bluuebunny/crossref_metadata_2025_split huggingface dataset:
A novel cell type negatively associated with secondary autoimmunity in alemtuzumab-treated patients is revealed through single-cell longitudinal analysis of clinical trial samples
Authors: Savova Virginia , Hanamsagar Richa , Chamberlain Mathew , Shankara Srinivas , Marcus Robert , Baker Darren , Klinger Katherine , Havari Evis , Nestle Frank , de Rinaldis Emanuele 
Published: June 2021

Abstract
Alemtuzumab, a humanized anti-CD52 monoclonal antibody, is an approved treatment for relapsing forms of multiple sclerosis (RMS). While its efficacy has been demonstrated in clinical studies, its use is associated with unpredictable non-MS autoimmunity manifesting months or years after treatment. Approximately 40% of treated patients present with autoimmune thyroid events, 2% with platelet deficiency (immune thrombocytopenia; ITP)1, and 0.34% with autoimmune nephropathies2. The lack of predictive biomarkers necessitates careful monitoring in clinical practice with a Risk Management Plan or Risk Evaluation and Mitigation Strategy (RMP/REMS) in place for early detection of these autoimmune events. We carried out a longitudinal single-cell analysis of PBMCs in a small subset of alemtuzumab-treated patients from the phase 3 CARE-MS I (CAMMS323) study3 and identified a novel platelet lineage cell negatively associated with thyroid autoimmunity. This discovery raises the possibility that a shared underlying mechanism may contribute to the incidence of thyroid autoimmunity and ITP in alemtuzumab-treated patients.
Multivariate Analysis to Assess the Quality of Irrigation Water in a Semi-Arid Region of North West of Algeria : Case of Ghrib Dam
Authors: HALLOUZ FAIZA , Meddi Mohamed , Rahmani Salaheddine Ali 
Published: June 2021

Abstract
Dams are critical to agriculture, industry, and the needs of humans and wildlife. This study evaluates the water quality of the Ghrib dam in north west of Algeria, using Irrigation Water Quality Index (IWQI), sodium absorption rates (SAR) and multivariate statistical methods (Clustering and principal component analysis). The study concerns the analysis of physical and chemical parameters (pH, EC, O2, TUR, Ca, Mg, HCO3, Na, K, BOD, DCO, Cl−, PO4, SO3. NH4 et NO3) which were measured at twelve selected points along the dam over 8 periods (dry and wet periods) using standard methods. Irrigation Water Quality Index values in the dam were found to be between 41 and 59, according to classifications for different water uses, values below 60 indicate that water is of poor quality for irrigation and treatment is recommended to make dam water more suitable for irrigation. The results of water analysis in our study area reveal the presence of acute pollution which is certainly caused by direct releases of either industrial or domestic origin, and we note that this pollution remains variable depending on the collection periods. Also, Chloride-calcium and sulfate facies are the most dominant in sampling periods for dam water, resulting in poor water quality for irrigation. In addition, water is, therefore, highly mineralized and is likely to be suitable for irrigation of certain species (cucumbers...) that are well tolerant to salt and on well-drained and leached soils.
Does higher health literacy lead to higher trust in public hospitals?
Authors: Bertram Maja , Brandt Urs Steiner , Hansen Rikke Klitten , Svendsen Gert Tinggaard 
Published: June 2021

Abstract
Background:

Does higher health literacy lead to higher trust in public hospitals? Existing literature suggests that this is the case since a positive association between the level of health literacy and the level of trust in physicians and the health care system has been shown. This study aims to challenge this assumption.
Methods:

Based on theoretical arguments from game theory and analysis of empirical data, we argue that the association is better described as an inversely u-shaped curve, suggesting that low and high levels of health literacy lead to a lower level of trust than a medium level of health literacy does. The empirical analysis is based on a study of the Danes’ relationship to the overall health care system. More than 6,000 Danes have been asked about their overall expectations of the health service, their concrete experiences and their attitudes to a number of change initiatives.
Results:

