bluuebunny/crossref_metadata_2025_split huggingface dataset:
C-Reactive Protein Knock Out Attenuate Temporomandibular Joint Inflammation in Rats
Authors: He Yao , Zhou Mengjiao , Jian Zixiang , Fang Lingli , Huang Lan , Song Jinlin 
Published: September 2021

Abstract
Background: C-reactive protein (CRP), a biomarker of inflammation, is highly expressed in osteoarthritis (OA)-related diseases, but its exact role remains unknown. In this study, we evaluated the biological effect of CRP on temporomandibular joint (TMJ) inflammation.Methods: Freund’s complete adjuvant (CFA) was used to induce TMJ inflammation in CRP-knockout (CRP-/-) and control rats. Degenerative changes in the TMJ were compared to elucidate the role of CRP in TMJ inflammation. In addition, inflammatory cytokines, macrophage activation and osteoclast differentiation were evaluated by real-time quantitative polymerase chain reaction, immunohistochemistry and tartrate-resistant phosphatase staining to explore the potential regulatory mechanism.Results: Compared to the control, CFA induced TMJ inflammation, which increased systemic and local CRP expression. Furthermore, CRP-/- rats exhibited less severe inflammatory symptoms. The downregulation of proinflammatory cytokines (interleukin (IL)-1β and IL-6) and upregulation of the anti-inflammatory cytokine IL-10 were detected in CRP-/- rats, which also exhibited reduced macrophage activation and osteoclast differentiation.Conclusion: These results indicated that controlling the highly elevated levels of CRP during inflammation could modify the cytokine profile, macrophage activation and osteoclast differentiation, thus providing beneficial effects for TMJ-OA prevention and treatment.
A Validation Study of The Greek Version of The Toronto Empathy Questionnaire In Medical Students And A Measurement of Their Empathy
Authors: Voultsos Polychronis , Chatzinikolaou Fotios , Papana Angeliki , Deliliga Aspasia 
Published: September 2021

Abstract
Aims and objectives: Empathy is an important key driver of any therapeutic relationship. It is beneficial not only to the patients, but also to physicians. Enhancing physician’s empathy should be an important goal of medical education. As there is a literature gap regarding the topic of empathy among medical students in Greece, this study aims to contribute to filling this gap.Methods: A cross-sectional study was conducted. The (validated in Greece) Greek version of Toronto 52‐item empathy 6-point Likert-scale was administered to all the medical students in the Aristotle University of Thessaloniki, in Greece. In addition, participants were asked to provide information regarding their socio-demographics. A demographic comparison was conducted.Results: The preliminary validation of the Greek version of the Toronto Composite Empathy Scale (TCES) demonstrated acceptable validity and reliability among medical students and could be further tested in larger samples of medical students. The overall reliability analysis of the TCES questionnaire is high (Cronbach's α = 0.895, Sig. from Hotelling’s T-Squared Test < 0.000). The mean total score of empathy showed that students have a moderately high empathy. The 52‐item TCES, 26 for the personal (Per) setting and another 26 for professional (Pro) life, equally divided into cognitive (Cog) and emotional (Emo) empathy in each case. It was found that there is a statistically significant difference in means between the Per-Cog and Per-Emo settings (Sig < 0.001), the Pro-Cog and Pro-Emo (Sig < 0.001), the Per-Cog and Pro-Cog (Sig = 0.004), and the Per-Emo and Pro-Emo (Sig < 0.001). Females had significantly higher empathy scores (mean score 208.04) than males (mean score 192.5) on the Per-Cognitive, Per-Emo and Pro-Emo subscales. Furthermore, a positive correlation was found between empathy and factors such as love for animals, interest in medical ethics, belief in God, having an ill person in the family, class year or carrier intention. Conclusions: The Toronto Composite Empathy Scale (TCES) is applicable to medical students. For the most part our findings were consistent with previous literature. However, we identified some nuances that might draw researchers’ attention.
Human Recombinant Leptin Shows Dose and Time-Dependent Release of Nitric Oxide from Endothelial Nitric Oxide Synthase in Endothelium While on Angiogenesis
Authors: Manjunathan Reji , Sinha Swaraj , Swaminathan Akila , Kasiviswanathan Dharanibalan , Ragunathan Malathi , Chatterjee Suvro 
Published: September 2021

