bluuebunny/crossref_metadata_2025_split huggingface dataset:
Modulation of the Acetylcholine Receptor Clustering Pathway Improves Neuromuscular Junction Structure and Muscle Strength in a Mouse Model of Congenital Myasthenic Syndrome
Authors: Spendiff Sally , Howarth Rachel , McMacken Grace , Davey Tracey , Quinlan Kaitlyn , O'Connor Emily , Slater Clarke , Hettwer Stefan , Mäder Armin , Roos Andreas , Horvath Rita , Lochmüller Hanns 
Published: January 2021

Introduction: Congenital myasthenic syndromes (CMS) are a diverse group of inherited neuromuscular disorders characterized by a failure of synaptic transmission at the neuromuscular junction (NMJ). CMS often present early with fatigable weakness and can be fatal through respiratory complications. The AGRN gene is one of over 30 genes known to harbor mutations causative for CMS. In this study, we aimed to determine if a compound (NT1654), developed to stimulate the acetylcholine receptor (AChR) clustering pathway, would benefit a mouse model of CMS caused by a loss-of-function mutation in Agrn (Agrnnmf380 mouse).Methods:Agrnnmf380 mice received an injection of either NT1654 or vehicle compound daily, with wild-type litter mates used for comparison. Animals were weighed daily and underwent grip strength assessments. After 30 days of treatment animals were sacrificed, and muscles collected. Investigations into NMJ and muscle morphology were performed on collected tissue.Results: While minimal improvements in NMJ ultrastructure were observed with electron microscopy, gross NMJ structure analysis using fluorescent labelling and confocal microscopy revealed extensive postsynaptic improvements in Agrnnmf380 mice with NT1654 administration, with variables frequently returning to wild type levels. An improvement in muscle weight and myofiber characteristics helped increase forelimb grip strength and body weight.Conclusions: We conclude that NT1654 restores NMJ postsynaptic structure and improves muscle strength through normalization of muscle fiber composition and the prevention of atrophy. We hypothesize this occurs through the AChR clustering pathway in Agrnnmf380 mice. Future studies should investigate if this may represent a viable treatment option for patients with CMS, especially those with mutations in proteins of the AChR clustering pathway.
RETRACTED: FNDC1 Promotes the Invasiveness of Gastric Cancer via Wnt/β-Catenin Signaling Pathway and Correlates With Peritoneal Metastasis and Prognosis
Authors: Jiang Tao , Gao Wenyu , Lin Shengjie , Chen Hao , Du Bin , Liu Qing , Lin Xiaoyan , Chen Qiang 
Published: January 2021

BackgroundGastric cancer (GC) has a high morbidity and mortality rate, with peritoneal metastasis (PM) identified as the main site of metastasis. Our previous study found that FNDC1 has a higher frequency of mutations in patients with PM by high-throughput sequencing assay, suggesting that it may be associated with GC invasion and PM, however the specific mechanism remains unclear.MethodsFirst, the correlation between FNDC1 and PM and prognosis of GC was clarified by bioinformatics and clinicopathological analysis. Next, the effect of FNDC1 expression on the invasion and metastasis ability of GC was investigated in vivo and in vitro. Finally, the signaling pathways involved in the regulation of FNDC1 were explored.ResultsFNDC1 was highly expressed in GC and was associated with PM and poor prognosis. FNDC1 was also associated with epithelial-mesenchymal transition (EMT) in GC cells. Through in vivo and in vitro experiments, it was clarified that knockdown of FNDC1 could inhibit the proliferation, invasion, and migration of GC cells. In addition, it was elucidated that FNDC1 promotes EMT through the Wnt/β-catenin signaling pathway.ConclusionFNDC1 may be associated with the invasion of GC and PM after surgery. FNDC1 was highly expressed in GC tissues and cell lines, while significantly associated with poor DFS and OS in GC patients. Both univariate and multivariate analyses suggested that the expression of FNDC1 was an independent factor for GC. Knockdown of FNDC1 also significantly inhibited the proliferation, migration, and activity of GC cells. FNDC1 may promote EMT in GC cells through the regulation of Wnt/β-catenin signaling pathway. FNDC1 has the potential to be used as a predictor of PM and may also be studied in depth as a therapeutic target for GC, which has potential clinical utility and is worthy of further validation.
Solitary Metastasis in the Mediastinal Lymph Node After Radical Nephrectomy for Clear Cell Renal Cell Carcinoma: A Case Report and Literature Review
Authors: Lin Hang , Zhang Heng , Cheng Yuanda , Zhang Chunfang 
Published: January 2021

