bluuebunny/crossref_metadata_2025_split huggingface dataset:
OCT4 Promotes Ovarian Cancer Cell Metastasis and Angiogenesis via Modulating VEGFR2/LRPPRC Pathway
Authors: Wu Dongling , Xie Weiwei , Wang Husheng , Chen Wei , Chen Xin , Sun Huizhen 
Published: October 2021

Abstract
Background: Due to its high ability of metastasis, ovarian cancer remains the most lethal gynecological malignancy, yet its underlying mechanism remains unconfirmed. Objectives: The main purpose is to probe into the role and regulation mechanism of octamer-binding transcription factor 4 (OCT4) in angiogenesis and metastasis in ovarian cancer.Methods: Immunohistochemistry (IHC) and immunofluorescence in epithelial ovarian cancer specimens and benign ovarian tumor samples were performed, followed by RNA-sequencing and examination of angiogenesis, cell migration and invasion in OCT4 knockdown cell lines and the controls. Co-Immunoprecipitation (Co-IP), mass spectrometry, immunoblotting, immunofluorescence and chromatin immunoprecipitation (ChIP) analyses were conducted along with models of zebrafishes and nude mice of transplanted tumors to gain insights into the specific functions and mechanisms of action of OCT4 in ovarian cancer.Results: Firstly, we discovered that OCT4 expression was enhanced in ovarian cancer tissues significantly, especially in the metastatic lesions, indicating that OCT4 might be a key for the metastasis of ovarian cancer. Furtherly, we observed and verified that OCT4 promoted cell migration and invasion, and induced angiogenesis in vitro and in vivo. Mechanistically, OCT4 modulated the transcription of leucine‑rich PPR motif‑containing protein (LRPPRC)，and furtherly interacted with vascular endothelial growth factor receptor 2 (VEGFR2)/LRPPRC complex and ultimately triggered the downstream FAK/AKT signaling pathway . Conclusions: Together, through models of ovarian cancer cells, zebrafishes and tumor-transplanted mice, this study highlighted the importance of OCT4/LRPPRC/VEGFR2 signaling axis in metastasis of ovarian cancer and angiogenesis. Thus, our finding supplied a potential novel molecular-targeted approach for the treatment of ovarian cancer.
The High-Grade Glial Component Of Pediatric Primary Anaplastic Ganglioglioma Characterized Astroblastoma-Like Pseudorosettes With BRAFV600E Mutation And Deletion Of CDKN2A/B, PTEN, And BMPR1A: A Case Report
Authors: Zejun Duan , Kun Yao , Jing Feng , Xueling Qi 
Published: October 2021

Abstract
Background: Ganglioglioma (GG) is a low-grade mixed neuronal-glial tumor which is the most common type of long-term epilepsy-associated tumors (LEATs). However, primary anaplastic ganglioglioma (AGG) which composes of malignant changes is rare. Here, we report a case of pediatric primary AGG which is consisted of low-grade/begnin GG and high-grade glioma that was characterized by astroblastoma-like pseudorosettes. Case presentation: We describe a case of 4-year-old female who presented with medically refractory seizure for 14 months by a temporal mass. The patient underwent a gross total mass resection at the first surgery, and was only treated with antiepileptic therapy and followed by observation. After nine months, tumor recurrence was found. Followed by second operation, the patient was treated with chemotherapy (oral temozolomide and antiepileptic drugs) and local radiotherapy. At 58-month follow-up after the second operation, no epileptic seizures and tumor recurrence were found again. In the first sample, the tumor contained two different components. The major component presented the low-grade GG’s features of neoplastic glial cells and dysplastic ganglion cells. The minor component was a heterogeneous high-grade glioma characterized astroblastic-like pseudorosettes clusters with increased mitotic figure (about 4-6 per 10 high-power fields). CD34 staining was negative. BRAFV600E was positive in both components. In the recurrent sample, the heterogeneous high-grade glioma became the major component. The fuorescence in situ hybridization (FISH) of MN1 break-apart probe and MYB–QKI fusions probe were negative. BRAFV600E mutation, and deletion of CDKN2A/B, PTEN and BMPR1A were detected by targeted DNA sequencing. Conclusion: This case extends the histomorphologic spectrum and enriched genetic features of primary AGG in childhood. The high-grade glioma charactered astroblastoma-like pseudorosettes may be an important cause of tumor recurrence in a short period of time. Tumor gross total surgical resection and adjuvant chemoradiotherapy were important to achive an event-free survival.
Study of Bone Quality and Fracture Line Morphology of the Posterior Medial Humeral Calcar in Proximal Humeral Fractures
Authors: Xu Zheng , Xiang Ming , Yang Jinsong , Gao Xu , Cao Yi 
Published: October 2021

