bluuebunny/crossref_metadata_2025_split huggingface dataset:
Chronic gastroduodenal pathology in schoolchildren: the clinical picture and features of the course
Authors: Turdieva Sh. T. , Ganieva D. K. , Abdurashidova Kh. B. 
Published: March 2021

The aim of the study was to study the course and clinical manifestations of chronic gastroduodenal pathology (CGDP) in schoolchildren.Materials and methods. Clinically examined 286 children and adolescents from 6 to 15 years old with СGDP. The research methods included collection of anamnestic data, instrumental and functional (esophagogastroduodenoscopy, pH-metry), clinical and laboratory (general blood analysis, coprology), and Helicobacter pylori infection was tested by respiratory test and fecal immunochromatographic test.Result. Studies have shown that the main clinical manifestations of СGDP in children were dyspeptic syndrome, vegetovascular dysfunction, and pain syndrome, against the background of objectively visible signs of anemia. Symptoms were more characteristic in patients with СGDP from vegetovascular dysfunction: loss of appetite (83.6%), fatigue (83.2%), recurrent headaches (62.2%), and sleep disturbances (59.1%). Among dyspeptic disorders, unstable stools (85%), periodic nausea (57.7%), belching (56.6%), and a feeling of heaviness in the epigastric region after eating (56.3%) prevailed. Clinical symptoms more pronounced in children with chronic gastritis.Conclusion. For schoolchildren with CGDP, vegetovascular dysfunction (98.9%) with severe dyspeptic disorders (100%) and pain (79%), against the background of objective signs of anemia (53%), is more characteristic. In adolescents is more often a less symptomatic.
Ehlers-Danlos syndrome with damage to the digestive tract, heart, kidneys and other organs
Authors: Borzakova S. N. , Kharitonova L. A. , Osmanov I. M. , Maikova I. D. 
Published: March 2021

Relevance: Hereditary connective tissue disorders (HCTDs) are a heterogeneous group of diseases caused by mutations in the genes of extracellular matrix proteins or proteins involved in connective tissue morphogenesis. Mutations of these genes lead to the development of many HCTDs. The best known monogenic variants of HCTDs are Marfan syndrome, Ehlers-Danlos syndrome, and osteogenesis imperfecta. Inheritance is mainly autosomal, dominant or recessive. Although the first signs of HCTDs develop as early as the first year of life, it takes several years for pediatricians and specialty physicians to make the diagnosis of connective tissue dysplasia because of a lack of clear methodological approach. The disease is multi-morbid and may manifest under gastroenterological, cardiological, nephrological, or respiratory masks.Aim: to present a clinical case of Ehlers-Danlos syndrome with multiorgan digestive, heart, kidneys, and other lesions.Material and methods: the case history is presented of a 15‑year-old boy with Ehlers-Danlos syndrome, classic type.Discussion: defective collagen increased the connective tissue extensibility affecting function of many body organs and systems, including gastrointestinal, biliary, and urinary tracts, musculoskeletal and cardiovascular systems. Small developmental anomalies led to functional (motor) disorders, which contributed to the chronic organic pathology (erosive reflux esophagitis, gastroduodenitis, cholelithiasis, proctosigmoiditis, chronic pyelonephritis, or chronic sinusitis). Given the multi-organ character of the lesions, the progredient course of bone and joint changes, and early development of disability, the prognosis for the health of this child is serious. A multidisciplinary approach is important to plan the follow-up (with orthopedist, gastroenterologist, cardiologist, ophthalmologist, and nephrologist). Timely rehabilitation, therapeutic physical training courses, massage, metabolic, and anti-relapse treatment are necessary to slow down the progredient course of the hereditary connective tissue disease.
Differential diagnosis of gallbladder opisthorchiasis and cholelithiasis in a teenager
Authors: Kucherya T. V. , Kharitonova L. A. , Ashmanov K. Yu. , Mishkin A. A. 
Published: March 2021

