bluuebunny/crossref_metadata_2025_split huggingface dataset:
Development, scrutiny, and modulation of transient reporter gene assays of the xenobiotic metabolism pathway in zebrafish hepatocytes
Authors: Lungu-Mitea Sebastian , Han Yuxin , Lundqvist Johan 
Published: October 2021

AbstractThe “toxicology in the twenty-first century” paradigm shift demands the development of alternative in vitro test systems. Especially in the field of ecotoxicology, coverage of aquatic species-specific assays is relatively scarce. Transient reporter gene assays could be a quick, economical, and reliable bridging technology. However, the user should be aware of potential pitfalls that are influenced by reporter vector geometry. Here, we report the development of an AhR-responsive transient reporter-gene assay in the permanent zebrafish hepatocytes cell line (ZFL). Additionally, we disclose how viral, constitutive promoters within reporter-gene assay cassettes induce squelching of the primary signal. To counter this, we designed a novel normalization vector, bearing an endogenous zebrafish-derived genomic promoter (zfEF1aPro), which rescues the squelching-delimited system, thus, giving new insights into the modulation of transient reporter systems under xenobiotic stress. Finally, we uncovered how the ubiquitously used ligand BNF promiscuously activates multiple toxicity pathways of the xenobiotic metabolism and cellular stress response in an orchestral manner, presumably leading to a concentration-related inhibition of the AhR/ARNT/XRE-toxicity pathway and non-monotonous concentration–response curves. We named such a multi-level inhibitory mechanism that might mask effects as “maisonette squelching.”
Graphical abstract
A transient reporter gene assay in zebrafish cell lines utilizing endogenous regulatory gene elements shows increased in vitro toxicity testing performance.
Synthetic and constitutive promotors interfere with signal transduction (“squelching”) and might increase cellular stress (cytotoxicity).
The squelching phenomenon might occur on multiple levels (toxicity pathway crosstalk and normalization vector), leading to a complete silencing of the reporter signal.
Children’s Experiences of Stress in Joint Physical Custody
Authors: Augustijn Lara 
Published: October 2021

Abstract
Background
Joint physical custody is a parental care arrangement in which children live roughly an equal amount of time with each parent after family dissolution, residing alternately in each of the two parental households. Because joint physical custody is characterised by fathers’ continued involvement in their children’s lives, this care arrangement is believed to compensate for the negative effects of family dissolution, and to contribute to children’s well-being in post-separation families.

Objective
This study aims to investigate potential differences in the experiences of stress of children living in joint physical custody and sole physical custody arrangements, while considering both the proportion of time the children spend with each of their parents and the number of transitions the children make between the parental households.

Methods
Based on data from the Family Models in Germany (FAMOD) study, a national convenience sample, linear regression models were estimated for 297 children between the ages of 11 and 14.

Results
The statistical analysis suggests that there was no significant association between the physical custody type (sole physical custody vs. joint physical custody) and the children’s levels of stress. In addition, the results revealed that children’s experiences of stress did not depend on how often they moved between their parents’ households.

Conclusions
This study does not corroborate the assumption that joint physical custody has a protective effect on children, but instead suggests that different physical custody arrangements are associated with different stressors that lead to similar levels of stress in children living in different post-separation families.
Natural variability vs forced signal in the 2015–2019 Central American drought
Authors: Pascale Salvatore , Kapnick Sarah B. , Delworth Thomas L. , Hidalgo Hugo G. , Cooke William F. 
Published: October 2021

