bluuebunny/crossref_metadata_2025_split huggingface dataset:
Dimerization underlies the aggregation propensity of intrinsically disordered coiled‐coil domain‐containing 124
Authors: Tuzlakoğlu Öztürk Merve , Güllülü Ömer 
Published: August 2021

AbstractCoiled‐coil domain‐containing 124 (CCDC124) is a recently discovered ribosome‐binding protein conserved in eukaryotes. CCDC124 has regulatory functions on the mediation of reversible ribosomal hibernation and translational recovery by direct attachment to large subunit ribosomal protein uL5, 25S rRNA backbone, and tRNA‐binding P/A‐site major groove. Moreover, it independently mediates cell division and cellular stress response by facilitating cytokinetic abscission and disulfide stress‐dependent transcriptional regulation, respectively. However, the structural characterization and intracellular physiological status of CCDC124 remain unknown. In this study, we employed advanced in silico protein modeling and characterization tools to generate a native‐like tertiary structure of CCDC124 and examine the disorder, low sequence complexity, and aggregation propensities, as well as high‐order dimeric/oligomeric states. Subsequently, dimerization of CCDC124 was investigated with co‐immunoprecipitation (CO‐IP) analysis, immunostaining, and a recent live‐cell protein–protein interaction method, bimolecular fluorescence complementation (BiFC). Results revealed CCDC124 as a highly disordered protein consisting of low complexity regions at the N‐terminus and an aggregation sequence (151‐IAVLSV‐156) located in the middle region. Molecular docking and post‐docking binding free energy analyses highlighted a potential involvement of V153 residue on the generation of high‐order dimeric/oligomeric structures. Co‐IP, immunostaining, and BiFC analyses were used to further confirm the dimeric state of CCDC124 predominantly localized at the cytoplasm. In conclusion, our findings revealed in silico structural characterization and in vivo subcellular physiological state of CCDC124, suggesting low‐complexity regions located at the N‐terminus of disordered CCDC124 may regulate the formation of aggregates or high‐order dimeric/oligomeric states.
A Phase 1 first‐in‐human study of the safety, tolerability, and pharmacokinetics of the ROBO2 fusion protein PF‐06730512 in healthy participants
Authors: Lim Chay Ngee , Kantaridis Constantino , Huyghe Isabelle , Gorman Donal , Berasi Stephen , Sonnenberg Gabriele E. 
Published: August 2021

AbstractProteinuria associated with podocyte effacement is a hallmark of focal segmental glomerulosclerosis (FSGS). Preclinical studies implicated ROBO2/SLIT2 signaling in the regulation of podocyte adhesion, and inhibition of this pathway is a novel target to slow FSGS disease progression. This first‐in‐human dose‐escalation study evaluated the safety, tolerability, pharmacokinetics, and immunogenicity of PF‐06730512, an Fc fusion protein that targets the ROBO2/SLIT2 pathway, in healthy adults. In this Phase 1, double‐blind, sponsor‐open study, single ascending dose (SAD) cohorts were randomized to receive up to 1000 mg or placebo intravenously (IV); multiple ascending dose (MAD) cohorts were randomized to receive up to 400 mg subcutaneous (SC) doses, 1000 mg IV dose, or matching placebo. Safety evaluations were performed up to 71 (SAD) and 113 (MAD) days after dosing; blood samples were collected to measure serum PF‐06730512 concentrations and antidrug antibodies (ADA) to PF‐06730512. Seventy‐nine participants (SAD, 47; MAD, 32) were enrolled. There were 108 mild (SAD, 46; MAD, 62) and 21 moderate (SAD, 13; MAD, 8) treatment‐emergent adverse events (TEAEs); no deaths, treatment‐related serious AEs, severe TEAEs, or infusion reactions were reported. PF‐06730512 exposure generally increased in an approximately dose‐proportional manner; mean t1/2 ranged from 12–15 days across 50–1000 mg doses. Immunogenicity incidence was low (SAD, 0 ADA+; MAD, 2 ADA+). In conclusion, single IV doses of PF‐06730512 up to 1000 mg and multiple IV and SC dosing up to 1000 and 400 mg, respectively, were safe and well tolerated in healthy participants. Further trials in patients with FSGS are warranted.Clinical trial registration: Clinicaltrials.gov: NCT03146065.
A case of granulomatosis with polyangiitis mimicking lung malignancy
Authors: Han Michael , Pan Corinna 
Published: August 2021

