bluuebunny/crossref_metadata_2025_split huggingface dataset:
m6A-Related lncRNAs Are Potential Biomarkers for the Prognosis of Metastatic Skin Cutaneous Melanoma
Authors: Huang Suyang , Lyu Shanshan , Gao Zhe , Zha Weifeng , Wang Ping , Shan Yunyun , He Jianzhong , Li Yang 
Published: May 2021

Background: The incidence of skin cutaneous melanoma (SKCM) has risen more rapidly than any other solid tumor in the past few decades. The median survival for metastatic melanoma is only six to nine months and the 5°years survival rate of patients with conventional therapy is less than 5%. Our aim was to reveal the potential molecular mechanism in m6A modification of lncRNA and provide candidate prognostic biomarkers for metastatic SKCM.Methods: lncRNAs expression level was obtained by re-annotation in TCGA and CCLE datasets. m6A-related lncRNAs were selected though correlation analysis. Univariate cox regression analysis was used to screen out independent prognostic factors. LASSO Cox regression was performed to construct an m6A-related lncRNA model (m6A-LncM). Univariate survival analysis and ROC curve were used to assess the prognostic efficacy of this model and candidate lncRNAs. Enrichment analysis was used to explore the candidate genes’ functions.Results: We obtained 1,086 common m6A-related lncRNAs after Pearson correlation analysis in both two datasets. 130 out of the 1,086 lncRNAs are independent prognostic factors. 24 crucial lncRNAs were filtered after LASSO Cox regression analysis. All the m6A-LncM and the 24 lncRNAs were related to overall survival. Stratified survival analysis of m6A-LncM showed that the model retains its prognostic efficacy in recurrence, radiation therapy and other subgroups. Enrichment analysis also found that these lncRNAs were immune associated.Conclusion: Here, we obtained 24 crucial lncRNAs that may be potential biomarkers to predict survival of metastatic SKCM and may provide a new insight to improve the prognosis of it.
New Prediction Models of Functional Outcome in Acute Intracerebral Hemorrhage: The dICH Score and uICH Score
Authors: Yang Wen-Song , Shen Yi-Qing , Wei Xiao , Zhao Li-Bo , Liu Qing-Jun , Xie Xiong-Fei , Zhang Zhi-Wei , Deng Lan , Lv Xin-Ni , Zhang Shu-Qiang , Li Xin-Hui , Li Qi , Xie Peng 
Published: May 2021

Objectives: The original intracerebral hemorrhage (oICH) score is the severity score most commonly used in clinical intracerebral hemorrhage (ICH) research but may be influenced by hematoma expansion or intraventricular hemorrhage (IVH) growth in acute ICH. Here, we aimed to develop new clinical scores to improve the prediction of functional outcomes in patients with ICH.Methods: Patients admitted to the First Affiliated Hospital of Chongqing Medical University with primary ICH were prospectively enrolled in this study. Hematoma volume was measured using a semiautomated, computer-assisted technique. The dynamic ICH (dICH) score was developed by incorporating hematoma expansion and IVH growth into the oICH score. The ultra-early ICH (uICH) score was developed by adding the independent non-contrast CT markers to the oICH score. Receiver operating characteristic curve analysis was used to compare performance among the oICH score, dICH score, and uICH score.Results: There were 310 patients in this study which included 72 patients (23.2%) with hematoma expansion and 58 patients (18.7%) with IVH growth. Of 31 patients with two or more non-contrast computed tomography markers, 61.3% died, and 96.8% had poor outcomes at 90 days. After adjustment for potential confounding variables, we found that age, baseline Glasgow Coma Scale score, presence of IVH on initial CT, baseline ICH volume, infratentorial hemorrhage, hematoma expansion, IVH growth, blend sign, black hole sign, and island sign could independently predict poor outcomes in multivariate analysis. In comparison with the oICH score, the dICH score and uICH score exhibited better performance in the prediction of poor functional outcomes.Conclusions: The dICH score and uICH score were useful clinical assessment tools that could be used for risk stratification concerning functional outcomes and provide guidance in clinical decision-making in acute ICH.
Participation of Acoustic and Electric Hearing in Perceiving Musical Sounds
Authors: Duret Sonia , Bigand Emmanuel , Guigou Caroline , Marty Nicolas , Lalitte Philippe , Bozorg Grayeli Alexis 
Published: May 2021

