bluuebunny/crossref_metadata_2025_split huggingface dataset:
A cell-free assay for rapid screening of inhibitors of hACE2-receptor - SARS-CoV-2-Spike binding
Authors: Kikuchi Nanami , Willinger Or , Granik Naor , Navon Noa , Ackerman Shanny , Samuel Ella , Antman Tomer , Katz Noa , Goldberg Sarah , Amit Roee 
Published: October 2021

ABSTRACTWe present a cell-free assay for rapid screening of candidate inhibitors of protein binding, focusing on inhibition of the interaction between the SARS-CoV-2 Spike receptor binding domain (RBD) and human angiotensin-converting enzyme 2 (hACE2). The assay has two components: fluorescent polystyrene particles covalently coated with RBD, termed virion-particles (v-particles), and fluorescently-labeled hACE2 (hACE2F) that binds the v-particles. When incubated with an inhibitor, v-particle - hACE2F binding is diminished, resulting in a reduction in the fluorescent signal of bound hACE2F relative to the non-inhibitor control, which can be measured via flow cytometry or fluorescence microscopy. We determine the amount of RBD needed for v-particle preparation, v-particle incubation time with hACE2F, hACE2F detection limit, and specificity of v-particle binding to hACE2F. We measure the dose response of the v-particles to a known inhibitor. Finally, we demonstrate that RNA-hACE2F granules trap v-particles effectively, providing a basis for potential RNA-hACE2F therapeutics.
Social media influencer marketing: the moderating role of materialism
Authors: Koay Kian Yeik , Cheung Man Lai , Soh Patrick Chin-Hooi , Teoh Chai Wen 
Published: October 2021

PurposeSocial media influencers (SMIs) have become an important source of influence that affects consumer behaviours in their decision-making processes. As such, this justifies scholarly attention in understanding how SMIs transfer their meanings to endorsed brands and drive consumers’ positive behavioural intentions. With the intention to fill this knowledge gap, this paper aims to examine the impact of SMIs’ credibility, as manifested by trustworthiness, attractiveness and expertise, along with the moderating effects of materialism, on followers’ purchase intention.Design/methodology/approachSelf-administrated online surveys were used to collect data from Instagram users. A total of 191 usable data were collected and analysed using partial least square structural equation modelling.FindingsThe results show that SMIs’ trustworthiness and expertise are significant predictors of followers’ purchase intention. Moreover, the moderating effect of materialism on the relationship between attractiveness and purchase intention is significant. Notably, the influence of attractiveness on purchase intention is greater when materialism is high.Originality/valueThis research contributes to the SMI literature by examining the influence of SMIs’ trustworthiness, attractiveness and expertise, along with the moderating effect of materialism, on followers’ purchase intention.
Future public policy guidelines for the sustainable development of Guaviare, Colombia for the year 2035
Authors: Camarena José Luis , Osorio Vera Francisco Javier , Rojas Jimenez Hector Heraldo , Borda Medina Ernesto , Esteban Torregroza Juan Camilo , Tabares-Valencia Jesús David 
Published: October 2021

PurposeThis paper aims to propose future public policy guidelines (FPPG) in sustainable regional development for Guaviare (Colombia) – a territory affected by environmental and social distress – for the year 2035.Design/methodology/approachFollowing collective action theory and sustainable regional development literature, a foresight exercise was conducted using site focus groups and semi-structured interviews with local participants to identify future strategic change drivers and the most relevant social actors for the attainment of economic, social and environmental development in the Guaviare through FPPG.FindingsThe findings suggest that the development of public policies regarding building consensus around Guaviare’s economic, environmental and social issues, reducing conflict between the region’s cultural and environmental ways, decreasing isolation from the centers of decision-making, increasing the transparency of public institutions and reducing insecurity to attract investments are all crucial to attaining sustainable regional development.Originality/valueInterdisciplinarity is implicit in the local perspectives on the problem that impedes sustainable development in San José del Guaviare. The paper’s main contribution is the long-term vision that breaks away from the traditional short-termism in public policy guidelines in a Latin American context. Methodologically, the significant contribution is the convergent alignment of specific foresight methods toward public policy guidelines’ analysis and design processes.
An analogy between analytical, approximate and numerical methods in nonlinear buckling of functionally graded columns
Authors: Salmalian Kaveh , Alijani Ali , Ramezannejad Azarboni Habib 
Published: October 2021

Purpose
The purpose of this study is to investigate the post-buckling analysis of functionally graded columns by using three analytical, approximate and numerical methods. A pre-defined function as an initial assumption for the post-buckling path is introduced to solve the differential equation. The finite difference method is used to approximate the lateral deflection of the column based on the differential equation. Moreover, the finite element method is used to derive the tangent stiffness matrix of the column.

