bluuebunny/crossref_metadata_2025_split huggingface dataset:
Transfer of hatched blastocyst can result in skewed sex ratio: a retrospective cohort study
Authors: Zhu Jinliang , Lian Ying , Zhuang Xinjie , Lin Shengli , Zheng Xiaoying , Li Junsheng , Liu Ping , Li Rong 
Published: November 2021

Abstract
Background
The correlation between blastocyst quality and birthweight, neonatal outcomes is still controversial. There is a significantly higher male: female ratio among good quality blastocysts (advanced trophoderm morphology) but in the expansion degree, the significance for sex ratio is unclear.
Methods
A total of 617 and 6803 live singleton births resulting from the transfer of fresh and frozen-thawed single blastocysts in the Reproductive Medicine Center of Peking University Third Hospital from 2009 to 2020 were included. Live singleton births from fresh and frozen-thawed single blastocyst transfer were stratified by inner cell mass/trophoderm morphology and degree of blastocoel expansion. Multivariate linear regression was used to analyze the correlation between expansion, inner cell mass/trophoderm morphology, and birthweight, Z score, gestational weeks. Logistic regression was used to analyze the relationship between expansion, ICM/TE morphology and sex, neonatal outcomes.
Results
There was no significant correlation between birthweight, neonatal outcomes and blastocyst quality in fresh and frozen-thawed single blastocyst transfer cycles. However, the proportion of male infants in the hatched blastocyst (stage-6) group (67.9% vs. 54.2%; p < 0.001) [OR: 1.76 95% CI (1.34–2.32)] and hatching blastocyst (stage-5) group (61.7% vs. 54.2%; p = 0.001) [OR: 1.36 95 C.I (1.14ཞ1.62)] was significantly higher than that in the expanded blastocyst (stage-4) group.
Conclusions
The transfer of poor-quality blastocysts is unlikely to affect birthweight and neonatal health; however, transfer of stage-6 blastocysts can result in extremely skewed sex ratio.
New Approaches for Micropropagation and Cryopreservation of Agave Peacockii, An Endangered Species
Authors: Delgado-Aceves Lourdes , Portillo Liberato , Folgado Raquel , Romo-Paz Felipe de Jesús , Gonzalez-Arnao Maria Teresa 
Published: November 2021

Abstract
More than 50% of Agave species are endemic to Mexico. Among them, Agave peacockii is listed within the list of threatened species that require special protection. In this work, we aimed at developing new supplementary strategies to achieve micropropagation and perform cryopreservation of in vitro-grown shoot-tips of A. peacockii. For multiplication, the addition of two cytokinins, 6-benzylaminopurine (26.6 μM) and kinetin (27.84 μM) to MS semisolid medium significantly favoured the morphogenetic response and produced the highest shoot generation (87.00±17.18) after 60 d of culture. This interaction was more effective than using the same growth regulators separately. Propagated and rooted plantlets were successfully acclimated with 100% survival and a normal morphological development during greenhouse performance. For cryopreservation, an optimized protocol following droplet-vitrification approach allowed obtaining 98% and 96% regrowth before and after cryopreservation, respectively. Shoot-tips were excised of in vitro-propagated plants, subjected to preculture on MS semisolid medium with 0.3 M sucrose for 1d, loaded in solution with 0.4 M sucrose and 1.6 M glycerol for 20 min, exposed to vitrification solution PVS2 for 15 min, and then, immersed in liquid nitrogen in droplets of PVS2 placed on aluminium foil strips. The vegetative growth of cryo-derived plants and of the in vitro propagated plants was compared under greenhouse culture conditions. No significant differences were detected in most assessed characteristics after 120 d of acclimatization. The results presented here constitute new viable biotechnological approaches for the in vitro propagation and long-term conservation of endangered Agave germplasm.
Research on Robustness of Geometric Algebra Based Adaptive Filtering Algorithms In Non-Gaussian Environment
Authors: Wang Rui , Wang Yi , Li Yanping , Cao Wenming 
Published: November 2021

Abstract
In this paper, two new geometric algebra (GA) based adaptive filtering algorithms in non-Gaussian environment are proposed, which are deduced from the robust algorithms based on the minimum error entropy (MEE) criterion and the joint criterion of the MEE and the mean square error (MSE) with the help of GA theory. Some experiments validate the effectiveness and superiority of the GA-MEE and GA-MSEMEE algorithms in α-stable noise environment. At the same time, the GA-MSEMEE algorithm has faster convergence speed compared with the GA-MEE.
Positioning And Power Optimization For UAV-Assisted Networks In The Presence of Eavesdroppers: A Multi-Armed Bandit Approach
Authors: Cabezas Xavier Alejandro Flores , Osorio Diana Pamela Moya , Latva-aho Matti 
Published: November 2021

