bluuebunny/crossref_metadata_2025_split huggingface dataset:
“Part of Getting to Where We Are is Because We Have Been Open to Change”Integrating Community Health Workers on Care Teams at Ten Ryan White HIV/AIDS Program Recipient Sites
Authors: Martinez Linda Sprague , Davoust Melissa , Rajabiun Serena , Baughman Allyson , Bachman Sara , Bowers-Sword Rachel , Rojo Maria Campos , Sullivan Marena , Drainoni Mari-Lynn 
Published: January 2021

Abstract
Background: Community Health Workers (CHWs) have long been integrated in the delivery of HIV care, in middle- and low-income countries. However, less is known about CHW integration into HIV care teams in the United States (US). To date, US based CHW integration studies have studies explored integration in the context of primary care and patient-centered medical homes.There is a need for research related to strategies that promote the successful integration of CHWs into HIV care delivery systems. In 2016, the Health Resources and Services Administration HIV/AIDS Bureau launched a three-year initiative to provide training, technical assistance and evaluation for Ryan White HIV/AIDS Program (RWHAP) recipient sites to integrate CHWs into their multidisciplinary care teams, and in turn strengthen their capacity to reach communities of color and reduce HIV inequities. Methods: Ten RWHAP sites were selected from across eight states. The multi-site program evaluation included a process evaluation guided by RE-AIM to understand how the organizations integrated CHWs into their care teams. Site team members participated in group interviews to walk-the-process during early implementation and following the program period. Directed content analysis was employed to examine program implementation. Codes developed using implementation strategies outlined in the Expert Recommendations for Implementing Change project were applied to group interviews (n=20). Findings: Implementation strategies most frequently described by sites were associated with organizational-level adaptations in order to integrate the CHW into the HIV care team. These included revising, defining, and differentiating professional roles and changing organizational policies. Strategies used for implementation, such as network weaving, supervision, and promoting adaptability, were second most commonly cited strategies, followed by training and TA strategies. Conclusions: Wrapped up in the implementation experience of the sites there were some underlying issues that pose challenges for health care organizations. Organizational policies and the ability to adapt proved significant in facilitating CHW implementation. The integration of the CHW role may present an occasion for health care delivery organizations to reassess policies that may unintentionally marginalize communities and both limit career opportunities and patient engagement.
Application of Laparoscopic Gastric Jejunum Uncut Roux-en-Y&nbsp;Anastomosis
Authors: Yu Chao , Yan Qiang , Li De-Guan , Wang Yi-Gao , Yang Xiao-Dong , Zhang Shang-Xin , Zhang Yong-Hong , Zhang Zhen 
Published: January 2021

Abstract
Background: Uncut Roux en-Y gastrojejunostomy, recently developed in China is useful in the treatment of distal gastric cancer. This study aims to compare laparoscopic gastric jejunum Uncut Roux-en-Y anastomosis with conventional anastomosis in the surgical treatment of distal gastric malignancy.Methods: A total of 178 cases diagnosed with distal gastric malignant tumors were surgically treated in the First affiliated Hospital of Anhui University of cavity mirrors. In this retrospective study, the clinical data of patients and their follow-up records were analyzed. Out of the 178 cases, 112 cases (uncut group) were the observation group for stomach jejunum Uncut Roux-en-Y anastomosis, the control group for the stomach, 66 cases (conventional group) were for jejunum Roux-en-Y anastomosis and Billroth Ⅰ and Billroth Ⅱ anastomosis. A comparison between the two groups was conducted based on the general situation of the patients, TNM stage, and one-year survival rate. A P-value of less than 0.05 was considered statistically significant (p<0.05), while a P-value of more than 0.05 was considered statistically insignificant (p>0.05).Results: There was no significant difference was reported between the two groups in terms of the general situation and TNM stage. A comparison on postoperative complications between the two groups revealed that the incidence rate of postoperative anastomotic bleeding was 0.9% (1/112), 6.1% (4/66), the incidence rate of alkaline reflux gastritis was 1.8% (2/112), 9.1% (6/66), the incidence rate of anastomotic fistula was 0.0% (0/112), 3.0% (3/66), and the Roux retention syndrome (RSS) incidence rate was 0.9% (1/112), 7.6% (5/66). The observation group significantly lower than the control group, and the difference was statistically significant. The incidence rate of overall complications was at 3.6% (4/112) and 25.8% (17/66), which was significantly lower in the observation group than in the control group, and the difference was statistically significant. Notably, there was no significant difference in 1-year survival rate between the two groups.Conclusion: Laparoscopic gastric jejunal Uncut Roux-en-Y anastomosis significantly reduces the risk of postoperative complications of the digestive tract. Its operation is easy and exhibits an effective curative effect. Moreover, it has a better surgical effect and a promising clinical application prospect compared to conventional gastric jejunal anastomosis.
Investigating The Efficiency of Photothrombosis-Associated Focal Cerebral Ischemia By Monitoring Histopathological Changes and Neurobehavioral Performance in Model of Mouse
Authors: Shabani Zahra , Farhoudi Mehdi , Rahbarghazi Reza , Karimipour Mohammad , Mehrad Hossein 
Published: January 2021

