bluuebunny/crossref_metadata_2025_split huggingface dataset:
Genetic Manipulation of FBP1 Expression in Breast Cancer Cells Reveals the Potential of FBP1 to Disrupt The Warburg Effect, and Nutrient Mediated Adaptive Post-Translational Regulation of FBP1 Protein Levels.
Authors: Ghanem Ali , Troung Boilinh , Wölfl Stefan 
Published: November 2021

Abstract
The key gluconeogenic enzyme Fructose 1,6 bisphosphatase has emerged as a potential metabolic tumour suppressor in recent years. Ablation of FBP1 expression in various tumours fosters metabolic rewiring towards an increased reliance on aerobic glycolysis. While FBP1 is completely repressed in many cancers, breast tumours display distinct FBP1 expression based on their subtype, hence making breast cancer a suitable model for discerning the effects of FBP1 in contexts of cancer initiation and progression. We used ectopic over-expression and CRISPR-Cas9 disruption to establish cell lineages with stable alterations in their FBP1 levels. Complete knock-outs of FBP1 on gene level confirm the major impact of FBP1 on cancer cell metabolism and proliferation. Moreover, our results reveal cancer subtype-specific peculiarities in response to ectopic FBP1 expression including a clear demonstration of an adaptive proteasomal degradation of FBP1 in breast cancer cells, which is regulated by glucose and nutrient availability. In addition to validating the suggested tumour suppressor role of FBP1, our findings demonstrate an efficient adaptive complex regulation of FBP1 expression and its impact on the adaptation of cancer cells to nutrient conditions. Our results highlight the importance of FBP1 ablation on the protein level and its potential relevance to cancer and beyond.
Gas Phase Metallicities of Local Ultra-Luminous Infrared Galaxies Follow Normal Star-Forming Galaxies
Authors: Cooray Asantha , Chartab Nima , Ma Jingzhe , Nayyeri Hooshang , Zillot Preston , Lopez Jonathan , Fadda Dario , Herrera-Camus Rodrigo , Malkan Mathew , rigopoulou Dimitra , Sheth Kartik , Wardlow Julie 
Published: November 2021

Abstract
Despite advances in observational data, theoretical models, and computational
techniques to simulate key physical processes in the formation and evolution of
galaxies, the stellar mass assembly of galaxies still remains an unsolved problem
today. Optical spectroscopic measurements appears to show that the gas-phase
metallicities of local ultra-luminous infrared galaxies (ULIRGs) are significantly
lower than those of normal star-forming galaxies1–3. This difference has resulted
in the claim that ULIRGs are fueled by metal-poor gas accretion from
the outskirts4. Here we report on a new set of gas-phase metallicity measurements
making use of the far-infrared spectral lines of [Oiii]52 μm, [Oiii]88 μm,
and [Niii]57 μm instead of the usual optical lines. Photoionization models have
resulted in a metallicity diagnostic based on these three lines that break the electron
density degeneracy and reduces the scatter of the correlation significantly5.
Using new data from SOFIA and archival data from Herschel Space Observatory,
we find that local ULIRGs lie on the mass-metallicity relation of star-forming
galaxies and have metallicities comparable to other galaxies with similar stellar
masses and star formation rates. The lack of a departure suggests that ULIRGs
follow the same mass assembly mechanism as luminous star-forming galaxies and
∼ 0.3 dex under-abundance in metallicities derived from optical lines is a result
of heavily obscured metal-rich gas which has a negligible effect when using the
FIR line diagnostics.
Association between Blood Lead Levels and Markers of Calcium Homeostasis: A Systematic Review and Meta-Analysis
Authors: Upadhyay Kuldip , Viramgami Ankit , Bagepally Bhavani Shankara , Balachandar Rakesh 
Published: November 2021

