bluuebunny/crossref_metadata_2025_split huggingface dataset:
Preparation of immobilized arylsulfatase on magnetic Fe3O4 nanoparticles and its application for agar quality improvement
Authors: Zhang Chenghao , Jiang Zedong , Li Hebin , Ni Hui , Zheng Mingjing , Li Qingbiao , Zhu Yanbing 
Published: July 2021

AbstractThe presence of sulfate groups in agar compromises the agar quality by affecting the crosslinking during gelling process. Some arylsulfatases can catalyze the hydrolysis of sulfate bonds in agar to improve the agar quality. Immobilized arylsulfatases prove beneficial advantages for their industrial applications. Here, a previously characterized mutant arylsulfatase K253H/H260L was immobilized on the synthesized magnetic Fe3O4 nanoparticles after functionalization by tannic acid (MNPs@TA). The surface properties and molecular structures of the immobilized arylsulfatase (MNPs@TA@ARS) were examined by scanning electron microscopy and Fourier transform infrared spectroscopy. Enzymatic characterization showed that MNPs@TA@ARS exhibited shifted optimal temperature and pH with deviated apparent Km and Vmax compared to its free counterpart. The immobilized arylsulfatase demonstrated improved thermal and pH stability and enhanced storage stability with modest reusability. In addition, MNPs@TA@ARS displayed enhanced tolerance to various inhibitors and detergents. The utilization of the immobilized arylsulfatase for agar desulfation brought the treated agar with improved quality.
Prediction of top round beef meat tenderness as a function of marinating time based on commonly evaluated parameters and regression equations
Authors: Rostamani Mohammadreza , Baghaei Homa , Bolandi Marzieh 
Published: July 2021

AbstractThis study investigates the influence of 24‐hr marination (with different plant extracts and vinegar) at refrigerated conditions on commonly evaluated physicochemical and textural attributes, including pH, water‐holding capacity (WHC), collagen solubility, moisture, drip loss, and shear force values of beef meat. The results reflected the appropriate correlation between each pair and indicated the efficiency of the household marination procedure to acquire more palatability and tender beef meat. Therefore, to predict beef meat tenderness by applying the Warner–Bratzler shear force (WBSF), a strong positive correlation with the drip loss (p < .01) and a notable negative correlation with the moisture content (p < .01) emphasized the importance of moisture improvement and shear force reduction in affecting tenderness of baked beef meat. The regression equations and R‐squared values were revealed the favorable correlation between collagen solubility and WHC (y = 0.1035x−0.8431, R2 = .98) as well as moisture and WBSF (y = −0.3297x + 102.58, R2 = .99) in marinated beef meat. Electrophoresis patterns of isolated myofibrillar proteins disclosed remarkable degradation of myosin heavy chain (MHC), desmin, actin, and tropomyosin during the first day of aging. The noticeable ultrastructural destruction and connective tissue solubilization were observed by microscopy images. These outcomes were a good tenderness predictor be utilized in retailing and industrial scale.
The impact of environmental and occupational exposures of manganese on pulmonary, hepatic, and renal functions
Authors: Gandhi Deepa , Rudrashetti Ashwinkumar P. , Rajasekaran Subbiah 
Published: July 2021

AbstractManganese (Mn) is an essential trace element for humans, but long‐term environmental or occupational exposures can lead to numerous health problems. Although many studies have identified an association between Mn exposures and neurological abnormalities, emerging data suggest that occupationally and environmentally relevant levels of Mn may also be linked to multiple organ dysfunction in the general population. In this regard, many experimental and clinical studies provide support for a causal link between Mn exposure and structural and functional changes that are responsible for organ dysfunction in major organs like lung, liver, and kidney. The underlying mechanisms suggested to Mn toxicity include altered activities of the components of intracellular signaling cascades, oxidative stress, apoptosis, affected cell cycle regulation, autophagy, angiogenesis, and an inflammatory response. We further discussed the sources and possible mechanisms of Mn absorption and distribution in different organs. Finally, treatment strategies available for treating Mn toxicity as well as directions for future studies were discussed.
The cell membrane as the barrier in the defense against nanoxenobiotics: Zinc oxide nanoparticles interactions with native and model membrane of melanoma cells
Authors: Czyżowska Agnieszka , Barbasz Anna , Rudolphi‐Szydło Elżbieta , Dyba Barbara 
Published: July 2021

