bluuebunny/crossref_metadata_2025_split huggingface dataset:
Platelet Rich Fibrin (PRF) along with Coronally Repositioned Flap (CRF) compared with that of CRF alone in the management of isolated Miller class-I gingival recession defects: A non-randomized controlled clinical trial
Authors: Kurien J Tony , RM Baiju , Narayan Vivek 
Published: April 2021

Periodontal plastic surgical techniques for root coverage are aimed to attain optimal results with less patient discomfort. Platelet-rich fibrin (PRF) containing a wide array of growth factors within fibrin network has shown beneficial results in healing of tissues. Study evaluated clinical effects of PRF along with Coronally Repositioned Flap (CRF), test group, and compared it with CRF alone, control group, in the treatment of Miller Class I gingival recession. From the subjects who attended the out-patients clinic, a non-randomized controlled clinical study was done on those who satisfied the inclusion criteria. In the control group 29 patients and in the test group 27 patients completed six months of study period. Clinical parameters pertaining to periodontal health and recession anatomy were recorded. Student t-test was used for intra and inter-group comparison. Multiple linear regression model was developed using three predictor variables. Mean root cover percentage in PRF and CRF groups were 96.25±6.89 and 94.59±10.31 respectively. PRF group showed reduction in gingival thickness at sixth month. Linear regression model showed a negative association of baseline gingival recession value with complete root coverage. CRF+PRF as well as CRF alone are equally effective in attaining &#62;90% root coverage in Miller Class I gingival recession. The observed clinical benefits along with less invasive procedure involved in PRF technique, shows that it is a promising biomaterial in terms of objective appraisal. However, additional studies overcoming the limitations noticed are needed to ascertain the findings.
Optimization of Renewable Energy Production by Utilizing Methane Gas And plastic waste from sources at the Puuwatu landfill Kendari City
Authors: Gunawan Yuspian , Karimuna La , Kano M. Lukas , Endriatno Nanang 
Published: April 2021

This study aims to determine how much electric power can be generated from methane gas obtained from organic waste and how much electrical energy has not been utilized from the potential methane gas produced and how much volume of pyrolysis oil is produced from plastic waste produced by the people of the City. Kendari. To obtain the electrical energy produced, the volume of waste is first calculated so that the potential for waste (Q), Biogas Production Volume (VBS), and Methane Gas Volume (VGM) can be obtained. Referring to Kadir's research, 2012, the volume of pyrolysis oil produced by Kendari City is obtained. The results showed that the energy produced from the Puuwatu TPA in a daily average was 288,466,5332 kWh. While the energy flowing through the Mandirin Energi area is 125 houses and each house with a power of 450 VA (MCB 2 Ampere), the daily count used to fulfill the energy independent area is 1,080 kWh, so that energy has not been utilized from its potential. in Puuwatu TPA is 287,386.5332 kWh. While the production of plastic waste in Kendari City per year is 792 tons, the average plastic waste produced in 500 grams is 423.25 ml of pyrolysis oil or the equivalent of 1000 grams produces pyrolysis oil of 845.6 milliliters, so the amount of plastic waste in Kendari City per year year it can produce pyrolysis oil of 670 428 000 liters.

Keywords: Organic Waste, Methane Gas, Electrical Energy, Pyrolysis Oil, Plastric Waste.
The role of cystatin C and various methods of glomerular filtration rate calculation in evaluation of renal dysfunction in children with acute infectious diarrhea
Authors: Chugunova O. L. , Grebеnkina E. Yu. , Usenko D. V. , Volodina I. I. , Galeeva E. V. , Drozhzhin E. A. , Korsunskiy А. А. , Legoshina N. Yu. , Muzyka A. D. , Naryshkina M. I. , Pavlushkina L. V. , Rykova А. S. 
Published: April 2021

