bluuebunny/crossref_metadata_2025_split huggingface dataset:
The movement-to-music (M2M) study: study protocol for a randomized controlled efficacy trial examining a rhythmic teleexercise intervention for people with physical disabilities
Authors: Young Hui-Ju , Lai Byron , Mehta Tapan , Thirumalai Mohanraj , Wilroy Jereme , Yates Alex , Kane Brandon , Rimmer James H. 
Published: November 2021

Abstract
Background
People with physical disabilities need exercise routines that are enjoyable, readily available in the home, adapted to their functional level, and eliminate common barriers to exercise participation related to transportation and time commitment. The purpose of the movement-to-music (M2M) study is to address these issues by establishing a remotely delivered, rhythmic exercise program for people with physical disabilities.

Methods
The study is a two-arm randomized controlled efficacy trial examining a 12-week remotely delivered M2M intervention (eM2M) in 108 people with physical disabilities. The primary outcomes are changes in cardiorespiratory fitness and muscle strength at post 12-week intervention.

Discussion
The eM2M study will enhance our understanding of an alternative intervention design and delivery mode that removes common barriers to exercise participation experienced by people with physical disabilities. The eM2M intervention may be an alternative option for people with physical disabilities to obtain regular exercise, especially during a pandemic when exercising in indoor facilities may be problematic.

Trial registration
ClinicalTrials.gov NCT03797378. Registered on January 9, 2019, with the trial name “Movement-to-Music: Lakeshore Examination of Activity, Disability, and Exercise Response Study (M2M LEADERS)”.
Genome-wide association study and transcriptome analysis dissect the genetic control of silique length in Brassica napus L.
Authors: Wang Jia , Fan Yueling , Mao Lin , Qu Cunmin , Lu Kun , Li Jiana , Liu Liezhao 
Published: November 2021

Abstract
Background
Rapeseed is the third-largest oilseed crop after soybeans and palm that produces vegetable oil for human consumption and biofuel for industrial production. Silique length (SL) is an important trait that is strongly related to seed yield in rapeseed. Although many studies related to SL have been reported in rapeseed, only a few candidate genes have been found and cloned, and the genetic mechanisms regulating SL in rapeseed remain unclear. Here, we dissected the genetic basis of SL by genome-wide association studies (GWAS) combined with transcriptome analysis.

Results
We identified quantitative trait locus (QTL) for SL using a recombinant inbred line (RIL) population and two independent GWAS populations. Major QTLs on chromosomes A07, A09, and C08 were stably detected in all environments from all populations. Several candidate genes related to starch and sucrose metabolism, plant hormone signal transmission and phenylpropanoid biosynthesis were detected in the main QTL intervals, such as BnaA9.CP12-2, BnaA9.NST2, BnaA7.MYB63, and BnaA7.ARF17. In addition, the results of RNA-seq and weighted gene co-expression network analysis (WGCNA) showed that starch and sucrose metabolism, photosynthesis, and secondary cell wall biosynthesis play an important role in the development of siliques.

Conclusions
We propose that photosynthesis, sucrose and starch metabolism, plant hormones, and lignin content play important roles in the development of rapeseed siliques.
Reliability and validity of the Mexican teachers’ physical activity questionnaire (MTPAQ) in a subsample of female Mexican teachers
Authors: Medina C. , Monge A. , Romero M. , López-Ridaura R. , Barquera S. , Romieu I. , Denova-Gutiérrez E. , Lajous M. 
Published: November 2021

Abstract
Background
Reliable and valid instruments are needed to estimate physical activity levels. The purposes of this study were to estimate the reliability and validity of the Physical Activity Questionnaire (MTPAQ) in a subsample of the Mexican Teachers Cohort study.

Methods
We completed telephone interviews and clinical examinations of 82 teachers. Two MTPAQ, five International Physical Activity Questionnaire (IPAQ)-long form, and two accelerometer (AC) measures were used to determine physical activity levels throughout 24 months. Moderate and walking physical activity (MWPA min/week), vigorous physical activity (VPA min/week), and moderate-to-vigorous physical activity minutes per week (MVPA min/week) were estimated for each instrument. Pearson, Intra-class correlations and deattenuated adjustments were used to determine the reliability and validity of MTPAQ.

