bluuebunny/crossref_metadata_2025_split huggingface dataset:
Field blood preservation and DNA extraction from wild mammals: methods and key factors for biodiversity studies
Authors: Carvajal-Agudelo Juan D. , Trujillo-Betancur M. Paula , Velásquez-Guarín Daniela , Ramírez-Chaves Hector E. , Pérez-Cárdenas Jorge E. , Rivera-Páez Fredy A. 
Published: June 2021

Studies on public health and wild mammal biodiversity include a genetic component. For blood samples, there must be optimal sample collection conditions since these can affect DNA preservation and extraction. This study evaluated the use of liquid and dry DNA preservation methods and commercial and non-commercial DNA extraction methods on field-collected blood samples. For this, 264 total blood samples were collected from wild mammals. A first group of samples was preserved in guanidine hydrochloride (GuHCl) and DNA was extracted using six commercial kits: Bioline, Norgen, Invitrogen, Promega, and Qiagen, in addition to phenol-chloroform isoamyl alcohol (PC) and guanidine thiocyanate (GIT). Another group of samples was preserved in Whatman® FTA® cards and DNA was extracted with PC and GIT. The extractions with GIT and PC showed the highest values (ng/µL) and variation in DNA concentration, while the commercial kit showed low variation. Sample preservation in Whatman® FTA® cards provided low variation and quantity of the extracted DNA compared with the use of GuHCl. Concerning DNA quality, the commercial kits yielded higher purity, while GIT and PC-based protocols provided highly variable results. Furthermore, the use of GIT and PC yielded a higher amount of DNA, yet, of variable quality. Overall, extraction based on commercial kits and Whatman® FTA® preservation allowed obtaining more standardized DNA qualities and quantities.
AN ANALYSIS OF TENTH GRADE STUDENTS’ DIFFICULTIES IN COMPREHENDING RECOUNT TEXT
Authors: Prayitno Eko , Sartika Dewi , Asmara Rekha 
Published: June 2021

This study aimed to recognize the problems faced by the tenth graders of SMA Negeri 1 Jejawi and to determine the factors that influenced students’ difficulties in comprehending recount text. This study was a qualitative research. The subject of the study was the tenth grade students of SMA Negeri 1 Jejawi in academic year of 2019/2020. The techniques of collecting the data were interview and reading comprehension test. To analyses the data, reducing, data displayed, content analysis, and draw conclusion were applied. The result of the study showed that there were 68.7% students had difficulties in finding the main idea, 70.15% students had difficulties in understanding vocabulary, 66% students had difficulties in finding reference, 59.3% students had difficulties in finding purpose of the text, 67.24% students had difficulties in finding detail information, and 81.95% students had difficulties in determining the generic structure of the text. Some factors influenced students’ difficulties in comprehending recount text were material that has long passage, limitation of words, learners’ background, and environment.
Accuracy analysis of BLE beacon-based localization in smart buildings
Authors: Ivanov Rosen 
Published: June 2021

The majority of services that deliver personalized content in smart buildings require accurate localization of their clients. This article presents an analysis of the localization accuracy using Bluetooth Low Energy (BLE) beacons. The aim is to present an approach to create accurate Indoor Positioning Systems (IPS) using algorithms that can be implemented in real time on platforms with low computing power. Parameters on which the localization accuracy mostly depends are analyzed: localization algorithm, beacons’ density, deployment strategy, and noise in the BLE channels. An adaptive algorithm for pre-processing the signals from the beacons is proposed, which aims to reduce noise in beacon’s data and to capture visitor’s dynamics. The accuracy of five range-based localization algorithms in different use case scenarios is analyzed. Three of these algorithms are specially designed to be less sensitive to noise in radio channels and require little computing power. Experiments conducted in a simulated and real environment show that using proposed algorithms the localization accuracy less than 1 m can be obtained.
Evaluation of blackcurrant (Ribes nigrum) germplasm structure by microsatellite-based fingerprinting for the diversification of the breeding material
Authors: Lācis Gunārs , Kārkliņa Katrīna , Kota-Dombrovska Irita , Strautiņa Sarmīte 
Published: June 2021

