bluuebunny/crossref_metadata_2025_split huggingface dataset:
N‐6 methylation‐related lncRNA is potential signature in lung adenocarcinoma and influences tumor microenvironment
Authors: Zheng Jian , Zhao Zhuochen , Wan Junhu , Guo Manman , Wang Yangxia , Yang Zhengwu , Li Zhuofang , Ming Liang , Qin Zhaobing 
Published: September 2021

AbstractBackgroundN‐6 methylation (m6A) pushes forward an immense influence on the occurrence and development of lung adenocarcinoma (LUAD). However, the methylation on non‐coding RNA in LUAD, especially long non‐coding RNA (lncRNA), has not been received sufficient attention.MethodsSpearman correlation analysis was used to screen lncRNA correlated with m6A regulators expression from the Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) repositories, respectively. Then, the least absolute shrinkage and selection operator (LASSO) was applied to build a risk signature consisting m6A‐related lncRNA. Univariate and multivariate independent prognostic analysis were applied to evaluate the performance of signature in predicting patients' survival. Next, we applied Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), and gene set enrichment analysis (GSEA) to conduct pathway enrichment analysis of 3344 different expression genes (DEGs). Finally, we set up a competing endogenous RNAs (ceRNA) network to this lncRNA.ResultsA total of 85 common lncRNAs were selected to acquire the components related to prognosis. The final risk signature established by LASSO regression contained 11 lncRNAs: ARHGEF26‐AS1, COLCA1, CRNDE, DLGAP1‐AS2, FENDRR, LINC00968, TMPO‐AS1, TRG‐AS1, MGC32805, RPARP‐AS1, and TBX5‐AS1. M6A‐related lncRNA risk score could predict the prognostic of LUAD and was significantly associated with clinical pathological. And in the evaluation of lung adenocarcinoma tumor microenvironment (TME) by using ESTIMATE algorithm, we found a statistically significant correlation between risk score and stromal/immune cells.ConclusionM6A‐related lncRNA was a potential prognostic and therapy target for lung adenocarcinoma.
Expression of fatty acid‐binding protein‐4 in gastrointestinal stromal tumors and its significance for prognosis
Authors: Zang Wei‐Jie , Wang Zi‐Niu , Hu Yi‐Lin , Huang Hua , Ma Peng 
Published: September 2021

AbstractBackgroundFatty acid‐binding proteins (FABPs) have been found to be involved in tumorigenesis and development. However, the role of FABP4, a member of the FABPs, in GISTs (Gastrointestinal stromal tumors) remains unclear. This study aimed to investigate the expression of FABP4 and its prognostic value in GISTs.MethodsFABP4 expression in 125 patients with GISTs was evaluated by immunohistochemical analysis of tissue microarrays. The relationship between FABP4 expression and clinicopathological features and prognosis of GISTs was analyzed.ResultsMultiple logistic regression analysis showed that expression of FABP4 correlated with tumor size and mitotic index. Furthermore, FABP4 level, tumor size, mitotic index, and high AFIP‐Miettinen risk were independent prognostic factors in GISTs. The Kaplan‐Meier survival curve showed that the 5‐year survival rate of patients with high‐FABP4 expression GISTs was lower.ConclusionsThese results suggested that high‐FABP4 expression might be a marker of malignant phenotype of GISTs and poor prognosis.
Utilizing the couple relationship to prevent suicide: A preliminary examination of Treatment for Relationships and Safety Together
Authors: Khalifian Chandra E. , Leifker Feea R. , Knopp Kayla , Wilks Chelsey R. , Depp Colin , Glynn Shirley , Bryan Craig , Morland Leslie A. 
Published: September 2021

AbstractObjectiveTo evaluate the feasibility, preliminary effects, and acceptability of the first comprehensive couple‐based treatment for suicide, called Treatment for Relationships and Safety Together (TR&ST).MethodIn a preliminary examination, five couples (N = 10) participated in 10 weekly sessions of TR&ST. All couples included a veteran who reported active suicidal ideation at baseline and their partner. Couples completed measures of relationship functioning, perceived burdensomeness, thwarted belonging, and suicidal ideation at baseline, mid‐treatment, and posttreatment.ResultsTR&ST was feasible to deliver. Veteran and partner relationship functioning improved and veteran perceived burdensomeness, thwarted belonging, and suicidal ideation decreased. There were no suicide related behaviors, hospitalizations, or crisis line calls during the study. TR&ST seemed acceptable to couples (100% retention and high satisfaction ratings).ConclusionCouple‐based suicide prevention may provide an additional avenue for suicide prevention in veterans.
“Halfway Independent”: Experiences of formerly homeless adults living in permanent supportive housing
Authors: Pilla David , Park‐Taylor Jennie 
Published: September 2021

