bluuebunny/crossref_metadata_2025_split huggingface dataset:
CD40 Ligand Blockade for Prevention of Graft-Versus-Host Disease
Authors: Khimani Farhad , Ali Haris , Kim Jongphil , Cubitt Chris , Zhang Shumin , Elmariah Hany , Perez Lia , Bejanyan Nelli , Mirza Sayeef , Perna Fabiana , Jain Michael , Lazaryan Aleksandr , Liu Hien , Alsina Melissa , Castaneda Omar , Hansen Doris , Ochoa Leonel , Betts Brian , Nishihori Taiga , Locke Frederick , Mishra Asmita , Davila Marco , Nieder Michael , Anasetti Claudio , Choe Hannah , Pidala Joseph 
Published: February 2025

PURPOSE
This phase I-Ib trial evaluated a novel CD40L blocking antibody, BMS-986004, for the prevention of graft-versus-host disease (GVHD) after unrelated donor allogeneic hematopoietic cell transplantation (HCT).

PATIENTS AND METHODS
A total of 34 patients were treated at three centers. The safety and biologic activity of single-dose BMS-986004 (675 mg [n = 6] and 1,500 mg [n = 6]; one-time dose) were evaluated. Safety of prolonged dosing was then examined in separate cohorts (drug administration every 2 weeks × 3 [n = 3], 5 [n = 3] or 7 doses [n = 16]). Included patients received 8/8 HLA-matched unrelated donor peripheral blood stem-cell HCT and sirolimus/tacrolimus GVHD prophylaxis. Comprehensive HCT outcome data were collected through 2 years, inclusive of GVHD outcomes, immune reconstitution and infections, and biologic correlative studies.

RESULTS
BMS-986004, a single, one-time dose of 1,500 mg was identified as the safe and biologically active single-dose regimen, and prolonged dosing cohorts demonstrated safety through the seven-dose duration regimen. In phase Ib (n = 16), grade II-IV acute GVHD was 25% with no grade III or IV acute GVHD. Moderate-severe chronic GVHD at 2 years was 18.4%. Considering all patients (n = 34), a total of five deep venous thrombosis (DVT) events occurred and no significant association was detected between DVT and serial D-dimer and TAT levels. There was no evidence for excess infectious complications or cytomegalovirus reactivation. Two-year estimates were the following: Non-relapse mortality 15.3% (95% CI, 5.4% to 29.8%), relapse 22% (95% CI, 9.5% to 37.8%), and overall survival 77.8% (95% CI, 58.7% to 88.8%).

CONCLUSION
This trial demonstrates that next-generation CD40L blocking antibodies can be used safely in HCT recipients and supports initial clinical efficacy in the prevention of GVHD. Further study is needed in larger populations to verify these outcomes.
Continuum electrostatics with capped Coulomb potential
Authors: Bossa Guilherme Volpe , Ehlert Braden , May Sylvio 
Published: February 2025

Abstract
For ensembles of spatially extended ions, electrostatic interaction energies can be calculated more accurately using a capped Coulomb potential, which remains constant within a fixed distance and then decays inversely with distance. When this fixed distance is small, the capped Coulomb potential transitions into a composite Coulomb-Yukawa potential, widely used in modeling dense electrolytes and ionic liquids and associated with a fourth-order derivative in Poisson's equation. To go beyond the fourth order, we develop a theoretical framework for continuum electrostatics based on the capped Coulomb potential. We derive and solve a modified Poisson equation and calculate corresponding electrostatic interaction energies. Solutions of the modified Poisson equation are shown to emerge from applying a differential operator to the potential predicted by the unmodified Poisson equation. We demonstrate that the electrostatic potential satisfying the modified Poisson equation can exhibit discontinuities at interfaces.
The spatial choreography of mRNA biosynthesis
Authors: Ventura-Gomes André , Carmo-Fonseca Maria 
Published: February 2025

