bluuebunny/crossref_metadata_2025_split huggingface dataset:
Construction and characterization of bioluminescent
Salmonella
Reading outbreak and non-outbreak strains
Authors: Isah Abubakar Shitu , Ramachandran Reshma , Sukumaran Anuraj Theradiyil , Kiess Aaron S. , Castañeda Claudia D. , Boltz Timothy , Macklin Kenneth S. , Abdelhamed Hossam , Zhang Li 
Published: December 2024

ABSTRACT

Salmonella
enterica serotype Reading has recently been identified as a significant foodborne pathogen from contaminated poultry products. There is a critical need for close monitoring of this newly emerged pathogen. This study developed bioluminescent strains of
S.
Reading for real-time pathogen tracking using bioluminescence imaging. Two strains of
S
. Reading were used: an outbreak strain and a non-outbreak strain. The chloramphenicol acetyltransferase gene was cloned into the plasmid pBS-
slp
GFP
luxABCDE
, which carries
luxABCDE
operon and an ampicillin resistance gene. The newly constructed plasmid was then transformed into the outbreak and non-outbreak strains of
S
. Reading by electroporation. The resulting colonies were confirmed by visualizing bioluminescence using an
in vivo
imaging system and by testing their resistance to chloramphenicol. These strains demonstrated a high bioluminescence level (10
8
–10
9
Photons/s/cm
2
/sr) and were tested for growth and plasmid stability by daily subculturing in Luria–Bertani medium with and without antibiotics. The plasmid remained stable for 8 days under non-selective conditions, and growth rates were comparable to non-bioluminescent parent strains in antibiotic-free conditions. However, growth was notably different in the presence of chloramphenicol, indicating successful plasmid retention and function. This study successfully created stable bioluminescent
S
. Reading strains, marking a significant step forward in monitoring and potentially reducing the spread of this emergent foodborne pathogen in the poultry industry.

IMPORTANCE

Salmonella enterica
serotype Reading has recently become a significant foodborne pathogen linked to poultry products. To enhance pathogen monitoring, this study developed bioluminescent strains of
S.
Reading by inserting the chloramphenicol acetyltransferase gene into a plasmid containing a bioluminescence gene cluster. These modified strains were transformed into outbreak and non-outbreak bacterial strains via electroporation. The bioluminescent strains demonstrated stable plasmid retention and high bioluminescence levels. They also showed growth comparable to their parent strains, even in the absence of antibiotics. These bioluminescent strains could potentially facilitate real-time monitoring and control of
S.
Reading in poultry industries.
Efficacy of carbonyl cyanide-3-chlorophenylhydrazone in combination with antibiotics against
Mycobacterium abscessus
Authors: Zheng Jifang , Liu Qiang , Wang Haoran , Huo Fengmin , Jia Junnan , Huang Hairong , Chen Suting 
Published: December 2024

ABSTRACT

Given the intrinsic resistance of
Mycobacterium abscessus
to a wide range of conventional antibiotics, it is urgent to explore new therapeutic approaches to manage this infection effectively. Carbonyl cyanide 3-chlorophenylhydrazone (CCCP), a proton pump inhibitor, has shown good bacteriostatic activity against
M. abscessus
. This study aimed to determine its synergistic antimicrobial effects when combined with commonly used antibiotics. The
M. abscessus
reference strain and 39 clinical isolates were collected. The minimum inhibitory concentration and fractional inhibitory concentration index were determined for the combined treatment using CCCP with various antibiotics including clarithromycin, amikacin, linezolid, bedaquiline, and clofazimine. A time-killing assay was used to measure the effect of the combined drug regimens quantitatively. The simultaneous use of CCCP with traditional antibiotics shows a synergistic effect across a wide range, significantly boosting the ability to inhibit the growth of
M. abscessus
throughout its growth phases. When CCCP is used in combination with clarithromycin, amikacin, and linezolid, it produces a synergistic effect on both the standard strain and most clinical isolates. When CCCP is paired with bedaquiline and clofazimine, it exhibits additive effects. Moreover, high levels of CCCP in combination with other antibiotics were found to rapidly eradicate the bacteria. The use of CCCP as a potential treatment for
M. abscessus
infections shows promising results, especially when combined with other antibiotics to achieve a potent bactericidal effect.

