bluuebunny/crossref_metadata_2025_split huggingface dataset:
Reduced IL-37 gene expression and CD8 T lymphocytes in patients with metastatic breast cancer
Authors: Farahani Najmeh , Mohagheghi Fathollah , Mosayebi Ghasem , Ghazavi Ali , Ganji Ali 
Published: May 2021

BACKGROUND: The exact immunopathological mechanisms in the progression of breast cancer are not clearly understood, but various factors including CD8 T lymphocytes have lethal properties on tumor cells. On the other hand, interleukin-37 (IL-37), as a new member of the IL-1 family, is an anti-inflammatory cytokine. The exact role of IL-37 in breast cancer has not yet been determined. OBJECTIVE: This study aimed to evaluate the CD8 T lymphocytes count and IL-37 gene expression in newly diagnosed breast cancer patients with and without metastasis. METHODS: In this study, blood samples from 36 metastatic and 36 non-metastatic breast cancer patients and 36 healthy individuals as control were collected. After RNA extraction and cDNA synthesis, the relative gene expression was performed using real-time PCR. Also, counting the CD8 T lymphocytes was done by flow cytometry technique. RESULTS: The results of this study showed that the gene expression of IL-37 in blood samples of metastatic and non-metastatic breast cancer patients was significantly lower than in healthy individuals (P < 0.05). The relative gene expression of the IL-37 in ER+/PR+/HER2+ patients with non-metastatic breast cancer had a significant increase compared to HER2+ patients (P < 0.05). Also, CD8 T lymphocytes count in the samples of patients including non-metastatic and metastatic breast cancer was significantly decreased compared to the healthy individuals (P < 0.05). CONCLUSIONS: Our findings provide evidence that IL-37 gene expression and CD8 T lymphocytes count, significantly decreased in non-metastatic and metastatic breast cancer. Considering the possible effects of IL-37 on TCD8 cells in tumor immune responses, more research will be done to benefit from the therapeutic effects of this cytokine in the future.
The Antibacterial Activity of Thermoactinomyces sp. (H24) Extract Against Escherichia coli and Staphylococcus aureus
Authors: Puspita Julia Nanda , Kurniatuhadi Rikhsan , Rahmawati Rahmawati 
Published: May 2021

Bacteria of the genus Thermoactinomyces have the ability to produce antibacterial bioactive compounds. This bioactive compound can be used for combating diarrheal agents such as Escherichia coli and Staphylococcus aureus. This study aims to determine the antibacterial activity of the metabolite extract from Thermoactinomyces sp. (H24) against Escherichia coli and Staphylococcus aureus. Methanol was used as a solvent for the extraction of bacterial bioactive compounds. Antibacterial activity was analyzed by the diffusion method with several extract concentrations (0.75 mL, 1.5 mL, 2.25 mL, and 3 mL), 10% DMSO as the negative control, and ciprofloxacin as the positive control. Our result shows that Termoactinomyces sp. (H24) extract has an inhibitory effect on the growth of E. coli and S. aureus with an effective concentration of 2.25 mL (inhibition strength: very strong).
Enhanced mPGES-1 Contributes to PD-Related Peritoneal Fibrosis via Activation of the NLRP3 Inflammasome
Authors: Luo Qimei , Hu Qinghua , Zheng Qingkun , Gong Lewei , Su Lijuan , Ren Baojun , Ju Yongle , Jia Zhanjun , Dou Xianrui 
Published: May 2021