Abstract
Introduction:Chronic Pb exposure associated systemic illness are partly posited to involve calcium homeostasis. Present systematic review aims to comprehensively evaluate the association between chronic lead exposure and markers of calcium homeostasis. Methods:Observational studies documenting the changes in calcium homeostasis markers (i.e. serum calcium, parathyroid hormone, vitamin D & calcitonin) between occupationally Pb exposed group and control group were systematically searched from digital databases since inception to March 17, 2021. PRISMA guidelines were adhered during execution. Mean differences of calcium homeostasis markers between the groups were analysed using random-effects model. Heterogeneity was assessed by I2 statistic, while the risk of bias was performed using Newcastle Ottawa Scale. Sub-group, sensitivity and meta-regression analyses were performed where data permitted. Results:Eleven studies were (837 Pb exposed vs 739 controls) identified. Pb exposed group exhibited higher mean 36.13 (95%CI 25.88-46.38) µg/dl blood lead level significantly lower serum calcium, and trend of higher parathyroid levels and lower vitamin D levels than controls. Heterogeneity was high among the studies. Conclusion:Considering the cardinal role of calcium in multiple biological functions, present observations emphasis the need for periodic evaluation of calcium levels and its markers among those with known cumulative exposure to Pb.
Efficacy and Safety of Immunotherapy Compared With Chemotherapy in The Second-Line Treatment of Advanced Esophageal Cancer: A Meta-Analysis and System Review
Authors: Zhu Kang-hao , Xu Cong-cong , Chen Dong , Jin Zi-xian , Ren Si-jia , Shen Jian-fei , Chen Bao-fu 
Published: November 2021

Abstract
Background: Currently, surgical resection, radiotherapy, and chemotherapy are most often utilized in clinical practice, however, they are associated with limited survival benefits. Immunotherapy is currently widely used as the second-line treatment of advanced esophageal cancer, but its effectiveness and safety compared to chemotherapy for advanced esophageal cancer have yet to be proven. Methods: Original articles describing the safety and effectiveness of immunotherapy for advanced esophageal cancer published before February 2021 were retrieved from PubMed, Embase and Cochrane libraries. Hazard ratio (HR) and 95% confidence interval (CI) were calculated and heterogeneity analysis was performed. Results: A total of four studies were included, involving 2087 patients with esophageal cancer. They were included in the meta-analysis and compared immunotherapy and chemotherapy in the treatment of esophageal cancer. Overall survival, progression-free survival, treatment-related adverse events, and objective remission rate were used to evaluate the safety and effectiveness of immunotherapy. Our analysis showed that compared with chemotherapy, immunotherapy gave a significant survival advantage in patients with esophageal cancer ((HR 0.79, 95% CI 0.71-0.88), and fewer treatment-related adverse events at grade 3 or above. Among them, immunotherapy showed high safety (HR 0.38, 95% CI 0.28-0.51), but there was no significant difference in progression-free survival between the two groups (HR1.01, 95%CI 0.79-1.30), The objective response rate showed that immunotherapy has an advantage over chemotherapy (HR 1.67, 95% CI 0.91-3.16). Conclusion: For patients with advanced esophageal cancer, immunotherapy is more effective and safer than chemotherapy as the second-line treatment.
Intergenerational Transmission of Body Mass Index and Associations with Educational Attainment
Authors: Alrouh Hekmat , Bergen Elsje , Zeeuw Eveline , Dolan Conor , Boomsma Dorret I. 
Published: November 2021

Abstract
Background

Individual differences in educational attainment (EA) and physical health, as indexed by body mass index (BMI), are correlated within persons and across generations. The present aim was to assess these associations while controlling for parental transmission.
Methods

We analyzed BMI and EA obtained for 8,866 families from the Netherlands. Data were available for 19,132 persons, including 6,901 parents (mean age 54) and 12,234 of their adult offspring (mean age 32). We employed structural equation modeling to simultaneously model the direct and indirect transmission of BMI and EA from parents to offspring, spousal correlations, and the residual within-person BMI-EA association and tested for gender differences in the transmission parameters.
Results

We found significant intergeneration transmission of BMI and EA from parents to their adult offspring, and substantial spousal correlations (0.23 for BMI and 0.51 for EA). Cross-trait parent to offspring transmission was weak. The strength of transmission was largely independent of parent or offspring gender. About 60% of the EA-BMI correlation in the offspring persisted after taking into account the intergeneration transmission.
Conclusions

The intergenerational transmission for BMI and EA is mainly predictive within traits. Significant spousal and within person correlations in the parental generation are responsible for the effect of parental EA on offspring BMI. Offspring EA and BMI are further correlated beyond parental influences.
Intrinsic Disorder in Proteins Associated With Oxidative Stress-Induced JNK Signaling
Authors: Gehi Bhuvaneshwari R. , Gadhave Kundlik , Uversky Vladimir N. , Giri Rajanish 
Published: November 2021

