bluuebunny/crossref_metadata_2025_split huggingface dataset:
On the origin and diversification of the stygobiotic freshwater snail genus Hauffenia (Caenogastropoda: Hydrobiidae) with special focus on the northern species and the description of two new species
Authors: Haase Martin , Grego Jozef , Erőss Zoltán Péter , Farkas Roland , Fehér Zoltán 
Published: October 2021

During systematic surveys of groundwater snails in Slovakia, Hungary and Bosnia and Hercegovina two new species of the genus Hauffenia, H. lozekiana sp. nov. from a single locality in Slovakia, and H. steffeki sp. nov. with a small range in Bosnia and Hercegovina were discovered and are here described based on shell morphology, anatomy and DNA sequence data (COI, 16S rRNA, ITS2). The discovery of H. steffeki sp. nov. extends the range of the genus considerably towards the south. Hauffenia lozekiana sp. nov. appears to be a relict surviving within the range of the today widely distributed H. kissdalmae. Based on a time tree, we developed a scenario for the origin and diversification of the genus. The ancestor probably evolved in the Miocene on the Balkans and with the gradual desiccation of the Paratethys and its remnant water bodies diversified towards the north. Karstic and in particular alluvial connectivities together with changing courses of paleo-rivers probably played an important role for dispersal. Ecological observations suggest that the phreatic rhizosphere, the delicate net of tree rootlets and their exudates, are important for the existence of these groundwater snails.
Drug susceptibility and molecular epidemiology of Escherichia coli in bloodstream infections in Shanxi, China
Authors: Zhang Yanjun , Wang Hairu , Li Yanfang , Hou Yabin , Hao Chonghua 
Published: October 2021

Objectives
We carried out a retrospective study to investigate the drug susceptibility and genetic relationship of clinical Escherichia coli isolates from patients with BSIs in Shanxi, China.

Methods
E. coli isolates causing BSIs were consecutively collected from June 2019 to March 2020. Antimicrobial susceptibility testing was performed by broth microdilution method. PCR was used to detect antimicrobial resistance genes coding for extended-spectrum β-lactamases (ESBLs), phylogenetic groups and seven housekeeping genes of E. coli.

Results
A total of 76 E. coli were collected. Antimicrobial susceptibility testing revealed that the top six E. coli resistant antibiotics were ampicillin (90.7%), ciprofloxacin (69.7%), cefazolin (65.7%), levofloxacin (63.1%), ceftriaxone and cefotaxime (56.5%). Among the 76 isolates, 43 produced ESBLs. Molecular analysis showed that CTX-M-14 was the most common ESBLs, followed by CTX-M-15 and CTX-M-55. Phylogenetic group D (42.2%) predominated, followed by group B2 (34.2%), group A (18.4%) and group B1 (5.2%). The most prevalent sequence types (STs) were ST131 (15/76), ST69 (12/76) and ST38 (6/76).

Conclusions
This study is the first to report the phenotypic and molecular characteristics of E. coli isolated from BSIs in Shanxi, China. Our results indicated a high prevalence of MDR in E. coli strains isolated from BSIs and a serious spread of ESBL genes in Shanxi, especially the epidemiological blaCTX-M. Phylogenetic analysis indicated genetic diversity among E. coli BSIs isolates.
Development and Validation of HPTLC-Densitometric Method Compared to Titrimetric Method for Determination of L-ascorbic Acid in Citrus Fruits
Authors: Dominique Chodaton Zinsou Marthe , Alban Houngbeme Gouton , Habib Ganfon , Mathias Ableto , Djimon Gbenou Joachim , Fernand Gbaguidi 
Published: October 2021

