bluuebunny/crossref_metadata_2025_split huggingface dataset:
Beyond multi view deconvolution for inherently aligned fluorescence tomography
Authors: Ancora Daniele , Valentini Gianluca , Pifferi Antonio , Bassi Andrea 
Published: August 2021

AbstractIn multi-view fluorescence microscopy, each angular acquisition needs to be aligned with care to obtain an optimal volumetric reconstruction. Here, instead, we propose a neat protocol based on auto-correlation inversion, that leads directly to the formation of inherently aligned tomographies. Our method generates sharp reconstructions, with the same accuracy reachable after sub-pixel alignment but with improved point-spread-function. The procedure can be performed simultaneously with deconvolution further increasing the reconstruction resolution.
In-situ study of electrochemical migration of tin in the presence of bromide ion
Authors: Lee Ee Lynn , Haseeb A. S. M. A. , Basirun Wan Jeffrey , Wong Yew Hoong , Sabri Mohd Faizul Mohd , Low Boon Yew 
Published: August 2021

AbstractThe miniaturization of electronic devices and the consequent decrease in the distance between conductive lines have increased the risk of short circuit failure due to electrochemical migration (ECM). The presence of ionic contaminants affects the ECM process. This work systematically investigates the ECM of tin (Sn) in the presence of bromide ions (Br−) in the range of 10−6 M to 1.0 M. Water drop test (WDT) was conducted in the two-probe semiconductor characterization system under an optical microscope as an in-situ observation. Polarization test was carried out to study the correlation between the corrosion properties of Sn and its ECM behaviour. The products of ECM were characterized by scanning electron microscope coupled with an energy dispersive X-rays spectrometer (SEM/EDX) and X-ray photoelectron spectrometer (XPS). The results confirm that the rate of anodic dissolution of Sn monotonously increases with the Br− concentration. However, the probability of ECM failure follows a normal distribution initially, but later increases with the Br− concentration. The main products of the ECM reactions are identified as Sn dendrites and tin hydroxide precipitates. The mechanisms of the ECM process of Sn in the presence of Br− are also suggested.
Interactions of boron released from surface pre-reacted glass ionomer with enamel/dentin and its effect on pH
Authors: Hiraishi Noriko , Sayed Mahmoud , Hill Robert , Tagami Junji , Hayashi Fumiaki 
Published: August 2021

AbstractThis study investigated the interaction of borate ions released from surface pre-reacted glass ionomer (S-PRG) fillers with the biological apatites of enamel and dentin using solid-state (SS) magic-angle spinning nuclear magnetic resonance (MAS-NMR) spectroscopy analysis. We further evaluated the effect of borate ions on the pH change. Bovine enamel and dentin powder were submerged in S-PRG eluate (with borate ion concentration of 100 mM) for 3 h, then washed with deionized water (DW). The dried enamel and dentin specimens were used for 11B SS-NMR and Fourier transform infrared spectroscopy (FT-IR) analysis. Enamel and dentin blocks were treated with three solutions: DW (control), S-PRG eluent and borate solution (100 mM). The treated samples were submerged in the demineralization solution and the pH was measured using a pH meter daily for 6 days. The surfaces of the enamel and dentin blocks were then observed using field emission scanning electron microscopy (FE-SEM). SS-NMR analysis revealed the presence of adsorbed borate ions in the enamel and dentin samples in a tetra-coordinated form. The pH results demonstrated an increase in pH values in the S-PRG and borate groups. SEM images showed that the surfaces of the control group were demineralized, whereas the surfaces of the S-PRG and borate groups were intact. These results concluded that borate ions could be adsorbed to enamel and dentin in the tetra-coordinated form. Borate ions possess a buffer capacity which may help to protect the tooth structure against acid attacks and promote remineralization.
Interaction between the level of immunoglobulins and number of somatic cells as a factor shaping the immunomodulating properties of colostrum
Authors: Puppel Kamila , Gołębiewski Marcin , Slósarz Jan , Grodkowski Grzegorz , Solarczyk Paweł , Kostusiak Piotr , Grodkowska Kinga , Balcerak Marek , Sakowski Tomasz 
Published: August 2021

