bluuebunny/crossref_metadata_2025_split huggingface dataset:
Dislocation Creep of Olivine: Backstress Evolution Controls Transient Creep at High Temperatures
Authors: Hansen Lars N. , Wallis David , Breithaupt Thomas , Thom Christopher A. , Kempton Imogen 
Published: April 2021

AbstractTransient creep occurs during geodynamic processes that impose stress changes on rocks at high temperatures. The transient is manifested as evolution in the viscosity of the rocks until steady‐state flow is achieved. Although several phenomenological models of transient creep in rocks have been proposed, the dominant microphysical processes that control such behavior remain poorly constrained. To identify the intragranular processes that contribute to transient creep of olivine, we performed stress‐reduction tests on single crystals of olivine at temperatures of 1,250°C–1,300°C. In these experiments, samples undergo time‐dependent reverse strain after the stress reduction. The magnitude of reverse strain is ∼10−3 and increases with increasing magnitude of the stress reduction. High‐angular resolution electron backscatter diffraction analyses of deformed material reveal lattice curvature and heterogeneous stresses associated with the dominant slip system. The mechanical and microstructural data are consistent with transient creep of the single crystals arising from accumulation and release of backstresses among dislocations. These results allow the dislocation‐glide component of creep at high temperatures to be isolated, and we use these data to calibrate a flow law for olivine to describe the glide component of creep over a wide temperature range. We argue that this flow law can be used to estimate both transient creep and steady‐state viscosities of olivine, with the transient evolution controlled by the evolution of the backstress. This model is able to predict variability in the style of transient (normal vs. inverse) and the load‐relaxation response observed in previous work.
The Relationship Between Large Scale Thermospheric Density Enhancements and the Spatial Distribution of Poynting Flux
Authors: Billett D. D. , Perry G. W. , Clausen L. B. N. , Archer W. E. , McWilliams K. A. , Haaland S. , Reistad J. P. , Burchill J. K. , Patrick M. R. , Humberset B. K. , Anderson B. J. 
Published: April 2021

AbstractLarge thermospheric neutral density enhancements in the cusp region have been examined for many years. The Challenging Minisatellite Payload (CHAMP) satellite for example has enabled many observations of the perturbation, showing that it is mesoscale in size and exists statistically over solar cycle timescales. Further studies examining the relationship with magnetospheric energy input have shown that fine‐scale Poynting fluxes are associated with the density perturbations on a case‐by‐case basis, whilst others have found that mesoscale downward fluxes also exist in the cusp region statistically. In this study, we use nearly 8 years of the overlapping Super Dual Auroral Radar Network and Active Magnetosphere and Planetary Electrodynamics Response Experiment datasets to generate global‐scale patterns of the high‐latitude and height‐integrated Poynting flux into the ionosphere, with a time resolution of 2 min. From these, average patterns are generated based on the interplanetary magnetic field orientation. We show the cusp is indeed an important feature in the Poynting flux maps, but the magnitude does not correlate well with statistical neutral mass density perturbations observed by the CHAMP satellite on similar spatial scales. Importantly, the lack of correlation between mesoscale height‐integrated Poynting fluxes and the cusp neutral mass density enhancement gives possible insight into other processes that may account for the discrepancy, such as energy deposition at finer scale sizes or at higher altitudes than captured.
Deep-sea shipwrecks represent island-like ecosystems for marine microbiomes
Authors: Hamdan Leila J , Hampel Justyna J , Moseley Rachel D , Mugge Rachel L , Ray Anirban , Salerno Jennifer L , Damour Melanie 
Published: April 2021

Abstract
Biogeography of macro- and micro-organisms in the deep sea is, in part, shaped by naturally occurring heterogeneous habitat features of geological and biological origin such as seeps, vents, seamounts, whale and wood-falls. Artificial features including shipwrecks and energy infrastructure shape the biogeographic patterns of macro-organisms; how they influence microorganisms is unclear. Shipwrecks may function as islands of biodiversity for microbiomes, creating a patchwork of habitats with influence radiating out into the seabed. Here we show microbiome richness and diversity increase as a function of proximity to the historic deep-sea shipwreck Anona in the Gulf of Mexico. Diversity and richness extinction plots provide evidence of an island effect on microbiomes. A halo of core taxa on the seabed was observed up to 200 m away from the wreck indicative of the transition zone from shipwreck habitat to the surrounding environment. Transition zones around natural habitat features are often small in area compared to what was observed at Anona indicating shipwrecks may exert a large sphere of influence on seabed microbiomes. Historic shipwrecks are abundant, isolated habitats with global distribution, providing a means to explore contemporary processes shaping biogeography on the seafloor. This work is a case study for how built environments impact microbial biodiversity and provides new information on how arrival of material to the seafloor shapes benthic microbiomes.
Discovery of electrochemically induced grain boundary transitions
Authors: Nie Jiuyuan , Hu Chongze , Yan Qizhang , Luo Jian 
Published: April 2021

