bluuebunny/crossref_metadata_2025_split huggingface dataset:
Phenotypic intrafamilial variability including H syndrome and Rosai Dorfman Disease associated with the same c.1088G&gt;A mutation in the SLC29A3 gene.
Authors: CHOUK Hamza , REJEB Mohamed BEN , BOUSSOFARA Lobna , MABROUK Haïfa EL , GHARIANI Najet , SR Badreddine , SAAD Ali , H'MIDA Dorra , DENGUEZLI Mohamed 
Published: July 2021

Abstract
Background: Mutations in the SLC29A3 gene, which encodes the nucleoside transporter hENT3, has been implicated in syndromic forms of Histiocytosis including H syndrome, pigmented hypertrichosis with insulin-dependent diabetes, Faisalabad histiocytosis and Familial Rosai Dorfman disease (RDD). Herein, we report five new patients from a single family who present with phenotypes that associate features of H syndrome and Familial Rosai Dorfman Disease.Methods: We investigated the clinical, biochemical, histopathological and molecular findings in five Tunisian family Members' diagnosed as Familial RDD and/or H syndrome. The solute carrier family 29 (nucleoside transporters), member 3 ( SLC29A3 ) gene was screened for molecular diagnosis using direct Sanger sequencing.Results: Genetic analysis of all affected individuals revealed a previously reported missense mutation c.1088 G>A [p.Arg363Gln] in exon 6 of the SLC29A3 gene. Four affected members presented with clinical features consistent with classical H syndrome phenotype. While their cousin’s features were in keeping with the diagnosis of Familial Rosai Dorfman disease with a previously undescribed cutaneous RDD presenting as erythematous nodular plaques on the face. This report underlines the clinical variability of SLC29A3 disorders even with an identical mutation in the same family.Conclusion: We report a rare event of 5 Tunisian family Members' found to be homozygous for SLC29A3 gene mutations but showing a different phenotype severity. Our study reveals that despite a single mutation, the clinical expression of the SLC29A3 disorders may be significantly heterogeneous suggesting a poor genotype-phenotype correlation for the disease.
Source Tracking of Carbapenem-Resistant Enterobacteriaceae Outbreaks in Neonatal Patients Revealed Rivers Contaminated with NDM Genes
Authors: Wang Jianshjeng , Yin Changfu , Lv Yuanpeng , Yang Weiwei , Zhao Peng 
Published: July 2021

Abstract
Background: Carbapenem-resistant Enterobacteriaceae (CRE) prevalence in neonatal patients continues to rise in china. However, very few epidemiological data are available to uncover the underlying mechanisms for the increase. This study aimed to elucidate their transmission modes and explore potential sources. Methods: The genotypic features of CRE isolates were analyzed by PCR and sequencing. The elucidation of transmission and sources involved in electronic medical record review, environmental cultures, screening cultures, and prospective case-control analysis. The transferability of carbapenem-resistant genes was performed by Conjugation experiments.Results: A total of 450 CRE isolates recovered from 369 infant patients were genotypically characterised. Carbapenemase genes NDM-1, KPC-2 and IMP-4 accounted for 86.22% (n = 388), 8.89% (n = 40) and 4.44% (n = 20), respectively. After implementation of intensified control measures, CRE rectal prevalence rates in both the 0-3 days and the 4-28 days age group were decreased from 15.53% (100/644) and 27.37% (52/190) to 11.78% (51/433) and 19.59% (19/97) (P = 0.082 and P = 0.148), respectively. Multivariate analysis showed that gastric lavage (odds ratio [OR]) 3.09, 95% confidence interval [CI] 1.49–6.39) and enema (OR 2.84, 95% CI 1.65–4.91) were independent risk factors for rectal colonisation in the 0-3 days age group, whereas Cephalosporins (OR 2.12, 95% CI 1.14–3.94), gastric lavage (OR 3.44, 95% CI 1.44–8.22) and residence in rural area (OR 2.22, 95% CI 1.19–4.13) were independently associated with rectal colonization in the 4-28 days age group. The transmission tracking of neonatal NDM-1 strains outbreaks revealed rivers contaminated with NDM genes. Most isolates with NDM genes could be successfully transferred.Conclusion: Continued vigilance for importations should be maintained to reduce CRE threat.
An Integrated Routing Mechanism on Homogenous Wireless Sensor Networks using Genetic Algorithm
Authors: Danehchin Ramin 
Published: July 2021

