bluuebunny/crossref_metadata_2025_split huggingface dataset:
Association Between CACNA1C Polymorphisms and Insomnia in a Chinese Han Population: A Case-Control Population-Based Analysis
Authors: Chen Liucui , Li Huaxin , Yuan Haijun , Gao Wei , Liu Feng , Ma Bing , Ding Yingmei , Liu Xiaofen , Yuan Mei 
Published: June 2021

Abstract
A limited number of genome-wide association studies (GWAS) have shown that CACNA1C is a potential candidate gene for insomnia. The present study sought to investigate the association of CACNA1C gene polymorphisms and insomnia in a Chinese Han population. Twenty-one single nucleotide polymorphisms (SNPs) in the CACNA1C were genotyped by using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) with a Sequenom MassARRAY system in 205 patients with insomnia and 154 healthy controls. Data from all participants were systematically collected. Association of the polymorphisms with insomnia was evaluated by statistical analysis. Linkage disequilibrium (LD) and haplotype analysis were performed with Haploview v4.2 software. After adjustment for multiple comparisons and gender, age, body mass index (BMI), hypertension and glycated hemoglobin (HbA1c), only the rs2302729 in the recessive model (adjusted odds ratio [AOR]= 0.414, 95%CI= 0.220-0.771, P= 0.004) related to insomnia achieved significance by the false discovery rate (FDR) Benjamini and Hochberg (BH) criterion. The haplotypes rs2302729-rs1051375, CA and TG were significantly associated with insomnia (P<0.05). Our findings contributed important evidence for the confirming of CACNA1C as a susceptibility gene for insomnia in the samples of Chinese Han population.
Human Development, Greenhouse Gas Emissions and Sub-National Mitigation Burdens: A Brazilian Perspective
Authors: Ambrósio Geanderson , Cunha Dênis Antônio Da , Pires Marcel Viana , Costa Luis , Faria Raiza Moniz , Gurgel Angelo Costa 
Published: June 2021

Abstract
International frameworks for greenhouse gas (GHG) mitigation usually disregard country-specific inequalities for the allocation of mitigation burdens. This may hinder low developed regions in a country from achieving development in a socioeconomic perspective, such as the Sustainable Development Goals (SDGs) of eradicating poverty (SDG1) and hunger (SDG2). We use observed data (1991-2010) of carbon dioxide equivalent (CO2eq) emissions and a sub-national human development index (MicroHDI, range [0, 1]) for Brazilian microregions to design a framework where regional mitigation burdens are proportional to the MicroHDI, without compromising national mitigation pledges. According to our results, the less developed Brazilian regions have not been basing their development in emission-intensive activities; instead, the most developed regions have. Between 2011 and 2050, Brazilian cumulative emissions from the sectors most correlated with MicroHDI are expected to be 325 Gt CO2eq, of which only 50 Gt are associated with regions of MicroHDI < 0.8. Assuming a national GHG mitigation target of 56.5% in 2050 over 2010 (consistent with limiting global warming to 2ºC), Brazil would emit 190Gt CO2eq instead of 325Gt and the 135 Gt reduction is only accounted for by regions after reaching MicroHDI ≥ 0.8. Allocating environmental restrictions to the high-developed regions leaves ground for the least developed ones to pursue development with fewer restrictions. Our heterogeneous framework represents a fairer allocation of mitigation burdens and could be an international reference for addressing both environmental and socioeconomic development in developing countries at sub-national level as emphasized by the SDGs.
Structure-based screening of Natural product libraries in search of potential antiviral drug-leads as first-line treatment to Covid-19 infection
Authors: Rao Aditya , Shetty Nandini 
Published: June 2021

