bluuebunny/crossref_metadata_2025_split huggingface dataset:
Sweet impersonators: Molecular mimicry of host glycans by bacteria
Authors: de Jong Hanna , Wösten Marc M S M , Wennekes Tom 
Published: October 2021

Abstract
All bacteria display surface-exposed glycans that can play an important role in their interaction with the host and in select cases mimic the glycans found on host cells, an event called molecular or glycan mimicry. In this review, we highlight the key bacteria that display human glycan mimicry and provide an overview of the involved glycan structures. We also discuss the general trends and outstanding questions associated with human glycan mimicry by bacteria. Finally, we provide an overview of several techniques that have emerged from the discipline of chemical glycobiology, which can aid in the study of the composition, variability, interaction and functional role of these mimicking glycans.
Integrating student and teacher insights to a school-based alcohol program through co-design
Authors: Durl James , Dietrich Timo , Williams Ben , Rundle-Thiele Sharyn 
Published: October 2021

Summary
School-based programs may be more effective when an array of stakeholders, including users, are involved during the relevant stages of program co-creation—specifically during key development stages such as design, implementation and evaluation. How such programs can be operationally co-created and tested is less known and is therefore the purpose of this article. Two sequential co-design phases underpin this study. First, a co-design session with 20 health and physical education (HPE) teachers focussed on reviewing, testing and critically discussing initial prototype online modules for an alcohol education program. Teacher insights were assessed and incorporated, and the updated online modules were tested with secondary school students (n = 120) capturing their experience in a classroom setting. Insights from each group were analysed using thematic analysis. Teachers and students serve important roles in program co-creation. Teachers remain an underutilized stakeholder group whom are however critical in delivering important insights to enhance educational program design. Teachers demanded more relevance to the national curriculum, further alignment with curriculum assessment standards, and age appropriate content. Student feedback focussed largely on realistic content, personalization and gamification elements. This study represents an application of the seven-step co-design process and advances understanding of the ‘fuzzy back-end’ of the process, namely reflecting on feasibility of integrating teacher and student feedback and ideas. More specifically, how these key—yet distinct—stakeholder groups can be involved, and the merits of their involvement are discussed.
The quest for successful Atlantic salmon restoration: perspectives, priorities, and maxims
Authors: Lennox Robert J , Alexandre Carlos M , Almeida Pedro R , Bailey Kevin M , Barlaup Bjørn T , Bøe Kristin , Breukelaar André , Erkinaro Jaakko , Forseth Torbjørn , Gabrielsen Sven-Erik , Halfyard Edmund , Hanssen Erlend M , Karlsson Sten , Koch Stephanie , Koed Anders , Langåker Roy M , Lo Håvard , Lucas Martyn C , Mahlum Shad , Perrier Charles , Pulg Ulrich , Sheehan Timothy , Skoglund Helge , Svenning Martin , Thorstad Eva B , Velle Gaute , Whoriskey Frederick G , Vollset Knut Wiik 
Published: October 2021

AbstractAtlantic salmon is often a focal species of restoration efforts throughout the north Atlantic and it is therefore an excellent case study for how best to design programmes to address and mitigate threats and correct population declines. This perspective is written to promote the work that has been accomplished towards restoration of Atlantic salmon populations and synthesize how we believe the lessons can be used effectively to support efforts by management agencies to restore populations. We reviewed where restoration is needed for Atlantic salmon, agreed on definitions for three levels of successful restoration, and then applied these criteria to 49 published papers focused on Atlantic salmon restoration. We identified 16 successful examples of restoration among 49 papers reviewed and discussed what interventions led to success versus failure. We then addressed key questions about when hatchery stocking should be used as part of a restoration measure and whether local restoration efforts are enough when these wide-ranging species encounter broad-scale changes in the north Atlantic, specifically related to issues of climate change and to marine survival. We advise to avoid restoration as much as possible by protecting and managing existing populations and when restoration is necessary, problems should be identified and addressed in partnership with river users. With appropriate resources and research to resolve ongoing mysteries, restoration of lost Atlantic salmon populations is absolutely feasible.
A framework for assessing the skill and value of operational recruitment forecasts
Authors: Kiaer Christian , Neuenfeldt Stefan , Payne Mark R 
Published: October 2021

