bluuebunny/crossref_metadata_2025_split huggingface dataset:
A 3-D virtual human thermoregulatory model to predict whole-body and organ-specific heat-stress responses
Authors: Unnikrishnan Ginu , Hatwar Rajeev , Hornby Samantha , Laxminarayan Srinivas , Gulati Tushar , Belval Luke N. , Giersch Gabrielle E. W. , Kazman Josh B. , Casa Douglas J. , Reifman Jaques 
Published: June 2021

Abstract
Objective
This study aimed at assessing the risks associated with human exposure to heat-stress conditions by predicting organ- and tissue-level heat-stress responses under different exertional activities, environmental conditions, and clothing.

Methods
In this study, we developed an anatomically detailed three-dimensional thermoregulatory finite element model of a 50th percentile U.S. male, to predict the spatiotemporal temperature distribution throughout the body. The model accounts for the major heat transfer and thermoregulatory mechanisms, and circadian-rhythm effects. We validated our model by comparing its temperature predictions of various organs (brain, liver, stomach, bladder, and esophagus), and muscles (vastus medialis and triceps brachii) under normal resting conditions (errors between 0.0 and 0.5 °C), and of rectum under different heat-stress conditions (errors between 0.1 and 0.3 °C), with experimental measurements from multiple studies.

Results
Our simulations showed that the rise in the rectal temperature was primarily driven by the activity level (~ 94%) and, to a much lesser extent, environmental conditions or clothing considered in our study. The peak temperature in the heart, liver, and kidney were consistently higher than in the rectum (by ~ 0.6 °C), and the entire heart and liver recorded higher temperatures than in the rectum, indicating that these organs may be more susceptible to heat injury.

Conclusion
Our model can help assess the impact of exertional and environmental heat stressors at the organ level and, in the future, evaluate the efficacy of different whole-body or localized cooling strategies in preserving organ integrity.
Physical activity accumulation along the intensity spectrum differs between children and adults
Authors: Rantalainen Timo , Ridgers Nicola D. , Gao Ying , Belavý Daniel L. , Haapala Eero A. , Finni Taija 
Published: June 2021

Abstract
Purpose
Detailed exploration of physical activity accumulation with fine grading along the intensity spectrum has indicated the potential pragmatic utility of such an approach. However, it is currently unclear what sorts of accumulation patterns along particular intensity bands are found in the children and adult populations. Therefore, we conducted a comparison of activity accumulation in specific intensity bands between four distinct populations: children, adults with sedentary lifestyles, habitual joggers, habitual marathon runners.

Methods
Free-living waist-worn accelerometry records from 28 children aged 7 to 11, and 61 adults aged 25 to 35 were analysed. Activity intensity was evaluated in 5 s non-overlapping epochs as mean amplitude deviation (MAD) and normalised to acceleration intensities corresponding to walking at 3 metabolic equivalents of a task (METs). Adult data were normalised to 0.091 g MAD based on literature, and data from children to 0.170 g MAD based on laboratory experimentation. The normalised epoch values were divided into 100 intensity gradations.

Results
Children accumulated more activity in 0.74 to 1.58 normalised acceleration intensities (all p < 0.005) compared to adults. Adult joggers/runners accumulated more activity in normalised acceleration intensities from 7.1 to 11.1 compared to the other groups (p < 0.008).

Conclusion
The primary bulk of children’s free-living activities are of relatively low intensity not likely to provoke cardiometabolic improvement. These sorts of explorations could be used in informing intervention development aiming at optimising healthy development. Evidence is mounting to justify randomised controlled trials based on intervention targets identified based on exploring the intensity spectrum.
Locked fracture dislocations of the proximal humerus: postoperative results and a proposed modification of the classification
Authors: Schmalzl Jonas , Graf Annika , Gilbert Fabian , Kimmeyer Michael , Gerhardt Christian , Lehmann Lars-Johannes 
Published: June 2021

Abstract
Background
Locked dislocations of the glenohumeral joint are disabling and often painful conditions and the treatment is challenging. This study evaluates the functional outcome and the different prosthetic treatment options for chronic locked dislocations of the glenohumeral joint and a subclassification is proposed.