AbstractElectric fields and currents, which are used in innovative materials processing and electrochemical energy conversion, can often alter microstructures in unexpected ways. However, little is known about the underlying mechanisms. Using ZnO-Bi2O3 as a model system, this study uncovers how an applied electric current can change the microstructural evolution through an electrochemically induced grain boundary transition. By combining aberration-corrected electron microscopy, photoluminescence spectroscopy, first-principles calculations, a generalizable thermodynamic model, and ab initio molecular dynamics, this study reveals that electrochemical reduction can cause a grain boundary disorder-to-order transition to markedly increase grain boundary diffusivities and mobilities. Consequently, abruptly enhanced or abnormal grain growth takes place. These findings advance our fundamental knowledge of grain boundary complexion (phase-like) transitions and electric field effects on microstructural stability and evolution, with broad scientific and technological impacts. A new method to tailor the grain boundary structures and properties, as well as the microstructures, electrochemically can also be envisioned.
Quantitative imaging of intracellular nanoparticle exposure enables prediction of nanotherapeutic efficacy
Authors: Yin Qingqing , Pan Anni , Chen Binlong , Wang Zenghui , Tang Mingmei , Yan Yue , Wang Yaoqi , Xia Heming , Chen Wei , Du Hongliang , Chen Meifang , Fu Chuanxun , Wang Yanni , Yuan Xia , Lu Zhihao , Zhang Qiang , Wang Yiguang 
Published: April 2021

AbstractNanoparticle internalisation is crucial for the precise delivery of drug/genes to its intracellular targets. Conventional quantification strategies can provide the overall profiling of nanoparticle biodistribution, but fail to unambiguously differentiate the intracellularly bioavailable particles from those in tumour intravascular and extracellular microenvironment. Herein, we develop a binary ratiometric nanoreporter (BiRN) that can specifically convert subtle pH variations involved in the endocytic events into digitised signal output, enabling the accurately quantifying of cellular internalisation without introducing extracellular contributions. Using BiRN technology, we find only 10.7–28.2% of accumulated nanoparticles are internalised into intracellular compartments with high heterogeneity within and between different tumour types. We demonstrate the therapeutic responses of nanomedicines are successfully predicted based on intracellular nanoparticle exposure rather than the overall accumulation in tumour mass. This nonlinear optical nanotechnology offers a valuable imaging tool to evaluate the tumour targeting of new nanomedicines and stratify patients for personalised cancer therapy.
Structural variation at the maize WUSCHEL1 locus alters stem cell organization in inflorescences
Authors: Chen Zongliang , Li Wei , Gaines Craig , Buck Amy , Galli Mary , Gallavotti Andrea 
Published: April 2021

AbstractStructural variation in plant genomes is a significant driver of phenotypic variability in traits important for the domestication and productivity of crop species. Among these are traits that depend on functional meristems, populations of stem cells maintained by the CLAVATA-WUSCHEL (CLV-WUS) negative feedback-loop that controls the expression of the WUS homeobox transcription factor. WUS function and impact on maize development and yield remain largely unexplored. Here we show that the maize dominant Barren inflorescence3 (Bif3) mutant harbors a tandem duplicated copy of the ZmWUS1 gene, ZmWUS1-B, whose novel promoter enhances transcription in a ring-like pattern. Overexpression of ZmWUS1-B is due to multimerized binding sites for type-B RESPONSE REGULATORs (RRs), key transcription factors in cytokinin signaling. Hypersensitivity to cytokinin causes stem cell overproliferation and major rearrangements of Bif3 inflorescence meristems, leading to the formation of ball-shaped ears and severely affecting productivity. These findings establish ZmWUS1 as an essential meristem size regulator in maize and highlight the striking effect of cis-regulatory variation on a key developmental program.
Mechanism and toxicity evaluation of catalytic ozonation over Cu/Ce–Al2O3 system aiming at degradation of humic acid in real wastewater
Authors: Tang Xi , Zhang Yifei , Li Weiqi , Geng Jinju , Ren Hongqiang , Xu Ke 
Published: April 2021

