bluuebunny/crossref_metadata_2025_split huggingface dataset:
Aspectos clave para la transformación genética de arroz (Oryza sativa L.) subespecie indica mediante Agrobacterium tumefaciens
Authors: Aguilar-Bartels Cindy Marié , Quirós-Segura Priscillla , García-Piñeres Alfonso , Gatica-Arias Andrés , Arrieta-Espinoza Griselda 
Published: September 2021

Introducción. La transformación del arroz (Oryza sativa L. ssp indica) mediada por Agrobacterium, representa una oportunidad para la investigación científica y el mejoramiento genético. Es necesaria la optimización del protocolo para obtener la mayor eficiencia de transformación. Objetivo. Evaluar diferentes factores que afectan la transformación genética en callos embriogénicos de arroz de la subespecie indica vía Agrobacterium tumefaciens. Materiales y métodos. Este estudio se realizó en San José, Costa Rica, entre 2012 y 2014. En seis tratamientos se evaluaron: el efecto de la edad del callo, la concentración de acetosiringona, condición luminosa, la presencia o ausencia de radícula y la cepa de Agrobacterium tumefaciens en la transformación genética de callos embriogénicos de arroz de la variedad CR5272 con el gen reportero gus. Se compararon la cepa de Agrobacterium LBA4404 con el plásmido pCAMBIA1305.2 y las cepas ATHV, GV3101 y LBA4404, con el plásmido pCAMBIA1303; mediante pruebas histoquímicas para la detección de la expresión transitoria del gen marcador que codifica para la β-glucuronidasa. Resultados. La evaluación de los seis tratamientos con la cepa LBA4404::pCAMBIA1305.2 resultó en expresión transitoria del gen GusPlus de 1,33-7,00 % para la variedad CR5272 y de 8,00 % para el control con la variedad Nipponbare (ssp. japonica). Las cepas con el plásmido pCAMBIA1303 presentaron una expresión transitoria del gen gusA entre el 100-65 % con un área promedio de 14,23 mm2 (ATHV), 8,81 mm2 (GV3101), y 8,83 mm2 (LBA4404), sin diferencias significativas entre ellas; sin embargo, sí hubo diferencias al compararlas con la cepa LBA4404::pCAMBIA1305.2 (85 %, 4,39 mm2). Conclusiones. La utilización de las condiciones: callos de seis días, concentración de acetosiringona de 76 µM, luz antes y después del cocultivo, presencia de radícula y la cepa ATHV::pCAMBIA 1303, mejoraron la eficiencia de transformación con Agrobacterium tumefaciens en la variedad de arroz CR5272.
The mother of yaly to the study of the operation with local anesthesia according to Schleich'y
Authors: Neschel V. E. 
Published: September 2021

Abstracts. Surgery.
V. E. Neshel. The mother of the yaly to the study of the operation with local anesthesia according to Schleich'y. Doctor 1900, No. 49.Pointing to a sharp drop in the number of chloroformations in foreign clinics with the introduction of the Schlejch method and considering this method a true blessing for zemstvo doctors working without assistants, the author, based on his 4 cases of stone disease, in which he was the first anesthesia according to Schleich'y with a cost of 7, 5 - іо c.s. liquid No. 1, make sure that sectio alta can be produced painlessly with local anesthesia, especially where the walls of the bladder are not much altered and where it is possible to operate with a filled bladder.
About the action of tropocaine on the eye
Authors: Chirkovsky V 
Published: September 2021

Dissertation. SPb.1901.
The author made an observation of the new anestheticum proposed in the beginning of the 90s - tropocaine, which, according to Ziebermann, consisted of benzoic acid and pseudotropein; for use in medical practice, the hydrochloric acid salt of this alkaloid is recommended.
Plastic surgery of the urinary bladder from the intestine with the formation of artificial urinary tract infection in case of ectopia
Authors: Spasokukotskiy S. 
Published: September 2021

Surgery. t. VIII No. 78.
The name of the patient with ectopia of the bladder and stopping from the existing principles of treatment of this deformity on the diversion of urine into the intestine; the author did it in the following way.
To casuistic operations of exclusion of a wrist at treatment of fecal fistulas
Authors: Lisyanskiy V. I. 
Published: September 2021

