bluuebunny/crossref_metadata_2025_split huggingface dataset:
Depletion of H3K36me2 recapitulates epigenomic and phenotypic changes induced by the H3.3K36M oncohistone mutation
Authors: Rajagopalan Kartik N. , Chen Xiao , Weinberg Daniel N. , Chen Haifen , Majewski Jacek , Allis C. David , Lu Chao 
Published: February 2021

SignificanceRecurrent histone H3K36M mutations are found in multiple cancer types yet their oncogenic mechanisms remain incompletely understood. Biochemically, H3K36M oncohistone dominantly inhibits several H3K36-specific methyltransferases such as NSD1/2 and SETD2, resulting in decreases in all methylation states of H3K36. We report here that genetic ablation of NSD1 and NSD2, methyltransferases specific for H3K36 dimethylation (H3K36me2), is sufficient to recapitulate H3K36M’s effects on enhancer activation, gene expression, differentiation blockade, and drug sensitivity. Our results suggest that depletion of H3K36me2 represents a key event downstream of the H3K36M mutation and also exposes potential therapeutic vulnerability of H3K36M-mutant tumor cells.
Hard X-ray omnidirectional differential phase and dark-field imaging
Authors: Wang Hongchang , Sawhney Kawal 
Published: February 2021

Significance
We demonstrate an approach to generate a new type of X-ray imaging mode, which is called omnidirectional X-ray differential phase imaging. The proposed method enables us to detect the subtle phase changes in all directions of the imaging plane, which complements conventional X-ray imaging methods with information that they cannot provide. Importantly, the omnidirectional dark-field images can also be simultaneously retrieved for studying a wide range of complicated samples, particularly strongly ordered systems. The extracted information will not only provide insights into the microarchitecture of materials, but also enrich our understanding the macroscopic behavior. The presented technique could potentially open up numerous practical imaging applications in both biomedical research and materials science.
Management of Kratom Dependence with Buprenorphine/Naloxone in a Veteran Population
Authors: Lei Jamie , Butz Amy , Valentino Natalie 
Published: February 2021

Background Kratom is a substance that when ingested produces an opioid-like effect. As kratom continues to gain popularity, increasing numbers of cases of addiction, dependence, and adverse events have been reported, as well as an increase in mortality associated with its use. George E. Wahlen Department of Veterans Affairs Medical Center has been using buprenorphine/naloxone for the treatment of kratom withdrawal and dependence in both primary care and specialty addiction treatment settings in the Veteran population. Cases We present three cases that describe the use of buprenorphine/naloxone for kratom dependence. For each case, we describe the withdrawal symptoms from kratom, induction and long-term maintenance on buprenorphine/naloxone in kratom dependence, the impact of polysubstance use disorders in management of kratom dependence, and the use of urine drug screens for kratom alkaloids during treatment. Discussion This case series demonstrates that patients with kratom dependence can effectively be treated with buprenorphine/naloxone. It appears that it is safe to induce buprenorphine/naloxone as early as eight hours after last kratom use and maintenance dosing for kratom use was similar to maintenance doses used in opioid use disorder. Prolonged and continued withdrawal symptoms were reported despite treatment with buprenorphine/naloxone and multiple daily doses of up to 24mg per day may be beneficial for prolonged withdrawal symptoms and for cooccurring pain. Polysubstance use with kratom dependence may require higher levels of care and higher doses of buprenorphine/naloxone. Urine drug screens may be best practice for monitoring kratom alkaloids concentrations and facilities that utilize buprenorphine/naloxone to treat kratom dependence should have testing available. Further research is needed on the impact and the treatment of kratom dependence.
New vision for the soliton solutions to the complex Hirota-dynamical model
Authors: Bekir Ahmet , Zahran Emad H M 
Published: February 2021

