bluuebunny/crossref_metadata_2025_split huggingface dataset:
Adriamycin-resistant cells are significantly less fit than adriamycin-sensitive cells in cervical cancer
Authors: Qi Min , Xie Lijuan , Duan Guihua 
Published: January 2021

Abstract
Adriamycin (ADR) is an important chemotherapy agent in many advanced cancers, but the emergence of drug resistance during treatment is a major limitation to its successful use. Recent studies have suggested that drug-resistant cells become less fit and their growth could be inhibited by parental cells without cytotoxic treatment. In this study, we examined the fitness differences between HeLa and HeLa/ADR cells. Compared with the parental cell line, HeLa/ADR cells showed significantly lower growth rates, both in vitro and in vivo. There was no difference in the apoptosis rate between them, but G1 arrest and reduced DNA synthesis were found in HeLa/ADR cells. Further study indicated that HeLa/ADR cells failed to compete for space and nutrition against parental cells in vivo. Taken together, we demonstrate that HeLa/ADR cells are less fit and their growth can be inhibited by parental cells in the absence of ADR; therefore, the maintenance of a certain amount of ADR-sensitive cells during treatment may facilitate the control of the development of ADR resistance.
Effects of inorganic and organic selenium sources on the growth performance of broilers in China: A meta-analysis
Authors: Wei Chunbo , Lin Xiuwei , Zhang Ying , Wan Xuanchen , Wu Haotong , He Tao , Bi Kai , Wang Changping 
Published: January 2021

Abstract
This study aims to investigate the effects of different selenium (Se) sources on the growth performance of Chinese broilers and provide a scientific rationale for adding Se additives to broiler feed. Relevant studies that meet standard inclusion criteria were identified and extracted from China National Knowledge Infrastructure and Wanfang and Chinese Scientific Journal (VIP) databases. A total of 9 studies with 539 subjects were included. A meta-analysis was performed with STATA15.0 to estimate the combined standardized mean difference (SMD) with a 95% confidence interval (95% CI). Heterogeneity test of articles was examined by Q-test, and the results showed that P values of feed conversion ratio, average daily gain (ADG), and average daily intake were all less than 0.05, suggesting a strong heterogeneity among the selected literature. Therefore, the random effect model is selected to calculate the SMD of the three indexes. The combined SMDs (95% CI) of feed:gain, ADG, and average daily feed intake (ADFI) were −0.39 (−1.03, 0.25), 0.26 (−0.29, 0.81), and −1.45 (−3.09, 0.20), respectively, and the P values were all less than 0.05. This study shows that the absolute differences in the growth performance (feed:gain, ADG, and ADFI) of broilers fed with either organic or inorganic Se supplements at the same dose were quite small. The P values of Egger’s test were 0.770, 0.089, and 0.426, respectively, for the above indexes, showing no significant publication bias. Sensitivity analysis ensured the stability and reliability of the results. In summary, the effects of organic and inorganic Se in feed on the growth performance of broilers are statistically equal.
Prorenin and active renin levels in paediatrics: a bioanalytical review
Authors: Burdman Ilja , Burckhardt Bjoern B. 
Published: January 2021

Abstract
As part of the extended renin-angiotensin-aldosterone system, active renin and its precursor prorenin have been an area of research interest for decades. Although several studies showed a correlation with disease, other studies found no significant association, e.g. attributed to limited sample size or pharmacological effects of antihypertensive drugs. Since the measurement of both proteins has typically been carried out in adult populations, the data in paediatrics is limited. This review aimed to collate the current data on plasma renin and prorenin levels in children and compare the levels of healthy vs. the diseased state. A literature search using Medline resulted in 213 publications of which 15 studies were classified as relevant. In the extant studies in the literature, an age-dependent decline of renin plasma concentration was observed in newborns compared to adolescents. For children with cardiovascular disease, five studies were identified that provide limited insight into the pathophysiological regulation of renin. In general, sample handling is still a crucial step, which might particularly affect measured active renin concentrations due to conformational changes of its precursor prorenin. A reliable assessment for prorenin levels in the maturating population is yet not possible due to the low number of available publications. Three different approaches to quantify prorenin were found and raise the question on the comparability of these methods. The review emphazised several weaknesses and highlights the need for an accurate procedure to determine levels of active renin as well as prorenin in its closed and open form.
Comparative study of i-SENS glucometers in neonates using capillary blood samples
Authors: Kim Ha Nui , Yoon Soo-Young 
Published: January 2021

