bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Continuum Deformation Approach for Growth Analysis of COVID-19 in the United States
Authors: Hemmati Sadra , Rastgoftar Hossein 
Published: September 2021

AbstractThe COVID-19 global pandemic has significantly impacted every aspect of life all over the world. The United States is reported to have suffered more than 20% of the global casualties from this pandemic. It is imperative to investigate the growth dynamics of the disease in the US based on varying geographical and governmental factors that best manifest itself in each State of the Country. This paper utilizes a hybrid machine learning and continuum deformation-based approach for analyzing the stability and growth rate of the pandemic. To this end, principal stress values of the pandemic continuum body are obtained using Mohr’s Circle method and overlapping, moving windows of data are analysed successively. This helps in finding the correlations between the growth rate and Governments’ action/Public’s reaction. Government actions include “state of emergency”, “shelter at place”, and “phase declarations”. We also consider the vaccination rate milestones, which shows us the coordinated Governments’ action/Public’s reaction. Finally, a number of recommendations are made to the Governments and people for better management of future pandemics.
Pituitary hormones are specifically expressed in trigeminal sensory neurons and contribute to pain responses in the trigeminal system
Authors: Hovhannisyan Anahit H. , Son Hyeonwi , Mecklenburg Jennifer , Barba-Escobedo Priscilla Ann , Tram Meilinn , Gomez Ruben , Shannonhouse John , Zou Yi , Weldon Korri , Ruparel Shivani , Lai Zhao , Tumanov Alexei V. , Kim Yu Shin , Akopian Armen N. 
Published: September 2021

AbstractTrigeminal (TG), dorsal root (DRG), and nodose/jugular (NG/JG) ganglia each possess specialized and distinct functions. We used RNA sequencing of two-cycle sorted Pirt-positive neurons to identify genes exclusively expressing in L3–L5 DRG, T10-L1 DRG, NG/JG, and TG mouse ganglion neurons. Transcription factor Phox2b and Efcab6 are specifically expressed in NG/JG while Hoxa7 is exclusively present in both T10-L1 and L3–L5 DRG neurons. Cyp2f2, Krt18, and Ptgds, along with pituitary hormone prolactin (Prl), growth hormone (Gh), and proopiomelanocortin (Pomc) encoding genes are almost exclusively in TG neurons. Immunohistochemistry confirmed selective expression of these hormones in TG neurons and dural nerves; and showed GH expression in subsets of TRPV1+ and CGRP+ TG neurons. We next examined GH roles in hypersensitivity in the spinal versus trigeminal systems. Exogenous GH produced mechanical hypersensitivity when injected intrathecally, but not intraplantarly. GH-induced thermal hypersensitivity was not detected in the spinal system. GH dose-dependently generated orofacial and headache-like periorbital mechanical hypersensitivity after administration into masseter muscle and dura, respectively. Periorbital mechanical hypersensitivity was reversed by a GH receptor antagonist, pegvisomant. Overall, pituitary hormone genes are selective for TG versus other ganglia somatotypes; and GH has distinctive functional significance in the trigeminal versus spinal systems.
Evidence of weak Anderson localization revealed by the resistivity, transverse magnetoresistance and Hall effect measured on thin Cu films deposited on mica
Authors: Díaz Eva , Herrera Guillermo , Oyarzún Simón , Munoz Raul C. 
Published: September 2021