Abstract
Background: Photothrombotic (PT) stroke model is a reliable, reproducible, noninvasive and accessible method to induce ischemic stroke in the target site using excitation of photosensitive agents such as Rose Bengal (RB) dye after light illumination. Methods: The thirty animals were assigned into three groups of Laser irradiation, RB, and Laser irradiation+RB. We assessed ischemic stroke outcomes through multiple cellular and behavioral approaches. Laser irradiation+ RB promoted pale ischemic changes after 7 days. Microscopic staining revealed neural tissue degeneration and demarcated necrotic site and neuronal injury plus prominent astrogliosis in the periphery of irradiated sites. We performed (BrdU/NeuN) staining to examine the neurogenesis on day 28. The volume of the lesion site was calculated using unbiased stereological study and Cavalier’s principle. To assess the efficiency of ischemic stroke, modified neurological severity score (mNSS) and cylinder tests were done on days 1, 7, 14, and 28. Results: BrdU+/NeuN+ staining showed a significant increase in the number of proliferating cells in the Laser irradiation+ RB group compared to the other groups (p<0.05), while the percent of NeuN+ cells reduced. In Laser irradiation + RB group, we found the largest infarct cavity volume and functional deficits during post ischemic days 1-7 that reduced thereafter. Conclusion: Our study revealed that the PT although increased the cell proliferating in the periphery of the lesion site, but due to an undesirable microenvironment, the neurogenesis decreased concomitantly with the functional and infarct size alleviation.
Effects of Content Features and Lingual Form of Government Information Release on the Regulation of Public Negative Emotions During&nbsp;COVID-19 Epidemic in Wuhan
Authors: zhu yanchun , zhang wei , zhang mei , qin chunlei , wen jianbo 
Published: January 2021

Abstract
Background: Emergencies and their associated negative emotions have a great effect on public health. As a key part of the emergency management, government information release (GIR) not only meets the public's health information seeking, but also helps to eliminate the breeding and spreading of negative social emotions. Method: From the two aspects of content features and lingual forms, a regression model was built to explore the mechanism of GIR on the regulation of netizens' negative emotions by adopting the theoretical methods of content analysis, emotion calculation, and case analysis. Results: During the emergency outbreak, if the government can timely release information on the incident and respond to the public using rational language, netizens' negative emotions can be alleviated. During the emergency peak, the government should release the event progress, resolution and disposal information to improve the recognition of netizens and eliminate negative emotions. Conclusions: According to different stages of emergencies, the government should timely and reasonably utilize the attitude tendency, content type and lingual form of GIR to effectively regulate the negative emotions of netizens.
Increased Risk of Osteoporosis in Patients With Nephrotic Syndrome. A Nationwide Population-Based Retrospective Cohort Study
Authors: Liao Chen-Yi , Chung Chi-Hsiang , Wei Kuang-yu , Feng Tseng-Min , Lin Fu-Huang , Tsao Chang-Huei , Wu Chia-Chao , Chu Pauling , Chien Wu-Chien 
Published: January 2021