Surgery vol. XIV. No. 82.
The presence of a fecal fistula is one of the indications for the operation of turning off the intestine, which is currently used in three types of enteroanastomosis and actually turning off one and two-way. The use of the exclusion operation in fecal fistulas is necessary, according to the author, in those cases, where it is impossible, in the FORCE of any conditions, to apply the most radical approach intestinal resection.
Cyclopia due to abnormality of other organs
Authors: Ognev B. V. 
Published: September 2021

Rhino-laryngo-otiatric section. Meeting on March 7, 1930
Dr. BV Ognev Cyclopia due to anomalies in other organs. See the meeting of the surgeon. sections from 8/1-30 g. The speaker was asked a number of questions - Professor V. K. Trutnev, prof. KR Viktorov and Dr. Syzganov.
Observations on athymated animals
Authors: Vorobiev V. N. 
Published: September 2021

Dr. W. Worobjew (Kasan). Beobachtungen uber athymierte Tiere.
Der Verfasser unternahm Versuche mit dem Entfernen der Kropfdriise bei 16 Ferkeln (5 Mannchen und ll Weibchen) mit 13 Kcntrolltie ren desselben Wurfes; bei einigen Ferkeln wunde die ganze Kropfdriise enfernt, bei anderennur der Halsteil; in 2 Versuchen wurde den Ferkeln mehrmalige Transplantation der Kropfdriise von thymectomierten Tieren gemacht. Die Tiere warden meist im Alter von 1 bis 3 Wochen, selten 5 Wochen genommen. Die Dauer der Beobachtungen schwankte von 73 bis 760 Tage. Diese Beobachtungen zeigten, dass das Entfernen der Kropf drilse im fruhen Alter zu einer ganzen Reihe von Veranderungen, zu allererst allgemeinen Charakters, fiihrt,Tiere mit entfernter Kropfdriise blieben in Gewicht schon von 2. Lebens:nonat an von den Kontrolltieren ischarf zuriick, die Rohrenknochen waren bei ihnen um 28 cm kiirzer, als bei den letzten, im Blute wurde der Leukocytensturz mit Pravalierung der Polynuclearen bemerkbar, die Haut verlor ihre Elastizitat, die athy mierten Tiere waren weniger lebendig, als die Kontrolltiere, mehr gefrassig, erkrankten leichter u. s. w. Von den lokalen Veranderungen verdienen besondere Aufmerksamkeit die Veranderungen in den endokrinenen Drusen: bei den athymierten Ferkeln erschien der Hypophysis 25-mal grosser, als bei den Kontrolltieren, die gl. suprarenales und gl. pinealis2-mal grosser, grosserobwohl in geringerem Masseerschien auch die gl. thy reoidea, die testes aberP/a-mal kleiner, ebenso wie die Eierstocke. Der Grad dieser Veranderungen variierte je nach dem Zeitraum, der nach dem Entfernen der Kropfdriise vergangen war.
Co-exploring the effects of COVID-19 pandemic on the livelihood of persons with disabilities in Bangladesh
Authors: Das Adity Shayontony , Bonny Fatema Akter , Mohosin Arifa Bente , Rashid Sabina Faiz , Hasan Md Tanvir 
Published: September 2021

Background: According to World Health Organization (WHO), vulnerable groups such as persons with disabilities are facing severe impacts of the pandemic. There has always been significant challenges and hurdles in terms of achieving adequate and equitable inclusivity of persons with disabilities in all sections of social life. Education and employment of persons with disabilities were least focused which created more marginalization for the community. The long term impact of these marginalization has also led to the lack of jobs and social security of persons with disabilities, which is very clear now given the crisis in place. In low and middle income countries like Bangladesh the situation is even worse. To better understand the conditions of persons with disabilities in this crisis situation, the present study was initiated to explore the dimensions of livelihood with respect to income and wellbeing of persons with disabilities and to generate evidence for developing policies around these issues.Methods: A qualitative study was undertaken among 30 persons with disabilities from 8 different geographical divisions of Bangladesh. The interviews were conducted through telephone calls due to the existing COVID-19 crisis and mobility restrictions. The respondents were purposively selected based on gender, type of disability, area of resident (urban, rural) and their ability to communicate, therefore most (25/30) respondents were persons with physical disability. Thematic analysis was conducted to generate the findings of the study.Findings: Study findings revealed that majority of the respondents were involved in informal jobs. Predominantly males were daily wage-earners and often the sole breadwinner of the families, very few females were involved in economic activities. Since they had no stable income, the economic shock from the COVID-19 pandemic had affected them badly even leading to household level famine. The study identifies low level of education and informal job security as the primary causes of socio-economic insecurity among persons with disabilities, resulting in challenges in ensuring a stable livelihood during crisis situations, such as COVID-19.Conclusion: Constant alienation of persons of disabilities from the formal sector results in the deterioration of their livelihood standards which even worsen during any emergency crisis such as COVID-19. The study pinpoints that only aided services are not adequate to ensure persons with disabilities' rights rather there is an urgent need of disability inclusion in formal job sector and livelihood training for persons with disabilities. To achieve the Sustainable Development Goals 2030 and to irradiate the inequality towards persons with disabilities in the society it is important for the Government and concern bodies to focus on the inclusiveness with better implementation and monitoring strategies.
Implementation of pharmacist-led services in primary care: A mixed-methods exploration of pharmacists’ perceptions of a national educational resource package
Authors: Preston Kate , Weir Natalie M. , Mueller Tanja , Newham Rosemary , Bennie Marion 
Published: September 2021

