bluuebunny/crossref_metadata_2025_split huggingface dataset:
Integrative Analysis of CircRNA Expression and Associated ceRNA Network in Graves’ Disease
Authors: Jiang Zhengrong , Huang Linghong , Chen Lijun , Zhou Jingxiong , Bai Xuefeng , Liang Bo , Cai Qinyan , Chen Xiaoyu , Huang Huibin 
Published: February 2021

Abstract
ObjectiveGraves’ disease (GD) is the most common subtype of autoimmune thyroid disease which have the involvement of circular RNAs (circRNAs) in the pathogenesis. However, it is largely unknown about the role of circRNAs in GD. In present study, we performed a comprehensive study of the circRNAs in GD by bioinformatics analyses.MethodsCircRNAs were detected in plasma of 3 newly diagnosed GD patients and 3 healthy controls. We selected 2 up-regulated and 1 down-regulated circRNAs with different expression in GD for validation by transcriptase-quantitative polymerase chain reaction in both GD and healthy control subjects. The GTRD base was used for predicting the transcript factors of the different expression of circRNAs. Then the competing endogenous RNAs(ceRNAs) network was assembled and the analysis of the Gene Ontology and Kyoto Encyclopedia of Genes and Genomes were performed to predict the functions of different expression of circRNAs.ResultsA total of 366 different expression of circRNAs were detected in GD. The exonic circRNA hsa_circ_0090364 was validated as an up-regulated molecule in GD. 82 transcript factors in promoter region for hsa_circ_0090364 were predicted. The ceRNAs network revealed that the miR-378 family members acted as the target microRNAs of hsa_circ_0090364 and its downstream genes, and also unveiled the involvement of hsa_circ_0090364 in pathogenesis of GD.ConclusionsHsa_circ_0090364 might play a role of ceRNA through sponging up miR-378 family members, which demonstrated that circRNAs had an effect on regulation in GD.
Salinization Depresses Soil Enzyme Activity in Metal-polluted Soils Through Increases in Metal Toxicity
Authors: Fayez Raiesi , Azadi Nahid 
Published: February 2021

Abstract
Salinity may increase metal mobilization and toxicity with a potentially significant consequence for soil enzymatic activity and nutrient cycling. The goal of this study was to investigate changes in soil enzyme activity in response to salinization of a clay loam soil artificially polluted with cadmium (Cd) and lead (Pb) during an incubation experiment. Soil samples were polluted with Cd, Pb, and a combination of Cd and Pb, pre-incubated for aging, and then salinized with three levels of NaCl solution, and were finally incubated for 120 days. NaCl salinity consistently increased the mobilization of Cd and Pb with greater increases at high than low salinity levels. While the increased Cd mobilization was greater in co-polluted than Cd-polluted soils, the increase of Pb mobilization was lower in co-polluted than Pb-polluted soils at high salinity level. The salinity-induced increases in metal mobilization and toxicity significantly depressed soil microbial respiration, microbial biomass content and enzymatic activities. The increased soil electrical conductivity, Cd mobilization and pH after salinization were the most important factors governing microbial activity and biomass in metal-polluted soils. Changes in microbial biomass and mobile metal pool with increasing salinity had the major effects on enzyme activities, particularly under the combined metals. Secondary salinization of metal polluted soils would impose an additional toxicity stress on enzymatic activities as biochemical indicators of soil quality, and therefore should be avoided for the maintenance of soil microbial and biochemical functions, especially in arid regions. In metal-polluted soils, the observed responses of enzymes to salinity can be used to advance our knowledge of microbial processes when modelling the carbon and nutrient cycling.
Wear and Frictional Behavior of Al 7075/FA/SiC Hybrid MMC’s using Response Surface Methodology
Authors: Mandava Ravi Kumar , Reddy Vajram Venkata , Rao Veeravalli Rama Koteswara 
Published: February 2021

