bluuebunny/crossref_metadata_2025_split huggingface dataset:
SCoT Markers Analysis of Genetic Diversity of Polygonatum, A Medicinal and Edible Plant
Authors: Cao Shi dong , Chen Senmiao , Cao Shuyu , Chen Nipi , Jin Bo 
Published: July 2021

Abstract
Background: As a traditional Chinese medicine, Polygonatum has been demonstrated to have immunomodulatory, antibacterial, anti-inflammatory, anti-aging, anti-cancer, hypoglycemic and other pharmacological effects. However, the germplasm resources of Polygonatum have been destroyed in recent years and the research on its genetic diversity is extremely scarce. In this study, the genetic diversity of 28 Polygonatum germplasms from 11 different provinces in China was evaluated by Start codon targeted (SCoT) marker. Results: A total of 365 bands were generated by 15 SCoT primers, of which 355 were polymorphic, with a high polymorphism of 97.3%. And the genetic similarity coefficient is between 0.59 and 0.75, indicating a high genetic diversity. UPGMA (Unweighted Pair Group Method with Arithmetic Mean) dendrogram, PCoA (Principal Coordinate Analysis) and Structure analysis have similar results in grouping Polygonatum germplasm and they are all divided into two populations. We found that there was a certain correlation between the genetic distance and geographical distance of Polygonatum germplasm. By analyzing other valid genetic diversity parameters (Na, Ne, H, I), it is clarified that Polygonatum has abundant alleles and abundant genetic diversity among populations. Conclusions: This study suggests that Polygonatum germplasm resources from different provenances have rich genetic diversity. The SCoT molecular marker is a valuable marker system and can be used for genetic analysis of Polygonatum resources. This will be helpful to further study the preservation and genetic improvement of Polygonatum germplasm.
Fracking Technique: The Novel Approach To Crack Deep Calcified Plaque In Common Femoral Artery With Hydraulic Pressure.
Authors: Haraguchi Takuya , Fujita Tsutomu , Kashima Yoshifumi , Tsujimoto Masanaga , Takeuchi Tsuyoshi , Tadano Yutaka , Hachinohe Daisuke , Kaneko Umihiko , Kobayashi Ken , Kanno Daitaro , Sato Katsuhiko 
Published: July 2021

Abstract
Background:The patency of conventional peripheral intervention for atherosclerotic lesions in common femoral artery (CFA), called “no stenting zone”, are not superior to surgical endarterectomy due to calcified plaque occupying the area. Plaque modification strategies to obtain acute gain in CFA provide the better clinical outcome compared to standard balloon angioplasty. Atherectomy devices, which focus on modification of superficial calcification, contribute to the improvement of clinical outcomes. However, deep calcium resists vessel expansion so that luminal gain is not easily achieved. Main text:We propose a novel calcified plaque modification technique, named the” Fracking technique”. The term fracking refers to how a rock is fractured apart by the high hydraulic pressure. This technique is to crack deep calcification with hydraulic pressure with a balloon indeflator through 18-gauge needle, which punctures into calcification in order to obtain larger acute luminal gain. Case 1 involved an 81-year-old male with eccentric calcified plaque in right CFA. Conventional balloon angioplasty for the lesion provided the suboptimal minimal lumen area (MLA) from 6.2 to 10.7mm2 with intravascular ultrasound (IVUS) measurement. Fracking technique was implemented to obtain the larger MLA. After fracking was repeated three times until there were no more Fracking points, a much larger MLA of 27.1mm2 was achieved without complications. Case 2 involved a 72-year-old male undergoing hemodialysis presented with ischemic rest pain of his right limbs due to severe stenosis with eccentric calcification in distal CFA to proximal superficial femoral artery. MLAs with IVUS before and after ballooning were 10.0mm2, 13.1mm2, respectively. None of the results of MLA lived up to our expectation. Fracking technique was attempted, and MLA of 28.9mm2 was successfully obtained after this technique. No complications observed. Conclusions:Fracking technique was effective for severe calcified lesion in CFA to obtain significantly larger lumen area which will lead to long-term patency superior to conventional peripheral intervention.
Analysis of NGS-Ministr as A New Detection Strategy Chromosomal Trisomy 16 of Fetal Materials in Miscarriages
Authors: Yao Lilan , Tian Yafei , Zhu Yijian , Lu Daru , Chen Linbo , Mou Li , Yin Rui , Luo Li , Chai Siyu , Yang Jingmin , Chen Pengyu 
Published: July 2021

