bluuebunny/crossref_metadata_2025_split huggingface dataset:
The effectiveness of cinnamon powder and cinnamon leaf extract to prevent Aeromonas hydrophila infection on striped catfish Pangasianodon hypophthalamus
Authors: Susanti Erni , Wahjuningrum Dinamella , Nuryati Sri , Setiawati Mia 
Published: October 2021

Striped catfish Pangasianodon hypophthalamus is one of the intensive cultured commodities. Disease outbreak becomes inevitable to prevent in a fish culture. One of the most frequent disease occurred in striped catfish is the motile aeromonas septicemia (MAS) disease caused by Aeromonas hydrophila. This study aimed to evaluate the effectiveness of 1% dietary cinnamon powder and 0.5% dietary cinnamon leaf extract on the immune response of striped catfish challenged with A. hydrophila. Striped catfish used in this study sizing of 5.80 ± 0.21 g. This study contained two phases, namely in vitro and in vivo tests. In vitro test contained inhibition zone and antibacterial tests, which demonstrates that 1% cinnamon powder and 0.5% cinnamon leaf extract are effective to inhibit A. hydrophila activity. In vivo test contained four treatments, i.e fish fed with 1% cinnamon leaf powder supplemented diet; 0.5% cinnamon leaf extract supplemented diet, positive control diet, and negative control diet. Each treatment was performed in three replications. The result showed that 1% cinnamon leaf powder supplemented diet obtained the best results to enhance the immune response of striped catfish higher survival rate value at 83.33% than the positive control diet (P<0.05).
Keywords: Aeromonas hydrophila, Cinnamomum burmannii, extract, Pangasianodon hypophthalmus, powder.
ABSTRAK
Ikan patin Pangasianodon hypophthalamus termasuk komoditas yang banyak dibudidayakan secara intensif. Kendala budidaya seperti penyakit pun sulit untuk dihindari. Salah satu jenis penyakit yang kerap menyerang ikan patin yaitu penyakit MAS (motile aermomonad septicaemia) yang disebabkan oleh Aeromonas hydrophila. Penelitian ini bertujuan untuk menguji efektivitas 1% (w/w) tepung dan 0,5% (w/w) ekstrak daun kayu manis dalam pakan sebagai upaya pencegahan infeksi bakteri A. hydrophila pada ikan patin. Ikan patin yang digunakan berukuran 5,80 ± 0,21 g. Penelitian ini terdiri dua tahap yaitu uji in vitro dan uji in vivo. Hasil uji in vitro terhadap aktivitas antibakteri menunjukkan bahwa dosis 1% (w/w) tepung daun kayu manis dan 0.5% (w/w) ekstrak daun kayu manis efektif dalam menghambat pertumbuhan A. hydrophila. Uji in vivo terdiri atas empat perlakuan yaitu pemberian pakan dengan penambahan tepung daun kayu manis 1% (w/w), pemberian pakan dengan penambahan ekstrak daun kayu manis 0,5% (w/w), kontrol positif, dan kontrol negatif dengan masing-masing perlakuan terdiri atas tiga ulangan. Hasil penelitian menunjukkan bahwa penambahan 1% (w/w) tepung daun kayu manis dalam pakan memberikan hasil terbaik dalam meningkatkan respons imun ikan patin dengan tingkat kelangsungan hidup sebesar 83,33% lebih tinggi dibandingkan kontrol positif (P<0,05).
Kata kunci: Aeromonas hydrophila, Cinnamomum burmannii, ekstrak, Pangasianodon hypophthalmus, tepung
Yeast stress and death caused by the synergistic effect of ethanol and SO<SUB>2</SUB> during the second fermentation of sparkling wines
Authors: Cisilotto Bruno , Scariot Fernando Joel , Vivian Schwarz Luisa , Mattos Rocha Ronaldo Kauê , Longaray Delamare Ana Paula , Echeverrigaray Sergio 
Published: October 2021

