bluuebunny/crossref_metadata_2025_split huggingface dataset:
Noise Reduction for Radio Map Crowdsourcing Building in WLAN Indoor Localization System
Authors: zhang liye , Wang Zhuang , Meng Xiaoliang , Fang Chao , Liu Cong 
Published: May 2021

Abstract
Recent years have witnessed a growing interest in using WLAN fingerprint-based method for indoor localization system because of its cost effectiveness and availability compared to other localization systems. In order to rapidly deploy WLAN indoor positioning system, the crowdsourcing method is applied to alternate the traditional deployment method. In this paper, we proposed a fast radio map building method utilizing the sensors inside the mobile device and the Multidimensional Scaling (MDS) method. The crowdsourcing method collects RSS and sensor data while the user is walking along a straight line and computes the position information using the sensor data. In order to reduces the noise in the location space of the radio map, the Short Term Fourier Transform (STFT) method is used to detect the usage mode switching to improve the step determination accuracy. When building a radio map, much fewer RSS values are needed using the crowdsourcing method compared to conventional methods, which lends greater influence to noises and erroneous measurements in RSS values. Accordingly, an imprecise radio map is built based on these imprecise RSS values. In order to acquire a smoother radio map and improve the localization accuracy, the MDS method is used to infer an optimal RSS value at each location by exploiting the correlation of RSS values at nearby locations. Experimental results show that the expected goal is achieved by the proposed method.
Analysis of Pre-Erythrocytic Immunity During Plasmodium Vivax Infection Reveals a Diversity of Responses That is Partially Due to Blood Stage Cross-Reactivity
Authors: Ventocilla Julio , Tapia L. Lorena , Sperling Lisa , Ponce Reynaldo , Franco Adriano , Leelawong Mindy , Aguiar Joao C. , Baldeviano G. Christian , Wilder Brandon K. 
Published: May 2021

Abstract
BackgroundPlasmodium vivax (Pv) represents the most geographically widespread human malaria parasite. Targeting the pre-erythrocytic (PE) stage of the parasite life cycle is especially appealing for Pv vaccines as it would prevent disease and transmission. Here, we explore naturally acquired immunity to a panel of Pv PE antigens as a first step to enable vaccine development and to better understand naturally-acquired PE immunity.MethodsHumoral and cellular immunity were evaluated by ELISA and ELISpot, using samples from Pv infected individuals from a low endemic malaria region in the Peruvian Amazon Basin. In addition, we utilized experimental infection of Aotus non-human primates with Pv or P. falciparum (Pf) in order to evaluate the contribution of blood stage infection to the humoral response observed in human samples.ResultsIn our clinical samples, twelve PE antigens showed positive antibody reactivity with variable prevalence of 58–99%. The magnitude of the IgG antibody response against PE antigens was lower compared with blood stage antigens MSP1 and DBP-II although titers persisted better for PE antigens, six months later after infection (average decrease of 6% for PE antigens and 43% for MSP1) in general. A significant correlation between IgG antibodies and number of previous malaria episodes was observed only for blood stage antigens. High IgG responders across PE and blood stage antigens showed a significantly lower parasitemia compared to low responders (median 1873 vs 4663 par/µl). We observed a positive T cell response in 35% vs 9–35% of total volunteers against blood stage antigen MSP1 and PE antigens, respectively, and saw no correlation with IgG responses. Aotus monkeys infected with Pv blood stage showed positive reactivity against the seven PE antigens tested. In contrast, only 2 of 10 monkeys infected with Pf showed low positive IgG cross-reactivity against Pv MSP1 and none of which cross-reacted to Pv CSP.ConclusionsOur results demonstrate clear humoral and T cell responses against Pv PE antigens in individuals naturally infected with P. vivax. In addition, these results are largely replicated in a novel Aotus nancymaae Pv blood stage challenge model which suggest a contribution from blood stages to PE cross-reactivity. Together, these data clearly identify novel attractive PE antigens suitable for use in the development of new malaria vaccine candidates.
Hand and Wrist Injuries Among Collegiate Athletes Vary with Athlete Division
Authors: Holoyda Kathleen , Donato Daniel , Magno-Padron David , Simpson Andrew , Agarwal Jayant 
Published: May 2021