Background: Microsomal prostaglandin E synthase-1 (mPGES-1)-derived prostaglandin E2 (PGE2) is a chief mediator of inflammation. However, the role and mechanism of mPGES-1 in peritoneal dialysis (PD)-associated peritoneal fibrosis have not been investigated.Material and Methods: In PD patients, mPGES-1 expression in peritoneum tissues and the levels of PGE2, IL-1β, and IL-18 in the dialysate were examined. In rat peritoneal mesothelial cells (RPMCs), the regulation and function of mPGES-1 and NLRP3 inflammasome were investigated. The expression of extracellular matrix proteins and the components of NLRP3 inflammasome were detected by Western blotting or real-time quantitative PCR.Results: In PD patients with ultrafiltration failure (UFF), mPGES-1 was enhanced in the peritoneum, which was associated with the degree of peritoneal fibrosis. Accordingly, the intraperitoneal PGE2 levels were also positively related to the PD duration, serum C-reactive protein levels, and serum creatinine levels in incident PD patients. In RPMCs, high-glucose treatment significantly induced mPGES-1 expression and PGE2 secretion without affecting the expressions of mPGES-2 and cPGES. Inhibition of mPGES-1 via short hairpin RNA significantly ameliorated the expression of extracellular matrix proteins of RPMCs induced by high glucose. Additionally, high glucose markedly activated NLRP3 inflammasome in RPMCs that was blunted by mPGES-1 inhibition. Furthermore, silencing NLRP3 with siRNA significantly abrogated the expression of extracellular matrix proteins in RPMCs treated with high glucose. Finally, we observed increased IL-1β and IL-18 levels in the dialysate of incident PD patients, showing a positive correlation with PGE2.Conclusion: These data demonstrate that mPGES-1-derived PGE2 plays a critical role in PD-associated peritoneal fibrosis through activation of the NLRP3 inflammasome. Targeting mPGES-1 may offer a novel strategy to treat peritoneal fibrosis during PD.
Multiple Omics Integration Reveals Key Circular RNAs in Hepatocellular Carcinoma
Authors: Huang Zi-Li , Huang Xiu-Yan , Huang Jin , Huang Xin-Yu , Xu Yong-Hua , Zhou Jian , Tang Zhao-You 
Published: May 2021

BackgroundHCC is one of the most common malignancies with an increasing incidence worldwide, especially in Asian countries. However, even though targeted cancer therapy drugs such as sorafenib and regorafenib are available, the overall outcome of HCC remains unsatisfactory. Thus, it is urgent to investigate the molecular mechanisms of HCC progression, so as to provide accurate diagnostic criteria and therapeutic targets.MethodsRNA-seq data was used to identify and quantify circular RNAs (circRNAs). DESeq2 was used to identify the differentially expressed circRNAs. miRNA binding sites within circRNAs were identified by miRanda. Gene set enrichment analysis (GSEA) was conducted to predict the biological function of circRNAs.ResultsThe differential expression analysis identified 107 upregulated and 95 downregulated circRNAs in HCC tissues. We observed that a differentially expressed circRNA (DE-circRNA), hsa_circ_0141900 was highly negatively correlated with its parental gene RAB1A (PCC &lt; -0.6), which was also closely associated with mTOR signaling pathway. Moreover, we also constructed competing endogenous RNA (ceRNA) network to identify key circRNAs involved in HCC. Notably, hsa_circ_0002130 and hsa_circ_0008774 were highly correlated with the genes involved in gluconeogenesis and HNF3A pathway via the target genes, GOT2 and AR, suggesting that the two circRNAs might regulate these pathways, respectively. Survival analysis revealed that GOT2 was associated with favorable prognosis. Furthermore, high expression of hsa_circ_0002130 was found to inhibit tumor cell growth and promotes GOT2 expression.ConclusionIn summary, the circRNAs highlighted by the integrative analysis greatly improved our understanding of the underlying mechanism of circRNAs in HCC.
Prognostic Significance of SUVmax Combined With Lactate Dehydrogenase in Advanced Lung Cancer Patients Treated With Immune Checkpoint Inhibitor Plus Chemotherapy: A Retrospective Study
Authors: Ke Linping , Wang Lu , Yu Jinming , Meng Xue 
Published: May 2021