Abstract
Background: Although many technologies can identify chromosomal abnormalities, they can’t completely replace the advantages of short tandem repeat (STR) genotyping technology. The miniSTR typing based on next generation sequencing (NGS-miniSTR) with high throughput, accuracy and sensitivity has the potential to determine the trisomy and overcome the limitations of conventional STR techniques, which never be tried. The study aimed to explore the value of the NGS-miniSTR in trisomy testing, which is an extension and improvement of the methodology of chromosomal detection technology.Methods: A total of 140 fetal materials in miscarriages (70 trisomy 16 and 70 normal karyotypes) screened by copy-number variation sequencing (CNV-seq) were genotyped using the customed panel containing 11 miniSTRs based on NGS. Direct counting method, chi-square (χ2) test, k-means clustering analysis and Mahalanobis distance were operated to compare the difference of miniSTR between trisomy 16 and normal group, and then analyze whether trisomy 16 could be identified.Results: All miniSTR results based on NGS were successfully obtained, except for D16S771 in 21 samples. There were significant differences in the average depth of coverage at each locus in each sample. Direct counting method and chi-square (χ2) test showed significant differences in allelic pattern and read ratio between trisomy and normal group, respectively. Almost all samples correctly divided into 2 clusters based on diallelic STR reads ratios according to k-means clustering analysis. In addition, the Mahalanobis distance showed that D16S771 had multiple outliers. Conclusion: A new strategy of miniSTR-NGS was firstly introduced to successfully detect all samples of trisomy 16 in the fetal material in miscarriages, which revealed that the allelic pattern and allelic read ratio could be effective indexes to identify the number of chromosomes.
High Resistance Mutation To cART In HIV-1 Exposed Infected Children And Recent Emergence Of CRF02_AG Variant In Bouar, A Rural Environment of Central African Republic
Authors: Vickos Ulrich , Gaiera Giovanni , Cotugno Nicola , Gadia Christelle Luce Bobossi , Demi Ornella Anne Sibiro , Sala Angelo , Sampaolo Michele , Faou Alain Le , Boeri Enzo 
Published: July 2021

Abstract
Introduction: The emergence of HIV-1 recombinant forms and Drug combined antiretroviral therapy (cART) resistance are frequent in the therapeutic course of HIV-infected children in Low and Middle-Income Countries (LMIC) precisely in Central African Republic (CAR) as evidenced by studies carried out in the Bangui capital. Vertical transmission rate including during breastfeeding is 12.4. The aim of study is to analyze retrospectively the molecular characterization of sequencing results and mutation detected in HIV infected children who have received cART initiated since infancy. Methods: The 2019 retrospective review of the clinical, therapeutical, and immunological-molecular records of six children who were performed the genome sequencing, followed in Bouar, at the St Michel IST and HIV Center, in the north-west of the CAR. These children infected with HIV perinatally had their seropositive test performed at a median age of 6 years and initiated cARTs at an average age of 7 years as part of treatment regimens also used for the prevention of vertical transmission and the initiation of treatment for HIV infection in CAR. Results: We analyzed results from viral RNA extracted amplification and sequencing of 6 children plasma samples collected under first line antiretroviral therapy. Persistent opportunist infections confirmed Immunosuppression in all patients. Sequencing of viral genomes revealed high level resistance mutations to NRTIs (ABC, FTC and 3TC) in five patients and to NNRTIs (EFV, NVP used locally and DOR, ETR and RPV unused) for all with ambiguous positions in amino-acids comparison and deletion. The HIV-1 group M found in these patients were sub-type A (1) and G-J (1), and CRF02_AG (4), respectively. Three CRF02_AG strains formed a variant cluster by strongly detaching from other CAR and worldwide strains with robust boostrap at 91. Retention and adherence were complicated by the cART limited number and laboratory tests, the irregular supply, and the remoteness of patients from the Center. Conclusions: The genomes sequencing showed that resistance mutations made the treatment inefficient confirming the observed virological and immunological failure. The CRF02_AG genotype is an emerging variant, probably of foreign origin. This discovery clearly highlights the importance and the necessity of ART genetic resistance testing and personalized medicine.
Efficacy of Shear Wave Elasticity for Evaluating Myocardial Hypertrophy in Hypertensive Rats
Authors: Takaya Yoichi , Nakamura Kazufumi , Nakayama Rie , Hiroaki Ohtsuka , Amioka Naofumi , Kondo Megumi , Akazawa Kaoru , Ohno Yuko , Ichikawa Keishi , Saito Yukihiro , Akagi Satoshi , Yoshida Masashi , Miyoshi Toru , Ito Hiroshi 
Published: July 2021

