bluuebunny/crossref_metadata_2025_split huggingface dataset:
Reovirus infection is regulated by NPC1 and endosomal cholesterol homeostasis
Authors: Ortega-Gonzalez Paula , Taylor Gwen , Jangra Rohit K. , Tenorio Raquel , de Castro Isabel Fernández , Mainou Bernardo A. , Orchard Robert C. , Wilen Craig B. , Brigleb Pamela H. , Sojati Jorna , Chandran Kartik , Risco Cristina , Dermody Terence S. 
Published: September 2021

AbstractCholesterol homeostasis is required for the replication of many viruses, including Ebola virus, hepatitis C virus, and human immunodeficiency virus-1. Niemann-Pick C1 (NPC1) is an endosomal-lysosomal membrane protein involved in cholesterol trafficking from late endosomes and lysosomes to the endoplasmic reticulum. We identified NPC1 in CRISPR and RNA interference screens as a putative host factor for infection by mammalian orthoreovirus (reovirus). Following internalization via clathrin-mediated endocytosis, the reovirus outer capsid is proteolytically removed, the endosomal membrane is disrupted, and the viral core is released into the cytoplasm where viral transcription, genome replication, and assembly take place. We found that reovirus infection is significantly impaired in cells lacking NPC1, but infection is restored by treatment of cells with hydroxypropyl-β-cyclodextrin, which binds and solubilizes cholesterol. Absence of NPC1 did not dampen infection by infectious subvirion particles, which are reovirus disassembly intermediates that bypass the endocytic pathway for infection of target cells. NPC1 is not required for reovirus attachment to the plasma membrane, internalization into cells, or uncoating within endosomes. Instead, NPC1 is required for delivery of transcriptionally active reovirus core particles into the cytoplasm. These findings suggest that cholesterol homeostasis, ensured by NPC1 transport activity, is required for reovirus penetration into the cytoplasm, pointing to a new function for NPC1 and cholesterol homeostasis in viral infection.Author summaryGenetic screens are useful strategies to identify host factors required for viral infection. NPC1 was identified in independent CRISPR and RNA interference screens as a putative host factor required for reovirus replication. We discovered that NPC1-mediated cholesterol transport is dispensable for reovirus attachment, internalization, and disassembly but required for penetration of the viral disassembly intermediate from late endosomes into the cytoplasm. These findings pinpoint an essential function for cholesterol in the entry of reovirus and raise the possibility that cholesterol homeostasis regulates the entry of other viruses that penetrate late endosomes to initiate replication.
The APC/CCCS52A2 E3-Ligase Complex targeted PKN1 Protein is a Plant Lineage-Specific Driver of Cell Division
Authors: Willems Alex , Liang Yuanke , Heyman Jefri , Eekhout Thomas , Van den Daele Hilde , Vercauteren Ilse , De Veylder Lieven 
Published: September 2021

ABSTRACTThe anaphase-promoting complex/cyclosome (APC/C) marks key cell cycle proteins for proteasomal breakdown, thereby ensuring unidirectional progression through the cell cycle. Its target recognition is temporally regulated by activating subunits, one of which is called CELL CYCLE SWITCH 52 A2 (CCS52A2). We sought to expand the knowledge of identified APC/C targets by using the severe growth phenotypes of CCS52A2-deficient Arabidopsis thaliana plants as a readout in a suppressor mutagenesis screen, resulting in the identification of the previously undescribed gene called PIKMIN1 (PKN1). PKN1 deficiency rescues the disorganized root stem cell phenotype of the ccs52a2-1 mutant, whereas an excess of PKN1 inhibits growth of ccs52a2-1 plants, indicating the importance of PKN1 abundance for proper development. Accordingly, the lack of PKN1 in a wild-type background negatively impacts cell division, while its ectopic expression promotes proliferation. PKN1 shows a cell cycle phase-dependent accumulation pattern, localizing to microtubular structures, including the preprophase band, the mitotic spindle, and phragmoplast. PKN1 is conserved throughout the plant kingdom, with its function in cell division being evolutionary conserved in the liverwort Marchantia polymorpha. Our data thus demonstrate that PKN1 represents a novel, plant-specific gene with a rate-limiting role in cell division, which is proteolytically controlled by the CCS52A2-activated APC/C.One-Sentence SummaryPKN1 is a conserved plant-specific protein that is rate-limiting for cell division, likely through its interaction with microtubuli, and is proteolytically controlled by APC/CCCS52A2.
Dynein acts to cluster glutamate receptors and traffic the PIP5 kinase, Skittles, to regulate postsynaptic membrane organization at the neuromuscular junction
Authors: Neisch Amanda L. , Pengo Thomas , Avery Adam W. , Li Min-Gang , Hays Thomas S. 
Published: September 2021

