bluuebunny/crossref_metadata_2025_split huggingface dataset:
Spatial-ATAC-seq: spatially resolved chromatin accessibility profiling of tissues at genome scale and cellular level
Authors: Deng Yanxiang , Bartosovic Marek , Ma Sai , Zhang Di , Liu Yang , Qin Xiaoyu , Su Graham , Xu Mina L. , Halene Stephanie , Craft Joseph E. , Castelo-Branco Gonçalo , Fan Rong 
Published: June 2021

AbstractCellular function in tissue is dependent upon the local environment, requiring new methods for spatial mapping of biomolecules and cells in the tissue context. The emergence of spatial transcriptomics has enabled genome-scale gene expression mapping, but it remains elusive to capture spatial epigenetic information of tissue at cellular level and genome scale. Here we report on spatial-ATAC-seq: spatially resolved chromatin accessibility profiling of tissue section via next-generation sequencing by combining in situ Tn5 transposition chemistry and microfluidic deterministic barcoding. Spatial chromatin accessibility profiling of mouse embryos delineated tissue region-specific epigenetic landscapes and identified gene regulators implicated in the central nerve system development. Mapping the accessible genome in human tonsil tissue with 20μm pixel size revealed spatially distinct organization of immune cell types and states in lymphoid follicles and extrafollicular zones. This technology takes spatial biology to a new realm by enabling spatially resolved epigenomics to improve our understanding of cell identity, state, and fate decision in relation to epigenetic underpinnings in development and disease.
Heme oxygenase-2 (HO-2) binds and buffers labile heme, which is largely oxidized, in human embryonic kidney cells
Authors: Hanna David A. , Moore Courtney M. , Liu Liu , Yuan Xiaojing , Fleischhacker Angela S. , Hamza Iqbal , Ragsdale Stephen W. , Reddi Amit R. 
Published: June 2021

AbstractHeme oxygenases (HO) detoxify heme by oxidatively degrading it into carbon monoxide, iron, and biliverdin, which is reduced to bilirubin and excreted. Humans express two isoforms: inducible HO-1, which is up-regulated in response to various stressors, including excess heme, and constitutive HO-2. While much is known about the regulation and physiological function of HO-1, comparatively little is known about the role of HO-2 in regulating heme homeostasis. The biochemical necessity for expressing constitutive HO-2 is largely dependent on whether heme is sufficiently abundant and accessible as a substrate under conditions in which HO-1 is not induced. By measuring labile heme, total heme, and bilirubin in human embryonic kidney HEK293 cells with silenced or over-expressed HO-2, and various HO-2 mutant alleles, we found that endogenous heme is too limiting to support HO-2 catalyzed heme degradation. Rather, we discovered that a novel role for HO-2 is to bind and buffer labile heme. Taken together, in the absence of excess heme, we propose that HO-2 regulates heme homeostasis by acting as a heme buffering factor in control of heme bioavailability. When heme is in excess, HO-1 is induced and both HO-2 and HO-1 can provide protection from heme toxicity by enzymatically degrading it. Our results explain why catalytically inactive mutants of HO-2 are cytoprotective against oxidative stress. Moreover, the change in bioavailable heme due to HO-2 overexpression, which selectively binds ferric over ferrous heme, is consistent with the labile heme pool being oxidized, thereby providing new insights into heme trafficking and signaling.
A numerical solution to thermo‐mechanical behavior of temperature dependent rotating functionally graded annulus disks
Authors: Madan Royal , Bhowmick Shubhankar 
Published: June 2021

Purpose
The purpose of this study is to investigate Thermo-mechanical limit elastic speed analysis of functionally graded (FG) rotating disks with the temperature-dependent material properties. Three different material models i.e. power law, sigmoid law and exponential law, along with varying disk profiles, namely, uniform thickness, tapered and exponential disk was considered.

Design/methodology/approach
The methodology adopted was variational principle wherein the solution was obtained by Galerkin’s error minimization principle. The Young’s modulus, coefficient of thermal expansion and yield stress variation were considered temperature-dependent.

Findings
The study shows a substantial increase in limit speed as disk profiles change from uniform thickness to exponentially varying thickness. At any radius in a disk, the difference in von Mises stress and yield strength shows the remaining stress-bearing capacity of material at that location.

Practical implications
Rotating disks are irreplaceable components in machinery and are used widely from power transmission assemblies (for example, gas turbine disks in an aircraft) to energy storage devices. During operations, these structures are mainly subjected to a combination of mechanical and thermal loadings.