Game theory analysis show that the combined perceived cooperation and benefit effects can explain an inversely u-shaped relationship between social groups and trust in the health care system. Based on quantitative, binary regression analyses of empirical data, the lowest degree of trust is found among patients from the lowest and highest social groups, while the highest degree of trust is found in the middle group. The main driver for this result is that while patients having low health literacy perceive that the health care system is not cooperative, patients with a high level of health literacy have high expectations about the quality, which the health care system might not be able to provide. This reduces the perceived benefit from their encounter with the health care system.
Conclusion:

It is important that health care professionals understand that some patient groups have a higher chance of cooperation (e.g., agreeing on the choice of treatment) or defection (e.g. passing a complaint) than others. In perspective, future research should undertake further qualitative examinations of possible patient types and their demands in relation to different health care sectors, focusing specifically on the opportunities to improve the handling of different patient types.
Laser-triggered combined chemotherapy and photodynamic therapy enabled by iron sulfide-doxorubicin@bovine lactoferrin nanozymes for breast cancer therapy
Authors: Ning Shipeng , Zheng Yang , Qiao Kun , Li Guozheng , Xu Shouping , Bai Qian 
Published: June 2021

Abstract
BackgroundThe use of magnetic nanozymes with the ability to synchronize gas therapy through photodynamic and chemotherapy in the treatment of breast cancer has received much attention. ResultsHence, in this study, we designed a bovine lactoferrin-coated iron sulfide nanozymes containing doxorubicin (FeS-Dox@bLf NZs) by wet chemical synthesis that can respond to tumor acidity. Then, the physicochemical properties of synthesized NZs were investigated by TEM, SEM, DLS and spectroscopic methods. Likewise, the level of Fe2+ release, H2S and Dox from FeS-Dox@Lf NZs under acidic conditions was evaluated. It was observed that Fe2+ and S2- caused significant OH and H2S release through the Fenton reaction. Also, the toxic effects of FeS-Dox@Lf NZs on 4T1 cancerous cells were investigated by MTT and flow cytometry assays. After intravenous injections of NZs and laser irradiation, significant effects of FeS-Dox@Lf NZs on mice weight and tumor status were observed. Afterwards, not only the distribution of Dox in the body was examined by fluorescent, but also the time of Fe clearance and the amount of Dox and Fe retention in vital tissues were determined. The findings confirm that FeS-Dox@Lf NZs, in addition to targeted drug distribution in tumor tissue, resulted in superior therapeutic performance compared to free Dox due to reduced Dox side effects in vital tissues, and increased level of free radicals in 4T1 cells. ConclusionOverall, FeS-Dox@Lf nanozymes with the ability to synchronize chemotherapy and gas therapy raised hopes for more effective treatment of breast cancer.
Spatial-Temporal Heterogeneity of Rock Samples’ Deformation and Damage: Experimental Study and Digital Image Correlation Analysis
Authors: Yan Fayuan , Qi Chengzhi , Shan Renliang 
Published: June 2021

Abstract
In-situ observations and laboratory experiments showed that slow deformation waves widely exist in geomedia under loading conditions. Slow deformation waves’ behavior exhibits some similarities in media ranging from the scale as large as the Earth's crust to the scale as small as the laboratory test samples. However, the mechanism underlying their generation has not been clarified yet. In this research an experimental study was performed on small-scale red sandstone samples subjected to uniaxial compression at the displacement rates of 0.1, 0.5, and 1 mm/min. Slow deformation waves under different loading rates were analyzed by speckle photography for microscopic characterization combined with the digital image correlation (DIC) technique. The Luders deformation bands were predominantly observed in the flow channels formed at the stage of macro-elastic deformation. The spatial-temporal heterogeneity of the rock sample surface was quantified, and the deformation waves' propagation velocities under different loading rates were obtained. The linear relationship between the propagation velocities of slow deformation waves and the loading rates was determined. The research findings shed some new lights on the evolutionary characteristics of the slow deformation waves.
On the Quest of Small Molecules that Can Mimic Psalmotoxin-1, a Powerful Peptidic Modulator of the Acid Sensing Channel ASIC1a
Authors: Pietra Francesco 
Published: June 2021

