bluuebunny/crossref_metadata_2025_split huggingface dataset:
Self-Made Distal Femoral Extramedullary Positioning Osteotomy Template In Total Knee Arthroplasty
Authors: zhe Sun , hussein Dahir , Gang Zhao , Chaojun Sun , Qazi Tariq Siraj , kang yang , Zhifu Hu , Haque Md Ariful , Hong li , chang Zhao , Jing xu , Yue Li , Li Sun 
Published: September 2021

Abstract
Introduction: The objective of this study was to assess the success of total knee arthroplasty with the use of extramedullary positioning osteotomy templates for limb alignment in contrast to traditional intramedullary positioning rods. Methods: Between September 2019 and February 2021, 70 participants underwent total knee arthroplasty with lower limb positioning using an extramedullary distal femur positioning template and intramedullary positioning rods. The participants were divided into two groups each to receive either of the two limb positioning techniques. The groups were divided randomly and were similar at the beginning of the study. Therefore, the participants were equally divided into 35 participants per group. Participants' indication for the procedure was severe osteomyelitis or rheumatoid arthritis, and they underwent distal femoral osteotomy. The force lines of the lower limb were identified by locating the center of the head of the femur. The patients were monitored for intraoperative bleeding, surgical trauma and surgical time. They were followed up during the first and second weeks post-operation to assess for knee joint movement graded using the HSS knee score and assessed for deviation. Participants were treated equally throughout the study and during the follow-up phase. Results: The participants did not have any statistically significant differences at the beginning of the study. The intramedullary group had a lesser average age but without any significant difference from the extramedullary group. In addition, their HSS knee scores, body mass index, knee joint range of motion, and the angle of deviation of the knee, hip, and ankle were of no statistical significance with a p-value of more than 0.05. The operation time for the extramedullary group was approximately 96.57 minutes with a margin of error of (+/-) 8.576 minutes. In contrast, the intramedullary group had an average time of 104.8 minutes and a margin of error of (+/-) 8.864. Intraoperative bleeding for the extramedullary group was less at approximately 89.97 ml, while it was 95.4ml in the intramedullary group. Postoperative drainage was also more in the intramedullary group as compared to the intramedullary group. The differences between both groups were statistically significant, with a p-value of less than 0.05. There was no significant difference in HKA angle deviation, coronal plane angle, and flexion angle of femoral prosthesis (P > 0.05). Conclusion: Extramedullary distal femoral osteotomy positioning templates proved superior to intramedullary positioning rods. The template demonstrated less surgical trauma hence the lower risk of deformity, less intraoperative bleeding, therefore, more safety and less risk for infection than the intramedullary positioning rods.
Transcriptome and proteome dynamics during the embryonation of Fasciola hepatica eggs
Authors: Ilgová Jana , Vorel Jiří , Roudnický Pavel , Škorpíková Lucie , Horn Martin , Kašný Martin 
Published: September 2021

Abstract
Fasciola hepatica is a global parasite of livestock which also causes a neglected zoonosis in humans. The parasite’s communication with the host during its complicated lifecycle is based on an ingenious enzymatic apparatus which includes a variety of peptidases. These enzymes are implicated in parasite migration, pathogenesis of the disease, and modification of host immune response. Although the dynamics of proteolytic machinery produced by intra-mammalian F. hepatica life stages has been previously investigated in great detail, peptidases of the eggs so far received little scientific attention. In this study, we performed a comparative RNA-seq analysis aimed at identification of peptidases expressed in F. hepatica eggs at different ages and employed mass spectrometry in order to identify and quantify peptidases translated in F. hepatica egg lysates. Finally, we measured and classified proteolytic activities in extracts from F. hepatica eggs using a library of fluorogenic substrates and peptidase class-selective inhibitors. Using these methods, we observed significant changes in the composition of peptidases during egg maturation.
Application of Intravenous Vitamin C in Adult Patients with Sepsis: A Meta-Analysis of Randomized Controlled Trials
Authors: Chen Han-Bing , Chen Peng , Li Kang , Shao Jun 
Published: September 2021