Abstract
The c-Jun N-terminal kinase (JNK) signaling cascade is a mitogen-activated protein kinase (MAPK) signaling pathway that can be activated in response to a wide range of environmental stimuli. Based on the type, degree, and duration of the stimulus, the JNK signaling cascade dictates the fate of the cell by influencing gene expression through its substrate transcription factors. Oxidative stress is a result of a disturbance in the pro-oxidant/anti-oxidant homeostasis of the cell and is associated with a large number of diseases, such as neurodegenerative disorders, cancer, diabetes, cardiovascular diseases, and disorders of the immune system, where it activates the JNK signaling pathway. Among different biological roles ascribed to the intrinsically disordered proteins (IDPs) and hybrid proteins containing ordered domains and intrinsically disordered protein regions (IDPRs) are signaling hub functions, as intrinsic disorder allows proteins to undertake multiple interactions, each with a different consequence. In order to ensure precise signaling, cellular abundance of IDPs is highly regulated, and mutations or changes in abundance of IDPs/IDPRs are often associated with disease. In this study, we have used a combination of six disorder predictors to evaluate the presence of intrinsic disorder in proteins associated with the oxidative stress-induced JNK signaling cascade, and as per our findings, none of the 18 proteins involved in this pathway are ordered. Highest level of intrinsic disorder was observed in the scaffold proteins, JIP1, JIP2, JIP3; dual specificity phosphatases, MKP5, MKP7; a phospho-serine binding protein 14-3-3ζ, and transcription factor c-Jun. The MAP3Ks, MAP2Ks, MAPKs, TRAFs, and thioredoxin were the proteins that were predicted to be moderately disordered. Furthermore, to characterize the predicted IDPs/IDPRs in the proteins of the JNK signaling cascade, we identified the molecular recognition features (MoRFs), posttranslational modification (PTM), sites and short linear motifs (SLiMs) associated with the disordered regions. These findings will serve as a foundation for experimental characterization of disordered regions in these proteins, which represents a crucial step for better understanding of the roles of IDPRs in diseases associated with this important pathway.
Genetic Characterization of Feline Calicivirus From China Reveals That Amino Acid Mutations at 441-443 in The ORF2 E Region Are Related to Pathogenicity
Authors: Liu Xuejiao , Tao Weijie , Yu Yongle , Yang Haiyan , Shan Hu , Zhang Chuanmei 
Published: November 2021

Abstract
We studied the diversity and regularity of FCV variations in two new feline calicivirus (FCV) isolates QD-7 and QD-164. We compared the genomes of these new strains with 39 from the NCBI database including isolates from China, United States, Germany, South Korea, the United Kingdom and Japan. The nucleotide sequence identities ranged from 75-88 % indicating a high degree of variability. These variations were not related to distributions of the virus by time of isolation and geographical location. Cats that were experimentally infected with the new isolate QD-164 showed typical clinical symptoms of sneezing, fever and conjunctivitis and all recovered within 30 days. In contrast, QD-7 infections were asymptomatic and the virus was cleared within 16 days. These results indicate that QD-7 and QD-164 were naturally attenuated strains. NNS mutations that are characteristic of highly virulent strains at positions 441-443 were absent from QD-7 while QD-164 possessed an N at position 442. This indicated that mutations in regions 441-443 may be linked to disease severity.
Air Processed Cs2AgBiBr6 Lead-free Double Perovskite High-mobility Thin Film Field-effect Transistors
Authors: Abiram Gnanasampanthan , Gourji Fatemeh Heidari , Pitchaiya Selvakumar , Ravirajan Punniamoorthy , Murugathas Thanihaichelvan , Velauthapillai Dhayalan 
Published: November 2021

Abstract
This study focuses on the fabrication and characterization of Cs2AgBiBr6 double perovskite thin film for field-effect transistor (FET) applications. The Cs2AgBiBr6 thin films were fabricated using a solution process technique and the observed XRD patterns demonstrate no diffraction peaks of secondary phases, which confirms the phase-pure crystalline nature. The average grain sizes of the spin-deposited film were also calculated by analysing the statistics of grain size in SEM image and was found to be around 412 (±44) nm the larger grain size was also confirmed by the XRD measurements. FETs with different channel lengths of Cs2AgBiBr6 thin films were fabricated on an electrode deposited heavily doped p-type Si substrate with a 300 nm thermally grown SiO2 dielectric under ideal conditions in air processing under ambient pressure and temperature. The Cs2AgBiBr6 FETs showed a p-type nature with a positive threshold voltage. The on current, threshold voltage and hole-mobility of the FETs decreased with increasing channel length. A high average hole mobility of 0.29 cm2s-1V-1 was obtained for the FETs with a channel length of 30 µm, and the hole mobility was reduced by an order of magnitude (0.012 cm2s-1V-1) when the channel length was doubled. The on current and hole-mobility of Cs2AgBiBr6 FETs followed a power fit, which confirmed the dominance of channel length in electrostatic gating in Cs2AgBiBr6 FETs. A very high-hole mobility observed in or FET that could be attributed to the much larger grain size of Cs2AgBiBr6 film made in this work.
Construction of an Individualized Clinical Prognostic Index Based on Ubiquitination-Associated lncRNA in Clear Cell Renal Cell Carcinoma Patients
Authors: Liu Kun , Liu Xuzhong , Sun Qing , Tang Zhiwang , Wang Gongcheng , Xu Zongyuan 
Published: November 2021

