bluuebunny/crossref_metadata_2025_split huggingface dataset:
Efficient removal of reactive blue-19 dye by co-electrospun nanofibers
Authors: Hakro Raheel Ahmed , Mehdi Mujahid , Qureshi Raja Fahad , Mahar Rasool Bux , Khatri Muzamil , Ahmed Farooq , Khatri Zeeshan , Kim Ick Soo 
Published: April 2021

Abstract
The present work demonstrates the new nanofiber mats prepared through co-electrospinning of two different polymers i.e. corn protein namely Zein and Nylon-6. The composite nanofiber membrane was used as an effective adsorbent material for the removal of toxic reactive dye i.e. Reactive Blue 19 (RB19) from water solution. These co-electrospun nanofibers had good mechanical strength compared to zein nanofibers alone. Experimental results suggested that zein/nylon nanofibers have greater potential for total removal of RB19 at room temperature within 10 min of contact time from aqueous solution. The maximum capacity was found to be 61.2 mg.g−1 of nanofibers. The mechanism of RB19 removal on proposed nanofibers is mainly through hydrogen bond and electrostatic means.
Quantum computer-aided design of quantum optics hardware
Authors: Kottmann Jakob S , Krenn Mario , Kyaw Thi Ha , Alperin-Lea Sumner , Aspuru-Guzik Alán 
Published: April 2021

Abstract
The parameters of a quantum system grow exponentially with the number of involved quantum particles. Hence, the associated memory requirement to store or manipulate the underlying wavefunction goes well beyond the limit of the best classical computers for quantum systems composed of a few dozen particles, leading to serious challenges in their numerical simulation. This implies that the verification and design of new quantum devices and experiments are fundamentally limited to small system size. It is not clear how the full potential of large quantum systems can be exploited. Here, we present the concept of quantum computer designed quantum hardware and apply it to the field of quantum optics. Specifically, we map complex experimental hardware for high-dimensional, many-body entangled photons into a gate-based quantum circuit. We show explicitly how digital quantum simulation of Boson sampling experiments can be realized. We then illustrate how to design quantum-optical setups for complex entangled photonic systems, such as high-dimensional Greenberger–Horne–Zeilinger states and their derivatives. Since photonic hardware is already on the edge of quantum supremacy and the development of gate-based quantum computers is rapidly advancing, our approach promises to be a useful tool for the future of quantum device design.
Rates of Undiagnosed Hypertension and Diagnosed Hypertension Without Anti-hypertensive Medication Following the Affordable Care Act
Authors: Huguet Nathalie , Larson Annie , Angier Heather , Marino Miguel , Green Beverly B , Moreno Laura , DeVoe Jennifer E 
Published: April 2021

Abstract

Background
The Affordable Care Act (ACA) Medicaid expansion improved access to health insurance and health care services. This study assessed whether the rate of patients with undiagnosed hypertension and the rate of patients with hypertension without anti-hypertensive medication decreased post-ACA in community health center (CHC).

Methods
We analyzed electronic health record data from 2012 to 2017 for 126,699 CHC patients aged 19–64 years with ≥1 visit pre-ACA and ≥1 post-ACA in 14 Medicaid expansion states. We estimated the prevalence of patients with undiagnosed hypertension (high blood pressure reading without a diagnosis for ≥1 day) and the prevalence of patients with hypertension without anti-hypertensive medication by year and health insurance type (continuously uninsured, continuously insured, gained insurance, and discontinuously insured). We compared the time to diagnosis or to anti-hypertensive medication pre- vs. post-ACA.

Results
Overall, 37.3% of patients had undiagnosed hypertension and 27.0% of patients with diagnosed hypertension were without a prescribed anti-hypertensive medication for ≥1 day during the study period. The rate of undiagnosed hypertension decreased from 2012 through 2017. Those who gained insurance had the lowest rates of undiagnosed hypertension (2012: 14.8%; 2017: 6.1%). Patients with hypertension were also more likely to receive anti-hypertension medication during this period, especially uninsured patients who experienced the largest decline (from 47.0% to 8.1%). Post-ACA, among patients with undiagnosed hypertension, time to diagnosis was shorter for those who gained insurance than other insurance types.

