bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effect of Discharge Plan on Outcomes of Patients with Lumbar Disc Herniation Surgery
Authors: Abd- Ella Mabrouka A. , Elsenousy Tahany A. , Abdelatief Dalia A. , Marzouk Samar F. 
Published: April 2021

Context: Discharge planning for patients undergoing lumbar disc herniation surgery is considered a strategy for promoting their home and community outcomes.
Aim: This study aimed to evaluate the discharge plan's effect on patients' outcomes with lumbar disc herniation surgery.
Methods: A quasi-experimental design was utilized to conduct this study. The study was conducted in the neurosurgical ward at El-Demerdash hospital and outpatient clinic at Ain Shams University Hospital, affiliated to Ain Shams University. A purposive sample of 60 patients with lumbar disc herniation surgery was selected according to specific inclusion criteria. The sample size was determined statistically by power analysis considering the total number of patients with lumbar disc herniation surgery in El Demerdash hospital (2016). Tools of the study included patients’ interviewing questionnaire regarding patients’ demographic characteristics, assessment of patients' knowledge, and patients' outcomes assessment tools that include Oswestry disability index, the lower extremity functional scale, and the social dysfunction scale and SF36 to assess psychological outcome.
Results: The present study revealed that the mean age of the patients under study was 39.58±8.69, 60% of them were females, and there was a highly statistically significant difference among patients under study throughout discharge planning phases regarding their knowledge, their level of disability, their lower extremity functional scale, total social dysfunction, and total psychological outcomes pre and at follow-up. There were statistically significant relations between patients' level of knowledge and their outcome pre and three months after implementing the discharge plan.
Conclusion: Application of discharge planning regarding lumbar disc herniation surgery improved patients' outcomes. The discharge plan should be available in the neurosurgical department and clinic for all patients who will be undergoing lumbar disc herniation surgery should be updated periodically.
PENGARUH KESADARAN WAJIB PAJAK, SANKSI PAJAK DAN PELAYANAN PAJAK TERHADAP KEPATUHAN WAJIB PAJAK ORANG PRIBADI PADA KANTOR PELAYANAN PAJAK PRATAMA CILEUNGSI, KABUPATEN BOGOR
Authors: . Muhlis , Trisna Novi 
Published: April 2021

Penelitian ini bertujuan untuk mengetahui dan menganalisis pengaruh kesadaran wajib pajak, sanksi pajak dan pelayanan pajak terhadap kepatuhan wajib pajak orang pribadi pada kantor pelayanan pajak pratama cileungsi kabupaten Bogor. Populasi dari penelitian ini adalah wajib pajak orang pribadi. Penelitian ini menggunakan teknik Accidental Sampling yaitu berjumlah 100 sampel. Hasil penelitian menunjukkan bahwa sanksi pajak berpengaruh dan signifikan terhadap tingkat kepatuhan. Hasil uji t terhadap variabel sanksi pajak (X2) diperoleh thitung sebesar 5,505 uji t untuk variabel sanksi pajak adalah 5,505 > 1,985 dengan tingkat signifikansi 0,000 < 0,5. Hasil nilai Fhitung > Ftabel atau 13,615 > 2,36 secara bersama-sama variabel kesadaran wajib pajak, sanksi pajak dan pelayanan pajak memberi pengaruh yang signifikan terhadap kepatuhan wajib pajak orang pribadi. Hasil uji regresi linier diperoleh Y = 15,072 + 0,030 X1 + 0,607 X2 + 0,030 X3 + e. Hasil uji koefisien determinasi menunjukkan bahwa nilai R Square sebesar 29,8 persen.
PENGARUH VALUE CHAIN TERHADAP KEPUASAN KONSUMEN DENGAN MENGGUNAKAN MEDIASI KEUNGGULAN BERSAING PADA APLIKASI GOJEK
Authors: Munawar Auriza Fashshalna Munawar , Widiyanesti Sri 
Published: April 2021