Background: To help alleviate the global pressure on primary care, there has been an increase in the number of clinical pharmacists within primary care. Educational resources are necessary to support this workforce and their development within this role. An educational resource package was developed in Scotland to support the General Practice Clinical Pharmacists (GPCPs), containing a hard copy Competency and Capability Framework (CCF), an online platform (TURAS) and both clinical and educational supervisors in 2016.
Objective: To examine the implementation of a competency-based educational resource package through the exploration of pharmacists’ perceptions of its adoption, acceptability, appropriateness, and feasibility.
Methods: Participants were GPCPs who had been part of a national training event between 2016 and 2018. The participants were given the opportunity to complete an online questionnaire or a semi-structured telephone interview. Both data collection tools were based on Proctor’s model of implementation outcomes: adoption, acceptability, appropriateness and feasibility. Areas covered included GPCPs’ perceptions and level of adoption of the educational resource package developed to support them in their role.
Results: Of a potential 164 participants, 52 (31.7%) completed the questionnaire and 12 (7.3%) completed the interview. GPCPs indicated widespread adoption and were accepting of the resources; however, it was suggested that its value was undermined, as it was not associated with a qualification. The appropriateness and feasibility of the resources depended on GPCPs’ individual situation (including current role, previous job experience, time available, support received from peers and supervisors, and perceptions of resources available).
Conclusions: The suitability of the CCF was evidenced by participants’ adoption and acceptance of the resource, indicating the necessity of a competence-based framework to support the GPCPs’ role. However, its suitability was hindered in terms of varied perceptions of appropriateness and feasibility. Despite the limited sample size, the results indicate that the value of these resources should be promoted across primary care; nevertheless further facilitation is required to allow GPCPs to fully engage with the resources.
Psychometric Evaluation of the School Nurses' Awareness and Perceptions Survey (SNAPS) for Youth At-Risk of Trafficking
Authors: Fraley Hannah , Aronowitz Teri 
Published: September 2021

Background and PurposeEstimates suggest approximately 244,000–360,000 schoolaged children in the United States are at risk of being trafficked. The purpose of this study was to test the psychometric properties of the School Nurses' Awareness and Perceptions Survey (SNAPS).MethodsA multi-phase approach for reliability and validity using correlation, exploratory, and confirmatory factor analysis (CFA) with samples of school nurses from Massachusetts (Phase I) and nationally (Phase II).ResultsSNAPS is a 32-item 5-point Likert scale with a potential score range of 32–160. Cronbach's alpha was .94 with sub-scales ranging from 0.84–0.94. CFA revealed a three-factor scale with a cumulative variance of 70.79% (Chisquare 3.13, df 461, p < .001; RMSEA .10; GFI .80; CFI .78; TLI .76).ConclusionsStrong psychometric properties were found for the new measure allowing assessment of school nurses' knowledge of youth trafficking.
Measuring Anticipated Loss of Aging Parents: Development of the Parent Anticipatory Loss Scale
Authors: Kartoz Connie R. , Wells Munira , Hammell Paige 
Published: September 2021