AbstractPatients with granulomatosis with polyangiitis (GPA) may present with varied manifestations including pulmonary masses and nodules. We report the case of a 45‐year‐old man presenting with cough, haemoptysis and weight loss in the context of a 20 pack‐year past smoking history. Computed tomography (CT) of the chest and positron emission tomography/CT scan demonstrated two right upper lobe masses, bilateral lung nodules and mediastinal lymphadenopathy, with increased fluorodeoxyglucose avidity. Endobronchial and CT‐guided lung biopsy demonstrated granulomatous inflammation and elevated c‐ANCA/PR3 confirmed the diagnosis of GPA. The patient received induction therapy with methylprednisolone and rituximab with good clinical response. Our case highlights the importance of considering a wide range of differentials in patients with lung masses/nodules, including autoimmune pathologies.
Brightening of Dark States in CsPbBr3 Quantum Dots Caused by Light‐Induced Magnetism
Authors: Lohmann Sven‐Hendrik , Cai Tong , Morrow Darien J. , Chen Ou , Ma Xuedan 
Published: August 2021

AbstractLead halide perovskite quantum dots (QDs) have shown great potential for optoelectronic and quantum photonic applications. Although controversy remains about the electronic fine structures of bulk perovskites due to the strong spin‐orbit coupling affecting the conduction bands, compelling evidence indicates that the ground states of perovskite QDs remain dark, limiting their applications in optoelectronic devices. Here, it is demonstrated that photoexcitation can induce large intrinsic magnetic fields in Mn‐doped CsPbBr3 perovskite QDs. Equivalent to applying an external magnetic field, the light‐induced field causes giant Zeeman splitting to the bright triplet states and brightens the dark singlet ground state, thus effectively rendering a partially bright ground state in the doped QDs. These findings here may contribute to the understanding of the electronic fine structures in perovskite QDs and demonstrate a potential approach for creating semiconductor nanostructures that can serve as bright light sources.
Real‐Time Biomonitoring Device Based on 2D Black Phosphorus and Polyaniline Nanocomposite Flexible Supercapacitors
Authors: Vaghasiya Jayraj V. , Křípalová Kristýna , Hermanová Soňa , Mayorga‐Martinez Carmen C. , Pumera Martin 
Published: August 2021

AbstractFlexible energy storage devices are becoming significantly important to power wearable and portable devices that monitor physiological parameters for many biomedical applications. Many hybrid nanomaterials based on 2D materials are used in order to improve the performance of flexible energy storage devices. Here, a hybrid nanocomposite is synthesized through in situ polymerization of aniline in the presence of black phosphorus (BP) nanoflakes. This nanocomposite, polyaniline (PANI)@BP, is employed to fabricate flexible supercapacitor (FSC) electrodes. PANI@BP FSCs can provide a power source for biometric devices. The generated signal can be transmitted to a smartphone in real time via wireless communication. Such a compact and lightweight integrated device has been used to track a human heart beat while powered by PANI@BP FSC. These findings are providing a promising example of a flexible energy storage device that can be integrated with different real‐time health monitoring devices.
Guar Gum Nanocomposites: Green Corrosion Inhibitors of Iron‐Gall Ink on Paper Artworks
Authors: Afsharpour Maryam , Gilani Sorror 
Published: August 2021