BACKGROUND: Blackcurrants are an important berry crop whose further development depends mainly on the diversity of available plant material and its successful inclusion in the breeding. OBJECTIVE: The study aimed to evaluate selected SSR markers’ applicability for the analysis of germplasm consisting of genotypes developed through complicated multistage crosses among different Ribes species and estimate the genetic structure and level of genetic diversity of the blackcurrant collection. METHODS: The set of 110 blackcurrant accessions from the Latvian genetic resources collection was genotyped using 18 previously described SSR markers. RESULTS: The marker set provided all genotypes with unique fingerprints and proved the need for reference genotypes in international studies for data validation. The accessions clustered according to pedigree and did not group by country of origin or breeding programme. CONCLUSIONS: The tested SSR markers uncovered relationships between blackcurrant accessions of complicated interspecific composition and revealed an extensive mutual exchange of germplasm among breeding programmes, thus pointing to the need for new donors of valuable characteristics. The results also highlighted the necessity to identify each species’ proportion in the hybrid material to assess the impact of species-linked gene expression.
ПРИНЦИП ПОЄДНАННЯ ФУНКЦІЙ ЕЛЕМЕНТІВ КОНСТРУКЦІЙ МОДИФІКАЦІЙ ПОВІТРЯНИХ СУДЕН І ЙОГО СКЛАДОВІ
Authors: Серебрянникова В. Ю. 
Published: June 2021

It is shown that the aviation business system determines the relationship between the producer of aircraft (AC) of the transport category and the airlines operating them, includes an order for the creation and delivery of aircraft and their modifications, as well as support documentation at all stages of the life cycle.The main requirements and conditions for the effective fulfillment of an operator's order for aircraft modifications have been synthesized, the implementation of which significantly contributes to the implementation of the principle of combining the functions of units and assemblies of aircraft modifications.The synthesized particular principles have, apparently, many ways of their constructive and technological implementation, the main of which are:- rational choice of appropriate construction materials;- giving the elements additional displacements;- use of the principle of reversibility with the appropriate structural and technological modification of the elements;- special arrangement of elements;-changing the structure and shape of elements.Combining the function of the elements is rational if, as a result of functional summation, mutual functional amplification, interference or superposition of gains, the efficiency of the combined element , determined by its functional property or their combination with the regulated reliability of the element in the aircraft system, is higher than the functional efficiencies of the original elements.Since the combination of the functions of the elements is essentially one of the means of increasing the efficiency of the aircraft, it does not introduce fundamental features into the formulation of the mathematical problem of the optimal design of the aircraft and the units of its modifications, however, the formulated principles of combining the properties of the elements and the condition for the rationality of the combination of functions to a certain extent outline the main problems search for rational design and technological solutions for multifunctional elements of the aircraft. These problems, first of all, should include the development of the theoretical foundations of general and specific methods that establish the areas of optimal application of the formulated principles of preparation for production of aircraft modifications related to ensuring the quality of products, their manufacturability and reliability
A Bioética, seu desenvolvimento e importância para as Ciências da Vida e da Saúde
Authors: Schramm Fermin Roland 
Published: June 2021

O artigo apresenta, em grandes linhas, a bioética, seu desenvolvimento e sua possível utilidade para enfrentar os problemas que se colocam à pesquisa que envolve seres humanos, tendo em conta simultaneamente as exigências disciplinares e metodológicas da bioética e algumas das necessidades da ética em pesquisa da própria instituição que é o INCA.
Efektivitas Penggunaan Website pada Ibu Nifas sebagai Inovasi Baru Media Edukasi Gizi tentang Pemberian Makanan Bayi dan Anak di Ruang Rawat Inap Peristi RSUD Sidoarjo
Authors: Astutik Pudji , Rohmawati Rosida Indriani , Sulistyowati Pipit 
Published: June 2021

