bluuebunny/crossref_metadata_2025_split huggingface dataset:
Single-Dose Messenger RNA Vaccine Effectiveness Against Severe Acute Respiratory Syndrome Coronavirus 2 in Healthcare Workers Extending 16 Weeks Postvaccination: A Test-Negative Design From Québec, Canada
Authors: Carazo Sara , Talbot Denis , Boulianne Nicole , Brisson Marc , Gilca Rodica , Deceuninck Geneviève , Brousseau Nicholas , Drolet Mélanie , Ouakki Manale , Sauvageau Chantal , Barkati Sapha , Fortin Élise , Carignan Alex , De Wals Philippe , Skowronski Danuta M , De Serres Gaston 
Published: August 2021

Abstract

Background
In Canada, first and second doses of messenger RNA (mRNA) vaccines against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) were uniquely spaced 16 weeks apart. We estimated 1- and 2-dose mRNA vaccine effectiveness (VE) among healthcare workers (HCWs) in Québec, Canada, including protection against varying outcome severity, variants of concern (VOCs), and the stability of single-dose protection up to 16 weeks postvaccination.

Methods
A test-negative design compared vaccination among SARS-CoV-2 test–positive and weekly matched (10:1), randomly sampled, test-negative HCWs using linked surveillance and immunization databases. Vaccine status was defined by 1 dose ≥14 days or 2 doses ≥7 days before illness onset or specimen collection. Adjusted VE was estimated by conditional logistic regression.

Results
Primary analysis included 5316 cases and 53 160 controls. Single-dose VE was 70% (95% confidence interval [CI], 68%–73%) against SARS-CoV-2 infection; 73% (95% CI, 71%–75%) against illness; and 97% (95% CI, 92%–99%) against hospitalization. Two-dose VE was 86% (95% CI, 81%–90%) and 93% (95% CI, 89%–95%), respectively, with no hospitalizations. VE was higher for non-VOCs than VOCs (73% Alpha) among single-dose recipients but not 2-dose recipients. Across 16 weeks, no decline in single-dose VE was observed, with appropriate stratification based upon prioritized vaccination determined by higher vs lower likelihood of direct patient contact.

Conclusions
One mRNA vaccine dose provided substantial and sustained protection to HCWs extending at least 4 months postvaccination. In circumstances of vaccine shortage, delaying the second dose may be a pertinent public health strategy.
R-estimators in GARCH models: asymptotics and applications
Authors: Liu Hang , Mukherjee Kanchan 
Published: August 2021

Summary
The quasi-maximum likelihood estimation is a commonly-used method for estimating the generalized autoregressive conditional heteroscedastic parameters. However, such estimators are sensitive to outliers and their asymptotic normality is proved under the finite fourth moment assumption on the underlying error distribution. In this paper, we propose a novel class of estimators of the generalized autoregressive conditional heteroscedastic parameters based on ranks of the residuals, called R-estimators, with the property that they are asymptotically normal under the existence of a finite $2+\delta$ moment of the errors and are highly efficient. We propose a fast algorithm for computing the R-estimators. Both real data analysis and simulations show the superior performance of the proposed estimators under the heavy-tailed and asymmetric distributions.
Investigation of the stability and risks of fomite transmission of human coronavirus OC43 on leather
Authors: Shivkumar Maitreyi , Adkin Pat , Owen Lucy , Laird Katie 
Published: August 2021

Abstract
Limited research exists on the potential for leather to act as a fomite of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) or endemic coronaviruses including human coronavirus (HCoV) OC43; this is important for settings such as the shoe manufacturing industry. Antiviral coating of leather hides could limit such risks. This study aimed to investigate the stability and transfer of HCoVOC43 on different leathers, as a surrogate for SARS-CoV-2, and assess the antiviral efficacy of a silver-based leather coating. The stability of HCoV-OC43 (6.6 log10) on patent, full-grain calf, corrected grain finished and nubuck leathers (silver additive-coated and uncoated) was measured by titration on BHK-21 cells. Transfer from leather to cardboard and stainless steel was determined. HCoV-OC43 was detectable for 6 h on patent, 24 h on finished leather and 48 h on calf leather; no infectious virus was recovered from nubuck. HCoV-OC43 transferred from patent, finished and calf leathers onto cardboard and stainless steel up to 2 h post-inoculation (≤3.1–5.5 log10), suggesting that leathers could act as fomites. Silver additive-coated calf and finished leathers were antiviral against HCoV-OC43, with no infectious virus recovered after 2 h and limited transfer to other surfaces. The silver additive could reduce potential indirect transmission of HCoV-OC43 from leather.
Simple-to-use CRISPR-SpCas9/SaCas9/AsCas12a vector series for genome editing in Saccharomyces cerevisiae
Authors: Okada Satoshi , Doi Goro , Nakagawa Shitomi , Kusumoto Emiko , Ito Takashi 
Published: August 2021