AbstractIn this paper, the nonlinear complex Hirota-dynamical model NLCHM in which the third derivative term represents the self-interaction in the high-frequency subsystem is established. This model plays a vital role in plasma physics because there are agreements between the self-interaction in the high-frequency and the well- known self-focusing effect in plasma. Many soliton solutions to this equation model have been achieved perfectly using the solitary wave ansatz method (SWAM). Furthermore, in the same vein and related subject the extended simple equation method (ESEM) has been applied perfectly to achieve new perception of soliton solutions to this model. A good isomorphic between the achieved results and that achieved previous by other authors have been listed.
Spectral and temporal characterization of sleep spindles—methodological implications
Authors: Gomez-Pilar Javier , Gutiérrez-Tobal Gonzalo C , Poza Jesús , Fogel Stuart , Doyon Julien , Northoff Georg , Hornero Roberto 
Published: February 2021

Abstract

Objective. Nested into slow oscillations (SOs) and modulated by their up-states, spindles are electrophysiological hallmarks of N2 sleep stage that present a complex hierarchical architecture. However, most studies have only described spindles in basic statistical terms, which were limited to the spindle itself without analyzing the characteristics of the pre-spindle moments in which the SOs are originated. The aim of this study was twofold: (a) to apply spectral and temporal measures to the pre-spindle and spindle periods, as well as analyze the correlation between them, and (b) to evaluate the potential of these spectral and temporal measures in future automatic detection algorithms. Approach. An automatic spindle detection algorithm was applied to the overnight electroencephalographic recordings of 26 subjects. Ten complementary features (five spectral and five temporal parameters) were computed in the pre-spindle and spindle periods after their segmentation. These features were computed independently in each period and in a time-resolved way (sliding window). After the statistical comparison of both periods, a correlation analysis was used to assess their interrelationships. Finally, a receiver operating-characteristic (ROC) analysis along with a bootstrap procedure was conducted to further evaluate the degree of separability between the pre-spindle and spindle periods. Main results. The results show important time-varying changes in spectral and temporal parameters. The features calculated in pre-spindle and spindle periods are strongly and significantly correlated, demonstrating the association between the pre-spindle characteristics and the subsequent spindle. The ROC analysis exposes that the typical feature used in automatic spindle detectors, i.e. the power in the sigma band, is outperformed by other features, such as the spectral entropy in this frequency range. Significance. The novel features applied here demonstrate their utility as predictors of spindles that could be incorporated into novel algorithms of automatic spindle detectors, in which the analysis of the pre-spindle period becomes relevant for improving their performance. From the clinical point of view, these features may serve as novel precision therapeutic targets to enhance spindle production with the aim of improving memory, cognition, and sleep quality in healthy and clinical populations. The results evidence the need for characterizing spindles in terms beyond power and the spindle period itself to more dynamic measures and the pre-spindle period. Physiologically, these findings suggest that spindles are more than simple oscillations, but nonstable oscillatory bursts embedded in the complex pre-spindle dynamics.
Molecularly Imprinted Polymer (MIPs) Nanomaterials Modified as Absorbing Cholesterol
Authors: Sabrina Nadia , Ulianas Alizar , Yulkifli  , Nurlely  
Published: February 2021

Abstract
Nanomaterial Molecularly Imprinted Polymer (MIPs) from Methacrylic acid (MAA), Ethylene glycolmethacrylate (EGDMA), 2,2-Dimetoksi-2-Phenyliacetonephenon (DMPP) and cholesterol have been successfully synthesized by using the photopolymerization method. MIPs has been characterized by Scanning Electron Microscope (SEM) to see the physical shape and size of his fingers, while FTIR was used to analysis of the cholesterol extraction and re-extraction in MIPs. The absorption capacity of MIPs against cholesterol in solution was analyzed by the calorimetry method using Liebermann-Burchard reagent. MIPs absorption of cholesterol in the blood was analyzed by using Easy Touch GCU. The results of SEM analysis showed that MIPs were spherical with a diameter of 1,038µm to 2.702µm. While FTIR analysis shows that MIPs cholesterol can be extracted and re-extracted in MIPs. MIPs absorption against cholesterol in solution is 84.49% for 0.30 grams of MIPs and time of absorbance is 30 minutes. While the absorption of MIPs against cholesterol in the human blood is 41.19%, it shows that pores and active groups of MIPs are able to absorb and selectively to cholesterol molecules.
Molecular encapsulation of bioactive molecules of Ruku-Ruku leaves (Ocimum tenuiflorum Linnen) as a preliminary stability study
Authors: Parbuntari H , Etika S B , Delvia E 
Published: February 2021