Abstract
Acid-sensing ion channels (ASICs) are thought to play a key role in a number of pathologies, from neuronal injury to pain sensation, while no drug has yet been approved as their modulator. This work was devised to asses roughly, yet from first principles, the relative energies of binding in the most important acidic pocket of cASIC1a, thereby paving the way to find small molecules that can mimic Pctx1, the most powerful peptidic modulator of cASIC1a. To this end, MD simulations for the overall conformation, and QM-MM simulations specifically for the location of hydrogen atoms, allowed disentangling the relative weight of the various non-bonded interactions between PcTx1 and cASIC1a. Main weight could be attributed to deeply buried salt bridges formed by the guanidinium end chains of residues Arg26 and Arg27 on PcTx1 and carboxylate end chain of distant residues Asp and Glu on cASIC1a. Rewardingly, in a preliminary attempt at exploiting these observations toward a small-molecule modulator, a Arg26-Arg27 stretch, excised from the PcTx1-cASIC1a complex and slightly simplified, on automated rigid docking on ligand-free receptor was observed to form most of the above salt bridges.
Implementation and Evaluation of Urban Green Space Supply for Debre Berhan Town, Ethiopia
Authors: Dejen Awlachew Dejen , Yitagesu Ayele Behaylu , Aysassa Dodge Getachew 
Published: June 2021

Abstract
Urban green space (UGS) is part of urban land covered with vegetation which has an immense benefit for sustainable urban development. The main objective of this study was urban green space supply analysis and evaluation for Debre Berhan town, Ethiopia. To realize this objective, we generated UGSs information by using normalized difference vegetation index (NDVI) which was calculated from Sentinel 2 satellite image. Then, the result of NDVI calculation was classified as small vegetation and high vegetation with over all accuracy of 87.29% and kappa value of 0.808. According to the result, there are 15.85% and 1.55% supply of urban green space for small and high vegetation respectively. In addition, the UGS supply in inner and outer part of the town for each land use was quantified. The result showed that in the inner part of the town, service, green area, and vacant land uses have 30.12%, 30.06%, and 13.32% of UGS supply respectively. Conversely, recreation, commercial, and administration land uses are least supplied with 0.11%, 0.26%, and 0.40% share of UGS. Furthermore, the UGS supply was evaluated against the new urban planning strategy of Ethiopia (30% coverage) and World Health Organization standard (9m2 per person). The coverage of small vegetation and high vegetation was 15.85% and 1.55% respectively. The UGS per capita for small vegetation was about 75.16m2 while the value for high vegetation was 7.33m2. The results of our analysis can be used as an input for urban planning and master plan revision.
Comparative Proteomic Analysis of Erythropoiesis Tissue Head Kidney Among three Antarctic Fish Species
Authors: Jia Ruonan , Huang Shaojun , Zhai Wanying , Jiang Shouwen , Li Wenhao , Wang Faxiang , Xu Qianghua 
Published: June 2021