Abstract

Objectives
The accuracy of point-of-care blood glucometers in the detection and evaluation of neonatal hypoglycemia is a concern. This study compared the performance of three i-SENS glucometers with that of the YSI 2300 STAT Plus Analyzer, which was used as a reference.

Methods
The leftover neonatal capillary blood samples of 319 patients were used in this study. The evaluation process and accuracy performance criteria were based on the International Organization for Standardization 15197:2013 guidelines. The evaluation involved three i-SENS glucometers (BAROzen H Expert plus, CareSens PRO, and CareSens H Beat) and the ACCU-CHEK® Inform II glucometer.

Results
The accuracy evaluation yielded acceptable results as follows: a) 100 and 100% for BAROzen H Expert plus; 99.8 and 100% for CareSens PRO; 98.7%, and 97.2% for CareSens H Beat glucometers were within the range of ±0.8 mmol/L (15 mg/dL) and ±15% of the average reference values at glucose concentrations <5.55 mmol/L (100 mg/dL) and ≥5.55 mmol/L (100 mg/dL), respectively, and b) all estimated glucose values (100%) were within the zones A and B of Consensus Error Grid for all three i-SENS glucometers. There was good correlation between the glucose values estimated by the glucometers and the reference values (R>0.990).

Conclusions
This study demonstrated that i-SENS glucometers exhibit acceptable performance and can be used as effective point-of-care devices in neonates.
Evaluation of dried blood spots as alternative sampling material for serological detection of anti-SARS-CoV-2 antibodies using established ELISAs
Authors: Weisser Heike , Steinhagen Katja , Höcker Ralf , Borchardt-Lohölter Viola , Anvari Özlem , Kern Peter M. 
Published: January 2021

Abstract

Objectives
During the current pandemic, antibody testing based on venous serum helps to determine whether the tested person has been previously infected with SARS-CoV-2. Alternatively, capillary blood can be taken via a finger prick (dried blood spots, DBS). In this study, paired DBS and venipuncture samples were tested using two serological assays to evaluate the usability of DBS for the detection of anti-SARS-CoV-2 antibodies.

Methods
Paired samples of DBS and venous serum were collected from 389 volunteers, of whom 75 had a recent PCR-confirmed SARS-CoV-2 infection, and tested for anti-SARS-CoV-2 IgG antibodies against both viral S1 and nucleocapsid protein (NCP) antigens using two ELISAs. Degree of agreement and correlation coefficients between ELISA results based on the two sampling methods were calculated.

Results
Results of DBS showed almost perfect agreement and high correlations with results from corresponding serum samples in both the S1-based ELISA and the NCP-based ELISA.

Conclusions
ELISA results derived from DBS showed very high agreement to those obtained with serum, supposing adequate usability and robustness of DBS as sample material for detection of anti-SARS-CoV-2 antibodies. In the near future, large-scale epidemiological screening for antibodies against SARS-CoV-2 will be carried out. Since DBS reduce the strain on healthcare institutions regarding sample collection, they have a potential to facilitate efficient community- and population-based screening in the current SARS-CoV-2 pandemic.
The underestimated issue of non-reproducible cardiac troponin I and T results: case series and systematic review of the literature
Authors: Favresse Julien , Bayart Jean-Louis , Gruson Damien , Bernardini Sergio , Clerico Aldo , Perrone Marco 
Published: January 2021