Results
MWPA and MVPA min/week of MTPAQs were moderately correlated (r ≥ 0.54) to min/week of IPAQ-long form. MWPA and MVPA min/week average MTPAQ and MTPAQ1 and average AC, AC1 and AC2 were fairly correlated (r ≥ 0.20). A higher correlation was observed when correlation coefficients were attenuated (r ≥ 0.32).

Conclusions
MTPAQ1 is a reliable and valid tool to measure physical activity levels.
Association of FTO rs1421085 single nucleotide polymorphism with fat and fatty acid intake in Indonesian adults
Authors: Al-Jawadi Athraa Alaulddin , Priliani Lidwina , Oktavianthi Sukma , Febinia Clarissa A. , Daya Mulianah , Artika I Made , Malik Safarina G. 
Published: November 2021

Abstract
Objective
Recent studies showed that genetic polymorphisms in the fat mass and obesity-associated gene (FTO) were associated with obesity and dietary intake. In this study of 71 adults in Jakarta, Indonesia, we investigated FTO rs1421085 association with body mass index (BMI), macronutrient intake, and fatty acid intake. The association was evaluated using linear regression analyses assuming co-dominant, dominant, recessive, over-dominant, and additive genetic models.

Results
Only individuals with the CC genotype had a considerably higher BMI (p < 0.001), which indicates a recessive genetic trait, but the incidence for this genotype is low (68 TT + TC vs. 3 CC). Individuals with the minor C allele had an estimated increase of fat intake by 3.45–4.06% across various genetic models (dominant: p < 0.010, over-dominant: p < 0.030, additive: p < 0.010). Subjects with TC/CC genotypes had increased dietary monounsaturated fatty acid (MUFA; 1.14%, p = 0.046) and saturated fatty acid (SAFA; 2.06%, p = 0.023) intakes, compared to those with the TT genotype. In conclusion, our study provided evidence for the association between FTO rs1421085 risk allele with higher BMI and individual preferences for consuming more fat, MUFA, and SAFA. This study highlights the important role of FTO gene in food preference, and its influence on body weight.
Anti-metastatic effect of GV1001 on prostate cancer cells; roles of GnRHR-mediated Gαs-cAMP pathway and AR-YAP1 axis
Authors: Kim Ji Won , Park Miso , Kim Suntae , Lim Sung Chul , Kim Hyung Shik , Kang Keon Wook 
Published: November 2021

Abstract
Background
Gonadotropin-releasing hormone receptor (GnRHR) transmits its signal via two major Gα-proteins, primarily Gαq and Gαi. However, the precise mechanism underlying the functions of Gαs signal in prostate cancer cells is still unclear. We have previously identified that GV1001, a fragment of the human telomerase reverse transcriptase, functions as a biased GnRHR ligand to selectively stimulate the Gαs/cAMP pathway. Here, we tried to reveal the potential mechanisms of which GV1001-stimulated Gαs-cAMP signaling pathway reduces the migration and metastasis of prostate cancer (PCa) cells.

Methods
The expression of epithelial-mesenchymal transition (EMT)-related genes was measured by western-blotting and spheroid formation on ultra-low attachment plate was detected after GV1001 treatment. In vivo Spleen-liver metastasis mouse model was used to explore the inhibitory effect of GV1001 on metastatic ability of PCa and the transwell migration assay was performed to identify whether GV1001 had a suppressive effect on cell migration in vitro. In order to demonstrate the interaction between androgen receptor (AR) and YAP1, co-immunoprecipitation (co-IP), immunofluorescence (IF) staining, chromatin immunoprecipitation (ChIP) were performed in LNCaP cells with and without GV1001 treatment.

Results
GV1001 inhibited expression of EMT-related genes and spheroid formation. GV1001 also suppressed in vivo spleen-liver metastasis of LNCaP cells as well as cell migration in vitro. GV1001 enhanced the phosphorylation of AR and transcription activity of androgen response element reporter gene through cAMP/protein kinase A pathway. Moreover, GV1001 increased Ser-127 phosphorylation of YAP1 and its ubiquitination, and subsequently decreased the levels of AR-YAP1 binding in the promoter region of the CTGF gene. In contrast, both protein and mRNA levels of NKX3.1 known for tumor suppressor gene and AR-coregulator were upregulated by GV1001 in LNCaP cells. YAP1 knockout using CRISPR/Cas9 significantly suppressed the migration ability of LNCaP cells, and GV1001 did not affect the cell migration of YAP1-deficient LNCaP cells. On the contrary, cell migration was more potentiated in LNCaP cells overexpressing YAP5SA, a constitutively active form of YAP1, which was not changed by GV1001 treatment.