Abstract
Genome editing using the CRISPR/Cas system has been implemented for various organisms and becomes increasingly popular even in the genetically tractable budding yeast Saccharomyces cerevisiae. Because each CRISPR/Cas system recognizes only the sequences flanked by its unique protospacer adjacent motif (PAM), a certain single system often fails to target a region of interest due to the lack of PAM, thus necessitating the use of another system with a different PAM. Three CRISPR/Cas systems with distinct PAMs, namely SpCas9, SaCas9, and AsCas12a, have been successfully used in yeast genome editing. Their combined use should expand the repertoire of editable targets. However, currently available plasmids for these systems were individually developed under different design principles, thus hampering their seamless use in the practice of genome editing. Here, we report a series of Golden Gate Assembly-compatible backbone vectors designed under a unified principle to exploit the three CRISPR/Cas systems in yeast genome editing. We also created a program to assist the design of genome-editing plasmids for individual target sequences using the backbone vectors. Genome editing with these plasmids demonstrated practically sufficient efficiency in the insertion of gene fragments to essential genes (median 52.1%), the complete deletion of an open reading frame (median 78.9%), and the introduction of single amino acid substitutions (median 79.2%). The backbone vectors with the program would provide a versatile toolbox to facilitate the seamless use of SpCas9, SaCas9, and AsCas12a in various types of genome manipulation, especially those that are difficult to perform with conventional techniques in yeast genetics.
Longitudinal Relation Between Structural Network Efficiency, Cognition, and Gait in Cerebral Small Vessel Disease
Authors: Cai Mengfei , Jacob Mina A , Norris David G , de Leeuw Frank-Erik , Tuladhar Anil M 
Published: August 2021

Abstract

Background
To investigate changes in gait performance over time and how these changes are associated with the decline in structural network efficiency and cognition in older patients with cerebral small vessel disease (SVD).

Methods
In a prospective, single-center cohort with 217 older participants with SVD, we performed 1.5T MRI scans, cognitive tests, and gait assessments evaluated by Timed UP and Go (TUG) test twice over 4 years. We reconstructed the white matter network for each subject based on diffusion tensor imaging tractography, followed by graph-theoretical analyses to compute the global efficiency. Conventional MRI markers for SVD, that is, white matter hyperintensity (WMH) volume, number of lacunes, and microbleeds, were assessed.

Results
Baseline global efficiency was not related to changes in gait performance, while decline in global efficiency over time was significantly associated with gait decline (ie, increase in TUG time), independent of conventional MRI markers for SVD. Neither baseline cognitive performance nor cognitive decline was associated with gait decline.

Conclusions
We found that disruption of the white matter structural network was associated with gait decline over time, while the effect of cognitive decline was not. This suggests that structural network disruption has an important role in explaining the pathophysiology of gait decline in older patients with SVD, independent of cognitive decline.
A Dilute-and-Shoot UHPLC–MS/MS Isotope Dilution Method for Simultaneous Determination and Confirmation of 11 Mycotoxins in Dried Distiller’s Grains with Solubles
Authors: Nochetto Cristina B , Li Hui 
Published: August 2021

Abstract

Background
Natural contamination with mycotoxins in dried distiller’s grains with solubles (DDGS) as a mainstream animal feed ingredient poses a risk to animal health.

Objective
A regulatory method was needed for the agency to simultaneously detect 11 mycotoxins of high regulatory priority in DDGS.

Methods
A DDGS sample (10 g) was extracted twice with acetonitrile-water under mildly acidic condition. Two aliquots from the combined crude extract were taken and processed separately: (1) diluted 400-fold with solvent for analysis of deoxynivalenol and fumonisins B1 and B2; and (2) with the pH adjusted to 7.5, and then diluted 15.7-fold for analysis of aflatoxins B1, B2, G1, and G2, ochratoxin A, zearalenone, and T-2 and HT-2 toxins. Uniformly labeled 13C-isotopologues of these mycotoxins were added as internal standards to the diluted extracts for quantitative analysis by ultra-high-performance LC–tandem MS.

