bluuebunny/crossref_metadata_2025_split huggingface dataset:
Aligning integrated ecosystem assessment with adaptation planning in support of ecosystem-based management
Authors: Woods Pamela J 
Published: June 2021

Abstract
Supporting resilience is a common goal of natural resource management, but managing under changing conditions that requires adaptation is a modern challenge. A state-of-the-art framework for implementing an integrated ecosystem assessment (IEA), the NOAA IEA approach, is used as an example to demonstrate whether and how assessment in ecosystem-based management (EBM), as often implemented in fisheries, can be expected to facilitate planned adaptation. Using comparisons with another assessment framework developed for implementing a climate change adaptation project, the United Nations Development Programme-Global Environment Facility Adaptation Policy Framework (UNDP-GEF APF), this paper expands and operationalizes the concepts of managing for resilience versus change in EBM as presented by West et al.(2009). It first introduces a variety of terms from climate change adaptation literature to help institutionalize “planned adaptation” as a useful concept within fisheries, then presents an expanded map of adaptive management processes in EBM. Finally, it proposes steps for enhancing processes supporting planned adaptation in individual applications of EBM in fisheries. Steps include (i) recognizing interest and funding for adaptation planning as prerequisites, (ii) evaluating what information or actors are lacking to implement better planning, and (iii) determining what institutional processes within an adaptive management cycle need augmentation.
Performance of disc diffusion, MIC gradient tests and Vitek 2 for ceftolozane/tazobactam and ceftazidime/avibactam susceptibility testing ofPseudomonas aeruginosa
Authors: Daragon Brice , Fournier Damien , Plésiat Patrick , Jeannot Katy 
Published: June 2021

AbstractObjectivesTo assess performance of disc diffusion, gradient tests and Vitek 2 system to determine the susceptibility of clinical Pseudomonas aeruginosa strains to ceftolozane/tazobactam (C/T) and ceftazidime/avibactam (CZA).MethodsTwo-hundred non-duplicate P. aeruginosa strains isolated by 47 French medical laboratories were selected to cover a wide range of C/T and CZA MICs. Performance of C/T disc (30/10 μg, Bio-Rad), CZA discs (10/4 μg) (Thermo Fisher and Bio-Rad), C/T and CZA gradient tests (Etest, BioMérieux; MIC Test Strip, Liofilchem), and AST-XN12 card of Vitek 2 system (BioMérieux) were compared with a broth microdilution (BMD) method (Thermo Fisher). MIC and disc results were interpreted using current EUCAST breakpoints.ResultsTwenty percent and 17% of strains were resistant to C/T and CZA, respectively. All the methods tested satisfactorily determined the susceptibility of P. aeruginosa to C/T [Category Agreement (CA) ≥95%] except the disc diffusion method. Because of the high rates of Major Errors (MEs) (12.5%), this latter method tends to overestimate the resistance. For CZA, only the gradient tests yielded more than 90% of CA. The Vitek 2 system and disc diffusion misclassified 18.1%, 10.1% (disc Bio-Rad) and 11.9% (disc Thermo Fisher) of susceptible strains, respectively.ConclusionsThe gradient tests (MIC Test Strip and Etest) and Vitek 2 card XN12 performed the best to determine the susceptibility of P. aeruginosa to C/T, whereas gradient tests were an acceptable alternative to BMD to assess CZA susceptibility.
Patent Assertion Entities and Patent Ownership Transparency: Strategic Recording of Patent Transactions at the Uspto
Authors: Sterzi Valerio 
Published: June 2021

AbstractMany patent assertion entities (PAEs) hide behind multiple unknown subsidiaries or shell companies with obscure ownership. Meanwhile, the United States Patent and Trademark Office (USPTO), like many other patent offices, does not impose a strict time period for recording the change of ownership of a patent, allowing the holder to gain an advantage by controlling the timing of its ownership disclosure. In this paper, we study to which extent PAEs delay the recording of the U.S. patent reassignments of patents that they will subsequently use in infringement patent lawsuits. On average, PAEs notify the change of ownership more quickly than producing firms, but this is not the case for the patents that they will litigate relatively far in time. In particular, the correlation between the recording lag of the patent transaction and the litigation spell is higher when the patent acquirer is a PAE (than when it is a producing firm) or when the acquirer will litigate the patent in the Eastern District of Texas, famously home to opportunistic litigations. Finally, we find that transactions involving unknown subsidiaries of PAEs are recorded at the USPTO significantly later than those involving PAE parent companies or their known subsidiaries.
Determining the impact of vitamin C use with the common cold on loss of labour and medical treatment costs for Turkey
Authors: Oğuzhan Gülpembe , Ökçün Selin , Kurnaz Mustafa , Koçkaya Güvenç , Şen Selçuk , Kahveci Kaplan Burçin , Mete Şaylan İsmail 
Published: June 2021

