bluuebunny/crossref_metadata_2025_split huggingface dataset:
The influence of primary thyroid cancer to the prognosis of breast cancer: A SEER-based study
Authors: Chen Xinyue , Li Juanjuan , Wu Qi , Liu Hanqing , Yuan Jingping , Sun Shengrong 
Published: March 2021

Abstract
Purpose: Previous studies have revealed a potential link between breast cancer (BC) and thyroid cancer (TC). This study aimed to estimate the clinical characteristics of BC patients who also had primary TC, and to determine the effect of the sequence of the two malignancies on survival. Methods: We selected female patients with BC or TC in the Surveillance, Epidemiology, and End Results (SEER) database (2000 to 2012), and classified them as four cohorts, BC patients with a second TC (BC2TC cohort), TC patients with a second BC (TC2BC cohort), BC patients without TC (BC cohort) and TC patients without BC (TC cohort). Differences in clinical characteristics were analyzed using the chi-square test. The propensity score matching (PSM) method was performed to balance the baseline characteristics. The survival outcomes were analyzed using the Kaplan-Meier log-rank test. Multivariate Cox regression analyses were used to identify risk factors associated with overall survival (OS) and disease-specific survival (DSS). Results: After PSM, 1,260 pairs of patients with or without second primary TC were included in the survival analysis. The patients in BC2TC cohort had better OS (HR: 0.511(0.421-0.620), P<0.001) and DSS (HR: 0.458(0.346-0.606), P<0.001) than those in BC cohort. The subgroup analysis showed that OS was better in the BC2TC cohort in Luminal A and Luminal B subtypes (P<0.05). Conclusion: We found a second primary TC may improve the prognosis of BC patients, especially in women with Luminal breast cancer, while there was no such trend in TC patients with second BC.
Hoarseness After Shoulder Surgery in Beach-chair Position
Authors: Youn Seung-Min , Rhee Sung-Min , Kim Hwan Jin , Lee Hyun Woo , Moon Seong Cheol , Cho Soonghwan , Rhee Yong Girl 
Published: March 2021

Abstract
Background Isolated vocal cord palsy resulting hoarseness after shoulder surgery in beach-chair position had not been reported in literature to date. The purpose of this study was to review its incidence in our patient cohort, and identify any risk factors that may predispose the patient to the injury.Methods There were 10215 operative shoulder cases from January 2010 to December 2017. Inclusion criteria was any post-operative patients, whose operation was performed under general anesthesia in beach-chair position, who had the related symptoms, but the diagnoses had to be confirmed by otorhinolaryngologists with laryngoscopy studies. The affected patients’ clinical notes were retrospectively reviewed with the particular interest in the operative times, and the peri-operative cervical spine radiographs. The degree of cervical spine lordosis was assessed using a method described in literature, in which ‘absolute rotation angle’ (ARA) was measured. Results There were 8 reported cases of vocal cord injury in total (0.08%). Four were male patients and four were arthroscopic cases. The mean age was 59.4 ± 11.9 years old. No particular difficulties with positioning or intubation were documented. The average duration of anesthetic times was 141 minutes. On peri-operative cervical spine radiographs, the average lordosis was 8.2° (1.5° kyphosis - 21° lordosis), and except for one patient, all had ‘non-lordotic’ type curvatures. All but one patient had recovered fully with observation and expectant management, with the average recovery time being 19 weeks (range: 2 weeks to 1 year). Only patient who had not recovered during our 2-year follow-up period, had a ‘sigmoidal’ type cervical spine and was also managed with observation only.Conclusions The incidence of vocal cord injury with beach-chair positioning at our institution was low at 0.08%. The possible risk factors include long duration of the procedure and ‘non-lordotic’ cervical spine, as demonstrated by the trend in our study. Although rare, vocal cord injury has varying duration of recovery time, in the worst-case scenario being permanent, therefore it needs to be avoided by taking utmost care during positioning of the patient in beach-chair. Level of Evidence Level IV, case series
The Treatment of Anxiety in Patients with Somatoform Dysfunction, Reaction to Severe Stress and other Neurotic Disorders: A Multicenter Double-blind Placebo-controlled Randomized Trial
Authors: Parfenov Vladimir , Kamchatnov Pavel , Amelin Aleksandr , Barantsevich Evgeny , Khasanova Dina , Bogdanov Enver , Lokshtanova Tatiana , Maslova Natalya , Pizova Natalya , Kolokolov Oleg , Belskaya Galina , Kamenova Saltanat , Duchshanova Gulsum , Glazunov Alexey 
Published: March 2021

