bluuebunny/crossref_metadata_2025_split huggingface dataset:
Technical and financial feasibility for intensification of the extensive shrimp farming area in Mekong Delta, Vietnam
Authors: Schuur Anthonie M. , McNevin Aaron A. , Davis Robert P. , Boyd Claude E. , Brian Susanna , Tinh Huynh Quoc , Duy Nguyen Phuong 
Published: December 2021

AbstractEngineering design studies and financial feasibility assessments were made for construction, maintenance, equipment, operations, loan requirement, shrimp sales and predicted cash flows for simulated transformations of extensive shrimp farms to intensive shrimp farms in the Mekong Delta, Vietnam. Farms with less than 2.8 ha of feed‐based, aerated production ponds would not be profitable at shrimp yields less than 5 t/ha/year. Profit projections increased in response to greater production area on farms and larger annual pond yields. Farms with 6 ha or more of production ponds and yields of 10 t/ha/year or more had the best profit potential. Greater profitability at higher production intensity resulted from a reduction in the labour cost/product weight ratio as yield per unit area increased. Transformation from extensive to intensive farms would allow greater shrimp production, increase worker income and lessen land and water use. Environmental benefits would include a reduction in sediment starvation of the Mekong Delta, and the option to restore some of the several hundred thousand hectares of mangrove area severely degraded by extensive shrimp farming. These two improvements would be of benefit in retarding erosion along the Mekong Delta coastline. The possibility of intensification and consolidation of the shrimp farming area in the Mekong Delta deserves further consideration.
Acknowledging bereavement, strengthening communities: Introducing an online compassionate community initiative for the recognition of pandemic grief
Authors: Ummel Deborah , Vachon Mélanie , Guité‐Verret Alexandra 
Published: December 2021

AbstractDespite public health measures and collective efforts, millions of individuals have unfortunately died from COVID‐19 complications worldwide, leaving several million family members at risk of developing bereavement complications. In the Canadian province of Quebec, where substantial deaths were associated with COVID‐19, we established an online support community for bereaved caregivers who lost a loved one during the pandemic. We explain how we created a community that recognized pandemic grief and advocated for its wider acknowledgment. We discuss “compassionate communities,” the theoretical underpinning of our initiative, as a means to foster solidarity, normalize finitude, create and maintain a safe social space through group sharing, and challenging capitalist principles. We then describe the eight areas of activities inspired by the Charter of Pallium Canada: education and training, hospices and nursing homes, media and social media, commemoration, celebrations, artistic practices and storytelling, marginalized populations, and review and evaluate. We propose that online communities constitute a powerful space for community members to gather and advocate for greater awareness of the inequities found in end‐of‐life care and bereavement services, to denounce abusive situations experienced by many individuals who died from COVID‐19 complications, and to fight against the lack of recognition experienced by numerous caregivers.
Total Synthesis of (±)‐Taiwaniaquinol B through a Sequence of Fries Rearrangement and Photo‐Nazarov Cyclization
Authors: Chang Chieh‐Yu , Chen Chun‐Jen , Huang Sheng‐Hao , Kao Shih‐Chieh , Wu Yen‐Ku 
Published: December 2021

AbstractDescribed is a concise route to (±)‐taiwaniaquinol B. The 6‐5‐6‐fused ring system was constructed by sequencing a Fries rearrangement and a photo‐Nazarov cyclization. The application of photoirradiation conditions was found to be crucial for promoting the key electrocyclization and mitigating the competing oxo‐Michael pathway.
Hydrophobic Polysaccharides: Partially 2‐Deoxygenated Amyloses
Authors: Abe Shogo , Yamamoto Kazuya , Kadokawa Jun‐ichi 
Published: December 2021

AbstractIn this paper, we report that polysaccharide substrates bearing 2‐deoxygenated amylose chains are hydrophobic. A pure partially 2‐deoxygenated amylose was synthesized by the thermostable glucan phosphorylase‐catalyzed enzymatic copolymerization of d‐glucal, a non‐native monomer, with α‐d‐glucose 1‐phosphate, a native monomer from maltotriose, as a primer. A water contact angle on a cast film of the resulting polysaccharide confirms that it is hydrophobic. Enzymatic copolymerization using glycogen as a polymeric primer was used to investigate the possibility of engendering hydrophilic substrates with hydrophobicity by incorporating such polysaccharide chains; the resulting glycogen‐derived films with elongated partially 2‐deoxygenated amylose chains are strongly hydrophobic.
Are nonsteroidal anti‐inflammatory drugs effective enough for postoperative pain control after functional endoscopic sinus surgery and septoplasty? A randomized, controlled study
Authors: Saini Alok T. , Jiang Zi Y. , Starr Nicole C. , Talmadge Jason , Schmale Isaac , Radabaugh Paul , Yao William C. , Luong Amber U. , Citardi Martin J. 
Published: December 2021

