bluuebunny/crossref_metadata_2025_split huggingface dataset:
Molecular Signature of Subtypes of Renal cell carcinomas and Immunotherapy strategy
Authors: Guo Jianing , Lv XiaoFei , He Bin , Wang Pu , Liu Shenglai , Jiang Ning 
Published: September 2021

Abstract
BackgroundRenal cell carcinoma (RCC) was not a single disease, many efforts have been devoted to identifying RCC subtypes on the basis of genomic profiling, but none has classified immunogenomic profiling based on therapeutic responses in RCC subtypes.MethodsTumors from RCC patients from The Cancer Genome Atlas (TCGA) cohort were analyzed, and genomic profiling was performed. We classified RCC on the basis of the immunogenomic profiling of 29 immune signatures.ResultsWe investigated the transcriptional changes of three RCC subtypes by RNA-seq. Gene ontology (GO) identify specific gene signatures differed significantly between KIRC, KIRP and KICH related to the distinct pathways. Site of origin within the nephron was one major determinant in the molecular and immune classification, reflecting differences between three subtypes. The Immunity High KIRC and KIRP subtype was enriched not only in immune signatures, but also including PD-L1 expression signaling, NF-kappa B signaling pathway, JAK-STAT signaling pathway and Cell cycle signaling pathway. KICH was a distinct disease that shared little genomic characteristics with KIRC and KIRP.ConclusionsThe identification of RCC subtypes based on immune signatures has potential clinical implications for RCC treatment. It is imaginable that patients with higher immunity subtype of KIRC and KIRP would be more likely to respond to anti-PD-1/ PD-L1 therapy than patients with KICH subtype. CCL21 and CCL25 might be a potential target for KICH therapy.
Successful Resumption of Football Competition With Spectators During the Active Phase of COVID-19 Pandemic: Experiences From A Rapidly Developing Country
Authors: Khan Naushad , Musleh AbdulWahab Al , Abdurahiman Sameer , Asim Mohammad , El-Menyar Ayman , Al-Thani Hassan 
Published: September 2021

Abstract
Background: COVID-19 has interrupted professional sporting activities for more than a year and sent shockwaves across the society. Objectives: To evaluate the resumption of professional football event with fans and the likely spread of COVID-19 infections. Design: A retrospective observational studyMethods: The study involved football players, match officials, local organizing committee (LOC) members, working in close coordination, and over 16,000 spectators in the state of Qatar. We examined data from the Amir Cup final (December 18th , 2020), which was played under a strict protocol that included extensive SARS-CoV-2 RT-PCR testing for players and match officials, as well as the utility of COVID-19 rapid antigen testing and antibody testing as a tool for screening spectators to ensure their safe return to the stadiums. In addition, we reviewed the guidelines and protocols that were put in place to organize Qatar's Amir Cup Football Final, which drew over 16,000 fans in the stadium.Results: A total of 16171 spectators undertook rapid antigen and antibody tests for the Amir cup final (From Dec16-Dec18, 2020). Fifteen Spectators (n=15) returned with a positive result for COVID-19 infection during the final event (Positivity rate =0.12%). All players underwent RT-PCR testing 48 hours before the match. None of the players tested positive for Covid-19 infections. 1311 individuals reported having symptoms related to COVID-19 post final of Amir Cup. These spectators were tested for COVID-19 RT-PCR with an overall positivity rate (positive/reactive) to be 0.42% (69/16171).Conclusion: This report shows a meagre incidence rate of COVID-19 infections during and post-Amir Cup final, and based on this, it appears that during the ongoing COVID-19 pandemic, the supervised and controlled resumption of football matches with spectators can be carried out safely following a strict testing and tracing protocol.
Drone Surveys Revealed Bottom-Up, And Not Top-Down, Effects On The Marsh Deer Local Abundance
Authors: Brack Ismael Verrastro , Kindel Andreas , Berto Douglas Oliveira , Cordeiro José Luis Passos , Coelho Igor Pfeifer , Lahoz-Monfort José Joaquín , Oliveira Luiz Flamarion Barbosa 
Published: September 2021

