P–073 High-intensity interval training modulates the effects of hypertension on inflammatory mediators in testis in adult spontaneously hypertensive rats
Authors: Giometti I , Veras A , Pacagnelli F , Schaffer L , Oliveira M , Nespolo R , Santos A , Souza F , Castilho C , Favareto A P , Mendes L , Teixeira G 
Published: August 2021

Abstract

Study question
Is the high-intensity interval training (HIIT) able to prevent the increase of the inflammatory proteins in the testis of spontaneously hypertensive rats (SHR)?

Summary answer
HIIT for 8 weeks inhibits the increase of the tumor necrosis factor alpha (TNFα) and the interleukin 6 (IL6) in the testis of SHR.

What is known already
Hypertension increases the inflammatory cytokines of the cardiovascular system, causing damage in the microcirculation and in the testes. Hypertension is a cause of low fertility in men and exercises are indicated to decrease blood pressure and improve overall health. HIIT is characterized by short periods of exercise, with an intensity equal to or greater than the anaerobic threshold, separated by recovery periods. HIIT can be indicated for cardiac patients, as this type of training improves the cardiac autonomic nervous system and lipemic control in both hypertensive and normotensive individuals.

Study design, size, duration
Male Wistar-Kyoto rats without hypertension and SHRs were divided into 3 groups (n = 4): WKY (Wistar-Kyoto); SHR; and SHR-HIIT (SHRs that performed HIIT). The HIIT was realized in treadmill for 5 days/week for 8 weeks. HIIT started with 5 times of 4 minutes of 100% of the maximum exhaust speed, with active rest intervals of 3 minutes at 60%. There was an increase of once every week until reaching 7 times each session.

Participants/materials, setting, methods
Samples from testicles were used for immunostainig IL6 and TNFα. After the blocking, the sections were subjected to reaction of specific antibodies IL6 (1:50, E–4, sc–28343) and TNFα (1:50, 52B83, sc–257) at 4ºC overnight, and with secondary antibodies m-IgGK (1:200, IgG-HRP, sc–516) at room temperature for 2 hours. Diaminobenzidine (1:50) was used against Harris stained with Hematoxylin and evaluated in the photomicroscope. Data were analyzed by One-Way ANOVA followed by Tukey post test (P &lt; 0.05).

Main results and the role of chance
The immunostainig of IL6 was higher in SHR (10.23 ± 0.47) than in SHR-HIIT (8.69 ± .44) group (P = 0,0237). From the same perspective, immunoexpression of TNFα was higher in SHR (10.10 ± 0.42) than WKY (8.24 ± 0.24) and SHR-HIIT (7.82 ± 0.39) groups (P = 0.0018).

Limitations, reasons for caution
As a limitation of the study, we have no measurement of fertility parameters to affirm that the HIIT improve the fertility because of the reduction of inflammatory mediators.
Wider implications of the findings: The hypertension drugs have a negative effect on fertility. HIIT can be suggested as a treatment for hypertension as an alternative to medication, since HIIT can inhibit the increase of the inflammatory mediators in testis and its consequences to the reproduction. Financial support by São Paulo Research foundation, FAPESP (2018/22682–0).

Trial registration number
2018/22682–0