bluuebunny/crossref_metadata_2025_split huggingface dataset:
Elevated Expression of the RNA-Binding Protein IGF2BP1 Enhances the Mrna Stability and Translation Efficiency of INHBA to Promote the Invasion and Migration of Esophageal Squamous Cancer Cells
Authors: Wang Juan-Juan , Chen Ding-Xiong , Zhang Yu , Cai Yan , Xu Xin , Hao Jia-Jie , Wang Ming-Rong 
Published: September 2021

Abstract
Background: The mechanisms underlying the occurrence and development of esophageal squamous cell carcinoma (ESCC) remains to be elucidated. The present study aims to investigate the roles and implications of IGF2BP1 overexpression in ESCC.Methods: IGF2BP1 protein expression in ESCC samples was assessed by immunohistochemistry (IHC), and the mRNA abundance of IGF2BP1 and INHBA was analyzed with TCGA datasets and by RNA in situ hybridization (RISH). Cell viability, migration, invasion and in vivo metastasis assays were performed to explore the roles of IGF2BP1 overexpression in ESCC. RNA immunoprecipitation sequencing (RIP-seq) and mass spectrometry were applied to identify the target RNAs and interacting proteins of IGF2BP1, respectively. RIP-PCR, RNA pulldown, immunofluorescence (IF), gene-specific m6A PCR and RNA stability assays were used to uncover the molecular mechanisms underlying the malignant phenotypes of ESCC cells caused by IGF2BP1 dysregulation. The methylation level of the IGF2BP1 promoter region was detected by methylation-specific PCR (MSP-PCR). BTYNB, a small molecular inhibitor was evaluated for its inhibitory effect on the malignant phenotypes of ESCC cells.Results: IGF2BP1 overexpression was detected in ESCC tissues and associated with the depth of tumor invasion. Knockdown of IGF2BP1 inhibited ESCC cell invasion and migration as well as tumor metastasis. Mechanistically, we observed that IGF2BP1 bound and stabilized INHBA mRNA and then enhanced the translation of INHBA, leading to the activation of Smad2/3 signaling, thus promoting malignant phenotypes. The mRNA level of INHBA was upregulated in ESCC tissues as well. Furthermore, IGF2BP1 interacted with G3BP stress granule assembly factor 1 (G3BP1) and activated the INHBA-Smad signaling. BTYNB abolished this activated signaling and significantly attenuated the malignant phenotypes of ESCC cells. In addition, IGF2BP1 mRNA expression in ESCC cells was negatively correlated with the level of its promoter methylation.Conclusions: Elevated expression of IGF2BP1 is a frequent event in ESCC tissues and might be a candidate biomarker for the disease. IGF2BP1 overexpression promotes the invasion and migration of ESCC cells by activating the INHBA-TGF-β-Smad2/3 pathway, providing a potential therapeutic target for ESCC patients with high expression of IGF2BP1.
A Framework To Estimate Loss And Damage (L&amp;D) To Floods In a Changing Climate, Using Capital-Based Approach
Authors: Bose Devdyuti , Mishra Trupti 
Published: September 2021

Abstract
Although there exist many mitigation and adaptation responses for disaster management, with specific reference to floods, still there are some residual impacts which arise from insufficient mitigation and inadequate adaptation, known as loss and damage (L&D). Literature has identified familiar methodologies, namely Damage and Loss Assessment, Post Disaster Needs Assessment, Catastrophe Simulation, Hazus-MH, and econometric models to quantify “loss” and “damage” separately, but, given that the starting point of L&D, being the livelihood vulnerability context of the individuals, there is a need for comprehensive approach to quantify residual “loss and damage” on the livelihood assets. This study has adopted a bottom-up approach, Sustainable Livelihood Framework and revised to provide the quantitative estimates of loss and damage of floods to the five types of capital assets (human, social, physical, financial, natural), at the individual level. A panel regression model can be used to determine the losses inflicted to each capital asset, which can be further used to derive first and second order loss and damage estimates for identifying room(s) for flood recovery interventions. A set of individual specific and community specific characteristics play an important role in determining the influence of floods on each indicator of each type of capital asset. However, this model can find differential impacts of different types of disaster in different regions across different individuals, depending on their exposure.
Intestinal and Mucosal Microbiomic Response to Oral Challenge of Enterotoxigenic Escherichia Coli in Weaned Pigs
Authors: Peng Shan-Shan , Li Yingjie , Chen Qiuhong , Hu Qi , He Ying , Che Lianqiang , Jiang Ping-Ping 
Published: September 2021