Abstract
Corticosteroid (CS) is commonly used in nephrotic syndrome (NS) and a risk factor for osteoporosis. We evaluated the risk of osteoporosis in patients with nephrotic syndrome using a nationwide population-based dataset. After adjusting for covariates, osteoporosis risk was found to be 3.279 times greater in the NS cohort than in the non-NS cohort, when measured over 11 years after NS diagnosis. Stratification revealed that age older than 18 years, congestive heart failure, hyperlipidemia, chronic kidney disease, liver cirrhosis, and NS-related disease including diabetes mellitus, hepatitis B infection, hepatitis C infection, lymphoma, and hypothyroidism, increased the risk of osteoporosis is when comparing the NS cohort with the non-NS cohort. Additionally, osteoporosis risk was significantly higher in NS patients with CS use (aHR = 3.397). The risk of osteoporosis in NS patients was positively associated with risk of hip and vertebral fracture (aHR = 2.130 and 2.268, respectively). A significant association exists between NS and subsequent risk for osteoporosis. Therefore NS patients, particularly those treated with CS, should be evaluated for subsequent risk of osteoporosis.
Molecular Serotyping and Resistance of Clinical Strains of Haemophilus (Glaesserella) Parasuis in Chinese Pig Farms From 2016 to 2018.
Authors: Liu Jinjin , Guo Long , Yuan Yi , Song Wenbo , Li Qianqian , Huang Ying , Long Yunzhi , Yang Liu , Liang Gong , Huang Chao , Tang Xibiao 
Published: January 2021

Abstract
Haemophilus parasuis (H. parasuis) is the etiological agent of Glässer's disease and brings great economic losses to the pig industry. The goal of our research is to reveal the serotypes of H. parasuis isolated from large-scale pig farms in China from 2016 to 2018. From 2016 to 2018, 8153 H. parasuis field strains were isolated from 14610 clinical samples of sick pigs with clinical symptoms from 26 provinces and cities of China. Among them, 1386 strains were identified as H. parasuis by PCR, and the isolation rate was 9.49%. Through multiplex PCR, we showed that type 5/12 and type 4 strains had the highest separation rate, followed by type 13 and type 14 strains. Using disk diffusion method, we found cephalosporin antibiotics and peptide antibiotics all had good inhibitory effect on H. parasuis. Our conclusion may play a positive role in the prevention and treatment of H. parasuis.
Ultrasound Elastographic Features of the Fetlock Joint Capsule in Horses
Authors: Straticò Paola , Guerri Giulia , Palozzo Adriana , Francesco Paola Di , Vignoli Massimo , Varasano Vincenzo , Petrizzi Lucio 
Published: January 2021

Abstract
Background: Capsulitis leads to the release of inflammatory mediators into the joint causing capsular fibrosis and osteoarthrosis (OA). Strain elastosonography (SE) measures the elasticity of tissue evaluating its strain to an operator-dependent deformation. Aims of the study were to assess the feasibility of SE for evaluating the distal attachment of the joint capsule (DJC) of fore fetlocks in sound horses and in horses with fetlock OA and to evaluate differences in their elastosonographic aspect. After a whole lameness examination, fore fetlocks DJC were assigned to Group S and Group P and examined by two operators using SE. The inter-rater reliability (IRR), the intraclass (intra-CC) and interclass (inter-CC) correlation coefficients were used to compare colour grading scores, repeatability, reproducibility of elasticity index (EI) and strain ratio (SR). The same parameters were compared between groups. Findings were significant for P < 0.05. Results: Forty-one horses were included, 11 in Group S, 30 in Group P (16 with bilateral OA, 8 left and 6 right OA). IRRs ranged from good to excellent. For transverse and longitudinal scans, the EIs of Group S were 0.57-0.51, SRs were 0.32-0.28. Inter-CC and intra-CC were always > 0.8. In Group P, EI was 1.09-0.89, SRs was 0.78-0.72 with intra-CC and inter-CC >0.57. Significative differences of EI and SR were detected between groups, and between Group S and the affected limb of Group P.Conclusions: SE is a useful technique for evaluating the DJC, with good repeatability and reproducibility. DJC appears softer in sound horses.
Association of Food Insecurity With Dietary Patterns and Expenditure on Food, Alcohol and Tobacco Amongst Indigenous Inuit in Greenland: Results From a Population Health Survey.
Authors: Bjerregaard Peter , Olesen Ingelise , Larsen Christina 
Published: January 2021