Relevance. Parasitic invasions of the biliary tract, such as opisthorchiasis, are one of the significant causes of the formation of pigment calculi in the gallbladder, which is known in territories endemic for these diseases. However, visualization of the dynamics of the development of the pathological process using ultrasonography does not allow differentiating gall stones with a high degree of accuracy from the stages of development of the feline fluke. In the absence of an epidemiological history, this invasion can proceed under the guise of gallstone disease, which leads to an unjustified surgical intervention with the removal of a practically healthy gallbladder from a child. Before surgery, differentiation of calculi in the gallbladder from the stage of opisthorchiasis will significantly improve the quality of life of the child.The aim of the study was to demonstrate a clinical case of opisthorchiasis invasion in a child in a non-endemic territory, proceeding under the guise of cholelithiasis.Material and methods: the case history of a 17‑year-old girl with opistochiasis invasion is presented.Discussion. Parasitic invasion (opisthorchiasis) caused the formation of «calculi» of the gallbladder in the child. Not every hyperechoic formation of the gallbladder with the presence of a sound track is a «calculus». Timely diagnosis of the cause of cholelithiasis is important, as well as timely treatment of parasitic invasion to avoid relapse of the disease and errors in treatment, including surgical treatment.
TINJAUAN EKONOMI ISLAM TERHADAP PERILAKU KONSUMEN DALAM JUAL BELI EMAS
Authors: Rahmawati Rahmawati 
Published: March 2021

Tujuan penelitian yaitu untuk mengetahui Perilaku Konsumen dalam Jual beli emas di Toko Emas Pasar Los Kota Lhokseumawe dan mengetahui tinjauan ekonomi Islam terhadap perilaku konsumen. Penelitian ini adalah penelitian penelitian kualitatif deskriptif. Hasil penelitiannya bahwa perilaku konsumen itu dilakukan karena ingin berinvestasi, memenuhi kebutuhan dan juga ingin digunakan disaat acara penting seperti lebaran atau pernikahan yang berniat untuk memperlihatkan kemampuannya akan kemakmuran yang dimilikinya. Sehingga prilaku konsumen yang terjadi yaitu sering gonta ganti perhiasan tanpa mengingat akan harga emas ataupun kerugian dan keuntungan sehingga pembelian dilakukan secara berlebihan. Tinjauan ekonomi Islam terhadap perilaku konsumen dalam jual beli emas tidak sesuai dengan ekonomi Islam karena sifat berlebihan dan memamerkan sangat dilarang dalam Islam karena tidak dapat memberikan maslahah kepada setiap orang namun memberikan aspek kecemburuan sosial, namun jika perilaku untuk berinvestasi dan memenuhi kebutuhan maka didalam ekonomi Islam itu dibolehkan.
Estimasi Cadangan Karbon Pada Tumbuhan Bawah dan Serasah Di Hutan Pendidikan dan Penelitian Fakultas Kehutanan Universitas Mulawarman Samarinda
Authors: Hartati Wahjuni , Suhardiman Ali , Sudarmadji Triyono , Syahrinudin Syahrinudin , Sulistiyo Eko Adi 
Published: March 2021

Three slopes of 100 m long are defined in young secondary forest with gentle and slightly steep slopes. In each part of the slope, we built 1 (one) PUP of 20m x 20m which is consisted of 3 (three) Sub PUPs of 10m x 10m to represent the density of sparse, medium and dense understorey and 1m x 1m of sub-PUPs for litter harvesting. A total of 18 (eighteen) PUPs, each 54 (fifty four) Sub PUPs and Sub sub PUPs were made in this study. The HPFU area is dominated by young secondary forest coverage with gentle and slightly steep slopes. The C reserves of herbaceous understorey is greater than that of woody plants. C reserves of all components studied in the gently sloping area are greater than the steep ones. The largest C reserves is found in leaf litter for all slope positions except for a slightly steep lower slope, the largest C reserves is found in finer litter. The total C reserves of understorey and young secondary forest organic litter in HPFU 933.4 tonnes and 95.9% are stored in litter.
Acknowledgement to Reviewers of Maritime Technology and Research in 2020
Authors: Office MTR Editorial 
Published: March 2021