AbstractThe recent multi-year 2015–2019 drought after a multi-decadal drying trend over Central America raises the question of whether anthropogenic climate change (ACC) played a role in exacerbating these events. While the occurrence of the 2015–2019 drought in Central America has been asserted to be associated with ACC, we lack an assessment of natural vs anthropogenic contributions. Here, we use five different large ensembles—including high-resolution ensembles (i.e., 0.5∘ horizontally)—to estimate the contribution of ACC to the probability of occurrence of the 2015–2019 event and the recent multi-decadal trend. The comparison of ensembles forced with natural and natural plus anthropogenic forcing suggests that the recent 40-year trend is likely associated with internal climate variability. However, the 2015–2019 rainfall deficit has been made more likely by ACC. The synthesis of the results from model ensembles supports the notion of a significant increase, by a factor of four, over the last century for the 2015–2019 meteorological drought to occur because of ACC. All the model results further suggest that, under intermediate and high emission scenarios, the likelihood of similar drought events will continue to increase substantially over the next decades.
Multi-messenger astrophysics with THESEUS in the 2030s
Authors: Ciolfi Riccardo , Stratta Giulia , Branchesi Marica , Gendre Bruce , Grimm Stefan , Harms Jan , Lamb Gavin Paul , Martin-Carrillo Antonio , McCann Ayden , Oganesyan Gor , Palazzi Eliana , Ronchini Samuele , Rossi Andrea , Salafia Om Sharan , Salmon Lana , Ascenzi Stefano , Capone Antonio , Celli Silvia , Dall’Osso Simone , Di Palma Irene , Fasano Michela , Fermani Paolo , Guetta Dafne , Hanlon Lorraine , Howell Eric , Paltani Stephane , Rezzolla Luciano , Vinciguerra Serena , Zegarelli Angela , Amati Lorenzo , Blain Andrew , Bozzo Enrico , Chaty Sylvain , D’Avanzo Paolo , De Pasquale fnmMassimiliano , Dereli-Bégué Hüsne , Ghirlanda Giancarlo , Gomboc Andreja , Götz Diego , Horvath Istvan , Hudec Rene , Izzo Luca , Le Floch Emeric , Li Liang , Longo Francesco , Komossa S. , Kong Albert K. H. , Mereghetti Sandro , Mignani Roberto , Nathanail Antonios , O’Brien Paul T. , Osborne Julian P. , Pe’er Asaf , Piranomonte Silvia , Rosati Piero , Savaglio Sandra , Schüssler Fabian , Sergijenko Olga , Shao Lijing , Tanvir Nial , Turriziani Sara , Urata Yuji , van Putten Maurice , Vergani Susanna , Zane Silvia , Zhang Bing 
Published: October 2021

AbstractMulti-messenger astrophysics is becoming a major avenue to explore the Universe, with the potential to span a vast range of redshifts. The growing synergies between different probes is opening new frontiers, which promise profound insights into several aspects of fundamental physics and cosmology. In this context, THESEUS will play a central role during the 2030s in detecting and localizing the electromagnetic counterparts of gravitational wave and neutrino sources that the unprecedented sensitivity of next generation detectors will discover at much higher rates than the present. Here, we review the most important target signals from multi-messenger sources that THESEUS will be able to detect and characterize, discussing detection rate expectations and scientific impact.
Subdomain-based exponential integrators for quantum Liouville-type equations
Authors: Schulz L. , Inci B. , Pech M. , Schulz D. 
Published: October 2021

AbstractIn order to describe quantum mechanical effects, the use of the von-Neumann equation is apparent. In this work, we present a unified numerical framework so that the von-Neumann equation in center-of-mass coordinates leads to a Quantum Liouville-type equation when choosing a suitable basis. In particular, the proposed approach can be related to the conventional Wigner equation when a plane wave basis is used. The drawback of the numerical methods is the high computational cost. Our presented approach is extended to allow reducing the dimension of the basis, which leads to a computationally efficient and accurate subdomain approach. Not only the steady-state behavior is of interest, but also the dynamic behavior. In order to solve the time-dependent case, suitable approximation methods for the time-dependent exponential integrator are necessary. For this purpose, we also investigate approximations of the exponential integrator based on Faber polynomials and Krylov methods. In order to evaluate and justify our approach, various test cases, including a resonant tunnel diode as well as a double-gate field-effect transistor, are investigated and validated for the stationary and the dynamic device behavior.
What Does Good Science-Based Advice to Politics Look Like?
Authors: Carrier Martin 
Published: October 2021

