bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluation of a Theory-based Mobile Health Physical Activity Intervention for Breast Cancer Patients During Chemotherapy: Mixed Method Study
Authors: Geng Zhaohui , Ning Li , Cai Lingzhi , Liu Ying , Wang Jingting , Zhang Yingting , Wu Fulei , Yuan Changrong 
Published: August 2021

Abstract

Purpose Mobile health (mHealth) application (app) targeted promoting physical activity (PA) demonstrates advantages in knowledge sharing, social connecting and tailored intervention for breast cancer (BC) patients. However, lacking of theoretical basis restricted sustainable benefits of these apps. We construct a theory-based mHealth PA intervention program and to determine whether this would improve PA behavior change during chemotherapy for BC patients.Methods Social cognitive theory, self-efficacy theory and the theory of planned behavior were referred to construct mHealth intervention strategies. Guided by agile development model, “Breast care” smartphone app based on above integrated theories was developed. Then, a three-month pre-post mHealth PA intervention delivered by the app was implemented. A mixed method was utilized to test the effectiveness of this remotely intervening. Results Twenty participants were recruited. Based on PA capability assessment and baseline PA evaluation, 12 patients were divided into active group, and 8 patients were grouped in sedentary lifestyle. Within three months, the average accumulated app usage time of each participant identified at portal site was 40 minutes a week. The total PA increased 945.70 metabolic equivalents (MET) -min/w with a significant improvement (P=.04) after 3 months. Walking displayed a significant improvement after intervention (904.20 MET-min/w) (P=.03). Qualitative results showed satisfaction and willingness of BC patients to use the app to manage PA and relevant health behaviors.Conclusion The theory-based mHealth PA intervention has great potential to enhance BC patients’ PA awareness and engagement, meanwhile to facilitate their PA behavior change.
Surfactant protein C, a modulator of paravascular brain waste clearance, is associated with perineuronal nets and shows age-dependent changes of brain content and characteristic deposits in wildtype and 3xTg mice
Authors: Schob Stefan , Puchta Joana , Winter Karsten , Michalski Dominik , Mages Bianca , Martens Henrik , Emmer Alexander , Hoffmann Karl-Titus , Gaunitz Frank , Meinicke Anton , Krause Matthias , Härtig Wolfgang 
Published: August 2021

Abstract
Surfactant Protein C (SP-C) modulates cerebrospinal fluid (CSF) rheology. During ageing, its declining levels are accompanied by an increased burden of white matter lesions. Pulmonary SP-C intermediates harbouring the BRICHOS-domain prevent protein misfolding in the lungs. Thus, cerebral SP-C intermediates may counteract cerebral β-amyloidosis, a hallmark of Alzheimer’s disease (AD). However, data on the molecular neuroanatomy of SP-C and its alterations in wildtype and triple transgenic (3xTg) mice, featuring essential elements of AD-neuropathology, are lacking. Therefore, this study investigated SP-C-containing structures in murine forebrains and their spatial relationships with vascular, glial and neuronal components of the neurovascular unit. Fluorescence labelling demonstrated neuronal SP-C in the medial habenula, the indusium griseum and the hippocampus. Glial counterstaining elucidated astrocytes in the corpus callosum co-expressing SP-C and S100β. Notably, perineuronal nets were associated with SP-C in the nucleus reticularis thalami, the lateral hypothalamus and the retrosplenial cortex. In the hippocampus of aged 3xTg mice, an increased number of dot-like depositions containing SP-C and Reelin, but devoid of BRICHOS-immunoreactivity were observed apart from AD-like lesions. Wildtype and 3xTg mice revealed an age-dependent increase of such deposits markedly pronounced in about 24-month-old 3xTg mice. SP-C levels of the intracellular and extracellular compartments in each group revealed an inverse correlation of SP-C and Reelin, with reduced SP-C and increased Reelin in an age-dependent fashion especially in 3xTg mice. Taken together, extracellular SP-C, as modulator of glymphatic clearance and potential ligand of PNs, declines in 3xTg mice, which show an accumulation of extracellular Reelin depositions during ageing.
Ataxia-telangiectasia and combined hepatocellular-cholangiocarcinoma: A case report
Authors: Singh Tej Pratap , Patkar Shraddha , Goel Mahesh , Bal Munita 
Published: August 2021