Design/methodology/approach
The non-linear buckling analysis of functionally graded materials is carried out by using three analytical, finite difference and finite element methods. The elastic deformation and Euler-Bernoulli beam theory are considered to establish the constitutive and kinematics relations, respectively. The governing differential equation of the post-buckling problem is derived through the energy method and the calculus variation.

Findings
An incremental iterative solution and the perturbation of the displacement vector at the critical buckling point are performed to determine the post-buckling path. The convergence of the finite element results and the effects of geometric and material characteristics on the post-buckling path are investigated.

Originality/value
The key point of the research is to compare three methods and to detect error sources by considering the derivation process of relations. This comparison shows that a non-incremental solution in the analytical and finite difference methods and an initial assumption in the analytical method lead to an error in results. However, the post-buckling path in the finite element method is traced by the updated tangent stiffness matrix in each load step without any initial limitation.
Lessons learned from the impact of COVID-19 on the global construction industry
Authors: Ogunnusi Mercy , Omotayo Temitope , Hamma-Adama Mansur , Awuzie Bankole Osita , Egbelakin Temitope 
Published: October 2021

PurposeThe construction industry represents most of every country’s finances and vital to continued economic growth and activities, especially in developing countries. The impact of the severe acute respiratory syndrome-2 disease (COVID19) on the government’s income resulted in the expectation of many public projects being cancelled or delayed providing little opportunity for the emergence of new public projects. This study collated a global qualitative perspective (survey interviews) on the lessons learned during the COVID-19 pandemic and the positive and negative impacts for future-proofing the construction sector.Design/methodology/approachIn total, 76 respondents from five continents excluding South America responded to the online open-ended structured questionnaire. Data collected were analysed through artificial inteligence analytics tool – Zoho analytics.FindingsThe themes indicating the positive impact obtained from the interview were overhead cost reduction, remote working environment, focus on health and safety, improved productivity and sustainability goals while the themes signifying the negative impact were low business turnover, delays in construction payment and output, difficulties working from home and job losses. Supply chain management, construction project management improvement, concentration on health and safety and effective virtual working environment were collated as themes on lessons learned.Social implicationsThe major findings of this study emphasise on the need to improve the occupational health and safety and onsite safety measures for future proofing of the construction industry.Originality/valueThe findings from the analyses made clear the imperativeness of the built environment research, with a focus on novel framework and strategies for future proofing the construction industry.
“I didn’t want to be a troublemaker” – doctoral students’ experiences of change in supervisory arrangements
Authors: Schmidt Manuela , Hansson Erika 
Published: October 2021

Purpose
During the lengthy process of PhD studies, supervisory changes commonly occur for several different reasons, but their most frequent trigger is a poor supervisory relationship. Even though a change in supervisors is a formal bureaucratic process and not least the students’ rights, in practice it can be experienced as challenging. Therefore, the purpose of this study is to explore how doctoral students experience a change in supervisory arrangements.

Design/methodology/approach
This study highlights the voices of 19 doctoral students who experienced at least one supervisory change during their doctoral studies.

Findings
The findings were structured chronologically, revealing the students’ experiences prior, during and after the changes. In total, 12 main themes were identified. Most of the interviewed students experienced the long decision-making processes as stressful, difficult and exhausting, sometimes causing a lack of mental well-being. However, once the change was complete, they felt renewed, energized and capable of continuing with their studies. It was common to go through more than one change in supervisory arrangements. Further, the students described both the advantages of making a change yet also the long-lasting consequences of this change that could affect them long after they had completed their PhD programs.