Abstract
Objective: To investigate the relationship between fracture line morphology, bone quality and fracture morphology of the posterior medial humeral calcar in proximal humeral fractures METHODS: CT data of patients with proximal humeral fractures diagnosed in our hospital from 06/2019 to 06/2021 were retrospectively analyzed to describe the map and coordinate analysis of the posterior medial humeral calcar fracture based on three-dimensional reconstruction, to create varus, valgus and normal groups according to the inclination angle of the humeral head, and to measure the bone mass of the posterior medial humeral calcar and perform statistical analysis.RESULTS: Sixty-two patients met the inclusion criteria, aged 15 to 72 years, there were 21 varus, 24 valgus and 17 normal types. Epiphyseal extension occurred most frequently posteriorly and medially, with the fracture line ending mostly posterior to the greater tuberosity. The thickness of the varus was (2.33 ± 0.47), valgus was (2.59 ± 0.33) and normal type was (2.69 ± 0.53). The T-test showed that the thickness of the bone in the varus was less than the other two types, and bone density and pinch angle were no statistically significant.CONCLUSION: There is no correlation between the trend of the posterior medial fracture line of the humeral calcar, bone density and internal, external rotation of the humeral head. The medial bone thickness after varus is less than that of valgus and normal fractures. The fracture line endpoint is mostly located posterior to the greater tuberosity.
Optimal Planning of the Mobile Cargo Ropeway Repair Strategy
Authors: Lagerev Alexander V. , Lagerev Igor A. 
Published: October 2021

Abstract
The focus of this research is to increase the reliability of mobile cargo ropeways formed by autonomous self-propelled transport units. The article deals with the development of a method for forming an effective technical and economic strategy for the restoration during planned repairs of those structural elements of transport units that can lead to critical failures of the ropeway. The method involves predicting the kinetics of the probability of failure-free operation of the ropeway during the entire life of its operation on the basis of predicting the failure-free operation of key elements of the transport units, the failure of which leads to an emergency disruption of the ropeway. In the process of integrating the system of Chapman-Kolmogorov differential equations, its periodic reformation is performed at the time of planned repairs, which allows us to take into account the need for a discrete change in the probability of failure-free operation of the restored structural elements. As a criterion for the optimality of the repairs strategy, the condition for obtaining the minimum total cost of repairs is used, while ensuring the average probability of failure-free operation. The formation of such an optimal strategy includes planning the schedules, number, time points, volume and cost of restoration work. The effectiveness of the repair strategy is determined by the total number of planned repairs and the minimum permissible probability of critical failure of structural elements. Conditions have been established under which further improvement of the level of ropeway reliability becomes an economically unprofitable task.
Development and Verification of a Model for Assessment of Risk Factors of Death in Floods
Authors: Yari Arezoo , Zarezadeh Yadolah , Rahimiforoushani Abbas , Ardalan Ali , Boubakran Mohsen Soufi , Bidarpoor Farzam , Ostadtaghizadeh Abbas 
Published: October 2021