Indonesia sedang mengalami transformasi pada bidang transportasi akibat perkembangan teknologi yang semakin pesat. Dapat dilihat dari munculnya model transpotasi online pada kota besar di Indonesia, seperti Gojek dan Grab yang berbasis aplikasi baik dalam pemesanan maupun pembayarannya. Terdapat beberapa pilihan dalam persaingan, persaingan secara harga atau persaingan secara value added. Ketika perusahaan sedang menghadapi persaingan yang kompetitif, ada beberapa hal yang harus dilakukan yaitu membuat suatu strategi untuk membuat nilai tambah atau disebut keunggulan bersaing. Salah satu membangun keunggulan bersaing dengan mengadopsi prinsip-prinsip value chain.
Penelitian ini merupakan penelitian kuantitatif dengan analisis data kausal. Pengumpulan data yang dilakukan dengan penyebaran kuisioner. Pengambilan sample pada penelitian menggunakan teknik random sampling. Berdasarkan, perhitungan jumlah sample yang digunakan dalam penelitian ini berjumlah 400 responden yang merupakan konsumen gojek yang pernah menggunakan aplikasi gojek. Pengolahan data dilakukan dengan SEM-PLS dan menggunakan sofware Smart PLS versi 3.0
Hasil penelitian menunjukan value chain memiliki pengaruh positif terhadap keunggulan bersaing, dan juga keunggulan bersaing memiliki pengaruh positif terhadap kepuasan konsumen. Hasil penelitian juga menunjukan bahwa value chain memiliki pengaruh positif terhadap kepuasan konsumen dan juga pada penelitian ini keunggulan bersaing sudah menjadi variable intervening yang baik dalam memediasi hubungan value chain dengan kepuasan konsumen.
Hal yang harus dilakukan pada pihak gojek untuk menciptakan keunggulan bersaing yang membantu meningkatkan kepuasan konsumen dengan memperbaiki pengelolaan pada value chain dan mempertahankan aktivitas yang sudah baik yang telah dilakukan oleh gojek.
Directional Modulation of Exciton Emission Using Single Dielectric Nanospheres
Authors: Fang Jie , Wang Mingsong , Yao Kan , Zhang Tianyi , Krasnok Alex , Jiang Taizhi , Choi Junho , Kahn Ethan , Korgel Brian A. , Terrones Mauricio , Li Xiaoqin , Alù Andrea , Zheng Yuebing 
Published: April 2021

AbstractCoupling emitters with nanoresonators is an effective strategy to control light emission at the subwavelength scale with high efficiency. Low‐loss dielectric nanoantennas hold particular promise for this purpose, owing to their strong Mie resonances. Herein, a highly miniaturized platform is explored for the control of emission based on individual subwavelength Si nanospheres (SiNSs) to modulate the directional excitation and exciton emission of 2D transition metal dichalcogenides (2D TMDs). A modified Mie theory for dipole–sphere hybrid systems is derived to instruct the optimal design for desirable modulation performance. Controllable forward‐to‐backward intensity ratios are experimentally validated in 532 nm laser excitation and 635 nm exciton emission from a monolayer WS2. Versatile light emission control is achieved for different emitters and excitation wavelengths, benefiting from the facile size control and isotropic shape of SiNSs. Simultaneous modulation of excitation and emission via a single SiNS at visible wavelengths significantly improves the efficiency and directionality of TMD exciton emission and leads to the potential of multifunctional integrated photonics. Overall, the work opens promising opportunities for nanophotonics and polaritonic systems, enabling efficient manipulation, enhancement, and reconfigurability of light–matter interactions.
Covalent Chemistry‐Mediated Multimarker Purification of Circulating Tumor Cells Enables Noninvasive Detection of Molecular Signatures of Hepatocellular Carcinoma
Authors: Sun Na , Lee Yi‐Te , Kim Minhyung , Wang Jasmine J. , Zhang Ceng , Teng Pai‐Chi , Qi Dongping , Zhang Ryan Y. , Tran Benjamin V. , Lee Yue Tung , Ye Jinglei , Palomique Juvelyn , Nissen Nicholas N. , Han Steven‐Huy B. , Sadeghi Saeed , Finn Richard S. , Saab Sammy , Busuttil Ronald W. , Posadas Edwin M. , Liang Li , Pei Renjun , Yang Ju Dong , You Sungyong , Agopian Vatche G. , Tseng Hsian‐Rong , Zhu Yazhen 
Published: April 2021

AbstractTranscriptomic profiling of tumor tissues introduces a large database, which has led to improvements in the ability of cancer diagnosis, treatment, and prevention. However, performing tumor transcriptomic profiling in the clinical setting is very challenging since the procurement of tumor tissues is inherently limited by invasive sampling procedures. Here, the feasibility of purifying hepatocellular carcinoma (HCC) circulating tumor cells (CTCs) from clinical patient samples is demonstrated with improved molecular integrity using Click Chips in conjunction with a multimarker antibody cocktail. The purified CTCs are then subjected to messenger RNA profiling by NanoString nCounter platform, targeting 64 HCC‐specific genes, which are generated from an integrated data analysis framework with eight tissue‐based prognostic gene signatures from seven publicly available HCC transcriptomic studies. After bioinformatics analysis and comparison, the HCC CTC‐derived gene signatures show high concordance with HCC tissue‐derived gene signatures from the Cancer Genome Atlas database, suggesting that HCC CTCs purified by Click Chips can enable the translation of HCC tissue molecular profiling into a noninvasive setting.
A Ratiometric Fluorescent Biosensor Reveals Dynamic Regulation of Long‐Chain Fatty Acyl‐CoA Esters Metabolism
Authors: Wang Weibo , Wei Qingpeng , Zhang Jiayuan , Zhang Meiqi , Wang Chuchen , Qu Renyu , Wang Yuan , Yang Guangfu , Wang Jing 
Published: April 2021

