bluuebunny/crossref_metadata_2025_split huggingface dataset:
Multi-omics approaches reveal that diffuse midline gliomas present altered DNA replication and are susceptible to replication stress therapy
Authors: Hains Anastasia E. , Chetal Kashish , Nakatani Tsunetoshi , Marques Joana G. , Ettinger Andreas , Junior Carlos A. O. Biagi , Gonzalez-Sandoval Adriana , Pillai Renjitha , Filbin Mariella G. , Torres-Padilla Maria-Elena , Sadreyev Ruslan I. , Van Rechem Capucine 
Published: December 2024

Abstract

Background
The fatal diffuse midline gliomas (DMG) are characterized by an undruggable H3K27M mutation in H3.1 or H3.3. K27M impairs normal development by stalling differentiation. The identification of targetable pathways remains very poorly explored. Toward this goal, we undertake a multi-omics approach to evaluate replication timing profiles, transcriptomics, and cell cycle features in DMG cells from both H3.1K27M and H3.3K27M subgroups and perform a comparative, integrative data analysis with healthy brain tissue.

Results
DMG cells present differential replication timing in each subgroup, which, in turn, correlates with significant differential gene expression. Differentially expressed genes in S phase are involved in various pathways related to DNA replication. We detect increased expression of DNA replication genes earlier in the cell cycle in DMG cell lines compared to normal brain cells. Furthermore, the distance between origins of replication in DMG cells is smaller than in normal brain cells and their fork speed is slower, a read-out of replication stress. Consistent with these findings, DMG tumors present high replication stress signatures in comparison to normal brain cells. Finally, DMG cells are specifically sensitive to replication stress therapy.

Conclusions
This whole genome multi-omics approach provides insights into the cell cycle regulation of DMG via the H3K27M mutations and establishes a pharmacologic vulnerability in DNA replication, which resolves a potentially novel therapeutic strategy for this non-curable disease.
The inactivation of the Niemann Pick C1 cholesterol transporter restricts SARS-CoV-2 entry into host cells by decreasing ACE2 abundance at the plasma membrane
Authors: La Rosa Piergiorgio , Tiberi Jessica , Palermo Enrico , Stefanelli Roberta , Tiano Sofia Maria Luigia , Canterini Sonia , Cortese Mirko , Hiscott John , Fiorenza Maria Teresa 
Published: December 2024

Abstract
Background
The Niemann Pick C1 (NPC1) protein is an intracellular cholesterol transporter located in the late endosome/lysosome (LE/Ly) that is involved in the mobilization of endocytosed cholesterol. Loss-of-function mutations in the NPC1 gene lead to the accumulation of cholesterol and sphingolipids in LE/Ly, resulting in severe fatal NPC1 disease. Cellular alterations associated with NPC1 inactivation affect both the integrity of lipid rafts and the endocytic pathway. Because the angiotensin-converting enzyme 2 (ACE2) and type 2 serine transmembrane protease (TMPRSS2), interactors of the SARS-CoV-2 Spike protein also localize to lipid rafts, we sought to investigate the hypothesis that NPC1 inactivation would generate an intrinsically unfavorable barrier to SARS-CoV-2 entry.

Results
In this study, we show that inhibition of the cholesterol transporter activity of NPC1 in cells that express both ACE2 and TMPRSS2, considerably reduces SARS-CoV-2 infectivity, evaluated as early as 4 h post-infection. Mechanistically, treatment with NPC1 specific inhibitor U18666A relocalizes ACE2 from the plasma membrane to the autophagosomal/lysosomal compartment, thereby reducing SARS-CoV-2 entry into treated cells. Reduction of viral entry was observed for both fully infectious SARS-CoV-2 virus and with a pseudotyped VSV-Spike-GFP virus. For instance, U18666A-treated Caco-2 cells infected with the pseudotyped VSV-Spike-GFP showed a > threefold and > 40-fold reduction in virus titer when infectivity was measured at 4 h or 24 h post-infection, respectively. A similar effect was observed in CRISP/R-Cas9-edited Caco-2 cells, which were even more resistant to SARS-CoV-2 infection as indicated by a 97% reduction of viral titers.