Introduction: The objective of our study was to evaluate musical perception and its relation to the quality of life in patients with bimodal binaural auditory stimulation.Materials and Methods: Nineteen adult patients with a cochlear implant (CI) for minimum 6 months, and moderate to severe contralateral hearing loss with a hearing aid (HA), and 21 normal hearing adults were included in this prospective, cross-sectional study. Pure-tone and speech audiometry, musical test evaluating sound perception characteristics and musical listening abilities, Munich questionnaire for musical habits, and the APHAB questionnaire were recoded. Performance in musical perception test with HA, CI, and HA + CI, and potential correlations between music test, audiometry and questionnaires were investigated.Results: Bimodal stimulation improved musical perception in several features (sound brightness, roughness, and clarity) in comparison to unimodal hearing, but CI did not add to HA performances in texture, polyphony or musical emotion and even appeared to interfere negatively in pitch perception with HA. Musical perception performances (sound clarity, instrument recognition) appeared to be correlated to hearing-related quality of life (APHAB RV and EC subdomains) but not with speech performances suggesting that the exploration of musical perception complements speech understanding evaluation to better describe every-day life hearing handicap.Conclusion: Testing musical sound perception provides important information on hearing performances as a complement to speech audiometry and appears to be related to hearing-related quality of life.
A Preliminary Experience of Implementing Deep-Learning Based Auto-Segmentation in Head and Neck Cancer: A Study on Real-World Clinical Cases
Authors: Zhong Yang , Yang Yanju , Fang Yingtao , Wang Jiazhou , Hu Weigang 
Published: May 2021

PurposeWhile artificial intelligence has shown great promise in organs-at-risk (OARs) auto segmentation for head and neck cancer (HNC) radiotherapy, to reach the level of clinical acceptance of this technology in real-world routine practice is still a challenge. The purpose of this study was to validate a U-net-based full convolutional neural network (CNN) for the automatic delineation of OARs of HNC, focusing on clinical implementation and evaluation.MethodsIn the first phase, the CNN was trained on 364 clinical HNC patients’ CT images with annotated contouring from routine clinical cases by different oncologists. The automated delineation accuracy was quantified using the Dice similarity coefficient (DSC) and 95% Hausdorff distance (HD). To assess efficiency, the time required to edit the auto-contours to a clinically acceptable standard was evaluated by a questionnaire. For subjective evaluation, expert oncologists (more than 10 years’ experience) were randomly presented with automated delineations or manual contours of 15 OARs for 30 patient cases. In the second phase, the network was retrained with an additional 300 patients, which were generated by pre-trained CNN and edited by oncologists until to meet clinical acceptance.ResultsBased on DSC, the CNN performed best for the spinal cord, brainstem, temporal lobe, eyes, optic nerve, parotid glands and larynx (DSC &gt;0.7). Higher conformity for the OARs delineation was achieved by retraining our architecture, largest DSC improvement on oral cavity (0.53 to 0.93). Compared with the manual delineation time, after using auto-contouring, this duration was significantly shortened from hours to minutes. In the subjective evaluation, two observes showed an apparent inclination on automatic OARs contouring, even for relatively low DSC values. Most of the automated OARs segmentation can reach the clinical acceptance level compared to manual delineations.ConclusionsAfter retraining, the CNN developed for OARs automated delineation in HNC was proved to be more robust, efficiency and consistency in clinical practice. Deep learning-based auto-segmentation shows great potential to alleviate the labor-intensive contouring of OAR for radiotherapy treatment planning.
Increased AOC1 Expression Promotes Cancer Progression in Colorectal Cancer
Authors: Liu Fangyuan , Ou Weijun , Tang Wenbo , Huang Zhenyu , Zhu Zhehui , Ding Wenjun , Fu Jihong , Zhu Yilian , Liu Chenying , Xu Weimin , Du Peng 
Published: May 2021

