bluuebunny/crossref_metadata_2025_split huggingface dataset:
Rhizo-inoculation of phosphate solubilizing bacteria strains to improve rice (Oryza sativa L. var. FARO 44) growth under ferruginous ultisol conditions.
Authors: Ibrahim Musa Saheed , Ikhajiagbe Beckley 
Published: November 2021

Abstract
Background
The research investigated the possibility of phosphate solubilizing bacteria (PSB) with plant growth-promoting (PGP) capabilities to improve growth properties of rice plant under ferruginous ultisol (FU) condition through rhizo-inoculation strategy. The PSB with PGP properties used in this research were Bacillus cereus strain GGBSU-1, Proteus mirabilis strain TL14-1 and Klebsiella variicola strain AUH-KAM-9 that were previously isolated and characterized following the 16S rRNA gene sequencing. The rice seeds were sown in a composite FU soil sample and a humus soil (control) and then rhizo-inoculated along the root region of the growing rice seedling at 16 days after sowing. The rice plant was studied for differences in morphological, physiological and biomass parameters for 16 weeks after rhizo-inoculation.
Results
The composite FU soil used in the study had high pH, low bioavailable phosphorus, low water holding capacity and high iron levels which has led to a low growth properties of rice seeds sown in FU soil without rhizo-inoculation. After rhizo-inoculation, a significant increase in morphological, biomass and physiological parameters were observed in the rice plant grown in the FU soil as against the control and the rice plant in FU soil without inoculation except for terpenoid which is usually known to signify biotic stress and as part of plant defense mechanism. Generally, Bacillus cereus strain GGBSU-1 showed higher improvement compared to other PSBs used in the present study. This is due to a more improved growth properties observed.
Conclusion
Rhizo-inoculation of rice seedling with Bacillus cereus strain GGBSU-1, Proteus mirabilis strain TL14-1 and Klebsiella variicola strain AUH-KAM-9 under FU soil condition significantly improved growth properties of the rice plant. This suggest the ability of PSB to solubilize and mineralize soil phosphate and improve its availability for plant use in phosphate stressed soil, thereby improving plant growth properties.
The Effects of Vegetation Restoration and Season On Soil Enzyme Activity and Microbial Communities in Karst Rocky Desertification Area
Authors: Guo Zhang , Zheng Chunyan , Chen Zhu , Wang Jin , Yu Yanghua , Liu Ziqi , Yang Guangbin , Xiong Kangning 
Published: November 2021

Abstract
Aims The process of karst rocky desertification has been closely related to improper land use in southwest China. Now this habitat is the subject of an important ecological restoration project. However, the changes in soil properties and microbial characteristics in response to this vegetation restoration remain poorly understood.Methods We investigated four vegetation types, including dragon fruit, Chinese pepper, walnut teak, with corn as a control, in southwest China, in 2019. We measured the impacts of these vegetation types on soil properties and microbial biomass, enzyme activity, and microbial community composition (using high-throughput sequencing technology).Results The different vegetation types had significantly different impacts on soil exchangeable Ca2+, soil organic carbon and available nutrients. The vegetation types also significantly affected microbial biomass. Soil enzyme activity, including b-1,4-glucosidase, b-1,4-N-acetylglucosaminidase, alkaline phosphatase, and catalase, were significantly different among vegetation types. All vegetation types were dominated by the bacterial phyla Acidobacteria, Proteobacteria, and Actinobacteria and the fungal phylum Ascomycota, except for corn which was dominated by the fungal phylum Mucoromycota. Non-metric multidimensional scaling (NMDS) showed that the vegetation type exhibited different microbial b-diversity, especially in winter. The vegetation type, season, and soil properties collectively explained 46% and 59% of soil bacterial and fungal community composition, respectively. The bacterial-fungal interactions under the six vegetation types were distinctly different between summer and winter.Conclusions Compared with traditional corn, the restoration of natural vegetation partially reversed KRD by improving soil properties, increasing microbial biomass, and differentiating the microbial community structures in the different vegetation types.
Nitrogen Application Increases Soil Organic Carbon And Crop Productivity By Altering Soil Autotrophic Bacterial Community On the Semiarid Loess Plateau
Authors: Wang Jinbin , Xie Junhong , Li Lingling , Luo Zhuzhu , Zhang Renzhi , Jiang Yuji 
Published: November 2021