Conclusion
Overall, this study provides compelling evidence that the inhibition of NPC1 cholesterol transporter activity generates a cellular environment that hinders SARS-CoV-2 entry. ACE2 depletion from the plasma membrane appears to play a major role as limiting factor for viral entry.
Assessment of compliance and therapeutic efficacy of albendazole treatment in Chinese patients with echinococcosis
Authors: Qin Min , Yang Guobing , Yan Jun , Wang Liying , Feng Yu , Wang Dong , Wang Qian , Hou Yanyan , Zhao Jiangshan , Lei Jiaxi , Wang Zhiyi , Jiang Mingzhe , Yu Chenghang , Gavotte Laurent , Frutos Roger 
Published: December 2024

Abstract
Background
Echinococcosis is an infectious parasitic disease that is extremely harmful to human health. Albendazole is provided free of charge to patients requiring medication under the central government finance transfer payment scheme for echinococcosis control and prevention in China. Our aim is to monitor the state of patient medication and its therapeutic impact, which will help improve medication compliance and the therapeutic effect.

Methods
Random cluster sampling was used to select 10 echinococcosis-endemic counties in China, and all albendazole-treated patients in these counties were investigated. The chi-square and Kruskal–Wallis tests were used to compare two or more rates or constituent ratios, and multiple logistic regression analysis was used to identify the influencing factors. The records of patients were reviewed to obtain the initial diagnosis results as well as the most recent follow-up results and time, and efficacy was assessed.

Results
We examined 899 patient files treated with albendazole in 10 endemic counties. Of the 582 evaluable files, 7.9% did not take albendazole, and 69.2% did not take albendazole regularly. Only 22.9% took albendazole regularly. Of the 536 patients who took albendazole, 242 exhibited adverse reactions. Patients who were Tibetan, herdsmen, received no formal education, used emulsion, and exhibited adverse reactions demonstrated poor compliance. A total of 174 patients with cystic echinococcosis received their most recent imaging follow-up results within one year of the investigation date. Among them, 9 patients met the criteria for cure, accounting for 5.2%; 56 patients showed effectiveness, accounting for 32.2%; 105 patients were deemed ineffective, accounting for 59.8%; 5 patients experienced recurrence, accounting for 2.9%.

Conclusions
Albendazole medication compliance in patients with echinococcosis is not ideal. We must prioritize health education and promotion for Tibetans, herdsmen, and those without formal education. Patients who adhered to their medication regimen achieved higher rates of cure and effectiveness. To improve medication compliance and efficacy, it is particularly important to improve communication and medication guidance for patients receiving emulsions and those with adverse reactions after taking albendazole. Simultaneously strengthen patients' attention to follow-up and re-examination.

Graphical Abstract
Ecological trade-offs associated with fuel breaks in sagebrush ecosystems
Authors: Roche Morgan D. , Saher D. Joanne , Buchholtz Erin K. , Crist Michele R. , Shinneman Douglas J. , Aldridge Cameron L. , Brussee Brianne E. , Coates Peter S. , Weise Cali L. , Heinrichs Julie A. 
Published: December 2024

Abstract
Background
Unprecedented wildfire frequency, fueled by invasive annual grasses, threatens sagebrush ecosystems. To suppress wildfire and conserve sagebrush, land management agencies have installed fuel breaks across the sagebrush biome. However, despite the potential reduction in wildfire, fuel breaks may have ecological costs. Determining an acceptable balance between risks and benefits of fuel breaks is needed to avoid accelerating sagebrush loss, annual grass invasion, and habitat degradation. To evaluate the potential for ecological trade-offs to occur, we characterized the contexts in which known fuel breaks currently exist. We synthesized spatial data on all known fuel breaks and a suite of variables that may contribute to fuel break risks and benefits, including burn probabilities, predicted fuel break effectiveness, linear infrastructure, invasive annual grass cover, soil moisture conditions that confer resistance to invasion and resilience to disturbance, and priority wildlife habitats across the sagebrush biome.

Results
We found that within the sagebrush biome, fuel breaks are generally located in areas with high burn probability and are thus positioned well to intercept potential wildfires. However, fuel breaks are also frequently positioned in areas with lower predicted fuel break effectiveness relative to the sagebrush biome overall. Fuel breaks also are spatially associated with high invasive grass cover, indicating the need to better understand the causal relationship between fuel breaks and annual invasive grasses. We also show that the fuel break network is dense within priority wildlife habitats. Dense fuel breaks within wildlife habitats may trade off wildfire protection for decreased integrity of such habitats.

