bluuebunny/crossref_metadata_2025_split huggingface dataset:
Increases in depression and anxiety symptoms in adolescents and young adults during the COVID-19 pandemic
Authors: Hawes Mariah T. , Szenczy Aline K. , Klein Daniel N. , Hajcak Greg , Nelson Brady D. 
Published: January 2021

AbstractBackgroundThe coronavirus [coronavirus disease 2019 (COVID-19)] pandemic has introduced extraordinary life changes and stress, particularly in adolescents and young adults. Initial reports suggest that depression and anxiety are elevated during COVID-19, but no prior study has explored changes at the within-person level. The current study explored changes in depression and anxiety symptoms from before the pandemic to soon after it first peaked in Spring 2020 in a sample of adolescents and young adults (N = 451) living in Long Island, New York, an early epicenter of COVID-19 in the U.S.MethodsDepression (Children's Depression Inventory) and anxiety symptoms (Screen for Child Anxiety Related Symptoms) were assessed between December 2014 and July 2019, and, along with COVID-19 experiences, symptoms were re-assessed between March 27th and May 15th, 2020.ResultsAcross participants and independent of age, there were increased generalized anxiety and social anxiety symptoms. In females, there were also increased depression and panic/somatic symptoms. Multivariable linear regression indicated that greater COVID-19 school concerns were uniquely associated with increased depression symptoms. Greater COVID-19 home confinement concerns were uniquely associated with increased generalized anxiety symptoms, and decreased social anxiety symptoms, respectively.ConclusionsAdolescents and young adults at an early epicenter of the COVID-19 pandemic in the U.S. experienced increased depression and anxiety symptoms, particularly amongst females. School and home confinement concerns related to the pandemic were independently associated with changes in symptoms. Overall, this report suggests that the COVID-19 pandemic is having multifarious adverse effects on the mental health of youth.
Different Prevention and Treatment Strategies for Knee Osteoarthritis (KOA) with Various Lower Limb Exoskeletons – A Comprehensive Review
Authors: Zhou Xin , Liu Geng , Han Bing , Li Hui , Zhang Li , Liu Xiaoli 
Published: January 2021

SUMMARYIt was reported that about 10% of people suffer from painful knee arthritis, and a quarter of them were severely disabled. The core activities of daily living were severely limited by knee osteoarthritis (KOA). In order to reduce knee pain and prolong the life of the knee joint, there has been an increasing demand on the development of exoskeletons, for prevention and treatment. The course of KOA was closely related to the biomechanics of knee joint, and the pathogenesis was summarized based on the biomechanics of knee joint. For the prevention and clinical treatment, exoskeletons are classified into three categories: prevention, treatment, and rehabilitation after the operation. Furthermore, the design concepts, actuators, sensors, control strategies, and evaluation criteria were presented. Finally, the shortcomings and limitations were summarized. It is useful for researchers to develop suitable exoskeletons in the future.
Integration of conflict resolution automation and vertical situation display for on-ground air traffic control operations
Authors: Trapsilawati Fitri , Chen Chun-Hsien , Wickens Chris D. , Qu Xingda 
Published: January 2021

AbstractBoth conflict resolution aid (CRA) and vertical situation display (VSD) systems may contribute to air traffic control (ATC) operations. However, their effectiveness still needs to be examined before being widely adopted in ATC facilities. This study aims to examine empirically the use of CRA and VSD as well as the systems’ interaction in ATC operations. It was found that CRA benefited conflict resolution performance by 13⋅7% and lowered workload by 46⋅4% compared with manually performing the task. The VSD could also reduce the air traffic controllers’ (ATCOs) workload and improve their situation awareness. Ultimately, when the first CRA failure occurred, the situation awareness supported by VSD offset the performance decrements by 30%. The findings from this study demonstrate that integrating VSD with CRA would benefit ATC operations, regardless of the CRA's imperfection.
Ecological and environmental factors affecting the foraging activity of the White-bellied Heron Ardea insignis (Hume, 1878) in Bhutan
Authors: KHANDU PEMA , GALE GEORGE A. , BUMRUNGSRI SARA 
Published: January 2021