Conclusions
Overall, this study reveals an essential role of AR-YAP1 in the regulation of PCa cell migration, and provides evidence that GV1001 could be a novel GnRHR ligand to inhibit metastasis of PCa via the Gαs/cAMP pathway.
Beyond the role of participant: a firsthand account of the experiences of a patient-oriented research team
Authors: Boechler Lindsey , Renwick Steven , Alabi Abdullateef , de la Torre Harold , Kumar Susheel , Singh Harmanpreet , Xavier Roshan , Hector Dalise , McTaggart Lauren , Shrubsole Jennifer 
Published: November 2021

Abstract
Background
Immigrants often find accessing and navigating the healthcare system difficult upon arriving in Canada. Existing challenges of accessing healthcare due to differing cultural norms, language barriers, limited health literacy, and system complexity have been outlined extensively in literature; however, evidence-informed practices to mitigate these disparities have yet to be determined.
Our research team took a patient-oriented research (POR) approach to learn more about the lived experiences of immigrants as they attempt to access and navigate the health system upon immigrating to Canada. POR is a method that involves patients beyond the role of participant, recognizing the lived experiences of patients as expertise and empowering patient partners to drive research priorities. This approach empowers patient partners to steer the direction of research, ensuring the study is relevant and patient priorities are addressed.

Main body
In this article, we define POR and share our team’s experience of engaging in POR by providing a synopsis of team member recruitment, research priority establishment, and relationship building. We also share how joining forces with patient partners, rather than solely engaging with them as participants, benefits research endeavors and ensures patient priorities are addressed. Lastly, we present examples of how conducting POR leads to increased research capacity and personal growth for both patient partners and researchers.

Conclusion
Building the foundation of this study through the perspectives of patient partners has provided insight into the difficulties immigrants experience when attempting to access and navigate the health care system that can only be understood through first-hand experience. Engaging patients as active partners on research teams enhances the potential of strengthened patient engagement, increased patient commitment to treatment, and leads to improved health outcomes. Furthermore, POR provides researchers, patients, and those serving the community at hand, an opportunity to learn from one another.
Neuropathic pain in knee osteoarthritis
Authors: Güngör Demir Uğur , Demir Ali Nail , Toraman Naciye Füsun 
Published: November 2021

Abstract
Background
This study aimed to investigate the relationship between the neuropathic pain in knee osteoarthritis with the body composition, anthropometric and postural features, physical function, and quality of life.

Methods
Patients with primary knee osteoarthritis, 50–70 years of age, were included in the study and divided into Group 1 with neuropathic pain and Group 2 with no neuropathic pain according to Douleur Neuropathique-4. The groups were compared in terms of demographic, clinical, radiological, laboratory findings and anthropometric measurements, body composition, physical function tests, osteoarthritis severity, quality of life, and posturography.

Results
200 patients were included in the study. 98 (82.6% female) were in Group 1 and 102 (74.5% female) in Group 2. Age was higher in Group 1 compared to Group 2 [61 (50–70) and 57.5 (50–70), respectively, p = 0.03]. Symptom duration was also longer in Group 1 (5.21 ± 4.76 and 3.38 ± 3.58, p = 0.002). Body mass indices were 31.9 ± 5.6 and 30.1 ± 4.8 (p = 0.015). Kellgren–Lawrence class, Western Ontario and McMaster Osteoarthritis Index and Short Form-36 scores were more unfavorable in Group 1. Although fall risk was similar, stability and Fourier harmony indices were impaired in Group 1 compared to Group 2 especially when the visual and proprioceptive input was blocked.