Originality/value
The findings of the present study illustrate the best material models and their grading index, desired for the fabrication of uniform, as well as varying FG disks. Finite element analysis has been performed to validate the present study and good agreement between both the methods is seen.
Optimized resource-constrained method for project schedule compression
Authors: Elkabalawy Moaaz , Moselhi Osama 
Published: June 2021

PurposeThis paper aims to present an integrated method for optimized project duration and costs, considering the size and cost of crews assigned to project activities' execution modes.Design/methodology/approachThe proposed method utilizes fuzzy set theory (FSs) for modeling uncertainties associated with activities' duration and cost and genetic algorithm (GA) for optimizing project schedule. The method has four main modules that support two optimization methods: modeling uncertainty and defuzzification module; scheduling module; cost calculations module; and decision-support module. The first optimization method uses the elitist non-dominated sorting genetic algorithm (NSGA-II), while the second uses a dynamic weighted optimization genetic algorithm. The developed scheduling and optimization methods are coded in python as a stand-alone automated computerized tool to facilitate the developed method's application.FindingsThe developed method is applied to a numerical example to demonstrate its use and illustrate its capabilities. The method was validated using a multi-layered comparative analysis that involves performance evaluation, statistical comparisons and stability evaluation. Results indicated that NSGA-II outperformed the weighted optimization method, resulting in a better global optimum solution, which avoided local minima entrapment. Moreover, the developed method was constructed under a deterministic scenario to evaluate its performance in finding optimal solutions against the previously developed literature methods. Results showed the developed method's superiority in finding a better optimal set of solutions in a reasonable processing time.Originality/valueThe novelty of the proposed method lies in its capacity to consider resource planning and project scheduling under uncertainty simultaneously while accounting for activity splitting.
Developing a measurement of employee learning agility
Authors: Lee Jinju , Song Ji Hoon 
Published: June 2021

Purpose
The purpose of this study is to develop a reliable and valid measurement of employee learning agility for use in human resources development.

Design/methodology/approach
This paper analyzed a total of 365 cases collected from Korean organizations. This paper conducted exploratory factor analysis, confirmatory factor analysis and Rasch analysis to validate the measurement. This paper conducted a measurement invariance (MI) test to confirm the generalizability of the measurement and used Harman’s single factor test to assess the common method variance (CMV).

Findings
This paper derived a learning agility measurement with six subfactors (Cronbach’s α = 0.893). This paper verified that it could be applied equally to supervisors and subordinates using an MI test. Factor analysis confirmed the feasibility of CMV. Based on I-ADAPT theory, the learning agility measurement can be applied to assess not only leader competency but also general employee competency.

Research limitations/implications
Practitioners may use this model of learning agility for developing competency-based training programs and evaluations. This study is meaningful because it extends the concept of learning agility. In particular, the MI test indicated that there are no differences between supervisors and employees regarding the utility of the concept.

Originality/value
This study is meaningful because it extends the concept of learning agility. In particular, the MI test indicated that there are no differences between supervisors and employees regarding the utility of the concept. The concept in this paper is distinguished from other studies by applying the Rasch model from an item response theory perspective.
Natural convection in a horizontal annular sector containing heat-generating porous medium
Authors: Machado dos Santos Beatriz , de Sá Ludimila Silva Salles , Su Jian 
Published: June 2021

Purpose
The purpose of this work is to propose the generalized integral transform technique (GITT) for the investigation of two-dimensional steady-state natural convection in a horizontal annular sector containing heat-generating porous medium.

Design/methodology/approach
GITT was used to investigate steady-state natural convection in a horizontal annular sector containing heat-generating porous medium. The governing equations in stream function formulation are integral transformed in the azimuthal direction, with the resulting system of nonlinear ordinary differential equations numerically solved by finite difference method. The GITT solutions are validated by comparison with fully numerical solutions by finite difference method, showing excellent agreement and convergence with low computational cost.

Findings
The effects of increasing Rayleigh number are more noticeable in stream function, whereas less significant for temperature. With decreasing annular sector angle from π to π/6, a reduction in the maximum temperature and stream function was noticed. While the two counter-rotating vortical structure is common for all annular sector angles investigated, the relative size of the two vortices varies with decreasing sector angle, with the vortex near the outer radius of the cavity becoming dominant. The annular sector angle affects strongly the maximum temperature and the partition of heat transfer on the inner and outer surfaces of the annular sector with heat-generating porous medium.

