bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Prognostic Value of the DNA Repair Gene Signature in Head and Neck Squamous Cell Carcinoma
Authors: Ming Ruijie , Wang Enhao , Wei Jiahui , Shen Jinxiong , Zong Shimin , Xiao Hongjun 
Published: July 2021

PurposeTo construct a prognostic signature composed of DNA repair genes to effectively predict the prognosis of patients with head and neck squamous cell carcinoma (HNSCC).MethodsAfter downloading the transcriptome and clinical data of HNSCC from the Cancer Genome Atlas (TCGA), 499 patients with HNSCC were equally divided into training and testing sets. In the training set, 13 DNA repair genes were screened using univariate proportional hazard (Cox) regression analysis and least absolute shrinkage and selection operator (LASSO) Cox regression analysis to construct a risk model, which was validated in the testing set.ResultsIn the training and testing sets, there were significant differences in the clinical outcomes of patients in the high- and low-risk groups showed by Kaplan-Meier survival curves (P &lt; 0.001). Univariate and multivariate Cox regression analyses showed that the risk score had independent prognostic predictive ability (P &lt; 0.001). At the same time, the immune cell infiltration, immune score, immune-related gene expression, and tumor mutation burden (TMB) of patients with HNSCC were also different between the high- and low-risk groups (P &lt; 0.05). Finally, we screened several chemotherapeutics for HNSCC, which showed significant differences in drug sensitivity between the high- and low-risk groups (P &lt; 0.05).ConclusionThis study constructed a 13-DNA-repair-gene signature for the prognosis of HNSCC, which could accurately and independently predict the clinical outcome of the patient. We then revealed the immune landscape, TMB, and sensitivity to chemotherapy drugs in different risk groups, which might be used to guide clinical treatment decisions.
Nutraceutical Study on Maianthemum atropurpureum, a Wild Medicinal Food Plant in Northwest Yunnan, China
Authors: Xu Li , Wang Yizhou , Ji Yuanyuan , Li Ping , Cao Wujisiguleng , Wu Shibiao , Kennelly Edward , Long Chunlin 
Published: July 2021

Maianthemum atropurpureum (Franch) LaFrankie (Asparagaceae), called nibai in Tibetan or dongka in Drung or zhu-ye-cai in local Chinese, is a wild vegetable consumed by the Tibetan people and other ethnic groups in Northwest Yunnan, China. It is also a traditional medicinal plant used by different linguistic groups for antimicrobial purposes. However the nutritional and phytochemical compositions of this important medicinal food plant have not been well studied previously. In this study, the nutrient content for nibai was determined by the China National Standards (GB) methods, and the phytochemical analysis involved multiple chromatographic and spectral methods including LC-TOF-MS analysis. Dried nibai is a rich source of protein (ca. 24.6%), with 18 of the 21 common amino acids. The amino acid content of nibai can reach up to 17.9/100 g, with the essential amino acids as major contributors, corresponding to 42.3% of the total amino acids. Nibai contains rich mineral elements, dietary fiber, vitamins, β-carotene, carbohydrates, and lipids. The phytochemical content of nibai was examined by conventional isolation strategies, as well as HR-ESI-TOF-MS to detect and identify 16 compounds including nine steroid saponins and seven flavonoids. Among these compounds, uridine, adenosine, guanosine, and β-methyl-6-methyl-d-glucopyranoside were found from the genus Maianthemum for the first time. These results help to demonstrate that the local people’s practice of consuming Maianthemum atropurpureum is reasonable due to its high levels of vitamins, minerals, essential amino-acids, and phytochemicals. Nibai may be further developed in Tibet and surrounding regions, and beyond as a health food, nutraceutical, and/or dietary supplement product.
Molecular Characterization of Novel Family IV and VIII Esterases from a Compost Metagenomic Library
Authors: Park Jong-Eun , Jeong Geum-Seok , Lee Hyun-Woo , Kim Hoon 
Published: July 2021

