bluuebunny/crossref_metadata_2025_split huggingface dataset:
Molecular characterization and immune protection by cystathionine β‐synthase from Eimeria tenella
Authors: Zhao Huanzhi , Li Cong , Zhu Shunhai , Zhao Qiping , Dong Hui , Huang Bing , Han Hongyu 
Published: December 2021

AbstractEimeria tenella is an obligate intracellular apicomplexan parasite that causes avian coccidiosis and leads to severe economic losses in the global poultry industry. Cystathionine β‐synthase (CBS) and cystathionine γ‐lyase (CGL) act together to generate H2S in the reverse transsulfuration pathway. In this study, E. tenella Cystathionine β‐synthase (EtCBS) was cloned using rapid amplification of cDNA 5′‐ends (5'RACE) and characterized, and its immunoprotective effects were evaluated. The recombinant EtCBS protein (rEtCBS) was expressed and successfully recognized by anti‐sporozoites (Spz) protein rabbit serum. EtCBS mRNA levels were highest in Spz by qPCR, and the protein expression levels were higher in unsporulated oocysts (UO) than in other stages by Western blot. Indirect immunofluorescence showed that EtCBS protein was found on the surface of Spz and second‐generation merozoites (Mrz). The invasion inhibition assays showed that rabbit anti‐rEtCBS polyclonal antibodies effectively inhibited parasite invasion host cells. Chickens immunized with rEtCBS protein showed prominently increased weight gains and decreased oocyst output compared to nonimmunized and infected control group. The results suggest that EtCBS could be a potential vaccine candidate against E. tenella.
ZmGRAS11, transactivated by Opaque2, positively regulates kernel size in maize
Authors: Li Ye , Ma Shuai , Zhao Qianqian , Lv Di , Wang Baobao , Xiao Ke , Zhu Jiameng , Li Suzhen , Yang Wenzhu , Liu Xiaoqing , Wang Haiyang , Zhou Xiaojin , Chen Rumei 
Published: December 2021

SummaryAlthough the genetic basis for endosperm development in maize (Zea mays) has been well studied, the mechanism for coordinating grain filling with increasing kernel size remains elusive. Here, we report that increased kernel size was selected during modern breeding and identify a novel DELLA‐like transcriptional regulator, ZmGRAS11, which positively regulates kernel size and kernel weight in maize. We find that Opaque2, a core transcription factor for zein protein and starch accumulation, transactivates the expression of ZmGRAS11. Our data suggest that the Opaque2‐ZmGRAS11 module mediates synergistic endosperm enlargement with grain filling.
(When) Are Dynamic Capabilities Routine? A Mixed‐Methods Configurational Analysis
Authors: Wilhelm Hendrik , Maurer Indre , Ebers Mark 
Published: December 2021

AbstractDynamic capabilities research is hampered by a theoretical divide. This divide concerns, first, whether dynamic capabilities are routine or not, and, second, whether firms in dynamic environments deploy routine or non‐routine dynamic capabilities. The divide is significant for theory, as it pertains to the conceptualization and boundary conditions of dynamic capabilities. The present study offers a pathway for reconciling the divide by overcoming the bifurcation of routine and non‐routine dynamic capabilities that dominates the debate. Conceptually, we relax extant assumptions on dynamic capability routineness by allowing for the possibility that dynamic capabilities may vary in dimensions of their routineness (i.e., the frequency and structuring of dynamic capability activities). Findings of a fuzzy‐set Qualitative Comparative Analysis of 103 firms operating in more and in less dynamic environments reveal four configurations of dynamic capabilities (i.e., Experimental, Adaptive, Programmed, and Analytical) that fundamentally differ in their routineness. Moreover, we find that environmental dynamism does not constitute a boundary condition for the routineness of dynamic capabilities. In‐depth analyses of 16 cases suggest that the different dynamic capability configurations we observe depend not only on environmental dynamism but also on distinct intra‐firm conditions (i.e., organizational learning orientations and resource allocations). As its main contribution, this study offers a configurational framework explaining heterogeneity in the routineness of dynamic capabilities that advances our understanding of the nature and boundary conditions of dynamic capabilities.
Is TIMP‐1 a biomarker for periodontal disease? A systematic review and meta‐analysis
Authors: de Brouwer Puk , Bikker Floris J. , Brand Henk S. , Kaman Wendy E. 
Published: December 2021