Abstract
Background Amongst the indigenous Greenlandic Inuit, the experience of food insecurity has been attributed to a lack of money to buy enough food of sufficient quality to sustain a family, although a preference for alcohol and tobacco over food has also been cited. The purpose of the article was to compare dietary patterns and expenditure on food, alcoholic beverages and tobacco between survey participants who reported food insecurity and those who did not.Methods Countrywide cross-sectional health survey among 1886 adult Greenlandic Inuit in 2018. Diet was estimated by a food frequency questionnaire. Food insecurity status was based on the household hunger scale. Analyses were carried out by univariate general linear models adjusted for age, sex and social position. ResultsNine percent of the participants reported food insecurity. Food insecurity was unevenly distributed according to age, sex and social position. Food insecure participants more often chose an unhealthy dietary pattern (43% vs. 32%) and they reported a higher energy intake. The food insecure spent the same amount of money on food as other participants but less on nutritious food and more on non-nutritious food. The cost per kJ of the food of the food insecure was lower than that of the food secure (DKK 8.0 and 9.0 per 1000 kJ, respectively). The food insecure participants also spent considerably more on alcohol and tobacco. Conclusions The results suggest that it is not only unemployment and lack of money that creates food insecurity and unhealthy dietary patterns in Greenland but probably also social inequality, lack of resources for planning, prioritising, living one’s daily life and limited knowledge about food. There seems to be at least two population subgroups in Greenland with poverty and substance use, respectively, as the immediate determinants for food insecurity, but behind these causes is a web of underlying social causes. The results are important for the design of interventions against food insecurity and unhealthy dietary patterns.
A germline variant of human IgG1+ B cell receptor represses colorectal tumorigenesis and progression by shaping the tumor microenvironment
Authors: Liu Wanli , Yang Bing , Zhang Zheng , Chen Xiangjun , Wang Xu-Yan , Qin Shishang , Du Liaoqi , Yang Changjiang , Sun Wenbo , Zhu Liyu , Zheng Qinwen , Zhu Yongjie , Song Xiya , Zhao Shidong , Wang Quan , Zhao Long , Lin Yilin , Yan Xiaohan , Gao Ji , Huang Jinghe , Wu Fan , Lu Lu , Wang Fei , Zheng Wenjie , Zhou Xiao-Hua , Zhao Xiaozhen , Wang Ziye , Sun Xiaolin , Ye Yingjiang , Wang Shan , Li Zhan-Guo , Qi Hai , Zhang Zemin , Kuang Dong-Ming , Zhang Lei , Shen Zhanlong 
Published: January 2021

Abstract
The presence of B lymphocytes in tumor tertiary lymphoid structures (TLSs) is an important prognostic indicator for different types of cancers. However, whether B cell responses in the tumor microenvironment (TME) can be harnessed for immunotherapy is unclear. Here we report that a protective germline variant of human immunoglobulin heavy constant gamma 1 gene (IGHG1) containing a Gly396 to Arg396 substitution (hIgG1-G396R) confers improved survival of colorectal cancer (CRC) patients. These hIgG1-G396R homozygous CRC patients displayed elevated tumor-associated antigen (TAA)-specific IgG1 antibody production and plasma cell infiltration into tumors. In murine colon carcinoma models, mice expressing the murine functional homolog IgG2c-Gly400Arg variant (mIgG2c-G400R) also produce higher levels of tumor-specific IgG2c antibodies via enhanced plasma cell differentiation, together with alleviated tumorigenesis and progression. Mechanistically, this variant potentiates TAA-specific antibody-dependent cellular phagocytosis and antigen presentation. Comprehensive immune profiling of the TME of CRC patients revealed that hIgG1-G396R prominently promotes broad mobilization of immune cells (IgG1+ plasma cells, CD8+ T cells, CD103+ DCs) and efficient TLS formation, both key components of an anti-tumor microenvironment. Notably, adoptive transfer of tumor-primed B cells with this variant exhibited therapeutic efficacy in murine tumor models, demonstrating clinical potential. These results prompt a prospective investigation of hIgG1-G396R in CRC patients as a biomarker for clinical prognosis and demonstrate that manipulating the functionality of IgG1+ B cells in tumors could improve immunotherapy outcomes.
Fecal microbiota Profiling in Irritable Bowel Syndrome and Inflammatory Bowel Disease Patients with Irritable Bowel Syndrome-Type Symptoms
Authors: Cui Xiufang , Wang Haiyang , Ye Ziping , Li Yi , Qiu Xinyun , Zhang Hongjie 
Published: January 2021

