bluuebunny/crossref_metadata_2025_split huggingface dataset:
MiRNA-103b Downregulates ITGB3 and Mediates Apoptosis in Ex Vivo Stored Human Platelets
Authors: Dahiya Neetu , Atreya Chintamani 
Published: June 2021

Background:
Blood bank-stored human platelets are one of the life-saving transfusion
products to prevent bleeding in multiple clinical settings. In ex vivo storage, platelets undergo apoptosis
and it is highly desirable to prevent this process to preserve platelet quality. However, underlying
mechanisms of apoptosis are not well understood in stored platelets. Integrin beta 3 (ITGB3)
glycoprotein plays multiple roles in platelet physiological processes, and it was reported in other
cell types that downregulation of ITGB3 induces apoptosis. Small noncoding regulatory RNAs
known as microRNAs (miRNAs), some of which are abundant in platelets such as miR-103b that
belong to miR-103 family of miRNAs, known to play key roles in platelet functions both in vivo
and during storage; Cellular miR-103 downregulates certain genes in other cell types and promotes
apoptosis. However, whether miR-103b can target and downregulate ITGB3 in stored platelets and
such miRNA regulation promotes apoptosis is not known. Here, we tested this working hypothesis.

Objective:
Our objective of this study is to validate the abundance of miR-103b in stored platelets
and identify whether ITGB3 is a target of miR-103b for the downregulation and this interaction promotes
apoptosis.

Methods:
RT-qPCR validation of miR-103b was performed in 11 donor samples at 3 different storage
time points. In-silico analysis was performed to identify predicted targets of the miR-103b. The
miRNA and messenger RNA interactions were confirmed using different biochemical approaches
such as qRT-PCR, western blotting and, suppression of luciferase reporter gene expression by ectopic
expression of miR-103b in HeLa cells. Final validation of the functional role of miR-103b in
ITGB3 downregulation and resulting induction of apoptosis was assessed in stored platelets by
FACS analysis following ectopic expression of miR-103b.

Results:
Using the Target Scan Vert algorithm, we identified several integrin subunit-encoding mRNAs
as potential targets of miR-103b. While ITGB3 and ITGB6 were found to have two targeting
sites for miR-103b, since ITGB3 is known to play a role in apoptosis, we chose this for further validation
in this study. Ectopic expression of miR-103b decreased the luciferase reporter activity in
HeLa cells and decreased ITGB3 mRNA and protein levels in platelets, concomitant with an increase
in apoptosis.

Conclusion:
The results demonstrate that in stored platelets, miR-103b is highly expressed and can
interact with and downregulate ITGB3 and promote apoptosis in stored platelets.
MicroRNA Levels in Cervical Cancer Samples and Relationship with Lesion Grade and HPV Infection
Authors: Hoelzle Carolina R. , Arnoult Solène , Borém Cinthya R.M. , Ottone Mariana , de Magalhães Kênia C.S.F. , da Silva Istéfani L. , Simões Renata T. 
Published: June 2021

Background:
miR-21, miR-214 and miR-let-7a are three validated and well-known miRNAs.
miR-21 is described as an “oncomir” while miR-214 and miR-let-7a are described mainly as
tumor suppressors. The role of these miRNAs remains unclear in cervical cancer, an important malignancy
among women worldwide and responsible for many deaths every year.

Objective:
The objective of this study is to describe the expression profile of miR-21, miR-214 and
miR-let-7a in plasma and in cervical scraping from a control group and patients with different
grades of cervical lesions and invasive cervical cancer and correlate with HPV infection groups.

Methods:
Plasma and cervical scraping were submitted to DNA and RNA extraction. HPV detection
and typing were performed by conventional PCR followed by PAGE to amplicons interpretation.
The miRNA relative expression in plasma and cervical scraping samples was performed by real
time PCR using specific TaqMan probes.

Results:
miR-21 (p=0.0277) and miR-214 (p=0.0151) were up-regulated in cervical scraping samples
of invasive cervical cancer (ICC) group. However, miR-214 was also up-regulated in the LSIL
group (p=0.0062). Both miRNAs were not related to HPV infection. However, miR-let-7a was
higher in HPV positive plasma samples (p=0.0433) than in HPV negative plasma samples and the
correlation analysis confirmed the association between the levels of this miRNA with the presence
of HPV (p=0.0407; r=0.3029), but not with lesion grade (p>0.05).

