bluuebunny/crossref_metadata_2025_split huggingface dataset:
Myotonic dystrophy RNA toxicity alters morphology, adhesion and migration of mouse and human astrocytes
Authors: Dincã Diana , González-Barriga Anchel , Sicot Geraldine , Lallemant Louison , Pillet Laure-Elise , Cresto Noémie , Braz Sandra , Polveche Helene , Huguet Aline , Azotla-Vilchis Cuauhtli N. , Agonizantes-Juárez Luis , Tahraoui-Bories Julie , Martinat Cecile , Hernández-Hernández Oscar , Auboeuf Didier , Rouach Nathalie , Bourgeois Cyril F , Gourdon Genevieve , Gomes-Pereira Mário 
Published: October 2021

Abstract
Brain dysfunction in myotonic dystrophy type 1 (DM1), the prototype of toxic RNA disorders, has been mainly attributed to neuronal RNA misprocessing, while little attention has been given to non-neuronal brain cells. Using a transgenic mouse model of DM1 that expresses mutant RNA in various brain cell types, we demonstrate that astrocytes exhibit impaired ramification and polarization in vivo and defects in adhesion, spreading and migration. RNA-dependent toxicity and phenotypes was also found in human transfected glial cells. In line with the cell phenotypes, molecular analyses revealed extensive expression and accumulation of toxic RNA in astrocytes, which resulted in RNA spliceopathy that was remarkably more severe than in neurons. Astrocyte missplicing affected primarily transcripts that regulate cell adhesion, cytoskeleton and morphogenesis, and it was confirmed in human brain tissue. We demonstrate for the first time that DM1 impacts astrocyte cell biology, possibly compromising their support and regulation of synaptic function.
Influence of Kappaphycus Alvarezii and Gracilaria Salicornia Supplementation on in Vitro Fermentation Pattern, Total Gas and Methane Production of Mixed Substrates
Authors: Munde Vithal K. , Das Asit , Singh Putan , Verma Ashok K. , Muwel Nirmala , Mishra Alok , Deb Rajib , Raje Kanti 
Published: October 2021

Abstract
This experiment was conducted to study the effect of supplementation of Kappaphycus alvarezii (KA) and Gracilaria salicornia (GS) in vitro fermentation pattern, total gas and methane production of mixed substrates. Basal substrate comprising of concentrates and wheat straw (50:50) was supplemented with either 0% (control), 1 (KA1), 2 (KA2), 4 (KA4), 6 (KA6), and 8 % (KA8) of Kappaphycus; and, 1 (GS1), 2 (GS2), 4 (GS4), 6 (GS6), and 8 (GS8 ) of Gracilaria, respectively. Asymptote, rate constant of gas production and t-half, concentration of total volatile fatty acids (TVFA), and in vitro dry matter digestibility (IVDMD) was not affected up to 2% level KA supplementation, beyond which asymptote, and rate constant of gas production, TVFA, and IVDMD decreased and t-half increased (P<0.001). Asymptote, rate constant of gas production, TVFA and IVDMD was not affected at 1% level of inclusion, beyond which a steady decline in these parameters was observed (P<0.001). Methane production (ml/g DM) was higher (P<0.001) in CON, followed by KA1 and KA2, and lower values were observed in by KA4, KA6 and KA8. Methane production (ml/kg DM) declined (P<0.001) steadily with increased level of GS in the substrates. From the results it was concluded that inclusion of Kappaphycus alvarezii and Gracilaria salicornia at 2 and 1%, respectively in the fermentation substrate can reduce in vitro methane production without any adverse impact on total gas production and in vitro dry matter digestibility.
Therapeutic STING activation boosts microglia phagocytosis and ameliorates depression-like behaviors during chronic restraint stress
Authors: Zhai Qian , Zhang Yanpeng , Tan Shuwen , Sun Jianyu , Ye Mao , Gao Hui , Pu Kairui , Wu Meiyan , Wang Qiang 
Published: October 2021