PurposeThis research aims to investigate the predictive capacity of PET/CT quantitative parameters combined with haematological parameters in advanced lung cancer patients treated with immune checkpoint inhibitor (ICI) plus chemotherapy.MethodsA total of 120 patients who underwent 18F-fluorodeoxyglucose positron emission tomography/computed tomography (18F-FDG PET/CT) were enrolled before therapy. The following parameters were calculated: the maximum, mean, and peak standardized uptake value (SUVmax, SUVmean, and SUVpeak, respectively); total tumour volume (MTV) and total lesion glycolysis (TLG); and whole-body metabolic values (MTVwb, TLGwb, SUVmeanwb, and SUVmaxwb). Lactate dehydrogenase (LDH) levels, absolute neutrophil count, absolute platelet count, albumin levels and derived neutrophil to lymphocyte ratio (dNLR) were also computed. The associations between the variables and therapy outcome (evaluated by iRECIST) were analyzed.ResultsBased on iRECIST, 32 of 120 patients showed iPD, 43 iSD, 36 iPR and 9 iCR. Multivariate analysis found that SUVmax, MTVwb, LDH and absolute platelet count were associated with treatment response (P =0.015, P =0.005, P &lt;0.001 and P =0.015, respectively). Kaplan-Meier survival analyses showed that SUVmax ≥11.42 and LDH ≥245 U/L were associated with shorter OS (P = 0.001 and P = 0.004, respectively). Multivariate Cox regression revealed that SUVmax and LDH alone were not correlated with survival prognosis (p&gt;0.05), but the combination of SUVmax and LDH was independently associated with OS (P=0.015, P=0.001, respectively). The median survival time (MST) for the low (LDH&lt;245 and SUVmax&lt;11.42), intermediate(LDH&lt;245 or SUVmax&lt;11.42), and high(SUVmax≥11.42 and LDH≥245) groups was 24.10 months (95% CI: 19.43 to 28.77), 17.41 months (95% CI: 15.83 to 18.99), and 13.76 months (95% CI: 12.51 to 15.02), respectively.ConclusionThis study identified that SUVmax plus LDH correlated with the survival outcome in patients with advanced lung cancer receiving PD-1/PD-L1 blockade plus chemotherapy.
A Biosimilarity Study Between QX001S and Ustekinumab in Healthy Chinese Male Subjects
Authors: Gao Lei , Li Qingmei , Zhang Hong , Wu Min , Fang Min , Yang Lizhi , Li Xiaojiao , Liu Jingrui , Li Cuiyun , Chen Hong , Zhu Xiaoxue , Ding Yanhua , Zhou Mingwei 
Published: May 2021

Objective: To evaluate the tolerance, variability, and pharmacokinetics (PK) of QX001S, a biosimilar for ustekinumab, in healthy Chinese men.Methods: One hundred and seventy-eight healthy men were recruited in this randomized, double-blind, single-dose, two-arm, parallel study, and received 45 mg of QX001S or ustekinumab in a single subcutaneous injection. PK, immunogenicity, and tolerance were evaluated in all participants for a period of 113°days.Results: The similarity between the two drugs was determined by comparing the baseline characteristics for each drug. The PK parameters were similar in the two groups: QX001S (n = 89) and ustekinumab (n = 88). The 90% confidence intervals (CIs) for the geometric mean ratio (GMR) of QX001S to the reference (ustekinumab) for the maximum observable serum concentration (Cmax), area under the curve (AUC) from zero to the final quantifiable concentration (AUC0–t), and AUC from zero to infinity (AUC0–∞) were 100.90–118.68%, 98.71–115.26%, and 98.49–115.81%, respectively, which were within the predefined bioequivalence limit of 80.00–125.00%. High inter-subject variability (ranging from 32.0 to 33.5%) was observed. A total of 17 participants (19.1%) in the QX001S group and 36 (40.9%) in the ustekinumab group developed anti-drug antibodies (ADA) after administration. Nevertheless, the ADA did not affect the outcomes of the bioequivalence tests. Adverse reactions were recorded in 38 individuals injected with QX001S and 37 injected with ustekinumab. The most common adverse reactions were upper respiratory infection and elevated alanine aminotransferase.Conclusions: Our study ratified pharmacokinetic biosimilarity between QX001 S and ustekinumab, with high variability between subjects.
Effect of Blood Transfusion on Short- and Long-Term Outcomes in Oral Squamous Cell Carcinoma Patients Undergoing Free Flap Reconstruction
Authors: Feng Aimin , Zhang Jiaqiang , Lu Xihua , Fang Qigen 
Published: May 2021

