bluuebunny/crossref_metadata_2025_split huggingface dataset:
Ammonotely in a passerine nectarivore: the influence of renal and post-renal modification on nitrogenous waste product excretion
Authors: Roxburgh Lizanne , Pinshow Berry 
Published: April 2021

SUMMARYMost aquatic vertebrates are ammonotelic, whereas terrestrial vertebrates are typically uricotelic or ureotelic. However, the principal form of nitrogenous waste product in the urine of an animal may vary, depending on environmental conditions. Anna's hummingbird (Calypte anna) was found to switch from uricotely at high ambient temperature (Ta)to ammonotely at lower Ta, when energy demands and consequent nectar intake rates were high. In extension of this, we hypothesised that nectarivorous birds would switch from uricotely to ammonotely when water intake rates were high or when protein or salt intake rates were low. We examined the influence of water, electrolyte and protein intake and of Ta on the excretion of ammonia, urea and urate (uric acid and its salts) in nectarivorous Palestine sunbirds(Nectarinia osea). The proportion of ammonia in ureteral urine and excreted fluid was not influenced by total water or salt intake or by Ta. Protein intake did not influence nitrogenous waste product concentrations in ureteral urine. However, when protein intake was reduced, the proportion of ammonia in excreted fluid was higher because of the reduced urate concentration. This reduction in urate concentration leads to`apparent' ammonotely. We suggest that ammonotely may not be a unique feature of nectarivorous birds. It could occur in any species in which breakdown of urate in the hindgut allows the uric acid-nitrogen concentration in the excreta to fall below that of the ammonia-nitrogen concentration.
Osmotic effects on arginine kinase function in living muscle of the blue crab Callinectes sapidus
Authors: Holt Stewart M. , Kinsey Stephen T. 
Published: April 2021

SUMMARY
Flux was examined through the reaction catalyzed by arginine kinase in intact blue crab (Callinectes sapidus) muscle during simulated changes in salinity. Isolated dark levator muscles from the swimming leg were superfused with a saline solution that had an osmolarity equivalent to that of the hemolymph under different salinity regimes. Animals were acclimated for 7 days to a salinity of 5, 17 or 35‰, which corresponds to a hemolymph osmolarity of 640, 720 or 960 mosmoll-1, respectively. Experiments were conducted under control conditions, in which the osmolarity of the superfusion medium matched that of the acclimated hemolymph, as well as under hypo- and hyperosmotic conditions. These latter treatments were meant to simulate a rapid change in environmental salinity. Pseudo-first-order unidirectional rate constants and flux rates were measured for arginine kinase in the forward and reverse directions using a 31P-nuclear magnetic resonance saturation transfer method. There were no differences in the rate constants or flux rates among the controls, indicating that arginine kinase function is not modulated by salinity if the animal has had sufficient acclimation time. However, the rate constants and flux rates of arginine kinase varied over a modest 1.7-fold range across the three types of osmotic treatments, although the range for the flux data was reduced when cell volume changes were taken into account. The hyperosmotic treatments led to a reduction in arginine kinase flux, while the hypo-osmotic treatments led to an enhanced arginine kinase flux. We propose that this effect is mediated by an increase in the concentration of perturbing inorganic ions under hyperosmotic conditions and a decrease in the concentration of such ions during the hypo-osmotic treatments.
Fast muscle in squid (Loligo pealei): contractile properties of a specialized muscle fibre type
Authors: Kier William M. , Curtin Nancy A. 
Published: April 2021

