bluuebunny/crossref_metadata_2025_split huggingface dataset:
A novel prognostic signature of immune-related lncRNA pairs in lung adenocarcinoma
Authors: Liu Yang , Wu Qiuhong , Fan Xuejiao , Li Wen , Li Xiaogang , Zhu Hui , Zhou Qinghua , Yu Jinming 
Published: August 2021

AbstractLung adenocarcinoma (LUAD) is the most common subtype of lung cancer, but the prognosis of LUAD patients remains unsatisfactory. Here, we retrieved the RNA-seq data of LUAD cohort from The Cancer Genome Atlas (TCGA) database and then identified differentially expressed immune-related lncRNAs (DEirlncRNAs) between LUAD and normal controls. Based on a new method of cyclically single pairing along with a 0-or-1 matrix, we constructed a novel prognostic signature of 8 DEirlncRNA pairs in LUAD with no dependence upon specific expression levels of lncRNAs. This prognostic model exhibited significant power in distinguishing good or poor prognosis of LUAD patients and the values of the area under the curve (AUC) were all over 0.70 in 1, 3, 5 years receiver operating characteristic (ROC) curves. Moreover, the risk score of the model could serve as an independent prognostic factor for patients with LUAD. In addition, the risk model was significantly associated with clinicopathological characteristics, tumor-infiltrating immune cells, immune-related molecules and sensitivity of anti-tumor drugs. This novel signature of DEirlncRNA pairs in LUAD, which did not require specific expression levels of lncRNAs, might be used to guide the administration of patients with LUAD in clinical practice.
Preparation of siRNA–PLGA/Fabʹ–PLGA mixed micellar system with target cell-specific recognition
Authors: Hazekawa Mai , Nishinakagawa Takuya , Mori Takeshi , Yoshida Miyako , Uchida Takahiro , Ishibashi Daisuke 
Published: August 2021

AbstractSmall interfering RNAs (siRNAs) are susceptible to nucleases and degrade quickly in vivo. Moreover, siRNAs demonstrate poor cellular uptake and cannot cross the cell membrane because of its polyanionic characteristics. To overcome these challenges, an intelligent gene delivery system that protects siRNAs from nucleases and facilitates siRNA cellular uptake is required. We previously reported the potential of siRNA-poly(d,l-lactic-co-glycolic acid; PLGA) micelles as an effective siRNA delivery tool in a murine peritoneal dissemination model by local injection. However, there was no effective formulation for siRNA delivery to target cells via intravenous injection. This study aimed to prepare siRNA–PLGA/Fabʹ–PLGA mixed micelles for siRNA delivery to target floating cells and evaluate its formulation in vitro. As the target siRNA protein in CEMx174, CyclinB1 levels were significantly reduced when siRNA–PLGA/Fabʹ–PLGA mixed micelles were added to cells compared with siRNA–PLGA micelles. siRNA–PLGA/Fabʹ–PLGA mixed micelles have high cell permeability and high target cell accumulation by endocytosis because flow cytometry detected labeling micelles in target cells. This study supports siRNA–PLGA/Fabʹ–PLGA mixed micelles as an effective siRNA delivery tool. This formulation can be administered systemically in dosage form against target cells, including cancer metastasis or blood cancer.
Reusable surface amplified nanobiosensor for the sub PFU/mL level detection of airborne virus
Authors: Shin Junghyun , Kim Hyeong Rae , Bae Pan Kee , Yoo Haneul , Kim Jeongsu , Choi Yoonji , Kang Aeyeon , Yun Wan S. , Shin Yong Beom , Hwang Jungho , Hong Seunghun 
Published: August 2021

AbstractWe developed a reusable surface-amplified nanobiosensor for monitoring airborne viruses with a sub-PFU/mL level detection limit. Here, sandwich structures consisted of magnetic particles functionalized with antibodies, target viruses, and alkaline phosphatases (ALPs) were formed, and they were magnetically concentrated on Ni patterns near an electrochemical sensor transducer. Then, the electrical signals from electrochemical markers generated by ALPs were measured with the sensor transducer, enabling highly-sensitive virus detection. The sandwich structures in the used sensor chip could be removed by applying an external magnetic field, and we could reuse the sensor transducer chip. As a proof of concepts, the repeated detection of airborne influenza virus using a single sensor chip was demonstrated with a detection limit down to a sub-PFU/mL level. Using a single reusable sensor transducer chip, the hemagglutinin (HA) of influenza A (H1N1) virus with different concentrations were measured down to 10 aM level. Importantly, our sensor chip exhibited reliable sensing signals even after more than 18 times of the repeated HA sensing measurements. Furthermore, airborne influenza viruses collected from the air could be measured down to 0.01 PFU/mL level. Interestingly, the detailed quantitative analysis of the measurement results revealed the degradation of HA proteins on the viruses after the air exposure. Considering the ultrasensitivity and reusability of our sensors, it can provide a powerful tool to help preventing epidemics by airborne pathogens in the future.
Calcium silicate-based cements cause environmental stiffness and show diverse potential to induce osteogenesis in human osteoblastic cells
Authors: Santiago Marcos Coelho , Gomes-Cornélio Ana Lívia , de Oliveira Laudimar Alves , Tanomaru-Filho Mario , Salles Loise Pedrosa 
Published: August 2021

