bluuebunny/crossref_metadata_2025_split huggingface dataset:
Timosaponin Aiii Disrupts Morphological Plasticity and Migration of Breast Adenocarcinoma Through Inhibition of Integrin Internalisation
Authors: Terabayashi Takeshi , Hanada Katsuhiro , Matsuoka Shigeru , Sasaki Takako , Katoh Akira , Akamine Takahiro , Ishizaki Toshimasa 
Published: March 2021

Abstract
Cell migration is a critical step in tumour invasion and metastasis. To acquire invasive properties, cancer cells use their surrounding environment through dynamic and bidirectional interactions and change their morphology and mode of migration. Thus, inhibition of morphological plasticity regulated by paracrine interactions may be a promising approach for anti-cancer therapy. In this study, we found that timosaponin AIII (TAIII), a steroidal saponin isolated from the roots of Anemarrhena asphodeloides, disrupted the morphological changes and migratory activity of breast adenocarcinoma cells promoted by paracrine interactions with mammary epithelium-derived cells. TAIII suppressed lamellipodia formation of MDA-MB-231 cells in response to exogenous stimuli from MCF10A cells, thereby inhibiting morphological changes and migration. TAIII also attenuated membrane spreading and induced contraction of HeLa cells, followed by expansion of intercellular gaps. Furthermore, we analysed the intracellular dynamics of TAIII labelled with a fluorescent dye and found that labelled TAIII was internalised in a manner dependent on dynamin. We also found that TAIII blocked internalisation of cell surface proteins including integrin b1. These results provide a novel aspect to understand how TAIII exerts pharmacological activities in suppression of cancer cell migration.
CRISPR/Cas9-based Knockout of miR-487b-3p Accelerates The Cell Proliferation of Primary Goat Myoblast by Relieving Its Inhibition of IRS1
Authors: Lyu Ming , Wang Xu , Qian Hongrun , Ma Jinrong , Sun Yongsen , Meng Xiangyu , Zhang Wenqiang , Zhang Zhiying , Xu Kun 
Published: March 2021

Abstract
Background: MicroRNAs (miRNAs) are endogenously expressed small noncoding RNAs with a length of about 22 nucleotides and play critical roles in the regulation of posttranscriptional gene expression. miR-487b-3p has been recently identified as a key regulator in skeletal muscle growth and development. However, the function of miR-487b-3p on goat skeletal muscle remains to be investigated.Results: In this study, we found that miR-487b-3p was widespread in goat different tissues, with a significantly higher expression in muscle, especially in lamb. The results demonstrated that the expression of miR-487b-3p was gradually up-regulated during myoblast proliferation. Then, miR-487b-3p knockout primary goat myoblast cells clones were obtained by using CRISPR/Cas9 system and our RPG surrogate reporter-based screening. Further investigation uncovered that the knockout of miR-487b-3p significantly accelerated primary goat myoblast proliferation, which was accompanied by up-regulation of cell cycle-related genes, such as PCNA, Cyclin E and CDK2. What’s more, we found that miR-487b-3p targets directly the 3'UTR of insulin receptor substrate 1 (IRS1) gene and IRS1 knockdown by siRNA was able to down-regulate the expression of the cell cycle-related genes.Conclusions: Collectively, these founding demonstrated miR-487b-3p as a potent inhibitor of cell proliferation which functions by targeting IRS1 gene in primary goat myoblast.
Evaluation of Lablab Purpureus (L.) Sweet Germplasm Using Yield and Quality Traits
Authors: S VISHNU V , RADHAMANY P.M. 
Published: March 2021

