bluuebunny/crossref_metadata_2025_split huggingface dataset:
ENDOMETRIOSIS-ASSOCIATED INFERTILITY: THE ROLE OF HORMONES AND ITS CORRECTION
Authors: Kulyk I. I. , Khmil S. V. 
Published: June 2021

Background. Endometriosis-associated infertility (EAI) has a number of specific features, which are crucial in the choice of medical treatment.
Objective. The aim of the study is to analyze endocrine profile in women with EAI before and after sclerotherapy and pregravid preparation (PP), which includes a vitamin complex FT 500 plus with inositol and vitamin D3.
Methods. The study involved 70 women aged 21-40 years with endometriosis-associated infertility. The comparison group included 30 women with tuboperitoneal infertility. ELISA was used to determine concentrations of Anti-Mullerian hormone (AMH), follicle stimulating hormone (FSH), luteinizing hormone (LH), progesterone and estradiol in blood serum using a standard kit by Diagnostic Systems Laboratories, Inc (USA). During two menstrual cycles the FT 500 plus was prescribed once a day from the 2nd/3rd day of the cycle, vitamin D3 was prescribed at the dose of 2,000 IU for women without its deficiency and in therapeutic doses in case of hypovitaminosis. The sclerotherapy with 95% ethanol solution was performed on the 6th-8th day of menstrual cycle.
Results. It was established that in women with EAI undergoing PP and sclerotherapy the level of AMH was lower (by 12.90%) as well as progesterone (by 9.84%), while FSH (by 14.47%), LH (by 21.14%) and estradiol (by 35.55%) was higher compare to the comparison group. At the same time, FSH (by 21.98%), LH (by 32.89%) and estradiol (by 32.23%) concentrations were significantly lower compare to their primary indices before sclerotherapy.
Conclusions. Sclerotherapy and PP with a vitamin complex, inositol and vitamin D3 has a positive effect on endocrine profile in women with endometriosis-associated infertility
QUALITY CONTROL MEASUREMENT AND IN VITRO BIOEQUIVALENCE OF VALSARTAN AND ATENOLOL TABLETS MARKETED IN UKRAINE
Authors: Peleshok K. Y. 
Published: June 2021

Background. The urgent issue of hypertension is determined by its high population incidence, significant burden of the disease, risk of disability and impact on life expectancy. Rational combinations of drugs of different pharmacological groups in case of ineffectiveness of monotherapy to achieve the clinical effect of pharmacotherapy are clearly recommended in the world and national recommendations for diagnosis and treatment of hypertension. Therefore, innovative pharmaceutical development of a combination of antihypertensive drugs and creation of domestic drugs with antihypertensive action is an urgent task of contemporary pharmacy.
Objective. The aim of this study was to perform the quality control measurements and evaluation of dissolution tests for different brands of valsartan and atenolol tablets available in Ukraine.
Methods. The concentrations of valsartan and atenolol in samples (drug content and dissolution study) were determined by the proposed HPLC method.
Results. The results of the tests conducted for evaluation of the tablets were found to be in acceptable limits for all the selected brands. The correlation coefficient (R2) was 0.9991 and the regression equation was y=61.39x+0.3117. It has been established that the equivalence of dissolution profiles for all recommended dissolution media is observed (рН 1.2, 4.5, and 6.8) for the studied drugs. In all three dissolution media, the release rates of valsartan and atenolol of all dosage forms are more than 85% in 15 min. The dissolution profile of all the selected brands was within the standard limits and was acceptable.
Conclusions. Analytical method development is an integral part of the quality control measurements and evaluation of dissolution tests. Our previously developed HPLC method is essential for quality control of a large number of samples in short time intervals. Therefore, the method developed by our group is suitable for a routine quality control analysis of any pharmaceutical preparation containing two tested drugs with the suggested chromatographic method advantages for checking quality during dissolution studies of their dosage forms.
ВПЛИВ МЕЛАТОНІНУ НА СТАН ОКСИДАНТНОЇ ТА АНТИОКСИДАНТНОЇ СИСТЕМ І РІВЕНЬ ГІДРОГЕН СУЛЬФІДУ В КРОВІ ЩУРІВ ЗА УМОВ ЕКСПЕРИМЕНТАЛЬНОЇ НЕФРОПАТІЇ
Authors: Коляник І. О. , Геруш І. В. , Григор’єва Н. П. 
Published: June 2021