Originality/value
The strong effects of the annular sector angle on natural convection in annular sectors containing heat-generating porous medium are investigated for the first time. The proposed hybrid analytical–numerical approach can be applied in other convection problems in cylindrical or annular configurations, with or without porous medium. It shows potential for applications in practical convection problems in the nuclear and other industries.
Millennial social media users' intention to travel: the moderating role of social media influencer following behavior
Authors: Han Joyce , Chen Han 
Published: June 2021

PurposeSocial media (SoMe) influencer marketing is a popular practice. The current study examines the interplays between SoMe influencers' source credibility, Millennial users' attitudes and intention to travel. It further investigates the moderating role of SoMe influencer following behavior on the aforementioned relationships.Design/methodology/approachA total of 212 useable responses were collected through an online survey. Structural equation modeling and hierarchical multiple regressions were employed for hypotheses testing.FindingsResults indicated that source credibility had a significantly positive influence on the SoMe users' attitudes, which in turn was positively associated with the intention to visit the endorsed destination. Moreover, both relationships were strengthened for SoMe influencer followers than for nonfollowers.Originality/valueThe study expanded the source credibility theory to the use of SoMe influencer marketing on travel destinations among Millennial SoMe users. In addition, the research applied the self-determination theory to fill the gap in literature by examining the moderating role of SoMe influencer following behavior.
Unraveling the complex nexus of punitive supervision and deviant work behaviors: findings and implications from hospitality employees in Pakistan
Authors: Sarwar Aisha , Muhammad Lakhi , Sigala Marianna 
Published: June 2021

PurposeThe study adopts the conservation of resources (COR) theory for providing a better theoretical understanding of punitive supervision as an antecedent of employees’ minor deviant behaviors (namely, employee time theft and knowledge hiding) via creating cognitive mechanisms (employees’ perceived incivility). The purpose of this paper is to examine the moderating role of employees’ RESILIENCY on employees’ ability to buffer the impacts of punitive supervision.Design/methodology/approachData was gathered from 265 frontline hospitality employees in Pakistan. A survey was administered in person to establish trust and rapport with employees and so, collect reliable data.FindingsThe findings confirmed a direct and mediated impact of punitive supervision on employee minor deviant behaviors via creating perceived incivility. The moderating role of employees’ resiliency was also confirmed, as the employees’ resiliency helped them mitigate the impact of punitive supervision on perceived incivility.Research limitations/implicationsData was collected from employees’ perceptions working in one industry and cultural setting. As employees’ perceptions (influenced by their cultural background) significantly affect their interpretations and reactions to punitive behavior, future research should validate and refine the findings by collecting data from a wider and diversified cultural and industry setting.Practical implicationsThe findings provide theoretical explanatory power of the drivers and the contextual factors leading to minor employee deviant behaviors. The findings guide managers on how to develop pro-active and re-active strategies for deterring the occurrence and eliminating the consequences of punitive supervision.Originality/valueThis study contributes to the literature in multiple ways. It identifies and validates punitive supervision as an antecedent of Deviant Work Behavior (DWB). It provides a theoretical underpinning for explaining how punitive supervision spurs cognitive mechanisms, which in turn drive DWB. It also studies the nexus between destructive supervision and its outcomes in its entirety by studying the mediated and the moderating impacts of punitive supervision and perceived incivility, respectively.
Country-of-origin cues: effects of country-related affect and product association on consumer responses to hospitality firms
Authors: Li Dongmei , Xie Lishan 
Published: June 2021

Purpose
This paper aims to investigate the impacts of country-of-origin (COO) cues, country-related affect (CRA) and country-related product associations (CRPA) on consumers’ intention to purchase hotel services.

Design/methodology/approach
A survey of 305 respondents was conducted. Mediation and conditional process tests were performed to examine the proposed theoretical framework.

Findings
The results suggest that CRA is positively correlated with consumer trust, which, in turn, affects purchase intention. For consumers with a high (vs low) level of consumer ethnocentrism (CE), the effect of CRA on trust is weaker. CRPA is positively correlated with both consumer trust and price perception, which, in turn, affect purchase intention. For consumers with rich subjective knowledge (SK) of hotel services, the effect of CRPA on price perception is weaker. The effects of COO stereotypes and the moderation effects of CE and SK hold after controlling for consumers’ age, gender, income, education and objective knowledge.

Practical implications
Hospitality practitioners can make use of different types of country-related information to communicate effectively with consumers in a global environment.

Originality/value
This research is the first to discover the different mechanisms underlying different types of COO and the boundary conditions on these effects.
A framework proposal for digital interventions in perinatal education: the result of a content analysis to WHO intra-partum care recommendations
Authors: Leite Carla V. , Almeida Ana Margarida 
Published: June 2021

Purpose
This research proposes a framework to guide the development and analysis of digital interventions, namely, through mobile applications, regarding labor and birth. By complying with current scientific evidence, this paper aims to contribute to the safeness and completeness of perinatal health education targeting expectant parents.

