bluuebunny/crossref_metadata_2025_split huggingface dataset:
Spatially regulated protease activity in lymph nodes renders B cell follicles a sanctuary for retention of intact antigens
Authors: Aung Aereas , Cui Ang , Soleimany Ava P. , Bukenya Maurice , Lee Heya , Cottrell Christopher A. , Silva Murillo , Kirkpatrick Jesse D. , Gregory Justin R. , Amlashi Parastoo , Remba Tanaka , Froehle Leah M. , Xiao Shuhao , Abraham Wuhbet , Adams Josetta , Suh Heikyung , Huyett Phillip , Kwon Douglas S. , Hacohen Nir , Schief William R. , Bhatia Sangeeta N. , Irvine Darrell J. 
Published: November 2021

SUMMARYThe structural integrity of vaccine antigens is critical, as antigen degradation in vivo could eliminate neutralizing epitopes and create competing B cell responses against irrelevant breakdown products. Using FRET imaging and imaging zymography, we found that protease activity and antigen breakdown are spatially heterogeneous in lymph nodes. Following protein immunization, antigens are rapidly degraded in the subcapsular sinus, paracortex, and interfollicular regions of the tissue. By contrast, the follicles and follicular dendritic cell (FDC) networks exhibit low protease activity and antigen degradation rates. Immunization regimens targeting antigen rapidly to FDCs led to germinal centers (GCs) where responses to intact antigen were highly dominant, while traditional bolus immunizations led to weaker GC responses where more GC B cells bound to breakdown products than intact antigen. Thus, spatially-compartmentalized antigen proteolysis impacts humoral immunity and can be exploited to enhance vaccine-induced production of antibody responses against key pathogen structural epitopes.
Built on sand: the shaky foundations of simulating single-cell RNA sequencing data
Authors: Crowell Helena L. , Morillo Leonardo Sarah X. , Soneson Charlotte , Robinson Mark D. 
Published: November 2021

AbstractWith the emergence of hundreds of single-cell RNA-sequencing (scRNA-seq) datasets, the number of computational tools to analyse aspects of the generated data has grown rapidly. As a result, there is a recurring need to demonstrate whether newly developed methods are truly performant – on their own as well as in comparison to existing tools. Benchmark studies aim to consolidate the space of available methods for a given task, and often use simulated data that provide a ground truth for evaluations. Thus, demanding a high quality standard for synthetically generated data is critical to make simulation study results credible and transferable to real data.Here, we evaluated methods for synthetic scRNA-seq data generation in their ability to mimic experimental data. Besides comparing gene- and cell-level quality control summaries in both one- and two-dimensional settings, we further quantified these at the batch- and cluster-level. Secondly, we investigate the effect of simulators on clustering and batch correction method comparisons, and, thirdly, which and to what extent quality control summaries can capture reference-simulation similarity.Our results suggest that most simulators are unable to accommodate complex designs without introducing artificial effects; they yield over-optimistic performance of integration, and potentially unreliable ranking of clustering methods; and, it is generally unknown which summaries are important to ensure effective simulation-based method comparisons.
Cryo-EM of the yeast VOcomplex reveals distinct binding sites for macrolide V-ATPase inhibitors
Authors: Keon Kristine A. , Benlekbir Samir , Kirsch Susanne H. , Müller Rolf , Rubinstein John L. 
Published: November 2021

AbstractVacuolar-type adenosine triphosphatases (V-ATPases) are proton pumps found in almost all eukaryotic cells. These enzymes consist of a soluble catalytic V1region that hydrolyzes ATP and a membrane-embedded VOregion responsible for proton translocation. V-ATPase activity leads to acidification of endosomes, phagosomes, lysosomes, secretory vesicles, and the trans-Golgi network, with extracellular acidification occurring in some specialized cells. Small molecule inhibitors of V-ATPase have played a crucial role in elucidating numerous aspects of cell biology by blocking acidification of intracellular compartments, while therapeutic use of V-ATPase inhibitors has been proposed for treatment of cancer, osteoporosis, and some infections. Here, we determine structures of the isolated VOcomplex fromSaccharomyces cerevisiaebound to two well-known macrolide inhibitors: bafilomycin A1 and archazolid A. The structures reveal different binding sites for the inhibitors on the surface of the proton-carrying c ring, with only a small amount of overlap between the two sites. Binding of both inhibitors is mediated primarily through van der Waals interactions in shallow pockets and suggests that the inhibitors block rotation of the ring. Together, these structures indicate the existence of a large chemical space available for V-ATPase inhibitors that block acidification by binding the c ring.
Osmotic dehydration as a pre-treatment for jackfruit berries (Artocarpus heterophyllus): effect on physico-chemical properties, shelf life and sensory acceptability
Authors: Alves Evaristo Raquel , Costa Gilmar Freire da , Grisi Cristiani Viegas Brandão , de Sousa Solange 
Published: November 2021