Вступ. Окиснювальний стрес залучений у патогенез різноманітних захворювань, включаючи гостре і хронічне ушкодження нирок, та характеризується підвищенням внутрішньоклітинного рівня активних форм оксигену. Це відіграє важливу роль у розвитку нефропатії та є потенційною ціллю для терапевтичного втручання. В останні роки актуальне також вивчення ролі Н2S у підтриманні окисно-відновного гомеостазу та впливу на активність антиоксидантного захисту. Одним із найбільш поширених антиоксидантів є мелатонін. Він здатний перехоплювати вільні радикали та стимулювати активність антиоксидантних ензимів. Однак залишаються нез’ясованими механізми його впливу на вміст Н2S та антиоксидантну систему крові при нефропатії.
Мета дослідження – з’ясувати вплив мелатоніну на стан біохімічних показників, оксидантної та антиоксидантої систем і рівень гідроген сульфіду в крові щурів за умов експериментальної нефропатії.
Методи дослідження. Експеримент проведено на 127 нелінійних щурах-самцях масою 0,16–0,18 кг. Експериментальну нефропатію моделювали шляхом одноразового внутрішньочеревного введення фоліє­вої кислоти в дозі 250 мг/кг маси тіла. Мелатонін вводили інтрагастрально впродовж 3 та 7 днів після моделювання нефропатії в дозі 10 мг/кг. У плазмі крові визначали вміст сечовини, креатиніну, ТБК-активних продуктів, карбонільних похідних протеїнів нейтрального (ОМП370) та основного (ОМП430) характеру, церулоплазміну, SH-груп і Н2S, у гемолізаті еритроцитів – активність каталази, супероксиддисмутази (SOD), глутатіонпероксидази (GPx) та глутатіон-S-трансферази (GST).
Результати й обговорення. За умов нефропатії у крові щурів спостерігали підвищення концентрації сечовини, креатиніну, вмісту ТБК-активних продуктів, ОМБ370, ОМБ430, церулоплазміну та зниження активності антиоксидантних ензимів, таких, як каталаза, SOD, GPx, GST, а також вмісту SH-груп і Н2S. Ці зміни свідчили про порушення функціонального стану нирок, розвиток окиснювального стресу та виснаження антиоксидантного захисту. Введення мелатоніну впродовж 7 днів сприяло нормалізації показників, що характеризували функціональний стан нирок, оксидантний стан крові, та мало позитивний вплив на активність каталази, GPx, GST, рівень церулоплазміну, вміст SH-груп і Н2S.
Висновок. Введення екзогенного мелатоніну в дозі 10 мг/кг упродовж 3 і 7 днів знижує прояви нефротичного синдрому та вільнорадикального окиснення біомолекул, а також має нормалізуючий вплив на антиоксидантну систему і вміст Н2S, що, ймовірно, зумовлено його здатністю нейтралізувати вільні радикали та активувати антиоксидантні ензими.
РОЗРОБКА МЕТОДИКИ ІДЕНТИФІКАЦІЇ МОНОСАХАРИДІВ У РІДКОМУ ЕКСТРАКТІ ЧЕБРЕЦЮ ПОВЗУЧОГО
Authors: Зарівна Н. О. 
Published: June 2021