Abstract
Background: The purpose of this study was to identify the ubiquitination-associated genes and co-expressed lncRNAs on the prognosis of clear cell renal cell carcinoma (ccRCC) patients.Methods and patients: We downloaded 530 cases and the corresponding transcriptome profiling from The Cancer Genome Atlas (TCGA) database. We distinguished mRNA and lncRNA expression data from the transcriptome profiling, then extracted the expression of mRNAs that regulate protein ubiquitination. We obtained lncRNAs associated with protein ubiquitination regulation from the lncRNA data by Gene Co-expression Analysis. Cox Regression Analysis of survival time, survival status, and lncRNA expression level was carried out and a Prognostic Index (PI) was constructed.Results: The PI was established based on 8 prognostic lncRNAs that regulate protein ubiquitination, and distinguish the high-risk group patients from all patients. Multivariate analysis indicated that this PI was an individualized clinical prognostic factor for patients with ccRCC. Regarding clinical characteristics, a Ubiquitination-associated Clinical-Prognostic Index (UCPI), containing 8 ubiquitination-related lncRNAs and age, was established and validated with high prognostic efficacy.Conclusion: We established a UCPI containing 8 lncRNAs related to protein ubiquitination. This UCPI may become an appropriate model to predict the prognosis in ccRCC patients and guide clinicians to adjust the follow-up regimen.
Structural Magnetic Resonance Imaging Findings and Histopathological Correlations in Human and Animal Motor Neuron Diseases – A Systematic Review and Meta-Analysis
Authors: Zejlon Charlotte , Nakhostin Dominik , Winklhofer Sebastian , Pangalu Athina , Lewandowski Sebastian , Finnsson Johannes , Piehl Fredrik , Ingre Caroline , Granberg Tobias , Ineichen Benjamin Victor 
Published: November 2021

Abstract
Background: The lack of systematic evidence on neuroimaging findings in motor neuron diseases (MND) such as amyotrophic lateral sclerosis (ALS) hampers the diagnostic utility of magnetic resonance imaging (MRI) in the clinical setting. Moreover, it is unknown how well MND animal models mimic actual human neuroimaging features.Objective: To perform a systematic review and meta-analysis of MRI features in MND and corresponding animal models including their histopathological correlation.Methods: In a comprehensive literature search in Medline, Scopus, and Embase, out of 5941 unique publications, 223 records assessing brain and spinal cord MRI findings in MND were eligible for a qualitative synthesis. Of these, 21 records were included in a random effect model meta-analysis. For the animal systematic review, out of 175 unique publications, 33 records assessed neuroimaging findings in MND animal models. Data mining techniques were exploited for risk of bias assessment.Results: T2-hyperintensities along the corticospinal tract as well as motor cortex T2*-hypointensities, also termed the “motor band sign”, were among the most commonly assessed imaging biomarkers. Our meta-analysis shows that both T2-hyperintensities along the corticospinal tracts and the motor band sign more commonly occur in ALS patients compared to controls (Odds ratio 2.21 [95%-CI: 1.40-3.49] and 10.85 [95%-CI: 3.74-31.44], respectively). Evidence from a limited number of studies suggest these two imaging findings to correlate to focal axonal degeneration/myelin pallor or glial iron deposition on histopathology. Whole brain or regional central nervous system (CNS) atrophy was also frequently assessed. Clinical MND phenotypes such as ALS versus ALS-frontotemporal dementia seem to present with distinct CNS atrophy patterns. Furthermore, certain MRI imaging findings in MND animal models such as brain volume loss show partial resemblance with corresponding human imaging features.Conclusions: Multiple corroborating studies support the notion that MND in humans can be distinguished based on certain MRI features, which also correlate to neuropathological findings. In addition, there is also some overlap with findings in animal MND models. Collectively, this study provides high-grade evidence for the usefulness of MRI in the diagnostic workup of suspected MND cases. Further studies are needed to address the value of MRI for a more exact definition of MND subtypes and prognosticating the disease course.Trial registration number: CRD42020182682
Z-Map Based Cutting Force Prediction for Elliptical Ultrasonic Vibration-Assisted Milling Process
Authors: Li Zhongqun , Xiao Jiandong Xiao , HAN Xiong , ZHANG Weifeng 
Published: November 2021

