bluuebunny/crossref_metadata_2025_split huggingface dataset:
43. CORRELATION BETWEEN SERUM CREATININE AND HEMOGLOBIN LEVEL IN CHRONIC KIDNEY DISEASE PATIENT WITH HYPERTENSION: STUDY ON PRE-HEMODIALYSIS PATIENT AT RSUD ULIN BANJARMASIN
Authors: Emeralda Pradissa Avia , Adiputro Dwi Laksono 
Published: May 2021

Background:
Chronic kidney disease (CKD) is a failure of kidney function due to structural abnormality that occurs for at least 3 months with or without the decrease in glomerular filtration rate (GFR) of less than 60 ml/min/1.73 m2. Hypertension is the leading cause of CKD and commonly found with prevalence ranges from 60%–90% depends on the stage of CKD. People with CKD often experience anemia as the kidney function worsens. Meanwhile, it has been known that the increase of hemoglobin level will affect to the increase of systolic and diastolic blood pressure.

Objective:
The purpose of this study was to analyze the correlation between serum creatinine and hemoglobin levels in CKD patients with hypertension at RSUD Ulin Banjarmasin.

Methods:
This study is analytical observational with cross sectional approach. The samples of this study consist of 40 pre-hemodialysis patients who meet the inclusion criteria at RSUD Ulin Banjarmasin between July–September 2019. The serum creatinine level and hemoglobin level were compared.

Results:
The result of this study showed the mean level of serum creatinine was 10.31 mg/dL and the mean level of hemoglobin was 7.85 g/dL. The correlation between serum creatinine and hemoglobin level was tested using Spearman correlation test and showed p value = 0.023 and r value = −0.318.

Conclusions:
There is a significant and weak negative correlation between serum creatinine and hemoglobin level in CKD patients with hypertension at RSUD Ulin Banjarmasin.
56. DECAFFEINATED LIGHT ROASTED GREEN COFFEE OR GREEN TEA, WHICH ONE AMELIORATE THE RENIN ANGOTENSIN SYSTEM IN 3T3-L1 ADIPOCYTES?
Authors: Lukitasari Mifetika , Nugroho Dwi Adi , Marlita  , Rohman Mohammad Saifur 
Published: May 2021

Background:
Adipose tissue plays a central role in metabolic syndrome. Adipocyte renin angiotensin system (RAS) is overactivated in metabolic syndrome. Coffee consumption is still debatable in amelioration of metabolic syndrome condition.

Objective:
This study aimed to compare the effect of decaffeinated light roasted green coffee (GCE) and green tea extract (GTE) on RAS amelioration in 3T3-L1 adipocytes.

Methods:
3T3-L1 cells were maintained in DMEM high glucose containing 10% fetal bovine serum and 1% penicillin streptomycin. The cells were seeded into 12 well plate with 2 × 105 cells/well density. At day-3, the over confluent cells were subjected to differentiation by 0.5 mM IBMX, 1 mg/mL insulin, 0.25 mM dexamethasone, and 2 mM rosiglitazone and treated with either GCE or GTE at the dose of 125, 250, 500 mg/mL for 48 hours. Then, the cells were maintained in medium containing 1 mg/ml insulin for 9 days. The cells were harvested and the gene expression of angiotensinogen, AT1 receptor, apelin, and PPARɣ were analysed by RT-PCR. The angiotensin 2 level in culture supernatant was analysed by ELISA.

Results:
The angiotensin 2 level, angiotensinogen, AT1 receptor, apelin and PPARɣ were significantly (p < 0.001) lower in all treatment group compared to that of differentiated 3T3-L1 cells group. In GCE group, the lowest angiotensinogen, AT1 receptor, apelin and PPARɣ gene expression were observed in 250 μg/mL group. Moreover, In GTE group the lowest angiotensinogen, AT1 receptor, apelin and PPARɣ gene expression were observed in 125 μg/mL group. The administration of 250 μg/mL GCE or 125 μg/mL GTE resulted in similar RAS component profile (p < 0.716).

Conclusion:
This study suggested that the administration of 250 μg/mL GCE or 125 μg/mL GTE resulted in amelioration of RAS in 3T3-L1 adipocyte
64. THE USE OF CANDESARTAN PRE-STROKE AND THE STROKE OUTCOME
Authors: Pinzon Rizaldy Taslim , Gulo Lise Insanni 
Published: May 2021

Background:
Stroke is a leading cause of death and disability, where the main risk factor is hypertension. Angiotensin Receptor Blocker (ARB) is the most common drug for stroke prevention in high-risk hypertensive patients.