AbstractWe report the resistivity of 5 Cu films approximately 65 nm thick, measured between 5 and 290 K, and the transverse magnetoresistance and Hall effect measured at temperatures 5 K < T < 50 K. The mean grain diameters are D = (8.9, 9.8, 20.2, 31.5, 34.7) nm, respectively. The magnetoresistance signal is positive in samples where D > L/2 (where L = 39 nm is the electron mean free path in the bulk at room temperature), and negative in samples where D < L/2. The sample where D = 20.2 nm exhibits a negative magnetoresistance at B < 2 Tesla and a positive magnetoresistance at B > 3 Tesla. A negative magnetoresistance in Cu films has been considered evidence of charge transport involving weak Anderson localization. These experiments reveal that electron scattering by disordered grain boundaries found along L leads to weak Anderson localization, confirming the localization phenomenon predicted by the quantum theory of resistivity of nanometric metallic connectors. Anderson localization becomes a severe obstacle for the successful development of the circuit miniaturization effort pursued by the electronic industry, for it leads to a steep rise in the resistivity of nanometric metallic connectors with decreasing wire dimensions (D < L/2) employed in the design of Integrated Circuits.
Autofluorescence-spectral imaging as an innovative method for rapid, non-destructive and reliable assessing of soybean seed quality
Authors: Barboza da Silva Clíssia , Oliveira Nielsen Moreira , de Carvalho Marcia Eugenia Amaral , de Medeiros André Dantas , de Lima Nogueira Marina , dos Reis André Rodrigues 
Published: September 2021

AbstractIn the agricultural industry, advances in optical imaging technologies based on rapid and non-destructive approaches have contributed to increase food production for the growing population. The present study employed autofluorescence-spectral imaging and machine learning algorithms to develop distinct models for classification of soybean seeds differing in physiological quality after artificial aging. Autofluorescence signals from the 365/400 nm excitation-emission combination (that exhibited a perfect correlation with the total phenols in the embryo) were efficiently able to segregate treatments. Furthermore, it was also possible to demonstrate a strong correlation between autofluorescence-spectral data and several quality indicators, such as early germination and seed tolerance to stressful conditions. The machine learning models developed based on artificial neural network, support vector machine or linear discriminant analysis showed high performance (0.99 accuracy) for classifying seeds with different quality levels. Taken together, our study shows that the physiological potential of soybean seeds is reduced accompanied by changes in the concentration and, probably in the structure of autofluorescent compounds. In addition, altering the autofluorescent properties in seeds impact the photosynthesis apparatus in seedlings. From the practical point of view, autofluorescence-based imaging can be used to check modifications in the optical properties of soybean seed tissues and to consistently discriminate high-and low-vigor seeds.
Strain and sex dependent effects of isolation housing relative to environmental enrichment on operant sensation seeking in mice
Authors: Dickson Price E. , Mittleman Guy 
Published: September 2021

AbstractSensation seeking is a multidimensional phenotype that predicts the development of drug addiction in humans and addiction-like drug seeking in rodents. Several lines of evidence suggest that chronic stress increases sensation seeking and addiction-like drug seeking through common genetic mechanisms. Discovery and characterization of these mechanisms would reveal how chronic stress interacts with the genome to influence sensation seeking and how drugs of abuse hijack these fundamental reward mechanisms to drive addiction. To this end, we tested the hypothesis that chronic isolation housing stress (relative to environmental enrichment) influences operant sensation seeking as a function of strain, sex, or their interaction. To determine if the BXD recombinant inbred panel could be used to identify genetic and epigenetic mechanisms underlying any identified gene-by-environment interactions, we used mice from the two BXD founder strains. Following 10 weeks of differential housing, we assessed operant sensation seeking using several reinforcement schedules. The primary finding from this study was that DBA/2J but not C57BL/6J mice were significantly vulnerable to an isolation-induced increase (relative to environmental enrichment) in sensation seeking during extinction when the sensory reward was no longer available; this effect was significantly more robust in females. These data reveal a previously unknown isolation-induced effect on extinction of operant sensation seeking that is sex-dependent, addiction-relevant, and that can be dissected using the BXD recombinant inbred panel.
Individual alpha peak frequency is slower in schizophrenia and related to deficits in visual perception and cognition
Authors: Ramsay Ian S. , Lynn Peter A. , Schermitzler Brandon , Sponheim Scott R. 
Published: September 2021

