bluuebunny/crossref_metadata_2025_split huggingface dataset:
Characterisation of vaping liquids used in vaping devices across four countries: results from an analysis of selected vaping liquids reported by users in the 2016 ITC Four Country Smoking and Vaping Survey
Authors: Fix Brian Vincent , OConnor Richard J , Goniewicz Maciej Lukasz , Leigh Noel L , Cummings Michael , Hitchman Sara C , Fong Geoffrey T , el Nahas Georges , Hammond David , McNeill Ann , Borland Ron , King Bill , Palumbo Mary N 
Published: May 2021

ObjectivesThis study presents an analysis of vaping products (VPs) purchased in the USA, Canada, England and Australia and assesses whether differences in regulations were associated with differences in the chemical composition of the VPs.MethodsApril–September 2017, a total of 234 VP refill liquids and prefilled cartridges were purchased in convenience samples of retail locations in each country. Products were chosen from brands and styles most commonly reported by current VP users in the 2016 ITC Four Country Smoking and Vaping Survey. All products were tested for nicotine, tobacco-specific nitrosamines (TSNAs), minor tobacco alkaloids, organic acids and flavouring chemicals.ResultsConsistent with the laws in Canada and Australia at the time of product purchase, nicotine was not detected in any of the VPs (n=10 in Canada; n=15 in Australia). US liquids (n=54) had a mean nicotine concentration of 16.2 mg/mL, (range=0.0–58.6) and English liquids (n=166) had a mean concentration of 11.9 mg/mL ((range=0.0–31.2) F(3244)=12.32, p<0.001). About 5% of English samples exceeded the UK’s 20 mg/mL nicotine limit. Substantial country differences were observed in levels ofTSNAs, with the USA being higher than elsewhere. Of all products tested, 18.8% contained at least one organic acid. Liquids purchased in England contained far more identifiable flavouring chemicals than those in the other countries.ConclusionsVP composition, particularly with respect to nicotine and flavouring, varies by country, likely reflecting both marketplace preferences and country-specific regulations. There are differences between nicotine levels claimed on the package and actual levels, particularly in England.
Gravitational waves from colliding vacuum bubbles in gauge theories
Authors: Lewicki Marek , Vaskonen Ville 
Published: May 2021

AbstractWe study production of gravitational waves (GWs) in strongly supercooled cosmological phase transitions in gauge theories. We extract from two-bubble lattice simulations the scaling of the GW source, and use it in many-bubble simulations in the thin-wall limit to estimate the resulting GW spectrum. We find that in presence of the gauge field the GW source decays with bubble radius as $$\propto R^{-3}$$

∝

R

-
3

after collisions. This leads to a GW spectrum that follows $$\Omega _{\mathrm{GW}} \propto \omega ^{2.3}$$

Ω
GW

∝

ω

2.3

at low frequencies and $$\Omega _{\mathrm{GW}} \propto \omega ^{-2.9}$$

Ω
GW

∝

ω

-
2.9

at high frequencies, marking a significant deviation from the popular envelope approximation.
Estimating Distances in Action Space in Augmented Reality
Authors: Gagnon Holly C. , Rosales Carlos Salas , Mileris Ryan , Stefanucci Jeanine K. , Creem-Regehr Sarah H. , Bodenheimer Robert E. 
Published: May 2021

Augmented reality
(
AR
) is important for training complex tasks, such as navigation, assembly, and medical procedures. The effectiveness of such training may depend on accurate spatial localization of AR objects in the environment. This article presents two experiments that test egocentric distance perception in augmented reality within and at the boundaries of action space (up to 35 m) in comparison with distance perception in a matched
real-world
(
RW
) environment. Using the Microsoft HoloLens, in Experiment 1, participants in two different RW settings judged egocentric distances (ranging from 10 to 35 m) to an AR avatar or a real person using a visual matching measure. Distances to augmented targets were underestimated compared to real targets in the two indoor, RW contexts. Experiment 2 aimed to generalize the results to an absolute distance measure using verbal reports in one of the indoor environments. Similar to Experiment 1, distances to augmented targets were underestimated compared to real targets. We discuss these findings with respect to the importance of methodologies that directly compare performance in real and mediated environments, as well as the inherent differences present in mediated environments that are “matched” to the real world.
On the Computation of Some Topological Descriptors to Find Closed Formulas for Certain Chemical Graphs
Authors: Aftab Muhammad Haroon , Rafaqat Muhammad , Hussain M. , Zia Tariq 
Published: May 2021

