bluuebunny/crossref_metadata_2025_split huggingface dataset:
Pseudomonas syringae effector AvrE associates with plant membrane nanodomains and binds phosphatidylinositides in vitro
Authors: Xin Xiu-Fang , Kinch Lisa , Cai Boying , Paasch Bradley C. , Kvitko Brian , Grishin Nick V. , He Sheng Yang 
Published: July 2021

ABSTRACTBacterial phytopathogens deliver effector proteins into host cells as key virulence weapons to cause disease. Extensive studies revealed diverse functions and biochemical properties of different effector proteins from pathogens. In this study, we show that the Pseudomonas syringae effector AvrE, the founding member of a broadly conserved and pathologically important bacterial effector family, binds to phosphatidylinositides (PIPs) in vitro and shares some properties with eukaryotic PROPPINs (β-propellers that bind polyphosphoinositides). In planta pull down experiments with transgenic Arabidopsis plants expressing AvrE revealed that AvrE is associated with several plant proteins including plasma membrane lipid-raft proteins. These results shed new light on the properties of a bacterial effector that is crucial for bacterial virulence in plants.
Structure of a germline-like human antibody defines a neutralizing epitope on the SARS-CoV-2 spike NTD
Authors: Altomare Clara G. , Adelsberg Daniel C. , Carreno Juan Manuel , Sapse Iden A. , Amanat Fatima , Ellebedy Ali H. , Simon Viviana , Krammer Florian , Bajic Goran 
Published: July 2021

AbstractStructural characterization of infection- and vaccination-elicited antibodies in complex with antigen provides insight into the evolutionary arms race between the host and the pathogen and informs rational vaccine immunogen design. We isolated a germline-like monoclonal antibody (mAb) from plasmablasts activated upon mRNA vaccination against SARS-CoV-2 and determined its structure in complex with the spike glycoprotein by cryo-EM. We show that the mAb engages a previously uncharacterized neutralizing epitope on the spike N-terminal domain (NTD). The high-resolution structure reveals details of the intermolecular interactions and shows that the mAb inserts its HCDR3 loop into a hydrophobic NTD cavity previously shown to bind a heme metabolite, biliverdin. We demonstrate direct competition with biliverdin and that - because of the conserved nature of the epitope – the mAb maintains binding to viral variants B.1.1.7 and B.1.351. Our study illustrates the feasibility of targeting the NTD to achieve broad neutralization against SARS-CoV-2 variants.
Structural modeling of the hERG potassium channel and associated drug interactions
Authors: Maly Jan , Emigh Aiyana M. , DeMarco Kevin R. , Furutani Kazuharu , Sack Jon T. , Clancy Colleen E. , Vorobyov Igor , Yarov-Yarovoy Vladimir 
Published: July 2021

AbstractThe voltage-gated potassium channel, KV11.1, encoded by the humanEther-à-go-go- Related Gene (hERG) is expressed in cardiac myocytes, where it is crucial for the membrane repolarization of the action potential. Gating of hERG channel is characterized by rapid, voltage-dependent, C-type inactivation, which blocks ion conduction and is suggested to involve constriction of the selectivity filter. Mutations S620T and S641A/T within the selectivity filter region of hERG have been shown to alter the voltage- dependence of channel inactivation. Because hERG channel blockade is implicated in drug-induced arrhythmias associated with both the open and inactivated states, we used Rosetta to simulate effects of hERG S620T and S641A/T mutations to elucidate conformational changes associated with hERG channel inactivation and differences in drug binding between the two states. Rosetta modeling of the S641A fast-inactivating mutation revealed a lateral shift of F627 side chain in the selectivity filter into the central channel axis along the ion conduction pathway and formation of four lateral fenestrations in the pore. Rosetta modeling of the non-inactivating mutations S620T and S641T suggested a potential molecular mechanism preventing F627 side chain from shifting into the ion conduction pathway during the proposed inactivation process. Furthermore, we used Rosetta docking to explore the binding mechanism of highly selective and potent hERG blockers - dofetilide, terfenadine, and E4031. Our structural modeling correlate well with existing experimental evidence involving interactions of these drugs with key hERG residues Y652 and F656 inside the pore and reveal potential ligand binding interactions within fenestration region in an inactivated state.Significance StatementComputational models of hERG potassium channel provide structural insights into an inactivated state and associated drug interactions. Our computational approach will be useful to study ion channel modulation by small molecules.
Recruitment of Scc2/4 to double strand breaks depends on yH2A and DNA end resection
Authors: Scherzer Martin , Giordano Fosco , Ferran Maria Solé , Ström Lena 
Published: July 2021

