bluuebunny/crossref_metadata_2025_split huggingface dataset:
Extracardiac conduit adequacy along the respiratory cycle in adolescent Fontan patients
Authors: Rijnberg Friso M , van der Woude Séline F S , Hazekamp Mark G , van den Boogaard Pieter J , Lamb Hildo J , Terol Espinosa de Los Monteros Covadonga , Kroft Lucia J M , Kenjeres Sasa , Karim Tawab , Jongbloed Monique R M , Westenberg Jos J M , Wentzel Jolanda J , Roest Arno A W 
Published: October 2021

Abstract

OBJECTIVES
Adequacy of 16–20mm extracardiac conduits for adolescent Fontan patients remains unknown. This study aims to evaluate conduit adequacy using the inferior vena cava (IVC)–conduit velocity mismatch factor along the respiratory cycle.

METHODS
Real-time 2D flow MRI was prospectively acquired in 50 extracardiac (16–20mm conduits) Fontan patients (mean age 16.9 ± 4.5 years) at the subhepatic IVC, conduit and superior vena cava. Hepatic venous flow was determined by subtracting IVC flow from conduit flow. The cross-sectional area (CSA) was reported for each vessel. Mean flow and velocity was calculated during the average respiratory cycle, inspiration and expiration. The IVC–conduit velocity mismatch factor was determined as follows: Vconduit/VIVC, where V is the mean velocity.

RESULTS
Median conduit CSA and IVC CSA were 221 mm2 (Q1–Q3 201–255) and 244 mm2 (Q1–Q3 203–265), respectively. From the IVC towards the conduit, flow rates increased significantly due to the entry of hepatic venous flow (IVC 1.9, Q1–Q3 1.5–2.2) versus conduit (3.3, Q1–Q3 2.5–4.0 l/min, P &lt; 0.001). Consequently, mean velocity significantly increased (IVC 12 (Q1–Q3 11–14 cm/s) versus conduit 25 (Q1–Q3 17–31 cm/s), P &lt; 0.001), resulting in a median IVC–conduit velocity mismatch of 1.8 (Q1–Q3 1.5–2.4), further augmenting during inspiration (median 2.3, Q1–Q3 1.8–3.0). IVC–conduit mismatch was inversely related to measured conduit size and positively correlated with conduit flow. The normalized IVC–conduit velocity mismatch factor during expiration and the entire respiratory cycle correlated with peak VO2 (r = –0.37, P = 0.014 and r = –0.31, P = 0.04, respectively).

CONCLUSIONS
Important blood flow accelerations are observed from the IVC towards the conduit in adolescent Fontan patients, which is related to peak VO2. This study, therefore, raises concerns that implanted 16–20mm conduits have become undersized for older Fontan patients and future studies should clarify its effect on long-term outcome.
Virtual exchange in teacher education: focus on L2 writing
Authors: Hilliker Shannon M , Yol Özge 
Published: October 2021

Abstract
Virtual exchange, as a global social practice, provides language teacher candidates with opportunities to connect their global peers and language learners. Integrated into teacher education, virtual exchange practices enhance teacher candidates’ professional and pedagogical knowledge and practices. This study details a virtual exchange program between TESOL teacher candidates in Poland and the US and examines the transformations in teacher candidates’ understanding and perspectives of theories and pedagogies of L2 writing. The findings of this single case indicate that virtual exchange is a strategy that can have productive outcomes that are worthy of further investigation as a means of broadening TESOL teachers’ knowledge and pedagogies of L2 writing.
Activity-induced boredom in online EFL classes
Authors: Derakhshan Ali , Kruk Mariusz , Mehdizadeh Mostafa , Pawlak Mirosław 
Published: October 2021

