bluuebunny/crossref_metadata_2025_split huggingface dataset:
WITHDRAWN: Transcriptional signatures associate with the clinical outcomes of immune checkpoint inhibitor treatment in advanced bladder cancer
Authors: Xu Xiaofeng , Fu Dian , Zhao Hai , Huang Jie , Wang Zuheng , Xu Feng , Chen Yuhao , Da Jianping , Zhang Tingling , Ge Jingping , Cheng Wen 
Published: October 2021

Abstract
Background
Urothelial bladder cancer (UBC) is one of the most lethal cancers worldwide, the 5-year survival rate remain poor with platinum-based chemotherapy regimens as the standard of cancer treatment protocol. Recent FDA approval of a programmed death ligand-1 (PD-L1) inhibitor, atezolizumab, in advanced UBC patients is changing the therapeutic landscape. Although the response to anti-PD-L1 is correlated to PD-L1 expression and tumor mutation burden, the molecule determinants of responsiveness or non-responsiveness to immune checkpoint inhibitor (ICI) is largely unknown.
Result
A published immunotherapy cohort with whole exome sequencing, RNAseq and clinic outcome data for 29 metastatic urothelial cancer patients was used, paralleled with The Cancer Genome Altas Bladder Cancer cohort for validation. Genomic mutational profiling, mutational signature, a panel genes in antigen presentation and interferon signaling in bladder cancer were delineated with little correlation with durable clinic benefit (DCB) or non-DCB of PD-L1 inhibitor treatment. Whereas, characterized immune-responsive or resistant associated genes showed differentially expressed between DCB group and non-DCB group. Further more, transcriptional signature and transcriptional regulators between DCB and non-DCB were identified from transcripome data.
Conclusion
Our exploratory analyses provided multidimension view of complexity of determinants of immune-responsiveness and suggest the influences of transcriptional reprogram in immune checkpoint blockage therapy.
A Wireless Body Area Sensor Based IOT Scheme for Healthcare Applications
Authors: C Anna Palagan , Gupta Sanjai , Dhas Anand J , Taware Shrikant , R Ravi Chakravarthi , A Ahilan 
Published: October 2021

Abstract
Between the collections of applications allowed by the IoT, smart and linked health care may be mainly vital one. Networked sensors, either damaged on body or entrenched in atmospheres, alter the assembly of wealthy info symptomatic of our physical and psychological health. For example, heart patient parameter such as BP, heart rate and activities of fetal to regulate their health state. In this paper, a coordinator node has devoted on patient’s body to gather all the signals from the wireless sensors and directs them to base station. The involved sensors on patient’s body form a WBAN and they are talented to sense the heart rate, BP and so on. This scheme can notice the irregular conditions, problem an alarm to the patient and direct a message to the clinician, ambulance and family. The focal benefit of this scheme in assessment to earlier systems is to decrease the energy consumption to extend the network period, speed up and encompass the statement coverage to upsurge the choice for enhance patient superiority of lifetime. Here, we focus the chances and tasks for WSN in understanding this idea of longer term of health care.
Involvement of MdWRKY40 in the Defense of Mycorrhizal Apple Against Fusarium Solani
Authors: Wang Mei , Tang Weixiao , Xiang Li , Chen Xuesen , Shen Xiang , Yin Chengmiao , Mao Zhiquan 
Published: October 2021

Abstract
Background: Apple (Malus domestica Borkh.) is an important economic crop. The pathological effects of Fusarium spp., a group of soilborne pathogens, on the root systems of apple plants was unknown. It was unclear how mycorrhizal apple seedlings resist infection by F. solani. The transcriptional profiles of mycorrhizal and non-mycorrhizal plants infected by F. solani were compared using RNA-Seq.Results: Infection with F. solani significantly reduced the dry weight of apple roots, and the roots of mycorrhizal apple plants were less damaged when the plants were infected with F. solani. They also had enhanced activity of antioxidant enzymes and a reduction in the oxidation of membrane lipids. A total of 1,839 differentially expressed genes (DEGs) were obtained after mycorrhizal and non-mycorrhizal apple plants were infected with F. solani. A gene ontogeny (GO) analysis showed that most DEGs were involved in the binding of ADP and calcium ions. In addition, the enrichment observed from an analysis with the Kyoto Encyclopedia of Genes and Genomes (KEGG) primarily involved plant-pathogen interaction and Glycolysis/Gluconeogenesis, the mitogen-activated protein kinase (MAPK) signaling pathway, and plant hormone signal transduction. Based on a MapMan analysis, an enormous number of DEGs were involved in the response of mycorrhizal plants to stress. Among them, the overexpressed transcription factor MdWRKY40 significantly improved the resistance of ‘Orin’ to F. solani and the expression of the resistance gene MdGLU by binding the promoter of MdGLU.Conclusion: This paper outlines how the inoculation of apple seedlings by arbuscular mycorrhizal fungi reduces the response to stress at the transcription level of the root system following infection with F. solani, and MdWRKY40 played an important role in the resistance of mycorrhizal apple seedlings to infection with F. solani.
Reduction of Testosterone Levels in Schistosoma Haematobium or Schistosoma Mansoni Infected Men: A Cross-Sectional Study in Two Schistosomiasis-Endemic Areas of the Adamawa Region of Cameroon
Authors: JATSA Hermine BOUKENG , FEMOE Ulrich MEMBE , DONGMO Calvine NOUMEDEM , KAMWA Romuald Issiaka NGASSAM , FESUH Betrand NONO , TCHUENTE Louis-Albert TCHUEM , KAMTCHOUING Pierre 
Published: October 2021