Abstract
Unmanned Aerial Vehicles (UAVs) are becoming increasingly attractive for the ambitious expectations for 5G and beyond networks due to their several benefits. Indeed, UAV-assisted communications introduce a new range of challenges and opportunities regarding the security of these networks. Thus, in this paper we explore the opportunities that UAVs can provide for physical layer security solutions. Particularly, we analize the secrecy performance of a ground wireless communication network assisted by two friendly UAV jammers in the presence of an eavesdropper. To tackle the secrecy performance of this system, we introduce a new area-based metric, the weighted secrecy coverage, that measures the improvement on the secrecy performance of a system over a certain physical area given by the introduction of friendly jamming. Herein, the optimal 3D positioning of the UAVs and the power allocation is addressed in order to maximize the WSC. For that purpose, we provide a Reinforcement Learning-based solution by modeling the positioning problem as a Multi-Armed Bandit problem over three positioning variables for the UAVs: angle, height and orbit radius. Our results show that there is a trade-off between expediency of the positioning of the UAVs to positions of better secrecy outcome and energy expenditure, and that the proposed algorithm efficiently converges into a stable state.
Transient dendritic cell activation diversifies the T cell response to acute infection
Authors: Spitzer Matthew , Hiam-Galvez Kamir , DeBarge Rachel , Lareau Caleb , Cho Nam Woo , Yee Jacqueline , Okholm Trine Line 
Published: November 2021

Abstract
The precise timing of T cell priming during infection remains unclear. Here, we mapped the cellular dynamics of all immune lineages during acute infection with Listeria
monocytogenes (Lm). We identified highly transient activation of conventional type 1 dendritic cells (cDC1s) two days post-infection that functions as a critical time window for priming effector CD8 T cells. Regulation of this transient state was mediated by cDC1-extrinsic IFNγ provided by lymphocytes. Furthermore, antigen-specific T cells that are primed by cDC1s even shortly after this window of peak activation acquire only memory T cell fates. This temporal regulation of fate is recapitulated by cDC1s ex vivo, demonstrating that shifts in activation state of a single antigen presenting cell subset over time regulates CD8 T cell fates. These results uncover a novel mechanism for temporal regulation of CD8 T cell differentiation during a dynamic immune response to acute infection.
WITHDRAWN: Data Science: Trends, Perspectives, and Prospects
Authors: Borjigin Chaolemen , Zhang Chen 
Published: November 2021

Abstract
Data Science is one of today’s most rapidly growing academic fields and has significant implications for all conventional scientific studies. However, most of the relevant studies so far have been limited to one or several facets of Data Science from a specific application domain perspective and fail to discuss its theoretical framework. Data Science is a novel science in that its research goals, perspectives, and body of knowledge is distinct from other sciences. The core theories of Data Science are the DIKW pyramid, data-intensive scientific discovery, data science lifecycle, data wrangling or munging, big data analytics, data management and governance, data products development, and big data visualization. Six main trends characterize the recent theoretical studies on Data Science: growing significance of DataOps, the rise of citizen data scientists, enabling augmented data science, diversity of domain-specific data science, and implementing data stories as data products. The further development of Data Science should prioritize four ways to turning challenges into opportunities: accelerating theoretical studies of data science, the trade-off between explainability and performance, achieving data ethics, privacy and trust, and aligning academic curricula to industrial needs.
Study of lncRNA TUG1/miR-320 in EPC Transplantation to Improve Lower Limb Ischemia and Promote Angiogenesis in Mice
Authors: Qian Wei , Yan Qiong , Jiang Xinmiao , Nie Jungang , He Jiaqi , zhou Fangbin , Cao Xiaoyan , Kang Ting 
Published: November 2021