Abstract
Background: The rates, severity and consequences of hand and wrist injuries sustained by NCAA athletes are not well characterized. This study describes the epidemiology of hand and wrist injuries among collegiate athletes competing in different divisions. Methods: The National Collegiate Athletic Association Injury Surveillance Program (NCAA-ISP) was accessed for various sports from 2004 to 2015. Data was stratified by hand and wrist injuries sustained, mean loss of activity time following the injury, male and female sport, need for surgery following injury and division. Descriptive statistics were performed to examine the association between sports, event type and gender. P < 0.05 was considered significantResults 103,098 hand and wrist injuries were reported in all NCAA sports from 2004-2015. Male athletes sustained 72,423 injuries (6.01/10,000 athlete events) and female athletes sustained 30,675 injuries (4.13/10,000 athlete events). Division I athletes sustained significantly more injuries compared to divisions II and III. Overall, 3.78% of hand and wrist injuries required surgical intervention. A significantly higher percentage of division I athletes (both male and female) underwent surgical intervention compared to divisions II and III. The mean time lost due to hand and wrist injury was 7.14 days for all athletes. Division I athletes tended to miss fewer days due to injury, though this was not significant.Conclusions Hand and wrist injuries are common among collegiate athletes. Division I athletes sustain higher rates of injuries and higher surgical intervention rates, while tending to miss fewer days due to injury. Improved characterization of divisional differences in hand and wrist injuries can assist injury management and prevention
The Dose-dependent Dual Effects of Corticosterone on Spatial Memory Ability After Traumatic Brain Injury Are Mediated by Two Corticosteroid Receptor Systems
Authors: Zhang Bin , Yang Mengshi , Yan Qiongyu , Xu Xiaojian , Niu Fei , Dong Jinqian , Zhuang Yuan , Lu Shenghua , Ge Qianqian , Liu Baiyun 
Published: May 2021

Abstract
Background: Our recent studies reported the opposite effects of mineralocorticoid receptor (MR) and glucocorticoid receptor (GR) on neuron survival after traumatic brain injury (TBI). However, as a mixed agonist for MR and GR, whether short term use of high-dose endogenous glucocorticoids exerts neurotoxic effects by excessive activation of GR, what is the set-point, and the possible signaling pathways remain unclear. This study examined the dose-dependent dual effects of corticosterone (CORT) on the spatial memory, the survival of hippocampal neurons and the possible receptor-mediated downstream signaling pathways after TBI.Methods: Based on controlled cortical impact (CCI) and CORT treatments, Sprague-Dawley rats (n=168) were randomly divided into the sham, CCI, CCI + CORT1 (0.3 mg/kg), CCI + CORT2 (3 mg/kg), CCI + CORT3 (30 mg/kg), CCI + CORT1 + spirolactone (spirolactone: 50 mg/kg/d), and CCI + CORT3 + RU486 (RU486: 50 mg/kg/d) groups. Brain tissues were collected on postinjury day 3 and processed for histology and western blot analysis.Results: On postinjury day 3, we tested the learning and memory ability, neuronal apoptosis in the hippocampus, activation levels of MR and GR, Bcl-2 family proteins, and apoptosis-related intracellular signaling pathways. We found that different doses of CORT exhibited dual effects on the survival of hippocampal neurons and the spatial memory. Lower doses of CORT (0.3, 3 mg/kg) significantly increased the activation of MR, upregulated the phosphorylation of Akt/CREB/Bad and the Bcl-2 expression, reduced the number of apoptotic neurons, and subsequently improved the spatial memory. In contrast, higher dose of CORT (30 mg/kg) exerted opposite effect by over activating GR, upregulating the expressions of P53/Bax, and inhibiting the Erk/CREB activities. Conclusion: The results suggest that there is a threshold between the neuroprotective and neurotoxic effects of endogenous GC, higher dose of which, even for short-term use, should also be avoided after TBI.
Layer-Wise Relevance Propagation for Interpreting LSTM-RNN Decisions in Predictive Maintenance
Authors: Wu Haiyue , Huang Aihua , Sutherland John W. 
Published: May 2021

Abstract
Predictive maintenance (PdM) is an advanced technique to predict the time to failure (TTF) of a system. PdM collects sensor data on the health of a system, processes the information using data analytics, and then establishes data-driven models that can forecast system failure. Deep neural networks are increasingly being used as these data-driven models owing to their high predictive accuracy and efficiency. However, deep neural networks are often criticized as being “black boxes,” which owing to their multi-layered and non-linear structure provide little insight into the underlying physics of the system being monitored, and that are nontransparent and untraceable in their predictions. In order to address this issue, the layer-wise relevance propagation (LRP) technique is applied to analyze a long short-term memory (LSTM) recurrent neural network (RNN) model. The proposed method is demonstrated and validated for a bearing health monitoring study based on vibration data. The obtained LRP results provide insights into how the model “learns” from the input data and demonstrate the distribution of contribution/relevance to the neural network classification in the input space. In addition, comparisons are made with gradient-based sensitivity analysis to show the power of LRP in interpreting RNN models. The LRP is proved to have promising potential in interpreting deep neural network models and improving model accuracy and efficiency for PdM.
Online Abortion Empowerment in Absence of Parental and Medical Support: A Thematic Analysis of a Reddit Community’s Contributions to Decision-Making and Access
Authors: Richards Nicole K , Masud Ayesha , Arocha Jose F 
Published: May 2021