Results
The linear quantitation ranges (µg/kg) were: aflatoxins B1, B2, G1, and G2, 1.57–105; zearalenone, 16.3–1090; T-2 toxin, 3.14–208; HT-2 toxin, 48.2–3220; ochratoxin A, 0.47–31.4; deoxynivalenol, 240–16 000; fumonisin B1 and B2, 320–21 200. Accuracies for these analytes at each of three fortification levels ranged from 70.7 to 100%, with corresponding RSDs between 1.4 and 10.5%. True recoveries were all higher than 83%.

Conclusion
This method was successfully validated to meet the agency’s performance guidelines for regulatory methods.

Highlights
This method is easy, quick, and robust to simultaneously quantify and confirm the presence of 11 regulated mycotoxins in DDGS.
Ad-pinitol transporter, LjPLT11, regulates plant growth and nodule development inLotus japonicus
Authors: Tian Lu , Liu Leru , Xu Shaoming , Deng Rufang , Wu Pingzhi , Jiang Huawu , Wu Guojiang , Chen Yaping 
Published: August 2021

AbstractPolyol transporters have been functionally characterized in yeast and Xenopus laevis oocytes as H+-symporters with broad substrate specificity, but little is known about their physiological roles in planta. To extend this knowledge, we investigated the role of LjPLT11 in Lotus japonicus–Mesorhizobium symbiosis. Functional analyses of LjPLT11 in yeast characterized it as an energy-independent transporter of xylitol, two O-methyl inositols, xylose, and galactose. We showed that LjPLT11 is located on peribacteroid membranes and functions as a facilitative transporter of d-pinitol within infected cells of L. japonicus nodules. Knock-down of LjPLT11 (LjPLT11i) in L. japonicus accelerated plant growth under nitrogen sufficiency, but resulted in abnormal bacteroids with corresponding reductions in nitrogenase activity in nodules and plant growth in the nitrogen-fixing symbiosis. LjPLT11i nodules had higher osmotic pressure in cytosol, and lower osmotic pressure in bacteroids, than wild-type nodules both 3 and 4 weeks after inoculation of Mesorhizobium loti. Levels and distributions of reactive oxygen species were also perturbed in infected cells of 4-week-old nodules in LjPLT11i plants. The results indicate that LjPLT11 plays a key role in adjustment of the levels of its substrate pinitol, and thus maintenance of osmotic balance in infected cells and peribacteroid membrane stability during nodule development.
Three-dimensional quantitative analysis of the Arabidopsis quiescent centre
Authors: Lu Ran , Canher Balkan , Bisht Anchal , Heyman Jefri , De Veylder Lieven 
Published: August 2021

AbstractQuiescent centre (QC) cells represent an integral part of the root stem cell niche. They typically display a low division frequency that has been reported to be controlled by hormone signaling and different regulators, including the ETHYLENE RESPONSE FACTOR 115 (ERF115) transcription factor and D-type cyclins. Here, we applied a three-dimensional (3D) imaging to visualize the Arabidopsis QC cell number, volume and division patterns, including visualization of anticlinal divisions that cannot be deduced from longitudinal 2D imaging. We found that 5-day-old seedlings possess on average eight QC cells which are organized in a monolayered disc. In a period of 7 d, half of the QC cells undergo anticlinal division in a largely invariant space. Ectopic expression of ERF115 and CYCLIN D1;1 (CYCD1;1) promote both anticlinal and periclinal QC cell divisions, the latter resulting in a dual-layered QC zone holding up to 2-fold more QC cells compared with the wild type. In contrast, application of cytokinin or ethylene results in an increase in the number of periclinal, but a decrease in anticlinal QC divisions, suggesting that they control the orientation of QC cell division. Our data illustrate the power of 3D visualization in revealing unexpected QC characteristics.
Intoxicating Blackness: Addiction and Ambivalent Sounds of Fugitive Life in James Baldwin’s “Sonny’s Blues”
Authors: Walter Patrick F 
Published: August 2021

