bluuebunny/crossref_metadata_2025_split huggingface dataset:
The novel pleuromutilin derivative 22–((4-((4-nitrophenyl)acetamido)phenyl)thio)deoxy pleuromutilin possesses robust anti-mycoplasma activity both in vitro and in vivo
Authors: Xia Xirui , Ji Xuan , Li Yaxi , Wang Yubo , Zhao Yue , Wang Wenxiang , Ding Huanzhong 
Published: December 2024

ObjectiveMycoplasmas are structurally simple pathogenic microorganisms that can cause a wide range of diseases in humans and animals and conventional antibiotic therapies of fluoroquinolones and tetracyclines are toxic to young children and young animals and macrolide resistance is increasing. In this context, new anti-mycoplasma antimicrobial agents need to be developed. 22–((4-((4-nitrophenyl)acetamido)phenyl)thio)deoxypleuromutilin (compound 16C) is a novel acetamine phenyl pleuromutilin derivative. This study aimed to evaluate its acute toxicity in mice and generate pharmacokinetic and anti-mycoplasma profiles.MethodsThe safety of compound 16C was preliminarily evaluated by oral and intramuscular acute toxicity tests and single intravenous and intramuscular pharmacokinetic experiments were performed to obtain its pharmacokinetic profile. The minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), and time-killing curves reflected the in vitro effects of the compounds against Mycoplasma pneumoniae. Five groups consisted of three treatments for compound 16C (20, 40, and 80 mg/kg), and two treatments for tiamulin (oral and intramuscular 40 mg/kg) were continued for 4 d. Bronchoalveolar lavage fluid (BALF) and lung tissues were collected at the end of treatment (96 h) and 4 days later (192 h) to assess the in vivo anti-mycoplasma and anti-pneumonia effects. ELISA assays were performed to detect IFN-γ, TNF-α, and IL-8 (CXCL1) in BALF. Lung tissues were fixed with 4% paraformaldehyde and sectioned for histopathological assessment.ResultsThe results show that compound 16C has low toxicity (LD50 &gt; 5,000 mg/kg). Its pharmacokinetic profile is characterized by a short time to maximum concentration (Tmax = 0.24 h), high bioavailability (F = 71.29%), and short elimination half-life (T1/2kel) (intramuscular and intravenous administration was 2.20 and 1.89 h, respectively). Treatment with compound 16C and intramuscular tiamulin reduced the mycoplasma load in mice. Intramuscular compound 16C and tiamulin also inhibited the release of IFN-γ, TNF-α, and CXCL1, decreasing the accumulation of inflammatory cells in the lungs, thereby mitigating lung damage.ConclusionThis study proved that compound 16C has a strong antimicrobial effect against M. pneumoniae, can be rapidly absorbed and has therapeutic efficacy that provides a basis for developing new anti-mycoplasma drugs.
Tenecteplase: biochemical and clot lysis activity comparisons
Authors: Bechmann Jan , Schmid Ira , Brand Simone , Miller Felix , Zhang Chengzhi 
Published: December 2024