Abstract
The concept of boredom has recently attracted the attention of researchers in L2 education. As most of the available literature has focused on traditional, in-person classes, very little is known about how L2 students experience this aversive emotion in online classes, especially those prompted by the COVID-19 pandemic. To fill this gap, this large-scale, qualitative study surveys the opinion of 240 demographically versatile English major students from twenty-five universities in Iran to identify the activity types that are more likely to induce boredom in online classes. Thematic analysis of data, collected through open-ended questionnaires and semi-structured interviews and analysed through MAXQDA software, revealed ten different activity types which were boredom-inducing due to their non-engaging or cognitively (un)demanding nature. The findings are then discussed in light of relevant theories of boredom, and suggestions are given for making these activities less boring in online classes.
Standard care vs. TRIVEntricular pacing in Heart Failure (STRIVE HF): a prospective multicentre randomized controlled trial of triventricular pacing vs. conventional biventricular pacing in patients with heart failure and intermediate QRS left bundle branch block
Authors: Gould Justin , Claridge Simon , Jackson Thomas , Sieniewicz Benjamin J , Sidhu Baldeep S , Porter Bradley , Elliott Mark K , Mehta Vishal , Niederer Steven , Chadwick Humra , Kamdar Ravi , Adhya Shaumik , Patel Nikhil , Hamid Shoaib , Rogers Dominic , Nicolson William , Chan Cheuk F , Whinnett Zachary , Murgatroyd Francis , Lambiase Pier D , Rinaldi Christopher A 
Published: October 2021

Abstract

Aims
To determine whether triventricular (TriV) pacing is feasible and improves CRT response compared to conventional biventricular (BiV) pacing in patients with left bundle branch block (LBBB) and intermediate QRS prolongation (120–150 ms).

Methods and results
Between October 2015 and November 2019, 99 patients were recruited from 11 UK centres. Ninety-five patients were randomized 1:1 to receive TriV or BiV pacing systems. The primary endpoint was feasibility of TriV pacing. Secondary endpoints assessed symptomatic and remodelling response to CRT. Baseline characteristics were balanced between groups. In the TriV group, 43/46 (93.5%) patients underwent successful implantation vs. 47/49 (95.9%) in the BiV group. Feasibility of maintaining CRT at 6 months was similar in the TriV vs. BiV group (90.0% vs. 97.7%, P = 0.191). All-cause mortality was similar between TriV vs. BiV groups (4.3% vs. 8.2%, P = 0.678). There were no significant differences in echocardiographic LV volumes or clinical composite scores from baseline to 6-month follow-up between groups.

Conclusion
Implantation of two LV leads to deliver and maintain TriV pacing at 6 months is feasible without significant complications in the majority of patients. There was no evidence that TriV pacing improves CRT response or provides additional clinical benefit to patients with LBBB and intermediate QRS prolongation and cannot be recommended in this patient group.

Clinical trial registration number
Clinicaltrials.gov: NCT02529410.
Inductive foraging: patients taking the lead in diagnosis, a mixed-methods study
Authors: Michiels-Corsten Matthias , Weyand Anna M , Gold Judith , Bösner Stefan , Donner-Banzhoff Norbert 
Published: October 2021

Abstract

Background
Patient involvement in treatment decisions is widely accepted. Making a diagnosis, however, is still seen as a technical task mainly driven by physicians. Patients in this respect are perceived as passive providers of data. But, recent patient-centred concepts highlight the value of an active patient involvement in diagnosis.

Objective
We aim to reach a deeper understanding of how patients themselves contribute to the diagnostic process.

Methods
This is an observational study of patient consultations with their General Practitioner (GP) in 12 German practices. We performed a mixed-method qualitative and quantitative analysis of 134 primary care consultations.

Results
At the beginning of most consultations lies a phase where patients were invited to freely unfold their reason for encounter: This was named “inductive foraging” (IF). While patients actively present their complaints, GPs mainly listen and follow the presentation. This episode was found with every GP participating in this study. Ninety-one percent of consultations with diagnostic episodes were opened by IF. IF had a major contribution to the number of cues (diagnostic information) yielded in the diagnostic process. We illustrate a variety of tactics GPs make use of to invite, support, and terminate their patients in IF.