Abstract
Enterotoxigenic Escherichia coli (ETEC) is closely associated with diarrhoea in children in resource-limited countries and of travellers’ diarrhoea. This study aims to investigate the change of ileal mucosal microbiome and ileal protein expression as well as their correlation in pigs by E. coli K88 (ETEC). Seven weaned male pigs were orally given 1 ×109 CFU of ETEC (ETEC, n = 7), and the other seven received saline (CON, n = 7). Ileal tissues were obtained 48 h after the ETEC challenge for both proteomic and mucosal microbiomic analyses. Nine proteins were altered in expression level in the ETEC group, including decreased expression of FABP1 and FABP6 involved in bile acid circulation. TLR-9 mediated pathway was also affected at transcription level with increased expression of SIGIRR and MyD88. Correlation analysis revealed correlations between the ileal proteins and mucosal bacterial taxa, including the positive correlation between Lactobacilli and PPP3CA (r = 0.9, p < 0.001), and negative correlation between Prevotella with CTNND1 (r = -0.7, p < 0.01). In conclusion, ETEC infection caused inflammation and impaired the circulation of bile acids, and the mucosal microbiome may affect the expression of intestinal proteins. Further studies are needed for exact roles of these affected processes in the pathogenesis of ETEC-triggered diarrhoea.
Impact of Estrogen Receptor Expression on Prognosis of Ovarian Cancer According to Antibody Clone Used for Immunohistochemistry: A Meta-analysis
Authors: Ng Chun Wai , Wong Kwong-kwok 
Published: September 2021

Abstract
BackgroundThe prognostic value of the expression of estrogen receptor (ER) subtypes ER⍺ and ERβ in ovarian cancer has previously been evaluated by meta-analyses. However, the results are contradictory and controversial. MethodsWe conducted an updated meta-analysis with stringent inclusion criteria to ensure homogeneous studies to determine the effect of ER subtypes on ovarian cancer prognosis. Articles were retrieved by systematic search of PubMed and Web of Science for articles dated up to June 2021. Only studies with known hazard ratio (HR) and antibody clone for immunochemistry (IHC) were included. Pooled HRs with the corresponding 95% confidence intervals (CIs) were calculated for the effect of ER⍺ and ERβ expression on ovarian cancer patient progression-free survival (PFS) and overall survival (OS).ResultsA total of 17 studies were included, of which 11 and 13 studies examined the relationships between ER⍺ expression and PFS and OS, respectively, and 5 and 7 studies examined the relationships between ERβ expression and PFS and OS, respectively. Neither ER⍺ expression (random-effects model; HR=0.99, 95% CI=0.83-1.18) nor ERβ expression (fixed-effects model; HR=0.94, 95% CI=0.69-1.27) was associated with PFS. Random-effects models showed that ER⍺ expression (HR=0.81, 95% CI=0.64-1.02) and ERβ expression (HR=0.75, 95% CI=0.50-1.13) were only marginally and not significantly associated with better OS. Subgroup analysis revealed that ER⍺ expression determined using antibody clone 1D5 (HR=0.75, 95% CI=0.64-0.88) and ERβ expression determined using ERβ1-specific-antibody clone PPG5/10 or EMR02 (HR=0.65, 95% CI=0.50-0.86) were associated with significantly better OS, but ER expression determined using other antibodies was not.ConclusionsBoth ER⍺ expression and ERβ expression determined using certain antibody clones are significantly associated with OS of ovarian cancer patients, which suggests that both ER subtypes are prognostic biomarkers for ovarian cancer. The findings of this study provide new insight into the impact of ER expression on ovarian cancer prognosis.
A novel Circular RNA circRBMS3 promote malignant tumor growth and metastasis through miR-424-eIF4B/YRDC axis
Authors: Gong Zhe , Shen Panyang , Wang Haitao , Zhu Jinjin , Wang Shengyu , Liang Kaiyu , xu Yining , Fan Shunwu , Shen Shuying , Fang Xiangqian , Liu Gang 
Published: September 2021

Abstract
Background

Circular RNAs (circRNAs) have been shown to have critical regulatory roles in tumorigenesis. However, the contribution of circRNAs to OS (osteosarcoma) remains largely unknown.
Methods

CircRNA deep sequencing was performed to the expression of circRNAs between OS and chondroma tissues. The regulatory and functional role of circRBMS3 (a circRNA derived from exons 7 to 10 of the RBMS3 gene, hsa_circ_0064644) upregulation was examined in OS and was validated in vitro and in vivo, upstream regulator and downstream target of circRBMS3 were both explored. RNA pull down, a luciferase reporter assay, biotin-coupled microRNA capture and fluorescence in situ hybridization were used to evaluate the interaction between circRBMS3 and micro (mi)-R-424-5p. For in vivo tumorgenesis experiments, Subcutaneous and Orthotopic xenograft OS mouse models were built.
Results