Aim:
The purpose of this study was to see whether the candesartan pre-stroke use can improve the functional outcomes of post ischemic stroke patients.

Method:
The data were obtained from 191 retrospective observational studies. Data were collected from Stroke Registry and medical record at Bethesda Hospital Yogyakarta in 2014–2016, then analysed univariate, followed by bivariate analysis using chi-square test, independent t-test and fisher exact test for the variable which has actual count (F0), and logistic regression for multivariate analysis.

Result:
One hundred and ninety one samples were systematically reviewed to evaluate the effect of candesartan pre-stroke use on functional outcomes of post ischemic stroke patients in Bethesda hospital Yogyakarta whose range of ages was mostly between 61 and 70 years (30.9%) and were mostly male patients (56.5%). Patients with good functional outcomes (<2) were 79.6% and poor functional outcomes (?2) were 20.4%. The results of bivariate analysis showed that candesartan did not affect the improvement of clinical outcome (OR: 1.806, 95% CI: 0.591–5.519, p: 0.294), and also not better than other angiotensin receptor blocker (p = 0.505, OR = 1.472, 95% CI: 0.470–4.611). The multivariate analysis showed that sex (OR: 0.366, 95% CI: 0.156–0.858, p: 0.021), loss of consciousness (OR: 0.107, 95% CI: 0.021–0.549, p: 0.007), limb weakness (OR: 0.236, 95% CI: 0.067–0.834, p: 0.025), dyslipidemia comorbidity (OR: 2.750, 95% CI: 1.177–6.427, p: 0.019) and aphasia (OR: 0.342, 95% CI: 0.107–1.100, p: 0.072) affected the functional outcome.

Conclusion:
The candesartan pre-stroke use did not improve the functional outcome of post ischemic stroke patient.
65. PREVALENCE AND RISK FACTORS OF HYPERTENSION IN ELDERLY: SECONDARY ANALYSIS INDONESIAN FAMILY LIFE SURVEY (IFLS)
Authors: Turana Yuda , Suswanti Ika , Barus Jimmy F , Suryakusuma Linda 
Published: May 2021

Introduction:
Hypertension is a risk factor of cardiovascular diseases; the prevalence was increasing in line with the older age. The aim of this study was to determine the prevalence and risk factors of hypertension in elderly based on secondary data analysis from Indonesian Family Life Survey 2014.

Methods:
A cross sectional study design with 5145 elderly aged more than 60 years old included in this study after weighted using secondary data analysis Indonesian family life survey in 2014. Sex, education, diabetes, total cholesterol, depression and smoking were independent variables. Hypertension was defined as having mean of systole and diastole more than 140/90 mmHg based on 3 times measurement of blood pressure. Chi-Square and logistic regression test was used for analysis.

Result:
Our study found the majority of subjects were female 2734 (53.1%), and education <9 years 4021 (79.6%). The prevalence of hypertension in elderly was 62.5%. Three hundred subjects (5.8%) having diabetes, high total cholesterol 320 (6.2%), and depression 493 (12%), and current smokers 1647 (32%). Bivariate analysis showed that education <9 years, diabetes, and smoking have a significant association with hypertension (p < 0.05). A multivariate analysis found that diabetes as a dominant factor associated with hypertension in elderly after controlling sex, education, and smoking (p < 0.05; OR: 1.348).

Conclusion:
The prevalence of hypertension in elderly was high. Diabetes was found as a dominant factor associated with hypertension.
74. THE EFFECT OF CAFFEINATED COFFEE ON BLOOD PRESSURE: A SYSTEMATIC REVIEW
Authors: Tuasikal Muhammad Isra , Felani Muhammad Rizky , Fathoni Muhammad Yusuf , Sabara Saga Malela Aria 
Published: May 2021

Background
: Coffee is undoubtly one of the most popular drink in the world, and Caffeine is an important component of coffee. Recent analysis of 14 different specialty coffees in the US stated that the amount of caffeine contained about 72–130 mg per 8 oz (240 ml) of brewed coffee. Caffeine exerts a variety of stimulatory effects upon the central nervous system. It has been suggested that caffeine also may be potentially hypertensive. It is important to define possible risks associated with caffeine intake.

Objective:
To review the published data related to intake of caffeinated coffee on Blood Pressure (BP) and to refer the possible mechanism of its bad effect on BP

Methods:
We performed a comprehensive search on topics that assesses effect of caffeinated coffee on blood pressure from inception up until December 2020.

