bluuebunny/crossref_metadata_2025_split huggingface dataset:
Global Leadership in Paediatric and Congenital Cardiac Care: “Coding our way to improved care: an interview with Rodney C. G. Franklin, MBBS, MD, FRCP, FRCPCH”
Authors: Tretter Justin T. , Jacobs Jeffrey P. 
Published: February 2021

AbstractDr Rodney Franklin is the focus of our third in a planned series of interviews in Cardiology in the Young entitled, “Global Leadership in Paediatric and Congenital Cardiac Care.” Dr Franklin was born in London, England, spending the early part of his childhood in the United States of America before coming back to England. He then attended University College London Medical School and University College Hospital in London, England, graduating in 1979. Dr Franklin would then go on to complete his general and neonatal paediatrics training in 1983 at Northwick Park Hospital and University College Hospital in London, England, followed by completing his paediatric cardiology training in 1989 at Great Ormond Street Hospital for Children in London, England. During this training, he additionally would hold the position of British Heart Foundation Junior Research Fellow from 1987 to 1989. Dr Franklin would then complete his training in 1990 as a Senior Registrar and subsequent Consultant in Paediatric and Fetal Cardiology at Wilhelmina Sick Children’s Hospital in Utrecht, the Netherlands. He subsequently obtained his research doctorate at University of London in 1997, consisting of a retrospective audit of 428 infants with functionally univentricular hearts.Dr Franklin has spent his entire career as a Consultant Paediatric Cardiologist at the Royal Brompton & Harefield Hospital NHS Foundation Trust, being appointed in 1991. He additionally holds honorary Consultant Paediatric Cardiology positions at Hillingdon Hospital, Northwick Park Hospital, and Lister Hospital in the United Kingdom, and Honorary Senior Lecturer at Imperial College, London. He has been the Clinical Lead of the National Congenital Heart Disease Audit (2013–2020), which promotes data collection within specialist paediatric centres. Dr Franklin has been a leading figure in the efforts towards creating international, pan European, and national coding systems within the multidisciplinary field of congenital cardiac care. These initiatives include but are not limited to the development and maintenance of The International Paediatric & Congenital Cardiac Code and the related International Classification of Diseases 11th Revision for CHD and related acquired terms and definitions. This article presents our interview with Dr Franklin, an interview that covers his experience in developing these important coding systems and consensus nomenclature to both improve communication and the outcomes of patients. We additionally discuss his experience in the development and implementation of strategies to assess the quality of paediatric and congenital cardiac care and publicly report outcomes.
Applications of metamaterial sensors: a review
Authors: Prakash Divya , Gupta Nisha 
Published: February 2021

AbstractSensors based on metamaterial absorbers are very promising when it comes to high sensitivity and quality factor, cost, and ease of fabrication. The absorbers could be used to sense physical parameters such as temperature, pressure, density as well as they could be used for determining electromagnetic properties of materials and their characterization. In this work, an attempt has been made to explore the various possible applications of these sensors. Metamaterial-based sensors are very popular for its diverse applications in areas such as biomedical, chemical industry, food quality testing, agriculture. Split-ring resonators with various shapes and topologies are the most frequently used structures where the sensing principle is based on electromagnetic interaction of the material under test with the resonator. Overcoming the design challenges using metamaterial sensors involving several constraints such as cost, compactness, reusability, ease in fabrication, and robustness is also addressed.
A wide stopband multilayer substrate integrated waveguide bandpass filter with suppression of higher-order mode coupling
Authors: Tang Cong , Cheng Fei , Gu Chao 
Published: February 2021

AbstractThis paper presents a compact multilayer substrate integrated waveguide (SIW) bandpass filter with wide stopband. The square SIW cavity resonators in multilayer substrates are horizontally and vertically coupled with magnetic coupling. By properly designing the coupling structure, the couplings between the higher-order resonant modes can be suppressed. Compared with the conventional single-layer SIW filters, the proposed multilayer SIW filter also exhibits a compact size. To verify the design concept, a double-layered SIW filter is fabricated and measured. The measured results agree well with the simulations. The measured results show that the upper stopband of the filter is extended to 2.73 times of the center frequency 8.02 GHz.
Respiratory viral infections during pregnancy: effects of SARS-CoV-2 and other related viruses over the offspring
Authors: Riedel Claudia , Rivera Juan Carlos , Canedo-Marroquín Gisela , Kalergis Alexis M. , Opazo Ma. Cecilia 
Published: February 2021