Abstract
In the present research work an effort has been made to study the wear and frictional behavior of Aluminium Metal Matrix composite (Al 7075 as a base alloy and fly ash (FA) and silicon carbide (SiC) as reinforcements) by using the stir casting method. To carry out this work, the wt. % of reinforcements FA (2.5%, 5%, 7.5% and 10%) and SiC (2.5%, 5%, 7.5% and 10%)have been used 5%, 10%, 15% and 20%. Initially, the mechanical studies have been conducted and the best mechanical properties obtained at 20 wt. % of FA and SiC. Later on, the composite was fabricated by 20 wt. % of FA, SiC reinforcements are used to check the wear and frictional behavior on a pin-on-disc machine at the dry condition. The dry sliding wear behavior was carried out at various input parameters such as applied force (10N, 20N, and 30N), sliding velocity (1.5m/s, 3m/s, and 4.5m/s), and sliding distance (1000 m, 2000 m, and 3000 m). Further, a scanning electron microscope (SEM) are used to observe the mixing of reinforcements and examine the worn surfaces. A response surface methodology (RSM) is the reasonable and accurate method for conducting the experiments and identifying the optimal wear parameters. Moreover, the RSM was helped to identify the most significant factor, which was the influence on the wear rate. Finally, it is decided that the applying force is the utmost significant factor that leaves an effect on wear rate. The sliding velocity and distance are acting as the lesser influence on the performance indicator.
Petroleum Hydrocarbon Concentrations in the Surface Sediments of Two Major Sea Ports, Bay of Bengal, India
Authors: Arumugam Sundaramanickam , Balachandar Kumar , Saravanan Kumaresan , Idayachandiran Gopalsamy , Nithin Ajith 
Published: February 2021

Abstract
This investigation was made to assess the seasonal accumulation pattern of total petroleum hydrocarbon (TPH) in sediments along the two major ports such as Ennore and Tuticorin, located in Southeast coast of India. The level of TPH was found to vary from 2.05µg g− 1to 17.47 µg g− 1. To establish the relationship among the TPH with total organic carbon (TOC) and sediment components, the techniques of correlation analysis and principal component analysis (PCA) were used. The higher accumulation of TPH during monsoon season indicates that all wastes discharged on land was washed and got deposited on the Ennore coast. The significant positive correlation with TOC was noticed and the evidences indicated that the sedimentary organic carbon is acting as a strong binding agent for TPH.
Evaluation of the Role of Staphylococci in the Pathomechanism of Conjunctivitis
Authors: Jasińska Ewa , Bogut Agnieszka , Magryś Agnieszka , Olender Alina 
Published: February 2021

Abstract
Purpose: Determination of the association between ica genes and phenotypic biofilm formation in staphylococcal isolates involved in conjunctivitis, their antibiotic resistance as well as detection of selected virulence characteristics: adhesion to epithelial cells and in vitro cytotoxicity.Methods: The study included 26 Staphylococcus aureus (SA) and 26 Staphylococcus epidermidis (SE) isolates. The presence of icaAD genes and ica operon was determined by the PCR assay. Phenotypic biofilm formation was verified using the microtiter plate assay. Antibiotic resistance was performed using the disc diffusion method. Staphylococcal ability to attach to host cells was assessed by flow cytometry. Cytotoxicity on epithelial cells was evaluated by LDH assay.Results: The ica genes were detected in 26.9% of SE and in 42.3% of SA isolates. Only 15.3% of isolates (SE) were positive for both the icaAD and the ica operon. Phenotypically, 19.2% of SE isolates were strong biofilm producers, among which three were both icaAD- and ica operon-positive. 26.9% of SA isolates were strong biofilm producers. Methicillin resistance (MR) was detected in 34.6% of SE and 26.9% of SA isolates. 75% of MR isolates were multidrug resistant. SA isolates adhered to host cells more extensively than SE. SA isolates released higher level of LDH than SE.Conclusions: Adherence abilities were commonly observed in staphylococci associated with conjunctivitis. However, low prevalence of isolates positive for a complete and functional ica locus and low prevalence of strong biofilm producers was detected. SA adhered to a greater extent to eukaryotic cells than SE and were more cytotoxic.
Psychometrics of Persian Version of the 11 items De Jong Gierveld Loneliness Scale among an Iranian Older Adults Population during COVID-19 Pandemic
Authors: Hosseini Lida , Nia Hamid Sharif , Froelicher Erika Sivarajan , Farahani Mansoureh A. 
Published: February 2021