Purpose: To analyze the short- and long-term effect of perioperative blood transfusion (PBT) in patients undergoing surgical treatment for oral squamous cell carcinoma (SCC).Methods: Patients undergoing free flap reconstruction were retrospectively enrolled and divided into two groups based on the implementation of PBT. Flap revision, surgical site infection (SSI), flap failure, overall survival (OS), and disease-specific survival (DSS) were compared between the two groups.Results: In 170 patients with PBT, 10 (5.9%) flaps required exploration revision, SSI occurred in 18 (10.6%) patients, and flap necrosis was noted in 6 (3.5%) patients. These rates were comparable to those in patients without PBT. The two groups had similar DSS rates, but the 5-year OS rates were 49 and 59% in patients with PBT and without PBT, respectively. This difference was significant. Patients with 4 units of PBT had OS rates comparable to those of patients with &gt;4 units of PBT. A Cox model confirmed the fact that the decrease in OS was independent of PBT.Conclusion: In patients with free flap reconstruction for oral SCC, PBT did not increase the short-term complication rate or cancer-linked mortality. However, it was related to an elevated overall risk of death.
Understanding Vaccine Hesitancy in the Context of COVID-19: The Role of Trust and Confidence in a Seventeen-Country Survey
Authors: Rozek Laura S. , Jones Pauline , Menon Anil , Hicken Allen , Apsley Samantha , King Elizabeth J. 
Published: May 2021

Objectives: An effective vaccine to SARS-CoV-2 cannot be successfully deployed if a significant number of people worldwide are unwilling to accept it. We investigated the relationship between trust in scientists and medical professionals and perceptions of vaccine safety and effectiveness. We also build on past studies by exploring the relationship between confidence in global health organizations and vaccine hesitancy.Methods: We conducted an online survey in seventeen countries/territories across five world regions between May -June 2020. We assessed the relationship between COVID19 vaccine hesitancy, confidence in public health organizations, and trust in key experts and leaders.Results: Our findings strongly suggest that confidence in the World Health Organization combined with trust in domestic scientists and healthcare professionals is a strong driver of vaccine acceptance across multiple countries/territories.Conclusion: We find that hesitancy is widespread, and uptake would be insufficient to achieve herd immunity. There is widespread confidence in how public health organizations have responded to the current pandemic and this is related to vaccine acceptance. Our results also highlight the important role of trust in health care providers and scientists in reducing COVID19 vaccine hesitancy.
Integrating primary care and social services for older adults with multimorbidity: a qualitative study
Authors: Dambha-Miller Hajira , Simpson Glenn , Hobson Lucy , Olaniyan Doyinsola , Hodgson Sam , Roderick Paul , Fraser Simon DS , Little Paul , Everitt Hazel , Santer Miriam 
Published: May 2021

