bluuebunny/crossref_metadata_2025_split huggingface dataset:
Application of Iced Normal Saline Combined with Cocktail Perfusion in Total Knee Arthroplasty:Randomized Controlled Trialrandomized Controlled Trial
Authors: Sui Laijian , Jiang Aihua , Gai Pengzhou , Qi Xiaojun , Wang Jinwei , Wang Jing , Wang Xiufeng , Wang Guangda 
Published: August 2021

Abstract
Background: The present study was designed to investigate the safety and effectiveness of iced normal saline combined with cocktail perfusion during total knee arthroplasty (TKA).Method: Sixty patients undergoing TKA were divided into three groups, then three different intro-operative articular cavity perfusion treatment was given according to the randomized, double-blind and controlled rule. One way ANOVA analysis on visual analogue scale (VAS) score, functional recovery, drainage, and edema of the affected limb were performed to assess the efficiency of the treatment in the following three days after the operation.Results: Postoperative drainage in group A and B reduced significantly (P<0.05). The IHC scores of the surgical limbs were markedly lower comparing with the control group (P<0.05). No differential postoperative edema was observed and the patient acquired better rehabilitation in group A and B than control group. Compared with group A, no differential postoperative drainage and edema was seen in group B (P>0.05). The VAS score of group B was significantly lower than in group A(P<0.05). Postoperative recovery of the surgical limb function in group B was better than in group A (P<0.05), and PGE2 in the postoperative drainage volume of group B was significantly decreased (P<0.05).Conclusions: Intra-operation articular cavity perfusion therapy with iced normal saline combined with cocktail perfusion therapy can greatly reduce the early inflammation, contributing to the better rehabilitation of TKA.
Improving the electrical and optical properties of C50 fullerene using the Donor-Acceptor groups
Authors: Ayoubikaskooli Ali , Ghaedi Abdol Mohammad , Shamlouei Hamid Reza , Saghapour Yadollah 
Published: August 2021

Abstract
In this research, the C50 fullerene was employed as the source of the π electrons and the electron donor-acceptor groups were used to enhance its optical properties. Considerable enhancement in its electronic and optical property of as the result of donor and acceptor group presence was observed. For instance, in UV-Visible absorption spectrum, the number of absorption lines significantly increase which may be the relaxation of the electronic transition selection rules. Considerably, the substituted forms of C50, has numbers of absorption bands in near infrared region. The BH2–C50-NCH3Li and NO–C50-NCH3Li molecules have superior improvement in optical properties. Finally, the donor and acceptor groups influence on non-linear optical properties (NLO) of C50 were explored and the considerable improvement in NLO properties of C50 was observed which the NLO improvements for BH2-C50-NCH3Li and NO-C50-CH2Li cases is higher than others.
A Randomized Sham-controlled Trial of Manual Acupuncture for Infertile Women with Polycystic Ovary Syndrome
Authors: Pan Wen , Li Feng-xing , Wang Qiao , Yin Xiu-qi , Huang Zou-qin , Yan Yu-ming , Zhao Ling , Shen Xue-yong 
Published: August 2021

Abstract
Background: To evaluate the effectiveness of acupuncture combined with herbal medicine among the infertile female with polycystic ovary syndrome (PCOS). Method: A randomized, sham-controlled trial was conducted. A total of 86 women who were diagnosed as PCOS infertility for more than 1 year were randomly assigned to receive manual acupuncture (MA) or sham acupuncture (SA) twice per week for three menstrual cycles. Both groups received herbal medicine treatment. The evaluations were conducted at baseline, each menstruation, after three menstrual cycles and 24 weeks follow-up, including pregnancy rate, ovulation rate, sex hormones level, PCOS symptoms and Traditional Chinese Medicine (TCM) syndrome scores. Results: Among 86 randomized patients, 79 (91.86%) completed the trial. The pregnancy rate in the MA group was significantly higher than the SA group (46.34% vs 18.42%; P =0.008). The ovulation rate of the MA group was higher than the SA group (58.14% vs 45.74%; P =0.046). The improvement rate of PCOS score and testosterone level showed a statistical difference between the two groups( P <0.05), others were no significant difference between the two groups. PCOS score and TCM symptom score of the two groups were both decreased after treatment ( P <0.001). Sex hormones level including E2, T, P, LH and LH/FSH were significantly lower after intervention in the MA group ( P values were <0.05) compared with baseline, while only the progesterone level was reduced in the SA group ( P =0.008). Conclusion: Manual acupuncture combined with herbal medicine may be clinically useful for infertile women with PCOS in improving pregnancy and ovulation rate.
Fabrication of PVA/ GO Nanofiber Films by Electrospinning: Application for the Adsorption of Cu2+ and Organic Dyes
Authors: He Yao , tian huafeng , Xiang Aimin , Ma Songbai , Yin Duoyuan , A. Varada Rajulu 
Published: August 2021