Problems can often arise at the beginning of the second fermentation (prise de mousse) of sparkling wines, such as no start, a long lag period or slow fermentation. These problems are generally associated with yeast stress when inoculated in a base wine with high ethanol content and low pH. However, few studies focus on sulphites, which are often added to base wines to prevent malolactic fermentation, microbiological instability, and wine oxidation. This study aimed to evaluate the joint effect of ethanol and sulfur dioxide on yeasts during the second fermentation. For this purpose, yeasts (Saccharomyces cerevisiae EC1118) were subjected to ethanol, sulfur dioxide and ethanol/sulfur dioxide at the beginning of fermentation, and their vitality and viability, as well as the accumulation of intracellular reactive oxygen species and intracellular pH, were evaluated by flow cytometry. Furthermore, the expression of genes involved in sulfur transport and metabolism was determined. The results showed high mortality, ROS accumulation and intracellular pH reduction in fermentations with both ethanol and sulfur dioxide. The negative effect of ethanol, sulfur dioxide and ethanol/sulfur dioxide on yeasts was found to be dose-dependent and high in those commonly found in some base wines. Cells treated with ethanol/sulfur dioxide showed over-expression of genes involved in sulphite transport (SUL1 and SUL2), efflux pump (SSU1 and FZF1) and metabolism of sulfur amino acids (MET14). Altogether, our data indicate that ethanol and sulfur dioxide have a synergistic effect on yeasts, which may be the root cause of the problems encountered at the beginning of the second fermentation of sparkling wines, and should thus be seriously taken into consideration by winemakers.
Dense Hyperthermic Intraperitoneal Chemotherapy with Cisplatin in Patients with Stage III Serous Epithelial Ovarian Cancer: A Retrospective Study
Authors: He Xiaoli , Wei Li , Li Rui , Jing Shuang , Jia Linlin , Ji Danwei , Li Yali , Wang Yue , Zhu Yongxia 
Published: October 2021

Abstract
Background: To investigate the efficacy and safety of interval debulking surgery (IDS) combined with dense hyperthermic intraperitoneal chemotherapy (HIPEC) with cisplatin in Chinese patients with FIGO stage III serous epithelial ovarian cancer (EOC).Methods: This retrospective single-center study reviewed the demographic and clinical data of 197 patients with primary FIGO stage III serous EOC who were treated with IDS with (n=121) or without (n=76, control group) dense HIPEC between January 2012 and April 2017. The co-primary endpoints were progression-free survival (PFS) and overall survival (OS), and the secondary endpoint was the occurrence of adverse events.Results: The median PFS was 24 months in the IDS plus dense HIPEC group, whereas it was 19 months in the IDS alone group (hazard ratio [HR] 0.46, 95% confidence interval [CI]: 0.33-0.65, p=0.000). The median OS in patients treated with IDS plus dense HIPEC (51 months) was significantly longer than that in patients treated with IDS alone (40 months, HR 0.52, 95% CI: 0.35-0.78, p=0.001). The demographic and preoperative clinical characteristics of these two groups were comparable (p>0.05). In the IDS alone group, no adverse events were recorded in 42 (55.3%) of the 76 patients, and 14 (18.4%) patients were reported to have grade III/IV adverse events. In the IDS plus dense HIPEC group, no adverse events were recorded in 55 (45.5%) of the 121 patients, and 23 (19.0%) patients were reported to have grade III/IV adverse events. No postoperative deaths occurred within 30 days in either group and neither did severe fatal complications in the IDS plus dense HIPEC group.Conclusions: IDS plus dense HIPEC with cisplatin in Chinese patients with FIGO stage III serous EOC is associated with improved survival and is reasonably well tolerated by patients.
Can Online Learning Fulfill the Competence of Medical Study Program Students?
Authors: Sundari Sri , Purbohadi Dwijoko , Khotibudin Muh 
Published: October 2021

Abstract
Background: The Covid-19 outbreak has had a huge impact on education, especially in health education both throughout the world and in Indonesia. Educational institutions are forced to close any access of offline meeting in order to reduce the spread of Covid-19. This is in accordance with the Official Letter of Ministry of Education and Culture Republic of Indonesia. No. 36962 / MPK.A / HK / 2020, the government prohibits universities from holding offline lectures then encourages online learning. Online learning in Indonesia is not popular before pandemic but it must be held. However, the learning goal must still be achieved. The learning process is aimed to achieve the expected performance also improve the quality of education. Quality assurance and personalized online learning are one of the mechanisms that help higher education to achieve planned outcomes. Online learning changes all aspects in the learning process. Both educators and students need to adapt to the online learning process. As a result, students and educators need to adapt quickly to minimize the material backlog. Due to the various reasons above, it is necessary to develop an online learning model that can help the learning process both in the cognitive and psychomotor domains in the Medical Study Program so that the expected competencies can still be achieved. Method: The data was analyzed by using t-test statistic to compare between students’ achievement in distance learning and offline learning.Results: The results showed that the learning outcomes of students who took online learning methods were higher than those of students who took offline learning. This can be seen from the average learning evaluation score, the number of passing practicum scores, the number of passing skills lab scores and the number of passing blocks (p<0.05). Conclusion: It can be concluded that online learning helps students achieve competence at the academic stage of learning.
Investigation of Biodesulfurization by A Novel Pseudomonas Strain and the Underlying Metabolic Mechanisms
Authors: Wei Dong , Liu Li , Shi Jiping , Yan Weizhi , Chen Xiaojia , Li Xiang 
Published: October 2021