Untuk mengetahui efektivitas penggunaan media website pada ibu nifas sebagai inovasi baru media edukasi gizi tentang pemberian makanan bayi dan anak di Ruang Rawat Inap Peristi RSUD Kabupaten Sidoarjo.Penelitian ini menggununakan studi kuasi eksperimental, pre-test dan post test intervention. Teknik pengambilan sampel pada penelitian ini menggunakan total sampling yang berarti jumlah sampel sama dengan jumlah populasi yaitu 32 orang pada masing-masing kelompok. Hasil penelitian menunjukkan bahwa terdapat perbedaan yang signifikan antara tingkat pengetahuan ibu nifas sebelum dan sesudah diberikan edukasi mengenai Pemberian Makan Bayi dan Anak baik melalui leaflet (p=0,050) website (P=0,001). Hasil uji beda skor pengetahuan ibu antara kelompok leaflet dan website menunjukkan bahwa tidak terdapat perbedaan yang signifikan (P=0,367). Website dapat digunakan sebagai alternatif media edukasi mengenai Pemberian Makan Bayi dan Anak pada ibu nifas.
Opini Mahasiswa Terhadap Keberadaan Permukiman Kampung Kumuh Stren Kali Di Kota Surabaya
Authors: Ganefwati Rini , Nurlita Ita , Fitriawardhani Tira 
Published: June 2021

Fenomena kontroversial terhadap eksistensi kampung kumuh di stren kali di perkotaan masih selalu menjadi problem hangat di perkotaan dalam hampir decade setiap pimpinan dan tak kunjung tuntas penyelesaiannya. Mengingat perkembangan kampung kumuh dalam puluhan tahun selalu makin padat. Karena itu mahasiswa sebagai generasi muda, yang kelak mempunyai akses untuk menambah jumlah tempat tinggal di perkampungan kumuh stren kali tersebut. Maka tujuan penelitian ini hendak mengetahui bagaimana opini mahasiswa tentang keberadaan kelangsungan kampung kumuh stren kali. Dengan mengunakan metodologi survai yang diharapkan bisa menggali secara objektif bagaimana opini mahasiswa sebagai generasi muda dalam menyikapi problema kampung kumuh di perkotaan, dengan tehnik analisa deskriptif kuantitatif, yang menggunakan data prosentase dalam bentuk diagram lingkaran. Dengan lokasi penelitian di Universitas Bhayangkara Surabaya. Dan hasil penelitian yang kami peroleh bahwa opini mahasiswa terhadap permukiman kampung kumuh stren kali adalah tidak mendukung keberadaan permukiman kampung kumuh di stren kali, karena bukan merupakan tempat tinggal yang nyaman.
Andata e ritorno per il diritto di asilo costituzionale
Authors: Benvenuti Marco 
Published: June 2021

Sommario: Prologo1. La "svolta storica nella lotta all'immigrazione clandestina", ovvero della crisi del c.d. "sistema asilo" alla luce della recente pratica dei respingimenti in mare2. L'"identità di natura giuridica del diritto alla protezione umanitaria, del diritto allo status di rifugiato e del diritto costituzionale di asilo", ovvero della funzione emulativa del diritto di asilo costituzionale3. "La presenza del richiedente il diritto di asilo nel territorio dello Stato non č condizione necessaria per il conseguimento del diritto stesso", ovvero della funzione effettiva del diritto di asilo costituzionale
L'azione civile contro la discriminazione: rassegna giurisprudenziale dei primi dieci anni
Authors: Casadonte Annamaria , Guariso Alberto 
Published: June 2021

Sommario:1. L'evoluzione normativa2. Il diritto alla parità di trattamento nella cornice dei diritti fondamentali3. Il "combinato disposto" degli artt. 43 TU n. 286/1998 e 2-3 d.lgs. 215/20034. La casistica giurisprudenziale: a) la c.d. emergenza nomadi; b) cittadinanza e lavoro pubblico5. I profili processuali: a) natura del procedimento; b) giurisdizione; c) legittimazione ad agire; d) interesse ad agire; e) poteri del giudice e provvedimento conclusivo: inibitoria e risarcimento; f) impugnazione
La procedura di proroga del trattenimento alla verifica di legittimità
Authors: Savio Guido 
Published: June 2021

