bluuebunny/crossref_metadata_2025_split huggingface dataset:
Devising a Selection Strategy for the Jamunapari Goat for Improving Lactation Performance
Authors: Dige Mahesh Shivanand , Rout P. K. , Bhusan S. , Gowane G. R. 
Published: September 2021

Abstract
This study aims to evaluate the genetic potential of the Jamunapari goat and formulate a new selection strategy for improving the lactation traits. The data set included 4049 phenotypic records for lifetime milk yield at 90 days (MY90) and 140 days (MY140), total milk yield (TMY), and lactation length (LL) obtained from the progeny of 83 sires and 1643 dams between 1990 and 2019. Animal model employing average information restricted maximum likelihood (AIREML) was used to estimate genetic parameters for milk yield traits and LL. The direct additive heritability estimates for lifetime lactation traits, that used repeatability model were 0.10 ± 0.03, 0.08 ± 0.03 and 0.12 ± 0.02 for MY90, MY140 and TMY, respectively, while it was low for LL (0.06 ± 0.02). The repeatability estimates were moderate ranging from 0.17 to 0.22 for milk yield traits and LL, indicating persistent performance over the parities. Animal permanent environment influence (c2) was significant in milk yield attributes, whereas additive maternal genetic effects were absent. As the early selection criteria based on first parity records is essential, we analysed the data for the first parity separately and obtained moderate h2 estimates viz. 0.26 ± 0.05, 0.26 ± 0.06 and 0.25 ± 0.06 for MY90, MY140 and TMY, respectively. These estimates augurs further positive scope of selection in Jamunapari goats for higher milk yield. High and positive genetic correlation of MY90 with MY140 (0.97 ± 0.01) and TMY (0.91 ± 0.05) revealed the scope of using MY90 as the selection criterion.Based on these results, we recommend use of MY90 as a single trait selection criterion for genetic improvement of all lactation traits in Jamunapari goat.
Wiener Gain and Deep Neural Networks: A Well-Balanced Pair For Speech Enhancement
Authors: Ribas Dayana , Miguel Antonio , Ortega Alfonso , Lleida Eduardo 
Published: September 2021

Abstract
This paper proposes a Deep Neural Network (DNN)-based Wiener gain estimator for speech enhancement. The proposal is in the framework of the classical spectral-domain speech enhancement algorithms. In this case, we used the Optimal Modified Log-Spectral Amplitude (OMLSA), but consider that this proposal could fit many alternative speech estimation algorithms. We determined the best usage of the DNN approach at learning a robust instance of the Wiener gain estimator according to the characteristics of the SNR estimation and the gain function. To design a DNN architecture adjusted for the speech enhancement task, we study various configuration issues frequently used in DNN-based solutions, including speech representations, residual connections, and causal vs. non-causal designs. Thus, we provide conclusions for the use of DNN architectures with the enhancement purpose. Experiments show that the proposal provides results on the state-of-the-art. But beyond the objective quality measures, there are examples of noisy vs. enhanced speech available for listening to demonstrate in practice the skills of the method in real audio.
ErbB2 Expression on Left Ventricular Epicardial Endothelial Cells and CD105+ Cells is Decreased in Patients with Diabetes Mellitus.
Authors: deKay Joanne T. , Carver Joshua , Shevenell Bailey , Kosta Angela M. , Tsibulnikov Sergey , Certo Emily , Sawyer Douglas B. , Ryzhov Sergey , Robich Michael P. 
Published: September 2021

Abstract
Background

We investigated the cell surface expression of ErbB receptors on left ventricular (LV) epicardial endothelial cells and CD105+ cells obtained from cardiac biopsies of patients undergoing coronary artery bypass grafting surgery (CABG).
Methods

Endothelial cells and CD105+ non-endothelial cells were freshly isolated from LV epicardial biopsies obtained from 15 subjects with diabetes mellitus (DM) and 8 controls. The expression of ErbB recepotrs was examined using multiparametric flow cytometry. Human microvascular endothelial cells (HMEC-1) and LV epicardial CD105+ non-endothelial cells were used to determine the effect of high glucose on ADAM10-dependent cleavage of ErbB receptors.
Results