Abstract
Nitric Oxide (NO) modulates various assortments of the angiogenic process. The endogenous hormone leptin is able to induce different physiological process such as angiogenesis at low concentration because of its high receptor specific affinity. Various studies speculated leptin’s ability to induce endothelium‐dependent vascular relaxation by stimulating NO through different signaling pathways. So far, no studies have reported the dose and time dependent potential of human recombinant leptin on NO release. Hence, an attempt has been made to understand the optimal concentration and time of incubation of human recombinant leptin for the enzymatic release of NO from endothelial Nitric Oxide Synthase (eNOS). Leptin induced changes in the localization and phosphorylation pattern of eNOS in cultured endothelium under various concentrations and time of incubation is studied. The 5 Nanomolar concentration of human recombinant leptin within 6 minutes of incubation could induce significant levels of NO from the activated eNOS in cultured endothelium through plasma membrane localization and phosphorylation of eNOS. Our findings suggest that human recombinant leptin can modulate NO-dependent new therapeutic avenue for angiogenesis-related disorders such as wound healing if used within the active concentration and time of incubation.
A Fuzzy MADM Approach for Selecting the Best Cyanide Removal Method from a Gold Mine Wastewater
Authors: Hayati Mohammad , Ganji Seyed Mohammad Seyed Alizadeh , Shahcheraghi Seyed Hadi 
Published: September 2021

Abstract
The cyanidation process is the most common method applied for the extraction of gold and silver in the hydrometallurgy industry, in which, sodium cyanide is used as a leaching agent. Therefore, the wastewater of gold mines contains a wide variety of cyanide ions needing to be removed before these wastewaters can be discharged to the receiving environments. In this study, a fuzzy multi-attribute decision-making approach (Fuzzy Delphi AHP and Fuzzy TOPSIS) was used for selecting the best cyanide removal method from the wastewater of Muteh gold mine. According to the experts' opinion, three methods including calcium hypochlorite, hydrogen peroxide and sodium hypochlorite were selected as alternatives. Then, by introducing the criteria influencing decision making, including cyanide removal ability, cost of process, amount of material consumed, time, pH, ease of performance and safety, and performing separated experiments, the criteria for each of three methods were determined. Finally, sodium hypochlorite was proposed as the best method for eliminating cyanide from wastewater. It was found that the rank of methods was as sodium hypochlorite (0.517) > calcium hypochlorite (0.474) > hydrogen peroxide (0.463).
Stratifin Indicates Worse Prognosis and Promotes Hepatocellular Carcinoma Proliferation, Migration, Invasion and EMT By Modulating Wnt/β-Catenin Pathway
Authors: Cheng Xiaoye , Li Taiyuan , Shi Jun , Ye Shanping 
Published: September 2021

Abstract
Background: Stratifin (SFN) is closely related to the tumor progression. However, the role of SFN in hepatocellular carcinoma (HCC) is still unknown. The purpose of this study is to investigate the clinical value, biological role and regulatory mechanisms of SFN in HCC. Methods: Immunohistochemistry and RT-qPCR was used to explore SFN expression in HCC tissues and corresponding adjacent non-tumor tissues. The SFN expression profile data and clinical data of HCC patients were extracted from GEO, TCGA and Oncomine database. The univariate and multivariate analysis were used to investigate the prognosis value of the SFN gene in patients with HCC based on online database. The effects of SFN on HCC cell proliferation, migration, invasion was investigated by preforming CCK-8, colony formation, wound healing and Transwell assays. Xenograft nude mouse were used to observe the role of SFN on tumor growth. Western blotting was used to explore the genes associated with Epithelial mesenchymal transformation (EMT) and Wnt/β-catenin signaling. The luciferase reported assay was used to validate the activity of Wnt signal pathway.Results: In this study, we found SFN was upregulated in HCC cell lines and tissues. Clinically, SFN was positively associated with tumor size, degree of differentiation, TNM stage and vascular invasion. Survival analysis indicated that patients with high SFN levels had worse OS and DFS. SFN was an independent prognostic factor for HCC. Biologically, knockdown of SFN repressed tumor cell proliferation, migration, invasion, epithelial-to-mesenchymal transition (EMT) in vitro and tumor growth in vivo. Conversely, overexpression of SFN promoted these effects. Moreover, SFN promoted matrix metalloproteinases-2 (MMP-2) and MMP-9 expression. Mechanistically, SFN activated Wnt/β-catenin pathway by promoting GSK-3β phosphorylation, decreasing β-catenin phosphorylation, promoting β-catenin nuclear translocation, increasing c-Myc expression and inhibiting Axin2 expression. Furthermore, the TOP/FOP-Flash reporter assays indicated that SFN overexpression or SFN knockdown obviously up-regulated or down-regulated Wnt signaling activity. Conclusions: SFN indicates worse survival in HCC and promotes HCC growth, migration, invasion and EMT by activating Wnt/β-catenin pathway. Our results suggest SFN may become a prognostic factor and therapeutic target for patients with HCC in future.
Degradation of Phenol by Photocatalysis Using TiO2/Montmorillonite Composites Under UV Light
Authors: Li Huijuan , Yao Yeting , Yang Xiaoyan , Zhou Xusheng , Lei Ran , He Shufang 
Published: September 2021