Abstract
Ruku-ruku (RK) leaves in West Sumatra contain various essential oil which is known as secondary metabolites. They could influence the use of these bioactive properties as medicinal compounds. Essential oils have unstable properties to oxidation, evaporation, or chemical reaction. One method for maintaining compound stability is the encapsulation method. This study performs the encapsulation of RK in β-CD. By using the co-precipitation method, this study produces pure crystal with 62,5% of the efficiency of encapsulation. The diffraction pattern of β-CD: RK identified the crystals pattern with a sharp peak of diffraction and most of these peaks showed patterns of diffraction in β-CD. The absorption intensity of the pure CD was only 2.5 but after inserting the RK in its cavity the intensity increased to 2.7. It shows that there is an interaction between the non-covalent part of β-CD and the essential oil molecules. Furthermore, the inclusion complex β-CD: RK is more stable as the increase in the boiling point of the ICs in the range of 282-289°C.
The Effect of Multiple Electrode Pairs to Electricity Potential of Ceramic-Based and Tempe Waste Microbial Fuel Cell
Authors: Sejati Emut Sukma , Sudarlin  
Published: February 2021

Abstract
Ceramic is low-cost separator membrane has widely applied in dual-chambered Microbial Fuel Cell (MFC). Its big pores and other chemical and physical advantages make it suitable to substitute expensive exchange separator membranes. The purpose of this study is to enhance electricity of ceramic-based microbial fuel cell by using various number of carbon electrode pairs. This study uses tempe waste as anolyte and KMnO4 as its catholyte to gain electricity. Three variety of electrode pairs data of electricity result is processed statistically to examine significant difference of voltage, current, and power density as parameters. The result of this study shows that electricity of three-electrode pairs has a higher average of power density with the number 1447,91 mW/m2, the difference between three and two electrode pairs is around 588 mW/m2 and between three and one electrode pairs is 910 mW/m2. It has significant difference between one, two, and three-electrode pairs in the parameters.
Optimization of hexavalent chromium ion adsorption using natural silica modified with DMA (Dimethylamine) by batch method
Authors: Weni Nora Kemala , Oktavia Budhi 
Published: February 2021

Abstract
Heavy metals are the most dangerous source of pollution for environmental systems because they have non-biodegradable, toxic, and even carcinogenic properties even in low concentrations (ppm). Usually metals are insoluble, precipitated, dissolved, absorbed, inorganic, reduced, oxidized, and free of metals. One of the heavy metals that can cause negative effects on humans and living things is chromium ion. One way to overcome chromium metal in waste is to absorb it with DMA modified silica adsorbent. The adsorption of chromium (VI) from aqueous solutions was examined at various pH, contact times, and initial Cr (VI) concentrations. Langmuir adsorption isotherm yields a regression coefficient R2 = 0.9807. In the initial conditions Cr (VI) 100 mg L-1, pH 2.0 with stirring for 90 minutes, the maximum adsorption capacity of Cr (VI) was 1,024 mg g-1. In this study, researchers used natural silica modified with dimethylamine as an adsorbent for the adsorption of Cr (VI) ion, GPTMS (glycidoxypropyltrimethoxilane) as an intermediate or bridge connecting silica with DMA (modifying compound).
Hydrophobic Pocket of SARS-Cov-2 Spike Glycoprotein are Potential as Binding Pocket
Authors: Fitri A , Basultan H , Iryani  
Published: February 2021

