bluuebunny/crossref_metadata_2025_split huggingface dataset:
Dietary fat quality impacts metabolic impairments of type 2 diabetes risk differently in male and female CD-1® mice
Authors: Unger Allison L. , Jetton Thomas L. , Kraft Jana 
Published: October 2021

AbstractMetabolic impairments associated with type 2 diabetes, including insulin resistance and loss of glycaemic control, disproportionately impact the elderly. Lifestyle interventions, such as manipulation of dietary fat quality (i.e. fatty acid (FA) composition), have been shown to favourably modulate metabolic health. Yet, whether or not chronic consumption of beneficial FAs can protect against metabolic derangements and disease risk during ageing is not well defined. We sought to evaluate whether long-term dietary supplementation of fish-, dairy- or echium-derived FAs to the average FA profile in a U.S. American diet may offset metabolic impairments in males and females during ageing. One-month-old CD-1® mice were fed isoenergetic, high-fat (40 %) diets with the fat content composed of either 100 % control fat blend (CO) or 70 % CO with 30 % fish oil, dairy fat or echium oil for 13 months. Every 3 months, parameters of glucose homoeostasis were evaluated via glucose and insulin tolerance tests. Glucose tolerance improved in males consuming a diet supplemented with fish oil or echium oil as ageing progressed, but not in females. Yet, females were more metabolically protected than males regardless of age. Additionally, Spearman correlations were performed between indices of glucose homoeostasis and previously reported measurements of diet-derived FA content in tissues and colonic bacterial composition, which also revealed sex-specific associations. This study provides evidence that long-term dietary fat quality influences risk factors of metabolic diseases during ageing in a sex-dependent manner; thus, sex is a critical factor to be considered in future dietary strategies to mitigate type 2 diabetes risk.
The prevalence of human papillomavirus in paediatric tonsils in Southwestern Ontario
Authors: Wojtera M , Barrett J W , Strychowsky J E , Dzioba A , Paradis J , Husein M , Salvadori M I , Nichols A C 
Published: October 2021

AbstractObjectiveTo determine the prevalence of human papillomavirus in paediatric tonsils in Southwestern Ontario, Canada.Materials and methodsPatients aged 0–18 years undergoing tonsillectomy were recruited. Two specimens (left and right tonsils) were collected from each participant. Tonsillar DNA was analysed using quantitative polymerase chain reaction to determine the presence of human papillomavirus subtypes 6, 11, 16 or 18.ResultsA total of 102 patients, aged 1–18 years (mean age of 5.7 years), were recruited. Ninety-nine surveys were returned. There were 44 females (44.4 per cent) and 55 males (55.6 per cent). Forty patients (40.4 per cent) were firstborn children and 73 (73.7 per cent) were delivered vaginally. Six mothers (6.1 per cent) and one father (1.0 per cent) had prior known human papillomavirus infection, and one mother (1.0 per cent) had a history of cervical cancer. All tonsil specimens were negative for human papillomavirus subtypes 6, 11, 16 and 18.ConclusionNo human papillomavirus subtypes 6, 11, 16 or 18 were found in paediatric tonsil specimens from Southwestern Ontario.
Free-ranging dogs as a potential threat to Iranian mammals
Authors: Nayeri Danial , Mohammadi Alireza , Qashqaei Ali T. , Vanak Abi Tamim , Gompper Matthew E. 
Published: October 2021

AbstractFree-ranging domestic dogs Canis familiaris threaten wildlife species through predation, hybridization, competition for resources, and by contributing to the transmission of pathogens. The impacts of predation may be problematic, but in many regions the interactions of free-ranging dogs and wildlife are poorly studied. To determine the extent of the impacts of attacks by free-ranging dogs on Iranian mammals, we reviewed nearly 2 decades of social and traditional media reports and the scientific literature to gather data from across the country. We identified 160 free-ranging dog attacks (79 from academic articles, 14 from social media, and 67 from a variety of news websites) from 22 of the country's 31 provinces. Attacks by dogs were reported on 17 species, including nine Carnivora, six Artiodactyla, one Rodentia, and one Lagomorpha species. Most of the reported attacks on carnivores were on felids, including the Asiatic cheetah Acinonyx jubatus (n = 19), Eurasian lynx Lynx lynx (n = 18), caracal Caracal caracal (n = 10) and Pallas's cat Otocolobus manul (n = 8). Attacks on Artiodactyla were primarily reported for goitered gazelle Gazella subgutturosa (n = 47). Most of these attacks occurred within or adjacent to protected areas (n = 116, 73%), suggesting that free-ranging dogs are one of the most important human-associated threats to wildlife species even in protected landscapes. The impact of free-ranging dogs may be hampering conservation, and therefore we suggest some practical policy guidance for managing the impacts of free-ranging dogs on threatened species.
Enumeration and instantaneous mobility analysis of a class of 3-UPU parallel manipulators with equilateral triangular platforms
Authors: Boztaş Sercan , Kiper Gökhan 
Published: October 2021