Abstract
Background and aims Soil autotrophic microorganisms play crucial roles in carbon fixation and crop productivity. However, the information is remains limited to whether and how soil autotrophic microbe mediate SOC pool dynamics and crop productivity.Methods Here, we investigated the effects of the structure and co-occurrence network of soil autotrophic bacterial community on SOC storage and maize yield. A long-term field experiment was conducted with four chemical nitrogen (N) application rates on the semi-arid Loess Plateau, including N application at 0 kg ha–1 (N0), 100 kg ha−1 (N1), 200 kg ha−1 (N2), and 300 kg ha−1 (N3), respectively. Results Our results showed that the N application significantly impacted the structure and co-occurrence network of soil cbbL-carrying bacterial community via changing soil pH, nitrate nitrogen (NO3-N), and soil water content (SWC). The diversity of soil autotrophic bacterial community decreased with the increasing rate of N application. We detected a high abundance of the autotrophic bacterial dominant genera Xanthobacter, Bradyrhizobium, Aminobacter, and Nitrosospira. The co-occurrence network of autotrophic bacteria contained four distinct modules that consisted of closely interconnected microorganisms. Structural equation modeling further indicated that the diversity, composition, and network of autotrophic bacterial community had significant relationships with SOC storage and maize yield.Conclusion Taken together, our results highlight that the soil autotrophic bacterial community may drive carbon fixation SOC accumulation and contribute positively to crop productivity.
The correlation of fibrinogen-like protein-1 expression with the progression and prognosis of hepatocellular carcinoma
Authors: Hua Nanni , Yang Chen , Chen Anxian , Feng Yi , He Xianglei , Ru Guoqing , Tong Xiangmin , Wang Shibing 
Published: November 2021

Abstract
Background

ConclusionsFibrinogen-like protein-1 (FGL1), as the member of FREP superfamily, had been widely concerned as a major immune inhibitory ligand of LAG-3. Although FGL1 expression levels didn’t have significant difference in most of tumors via online data analysis, we found that it was down-regulated in liver cancer. Moreover, the correlation between FGL1 expression and the progression and prognosis of hepatocellular carcinoma (HCC) was still disputed.
Methods

In our study, firstly, we used bioinformatics analysis to define the expression profile and clinical significance of FGL1 in HCC. Then, we determined the FGL1 level in human HCC cell lines, tumor and normal liver tissues from HCC patients by western blot. Furthermore, tissue microassays were used to detect the expression of FGL1 through immunohistochemistry staining and verify whether FGL1 expression levels were associated with clinicopathological features of HCC patients.
Results

The results proved that FGL1 was down-regulated significantly in HCC cell lines and HCC tissues, corresponding with the results of our bioinformatics analysis. FGL1 expression levels in HCC were related to Edmondson grade and metastasis. Additionally, high FGL1 expression was related to better overall survival in HCC patients, indicating that the down-regulated FGL1 was correlated with poor prognosis and FGL1 might function as a tumor suppressor.
Conclusions

Taken together, expression levels of FGL1 may correlate with the progression and prognosis of HCC, and FGL1 could be a potential prognostic biomarker.
Predictive Value of the PaO2/FIO2 Ratio for Mortality in Patients with Acute Respiratory Distress Syndrome: A Systematic Review and Meta-Analysis
Authors: Yoshimura Satoshi , Hashimoto Katsuhiko , Shono Yuji , Tamura Takahiro , Uchimido Ryo , Ando Koichi , Okamori Satoshi , Yoshida Takuo , Yoshitake Shigenori , Okada Yohei 
Published: November 2021

Abstract
BackgroundPaO2/FIO2 (P/F) ratio has been used to define the severity of acute respiratory distress syndrome (ARDS) despite the controversy of its clinical utility. This systematic review and meta-analysis (SRMA) aimed to obtain summary estimates of predictive performance of the P/F ratio for predicting mortality in ARDS patients.MethodsWe included a study wherein the study population comprised ARDS patients in anyclinical setting. Medline and Cochrane Central Registry of Controlled Trials were searched for all English language articles. We performed a SRMA on the accuracy of diagnostic prognostic tests using QUADAS-2 tool to evaluate the risk of bias. To pool the results, we applied the bivariate model and obtained summary point estimates of sensitivity and specificity with 95% CIs.ResultsTwenty-eight trials and 38270 patients were included in the meta-analysis. Most of the study settings were in the intensive care units. The overall risk of bias was high. The pooled sensitivity of the P/F ratio in all included studies for a P/F ratio of 100 was 43.6% (95% CI, 36.9-50.5%) and the specificity was 71.1% (95% CI, 66.7-75.1%) and those for a P/F ratio of 200 were 83.2% (95% CI, 78.2-87.2%) and 26.2% (95% CI, 21.2-31.9%). ConclusionThe P/F ratio had high sensitivity and moderate specificity at a P/F ratio of 200 and 100 respectively., which supports the use of the P/F ratio for screening ARDS patients who are at risk of deterioration.Trial registration: The study was registered in UMIN with registration number 000041058.
Multispectral Active Infrared Thermo-Reflectometry for Absolute Temperature Measurement by Contactless IR Thermography
Authors: Lafargue-Tallet Thomas , VAUCELLE Romain , CALIOT Cyril , AOUALI Abderezak , ABISSET-CHAVANNE Emmanuelle , SOMMIER Alain , PEIFFER Raymond , PRADERE Christophe 
Published: November 2021