AbstractI address options for providing scientific policy advice and explore the relation between scientific knowledge and political, economic and moral values. I argue that such nonepistemic values are essential for establishing the significance of questions and the relevance of evidence, while, on the other hand, such social choices are the prerogative of society. This tension can be resolved by recognizing social values and identifying them as separate premises or as commissions while withholding commitment to them, and by elaborating a plurality of policy packages that envisage the implementation of different social goals. There are limits to upholding the value-free ideal in scientific research. But by following the mentioned strategy, science can give useful policy advice by leaving the value-free ideal largely intact. Such scientific restraint avoids the risk of appearing to illegitimately impose values on the public and could make the advice given more trustworthy.
Trends in Health Equity Among Children in the United States, 1997–2018
Authors: Anderson Nathaniel W. , Zimmerman Frederick J. 
Published: October 2021

Abstract
Objectives
Health equity is crucial to population health. To achieve this aim, extensive monitoring efforts beyond traditional disparities research are required. This analysis assesses trends in health equity for children from 1997 to 2018.

Methods
Health equity in a given year is calculated using a previously developed measure as the mean weighted departure of individual health from the best achievable level of health. This criterion is defined as the median health of the most socially privileged identifiable group: white, non-Latinx boys in upper-income households.
Using more than 20 years of data from the National Health Interview Survey, we apply this methodology to six measures of child health: parent-reported health status, school days missed due to illness or injury in the past year, a strength and difficulties questionnaire score, emotional difficulties, a toddler mental health indicator score, and toddler depression. We separately calculate racial/ethnic and income disparities.
Monte Carlo simulation is used to assess whether trends are statistically significant.

Results
Health equity among children increased gradually over the past 2 decades, with five of the six measures demonstrating upward trends. Improvements in health equity are stronger among younger children (age 0–3 and 4–7). Unlike previous work examining adults, both types of disparities narrowed over the study period.

Conclusions for Practice
Progress on health equity requires accountability to an objective metric. This analysis suggests some improvement over the past two decades, although these gains are under threat from potential decreases in government spending on programs affecting children and the COVID-19 pandemic.
Sharpness of the Phase Transition for the Orthant Model
Authors: Beekenkamp Thomas 
Published: October 2021

AbstractThe orthant model is a directed percolation model on $\mathbb {Z}^{d}$

ℤ

d

, in which all clusters are infinite. We prove a sharp threshold result for this model: if p is larger than the critical value above which the cluster of 0 is contained in a cone, then the shift from 0 that is required to contain the cluster of 0 in that cone is exponentially small. As a consequence, above this critical threshold, a shape theorem holds for the cluster of 0, as well as ballisticity of the random walk on this cluster.
The Effects of Politician’s Moral Violations on Voters' Moral Emotions
Authors: Walter Annemarie S. , Redlawsk David P. 
Published: October 2021

AbstractExisting empirical research on voters’ responses to individual politicians’ moral transgressions pays limited attention to moral emotions, although moral emotions are an integral part of voters’ moral judgment. This study looks at U.S. voters’ discrete moral emotional responses to politician’s moral violations and examines how these discrete moral emotional responses are dependent on voters’ own moral principles and the extent to which they identify with a political party. We report on a 5 × 3 between-subjects experiment where 2026 U.S. respondents reacted to politicians’ violations of one of five moral foundations defined by Moral Foundations Theory. We randomly vary which moral foundation is violated and the partisanship of the politician. While voters’ own moral principles somewhat condition moral emotional responses, we find that voters’ moral emotional responses mostly depend on partisan identification. When voters share party identity with a politician committing a moral violation, they respond with less anger, contempt, disgust and shame than when they do not share party identity. The effect is greater among strong partisans. However, we find limited evidence that specific moral emotions are activated by violations of particular moral foundations, thereby challenging Moral Foundations Theory.
End-of-life tire management: a critical review
Authors: Dabic-Miletic Svetlana , Simic Vladimir , Karagoz Selman 
Published: October 2021