AbstractIn this study, several joint axis orientations on equilateral platforms and the limbs of 3-UPU parallel manipulators (PMs) are examined. The generated joint layouts for the platforms were matched with each other to generate and enumerate manipulator architectures based on certain assumptions. The structures of thus obtained manipulators are examined and limb types were determined. These limb types were analyzed using screw theory. The instantaneous mobility of the manipulators and the motion characteristics of the moving platforms are tabulated. The finite mobility analysis of one of the manipulators is performed using a software package as an example. Among several different 3-UPU PM architectures, 118 novel 3-UPU PMs with non-parasitic 3-degrees-of-freedom are significantly important. The classified 3-UPU PMs with determined motion characteristics can be used by researchers as a design alternative for their specific design task.
Effective motion planning of manipulator based on SDPS-RRTConnect
Authors: Xu Junxiang , Wang Jiwu 
Published: October 2021

AbstractIn order to improve the speed of motion planning, this paper proposes an improved RRTConnect algorithm (SDPS-RRTConnect) based on sparse dead point saved strategy. Combining sparse expansion strategy and dead point saved strategy, the algorithm can reduce the number of collision detection, fast convergence, avoid falling into local minimum, and ensure the completeness of search space. The algorithm is simulated in different environments. The results show that in complex environments, the sparse dead point saved strategy plays a good effect. In simple environments, the greedy connection strategy works well. Compared with the standard RRT algorithm, the standard RRTConnect algorithm, and the SDPS-RRT algorithm, the SDPS-RRTConnect algorithm has the shortest planning time, and it is suitable for both simple and complex environments. The 500 experiments show that the algorithm has strong robustness. The actual robot experiments show that the path planned by SDPS-RRTConnect algorithm is effective.
BRIQUETAGE AND BRINE: LIVING AND WORKING AT THE CLASSIC MAYA SALT WORKS OF EK WAY NAL, BELIZE
Authors: McKillop Heather , Sills E. Cory 
Published: October 2021

AbstractSystematic flotation survey and spatial analysis of artifacts at the submerged salt work of Ek Way Nal reveal evidence of a residence, salt kitchens, and additional activities. Ek Way Nal is one of 110 salt works associated with a Late to Terminal Classic (A.D. 600–900) salt industry known as the Paynes Creek Salt Works. Wooden posts that form the walls of 10 buildings are remarkably preserved in a peat bog below the sea floor providing an opportunity to examine surface artifacts in relation to buildings. Numerous salt kitchens have been located at the Paynes Creek Salt Works by evidence of abundant briquetage—pottery associated with boiling brine over fires to make salt. As one of the largest salt works with 10 buildings, there is an opportunity to examine variability in building use. Systematic flotation survey over the site and flagging and mapping individual artifacts and posts provide evidence that the Ek Way Nal salt makers had a residence near the salt kitchens, along with evidence of salting fish for subsistence or surplus household production. The results are compared with ethnographic evidence from Sacapulas and other salt works.
FUNCTIONAL CALCULI FOR SECTORIAL OPERATORS AND RELATED FUNCTION THEORY
Authors: Batty Charles , Gomilko Alexander , Tomilov Yuri 
Published: October 2021

AbstractWe construct two bounded functional calculi for sectorial operators on Banach spaces, which enhance the functional calculus for analytic Besov functions, by extending the class of functions, generalising and sharpening estimates and adapting the calculus to the angle of sectoriality. The calculi are based on appropriate reproducing formulas, they are compatible with standard functional calculi and they admit appropriate convergence lemmas and spectral mapping theorems. To achieve this, we develop the theory of associated function spaces in ways that are interesting and significant. As consequences of our calculi, we derive several well-known operator norm estimates and provide generalisations of some of them.
Simulated herbicide spray retention on floating aquatic plants as affected by carrier volume and adjuvant type
Authors: Sperry Benjamin P. , Mudge Christopher R. , Getsinger Kurt D. 
Published: October 2021