AbstractLittle is known about the consequences of viral infection for pregnant woman or for the fetus. This issue became important with the appearance of the severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2). The infection with SARS-CoV-2 causes a respiratory syndrome known as COVID-19. The fast spreading around the world and the fact that without a treatment or vaccine humans are completely exposed, converts emerging viral diseases in a significant risk for pregnant women and their infants. At this time, during SARS-CoV-2 pandemics pregnant women are not considered as a risk population and little is known about the effects of viral infections over the offspring although the amount of emerging evidence showing detrimental effects for the mother and the fetus. This issue highlights the importance to understand the effects of viral infections during pregnancy. In this work, we analyze the effects of viral infections, like SARS-CoV-2 and other related viruses during pregnancy over the mother and the consequences for the offspring.
Fine Structure of the Breakthrough Phase of the Attachment Process in a Natural Lightning Flash
Authors: Jiang Rubin , Srivastava Abhay , Qie Xiushu , Yuan Shanfeng , Zhang Hongbo , Sun Zhuling , Wang Dongfang , Wang Caixia , Lv Guanlin , Li Zongxiang 
Published: February 2021

AbstractA lightning flash striking a 325‐m tower was captured using a high‐speed camera operated at 380 kfps and the characteristics of breakthrough phase (BTP) were recorded in two consecutive frames. The fine time‐resolved images indicate that the common streamer zone (CSZ) formed when the bright tips of negative and positive leaders were ≥23 m apart from each other. During BTP, both the negative and positive leaders extended their channels with enhanced luminosity and the gap between them was shortened to 16 m in the frame prior to return stroke. A space leader‐like luminous segment was found within the route of leader connection. This route is inferred to have already been determined at the initial formation of CSZ, by the interconnected streamers of approaching leaders. The dominant growth of this route is interpreted as a new scenario for the conversion of a high‐impedance CSZ to a hot plasma channel during BTP.
Association between methylation of BIN1 promoter in peripheral blood and preclinical Alzheimer’s disease
Authors: Hu Hao , Tan Lan , Bi Yan-Lin , Xu Wei , Tan Lin , Shen Xue-Ning , Hou Xiao-He , Ma Ya-Hui , Dong Qiang , Yu Jin-Tai 
Published: February 2021

AbstractThe bridging integrator 1 (BIN1) gene is the second most important susceptibility gene for late-onset Alzheimer’s disease (LOAD) after apolipoprotein E (APOE) gene. To explore whether the BIN1 methylation in peripheral blood changed in the early stage of LOAD, we included 814 participants (484 cognitively normal participants [CN] and 330 participants with subjective cognitive decline [SCD]) from the Chinese Alzheimer’s Biomarker and LifestylE (CABLE) database. Then we tested associations of methylation of BIN1 promoter in peripheral blood with the susceptibility for preclinical AD or early changes of cerebrospinal fluid (CSF) AD-related biomarkers. Results showed that SCD participants with significant AD biological characteristics had lower methylation levels of BIN1 promoter, even after correcting for covariates. Hypomethylation of BIN1 promoter were associated with decreased CSF Aβ42 (p = 0.0008), as well as increased p-tau/Aβ42 (p = 0.0001) and t-tau/Aβ42 (p < 0.0001) in total participants. Subgroup analysis showed that the above associations only remained in the SCD subgroup. In addition, hypomethylation of BIN1 promoter was also accompanied by increased CSF p-tau (p = 0.0028) and t-tau (p = 0.0130) in the SCD subgroup, which was independent of CSF Aβ42. Finally, above associations were still significant after correcting single nucleotide polymorphic sites (SNPs) and interaction of APOE ɛ4 status. Our study is the first to find a robust association between hypomethylation of BIN1 promoter in peripheral blood and preclinical AD. This provides new evidence for the involvement of BIN1 in AD, and may contribute to the discovery of new therapeutic targets for AD.
Host immunity modulates the efficacy of microbiota transplantation for treatment of Clostridioides difficile infection
Authors: Littmann Eric R. , Lee Jung-Jin , Denny Joshua E. , Alam Zahidul , Maslanka Jeffrey R. , Zarin Isma , Matsuda Rina , Carter Rebecca A. , Susac Bože , Saffern Miriam S. , Fett Bryton , Mattei Lisa M. , Bittinger Kyle , Abt Michael C. 
Published: February 2021