Conclusion
IF was found to be a highly relevant strategy in the diagnostic process. Patient involvement through IF offered a major contribution of diagnostic cues. We hypothesize that a patient-centred approach improves diagnosis.
The copper transporter, Ctr4, and the microtubule-associated protein, Cgp1, are important for Cryptococcus neoformans adaptation to nitrogen availability
Authors: Bosch Caylin , Volschenk Heinrich , Botha Alfred 
Published: October 2021

ABSTRACT
Nitrogen limitation was previously shown to be an important regulator of several genes associated with virulence in Cryptococcus neoformans. Among the most highly expressed genes under low-nitrogen conditions were CTR4 and CGP1, encoding a copper transporter and a microtubule-associated protein, respectively. However, the functional association of these genes with nitrogen limitation—a nutritional stress experienced in both environment and host—remains to be determined. Moreover, whether increased CTR4 and CGP1 expression is linked to the enhanced cryptococcal drug tolerance previously observed in low-nitrogen conditions is yet to be elucidated. Therefore, the present study explored the role of Cgp1 and Ctr4 in C. neoformans nitrogen stress adaptation and antifungal susceptibility. Our results showed that these genes play a role in the growth of C. neoformans in nitrogen-limited media, nitrogen source assimilation and growth on nitrogen-poor woody debris. Furthermore, we demonstrate that both Ctr4 and Cgp1 contribute to oxidative stress and antifungal susceptibility, with a ctr4∆ mutant being more susceptible to fluconazole and a cgp1∆ mutant being more susceptible to fluconazole and amphotericin B. Overall, our findings improve our understanding of the role of Ctr4 and Cgp1 in cryptococcal drug tolerance and adaptation to nitrogen availability.
Clinical characteristics and survival analysis of colorectal cancer in China: a retrospective cohort study with 13,328 patients from southern China
Authors: Fang Lekun , Yang Ziqing , Zhang Mingliang , Meng Manqi , Feng Junyan , Chen Chuangqi 
Published: October 2021

Abstract

Background
Colorectal cancer (CRC) is one of the most common cancers in China. However, detailed clinical characteristics and survival information are limited. This study aimed to investigate the potential epidemiological and clinical risk factors affecting the survival of CRC patients in southern China.

Methods
Patients with primary CRC between 1994 and 2019 at the First and the Sixth Affiliated Hospitals of Sun Yat-sen University (Guangzhou, China) were included. Clinical characteristics and survival outcomes were collected from medical records. The Kaplan–Meier method was used to estimate overall survival (OS) and progression-free survival (PFS), and Cox’s proportional-hazards regression model was used to estimate hazard ratios and 95% confidence intervals.

Results
Of all 13,328 patients, 60.1% were men; the mean age was 61.3 years; 53.5% had colon cancer. Among all patients, 1,864 (14.0%) were diagnosed with stage IV disease. The 3- and 5-year OS rates were 79.90% and 71.50%, respectively, whereas the 3- and 5-year PFS rates were 70.30% and 63.90%, respectively. The median OS and PFS times were 189 and 149 months, respectively. Among 13,328 patients, 428 (14.0%) patients with poor/undifferentiated cancer suffered recurrence. In patients with stage III and stage IV diseases, the median PFS times of the patients who received chemotherapy were significantly longer than those in patients who had not received chemotherapy (stage III: 147 vs 62 months, P &lt; 0.001; stage IV: 14 vs 9.5 months, P &lt; 0.001).

Conclusions
This retrospective cohort study illustrates the current status of the clinical characteristics of patients with CRC in southern China. Sex, age, family history, location of cancer occurrence, differentiation status, T status, N status, M status, clinical stage, operation, and surgical margin are independent factors associated with the OS of CRC patients.
Partner Pain and Affect in the Daily Lives of Older Couples
Authors: Potter Sophie , Röcke Christina , Gerstorf Denis , Brose Annette , Kolodziejczak Karolina , Hoppmann Christiane A , Ram Nilam , Drewelies Johanna 
Published: October 2021