Abstract
To The aim of the current study was to investigate the changes in lncRNA TUG1/miR-320 and related proteins with ischaemic time in an ischemia model. A nude mouse model of lower limb ischemia was established by ligating the femoral artery, and laser Doppler measurements were used to demonstrate the successful establishment of the ischemia model. The cells were extracted from the bone marrow of nude mice, and the proliferation, migration and vascular-forming ability of the cells were analysed. When transplanted into ischemia model mice, blood flow measurements indicated that EPCs can speed up blood flow recovery. The results of HE staining indicated an improvement in inflammatory damage, and immunohistochemistry revealed an increase in capillaries. RT-PCR and Western blot experiments showed that the improvement of ischemia was related to an increase in lncRNA TUG1 and a decrease in miR-320 and that the expression of the related downstream proteins STAT3, VEGFR-2, Wnt-5a and β-catenin increased gradually. These changes promoted an increase in capillaries, the recovery of blood flow, and the improvement of muscle damage. Therefore, EPC transplantation can improve the inflammatory response of lower limb muscles by increasing the expression of lncRNA TUG1 and thereby accelerate the recovery of ischaemic limbs.
Thermal Stability of Silicon-Doped Tetrahedral Amorphous Diamond-Like Carbon Coatings And Improvement of Tribological Properties Through High- Temperature Annealing
Authors: Jang Young-Jun , Kim Jae-Il , Kim Won-seok , Jang Ji-Woong , Kim Dohyun , Kim Jongkuk 
Published: November 2021

Abstract
We report the structure, mechanical properties, thermal stability, and durability of Si-doped tetrahedral amorphous carbon (Si-ta-C) coatings fabricated using simultaneous filtered cathodic vacuum arc deposition and direct current unbalanced magnetron sputtering. Si doping of 1.25–6.04 at.% was achieved by increasing the unbalanced magnetron sputtering power from 25 to 175 W. Si doping provided functionality to the coating, such as heat resistance, while retaining the high hardness of ta-C coatings. The Si-ta-C coatings were stable up to 600 °C regardless of the Si content, while the coating containing 3.85 at.% Si was stable up to 700 °C. The friction behavior and mechanical properties were dependent on the coating film before and after annealing at 100–200 °C; however, annealing at 300–400 °C decreased disk wear and increased counterpart wear due to an increase in film hardness on account of an endothermic reaction that increased the number of Si–C bonds. This indicates that the basic hardness characteristics of the ta-C coating and the high-temperature structural change of the Si-ta-C coating are important for ensuring high-temperature durability. These characteristics were verified through the low coefficient of friction and wear rate of the 1.25 at.% Si-ta-C coating after annealing at 500 °C.
Total Laparoscopic Resection by Retroperitoneal Approach Using Virtual Navigation: Two Case Reports of Primary Retroperitoneal Schwannoma
Authors: Sato Yuta , matsuhashi Nobuhisa , Sugie Yuto , Kuno Masashi , Kiyama Shigeru , Tanaka Yoshihiro , Okumura Naoki , Takahashi Takao , Saiki Takuya , Yoshida Kazuhiro 
Published: November 2021

Abstract
Background: We report two rare cases of retroperitoneal schwannoma completely resected by laparoscopic retroperitoneal approach aided by virtual navigation.Case presentation: These two case reports are of a 60‐year‐old man and a 40‐year‐old man with asymptomatic retroperitoneal schwannoma. In both cases, the tumors were located in the right renal hilum and were close to the duodenum, right ureter, and inferior vena cava. Simulation using three-dimensional images was performed before surgery, and a retroperitoneal approach was performed to secure a wide surgical field. During the operation, we confirmed the location of the main feeder and the relationship between the tumor and organs with those shown on the three-dimensional images and performed total laparoscopic resection.Conclusion: The retroperitoneal approach provides operative safety. Preoperative simulation and intraoperative navigation with three-dimensional images are useful methods to enhance the surgeon’s understanding of a patient’s specific anatomy and are especially effective when resecting a retroperitoneal tumor that is located in an anatomically deep and complex location.
Association of Appetite and Related Hormones (Leptin, Ghrelin) with Resting Metabolic Rate of Overweight/ Obese Women: A Case-Control Study
Authors: Hajishizari Sara , Imani Hossein , Mehranfar Sanaz , Yekaninejad Mir Saeed , Mirzaei khadijeh 
Published: November 2021