AbstractIron‐Gall ink corrosion in paper artworks has always been a major challenge for restorers. In this study, a novel green nanocomposite based on Guar gum that can be used as a protective coating to prevent corrosion induce by oxidation and the acid hydrolysis process is introduced. For this purpose, Guar gum/calcium hydroxide/Klucel nanocomposite is used as an effective coating to stabilize the iron on paper and slow down the deterioration process. Guar gum is used as a chelating agent for Fe(II), Ca(OH)2 nanoparticles can deacidify the paper, and Klucel contributes to better dispersion of the nanoparticles and increase the mechanical strength of the paper and also can contribute to stabilizing Fe ions. This one‐step process for consolidation, deacidification, and antioxidation is a good way to decrease the physical damage, especially to sensitive papers, and saves time and materials too. To monitor the treatment effects over time, accelerated aging is also studied. Results show the good protective effects of nanocomposite coatings on the paper which retains its strength even after thermal aging due to a decrease in the rate of oxidation and hydrolysis reactions.
Fermentation of whey and its permeate using a genetically modified strain of Escherichia coli K12 MG1655 to produce 2,3‐butanediol
Authors: Fernández‐Gutiérrez David , Veillette Marc , Ávalos Ramirez Antonio , Giroir‐Fendler Anne , Faucheux Nathalie , Heitz Michèle 
Published: August 2021

AbstractWhey is generated during cheese manufacturing, and permeate whey is obtained after whey deproteinization. Both effluents contain lactose, which can cause environmental issues if they are released into the environment. The aim of the present study was the valorization of lactose contained in whey and permeate whey via fermentation into 2,3‐butanediol (2,3‐BD) with genetically modified strain of Escherichia coli K12 MG1655. Both effluents were first fermented at various dilution ratios with the culture medium M9 (50:50, 75:25, and 100:0, v/v). The fermentation of undiluted effluents produced the highest 2,3‐BD yield (0.43 g/g lactose) at 72 h. Afterwards, the effects of initial pH, inoculum size and agitation rate on 2,3‐BD yield in flask fermentation of undiluted effluents were studied. The agitation rates of 50 and 200 rpm resulted in lower 2,3‐BD yields compared with 100 rpm (67% lower). The effect of aeration (2.5 vvm) on 2,3‐BD yield was tested in a 2 L bioreactor, where a 2,3‐BD yield of 0.40 g/g lactose was obtained after 24 h. In the present study, it was demonstrated that both whey and permeate whey can be used to produce 2,3‐BD, reaching near 80% of the theoretical yield after 24 h of fermentation.
Mn2+ substitution within the {V14As8} polyoxovanadate archetype results in {Mn2V12As8} shells with trans‐positioned heterometal positions
Authors: Rasmussen Maren , Näther Christian , Kögerler Paul , Bensch Wolfgang 
Published: August 2021

AbstractSolvothermal exploration of the As, V, Mn reaction system in the presence of aqueous diethylenetriamine (=dien) solution led to the discovery of [MnII4VIV12AsIII8O40(dien)4(H2O)] ⋅ 3.5H2O, containing the first arsenato‐polyoxovanadate featuring a {Mn2V12As8O40} cluster shell. In comparison to its parental {V14As8O42} archetype, two vanadyl (VO2+) units at opposite ends of the polyoxovanadate shell here are substituted by Mn2+ centers. In the solid state, these {Mn2V12As8O40} units are joined into rods by sharing common Mn−O−V linkages. Expansion of the cluster shell by two further Mn2+ sites via Mn−O=V bridges generates a {Mn4V12As8O40} moiety. Three Mn2+ cations are joined by monodentate‐coordinating dien ligands, which link the rods into layers. The dien ligands here coexist in three different coordination modes: monodentate, bidentate and tridentate, which is rarely observed. Magnetic susceptibility measurements show the expected significant antiferromagnetic coupling interactions between all spin centers present in the title compound, namely the isotropic spin‐5/2 Mn2+ centers and the isotropic spin‐1/2 vanadyl groups, in line with most other heterometal‐substituted arsenato‐polyoxovanadate(IV) clusters.
Numerical semigroups bounded by the translation of a plane monoid
Authors: Moreno-Frías M. A. , Rosales J. C. 
Published: August 2021