SummaryWhite-bellied Heron Ardea insignis (WBH) is critically endangered, but we lack data on many aspects of its basic ecology and threats to the species are not clearly understood. The goal of this study was to analyse WBH foraging microhabitat selection, foraging behaviour, and prey preferences in two river basins (Punatsangchhu and Mangdechhu) in Bhutan which are likely home to one of the largest remaining populations of WBH. We also explored the relationship between the relative abundance of the WBH and prey biomass catch per unit effort within four foraging river microhabitats (pool, pond, riffle and run). Prey species were sampled in 13 different 100-m thalweg lengths of the rivers using cast nets and electrofishing gear. Riffles and pools were the most commonly used microhabitats; relative abundance was the highest in riffles. The relative abundance of WBH and prey biomass catch per unit effort (CPUE) also showed a weak but significant positive correlation (rs = 0.22). The highest biomass CPUE was observed in riffles while the lowest was found in the ponds. From the 97 prey items caught by the WBH, 95% of the prey were fish. The WBH mainly exploited three genera of fish (Garra, Salmo, and Schizothorax) of which Schizothorax (64%) was the most frequently consumed. This study provides evidence in support of further protection of critical riverine habitat and fish resources for this heron. Regular monitoring of sand and gravel mining, curbing illegal fishing, habitat restoration/mitigation, and developing sustainable alternatives for local people should be urgently implemented by the government and other relevant agencies. Further study is also required for understanding the seasonal variation and abundance of its prey species in their prime habitats along the Punatsangchhu and Mangdechhu basins.
Embryonic development of the fire-eye-tetra Moenkhausia oligolepis (Characiformes: Characidae)
Authors: dos Santos Raquel Santos , Rodrigues Jeane Rodrigues , Cordeiro Jhennifer Gomes , Tercya Hadda , Leite Marissol , Costa Bruna Patrícia Dutra , da Silva Costa Raphael , Maximino Caio , Siqueira-Silva Diógenes Henrique de 
Published: January 2021

SummaryThis study describes the embryonic development of Moenkhausia oligolepis in laboratory conditions. After fertilization, the embryos were collected every 10 min up to 2 h, then every 20 min up to 4 h, and afterwards every 30 min until hatching. The fertilized eggs of M. oligolepis measured approximately 0.85 ± 0.5 mm and had an adhesive surface. Embryonic development lasted 14 h at 25ºC through the zygote, cleavage, blastula, gastrula, neurula, and segmentation phases. Hatching occurred in embryos around the 30-somites stage. The present results contribute only the second description of embryonic development to a species from the Moenkhausia genus, being also the first for this species. Such data are of paramount importance considering the current conflicting state of this genus phylogenetic classification and may help taxonomic studies. Understanding the biology of a species that is easily managed in laboratory conditions and has an ornamental appeal may assist studies in its reproduction to both supply the aquarium market and help the species conservation in nature. Moreover, these data enable the use of M. oligolepis as a model species in biotechnological applications, such as the germ cell transplantation approach.
Perioperative and long-term management of Fontan patients
Authors: Al-Shawk May , Banjoko Adeolu , Axiaq Ariana , Amin Kiran , Harky Amer 
Published: January 2021

AbstractA Fontan circulation requires a series of three-staged operations aimed to palliate patients with single-ventricle CHD. Currently, the most frequent technique is the extracardiac total cavopulmonary connection, an external conduit connecting the IVC and right pulmonary artery, bypassing the right side of the heart. Fontan candidates must meet strict criteria; they are assessed utilising both cardiac catheterisation and cardiac magnetic resonance. Postoperatively, treatment protocols prioritise antibiotic prophylaxis, diuretics, angiotensin-converting enzyme inhibitors, anticoagulation, and oxygen therapy with fluid restriction and a low-fat diet. These measures aim to reduce length of stay in the ICU and hospital by preventing acute complications such as infection, venous thromboembolism, low cardiac output, pleural effusion, and acute kidney injury. Late complications of a Fontan procedure include circulation failure, protein-losing enteropathy, plastic bronchitis, and Fontan-associated liver disease. The definitive management is cardiac transplantation, with promising innovations in selective embolisation of lymphatic vessels and Fontan-specific ventricular assist devices. Further research assessing current protocols in the perioperative management of Fontan patients would be beneficial for standardising current practice and improving outcomes.
Fancy Footwork: Furniture Patterns Going to and from Gandhāra
Authors: SRINIVASAN DORIS METH 
Published: January 2021