SUMMARYThe contractile properties of the transverse muscle of the tentacles and the transverse muscle of the arms of the squid Loligo pealei were investigated using small muscle fibre bundle preparations. In addition,transmission electron microscopy was used to measure the length of the thick myofilaments of the two muscle fibre types. The thick filament length of the cross-striated tentacle fibres was 0.81±0.08 μm (mean ± S.D, N=51) while that of the obliquely striated arm muscle fibres was 7.41±0.44 μm (N=58). The difference in thick filament length of the two muscle types was predicted to result in a much higher shortening velocity of the tentacle muscle compared with the arm muscle. This was tested by investigating the force/velocity relationship for isotonic shortening of the two muscle types. Fitting Hill's equation to the results gave a maximum shortening velocity (Vmax, the intercept on the velocity axis) of 15.4±1.0 L0 s-1(mean ± S.D., N=9) for the tentacle fibres and of 1.5±0.2 L0 s-1 (N=8) for the arm fibres, where L0 is the length at which peak isometric force was recorded. The difference in thick filament length was also predicted to result in lower peak tension in the tentacle versus the arm muscle. For the tentacle, the mean peak tetanic tension during a brief isometric tetanus (0.2s) of 131±56 mN mm-2 cross-sectional area (mean ± S.D., N=12) was observed at a stimulus frequency of 80 Hz, whereas the mean peak tetanic tension of the arm fibres during a brief isometric tetanus (0.2s) was 468±91 mN mm-2(N=5) and was observed at a stimulus frequency of 160 Hz. The length/force relationships (expressed relative to L0) of the two muscle types were similar. The ratio of twitch force to peak tetanic force was 0.66 in the tentacle fibres, but only 0.03 in the arm fibres.
Analysis of Ca2+ uptake into the smooth endoplasmic reticulum of permeabilised sternal epithelial cells during the moulting cycle of the terrestrial isopodPorcellio scaber
Authors: Hagedorn Monica , Ziegler Andreas 
Published: April 2021

SUMMARYIn terrestrial isopods, large amounts of Ca2+ are transported across anterior sternal epithelial cells during moult-related deposition and resorption of CaCO3 deposits. Because of its toxicity and function as a second messenger, resting cytosolic Ca2+ levels must be maintained below critical concentrations during epithelial Ca2+transport, raising the possibility that organelles play a role during Ca2+ transit. We therefore studied the uptake of Ca2+into Ca2+-sequestering organelles by monitoring the formation of birefringent calcium oxalate crystals in permeabilised anterior and posterior sternal epithelium cells of Porcellio scaber during Ca2+-transporting and non-transporting stages of the moulting cycle using polarised-light microscopy. The results indicate ATP-dependent uptake of Ca2+ into organelles. Half-maximal crystal growth at a Ca2+ activity, aCa, of 0.4 μmol l-1 and blockade by cyclopiazonic acid suggest Ca2+uptake into the smooth endoplasmic reticulum by the smooth endoplasmic reticulum Ca2+-ATPase. Analytical electron microscopical techniques support this interpretation by revealing the accumulation of Ca2+-containing crystals in smooth membranous intracellular compartments. A comparison of different moulting stages demonstrated a virtual lack of crystal formation in the early premoult stage and a significant fivefold increase between mid premoult and the Ca2+-transporting stages of late premoult and intramoult. These results suggest a contribution of the smooth endoplasmic reticulum as a transient Ca2+ store during intracellular Ca2+ transit.
S- and C-start escape responses of the muskellunge (Esox masquinongy) require alternative neuromotor mechanisms
Authors: Hale Melina E. 
Published: April 2021

SUMMARYThe startle response is a model system for examining the neural basis of behavior because of its relatively simple neural circuit organization and kinematic pattern. In fishes, the two primary types of startle behavior differ in their initial movements. In the C-start type of startle, the fish bends into a C shape, while the S-start involves an S-shaped body bend. Although considerable research has focused on determining how the C-start is generated neurally, S-start neurobiology has not been examined. I quantify the kinematics and electromyographic patterns of the initial movements of the C-start and S-start behaviors of the muskellunge (Esox masquinongy)to test three hypotheses for how the S-start is generated. (i) The S-start is generated by the same motor neural circuit as the C-start, but passive bending of the tail causes the body to take on an S shape. (ii) The S-start is generated by the same motor neural circuit as undulatory swimming. (iii) The S-start is generated by an independent neural mechanism from that used either in the C-start or in undulatory swimming. Results from kinematics and muscle activity patterns support the third hypothesis. In the muskellunge, the S-start is a high-performance startle behavior with peak angular velocity and peak angular acceleration of its initial bending comparable with those of the C-start and higher than would be expected for undulatory swimming. The S-start motor pattern, however, is distinct from the C-start motor pattern in having simultaneous muscle activity anteriorly on one side of the body and posteriorly on the opposite side. In contrast, the C-start is characterized by simultaneous unilateral muscle activity along the full length of the body. Alternative models are proposed for S-start neural circuit organization involving reticulospinal and local control of muscle activity.
Spectral reflectance and directional properties of structural coloration in bird plumage
Authors: Osorio D. , Ham A. D. 
Published: April 2021

