bluuebunny/crossref_metadata_2025_split huggingface dataset:
Dissection of Two QTL&nbsp;for Grain Size Linked on the Long Arm of Chromosome 5 in Rice
Authors: Niu xiaojun , Zhang Zhenhua , Chan Aye Nyein , Fan Yeyang , Tang Shaoqing , Yu Sibin , Zhuang Jieyun , Zhu Yujun 
Published: April 2021

Abstract
Background: Most agronomical traits of crops are complex traits controlled by several major quantitative trait locus (QTL) and many minor QTL. Grain size determines grain weight and influences rice appearance quality. Identification of minor QTL is important for understanding the genetic and molecular network regulating grain size in rice. Following previous identification of QTL for grain weight and size using populations derived from the Teqing/IRBB52 indica rice cross, one QTL, qTGW5/qGL5 having significant effects on grain weight and length, was targeted for validation, dissection and fine-mapping.Result: Firstly, the effect of qTGW5/qGL5 was validated using two near isogenic line (NIL) F2 populations. Then, qTGW5/qGL5 was dissected into two closely linked QTL for grain size using four sets of NILs with sequential segregating regions. One of them, qTGW5 with the IRBB52 alleles increased grain weight, length and width with the same allelic direction, was located within an 1896.4-kb region flanked by RM18865 and Fi25273. The other one, qGL5 controlling grain length, was further delimited into a 68.8-kb region using seven NIL-F2 populations. Six annotated genes were found in the qGL5 region, of which five showed nucleotide polymorphisms between the two parental lines. In three of the six annotated genes, significant expression differences were detected between qGL5-NILs.Conclusions: Two closely-linked QTL having small effects for grain size in rice were separated using NIL-derived populations. One of them, qGL5 was fine-mapped into a 68.8-kb region containing six annotated genes. Our work lays a foundation for cloning minor QTL for grain size and offers potential targets for marker-assisted breeding in rice.
Bibliometric Analysis of the Top 100 Most Cited Articles on Wrist Arthroscopy
Authors: Gönder Nevzat , Kılınçoğlu Volkan 
Published: April 2021

Abstract
Background: Over the past few decades, more articles have been published about wrist arthroscopy. The purpose of this study was to identify and analyze the 100 most cited articles about wrist arthroscopy.Methods: The 100 most cited articles were analyzed using the wrist arthroscopy as a keyword on the Web of Science database. Original articles, reviews, clinical trials, and cadaveric studies were included in the study. We recorded and analyzed the following information: Article title, first author, year of publication, journal of publication, the total number of citations, level of evidence, article language, country, institution, and the main topic of study. Results: The number of citations ranged from 35 to 180 (mean, 64.74). The total number of citations was 6,474. Among the articles examined, the most prolific country was the United States of America (USA). The Journal of Hand Surgery- American Volume was the journal with the most number of publications and the most cited articles. The main topics that were most analyzed and underlined were the diagnostic comparison of Magnetic resonanas imaging and arthroscopy and the diagnosis and treatment of triangular fibrocartilage complex.Conclusions: As a result, wrist arthroscopy continues to develop, and it is a subject that is open for new studies. More comprehensive and up-to-date randomised controlled studies comparing the benefit of wrist arthroscopy or open surgery for wrist pathologies will demonstrate the importance of arthroscopy in the diagnosis and treatment of problems related to this topic.
Development of Chinese VAS Value Set for EQ-5D-3L and Difference analysis with the TTO Value Set Based on Same Nationally Representative Sample
Authors: Zhuo Lang , Zhuo Lin , Chen Siting , Cai Ting , Pan Jianqiang , Kong Yunxin , Jiang Haiting , Chen Jiaying 
Published: April 2021