AbstractPermanent supportive housing (PSH), which combines affordable public housing with social services, has become the dominant model in the United States for providing housing to formerly homeless people. PSH has been effective in reducing re‐entry to homelessness, yet has shown limited evidence of improving formerly homeless individuals' mental health and quality of life. This study aimed to understand the lived experiences of formerly homeless adults' adjustment to tenancy in PSH, with a focus on how living in PSH has affected their meaningful activity and social engagement. Based on a phenomenological approach, a thematic analysis was conducted using semi‐structured interviews with 17 individuals living in three PSH buildings in New York City. Results suggested that PSH was beneficial in fulfilling formerly homeless individual's basic needs and facilitating lifestyle improvements, yet many were dissatisfied with their living conditions and lacked meaningful activity, social integration, and community belongingness. These issues were found to develop in large part as a result of formerly homeless individuals' disharmonious relationships within the social context of PSH, consisting of staff members, other residents, and people in the surrounding community. The effects of the COVID‐19 pandemic and implications for PSH social services are discussed.
Muscle mitochondrial catalase expression prevents neuromuscular junction disruption, atrophy, and weakness in a mouse model of accelerated sarcopenia
Authors: Xu Hongyang , Ranjit Rojina , Richardson Arlan , Van Remmen Holly 
Published: September 2021

AbstractBackgroundOxidative stress and damage are associated with a number of ageing phenotypes, including age‐related loss of muscle mass and reduced contractile function (sarcopenia). Our group and others have reported loss of neuromuscular junction (NMJ) integrity and increased denervation as initiating factors in sarcopenia, leading to mitochondrial dysfunction, generation of reactive oxygen species and peroxides, and loss of muscle mass and weakness. Previous studies from our laboratory show that denervation‐induced skeletal muscle mitochondrial peroxide generation is highly correlated to muscle atrophy. Here, we directly test the impact of scavenging muscle mitochondrial hydrogen peroxide on the structure and function of the NMJ and muscle mass and function in a mouse model of denervation‐induced muscle atrophy CuZnSOD (Sod1−/− mice, Sod1KO).MethodsWhole‐body Sod1KO mice were crossed to mice with increased expression of human catalase (MCAT) targeted specifically to mitochondria in skeletal muscle (mMCAT mice) to determine the impact of reduced hydrogen peroxide levels on key targets of sarcopenia, including mitochondrial function, NMJ structure and function, and indices of muscle mass and function.ResultsFemale adult (~12‐month‐old) Sod1KO mice show a number of sarcopenia‐related phenotypes in skeletal muscle including reduced mitochondrial oxygen consumption and elevated reactive oxygen species generation, fragmentation, and loss of innervated NMJs (P < 0.05), a 30% reduction in muscle mass (P < 0.05), a 36% loss of force generation (P < 0.05), and a loss of exercise capacity (305 vs. 709 m in wild‐type mice, P < 0.05). Muscle from Sod1KO mice also shows a 35% reduction in sarco(endo)plasmic reticulum ATPase activity (P < 0.05), changes in the amount of calcium‐regulating proteins, and altered fibre‐type composition. In contrast, increased catalase expression in the mMCAT × Sod1KO mice completely prevents the mitochondrial and NMJ‐related phenotypes and maintains muscle mass and force generation. The reduction in exercise capacity is also partially inhibited (~35%, P < 0.05), and the loss of fibre cross‐sectional area is inhibited by ~50% (P < 0.05).ConclusionsTogether, these striking findings suggest that scavenging of mitochondrial peroxide generation by mMCAT expression efficiently prevents mitochondrial dysfunction and NMJ disruption associated with denervation‐induced atrophy and weakness, supporting mitochondrial H2O2 as an important effector of NMJ alterations that lead to phenotypes associated with sarcopenia.
Lawsone assisted preparation of carbon nanofibers for the selective detection of miRNA molecules
Authors: Sahtani Karima , Aykut Yakup , Tanik Nilay A 
Published: September 2021