AbstractHumic acid (HA) is the main component of organic matter in effluent from wastewater treatment. The effective removal of HA is significant. In this study, a novel catalyst was prepared using a transition metal oxide as the active component and Al2O3as a granular carrier. The mechanism of catalytic ozonation of HA under neutral pH conditions and its efficiency were investigated. Under the chosen conditions (an ozone concentration of 2.2 mg/L, 50 mg/L HA solution, catalyst dosage of 5 g/L and initial pH of 6.49), the Cu/Ce–Al2O3bimetallic catalyst led to 54.79% TOC removal rate after 30 min; the removal rate by ozone alone was only 20.49%. The characteristics of organic compounds determined by FT-IR and GC–MS showed that organic compounds were degraded significantly by the catalytic treatment. The addition of catalysts could effectively degrade toxic intermediates and reduce the acute toxicity produced by ozonation. Humic acid substances were largely removed and transformed into biodegradable intermediates. This study proposes a new and efficient ozonation catalyst for practical applications in advanced wastewater treatment.
Clinicopathological factors associated with synchronous distant metastasis and prognosis of stage T1 colorectal cancer patients
Authors: Li Qiken , Wang Gang , Luo Jun , Li Bo , Chen Weiping 
Published: April 2021

AbstractIt is rare and understudied for patients with stage T1 colorectal cancer to have synchronous distant metastasis. This study was to determine the clinicopathological factors associated with distant metastasis and prognosis. T1 colorectal cancer patients diagnosed between 2010 and 2015 were obtained from the SEER database. Logistic regression was applied to determine risk factors related to distant metastasis. Cox-proportional hazard models were used to identify the prognostic factors for patients with distant metastasis. Among 21,321 patients identified, 359 (1.8%) had synchronous distant metastasis and 1807 (8.5%) had lymph node metastasis. Multivariate analysis revealed that younger age, positive serum CEA, larger tumor size, positive tumor deposit, perineural invasion, lymph node metastasis, histology of non-adenocarcinoma and poorer differentiation were significantly associated with the increased risk of synchronous distant metastasis. Older age, female, Black, positive CEA, positive lymph node metastasis, positive tumor deposit, larger tumor size, no chemotherapy, inadequate lymph node harvesting and no metastasectomy were correlated with worse survival in these patients with synchronous distant metastasis. Patients with metastasis to the liver displayed the highest rate of positive CEA. We conclude that T1 colorectal cancer patients with multiple risk factors need thorough examinations to exclude synchronous distant metastasis. Chemotherapy, adequate lymph node cleaning and metastasectomy are associated with improved survival for those patients with distant metastases. Positive serum CEA may be useful in predicting distant metastases in patients at stage T1.
Fabrication and characterization of keratin starch biocomposite film from chicken feather waste and ginger starch
Authors: Oluba Olarewaju M. , Obi Chibugo F. , Akpor Oghenerobor B. , Ojeaburu Samuel I. , Ogunrotimi Feyikemi D. , Adediran Adeolu A. , Oki Makanjuola 
Published: April 2021