Abstract
Objective: To develop a Chinese VAS value set for EQ-5D-3L and compare it with the TTO value set based on nationally representative sample. Methods: An adapted Measurement and Valuation of Health (MVH) protocol was applied with VAS method. EQ-5D-3L was used in face-to-face interviews conducted by trained interviewers with participants selected via multi-stage stratified clustered random sample. Fifteen hypothetical health statuses (11 random states in MVH protocol, plus 11111, 33333, unconscious and death) were assigned for assessment individually. Ordinary least square, general least square and weighted least square models were constructed with or without N3. Four categories of indices, including quality of original data, distribution of rescaled values, goodness of fit of models, and distribution of predicted values were adopted to compare between Chinese VAS and TTO value sets.Results: All 5,939 participants aged 15 and over were interviewed; 5,884 eligible participants were included in constructing models. An OLS model that included ten dummy variables and a constant and without N3 was chosen as the best-fit VAS model (Adjusted R2=0.670). The mean absolute error was 0.0319, and the correlation coefficient between predicted and mean values was 0.9837. The curve of predicted value of VAS model was uniformly lower than that of Chinese TTO value set. Comparing with the TTO value set, VAS method had higher responsive rate, less inconsistency, lower skewed values and better goodness of fit values. Conclusions: VAS value set performed rationally in Chinese population for its simplicity, responsiveness and robustness. We recommend the VAS value set, Model 2, as the benchmark of scoring algorithm of HRQoL for Chinese population.
Tumour suppressor gene methylation and renal cell carcinoma risk: a comprehensively systematic review and meta-analysis
Authors: Liu YuPeng , Ma Lin , Zhang YuXue , Wu ZhiGang , Liu Xiaodong 
Published: April 2021

Abstract
Purpose: Renal cell carcinoma (RCC) is becoming more common as a urinary system malignancy. There is a growing body of evidence supporting an important role of DNA methylation alteration involved in the initiation of RCC, but the current findings are inconsistent and controversial. Thus, we performed this systematic review and meta-analysis to comprehensively assess the associations between methylation status of tumour suppressor genes and the incidence risk of RCC. Methods: This study has been registered on PROSPERO (CRD42019130782) and was reported according to the PRISMA guidelines. We systematically searched the PubMed, EMBASE and CNKI databases for relevant studies. The effect estimates were summarized using random-effect models. Results: A total of 21 case-control studies containing 1,912 participants were included. Overall, abnormal hypermethylation of RASSF1A was associated with a significantly increased risk of RCC (OR, 6.612, 95% CI: 1.926-22.697, P = 0.003), especially in the American populations (OR, 18.429, 95% CI: 3.072-110.536, P = 0.001). An increased RCC risk was also associated with hypermethylation of SFRP1 and GSTP1 (OR, 3.995, 95% CI: 1.607-9.934, P = 0.003; OR, 4.508, 95% CI: 1.004-20.239, P = 0.049; respectively); however, the results for SFRP1 and GSTP1 were non-conclusive due to the limited number of studies included and the inconsistency across sensitivity analyses. There was no obvious association for the other genes. Conclusion: This study demonstrated a statistically and robustly positive association of aberrant hypermethylation of RASSF1A with an increased risk of developing RCC, indicating a potentially useful biomarker to predict the RCC incidence risk.
Is the Health-Awareness of Leaders Related to the Working Conditions, Engagement, and Exhaustion in Their Teams? A Multi-Level Mediation Study.
Authors: Grimm Luisa A. , Bauer Georg F. , Jenny Gregor J. 
Published: April 2021

Abstract
Background: Research shows that leaders influence the health and well-being of employees, by being either a buffer or major source of employee's work stressors. Various leadership behaviors and their relation to employee outcomes have been examined. Yet, a satisfactory explanation of how leaders’ behavior influences health has not been found. A new line of research investigates the construct of “health-oriented leadership”, that is, the health awareness of leaders towards themselves (“self-care”) and towards their employees (“staff-care”). It is hypothesized that this health-orientation has a direct effect on both leader’s and employees’ health, as well as an indirect effect mediated by their working conditions.Methods: Data were derived from four company research projects, that involved employee and leader surveys on work, health, and well-being. The sample consisted of 50 teams, with 191 leaders and 604 team members. To test the relation between a leader’s self-care and his/her engagement, exhaustion, as well as staff-care, multiple regression analyses and mediation analyses were conducted. The relation between a leader’s staff-care, the team’s job resources and demands, and the individual employee outcomes engagement and exhaustion were tested with multilevel analyses.Results: Regression analysis showed that the stronger a leader's health-orientation towards him/-herself (“self-care”), the stronger was the health-orientation towards his/her employees (“staff-care”). A leader’s self-care was also associated with higher work engagement and lower exhaustion and this relation was mediated by his/her job resources and demands, respectively. Multilevel analysis showed that a leader's staff-care was associated with employee work engagement and exhaustion, and that this relation was mediated by team-level job resources and demands, respectively.Conclusions: The health-orientation of leaders relates to their own as well as their teams' engagement and exhaustion, which is partly mediated by job demands and resources. Thus the construct of health-orientation may prove worthy of further exploration. For practical conclusions, this study provides support for researching not different leadership styles with very specific facets, but a general orientation towards health, which can be implemented into coaching and consulting sessions for organizations.
Obstructed descending colon mass pretended with Bockdalek hernia: a case report
Authors: eslamian mohammad , kolahdouzan Mohsen 
Published: April 2021