PurposeThe aim of the study was to evaluate the effect of osmo-convective dehydration using two drying temperatures (50 and 60°C) on the physico-chemical properties, shelf life and sensory acceptability of jackfruit berries.Design/methodology/approachFour treatments were used as follows: T1 – Jackfruit berries dried in an oven at 50°C; T2 – Jackfruit berries dried in an oven at 60°C; T3 – Jackfruit berries pre-treated with an osmotic-sucrose solution (60 Brix concentration) and oven-dried at 50°C and T4 – Jackfruit berries pretreated with osmotic solution (60 Brix concentration) and oven-dried at 60°C. Berries' shelf life was monitored by moisture analysis, water activity (Wa) and microbiological analysis. The influence of treatments on physico-chemical properties and sensory quality was also investigated.FindingsOsmo-convective dehydration at 50 and 60 C significantly (p = 0.001) reduced moisture content and Wa. All treatments increased the shelf life of jackfruit berries within 30 days of storage. Regarding sensory analysis, the T3 treatment was significantly (p = 0.001) the most preferred by the tasters.Originality/valueIn general, the results indicated that osmo-convective dehydration at 50 C has great potential to be a maid in the processing of minimally processed jackfruit berries, as, in addition to providing the product with high stability, it was the one with the greatest acceptability by the tasters.
The influence of risks on the outturn cost of ICT infrastructure network projects
Authors: Almarri Khalid , Boussabaine Halim , Al Nauimi Hamad 
Published: November 2021

Purpose
The internet of things (IoT) is becoming an increasingly inescapable part of society. IoT paradigm cannot function without the networking infrastructure. High-speed data networks are essential to enable the IoT future. Thus, the purpose of this study is on the identification of risks that influence the development, installation and operation of information and communication technology (ICT) infrastructure network project cost outcomes. So far, there has been little attention has been paid to risks problems in these types of IoT enabling projects.

Design/methodology/approach
This research follows a quantitative analysis approach. Data for this study were collected by a survey from 209 professionals. Multiple regression analysis was used to model the relationship between risks and outturn cost of infrastructure needed to enable the operation of IoT technologies.

Findings
The main risk factors that were identified were planning and development, people and management, operations, technology and hardware.

Research limitations/implications
This research has expanded the existing literature by documenting and clustering ICT infrastructure network project risks into themes, and has developed a scale (risk statements) for measuring such risks. Further, the research has advanced the understanding by identifying the most likely risks that will contribute to the overrun of these projects.

Originality/value
This research establishes a reliable regression method for the assessment of the risks that influence the development, installation and operation of ICT infrastructure network projects outturn cost. No other research has measured or studied the risks in this type of project.
The architecture of the phygital customer journey: a dynamic interplay between systems of insights and systems of engagement
Authors: Mele Cristina , Russo-Spena Tiziana 
Published: November 2021

Purpose
This paper aims to focus on how companies shape the architecture of a phygital customer journey through the exploitation of smart technologies.

Design/methodology/approach
The research adopts a qualitative method using a grounded theory approach involving leading players in digital customer solutions and service providers from different industries.

Findings
The shaping of the architecture of the phygital customer journey comes from the interplay between systems of insights and systems of engagement activated by multiple customer-provider interactions in an entanglement of physical and digital contexts.

Practical implications
Companies need to design a blended approach to bridge disconnected contexts, capture new opportunities and provide customer engagement along the entire journey.

Originality/value
This study depicts the “phygital customer journey” under systems of insights and systems of engagement: These systems operate as dynamic architectures to capture insights and engage customers.
An adaptive wavelet collocation method for the optimal heat source problem
Authors: Khaksar-e Oshagh Mahmood , Abbaszadeh Mostafa , Babolian Esmail , Pourbashash Hossein 
Published: November 2021

Purpose
This paper aims to propose a new adaptive numerical method to find more accurate numerical solution for the heat source optimal control problem (OCP).

Design/methodology/approach
The main aim of this paper is to present an adaptive collocation approach based on the interpolating wavelets to solve an OCP for finding optimal heat source, in a two-dimensional domain. This problem arises when the domain is heated by microwaves or by electromagnetic induction.

Findings
This paper shows that combination of interpolating wavelet basis and finite difference method makes an accurate structure to design adaptive algorithm for such problems which usually have non-smooth solution.