Abstract
Forecasting variation in the recruitment to fish stocks is one of the most challenging and long-running problems in fisheries science and essentially remains unsolved today. Traditionally, recruitment forecasts are developed and evaluated based on explanatory and goodness-of-fit approaches that do not reflect their ability to predict beyond the data on which they were developed. Here, we propose a new generic framework that allows the skill and value of recruitment forecasts to be assessed in a manner that is relevant to their potential use in an operational setting. We assess forecast skill based on predictive power using a retrospective forecasting approach inspired by meteorology, and emphasize the importance of assessing these forecasts relative to a baseline. We quantify the value of these forecasts using an economic cost-loss decision model that is directly relevant to many forecast users. We demonstrate this framework using four stocks of lesser sandeel (Ammodytes marinus) in the North Sea, showing for the first time in an operationally realistic setting that skilful and valuable forecasts are feasible in two of these areas. This result shows the ability to produce valuable short-term recruitment forecasts, and highlights the need to revisit our approach to and understanding of recruitment forecasting.
Breast cancer risk in residents of Belarus exposed to Chernobyl fallout while pregnant or lactating: standardized incidence ratio analysis, 1997 to 2016
Authors: Cahoon Elizabeth K , Preston Dale , Zhang Rui , Vij Vibha , Little Mark P , Mabuchi Kiyohiko , Drozdovitch Vladimir , Chizhov Konstantin , Yauseyenka Vasilina V , Rozhko Alexander V , Velalkin Ilya V 
Published: October 2021

Abstract

Background
Pregnancy and lactation may constitute radiation-sensitive reproductive periods due to rapid cell proliferation and concentration of radioiodine in the lactating breast. However, there are limited epidemiological data among women exposed to radiation during these periods.

Methods
We examined incidence of breast cancer in a cohort of 3214 women who were pregnant and/or lactating within 2 months (26 April to 30 June 1986) of the Chernobyl accident and residing in contaminated regions of Belarus. There were 33 breast cancers identified through linkage with the Belarusian National Cancer Registry. Breast cancer rates among women who were either pregnant and/or lactating were compared with population rates by calculating standardized incidence ratios (SIR) and 95% confidence intervals (CI) adjusting for attained age, oblast, urban/rural residence and calendar year.

Results
Among women who were lactating, we found a greater than 2-fold increased risk of breast cancer compared with the general population, SIR = 2.49 (95% CI: 1.55, 3.75). In contrast, women who were pregnant were not at increased risk (SIR = 0.84 95% CI: 0.46, 1.38). The SIR was highest in women who were exposed at a younger age and at the earliest time period since the accident, though stratified analyses had limited sample sizes.

Conclusions
We found a significantly increased risk of breast cancer among women residing in contaminated areas of Belarus who were lactating at the time of the accident and may have had elevated exposure to radioiodine, when compared with the general population. Studies of breast cancer with individual radiation dose estimates among women exposed during lactation are warranted.
Time Taken to Detect and Respond to Polio Outbreaks in Africa and the Potential Impact of Direct Molecular Detection and Nanopore Sequencing
Authors: Shaw Alexander G , Cooper Laura V , Gumede Nicksy , Bandyopadhyay Ananda S , Grassly Nicholas C , Blake Isobel M 
Published: October 2021

Abstract

Background
Detection of poliovirus outbreaks relies on a complex laboratory algorithm of cell-culture, polymerase chain reaction (PCR), and sequencing to distinguish wild-type and vaccine-derived polioviruses (VDPV) from Sabin-like strains. We investigated the potential for direct molecular detection and nanopore sequencing (DDNS) to accelerate poliovirus detection.