Abstract
Knowledge of material emissivity maps and their true temperatures is of great interest for contactless process monitoring and control with infrared cameras when strong heat transfer and temperature change are involved.In this work, we describe the development of a contactless infrared and multispectral imaging technique based on the pyro-reflectometry approach and a specular model of the material reflection.This approach enables in situ and real-time identification of emissivity fields and autocalibration of the radiative intensity leaving the sample by using a black body equivalent ratio.This is done to obtain the absolute temperature field of any specular material using the infrared wavelength.The proposed method is evaluated at room temperature with several heterogeneous samples covering a large range of emissivity values. From these emissivity fields, raw and heterogeneous measured radiative fluxes are transformed into complete absolute temperature fields.
Depression among an Immigrant Black Population of African Origin resident in Canada
Authors: Aluh Deborah , Aigbogun Osaro , Anyachebelu Obinna 
Published: November 2021

Abstract
Background

Lately, there has been a surge of black African-born immigrants to Canada. It is critical to evaluate the extent to which depression has affected this vulnerable and understudied population.
Methods

Participants completed the Patient Health Questionnaire (PHQ-9) depression scale. Descriptive and multivariate logistic regression analyses were carried out using IBM SPPS.
Results

About half (51.7%, n = 91) of the participants met the criteria for depression. Female participants had a significantly higher PHQ-9 score (10.49±4.226) compared to males (8.96± 4.119). Unmarried participants had 27.979 times the odds of being depressed compared to those who were married. Those who had stayed in Canada for more than 10 years had 62.5 times higher odds of being depressed compared to those who had stayed for less than one year.
Conclusions

More than half of the participants exhibited significant depressive symptoms, suggesting an important mental health concern and the need for intervention.
Extracorporeal Perfusion for Free Fillet Lower Leg Flap After External Hemipelvectomy for Recurrent Osteosarcoma: A New Surgical Technique
Authors: Andreani Lorenzo , Mani Olimpia , Ipponi Edoardo , Cosseddu Fabio , Cigna Emanuele , Capanna Rodolfo 
Published: November 2021

Abstract
BackgroundExternal hemipelvectomy often results in exposure of noble structures such as pelvic organs, joint surfaces, iliac neurovascular bundle and bone. Consequently, reconstructive surgery is always challenging. The free fillet lower leg flap is an optimal solution since it allows the transfer of a large amount of tissue and the it determines the absence of donor site morbidities. The prolonged ischemic time represents the weak point of this flap.Methods We presented the case of a 38-year-old man with recurrent radio-induced osteosarcoma arisen on pelvic cavity and involving common iliac vessels, bladder, left ureter and left kidney. We decided to use extracorporeal circulation (ECMO) in order to reduce ischemic time of the flap, since iliac vessels were necessary ligated in the middle of oncologic resection. We perfused the popliteal-based filleted lower leg musculocutaneous free flap with homologue blood and saline solution, while the oncologic dissection was completed.ResultsThe free fillet flap remained vital in the immediate postoperative period and in the following months. The patient did not come back to a life-threatening condition at 1y follow-up. ConclusionWe believed that ECMO expand the reconstructive indications in those cases in which a complex and long-lasting oncologic resection would make impossible a free flap due to prolonged ischemic time.
PRPF6 Induces the Alternative Splicing of lncRNA SNHG16 To Promote Progression and Paclitaxel Resistance of Ovarian Cancer via Regulating GATA3 Transcription
Authors: Wang Han , Zhou Yingying , Zhang Siyang , Qi Ya , Wang Min 
Published: November 2021