BackgroundRenal cell carcinoma can metastasize to virtually any anatomical site throughout the body, especially the lung, bone, lymph nodes, liver, and brain. However, it is extremely rare for renal cell carcinoma to metastasize solely to the mediastinal lymph node more than 15 years after radical nephrectomy.Case PresentationThe case we present here is that of a 50-year-old Chinese male with an isolated posterior mediastinal lymph node metastasis of clear cell renal cell carcinoma 16 years after radical nephrectomy. However, based on imaging examination, the mass was clinically misdiagnosed as Castleman’s disease before operation. Following surgical excision of the mass, it was finally judged to be a metastasis from clear cell renal cell carcinoma according to the patient’s medical history and immunohistochemical findings. Currently, there is no clinical or radiological finding the recurrence of metastasis after 10 months of follow-up.ConclusionWe report a case of solitary metastasis in the posterior mediastinal lymph node 16 years after radical nephrectomy for clear cell renal cell carcinoma. Given the long disease-free interval between primary renal cell carcinoma to isolated mediastinal lymph node metastasis, it is important to conduct a lifelong regular follow-up, including thoracic computed tomography. In addition, surgical resection remains the best method of treatment for mediastinal lymph node metastases from clear cell renal cell carcinoma if the metastatic lesion is limited.
Kawasaki Disease and Allergic Diseases
Authors: Huang Po-Yu , Huang Ying-Hsien , Guo Mindy Ming-Huey , Chang Ling-Sai , Kuo Ho-Chang 
Published: January 2021

Background: Kawasaki disease (KD) is an inflammatory disorder with an unknown etiology. It is the leading cause of acquired heart disease, which leads to coronary vasculitis among children. Studies of frequent manifestation of allergic diseases in children with KD have been the subject of mounting clinical interest. However, evidence supporting the association between KD and allergies has yet to be systematically reviewed.Methods: In this article, we reviewed current literature regarding the association between KD and allergic diseases. References for this review were identified through searches of PubMed, Cochrane, and Embase through the end of August 2020.Results: The results of the analyses of immune repertoire, clinical, and epidemiological studies have indicated some of the characteristics of infectious disease for KD. Although some allergic disorders, such as asthma, may be exacerbated by viral infections, allergies are typically caused by an allergen that triggers an immune response, with the potential involvement of type 2 inflammation and immune disturbances leading to tissue remodeling in genetically susceptible hosts. The effect of intravenous immunoglobulin is multi-faceted and results in a decrease in activating Fc gamma receptor IIA and an increase in anti-inflammatory eosinophils. The findings from this review demonstrate that children who have suffered from KD are more likely to have allergic rhinitis than the general population and their siblings, a condition that lasts until the age of 17. When followed up as teenagers and adults, children with KD are more likely to develop urticaria.Conclusions: This review supports that allergic diseases, such as allergic rhinitis, have been demonstrated to increase following KD. Therefore, the importance of allergic diseases in patients with KD should be emphasized in long-term care. Interventions that include strategies for managing allergies in children with KD would be beneficial.
No Evidence That Hyperpnea-Based Respiratory Muscle Training Affects Indexes of Cardiovascular Health in Young Healthy Adults
Authors: Beltrami Fernando G. , Mzee David , Spengler Christina M. 
Published: January 2021