Two novel esterase genes, est8L and est13L, were isolated and identified from a compost metagenomic library. The encoded Est8L and Est13L had molecular masses of 33,181 and 44,913 Da consisting of 314 and 411 amino acids, respectively, without signal peptides. Est8L showed the highest identity (32.9%) to a hyper-thermophilic carboxylesterase AFEST from Archaeoglobus fulgidus compared to other esterases reported and was classified to be a novel member of family IV esterases with conserved regions such as HGGG, DY, GXSXG, DPL, and GXIH. Est13L showed the highest identity (98.5%) to the family VIII esterase Est7K from the metagenome library. Est8L and Est13L had the highest activities for p-nitrophenyl butyrate (C4) and p-nitrophenyl caproate (C6), respectively, and Est13L showed a broad substrate specificity for p-nitrophenyl substrates. Est8L and Est13L effectively hydrolyzed glyceryl tributyrate. The optimum temperatures for activities of Est8L and Est13L were identical (40 °C), and the optimum pH values were 9.0 and 10.0, respectively. Est13L showed higher thermostability than Est8L. Sephacryl S-200 HR chromatography showed that the native form of Est8L was a dimer. Interestingly, Est13L was found to be a tetramer, contrary to other family VIII esterases reported. Est8L was inhibited by 30% isopropanol, methanol, and acetonitrile; however, Est13L was activated to 182.9% and 356.1%, respectively, by 30% isopropanol and methanol. Est8L showed enantioselectivity for the S-form, but Est13L showed no enantioselectivity. These results show that intracellular Est8L and/or Est13L are oligomeric in terms of native forms and can be used for pharmaceutical and industrial applications with organic solvents under alkaline conditions.
The constitutional court function of the Indonesian state concerning system for the implementation impeachment of the president and/or vice president
Authors: MP H Muhamad Rezky Pahlawan 
Published: July 2021

Impeachment is an accusation or indictment of the President or another country's high officials from his position. Impeachment is not new in the history of Indonesian constitution, but the change in the Constitution has caused a change in the constitutional system as well as related to the mechanism of the dismissal of the President and / or Vice President. how is the Impeachment reviewed globally, the history of impeachment in Indonesia and the implementation of impeachment in other countries, the impeachment process of the president according to the 1945 Constitution of the Republic of Indonesia. The process of impeachment in Indonesia after changing the constitution goes through three stages, namely impeachment in the House of Representatives, the Court The Constitution, and the People's Consultative Assembly.
Keywords: Impeachment, Constitutional Court, Government
Implementation of Police Duties to Support the Implementation of Health Protocols During the Covid-19 Pandemic in Order The Realization of a Conducive Kamtibmas Atmosphere (baubau city case study)
Authors: Mursanto Deddy 
Published: July 2021

Police responsibility is very complex ranging from in public service to supporting security against the security of the State. Seeing or studying the task of the Police to the scale of the Police then the author wants to describe the duties and responsibilities of the National Police mandated by law in carrying out its duties and responsibilities by taking samples, namely baubau city police in controlling the mandate of the Law and information related to the handling of the Covid-19 Pandemic where the city of Baubau was not spared the impact of the pandemic. The purpose of this study is to understand the role of the police in the implementation of kamtibmas during the Covid 19 pandemic in Baubau City. The types of data used in the study are primary and secondary data collected from the interview process and literature studies, which will then be analyzed qualitatively with the steps that have reduced the data. The results of this study concluded the role of Bhabinkamtibmas and Intelkam is very basic and supports all police duties in maintaining public order and looking for basic information about people who do not comply with the recommendations of health protocols in the community during the Current Covid-19 Pandemic and provide input to the community to prevent the emergence of new perpetrators of crimes due to the Covid-19 pandemic by giving input on what should be done by the community.
DIFFERENCES IN AGE CHARACTERISTICS, TUMOR MACROSCOPIC SIZE AND HORMONE PROFILE BETWEEN CLASSIC PAPILLARY CARCINOMA PATIENTS AND FOLLICULAR VARIANTS IN Dr. KARIADI HOSPITAL, SEMARANG
Authors: Nabila Alin , Istiadi Hermawan , Wikanta Edmond Rukmana , Miranti Ika Pawitra , Puspasari Dik 
Published: July 2021