Abstract
Background

Small nucleolar RNA host gene 16 (SNHG16) and pre-mRNA processing factor 6(PRPF6) play vital roles in regulatory mechanisms of multiple cancers, but the mechanisms in ovarian cancer (OC) remains poorly understood.
Methods

The expression of SNHG16 transcripts-SNHG16-L/S in OC tissues were analyzed by real-time PCR (RT-PCR). The expression of PRPF6 in OC tissues were detected by Immunohistochemistry (IHC). Tumorigenesis, epithelial-to-mesenchymal transition (EMT) and PTX-resistance were detected by western blot, transwell, CCK-8 assays, colony formation assays and flow cytometry analyses. Molecular interactions were examined by dual-luciferase reporter gene assay, RNA immunoprecipitation (RIP) and chromatin immunoprecipitation (ChIP).
Results

The results indicated the expression of SNHG16-L/S was opposite in chemo-resistance and chemo-sensitivity tissues of OC. And SNHG16-L/S had different effects on the progression and PTX-resistance of OC cells. SNHG16-L inhibited GATA binding protein 3 (GATA3) transcription through CCAAT/enhancer-binding protein b (CEBPB) to further promote tumorigenesis, EMT and PTX-resistance of OC. Moreover, PRPF6 was upregulated in chemo-resistance tissues of OC. PRPF6 promoted tumorigenesis and PTX-resistance in vitro and in vivo. Mechanistically, PRPF6 induced the alternative splicing of SNHG16 to downregulate SNHG16-L, which further mediated progression and PTX-resistance through upregulating GATA3 in OC.
Conclusions

Totally, the results demonstrated that PRPF6 promoted progression and PTX-resistance in OC through SNHG16-L/CEBPB/GATA3 axis. Thus, PRPF6 may become a valuable target for OC therapy.
The structure-activity relationship between precursor fine structure and cathodes performance in Ni-rich layered oxide
Authors: Qiu Lang , Zhang Mengke , Hu Kanghui , Song Yang , Wu Zhenguo , Yue Luchao , Xiang Wei , Ming Yong , Wang Gong-Ke , Liu Yu-Xia , Sun Yan , Zhang Jun , Zhang Bin , Guo Xiaodong 
Published: November 2021

Abstract
Cathode’s primary particle structure plays a key role in the performance of lithium ion batteries, which can be controlled by the precursor synthesis. Regretfully, the relevance between primary particle structure and cathode performance is not explicitly elucidated, that is, what is the discrepancy of cathode’s primary particle size on the structural degradation? In order to elaborate the structure-activity relationship between them, we have systematically investigated the regulation of primary particle size through an in-depth analysis of the precursor growth mechanism, ammonia-stirring coupling and hydrodynamics optimization. Structural and electrochemical characterizations of LiNi0.92Co0.04Mn0.04O2 with different primary sizes (336, 447, 565 and 675 nm) and a rounded analysis of structural degradation after cycling provide insight into the correlation between precursor fine structure and cathode performance, i.e. larger cathode’s primary particle size can effectively inhibit CEI film formation, structure decay, the intragranular/intergranular cracks formation owing to the alleviation of localized stress.
Utility of Three-Protein Panels in the Separation of Aggressive Prostate Cancer from Non-Aggressive Tumors
Authors: Li Qing Kay , Lih Tung-shing Mamie , Wang Yuefan , Hu Yingwei , Hoti Naseruddin , Chan Daniel , Zhang Hui 
Published: November 2021

Abstract
Background

Prostate cancer (PCa) is a heterogeneous group of tumors, including non-aggressive (NAG) and aggressive (AG) subtypes, with variable clinical outcomes. We assessed the diagnostic utility of selected protein markers to identify AG tumors.
Methods

The TMA was constructed, including NAG and AG. 12 protein markers were evaluated using the TMA by IHC stains. The makers were also evaluated for their potential utility as single or panels for distinguishing AG from NAG tumors.
Results

The higher expressions of four protein markers, including prostate specific membrane antigen (PSMA), phospho-EGFR, androgen receptor (AR), and P16, were identified in AG tumors of Gleason score 4 and 5. In contrast, Galectin-3, DPP4 and MAN1B1 revealed stronger staining patterns in NAG tumors. Sensitivity and specificity of individual marker varied widely. In tow-marker panels, especially in the panel of DPP4 and PSMA, the specificity was 38.46% at 95% sensitivity. To further improve the detection ability, we combined DPP4 and PSMA with either Galectin-3 or phospho-EGFR into three-marker panels. The specificity achieved >46% at 95% sensitivity and AUC was >0.85.
Conclusions