SUMMARY
Bird plumage is coloured both by pigments and by spectrally selective interference in the light reflected from feather barbs. These barbs are composites of high- and low-refractive-index materials, and light is reflected at refractive index boundaries. The spatial structure determines the wavelengths where constructive interference occurs and, hence, the spectral tuning. The spectral tuning of interference colours often varies with angles of illumination and reflection, which produces iridescence. Iridescence and other optical effects mean that interference coloration looks different from pigmentation and is visually striking. To study the optical and visual properties of structural plumage colours, we recorded the reflectance spectra of feathers and in particular their directional properties. A fixed spot on a feather was viewed from a fixed position, whilst the feather orientation and the angle of illumination were varied. We recognise two main types of coloration, `directional' and `diffuse'. Within these types, there is considerable variation, and five examples illustrate some features of structural plumage colours and suggest how their optical and visual properties can be measured and described.
Glutamine synthetase expression in liver, muscle, stomach and intestine ofBostrichthys sinensisin response to exposure to a high exogenous ammonia concentration
Authors: Anderson P. M. , Broderius M. A. , Fong K. C. , Tsui K. N. T. , Chew S. F. , Ip Y. K. 
Published: April 2021

SUMMARYA previous study provided evidence that the adaptive strategy used by the teleost fish Bostrichthys sinensis (sleeper) for detoxifying ammonia during extended periods of air exposure was to synthesize and store glutamine,primarily in the muscle, accompanied by an increase in glutamine synthetase(GSase) activity in liver. The aim of the present study was to assess the effect on GSase expression in various tissues of exposure of B. sinensis to exogenous ammonia. Exogenous ammonia increases internal ammonia concentrations in fish, mimicking environmental situations such as air exposure that preclude loss of ammonia across the gills, and thus triggering alternative mechanisms for ammonia detoxification. The results reveal relatively high levels of GSase activity, not only in liver but also,unexpectedly, in muscle, and even higher levels in intestine and, in particular, stomach. Exposure to ammonia results in significant increases in GSase activity, GSase protein and GSase mRNA levels in all of these tissues except stomach. The amino acid sequences of GSases from liver and stomach deduced from the cDNA sequences are essentially identical and are &gt;97 %identical to the amino acid sequences of GSases from Gulf toadfish(Opsanus beta) and marble goby (Oxyeleotris marmoratus).
Long-term fasting and realimentation in hypogean and epigean isopods: a proposed adaptive strategy for groundwater organisms
Authors: Hervant Frédéric , Renault David 
Published: April 2021

SUMMARYThe effects of long-term fasting and subsequent refeeding on digestive physiology and energy metabolism were investigated in a subterranean aquatic crustacean, Stenasellus virei, and in a morphologically similar surface-dwelling species, Asellus aquaticus. Metabolic response to food deprivation was monophasic in A. aquaticus, with an immediate,large decrease in all energy reserves. In contrast, S. vireidisplayed three successive periods of phosphageno-glucidic, lipidic and,finally, proteo-lipidic-dominant catabolism over the course of the nutritional stress. To represent the responses of subterranean crustaceans to food stress and renutrition, a sequential energy strategy was hypothesized, suggesting that four successive phases (called stress, transition, adaptation and recovery) can be distinguished. Based on these results, a general adaptive strategy for groundwater organisms was proposed. Their remarkable resistance to long-term fasting may be partly explained by (1) a depressed metabolism,during which they mainly subsist on lipid stores, (2) a prolonged state of glycogen- and protein-sparing, (3) low energetic requirements and (4) large body stores. In addition, these groundwater species displayed high recovery abilities during refeeding, showing an optimal utilization of available food and a rapid restoration of their body reserves. These adaptive responses might be considered for numerous subterranean organisms as an efficient energy-saving strategy in a harsh and unpredictable environment where fasting(and/or hypoxic) periods of variable duration alternate with sporadic feeding events (and/or normoxic periods). Therefore, food-limited and/or hypoxia-tolerant groundwater species appear to be good examples of animals representing a low-energy system.
In the polymorphic ciliateTetrahymena vorax, the non-selective phagocytosis seen in microstomes changes to a highly selective process in macrostomes
Authors: Grønlien Heidi K. , Berg Trond , Løvlie Arne M. 
Published: April 2021

