bluuebunny/crossref_metadata_2025_split huggingface dataset:
Molecular analysis of pediatric CNS-PNET revealed nosologic heterogeneity and potent diagnostic markers for CNS neuroblastoma with FOXR2-activation
Authors: Korshunov Andrey , Okonechnikov Konstantin , Schmitt-Hoffner Felix , Ryzhova Marina , Sahm Felix , Stichel Damian , Schrimpf Daniel , Reuss David E. , Sievers Philipp , Suwala Abigail Kora , Kumirova Ella , Zheludkova Olga , Golanov Andrey , Jones David T. W. , Pfister Stefan M. , Kool Marcel , von Deimling Andreas 
Published: February 2021

AbstractPrimitive neuroectodermal tumors of the central nervous system (CNS-PNETs) are highly malignant neoplasms posing diagnostic challenge due to a lack of defining molecular markers. CNS neuroblastoma with forkhead box R2 (FOXR2) activation (CNS_NBL) emerged as a distinct pediatric brain tumor entity from a pool previously diagnosed as primitive neuroectodermal tumors of the central nervous system (CNS-PNETs). Current standard of identifying CNS_NBL relies on molecular analysis. We set out to establish immunohistochemical markers allowing safely distinguishing CNS_NBL from morphological mimics. To this aim we analyzed a series of 84 brain tumors institutionally diagnosed as CNS-PNET. As expected, epigenetic analysis revealed different methylation groups corresponding to the (1) CNS-NBL (24%), (2) glioblastoma IDH wild-type subclass H3.3 G34 (26%), (3) glioblastoma IDH wild-type subclass MYCN (21%) and (4) ependymoma with RELA_C11orf95 fusion (29%) entities. Transcriptome analysis of this series revealed a set of differentially expressed genes distinguishing CNS_NBL from its mimics. Based on RNA-sequencing data we established SOX10 and ANKRD55 expression as genes discriminating CNS_NBL from other tumors exhibiting CNS-PNET. Immunohistochemical detection of combined expression of SOX10 and ANKRD55 clearly identifies CNS_NBL discriminating them to other hemispheric CNS neoplasms harboring “PNET-like” microscopic appearance. Owing the rarity of CNS_NBL, a confirmation of the elaborated diagnostic IHC algorithm will be necessary in prospective patient series.
Rapid water parcel transport across the Kuroshio Extension in the lower thermocline from dissolved oxygen measurements by Seaglider
Authors: Hosoda Shigeki , Inoue Ryuichiro , Nonaka Masami , Sasaki Hideharu , Sasai Yoshikazu , Hirano Mizue 
Published: February 2021

AbstractSmall water parcels, which are characterized by a low salinity and high dissolved oxygen (DO) are observed by Seaglider in the main thermocline (26.0–27.0 σθ) south of the Kuroshio Extension (KE), have horizontal and vertical scales of a few tens of kilometers and a few tens of meters, respectively. Water mass analyses revealed larger negative salinity anomalies (<− 0.05) and positive DO anomalies (> 15 μmol kg−1) than those of the surrounding water. The characteristics are similar to those of water mass with low salinity and high DO in the subpolar Northwestern Pacific Ocean. Additionally, higher DO anomaly water parcels appear in the upper layer (< 26.7 σθ) while low salinity parcels appear in the lower layer (> 26.7 σθ). Oxygen consumption rates from the apparent oxygen utility suggest that the small water parcels consume less oxygen than the surrounding water, implying that they migrate in a shorter time across the KE after subduction and their characteristics may reflect the sea surface temperature, salinity, and DO in the subduction region. Similar small water parcels represented by high-resolution numerical simulations indicate that they pass through the KE in 1 month. The simulations support the oxygen consumption rate from the Seaglider observations. The existence of a fast process for water mass migration via meso- and submesoscale subduction processes across the KE affects the amount, subduction, and exchange process of water mass. Our study indicates that a small water mass contributes to the exchange process across the KE rapidly, which had not been identified in previous studies. Consequently, detailed observations using multiple Seagliders should capture detailed spatial and temporal variability of the water mass exchange process.
Jejunal limb obstruction by a tumor thrombus from pancreatic metastasis of renal cell carcinoma: a case report
Authors: Sakamoto Shinya , Matsumura Masaru , Tani Keigo , Nemoto Satoshi , Tsuchida Kazuhito , Koga Fumitaka , Seyama Yasuji 
Published: February 2021

Abstract
Background
Renal cell carcinoma (RCC) is a primary tumor with the highest frequency of pancreatic metastasis. Although surgical resection can improve the prognosis of some patients with pancreatic metastasis of RCC (PM-RCC), the role of palliative surgery remains unclear. Herein, we described a case of jejunal limb occlusion caused by a tumor thrombus arising from a PM-RCC which was treated by surgical resection.