IMPORTANCE

Mycobacterium abscessus
poses a significant public health threat due to its intrinsic resistance to a broad spectrum of conventional antibiotics. This resistance necessitates urgently exploring novel therapeutic strategies to effectively combat infections caused by this pathogen. Our previous research has identified carbonyl cyanide-3-chlorophenylhydrazone (CCCP) as a potent direct antimicrobial agent against
M. abscessus
and as an enhancer of clarithromycin activity. Our results demonstrate that the concurrent administration of CCCP with traditional antibiotics exhibits a synergistic effect across a wide range, which could be crucial for overcoming the challenges posed by
M. abscessus
infections. Furthermore, the use of high concentrations of CCCP in combination with other antibiotics was found to rapidly eliminate
M. abscessus
, suggesting a potential therapeutic advantage. These insights not only advance our understanding of antimicrobial synergy but also hold promise for the development of more effective treatment regimens against drug-resistant
M. abscessus
infections.
Trends in the survival of very preterm infants between 2011 and 2020 in France
Authors: Butler Victoria , Gaulard Luc , Sartorius Victor , Ancel Pierre Yves , Goffinet François , Fresson Jeanne , Zeitlin Jennifer , Torchin Héloïse 
Published: December 2024

ObjectiveThe objective is to evaluate changes in survival to discharge of liveborn infants less than 32 weeks’ gestational age (GA) in France, where the latest available data on very preterm survival at a national-level are from the EPIPAGE-2(Etude épidémiologique sur les petits âges gestationnels)cohort in 2011.DesignPopulation-based cohort study.SettingMetropolitan France in 2011, 2015 and 2020.PatientsAll births between 22 and 31 weeks’ GA using the EPIPAGE-2 cohort study for the year 2011 and hospital discharge data linked to death certificates from theSystème National des Données de Santéfor the years 2015 and 2020.Main outcome measuresThe primary outcome was survival to hospital discharge among liveborn infants. Survival rates were compared using modified Poisson regression and adjusted for population characteristics (maternal age, multiple birth, sex, small for GA). Data on all births were examined to assess changes to the live birth rate.ResultsSurvival to discharge among live births increased at 23 and 24 weeks’ GA from 1% and 31% in 2011 to 8% and 37% in 2015 and to 31% and 47% in 2020, respectively. From 25 to 28 weeks’ GA, survival rates tended to increase, but differences were not significant, and survival rates were stable from 29 to 31 weeks GA. Results were similar after adjustment. The proportion of live births versus stillbirths increased from 22 to 24 weeks’ GA.ConclusionSurvival rates among live births improved between 2011 and 2020 from 23 to 28 weeks’ GA, with marked changes at 23 and 24 weeks’ GA.
Long COVID among the first three waves of COVID-19 in Japan: a multicentre cohort study
Authors: Takaoka Hatsuyo , Kawada Ichiro , Hiruma Gaku , Nagashima Kengo , Terai Hideki , Ishida Noriyuki , Namkoong Ho , Asakura Takanori , Masaki Katsunori , Miyata Jun , Chubachi Shotaro , Ohgino Keiko , Otsuka Kengo , Miyao Naoki , Odani Toshio , Watase Mayuko , Baba Rie , Okamori Satoshi , Arai Daisuke , Nakachi Ichiro , Kuwahara Naota , Fujiwara Akiko , Okada Takenori , Ishiguro Takashi , Isono Taisuke , Ishii Makoto , Sato Yasunori , Fukunaga Koichi 
Published: December 2024

ObjectivesSevere acute respiratory syndrome coronavirus 2 significantly impacts Japan with a high number of infections and deaths reported. Long coronavirus disease (COVID) characterised by persistent symptoms after COVID-19 has gained recognition but varies across studies. This study aimed to investigate the differences in long COVID among patients hospitalised during Japan’s first three waves of the pandemic.DesignMulticentre prospective cohort study.Setting26 medical facilities across Japan between February 2020 and February 2021.ParticipantsIn total, 1066 hospitalised patients diagnosed with COVID-19 were included with 206, 301 and 559 patients in the first, second and third waves, respectively. Data were collected using electronic data capture and patient-reported outcome forms.Primary and secondary outcome measuresLong COVID was assessed at 3, 6 and 12 months after COVID-19 diagnosis.ResultsSignificant differences were observed between the waves in various baseline and clinical characteristics such as age, body mass index (BMI), comorbidities, the severity of COVID-19, complications and treatment during hospitalisation. Long COVID, particularly dyspnoea, was most prevalent in the first wave. Multivariate logistic regression analysis confirmed a significant positive association between the first wave and long COVID including dyspnoea after adjusting for age, sex, BMI, smoking status and COVID-19 severity.ConclusionsPatients hospitalised during the first wave had a higher risk of experiencing long COVID, especially dyspnoea, than those hospitalised during the other waves. These findings underscore the need for continued monitoring and managing long COVID in COVID-19 survivors, particularly in those hospitalised during the first wave.Trial registration numberUMIN000042299.
How do patients determine when their inhaler is empty? Insights from an analysis of returned inhalers and a patient survey
Authors: Murphy Anna C , Carroll Will , Gotsell Marissa , Potter Charles , Quint Jennifer K , Malone Rachel 
Published: December 2024