Abstract
Ataxia telangiectasia (A-T) is a rare autosomal recessive disease characterized by ataxia, cutaneous and ocular telangiectasia, impaired immunity with susceptibility to sino-pulmonary infections, radiation sensitivity and cancers particularly of haemato-lymphoid origin. Liver function tests abnormalities and elevated alfa feto-protein have been reported in A-T however there is no reported case of combined hepatocellular-cholangiocarcinoma (cHCC-CC) in literature. These tumours should be treated in similar fashion as in general population however reduction of chemotherapy dose might be helpful in decreasing chemo-toxicity.
Utility of molecular analysis of peritoneal fluid in staging laparoscopy of advanced oesophagogastric junction and gastric cancer prior to neoadjuvant treatment
Authors: Miro Monica , Vives Regina , Farran Leandre , Secanella Lluis , Varela Mar , Baixeras Nuria , Estremiana Fernando , Bettonica Carla , Aranda Humberto , Galán Maica 
Published: August 2021

Abstract
The significance of molecular analysis of peritoneal fluid in staging laparoscopy of oesophagogastric junction (EGJ) and gastric cancer is still controversial. METHODS: A retrospective analysis of prospective data collection of all patients diagnosed with locally advanced gastric neoplasia or Siewert II-III EGJ cancer from July 2009 to October 2019 who underwent staging peritoneal lavage prior to neoadjuvant treatment was performed. Cytology studies and molecular analysis of peritoneal lavage were performed by real-time reverse transcriptase polymerase chain reaction (RT_PCR) of carcinoembryonic antigen (CEA) and cytokeratin 20 (CK20) as tumor makers. RESULTS: 168 patients were operated. Citology and molecular analysis were performed in 138 patients. Macroscopic carcinomatosis (P+) was evidenced in 12.3% (17 patients). Of the remaining 87.7% (121 patients), 9.9% (12 patients) had positive cytology. In 21% (29 patients) disseminated macroscopic or microscopic disease (P + or Cyt+) was identified. 11.6% (16 patients) were P0Cyt- but RT-PCR+. Of these, 9 responded to chemotherapy before salvage surgery. The sensitivity of cytology and molecular analysis was 0.70 and 0.76, respectively (p = 0.67). The survival of Cyt-RT_PCR + vs. Cyt + RT_PCR + patients was similar (p = 0.1). However, there were no differences in the survival curves between Cyt-RT_PCR- patients vs Cyt-RT_PCR + patients who underwent salvage surgery (p = 0.69). CONCLUSION: Molecular analysis showed a sensitivity of 76%. In our study, the survival of P0 Cyt-RT_PCR + patients responding to chemotherapy and undergoing surgery was similar to that of P0 Cyt-RT_PCR- patients but less disease free survival.
Development of the Opioid Self-Management Scale for Advanced Cancer Patients and Examination of its Validity and Reliability
Authors: Yoshida Shiori , Sato Fumiko , Tagami Keita , Sasaki Rie , Takahashi Chikako , Sasaki Konosuke , Takahashi Shin 
Published: August 2021

Abstract
Background: Approximately 60% of outpatients with advanced cancer experience pain, and self-management with opioids according to lifestyle is important for appropriate pain relief. To date, there are no studies that clearly describe the concept of opioid self-management or that have assessed the factors involved, such as improving self-management abilities. This study developed the Opioid Self-Management Scale for Advanced Cancer Patients (OSSA), and examined its validity and reliability.Methods: The scale was developed in three phases. In phase 1 the scale content was validated. In phase 2 surface validity was examined. The surface validity was examined using a draft scale that extracted qualitatively and deductively. Phase 3 validated and verified the reliability of the OSSA. The validity and reliability were examined using a factor analysis and re-testing.Results: The OSSA consists of 33 items on six subscales. The structural equation modeling was such that the χ2 value was 709.8 (p<.001, df = 467), goodness-of-fit index 0.78, adjusted goodness-of-fit index 0.73, root mean squares of approximation 0.063, and comparative fit index 0.92. Cronbach’s α was 0.93. The intraclass correlation coefficient was 0.59–0.90. The coefficient was -0.21 (p<0.05) for the total OSSA score and “Average pain over 24 hours,” and 0.26 (p<0.01) for “Rate of pain relief over 24 hours.”Conclusion: We determined that the OSSA had tolerable validity and reliability, and the results indicated that a higher self-management ability leads to greater pain relief. The OSSA scales can be considered effective for use in research. A shortened version of the OSSA is required for realistic and practical clinical use.
2-chloro-5-(1-hydroxy-3-oxoisoindolin-1-yl)benzenesulfonamide Derivatives as Potential Urease Inhibitors: Synthesis, In-vitro and Molecular Modeling Approach
Authors: Irshad Sajid , Ahmad Saeed , khan Mohsin Abbas , Bilal Muhammad Sajjad , ejaz syeda Abida 
Published: August 2021

