bluuebunny/crossref_metadata_2025_split huggingface dataset:
Green and Sustainable Preparation of Flower Like ZnO Nanostructures via Soft Bio-template Approach for the Enhancement of Biomedical Applications
Authors: Senthilkumar N. , Nandhakumar E. , Priya P. , Selvakumar M. , I Vetha Potheher 
Published: August 2021

Abstract
Investigation onthe biomedical applications of flower like Zinc Oxide (ZnO) nanostructures (NSs)synthesized by using aqueous extract of Oryza punctata (red rice) is reported for the first time. For the sustainable preparation of ZnO NSs, the precursors zinc nitrate and the rice extract act as the bio-template material. The powder X-ray diffraction analysis demonstrates the crystallographic structure and purity of the bio-prepared ZnO NSs. Fourier transform infrared spectral studies exhibits the existence of several functional groups in the rice extract and synthesized ZnO NSs. The FT-Raman spectroscopy analysis used to detect the presence of the defects in the synthesized material. The UV absorption onset shows the peaks at 270 nm & 370 nm for rice extract and ZnO NSs, respectively. The optimal energy bandgap value for the ZnO NSs was found to be 3.29 eV. The zinc oxygen vacancies, zinc interstitial and surface defects were determined by photoluminescence spectroscopy. The dynamic light scattering analysis reveals the material stability and average particle size distribution of the prepared ZnO NSs. The flower like morphology of the ZnO NSs was confirmed by field emission scanning electron microscopy analysis. ZnO NSs were tested for cytotoxicity activity against MCF–7 cell line and antibacterial properties using two gram positive (Staphylococcus Aureus, Bacillus Subtilis) and two gram negative bacteria (Salmonella Paratyphi, Escherichia Coli). Significant antioxidant activity was studied by the bio-prepared ZnO NSs against scavenging of DPPH (di(phenyl)-(2,4,6-trinitrophenyl)iminoazanium) free radicals. The flower like ZnO NSs showed a significant anti-arthritic activity which exhibited a maximum inhibition of protein denaturation (78.94±1.62 %) and membrane protective activity (81.12±1.25 %) at a dose of 0.5mg/mL concentration.
Timely and Appropriate Administration of Inhaled Argon Provides Better Outcomes for tMCAO Mice: a Controlled, Randomized and Double-blind Animal Study
Authors: He Juan , Xue Ke , Liu Jiayi , Gu Jin-hua , Peng Bin , Xu Lihua , Wang Guohua , Jiang Zhenglin , Li Xia , Zhang Yunfeng 
Published: August 2021

Abstract
Background: Inhaled argon (iAr) has shown promising therapeutic efficacy for acute ischemic stroke (AIS) and exhibited impressive advantages over other inert gases as a neuroprotective agent. However, the optimal dose, duration and time point of iAr for AIS are unknown. Here, we explored variable iAr schedules and evaluated the neuroprotective effects of acute iAr administration on lesion volume, brain edema, and neurological function in a mouse model of cerebral ischemic/reperfusion (I/R) injury.Methods: Adult ICR mice were randomly subjected to sham, moderate (1.5 h) or severe (3 h) transient middle cerebral artery occlusion (tMCAO). One hour after tMCAO, the mice were randomized to variable iAr protocols or air (iCtr). General and focal deficit scores were assessed during double-blind treatment. Infarct volume, overall recovery and brain edema were analyzed 24 h after cerebral I/R injury.Results: Compared with those in the tMCAO only group, lesion volume (p<0.001) and neurologic outcome (general, p<0.001; focal, p<0.001) were significantly improved in the iAr group, which was assigned to argon inhalation 1 h after ischemia (before the onset of reperfusion). Short-term argon treatment (1 h or 3 h) showed significantly better outcomes with regard to infarct volume (p<0.01) compared to argon inhalation for 24 h. The concentration of argon inhalation was confirmed to be a key factor in improving the focal neurological outcome relative to that in the tMCAO group, and higher concentrations showed better effects. In addition, even though ischemia research has shown an increase in cerebral damage proportional to ischemia time, argon administration showed significant neuroprotective efficacy on infarct volume (p<0.001), neurological deficits (general, p<0.001; focal, p<0.001), weight recovery (p<0.001), and edema (p<0.001) in general, particularly in moderate stroke.Conclusions: Timely argon inhalation before the onset of reperfusion showed optimal neurological outcomes and minimal infarct volumes. Moreover, an appropriate duration of argon administration was important for better neuroprotective efficacy. These findings may provide vital guidance for using argon as a neuroprotective agent and moving to clinical trials in acute ischemic stroke.
Consistent Role of Insulin Resistance in Recurrent Pregnancy Loss and Recurrent Implantation Failure: A Case-control Study
Authors: Ding Miao , He Fengyi , Li Xiaojia , Jiang Sushi , Cao Yacong , Zou Yanting , Yang Dongzi , Zhao Xiaomiao 
Published: August 2021

