bluuebunny/crossref_metadata_2025_split huggingface dataset:
Transcriptome Analysis of The Whole Body Provides Novel Insights Into The Molecular Mechanisms of Growth, Immunity, and Metabolism During The Molting Cycle of The Juvenile Mud Crab Scylla Paramamosain
Authors: Liu Lei , Liu Xiao , Fu Yuanyuan , Fang Wei , Wang Chunlin 
Published: January 2021

Abstract
Background: Molting is an inherent cyclic process that occurs in all crustaceans and is essential for growth, metamorphosis, and reproduction. Recent studies have shown that many organs and systems are involved in molting regulation, including the hepatopancreas, the immune system, and production of ecdysteroids by Y-organs. However, the molecular mechanisms underlying the dynamic process of crab molting remain poorly understood at the whole-individual level. Therefore, we investigated global expression changes in the transcriptomes of the mud crab Scylla paramamosain, the most cultured crab species worldwide. We revealed a cascade of sequential expression events of molting-related genes involved in various aspects of the molting process by using whole-body sequencing of juvenile crabs. Results: RNA-sequencing (RNA-seq) produced 139.49 Gb of clean reads and 20,436 differentially expressed genes (DEGs) among different molting stages. The expression patterns for genes involved in several molecular events critical for molting, such as cuticle reconstruction, cytoskeletal structure remodeling, hormone regulation, immune responses, and metabolism, were characterized and considered as mechanisms underlying molting in S. paramamosain. Among these genes, we identified 10,695 DEGs in adjacent molting stages. In further GO and KEGG analyses, the significantly enriched pathways included structural constituents of cuticle, binding and chitin metabolic processes, steroid hormone biosynthesis, insulin resistance, and amino sugar metabolic processes. The expression profiles of 12 functional genes detected via RNA-Seq were corroborated via real-time RT-PCR assays. The results indicated gene expression profiles across the molting cycle and identified possible activation pathways for future investigation of the underlying molecular mechanisms. Conclusions: This study establishes a comprehensive transcriptomic repertoire for the molting process in S. paramamosain. The data generated in this study will serve as an important transcriptomic resource for the crab research community to facilitate gene and genome annotation and provide fundamental support for future research on molecular mechanisms in this species. Furthermore, the comprehensive analysis results will improve our understanding of morphological variation in the molting cycle and serve as a potential blueprint for future research on molting in crustaceans and other animals.
Identification of acute spinal cord injury and autophagy-related potential key genes，pathways, and targeting drugs through bioinformatics analysis
Authors: Xu Wei-long , Zhao Yan 
Published: January 2021

Abstract
Background: Acute spinal cord injury (ASCI) is considered a form of severe central nervous system damage. At present, research in the fields of spinal surgery and neurology has highlighted the complex mechanisms underlying ASCI, among which autophagy is considered to play a crucial role.Objectives: We aimed to identify the genes and molecular pathways associated with ASCI and autophagy using computational tools and publicly available data, and to identify drugs targeting the relevant genes associated with ASCI and autophagy.Materials and Methods: We used text mining to detect the ASCI and autophagy-associated genes, and the intersection of the two gene sets was selected for gene ontology analysis using the DAVID program. We then constructed protein–protein interaction networks, followed by a functional enrichment analysis, from which we obtained two significant gene modules. Finally, the final list of genes was queried against the Drug Gene Interaction database to find drug candidates targeting the relevant ASCI and autophagy genes.Results: Our analysis identified 156 genes common to both the “ASCI” and “Autophagy” text mining concepts. Gene enrichment analysis yielded two significant gene modules (20 genes), which represent six significant signal pathways and could be targeted by 28 Food and Drug Administration (FDA)-approved drug molecules, and identified the drug–gene interactions. Conclusion: In conclusion, we presented a method to explore the potential key genes, molecular pathways and candidate drugs associated with ASCI and autophagy. As a result, in this method, we identified a total of 20 potential genes, six significant pathways and 28 candidate drugs, which could provides a basis for new trials and the development of novel targeted therapies as potential treatments for ASCI.
Simplified Chinese Version of the Spinal Instability Neoplastic Score in Evaluating Patients with Spinal Metastatic Tumor: A Cross-Cultural Adaptation and Validation
Authors: Chen Jiajia , Yin Mengchen , Li Ningdao , Wang Xiangyu , Xu Guanhua , Bao Guofeng , Xu Chongqing , Ye Jie , Ma Junming , Mo Wen , Wang Dan , Cui Zhiming 
Published: January 2021