Abstract
In recent years, the treatment of water pollution has been a challenging and far-reaching topic. In order to improve the adsorption performance of polyvinyl alcohol nanofibers, a new nano-adsorbent graphene oxide was introduced into them in this work. The introduction of GO greatly increases the adsorption of Cu2+, Methylene blue (MB) and Congo Red (CR). The adsorption kinetics and isotherm analysis of the PVA/GO nanofiber film during the adsorption process showed that the adsorption of Cu2+ and MB by PVA/GO nanofiber film was mainly chemical adsorption, while the adsorption of CR was mainly physical adsorption. The adsorption process of PVA/GO nanofiber film on Cu2+ was in line with the Langmuir isothermal adsorption model, that is, single molecular layer adsorption, the distribution of adsorption active sites was relatively uniform, and the increasing temperature was more conducive to the adsorption of Cu2+.
Factors Related to Information and Communication Technologies Adoption in Small Businesses in Colombia
Authors: Duran Julian , Castillo Rubén 
Published: August 2021

Abstract
This study is an analysis of how a set of factors influenced the decision to adopt information and communication technologies (ICTs) in small businesses in Colombia; for this purpose, a logistic modeling is applied wherein the explained variable represents the companies’ decision to adopt ICTs. The main explanatory elements include the demographic aspects of the owner, the company characteristics, and the legal practice of the company and its social capital. Data from the Microbusiness Survey 2019 conducted by the National Administrative Department of Statistics of Colombia were employed. The study results noted that personal aspects such as the age and gender of the owner have an impact on the adoption of ICTs. Moreover, computer equipment is critical in these small businesses, thereby making a policy of subsidizing the acquisition of these devices relevant. In addition, business formality and social capital ties have a positive influence on the use of ICTs.
Gut and Colony Microbiota of Honey Bees Apis mellifera: Social Immunity, Opportunistic Disease and Survival Overwinter.
Authors: Anderson Kirk E. , Maes Patrick 
Published: August 2021

Abstract
BackgroundOverwintering is a major contributor to honey bee colony loss and involves changes in environmental conditions, host physiology and group behavior that influence disease susceptibility. Honey bees possess a secretory head gland that interfaces with the extended colony environment on many levels, producing pro-oxidants, antioxidants and antimicrobial peptides. With the coming of winter, colonies produce a long-lived (diutinus) worker phenotype that survives until environmental conditions improve. We used a known-age worker cohort to investigate microbiome integrity and social gene expression of diutinus workers overwinter. We provide additional context by contrasting host-microbial interactions from warm outdoor and cold indoor overwintering environments. ResultsWe produce the first evidence that social immune gene expression is associated with the core hindgut and colony microbiota in honey bees, and highlight the midgut as a target of opportunistic disease overwinter. We discovered a distinct physiological and microbiological trajectory for diutinus workers that differs drastically from younger, short-lived workers in the colony. Diutinus bees were associated with decreased fungal load and decreased bacterial diversity, and increased core microbiota and longevity. Colonies overwintered indoors maintained a stable or improved microbiota structure and complimentary gene expression overwinter. In contrast, workers from colonies overwintered outdoors in warm southern conditions possessed changes co-occurring throughout the alimentary tract microbiota that suggest opportunistic disease progression and resistance in diutinus workers, but susceptibility to opportunistic disease in younger workers that emerged during the winter, including increases in Enterobacteriaceae, fungal load and bacterial diversity abundance. ConclusionsOur results highlight social selection pressures that shaped the colony and hindgut microbiome with evolution to a perennial life history. The results are consistent with a “group level” explanation of social immunity, including host associations with the colony microbiota, and a social immune response by long-lived diutinus workers to accompany microbial opportunism. The cost/benefit ratio associated with limited expression of the diutinus phenotype may be a strong determinant of colony survival overwinter. The relationship of colony and gut microbiota with social immune function highlights the range of host-microbial interaction associated with the honey bee superorganism, and its potential influence on colony health, disease resistance and gut integrity.
How to Make Online Mood-monitoring in Bipolar Patients a Success? A Qualitative Exploration of Requirements.
Authors: Geerling Bart , Kelders Saskia M. , Kupka Ralph W. , Stevens Anja W.W.M. , Bohlmeijer Ernst T. 
Published: August 2021