We found that diabetes mellitus (DM) and high levels of hemoglobin A1C are associated with reduced expression of ErbB2 on both endothelial cells and CD105+ non-endothelial cells. To determine if the expression of ErbB2 receptors is regulated by glucose levels, we examined the effect of high glucose in HMEC-1 and LV epicardial CD105+ non-endothelial cells, using a novel flow cytometric approach to simultaneously determine the total level, cell surface expression, and phosphorylation of ErbB2. Incubation of cells in the presence of 25 mM D-glucose resulted in decreased cell surface expression of ErbB2. We also found high expression of a disintegrin and metalloproteinase domain-containing protein 10 (ADAM10) on both endothelial cells and CD105+ non-endothelial cells. Inhibition of ADAM10 prevented the high glucose-dependent decrease in the cell surface expression of ErbB2.
Conclusions

We suggest that high glucose depresses ErbB receptor signaling in endothelial cells and cardiac progenitor cells via the promotion of ADAM10-dependent cleavage of ErbB2 at the cell surface, thus contributing to vascular dysfunction and adverse remodeling seen in diabetic patients.
A Cross-sectional Study of Mental Health, Posttraumatic Stress Symptoms and Post Exposure Changes in Norwegian Ambulance Personnel
Authors: Reid Bjørn Ole , Næss-Pleym Lars Eide , Bakkelund Karin Elvenes , Dale Jostein , Uleberg Oddvar , Nordstrand Andreas Espetvedt 
Published: September 2021

Abstract
Background Posttraumatic Stress Disorder (PTSD) has been shown to be elevated among first responders (Emergency Medical Services, fire service, police force) compared to the general population. Examining the prevalence of mental health issues in a work force with an elevated occupational risk is fundamental towards ensuring their wellbeing and implementing safeguard measures. The goal of this study is therefore to report the prevalence of depression, anxiety, posttraumatic development, and PTSD in Norwegian ambulance personnel. Methods This study is a cross-sectional, anonymous, web-based survey (Questback®), performed among operative personnel employed in the Emergency Medical Services in the Regional Health Trust of Central Norway between 18. February and 9. April 2021. The study was sent to 1052 eligible participants. Questions reported demographic data, a traumatic events exposure index, Patient Health Questionnaire-9 (Depression), Generalized Anxiety Disorder-7 scale, Posttraumatic symptom scale (PTSD) and Posttraumatic change scale. Results The response rate in this study was 45.5 % (n=479/1052). The mean age of respondents was 37.1 years (std. 11.1) and 52.8 % (n=253) were male. Of the respondents, 80.6% (n=386) were married or had a partner, and 91.6% (n=439) reported having access to a peer support programme, with 34.9% (n=167) reporting that they had utilized peer support. In this study, 5 % (n=24) showed a prevalence of manifest posttraumatic stress disorder symptoms, while 8.6 % (n=41) reported moderate to severe depression and 2.9 % (n=14) presented moderate to severe symptoms of general anxiety. Of the respondents, 77.2 % (n=370) reported personal growth because of their work experiences. Conclusions This study indicates that Norwegian ambulance personnel report a prevalence of posttraumatic stress symptoms and depression, which is slightly higher for men, and lower for the female proportion in this study, when compared to an adult Norwegian population. The vast majority of respondents reported personal growth because of their work experience, and both the degree of peer support and having a partner seem to influence levels of posttraumatic stress and -development.
MCUR1 Promotes Osteosarcoma Cell Growth Through AKT/p53 Pathway
Authors: Wang Yaya , Tan Rui , Hou Zhenghui , Tian Yiyuan , Chen Yafan , Zhang Hongxin , Yuan Peng 
Published: September 2021