AbstractHomologous recombination (HR) enables cells to overcome the threat of DNA double strand breaks (DSB), allowing for repair without the loss of genetic information. Central to the HR repair process is the de novo loading of Cohesin around a DSB by its loader complex Scc2/4. Although Cohesin’s accumulation at these sites has been well studied, the prerequisites leading to Scc2/4 recruitment during the repair process are still elusive. Here we investigate which factors are required for recruitment of Scc2 around DSBs in Saccharomyces cerevisiae. To address this question, we combined ChIP-qPCR with a GAL-inducible HO-endonuclease system to generate a site specific DSB in vivo. We find that Scc2 recruitment relies on yH2A and Tel1, but as opposed to Cohesin, not on Mec1. We further demonstrate that binding of Scc2 depends on and coincides with DNA end resection. Although affected by the impact on resection, this recruitment of Scc2 is not directly facilitated by the RSC, SWR1 or INO80 complexes. Our results shed light on the intricate DSB repair cascade leading to the recruitment of Scc2/4 and the subsequent loading of Cohesin.
Construct validation of leaders’ motivating language in the context of the hotel industries of Bangladesh and Malaysia
Authors: Rabiul Md Karim , Fee Yean Tan , Patwary Ataul Karim , Mohamed Ahmad Edwin , Hilman Haim 
Published: July 2021

Purpose
This study aims to validate the motivating language scale developed by Mayfield et al. (1995) in the context of the hotel industries of Bangladesh and Malaysia.

Design/methodology/approach
For Study 1, data were collected from employees (lower and mid-level) of three to five-star hotels in Bangladesh. Customer-contact employees working in four- and five-star hotels in Malaysia participated in Study 2. Both studies featured a cross-sectional survey design.

Findings
Factor analysis revealed that both samples provided three-factor solutions for the motivating language scale in both countries. Convergent, discriminant and nomological validity were assessed by testing with outcome variables of motivating language.

Research limitations/implications
Future studies may use all three versions of the scale (Bengali, Malay and English) to collect data, as all three have been validated.

Originality/value
By validating the Bengali and Malay versions of the motivating language scale, this study contributes to the leadership language literature, specifically that related to hotel industries in developing contexts.
A new operation strategy of PEV parking and hydro storage in smart grid environment
Authors: Nazari Mohammad Esmaeil , Zarrini Farahmand Mahsa 
Published: July 2021

Purpose
The purpose of this study is to solve the optimal operation strategy problem of plug-in electric vehicles (PEV) parking as a demand response (DR) program and hydro storage as an energy storage system in a smart grid environment using a heuristic algorithm.

Design/methodology/approach
Studying the smart grid with DR, renewable energy resources and energy storage systems is necessary. To do this, the heuristic optimization algorithm is developed to solve the scheduling problem. This deterministic algorithm benefits from the definition of appropriate fitness functions.

Findings
For validation, it is shown that reduction of 1.1%–12.5% in pollution and 8.8%–34.8% in total cost are achieved, as compared with literature. Also, the suggested operation strategy of PEVs parking and hydro storage results in reducing the total cost by 6.21%.