AbstractObjectiveOne of the most important families of proteases associated with periodontal disease is the family of the matrix metalloproteinases (MMPs). Their activity is regulated by tissue inhibitors of metalloproteinases (TIMPs), and an imbalance between MMP activity and regulation by TIMPs has been associated with the progression of periodontal disease. This strong interaction between TIMPs and MMPs might be an indication that TIMPs can be used as a biomarker to monitor periodontal disease progression in oral fluids. In particular, TIMP‐1 is a frequently studied biomarker for periodontal diseases. Therefore, the aim of this systematic review was to evaluate the scientific literature regarding TIMP‐1 concentrations in oral fluids of patients suffering from periodontitis or gingivitis in comparison to healthy individuals.Material and MethodsPubMed/ MedLine and Web of Science databases were searched electronically. Studies that met the inclusion criteria were systematically evaluated and assessed for eligibility and risk of bias. Meta‐analysis was performed through the random effects model to assess the association between periodontitis/gingivitis and TIMP‐1 concentration in stimulated saliva, unstimulated saliva, and gingival crevicular fluid (GCF).ResultsThe search strategy provided a total of 322 studies of which 10 studies met all inclusion criteria. Two studies investigated TIMP‐1 concentrations in GCF, three studies in unstimulated saliva, and five studies investigated TIMP‐1 concentrations in stimulated saliva. Three studies revealed that TIMP‐1 levels in oral fluids were significantly decreased in periodontal disease. Meta‐analysis revealed that there is no statistically significant difference between TIMP‐1 concentration in oral fluids of periodontitis/gingivitis patients in comparison to healthy individuals.ConclusionsThis systematic review with meta‐analysis shows that periodontal diseases are not associated with a statistically significant change in TIMP‐1 concentration in oral fluids.
French Jesuits, Origen, and Vatican II
Authors: O’Leary Joseph S. 
Published: December 2021

AbstractThis essay attempts to assess the theological significance of French Jesuit investment in the study of Origen. De Lubac and Daniélou read Origen as a vir ecclesiasticus, making him a resource for Vatican II’s new vision of the Church. They saw him as a scriptural thinker rather than a philosopher, and thus revealed him to be a master of spirituality. Origen’s account of the economy of salvation, founded on the Logos enlightening all minds, prompted the Jesuit theologians (heirs of such daring missionaries as Matteo Ricci and Ippolito Desideri) to undertake an interreligious thinking that was ahead of its time. While celebrating the rich syntheses thus created, which greatly broadened the Catholic mind after the crackdown against Modernism, we may find it more fruitful today to trace the significant tensions and ambiguities within the Jesuit reception and in Origen’s own thought, notably the tension between the soil of the Gospel (and of Jewish experience) and the Platonizing thought‐forms that Daniélou attacked and de Lubac cherished.
“I pretty much followed the law, and there weren't any decisions to make”: A qualitative study of self‐advocacy experiences of men with cancer
Authors: Thomas Teresa Hagan , Hadley Makenna L. , Nilsen Marci Lee 
Published: December 2021

AbstractCurrent conceptualizations of patient self‐advocacy focus on women with cancer, leaving knowledge of male self‐advocacy deficient. The purpose of this study is to describe the key components of self‐advocacy among men with cancer. Adult (≥18 years old) men with a history of invasive cancer were recruited from cancer clinics and registries. Trained researchers led individual semi‐structured interviews regarding participants' challenges, how they overcame those challenges, and barriers and facilitators to their self‐advocacy. All interviews were analyzed using descriptive content analysis methods and synthesized into major themes. These themes were refined after receiving feedback from key stakeholders. Participants (N = 28) reported three major self‐advocacy themes: (i) managing through information and planning; (ii) finding the best team and falling in line; and (iii) strategic social connections. These themes are richly described with representative quotations for each theme and subtheme. Based on these findings, existing models of patient self‐advocacy should be adjusted to encompass how men self‐advocate. Clinicians should consider how gender may impact how and why patients with cancer self‐advocate so that they can best support their patients in achieving patient‐centered care.
All you need is … a network: The rise of interpretive public administration
Authors: Bevir Mark , Rhodes R. A. W. 
Published: December 2021