Abstract
The crops that are not commercially grown or widely traded are known as underutilized or unusable crops. Most of the legumes have come under these categories. Giving importance to such neglected and underutilized crops is an effective way to maintain a diverse and healthy diet and to combat micronutrient deficiencies. Lablab purpureus (L.) Sweet is one of the most important underutilized legume vegetable crops, which exhibits broad genetic variability in multiple characteristics such as the habit, pigmentation of the stem, and color of the pod. In the past, researchers paid very little attention to the systematic crop improvement of L.purpureus. In the present study, fifty accessions of L. purpureus were collected from different places of Kerala and Tamil Nadu and analyzed for yield, yield contributing characters, and biochemical factors. Nutritional and anti-nutritional factors were analyzed using the standard protocol. The accessions exhibited significant variations for all the characters tested. The nutritional content of all the accessions were found to be high, whereas anti-nutritional factors were very low. The differences in phenotypic and genotypic coefficient of variation was compared for different traits, and it was found to be very narrow for almost all the characters studied. The fifty genotypes involved in the study varied in the number of pod/ inflorescence, length and width of the pod, number of flower/ inflorescence, and length of the peduncle. So these observations can be used as a baseline data for the selection of parental lines for future improvement of Lablab purpureus.
Laser-Assisted 3D Printing of Carbon Fibre Reinforced Plastic Parts With Sandwiching Fibres Between Plastic Layers
Authors: Nakagawa Yuki , Mori Ken-ichiro , Yoshino Masahiko 
Published: March 2021

Abstract
A laser-assisted 3D printing process of carbon fibre reinforced plastic parts with sandwiching fibres between plastic layers was developed to improve the bond strength of the fibres to the plastic layers. In this process, the bunded carbon fibres were placed on the 3D-printed lower layer, then the upper plastic layer was deposited on the fibres, and the two layers with sandwiching the fibres were laser-heated. The heating temperature at the interface between the fibres and the plastic layer was changed by the colour of the plastic layer because of the transmittance and absorption of laser beam, and the translucent layer was most appropriate. Not only the strength but also the rigidity of the 3D-printed carbon fibre reinforced plastic part was improved by laser heating. Carbon fibre reinforced plastic parts having closed cross-section was manufactured, and strengthened by optimisation sandwiched fibre orientation. A tailored part locally reinforced by carbon fibres was 3D-printed.
Flaxseed Oil Attenuates Trimethyltin-induced Neurodegeneration via Down-regulation of Inflammatory Activity of Astrocytes
Authors: Mitrović Nataša , Dragić Milorad , Zarić Marina , Nedeljković Nadežda , Grković Ivana 
Published: March 2021

Abstract
Trimethyltin chloride (TMT) is an organotin neurotoxicant that selectively targets the hippocampus, and induces selective and progressive neuronal loss, gliosis, neuroinflammation and cognitive deficits, thus resembling critical features of Alzheimer’s diseases (AD). Flaxseed oil (FSO) is anti-inflammatory agent with potent neuroprotective properties. Therefore, the presented study was designed to evaluate the protective effects of flaxseed oil (FSO) continuous pretreatment to alleviate TMT- (8 mg/kg) induced neurodegeneration. Ovariectomized (OVX) female rats were pretreated with FSO (1 ml/kg, orally) for two weeks. At day 14, part of animals received single dose of TMT (8 mg/kg, i.p.) and application of FSO continued for seven more days. Data have convincingly shown that FSO counteracted TMT effects. Specifically, daily administration of FSO improved TMT- induced behavioral manifestations manifested as hyper-excitability, and hyper-responsiveness, reduced neuronal loss, ameliorated expression of pro-inflammatory cytokines (tumor necrosis factor (TNF)-α, interleukin (IL)-10, and IL-6), alleviated astrogliosis and A1-like astrocytes conversion which was related to down-regulation of aromatase/estrogen receptor α signaling, and microgliosis. Together, these findings support beneficial neuroprotective properties of FSO against TMT-induced neurotoxicity and hint at a promising preventive use of FSO in hippocampal degeneration and dysfunction.
Combining Molecular Docking and Graph Convolutional Network to Discover Potential Selective Limk1 Inhibitors
Authors: Zhong Weihe , Zhao Lu , Chen Calvin Yu-Chian 
Published: March 2021