Abstract
Objective: Low resting metabolic rate (RMR) as a risk factor for weight gain and obesity can be influenced by many factors. Researchers have pointed out the role of appetite and related hormones in obesity and energy intake. The aim of this study was to investigate the relationship between appetite and related hormones in the two groups with normal and impaired resting metabolic rate in overweight or obese Iranian women.Methods: In this case-control study, 42 healthy female volunteers aged 18-50 years (21 cases,and 21 controls) participated and were evaluated for RMR by indirect calorimetry. Body composition was determined by a body composition analyzer. The Filint questionnaire was used to assess appetite. We also used ELISA kits to assess appetite-related hormones. Finally, the data were analyzed using SPSS software version 25.Results: The results of the study showed that there is a negative relationship between ghrelin hormone level (p-value = 0.024) and RMR, and there is a positive relationship between insulin hormone level (p-value = 0.001) and RMR. Also, result of the appetite questionnaire showed that in general, both appetite (p-value = 0.044) and hunger index (p-value = 0.043) have a positive relationship with RMR. There was no significant relationship between leptin levels and RMR. Conclusion: As the results of this study show, it seems that appetite and related hormones have a possible role in promoting RMR.
Virus-Host Interactions Reveal Potential Roles Towards Phosphorus Cycling in South Atlantic Ocean Euphotic Zones
Authors: Lunga Percy Mutseka , Bezuidt Oliver K.I , Hirai Miho , Takaki Yoshihiro , Yokokawa Taichi , Nunoura Takuro , Murrell J. Colin , Makhalanyane Thulani 
Published: November 2021

Abstract
BackgroundDue to their role as obligate parasites of marine microorganisms, viruses are primary mediators of marine biogeochemical cycles. Recent studies have provided irrevocable evidence showing that viruses augment the metabolisms of bacteria and archaea through expression of auxiliary metabolic genes (AMGs). Several studies have shown that AMGs affect the biogeochemical recycling of sulphur and nitrogen but comparatively less is known regarding their influence on phosphorus recycling.ResultsHere, we provide the first insights regarding the potential effects of phosphorus limitation and AMGs on putative prokaryotic hosts in the euphotic zone of the South Atlantic Ocean (SAO). We identified 7,176 viral contigs that were clustered into 5,999 viral operational taxonomic units (vOTUs, >5kb). These SAO viral communities appear to be unique, as over 89% had no taxonomic assignment, possibly due to the genetic endemism in this ocean. Three phosphatases, phoN, gmhB and rnhA-cobC, were identified as P-cycle AMGs in both prokaryotic double-stranded DNA viruses and eukaryotic Nucleocytoplasmic Large DNA viruses. These genes are associated with the acquisition of inorganic phosphate from phosphate esters, the largest reservoir of P-containing compounds in the marine environment. AMGs were identified in both uncultured and unclassified prokaryotic double-stranded DNA viruses predicted to infect Bacteriodetes, Proteobacteria, Chloroflexota and Poseidonales lineages. ConclusionTogether, these results suggest that viruses modulate P-cycling in euphotic zones of the ocean and that the acquisition of these phosphatase genes may be cues of P-ester stress.
MIMO-Filtered OFDM System Improvement Using Modified Concatenated RS/LDPC Codes
Authors: Hussain Ghasan Ali 
Published: November 2021

Abstract
In mobile communication systems, there are errors that will be generated in the digital signal due to fading and interference. Consequently, different techniques are used to improve the system's reliability and enhance the signal's robustness. Channel coding techniques are used to enhance the system reliability of 5G wireless communication systems . In the upcoming wireless technologies, LDPC codes are still introduced as an alternative to turbo codes. However, the error floor phenomenon is one of the biggest demerits of using LDPC code in the different communication systems that need low error rates. This paper uses RS codes with LDPC codes in a concatenated code to solve this demerit of LDPC codes. Meanwhile, a modified concatenated RS/LDPC codes are created using outer RS codes with inner LDPC codes then appended by interleaver, unlike the conventional concatenated codes that use the interleaver between both codes. Thereafter, the modified concatenated RS/LDPC codes were suggested to enhance BER performance for the f-OFDM system. The results showed that using the proposed concatenated code outperformed using single and familiar concatenated RS/LDPC code in terms of improving BER performance. Meanwhile, the proposed system achieved lower OOBE values than the conventional OFDM system. Therefore, the resulted system can be introduced as a competitor candidate for 5G wireless communication systems due to these features
Response of Human Alveolar Epithelial (hAELVi) Cells to Heavy Metals in Airborne Particulate
Authors: Chang Hui-Fang , Cheng Ji-Yen 
Published: November 2021