AbstractThe aim of this study was to investigate the association between immunoglobulins and SCC as a factor in shaping the content of the immunostimulatory components of colostrum. Seventy-eight multiparous Polish Holstein–Friesian cows were selected for the experiment. Colostrum samples were collected immediately after calving (up to a max. of 2 h). The cows were divided into groups according to the following levels: Immunoglobulins (IG class)—(IG1) over 50 g/L, (IG2) up to 50 g/L; SCC class—(SCC1) up to 400 000/ml, (SCC2) 400–800 000/ml, (SCC3) over 800 000/ml. Colostrum assigned to the IG1 SCC1 group had a statistically significant higher (p ≤ 0.01) concentration of both whey proteins and fatty acids compared to the IG1 SCC2 and SCC3 groups. The concentration of IgG, IgM, and IgA was shown to be higher in IG1 SCC1 than IG2 SCC3 by 226%, 149%, and 115%, respectively. The concentration of lactoferrin was shown to be higher in IG1 SCC1 than IG2 SCC3 by 149%. The determination of colostrum quality based on the concentration of immunoglobulins in the colostrum may not be sufficient because serum IgG concentrations at birth show a linear increase relative to colostrum SCC. A breakdown of colostrum into quality classes, taking into account the level of SCC, should therefore be introduced.
Evidence of an epidemic spread of KPC-producing Enterobacterales in Czech hospitals
Authors: Kraftova Lucie , Finianos Marc , Studentova Vendula , Chudejova Katerina , Jakubu Vladislav , Zemlickova Helena , Papagiannitsis Costas C. , Bitar Ibrahim , Hrabak Jaroslav 
Published: August 2021

AbstractThe aim of the present study is to describe the ongoing spread of the KPC-producing strains, which is evolving to an epidemic in Czech hospitals. During the period of 2018–2019, a total of 108 KPC-producing Enterobacterales were recovered from 20 hospitals. Analysis of long-read sequencing data revealed the presence of several types of blaKPC-carrying plasmids; 19 out of 25 blaKPC-carrying plasmids could be assigned to R (n = 12), N (n = 5), C (n = 1) and P6 (n = 1) incompatibility (Inc) groups. Five of the remaining blaKPC-carrying plasmids were multireplicon, while one plasmid couldn’t be typed. Additionally, phylogenetic analysis confirmed the spread of blaKPC-carrying plasmids among different clones of diverse Enterobacterales species. Our findings demonstrated that the increased prevalence of KPC-producing isolates was due to plasmids spreading among different species. In some districts, the local dissemination of IncR and IncN plasmids was observed. Additionally, the ongoing evolution of blaKPC-carrying plasmids, through genetic rearrangements, favours the preservation and further dissemination of these mobile genetic elements. Therefore, the situation should be monitored, and immediate infection control should be implemented in hospitals reporting KPC-producing strains.
Regional metabolic and network changes in Meige syndrome
Authors: Liu Jiayu , Li Lei , Li Yuan , Wang Qian , Liu Ruen , Ding Hu 
Published: August 2021

AbstractTo contribute to the understanding of the aetiology and pathogenesis of Meige syndrome, the metabolic networks of patients with Meige syndrome were investigated using 18F-fluoro-D-glucose positron emission tomography (18F-FDG-PET) imaging of cerebral glucose metabolism. Fifty right-handed and unmedicated primary Meige syndrome patients enrolled between September 2017 and September 2020 at the Department of Neurosurgery, Peking University People’s Hospital, and 50 age- and sex-matched healthy control subjects participated in the study. Metabolic connectivity and graph theory analysis were used to investigate metabolic network differences based on 18F-FDG-PET images. Glucose hypometabolism was detected in the left internal globus pallidus and parietal lobe, right frontal lobe and postcentral gyrus, and bilateral thalamus and cerebellum of patients with Meige syndrome. Clustering coefficients (Cps) (density threshold: 16–28%; P < 0.05) and shortest path lengths (Lps) (density threshold: 10–15%; P < 0.05) were higher in Meige syndrome patients than in healthy controls. Small-worldness was lower in Meige syndrome patients than in healthy controls, and centrality was significantly lower in the right superior occipital gyrus and pallidum and higher in the right thalamus. Hypometabolism in the globus pallidus and thalamus may indicate basal ganglia-thalamocortical motor circuit abnormalities as a pathogenic mechanism of Meige syndrome, providing a possible explanation for the efficacy of deep brain stimulation (DBS) in improving symptoms. Meige syndrome patients had abnormal small-world properties. Centrality changes in the right pallidus and thalamus verified the important roles of these regions in the pathogenesis of Meige syndrome.
Potential therapeutic effect of thymoquinone and/or bee pollen on fluvastatin-induced hepatitis in rats
Authors: Mohamed Amro E. , El-Magd Mohammed A. , El-Said Karim S. , El-Sharnouby Mohamed , Tousson Ehab M. , Salama Afrah F. 
Published: August 2021