AbstractFecal microbiota transplantation (FMT) is a successful therapeutic strategy for treating recurrent Clostridioides difficile infection. Despite remarkable efficacy, implementation of FMT therapy is limited and the mechanism of action remains poorly understood. Here, we demonstrate a critical role for the immune system in supporting FMT using a murine C. difficile infection system. Following FMT, Rag1 heterozygote mice resolve C. difficile while littermate Rag1−/− mice fail to clear the infection. Targeted ablation of adaptive immune cell subsets reveal a necessary role for CD4+ Foxp3+ T-regulatory cells, but not B cells or CD8+ T cells, in FMT-mediated resolution of C. difficile infection. FMT non-responsive mice exhibit exacerbated inflammation, impaired engraftment of the FMT bacterial community and failed restoration of commensal bacteria-derived secondary bile acid metabolites in the large intestine. These data demonstrate that the host’s inflammatory immune status can limit the efficacy of microbiota-based therapeutics to treat C. difficile infection.
Extreme temperatures compromise male and female fertility in a large desert bird
Authors: Schou Mads F. , Bonato Maud , Engelbrecht Anel , Brand Zanell , Svensson Erik I. , Melgar Julian , Muvhali Pfunzo T. , Cloete Schalk W. P. , Cornwallis Charlie K. 
Published: February 2021

AbstractTemperature has a crucial influence on the places where species can survive and reproduce. Past research has primarily focused on survival, making it unclear if temperature fluctuations constrain reproductive success, and if so whether populations harbour the potential to respond to climatic shifts. Here, using two decades of data from a large experimental breeding programme of the iconic ostrich (Struthio camelus) in South Africa, we show that the number of eggs females laid and the number of sperm males produced were highly sensitive to natural temperature extremes (ranging from −5 °C to 45 °C). This resulted in reductions in reproductive success of up to 44% with 5 °C deviations from their thermal optimum. In contrast, gamete quality was largely unaffected by temperature. Extreme temperatures also did not expose trade-offs between gametic traits. Instead, some females appeared to invest more in reproducing at high temperatures, which may facilitate responses to climate change. These results show that the robustness of fertility to temperature fluctuations, and not just temperature increases, is a critical aspect of species persistence in regions predicted to undergo the greatest change in climate volatility.
Genomic evidence of prevalent hybridization throughout the evolutionary history of the fig-wasp pollination mutualism
Authors: Wang Gang , Zhang Xingtan , Herre Edward Allen , McKey Doyle , Machado Carlos A. , Yu Wen-Bin , Cannon Charles H. , Arnold Michael L. , Pereira Rodrigo A. S. , Ming Ray , Liu Yi-Fei , Wang Yibin , Ma Dongna , Chen Jin 
Published: February 2021

AbstractFicus(figs) and their agaonid wasp pollinators present an ecologically important mutualism that also provides a rich comparative system for studying functional co-diversification throughout its coevolutionary history (~75 million years). We obtained entire nuclear, mitochondrial, and chloroplast genomes for 15 species representing all major clades ofFicus. Multiple analyses of these genomic data suggest that hybridization events have occurred throughoutFicusevolutionary history. Furthermore, cophylogenetic reconciliation analyses detect significant incongruence among all nuclear, chloroplast, and mitochondrial-based phylogenies, none of which correspond with any published phylogenies of the associated pollinator wasps. These findings are most consistent with frequent host-switching by the pollinators, leading to fig hybridization, even between distantly related clades. Here, we suggest that these pollinator host-switches and fig hybridization events are a dominant feature of fig/wasp coevolutionary history, and by generating novel genomic combinations in the figs have likely contributed to the remarkable diversity exhibited by this mutualism.
Plasma membrane H+-ATPase overexpression increases rice yield via simultaneous enhancement of nutrient uptake and photosynthesis
Authors: Zhang Maoxing , Wang Yin , Chen Xi , Xu Feiyun , Ding Ming , Ye Wenxiu , Kawai Yuya , Toda Yosuke , Hayashi Yuki , Suzuki Takamasa , Zeng Houqing , Xiao Liang , Xiao Xin , Xu Jin , Guo Shiwei , Yan Feng , Shen Qirong , Xu Guohua , Kinoshita Toshinori , Zhu Yiyong 
Published: February 2021