IntroductionIn the last decades, the recombinant tissue plasminogen activator alteplase has been the standard fibrinolytic treatment of acute myocardial infarction, pulmonary embolism, and acute ischemic stroke. An optimized version of alteplase, tenecteplase, has been developed by exchanging six amino acids to increase half-life, achieve higher fibrin selectivity and increase resistance to plasminogen activator inhibitor-1. Meanwhile, several products containing tenecteplase exist. The aim of this study was to compare the fibrinolytic activity and overall product quality of the 25 mg/vial presentation of tenecteplase originator Metalyse® (Boehringer Ingelheim Pharma GmbH and Co., KG, Ingelheim, Germany) to the 16 mg/vial formulation of the tenecteplase copy Mingfule® (CSPC Recomgen Pharmaceutical, Guangzhou, Co., Ltd.).MethodsWe have systematically analyzed and evaluated the biochemical and fibrinolytic differences between Metalyse® and Mingfule® using a wide range of routine quality testing assays, supplemented by mass spectrometry analysis and surface plasmon resonance assays. Additional host cell protein quantification and clot lysis testing following plasmin incubation over time were performed.ResultsSeveral key differences in biochemical composition and clot lysis activity were observed between the two tenecteplase variants. Versus Metalyse®, Mingfule® exhibited lower clot lysis activity and contained less of the two-chain form of tenecteplase. In addition, there were differences in sialic acid content, galactosylation, and fucosylation patterns, with Mingfule® exhibiting more bi- and less tri- and tetra-antennary glycosylation, leading to a different charge and size heterogeneity profile. Furthermore, Mingfule® displayed highly dissimilar binding to the three clearance receptors (LRP-1, ASGR, and mannose receptor) compared with Metalyse®. Purity analysis showed that Mingfule® contained a lower monomer content and, in contrast to Metalyse®, substantial amounts of host cell protein.DiscussionTaken together, these data demonstrate that the tenecteplase copy Mingfule® has several meaningful fibrinolytic and biochemical differences compared with Metalyse®. This raises the question of whether data from clinical studies with one of the products can be generalized for all tenecteplase variants.
Multimodal MRI radiomics-based stacking ensemble learning model with automatic segmentation for prognostic prediction of HIFU ablation of uterine fibroids: a multicenter study
Authors: Wen Bing , Li Chengwei , Cai Qiuyi , Shen Dan , Bu Xinyi , Zhou Fuqiang 
Published: December 2024

ObjectivesTo evaluate the effectiveness of an MRI radiomics stacking ensemble learning model, which combines T2-weighted imaging (T2WI) and contrast-enhanced T1-weighted imaging (CE-T1WI) with deep learning-based automatic segmentation, for preoperative prediction of the prognosis of high-intensity focused ultrasound (HIFU) ablation of uterine fibroids.MethodsThis retrospective study collected data from 360 patients with uterine fibroids who underwent HIFU treatment. The dataset was sourced from Center A (training set: N = 240; internal test set: N = 60) and Center B (external test set: N = 60). Patients were categorized into favorable and unfavorable prognosis groups based on the post-treatment non-perfused volume ratio. Automated segmentation of uterine fibroids was performed using a V-net deep learning models. Radiomics features were extracted from T2WI and CE-T1WI, followed by data preprocessing including normalization and scaling. Feature selection was performed using t-test, Pearson correlation, and LASSO to identify the most predictive features for preoperative prognosis Support Vector Machine (SVM), Random Forest (RF), Light Gradient Boosting Machine (LightGBM), and Multilayer Perceptron (MLP) were employed as base learners to construct base predictive models. These models were integrated into a stacking ensemble model, with Logistic Regression serving as the meta-learner to combine the outputs of the base models. The performance of the models was assessed using the area under the receiver operating characteristic curve (AUC).ResultsAmong the base models developed using T2WI and CE-T1WI features, the MLP model exhibited superior performance, achieving an AUC of 0.858 (95% CI: 0.756–0.959) in the internal test set and 0.828 (95% CI: 0.726–0.930) in the external test set. It was followed by the SVM, LightGBM, and RF, which obtained AUC values of 0.841 (95% CI: 0.737–0.946), 0.823 (95% CI: 0.711–0.934), and 0.750 (95% CI: 0.619–0.881), respectively. The stacking ensemble learning model, which integrated these five algorithms, demonstrated a notable enhancement in performance, with an AUC of 0.897 (95% CI: 0.818–0.977) in the internal test set and 0.854 (95% CI: 0.759–0.948) in the external test set.ConclusionThe DL based automatic segmentation MRI radiomics stacking ensemble learning model demonstrated high accuracy in predicting the prognosis of HIFU ablation of uterine fibroids.
Relay intercropped soybean promotes nodules development and nitrogen fixation by root exudates deposition
Authors: Lin Ping , Wang Jin , Chen Ping , Fu Zhidan , Luo Kai , Li Yiling , Pu Tian , Wang Xiaochun , Yong Taiwen , Yang Wenyu 
Published: December 2024