AbstractEnvironmental and social awareness are the key elements of the sustainable tire industry. End-of-life tire (ELT) waste flow is an important environmental problem worldwide since it produces severe air, water, and soil pollution issues. Significant advancements have been made in ELT management in the last few years. As a result, ELTs should not only be regarded as waste but also as a source of environmentally friendly materials. Besides, sound ELT management has vital importance for circular economy and sustainable development. Over the last decade, ELT management has attracted many researchers and practitioners. Unfortunately, a comprehensive review of the ELT management area is still missing. This study presents the first critical review of the whole ELT management area. It aims to present an extensive content analysis overview of state-of-the-art research, provide its critical analysis, highlight major gaps, and propose the most significant research directions. A total of 151 peer-reviewed studies published in the journals between 2010–2020 are collected, analyzed, categorized, and critically reviewed. This review study redounds comprehensive insights, a valuable source of references, and major opportunities for researchers and practitioners interested in not only ELT material flow but also the whole waste management area.
Graphical abstract
Effect of Mn on Microstructure and Mechanical Properties of Al-4Ni Alloy
Authors: Fang Jiao , Dong Xixi , Ji Shouxun 
Published: October 2021

AbstractThe application of aluminum alloys at elevated temperatures has been attractive for decades, and Al-Ni-based alloys have recently been recognized as potential candidates. The effect of Mn on Al-4Ni alloy has been investigated in this work. Addition of Mn transformed the eutectics from Al3Ni/α-Al to Al9(Ni,Mn)2/α-Al phases. Mn also improved the tensile strength at both 25°C and 250°C. The yield strength at 25°C increased from 48 MPa to 92 MPa with 1.87% Mn and then to 117 MPa with 3.77% Mn. At 250°C, the yield strength increased from 35 MPa to 82 MPa with 1.87% Mn and then to 101 MPa with 3.77% Mn. The alloys with Mn also showed less strength loss than Al-4Ni alloy at 250°C. The eutectic Al9(Ni,Mn)2 phase showed good thermal stability. No coarsening was observed after 2000 h at 250°C.
Generation of an Lpar1-EGFP Fusion Knock-in Transgenic Mouse Line
Authors: Rivera Richard , Williams Nyssa A. , Kennedy Grace G. , Sánchez-Pavón Paloma , Chun Jerold 
Published: October 2021

AbstractLysophosphatidic acid (LPA) is a lysophospholipid that acts as an extracellular signal through the activation of cognate G protein-coupled receptors (GPCRs). There are six known LPA receptors (LPA1–6). The first such receptor, LPA1, was identified in the embryonic brain and has been studied extensively for gene expression throughout the body, including through studies of receptor-null mice. However, identifying receptor protein expression in situ and in vivo within living cells and tissues has been difficult because of biologically low receptor expression and variable antibody specificity. To visualize native LPA1 receptor expression in situ, we generated a knock-in mouse produced by homologous recombination in murine embryonic stem (ES) cells to replace a wildtype Lpar1 allele with a mutant allele created by in-frame fusion of EGFP to the 4th exon of Lpar1 (Lpar1-EGFP knock-in allele). Homozygous knock-in mice appeared normal and the expected mendelian ratios of knock-in allele transmission were present in females and males. Histological assessments of the fetal and adult central nervous system (CNS) demonstrated expression patterns that were consistent with prior in situ hybridization studies. This new mouse line will be useful for studies of LPA1 in the developing and adult CNS, as well as other tissues, and for receptor assessments in living tissues and disease models.
Breccia interlayer effects on steam-assisted gravity drainage performance: experimental and numerical study
Authors: Zhang Qichen , Kang Xiaodong , Liu Huiqing , Dong Xiaohu , Wang Jian 
Published: October 2021