Abstract
Background
The STING-TBK1-IRF3 signaling pathway involves in modulating host innate immunity, however, the potential role of STING signaling pathway in chronic restraint stress model has not been determined. The aim of this study is to explore the underlying role of STING signaling pathway in regulating neuroinflammation, as well as to evaluate the therapeutic potential of STING agonist during chronic restraint stress.
Methods
C57BL/6 mice were subjected to 14-day intermittent restraint stress. Sucrose preference, elevated plus maze and tail suspension tests were measured in chronic restraint stress mice. Expression levels of proinflammatory cytokines were tested by QT-PCR and Luminex cytokine assays. The fluorescence-labeled latex beads, flow cytometry and CD68 positive cell counts were utilized to evaluate phagocytic abilities of microglia. Then, the ability of intracerebroventricular injection of STING agonist, 2’3-cGAMP, to reverse the depression-like behaviors and inflammatory cytokines was examined.
Results
We found that the expression levels of STING, p-TBK1, and p-IRF3 were remarkably decreased in chronic restraint stress mice, which was associated with decreased IFN-β secretion. Moreover, the STING agonist, 2’3-cGAMP, significantly alleviated the neuroinflammation and ameliorated depression-like behavior which depends on the functional STING activation. Furthermore, 2’3-cGAMP promoted microglia phagocytosis through STING-dependent IFN-β release, which was essential for recovery from neuroinflammation during chronic restraint stress.
Conclusions
These findings demonstrate that STING signaling pathway is a critical mediator in regulating microglia phagocytosis and may serve as a novel therapeutic target for chronic stress-related psychiatric diseases.
Determination of The Relationship Between Exposure To Novel Industrial Toxicant HFPO-DA, C. Elegans, and Aging
Authors: Nagam Vishruth Mohan 
Published: October 2021

Abstract
Industrial toxicant hexafluoropropylene oxide dimer acid (HFPO-DA, ammonium salt with trade name “GenX”) has recently been detected in the environment. HFPO-DA’s potential aging-related effects on organisms of higher trophic levels (worms, humans, etc.) have not been extensively explored. This study aims to quantify impact on C. elegans (free-living nematode) lifespan of HFPO-DA exposure, specifically via ingestion to simulate toxicant exposure mechanisms most prevalent in nature (through lower trophic-level organisms). C. elegans N2 (wild-type) samples were prepared with uracil-based medium and E. coli OP50 (food source) at room temperature; C. elegans in the experimental, treated sample was fed E. coli OP50 incubated with 280 ng/L HFPO-DA. The target gene pqm-1 was selected due to its key role in an aging-related insulin signaling pathway evolutionarily conserved in humans. Molecular biology laboratory techniques (RNA extraction, qRT-PCR, fluorescence tagging, etc.) were used to quantify pqm-1 expression to yield four technical replicates for each sample. The data was analyzed through null hypothesis t-tests, heatmaps, protein interactions, and gene homology tools. HFPO-DA exposure through E. coli caused a statistically insignificant (0.811-fold) change in pqm-1-related aging in C. elegans. Future work includes investigating the effects of different levels of HFPO-DA exposure on C. elegans aging.
Experimental analysis on the optimal excitation wavelength for fine-grained identification of refined oil pollutants on water surface based on laser-induced fluorescence
Authors: Xie Ming , Jia Yunpeng , Li Ying , Cai Xiaohua , Cao Kai 
Published: October 2021

Abstract
Laser-induced fluorescence (LIF) is an effective, all-weather oil spill identification method that has been widely applied for oil spill monitoring. However, the distinguishability on oil types is seldom considered while selecting excitation wavelength. This study is intended to find the optimal excitation wavelength for fine-grained classification of refined oil pollutants using LIF by comparing the distinguishability of fluorometric spectra under various excitation wavelengths on some typical types of refined-oil samples. The results show that the fluorometric spectra of oil samples significantly vary under different excitation wavelengths, and the four types of oil applied in this study are most likely to be distinguished under the excitation wavelengths of 395 nm and 420 nm. This study is expected to improve the ability of oil types identification using LIF method without increasing time or other cost, and also provides theoretical basis for the development of portable LIF devices for oil spill identification.
Open-Source Software Library For Real-Time Inertial Measurement Unit Data-Based Inverse Kinematics Using OpenSim
Authors: Lavikainen Jere , Vartiainen Paavo , Stenroth Lauri , Karjalainen Pasi 
Published: October 2021