Abstract
Background: Determination of the causes of flood-related deaths is the precondition for effective interventions aimed at the reduction of such deaths. There is a gap in the design and the development of a valid and reliable tool for measuring underlying factors of death in the flood.Methods: A complete set of causes of flood deaths was collected. After forming the pool of items, an initial questionnaire was designed and divided into two parts of objective and subjective factors. The questionnaire’s psychometric evaluation was performed for the subjective part.Results: At the design stage, the objective and subjective sections were designed. During the psychometric evaluation, the number of items was reduced. While measuring the content validity 13 questions were excluded. Finally, a 33-item questionnaire was developed in seven categories. In the confirmatory factor analysis, the KMO coefficient was higher than .05 for all constructs. The internal consistency of the instrument using Cronbach's alpha coefficient was 0.92. Finally, in order to perform the stability test, the Pearson correlation coefficient was calculated for all questions. This was above .05 and acceptable.Conclusions: FAFDQ can be used to make decisions, identify groups at risk of flood-related deaths, and implement flood-related death-reduction interventions. Indeed, these measures have led to the development of a comprehensive and reliable questionnaire for measuring the factors affecting flood deaths: a comprehensive set of factors that can be scientifically and accurately classified as flood-related deaths, appropriately categorizing the subjective and objective factors, psychometric assessment of the SFAFDQ, confirmatory factor analysis and questionnaire testing in a case-control Study.
Plasma proteomic profiling of pan-cancer patients discovers biomarkers of cancers
Authors: Bai Lin , Feng Jinwen , Qiao Xiaoqiang , Qu Yuanyuan , Lyu Jiacheng , Yang Guojian , Zhu Yuanxue , Gao Hui , Liao Lingxiao , Zang Aiming , Ding Wencong , Zhang Hailiang , Zhu Lingli , Wang Yan , Wang Liang , Wang Xiaofang , Li Yumiao , Li Jinghua , Yin Xiaoping , Zhao Guofa , Liu Dan , Gao Xiangpeng , Liao Yongshi , Ye Dingwei , Jia Youchao , Ding Chen 
Published: October 2021

Abstract
Circulatory system proteins play a central role in human physiology; therefore, profiling plasma proteins has been explored widely as a robust and dynamic tool in studying diseases. Here, we investigated the proteomic profile of plasma in 1,118 pan-cancer patients. The integrative analysis uncovered diverse molecular characteristics of different cancer types and bridged the plausible connection with the clinical features, including tumor stages. The findings demonstrate that the balance between lipid and glucose metabolisms is important in regulating immune infiltration. The pre- and post-surgery comparison of the plasma proteome indicated that tumor-induced angiogenesis is overexpressed in the tumor, and the differences between the proteome patterns could monitor post-surgery therapeutic effects. Finally, we developed a panel of tumor-type-specific proteins to classify tumor types with >95% sensitivity/specificity. Collectively, this study portrayed the plasma proteome landscape of human cancers and screened reliable biomarkers that could assist in diagnostic and drug discovery efforts.
One-year Risks and Burdens of Incident Cardiovascular Disease in COVID-19: Cardiovascular Manifestations of Long COVID
Authors: Al-Aly Ziyad , Bowe Benjamin , Xie Yan , Xu Evan 
Published: October 2021

Abstract
The cardiovascular complications of acute COVID-19 are well described; however, a comprehensive characterization of the post-acute cardiovascular manifestations of COVID-19 at one year has not been undertaken. Here we use the US Department of Veterans Affairs national healthcare databases to build a cohort of 151,195 people with COVID-19, 3,670,087 contemporary and 3,656,337 historical controls to estimate risks and 1-year burdens of a set of pre-specified incident cardiovascular outcomes. We show that beyond the first 30 days of infection, people with COVID-19 are at increased risk of incident cardiovascular disease spanning several categories including cerebrovascular disorders, dysrhythmias, ischemic and non-ischemic heart disease, pericarditis, myocarditis, heart failure, and thromboembolic disease. The risks and burdens were evident among those who were non-hospitalized during the acute phase of the infection and increased in a graded fashion according to care setting of the acute infection (non-hospitalized, hospitalized, and admitted to intensive care). Taken together, our results provide evidence that risk and 1-year burden of cardiovascular disease in survivors of acute COVID-19 are substantial. Care pathways of people who survived the acute episode of COVID-19 should include attention to cardiovascular health and disease.
Epithelial-Mesenchymal Transition-Related lncRNAs And SNAI2 Are Potential Biomarkers in Coronary Artery Disease
Authors: Xu Xiang , Zou Renchao , Liu Xiaoyong , Su Qianqian 
Published: October 2021