BackgroundInhalers are widely used for the management of asthma and chronic obstructive pulmonary disease. However, there is little knowledge about the extent to which an inhaler is used and when it is disposed of, despite the implications for an individual’s health (when used beyond the recommended number of doses (overused)), and medicine wastage, healthcare costs and the environment (when discarded with remaining doses (underused)). To explore inhaler use, we assessed the number of doses remaining in pressurised metered-dose inhalers (pMDIs) returned via a Chiesi Inhaler Recycling scheme.MethodspMDIs were dismantled, and components recycled where possible. Each canister was weighed and the mass of the formulation remaining was calculated. pMDIs were categorised based on number of doses remaining (underused, used, empty (indicating correct use) and overused) and by dose counter presence/absence. A separate online survey was used to obtain patient feedback on inhaler use and disposal behaviours.ResultsOverall, 2614 pMDIs were analysed (55.9% maintenance, 44.1% reliever inhalers); 1015 (38.8%) had an integrated dose counter. The proportion of pMDIs returned empty was greater for inhalers with dose counters than for those without (51.3% vs 25.1%; p<0.0001); the proportion of pMDIs returned underused was lower for inhalers with dose counters than for those without (5.2% vs 33.2%; p<0.0001). The proportion of pMDIs returned overused was substantial and similar for devices with and without dose counters (34.0% vs 23.2%; p>0.01). Most respondents (55.2%) using devices without a dose counter reported that they were not confident in identifying when their inhaler was empty. Furthermore, many respondents (20.6%) who used inhalers with a dose counter reported continued use beyond ‘zero’.ConclusionsOur study suggests that many inhalers are returned underused or overused, with inadequate knowledge among patients about the number of therapeutic doses remaining in the device and appropriate inhaler disposal. These have concerning implications for patient health and the environment and highlight a need for high-quality education for patients and healthcare professionals.
Predictive models of injury risk in male professional football players: a systematic review
Authors: Martins Francisco , Przednowek Krzysztof , Santos Francisco , França Cíntia , Martinho Diogo , Gouveia Élvio Rúbio , Marques Adilson , Sarmento Hugo 
Published: December 2024

BackgroundOne of the challenges for professional football players is injuries. Due to their influence on their teams, injuries greatly impact the sports business. This research aims to assess predictive models of injury risk in male professional football players.MethodsA systematic literature review was performed, following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines. The search was conducted in the PubMed, Web of Science and Scopus databases. Two independent reviewers screened articles, assessed eligibility and extracted data. Methodological quality was determined by the Newcastle–Ottawa Scale.Results26 studies met the inclusion criteria.DiscussionVarious statistical techniques were used in research on injury prediction in professional football, with logistic regression being the most used. The assessment predictors, especially the area under the receiver operating characteristic Curve, showed significant variation, which indicates the prediction models’ efficacy. The focus was frequently on lower limb injuries, where several risk predictors, including muscular strength, flexibility and global positioning system-derived data, were found to substantially impact the occurrence of injuries. Prominent predictors included age, position, physiological parameters, injury history and genetic polymorphisms.ConclusionsThis comprehensive analysis highlights the complexity of injury prediction and reinforces the necessity for football injury research to adopt a multivariate approach with accuracy and comprehensiveness.PROSPERO registration numberCRD42023465524.
Non-fatal drug overdose surveillance using hospital discharge data: a comparison between the Drug Overdose Surveillance and Epidemiology (DOSE) system and the Healthcare Cost and Utilisation Project, 18 states, 2018–2020
Authors: Quader Zerleen S , Park Joohyun , Krishnan Snehaa D , Stokes Erin , Pickens Cassandra M 
Published: December 2024