Abstract
An open-source software library for multithreaded real-time inverse kinematical (IK) analysis of inertial measurement unit (IMU) data using OpenSim was developed. Its operation delays and throughputs were measured with a varying number of IMUs and parallel computing IK threads using two different musculoskeletal models, one a lower-body and torso model and the other a full-body model. Full-body inverse kinematics with data from 12 IMUs could be calculated in real-time with a mean delay below 100 ms and at more than 900 samples per second. Live visualization of IK is an option but results in limited IK throughput. The effect of this limitation was assessed by comparing the range of motion (ROM) of each joint from visualized real-time IK to the ROM from offline IK at IMU sampling frequency, resulting in mean ROM differences below 0.3 degrees. The software library enables real-time inverse kinematical analysis with different numbers of IMUs and customizable musculoskeletal models, making it possible to do subject-specific full-body motion analysis outside the motion laboratory in real-time.
Artesunate Inhibits the Development of PVR by Supreesing TGF-β/Smad Signaling Passway
Authors: XU Qihua , Wang Zi-Yi 
Published: October 2021

Abstract
Proliferative vitreoretinopathy (PVR) is the main reason for the failure of retinal detachment surgeryEpithelial-mesenchymal transition (EMT) induced by transforming growth factor (TGF-β2) plays an important role in the development of PVR.Artesunate has been widely studied in the treatment of ophthalmic diseases because of its antioxidant, anti-inflammatory, anti-apoptosis and anti-proliferation effects.The purpose of this study was to investigate the effect of artesunate on EMT induced by TGF-β2 in ARPE-19 cells and its effect on PVR processWe found that artesunate can inhibit the proliferation of ARPE-19 cells after EMT transformation, inhibit the contraction of ARPE-19 cells after EMT transformation, and inhibit the autocrine of TGF-β2 in ARPE-19 cellsWe also found that the contents of Smad3 and p-smad3 in clinical samples increased,Artesunate can inhibit the contents of Smad3 and p-smad3 in ARPE-19 cells induced by TGF-β2Artesunate can inhibit the occurrence and development of PVR diseases in vivoTo sum up, artesunate can inhibit the occurrence and development of PVR diseases by inhibiting the EMT process of ARPE-19 cells.
Characteristics of Step Responses Following Varying Magnitudes of Unexpected Lateral Perturbations During Standing Among Older People– Cross-sectional Laboratory Based Study
Authors: Batcir Shani , Shani Guy , Shapiro Amir , Melzer Itshak 
Published: October 2021

Abstract
Introduction—The inability to recover from unexpected lateral loss of balance may be particularly relevant to the problem of falls.Aim—We aimed to explore whether different kinematic patterns and strategies occur in the first recovery step in single-step trials when single step was required to recover from fall and in the multiple-step trials, when more than one step were required to recover from fall. In addition, in the multiple-step trials we examined kinematic patterns of balance recovery where extra steps were needed to recover balance. Methods—Eighty-four older adults (79.3±5.2 years) were exposed to announced right/left perturbations in standing that were gradually increased to trigger a recovery stepping response. We performed kinematic analysis of the first recovery step of all single-step and multiple-step trials for each participant and of total balance recovery in the multiple-step trial.Results—Kinematic patterns and strategies of the first recovery step in the single-step trials were significantly dependent on the perturbation magnitude. There was a very small, yet significant increase in timing of recovery step as the magnitude increased. In contrast, the first recovery step of the multiple-step trials showed no significant differences between different perturbation magnitudes; while, in total balance recovery of these trails, we observe a small yet significant difference as the magnitude increased. Conclusions—The first recovery step of the single-step trials i.e., a relatively low perturbation magnitudes, different kinematic movement patterns were used as perturbation magnitudes increased, suggesting that older adults pre-plan their stepping performance. However, in the first recovery step of the multiple-step trials i.e., high perturbation magnitudes, similar kinematic movement patterns were used at different magnitudes, suggesting a more stereotypical behavior.
Regression Analysis On Ground Motion Parameters For The Earthquakes (Mw≥4.0) in NE India With Special Emphasis On 3 Jan 2016 M6.7 Tamenglong Earthquake
Authors: Gogoi Ashim , Baruah Saurabh , Sharma Sangeeta 
Published: October 2021