Abstract
Context: Spatial variation in large herbivore populations can be highly affected by the availability of resources (bottom-up) but modulated by the presence of predators (top-down). Studying the relative influence of these forces has been a major topic of interest in ecological and conservation research, while it has also been challenging to sample large herbivores. Objective: i) Explore the use of spatiotemporally replicated drone-based counts analysed with N-mixture models to estimate abundance of large herbivores. ii) Evaluate the relative influence of bottom-up (forage and water) and top-down (jaguars) processes on the local abundance of the threatened marsh deer.Methods: We conducted spatiotemporally replicated drone flights in the dry season of Pantanal wetland (Brazil) and imagery was reviewed by either one or two observers. We fitted counts using N-mixture models (for single and double observer protocols) and modelled local abundance in relation to vegetation greenness, distance to water bodies, and jaguar density.Results: We found a positive relationship of marsh deer local abundance with vegetation greenness, a negative relationship with distance to water, but no relation with jaguar density. Individuals were concentrated in the lower and wetter region, even though it is the area expected to be more lethal from jaguar predation.Conclusions: Bottom-up processes are shaping the distribution of marsh deer in the dry season; the benefits of accessing high-quality areas outweigh predation risk from jaguars. Spatiotemporally replicated drone-based counts may serve as an accessible and cost-effective protocol for large herbivores abundance estimation and monitoring while accounting for imperfect detection.
Structural Damage Identification Method Based On Transmissibility in Time Domain
Authors: Zou Yunfeng , Lu Xuandong , Yang Jinsong , He Xuhui , Wang Tiantian 
Published: September 2021

Abstract
Structural damage identification technology is of great significance to improve the reliability and safety of civil structures and has attracted much attention in the study of structural health monitoring. In this paper, a novelty structural damage identification method based on the transmissibility in time domain is proposed. The method takes the discrepancy of transmissibility of structure response in time domain before and after damage as the basis of finite element model modification. The damage location and damage degree are obtained through iteration by minimizing the difference between the measurements at gauge locations and the reconstruction response extrapolated by FE model. Taking the advantage of the response reconstruction method based on empirical mode decomposition, the damage information is possible to obtain in the absence of prior knowledge on external excitation information. Moreover, this method is carried out in the time domain, without the need to identify the modal parameters and perform time-frequency analysis, which simplicity ensures the high efficiency of damage identification. The effectiveness and accuracy of the proposed method are studied by simulation, including reconstruction error and measurement noise. The identification results demonstrate that the proposed structural damage identification method improves the calculation effectiveness considerably and ensures the identification accuracy.
The efficacy of bariticinib in real-world patients with refractory or severe juvenile dermatomyositis:a monocentric retrospective study
Authors: Wang Zhaoling , Zheng Qi , Xu XiSheng , Lu MeiPing 
Published: September 2021

Abstract
Objective

To evaluate the efficacy and safety of low dose baricitinib in children with refractory or severe juvenile dermatomyositis(JDM) in a real-world setting.
Methods

A monocentric retrospective real-world study was conducted, in which fourteen refractory and one severe newly diagnosed JDM patients were included. These patients were all treated by low dose baricitinib (below the recommended dose) combined with corticosteroids and or immunosuppressive agents. Clinical data were collected at the baseline and 4, 12, 24 weeks after baricitinib implication. Treatment response (complete response, CR, Partial response, PR and non-response,NR) was evaluated using both the Paediatric Rheumatology International Trials Organization (PRINTO) remission criteria and skin Disease Activity Score (DAS). All the adverse events (AEs) were recorded.
Results