Abstract
The increasing public expectations for good air quality have necessitated the development of biodeodorization technology. In this study, a novel heterotrophic Pseudomonas strain was isolated from biogas slurry and its biodesulfurization ability was investigated. The optimization of culture conditions determined 30 ℃ and 200 rpm as the optimal temperature and shaking speed. Under such conditions, 50 mM thiosulfate was completely consumed. The whole genome sequencing and functional annotation identified the genes associated with sulfur metabolism and the RT-qPCR analysis quantified the expression level of corresponding genes. The results showed that this strain was able to convert thiosulfate to sulfane, which could be decomposed into elemental sulfur and therefore achieved desulfurization. However, this process was relatively weak as most thiosulfate was converted into soluble tetrationate catalyzed by gene tsdA. Further research would focus on the enhancement of sulflane production pathway through metabolic engineering tools.
Effects of Vaccine Health and Safety Perceptions on COVID-19 Vaccine Uptake in Ghana: Implications for Implementing Rollout Programs
Authors: Aram Simon Appah , Hagan John Elvis , Mansoh George Kweku Afriyie , Saalidong Benjamin M. , Lartey Patrick Osei , Ahinkorah Bright Opoku , Seidu Abdul-Aziz , Ameyaw Edward Kwabena , Appiah Augustine , Hotor Divine Worlanyor , Gyimah Justice 
Published: October 2021

Abstract
Background A major component of rolled-out COVID-19 pandemic response and preparedness is the administration of vaccines. Globally, resistance towards vaccination programs are well known and documented. This study sought to evaluate the effects of general vaccine health perceptions and the confidence in COVID-19 vaccine safety towards uptake in Ghana. MethodsA cross sectional online survey involving 620 Ghanaians was conducted. The data was subjected to both descriptive (frequency, percentages, and chi-square tests) and inferential (nested binary logistic regression) analyses. Results The preliminary findings showed that 80.32% of participants believed that vaccines were healthy and 73.06% had confidence in a COVID-19 vaccine safety, although 81.19% of the respondents were particularly concerned about the source of the vaccine. Other evidence revealed that 78.55% and 71.45% of respondents indicated their willingness for mandatory and voluntary COVID-19 vaccine uptake or shot respectively. In all operationalized regression models, Ghanaians who believed that vaccines are healthy and those who had confidence in a COVID-19 vaccine safety were more likely to take a mandatory or voluntary COVID-19 vaccine compared to those who thought and believed otherwise. ConclusionIndividual preferences and/or intentions toward COVID-19 vaccine uptake and uptake route (i.e., mandatory, voluntary) were influenced by multifaceted determinants: biosocial (age, marital status, education), socio-cultural (religion) and contextual (geographical zone, source of vaccine as a concern) factors. To consolidate and possibly increase vaccine uptake in response to the COVID-19 pandemic in Ghana, health education and promotion programs should aim at creating awareness on the benefits of vaccine uptake while addressing the health and safety concerns on the potential side effects through evidence-based community messaging from credible sources. It is important to show speciﬁc commitment to transparency and reliable information to build public trust by decision-makers.
Nurses’ Perceptions of Continuing Professional Development: a Qualitative Study
Authors: Yu Xiaoyan , Huang Yi , Liu Yu 
Published: October 2021

