bluuebunny/crossref_metadata_2025_split huggingface dataset:
Nuclear translocation of the tagged endogenous MAPK MPK-1 denotes a subset of activation events in C. elegans development
Authors: Rasmussen Neal R. , Reiner David J. 
Published: August 2021

ABSTRACT
The extracellular signal-regulated kinases (ERKs) are mitogen-activated protein kinases (MAPKs) that are utilized downstream of Ras to Raf to MEK signaling to control activation of a wide array of targets. Activation of ERKs is elevated in Ras-driven tumors and RASopathies, and thus is a target for pharmacological inhibition. Regulatory mechanisms of ERK activation have been studied extensively in vitro and in cultured cells, but little in living animals. In this study, we tagged the Caenorhabditis elegans ERK-encoding gene, mpk-1. MPK-1 is ubiquitously expressed with elevated expression in certain contexts. We detected cytosol-to-nuclear translocation of MPK-1 in maturing oocytes and hence validated nuclear translocation as a reporter of some activation events. During patterning of vulval precursor cells (VPCs), MPK-1 is necessary and sufficient for the central cell, P6.p, to assume the primary fate. Yet MPK-1 translocates to the nuclei of all six VPCs in a temporal and concentration gradient centered on P6.p. This observation contrasts with previous results using the ERK nuclear kinase translocation reporter of substrate activation, raising questions about mechanisms and indicators of MPK-1 activation. This system and reagent promise to provide critical insights into the regulation of MPK-1 activation within a complex intercellular signaling network.
Tissue clearing and 3D imaging – putting immune cells into context
Authors: Hofmann Julian , Keppler Selina J. 
Published: August 2021

ABSTRACT
A better understanding of cell–cell and cell–niche interactions is crucial to comprehend the complexity of inflammatory or pathophysiological scenarios such as tissue damage during viral infections, the tumour microenvironment and neuroinflammation. Optical clearing and 3D volumetric imaging of large tissue pieces or whole organs is a rapidly developing methodology that holds great promise for the in-depth study of cells in their natural surroundings. These methods have mostly been applied to image structural components such as endothelial cells and neuronal architecture. Recent work now highlights the possibility of studying immune cells in detail within their respective immune niches. This Review summarizes recent developments in tissue clearing methods and 3D imaging, with a focus on the localization and quantification of immune cells. We first provide background to the optical challenges involved and their solutions before discussing published protocols for tissue clearing, the limitations of 3D imaging of immune cells and image analysis. Furthermore, we highlight possible applications for tissue clearing and propose future developments for the analysis of immune cells within homeostatic or inflammatory immune niches.
Single motherhood, social independence and non-communicable disease (NCD) outcomes among young females (15-24 years old) in South Africa
Authors: De Wet-Billings Nicole 
Published: August 2021

Background: Non-communicable diseases (NCDs) acquired during youth follow into and affect adulthood. The association between young mother’s social independence and NCD status is of policy interest due to its effect on economic and social development. This study aimed to determine the causal relationship between social independence and NCD outcomes among young, single mothers in South Africa. Methods: Data from the South African National Income Dynamics Survey (NIDS) in 2008 and 2017 was used to determine if single mothers developed hypertension, diabetes or asthma by various indicators of social independence, including highest level of education and employment status. The sample was initially made-up of unmarried females (15-24 years old) without any children in 2008. Both fertility and social independence was followed-up to 2017. Results: In total, 66 young females developed an NCD by 2017 and 87% (n=57) of these women had a child in the interim period. Employment of young females increased from 4.78% in 2008 to 37.79% in 2017, but completion of secondary or tertiary education declined from 67.94% in 2008 to 56.01% in 2017. In addition, half (50.88%) of the young females were partially independent by 2017, with only 11.03% being fully independent at this time. Finally, logistic regression results showed that the likelihood of developing an NCD increased if young females with children were not socially independent. Conclusions: The relationship between social independence and NCDs suggest that policies and programmes in South Africa need to incorporate socioeconomic status as a determinant of disease and in particular, need to address socioeconomic indicators as additive measures and not autonomous indicators.
EFFICIENCY OF THE MODIFIED FERTILIZER AND MICROBIOLOGICAL PREPARATION ON SPRING BARLEY IN THE CONDITIONS OF THE FOREST-STEPPE OF THE VOLGA REGION
Authors: Saydyasheva Galina , Zayceva Kseniya 
Published: August 2021