Abstract
Background: The incidence of schistosomiasis‐induced male reproductive dysfunction and infertility is probably underestimated comparing to female genital schistosomiasis. This study aimed to investigate the impact of S. haematobium or S. mansoni infection on the reproductive function of adult men in Tibati and Wouldé, two schistosomiasis endemic areas in the Adamawa region of Cameroon.Methods: A total of 89 men in the reproductive age (range: 14 – 56 years) from two localities were enrolled in the study with 51 in Tibati and 38 in Wouldé. Each participant was submitted to a questionnaire to document data on sociodemographic and stream contact behaviors. A medical examination was performed to measure the circumference of the testes and to evaluate genital tract pathologies. Stool and urine samples were collected and screened for the presence of S. haematobium or S. mansoni ova. Blood serum was use to evaluate the level of transaminases and testosterone.Results: S. haematobium was present only in Tibati with a prevalence of 31.37%. S. mansoni prevalence was 3.92% at Tibati and 44.71% in Wouldé. The intensity of infection was 22.12 ± 9.57 eggs/10 mL for S. haematobium and 128.10 ± 3.76 epg for S. mansoni. Serum transaminases activity and the mean testicular circumference of Schistosoma-positive individuals were close to those of negative ones. The testes size was however higher in S. mansoni-positive individuals than in S. haematobium-positive ones (P < 0.05). The serum testosterone level of S. haematobium and S. mansoni-positive men was significantly reduced by 56.07% (P < 0.001) and 51.94% (P < 0.01) respectively in comparison to that of Schistosoma-negative ones. A significant and negative correlation was established between schistosomiasis and the low level of serum testosterone. Male genital tract pathologies such as scrotal abnormalities, varicocele, nodular epididymis, inguinal hernia and hydrocele were recorded in both Schistosoma-positive and Schistosoma-negative men. However, no significant link was established between schistosomiasis infection and these pathologies.Conclusion: These results demonstrated that infection with S. haematobium or S. mansoni is associated with a low production of the reproductive hormone testosterone, and may be a major cause of male infertility.
The TCM Prescription Yi-Fei-Jie-Du-Tang Inhibit Invasive Migration and EMT of Lung Cancer Cells by Activating Autophagy
Authors: Wang Shanshan , Wang Zaichuan , Wu Yinqiu , Hou Chao , Wang Qingyin , Wu Yongjian , Qian Chao , Zhang Xiaochun 
Published: October 2021