Rationale: Acute infectious diarrhea (AID) is the fourth leading cause of death among children < 5 years worldwide. Kidney damage is one of the poorly studied aspects of pediatric AID. The level of serum cystatin C is independent on gender and age, and it is highly informative even in the early stages of renal dysfunction.Aim: To optimize diagnosis of renal dysfunction in children with moderate AID through comparison of different methods of glomerular filtration rate (GFR) calculation and determination of serum cystatin C level.Materials and methods: Observational cross-sectional study enrolled 80 children in pediatric hospital with moderate AID not followed by hemolytic uremic syndrome. Serum creatinine and serum cystatin C levels were determined in all the patients in acute period with GFR calculating according to Schwartz equation in unmodified (1976) and modified (2009) versions. GFR was also calculated using a single-factor equation based on serum cystatin C level.Results: GFR in acute period, calculated according to unmodified and to modified Schwartz equation was in patients < 3 years (n = 40) 115.47 ± 3.33 ml/min/1.73 m2and 98.56 ± 2.84 ml/min/1.73 m2(p < 0.001), in patients 3-7 years 132.13 ± 4.2 ml/min/1.73 m2and 108.85 ± 3.84 ml/min/1.73 m2(p < 0.001), respectively. Increased serum cystatin C level (> 950 ng/ml) occurred in 18 patients (22%). In other patients level of serum cystatin C remained within the reference range or lower. The risk of acute kidney injury development based on two indicators - GFR according to the modified Schwartz equation and GFR according to the equation used cystatin C level - occurred in 4 (10%) patients aged 1-3 years and in 1 (2.5%) child in the age group 3-7 years; risk, based on one indicator - the calculation of GFR according to the formula using cystatin C - in 8 (20%) and 9 (22.5%) children, respectively, and based on the assessment of only GFR according to the modified Schwartz equation - in 3 (7.5%) children of both age groups.Conclusion: We have confirmed that the GFR values calculated with usage of unmodified Schwartz equation (1976) are higher than those calculated with usage of modified Schwartz equation (2009) and taking into account the level of cystatin C.Thus, usage of unmodified Schwartz equation for GFR calculation in infants and preschool children seems incorrect. Level of serum cystatin C is promising marker permitted to select patients with risk of acute kidney injury development among children in acute period of moderate AID.
A Preliminary Study Examining the Validity of Brief Trauma Screening Tools for Young Adolescents in School-Based Health Centers
Authors: Soleimanpour Samira , Schapiro Naomi A. , Faxio Adrienne , Brindis Claire D. 
Published: April 2021

Background and PurposeThere are very limited brief, validated, open access screening tools for trauma symptoms in adolescent populations. This study aimed to test two brief tools used with adults in primary care settings for use with adolescents.MethodsYouth (n = 77) completed the Posttraumatic Stress Disorder (PTSD) Checklist (PCLC-2), the Primary Care PTSD Screen for Diagnostic and Statistical Manual of Mental Disorder, Fourth Edition (DSM-IV; PC-PTSD), and the PTSD Reaction Index for DSM-5 (reference tool). Sensitivities and specificities were analyzed.ResultsThe PCLC-2 and PC-PTSD demonstrated high sensitivity and specificity with adolescents when using lower cutoff scores than those recommended for adults.ConclusionsThe PC-PTSD and PCLC-2 have the potential to be used as brief screens with adolescents. Additional research is needed to further examine their validity with larger, diverse youth samples in primary care and school-based settings.
Development and Testing of an Acute Care Nurses' Job Satisfaction Scale (ACNJSS)
Authors: Yasin Yasin M. , Kerr Michael S. , Wong Carol A. , Bélanger Charles H. 
Published: April 2021

Background and PurposeFactors affecting nurses' job satisfaction in the acute care setting may differ from nurses working in other settings. The aim of this study was to develop a new tool that measure the job satisfaction of acute care nurses who provide direct patient care.MethodsContent validity then exploratory factor analysis (EFA) were used for validation of the new tool using a random sample of 349 acute care nurses.FindingsThe EFA resulted in 31 items suggesting 6 factors were present in the instrument. The identified factors were: achievement/job interest/responsibility, hospital policy, quality of supervision, peer support/work condition, growth/advancement, and benefits/job security.ConclusionA new tool for measuring acute care nurses' job satisfaction was developed based on a robust theoretical foundation and demonstrated sound psychometric properties.
Development and Testing of a Method for Detecting Lujo Virus RNA by Reverse Transcription and Real Time Polymerase Chain Reaction
Authors: Naidenova E. V. , Dedkov V. G. , Agafonov D. A. , Senichkina A. M. , Safonova M. V. , Kutyrev V. V. 
Published: April 2021