Вступ. Пріоритетним напрямком сучасної фармації є пошук і створення нових лікарських засобів на основі лікарської рослинної сировини. Фармацевтична розробка на основі густого екстракту чебрецю повзучого перш за все передбачала одержання рідкого екстракту даної рослини, який отримано за технологією, що дозволила максимально зберегти якісний склад біологічно активних речовин відповідної лікарської рослинної сировини. Оскільки при стандартизації трави чебрецю повзучого ідентифікаційними маркерами якості серед інших обрано моносахариди, доречним є вивчення якісного складу цих біологічно активних речовин і в досліджуваному екстракті, адже для них характерний широкий спектр фармакологічної дії.
Мета дослідження – розробити методику ідентифікації моносахаридів у рідкому екстракті чебрецю повзучого після проведення попереднього гідролізу та обрати відповідні маркери якості.
Методи дослідження. Під час дослідження використано рідкий екстракт чебрецю повзучого, стандартні зразки речовин: арабінози, галактози, рамнози, фруктози, глюкози, ксилози (“Fluka”), хроматографічні пластинки “Silica gel” фірми “Merck”, прилад для автоматичного нанесення проб на пластинку “CAMAG Linomat 5”, хроматографічну камеру “GAMAG”, УФ-лампу, метод тонкошарової хроматографії.
Результати й обговорення. Для стандартизації рідкого екстракту чебрецю повзучого було розглянуто можливість ідентифікації аналогічних біологічно активних речовин сировини, продовжуючи запропонований підхід: лікарська рослинна сировина – екстракти – лікарський засіб. Якісний склад моносахаридів визначали методом тонкошарової хроматографії в системі розчинників вода Р – ацетонітрил Р у співвідношенні 15:85. Для розробки методики якісного визначення аналізованих біологічно активних речовин у рідкому екстракті чебрецю повзучого враховували різні способи та умови хроматографування, в результаті чого було підібрано оптимальну систему розчинників з хорошою роздільною здатністю, за допомогою якої проведено ідентифікацію моносахаридів у досліджуваному екстракті.
Висновки. Розроблено методику якісного визначення моносахаридів у рідкому екстракті чебрецю повзучого. У результаті дослідження ідентифіковано 7 моносахаридів, один з яких залишився невідомим у зв’язку з відсутністю необхідного стандарту, і запропоновано маркери якості досліджуваного екстракту (наявність фруктози та глюкози).
Characteristics and Outcomes of Women Developing Heart Failure After Early Stage Breast Cancer Chemotherapy: A Population-Based Matched Cohort Study
Authors: Abdel-Qadir Husam , Tai Felicia , Croxford Ruth , Austin Peter C. , Amir Eitan , Calvillo-Argüelles Oscar , Ross Heather , Lee Douglas S. , Thavendiranathan Paaladinesh 
Published: June 2021

Background:
The prognosis of heart failure (HF) after early stage breast cancer (EBC) treatment with anthracyclines or trastuzumab is not well-characterized.

Methods:
Using administrative databases, women diagnosed with HF after receiving anthracyclines or trastuzumab for EBC in Ontario during 2007 to 2017 (the EBC-HF cohort) were categorized by cardiotoxic exposure (anthracycline alone, trastuzumab alone, sequential therapy with both agents) and matched on age with ≤3 cancer-free HF controls to compare baseline characteristics. To study prognosis after HF onset, we conducted a second match on age plus important HF prognostic factors. The cumulative incidence function was used to describe risk of hospitalization or emergency department visits (hospital presentations) for HF and cardiovascular death.