Sommario: Introduzione1. L'evoluzione normativa della tutela del diritto di difesa nelle procedure di trattenimento e allontanamento2. La motivazione delle sentenze in esame3. Le conseguenze pratiche della necessità di celebrare l'udienza di proroga dei trattenimenti
Diritto italiano: Asilo
Authors:   Redazione
Published: June 2021

1. Tribunale di Torino 29.5.2009 n. 177 - impugnazione del diniego del riconoscimento dello status di rifugiato e protezione sussidiaria - carenza di legittimazione passiva del Ministero dell'interno - accertamento della verosimiglianza e attendibilità dei fatti allegati2. Tribunale di Palermo 31.8.2009 n. 187 - impugnazione del diniego del riconoscimento dello status di rifugiato e richiesta subordinata di protezione sussidiaria - accertamento di merito del giudice ordinario - onere probatorio del richiedente3. Tribunale amministrativo regionale Sicilia 23.10.2009 n. 1702 - impugnazione del silenzio rifiuto sulla istanza di permesso di soggiorno per motivi umanitari - accertamento di merito del giudice ordinario - inammissibilità del ricorso proposto avanti al Tar4. Tribunale amministrativo regionale Lazio 19.2.2010 n. 2596 - cittadino extracomunitario richiedente asilo - provvedimento di trasferimento in Grecia quale Stato membro di ingresso - violazione della raccomandazione UNHCR in merito alla carenza di tutela esistente in Grecia - carenza di motivazione in ordine alla individuazione del Paese terzo sicuro
Osservatorio europeo: Documenti ; Segnalazioni
Authors:   Redazione
Published: June 2021

1. Regolamento (UE) n. 265/2010 del Parlamento europeo e del Consiglio, del 25.3.2010, che modifica la Convenzione di applicazione dell'accordo di Schengen e il Regolamento (CE) n. 562/2006 - Circolazione dei titolari di visto per soggiorni di lunga durata2. Decisione (UE) n. 252/2010 del Consiglio, del 26.4.2010, che integra il codice frontiere Schengen - Sorveglianza delle frontiere marittime esterneSegnalazioni
Un mercato europeo per gli slots aeroportuali: le prospettive del secondary trading
Authors: Colangelo Margherita 
Published: June 2021

Negli ultimi anni probabilmente il problema piů rilevante che mina l'efficienza del settore aeroportuale riguarda l'insufficienza di slots aeroportuali per soddisfare l'intera domanda da parte delle compagnie aeree. Č generalmente condiviso che un'allocazione efficiente dell'esistente scarsa capacità, e dunque degli slots, puň costituire uno strumento per gestire lo squilibrio tra domanda ed offerta. Gli esperti concordano nel ritenere che l'attuale procedura di allocazione, in particolare ildegli slots, costituisca un'importante causa di inefficienza del mercato aereo europeo e indicano, come soluzione potenzialmente efficiente, l'adozione di meccanismi di mercato, in particolare l'introduzione di un mercato secondario. Con la Comunicazione del 30 Aprile 2008 la Commissione Europea ha per la prima volta riconosciuto la legittimità dello.
Model Bimbingan dan Konseling Islami: Analisis Pemikiran Hasan Langgulung
Authors: Setiawan Setiawan 
Published: June 2021