Abstract
Coronavirus disease 2019 (COVID-19) caused by SARS-Cov-2 was recently spread all over the world. Spike glyprotein of SARS-Cov-2 (SARS-Cov-2 S-glycoprotein) is the main agent for host cell recognition. Finding the potential of binding pocket of S-glycoprotein may help to find the specific anti-coronavirus drug. Here we analysed potential binding pocket of SARS-Cov-2 Spike-glycoprotein which is suitable for anti-SARS-Cov-2. In pursuit this aim, dogsitescorer, site finder, and DEPTH were used for binding pocket prediction. Molecular interaction protein-ligands were performed using MOE 2009.10. Based on pocket prediction by Dogsitescorer, there are seven out of eleven pockets which have druggability score above 0.8. Molecular interaction studies revealed that interaction between six potential pockets and ligands resulted in negative scores at all. Our result shows that pocket_4 and pocket_6 are located on upper of SARS-Cov-2 S-glycoprotein and have big volume, 878.94 and 683.05 (Å3) respectively, yet lower number of hydrogen bond. Hydrophobic pocket zero, three, and five which is located in the middle of S-Glycoprotein have high number of interaction. These suggest that hydrophobicity of pocket and both upper and middle positions of S-Glycoprotein pocket are considered for developing anti-coronavirus drugs. We propose that hydrophobic pocket of SARS-Cov-2 S-glycoprotein is important for drug design.
Effect of Using Guided Inquiry-Based Chemical Bonding Modules on Student Learning Outcomes
Authors: Iryani  , Bayharti  , Iswendi  , Putra Regi Fadila 
Published: February 2021

Abstract
This Study is a continuation of Study on the development of guided inquiry-based chemical bond modules that have been tested for validity and practicality, but have not yet been tested for its effects. This study purposed to reveal the influence of using guided inquiry-based chemical bonding modules on the learning outcomes of class X students of SMAN 4 Padang and SMAN 9 Padang. The data of the two schools of them are normally distributed and homogeneous. T-test results at the significant level of 0.05, namely t-count (1.79) > t-table (1.67) on SMAN 4 Padang and (2.93) > t-table (1.66) on SMAN 9 Padang. Based on the analysis it appears that student learning outcomes using the guided inquiry-based chemical bond module is increase significantly.
Cross-sectional approach for diagnosing student’s learning progression in relation to pre-requisite knowledge of buffer solution
Authors: Fitriza Z , Aini F Q , Handayani P 
Published: February 2021

Abstract
Buffer Solution is one of the complex concepts in chemistry since it involves many prerequisite concepts such as acid and base, chemical equilibrium, and stoichiometry. These concepts also need sequent students’ understanding of the chemical equation, chemical nomenclature, and atomic symbol. Their difficulty in comprehending prerequisite knowledge will affect the learning progression. This research aims to diagnose learning progression and the causes of the learning progression block of senior high school students on the buffer solution. Learning progression of 82 X grade, 80 XI grade, and 80 XII grade students were identified by a structured diagnostic test of chemistry using a cross-sectional approach. This research figure out that the learning progression of the student was not as expected since they cannot mastering buffer solution well particularly in buffer solution component, pH of buffer solution, and pH maintaining mechanism because of their difficulty in understanding chemical equilibrium and ionization reaction.
The Development and Validity of CPS-Based Assessment Instrument for Critical Thinking Ability on Stoichiometry Materials
Authors: Agustini  , Rery R U , Anwar L 
Published: February 2021

AbstractEducation at schools, especially high schools, has not been handled so well that critical thinking skills of the high school graduates are still relatively low. An assessment is necessary to find out whether the on-going teaching and learning process has achieved the stated learning objectives. Thus, it is necessary to develop an instrument that can evaluate the students’ critical thinking abilities. This study aimed to determine the validity of CPS-based assessment instrument for students’ critical thinking skills on stoichiometry materials. Problem Solving-based learning is one of the learning methods recommended by the government, where the learning process emphasizes the independence of students in solving problems. The assessment instrument was developed to measure students’ critical thinking skills based on 12 indicators of critical thinking according to Ennis and the characteristics of CPS according to Vidal, namely finding problems in general, finding facts, clarifying problems, expressing opinions, finding solutions and implementing them. The method of development adapted from Borg & Gall Model which consists of 10 development steps. However, this study was conducted only in three stages, namely preliminary research and information gathering, planning, and development of the initial product. The results of this study indicated that the CPS-based assessment instrument fulfilled the validity requirements, not only material validity but also language and construct validity. This study was expected to produce a valid instrument so that it could be used to assess the students’ critical thinking ability.
Development of A Test Instrument to Measure Hots and Cognitive Knowledge in Biology
Authors: Septiani V , Paidi  
Published: February 2021