AbstractDespite increasing awareness of the biological impacts of long‐chain fatty acyl‐CoA esters (LCACoAs), our knowledge about the subcellular distribution and regulatory functions of these acyl‐CoA molecules is limited by a lack of methods for detecting LCACoAs in living cells. Here, we report development of a genetically encoded fluorescent sensor that enables ratiometric quantification of LCACoAs in living cells and subcellular compartments. We demonstrate how this FadR‐cpYFP fusion “LACSer sensor” undergoes LCACoA‐induced conformational changes reflected in easily detectable fluorescence responses, and show proof‐of‐concept for real‐time monitoring of LCACoAs in human cells. Subsequently, we applied LACSer in scientific studies investigating how disruption of ACSL enzymes differentially reduces cytosolic and mitochondrial LCACoA levels, and show how genetic disruption of an acyl‐CoA binding protein (ACBP) alters mitochondrial accumulation of LCACoAs.
Watching the embryo: Evolution of the microscope for the study of embryogenesis
Authors: Iyer Sharada , Mukherjee Sulagna , Kumar Megha 
Published: April 2021

AbstractEmbryos and microscopes share a long, remarkable history and biologists have always been intrigued to watch how embryos develop under the microscope. Here we discuss the advances in microscopy which have greatly influenced our current understanding of embryogenesis. We highlight the evolution of microscopes and the optical technologies that have been instrumental in studying various developmental processes. These imaging modalities provide mechanistic insights into the dynamic cellular and molecular events which drive lineage commitment and morphogenetic changes in the developing embryo. We begin the journey with a brief history of microscopy to study embryos. First, we review the principles and optics of light, fluorescence, confocal, and electron microscopy which have been key techniques for imaging cellular and molecular events during embryonic development. Next, we discuss recent key imaging modalities such as light‐sheet microscopy, which are suitable for whole embryo imaging. Further, we highlight imaging techniques like multiphoton and super resolution microscopy for beyond light diffraction limit, high resolution imaging. Lastly, we review some of the scattering‐based imaging methods and techniques used for imaging human embryos.
Continuous monitoring of cell transfection efficiency with micropatterned substrates
Authors: Azuaje‐Hualde Enrique , Rosique Melania , Calatayud‐Sanchez Alba , Benito‐Lopez Fernando , M. de Pancorbo Marian , Basabe‐Desmonts Lourdes 
Published: April 2021

AbstractThe effect of cell–cell contact on gene transfection is mainly unknown. Usually, transfection is carried out in batch cell cultures without control over cellular interactions, and efficiency analysis relies on complex and expensive protocols commonly involving flow cytometry as the final analytical step. Novel platforms and cell patterning are being studied to control cellular interactions and improve quantification methods. In this study, we report the use of surface patterning of fibronectin for the generation of two types of mesenchymal stromal cell patterns: single‐cell patterns without cell‐to‐cell contact, and small cell‐colony patterns. Both scenarios allowed the integration of the full transfection process and the continuous monitoring of thousands of individualized events by fluorescence microscopy. Our results showed that cell‐to‐cell contact clearly affected the transfection, as single cells presented a maximum transfection peak 6 h earlier and had a 10% higher transfection efficiency than cells with cell‐to‐cell contact.
Are fiscal policy incentives effective in stimulating firms' eco‐product innovation? The moderating role of dynamic capabilities
Authors: Long Siying , Liao Zhongju 
Published: April 2021

AbstractThis study investigates the effectiveness of fiscal policy incentives—in particular, direct subsidies and tax credits—in stimulating eco‐product innovation among Chinese manufacturing firms. We also examine the moderating effects of dynamic capabilities. The empirical results, which are based on survey data from 265 firms in China, demonstrate that both direct subsidies and tax credits are conducive to firms' eco‐product innovation. In addition, we find that when firms' dynamic integration capabilities are strong, the facilitating effects of direct subsidies and tax credits on eco‐product innovation are likewise strong. Moreover, dynamic reconfiguration capabilities positively moderate the relationship between tax credits and eco‐product innovation. By identifying moderators that influence the effect of fiscal incentives on eco‐product innovation in heterogeneous firms, this study contributes to fiscal policy debates and realizing sustainable development.
miR‐140‐3p inhibits colorectal cancer progression and its liver metastasis by targeting BCL9 and BCL2
Authors: Liu Dingsheng , Chen Chunsheng , Cui Mingming , Zhang Hong 
Published: April 2021

