bluuebunny/crossref_metadata_2025_split huggingface dataset:
Clinicopathological impacts of DNA methylation alterations on pancreatic ductal adenocarcinoma: prediction of early recurrence based on genome-wide DNA methylation profiling
Authors: Endo Yutaka , Fujimoto Mao , Ito Nanako , Takahashi Yoriko , Kitago Minoru , Gotoh Masahiro , Hiraoka Nobuyoshi , Yoshida Teruhiko , Kitagawa Yuko , Kanai Yae , Arai Eri 
Published: February 2021

Abstract
Purpose
The present study was conducted to clarify the clinicopathological impacts of DNA methylation alterations on pancreatic ductal adenocarcinoma (PDAC).

Methods
Genome-wide DNA methylation screening was performed using the Infinium HumanMethylation450 BeadChip, and DNA methylation quantification was verified using pyrosequencing. We analyzed fresh-frozen tissues from an initial cohort (17 samples of normal control pancreatic tissue [C] from 17 patients without PDAC, and 34 samples of non-cancerous pancreatic tissue [N] and 82 samples of cancerous tissue [T] both obtained from 82 PDAC patients) and formalin-fixed paraffin-embedded T samples from 34 patients in a validation cohort.

Results
The DNA methylation profiles of N samples tended to differ from those of C samples, and 91,907 probes showed significant differences in DNA methylation levels between C and T samples. Epigenetic clustering of T samples was significantly correlated with a larger tumor diameter and early recurrence (ER), defined as relapse within 6 months after surgery. Three marker CpG sites, applicable to formalin-fixed paraffin-embedded surgically resected materials regardless of their tumor cell content, were identified for prediction of ER. The sensitivity and specificity for detection of patients belonging to the ER group using a panel combining these three marker CpG sites, including a CpG site in the CDK14 gene, were 81.8% and 71.7% and 88.9% and 70.4% in the initial and validation cohorts, respectively.

Conclusion
These findings indicate that DNA methylation alterations may have a clinicopathological impact on PDAC. Application of our criteria will ultimately allow prediction of ER after surgery to improve the outcome of PDAC patients.
Dispersing Obnoxious Facilities on a Graph
Authors: Grigoriev Alexander , Hartmann Tim A. , Lendl Stefan , Woeginger Gerhard J. 
Published: February 2021

AbstractWe study a continuous facility location problem on a graph where all edges have unit length and where the facilities may also be positioned in the interior of the edges. The goal is to position as many facilities as possible subject to the condition that any two facilities have at least distance $$\delta$$
δ
from each other. We investigate the complexity of this problem in terms of the rational parameter $$\delta$$
δ
. The problem is polynomially solvable, if the numerator of $$\delta$$
δ
is 1 or 2, while all other cases turn out to be NP-hard.
Robotic-assisted cholecystectomy is superior to laparoscopic cholecystectomy in the initial training for surgical novices in an ex vivo porcine model: a randomized crossover study
Authors: Willuth E. , Hardon S. F. , Lang F. , Haney C. M. , Felinska E. A. , Kowalewski K. F. , Müller-Stich B. P. , Horeman T. , Nickel F. 
Published: February 2021

Abstract
Background
Robotic-assisted surgery (RAS) potentially reduces workload and shortens the surgical learning curve compared to conventional laparoscopy (CL). The present study aimed to compare robotic-assisted cholecystectomy (RAC) to laparoscopic cholecystectomy (LC) in the initial learning phase for novices.

Methods
In a randomized crossover study, medical students (n = 40) in their clinical years performed both LC and RAC on a cadaveric porcine model. After standardized instructions and basic skill training, group 1 started with RAC and then performed LC, while group 2 started with LC and then performed RAC. The primary endpoint was surgical performance measured with Objective Structured Assessment of Technical Skills (OSATS) score, secondary endpoints included operating time, complications (liver damage, gallbladder perforations, vessel damage), force applied to tissue, and subjective workload assessment.

Results
Surgical performance was better for RAC than for LC for total OSATS (RAC = 77.4 ± 7.9 vs. LC = 73.8 ± 9.4; p = 0.025, global OSATS (RAC = 27.2 ± 1.0 vs. LC = 26.5 ± 1.6; p = 0.012, and task specific OSATS score (RAC = 50.5 ± 7.5 vs. LC = 47.1 ± 8.5; p = 0.037). There were less complications with RAC than with LC (10 (25.6%) vs. 26 (65.0%), p = 0.006) but no difference in operating times (RAC = 77.0 ± 15.3 vs. LC = 75.5 ± 15.3 min; p = 0.517). Force applied to tissue was similar. Students found RAC less physical demanding and less frustrating than LC.