Abstract
BACKGROUND: The intestinal microbiota is thought to be involved in the occurrence of Inflammatory Bowel Disease in remission (IBDR) with Irritable Bowel Syndrome (IBS)-type symptoms, but the specific distinct profile of these bacteria remains unclear. Therefore, the purpose of this research is to investigate this issue by conducting a cross-sectional study.METHODS: IBS patients were diagnosed according to Rome Ⅳ criteria, IBD diagnosed according to the criteria of European Crohn & Colitis Organization (ECCO), IBDR patients with IBS-type symptoms were defined according to related IBS-type symptoms meeting the Rome IV criteria in IBDR patients, and were included Crohn’s disease in remission (CDR) and ulcerative colitis in remission (UCR) based on Crohn’s Disease Activity Index (DAI) and Mayo Scoring System respectively. Healthy controls come from the physical examination center and exclude people with underlying diseases. All enrolled subjects were divided into six groups, as followed: Health Control, IBS, CDR with IBS-type symptoms (CDR-IBS+), CDR without IBS-type symptoms (CDR-IBS-), UCR-IBS+ and UCR-IBS-. We collected fresh fecal samples from all subjects and applied 16S rRNA sequencing analysis to detect the structure and diversity of the microbiota among different groups. RESULTS: A total of 97 subjects were included in this study, of which 18 were health controls, 34 IBS patients, 25 CDR and 20 UCR. The richness of intestinal microbiota in CDR-IBS-was significantly lower than that in the control and IBS groups based on the analysis of observed species and Chao index (P<0.05). The observed species index in CDR-IBS+ was significantly higher than CDR-IBS- group (median index: 254.8 vs 203, P=0.036). No difference was found in Alpha diversity between UCR-IBS+ and UCR-IBS-. At phylum level, there was no significant difference between UC or CD with IBS-type symptoms and those without related symptoms. At genus level, the number of Faecalibacterium in CDR-IBS+ increased significantly while Fusobacterium decreased compared with CDR-IBS-(mean relative abundance of Faecalibacterium: 20.35% vs 5.18%, P<0.05; Fusobacterium: 1.51% vs 5.2%, P<0.05). However, compared with UCR-IBS - group, the number of Faecalibacterium in UCR-IBS+ group decreased, while the number of Streptococcus increased, but there was no statistical difference in the genus structure. Regardless of the phylum or genus level, the abundance and composition of the microbiota of IBS patients were not distinct from those of healthy people.CONCLUSIONS: CD patients in remission with IBS-type symptoms may be related to the increase of Faecalibacterium and decrease of Fusobacterium. UC patients in remission with IBS-type symptoms cannot be explained by changes in the abundance and structure of intestinal microbiota from our across-sectional study.
Cellulose Intrafibrillar Mineralization of Biological Silica in a Rice Plant
Authors: Nakamura Eri , Ozaki Noriaki , Oaki Yuya , Imai Hiroaki 
Published: January 2021

Abstract
The essence of morphological design has been a fascinating scientific problem with regard to understanding biological mineralization. Particularly shaped amorphous silicas (plant opals) play an important role in the vital activity in rice plants. Although various organic matters are associated with silica accumulation, their detailed functions in the shape-controlled mineralization process have not been sufficiently clarified. In the present study, cellulose nanofibers (CNFs) were found to be essential as a scaffold for silica accumulation in rice husks and leaf blades. Prior to silicification, CNFs ~10 nm wide are sparsely stacked in a space between the epidermal cell wall and the cuticle layer. Silica nanoparticles 20–50 nm in diameter are then deposited in the framework of the CNFs. The shape-controlled plant opals are formed through the interfibrillar mineralization of silica nanoparticles on the CNF scaffold.