroptosis induced inflammation.