In this research paper, we will compute the topological indices (degree based) such as the ordinary generalized geometric-arithmetic (OGA) index, first and second Gourava indices, first and second hyper-Gourava indices, general Randic´ index

R

γ

G

,
for
γ
=

±
1
,
±

1
/
2

, harmonic index, general version of the harmonic index, atom-bond connectivity (ABC) index, SK, SK1, and SK2 indices, sum-connectivity index, general sum-connectivity index, and first general Zagreb and forgotten topological indices for various types of chemical networks such as the subdivided polythiophene network, subdivided hexagonal network, subdivided backbone DNA network, and subdivided honeycomb network. The discussion on the aforementioned networks will give us very remarkable results by using the aforementioned topological indices.
Tobacco Smoking and the Fecal Microbiome in a Large, Multi-ethnic Cohort
Authors: Prakash Ajay , Peters Brandilyn A. , Cobbs Emilia , Beggs Dia , Choi Heesun , Li Huilin , Hayes Richard B. , Ahn Jiyoung 
Published: May 2021

AbstractBackground:Increasing evidence suggests that tobacco smoking, a well-known driver of carcinogenesis, influences the gut microbiome; however, these relationships remain understudied in diverse populations. Thus, we performed an analysis of smoking and the gut microbiome in a subset of 803 adults from the multi-ethnic NYU FAMiLI study.Methods:We assessed fecal microbiota using 16S rRNA gene sequencing, and clustered samples into Amplicon Sequence Variants using QIIME2. We evaluated inferred microbial pathway abundance using PICRUSt. We compared population β-diversity, and relative taxonomic and functional pathway abundance, between never smokers, former smokers, and current smokers.Results:We found that the overall composition of the fecal microbiome in former and current smokers differs significantly from that of never smokers. The taxa Prevotella and Veillonellaceae were enriched in current and former smokers, whereas the taxa Lachnospira and Tenericutes were depleted, relative to never smokers. These shifts were consistent across racial and ethnic subgroups. Relative to never smokers, the abundance of taxa enriched in current smokers were positively correlated with the imputed abundance of pathways involving smoking-associated toxin breakdown and response to reactive oxygen species (ROS).Conclusions:Our findings suggest common mechanisms of smoking associated microbial change across racial subgroups, regardless of initial microbiome composition. The correlation of these differentials with ROS exposure pathways may suggest a role for these taxa in the known association between smoking, ROS and carcinogenesis.Impact:Smoking shifts in the microbiome may be independent of initial composition, stimulating further studies on the microbiome in carcinogenesis and cancer prevention.
Epigenetic Biomarkers of Prenatal Tobacco Smoke Exposure Are Associated with Gene Deletions in Childhood Acute Lymphoblastic Leukemia
Authors: Xu Keren , Li Shaobo , Whitehead Todd P. , Pandey Priyatama , Kang Alice Y. , Morimoto Libby M. , Kogan Scott C. , Metayer Catherine , Wiemels Joseph L. , de Smith Adam J. 
Published: May 2021

Abstract

Background:
Parental smoking is implicated in the etiology of acute lymphoblastic leukemia (ALL), the most common childhood cancer. We recently reported an association between an epigenetic biomarker of early-life tobacco smoke exposure at the AHRR gene and increased frequency of somatic gene deletions among ALL cases.

Methods:
Here, we further assess this association using two epigenetic biomarkers for maternal smoking during pregnancy—DNA methylation at AHRR CpG cg05575921 and a recently established polyepigenetic smoking score—in an expanded set of 482 B-cell ALL (B-ALL) cases in the California Childhood Leukemia Study with available Illumina 450K or MethylationEPIC array data. Multivariable Poisson regression models were used to test the associations between the epigenetic biomarkers and gene deletion numbers.