Abstract
Data collection on Wireless Sensor Networks (WSNs) is a significant challenge to satisfy the requirements of various applications. Providing an energy-efficient routing technique is the primary step in data collection over WSNs. The existing data collection techniques in the WSNs field struggle with the imbalance load distribution and the short lifetime of the network. This paper proposes a novel mechanism to select cluster-heads, cluster the wireless sensor nodes, and determine the optimal route from source nodes to the sink. We employ the genetic algorithm to solve the routing problem considering the hop-count of the cluster-heads to the sink, the number of each cluster member, residual energy of cluster-heads, and the number of cluster-heads connected to the sink as the fitness criteria. Our proposed mechanism uses a greedy approach to calculate the hop-count of each cluster-head to the sink for integrating the clustering and routing process on WSNs. The simulation results demonstrate that our proposed mechanism improves the energy consumption, the number of live nodes, and the lifetime of the network compared to other data collection approaches on WSNs.
Physiological And Proteomic Analyses Reveals That Brassinosteroids Application Improves The Chilling Stress Tolerance of Pepper Seedlings
Authors: Li Jie , Sohail Hamza , Nawaz Muhammad Azher , Liu Chaowei , Yang Ping 
Published: July 2021

Abstract
Brassinosteroids (BRs) are important in plant resistance to chilling stress. However, limited information is available regarding the specific mechanisms involved at proteomic level. We utilized iTRAQ proteomic approach, physiological assays and information obtained from cellular ultrastructure to clarify the underlying molecular mechanism of BRs to alleviate chilling stress in pepper (Capsicum annuum L.). Foliar application of 24-epibrassinolide (EBR) improved photosynthesis and improved cell structure by presenting a distinct mesophyll cell and chloroplast with well-developed thylakoid membranes in the leaves of pepper seedlings. We identified 346 differentially expressed proteins (DEPs), including 217 up-regulated proteins and 129 down-regulated proteins in plants under chilling (Chill) and Chill + EBR treated plants. Most of the DEPs were related to multiple pathways, including photosynthesis, carbohydrates metabolism, energy metabolism, protein biosynthesis, amino acids synthesis, redox and stress defence (ascorbate peroxidase, glutathione peroxidase, and superoxide dismutase). Up-regulated DEPs were associated with photosynthetic electron transfer chain, oxidative phosphorylation, GSH metabolism pathway, Calvin cycle and signaling pathway. The physiochemical analysis showed that EBR treatment improved the tolerance of pepper seedlings to chilling stress.
Presurgical fMRI Affecting the Extent of Resection and Outcome of the Supratentorial Gliomas; a Single-center Experience From a Developing Country
Authors: Mortazavi Abolghasem , Azad Mohammad Ali Suraki , Amirjamshidi Abbas , Shirani Mohammad , Ketabchi Seyed Ebrahim , Yarandi Kourosh Karimi , Pour-Rashidi Ahmad 
Published: July 2021

Abstract
BackgroundFunctional magnetic resonance imaging (fMRI) is one of the available non-invasive imaging to map the different brain areas, which has been used during the current years. In this study, we aimed to evaluate the effect of fMRI on decision-making, the extent of resection, and the outcome of the patients with supratentorial gliomas.MethodsThis prospective study conducted from 2014 through 2017 to investigate how presurgical fMRI can help the neurosurgeon during glioma surgery. Baseline demographic and clinical data were collected, and standard fMRI protocol was used for each patient.Results Forty-one patients with supratentorial gliomas met inclusion criteria, including 29 males and 12 females ranging in age from 21 to 72. Our results showed a significant association between eloquent areas tumor and the EOR. Eight out of 41 (19.5%) experienced higher EOR than what the neurosurgeon expected after adjusting the fMRI findings with the intraoperative situation. Among these patients, postoperative KPS and motor indices reflected dropped levels accompanied by an instant language index level in the short-term period, whereas all indices improved after long-term follow-up. There was a significant association between increasing EOR and all indices in the 1-year follow-up.ConclusionsIn conclusion, we postulate that preoperative fMRI should be considered preoperatively, especially in the eloquent areas gliomas disregarding tumor grade. Moreover, it can lead to a positive outcome in supratentorial gliomas via giving useful data about the relation of the tumor and vital centers of the brain, although it may result in an unfavorable short-term outcome.
CircTP63 Promotes Cell Proliferation and Invasion by Regulating EZH2 via Sponging miR-217 in Gallbladder Cancer
Authors: Wang Shouhua , Tong Huanjun , Su Tingting , Zhou Di , Shi Weibin , Tang Zhaohui , Quan Zhiwei 
Published: July 2021