Abstract
The LIM kinase 1 (Limk1) has been demonstrated to be considered a therapeutic target and selective inhibitors of Limk1 rather Rho-associated kinase 2 (ROCK2) are considered of interest for the treatment of several indications such as Alzheimer’s disease (AD), Parkinson’s disease (PD) and cancer migration/invasion. Here, we utilized molecular docking to screen potential compounds of Limk1 from Traditional Chinese Medicine (TCM) database. Meanwhile, we performed a three-dimensional graph convolutional network (3DGCN), based on 3D molecular graph, to predict the inhibitory activity of Limk1 and ROCK2. Compared with the baseline models (RF, GCN and Weave), the 3DGCN achieved higher accuracy and the averaged RMSE values on test sets for Limk1 and ROCK2 were 0.721 and 0.852 respectively. In 3DGCN, above 80% of the test-set molecules from both two datasets were predicted within absolute error of 1.0 and the feature visualization suggested that it could automatically learn relevant structure features including 3D molecular information from a specific task for prediction. Furthermore, molecular dynamics (MD) simulations within 100 ns were employed to verify the stability of ligand-protein complexes and reveal the binding modes of the potential selective lead compounds of Limk1. Finally, integrating docking results, the predicted values by the 3DGCN and the MD analysis, we found that 7549, 2007_15649 and 3519 might be the potential and selective inhibitors for Limk1 receptor rather than ROCK2.
Expression of Glucocorticoid Receptors in the Early Period After the Return of Spontaneous Circulation Among Patients Who Experienced Cardiac Arrest : A Retrospective Observational Study
Authors: Yu Yanan , Tang Ziren , Li Jiabao , Xie Miaorong , Hang Chenchen , An Le , Li Chunsheng 
Published: March 2021

Abstract
Background: Rapid changes in glucocorticoid (GC) levels and adrenal insufficiency are related to the development of post-cardiac arrest syndrome. However, changes in glucocorticoid receptors (GR) have not been studied. Hence, this study aimed to investigate the association of early changes in GR and prognosis and immune response in patients who experienced cardiac arrest (CA). Methods: In this observational single-center case-control study, we enrolled patients who were in the early period of return of spontaneous circulation after CA and were admitted to the emergency department of the Beijing Chaoyang Hospital between October 2018 and October 2019. Age- and sex-matched healthy individuals were recruited for the control group after a physical examination.GR expression and cell counts of circulatory T and B lymphocytes, natural killer, and regulatory T (Treg) cells were assessed. Plasma total cortisol and adrenocorticotrophic hormone (ACTH) levels were tested. Since the data for total cortisol and ACTH levels had a skewed distribution, we compared our results with the natural logarithmic conversion values after adding 1 (ln [total cortisol+ 1], ln [ACTH+ 1]). Measurement data with a skewed distribution are expressed as medians (25th and 75th percentiles). The Mann–Whitney U test was used to compare variables between groups. The qualitative parameters in the 2 × 2 contingency table were used for analysis.Results: Overall, 85 patients who experienced CA and 40 healthy individuals were enrolled. All cell counts were lower and plasma total cortisol levels were higher (P<0.001) in patients who experienced CA than those in the healthy control group. GR expression in Treg cells and CD3+CD4+ T lymphocytes was not significantly different, but the mean fluorescence intensity and GR expression in other cells were lower in patients who experienced CA (P<0.05) than those in the healthy control group. ACTH levels did not show any difference. There were no significant differences between survivors and non-survivors. Conclusion: Our findings provide insights into GC sensitivity and immunosuppressive status in these patients, and a new perspective for GC targeted treatment.
Effectiveness of Single Dose Premedication of Piroxicam and Prednisolone on Post Endodontic Pain in One Visit Root Canal Treatment: A Randomized Trial
Authors: TANWIR ANUM , AHMED SHAHBAZ , AKHTAR HIRA , WAHID UMAIR , SHAKOOR MARIA , SHAHNAWAZ DANISH 
Published: March 2021