Results:
We found an association between DNA methylation at AHRR CpG cg05575921 and deletion number among 284 childhood B-ALL cases with MethylationEPIC array data, with a ratio of means (RM) of 1.31 [95% confidence interval (CI), 1.02–1.69] for each 0.1 β value reduction in DNA methylation, an effect size similar to our previous report in an independent set of 198 B-ALL cases with 450K array data [meta-analysis summary RM (sRM) = 1.32; 95% CI, 1.10–1.57]. The polyepigenetic smoking score was positively associated with gene deletion frequency among all 482 B-ALL cases (sRM = 1.31 for each 4-unit increase in score; 95% CI, 1.09–1.57).

Conclusions:
We provide further evidence that prenatal tobacco-smoke exposure may influence the generation of somatic copy-number deletions in childhood B-ALL.

Impact:
Analyses of deletion breakpoint sequences are required to further understand the mutagenic effects of tobacco smoke in childhood ALL.
Stromal-Derived Extracellular Vesicles Suppress Proliferation of Bone Metastatic Cancer Cells Mediated by ERK2
Authors: Shupp Alison B. , Neupane Manish , Agostini Lebaron C. , Ning Gang , Brody Jonathan R. , Bussard Karen M. 
Published: May 2021

Abstract

Bone is a common site of cancer metastasis, including cancers such as breast, prostate, and multiple myeloma. Disseminated tumor cells (DTC) shed from a primary tumor may travel to bone and can survive undetected for years before proliferating to form overt metastatic lesions. This period of time can be defined as metastatic latency. Once in the metastatic microenvironment, DTCs engage in intercellular communication with surrounding stromal cells, which can influence cancer cell survival, proliferation, and ultimately disease progression. The role of the surrounding tumor microenvironment in regulating DTC fate is becoming increasingly recognized. We have previously shown that in the bone microenvironment, osteoblasts are “educated” by interactions with breast cancer cells, and these “educated” osteoblasts (EO) produce soluble factors that regulate cancer cell proliferation. In this study, we provide evidence indicating that EOs produce small extracellular vesicles (sEV) that suppress breast cancer proliferation, in part through regulation of ERK1/2 signaling. In addition, using EdU-incorporation assays and propidium iodide staining we demonstrate that exposure to EO-derived sEVs decreases breast cancer cell entry to S-phase of cell cycle. We also have evidence that particular microRNAs, including miR-148a-3p, are enriched in EO-derived sEVs, and that miR-148a-3p is capable of regulating breast cancer proliferation.

Implications:
These findings underscore the importance of sEV-mediated communication in the earlier stages of cancer progression, and suggest that EO-derived sEVs may be one mechanism by which the bone microenvironment suppresses breast cancer cell proliferation.
GSDMD-Mediated Cardiomyocyte Pyroptosis Promotes Myocardial I/R Injury
Authors: Shi Huairui , Gao Yang , Dong Zhen , Yang Ji’e , Gao Rifeng , Li Xiao , Zhang Shuqi , Ma Leilei , Sun Xiaolei , Wang Zeng , Zhang Feng , Hu Kai , Sun Aijun , Ge Junbo 
Published: May 2021

Rationale:
Pyroptosis is a morphologically and mechanistically distinct form of cell death and is characterized by GSDMD (gasdermin D) or GSDME (gasdermin E)-mediated necrosis with excessive inflammatory factor release. Cardiomyocyte necrosis and inflammation play key roles in the pathophysiology of myocardial ischemia/reperfusion (I/R) injury. However, whether cardiomyocytes undergo pyroptosis and the underlying mechanism in myocardial I/R injury remain unclear.

Objective:
We aimed to investigate the role of pyroptosis in myocardial I/R injury.

Methods and Results:
In vivo and in vitro experiments were used to investigate pyroptosis of cardiomyocyte and the associated mechanisms during I/R injury. Wild-type, Myh6-Cre, and cardiomyocyte-specific GSDMD-deficient male mice were subjected to I/R. Human peripheral blood samples were collected from patients with acute ST-segment–elevation myocardial infarction or control patients at 0, 1, and 24 hours after percutaneous coronary intervention in our department. The serum levels of GSDMD were measured by ELISA. Hypoxia/reoxygenation induced cardiomyocyte pyroptosis and the release of mature IL (interleukin)-18 but not IL-1β, which mechanistically resulted from GSDMD cleavage by caspase-11 in cardiomyocytes. Furthermore, GSDMD gene deletion blocked hypoxia/reoxygenation-induced cardiomyocyte pyroptosis and IL-18 release. GSDMD and its pyroptosis-inducing N-terminal fragment were upregulated in myocardial tissues after I/R injury. Immunofluorescence analysis showed that GSDMD was mainly localized in cardiomyocytes. GSDMD deficiency in cardiomyocytes significantly reduced the I/R-induced myocardial infarct size. Moreover, increased GSDMD serum levels were detected in patients exhibiting I/R injury 1 hour after percutaneous coronary intervention for ST-segment–elevation myocardial infarction.