Abstract
Composites of Titanium (IV) oxide combined with montmorillonite (MMT) with various TiO2/MMT were prepared for photocatalysis application. The prepared samples were characterized by X-ray diﬀraction (XRD), transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), and diﬀuse reﬂectance UV–visible spectroscopy. The main influential factors such as the TiO2/MMT dose, calcined temperature and pH value of the solution were studied. The main intermediates of phenol degradation were determined by High Performance Liquid Chromatography (HPLC). The results showed that the average size of TiO2 nanoparticles was decreased from 22.51 nm to 10.66 nm through the immobilization on MMT. The components in the interlayer domain were replaced by titanium pillars, and the pillaring reaction proceeded in the interlayer domain, and the basic skeleton of MMT was unchanged, and also TiO2 was dispersed on the surface of the MMT. When the initial concentration of phenol is 10 mg/L, the phenol solution pH is 6 and the UV light irradiation time is 240 min, the phenol degradation rate of 30%TiO2/MMT composite is 89.8%, which is better than MMT (11.5%) and pure TiO2 (58.8%). It shows that TiO2 loaded on MMT improves its photocatalytic activity. The phenol reaction process detected by HPLC showed that it had undergone through hydroquinone and benzoquinone, and finally converted into maleic acid and carbon dioxide and small molecules. The possible photocatalysis mechanism is presented.
The Relationship Between Organisational Culture, Leadership Supportive Climate and Hospital Employees’ Perception of Organisational Attractiveness and Level of Innovative Behaviour
Authors: Mutonyi Barbara Rebecca , Slåtten Terje , Lien Gudbrand 
Published: September 2021

Abstract
Background

Within the domain of health services research there has been little research focusing on how organisational culture is associated with leadership supportive climate and specifically whether and how organisational culture and leadership supportive climate either individually or mutually are related to employees’ perception of the attractiveness of the organisation and employees’ level of innovative behaviour. These knowledge gaps in previous research motivated the implementation of this study.
Methods

The conceptual model developed was tested on a sample (N = 1008) of hospital employees. Partial least squares structural equation modelling (PLS–SEM) was employed to test the conceptual models, using the SmartPLS 3 software. To test the mediator effect, a bootstrapping test was used, revealing whether the direct and indirect effects were statistically significant, and at the same time, when combining two tests, determining the type of mediator effect.
Results

The results can be summarized in four key findings: i) organisational culture was found to be positively directly related to leadership supportive climate (\(\beta\) = 0.87) and organisational attractiveness (\(\beta\) = 0.45), ii) leadership supportive climate was found to be positively directly related to both organisational attractiveness (\(\beta\) = 0.22) and employees’ individual innovative behaviour (\(\beta\) = 0.37), iii) the relationships between organisational culture, leadership supportive climate and employees’ innovative behaviour were both found to be mediated through organisational attractiveness, iv) leadership supportive climate was found to mediate the relationship between organisational culture and organisational attractiveness as well as the relationship between organisational culture and employees’ innovative behaviour.
Conclusions