AbstractLet $$\mathbb {N}$$
N
be the set of nonnegative integer numbers. A plane monoid is a submonoid of $$(\mathbb {N}^2,+)$$

(

N

2

,
+
)

. Let M be a plane monoid and $$p,q\in \mathbb {N}$$

p
,
q
∈
N

. We will say that an integer number n is M(p, q)-bounded if there is $$(a,b)\in M$$

(
a
,
b
)
∈
M

such that $$a+p\le n \le b-q$$

a
+
p
≤
n
≤
b
-
q

. We will denote by $${\mathrm A}(M(p,q))=\{n\in \mathbb {N}\mid n \text { is } M(p,q)\text {-bounded}\}.$$

A
(
M
(
p
,
q
)
)
=
{
n
∈
N
∣
n

is

M
(
p
,
q
)
-bounded
}
.

An $$\mathcal {A}(p,q)$$

A
(
p
,
q
)

-semigroup is a numerical semigroup S such that $$S= {\mathrm A}(M(p,q))\cup \{0\}$$

S
=
A
(
M
(
p
,
q
)
)
∪
{
0
}

for some plane monoid M. In this work we will study these kinds of numerical semigroups.
Intensivbettenbedarf für COVID‑19 im Herbst/Winter 2021
Authors: Schuppert Andreas , Weber-Carstens Steffen , Karagiannidis Christian 
Published: August 2021

Zusammenfassung
Hintergrund
Auch im Herbst 2021 droht trotz der steigenden Impfquoten gegen SARS-CoV-2 aufgrund saisonaler Veränderungen und der damit verbundenen erhöhten Infektionsraten eine erneute Infektionswelle mit einhergehender erneuter möglicher starker Belastung der Intensivmedizin. Im Folgenden sind verschiedene Szenarien mithilfe mathematischer Modelle simuliert, die unter der Annahme bestimmter Voraussetzungen eine Einschätzung hinsichtlich der Auslastung der Intensivbettenkapazitäten im Herbst in bestimmten Grenzen ermöglichen.

Methodik
Die Simulation der Szenarien verwendet ein stationäres Modell, ergänzt um den Effekt der Impfungen. Das altersgruppenspezifische Risikoprofil für einen intensivpflichtigen Krankheitsverlauf wird anhand von Einweisungsdaten der 3. Welle auf Intensivstationen in Sentinel-Kliniken, den lokalen DIVI-Register-Belegungsdaten sowie den entsprechenden lokalen Inzidenzen durch lineare Regression mit Zeitversatz berechnet. Wir simulieren hierbei Impfquoten von 15 % für die U18-Kohorte, 70 % für die 15- bis 34-jährige Kohorte, 75/80/85 % für die Kohorte von 35 bis 59 Jahren sowie 85/90/95 % für die Ü60-Kohorte. Die Simulationen berücksichtigen, dass eine Impfung zu 100 % vor einem intensivpflichtigen Krankheitsverlauf schützt. Für den Schutz vor Infektion der Geimpften wurden die Simulationen zum einen für das Szenario Impfschutz für 70 % der Geimpften und zum anderen für das Szenario Impfschutz für 85 % der Geimpften dargestellt.

Ergebnisse
Die Intensivbettenauslastung verläuft proportional zur Inzidenz. Der Faktor für dieses Verhältnis (Proportionalitätsfaktor) ist höher als in der 2. und 3. Welle, sodass vergleichbare Intensivbettenbelegungen erst bei höherer Inzidenz erreicht werden. Eine 10 %ige Steigerung der Impfquoten der Ü35 auf 85 % und der Ü60 auf 95 % führt zu einer erheblich verringerten Intensivbettenbelegung.