AbstractCalcium silicate-based cements differ markedly in their radiopacifiers and the presence of calcium sulfate, aluminates, carbonates and other components that can affect their biological properties. This study aimed to compare the biological properties of six calcium silicate cements in human osteoblastic cell culture (Saos-2 cells): Bio-C Repair (Bio-C), PBS HP (PBS-HP), Biodentine (Biodentine), MTA Repair HP (MTA-HP), NeoMTA Plus (NeoMTA-P), and ProRoot MTA (ProRoot). After exposure to these materials, the cells were analyzed by MTT, wound healing, cell migration, and alkaline phosphatase activity (ALP) assays, real-time PCR (qPCR) analysis of the osteogenesis markers (osteocalcin or bone gamma-carboxyglutamate protein, BGLAP; alkaline phosphatase, ALPL; bone sialoprotein or secreted phosphoprotein 1, BNSP), and alizarin red staining (ARS). Curiously, the migration rates were low 24–48 h after exposure to the materials, despite the cells showing ideal rates of viability. The advanced and intermediate cell differentiation markers BGLAP and BNSP were overexpressed in the Bio-C, MTA-HP, and ProRoot groups. Only the Biodentine group showed ALPL overexpression, a marker of initial differentiation. However, the enzymatic activity was high in all groups except Biodentine. The mineralization area was significantly large in the NeoMTA-P, ProRoot, PBS-HP, MTA-HP, and Bio-C groups. The results showed that cellular environmental stiffness, which impairs cell mobility and diverse patterns of osteogenesis marker expression, is a consequence of cement exposure. Environmental stiffness indicates chemical and physical stimuli in the microenvironment; for instance, the release of cement compounds contributes to calcium phosphate matrix formation with diverse stiffnesses, which could be essential or detrimental for the migration and differentiation of osteoblastic cells. Cells exposed to Bio-C, PBS-HP, ProRoot, NeoMTA-P, and MTA-HP seemed to enter the advanced or intermediate differentiation phases early, which is indicative of the diverse potential of cements to induce osteogenesis. Cements that quickly stimulate osteoblast differentiation may be ideal for reparative and regenerative purposes since they promptly lead to dentin or bone deposition.
Optimizing cerebral perfusion and hemodynamics during cardiopulmonary bypass through cannula design combining in silico, in vitro and in vivo input
Authors: Hugenroth Kristin , Borchardt Ralf , Ritter Philine , Groß-Hardt Sascha , Meyns Bart , Verbelen Tom , Steinseifer Ulrich , Kaufmann Tim A. S. , Engelmann Ulrich M. 
Published: August 2021

AbstractCardiopulmonary bypass (CPB) is a standard technique for cardiac surgery, but comes with the risk of severe neurological complications (e.g. stroke) caused by embolisms and/or reduced cerebral perfusion. We report on an aortic cannula prototype design (optiCAN) with helical outflow and jet-splitting dispersion tip that could reduce the risk of embolic events and restores cerebral perfusion to 97.5% of physiological flow during CPB in vivo, whereas a commercial curved-tip cannula yields 74.6%. In further in vitro comparison, pressure loss and hemolysis parameters of optiCAN remain unaffected. Results are reproducibly confirmed in silico for an exemplary human aortic anatomy via computational fluid dynamics (CFD) simulations. Based on CFD simulations, we firstly show that optiCAN design improves aortic root washout, which reduces the risk of thromboembolism. Secondly, we identify regions of the aortic intima with increased risk of plaque release by correlating areas of enhanced plaque growth and high wall shear stresses (WSS). From this we propose another easy-to-manufacture cannula design (opti2CAN) that decreases areas burdened by high WSS, while preserving physiological cerebral flow and favorable hemodynamics. With this novel cannula design, we propose a cannulation option to reduce neurological complications and the prevalence of stroke in high-risk patients after CPB.
Recovery of phosphorus as soluble phosphates from aqueous solutions using chitosan hydrogel sorbents
Authors: Jóźwiak Tomasz , Kowalkowska Agata , Filipkowska Urszula , Struk-Sokołowska Joanna , Bolozan Ludmila , Gache Luminita , Ilie Marius 
Published: August 2021

