bluuebunny/crossref_metadata_2025_split huggingface dataset:
Longitudinal dynamics of clonal hematopoiesis identifies gene-specific fitness effects
Authors: Robertson Neil A. , Latorre-Crespo Eric , Terradas-Terradas Maria , Purcell Alison C. , Livesey Benjamin J , Marsh Joseph A. , Murphy Lee , Fawkes Angie , MacGillivray Louise , Copland Mhairi , Marioni Riccardo E. , Harris Sarah E. , Cox Simon R. , Deary Ian J. , Schumacher Linus J. , Kirschner Kristina , Chandra Tamir 
Published: May 2021

AbstractThe prevalence of clonal haematopoiesis of indeterminate potential (CHIP) in healthy individuals increases rapidly from age 60 onwards and has been associated with increased risk for malignancy, heart disease and ischemic stroke. CHIP is driven by somatic mutations in stem cells that are also drivers of myeloid malignancies. Since mutations in stem cells often drive leukaemia, we hypothesised that stem cell fitness substantially contributes to transformation from CHIP to leukaemia. Stem cell fitness is defined as the proliferative advantage over cells carrying no or only neutral mutations. It is currently unknown whether mutations in different CHIP genes lead to distinct fitness advantages that could form the basis for patient stratification. We set out to quantify the fitness effects of CHIP drivers over a 12 year timespan in older age, using longitudinal error-corrected sequencing data. We developed a new method based on drift-induced fluctuation (DIF) filtering to extract fitness effects from longitudinal data, and thus quantify the growth potential of variants within each individual. Our approach discriminates naturally drifting populations of cells and faster growing clones, while taking into account individual mutational context. We show that gene-specific fitness differences can outweigh inter-individual variation and therefore could form the basis for personalised clinical management.
AURKB/Ipl1 restarts replication forks to recover from replication stress
Authors: Moran Erin , Pfister Katherine , Sathyan Kizhakke Mattada , Vignero Brianne , Burke Daniel J. , Stukenberg P. Todd 
Published: May 2021

AbstractAurora kinase B (AURKB in human, Ipl1 inS. cerevisiae) is a master regulator of mitosis and its dysregulation has been implicated in chromosome instability. AURKB accumulates in the nucleus in S-phase and is regulated by CHK1 but has not been implicated in in the DNA Damage Response (DDR). Here we show that AURKB has a conserved role to recover from replication stress and restart replication forks. Active AURKB is localized to replication forks after a prolonged arrest. CHK1 phosphorylation of AURKB induces activating phosphorylation of PLK1 and both Aurora and Plk1 are required to deactivate the DDR. Clinical trials with AURKB inhibitors are designed to target established roles for AURKB in mitosis. Our data suggest combinations of AURKB inhibitors and DNA damaging agents could be of therapeutic importance.
Tissue homeostasis and adaptation to immune challenge resolved by fibroblast network mechanics
Authors: Horsnell Harry L. , Tetley Robert J. , De Belly Henry , Makris Spyridon , Millward Lindsey J. , Benjamin Agnesska C. , de Winde Charlotte M , Paluch Ewa K. , Mao Yanlan , Acton Sophie E. 
Published: May 2021

AbstractEmergent physical properties of tissues are not readily understood by reductionist studies of their constituent cells. Here, we show molecular signals controlling cellular physical properties, collectively determining tissue mechanics of lymph nodes, an immunologically-relevant, adult mammalian tissue. Lymph nodes paradoxically maintain robust tissue architecture in homeostasis yet are continually poised for extensive tissue expansion upon immune challenge. We find that following immune challenge, cytoskeletal mechanics of a cellular meshwork of fibroblasts determine tissue tension independently of extracellular matrix scaffolds. We determine that CLEC-2/podoplanin signalling regulates the cell surface mechanics of fibroblasts, permitting cell elongation and interdigitation through expedited access to plasma membrane reservoirs. Increased tissue tension through the stromal meshwork gates the initiation of fibroblast proliferation, restoring homeostatic cellular ratios and tissue structure through expansion.
Molecular Systems Predict Equilibrium Distributions of Phenotype Diversity Available for Selection
Authors: Valderrama-Gómez Miguel A. , Savageau Michael A. 
Published: May 2021