Studies to determine the effect of mineral, biomineral fertilizers and biological products on the content of nutrients (nitrogen, phosphorus and potassium) during the growing season in spring barley plants were carried out in 2015-2017 on leached heavy loam chernozem of Ulyanovsk region. The soil of the experimental site contained: humus 6.43...6.62 % (according to Tyurin), total nitrogen-0.26 % (according to Kjeldahl), mobile phosphorus and potassium-214 ... 228 and 101...117 mg/kg of soil, respectively (according to Chirikov), pHKCl – 6.3...6.8 (GOST 26483-85), hydrolytic acidity – 1.20...1.29 mmol/100 g of soil (according to Kappen).
The scheme of the experiment included the following options: without fertilizers (control); Bisolbiphite (seed inoculation); N15P15K15 (regular azofoska); N15P15K15m (azofoska modified with BisolbiPhite); N7,5P7,5K7,5m (azofoska modified with BisolbiPhite in a half dose). We used a complex mineral fertilizer-azofoska in a dose of 15 kg d. v. per 1 ha of nitrogen, phosphorus and potassium and a microbiological preparation BisolbiFit, which serves as a modifier of seeds and mineral fertilizers. The drug is based on a strain of bacteria of the rhizosphere Bacillus subtilis H-13, isolated from chernozem soil. Inoculation of spring barley seeds with a biological preparation was carried out 1...2 days before sowing (400...600 g/t of seeds), mineral fertilizer was treated on the day of its application to the soil (4 kg/t of fertilizers). As the studied crop, the variety of spring barley Nutans 553, zoned in Ulyanovsk region, was sown on experimental plots. Mineral fertilizers and biological preparation had a positive effect on the nutrient content of spring barley plants. Under the influence of the studied fertilizer, the nitrogen content in the green mass of barley increased by 0.09...0.30%, phosphorus − by 0.09...0.18%, potassium-by 0.25...1.10 %, in relation to the variant where mineral fertilizer and microbiological preparation were not used. As a result of the correlation analysis, a positive relationship was established between the yield of barley and the content of nutrients in plants during the tillering, tubing and flowering phases: with nitrogen (r = 0.78; 0.83; 0.75), phosphorus (r = 0.80; 0.42; 0.71) and potassium (r = 0.66; 0.59; 0.91). As a result of this research work, it was found that the use of the studied fertilizers and the biological product BisolbiFit positively affected the content of the main nutrients (nitrogen, phosphorus and potassium) in spring barley plants in all phases of its development.
Applicability of TIVAP versus PICC in non-hematological malignancies patients: A meta-analysis and systematic review
Authors: Liu Baiying , Wu Zhiwei , Lin Changwei , Li Liang , Kuang Xuechun 
Published: August 2021

Background
Applicability of totally implantable venous access port (TIVAP) and peripherally inserted central venous catheter (PICC) in non-hematological malignancies patients remains controversial.

Methods
A systematic studies search in the public databases PubMed, EMBASE, Wan Fang, CNKI (China National Knowledge Infrastructure), the Cochrane Library and Google Scholar (updated to May 1, 2020) was performed to identify eligible researches. All statistical tests in this meta-analysis were performed using Stata 12.0 software (Stata Corp, College Station, TX). A P value less than 0.05 was considered statistically significant.

Results
Thirteen studies were included in this final meta-analysis. The pooled data showed that compared with PICC, TIVAP was associated with a higher first-puncture success rate (OR:2.028, 95%CI:1.25–3.289, P<0.05), a lower accidental removal rate (OR:0.447, 95%CI:0.225–0.889, P<0.05) and lower complication rates, including infection (OR:0.570, 95%CI: 0.383–0.850, P<0.05), occlusion (OR:0.172, 95%CI:0.092–0.324, P<0.05), malposition (OR:0.279, 95%CI:0.128–0.608, P<0.05), thrombosis (OR:0.191, 95%CI, 0.111–0.329, P<0.05), phlebitis (OR:0.102, 95%CI, 0.038–0.273, P<0.05), allergy (OR:0.155, 95%CI:0.035–0.696, P<0.05). However, no difference was found in catheter life span (P>0.05) and extravasation (P>0.05). Moreover, TIVAP is more expensive compared with PICC in six-month use (weighted mean difference:3.132, 95%CI:2.434–3.83, P<0.05), but is much similar in 12 months use (P>0.05).