Abstract
The study describes a novel strategy to screen natural products (NPs) based on their structural similarities with chemical drugs and their use as first-line treatment to Covid-19 infection. In the present study, the in-house natural product libraries, consisting of a total of 26,311 structures, were screened against potential targets of 2019-nCoV/SARS-CoV-2 based on their structural similarities with the prescribed chemical drugs. The comparison was based on molecular properties, 2 and 3-dimensional structural similarities, activity cliffs, and core fragments of NPs with chemical drugs. The screened NPs were evaluated for their therapeutic effects based on predicted in-silico pharmacokinetic and pharmacodynamics properties, binding interactions with the appropriate targets, and structural stability of the bound complex. The study yielded NPs with significant structural similarities to synthetic drugs currently used to treat Covid-19 infections. The study proposes the selected NPs as Anti-retroviral protease inhibitors, RNA-dependent RNA polymerase inhibitors, and viral entry inhibitors.
Effects of provenance, pre-treatment and seeds’ mass on germinability and seedling growth of socio-economically important Balanites aegyptiaca (L.) Delile and Ricinodendron heudelotii (Bail.) Pierre in Benin (West Africa)
Authors: HOUNSOU-DINDIN Guillaume , Idohou Rodrigue , Hounsode Marcel T. Donou , Adomou Aristide C. , Assogbadjo Achille E. , Kakaï Romain Glèlè 
Published: June 2021

Abstract
Balanites aegyptiaca (L.) Delile and Ricinodendron heudelotii (Bail.) Pierre are socioeconomically important but endemic species to sub-Saharan Africa. This study was conducted to assess the germination capacity of their seeds and seedling growth according to seed provenance, seed mass and pre-treatment techniques as a contribution to the development of strategies for their conservation and domestication in Benin. The seeds were randomly collected in the species occurrence phytodistricts. A split-split plot design with three replicates was used. The survival analysis and generalized linear mixed effects models were implemented on the data. Findings were that the heaviest seeds ( B. aegyptiaca seed mass ≥ 3 g and R. heudelotii ≥ 1.50 g) , provided the highest germination rates (73.60 ± 5.19% and 62.50 ± 5.71%) with seeds scarified with a hammer first emerging at day-8 and day-10 for B. aegyptiaca and R. heudelotii respectively. For B. aegyptiaca seedlings, the seeds from North Borgou phytodistrict scarified with a hammer and the heaviest seeds showed the highest total height (36.43 ± 1.03 cm), basal diameter (2.84 ± 0.03 mm), the greatest number of leaves (32) and ramifications (1). The heaviest seeds of R. heudelotii showed also the highest total height from the day-28 after sowing (26.73 ± 13.56 cm) until the day-105 (151.97 ± 6.37 cm) and those from Pobe phytodistrict showed the highest basal diameter (12.53 ± 1.47 mm) and the greatest number of leaves (14), with almost no ramification during the trial period. These findings constitute a step towards upscaling the reproducibility of these species for better contribution to economies while serving for restoration plans.
Identification and characterization of a novel NAC transcription factor gene from triticale (x Triticosecale Wittmack)
Authors: Wang Meng , Ren Litong , Wei Xiaoyong , Ling Yueming , Gu Haitao , Wang Shanshan , KONG Guagnchao 
Published: June 2021

Abstract
The NAM, ATAF, and CUC (NAC) family of transcription factors plays several important roles in plants, helping to regulate plant growth, development, senescence, and the response to biotic and abiotic stressors. NAC proteins also act as molecular switches, modulating hormonal responses to stress.A novel coding sequence (1059 bp) was cloned from hexaploid triticale. The putative protein encoded by this sequence (352 amino acids) was more than 95% similar to the amino acid sequence of a NAC protein from Aegilopsis tauschii (goatgrass; XP020161331), and phylogenetic analysis indicated that the novel gene formed a clade with goatgrass, Triticum turgidum , and barley. The novel protein contained a conserved nature actomyosin (NAM) domain (129 consecutive amino acids) between the 20 th and 148 th amino acids at the N-terminus and three transcriptional activation regions at the C-terminus. TwNAC01 was localized to the nucleus. Based on this evidence, the novel gene was identified as a triticale NAC gene and designated TwNAC01 (GenBank accession no. MG736919). After exposure to drought, Macrogol 6000 (PEG6000), NaCl, cold, methyl jasmonate (MeJA), and abscisic acid (ABA), TwNAC01 expression levels were greatest in triticale roots, followed by leaves and stems. Transgenic Arabidopsis thaliana overexpressing TwNAC01 had significantly lower leaf water loss rates and significantly longer roots than wild-type A. thaliana. Virus-induced silencing of the TwNAC01 gene in triticale delayed root development and decreased taproot length. Under drought stress, leaves of TwNAC01- silenced triticale had higher levels of malondialdehyde (MDA) and hydrogen peroxide (H 2 O 2 ) than the leaves of the wild type (WT), as well as lower relative water content (RWC), net photosynthetic rate, stomatal conductance, intercellular CO 2 concentration, and transpiration rate.Gene overexpression and silencing experiments suggested that TwNAC01 improves plant stress tolerance by increasing taproot length, regulating the water content of the plant leaves, reducing MAD and H 2 O 2 content, and adjusting respiration rate.
Optimization of the Maintenance Factors in Ampel Mosque, Surabaya, Indonesia
Authors: Sedayu Agung 
Published: June 2021