AbstractFor this 100th Anniversary issue of Public Administration, we review one of the more distinctive trends in the study of public administration—the rise of the interpretive approach. We tie interpretivism to an anti‐naturalist historicism and humanism in contrast to the formalism and scientism of mainstream public administration. We review developments in Britain, Western Europe, and the United States, concluding there is a divide between the empiricist Anglo‐Saxon approaches and Continental interpretive and critical approaches. We summarize Bevir and Rhodes' decentred approach with its focus on traditions, beliefs, practices, and dilemmas. We highlight its distinctive features compared with other interpretive approaches. We suggest the interpretive approach could develop further by blurring genres and practising bricolage. We conclude that interpretivism thrives in Britain and Continental Europe because there are established networks behind which lies the nineteenth century European tradition of hermeneutics and idealism.
Globalization and Gender‐Specific Patterns in Individual Fertility Decisions
Authors: Piriu Andreea Alexandra 
Published: December 2021

AbstractIn the German post‐reunification context dominated by economic uncertainty and structural change, this paper studies the effects of import shocks from China on the fertility decisions of individuals working in the German manufacturing sector between 1995 and 2016. While focusing on trade shocks related to Chinese imported goods, the paper explores individual fertility via the labor market outcomes of manufacturing workers, roughly a fifth of German employment. I investigate the gender‐specific effects of Chinese import competition on individual fertility and explain the channels mediating each of them. I find that globalization affects overall fertility negatively, but the effect is positive for women and negative for men. Results indicate a reduction in the employment opportunity of individuals, an increase in marginal employment and higher economic insecurity. There is a substitution effect in the labor supply of women, here prevalently concentrated in low‐technology sectors: as female earnings fall and their opportunity cost of work is lower, the prospect of having children possibly becomes a more rewarding alternative. Given concerns over low fertility in Germany, findings are particularly important for understanding the German social and economic structure that enabled the country's post‐reunification transformation but also allowed heavy labor market segmentation and atypical work.
Postcrania of Borealestes (Mammaliformes, Docodonta) and the emergence of ecomorphological diversity in early mammals
Authors: Panciroli Elsa , Benson Roger B.J. , Fernandez Vincent , Humpage Matthew , Martín‐Serra Alberto , Walsh Stig , Luo Zhe‐Xi , Fraser Nicholas C. 
Published: December 2021

AbstractThe Middle Jurassic witnessed the early diversification of mammal groups, including the stem‐mammalian clade, Docodonta. Recent discoveries in China indicate docodontans exhibited ecomorphological diversity akin to small‐bodied mammals living >100 million years later, in the Cenozoic. Our understanding of the emergence of this ecological diversity is hindered by a lack of Middle Jurassic fossil material from other parts of the world. The two partial postcranial skeletons of Borealestes described here come from the Kilmaluag Formation, Scotland. These are the most complete Mesozoic mammaliaform skeletons currently known from the UK, and among the best preserved in Europe. As an early member of Docodonta, Borealestes provides key anatomical information for understanding the clade’s evolution, and the emergence of mammaliaform ecomorphological diversity. Using digital reconstructions from micro‐CT and synchrotron scans, we describe the postcranial anatomy of Borealestes and provide an updated phylogenetic analysis incorporating cranial and postcranial characters. We find Borealestes species form a sister group to a clade comprising Agilodocodon and Microdocodon. To complement observational analyses of the skeleton, we carry out principal components analyses using 3D landmarks on a comparative dataset of 42 extant mammal taxa. Our results indicate Borealestes lacked specializations for derived locomotor behaviour. We detect some similarity in the humerus between Borealestes and Ornithorhynchus. Borealestes is morphologically intermediate between the robust morphology of fossorial and semi‐fossorial/semi‐aquatic Haldanodon and Docofossor, and the gracile morphology for scansorial Agilodocodon and Microdocodon. We suggest ecological diversity in Docodonta may arise from an unspecialized basal bauplan, of which Borealestes may be representative.
β2‐microglobulin measurement with dried urine spots for congenital anomalies of the kidney and urinary tract screening in 3‐year‐old children
Authors: Morohashi Tamaki , Wada Naohiro , Odaira Sakiko , Shimizu Shoichi , Takahashi Shori , Morioka Ichiro 
Published: December 2021