The editorial team greatly appreciates the reviewers who have dedicated their considerable time and expertise to the journal’s rigorous editorial process in 2020, regardless of whether the submissions were finally published or not. In 2020, a total of 45 articles were submitted to the journal, with a median time to first decision of 50 days, and 71 days from submission to publication. The editorial team would like to express their sincere gratitude to the following reviewers for their generous contribution in 2020:
Agnieszka Lazarowska, Poland
Akihiko Matsuda, Japan
Aldo Chircop, Canada
Anand Kumar, Malaysia
Anastasia Christodoulou, Sweden
Anthony Paul Sison Guerrero, United States
Anthony Yaw Karikari, Ghana
Arunachalam Ponshanmugakumar, India
Asen Asenov, Bulgaria
Birgit Pauksztat, Sweden
Boris Svilicic, Croatia
Carlos Efrén Mora Luis, Spain
Chalermpong Senarak, Thailand
Chandrashekher Umanath Rivonker, India
Che Abd Rahim Mohamed, Malaysia
Christiaan Adika Adenya, Kenya
Christopher Nolan, United States
Dimitrios Dalaklis, Sweden
Dong-Taur Su, Taiwan
Ergun Demirel, Turkey
Fatima Zohra Bouthir, Morocco
Florin Rusca, Romania
Floris Goerlandt, Canada
Fu Ming Tzu, Taiwan
Geng-Ruei Chang, Taiwan
George H. Kaplan, United States
Giulio Dubbioso, Italy
Hao Long, China
Hong Oanh Owen Nguyen, Australia
Jacopo Aguzzi, Spain
Jagan Jeevan, Malaysia
Jerónimo Esteve-Perez, Spain
Jiangang Jin, China
Jianjun Wu, China
Jianmin Li, China
Jiao Jialong, China
Juan Carlos Astudillo, Hong Kong
Jun Ando, Japan
Kantapon Tanakitkorn, Thailand
Kwan Ouyang Taiwan
Laura Piñeiro, Spain
Li Ye, China
Lidong Fan, Australia
M. P. R Prasad, India
Maciej Reichel, Poland
Mahinda Bandara, Sri Lanka
María-Araceli Losey-Leon, Spain
Marta Mańkowska, Poland
Maruj Limpawattana, Thailand
Masayoshi Doi, Japan
Mate J. Csorb, Norway
Ming-Cheng Tsou, Taiwan
Mohammed Russtam Suhrab Ismail, Malaysia
Mohd Hazmi Bin Mohd Rusli, Malaysia
Moses Kopong Tokan, Indonesia
Mumini Dzoga, Kenya
Neil J. Douglas, New Zealand
Nucharee Nuchkoom Smith, Thailand
Oghenetejiri Digun-Aweto, South Africa
Olabisi Michael Olapoju, Nigeria
Olaf Chresten Jensen, Denmark
Om Prakash Sha, India
Paul Tae-Woo Lee, China
Pengfei Zhang, United Kindom
Peter RANERI, Sweden
Peter Ralph Galicia, Philippines
Phansak Iamraksa, Thailand
Phatchara Sriphrabu, Thailand
Proshanto Mukherjee, China
Saikat Banerjee, India
Sarinya Sanitwong Na Ayutthaya, Thailand
Seonho Cho, Korea
Sheree-Ann Adams, Grenada
Supawat Chaikasem, Thailand
Surasak Phoemsapthawee, Thailand
Suresh Bhardwaj, India
Thee Chowwanonthapunya, Thailand
Vasilios D. Tsoukalas, Greece
Wirachaya Chanpuypetch, Thailand
Yodchai Tiaple, Thailand
Yottana Khunatorn, Thailand
Condições climáticas na incidência de cercosporiose (Cercospora coffeicola) e bicho-mineiro (Leucoptera coffeella) em cultivares de cafeeiros em Monte Carmelo, Minas Gerais, Brasil
Authors: Nascentes Taynara Faria , Nascimento Letícia Gonçalves do , Fernandes Marco Iony dos Santos , Dutra Winícius Baquião , Carvalho Fábio Janoni , Andaló Vanessa , Gallet Deyvid da Silva , Dias Werik Pereira , Assis Gleice Aparecida de 
Published: March 2021