Abstract
In vitro human alveolar models provide a platform to investigate the cellular behavior and activity of human lung cells for toxicity assays. However, there has been relatively rare research on the new human alveolar epithelial cell line (hAELVi) for the toxicity induced by heavy metal ions. In this study, hAELVi cells were exposed to various concentrations of Cd, As, and Zn for 24h. The morphological changes of hAELVi cells were observed after exposure to heavy metals. A high concentration of Cd, As, and Zn led to cell death. However, the cytotoxicity was not reflected by the transepithelial electrical resistance (TEER) value after exposure to 250 μM of As and Zn. In addition, Cd at a concentration of 200 μM induced the significant production of TNF-α whereas As and Zn did not induce observable secretion of the cytokine. On the contrary, a high concentration of Cd, As, and Zn inhibited the production of IL-6. In addition, there is not a direct relationship between the metal ion cytotoxicity and the ROS production in the hAELVi cells. Accordingly, this study contributes to elucidating the cytotoxicity, TEER variation, ROS production, and cytokine secretion of hAELVi cells to acute exposure to heavy metal in vitro. The discrepancy between the cytotoxicity and the TEER result suggests that hAELVi cell cultured on permeable membrane exhibit very different behavior, which is worth pursuing.
MicroRNA-342-3p lOaded by Human Umbilical Cord Mesenchymal Stem Cells-derived Exosomes Attenuates Deep Vein Thrombosis by Downregulating EDNRA Running Title: Exosome Delivery of miR-342-3p in DVT
Authors: Pan Zhiyu , Chen Qian , Ding Hongjian , Li Huaqing 
Published: November 2021

Abstract
Objective: Exosomes (exos) exert biological functions to maintain the dynamic balance of cells and tissues by transferring biological cargo to recipient cells. Thus, this study explored human umbilical cord mesenchymal stem cells (hucMSCs)-derived exo transfer of microRNA (miR)-342-3p in deep vein thrombosis (DVT).Methods: HucMSCs were isolated and transfected with miR-342-3p antagomir/agomir. Then, hucMSCs-exos were extracted and injected into rats with DVT to observe inflammation and pathological damage in thrombotic vein. HucMSCs-exos were co-cultured with human umbilical vein endothelial cells (HUVECs) in vitro to observe angiogenesis. miR-342-3p and endothelin A receptor (EDNRA) expression in rats with DVT, as well as their interaction was analyzed.Results: miR-342-3p was downregulated and EDNRA was upregulated in rats with DVT. HucMSCs-exos induced the repair of venous thrombosis and suppressed inflammation and pathological injury in rats with DVT, as well as promoted angiogenesis of HUVECs. Upregulated miR-342-3p delivery by hucMSCs-exos alleviated DVT in rats and improved angiogenesis of HUVECs, while downregulated miR-342-3p performed oppositely. miR-342-3p targeted EDNRA, and the effect of hucMSCs-exos transfer of upregulated miR-342-3p was rescued by overexpressing EDNRA.Conclusion: Briefly, miR-342-3p loaded by hucMSCs-exos attenuates DVT by downregulating EDNRA, offering a novel direction to treat DVT.
Cross Talk Between Down Regulation of Steroidogenic Genes Expression, Oxidative and Apoptotic Biomarkers in Testes Induced by Administration of Tramadol and Boldenone and Their Combination in Male Albino Rats
Authors: Asaad Gihan F. , Mowaad Noha , El-Shamarka Marwa E.A. , Khalil Sahar 
Published: November 2021

Abstract
BackgroundThe testis is the male reproductive gland or gonad having two vital functions - to produce both sperm and androgens, primarily testosterone.PurposeThe study aimed to investigate the effect of tramadol and boldenone injected alone or in combinatio for 2 months in rats on testicular function.MethodsGroup 1; normal control, Group 2; tramadol Hcl (TRAM) (20 mg/kg bwt.) (i.p). Group 3; boldenone undecylenate (BOLD) (5 mg/kg bwt) (i.m). Group 4; combination of TRAM (20 mg/kg bwt.) and BOLD (5 mg/kg), respectively for 2 months.ResultsTRAM and BOLD alone and in combination rats showed deteriorated testicular functions, lowered serum steroid levels (FSH, LH and testesterone), elevation in oxidative biomarkers (MDA & NO) and reduction in GSH and SOD, downregulation of StaR and HSD17B3 as well as assessment of testicular histopathological using H&E staining, PAS stain for histochemical assessment of polysaccharides and glycoproteins in the testes and Masson trichrome stain to assess the changes in the collagen fibers.ConclusionThe study illuminated the hazard of administration of these drugs for a long period as well as the prominent deleterious effects reported on concurrent use of both drugs.