Abstract
Introduction: Adult Bochdalek hernia is one of the right-sided diaphragmatic hernias that less than 30 cases reported until now.Case: I herein report a 64-year-old female patient who had dyspnea, abdominal pain, and nausea. Primary imaging (thoracic and abdominal CT scan) showed a right-sided diaphragmatic hernia that was contained the liver and right colon. The patient underwent right posterior thoracotomy at first, so the 5*5 cm diaphragmatic defect was repaired. Due to peritonitis that happened after two days, a midline laparotomy was performed. Finally, it was cleared that the main problem was the obstructed and perforated descending colon mass that was presented with Bockdalek hernia. Unfortunately, she died.Conclusion It is important to determine the reason for the presentation of the symptomatic diaphragmatic hernia in adult patients. It should be considered that an increase of intra-abdominal pressure like the presence of obstructed colon mass can cause it.
Economic burden and prevalence of malaria in companies in Douala, Cameroon
Authors: Nchetnkou Christian Mbohou , Nyabeyeuh Herve Nyabeyeuh , Tonga Calvin , Kangam Lafortune , Foko Loick Pradel Kojom , Nono Larissa Kouodjip , Yamadji Linda Arlette Ngapmen , Kemajou Gaelle Mbiakop , Bayoi Charlie Ngo , Mbongo Thyerry Adolphe Dikoume , Noubou Sorelle Wakam , Mbou Isabelle Matip , OTTOU Blaise , Nolla Nicolas , Priso Dina Nfon , Ndema Péguy Assomo , Lehman Leopold Gustave 
Published: April 2021

Abstract
Background: Malaria is a public health concern in Africa. At the socio-economic level, it represents a brake on the national growth through an increase of health spending, drop of productivity and high rate of absenteeism at the workplace. Some companies have implemented malaria control measures to improve their performance, but the vast majority are slow to follow this approach. Using the cost-of-illness approach, the objective of this study was to estimate the economic impact of malaria in two companies with 24-hour daily activity based in Douala, Cameroon: an agro-food industry nicknamed "AGRO" and a security company nicknamed "SECU".Method: A retrospective analysis of medical and financial records of these companies was carried out to calculate the economic cost of absenteeism, prophylaxis, Internal Management of Malaria cases (IMM) and External Management of Malaria cases (EMM) to these companies. A malaria screening campaign was carried out also in each company in one day from 8 a.m. to 4 p.m. The clinical, anthropometric and socio-demographic data of the employees were collected before the collection of a drop of blood which was subsequently analyzed under the Cyscope fluorescence microscope (Partec-Sysmex, Münster, RFA). Results: Analysis of retrospective data revealed that the prevalence of symptomatic malaria cases had decreased by 8.9% and 25.1% respectively at AGRO and SECU between 2010 and 2012. Regarding the prospective component, 372 employees of the two companies were included in the study. The prevalence of malaria was 26.83% and 26.44% respectively at AGRO in January 2013 and SECU in February 2013. Malaria-related absenteeism accounted for 5% and 17% of general absenteeism at AGRO and SECU respectively. The cost of an EMM accounted for 19.5% and 40.5% of the monthly income of AGRO and SECU workers respectively. Each year, malaria generates a shortfall of US$ 26,426 and US$ 136,824 respectively to AGRO and SECU. Conclusion: Malaria have a negative impact on companies. However, early detection of suspected cases, campaigns to distribute insecticide-treated mosquito nets, Information, Education and Communication within the company could reduce this impact.
Preparation of ordered Ag@Au hybrid nanotubes arrays and their fluorescence enhancement of poly(3-hexylthiophene)
Authors: Jianchao Wang , Tang Gege , Peng Huayong 
Published: April 2021