AbstractNitrogen (N) and carbon (C) are essential elements for plant growth and crop yield. Thus, improved N and C utilisation contributes to agricultural productivity and reduces the need for fertilisation. In the present study, we find that overexpression of a single rice gene, Oryza sativa plasma membrane (PM) H+-ATPase 1 (OSA1), facilitates ammonium absorption and assimilation in roots and enhanced light-induced stomatal opening with higher photosynthesis rate in leaves. As a result, OSA1 overexpression in rice plants causes a 33% increase in grain yield and a 46% increase in N use efficiency overall. As PM H+-ATPase is highly conserved in plants, these findings indicate that the manipulation of PM H+-ATPase could cooperatively improve N and C utilisation, potentially providing a vital tool for food security and sustainable agriculture.
Intelligent driving intelligence test for autonomous vehicles with naturalistic and adversarial environment
Authors: Feng Shuo , Yan Xintao , Sun Haowei , Feng Yiheng , Liu Henry X. 
Published: February 2021

AbstractDriving intelligence tests are critical to the development and deployment of autonomous vehicles. The prevailing approach tests autonomous vehicles in life-like simulations of the naturalistic driving environment. However, due to the high dimensionality of the environment and the rareness of safety-critical events, hundreds of millions of miles would be required to demonstrate the safety performance of autonomous vehicles, which is severely inefficient. We discover that sparse but adversarial adjustments to the naturalistic driving environment, resulting in the naturalistic and adversarial driving environment, can significantly reduce the required test miles without loss of evaluation unbiasedness. By training the background vehicles to learn when to execute what adversarial maneuver, the proposed environment becomes an intelligent environment for driving intelligence testing. We demonstrate the effectiveness of the proposed environment in a highway-driving simulation. Comparing with the naturalistic driving environment, the proposed environment can accelerate the evaluation process by multiple orders of magnitude.
Nucleic acid binding by SAMHD1 contributes to the antiretroviral activity and is enhanced by the GpsN modification
Authors: Yu Corey H. , Bhattacharya Akash , Persaud Mirjana , Taylor Alexander B. , Wang Zhonghua , Bulnes-Ramos Angel , Xu Joella , Selyutina Anastasia , Martinez-Lopez Alicia , Cano Kristin , Demeler Borries , Kim Baek , Hardies Stephen C. , Diaz-Griffero Felipe , Ivanov Dmitri N. 
Published: February 2021

AbstractSAMHD1 impedes infection of myeloid cells and resting T lymphocytes by retroviruses, and the enzymatic activity of the protein—dephosphorylation of deoxynucleotide triphosphates (dNTPs)—implicates enzymatic dNTP depletion in innate antiviral immunity. Here we show that the allosteric binding sites of the enzyme are plastic and can accommodate oligonucleotides in place of the allosteric activators, GTP and dNTP. SAMHD1 displays a preference for oligonucleotides containing phosphorothioate bonds in the Rp configuration located 3’ to G nucleotides (GpsN), the modification pattern that occurs in a mechanism of antiviral defense in prokaryotes. In the presence of GTP and dNTPs, binding of GpsN-containing oligonucleotides promotes formation of a distinct tetramer with mixed occupancy of the allosteric sites. Mutations that impair formation of the mixed-occupancy complex abolish the antiretroviral activity of SAMHD1, but not its ability to deplete dNTPs. The findings link nucleic acid binding to the antiretroviral activity of SAMHD1, shed light on the immunomodulatory effects of synthetic phosphorothioated oligonucleotides and raise questions about the role of nucleic acid phosphorothioation in human innate immunity.
Cryo-EM of mammalian PA28αβ-iCP immunoproteasome reveals a distinct mechanism of proteasome activation by PA28αβ
Authors: Chen Jinhuan , Wang Yifan , Xu Cong , Chen Kaijian , Zhao Qiaoyu , Wang Shutian , Yin Yue , Peng Chao , Ding Zhanyu , Cong Yao 
Published: February 2021