Abstract
Background: The efficacy of intravenous vitamin C among sepsis patients is uncertain according to recent randomized controlled trials (RCTs). We conducted a meta-analysis to evaluate the efficacy of vitamin C application in adults with sepsis.Methods: We performed a systematic literature search in PubMed, Web of Science, Embase and the Cochrane Library. Eligible studies were RCTs that investigated the application of intravenous vitamin C in adult patients with sepsis. We assessed the risk of bias of the included studies using the Cochrane risk of bias tool and the certainty of evidence according to the Grading of Recommendations, Assessment, Development, and Evaluation (GRADE) approach, for each outcome.Results: Fourteen trials involving a total of 1823 patients were included. We found that there was no significant effect of vitamin C on 28-day mortality [risk ratio (RR) 0.87, 95% confidence interval (CI) 0.73 to 1.04, p = 0.12, TSA-adjusted CI 0.70 to 1.08, low quality evidence], but among patients who were treated with vitamin C monotherapy instead of combination therapy, the mortality was reduced (RR 0.66, 95% CI 0.49 to 0.88, p = 0.004). Vitamin C was associated with a significant improvement of 72-h ΔSOFA score (SMD = 0.20, 95% CI 0.07 to 0.32, p = 0.002, I2=11%, moderate quality evidence).Conclusions: In this meta-analysis of patients with sepsis, the use of vitamin C was not associated with reduction in 28-day mortality, but vitamin C may have a positive effect in improving organ function. As the certainty of evidence was low, Larger RCTs were needed.
Biochemical and Structural Characterization of Quizalofop-Resistant Wheat Acetyl-Coa Carboxylase
Authors: Bough Raven , Dayan Franck 
Published: September 2021

Abstract
A novel nucleotide mutation in ACC1 resulting in an alanine to valine amino acid substitution in acetyl-CoA carboxylase (ACCase) at position 2004 of the Alopecurus myosuroides reference sequence (A2004V) imparts quizalofop resistance in wheat. Genotypes endowed with one or two homozygous mutant ACC1 homoelogs are 7- and 68-fold more resistant to quizalofop than a wildtype variety in greenhouse experiments, respectively. In vitro assays of ACCase activities in protein extracts from these varieties reveal a 3.8- and 39.4-fold increase in resistance to quizalofop in the single and double-mutants relative to the wildtype. The A2004V mutation does not alter the specific activity of wheat ACCase, suggesting that ACCase mutants retain their normal catalytic functions. Modeling of wildtype and quizalofop-resistant wheat ACCase demonstrates that the A2004V amino acid substitution causes a reduction in the volume of the binding pocket that hinders quizalofop’s interaction with ACCase. Docking studies confirm that the mutation reduces the binding affinity of quizalofop. Interestingly, the models suggest that the A2004V mutation does not affect haloxyfop binding. Follow up in vivo and in vitro experiments reveal that the mutation, in fact, imparts negative cross-resistance to haloxyfop, with quizalofop-resistant varieties exhibiting more sensitivity to haloxyfop than the wildtype variety.
Research on Modeling of Government Debt Risk Comprehensive Evaluation Based on Multidimensional Data Mining
Authors: ChaoYing Li , Da Wu Xiang , Hui Zhao En 
Published: September 2021

Abstract
In order to solve the problems of low accuracy of data mining, high relative error rate of evaluation and long time of evaluation in traditional government debt risk evaluation methods, this paper proposes a modeling method of government debt risk comprehensive evaluation based on multidimensional data mining. The MAFIA algorithm is used for multidimensional mining of government debt risk data, and K-means clustering algorithm is used for clustering processing of mined data. According to the clustering results, the KMV model is constructed, and the uncertainty factor is used to modify the model, so as to realize the comprehensive evaluation of government debt risk by using the modified KMV model. The experimental results show that the accuracy rate of government debt risk data mining is always above 91%, the relative error rate of evaluation is always below 3.4%, and the average evaluation time is 0.71s, the practical application effect is good.
Simultaneous Radio and Optical Polarimetry of GRB 191221B Afterglow
Authors: Urata Yuji , Toma Kenji , Covino Stefano , Wiersema Klaas , Huang Kuiyun , Shimoda Jiro , Kuwata Asuka , Nagao Sota , Asada Keiichi , Nagai Hiroshi , Takahashi Satoko , Chung Chao-En , Petitpas Glen , Yamaoka Kazutaka , Tashiro Makoto 
Published: September 2021