Abstract
This article examines the intersection of drug-use, aesthetics, and Blackness at work in James Baldwin’s short story “Sonny’s Blues” (1957). While much of the scholarship on Baldwin’s story has dwelt on the significance of music, far less attention has been paid to the equally prominent and related theme of drug addiction and intoxication. Through an historical contextualization and analysis of the story, I provide an exegesis of Baldwin’s unique theorization of drug addiction, or what I am calling intoxicating Blackness, particularly as it relates to black aesthetics. By surveying formulations of drug addiction in European thought and the counter-discourse of black radical thought, I argue that Baldwin’s short story figures an innovative notion of being on drugs. Unlike subsequent, often disparaging depictions of addiction in the political discourse of the Black Power Movement and other late twentieth-century black popular culture, ‘Sonny’s Blues’ represents addiction as a cite of possibility. Baldwin suggests that an openness to intoxication and addiction, although perilous for the addict, might function as an aesthetic of black fugitivity and that any sort of black life might be thought of as a dangerous, fugitive narcotic. To elaborate on this theory of intoxicating Blackness, I consider it in relation to Western philosophical, legal, and psychoanalytic paradigms of addiction, as well as conceptualizations of Blackness and fugitivity in the work of thinkers such as Sylvia Wynter, Fred Moten, and Frantz Fanon.
What Do People Want in a Smoking Cessation App? An Analysis of User Reviews and App Quality
Authors: Bendotti Hollie , Lawler Sheleigh , Ireland David , Gartner Coral , Hides Leanne , Marshall Henry M 
Published: August 2021

AbstractIntroductionMobile smoking cessation (mCessation) apps have the potential to complement and enhance existing interventions, but many are of low quality. Exploring app reviews can provide a broader understanding of user experiences and engagement, to enhance the quality, acceptability, and effectiveness of future developments.MethodsPublicly available user reviews and ratings of smoking cessation apps were mined from Google Play and the App Store via a targeted two-stage search strategy. English language smoking cessation apps with at least 20 consumer reviews between 2011 and 2020 were included. User reviews were thematically analyzed using Braun and Clarke’s framework. Apps were independently scored using the Mobile Apps Rating Scale (MARS) and compared to average user star ratings.ResultsForty-eight versions of 42 apps, encompassing 1414 associated reviews, met eligibility criteria. Inductive coding of reviews produced 1084 coding references including reviews coded across multiple nodes. Themes generated included: (1) supportive characteristics/tools; (2) useability; (3) influence on smoking behavior; (4) benefits of quitting; and (5) role as a supplementary tool for quitting. The mean MARS score of 36 free and accessible apps was 3.10 (SD 0.71) with mean scores ranging from 2.00 to 4.47. An inverse relationship between MARS scores and average user star ratings was observed.ConclusionsApp personalization, relationality, functionality, and credibility were important to users, and should be considered as key design components for future apps. Differences between user star ratings and MARS scores may illustrate competing priorities of consumers and researchers, and the importance of a codesign development method.ImplicationsThis is the first study to use unsolicited user reviews from a large population to understand the general mCessation user experience in relation to making a quit attempt. Our findings highlight specific features favored and disliked by users, including their influence on engagement, and supports previous findings that mCessation applications need to be highly tailorable, functional, credible, and supportive. We recommend a consumer-driven, co-design approach for future mCessation app developments to optimize user acceptability and engagement.
Vancomycin or Daptomycin for Outpatient Parenteral Antibiotic Therapy: Does It Make a Difference in Patient Satisfaction?
Authors: Wu Katherine H , Sakoulas George , Geriak Matthew 
Published: August 2021

Abstract
A 5-question telephone survey was administered to compare satisfaction between patients receiving vancomycin vs daptomycin outpatient parenteral antimicrobial therapy (OPAT). Twenty-seven patients completed the survey (40%). Vancomycin had higher daily interference score than daptomycin (P = .03). All patients receiving daptomycin reported a satisfaction score ≥8/10, as compared to 67% of patients who received vancomycin (P &lt; .03). OPAT antibiotics with less cumbersome administration regimens may translate into higher patient satisfaction and quicker return to life normalcy.
The Physcomitrium (Physcomitrella) patens PpKAI2L receptors for strigolactones and related compounds function via MAX2-dependent and -independent pathways
Authors: Lopez-Obando Mauricio , Guillory Ambre , Boyer François-Didier , Cornu David , Hoffmann Beate , Le Bris Philippe , Pouvreau Jean-Bernard , Delavault Philippe , Rameau Catherine , de Saint Germain Alexandre , Bonhomme Sandrine 
Published: August 2021