AbstractThe brain at rest generates cycles of electrical activity that have been shown to be abnormal in people with schizophrenia. The alpha rhythm (~ 10 Hz) is the dominant resting state electrical cycle and each person has a propensity toward a particular frequency of oscillation for this rhythm. This individual alpha peak frequency (IAPF) is hypothesized to be central to visual perceptual processes and may have downstream influences on cognitive functions such as attention, working memory, or problem solving. In the current study we sought to determine whether IAPF was slower in schizophrenia, and whether lower IAPF predicted deficits in visual perception and cognition that are often observed in schizophrenia. Eyes-closed resting state EEG activity, visual attention, and global cognitive functioning were assessed in individuals with schizophrenia (N = 104) and a group of healthy controls (N = 101). Compared to controls, the schizophrenia group showed slower IAPF and was associated with poorer discrimination of visual targets and nontargets on a computerized attention task, as well as impaired global cognition measured using neuropsychological tests across groups. Notably, disruptions in visual attention fully mediated the relationship between IAPF and global cognition across groups. The current findings demonstrate that slower alpha oscillatory cycling accounts for global cognitive deficits in schizophrenia by way of impairments in perceptual discrimination measured during a visual attention task.
Clinical features and prognosis of polypoidal choroidal vasculopathy with different morphologies of branching vascular network on optical coherence tomography angiography
Authors: Ma Shang-Te , Huang Chu-Hsuan , Chang Yun-Chia , Lai Tso-Ting , Hsieh Yi-Ting , Ho Tzyy-Chang , Yang Chung-May , Cheng Cheng-Guo , Yang Chang-Hao 
Published: September 2021

AbstractThis study highlights the clinical features and treatment response of polypoidal choroidal vasculopathy (PCV) among three different branching vascular network (BVN) morphologies in optical coherence tomography angiography (OCTA), and further correlates the BVN features with those under fluorescent angiography (FA) and indocyanine green angiography (ICGA). In total, we reviewed 70 eyes with PCV followed up for > 12 months. OCTA, ICGA and FA images were obtained at baseline and post-treatments. BVN was assessed using OCTA and divided into three types by a previously described BVN classification: type 1 (trunk), type 2 (glomeruli), and type 3 (stick). At baseline, type 1 BVN had the poorest vision and thinnest subfoveal choroidal thickness (SFCT), whereas type 3 had the best vision and thickest SFCT. The aforementioned trend sustained after treatments. Each BVN morphology in OCTA showed typical features in FA + ICGA and encompassed significant correlation (p = 0.004). In conclusion, OCTA is an innovative imaging tool for the detection and classification of BVN in PCV. Furthermore, OCTA has advantages of being noninvasive and free of systemic toxicities. The BVN can be divided into three types based on morphological characteristics in OCTA, which play crucial roles in clinical presentations and treatment outcomes.
Toll-like receptor 1 as a possible target in non-alcoholic fatty liver disease
Authors: Baumann Anja , Nier Anika , Hernández-Arriaga Angélica , Brandt Annette , Lorenzo Pisarello Maria J. , Jin Cheng J. , Pilar Esther , Camarinha-Silva Amélia , Schattenberg Jörn M. , Bergheim Ina 
Published: September 2021