Methods
We analyzed laboratory data for time required to analyze and sequence serotype-2 VDPV (VDPV2) in stool collected from children with acute flaccid paralysis in Africa (May 2016–February 2020). Impact of delayed detection on VDPV2 outbreak size was assessed through negative binomial regression.

Results
VDPV2 confirmation in 525 stools required a median of 49 days from paralysis onset (10th–90th percentile, 29–74), comprising collection and transport (median, 16 days), cell-culture (7 days), intratypic differentiation quantitative reverse transcription PCR (3 days), and sequencing, including shipping if required (15 days). New VDPV2 outbreaks were confirmed a median of 35 days (27–60) after paralysis onset, which we estimate could be reduced to 16 days by DDNS (9–37). Because longer delays in confirmation and response were positively associated with more cases (P &lt; .001), we estimate that DDNS could reduce the number of VDPV2 cases before a response by 28% (95% credible interval, 12%–42%).

Conclusions
DDNS could accelerate poliovirus outbreak response, reducing their size and the cost of eradication.
Sex Differences in How Territory Quality Affects Aggression in Convict Cichlids
Authors: Leese Joseph M , Blatt T 
Published: October 2021

Synopsis
In animal contests, the value an individual assigns to limited resources can directly impact the level of aggression it demonstrates. For territorial species, individuals often assess their territory quality and appropriately modify the time and energy invested in its defense. In this study, male and female convict cichlids, Amatitlania nigrofasciata, were acclimated to one of three territorial treatments representing either a low, medium, or high resource value. Territories with a “Low Value” included substrate alone, “Medium Value” territories included substrate and a nest site, and a “High Value” territory included substrate, a nest site, and constant food source. After three days of acclimation, a size-matched intruder was introduced to elicit territorial aggression and behaviors were observed. Territory quality affected one measure of low-intensity aggression (displays) in residents but had no effect on high-intensity aggression (bites and chases). Moreover, there was a significant effect of sex, with males and females differing in the types of aggressive behaviors demonstrated across all treatments. Females showed more low-intensity aggressive behaviors toward intruders than males did. Additionally, a significant interaction of sex and territory quality was observed on two measures of high-intensity aggressive behavior (bites and chases), with females more likely than males to increase aggressive behaviors along with increasing territory quality. This suggests that females may be more sensitive and/or responsive to changes in the quality of a territory, possibly due to the necessity of a suitable nest site for egg deposition within a territory.
Turtling the Salamander: Tail Movements Mitigate Need for Kinematic Limb Changes during Walking in Tiger Salamanders (Ambystoma tigrinum) with Restricted Lateral Movement
Authors: Vega Christine M , Ashley-Ross Miriam A 
Published: October 2021

Synopsis
Lateral undulation and trunk flexibility offer performance benefits to maneuverability, stability, and stride length (via speed and distance traveled). These benefits make them key characteristics of the locomotion of tetrapods with sprawling posture, with the exception of turtles. Despite their bony carapace preventing lateral undulations, turtles are able to improve their locomotor performance by increasing stride length via greater limb protraction. The goal of this study was to quantify the effect of reduced lateral flexibility in a generalized sprawling tetrapod, the tiger salamander (Ambystoma tigrinum). We had two potential predictions: (1) either salamanders completely compensate by changing their limb kinematics, or (2) their performance (i.e., speed) will suffer due to the reduced lateral flexibility. This reduction was performed by artificially limiting trunk flexibility by attaching a 2-piece shell around the body between the pectoral and pelvic girdles. Adult tiger salamanders (n = 3; SVL = 9–14.5 cm) walked on a 1-m trackway under three different conditions: unrestricted, flexible shell (Tygon tubing), and rigid shell (PVC tubing). Trials were filmed in a single, dorsal view, and kinematics of entire midline and specific body regions (head, trunk, tail), as well as the fore and hind limbs, were calculated. Tygon individuals had significantly higher curvature than both PVC and unrestricted individuals for the body, but this trend was primarily driven by changes in tail movements. PVC individuals had significantly lower curvature in the trunk region compared with unrestricted individuals or Tygon; however, there was no difference between unrestricted and Tygon individuals suggesting the shells performed as expected. PVC and Tygon individuals had significantly higher curvature in the tails compared with unrestricted individuals. There were no significant differences for any limb kinematic variables among treatments including average, minimum, and maximum angles. Thus, salamanders respond to decreased lateral movement in their trunk by increasing movements in their tail, without changes in limb kinematics. These results suggest that tail undulations may be a more critical component to sprawling-postured tetrapod locomotion than previously recognized.
Overcoming technical and cultural challenges to delivering equitable care for LGBTQ+ individuals in a rural, underserved area
Authors: Marney Heather L , Vawdrey David K , Warsame Leyla , Tavares Spencer , Shapiro Andrea , Breese Arthur , Brayford Amy , Chittalia Aliasgar Z 
Published: October 2021