After baricitinib treatment, all 15 patients showed improvement of skin involvement, including 14 patients with recurrent skin rashes and one newly diagnosed JDM. Calcinosis stabilized in two patients (2/3) and partially regressed in one. Four patients (4/15) had interstitial lung disease (ILD), which normalized in one, improved in two and stabilized in one. One patient complicated with macrophage activation syndrome (MAS) achieved clinical remission. CR was achieved in 3/15 patients, ranging from 4 to 12 weeks after baricitinib initiation. Five patients (5/15) got PR 4 to 24 weeks after baricitinib use. Daily steroid dosage was decreased from 0.632 mg/kg to 0.357 mg/kg (P = 0.043) at 24 weeks in all responders. However, there was no statistically difference in muscle improvement. One patient was stopped using baricitinib because of varicella zoster virus infection, while no other serious side effect was observed in this study.
Conclusion

Low dose baricitinib had efficacy and was safe to applied in refractory or severe JDM patients, especially for recurrent skin rashes. Baricitinib may also be helpful for JDM complicated with ILD and MAS.
Temperature Environmental Adaptability Research of HNIW/FOX-7 Based PBXs
Authors: Lan Guanchao , Chao Hui , Wang Na , Yan Bo , Li Xiao , Jin Shaohua 
Published: September 2021

Abstract
In this study, HNIW/FOX-7 based PBX modeling powders and PBX columns were treated by LT (low temperature), HT (high temperature), HLC (high-low temperature cycle) and HLS (high-low temperature shock) to study temperature environmental adaptability of HNIW/FOX-7 based PBXs. Then SEM, IR, XRD and DSC were used to study the variation of PBX modeling powders after LT, HT, HLC and HLS treatments; in addition, the mass, size and mechanical properties of PBX columns were characterized after different temperature adaptability treatments as well. The results indicate that the change ratios of mass and size of HNIW/FOX-7 based PBX columns are less than 1%, illustrating that mass and size of PBX columns are at acceptable level after different temperature adaptability treatments. The unevenness degree of the surface of PBX modeling powders followed the order of HLC>HT>LT>HLS, which agrees well with mass loss order. Moreover, IR and XRD results indicated that the molecular structure and crystal form of HNIW and FOX-7 did not change after different temperature adaptability treatments. Additionally, thermal stabilities of PBX modeling powders are decreased after different temperature adaptability treatments, among which HLS has the largest influence on HNIW/FOX-based PBX modeling powders. The compression strengths and elastic moduli of HNIW/FOX-based PBX columns are enhanced after different temperature adaptability treatments, among which the strength of PBX columns after HLC has the maximum increase, indicating that HLC has more significant effect on mechanical property.
Minimal mechanistic component of proteasome activation and prevention of impairment by pathological oligomers
Authors: Chuah Janelle , Thibaudeau Tifffany , Smith David 
Published: September 2021

Abstract
Impairment of proteasomal function has been implicated in neurodegenerative diseases, justifying the need to understand how the proteasome is activated for protein degradation. Here, using biochemical and structural (cryo-EM) strategies in both archaeal and mammalian proteasomes, we further determine the HbYX(-motif)-dependent mechanism of proteasomal activation used by multiple proteasome-activating complexes including the 19S Particle. We identify multiple proteasome α subunit residues involved in HbYX-dependent activation, a point mutation that activates the proteasome by partially mimicking a HbYX-bound state, and conformational changes involved in gate-opening with a 2.0A structure. Through an iterative process of peptide synthesis, we successfully design a HbYX-like dipeptide mimetic as a robust tool to elucidate how the motif autonomously activates the proteasome. The mimetic induces near complete gate-opening at saturating concentration, activating mammalian proteasomal degradation of peptides and proteins. Findings using our peptide mimetic suggest the HbYX-dependent mechanism requires cooperative binding in at least two intersubunit pockets of the α ring. Collectively, the results presented here unambiguously demonstrate the lone role of the HbYX tyrosine in the allosteric mechanism of proteasome activation and offer proof of concept for the robust potential of HbYX-like small molecules to activate the proteasome.
Myocarditis Incidence, Complications and Mortality in Sweden: A Retrospective Registry Study From 2000-2014
Authors: Fu Michael , Kontogeorgos Silvana , Thunström Erik , Sandström Tatiana Zverkova , Kroon Christian , Bollano Entela , Schaufelberger Maria , Rosengren Annika 
Published: September 2021