AbstractBackgroundScreening using dipstick urinalysis has long been performed in 3‐year‐old children; however, it is ineffective in detecting congenital anomalies of the kidney and urinary tract (CAKUT). Measurement of the urinary β2‐microglobulin (β2MG)/creatinine (Cr) ratio may be more effective for this purpose. Analysis of dried urine spots (DUS) on filter paper is suitable for mass screening since operational costs are low and samples are easy to collect and transport. We examined the accuracy of measuring the urinary β2MG/Cr ratio in DUS on filter paper.MethodsWe collected 2,623 urine samples from 3‐year‐old children. β2MG and Cr levels were measured in DUS on filter paper. We examined the correlation between the β2MG/Cr ratios measured in DUS and using the conventional method in 640 samples using the coefficient of determination test. Children with high β2MG/Cr ratios (>0.6 µg/mg Cr) in DUS samples were further examined to establish a definitive diagnosis.ResultsThere was strong correlation between the two methods for determination of β2MG levels (r2 = 0.68; P < 0.001) and β2MG/Cr ratios (r2 = 0.69; P < 0.001). Of the 2,623 children, 38 (1.45%) had β2MG/Cr ratios >0.6. Thirty‐five children were subsequently examined, resulting in findings of renal hypodysplasia (n = 2, 0.08%), horseshoe kidney (n = 1, 0.04%), renal tubular dysfunction with hepatoblastoma (n = 1, 0.04%), data abnormality (high urine β2MG level, n = 6, 0.23%; high serum Cr level, n = 1, 0.04%), and normal values (n = 24, 0.91%).ConclusionsWe evaluated a practical method for measuring β2MG/Cr ratios in DUS as a screening method to detect CAKUT in 3‐year‐old children.
The 100 most‐cited papers in dentistry for individuals with neurodevelopmental disorders: Bibliometric profile of scientific research
Authors: Kammer Pedro Vitali , Moro Juliana Silva , Martins‐Júnior Paulo Antônio , Cardoso Mariane , Bolan Michele , Santana Carla Miranda 
Published: December 2021

AbstractAimTo conduct a bibliometric analysis of the top 100 most‐cited papers in dentistry for individuals with neurodevelopmental disorders.Methods and ResultsA search strategy was constructed and conducted at the Web of Science in the category of “Dentistry, Oral Surgery, and Medicine”. The following information was extracted from each paper: title, authorship, year of publication, title of the journal, study subject, study design, age range of sample participants, type of disability, institution, country and continent, number of citations and citation density. Google Scholar and Scopus were used to crosscheck the number of citations of the most‐cited papers. The VOSviewer software was used to generate bibliometric network maps. The papers received 4453 citations in Web of Science, ranging from 30 to 106 citations. Most papers were published by the USA (35%), had an observational design (78%) and the main subject was epidemiology (31%). Most studies focused on individuals with Down syndrome (34%) and Autism Spectrum Disorder (18%) and were published in the Journal of Periodontology (10%) and the Pediatric Dentistry Journal (7%).ConclusionMost of the studies included in this review were characterized as epidemiological studies. Future research should focus on behavior guidance, dental education, and access to dental services.
Cross‐education effects of unilateral accentuated eccentric isoinertial resistance training on lean mass and function
Authors: Maroto‐Izquierdo Sergio , Nosaka Kazunori , Blazevich Anthony J. , González‐Gallego Javier , de Paz José A. 
Published: December 2021

AbstractPurposeWe investigated the effects of three different unilateral isoinertial resistance training protocols with eccentric overload on changes in lean mass and muscle function of trained (TL) and contralateral non‐trained (NTL) legs.MethodsPhysically active university students were randomly assigned to one of three training groups or a control group (n = 10/group). Participants in the training groups performed dominant leg isoinertial squat training twice a week for 6 weeks (4 sets of 7 repetitions) using either an electric‐motor device with an eccentric phase velocity of 100% (EM100) or 150% (EM150) of concentric phase velocity or a conventional flywheel device (FW) with the same relative inertial load. Changes in thigh lean mass, unilateral leg‐press one‐repetition maximum (1‐RM), muscle power at 40–80% 1‐RM, and unilateral vertical jump height before and after training were compared between the groups and between TL and NTL.ResultsNo changes in any variable were found for the control group. In TL, all training groups showed similar increases (p < 0.05) in 1‐RM strength (22.4–30.2%), lean tissue mass (2.5–5.8%), muscle power (8.8–21.7%), and vertical jump height (9.1–32.9%). In NTL, 1‐RM strength increased 22.0–27.8% without significant differences between groups; however, increases in lean mass (p < 0.001) were observed for EM150 (3.5%) and FW (3.8%) only. Unilateral vertical jump height (6.0–32.9%) and muscle power (6.8–17.5%) also increased in NTL without significant differences between training groups.ConclusionThe three eccentric‐overload resistance training modalities produced similar neuromuscular changes in both the trained and non‐trained legs, suggesting that strong cross‐education effects were induced by the eccentric‐overload training.
The kinetochore protein NNF1 has a moonlighting role in the vegetative development of Arabidopsis thaliana
Authors: Allipra Sreejith , Anirudhan Krishnapriya , Shivanandan Siddharth , Raghunathan Abhishek , Maruthachalam Ravi 
Published: December 2021