ABSTRACT
Properly timed gene expression is essential for all aspects of organismal physiology. Despite significant progress, our understanding of the complex mechanisms governing the dynamics of gene regulation in response to internal and external signals remains incomplete. Over the past decade, advances in technologies like light and cryo-electron microscopy (Cryo-EM), cryo-electron tomography (Cryo-ET) and high-throughput sequencing have spurred new insights into traditional paradigms of gene expression. In this Review, we delve into recent concepts addressing ‘where’ and ‘when’ gene transcription and RNA splicing occur within cells, emphasizing the dynamic spatial and temporal organization of the cell nucleus.
Economic Evaluation of Inguinal Versus Ilio-inguinal Lymphadenectomy for Patients with Stage III Metastatic Melanoma to Groin Lymph Nodes: Evidence from the EAGLE FM Randomized Trial
Authors: Mahumud Rashidul Alam , Law Chi Kin , Ospino Daniel Ariza , de Wilt Johannes H. W. , van Leeuwen Barbara L. , Allan Chris , de Lima Vazquez Vinicius , Jones Rowan Pritchard , Howle Julie , Spillane Andrew J. , Morton Rachael Lisa 
Published: February 2025

Abstract

Purpose
We compared health outcomes and costs of inguinal lymphadenectomy (IL) versus ilio-inguinal lymphadenectomy (I-IL) for removal of metastatic melanoma to lymph nodes of the groin in adults with stage III melanoma.

Methods
A within-trial cost-utility analysis was performed alongside an international randomized trial (EAGLE-FM) with 36 months follow-up from a health system perspective. Healthcare costs were measured by using trial records, and effectiveness measured in quality-adjusted life years (QALYs). Deterministic sensitivity analyses assessed the impact of changes in costs or quality of life on overall results. Statistical bootstrapping was employed to estimate confidence intervals around the cost-utility ratio.

Results
Among 98 trial participants (IL n = 50, I-IL n = 48), with no pelvic or distant disease clinically or on PET/CT imaging, the mean life years saved for those randomized to IL showed a small but nonsignificant increase of 0.05 years compared with those in the I-IL group (2.56 vs. 2.51 years, 95% confidence interval [CI] –0.78 to 0.87). The mean difference in QALYs gained showed a small but nonsignificant increase of 0.04 QALYs (1.95 vs. 1.91, 95% CI –0.49 to 0.57). The mean hospital stay among IL patients was 6.16 days, 1.24 days shorter than I-IL patients (7.40 days) at 36 months follow-up. Mean per-patient healthcare costs of IL surgery were AU$6938 lower than for I-IL surgery ($26,555 vs. $33,493, 95% CI –$24,360 to $10,484). Inguinal lymphadenectomy was slightly more effective and slightly less expensive) over I-IL; a finding supported by 81% of bootstrapped estimates and upheld across sensitivity analyses.

Conclusions
Our study indicates that less extensive IL surgery might be the preferred surgical strategy for metastatic melanoma to the groin when PET/CT imaging shows no pelvic disease. This surgery is likely to improve quality-adjusted survival and may reduce healthcare costs; however, the differences noted in EAGLE-FM were limited by a small sample size and were not statistically significant.

Trial Registration
Clinicaltrials.gov NCT02166788; anzctr.org.au ACTRN12614000721606.
Defining and Institutionalizing Proactive Advising: Insights from a National Survey
Authors: Rust Matthew M. , Willey Ann Elizabeth 
Published: February 2025

Abstract
Calls to adopt proactive advising as a student success strategy are common, but evidence on what this entails is scarce. We present results from a national survey on the conceptualization and institutionalization of proactive advising at four-year U.S. colleges and universities. Examining differing views among advisors and administrators, we identify advising practices, implementation strategies, and technologies deemed absent but necessary to institutionalize proactive advising. Through multilevel modeling, we estimate that absent-but-needed implementation strategies have the greatest effect on respondent impression of institutionalization, and that the effects of absent-but-needed technologies depend on respondent role. Summarizing findings from regression and content analyses, we propose a comprehensive definition of proactive advising and recommend successful institutionalization through coordinated rather than decentralized efforts.
Antigen-specific T cell responses following single and co-administration of tick-borne encephalitis, Japanese encephalitis, and yellow fever virus vaccines: Results from an open-label, non-randomized clinical trial-cohort
Authors: Wullimann David , Sandberg John Tyler , Akber Mira , Löfling Marie , Gredmark-Russ Sara , Michaëlsson Jakob , Buggert Marcus , Blom Kim , Ljunggren Hans-Gustaf 
Published: February 2025

Background
Flavivirus infections pose a significant global health burden, highlighting the need for safe and effective vaccination strategies. Co-administration of different vaccines, including licensed flavivirus vaccines, is commonly practiced providing protection against multiple pathogens while also saving time and reducing visits to healthcare units. However, how co-administration of different flavivirus vaccines de facto affects immunogenicity, particularly with respect to T cell responses, is only partially understood.

