bluuebunny/crossref_metadata_2025_split huggingface dataset:
Phase II Study of Neoadjuvant Chemotherapy With S-1 Plus Oxaliplatin for Gastric Cancer Clinical T4 or N2-3
Authors: SERIZAWA AKIKO , Kuramochi Hidekazu , Taniguchi Kiyoaki , Ota Masaho , Katagiri Satoshi , Yamada Takuji , Kotake Sho , Ito Shunichi , Suzuki Kazuomi , Yamamoto Masakazu 
Published: April 2021

Abstract
Background: In Japan, the standard treatment for stage II or III gastric cancer is D2 gastrectomy followed by administration of S-1 for one year. However, patients with stage III disease have unsatisfactory survival rates. The purpose of this study was to evaluate the efficacy and safety of neoadjuvant chemotherapy consisting of S-1 and oxaliplatin for advanced gastric cancer.Methods: Patients with cT4 or cN2–3 gastric cancer were scheduled to receive two courses of chemotherapy (130 mg/m2 oxaliplatin on Day 1, 80 mg/m2 S-1 per day twice daily for 14 days) followed by surgery. The primary endpoint was the R0 resection rate. The secondary endpoints were rates of completion of protocol treatment, pathological response, and adverse events; and 3-year overall survival, 5-year overall survival, and 5-year recurrence-free survival.Results: Between May 2016 and March 2019, 30 patients were enrolled in the study, all of whom completed the protocol treatment. The R0 resection rate (primary endpoint) was 93.3% (95% confidence interval: 77.9–99.2). The pathological response rate was 63.3%. Grade 3–4 toxicities included anemia (3.3%), anorexia (6.7%), and fatigue (3.3%). Relative dose intensities were 91.2% and 94.2% for S-1 and oxaliplatin, respectively.Conclusions: Neoadjuvant S-1 and oxaliplatin is highly effective, achieving an acceptable R0 resection rate with relatively few severe toxicities and good compliance. Trial registration information:Registry name: A prospective intervention study on the availability of preoperative SOX therapy for T4 or N2-3 gastric cancerTrial ID: UMIN: UMIN000024656URL: https://upload.umin.ac.jp/cgi-open-bin/ctr/ctr_view.cgi?recptno=R000028365
Association between metabolic associated fatty liver disease and Osteoarthritis using data from the Korean National Health and Nutrition Examination Survey (KNHANES)
Authors: Han A lum 
Published: April 2021

Abstract
Osteoarthritis of the knee (knee OA) is on the rise due to the aging population and increasing obesity. In addition to mechanical stress attributed to weight and age, osteoarthritis is associated with obesity and metabolic dysregulation. Here, a cross-sectional study targeting retrospectively registered 17,476 adults aged 50 years or older who were enrolled in the National Health and Nutrition Survey (2010–2011) was performed to analyze the association between the newly named metabolic associated fatty liver disease (MAFLD) and knee OA. Fatty liver index (FLI) ≥ 60 confirmed the presence of MAFLD, and FLI < 30 indicated the absence of MAFLD. Knee OA was diagnosed according to the Kellgren–Lawrence scale based on knee radiography results. A complex sample logistic regression analysis was performed. Statistically significant factors were adjusted to estimate probability ratios, and 95% confidence intervals were used to investigate the association between knee OA and MAFLD. The probability of knee OA was 1.479 times higher in the presence of MAFLD than that in the normal group. The results indicate that MAFLD is significantly associated with knee OA, suggesting that these two disorders should be managed simultaneously.
Conversion of Lignocellulosic Waste Into Effective Flocculants: Synthesis, Characterization, and Performance
Authors: Jahedi Elham , Panahi Reza 
Published: April 2021

Abstract
Development of cationic flocculants from lignocellulosic wastes not only eliminates the health and environmental concerns associated with the use of conventional chemicals but also is the way of waste valorization. In the present study, cellulose fibers extracted from rice husk were cationized through an optimization method based on Response Surface Methodology. The fibers cationized at the optimal conditions had a zeta potential of 15.2±1.0 mV, which was 1.73-fold higher than that of the preliminary experiments. FTIR analysis proved the presence of the corresponding functional groups. The functionalized fibers were biodegradable and had absolutely positive surface charges at a broad pH range. The cationized fibers were employed as a flocculant to remove turbidity from the synthetic wastewaters at various pHs and initial turbidities. The cationic fibers showed the excellent turbidity removals up to 98.5% from the synthetic wastewater without the need for conventional coagulants. In contrast to traditionally cationized fibers, the synthesized flocculants did not affect the effluent color during coagulation-flocculation. The charge neutralization and bridging through adsorption were the governing mechanisms of flocculation. The procedure can be applied on lignocellulosic wastes to develop cationic fibers with the excellent flocculation ability and suitable operational characteristics.
Multi-omics molecular phenotyping of childhood acute lymphoblastic leukemia cell lines reveals novel drug vulnerabilities
Authors: Stahl Matthias , Aswad Luay , Leo Isabelle , Kunold Elena , Post Frederik , Erkers Tom , Struyf Nona , Mermelekas Georgios , Joshi Rubin , Gracia-Villacampa Eva , Östling Päivi , Kalloniemi Olli , Tamm Katja Pokrovskaja , Siavelis Ioannis , Lehtiö Janne , Vesterlund Mattias , Jafari Rozbeh 
Published: April 2021