Results:
We got seven specific studies that assessed the potential effects of caffeine and coffee on blood pressure. Three studies summarized that caffeinated coffee can raise both systolic and diastolic BP, with one of them stated that 250 mg of caffeine (2–3 cups of coffee) as the potential cause. One study showed SBP and DPB was greater after intake of caffeinated than decaffeinated coffee, and it stated that significant increase in brachial DBP over 90 minutes of ingestion of caffeinated coffee. One randomized control trial showed Caffeine raised both SBP and DBP (P < 0.0001) in hypertensive and normotensive baselined population.

Conclusion:
Caffeine has the effect to elevate SBP and DBP. Caffeine may cause persistent BP effects in persons who are regular caffeinated coffee drinkers. Person at risk for hypertension may be the most vulnerable to the BP effects of caffeine, therefore we conclude that hypertension risk status related to caffeinated coffee should take priority in future research specifically in Indonesia.
Impact of Vaginal Estrogen on the Urobiome in Postmenopausal Women With Recurrent Urinary Tract Infection
Authors: Jung Carrie E. , Estaki Mehrbod , Chopyk Jessica , Taylor Bryn C. , Gonzalez Antonio , McDonald Daniel , Shin Jenny , Ferrante Kimberly , Wasenda Erika , Lippmann Quinn , Knight Rob , Pride David , Lukacz Emily S. 
Published: May 2021

Objective
The aim of this study was to describe effects of vaginal estrogen (VE) on the urogenital microbiome in postmenopausal women with recurrent urinary tract infections (rUTIs).

Methods
This is a secondary analysis of 17 participants enrolled in a randomized controlled trial of VE versus placebo on urinary tract infection recurrence in postmenopausal women with rUTIs. Paired clean-catch urine samples were collected at baseline and after 6 months of VE and sequenced using 16S rRNA gene sequencing. Sequence reads were analyzed using Quantitative Insights Into Microbial Ecology 2. Changes in α diversity, β diversity, and differentially abundant genera were measured between paired baseline and 6-month samples and between those with a urinary tract infection at 6 months (failures) and those without (successes).

Results
Of the 17 women, 11 were successes and 6 were failures after 6 months of VE treatment. There was a significant change in α diversity from baseline to month 6 in samples overall (Kruskal-Wallis χ
2 = 3.47, P = 0.037) and in the treatment success group (Yuen T = −2.53, P = 0.035). The increase in relative abundance of Lactobacillus crispatus, Lactobacillus gasseri, and Lactobacillus iners AB-1 was correlated with month 6. A relative bloom of L. crispatus compared with L. gasseri was associated with treatment success (Kruskal-Wallis χ
2 = 4.9, P = 0.0014).

Conclusions

Lactobacillus increases in the urogenital microbiome of postmenopausal women with rUTI after 6 months of VE. However, only the relative increase in L. crispatus specifically may be associated with treatment success.
Antibody Response to COVID-19 vaccination in Patients Receiving Dialysis
Authors: Anand Shuchi , Montez-Rath Maria E. , Han Jialin , Garcia Pablo , Cadden LinaCel , Hunsader Patti , Kerschmann Russell , Beyer Paul , Dittrich Mary , Block Geoffrey A , Boyd Scott D , Parsonnet Julie , Chertow Glenn M 
Published: May 2021