Abstract
The Ampel Mosque in Surabaya is an important historical site for Muslims in Indonesia. The architecture of the Ampel mosque is strongly influenced by Javanese and Arabic culture. a very harmonious integration between Javanese architecture as a form of authenticity and local wisdom in buildings, while Arabic architecture as a complementary and additional component. This research was, therefore, conducted to optimize the maintenance factor in the mosque building to ensure it functions properly and sustainably towards serving as a historic worship place for future generations. Data were collected using questionnaires distributed to the congregants or users of the mosques and analyzed through optimization using a dynamic program based on the level of importance. The results showed seven maintenance factors including the complexity of construction and design, serviceability, building durability, security and safety, aesthetics of the building, user comfort, and maintenance capability. Moreover, the optimization process prioritized the ease in construction work, stability of the construction, durability against structural damage to the building, use of non-hazardous materials, aesthetics of the building, the comfort of indoor and outdoor spaces, and ease of maintenance. These are expected to be the focus of the mosque manager in maintaining the authenticity of the building components. This, therefore, means the Ampel Mosque building can be maintained as a religious tourism destination at the national and international levels in order to ensure its historical value is sustained while the local and national economy is improved.
Seroprevalence of Bovine Brucellosis and its Public Health Significance in Central High Land of Ethiopia
Authors: Getahun Temesgen Kassa , Mamo Gezahegn , Urge Beksisa 
Published: June 2021

Abstract
Background: Brucellosis is a serious zoonotic disease affecting human and all domestic animals. It is considered to be one of the great public health problems all over the world. Little attention has been paid to bovine brucellosis in small holder dairy farm and in animal owners and farm workers in central highland of Ethiopia. Result: The overall seroprevalence of bovine brucellosis were 1.2% (95%CI: 0.47-2.97) by RBPT alone and 0.6% (95%CI: 0.016-2.09) using combined RBPT and CFT tests. In human, the overall seroprevalence of zoonotic brucellosis was 4.2% (95%CI: 0.204-8.42) by RBPT and 1.2% (95%CI: 0.032-4.27) using combined RBPT and CFT tests. Statistical analysis of risk factors by univariable firth’s bias reduced logistic regression indicates that late stage of abortion (OR=14.74, p=0.0002), retained fetal membrane (OR=32.74, p=0.006), market based stock replacement (OR=16.55, p=0.002), and presence of parturition pen (OR=11.511, p=0.027) were found to be significantly associated with the seropositivity for Brucella infection in dairy cattle. The seropositivity of human brucellosis was significantly influenced by human housing (OR=1.8, p=0.002), contact with aborted fetus (OR=21.19, p=0.017), drinking raw milk from aborted (OR=5.72, 0.019) and retained fetal membrane (OR=4.22, p=0.029) cows. A structured interview question was administered to 284 respondents. Accordingly, most respondents had no knowledge on brucellosis (93.3%). Conclusion: The present seroprevalence study revealed that brucellosis is prevalent at lower rate among dairy cattle and in exposed individuals in the study areas. However, since there were no control strategies implemented in the area, there is a potential risk for transmission of brucellosis in dairy cattle and exposed human population of study areas. With the low prevalence of bovine brucellosis in government owned and small holder farms, implementation of test and slaughter strategy with compensation to farmers is recommended; while in case of human brucellosis, continuous social training with feedback assessments, implementing one health approach framework and since its presence is confirmed and the risk factors are identified, the medical personnel should give attention as to differential diagnosis of the disease which has been overlooked so far.
Sensitivity of western north Pacific summertime tropical synoptic- scale disturbances to extratropical forcing – A regional climate model study
Authors: Liu Ying Lung , Tam Chi-Yung , Au-Yeung Andie Yee Man 
Published: June 2021