Abstract

Objectives
As one of the most common health problems, the common cold may lead to negative consequences such as work or school absenteeism, poor productivity and labour loss. Vitamin C supports immune system defences by supporting the various cellular functions of the immune system, both congenital and adaptive. The purpose of this study is to evaluate the use of vitamin C in white-collar workers and its impact on medical care costs and its impact on the workforce in association with the common cold.

Methods
For calculation of the effect of 1000 mg vitamin C daily on the workforce and possible healthcare services costs, a Markov-based model was simulated with 6 cycles consistent with the literature that constituted the model input for the 6-month common cold season. The simulated Markov model included parameters for the number of seasonal flu episodes with or without vitamin C use, the duration of the episodes, productivity loss and daily labour costs. The TreeAge Healthcare Pro 2020 program was used for the Markov model.

Key findings
The calculations revealed that the common cold lasted 0.47 days less with vitamin C use. The calculations revealed that the number of days absent from work is 0.38 days more without vitamin C use compared with vitamin C use. The calculations revealed that the medical treatment costs for the common cold is TL 1723.98. According to the analysis conducted based on the Markov model, the total cost of lost productivity and medical treatment that would occur in case of a common cold is TL 3704.97 with vitamin C use and TL 4223.26 without vitamin C use.

Conclusion
Based on the results of the analysis, we can suggest that regular vitamin C supplementation may prevent the impact of the common cold on public health and the resulting economic burden.
Capitalism as Death: Loss of Life and the Finnish Migrant Left in the Early Twentieth Century
Authors: Saramo Samira 
Published: June 2021

Abstract
In early twentieth-century Canada and the United States, Finnish migrants faced dangerous working conditions and regularly lost lives on the job. To counter government and company inaction, migrant workers supported each other through grassroots community systems of reciprocity and participation in unionism and socialism. This article pairs migrant labor history with the history of death and mourning to explore how the relationship between the two may mutually develop our understandings of everyday life. Three case studies are at the center of analysis: the Italian Hall Tragedy of 1913 (Calumet, Michigan), the Hollinger Gold Mine Disaster of 1928 (Timmins, Ontario), and the deaths of lumber union organizers Viljo Rosvall and Janne Voutilainen in 1929 (Thunder Bay District, Ontario). By focusing on the death, grief, and mourning at the core of these events and on the days immediately following the tragedies, I demonstrate that death and loss were central to Finnish migrant workers’ everyday encounters with community and class-consciousness. I analyze newspaper coverage of these deaths in order to investigate the strategies of the Finnish language socialist press and leadership to emphasize the deadly power of the capitalist socioeconomic structure, but also seek the everyday spaces, feelings, and relationships caught among labor tensions. In analyzing the cases, I aim to highlight opportunities for new types of dialogue on migrant social history that come from bringing together death, the everyday, and the political.
Gibberellin biosynthesis inhibitor mepiquat chloride enhances root K+ uptake in cotton by modulating plasma membrane H+-ATPase
Authors: Zhang Rui , Wang Ning , Li Shuying , Wang Yiru , Xiao Shuang , Zhang Yichi , Egrinya Eneji A , Zhang Mingcai , Wang Baomin , Duan Liusheng , Li Fangjun , Tian Xiaoli , Li Zhaohu 
Published: June 2021