Conclusions
Almost half of the patients with knee osteoarthritis had neuropathic pain which was associated with longer symptom duration and higher age, lower education, higher body mass index, more severe radilogical findings, worse pain perception, lower physical function and quality of life, and lower stability.
Teaching death: exploring the end of life in a novel undergraduate course
Authors: Kulp David , O’Neill Lynn , Quest Tammie , Tamasi Susan , Loudermilk Kim , Eisen Arri 
Published: November 2021

AbstractWestern culture discourages discussion of death and dying, especially with healthy emerging adults. Yet, research shows that engaging this population in conversations about death and dying is empowering and important for young people’s decision-making around and understanding of the end of life. We show that students are indeed ill-informed on such issues but that they desire to learn more. We describe and assess a pilot undergraduate course in palliative care addressing this need, and we demonstrate its success in engaging and educating students using pedagogical approaches built to develop a social and intellectual community of trust.
Decellularized versus cryopreserved pulmonary allografts for right ventricular outflow tract reconstruction during the Ross procedure: a meta-analysis of short- and long-term outcomes
Authors: Ahmed Adham , Ahmed Sarah , Varghese Kathryn S. , Mathew Dave M. , Pandey Roshan , Rogando Dillon O. , Salazar Stephanie A. , Fusco Peter J. , Levy Kenneth H. 
Published: November 2021

Abstract
Background
The ideal conduit for repair of the right ventricular outflow tract (RVOT) during the Ross procedure remains unclear and has yet to be fully elucidated. We perform a pairwise meta-analysis to compare the short-term and long-term outcomes of decellularized versus cryopreserved pulmonary allografts for RVOT reconstruction during the Ross procedure.

Main body
After a comprehensive literature search, studies comparing decellularized and cryopreserved allografts for patients undergoing RVOT reconstruction during the Ross procedure were pooled to perform a pairwise meta-analysis using the random-effects model. Primary outcomes were early mortality and follow-up allograft dysfunction. Secondary outcomes were reintervention rates and follow-up endocarditis. A total of 4 studies including 1687 patients undergoing RVOT reconstruction during the Ross procedure were included. A total of 812 patients received a decellularized pulmonary allograft, while 875 received a cryopreserved pulmonary allograft. Compared to cryopreserved allografts, the decellularized group showed similar rates of early mortality (odds ratio, 0.55, 95% confidence interval, 0.21–1.41, P = 0.22). At a mean follow-up period of 5.89 years, no significant difference was observed between the two groups for follow-up allograft dysfunction (hazard ratio, 0.65, 95% confidence interval, 0.20–2.14, P = 0.48). Similarly, no difference was seen in reintervention rates (hazard ratio, 0.54, 95% confidence interval, 0.09–3.12, P = 0.49) nor endocarditis (hazard ratio, 0.30, 95% confidence interval, 0.07–1.35, P = 0.12) at a mean follow-up of 4.85 and 5.75 years, respectively.

Conclusions
Decellularized and cryopreserved pulmonary allografts are associated with similar postoperative outcomes for RVOT reconstruction during the Ross procedure. Larger propensity-matched and randomized control trials are necessary to elucidate the efficacy of decellularized allografts compared to cryopreserved allografts in the setting of the Ross.
Value of susceptibility weighted imaging (SWI) in assessment of intra-arterial thrombus in patients with acute ischemic stroke
Authors: Abdelgawad Ehab Ali , Amin Mohammed F. , Abdellatif Ahmed , Mourad Mohamed Aboulfotoh , Abusamra Manal F. 
Published: November 2021

Abstract
Background
Ischemic stroke is a major cause of death and disability. Thrombolytic therapy is a standard treatment stroke nowadays for ischemic strokes up to 4.5 h from start of symptoms. Although arterial occlusion can be detected by digital subtraction angiography (DSA), magnetic resonance angiography (MRA), and computed tomography angiography (CTA), the question about thrombus composition and formation times still might not be replied. The use of susceptibility weighted imaging (SWI) for detecting thrombus in acute ischemic stroke is getting to be a strongly investigated field. SWI can show the thrombus as a hypointense susceptibility vessel sign (SVS) in the affected area.