AbstractThe proteasome activator PA28αβ affects MHC class I antigen presentation by associating with immunoproteasome core particles (iCPs). However, due to the lack of a mammalian PA28αβ-iCP structure, how PA28αβ regulates proteasome remains elusive. Here we present the complete architectures of the mammalian PA28αβ-iCP immunoproteasome and free iCP at near atomic-resolution by cryo-EM, and determine the spatial arrangement between PA28αβ and iCP through XL-MS. Our structures reveal a slight leaning of PA28αβ towards the α3-α4 side of iCP, disturbing the allosteric network of the gatekeeper α2/3/4 subunits, resulting in a partial open iCP gate. We find that the binding and activation mechanism of iCP by PA28αβ is distinct from those of constitutive CP by the homoheptameric TbPA26 or PfPA28. Our study sheds lights on the mechanism of enzymatic activity stimulation of immunoproteasome and suggests that PA28αβ-iCP has experienced profound remodeling during evolution to achieve its current level of function in immune response.
Flux periodic oscillations and phase-coherent transport in GeTe nanowire-based devices
Authors: Zhang Jinzhong , Tse Pok-Lam , Jalil Abdur-Rehman , Kölzer Jonas , Rosenbach Daniel , Luysberg Martina , Panaitov Gregory , Lüth Hans , Hu Zhigao , Grützmacher Detlev , Lu Jia Grace , Schäpers Thomas 
Published: February 2021

AbstractDespite the fact that GeTe is known to be a very interesting material for applications in thermoelectrics and for phase-change memories, the knowledge on its low-temperature transport properties is only limited. We report on phase-coherent phenomena in the magnetotransport of GeTe nanowires. From universal conductance fluctuations measured on GeTe nanowires with Au contacts, a phase-coherence length of about 280 nm at 0.5 K is determined. The distinct phase-coherence is confirmed by the observation of Aharonov–Bohm type oscillations for parallel magnetic fields. We interpret the occurrence of these magnetic flux-periodic oscillations by the formation of a tubular hole accumulation layer. For Nb/GeTe-nanowire/Nb Josephson junctions we obtained a critical current of 0.2 μA at 0.4 K. By applying a perpendicular magnetic field the critical current decreases monotonously with increasing field, whereas in a parallel field the critical current oscillates with a period of the magnetic flux quantum confirming the presence of a tubular hole channel.
Activatable polymer nanoagonist for second near-infrared photothermal immunotherapy of cancer
Authors: Jiang Yuyan , Huang Jiaguo , Xu Cheng , Pu Kanyi 
Published: February 2021

AbstractNanomedicine in combination with immunotherapy offers opportunities to treat cancer in a safe and effective manner; however, remote control of immune response with spatiotemporal precision remains challenging. We herein report a photothermally activatable polymeric pro-nanoagonist (APNA) that is specifically regulated by deep-tissue-penetrating second near-infrared (NIR-II) light for combinational photothermal immunotherapy. APNA is constructed from covalent conjugation of an immunostimulant onto a NIR-II semiconducting transducer through a labile thermo-responsive linker. Upon NIR-II photoirradiation, APNA mediates photothermal effect, which not only triggers tumor ablation and immunogenic cell death but also initiates the cleavage of thermolabile linker to liberate caged agonist for in-situ immune activation in deep solid tumor (8 mm). Such controlled immune regulation potentiates systemic antitumor immunity, leading to promoted cytotoxic T lymphocytes and helper T cell infiltration in distal tumor, lung and liver to inhibit cancer metastasis. Thereby, the present work illustrates a generic strategy to prepare pro-immunostimulants for spatiotemporal regulation of cancer nano-immunotherapy.
QM7-X, a comprehensive dataset of quantum-mechanical properties spanning the chemical space of small organic molecules
Authors: Hoja Johannes , Medrano Sandonas Leonardo , Ernst Brian G. , Vazquez-Mayagoitia Alvaro , DiStasio Robert A. , Tkatchenko Alexandre 
Published: February 2021

AbstractWe introduce QM7-X, a comprehensive dataset of 42 physicochemical properties for ≈4.2 million equilibrium and non-equilibrium structures of small organic molecules with up to seven non-hydrogen (C, N, O, S, Cl) atoms. To span this fundamentally important region of chemical compound space (CCS), QM7-X includes an exhaustive sampling of (meta-)stable equilibrium structures—comprised of constitutional/structural isomers and stereoisomers, e.g., enantiomers and diastereomers (including cis-/trans- and conformational isomers)—as well as 100 non-equilibrium structural variations thereof to reach a total of ≈4.2 million molecular structures. Computed at the tightly converged quantum-mechanical PBE0+MBD level of theory, QM7-X contains global (molecular) and local (atom-in-a-molecule) properties ranging from ground state quantities (such as atomization energies and dipole moments) to response quantities (such as polarizability tensors and dispersion coefficients). By providing a systematic, extensive, and tightly-converged dataset of quantum-mechanically computed physicochemical properties, we expect that QM7-X will play a critical role in the development of next-generation machine-learning based models for exploring greater swaths of CCS and performing in silico design of molecules with targeted properties.