Abstract
Acute lymphoblastic leukemia (ALL) is the most common childhood cancer. Although standard-of-care chemotherapeutics are sufficient for most ALL cases, there are subsets of patients with poor response who relapse in disease. The biology underlying differences between subtypes and their response to therapy has only partially been explained by genetic and transcriptomic profiling. To characterize ALL subtypes and identify novel pharmacologic vulnerabilities, we performed comprehensive multi-omic analyses of 49 widely-used childhood ALL cell lines, using proteomics, transcriptomics, and pharmacoproteomic characterization. This enabled us to characterize the functional impact of genetic fusions and cellular differentiation states. The proteomics data revealed differences in spliceosome and p53 levels not evident in the transcriptomics data and with improved correlation to drug sensitivity. Focusing on BCP-ALL cell lines, we connected the genotype, molecular phenotype, and functional phenotype with drug response data on 528 oncology drugs. Here, we identified the DAG-analog Bryostatin-1 as a novel therapeutic candidate in the MEF2D-HNRNPUL1 fusion high-risk subtype, for which this drug activated pro-apoptotic ERK signaling associated with molecular mediators of pre-B cell negative selection. Our data also forms an interactive online resource with navigable proteomics, transcriptomics, and drug sensitivity profiles at https://lehtio-lab.se/forall/.
Free energy landscape with two barriers and a transient intermediate state determining the unfolding and folding dynamics of cold shock protein
Authors: Hong Haiyan , Guo Zilong , Sun Hao , Yu Ping , Su Huanhuan , Ma Xuening , Chen Hu 
Published: April 2021

Abstract
Cold shock protein (Csp) is a typical two-state folding model protein which has been widely studied by biochemistry and single molecule techniques. Recently two-state property of Csp was confirmed by atomic force microscopy (AFM) through direct pulling measurement, while several long-lifetime intermediate states were found by force-clamp AFM. We systematically studied force-dependent folding and unfolding dynamics of Csp using magnetic tweezers with intrinsic constant force capability. We found that Csp mostly folds and unfolds with a single step over force range from 5 pN to 50 pN, and the unfolding rates show different force sensitivities at forces below and above ~ 8 pN, which determines a free energy landscape with two barriers and a transient intermediate between them along one transition pathway. Our results provide a new insight on protein folding mechanism of two-state proteins.
Ppe.XapF: High Throughput KASP Assays to Identify Fruit Response to Xanthomonas Arboricola pv. pruni (Xap) in Peach
Authors: Fleming Margaret B. , Miller Texanna , Fu Wanfang , Li Zhigang , Gasic Ksenija , Saski Christopher 
Published: April 2021

Abstract
Background
Bacterial spot, caused by Xanthomonas arboricola pv. pruni (Xap), is a serious peach disease with symptoms that traverse severe defoliation and black surface pitting, cracking or blemishes on peach fruit with global economic impacts. A management option for control and meeting consumer demand for chemical-free, environmentally friendly fruit production is the development of resistant or tolerant cultivars.
Results
We developed simple, accurate, and efficient Ppe.XapF DNA tests based on SNP genotyping with KASP technology to quickly test for bacterial spot resistance alleles in peach fruit and cull at the greenhouse stage. The objective of this research was to validate newly developed Ppe.XapF DNA tests that target the two major QTLs for fruit resistance in peach with diagnostic utility in predicting fruit response to bacterial spot infection.
Conclusion
Our study confirms that only two Ppe.XapF DNA tests, Ppe.XapF1-1 and Ppe.XapF6-2, are needed to distinguish between susceptible and resistant haplotypes. Use of these efficient and accurate Ppe.XapF KASP tests resulted in 44% reduction in seedling planting rate in the Clemson University peach breeding program.
Expression of cardiac copper chaperone encoding genes and their correlation with cardiac function parameters in goats
Authors: Mandour Ahmed S. , Mahmoud Ahmed E. , Ali Asmaa O. , Matsuura Katsuhiro , Samir Haney , Abdelmageed Hend A. , Ma Danfu , Yoshida Tomohiko , Hamabe Lina , Uemura Akiko , Watanabe Gen , Tanaka Ryou 
Published: April 2021