Abstract
Gamma-ray bursts (GRBs) are the most luminous gamma-ray transients in the universe, and are utilized as probes of early stars, gravitational wave counterparts, and collision less shock physics. For understanding the fundamental physical quantities of GRB jets and their environments as well as their emission mechanism, coordinated multi-wavelength (semi-)simultaneous measurements are crucial as the global communities demonstrated in the past three decades. In spite of studies on polarimetry of GRBs in individual wavelengths that characterized intriguing properties of prompt emission and afterglow, no coordinated multi-wavelength measurements has yet been performed. Here, we report the first coordinated simultaneous polarimetry in the optical and radio bands for the afterglow associated to the typical long GRB 191221B. Our observations successfully caught the radio emission, which is not affected by synchrotron self-absorption, and show that the emission is depolarized in the radio band in comparison with the optical one. This result excludes a simple one-zone model that the polarization degree is nearly constant above the synchrotron self-absorption frequency, and has important implications for plasma-scale turbulent magnetic fields and existence of cool electrons. Our simultaneous polarization angle measurement supports the latter model rather than the former one. The existence of cool electrons increases the estimate of the total jet energy by a factor of > 2 for this typical GRB. Further coordinated multi-wavelength polarimetric campaigns would improve our understanding of the total jet energies and magnetic field configurations in the emission regions of various types of GRBs, which are required to comprehend the mass scales of their progenitor systems and the physics of collisionless shocks.
Micronodular basal cell carcinoma of the scrotum- a case report and review of the literature
Authors: Younes Mohammad , Kouba Lamia , Almsokar Hanaa , Badran Ayham 
Published: September 2021

Abstract
Introduction: Basal cell carcinoma (BCC) is the most common non-melanotic skin cancer. It has variable clinical and histological subtypes that vary in their aggressiveness and liability to recurrence and metastasis. Chronic ultraviolet radiation exposure is considered to be the main risk factor for developing BCC; therefore it typically arises on sun-exposed skin, mainly the head and neck. Case presentation: We present the case of a 55-year-old male who presented with a lesion on the scrotum for 2 years. The lesion was clinically presumed benign and initially treated with curettage. Microscopic examination revealed an incompletely resected micronodular BCC with sebaceous differentiation. Therefore, a second excisional biopsy was performed to completely excise the incidentally-discovered malignant tumor. Conclusion: We report the first case of micronodular BCC arising on the scrotum. The goal of our article is to draw clinicians’ attention to the possible involvement of unexposed skin with BCC and we highlight the importance of accurate diagnosis and prompt treatment due the aggressive nature of micronodular BCC.
Chronic active EB virus infection with hemophagocytic lymphohistiocytosis and progression to aggressive NK cell leukemia: a case report and review of the literature
Authors: Gao Li-min , Li Hui-fang , Zhao Sha , Zhang Wen-Yan , Li Qiang , Chen Zi-Hang , De souza Veylenta Audry , Biju Bincy Ann , Liu Weiping 
Published: September 2021

Abstract
BackgroundThe clinical trend of EBV infected patients has been always the focus of attention. It is extremely difficult to identify which patients may resolve spontaneously or proceed to chronic stage or proceed rapidly.Case descriptionWe report a rare case of chronic active EBV infection (CAEBV) with hemophagocytic lymphohistiocytosis progressed to childhood aggressive NK cell leukemia (ANKL) and cured by allogeneic hematopoietic stem cell transplantation.ConclusionsThe EBV infection of T/NK cells can lead to CAEBV, and if the T/NK cells carry HLH related gene mutation, it is easy to accompany with the hemophagocytic syndrome, and this type of CAEBV may rapidly progress to ANKL or other neoplastic diseases. Therefore, we should reinforce the knowledge on this kind of patients to receive appropriate and timely treatment.
Lymphocyte monocyte ratio is an effective and simple predictor for nosocomial influenza outbreaks
Authors: Junping Fan , Fanhang Ke , Fangyan Sun , Xinlun Tian , Meng Xiao , Luo Wang , Jinglan Wang , Wenzhao Chai 
Published: September 2021