Originality/value
DR programs such as PEV parking play a major role in smart grid developments. Also, energy storage systems such as hydro storage lead to better performance of distributed generations and lower costs and pollution by thermal units. However, based on the literature, the effects of PEV parking and hydro storage on smart grid operation strategy are not considered. Therefore, contributions of this study are: effects of hydro storage on the smart grid are considered, effects of PEV parking on the smart grid are considered, a heuristic algorithm is developed to solve operation strategy problem for PEV parking and hydro storage in a smart grid environment.
Health boards' governance of quality and risk: quality improvement agenda for the board
Authors: Avery Mark J. , Cripps Allan W. , Rogers Gary D. 
Published: July 2021

PurposeThis study explores key governance, leadership and management activities that have impact on quality, risk and safety within Australian healthcare organisations.Design/methodology/approachCurrent non-executive directors (n = 12) of public and private health boards were interviewed about contemporary approaches to fiduciary and corporate responsibilities for quality assurance and improvement outcomes in the context of risk and safety management for patient care. Verbatim transcripts were subjected to thematic analysis triangulated with Leximancer-based text mining.FindingsBoards operate in a strong legislative, healthcare standards and normative environment of quality and risk management. Support and influence that create a positive quality and risk management culture within the organisation, actions that disseminate quality and risk broadly and at depth for all levels, and implementation and sustained development of quality and risk systems that report on and contain risk were critical tasks for boards and their directors.Practical implicationsFindings from this study may provide health directors with key quality and risk management agenda points to expand or deepen the impact of governance around health facilities' quality and risk management.Originality/valueThis study has identified key governance activities and responsibilities where boards demonstrate that they add value in terms of potential improvement to hospital and health service quality care outcomes. The demonstrable influence identified makes an important contribution to our understanding of healthcare governance.
Fostering healthcare innovation: a qualitative study of dental implant product development by Thai university researchers
Authors: Sooampon Sutti , Pisarnturakit Pagaporn Pantuwadee , Sooampon Sireerat 
Published: July 2021

PurposeThis study investigated the conditions required to foster healthcare innovation. Due to the limited research and development (R&D) capability of the Thai private sector, universities are increasingly expected to be emerging sources of healthcare products. However, the lack of experience in research commercialization requires that whether and how Thai university researchers can serve this mission be explored.Design/methodology/approachA promising dental implant product development project was investigated using the single-case research methodology. In-depth interviews were conducted with three key members involved in this innovative project. Questions concerning how a team of university researchers could embark on and overcome the obstacles encountered during their entrepreneurial project were asked. Based on the conceptual foundation of academic entrepreneurship, primary and additional secondary data were analyzed to acquire knowledge of academic entrepreneurship in healthcare in an emerging economy.FindingsHealthcare inequality has generated entrepreneurial initiatives by healthcare researchers to develop low-cost dental implants. However, their efforts have been insufficient to progress to the commercialization stage. An informal relationship among socially oriented partners, including interdisciplinary experts, not-for-profit-oriented manufacturers and early adopters contributed to this project's success.Originality/valueTo foster healthcare innovation, the authors’ micro-level evidence of a socially oriented partnership on an informal basis indicates the need for inter-professional governance that maintains a cross-organizational environment between healthcare researchers and external parties. The future contributions to innovation management by healthcare professionals should be enhanced. A socially entrepreneurial model of healthcare innovation is suggested for further consideration as an enabling policy option for inter-professional partnership in emerging economies.
Influence of selection criteria on clients’ satisfaction with construction consultancy services in Nigeria
Authors: Ogbu Chukwuemeka Patrick , Imafidon Monday Omogiate 
Published: July 2021

Purpose
To receive a satisfactory consultancy service, a construction client must first select suitable consultants. While numerous criteria for the selection of construction consultants have been suggested in the literature, their influence on client satisfaction has hardly been statistically established. This study aimed to reduce the criteria for the selection of construction consultants into a more manageable set of fewer factors and ascertain the influence of the factors on client satisfaction.