AbstractBackgroundEndoscopic sinus surgery (ESS) and septoplasty are commonly performed procedures without standardized postoperative pain regimens. There is reluctance to prescribe opioids for postoperative pain given their potential for abuse. Nonsteroidal anti‐inflammatory drugs (NSAIDs) have been demonstrated to reduce or even obviate the need for opioid pain medications after otolaryngologic surgeries, but prospective validation is lacking.MethodsA randomized, controlled study comparing the efficacy of diclofenac sodium to hydrocodone/acetaminophen (APAP) after ESS with or without septoplasty was performed. Participants were given a 100‐mm visual analog pain scale (VAS) at postoperative days (PODs) 1, 2, 3, and 5 after ESS. Two‐sample t tests were used to compare pain scores between groups.ResultsOne hundred patients enrolled, and 74 patients provided pain scores to the survey. Pain was greatest for both groups on POD 1. Treatment with diclofenac sodium vs hydrocodone/APAP did not statistically impact pain scores at PODs 1, 2, 3, or 5. No cases of epistaxis requiring an emergency room visit or return to the operating room were noted during the study period.ConclusionDiclofenac sodium may be non‐inferior to hydrocodone/APAP in treating pain after ESS with or without septoplasty in opioid naive patients without pre‐existing pain conditions. Further studies with larger samples are warranted to investigate the potential superiority of diclofenac to hydrocodone/APAP in certain patients after ESS and septoplasty.
A Fluorescent Sensor for Quantitative Super‐Resolution Imaging of Amyloid Fibril Assembly**
Authors: Kaur Amandeep , Adair Liam D. , Ball Sarah R. , New Elizabeth J. , Sunde Margaret 
Published: December 2021

AbstractMany soluble proteins can self‐assemble into macromolecular structures called amyloids, a subset of which are implicated in a range of neurodegenerative disorders. The nanoscale size and structural heterogeneity of prefibrillar and early aggregates, as well as mature amyloid fibrils, pose significant challenges for the quantification of amyloid morphologies. We report a fluorescent amyloid sensor AmyBlink‐1 and its application in super‐resolution imaging of amyloid structures. AmyBlink‐1 exhibits a 5‐fold increase in ratio of the green (thioflavin T) to red (Alexa Fluor 647) emission intensities upon interaction with amyloid fibrils. Using AmyBlink‐1, we performed nanoscale imaging of four different types of amyloid fibrils, achieving a resolution of ≈30 nm. AmyBlink‐1 enables nanoscale visualization and subsequent quantification of morphological features, such as the length and skew of individual amyloid aggregates formed at different times along the amyloid assembly pathway.
Sustainable Superhydrophobic Surface with Tunable Nanoscale Hydrophilicity for Water Harvesting Applications
Authors: Wang Yi , Zhao Weinan , Han Mei , Guan Lu , Han Lian , Hemraj Afraz , Tam Kam Chiu 
Published: December 2021

AbstractSuperwettable surfaces show great potential in water harvesting applications, however, a scalable water harvesting surface remains elusive due to the trade‐off between water deposition and transport. Herein, we report a unique superhydrophobic surface with tunable nanoscale hydrophilicity constructed by structured Pickering emulsions. Preferential exposure of the cellulose nanocrystal's outer surface and wax microspheres accelerates droplet deposition allowing for the manipulation of droplet mobility. Appropriate tuning of the wetting characteristics of the surfaces, optimizing the hydrophobicity and density of the water affinity nanodomains enhance the water deposition rate without the sacrifice of water transport rate, achieving an optimal water harvesting flux of 3.402 L m−2 h−1 for a plate and 5.02 L m−2 h−1 for a mesh. This hydrophilic/superhydrophobic surface allows the controllable manipulation of droplet nucleation and removal to enhance the water harvesting efficiency.
Triplet Dynamic Nuclear Polarization of Guest Molecules through Induced Fit in a Flexible Metal–Organic Framework**
Authors: Fujiwara Saiya , Matsumoto Naoto , Nishimura Koki , Kimizuka Nobuo , Tateishi Kenichiro , Uesaka Tomohiro , Yanai Nobuhiro 
Published: December 2021

