bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Presentation of Clinical Results of Radiolabelling of 99mTc-MIBI Complex Using Ultrasound Technique for Myocardial Perfusion Scanning (MPS)
Authors: Hassanpour Seyyed Hossein , Karami Seyyedeh Zeinab 
Published: February 2021

Abstract
Background
The initial objective and opinion of this study is introducing the clinical results of the 99mTc-MIBI complex radiolabelling utilizing ultrasound method as an approach for labeling MIBI kits with 99mTc instead of applying a boiling water bath as a usual and standard method.
Materials and methods
Twenty MIBI kits were bought for twenty patient volunteers. 555–3330 MBq (15–90 mCi) freshly eluted solution of Na+ 99mTcO4− was added to freeze-dry MIBI Kits. Moreover, ten kits were labeled applying ultrasound radiation method as an alternative approach (The vials were sonicated in thermo noted bath (Elma, P = 50 W, Germany) at 60\(\text{℃}\) and for 60 seconds), and other kits were provided applying boiling water bath method as a standard approach. Twenty patient volunteers (8 men and 12 women; age range 30–72, median 52.45 years) took part in this research. These people had gone to the hospital for myocardial perfusion imaging. These twenty volunteer patients were divided into two groups (every group contains ten people): group A (involving 3 men and 7 women with an age range from 36 to 67 years and a mean age of 51.7 years), and group B (involving 5 men and 5 women with an age range from 30 to 72 years and an average age of 50.3 years). The 99mTc-MIBI complex samples provided by ultrasound method were injected into group A subjects intravenously and group B received 99mTc-MIBI vials provided by boiling water bath approach (intravenous injection). In this research, the myocardial perfusion-SPECT was handled applying a rest-stress protocol which is done two isolated days for all volunteer patients.
Results
The results provided in this research applying radio-HPLC and ITLC, indicate that radio-labeling of MIBI vial with 99mTc can be provided with high effectiveness and appropriate acceptance applying ultrasound method as a novel approach. The imaging research applying SPECT indicated that the 99mTc-MIBI complex samples that were made ready applying ultrasound irradiation approach indicate very similar biodistribution in the heart, thyroid, lung, liver, gallbladder, kidneys, stomach, GI and bladder of the volunteer patients. Non-habitual aggregation of 99mTc-MIBI samples were not observed in our approach.
Conclusions
This research indicated that sonication approach can be proposed for providing 99mTc-MIBI radio-complex samples. The reaction time to provide radio-complex can be considerably decreased compared to the boiling water bath approach. This main development may decrease potential risk to the patient to inhibit any delay in emergency situations like acute therapy, especially for myocardial infarction patients in clinical practice.
Transcriptome and De Novo Analysis of Rosa xanthina f. spontanea&nbsp;in Response to Cold Stress
Authors: Zhuang Defeng , Ma Ce , Xue Li , Li Zhen , Wang Cheng , Lei Jiajun , Yuan Xingfu 
Published: February 2021

Abstract
Background: Rose is one of most popular ornamental plants all over the world, it is of high economical value and great cultural importance. However, chilling damage restricts its application in cold area. To elucidate the metabolic response under low temperature stress, we conducted transcriptome and De novo analysis in Rosa xanthina f. spontanea.Results: A total of 124,106 unigenes from 9 databases were generated by De novo assembly, with mean length of 661bp under 4℃ and -20℃ stress (23℃ as control). Functional annotation and prediction of 55,084 unigenes were detected and 67.72% of these unigenes had significant similarity (Blast, E≤10-5) in the public databases. 468 DEGs were involved in three groups: biological process (64.84%), cellular component (9.38%) and molecular function (25.78%), KEGG analysis suggested that metabolic pathway, response to plant pathogen interaction, starch and sucrose metabolism, circadian rhythm plant and photosynthesis antenna proteins were significantly enriched under cold stress.Conclusions: Our study first reported response to cold stress at the transcriptome level in Rosa xanthina f. spontanea, which can provides a theoretical basis for further studies on the molecular mechanism of cold-resistance in rose. The results was shown that the expression trend of eight DEGs selected by qRT-PCR analysis at random was consistent with the results of high-throughput sequencing.
PGE2 Displays Immunosuppressive Effects During Human Active Tuberculosis
Authors: Pellegrini Joaquín , Martin Candela , Morelli María Paula , Schander Julieta Aylen , Tateosian Nancy Liliana , Amiano Nicolás Oscar , Rollandelli Agustín , Palmero Domingo Juan , Levi Alberto , Ciallella Lorena , Colombo María Isabel , García Verónica Edith 
Published: February 2021