BackgroundLegumes, in the initial event of symbiosis, secrete flavonoids into the rhizosphere to attract rhizobia. This study was conducted to investigate the relationship between crop root exudates and soybean nodule development under intercropping patterns.MethodA two years field experiments was carried out and combined with pot experiments to quantify the effects of planting mode, i.e., relay intercropping and monocropping, and genotypes, i.e., supernodulating NTS1007(NTS), Nandou-12(ND) and Guixia-3(GX) on root exudates, rhizobium community structure, nodule development and nitrogen fixation ability.ResultThe result demonstrated that, maize–soybean relay strip intercropping not only promoted daidzein and genistein exudates of soybean root to soil but also reshaped the community structure and diversity of nodule endophytic rhizobia. Compared with monocropping, the nodule number significantly decreased in relay strip intercropping soybean, and NTS achieved 97% at soybean five trifoliolate stage. At soybean full bloom stage, despite the nodulation capacity of relay strip intercropping soybean was unrestored, the nodule number, nodule dry weight, nodule diameter and root dry weight were the highest in ND under relay strip intercropping. Compared with monocropping, the nodule average diameters of ND and GX in relay strip intercropping significantly increased 26.30% and 21.11%, respectively, the single nodule nitrogenase activity and nifH gene was increased up to the higher level of 3.16-fold and 1.96-fold, 70.8% and 107.6%, respectively. Combined with pot experiments, the nodule number of ND and NTS in maize root maize root exudates (RE) treatment increased with growth period, the GX reached its maximum at full bloom stage. And the nodule diameter of ND under RE treatment showed the best response. At R2 stage, compared with distilled water (DW) treatment, the nodule average diameter of ND and GX in RE treatment was significantly higher, and the GmEXPB2 gene was significantly up-regulated 3.99-fold and 1.02-fold, respectively.ConclusionIn brief, the maize–soybean relay strip intercropping enhanced the soybean root exudates nodulation signaling molecules, meanwhile, maize root exudates caused increased nodule diameter, and enhanced nodule nitrogen fixation, but had little effect on supernodulation varieties.
Rapid and accurate detection of Fusarium oxysporum f. sp. Lycopersici using one-pot, one-step LAMP-CRISPR/Cas12b method
Authors: Dai Shijie , Wu Yangsheng , Zhu Na , Zhao Yujin , Mao Mingjiang , Li Zheming , Zhu Bo , Zhao Weichun , Yuan Xiaofeng 
Published: December 2024

IntroductionFusarium oxysporum f. sp. Lycopersici (Fol) is one of the most devastating plant pathogenic fungi, the causal agent of root rot for Atractylides macrocephala Koidz (AMK). An accurate rapid and convenient diagnosis for FoL detection is essential for determining management practices and preventing future losses for AMK.MethodsHere, we developed a novel method for Fol detection by integrating loop-mediated isothermal amplification (LAMP) assay and CRISPR/Cas12b detection in one-pot, and the whole reaction can simultaneously amplify and detect the target gene of Fol in one-step.ResultsThe total time of the present method is limited to 45 min and isothermally performed at 60°C. The limit of detection of this assay is 88.9 copies per reaction. The specificity of the LAMP-CRISPR/Cas12b method was 100% without any cross-reaction of other pathogens. A total of 24 nucleic acid samples were used to evaluate the performance of the LAMP-CRISPR/Cas12b method, including 12 with-Fol and 12 without-Fol. Compared with the gold standard results from real-time PCR, the present method provides a sensitivity of 100% (12/12), specificity of 100% (12/12), and consistency of 100% (24/24).DiscussionTogether, our preliminary results illustrated that the LAMP-CRISPR/Cas12b method is a rapid simple, and reliable tool for Fol diagnosis and could be applied in point-of-need phytopathogen detection.
Improvement of antioxidant capacity, aroma quality, and antifungal ability of cherry by phenyllactic acid treatment during low temperature storage
Authors: Zhang Chaoqi , Wang Yunfan , Wang Mengxin , Kong Yanhui , Li Xiulian , Song Danliangmin , Zeng Xiangquan , Yang Yanqing , Fan Xinguang , Gong Hansheng 
Published: December 2024