BackgroundGrowing demand from an increasingly ageing population with multimorbidity has resulted in complex health and social care needs requiring more integrated services. Integrating primary care with social services could utilise resources more efficiently, and improve experiences for patients, their families, and carers. There is limited evidence on progress including key barriers to and drivers of integration to inform large-scale national change.AimTo elicit stakeholder views on drivers and barriers of integrated primary care and social services, and highlight opportunities for successful implementation.Design and settingA qualitative interview study.MethodSemi-structured interviews with maximum variation sampling to capture stakeholder views across services and professions.ResultsThirty-seven interviews were conducted across England with people including GPs, nurses, social care staff, commissioners, local government officials, voluntary and private sector workers, patients, and carers. Drivers of integration included groups of like-minded individuals supported by good leadership, expanded interface roles to bridge gaps between systems, and co-location of services. Barriers included structural and interdisciplinary tension between professions, organisational self-interest, and challenges in record sharing.ConclusionDrivers and barriers to integration identified in other contexts are also present in primary care and social services. Benefits of integration are unlikely to be realised if these are not addressed in the design and execution of new initiatives. Efforts should go beyond local- and professional-level change to include wider systems- and policy-level initiatives. This will support a more systems-wide approach to integrated care reform, which is necessary to meet the complex and growing needs of an ageing multimorbid population.
GOOD CORPORATE GOVERNANCE SEBAGAI PEMODERASI: HUBUNGAN ASIMETRI INFORMASI TERHADAP MANAJEMEN LABA PADA PERUSAHAAN LQ 45
Authors: Tangngisalu Jannati , Jumady Edy 
Published: May 2021

Abstract. This study aims to determine whether good corporate governance is able to strengthen/weaken the influence of information asymmetry on earnings management. Data collection uses secondary data obtained from financial statements using saturated sampling techniques. The population is all companies listed on the LQ 45 Indonesia Stock Exchange (IDX) of 45 companies, and all companies are used as samples. The results of this study have tested classic assumptions and data analysis methods using multiple linear regression techniques and moderating regression analysis (MRA).The results show that information asymmetry has no significant positive effect on earnings management, which means that earnings management is no longer determined by information asymmetry but is determined by other factors. While the variable of good corporate governance showed significant negative results. This means that good corporate governance can weaken or reduce the effect of information asymmetry on earnings management. So with the decline in information asymmetry in the company, earnings management actions also decline. Abstrak. Penelitian ini bertujuan untuk mengetahui apakah good corporate governance mampu memperkuat / memperlemah pengaruh asimetri informasi terhadap manajemen laba. Pengumpulan data menggunakan data sekunder yang diperoleh dari laporan keuangan dengan menggunakan teknik sampling jenu. Populasinya adalah semua perusahaan yang terdaftar di Bursa Efek LQ 45 Indonesia (IDX) yang berjumlah 45 perusahaan, dan semua perusahaan digunakan sebagai sampel. Hasil penelitian ini telah diuji asumsi klasik dan metode analisis data menggunakan teknik regresi linier berganda dan moderating regression analysis (MRA).Hasil penelitian menunjukkan bahwa asimetri informasi berpengaruh positif tidak signifikan terhadap manajemen laba, yang berarti bahwa manajemen laba tidak lagi ditentukan oleh asimetri informasi tetapi ditentukan oleh faktor lain. Sedangkan variabel good corporate governance menunjukkan hasil negatif signifikan. Ini berarti bahwa good corporate governance mampu melemahkan atau menurunkan pengaruh asimetri informasi pada manajemen laba. Jadi dengan menurunnya asimetri informasi dalam perusahaan maka tindakan manajemen laba ikut menurun.Keywords: Information Asymmetry, Profit Management, Good Corporate Governance.
BLACK SWAN GLOBAL MARKET: ANALYSIS OF THE EFFECT OF THE COVID-19 DEATH RATE ON THE VOLATILITY OF EUROPEAN FOOTBALL CLUB STOCK PRICES (CASE STUDY OF JUVENTUS F.C., MANCHESTER UNITED, AJAX AMSTERDAM AND BORUSSIA DORTMUND)
Authors: Limba Franco Benony , Rijoly Jacobus Cliff Diky , Tarangi Margreath I 
Published: May 2021