Abstract
Background: Gallbladder cancer (GBC) is the most common biliary tract malignancy and has a poor prognosis in patients with GBC. CircRNA TP63 (circTP63) has been implicated in some tumor proliferation and invasion in some tumors. The study aims to investigate the clinical significance and functional role of circTP63 in GBC.Methods: The expression of circTP63 in GBC was detected by qRT-PCR and the association between circTP63 expression and prognosis of GBC patients was analyzed. CCK8 assay, flow cytometry analysis, transwell assay and in vivo studies were used to evaluated the cell proliferation and invasion after circTP63 knockdown in GBC cells. Luciferase reporter assays and RNA pull-down assay were used to determine the correlation between circTP63 and miR-217. Besides, western blot analysis was also performed.Results: In the present study, we showed that circTP63 expression was upregulated in GBC tissues and cells. Higher circTP63 expression was associated with lymph node metastasis and short overall survival (OS) in patients with GBC. In vitro, knockdown of circTP63 inhibited cell proliferation, cell cycle progression, migration and invasion in GBC. Besides, we demonstrated that knockdown of circTP63 inhibited GBC cell EMT process. In vivo, knockdown of circTP63 inhibited tumor growth in GBC. Mechanistically, we demonstrated that circTP63 competitively bind to miR-217 and promoted EZH2 expression and finally facilitated tumor progression.Conclusions: Our findings demonstrated that circTP63 sponge miR-217 and regulated EZH2 expression and finally facilitates tumor progression. Thus, targeting circTP63 may be a therapeutic strategy for the treatment of GBC.
Decreased Cases of Pediatric Intussusception During COVID-19
Authors: Park Jun Sung , Byun Young-Hoon , Choi Seung Jun , Lee Jong Seung , Ryu Jeong-Min , Lee Jeong-Yong 
Published: July 2021

Abstract
Objectives: Coronavirus disease 2019 (COVID-19) changed the epidemiology of various diseases. The present study retrospectively investigated the epidemiologic and clinical changes of intussusception from before to after the COVID-19 outbreak, when communicable diseases other than COVID-19 have dramatically decreased due to social lockdown.Methods: The incidence of intussusception and the total monthly visits, including communicable and non-communicable diseases, aged ≤7 years, were investigated from February 2019 to January 2021 in a single pediatric emergency department of a university-affiliated tertiary hospital. The relationship between the incidence of communicable disease and intussusception was analyzed. The clinical characteristics of patients with intussusception were compared for the one-year periods before (February 2019–January 2020) and after (February 2020–January 2021) social lockdown due to COVID-19.Results: Incidence of communicable disease, non-communicable disease, and intussusception is decreased after COVID-19 outbreak (15,932 to 3,880 [24.4%], 12,994 to 8,050 [62.0%], and 87 to 27 [31.0%], respectively). The incidence of intussusception correlated significantly with the change in incidence of communicable diseases (Poisson log-linear regression, Odds ratio=2.15, 95% CI=1.08–4.26, P=0.029). Compared with non-pandemic period, patients of pandemic period showed higher proportions of pathologic leading point and hospitalization (14.8% vs. 2.3%, 18.5% vs. 4.6%, respectively), lower base excess (-4.8 mmol/L vs. -3.6 mmol/L), and higher lactate concentration (1.7 mmol/L vs. 1.5 mmol/L).Conclusions: The incidence of pediatric intussusception decreased after the COVID-19 pandemic. This reduced incidence may be related to the reduced incidence of communicable diseases. However, the proportions of more severe disease and PLPs were higher after the COVID-19 pandemic.
Multifocal Electroretinogram Changes after Vitrectomy in Patients with Diabetic Macular Edema
Authors: Elsalhy Fathy , Khalil Hisham Fawzy , Ibrahim Mohamed Mohamed-Aly 
Published: July 2021