Abstract
The existing treatment of somatoform dysfunction (SD), reaction to severe stress (RSS) and adjustment disorders (AjD) is insufficiently effective and safe. Anxiolytic drug Tenoten (Materia Medica Holding) is shown to be effective in clinical trials (CT). Objectives The aim of multicenter double-blind placebo-controlled randomized CT was to investigate the safety and efficacy of Tenoten in the anxiety treatment of adults with SD, RSS, AjD and other neurotic disorders (oNDs). Methods 390 adults with SD, RSS and AjD or oNDs with the Hospital Anxiety and Depression scale-anxiety (HADS-A) score≥11 were randomized into 4 groups (Tenoten group 14 tablets/day n=127; Tenoten group 3 8 tablets/day n=131, combined Placebo group 2+4 n= 132). The changes from baseline in the mean Hamilton Anxiety Rating Scale (HAM-A) score in groups 1 and 3 after 12 weeks was the primary outcome. Results The decrease in the HAM-A score from 18.81±5.81 to 7.26±4.63 (group 1) and from 18.38±4.3 to 6.40±4.02 (group 3) was observed post-treatment (p group 1/placebo =0.0055, p group 3/placebo <0.0001). The mean changes in the scores in the groups 1, 3 and the Placebo were 11.25, 11.91 and 9.71, respectively. In total, 46 AEs (28 AEs in the Tenoten groups, 18 in the Placebo) were registered in 37 patients (20 in the Tenoten groups, and 17 in the Placebo). No differences in frequency of AEs between groups were found. Conclusions Tenoten was shown to be significantly more effective than placebo in the anxiety treatment of adults with SD, RSS, AjD and oNDs (clinicaltrials.gov NCT03036293).
Validation of the medium and short version of CENSOPAS-COPSOQ: A psychometric study in the Peruvian population
Authors: Lucero-Perez Martha R. , Sabastizagal Iselle , Astete Jhon , Burgos Miguel Angel , Villarreal-Zegarra David , Moncada Salvador 
Published: March 2021

Abstract
Background: The presence of psychosocial risks at work is associated with mental and physical health issues in workers. The study aim was to adapt the COPSOQ-ISTAS21 Medium-Version to the Peruvian context and to develop a Short-Version of the instrument.Method: Cross-sectional design study. The COPSOQ-ISTAS21 Medium Version was used. A confirmatory factor analysis was performed to determine the internal structure of each subdimension (first-order) and dimension (second-order) using the Robust Maximum Likelihood estimation method, and classic fit indices in the literature (CFI, SRMR, RMSEA). Internal consistency was evaluated using the alpha and omega coefficients. A short version was developed based on the items with the highest factorial load and that reduce the factorial complexity.Results: A total of 1707 participants were evaluated. In the confirmatory factor analysis, the goodness-of-fit indices for seventeen of the 20 one-dimensional models (subdimensions) were identified; two subdimensions could not be evaluated because they presented only two items. When conducting a multidimensional analysis, we identified that all second-order models presented optimal goodness-of-fit indices, except “psychological demands at work”. The latter required adding four pairs of correlated errors to reach adequate fit values. Finally, a short version of only 31 items was designed from the items with optimal fit indices.Conclusions: The new adapted versions of COPSOQ-ISTAS21 were renamed CENSOPAS-COPSOQ. The CENSOPAS-COPSOQ is an instrument with sufficient evidence of validity and reliability in its medium and short version, which is why its use is recommended in Peruvian work centers to identify the evaluation and prevention of psychosocial risks at work in Peru.
Nutritional Composition and Mineral Elements Content Analyses of Lonicera fulvotomentosa Hsu et S. C. Cheng Grown in China
Authors: Jiang Xianghui , Xiao Longqian 
Published: March 2021

Abstract
Lonicera fulvotomentosa Hsu et S. C. Cheng (L. fulvotomentosa), a vine shrub found in Southwestern China, is used for treating epidemic fever and infectious diseases, such as SARS and Avian Influenza. Here, we investigated the chemical composition and nutritional content of dried flowers of L. fulvotomentosa grown in yellow loam and Karst landform soil in Guizhou, China. The moisture content in all samples varied from 3.25 to 3.63%, lipids from 7.76 to 9.93%, fiber from 6.93 to 7.34%, ashes from 12.32 to 12.76%, crude protein from 7.85 to 8.53%, and carbohydrates from 56.21 to 59.77%. Using inductively coupled plasma-mass spectroscopy (ICP-MS), the predominant mineral elements in the dried flowers were found to be calcium (297.34-351.26 mg/kg), potassium (132.56-140.37 mg/kg), iron (37.77–41.25 mg/kg), and magnesium (9.47–11.36 mg/kg). Also, HPLC identified flavonoids (kaempferol, rutin, quercetin, luteolin, and apigenin) and phenolic acids (caffeic acid, gallic acid, and chlorogenic acid). Thus, the chemical composition of L. fulvotomentosa was similar to that of Lonicera japonica Thunb. (L. japonica). Thus, it could be used as an alternative to L. japonica. Our results showed that the dried flower of L. fulvotomentosa had an extremely high content of chlorogenic acids and caffeic acid, which could be developed as a candidate molecule as HIV inhibitors.
Do Gibbons Live in Dense Forest? A Case Study from Upper Brahmaputra Valley, Assam, India
Authors: Sarma Kuladip , Saikia Malabika Kakati , Sarania Bidyut , Basumatary Himolin , Baruah Siddhartha Sankar , Saikia Bhrigu Prasad , Kumar Awadhesh , Saikia Prasanta Kumar 
Published: March 2021