Abstract
Background: The Spinal Instability Neoplastic Score (SINS) in simplified Chinese has not been developed so far. This study aimed to translate the SINS into simplified Chinese, adapt it cross-culturally and to validate its psychometric properties in measuring spinal instability in patients with spinal metastatic tumors in the Chinese mainland. Methods: The original version of SINS (in English) was translated and cross-culturally adapted into simplified Chinese according to the internationally recognized guidelines. Internal consistency was evaluated with Cronbach’s alpha. Test-retest reliability was examined among the patients with a 4-week interval. The validity of the Chinese version of SINS (SC-SINS) was assessed by examining its relationship with Kostuik classification. In addition, floor and ceiling effects were considered present if more than 15% of respondents achieved the lowest or highest possible total score. Principal component analysis was conducted to confirm the factor structure of each subscale.Results: No major problems occurred in the forward and back translations of SINS. The internal consistency of SC-SINS was excellent (Cronbach’s a =0.857, ranging from 0.68 to 0.85). Test-retest reliability was also excellent with a value of 0.89, ranging from 0.86 to 0.95. Validity analyses indicated that the SC-SINS was positively and significantly correlated with Kostuik classification. All items showed principal component coefficients of over 0.4. No floor or ceiling effects was found in the SC-SINS.Conclusion: The results indicate that the SC-SINS is reliable and valid in measuring the spinal stability in patients with spinal metastatic tumor.
Long-term Outcome of a Patient With Transcobalamin Deficiency Caused by the Homozygous c.1115_1116delCA Mutation in TCN2 Gene: a Case Report
Authors: Martino Francesco , Magenta Alessandra , Troccoli Maria Letizia , Martino Eliana , Torromeo Concetta , Putotto Carolina , Barillà Francesco 
Published: January 2021

Abstract
Background: Transcobalamin deficiency is a rare autosomal recessive inborn error of cobalamin transport (prevalence: <1/1000000) which clinically manifests in early infancy.Case presentation: We describe the case of a 31 years old woman who at the age of 30 days presented with the classical clinical and laboratory signs of an inborn error of vitamin B12 metabolism. Family history revealed a sister who died at the age of 3 months with a similar clinical syndrome and with pancytopenia. She was started on empirical intramuscular (IM) cobalamin supplements (injections of hydroxocobalamin 1 mg/day for 1 week and then 1 mg twice a week) and several transfusions of washed and concentrated red blood cells. With these treatments a clear improvement in symptoms was observed, with the disappearance of vomiting, diarrhea and normalization of the full blood count. At 8 years of age injections were stopped for about two and a half months causing the appearance of pancytopenia. IM hydroxocobalamin was then restarted sine die. The definitive diagnosis could only be established at 29 years of age when a genetic evaluation revealed the homozygous c.1115_1116delCA mutation of TCN2 gene (p.Q373GfsX38).Currently she is healthy and she is taking 1 mg of IM hydroxocobalamin once a week.Conclusions: Our case report highlights that early detection of TC deficiency and early initiation of aggressive IM treatment is likely associated with disease control and an overall favorable outcome.
Atopic Dermatitis: a Case Report of a 3-year-old Food Allergy Child Sensitive to Multiple Allergens
Authors: Wang Meiyi , Liang Xueqing , Sun Baoqing , Huang Huimin 
Published: January 2021