AbstractCurrently, we are dealing with ever‐increasing pollution of the environment with metal and metal oxide nanoparticles. One type of these, zinc oxide nanoparticles (ZnO‐NPs), are increasingly used in areas such as cosmetology, electrical engineering, medicine, and even in the food and textile industries. As a consequence, ZnO‐NPs may enter the human body in many ways. Their influence on the body is still not clear. Here, we define the mechanism of the initial toxicity of ZnO‐NPs to cells based on interaction with the lipid part of the native and model cell membrane. The selected cell lines react differently to contact with nanoparticles. We found a disruption of the native membranes of B16‐F0 cells and to a lesser extent of COLO 679. In turn, the membrane of COLO 679 cells was more peroxidated, and cell viability was much lower. A model of the lipid part of the membrane was created for B16‐F0 cells and compared with previously published studies on immune cells. On the basis of physicochemical parameters obtained for individual lipids and a mix representing the native membrane of the tested cells, we concluded that exposure to nanoparticles resulted in a change within the model membranes (specifically with the polar parts of lipids). The greatest interaction has been noticed between ZnO‐NPs and zwitterionic phospholipids (PC and PE), cholesterol, and negatively charged phosphatidylglycerol. Assessing the interactions between the membrane and nanoparticles will help to better understand the first steps of its toxicity mechanism.
A mixed‐methods approach to understanding trajectories of mentoring relationship growth
Authors: Spiekermann Lara , Lyons Michael , Deutsch Nancy 
Published: July 2021

AbstractThe proposed study integrates quantitative and qualitative approaches to examine mentors with different relationship trajectories reflect on their relationships. Using quantitative and qualitative methods, mentor reports of relationship quality are plotted over time and different growth patterns identified: (1) progressive, (2) stable‐high, (3) dip and recovery, (4) stable‐low, and (5) regressive. Qualitative coding was used to identify patterns in mentors' descriptions of their relationship experiences—including both what mentors wrote about and how they wrote about it.
Regulation of autophagy by protein methylation and acetylation in cancer
Authors: Jeon Minsol , Park Jisu , Yang Eunbi , Baek Hee‐ji , Kim Hyunkyung 
Published: July 2021

AbstractAutophagy is a highly conserved mechanism responsible for cellular homeostasis and integrity in a variety of physiological conditions. Materials targeted for degradation are directed to autophagosomes and autolysosomes, where they are broken down into their base components. Aberrant regulation of autophagy is significantly associated with various cancers and neurodegenerative diseases. Recently, accumulating evidence has revealed that the coordinated regulation of histone and non‐histone protein modification is associated with autophagy. In this review, we highlight the recent progress that has been made in elucidating the molecular basis of protein methylation and acetylation associated with autophagy at the transcriptional and posttranslational levels. Furthermore, we discuss the importance of describing causality between protein methylation/acetylation and autophagy regulation as compelling therapeutic opportunities in cancer pathogenesis and progression.
Arylquinolinecarboxamides: Synthesis, in vitro and in silico studies against Mycobacterium tuberculosis
Authors: Bokosi Fostino R. B. , Beteck Richard M. , Jordaan Audrey , Seldon Ronnet , Warner Digby F. , Tshiwawa Tendamudzimu , Lobb Kevin , Khanye Setshaba D. 
Published: July 2021

AbstractA series of fourteen 6‐substituted‐2‐(methoxyquinolin‐3‐yl) methyl)‐N‐(pyridin‐3‐ylmethyl) benzamides was prepared from commercially available anilines in five simple and convenient synthetic steps. The structures of all new products were confirmed by routine spectroscopic methods: IR, 1H and 13C NMR, and HRMS (electrospray ionization). The resulting arylquinolinecarboxamides were subjected to biological screening assay for in vitro inhibitory activity against Mycobacterium tuberculosis (Mtb) H37Rv strain. Several compounds exhibited modest antitubercular activity with compounds 8–11, 15 and 19 exhibiting MIC90 values in the range of 32–85 μM. The antitubercular data suggested that inhibition of Mtb can be imparted by the introduction of a non‐polar substituent on C‐6 of the quinoline scaffold. Further, to understand the possible mode of action of the series, the reported compounds and bedaquiline were subjected to in silico docking studies against MtbATPase to determine their potential to interfere with the mycobacterial adenosine triphosphate (ATP) synthase. The results showed that these compounds have the potential to serve as antimycobacterial agents. In silico ADME pharmacokinetic prediction results showed the ability of these arylquinolinecarcboxamides to be absorbed, distributed, metabolized and excreted efficiently.
Perceived Racism and Discrimination Among People of Color: An Ethnographic Content Analysis
Authors: Evans Amanda M. , Hemmings Carrie , Ramsay‐Seaner Kristine , Barnett Juanita 
Published: July 2021