The aim of the study was to develop and assess the efficacy of a method for Lujo virus RNA detection in clinical and biological samples using one-step real-time RT-PCR.Materials and methods. In order to select the conservative regions of the genome, we utilized the available in GenBank database Lujo virus sequences (https://www.ncbi. nlm.nih.gov/genbank) aligned in BioEdit 7.2.5 software package ( (IbisBiosciences, USA). To conduct one-round RTPCR, reverse transcriptase and TaqF-polimerase were used. Recombinant Escherichia coli strain, XL1-Blue, containing pGEM-T plasmid with inserted synthetically-generated fragment of the virus genome, was produced to make positive control sample (PCS). Constructed recombinant plasmids were used for creating RNA-containing PCS with protective protein shell of MS2-phage. Determination of specificity of the developed method was performed with the help of control panel of RNA and DNA of 23 viral strains related to 10 families; the sensitivity – the panel of biological samples artificially contaminated with PCS. Further testing was carried out at the premises of laboratory of the Russian-Guinean Center for Epidemiology and Prevention of Infectious Diseases (Kindia, Republic of Guinea) on 265 blood sera from practically healthy persons, 110 blood sera of cattle, 83 suspensions of ticks, and 165 suspensions of organs of small mammals collected in the territory of Guinea.Results and discussion. Two conservative polymerase gene fragments have been chosen as targets for Lujo virus RNA detection using RT-PCR. The combination of primers and probes has been experimentally selected, optimum composition of reaction mixture for PCR and mode of RT-PCR set-up established, as well as control samples C+, internal control, positive control sample developed. Sensitivity of the proposed method is 5·103 GE /ml, specificity – 100 %.
Role of <em>Ixodes Pavlovskyi</em> (Acari, Ixodidae) in Borreliosis Epizootic Process at the Island Russky
Authors: Nikitin A. Ya. , Sabitova Yu. V. , Rar V. A. , Morozov I. М. , Gordeiko N. S. , Allenov A. V. , Kaverina G. B. , Babkin I. V. , Tikunova N. V. , Andaev E. I. 
Published: April 2021

The incidence of Ixodes tick-borne borreliosis (ITBB) at the Island Russky (Primorsk Territory) is higher than at the mainland. There is a bidominant structure of the tick population with mass occurrence of Ixodes persulcatus and I. pavlovskyi at the island. Due to the higher infectivity and aggressiveness of I. persulcatus, it is suggested that it plays a key role in high activity of the ITBB focus at the island.The aim of the study: to characterize the role of I. pavlovskyi in the borreliosis epizootic process based on the study of the structure of the borrelia genotype complex isolated from Ixodes ticks at the Island Russky.Materials and methods. The ticks were collected from vegetation at three sites. Ixodide species were determined morphologically and using PCR for mitochondrial gene cox1 and nuclear multicopy locus ITS2. Infection of 233 ticks was analyzed with the species identification confirmed by all methods. Borrelia genospecies were determined by multiplex two-round PCR with the primers specific to the gene fragments 5S and 23S rRNA of Borrelia burgdorferi sensu lato complex.Results and discussion. Coexistence of I. persulcatus and I. pavlovskyi at the island was confirmed, with different ratios in space and a gradual increase in the proportion of the latter species. The circulation of three etiological agents of ITBB: Borrelia garinii (predominantly in I. pavlovskyi), B. bavariensis and B. afzelii (in I. persulcatus) was identified. Thus, an active natural focus of ITBB has formed at the island, probably including two uninsulated chains of borrelia circulation. One chain combines I. persulcatus and small and large mammals as the tick feeders. The second – I. pavlovskyi that can feed on mammals and birds. Due to the spatial variability of the ixodide population structure at different sections of the island, the ratio of the circulating borrelia species can change, and this species becomes the main vector of the pathogenic agents in areas of I. pavlovskyi absolute dominance.
Infection Rate in the Population of Ticks <em>Hyalomma marginatum</em> in the Territory of the Crimean Hemorrhagic Fever (CHF) Natural Focus and Assessment of the Link with the CHF Incidence in the Stavropol Region
Authors: Tsapko N. V. , Dubyansky V. M. , Gazieva A. Yu. , Ashibokov U. M. , Volynkina A. S. 
Published: April 2021