Conclusions
Novices performed their first cholecystectomies with better performance and less complications with RAS than with CL, while operating time showed no differences. Students perceived less subjective workload for RAS than for CL. Unlike our expectations, the lack of haptic feedback on the robotic system did not lead to higher force application during RAC than LC and did not increase tissue damage. These results show potential advantages for RAS over CL for surgical novices while performing their first RAC and LC using an ex vivo cadaveric porcine model.

Registration number
researchregistry6029

Graphic abstract
Optimal ICG dosage of preoperative colonoscopic tattooing for fluorescence-guided laparoscopic colorectal surgery
Authors: Ahn Hong-min , Son Gyung Mo , Lee In Young , Shin Dong-Hoon , Kim Tae Kyun , Park Su Bum , Kim Hyung Wook 
Published: February 2021

Abstract
Background
Indocyanine green (ICG) is a multifunctional dye used in tumor localization, tissue perfusion, and lymph node (LN) mapping during fluorescence-guided laparoscopic colorectal surgery.

Purpose
This study aimed to establish the optimal protocol for preoperative endoscopic submucosal ICG injection to perform fluorescence lymph node mapping (FLNM), along with undisturbed fluorescent tumor localization and ICG angiography during a single surgery.

Methods
Colorectal cancer patients (n = 192) were enrolled from May 2017 to December 2019. Colonoscopic submucosal ICG injection was performed 12 to 18 h before surgery. ICG injection protocols were modified based on the total injected ICG (mg) and tattooing site number. The concentrations of ICG were gradually decreased from the standard dose (2.5 mg/ml) to the minimum dose (0.2 mg/ml). Successful FLNM (FLNM-s) was defined as distinct fluorescent LNs observed under NIR camera. The patient’s age, sex, body mass index (BMI), stage, cancer location, obstruction, and laboratory findings were compared between the FLNM-s and failed FLNM (FLNM-f) groups to identify clinical and pathological factors that affect FLNM.

Results
In the ICG dose section of 0.5 to 1 mg, the success rate was highest within all functions including FLNM, fluorescent tumor localization, and ICG angiography. FLNM-s was related to ICG dose (0.5–1 mg), multiple submucosal injections, location of cancer, camera light source, and lower BMI. In the multivariate analysis, camera light source, non-obesity, and multiple injections were independent factors for FLNM-s). The mean total number of harvested LNs was significantly higher in the FLNM-s group than that in the FLNM-f group (p < 0.001). The number of metastatic lymph nodes was comparable between the two groups (p = 0.859).

Conclusions
Preoperative, endoscopic submucosal ICG injection with dose range 0.5 to 1 mg would be optimal protocol for multifunctional ICG applications during fluorescence-guided laparoscopic colorectal surgery.
Schizophrenia polygenic risk is associated with child mental health problems through early childhood adversity: evidence for a gene–environment correlation
Authors: Bolhuis Koen , Steenkamp Lisa R. , Blanken Laura M. E. , Neumann Alexander , Jansen Philip R. , Hillegers Manon H. J. , Cecil Charlotte A. M. , Tiemeier Henning , Kushner Steven A. 
Published: February 2021

AbstractPrevious studies have shown that schizophrenia polygenic risk predicts a multitude of mental health problems in the general population. Yet it is unclear by which mechanisms these associations arise. Here, we explored a possible gene–environment correlation in the association of schizophrenia polygenic risk with mental health problems via childhood adversity. This study was embedded in the population-based Generation R Study, including N = 1901 participants with genotyping for schizophrenia polygenic risk, maternal reporting of childhood adversity, and Child Behaviour Checklist measurement of mental health problems. Independent replication was attempted in the Avon Longitudinal Study of Parents and Children (ALSPAC; N = 3641). Associations were analysed with Poisson regression and statistical mediation analysis. Higher burden of schizophrenia polygenic risk was associated with greater exposure to childhood adversity (P-value threshold < 0.5: Generation R Study, OR = 1.08, 95%CI 1.02–1.15, P = 0.01; ALSPAC, OR = 1.02, 95%CI 1.01–1.03, P < 0.01). Childhood adversities partly explained the relationship of schizophrenia polygenic risk with emotional, attention, and thought problems (proportion explained, range 5–23%). Direct effects of schizophrenia polygenic risk and adversity on mental health outcomes were also observed. In summary, genetic liability to schizophrenia increased the risk for mental health problems in the general paediatric population through childhood adversity. Although this finding could result from a mediated causal relationship between genotype and mental health, we argue that these observations most likely reflect a gene–environment correlation, i.e. adversities are a marker for the genetic risk that parents transmit to children. These and similar recent findings raise important conceptual questions about preventative interventions aimed at reducing childhood adversities.
Holistic principle for risk aggregation and capital allocation
Authors: Chong Wing Fung , Feng Runhuan , Jin Longhao 
Published: February 2021