AbstractDynamic nuclear polarization utilizing photoexcited triplet electrons (triplet‐DNP) has great potential for room‐temperature hyperpolarization of nuclear spins. However, the polarization transfer to molecules of interest remains a challenge due to the fast spin relaxation and weak interaction with target molecules at room temperature in conventional host materials. Here, we demonstrate the first example of DNP of guest molecules in a porous material at around room temperature by utilizing the induced‐fit‐type structural transformation of a crystalline yet flexible metal–organic framework (MOF). In contrast to the usual hosts, 1H spin‐lattice relaxation time becomes longer by accommodating a pharmaceutical model target 5‐fluorouracil as the flexible MOF changes its structure upon guest accommodation to maximize the host–guest interactions. Combined with triplet‐DNP and cross‐polarization (CP), this system realizes an enhanced 19F NMR signal of guest target molecules.
Thermal‐Triggered Dynamic Disulfide Bond Self‐Heals Inorganic Perovskite Solar Cells
Authors: Zhang Qiaoyu , Duan Jialong , Guo Qiyao , Zhang Junshuai , Zheng Dengduan , Yi Fangxuan , Yang Xiya , Duan Yanyan , Tang Qunwei 
Published: December 2021

AbstractOne great challenge for perovskite solar cells (PSCs) lies in their poor operational stability under harsh stimuli by humidity, heat, light, etc. Herein, a thermal‐triggered self‐healing polyurethane (PU) is tailored to simultaneously improve the efficiency and stability of inorganic CsPbIBr2PSCs. The dynamic covalent disulfide bonds between adjacent molecule chains in PU at high temperatures self‐heal the in‐service formed defects within the CsPbIBr2perovskite film. Finally, the best device free of encapsulation achieves a champion efficiency up to 10.61 % and an excellent long‐term stability in an air atmosphere over 80 days and persistent heat attack (85 °C) over 35 days. Moreover, the photovoltaic performances are recovered by a simple heat treatment.
Interfacial Atom‐Substitution Engineered Transition‐Metal Hydroxide Nanofibers with High‐Valence Fe for Efficient Electrochemical Water Oxidation
Authors: Zhang Ben , Wu Zihe , Shao Wenjie , Gao Yun , Wang Weiwen , Ma Tian , Ma Lang , Li Shuang , Cheng Chong , Zhao Changsheng 
Published: December 2021

AbstractDeveloping low‐cost electrocatalysts for efficient and robust oxygen evolution reaction (OER) is the key for scalable water electrolysis, for instance, NiFe‐based materials. Decorating NiFe catalysts with other transition metals offers a new path to boost their catalytic activities but often suffers from the low controllability of the electronic structures of the NiFe catalytic centers. Here, we report an interfacial atom‐substitution strategy to synthesize an electrocatalytic oxygen‐evolving NiFeV nanofiber to boost the activity of NiFe centers. The electronic structure analyses suggest that the NiFeV nanofiber exhibits abundant high‐valence Fe via a charge transfer from Fe to V. The NiFeV nanofiber supported on a carbon cloth shows a low overpotential of 181 mV at 10 mA cm−2, along with long‐term stability (>20 h) at 100 mA cm−2. The reported substitutional growth strategy offers an effective and new pathway for the design of efficient and durable non‐noble metal‐based OER catalysts.
Thermal‐Triggered Dynamic Disulfide Bond Self‐Heals Inorganic Perovskite Solar Cells
Authors: Zhang Qiaoyu , Duan Jialong , Guo Qiyao , Zhang Junshuai , Zheng Dengduan , Yi Fangxuan , Yang Xiya , Duan Yanyan , Tang Qunwei 
Published: December 2021

AbstractOne great challenge for perovskite solar cells (PSCs) lies in their poor operational stability under harsh stimuli by humidity, heat, light, etc. Herein, a thermal‐triggered self‐healing polyurethane (PU) is tailored to simultaneously improve the efficiency and stability of inorganic CsPbIBr2 PSCs. The dynamic covalent disulfide bonds between adjacent molecule chains in PU at high temperatures self‐heal the in‐service formed defects within the CsPbIBr2 perovskite film. Finally, the best device free of encapsulation achieves a champion efficiency up to 10.61 % and an excellent long‐term stability in an air atmosphere over 80 days and persistent heat attack (85 °C) over 35 days. Moreover, the photovoltaic performances are recovered by a simple heat treatment.
Fractional‐order system identification based on an improved differential evolution algorithm
Authors: Yu Wei , Liang HengHui , Chen Rui , Wen Chenglin , Luo Ying 
Published: December 2021