Abstract
ObjectiveNon-small cell lung cancer (NSCLC) is a serious threat to people’s health. This study aims to assess the antitumor effect of Yi-Fei-Jie-Du-Tang (YFJDT) on NSCLC,which is an empirical formula from Professor Zhongying Zhou,and the underlying mechanisms.MethodsIn the present study, we determined the possible effects and potential mechanisms of YFJDT on an A549 cell tumor-bearing nude mice model and A549 cell model in vitro. Tumor-bearing mice were treated with YFJDT and tumors were measured during the experiment, and tumor tissues were collected at the end of the experiment to assess the levels of autophagy and epithelial-mesenchymal transition (EMT)-related proteins. ResultsThe results showed that YFJDT treatment reduced tumor volume and mass, increased the expression of the autophagy marker LC3 and inhibited EMT-related proteins compared to the model group.Cell survival was reduced in the YFJDT-treated group compared to the model group, and YFJDT also reduced the migration and invasion ability of A549 cells in a dose-dependent manner. Western blotting detected that YFJDT also upregulated FAT4 in tumor tissue and A549 cells and downregulated the expression of Vimentin. Meanwhile, apoptosis in both tissues and cells was greatly increased after YFJDT treatment. We further interfered with FAT4 expression in cells and found that the inhibitory effect of YFJDT on EMT was reversed, indicating that YFJDT affects EMT by regulating FAT4 expression. Conclusion﻿Taken together, results of this study suggested that the inhibitory effect of YFJDT on EMT in lung cancer tumors is through upregulating FAT4, promoting autophagy and thus inhibiting EMT in cancer cells.
Safety and Feasibility of the Transoral Endoscopic Thyroidectomy Vestibular Approach With Neuroprotection Techniques for Papillary Thyroid Carcinoma
Authors: Chen Zhen-Xin , Song Ya-Min , Chen Jing-Bao , Zhang Xiao-Bo , Pang Feng-Shun , Lin Zhan-Hong , Yang Li-Ming , Cai Bei-Yuan , Qin You 
Published: October 2021

Abstract
Background: This study aimed to evaluate the feasibility and safety of the trans-oral endoscopic thyroidectomy vestibular approach (TOETVA) with neuroprotection techniques for the surgical management of papillary thyroid carcinoma (PTC). Methods: Patients with PTC who underwent TOETVA between December 2016 and July 2020 were included in this study, and their relevant clinical characteristics, operational details, and surgical outcomes were reviewed and extracted from their medical records for further analysis. Results: A total of 75 patients successfully underwent TOETVA with zero conversions. Unilateral lobectomy with isthmectomy and total thyroidectomy were completed for 58 and 17 patients, respectively, all using our unique neuroprotective procedure and ipsilateral central neck dissection (CND). The mean number of retrieved lymph nodes versus positive lymph nodes was 6.8 ± 3.7 vs. 1.5 ± 2.3. Postoperative complications included three cases of transient superior laryngeal nerve (SLN) palsy (4.0%), five cases of transient recurrent laryngeal nerve (RLN) palsy (6.7%), 14 cases of transient hypoparathyroidism (18.7%), and two cases of lap perforation (2.7%). The follow-up period for patients with PTC lasted for 15.6 ± 10.9 months, during which no other complications or tumor recurrence were observed.Conclusion: TOETVA can be safely performed for patients with PTC with satisfactory results during the short-term follow-up period. Our neuroprotection techniques can be integrated into TOETVA, which is worth recommending for PTC patients who desire better cosmetic surgical outcomes.
A Stepwise Data Interpretation Process For Renal Amyloidosis Subtyping by LMD-MS
Authors: Ke Ming , Li Xin , Wang Lin , Yue Shuling , Zhao Beibei 
Published: October 2021

Abstract
Backgrounds: Systemic amyloidosis is classified according to the deposited amyloid protein, which determines its best therapeutic scheme. The laser microdissection combined with mass spectrometry (LMD-MS) technique is a promising approach for precise subtyping of amyloidosis, however, is hampered by how to interpret the MS data.Objectives: The objective of the present study is to establish a complete data interpretation procedure for LMD-MS based amyloidosis subtyping.Methods: Formalin fixed paraffin-embedded specimens from patients with renal amyloidosis were analyzed by LMD-MS for proteome quantification. Forty-two specimens were used for training the data interpretation procedure, which was validated by another 50 validation specimens. Area under receiver operating curve (AUROC) analysis of amyloid accompanying proteins (APOE, APOA4 and SAMP) for discriminating amyloidosis from non-amyloid nephropathies was performed.Results: A stepwise data interpretation procedure that include or exclude the subtypes group by group was established, in which, involvement of non-immunoglobulin amyloid protein is determined by P-score, involvement of immunoglobulin light chain is determined by variable of λ-κ, and immunoglobulin heavy chain’s participation is judged by H-score. This data interpretation method achieved a 88% accuracy in 50 validation specimens. The amyloid accompanying proteins showed significant quantitative differences between amyloidosis specimens and non-amyloid nephropathies. Each of the single accompanying protein had a AUROC value more than 0.9 for diagnosis of amyloidosis from non-amyloid control, and the averaged value of spectral count of the three accompanying proteins showed the highest AUROC (0.966), indicating it might be an alternative indicator for amyloidosis diagnosis.Conclusions: The proteomic data interpretation procedure for amyloidosis subtyping based on LMD-MS was established successfully, which has high clinical application value.
Self-diffusion coefficient and sound velocity of Fe-Ni-O fluid:
implications for the stratification of Earth’s outer core
Authors: Li Wei-Jie , Li Zi , Mo Chong-Jie , He Xian-Tu , Wang Cong , Zhang Ping 
Published: October 2021