Abstract
Background: Continuing professional development is regarded as one of the important approaches to maintaining skills and motivation for work. However, there is a lack of qualitative studies to explore Chinese nurses’ continuing professional development. The study aims to explore Chinese nurses’ perceptions of continuing professional development and challenges they face. Methods: The study was conducted in a tertiary hospital located in the central region of China from July to August 2020. Purposive sampling was used to recruit 14 nurses and face to face semi-structured interviews were conducted from July to August 2020. Then the recorded data were analysed and collated according to the thematic analysis. This study followed the consolidated criteria for reporting qualitative research (COREQ). Results: Four themes were extracted: improving specialty ability; different development phases; the importance of personal effort; the obstacle of work-family conflict.Conclusions: This study contributed to our understandings of nurses’ continuing professional development. Nurses held a positive attitude towards continuing professional development and they faced challenges in the meantime. Special attention and targeted supports should be provided to promote the continuing professional development of nursing staff.
Epigenetic Mechanism and Survival Prognosis Analysis of Serum Exosomes from Ovarian Cancer Patients based on Sequencing Technology and Bioinformatics
Authors: Xia Li , He Huang 
Published: October 2021

Abstract
Backguound: To screen the signaling axis of epigenetic modification in serum exosomes of ovarian cancer patients based on sequencing technology and raw signal analysis, in depth study of the potential mechanism of action of ovarian cancer, prediction of potential therapeutic targets and survival prognosis analysis of potential targets.Methods: Serum exosomes from three ovarian cancer patients were selected as the experimental group, and serum exosomes from three uterine fibroid patients as the control group, and whole transcriptome of serum exosomes was performed to obtain differentially expressed lncRNA and mRNA in ovarian cancer,The miRcode database and miRNA target gene prediction website were used to predict the target genes, Cytoscape software was used to draw a ceRNA network model of epigenetic modification of ovarian cancer serum exosomes, and the R language was used for GO and KEGG enrichment analysis of the target genes. Finally, the TCGA website was used to download clinical and expression data related to ovarian cancer, and the common potential target genes obtained in the previous period were analyzed for survival。Results: A total of 117 differentially expressed lncRNAs as well as 513 differentially expressed mRNAs (P < 0.05, |log2 FC|≥ 1.0) were obtained by combining sequencing data and raw signal analysis, and 841 predicted target genes were reciprocally mapped by combining mircode database and miRNA target gene prediction website, resulting in 11 potential target genes related to ovarian cancer (FGFR3, BMPR1B, TRIM29, FBN2, PAPPA, CCDC58, IGSF3, FBXO10, GPAM, HOXA10, LHFPL4), and survival prognosis analysis of the above 11 target genes revealed that the survival curve was statistically significant (P < 0.05) for HOXA10 only genes, but not for the other genes, and through enrichment analysis, we found that the above target genes were mainly involved in biological processes such as regulation of transmembrane receptor protein kinase activity, structural molecule activity with elasticity, transforming growth factor - activated receptor activity, and GABA receptor binding, and were mainly enriched in signaling pathways regulating stem cell pluripotency, bladder cancer, glycerolipid metabolism, central carbon metabolism of cancer, tyrosine stimulation to EGFR in signaling pathways such as resistance to enzyme inhibitors.Conclusions: The serum exosomal DIO3OS-hsa-miR-27a-3p-HOXA10 epigenetic modification signaling axis affects ovarian cancer development and disease survival prognosis by targeting transcriptional dysregulation pathways in cancer.
FUS Mutation Causes Lipid Metabolism Disorder in Skeletal Muscle Associated With ALS
Authors: Zhou Binbin , Li Xiaobing , Zheng Yilei , Dong Huifang , Yu Jiaxi , Zou Yang , Zhu Min , Yu Yanyan , Fang Xin , Zhou Meihong , Zhang Wei , Yuan Yun , Wang Zhaoxia , Deng Jianwen , Hong Daojun 
Published: October 2021

Abstract
Amyotrophic lateral sclerosis (ALS) is a devastating neurodegenerative disease, characterized by the dysfunction of upper and lower motor neurons accompanied by muscle weakness and wasting. Recently, several studies on ALS patients and animal models implicated muscle intrinsic toxicity played a role in the disease progression with uncertain mechanisms. In this study, we explored the possible disorder of lipid metabolism in myocytes associated with ALS. Initially, skeletal muscle from 41 ALS patients as well as 53 non-ALS control subjects were investigated, and identified that the accumulation of lipid droplets in the muscle fibers of ALS patients was significantly increased, especially in the patients with FUS mutations. A myoblasts (C2C12) cell model expressing FUS-K510Q mutant induced the accumulation of lipid droplets and mitochondrial dysfunction. Consistently, transgenic flies expressing FUS-K510Q under a muscle-specific driver showed elevated triglyceride levels in flight muscle, as well as locomotor defects. Biochemical analysis of C2C12 cells and fly muscle tissues showed up-regulation of PLIN2, and down-regulation of ATGL and CPT1A, indicating inhibition of lipolysis and fatty acid β-oxidation in muscle cells with FUS mutations. Our study provided a potential explanation for the lipid droplets accumulation in skeletal muscle of ALS. Our data also suggested that lipid metabolism disorder and mitochondrial dysfunction play a crucial role in the muscle intrinsic toxicity of ALS.
Unraveling MicroRNA Mediated Gene Regulation in Centella Asiatica (L.) Urb. by High-Throughput Sequencing based Small-RNA Profiling.
Authors: Ranjith Gouri Priya , Satheesan Jisha , Sabu K K 
Published: October 2021