Abstract
Background: Due to the urgent emphasis on quarantine during the COVID-19 pandemic, the possibility of feelings of loneliness, especially in older adults, became an important nursing priority. Therefore, is important to identify loneliness quickly and respond appropriately to prevent, reduce or treat it. In order to do so, the aim of this study was to translate and evaluated the Persian version of De Jong Gierveld Loneliness Scale for older adults. Method: The original scale was translated into Persian using the World Health Organization protocol of forward-backward translation technique. Face validity and content validity; followed by EFA and CFA. The sample was 400 olddr adults including who were recruited via online data gathering. Lastly, reliability was assessed through the Average Inter-Item Correlation (AIC), Cronbach’s alpha and McDonald’s omega.Results: The results showed that Persian version of Loneliness Scale had two factors namely social loneliness (5 items) and emotional loneliness (3 items) and the combined score explained 45.66% of the total variance of this scale. In addition, all goodness of fit indices confirmed a two factors model fit and all of the reliability indices were excellent.Conclusion: The Persian version of the Loneliness Scale is useful and suitable for detecting social loneliness and emotional loneliness in older adults during the COVID-19 pandemic.
Cold-water Coral Microbiome and Environmental Microbial Communities in a Remote NE Atlantic Submarine Canyon Setting: Microbial Diversity, Coral Health and Prospects
Authors: Appah J. K. M. , Dillane E. , Lim A. , ’Riordan R. O , O’Reilly L. , Macedo L. , Wheeler A. J. 
Published: February 2021

Abstract
In the Porcupine Bank Canyon, Lophelia pertusa and Madrepora oculata are the main framework-forming corals producing three dimensional structures which provide a home for a range of benthic fauna and microbial communities. To understand the roles and functions that microbes perform in coral health in the Porcupine Bank Canyon, three groups of samples (corals, sediment and water) were collected between 600–800 m depth. DNA was extracted from these samples and metabarcoding was performed on the V3-V4 region of the 16S RNA gene using Illumina technology. The coral microbiome showed greater microbial diversity than both the surrounding sediment and water communities. The genera Pseudomonas, Pseudoalteramonas and Photobacterium were the bacterial communities conserved at 100% coverage of coral samples whereas at the order-level classification Clostridiales, Bacteroidales, Flavobacteriales, Rhodobacterales and Rickettsiales were in high abundance in all the coral samples. A disproportionate distribution of probiotic and pathogenic bacterial groups at the different levels of classification was observed on the corals. Corals do not appear, at present, to be stressed by climate induced changing environmental conditions in the upper Porcupine Bank Canyon. Overall, the corals in the Porcupine Bank Canyon are in a healthy state despite the detection of pathogenic bacterial groups. However, the current trend of climate change and subsequent deep-sea warming could shift the bacterial composition towards a more dominant pathogenic bacterial community, with serious implications for coral health and stability of this important ecosystem.
Machine Learning Analysis of Volatolomic Profiles in Breath Can Identify Non-invasive Biomarkers of Liver Disease
Authors: Thomas Jonathan , Roopkumar Joanna , Patel Tushar 
Published: February 2021

Abstract
Disease-related effects on hepatic metabolism can alter the composition of chemicals in the circulation and subsequently in breath. The presence of disease related alterations in exhaled volatile organic compounds (VOC) could provide a basis for non-invasive biomarkers of hepatic disease. This study examined the feasibility of combining global VOC (volatolomic) profiles from breath analysis and machine learning to develop signature pattern-based biomarkers for cirrhosis. Breath samples were analyzed using thermal desorption-gas chromatography-field asymmetric ion mobility spectroscopy to generate volatolomic profiles. Samples were collected from 35 persons with cirrhosis, 4 with non-cirrhotic portal hypertension, and 11 healthy participants. Molecular features of interest were identified to determine their ability to classify cirrhosis or portal hypertension. A molecular feature score was derived that increased with the stage of cirrhosis and had an AUC of 0.78 for detection. Chromatographic breath profiles were utilized to generate machine learning-based classifiers. Algorithmic models could discriminate presence or stage of cirrhosis with a sensitivity of 88-92% and specificity of 75%. These results demonstrate the feasibility of volatolomic profiling to classify clinical phenotypes without identifying specific compounds. These studies will pave the way in developing non-invasive biomarkers of liver disease based on volatolomic signatures found in breath.
Design and Experimental Study of Pressure Compensation System for Full-Ocean-Depth Gas-Tight Sediment Sampler
Authors: Liu GuangPing , Jin Yongping , Peng You-duo , Wan Bu-yan 
Published: February 2021