AbstractThis manuscript presents new method of phosphorus recovery from aqueous solutions in a convenient form of readily-soluble phosphates using chitosan hydrogels. Non-modified chitosan hydrogel granules (CHs) and chitosan hydrogel granules crosslinked with epichlorohydrin (CHs-ECH) served as orthophosphate ion carriers. The developed method was based on cyclic sorption/desorption of orthophosphates, with desorption performed in each cycle to the same solution (the concentrate). The concentrations of orthophosphates obtained in the concentrates depended on, i.a., sorbent type, sorption pH, source solution concentration, and desorption pH. Phosphorus concentrations in the concentrates were even 30 times higher than these in the source solutions. The maximum concentrate concentrations reached 332.0 mg P-PO4/L for CHs and 971.6 mg P-PO4/L for CHs-ECH. The experimental series with CHs-ECH were characterized by higher concentrations of the obtained concentrate, however the concentrates were also more contaminated with Cl− and Na+ ions compared to series with CHs. The high content of chlorine and sodium ions in the concentrates was also favored by the low pH of sorption (pH < 4) and very high pH of desorption (pH > 12) in the cycles. After concentrate evaporation, phosphorus content in the sediment ranged from 17.81 to 19.83% for CHs and from 16.04 to 17.74% for CHs-ECH.
Jellyfish skin polysaccharides enhance intestinal barrier function and modulate the gut microbiota in mice with DSS-induced colitis
Authors: Cao Yu , Gao Jingzhu , Zhang Lihua , Qin Ningbo , Zhu Beiwei , Xia Xiaodong 
Published: August 2021

Jellyfish skin polysaccharides, isolated from Rhopilema esculentum Kishinouye, enhance intestinal barrier function and modulate the gut microbiota in mice with DSS-induced colitis.
A high-performance short-wave infrared phototransistor based on a 2D tellurium/MoS2 van der Waals heterojunction
Authors: Yao Jinrong , Chen Fangfang , Li Juanjuan , Du Junli , Wu Di , Tian Yongtao , Zhang Cheng , Yang Jinke , Li Xinjian , Lin Pei 
Published: August 2021

A gate-tunable Te/MoS2 vdW heterostructure is fabricated, exhibiting favourable photodetection properties with a response spectrum covering the whole SWIR band.
Network-based approach to identify prognosis-related genes in tamoxifen-treated patients with estrogen receptor-positive breast cancer
Authors: Wang Yanyan , Gong Xiaonan , Zhang Yujie 
Published: August 2021

Abstract
Tamoxifen is an estrogen receptor (ER) antagonist that is most commonly used for the treatment of ER-positive breast cancer. However, tamoxifen resistance remains a major cause of cancer recurrence and progression. Here, we aimed to identify hub genes implicated in the progression and prognosis of ER-positive breast cancer following tamoxifen treatment. Microarray data (GSE9893) for 155 tamoxifen-treated primary ER-positive breast cancer samples were obtained from the Gene Expression Omnibus database. In total, 1706 differentially expressed genes (DEGs), including 859 up-regulated and 847 down-regulated genes, were identified between relapse and relapse-free samples. Weighted correlation network analysis clustered genes into 13 modules, among which the tan and blue modules were the most significantly related to prognosis. From these two modules, we further identified and validated two prognosis-related hub genes (G-rich RNA sequence binding factor 1 (GRSF1) and microtubule-associated protein τ (MAPT)) via survival analysis based on several publicly available datasets. High expression of GRSF1 predicted poor prognosis, whereas MAPT indicated favorable outcomes in ER-positive breast cancer. Using breast cancer cell lines and tissue samples, we confirmed that GRSF1 was significantly up-regulated and MAPT was down-regulated in the tamoxifen-resistant group compared with the tamoxifen-sensitive group. The prognostic value of GRSF1 and MAPT was also verified in 48 tamoxifen-treated ER-positive breast cancer patients in our hospital. Gene set enrichment analysis (GSEA) suggested that GRSF1 was potentially involved in RNA degradation and cell cycle pathways, while MAPT was strongly linked to immune-related signaling pathways. Taken together, our findings established novel prognostic biomarkers to predict tamoxifen sensitivity, which may facilitate individualized management of breast cancer.
Adaptive event‐triggered control of multi‐agent systems with state constraints and unknown disturbances
Authors: Zhang Xi‐Zi , Lan Jie , Liu Yan‐Jun , Liu Lei 
Published: August 2021