Conclusions
Those who gained health insurance were appropriately diagnosed with hypertension faster and more frequently post-ACA than those with other insurance types.

Clinical trials registration
Trial Number NCT03545763.
Loosening chromatin and dysregulated transcription: a perspective on cryptic transcription during mammalian aging
Authors: McCauley Brenna S , Dang Weiwei 
Published: April 2021

Abstract
Cryptic transcription, the initiation of transcription from non-promoter regions within a gene body, is a type of transcriptional dysregulation that occurs throughout eukaryotes. In mammals, cryptic transcription is normally repressed at the level of chromatin, and this process is increased upon perturbation of complexes that increase intragenic histone H3 lysine 4 methylation or decrease intragenic H3 lysine 36 methylation, DNA methylation, or nucleosome occupancy. Significantly, similar changes to chromatin structure occur during aging, and, indeed, recent work indicates that cryptic transcription is elevated during aging in mammalian stem cells. Although increased cryptic transcription is known to promote aging in yeast, whether elevated cryptic transcription also contributes to mammalian aging is unclear. There is ample evidence that perturbations known to increase cryptic transcription are deleterious in embryonic and adult stem cells, and in some cases phenocopy certain aging phenotypes. Furthermore, an increase in cryptic transcription requires or impedes pathways that are known to have reduced function during aging, potentially exacerbating other aging phenotypes. Thus, we propose that increased cryptic transcription contributes to mammalian stem cell aging.
TOP-Plus Is a Versatile Biosensor Platform for Monitoring SARS-CoV-2 Antibody Durability
Authors: Racine-Brzostek Sabrina E , Karbaschi Mohsen , Gaebler Christian , Klasse P J , Yee Jim , Caskey Marina , Yang He S , Hao Ying , Sukhu Ashley , Rand Sophie , Chadburn Amy , Shi Yuanyuan , Zuk Robert , Nussenzweig Michel C , Cushing Melissa M , Zhao Zhen 
Published: April 2021

Abstract

Background
Low initial severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antibody titers dropping to undetectable levels within months after infection have raised concerns about long-term immunity. Both the antibody levels and the avidity of the antibody–antigen interaction should be examined to understand the quality of the antibody response.

Methods
A testing-on-a-probe “plus” panel (TOP-Plus) was developed to include a newly developed avidity assay built into the previously described SARS-CoV-2 TOP assays that measured total antibody (TAb), surrogate neutralizing antibody (SNAb), IgM, and IgG on a versatile biosensor platform. TAb and SNAb levels were compared with avidity in previously infected individuals at 1.3 and 6.2 months after infection in paired samples from 80 patients with coronavirus disease 2019 (COVID-19). Sera from individuals vaccinated for SARS-CoV-2 were also evaluated for antibody avidity.

Results
The newly designed avidity assay in this TOP panel correlated well with a reference Bio-Layer Interferometry avidity assay (r = 0.88). The imprecision of the TOP avidity assay was &lt;10%. Although TAb and neutralization activity (by SNAb) decreased between 1.3 and 6.2 months after infection, the antibody avidity increased significantly (P &lt; 0.0001). Antibody avidity in 10 SARS-CoV-2 vaccinated individuals (median: 28 days after vaccination) was comparable to the measured antibody avidity in infected individuals (median: 26 days after infection).

Conclusions
This highly precise and versatile TOP-Plus panel with the ability to measure SARS-CoV-2 TAb, SNAb, IgG, and IgM antibody levels and avidity of individual sera on one sensor can become a valuable asset in monitoring not only patients infected with SARS-CoV-2 but also the status of individuals’ COVID-19 vaccination response.
Assortativity measures for weighted and directed networks
Authors: Yuan Yelie , Yan Jun , Zhang Panpan 
Published: April 2021