BackgroundAmine oxidase copper containing 1 (AOC1) is a gene whose biological function in colorectal cancer (CRC) has not been elucidated. Therefore, the purpose of this study was to investigate the clinical significance of AOC1 expression in CRC and its biological function in CRC cell lines.Materials and MethodsAOC1 expression levels were examined in paired CRC and peritumoral tissues, and distant liver metastatic tissues were examined using quantitative real-time PCR, western blotting, and immunohistochemistry staining. The log-rank test and Cox regression model were used to analyze the relationship between AOC1 expression and prognosis. Proliferation assays (Cell Counting Kit‐8 and colony formation assays), migration assays (Transwell and wound healing assays) and xenograft tumor formation in nude mice were performed to assess the biological role of AOC1 in CRC cells.ResultsAOC1 expression significantly increased in human CRC tissues, especially in liver metastases, and was associated with a worse prognosis. In addition, AOC1 had higher expression in tumor organoids than in normal organoids, suggesting that it was highly expressed in the tumor epithelium. Functional analysis demonstrated that AOC1 knockdown inhibited the proliferation and migration of CRC cells by inducing EMT in vitro. Xenograft tumor formation in nude mice showed that knockdown of AOC1 inhibited the tumor xenografts growth in vivo.ConclusionHigh expression of AOC1 was significantly associated with worse clinical outcomes, was an independent risk factor for poor prognosis, and promoted aggressive CRC cell phenotypes. AOC1 is expected to become a novel biomarker for predicting the prognosis of patients with CRC and an effective therapeutic target in clinical practice.
Quercitrin Attenuates Acetaminophen-Induced Acute Liver Injury by Maintaining Mitochondrial Complex I Activity
Authors: Xiong Weichen , Yuan Zixin , Wang Tianshun , Wu Songtao , Xiong Yiyi , Yao Yunfeng , Yang Yanfang , Wu Hezhen 
Published: May 2021

The flavonoid quercitrin has a strong antioxidant property. It is also reported to have a protective effect on the liver. However, the mechanism by which it exerts a protective effect on the liver is not fully understood. The objective of this article is to confirm the protective effect of quercitrin extracted from Albiziae flos on acetaminophen (APAP)-induced liver injury and to explain its mechanism. In the in vivo study, quercitrin was administered orally to BALB/c mice at a dose of 50, 100, and 200 mg/kg for seven consecutive days. APAP (300 mg/kg) was injected intraperitoneally after a last dose of quercitrin was administered. Determination of alanine aminotransferase (ALT), aspartate aminotransferase (AST), lactate dehydrogenase (LDH), interleukin 6 (IL-6), tumor necrosis factor α (TNF-α), reactive oxygen species (ROS), superoxide dismutase (SOD), glutathione (GSH), glutathione peroxidase (GSH-Px), catalase (CAT), and malondialdehyde (MDA) levels showed that quercitrin effectively attenuated APAP-induced acute liver injury in mice. Results of the in vitro study showed that quercitrin reduced the levels of ROS, protected mitochondria from damage, and restored the activity of mitochondrial complex I in APAP-treated L-02 cells. The addition of rotenone which is an inhibitor of complex I blocked the protective effect of quercitrin. The expression of mitochondrial complex I was also maintained by quercitrin. Our results suggest that quercitrin can maintain the level of mitochondrial complex I in injured cells and restore its activity, which reduces the production of ROS, protects the mitochondria from oxidative stress, and has a protective effect on the liver.
Perspectives on a Way Forward to Implementation of Precision Medicine in Patients With Diabetic Kidney Disease; Results of a Stakeholder Consensus-Building Meeting
Authors: Bakker Elisabeth , Mol Peter G. M. , Nabais João , Vetter Thorsten , Kretzler Matthias , Nolan John J. , Mayer Gert , Sundgren Anna K. , Heerspink Hiddo J. L. , Schiel Anja , de Vries Sieta T. , Gomez Maria F. , Schulze Friedrich , de Zeeuw Dick , Pena Michelle J. ,   for the BEAt-DKD Consortium
Published: May 2021