Abstract: The Covid-19 pandemic that hit the world also directly affected financial markets and global stock markets; this condition in economic terminology is known as the Black Swann Global Market Effect. Black Swan Global Market Effect is also experienced by sports industries in the financial industry, the football industry. The purpose of this paper is to see whether there is an influence between the Covid-19 pandemic conditions on the share value of several major European football clubs, namely Ajax Amsterdam, Borussia Dortmund, Juventus F.C., and Manchester United, as a result of the Black Swan Global Market Effect. The data used in this paper is time-series data from March 2020 to August 2020. Meanwhile, to answer the black swan effect phenomenon, the Threshold Generalized Autoregressive Conditional Heteroskedasticity (TGARCH) method is used. The results showed that stocks that were the object of research (Ajax, Borussia Dortmund, Juventus, and Machester United) showed a large response to bad News (an increase in deaths due to covid-19). Abstrak:Pandemic covid-19 yang mengantam dunia juga secara langsung mempengaruhi pasar keuangan serta pasar saham global, kondisi ini dalam terminology ekonomi dikenal sebagai Black Swann Global Markert Effect. Black Swan Global Market Effect hal ini juga dialami industry-industri olahraga yang berada dalam industry keuangan tersebut salah satunya industry sepakbola.Tujuan penulisan ini adalah untuk melihat apakah terdapat pengaruh antara kondisi pandemic covid-19 terhadap nilai saham beberapa klub sepakbola besar eropa yaitu Ajax Amsterdam, Borussia Dortmund, Juventus FC, dan Manchester United sebagai akibat dari Black Swan Global Market Effect.Data yang digunakan dalam penulisan ini adalah data time series dari bulan maret 2020 hingga agustus 2020. Sementara untuk menjawab fenomoena black swan effect ini digunakan metode Threshold Generalized Autoregressive Conditional Heteroskedacity (TGARCH). Hasil Penelitian menunjukkan bahwa, saham-saham yang menjadi objek penelitian (Ajax, Borussia Dortmund, Juventus, dan Machester United) menunjukan respons yang besar terhadap bad news (peningkatan jumlah kematian akibat covid-19). Black Swan Global Market, Pandemi Covid-19, TGARCH Models
ANALYSIS OF FACTORS THAT ARE CONSIDERED BY INVESTORS IN STOCKS INVESTMENT DECISION MAKING IN BATAM CITY
Authors: Cuandra Fendy , Tan Helen 
Published: May 2021

Abstract:The purpose of this research is to analyze the factors that investors should consider in stock investment decision making. This research was conducted to assess the interest in investing and consideration when investing which is influenced by representative bias, availability bias, and locus of control. This research was tested using Smart PLS application. Data collection from this study was conducted by distributing questionnaires as many as 353 people in Batam city. Representative bias significantly positively affects investment decision making, availability bias significantly and positively in investment decisions, availability bias moderated locus of control has no effect on investment decision making, and representative bias moderated locus of control has no effect on investment decision making. This research facilitates investors in making investment decisions in Batam city, judging from several factors that influence the decision making helps investors in determining investment and considering every factor that influences Decision making. Abstrak: Tujuan dari penelitian ini adalah untuk menganalisa faktor yang harus dipertimbangkan investor dalam pengambilan keputusan investasi Saham. Penelitian ini dilakukan untuk menilai ketertarikan dalam melakukan investasi dan pertimbangan saat berinvestasi yang dipengaruhi oleh representative bias, availability bias, dan locus of control. Penelitian ini di uji dengan menggunakan aplikasi Smart PLS. Pengumpulan data dari penelitian ini dilakukan dengan cara menyebarkan kuesioner sebanyak 353 orang yang berada kota Batam. Representative bias secara signifikan positif mempengaruhi investment desicion making, availability bias secara signifikan dan positif dalam keputusan investasi, availability bias yang dimoderasi locus of control tidak berpengaruh terhadap investment decision making, dan representative bias yang dimoderasi locus of control tidak berpengaruh terhadap investment decision making. Penelitian ini memberi kemudahan investor dalam mengambil keputusan investasi di kota Batam, dilihat dari beberapa faktor yang mempengaruhi pengambilan keputusan ini membantu para investor dalam menentukan investasi dan mempertimbangkan setiap faktor yang mempengaruhi Decision making.