Abstract
The authors have requested that this preprint be removed from Research Square.
Glucose and Xylose Productions From Oil Palm Empty Fruit Bunch by Hydrolysis With Enzyme and Acid Using Response Surface Methodology.
Authors: Sinjaroonsak Santat , H-Kittikun Aran , Chaiyaso Thanongsak , Suyotha Wasana 
Published: August 2021

Abstract
Oil palm empty fruit bunch (EFB) is a major cellulosic waste from a palm oil mill. The use of EFB for bioconversion to fuel and valuable products is possible because this biomass is a cheap, renewable and abundantly available. This study was aimed to produce sugars from the alkaline peroxide pretreated EFB (APEFB) by hydrolysis with a commercial enzyme (iKnowzyme acid 2XL cellulase) in comparison with hydrochloric acid. Response surface methodology (RSM) was applied to improve the hydrolysis process. For an enzymatic hydrolysis, the optimum enzyme dose of 40 U/g APEFB and the liquid to solid ratio of 10 ml/g APEFB were investigated at 150 rpm and 50°C for 120 h. After saccharification, glucose and xylose obtained were 65.71 g/l (0.66 g/g APEFB) and 2.14 g/l (0.02 g/g APEFB), respectively. Many acids (acetic, formic, hydrochloric (HCl), nitric, orthophosphoric and sulfuric acids) were used to hydrolyze APEFB. The result showed that HCl was the best acid to produce glucose and xylose from APEFB with low furfural and hydroxymethylfurfural productions. The optimum HCl concentration and temperature for APEFB saccharification were 5.85% (w/v) acid at 114°C for 90 min. The glucose, xylose, furfural, and hydroxymethylfurfural obtained under these conditions were 10.70 g/l (0.11 g/g APEFB), 15.30 g/l (0.15 g/g APEFB), 2.34 g/l (0.02 g/g APEFB) and 0.67 g/l (0.007 g/g APEFB), respectively.
Brewing and Probiotic Potential Activity of Wild Yeasts Hanseniaspora Uvarum PIT001, Pichia Kluyveri LAR001 and Candida Intermedia ORQ001
Authors: Piraine Renan Eugênio Araujo , Retzlaf Gustavo M , Gonçalves Vitória S. , Cunha Rodrigo C , Leite Fabio Pereira Leivas 
Published: August 2021

Abstract
Non-conventional yeasts can be isolated from a wide range of environmental sources, often found in beverage industry in mixed fermentations, in which the microorganisms’ inoculum usually is not fully known. It is important to know starter cultures, since in addition to favoring reproducibility, other properties can be discovered. Thus, the objective of this work was to identify and characterize yeasts isolated from environment, evaluating their probiotic potential and possible use in brewery. Isolates were obtained from flowers, fruits, leaves and mixed-fermentation beers, being identified by PCR. Yeasts with promising activity were evaluated regarding their growth under different pHs, temperature and presence of organic acids. To explore probiotic potential, in vitro tests were performed of antimicrobial activity and co-aggregation with food pathogens, auto-aggregation, and survival in simulated gastrointestinal tract conditions. In our study, Pichia kluyveri (LAR001), Hanseniaspora uvarum (PIT001) and Candida intermedia (ORQ001) were selected among 20 isolates. P. kluyveri was the only one that tolerated pH 2.5. Lactic acid was not inhibitory, while acetic acid and incubation at 37 °C had a partially inhibitory effect on yeasts growth. All yeasts tolerated α-acids from hops and NaCl up to 1%. It is suggested that isolates are able to adhere to intestinal cells and influence positively the organism in combating pathogens, as they showed auto-aggregation rates above 99% and antagonistic activity to pathogenic bacteria. The yeasts tolerated gastric environment conditions, however were more sensitive to pancreatic conditions. We conclude that isolated non-conventional yeasts showed probiotic potential and promising application in beer fermentation.
Machine learning-based evidence and attribution mapping of 100,000 climate impact studies
Authors: Callaghan Max , Schleussner Carl-Friedrich , Nath Shruti , Lejeune Quentin , Knutson Thomas R. , Reichstein Markus , Hansen Gerrit , Theokritoff Emily , Andrijevic Marina , Brecha Robert J. , Hegarty Michael , Jones Chelsea , Lee Kaylin , Lucas Agathe , Maanen Nicole van , Menke Inga , Pfleiderer Peter , Yesil Burcu , Minx Jan C. 
Published: August 2021