Conclusions:
Our results show that GSDMD-mediated cardiomyocyte pyroptosis is a key event during myocardial I/R injury and that the caspase-11/GSDMD pathway may be essential to this process. Additionally, GSDMD inhibition significantly reduces cardiomyocyte pyroptosis and I/R-induced myocardial injury.
Decoding of EEG Signals Shows No Evidence of a Neural Signature for Subitizing in Sequential Numerosity
Authors: Fornaciai Michele , Park Joonkoo 
Published: May 2021

Abstract
Numerosity perception is largely governed by two mechanisms. The first so-called subitizing system allows one to enumerate a small number of items (up to three or four) without error. The second system allows only an approximate estimation of larger numerosities. Here, we investigate the neural bases of the two systems using sequentially presented numerosity. Sequential numerosity (i.e., the number of events presented over time) starts as a subitizable set but may eventually transition into a larger numerosity in the approximate estimation range, thus offering a unique opportunity to investigate the neural signature of that transition point, or subitizing boundary. If sequential numerosity is encoded by two distinct perceptual mechanisms (i.e., for subitizing and approximate estimation), neural representations of the sequentially presented items crossing the subitizing boundary should be sharply distinguishable. In contrast, if sequential numerosity is encoded by a single perceptual mechanism for all numerosities and subitizing is achieved through an external postperceptual mechanism, no such differences in the neural representations should indicate the subitizing boundary. Using the high temporal resolution of the EEG technique incorporating a multivariate decoding analysis, we found results consistent with the latter hypothesis: No sharp representational distinctions were observed between items across the subitizing boundary, which is in contrast with the behavioral pattern of subitizing. The results support a single perceptual mechanism encoding sequential numerosities, whereas subitizing may be supported by a postperceptual attentional mechanism operating at a later processing stage.
First and Second Language at Hand: A Chronometric Transcranial-Magnetic Stimulation Study on Semantic and Motor Resonance
Authors: Monaco Elisa , Jost Lea B. , Lancheros Monica , Harquel Sylvain , Schmidlin Eric , Annoni Jean-Marie 
Published: May 2021

Abstract
According to embodied theories, motor and language processing bidirectionally interact: Motor activation modulates behavior in lexico-semantic tasks (semantic resonance), and understanding motor-related words entails activation of the corresponding motor brain areas (motor resonance). Whereas many studies investigated such interaction in the first language (L1), only few did so in a second language (L2), focusing on motor resonance. Here, we directly compared L1 and a late L2, for the first time both in terms of semantic and motor resonance and both in terms of magnitude and timing, by taking advantage of single-pulse TMS. Twenty-five bilinguals judged, in each language, whether hand motor-related (“grasp”) and non-motor-related verbs (“believe”), were physical or mental. Meanwhile, we applied TMS on the hand motor cortex at 125, 275, 350, and 500 msec post verb onset, and recorded behavioral responses and TMS-induced motor evoked potentials. TMS induced faster responses for L1 versus L2 motor and nonmotor verbs at 125 msec (three-way interaction β = −0.0442, 95% CI [0.0814, −0.0070]), showing a semantic resonance effect at an early stage of word processing in L1 but not in L2. Concerning motor resonance, TMS-induced motor evoked potentials at 275 msec revealed higher motor cortex excitability for L2 versus L1 processing (two-way interaction β = 0.095, 95% CI [0.017, 0.173]).
These findings confirm action–language interaction at early stages of word recognition, provide further evidence that L1 and L2 are differently embodied, and call for an update of existing models of bilingualism and embodiment, concerning both language representations and processing.
The Effect of Familiarity on Neural Representations of Music and Language
Authors: Sternin Avital , McGarry Lucy M. , Owen Adrian M. , Grahn Jessica A. 
Published: May 2021