SUMMARYCiliates use phagocytosis to acquire edible particles. The polymorphic ciliate Tetrahymena vorax appears in two forms (`microstomes' and`macrostomes'). Transformation of microstomes into macrostomes takes place in the presence of the ciliate Tetrahymena thermophila and enables the macrostome to phagocytose the latter species. The non-specific, constitutive phagocytosis in microstomes thereby changes into a specific inducible process in macrostomes. The purpose of this study was to determine whether the phagocytotic process in macrostomes is specifically aimed at catching T. thermophila. The two forms of phagocytosis represent an interesting model system for studying the mechanism whereby phagosomes are formed. The macrostomal form capture deciliated and ciliated Tetrahymena thermophila, latex beads with diameters of 20.3 and 30.0 μm and small microstomal cells. However, the macrostomes select T. thermophila as a prey when they have the opportunity to choose between deciliated T. thermophila and latex beads and between T. thermophila and microstomes. The non-selective formation of phagosomes seen in microstomes changes to a highly selective process during the transformation to macrostomes. Unlike microstomes, macrostomes do not form a closed vacuole after capturing a latex bead, indicating that mechanical stimulation by the prey does not in itself trigger phagocytosis in the macrostomal form of T. vorax. Although macrostomes captured T. thermophila in preference to microstomes, phagocytosis of microstomes started immediately following capture, indicating that the substance/molecule that triggers the formation of the phagosome is not specific for T. thermophilacells.After capturing a T. thermophila cell, the macrostomal cell, which normally swims in a forward direction, reverses direction and swims backwards for a short time before starting to rotate. Macrostomal cells did not change their swimming pattern after capturing a latex bead. We believe, therefore,that backward swimming is more likely to be related to signals resulting from phagocytosis than from mechanical stimulation of the pouch.Cytochalasin B (10 μg ml-1) inhibits phagocytosis in both microstomes and macrostomes, indicating that actin filaments play an active role in phagocytosis in both cell types. The antitubulin drug nocodazole(0.3-30 μmol l-1) inhibits the formation of more than one phagosome in the macrostome, indicating that membrane transport to the oral apparatus in macrostomes is guided by microtubules. Nocodazole has no effect on the process of phagocytosis in microstomes.
Effect of environmental temperature on body temperature and metabolic heat production in a heterothermic rodent,Spermophilus tereticaudus
Authors: Wooden K. Mark , Walsberg Glenn E. 
Published: April 2021

SUMMARYThis study quantifies the thermoregulatory ability and energetics of a mammal, the round-tailed ground squirrel Spermophilus tereticaudus,that can relax thermoregulatory limits without becoming inactive. We measured body temperature and metabolic rate in animals exposed for short periods (1 h)to air temperatures ranging from 10 to 45 °C and for long periods (8 h) to air temperatures ranging from 10 to 30 °C. Within 45 min of exposure to air temperatures ranging from 10 to 45 °C, the mean body temperatures of alert and responsive animals ranged from 32.1 °C(Tair=10 °C) to 40.4 °C(Tair=45 °C). This thermolability provided significant energetic savings below the thermoneutral zone, ranging from 0.63 W (18 %) at 10 °C to 0.43 W (43 %) at 30 °C. When exposed for 8 h to air temperatures between 10 and 30 °C, animals varied their body temperature significantly over time. At all air temperatures, the lowest body temperature(maintained for at least 1 h) was 31.2 °C. The highest body temperatures(maintained for at least 1 h) were 33.6 °C at 10 °C, 35.3 °C at 20°C and 36.3 °C at 30 °C. The energetic savings realized by maintaining the minimum rather than the maximum body temperature was 0.80 W(25 %) at 10 °C, 0.71 W (33 %) at 20 °C and 0.40 W (47 %) at 30°C. This study demonstrates in several ways the ability of this species to adjust energy expenditure through heterothermy.
Normal mammalian skeletal muscle and its phenotypic plasticity
Authors: Hoppeler Hans , Flück Martin 
Published: April 2021