Abstract
The expression of copper chaperone (Cu-Ch) encoding genes enables an in-depth understanding of copper-associated disorders, but no previous studies have been undertaken to highlight Cu-Ch disturbances in heart tissue in ruminants after copper deficiency (CuD). The current study aimed to investigate the Cu-Ch mRNA expression in goat’s heart after experimental CuD and highlight their relationship with the cardiac measurements. Eleven male goats were enrolled and divided into the control group (n = 4) and CuD group (treatment, n = 7), which received copper-reducing dietary regimes (molybdenum and sulfur). Heart function was evaluated by electrocardiography and echocardiography, and at the end of the experiment, all animals were sacrificed, and the cardiac tissues were collected for quantitative mRNA expression by real-time PCR. In the treatment group, cardiac measurements revealed increased preload and the existence of cardiac dilatation and significant cardiac tissue damage by histopathology. Also, the relative mRNA expression of Cu-Ch encoding genes; ATP7A, CTr1, LOX, COX17, as well as ceruloplasmin (CP), troponin I3 (TNNI3), glutathione peroxidase (GPX1), and matrix metalloprotease inhibitor (MMPI1) genes were significantly down-regulated in CuD group. Besides, there was a variable significant correlation between investigated genes and cardiac measurements; meanwhile, a significant inverse correlation was observed between histopathological score and ATP7B, CTr1, LOX, and COX17. In conclusion, this study revealed that CuD induces cardiac dilatation and alters the mRNA expression of Cu-Ch genes, in addition to TNNI3, GPX1, and MMPI1 that are considered key factors in clinically undetectable CuD-induced cardiac damage in goats which necessitate further studies for feasibility as biomarkers.
Design, Synthesis and Apoptosis Inducing Activity of Non-steroidal Flavone- methanesulfonate Derivatives on MCF-7 cell line as Potential Sulfatase Inhibitor
Authors: Javadi Mahdiyeh HS , Iraji Aida , safavi maliheh , Montazeri Hamed , Tarighi Parastoo , Eftekhari Samane , Navidpour Lateifeh , Mirfazli Seyedeh Sara 
Published: April 2021

Abstract
In recent years, focusing on new potent anticancer agents with selective activity is one of the greatest challenges in cancer therapy. Breast cancer is the most common cancer and the main cause of cancer deaths in women. The sulfatase enzyme plays an important role in converting the sulfated steroids into non-sulfate steroid hormones, which increases the growth and development of many hormone-dependent cancers, such as breast cancer. In this regard, structure-based optimization was conducted to design novel flavone-sulfonates pharmacophore as a new steroid sulfatase inhibitor. In the present work, the conventional methods for the synthesis of 4-oxo-2-phenyl-4H-chromen-7-yl methanesulfonate derivatives were reported. Their cytotoxicity was evaluated with MTT assay against a breast cancer cell line (MCF-7). The apoptosis inducing activity of the most cytotoxic compound 3c with an IC50 value of 0.615 µM was evaluated in comparison to docetaxel in the presence of estradiol which is a crucial growth factor to survive the cancerous cells. The results of double staining Annexin V-FITC/PI analysis suggested that the cytotoxic activity of this compound 3c in MCF-7 cells occurs via apoptosis. Molecular docking studies were conducted to clarify the inhibition mode of the most promising compound (3c) over the sulfatase (1P49) binding site. The analysis revealed the role of hydrogen bond interaction with Gly181 and hydrophobic interactions through the 1P49 active site in the ligand-receptor complex as significant descriptors to rationalize the potential inhibition activity.
Enzymatic Hydrolysis of Lignocellulosic Biomass Using an Optimized Enzymatic Cocktail Prepared From Secretomes of Filamentous Fungi Isolated From Amazonian Biodiversity
Authors: Rosa Pamella Santa , de Oliveira Jessica Batista , Filho Spartaco Astolfi , Pereira Nei 
Published: April 2021