Abstract

In the present study, ground motion parameters are estimated by processing 26 number of Earthquake events which contain 128 accelerogram records with 384 components of earthquakes that originated in NE, India and its vicinity with special emphasis on 3 Jan 2016 M6.7, Tamenglong Earthquake. It is observed that the peak ground acceleration (PGA) is dependent on distance, site conditions, and site characteristics. The linear regression relations developed for GMPs with distance can be applied to characterize sites having three kinds of geology- Quaternary, Tertiary, and Precambrian. Attenuation relation curves of ground motion parameters (PGA, PGV, Ia, Ic, CAV, HI, EDA, ASI) of three different magnitudes (Mw=5.4, 5.5, and 5.8) are estimated for NS and EW components. The average response acceleration of horizontal components for all these sites is equivalent to 0.0485g for 5% damping with reference to the average period of 0.234s and for the vertical component is around 0.026g to the average period of 0.18s. January 3, 2016, Mw=6.7, Tamenglong earthquake, Manipur is one of the highest magnitudes in NER used in this study. Estimated Peak Ground Acceleration (PGA) at Itanagar, Jorhat, Kohima, Lekhapani, Mokokchung and Tadong sites are 0.0289g, 0.0145g, 0.053g, 0.00510g, 0.022g, 0.00159g respectively, while the Peak Ground Velocity (PGV) values are 0.0166m/s, 0.0097m/s, 0.050m/s, 0.0022m/s, 0.0195868m/s, 0.0009498m/s respectively. The PGA estimated for the event is found to be maximum at Kohima. Response spectra indicate that response acceleration mostly varies with periods 0.2s and 0.5s, and beyond 2s there is no effective response acceleration. The maximum effective response accelerations for all the sites are observed within the frequency range of 0.5 Hz to 5 Hz. Pseudo response acceleration (0.2802g at 1.72 Hz) along with estimated engineering parameters was observed to be higher in Kohima site situated on hard rock with a low epicentral distance of about 120 km. These relations help to forecast an average value for the GMPs estimated at a particular site at a certain distance from a source which is a prime input for seismic hazard analyses and the development of design ground motions.
Associating Sleep Problems With Advanced Cancer Diagnosis, and Immune Checkpoint Treatment Outcomes: a Pilot Study
Authors: Sillah Arthur , Peters Ulrike , Watson Nathaniel F. , Tykodi Scott S. , Hall Evan T. , Silverman Allison , Malen Rachel C , Thompson John A. , Lee Sylvia M. , Bhatia Shailender , Veatch Joshua , Warner Jeannie , Thornton Timothy , Phipps Amanda I. 
Published: October 2021

Abstract
Background: Sleep problems (SP) are common in cancer patients but have not been previously assessed in patients receiving immune checkpoint inhibitors (ICI).Methods: We collected questionnaire data on sleep apnea risk, insomnia and general sleep patterns. We used an adjusted multivariate Poisson regression to calculate prevalence ratios (PRs) and associated 95% confidence intervals (CIs) for associations between these SP and metastatic versus localized cancer stage (M1 vs M0), and adjusted logistic regression models to calculate ORs for associations between SP with the number of ICI infusions completed (6+ vs. <6).Results: Among 32 patients who received ICI treatment, the prevalence of low, intermediate, and high-risk OSA risk was 36%, 42%, and 21%, respectively. Overall, 58% of participants reported clinically significant insomnia. We did not find a significant association between intermediate or high risk OSA (vs. low risk) and metastatic cancer status [PR=1.01 (95% CI: 0.28, 3.67)]. Patients in the cohort who reported taking >15 minutes to fall asleep were 3.6 times more likely to be diagnosed with metastatic cancer compared to those reporting shorter sleep latency [95% CI (1.74, 7.35)]. We did not find a significant association between SP and number of ICI infusions completed.Conclusion: Our data associating sleep apnea risk, insomnia and sleep patterns with more advanced cancer encourages further exploration in larger-scale observational studies and suggests interventional clinical trials focused on sleep quality improvement could result in better outcomes for these patients.
Prediction of death after multiple wasp stings with a clinical prognostic model
Authors: Wang Maohe , Qin Mei , Wang Amanda Y , Zhao Jia Wei , Deng Fei , Li Guisen , Wang Wei 
Published: October 2021