Conclusions
Our analyses describe the potential for fuel breaks to invoke ecological trade-offs and show that the balance of risks and benefits differs across sagebrush ecosystems. Strategic research and actions are needed to evaluate which factors tip the balance towards maximizing wildfire suppression while minimizing risk to sensitive ecological resources.
Literary Meat Works
Authors: Singer Hayley 
Published: December 2024

Abstract
In Agustina Bazterrica's novel Tender Is the Flesh (2020), a virus has ripped through every animal on the planet. All animals are massacred. No more dogs, cows, pigs, or birds. Some people refused to send their companions to the killing squads. Still, they died. At first, “expendable” humans were sold as black-market substitutes for animal protein, but now cannibalism has been legitimized—though it is, in fact, illegal to use the correct terms for what goes on in the Municipal Slaughterhouse. This article looks to Tender Is the Flesh to understand how language and narrative form can be worked in ways that articulate and “unwrite” multispecies injustices. The article argues for the value of literature that responds to culturally suppressed realities of abattoirs by imagining how it might feel to be subjected to these realities. The question whether such imaginative work can expand the possibilities of multispecies justice is examined.
The Effect of Motor Imagery Training on Lower Limb Function in Older Adults: A Systematic Review
Authors: Zhang Mufan , Zhang Tingran , Zhang Jiahan , Luo Jiong 
Published: December 2024

Background Population aging is a significant trend in China's development, and the decline in lower limb function in older adults is one of the primary health threats, often leading to falls or injuries. Several studies have applied motor imagery (MI) to improve lower limb function in older adults. However, the benefits of this intervention method for older adults remain to be further explored. Objective This paper systematically reviews the benefits of motor imagery in improving lower limb function in older adults using the International Classification of Functioning, Disability, and Health (ICF) framework. Methods Relevant literature on motor imagery interventions targeting balance ability in healthy older adults was retrieved from four English and three Chinese databases. The content of the literature was analyzed and coded based on the theoretical framework of the ICF. Results A total of 11 studies from 7 countries were included, comprising 11 randomized controlled trials (RCTs) with 758 older adult participants. Interventions included MI alone or combined with physical exercise, and intervention settings encompassed laboratories, communities, hospitals, and homes. Outcome measures included motor function, postural changes and maintenance, gait, and mobility. Conclusion Motor imagery interventions and their combination with other intervention methods can improve lower limb function and related motor abilities in older adults. However, compared to MI alone, the effects of combined MI interventions on lower limb function in older adults remain inconclusive.
Predictive factors for the diagnosis of coeliac disease in children and young people in primary care: A systematic review and meta-analysis
Authors: Farrier Christian E. , Wanat Marta , Harnden Anthony , Paterson Amy , Roberts Nia , Saatci Defne , Hirst Jennifer 
Published: December 2024

Background
Coeliac Disease (CD) often has its onset in childhood and affects 1% of the population. This review aimed to identify important predictive factors for coeliac disease in children and young people which could help GPs decide when to offer testing.

Methods
We searched MEDLINE, Embase and Cochrane Library to April 2024. Included studies were observational or randomized trials reporting the risk of CD when exposed to predictive factor(s) in people ≤25 years of age. Genetic factors were excluded. Risk of Bias was assessed using the Newcastle-Ottawa Scale. Random effects meta-analysis was performed for factors reported in ≥5 studies to calculate pooled odds ratios (OR) or standardized mean differences (SMD).

Results
Of 11,623 unique abstracts, 183 were included reporting on 140+ potentially associated factors. Meta-analyses of 28 factors found 14 significant associations with CD diagnosis: having type 1 diabetes (OR 8.70), having a first degree relative with coeliac disease (OR 5.19), being of white ethnicity (OR 2.56), having thyroid disease (OR 2.16), being female (OR 1.53), more frequent gastroenteritis in early childhood (OR 1.48), having frequent respiratory infections in early childhood (OR 1.47), more gluten ingestion in early life (OR 1.25), having more infections in early life (OR 1.22), antibiotic use in early childhood (OR 1.21), being born in the summer (OR 1.09), breastfeeding (OR 0.79) older age at diagnosis of type 1 diabetes (OR 0.64), and heavier weight (SMD -0.21). The final three were associated with lower risk of CD diagnosis.