Results:

A total of 804 women with EBC developed HF after anthracyclines (n=312), trastuzumab (n=112), or sequential therapy (n=380); they had significantly fewer comorbidities than 2411 age-matched HF controls. After the second match, the anthracycline-HF cohort had a similar 5-year incidence of HF hospital presentations (16.5% [95% CI, 12.0%–21.7%]) as controls (17.1% [95% CI, 14.4%–20.1%]); the 5-year incidence was lower than matched controls for the trastuzumab-HF (9.7% [95% CI, 4.7%–16.9%]; controls 16.4% [95% CI, 12.1%–21.3%];
P
=0.03) and sequential-HF cohorts (2.7% [95% CI, 1.4%–4.8%]; controls 10.8% [95% CI, 8.9%–13.0%];
P
<0.001). At 5 years, the incidence of cardiovascular death was 2.9% (95% CI, 1.2%–5.9%) in the anthracycline-HF cohort vs. 9.5% (95% CI, 6.9%–12.6%) in controls, and 1.7% (0.6%–3.7%) for women developing HF after trastuzumab vs. 4.3% (95% CI, 3.1–5.8%) for controls.

Conclusions:
Women developing HF after cardiotoxic EBC chemotherapy have fewer comorbidities than cancer-free women with HF; trastuzumab-treated women who develop HF have better prognosis than matched HF controls.
Multiparametric Implantable Cardioverter-Defibrillator Algorithm for Heart Failure Risk Stratification and Management: An Analysis in Clinical Practice
Authors: Calò Leonardo , Bianchi Valter , Ferraioli Donatella , Santini Luca , Dello Russo Antonio , Carriere Cosimo , Santobuono Vincenzo Ezio , Andreoli Chiara , La Greca Carmelo , Arena Giuseppe , Talarico Antonello , Pisanò Ennio , Santoro Amato , Giammaria Massimo , Ziacchi Matteo , Viscusi Miguel , De Ruvo Ermenegildo , Campari Monica , Valsecchi Sergio , D’Onofrio Antonio , Minati M , Tota C , Martino A , Tavoletta V , Manzo M , Ammirati F , Mahfouz K , Colaiaco C , Guerra F , Zorzin Fantasia A , Amato V , Savarese G , Pellegrini D , Pimpinicchio L , Pecora D , Bartoli C , Borrello V.M , Ratti M , De Rosa F , Quirino F , Tomaselli C , Marino E , Baiocchi C , De Vivo O , Baccani B , Amellone C , Lucciola M.T , Angeletti A , Frisoni J , Brignoli M , Costa A , Pangallo A , Benedetto F , Pepi P , Nicolis D , Petracci B , Giubilato G , Carbonardi L , Porcelli D , Romani B , Zuccaro L.M. 
Published: June 2021

Background:
The HeartLogic algorithm combines multiple implantable cardioverter-defibrillator sensors to identify patients at risk of heart failure (HF) events. We sought to evaluate the risk stratification ability of this algorithm in clinical practice. We also analyzed the alert management strategies adopted in the study group and their association with the occurrence of HF events.

Methods:
The HeartLogic feature was activated in 366 implantable cardioverter-defibrillator and cardiac resynchronization therapy implantable cardioverter-defibrillator patients at 22 centers. The median follow-up was 11 months [25th–75th percentile: 6–16]. The HeartLogic algorithm calculates a daily HF index and identifies periods IN alert state on the basis of a configurable threshold.

Results:

The HeartLogic index crossed the threshold value 273 times (0.76 alerts/patient-year) in 150 patients. The time IN alert state was 11% of the total observation period. Patients experienced 36 HF hospitalizations, and 8 patients died of HF during the observation period. Thirty-five events were associated with the IN alert state (0.92 events/patient-year versus 0.03 events/patient-year in the OUT of alert state). The hazard ratio in the IN/OUT of alert state comparison was (hazard ratio, 24.53 [95% CI, 8.55–70.38],
P
<0.001), after adjustment for baseline clinical confounders. Alerts followed by clinical actions were associated with less HF events (hazard ratio, 0.37 [95% CI, 0.14–0.99],
P
=0.047). No differences in event rates were observed between in-office and remote alert management.

Conclusions:
This multiparametric algorithm identifies patients during periods of significantly increased risk of HF events. The rate of HF events seemed lower when clinical actions were undertaken in response to alerts. Extra in-office visits did not seem to be required to effectively manage HeartLogic alerts.