Design/methodology/approach
Data were gathered through a purposively administered cross-sectional survey of public sector clients in Nigeria. Analyzes were done using relative importance index, factor analysis and multiple regression analysis.

Findings
The results proved that the criteria for the selection of consultants can, in the order of decreasing influence on client satisfaction, be grouped into service delivery approach (SDA), relationship with the client, the caliber of personnel, firm reputation and firm certification. However, SDA is more influential on client satisfaction. All the factors were found to have significant statistical effects on clients’ overall satisfaction with consultancy services.

Originality/value
The study demonstrates the extent to which construction consultant selection criteria are relevant to client satisfaction. It shows that SDA is the most important predictor of clients’ satisfaction with consultancy services. The results are helpful for grouping consultant selection criteria in future studies, and in guiding clients on the weights to assign to consultant selection criteria during tender evaluation.
Access to non-violent pornography in a secure forensic hospital
Authors: Nally Thomas , Ireland Jane L. , McNeill Kimberley , Birch Philip , Ireland Carol A. 
Published: July 2021

Purpose
The purpose of this paper is to explore non-violent pornography within secure hospital settings.

Design/methodology/approach
It includes a systematic review (n = 40 papers), followed by a qualitative study comprising semi-structured interviews (n = 24, 6 patients and 18 staff) and staff focus groups (n = 22 staff).

Findings
The systematic review identified six themes, as follows: pornography is inconsistently defined, pornography exposure can increase general aggression, pornography exposure may increase the risk for sexual aggression, pornography exposure can increase aggression supportive beliefs, pornography exposure impacts negatively on those with a violent predisposition and pornography is educational for men not identifying as heterosexual. The semi-structured interviews and focus groups revealed four themes as follows: staff members hold diverse beliefs about pornographic material, pornography is difficult to obtain and use for patients who do not identify as heterosexual, pornography is used for specific functions, and frequent exposure to pornography can have negative effects for staff members.

Practical implications
Trying to obtain consensus on the impacts of pornography on forensic patients is not possible; material access decisions should be on a case-by-case basis. Policy decisions should be based on fully represented views, including the LGBTI community. The impacts on staff of their occupational exposure to such material should be recognised and support provided. Clinical decision-making in this area should consider not only if access should be allowed but also how it can be managed safely, if at all. This includes for all those who could be exposed to such material, inadvertently or otherwise.

Originality/value
It addresses the under-researched area of patient access to pornography, capturing a poorly considered sample, namely, high secure psychiatric.
A review of property rights in outer space from a land management perspective
Authors: de Vries Walter Timo , Hugentobler Urs 
Published: July 2021

Purpose
In light of the discussions on outer space property management, this conceptual review paper aims to discuss and evaluate if, when and under which conditions certain land management and property right frameworks can apply to allocate and/or restrict property rights in outer space.

Design/methodology/approach
This paper applies a pragmatic review approach which seeks to better understand if and how the basic tenets of the land management frameworks could better shape and revise the challenges in outer space regulations.

Findings
Despite the fact that regulatory guidelines on outer space rights are existing, the analysis shows that these lack a number of practical tools and measures aiming at intervening if stakeholders do not follow the rules. With the use of land management frameworks, it is possible to derive policy options for making the outer space management more practical and action-oriented, in particular for the removal of space debris. These include amongst others more attention for formulating global public restrictions in outer space, incorporating regulatory guidelines for accessing open space regimes, addressing responsiveness and robustness in adherence and compliance to regulations

Research limitations/implications
Given the conceptual and discursive character of the paper, there are no specific empirical data, yet several recommendations for further research include expanding the boundary work between the land management and regulatory outer space domain.

Practical implications
The insights derived from land management and real estate related property theories could potentially provide new starting points for (re)formulating the regulatory framework for outer space property discourses.

Social implications
Interpreting the outer space regulations from known and practiced land management perspective helps to bridge the policy–society knowledge and necessity gap on outer space activities.