Expression of circRBMS3 was higher in OS tissues due to the regulation of adenosine deaminase 1-acting on RNA (ADAR1), an abundant RNA editing enzyme. Our in vitro data indicated that ShcircRBMS3 inhibits the proliferation and migration of malignant tumor cells. Mechanistically, we show that circRBMS3 can regulate eIF4B and YRDC, through ‘sponging’ miR-424-5p. Furthermore, knockdown of circRBMS3 inhibited malignant phenotypes and bone destruction of OS in vivo.
Conclusions

Our results reveal an important role for a novel circRBMS3 in the growth and metastasis of malignant tumor cells and provide a fresh perspective on circRNAs in OS progression.
Identification and Pyramid of QTLs Based on Rice Short-wide Grain CSSL-Z414, SSSL, DSSL and Candidate Gene Analysis of qGL11 and qGW5
Authors: Li Juan , Yang Hongxia , Xu Guangyi , Deng Keli , Yu Jinjin , Xiang Siqian , Zhou Kai , Zhang Qiuli , Li Ruxiang , Li Miaomiao , Ling Yinghua , Yang Zhenglin , He Guanghua , Zhao Fangming 
Published: September 2021

Abstract
BackgroundMost of rice agronomic traits as grain length etc. are complex traits controlled by multiple genes. Chromosome segment substitution lines (CSSLs) are ideal materials for dissecting and studying of these complex traits. ResultsA rice short-wide grain CSSL Z414 was identified among progeny of the recipient parent Xihui 18 (an indica restorer line) and the donor parent Huhan 3 (a japonica cultivar). Z414 carried 4 substitution segments (average length was 3.04 Mb), and displayed shorter panicle length and less number of primary branches, shorter, wider and larger grain, higher brown rice rate and chalkiness degree when compared with Xihui 18. Then, 9 quantitative trait loci (QTLs) for associated traits were identified using the secondary F2 population from Xihui 18 / Z414. Among them, 6 QTLs (qPL3, qGW5, qGL11, qRLW5, qRLW11, qGWT5) could be verified by corresponding single segment substitution lines (SSSLs, S1-S6). In addition, 4 QTLs (qGL3, qGL5, qCD3 and qCD5) were detected by S1 and S5, which was not detected by the F2 population. Thus, the grain length of Z414 was controlled by qGL11, qGL3 and qGL5, and the grain width of Z414 was answered by qGW5. Then by substitution mapping, qGL11 and qGW5 were delimited within the estimated substitution length of 1.42 and 1.14 Mb on chromosomes 11 and 5, and 4 and 2 candidate genes were found respectively for qGL11 and qGW5 by sequencing. However, only two had expression differences by qRT-PCR analysis. Finally, Analysis of QTL epistatic effects revealed that pyramid of qGL3 (a= 0.22) and qGL11 (a=-0.19) caused grain length of double segment substitution line (DSSL, D2) shorter than that of S5 (qGL11).ConclusionsWe developed a rice short –wide grain CSSL with 4 substitution segments from Huhan 3 based on the genetic backgrounds of Xihui 18. The grain width of Z414 was controlled by qGW5, and GS5 should be the candidate gene for qGW5 by sequencing and qRT-PCR analysis. The grain length of Z414 was controlled by qGL11, qGL3, and qGL5, and CycT1;3 should be the best candidate gene of qGL11, whose specific function of regulating grain length was still unknown, and qGL11 is epistatic to qGL3.
ZNF488 Enhances the Invasion and Metastasis through Activating the MAPK/ERK Signaling Pathway in Non-Small Cell Lung Cancer
Authors: Zhang Yu-feng , Zhu Huan-feng , Wang De-jun , Jiang Ning , Gu Jia-jia , Wu Li-rong , Zhao Li-jun , Chen Cheng , Guo Ye-song , Feng Ji-feng , Zong Dan , He Xia 
Published: September 2021