Aim: This study aimed to identify from different stakeholders the benefits and obstacles of implementing precision medicine in diabetic kidney disease (DKD) and to build consensus about a way forward in order to treat, prevent, or even reverse this disease.Methods: As part of an ongoing effort of moving implementation of precision medicine in DKD forward, a two-day consensus-building meeting was organized with different stakeholders involved in drug development and patient care in DKD, including patients, patient representatives, pharmaceutical industry, regulatory agencies representatives, health technology assessors, healthcare professionals, basic scientists, and clinical academic researchers. The meeting consisted of plenary presentations and discussions, and small group break-out sessions. Discussion topics were based on a symposium, focus groups and literature search. Benefits, obstacles and potential solutions toward implementing precision medicine were discussed. Results from the break-out sessions were presented in plenary and formed the basis of a broad consensus discussion to reach final conclusions. Throughout the meeting, participants answered several statement and open-ended questions on their mobile device, using a real-time online survey tool. Answers to the statement questions were analyzed descriptively. Results of the open-ended survey questions, the break-out sessions and the consensus discussion were analyzed qualitatively.Results and conclusion: Seventy-one participants from 26 countries attended the consensus-building meeting in Amsterdam, April 2019. During the opening plenary on the first day, the participants agreed with the statement that precision medicine is the way forward in DKD (n = 57, median 90, IQR [75–100]). Lack of efficient tools for implementation in practice and generating robust data were identified as significant obstacles. The identified benefits, e.g., improvement of the benefit-risk ratio of treatment, offer substantive incentives to find solutions for the identified obstacles. Earlier and increased multi-stakeholder collaboration and specific training may provide solutions to alter clinical and regulatory guidelines that lie at the basis of both obstacles and solutions. At the end of the second day, the opinion of the participants toward precision medicine in DKD was somewhat more nuanced (n = 45, median 83, IQR [70–92]) and they concluded that precision medicine is an important way forward in improving the treatment of patients with DKD.
Adverse Reactions to Drugs of Special Interest in a Pediatric Oncohematology Service
Authors: Amaro-Hosey Kristopher , Danés Immaculada , Vendrell Lourdes , Alonso Laura , Renedo Berta , Gros Luis , Vidal Xavier , Cereza Gloria , Agustí Antònia 
Published: May 2021

Introduction: Drugs used in oncological diseases are frequently related to adverse drug reactions (ADR). Few studies have analyzed the toxicity of cancer treatments in children in real practice.Methods: An observational, longitudinal and prospective study has been carried out in an Oncohematology Service of a tertiary hospital. During 2017, patients exposed to one or more drugs of a previously agreed list were identified and followed-up for at least 6 months each. Characteristics of ADR, incidence, causality and possible preventability, have been evaluated.Results: 72 patients have been treated with at least one study drug, and 159 ADR episodes involving at least one of these drugs have been identified, with a total of 293 ADR. Most episodes required hospital admission (35.2%) or happened during the hospital stay (33%), and 91.2% were severe. Blood disorders were the most frequent ADR (96; 32.8%), related to thioguanine (42) and pegaspargase (39) mainly, followed by infections (86; 29.4%) related to thioguanine (32), pegaspargase (27), Erwinia asparaginase (14) and rituximab (13). Two ADR were unknown. Most ADR were dose-dependent or expectable (&gt;90%). The global incidence of ADR was 3.1/100 days at risk (95% CI 2.7–3.5), with 3.5 ADR/100 days at risk with pegaspargase (95% CI 2.9–4.2), 1.2/100 days at risk with rituximab (95% CI 0.8–1.8) and 11.6/100 days at risk with thioguanine (95% CI 9.4–14.2). Controversial additional measures of prevention, other than those already used, were identified.Conclusion: ADR are frequent in pediatric oncohematological patients, mainly blood disorders and infectious diseases. Findings regarding incidence and preventability may be useful to compare data between different centers and to evaluate new possibilities for action or prevention.
Multidirectional Effects of Tormentil Extract on Hemostasis in Experimental Diabetes
Authors: Marcinczyk Natalia , Gołaszewska Agata , Gromotowicz-Poplawska Anna , Misztal Tomasz , Strawa Jakub , Tomczyk Michał , Kasacka Irena , Chabielska Ewa 
Published: May 2021

In our previous study, we showed that ellagitannin- and procyanidin-rich tormentil extract (TE) decreased experimental arterial thrombosis in normoglycemic rats through platelet inhibition. TE also slightly increased coagulation and attenuated fibrinolysis; however, these effects did not nullify the antithrombotic effect of TE. The present study aimed to assess whether TE exerts antithrombotic activity in streptozotocin (STZ)-induced diabetes, which is characterized by pre-existing increased coagulation and impaired fibrinolysis, in vivo and ex vivo thrombosis assays. TE (100, 200, or 400 mg/kg, p. o.) was administered for 14 days to STZ-induced diabetic rats and mice. TE at 100 mg/kg dose decreased the thrombus area in the mice model of laser-induced thrombosis through its potent antiplatelet effect. However, TE at 200 mg/kg dose increased thrombus weight in electrically induced arterial thrombosis in rats. The prothrombotic effect could be due to increased coagulation and attenuated fibrinolysis. TE at 400 mg/kg dose also improved vascular functions, which was mainly reflected as an increase in the arterial blood flow, bleeding time prolongation, and thickening of the arterial wall. However, TE at 400 mg/kg dose did not exert antithrombotic effect. Summarizing, the present results show that TE may exert multidirectional effects on hemostasis in STZ-induced diabetic rats and mice. TE inhibited platelet activity and improved endothelial functions, but it also showed unfavorable effects by increasing the activity of the coagulation system and by inhibiting fibrinolysis. These contrasting effects could be the reason for model-specific influence of TE on the thrombotic process in STZ-induced diabetes.
Loneliness and Healthcare Use in Older Adults: Evidence From a Nationally Representative Cohort in Northern Ireland—A Cross-Sectional Replication Study
Authors: Burns Annette , Leavey Gerard , O'Sullivan Roger 
Published: May 2021