Abstract
Cardiac troponins (cTn) are the preferred biomarkers for the evaluation of myocardial injury and play a key role in the diagnosis of acute myocardial infarction (MI). Pre-analytical or analytical issues and interferences affecting troponin T and I assays are therefore of major concern given the risk of misdiagnosis. False positive troponin results have been related to various interferences including anti-troponin antibodies, heterophilic antibodies, or elevated alkaline phosphatase level. On the other hand, false negative results have been reported in the case of a large biotin intake. These interferences are characterized with erroneous but reproducible troponin results. Of interest, non-reproducible results have also been reported in the literature. In other words, if the sample is reanalyzed a second time, a significant difference in troponin results will be observed. These interferences have been named “fliers” or “outliers”. Compared to the biotin interference that received major attention in the literature, troponin outliers are also able to induce harmful clinical consequences for the patient. Moreover, the prevalence of outliers in recent studies was found to be higher (0.28–0.57%) compared to the biotin interference. The aim of this systematic review is to warn clinicians about these non-reproducible results that may alter their clinical judgment. Four case reports that occurred in the Clinique of Saint-Luc Bouge are presented to attest this point. Moreover, we aimed at identifying the nature of these non-reproducible troponin results, determining their occurrence, and describing the best way for their identification.
Vitamin-D levels and intensive care unit outcomes of a cohort of critically ill COVID-19 patients
Authors: Orchard Laurence , Baldry Matthew , Nasim-Mohi Myra , Monck Chantelle , Saeed Kordo , Grocott Michael P. W. , Ahilanandan Dushianthan 
Published: January 2021

Abstract

Objectives
The pattern of global COVID-19 has caused many to propose a possible link between susceptibility, severity and vitamin-D levels. Vitamin-D has known immune modulatory effects and deficiency has been linked to increased severity of viral infections.

Methods
We evaluated patients admitted with confirmed SARS-COV-2 to our hospital between March-June 2020. Demographics and outcomes were assessed for those admitted to the intensive care unit (ICU) with normal (>50 nmol/L) and low (<50 nmol/L) vitamin-D.

Results
There were 646 SARS-COV-2 PCR positive hospitalisations and 165 (25.5%) had plasma vitamin-D levels. Fifty patients were admitted to ICU. There was no difference in vitamin-D levels of those hospitalised (34, IQR 18.5–66 nmol/L) and those admitted to the ICU (31.5, IQR 21–42 nmol/L). Higher proportion of vitamin-D deficiency (<50 nmol/L) noted in the ICU group (82.0 vs. 65.2%). Among the ICU patients, low vitamin D level (<50 nmol/L) was associated with younger age (57 vs. 67 years, p=0.04) and lower cycle threshold (CT) real time polymerase chain reaction values (RT-PCR) (26.96 vs. 33.6, p=0.02) analogous to higher viral loads. However, there were no significant differences in ICU clinical outcomes (invasive and non-invasive mechanical ventilation, acute kidney injury and mechanical ventilation and hospital days) between patients with low and normal vitamin-D levels.

Conclusions
Despite the association of low vitamin-D levels with low CT values, there is no difference in clinical outcomes in this small cohort of critically ill COVID-19 patients. The complex relationship between vitamin-D levels and COVID-19 infection needs further exploration with large scale randomized controlled trials.
Serum N-glycan fingerprint nomogram predicts liver fibrosis: a multicenter study
Authors: Huang Chenjun , Liu Lijuan , Wang Hao , Fang Meng , Feng Huijuan , Li Ya , Wang Mengmeng , Tong Lin , Xiao Xiao , Wang Ziyi , Xu Xuewen , He Yutong , Gao Chunfang 
Published: January 2021

Abstract

Objectives
Liver cirrhosis (LC) is the end-stage of fibrosis in chronic liver diseases, non-invasive early detection of liver fibrosis (LF) is particularly essential for therapeutic decision. Aberrant glycosylation of glycoproteins has been demonstrated to be closely related to liver abnormalities.

Methods
This study was designed to enroll a total of 1,565 participants with LC/LF, chronic hepatitis virus (CHB) and healthy controls. Fibrosis was confirmed by liver biopsy. Using capillary electrophoresis N-glycan fingerprint (NGFP) analysis, we developed a nomogram algorithm (FIB-G) to discriminate LC from non-cirrhotic subjects.