AbstractTwo long standing challenges in theoretical population genetics and evolution are predicting the distribution of phenotype diversity generated by mutation and available for selection and determining the interaction of mutation, selection, and drift to characterize evolutionary equilibria and dynamics. More fundamental for enabling such predictions is the current inability to causally link population genetic parameters, selection and mutation, to the underlying molecular parameters, kinetic and thermodynamic. Such predictions would also have implications for understanding cryptic genetic variation and the role of phenotypic robustness.Here we provide a new theoretical framework for addressing these challenges. It is built on Systems Design Space methods that relate system phenotypes to genetically-determined parameters and environmentally-determined variables. These methods, based on the foundation of biochemical kinetics and the deconstruction of complex systems into rigorously defined biochemical phenotypes, provide several innovations that automate (1) enumeration of the phenotypic repertoire without knowledge of kinetic parameter values, (2) representation of phenotypic regions and their relationships in a System Design Space, and (3) prediction of values for kinetic parameters, concentrations, fluxes and global tolerances for each phenotype.We now show that these methods also automate prediction of phenotype-specific mutation rate constants and equilibrium distributions of phenotype diversity in populations undergoing steady-state exponential growth. We introduce this theoretical framework in the context of a case study involving a small molecular system, a primordial circadian clock, compare and contrast this framework with other approaches in theoretical population genetics, and discuss experimental challenges for testing predictions.
Distinct threat and valence signals in rat nucleus accumbens core
Authors: Ray Madelyn H. , Moaddab Mahsa , McDannald Michael A. 
Published: May 2021

AbstractAppropriate responding to threat and reward is essential to survival. The nucleus accumbens core (NAcc) is known to support and organize reward behavior. More recently our laboratory has shown the NAcc is necessary to discriminate cues for threat and safety. To directly reveal NAcc threat responding, we recorded single-unit activity from 7 female rats undergoing Pavlovian fear discrimination. Rats fully discriminated cues for danger, uncertainty, and safety. Demonstrating direct threat responding, most NAcc neurons showed greatest firing changes to danger and uncertainty. Heterogeneity in cue and reward firing led to the detection of multiple, functional populations. One NAcc population specifically decreased firing to threat (danger and uncertainty). A separate population bi-directionally signaled valence through firing decreases to negative valence events (danger and uncertainty) and opposing firing increases to positive valence events (reward and safety onset). The findings point to the NAcc as a neural source of threat information and a more general valence hub.
Unbiased screen of human transcriptome reveals an unexpected role of 3′UTRs in translation initiation
Authors: Yang Yun , Fan Xiaojuan , Ye Yanwen , Chen Chuyun , Ludwig Sebastian E.J. , Zhang Sirui , Lu Qianyun , Will Cindy L. , Urlaub Henning , Sun Jing , Lührmann Reinhard , Wang Zefeng 
Published: May 2021

AbstractAlthough most eukaryotic mRNAs require a 5′-cap for translation initiation, some can also be translated through a poorly studied cap-independent pathway. Here we developed a circRNA-based system and unbiasedly identified more than 10,000 sequences in the human transcriptome that contain Cap-independent Translation Initiators (CiTIs). Surprisingly, most of the identified CiTIs are located in 3′UTRs, which mainly promote translation initiation in mRNAs bearing highly structured 5′UTR. Mechanistically, CiTI recruits several translation initiation factors including eIF3 and DHX29, which in turn unwind 5′UTR structures and facilitate ribosome scanning. Functionally, we showed that the translation of HIF1A mRNA, an endogenous DHX29 target, is antagonistically regulated by its 5′UTR structure and a new 3′-CiTI in response to hypoxia. Therefore, deletion of 3′-CiTI suppresses cell growth in hypoxia and tumor progression in vivo. Collectively, our study uncovers a new regulatory mode for translation where the 3′UTR actively participate in the translation initiation.
Individual expression of hepatitis A virus 3C protease induces ferroptosis in human cellsin vitro
Authors: Komissarov Alexey A. , Karaseva Maria A. , Roschina Marina P. , Shubin Andrey V. , Lunina Nataliya A. , Kostrov Sergey V. , Demidyuk Ilya V. 
Published: May 2021

AbstractRegulated cell death (RCD) is a fundamental process common to nearly all living beings and essential for the development and tissue homeostasis in animals and humans. A wide range of molecules can induce RCD including a number of viral proteolytic enzymes. To date, numerous data indicate that picornaviral 3C proteases can induce RCD. In most reported cases, these proteases induce classical caspase-dependent apoptosis. In contrast, the human hepatitis A virus 3C protease (3Cpro) has recently been shown to cause caspase-independent cell death accompanied by previously undescribed features. Here, we describe the 3Cpro effect on human cellsin vitroand demonstrate that the enzyme induces ferroptosis which is mediated by iron ions and cause oxidative stress in the cells. This is the first demonstration that proteolytic enzyme can induce ferroptosis, the recently discovered and actively studied type of RCD.
Sustainable electronic human resource management systems and firm performance: an empirical study
Authors: Bag Surajit , Dhamija Pavitra , Pretorius Jan Harm Christiaan , Chowdhury Abdul Hannan , Giannakis Mihalis 
Published: May 2021