AbstractThis paper is the first assessment of the different sources and shapes in a selection of Gandhāran furniture. Two main types of furniture legs are discussed. These are thought to be originally wooden legs attached to chairs, stools, and beds that are depicted in Kushan sculpture from Gandhāran. The two types are defined by their decoration. The first type is the geometric, lathe-turned furniture leg, the second type of leg is carved, not lathe-turned and depicts figures. The foreign origins of both types are traced and analyzed. A list, plus illustrations of reliefs showing representative contexts for each type, is given. Several heretofore unpublished reliefs are included. In some cases the Gandhāran examples can be interpreted as well as their developments into Central Asian furniture types.
NEW CONSTRUCTIONS OF SELF-COMPLEMENTARY CAYLEY GRAPHS
Authors: LI CAI HENG , RAO GUANG , SONG SHU JIAO 
Published: January 2021

AbstractVertex-primitive self-complementary graphs were proved to be affine or in product action by Guralnicket al.[‘On orbital partitions and exceptionality of primitive permutation groups’,Trans. Amer. Math. Soc.356(2004), 4857–4872]. The product action type is known in some sense. In this paper, we provide a generic construction for the affine case and several families of new self-complementary Cayley graphs are constructed.
A PHILOSOPHER WORKS FROM HOME: DIARIES OF A PHILOSOPHICAL ROMP THROUGH VAST TERRITORIES, SOMETIMES CROSSING OVER INTO UNCHARTED REGIONS OCCUPIED BY OTHER PEOPLE
Authors: Thalos Mariam 
Published: January 2021

ABSTRACTThis is my impersonation of a philosopher working from home, which aims at making lively a few worthy philosophical questions. The old is new again, as each generation confronts its own challenges and demons, in its own context.
The difference a wire makes: planning law, public Orthodox Judaism and urban space in Australia
Authors: Riedel Mareike 
Published: January 2021

AbstractThis paper considers a planning dispute that surrounded the construction of a Jewish religious installation (called an eruv) in the public urban space of an Australian suburb. The aim of this case-study is to examine the role of law in regulating Jewish difference – a topic that has to date received little attention in the socio-legal literature concerned with the governance of religious diversity. In analysing residents’ objections to the eruv, the paper explores long-standing anxieties about Jewish particularity in Australia and beyond as they surfaced in opposition to the eruv. It shows how the law continues to exclude certain forms of Jewish difference that are perceived as transgressing dominant religious and racial norms. Moreover, the paper highlights the particular ways in which planning law assigned value to these anxieties and legitimised the marginalisation of Orthodox Jews, emphasising the significance of local law as a site for exclusion and inequality.
The Paradox of the Thai Middle Class in Democratisation
Authors: Lertchoosakul Kanokrat 
Published: January 2021

AbstractThe relationship of the bourgeoisie and democratisation has been inconsistent across the history of democracy. This work offers an alternative explanation taking the example of the Thai middle class, which had promoted democracy, turned against it. From the democratic transition of 1973 until the present day, the Thai middle class has played contradictory roles in the democratisation of the country. This work investigates the effects of democratic institution-building after regime change and the efforts to consolidate democracy in the middle class. This work proposes two major observations. The first is the failure of the middle class to establish themselves in democratic institutions and processes in either the legislature/executive, political parties, local government or structured interest groups. They have learned of the uncertainty of free elections and how the elected executives have benefitted other classes but not them. The second regards the missing prerequisite of democracy. Insufficient understanding of majority rules and two-turnover elections, caused the middle class who were disappointed with the outcome of democratic regimes and systems to easily turn away from democracy.
Serotonin receptor 4 in the hippocampus modulates mood and anxiety
Authors: Karayol Remzi , Medrihan Lucian , Warner-Schmidt Jennifer L. , Fait Ben W. , Rao Meghana N. , Holzner Eva B. , Greengard Paul , Heintz Nathaniel , Schmidt Eric F. 
Published: January 2021