Conclusion:
Our results suggest that miR-21 is related to cervical cancer progression and miR-214
appears to have an ambiguous role in cervical lesions. miR-let-7a may be upregulated at a systemic
level in patients with HPV infection.
INTELLECTUAL OPERATIONS DEVELOPMENT THROUGH LANGUAGE ACQUISITION AT A TECHNICAL UNIVERSITY
Authors: Ivanova Ludmila , Repkina Ludmila 
Published: June 2021

This paper deals with the idea of efficient intellectual operations development required for students to be successful at acquiring information via IT tools and learning a foreign language. The authors provide a list of functional soft skills referred to cogitative abilities. These operations ensure critical data selection during relevant engineering information search contained in digital engineering environment. Intellectual skills are rated in the course of instruction of graduates and undergraduates. Suggestions on skills formation and their further improvement on the basis of IT tools and functional approach are given.
A STUDY ON ALBANIAN STUDENTS' PERCEPTIONS OF THE ESP COURSES CONTRIBUTION TO THEIR ENGINEERING/ARCHITECTURE KNOWLEDGE ACQUISITION AND COMMUNICATION COMPETENCE
Authors: Alhasani Mirela 
Published: June 2021

Currently, soft skills mainly communication skills of engineering/architecture graduates have been considered as essential as their hard skills for the 21st century competitive global market. Given the cramped curricula of engineering/architecture programs, the research examines the beneficial impact that ESP undergraduate courses have on the tailored communication of engineering/architecture students at a case study analysis - EPOKA University in Tirana, Albania. In order to obtain feedback and unbiased perceptions on the tangible improvement in advanced English communication skills and disciplinary content knowledge acquisition, a questionnaire survey was conducted with the undergraduate students of EPOKA University at the Faculty of Architecture and Engineering upon successful completion of the two mandatory ESP courses at undergraduate level. A mixed method was utilized to collect the statistical data from questionnaires and the semi-structured interviews with students of engineering majors and architecture at the Faculty of Architecture and Engineering of EPOKA University who took English for Academic Purposes and Engineering English during 2018-2020 academic years. Research findings revealed the growing appreciation and high interest that students are placing into ESP courses as significant contributors to knowledge acquisition and soft skill betterment alongside the classic disciplinary courses of their disciplines. Key words; engineering/architecture major, ESP courses, needs analysis survey, EPOKA University
Ciencia, Tecnología, Ingeniería y Matemática STEM como Método de Enseñanza en Ingeniería
Authors: García-Carrillo Dixon Alirio , Anaya-Vejar Edgar Andrés , Medina-Delgado Byron 
Published: June 2021

La Ciencia, Tecnología, Ingeniería y Matemáticas STEM por sus siglas en ingles se utiliza para describir el estudio de cuatro de las grandes áreas del conocimiento científico, el STEM es una iniciativa que nace alrededor de los años 90, y fue creada por la agencia gubernamental de los estados unidos conocida como National Science Foundation NFC la cual se dedica a los avances en investigación y educación en todos los campos (no dedicados a la medicina), relacionados con la ciencia e ingeniería. La educación STEM es una estrategia que abarca las diferentes disciplinas de la investigación científica relacionada con las áreas del conocimiento declaradas por la Organización para la Cooperación y el Desarrollo Económico OCDE y enfoca sus esfuerzos en preparar a los profesionales del mañana, los cuales se encargaran de aportar la transferencia de conocimiento necesaria para los campos de acción futuros que se posicionan dentro de la llamada industria 4.0. El estudio de esta técnica o metodología se centra en la investigación de diferentes estrategias implementadas para llevar a cabo el aprendizaje efectivo de los alumnos en temas específicos de las ciencias y la ingeniería, como también en mostrar los campos de acción que cuentan con la inserción del STEM dentro de su currículo como herramienta metodológica a la hora de educar a los aprendices en temas relacionados con las Aplicaciones móviles, Arduino, Aeronáutica, Biotecnología, Ingeniería de Software, ingeniería de control, líneas de transmisión, robótica entre otras, demostrando cuan beneficioso ha sido para el reclutamiento de nuevos estudiantes en campos de carreras profesionales científicas y la adquisición de competencias y habilidades específicas de los estudiantes
Enfermera-matrona: beneficios, competencias e intervenciones
Authors: Castiblanco-Montañez Ruth Alexandra , Berruecos-Prada Diana Camila , Calderón-Rivas Esmeralda María , Guayacundo-Aldana Mary Julieth , Mancera-García Ruby Magdaly , Rodríguez-Ramírez Karen Sofía 
Published: June 2021