Aims: The aim of this study is to develop and validate an efficient method for the specific determination of L-ascorbic acid in citrus fruits and to compare it with a usual method, method using 2,6-dichlorophenolindophenol
Methodology: The research for a specific method for determination of L-ascorbic acid has led to development and validation of High Performance Thin Layer Chromatography – Densitometric (HPTLC-D) method. The validation criteria evaluated are response function, determination reading wavelength, limit of detection, precision of the technique, limit of quantification and recovery rate. The validated method was applied to citrus juice samples for quantification of L-ascorbic acid. Then a 2,6-dichlorophenolindophenol titrimetric assay of L-ascorbic acid in the same sample was performed for comparison of method results.
Results: HPTLC-D method showed improved sensitivity on 360 ​​nm scanner and a good linearity relationship between standard concentrations and absorbance responses. The regression coefficient obtained is R2 = 0.99. The limit of detection (LOD) and the limit of quantification (LOQ) are respectively 3 ng and 9.5 ng per deposit. The method also exhibited good repeatability and precision, with intra-day (n = 3) and inter-day (n = 4) coefficients of variation (CV) of less than 6%.
Application of the HPTLC-Dmethod to citrus juices yielded a recovery rate ranging from 97% to 99%.
Conclusion: Comparison of results of the two methods shows that the contents obtained by titrimetry are greater than those obtained by HPTLC-D by 42.25%. This is explained by the presence of other redox compounds which are dosed at the same time as L-ascorbic acid. HPTLC-D makes it possible to specifically dose L-ascorbic acid.
Functional Abdominal Pain of Children: Therapy with Bacillus Coagulans GBI-30, 6086 and Insight into Its Probiotic Properties
Authors: Kumar Tandon Vishnu , Dutta Trayambak , Arasan R. Ezhil 
Published: October 2021

Background: Probiotics are effective in the treatment of functional gastrointestinal disorders in adults but there is lack of enough clinical evidence in children.
Aim: To evaluate effectiveness of Bacillus Coagulans GBI-30, 6086 along with digestive enzymes in the treatment of childhood functional abdominal pain (FAP).
Methods: Children with FAP, based on the Rome IV criteria (n = 95, aged 5-16 years), received Bacillus Coagulans GBI-30, 6086 along with digestive enzymes from a commercially available preparation - Tummysoft® for three weeks. Treatment response was assessed by improvement in the Quality of Life in Reflux and Dyspepsia Questionnaire (QOLRAD) score and Global Overall Symptom (GOS) scale.
Results: Patients diagnosed with FAP upon receiving a 3-week treatment with Bacillus Coagulans GBI-30, 6086 along with digestive enzymes, registered statistically significant improvement in both QOLRAD (Baseline, 30.27 ± 5.95; 10th Day: 108.39 ± 7.06; 21st day: 173.71 ± 6.71, P=0.00) and GOS scale (Baseline, 3.10 ± 0.37; 10th Day: 2.15 ± 0.73; 21st day: 1.00 ± 0.00, P = 0) signifying the efficacy of the probiotic in FAP.
Conclusion: Bacillus Coagulans GBI-30, 6086 along with digestive enzymes from a commercially available preparation - Tummysoft® was found to be effective in the treatment of childhood functional abdominal pain (FAP).
3,4,5‐Trimethoxy Substitution on an N‐DMBI Dopant with New N‐Type Polymers: Polymer‐Dopant Matching for Improved Conductivity‐Seebeck Coefficient Relationship
Authors: Han Jinfeng , Chiu Arlene , Ganley Connor , McGuiggan Patty , Thon Susanna M. , Clancy Paulette , Katz Howard E. 
Published: October 2021