AbstractHepatitis is one of earlier, but serious, signs of liver damage. High doses of statins for a long time can induce hepatitis. This study aimed to evaluate and compare the therapeutic potential of thymoquinone (TQ) and bee pollen (BP) on fluvastatin (F)-induced hepatitis in rats. Rats were randomly divided into: group 1 (G1, control), G2 (F, hepatitis), G3 (F + TQ), G4 (F + BP), and G5 (F + TQ + BP). Single treatment with TQ or BP relieved fluvastatin-induced hepatitis, with best effect for the combined therapy. TQ and/or BP treatment significantly (1) reduced serum levels of alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, gamma glutamyl transpeptidase, and total bilirubin, (2) decreased malondialdehyde levels and increased level of reduced glutathione, and activities of glutathione peroxidase and catalase in the liver, (3) improved liver histology with mild deposition of type I collagen, (4) increased mRNA levels of transforming growth factor beta 1, nuclear factor Kappa B, and cyclooxygenase 1 and 2, and (5) decreased tumor necrosis factor alpha and upregulated interleukin 10 protein in the liver. These data clearly highlight the ability of TQ and BP combined therapy to cause better ameliorative effects on fluvastatin-induced hepatitis than individual treatment by each alone.
Acute ischemic stroke triggers a cellular senescence-associated secretory phenotype
Authors: Torres-Querol Coral , Torres Pascual , Vidal Noemí , Portero-Otín Manel , Arque Gloria , Purroy Francisco 
Published: August 2021

AbstractSenescent cells are capable of expressing a myriad of inflammatory cytokines and this pro-inflammatory phenomenon is known as senescence-associated secretory phenotype (SASP). The contribution of this phenomenon in brain ischemia was scarce. A mouse model of transient focal cerebral ischemia by compressing the distal middle cerebral artery (tMCAo) for 60 min was used. SASP, pro-inflammatory cytokines and cell cycle mRNAs levels were quantified at 30-min and 72 h post-surgery. Immunohistochemistry in paraffin embedded human brain slides and mouse brain tissue was performed. Our results showed an increase of both p16 and p21 mRNA restricted to the infarct area in the tMCAo brain. Moreover, there was an induction of Il6, Tnfa, Cxc11, and its receptor Cxcr2 mRNA pro-inflammatory cytokines with a high positive correlation with p16/p21 mRNA levels. The p16 was mainly shown in cytoplasm of neurons and cytoplasm/membrane of microglial cells. The p21 was observed in membrane of neurons and also it showed a mixed cytoplasmic and membranous pattern in the microglial cells. In a human stroke patient, an increase of P16 in the perimeter of the MCA infarct area was observed. These suggest a role of SASP in tMCAo mouse model and in human brain tissue. SASP potentially has a physiological role in acute ischemic stroke and neurological function loss.
Exo-selective intermolecular Diels–Alder reaction by PyrI4 and AbnU on non-natural substrates
Authors: Kashyap Rajnandani , Yerra Naga Veera , Oja Joachyutharayalu , Bala Sandeepchowdary , Potuganti Gal Reddy , Thota Jagadeshwar Reddy , Alla Manjula , Pal Debnath , Addlagatta Anthony 
Published: August 2021

AbstractThe 100-year-old Diels–Alder reaction (DAr) is an atom economic and elegant organic chemistry transformation combining a 1,3-diene and a dienophile in a [4+2] cycloaddition leading to a set of products with several stereo centres and multiple stereoisomers. Stereoselective [4+2] cycloaddition is a challenge. Here, we describe two natural enzymes, PyrI4 and AbnU performing stereospecific intermolecular DAr on non-natural substrates. AbnU catalyses a single exo-stereoisomer by 32-fold higher than the background. PyrI4 catalyses the same stereoisomer (15-fold higher) as a major component (>50%). Structural, biochemical and fluorescence studies indicate that the dienophile enters first into the β-barrel of the enzymes followed by the 1,3-diene, yielding a stereospecific product. However, if some critical interactions are disrupted to increase the catalytic efficiency, stereoselectivity is compromised. Since it is established that natural enzymes can carry out intermolecular DAr on non-natural substrates, several hundreds of Diels-Alderases available in nature could be explored.
Estrogen receptor actions in colitis
Authors: Garcia-Villatoro Erika L. , Allred Clinton D. 
Published: August 2021