Abstract
Shear wave (SW) imaging is a novel ultrasound-based technique for assessing tissue characteristics. SW elasticity may be useful to assess the severity of hypertensive left ventricular (LV) hypertrophy. This study aimed to evaluate the efficacy of SW elasticity for assessing the degree of myocardial hypertrophy using hypertensive rats. Rats were divided into hypertension group and control group. SW elasticity was measured on the excised heart. Myocardial hypertrophy was assessed histologically. LV weight was greater in hypertension group. An increase in interventricular septum and LV free wall thicknesses was observed in hypertension group. SW elasticity was significantly higher in hypertension group than in control group (14.6 ± 4.3 kPa vs. 6.5 ± 1.1 kPa, P < 0.01). The cross-sectional area of cardiomyocytes was larger in hypertension group than in control group (397 ± 50 µm2 vs. 243 ± 14 µm2, P < 0.01), and SW elasticity was positively correlated with the cross-sectional area of cardiomyocytes (R = 0.96, P < 0.01). This study showed that SW elasticity was higher in hypertensive rats and was closely correlated with the degree of myocardial hypertrophy, suggesting the efficacy of SW elasticity for estimating the severity of hypertensive LV hypertrophy.
Development and Validation of An Elderly Care Capacity Needs Questionnaire in Elderly Care Institutions with the Model of Combination of Medical and Health Care
Authors: Zhou Liping , Yang Yunfan , Xiao Xiao , Tang Siyuan , Su Yuhua 
Published: July 2021

Abstract
Background

China's elderly population is growing rapidly. As the primary provider of geriatric care services, the expertise and care competencies of nursing staff directly affect the quality of services provided by elderly care institutions. To develop a geriatric care capacity needs questionnaire to provide an assessment tool for the implementation of geriatric care quality improvement and staff capacity building programs in elderly care institutions.
Methods

Based on the literature review, a questionnaire item pool was established. The initial questionnaire was formed by interviewing senior nursing staff and a pilot survey. The formal questionnaire was formed by testing the reliability and validity of the questionnaire.
Results

The Elderly Care Capacity Needs Questionnaire (ECCNQ) was composed of 12 dimensions. The Cronbach's Alpha coefficient of the total questionnaire was 0.860. The Spearman-Brown coefficient was 0.832, Guttman split-half coefficient was 0.83. The correlation coefficient between each dimension and the total questionnaire was 0.472–0.737.
Conclusions

The reliability and validity of the ECCNQ are acceptable, with good stability and effectiveness, which can be used as an evaluation tool for elderly care capacity needs.
Vicarious Ratings of Social Touch, the Effect of Age and Autistic Traits
Authors: Haggarty Connor , Moore David , Trotter Paula , Hagan Rachel , McGlone Francis , Walker Susannah 
Published: July 2021

Abstract
Tactile sensitivities are common in Autism Spectrum Disorders (ASD). Psychophysically, slow, gentle stroking touch is typically rated as more pleasant than faster or slower touch. Vicarious ratings of social touch results in a similar pattern of velocity dependent hedonic ratings as directly felt touch. Here we investigated whether adults and children’s vicarious ratings vary according to ASD diagnosis and self-reported autistic traits. Adults’ scoring high on the AQ rated stroking touch on the palm as less pleasant than a Low AQ group. However, in contrast to our hypothesis, we did not find any effect of ASD diagnosis on children’s touch ratings despite parental reports highlighting significant somatosensory sensitivities. These results are discussed in terms of underpinning sensory and cognitive factors.
Prophylactic Bilateral Central Neck Dissection Should Be Evaluated Based on Prospective Study of 581 PTC Patients
Authors: YAN Shouyi , Yu Jiafan , zhao wenxin , WANG Bo , ZHANG Liyong 
Published: July 2021