AbstractAssortativity measures the tendency of a vertex in a network being connected by other vertexes with respect to some vertex-specific features. Classical assortativity coefficients are defined for unweighted and undirected networks with respect to vertex degree. We propose a class of assortativity coefficients that capture the assortative characteristics and structure of weighted and directed networks more precisely. The vertex-to-vertex strength correlation is used as an example, but the proposed measure can be applied to any pair of vertex-specific features. The effectiveness of the proposed measure is assessed through extensive simulations based on prevalent random network models in comparison with existing assortativity measures. In application to World Input–Output Networks, the new measures reveal interesting insights that would not be obtained by using existing ones. An implementation is publicly available in a R package wdnet.
The benefits of being dominant: health correlates of male social rank and age in a marmot
Authors: Uchida Kenta , Ng Rachel , Vydro Samuel A , Smith Jennifer E , Blumstein Daniel T 
Published: April 2021

Abstract
The benefits of dominance may not come without costs, particularly for males. For example, the “immunocompetence handicap hypothesis” states that males with enhanced mating success allocate resources to enhance reproductive output at a cost to their current health, whereas the “resource quality hypothesis” predicts that high-ranking males may benefit from increased reproduction and good health. Whereas the predictions from each have been well tested in captive animals and in a variety of highly social primates, fewer studies have been carried out in free-living, facultatively social animals. Using adult male yellow-bellied marmots (Marmota flaviventer), we evaluated predictions of these hypotheses by examining the relationship between social rank and 2 health indicators—fecal glucocorticoid metabolite (FCM) levels, and neutrophil/lymphocyte (N/L) ratios—after accounting for variation explained by age, body mass, and seasonality. We found that higher-ranking males tended to have a lower N/L ratio (reflecting good health) than lower-ranking individuals, whereas FCM levels were not significantly related to rank. In addition, heavier male marmots had lower N/L ratios, whereas body mass was not associated with FCM levels. We also found that older adult males had lower FCM levels (reflecting less physiological stress) but higher N/L ratios than younger adults. Finally, we found that FCM levels decreased as the active season progressed and FCM levels were associated with the time of the day. Overall, our results suggest that socially-dominant male marmots enjoyed better, not worse health in terms of lower N/L ratios.
Gestational diabetes among women of migrant origin in Finland—a population-based study
Authors: Bastola Kalpana , Koponen Päivikki , Skogberg Natalia , Gissler Mika , Kinnunen Tarja I 
Published: April 2021

Abstract

Background
Migrant women may have a higher risk for gestational diabetes mellitus (GDM) and the related adverse outcomes. We studied the prevalence of GDM among migrant-origin women in Finland.

Methods
This study used data from the nationwide Medical Birth Register. Information on the most recent singleton births of women delivering between 2004 and 2014 (N = 379 634) was included. Women were classified into nine regional categories based on the country of origin. Finnish origin women were the reference group. Generalized linear models adjusted for maternal age, parity, socioeconomic position, pre-pregnancy body mass index and year of delivery were used to study the association between region/country of origin and GDM.

Results
Among the study population, almost 8% were of migrant origin. The prevalence of GDM varied from 6.1% (women of Latin American/Caribbean origin) to 18.4% (South Asian origin), compared to 8.7% in the Finnish reference group. When adjusted for confounders, women of South Asian, East Asian, Middle Eastern/North African and Russian/former USSR origin had a higher risk for GDM than Finnish origin women. By country of origin, women originating from Pakistan, Bangladesh, Sri Lanka, India, Afghanistan, Nepal, China, Philippines, Vietnam, Thailand, Morocco, Turkey, Iran, Iraq and former USSR had a higher risk for GDM than Finnish origin women.

Conclusions
There is substantial variation in the prevalence of GDM by country of origin. Women of South Asian, East Asian and Middle Eastern/North African origin had the highest risk for GDM and may warrant special attention.
Combining Different Line Crosses for Mapping Quantitative Trait Loci Using the Identical by Descent-Based Variance Component Method
Authors: Xie Chongqing , Gessler Damian D G , Xu Shizhong 
Published: April 2021