Objective of the study was to assess the infection rate in the population of the main CCHF virus vector Hyalomma marginatum and its impact on the CHF incidence in the Stavropol Region.Materials and methods. The data on spontaneous infection of H. marginatum ticks in the territory of the CHF natural focus in the Stavropol Region for the period 2012–2018 are presented. In total, more than 22.000 H. marginatum specimens pooled in 1546 samples were tested. The indication of CCHF virus was performed by PCR method using the reagent set for detecting RNA of the CCHF virus “AmplySens® CCHFV-FL” (produced by Central Research Institute of Epidemiology, Russia). To determine the individual infection rate in pools Beklemishev`s method was used. The data on CCHF incidence and amount of tick-bitten persons are taken from official statistics and Annual Epidemiological Report on CHF Incidence provided by the Rospotrebnadzor Administration in the Stavropol Region for the period of 2012–2018.Results and discussion. RNA of CCHF virus was detected in 161 (10.4 %) out of 1546 pooled ticks. The highest infection rate (20 %) was among nymphs. The infection rate for pooled males of H. marginatum was 11 % and for females – 8.5 %. Circulation of Crimean-Congo hemorrhagic fever virus is confirmed in major part of the Stavropol Region. The average infection rate among H. marginatum population was 1.54 % for the period of 2012–2018, ranging from 0.23 % in 2014 to 2.97 % in 2017. It is established that the level of infection rate among H. marginatum population does not affect the CHF incidence in the Stavropol Region. Probably it is not the number of infected ticks in the population that determines the CHF incidence rate but their abundance.
Features of the Biological Properties of <em>Vibrio cholerae</em> Isolated during the Monitoring of Water Bodies in Rostov-on-Don from 1989 to 2018
Authors: Ezhova M. I. , Levchenkо D. А. , Arkhangelskaya I. V. , Kruglikov V. D. , Nepomnyashchaya N. B. 
Published: April 2021

The goal is to study the features of the biological properties of Vibrio cholerae strains found in environmental objects (EO) on the territory of Rostov-on-Don from 1989 to 2018.Materials and methods. Bacteriological, molecular biological and statistical methods were used to study 73 strains of cholera vibrios O1, O139 and 1702 strains of nonO1/nonO139 serogroups isolated over a 30-year period.Results and discussion. It was found that in Rostovon-Don, 78.0 % of toxigenic strains were isolated from all isolated from environmental protection in Russia, among which 2 were genetically unchanged. The V. cholerae O1 classical strain and 72 V. cholerae El Tor strains were found, of which 70 % belonged to the Ogawa serovar, and 3 % of the nontoxigenic strains were susceptible to the classical phage. A “very weak direct relationship” was statistically revealed between the isolation of V. cholerae O1, O139 and V. cholerae nonO1/nonO139 strains, in which the dominant serogroups (O67, O76, O75, O53, O16) were identified, and 18 % of the strains showed atypical agglutinability. Revealing the biological characteristics of V. cholerae strains contributes to the optimization of monitoring studies.
Venatorbacter Cucullus Gen. Nov Sp. Nov a Novel Bacterial Predator
Authors: Saeedi Ahmed , Cummings Nicola , McLean Denise , Connerton Ian F. , Connerton Phillippa L. 
Published: April 2021

Abstract
A novel Gram-stain negative, aerobic, halotolerant, motile, rod-shaped, predatory bacterium ASxL5, was isolated from a bovine slurry tank in Nottinghamshire, UK using Campylobacter hyointestinalis as prey. Other Campylobacter species and members of the Enterobacteriaceae were subsequently found to serve as prey. Weak axenic growth on Brain Heart Infusion agar was achieved upon subculture without host cells. The optimal growth conditions were 37°C, at pH 7. Transmission electron microscopy revealed some highly unusual morphological characteristics related to prey availability. Phylogenetic analyses using 16s rRNA gene sequences showed that the isolate was related to members of the Oceanospirillaceae family but could not be classified clearly as a member of any known genus. Whole genome sequencing of ASxL5 confirmed the relationship to members the Oceanospirillaceae. Database searches revealed that several ASxL5 share 16s rRNA gene sequences with several uncultured bacteria from marine, and terrestrial surface and subsurface water. We propose that strain ASxL5 represents a novel species in a new genus. We propose the name Venatorbacter cucullus gen. nov., sp. nov. with ASxL5 as the type strain.
Effect of The FOCUS Program on The Self-Efficacy of Caregivers for Patients With Advanced Lung Cancer
Authors: zhou qinxue , Huang Lihua , Yang Su , Han Hui , Shen Lijuan , Cai Jianli , Xing MeiYuan 
Published: April 2021