Originality/value
The study fulfills an identified need to investigate the understudied perspective of doctoral students in the context of change in supervisory arrangements. A change in the academic culture is needed to make any changes in supervisory arrangements more acceptable thus making PhD studies more sustainable.
Effect of NPC1L1 polymorphism on warfarin stable dose in Chinese patients under heart valve replacement surgery
Authors: Ma Siqing , Luo Zhiying , Zhou Xinmin , Zhou Honghao , Chen Ling , Zhang Wei 
Published: October 2021

AbstractWarfarin is the most often anticoagulant choice for preventable thromboembolism. Notably, vitamin K plays a vital role in the process of warfarin's anticoagulant effect. Therefore, we presume NPC1L1, a key transporter of vitamin K (VK) intestinal absorption, may modulate the anticoagulant effect of warfarin. Studies have shown that NPC1L1(‐762T>C, rs2073548) and p53 (P72R, rs1042522) variations are implicated in influencing NPC1L1 expression. This study aimed to assess the association between these two variants and warfarin stable dose (WSD). A two‐stage extreme phenotype design was used to explore the influence of these two variants (rs2073548, rs1042522) on WSD variance in 655 Chinese patients undergoing heart valve replacement surgery. NPC1L1 rs2073548, p53 rs1042522, VKORC1 rs9923231 and CYP2C9*1/*3 polymorphisms were genotyped by polymerase chain reaction‐restriction fragment polymorphism (PCR‐RFLP) or Sanger sequencing, respectively. WSD was identified when target monitoring international normalized ratio (INR) value at 2.0‐3.0. In the discovery phase, NPC1L1 rs2073548 A allele carriers occupied a significantly higher rate in the low dose group (P = .019). However, in the validation group, warfarin dosage in patients with the rs2073548 AA, AG and GG genotypes were 2.91 ± 0.97 mg/day, 3.02 ± 1.00 mg/day and 3.00 ± 1.06 mg/day, respectively. Multiple linear regression analysis results suggested that CYP2C9*3 and VKORC1 rs9923231, but not NPC1L1 rs2073548, were independent predictors of WSD in Chinese heart valve replacement (HVR) surgical patients.
In defense of Direct Care: Limiting access to military hospitals could worsen quality and safety
Authors: Zogg Cheryl K. , Lichtman Judith H. , Dalton Michael K. , Learn Peter A. , Schoenfeld Andrew J. , Perez Koehlmoos Tracey , Weissman Joel S. , Cooper Zara 
Published: October 2021

AbstractObjectiveOngoing health care reforms within the US Military Health System (MHS) are expected to shift >1.9 million MHS beneficiaries from military treatment facilities (MTFs) into local civilian hospitals over the next 1–2 years. The objective of this study was to examine how such health care reforms are likely to affect the quality of MHS care.Data sourcesAdult MHS beneficiaries, aged 18–64 years, treated in MTFs (under a program known as Direct Care) were compared against (1) MHS beneficiaries treated in locally available civilian hospitals (under a program known as Purchased Care) and (2) similarly‐aged adult civilian patients across the United States. MHS beneficiaries in Direct and Purchased Care were identified from fiscal‐year 2016–2018 MHS inpatient claims. National inpatients were identified in the 2017 Nationwide Readmissions Database.Study designRetrospective cohort.Data collectionDifferences in quality were compared using two sets of quality metrics endorsed by the US Agency for Healthcare Research and Quality (AHRQ): Inpatient Quality Indicators, 19 quality metrics that look at differences in in‐hospital mortality, and Patient Safety Indicators, 18 quality metrics that look at differences in in‐hospital morbidity and adverse events. Among MHS beneficiaries (Direct and Purchased Care), we further simulated what changes in quality indicators might look like under various proposed scenarios of reduced access to Direct Care.Principal findingsA total of 502,252 MHS admissions from 37 MTFs and surrounding civilian hospitals were included (326,076 Direct Care, 179,176 Purchased Care). Nationwide, 9.34 million adult admissions from 2453 hospitals were included. On average, MHS beneficiaries treated in MTFs experienced better inpatient quality and improved patient safety compared with MHS beneficiaries treated in locally available civilian hospitals (e.g., summary observed‐to‐expected ratio for medical mortality: 0.98 vs. 1.03, p < 0.001) and adult patients across the United States (0.98 vs. 1.02, p < 0.001). Simulations of proposed changes resulted in consistently worse outcomes for MHS patients, whether reducing MTF access by 10%, 20%, or 50% nationwide; limiting MTF access to active‐duty beneficiaries; or closing MTFs with the worst performance on patient safety (p < 0.001 for overall quality indicators for each).ConclusionsReducing access to MTFs could result in significant harm to MHS patients. The results underscore the importance of health‐policy planning based on evidence‐based evaluation and the need to consider the consequential downstream effects caused by changes in access to care.
Multispecies site occupancy modelling and study design for spatially replicated environmental DNA metabarcoding
Authors: Fukaya Keiichi , Kondo Natsuko Ito , Matsuzaki Shin‐ichiro S. , Kadoya Taku 
Published: October 2021