Abstract
The use of lignocellulosic biomass (LCB) has emerged as one of the main strategies for generating renewable biofuels. For the efficient use of such feedstock, pretreatments are essential. The hydrolysis of cellulose – major component of LCB - demands enzymatic cocktails with improved efficiency to generate fermentable sugars. In this scenario, lignocellulolytic fungi have enormous potential for the development of efficient enzyme platforms. In this study, two enzymatic cocktails were developed for hydrolysis of two lignocellulosic biomasses: industrial cellulose pulp and cassava peel. The solid biomass ratio in relation to the protein content of the enzyme cocktail were performed by experimental design. The optimized cocktail for the hydrolysis of cellulose pulp (AMZ 1) was composed, in protein base, by 43% of Aspergillus sp LMI03 enzyme extract and 57% of T. reesei QM9414, while the optimal enzyme cocktail for cassava peel hydrolysis (AMZ 2) was composed by 50% of Aspergillus sp LMI03 enzyme extract, 25% of the extract of P. citrinum LMI01 and 25% of T. reesei. The ratio between solids and protein loading for AMZ 1 cocktail performance was 52 g/L solids and 30mg protein/g solids, resulting in a hydrolytic efficiency of 93%. For the AMZ 2 cocktail, the hydrolytic efficiency was 78% for an optimized ratio of 78g/L solids and 19mg protein/g solids. These results indicate that cocktails formulated with enzymatic extracts of P. citrinum LMI01, Aspergillus sp LMI03 and T. reesei QM9414 are excellent alternatives for efficient hydrolysis of plant biomass and for other processes that depend on biocatalysis.
Intracardiac Masses I-Mass Study Single Center Experience Within 12 Years
Authors: Bugra Zehra , Emet Samim , Umman Berrin , Ozer Pelin Karaca , Sezer Murat , Baykiz Derya , Atilgan Dursun , Tireli Emin , Dursun Memduh , Yılmazbayhan Dilek , Karaayvaz Ekrem Bilal , Elitok Ali , Bilge Ahmet Kaya , Goren Taner , Umman Sabahattin 
Published: April 2021

Abstract
Objective : The aim of this cross-sectional, retrospective, descriptive study was to review and classify cardiac masses systematically and to determine their frequencies.Methods : The medical records of 64,862 consecutive patients were investigated within 12 years. Every patient with a cardiac mass imaged by transthoracic echocardiography (TTE) and confirmed with an advanced imaging modality such as transesophageal echocardiography (TEE), computed tomography (CT) and / or cardiac magnetic resonance imaging (CMR) was included. Acute coronary syndromes triggering thrombus formation, vegetations, intracardiac device and catheter related thrombi were excluded.Results : Data demonstrated 127 (0.195 %) intracardiac masses consisting of 33 (0.050 %) primary benign, 3 (0.004 %) primary malignant, 20 (0.030 %) secondary tumors, 3 (0.004 %) hydatid cysts and 68 (0.104 %) thrombi respectively. The majority of primary cardiac tumors were benign (91.67 %), predominantly myxomas (78.79 %), and the less malignant (8.33 %). Secondary cardiac tumors were common than the primary malignant tumors (20:3), with male dominancy (55 %), lymphoma and lung cancers were the most frequent. Intracardiac thrombi was the majority of the cardiac masses, thrombi accompanying malignancies were in the first range (n=17, 25%), followed by autoimmune diseases (n=13, 19.12 %) and ischemic heart disease with low ejection fraction (n=12, 17.65 %).Conclusion: This retrospective analysis identified 127 patients with cardiac masses. The majority of benign tumors were myxoma, the most common tumors that metastasized to the heart were lymphoma and lung cancers, and the thrombi associated with malignancies and autoimmune diseases were the most frequent.
Improved flood forecasting using geomorphic unit hydrograph based on spatially distributed velocity field
Authors: Wang Wen-chuan , Zhao Yan-wei , Chau Kwok-wing , Xu Dong-mei , Liu Chang-jun 
Published: April 2021

Abstract
This paper presents an energy model for determining the overland flow velocity in order to improve the low accuracy problem in flow concentration simulation. It furnishes a novel idea for studying flow concentration in ungauged basins. The model can be widely applied in analysis of spatial velocity field, extraction of instantaneous geomorphic unit hydrograph and development of distributed hydrological model. A distributed flood-forecasting model is constructed for Lianyuan Basin in Hunan Province of China. In the proposed method, gravitational potential energy is transformed into kinetic energy via an analysis of energy distribution of water particles in the basin. Based on the kinetic energy equation, the overland flow velocity simulating the geomorphic unit hydrograph is computed. Rainfall-runoff simulation is then performed by integrating with runoff yield and concentration model. Results indicate that the model based on energy conversion leads to more accurate results. The model has the following advantages: firstly, the spatial distribution of the velocity field is appropriate; secondly, the model has only one parameter, which is easily determined; and finally, flow velocity results can be used for the computation of river network flow concentration.
The Use of Cinchona Alkaloid Derivatives as Chiral Ligands and Organocatalysts
in Asymmetric Catalysis
Authors: Li Minghua , He Wei , Zhang Sheng-Yong 
Published: April 2021