Abstract
Background: To explore the role of insulin resistance (IR) in patients with recurrent pregnancy loss (RPL) and/or recurrent implantation failure (RIF) treated with assisted reproductive technology (ART).Methods: We conducted a case-control study in a tertiary hospital from 2012 to 2018, We included 212 cases of simple RPL (only involved in RPL), 123 cases of simple RIF (only involved in RIF), 67 cases involved in both conditions (complicated group). We screened 123 women as the control cohort, who underwent ART due to male infertile, with no adverse pregnant outcomes. We examined the plasm glucose and insulin level in both fasting and postprandial condition after the oral glucose tolerance test (OGTT) and calculated the area under the curve of glucose (AUGG) and insulin (AUCI) as well as the homeostasis model assessment for insulin resistance and β-cell function (HOMA-IR and HOMA-β).Results: Both the simple RPL group and the complicated group had significantly higher fasting insulin (FINS), HOMA-IR and HOMA-β than the control group. The simple RIF group had the lowest level of FINS, HOMA-IR and HOMA-β. The incidences of IR were significantly higher in both the simple RPL group and the complicated group than the other two groups. After adjusted for age and waist-hip ratio (WHR), the simple RIF group had the highest fasting plasma glucose (FPG) [adjusted-mean (95%CI), 5.20 (5.09-5.33) mmol/L] and lower FINS [adjusted-mean (95%CI), 10.77 (9.25-12.29) mU/L] and HOMA-β [adjusted-mean (95%CI), 127.76 (83.56-171.97)]; the simple RPL group had the highest FINS [adjusted-mean (95%CI), 12.09 (11.21-12.98) mU/L] and HOMA-β [adjusted-mean (95%CI), 189.74 (164.29-215.18)] and a lower FPG [adjusted-mean (95%CI), 5.03 (4.97-5.10) mmol/L]. The FINS tended to increase with times of implantation failure among those patients with implantation failure fewer than six times. However, patients with more than six times implantation failure had extremely low FINS when compared with those with a history of five or six times (P<0.05). Conclusion: In patients undergoing ART, insulin resistance may be a common etiopathogenesis of RPL and RIF and insulin secretion impairment may be related to RIF.
Increased Plasma FGF21 and Activated FGF21 Signaling in Adipose Tissue and Its Possible Association With Insulin Sensitivity in Specific GDM Subtype
Authors: WANG Ning , SUN Bo , WANG Mengjun , MI Yang , CHEN Huan , SONG Lin , Cui Wei 
Published: August 2021

Abstract
Background: To study the discrepancy of the insulin sensitivity alteration pattern, circulating fibroblast growth factor (FGF21) levels and FGF21 signaling in visceral white adipose tissue (vWAT) of gestational diabetes mellitus (GDM) subtypes.Methods: 26 GDM women with either a predominant of insulin-secretion defect (GDM-dysfunction, n = 9) or insulin-sensitivity defect (GDM-resistance, n = 17)] and 13 normal glucose tolerance (NGT) women scheduled for caesarean-section at term were studied. Plasma and vWAT samples were collected at delivery.Results: The insulin sensitivity was improved from the 2nd trimester to delivery in the GDM-resistance group. Elevated circulating FGF21 concentration at delivery, increased FGF receptor 1c and decreased klotho beta gene expressions, enhanced ERK1/2 phosphorylation, and increased GLUT1, IR-B, PPAR-γ gene expressions in vWAT were found in the GDM-resistance group as compared with the NGT group. The circulating FGF21 concentration was negatively correlated with fasting blood glucose (r = -0.574, P < 0.001), and associated with the GDM-resistance group (r = 0.574, P < 0.001) in pregnant women at delivery. However, we observed no insulin sensitivity alteration in GDM-dysfunction and NGT groups during pregnancy. No differences of plasma FGF21 level and FGF21 signaling in vWAT at delivery were found between women in the GDM-dysfunction and the NGT group. Conclusions: Women with GDM heterogeneity exhibited different insulin sensitivity alteration patterns. The improvement of insulin sensitivity may relate to the elevated circulating FGF21 concentration and activated FGF21 signaling in vWAT at delivery in the GDM-resistance group.Trial registration: ChiCTR, ChiCTR 1900026735, registered 20 October 2019, https://www.chictr.org.cn/25/showproj.aspx?proj=44559
Identifying Parkinson’s disease subtypes with motor and non-motor symptoms via model-based multi-partition clustering
Authors: Rodriguez-Sanchez Fernando , Rodriguez-Blazquez Carmen , Bielza Concha , Larrañaga Pedro , Weintraub Daniel , Martinez-Martin Pablo , Rizos Alexandra , Schrag Anette , Chaudhuri K. Ray 
Published: August 2021