Case presentation
A 75-year-old, male patient with metastatic RCC was admitted to our hospital with new-onset dysphagia and weight loss. Twenty years earlier he underwent a right nephrectomy with an adrenalectomy for the first surgical resection of RCC, and 12 years ago he underwent a left partial nephrectomy for metachronous primary RCC. Nine years later, multiple pancreatic metastases were detected. After discontinuing interferon therapy, he was followed up at his request without anticancer treatment. Multiple, pulmonary metastases developed 3 years ago, and resection of a brain metastasis was performed 6 months ago. He had also undergone a total gastrectomy with Roux-en Y reconstruction and splenectomy for gastric cancer 23 years ago. Computed tomography revealed a metastatic lesion in the pancreatic tail extending into the jejunal limb, which was obstructed by a tumor thrombus. Jejunal limb resection was performed concomitantly with a distal pancreatectomy as palliative surgery. The jejunal limb remnant was approximately 30 cm long and was re-anastomosed to the esophagus using a circular stapler. Blood perfusion at the anastomotic site was confirmed by indocyanine green fluorescence imaging. He was discharged on postoperative day 24 and was followed in the outpatient clinic. He achieved sufficient oral intake at 8 months postoperatively.

Conclusions
PM-RCC can invade the gastrointestinal tract and cause tumor thrombus formation resulting in bowel occlusion requiring surgical intervention.
Intermittent fasting: is there a role in the treatment of diabetes? A review of the literature and guide for primary care physicians
Authors: Albosta Michael , Bakke Jesse 
Published: February 2021

AbstractBackgroundType 2 Diabetes is a metabolic disorder characterized by hyperglycemia that causes numerous complications with significant long-term morbidity and mortality. The disorder is primarily due to insulin resistance particularly in liver, skeletal muscle, and adipose tissue. In this review, we detail the hormonal mechanisms leading to the development of diabetes and discuss whether intermittent fasting should be considered as an alternative, non-medicinal treatment option for patients with this disorder.MethodsWe searched PubMed, Ovid MEDLINE, and Google Scholar databases for review articles, clinical trials, and case series related to type 2 diabetes, insulin resistance, and intermittent fasting. Articles were carefully reviewed and included based on relevance to our topic. We excluded abstracts and any non-English articles.ResultsThe majority of the available research demonstrates that intermittent fasting is effective at reducing body weight, decreasing fasting glucose, decreasing fasting insulin, reducing insulin resistance, decreasing levels of leptin, and increasing levels of adiponectin. Some studies found that patients were able to reverse their need for insulin therapy during therapeutic intermittent fasting protocols with supervision by their physician.ConclusionCurrent evidence suggests that intermittent fasting is an effective non-medicinal treatment option for type 2 diabetes. More research is needed to delineate the effects of intermittent fasting from weight loss. Physicians should consider educating themselves regarding the benefits of intermittent fasting. Diabetic patients should consult their physician prior to beginning an intermittent fasting regimen in order to allow for appropriate oversight and titration of the patients medication regimen during periods of fasting.
Plasma proteome profiling of cardiotoxicity in patients with diffuse large B-cell lymphoma
Authors: Mörth Charlott , Sabaa Amal Abu , Freyhult Eva , Christersson Christina , Hashemi Jamileh , Hashemi Nashmil , Kamali-Moghaddam Masood , Molin Daniel , Höglund Martin , Eriksson Anna , Enblad Gunilla 
Published: February 2021

Abstract
Background
Cardiovascular toxicity is a notorious complication of doxorubicin (DXR) therapy for diffuse large B-cell lymphoma (DLBCL). Although surveillance of well-known biological markers for cardiovascular disease (CVD) as NTproBNP and Troponins may be helpful, there are no established markers to monitor for evolving CVD during treatment. New possibilities have arisen with the emergence of newer techniques allowing for analysis of plasma proteins that can be associated with cardiovascular disease. Proximity Extension Assay is one of them.