Methods and findings
Antigen-specific T cell responses were assessed in study participants enrolled in a previously conducted open-label, non-randomized clinical trial. In the trial, vaccines against tick-borne encephalitis virus (TBEV), Japanese encephalitis virus (JEV), or yellow fever virus (YFV) were administered either individually or concomitantly in different combinations in healthy study participants. Peripheral blood samples were collected before vaccination and at multiple time points afterward. To analyze antigen-specific CD4+ and CD8+ T cell responses, PBMCs were stimulated with overlapping peptide pools from TBEV, JEV, YFV, and Zika virus (ZIKV) envelope (E), capsid (C), and non-structural protein 5 (NS5) viral antigens. A flow cytometry-based activation-induced marker (AIM) assay was used to quantify antigen-specific T cell responses. The results revealed remarkably similar frequencies of CD4+ and CD8+ T cell responses, regardless of whether vaccines were administered individually or concomitantly. In addition, administering the vaccines in the same or different upper arms did not markedly affect T cell responses. Finally, limited cross-reactivity was observed between the TBEV, JEV, and YFV vaccines, and related ZIKV-specific antigens.

Conclusions
TBEV or JEV vaccines can be co-administered with the live attenuated YFV vaccine without any markedly altered antigen-specific CD4+ and CD8+ T cell responses to the respective flaviviruses. Additionally, the vaccines can be delivered in the same or different upper arms without any significant altered influence on the T cell response. From a broader perspective, these results provide valuable insights into the outcome of immune responses following simultaneous administration of different vaccines for different but related pathogens.
Barriers to and facilitators of rehabilitation according to socio-economic status, after acute respiratory distress syndrome due to COVID-19: A qualitative study in the RECOVIDS cohort
Authors: Kléber Mathilde , Meunier-Beillard Nicolas , Fournel Isabelle , Ksiazek Eléa , Jacquier Marine , Ecarnot Fiona , Rigaud Jean-Philippe , Declerq Pierre-Louis , Quenot Jean-Pierre , Labruyère Marie 
Published: February 2025

Background
The COVID-19 pandemic may have compounded social disparities in access to healthcare, with possible deleterious consequences on the functional prognosis of patients after a stay in the intensive care unit (ICU). In the previous RECOVIDS study, we reported that despite comparable pulmonary sequelae and similar access to rehabilitation, socio-economically “vulnerable” patients had lower quality of life at 6 months after an ICU stay. We aimed to describe the barriers to, and facilitators of participation in rehabilitation, among patients from the RECOVIDS study, regardless of their socio-economic situation.

Methods
Qualitative study using semi-structured interviews with adult patients admitted to ICU for PCR-proven SARS-CoV-2 infection, and who had acute respiratory distress syndrome (ARDS) or had received high flow nasal oxygen. In addition, patients had to have been living at home for the month prior to the interview and had to be proficient in French. Eligible patients were randomly selected, aiming to select the same number of socially deprived and non-socially-deprived patients. Interviews were transcribed for thematic analysis.

Results
In total, 31 interviews were performed from 10/2021 to 01/2022; 16 with socially deprived, and 15 with non-deprived participants. Average age was 65.2 (±11.6) years. Four themes emerged from the analysis of the interviews, namely: (1) the impact of the patient’s professional and socio-economic situation; (2) the feeling that age and socio-economic situation influence access to rehabilitation; (3) a perception that the healthcare system was saturated, and that inequalities exist in access to rehabilitation resources; (4) perception of previous own health and expectations of post-resuscitation health status.