Abstract
The role of extratropical forcing on the summertime tropical synoptic-scale disturbances (TSDs) in the western north Pacific has been investigated, by conducting parallel integrations of the Regional Climate Model (RegCM). The suite of experiments consists of a control run (CTRL) with European Centre for Medium Range Forecasts (ECMWF) Reanalysis data as boundary conditions, and an experimental run (EXPT) with the same setting, except that signals with zonal wavenumber > 6 were suppressed at the northern boundary (located at 42°N) of the model domain. Comparison between CTRL and EXPT showed that, without extratropical forcing, there is weaker TSD activity in the June-to-August season, with reduced precipitation over the TSD pathway. Associated with suppressed TSD, southeastward-directed wave activity is also reduced, leading to less active mixed Rossby gravity (MRG) waves in the equatorial western Pacific area. Further analysis revealed that extratropical forcing and associated circulation changes can modulate the TSD wavetrain and its coherence structure, in relation to low-level vorticity in far western north Pacific. In CTRL, west of about 140°E, TSD-related circulation tends to be stronger; in EXPT, vorticity signals and vertical motions are found to be slightly more coherent in the more eastern portion of the TSD wavetrain. The latter enhanced coherency of TSD east of 140°E, from the EXPT simulations, might be due to changes in wave activity transport channelled by modulated upper-level mid-latitude westerlies in EXPT. Energetics indicate that changes in low-level background circulation itself can also influence TSD characteristics. Our results serve to quantify how extratropical forcing and related general circulation features influence western north Pacific summertime TSD activities. Implications on understanding the initiation of TSD, as well as their variability on longer time scales, are discussed.
Identification of Key Genes And Pathways Associated With Mast Cells Resting In Meningioma
Authors: Xie Hui , Yuan Ce , Ding Xiao-hui , Li Jin-jiang , Li Zhao-yang , Lu Wei-cheng 
Published: June 2021

Abstract
Background: To identify candidate key genes and pathways related to mast cells resting in meningioma and the underlying molecular mechanisms of meningioma. Methods: Gene expression profiles of GSE43290 and GSE16581 datasets were obtained from the Gene Expression Omnibus (GEO) database. GO and KEGG pathway enrichments of DEGs were analyzed using the ClusterProfiler package in R. The protein-protein interaction network (PPI), and TF-miRNA- mRNA co-expression networks were constructed. Further, the difference in immune infiltration was investigated using the CIBERSORT algorithm. Results: A total of 1499 DEGs were identified between tumor and normal controls. The analysis of the immune cell infiltration landscape showed that the probability of distribution of memory B cells, regulatory T cells (Tregs), and resting mast cells in tumor samples were significantly higher than those in the controls. Moreover, through WGCNA analysis, the module related to mast cells resting contained 158 DEGs, and KEGG pathway analysis revealed that the DEGs were dominant in the TNF signaling pathway, cytokine-cytokine receptor interaction, and IL-17 signaling pathway. Survival analysis of hub genes related to mast cells resting showed that the risk model was constructed based on 9 key genes. The TF-miRNA- mRNA co-regulation network, including MYC-miR-145-5p, TNFAIP3-miR-29c-3p, and TNFAIP3-hsa-miR-335-3p, were obtained. Further, 36 nodes and 197 interactions in the PPI network were identified. Conclusion: The results of this study revealed candidate key genes, miRNAs, and pathways related to mast cells resting involved in meningioma development, providing potential therapeutic targets for meningioma treatment.
Experimental measurement of thermal shock wave in C/Ph induced by electron beam radiation
Authors: Lin Yan-Jia , Lin Peng 
Published: June 2021