O material genético adaptado às condições do Cerrado Mineiro e o monitoramento pode ser um fator determinante para o manejo eficiente de pragas e doenças do cafeeiro. Neste contexto, objetivou-se avaliar as condições climáticas na incidência de cercosporiose e bicho-mineiro em cultivares de cafeeiros na região de Monte Carmelo, Minas Gerais. O experimento foi conduzido na Universidade Federal de Uberlândia, Campus Monte Carmelo. O delineamento experimental utilizado foi em blocos casualizados, com quatro blocos, e os tratamentos foram constituídos por oito cultivares de Coffea arabica L.: Acaiá Cerrado MG-1474; Mundo Novo IAC 379-19; Bourbon Amarelo IAC J10; Catuaí Vermelho IAC 99; Paraíso MG H 419-1; Topázio MG-1190; Acauã Novo e IAC 125 RN. Cada parcela experimental foi constituída por 10 plantas, sendo avaliadas as cinco plantas centrais. Foi avaliada mensalmente a presença de lesões de cercosporiose no ramo localizado no terço superior pelo método não destrutivo. Foram contabilizadas quinzenalmente o número de folhas com minas intactas de bicho-mineiro no ramo plagiotrópico selecionado no terço médio. A incidência de cercosporiose e bicho-mineiro foi avaliada no período entre dezembro de 2017 a agosto de 2018. As cultivares que apresentaram menor incidência de bicho-mineiro foram Mundo Novo IAC 379-19, Acaiá Cerrado MG-1474, Bourbon Amarelo IAC J10, Paraíso MG H 419-1 e Topázio MG-1190, respectivamente. A maior incidência de bicho-mineiro ocorreu no período de abril até agosto. Os meses que apresentaram maior incidência de cercosporiose foram de dezembro a maio, havendo variabilidade da ocorrência da doença entre as cultivares testadas.
An Isothermal Molecular Point of Care Testing for African Swine Fever Virus Using Recombinase-Aided Amplification and Lateral Flow Assay Without the Need to Extract Nucleic Acids in Blood
Authors: Zhang Yuhang , Li Qingmei , Guo Junqing , Li Dongliang , Wang Li , Wang Xun , Xing Guangxu , Deng Ruiguang , Zhang Gaiping 
Published: March 2021