IntroductionThe chronic effects of respiratory muscle training (RMT) on the cardiovascular system remain unclear. This investigation tested to which degree a single sessions of RMT with or without added vibration, which could enhance peripheral blood flow and vascular response, or a 4-week RMT program could result in changes in pulse wave velocity (PWV), blood pressure (systolic, SBP; diastolic, DBP) and other markers of cardiovascular health.MethodsSixteen young and healthy participants (8 m/8f) performed 15 min of either continuous normocapnic hyperpnea (RMET), sprint-interval-type hyperpnea (RMSIT) or a control session (quiet sitting). Sessions were performed once with and once without passive vibration of the lower limbs. To assess training-induced adaptations, thirty-four young and healthy participants (17 m/17f) were measured before and after 4 weeks (three weekly sessions) of RMET (n = 13, 30-min sessions of normocapnic hyperpnea), RMSIT [n = 11, 6 × 1 min (1 min break) normocapnic hyperpnea with added resistance] or placebo (n = 10).ResultsSBP was elevated from baseline at 5 min after each RMT session, but returned to baseline levels after 15 min, whereas DBP was unchanged from baseline following RMT. Carotid-femoral PWV (PWVCF) was elevated at 5 and 15 min after RMT compared to baseline (main effect of time, P = 0.001), whereas no changes were seen for carotid-radial PWV (PWVCR) or the PWVCF/PWVCR ratio. Vibration had no effects in any of the interventions. Following the 4-week training period, no differences from the placebo group were seen for SBP (P = 0.686), DBP (P = 0.233), PWVCF (P = 0.844), PWVCR (P = 0.815) or the PWVCF/PWVCR ratio (P = 0.389).Discussion/ConclusionAlthough 15 min of RMT sessions elicited transient increases in PWVCF and SBP, no changes were detected following 4 weeks of either RMET or RMSIT. Adding passive vibration of the lower limbs during RMT sessions did not provide additional value to the session with regards to vascular responses.
“We Copy to Join in, to Not Be Lonely”: Adolescents in Special Education Reflect on Using Dramatic Imitation in Group Dramatherapy to Enhance Relational Connection and Belonging
Authors: Musicka-Williams Amanda 
Published: January 2021

This paper focuses on doctoral research which explored relationships and interpersonal learning through group dramatherapy and creative interviewing with adolescents in special education. A constructivist grounded theory study, positioning adolescents with intellectual/developmental disabilities as experts of their own relational experiences, revealed a tendency to“copy others.”The final grounded theory presented“copying”as a tool which participants consciously employed “to play with,” “learn from,”and“join in with”others. Commonly experiencing social ostracism, participants reflected awareness of their tendency to“copy others”being underpinned by a need to belong. Belonging was therefore expressed as the ultimate therapeutic experience participants wished to have. Participant responses which link dramatic imitation to a self-identified tendency“to copy,”are discussed with regard to how imitation provides an accessible point of dramatic entry from which adolescents in special education begin to explore new ways of being and inter-relating. Recommendations for how dramatherapists might centralize imitative aspects of the dramatic process to achieve therapeutic intent when working alongside adolescents in special education are discussed with specific focus on creating a space of belonging.Note on type: Participant quotes extracted from the data are included throughout this article. In order to highlight participant’s contributions quotes are italicized and presented within speech marks.
Penerapan Sistem Pengendalian Internal Persediaan Bahan Baku Pada PT. Wings Surya
Authors: Yezhica Kumala  , Sugijanto  
Published: January 2021