Abstract
The lesbian, gay, bisexual, transgender, queer, or questioning (LGBTQ+) community is vulnerable to health-care disparities. Many health-care organizations are working to collect sexual orientation and gender identity in their electronic health records (EHRs), with the goal of providing more inclusive care to their LGBTQ+ patients. There are significant human and technical barriers to making these efforts successful. Based on our 5-year experience at Geisinger (an integrated health system located in a rural, generally conservative area), this case report provides insights to overcome challenges in 4 critical areas: (1) enabling the EHR to collect and use information to support the health-care needs of LGBTQ+ patients, (2) building a culture of awareness and caring, empowering members of the health-care team to break down barriers of misunderstanding and mistrust, (3) developing services to support the needs of LGBTQ+ patients, and (4) partnering with local communities to become a trusted health-care provider.
A systematic review on natural language processing systems for eligibility prescreening in clinical research
Authors: Idnay Betina , Dreisbach Caitlin , Weng Chunhua , Schnall Rebecca 
Published: October 2021

Abstract

Objective
We conducted a systematic review to assess the effect of natural language processing (NLP) systems in improving the accuracy and efficiency of eligibility prescreening during the clinical research recruitment process.

Materials and Methods
Guided by the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) standards of quality for reporting systematic reviews, a protocol for study eligibility was developed a priori and registered in the PROSPERO database. Using predetermined inclusion criteria, studies published from database inception through February 2021 were identified from 5 databases. The Joanna Briggs Institute Critical Appraisal Checklist for Quasi-experimental Studies was adapted to determine the study quality and the risk of bias of the included articles.

Results
Eleven studies representing 8 unique NLP systems met the inclusion criteria. These studies demonstrated moderate study quality and exhibited heterogeneity in the study design, setting, and intervention type. All 11 studies evaluated the NLP system’s performance for identifying eligible participants; 7 studies evaluated the system’s impact on time efficiency; 4 studies evaluated the system’s impact on workload; and 2 studies evaluated the system’s impact on recruitment.

Discussion
NLP systems in clinical research eligibility prescreening are an understudied but promising field that requires further research to assess its impact on real-world adoption. Future studies should be centered on continuing to develop and evaluate relevant NLP systems to improve enrollment into clinical studies.

Conclusion
Understanding the role of NLP systems in improving eligibility prescreening is critical to the advancement of clinical research recruitment.
Sublethal Effects of Abamectin on the Development, Reproduction, Detoxification Enzyme Activity, and Related Gene Expression of the Oriental Fruit Moth (Lepidoptera: Tortricidae)
Authors: Su Sha , Jian Chengzhi , Zhang Xiaohe , Fang Sensen , Peng Xiong , Piñero Jaime C , Chen Maohua 
Published: October 2021