The attenuation characteristic of thermal shock wave in a new type of carbon phenolic (C/Ph) radiated by pulsed electron beam were studied experimentally on “FLASH II” accelerator with PVDF transducer. The attenuation trend of thermal shock wave produced by electron beam radiation in the material is calculated by means of numerical simulation. Experimental results show that: when electron beam energy fluxes are in the range of 169-531 J·cm-2 and the electron’s mean energy is about 0.6 MeV, stress at 4 mm from the irradiation surface of C/PH are in the range of 0.12-2.22GPa. The experimental results of the attenuation trend of thermal shock waves are in good agreement with the simulation results.
FEATURES OF INNOVATIVE POLICY OF CHINA. EXPERIENCE FOR RUSSIA
Authors: Moliy G. M. 
Published: June 2021

The article examines the features of China's economic and political reforms, which led to the country's transition to a new stage in the development of the national economy – a factor model based on investments. The absolute indicators demonstrating the level of scientific and technological development of China make the country one of the modern leaders. The country strives to become a leader in innovation by 2035. The paper notes the intermediate successes achieved by the Chinese government in the development of the economy of innovation. The country's interest in technologies of the 6th technological order is noted, expressed in the growth of investment in venture capital. The crisis caused by COVID-19 has had an impact on the global innovation sphere, and interest in developments in the field of medicine and healthcare has been noted. Expenditures for research in pharmaceuticals and biotechnology can be predicted to rise. In Russia, at present, in general, the main systems of the innovation system have been formed, while they are not sufficiently coordinated. The difficulty lies in the introduction of innovative technologies into production. Relying on the scientific achievements of the USSR period, using the experience of China, Russia has the opportunity to introduce innovative technologies. The article assesses the prospect of applying the experience of China in the national innovation system of Russia. An assessment of the features of the Russian innovation system is given.
CURRENT STATE, TRENDS AND CHALLENGES OF CHINA’S DIGITAL TRADE
Authors: Geng Changjun 
Published: June 2021

The digitalization of economy has a significant impact on the economic sector and on other activities. In recent years, there has been a sharp increase in the digitalization of the economy in China, primarily in such an area as digital trade. China's digital trade has grown steadily in recent years, and its share in China's trade has also increased. This article proposes the term “digital trade” and presents its characteristics. Analyze the state of the China’s digital trade, the features and main trends in the development of digital trade in goods and services. In addition, put forward current problems in the development of digital trade in China. It is concluded that digital trade in China as a whole has a positive trend, but in the future, instead of digital trade in goods, digital trade in services will become a major driving force of China's digital trade. With the development of cloud computing, cloud outsourcing and offshore services will become a new sector of digital services trade in the coming years. It was noted that China's digital trade is a driver for innovative trade growth and has an active impact on China's trade and economic activity.
CURRENT TRENDS IN INTERNATIONAL MERGERS AND ACQUISITIONS OF PHARMACEUTICAL COMPANIES
Authors: Sinitsova Yana S. 
Published: June 2021

The article analyzes the results of mergers and acquisitions of global pharmaceutical companies and their impact on the global pharmaceutical industry. The relevance of this research is determined by the high significance of mergers and acquisitions of global pharmaceutical companies on the global pharmaceutical industry and market. A comprehensive approach is used to assess the impact of mergers and acquisitions of pharmaceutical companies on the global pharmaceutical industry. The article presents statistical data on the volume and number of venture investments in the pharmaceutical and biotechnological industries. The reasons for mergers and acquisitions by pharmaceutical companies, as well as the dynamics of the activity of such transactions, are determined. The geographical structure of stock transactions and the total number of mergers and acquisitions of pharmaceutical companies are presented, as well as examples of the largest mergers and acquisitions of pharmaceutical companies.