AbstractPotassium deficiency causes severe losses in yield and quality in crops. Mepiquat chloride, a plant growth regulator, can increase K+ uptake in cotton (Gossypium hirsutum), but the underlying physiological mechanisms remain unclear. In this study, we used a non-invasive micro-test technique to measure K+ and H+ fluxes in the root apex with or without inhibitors of K+ channels, K+ transporters, non-selective cation channels, and plasma membrane H+-ATPases. We found that soaking seeds in mepiquat chloride solution increased the K+ influx mediated by K+ channels and reduced the K+ efflux mediated by non-selective cation channels in cotton seedlings. Mepiquat chloride also increased negative membrane potential (Em) and the activity of plasma membrane H+-ATPases in roots, due to higher levels of gene expression and protein accumulation of plasma membrane H+-ATPases as well as phosphorylation of H+-ATPase 11 (GhAHA11). Thus, plasma membrane hyperpolarization mediated by H+-ATPases was able to stimulate the activity of K+ channels in roots treated with mepiquat chloride. In addition, reduced K+ efflux under mepiquat chloride treatment was associated with reduced accumulation of H2O2 in roots. Our results provide important insights into the mechanisms of mepiquat chloride-induced K+ uptake in cotton and hence have the potential to help in improving K nutrition for enhancing cotton production.
Far-UV Fe emission as proxy of Eddington ratios
Authors: Zheng Wei 
Published: June 2021

ABSTRACT
The Eddington ratio is a key parameter that governs the diversity of quasar properties. It can be scaled with a strong anticorrelation between optical Fe iiopt and [O iii] emission. In search of such indicators in the far-UV band, the Hubble Space Telescope far-UV spectra of 150 low-redshift quasars are analysed in combination with their optical SDSS counterparts. The strength of Fe ii+Fe iii λ1123 emission is significantly correlated with that of Fe iiopt. A moderate correlation may also exist between Fe ii λ1071 and Fe iiopt. The finding opens the possibility that far-UV Fe ii emission may serve as a new gauge of the Eddington ratios. The high- and low-ionization lines in the far-UV band display different patterns: for the quasars with higher Eddington ratios, the low-ionization UV lines are stronger, and the high-ionization lines are broader and weaker.
Single-cell nucleic acid profiling in droplets (SNAPD) enables high-throughput analysis of heterogeneous cell populations
Authors: Hyman Leland B , Christopher Clare R , Romero Philip A 
Published: June 2021

Abstract
Experimental methods that capture the individual properties of single cells are revealing the key role of cell-to-cell variability in countless biological processes. These single-cell methods are becoming increasingly important across the life sciences in fields such as immunology, regenerative medicine and cancer biology. In addition to high-dimensional transcriptomic techniques such as single-cell RNA sequencing, there is a need for fast, simple and high-throughput assays to enumerate cell samples based on RNA biomarkers. In this work, we present single-cell nucleic acid profiling in droplets (SNAPD) to analyze sets of transcriptional markers in tens of thousands of single mammalian cells. Individual cells are encapsulated in aqueous droplets on a microfluidic chip and the RNA markers in each cell are amplified. Molecular logic circuits then integrate these amplicons to categorize cells based on the transcriptional markers and produce a detectable fluorescence output. SNAPD is capable of analyzing over 100,000 cells per hour and can be used to quantify distinct cell types within heterogeneous populations, detect rare cells at frequencies down to 0.1% and enrich specific cell types using microfluidic sorting. SNAPD provides a simple, rapid, low cost and scalable approach to study complex phenotypes in heterogeneous cell populations.
Unifying Phylogenetic Birth–Death Models in Epidemiology and Macroevolution
Authors: MacPherson Ailene , Louca Stilianos , McLaughlin Angela , Joy Jeffrey B , Pennell Matthew W 
Published: June 2021

Abstract
Birth–death stochastic processes are the foundations of many phylogenetic models and are widely used to make inferences about epidemiological and macroevolutionary dynamics. There are a large number of birth–death model variants that have been developed; these impose different assumptions about the temporal dynamics of the parameters and about the sampling process. As each of these variants was individually derived, it has been difficult to understand the relationships between them as well as their precise biological and mathematical assumptions. Without a common mathematical foundation, deriving new models is nontrivial. Here, we unify these models into a single framework, prove that many previously developed epidemiological and macroevolutionary models are all special cases of a more general model, and illustrate the connections between these variants. This unification includes both models where the process is the same for all lineages and those in which it varies across types. We also outline a straightforward procedure for deriving likelihood functions for arbitrarily complex birth–death(-sampling) models that will hopefully allow researchers to explore a wider array of scenarios than was previously possible. By rederiving existing single-type birth–death sampling models, we clarify and synthesize the range of explicit and implicit assumptions made by these models. [Birth–death processes; epidemiology; macroevolution; phylogenetics; statistical inference.]
Testing the feasibility and acceptability of a culturally adapted physical activity intervention for adult Somali women
Authors: Murray Kate E , Hellier Villafana Veronica Anne , Sheik Mohamed Amina , Linke Sarah , Bowen Deborah J , Marcus Bess 
Published: June 2021