AbstractSerotonin receptor 4 (5-HT4R) plays an important role in regulating mood, anxiety, and cognition, and drugs that activate this receptor have fast-acting antidepressant (AD)-like effects in preclinical models. However, 5-HT4R is widely expressed throughout the central nervous system (CNS) and periphery, making it difficult to pinpoint the cell types and circuits underlying its effects. Therefore, we generated a Cre-dependent 5-HT4R knockout mouse line to dissect the function of 5-HT4R in specific brain regions and cell types. We show that the loss of functional 5-HT4R specifically from excitatory neurons of hippocampus led to robust AD-like behavioral responses and an elevation in baseline anxiety. 5-HT4R was necessary to maintain the proper excitability of dentate gyrus (DG) granule cells and cell type-specific molecular profiling revealed a dysregulation of genes necessary for normal neural function and plasticity in cells lacking 5-HT4R. These adaptations were accompanied by an increase in the number of immature neurons in ventral, but not dorsal, dentate gyrus, indicating a broad impact of 5-HT4R loss on the local cellular environment. This study is the first to use conditional genetic targeting to demonstrate a direct role for hippocampal 5-HT4R signaling in modulating mood and anxiety. Our findings also underscore the need for cell type-based approaches to elucidate the complex action of neuromodulatory systems on distinct neural circuits.
Prenatal androgen exposure causes a sexually dimorphic transgenerational increase in offspring susceptibility to anxiety disorders
Authors: Risal Sanjiv , Manti Maria , Lu Haojiang , Fornes Romina , Larsson Henrik , Benrick Anna , Deng Qiaolin , Cesta Carolyn E. , Rosenqvist Mina A. , Stener-Victorin Elisabet 
Published: January 2021

AbstractIf and how obesity and elevated androgens in women with polycystic ovary syndrome (PCOS) affect their offspring’s psychiatric health is unclear. Using data from Swedish population health registers, we showed that daughters of mothers with PCOS have a 78% increased risk of being diagnosed with anxiety disorders. We next generated a PCOS-like mouse (F0) model induced by androgen exposure during late gestation, with or without diet-induced maternal obesity, and showed that the first generation (F1) female offspring develop anxiety-like behavior, which is transgenerationally transmitted through the female germline into the third generation of female offspring (F3) in the androgenized lineage. In contrast, following the male germline, F3 male offspring (mF3) displayed anxiety-like behavior in the androgenized and the obese lineages. Using a targeted approach to search for molecular targets within the amygdala, we identified five differentially expressed genes involved in anxiety-like behavior in F3 females in the androgenized lineage and eight genes in the obese lineage. In mF3 male offspring, three genes were dysregulated in the obese lineage but none in the androgenized lineage. Finally, we performed in vitro fertilization (IVF) using a PCOS mouse model of continuous androgen exposure. We showed that the IVF generated F1 and F2 offspring in the female germline did not develop anxiety-like behavior, while the F2 male offspring (mF2) in the male germline did. Our findings provide evidence that elevated maternal androgens in PCOS and maternal obesity may underlie the risk of a transgenerational transmission of anxiety disorders in children of women with PCOS.
Dual RNA 3’-end processing of H2A.X messenger RNA maintains DNA damage repair throughout the cell cycle
Authors: Griesbach Esther , Schlackow Margarita , Marzluff William F. , Proudfoot Nick J. 
Published: January 2021

AbstractPhosphorylated H2A.X is a critical chromatin marker of DNA damage repair (DDR) in higher eukaryotes. However, H2A.X gene expression remains relatively uncharacterised. Replication-dependent (RD) histone genes generate poly(A)- mRNA encoding new histones to package DNA during replication. In contrast, replication-independent (RI) histone genes synthesise poly(A)+ mRNA throughout the cell cycle, translated into histone variants that confer specific epigenetic patterns on chromatin. Remarkably H2AFX, encoding H2A.X, is a hybrid histone gene, generating both poly(A)+ and poly(A)- mRNA isoforms. Here we report that the selective removal of either mRNA isoform reveals different effects in different cell types. In some cells, RD H2A.X poly(A)- mRNA generates sufficient histone for deposition onto DDR associated chromatin. In contrast, cells making predominantly poly(A)+ mRNA require this isoform for de novo H2A.X synthesis, required for efficient DDR. This highlights the importance of differential H2A.X mRNA 3’-end processing in the maintenance of effective DDR.
Regulatory protein HilD stimulates Salmonella Typhimurium invasiveness by promoting smooth swimming via the methyl-accepting chemotaxis protein McpC
Authors: Cooper Kendal G. , Chong Audrey , Kari Laszlo , Jeffrey Brendan , Starr Tregei , Martens Craig , McClurg Molly , Posada Victoria R. , Laughlin Richard C. , Whitfield-Cargile Canaan , Garry Adams L. , Bryan Laura K. , Little Sara V. , Krath Mary , Lawhon Sara D. , Steele-Mortimer Olivia 
Published: January 2021