Introducción: La enfermera permite a las gestantes tener autonomía en el embarazo y parto, pero no es reconocido actualmente; sin embargo la Resolución 3280 de 2018 define los lineamientos técnicos y operativos de la Ruta Integral de Atención en Salud para la Población Materno Perinatal y establece las directrices para su operación, destacando la participación del profesional de enfermería en la consulta preconcepcional y controles prenatales. Objetivo: Reconocer los beneficios de la atención de las enfermeras durante la gestación y el trabajo de parto reportados en la literatura. Metodología: Revisión integrativa de la literatura con un período de búsqueda de Febrero-Mayo del 2020 en EBSCO, Pubmed, LILACS, Cuiden y Google académico con términos DeCS-MeSH: Matronas, complicaciones, parto, embarazo, cuidados de enfermería y puerperio; operadores booleanos AND-OR. Se obtuvo 44.966 artículos en inglés, español y portugués agrupados según su título, resumen, texto completo, nivel de evidencia y grado de recomendación, obteniendo 33 artículos para realizar lectura crítica. Se cumplen las consideraciones éticas para estudios documentales. Resultados: A través de un análisis de contenido se construyeron 4 temáticas: Beneficios de la asistencia gestacional y de parto por la enfermera, competencias e intervenciones de la enfermera en la atención integral de la mujer gestante, Consideraciones de riesgo en la asistencia de la gestación y el parto por enfermeras y Retos de la enfermera en la atención durante el embarazo, parto y puerperio. Conclusión: La asistencia de la enfermera en gestaciones de bajo riesgo aporta múltiples beneficios a la mujer, reduce el tiempo de nacimiento y el dolor, posibilita la identificación de riesgos de manera oportuna.
La comodidad para el paciente crónico desde el sentir y el hacer
Authors: Cardozo-Garcia Yadira , Salazar-Maya Angela Maria , Escobar-Ciro Carmen Liliana 
Published: June 2021

Objetivo: Describir el significado de comodidad para los pacientes con enfermedad crónica que se encuentran hospitalizados en una institución de tercer nivel de complejidad de la ciudad de Medellín. Materiales y métodos: Estudio cualitativo, enfoque etnográfico. Se realizaron quince entrevistas y cuarenta horas de observación participante. Los participantes fueron los pacientes con enfermedad crónica hospitalizados en el servicio de medicina interna en una institución de la ciudad de Medellín (Colombia). Para el análisis se realizó lectura y relectura de los relatos, mediante la codificación se identificaron los conceptos para categorizarlos, relacionando las categorías con sus subcategorías. Resultados: El significado que construye el paciente con enfermedad crónica acerca de la comodidad es un concepto muy amplio que considera aspectos del entorno, físicos, sociales, emocionales, socioculturales y que deben ser considerados durante la atención para lograr el bienestar del paciente y del cuidador durante el tiempo de hospitalización. Conclusiones: La comodidad es un componente del cuidado que debe ser suplido por la enfermera, es importante comprender las formas y los medios de brindar comodidad a los pacientes hospitalizados con el fin de lograr el mayor estado de bienestar de los pacientes durante la estancia hospitalaria. A su vez las instituciones hospitalarias deben escuchar la voz del paciente con el fin de hacer las adecuaciones pertinentes para que la experiencia de hospitalización sea grata y lo menos traumática posible para los pacientes con enfermedad crónica.
Implications of Employee Satisfaction and Work-Life Balance in Accounting Firms
Authors: Khavis Joshua A. , Krishnan Jagan , Tipton Colin 
Published: June 2021