Abstract
Antarctic icefish is the only known vertebrate species that lacks oxygen-carrying hemoglobin and functional erythrocytes. To reveal the unique hematopoietic process of icefish, we used an integrated approach including tandem mass tag (TMT) labeling and liquid chromatography-tandem mass spectrometry (LC-MS/MS) to quantify the dynamic changes in the head kidney whole proteome of a white-blooded icefish, Chionodraco hamatus, compared to those in two other red-blooded Antarctic fish, Trematomus bernacchii and Notothenia coriiceps. Of the 4,672 identified proteins, in the Antarctic ice fish head kidney, 123 proteins were significantly up-regulated and 95 proteins were down-regulated. The functional grouping of differentially expressed proteins based on KEGG pathway analysis shows that white blood fish and red blood fish have significant differences in erythropoiesis, heme biogenesis, leucocyte and platelet cell development. The proteins involved in the hematopoietic process in icefish showed a clear trend of downregulation of erythroid lineage marker proteins and upregulation of lymphoid and megakaryocytic lineage marker proteins, including CD9, ITGB2, and MTOR, which suggests a shift in hematopoiesis in the icefish head kidney due to the loss of erythrocytes. The results of the present study not only provide basic datasets for the head kidney proteins of Antarctic fishes, but also provide important references for studies on immunity and hematopoiesis in various species.
Psychiatric complications (depression, anxiety disorders, post-traumatic stress disorder) of ARDS: a systematic review
Authors: Klimcik Nela , Nguyen Maxime , D'Athis Philippe , Forestier Nathalie , Guinot Pierre-grégoire , Gelinier Jean Christophe Chauvet , Bouhemad Belaid 
Published: June 2021

Abstract
Background: ARSDS requires extensive resuscitation and has major physical sequelae. We evaluated the psychiatric sequelae by conducting a systematic review to determine prevalence of symptoms of depression, anxiety disorders and post-traumatic stress disorder (PTSD) following ARDS, as well as their risk factors.Methods: We conducted a search on Pubmed by including all studies meeting our primary endpoint (prevalence of symptoms of depression, anxiety disorders and PTSD following ARDS) and secondary endpoint (associated risk factors). Results: Included studies found prevalence ranging from 16 to 46% for symptoms of depression, 23 to 62% for symptoms of anxiety disorders and 16 to 47% for symptoms of PTSD. The data were too heterogeneous to conduct a meta-analysis. Among the risk factors associated with a risk factor of depression, anxiety disorders or PTSD after ARDS were; factors related to patient (as an history of mental illness) risk factors related to ICU management (as use opioids and benzodiazepine). Effect of severity of ARDS is not known.Conclusion: This systematic review shows that depression, anxiety disorders and PTSD following ARDS are frequent, long-lasting, disabling and have severe psychiatric consequences. Associated risk factors can be identified and among us use of opioids.
The Views of Public Service Managers on the Implementation of National Health Insurance in Primary Care
Authors: Murphy Shane Darren , Moosa Shabir Ahmed 
Published: June 2021

Abstract
Background The South African government is implementing National Health Insurance as a monopsony health care financing mechanism to drive the country towards Universal Health Coverage. Strategic purchasing, with separation of funder, purchaser and provider, underpins this initiative. The NHI plans contracting units for primary healthcare services to function as independent sub-district purchasers and District Health Management Offices to support and monitor these contracting units. This decentralised governance model to the operational unit of primary healthcare, the heartbeat of any universal healthcare system, is critical to programme success. The views of district-level managers, who are at the centre of the planned phased rollout will shed light on current policy implementation.ObjectivesThis is a qualitative study to explore district and sub-district managerial views on National Health Insurance and its implementation. Methods Purposive sampling was used to identify key respondents from a major urban district in Gauteng, South Africa, for participation in exploratory in-depth interviews. This study employed framework analysis within MaxQDA software for robust thematic analysis. Results Managers viewed National Health Insurance as a social and moral imperative but lacked clarity and insight into the National Health Insurance Bill and relevant implementation strategies. The majority of respondents had not received any engagement or had the opportunity to engage in policy formulation. District managers highlighted several pitfalls in current organisational operations. National and provincial government continue to function in a detached and rigid top-down hierarchy. The voices of coalface managers and workers, who live the reality of South African healthcare service provision, go unheard and unengaged. The findings of this study dishearteningly echo lessons already learned around established pillars of universal healthcare implementation such as human resources, multi-lateral stakeholder engagement and collaboration, devolution of governance with empowerment and capacitation of district managers. These findings imply that the South African Government has failed to anticipate and address these challenges and raises questions around reflective and experiential practices of the South African government. Conclusion It appears that strategic purchasing is not being operationalised in PHC. NHI policy implementation appears trapped in a rigid top-down hierarchy. District managers need to be engaged and capacitated to operationalise the planned decentralised purchasing-provision function of NHI.
An Effective Hybrid and Adaptive Caching Network Framework Approach In Time-to–Live (TTL) Based Data Item for Peripatetic Computing Environment
Authors: P Srimanchari , G Anandharaj 
Published: June 2021