SUMMARYThe kinetochore is a supramolecular protein complex assembled on the chromosomes, essential for faithful segregation of the genome during cell divisions. More than 100 proteins are known to constitute the eukaryotic kinetochore architecture, primarily identified using non‐plant organisms. A majority of them are fast evolving and are under positive selection. Thus, functional characterization of the plant kinetochore proteins is limited as only a few conserved orthologs sharing sequence similarity with their animal counterparts have been examined. Here, we report the functional characterization of the Arabidopsis thaliana homolog of the yeast NNF1/human PMF1 outer kinetochore protein and show that it has both kinetochore and non‐kinetochore functions in plant growth and development. Knockout of NNF1 causes embryo lethality implying its essential role in cell division. AtNNF1 interacts with MIS12 in Y2H and co‐immunoprecipitation assays, confirming it is one of the constituents of the plant MIS12 complex. GFP‐NNF1 localizes to the kinetochore, rescuing the embryo lethal nnf1‐1−/− phenotype, but the rescued plants (GFP‐NNF1nnf1−/−) are dwarf, displaying hypomorphic phenotypes with no evidence of mitotic or meiotic segregation defects. GFP‐NNF1nnf1−/− dwarf plants have reduced levels of endogenous polyamines, which are partially rescued to wild‐type levels upon exogenous application of polyamines. Mutations in the putative leucine zipper‐like binding motif of NNF1 gave rise to a dominant‐negative tall plant phenotype reminiscent of constitutive gibberellic acid (GA) action. These contrasting hypomorphic dwarf and antimorphic tall phenotypes facilitated us to attribute a moonlighting role to Arabidopsis NNF1 affecting polyamine and GA metabolism apart from its primary role in kinetochores.
MRI monitoring of transplanted neonatal porcine islets labeled with polyvinylpyrrolidone‐coated superparamagnetic iron oxide nanoparticles in a mouse model
Authors: Purich Kieran , Cai Haolei , Yang Bin , Xu Zhihao , Tessier Anthony G. , Black Adnan , Hung Ryan W. , Boivin Eric , Xu Baoyou , Wu Ping , Zhang Bo , Xin Dong , Fallone Biagio Gino , Rajotte Ray V. , Wu Yulian , Rayat Gina R. 
Published: December 2021

AbstractIslet transplantation is a potential treatment option for certain patients with type 1 diabetes; however, it still faces barriers to widespread use, including the lack of tools to monitor islet grafts post‐transplantation. This study investigates whether labeling neonatal porcine islets (NPI) with polyvinylpyrrolidone‐coated superparamagnetic iron oxide nanoparticles (PVP‐SPIO) affects their function, and whether this nanoparticle can be utilized to monitor NPI xenografts with magnetic resonance imaging (MRI) in a mouse model. In vitro, PVP‐SPIO‐labeled NPI in an agarose gel was visualized clearly by MRI. PVP‐SPIO‐labeled islets were then transplanted under the kidney capsules of immunodeficient nondiabetic and diabetic mice. All diabetic mice that received transplantation of PVP‐SPIO‐labeled islets reached normoglycemia. Grafts appeared as hypo‐intense areas on MRI and were distinguishable from the surrounding tissues. Following injection of spleen cells from immunocompetent mice, normoglycemic recipient mice became diabetic and islet grafts showed an increase in volume, accompanied by a mixed signal on MRI. Overall, this study demonstrates that PVP‐SPIO did not affect the function of NPI that PVP‐SPIO‐labeled islets were easily seen on MRI, and changes in MRI signals following rejection suggest a potential use of PVP‐SPIO‐labeled islets to monitor graft viability.