Abstract
An ever-growing body of evidence suggests that climate change is already impacting human and natural systems around the world. Global environmental assessments assessing this evidence, for example by the Intergovernmental Panel on Climate Change (IPCC)1, face increasing challenges to appraise an exponentially growing literature2 and diverse approaches to climate change attribution. Here we use the language representation model BERT to identify and classify studies on observed climate impacts, producing a machine-learning-assisted evidence map which provides the most comprehensive picture of the literature to date. We identify 100,724 (62,950 − 162,838) publications covering a broad range of impacts in human and natural systems across all continents. By combining our spatially resolved database with human-attributable changes in temperature and precipitation on the grid cell level, we infer that attributable climate change impacts may be occurring in regions encompassing 85% (80%) of the world's population (land area). Our results also reveal a substantial 'attribution gap' as robust evidence for attributable impacts is twice as prevalent in high income compared to low income countries. While substantial gaps remain on confidently establishing attributable climate impacts at the regional and sectoral level, our unique database illustrates the broad extent to which anthropogenic climate change may already be impacting natural systems and societies across the globe.
Electrolytes, Metabolic And Acid-Base Parameters In Blood And Fluids of The Reproductive Tract During In Vivo Maturation of Bovine Oocytes
Authors: Gungor Omer F. , Salman Saleh , Ranjitkar Saurav , Zhang Delong , Tian Xiuchun (Cindy) 
Published: August 2021

Abstract
Follicular fluid is the microenvironment that supports oocyte maturation and competence. Using Abbott iSTAT1™ and NanoDrop, we determined the dynamics of acid-base, electrolyte, metabolites, and total protein in venous blood, fluids of the dominant follicle (FF), oviduct (OF), and uterus (UF) during the window of oocyte maturation. Holstein heifers (n=36) were synchronized with PGF2α on Days -11 and 0, CIDR during Days -6 to 1, and GnRH given on Day 2 after 2nd PG. Samples were collected at 24h, 48h, 60h, 72h, and 78h after 2nd PG. Most electrolytes analyzed, Cl-, K+, and Ca2+ were significantly affected in blood and FF (P<0.05) by CIDR removal. Similarly, Cl- and Na+ also significantly changed in OF and UF across time. Glucose, lactate, and creatinine significantly changed across time points in FF compared to blood. Moreover, pO2, pCO2, TCO2, and pH significantly changed across time in FF. Most parameters were not significantly correlated between blood and FF across time points except for glucose, Cl- and creatinine. Furthermore, pO2 in FF was nearly 3X higher than blood, suggesting low O2 during in vitro maturation is inappropriate. In conclusion, components of the follicular fluid undergo major changes during the window of oocyte maturation.
A Radar Object-Based Examination of Rain System Climatology and Including Climate Variability
Authors: Ayat Hooman , Evans Jason P. , Sherwood Steven C. , Soderholm Joshua 
Published: August 2021

Abstract
We know the climate is warming and this is changing some aspects of storms, but we have little knowledge of storm characteristics beyond intensity, which limits our understanding of storms overall. In this study, we apply a cell-tracking algorithm to 20 years of radar data at a mid-latitude coastal-site (Sydney, Australia), to establish a regional storm climatology. The results show that extreme storms in terms of translation-speed, size and rainfall intensity usually occur in the warm season, and are slower and more intense over land between ~10am and ~8pm (AEST), peaking in the afternoon. Storms are more frequent in the cold season and often initiate over the ocean and move northward, leading to precipitation mostly over the ocean. Using clustering algorithms, we have found five storm types with distinct properties, occurring throughout the year but peaking in different seasons. While overall rainfall statistics don't show any link to climate modes, links do appear for some storm types using a multivariate approach. This climatology for a variety of storm characteristics will allow future study of any changes in these characteristics due to climate change.
Long-term Fertilization Enhances Soil Carbon Stability by Increase the Ratio of Passive Carbon: Evidence From Four Typical Croplands
Authors: Zhou Wei , Wen Shilin , Zhang Yunlong , Gregory Andrew S. , Xu Minggang , Shah Syed Atizaz Ali , Zhang Wenju , Wu Honghui , Hartley Iain P. 
Published: August 2021