SUMMARY
This article summarizes and reflects on the implications of the published study “Employee Satisfaction and Work-Life Balance in Accounting Firms and Audit Quality” (Khavis and Krishnan 2021), which examines the link between employee satisfaction, work-life balance, and audit quality within accounting firms. The study uses employee-level reviews from Glassdoor.com to examine how accounting firms are perceived by their employees, and tests whether accounting firms' workplace characteristics explain employee satisfaction and audit quality. We briefly summarize the findings of this study and highlight relevant practical implications related to audit quality, employee recruitment, and employee retention, which may interest practitioners, regulators, and academics.
Main forestry and management indices of pine (Pinus sylvestris L.) stands involving beech (Fagus sylvatica L.) in composition of Ukrainian Roztochchia
Authors: Yuriy Myklush , Stepan Myklush , Serhii Havryliuk , Volodymyr Savchyn 
Published: June 2021

Abstract
As of 2019, the total area of forest stands with the varying proportion of Scots pine (Pinus sylvestris L.) and beech (Fagus sylvatica L.) in composition on the Ukrainian Roztochchia was 11,251.3 ha and the area of pine stands involving European beech in composition was 6879.5 ha. Pine stands involving beech in composition are formed on the fresh, moist and boggy relatively pure and relatively rich soil condition. Pine forest stands (average share of pine exceeds 56%), which include beech in composition occupy more than 80% of the total area of interest. Mature and overmature forest stands dominate most of the landscape. More than 85% of stands are characterized by an average relative completeness.
Undergrowth of deciduous tree species, which is mainly formed by European beech or hornbeam (Carpinus betulus L.) and rarely by common oak (Quercus robur L.) or sycamore maple (Acer pseudoplatanus), is available under a canopy of middle-aged, ripening and mature stands. The study points to the need to rethink forest management approaches and eventually to use such methods of forest cuttings that ensure natural regeneration not only of a European beech and common oak, but primarily of a pine. The practice of creating pine plantations in such conditions favourable for its growth and development should be used only in those cases when it is impossible to achieve natural regeneration of pine.
Trends in forest fuel accumulation in pine forests of Kyiv Polissya in Ukraine
Authors: Hurzhii Roman V. , Yavorovskyi Petro P. , Sydorenko Serhii Н. , Levchenko Valery B. , Tyshchenko Olexandr M. , Tertyshnyi Anatolii P. , Yakubenko Borys Ye. 
Published: June 2021

Abstract
At present, forest fire research is becoming especially relevant in Ukraine. This study examines patterns of forest fuel accumulation in pine (Pinus sylvestris L.) stands that grow in different soil conditions with different pine stand structure. To estimate the load of forest fuel of different fractions, a combined methodology was used: the weighing method and the FIREMON (fuel load estimation) method.
It was found that increase in surface forest fuel loads is not directly proportional to forest stands’ age. Fractional size distribution, capacity and loads of forest fuel depend on several factors, among which the greatest role is played by forestry characteristics of the pine stand. It was determined that in the forest site conditions of type C (fairly rich soils) in Kyiv Polissya, the share of forest litter compared to pine stands that grow in poor soil conditions (A) is smaller, ranging from 41% to 76% of the total forest fuel load. The mass proportion of the duff layer varies from 15% in young forest stands to 43% in mature stands. It was established that changes in forest fuel fractions for 1, 10, 100 and 1000 hours varied insignificantly with age rate. The share of substratum woody debris of 10 and 100 hours was insignificant and depended more on the forestry treatment regime on these sites. The mass proportion of coarse woody debris (1000 hours) was also insignificant, varying from 0% to 5.9% of the total load of surface fuel.
Distribution and natural regeneration of Tilia cordata Mill. in Ukrainian plain forests in a changing climate
Authors: Rumiantsev Maksym , Luk’yanets Volodymyr , Kobets Oleksii , Obolonyk Iryna , Tarnopilska Oksana , Poznіakova Svitlana , Musienko Sergiy , Tupchii Olha 
Published: June 2021