Abstract
We investigated how familiarity alters music and language processing in the brain. We used fMRI to measure brain responses before and after participants were familiarized with novel music and language stimuli. To manipulate the presence of language and music in the stimuli, there were four conditions: (1) whole music (music and words together), (2) instrumental music (no words), (3) a capella music (sung words, no instruments), and (4) spoken words. To manipulate participants' familiarity with the stimuli, we used novel stimuli and a familiarization paradigm designed to mimic “natural” exposure, while controlling for autobiographical memory confounds. Participants completed two fMRI scans that were separated by a stimulus training period. Behaviorally, participants learned the stimuli over the training period. However, there were no significant neural differences between the familiar and unfamiliar stimuli in either univariate or multivariate analyses. There were differences in neural activity in frontal and temporal regions based on the presence of language in the stimuli, and these differences replicated across the two scanning sessions. These results indicate that the way we engage with music is important for creating a memory of that music, and these aspects, over and above familiarity on its own, may be responsible for the robust nature of musical memory in the presence of neurodegenerative disorders such as Alzheimer disease.
Very rare heat extremes: quantifying and understanding using ensemble re-initialization
Authors: Gessner Claudia , Fischer Erich M. , Beyerle Urs , Knutti Reto 
Published: May 2021

AbstractHeat waves such as the one in Europe 2003 have severe consequences for the economy, society, and ecosystems. It is unclear whether temperatures could have exceeded these anomalies even without further climate change. Developing storylines and quantifying highest possible temperature levels is challenging given the lack of long homogeneous time series and methodological framework to assess them. Here, we address this challenge by analysing summer temperatures in a nearly 5000-year pre-industrial climate model simulation, performed with the Community Earth System Model CESM1. To assess how anomalous temperatures could get, we compare storylines, generated by three different methods: (1) a return-level estimate, deduced from a generalized extreme value distribution, (2) a regression model, based on dynamic and thermodynamic heat wave drivers, and (3) a novel ensemble boosting method, generating large samples of re-initialized extreme heat waves in the long climate simulation.All methods provide consistent temperature estimates, suggesting that historical exceptional heat waves as in Chicago 1995, Europe 2003 and Russia 2010 could have been substantially exceeded even in the absence of further global warming. These estimated unseen heat waves are caused by the same drivers as moderate observed events, but with more anomalous patterns. Moreover, altered contributions of circulation and soil moisture to temperature anomalies include amplified feedbacks in the surface energy budget. The methodological framework of combining different storyline approaches of heat waves with magnitudes beyond the observational record may ultimately contribute to adaptation and to the stress testing of ecosystems or socio-economic systems to increase resilience to extreme climate stressors.
Super Residual Circulation: a new perspective on ocean vertical heat transport
Authors: Savita Abhishek , Zika Jan D. , Domingues Catia M. , Marsland Simon J. , Evans Gwyn Dafydd , Dias Fabio Boeira , Holmes Ryan M. , Hogg Andrew McC. 
Published: May 2021

AbstractOcean circulation and mixing regulate Earth’s climate by moving heat vertically within the ocean. We present a new formalism to diagnose the role of ocean circulation and diabatic processes in setting vertical heat transport in ocean models. In this formalism we use temperature tendencies, rather than explicit vertical velocities to diagnose circulation. Using quasi-steady state simulations from the Australian Community Climate and Earth-System Simulator Ocean Model (ACCESS-OM2), we diagnose a diathermal overturning circulation in temperature-depth space. Furthermore, projection of tendencies due to diabatic processes onto this coordinate permits us to represent these as apparent overturning circulations. Our framework permits us to extend the concept of Super-Residual Transport (SRT), which combines mean and eddy advection terms with subgridscale isopycnal mixing due to mesoscale eddies, but excludes small-scale three dimensional turbulent mixing effect, to construct a new overturning circulation – the ‘Super Residual Circulation’ (SRC).We find that in the coarse resolution version of ACCESS-OM2 (nominally 1° horizontal resolution) the SRC is dominated by an ~11 Sv circulation which transports heat upward. The SRC’s upward heat transport is ~2 times larger in a finer horizontal resolution (0.1°) version of ACCESS, suggesting a differing balance of super-residual and parameterized small-scale processes may emerge as eddies are resolved. Our analysis adds new insight into super-residual processes, as the SRC elucidates the pathways in temperature and depth space along which watermass transformation occurs.