Discussion
This is the first systematic review and meta-analysis of predictive factors for CD in children. Amongst the 14 factors we identified that were significant, three were potentially modifiable: breast feeding, antibiotic use and amount of gluten ingestion in early childhood. This work could inform the development of clinical support tools to facilitate the early diagnosis of CD.
The prevalence of anemia and the factors associated with its severity among children aged 6–59 months in Ghana: A multi-level ordinal logistic regression
Authors: Asgedom Yordanos Sisay , Habte Aklilu , Woldegeorgis Beshada Zerfu , Koyira Mengistu Meske , Kedida Beimnet Desalegn , Fente Bezawit Melak , Gebrekidan Amanuel Yosef , Kassie Gizachew Ambaw 
Published: December 2024

Introduction
Anemia is a significant health problem that has a profound impact on young children under the age of five. It can result in severe consequences, such as stunted growth, impaired cognitive and motor development, increased illness, and even death. In Ghana, anemia is the leading cause of child mortality, yet there is a lack of information available on the prevalence of anemia and the factors associated with its severity in children under five in the country. To fill this gap, this study was conducted to investigate the prevalence and determinants of anemia severity among children aged 6–59 months in Ghana.

Methods
This study used data from the 2022 Ghana Demographic and Health Survey (GDHS) and included a weighted total sample of 3585 children aged 6–59 months. Given the hierarchical nature of the DHS data and the ordinal nature of anemia, a multilevel ordinal logistic regression model was employed. The Brant test was used to determine whether the proportional odds assumption was met (P ≥0.05). Deviance was used for model comparison. For the multivariable analysis, variables with a p-value ≤0.2 in the bi-variable analysis were considered. The Adjusted Odds Ratio (AOR) with a 95% Confidence Interval (CI) was reported as a factor associated with anemia severity in the multivariable multilevel proportional odds model.

Results
A study conducted in Ghanaian children aged 6–59 months found that 49.1% of them had anemia (95% CI: 47.4%-50.7%). The results indicated that 27.6% of patients had mild anemia, while 21.4% had moderate to severe anemia. Factors that were significantly associated with higher odds of childhood anemia included being 6–23 months old, male, having a maternal age of 15–24 or 25–34 years, belonging to poorer or wealthier households, having a higher birth order, being stunted, having maternal anemia, no media exposure, and living in the Northern, Upper East, or Upper West regions.

Conclusion
Anemia among children aged 6–59 months in Ghana is a major public health concern. It is recommended to improve access to the media, address maternal anemia through targeted interventions, and strengthen the wealth status of families. Furthermore, preconception care for mothers during pregnancy should be supported to reduce anemia in the long-term. Additionally, the early detection and management of stunted children should be strengthened to decrease childhood anemia.
Optimizing FSO systems for 6G network using particle swarm optimization
Authors: Allaoua Oumaima , Djellab Hanane , Maamri Fouzia , Belhocine Yacine , Saidi Riad , Boumehrez Farouk 
Published: December 2024

Abstract
Free Space Optics (FSO) is an innovative optical communication technology that leverages light propagation in free space for direct line-of-sight interactions. This technology is characterized by several benefits, including secure transmission, high bandwidth, and rapid data transfer rates, making it a promising candidate for integration into emerging 6G networks. However, the efficiency of FSO systems is adversely affected by various weather conditions, such as rain, temperature fluctuations, snow, and fog, as well as atmospheric disturbances. This paper presents a Particle Swarm Optimization (PSO)-based approach to optimize the divergence angle of FSO systems. PSO is a type of artificial intelligence that excels at managing complex settings and adapting to changes in the environment, thus making FSO systems more robust against such challenges. By optimizing this critical parameter, the proposed method significantly improves the reliability and efficiency of data transmission in FSO systems, supporting the advanced performance demands of future 6G networks. This optimization ensures FSO systems can achieve faster and more reliable connectivity, essential for the next generation of wireless communication.
Welterbe und Immaterielles Erbe – Was verbindet es und was unterscheidet es?
Authors: Albert Marie-Theres 
Published: December 2024

Abstract
Das Erbe schafft und prägt Identität, bietet Sicherheit und trägt zur Nachhaltigkeit bei. In diesem Zusammenhang wird das vielfältige materielle und immaterielle Erbe der Welt als Ergebnis menschlicher Kreativität anerkannt, das für eine nachhaltige Entwicklung genutzt werden sollte. Der Schutz des Erbes der Menschheit ist daher eine Herausforderung für die Zivilgesellschaft und liegt in der Verantwortung der internationalen Gemeinschaft. Als Reaktion auf diese Herausforderung hat die internationale Gemeinschaft einerseits das „Übereinkommen zum Schutz des Kultur- und Naturerbes der Welt“ und andererseits das „Übereinkommen zur Bewahrung des immateriellen Kulturerbes“ verabschiedet und umgesetzt. In diesem Artikel wird erörtert und reflektiert, wie diese Übereinkommen trotz ihrer unterschiedlichen Ziele und Vorgaben in ihrem übergreifenden Potenzial für den Aufbau und Schutz von Identität und die Förderung von Nachhaltigkeit zusammenlaufen.
Moderner Tanz in Deutschland
Authors: Kämpfe Vicky 
Published: December 2024