Registration:

URL:
https://www.clinicaltrials.gov
; Unique identifier: NCT02275637.
Electronic and Tobacco Cigarettes Alter Polyunsaturated Fatty Acids and Oxidative Biomarkers
Authors: Gupta Rajat , Lin Yan , Luna Karla , Logue Anjali , Yoon Alexander J. , Haptonstall Kacey P. , Moheimani Roya , Choroomi Yasmine , Nguyen Kevin , Tran Elizabeth , Zhu Yifang , Faull Kym F. , Kelesidis Theodoros , Gornbein Jeffrey , Middlekauff Holly R. , Araujo Jesus A. 
Published: June 2021

Rationale:
Chronic electronic cigarette (EC) users exhibit a higher susceptibility of LDL (low-density lipoprotein) to undergo oxidation as compared with nonuser controls. However, there is a paucity of data regarding EC effects on lipid peroxidation in the blood and their relationship to cardiovascular risk.

Objective:
To test the hypothesis that chronic (≥1 year) EC use exerts intermediate effects on plasma lipid peroxidation and/or antioxidant defense compared with chronic tobacco cigarette (TC) smoking.

Methods and Results:

We enrolled EC-users (n=32), TC-smokers (n=29), and nonusers (n=45), with mean ages of 28.3, 27.8, and 27.4 years, respectively. Plasma concentrations of free polyunsaturated fatty acids and oxidized metabolites were assessed by mass spectrometry. Total antioxidant capacity, concentrations of glutathione, bilirubin, HO-1 (heme oxygenase-1), and functional activity of PON1 (paraoxonase1) were determined by colorimetric and enzymatic assays. Multivariable analysis was performed using classification models for segregating participants based on biomarker profiles. TC-smokers exhibited higher plasma arachidonic acid concentration. Instead, EC-users displayed lower plasma concentrations of both arachidonic acid and linoleic acid as compared to non-users and TC-smokers (
P
<0.05). Oxidized linoleic acid metabolites (9- and 13-hydroxyoctadecadienoic acid) were lower in EC-users and TC-smokers as compared with nonusers (
P
<0.001). Consistently, total antioxidant capacity and bilirubin were elevated in EC-users and TC-smokers as compared with nonusers (
P
<0.05). Of interest, plasma HO-1 concentration was higher in TC-smokers as compared with nonusers (
P
=0.01) with intermediate levels in EC-users. Multivariable analysis identified 5 biomarkers (13-hydroxyoctadecadienoic acid, linoleic acid, 9-hydroxyoctadecadienoic acid, 12-hydroxyeicosatetraenoic acid, arachidonic acid) that discriminated EC-users from TC-smokers and nonusers with an accuracy of 73.4%.

Conclusions:
Chronic use of EC induces common (ie, lower 9- and/or 13-hydroxyoctadecadienoic acids and higher total antioxidant capacity and bilirubin) as well as differential effects (ie, altered arachidonic acid and linoleic acid concentrations) to those induced by TC, along with intermediate plasma HO-1 concentration, suggesting that EC, likewise TC smoke, could impact cardiovascular risk.
Impact of Midregional N‐Terminal Pro–Atrial Natriuretic Peptide and Soluble Suppression of Tumorigenicity 2 Levels on Heart Rhythm in Patients Treated With Catheter Ablation for Atrial Fibrillation: The Biorhythm Study
Authors: Badoz Marc , Serzian Guillaume , Favoulet Baptiste , Sellal Jean‐Marc , De Chillou Christian , Hammache Néfissa , Laurent Gabriel , Mebazaa Alexandre , Ecarnot Fiona , Bardonnet Karine , Seronde Marie‐France , Schiele François , Meneveau Nicolas 
Published: June 2021

Background
We assessed the impact of preprocedural plasma levels of MRproANP (midregional N‐terminal pro–atrial natriuretic peptide) and sST2 (soluble suppression of tumorigenicity 2) on recurrence of atrial fibrillation (AF) at 1 year after catheter ablation of AF.