IntroductionSweet cherries (Prunus avium L.) are highly valued for their taste and nutrients but are prone to decay due to their delicate skin and high respiration rate. Traditional chemical preservatives have drawbacks like residues and resistance, prompting the search for natural alternatives. Phenylactic acid (PLA) has shown promise due to its antibacterial and antioxidant properties, making it a potential natural preservative to extend cherry shelf life.Methods'Stella' sweet cherries were treated with varying concentrations of PLA (0, 2, 4, 8, 16 mmol·L-1) and stored at 4°C. Key quality indicators, including firmness, total acidity, total soluble solids, weight loss, decay index, and antioxidant activity, were assessed over time. Additionally, HPLC, GC-MS, GC-IMS, colony counts, in vivo inhibition analyses were conducted to evaluate phenolic content, aroma compounds and antifungal ability.Results and DiscussionPLA at 8 mmol·L-1 effectively maintained cherry quality by reducing weight loss and decay of cherries, delaying the decline of firmness, while enhancing antioxidant capacity, flavor stability, and antifungal ability. Higher concentrations (16 mmol·L-1) provided stronger antimicrobial effects but caused slight surface wrinkling. Thus, 8 mmol·L-1 was optimal, balancing preservation and appearance, making it a promising natural preservative for extending cherry shelf life.
The experiences of consumers, clinicians and support persons involved in the safety planning intervention for suicide prevention: a qualitative systematic review and meta-synthesis
Authors: O’Connor Edward , Rhodes Kate , Procter Nicholas , Loughhead Mark , Procter Alexandra , Reilly Julie-Anne , Pettit Sophie , Ferguson Monika 
Published: December 2024

BackgroundThe Safety Planning Intervention (SPI) is an efficacious brief intervention for supporting people experiencing suicidal ideation and behavior. However, the subjective experiences of those who have used the SPI have not been systematically evaluated. This systematic review synthesized qualitative evidence regarding the experiences of people involved in the SPI.MethodSystematic searches of international, peer-reviewed, English language literature were conducted in seven databases (CINAHL, Embase, Emcare, MEDLINE, PsycInfo, Scopus and Web of Science).ResultsA total of 588 articles were screened for eligibility, with screening, data extraction, and critical appraisal conducted in duplicate. Qualitative data were extracted from 10 included studies and synthesized via meta-aggregation. Ninety individual findings were aggregated into 14 unique categories, with categories subsequently combined to produce four synthesized findings: acceptability and positive outcomes associated with the SPI; maximizing the effectiveness of the SPI; navigating the involvement of support persons in the SPI process; barriers and limitations associated with the SPI.DiscussionCollectively, findings indicate that the SPI is viewed as beneficial by users and can be enhanced through clinicians’ use of a person-centered, collaborative approach, as well as through the inclusion of support persons. Future research should seek lived experience understandings from more diverse stakeholders, particularly regarding consumers’ experiences of using the SPI during acute distress. Further research is required to investigate causal pathways between SPI engagement and improved outcomes.Systematic review registrationhttps://www.crd.york.ac.uk/prospero/display_record.php?ID=CRD42022312425, identifier CRD42022312425.
Do we interpret ambiguity and feel according to how we define ourselves? Relationships between self-perception, interpretation biases, and their role on emotional symptoms
Authors: Martin-Garcia Oscar , Blanco Ivan , Sanchez-Lopez Alvaro 
Published: December 2024