Background: Few have explored associations between loneliness and healthcare use independent of health and health behaviors. Recent indication of gender effects also requires validation across health service and cultural settings. We investigated the associations among loneliness, health and healthcare use (HCU) in older adults including stratification to investigate whether associations differed by gender.Methods: Secondary analysis of a nationally representative sample of 8,309 community-dwelling adults aged 50 and over from the Northern Ireland Cohort for the Longitudinal Study of Aging. Primary outcomes were: self-reported General Practice (GP) and emergency department (ED) visits in past year. Negative binomial and logistic regression analysis were used to investigate associations between loneliness and HCU, later adjusting for potential confounders (health and health behaviors).Results: Loneliness was consistently positively associated with both GP and ED visits (with IRRs ranging from 1.10 to 1.49 for GP visits, 1.16 to 1.98 for ED visits and ORs ranging from 1.13 to 1.51 for reporting at least one ED visit). With addition of health and health behaviors, all associations between loneliness and HCU became non-significant, excepting a small independent association between UCLA score and GP visits [IRR 1.03 (95% CI 1.01–1.05)]. Stratification of models revealed no gender effects.Conclusion: All but one association between loneliness and HCU became non-significant when health and health behaviors were included. The remaining association was small but implications remain for health service resources at population level. No gender effects were present in contrast to recent findings in the Republic of Ireland. Further studies on gender, loneliness and healthcare use needed.
Barriers to Exiting and Factors Contributing to the Cycle of Enter/Exit/Re-Entering Commericial Sex Work
Authors: Dinse Ling , Rice Karen 
Published: May 2021

Commercial sex work is a high-risk job and individuals who desire to leave the sex industry find the process of exiting complex and full of obstacles. This qualitative study identified the barriers to exiting and factors contributing to the cycle of enter/exit/re-entering commercial sex work. This study found barriers in the individual, social, relational, and structural domains. The results validated the use of the polymorphous paradigm to comprehend the challenges of exit and factors that reinforce the cycle of enter/exit/re-entering the industry. The expanded knowledge from this study yielded insight for service providers to develop appropriate programs and services. In addition, the study results challenged the faith community’s response to individuals engaged in the commercial sex industry.
ТРАНСФОРМАЦІЯ ПЛАНУВАННЯ СОЦІАЛЬНО-ЕКОНОМІЧНОГО РОЗВИТКУ ТА ЙОГО РОЛІ В ДЕРЖАВНОМУ УПРАВЛІННІ
Authors: Bubliy  M.  , Drobiazko  L. 
Published: May 2021

Досліджено трансформацію планування від директивного до стратегічного та його роль у державному управлінні соціально-економічним розвитком. З’ясовано, що саме система стратегічного планування і державне управління на її основі являють собою найбільш сучасну модель управління, яка є основною сполучною ланкою, необхідною передумовою до подальшої модернізації діяльності органів влади і напрямом подальшої оптимізації процесів. Стратегічне управління в найбільш комплексному вигляді реалізує ту ж логіку системного походу, на яку спирається розвиток державного управління в рамках сучасних форм NPM, “ефективного управління” і “держави розвитку”.
Доведено, що розвиток соціально-економічних систем у сучасних умовах вимагає застосування нових технологій управління і прийняття рішень, що забезпечують більш широке врахування суспільних вимог, необхідності вирішення проблем не тільки економіки, а й соціальної сфери. Методологічно обґрунтовано обов’язковість використання системи стратегічного планування в діяльності органів управління соціально-економічним розвитком.