Abstract
Elliptical ultrasonic vibration-assisted milling (EUVAM) adds high-frequency vibration to conventional milling (CM) to realize high-frequency intermittent milling. It has broad application prospects in the processing of difficult-to-cut materials such as titanium alloys, superalloys and hard and brittle materials. To reveal the mechanism of the highly intermittent cutting nature in EUVAM, according to the motion relationship between cutting edge and workpiece and the Z-map model of the workpiece, a method and its algorithm for calculating undeformed cutting thickness and thus the cutting force in EUVAM are proposed. The simulation results show that EUVAM can improve the actual cutting speed when compared with CM, and the proportion of idle cutting time will directly determine the intermittent degree of the milling process. The experiment of EUVAM is performed to verify the correctness of the proposed cutting force model, and the impact of spindle speed on the cutting force in EUVAM is also analyzed.
A fast radio burst source at a complex magnetised site in a barred galaxy
Authors: Lee K.J. , Xu Heng , Niu J.R. , Chen P. , Zhu Weiwei , Dong Subo , Zhang Bing , Jiang J.C. , Wang B.J. , Xu J.W. , Zhang C.F. , Fu H. , Filippenko Alexei , Peng Eric , Zhou D.J. , Zhang Yongkun , Wang P. , Feng Yi , Li Ye , Brink Thomas , Li Dongzi , Lu Wenbin , Yang Yuan-Pei , Caballero R. N. , Cai C. , Chen M.Z. , Dai Z.G. , Djorgovski S , Esamdin A , Gan H.Q. , Guhathakurta Puragra , Han J.L. , Hao L.F. , Huang Y.X , Jiang P. , Li C.K. , Li Di , Li H. , Li Xin , Li Z.X. , Liu Z.Y. , Luo Rui , Men Y.P. , Niu C.H. , Peng W.X , Qian Lei , Song Liming , Stern Daniel , Stockton A. , Sun Jinghai , Wang Fayin , Wang M. , Wang Na , Wang Weiyang , Wu Xue-Feng , Xiao S. , Xiong Shaolin , Xu Y.H. , Xu Renxin , Yang J. , Yang X , Yao R. , Yi Q.B. , Yue Youling , Yu D.J. , Yu Wenfei , Yuan Jianping , Zhang Binbin , Zhang S.B. , Zhang Shuang-Nan , Zhao Y , Zheng Weikang , Zhu Yan , Zou Jin-Hang 
Published: November 2021

Abstract
Fast radio bursts (FRBs) are highly dispersed radio bursts prevailing in the
universe. The recent detection of FRB~200428 from a Galactic magnetar suggested
that at least some FRBs originate from magnetars, but it is unclear whether the
majority of cosmological FRBs, especially the actively repeating ones, are
produced from the magnetar channel. Here we report the detection of 1863
polarised bursts from the repeating source FRB~20201124A during a dedicated
radio observational campaign of Five-hundred-meter Aperture Spherical radio
Telescope (FAST). The large sample of radio bursts detected in 88 hr over 54
days indicate a significant, irregular, short-time variation of the Faraday
rotation measure (RM) of the source during the first 36 days, followed by a
constant RM during the later 18 days. Significant circular polarisation up to
75\% was observed in a good fraction of bursts. Evidence suggests that some
low-level circular polarisation originates from the conversion from linear
polarisation during the propagation of the radio waves, but an intrinsic
radiation mechanism is required to produce the higher degree of circular
polarisation. All of these features provide evidence for a more complicated,
dynamically evolving, magnetised immediate environment around this FRB source.
Its host galaxy was previously known. Our optical observations reveal that it is
a Milky-Way-sized, metal-rich, barred-spiral galaxy at redshift
z=0.09795+-0.00003, with the FRB residing in a low stellar density, interarm
region at an intermediate galactocentric distance, an environment not directly
expected for a young magnetar formed during an extreme explosion of a massive
star.