Results
Ninety-seven of our patients showed thrombus in MRA study. M1 segment was the most affected MCA segment representing about 57.6%. SWI detected intra-arterial thrombus in 122 patients compared to 97 patients detected by MRA (P = 0.0002). All patients had positive susceptibility sign. 88.8% of patients with positive thrombus in SWI had solitary thrombus, and 11.2% has multiple thrombi; on the other hand, MRA fails to detect any distant thrombi. 81% of patients with abnormally prominent vessel sign (APVS) showed parenchymal changes in these areas. On the other hand, deep structures, namely caudate nucleus, internal capsule and lentiform nucleus, are the least affected areas. All patients with abnormally prominent vessel sign showed arterial occlusion, and only 9 patients with no APVS showed arterial occlusion (P = 0.0001).

Conclusion
SWI plays an important role in the detection of peripheral thrombi in patients with acute ischemic stroke. Both SWI and MRA might complement each other for visual detection of occluded vessel. We recommend implementation of SWI into routine acute stroke MRI protocols.
Scaling up sexually transmissible infections point-of-care testing in remote Aboriginal and Torres Strait Islander communities: healthcare workers’ perceptions of the barriers and facilitators
Authors: Lafferty Lise , Smith Kirsty , Causer Louise , Andrewartha Kelly , Whiley David , Badman Steven G. , Donovan Basil , Anderson Lorraine , Tangey Annie , Mak Donna , Maher Lisa , Shephard Mark , Guy Rebecca ,   on behalf of the TTANGO2 Collaboration, Bastian Lisa , Ward James , Kaldor John , Comerford Crissy , Bushby Trish , Moore Liz , Gunathilake Manoji , Johnson David , Gallant Daniel 
Published: November 2021

Abstract
Background
Sexually transmissible infections (STIs), such as gonorrhoea and chlamydia, are highly prevalent, particularly in remote Aboriginal and Torres Strait Islander communities in Australia. In these settings, due to distance to centralised laboratories, the return of laboratory test results can take a week or longer, and many young people do not receive treatment, or it is considerably delayed. Point-of-care testing (POCT) provides an opportunity for same day diagnosis and treatment. Molecular POC testing for STIs was available at 31 regional or remote primary health care clinic sites through the Test-Treat-And-GO (TANGO2) program. This qualitative study sought to identify barriers and facilitators to further scaling up STI POCT in remote Aboriginal communities within Australia.

Methods
A total of 15 healthcare workers (including nurses and Aboriginal health practitioners) and five managers (including clinic coordinators and practice managers) were recruited from remote health services involved in the TTANGO2 program to participate in semi-structured in-depth interviews. Health services’ clinics were purposively selected to include those with high or low STI POCT uptake. Personnel participants were selected via a hybrid approach including nomination by clinic managers and purposive sampling to include those in roles relevant to STI testing and treatment and those who had received TTANGO2 training for POCT technology. Milat’s scaling up guide informed the coding framework and analysis.

Results
Acceptability of STI POCT technology among healthcare workers and managers was predominantly influenced by self-efficacy and perceived effectiveness of POCT technology as well as perceptions of additional workload burden associated with POCT. Barriers to integration of STI POCT included retention of trained staff to conduct POCT. Patient reach (including strategies for patient engagement) was broadly considered an enabler for STI testing scale up using POCT technology.

Conclusions
Remote healthcare clinics should be supported by both program and clinic management throughout scaling up efforts to ensure broad acceptability of STI POCT as well as addressing local health systems’ issues and identifying and enhancing opportunities for patient engagement.
Certification of a Tourist Destination As an Intellectual Potential for the Development of Tourism in the Region
Authors: Kuznecov A. , Shelonaev S. , Smetanina T. 
Published: November 2021

World and domestic experience shows that it is very effective to use the method of "tourist destinations" for the development of complex programs or strategies for tourism (1). As a result of numerous studies, a fairly coherent system of describing a "tourist destination" has been developed, allowing you to have a fairly comprehensive idea of both the product itself and the conditions for its receipt, attractiveness and related products. At the same time, the task of entrepreneurs, investors and representatives of regional authorities responsible for the tourism industry is to determine the prospects for the development of a particular tourist product or route. To do this, it is necessary to solve the problem of determining the investment attractiveness and prospects of a particular tourist destination, determine its competitiveness in relation to those available in this region and make a decision on the priority and expediency of investing in this direction of the tourism industry.
This article is an attempt of the authors to formalize the process of determining the priority and promising development of regional tourism directions in the region, using the example of the tourism industry of Udmurtia. The basis is the preparation of a passport of a tourist destination and the use of expert assessments.