Originality/value
The proposed numerical technique is flexible for different OCP governed by a partial differential equation with box constraint over the control or the state function.
Gathering pre-purchase information for a cruise vacation with virtual reality: the effects of media technology and gender
Authors: Martínez-Molés Víctor , Jung Timothy Hyungsoo , Pérez-Cabañero Carmen , Cervera-Taulet Amparo 
Published: November 2021

Purpose
This study aims to apply theory on consumer learning in virtual experiences to compare how media technologies (i.e. virtual reality [VR] and standard websites) and users’ gender influence the ways in which tourists gather pre-purchase information.

Design/methodology/approach
A laboratory experiment with fully immersive VR was conducted to examine consumers’ behavior in gathering pre-purchase information. The sample comprised 128 consumers who had taken a cruise vacation or who were considering purchasing a cruise package in the near future.

Findings
The results generally reveal the central role of the feeling of presence, which, in turn, positively impacts users’ enjoyment and aspects of consumer learning (i.e. brand attitude, product knowledge and purchase intent). In particular, the results suggest that compared with standard websites, VR facilitated the tourists’ learning as consumers, especially among women, who tend to dominate the information-gathering stage of planning family vacations.

Practical implications
The results imply that travel agencies and tourism centers working with cruise vacation companies should incorporate VR to make their offers more attractive, especially to women.

Originality/value
The study was the first to apply theory on consumer learning in the cruise tourism industry, specifically to compare fully immersive VR devices versus standard websites and gauge the effect of gender.
Artificial intelligence and creative activities inside organizational behavior
Authors: Paesano Andrea 
Published: November 2021

Purpose
This study aims to investigate about the use of artificial intelligence (AI) (man machine relationship) regarding organizational behavior. The aim of this research paper is to analyze whether the current AI is used also to replace man in “creative” activities.

Design/methodology/approach
This study is based on a qualitative and explorative approach. It is made a review of the literature with “Scopus” and “Web of Science” databases. The research fields are AI, organizational behavior, man-machine relationship and creativity.

Findings
Analyzing whether the intensive use of AI in organizational behavior can replace human work in creative activities.

Research limitations/implications
The connection of AI with creative activities within the organization is only just beginning. For this reason, other sources, like Harvard Business Review, public reports and professional papers found on the internet have been considered. The most important limitation of this paper is that all the results presented here do not concern a single case study.

Practical implications
In this paper, there are some examples that can show the use of AI in creative activities; however, this does not complete the situation facing companies in any sector because the AI technologies used within enterprises are constantly evolving. It is possible to continue to do research in this field.

Originality/value
The paper is meaningful because highlights the development of AI toward creative activities typically of human resources. It is also interesting because it analyzes the exploratory use of AI in increasingly human work, generating positive and negative externalities.
Global knowledge centrality: co-inventor collaboration with China
Authors: Salmon Jessica , Zaman Salma , Satoglu Emine Beyza , Sanchez-Henriquez Fernando , Velez-Calle Andres 
Published: November 2021

PurposeThis paper examines the role of co-inventor collaboration with China and/or the USA on a country's increase in centrality in global knowledge networks. It also explores the role of specific institutional factors – corruption and intellectual property rights (IPR) protection – on this relationship.Design/methodology/approachIn the study, co-inventor data from the United States Patent and Trademark Office (USPTO) applications have been used to construct networks of technological knowledge collaboration at the country level over the years 2002–2015. Using eigenvector centrality as the dependent variable, the study uses fixed effect regression analyses on a panel of 171 countries, contributing to recent debates on knowledge networks and international cooperation.FindingsBuilding on research in economic development, innovation and social network theory, this research finds that co-patenting with Chinese inventors is positively related to a country's centrality in global knowledge networks and that this relationship is negatively moderated by collaboration with the current most central knowledge network – namely that of the USA – suggesting a substitution effect. It also finds a partial substitution between institutional factors, IPRs protection and transparency, and collaboration with China on a country's knowledge centrality.Practical implicationsRegarding policymakers, the findings can be used to encourage international collaboration for increased access to new sources of knowledge that fosters innovation while keeping a close eye on local institutions, especially emerging economies that want to increase their international knowledge network centrality.Originality/valueThis study creates a unique panel data set and extends the social networks approach in international business literature, focusing on institutional characteristics related to participation in knowledge networks.
Performance improvements in logistics outsourcing relationships: the role of LSP and customer organizational culture archetypes
Authors: Nevries Fabian , Wallenburg Carl Marcus 
Published: November 2021