AbstractIn the enteric pathogen Salmonella enterica serovar Typhimurium, invasion and motility are coordinated by the master regulator HilD, which induces expression of the type III secretion system 1 (T3SS1) and motility genes. Methyl-accepting chemotaxis proteins (MCPs) detect specific ligands and control the direction of the flagellar motor, promoting tumbling and changes in direction (if a repellent is detected) or smooth swimming (in the presence of an attractant). Here, we show that HilD induces smooth swimming by upregulating an uncharacterized MCP (McpC), and this is important for invasion of epithelial cells. Remarkably, in vitro assays show that McpC can suppress tumbling and increase smooth swimming in the absence of exogenous ligands. Expression of mcpC is repressed by the universal regulator H-NS, which can be displaced by HilD. Our results highlight the importance of smooth swimming for Salmonella Typhimurium invasiveness and indicate that McpC can act via a ligand-independent mechanism when incorporated into the chemotactic receptor array.
Non-invasive suppression of essential tremor via phase-locked disruption of its temporal coherence
Authors: Schreglmann Sebastian R. , Wang David , Peach Robert L. , Li Junheng , Zhang Xu , Latorre Anna , Rhodes Edward , Panella Emanuele , Cassara Antonino M. , Boyden Edward S. , Barahona Mauricio , Santaniello Sabato , Rothwell John , Bhatia Kailash P. , Grossman Nir 
Published: January 2021

AbstractAberrant neural oscillations hallmark numerous brain disorders. Here, we first report a method to track the phase of neural oscillations in real-time via endpoint-corrected Hilbert transform (ecHT) that mitigates the characteristic Gibbs distortion. We then used ecHT to show that the aberrant neural oscillation that hallmarks essential tremor (ET) syndrome, the most common adult movement disorder, can be transiently suppressed via transcranial electrical stimulation of the cerebellum phase-locked to the tremor. The tremor suppression is sustained shortly after the end of the stimulation and can be phenomenologically predicted. Finally, we use feature-based statistical-learning and neurophysiological-modelling to show that the suppression of ET is mechanistically attributed to a disruption of the temporal coherence of the aberrant oscillations in the olivocerebellar loop, thus establishing its causal role. The suppression of aberrant neural oscillation via phase-locked driven disruption of temporal coherence may in the future represent a powerful neuromodulatory strategy to treat brain disorders.
Single cell transcriptomic analysis of human pluripotent stem cell chondrogenesis
Authors: Wu Chia-Lung , Dicks Amanda , Steward Nancy , Tang Ruhang , Katz Dakota B. , Choi Yun-Rak , Guilak Farshid 
Published: January 2021

AbstractThe therapeutic application of human induced pluripotent stem cells (hiPSCs) for cartilage regeneration is largely hindered by the low yield of chondrocytes accompanied by unpredictable and heterogeneous off-target differentiation of cells during chondrogenesis. Here, we combine bulk RNA sequencing, single cell RNA sequencing, and bioinformatic analyses, including weighted gene co-expression analysis (WGCNA), to investigate the gene regulatory networks regulating hiPSC differentiation under chondrogenic conditions. We identify specific WNTs and MITF as hub genes governing the generation of off-target differentiation into neural cells and melanocytes during hiPSC chondrogenesis. With heterocellular signaling models, we further show that WNT signaling produced by off-target cells is responsible for inducing chondrocyte hypertrophy. By targeting WNTs and MITF, we eliminate these cell lineages, significantly enhancing the yield and homogeneity of hiPSC-derived chondrocytes. Collectively, our findings identify the trajectories and molecular mechanisms governing cell fate decision in hiPSC chondrogenesis, as well as dynamic transcriptome profiles orchestrating chondrocyte proliferation and differentiation.