Abstract
Grapholita molesta is one of the most important fruit pests worldwide. Abamectin is a biological pesticide frequently used to control fruit borers like G. molesta in part owing to its translaminar properties. In this study, we characterized the toxicity of abamectin to G. molesta larvae using the diet incorporation method. The sublethal effects of abamectin on the development, reproduction, detoxification enzyme activity, and related gene expression of G. molesta were assessed. The results showed that the LC20 and LC50 values of the insecticide against G. molesta 72 h post-treatment were 1.17 mg L–1 and 5.85 mg L–1, whereas the LC20 and LC50 values 96 h post-treatment were 0.34 mg L–1 and 3.63 mg L–1. When compared to the control, sublethal concentrations of abamectin 1) significantly increased the mortality of the larvae, prepupae, and pupae of G. molesta, 2) prolonged the duration of 3rd to 5th instar larva, prepupal and pupal periods, 3) shortened the longevity of adults, and 4) reduced female fecundity. The enzymatic activity of glutathione S-transferase (GST) varied significantly after exposure to sublethal concentrations of abamectin, but the cytochrome P450 monooxygenases and carboxylesterase activity were not significantly affected. Thirteen of the 25 GST genes were significantly upregulated under different sublethal concentrations of abamectin. The combined findings increase our understanding of the effects of abamectin on G. molesta and the potential role of GSTs in the metabolic interactions of abamectin in this pest, and have applications for more rational and effective use of abamectin to control G. molesta.
Endovascular treatment of a large saccular aneurysm of the celiac artery: a case report and review of literature
Authors: Salimi Javad , Mangouri Amir , Baradaran Ali Farahanchi 
Published: October 2021

Abstract
Celiac artery is a visceral abdominal vasculature whose aneurysms are very rare, accounting for less than 0.01% of all aneurysms. This condition can be treated by open aneurysmectomy or aneurysmorrhaphy and endovascular intervention. Due to the high mortality and morbidity associated with open surgery, endovascular intervention may be a better treatment option. Here, we present a case related to a 40-year-old man who had been experiencing vague epigastric pain for 4 months prior to admission and was managed endovascularly.
IgM Warm Autoantibodies Causing Autoimmune Hemolytic Anemia in a Pediatric Patient
Authors: Fortes Precious , Baez Janet , McGonigle Andrea M , Ziman Alyssa , Federman Noah , Ward Dawn C 
Published: October 2021

Abstract
Most often, IgM-mediated autoimmune hemolytic anemia (AIHA) presents as cold agglutinin disease in the pediatric population. The IgM warm agglutinins are rare, with few reports in the literature. This case study describes a 5 year old girl with nausea, abdominal pain and jaundice, and a hemoglobin of 5.5 g/dL who was diagnosed with a warm reactive IgM AIHA. The laboratory workup revealed a pan-reactive antibody and a direct antiglobulin test negative for IgG and C3. A thermal amplitude assay revealed reactive IgM antibodies at 37°C, 30°C, 25°C, and 4°C and an antibody titer of 1:8. An adsorption for IgM-specific autoantibodies exposed underlying anti-E and anti-Cw alloantibodies. Transfusion of phenotypically matched red blood cell units supported ongoing hemolysis. The AIHA treatment included steroids followed by rituximab with complete resolution. A literature review shows variable outcomes for warm AIHA in the pediatric population and often describes the presence of warm reactive IgM-mediated AIHA as an indicator for poor prognosis.
Chest Pain in the Deployed Setting: A Case of Wellens Syndrome
Authors: Mozeleski Eric M , Callahan Travis A , Vande Hei Anthony G 
Published: October 2021

ABSTRACT
Managing patients with chest pain in deployed and austere environments can be extremely challenging for military health care providers. Limited resources, including access to equipment, emergent cardiac catheterization, and cardiac surgery, can lead to deleterious consequences, including myonecrosis and possible death. Wellens syndrome is a form of acute coronary syndrome that involves stenosis of the left anterior descending coronary artery, leading to possible acute myocardial infarction. Most cases in the literature involve the diagnosis and treatment of Wellens syndrome in the civilian, nondeployed hospital setting. We present a case of a civilian contractor who had experienced an episode of chest pain that had resolved with rest and now had new findings on electrocardiogram concerning Wellens syndrome.