Methods
In this single-center retrospective case series, all patients with a chronic locked dislocation treated surgically during a four-year period were analyzed. Constant score (CS), Quick Disabilities of Shoulder and Hand Score (DASH), patient satisfaction (subjective shoulder value (SSV)), revision rate and glenoid notching were analyzed.

Results
26 patients presented a chronic locked dislocation of the glenohumeral joint. 16 patients (62%) with a mean age of 75 [61–83] years were available for follow-up at 24 ± 18 months. CS improved significantly from 10 ± 6 points to 58 ± 21 points (p < 0.0001). At the final follow-up, the mean DASH was 27 ± 23 and the mean SSV was 58 ± 23 points. The complication rate was 19% and the revision rate was 6%; implant survival was 94%. Scapular notching occurred in 2 (13%) cases (all grade 1).

Conclusion
With good preoperative planning and by using the adequate surgical technique, good clinical short-term results with a low revision rate can be achieved. The authors suggest extending the Boileau classification for fracture sequelae type 2 and recommend using a modified classification to facilitate the choice of treatment as the suggested classification system includes locked posterior and anterior dislocations with and without glenoid bone loss.

Level of evidence:
IV.
Alarin alleviated cardiac fibrosis via attenuating oxidative stress in heart failure rats
Authors: Li Jinshuang , Ding Hao , Li Yong , Zhou Hao , Wang Wanhong , Mei Yong , Zhang Ronglin 
Published: June 2021

AbstractThe present study was to explore whether alarin could alleviate heart failure (HF) and attenuate cardia fibrosis via inhibiting oxidative stress. The fibrosis of cardiac fibroblasts (CFs) was induced by angiotensin (Ang) II. HF models were induced by ligation of the left anterior descending artery to cause ischemia myocardial infarction (MI) in Sprague–Dawley rats. Alarin (1.0 nM/kg/d) was administrated by intraperitoneal injection for 28 days. The decreases of left ventricular (LV) ejection fraction (EF), fractional shortening (FS), the maximum of the first differentiation of LV pressure (LV ± dp/dtmax) and LV systolic pressure (LVSP), and the increases of LV volume in systole (LVVS), LV volume in diastole (LVVD), LV end-systolic diameter (LVESD) and LV end-diastolic diameter (LVEDD) in MI rats were improved by alarin treatment. The increases in the expression levels of collagen I, collagen III, and transforming growth factor (TGF)-β were inhibited by alarin treatment in CFs and in the hearts of MI rats. The levels of NADPH oxidase (Nox) activity, superoxide anions and malondialdehyde (MDA) levels were increased, and the level of superoxide dismutase (SOD) activity was reduced in Ang II-treated CFs, which were reversed by alarin. Nox1 overexpression reversed the effects of alarin on attenuating the increases of collagen I, collagen III and TGF-β expression levels induced by Ang II in CFs. These results indicated that alarin improved HF and cardiac fibrosis via inhibiting oxidative stress in HF rats. Nox1 played important roles in the regulation of alarin effects on attenuating CFs fibrosis induced by Ang II.
Home activities and cognitive skills in relation to early literacy and numeracy: testing a multifactorial model in preschoolers
Authors: Bonifacci Paola , Trambagioli Nicole , Bernabini Luca , Tobia Valentina 
Published: June 2021

AbstractThe aim of the present study was to test environmental and cognitive variables as possible cross-domain predictors of early literacy and numeracy skills. One hundred forty-eight preschool children (mean age = 64.36 months ± 3.33) were enrolled in the study. The battery included a home literacy and home numeracy questionnaire, measures and phonological and visuo-spatial working memory, tasks tapping response inhibition, and predictors of literacy (vocabulary, phonological awareness, letter knowledge) and numeracy (magnitude comparison, number knowledge) skills. The structural equation model indicated that verbal working memory and, to a lesser extent, inhibition represented cross-domain predictors, whereas home numeracy activities and visuo-spatial working memory explained additional variance only for early numeracy skills. Implications for parents and educators are discussed.
Test–retest assessment of non-contrast MRI sequences to characterise and quantify the small bowel wall in healthy participants
Authors: Alyami Ali. S. , Williams Hannah. G. , Argyriou Konstantinos , Gunn David , Wilkinson-Smith Victoria , White Jonathan. R. , Alyami Jaber , Gowland Penny. A. , Moran Gordon. W. , Hoad Caroline. L. 
Published: June 2021