Abstract
Background: It was estimated that about 10% of the population suffer from food allergy. Atopic dermatitis is often associated with food allergy. In this case report, we presented a child with atopic dermatitis who was sensitive to multiple food and inhaled allergens.Case report: A 3-year-old boy was admitted to the First Affiliated Hospital of Guangzhou Medical University due to skin allergy caused by drinking semi-hydrolyzed infant formula milk powder. Allergen test results showed that the boy was sensitive to multiple food and inhaled allergens. we conducted the follow-up interview by phone to completed the questionnaire.Conclusion: Atopic dermatitis children with multiple food allergies must pay attention to diet adjustment to prevent or delay the development of the disease.
A Rapid Assessment of the Existence (or Survival) of Macro Fauna in Different Land Uses in Kween District of Mount Elgon
Authors: Aggrey Siya , Kato Arthur Martin , Egeru Anthony , Luswata Kizza Charles 
Published: January 2021

Abstract
Objective We explored the effect of different land uses on soil macro fauna species richness, abundance and diversity in Mount Elgon areas of Kween District, Uganda. ResultsSpecies richness was higher on the subsurface of the different land uses than belowground. A total of 647 individuals of both subsurface and belowground macro fauna were identified from the different land uses. Macro fauna abundance significantly varied across the five different land uses (H=10.1, d.f.=4, p=0.04). The average diversity of the subsurface was higher (0.71) than that belowground (0.20). The highest diversity of macro fauna was found in the intercrop of maize-beans followed by natural vegetation. Maize monocrop had the least abundance and diversity of both surface crawling and belowground macro fauna. This could be due the diversity of food and living environments as mixed cropping introduces landscape heterogeneity that accommodates diverse organisms across different land use systems. This study recommends extended studies on taxonomy, ecology and management of soil macro fauna with detailed inventories on the functional groups across land use/cover types and evaluations of varied agricultural intensification regimes on abundance and diversity of soil above and below ground macro fauna.
Adipose-derived Mesenchymal Stem Cells Improve the Healing of Tracheoesophageal Fistula in a Beagle Model&nbsp;
Authors: Wang Yuchao , Yang Shifang , Chen Pingping , Xu Hanyi , Cui Jinghua , Li Jing 
Published: January 2021

Abstract
Background Tracheoesophageal fistula (TEF) is still a devastating clinical problem with high mortality. New clinical strategies were developed to sustain survival time. Mesenchymal stem cells were applied in many clinical wound healing fields. This study aims to investigate the main effect of stem cell to TEF.Material and Method We established a beagle model with TEF by punching the trachea and esophagus membrane and suturing. The beagles were divided into three groups (group 1 = 1, group 2 = 6, group 3 = 6). Group 2 and 3 received a TEF building operation. Group 3 were injected 2 ml stem cells (106 per animal), and group 2 injected saline water with same volume. Group 1 did not receive any intervention. All animals receipted total parental nutrition. The closure degree of fistula tissue was observed by bronchoscope and post-mortem after 35 d.Result Morphologic and histopathologic changes of fistulas were assessed by gross and endoscopic observation. The fistulas diameter was measured. In 35 d postoperatively, group 2 showed that 3 animals died for acute smother, 2 animal died for severe chronic pneumonia and 1 animal with consistent fistula. Group 3 showed that 2 animals fully closed, 3 animals fistula diameter significantly decreased, and 1 animal died for acute smother. In autopsy result, group 2 animals showed severer pneumonia degree than group 3 animals in 35 d.Conclusion The transplantation of stem cells can promote healing degree of TEF without any complications and relieve pneumonia at the same time.
Aberrant FGF15/FGFR4 Signaling Worsens Nonalcoholic Steatohepatitis Severity in FGF21KO Mice
Authors: Yu Youxi , Shi Xiaoju , Zheng Qianqian , Wang Xingtong , Liu Xingkai , Tan Min , Lv Guoyue , Zhang Ping , Martin Robert C. , Li Yan 
Published: January 2021