Results
The FIB-G demonstrated good diagnostic performances in identifying LC with the area under the curve (AUC) 0.895 (95%CI: 0.857–0.915). Furthermore, the diagnostic efficiencies of FIB-G were superior to that of log (P2/P8), procollagen III N-terminal (PIIINP), type IV collage (IV-C), laminin (LN), hyaluronic acid (HA), aspartate transaminase to platelets ratio index (APRI), and FIB-4 when detecting significant fibrosis (S0–1 vs. S2–4, AUC: 0.787, 95%CI: 0.701–0.873), severe fibrosis (S0–2 vs. S3–4, AUC: 0.844, 95%CI: 0.763–0.924), and LC (S0–3 vs. S4, AUC: 0.773, 95%CI: 0.667–0.880). Besides, changes of FIB-G were associated well with the regression of fibrosis and liver function Child–Pugh classification.

Conclusions
FIB-G is an accurate multivariant N-glycomic algorithm for LC prediction and fibrosis progression/regression monitoring. The high throughput feasible NGFP using only 2 μL of serum could help physicians make the more precise non-invasive staging of LF or cirrhosis and reduce the need for invasive liver biopsy.
Mechanism of bilirubin elimination in urine: insights and prospects for neonatal jaundice
Authors: Thomas Mercy , Hardikar Winita , Greaves Ronda F. , Tingay David G. , Loh Tze Ping , Ignjatovic Vera , Newall Fiona , Rajapaksa Anushi E. 
Published: January 2021

Abstract
Despite a century of research, bilirubin metabolism and the transport mechanisms responsible for homeostasis of bilirubin in serum remain controversial. Emerging evidence on the hepatic membrane transporters and inherited disorders of bilirubin metabolism have contributed to a greater understanding of the various steps involved in bilirubin homeostasis and its associated excretory pathways. We discuss these recent research findings on hepatic membrane transporters and evaluate their significance on the newborn bilirubin metabolism and excretion. New insights gained speculate that a proportion of conjugated bilirubin is excreted via the renal system, as an alternative to the intestinal excretion, even in normal physiological jaundice with no associated pathological concerns. Finally, this paper discusses the clinical relevance of targeting the altered renal excretory pathway, as bilirubin in urine may hold diagnostic importance in screening for neonatal jaundice.
How to meet ISO15189:2012 pre-analytical requirements in clinical laboratories? A consensus document by the EFLM WG-PRE
Authors: Vermeersch Pieter , Frans Glynis , von Meyer Alexander , Costelloe Seán , Lippi Giuseppe , Simundic Ana-Maria 
Published: January 2021

Abstract
The International Organization for Standardization (ISO) 15189:2012 standard aims to improve quality in medical laboratories through standardization of all key elements in the total testing process, including the pre-analytical phase. It is hence essential that accreditation bodies, assessing laboratories against ISO15189:2012, pay sufficient attention to auditing pre-analytical activities. However, there are significant differences in how technical auditors interpret the pre-analytical requirements described in ISO15189:2012. In this consensus document, the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM) Working Group for Pre-analytical Phase (WG-PRE) sets out to review pre-analytical requirements contained in ISO15189:2012 and provide guidance for laboratories on how to meet these requirements. The target audience for this consensus document is laboratory professionals who wish to improve the quality of the pre-analytical phase in their laboratory. For each of the ISO requirements described in ISO15189:2012, members of EFLM WG-PRE agreed by consensus on minimal recommendations and best-in-class solutions. The minimal consensus recommendation was defined as the minimal specification which laboratories should implement in their quality management system to adequately address the pre-analytical requirement described in ISO15189:2012. The best-in-class solution describes the current state-of-the-art in fulfilling a particular pre-analytical requirement in ISO15189:2012. We fully acknowledge that not every laboratory has the means to implement these best-in-class solutions, but we hope to challenge laboratories in critically evaluating and improving their current procedures by providing this expanded guidance.
Chinese does have independent tense elements
Authors: Zhang Zhiyi , Shikun Li 
Published: January 2021