Background: New cases of Papillary Thyroid Carcinoma and Carcinoma patients at Dr. Kariadi Hospital, Semarang is quite high, where the most types are Classical Papillary Thyroid Carcinoma and Follicular Variant Papillary Thyroid Carcinoma. On the other hand, in diagnosing PTC, histopathological examination which is a gold standard sometimes has a subjective value. Therefore, it is necessary to have a correlation with the clinical characteristics of the patient in order to get a correct diagnosis. Aim: Understanding the differences in age characteristics, tumor macroscopic size and hormone profile between Classical Papillary Thyroid Carcinoma and Follicular Variant patients at Dr. Kariadi Hospital, Semarang. Method: Analytic observational research with cross sectional design. The number of samples was 38 medical records in which 18 cases of Classic Papillary Thyroid Carcinoma and 20 cases of Follicular Variant Papillary Thyroid Carcinoma. Data with a nominal scale, namely age were analyzed using the Fisher exact test, while the data with a numerical scale, namely the macroscopic size of the tumor and the hormone profile, were tested for normality of Saphiro Wilk then continued with the Mann-Whitney test. Result: Based on the Fisher exact test, there was significant difference (p = 0,009) between age characteristics and Classical Papillary Thyroid Carcinoma and Follicular Variant. In the Mann-Whitney test there was no significant difference (p = 0.3) between the macroscopic size of the classical papillary carcinoma and follicular variant and there was no significant difference TSHs (p = 0.501) and fT4 (p = 0.953) hormone profiles between Classic Papillary Thyroid Carcinoma and Follicular Variants. Conclusion: There was significant difference between the characteristics of age at diagnosis, and there was no significant difference between the macroscopic size of the tumor and the hormonal profile of Classical Papillary Carcinoma and Follicular Variants in Dr. Kariadi Hospital, Semarang.
COMPARISON OF PLYOMETRICS AND AEROBIC EXERCISES ON THE MEMORY
Authors: Christin Yohana Novelia , Bakhtiar Yuriz , Hardian Hardian , Kumaidah Endang 
Published: July 2021

ABSTRACT
Background: Problems of a person's desire to exercise is still a major highlight in the realm of health, both in Indonesia and in the world. Lack of exercise affects brain work, which is a smaller brain volume in people who are not actively exercising, so doing plyometrics and aerobic exercises can be a solution to these problems because exercise can be easily done for health and increased cognitive abilities.
Aim: To understand which exercises are more effective at improving one's short-term memory.
Methods: This study is a quasi-experimental study with 54 subjects, all of whom are students of the Faculty of Medicine Diponegoro University, aged between 15-25 years. Subjects were divided into three groups, namely the plyometrics group, the aerobic group and the control group. Subjects were selected by purposive sampling method and participated in the measurement of short-term memory at the time before exercise and after exercise for 6 weeks. Statistical analysis in this study using paired t-test and independent t-test.
Results: There was a significant difference in the short-term memory measurement results between the treatment and control groups, but a higher increase was found in the plyometrics group, with p value <0.001.
Conclusion: Plyometrics and aerobic exercises improve short-term memory in students of the Faculty of Medicine Diponegoro University. A more significant improvement was found in the plyometrics group.
Keywords: plyometrics exercise, aerobic exercise, short term memory.
External Validation of Major Adverse Cardiovascular Events’ Predictors in ST-Segment Elevation Myocardial Infarction Patients Undergoing Primary Percutaneous Coronary Intervention
Authors: Raharjo Robert Adrianto , Herminingsih Susi , Ardhianto Pipin , Herry Yan 
Published: July 2021