Conclusion
For the patients with non-hematological malignancies, TIVAP was superior to PICC in the data related to placement and the incidence of complications. Meanwhile, TIVAP is more expensive compared with PICC in six-month use, but it is much similar in twelve-month use.
A systematic review and meta-analysis on international studies of prevalence, mortality and survival due to coal mine dust lung disease
Authors: Lu Cynthia , Dasgupta Paramita , Cameron Jessica , Fritschi Lin , Baade Peter 
Published: August 2021

Background
Coal mine dust lung disease comprises a group of occupational lung diseases including coal workers pneumoconiosis. In many countries, there is a lack of robust prevalence estimates for these diseases. Our objective was to perform a systematic review and meta-analysis of published contemporary estimates on prevalence, mortality, and survival for coal mine dust lung disease worldwide.

Methods
Systematic searches of PubMed, EMBASE and Web of Science databases for English language peer-reviewed articles published from 1/1/2000 to 30/03/2021 that presented quantitative estimates of prevalence, mortality, or survival for coal mine dust lung disease. Review was conducted per PRISMA guidelines. Articles were screened independently by two authors. Studies were critically assessed using Joanna Briggs Institute tools. Pooled prevalence estimates were obtained using random effects meta-analysis models. Heterogeneity was measured using the I2 statistics and publication bias using Egger’s tests.

Results
Overall 40 studies were included, (31 prevalence, 8 mortality, 1 survival). Of the prevalence estimates, fifteen (12 from the United States) were retained for the meta-analysis. The overall pooled prevalence estimate for coal workers pneumoconiosis among underground miners was 3.7% (95% CI 3.0–4.5%) with high heterogeneity between studies. The pooled estimate of coal workers pneumoconiosis prevalence in the United States was higher in the 2000s than in the 1990s, consistent with published reports of increasing prevalence following decades of declining trends. Sub-group analyses also indicated higher prevalence among underground miners, and in Central Appalachia. The mortality studies were suggestive of reduced pneumoconiosis mortality rates over time, relative to the general population.

Conclusion
The ongoing prevalence of occupational lung diseases among contemporary coal miners highlights the importance of respiratory surveillance and preventive efforts through effective dust control measures. Limited prevalence studies from countries other than the United States limits our understanding of the current disease burden in other coal-producing countries.
Polypharmacy prevalence in older adults seen in United States physician offices from 2009 to 2016
Authors: Young Eric H. , Pan Samantha , Yap Alex G. , Reveles Kelly R. , Bhakta Kajal 
Published: August 2021

Background/objectivesWith an aging population suffering from increased prevalence of chronic conditions in the United States (U.S.), a large portion of these patients are on multiple medications. High-risk medications can increase the risk for drug-drug interactions and medication nonadherence. This study aims to describe the prevalence of polypharmacy and high-risk medication prescribing in U.S. physician offices.MethodsThis was a cross-sectional study of the Centers for Disease Control and Prevention’s National Ambulatory Medical Care Survey from 2009 to 2016. All patients over 65 years old were included. Polypharmacy was categorized as no polypharmacy (< 2 medications), minor polypharmacy (2–3 medications), moderate polypharmacy (4–5 medications), and major polypharmacy (>5 medications). Medications were further categorized into high-risk medication categories (anticholinergics, cardiovascular agents, central nervous system (CNS) medications, pain medications, and other). Comparisons between the degrees of polypharmacy were performed utilizing chi-square or Wilcoxon rank-sum tests with JMP Pro 14®(SAS Institute, Cary, NC).ResultsOver 2 billion patient visits were included. Overall, Polypharmacy was common (65.1%): minor polypharmacy (16.2%), moderate polypharmacy (12.1%), and major polypharmacy (36.8%). Patients with major polypharmacy were older compared to those with moderate or minor polypharmacy (75 vs. 73 years, respectively) and were most frequently prescribed pain medications (477.3 per 1,000 total visits). NSAIDs were the most frequently prescribed, with 232.4 per 1,000 total visits resulting in one high-risk NSAID prescription, while 21.9 per 1,000 total visits resulted in two or more high-risk NSAIDs.ConclusionMost patients over 65 years experienced some degree of polypharmacy, with many experiencing major polypharmacy. This indicates an increased need for expanded pharmacist roles through medication therapy management and safety monitoring in this patient population.
Reduction in hospitalised COPD exacerbations during COVID-19: A systematic review and meta-analysis
Authors: Alqahtani Jaber S. , Oyelade Tope , Aldhahir Abdulelah M. , Mendes Renata Gonçalves , Alghamdi Saeed M. , Miravitlles Marc , Mandal Swapna , Hurst John R. 
Published: August 2021

Background
Reports have suggested a reduction in exacerbations of chronic obstructive pulmonary disease (COPD) during the coronavirus disease 2019 (COVID-19) pandemic, particularly hospital admissions for severe exacerbations. However, the magnitude of this reduction varies between studies.