AbstractAchieving high electrical conductivity and thermoelectric power factor simultaneously for n‐type organic thermoelectrics is still challenging. By constructing two new acceptor‐acceptor n‐type conjugated polymers with different backbones and introducing the 3,4,5‐trimethoxyphenyl group to form the new n‐type dopant 1,3‐dimethyl‐2‐(3,4,5‐trimethoxyphenyl)‐2,3‐dihydro‐1H‐benzo[d]imidazole (TP‐DMBI), high electrical conductivity of 11 S cm−1and power factor of 32 μW m−1 K−2are achieved. Calculations using Density Functional Theory show that TP‐DMBI presents a higher singly occupied molecular orbital (SOMO) energy level of −1.94 eV than that of the common dopant 4‐(1, 3‐dimethyl‐2, 3‐dihydro‐1H‐benzoimidazol‐2‐yl) phenyl) dimethylamine (N‐DMBI) (−2.36 eV), which can result in a larger offset between the SOMO of dopant and lowest unoccupied molecular orbital (LUMO) of n‐type polymers, though that effect may not be dominant in the present work. The doped polymer films exhibit higher Seebeck coefficient and power factor than films using N‐DMBI at the same doping levels or similar electrical conductivity levels. Moreover, TP‐DMBI doped polymer films offer much higher electron mobility of up to 0.53 cm2 V−1 s−1than films with N‐DMBI doping, demonstrating the potential of TP‐DMBI, and 3,4,5‐trialkoxy DMBIs more broadly, for high performance n‐type organic thermoelectrics.
Mapping the Regioisomeric Space and Visible Color Range of Purely Organic Dual Emitters with Ultralong Phosphorescence Components: From Violet to Red Towards Pure White Light
Authors: Roy Bibhisan , Maisuls Iván , Zhang Jianyu , Niemeyer Felix C. , Rizzo Fabio , Wölper Christoph , Daniliuc Constantin G. , Tang Ben Zhong , Strassert Cristian A. , Voskuhl Jens 
Published: October 2021

AbstractWe mapped the entire visible range of the electromagnetic spectrum and achieved white light emission (CIE: 0.31, 0.34) by combining the intrinsic ns‐fluorescence with ultralong ms‐phosphorescence from purely organic dual emitters. We realized small molecular materials showing high photoluminescence quantum yields (ΦL) in the solid state at room temperature, achieved by active exploration of the regioisomeric substitution space. Chromophore stacking‐supported stabilization of triplet excitons with assistance from enhanced intersystem crossing channels in the crystalline state played the primary role for the ultra‐long phosphorescence. This strategy covers the entire visible spectrum, based on organic phosphorescent emitters with versatile regioisomeric substitution patterns, and provides a single molecular source of white light with long lifetime (up to 163.5 ms) for the phosphorescent component, and high overall photoluminescence quantum yields (up toΦL=20 %).
Mechanochemically Synthesised Flexible Electrodes Based on Bimetallic Metal–Organic Framework Glasses for the Oxygen Evolution Reaction
Authors: Lin Rijia , Li Xuemei , Krajnc Andraž , Li Zhiheng , Li Mengran , Wang Wupeng , Zhuang Linzhou , Smart Simon , Zhu Zhonghua , Appadoo Dominique , Harmer Jeffrey R. , Wang Zhiliang , Buzanich Ana Guilherme , Beyer Sebastian , Wang Lianzhou , Mali Gregor , Bennett Thomas D. , Chen Vicki , Hou Jingwei 
Published: October 2021

AbstractThe melting behaviour of metal–organic frameworks (MOFs) has aroused significant research interest in the areas of materials science, condensed matter physics and chemical engineering. This work first introduces a novel method to fabricate a bimetallic MOF glass, through melt‐quenching of the cobalt‐based zeolitic imidazolate framework (ZIF) [ZIF‐62(Co)] with an adsorbed ferric coordination complex. The high‐temperature chemically reactive ZIF‐62(Co) liquid facilitates the formation of coordinative bonds between Fe and imidazolate ligands, incorporating Fe nodes into the framework after quenching. The resultant Co–Fe bimetallic MOF glass therefore shows a significantly enhanced oxygen evolution reaction performance. The novel bimetallic MOF glass, when combined with the facile and scalable mechanochemical synthesis technique for both discrete powders and surface coatings on flexible substrates, enables significant opportunities for catalytic device assembly.
Design and development of novel series of indole‐3‐sulfonamide ureido derivatives as selective carbonic anhydrase II inhibitors
Authors: Singh Priti , Choli Abhishek , Swain Baijayantimala , Angeli Andrea , Sahoo Santosh K. , Yaddanapudi Venkata M. , Supuran Claudiu T. , Arifuddin Mohammed 
Published: October 2021