AbstractPrevious research on Chinese tense indicates that Chinese has either null tense or no tense. However, the present study suggests that the conclusions of previous studies regarding Chinese tense are either against the syntactic truth or illogical. The present study provides new evidence to support that Chinese has two independent tense elements,zaiandguo, which clearly indicate present and past tense, contrary to the traditional assumption that they are aspectual markers. From the perspective of grammaticalization, bothzaiandguowitnessed grammaticalization from the spatial concept to the temporal concept. The semantic evidence shows thatzaiandguoare semantically different from the aspectual markerszheandleand convey the meaning of time location. The fact that bothzaiandguoare allowed in negation but not permitted in non-finite structure provides syntactic evidence that they are tense markers. However, the present study also suggests there can be two differentzaiandguo;zaiandguoused separately and independently andzaiandguoused withzheandle.In the latter case,zaiis a time adverbial andguois an aspectual marker. The existence of independent tense markers in Chinese also shows that Chinese may have at least four different mechanisms to anchor tense.
A multimodal analysis of Jane Eyre book covers
Authors: Wu Jie , Wu Qinghong 
Published: January 2021

Abstract
This paper applies visual grammar theory to make a multimodal discourse analysis of book covers of the different Chinese versions of Jane Eyre. It illustrates the relationship between the social environment and social changes in Chinese society and culture in relation to an increasing social acceptance of the novel in China. This research not only validates the applicability and practicability of visual grammar in analyzing book covers, but also helps to show an ideological change in Chinese readers and publishers over time from the 1930s to the 21st century. Indeed, the connotations of book covers of the different Chinese versions of Jane Eyre seem to be closely related to different historical and social contexts. They document and bear witness to, in their particular way, the tremendous changes in Chinese society from early last century on. They also show that Jane, the heroine of the novel, seemed to be re-discovered over and over again in China, depending on the main social features of different periods. She seems to have been portrayed as the gray presence, the feminist, the lover and protagonist, and finally the icon.
Double consciousness to scaffold narrative skills
Authors: West Donna E. 
Published: January 2021

Abstract
Determining the utility of auditory hallucinations (including imaginary friends) in developing logic is sorely under-investigated (Fernyhough, Charles. 2016. The voices within: The history and science of how we talk to ourselves. New York: Basic Books). The present account demonstrates how Peirce’s double consciousness fueled by his endoporeutic principle, provides insight into how abduction directs adopting arguments from one source while dismissing others. Peirce’s categories provide hints as to which voices become admitted to logical scrutiny, and which are validated – consequent to irritations imposed by surprise/conflict. Effort/resistance (4.536) clearly illustrates how Secondness legitimizes emerging perspectives, facilitating examination of peripheral voices, which can be competitive (MS 9) or collaborative (4.551). Peirce’s Energetic and Emotional Interpretants (MS 318) impel or inhibit new habits (attention to one stimulus over another). Consciously inhibiting forces hastens self-control (Thirdness) integrating voices on the fringes of conventionality into one’s own (MS 318). Ultimately, incorporating alterity via imagined arguments satisfies Peirce’s endoporeutic maxim because reflecting upon the legitimacy of alien perspectives transforms habits from the outside in.
Peirce on learning and teaching
Authors: Nöth Winfried 
Published: January 2021

Abstract
The paper is a precis of C. S. Peirce’s semiotic theory of education. It presents this theory of learning and teaching from the perspective of Peirce’s phenomenological categories of Firstness, Secondness, and Thirdness. In the domain of Thirdness, learning is mediation between ignorance and knowledge, new information and old knowledge. Teaching has its focus on laws, symbols, legisigns, and reasoning. In the domain of Secondness, learners acquire new knowledge from the “hard realities” of real-life experience, from obstacles, and from the resistance caused by error and doubt. Teaching takes place by means of sinsigns (singular signs) and indexical signs. In the domain of Firstness, the learner acquires familiarity with the sensory qualities of objects of experience and learns from free associations, imagination, and acts of creativity. The instruments of teaching are qualisigns, icons, and abductive reasoning. The paper concludes that Peirce’s philosophy of education is holistic insofar as it states that most efficient signs are those signs in which “the iconic, indicative, and symbolic characters are blended as equally as possible.”