Originality/value
The specific land management perspective and discursive analysis on outer space debris provide new options for devising and extending regulatory guidelines for assigning responsibilities on outer space debris and debris rights, restrictions and responsibilities.
Customer experience research: intellectual structure and future research opportunities
Authors: Silva Jorge H.O. , Mendes Glauco H.S. , Cauchick Miguel Paulo A. , Amorim Marlene , Teixeira Jorge Grenha 
Published: July 2021

PurposeThis article aims to synthesize and integrate current research on customer experience (CX), identifying the intellectual structure of the field, systematizing a conceptual framework and identifying future research opportunities.Design/methodology/approachTo analyze 629 articles published in peer-reviewed journals in almost four decades, this study employs both bibliometric co-keyword and thematic literature analysis in a complementary way.FindingsThis article maps the CX literature by describing its intellectual structure in terms of three research domains (customer, organizational and technological), their corresponding most relevant research themes and topics. Moreover, this study develops a conceptual framework and research propositions to summarize and integrate the CX literature. This work recognizes technology as an important driver for the development of CX research. Lastly, this article provides future research opportunities for moving the field forward, considering an integrative view among domains.Originality/valueThis paper complements other reviews on CX by using a novel methodological approach (co-keyword and thematic analysis) that enables the identification and visualization of the CX intellectual structure. In addition, the study explores the increasing connection between technology and CX research, by raising evidence that technology, by continuously modifying services and consequently CX, has become a transversal component in the research field. These outcomes may be useful for academics and practitioners.
Managing material value creation matters in integrated reporting
Authors: Steenkamp Natasja , Roberts Roslyn 
Published: July 2021

Purpose
This paper aims to explore how advanced integrated report preparers internalise and operationalise material value creation information to manage the generation of such information for the integrated report.

Design/methodology/approach
The paper adopts a qualitative approach using in-depth semi-structured interviews to examine how information about material value creation matters in six South African organisations are managed.

Findings
The findings will be useful to integrated reporting adopters as to how they might implement appropriate processes and systems to determine, communicate, collect and process information about matters that substantively affect their value creation.

Originality/value
The paper contributes to the body of knowledge by providing insight on how material value creation matters are determined, communicated internally and information about such matters generated.
Effect of silicone based softener on fabric handle properties made by ring, rotor and MVS yarns
Authors: Anam Wardah , Akhtar Khurram Shehzad , Ahmad Faheem , Rasheed Abher , Rasheed Abher , Mohsin Muhammad , Azam Farooq , Ullah Tehseen , Ahmad Sheraz 
Published: July 2021

Purpose
The purpose of this study was to produce yarns from three different spinning techniques, i.e.Murata Vortex Spinning (MVS) ring spinning and rotor spinning. Those yarns were then used to produce fabrics. Then, the effect of silicone softener on tactile comfort of fabric was investigated.

Design/methodology/approach
Three different yarns, i.e. Ring, Rotor and MVS yarns, were used to make fabrics using CCI sample loom which were then subjected to post treatments like desizing, scouring and bleaching. After the completion of the dyeing process, silicone-based softener was used to improve the hand feel of fabrics. The structures of three yarns were evaluated using Scanning electron microscopy. The fabrics were evaluated against compression, bending and surface properties using Kawabata evaluation system.

Findings
The fabric made of MVS yarn depicted more geometrical roughness, coefficient of friction and bending rigidity but less compressibility as compared to fabrics made with other yarns. It was observed that softener concentration has a direct relationship with thickness and bending rigidity of the fabric, and inverse relationship with coefficient of friction and geometrical roughness of the fabric.

Originality/value
MVS yarn has some superior properties over rotor and ring spun yarn like high production rates, high resistance to pilling, clear appearance and stability against deformation but has disadvantage that it has less compressibility. Therefore, softener is applied on the fabric, to address this issue, so that it could also be used for apparels application.