BackgroundThe Centers for Disease Control and Prevention’s Drug Overdose Surveillance and Epidemiology (DOSE) system captures non-fatal overdose data from health departments’ emergency department (ED) and inpatient hospitalisation discharge data; however, these data have not been compared with other established state-level surveillance systems, which may lag by several years depending on the state. This analysis compared non-fatal overdose rates from DOSE discharge data with rates from the Healthcare Cost and Utilization Project (HCUP) in order to compare DOSE data against an established dataset.MethodsDOSE discharge data case definitions (ie, International Classification of Diseases, 10th revision, Clinical Modification codes) for non-fatal unintentional/undetermined intent all drug, all opioid-involved, heroin-involved and stimulant-involved overdoses were applied to HCUP’s 2018−2020 State Emergency Department Databases (SEDD) and State Inpatient Databases (SID). Quarterly crude rates (per 100 000 population) and rate differences of four overdose categories were calculated for ED and inpatient data sources across 18 states included in DOSE and HCUP datasets for at least 2 consecutive years. Joinpoint regression models examined trends from 2018 through 2020, estimating average quarterly percentage change (AQPC) and 95% CIs.ResultsQuarterly crude rate differences between DOSE ED and SEDD data (across 12 states) and DOSE inpatient and SID data (across 16 states) indicated that 82% and 93% of rates, respectively, were within ±0.5 non-fatal overdoses per 100 000 population of each other. AQPC across states and drug categories were similar between the two data sources for both ED and inpatient data.DiscussionNon-fatal overdose surveillance through DOSE discharge data may be a valid and timely source for estimating non-fatal overdoses at the state level.
Search for baryon junctions in e+A collisions at the electron ion collider
Authors: Magdy Niseem , Deshpande Abhay , Lacey Roy , Li Wenliang , Tribedy Prithwish , Xu Zhangbu 
Published: December 2024

Abstract
Constituent quarks in a nucleon are the essential elements in the standard “quark model” associated with the electric charge, spin, mass, and baryon number of a nucleon. Quantum chromodynamics (QCD) describes nucleon as a composite object containing current quarks (valence quarks and sea (anti-)quarks) and gluons. These subatomic elements and their interactions are known to contribute in complex ways to the overall nucleon spin and mass. In the early development of QCD theory in the 1970s, an alternative hypothesis postulated that the baryon number might manifest itself through a non-perturbative configuration of gluon fields forming a Y-shaped topology known as the gluon junction. In this work, we propose to test such hypothesis by measuring (i) the Regge intercept of the net-baryon distributions for e+(p)Au collisions, (ii) baryon and charge transport in the isobaric ratio between e+Ru and e+Zr collisions, and (iii) target flavor dependence of proton and antiproton yields at large rapidity, transported from the hydrogen and deuterium targets in

$$e+p$$

e
+
p

(d) collisions. Our study indicates that these measurements at the EIC can help determine what carries the baryon number.
The Hemodynamic Management and Postoperative Outcomes After Cytoreductive Surgery and Hyperthermic Intraperitoneal Chemotherapy: A Prospective Observational Study
Authors: Solanki Sohan Lal , Agarwal Vandana , Ambulkar Reshma P. , Joshi Malini P. , Chawathey Shreyas , Rudrappa Shivacharan Patel , Bhandare Manish , Saklani Avanish P. 
Published: December 2024

Background: Cytoreductive surgery with hyperthermic intraperitoneal chemotherapy (CRS‐HIPEC) has become standard treatment for peritoneal cancers and metastases, significantly enhancing survival rates. This study evaluated the relationship between tumor burden, hemodynamic management, and postoperative outcomes after CRS‐HIPEC.Methodology: This study included 203 patients undergoing CRS‐HIPEC. The study was registered with ClinicalTrials.gov (NCT02754115). Routine and advanced hemodynamic monitoring was performed. Data on fluid and blood transfusions, coagulation management, body temperature, blood gases, Peritoneal Carcinomatosis Index (PCI), and chemotherapeutic agents used were collected. Postoperatively, complications using the Clavien–Dindo classification were employed. Primary outcomes assessed PCI’s impact on hemodynamic parameters and fluid management, with secondary outcomes including postoperative complications, mortality, and length of ICU and hospital stays.Results: Patients with PCI > 20 experienced significantly longer surgeries (796.2 ± 158.3 min) as compared with patients with PCI 0–10 (551 ± 127 min) and patients with PCI between 11 and 20 (661.78 ± 137.7 min) (p ≤ 0.01). Patients with PCI > 20 received higher fluid requirements (mean: 5497.7 ± 2401.9 mL) as compared with PCI 0–10 (2631.2 ± 1459.9 mL) and PCI 10–20 (3964.65 ± 2044.6 mL) (p ≤ 0.01). Patients with PCI > 20 also had a prolonged ICU stays (median: 4 days) as compared with PCI 0–20 (median: 3 days). However, these differences were not significant in patients with PCI between 10 and 20. Significant differences in CI and SVI were observed among PCI groups during and after HIPEC. Significant differences were also observed among PCI groups for postoperative complications. Although 30‐day survival rates varied clinically, they did not reach statistical significance.Conclusion: A higher PCI score was significantly associated with increased duration of surgery, fluid requirements, the need for invasive hemodynamic monitoring, postoperative complications, and longer ICU stays. Tailoring perioperative strategies based on PCI scores has the potential to optimize these outcomes.Trial Registration: ClinicalTrials.gov identifier: NCT02754115.
Assessing Critical Care Nurse’s Knowledge and Adherence to Evidence‐Base Guidelines for Ventilator‐Associated Pneumonia Prevention in Palestinian Hospitals
Authors: Ahmad Al-Nawaja’a Ismael , Salameh Basma , Toqan Dalia , Hammad Bahaaeddin M. , Fashafsheh Imad 
Published: December 2024