AbstractThe disposal of chicken feather through burning or burying is not environmentally compliant due to the accompanying release of greenhouse gas and underground water contamination. Thus, the transformation of this bio-waste into a bio-composite film is considered not only a sustainable strategy for disposal of this solid wastes but also an attractive alternative to developing an efficient nanostructured biomaterial from renewable bio resource. In the present study keratin extracted from chicken feather waste in combination with ginger starch were fabricated into a bio-composite film. The fabricated bio-composite films were characterized, using different analytical techniques. The physicochemical characteristics of ginger starch showed a moisture content of 33.8%, pH of 6.21, amylose and amylopectin contents of 39.1% and 60.9%, respectively. The hydration capacity of the starch was 132.2% while its gelatinization temperature was 65.7 °C. Physical attributes of the bio-composite film, such as surface smoothness and tensile strength increased significantly (p < 0.05) with increasing keratin content, while its transparency and solubility showed significant (p < 0.05) decrease with increasing keratin level. The various blends of the bio-composite films decayed by over 50% of the original mass after 12 days of complete burial in soil. Based on the results obtained in this study, the addition of keratin to starch bio-composite showed remarkable improvement in mechanical properties, such as tensile strength and surface smoothness. The bio-composite film exhibited appropriate stability in water, although future study should be carried out to evaluate its thermal stability. Nonetheless, the fabricated keratin-starch bio-composite showed desirable characteristics that could be optimized for industrial applications.
Scrt1, a transcriptional regulator of β-cell proliferation identified by differential chromatin accessibility during islet maturation
Authors: Sobel Jonathan , Guay Claudiane , Elhanani Ofer , Rodriguez-Trejo Adriana , Stoll Lisa , Menoud Véronique , Jacovetti Cécile , Walker Michael D. , Regazzi Romano 
Published: April 2021

AbstractGlucose-induced insulin secretion, a hallmark of mature β-cells, is achieved after birth and is preceded by a phase of intense proliferation. These events occurring in the neonatal period are decisive for establishing an appropriate functional β-cell mass that provides the required insulin throughout life. However, key regulators of gene expression involved in functional maturation of β-cells remain to be elucidated. Here, we addressed this issue by mapping open chromatin regions in newborn versus adult rat islets using the ATAC-seq assay. We obtained a genome-wide picture of chromatin accessible sites (~ 100,000) among which 20% were differentially accessible during maturation. An enrichment analysis of transcription factor binding sites identified a group of transcription factors that could explain these changes. Among them, Scrt1 was found to act as a transcriptional repressor and to control β-cell proliferation. Interestingly, Scrt1 expression was controlled by the transcriptional repressor RE-1 silencing transcription factor (REST) and was increased in an in vitro reprogramming system of pancreatic exocrine cells to β-like cells. Overall, this study led to the identification of several known and unforeseen key transcriptional events occurring during β-cell maturation. These findings will help defining new strategies to induce the functional maturation of surrogate insulin-producing cells.
Consumption of caffeinated beverages and kidney function decline in an elderly Mediterranean population with metabolic syndrome
Authors: Díaz-López Andrés , Paz-Graniel Indira , Ruiz Verónica , Toledo Estefanía , Becerra-Tomás Nerea , Corella Dolores , Castañer Olga , Martínez J. Alfredo , Alonso-Gómez Ángel M. , Wärnberg Julia , Vioque Jesús , Romaguera Dora , López-Miranda José , Estruch Ramon , Tinahones Francisco J. , Lapetra José , Serra-Majem Luís , Bueno-Cavanillas Aurora , Tur Josep A. , Sánchez Vicente Martín , Pintó Xavier , Delgado-Rodríguez Miguel , Matía-Martín Pilar , Vidal Josep , Vázquez Clotilde , Daimiel Lidia , Villa Tania Fernandez , Ros Emilio , Eguaras Sonia , Babio Nancy , Sorlí Jose V. , Goday Albert , Abete Itziar , Sierra Lucas Tojal , Barón-López Francisco Javier , Torres-Collado Laura , Morey Marga , Garcia-Rios Antonio , Casas Rosa , Bernal-López María Rosa , Santos-Lozano José Manuel , Navarro Adela , Gonzalez Jose I. , Zomeño María Dolores , Zulet Maria Angeles , Luna Jessica Vaquero , Ramallal Raul , Fitó Montse , Salas-Salvadó Jordi 
Published: April 2021