Abstract
Objective
Quantitative Magnetic Resonance Imaging sequences have been investigated as objective imaging biomarkers of fibrosis and inflammation in Crohn’s disease.

Aim
To determine the repeatability and inter- and intra-observer agreement of these measures in the prepared small bowel wall.

Methods
Ten healthy participants were scanned at 3 T on 2 separate occasions using T1 and T2 relaxometry, IVIM-DWI and MT sequences. Test–retest repeatability was assessed using the coefficient of variation (CoV) and intra-class correlation coefficients (ICCs) were used to evaluate the intra- and inter-observer agreement

Results
Test–retest repeatability in the bowel wall was excellent for apparent diffusion coefficient (ADC), magnetisation transfer ratio (MTR), T1, and diffusion coefficient D (CoV 5%, 7%, 8%, and 10%, respectively), good for perfusion fraction (PF) (CoV 20%) and acceptable for T2 (CoV 21%). Inter-observer agreement was good for the T2, D and ADC (ICC = 0.89, 0.86, 0.76, respectively) and moderate for T1 (ICC = 0.55). Intra-observer agreement was similar to inter-observer agreement.

Discussion
This study showed variable results between the different parameters measured. Test–retest repeatability was at least acceptable for all parameters except pseudo-diffusion coefficient D*. Good inter- and intra-observer agreement was obtained for T2, ADC and D, with these parameters performing best in this technical validation study.
Tackling the scaling-up problem of digital health applications
Authors: Richter Peggy , Harst Lorenz 
Published: June 2021

Abstract
Aim
The purpose of this editorial is to provide guidance for the readers concerning the broad realm of approaches towards successful implementation of digital health applications into the health care system. Recent developments due to the challenges posed by the COVID-19 pandemic are used as a current angle.

Subject and Methods
All contributions within the special issue were scanned for their most decisive contribution to the special issue and the field of implementation science, with a focus on digital health. Micro, meso, and macro layers of implementation processes, as well as the technological perspective itself, are used as broad categories for sorting the contributions and structuring the special issue.

Results
The ten contributions to this special issue cover micro (n = 1), technology (n = 1), meso (n = 4) and macro (n = 2) perspectives on the implementation process of digital health applications. Two further contributions also tackle the issue from a wider perspective when aiming to structure telemedicine application types and barriers encountered when implementing digital health.

Conclusion
Considering the wide array of research fields represented in this special issue, an emphasis is put on the importance of interdisciplinary work required for tackling the scale-up problem of digital health. As such, the special issue can assist in leveraging the full potential of digital health, not only when dealing with situations as out-of-the-ordinary as the current pandemic but also well beyond that, for example when dealing with the upcoming challenges of demographic change.
Metabarcoding of Soil Fungi from Different Urban Greenspaces Around Bournemouth in the UK
Authors: Marczylo Emma L. , Macchiarulo Sameirah , Gant Timothy W. 
Published: June 2021