AbstractToll-like receptors (TLRs) in the liver compartment have repeatedly been attributed to the development of non-alcoholic fatty liver disease (NAFLD). Knowledge on TLR expression in blood cells and their relation to intestinal microbiota and NAFLD development is limited. Here, we determined TLR expression patterns in peripheral blood mononuclear cells (PBMCs) of NAFLD patients and controls, their relation to intestinal microbiota and the impact of TLRs found altered in NAFLD development. Markers of intestinal permeability in blood andTLRmRNA expression in PBMCs were determined in 37 NAFLD patients and 15 age-matched healthy controls. Fecal microbiota composition was evaluated in 21 NAFLD patients and 9 controls using 16S rRNA gene amplicon sequencing. Furthermore,TLR1−/−and C57BL/6 mice (n = 5–6/group) were pair-fed a liquid control or a fat-, fructose- and cholesterol-rich diet. Intestinal microbiota composition and markers of intestinal permeability like zonulin and bacterial endotoxin differed significantly between groups with the latter markers being significantly higher in NAFLD patients. Expression ofTLR1-8and10mRNA was detectable in PBMCs; however, onlyTLR1expression, being higher in NAFLD patients, were significantly positively correlated with the prevalence ofHoldemanellagenus while negative correlations were found withGemmigerandRuminococcusgenera.TLR1−/−mice were significantly protected from the development of diet-induced NAFLD when compared to wild-type mice. While intestinal microbiota composition and permeability differed significantly between NAFLD patients and healthy subjects, in PBMCs, onlyTLR1expression differed between groups. Still, targeting these alterations might be a beneficial approach in the treatment of NAFLD in some patients.
The meso-fracturing mechanism of marble under unloading confining pressure paths and constant axial stress
Authors: Sun Linna , Zhang Liming , Cong Yu , Song Yaduo , He Keqiang 
Published: September 2021

AbstractFailure tests on marble during unloading confining-pressure under constant axial stress and simulations with the particle flow code were performed. The influence mechanism of the unloading rate of the confining pressure, initial unloading stress, and confining pressure on the failure characteristics of, and crack propagation in, marble was studied. By using the trial-and-error method, the conversion relationship between the unloading rates of confining pressures in laboratory tests and numerical simulations was ascertained. Micro-cracks formed in the unloading process of confining pressure are dominated by tension cracks, accompanied by shear cracks. The propagation of shear cracks lags that of tension cracks. As the confining pressure is increased, more cracks occur upon failure of the samples. The proportion of shear cracks increases while that of tension cracks decreases. The failure mode of samples undergoes a transition from shear-dominated failure to conjugated shear failure.
The mitochondrial calcium uniporter promotes arrhythmias caused by high-fat diet
Authors: Joseph Leroy C. , Reyes Michael V. , Homan Edwin A. , Gowen Blake , Avula Uma Mahesh R. , Goulbourne Chris N. , Wan Elaine Y. , Elrod John W. , Morrow John P. 
Published: September 2021

AbstractObesity and diabetes increase the risk of arrhythmia and sudden cardiac death. However, the molecular mechanisms of arrhythmia caused by metabolic abnormalities are not well understood. We hypothesized that mitochondrial dysfunction caused by high fat diet (HFD) promotes ventricular arrhythmia. Based on our previous work showing that saturated fat causes calcium handling abnormalities in cardiomyocytes, we hypothesized that mitochondrial calcium uptake contributes to HFD-induced mitochondrial dysfunction and arrhythmic events. For experiments, we used mice with conditional cardiac-specific deletion of the mitochondrial calcium uniporter (Mcu), which is required for mitochondrial calcium uptake, and littermate controls. Mice were used for in vivo heart rhythm monitoring, perfused heart experiments, and isolated cardiomyocyte experiments. MCU KO mice are protected from HFD-induced long QT, inducible ventricular tachycardia, and abnormal ventricular repolarization. Abnormal repolarization may be due, at least in part, to a reduction in protein levels of voltage gated potassium channels. Furthermore, isolated cardiomyocytes from MCU KO mice exposed to saturated fat are protected from increased reactive oxygen species (ROS), mitochondrial dysfunction, and abnormal calcium handling. Activation of calmodulin-dependent protein kinase (CaMKII) corresponds with the increase in arrhythmias in vivo. Additional experiments showed that CaMKII inhibition protects cardiomyocytes from the mitochondrial dysfunction caused by saturated fat. Hearts from transgenic CaMKII inhibitor mice were protected from inducible ventricular tachycardia after HFD. These studies identify mitochondrial dysfunction caused by calcium overload as a key mechanism of arrhythmia during HFD. This work indicates that MCU and CaMKII could be therapeutic targets for arrhythmia caused by metabolic abnormalities.
Incidence, characteristics and risk factors of thromboembolic events in East Asian patients with BCR-ABL1 negative myeloproliferative neoplasms
Authors: Kim Jinyong , Byun Ja Min , Hong Junshik , Koh Youngil , Shin Dong-Yeop , Kim Inho , Yoon Sung-Soo , Park Hyunkyung , Bang Soo-Mee 
Published: September 2021