As the counseling profession calls for multicultural competence, it is important to examine racial discrimination. We used ethnographic content analysis to examine the lived experiences of 28 people of color regarding racial discrimination. Themes derived from this study included stereotypes and racial profiling, exclusion or denigration, denial of institutional power and privilege, insults regarding physical appearance and language, and internalized racism. Recommendations for researchers and counselors are discussed.Dada la demanda de competencia multicultural en el ejercicio profesional de la consejería, es importante examinar la discriminación racial. Usamos un análisis de contenido etnográfico para examinar las experiencias vitales de 28 personas de color en cuanto a discriminación racial. Los temas derivados de este estudio incluyen estereotipos y caracterización racial, exclusión o denigración, negación de poder institucional y privilegios, insultos relacionados con la apariencia física y el idioma, así como el racismo interiorizado. Se discuten las recomendaciones para investigadores y consejeros.
The Effect of Multicultural Acceptance on Social Problem Solving Among University Students
Authors: Lee Oan Na , Song Chiang‐Soon 
Published: July 2021

This study examined the effect of multicultural acceptance on social problem solving in a sample of 159 university students in South Korea. A simple regression analysis revealed that multicultural acceptance significantly predicted social problem solving. Specifically, multicultural acceptance positively predicted positive problem orientation and rational problem solving. On the other hand, multicultural acceptance negatively predicted negative problem orientation, impulsivity‐carelessness style, and avoidance style. The limitations and implications of the results are discussed.Este estudio examinó el efecto de la aceptación multicultural sobre la resolución de problemas sociales en una muestra de 159 estudiantes universitarios en Corea del Sur. Un análisis de regresión simple reveló que la aceptación multicultural predijo significativamente la resolución de problemas sociales. Concretamente, la aceptación multicultural predijo de forma positiva una orientación positiva hacia los problemas y una resolución racional de los mismos. Por otra parte, la aceptación multicultural predijo de forma negativa una orientación negativa hacia los problemas, un estilo impulsivo‐descuidado y un estilo de evasión. Se discuten las limitaciones e implicaciones de los resultados.
Diagnostic accuracy of prospectively gated, 128‐slice or greater CTCA at high heart rates: a systematic review and meta‐analysis
Authors: Mander Gordon T.W. , Dobeli Karen , Steffensen Caitlin , Munn Zachary 
Published: July 2021

AbstractIntroductionProspectively gated 64‐slice CT coronary angiography (CTCA) may be contraindicated for heart rates (HRs) over 65 beats per minute (bpm) due to reduced diagnostic sensitivity. Newer CT scanners typically provide 128 or more slices and superior temporal resolution compared with older models; consequently, diagnostic accuracy for current technology prospectively gated CTCA may be adequate at HRs above 65 bpm. The aim of this systematic review was to investigate the diagnostic accuracy of CTCA using 128‐slice or greater CT technology when compared with conventional coronary angiography for patients with HRs >65 bpm.MethodsA systematic search of PubMed, CINAHL, EMBASE and Scopus was performed as well as unpublished databases, sources and reference lists. Titles and abstracts were screened by two independent reviewers. Full‐text screening was then performed. Studies that determined diagnostic accuracy of coronary artery stenosis in adult patients with high heart rates utilising prospectively gated 128 detector or greater scanners were included. Studies that were included in the review underwent critical appraisal using the QUADAS‐2 tool.ResultsTen studies were included in the systematic review, with nine of these included in a diagnostic test accuracy meta‐analysis, including six of which reported data at the patient level. Meta‐analysis indicated very high pooled sensitivity 100% (95% CI 0.99, 1.00); however, pooled specificity was less at 79% (95% CI 0.69, 0.88).ConclusionsProspectively gated CT coronary angiography may be justifiable at heart rates above 65 bpm if performed on a 128‐slice or greater CT unit. Caution regarding the implication of a positive result is recommended due to reduced specificity. Further evidence is required before consideration of a new higher heart threshold.
Novel Multidisciplinary Salivary Gland Society (MSGS) Questionnaire: An International Consensus
Authors: Buchholzer Samanta , Faure Frédéric , Tcheremissinoff Livia , Herrmann François R. , Lombardi Tommaso , Ng Siu‐Kwan , Lopez Jean‐Michel , Borner Urs , Witt Robert L. , Irvine Robert , Abboud Olivier , Cernea Claudio R. , Ghan Shirish , Matsunobu Takeshi , Ahmad Zahoor , Morton Randall , Anicin Aleksandar , Magdy Emad A. , Al Abri Rashid , Konstantinidis Iordanis , Capaccio Pasquale , Klein Hila , Poorten Vincent Vander , Lombardi Davide , Lyons Bernard , Al Rand Hussain , Liao George , Kim Jeong K. , Subha Sethu , Su Richard Y.‐X. , Su Chin‐Hui , Boselie Franciscus , Andre Raphaël , Seebach Jörg D. , Marchal Francis 
Published: July 2021