Abstract
Prostaglandin E2 (PGE2), an active lipid compound derived from arachidonic acid, regulates different stages of the immune response of the host during several pathologies such as chronic infections or cancer. In fact, manipulation of PGE2 levels was proposed as an approach for countering the Type I IFN signature of tuberculosis (TB). However, very limited information regarding the PGE2 pathway in patients with active TB is currently available. In the present work, we demonstrated that PGE2 exerts a potent immunosuppressive action during the immune response of the human host against Mycobacterium tuberculosis (Mtb) infection. Actually, we showed that PGE2 significantly reduced lymphoproliferation, the production of proinflammatory cytokines, and the surface expression of several immunological receptors. On the other hand, PGE2 promoted autophagy in monocytes and neutrophils cultured with Mtb antigens. These results suggest that PGE2 might be attenuating the excessive inflammatory immune response caused by Mtb, emerging as an attractive therapeutic target. Taken together, our findings contribute to the knowledge of the role of PGE2 in the human host resistance to Mtb and highlight the potential of this lipid mediator as a tool to improve anti-TB treatment.
Synthesis, Characterization and Antibacterial Activity of&nbsp;Novel&nbsp;β-Cyclodextrin Polyurethane Materials
Authors: Chen YueXing , Yan Jun , Zhang Yubin , Chen Wenwen , Wang Zefeng , Wang Lingnan 
Published: February 2021

Abstract
The advanced water treatment taken by organic micro-pollution or microbiological pollution water resource has been a hot issue of public concern. In this article, a novel quaternary ammonium salt functionalized β-cyclodextrin polyurethane (QAS-β-CDPU) was successfully constructed for above-mentioned pollutants removal. By adjusting the proportion of the chlorine-containing monomers, we studied the effect of different quaternization degree (0%, 20%, 40%, 60%) on solvent resistance and thermal stability. Staphylococcus aureus are recognized as significant human bacterial pathogens, which were used as model bacteria to investigate the antibacterial activity by contact-killing tests. Methylene blue (MB) served as a toxic organic model to quantify the dye wastewater remediation efficiency. Research shows that the reaction of β-cyclodextrin and diisocyanate can form a cyclodextrin-based carbamate network structure. Since both the cyclodextrin cavity and the carbamate network may remove the dye, the obtained polymer has a double advantage. Besides, the presence of quaternary ammonium groups enables QAS-β-CDPU to possess good bactericidal properties. When the quaternization degree is 60%, sterilization rate of QAS-β-CDPU comes to 97.3%. On basis of this simple, green and economical synthetic route, QAS-β-CDPU has the potential to become an ideal multifunctional material in water purification.
Work Environment Characteristics as Determinants of Work to Family Conflict Among Working Females, Egypt
Authors: Zayed Hanaa , Kabbash Ibrahim , Elsalamy Rania , Abdo Sanaa 
Published: February 2021

Abstract
BackgroundPositive work practice environment is the corner stone for control and relieving of work to family conflict (WFC). Objectiveso determine the prevalence of work to family conflict and to study the relation between work to family conflicts and work environment characteristics among full-time female employees at Tanta University Medical Campus. MethodsA cross sectional study was carried among female employees working in the five Faculties that present at Tanta University Medical Campus. A total of 442 female workers were recruited. Data were collected using a self-administered questionnaire including personal characteristics, work related data and home responsibilities. Job characteristics scales, work to family and family to work spillovers scales were used to study their correlation. ResultsParticipants’ mean age was 39.87 ± 10.07 years. Significant positive correlation was detected between; skills discretion and both of positive work to family and family to work spillovers (r= 0.361, r= 0.288, P=0. 0.001 respectively), similar relations were reported regarding decision authority, coworkers support and supervisors support. The negative work to family and family to work spillovers significantly increased by increasing in work demands (r= 0. 0.315, r= 0.218, P=0. 0.001 respectively). Significant and negative correlations were found between each of skills discretion & supervisors support scales and the negative impact of family to work spillover (r= -0.130; P= 0.006, r= -0.167; P= 0.001, respectively). ConclusionPositive work practices environment is a significant determinant to prevent and control work to family conflict.
Hg Concentrations and Stable Isotopes Variations in Tropical Fish Species of a Gold-mining Impacted Watershed in French Guiana
Authors: Laffont Laure , Menges Johanna , Goix Sylvaine , Gentes Sophie , Maury-Brachet Régine , Sonke Jeroen E. , Legeay Alexia , Gonzalez Patrice , Rinaldo Raphaëlle , Maurice Laurence 
Published: February 2021