Method
Electronic databases were searched from January 2020 to May 2021. Two independent reviewers screened titles and abstracts and, when necessary, full text to determine if studies met inclusion criteria. A modified version of the Newcastle-Ottawa Scale was used to assess study quality. A narrative summary of eligible studies was synthesised, and meta-analysis was conducted using a random effect model to pool the rate ratio and 95% confidence intervals (95% CI) for hospital admissions. Exacerbation reduction was compared against the COVID-19 Containment and Health Index.

Results
A total of 13 of 745 studies met the inclusion criteria and were included in this review, with data from nine countries. Nine studies could be included in the meta-analysis. The pooled rate ratio of hospital admissions for COPD exacerbations during the pandemic period was 0.50 (95% CI 0.44–0.57). Findings on the rate of community-treated exacerbations were inconclusive. Three studies reported a significant decrease in the incidence of respiratory viral infections compared with the pre-pandemic period. There was not a significant relationship between exacerbation reduction and the COVID-19 Containment and Health Index (rho = 0.20, p = 0.53).

Conclusion
There was a 50% reduction in admissions for COPD exacerbations during the COVID-19 pandemic period compared to pre-pandemic times, likely associated with a reduction in respiratory viral infections that trigger exacerbations. Future guidelines should consider including recommendations on respiratory virus infection control measures to reduce the burden of COPD exacerbations beyond the pandemic period.
Mortality risk prediction in high-risk patients undergoing coronary artery bypass grafting: Are traditional risk scores accurate?
Authors: Goncharov Maxim , Mejia Omar Asdrúbal Vilca , Perez de Souza Arthur Camila , Orlandi Bianca Maria Maglia , Sousa Alexandre , Praça Oliveira Marco Antônio , Atik Fernando Antibas , Coelho Segalote Rodrigo , Gradim Tiveron Marcos , Melo de Barros e Silva Pedro Gabriel , Arruda Nakazone Marcelo , Ferreira Lisboa Luiz Augusto , Oliveira Dallan Luís Alberto , Zheng Zhe , Hu Shengshou , Biscegli Jatene Fabio 
Published: August 2021

Background
The performance of traditional scores is significantly limited to predict mortality in high-risk cardiac surgery. The aim of this study was to compare the performance of STS, ESII and HiriSCORE models in predicting mortality in high-risk patients undergoing CABG.

Methods
Cross-sectional analysis in the international prospective database of high-risk patients: HiriSCORE project. We evaluated 248 patients with STS or ESII (5–10%) undergoing CABG in 8 hospitals in Brazil and China. The main outcome was mortality, defined as all deaths occurred during the hospitalization in which the operation was performed, even after 30 days. Five variables were selected as predictors of mortality in this cohort of patients. The model’s performance was evaluated through the calibration-in-the-large and the receiver operating curve (ROC) tests.

Results
The mean age was 69.90±9.45, with 52.02% being female, 25% of the patients were on New York Heart Association (NYHA) class IV and 49.6% had Canadian Cardiovascular Society (CCS) class 4 angina, and 85.5% had urgency or emergency status. The mortality observed in the sample was 13.31%. The HiriSCORE model showed better calibration (15.0%) compared to ESII (6.6%) and the STS model (2.0%). In the ROC curve, the HiriSCORE model showed better accuracy (ROC = 0.74) than the traditional models STS (ROC = 0.67) and ESII (ROC = 0.50).