Abstract
Background: Osteosarcoma (OS) is one of the most aggressive malignant bone tumor worldwide. This study focuses on investigating the mechanism underlying the mitochondrial calcium uniporter regulator 1 (MCUR1)-mediated osteosarcoma (OS) cell growth. Methods: A total of 49 patients diagnosed as OS in our hospital were included in the current study. The expression of MCUR1 in human OS tissues and various OS cell lines was detected by immunohistochemical staining (IHC) and western blot analysis, respectively. The effect of MCUR1 on AKT/p53 pathway and its correlation with miR-506-3p were investigated by a series of experiments including MTS, QRT-PCR, Western blot and IHC. Results: Our data showed that MCUR1 was highly overexpressed in OS tumor tissues compared with the para-carcinoma tissues (P<0.01). The Kaplan-Meier analysis showed that high expression level of MCUR1 was linked with poor prognosis of OS patients. Additionally, knockdown of MCUR1 enhanced OS cell apoptosis and decreased the growth of OS cells compared with the corresponding controls (P<0.05). Meanwhile, the expression level of p-AKT was decreased, whereas the protein expression level of p53 was increased in OS cells with MCUR1 downregulation. We also found that the IHC scores of MCUR1 were inversely correlated with that of p53 (r = -0.304, P=0.034). Likewise, the expression of MCUR1 and miR-506-3p in OS tissues were also inversely correlated (r=-0.304, P=0.034). Conclusions: MCUR1 is overexpressed in OS cells and its expression is regulated by miR-506-3p. MCUR1 facilitates the progression of OS through activating AKT/p53 pathway. The data in the current study suggests that MCUR1 may serve as a new target for the diagnosis and treatment of OS.
T7-lac Promoter Vectors Derepression Caused by Plant-Derived Growth Media Can Lead to Serious Expression Problems: A Systematic Evaluation.
Authors: Krefft Daria , Prusinowski Maciej , Maciszka Paulina , Skokowska Aleksandra , Zebrowska Joanna , Skowron Piotr M 
Published: September 2021

Abstract
BackgroundThe widespread usage of protein expression systems in Escherichia coli (E. coli) is a workhorse of molecular biology research and practical applications in biotechnology industry, including pharmaceutical drugs production. Various factors can highly affect successful clones construction and their stable maintenance as well as obtained biosynthesis levels. These include correct selection of recombinant hosts, expression systems, promoters regulation, repression level at uninduced state, growth temperature, codon usage, codon context, mRNA secondary structure, translation kinetics and chaperons presence/absence, among others. However, the optimization of the growth media compositions is often overlooked. We systematically evaluate this factor, which can have dramatic effect on biosynthesis of recombinant proteins, especially those, which are toxic to a recombinant host. Results Commonly used animal tissue- and plant-based media were evaluated using a series of clones in pET vector, containing expressed ORFs with wide spectrum of toxicity to the recombinant E. coli: (i) gfpuv (nontoxic); (ii) tp84_28 – coding thermophilic endolysin (moderately toxic) and (iii) tthHB27IRM – coding for thermophilic restriction endonuclease-methyltransferase (REase-MTase) (very toxic). The use of plant-derived peptones (soy peptone and wheat extract) in culture media causes leakage of the T7-lac expression system. We show, that the presence of raffinose and stachyose (galactoside derivatives) in those peptones causes premature and uncontrolled induction of gene expression, which affects the course of cultures, clones stability and biosynthesis levels.Conclusions The use of plant-derived peptones in culture media when using the T7-lac hybrid promoter expression systems, such as Tabor-Studier, can lead to uncontrolled production of a recombinant protein. These conclusions also extend to other, lac operator-controlled promoters. In the case of proteins toxic to a recombinant host, this can result in the expression vector and/or cloned gene mutations, deletions, host’s death or highly decreased expression levels. This phenomenon is caused by certain saccharides content in plant peptones, some of which (galactosides) may act as T7-lac promoter inducers by theirinteraction with Lac repressors. Thus, when attempting to overexpress toxic proteins, it is recommended to either not use plant-derived media or use them with caution and to pilot-scale evaluate the derepression effect on the case-by-case basis.
Trends in Obesity and Metabolic Status Among a Health Check-Up Population in Beijing and Hunan Between 2012 and 2020
Authors: Li Ying , Yang Lin , Yin Lu , Liu Qingqi , Wang Yaqin , Yang Pingting , Wang Jiangang , Chen Zhiheng , Li Xiaohui , Yang Qinyu , He Yongmei , Huang Xin 
Published: September 2021