Methods and Results

This was a prospective, multicenter, observational study including patients undergoing catheter ablation of AF. MRproANP and sST2 were measured in a peripheral venous blood preprocedure, and MRproANP was assessed in the right and left atrial blood during ablation. The primary end point was recurrent AF between 3 and 12 months postablation, defined as a documented (>30 seconds) episode of AF, flutter, or atrial tachycardia. We included 106 patients from December 2017 to March 2019; 105 had complete follow‐up, and the mean age was 63 years with 74.2% males. Overall, 34 patients (32.1%) had recurrent AF. In peripheral venous blood, MRproANP was significantly higher in patients with recurrent AF (median, 192.2; [quartile 1–quartile 3, 155.9–263.9] versus 97.1 [60.9–150.7] pmol/L;
P
<0.0001), as was sST2 (median, 30.3 [quartile 1–quartile 3, 23.3–39.3] versus 23.4 [95% CI, 17.4–33.0] ng/mL;
P
=0.0033). In the atria, MRproANP was significantly higher than in peripheral blood and was higher during AF than during sinus rhythm. Receiver operating characteristic curve analysis identified a threshold of MRproANP>107.9 pmol/L to predict AF recurrence at 1 year and a threshold of >26.7 ng/mL for sST2. By multivariate analysis, MRproANP>107.9 pmol/L was the only independent predictor of recurrent AF (OR, 24.27; 95% CI, 4.23–139.18). MRproANP<107.9 pmol/L identified subjects at very low risk of recurrence (negative predictive value >95%).

Conclusions
Elevated MRproANP level independently predicts recurrent AF, whereas sST2 levels do not appear to have any prognostic value in assessing the risk of recurrence of AF up to 1 year after catheter ablation.

Registration

URL:
https://www.clinicaltrials.gov
; Unique identifier: NCT03351816.
Are Artificial Dendrites Useful in Neuro-Evolution?
Authors: Bull Larry 
Published: June 2021

Abstract
The significant role of dendritic processing within neuronal networks has become increasingly clear. This letter explores the effects of including a simple dendrite-inspired mechanism into neuro-evolution. The phenomenon of separate dendrite activation thresholds on connections is allowed to emerge under an evolutionary process. It is shown how such processing can be positively selected for, particularly for connections between the hidden and output layers, and increases performance.
Neural Dynamics of Conflict Control in Working Memory
Authors: Vo Khoi D. , Siqi-Liu Audrey , Chaire Alondra , Li Sophia , Demeter Elise , Egner Tobias , Woldorff Marty G. 
Published: June 2021

Abstract
Attention and working memory (WM) have classically been considered as two separate cognitive functions, but more recent theories have conceptualized them as operating on shared representations and being distinguished primarily by whether attention is directed internally (WM) or externally (attention, traditionally defined). Supporting this idea, a recent behavioral study documented a “WM Stroop effect,” showing that maintaining a color word in WM impacts perceptual color-naming performance to the same degree as presenting the color word externally in the classic Stroop task. Here, we employed ERPs to examine the neural processes underlying this WM Stroop task compared to those in the classic Stroop and in a WM-control task. Based on the assumption that holding a color word in WM would (pre-)activate the same color representation as by externally presenting that color word, we hypothesized that the neural cascade of conflict–control processes would occur more rapidly in the WM Stroop than in the classic Stroop task. Our behavioral results replicated equivalent interference behavioral effects for the WM and classic Stroop tasks. Importantly, however, the ERP signatures of conflict detection and resolution displayed substantially shorter latencies in the WM Stroop task. Moreover, delay-period conflict in the WM Stroop task, but not in the WM control task, impacted the ERP and performance measures for the WM probe stimuli. Together, these findings provide new insights into how the brain processes conflict between internal representations and external stimuli, and they support the view of shared representations between internally held WM content and attentional processing of external stimuli.