Abstract

Environmental DNA (eDNA) metabarcoding has become widely applied to gauge biodiversity in a non‐invasive and cost‐efficient manner. The detection of species using eDNA metabarcoding is, however, imperfect owing to various factors that can cause false negatives in the inherent multistage workflow. Imperfect detection in the multistage workflow of eDNA metabarcoding also raises an issue of study design, namely, how available resources should be allocated among the different stages to optimize survey efficiency.

Here, we propose a variant of the multispecies site occupancy model for eDNA metabarcoding studies where samples are collected at multiple sites within a region of interest. Unlike traditional site occupancy models that deal with binary detection data, this model describes the variation in sequence reads, the output of the high‐throughput sequencers. It explicitly accounts for the hierarchical workflow of eDNA metabarcoding and interspecific heterogeneity and allows the analysis of the sources of variation in the detectability of species throughout the different stages of the workflow. We also introduced a Bayesian decision analysis framework to identify the study design that optimizes the effectiveness of species detection with a limited budget.

An application of the model to freshwater fish communities in the Lake Kasumigaura watershed, in Japan, highlighted a remarkable inhomogeneity in the detectability of species, indicating a potential risk of the biased detection of specific species. Species with lower site occupancy probabilities tended to be difficult to detect as they had lower capture probabilities and fewer sequence reads. The expected abundance of sequence reads was predicted to vary by up to 23.5 times among species. An analysis of the study design suggested that ensuring multiple within‐site replications of the environmental samples is preferred to achieve higher species detection effectiveness, provided that tens of thousands of sequence reads were secured per replicate.

The proposed framework makes the application of eDNA metabarcoding more error‐tolerant, allowing ecologists to monitor ecological communities more efficiently.
Impact of single‐stage laparoscopic trans‐cystic exploration on hospital procedures, admissions and length‐of‐stay in common bile duct stone clearance
Authors: Vu Anh N. , Eskander Jacqueline , Chan Steven T. F. , Houli Nezor , Bui Hai T. 
Published: October 2021

AbstractBackgroundSingle‐stage approach with bile duct exploration is considered the most efficient and cost‐effective method of bile duct clearance. In Australia, apart from centres with subspecialty interests, notably in Brisbane, Queensland, a multi‐stage approach with endoscopic retrograde cholangiopancreatography (ERCP) is used more frequently. We aim to evaluate the impact of single stage laparoscopic trans‐cystic exploration (LTCE) versus multi‐stage approach for choledocholithiasis.MethodsThis was a retrospective cohort study. Medicare Benefits Schedule codings were used to identify patients who had the following procedures between December 2011 and December 2019: laparoscopic cholecystectomy (LC) and ERCP, LC and LTCE, LC and LTCE and ERCP. Primary outcomes were number of hospital procedures, admissions and additive length of stay (aLOS), the cumulative hospital stay from admission to discharge.ResultsOf 607 patients, 204 (34%) patients received a single‐stage LTCE, while 403 (66%) patients had a multi‐stage approach. In the LTCE group, 82% (168) patients and 93% (190) patients had one procedure and one admission respectively for stone clearance (P = 0.001). The median aLOS was 4 days for LTCE versus 7 days for multi‐stage approach (P = 0.001; 95% CI for difference − 3 to −2). In the multi‐stage group, 16% (65) patients had three or more procedures and 49% (199) patients required two or more hospital admissions to achieve stone clearance.ConclusionLTCE for stone clearance can be successfully accomplished with reductions in hospital admissions, number of procedures and length of stay. This has further economic and health resource implications.
Genetic alteration and clonal evolution of primary glioblastoma into secondary gliosarcoma
Authors: Li Jie , Zhao Yu‐Hang , Tian Su‐Fang , Xu Cheng‐Shi , Cai Yu‐Xiang , Li Kai , Cheng Yan‐Bing , Wang Ze‐Fen , Li Zhi‐Qiang 
Published: October 2021