AbstractCytoplasmic dynein is essential in motoneurons for retrograde cargo transport that sustains neuronal connectivity. Little, however, is known about dynein’s function on the postsynaptic side of the circuit. Here we report distinct postsynaptic roles for dynein at neuromuscular junctions (NMJs). Intriguingly, we show that dynein punctae accumulate postsynaptically at glutamatergic synaptic terminals. Moreover, Skittles, a phosphatidylinositol 4-phosphate 5-kinase that produces PI(4,5)P2 to organize the spectrin cytoskeleton, also localizes specifically to glutamatergic synaptic terminals. Depletion of postsynaptic dynein disrupts the accumulation of Skittles, PI(4,5)P2 phospholipid, and organization of the spectrin cytoskeleton at the postsynaptic membrane. Coincidental with dynein depletion, we observe an increase in the clusters size of ionotropic glutamate receptor (iGluR), and an increase in the amplitude and frequency of mEJPs. However, PI(4,5)P2 levels do not affect iGluR clustering and dynein does not affect the protein levels of iGluR subunits at the NMJ, suggesting a separate, transport independent function for dynein in iGluR cluster organization. As dynein punctae closely associate with iGluR clusters, we propose that dynein physically tethers iGluR clusters at the postsynaptic membrane to ensure proper synaptic transmission.
Leukocyte adhesion is governed by endolysosomal two pore channel 2 (TPC2)
Authors: Goretzko Jonas , Heitzig Nicole , Thomas Katharina , Krogsaeter Einar Kleinhans , Naß Johannes , Linard Matos Anna Lívia , Wegner Tristan , Schloer Sebastian , Gerke Volker , Rossaint Jan , Glorius Frank , Bracher Franz , Grimm Christian , Rescher Ursula 
Published: September 2021

AbstractIn response to pro-inflammatory challenges including pathogenic attack and tissue damage, the endothelial cell surface is rearranged to present leukocyte-engaging cell surface receptors. The initial contact needed for leukocyte tethering and rolling is mediated via adhesion demand-driven exocytosis of Weibel-Palade bodies (WPB) that contain the leukocyte receptor P-selectin together with the stabilizing co-factor CD63. We found that diminished expression of the endolysosomal non-selective cation channel TPC2 or inhibition of TPC2-mediated Ca2+-release via trans-Ned 19 led to reduced endolysosomal Ca2+ efflux, and blocked transfer of CD63 from late endosomes/lysosomes (LEL) to WPB, and a concomitant loss of P-selectin on the endothelial cell surface. Accordingly, P-selectin-mediated leukocyte recruitment to trans-Ned 19-treated HUVEC under flow was significantly reduced without disturbing VWF exocytosis. Our findings establish the endolysosome-related TPC2 Ca2+ channel as a key element in the maintenance of proper endothelial functions and a potential pharmacological target in the control of inflammatory leukocyte recruitment.
The role of social media in food waste prevention behaviour
Authors: Teoh Chai Wen , Koay Kian Yeik , Chai Pei Sun 
Published: September 2021