Conclusion
A precarious socio-economic situation has a substantial impact on access to rehabilitation after ICU admission for ARDS caused by COVID-19. It represents a barrier to rehabilitation through the combined action of various social determinants that deserve to be detected early, in order to take appropriate action to ensure that the most socially vulnerable individuals can benefit from access to rehabilitation.
Contribution of health system governance in delivering primary health care services for universal health coverage: A scoping review
Authors: Khatri Resham B , Endalamaw Aklilu , Erku Daniel , Wolka Eskinder , Nigatu Frehiwot , Zewdie Anteneh , Assefa Yibeltal 
Published: February 2025

Background
The implementation of the primary health care (PHC) approach requires essential health system inputs, including structures, policies, programs, organization, and governance. Effective health system governance (HSG) is crucial in PHC systems and services, as it can significantly influence health service delivery. Therefore, understanding HSG in the context of PHC is vital for designing and implementing health programs that contribute to universal health coverage (UHC). This scoping review explores how health system governance contributes to delivering PHC services aimed at achieving UHC.

Methods
We conducted a scoping review of published evidence on HSG in the delivery of PHC services toward UHC. Our search strategy focused on three key concepts: health system governance, PHC, and UHC. We followed Arksey and O’Malley’s scoping review framework and adhered to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses extension for Scoping Reviews (PRISMA-ScR) checklist to guide our methodology. We used the World Health Organization’s framework on HSG to organize the data and present the findings.

Results
Seventy-four studies were included in the final review. Various functions of HSG influenced PHC systems and services, including:1) formulating health policies and strategic plans (e.g., addressing epidemiological and demographic shifts and strategic financial planning), 2) implementing policy levers and tools (such as decentralization, regulation, workforce capacity, and supply chain management), 3) generating intelligence and evidence (including priority setting, monitoring, benchmarking, and evidence-informed decision-making), 4) ensuring accountability (through commitments to transparency), and 5) fostering coordination and collaboration (via subnational coordination, civil society engagement, and multisectoral partnerships). The complex interplay of these HSG interventions operates through intricate mechanisms, and has synergistic effects on PHC service delivery.

Conclusion
PHC service delivery is closely linked to HSG functions, which include formulating strategic policies and plans responsive to evolving epidemiological and demographic needs, utilizing digital tools, decentralizing resources, and fostering multisectoral actions. Effective policy implementation requires robust regulation, evidence-based decision-making, and continuous monitoring. Accountability within health systems, alongside community engagement and civil society collaboration, is vital for realizing PHC principles. Local health institutions should collaborate with communities—end users of these systems—to implement formal rules and ensure PHC service delivery progresses toward UHC. Sociocultural contexts and community values should inform decision-making aligning health needs and services to achieve universal access to PHC services.
Acceptability of long-acting antiretroviral therapy among people living with HIV who use drugs in Vancouver, Canada: A qualitative study
Authors: Chayama Koharu Loulou , Ng Cara , Brohman Isabella , Mansoor Manal , Small Will , Philbin Morgan , Collins Alexandra B. , McNeil Ryan 
Published: February 2025

Background
People living with HIV (PLHIV) who use drugs face overlapping social-structural inequities that contribute to suboptimal adherence to antiretroviral therapy (ART). Recent research suggests that long-acting antiretroviral therapy (LA-ART) can offer an important alternative to daily oral ART by mitigating adherence barriers, but this work has largely excluded PLHIV who use drugs. We, therefore, explored the acceptability of injectable and transdermal LA-ART among PLHIV who use drugs in Canada, which has no-cost universal access to oral ART. Greater understanding of PLHIV who use drugs’ perspectives on LA-ART will be essential to fully leverage treatment advances and maximize its individual- and population-level benefits.

Methods
From February 2019 to February 2020, we conducted in-depth interviews with 33 PLHIV who use drugs in Vancouver, Canada with suboptimal ART adherence (i.e., ≦  50%). Participants were recruited for interviews through a prospective cohort study. Interviews were audio-recorded, transcribed, and analyzed using inductive and deductive approaches.

Results
Our analysis identified key factors shaping LA-ART acceptability. First, LA-ART enthusiasm was highest among participants who were less satisfied with oral ART regimens due to pill burden or adverse side effects for oral ART and participants who struggled with daily adherence due substance use and social-structural factors (e.g., homelessness, incarceration). Moreover, participants who had physicians they trusted identified their physicians as credible sources of information on LA-ART, and indicated a desire for informed and shared decision-making regarding treatment changes. Finally, participants emphasized that there is no one-size-fits-all treatment approach for PLHIV who use drugs and highlighted the importance of access to a full range of available treatment options, including LA-ART.