AbstractObjectivesThe susceptibility of older adults’ affect to fluctuations in their own health (within-person health sensitivity) indicates how they handle everyday health challenges. In old age, affective well-being is often increasingly influenced by close others, yet it is unknown whether older adults’ affect is additionally susceptible to fluctuations in their spouse’s health (within-partnership health sensitivity) and the extent to which age and relationship satisfaction moderate such associations.MethodsParallel sets of multilevel actor–partner interdependence models are applied to self-reported health (feelings of pain/discomfort) and positive and negative affect, obtained 6 times a day over 7 consecutive days from 2 independent samples, the Berlin Couple Dynamics Study (N = 87 couples; Mage = 75 years; M relationship length = 46 years) and the Socio-Economic Panel Couple Dynamics Study (N = 151 couples; Mage = 72 years; M relationship length = 47 years).ResultsHusbands and wives had lower positive affect and higher negative affect in moments when they reported more pain (within-person health sensitivity) and when their respective spouse reported more pain (within-partnership health sensitivity). Tests for moderation suggest that within-person, but not within-partnership, health sensitivity is lower at older ages and higher with more satisfying relationships.DiscussionThese findings empirically illustrate life-span notions that close relationships shape time-varying health–affect links and thus underscore the theoretical and practical utility of examining social–contextual antecedents of older adults’ everyday affective well-being.
Social Networks and Cognitive Reserve: Network Structure Moderates the Association Between Amygdalar Volume and Cognitive Outcomes
Authors: Perry Brea L , Roth Adam R , Peng Siyun , Risacher Shannon L , Saykin Andrew J , Apostolova Liana G 
Published: October 2021

Abstract

Objectives
The cognitive reserve hypothesis has been proposed as a key mechanism explaining the link between social networks and cognitive function but has rarely been empirically tested using neuroimaging data. This study examines whether social network attributes moderate the association between amygdalar volume and cognitive function.

Methods
Data were from the Social Networks in Alzheimer Disease study (N = 154) and Indiana Alzheimer’s Disease Research Center. Social networks were measured using the PhenX Social Network Battery. Regional data from magnetic resonance imaging (amygdalar volume [AV]) were analyzed using FreeSurfer software. Cognitive function was measured using the Montreal Cognitive Assessment (MoCA) and consensus diagnosis. Linear regression analyses were conducted to test the moderating role of social networks on the association between AV and cognitive function.

Results
Participants with greater ability to span multiple social roles and subgroups within their networks scored higher on the MoCA after adjusting for sociodemographic variables, depression, frequency of contact, and AV. Social networks moderated the association between AV and cognitive function.

Discussion
Among participants who engaged in diverse and loosely connected social networks, the expected adverse cognitive effects of brain volume in regions implicated in socioemotional processing were attenuated. These findings suggest that cognitive stimulation achieved through social interaction with a diverse array of social relationships across multiple contexts may help promote cognitive reserve.
Interplay of large-scale tectonic deformation and local fluid injection investigated through seismicity patterns at the Reykjanes Geothermal Field, Iceland
Authors: Duboeuf Laure , Dichiarante Anna Maria , Oye Volker 
Published: October 2021

SUMMARY
Occurrence of seismicity sequences as consequence of fluid injection or extraction has long been studied and documented. Causal relations between injection parameters, such as injection pressure, injection rates, total injected volumes and injectivity, with seismicity derived parameters, such as seismicity rate, cumulative seismic moment, distance of seismicity (RT-plot), b-values, etc. have been derived. In addition, reservoir engineering parameters such as permeability/porosity relations and flow types play a role together with geology knowledge on fault and fracture properties, influenced by the stress field on different scales. In this paper, we study observed seismicity related to water injection at the Reykjanes Geothermal Field, Iceland. The region near the injection well did not experience seismicity before the start of injection. However, we observed continued seismic activity during the 3 months of injection in 2015, resulting in a cloud of about 700 events ranging in magnitude from Mw 0.7 to 3.3. We re-located these events using a modified double-difference algorithm and determined focal mechanism of event subsets. Characteristic for the site is that the events are bound to about 4 km distance to the injection point, and moreover known faults seem to act as barrier to fluids and seismicity. Several repeating sequences of seismicity, defined as bursts of seismicity have hypocenter migration velocities larger than 4 km d–1 and their dominant direction of propagation is away from the injection point towards larger depths. The seismic events within the bursts lack larger magnitude events, have elevated b-values (∼1.5) and consist of many multiplets. Except from the coinciding onset of seismicity with the start of fluid injection, no correlation between injection rates and volumes could be identified, neither could hydraulic diffusivity models explain observed seismicity patterns. Comparison of our results with investigations on background seismicity from 1995 to 2019 and from a seismic swarm in 1972 revealed similar focal mechanism patterns and burst-like seismicity patterns. We finally present a conceptual model where we propose that the observed seismicity patterns represent a stress release mechanism in the area close to the injection well, controlled by an interplay of local pore pressure and stress field changes with continued extensional stress build up at the Reykjanes Ridge.
Development of an exoglycosidase plate-based assay for detecting α1-3,4 fucosylation biomarker in individuals with HNF1A-MODY
Authors: Demus Daniel , Urbanowicz Paulina A , Gardner Richard A , Wu Haiyang , Juszczak Agata , Štambuk Tamara , Medvidović Edita Pape , Owen Katharine R , Gornik Olga , Juge Nathalie , Spencer Daniel I R 
Published: October 2021