Abstract
Background The Life-Chart Method (LCM) is an effective self-management treatment option in bipolar disorder (BD). There is insufficient knowledge about the consumers' needs and desires for an e-monitoring solution. The first step towards a new mood monitoring application is an extended inventory among consumers and professionals.BackgroundThe aim of the current study was: to identify opinions about online mood monitoring of patients with BD and professionals and to identify preferences on design, technical features and options facilitating optimal use and implementation of online mood monitoring.This study used a qualitative design with focus groups. Participants were recruited among patients and care providers. Three focus groups were held with eight consumers and five professionals. ResultsThe focus group meetings reveal a shared consciousness of the importance of using the Life-Chart Method for online mood monitoring. There is a need for personalization, adjustability, a strict privacy concept, an adjustable graphic report, and a link to early intervention strategies in the design. Due to the fact that this is a qualitative study with a relative small number of participants, so it remains unclear whether the results are fully generalizable. We can’t rule out a selection bias.Conclusions This study demonstrates the importance of involving stakeholders in identifying a smartphone-based mood charting applications' requirements. Personalization, adjustability, privacy, an adjustable graphic report, and a direct link to early intervention strategies are necessary requirements for a successful design. The results of this value specification are included in the follow-up of this project.
The determination of high retention flag marking methods with yellowfin bream (Acanthopagrus latus) fry
Authors: Chen Po-Cheng , SHIH CHUN-HAN , Wang Jia-Jiao , Huang Liang-Ming 
Published: August 2021

Abstract
With the decline in coastal fishery resources, hatchery-reared fry of yellowfin bream (Acanthopagrus latus) have been commonly mass released to the surrounding waters in China in recent years. Although the release plan has been underway in China for many years, the releasing effects were not often assessed. Therefore, it is necessary to study several suitable mass marking methods that have a high mark retention rate for fry. From October to November 2020, tetracycline was immersed to mark calcified hard tissue (MI), with tagging cut tail fin (TC) and the control, and the species of yellowfin bream by live fish were examined. Moreover, a double marking method, i.e., mark calcified hard tissue (MI) after tag fish for two weeks, was examined to determine any increase in the mark retention rate for yellowfin bream in November 2020. The dual marking and tagging method combining MI and TC is effective and is suitable for yellowfin bream. Both MI of 0.25% treatment and MI of 0.5% treatment also have lower costs, and more individuals can be marked.
Single cell transcriptome identifies molecular subtype of autism spectrum disorder impacted by de novo loss of function mutation genes regulating glial cells
Authors: Nassir Nasna , Bankapur Asma , Samara Bisan , Ali Abdulrahman , Ahmed Awab , Inuwa Ibrahim , Zarrei Mehdi , Shabestari Seyed Ali Safizadeh , AlBanna Ammar , Howe Jennifer L. , Berdiev Bakhrom , Scherer Stephen W. , Woodbury-Smith Marc , uddin mohammed 
Published: August 2021

Abstract
Background

In recent years, several hundred autism spectrum disorder (ASD) implicated genes have been discovered impacting a wide range of molecular pathways. However, the molecular underpinning of ASD, particularly from the point of view of ‘brain to behaviour’ pathogenic mechanisms, remains largely unknown.
Methods