Abstract
Aiming at the requirement of the full-ocean-depth (operating water depth 11000 meters) manned submersible to carry out the gas-tight sampling operation of the abyss seabed sediment, a kind of full-ocean-depth carrier submersible mechanical hand-held, full-ocean-depth gas-tight sediment sampler (GTSS) with the function of pressure-retaining and coring is designed. Firstly, the volume change model of pressure compensator is established, and it is pointed out that the volume of pressure compensator is about 16.14% equal to the volume of gas-tight sediment sampler (GTSS). Secondly, the pressure compensator is analyzed and calculated, and the relationship between the precharge pressure of the pressure compensator, the nominal volume of the pressure compensator and the pressure holding effect of the gas-tight sediment sampler (GTSS) is studied. The results show that with the increase of gas precharge pressure in the pressure compensator, the final pressure of the sampler also increases. Under the same precharge pressure condition, the larger the nominal volume of the pressure compensator, the greater the final pressure of the sampler. Finally, the air tightness test method is designed by using the developed gas tightness sampler of the full-ocean-depth product, and the change of the final pressure in the gas tight sampler under different precharge pressure is observed. The test results are consistent with the simulation results, indicating the correctness of the pressure compensation system (PCS) model.
Distinctive Alteration of Neuropeptide Y Expression Responsible for Neuro-Proliferation Following Zebrafish Spinal Cord Injury
Authors: Cui Chun , Wang Lin-Fang , Huang Shu-Bing , Zhao Peng , Chen Yong-Quan , Wu Yi-Bo , Qiao Chen-Meng , Zhao Wei-Jiang , Shen Yan-Qin 
Published: February 2021

Abstract
In strong contrast to the limited repair within the mammalian central nervous system, the spinal cord of adult zebrafish is capable of regeneration following injury. Understanding the mechanism underlying neural regeneration and functional recovery in spinal cord-injured zebrafish may lead to effective therapies for human spinal cord injury (SCI). Since neuropeptide Y (NPY) plays a protective role in the pathogenesis of several neurological diseases, in the present study, the effects of NPY on neuronal repair and subsequent recovery of motor function in adult zebrafish post-SCI were evaluated. Real-time quantitative PCR (qRT-PCR), in situ hybridization (ISH) and immunostaining of NPY revealed decreased NPY expression at 12 hours (h), 6 days (d) and 21 d after SCI. Double-immunostaining for NPY and Islet-1, a motoneuron marker, showed that NPY was expressed in spinal cord motoneurons. NPY morpholino (MO) treatment resulted in suppressed locomotor recovery and axon regrowth. PCNA and Islet-1 double-staining showed suppressed motoneuron proliferation in NPY-MO zebrafish. Similar to NYP, the mRNA level for NPY1R was also expressed within motoneurons and downregulated at 12 h and 21 d after SCI. Collectively, these data suggest that NPY expression in motoneurons promotes locomotor recovery and axon regrowth in adult zebrafish, possibly by regulating motoneuron proliferation through the activation of NPY1R.
Analysis on The Risk of Respiratory Virus Transmission By Air Conditioning System Operation-Based on Experimental Evidence
Authors: Jia Yonghong , Xiang Yue , Guo Shurui , Guo Lei , Guo Luyao , Cheng Zhu , Zhang Yin , Zhang Li , Long Enshen 
Published: February 2021