AbstractRecent studies have identified microRNAs (miRNAs) as a compelling novel class of biomarker in colorectal cancer (CRC) development and metastasis. Here, we demonstrated that the level of plasma exosomal miR‐140‐3p in CRC patients was lower than that in healthy controls. The decreased miR‐140‐3p level was also observed in CRC patients with liver metastasis. The expression of miR‐140‐3p in CRC tissues were significantly lower than that in matched normal tissues. Functionally, miR‐140‐3p overexpression suppressed proliferation, migration, invasion, and β‐catenin nuclear translocation, as well as promoted apoptosis in LoVo cells, while inhibition of miR‐140‐3p reversed these cellular processes in HCT 116 cells. Notably, BCL9 and BCL2 were recognized as direct targets of miR‐140‐3p. BCL9 knockdown abrogated miR‐140‐3p inhibitor‐induced effects on HCT 116 cells with decreased proliferation, migration, and invasion. BCL2 knockdown increased apoptosis of miR‐140‐3p inhibitor‐transfected HCT 116 cells. In vivo experiments revealed that miR‐140‐3p overexpression inhibited tumor growth in LoVo xenograft model and diminished metastatic nodules in nude mice liver. Taken together, this work supports that miR‐140‐3p exerts as a tumor suppressor in CRC progression via targeting BCL9 and BCL2, and suggests miR‐140‐3p‐BCL9/BCL2 axis may be applied in miRNA‐based therapy and prognostication of CRC.
EmoDescriptor: A hybrid feature for emotional classification in dance movements
Authors: Bai Junxuan , Dai Rong , Dai Ju , Pan Junjun 
Published: April 2021

AbstractSimilar to language and music, dance performances provide an effective way to express human emotions. With the abundance of the motion capture data, content‐based motion retrieval and classification have been fiercely investigated. Although researchers attempt to interpret body language in terms of human emotions, the progress is limited by the scarce 3D motion database annotated with emotion labels. This article proposes a hybrid feature for emotional classification in dance performances. The hybrid feature is composed of an explicit feature and a deep feature. The explicit feature is calculated based on the Laban movement analysis, which considers the body, effort, shape, and space properties. The deep feature is obtained from latent representation through a 1D convolutional autoencoder. Eventually, we present an elaborate feature fusion network to attain the hybrid feature that is almost linearly separable. The abundant experiments demonstrate that our hybrid feature is superior to the separate features for the emotional classification in dance performances.
Energy‐efficient fuzzy logic‐based cross‐layer hierarchical routing protocol for wireless Internet‐of‐Things sensor networks
Authors: Nasri Mohsen , Helali Abdelhamid , Maaref Hassen 
Published: April 2021

SummaryWith rapid increase of the advancement in wireless technologies, the world is now moving toward smart wireless devices which require the communication and collaboration of the Internet of Things (IoT). The smart devices, which called sensor nodes, enhance the use of the IoT as the essential components of today's smart world. Therefore, the wireless sensor network (WSN) is the most widely used technology enabling IoT scenarios. Using the technology, a huge volume of data needs to be tackled and transmitted carefully via the internet. Since the lifetime of the network is related to the reduced battery capacity of the connected wireless sensor nodes, extending the network lifetime is the main goal to be achieved. Clustering algorithm has a significant effect on the system lifetime for the IoT‐enabled WSN applications. In this paper, a fuzzy‐based routing protocol (FRP‐LEACH) and a cross‐layer routing protocol (CLRP‐LEACH) are proposed based on the clustering topology. The proposed algorithms are designed for the healthcare IoT applications. These algorithms offer several advanced cloud‐based services and facilities to serve patients more effectually and protect the medical and paramedical framework from pandemic illness like the ongoing COVID‐19 pandemic. To address the above‐mentioned concerns, an energy efficient routing protocol based on fuzzy logic is proposed to optimize wireless Internet‐of‐Things sensor networks performance. Simulation results are presented, and they show that the proposed algorithms outperform current cluster based‐routing protocols using a fuzzy logic algorithm in terms of generating stable clusters and extending the network lifetime.
Impacts of permeable interlocking concrete pavement on the runoff hydrograph: Volume reduction, peak flow mitigation, and extension of lag times
Authors: Tirpak R. Andrew , Winston Ryan J. , Feliciano Melissa , Dorsey Jay D. , Epps Thomas H. 
Published: April 2021