Abstract
Background: Prophylactic central lymph node dissection (PCND) had been a basic consensus for patients with papillary thyroid carcinoma in China. However, unilateral or bilateral central lymph node dissection (CND)was still controversial. This study aimed at investigating the safety and long-term benefit for the patients with bilateral central lymph node dissection (BCCD). Methods: 581 patients were enrolled and divided randomly into the test and control groups according to a different range of CND. 285 patients were prospectively assigned to undergo lobe thyroidectomy plus BCND in the test group, in comparison 296 patients were assigned to undergo lobe thyroidectomy plus ipsilateral central lymph node dissection (ICND) in the control group. Results: We found that the numbers of total LN and N1a in the test group were higher than that of the control group (p=0.002), but there was no difference in the number of metastasized lymph nodes (p=0.857) and tumor recurrence (p=0.308). Additionally, in the aspect of postoperative complication (1 day after surgery), the serum levels of parathyroid hormone in the BCND group were lower than that in the ICND group (P =0.010), and the numbers of transient laryngeal nerve palsy were higher than that(p=0.033). meanwhile we further found that tumors size larger than 1cm and tumor side lymph node metastasis were related to the contralateral lymph node metastasis. Conclusion: BCND resulted in more positive lymph nodes and complications while did not change the patient's long-term prognosis. It may be an alternative for patients with tumor sizes larger than 1cm in large medical centers.
A Network Perspective on Marital Satisfaction among Older Couples
Authors: Li Fengzhan , Ren Lei , Wang Xiuchao , Jin Yinchuan , Ma Zhujing , Yang Qun , Wang Dahua 
Published: July 2021

Abstract
BackgroundAs the world’s population is getting older, it will be helpful for older people to maintain good mental health by targeting their marital satisfaction. The present study aims to investigate how the concepts of marital satisfaction are related to each other and reveal the key concepts for older people. MethodsFour hundred ninety-four older people (247 couples aged 68.19±5.45) participated in the study. Marital satisfaction was assessed by the 10-item marital satisfaction subscale of the ENRICH (Evaluation and Nurturing Relationship Issues, Communication and Happiness) scale. We used network analysis to estimate the network structure of these 10 items and calculated the strength centrality for each item. ResultsThe results showed that all items were positively associated in the final network, and the whole network was relatively high-connected. Four edges with the strongest regularized partial correlations appeared between “leisure activities and spending time together” and “emotional expression” (r=0.33), “personality and habits” and “communication and understanding” (r=0.30), “economic status and the manner of determining economic affairs” and “relationship with relatives and friends” (r=0.24), and “make decisions and resolve conflict” and “emotional expression” (r=0.22). “Emotional expression” was the most connected node in the network. “Communication and understanding” and “views are consistent” had the second and third highest node strength values, respectively. Thus, it will be the best way to promote marital satisfaction by prioritizing the improvement of “emotional expression” for older couples.Conclusions﻿The present study offers a new perspective on the marital satisfaction of older couples using network analysis for the first time. It carefully explores several links among specific concepts of the whole network and found it was relatively high-connected. “Emotional expression” is the central concept in marital satisfaction. “Communication and understanding” and “views are consistent” have the second and third highest node strength values in the network. These results might provide potential targets for the improvement of marital satisfaction among older couples.
Effectiveness of Patient-centered Care for Diabetes Self-management to Improve Glycemic Control and Self-care Behaviors in Adults With Type 2 Diabetes: a Systematic Review
Authors: Asmat Kainat , Dhamani Khairunnisa , Gul Raisa , Froelicher Erika Sivarajan 
Published: July 2021