Abstract
The ordered silver nanowires (Ag NWs) were assembled by three-phase interface method and the ordered Ag@Au hybrid nanotubes were successfully prepared by the galvanic replacement reaction between HAuCl4 solution and the ordered Ag NWs. Field emission scanning electron microscope (FESEM) and transmission electron microscope (TEM) were employed to analyze the morphology of the ordered Ag NWs and Ag@Au nanotubes, and the influence of reaction time in displacement reaction. Fluorescence and laser-Raman properties of conjugated polymerpoly(3-hexylthiophene) (P3HT) were analyzed on various ordered Ag@Au nanotubes on the fluorescence. The results showed that the Au particle grew on ordered Ag@Au hybrid nanotubes with the reaction time going. The fluorescence properties of P3HT films are improved on various ordered Ag@Au hybrid nanotubes compared with those on bare silicon substrate, but the fluorescence intensity of P3HT films on the ordered hybrid nanowires decreases as the galvanic displacement process.
Decreased Expression of CBX7 is an Independent Predictor of Poor Survival in Cervical Cancer
Authors: An Lanfen , Zhang Jun , Jin Zhishan , Yan Xia , Wang Pu , Ouyang Weixiang , Wang Hongbo 
Published: April 2021

Abstract
Background: CBX7, a component of the PRC1, has been investigated as a potential biomarker in human malignant neoplasias. In present study, the value of CBX7 expression in the diagnostic and prognosis of cervical squamous cell carcinoma and endocervical adenocarcinoma (CESC) as examined via bioinformatics analysis of data obtained from Genotype-Tissue Expression (GTEx) database and The Cancer Genome Atlas (TCGA) database.Methods: Relationships between clinical factors and CBX7 were explored. The Kaplan-Meier method and Cox regression were used to identify the associations between clinicopathological characteristics and overall survival (OS) in CESC. Gene set enrichment analysis (GSEA) was performed using TCGA dataset. Results: Our results indicated the decreased expression of CBX7 in CESC, and difference in CBX7 expression was also identified according to age subgroups. CESC patients with decreased CBX7 expression had worse prognosis than those with high CBX7 expression. Multivariate analysis showed that CBX7 was an independent risk factor for OS. GSEA demonstrated pathways involved in the biosynthesis of unsaturated fatty acids, glycosaminoglycan biosynthesis-chondroitin sulfate, glyoxylate and dicarboxylate metabolism, nod-like receptor signaling pathway, O-glycan biosynthesis, one carbon pool by folate and protein export as differentially enriched in CESC with decreased CBX7 expression.Conclusion: We demonstrated that decreased CBX7 expression may be a potential independent biomarker for poor prognosis in CESC.
The complete plastomes of red fleshed pitaya (Selenicereus monacanthus) and three related Selenicereus species: insights into gene losses, IR expansions and phylogenomic implications
Authors: Qin Qiulin , Li Jingling , Zeng Siyuan , Xu Yiceng , Han Fang , Yu Jie 
Published: April 2021

Abstract
Background: Selenicereus is a genus of perennial shrub from the family Cactaceae, and some of them play an important role in the food industry, pharmaceuticals, cosmetics and medicine. To date, there are few reports on Selenicereus plastomes, which limits our understanding of this genus. Here, we reported the complete plastomes of four Selenicereus species (S. monacanthus, S. annthonyanus, S. grandiflorus and S. validus, and carried out a comprehensive comparative analysis.Results: The four Selenicereus plastomes all have a typical quartile structure. The plastome size ranged from 133,146 bp to 134,450 bp, and contained 104 unique genes, including 30 tRNA genes, 4 rRNA genes and 70 protein-coding genes. Comparative analysis showed that there were massive losses of ndh genes in Selenicereus. Besides, we observed the IR regions had undergone a dramatic expansion and formed a previously unreported SC/IR border in the intron region of the atpF gene. Furthermore, we identified 6 hypervariable regions (trnF-GAA-rbcL, ycf1, accD, clpP-trnS-GCU, clpP-trnT-CGU and rpl22-rps19) that could be used as potential DNA barcodes for the identification of Selenicereus species. Phylogenetic analysis indicated that Hylocereus was nested in Selenicereus.Conclusion: Our study enriches the plastomic resources in the family Cactaceae, and provides the basis for the reconstruction of phylogenetic relationships.
Trace Analysis of Emerging Virus: an Ultrasensitive ECL-Scan Imaging System for Viral Infectious Disease
Authors: Wu Yunxia , Wu Qikang , Fu Yu , Huang Mingxing , Fan Zhijin , Shu Bowen , Wang Jingru , Wu Wenjie , Chen Xiaodong , Yang Ronghua , Xia Jinyu , Liao Yuhui 
Published: April 2021