Abstract
BackgroundFamily planning decisions can be influenced by several factors, including social and clinical support. Lack of support can hinder autonomous and informed pregnancy choices and healthcare access. Adolescents commonly use the internet as a communication platform and information source. This study sought to identify online reports of parental paternalism’s influence abortion knowledge and access, and how virtual a community responded to paternalistic determinants.MethodsWe explored abortion discussions on Reddit, an online platform that houses virtual communities of anonymous users (Dec 13, 2019 – Mar 1, 2020). We identified relevant posts within the “r/abortion” community using scoping review data collection methods. Relevant posts included those that discussed parents, familial/cultural values, and young age (n=93); Of these, 58 posts fit the research question. We analyzed initial posts and responses using theory-driven thematic analysis through collaborative deductive codes and thematic maps.ResultsR/abortion users discussed the parental and clinical role in family planning. Unsupportive interactions hindered users’ ability to make autonomous abortion decisions. Parents and the health system commonly upheld physical, financial, and privacy barriers. Users shared experiences and advice to overcome paternalistic influence, and offered decision-making, coping, and access resources. Young people felt empowered and informed by online conversations.ConclusionsPaternalistic barriers brought individuals to participate in r/abortion conversations. In addition to lacking knowledge to make abortion decisions, users were not informed of available services and how to navigate healthcare. R/abortion’s anonymity facilitated candid abortion discussions, provided health information, and helped users access abortion care. Peer-to-peer abortion discussions can be especially useful during COVID-19 to foster support in overcoming potentially increased isolation, parental involvement, and healthcare barriers. Family planning experts can use these communities as a tool to engage in person-centered efforts outside of traditional health systems.
Incremental Method of Generating Decision Implication Canonical Basis
Authors: Zhang Shaoxia , Li Deyu , Zhai Yanhui 
Published: May 2021

Abstract
Decision implication is an elementary representation of decision knowledge in formal concept analysis. Decision implication canonical basis (DICB), a set of decision implications with completeness and nonredundancy, is the most compact representation of decision implications. The method based on true premises (MBTP) for DICB generation is the most efficient one at present. In practical applications, however, data is always changing dynamically, and MBTP has to re-generate inefficiently the whole DICB. This paper proposes an incremental algorithm for DICB generation, which obtains a new DICB just by modifying and updating the existing one. Experimental results verify that when the samples in data are much more than condition attributes, which is actually a general case in practical applications, the incremental algorithm is significantly superior to MBTP. Furthermore, we conclude that, even for the data in which samples is less than condition attributes, when new samples are continually added into data, the incremental algorithm must be also more efficient than MBTP, because the incremental algorithm just needs to modify the existing DICB, which is only a part of work of MBTP.
Discordance of PIK3CA and TP53 Mutations Between Breast Cancer Brain Metastases and Matched Primary Tumors
Authors: THULIN ANNA , Andersson Carola , Rönnerman Elisabeth , De Lara Shahin , Chamalidou Chaido , Schoenfeldt Arnd , Kovács Anikó , Fagman Henrik , Enlund Fredrik , Linderholm Barbro K. 
Published: May 2021