LATAR BELAKANG: Skor risiko KARIADI merupakan sistem skor dengan rentang skor 0-9; komponennya meliputi kelas Killip, final TIMI flow, total ischemic time, kadar kreatinin darah, kadar glukosa darah, tekanan darah sistolik, dan usia. Skor tersebut dikembangkan untuk memprediksi risiko kejadian kardiovaskular mayor (KKvM) selama rawat inap (gabungan kematian, stroke, urgent revascularization, syok kardiogenik, edema paru akut, atau aritmia) pada penderita infark miokard akut dengan elevasi segmen ST (IMA-EST) yang menjalani intervensi koroner perkutan (IKP) primer, namun performa skor risiko tersebut belum pernah divalidasi secara eksternal.
TUJUAN: Melakukan validasi eksternal terhadap skor risiko KARIADI.
METODE: Penelitian ini merupakan penelitian kohort prospektif pada 109 penderita IMA-EST yang menjalani IKP primer di RSUP Dr. Kariadi dari Januari-November 2020. Sampel penelitian menjalani penilaian skor risiko KARIADI dan follow-up selama rawat inap untuk menentukan ada/tidaknya KKvM. Validasi dilakukan dengan menguji performa kalibrasi [dinilai dengan calibration-in-the-large (alfa), calibration slope (beta), serta plot kalibrasi] dan diskriminasi (dinilai dengan c-statistic dan kurva receiver operating characteristic).
HASIL: Delapan belas pasien (16,5%) mengalami KKvM. Skor risiko KARIADI menunjukkan performa kalibrasi yang kurang baik (alfa -0,39; beta 0,71; plot kalibrasi kurang sesuai) dan performa diskriminasi sedang (c-statistic 0,75; IK95% 0,62-0,87).
KESIMPULAN: Skor risiko KARIADI belum valid dalam memprediksi KKvM selama rawat inap pada penderita IMA-EST yang menjalani IKP primer.

Kata Kunci : Infark miokard akut dengan elevasi segmen ST, intervensi koroner perkutan primer, kejadian kardiovaskular mayor, skor risiko KARIADI, validasi eksternal
Peningkatan Ekspresi PD-L1 pada Squamous Cell Carcinoma Nasofaring Tipe Undifferentiated Non Keratinizing
Authors: Yusuf Franky , Prasetyo Awal , Karlowee Vega , Astuti Meira Dewi Kusuma , Nur Rizki Widya 
Published: July 2021

Peningkatan Ekspresi PD-L1 pada Squamous Cell Carcinoma Nasofaring Tipe Undifferentiated Non Keratinizing

Franky Yusuf1*, Awal Prasetyo2, Vega Karlowee3, Meira Dewi Kusuma Astuti4, Rizky Widya Nur5
1,2,3,4,5Departemen Patologi Anatomik Fakultas Kedokteran Universitas Diponegoro/ RSUP Dr. Kariadi Semarang
Korespondensi : franq_yu@yahoo.co.id
ABSTRAK
LATAR BELAKANG: PD-L1 yang diekspresikan oleh sel tumor pada karsinoma nasofaring (KNF) dapat digunakan oleh sel tumor tersebut untuk menghindari sistem imun penderita. Hal ini dapat mempengaruhi prognosis pasien seperti usia, jenis kelamin, tipe histopatologi, stadium tumor dan kualitas hidup melalui aktivitas imunosupresif. Ekspresi PD-L1 diharapkan dapat menjadi biomarker untuk penilaian faktor prognostik dan imunoterapi.
TUJUAN: Untuk mengetahui apakah terdapat hubungan antara tingkat ekspresi PD-L1 dan faktor prognostik pada KNF.
METODE: Penelitian ini menggunakan studi observasional analitik dengan desain cross sectional. Ekspresi PD-L1 diperiksa secara imunohistokimia dengan menggunakan 61 sampel blok parafin, yang telah terdiagnosis sebagai KNF di laboratorium Patologi Anatomi Rumah Sakit Dr. Kariadi dari bulan Januari 2017 sampai Juni 2020. Data klinis pasien yang digunakan sebagai faktor prognostik yaitu usia, jenis kelamin, tipe histopatologi, stadium tumor dan kualitas hidup. Dilakukan analisis dengan uji Chi-Square untuk mengetahui hubungan antara tingkat ekspresi PD-L1 dengan faktor prognostik.
HASIL: Terdapat hubungan yang bermakna antara ekspresi PD-L1 dan tipe histopatologi (p= 0.001). Tidak terdapat hubungan yang bermakna antara ekspresi PD-L1 dan usia (p=0.405), jenis kelamin (p=0. 41), stadium tumor (p=0.928), dan kualitas hidup (p=0.103).
KESIMPULAN: Ekspresi PD-L1 pada penelitian ini tidak dapat dijadikan sebagai faktor prognostik pada pasien KNF, walaupun tipe histopatologi menunjukkan hubungan yang bermakna, sehingga diperlukan penelitian lebih lanjut dengan sampel yang lebih besar dan periode yang lebih lama.
Kata kunci : Faktor prognostik; Karsinoma nasofaring; PD-L1
Peran Latihan Terapeutik Pada Pasien Wanita 23 Tahun Dengan Lupus Eritematosus Sistemik (LES)
Authors: Rahmatika Rahmatika , Handoyo Rudy 
Published: July 2021