IntroductionIn today's fast-paced world, depression and anxiety are the most prevalent health problems, generating high economic and social burdens. Interpretation biases seem to play a pivotal role in this emotional problems, influencing how individuals interpret emotionally ambiguous information. These interpretation biases can emerge due to the activation of latent schemas related to how individuals perceive themselves. Therefore, integrating the study of cognitive and self-discrepancy models can offer a comprehensive approach to better understanding the onset or maintenance of emotional symptoms, through their relationship with interpretation biases. In this paper, we aimed to test whether differences in self-perception might act like a cognitive schema that activate cognitive bias, influencing information processing and predicting emotional symptoms.MethodSeventy-three undergraduates completed two different experimental tasks, evaluating self-discrepancies and self-referential negative interpretation bias. Moreover, emotional symptoms were collected after completing the tasks and 1-2 months after, prior to coping with a natural stressor (exam period). The main analyses comprised mediational models, both cross-sectional and longitudinal, with the aim to test whether interpretation bias might act like a mediator in the relation between self-discrepancies and emotional symptoms.ResultsFirst, the results showed significant correlations between higher levels of self-discrepancies (actual-ideal and actual-ought) and higher levels of emotional symptoms (depression and anxiety), as well as with higher negative interpretation biases. Further, cross-sectional mediational models showed that negative interpretation biases partially mediated the relationship between self-discrepancies and emotional symptoms. As for the longitudinal mediation analysis, interpretation bias only mediated the specific relation between actual-ideal self-discrepancies and increases in anxiety symptoms, while the rest of the indirect effects were not significant.DiscussionThese results suggest that self-discrepancies could be understood as indices of the activation of latent cognitive schemas, which would influence subsequent stages of information processing, such as negative interpretations of ambiguous information, partly accounting for the emergence and/or maintenance of emotional symptoms.
Behind the good of digital tools for occupational safety and health: a scoping review of ethical issues surrounding the use of the internet of things
Authors: El Bouchikhi Maéva , Weerts Sophie , Clavien Christine 
Published: December 2024

IntroductionThe internet of things (IoT) is increasingly used for occupational safety and health (OSH) purposes in private and public organisations. Current practices and regulations are unclear, and some stakeholders raised concerns about deploying this technology at work.MethodsFollowing the PRISMA-ScR checklist, we reviewed the main opportunities and ethical issues raised by using IoT devices for OSH purposes, as discussed in the academic literature. We searched peer-reviewed papers from 2008 to September 2023, written in English and available in “Web of Science,” “PhilPapers,” and “Google Scholar.” We found 1,495 articles, of which 61 fulfilled the selection criteria. We classified ethical topics discussed in the papers in a coherent description frame.ResultsWe obtained 6 overarching categories: “ethical opportunities,” “surveillance and problematic data re-purposing,” “difficulty to inform, consult, and obtain consent from employees,” “unintended and unpredictable adverse effects,” “suboptimal data management,” and “external factors that are conducive to ethical issues.” The resulting list of problematic issues is unexpectedly furnished and substantial. Such a list provides information and guidance for those who wish to develop evaluation frameworks in line with a preventive regulatory approach. It also informs policymakers and practitioners about the governance of such tools for ensuring more OSH.
The increasing incidence and mortality of bacteremia in Denmark from 2010 to 2022: a population-based nationwide cohort study
Authors: Dessau Ram B. , Andersen Christian Østergaard , Coia John , Ellermann-Eriksen Svend , Gubbels Sophie , Jensen Thøger Gorm , Knudsen Jenny Dahl , Kähler Jonas , Lomborg Steen , Lützen Lisbeth , Nielsen Marc Trunjer Kusk , Olesen Bente Scharvik , Pinholt Mette , Scheutz Flemming , Søgaard Kirstine Kobberøe , Voldstedlund Marianne , Mølbak Kåre 
Published: December 2024

ObjectivesAn increasing incidence of positive blood cultures has been reported in several countries. This represents an important challenge for public health due to the large number of hospital admissions with high mortality among these patients. This study aims to characterize the epidemiology and mortality associated with positive blood cultures.Study design and methodsWe performed a nationwide cohort study, identifying all individuals with a blood culture drawn in Denmark from 2010 to 2022. We estimated the incidence of positive blood cultures and 30-day mortality per 100,000 individuals and modeled the case fatality rate (CFR) and population incidences with regression techniques.ResultsWe identified almost 4 million blood cultures drawn during the study period, increasing 64% from 220,757 in 2010 to 362,239 in 2022. During this period, the underlying population increased only 10%, whereas the population of older adults above 85 years increased 3.3 times. The positivity rate was 9.8%, and the most common pathogens were Escherichia coli (29%) and other pathogens associated with urinary tract infection, whereas Staphylococcus aureus accounted for 12%, and Enterococcus faecium for 4.1%. The 30-day CFR was 17% with a small reduction during the study period, with notable differences according to species.ConclusionWe found a substantial rise in blood culture activity and the incidence of positive cultures. This rise is driven by an increasing number of older citizens, which is expected to increase further in the coming years. Considering the high risk of mortality and increasing incidence, a national surveillance of bacteremia is warranted.