Abstract
ObjectivesNosocomial influenza outbreak detection remains challenging. We evaluated the diagnostic utility of blood cell parameters, along with their capacity to differentiate between hospital acquired influenza and coronavirus disease 2019 (COVID-19).MethodsWe retrospectively analyzed patients diagnosed with nosocomial influenza from January 2017 to December 2019, and patients with COVID-19 in early 2020 at a tertiary teaching hospital in Beijing, China. We compared the differences between blood cell count and ratios (lymphocyte-to-monocyte ratio [LMR], neutrophil-to-lymphocyte ratio [NLR], lymphocyte-to-platelet ratio [LPR]) at symptom onset, before (admission), and after (recovery) nosocomial influenza. We also compared the abovementioned parameters between influenza and COVID-19 patients.ResultsLymphocyte count, LMR, and LPR were significantly lower in the symptom onset than in the admission and recovery groups (p < 0.001), while NLR was higher (p < 0.001). LMR and NLR exhibited similar and consistent tendencies among different subgroups of patients with nosocomial influenza (p < 0.001). The area under the receiver operating curve (AUC) of LMR, NLR, LPR, and lymphocyte count were 0.914, 0.872, 0.806, and 0.866, respectively. The optimal LMR cut-off value was 2.50, with specificity and sensitivity of 92.0% and 81.3%, respectively. Peripheral blood cell ratios can help diagnose nosocomial influenza significantly earlier than conventional methods. For differentiating influenza and COVID-19, the AUCs of LMR was 0.825.ConclusionsLMR effectively predicts nosocomial influenza outbreaks, particularly during the COVID-19 pandemic when simultaneous transmission can be a substantial threat.
Retrospective longitudinal analysis of the effects of postnatal weight gain on the timing and tempo of puberty and menarche in a cohort of Italian girls
Authors: Stagi Stefano , Ferrari Vittorio , Stefanucci Simona , Ferrari Marta , Ciofi Daniele 
Published: September 2021

Abstract
Objectiveover the last few decades there has been a progressive decline in the average age of onset of pubertal development stages in both sexes. The increase in the prevalence of childhood obesity seems to play an important role in this phenomenon.Designwe undertook a retrospective, longitudinal evaluation of the average age of thelarche and menarche to evaluate the relationship between BMI and weight change during the first years of life and the timing and tempo of puberty.Methodswe evaluated data for 577 Italian girls born between 1995 and 2003. We collected the main auxological and clinical parameters, including age at B2 and at menarche, BMI SDS at B2 and menarche, gestational age and birth weight and Z-score change from birth weight (BW) to BMI at B2 and menarche.Resultsthe mean age of B2 was 10.06 ± 1.03 years and the mean age of menarche was 12.08 ± 1.02 years. Age at B2 and menarche were inversely correlated with BMI SDS (p < 0.0001). Both age at menarche and at thelarche have an inverse relationship with the Z-score change from birth weight and BMI at menarche and thelarche respectively (p < 0.0001).Conclusionsour data confirm a significant relationship between BMI and age of B2 and menarche. We observed a clear relationship among weight change during the first years of life, age at thelarche and menarche and the duration of puberty, demonstrating the importance of weight and weight gain in determining the timing and tempo of pubertal changes and growth.
An Ensemble-Based Approach for Estimating Personalized Intraocular Lens Power
Authors: Moutari Salissou , Moore Jonathan E 
Published: September 2021