Pengendalian iternal persediana pada PT.Wings Surya harus dilakukan untuk mempermudah mengontrol aktivitas persediaan yang berjalan sesuai yang diharapkan oleh perusahaan. Pengendalian internal persediaan dilakukan untuk meminimalisir kesalahan dan kecurangan dalam proses persediaan, karena ada pelaksana dilapangan yang sengaja melakukan kecurangan dalam menjalankan tugasnya, namun juga ada pelaksana yang melakukan kesalahan tapi mereka tidak sengaja atau tidak ada niat. Kesalahan tersebut dikarenakan ada suatu prosedur yang tidak dijalankan. Oleh sebab itu dengan adanya pengendalian internal padaa PT.Wings surya adapat menunjang kinerja karyawan dalam menjalankan tugasnya dengan baik.
PENGARUH HARGA, KUALITAS PRODUK, KUALITAS LAYANAN & LOKASI TERHADAP KEPUTUSAN PEMBELIAN LAPIS KUKUS PAHLAWAN
Authors: Rahajeng Irna Sari  , Siti Istikhoroh  
Published: January 2021

Dijelaskan riset menganalisa harga, kualitas produk, kualitas layanan serta lokasi terhadap keputusan pembelian lapis kukus pahlawan. Riset ini memakai pendekatakan kuantitatif deskriptif, telah diperoleh hasil thitung 2,564 serta Sig. sebesar ,012 sehingga ,012 < ,05. kualitas produk 2,055 sig. ,043<,05. kualitas layanan 2,216 serta Sig. ,036. lokasi 2,231 serta Sig. ,028. nilai f-hitung serta diketahui F hitung 5,972 serta sig. ,000 < 0,05. Disimpulkan pengujian secara terpisah serta bersamaan terbukti adanya pengaruh variabel bebas terhadap variabel terikat.
PREDIKSI JUMLAH PENUMPANG KERETA API DENGAN MENGGUNAKAN METODE ARIMA
Authors: Ria Maria letisia , Indrasetianingsih Artanti 
Published: January 2021

Kereta api merupakan sub sektor perhubungan darat yang dianggap penting didalam usaha melayani jasa perhubungan masyarakat. Tujuan dari penelitian ini untuk mendapatkan model ARIMA terbaik dan prediksi jumlah penumpang kereta api untuk satu tahun kedepan. Analisis yang digunakan adalah analisis runtun waktu yaitu model ARIMA. Berdasarkan hasil analisis adalah model ARIMA(1,1,0)(0,1,1)12, dengan nilai MSE =551425, nilai RMSE= 872,9009 dan MAPE 9,8%. Model ARIMA (1,1,0)(0,1,1)12 adalah
Didapat hasil peramalan jumlah penumpang kereta api dari bulan Maret tahun 2015 hingga bulan Februari tahun 2016. Dari hasil peramalan jumlah penumpang yang tertinggi adalah pada bulan Desember tahun 2015 yaitu 27.134 penumpang
Pengaruh Permainan Lego Terhadap Peningkatan Konsentrasi Anak Autis
Authors: Nur Rohimah Tisnawati  
Published: January 2021

Anak autis memiliki hambatan pada perkembangannya, salah satunya keterlambatan dalam aspek kognitif sehingga berdampak pada adanya gangguan konsentrasi. Gangguan konsentrasi memiliki dampak terutama pada proses belajar anak, sehingga perlu adanya pembelajaran yang menyenangkan melalui aktivitas bermain lego. Lego merupakan permainan konstuktif yang terbuat dari plastik dan berwarna warni. Tujuan penelitian ini adalah untuk mengetahui pengaruh bermain lego terhadap peningkatan konsentrasi anak autis. Penelitian ini menggunakan jenis penelitian eksperimen dengan Single Subject Research (SSR), serta menggunakan desain A-B-A. Jenis ukuran pada penelitian ini menggunakan durasi. Teknik pengumpulan data yang digunakan dalam penelitian ini adalah observasi serta dianalisis dengan analisis visual dalam kondisi dan antar kondisi. Hasil penelitian menunjukkan bahwa durasi rata rata konsentrasi anak pada fase baseline (A1) yaitu 172 detik, kemudian pada fase intervensi (B) yaitu 250 detik, dan pada fase pengulangan baseline (A2) yaitu 281 detik. Hal ini menunjukkan bahwa terdapat perbedaan sebelum dan sesudah diberikannya aktivitas bermain lego. Sehingga dapat disimpulkan bahwa hasil penelitian ini menunjukkan adanya pengaruh bermain lego terhadap peningkatan konsentrasi anak autis.
Karakteristik Buah Dan Biji Cabai Rawit (Capsicum frustescens L.) Yang Diberi Pupuk Organik Cair Rebung Bambu Terfermentasi Ragi Tempe
Authors: Buksalwembun Antonius , Andriani Vivin 
Published: January 2021