Background and PurposeResearch indicates non-caregiving adult children with aging parents experience anticipatory loss for parents that consists of feelings of gratitude, sadness, and worry. The purpose of this research was to develop the Parent Anticipatory Loss Scale.MethodsInterview data from non-caregiving adult children and extant literature formed the basis for creating items measuring three components of Parent Anticipatory Loss. Expert content validity was established prior to administering the survey to a convenience sample of non-caregiving (N = 315), mostly Caucasian (n = 182, 57.6%) men (n = 202, 63.9%). The crowdsourcing platform, Mechanical Turk, provided an innovative method for sample recruitment.ResultsQuesti ons were eliminated as indicated during analysis, yielding a 19-item scale (α = 0.93) with three subscales (α = 0.897 for Gratitude, α = 0.841 for Sadness, and α = 0.833 for Worry). Exploratory factor analysis (Varimax rotation) showed gratitude accounted for 46% of the variance, with sadness and worry accounting for 9.5% and 5.3%.ConclusionsThe Parent Anticipatory Loss scale demonstrates good initial reliability and validity and can be used to measure anticipatory loss for aging parents. Further testing with cross cultural samples is warranted.
Psychometric Analysis of the Arabic Translation of the Multidimensional Scale of Perceived Social Support
Authors: Khalil Dalia , Templin Thomas , Giurgescu Carmen , Misra Dawn P. 
Published: September 2021

BackgroundSocial support has been related to postpartum depression (PPD) among immigrant women. The Multidimensional Scale of Perceived Social Support (MSPSS) has been translated to Arabic (MSPSS-AW) but not validated among postpartum women.PurposeTo further replicate and refine the already existing MSPSS-AW to determine: (a) reliability and construct validity of the MSPSS-AW in immigrant Arab American postpartum women, and (b) relationship of dimensions of social support and PPD.MethodsUsing a cross-sectional design, 115 Arab American immigrant postpartum women completed demographic questionnaire, MSPSS-AW, and the Edinburgh Postnatal Depression Scale.ResultsConfirmatory factor analysis replicated three- factor model for social support (Family, Friends, and Husband). Internal consistency reliability coefficients for the three subscales ranged from .65 to .79. The MSPSS-AW subscales were related to PPD symptoms controlling for years of education, anxiety, and depression during pregnancy.ConclusionThe MSPSS-AW is reliable and valid for use with immigrant Arab American postpartum women.
Psychometric Evaluation of Persian Version of Hope Scale in Iranian Patients With Cancer
Authors: Nia Hamid Sharif , Rahmatpour Pardis , She Long , Abounoori Mahdi , Maddah Mohammad Moein , Sharif Saeed Pahlevan , Mohammadinezhad Mobin 
Published: September 2021

Background and PurposeAdult Hope Scale is one of the most popular tools for hope measuring. The study aimed to evaluate the psychometric properties of the Persian Version of Hope Scale among Iranian patients with cancer.MethodsIn this methodological study, patients with cancer (n= 400) were recruited by convenience sampling from June to December 2019. The construct and content validity and reliability of the scale were evaluated.ResultsA two-factor structure for the scale was indicated with the factors being optimism life, and despair that explained 60.483% of the total variance of the scale. The model has the goodness of fit and acceptable internal consistency (Cronbach’s alpha: .701–.897).ConclusionsThe Persian Version of Hope Scale demonstrated acceptable validity and reliability among patients with cancer.
Cross-Cultural Adaptation and Psychometric Evaluation of the Persian Version of the Clinical Decision-Making in Nursing Scale
Authors: Kouravand Zhaleh , Aein Fereshteh , Ebadi Abbas , Yadegarfar Ghasem 
Published: September 2021

Background and PurposeThe aim of this study was the cross-cultural adaptation and psychometric evaluation of the Persian version of Clinical Decision Making in Nursing Scale (CDMNS).MethodsThe original CDMNS was translated into Persian using the Beaton's guideline. Its qualitative face validity, qualitative and quantitative content validity, its construct validity, and reliability was assessed.ResultsEleven items out of forty items were deleted due to factor loading values less than 0.3. Subsequently, model fit indices changed as follows: Chi-square value divided by degree of freedom (𝜒2/DF): 2.8, root mean score error of approximation (RMSEA): 0.07, standardized root mean square residual (SRMR): 0.06, comparative fit index (CFI): 0.93, goodness of fit index (GFI): 0.80, and adjusted goodness of fit index (AGFI): 0.77. The Cronbach's alpha values and test.retest intraclass correlation coefficient of the 29-item scale and its subscales also increased afte deleted.ConclusionThe 29-item Persian CDMNS is a valid and reliable instrument for assessing Iranian nurses' perceptions of clinical decision-making.
Construct Validity of the Health Utilities Index Mark 2 and Mark 3 to Measure Health-Related Quality of Life of Residents in Long-Term Care Facilities
Authors: Jones C. Allyson , Paudel Yuba Raj , Slaughter Susan E. , Ickert Carla , Jhangri Gian S. , Feeny David 
Published: September 2021