Background: Patients in critical care units who are connected to mechanical ventilators (MV) often face the risk of ventilator‐associated pneumonia (VAP). Therefore, the aim of current study is to describe critical care nurses’ knowledge and adherence to evidence‐base guidelines (EBGs) for preventing the occurrence of VAP.Methodology: A cross‐sectional study was applied. Data were collected through a self‐administered questionnaire completed by all critical care nurses (n = 213) working at Palestinian hospitals. Descriptive and inferential statistic was utilized to describe ICU nurse’s knowledge and adherence to VAP prevention EBGs.Results: The study revealed that the mean knowledge score for critical care nurses was (50.8%). Overall, the findings indicated that nurses’ knowledge of VAP guidelines was at an average level. A statistically significant difference (p value = 0.049) in the knowledge level was observed based on nurses’ qualifications. On the other hand, the study found that nurses’ adherence to EBGs was an acceptable (mean = 8.3, 69.2%). No substantial differences in adherence level were identified based on respondents’ characteristics.Conclusion: Critical care nurses possess an average level of knowledge regarding EBGs for preventing VAP, alongside demonstrating an acceptable level of adherence to these guidelines.
Clinical Correlates of Efficacy of Pyridostigmine in the Treatment of Orthostatic Hypotension
Authors: Okamoto Luis E. , Walsh Emily , Diedrich Andre , Shibao Cyndya A. , Gamboa Alfredo , Black Bonnie K. , Paranjape Sachin Y. , Muldowney James A. , Habermann Ralf , Peltier Amanda , Tarpara Kishan , Biaggioni Italo 
Published: December 2024

BACKGROUND:
The cholinesterase inhibitor pyridostigmine is used to treat orthostatic hypotension by facilitating cholinergic neurotransmission in autonomic ganglia, thereby harnessing residual sympathetic tone to increase blood pressure (BP) preferentially in the upright posture. We hypothesized that less severe autonomic impairment was associated with greater pressor responses to pyridostigmine.

METHODS:
To identify predictors of pressor response, linear regression analyses between the effect of pyridostigmine on upright BP and markers of autonomic impairment were retrospectively conducted on 38 patients who had a medication trial with pyridostigmine (60 mg single dose).

RESULTS:
Pyridostigmine increased upright BP by 4±2/3±2 mm Hg but with a wide range of responses (−20/−15 to 29/27 mm Hg; interquartile range, −6/−4 to 11/8 mm Hg). No differences were found between multiple system atrophy (n=14) and patients with pure autonomic failure (n=24). The upright BP response to pyridostigmine was negatively correlated with supine BP and with the pressure recovery time of the Valsalva maneuver, an index of severity of autonomic impairment. In patients with multiple system atrophy, the systolic blood pressure pressor response to pyridostigmine was also positively correlated with the increase in upright heart rate divided by the fall in systolic blood pressure (∆ heart rate/∆ systolic blood pressure) and with upright plasma norepinephrine, both surrogates of residual autonomic function.

CONCLUSIONS:
Patients with less severe autonomic impairment are more likely to have a positive pressor response to pyridostigmine. Importantly, in this cohort of patients with severe autonomic failure, pyridostigmine was not effective in those with supine hypertension who would benefit the most from the preferential pressor effect of the drug on upright BP.

REGISTRATION:

URL:
http://www.clinicaltrials.gov
; Unique identifier: NCT00223691.