AbstractBackgroundPatients receiving dialysis may mount impaired responses to COVID19 vaccination.MethodsWe report antibody response to vaccination from 1140 patients without, and 493 patients with pre-vaccination SARS-CoV-2 RBD antibody. We used commercially available assays (Siemens) to test remainder plasma monthly in association with vaccination date and type, and assess prevalence of absent total receptor binding antibody, and absent or attenuated (index value < 10) semiquantitative receptor binding domain IgG index values. We used Poisson regression to evaluate risk factors for absent or attenuated response to vaccination.ResultsAmong patients who were seronegative versus seropositive before vaccination, 62% and 56% were ≥65 years old, 20% and 24% were Hispanic, and 22% and 23% were Black. Median IgG index values rose steadily over time, and were higher among the seropositive than in the seronegative patients after completing vaccination (150 [25th, 75thpercentile 23.2, 150.0] versus 41.6 [11.3, 150.0]). Among 610 patients who completed vaccination (assessed ≥14 days later, median 29 days later), the prevalence of absent total RBD response, and absent and attenuated semiquantitative IgG response was 4.4% (95% CI 3.1, 6.4%), 3.4% (2.4, 5.2%), and 14.3% (11.7, 17.3%) respectively. Risk factors for absent or attenuated response included longer vintage of end-stage kidney disease, and lower pre-vaccination serum albumin.ConclusionsMore than one in five patients receiving dialysis had evidence of an attenuated immune response to COVID19 vaccination.Significance statementPatients receiving dialysis face high likelihood of severe COVID19; at the same time, vaccination may be less efficacious, as prior data indicate impaired immune responses to influenza and Hepatitis B vaccination. We found that 22% of patients receiving dialysis had suboptimal responses to vaccination, irrespective of whether or not they had evidence of prior SARS-CoV-2 infection. Poorer health status and longer duration of end-stage kidney disease increased likelihood of suboptimal response. Ongoing vigilance for COVID19 in dialysis facilities and studies of modified vaccination dosing schedules will be critical to protecting patients receiving dialysis.
Genome-wide trans-ethnic meta-analysis identifies novel susceptibility loci for childhood acute lymphoblastic leukemia
Authors: Jeon Soyoung , de Smith Adam J. , Li Shaobo , Chen Minhui , Chan Tsz Fung , Muskens Ivo S. , Morimoto Libby M. , DeWan Andrew T. , Mancuso Nicholas , Metayer Catherine , Ma Xiaomei , Wiemels Joseph L. , Chiang Charleston W.K. 
Published: May 2021

AbstractThe risk of childhood acute lymphoblastic leukemia (ALL) differs across ethnic groups and there exist gaps in our understanding of the genetic risk of ALL as most studies took place in populations of predominantly European ancestries. In an effort to address these limitations, we performed a genetic meta-analysis of ALL in 76,317 participants across four ethnic groups, including 17,814 non-European individuals and 3,482 total cases. We replicated 15 out of 16 previously identified loci associated with ALL in our trans-ethnic analysis. We further identified five novel associations at genome-wide significance, including three novel loci and two secondary associations at previously known loci (17q12 and near CEBPE). The three putatively novel loci (rs9376090 near MYB/HBS1L, rs10998283 near TET1, and rs9415680 near JMJD1C/NRBF2) were previously shown to be associated with multiple blood cell traits and other hematopoietic cancers. Polygenic risk scores constructed from our trans-ethnic meta-analysis showed similar efficacy in independent Latino (LAT) and non-Latino white (NLW) ALL cohorts (AUC ∼ 0.67-0.68) and could partly explain the increased risk of ALL in LAT compared to NLW. Cross-population analysis also showed high but significantly less than 100% genetic correlation between LAT and NLW, suggesting potential differences in the underlying genetic architecture between ethnic groups. In summary, our findings enhance the understanding of genetic contribution to ALL risk across diverse populations and highlight the importance to include multiple ethnic groups in GWAS.Key PointsWe identified three novel loci on childhood acute lymphoblastic leukemia near genes previously associated with multiple blood cell traitsPolygenic risk scores using known and novel risk variants showed similar efficacy in Latino and non-Latino white Americans
Hybridization underlies localized trait evolution in cavefish
Authors: Moran Rachel L. , Jaggard James B. , Roback Emma Y. , Rohner Nicolas , Kowalko Johanna E. , Ornelas-García C. Patricia , McGaugh Suzanne E. , Keene Alex C. 
Published: May 2021

SummaryCompared to selection on new mutations and standing genetic variation, the role of gene flow in generating adaptive genetic variation has been subject to much debate. Theory predicts that gene flow constrains adaptive evolution via natural selection by homogenizing allele frequencies among populations and introducing migrant alleles that may be locally maladaptive1. However, recent work has revealed that populations can diverge even when high levels of gene flow are present2–4 and that gene flow may play an underappreciated role in facilitating local adaptation by increasing the amount of genetic variation present for selection to act upon5–8. Here, we investigate how genetic variation introduced by gene flow contributes to adaptive evolution of complex traits using an emerging eco-evolutionary model system, the Mexican tetra (Astyanax mexicanus). The ancestral surface form of the Mexican tetra has repeatedly invaded and adapted to cave environments. The Chica cave is unique in that it contains several pool microenvironments inhabited by putative hybrids between surface and cave populations9, providing an opportunity to investigate the dynamics of complex trait evolution and gene flow on a local scale. Here we conduct high-resolution genomic mapping and analysis of eye morphology and pigmentation in fish from multiple pools within Chica cave. We demonstrate that hybridization between cave and surface populations contributes to highly localized variation in behavioral and morphological traits. Analysis of sleep and locomotor behaviors between individual pools within this cave revealed reduced sleep associated with an increase in ancestry derived from cave populations, suggesting pool-specific ecological differences may drive the highly-localized evolution of sleep and locomotor behaviors. Lastly, our analyses uncovered a compelling example of convergent evolution in a core circadian clock gene in multiple independent cavefish lineages and burrowing mammals, indicating a shared genetic mechanism underlying circadian disruption in subterranean vertebrates. Together, our results provide insight into the evolutionary mechanisms that promote adaptive genetic variation and the genetic basis of complex behavioral phenotypes involved in local adaptation.
Acquired drug resistance enhances imidazoquinoline efflux by P-glycoprotein
Authors: Pulukuri Anunay J. , Burt Anthony J. , Opp Larissa K. , McDowell Colin M. , Nielsen Amy E. , Mancini Rock J. 
Published: May 2021