Abstract
SKL of primary and secondary education set each graduate have HOTS (analysis, evaluation, and create) and cognitive knowledge (factual, conceptual, procedural, and metacognitive). The aim of this research is to develop test instrument of multiple choice for measuring how the ability to HOTS and cognitive knowledge of biology of senior high school students on the subject of human digestive system. The type of this research is Research and Development (R&D) that based on the development model of Oriondo & Dallo-Antonio and Wilson. The field testing was be done in March-April 2020 involving 280 around the five state senior high school in Yogyakarta. The data based on analysis measurement Rasch Model and use Quest program to find out goodness of fit, difficulty index, and reliability. Result of analysis shows that test instrument of HOTS and Cognitive Knowledge were valid and reliable. The goodness of fit test for the overall test and each item shows fit with the Rasch model. Difficulty indexes for the item ranged -3,44 to +1,36. Reliabilities of item estimate were 0,99.
Design of e-modules to stimulate HOTS on static fluid materials with the STEM approach
Authors: Afriyanti M , Suyatna A , Viyanti  
Published: February 2021

Abstract
This study aims to develop an e-module to stimulate HOTS on static fluid material with the STEM approach. The research method uses the design of research and development (R&D) using the ADDIE model which consists of five stages, namely: analysis, design, development, implementation, and evaluation. This article reported two stages namely analysis and design. The instrument used in the form of a questionnaire of product needs analysis and practitioners’ validation sheets using a Likert scale. Source of data for needs analysis are senior high 40 school students and 18 physics educators in Lampung. Product design validation is carried out by qualified and professional physics educators in their fields with the Masters in Physics Education category. Data were collected using Google Form and analyzed using descriptive quantitative. The results showed that an e-module to stimulate HOTS on static fluid material was very needed in the field. In conclusion, the e-module design is very suitable for stimulating HOTS and can be done at the stage of developing an interactive e-module based on STEM to stimulate HOTS of students on static fluid material.
Validity testing of a contextual approached with REACT components-based module on topic of salt hydrolysis for class XI senior high school students
Authors: Gazali Fauzana , Yusmaita Eka , Feronika Nur Intan 
Published: February 2021

Abstract
A contextual approach-based module that consists of five components namely Relating, Experiencing, Applying, Cooperating, and Transferring (REACT) can help students find concepts. This research aims to develop a contextual approach-based module that contains critical thinking skills indicators on salt hydrolysis topics which have content validity and construct validity. Research instruments were construct validity questionnaires analyzed with Aiken V formula and a content validity questionnaire analyzed with CVR approach. The questionnaires were filled by 3 chemistry lecturers and 2 chemistry teachers at SMA 9 Padang. It was found that the average of V score for construct validity was 0.81 showing valid categories, while the CVR and CVI values of content validity were 0.98 and 1 respectively also showing valid categories. Therefore, it can be concluded that the salt hydrolysis module which is based on a contextual approach with REACT components for class XI learning is valid.
The development of guided inquiry based student worksheet of chemical equilibrium towards student activities
Authors: Kardena Hidayati , Mawardi Mawardi 
Published: February 2021

Abstract
This study aims to produce a worksheet of chemical equilibrium which based on guided inquiry to determine the validity, practicality and effectiveness the student worksheet on student learning activities. Learning activities are assessed in the form of listening activities, visual activities, mental activities, oral activities, writing activities and motor activities. This research method was a research development using the Plomp model. The research subjects were students majoring in chemistry in 2019. The research instruments used were validation sheets, practicality sheets and observation sheets of learning activities. This research found that the students’ worksheets have a very high level of validity (k=0,91) and very high level of practicality by students (k=0,87) and high by the lecturer (k=0,79). The results showed that in general, the average student learning activity was 81,18% which was included in the very high category. It shows that the students’ worksheets which have been produced can be used as one of the teaching materials in the learning process on chemical equilibrium material.