AbstractRisk aggregation and capital allocation are of paramount importance in business, as they play critical roles in pricing, risk management, project financing, performance management, regulatory supervision, etc. The state-of-the-art practice often includes two steps: (i) determine standalone capital requirements for individual business lines and aggregate them at a corporate level; and (ii) allocate the total capital back to individual lines of business or at more granular levels. There are three pitfalls with such a practice, namely, lack of consistency, negligence of cost of capital, and disentanglement of allocated capitals from standalone capitals. In this paper, we introduce a holistic approach that aims to strike a balance of optimality by taking into account competing interests of various stakeholders and conflicting priorities in a corporate hierarchy. While unconventional in its objective, the new approach results in an allocation of diversification benefit, which conforms to the diversification strategy of many risk management frameworks including regulatory capital and economic capital. The holistic capital setting and allocation principle provides a remedy to aforementioned problems with the existing two-step industry practice.
Morphologies and prognostic significance of left ventricular volume/time curves with cardiac magnetic resonance in patients with non-ischaemic heart failure and left bundle branch block
Authors: Aimo Alberto , Valleggi Alessandro , Barison Andrea , Salerni Sara , Emdin Michele , Aquaro Giovanni Donato 
Published: February 2021

AbstractPatients with non-ischaemic systolic heart failure (HF) and left bundle branch block (LBBB) can display a wide or narrow pattern (WP/NP) of the systolic phase of the left ventricular (LV) volume/time (V/t) curve in cardiac magnetic resonance (CMR). The clinical and prognostic significance of these patterns is unknown. Consecutive patients with non-ischaemic HF, LV ejection fraction < 50% and LBBB underwent 1.5 T CMR. Maximal dyssynchrony time (time between the earliest and latest end-systolic peaks), systolic dyssynchrony index (standard deviation of times to peak volume change), and contractility index (maximum rate of change of pressure-normalized stress) were calculated. The endpoint was a composite of cardiovascular death, HF hospitalization, and appropriate defibrillator shock. NP was found in 29 and WP in 72 patients. WP patients had higher volumes and NT-proBNP, and lower LVEF. WP patients had a longer maximal dyssynchrony time (absolute duration: 192 ± 80 vs. 143 ± 65 ms, p < 0.001; % of RR interval: 25 ± 11% vs. 8 ± 4%, p < 0.001), a higher systolic dyssynchrony index (13 ± 4 vs. 7 ± 3%, p < 0.001), and a lower contractility index (2.6 ± 1.2 vs 3.2 ± 1.7, p < 0.05). WP patients had a shorter survival free from the composite endpoint regardless of age, NT-proBNP or LVEF. Nonetheless, WP patients responded more often to cardiac resynchronization therapy (CRT) than those with NP (24/28 [86%] vs. 1/11 [9%] responders, respectively; p < 0.001). In patients with non-ischaemic systolic HF and LBBB, the WP of V/t curves identifies a subgroup of patients with greater LV dyssynchrony and worse outcome, but better response to CRT.
Value sensitive design as a formative framework
Authors: Hendry David G. , Friedman Batya , Ballard Stephanie 
Published: February 2021

AbstractIn this article, we first offer a model of design knowledge types (know-about, know-that, know-how) and their interrelationships in value sensitive design. Then we demonstrate that value sensitive design is a formative framework, which provides a shaping influence on practice, enables creative appropriation, and supports theory and method development.
Development of the PARMS marker of the TAC1 gene and its utilization in rice plant architecture breeding
Authors: Gao Ju , Liang Haifu , Huang Juan , Qing Dongjin , Wu Hao , Zhou Weiyong , Chen Weiwei , Pan Yinghua , Dai Gaoxing , Gao Lijun , Deng Guofu 
Published: February 2021