Abstract
The aim of this study was to assess the current state of small-leaved lime stands under climate change, as well as to identify optimal conditions for the occurrence and further growth of natural lime regeneration under the stand canopy to grow productive and sustainable mixed stands. The areas of small-leaved lime (Tilia cordata Mill.) stands in plain Ukrainian forests and mixed stands with English oak (Quercus robur L.) were defined according to reference materials (as of 2016). Growth and liveability peculiarities of natural regeneration of lime under the canopy of oak-lime stands in climate change were identified. The main stand characteristics contributing to maximal appearance and further development of advance growth of lime, such as composition, age and relative density of stocking, were determined. It was found that the lime stands of vegetative origin occupy the largest part of the lime forests’ area, being less resilient and productive than those originated from seeds. Small-leaved lime is the best associated species in mixed oak stands. The presence of lime improves health, assortment composition, and productivity of oak stands and increases their resistance against pests and diseases. In the future, lime can play an important role in the adaptation of forests to climate change due to its biological and forest properties and adaptability to a sustainable existence in a relatively wide range of environmental conditions. A summary of the studies complements a system of knowledge about the current state of lime stands as well as peculiarities of their regeneration and growth. The data obtained can be used as a theoretical base for forest management to promote natural regeneration and maintain the sustainability of these forests.
Scots pine (Pinus sylvestris L.) reaction to climate change in the provenance tests in the north of the Russian plain
Authors: Nakvasina Elena N. , Prozherina Nadezhda A. 
Published: June 2021

Abstract
Species with continuous distribution area will be impacted by climate change in different ways. That is related to the population’s geographical position and climate features of the population formation. Short-term response of Scots pine (Pinus sylvestris L.) was studied with taken into consideration intraspecies features of populations. Provenance tests in the Arkhangelsk (62.60 N, 39.98 E) and Vologda (62.60 N, 39.98E) regions located in the north of the Russian Plain were used. Provenances collection (23 provenances from the northern, middle, and southern taiga subzones and mixed forest zone) from areas with different climate characteristics was considered. Clinal variability and a reaction norm of vegetative and generative response to various levels of temperature change and seed transfer were studied. Average actual height and diameter values for 31-year provenances and calculated values for provenances were compared using ‘latitudinal growth coefficient’ proposed by I.V. Volosevich (1984) for the north of the Russian Plain. Provenance reproductive ability response was assessed using seed-bearing trees’ numbers in provenances of the 1st class of age. Pine growing in the north of the Russian Plain would respond to warming by productivity increasing more significantly than pine growing in the south. Response of pine from the northern and middle taiga subzones on climate warming can be expected on 1.01 m and 1.12 cm to temperature rise by 100°C for height and diameter, and 0.85 m and 0.93 cm for seeds transfer to 1 degree of northern latitude to southward. Probable reaction norm for pine reproduction potential under temperature change by 100°C of the sum of the temperatures above 10ºС and seed transfer by 1 degree of northern latitude can be expected about 6%.
The attempt to assess the fire risk of non-forest terrestrial ecosystems of Biebrza National Park – A case study
Authors: Szczygieł Ryszard , Kwiatkowski Mirosław , Kołakowski Bartłomiej 
Published: June 2021

Abstract
On 19th April, the largest wildfire of the entire history of Biebrza National Park broke out and consumed 5526 ha, mostly overdried grassland with sedges and reeds of Biebrza marshes. The very rapid spread of the fire in the open space, with the blowing wind changing directions and the inaccessibility of the area for fire-fighting vehicles were the main reasons of this third largest wildfire in Poland, after the tragic forest fires in Kuźnia Raciborska (9060 ha burnt) and Potrzebowice (5600 ha burnt) in the memorable year of 1992. After this event, activities were undertaken to develop a fire protection plan for the Biebrza National Park.
It took into account an innovative approach during the analysis of the existing fire risk, primarily regarding the non-forest terrestrial ecosystems composed of herbaceous vegetation, which constitutes as much as 61.2% of the park’s area, and to propose protection methods adequate to the threat. The work was completed in the framework of the project entitled ‘Development of the method for assessing the fire risk of non-forest ecosystems and the principles of fire protection for the Biebrza National Park – stage I,’ commissioned for the Forest Research Institute by the Biebrza National Park, financed by the State Forests from the forest fund, in accordance with the contract EZ.0290.1.24.2020. The article presents a preliminary method of classifying the fire risk of non-forest ecosystems, considering the occurrence of the fires in the Biebrza National Park in the years 2007–2020 and the type of vegetation burnt.
This method, after supplementary field tests planned in 2021, will enable evaluation of the fire risk, which shall be a premise for the development of a fire protection plan.