AbstractIndole is a privileged moiety with a wide range of bioactivities, making it a popular scaffold in drug design and development studies as well as in synthetic chemistry. Here, novel urea derivatives of indole, containing sulfonamide at position‐3 of indole, were synthesized using a well‐known tail approach, as carbonic anhydrases (CAs; EC 4.2.1.1) inhibitors. All the newly synthesized molecules were screened for their CA‐inhibitory activity against four clinically relevant isoforms of human‐origin carbonic anhydrase (hCA), that is, hCA I, hCA II, hCA IX, and hCA XII. These compounds were specifically active against hCA II, more than against hCA I, hCA IX, and hCA XII. Derivative 6l was found to be most active, with a Ki value of 7.7 µM against hCA II.
Osteogenically differentiated mesenchymal stem cells promote the apoptosis of human umbilical vein endothelial cells in vitro
Authors: Chai Miaomiao , Jiang Mingli , Gu Ce , Lu Qiaohui , Zhou Yi , Jin Ziyang , Zhou Yan , Tan Wensong 
Published: October 2021

AbstractThe absence of blood vessels in tissue engineered bone often leads to necrosis of internal cells after implantation, ultimately affecting the process of bone repair. Herein, mesenchymal stem cells (MSCs) and human umbilical vein endothelial cells (HUVECs) were cocultured to induce osteogenesis and angiogenesis. Based on the findings, the number of HUVECs in the coculture system increased in the growth medium group, but decreased in the osteogenic induction medium (OIM) group. Considering that the paracrine effects of MSCs had changed, we tested the genes expression of osteogenically differentiated MSCs. The expression of osteogenic genes in MSCs increased during osteogenesis. Further, the expression levels of pigment epithelial‐derived factor (PEDF) gene and protein, an antivascular factor, were also increased. To verify whether MSCs promote HUVECs apoptosis via PEDF, PEDF was silenced via siRNA. The conditioned medium of differentiated MSCs with PEDF silencing significantly improved the proliferation and apoptosis of HUVECs. Based on further experiments, PEDF mediated the apoptosis and proliferation of HUVECs through p53, BAX/BCL‐2, FAS, and c‐Caspase‐3. However, when PEDF was silenced with siRNA, the osteogenic potential of MSCs was affected. The results of this study provide a theoretical basis for the construction of prevascularized bone tissues in vitro.
Ein Tragwerk aus Holz für die neue Mensa des Berufsschulzentrums Nord in Darmstadt
Authors: Stumpf Martin , Daub Max 
Published: October 2021

AbstractLebenslanges Lernen in einer zukunftsweisenden Umgebung: Bildung und nachhaltiges Bauen mit Holz auf Basis ausgeklügelter Verfahren sind im Neubau der Mensa vereint. Damit sich Studierende ebenso wie Schüler:innen und die Öffentlichkeit wohl fühlen, wurde interdisziplinär eng zusammengearbeitet zwischen Architektur, Haustechnik und Tragwerksplanung, um das Beste aus allen Bereichen herauszuholen. Der Bericht beschreibt die interdisziplinäre Entwicklung des Holztragwerks sowie die Detaillierung und Ausführung. Ein ähnliches Tragwerk haben die Autoren bereits bei der Festhalle in Neckartailfingen als Dachtragwerk mit 15 m Spannweite und 6 m Auskragung umgesetzt. Die Weiterentwicklung der gitterförmigen Brettschichtholzträgerstruktur für die Mensa in Darmstadt erlaubt den Einsatz der Tragstruktur als Geschossdecke mit einer hohen Nutzlast und sie erfüllt die hohen Anforderungen der Feuerwiderstandsklasse R90. Regelmäßige Trägerroststrukturen haben eine hohe architektonische Qualität. Es entstehen ansprechende Räume, die durch die Tragstruktur geprägt werden. Im Holzbau sind biegesteife Knoten von Balken bzw. Stäben eine besondere Herausforderung und bedürfen einer sorgfältigen Detaillierung dieser Knoten. Der Bericht beleuchtet die Detailentwicklung und beschreibt, wie das gewählte Tragkonzept ohne biegesteife Knoten auskommt.
Prognostic significance of T‐cell–inflamed gene expression profile and PD‐L1 expression in patients with esophageal cancer
Authors: Steiniche Torben , Rha Sun Young , Chung Hyun Cheol , Georgsen Jeanette Baehr , Ladekarl Morten , Nordsmark Marianne , Jespersen Marie Louise , Kim Hyo Song , Kim Hyunki , Fein Carly , Tang Laura H. , Wu Ting , Marton Matthew J. , Peter Senaka , Kelsen David P. , Ku Geoffrey 
Published: October 2021