AbstractThe ideal plant architecture is a new strategy for super high yield breeding of rice. Tiller angle is an important plant architecture character of rice. A reasonable tiller angle is a key factor for the ideal plant architecture and achieving high-yield breeding. Molecular design breeding is the most potential new direction of crop breeding in the future. The development of accurate and efficient functional molecular markers of target trait genes is crucial for molecular design breeding. The TAC1 (Tiller Angle Controlling) gene is the primary gene that regulates tiller angle in rice. This gene can be used to improve the compact plant architecture of indica and japonica rice varieties. The SNP variation from A to G at the fourth intron 3′ splicing point in TAC1 changes plant architecture. Based on the SNP variation, PM-TAC1 was successfully developed as a fluorescent functional molecular marker, via the penta-primer amplification refractory mutation system. Ninety-three rice materials were genotyped using this marker, and the marker was effectively used in rice plant architecture breeding. The successful development of this marker will contribute to the molecular breeding of rice plant architecture.
Proportion of children with cancer that have an indication for genetic counseling and testing based on the cancer type irrespective of other features
Authors: Nguyen Thi Minh Kha , Behnert Astrid , Pietsch Torsten , Vokuhl Christian , Kratz Christian Peter 
Published: February 2021

Abstract In children with cancer, specific clinical features such as physical anomalies, occurrence of cancer in young relatives, specific cancer histologies, and unique mutation/methylation signatures may indicate the presence of an underlying cancer predisposition syndrome (CPS). The proportion of children with a cancer type suggesting a CPS among all children with cancer is unknown. To determine the proportion of children with cancer types suggesting an underlying CPS among children with cancer. We evaluated the number of children with cancer types strongly associated with CPS diagnosed in Germany between 2007 and 2016. Data were obtained from various sources including two national pediatric pathology reference laboratories for brain and solid tumors, respectively, various childhood cancer trial offices as well as the German Childhood Cancer Registry. Among 21,127 children diagnosed with cancer between 2007 and 2016, 2554 (12.1%) had a cancer type strongly associated with a CPS. The most common diagnoses were myelodysplastic syndrome and juvenile myelomonocytic leukemia, retinoblastoma, malignant peripheral nerve sheath tumor, infantile myofibromatosis, medulloblastomaSHH, rhabdoid tumor as well as atypical teratoid/rhabdoid tumor. Based on cancer type only, 12.1% of all children with cancer have an indication for a genetic evaluation. Pediatric oncology patients require access to genetic counselling and testing.
Hardware-in-the-loop simulation of wave energy converters based on dielectric elastomer generators
Authors: Moretti Giacomo , Scialò Andrea , Malara Giovanni , Muscolo Giovanni Gerardo , Arena Felice , Vertechy Rocco , Fontana Marco 
Published: February 2021

AbstractDielectric elastomer generators (DEGs) are soft electrostatic generators based on low-cost electroactive polymer materials. These devices have attracted the attention of the marine energy community as a promising solution to implement economically viable wave energy converters (WECs). This paper introduces a hardware-in-the-loop (HIL) simulation framework for a class of WECs that combines the concept of the oscillating water columns (OWCs) with the DEGs. The proposed HIL system replicates in a laboratory environment the realistic operating conditions of an OWC/DEG plant, while drastically reducing the experimental burden compared to wave tank or sea tests. The HIL simulator is driven by a closed-loop real-time hydrodynamic model that is based on a novel coupling criterion which allows rendering a realistic dynamic response for a diversity of scenarios, including large scale DEG plants, whose dimensions and topologies are largely different from those available in the HIL setup. A case study is also introduced, which simulates the application of DEGs on an OWC plant installed in a mild real sea laboratory test-site. Comparisons with available real sea-test data demonstrated the ability of the HIL setup to effectively replicate a realistic operating scenario. The insights gathered on the promising performance of the analysed OWC/DEG systems pave the way to pursue further sea trials in the future.
Always look on the bright side of life? Exploring the between-variance and within-variance of emotion regulation goals
Authors: Wilms R. , Lanwehr R. , Kastenmüller A. 
Published: February 2021