ObjectivesFirst, establishment and validation of a novel questionnaire documenting the burden of xerostomia and sialadenitis symptoms, including quality of life. Second, to compare two versions regarding the answering scale (proposed developed answers Q3 vs. 0–10 visual analogue scale Q10) of our newly developed questionnaire, in order to evaluate their comprehension by patients and their reproducibility in time.Study DesignThe study is a systematic review regarding the evaluation of the existing questionnaire and a cohort study regarding the validation of our new MSGS questionnaire.Materials and MethodsA Multidisciplinary Salivary Gland Society (MSGS) questionnaire consisting of 20 questions and two scoring systems was developed to quantify symptoms of dry mouth and sialadenitis. Validation of the questionnaire was carried out on 199 patients with salivary pathologies (digestive, nasal, or age‐related xerostomia, post radiation therapy, post radioiodine therapy, Sjögren's syndrome, IgG4 disease, recurrent juvenile parotitis, stones, and strictures) and a control group of 66 healthy volunteers. The coherence of the questionnaire's items, its reliability to distinguish patients from healthy volunteers, its comparison with unstimulated sialometry, and the time to fill both versions were assessed.ResultsThe novel MSGS questionnaire showed good internal coherence of the items, indicating its pertinence: the scale reliability coefficients amounted to a Cronbach's alpha of 0.92 for Q10 and 0.90 for Q3. The time to complete Q3 and Q10 amounted, respectively, to 5.23 min (±2.3 min) and 5.65 min (±2.64 min) for patients and to 3.94 min (±3.94 min) and 3.75 min (±2.11 min) for healthy volunteers. The difference between Q3 and Q10 was not significant.ConclusionWe present a novel self‐administered questionnaire quantifying xerostomia and non‐tumoral salivary gland pathologies. We recommend the use of the Q10 version, as its scale type is well known in the literature and it translation for international use will be more accurate. Laryngoscope, 132:322–331, 2022
ATH434Reduces α‐Synuclein‐Related Neurodegeneration in a Murine Model of Multiple System Atrophy
Authors: Heras‐Garvin Antonio , Refolo Violetta , Schmidt Claudio , Malfertheiner Katja , Wenning Gregor K. , Bradbury Margaret , Stamler David , Stefanova Nadia 
Published: July 2021

ABSTRACTBackgroundMultiple system atrophy (MSA) is a fatal neurodegenerative disorder characterized by aggregated α‐synuclein (α‐syn) in oligodendrocytes and accompanied by striatonigral and olivopontocerebellar degeneration and motor symptoms. Key features of MSA are replicated in the PLP‐α‐syn transgenic mouse, including progressive striatonigral degeneration and motor deterioration. There are currently no approved treatments for MSA. ATH434 is a novel, orally bioavailable brain penetrant small molecule inhibitor of α‐syn aggregation.ObjectivesTo characterize ATH434 for disease modification in a mouse model of MSA.MethodsSix‐month‐old PLP‐α‐syn mice (MSA mice) were ATH434‐treated (ATH434 in food) or untreated (normal food) for 6 months. Motor behavior and numbers of nigral and striatal neurons were evaluated. α‐syn aggregates and oligomers were quantified by immunohistochemical and western blot analyses. Microglial activation and neuroinflammation were assessed by histological and molecular analyses. Ferric iron in the Substantia nigra was evaluated with the Perls method.ResultsATH434‐treated mice demonstrated preservation of motor performance in MSA mice that was associated with neuroprotection of nigral and striatal neurons. The rescue of the phenotype correlated with the reduction of α‐syn inclusions and oligomers in animals receiving ATH434. ATH434‐treated mice exhibited significantly increased lysosomal activity of microglia without increased pro‐inflammatory markers, suggesting a role in α‐syn clearing. ATH434‐treatment was associated with lower intracellular nigral iron levels.ConclusionsOur findings demonstrate the beneficial disease‐modifying effect of ATH434 in oligodendroglial α‐synucleinopathy on both the motor phenotype and neurodegenerative pathology in the PLP‐α‐syn transgenic mouse and support the development of ATH434 for MSA. © 2021 The Authors.Movement Disorderspublished by Wiley Periodicals LLC on behalf of International Parkinson and Movement Disorder Society
How reliable are TIGGE daily deterministic precipitation forecasts over different climate and topographic conditions of Iran?
Authors: Amini Setareh , Azizian Asghar , Daneshkar Arasteh Peyman 
Published: July 2021