AbstractBACKGROUNDmicroRNA (miRNA) molecules are considered as biomarkers and have promising future for early‐stage cancer diagnosis. Lawsone (Law) enriched carbon nanofibers (CNFs) were prepared via electrospinning process for the selective miRNA detection. Anti‐miRNA molecules were attached on the CNFs immobilized screen printed electrodes (SPE) and used for the detection of miRNA molecules by observing guanine oxidation signal via differential pulse voltammetry (DPV) method. Three different miRNA molecules including the target (miRNA), single‐base mismatched (SM.miRNA) and non‐complementary (NC.miRNA) were hybridized with the previously attached anti‐miRNA molecules (with guanine [PolyT(G)] and without guanine [PolyT(I)]) on the CNFs immobilized SPE surfaces.RESULTSGuanine oxidation signal decreased after the hybridization of miRNA with anti‐miRNA molecules on CNF immobilized SPE surfaces. Guanine oxidation signal was not detected on the PolyT(I) attached samples, but the existence of guanine oxidation signal was clear detected after the addition of miRNA molecules on the previously attached PolyT(I). Because no hybridization of anti‐miRNA molecules with the non‐complementary miRNA (NC.miRNA) molecules occurred, the guanine oxidation signal was not significantly decreased after the interaction of NC.miRNA with the attached PolyT(G). Since there was no hybridization between the NC.miRNA molecules with the attached PolyT(I) on the SPEs, weak guanine peaks were detected at PolyT(I)‐NC.miRNA samples as a result of the existence of residual NC.miRNA molecules on SPE after washing.CONCLUSIONSThe measurement results confirm the enhanced selectivity of the miRNA molecules by using CNFs with SPEs and the developed biosensory system could be used to detect the specific RNA molecules. © 2021 Society of Chemical Industry (SCI).
Genetic counseling in the time of COVID‐19: The Philippine experience with telegenetics
Authors: Tumulak Ma‐Am Joy R. , Pascua Angela V. , Jover Edbert Jasper M. , Guerbo Romer J. , Canoy Graciel Mae R. , Laurino Mercy Y. 
Published: September 2021

AbstractThe COVID‐19 pandemic poses a significant challenge to healthcare professionals and health systems around the world, most notably the disruption of its service delivery. The typical work setting for most genetic counselors (GCs) is in a clinic or hospital. However, during the COVID‐19 pandemic, to help prevent the further spread of the virus, clinics and hospitals have restricted non‐urgent in‐person delivery of healthcare services, including genetic counseling. Patients’ access to genetic counseling services has thus been limited, which prompted GCs in the country to utilize an alternative way to provide counseling through telegenetics. With the expansion of genetic services in the country, including the full implementation of expanded newborn screening, there is an increasing demand for genetic counseling and a growing need for telegenetics.
Design and synthesis of highly oxygenated furo[3,2‐c]pyran‐4‐ones and furo[3,2‐c]chromen‐4‐ones scaffold as anticancer and antimicrobial agents
Authors: Rani Suman , Kumar Devinder , Kamra Nisha , Thakral Sumit , Singh Ajeet , Sangwan Payare L. , Singh Shashank K. 
Published: September 2021