Abstract
The aim of the study was to determine if gold mining activities could impact the mercury (Hg) concentrations and isotopic signatures in freshwater fish consumed by riparian people in French Guiana. Total Hg, MeHg concentrations and Hg stable isotopes ratios were analyzed in fish muscles from different species belonging to three feeding patterns (herbivorous, periphytophagous and piscivorous). We compared tributaries impacted by gold-mining activities (Camopi, CR) with a pristine area upstream (Trois-Sauts, TS), along the Oyapock River. We measured δ15N and δ13C to examine whether Hg patterns are due to differences in trophic level. Differences in δ15N and δ13C values between both studied sites were only observed for periphytophageous fish, with enriched values at TS. Total Hg concentrations and Hg stable isotopes fractionations showed that Hg accumulated in fish from both areas have undergone different biogeochemical processes. Δ199Hg variation in fish (-0.5 to 0.2‰) was higher than the ecosystem baseline defined by a Δ199Hg of -0.66‰ in sediments, and suggested limited aqueous photochemical MeHg degradation. Photochemistry-corrected δ202Hg in fish were 0.7‰ higher than the baseline, consistent with biophysical and chemical isotope fractionations in aquatic environment. While THg concentrations in periphytophagous fish were higher in the gold-mining area than in TS, in contrast to the sediments results, the ensemble of Hg isotope shifts did not allow to trace and quantify the gold-mining related liquid Hg(0) sources in the fish muscles.
Liver Abnormalities in Turner’s Syndrome – Effects of Estrogen Replacement Therapy
Authors: István Fedor , Zold Eva , Barta Zsolt 
Published: February 2021

Abstract
BackgroundTurner’s syndrome is one of the most frequently reported sex chromosomal abnormality, affecting approximately 40 in every 100,000 live female births. Due to insufficient estrogen production, induction of puberty and sexual development requires hormone replacement. The syndrome affects several organ systems with diverse clinical features (cardiovascular, reproductive, hepato-biliary). There is also an increased risk of developing immune-mediated inflammatory diseases (IMID). Hepatobiliary alterations embrace a broad spectrum of possible manifestations, from asymptomatic mild hypertransaminasemia to overt hepatitis and even cirrhosis. Although exogenous estrogen hormones might cause liver dysfunction, in Turner’s syndrome hormone replacement can even alleviate the derangement of laboratory values and might prove beneficial in preventing the progression of hepatic architectural alterations.FindingsWe report two patients, in whom cessation of estrogen replacement therapy lead to worsening of hepatic and cholestatic enzyme values. These changes were later alleviated by recommencing estrogen hormone administration. We aim to summarize the available literature on estrogen hormone replacement therapy in Turner’s syndrome. We also provide a brief overview on the role of estrogen hormones in the pathology associated with the syndrome. ConclusionsOur findings are confirming, that estrogen replacement therapy has beneficial effects on hepatic enzymes and liver related laboratory studies in Turner’s syndrome. Therefore it is recommended for physicians not to withdraw estrogen replacement, even with elevated concentrations of liver and cholestatic enzymes in Turner’s syndrome patients.
The Pattern of Knockdown Resistance Mutations Correlated to the Annual Average Temperature in Field Populations of Aedes Albopictus (Diptera: Culicidae) in China
Authors: Chen Hanming , Zhou Qiuming , Dong Haowei , Yuan Hao , Bai Jie , Gao Jingpeng , Tao Feng , Ma Hui , Li Xiangyu , Peng Heng , Ma Yajun 
Published: February 2021