AbstractMultidrug-Resistant (MDR) cancers mitigate the action of chemotherapeutics through drug efflux that occurs via ABC (ATP-Binding Cassette) transporters, including P-glycoprotein 1 (P-gp or ABCB1/MDR1). Because Toll-Like Receptor (TLR) agonist immunotherapies elicit abscopal anti-tumoral effects by modulating the activity of bystander tumor infiltrating immune cells, they not only circumvent the neutralizing effects of drug efflux, but could also work in synergy with this process. However, the effect of drug resistance on TLR agonist efflux is largely unknown. We begin to address this by investigating P-gp mediated efflux of model TLR agonists in cancer cell lines before and after acquired drug resistance. First, we used functionalized liposomes to determine that imidazoquinoline TLR agonists Imiquimod, Resiquimod, and Gardiquimod are substrates for P-gp. Next, we created Doxorubicin-resistant cancer cell lines from B16 melanoma, TRAMP prostate, and 4T1 breast cancer and observed that each cell line increased P-gp expression in response to Doxorubicin. Comparing imidazoquinoline efflux in Doxorubicin-resistant cell lines, relative to parent cancer cell lines, we used P-gp competitive substrates and inhibitors to demonstrate that imidazoquinoline efflux occurs through P-gp and is enhanced as a consequence of acquired drug resistance. We found that the most hydrophobic, yet least potent imidazoquinoline (Imiquimod), was the best substrate for efflux. This suggests a new parameter, susceptibility to drug efflux, could be an important consideration in the rationale design of the next generation of TLR agonist immunotherapies that are targeted to cancer cells, yet effect their mechanisms of action by modulating the activity of tumor infiltrating immune cells.
Cell strain-derived induced pluripotent stem cell as a genetically controlled approach to investigating aging mechanisms and viral pathogenesis
Authors: Bifani Amanda Makha , Tan Hwee Cheng , Choy Milly M , Ooi Eng Eong 
Published: May 2021

ABSTRACTThe expansion of the geographic footprint of dengue viruses (DENVs) and their mosquito vectors have affected more than half of the global population, including older adults who appear to show elevated risk of severe dengue. Despite this epidemiological trend, how age and senescence impact virus-host interactions involved in dengue pathogenesis to increase the risk of severe dengue is poorly understood. Herein, we show that conversion of diploid cells with finite lifespan into iPSCs followed by differentiation back into cell strain can be an approach to derive genetically identical cells at different stages of senescence to study virus and aging host interactions. Our findings show that cellular senescence impact the host response to infection and the ensuing outcome. We suggest iPSC-derive cell strains as a potentially useful technical approach to genetically controlled host-virus interaction studies to understand how aging impact viral pathogenesis.
LNetReduce: tool for reducing linear dynamic networks with separated time scales
Authors: Buffard Marion , Desoeuvres Aurélien , Naldi Aurélien , Requilé Clément , Zinovyev Andrei , Radulescu Ovidiu 
Published: May 2021

AbstractWe introduce LNetReduce, a tool that simplifies linear dynamic networks. Dynamic networks are represented as digraphs labeled by integer timescale orders. Such models describe deterministic or stochastic monomolecular chemical reaction networks, but also random walks on weighted protein-protein interaction networks, spreading of infectious diseases and opinion in social networks, communication in computer networks. The reduced network is obtained by graph and label rewriting rules and reproduces the full network dynamics with good approximation at all time scales. The tool is implemented in Python with a graphical user interface. We discuss applications of LNetReduce to network design and to the study of the fundamental relation between time scales and topology in complex dynamic networks.Availabilitythe code and application examples are available at https://github.com/oradules/LNetReduce.