Abstract
Objectives: Investigate trends in incidence and prognosis of myocarditis in Sweden during 2000-2014.Background: Myocarditis is an inflammatory heart disease, with scarce data concerning incidence and prognosis.Methods: Linking Swedish National Patient and Cause of Death Register, we identified individuals ≥16 years with first-time diagnosis of myocarditis during 2000-2014. Reference population, matched for age and birth year (n=16 622) was selected from the Swedish Total Population Register. Results: Among the 8 679 cases, (75% men, 64% <50 years), incidence rate/100 000 inhabitants rose from 6.3 to 8.6, mostly in men and those <50 years. Incident heart failure/dilated cardiomyopathy occurred in 6.2% within 1 year after index hospitalization and in 10.2% during 2000-2014, predominantly in those ≥50 years (12.1% within 1 year, 20.8% during 2000-2014). In all, 8.1% died within 1 year, 0.9% (<50 years) and 20.8% (≥50 years). Hazard ratios (adjusted for age, sex) for 1-year mortality comparing cases and controls were 4.00 (95% confidence interval 1.37-11.70), 4.48 (2.57-7.82), 4.57 (3.31-6.31) and 3.93 (3.39-4.57) for individuals aged <30, 30-<50, 50-<70, and ≥70 years, respectively. Conclusion: The incidence of myocarditis during 2000-2014 increased, predominantly in younger men. One-year mortality in the young was low, but fourfold higher compared with reference population.
Activation of Alpha7 Nicotinic Acetylcholine Receptors Reduce Chronic Pain-induced Anxiety and Depression-Like Behaviors via WNT/β-Catenin Pathway
Authors: Gong Xingrui , Chen Yongmei , Fan Rongmei , Cai Meihua , Zhang Mazhong 
Published: September 2021

Abstract
Chronic pain frequently leads to anxiety, depression and microglia-related inflammation is the common mechanism for chronic pain and psychiatric disorders. Activation of alpha 7 nicotinic acetylcholine (α7 nAch) receptor suppresses microglia-related inflammation. Thus, we evaluated the activation of α7 nAch receptors on chronic pain-induced anxiety- and depression-like behaviors and explored the potential mechanisms. Open field, sucrose preference, and pain behavior tests (paw withdrawal threshold, and cold response to acetone) were evaluated in male Wistar rats that received spared nerve injury (SNI) and complete Freund’s adjuvant (CFA) into the unilateral ankle articular cavity. The effect of intracerebroventricular (ICV) injection of α7 nAch receptor agonist PHA and α7 nAch receptor antagonist on anxiety and depression-like behaviors and cytokines expression were examined. Cytokines in medial prefrontal cortex (mPFC), basolateral amygdala (BA), and ventral hippocampus (VH) were measured using enzyme-linked immune absorbent assay. SNI surgery and CFA injection induced anxiety- and depression-like behaviors, and ICV injection of PHA reduced that anxiety- and depression-like behaviors. SNI surgery and CFA injection skewed the mPFC, BA, and VH to a pro-inflammatory response and ICV injection of PHA shift brain to an anti-inflammatory response in those sites. The anti-depressive effect and anti-inflammatory effect of PHA were reversed by WNT/β-catenin inhibitor. Activation of alpha7 nicotinic acetylcholine receptors reduces chronic pain-induced anxiety- and depression-like behaviors, the mechanisms may be attributed to the activation of WNT/β-catenin pathway and suppression of brain inflammation.
Comparison of Changes in Lipid Profiles of Eastern Chinese Premenopausal Women with Early-Stage Breast Cancer Treated with Different Endocrine Therapy: A Retrospective Study
Authors: Wang Kaiyue , Shen Lu , Wu Yinan , Zhang Suzhan , Tian Wei 
Published: September 2021