Lupus eritematosus sistemik (LES) merupakan penyakit autoimun yang heterogen yang melibatkan banyak organ dengan gambaran klinis yang beragam. Penyakit ini ditandai oleh produksi antibodi terhadap komponen-komponen inti dan umumnya terjadi pada wanita (rasio 9:1), setelah masa pubertas (pada usia 20-30 tahun) serta dipengaruhi oleh faktor genetika.
Pada LES didapatkan keterlibatan kulit dan mukosa, sendi, darah, jantung, paru, ginjal, susunan saraf pusat (SSP) dan sistem imun. Dilaporkan bahwa pada 1000 pasien LES di Eropa yang diikuti selama 10 tahun, manifestasi klinis terbanyak berturut-turut adalah arthritis sebesar 48,1%, ruam malar 31,1%, nefropati 27,9%, fotosensitivitas 22,9%, keterlibatan neurologik 19,4% dan demam 16,6%. Kami melaporkan pasien LES wanita usia 23 tahun dengan arthritis yang mengakibatkan limitasi lingkup gerak sendi (LGS) bahu dan jari tangan sehingga kesulitan dalam melakukan aktivitas sehari-hari (AKS) menggunakan tangan antara lain menggenggam dan memakai pakaian dalam, gangguan pola jalan akibat kekakuan lutut kiri. serta keterbatasan ambulasi (jarak tempuh) akibat nyeri sendi ekstremitas bawah. Pasien diberikan program latihan aerobik dan LGS ekstremitas bawah menggunakan static ergocycle, latihan LGS ekstremitas atas dengan Mechanical Therapy Unit (MTU) serta latihan motorik halus dan latihan AKS. Setelah satu bulan, pasien mengalami perbaikan dalam menggenggam dan memakai pakaian dalam serta dapat berjalan dalam jarak sekitar 100m tetapi pasien masih berjalan jinjit pada kaki kiri akibat kekakuan lutut dan pergelangan kaki kiri.
Immunoassay interference: Studi kasus Nefritis Lupus kelas IV, Sindroma Mielodisplasia dan Neuropsikiatrilupus
Authors: Nindhita Like Rahayu , Widyaningrum Dian 
Published: July 2021