Abstract
The present study aimed at predicting the potential habitat of Western Hoolock Gibbon (Hoolock hoolock) in the upper Brahmaputra river valley, Assam and to identify the conservation priority areas for systematic conservation of the species taking canopy cover into account. We used the maximum entropy algorithm for the prediction of the potential habitat of Gibbons using its current distribution with 19 environmental parameters as primary predictors. Spatio-temporal analyses of the habitat were carried out using satellite-based remote sensing and GIS techniques for two decades (1998–2018) along with Terra Modis Vegetation Continuous Field product to examine the land use land cover (LULC), habitat fragmentation, Normalized Difference Vegetation Index (NDVI) and tree cover percentage of the study area. To identify the conservation priority area, we applied a cost-effective decision-making analysis using systematic conservation prioritization in R programming. The model predicted an area of 6025.1 km2 under high potential habitat, and a major part of it was found to overlap with the dense forest (80.04%) followed by moderately open forest (73.90%) and open forest (65.82%). The LULC change matrix showed deduction of forest areas in the predicted high potential habitat during the study period, while agricultural class showed an increasing trend. The fragmentation analysis indicated that the number of patches and patch density increased from 2008 to 2018 in the ‘very dense’ and ‘dense’ canopy regions of the gibbon habitat. Based on the conservation priority analysis, 640 km2 area has been proposed to conserve a minimum 10% of the gibbons’ habitat. The current analysis revealed that in Upper Brahmaputra Valley most of the areas under dense forest and dense canopy have remained intact over the last two decades, at least within the high potential habitat of gibbons independent of the degree of area change in forest, agriculture and plantation.
One-minute Sit-to-stand Test Is Practical to Assess and Follow the Muscle Weakness in Cystic Fibrosis.
Authors: Hardy Sophie , Berardis Silvia , Aubriot Anne-Sophie , Reychler Gregory , Gohy Sophie 
Published: March 2021

Abstract
Background: Quadriceps muscle weakness and reduced exercise tolerance are prevalent in patients with cystic fibrosis (CF) and associated with a worse prognosis. The one-minute sit-to-stand test (1STST) has been proposed to evaluate exercise capacity and assess quadriceps strength. The aim of the study was to correlate the 1STST and the maximal isometric voluntary contraction of the quadriceps (MVCQ) evaluated by the dynamometer in stable patients with CF and to evaluate the impact of intravenous (IV) antibiotherapy.Methods: MVCQ and exercise capacity (dynamometer and 1STST) were measured in stable patients with CF at a routine visit, at the admission and the discharge of an IV antibiotherapy. Patients wore an activity monitor during 72h during IV treatment. Results: 51 stable patients with CF at a routine visit and 30 treated with IV antibiotherapy were recruited. In stable patients, the number of repetitions was reduced to 2101nxkg±657 (mean±SD), representing 79% of the predicted values (%PV) (7;142) (median (min;max)) as well as the MVCQ to 78.64N-m (23.21;170.34), representing 57%PV±26. The 1STST was correlated to MVCQ (r=0.536;p<0.0001) and lung function (r=0.508;p=0.0001). Over the IV antibiotherapy course, the 1STST improves significantly like lung function and body mass index while a positive trend for MVCQ was observed. The gain of 1STST was correlated to the change in MVCQ (r=0.441;p=0.015) and was significantly higher in hospitalized patients versus home therapy.Conclusions: The 1STST is a good alternative to the dynamometer to evaluate and assess muscular weakness for the routine visit and IV antibiotherapy.
Effect of Napsin A and Circulating Tumor Cells with Mesenchymal Phenotype in Lung Adenocarcinoma
Authors: Wei Yu Hong , He yi Zhi 
Published: March 2021