AbstractPurposeThe ability of the T‐cell–inflamed gene expression profile (GEP) to predict clinical outcome in esophageal cancer (EC) is unknown. This retrospective observational study assessed the prognostic value of GEP and programmed death ligand 1 (PD‐L1) expression in patients with EC treated in routine clinical practice.MethodsTumor samples of 294 patients from three centers in Denmark, South Korea, and the United States, collected between 2005 and 2017, were included. T‐cell–inflamed GEP score was defined as non‐low or low using a cutoff of −1.54. A combined positive score (CPS) ≥10 was defined as PD‐L1 expression positivity. Associations between overall survival (OS) and GEP status and PD‐L1 expression were explored by Cox proportional hazards models adjusting for age, sex, histology, stage, and performance status.ResultsMedian age was 65 years; 63% of patients had adenocarcinoma (AC) and 37% had squamous cell carcinoma (SCC). Thirty‐six percent of tumors were GEP non‐low, with higher prevalence in AC (46%) than SCC (18%). Twenty‐one percent were PD‐L1–positive: 32% in South Korean samples versus 16% in non‐Asian samples and 26% in SCC versus 18% in AC. GEP scores and PD‐L1 CPS were weakly correlated (Spearman’s R = 0.363). OS was not significantly associated with GEP status (non‐low vs low; adjusted hazard ratio, 0.91 [95% CI, 0.69–1.19]) or PD‐L1 expression status.ConclusionNeither GEP nor PD‐L1 expression was a prognostic marker in Asian and non‐Asian patients with EC.
Linear Selective Hydroformylation of 2‐Arylpropenes Using Water‐Soluble Rh‐PNP Complex: Straightforward Access to 3‐Aryl‐Butyraldehydes
Authors: Ru Tong , Liang Guanfeng , Zhang Luyun , Ning Yingtang , Chen Fen‐Er 
Published: October 2021

AbstractStraightforward access to 3‐aryl‐butyraldehydes was developed through the aqueous biphasic Rh‐catalyzed hydroformylation of 2‐arylpropenes using a water‐soluble PNP ligand. This protocol accommodates broad substrate scope with high yields (up to 95 %) and excellent linear selectivity (>99 : 1 b/l ratio). The synthesis of rac‐ar‐turmerone and the gram‐scale hydroformylation further demonstrated the practical nature of this method.
Screening of Minimalist Noncanonical Sites in Duplex DNA and RNA Reveals Context and Motif‐Selective Binding by Fluorogenic Base Probes
Authors: Liang Yufeng , Miao Shiqin , Mao Jie , Devari Shekaraiah , Gonzalez Maricarmen , Bong Dennis 
Published: October 2021