AbstractAccurate forecasting of precipitation has been a very crucial issue for developing meteorological and hydrological early warning systems. Ensemble forecasts using numerical weather prediction models are promising for these purposes. This research addressed the reliability of global precipitation forecasts of the THORPEX (The Observing System Research and Predictability Experiment) Interactive Grand Global Ensemble (TIGGE) archive over distinct climate and topographic regions of Iran. Besides, post‐processing the raw forecasts and assessing TIGGE datasets' performance in predicting extreme rainfall events, at the thresholds of 10–20, 20–50, 50–100, and higher than 100 mm, are other objectives of this study. Results indicated that the European Centre for Medium‐Range Weather Forecasts (ECMWF) and Meteo France forecasts, with the mean correlation coefficients (CCs) of 0.64 and 0.61, were closer to the ground‐gauge observations, especially in different types of humid zones. On the contrary, in semi‐arid and extra‐arid climate zones, the CC value was relatively low. Furthermore, Meteo France, Korea Meteorological Administration, Japan Meteorological Agency and ECMWF outperformed other models in detecting rainy or non‐rainy days. Also, by increasing the elevation, the correlation between forecasts and in situ observations increased significantly, and the percentage increment of CC for ECMWF and Meteo France models is higher than 38%. Moreover, the skill of predictions for extreme events (higher than 50 mm) decreased meaningfully, while at the thresholds of 10–20 and 20–50 mm, ECMWF and China Meteorological Administration outperformed other models. Finally, post‐processing of raw forecasts using quantile mapping improved the CC and relative bias metrics up to 30% and 51%, respectively, especially in extra‐arid, semi‐arid and Mediterranean climate zones.
Manipulating pattern periods via external bias for graphene‐based THz Dual‐Band absorber
Authors: Aghaee Toktam , Orouji Ali A. 
Published: July 2021

AbstractElectrical control of terahertz (THz) radiation for high‐performance THz imaging is the key point. The spectrum suffers from a lack of active and available materials such as graphene have a severely limited bias range leading to restricted tuning. Therefore, here we demonstrated an efficient tunable device exploiting a triple bias layer for patterned graphene ribbons. Here, the patterned graphene acts as a tunable surface impedance leads to electrically controlled impedance matching between the device and free space. So, a dual‐band absorption is obtained which is appropriately robust against incident angle and capable of adjusting absorption peaks. The major contribution of the design is capability of manipulating patterns periods via gate biasing. This causes providing additional degree of freedom compared to conventional graphene patterns. According to the simulation results, the proposed device shows excellent tuning operation via gate bias voltages.
A Fibonacci series‐inspired‐elliptically‐slotted CPW fed UWB patch antenna with Ku band‐reject characteristics
Authors: Behera Ananda Kumar , Konhar Diptimayee , Mishra Debasis , Mishra Suvendu Narayan 
Published: July 2021

AbstractThis letter introduces a compact elliptically‐slotted patch antenna with Ku band reject characterizes. The proposed antenna works in the super wide range of frequency band stretched beyond the UWB. The slots in the radiating patch take the shape of ellipse and are present in such a manner that the double of their ellipticity follow the Fibonacci series. The perturbed‐diamond‐shaped patch without the slot radiates in the frequency range from 4.19 to 21.88 GHz with a bandwidth of 17.67 GHz. The same patch antenna with the elliptical‐slots rejects Ku band from 12.89 to 16.22 GHz. We have analyzed the notch frequency variation with respect to the slot area using Ansys HFSS V.20 electromagnetic simulator software for verification.