Abstract
Background: This study aimed to explore the relationship between zinc finger protein 488 (ZNF488) and non-small cell lung cancer (NSCLC), as well as the function and mechanism of ZNF488 involved in the invasion and metastasis. Method：TCGA and HPA databases were used to analyze NSCLC data. Quantitative Reverse transcription polymerase chain reaction (qRT-PCR) was used to detect the expression level of ZNF488 in tissues. 100 cases of NSCLC tissues were subjected to immunohistochemical staining (IHC) to evaluate the endogenous expression of ZNF488, and than analyze its correlation with clinical characteristics. After constructing ZNF488 stably overexpressing cell lines, wound healing test and transwell invasion assays were used to explore the invasion and migration ability of lung cancer cells in both ZNF488 over-expressing and the vector control cell lines. In vivo, we used ZNF488 over-expressing and the vector control cell lines to construct animal models of lung metastasis by tail vein injection. The gene chip technology was used to explore the differential genes between vector and ZNF488 over-expressing group. The DAVID database was used to analyze the differential genes by KEGG pathway MEK inhibitor AZD6244 was used to verify the potential signaling pathway. Western blot was used to detect the protein expression levels. Results：The expression of ZNF488 in NSCLC tissues was generally higher than that in normal lung tissues, and its expression level was significantly related to TNM stage, overall survival (OS), local recurrence-free survival (LRFS), distant metastasis-free survival (DMFS), and progression-free survival (PFS) (p<0.05). The results of functional and animal experiments showed that ZNF488 promoted the cell invasion and matastasis in NSCLC. In terms of mechanism, ZNF488 can induce epithelial-mesenchymal transition (EMT) in NSCLC by activating the MAPK/ERK signaling pathway, thereby promoting the invasion and metastasis. MEK inhibitor AZD6244. could partially reverse ZNF488-induced invasive ability. Conclusion: As an independent prognostic indicator, ZNF488 can promote the invasion and metastasis by activating the MAPK/ERK signaling pathway in NSCLC .
Long Term Implications in Surgical Re-assisting (L.I.S.A. study) During Covid-19 Outbreak: A Retrospective Observational Study on a Rural Population.
Authors: Fabbri Nicolò , Pesce Antonio , Uccellatori Lisa , Greco Salvatore , D'Urbano Francesco , Marchetti Francesco , Rubbini Michele , Feo Carlo V 
Published: September 2021

Abstract
BackgroundThe spread of the COVID-19 is having a worldwide impact on surgicaltreatment. Our aim was to investigate the impact of the pandemic in a rural hospital in a lowdensely populated area.MethodsWe investigated the volume and type of surgical operations during the pandemic(March 2020 - February 2021) versus pre-pandemic period (March 2019 - February 2020) aswell as during the first and second pandemic waves compared to the pre-pandemic period.We compared the volume and timing of emergency appendectomy and cholecystectomyduring the pandemic versus pre-pandemic period, the volume, timing and stages of electivegastric and colorectal resections for cancer during the pandemic versus the pre-pandemicperiod.ResultsIn the prepandemic versus pandemic period, 42 versus 24 appendectomies and 174versus 126 cholecystectomies (urgent and elective) were performed. Patients operated onbefore as opposed to during the pandemic were older (58 vs. 52 years old, p=0.006),including for cholecystectomy (73 vs. 66 years old, p=0.01) and appendectomy (43 vs. 30years old, p = 0.04).The logistic regression analysis with regard to cholecystectomy and appendectomy performedin emergency showed that male sex and age were both associated to gangrenous typehistology, both in pandemic and prepandemic period. Finally, we found a reduction in cancerstage I and IIA in pandemic versus prepandemic period, with no increase in the moreadvanced stages.Conclusionsthe reduction in services imposed by governments during the first months oftotal lock down did not justify the whole decrease in surgical interventions in the year of thepandemic. Data suggest that greater "non-operative management" for cases of appendicitisand acute cholecystitis does not lead to an increase in cases operated over time, nor to anincrease in the "gangrenous" pattern, which seems to depend on age advanced and malepopulation.
Characteristics of Macroalgal Consumption by Eight Herbivorous Coral Reef Fishes From the Xisha Islands, China, as Determined by Microscopy, 18S rRNA High-throughput Sequencing and Stable Isotope Analyses
Authors: Wu Peng , Wang Teng , Liu Yong , Li Chunhou , Xiao Yayuan , Xu Shannan , Han Tingting , Lin Lin , Quan Qiumei 
Published: September 2021