AbstractFoliar delivery of herbicides is a common means for plant management in aquatic environments. Though this technique is decades old, little is known about vegetative spray retention relative to this application method. A more complete understanding of maximizing herbicide retention could lead to improved plant management while simultaneously decreasing pesticide load in aquatic environments. Therefore outdoor mesocosm experiments were conducted in 2020 to evaluate the effect of adjuvant type on foliar spray retention in waterhyacinth [Eichhornia crassipes (Mart.) Solms]. Additionally, the effect of carrier volume on spray retention in waterhyacinth, waterlettuce (Pistia stratiotes L.), and giant salvinia (Salvinia molesta D.S. Mitchell) was documented. Spray deposition did not differ among the nine adjuvants tested; however, spray retention was reduced 6% to 11% when an adjuvant was excluded from the spray solution. The effect of carrier volume on spray retention in waterhyacinth, waterlettuce, and giant salvinia was also investigated. Decreases in spray retention were most sensitive to increased carrier volume in waterhyacinth, followed by giant salvinia and waterlettuce. Among species, spray retention potential, as determined by intercept estimates, was greatest in waterlettuce and giant salvinia regardless of carrier volume. Asymptotes estimates for waterhyacinth, waterlettuce, and giant salvinia were 33%, 46%, and 79% spray retention, respectively. In other words, spray retention was the lowest and remained relatively constant at these values for the high carrier volumes tested (935 and 1,870 L ha−1), which were likely due to the presence of pubescence on leaves and flatter leaf architecture represented by waterlettuce and giant salvinia compared to the glabrous vertical leaves of waterhyacinth. Future research will evaluate these concepts under field conditions.
A Case Study: Evaluation of PAFOG One‐D Model With Advection in Simulations of Fog/Stratus From C‐FOG Experiment
Authors: Chen Changshuo , Zhang Minghong , Perrie Will , Chang Rachel , Gultepe Ismail , Fernando Harindra J. S. , Chen Xianyao 
Published: October 2021

AbstractFog simulation is a challenge due to the complex microphysical, radiative, and turbulent processes. C‐FOG (Coastal Fog) was a comprehensive experiment which aimed to improve our understanding and forecasting skill of fog in coastal areas of Atlantic Canada. An intermittent fog event, containing two fog periods occurring over the Grand Banks of Newfoundland beginning at 00:00 UTC on September 13, 2018, is investigated in this study. Our approach is to apply the Weather Research and Forecasting (WRF) model, in conjunction with a 1‐D model for the atmospheric boundary layer, PAFOG (Parameterized Fog model). Model results are evaluated against observations collected onboard the research vessel Hugh R. Sharp. We introduced an advection term to PAFOG to investigate the ability of the model to simulate the two‐level fog/stratus system and we developed a set of sensitivity experiments. Results suggest that PAFOG with the advection terms calculated from ERA5 shows a good ability to simulate intermittent fog caused by fog lifting as stratus, and by comparison, outperform results with advection terms derived from WRF simulations. Regarding the latter, in an experiment with high vertical resolution, WRF gives a delayed fog event, whereas with low vertical resolution, WRF gives descending stratus. Our results provide a possible methodology to study the detailed structure of fog/stratus using PAFOG, a sounding profile as initial conditions, and advection profiles derived from ERA5, with low computing cost.
Does interpregnancy BMI change affect the risk of complications in the second pregnancy? Analysis of pooled data from Aberdeen, Finland and Malta
Authors: McClurg Dylan Peter , Gissler Mika , Gatt Miriam , Wallace Jacqueline , Bhattacharya Sohinee 
Published: October 2021

Abstract
Objective
Weight management interventions during pregnancy have had limited success in reducing the risk of pregnancy complications. Focus has now shifted to pre-pregnancy counselling to optimise body weight before subsequent conception. We aimed to assess the effect of interpregnancy body mass index (BMI) change on the risk of perinatal complications in the second pregnancy.

Methods
A cohort study was performed using pooled maternity data from Aberdeen, Finland and Malta. Women with a BMI change of ±2 kg/m2 between their first and second pregnancies were compared with those who were BMI stable (remained within ±2 kg/m2). Outcomes assessed included pre-eclampsia (PE), intrauterine growth restriction (IUGR), preterm birth, birth weight, and stillbirth in the second pregnancy. We also assessed the effect of unit change in BMI for PE and IUGR. Logistic regression was used to calculate odds ratios with 95% confidence intervals.