Our panels can be used to improve the separation of AG from NAG tumor and to add in the optimization of the treatment strategy for patients.
Web System for Spatial Processing and Analysis: An Application in a Neonatal Screening Program in Southern Brazil
Authors: Oliveira Augusto , Kopacek Cristiane , Castro Simone M. , Vilar Guilherme , Filho Moacyr C. 
Published: November 2021

Abstract
Background

Spatial data analysis refers to the process of finding patterns, detecting anomalies, or testing hypotheses and theories by observing phenomena associated with a specific geographic area or location. The literature in the area presents different studies that seek to understand its phenomena through spatial analysis techniques and methods. However, these studies have several problems, such as the frequent use of only one type of analysis, area or punctual. Furthermore, the studies do not formally describe the process of treatment and organization applied to the data to replicate the spatial analyzes in other research areas. Thus, this work proposes a web system for generating, organizing, and processing data compatible with geographic information systems to construct spatial analysis of area and points.
Methods

The proposed method was developed with the JavaScript programming language and structured in four sequential steps: data acquisition, processing and organization, data validation, and spatial analysis. Data from three diseases (cystic fibrosis, congenital adrenal hyperplasia and hemoglobinopathies) from a neonatal screening program in southern Brazil were used to validate the proposed method and construct the spatial analyses. The choropleth mapping and kernel density estimation methods were used to build the analyses.
Results

The results obtained made it possible to georeference the data, validate it to its area of study, associate it with its micro and mesoregions, and cross it with public databases. In addition, the results enabled the construction of scientific maps of area and points to visualize the primary evidence from the spatial distribution of disease cases.
Conclusions

The developed method showed high replication potential for other study contexts. Also, it proved to be relevant in the context of spatial analysis, enabling speed in processing, data organization and, consequently, in the construction of significant results that can be used in public policies that directly impact people's quality of life and health challenges.
Satisfaction of the Clinical Teachers on Standardized Residency Training Program (SRTP) in China
Authors: Chen Boyan , Jin Xiaoyuan , Zhou Jie , Chen Ying , Wang Hongmei 
Published: November 2021

Abstract
Background: Standardized Residency Training Program (SRTP) is a significant initiative to deepen health systems and medical education in developed countries like China. Despite prompting the SRTP nationwide and implementing it with various improvements, Chinese continuous medical education is still in its infancy. Compared with the residents, little is known about clinical teachers under SRTP in China; but clinical teachers effectively determine the training quality as a critical disseminator of knowledge, skills, and values in medical practice. Thus, the study aims to analyze critical factors affecting their cognitive job satisfaction and provide continuous improvements for SRTP.Methods: From December 1, 2018, to May 31, 2019, we conducted a self-design questionnaire from 13 SRTPs (including both training bases and professional bases) in Shaoxing city to evaluate participants' satisfaction. Altogether, 574 clinical teachers responded to the survey with generally high overall satisfaction. We adopted Chi-square Test and Fisher's Exact Test to evaluate the single impact factors affecting the satisfaction of clinical teachers. The multiple factors analysis applied the Logistic Regression model.Results: The male clinical teachers had significant differences in satisfaction of teaching content (OR: 0.675, [95%CI: 0.477~0.953]), conflicts between study and work (OR: 0.542, [95%CI: 0.371~0.791]), the attention of leaders (OR: 0.403, [95%CI: 0.252~0.645]) and subsidies of teachers (OR: 0.527, [95%CI: 0.347~0.805]). Compared with internal medicine, clinical teachers from Surgery (OR: 2.396, [95%CI: 1.365-4.206]) and other departments (OR: 2.409, [95%CI: 1.406-4.129]) were more satisfied when considered residents have high motivation on training. Besides, compared with attending physician, deputy chief physician (OR: 0.493, [95%CI: 0.310- 0.783]) and chief physician (OR: 0.683, [95%CI: 0.471-0.991]) more disagreed that residents' wage is good enough.Conclusion: Clinical teachers widely recognize the SRTP. However, teachers' satisfaction varied due to different genders, working clinical departments, and professional titles. The study also discussed possible reasons and strategy implications behind these findings, which combined unique Chinese society characteristics. Further, we believe the analysis and interpretations remind us of the applications of Western medical education methods, and theories also should consider the unique socio-cultural challenges.