PurposeThis research aims to investigate consumers' food waste prevention behaviour through the lens of an extended theory of planned behaviour. The extension includes the examination of the role of social media usage in affecting consumers' decision-making process of food waste prevention behaviour.Design/methodology/approachA survey questionnaire is developed based on measurement items of previously validated studies. 210 samples are collected and analysed using partial least squares structural equation modelling (PLS-SEM).FindingsThe results show that social media usage has a significant influence on attitude and injunctive norms. In addition, intention can be predicted by injunctive norms, moral norms and perceived behavioural control but not attitude. Lastly, the intention to reduce food waste is a significant predictor of food waste prevention behaviour.Originality/valueOverall, the extended theory of planned behaviour is useful in explaining consumers' food waste prevention behaviour. Interestingly, this study reveals that a negative attitude towards food waste behaviour does not translate into the intention to engage in food waste prevention behaviour. Furthermore, it is found that social media usage plays a significant in shaping consumers' attitudes towards food waste and injunctive norms.
Numerical simulation of the electroosmotic flow of the Carreau-Yasuda model in the rectangular microchannel
Authors: Ghorbani Saeed , Jabari Moghadam Ali , Emamian Amin , Ellahi R. , Sait Sadiq M. 
Published: September 2021

Purpose
In this paper aims to investigate the numerical simulation of the electroosmotic flow of the Carreau-Yasuda model in the rectangular microchannel. Electromagnetic current is generated by applying an effective electric field in the direction of the current.

Design/methodology/approach
The non-Newtonian model used is the five-constant Carreau-Yasuda model which the non-Newtonian properties of the fluid can be well modeled. Using the finite difference method, the potential values at all points in the domain are obtained. Then, the governing equations (momentum conservation) and the energy equation are segregated and solved using a finite difference method.

Findings
In this paper, the effect of various parameters such as Weisenberg number, electrokinetic diameter, exponential power number on the velocity field and Brinkman and Pecklet dimensionless numbers on temperature distribution are investigated. The results show that increasing the Weissenberg dimensionless number and exponential power and diameter parameters reduces the maximum velocity field in the microchannel.

Originality/value
To the best of the authors’ knowledge, this study is reported for the first time.
Bansho as part of lesson and lesson study: from the origins to the present
Authors: Tan Shirley 
Published: September 2021

PurposeThis paper aims to provide an overview of the origins of bansho, how it has developed to be an indispensable practice in Japan and its influence on countries outside Japan. The integration of bansho into lesson study (LS) will also be presented.Design/methodology/approachThis paper draws on the vast literature on bansho from both in and outside Japan to illustrate the points mentioned above.FindingsRecapitulation on the history of chalkboard and bansho in different eras in Japan illustrated its endurance towards the test of time, geographical space and functions. While bansho remains a constant presence in LS research, there has not been any work that outlines how bansho is integrated into the LS cycle. Thus, a guideline on how bansho can be incorporated in the LS cycle is provided.Originality/valueA comprehensive overview of bansho allows educators and researchers outside Japan to move beyond the know-how and know-what of bansho; it would serve as an invaluable source to understand the core concepts underlying this classroom practice, thus avoiding the mere adaptation of bansho superficial aspects. This study also provides precise instruction on how bansho can be integrated into each step of the LS cycle, which will guide teachers and researchers in the future implementation of LS.
Software platform owners' entry and application developers' innovations: a conceptual model
Authors: Zuo Yan , Song Di 
Published: September 2021

PurposeThe primary aim of this article is to develop an understanding that resolves and integrates the conflicting findings with regard to the effects of platform-owner entry on the innovation of individual complementors.Design/methodology/approachDrawing on the platform ecosystem literature and the profiting from innovation (PFI) framework, this study presents a conceptual model that articulates how developers' marketing capabilities and the size of platform's installed base are two key moderators that explain the conflicting results between platform-owner entry and complementor innovations.FindingsThis article theorizes that platform owners' entry stimulates developers' innovations when the size of platform's installed base is large or when developers' marketing capabilities are strong while the entry can discourage innovations otherwise.Originality/valueBy proposing the conceptual model, this article makes important theoretical contributions to the rising literature on platform governance and complementor innovations. It lays a foundation for future research exploring the implications of platform-owner entry.
The factors influencing technological entrepreneurship in nanotechnology businesses
Authors: Nikraftar Tayebeh , Hosseini Elahe , Mohammadi Elham 
Published: September 2021