Latar belakang : Systemic Lupus Erythematosus adalah penyakit kronik inflamatif autoimun dengan manifestasi multiorgan. Pemeriksaan bone marrow punction mengkonfirmasi gangguan hematologi. Imunoassai ANA, anti-dsDNA dan anti Sm mengkonfirmasi imunopatogenesis. Tujuan studi kasus ini adalah untuk menganalisis aspek laboratorium dalam diagnosis dan tatalaksana nefritis Lupus kelas IV dan neuropsikiatri lupus dengan Sindroma Mielodisplasia.
Kasus : Seorang wanita 35 tahun mengeluh lemas. Pemeriksaan fisik : hipertensi, konjungtiva palbebra pucat, ulkus dimulut, echimosis dikulit, pleural friction rub paru kanan, edema pitting ekstremitas. Pemeriksaan laboratorium : peningkatan LDH, hiperlipidemia, hipofibrinogenemia, peningkatan D-dimer, proteinuria, hematuria dan silinderuria. Pemeriksaan ANA dan Sel LE positif. Pansitopenia darah tepi dikonfirmasi pemeriksaan BMP didapatkan gambaran MDS. Pasien mengalami kejang, dikonfirmasi MSCT kepala terdapat infark serebri multipel. Anti ds-DNA positif moderate, C3/C4 rendah namun anti Sm negatif.
Kesimpulan : Berdasarkan data klinis dan laboratoris pasien ini didiagnosis SLE derajat berat, nefritis lupus kelas IV, sindroma mielodisplasia dan neuropsikiatrilupus. Anti Sm negatif kemungkinan disebabkan fenomena “cross reactivity” atau “Hook Effect” sehingga menyebabkan interferensi hasil negatif palsu. Anti Sm negatif tidak dapat menyingkirkan diagnosis SLE
Analisa Perbandingan Rumus Haversine Dan Rumus Euclidean Berbasis Sistem Informasi Geografis Menggunakan Metode Independent Sample t-Test
Authors: Palupi Retno , Yulianna Diyan Ayuk , Winarsih SM Santi 
Published: July 2021

Comparative Analysis of the Haversine Formula and the Euclidean Formula based on Geographical Information Systems which aims to analyze and compare the measurement results of the two formulas whether there are significant differences to the distance measured based on geographic information systems. Research objects used to collect distance data are several bank offices in the city of Solo. The method used in this research is to create a geographic information system application using web-based programming using the PHP (Pearl Hypertext Prepocessor) programming language, with the MySQL database. Measurements were carried out on two formulas, namely the Haversine formula and the Euclidean formula and obtained data in the form of distances in kilometers (km) which were obtained from the program system, then the data was processed using Statistical Product and Service Solution (SPSS) 23 software. is the Independent Sample t-test method. From the data analysis, the conclusion is that there is no significant difference in the calculation of the distance between the Haversine formula and the Euclidean formula. The Independent Sample T Test method is able to analyze the comparison of distance measurements using the Haversine formula and the Euclidean formula does not have a significant difference, meaning that the variants of the two formulas are the same.

Kata Kunci : Haversine formula, Euclidean formula , geographic
information system, Independent Sample t-Test Method
PENYULUHAN GIZI DAN PEMERIKSAAN KADAR HB SERTA KEK PADA REMAJA PUTRI DI BEKASI TIMUR
Authors: Tatag Mulyanto  , Sahrudi  , Eli Indawati  
Published: July 2021

Pendahuluan: Anemia gizi terutama yang disebabkan oleh defisiensi zat besi merupakan kelainan gizi yang paling sering ditemui di negara berkembang dan bersifat epidemik. Anemia gizi umumnya terjadi pada perempuan dalam usia reproduktif dan anak-anak. Keadaan ini membawa efek keseluruhan terbesar dalam hal gangguan kesehatan. Anemia defisiensi besi rentan terjadi pada remaja puteri karena meningkatnya kebutuhan zat besi selama masa pertumbuhan.
Metode: Metode yang digunakan untuk tercapainya tujuan pengabdian masyarakat ini adalah dengan metode ceramah, diskusi dan pemeriksaan kadar Hb dan pengukuran LILA
Hasil: Terjadi perubahan tingat pengetahuan remaja putri secara siginifikan terutama pengetahuan baik, yang awalnya hanya 2% setelah penyuluhan menjadi 54%. Ternyata kejadian anemia dan KEK yag terjadi pada remaja putri disebabkan remaja itu sendiri belum tahu tetang Anemia apalagi KEK.
Kesimpulan: Berhasil meningkatkan pengetahuan baik menjadi diatas 50%, remaja putri di Bekasi Timur mengalami anemia sebesar 28% dan remaja putri di Bekasi Timur yang mengalami Kurang Energi Kronis (KEK) sebesar 34%.