Abstract
It is experimentally reported that the stratified layer atop Earth’s outer core is hundreds of kilometers thick with a maximum sound velocity reduction of 0.3% relative to the preliminary reference Earth model. However, why the sound velocity atop the outer core is reduced remains theoretically unclear. In this paper, the Ni and vital light O in the outer core were both considered to have implications for the stratification of Earth’s core, including the stratification thickness and the sound velocity profile. Ab initio molecular dynamics simulations were performed on the Fe-Ni-O fluid under the conditions of Earth’s outer core, and the self-diffusion coefficients and ion-ion dynamic structure factors were calculated. The self-diffusion coefficient of O is (19.56±0.83)×10-9 m2s-1 at the core-mantle boundary. Combining the diffusion equation with the time evolution of the O self-diffusion coefficient, the calculated stratification thickness at present is 194.7 km. The calculated ion-ion dynamic structural factors indicate that the sound velocity in the outmost outer core near the stratified layer is 7.86 km/s. These results show that Fe-Ni-O is a possible composition of the stratified layer atop the outer core featuring an appropriate thickness and a reduced sound velocity, thereby shedding light on the dynamic behavior of Earth’s core.
Boosting The Peripheral Immune Response in the Skeletal Muscles Improved Motor Function in ALS Transgenic Mice
Authors: Trolese Maria Chiara , Scarpa Carlotta , Melfi Valentina , Fabbrizio Paola , Sironi Francesca , Rossi Martina , Bendotti Caterina , Nardo Giovanni 
Published: October 2021

Abstract

Background: Monocyte chemoattractant protein 1 (MCP1/CCL2) is one of the most powerful pro-inflammatory chemokines. However, its signalling is pivotal in driving axonal and muscle regeneration following injury. We previously showed that MCP1 is strongly upregulated in the nervous system of slow-progressing than fast-progressing SOD1G93A mice, which are characterised by a poor immune response that leads to a massive nerve and muscle degeneration.Methods: To assess the MCP1-mediated therapeutic role, we boosted the chemokine along the motor unit of the two SOD1G93A ALS models through a single intramuscular injection of a scAAV9 vector engineered with the Mcp1 gene (scAAV9_MCP1) at the pre-symptomatic disease stage.Results: Our observations revealed that slow-progressing SOD1G93A mice responded positively to the scAAV9_MCP1 injection anticipating the activation of the immune response, which sustained the pro-regenerative programme within nerves and skeletal muscles, eventually slackening the symptoms progression. Conversely, fast-progressing SOD1G93A mice exhibited an adverse response to the treatment, exacerbating the toxic inflammatory response in the periphery, resulting in worsened motor ability late in the disease.Intriguingly, our data suggested a novel pleiotropic role of MCP1 in the nervous system of SOD1G93A mice capable of promoting axon regeneration and modulating neuroinflammation, with the overall effect of preventing neurodegeneration.Conclusions: We provided direct evidence underlying the pivotal role of the immune response in promoting and governing skeletal muscle regeneration and thus the speed of ALS progression. The comparison study performed in fast- and slow-progressing SOD1G93A mice spotlights the nature and temporal activation of the inflammatory response as limiting factors to protect the peripheral compartment and interfere with the disease course tangibly. Altogether, these observations highlight the immune response as a key determinant for disease variability and proffer a reasonable explanation for the failure of systemic immunomodulatory treatments suggesting new potential strategies to hamper ALS progression.
Interaction Between Historical Earthquakes and the 2021 Mw7.4 Maduo Event and Their Impacts on the Seismic Gap Areas Along the East Kunlun Fault
Authors: Dong Peiyu , Zhao Bin , Qiao Xuejun 
Published: October 2021