Abstract
Despite growing numbers in the USA, immigrant populations are underrepresented in existing physical activity (PA) research, in particular Muslim immigrant women. The current study is a pilot evaluation of a culturally adapted evidence-based PA intervention for adult Somali women. Stratified randomization was used to assign participants from a sample of 27 Somali women, aged 18 to 65, to a PA group or a waitlist control group. Bicultural Somali community research team members delivered a 12-week culturally adapted intervention available in English and Somali in a community-based setting. Process and outcome evaluation assessed changes in PA, self-efficacy for PA, access to PA resources, and wellbeing as well as feasibility and satisfaction with the program. Participants in the PA group increased their moderate to vigorous PA significantly more than those from the waitlist group from baseline to post-intervention (2 (SD = 15) to 100 (SD = 53) vs 12 (SD = 21) to 32 (SD = 44) minutes per week). Participants in the PA group had significantly greater scores in wellbeing at post-intervention compared to the waitlist group though there was no significant change from pre- to post-intervention for either group. Participants reported a high level of satisfaction with the program and preliminary evidence supports the general feasibility and acceptability of the program. Findings show that a culturally adapted intervention increased engagement in PA and was feasible and acceptable within a pilot sample of Somali women.
Bisphenol S and Bisphenol F Are Less Disruptive to Cardiac Electrophysiology, as Compared With Bisphenol A
Authors: Prudencio Tomas M , Swift Luther M , Guerrelli Devon , Cooper Blake , Reilly Marissa , Ciccarelli Nina , Sheng Jiansong , Jaimes Rafael , Posnack Nikki Gillum 
Published: June 2021

Abstract
Bisphenol A (BPA) is a high-production volume chemical used to manufacture consumer and medical-grade plastic products. Due to its ubiquity, the general population can incur daily environmental exposure to BPA, whereas heightened exposure has been reported in intensive care patients and industrial workers. Due to health concerns, structural analogs are being explored as replacements for BPA. This study aimed to examine the direct effects of BPA on cardiac electrophysiology compared with recently developed alternatives, including BPS (bisphenol S) and BPF (bisphenol F). Whole-cell voltage-clamp recordings were performed on cell lines transfected to express the voltage-gated sodium channel (Nav1.5), L-type voltage-gated calcium channel (Cav1.2), or the rapidly activating delayed rectifier potassium channel (hERG). Cardiac electrophysiology parameters were measured using human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CM) and intact, whole rat heart preparations. BPA was the most potent inhibitor of fast/peak (INa-P) and late (INa-L) sodium channel (IC50 = 55.3, 23.6 µM, respectively), L-type calcium channel (IC50 = 30.8 µM), and hERG channel current (IC50 = 127 µM). Inhibitory effects on L-type calcium channels were supported by microelectrode array recordings, which revealed a shortening of the extracellular field potential (akin to QT interval). BPA and BPF exposures slowed atrioventricular (AV) conduction and increased AV node refractoriness in isolated rat heart preparations, in a dose-dependent manner (BPA: +9.2% 0.001 µM, +95.7% 100 µM; BPF: +20.7% 100 µM). BPS did not alter any of the cardiac electrophysiology parameters tested. Results of this study demonstrate that BPA and BPF exert an immediate inhibitory effect on cardiac ion channels, whereas BPS is markedly less potent. Additional studies are necessary to fully elucidate the safety profile of bisphenol analogs on the heart.
Exploring the Role of Calmodulin and Calcium Signaling in Leukocyte Transmigration
Authors: Dalal Prarthana J. , Sullivan David P. , Muller William A. 
Published: June 2021