African swine fever (ASF) is a highly contagious and usually deadly porcine infectious disease listed as a notifiable disease by the World Organization for Animal Health (OIE). It has brought huge economic losses worldwide, especially since 2018, the first outbreak in China. As there are still no effective vaccines available to date, diagnosis of ASF is essential for its surveillance and control, especially in areas far from city with limited resources and poor settings. In this study, a sensitive, specific, rapid, and simple molecular point of care testing for African swine fever virus (ASFV) B646L gene in blood samples was established, including treatment of blood samples with simple dilution and boiling for 5 min, isothermal amplification with recombinase-aided amplification (RAA) at 37°C in a water bath for 10 min, and visual readout with lateral flow assay (LFA) at room temperature for 10–15 min. Without the need to extract viral DNA in blood samples, the intact workflow from sampling to final diagnostic decision can be completed with minimal equipment requirement in 30 min. The detection limit of RAA-LFA for synthesized B646L gene-containing plasmid was 10 copies/μl, which was 10-fold more sensitive than OIE-recommended PCR and quantitative PCR. In addition, no positive readout of RAA-LFA was observed in testing classical swine fever virus, porcine reproductive and respiratory syndrome virus, porcine epidemic diarrhea virus, pseudorabies virus and porcine circovirus 2, exhibiting good specificity. Evaluation of clinical blood samples of RAA-LFA showed 100% coincident rate with OIE-recommended PCR, in testing both extracted DNAs and treated bloods. We also found that some components in blood samples greatly inhibited PCR performance, but had little effect on RAA. Inhibitory effect can be eliminated when blood was diluted at least 32–64-fold for direct PCR, while only a 2–4 fold dilution of blood was suitable for direct RAA, indicating RAA is a better choice than PCR when blood is used as detecting sample. Taken together, we established an sensitive, specific, rapid, and simple RAA-LFA for ASFV molecular detection without the need to extract viral DNA, providing a good choice for point of care testing of ASF diagnosis in the future.
The PREdictor of MAlnutrition in Systemic Sclerosis (PREMASS) Score: A Combined Index to Predict 12 Months Onset of Malnutrition in Systemic Sclerosis
Authors: Bagnato Gianluca , Pigatto Erika , Bitto Alessandra , Pizzino Gabriele , Irrera Natasha , Abignano Giuseppina , Ferrera Antonino , Sciortino Davide , Wilson Michelle , Squadrito Francesco , Buch Maya H. , Emery Paul , Zanatta Elisabetta , Gangemi Sebastiano , Saitta Antonino , Cozzi Franco , Roberts William Neal , Del Galdo Francesco 
Published: March 2021

Objective: Malnutrition is a severe complication in Systemic Sclerosis (SSc) and it is associated with significant mortality. Notwithstanding, there is no defined screening or clinical pathway for patients, which is hampering effective management and limiting the opportunity for early intervention. Here we aim to identify a combined index predictive of malnutrition at 12 months using clinical data and specific serum adipokines.Methods: This was an international, multicentre observational study involving 159 SSc patients in two independent discovery (n = 98) and validation (n = 61) cohorts. Besides routine clinical and serum data at baseline and 12 months, Malnutrition Universal Screening Tool (MUST) score and serum concentration of leptin and adiponectin were measured for each participant at baseline. The endpoint of malnutrition was defined according to European Society of Clinical Nutrition and Metabolism (ESPEN) recommendation. Significant parameters from univariate analysis were tested in logistic regression analysis to identify the predictive index of malnutrition in the derivation cohort.Results: The onset of malnutrition at 12 months correlated with adiponectin, leptin and their ratio (A/L), MUST, clinical subset, disease duration, Scl70 and Forced Vital Capaciy (FVC). Logistic regression analysis defined the formula: −2.13 + (A/L*0.45) + (Scl70*0.28) as the best PREdictor of MAlnutrition in SSc (PREMASS) (AUC = 0.96; 95% CI 0.93, 0.99). PREMASS &lt; −1.46 had a positive predictive value (PPV) &gt; 62% and negative predictive value (NPV) &gt; 97% for malnutrition at 12 months.Conclusion: PREMASS is a feasible index which has shown very good performance in two independent cohorts for predicting malnutrition at 12 months in SSc. The implementation of PREMASS could aid both in clinical management and clinical trial stratification/enrichment to target malnutrition in SSc.
Network Analysis of the CSF Proteome Characterizes Convergent Pathways of Cellular Dysfunction in ALS
Authors: Thompson Alexander G. , Gray Elizabeth , Charles Philip D. , Hu Michele T. M. , Talbot Kevin , Fischer Roman , Kessler Benedikt M. , Turner Martin R. 
Published: March 2021