Organisational culture and leadership supportive climate were highly correlated and imperative drivers to employees’ perception of organisational attractiveness and employees’ level of innovative behaviours. Managers of hospitals should consider organisational culture and leadership supportive climate as two organisational resources that are potentially manageable and controllable. Consequently, managers should actively invest in the two organisational resources. Such resource investment will lead to resource capitalisation manifested in both a growth in employees’ perception of organisational attractiveness as well as employees’ level of innovative behaviour.
∆9-Tetrahydrocannabinol Self-Administration Induces Cell-Type Specific Adaptations and cFOS Expression in the Nucleus Accumbens Core
Authors: Neuhofer Daniela , Garcia-Keller Constanza , Hohmeister Madeline , Seidling Kailyn , Beloate Lauren , Chioma Vivian , Spencer Sade , Kalivas Peter 
Published: September 2021

Abstract
Given that 30% of chronic cannabis users develop cannabis use disorder (CUD), it is critical to identify neuroadaptations that contribute to this disease. The nucleus accumbens core (NAcore) is important for drug seeking and ~ 90% of all NAcore neurons are divided into D1- and D2-medium spiny neurons (MSNs) that serve opposing roles in drug seeking. Drugs of abuse induce D1- and D2-MSN specific adaptations but whether ∆9-tetrahydrocannabinol (THC) use initiates similar neuroadaptations is unknown. D1- and D2-Cre transgenic rats were transfected with Cre-dependent reporters and trained to self-administer THC + cannabidiol (THC + CBD). After extinction training dendritic spine morphology, glutamate transmission, CB1R function and cFOS expression were quantified. We found that extinction from THC + CBD induced a loss of large spine heads in D1- but not D2-MSNs and a commensurate reduction in glutamate synaptic transmission. Also, CB1R function was impaired on glutamatergic synapses onto D1-MSNs and this was paralleled by an augmented capacity to potentiate glutamate transmission in D1-MSNs. CB1R function and glutamate synaptic transmission on D2-MSN synapses were unaffected by THC + CBD use. Using cFOS expression as an activity marker, we found that D1-MSNs activity remained unchanged after extinction from THC + CBD but significantly increased after 60 minutes cue-induced drug seeking. Surprisingly, the percentage of D2-MSNs expressing cFOS decreased after extinction from THC + CBD and this decrease was restored by drug cues. Thus, glutamatergic adaptations in D1-MSNs partially predict activity changes and pose modulating CB1R function that is down-regulated selectively at D1-MSN synapses as a potential treatment strategy for CUD.
Health Humanities Curriculum and Evaluation in Health Professions Education: A Scoping Review
Authors: Carr Sandra E , Noya Farah , Phillips Brid , Harris Anna , Scott Karen , Hooker Claire , Mavaddat Nahal , Ani-Amponsah Mary , Vuillermin Daniel M. , Reid Steve , MacLean Pamela Brett- 
Published: September 2021

Abstract
Background:The articulation of learning goals, processes and outcomes related to health humanities teaching currently lacks comparability of curricula and outcomes, and requires synthesis to provide a basis for developing a curriculum and evaluation framework for health humanities teaching and learning. This scoping review sought to answer, how, and why the health humanities are used in health professions education. It also sought to explore how health humanities curricula are evaluated and whether the programme evaluation aligns with the desired learning outcomes. Methods:A focused scoping review of qualitative and mixed-methods studies that included the influence of integrated health humanities curricula in pre-registration health professions education with programme evaluate of outcomes was completed. Studies of students not enrolled in a pre-registration course, with only ad-hoc health humanities learning experiences that were not assessed or evaluated were excluded. Four databases were searched (CINAHL), (ERIC), PubMed, and Medline.Results:The search over a 5 year period, identified 8,621 publications. Title and abstract screening, followed by full-text screening, resulted in 24 articles selected for inclusion. Learning outcomes, learning activities and evaluation data were extracted from each included publication. Discussion:Reported health humanities curricula focused on developing students’ capacity for perspective, reflexivity, self- reflection and person-centred approaches to communication. However the learning outcomes were not consistently described, identifying a limited capacity to compare health humanities curricula across programmes. A set of clearly stated generic capabilities or outcomes from learning in health humanities would be a helpful next step for benchmarking, clarification and comparison of evaluation strategy.
Perceptions of Orthopaedic Medicine Students and Their Supervisors About Practice-Based Learning: An Exploratory Qualitative study
Authors: Nsubuga Muhamed , Opoka Robert O , Galukande Moses , Munabi Ian G. , Mubuuke Aloysius G. , Kiguli Sarah 
Published: September 2021