AbstractThe leader‐following consensus problem of a class of non‐strict feedback multi‐agent systems with unknown disturbances, this paper develops a novel adaptive event‐triggered control strategy ground on the consideration of the full state constraints. Combining the dynamic surface control and event triggering mechanism, the unknown external disturbances are estimated by designing a disturbance observer. In order to deal with unknown functions, this paper employs the neural networks. On purpose of keeping the output variable within a constraint boundary, the barrier Lyapunov function is chosen during the design course. Then, through the backstepping method and the Lyapunov stability theorem, it is proved that all signals in the closed‐loop systems are bounded, and the control performance of the closed‐loop system is ensured by selecting design parameters reasonably. Eventually, the availability of the control strategy is guaranteed through experimental example.
Exploring longitudinal driving behaviour on a freeway deceleration lane using field operational test data
Authors: Lyu Nengchao , Wang Yugang , Wu Chaozhong , Wu Haoran , Wen Jiaqiang 
Published: August 2021

AbstractThe deceleration lane isbefore numbers to minus signs. a critical part of the freeway, enabling vehicles to exit the expressway safely and in an orderly fashion. However, drivers are human and thus make subjective decisions while driving; as such, each driver may approach and traverse the deceleration lane differently. This variance, which can cause major traffic disruptions and collisions, can be observed – and even mitigated, as proposed herein – based on a driver's particular characteristics. To study the variances in the longitudinal vehicle positions and microscopic operating characteristics of different drivers on a freeway exit area, a field operational test involving 46 subjects was carried out to collect data on driver characteristics, vehicle motion postures, micro‐driving operations, and road geometric elements. The 46 participants were observed based on experience and gender, and the mathematical statistical method was used to analyse driving differences in the deceleration lane. The results show that (1) the vehicle motion state can be divided into four operational stages on the deceleration lane: the pre‐deceleration process, the dynamic adjustment process, the first braking process, and the second braking process; (2) drivers generally adopted deceleration behaviour rather than maintain uniform speed when driving in the taper; (3) about 50% of drivers braked after entering the deceleration lane; (4) male drivers and skilled drivers were more inclined to drive at a higher speed on the deceleration lane, and female drivers showed a sharp increase in braking frequency once they travelled 110–150 m downstream of the taper starting point. The results of this study provide data and insights for deceleration lane design, traffic management, and driver training.
New analysis on two‐variable converter impedance model in grid‐connected systems
Authors: Shi Ying , Sun Yue , Liu Junliang , Du Xiong , Tai Heng‐Ming 
Published: August 2021

AbstractThis paper presents an in‐depth analysis for the two‐variable impedance model of a grid‐connected system. The two‐variable model describes the impedance using the frequency as well as the operating point variable. The impedance‐based stability analysis method cannot be directly applied because the conventional models are derived for a specific steady‐state operating point and the operating point varies during operation. Thus, this paper develops a new analysis method to shed light on the role the two‐variable model plays instability analysis. The proposed method not only analyzes the system stability at different operating points, but also enables the controller design to ensure system stability. Experimental results are presented to demonstrate the effectiveness of the proposed method.
Editorial
Authors: Reimer Jens 
Published: August 2021

Liebe Leserin, lieber Leser,ich weiß nicht, ob Sie ‚White Lines‘ - eine in der Hauptsache
auf Ibiza spielende Netflix Serie aus dem Frühjahr 2020 - gesehen haben. Die
Süddeutsche Zeitung schrieb „eine Krimi-, Familien- und
Hedonismusgeschichte mit skurrilen Elementen, elektronischer Musik und
spektakulär inszenierten Partys“. Die Serie, die ich vor gut einem
Jahr vergnügt verfolgt habe, fiel mir beim Lesen des Beitrags der Autoren
Köhler und Grau, Köln, zum Thema ‚Kokain abseits von
pathologischem Konsum‘ ein.
Low Flow Venoarterial ECMO Support Management in Postcardiac Surgery Patient
Authors: Airan Ritu , Sharan Sandeep , Kapoor Poonam Malhotra , Chowdhury Ujjwal , Devagourou V. , John Archip 
Published: August 2021