PurposeTechnological entrepreneurship has been a very significant topic in recent decades. It has a crucial role in economic modernization and growth. The need for technological entrepreneurship is because technology-based industries are expanding rapidly and are replacing traditional industries. Therefore, this study aimed at identifying the factors affecting the success of technological entrepreneurship in Iranian nanotechnology businesses.Design/methodology/approachThe research was conducted through mixed method. The participants in the qualitative section included 17 university experts and executive managers in the field of nanotechnology in Iran, and 75 nanotechnology business managers participated in the quantitative section. The interview and questionnaire were used to collect information. In order to measure and fit the models, the confirmatory factor analysis method and PLS3 software were used.FindingsThe results indicated that the key factors affecting the success of the technological entrepreneurship process in nanotechnology were classified into five general categories: organizational, environmental, institutional, individual and technology factors. Moreover, it was shown that all these dimensions had a positive and significant effect on technological entrepreneurship. In addition, the organizational dimension has an essential role.Originality/valueCompanies' ability to engage technological entrepreneurship is a vital factor in human resource management and strategic management. However, technological entrepreneurship in Iranian nanotechnology businesses has not been involved integrally in the context of companies.
Functional diversity of macroinvertebrates as a tool to evaluate wetland restoration
Authors: Coccia Cristina , Almeida Bia A. , Green Andy J. , Gutiérrez Ana Belén , Carbonell José Antonio 
Published: September 2021

Abstract

Ecological restoration of aquatic ecosystems has become widespread in recent decades. Although the recovery of biodiversity in restored wetlands has been studied from a taxonomic perspective, our knowledge of how functional biodiversity recovers remains poorly understood.

We studied the functional diversity of macroinvertebrate communities in 32 Mediterranean temporary ponds 6–7 years after their creation during a restoration in South‐West Spain, and compared them with 10 natural reference sites during two consecutive hydroperiods. We compared alpha functional diversity indices, and the individual contributions of new ponds and reference sites to the regional functional beta diversity, as well as to its turnover and nestedness components. We also investigated the influence of environmental and spatial variables on the dissimilarities of functional beta diversity and its components between new ponds and reference sites.

Alpha functional diversity in new ponds was lower than in reference sites. Although the contribution of new ponds to the regional functional beta diversity was similar to that of reference sites, the latter contributed more to functional turnover while new ponds contributed more to functional nestedness.

Dispersal limitation coupled with environmental filtering structured the functional variation in communities between new ponds and reference sites, but their relative importance differed between beta components. New ponds can hold species with unique functional compositions, but their contribution to the regional functional beta diversity was mostly due to trait losses with respect to reference sites.

Synthesis and applications. Considering different aspects of functional diversity of invertebrate communities can help elucidate the processes and mechanisms through which ecosystems recover following restoration. We encourage the use of trait‐based approaches to identify trends in processes and patterns that can guide future wetland restoration projects.
Variation in biomass allocation and root functional parameters in response to fire history in Brazilian savannas
Authors: Le Stradic Soizig , Roumet Catherine , Durigan Giselda , Cancian Leonardo , Fidelis Alessandra 
Published: September 2021

Abstract

Fire is a fundamental ecological factor in savannas because it affects vegetation dynamics and ecosystem functioning. However, the effects of fire on below‐ground compartments, including biomass and root traits, and their regeneration remain poorly understood. In this study, we assess the variation of above‐ and below‐ground plant components along fire‐history gradients in Brazilian open savannas and investigate whether changes in vegetation and soil properties are associated with the responses of below‐ground biomass and root traits.

The study was conducted in eight sampling areas of open savanna (campo sujo, i.e. vegetation having low woody cover) within the Cerrado (Brazilian savannas), located along a gradient of time since the last fire (1–34 years); the number of fires that occurred within the past 34 years (0–9 fires) varied by sampling area. In each sampling area, we measured above‐ and below‐ground biomass, root depth distribution, root functional parameters and nutrient levels in the upper soil layers (0–10 cm).