AbstractSoil microbes are important for public health. Increasing urbanisation is adversely affecting soil microbiota, which may be contributing to the global rise of immune-related diseases. Fungi are key components of urban environments that can be negatively impacted by altered land-use, land-management and climate change, and are implicated in the development and exacerbation of non-communicable diseases such as allergy, asthma and chronic inflammatory conditions. Fungal metagenomics is building knowledge on fungi within different environments (the environmental mycobiome), fungi on and within the human body (the human mycobiome), and their association with disease. Here, we demonstrate the added value of a multi-region metabarcoding approach to analyse soil mycobiomes from five urban greenspaces (lawns, parklands, bareground, young forest and old forest). While results were comparable across the three regions (ITS1, ITS2 and LSU), each identified additional fungal taxa that were unique to the region. Combining the results therefore provided a more comprehensive analysis across all fungal taxonomic ranks, identifying statistically significant differences in the fungal composition of the five soil types. Assignment of fungal taxa into ecological guilds revealed those differences of biological relevance to public health. The greatest differences were between the soil mycobiome of lawns and forests. Of most concern was the significant increase in the known human allergensAlternaria,Bipolaris,CladosporiumandFusariumwithin urban lawn and parklandvsforest soils. By improving our understanding of local variations in fungal taxa across urban greenspaces, we have the potential to boost the health of local residents through improved urban planning.
Incidence of HIV in Sub-Saharan Africa, 2000–2015: The Interplay Between Social Determinants and Behavioral Risk Factors
Authors: Jahagirdar Deepa , Walters Magdalene , Vongpradith Avina , Dai Xiaochen , Novotney Amanda , Kyu Hmwe H. , Wang Haidong 
Published: June 2021

AbstractHIV incidence in sub-Saharan Africa declined substantially between 2000 and 2015. In this analysis, we consider the relative associations of nine structural and individual determinants with this decline. A linear mixed effects model of logged HIV incidence rates versus determinants was used. The data were from mathematical modelling as part of the 2019 Global Burden of Disease Study in 43 sub-Saharan African countries. We used forwards selection to determine a single final model of HIV incidence rate. The association of economic variables and HIV knowledge with incidence was found to be driven by education, while ART coverage had the largest impact on other determinants’ coefficients. In the final model, education years per capita contributed the most to explaining variation in HIV incidence rates; a 1-year increase in mean education years was associated with a 0.39 (− 0.56; − 0.2, t = − 4.48 p < 0.01) % decline in incidence rate while a unit increase in ART coverage was associated with a 0.81 (− 1.34; − 0.28, t = − 3.01, p < 0.01) % decline in incidence rate.
Approximate reasoning with fuzzy rule interpolation: background and recent advances
Authors: Li Fangyi , Shang Changjing , Li Ying , Yang Jing , Shen Qiang 
Published: June 2021

AbstractApproximate reasoning systems facilitate fuzzy inference through activating fuzzy if–then rules in which attribute values are imprecisely described. Fuzzy rule interpolation (FRI) supports such reasoning with sparse rule bases where certain observations may not match any existing fuzzy rules, through manipulation of rules that bear similarity with an unmatched observation. This differs from classical rule-based inference that requires direct pattern matching between observations and the given rules. FRI techniques have been continuously investigated for decades, resulting in various types of approach. Traditionally, it is typically assumed that all antecedent attributes in the rules are of equal significance in deriving the consequents. Recent studies have shown significant interest in developing enhanced FRI mechanisms where the rule antecedent attributes are associated with relative weights, signifying their different importance levels in influencing the generation of the conclusion, thereby improving the interpolation performance. This survey presents a systematic review of both traditional and recently developed FRI methodologies, categorised accordingly into two major groups: FRI with non-weighted rules and FRI with weighted rules. It introduces, and analyses, a range of commonly used representatives chosen from each of the two categories, offering a comprehensive tutorial for this important soft computing approach to rule-based inference. A comparative analysis of different FRI techniques is provided both within each category and between the two, highlighting the main strengths and limitations while applying such FRI mechanisms to different problems. Furthermore, commonly adopted criteria for FRI algorithm evaluation are outlined, and recent developments on weighted FRI methods are presented in a unified pseudo-code form, easing their understanding and facilitating their comparisons.
Increased iron content in the heart of the Fmr1 knockout mouse
Authors: Talvio Karo , Kanninen Katja M. , White Anthony R. , Koistinaho Jari , Castrén Maija L. 
Published: June 2021