Abstract
Aims Soil organic carbon (SOC) plays an important role in improving soil quality, however how long-term fertilization influences SOC and contrasting active carbon (AC) and passive C (PC) pools at large scale remains unclear. The aim of this study was to examine the effect of long-term fertilization on SOC, including AC and PC, across four typical croplands in China and to explore the potential relationships and mechanism. Methods We assessed the effect of different fertilization (standard and 1.5 × standard of inorganic fertilizer (NPK) with or without manure (M), with a control for comparison) at soil depths (0-20 cm, 20-40 cm, 40-60 cm) on SOC, AC and PC. Results We found that SOC, AC and PC increased in the order Control < NPK < NPKM < 1.5NPKM. 1.5NPKM resulted in a significant increase in SOC, AC and PC, of 76.3%, 53.0% and 108.5% respectively across the soil profile (0-60 cm) compared with Control. The response ratio of PC to long-term fertilization was 2.1 times greater than that of AC across four sites on average. In addition, Clay was identified as the most important factor in explaining the response of AC and PC to different fertilization application, respectively. Conclusions Long-term fertilization enhanced both AC and PC, but the greater response of PC suggests that fertilization application could enhance the stability of carbon and thus the potential of cropland for soil carbon accumulation.
Methanol utilizers of the rhizosphere and phyllosphere of a common grass and forb host species
Authors: Kanukollu Saranya , Remus Rainer , Ruecker Alexander Martin , Tschiskale Caroline Buchen , Hoffmann Mathias , Kolb Steffen 
Published: August 2021

Abstract
Background: Managed grasslands are global sources of atmospheric methanol, which is one of the most abundant volatile organic compounds (VOCs) in the atmosphere and promotes oxidative capacity for tropospheric and stratospheric ozone depletion. The phyllosphere is a favoured habitat of plant-colonizing methanol-utilizing bacteria. These bacteria also occur in the rhizosphere, but their relevance for methanol consumption and ecosystem fluxes is unclear. Methanol utilizers of the plant-associated microbiota are key for the mitigation of methanol emission through consumption. However, information about grassland plant microbiota members, their biodiversity and metabolic traits, and thus key actors in the global methanol budget is largely lacking.Results: We investigated the methanol utilization and consumption potentials of two common plant species ( Festuca arundinacea and Taraxacum officinale ) in a temperate grassland. The selected grassland exhibited net methanol emission. The detection of 13 C derived from 13 C-methanol in 16S rRNA of the plant microbiota by stable isotope probing (SIP) revealed distinct methanol utilizer communities in the phyllosphere, roots and rhizosphere but not between plant host species. The phyllosphere was colonized by members of Gamma - and Betaproteobacteria . In the rhizosphere, 13 C-labelled Bacteria were affiliated with Deltaproteobacteria , Gemmatimonadates, and Verrucomicrobiae. Less-abundant 13 C-labelled Bacteria were affiliated with well-known methylotrophs of Alpha -, Gamma -, and Betaproteobacteria . Additional metagenome analyses of both plants were consistent with the SIP results and revealed Bacteria with methanol dehydrogenases (e.g., MxaF1 and XoxF1-5 ) of known but also unusual genera (i.e., Methylomirabilis , Methylooceanibacter , Gemmatimonas , Verminephrobacter ). 14 C-methanol tracing of alive plant material revealed divergent methanol oxidation rates in both plant species but similarly high rates in the rhizosphere and phyllosphere.Conclusions: Our study revealed the rhizosphere as an overlooked hotspot for methanol consumption in temperate grasslands. We also identified unusual methanol utilizers in the phyllosphere and rhizosphere. We did not observe a plant host-specific methanol utilizer community. Our results suggest a model for methanol turnover in which both the sources (plants) and sinks (microbiota) of a volatile are separated but in the same ecological unit.