Tanaman cabai rawit (Capsicum frustescens L.) merupakan salah satu tanaman sayuran yang penting bagi masyarakat sebagai penambah cita rasa dalam masakan sehari-hari. Tujuan penelitian ini adalah untuk mengetahui pengaruh pemberian pupuk organik cair rebung bambu terfermentasi ragi tempe terhadap karakteristik buah dan biji serta hasil panen cabai rawit. Penelitian ini bersifat eksperimen menggunakan RAL dengan 4 perlakuan 0 ml/l, 5 ml/l, 10 ml/l dan 15 ml/l pupuk organik cair rebung bambu terfermentasi ragi tempe 6 kali ulangan. Hasil penelitian ini menunjukkan bahwa produksi buah cabai rawit dengan konsentrasi 10 ml/l menghasilkan produksi jumlah buah terbaik dengan rata-rata 68,17 gram dan dapat mereduksi jumlah biji cabai rawit sebesar 1.584 biji.
Kata Kunci : Cabai rawit (Capsicum frustescens L.); rebung bambu; fermentasi; ragi tempe; produksi.
Pengaruh Ekstrak Air Selada Laut (Ulva Lactuca) Terhadap Berat Badan Pada Tikus Diabetes
Authors: Rinawati Rinawati , Eky Nursia N Lili , Wahyuni Muhsin Sri , Maisyaroh Fitri Siregar Siti 
Published: January 2021

Unhealthy lifestyle is one of the leading causes to Diabetes Mellitus (DM) disorder which is characterized by beyond normal limits of high blood glucose levels. Seawed (Ulva lactuca) is one type of natural material that is potentially to be anti-DM. Unfortunately, it is not familiar yet to the wider community, especially in Aceh. The purpose of this study was to determine the effect of giving Ulva lactuca water extract to the body weight of DM rats. The research is an experimental completely randomized design (CRD). Rats were grouped into 5 treatments with three replications, namely: KN = normal control (only fed and distilled water), Ka = STZ control (rat induced STZ 65 mg), P1 = rat DM + extract dose 200 mg / KgBB, P2 = DM rats + extract dose 300 mg / KgBB, P3 = DM rats + extract dose 400 mg / KgBB. Measurement of body weight (BB) of rats was carried out for 28 days. Data analysis was performed statistically (ANOVA). The results showed that the water extract of Ulva lactuca affected the changes in body weight of DM rats. The treatment groups of P1, P2 and P3 gained weight after being given Ulva lactuca water extract. The highest percentage of weight increase was found in the P2 and P3 treatment groups.

Keywords: Diabetes Mellitus, Weight, Sea Lettuce (Ulva lactuca)
Penguatan Pendidikan Karakter dan Budi Pekerti melalui Metode Story Telling Bagi Guru Pondok Darunnadwah Cikarang - Bekasi
Authors: Widiyarto Sigit , Muthia Mubasyira  , Leni Tiwinyanti  , Lidya Natalia Sartono  , Siti Alifah  , Adhis Pamungkas  
Published: January 2021