Abstract
Background
We aimed to assess the utility of the poisoning severity score (PSS) as early prognostic predictors in patients with wasp stings, and to explore a reliable and simple predictive tool for short-term outcomes.
Methods
From January 2016 to December 2018, 363 patients with wasp stings in Suining Central Hospital were taken as research subjects. In the first 24h of hospital admission, the PSS and Chinese expert consensus on standardized diagnosis and treatment of wasp stings (CECC) were used as the criterion for severity classification, and their correlation was analyzed. The patients were divided into survival and death groups according to the state of discharge. The factors that affect outcome were analyzed by logistic regression analysis. A clinical prognostic model of death was constructed according to the risk factors, and 1000 times repeated sampling was done to include the data to verify the model internally.
Results
The mortality of wasp sting patients was 3.9%. There was a correlation between PSS and CECC (r=0.435, P<0.001) for severity classification. Sex, age, number of stings, and PSS were independent risk factors for death. Based on the 4 independent risk factors screened by the above regression analysis, a nomogram model was constructed to predict the risk of death in wasp sting patients. The predicted value C-index was 0.962, and the internally verified AUC was 0.962(95%C.I. 0.936-0.988, P<0.001).
Conclusions
PSS is helpful in the early classification of the severity of wasp stings. Sex, age, number of stings, and PSS were independent risk factors for death in wasp sting patients. The nomogram model established in this study can accurately predict the occurrence of the risk of death.
CEST MRI Provides Amides/amines Surrogate Biomarkers for Treatment-naïve Glioma Sub-typing
Authors: Mancini Laura , Casagranda Stefano , Gautier Guillaume , Peter Philippe , Lopez Bruno , Thorne Lewis , McEvoy Andrew , Miserocchi Anna , Samandouras George , Kitchen Neil , Brandner Sebastian , De Vita Enrico , Torrealdea Francisco , Rega Marilena , Schmitt Benjamin , Liebig Patrick , Sanverdi Eser , Golay Xavier , Bisdas Sotirios 
Published: October 2021

Abstract
PurposeAccurate gliomas classification affects patient management and is challenging on non- or low-enhancing gliomas. This study investigated the clinical value of different Chemical Exchange Saturation Transfer (CEST) metrics for glioma classification and assessed the diagnostic effect of the presence of abundant fluid in gliomas subpopulations.MethodsForty-five treatment-naïve glioma patients with known isocitrate dehydrogenase (IDH) mutation and 1p/19q codeletion status received CEST MRI at 3T. Magnetisation transfer ratio asymmetry and CEST metrics (amides: offset range 3-4ppm, amines: 1.5-2.5ppm, amides/amines ratio) were calculated with two models: ‘asymmetry-based’ (AB) and ‘fluid-suppressed’ (FS). Presence of T2/FLAIR mismatch was noted.ResultsIDH-wildtype had higher amides/amines ratio than IDH-mutant_1p/19qcodel (p<0.022). Amides/amines ratio and amines levels differentiated IDH-wildtype from IDH-mutant (p<0.0045) and from IDH-mutant_1p/19qret (p<0.021). IDH-mutant_1p/19qret had higher amides and amines than IDH-mutant_1p/19qcodel (p<0.035). IDH-mutant_1p/19qret with AB/FS mismatch had higher amines than IDH-mutant_1p/19qret without AB/FS mismatch (p<0.016). In IDH-mutant_1p/19qret, the presence of AB/FS mismatch was closely related to the presence of T2/FLAIR mismatch (p=0.014).ConclusionsCEST-derived biomarkers for amides, amines and their ratio can help with histomolecular staging in gliomas without intense contrast enhancement. T2/FLAIR mismatch is reflected in the presence of AB/FS CEST mismatch. The AB/FS CEST mismatch identifies glioma sub-groups that may have prognostic and clinical relevance.
Modification of the Luedeking and Piret Model with a Delay Time Parameter for Biotechnological Lactic Acid Production
Authors: Groff María Carla , Scaglia Gustavo , Ortiz Oscar A. , Noriega Sandra E. 
Published: October 2021

Abstract
Objectives
To obtain a mathematical model that adequately describes the time lag between biomass generation and lactic acid production of lactic fermentations.
Methods
Seven experimental kinetics from other research works were studied to validate our proposal: four studies of Fungal Submerged Fermentation and three cases of Bacterial Submerged Fermentation, including the data recollected by Luedeking and Piret.
Results
We introduce a modification to the Luedeking and Piret model that consist in the introduction of a time delay parameter in the model, this parameter would account for the lag time that exists between the production of biomass and lactic acid. It is possible to determine this time delay in a simple way by approximating the biomass and product formation considering that they behave as a first order plus dead time system. The duration of this phenomenon, which is not described with the classical Luedeking and Piret model, is a function of microorganism physiology (ease of biomass growth), environment (nutrients) and type of inoculum.
Conclusion
The Luedeking and Piret with delay model applications reveal an increase of the R2 in all cases, evidencing the quality of fit and the simplicity of the method proposed. These model would improve the accuracy of bioprocess scaling up.