AbstractWith the continuous increasing of system modeling accuracy and control performance requirements, the fractional‐order models are applied to describe the dynamic characteristics of the real systems more accurately over traditional integer‐order models. Traditional identification algorithms for fractional‐order system require stringent information on the derivatives of the objective function and initial values of the parameters, which is difficult in practical applications. The differential evolution (DE) algorithm has the advantage of simple implementation and superior performance for solving multi‐dimensional complex optimization problems which is suitable for the identification of fractional‐order systems. Aiming at improving the solution accuracy and convergence speed, an improved DE (IDE) algorithm for fractional‐order system identification is proposed. The movement strategy of the marine predator algorithm (MPA) and the mutation strategy of randomly selecting one from the optimal individual population as the basis vector are both adopted in this proposed IDE algorithm. The parameter information of the successfully mutated individual is archived during the iteration process. The scaling factor and crossover probability factor are adaptively adjusted according to the archived information to enhance the best solution accuracy obtained in the search process. By testing 13 sets of unimodal and multimodal functions with high‐performance optimization algorithms, the accuracy performance of the proposed IDE solution is verified. The IDE algorithm is applied to identify the parameters of the fractional‐order system of permanent magnet synchronous motor with simulation and experiment. The identification results clearly show the effectiveness of the proposed methodology.
Structural analysis of random transgene integration in CHO manufacturing cell lines by targeted sequencing
Authors: Stadermann Anna , Gamer Martin , Fieder Jürgen , Lindner Benjamin , Fehrmann Steffen , Schmidt Moritz , Schulz Patrick , Gorr Ingo H. 
Published: December 2021

AbstractGenetically modified CHO cell lines are traditionally used for the production of biopharmaceuticals. However, an in‐depth molecular understanding of the mechanism and exact position of transgene integration into the genome of pharmaceutical manufacturing cell lines is still scarce. Next‐generation sequencing (NGS) holds great promise for strongly facilitating the understanding of CHO cell factories, as it has matured to a powerful and affordable technology for cellular genotype analysis. Targeted Locus Amplification (TLA) combined with NGS allows for robust detection of genomic positions of transgene integration and structural genomic changes occurring upon stable integration of expression vectors. TLA was applied to generate comparative genomic fingerprints of several CHO production cell lines expressing different monoclonal antibodies. Moreover, high producers resulting from an additional round of transfection of an existing cell line (supertransfection) were analyzed to investigate the integrity and the number of integration sites. Our analyses enabled detailed genetic characterization of the integration regions with respect to the number of integrates and structural changes of the host cell's genome. Single integration sites per clone with concatenated transgene copies could be detected and were in some cases found to be associated with genomic rearrangements, deletions or translocations. Supertransfection resulted in an increase in titer associated with an additional integration site per clone. Based on the TLA fingerprints, CHO cell lines originating from the same mother clone could clearly be distinguished. Interestingly, two CHO cell lines originating from the same mother clone were shown to differ genetically and phenotypically despite their identical TLA fingerprints. Taken together, TLA provides an accurate genetic characterization with respect to transgene integration sites compared with conventional methods and represents a valuable tool for a comprehensive evaluation of CHO production clones early in cell line development.
Enhancing tryptophan production by balancing precursors in Escherichia coli
Authors: Guo Liang , Ding Shuang , Liu Yadi , Gao Cong , Hu Guipeng , Song Wei , Liu Jia , Chen Xiulai , Liu Liming 
Published: December 2021

AbstractTryptophan, an essential aromatic amino acid, is widely used in animal feed, food additives, and pharmaceuticals. Although sustainable and environmentally friendly, microbial tryptophan production from renewable feedstocks is limited by low biosynthesis and transport rates. Here, an Escherichia coli strain capable of efficient tryptophan production was generated by improving and balancing the supply of precursors and by engineering membrane transporters. Tryptophan biosynthesis was increased by eliminating negative regulatory factors, blocking competing pathways, and preventing tryptophan degradation. Promoter engineering balanced the supply of the precursors erythrose‐4‐phosphate and phosphoenolpyruvate, as well as the availability of serine. Finally, the engineering of tryptophan transporters prevented feedback inhibition and growth toxicity. Fed‐batch fermentation of the final strain (TRP12) in a 5 L bioreactor produced 52.1 g·L−1 of tryptophan, with a yield of 0.171 g·g−1 glucose and productivity of 1.45 g·L−1·h−1. The metabolic engineering strategy described here paves the way for high‐performance microbial cell factories aimed at the production of tryptophan as well as other valuable chemicals.
The potential benefit of leptin therapy against amyotrophic lateral sclerosis (ALS)
Authors: Ferrer‐Donato Agueda , Contreras Ana , Fernandez Paloma , Fernandez‐Martos Carmen M. 
Published: December 2021