Abstract
In recent years, researchers have demonstrated that estrogen and its receptors, aside from their role in regulating several biological functions, contribute to the development and progression/severity of inflammatory bowel diseases (IBDs). IBDs include both ulcerative colitis (UC) and Crohn’s disease (CD). Epidemiological data indicate a clear difference in the incidence, severity, and complications of IBDs between sexes. Men present a higher risk of developing colitis than women and a higher risk of developing colorectal cancer, a common complication of this condition. However, fluctuations of estrogen levels have yielded inconsistent data, where oral contraceptives and hormone replacement therapy have been associated with an increased risk of IBDs in premenopausal women but significantly reduce disease activity after menopause. Likewise, improvement of symptoms related to CD has been reported during pregnancy, but not in UC, who often experience worsening symptoms. In the colonic epithelium, estrogen receptor β (ERβ) is the predominant form of the protein expressed, and it helps maintain normal epithelial function and organization. Preclinical data suggest that ER expression and activation via estrogen confers different responses on disease severity depending on the model used to induce colitis, which may reflect what is observed in patients with IBDs. Hence, this review aims to provide an overview of estrogen and its receptors, particularly ERβ, in the pathophysiology of IBDs.
Improved composite adaptive fault‐tolerant control for dynamic positioning vehicle subject to the dead‐zone nonlinearity
Authors: Zhang Guoqing , Yao Mingqi , Zhang Wenjun , Zhang Weidong 
Published: August 2021

AbstractIn order to tackle the marine practical constraints, for example the actuator faults, the dead‐zone input, an improved composite adaptive neural control algorithm is proposed for dynamic positioning vehicles in presence of the unknown external disturbances. In the algorithm, the robust neural damping technique is employed to remodel the system model uncertainty and suppress the external interference. As for the dead‐zone input, the dead‐zone inverse model is constructed to derive the corresponding compensating terms. That could effectively release the constraints from the actuator faults and the dead‐zone non‐linearity. Furthermore, for merits of the composite intelligent learning method, one designs the serial‐parallel estimation model to estimate the related velocity variables. The corresponding prediction error could be applied in the design of adaptive law. That could effectively improve the accuracy of parameter estimation and facilitate the robustness of the closed‐loop system. The semi‐global uniformly ultimately bounded stability is guaranteed for all error signals in the closed‐loop system by utilizing the Lyapunov theory. Finally, the validity of the proposed algorithm is demonstrated through the simulation experiments.
Multi‐objective electrical demand response policy generation considering customer response behaviour learning: An enhanced inverse reinforcement learning approach
Authors: Lin Junhao , Zhang Yan , Xu Shuangdie , Yu Haidong 
Published: August 2021

AbstractDemand response (DR) is an effective load management method. To attract customers to participate, DR policies need to both satisfy customers' individual DR habits and be economically profitable. However, customers’ individual DR habits are hard to be formulated with few hypotheses when other objectives are simultaneously considered. To tackle this challenge, a novel DR behavioural learning method is proposed. We learn customers’ DR habits by an inverse reinforcement learning (IRL) method to reduce the subjectivity in DR model formulation. Meanwhile, in contrast to traditional learning‐based methods, the proposed method can adapt to multiple DR objectives more than just following customers’ DR habits, like obtaining higher economic revenues. Additionally, we consider the diversity and changes of customer DR behaviour patterns and offer an enhancement for the proposed DR behavioural learning method via building a DR pattern clustering and inference module. The proposed method can work with customer‐side energy storage systems to diversify the DR policies and make the DR behaviours more flexible. Case studies show the proposed method can reduce about 10–20% behavioural learning deviations than the compared model‐based methods, while daily charges of the proposed method can be further reduced by over 4% than the compared supervised‐learning‐based methods.
Balmer continuum enhancement detected in a mini flare observed with IRIS
Authors: Joshi Reetika , Schmieder Brigitte , Heinzel Petr , Tomin James , Chandra Ramesh , Vilmer Nicole 
Published: August 2021