Abstract
Objective: To evaluate the clinical significance of Napsin A and circulating tumor cells（CTCs）with mesenchymal phenotype (M-CTC) in lung adenocarcinoma(LUAD).Materials and Methods: Clinical data of 97 LUAD patients were retrieved. The CanPatrolTM CTC enrichment platform was used to isolate CTCs from the peripheral blood of LUAD patients. The protein expression of Napsin A in the tumor tissues was analyzed by immunohistochemistry. Results: 20 of the 97 patients (20.62%) were negative expression of Napsin A （Napsin A-）and 60 (61.86%) were M-CTC positive（M-CTC+). Both Napsin A expression (P=0.004) and M-CTC+ (P<0.001) showed significant correlation to lymphatic metastasis, and M-CTC+ was also significantly correlated with the tumor stage (P=0.009) but was not correlated with gender, age, smoking, tumor size and degree of differentiation. Furthermore, the Napsin A- patients had a higher positive rate of M-CTC. In addition, the recurrence-free survival (RFS; Log-rank P <0.001) and overall survival (OS; Log-rank P <0.001) of the M-CTC+ LUAD patients were significantly worse. Likewise, Napsin A- was also associated with poor RFS (Log-rank P <0.001) and OS (Log-rank P = 0.0003).Conclusion: LUAD patients with Napsin A- have a higher frequency of M-CTC+, and the Napsin A- and M-CTC+ status portends poor prognosis.
Double-Image Visually Meaningful Encryption Algorithm Based on Compressed Sensing and Frft Embedding
Authors: jiang donghua , Liu Lidong , Zhu Liya , Wang Xingyuan , Chen Yingpin , Rong Xianwei 
Published: March 2021

Abstract
The transmission of images via the Internet has grown exponentially in the past few decades. However, the Internet considered as an insecure method of information transmission may cause serious privacy issues. In order to overcome such potential security issues, a novel double-image visually meaningful encryption (DIVME) algorithm conjugating quantum cellular neural network (QCNN), compressed sensing (CS) and fractional Fourier transform (FRFT) is proposed in this paper. First, the wavelet coefficients of the two plain images are scrambled by the Fisher-Yates confusion algorithm, and then compressed by the key-controlled partial Hadamard matrix. The final meaningful cipher image is generated by embedding the encrypted images into a host image with the same resolution of the plain image via the FRFT-based embedding method. Besides, the eigenvalues of the plain images are utilized to generate the key stream to improve the ability of proposed DIVME algorithm to withstand the plaintext attacks. Afterwards, the plaintext eigenvalues are embedded into the alpha channel of the meaningful cipher image under control of the keys to reduce unnecessary storage space and transmission costs. Ultimately, the simulation results and security analyses indicate that the proposed DIVME algorithm is effective and can withstand multiple attacks.
Terahertz Time-domain Spectroscopy as a Novel Tool for Crystallographic Analysis in Cellulose: the Potentiality of Being a New Standard for Evaluating Crystallinity
Authors: Wang Han , Tsuchikawa Satoru , Inagaki Tetsuya 
Published: March 2021

Abstract
Given that terahertz (THz) radiation responds to intermolecular forces such as hydrogen bonds, THz time-domain spectroscopy (THz-TDS) has expanded possibilities in cellulose research. In this study, THz-TDS was used to investigate the crystallinity of three types of cellulose-based materials. Microcrystalline cellulose (MCC) and wood were ball milled at different times, and pseudo-wood was a mixture of MCC and lignin of different mass fractions. All the samples showed peaks at 3.04 THz in the THz mass absorption coefficient spectra. Further, the spectra from 2.79 THz to 3.32 THz were cut out and detrended by subtraction from a baseline. The integrated intensity of the detrended spectra showed a correlation with the mass fraction of lignin of the pseudo-wood samples, and ball milling time of the MCC and wood samples. The correlation was similar with the crystallinity index calculated from X-ray powder diffraction. Moreover, the original wood sample without ball milling had an integrated intensity that was about 30% that of the original MCC sample, matching with the cellulose concentration of the wood (about 30% to 40%). We normalized the integrated intensity of 2.79 THz to 3.32 THz into 1 to 0 by a min-max algorithm and proposed a new “index” for evaluating crystallinity.
Diversity of Ascomycetes Mushroom in Para Rubber Plantation, Thailand
Authors: Surawut Saowapha , Nak-aim Sorasak , Kunsook Chutapa , Kamhaengkul Laddawan , Kanjanavas Pornpimon , Yasawong Montri 
Published: March 2021