Abstract
Herbivorous fishes play an important role in controlling the overabundance of macroalgae on coral reefs. Understanding the feeding selectivity and consumption of macroalgae by herbivorous fishes can be challenging in studies of their ecological role in the preservation and recovery of coral reefs. Coral reef decline, macroalgal overgrowth and overfishing are clearly visible in the Xisha Islands, China. However, there have been no studies of the feeding behaviors of herbivorous fishes in this area. We used microscopy, 18S rRNA high-throughput sequencing and stable isotope analyses to comprehensively examine the dietary spectrum of eight herbivorous reef fish species common in the Xisha Islands, including one parrotfish, two chub, two unicorn fish and three rabbitfish. Multi-technique analyses of intestinal contents revealed that Kyphosus vaigiensis, Naso unicornis and Siganus argenteus showed a high consumption potential of macroalgae, suggesting that they are the key browsers which should receive priority protection in in the Xisha Islands. Kyphosus cinerascens, K. vaigiensis, N. unicornis and S. punctatissimus fed on the entire macroalgal thallus, indicating their greater ecological importance compared with species which only consume the algal fronds. However, Calotomus carolinus can consume the red alga Pneophyllum conicum, which is widely distributed on Indo-Pacific coral reefs and can overgrow and kill live corals. Clearly, a diverse herbivorous fish fauna is very important in the Xisha coral reefs. These results not only demonstrated the various functions of different herbivorous fish species in macroalgal removal, but also provided insights into the management of herbivorous fishes on the coral reefs of the South China Sea.
In vitro Gynogenic Plant Production in Allium tuncelianum (Kollman) Özhatay, Matthew &amp; Şiraneci
Authors: Celebi-Toprak Fevziye , Ergun Zeynep , Alan Ali R. 
Published: September 2021

Abstract
Allium tuncelianum (Kollman) Özhatay, Matthew & Şiraneci forms a single-cloved edible white bulb with mild garlic (A. sativum) odour and taste. Its ability to form seeds make it suitable for genetic improvement via classical and modern approaches. A detailed study was carried out to determine the gynogenesis potential of two A. tuncelianum (AT1 and AT2) accessions. Unopened flower buds of A. tuncelianum accessions were cultured in various BDS- and MS-based induction media. A total of 17 (0.09%) gynogenic plantlets were obtained from ~20000 flower buds used in gynogenesis induction experiment. Accessions showed slight differences in their responses to gynogenesis induction cultures. The highest gynogenic plantlet production frequency (0.34 %) in AT1 was achieved flower buds cultured in T12 medium (MS with 100 g/L sucrose, 1 mg/L a-naphthalene acetic acid (NAA) and 8 mg/L isopentenyl adenine (2IP). Flower buds of AT2 showed the highest gynogenic plantlet production response (0.44 %) in T2 medium (BDS with 50 g/L sucrose). Eight of 17 gynogenic plantlets continued to grow and became healthy plants with green leaves and well established roots. Flow cytometric (FCM) analysis of well-developed gynogenic plants showed that two were haploid (25 %), four were diploid (50 %), and two were mixoploid (25 %) for haploid and diploid cells. Nine gynogenic plantlets showing abnormal development were diploid. Therefore, formation of gynogenic plantlets with abnormal phenotypes was likely due to genetic factors. Results obtained from this study suggest use of DH technology in the production of homozygous A. tuncelianum inbreeds in variety improvement programs.
Natural History of Unruptured Vertebral Basilar Artery Dissection: Temporal Changes in Imaging Findings and Contributory Factors
Authors: masashi kuwabara , Sakamoto Shigeyuki , Okazaki Takahito , Mitsuhara Takafumi , Shimonaga Koji , Hosogai Masahiro , Maeda Yuyo , Kurisu Kaoru , Horie Nobutaka 
Published: September 2021

Abstract
PurposeTo investigate temporal changes in imaging findings of conservatively treated unruptured vertebral basilar artery dissection and its contributing factors.MethodsFifty-three patients who underwent conservative treatment for 64 cases of vertebral basilar artery dissection diagnosed between January 2006 and March 2019 and follow-up of at least 12 months after onset were retrospectively investigated. Statistical analyses of age, sex, medical history, pattern of onset, lesion site, imaging findings and changes over time, regular medication, and outcomes were performed. ResultsChanges in the vascular morphology of the lesion site during the follow-up period were observed in only 23 (43%) patients (median time until change: 19 days). Univariate analysis of factors contributing to morphological changes at the dissection site showed that changes were significantly more likely in younger patients (p = 0.011). Patients taking antiplatelet drugs had a significantly greater rate of deterioration at the dissection site (p = 0.028) than others. On multivariate analysis, age was an independent factor contributing to changes at the dissection site, and taking antiplatelet drugs, particularly clopidogrel, was an independent factor contributing to deterioration. No patient developed intracranial hemorrhage, cerebral infarction, or worsening of neurological symptoms during follow-up.ConclusionsMorphological changes at the dissection site are more likely in younger patients with unruptured vertebral basilar artery dissection and those taking antiplatelet drugs. However, chances of intracranial hemorrhage, cerebral infarction, or worsening of neurological symptoms during conservative therapy are low. Therefore, unruptured vertebral basilar artery dissection may be considered a benign condition.