AbstractAimsSecondary gliosarcoma (SGS) rarely arises post treatment of primary glioblastoma multiforme (GBM), and contains gliomatous and sarcomatous components. The origin and clonal evolution of SGS sarcomatous components remain uncharacterized. Therapeutic radiation is mutagenic and can induce sarcomas in patients with other tumor phenotypes, but possible causal relationships between radiotherapy and induction of SGS sarcomatous components remain unexplored. Herein, we investigated the clonal origin of SGS in a patient with primary GBM progressing into SGS post‐radiochemotherapy.MethodsSomatic mutation profile in GBM and SGS was examined using whole‐genome sequencing and deep‐whole‐exome sequencing. Mutation signatures were characterized to investigate relationships between radiochemotherapy and SGS pathogenesis.ResultsA mutation cluster containing two founding mutations in tumor‐suppressor genes NF1 (variant allele frequency [VAF]: 50.0% in GBM and 51.1% in SGS) and TP53 (VAF: 26.7% in GBM and 50.8% in SGS) was shared in GBM and SGS. SGS exhibited an overpresented C>A (G>T) transversion (oxidative DNA damage signature) but no signature 11 mutations (alkylating‐agents – exposure signature). Since radiation induces DNA lesions by generating reactive oxygen species, the mutations observed in this case of SGS were likely the result of radiotherapy rather than chemotherapy.ConclusionsSecondary gliosarcoma components likely have a monoclonal origin, and the clone possessing mutations in NF1 and TP53 was likely the founding clone in this case of SGS.
Can a Robot Lie? Exploring the Folk Concept of Lying as Applied to Artificial Agents
Authors: Kneer Markus 
Published: October 2021

AbstractThe potential capacity for robots to deceive has received considerable attention recently. Many papers explore the technical possibility for a robot to engage in deception for beneficial purposes (e.g., in education or health). In this short experimental paper, I focus on a more paradigmatic case: robot lying (lying being the textbook example of deception) for nonbeneficial purposes as judged from the human point of view. More precisely, I present an empirical experiment that investigates the following three questions: (a) Are ordinary people willing to ascribe deceptive intentions to artificial agents? (b) Are they as willing to judge a robot lie as a lie as they would be when human agents engage in verbal deception? (c) Do people blame a lying artificial agent to the same extent as a lying human agent? The response to all three questions is a resounding yes. This, I argue, implies that robot deception and its normative consequences deserve considerably more attention than they presently receive.
Language Processing Differences Between Blind and Sighted Individuals and the Abstract Versus Concrete Concept Difference
Authors: Canessa Enrique , Chaigneau Sergio E. , Moreno Sebastián 
Published: October 2021

AbstractIn the property listing task (PLT), participants are asked to list properties for a concept (e.g., for the concept dog, “barks,” and “is a pet” may be produced). In conceptual property norming (CPNs) studies, participants are asked to list properties for large sets of concepts. Here, we use a mathematical model of the property listing process to explore two longstanding issues: characterizing the difference between concrete and abstract concepts, and characterizing semantic knowledge in the blind versus sighted population. When we apply our mathematical model to a large CPN reporting properties listed by sighted and blind participants, the model uncovers significant differences between concrete and abstract concepts. Though we also find that blind individuals show many of the same processing differences between abstract and concrete concepts found in sighted individuals, our model shows that those differences are noticeably less pronounced than in sighted individuals. We discuss our results vis‐a‐vis theories attempting to characterize abstract concepts.