Results
An increase of ≥2 kg/m2 between the first two pregnancies increased the risk of PE (1.66 (1.49–1.86)) and high birthweight (>4000 g) (1.06 (1.03–1.10)). A reduction of ≥2 kg/m2 increased the chance of IUGR (1.15 (1.01–1.31)) and preterm birth (1.14 (1.01–1.30)), while reducing the risk of instrumental delivery (0.75 (0.68–0.85)) and high birthweight (0.93 (0.87–0.98)). Reducing BMI did not significantly decrease PE risk in women with obesity or those with previous PE. A history of PE or IUGR in the first pregnancy was the strongest predictor of recurrence independent of interpregnancy BMI change (5.75 (5.30–6.24) and (7.44 (6.71–8.25), respectively).

Conclusion
Changes in interpregnancy BMI have a modest impact on the risk of high birthweight, PE and IUGR in contrasting directions. However, a prior history of PE and IUGR is the dominant predictor of recurrence at second pregnancy.
Multichannel esophageal signals to monitor respiratory rate in preterm infants
Authors: Bürgin Corine , Simmen Patrizia , Gupta Nishant , Suter Lilian , Kreuzer Samuel , Haeberlin Andreas , Schulzke Sven M. , Trachsel Daniel , Niederhauser Thomas , Jost Kerstin 
Published: October 2021

AbstractBackgroundApnea of prematurity cannot be reliably measured with current monitoring techniques. Instead, indirect parameters such as oxygen desaturation or bradycardia are captured. We propose a Kalman filter-based detection of respiration activity and hence apnea using multichannel esophageal signals in neonatal intensive care unit patients.MethodsWe performed a single-center observational study with moderately preterm infants. Commercially available nasogastric feeding tubes containing multiple electrodes were used to capture signals with customized software. Multichannel esophageal raw signals were manually annotated, processed using extended Kalman filter, and compared with standard monitoring data including chest impedance to measure respiration activity.ResultsOut of a total of 405.4 h captured signals in 13 infants, 100 episodes of drop in oxygen saturation or heart rate were examined. Median (interquartile range) difference in respiratory rate was 0.04 (−2.45 to 1.48)/min between esophageal measurements annotated manually and with Kalman filter and −3.51 (−7.05 to −1.33)/min when compared to standard monitoring, suggesting an underestimation of respiratory rate when using the latter.ConclusionsKalman filter-based estimation of respiratory activity using multichannel esophageal signals is safe and feasible and results in respiratory rate closer to visual annotation than that derived from chest impedance of standard monitoring.
Molecular basis of a bacterial-amphibian symbiosis revealed by comparative genomics, modeling, and functional testing
Authors: Brunetti Andrés E , Bunk Boyke , Lyra Mariana L , Fuzo Carlos A , Marani Mariela M , Spröer Cathrin , Haddad Célio F B , Lopes Norberto P , Overmann Jörg 
Published: October 2021

Abstract
The molecular bases for the symbiosis of the amphibian skin microbiome with its host are poorly understood. Here, we used the odor-producer Pseudomonas sp. MPFS and the treefrog Boana prasina as a model to explore bacterial genome determinants and the resulting mechanisms facilitating symbiosis. Pseudomonas sp. MPFS and its closest relatives, within a new clade of the P. fluoresens Group, have large genomes and were isolated from fishes and plants, suggesting environmental plasticity. We annotated 16 biosynthetic gene clusters from the complete genome sequence of this strain, including those encoding the synthesis of compounds with known antifungal activity and of odorous methoxypyrazines that likely mediate sexual interactions in Boana prasina. Comparative genomics of Pseudomonas also revealed that Pseudomonas sp. MPFS and its closest relatives have acquired specific resistance mechanisms against host antimicrobial peptides (AMPs), specifically two extra copies of a multidrug efflux pump and the same two-component regulatory systems known to trigger adaptive resistance to AMPs in P. aeruginosa. Subsequent molecular modeling indicated that these regulatory systems interact with an AMP identified in Boana prasina through the highly acidic surfaces of the proteins comprising their sensory domains. In agreement with a symbiotic relationship and a highly selective antibacterial function, this AMP did not inhibit the growth of Pseudomonas sp. MPFS but inhibited the growth of another Pseudomonas species and Escherichia coli in laboratory tests. This study provides deeper insights into the molecular interaction of the bacteria-amphibian symbiosis and highlights the role of specific adaptive resistance toward AMPs of the hosts.
Gadd45g initiates embryonic stem cell differentiation and inhibits breast cell carcinogenesis
Authors: Zhang Xinbao , Li Yuting , Ji Junxiang , Wang Xin , Zhang Meng , Li Xiangfen , Zhang Yan , Zhu Zhenhua , Ye Shou-Dong , Wang Xiaoxiao 
Published: October 2021