Abstract
Identification of Parkinson’s disease subtypes may help understand underlying disease mechanisms and provide personalized management. Although clustering methods have been previously used for subtyping, they have reported generic subtypes of limited relevance in real life practice because patients do not always fit into a single category. The aim of this study was to identify new subtypes assuming that patients could be grouped differently according to certain sets of related symptoms. To this purpose, a novel model-based multi-partition clustering method was applied on data from an international, multi-center, cross-sectional study of 402 Parkinson’s disease patients. Both motor and non-motor symptoms were considered. As a result, eight sets of related symptoms were identified. Each of them provided a different way to group patients: impulse control issues, overall non-motor symptoms, presence of dyskinesias and pyschosis, fatigue, axial symptoms and motor fluctuations, autonomic dysfunction, depression, and excessive sweating. Each of these groups could be seen as a subtype of the disease. Significant differences between subtypes (p < 0.01) were found in sex, age, age of onset, disease duration, and Hoehn & Yahr stage. Independent confirmation of these results could have implications for the clinical management of Parkinson’s disease patients.
Fungal endophytes of some medicinal plant growing in northwestern coast of Egypt: Isolation, identification and two-way clustering analysis of its antimicrobial activity
Authors: El-Metwally Mohammad Magdy , Eisa Atia Mohamed , Mekawey Amal AI , Mahmoud Samy F. , Halmouch Yasser El 
Published: August 2021

Abstract
One of hidden mine of antibiotics is endophytic fungi especially that inhibited medicinal plants. In this regard, leaves, stems, fruits and bulbs of some commonly medicinal plants growing in Northwestern coast of Egypt were subjected for isolation of endophytic fungi with screening study of its antimicrobial activity. Practically, more than one hundred (101) endophytic fungal species isolated from Scorpiurus muricatus, Mellilotus indicus, Lotus polyphyllos, Ononis vaginalis, Nicotiana glauca, Lycium europaeum, Asphodelus aestivus, Echium angustifolium,Fagonia cretica, Pancratium maritimum,and Carduus getulus were tested in vitro for their antimicrobial activities against E. coli, Pseudomonas argenosa, Staphylococcus aureus, Bacillus subtilus, Candida albicanus, Candida glabrata, Penicillium expansum, Aspergillus flavus. The values of applied diversity indices revealed significant differences in presence, absence and abundance among endophytic fungal isolates. To the best of our knowledge, the present study is the first to report of Alternaria pluriseptata as endophytic species with most dominant and most active in its anitimicrobial activity among the isolated species. Seven distinctive groups were revealed from the two-way cluster analysis showing the intensity of antimicrobial activity against tested pathogens: Twenty-five percent of the isolates (26 strains assembled together in group V) exhibited no antimicrobial activity against all tested pathogens while six percent (6 isolates) assembled in group VII revealed high antimicrobial activity against five pathogens.
The seven-layer stratification theory of gastric wall under ultrasonography and its clinical application
Authors: Mu Kexiao , Sun Qian 
Published: August 2021