Abstract
Purpose: Extensive data of mutations in breast cancer (BC) metastases has been published in recent years. However, these studies contain very few patients with brain metastasis (BM). Thus, there is limited knowledge of the biology of BCBM. We primarily aimed to compare the mutational and histological pattern of BM with matched primary breast cancer (BC). Secondary aims were to determine mutations in BMs and PT in each BC subgroup (Luminal, HER2+ and TNBC) and survival according to changed or stable mutations between PTs and BMs. Patients and methods: We investigated 57 BCBMs including 46 cases with the matched primary tumors (PT) by targeted Next Generation Sequencing (NGS) using the Cancer Hotspot Panel v2 (ThermoFisher Scientific) covering 207 targeted regions in 50 cancer related genes. BC subtype according to immunohistochemistry (estrogen- and progesterone receptors, HER2 and Ki67) was obtained by re-evaluation of available tissue from BMs and PT. Results: NGS results fulfilling sequencing quality criteria were obtained from 52 BM (91.2%) and 41 (89.1%) PT, out of which 37 were matched pairs. Pathogenic mutation was detected in 66% of PTs (27/41), and 62% of BMs (32/52). TP53 mutations were most frequent; 49% (20/41) of PTs and 48% (25/52) in BMs, followed by PIK3CA mutations; 22% (9/42) in PTs and 25% (13/52) in BMs. Mutations in CDH1, EGFR, HRAS, RB1 CDKN2A and PTEN were detected in single pairs or single samples. Mutational pattern was discordant in 24% of matched pairs. Standard BC markers was discordant in 26%, with a loss of the estrogen receptor and a change from Luminal A to other subtypes as the most common. Changes of mutations in BMs compared with PT did not influence survival after diagnose of BM (p=0.4395).Conclusions: We show a discordance of PIK3CA and TP53 mutations, as well as IHC BC subgroups in 25% of the matched pairs of BM and PT, which confirms the need for re-evaluation of mutations, as well as standard BC markers by immunohistochemistry in the BM. Since there are difficulties in obtaining tissue from BM, analysis of cell-free DNA from cerebrospinal fluid (CSF) may be a way forward.
Chryseobacterium paridis sp. nov., an endophytic bacterial species isolated from the root of Paris polyphylla Smith var. yunnanensis
Authors: Zhang Zhen , Yang Ling-Ling , Li Cong-Jian , Jiang Xing-Wang , Zhi Xiao-Yang 
Published: May 2021

Abstract
A Gram-negative, yellow-pigmented, rod-shaped bacterial strain YIM B02567 T was isolated from the root of Paris polyphylla Smith var. yunnanensis in China. Strain YIM B02567 T grew optimally at 25–30°C and at pH 7.0 in the absence of NaCl on nutrient agar. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain YIM B02567 T belong to the genus Chryseobacterium , and closely related to Chryseobacterium piperi CTM T and Chryseobacterium soli DSM 19298 T . Whole genome sequencing indicated that the genome size was 4,774,612 bp and had a DNA G+C content of 34.5 %. Values of the ANI and the dDDH between strain YIM B02567 T and its closely related Chryseobacterium species were below 81.72 % and 24.7 %. Strain YIM B02567 T contained menaquinone-6 as the sole isoprenoid quinone, anteiso-C 15:0, iso-C 17:1 ω 9 c and iso-C 17:0 3-OH as major fatty acids and phosphatidylethanolamine as major polar lipid. Based on the polyphasic analyses, strain YIM B02567 T could be differentiated genotypically and phenotypically from recognized species of the genus Chryseobacterium . The isolate therefore represents a novel species, for which the name Chryseobacterium paridis sp. nov. is proposed. The type strain is YIM B02567 T (=CGMCC 1.18657 T =JCM 5426 T ).
Dysregulated splicing factor SF3B1 unveils a dual therapeutic vulnerability to target pancreatic cancer cells and cancer stem cells with an anti-splicing drug
Authors: Alors-Perez Emilia , Blázquez-Encinas Ricardo , Alcalá Sonia , Viyuela-García Cristina , Pedraza-Arevalo Sergio , Herrero-Aguayo Vicente , Jiménez-Vacas Juan M , Mafficini Andrea , Sánchez-Frías Marina E , Cano María T , Abollo-Jiménez Fernando , Marín-Sanz Juan A , Cabezas-Sainz Pablo , Lawlor Rita T , Luchini Claudio , Sánchez Laura , Sánchez-Hidalgo Juan M , Ventura Sebastián , Martin-Hijano Laura , Gahete Manuel D , Scarpa Aldo , Arjona-Sanchez Alvaro , Ibáñez-Costa Alejandro , Sainz Bruno , Luque Raul M , Castano Justo P 
Published: May 2021