<p class="15bIsiAbstractBInggris">Guidance and counselling are an integral part of education, but its existence in educational institutions in the west Bogor district has not been as expected. This research aims to give birth to an alternative model of the concept of guidance and counselling at the junior high school level. The development leads to the concept of guidance and counselling, which includes objectives, foundations, principles, methods and techniques, and the philosophy of guidance and counselling. The approach used in this research is library research and field research. The first approach is collecting books - related books, both primary and secondary, scientific journals on Islamic counselling guidance. The analysis method used is the skin method as the design of the model. The results of field observations turned out to be guidance and counselling that has not been under expectations because there is a partial system and paradigm that must be improved. The study results that the model of guidance and counselling should be integral, holistic in directing learners to achieve the happiness of the world and the hereafter. During this image in guidance and counselling, it is only to take care of people who directly have problems. Whereas guidance and counselling are more than, it can be seen from the function of guidance and counselling.</p><p class="16aJudulAbstrak"><strong>Abstrak</strong></p><p class="16bIsiAbstrak">Bimbingan dan konseling adalah bagian yang tidak terpisahkan dari dunia pendidikan, akan tetapi keberadaannya di lembaga Pendidikan di daerah kabupaten Bogor Barat belum sesuai harapan. Fokuskan penelitian ini adalah melahirkan model alternatif konsep bimbingan dan konseling tingkat sekolah menengah pertama/ SLTP. Pengembangan tersebut mengarah pada konsep bimbingan dan konseling yang meliputi tujuan, landasan, prinsip-prinsip, metode dan teknik serta falsafah bimbingan dan konseling. Pendekatan yang digunakan dalam penelitian ini adalah library research dan field research. pendekatan yang pertama, tahapan awal mengumpulkan buku – buku terkait baik yang primer maupun yang sekunder, jurnal ilmiah tentang bimbingan konseling Islami. metode analisis yang digunakan adalah Metode kualitatif sebagai perancangan model. Hasil observasi lapangan ternyata bimbingan dan konseling yang selama ini ternyata belum sesuai harapan dikarenakan ada sistem dan paradigma yang parsial yang harus dibenahi. Hasil penelitian bahwa model bimbingan dan konseling itu harus bersifat holistik integral dalam mengarahkan peserta didik untuk meraih kebahagiaan dunia dan akhirat Selama ini image di bimbingan dan konseling itu hanya untuk mengurusi orang - orang yang bermasalah secara langsung. Padahal keberadaan bimbingan dan konseling lebih dari itu, bisa dilihat dari fungsi tujuan bimbingan dan konseling.</p>
Konsep Pendidikan Adab dalam Kitab Al-Jâmi’ Al-Ṣaḥîḥ Karya Imam Al-Tirmiżî dan Relevansinya dengan Tujuan Pendidikan Nasional
Authors: Haryanto Haryanto 
Published: June 2021

<p class="15bIsiAbstractBInggris">The character education program launched by the Government has not reaped encouraging results, as evidenced by the worsening moral decline that occurs in the midst of the nation's children, such as corruption, drug abuse, LGBT lifestyle, free sex association, fighting between students, students and villagers, intolerance among religious people, separatism that threatens the integrity of the Republic of Indonesia, and so forth. The concept of education in imam al-Tirmiżî's al-Jâmi' al-Ṣaḥîḥ explains in detail the basic potential of man (heart, reason, taste, initiative and body), and explains the relationship between an individual and religious principles, with oneself and with his environment. The concept is very relevant to the purpose of national education, which is noble and morally praiseworthy to be able to improve the quality of individuals, families and communities to build a just and civilized national and state order, to further achieve the ideals of social justice for all Indonesians.</p><p class="16aJudulAbstrak"><strong>Abstrak </strong> </p><p class="16cKataKunci">Program pendidikan karakter yang dicanangkan oleh Pemerintah belum menuai hasil yang menggembirakan, dibuktikan dengan semakin parahnya kemunduran moral yang terjadi di tengah-tengah anak bangsa, seperti korupsi, penyalahgunaan obat-obat terlarang, gaya hidup LGBT, pergaulan seks bebas, tawuran antar pelajar, mahasiswa dan warga kampung, intoleransi antar umat beragama, separatisme yang mengancam keutuhan NKRI, dan lain sebagainya. Konsep pendidikan adab dalam kitab al-Jâmi’ al-Ṣaḥîḥ karya Imam al-Tirmiżî menerangkan secara terperinci dalam mengembangkan potensi dasar manusia (hati, akal, rasa, karsa dan raga), serta menjelaskan relasi antara seorang individu dengan prinsip-prinsip agama, dengan diri sendiri dan dengan lingkungannya. Konsep sangat relevan dengan tujuan pendidikan nasional, yangmana adab mulia dan akhlak terpuji mampu meningkatkan kualitas individu, keluarga dan masyarakat untuk membangun tatanan berbangsa dan bernegara yang berkeadilan dan beradab, untuk selanjutnya mencapai cita-cita keadilan sosial bagi seluruh rakyat Indonesia.</p>
Kemampuan Kimia dan Matematika Siswa MAN 2 Kota Jambi: Relasi dalam Fakta
Authors: Rosmalinda Desy 
Published: June 2021