Abstract
Background: Aedes albopictus is the main vector of dengue fever in China, distributed from north to south in China. Insecticides are an important method to control the mosquitoes, especially in the outbreak of dengue fever, but insecticide resistance raises the risk of failure to control vector-borne diseases. Knockdown resistance (kdr) caused by point mutations in the VGSC gene is a key mechanism that confers resistance to pyrethroids. To explore the characteristics and possible evolution trend of kdr mutation in Ae. albopictus, we analyzed the kdr mutations of field populations in China in this study.Methods: A total of 1 549 Ae. albopictus were collected from 18 sites in China from 2017 to 2019, as well as 50 individuals from three sites in the 1990s. A fragment of approximately 350 bp from part of S6 segment in the VGSC gene domain III was amplified and sequenced. The haplotypes of VGSC gene were recorded and the parsimony network was constructed using TCS 1.21. The data of annual average temperatures (AAT) of collection sites was acquired from national database. The correlation between AAT of the collection site and the kdr mutation rate was analyzed by Pearson Correlation using SPSS 21.0. Results: The overall frequency of mutant allele F1534 is 45.62%. Nine mutant alleles were detected at codon 1534 in fifteen field populations, namely TCC/TCG (S) (38.86%), TTG/CTG/CTC/TTA (L) (3.71%), TGC (C) (2.68%), CGC (R) (0.27%) and TGG (W) (0.10%). Only one mutant allele ACC (T) was found at codon 1532 with frequency of 6.39% in ten field populations. Moreover, multiple mutations at I1532 and F1534 in a sample appeared in five populations. The 1534 mutation rate was significantly positive related to AAT (Coefficient=0.624, p=0.0056), while the 1532 mutation rate was significantly negative related to AAT (Coefficient=-0.645, p=0.0038). Thirteen haplotypes were inferred, in which six mutant haplotypes were formed by one step, and the other six haplotypes were formed by one more mutations. In the samples from 1990s, no mutant allele was detected at codon 1532 of VGSC gene. However, F1534S/TCC was found in HNHK94 with an unexpected frequency of 100%.ConclusionsKdr mutations are widespread in the field populations of Ae. albopictus in China. Two novel mutant alleles F1534W/TGG and F1534R/CGC were the detected. The 1534 kdr mutation appeared in the population of Ae. albopictus no later than 1990s. F1534 mutation rate is positive correlated to AAT, while I1532 mutation rate is negative correlated to AAT. Insecticide using should be carefully managed to slow down the spread of high-resistance Ae. albopictus populations.
Biochemical Properties of an Alkaline Xylanase From Bacillus Agaradhaerens C9 and Its Application in Producing Xylo-oligosaccharides With High Degree of Polymerization From Wheat Bran
Authors: Liu Jiawen , Sun Di , Dong Zhen , Liu Cong , Zhu Jingrong , Liu Weijie 
Published: February 2021

Abstract
A xylanase of Bacillus agaradhaerens C9 was heterologously expressed and was then investigated. The recombinant xylanase (rBaxyl11) showed maximal activity at 60°C and pH 8.0-9.0. Under optimal conditions, Kcat of rBaxyl11 for arabinoxylan and glucuronoxylan were 599 s-1 and 330 s-1, respectively. rBaxyl11 showed a good stability at pH ranging from 5.0 to 9.0, and retained 50% of activity after 6-hour incubation at 70°C. However, it was markedly inactivated by transition elements including Fe3+, Ni2+, Mn2+, Co2+, Zn2+, Cu2+ and Fe2+. rBaxyl11 generated xylo-oligosaccharides (XOS) whose degree of polymerization (DP) is greater than 3 when hydrolyzing arabinoxylan, while the DP of XOS ranged from 2 to 6 when acting on glucuronoxylan. Simultaneously producing xylanase and XOS by recombinant E. coli containing rBaxyl11 were then carried out. Results showed that the engineering E. coli generated xylanase and high-DP XOS extracellularly using wheat bran as substrate, and concentration of XOS reached 73 mg/g substrate after 12-hour fermentation. This study indicates the feasibility of producing XOS by a single-step fermentation approach with low cost using rBaxyl11.
Fiber Connectivity Density Mapping in End-stage Renal Disease Patients: A Preliminary Study
Authors: Ma Chi , Jiang Xinghai , Ren Yande , Gu Gaojie , Fu Airong , Wang Chengjian , Bai Peirui , Zhou Tong , Qin Shanshan , Fu Shengli 
Published: February 2021