AbstractMany self-renewal-promoting factors of embryonic stem cells (ESCs) have been implicated in carcinogenesis, while little known about the genes that direct ESCs exit from pluripotency and regulate tumor development. Here, we show that the transcripts of Gadd45 family genes, including Gadd45a, Gadd45b, and Gadd45g, are gradually increased upon mouse ESC differentiation. Upregulation of Gadd45 members decreases cell proliferation and induces endodermal and trophectodermal lineages. In contrast, knockdown of Gadd45 genes can delay mouse ESC differentiation. Mechanistic studies reveal that Gadd45g activates MAPK signaling by increasing expression levels of the positive modulators of this pathway, such as Csf1r, Igf2, and Fgfr3. Therefore, inhibition of MAPK signaling with a MEK specific inhibitor is capable of eliminating the differentiation phenotype caused by Gadd45g upregulation. Meanwhile, GADD45G functions as a suppressor in human breast cancers. Enforced expression of GADD45G significantly inhibits tumor formation and breast cancer metastasis in mice through limitation of the propagation and invasion of breast cancer cells. These results not only expand our understanding of the regulatory network of ESCs, but also help people better treatment of cancers by manipulating the prodifferentiation candidates.
Cascade screening for beta-thalassaemia in Pakistan: relatives’ experiences of a decision support intervention in routine practice
Authors: Ahmed Shenaz , Jafri Hussain , Faran Muhammed , Ahmed Wajeeha Naseer , Rashid Yasmin , Ehsan Yasmin , Ahmed Mushtaq 
Published: October 2021

AbstractLow uptake of cascade screening for βeta-thalassaemia major (β-TM) in the ‘Punjab Thalassaemia Prevention Project’ (PTPP) in Pakistan led to the development of a ‘decision support intervention for relatives’ (DeSIRe). This paper presents the experiences of relatives of children with β-TM of the DeSIRe following its use by PTPP field officers (FOs) in routine clinical practice. Fifty-four semi-structured qualitative interviews were conducted (April to June 2021) with relatives in seven cities in the Punjab province (Lahore, Sheikhupura, Nankana Sahab, Kasur, Gujranwala, Multan and Faisalabad). Thematic analysis shows that participants were satisfied with the content of the DeSIRe and its delivery by the FOs in a family meeting. They understood that the main purpose of the DeSIRe was to improve their knowledge of β-TM and its inheritance, and to enable them to make decisions about thalassaemia carrier testing, particularly before marriage. Participants also raised concerns about the stigma of testing positive; however, they believed the DeSIRe was an appropriate intervention, which supported relatives to make informed decisions. Our findings show that the DeSIRe is appropriate for use by healthcare professionals in routine practice in a low-middle income country, and has the potential to facilitate shared decision making about cascade screening for thalassaemia. Further research is needed to prove the efficacy of the DeSIRe.
PbrRALF2-elicited reactive oxygen species signaling is mediated by the PbrCrRLK1L13-PbrMPK18 module in pear pollen tubes
Authors: Kou Xiaobing , Sun Jiangmei , Wang Peng , Wang Danqi , Cao Peng , Lin Jing , Chang Youhong , Zhang Shaoling , Wu Juyou 
Published: October 2021

Abstract
Rapid alkalinization factors (RALFs) are cysteine-rich peptides that play important roles in a variety of biological processes, such as cell elongation and immune signaling. Recent studies in Arabidopsis have shown that RALFs regulate pollen tube growth via plasma membrane receptor-like kinases (RLKs). However, the downstream signal transduction mechanisms of RLKs in pollen tubes are unknown. Here, we identified PbrRALF2, a pear (Pyrus bretschneideri) pollen RALF peptide that inhibits pollen tube growth. We found that PbrRALF2 interacts with a malectin-like domain-containing RLK, PbrCrRLK1L13. The relative affinity between PbrRALF2 and PbrCrRLK1L13 was at the submicromolar level, which is consistent with the values of ligand–receptor kinase pairs and the physiological concentration for PbrRALF2-mediated inhibition of pollen tube growth. After binding to its extracellular domain, PbrRALF2 activated the phosphorylation of PbrCrRLK1L13 in a dose-dependent manner. We further showed that the MAP kinase PbrMPK18 is a downstream target of PbrCrRLK1L13 that mediates PbrRALF2-elicited reactive oxygen species (ROS) production. The excessive accumulation of ROS inhibits pollen tube growth. We show that MPK acts as a mediator for CrRLK1L to stimulate ROS production, which might represent a general mechanism by which RALF and CrRLK1L function in signaling pathways.