Abstract
ObjectiveHere, we develop a seven-layer gastric wall stratification theory based on the physical basis of ultrasound and histology, and further discuss its potential clinical application. Methods1. Experimental methods: Ex vivo human gastric specimens were immersed in normal saline and examined with a high-frequency probe to study the relationship between the sonograms and the corresponding anatomy of the gastric wall. 2. The study enrolled 136 patients admitted to our hospital with gastric diseases who underwent gastric contrast ultrasonography supplemented with the pathological examination. The seven-layer stratification theory was adopted during the analysis to profile sonogram characteristics with lesions originating from various layers. ResultsAll the sonograms of the in vitro human gastric specimens could be divided into seven intervals of strong and weak echoes. The pathological examinations were performed on 136 patient-derived samples as the golden criteria of diagnosis: 29 cases of gastric polyps, 10 cases of lymphomas, 5 cases of neuroendocrine tumors, 11 cases of ectopic pancreas, 22 cases of gastric stromal tumor, 19 cases of leiomyomas, 29 cases of chronic inflammation, 9 cases of diffuse invasive cancer, and 2 cases of neurilemmoma. The ultrasound and pathological examination results were consistent in 110 cases, showing a coincidence rate of 80.9%. ConclusionBy adopting the seven-layer stratification theory of the gastric wall, the ultrasound can accurately locate the position of mucosal muscularis, which is of great significance for accurate measurement of the thickness of each anatomical layer and the correct judgment of the origin and the classification of the space-occupying lesions. Keywords Gastric wall; ultrasound; seven-layer stratification; clinical application
Perceiving SARS-CoV2 Mpro and PLpro dual inhibitors from pool of recognized antiviral compounds of endophytic microbes: an in-silico simulation study
Authors: Prajapati Jignesh , Patel Rohit , Rao Priyashi , Saraf Meenu , Rawal Rakesh , Goswami Dweipayan 
Published: August 2021

Abstract
The enormous impact of SARS-CoV2 continues and scientific community is seeking to discover the tactics to impede the spread of virus. The essential result is attenuated, and genetically engineered vaccines are being driven into the market with the general effectiveness being around 80%. Therefore, vaccination is not the sole answer for combat this pandemic. The substitute methodology is adapted to target on this virus with a medication in blend with existing vaccines. Papain like protease (nsp-3; nonstructural protein) and Mpro (nsp-5; nonstructural protein) of novel corona virus are the ideal target to develop drugs as they play different roles that are essential for viral development and replication. Utilizing computational methodology, we plan to distinguish a plausible microbial metabolite as analogue of GRL0617 (the well-established inhibitor of PLpro) and X77 (the well-established inhibitor of Mpro) from the pool of known antiviral compounds of endophytic microbes to interact and inhibit PLpro and Mpro as dual inhibitors. In the wake of collecting known antiviral compounds of endophytic microbes and screened them through pharmacophore hypothesis, molecular docking, and dynamics simulation, we perceive Cytonic acid A and Cytonic acid B to be seen as the potent PLpro and Mpro dual inhibitors using rigorous computational methods.
Identification of genomic regions associated with agronomical traits of bread wheat under two levels of salinity using GWAS
Authors: Alotaibi Fahad , Al-Qthanin Rahmah N. , Aljabri Maha , Albaqami Mohammed , Abou-Elwafa Salah 
Published: August 2021

Abstract
Soil salinity is a major environmental stress that adversely impacts the growth, development, productivity and quality of crop species, in particular, in arid and semi-arid regions. Identification of chromosomal regions associated with agronomic traits under salinity stress is crucial for improving salinity tolerance in wheat. GWAS and structure analyses were employed to evaluate 289 elite lines of the Wheat Association Mapping Initiative (WAMI) population under low (LS) and high (HS) salinity conditions using 15,737 SNP markers and seven agronomical traits. Evaluated genotypes responded differently to the different environments in all measured phenotypic traits, highlighting genetic diversity within the WAMI population in response to salt stress. Heritability degree ranged from moderate (37%) to high (88%). GWAS identified 118 and 120 significant associations between SNP markers and seven evaluated phenotypic traits under LS and HS conditions, respectively. Significant association of some markers with more than one phenotypic trait was observed, indicating possible pleiotropic or indirect effects. A high degree of significant linkage disequilibrium (> 52%) was observed among SNP markers on different chromosomes indicating epistatic interaction. The salt stress index (STI) exhibited a positive significant correlation to grain yield per plant (GYP) under both LS and HS conditions (R2 = 0.851–0.856). A linear regression between STI and GYP under HS conditions (GYPs) was identified, suggest that STI is the best tolerance index for predicting high yielding-genotypes. The results present the WAMI population as a valuable source for improving yield potential for salt tolerance in wheat. Furthermore, our findings emphasize that GWAS is a powerful tool in promoting wheat breeding through accurate identification of molecular markers significantly associated with agronomic traits, which is essential for marker-assisted breeding.
Ecological connectivity of seagrasses with mangroves increases the carbon storage of tropical seagrass meadows of an island ecosystem
Authors: Mishra Amrit Kumar , Apte Deepak , Farooq Syed Hilal 
Published: August 2021