Abstract
2022 wurde die Praxis des Modernen Tanzes in Deutschland in das Internationale Verzeichnis des Immateriellen Kulturerbes der UNESCO aufgenommen. Der Beitrag verfolgt die Impulse und Entwicklungen dieser Praxis und hebt ihre Hauptcharakteristiken und sozialen Funktionen hervor. Ferner untersucht er die Probleme, die aus der Abstimmung des Schutzes traditionellen Wissens mit seiner kreativen Weiterentwicklung in der heutigen Ausübung tänzerischer Traditionen entstehen können. Abschließend folgt eine Analyse der gegenwärtigen Herausforderungen und der Perspektiven eines lebendigen Erbes der Praxis des Modernen Tanzes.
Es ging in den ersten Jahrzehnten des 20. Jahrhunderts darum, aus der Gegenwärtigkeit der Körper eine lebenswerte, eine bessere Zukunft zu schlagen wie humanistische Funken aus dem Feuerstein.
Ritter/Cramer in Odenthal 2019, S. 255f.
Renewing Museum Meanings and Action with Intangible Cultural Heritage
Authors: Stefano Michelle L. 
Published: December 2024

Abstract
On August 24, 2022, the International Council of Museums (ICOM) adopted a new Museum Definition after a multi-year process of membership consultation and debate. During its development, ‘diversity,’ ‘inclusivity,’ and ‘community participation,’ alongside heightened attention to ethics, emerged as key concepts that ought to shape understandings of contemporary museum functions and activity. In this light, I discuss the benefits of prioritizing intangible cultural heritage (ICH) in museum work, reflecting the values that have driven the process of redefinition, and that museum professionals strive to espouse. Against the current backdrop of mounting political, economic, sociocultural, and ecological challenges, I argue that raising ICH to a higher level of museological priority reframes museum approaches to community engagement with ethics and equity at the fore, where culture keepers are centered as the authorities of their living heritage through collaboration. In turn, fostering greater inclusivity and diverse community involvement in museological processes and programming can be achieved in more meaningful and lasting ways.
Empfehlungen zum künftigen Umgang mit dem Immateriellen Kulturerbe in Deutschland
Authors: Hanke Benjamin 
Published: December 2024

Abstract
Deutschland ist seit nunmehr elf Jahren Vertragspartei der UNESCO-Konvention zum Immateriellen Kulturerbe. Aus den gewonnenen Erfahrungen kann man Schlüsse für Kultur-, Bildungs- und Gesellschaftspolitik ziehen, die ermöglichen würden, das mit der nationalen Umsetzung verbundene Potential in allen drei Bereichen noch stärker zu nutzen. Der Beitrag geht Fragen in Bezug auf Kulturförderung, kulturpolitische Diskurse, Bildungsprozesse, gesellschaftliche Debatten und die Förderung einer „Kultur des Friedens“ durch Immaterielles Kulturerbe auf den Grund.
Magnetic metal oxide assisted conducting polymer nanocomposites as eco-friendly electrode materials for supercapacitor applications: a review
Authors: Ahmer Mohammad Faraz , Ullah Qasim , Uddin Mohammad Kashif 
Published: December 2024

Abstract
Magnetic metallic embedded conducting polymeric nanostructures have demonstrated a pivotal role in high-performance supercapacitors. Numerous research studies have attempted to develop new hybrid electrode materials through the incorporation of electronically conductive polymers into magnetic metallic oxides. To further enhance the electrochemical performance of conducting polymer (CP)–magnetic metal oxide (MMO) hybrid nanocomposites, an additional component (carbon-based material) has been mostly introduced into it. The focus of this review has been on highlighting the electrochemical importance of CP–MMO nanostructured composites in supercapacitor applications. The hybrid nanocomposite materials resulting from embedding conjugated polymers [polyaniline, polypyrrole, and poly (3,4-ethylenedioxythiophene)] and MMO (MnO2, NiO, and Co3O4) have been taken into consideration for discussion from most recent literature covering the period 2018–2024. The concise information presented in this review article will create awareness among researchers about the latest developments in the field of green energy-storing devices, particularly in the case of supercapacitors.