Abstract
Mapping quantitative trait loci (QTLs) is usually conducted with a single line cross. The power of such QTL mapping depends highly on the two parental lines. If the two lines are fixed for the same allele at a putative QTL, the QTL is undetectable. On the other hand, if a QTL is segregating in the line cross and is detected, the estimated variance of the QTL cannot be extrapolated beyond the statistical inference space of the two parental lines. To reduce the likelihood of missing a QTL and to increase the statistical inference space of the estimated QTL variance, we present a consensus QTL mapping strategy. We adopt the identical by descent (IBD)-based variance component method originally applied to human linkage analysis by combining multiple line crosses as independent families. We explore the properties of consensus QTL mapping and demonstrate the method with F2, backcross (BC), and full-sib (FS) families. In addition, we examine the effects of the QTL heritability, marker informativeness, QTL position, the number of families, and family size. We show that F2 families notably outperform BC and FS families in detecting a QTL. There is a substantial reduction in the standard deviation of the estimated QTL position and the separation of the QTL and polygenic variance. Finally, we show that the power to detect a QTL is greater when using a small number of large families than a large number of small families.
Coordination of Phytochrome Levels in phyB Mutants of Arabidopsis as Revealed by Apoprotein-Specific Monoclonal Antibodies
Authors: Hirschfeld Matthew , Tepperman James M , Clack Ted , Quail Peter H , Sharrock Robert A 
Published: April 2021

Abstract
Accumulating evidence indicates that individual members of the phytochrome family of photoreceptors have differential but interactive roles in controlling plant responses to light. To investigate possible cross-regulation of these receptors, we have identified monoclonal antibodies that specifically detect each of the five Arabidopsis phytochromes, phyA to phyE (phytochrome A holoprotein; PHYA, phytochrome A apoprotein; PHYA, phytochrome A gene; phyA, mutant allele of phytochrome A gene), on immunoblots and have used them to analyze the effects of phyA and phyB null mutations on the levels of all five family members. In phyB mutants, but not in phyA mutants, a four- to six-fold reduction in the level of phyC is observed in tissues grown either in the dark or in the light. Coordinate expression of phyB and phyC is induced in the phyB mutant background by the presence of a complementing PHYB transgene. However, in transgenic lines that overexpress phyB 15- to 20-fold, phyC is not similarly overexpressed. In these overexpressor lines, the levels of phyA, phyC, and phyD are increased two- to four-fold over normal in light-grown but not dark-grown seedlings. These observations indicate that molecular mechanisms for coordination or cross-regulation of phytochrome levels are active in Arabidopsis and have implications for the interpretation of phytochrome mutants and overexpressor lines.
The TERMINAL FLOWER2 (TFL2) Gene Controls the Reproductive Transition and Meristem Identity in Arabidopsis thaliana
Authors: Larsson Annika Sundås , Landberg Katarina , Meeks-Wagner D R 
Published: April 2021

Abstract
A new mutant of Arabidopsis thaliana that initiates flowering early and terminates the inflorescence with floral structures has been identified and named terminal flower2 (tfl2). While these phenotypes are similar to that of the terminal flower1 (tfl1) mutant, tfl2 mutant plants are also dwarfed in appearance, have reduced photoperiod sensitivity and have a more variable terminal flower structure. Under long-day and short-day growth conditions tfl1 tfl2 double mutants terminate the inflorescence without development of lateral flowers; thus, unlike tfl1 single mutants the double mutant inflorescence morphology is not affected by day length. The enhanced phenotype of the double mutant suggests that TFL2 acts in a developmental pathway distinct from TFL1. The complex nature of the tfl2 single mutant phenotype suggests that TFL2 has a regulatory role more global than that of TFL1. Double mutant analysis of tfl2 in combination with mutant alleles of the floral meristem identity genes LEAFY and APETALA1 demonstrates that TFL2 function influences developmental processes controlled by APETALA1, but not those regulated by LEAFY. Thus, the TFL2 gene product appears to have a dual role in regulating meristem activity, one being to regulate the meristem response to light signals affecting the development of the plant and the other being the maintenance of inflorescence meristem identity.
Molecular Organization of the 20S Proteasome Gene Family from Arabidopsis thaliana
Authors: Fu Hongyong , Doelling Jed H , Arendt Cassandra S , Hochstrasser Mark , Vierstra Richard D 
Published: April 2021