Sama halnya dengan Biologi dan Fisika, kimia merupakan cabang ilmu alam yang harus dikuasai siswa. Banyak hal yang menjadi kendala siswa selama mempelajari kimia, termasuk saat mengerjakan soal-soal kimia yang mengandung perhitungan matematis. Penelitian ini bertujuan untuk mengetahui kemampuan kimia dan matematika siswa Madrasah Aliyah dalam konteks relasi sesuai fakta di lapangan. Jenis penelitian ini yaitu deskriptif kualitatif. Pengumpulan data dilakukan dengan menggunakan tes tertulis dan juga wawancara. Hasil penelitian menunjukkan bahwa siswa yang tidak bisa menjawab soal kimia dengan benar, ternyata bisa menjawab soal matematika yang memiliki operasi matematis yang sama dengan soal kimia tersebut. Bahkan dari hasil wawancara diperoleh informasi bahwa lebih dari 50% siswa mengalami kendala memahami konsep. Dari penelitian ini didapat beberapa fakta menarik. Fakta pertama, siswa yang memiliki kemampuan kimia rendah ternyata tidak selalu memiliki kemampuan matematika yang lemah. Fakta kedua, kendala yang dihadapi banyak siswa dalam mempelajari kimia yaitu sulitnya memahami konsep. Fakta ketiga, metode mengajar guru juga menentukan tingkat ketertarikan siswa untuk mempelajari kimia yang pada akhirnya juga berpengaruh terhadap kemampuan kimia siswa. Fakta keempat, minat siswa untuk mempelajari kimia menjadi faktor internal siswa untuk dapat meningkatkan kemampuan kimianya. Fakta kelima, rajin berlatih mengerjakan soal kimia merupakan salah satu cara mengasah kemampuan kimia. Dengan demikian kemampuan matematika siswa tidak menjadi pengaruh utama terhadap kemampuan kimia siswa.
PENERAPAN LEARNING CYCLE 5E MELALUI PETA PIKIR MENINGKATKAN HASIL BELAJAR PEMAHAMAN KONSEP DAN HASIL BELAJAR KOGNITIF SISWA KELAS V PADA PEMBELAJARAN IPA
Authors: Harneli Muslima Harneli , Koto Irwan Koto , Winarni Endang Widi 
Published: June 2021

Model pembelajaran learning cycle 5E melalui peta pikir digunakan untuk masalah pemahaman konsep dan hasil belajar kognitif siswa pada pembelajaran IPA kelas V SD Negeri 32 Bengkulu Tengah melalui Penelitian Tindakan Kelas (PTK) sebanyak 3 siklus dalam penerapannya. Metode pengumpulan data dilakukan dengan observasi dan tes menggunakan lembar observasi keterlaksanaan pembelajaran model learning cycle 5E melalui peta pikir oleh guru siswa. Penilaian pemahaman konsep menggunakan instrumen pembuatan peta pikir dan hasil tes ketuntasan belajar klasikal dianalisis secara deskriptif kuantitatif. Hasil penelitian ini menunjukkan bahwa penerapan model Learning Cycle 5E Melalui Peta Pikir untuk meningkatkan aktivitas proses pembelajaran, pemahaman konsep, dan hasil belajar kognitif siswa pada pembelajaran IPA kelas V di SD Nengeri 32 Bengkulu Tengah.