Diskussion
Es besteht auch in den kommenden Monaten eine enge und lineare Beziehung zwischen der SARS-CoV-2-Inzidenz und der Intensivbettenbelegung. Bereits ab Inzidenzen von 200/100.000 ist wieder eine erhebliche Belastung der Intensivstationen mit mehr als 3000 COVID-19-Patienten zu erwarten, sofern die Impfquote nicht noch deutlich gesteigert wird. Wenige Prozentpunkte in der Impfquote haben eine erhebliche Auswirkung auf die potenzielle Intensivbelegung im Herbst, sodass Bemühungen um die Steigerung der Impfakzeptanz in den kommenden Wochen im Vordergrund stehen sollten. Für die Intensivmedizin ist die Impfquote der über 35-Jährigen von entscheidender Bedeutung.
Women’s experiences of the injury, recovery and desire for rehabilitation after a second-degree vaginal tear—a qualitative study
Authors: Daremark Clara , Andréasson Lina , Gutke Annelie , Fagevik Olsén Monika 
Published: August 2021

Abstract
Introduction and hyposthesis
Eighty-five percent of all vaginal deliveries cause some form of obstetric tear injury. To our knowledge, there are no studies exploring experiences after second-degree tear. Therefore, our study aimed to investigate the experiences of a second-degree vaginal tear regarding aspects of the recovery and need for healthcare and rehabilitation.

Methods
Individual semi-structured interviews were performed and analysed with a qualitative, inductive descriptive approach.

Results
A group of 18 women with a second-degree vaginal tear after delivery were included. Four main categories with associated subcategories were found: (1) feeling uncertainty, with subcategories: not knowing what is normal, concern, confusion and uncertainty regarding pelvic floor muscle training; (2) feeling of security, with subcategories: I have no/I can handle the symptoms, trust in the healthcare system and I have sufficient knowledge; (3) not prioritizing myself, with the subcategories: I cannot find time and others have bigger problems; (4) lack of trust in healthcare providers, with the subcategories: feeling forgotten, not being taken seriously, distrust of the competence of the healthcare providers and resignation.

Conclusion
Women who suffer from a second-degree vaginal tear after pregnancy can feel safe when needs are met but uncertainty is also common when available healthcare and information are perceived as insufficient. The women also feel uncertainty about what is normal after the tear and how to perform pelvic floor exercises.

Trial registration
This trial was registered in “FoU in Sweden” (Research and Development in Sweden). Registration number: 214591.
The role of cochlear implant positioning on MR imaging quality: a preclinical in vivo study with a novel implant magnet system
Authors: Canzi Pietro , Magnetto Marianna , Simoncelli Anna , Manfrin Marco , Aprile Federico , Lafe Elvis , Carlotto Elena , Avato Irene , Scribante Andrea , Preda Lorenzo , Benazzo Marco 
Published: August 2021

Abstract
Purposes
To investigate the effects for Ultra 3D cochlear implant (CI) positioning on MR imaging quality, looking at a comprehensive description of intracranial structures in cases of unilateral and bilateral CI placement.

Methods
Four CI angular positions (90°, 120°, 135° and 160°) at 9 cm distance from the outer-ear canal were explored. The 1.5 T MRI assessment included our institutional protocol for the investigation of brain pathologies without gadolinium application. Three investigators (two experienced neuroradiologists and one experienced otoneurosurgeon) independently evaluated the MR findings. A 4-point scale was adopted to describe 14 intracranial structures and to determine which CI positioning allowed the best image quality score and how bilateral CI placement modified MRI scan visibility.

Results
A high positive correlation was found between the three blinded observers. Structures situated contralateral from the CI showed high-quality values in all four placements. Structures situated ipsilaterally provided results suitable for diagnostic purposes for at least one position. At 90°, artifacts mainly involved brain structures located cranially and anteriorly (e.g., temporal lobe); on the contrary, at 160°, artifacts mostly influenced the posterior fossa structures (e.g., occipital lobe). For the bilateral CI condition, MR imaging examination revealed additional artifacts involving all structures located close to either CI, where there was a signal void/distortion area.