AbstractThe vascular complications have been a major cause of morbidity and mortality among all subtypes of BCR-ABL1 negative myeloproliferative neoplasms (MPN), but the ethnicity-specific data was limited. We therefore conducted a multi-center retrospective, longitudinal cohort study to evaluate the incidence, characteristics and risk factors of thromboembolic events of MPN patients. Of 256 patients, 27.3% experienced thromboembolic events, majority of which occurred before or within 12 months of MPN diagnosis. The multivariable Cox proportional analyses identified leukocytosis (HR 2.67, 95% CI 1.36–5.24, q = 0.004) and history of thrombosis (HR 9.68, 95% CI 2.00–46.88, q = 0.005) as the risk factors for thromboembolism. In subgroup analysis of polycythemia vera and hemoglobin concentration (HR 1.97, 95% CI 1.28–3.04, q = 0.002) appeared to be a significant risk factor of thrombosis, along with age and thrombosis history. In essential thrombocythemia, severity of the established IPSET score was closely correlated with the frequency of thromboembolic events. In primary myelofibrosis, history of thrombosis was associated with thrombosis events (HR 13.85, 95% CI 1.2–159.5, q = 0.035). Overall survival was worse in patients who experienced thromboembolic events. Our study highlighted the importance of recognizing high risk patients and implementing personalized intervention.
Solar Orbiter observations of the Kelvin-Helmholtz waves in the solar wind
Authors: Kieokaew R. , Lavraud B. , Yang Y. , Matthaeus W. H. , Ruffolo D. , Stawarz J. E. , Aizawa S. , Foullon C. , Génot V. , Pinto R. F. , Fargette N. , Louarn P. , Rouillard A. , Fedorov A. , Penou E. , Owen C. J. , Horbury T. S. , O’Brien H. , Evans V. , Angelini V. 
Published: September 2021

Context.The Kelvin-HeImholtz (KH) instability is a nonlinear shear-driven instability that develops at the interface between shear flows in plasmas. KH waves have been inferred in various astrophysical plasmas, and have been observed in situ at the magnetospheric boundaries of solar-system planets and through remote sensing at the boundaries of coronal mass ejections.Aims.KH waves are also expected to develop at flow shear interfaces in the solar wind. While they were hypothesized to play an important role in the mixing of plasmas and in triggering solar wind fluctuations, their direct and unambiguous observation in the solar wind was still lacking.Methods.We report in situ observations of quasi-periodic magnetic and velocity field variations plausibly associated with KH waves using Solar Orbiter during its cruise phase. They are found in a shear layer in the slow solar wind in the close vicinity of the heliospheric current sheet. An analysis was performed to derive the local configuration of the waves. A 2D magnetohydrodynamics simulation was also set up with approximate empirical values to test the stability of the shear layer. In addition, magnetic spectra of the event were analyzed.Results.We find that the observed conditions satisfy the KH instability onset criterion from the linear theory analysis, and its development is further confirmed by the simulation. The current sheet geometry analyses are found to be consistent with KH wave development, albeit with some limitations likely owing to the complex 3D nature of the event and solar wind propagation. Additionally, we report observations of an ion jet consistent with magnetic reconnection at a compressed current sheet within the KH wave interval. The KH activity is found to excite magnetic and velocity fluctuations with power law scalings that approximately followk−5/3andk−2.8in the inertial and dissipation ranges, respectively. Finally, we discuss reasons for the lack of in situ KH wave detection in past data.Conclusions.These observations provide robust evidence of KH wave development in the solar wind. This sheds new light on the process of shear-driven turbulence as mediated by the KH waves with implications for the driving of solar wind fluctuations.