AbstractTrace elements have important functions in several processes involved in cellular homeostasis and survival. Dysfunctional metal ion homeostasis can make an important impact on cellular defence mechanisms. We assessed the concentrations of 23 trace minerals in different tissues (brain, spleen, heart and liver) of Fmr1 knockout (KO) mice that display the main phenotype of Fragile X syndrome (FXS), an intellectual disability syndrome and the best-known monogenic model of autism spectrum disorder (ASD). Altogether, seven minerals—Cu, Fe, K, Mg, Mn, Na, and P—were above the detection limit with the analysis revealing increased iron content in the heart of Fmr1 KO mice. In addition, levels of iron were higher in the cerebellum of the transgenic mouse when compared to wild type controls. These results implicate a role for dysregulated iron homeostasis in FXS tissues and suggest that defective iron-related mechanisms contribute to increased tissue vulnerability in FXS.
Activity of trastuzumab emtansine (T-DM1) in 3D cell culture
Authors: Boyer Jean Zheng , Phillips Gail D. Lewis , Nitta Hiro , Garsha Karl , Admire Brittany , Kraft Robert , Dennis Eslie , Vela Elizabeth , Towne Penny 
Published: June 2021

Abstract
Background
Cell spheroids and aggregates generated from three-dimensional (3D) cell culture methods are similar to in vivo tumors in terms of tissue morphology, biology, and gene expression, unlike cells grown in 2D cell cultures. Breast cancer heterogeneity is one of the main drug resistant mechanisms and needs to be overcome in order to increase the efficacy of drug activity in its treatments.

Methods
We performed a unique 3D cell culture and drug efficacy study with trastuzumab emtansine (Kadcyla®, T-DM1) across five breast cancer cell lines (BT-474, SK-BR-3, MDA-MB-361, MDA-MB-175, and MCF-7) that were previously investigated in 2D cell culture. We performed HER2 IHC staining, cell viability experiments, Gene-protein-assay (GPA), and T-DM1 internalization studies.

Results
We obtained significantly different results including higher IC50 for some of the cell lines. Our GPA showed some significant heterogeneous HER2 gene and protein expression in 3D cultured spheroids or aggregates. The fluorescent images also showed that a longer incubation time is needed for T-DM1 to be internalized effectively into 3D cultured spheroids or aggregates.

Conclusion
Our study demonstrated that the difference of T-DM1 drug activity in 3D spheroids or aggregates might be due to tumor heterogeneity and less efficient internalization of T-DM1 that is not seen using 2D cell culture models. Drug studies using 3D cell culture are expected to provide biologically relevant models for determining drug activity in tumor tissue in future drug response and resistance research.
Integrating a Sport-Based Trauma-Sensitive Program in a National Youth-Serving Organization
Authors: Shaikh M. , Bean C. , Bergholz L. , Rojas M. , Ali M. , Forneris T. 
Published: June 2021

AbstractThere is a pressing need to equip youth-serving community organizations to respond to the unique needs of trauma-exposed children. Early prevention measures can be an effective means of redirecting children to self-regulatory healing, while facilitating their transition toward strength-based thriving. Sport can offer a powerful opportunity to reach these children; however there remains little information on how to effectively develop, deliver, evaluate, and sustain trauma-sensitive sport programs in a community context. The purpose of this paper is to outline a case study of integrating sport-based trauma-sensitive practices with BGC Canada’s national Bounce Back League program. An interdisciplinary partnership of academic, community, and practice experts used a community-based participatory action research approach, paired with a knowledge translational approach, to guide the process of program development. Mixed methods (e.g., surveys, logbooks, interviews, focus groups, online communications) were used to generate ongoing insights of staff’s training experiences, successes and challenges of program implementation, and potential impact of program on club members. Several stages of program development are described, including: (a) collaboratively planning the program; (b) piloting the program to three clubs; (c) adapting the program using pilot insights; (d) expanding the adapted program to ten clubs; and (e) creating opportunities to maintain, sustain, and scale-out practices throughout grant duration and beyond. Lessons learned regarding the leadership team’s experiences in terms of developing, adapting, and integrating trauma-sensitive practices in this community context are shared.