Objectives
We aimed to illustrate the incidence of CVD in DLBCL patients treated with DXR and to establish whether there are plasma proteins associated with pre-existing or emerging CVD.

Methods
In 95 patients, 182 different proteins from OLINK panels, NTproBNP, Troponin I and CRP were assessed prior to, during and after treatment. For comparison, samples from controls were analyzed.

Results
In the DLBCL cohort, 33.3% had pre-treatment CVD compared to 5.0% in the controls and 23.2% developed new CVD. Of the 32.6% who died during follow up, CVD was the cause in 4 patients. Spondin-1 (SPON-1) correlated to pre-treatment CVD (1.22 fold change, 95% CI 1.10–1.35, p = 0.00025, q = 0.045). Interleukin-1 receptor type 1 (IL-1RT1) was associated to emerging CVD (1.24 fold change, 95% CI 1.10–1.39, p = 0.00044, q = 0.082).

Conclusion
We observed a higher prevalence of CVD in DLBCL patients compared to controls prior to DXR therapy. Two proteins, SPON-1 and IL-1RT1, were related to pre-existing and emerging CVD in DXR treated patients. If confirmed in larger cohorts, IL-1RT1 may emerge as a reliable biomarker for unfolding CVD in DLBCL.
Creation of an ex-vivo bovine kidney flow model for testing embolic agents: work in progress
Authors: Garza Luis , Bitar Ryan , O’Donnell Barrett , Parker Matthew , Ortiz Carlos , Hyman Charles , Walker John , Song Ho-Young , Lopera Jorge 
Published: February 2021

AbstractObjectivesTo develop an ex- vivo perfusion flow model using a bovine kidney for future testing of embolic agents in an inexpensive and easy way.MethodsSix bovine adult kidneys were used for this study. Kidneys were cannulated and perfused via a roller pump. Three embolic agents, coils, Gelfoam, and a glue mixture of Histoacryl + Lipiodol, were deployed by targeting three secondary segmental arteries per kidney via a 5Fr catheter under fluoroscopic guidance. Cannulation time, success rate of segmental artery selection and embolic agent deployment, total operational time, and fluoroscopy dose were recorded.ResultsAverage kidney weight was 0.752 +/− 0.094 kg. All six bovine kidneys were successfully cannulated in 21.6 min +/− 3.0 min. Deployment of coils and glue was achieved in every case (12/12); however, Gelfoam injection was not successful in one instance (5/6, 83%). Coil deployment demonstrated no embolic effect while Gelfoam and glue injections demonstrated decreased distal contrast filling post-embolization. Mean dose area product was 12.9 ± 1.8 Gy·cm2, fluoroscopy time was 10 ± 4 min and operational time was 27 ± 8 min.ConclusionsWe describe the creation of an ex vivo bovine kidney flow model for the preclinical evaluation of different embolic materials. The flow model can be modified to provide extensive bench testing and it is a promising tool for hands -on training in basic and advanced embolization techniques .
ABO groups can play a role in susceptibility and severity of COVID-19
Authors: Samra S. , Habeb M. , Nafae R. 
Published: February 2021

Abstract
Background
A few people infected by the coronavirus become seriously ill, while others show little to no signs of the symptoms, or are asymptomatic. Recent researches are pointing to the fact that the ABO blood group might play an important role in a person’s susceptibility and severity of COVID-19 infection. Aim of the study: try to understand the relationship between ABO groups and COVID-19 (susceptibility and severity).

Results
A total of (507) patients were included in this study. The study population was divided based on the ABO blood group into types A+, A−, B+, AB, O+, and O−. Blood group A was associated with high susceptibility of infection: group A, 381 (75.1%); and less common in group O, 97 (19.2%), group B, 18 (3.5%), and group AB, 11 (2.2%). The severity of COVID-19 infection was common in non-blood group O where (20 (7.1%), 4 (26.7%), 2 (11%), and 1 (9%) in type A+, A−, B+, and AB, respectively), while in type O 3.1%. And mechanically ventilated patients were 22 (5.9%), 2 (13.4%), 2 (11.1%), and 1 (1%). Mortality was high in blood groups A and B, 16 (4.37%) and 1 (5.5%), respectively, while in blood group O, it was 1%.