SUMMARYSince muscle mass makes up such a high proportion of total body mass, there must have been considerable selective pressure to minimize the cost of maintenance and to maximize the functionality of muscle tissue for all species. Phenotypic plasticity of muscle tissue allows the species blueprint of muscle tissue to be modified to accommodate specific demands experienced by animals over their lifetime. In this review, we report the scaling of muscle structural compartments in a set of mammals spanning five orders of magnitude(17 g woodmice to 450 kg horses and steers). Muscle mass, muscle myofibrillar volume and sarcoplasmic space were found to represent similar relative quantities in all species studies (scaling factor close to unity). Mitochondrial volumes were found to be systematically smaller in larger animals (scaling factor 0.91) and closely related to the scaling of V̇O2max (0.92) and were tracked by the scaling of total capillary length (0.95). In this set of species, we therefore found that maximal metabolic rate and supporting structures did not scale to the 0.75 power of body mass as generally suggested. Muscle phenotypic plasticity is reasonably well characterized on a structural and functional basis, but we still know little about the signals that cause the changes in gene expression necessary for phenotypic changes in muscle. The molecular responses of human m. vastus lateralis to endurance exercise indicate that a single bout of exercise causes specific transient transcriptional adaptations that may gradually accumulate after their translation into the (structural) modifications seen with phenotypic plasticity. Metabolic and mechanical factors are recognized candidate factors for the control of exercise-induced gene transcription in muscle. Distinct protein kinases and transcription factors emerge as possible interfaces that integrate the mechanical (MAPKs and jun/fos) and metabolic (AMPK, HIF-1αand PPARα) stimuli into enhanced gene transcription in skeletal muscle.
Muscle designed for maximum short-term power output: quail flight muscle
Authors: Askew Graham N. , Marsh Richard L. 
Published: April 2021

SUMMARYTake-off in birds at high speeds and steep angles of elevation requires a high burst power output. The mean power output of the pectoralis muscle of blue-breasted quail (Coturnix chinensis) during take-off is approximately 400 W kg-1 muscle, as determined using two independent methods. This burst power output is much higher than has been measured in any other cyclically contracting muscle. The power output of muscle is determined by the interactions between the physiological properties of the muscle, the stimulation regime imposed by the central nervous system and the details of the strain cycle, which are determined by the reciprocal interaction between the muscle properties and the environmental load. The physiological adaptations that enable a high power output to be achieved are those that allow the muscle to develop high stresses whilst shortening rapidly. These characteristics include a high myofibrillar density, rapid twitch contraction kinetics and a high maximum intrinsic velocity of shortening. In addition, several features of the strain cycle increase the power output of the quail pectoralis muscle. First, the muscle operates at a mean length shorter than the plateau of the length/force relationship. Second,the muscle length trajectory is asymmetrical, with 70 % of the cycle spent shortening. The asymmetrical cycle is expected to increase the power output substantially. Third, subtle deviations in the velocity profile improve power output compared with a simple asymmetrical cycle with constant lengthening and shortening rates. The high burst power outputs found in the flight muscles of quail and similar birds are limited to very brief efforts before fatigue occurs. This strong but short flight performance is well-suited to the rapid-response anti-predation strategy of these birds that involves a short flight coupled with a subsequent sustained escape by running. These considerations serve as a reminder that the maximum power-producing capacities of muscles need to be considered in the context of the in vivosituation within which the muscles operate.