Abstract
Purpose: was to assess the results of vitrectomy with and without internal limiting membrane (ILM) peeling on retinal function and anatomy in patients with diabetic macular edema (DME). Methods: Pars plana vitrectomy (PPV) was done in 40 eyes of 40 patients with DME (15 male, 25 female). Patients were enrolled randomly into two different groups. Group A- comprised (20) eyes who underwent vitrectomy without ILM peeling. Group B- comprised (20) eyes who underwent vitrectomy with ILM peeling. For each patient, visual acuity (VA) examination (in decimal charts), assessment of central macular thickness (CMT) with optical coherence tomography (OCT) and multifocal eletroretinogram (MF-ERG) were done before and 3 months post vitrectomy. Results: Postoperative mean VA increased significantly (from 0.17 to 0.27 in group A and from 0.21 to 0.38 in group B). Mean CMT decreased significantly after surgery (from 493 μm to 315 μm in group A and from 502 μm to 299 μm in group B). Mean P1 wave amplitude (nV/ deg2) in the macular area increased after surgery (from 32.15 to 41.10 in group A and from 35.5 to 40.8 in group B). Mean P1 wave latency (millisecond) in the macular area decreased after surgery (from 50.30 to 36.40 in group A and from 49.5 to 39.2 in group B).Conclusion: Vitrectomy with and without ILM peeling improve VA and macular edema in diabetic patients moreover an increase of amplitude and reduction of the latency of the macular mf-ERGs indicated an improvement of the macular visual function.
Assessment of Neonicotinoid Insecticide Imidacloprid LC50 And Their Toxicity Parameters Against Earthworm (Eisenia Fetida)
Authors: Gill Parveen , Rao Sudhakara , Gupta Rajender , Singh Dharambir , Dahiya Tejpal , Lather Deepika , Kumar Naresh 
Published: July 2021

Abstract
Because of their high biomass in the soil, earthworms are used as bio-indicator species for assessing soil toxicity against pesticides. The regular observed sensitivity to relatively low pesticide concentrations exits in soils is a significant ecological observation. Insecticide residues harm the flora of beneficial invertebrates and harm the physiological functions of earthworms, resulting in death. They affect morphological parameters as well as internal organs, and eight different imidacloprid concentrations (0.050 µl/cm2, 0.100 µl/cm2, 0.150 µl/cm2, 0.200 µl/cm2, 0.250 µl/cm2, 0.300 µl/cm2, 0.350 µl/cm2, 0.400 µl/cm2) were prepared with water during the procedure. To establish the LC50 value, earthworms of Eisenia fetida were exposed to various concentrations of imidacloprid using the usual paper contact toxicity method, and their toxicity levels are established. The mortality percentage was estimated after 24 hours of imidacloprid exposure, and a dosage of 0.195 µl/cm2 resulted in 50% mortality of earthworms. When higher concentrations of imidacloprid were used, negative effects were observed. For ecotoxicological evaluations, the following morphological and behavioural changes were observed during the experiment: Preclittelar bulging, body constriction, blackening of the body, segment swelling, oozing of coelomic fluid, body constriction, cuticle rupture, and oozing of fluid from the body are all common side effects.
Awareness on Risk Factor for Obstetric Fistula and Associated Factors Among Reproductive Age Women in South Eastern Zone of Tigray,Ethiopia,2020.Cross Sectional Study
Authors: Teklay Berhane Asfaha , Gebru Haftu Berhe , Belay Desta Siyoum , Gebremeskel Teferi Gebru 
Published: July 2021