Abstract
Centella asiatica is a widely spread herb mostly found in the tropics having extensive medicinal values. Here, we report for the first time, transcriptome-wide characterization of miRNA profile from the leaves of C. asiatica using high-throughput Illumina sequencing. We identified 227 conserved and 109 putative novel miRNAs. Computational screening revealed potential mRNA targets for both the conserved and novel miRNAs encoding diverse transcription factors and enzymes involved in plant development, disease resistance, metabolic and signaling pathways. Gene ontology annotation and KEGG analysis revealed the miRNA targets to be involved in a wide range of metabolomic and regulatory pathways. The differential expression of the miRNA encoding genes in diverse tissues was determined by real-time PCR analysis. We also found that gene expression levels of miR156, 159 and 1171 was reduced in salicylic acid treated axenic shoot cultures of C. asiatica compared to its control. Furthermore, RLM-RACE experiments mapped miRNA-mediated cleavage at two of the mRNA targets. The present study represents the large-scale identification of microRNAs from C. asiatica and contributes to the base for the up-coming studies on miRNA-mediated gene regulation of plant secondary metabolite pathways in particular.
Impact of the Gamma Variant on the ICU Admission in a Hospital of the Autonomous City of Buenos Aires (Caba), Argentina
Authors: Mandich Veronica , Chiacchiara Daniel , Bravo Marcelo , Pilipec Antonio , Grimaldi Santiago , Cotez Johnny , Paniouk Victor , Arevalo Henry , Escobar Oswaldo , Flach Sebastian Perez , Pozo Amilcar Herbas , Aimar Alejandro 
Published: October 2021

Abstract
Purpose

Comparison between patients admitted to the Intensive Care Unit (ICU) with ventilatory support during the first and second wave (Gamma variant) of COVID 19 in a tertiary hospital in the City of Buenos Aires.
Method

Descriptive, observational, prospective cohort study in ICU of an acute general hospital in Argentina, with patients adult patients COVID-19 admitted consecutively to the ICU. The ICU the main variables of interest: Demographic and laboratory data, history, duration of MV, mortality.
Results

44 patients were recruited during the first wave, and 80 in the second. Significant differences were observed in the second wave for age (60, [SD 11]; vs 68 [SD 11]; p <0.01), days with symptoms (9 [SD 5]; vs 4.5 [SD 3]; p <0.01), duration of MV (10 days [SD 7]; vs 16 [SD 13]; p <0.01), Ferritin (1450 mcg / l [SD 547]; vs 941 mcg / l [ SD 593]; p 0.04) and use of antibiotics (97% vs 45% p <0.01). There was no difference in mortality (66% vs 78% p 0.14) but there was in its primary causes; being more frequent hypoxemia associated with sepsis in the second wave (40% vs 3.4% p <0.01) and hypoxemia and multiorgan failure in the first (28% vs 4% p <0.01).
Conclusions

During the second wave of the pandemic, due to Gamma variant, more patients were admitted to our unit, younger, with a longer evolution time, a shorter time on MV and a greater inflammatory response, with no difference in mortality, although there was a difference in its causes.
Diode Pumped Alkali Laser - Current Status and Prospects
Authors: Endo Masamori , Nagaoka Hiroki , Wani Fumio 
Published: October 2021

Abstract
The historical aspect, present status, and future prospects of diode-pumped alkali lasers (DPAL) are discussed. DPAL is a relatively new laser, first reported in 2003. It is characterized by extremely high scalability, good beam quality by virtue of its gaseous laser medium, and intrinsically high efficiency because it is a three-level laser with a small Stokes defect. Because of these features, DPAL is being studied as a directed energy weapon and is also expected to find applications in industry and space. We propose the use of DPAL for space debris removal. Some of the research results of our group are also introduced.