Abstract
This study introduced a new approach for the calculation of personalized intraocular lens power, which used an ensemble of regression models to devise a more accurate and robust prediction of the effective lens position. The concept of cross-validation is used to rigorously assess the performance of the devised formula against the most commonly used published formulae. The results from this study show that overall, the proposed approach outperforms the most commonly used modern formulae (namely, SRK/T, Hoffer Q, Holladay I, and Haigis) in terms of mean absolute prediction errors and prediction accuracy i.e., the percentage of eyes within ± 0.5D and ± 1 D ranges of prediction, for various ranges of axial lengths of the eyes. The results are obtained using three models of lens (two monofocal and one multifocal). Furthermore, the proposed formula exhibited some promising features in terms of robustness. This particular characteristic enables the new formula to cope with the variations in the axial length, the pre-operative anterior chamber depth as well as the keratometric readings of the corneal power; hence mitigating the impact of measurement accuracy for these parameters.
Computational Modeling of Chromatin Accessibility Identified Important Epigenomic Regulators
Authors: Zhao Yanding , Dong Yadong , Hong Wei , Jiang Chongming , Yao Kevin , Cheng Chao 
Published: September 2021

Abstract
Chromatin accessibility is essential for transcriptional activation of genomic regions. It is well established that transcription factors (TFs) and histone modifications (HMs) play critical roles in chromatin accessibility regulation. However, there is a lack of studies that quantify these relationships. Here we constructed a two-layer model to predict chromatin accessibility by integrating DNA sequence, TF binding, and HM signals. By applying the model to two human cell lines (GM12878 and HepG2), we found that DNA sequences had limited power for accessibility prediction, while both TF binding and HM signals predicted chromatin accessibility with high accuracy. According to the HM model, HM features determined chromatin accessibility in a cell line shared manner, with the prediction power attributing to five core HM types. Results from the TF model indicated that chromatin accessibility was determined by a subset of informative TFs including both cell line-specific and generic TFs. The combined model of both TF and HM signals did not further improve the prediction accuracy, indicating that they provide redundant information in terms of chromatin accessibility prediction. The TFs and HM models can also distinguish the chromatin accessibility of proximal versus distal transcription start sites with high accuracy.
Morphometric Analysis Program and Quantitative Positron Emission Tomography in Presurgical Localization in MRI-negative Epilepsies: a Simultaneous PET/MRI Study
Authors: Guo Kun , Wang Jingjuan , Wang Zhenming , Wang Yihe , Cui Bixiao , Zhao Guoguang , Lu Jie 
Published: September 2021

Abstract
Purpose: To evaluate morphometric analysis program (MAP) and quantitative positron emission tomography (QPET) in epileptogenic zone (EZ) identification using a simultaneous positron emission tomography/magnetic resonance imaging (PET/MRI) system in MRI-negative epilepsies.Methods: Seventy-one localization-related MRI-negative epilepsies underwent preoperative simultaneous PET/MRI examination and surgical resection were enrolled retrospectively. MAP was performed on T1-weighted volumetric sequence and QPET was analyzed using statistical parametric mapping (SPM) with comparison to age-, and gender- matched normal controls. The sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) of MAP, QPET, MAP+ QPET and MAP/QPET in EZ localization were assessed. The correlations between surgical outcome and modalities concordant with cortical resection were analyzed.Results: Forty-five (63.4%) patients had Engel I seizure outcome. The sensitivity, specificity, PPV and NPV of MAP was 64.4%, 69.2%, 78.3% and 52.9%, respectively. The sensitivity, specificity, PPV, NPV of QPET was 73.3%, 65.4%, 78.6%, and 58.6%, respectively. MAP+QPET, defined as two tests concordant with cortical resection, had reduced sensitivity (53.3%) but increased specificity (88.5%) relative to individual tests. MAP/QPET, defined as one or both tests concordant with cortical resection, had increased sensitivity (86.7%) but reduced specificity (46.2%) relative to individual tests. The regions determined by MAP, QPET, MAP+QPET or MAP/QPET concordant with cortical resection was significantly associated with seizure-free outcome, MAP+QPET performed best. Conclusion: The combination of MAP and QPET imaging play a complementary role in EZ localization and this approach can be readily improved surgical outcome in MRI-negative epilepsies.