Abstract
Background: Patient-centered care in diabetes self-management might be a significant factor in improving self-care outcomes yet the supporting evidence is inadequate. This review is aimed to assess the effectiveness of patient-centered self-management care interventions on self-care outcomes such as glycemic control (HbA1c) and self-care behaviors in adults with type-2 diabetes compared with usual care. Methods: CINAHL, PubMed, Cochrane Library, Google Scholar and the HEC Pakistan digital library were searched for English language studies that assessed patient-centered self-management educational and/or behavioral interventions in adults aged 18 years or above with type 2 diabetes from 1991 to 2020. Interventional studies comprising randomized controlled trials (RCT) and quasi experimental studies (QES) with at least three months follow up and reporting on self-care outcomes with glycemic control (HbA1c) as primary outcome and self-care behaviors including diet control, physical activity, medication adherence and foot care as secondary outcomes were included. Results: Of the 168 identified records, 25 were found eligible comprising 21 RCTs and 4 QESs with total 4,443 participants. The meta-analysis involved 23 studies that provided enough information for a pooled estimate of HbA1c. Compared with the control group, patient-centered self-management interventions significantly lowered HbA1c −0.53 (95% CI −0.73, −0.32). Stratified analysis for HbA1c with respect to various aspects of intervention showed larger effects in interventions employing both educational and behavioral components −0.59 (95% CI −0.86, −0.32), spanned over shorter (<03 months) duration −0.56 (95% CI −0.86, −0.27), administered by nurses −0.80 (95% CI −1.44, −0.16) and delivered in community setting −0.65 (95% CI −1.00, −0.29). Moreover, patient-centered self-management interventions were found effective in improving diet control, physical activity and foot care. Conclusion: This systematic review provided the evidence supporting the effectiveness of patient-centered self-management care interventions in improving glycemic control and self-care behaviors in adults with type 2 diabetes and identified key features of intervention contributing towards success.
Periodic Heat Stress Licenses EMSCs Differentiation Into Osteoblasts via YAP Signaling Pathway Activation
Authors: shi wentao , wang zhe , Bian Lu , wu yiqing , mei huiya , zhou yanjun , Zhang Zhijian , Wang Qing , Zhao Peng , Lu Xiaojie 
Published: July 2021

Abstract
BackgroundThe repair and regeneration of large bone defects represent a highly challenging task for bone tissue engineering. Although recent studies have shown that osteogenesis is stimulated by periodic heat stress, the thermal regulation of the osteogenic differentiation of ectomesenchymal stem cells (EMSCs) is not well studied. Methods and resultsIn this study, the direct effects of periodic heat stress on the differentiation of EMSCs into osteoblasts were investigated. EMSCs derived from rat nasal respiratory mucosa were seeded in culture plates, followed by 1 h of heat stress at 41 °C every 7 days during osteogenic differentiation. Based on the results of the present study, periodic heating increases alkaline phosphatase (ALP) activity, upregulates osteogenic-related proteins and promotes EMSC mineralization. In particular, increased YAP nuclear translocation and YAP knockdown inhibited osteogenic differentiation induced by heat stress. Furthermore, the expression and activity of transglutaminase 2 (TG2) were significantly increased after YAP nuclear translocation. Conclusion﻿Together, these results indicate that YAP plays a key role in regulating cellular proteostasis under stressful cellular conditions by modulating the TG2 response.
Evidence of origin with epigenetic change of metachronous lesions in early gastric cancer after endoscopic submucosal dissection
Authors: Kubota Yo , Tanabe Satoshi , Azuma Mizutomo , Horio Kazue , Fujiyama Yoshiki , Soeno Takafumi , Furue Yasuaki , Wada Takuya , Watanabe Akinori , Ishido Kenji , Katada Chikatoshi , Yamashita Keishi , Koizumi Wasaburo 
Published: July 2021

Abstract
Early gastric cancer (EGC) with metachronous lesions developing on scars after endoscopic submucosal dissection (ESD) is extremely rare and hard to treat. We evaluated whether DNA methylation of the cancer-specific methylation gene, cysteine dioxygenase type 1 (CDO1), would predict such lesions. CDO1 methylation (TaqMeth) values were compared between 11 patients with metachronous lesions developing on scars after ESD (M group) identified from 2,055 patients (0.5%) and 33 patients with EGC with no confirmed evidence of metachronous lesions at > 3 years after ESD (solitary [S] group). To assess Helicobacter pylori influence, 11 H. pylori-negative EGC patients (N group) were also analyzed. Each ESD specimen was measured at the tumor (T) and 4-points separated tumor-adjacent noncancerous mucosa (TAM). TaqMeth values for T were significantly higher than TAM (S + M) (P = 0.0019) and TAM (N) (P < 0.0001). Moreover, TAM (M) had significantly higher TaqMeth values than TAM (S) (P < 0.0001) suggesting that TAM (M) exhibited CDO1 hypermethylation similar to T (P = 0.5713). Additionally, TaqMeth values for TAM (S) were significantly higher than TAM (N) (P < 0.0001). The receiver operating characteristic for discriminating the highest TaqMeth values separated TAMs (M) from those of TAMs (S) was 0.81. CDO1 hypermethylation promisingly predicted EGC with metachronous lesions developing on scars after ESD.