AbstractSynthesis of a number of highly oxygenated furo[3,2‐c]pyran/chromen‐4‐one has been accomplished by one‐pot reaction from easily available dehydroacetic acid/3‐acetyl‐4‐hydroxycoumarin or their chalcones and α‐bromoketones. All the synthesized molecules were characterized utilizing various spectroscopic techniques and screened for anticancer activity (in vitro) against three colon (HCT‐116, SW‐620, HT‐24), lung (A‐549), prostate‐(PC‐3), breast (MCF‐7) cell lines. Compounds 5a, 9d, 9f showed good activity against breast MCF‐7 cancer cell line having IC50 values 6.9, 2.8, 5.3 μM, respectively. Of these compounds, 9d showed better activity against prostate PC‐3 cell line with IC50 value 3.8 μM. The synthesized compounds were also studied for antibacterial activity (in vitro) using different strains of bacteria (Bacillus subtilis and Staphylococcus aureus – Gram‐positive and Escherichia coli, Pseudomonas aeruginosa – Gram negative) as well as fungal strains (Aspergillus niger and Candida albicans) using Norfloxacin and Fluconazole as antibacterial and antifungal standard drugs, respectively. The antimicrobial screening study showed that compound 9f exhibited promising activity against S. aureus and B. subtilis, while 5h showed excellent and 5i and 9b showed better activity against E. coli. Compounds 5d and 5e showed promising activity against P. aeruginosa. The compounds 5c–5e displayed excellent activity against C. albicans and A. niger than Fluconazole.
Noncontrast Cardiac Magnetic Resonance Imaging Predictors of Heart Failure Hospitalization in Heart Failure With Preserved Ejection Fraction
Authors: Kucukseymen Selcuk , Arafati Arghavan , Al‐Otaibi Talal , El‐Rewaidy Hossam , Fahmy Ahmed S. , Ngo Long H. , Nezafat Reza 
Published: September 2021

BackgroundHeart failure patients with preserved ejection fraction (HFpEF) are at increased risk of future hospitalization. Contrast agents are often contra‐indicated in HFpEF patients due to the high prevalence of concomitant kidney disease. Therefore, the prognostic value of a noncontrast cardiac magnetic resonance imaging (MRI) for HF‐hospitalization is important.PurposeTo develop and test an explainable machine learning (ML) model to investigate incremental value of noncontrast cardiac MRI for predicting HF‐hospitalization.Study TypeRetrospective.PopulationA total of 203 HFpEF patients (mean, 64 ± 12 years, 48% women) referred for cardiac MRI were randomly split into training validation (143 patients, ~70%) and test sets (60 patients, ~30%).Field strengthA 1.5 T, balanced steady‐state free precession (bSSFP) sequence.AssessmentTwo ML models were built based on the tree boosting technique and the eXtreme Gradient Boosting model (XGBoost): 1) basic clinical ML model using clinical and echocardiographic data and 2) cardiac MRI‐based ML model that included noncontrast cardiac MRI markers in addition to the basic model. The primary end point was defined as HF‐hospitalization.Statistical TestsML tool was used for advanced statistics, and the Elastic Net method for feature selection. Area under the receiver operating characteristic (ROC) curve (AUC) was compared between models using DeLong's test. To gain insight into the ML model, the SHapley Additive exPlanations (SHAP) method was leveraged. A P‐value <0.05 was considered statistically significant.ResultsDuring follow‐up (mean, 50 ± 39 months), 85 patients (42%) reached the end point. The cardiac MRI‐based ML model using the XGBoost algorithm provided a significantly superior prediction of HF‐hospitalization (AUC: 0.81) compared to the basic model (AUC: 0.64). The SHAP analysis revealed left atrium (LA) and right atrium (RV) strains as top imaging markers contributing to its performance with cutoff values of 17.5% and −15%, respectively.Data ConclusionsUsing an ML model, RV and LA strains measured in noncontrast cardiac MRI provide incremental value in predicting future hospitalization in HFpEF.Evidence Level3Technical EfficacyStage 2
The role of psychological distance in organizational responses to modern slavery risk in supply chains
Authors: Simpson Dayna , Segrave Marie , Quarshie Anne , Kach Andrew , Handfield Robert , Panas George , Moore Heather 
Published: September 2021

AbstractModern slavery is used to describe forms of coercive labor exploitation that affect more than 40 million persons globally. Such practices are difficult to identify given they exist in the informal economy, and involve vulnerable individuals. Addressing modern slavery by organizations requires awareness of its context and complexities. While corporations have increasingly sought to manage modern slavery risk in their supply chains, their understanding of what modern slavery is and what should be managed remains limited. We argue a key problem with firms’ efforts to manage modern slavery risk is that it is a psychologically distant concept for them. We apply construal level theory to explore how organizations’ psychological distance from modern slavery risk affects their management of risk. We interviewed purchasing executives at 41 global organizations in Australia, Finland, and the U.S and identified four approaches to managing modern slavery risk at different levels of psychological distance. We also identified that conflicts between organizations' approaches to risk and what they identify in their operating environment, precedes important construal shifts that help to improve organizational understanding of labor‐related risk. We highlight ways that organizations' understanding of modern slavery risk plays a role in their governance of such risk in supply chains.
Facile synthesis of noble metal decorated carbon nanostructure for SERS detection
Authors: Lai Kui , Xu Yinghao , Chen Jiahui , Gu Chenjie , Chen Da , Jiang Tao , Duan Tianli , Zhou Jun 
Published: September 2021