PurposeThe authors aim to investigate whether ability electronic human resource management (e-HRM) practices, opportunity enhancing e-HRM practices and motivation enhancing e-HRM can possibly lead to development of sustainable e-HRM systems. Finally, the authors also examined if sustainable e-HRM systems can enhance firm performance.Design/methodology/approachThe model was developed using dynamic capability view perspective. The study tests theoretical model and presents findings by analysing data (partial least squares structural equation modelling method) gathered from 151 South African firms.FindingsThe findings indicate that ability enhancing e-HRM practices and motivation enhancing e-HRM practices can result in development of sustainable e-HRM systems, and findings also indicate that sustainable e-HRM systems can improve firm performance.Practical implicationsEmphasis is required on ability enhancing e-HRM practices and motivation enhancing e-HRM practices to develop sustainable e-HRM systems. Once workforce understand the complete benefits of e-HRM, they will start using this system on a regular basis for activities including goal setting, and performance measurement. The development of sustainable e-HRM systems will improve firm performance especially from cost control and customer satisfaction perspective.Originality/valueThis study advances the conceptual debate in the e-HRM domain through the development and testing of theoretical model.
Financial efficiency of commercial banks listed in Egyptian stock exchange using data envelopment analysis
Authors: Kamel Mahmoud Abdelrahman , Mousa Mohamed El-Sayed , Hamdy Randa Mohamed 
Published: May 2021

PurposeThis study used data envelopment analysis (DEA) models to measure financial efficiency of twelve commercial banks listed in the Egyptian stock exchange (CBLSE), along with evaluating changes to the financial efficiency during the period 2017–2019.Design/methodology/approachThe study used BCC-I, cross-efficiency, super-efficiency models, and Malmquist productivity index (MPI) to assess financial efficiency of the examined banks. The available data from both inputs and outputs were analyzed using R. studio V.I.3. 1056 software.FindingsOut of twelve banks examined, only four banks were efficient under BCC-I model over different years of the study period; however, only one bank (CIB) appeared to be the most efficient compared to other peers in the study sample. Moreover, MPI results revealed decreased financial efficiency during the study period, due to the decreased technological innovation, except for HDB. Tobit regression results confirmed that total assets and total equity are significant factors impacted financial efficiency of CBLSE.Practical implicationsThis study sheds light on the importance of evaluating financial efficiency of CBLSE to all stakeholders, to pinpoint weaknesses in banks' performance, and for evaluating financial policies and investment decisions.Originality/valueSeveral studies sought to implement different models of DEA to assess banking performance in different regions of the world, but very few studies examined financial efficiency of banks. To the best of authors’ knowledge, this study is one of those few that addressed financial efficiency of banks in Egypt.
The Airbnb effect on areas subject to urban renewal in Valencia (Spain)
Authors: García-Amaya Alicia María , Temes-Cordovez Rafael , Simancas-Cruz Moisés , Peñarrubia-Zaragoza María Pilar 
Published: May 2021

Purpose
In the past decade, urban tourism has increased worldwide as a result of the development of peer-to-peer (P2P) accommodation platforms such as Airbnb, causing a major disruption to the tourism industry and urban space. The expansion of tourist accommodation in cities has motivated many governments to act, to control its effects and reduce conflicts between tourists and residents. The purpose of this paper is to identify the attractions that have motivated the concentration of P2P accommodation and its effects in specific areas of Valencia different from the historical centre: the Russafa and El Cabanyal-Canyamelar neighbourhoods.

Design/methodology/approach
The methodology used includes fieldwork and spatial analysis of factors such as the housing market, tourist attractions, local businesses and urban renewal policies.

Findings
The current spatial distribution pattern of tourist housing in Valencia is the result of the convergence of various factors: the initial presence of tourists in some areas; the evolution of certain aspects of the neighbourhood due to urban renewal; the concentration of tourist and leisure activities; the effects of the legal framework

Originality/value
Many researchers have addressed the effects of rising short-term rentals (STRs) in cities, but the causes of their concentration in specific neighbourhoods different from historical centres have not yet been sufficiently investigated. This research looks in depth at the urban causes and effects of the spatial distribution of tourist housing in Valencia, to anticipate possible future concentrations of STRs in other areas and to avoid gentrification. The methodology and results could be applied to other cities. The research implies a detailed and analysis of different aspects that act simultaneously such as the housing market, the evolution of the population and changes in the business.
Analysis of Barcelona’s tourist landscape as projected in tourism promotional videos
Authors: Georgescu Paquin Alexandra , Cerdan Schwitzguébel Aurélie 
Published: May 2021

Purpose
The purpose of this paper is to analyze the tourist landscape as represented in Turisme de Barcelona’s YouTube tourism promotional videos, looking at the landscape’s tangible locations, symbolic and tourist assets and the protagonists in an effort to interpret its storytelling in an overtourism context.