Abstract
BackgroundIncreasing evidence suggests that epithelial-mesenchymal transformation (EMT) is critical in the development of inflammatory response, atherosclerosis, and coronary artery disease (CAD). However, landscapes of EMT-related lncRNAs and their target genes have not been fully established in CAD.MethodsLncRNA and mRNA expression profiles obtained from Gene Expression Omnibus (GEO) database were used to identify the differentially expressed mRNAs (DEGs) and lncRNAs (DElncRNAs) between CAD and normal samples. Based on Pearson correlation analysis to identify the EMT-related lncRNAs, the optimal features were identified by receiver operating characteristic (ROC), the least absolute shrinkage and selection operator (LASSO) regression, Support Vector Machine Reverse Feature Elimination (SVM-RFE) algorithms, and logistic regression models were constructed aiming to distinguish CAD from normal samples. The cis and trans-regulatory networks were constructed based on EMT-related lncRNAs. We further estimated the infiltration of the immune cells in CAD patients with the CIBERSORT algorithm, and the correlation between key genes and infiltrating immune cells was analyzed.ResultsIn this study, a logistic regression model with powerful diagnostic capability was constructed based on a total of eight EMT-related lncRNAs identified by two machine learning methods. Then, results of the immune analysis revealed three significant immune cell subsets (CD8 T cells, monocytes, and NK cells) in CAD patients and found EMT-related lncRNAs were closely correlated with these immune cell subsets. By Pearson correlation analysis we got 34 “cis” and “trans” genes. Among them, SNAI2, an EMT-TF gene, was found in the trans-regulatory network of EMT-related lncRNAs. Further, through logistic regression and analysis of immune cell infiltration, we found SNAI2 was a potential biomarker for the diagnosis of CAD but also a close correlation between highly expressed SNAI2 and these three immune cell subsets in CAD patients.ConclusionIn conclusion, these biomarkers have important significance in the diagnosis of CAD patients. Eight EMT-related lncRNAs and SNAI2 can improve our understanding of the molecular mechanism between EMT and CAD.
Candidate Genes Underlying QTL for Powdery Mildew Resistance in Triticale (x Triticosecale Wittm.)
Authors: Dyda Mateusz Maksymilian , Tyrka Mirosław , Gołębiowska Gabriela , Rapacz Marcin , Wędzony Maria 
Published: October 2021

Abstract
Combining to tolerance to biotic and abiotic stresses is important target for modern triticale breeding. Cultivation of varieties resistant to fungal pathogens is economically and environmentally important and may lead to reducing of the use of fungicides. Molecular markers are necessary for accumulation of advantageous alleles in the best genotypes by means of marker-assisted and genomic selection approaches. In present research, QTL regions associated with the powdery mildew resistance at adult plant stage were evaluated in order to provide the effective selection tools. Testing of DH population in multiple environments under natural infestation revealed 20 QTL on wheat (4A, 3B, 4B) and rye (2R, 4R, 5R, 6R) chromosomes. Regions explained 8.1% - 29.3% of phenotypic variation depending of the trait, localization and year of the experiment. Main QTL with effect exceeding 15% were found on chromosomes 3B, 4B, 2R, 5R and 6R. QTL and candidate genes located on chromosomes 4B, 2R, 5R and 6R are so far reported for the first time as regions associated with PM resistance in the adult triticale plants. Additionally, within all QTL, 21 candidate genes associated with the PM resistance were revealed. Predicted function of protein encoded by these genes include triggering a defense system which restricts the pathogen growth, enzyme activity, regulation of hormone-activated pathways, transcriptional corepressor complex and cell wall construction. Availability of QTL, molecular markers together with candidate genes linked with the powdery mildew resistance can be validated on triticale lines and varieties and then, used in MAS to improve modern breeding.
Determinants and Reasons for Switching Anti-Retroviral Regimen Among HIV-Infected Youth in a Large Township of South Africa (2002-2019)
Authors: Kabarambi Anita , Balinda Sheila , Abaasa Andrew , Cogill Dolphina , Orrell Catherine 
Published: October 2021