AbstractSurface enhanced Raman scattering (SERS) is a highly sensitive spectral analysis technique for ultra‐low trace molecule detection. Conventionally, noble metals like silver (Ag) and gold (Au) are used to prepare the SERS substrates; however, it usually requires careful experiment design and accurate process control, which defers their wide applications in practice. In this paper, a one‐step and low‐cost approach to prepare silver decorated porous carbon (Ag NPs/PCN) films for SERS application is reported. Experimentally, gelatin‐silver nitrate mixed solution was first spin‐coated on the Si substrates to form the thin films, and then, the substrates were carbonized at the different temperatures to obtain the Ag NPs/PCN films. The SERS performance of the Ag NPs/PCN films was evaluated by using the R6G as Raman reporter. Results reveal that the SERS activity is closely related to the spin‐coating rate and carbonization temperature. And detection limit of 10−8 M with the Raman enhancement factor of 1.64 × 106 (10−4‐M R6G) can be obtained on the most optimal substrate. Moreover, large‐scale mapping measurement further evidences that excellent Raman signal uniformity with the RSD of 6.93% also can be achieved.
Atmospheric cold plasma: a new approach to modify protein and lipid properties of myofibrillar protein isolate from hairtail (Trichiurus lepturus) fish
Authors: Hatab Shaimaa , Koddy John K , Miao Wenhua , Tang Lingling , Xu Huiqian , Deng Shanggui , Zheng Bin 
Published: September 2021

AbstractBACKGROUNDQuite recently, considerable attention has been paid to atmospheric cold plasma (ACP) as an eco‐friendly and highly efficient technology to modify the functional properties of foods. This study focuses on the effect of ACP on the myofibril protein and lipid quality of hairtail (Trichiurus lepturus) fish. In achieving this, the samples were treated with ACP at 50 kV for different times (30, 60, 120, 180, 240, 300 s).RESULTSThe findings indicated slight changes in peroxide value and thiobarbituric acid reactive substances in the samples treated with ACP. A significant increase (P < 0.05) in the surface hydrophobicity (from 131.71 ± 0.81 μg to 146. 34 ± 0.81 μg), turbidity (from 0.13 ± 0.001 to 0.27 ± 0.01), and water‐holding capacity (from 61.63% ± 5.7% to 64.86% ± 1.5%) were detected with treated samples.CONCLUSIONSWe conclude that ACP treatment induces marked changes in the protein and lipid properties of myofibril protein isolated from hairtail fish, which strengthen the gel formation of hairtail fish. © 2021 Society of Chemical Industry.
Electromembrane extraction in microfluidic formats
Authors: Hansen Frederik André , Petersen Nickolaj Jacob , Kutter Jörg P. , Pedersen‐Bjergaard Stig 
Published: September 2021

AbstractElectromembrane extraction is a microextraction technique where charged analytes are extracted across a supported liquid membrane and selectively isolated from the sample based on an electrical field. Since the introduction in 2006, there has been continuously increasing interest in electromembrane extraction, and currently close to 50 new articles are published per year. Electromembrane extraction can be performed in different technical configurations, based on standard laboratory glass vials or 96‐well plate systems, and applications are typically related to pharmaceutical, environmental, and food and beverages analysis. In addition to this, conceptual research has developed electromembrane extraction into different milli‐ and microfluidic formats. These are much more early‐stage activities, but applications among others related to organ‐on‐chip systems and smartphone detection indicate unique perspectives. To stimulate more research in this direction, the current article reviews the scientific literature on electromembrane extraction in milli‐ and microfluidic formats. About 20 original research articles have been published on this subject so far, and these are discussed critically in the following. Based on this and the authors own experiences with the topic, we discuss perspectives, challenges, and future research.