Abstract
Background

Practice based learning is crucial in forming appropriate strategies for improving learning among the medical students that support the country’s understaffed health sector. Unsatisfactory learning consequently results into poor performance of students and poor quality of health care workforce in the long run. Exploring the perceptions about the current practice-based learning system and how to improve is thus vital. This study therefore set out to explore perceptions of Orthopaedic medicine students and their supervisors about practice-based learning at a tertiary training hospital.
Methods

This was an exploratory qualitative study that involved in-depth interviews among 10 orthopedic students during their rotation in the emergency ward of Mulago hospital and 6 of their supervisors. Interviews were audio recorded, transcribed and then imported into Atlas ti 8.3 for analysis. The data was coded and grouped into themes relating to perceptions of practice-based learning, general inductive analysis was used. The general inductive approach involved condensing the raw textual data into a brief and summary format. The summarized format was then analyzed to establish clear links between the perceptions of practice-based learning and the summary findings derived from the raw data.
Results

Perceptions explored in the in-depth interviews included: presence of too many students on the wards during the rotation, frequent stock-outs of supplies for learning and supervisors being overwhelmed caring for the large number of patients.
Conclusion

Barriers to satisfactory practice-based learning were overcrowding on the wards and insufficient training materials. In order to improve practice-based learning, adequate learning materials are required and the number of students enrolled need to be appropriate for the student – supervisors’ ratio.
Combinatorial Approach of Feature Generation for Traffic Event Detection using Social Media Data: CFGA
Authors: Chaturvedi Narayan , Toshniwal Durga , Parida Manoranjan 
Published: September 2021

Abstract
Confined use of road sensors limits the effectiveness of traffic disturbing event detection. In this context, Twitter is becoming popular among the people to share the events that affect the daily life. In this paper, a novel dictionary formation and a new feature generation approach is proposed to build an integrated machine learning framework to detect the traffic events. The proposed novel combinatorial feature generation approach (CFGA) uncovers appropriate associations among the keywords of tweet and extracts the correlated keyword sets to the data collected. Such keyword sets are denoted as set phrases . The set phrases may comprise of single or multiple words of a tweet. These set phrases may be used as keywords for event-related data collection or further analysis. The frequently occurring set phrases are identified using the notion of support, which signifies the percentage of tweets containing relevant keywords. Since the nature of different events may also vary; therefore, a hardcoded value for support thresh- old will not be beneficial. Hyper-parameter designated as support (!) is tuned for finding threshold value that is used to obtain the set phrases. This process sets up a database of frequently occurring set phrases that can signalize the traffic-related events using ML classifier. The results of the proposed approach suggest that if suitable support is chosen, proposed CFGA increases the accuracy of supervised classification models for extracting traffic information from Twitter data. The classification results obtained by using the proposed approach outperform

their existing counterparts in terms of precision, recall, and F-measure.
Short-Term High-Fat Diet Promotes Increased Lysine Crotonylation in Cerebral Cortex
Authors: Wang Junling , Zhang Jiajia , Wang Xiaolu , Xu Chaojin 
Published: September 2021

Abstract
Protein lysine crotonylation is a newly discovered protein post-translational modification (PTM), which has been associated with cellular metabolism, cell cycle, gene transcription, DNA damage response. However, its potential roles related to human central nervous system diseases remain largely unknown. In the present study, we observed a significant elevated lysine crotonylation in a screening of nine lysine acylations in cortex tissues of HFD-fed mice after short-term overfeeding. On the base of previous reports and molecular weight of proteins, we also speculate that actin, ERK2 or GAPDH and CDK1 might be modified by lysine crotonylation (KCr). Taken together, our findings highlight a potential role of protein lysine crotonylation in HFD-induced brain disorders and as possible therapeutic candidates in the future.