Abstract
Maturity-onset diabetes of the young due to hepatocyte nuclear factor-1 alpha variants (HNF1A-MODY) causes monogenic diabetes. Individuals carrying damaging variants in HNF1A show decreased levels of α1-3,4 fucosylation, as demonstrated on antennary fucosylation of blood plasma N-glycans. The excellent diagnostic performance of this glycan biomarker in blood plasma N-glycans of individuals with HNF1A-MODY has been demonstrated using liquid chromatography methods. Here, we have developed a high-throughput exoglycosidase plate-based assay to measure α1-3,4 fucosylation levels in blood plasma samples. The assay has been optimized and its validity tested using 1000 clinical samples from a cohort of individuals with young-adult onset diabetes including cases with HNF1A-MODY. The α1-3,4 fucosylation levels in blood plasma showed a good differentiating power in identifying cases with damaging HNF1A variants, as demonstrated by receiver operating characteristic curve analysis with the AUC values of 0.87 and 0.95. This study supports future development of a simple diagnostic test to measure this glycan biomarker for application in a clinical setting.
N-acetyl-β-D-hexosaminidases mediate the generation of paucimannosidic proteins via a putative noncanonical truncation pathway in human neutrophils
Authors: Ugonotti Julian , Kawahara Rebeca , Loke Ian , Zhu Yuqi , Chatterjee Sayantani , Tjondro Harry C , Sumer-Bayraktar Zeynep , Neelamegham Sriram , Thaysen-Andersen Morten 
Published: October 2021

Abstract
We recently discovered that human neutrophils express immunomodulatory glycoproteins carrying unusual and highly truncated paucimannosidic N-glycans (Man1-3GlcNAc2Fuc0–1), but their biosynthesis remains elusive. Guided by the well-characterized truncation pathway in invertebrates and plants in which the N-acetyl-β-D-hexosaminidase (Hex) isoenzymes catalyze paucimannosidic protein (PMP) formation, we here set out to test if the homologous human Hex α and β subunits encoded by HEXA and HEXB drive a similar truncation pathway in human neutrophils. To this end, we performed quantitative glycomics and glycoproteomics of several CRISPR-Cas9-edited Hex-disrupted neutrophil-like HL-60 mutants (HEXA-KO and HEXB-KO) and matching unedited cell lines. Hex disruption was validated using next-generation sequencing, enzyme-linked immunosorbent assay (ELISA), quantitative proteomics and Hex activity assays. Excitingly, all Hex-disrupted mutants displayed significantly reduced levels of paucimannosylation, particularly Man2-3GlcNAc2Fuc1, relative to unedited HL-60 suggesting that both HEXA and HEXB contribute to PMP formation via a hitherto unexplored truncation pathway in neutrophils. Quantitative N-glycomics indeed demonstrated reduced utilization of a putative noncanonical truncation pathway in favor of the canonical elongation pathway in all Hex-disrupted mutants relative to unedited controls. Quantitative glycoproteomics recapitulated the truncation-to-elongation switch in all Hex-disrupted mutants and showed a greater switch for N-glycoproteins cotrafficking with Hex to the azurophilic granules of neutrophils such as myeloperoxidase. Finally, we supported the Hex-PMP relationship by documenting that primary neutrophils isolated from an early-onset Sandhoff disease patient (HEXB−/−) displayed dramatically reduced paucimannosylation relative to neutrophils from an age-matched unaffected donor. We conclude that both human Hex α and β mediate PMP formation via a putative noncanonical truncation pathway in neutrophils.