Conclusion
The incidence, severity, and mortality of COVID-19 were common in non-blood group O. While blood group O was protected against COVID-19.
Application of time series prediction techniques for coastal bridge engineering
Authors: Yu Enbo , Wei Huan , Han Yan , Hu Peng , Xu Guoji 
Published: February 2021

AbstractIn this study, three machine learning techniques, the XGBoost (Extreme Gradient Boosting), LSTM (Long Short-Term Memory Networks), and ARIMA (Autoregressive Integrated Moving Average Model), are utilized to deal with the time series prediction tasks for coastal bridge engineering. The performance of these techniques is comparatively demonstrated in three typical cases, the wave-load-on-deck under regular waves, structural displacement under combined wind and wave loads, and wave height variation along with typhoon/hurricane approaching. To enhance the prediction accuracy, a typical data preprocessing method is adopted and an improved prediction framework for the LSTM model after the rolling forecast prediction is proposed. The obtained results show that: (a) When making a prediction on data featured with periodic regularity, both the XGBoost and ARIMA models perform well, and the XGBoost model can make predictions multi-step ahead, (b) The ARIMA model can predict just one step ahead based on aperiodic dataset with limited amplitude more accurately, while the XGBoost and LSTM models can predict multi-step ahead with appropriate data preprocessing, and (c) All the three models can predict the data tendency with model updating over time, but the prediction accuracy of the LSTM model is more favorable. The successful application of these three machine learning techniques can provide guidance to resolve engineering problems with time-history prediction requirements.
How Patients and Providers Weigh the Risks and Benefits of Long-Term Opioid Therapy for Cancer Pain
Authors: Giannitrapani Karleen F. , Fereydooni Soraya , Silveira Maria J. , Azarfar Azin , Glassman Peter A. , Midboe Amanda , Zenoni Maria , Becker William C. , Lorenz Karl A. 
Published: February 2021

PURPOSE: To understand how patients and providers weigh the risks and benefits of long-term opioid therapy (LTOT) for cancer pain. METHODS: Researchers used VA approved audio-recording devices to record interviews. ATLAS t.i., a qualitative analysis software, was used for analysis of transcribed interview data. Participants included 20 Veteran patients and 20 interdisciplinary providers from primary care– and oncology-based practice settings. We conducted semistructured interviews and analyzed transcripts used thematic qualitative methods. Interviews explored factors that affect decision making about appropriateness of LTOT for cancer related pain. We saturated themes for providers and patients separately. RESULTS: Factors affecting patient decision-making included influence from various information sources, persuasion from trusted providers, and sometimes deferral of the decision to their provider. Relative prioritization of pain management as the focal patient concern varied with some patients describing comparatively more fear of chemotherapy than opioid analgesics, comparatively more knowledge of opioids in relation to other drugs;patients expressed a preference to spend the limited time they have with their oncologist discussing cancer treatment rather than opioid use. Factors affecting provider decision making included prognosis, patient goals, patient characteristics, and provider experience and biases. Providers differed in how they weigh the relative importance of alleviating pain or avoiding opioids in the face of treating patients with cancer and histories of substance abuse. CONCLUSION: Divergent perspectives on factors need to be considered when weighing risks and benefits. Policies and interventions should be designed to reduce variation in practice to promote equal access to adequate pain management. Improved shared decision-making initiatives will take advantage of patient decision-making factors and priorities.
18F-FDG-PET/CT Evaluation of Indeterminate Adrenal Masses in Noncancer Patients
Authors: He Xin , Caoili Elaine M , Avram Anca M , Miller Barbra S , Else Tobias 
Published: February 2021

Abstract

Context
Adrenal tumors in noncancer patients are common.

Objective
Evaluate performance of 18F-fluorodeoxyglucose positron emission tomography computed tomography (18F-FDG-PET/CT) in distinguishing between benign and malignant adrenal tumors.

Design
Retrospective chart review 2010-2019.

Setting
Academic institution.