Abstract
It is important to know whether SARS-CoV-2 is spread through the air-conditioning systems. Taking the central air-conditioning system as an example, we analyze the mechanism and potential health risk of respiratory virus transmission in air-conditioned rooms, and propose a method to study the risk of virus transmission in central air-conditioning systems by investigating the data from medical experiments. The virus carrying capacity and the decay characteristics of indoor pathogen droplets are studied in this research. Additionally, the effects of air temperature and relative humidity on the virus survival in the air or on surfaces are investigated. The removal efficiency of infectious droplet nuclei by air-conditioning filter was then determined. Thus, the transmission risk during the operation of centralized air-conditioning system is evaluated. The results show that the indoor temperature and humidity are controlled in the range of 20-25℃ and 40%-70% by central air-conditioning during the epidemic period, which not only benefits the health and comfort of residents, but also weakens the vitality of the virus. The larger the droplet size, the longer the viruses survive. Since the filter efficiency of air-conditioning filter increases with the increase of particle size, increasing the number of air changes of the circulating air volume can accelerate the removal of potential pathogen particles. Therefore, scientific operation of centralized air conditioning systems during the epidemic period has more advantages than disadvantages.
One Pot Synthesis, Quantum Chemical Stimulation and Anticancer Evaluation of Some New 8-Azacoumarin Derivatives
Authors: Rizk Sameh , Megahed Mohamed , Badawy Monda , Aly Mohamed 
Published: February 2021

Abstract
New anticancer agents are highly needed to overcome cancer cell resistance. Synthesis of newly pyrazole, derivatives via heterocyclic ring opening of azacoumarin promoted with grinding and ultrasonic reaction conditions. Efficient solvent less one pot synthesis can be well progressed to afford the good yield of new heterocyclic products that were characterized by IR, 1H-NMR, MS and micro-analytical data. Anticancer evaluation for the synthesized compounds exhibited good cytotoxiciy. The anti-liver cancer activity of all compounds was screened in vitro against hepatocellular carcinoma (HCC) cell lines (HepG-2) by viability assay. The synthesized compounds were evaluated for their anticancer activity and found to exhibit promising activities. All new compounds were tested for possible anti-cancer activity against HepG-2 cell lines in comparison to the reference drug doxorubicin (DOX). Compound 8 was the most active against the liver carcinoma cell line (HepG-2) giving promising half-maximal inhibitory concentration (IC50) value of 27.5 ± 1.3 μg/mL, compared with DOX with IC50 value of 0.36 ± 0.02 μg/mL. However it has weak cytotoxic effects against normal rat hepatocytes with 50% cytotoxic concentration (CC50) = 1820.5 µg/ml (= > 500 µg/ml). Compound 8 was selected to be tested in combination with ionizing gamma radiation. Gene expression levels of the cell cycle inhibitor p21 and caspase-3 was quantified. As well as, Oxidative stress was quantified by measuring the concentration of malondialdehyde (MDA), and antioxidant activity of reduced gluthatione (GSH). This study concluded that the new derivative of the azacomarin compound has an effective anti-cancer effect and it was found that using the new compound with ionizing radiation at a dose of 8 Gy improves the effectiveness of the compound on liver cancer cells.
A Novel Collaboration-Oriented Requirement Model Mapping and Classification Method Based on Domain Knowledge for Complex Mechatronic Products
Authors: Yue Jiguang , Wu Fuchao , Liu Jun , Wang Ping , Cui Zhaxin , Zou Hongyu 
Published: February 2021

Abstract
Complex mechatronic products include inter-disciplinary, inter-field, and inter-departmental requirement information. Aiming at the low digitization rate of existing product requirement information, a structured and digital product requirement model (RM) is defined. Besides, a collaboration-oriented requirement model mapping and classification method for complex mechatronic products is proposed, which realizes the automatic generation of the domain requirement models and the mapping between the requirement models and knowledge graph, thus improving the digitization rate of the product in the requirement phase. A simulated military plane horizontal tail control system is used as a case study to prove the effectiveness and feasibility of this method. Based on Hoteam PLM8.0 platform, a management architecture and the display path of product requirement model are given, which can provide a reference for the research and development of product lifecycle model management (PLMM) system.