AbstractPermeable pavements are implemented to provide at‐source treatment of urban stormwater runoff while supporting vehicular and pedestrian use. Studies on these systems have mainly focused on those treating only direct rainfall and installed atop well‐drained soils which typically provide substantial hydrologic mitigation through exfiltration that may not be representative of more hydrologically taxing conditions. A single lane parking area retrofitted with permeable interlocking concrete pavement in Vermilion, OH, USA was monitored over a 15‐month period to quantify its hydrologic performance under such conditions. The 470 m2 permeable pavement was underlain by silt loam soils and a shallow bedrock layer and treated run‐on from the adjacent 324 m2 asphalt drive lane. Observed data were compared to a calibrated SWMM model developed to simulate the pre‐retrofit conditions of the site (i.e., a completely impervious parking lot). Cumulative runoff volumes were reduced by 43% across all events in the monitoring period compared to a fully impervious parking lot. While median peak flows were reduced by 75%, substantial mitigation was limited to smaller, lower intensity events with longer antecedent dry periods (i.e., non‐flood producing events). The permeable pavement significantly delayed the occurrence of peak flows from the site following peak rainfall intensity by a median 29 min. Results from this study demonstrate that permeable pavements which receive run‐on from adjacent imperious cover and are installed atop poorly drained soils can significantly reduce runoff volumes and peak flow rates and delay the occurrence of peak discharge. The modelling approach implemented can provide a better estimation of diffuse inflows to green infrastructure stormwater controls and aid in refining design features which enhance the hydrologic performance in systems underlain by poorly drained soils.
FOXD1 promotes dedifferentiation and targeted therapy resistance in melanoma by regulating the expression of connective tissue growth factor
Authors: Sun Qian , Novak Daniel , Hüser Laura , Poelchen Juliane , Wu Huizi , Granados Karol , Federico Aniello , Liu Ke , Steinfass Tamara , Vierthaler Marlene , Umansky Viktor , Utikal Jochen 
Published: April 2021

AbstractMetastatic melanoma is an aggressive skin cancer and associated with a poor prognosis. In clinical terms, targeted therapy is one of the most important treatments for patients with BRAFV600E‐mutated advanced melanoma. However, the development of resistance to this treatment compromises its therapeutic success. We previously demonstrated that forkhead box D1 (FOXD1) regulates melanoma migration and invasion. Here, we found that FOXD1 was highly expressed in melanoma cells and was associated with a poor survival of patients with metastatic melanoma. Upregulation of FOXD1 expression enhanced melanoma cells' resistance to vemurafenib (BRAF inhibitor [BRAFi]) or vemurafenib and cobimetinib (MEK inhibitor) combination treatment whereas loss of FOXD1 increased the sensitivity to treatment. By comparing gene expression levels between FOXD1 knockdown (KD) and overexpressing (OE) cells, we identified the connective tissue growth factor (CTGF) as a downstream factor of FOXD1. Chromatin immunoprecipitation and luciferase assay demonstrated the direct binding of FOXD1 to the CTGF promoter. Similar to FOXD1, knockdown of CTGF increased the sensitivity of BRAFi‐resistant cells to vemurafenib. FOXD1 KD cells treated with recombinant CTGF protein were less sensitive towards vemurafenib compared to untreated FOXD1 KD cells. Based on these findings, we conclude that FOXD1 might be a promising new diagnostic marker and a therapeutic target for the treatment of targeted therapy resistant melanoma.
Coronavirus genomic nsp14‐ExoN, structure, role, mechanism, and potential application as a drug target
Authors: Tahir Mohammed 
Published: April 2021

AbstractThe recent coronavirus disease 2019 (COVID‐19), causing a global pandemic with devastating effects on healthcare and social‐economic systems, has no special antiviral therapies available for human coronaviruses (CoVs). The severe acute respiratory syndrome coronavirus 2 (SARS‐Cov‐2) possesses a nonstructural protein (nsp14), with amino‐terminal domain coding for proofreading exoribonuclease (ExoN) that is required for high‐fidelity replication. The ability of CoVs during genome replication and transcription to proofread and exclude mismatched nucleotides has long hindered the development of anti‐CoV drugs. The resistance of SARS‐CoV‐2 to antivirals, especially nucleoside analogs (NAs), shows the need to identify new CoV inhibition targets. Therefore, this review highlights the importance of nsp14‐ExoN as a target for inhibition. Also, nucleoside analogs could be used in combination with existing anti‐CoV therapeutics to target the proofreading mechanism.