AbstractWe hypothesize that programmable hybridization to noncanonical nucleic acid motifs may be achieved by macromolecular display of binders to individual noncanonical pairs (NCPs). As each recognition element may individually have weak binding to an NCP, we developed a semi‐rational approach to detect low affinity interactions between selected nitrogenous bases and noncanonical sites in duplex DNA and RNA. A set of fluorogenic probes was synthesized by coupling abiotic (triazines, pyrimidines) and native RNA bases to thiazole orange (TO) dye. This probe library was screened against duplex nucleic acid substrates bearing single abasic, single NCP, and tandem NCP sites. Probe engagement with NCP sites was reported by 100–1000× fluorescence enhancement over background. Binding is strongly context‐dependent, reflective of both molecular recognition and stability: less stable motifs are more likely to bind a synthetic probe. Further, DNA and RNA substrates exhibit entirely different abasic and single NCP binding profiles. While probe binding in the abasic and single NCP screens was monotonous, much richer binding profiles were observed with the screen of tandem NCP sites in RNA, in part due to increased steric accessibility. In addition to known binding interactions between the triazine melamine (M) and T/U sites, the NCP screens identified new targeting elements for pyrimidine‐rich motifs in single NCPs and 2×2 internal bulges. We anticipate that semi‐rational approaches of this type will lead to programmable noncanonical hybridization strategies at the macromolecular level.
Risk of suicide among individuals with a history of childhood cancer
Authors: Barnes Justin M. , Johnson Kimberly J. , Grove Jeremy L. , Srivastava Amar J. , Osazuwa‐Peters Nosayaba , Perkins Stephanie M. 
Published: October 2021

BackgroundPrevious studies have described suicidal ideation among survivors of childhood cancer, but small numbers of events limit the understanding of suicide risk. The objectives of this study were to assess whether childhood cancer survivors are at increased risk of suicide in comparison with the general population and to determine risk factors associated with risk in a population‐based cohort.MethodsFirst primary malignancies among individuals aged 0 to 19 years from 1975 to 2016 were identified from Surveillance, Epidemiology, and End Results (SEER) databases. Standardized mortality ratios (SMRs) of suicide were obtained via SEER*Stat software from SEER 9. Fine and Gray proportional hazards models were used to identify suicide‐associated factors among childhood cancer patients included in SEER 18.ResultsIn all, 96,948 childhood cancer cases and 89 suicides were identified. Across all attained ages, the suicide risk for individuals with a childhood cancer history (11.64 per 100,000 person‐years) was similar to the risk for those without a cancer history (SMR, 1.14; 95% confidence interval [CI], 0.91‐1.43). However, for survivors alive beyond the age of 28 years (the median age of death by suicide), the suicide risk was significantly elevated (suicides per 100,000 person‐years, 22.43; SMR, 1.40; 95% CI, 1.02‐1.87). Females (hazard ratio, 0.29; 95% CI, 0.18‐0.59; P < .01) had lower risks than males.ConclusionsThese results suggest that long‐term childhood cancer survivors may be at increased suicide risk. Male sex is an independent risk factor for suicide. However, the absolute risk of suicide in older survivors is still low at ~1 per 5000 person‐years. Future efforts should identify survivorship strategies to mitigate suicide risk.
No self‐serving bias in therapists' evaluations of clients' premature treatment termination: An approximate replication of Murdock et al. (2010)
Authors: Dandachi‐FitzGerald Brechje , Meijs Laura , Moonen Isabelle M. A. J. , Merckelbach Harald 
Published: October 2021

AbstractIn an often‐cited study, Murdock et al. (2010) found that therapists are more likely to attribute premature treatment termination to client characteristics than to themselves, a finding that the authors interpreted in terms of a self‐serving bias (SSB). We replicated and extended the study of Murdock et al. (2010, study 2). Psychologists and psychotherapists (N = 91) read two case vignettes about premature treatment terminations of clients that, in a between‐subjects set‐up, were either described as own clients or other therapists' clients. Next, participants used three attribution subscales (blaming therapist, client and situation) to evaluate potential causes for the premature terminations. This way, we tested whether participants would manifest SSB. We also investigated whether therapists' scores on self‐confidence and need for closure were linked to SSB tendencies. Unlike Murdock et al. (2010), we found no overall SSB. However, a stronger need for closure was related to more SSB tendencies (i.e., less endorsement of ‘blame therapist’ attributions) in the own‐client condition (r = −.35, p < .05, r2 = .12), but not in the other‐therapist's‐client condition (r = .17, p = .27). Our results suggest that SSB is not a ubiquitous phenomenon when therapists evaluate premature termination problems and that their willingness to attend to their own role depends to some extent on their need for closure.