:
Cinchona alkaloids are natural products extracted from cinchona plants, which contain quinine,
quinidine, cinchonine, and cinchonidine. During the past 30 years, due to their attractive structural
properties, these compounds have been used as chiral skeletons and organocatalysts in asymmetric organic
synthesis. In this review, we summarize the applications of cinchona alkaloids derivatives in
asymmetric catalysis recently developed by our group, including oxidation, reduction, and C-C bonds
formation. These studies have solved some of the problems with the various asymmetric reactions discussed,
while also enriching alkaloids chemistry, providing new ideas for the design and synthesis of
chiral catalysts, and providing abundant experimental data for the chiral induction process.
Influence of Inosine on Cerebral Hemodynamics in Space Motion Sickness in Experimental Animals
Authors: Useinova Asie N. , Kaliberdenko Vitalii B. , Sapegin Igor D. , Kulanthaivel Shanmugaraj , Shterenshis Michael V. , Dmitriyevskaya Maria I. , Balasundaram Keerthanaa 
Published: April 2021

Background:
Motion sickness occurs worldwide in healthy individuals regardless of age,
ethnicity, or gender. It is an acute disorder; it can also present as a chronic disorder in some individuals.
Motion sickness not only includes vomiting and nausea, but also includes other features such as
pallor of varying degrees, cold sweating, headache, drowsiness, increased salivation, and cranial pain,
which are severe. Some of the other assessment scales can interpret sickness on exposure to virtual or
visual stimulation and while travelling in different types of transport.

Aim:
The aim of our research is to study the effect of the drug on the level of blood flow and vascular
reactivity of cerebral vessels when simulating changes in the cerebral circulation in terrestrial conditions
characteristic of hypogravity.

Methods:
Chronic experiments were performed on non-anesthetized rabbits with large hemispheres,
thalamus and hypothalamus were implanted with the needle-platinum electrodes 150 mm in diameter
in the cortex, and local blood flow and vascular reactivity were recorded accordingly. Cerebrovascular
disturbances were modeled using an MSAOP (motion sickness of animals in the anti-orthostatic position)
with an inclined angle of 45° for 2 hours. Local blood flow (BF) was measured in ml/min/100g
of tissue by the method of registration of hydrogen clearance. The vasodilator coefficient of reactivity
(CrCO2) was calculated by the ratio of BF against the background of inhalation of a mixture of 7%
CO2 with air to the initial BF; vasoconstrictor - in relation to BF on the background of inhalation of
100% O2 to the initial BF (CrO2). A series of experiments were carried out with different routes of
drug administration: First, inosine was administered intravenously at a dose of 5 mg/kg immediately
before the start of SMS modeling, same dose was administered 30 minutes before the start of exposure.
As a control, we used the results of experimental animals under similar conditions without the
administration of the drugs.

Results:
Inosine has pronounced protective properties in cerebrovascular disorders on the background
of space motion sickness (SMS) modeling, which is manifested by normalization of BF and restoration of
compensatory reactions of cerebral vessels. In the mechanism of cerebroprotective action of inosine, it is
able to correct the metabolic processes, which play an important role and help increase the compensatory
capabilities and functional stability of the cerebrovascular system under gravitational influences.

Conclusion:
When using inosine orally, the effects are more pronounced than when administered intravenously,
which should be taken into account when using it for the prevention of cerebrovascular
disorders in extreme conditions.
Nanotechnology and Acoustics in Medicine and Biology
Authors: Abedini-Nassab Roozbeh , Emami Seyed Mohammadhossein , Nowghabi Arshia Naeimi 
Published: April 2021

Background:
Nanotechnology plays an important role in various engineering fields,
one of which is acoustics.

Method:
Here, we review the use of nanotechnology in multiple acoustic-based bioapplications,
with a focus on recent patents and advances. Nanoparticles, nanorods, nanotubes, and nanofilms
used in acoustic devices are discussed. We cover ultrasonic transducers, biosensors, imaging
tools, nanomotors, and particle sorters.

Results and conclusion:
The way these ideas help in fundamental disciplines such as medicine is
shown. We believe the current work is a good collection of advances in the field.