Abstract
Background: Previous cross-sectional studies have reported the prevalence of obesity and metabolic status in China. However, the trend of change in obesity and metabolic status, especially in different sex and age groups are lacking. Methods: In a series cross-sectional study, data on 256,782 participants surveyed between 2014 and 2020 in Beijing and 697,170 participants surveyed between 2012 and 2020 in Hunan were analyzed. Anthropometrics, blood pressure measurements, and blood tests were performed according to standard protocols. Trends in obesity and metabolic status were evaluated using the Joinpoint software to estimate annual percentage changes in slopes.Results: Based on age- and sex-standardized values, the mean BMI values in Beijing and Hunan participants were 23.94 (95%CI: 23.93, 23.95) and 23.68 (95%CI: 23.67, 23.69) kg/m2, respectively. Between 2014-2020, the overall obesity prevalence among Beijing participants increased from 12.70% (95%CI: 12.17%, 13.23%) to 14.33% (95% CI: 13.97%, 14.70%) (P=0.009), mainly derived by the 20-39 and 40-59 age groups. Moreover, the prevalence of metabolically healthy obese significantly increased from 2.07% (95%CI: 1.84%, 2.30%) to 4.33% (95% CI: 4.13%, 4.53%) in Beijing. Between 2012-2020, no significant trend in obesity was found among overall Hunan participants, but the prevalence of metabolically unhealthy obese significantly increased from 5.36% (95% CI: 5.18%, 5.54%) to 7.35% (95% CI: 7.11%, 7.58%), mainly derived by the 20-39 and 40-59 age groups.Conclusions: The trends in obesity and metabolic status were different between Hunan and Beijing. National weight control plan is needed in China, with a particular focus on young and middle-aged population.
Forecasting Daily Emergency Department Arrivals Using High-Dimensional Multivariate Data: A Feature Selection Approach
Authors: Tuominen Jalmari , Lomio Francesco , Oksala Niku , Palomäki Ari , Peltonen Jaakko , Huttunen Heikki , Roine Antti 
Published: September 2021

Abstract
Background and Objective Emergency Department (ED) overcrowding is a chronic international issue that is associated with adverse treatment outcomes. Accurate forecasts of future service demand would enable intelligent resource allocation that could alleviate the problem. There has been continued academic interest in ED forecasting but the number of used explanatory variables has been low, limited mainly to calendar and weather variables. In this study we investigate whether predictive accuracy of next day arrivals could be enhanced using high number of potentially relevant explanatory variables and document two feature selection processes that aim to identify which subset of variables is associated with number of next day arrivals.Methods We extracted numbers of total daily arrivals from Tampere University Hospital ED between the time period of June 1, 2015 and June 19, 2019. 158 potential explanatory variables were collected from multiple data sources consisting not only of weather and calendar variables but also an extensive list of local public events, numbers of website visits to two hospital domains, numbers of available hospital beds in 33 local hospitals or health centres and Google trends searches for the ED. We used two feature selection processes: Simulated Annealing (SA) and Floating Search (FS) with Recursive Least Squares (RLS) and Least Mean Squares (LMS). Performance of these approaches was compared against autoregressive integrated moving average (ARIMA), regression with ARIMA errors (ARIMAX) and Random Forest (RF). Mean Absolute Percentage Error (MAPE) was used as the main error metric.Results Calendar variables, load of secondary care facilities and local public events were dominant in the identified predictive features. RLS-SA and RLS-FA provided slightly better accuracy compared ARIMA. ARIMAX was the most accurate model but the difference between RLS-SA and RLS-FA was not statistically significant.Conclusions Our study provides new insight into potential underlying factors associated with number of next day presentations. It also suggests that predictive accuracy of next day arrivals can be increased using high-dimensional feature selection approach when compared to both univariate and nonfiltered high-dimensional approach. However, outperforming ARIMAX remains a challenge when working with daily data. Future work should focus on enhancing the feature selection mechanism, investigating its applicability to other domains and in identifying other potentially relevant explanatory variables.
Stunting and Associated Factors Among 6-23 Month Old Children in Drought Vulnerable Kebeles of Demba Gofa District, Southern Ethiopia
Authors: Tringo Tadele Tuba , Chalite Gebremedhin Chameno , Urugo Markos Makiso , Liben Fitsum Endale 
Published: September 2021

Abstract
Background

Stunting is impaired linear growth of children that they experience it in the first 1000 days after conception and indication of chronic malnutrition. It is caused by poor maternal nutrition and other interrelated factors.
Objective

This study conducted to assess the magnitude and associated factors of stunting among 6–23 month old children in drought vulnerable kebeles of Demba Gofa district.
Methodology:

A cross-sectional study with stratified multistage sampling was conducted. Semi-structured questionnaire and anthropometric measurements were used to collect the data. Bivariate and multivariable logistic regression models were fitted using SPSS 20.0 for Windows. Adjusted odds ratios (AOR) with 95% confidence intervals (CI) were used to measure the strength of the association between dependent and associated variables at a p value < 0.05.
Result

From the total study population (362), 79 (21.82%) of the children were stunted. Household dietary diversity [AOR = 0.45, 95% CI, 0.78, 0.966], exclusive breast feeding [AOR = 3.54, 95% CI, 1.33, 9.41], early initiation of complementary feeding [AOR = 0.08, 95% CI, 0.044–0.174] and not feeding animal source food during complementary feeding [AOR = 0.061, 95% CI, 0.016–0.226] were significantly associated with child stunting.
Conclusions