Abstract
Abnormal brain structural connectivity of end-stage renal disease (ESRD) is associated with cognitive impairment. However, the characteristics of cortical structural connectivity have not been investigated in ESRD patients. The current study aimed to investigate the structural connectivity of the entire cerebral cortex in ESRD patients. Twenty-five ESRD patients and 20 healthy controls were recruited for this study; all participants underwent diffusion tensor imaging and high-resolution T1-weighted imaging scanning. Fiber connectivity density (FiCD) mapping was performed to calculate structural connectivity of the whole cortex, and between-group differences were compared in a vertexwise manner. We also tested the associations between FiCD changes and clinical information using Pearson correlation analysis. The results demonstrated that the mean global FiCD value was significantly decreased in ESRD patients compared with that of HCs. Notably, FiCD values were significantly changed (decreased or increased) in certain cortical regions, which mainly involved the bilateral dorsolateral prefrontal cortex (DLPFC), inferior parietal, lateral temporal and middle occipital cortex (P < 0.01 with Monte Carlo correction). Moreover, in ESRD patients, the increased FiCD values in the right middle frontal gyrus were negatively correlated with serum creatinine (r=-0.473, p = 0.017) and urea levels (r=-0.511, p = 0.009). The increased FiCD values in the left middle frontal gyrus were negatively correlated with dialysis duration (r=-0.577, p = 0.003) and parathyroid hormone (PTH) levels (r=-0.552, p = 0.004). Our results suggested that ESRD patients exhibited extensive impaired cortical structural connectivity. And there may be a compensation mechanism of cortical structural recombination that plays a role in the way the brain adapts to maintain optimal network function. Moreover, serum creatinine, urea and PTH levels may be risk factors for brain structural network decompensation in ESRD patients.
A systematic review of school health policy measurement tools: Implementation determinants and outcomes
Authors: McLoughlin Gabriella M , Allen Peg , Walsh-Bailey Callie , Brownson Ross C. 
Published: February 2021

Abstract
Background

Governments in some countries or states/provinces mandate school-based policies intended to improve the health and well-being of primary and secondary students, and in some cases the health of school staff. Examples include mandating a minimum time spent per week in programmed physical activity, mandating provision of healthy foods and limiting fat content of school meals, and banning tobacco products or use on school campuses. Although school health researchers have studied whether schools, districts, or states/provinces are meeting requirements, it is unclear to what extent implementation processes and determinants are assessed. The purposes of the present systematic review of quantitative measures of school policy implementation are to identify quantitative measures of implementation determinants and Proctor model implementation outcomes and assess pragmatic and psychometric properties of such measures.
Methods

Peer-reviewed journal articles published 1995–2020 were included if they: 1) had multiple-item quantitative measures of school policy implementation; and 2) addressed overall wellness, tobacco, physical activity, nutrition, obesity prevention, and mental health/bullying/social-emotional learning. The final sample comprised 86 measurement tools from 67 peer-review articles. We extracted study characteristics, such as psychometric and pragmatic measure properties, from included articles based on three frameworks: Implementation Outcomes Framework, 2) Consolidated Framework for Implementation Research, and 3) Policy Implementation Determinants Framework.
Results

Most implementation tools were developed to measure overall wellness policies which combined multiple policy topics (n = 35, 40%) and were in survey form (n = 75, 87%). Fidelity was the most frequently prevalent implementation outcome (n = 70, 81%), followed by adoption (n = 32, 81%). The implementation determinants most assessed were readiness for implementation, including resources (n = 43, 50%), leadership (n = 42, 49%), and policy communication (n = 41, 48%). Overall, measures were low-cost and had easy readability. However, lengthy tools and lack of reported validity/reliability data indicate low transferability.
Conclusions

Implementation science can contribute to more complete and rigorous assessment of school health policy implementation processes, which can improve implementation strategies and ultimately the intended health benefits. Several high-quality measures of implementation determinants and implementation outcomes can be applied to school health policy implementation assessment. D&I researchers can also benefit from measurement experiences of school health researchers.