Abstract
The 20S proteasome is the proteolytic complex in eukaryotes responsible for degrading short-lived and abnormal intracellular proteins, especially those targeted by ubiquitin conjugation. The 700-kD complex exists as a hollow cylinder comprising four stacked rings with the catalytic sites located in the lumen. The two outer rings and the two inner rings are composed of seven different α and β polypeptides, respectively, giving an α7/β7/β7/α7 symmetric organization. Here we describe the molecular organization of the 20S proteasome from the plant Arabidopsis thaliana. From an analysis of a collection of cDNA and genomic clones, we identified a superfamily of 23 genes encoding all 14 of the Arabidopsis proteasome subunits, designated PAA-PAG and PBA-PBG for Proteasome Alpha and Beta subunits A–G, respectively. Four of the subunits likely are encoded by single genes, and the remaining subunits are encoded by families of at least 2 genes. Expression of the α and β subunit genes appears to be coordinately regulated. Three of the nine Arabidopsis proteasome subunit genes tested, PAC1 (α3), PAE1 (α5) and PBC2 (β3), could functionally replace their yeast orthologs, providing the first evidence for cross-species complementation of 20S subunit genes. Taken together, these results demonstrate that the 20S proteasome is structurally and functionally conserved among eukaryotes and suggest that the subunit arrangement of the Arabidopsis 20S proteasome is similar if not identical to that recently determined for the yeast complex.
Characterization of the Putative Transposase mRNA of Tag1, Which Is Ubiquitously Expressed in Arabidopsis and Can Be Induced by Agrobacterium-Mediated Transformation With dTag1 DNA
Authors: Liu Dong , Crawford Nigel M 
Published: April 2021

Abstract
Tag1 is an autonomous transposable element of Arabidopsis thaliana. Tag1 expression was examined in two ecotypes of Arabidopsis (Columbia and No-0) that were transformed with CaMV 35S-Tag1-GUS DNA. These ecotypes contain no endogenous Tag1 elements. A major 2.3-kb and several minor transcripts were detected in all major organs of the plants. The major transcript encoded a putative transposase of 84.2 kD with two nuclear localization signal sequences and a region conserved among transposases of the Ac or hAT family of elements. The abundance of Tag1 transcripts varied among transgenic lines and did not correlate with somatic excision frequency or germinal reversion rates, suggesting that factors other than transcript levels control Tag1 excision activity. In untransformed plants of the Landsberg ecotype, which contain two endogenous Tag1 elements, no Tag1 transcripts were detected. Agrobacterium-mediated transformation of these Landsberg plants with a defective 1.4-kb Tag1 element resulted in the appearance of full-length Tag1 transcripts from the endogenous elements. Transformation with control DNA containing no Tag1 sequences did not activate endogenous Tag1 expression. These results indicate that Agrobacterium-mediated transformation with dTag1 can activate the expression of Tag1.
Detection of Deleterious Genotypes in Multigenerational Studies. II. Theoretical and Experimental Dynamics with Selfing and Selection
Authors: Asmussen Marjorie A , Gilliland Laura U , Meagher Richard B 
Published: April 2021

Abstract
A mathematical model was developed to help interpret genotype and allele frequency dynamics in selfing populations, with or without apomixis. Our analysis provided explicit time-dependent solutions for the frequencies at diallelic loci in diploid populations under any combination of fertility, viability, and gametic selection through meiotic drive. With no outcrossing, allelic variation is always maintained under gametic selection alone, but with any fertility or viability differences, variation will ordinarily be maintained if and only if the net fitness (fertility × viability) of heterozygotes exceeds that of both homozygotes by a substantial margin. Under pure selfing and Mendelian segregation, heterozygotes must have a twofold fitness advantage; the level of overdominance necessary to preserve genetic diversity declines with apomixis, and increases with segregation distortion if this occurs equally and independently in male and female gametes. A case study was made of the Arabidopsis act2-1 actin mutant over multiple generations initiated from a heterozygous plant. The observed genotypic frequency dynamics were consistent with those predicted by our model for a deleterious, incompletely recessive mutant in either fertility or viability. The theoretical framework developed here should be very useful in dissecting the form(s) and strength of selection on diploid genotypes in populations with negligible levels of outcrossing.