Abstract
Background: Worldwide, around one million girls and women are currently living with fistula. Less than 20,000 women with obstetric fistula are treated each year. Lack of awareness is a frequently mentioned barrier to seeking fistula treatment; many women suffering from obstetric fistula do not know what fistula is, as it is treatable, or where to get treatment. Even though obstetric fistula has likely weighed down women since the beginning of time, few researches proportionally exists.Methods: A community based quantitative cross-sectional survey was undertaken in south eastern zone of Tigray. A multistage random sampling technique was implemented to select total participants of 605 reproductive age women. Two districts were randomly selected and from those districts, 12 kebels were selected randomly and the calculated sample size (605) was proportionally allocated to each selected kebeles. The data were collected by using face to face/interview with structured questionnaire from February 26-Murch 24/2020 after ensuring that all requirements of ethical considerations were fulfilled. The collected data were entered in to Epidata version 4.2 then exported to SPSS version20 for analysis. Descriptive statistics with frequency, percentage, table and graph and cross tabulation were used for presentation of result. Bivariable and multivariable analysis were used to examine the association. Odds ratios with 95% confidence interval and P-value <0.05 were used to determine the statistical association.Results: Overall, about 42.15%, of respondents had good awareness on risk factors of obstetric fistula. The major determinant factors identified to awareness on risk factors of obstetric fistula were, level of education(above secondary) [AOR (95%CI=2.7(1.189-6.24)], having history of modern family planning use [AOR (95%CI=2.22(1.14-4.34)], institutional delivery (for the index child)[AOR (95%CI=2.3(1.1-4.99)] and prior information about obstetric fistula[AOR (95%CI=6.1(2.65-14.1)].Conclusion: In this study majority of reproductive age women in the study area had poor awareness regarding risk factors of obstetric fistula. Several interventions like health education and information should be implemented to enhance the awareness of the community towards risk factors for obstetric fistula.
Cut-Off Low Patterns and Its Simultaneous Precipitation Over Iran
Authors: Azizi Ghasem , Rezaei Hossein 
Published: July 2021

Abstract
Iran located in arid and semiarid area of the earth with low precipitation. Iran's rainfall is mainly received in the cold period of the year. Although the total annual precipitation is low but sometime, the country receives heavy rainfall. One of the conditions that lead to heavy rainfall is cut-off low patterns. Cut-off low-pressure systems are defined as closed lows in the upper troposphere that have become completely detached from the main westerly current. The characteristics of Cut-off Lows in Iran are studied for the period of 1976–2015. To identify these systems, the Raul Nieto algorithm was used with automatic detection capabilities. The systematic identification of Cut-off Lows is realized by applying an original automated scheme using mean daily geo-potential height, wind and air temperature at 500 hPa NCEP. Cut-off Lows are more frequent during spring in the Iran, with a maximum of frequency in March. Over the period of 1976–2015, the annual Cut-off Lows frequency exhibits a weak positive trend. In this study, the most cut-off lows lasted only 2 days. Simultaneous precipitation with cut-off low pressures was compared with annual precipitation of stations. Northwest of Iran has a larger share of Cut-off low precipitation. Different Cut-off Low patterns were identified under 6 separate patterns. The highest frequency of cut-off lows are the eastern arm of omega pattern.
Effect of Land Cover Change and Elevation on Decadal Trend of Land Surface Temperature: A Linear Model with Sum Contrast Analysis
Authors: Abdulmana Sahidan , Lim Apiradee , Wongsai Sangdao , Wongsai Noppachai 
Published: July 2021

Abstract
Land surface temperature (LST) is a significant factor in surface energy balance and global climatology studies. Land cover (LC) and elevation are two factors that affect the change of LST, and their effects depend on different geography. This study aims to demonstrate an alternative approach to examine the change of LST during 20 years (2001 to 2020) on Taiwan Island and to investigate the effect of LC change and elevation on a decadal trend of LST using a linear model that adjusting for each determinate factor. MODIS LST and LC data, as well as GMTED2010 elevation product, were downloaded available website. The natural cubic spline function was used to model annual seasonal patterns in LST. Linear regression model was used to estimate decadal change of long-term LST time series. Weighted sum contrasts linear regression was used to assess the effect of LC transformation and elevation on the decadal LST change by comparing adjusting mean of all factors. The adopted analysis method was an appropriate approach to assess categorical factors than those based on treatment contrasts, requiring specifying a control group to compare means and confidence intervals. Results showed that there was an increase in LST for most of the island. The average daytime and nighttime LST trends were 0.12 and 0.31°C/decade, respectively. However, areas in the southern part of the north-south direction mountain range show a statistically significant increase in LST in both daytime and nighttime. The major landslides caused this noticeable change of surface temperature due to the catastrophic damage of typhoon Morakot in 2009. The results also revealed that the different pattern of LC change has a significant effect on daytime LST, but not on nighttime LST trends. The elevation above 600 m had affected both daytime and nighttime LSTs.