Abstract
BACKGROUNDMany approaches have been investigated for pain relief after root canal procedure. In this study we compare the effectiveness of premedication drugs, given single dose Piroxicam and Prednisolone separately to evaluate post endodontic pain at different time intervals (24, 48, 72 and 96 hours) using visual analog scale (VAS) after single visit root canal treatment.METHODSTotal number of 120 patients identified with symptomatic irreversible pulpitis were made part of this research after signing informed consent. The pain intensity levels were marked through the use of VAS scale before the commencement of treatment. The participants were randomly placed in three groups (n=40) Group I: Control, Group II: Piroxicam (20 mg) and Group III: Prednisolone (20 mg). The drugs were administered thirty minutes before endodontic procedure was initiated. Root canal procedure was carried out followed by placement of provisional restoration on a single appointment. The patients were advised to continue marking their pain intensity levels after 24, 48, 72 and 96 hours using VAS. All patients were called for follow up after 4 days for clinical evaluation and placement of permanent restoration. The effectiveness of each drug over different time interval was studied employing repeated measure ANOVA. The significance level was considered p-value <0.05.RESULTSAdministration of pre-medication drug therapy with Piroxicam and Prednisolone was able to successfully alleviate post-endodontic pain. However, the long term effectiveness (96 hours) of both drugs to reduce post-endodontic pain was observed to be statistically insignificant.CONCLUSIONPre-medication with either single dose Piroxicam and Prednisolone was found to be effective against post-endodontic pain, in patients presenting with symptomatic irreversible pulpitis.TRIAL REGISTRATION: This single blinded, randomized clinical trial [registration no. NCT04124822(11/10/2019)] was performed in Operative Dentistry department of Dr. Ishrat-ul-Ebad Khan Institute of Oral Health Sciences.
Association Between Social Capital and Performance Among Core Members of Chinese Ngos in Aids Prevention and Control: A Cross-sectional Study
Authors: Sang Lingzhi , Liu Meng , Xu Xiaoru , Bai Zhongliang , Zhang Xingxi , Wang Danni , Chen Ren 
Published: March 2021

Abstract
Background The role of non-governmental organizations in the field of AIDS prevention and control is increasingly being valued in China and even the world.This research aimed to study the status of social capital and performance of core members of non-governmental organizations (NGOs), as well as the relation between the two, in the field of HIV/AIDS prevention and control in China. Methods We used a multi-stage stratified cluster random sampling method to conduct a survey among 327 core members of 212 NGOs in the field of HIV prevention and control in eight provinces in China from July to December 2015. The questionnaire included items on demographic characteristics of core members, a social capital scale, and an organizational performance scale. We analyzed the data using chi-squared test and logistic regression.Results From the perspective of social capital, the core personnel of NGOs with extensive social networks and high levels of social support accounted for a relatively low proportion of the total sample, at 29.1% (95 people) and 36.7% (120 people), respectively. The chi-squared test results indicated the statistical significance of differences in factors such as educational background of the core personnel. The logistic regression analysis results indicated that trust in social capital was positively correlated with structural performance (OR = 2.21, 95% CI: 1.24–3.95). Social support, cohesion, and sense of belonging were correlated to the level of personal authority in performance evaluation (OR = 2.78, 95% CI: 1.43–5.39). The results also showed no statistically significant correlation between social network in social capital and performance level.Conclusions Improvement in social capital can promote better organizational performance. For the core members of NGOs, improvement in the level of social capital and performance was conducive to playing an active role in HIV/AIDS prevention and control. Our results can provide insights for decision-making and guiding the activities of NGOs engaged in HIV/AIDS prevention and control services.
Effect of postpartum experience and infant temperament on maternal beliefs about infant crying
Authors: Hiraoka Daiki , Nomura Michio , Kato Masaharu 
Published: March 2021

Abstract
Infant crying is an important signal for their survival and socio-cognitive development. It is unclear how maternal beliefs about infant crying change with their caregiving experience. Additionally, this study examined a bidirectional relationship between maternal beliefs and infants’ temperament using longitudinal study design. Maternal beliefs about crying and infant temperament of first-time mothers were measured at 1-month intervals over a 4-month period (N = 339 at Wave 1 and N = 164 at Wave 4). We found that parent-oriented beliefs about crying increased with the mother’s experience, and that infants high on surgency predicted changes in maternal beliefs. The current results suggest that the maternal beliefs change with an infant’s development and parenting experience in the early postpartum period.
Pattern and Distribution of Human Brucellosis Diagnosed in the National Animal and Plant Health Laboratory of Eritrea
Authors: Ghebrenegus Amon Solomon , Mengesha Soliana Binega , Abdulkadir Munir Wehab , Weldeyesus Mussie , Tewelde Asmerom Tesfagiorgis , Ghebrenegus Azariel Solomon 
Published: March 2021