Inflammation results in the dilation of local blood vessels, the activation of endothelium by cytokines and the recruitment of circulating leukocytes into areas of tissue damage. A transient increase in endothelial cytosolic free calcium (Ca2+) is required for leukocyte transendothelial migration (TEM) and transient receptor potential canonical 6 (TRPC6) has been found to mediate this required Ca2+ influx. Furthermore, we have also found that IQ‐domain GTPase‐activating protein 1 (IQGAP1) is essential for TEM. Specifically, the actin‐binding domain and the calmodulin‐binding isoleucine‐glutamine (IQ) domain of IQGAP1 are critical for its function during TEM. In endothelial cells re‐expression of IQGAP1 truncation mutants lacking the N‐terminal actin‐binding domain did not localize to endothelial borders and did not support TEM. Re‐expression of truncated IQGAP1 constructs containing the actin‐binding domain and the IQ domain localized to cell borders and supported TEM while constructs containing the actin‐binding domain but lacking the IQ domain localized to the cell borders but did not restore TEM.Calmodulin (CaM) is a small, highly conserved intracellular calcium‐binding protein, which has been shown to interact with IQGAP1 through the IQ domain. It has also been previously found to regulate TRPC channel activation; however, not much is known regarding its role in TEM. Therefore, we hypothesized that it may be involved in the calcium signaling cascade within endothelial cells essential for supporting leukocyte transmigration.Human endothelial cells treated with calmodulin inhibitor trifluoperazine (TFP) demonstrated a marked reduction in leukocyte TEM. Endothelial monolayers pretreated with 100 mM TFP for 1 hour drastically reduced leukocyte TEM. Only about 13% of leukocytes were able to transmigrate compared to 84% TEM in control, untreated monolayers (p=0.0025).After identifying the potential role of calmodulin, we generated CaM shRNA to deplete endogenous CaM within endothelial cells, wildtype Flag‐tagged CaM re‐expression constructs and CaM calcium‐binding mutants that we are currently in the process of testing. Identifying the specific mechanism by which endothelial Ca2+ influx through TRPC6 mediates the events of TEM will provide novel insight into the signaling involved in TEM and also provide potential therapeutic targets for anti‐inflammatory therapy.Support or Funding InformationF30HL134202, R01HL046849, R01HL064774This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.
CD99L2 as a Major Regulator in Human Transendothelial Migration
Authors: Rutledge Nakisha S. , Muller William A. 
Published: June 2021

Transendothelial Migration (TEM) is a crucial step in the inflammatory response as it is the process in which leukocytes leave the bloodstream to enter the inflamed tissues. Adhesion/signaling molecules such as PECAM and CD99 are important for the initiation and completion of TEM. We and others have shown that CD99‐like 2 (L2), a highly glycosylated 52 kD type‐1 membrane protein that is the only molecule in the genome related to CD99 is also important for TEM. However, L2 is different enough from CD99 (60% larger and with only 38% sequence identity) that it is not clear whether it regulates TEM by the same mechanism as CD99. Furthermore, all of the published studies on L2 have been performed in mice. The role of L2 in human cells is not known. In order to study the mechanism used by L2 and the relevance to human inflammation, we studied the role of L2 on human cells in vitro.Our data show that similar to PECAM and CD99, human L2 is constitutively expressed at the borders of endothelial cells and on the surface of leukocytes; treatment with proinflammatory cytokines changes neither its surface expression nor its distribution. Blocking L2 using antibodies significantly reduces transmigration of human neutrophils and monocytes across human endothelial cells. Blockade of either leukocytes or endothelial cells blocks equivalently to blocking both, consistent with a homophilic mechanism of interaction. Furthermore, knockdown of L2 using shRNAs dose‐dependently reduces TEM of neutrophils and monocytes across endothelial cells. TEM is restored by re‐expression of L2. Our data also show that L2 regulates a step in TEM after PECAM and before CD99. Consistent with this, preliminary data suggest that L2 can promote TEM by engaging either the signaling pathway used by PECAM or the one used by CD99. Similar to PECAM and CD99, L2 promotes transmigration by recruiting the lateral border recycling compartment to the site of TEM. Ongoing studies are focused on identifying unique signaling pathways (if any) used by L2 to promote TEM.Support or Funding InformationT32‐5T32AI007476‐19R37‐HL064774F31‐HL131355‐01This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.