AbstractCurrently, the reservoir heterogeneity is a serious challenge for developing oil sands with SAGD method. Nexen’s Long Lake SAGD project reported that breccia interlayer was widely distributed in lower and middle part of reservoir, impeding the steam chamber expansion and heated oil drainage. In this paper, two physical experiments were conducted to study the impact of breccia interlayer on development of steam chamber and production performance. Then, a laboratory scale numerical simulation model was established and a history match was conducted based on the 3D experimental results. Finally, the sensitivity analysis of thickness and permeability of breccia layer was performed. The influence mechanism of breccia layer on SAGD performance was analyzed by comparing the temperature profile of steam chamber and production dynamics. The experimental results indicate that the existence of breccia interlayer causes a thinner steam chamber profile and longer time to reach the peak oil rate. And, the ultimate oil recovery reduced 15.8% due to much oil stuck in breccia interlayer areas. The numerical simulation results show that a lower permeability in breccia layer area has a serious adverse impact on oil recovery if the thickness of breccia layer is larger, whereas the effect of permeability on SAGD performance is limited when the breccia layer is thinner. Besides, a thicker breccia layer can increase the time required to reach the peak oil rate, but has a little impact on the ultimate oil recovery.
Plasmon Assisted Highly Efficient Visible Light Catalytic CO2 Reduction Over the Noble Metal Decorated Sr-Incorporated g-C3N4
Authors: Humayun Muhammad , Ullah Habib , Shu Lang , Ao Xiang , Tahir Asif Ali , Wang Chungdong , Luo Wei 
Published: October 2021

AbstractThe photocatalytic performance of g-C3N4 for CO2 conversion is still inadequate by several shortfalls including the instability, insufficient solar light absorption and rapid charge carrier’s recombination rate. To solve these problems, herein, noble metals (Pt and Au) decorated Sr-incorporated g-C3N4 photocatalysts are fabricated via the simple calcination and photo-deposition methods. The Sr-incorporation remarkably reduced the g-C3N4 band gap from 2.7 to 2.54 eV, as evidenced by the UV–visible absorption spectra and the density functional theory results. The CO2 conversion performance of the catalysts was evaluated under visible light irradiation. The Pt/0.15Sr-CN sample produced 48.55 and 74.54 µmol h−1 g−1 of CH4 and CO, respectively. These amounts are far greater than that produced by the Au/0.15Sr-CN, 0.15Sr-CN, and CN samples. A high quantum efficiency of 2.92% is predicted for the Pt/0.15Sr-CN sample. Further, the stability of the photocatalyst is confirmed via the photocatalytic recyclable test. The improved CO2 conversion performance of the catalyst is accredited to the promoted light absorption and remarkably enhanced charge separation via the Sr-incorporated mid gap states and the localized surface plasmon resonance effect induced by noble metal nanoparticles. This work will provide a new approach for promoting the catalytic efficiency of g-C3N4 for efficient solar fuel production.
Integration Analysis of Electric Vehicle Charging Station Equipped with Solar Power Plant to Distribution Network and Protection System Design
Authors: Turan Mehmet Tan , Gökalp Erdin 
Published: October 2021

AbstractThe improvements and implementation of new generation equipment to the conventional grids are increasing along with the increase of the number of renewable energy power plants and smart grid components. The existing grid infrastructure is required to be modified and upgraded as a result of the increasing integration of the renewables and new generation consumers where the availability of the existing networks has to be analyzed for integration as well. In order to keep the grid stable and integrate the renewables to the grid optimally, necessary analyses have to be realized including the investigation of the optimal integration criteria. During the integration phase of the new generation consumers and producers, two ways power flows and variable power production of renewables have to be considered for system design. In this study, analysis for optimal sizing and integration studies are performed for electric vehicle parking lot and solar power plants located on the campus distribution network considering optimal sizing criteria and the aim of stabilization of voltage regulation during day time operation of solar power plant and random charging profile of electric vehicles. The proposed network is equipped with adaptive protection system to ensure stability and the reliability of the distribution network in the concept of smart grid.