Abstract
Ecologically connected ecosystems are considered more resilient to climate change mitigation by storing increased amounts of carbon than individual ecosystems. This study quantified the carbon storage capacity of seagrass (Thalassia hemprichii) meadows that are adjacent to mangroves (MG; Rhizophora apiculate) and without mangroves (WMG) at three locations in tropical Andaman and Nicobar Islands (ANI) of India. The sediment organic matter (OM) carbon (Corg) content was 2-fold higher at the MG sites than WMG sites of all three locations within the top 10 cm. The Corg in the total biomass was higher at MG sites than the biomass at WMG sites. The sediment grain size positively influenced the sediment OM and Corg content. The canopy height of T. hemprichii showed a better relationship with sediment OM and Corg at MG sites. In contrast, the shoot density of T. hemprichii showed a better relationship with sediment OM and Corg at WMG sites. The total carbon in 144 ha of T. hemprichii meadows of all three MG sites was 11031± 5223 Mg C, whereas the carbon in 148 ha of WMG sites was 4921±3725 Mg C. These T. hemprichii meadows of ANI store around 40487±19171 ton of CO2 in the MG sites and 18036 ±13672 ton of CO2 at WMG sites. The social cost of these carbon stored in these T. hemprichii meadows is around US$ 34.82 and 1.5 million at the MG and WMG sites, respectively. This study points out the efficiency of seagrass ecosystems of ANI as carbon sinks and the potential of these connected seascapes in increasing the efficiency of seagrass carbon storage. Therefore, this connectivity approach should be further explored to include these connected ecosystems of India as a nature-based solution for climate change mitigation and adaptation plans.
Peningkatan Kualitas Ruang dengan Intervensi Instalasi dari Material Barang Bekas di Hunian Mahasiswa Arsitektur dalam Mata Kuliah Studio Perancangan Dasar 02 Universitas Kristen Duta Wacana
Authors: Kristiadi Adimas , Sabono Ferdy 
Published: August 2021

Title: Improving Quality of Space with Intervention of The Installation of Used Materials in Architecture Student’s Residence, in Basic Design Studio 02 Duta Wacana Christian University

This research talks about the basic application of architectural design science with limited materials (response to the COVID-19 pandemic disaster). In this case, students are trained to see opportunities (looking for, identifying, processing, and using) used goods as design materials that can increase architectural usability. The Basic Design Studio (SPD) course builds basic skills of traditional craftsmanship, taste sensitivity, and insightful conceptual ideas. The estuary of the SPD course is to create a space installation to intervene in public spaces (physically) in people's lives with the special purpose of giving positive injections or stimulants to negative (dead) spaces so that they have attractiveness and at the same time can have a positive impact on the environment and society. The method of this research is deductive-qualitative, bringing the theory to see the case of how the intervention of negative space (dead) by SPD 02 students with the spatial installation design of used materials has an impact on people's lives. The output of this study is a standard room installation design from used materials that can improve the quality of negative space (dead) into positive space (living) in the residential and living environment around SPD 02 students, which does not rule out the possibility of being applied to a more spacious area. large.
Application of dynamic and conceptual models for simulating flow hydrographs in an urbanized catchment under conditions of controlled outflow from stormwater tanks
Authors: Barszcz Mariusz P. 
Published: August 2021

Abstract
The main aim of the research presented in the work was to assess the usefulness of the dynamic SWMM (stormwater management model) and the conceptual SBUH (Santa Barbara Urban Hydrograph) model for simulating and predicting flow hydrographs in a small urbanized catchment under conditions of controlled (using valves) outflow from stormwater retention tanks in response to rainfall events. Most of the analyzed catchment of the Służewiecki Stream consists of the area beneath F. Chopin International Airport in Warsaw. A further aim of the study was the development of method for indicating the concentration time for a given rainfall–runoff event, where the influence of delaying the outflow of stormwater from the catchment as a result of its retention in tanks on the value of this parameter will be accounted for. The values of the median of absolute errors, obtained in a simulation using the SWMM in relation to peak flows and hydrograph volumes for the analyzed events, were 15.4 and 18.4%, respectively. The adequate values of simulation errors, obtained in the SBUH model using concentration times determined according to the developed method, were 11.4 and 15.4%. Satisfactory results of simulations were received using both models.