Conclusions
There is potential for high LA-ART uptake among PLHIV who use drugs, particularly those who experience adherence barriers due to their substance use and structural vulnerability. While our findings are limited by the specific population and setting, they nevertheless underscore the need for efforts to ensure universal and equitable access to LA-ART.
Endoscopic submucosal excavation for gastric muscularis propria tumours less than 10 mm in diameter: What are the risk factors responsible for perforation?
Authors: Liu Zhaohui , Li Hualin , Deng Jiwen , Wu Ruinuan 
Published: February 2025

Objective
To explore the risk factors for perforation during endoscopic submucosal excavation (ESE) for gastric muscularis propria tumours less than 10 mm in diameter. This study provides clinical guidance for preventing the occurrence of intraoperative adverse events.

Methods
Samples of gastric muscularis propria tumours less than 10 mm in diameter were removed via ESE at Shenzhen Second People’s Hospital and were collected from June 2023 to August 2024. The general clinical characteristics of the patients, location, size, growth pattern, and pathology of the tumours, operation time, resection time, perforation incidence and bleeding incidence were analysed, and logistic regression was used to calculate the risk factors for perforation and bleeding.

Results
A total of 102 patients were included in this study. The tumours were successfully removed from all patients. The mean age was 52.28 ± 11.84 years. There were 34 (33.33%) males. The mean size was 6.96 ± 1.82 mm. 89 (87.25%) tumours exhibited an intraluminal growth pattern. In total, 79 (77.45%) tumours were in the gastric body, and 23 (22.55%) tumours were in the gastric fundus. The mean operation time was 35.26 ± 23.15 min, and the mean resection time was 27.88 ± 21.77 min. A total of 55 (53.92%) tumours were leiomyoma, and 43 (42.16%) tumours were GIST. There were 4 (3.92%) tumours classified as other lesions. 41 (40.20%) patients experienced intraoperative bleeding, all of which had minor bleeding and successful haemostasis under endoscopy.There were 27 (26.47%) concurrent perforation, of which,24(88.89%) were diagnosed as GIST, and 3(11.11%) were diagnosed as leiomyoma. All perforations were successfully managed with an endoscopic suture. According to the multivariate regression analysis, a pathologic diagnosis was a risk factor for perforation. When the pathological diagnosis is GIST, the risk of perforation increases (PE = 18.632, 95% CI 4.571 ~ 75.941; p < 0.001). Gender,age,tumor size, growth pattern, location, and resection time were not found to be risk factors for perforation.all of observed factors were not the risk factors for bleeding.

Conclusion
ESE is an effective removal method for gastric muscularis propria tumours less than 10 mm in diameter.Intraoperative bleeding and perforation are common.However, these complications are controllable.GIST is an independent risk factor for the perforation.When the tumour is diagnosed as a GIST, the incidence of perforation is significantly increased.
The use of tranexamic acid for post-tonsillectomy hemorrhage: A scoping review protocol
Authors: Tesema Naomi , Hasnie Sukaina , Earley Marisa , April Max 
Published: February 2025

Background
Post-tonsillectomy hemorrhage (PTH) is a highly studied outcome of tonsillectomy with serious consequences. Various treatments and interventions have been utilized to decrease post-tonsillectomy hemorrhage. The off-label use of tranexamic acid (TXA) is of growing interest to control PTH but has not been incorporated in management guidelines. This scoping review plans to summarize existing studies from the scientific literature on the use of tranexamic acid for post-tonsillectomy hemorrhage.

Methods

We used the Preferred Reporting Items for Systematic Reviews or Meta-Analysis Extension for Scoping Reviews (PRISMA-ScR). The review will cover studies including patients undergoing tonsillectomy who were treated with TXA in the peri—operative or post-operative period. We include randomized controlled trials, retrospective, prospective, and case series. A database-specific search strategy will be used to search records across. Two reviewers will independently screen and extract data. Tables and visual representations will be utilized to present the extracted data.

Registration details

The protocol will be registered in Open Science Framework and published in PLOS One.