Abstract
In angiosperms, the α/β hydrolase DWARF14 (D14), along with the F-box protein MORE AXILLARY GROWTH2 (MAX2), perceives strigolactones (SL) to regulate developmental processes. The key SL biosynthetic enzyme CAROTENOID CLEAVAGE DIOXYGENASE8 (CCD8) is present in the moss Physcomitrium patens, and PpCCD8-derived compounds regulate moss extension. The PpMAX2 homolog is not involved in the SL response, but 13 PpKAI2LIKE (PpKAI2L) genes homologous to the D14 ancestral paralog KARRIKIN INSENSITIVE2 (KAI2) encode candidate SL receptors. In Arabidopsis thaliana, AtKAI2 perceives karrikins and the elusive endogenous KAI2-Ligand (KL). Here, germination assays of the parasitic plant Phelipanche ramosa suggested that PpCCD8-derived compounds are likely noncanonical SLs. (+)-GR24 SL analog is a good mimic for PpCCD8-derived compounds in P. patens, while the effects of its enantiomer (−)-GR24, a KL mimic in angiosperms, are minimal. Interaction and binding assays of seven PpKAI2L proteins pointed to the stereoselectivity toward (−)-GR24 for a single clade of PpKAI2L (eu-KAI2). Enzyme assays highlighted the peculiar behavior of PpKAI2L-H. Phenotypic characterization of Ppkai2l mutants showed that eu-KAI2 genes are not involved in the perception of PpCCD8-derived compounds but act in a PpMAX2-dependent pathway. In contrast, mutations in PpKAI2L-G, and -J genes abolished the response to the (+)-GR24 enantiomer, suggesting that PpKAI2L-G, and -J proteins are receptors for moss SLs.
A digital sensor to measure real-time leaf movements and detect abiotic stress in plants
Authors: Geldhof Batist , Pattyn Jolien , Eyland David , Carpentier Sebastien , Van de Poel Bram 
Published: August 2021

Abstract
Plant and plant organ movements are the result of a complex integration of endogenous growth and developmental responses, partially controlled by the circadian clock, and external environmental cues. Monitoring of plant motion is typically done by image-based phenotyping techniques with the aid of computer vision algorithms. Here we present a method to measure leaf movements using a digital inertial measurement unit (IMU) sensor. The lightweight sensor is easily attachable to a leaf or plant organ and records angular traits in real-time for two dimensions (pitch and roll) with high resolution (measured sensor oscillations of 0.36 ± 0.53° for pitch and 0.50 ± 0.65° for roll). We were able to record simple movements such as petiole bending, as well as complex lamina motions, in several crops, ranging from tomato to banana. We also assessed growth responses in terms of lettuce rosette expansion and maize seedling stem movements. The IMU sensors are capable of detecting small changes of nutations (i.e. bending movements) in leaves of different ages and in different plant species. In addition, the sensor system can also monitor stress-induced leaf movements. We observed that unfavorable environmental conditions evoke certain leaf movements, such as drastic epinastic responses, as well as subtle fading of the amplitude of nutations. In summary, the presented digital sensor system enables continuous detection of a variety of leaf motions with high precision, and is a low-cost tool in the field of plant phenotyping, with potential applications in early stress detection.
Ambulatory surgery for cancer patients: current controversies and concerns
Authors: Guerra-Londono Carlos E. , Kim Daniel , Ramirez Manotas Maria F. 
Published: August 2021

Purpose of review
This review aims to describe the main concerns and controversies of ambulatory surgery in cancer patients while providing an overview of ambulatory cancer anaesthesia.

Recent findings
Cancer patients can undergo a variety of ambulatory surgeries. The introduction of robotic approach and the implementation of enhanced recovery programmes have allowed patients to avoid hospital admissions after more complex or invasive surgeries. In this context, the anaesthesiologist plays a key role in ensuring that the ambulatory surgical centre or the hospital-based ambulatory department is equipped for the perioperative challenges of the cancer population. Cancer patients tend to be older and with more comorbidities than the general population. In addition, these individuals may suffer from chronic conditions solely because of the cancer itself, or the treatment. Consequently, frailty is not uncommon and should be screened on a routine basis. Regional analgesia plays a key role in the provision of opioid-sparing multimodal analgesia.

Summary
Neither regional anaesthesia or general anaesthesia have proven to affect the long-term oncological outcomes of cancer patients undergoing ambulatory surgery. In addition, there is insufficient evidence to suggest the use of total intravenous anaesthesia or inhalational anaesthesia over the other to decrease cancer recurrence.