Abstract
BackgroundPharmacological application of the endocrine fibroblast growth factor15/19 (FGF15/19) and FGF21 holds great promise to be effective therapeutic agents for treating nonalcoholic steatohepatitis (NASH), which is the most severe form of non-alcoholic fatty liver disease (NAFLD) and accepted as a potential precursor of hepatocellular carcinoma (HCC). In our previous studies, we found that FGF21 played a key role in preventing the development of NASH, however, the FGF15/19 mediated-FGFR4 signaling worsened NASH and even contributed to the NASH-HCC transition. MethodsNASH models were established in FGF21KO mice fed with high fat methionine-choline deficient (HFMCD) diet to investigate FGF15/FGFR4 signaling during NASH development. We sought to determine whether FGF15/FGFR4 signaling could alleviate or aggravate NASH in the FGF21KO mice challenged with HFMCD. ResultsSignificant increase of FGF15 production was found in the liver of the NASH-FGF21KO mice, however the increased FGF15 protein was unable to alleviate hepatic lipid accumulation. In contrast, the up-regulated hepatic FGFR4–β‑klotho was found during NASH progression, as evident by hepatocyte injury/repair, fibrosis and potential malignant events, in the NASH-FGF21KO mice. ConclusionThe increased FGF15 production in NASH-FGF21KO mice could not substitute for FGF21 to compensate its lipid metabolic benefits thereby to prevent NASH development. The up-regulated FGFR4/β-klotho was coupled to cellular and molecular events which might associate to carcinogenic transformation.
Asymmetry in Paraspinal Muscles as a Predictor of the Development of Pregnancy-Related Low Back and Pelvic Pain: A Prospective Study.
Authors: Long Gong , Haoning Ma , Ping Yi , Mingsheng Tan 
Published: January 2021

Abstract
Background: The etiology of pregnancy-associated lumbopelvic pain (LPP) is not clear at present. This condition is highly heterogeneous with different development and subtypes, including low back pain (LBP) and pelvic girdle pain (PGP). Paraspinal muscles are of importance to maintain the spine's functional stability, and their weakness participates in the occurrence of LPP. Therefore, this study aims to determine the asymmetry in the paraspinal muscle before pregnancy and evaluating its association with pregnancy-associated LPP.Methods: This was a prospective case-control study conducted from January 2017 and December 2018. 171 subjects (mean age ± SD, 27.4±5.8 years) were finally divided into the LBP group, PGP group, and no LPP group. The mean value of F-CSA's ratio to T-CSA (F/T CSA) was used to determine whether the bilateral paraspinal muscle was asymmetrical. Total muscle CSA (T-CSA), functional CSA (F-CSA), and F/T CSA were measured unilaterally and compared between groups. Logistic regression was performed to determine the risk factors for pregnancy-associated LPP. The Pearson correlation coefficient was performed to test the relationship between asymmetry in F/T-CSA and pain rating.Results: 124 subjects (72.5%) (28.5±5.2 years) had the LPP during the pregnancy. 48 (38.7%) individuals had LBP, and 76 (61.3%) had PGP. A total of 39 (31.5%) women unrecovered from LPP. F/T-CSA was significantly decreased in LBP than those in the PGP group and control group (0.03±0.02 vs. 0.05±0.03 vs. 0.12±0.05, P <0.001). Meanwhile, significant differences were detected in any two groups (all P <0.001). Asymmetry in the paraspinal muscle (adjusted OR=1.5), LBP (adjusted OR=1.6), LPP in a previous pregnancy (adjusted OR=1.4), sick leave≥90 days (adjusted OR=1.2), and heavy labor (adjusted OR=1.2) were risk factors for the unrecovered LPP during pregnancy.Conclusions: Asymmetrical muscular compositions could lead to abnormal biomechanics for the segmental motions. One-lateral-directed physical training and stretching may help decrease the occurrence and severity of this condition.
Comparison of Clinical Staging and Secondary Staging of Cervical Cancer
Authors: Huang Lisheng , Liu Guobing , Zheng Beihong , Li Guang , Wang Hui 
Published: January 2021