We undertook a study to investigate patterns of spatiotemporal and cell type expression of ASD-implicated genes by integrating large-scale brain single cell transcriptomes (> million cells) and de novo loss of function (LOF) ASD mutations (impacting 852 genes from 40122 cases).
Results

We identified multiple single cell clusters from three distinct developmental human brain regions (anterior cingulate cortex, middle temporal gyrus and primary visual cortex) that evidenced high evolutionary constraint through enrichment for brain critical exons and high PLi genes. These clusters also showed significant enrichment with ASD loss of function mutation genes (p < 5.23 x 10− 11) that are transcriptionally highly active in prenatal brain regions (visual cortex and dorsolateral prefrontal cortex). Mapping ASD de novo LOF mutated genes into large scale human and mouse brain single cell transcriptome analysis demonstrate enrichment of such genes into neuronal subtypes and are also enriched for subtype of non-neuronal glial cell types (astrocyte, p < 6.40 x 10− 11; oligodendrocyte, p < 1.31 x 10− 09).
Conclusion

Among the ASD genes enriched with pathogenic de novo LOF mutations (i.e., KANK1, PLXNB1), a subgroup has restricted transcriptional regulation in non-neuronal cell types that are evolutionarily conserved. This association strongly suggests the involvement of subtype of non-neuronal glial cells in the pathogenesis of ASD, and the need to explore other biological pathways for this disorder.
Development and Evaluation of Droplet Digital PCR Assay for the Detection of Reticuloendotheliosis Virus in Attenuated Vaccines
Authors: Meng Fanfeng , Ren Zhihao , Wang Yixin , Zhao Peng , Zhang Guozhong 
Published: August 2021

Abstract
Background: The use of Reticuloendotheliosis virus (REV) from contaminated live virus vaccine is suspected to be one of the most important causes of massive outbreaks of Reticuloendotheliosis in China. Methods: In this study, we established a droplet digital PCR (ddPCR) detection method for REV and compared its sensitivity to different methods to detect REV contamination in a vaccine. Results: The results indicated that both quantitative PCR and dot-blot methods could detect REV contamination at a dose of 1 TCID50/1,000 feathers, whereas ddPCR could detect REV contamination at a dose of 0.1 TCID50/1,000 feathers, which is 1,000-fold more sensitive than conventional polymerase chain reaction detection (102 TCID50/1000 feathers). ddPCR not only exhibited the highest sensitivity but also proved extremely intuitive, especially to detect REV contamination in vaccines.Conclusions: The ddPCR method established in this study to detect REV contamination in vaccines can effectively detect and quantify low-dose REV contamination. This provides a new method for the rapid detection of REV contamination in various samples, especially vaccines.
Assessing the Effect of Public Transfer Payments on the Vulnerability of Health Care Poverty in Rural Households in China
Authors: Li Yali 
Published: August 2021

Abstract
Background

Since the 18th National Congress, China has completed the task of eliminating absolute poverty. However, the risk of health care for potential sub-health workers and the elderly in rural households has also increased, and government public transfer payments play an important role in reducing the vulnerability of rural families to health care poverty. Assessing the effectiveness of government public transfer payments in reducing the vulnerability of rural families to health care poverty will help the public sector to develop more accurate policies to meet the different needs of rural households for health care.
Methods

The data were derived from the 3rd 、4th and 5th National Survey of the China Family Panel Studies conducted in 2010. The annual study sample was 5,574 rural households, with a total sample of 16,722 rural households during the three-year study period. We assess and compare the multidimensional poverty and the vulnerability to health care poverty of rural households. Two series of multivariate logistic regression models were further undertaken to assess the effect of government public transfer payments before and after adding the income elasticity of health care needs on improving the vulnerability of rural families to health care poverty.
Results

The study found that when setting the poverty line at $1.90 and $3.20, rural households in the three research years expected a higher vulnerability to health care poverty than their actual incidence of multidimensional poverty in health care, and higher poverty vulnerability in the eastern region than in the western region. A series of multivariate logistic models evaluated the effect of government public transfer payment on improving the vulnerability of rural health care poverty and found that it is difficult for the government to fulfill the effect of public transfer payment without considering the difference of rural households' demand for health care.
Conclusions