Abstract
Caching is a well established technique to improve the efficiency of data access. This research paper introduces a Hybrid and Adaptive Caching (HAC) approach to cache the data item based on the varying size, and, Time-to-Live (TTL) based invalidation of the data item in a mobile computing environment. Mobile nodes establish single-hop communication with the base station and ad-hoc peer to peer communication with other neighbor nodes in the network to access data items. The proposed work adjusts the caching functionality level based on the size of the data item and stores the cached data item in two different storage systems. The cache of each node is separated into Temporary Buffer (TB) and Permanent Buffer (PB) to improve the data access efficiency. This approach is based on the fact that the smaller size data (e.g. stocks) are updated for shorter Time-to-Live (TTL) whereas the larger size data (e.g. video) are updated only for longer TTL. This proposed work also suggests an adaptive cache replacement and cache invalidation technique to resolve the issues regarding bandwidth utilization and data availability. In cache replacement technique, the cached data item is effectively replaced based on the size of the data item and TTL value. A timestamp-based cache invalidation strategy where the cached data is validated according to the update history of the data items has also been introduced in this paper. The threshold values have greater impact on the system performance. Therefore, the threshold values are fine tuned such that they do not affect the system performance. The proposed approaches significantly improve the query latency, cache hit ratio and efficiently utilize the broadcast bandwidth. The simulation result proves that the proposed work outperforms the existing caching techniques.
Reproducibility of Choroidal Thickness Measurements in Hemodialysis Patients. A Spectral Domain Optical Coherence Tomography Study
Authors: Eroğul Özgür , Uzel Mehmet Murat , Eroğul Leyla Eryiğit , Uzel Ayşe Güzin Taşlıpınar , Gobeka Hamidu Hamısı , İbiş Avşin 
Published: June 2021

Abstract
PurposeTo investigate the effects of hemodialysis (HD) on the reproducibility of subfoveal choroidal thickness (SFCT) as measured by spectral domain-optical coherence tomography (SD-OCT)MethodsIn this study, 26 HD (26 eyes) patients had their pre- and post-HD SFCT measured, and the results were compared for reproducibility. Following a thorough ophthalmic examination, SD-OCT was performed three times in a row during a single session. The same physician measured SFCT after automatically identifying choroid with a software caliper. The reproducibility parameters, including intra-class correlation coefficients (ICCs), coefficients of variation (COV), and test-retest variability (TRTV) were then calculated.ResultsMales made up 53.85% of the 26 HD patients. There was a significant IOP difference between pre-HD (16.42±3.14 mmHg) and post-HD (14.21±2.78 mmHg) (P<0.001). SFCT decreased significantly from pre-HD 243.50±10.23 μm to post-HD 234.29±9.41 μm (P<0.001). ICC value increased significantly after HD, rising from 0.948 to 0.989 (P<0.001, for all). Pre- and post-HD COV values were 1.6% and 0.65%, respectively. Also, pre- and post-HD TRTV values were 7.864±1.996 μm and 3.074±1.536 μm, respectively.ConclusionThe reproducibility of SFCT as measured by OCT was lower during pre-HD compared to post-HD. Post-HD SD-OCT assessment appears to improve the reliability of clinical outcomes in the diagnosis and monitoring of HD patients.