Abstract
ObjectiveTo compare the coincidence rate, lymph node metastasis rate, recurrence or uncontrolled rate between the FIGO clinical staging of cervical cancer and the second postoperative staging.MethodsA retrospective analysis of 999 cervical cancer patients diagnosed and treated in Nanfang Hospital from January 2003 to December 2016 to compare two staging methods through SPSS. Results: There is a significant statistical difference between the results of two stages (P＜0.01). Meanwhile the consistency is poor (Kappa value = 0.248 ). There is a significant statistical difference between the number of lymphatic metastases in the two stages (P＜0.01). Meanwhile the number of lymphatic metastases in the two stages are related (P＜0.01), but the consistency is poor ( Kappa value = 0.102). Lymph node metastasis rate with different FIGO staging are different (P<0.01). Lymph node metastasis rate with different postoperative secondary staging are different (P<0.01). There is a significant statistical difference in the number of recurrences or uncontrolled between the two stages (P＜0.01). There is a correlation in the recurrences or uncontrolled number between the two stages (P＜0.01), consistent poor (Kappa value = 0.252). Different FIGO stagings have different recurrence or uncontrolled rates (P<0.01), and different postoperative secondary stagings have different recurrence or uncontrolled rates (P<0.01).ConclusionThere are differences in the coincidence rate, lymph node metastasis rate, recurrence or uncontrolled rate between the two stages. The FIGO staging of cervical cancer needs further improvement.
Efficacy&nbsp;of&nbsp;Neoadjuvant&nbsp;Therapy&nbsp;in&nbsp;Improving&nbsp;Long-Term&nbsp;Survival&nbsp;of&nbsp;Patients&nbsp;With&nbsp;Resectable&nbsp;Rectal&nbsp;Cancer: A&nbsp;Meta-Analysis
Authors: Li Xinlong , Li Xiangyuan , Fu Rongrong , Ng Derry Minyao , Yang Tong , Zhang Yu , Zhang Mengting , Shi Yetan , Gu Yixuan , Lv Chenhui , Chen Gang 
Published: January 2021

Abstract
Background: The impact of neoadjuvant therapy on the long-term prognosis of patients with resectable rectal cancer is controversial. This study aimed to explore the effect of neoadjuvant therapy on the long-term prognosis of patients with resectable rectal cancer. Methods: Four major databases (PubMed, Web of Science, Embase, Cochrane library) were searched to find relevant articles published between January 2000 and July 2020. The main outcome indicators were the 5-year overall survival (OS) and disease-free survival (DFS). Results: Compared with upfront surgery, our meta-analysis showed that 5-year OS (HR: 0.84, 95% Cl: 0.78-0.91) and DFS (HR: 0.91, 95% Cl: 0.87-0.95) were prolonged for patients with resectable rectal cancer after receiving neoadjuvant therapy. The results of subgroup analysis suggested that both neoadjuvant short-course radiotherapy (SCRT) and neoadjuvant chemo-radiotherapy (CRT) could improve the 5-year OS and DFS. The 5-year OS and DFS of patients with stage Ⅱ-Ⅲ rectal cancer increased significantly and the improvement of 5-year OS and DFS could also be observed in mid/low rectal cancer.Conclusion: Neoadjuvant therapy could improve the long-term survival of patients with mid/low rectal cancer in stage Ⅱ-Ⅲ. For the treatment, neoadjuvant SCRT and neoadjuvant CRT were recommended.
Health Personnel Improvement in the Implementation of Shariah’s Ethical Code of Conduct in Tangerang Hospital, Indonesia
Authors: Sulistiadi Wahyu , Rahayu Sri , Veruswati Meita , Asyary Al 
Published: January 2021

Abstract

Background: The Shariah hospital aims to provide health services inclusive of the appropriate code of ethics of Sharia, which exceeds the standard of service expected of conservative hospitals. Hospital care depends on the performance of health professionals. The study aimed to assess the relationship between the implementation of the ethical code of Shariah hospitals and the performance of health personnel.Methods: The study is a quantitative analysis that collects cross-sectional data from 119 health personnel respondents in one Shariah hospital in Indonesia. Data analysis was performed using structural equation modeling (SEM).Results: The implementation of the ethical code of Shariah hospitals, whether explicit or implicit, has a significantly positive influence on the performance and well-being of health personnel, which is evident from the t-value of 4.31 (more substantial than the t-value of 1.960). This implementation should run consistently and with the commitment of all parties.Conclusion: Such insight, in turn, can be counted as an input to an approach to health services, particularly in increasing the performance rates, such as hospital. This study is the first to provide new insight into discussion about shariah hospital’s code of conduct by presenting its beneficiary to not only improve health personnels’ performance but also providing the inclusive health service for all religions and cultures.