Conclusions
Suitable unilateral CI positioning can allow the visualization of intracranial structures with sufficient visibility for diagnostic purposes. Bilateral CI positioning significantly deteriorates the anatomical visibility. CI positioning might play a crucial role for patients who need post-operative MRI surveillance.
Role of ascorbic acid in the regulation of epigenetic processes induced by Porphyromonas gingivalis in endothelial-committed oral stem cells
Authors: Pizzicannella Jacopo , Marconi Guya Diletta , Guarnieri Simone , Fonticoli Luigia , Della Rocca Ylenia , Konstantinidou Fani , Rajan Thangavelu Soundara , Gatta Valentina , Trubiani Oriana , Diomede Francesca 
Published: August 2021

AbstractPeriodontitis is a common inflammatory disease that affects the teeth-supporting tissue and causes bone and tooth loss. Moreover, in a worldwide population, periodontal disease is often associated with cardiovascular diseases. Emerging studies have reported that one of the major pathogens related to periodontitis is Porphyromonas gingivalis (P. gingivalis), which triggers the inflammatory intracellular cascade. Here, we hypothesized a possible protective effect of ascorbic acid (AA) in the restoration of the physiological molecular pathway after exposure to lipopolysaccharide derived from P. gingivalis (LPS-G). In particular, human gingiva-derived mesenchymal stem cells (hGMSCs) and endothelial-differentiated hGMSCs (e-hGMSCs) exposed to LPS-G showed upregulation of p300 and downregulation of DNA methyltransferase 1 (DNMT1), proteins associated with DNA methylation and histone acetylation. The co-treatment of AA and LPS-G showed a physiological expression of p300 and DNMT1 in hGMSCs and e-hGMSCs. Moreover, the inflammatory process triggered by LPS-G was demonstrated by evaluation of reactive oxygen species (ROS) and their intracellular localization. AA exposure re-established the physiological ROS levels. Despite the limitations of in vitro study, these findings collectively expand our knowledge regarding the molecular pathways involved in periodontal disease, and suggest the involvement of epigenetic modifications in the development of periodontitis.
Relationship between type of skin lesions and nailfold capillaroscopy pattern in mixed connective tissue disease
Authors: Felis-Giemza Anna , Ornowska Sylwia , Haładyj Ewa , Czuszyńska Zenobia , Olesińska Marzena 
Published: August 2021

Abstract
Introduction
Mixed connective tissue disease (MCTD) is a rare disease with clinical picture consisted of multiple organ manifestations, including skin changes resembling systemic lupus erythematosus (SLE), systemic sclerosis (SSc), or dermatomyositis (DM). On the background of these manifestations are microvascular changes — alteration of endothelial function and impairment of endothelial progenitor cell. Nailfold capillaroscopy (NFC) is a simple, non-invasive technique for investigating microvascular involvement in rheumatic diseases.

Objectives
To describe the relationship between type of skin lesions and NFC pattern in MCTD patients.

Methods
We analyzed the clinical picture and NFC patterns in 79 patients with MCTD. The NFC changes were classified into Normal, “Early,” “Active,” and “Late” scleroderma-like patterns (SD-like pattern) based on Cutolo classification.
In all patients, subjective and physical examinations were carried out, specifically the occurrence of skin lesions in the course of MCTD was assessed (systemic sclerosis-like (Ssc-like), systemic lupus erythematosus-like (SLE-like), dermatomysitis-like (DM-like)).

Results
Skin changes were present in 64 (81%) patients, involving 43 (54%) SLE-like, 48 (61%) SSc-like, and 4 (5.1%) DM-like. NFC changes were observed in a total of 55 (69.6 %) patients with predominance of the “Early” pattern — 41 (51.9 %) patients. According to skin change phenotypes, NFC changes were observed in 31 (72%) patients with SLE-like and in 32 (66.7%) patients with SSc-like skin phenotypes. The “early” pattern predominated in both group.

Conclusions
We did not find any correlation between NFC pattern and the type skin changes.

Key Points• The study did not show a correlation between the presence and absence of skin lesions and NFC pattern.• Scleroderma-like patterns were found in over 60% of patients with mixed connective tissue disease.• The “early” pattern is dominant regardless of the occurrence or absence of skin lesions in patients with MCTD.• Skin lesions, regardless of their type (SLE or SSc), do not correlate with type of lesion found in the NFC examination.