AbstractBackgroundTargeting leptin could represent a rational strategy to treat amyotrophic lateral sclerosis (ALS), as previously clinical studies have shown its levels to be associated with a lower risk of ALS disease. However, very little is known about the potential influence of leptin in altering disease progression in ALS, as it has thus far been correlated with the protection exerted by increased fat mass stores.MethodsWe studied the impact of leptin treatment beginning at 42‐days of age (asymptomatic stage of disease) in the TDP‐43 (TDP43A315T) transgenic (Tg) ALS mouse model.ResultsOur study shows that leptin treatment was associated with altered expression of adipokines and metabolic proteins in TDP43A315T mice. We also observed that weight loss decline was less prominent after leptin treatment in TDP43A315T mice relative to vehicle‐treated animals. In TDP43A315T mice treated with leptin the disease duration lasted longer along with an improvement in motor performance relative to vehicle‐treated animals.ConclusionsCollectively, our results support leptin as a potential novel treatment approach for ALS.
Early implementation of a perioperative nutrition support pathway for patients undergoing esophagectomy for esophageal cancer
Authors: Carr Rebecca A. , Harrington Caitlin , Stella Christina , Glauner Diana , Kenny Erin , Russo Lianne M. , Garrity Meghan J. , Bains Manjit S. , Sihag Smita , Jones David R. , Molena Daniela 
Published: December 2021

AbstractBackgroundUnintentional weight loss and malnutrition are associated with poorer prognosis in patients with cancer. Risk of cancer‐associated malnutrition is highest among patients with esophageal cancer (EC) and has been repeatedly shown to be an independent risk factor for worse survival in these patients. Implementation of nutrition protocols may reduce postoperative weight loss and enhance recovery in these patients.MethodsWe retrospectively identified all patients who underwent Ivor Lewis esophagectomy for EC from January 2015 to August 2019 from a prospectively collected institutional database. Patients who underwent surgery after the implementation of this protocol (September 2017–August 2019) were compared with patients who underwent resection before protocol implementation (January 2015–July 2017). Patients undergoing surgery during the month of protocol initiation were excluded.ResultsOf the 404 patients included in our study, 217 were in the preprotocol group, and 187 were in the postprotocol group. Compared with the preprotocol group, there were significant reductions in length of hospital stay (p < 0.001), time to diet initiation (p < 0.001), time to feeding tube removal (p = 0.012), and postoperative weight loss (p = 0.002) in the postprotocol group. There was no significant difference in the incidence of postoperative complications, 30‐day readmission, or mortality rates between groups.ConclusionsResults of the present study suggest a standardized perioperative nutrition protocol may prevent unintentional weight loss and improve postoperative outcomes in patients with EC undergoing resection.
Psychotherapists’ psychological well‐being: The role of epistemic orientation and emotion regulation
Authors: Palma Emanuel Missias Silva , Gondim Sônia Maria Guedes 
Published: December 2021

AbstractPsychotherapists’ mental health has been traditionally associated with the harmful consequences of clinical practice. In contrast to this dominant model, a growing body of research in positive psychology has sparked an interest in therapists’ positive well‐being. The main objective of this study was to examine the relations between therapists’ epistemic orientation modes, emotion regulation strategies and psychological well‐being (PWB). This was a cross‐sectional study in which participants were 674 therapists (78.5% women), with a mean age of 42.02 years (SD = 13.06). Therapists completed an online questionnaire containing self‐report measures of all study variables. Structural equation modelling using latent variables was performed to test the study hypotheses. Results revealed no significant direct effects of epistemic orientation on PWB. Up‐ and downregulation strategies had the most substantial direct effects on total PWB. Furthermore, emotion regulation mediated the association between therapists’ epistemic orientation modes and PWB, accounting for 57% of the variance in the outcome variable. Implications for training, practice and therapist self‐care are discussed.