Design/methodology/approach
The mixed methodology is based on a visual content analysis of promotional videos posted on the official Barcelona tourism YouTube channel. Quantitative data analysis about the assets and their localization was completed with a qualitative assessment of the way these assets are displayed to unveil the narrative they convey.

Findings
The results highlight that Barcelona’s projected image is mainly based on tangible heritage (especially monuments), its recognizable cityscape and its eno-gastronomic assets. This rather conventional image is geographically concentrated on the neighborhoods perceived as tourist neighborhoods.

Practical implications
This analysis provides a critical reflection of the actual strategy of destination management organizations and the storytelling they transmit. The findings can help to orientate their future actions and provide a method of analysis that can be repeated for other destinations.

Originality/value
This paper sheds new light on the use of urban landscapes in nonstatic images both as a narrative subject and as a tangible tourist space in promotional discourse.
“Let's keep this video as real as possible”: young video bloggers constructing cognitive authority through a health-related information creation process
Authors: Multas Anna-Maija , Hirvonen Noora 
Published: May 2021

PurposeThis study examines the information literacy practices of young video bloggers, focusing on the ways in which they construct their cognitive authority through a health-related information creation process.Design/methodology/approachThis study draws upon socially oriented information literacy research and nexus analysis as its methodological framework. Data, including YouTube videos, theme interviews and video diaries, were collected with three Finnish video bloggers and qualitatively analysed using nexus analytical concepts to describe the central elements of social action.FindingsThe study shows that video bloggers employ several information practices during the information creation process, including planning, information-seeking, organization, editing and presentation of information. They construct their cognitive authority in relation to their anticipated audience by grounding it on different types of information: experience-based, embodied and scientific. Trustworthiness, emphasized with authenticity and genuineness, and competence, based on experience, expertise and second-hand information, were recognized as key components of credibility in this context.Originality/valueThis study increases the understanding of the complex ways in which young people create information on social media and influence their audiences. The study contributes to information literacy research by offering insights into the under-researched area of information creation. It is among the few studies to examine cognitive authority construction in the information creation process. The notion of authority as constructed through trustworthiness and competence and grounded on different types of information, can be taken into account in practice by information professionals and educators when planning information literacy instruction.
Remittances and food security
Authors: Subramaniam Yogeeswari , Masron Tajul Ariffin , Azman Nik Hadiyan Nik 
Published: May 2021

PurposeThe continuous and rapid growth of remittances has become one of the sources of income for millions of poor families in developing countries. As such, an increase of remittance flow can have a significant impact on the ability of the household not only to get enough food but also to get nutritious foods. Therefore, this study investigates the implication of remittances on food security (FS) in 51 developing countries from 2011–2016.Design/methodology/approachA dynamic panel estimator is applied to examine remittances and FS nexus.FindingsBy using the dynamic panel estimator, the results indicate that the level of food supply tends to be higher in countries with a higher flow of remittances. This study justifies the need for high income as well as high middle-income countries to be more open and receptive to migration as this could indirectly the mean through which host countries can assist economic development in low-income developing countries.Originality/valueGiven the diverse measure of FS, past studies demonstrated a positive association between remittance and FS, but it may focus on only one dimension of FS. To the authors’ limited knowledge, this is not enough to know the importance of remittance in determining the overall FS status. Hence, this study wishes to extend the literature by using a more comprehensive measure of FS and more countries in the sample.
Identifying global leadership potential
Authors: Knoll Claudia , Sternad Dietmar 
Published: May 2021

PurposeThis article investigates which criteria and processes are used to identify global leadership potential (GLP) in multinational corporations.Design/methodology/approachFirst, the literature at the intersection between leadership potential and global leadership is reviewed to identify a set of criteria that can be used for assessing GLP. The findings are then validated in a qualitative study against a sample of nine global corporations.FindingsSeveral traits (integrity and resilience), attitudes (learning orientation, motivation to lead, change orientation, drive for results, customer orientation and a global mindset) and competencies (cognitive complexity and intercultural, interpersonal, leadership, learning, change and business competencies) are associated with GLP. The core steps in the GLP identification process are nomination, assessment and confirmation. These steps can be complemented by a preassessment phase and a subsequent talent dialogue.Practical implicationsThe results of this research can inform human resource (HR) management practitioners in their endeavor to successfully identify and assess potential future global leaders.Originality/valuePrior research has focused either on defining global leadership or on assessing leadership potential in general, without a clear focus on identifying global leaders. In this article, the two concepts of global leadership and leadership potential are combined, thus providing an integrated content and process model that indicates how global corporations select their future global leaders.