The Strengthening Character Education and Character through Story Telling Method with Darunnadwah Islamic Boarding School partners located at Jln. Gatot Subroto No. 55, Karangasih, North Cikarang, Bekasi, West Java is a community service program with the aim of strengthening the character education and character of students in partner locations due to the lack of understanding of the use of story telling methods in teaching and learning activities in schools. The method used is the lecture and discussion method. Pondok teachers are provided with structured material and the provision of several videos containing examples of story telling. This gives a positive influence in the form of new knowledge and skills, storytelling skills that are useful in teaching in the classroom. The results of this activity are, the enthusiasm or learning motivation of trainees should be appreciated and the teachers already have basic knowledge in applying story telling methods in strengthening character education and students' character and some techniques in reading stories in English.
Keywords: Story Telling Method, Character Education, Character,
Method development for the quantitative determination of captopril from Caco-2 cell monolayers by using LC-MS/MS
Authors: Logoyda Liliya , Piponski Marjan , Kovalenko Sergiy , Denefil Olha , Dutchak Olha , Soroka Yuriy , Pidruchna Svitlana , Popovych Dariya , Susla Oleksandr 
Published: January 2021

Aim. Caco-2 cells are a human colon epithelial cancer cell line used as a model of human intestinal absorption of drugs and other compounds. Although compounds were used in the original Caco-2 cells monolayer assays, compounds have been replaced in most laboratories by the use of liquid chromatography-mass spectrometry (LC-MS) and LC-tandem mass spectrometry (LC-MS/MS). Mass spectrometry not only eliminates the need for compounds, but permits the simultaneous measurement of multiple compounds. The measurement of multiple compounds per assay reduces the number of incubations that need to be carried out, thereby increasing the throughput of the experiments. Furthermore, LC-MS and LC-MS-MS add another dimension to Caco-2 assays by facilitating the investigation of the metabolism of compounds by Caco-2 cells. A simple, rapid LC-MS/MS method has been developed for determination of captopril from confluent Caco-2 monolayers and from aqueous solution.
Materials and methods. Chromatography was achieved on Discovery C18, 50 × 2.1 mm, 5 μm column. Samples were chromatographed in a gradient mode (eluent A (acetonitrile – water – formic acid, 5 : 95 : 0.1 v/v), eluent B (acetonitrile – formic acid, 100 : 0.1 v/v)). The initial content of the eluent B is 0%, which increases linearly by 1.0 min to 100% and to 1.01 min returns to the initial 0%. The mobile phase was delivered at a flow rate of 0.4 mL/min into the mass spectrometer ESI chamber. The sample volume was 5 μl.
Results. Under these conditions, captopril was eluted at 1.42 min. A linear response function was established at 2 – 200 ng/mL. The regression equation for the analysis was y =0.0187x+0.000248 with coefficient of correction (r2) = 0.9993. According to the Caco-2 test results, captopril showed low permeability. It should be noted that the recovery value is 103.20%. The within-run coefficients of variation ranged between 0.321% and 0.541%. The within-run percentages of nominal concentrations ranged between 99.13% and 101.12%. The between-run coefficients of variation ranged between 0.314% and 0.663%. The between-run percentages of nominal concentrations ranged between 99.17% and 101.03%.The assay values on both the occasions (intra- and inter-day) were found to be within the accepted limits.
Conclusion. From results of analysis, it can be concluded that developed method is simple and rapid for determination of captopril from confluent Caco-2 monolayers and from aqueous solution. Acquired results demonstrate that proposed strategy can be effortlessly and advantageously applied for examination of captopril from Caco-2 cell monolayers.
Une nouvelle inscription de Tamuda et le contexte militaro-politique de la province de Maurétanie Tingitane
Authors: Bernard Gwladys 
Published: January 2021

Une inscription découverte récemment à Tamuda et publiée en 2018 mentionne des ennemis mis en fuite (fugatis • họ[stibus]) et un procurateur, aussi qualifié de praeses, dont le nom a été martelé. Les interprétations des premiers éditeurs de l’inscription concernant le formulaire et la datation font ici l’objet d’un nouvel examen, afin de comprendre le contexte militaire et politique de la province de Maurétanie Tingitane au iiie siècle.