At present, the imbalance of development between urban and rural areas in China is increasing, and rural families with heavy economic burdens are facing the risk of sub-health such as potential chronic illness. Our analysis emphasizes the importance of government departments in improving the targeting of public transfer payments to reduce the vulnerability of health care poverty in rural households.
Xijiao Dihuang Decoction Alleviates Lung Injury Induced by Sepsis Through Regulating Glycerinpho Spholipid Metabolism Based Lipidomics
Authors: yu Chen Huai , rui Lin Ming , Dan Wen , feng Su Jun , Jie Yao , li Zhu Xue , Wei Li 
Published: August 2021

Abstract
Background and Objective: To observe the effect of Xijiao Dihuang Decoction (XJDHT) on lung injury of sepsis mice with lipidomic analysis, and explore the underlying mechanisms.Materials and Methods: C57BL/6 mice were induced sepsis by lipopolysaccharide (LPS) administration. Animals are pretreated with XJDHT 2 days before LPS regimen, then continue treatment for addition 3 days. Lung tissue are collected for lipidomic analysis by LC/MS/MS. The survival rate is monitor after treatment. Results: After treatment with XJDHT, the histological changes of lung of the sepsis mice were relieved. The lipidomic profiles of the lung in sepsis group were evidently disordered when compared to the control group. A total of 40 significantly differential lipids expression between the sepsis-control groups were identified by multivariate statistical analysis. Septic lung injury may result from such lipid disorders, which are related to fatty acyls metabolism, glycerophospholipid metabolism, phosphosphingolipids metabolism and glycerides metabolism. After XJDHT treatment, the lipidomic profiles of the disorders tend towards alleviated when compared to the sepsis group. Twelve lipid molecules were identified and some glycerophospholipids levels returned close to the normal level. Conclusion: Glycerophospholipid metabolism may play an important role in the treatment of septic lung injury using XJDHT.
Safety of High-Dose Vitamin C in Critically Ill Patients: A Systematic Review and Meta-analysis
Authors: Shrestha Dhan Bahadur , Budhathoki Pravash , Sedhai Yub Raj , Mandal Sujit Kumar , Shikhrakar Shreeja , Karki Saurab , Baniya Ram Kaji , Kashiouris Markos G. , Qiao Xian , Fowler Alpha A. 
Published: August 2021

Abstract
Objective:High-Dose Intravenous Vitamin C (HDIVC) is currently a controversial therapy for sepsis and ARDS. The published evidence regarding its efficacy in critically ill patients has shown conflicting results, and in fact, case reports have raised concerns for nephrotoxicity. The objective of this meta-analysis is to critically appraise the latest evidence regarding the safety of HDIVC in critically ill patients.Data Sources: Structured literature search on PubMed, PubMed Central, Scopus, Embase, and Google Scholar.Study Selection: Cross-sectional studies, case-control studies, cohort studies, randomized controlled trials, and case series with 20 or more critically ill patients who have received intravenous ascorbic acid (vitamin C), published till February 25, 2021. We identified 53 studies in our qualitative analysis and 48 studies in our quantitative analysis among a standardized search of 18,312 studies.Data Synthesis:We pooled data and calculated Odds Ratios (OR) and Mean Differences (MD) with their 95% confidence intervals to assess explanatory variables. Based on a random effect model from 33 studies, pooled hospital mortality outcomes showed a 19% reduction in odds for overall hospital mortality among the HDIVC group (OR, 0.81; 95% CI, 0.66-0.98). Mortality at 28/30-days, ICU mortality, length of hospital stay (LOS), new-onset AKI, and Renal Replacement Therapy (RRT) for AKI did not differ significantly across treatment and control groups. Pooled data from 30 studies disclosed 0.76 fewer ICU days in the HDIVC group than the placebo/ standard of care (SOC) group, 95% CI, -1.34 to -0.19. This significance persisted when we included RCTs only in the analysis (MD, -0.70; 95% CI, -1.39 to -0.02).Conclusions:Our results suggest that HDIVC treatment is renally safe and did not increase adverse kidney events, or mortality. It was associated with a slight reduction in ICU length of stay in critically ill patients.