Abstract
Objective To investigate the effect of the FOCUS program on the self-efficacy of caregivers of patients with advanced lung cancer.Method In this experimental study, a total of 108 caregivers for patients admitted to a tertiary comprehensive hospital from April 2019 to December 2019 were recruited based on inclusion and exclusion criteria. Based on their admission dates, the caregivers were divided into a control group (54 cases), which only completed routine measures, and a test group (54 cases), which completed the FOCUS Program in addition to routine measures. At the 4th week of the intervention, general self-efficacy, caregiving burden, coping styles and mood states were compared between the control group and the test group.Results The general self-efficacy scale, caregiver burden scale, simple coping style scale and mood state scale were compared between the two groups at the 4th week of the intervention and at 8 weeks after the intervention.After the intervention, the self-efficacy scores of the test group (24.37±4.375) were higher than those of the control group (17.50±3.479), and the difference was statistically significant (t=-9.032, P<0.05); the caregiving burden scores of the test group (41.65± 2.735) were lower than those of the control group (47.33±7.066), and the difference was statistically significant (t=5.514, P<0.05); the coping style scores of the experimental group (33.20±5.533) were significantly higher than those of the control group (28.76±3.420) (t=-5.021, P<0.05); the mood state scale scores of the test group (106.17±12.400) were lower than those of the control group (127.65±11.754), and the difference was statistically significant (t=9.239, P<0.05).The results showed that the intergroup comparisons, group comparisons and interactions were statistically significant (P<0.05).Conclusion The FOCUS Program can effectively improve the self-efficacy of caregivers, reduce their caregiving burden improve their coping styles and enhance their mood states.
Efficacy of INtensive Treatment Versus Standard Treatment of COmpound DanshEn Dripping Pills in Refractory Angina Patients With Incomplete Revascularization (INCODER&nbsp;Study): Study Protocol for a Multicenter, Double-blind, Randomized Controlled, Superiority Trial
Authors: Wu Zexuan , Xu Danping , Wu Zhen , Chen Ailan , Xu Lin , Zhang Yuling , Ling Li , Zhou Yan , Liu Yin , Dong Yugang , Chen Yili 
Published: April 2021

Abstract
Introduction: Patients with incomplete revascularization (ICR) tend to develop refractory angina despite optimal medical therapy. Compound Danshen Dripping Pills (CDDP) is a widely used antianginal drug in China and is showed to significantly alleviate myocardial ischemia. Previous studies showed dose-efficacy tendency when increasing doses of CDDP. The study is aim to investigate the efficacy and safety of intensive doses of CDDP in patients with refractory angina with ICR.Methods and Analysis: The INCODER study is a multicenter, double-blind, randomized controlled, superiority trial. We plan to recruit 250 patients aged 18 to 85 years with a diagnosis of refractory angina with ICR. Patients will be randomized (1:1) to intensive treatment group (CDDP 20 pills three times per day) or control group (10 pills CDDP and 10 pills placebo three times per day). Patients will have a 6-week medication period and be followed up every two weeks. The primary end point is the change of total exercise time from baseline to week 6 as assessed by cardiopulmonary exercise testing (CPET). Secondary end points include changes in frequency of angina, Canadian Cardiovascular Society angina class, nitroglycerin use, Seattle Angina Questionnaire scores, O2 uptake kinetics and other parameters as measured by CPET, and levels of plasma C-reactive protein, homocysteine and N-terminal pro-B-type natriuretic peptide. Safety events related to CDDP use will be monitored.Ethics and dissemination: The research had been approved by the Clinical research and laboratory animal ethics committee of the First Affiliated Hospital, Sun Yat-sen University ([2019]65). The results will be reported through peer-reviewed journals, seminars and conference presentations.Trial registration number: www.chictr.org.cn (ChiCTR2000032384). Registered on 27 April 2020.
Hilbert' s First Problem and the New Progress of Infinity Theory
Authors: Wang Xijia 
Published: April 2021

Abstract
In the 19th century, Cantor created the infinite cardinal number theory based on the "1-1 correspondence" principle. The continuum hypothesis is proposed under this theoretical framework. In 1900, Hilbert made it the first problem in his famous speech on mathematical problems, which shows the importance of this question. We know that the infinitesimal problem triggered the second mathematical crisis in the 17-18th centuries. The Infinity problem is no less important than the infinitesimal problem. In the 21st century, Sergeyev introduced the Grossone method from the principle of "whole is greater than part", and created another ruler for measuring infinite sets. At the same time, the development of the infinity theory provides new ideas for solving Hilbert's first problem, and provides a new mathematical foundation for Cosmic Continuum Theory.