AbstractCurrently, emotion regulation goals are being perceived as highly situational. This assumption might be wrong, though, as the preeminent measure [the intraclass coefficient (1), ICC(1)] overestimates the proportion of within-variance under the condition of measurement error. We therefore empirically test whether emotion regulation goals represent more of a between-person or a within-person phenomenon, using the reliability-adjusted ICC(1). A total of 305 students participated in a daily diary study and answered a questionnaire about their emotion regulation goals in the most negative event of the day over the course of 9 days. Multilevel analyses suggest that emotion regulation goals vary more between persons than heretofore assumed, especially for hedonic goals, but also for social goals. Besides, we show substantial differences in the within-variance across individuals. We conclude by discussing theoretical implications for general and clinical psychology.
Geodesy, Geophysics and Fundamental Physics Investigations of the BepiColombo Mission
Authors: Genova Antonio , Hussmann Hauke , Van Hoolst Tim , Heyner Daniel , Iess Luciano , Santoli Francesco , Thomas Nicolas , Cappuccio Paolo , di Stefano Ivan , Kolhey Patrick , Langlais Benoit , Mieth Johannes Z. D. , Oliveira Joana S. , Stark Alexander , Steinbrügge Gregor , Tosi Nicola , Wicht Johannes , Benkhoff Johannes 
Published: February 2021

AbstractIn preparation for the ESA/JAXA BepiColombo mission to Mercury, thematic working groups had been established for coordinating the activities within the BepiColombo Science Working Team in specific fields. Here we describe the scientific goals of the Geodesy and Geophysics Working Group (GGWG) that aims at addressing fundamental questions regarding Mercury’s internal structure and evolution. This multidisciplinary investigation will also test the gravity laws by using the planet Mercury as a proof mass. The instruments on the Mercury Planetary Orbiter (MPO), which are devoted to accomplishing the GGWG science objectives, include the BepiColombo Laser Altimeter (BELA), the Mercury orbiter radio science experiment (MORE), and the MPO magnetometer (MPO-MAG). The onboard Italian spring accelerometer (ISA) will greatly aid the orbit reconstruction needed by the gravity investigation and laser altimetry. We report the current knowledge on the geophysics, geodesy, and evolution of Mercury after the successful NASA mission MESSENGER and set the prospects for the BepiColombo science investigations based on the latest findings on Mercury’s interior. The MPO spacecraft of the BepiColombo mission will provide extremely accurate measurements of Mercury’s topography, gravity, and magnetic field, extending and improving MESSENGER data coverage, in particular in the southern hemisphere. Furthermore, the dual-spacecraft configuration of the BepiColombo mission with the Mio spacecraft at higher altitudes than the MPO spacecraft will be fundamental for decoupling the internal and external contributions of Mercury’s magnetic field. Thanks to the synergy between the geophysical instrument suite and to the complementary instruments dedicated to the investigations on Mercury’s surface, composition, and environment, the BepiColombo mission is poised to advance our understanding of the interior and evolution of the innermost planet of the solar system.
Intra-Camera Supervised Person Re-Identification
Authors: Zhu Xiangping , Zhu Xiatian , Li Minxian , Morerio Pietro , Murino Vittorio , Gong Shaogang 
Published: February 2021

AbstractExisting person re-identification (re-id) methods mostly exploit a large set of cross-camera identity labelled training data. This requires a tedious data collection and annotation process, leading to poor scalability in practical re-id applications. On the other hand unsupervised re-id methods do not need identity label information, but they usually suffer from much inferior and insufficient model performance. To overcome these fundamental limitations, we propose a novel person re-identification paradigm based on an idea ofindependentper-camera identity annotation. This eliminates the most time-consuming and tedious inter-camera identity labelling process, significantly reducing the amount of human annotation efforts. Consequently, it gives rise to a more scalable and more feasible setting, which we callIntra-Camera Supervised (ICS)person re-id, for which we formulate a Multi-tAsk mulTi-labEl (MATE) deep learning method. Specifically, MATE is designed for self-discovering the cross-camera identity correspondence in a per-camera multi-task inference framework. Extensive experiments demonstrate the cost-effectiveness superiority of our method over the alternative approaches on three large person re-id datasets. For example, MATE yields 88.7% rank-1 score on Market-1501 in the proposed ICS person re-id setting, significantly outperforming unsupervised learning models and closely approaching conventional fully supervised learning competitors.