Abstract
Ascomycetes mushrooms are fungi that produce ascospores in asci and some with perithecia. Not only they have a role of decomposer in ecology but also produced some bioactive compound, anti-microbial activity, and cytotoxicity. This study aims to explore the diversity of ascomycetes mushroom species in para rubber plantations and to identify them by morphological and sequence analysis of the internal transcribed spacer (ITS) region. The results found ascomycetes mushroom consist of Trichoderma pezizoides (RP1, % identity 98.79, DQ835513.1), Daldinia eschscholtzii (RP2, % identity 100, MN310384.1), Cookeina sulcipes (RP3, % identity 98.44, KY094620.1), Cookeina garethjonesii (RP4, % identity 99.06, KY094622.1), Cookeina tricholoma (RP5, % identity 100, KY094619.1) and Xylaria terricola (RP6, % identity 88.42, MF577038.1). Most of the ascomycetes in this study have previously been described in Thailand except Xylaria terricola. Additionally, phylogenetic analysis of ascomycetes mushroom showed high genetic relatedness with reference strains. Therefore, the sequence similarity and phylogenetic analysis confirmed the identity of six ascomycetes mushroom species, and further study of bioactive compound from these mushrooms may be investigated for other applications.
Knockdown of 60S Ribosomal Protein L14-2 Reveals Their Potential Regulatory Roles in Enhancing Drought and Salt Tolerance in Cotton
Authors: shiraku Margaret , Magwanga Richard Odongo , Cai Xiaoyan , Kirungu Joy Nyangasi , Xu Yanchao , Mehari Teame Gereziher , Hou Yuqing , Wang Yuhong , Peng Renhai , Wang Kunbo , Zhou Zhongli , Fang Liu 
Published: March 2021

Abstract
BackgroundCotton is an important economic crop and the primary source of natural fiber. The effects of drought and salt stresses threaten strong fiber and large quantity production. However, due to the ever-changing climatic conditions, plants have evolved various mechanisms to cope with the effects of various stress factors. One of the plant's transcription factors with positive effects in alleviating effects of drought and salt stresses is the Ribosomal protein Large (RPL) gene families. This has prompted the functional characterization of the RPL14B gene previously identified in the QTL region as a candidate gene that responds to stress and initiates mechanisms that enhance stress tolerance. ResultsComprehensive identification and functional analysis were conducted in this study, in which 26, 8, and 5 proteins containing the RPL14B domain were identified in G. hirsutum, G. raimondii, and G. arboreum, respectively. Moreover, Cis-regulatory elements associated with the RPL genes were identified. The Myb binding sites (MBS), Myb, Abscisic acid-responsive element (ABRE), CAAT-box, TATA box, TGACG-motif, and CGTCA-motif responsive to Meja, and TCA- motif responsive to salicylic acid were identified. Validation of the candidate gene through virus-induced gene silencing (VIGS) revealed that the Gh_D01G0234 (RPL14B) knockdown significantly affected the cotton seedling's performance under drought/ salt stress conditions as evidenced by a significant reduction in various morphological and physiological traits. Moreover, antioxidant enzyme levels were significantly reduced in VIGS-plants, with substantially higher oxidant enzyme levels, as evidenced by the higher concentration level of Malondialdehyde (MDA). ConclusionThe results revealed the potential role of the gene, and it can be further exploited to breed climate-smart cotton varieties resilient to drought and salt stress conditions
Single-cell transcriptomic analysis elucidates APOE genotype specific changes across cell types in two brain regions in Alzheimer’s disease
Authors: Belonwu Stella , Li Yaqiao , Bunis Daniel , Rao Arjun Arkal , Solsberg Caroline Warly , Oskotsky Tomiko , Taubes Alice , Grone Brian , Zalocusky Kelly , Fragiadakis Gabriela , Huang Yadong , Sirota Marina 
Published: March 2021

Abstract
Alzheimer’s Disease (AD) is a complex neurodegenerative disease that gravely affects patients and imposes an immense burden on caregivers. Apolipoprotein E4 (APOE4) has been identified as the most common genetic risk factor for AD, yet the molecular mechanisms connecting APOE4 to AD are not well understood. Past transcriptomic analyses in AD have revealed APOE genotype-specific transcriptomic differences; however, these differences have not been explored at a single-cell level. Here, we leverage the first two single-nucleus RNA sequencing AD datasets from human brain samples, including nearly 55,000 cells from the prefrontal and entorhinal cortices. We observed more global transcriptomic changes in APOE4 positive AD cells and identified differences across APOE genotypes primarily in glial cell types. Our findings highlight the differential transcriptomic perturbations of APOE isoforms at a single-cell level in AD pathogenesis and have implications for precision medicine development in the diagnosis and treatment of AD.