Abstract
Background: Adjuvant endocrine therapy improves the prognosis of early breast cancer with hormone receptor positivity. However, there is no systematic report on the effect of endocrine therapy (especially ovarian function suppression, OFS) on serum lipids in premenopausal women. This retrospective cohort study aimed to determine whether various endocrine treatments had different effects on blood lipids in young premenopausal breast cancer patients. Methods: This study enrolled 160 premenopausal patients with stage I-III breast cancer in eastern China. The initial diagnostic information was retrieved from patient's medical records, including age at the time of diagnosis, tumor characteristics, anticancer treatment, weight and height, and past medical history. They have no history of cardiovascular disease. The changes of blood lipids in the types of endocrine therapy were compared at the 3rd, 6th, 12th, and 24th months after the start of endocrine therapy. Generalized Linear Mixed Model (GLMM) was used in analyses Results: Our data revealed that LDL-C of patients with TAM group in the 6th, 12th, and 24th months was significantly lower than that in the 3rd month, while HDL-C in the 6th, 12th, and 24th months was significantly higher than that in the 3rd month, indicating that blood lipid levels generally improved with time. While in TAM plus OFS group, HDL-C in the 24th month was significantly higher than that in the 3rd month, TC in the 24th month was significantly higher than that in the 6th month. The lipid profiles of OFS plus AI group did not show significant differences at any time point but were significantly higher than those of the other two groups.Conclusions: TAM group tended to have lower serum lipid levels. With longer follow-up, no statistically significant difference in values at various time points was observed between TAM and TAM plus OFS groups. Compared with the other two groups, OFS plus AI group presented an increasing trend toward LDL-C and TC. The risk of dyslipidemia requires further investigation using a large sample size.
Acute Severe Asthma: Changes in Patient Characteristics, Management, and Outcomes over a Period of 20 Years (1997 to 2017), Insights from Cub-Réa Network.
Authors: Younan Romy , Augy Jean Loup , Hermann Bertrand , Guidet Bertrand , Aegerter Philippe , Guerot Emmanuel , Novara Ana , Hauw-Berlemont Caroline , Hamdan Amer , Bailleul Clotilde , Santi Francesca , Diehl Jean-Luc , Peron Nicolas , Aissaoui Nadia 
Published: September 2021

Abstract
Background: While acute severe asthma (ASA) is the leading cause of emergency department visits and the third cause of hospitalization in children younger than 18 years old, there is a lack of data regarding adult patients admitted in intensive care units (ICU) for ASA. We aimed to describe the evolutions in epidemiology, management, and outcomes of ASA in adult patients, over a period of twenty years in the Greater Paris area ICUs (CUB-Réa Database). Methods: Demographics, severity and supportive treatments were collected from the CUB-Réa Database. The primary endpoint was the prevalence of ASA by periods of 5 years. The secondary endpoints were in-ICU survival, in-hospital survival, use of mechanical ventilation including non-invasive and invasive and catecholamine. Multivariate analysis was performed to assess correlating factors of ICU Mortality. Results: Of the 475 357 ICU admissions from January 1997 to January 2016, 7049 were admitted for ASA with a decreasing prevalence over time, respectively 2.8%, 1.7%, 1.1%, and 1.1% of total ICU admissions (p <0.001). The median age was 46 years old [IQR: 25%-75%: 32-59], 3906 (55%) were female, the median SAPS II was 20 [IQR: 13-28], and 1501 (21%) had mechanical ventilation. Over time, age, the SAPSII and the Charlson Comorbidity Index tended to increase. The use of invasive and non-invasive mechanical ventilation increased (p < 0.001), whereas the use of catecholamine decreased (p <0.001). The in-ICU survival rate improved from 97% to 99% (p=0.008). In the multivariate analysis, factors associated with in-ICU mortality were SAPSII (p < 0.001), renal replacement therapy (p < 0.001), catecholamine (p < 0.001), cardiac arrest (p < 0.001), pneumothorax (p < 0.001), ARDS (p < 0.001), sepsis (p < 0.001) and IMV (p < 0.001). Conclusion: ICU admission for ASA remains uncommon and decreases over time. Despite an increasing severity of patients and the use of mechanical ventilation, the use of catecholamine decreases with high in-ICU survival rate which could be related to a better management of mechanical ventilation.