Design/methodology/approach
A content analysis was conducted on a document containing World Health Organization guidelines for intra-partum, considering the following categories: timeframe, care options, category of recommendation, to create a data set clearly distinguishing between recommendations and non-recommendations. Context-specific and research-context recommendations, details from dosages, measurements and timings, infant care and non-immediate postpartum topics were considered out of the scope of this study.

Findings
The results were summarized in a table, ready to be used as a data set, including the following 16 care options ranging from health, well-being and/or rights: respect, communication, companionship, pregnant person’s monitoring, status, fetal monitoring, pain relief, pain management, amenities, labor delay prevention, progress, freedom of choice, facilitation of birth, prevention of postpartum hemorrhage, umbilical cord care and recovery. These were distributed across six timeframes: always, admission, first, second and third stage of labor and immediate postpartum. In addition, recommendations and non-recommendations are displayed in different columns.

Originality/value
This transdisciplinary research intends to contribute to: future research on perinatal education; the creation of digital interventions, namely, m-health ones, targeting expectant parents by providing a framework of content coverage; the endorsement of the rights to Information and to decision-making. Ultimately, when put into practice, the framework can impact self-care through access to perinatal education.
Top management commitment and lean team members’ prosocial voice behaviour
Authors: Latif Mohammad Abdul , Vang Jan 
Published: June 2021

PurposeTop management commitment (TMC) and prosocial voice behaviour in Lean teams are vital for the successful Lean implementation. This study aims to investigate how TMC influences Lean team members’ prosocial voice behaviour and how such changed voice behaviour affects the outcome of Lean implementations.Design/methodology/approachThe authors have used a qualitative research methodology to examine six dimensions of TMC (communication, involvement, support, empowerment, encouragement and monitoring) in two ready-made garment (RMG) factories in Bangladesh. Operational performance was measured by efficiency, quality, value stream mapping, single-minute exchange dies and 5S scores. Occupational Health and Safety (OHS) was assessed by acceptable head and back positions, machine safety, use of masks and housekeeping.FindingsThe findings reveal that TMC influences Lean team members' voice behaviour positively and, thereby, company's performance. Six dimensions of TMC are all critical for mobilizing prosocial voice, which then improves productivity, OHS and enhancing employee capacity and job satisfaction.Research limitations/implicationsThis research involved two sewing lines in two RMG factories in Bangladesh. Cross-sector and large-scale international quantitative research is also needed.Practical implicationsThis research shows how TMC and Lean problem-solving teams can mobilize employee voice.Originality/valueEmployee voice is a central issue in the implementation of Lean. To the best of the author’s knowledge, for the first time, the authors show how the six dimensions of TMC influence Lean team members’ voice behaviour in the workplace and thereby how prosocial voice affects team performance.
Founder CEOs, business groups and firm value: evidence from an emerging market
Authors: Kim Woo Sung , Kiymaz Halil 
Published: June 2021

PurposeThe impact of founder CEOs on firm value continues to be debated in the finance literature. While earlier studies suggest that founding family ownership and founding CEO structure create less value than public ownership, later studies provide contradicting evidence. This study examines how founder CEOs affect firm value in the business group context while controlling for firm-specific variables and various CEO characteristics.Design/methodology/approachThe authors use a sample of publicly listed Indian firms from 2010 to 2015 with 997 firm-year data observations. While 306 of these are in business groups, the remaining 691 are in a nonbusiness group. The authors also divide the sample into various sector subgroups, including materials (170), industrials (198), consumer (422) and others (198). They use two different models, including the fixed effect model (FEM) and pooled generalized method of moments (GMM) model to run regressions.FindingsThe authors find that firms with founder CEOs have lower firm value than those with nonfounder CEOs. These results show the importance of the role of founder CEOs in the Indian business groups. The authors further find a positive relationship between founder CEO and business group interaction variable, showing that an increase in founder CEO (or business group) increases the significance effect of business group (founder CEO) on firm performance. After separating the sample business and nonbusiness groups, the relationship between founder CEOs and firm value in both groups remains negative. Using various firm-specific control variables, the authors find that highly leveraged and smaller firms experience lower Tobin's Q. In contrast, firms with more investment in research and development perform better. Among CEO characteristics, the authors find that firms with highly educated CEOs do not perform well, while firms with older CEOs do better. Finally, they find that CEO tenure and duality are associated with lower firm performance.Originality/valueThis study adds value by providing evidence on the founder CEOs and firm performance in business groups from a fast-developing emerging market.