AbstractIt remains unclear whether caffeinated beverages could have deleterious renal effects in elderly population with underlying comorbid conditions. We investigated the associations between coffee, tea, or caffeine intake and 1-year changes in glomerular filtration rate (eGFR) in a large Spanish cohort of overweight/obese elderly with metabolic syndrome (MetS). This prospective analysis includes 5851 overweight/obese adults (55–75 years) with MetS from the PREDIMED-Plus study. We assessed coffee, tea, and caffeine consumption from a validated food-frequency questionnaire and creatinine-based eGFR using the Chronic Kidney Disease Epidemiology Collaboration equation. Multivariate-adjusted regression models were applied to test associations between baseline coffee, tea, or caffeine intake and 1-year eGFR changes. Caffeinated coffee (> 2 cups/day) and tea (at least 1 cup/day) drinkers had 0.88 and 0.93 mL/min/1.73 m2 greater eGFR decrease respectively, compared to those with less than 1 cup/day of coffee consumption or non-tea drinkers. Furthermore, caffeinated coffee consumption of > 2 cups/day was associated with 1.19-fold increased risk of rapid eGFR decline > 3 mL/min/1.73 m2 (95% CI 1.01–1.41). Similarly, individuals in the highest (median, 51.2 mg/day) tertile of caffeine intake had a 0.87 mL/min/1.73 m2 greater eGFR decrease. Decaffeinated coffee was not associated with eGFR changes. In conclusion, higher consumption of caffeinated coffee, tea, and caffeine was associated with a greater 1-year eGFR decline in overweight/obese adults with MetS.
Projected effects of ocean warming on an iconic pelagic fish and its fishery
Authors: Moltó Vicenç , Palmer Miquel , Ospina-Álvarez Andrés , Pérez-Mayol Sílvia , Benseddik Amina Besbes , Gatt Mark , Morales-Nin Beatriz , Alemany Francisco , Catalán Ignacio A. 
Published: April 2021

AbstractIncreasing sea temperature is a driver of change for many fish traits, particularly for fast-growing epipelagic species with short life spans. With warming, altered spawning phenology and faster growth may produce substantially larger body sizes of the new cohort, affecting fishery productivity. We present an individual-based model (IBM) that predicts the distribution of fish length at catch under observed and projected thermal scenarios, accounting for mortality, temperature-dependent spawning phenology, temperature- and photoperiod- dependent growth. This IBM was demonstrated with Coryphaena hippurus (common dolphinfish), a circumglobally-distributed and highly thermophilic species sustaining commercial and recreational fisheries where it is present. The model projected a 13.2% increase in the average length at catch under marine heatwave conditions compared to the current thermal regime (1995–2005 average). Projections under RCP scenarios 4.5 and 8.5 by the end of the century led to 5.1% and 12.8% increase in average length, respectively. Furthermore, these thermal scenarios affected spawning phenology differently, producing higher variance in body size under RCP 8.5 scenario with respect to marine heatwave conditions. This study highlights how the environmental effects of climate change can alter the distribution of species length at catch.
Identification of transcriptional subtypes in lung adenocarcinoma and squamous cell carcinoma through integrative analysis of microarray and RNA sequencing data
Authors: Fauteux François , Surendra Anuradha , McComb Scott , Pan Youlian , Hill Jennifer J. 
Published: April 2021

AbstractClassification of tumors into subtypes can inform personalized approaches to treatment including the choice of targeted therapies. The two most common lung cancer histological subtypes, lung adenocarcinoma and lung squamous cell carcinoma, have been previously divided into transcriptional subtypes using microarray data, and corresponding signatures were subsequently used to classify RNA-seq data. Cross-platform unsupervised classification facilitates the identification of robust transcriptional subtypes by combining vast amounts of publicly available microarray and RNA-seq data. However, cross-platform classification is challenging because of intrinsic differences in data generated using the two gene expression profiling technologies. In this report, we show that robust gene expression subtypes can be identified in integrated data representing over 3500 normal and tumor lung samples profiled using two widely used platforms, Affymetrix HG-U133 Plus 2.0 Array and Illumina HiSeq RNA sequencing. We tested and analyzed consensus clustering for 384 combinations of data processing methods. The agreement between subtypes identified in single-platform and cross-platform normalized data was then evaluated using a variety of statistics. Results show that unsupervised learning can be achieved with combined microarray and RNA-seq data using selected preprocessing, cross-platform normalization, and unsupervised feature selection methods. Our analysis confirmed three lung adenocarcinoma transcriptional subtypes, but only two consistent subtypes in squamous cell carcinoma, as opposed to four subtypes previously identified. Further analysis showed that tumor subtypes were associated with distinct patterns of genomic alterations in genes coding for therapeutic targets. Importantly, by integrating quantitative proteomics data, we were able to identify tumor subtype biomarkers that effectively classify samples on the basis of both gene and protein expression. This study provides the basis for further integrative data analysis across gene and protein expression profiling platforms.