The extent of stunting in the study area is relatively lower than national and regional average. However, based on the findings awareness creation on exclusive breast feeding, complementary feeding and dietary diversification strategies suggested to lessening the problem.
Development of In-Vitro Regeneration Protocol From Seedling Explants of Populus Gamblei Dode: A Difficult to Propagate Endemic Tree Species of Eastern Himalayas
Authors: Chandra Puran , Thakur Ajay , Mohapatra K P , Mehra T S , Kunwar Arti , Chauhan Monika 
Published: September 2021

Abstract
This is the first report of in-vitro regeneration of Populus gamblei, an endemic and rare tree of montane to subtropical forest of Eastern Himalayas. Nodal segment from coppice shoot of young headed back seedling resulted in 100 % sprouting in MS + 1.0 mg L-1 BAP. Each micro shoot proliferated and gave 7.06 micro shoots on MS + 0.50 mg L-1 BAP + 0.10 mg L-1 IBA. In vitro multiplied shoots produced 100 % rooting in ½ MS + 0.5 mg L-1 NAA. In-vitro regenerated plants recorded 80 percent survival during hardening.
The Perception of Feedback and Strengthening the Feedback Process. A Qualitative Thematic Analysis Among Residents and Supervisors in Anaesthesia and Intensive Care
Authors: Tigerschiöld Stephanie , Darvish Bijan , Falk Ann-Charlotte , Nordquist Jonas 
Published: September 2021

Abstract
BackgroundMedical education worldwide is currently undergoing a shift through the implementation of Competency Based Medical Education (CMBE). Feedback is a key factor for implementation of CBME as well as an important element in the process of continuous learning for healthcare professionals. We have investigated the perception of feedback and how to strengthen feedback processes among residents and supervisors in five anaesthesia and intensive care departments in Stockholm, Sweden. MethodA survey was sent out to 101 residents and 168 supervisors in anaesthesia and intensive care in Stockholm. The data was analysed using thematic analysis. Results Our results show that both residents and supervisors had similar mental models of feedback. Feedback was considered to enhance teamwork, create positive reinforcement and support professional development. Our material provides insights to factors that inhibit feedback, such as psychological unsafety, lack of feedback culture and shortage of knowledge regarding feedback processes. ConclusionAn organizational and systematic approach is needed to empower the use of feedback within the anaesthesia and critical care environment. Establishing good learning environments and strong feedback cultures is an investment for the future of medical education.
A Comparative Transcriptome Analysis Reveals New Insights into Pre-Harvest Sprouting (PHS) in Wheat
Authors: Li Xingyan , Qiao Hongyu , Wang Zeng , Han Bing , Xing Yanping , Yang Yan 
Published: September 2021

Abstract
Background: pre-harvest sprouting (PHS) is a significant cause of yield loss in cereal crops, and is an important topic of study for the improvement of wheat quality. Many studies have focused on PHS in wheat during the last 10 years, especially on the involvement of abscisic acid (ABA) in PHS, however, a lot remained unknown about this topic.Results: In this study, a PHS resistant line was isolated from an ethylmethane sulfonate (EMS) mutant population derived from the wheat cultivar ‘Long 13-3778’, namely ‘LQ18’. The mutant line LQ18 showed highly significant resistance to PHS compared with the wild-type. Transcriptome sequencing was conducted to determine the differences between the LQ18 mutant and the wild-type at the level of gene expression. The results showed no conclusive evidence that the ABA biosynthesis and signaling pathways contribute to the differences in PHS between the mutant and the wild-type, and some genes and their alleles associated with PHS tolerance showed differential expression between the mutant and wild-type lines. The most interesting result of this study was that the expression levels of the chitinase family genes showed significant differences between the mutant and the wild-type as determined by GO enrichment analysis, and a subsequent analysis of differential expression profiling of the chitinase genes led to the same conclusion.Conclusions: Transcriptomic analysis in this study have revealed the global transcriptome profiles of the PHS sensitive wheat cultivar ‘Long 13-3778’ and its PHS resistant mutants. Furthermore, this study has proposed a possible explanation of the connection between PHS and the chitinase family for the first time, which added to our understanding of PHS and seed dormancy in common wheat.