Abstract
On May 21, 2021 (UTC time), a Mw7.4 earthquake struck Maduo County, Qinghai Province, China. The rupture of this typical strike-slip event and its aftershocks along the Kunlun-Jiangcuo fault (JCF) propagated approximately 170 km from the epicenter. In this study, we calculated the coseismic and postseismic Coulomb stress changes induced by 14 historical earthquakes and investigated their impacts on the 2021 Maduo source area. We found that the JCF is in the stress shadow of these historical events with a combined ΔCFS range of approximately -0.4 to -0.2 MPa. Since the seismogenic fault of the 1937 event is nearly parallel and close to the JCF, the rupture of the 1937 event had the greatest inhibitory effect on Maduo source area. We hypothesize that the actual loading rate at the depth of the seismogenic layer in the Maduo source area is much higher than the simulated value (0.3 kPa/a). Consequently, the Maduo earthquake still occurred despite the considerable delaying effect of these historical earthquakes (especially the 1937 event). Our findings also indicate that the tectonic stress in the eastern Bayanhar block is still rapidly accumulating and adjusting. Our investigation further reveals the enhanced stress induced by the historical and Maduo events with ΔCFS values of approximately 30~300 kPa and 50~300 kPa on the XDS and the eastern end of the EKF, respectively, not only on the MMS but also at the eastern end of each branch segment of the EKF. Hence, considering the accumulation of tectonic stress, we suggest that the seismic hazard in these two regions has been promoted.
Prognostic Value and Immune Infiltration of ARMC10 in Pancreatic Adenocarcinoma Via Integrated Bioinformatics Analyses
Authors: Li Tianhao , Qin Xiaohan , Qin Cheng , Zhao Bangbo , Cao Hongtao , Yang Xiaoying , Wang Yuanyang , Li Zeru , Zhou Xington , Li Tianyu , Wang Weibin 
Published: October 2021

Abstract
Background: Armadillo repeat-containing 10 (ARMC10) is involved in the progression of multiple types of tumors. Pancreatic adenocarcinoma (PAAD) is a lethal disease with poor survival and prognosis.Methods: We acquired the data of ARMC10 in PAAD patients from the cancer genome atlas (TCGA) and gene expression omnibus (GEO) datasets and compared the expression level with normal pancreatic tissues. We evaluated the relevance between ARMC10 expression and clinicopathological factors, immune infiltration degree and prognosis in PAAD.Results: High expression of ARMC10 was relevant to T stage, M stage, pathologic stage, histologic grade, residual tumor, primary therapy outcome (P<0.05) and related to lower Overall-Survival (OS), Disease-Specific Survival (DSS), and Progression-Free Interval (PFI). Gene set enrichment analysis showed that ARMC10 was related to methylation in neural precursor cells (NPC), G alpha (i) signaling events, APC targets, energy metabolism, potassium channels and IL10 synthesis. The expression level of ARMC10 was positively related to the abundance of T helper cells and negatively to that of plasmacytoid dendritic cells (pDCs). Knocking down of ARMC10 could lead to lower proliferation, invasion, migration ability and colony formation rate of PAAD cells in vitro.Conclusions: Our research firstly discovered ARMC10 as a novel prognostic biomarker for PAAD patients and played a crucial role in immune regulation in PAAD.
Prevalence of malnutrition in School aged children, Kassala State, Sudan
Authors: Khalid Fatima Abbas Khalid , Eldirdery Muataz Mohamed Eldirdery , ElGasim Mamoun El-obeid ElGasim , Elhaj Malak Abd Elmajid Elhaj , Desogi Mohamed Abd Alrazik Desogi , Mukhtar Maowia Mohamed Mukhtar 
Published: October 2021

Abstract
Background : Childhood malnutrition is a major concern in developing countries due to high morbidity and mortality rate. To estimate the prevalence of malnutrition among school children, Kassala State, A cross-sectional study was conducted randomly among 2638 children. Height for age and body mass index for age z scores were calculated using WHO Anthro Plus software as indicators of stunting and thinness respectively. A multinomial logistic regression model was used to determine the associated factors. Results: The overall prevalence of stunted and thinness was 22.1% and 32.3% respectively. Stunting was significantly higher among children in age group 13-15 years old (OR 3.788; 95% CI: 2.802- 5.123), boys (OR 1.394; 95% CI: 1.114-1.743) and children from rural areas (OR 2.406; 95% CI: 1.768- 3.274). Likewise thinness was significantly higher among boys (OR 1.717; 95% CI: 1.436- 2.052), Rural residence (OR 1.885; 95% CI: 1.487- 2.389), child whose families depend on unsafe source of drinking water (OR 2.205; 95% CI: 1.484- 3.276). Moreover, children who skipped meal during the school day or bring their food from their houses OR 2.744; 95% CI: 2.131- 3.532, 1.752(95% CI: 1.441- 2.129) respectively). Maternal education and occupation, in addition to family size and child ordered had no effect on the prevalence of malnutrition. Conclusions: In the present study, different forms of malnutrition was documented and affected by child age, gender, residence, source of drinking water. Therefore, more attention is needed to focus on nutrition status, improving the school environment, maternal education, and regular health assessment.