Conclusion
Traditional models were inadequate to predict mortality of high-risk patients undergoing CABG. However, the HiriSCORE model was simple and accurate to predict mortality in high-risk patients.
Staging Fairyland: Folklore, Children’s Entertainment, and Nineteenth-Century Pantomime, Jennifer Schacker (2018)
Authors: Davidson Hilary 
Published: August 2021

Review of: Staging Fairyland: Folklore, Children’s Entertainment, and Nineteenth-Century Pantomime, Jennifer Schacker (2018)
Detroit: Wayne State University, 284 pp.,
ISBN 978-0-81434-590-0, p/bk, $29.99
MAPPING THE CONSUMERS COFFEE POWDER MANGSI COFFEE BASED ON SEGMENTING, TARGETING, AND POSITIONING IN DENPASAR CITY
Authors: Pratama I Putu Agi , Dewi Ratna Komala , Artini Ni Putu 
Published: August 2021

Not all customers can be served by the company. Each company needs to identify marketsegments that can be served effectively by differentiating the main market segments, aiming at one ortwo segments and developing products so that there are always new breakthroughs. A company in orderto excel in competition must be able to recognize its market segment, target and product position againstits competitors. This research aims to determine the segmenting, targeting, and positioning of MangsiCoffee powder. The sampling technique used is the nonprobability sampling method that is accidentialsampling. Segmenting and targeting is done by using crosstab analysis, while positioning uses multidimensional scaling analysis and correspondence analysis. Mangsi Coffee powder market segmentationbased on the characteristics of consumers are men who are adults, graduated from tertiary education (last education), work as employees with monthly expenditure above the Denpasar City UMK in 2019(Rp. 2,553,000.00). Mangsi Coffee consumers based on psychographic segmentation tend to choosequality products and make coffee consumption habits a trend and lifestyle while Mangsi Coffeeconsumers based on segmentation of consumer behavior tend to choose products according to theirbenefits. Positioning using multi-dimensional scaling analysis (MDS) shows that, Mangsi Coffeepowder is not in one quadrant with all three competing products. The map shows that all four productsare in different quadrants. CA analysis (correspondence analysis), shows the superiority of MangsiCoffee powder products when compared with competitors' products is an attribute in terms ofpackaging. The importance of companies to pay attention to segmenting, targeting, and positioning tobe able to focus on achieving company goals and survive in fierce market competition. The strategy toincrease sales is carried out by adjusting the results of studies in research and the conditions of theMangsi Coffee company so that it can take policies that are in accordance with company goals.
THE RELATION ANALYSIS OF MARKETING MIX AND CUSTOMER PURCHASE OF COFFEE IN MALABAR MOUNTAIN CAFÉ IN BOGOR CITY
Authors: Ateta Ernalsalia , Susrusa Ketut Budi , Arisena Gede Mekse Korri 
Published: August 2021

The aims of this research are finding out the correlation between product and consumer purchasing decisions, price and consumer purchasing decisions, promotion and consumer purchasing decisions, place and consumer purchasing decisions. This research has already done form May until July 2020. The samples were 50 respondents taken by nonprobability sampling technique. Data were collected by questionnaires. Data analysis were descriptive analysis technique and Spearman’s Correlation analysis using SPSS 23 software. The results of this research shows the product is strongly related with the correlation coeffecient value positive, which means it has a directional correlation and product has a significant correlation with the consumer purchasing decision at Malabar Mountain Cafe in Bogor. The price is strongly related with the correlation coeffecient value positive, which means it has a directional correlation and price has a significant correlation with the consumer purchasing decision at Malabar Mountain Cafe in Bogor. The promotion is strong enough related with the correlation coeffecient value positive, which means it has a directional correlation and promotion has a significant correlation with the consumer purchasing decision at Malabar Mountain Cafe in Bogor. The place is very weakly related with the correlation coeffecient value negative, which means it has undirectional correlation and the place has not a significant correlation with the consumer purchasing decision at Malabar Mountain Cafe in Bogor.
The Impact of the Lockdown on the Greek Economy and the Role of the Recovery Fund
Authors: Economides George , Philippopoulos Apostolis , Vassilatos Vanghelis 
Published: August 2021

Abstract
We develop a microfounded macroeconomic model that embeds the key features of the Greek economy. After calibrating the model to Greek data over 1995–2019, we assume that the economy is initially in the year 2019 and then quantify the adverse economic impact of the lockdown measures taken to control the spread of the pandemic, as well as the implications of the various policy measures (at national and EU level) taken to cushion the impact of the economic hit. We give quantitative answers to questions like: What will be the size and duration of the economic downturn? What are the implications of the national fiscal stimulus? What will be the role of the fiscal transfers coming from the European Recovery Fund? Our results imply that the national fiscal stimulus package adopted so far is helpful but, for the Greek economy to enter an era of sustainable growth, a mix of policies is also needed that combines: (i) a growth-enhancing fiscal mix (ii) product market deregulation (iii) a socially productive use of the resources coming from the Recovery Fund.