Abstract
BackgroundEmerging infectious disease have brought a huge impact on human society in recent years. The outbreak of Zika virus (ZIKV) in the Americas resulted in a large number of babies born with microcephaly. More seriously, the Coronavirus Disease 2019 (COVID-19) caused the global spreads and immeasurable damages. Thus, the monitoring of highly pathogenic virus is of significance to the prevention and control of emerging infectious disease.ResultsHerein, a dendritic polymer probe-amplified ECL-scan imaging system was constructed to realize trace analysis of viral emerging infectious disease. Dendritic polymer probe was employed as the efficient signal giving-out component that could generate amplified electrochemiluminescence (ECL) signal on the integrated chip. And the signal was detected by a single-photon level charge coupled device-based ECL-scan imaging system. With this strategy,the ZIKV in the complex system of blood, urine and saliva were detected. The results indicated that high sensitivity of 50 copies and superior specificity were achieved. Furthermore, this strategy realized highly sensitive detection (10 copies) of S and N protein gene sequence of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-Cov2) and spiked pseudovirus samples.ConclusionsThus, the dendritic polymer probe-amplified ECL-scan imaging system suitably met the strict clinical-requirements for trace analysis of emerging virus, and thus has the potential to serve as a paradigm for monitoring of emerging infectious disease.
Z-scheme CdS/BiVO4&nbsp;Photocatalyst for Highly Efficient Photodegradation of Organic Pollutants and DFT Calculation
Authors: Shan Lianwei , Yuan Mingqi , Ma Chengguo , Lu Changhui , Fang Zilan , Lin Shuituan , Li Dan , Wu Ze , Dong Limin , Qian Guolin , Yang Lixin 
Published: April 2021

Abstract
In the field of photocatalysis, the heterojunction strategy is one of the effective ways to settle the problems of rapid recombination and sluggish mobility of the photo-generated carriers. Herein, we used a strategy of electrophoretic deposition to synthesize Z-scheme CdS/BiVO 4 . Interestingly, compared with the pristine CdS and BiVO 4 , the prepared CdS/BiVO 4 showed effective charge separation, high degradation efficiency and well photocatalytic stability. It demonstrated that electrophoretic deposition can successfully construct photocatalyst steadily and effectively heterostructured CdS/BiVO 4 . Meanwhile, the electron transfer paths between BiVO 4 and CdS were probed by the DFT calculations. We infer that there is a Z-scheme mechanism between BiVO 4 and CdS. Moreover, this efficient strategy can support more applications for more efficient composition design of semiconductor heterojunctions.
Synthesis, Characterization and Biological Studies on 3d-Metal Complexes of 5-[(1, 5-Dimethyl-3-oxo-2-Phenyl-2, 3-Dihydro-1H-Pyrazol-4-yl) Diazenyl]-1-Ethyl-6-Hydroxy-4-Methyl-2-oxo-1, 2-Dihydropyridine-3-Carbonitrile
Authors: G Krishnamurthy , Melkeri Shridhar P , Naik P. Parameshwara , Walmik Prabhaker , Priyarani R S 
Published: April 2021

Abstract
N-hetorocyclic ligand. 5-[(1,5-dimethyl-3-oxo-2-phenyl-2,3-dihydro-1H-pyrazol-4-yl)diazenyl]-1-ethyl-6-hydroxy-4-methyl-2-oxo-1,2-dihydropyridine-3-carbonitrile (L) and a few of their transition metal complexes have been synthesised and characterized by different physico-chemical techniques. The electronic and magnetic susceptibility measurements suggest an octahedral geometry for all the complexes excepting Zn(II) complex which possess tetrahedral geometry. The results of thermogravimetric analysis showed that the synthesized complexes have thermal stability and confirms the absence of coordinated or lattice water molecules. The bio potency of all the compounds were investigated by studying the antimicrobial, antimycobacterial and cytotoxic activities. All the transition metal complexes were noticeably exhibited their inhibition properties against the tested microbes.