Abstract
Background: There are limited data exploring antiretroviral therapy(ART) changes and time to change among South Africa young people living with HIV/AIDS. Objective: We describe the time to first drug switch, which includes ART regimen change (three drug switch) and substitutions (single drug switch). We describe common reasons for ART switch among young people aged 10 to 24 years in South Africa.Methods: We conducted a retrospective cohort study at a primary health care clinic in Cape Town, South Africa, providing ART to HIV-infected adolescents and adults since 2002. those aged 10 to 24 years at ART initiation, who accessed care clinic between September 2002 and April 2019. Data was retrieved from electronic information systems: ART regimens, ART changes, dates for initiation or stop of each drug/regimen, laboratory results (viral loads, haemoglobin, liver enzyme results, and creatinine to support the reason for ART switch. From written records, we abstracted reason for single drug switch or regimen change, as well as socio demographic and clinical data. We fitted cox regression models to determine factors associated with ART switch and the rate of occurrence. Results: Of 2601 adolescents included, 605 (24.9%) adolescents switched ART over 5090.5 person years at risk (PYAR), a rate of 11.9 /100PYAR. Median follow-up time was 4.4 (±3.2) years. At multivariable analysis, ART switch was independently associated with age 18-24 years, versus 10-17 years: adjusted Hazard Ratio [aHR] 0.78, 95% CI 0.62-0.98, transfer status [transferred out 1.42 [1.11-1.82]. The hazard of ART switch increased with more severe HIV-disease at ART start, as observed by increasing WHO clinical stage or reduced CD4 count at baseline. The primary reasons for ART switch were side effects (20.0%), virological failure (17.9%) and formulation switch (27.8%). Others reasons included pregnancy, Hepatitis B, tuberculosis and psychosis.Conclusion: ART switches are frequent and occur at a consistent rate across 7.5 years from initiation. The main reasons for ART switch were virological failure and drug side effects.
Cryptococcus gattii VGI Subtypes: Geographical Distribution, Molecular Traits, and Virulence Difference
Authors: Xue Xinying , Tang Wei , Zang Xuelei , Ke Weixin , Liu Yuxia , Li Zhiqiang , Xie Mei , Wang Lifeng , Shao Yiting , Wu Chongchong , Gao Jie , Ma Xidong , Zhao Sheng , Liu Pengfei , Zhang Qing , Deng Hui , Song Jialin , Deng Hengyu , Guo Shikun , Wang Xue , Huang Yemei , Gao Chengwen , Zhou Meng , Liu Sanhong , Xiao Meng , Wang Linqi , Shen Dingxia , Shi Yongyong , Zhang Liye 
Published: October 2021

Abstract
Background: As a life-threatening fungus, Cryptococcus gattii (C gattii) species complex is emerging worldwide. However, the geographical distribution, molecular traits, and virulence difference are poorly characterized in China.Results: From 2011 to 2017, we collected 32 strains of C gattii from 18 hospitals across China, of which 27 [84·4%] strains molecular traits were profiled by whole-genome sequencing (WGS) and multi-locus sequence typing (MLST) and compared with strains previously described in China from 2006 to 2020. Totally 119 clinical cases caused by C gattii strains (87 in previous reports and 32 in our study) distributed widely in 20 provincial-level administrative regions of China, of which 114 strains molecular types were obtained. The majority molecular type was VGI (81/114, 71·1%) and the other was VGII (33/114, 28·9%). Four major subtypes of VGI (VGIa, VGIb, VGIc, and VGId) were revealed from global C gattii VGI (n=308), respectively accounting for 52·9% (163/308), 36·0% (111/308), 3·9% (12/308), and 4·2% (13/308). The other nine strains could not be assigned to these four subtypes clearly. Our clinical data suggested that VGIb cases had a worse clinical outcome than VGIa, which was consistent with in vitro and in vivo experiments. In addition, a candidate virulence SNP on SOD2 in VGIa was initially identified by comparing high-quality de novo reference genome.Conclusions: The geographical distribution of C gattii species complex was first described in China. C gattii VGI could be clearly segregated into four major subtypes based on genomics profiles and VGIb was more virulent than VGIa in China. Our study suggests the molecular type of C gattii is necessary for personalized treatment in clinic.