BackgroundAmyotrophic lateral sclerosis is a clinical syndrome with complex biological determinants, but which in most cases is characterized by TDP-43 pathology. The identification in CSF of a protein signature of TDP-43 network dysfunction would have the potential to inform the identification of new biomarkers and therapeutic targets.MethodsWe compared CSF proteomic data from patients with ALS (n = 41), Parkinson’s disease (n = 19) and healthy control participants (n = 20). Weighted correlation network analysis was used to identify modules within the CSF protein network and combined with gene ontology enrichment analysis to functionally annotate module proteins. Analysis of module eigenproteins and differential correlation analysis of the CSF protein network was used to compare ALS and Parkinson’s disease protein co-correlation with healthy controls. In order to monitor temporal changes in the CSF proteome, we performed longitudinal analysis of the CSF proteome in a subset of ALS patients.ResultsWeighted correlation network analysis identified 10 modules, including those enriched for terms involved in gene expression including nucleic acid binding, RNA metabolism and translation; humoral immune system function, including complement pathways; membrane proteins, axonal outgrowth and adherence; and glutamatergic synapses. Immune system module eigenproteins were increased in ALS, whilst axonal module eigenproteins were decreased in ALS. The 19 altered protein correlations in ALS were enriched for gene expression (OR 3.05, p = 0.017) and membrane protein modules (OR 17.48, p = 0.011), including intramodular hub proteins previously identified as TDP-43 interactors. Proteins decreasing over longitudinal analysis ALS were enriched in glutamatergic synapse and axonal outgrowth modules. Protein correlation network disruptions in Parkinson’s disease showed no module enrichment.ConclusionsAlterations in the co-correlation network in CSF samples identified a set of pathways known to be associated with TDP-43 dysfunction in the pathogenesis of ALS, with important implications for therapeutic targeting and biomarker development.
Molecular Subtypes and CD4+ Memory T Cell-Based Signature Associated With Clinical Outcomes in Gastric Cancer
Authors: Ning Zhi-Kun , Hu Ce-Gui , Huang Chao , Liu Jiang , Zhou Tai-Cheng , Zong Zhen 
Published: March 2021

BackgroundCD4+ memory T cells are an important component of the tumor microenvironment (TME) and affect tumor occurrence and progression. Nevertheless, there has been no systematic analysis of the effect of CD4+ memory T cells in gastric cancer (GC).MethodsThree datasets obtained from microarray and the corresponding clinical data of GC patients were retrieved and downloaded from the Gene Expression Omnibus (GEO) database. We uploaded the normalize gene expression data with standard annotation to the CIBERSORT web portal for evaluating the proportion of immune cells in the GC samples. The WGCNA was performed to identify the modules the CD4+ memory T cell related module (CD4+ MTRM) which was most significantly associated with CD4+ memory T cell. Univariate Cox analysis was used to screen prognostic CD4+ memory T cell-related genes (CD4+ MTRGs) in CD4+ MTRM. LASSO analysis and multivariate Cox analysis were then performed to construct a prognostic gene signature whose effect was evaluated by Kaplan-Meier curves and receiver operating characteristic (ROC), Harrell’s concordance index (C-index), and decision curve analyses (DCA). A prognostic nomogram was finally established based on the CD4+ MTRGs.ResultWe observed that a high abundance of CD4+ memory T cells was associated with better survival in GC patients. CD4+ MTRM was used to stratify GC patients into three clusters by unsupervised clustering analysis and ten CD4+ MTRGs were identified. Overall survival, five immune checkpoint genes and 17 types of immunocytes were observed to be significantly different among the three clusters. A ten-CD4+ MTRG signature was constructed to predict GC patient prognosis. The ten-CD4+ MTRG signature could divide GC patients into high- and low-risk groups with distinct OS rates. Multivariate Cox analysis suggested that the ten-CD4+ MTRG signature was an independent risk factor in GC. A nomogram incorporating this signature and clinical variables was established, and the C-index was 0.73 (95% CI: 0.697–0.763). Calibration curves and DCA presented high credibility for the OS nomogram.ConclusionWe identified three molecule subtypes, ten CD4+ MTRGs, and generated a prognostic nomogram that reliably predicts OS in GC. These findings have implications for precise prognosis prediction and individualized targeted therapy.