AbstractVenoarterial extracorporeal membrane oxygenation (VA ECMO) aims to fully support heart and lung function in postcardiac patients, ensuring end-organ perfusion and allowing time for possible heart recovery. VA ECMO is considered the modified and extended form of cardiopulmonary bypass (CPB), which is used to provide adequate tissue oxygenation. ECMO and the associated management protocols will mechanically support the patient and allow for the optimization of all aspects of care for the period of time necessary for the recovery of native cardiac or respiratory function. Significant technical advancements have been made in the equipment and the clinical management available for short- and long-term ECMO application. There are various parameters that have to be considered to evaluate whether the level of perfusion in patients on VA ECMO is adequate, and the outcomes of patients depend on them.
An ultralow-noise superconducting radio-frequency ion trap for frequency metrology with highly charged ions
Authors: Stark J. , Warnecke C. , Bogen S. , Chen S. , Dijck E. A. , Kühn S. , Rosner M. K. , Graf A. , Nauta J. , Oelmann J.-H. , Schmöger L. , Schwarz M. , Liebert D. , Spieß L. J. , King S. A. , Leopold T. , Micke P. , Schmidt P. O. , Pfeifer T. , Crespo López-Urrutia J. R. 
Published: August 2021

We present a novel ultrastable superconducting radio-frequency (RF) ion trap realized as a combination of an RF cavity and a linear Paul trap. Its RF quadrupole mode at 34.52 MHz reaches a quality factor of Q ≈ 2.3 × 105 at a temperature of 4.1 K and is used to radially confine ions in an ultralow-noise pseudopotential. This concept is expected to strongly suppress motional heating rates and related frequency shifts that limit the ultimate accuracy achieved in advanced ion traps for frequency metrology. Running with its low-vibration cryogenic cooling system, electron-beam ion trap, and deceleration beamline supplying highly charged ions (HCIs), the superconducting trap offers ideal conditions for optical frequency metrology with ionic species. We report its proof-of-principle operation as a quadrupole-mass filter with HCIs and trapping of Doppler-cooled 9Be+ Coulomb crystals.
Single-cycle all-fiber frequency comb
Authors: Xing Sida , Lesko Daniel M. B. , Umeki Takeshi , Lind Alexander J. , Hoghooghi Nazanin , Wu Tsung-Han , Diddams Scott A. 
Published: August 2021

Single-cycle pulses with a deterministic carrier-envelope phase enable the study and control of light–matter interactions at the sub-cycle timescale, as well as the efficient generation of low-noise multi-octave frequency combs. However, current single-cycle light sources are difficult to implement and operate, hindering their application and accessibility in a wider range of research. In this paper, we present a single-cycle 100 MHz frequency comb in a compact, turnkey, and reliable all-silica-fiber format. This is achieved by amplifying 2 µm seed pulses in heavily doped Tm:fiber, followed by cascaded self-compression to yield 6.8 fs pulses with 215 kW peak power and 374 mW average power. The corresponding spectrum covers more than two octaves, from below 700 up to 3500 nm. Driven by this single-cycle pump, supercontinuum with 180 mW of integrated power and a smooth spectral amplitude between 2100 and 2700 nm is generated directly in silica fibers. To broaden applications, few-cycle pulses extending from 6 µm to beyond 22 µm with a long-term stable carrier-envelope phase are created using the intra-pulse difference frequency, and electro-optic sampling yields comb-tooth-resolved spectra. Our work demonstrates the first all-fiber configuration that generates single-cycle pulses and provides a practical source to study nonlinear optics on the same timescale.
The doping engineering and crystal structure of rod-like Au8Ag17 nanoclusters
Authors: Lin Xinzhang , Tang Jie , Zhang Jubo , Yang Yang , Ren Xiuqing , Liu Chao , Huang Jiahui 
Published: August 2021

Alloy nanoclusters protected by ligands were widely studied due to the synergistic effect of metal atoms, and they exhibit enhanced properties in different fields, such as bio-imaging and catalysis. Herein, we obtained Au8Ag17(PPh3)10Cl10 nanoclusters via one-step simple synthesis. The atomically precise crystal structure was determined by x-ray crystallography. It is found that the rod-like Au8Ag17 nanoclusters were composed of two Au4Ag9 icosahedrons via sharing the same Ag atom. Two Au atoms occupy the center of icosahedrons, and the other six Au atoms are all at the neck sites. Four kinds of Cl–Ag connecting modes were observed in Au8Ag17 nanoclusters. Moreover, the ultraviolet–visible absorption spectrum shows that the prominent absorption peaks of Au8Ag17 nanoclusters are at ∼395 and 483 nm. This work provides a feasible strategy to synthesize alloy nanoclusters with precise composition via doping engineering.