Abstract
Background: Currently, brucellosis is the most widespread zoonotic disease in the world. In Eritrea, the disease has been documented since the 1940s, with widespread infections throughout the country.That being said, the only facility that tested for brucellosis is the National Animal and Plant Health Laboratory of Eritrea (NAPHL). Therefore, this study will focus on the pattern and distribution of human brucellosis tested and diagnosed at NAPHL. Method: This study is a retrospective cross-sectional look at all the Rose-Bengal test confirmed Brucellosis cases at the NAPHL from January, 2017 to December, 2019. Results:A total of 848 cases were detected out of 2,669 patients tested. The male to female ratio was 2.78 in those who tested positive for brucellosis. In the years, 2018 and 2019, the age range was between 1 and 89 with a mean of 29 years. Patients came from 5 of the 6 zones of Eritrea. Across the study period, no tests were recorded from the Southern Red Sea zone. Overall, Maekel Zone had the most cases, followed by Debub Zone and Northern Red Sea Zone.Scatter plot imaging by village showed a dense scatter area around the Maekel zone. Cases mapping by sub-zone showed highest number of cases in the Sub-Zones of the Maekel Zone and those located closest to the zone (namely Ghindae, Segheneiti, Dubarwa and Dekemhare). Conclusion:As human brucellosis is widespread in Eritrea,the government should take quick actions to increase accessibility to testing and treatment facilities,and implement an eradication program.
WITHDRAWN: Identification, Molecular Profiling, Docking Studies and determination of In-vivo Anti-Inflammatory Potential of Teucrium Stocksianum Bioss Fixed Oil (FO)
Authors: Shah Syed Muhammad Mukarram , Shah Syed Muhammad Hassan , Zafar Rehman , Khan Mohammad Ijaz , Hussain Fida , Zeb Anwar , Shah Syed Wadood Ali , Jan Muhammad Saeed 
Published: March 2021

Abstract
Background: Considering the positive and valuable upshots of the naturally occurring ingredients as the complementary treatment for many ailments, this study investigates the Teucrium Stocksianum Bioss Fixed Oil (FO) for the treatment of inflammation.Methods: Different plants of the Teucrium genera have been employed occasionally for the management of different disorders. Teucrium stocksianum (Lamiaceae) is traditionally use as antipyretic along with remedy of inflammation, diabetes and tumors. It also occupies the capacity to purify the blood. The aim of this study is to determine the chemical constituents and anti-inflammatory potentials of Teucrium stocksianum fixed oil (FO). Qualitative and quantitative analysis of fixed oil were performed via GC-MS (Gas Chromatography coupled with Mass Spectrometer). Preliminary inflammation antagonistic activity was calculated in mice using the carrageenan-induced paw edema model while the mechanism behind this inflammatory antagonistic effect was determined by employing various inflammogens including arachidonic acid, prostaglandins E2 and leukotriene via paw edema model.Results: The acute toxicity of fixed oil was determined at 3, 6 and 10 ml/kg body weight of the mouse. The GC-MS analysis documented 21 various unsaturated and saturated fatty acids elements. The methyl esters of octadecadienoic and linoleic acids were found predominantly at 22.60% and 23.84% respectively. In preliminary screening, FO demonstrated substantial anti-inflammatory capacity (67.87%, **P ˂ 0.01) in carrageenan induced paw edema model at 3 ml/kg body weight. The activity reached a peak value at the 3rd hour and continued persistent until the 5th hour of sample administration. FO displayed dose-dependent inhibition against all inflammogens at 3 ml/kg at 3rd h of the FO administration. These have shown marvelous protection i.e. 64.92%, 68.75%and 58.3% against arachidonic acid, prostaglandin E2 and leukotriene accordingly, at 3 ml/kg. The arachidonic acid antagonist (Caffeic acid) demonstrated 69.43% activity at 100 mg/kg after 3rd h of its administration while in the acute toxicity test, FO has shown no grass lethality at 10 ml/kg dose.Conclusion: According to our findings, the FO of T. stocksianum exhibits inflammation antagonistic potential through both lipoxygenase (LOX) and cyclooxygenase (COX) enzyme inhibition strategies that intensely sustenance the traditional practice of T. stocksianum in the management of multiple pathological inflammatory situations. The computed binding energies of the molecules exposed have synergistic potential to avert inflammation.