Rapid recovery of above‐ground live biomass after a fire was primarily due to resprouting of graminoids. This recovery was associated with an increase in absorptive root biomass in the upper soil layer in the most recently burnt sites, whereas root biomass was unaffected in deeper layers. Root parameters remained constant regardless of fire history but responded to variations in vegetation structure and soil properties. Specific root length (SRL) decreased with K, Mg2+, Al3+, N and C and increased with P concentration. In contrast, root tissue density (RTD) and absorptive root proportion were negatively correlated with soil P. RTD was strongly associated with the above‐ground biomass of graminoids. Soil texture impacted the root system: the proportion of absorptive roots increased with fine sand content in the soil, inversely to transport root biomass. The relationship between fire and soil properties was insignificant.

Synthesis. In savannas, fire stimulates absorptive root biomass in response to the higher demand for below‐ground resources. This response is correlated with shoot regrowth after a fire. Variations in morphological root parameters are not directly associated with fire history; instead, they reflect differences in soil chemistry, especially soil P and graminoid biomass changes.
Escalated grades of complications correlate with incremental costs of video‐assisted thoracoscopic surgery major lung resection for lung cancer in China
Authors: Lan Ke , Zhou Jian , Sui Xizhao , Wang Jun , Yang Fan 
Published: September 2021

AbstractObjectiveFew studies have focused on factors associated with the incremental cost of video‐assisted thoracoscopic surgery (VATS) in China. We aim to systematically classify the complications after VATS major lung resection and explore their correlation with hospital costs.MethodsPatients with pathologically stage I–III lung cancer who underwent VATS major lung resections from January 2007 to December 2018 were included. The Thoracic Mortality and Morbidity (TM&M) Classification system was used to evaluate postoperative complications. Grade I and II complications, defined as minor complications, require no therapy or pharmacologic intervention only. Grade III and IV complications, defined as major complications, require surgical intervention or life support. Grade V results in death. A generalized linear model was used to explore the correlation of incremental hospital costs and complications, as well as other clinicopathologic parameters between 2013 and 2016.ResultsA total of 2881 patients were enrolled in the first part, and the minor and major complications rates were 24.3% (703 patients) and 8.3% (228 patients), respectively. Six hundred and eighty‐two patients were enrolled in the second part. The complications grade II (odds ratio [OR] 1.12, 95% confidence interval [CI] 1.05–1.2, p = 0.0005), grade III (OR 1.55, 95% CI 1.26–1.9, p < 0.0001), grades IV and V (OR 1.09, 95% CI 1.04–1.13, p = 0.0002), diffusion capacity of carbon dioxide (OR 0.998, 95% CI 0.997–1.000, p = 0.004), and duration of chest drainage (OR 1.03, 95% CI 1.02–1.04, p < 0.001) and were independent risk factors for the increase in in‐hospital costs of VATS major lung resections.ConclusionsThe severity of complications graded by the TM&M system was an independent risk factor for increased in‐hospital costs.
Positive tone and initial coin offering
Authors: Zhang Shuyu , Aerts Walter , Zhang Dunli , Chen Zishan 
Published: September 2021

AbstractAn initial coin offering (ICO) has become a popular venue for crowdfunding at an early stage in a blockchain project. In the ICO process, a whitepaper may serve as an effective marketing tool to influence investor perception and investor trading behaviour. In this paper, we investigate whether management sentiment, as captured by the disclosure tone of the ICO whitepaper, is seen as a credible signal and capable of affecting price behaviour of the ICO on its first trading day. We document a positive association between management’s net positive tone in the whitepaper and ICO first‐day return, indicating that management sentiment has a significant impact on investor behaviour. Our findings show, however, that such association is contingent on the presence of causal argument, suggesting that disclosure tone needs a significant extent of causal reasoning support to back up its credibility and make it persuasive. These findings provide incremental evidence on the relationship between management tone and relative asset returns during an ICO process in the cryptocurrency markets. While management may benefit from soft information disclosure in the whitepaper, investors and regulators should be aware of self‐serving incentives in whitepaper disclosures.