Background and PurposeThe purpose is to evaluate the construct validity of two generic health measures, the Health Utilities Index Mark 2 (HUI2) and Mark 3 (HUI3) in residents of long-term care (LTC) facilities, using a convergent/divergent validity approach, with the Functional Independence Measure (FIM), Quality of Life-Alzheimer's Disease (QOL-AD) and Resident Assessment Instrument (RAI) 2.0 as comparators.MethodsHealth status of 130 residents with dementia residing in one of seven LTC facilities was evaluated by their Healthcare Aides who were their primary care assistants. A priori hypotheses on the magnitude and direction of the correlations were formulated by two clinician/researchers and a researcher familiar with the measures and this population. Predicted and observed correlations were compared.ResultsMean overall HUI2 (0.48, SD 0.16) and HUI3 scores (0.31, SD 0.27) were indicative of severe disability. Of the 208 a priori hypotheses, 39.9% (n = 83) matched the observed correlations, 29.8% were underestimated and 19.7% were overestimated by one category.ConclusionsFindings support the use of the HUI2 and HUI3 in measuring health-related quality of life in dementia-related research to complement disease-specific measures.
The Creation and Validation of Active Cooperative Behavior Scales for Nurses
Authors: Alexander Matthew , Horan Kristin Alyssa 
Published: September 2021

Background and PurposeGeneral conflict management instruments are employed in healthcare settings despite their lack of focus on active and cooperative behaviors which are linked to positive outcomes. The purpose of this study is to create and validate active cooperative behavior scales for nurses.MethodsTwo convenience samples were collected via an online panel and a public registry of nurses to create and validate the new scales using factor analysis, and hierarchical regression in a nursing/healthcare sample.ResultsThe newly created active cooperative behavior scales (ACBS) showed acceptable internal consistency and explained more variance on job satisfaction than a commonly used compromising subscale.ConclusionsThe ACBS can assist currently practicing nurses and hospitals in identifying easily implementable behaviors, active listening, appropriate assertiveness, and brainstorming, that lead to effective conflict management.
Conducting Online Healthcare Research With Open-Ended Questions in the Age of COVID-19: A Critical Review
Authors: Ukuhor Hyacinth O. 
Published: September 2021

BackgroundWorldwide, there is a remarkable increase in Internet use, with a current penetration rate of 62%. This widespread Internet use and the global coronavirus disease (COVID-19) preventive measures provide opportunities for data collection using the Internet in healthcare research. No recent studies have been conducted regarding the methodological issues of asynchronous Internet research that employed opened-ended questions to explore providers’ and patients’ experiences.PurposeThis study utilizes prior research to explore methodological issues that affect online research using open-ended questions to obtain health data.MethodsThe electronic databases searched were PubMed, CINAHL, and full-text Ovid. Reference lists and the Journal of Medical Internet Research were manually searched. The search strategy was based on the PRISMA flow diagram. Articles published between January 2003 and May 2020 were searched. Inclusion criteria were asynchronous online researcher-led studies that used open-ended questions to explore healthcare issues. Methodological issues were extracted from the selected studies.ResultsThe evidence suggests that factors such as technical/website study/survey design issues, smartphone study applications, use of reminders, incentives, overrecruiting participants, using a combination of asynchronous and synchronous methods, trustworthiness, ethical and security issues affect the quality of data obtained in online health research.Implications for PracticeAsynchronous online research methods with open-ended questions could be used to collect high-quality data from patients, healthcare providers, and other participants in self-isolation, quarantine, and in diverse locations. However, researchers should be aware of the identified methodological issues. Future research could explore methodological issues and data quality in combined asynchronous and synchronous data collection methods.