Patients
One hundred and seventeen noncancer patients, defined as having no history of cancer or with cancer in remission for ≥5 years, completed 18F-FDG-PET/CT to evaluate adrenal masses, with pathologic diagnoses or imaging follow-up (≥12 months).

Intervention
18F-FDG-PET/CT of 117 indeterminate adrenal masses.

Main Outcome Measures
Receiver operator characteristic curve of the ratios of adrenal lesion standardized uptake value (SUV)max to liver SUVmean and of adrenal lesion SUVmax to aortic arch blood pool SUVmean were constructed.

Results
Seventy benign and 47 malignant masses (35 adrenocortical carcinomas [ACCs], 12 adrenal metastases) were identified. Malignant masses had higher median liver SUV and blood pool SUV ratios than benign masses (6.2 and 7.4 vs 1.4 and 2.0, P &lt; .001). Median liver and blood pool SUV ratios of ACC (6.1 and 7.3, respectively) and metastases (6.7 and 7.7, respectively) were higher than those of than adenomas (1.4 and 2.2, P &lt; .05 for all comparisons). Optimal liver SUV ratio to discern between benign and malignant masses was 2.5, yielding 85% sensitivity, 90% specificity, and 7 false negative results (including 3 ACCs). Optimal blood pool SUV ratio was 3.4, yielding 83% sensitivity, 90% specificity, and 8 false negative results (including 4 ACCs).

Conclusion
When used in conjunction with other clinical assessments, 18F-FDG-PET/CT can be a valuable tool in evaluating adrenal masses in noncancer patients.
Vacuolar protein Tag1 and Atg1–Atg13 regulate autophagy termination during persistent starvation in S. cerevisiae
Authors: Kira Shintaro , Noguchi Masafumi , Araki Yasuhiro , Oikawa Yu , Yoshimori Tamotsu , Miyahara Aiko , Noda Takeshi 
Published: February 2021

ABSTRACT
Under starvation conditions, cells degrade their own components via autophagy in order to provide sufficient nutrients to ensure their survival. However, even if starvation persists, the cell is not completely degraded through autophagy, implying the existence of some kind of termination mechanism. In the yeast Saccharomyces cerevisiae, autophagy is terminated after 10–12 h of nitrogen starvation. In this study, we found that termination is mediated by re-phosphorylation of Atg13 by the Atg1 protein kinase, which is also affected by PP2C phosphatases, and the eventual dispersion of the pre-autophagosomal structure, also known as the phagophore assembly site (PAS). In a genetic screen, we identified an uncharacterized vacuolar membrane protein, Tag1, as a factor responsible for the termination of autophagy. Re-phosphorylation of Atg13 and eventual PAS dispersal were defective in the Δtag1 mutant. The vacuolar luminal domain of Tag1 and autophagic progression are important for the behaviors of Tag1. Together, our findings reveal the mechanism and factors responsible for termination of autophagy in yeast.
Maternal provisioning and fluctuating thermal regimes enhance immune response in a reptile with temperature-dependent sex determination
Authors: Leivesley Jessica Alice , Rollinson Njal 
Published: February 2021

ABSTRACTThe Charnov–Bull model of differential fitness is often used to explain the evolution and maintenance of temperature-dependent sex determination (TSD). Most tests of the model focus on morphological proxies of fitness, such as size traits, whereas early life physiological traits that are closely related to lifetime fitness might provide a framework for generalizing the Charnov–Bull model across taxa. One such trait is the strength of the early-life immune response, which is strongly linked to early-life survival and fitness. Here, we manipulated temperature, variance in temperature, and sex to test the Charnov–Bull model using a physiological trait, immune system strength, in the snapping turtle (Chelydra serpentina). We found no evidence of sex-specific differences in bactericidal capacity of hatchling blood, and no evidence that mean temperature influences bactericidal capacity. However, we did find that fluctuating incubation temperature (i.e. a more naturalized incubation regime) is associated with a greater bactericidal capacity compared with constant temperature incubation. We also found that egg mass, a proxy for maternal provisioning, is positively associated with bactericidal capacity. Our findings suggest that the evolution of temperature-dependent sex determination in reptiles is unrelated to our measure of early-life innate immunity. Our study also underlines how immune response is condition dependent in early life, and questions the biological relevance of constant temperature incubation in experimental studies on ectotherm development.