Abstract
Background
Pancreatic ductal adenocarcinoma (PDAC) remains an appallingly lethal cancer, requiring novel treatments to target both cancer cells and cancer stem cells (CSCs). Altered splicing is emerging as a novel cancer hallmark and attractive therapeutic target. The core splicing factor SF3B1 is heavily altered in cancer and can be inhibited by Pladienolide-B, but its actionability in PDAC is poorly known. We explore the presence and role of SF3B1 in PDAC and interrogate its potential as an actionable target.
Methods
SF3B1 was analyzed in PDAC tissues, a RNA-seq dataset, and public databases, examining associations with splicing alterations and key features/genes. Functional assays in PDAC cell lines and PDX-derived CSCs served to test Pladienolide-B treatment effects in vitro, and in vivo in zebrafish and mice.
Results
SF3B1 was overexpressed in human PDAC and associated with tumor grade and lymph-node involvement. SF3B1 levels closely associated with distinct splicing event profiles and expression of key PDAC players (KRAS, TP53). In PDAC cells, Pladienolide-B increased apoptosis and decreased multiple tumor-related features, including cell proliferation, migration, and colony/sphere formation, altering AKT and JNK signaling, and favoring proapoptotic splicing variants (BCL-XS/BCL-XL, KRASa/KRAS, Δ133TP53/TP53). Importantly, Pladienolide-B similarly impaired CSCs, reducing their stemness capacity and increasing their sensitivity to chemotherapy. Pladienolide-B also reduced PDAC/CSCs xenograft tumor growth in vivo in zebrafish and/or mice.
Conclusion
SF3B1 overexpression is a therapeutic vulnerability in PDAC, as altered splicing can be targeted with Pladienolide-B both in cancer cells and CSCs, paving the way for novel therapies for this lethal cancer.
The Role of Growth Hormone and IGF-1 in Retinopathy: A Prospective Study of Retinopathy in Patients with Acromegaly and Impaired Fasting Glucose
Authors: Wu Tzu-En , Chen Harn-Shen 
Published: May 2021

Abstract
We hypothesized that elevated insulin-like growth factor 1 (IGF-1) could accelerate the progress of non-proliferative retinopathy into proliferative retinopathy. We recruited 91 patients with acromegaly and 123 subjects with impaired fasting glucose (IFG) between 2008 and 2016 to examine the incidence and prevalence of retinopathy. Patients attended follow-ups in our clinics and underwent examinations according to the national guidelines for diabetes management. Both cohorts attended follow-ups until June 2019. Both groups had similar HbA1c, cholesterol, and blood pressure levels. However, patients with acromegaly had higher GH (8.05±16.18 vs. 0.78±1.25 ng/mL) and IGF-1 (547.0±342.1 vs. 146.7±51.4 ng/mL) levels than in subjects with IFG. During the follow-up period, eight patients (8.8%) with acromegaly and 12 patients (9.8%) with IFG developed some degree of retinopathy. Three patients with acromegaly and two with IFG progressed to proliferative retinopathy. Patients with acromegaly had the same incidence of non-proliferative retinopathy (odds ratio [OR]: 0.830; 95% CI: 0.318–2.164) and a non-statistically significantly higher incidence of proliferative retinopathy (OR: 2.461; 95% CI: 0.404–14.988). The data reveals that GH and IGF-1 might play a crucial role in the development of proliferative retinopathy and influence its progression. Therefore, we suggest screening patients with acromegaly should be similar to diabetes patients.
Clinicopathological Significance of Up-Regulated Pir-1366 in NSCLC and its Effect on Tumor Migration and Metastasis
Authors: Zhou Jun , Guo Hong , Lu Wen , Shi Hongchan , Wang Chenghai 
Published: May 2021

Abstract
Background: piRNAs are a kind of noncoding RNAs that involve in tumorigenesis and development, but the role of piRNAs in NSCLC remain unclear. In this study, we explored the role of piR-1366 in NSCLC .Methods:The identification of piRNAs was performed using Human Arraystar piRNA Array in NSCLC tissues and adjacent normal tissues. up-regulated piR-1366 were validated in the NSCLC and selected for further study of migration and metastasis. In addition, the expression of piR-1366 in 87 cases of NSCLC was detected by in situ hybridization and the correlation between piR-1366 expression and pathological parameters of NSCLC patients was analyzed. meanwhile, bioinformatics, qRT-PCR and West blotting were used to examine the signal pathway of piR-1366 in migration. Results: piR-1366 was up-regulated and positive expression in NSCLC ,The positive expression of piR-1366 was closely related to lymph node metastasis of NSCLC, but not with age, gender, tumor location, tumor size, tumor grade and TNM stage. piR-1366 is transfected into lung cancer cells to promote its migration and metastasis. Mechanistically,WT1-3'UTR was complementary combination with piR-1366 by bioinformatic prediction and identified as a direct target of piR-1366 through dual-luciferase reporter assay. Over-expression and knockdown of WT1 could respectively rescue and simulate the effects induced by piR-1366. Finally, piR-1366 promoted migration and metastasis by the WT1 /CDH1 pathway in lung cancer cells. Conclusions:Thus,our article suggested that piR-1366 was a novel pro-metastasis oncogene and may represent a novel marker of diagnosis and treatment for Metastatic NSCLC.