PurposeThe study aims to develop an organizational culture typology and explore how different logistics service provider (LSP) and customer archetypes interact to generate performance improvements in logistics outsourcing relationships.Design/methodology/approachA multiple case study approach with 12 dyads was employed. Interviews as well as public and internal data from LSPs and customers were analyzed.FindingsThe results reveal four archetypes each for LSPs and customers, characterized by two dimensions: “activeness” and “openness”. Furthermore, analyzing the interaction among the archetypes, three relationship patterns are identified (“static”, “restrained”, and “progressive”) that differ in the exploratory and exploitative improvement outcomes.Research limitations/implicationsThe study contributes to theory development at the intersection of organizational culture and logistics outsourcing.Originality/valueThe study provides a typology of organizational culture in logistics outsourcing and how different archetypes interact to generate improvements.
Heritage potential's mobilisation: challenges and development prospects the case of Guelma in Algeria
Authors: Zerti Mouna , Benrachi Bouba Lezzar 
Published: November 2021

PurposeThe region of Guelma, located in the northeast of Algeria, is characterised by its important natural and cultural heritage. This heritage is ignored and abandoned, which leads to its degradation and even its disappearance. Thus, it is imperative to seek to know and understand the reasons for this situation. This study aims to reveal the constraints of heritage promotion and its staging through its insertion in the development process.Design/methodology/approachA hypothetical–deductive approach based on a qualitative method using surveys was carried out on the knowledge of the heritage of Guelma by the actors concerned. The laws and classification procedures were interpreted to identify the heritage's stakes to ensure sustainable local development through tourism.FindingsThe results show the lack of recognition of the natural and cultural heritage by the region's population, the cumbersome, long and complex classification procedure. The weakness of the offer in terms of tourist infrastructures constitutes a handicap to the valorisation of the heritage and prevents the enhancement and development of tourism. Systematically, heritage does not contribute to the local and territorial development of the Guelma region.Originality/valueThe article underlines the importance of raising awareness among the various heritage stakeholders and actors, and first, the population, of the value of their natural and cultural heritage to make it profitable and a driving force for local and territorial development.
Does holding offshore jurisdictions to higher AML standards really assist in preventing money laundering?
Authors: Perkins Andrew James 
Published: November 2021

Purpose
This paper aims to contend that when tackling financial crimes such as money laundering and terrorist financing, international regulators are seeking to hold offshore jurisdictions such as the Cayman Islands to higher standards and that this detracts from the pursuit of detecting and prosecuting money launders.

Design/methodology/approach
This paper will deal with the following perceived issues: firstly, to offshore jurisdictions as a concept; secondly, to outline the efforts made by the Cayman Islands to combat money laundering and to rate these changes against Financial Action Task Forces’ (FATAF’s) technical criteria; thirdly, to demonstrate that the Cayman Islands is among some of the world’s top jurisdictions for compliance with FATAF’s standards; and finally, to examine whether greylisting was necessary and to comment upon whether efforts by international regulators to hold offshore jurisdictions to higher standards detracts from the actual prosecution of money laundering within the jurisdiction.

Findings
Greylisting the Cayman Islands in these authors’ view was something that should have never happened; the Cayman Islands is being held to standards far beyond what is expected in an onshore jurisdiction. There is a need for harmonisation in respect of international anti money laundering rules and regulations to shift the tone to prosecution and investigation of offences rather than on rating jurisdictions technical compliance with procedural rules where states have a workable anti-money laundering (AML) regime.

Research limitations/implications
The implications of this research are to show that offshore jurisdictions are being held by FATAF and other international regulators to higher AML standards than their onshore counterparties.

Practical implications
The author hopes that this paper will begin the debate as to whether FATAF needs to give reasons as to why offshore jurisdictions are held to higher standards and whether it needs to begin to contemplate higher onshore standards.

Originality/value
This is an original piece of research evaluating the effect of FATAF's reporting on offshore jurisdictions with a case study involving primary and secondary data in relation to the Cayman Islands.
Employee ownership – pros and cons – a review
Authors: Mygind Niels , Poulsen Thomas 
Published: November 2021

PurposeThe purpose of this paper is to give an updated overview of the research on employee ownership. What does the scientific literature reveal about advantages and disadvantages? What can be learned from different models used in Italy, France, Mondragon (Spain), UK and US with many employee-owned firms in contrast to Denmark.Design/methodology/approachA structured review of the literature on employee. The paper identifies different mechanisms leading to effects on productivity, job stability, distribution, investment etc., and reviews the empirical evidence. The main barriers and drivers are identified and different models for employee ownership in Italy, France, Mondragon (Spain), UK and US are reviewed to identify potential models for a country like Denmark with few employee-owned firms.FindingsThe article gives an overview over the theoretical predictions and the main empirical evidence of the effects of employee ownership. The pros are greater employee identification with the firm and increased productivity reinforced by increased participation. Employee-owned firms have more equal distribution of wages and more stable employment, and they have greater mutual control between employees and fewer middle managers. The motivation effects may be smaller for large firms and lack of capital may lead to lower levels of investments and capital per employee.Originality/valueComprehensive and updated literature review on the effects and successful formats of employee ownership to identify models for implementation in countries with few employee-owned firms.