Context. Optical and near-UV continuum emissions in flares contribute substantially to the flare energy budget. Two mechanisms play an important role for continuum emission in flares: hydrogen recombination after sudden ionization at chromospheric layers, and transportation of the energy radiatively from the chromosphere to lower layers in the atmosphere, the so-called back-warming.
Aims. The aim of the paper is to distinguish between these two mechanisms for the excess of the Balmer continuum observed in a flare.
Methods. We combined the observations of the Balmer continuum obtained with the Interface Region Imaging Spectrograph (IRIS) (spectra and slit-jaw images (SJIs) 2832 Å) and hard X-ray (HXR) emission detected by the Fermi/Gamma Burst Monitor (GBM) during a mini flare. The calibrated Balmer continuum was compared to non-local thermodynamic equilibrium (LTE) radiative transfer flare models, and the radiated energy was estimated. Assuming thick target HXR emission, we calculated the energy of the nonthermal electrons detected by the Fermi/GBM and compared it to the radiated energy.
Results. The favorable argument of a relation between the Balmer continuum excess and the HXR emission is that there is a good time coincidence between them. In addition, the shape of the maximum brightness in the 2832 SJIs, which is mainly due to this Balmer continuum excess, is similar to that of the Fermi/GBM light curve. The electron-beam flux estimated from Fermi/GBM between 109 and 1010 erg s−1 cm−2 is consistent with the beam flux required in non-LTE radiative transfer models to obtain the excess of Balmer continuum emission observed in this IRIS spectra.
Conclusions. The low-energy input by nonthermal electrons above 20 keV is sufficient to produce the enhancement in the Balmer continuum emission. This could be explained by the topology of the reconnection site. The reconnection starts in a tiny bald-patch region, which is transformed dynamically into an X-point current sheet. The size of the interacting region would be below the spatial resolution of the instrument.
PSR J2222−0137
Authors: Guo Y. J. , Freire P. C. C. , Guillemot L. , Kramer M. , Zhu W. W. , Wex N. , McKee J. W. , Deller A. , Ding H. , Kaplan D. L. , Stappers B. , Cognard I. , Miao X. , Haase L. , Keith M. , Ransom S. M. , Theureau G. 
Published: August 2021

Context. The PSR J2222−0137 binary system has a set of features that make it a unique laboratory for tests of gravity theories.
Aims. To fully exploit the system’s potential for these tests, we aim to improve the measurements of its physical parameters, spin and orbital orientation, and post-Keplerian parameters, which quantify the observed relativistic effects.
Methods. We describe an improved analysis of archival very long baseline interferometry (VLBI) data, which uses a coordinate convention in full agreement with that used in timing. We have also obtained much improved polarimetry of the pulsar with the Five hundred meter Aperture Spherical Telescope (FAST). We provide an improved analysis of significantly extended timing datasets taken with the Effelsberg, Nançay, and Lovell radio telescopes; this also includes previous timing data from the Green Bank Telescope.
Results. From the VLBI analysis, we have obtained a new estimate of the position angle of the ascending node, Ω = 189−18+19 deg (all uncertainties are 68% confidence limits), and a new reference position for the pulsar with an improved and more conservative uncertainty estimate. The FAST polarimetric results, and in particular the detection of an interpulse, yield much improved estimates for the spin geometry of the pulsar, in particular an inclination of the spin axis of the pulsar of ∼84 deg. From the timing, we obtain a new ∼1% test of general relativity (GR) from the agreement of the Shapiro delay parameters and the rate of advance of periastron. Assuming GR in a self-consistent analysis of all effects, we obtain much improved masses: 1.831(10) M⊙ for the pulsar and 1.319(4) M⊙ for the white dwarf companion; the total mass, 3.150(14) M⊙, confirms this as the most massive double degenerate binary known in the Galaxy. This analysis also yields the orbital orientation; in particular, the orbital inclination is 85.27(4) deg – indicating a close alignment between the spin of the pulsar and the orbital angular momentum – and Ω = 187.7(5.7) deg, which matches our new VLBI estimate. Finally, the timing also yields a precise measurement of the variation in the orbital period, Ṗb = 0.251(8) × 10−12 ss−1; this is consistent with the expected variation in the Doppler factor plus the orbital decay caused by the emission of gravitational waves predicted by GR. This agreement introduces stringent constraints on the emission of dipolar gravitational waves.