bluuebunny/crossref_metadata_2025_split huggingface dataset:
Plasticity To Environmental Conditions And Substrate Colonization Mediates The Invasion of Dreissenid False Mussels In Brackish Systems
Authors: Rodrigues Antonio J. S. , Miyahira Igor Christo , Rodrigues Nathália , Ribeiro Danielle , Santos Luciano Neves , Neves Raquel A. F. 
Published: August 2021

Abstract
False mussels are recognized as the brackish water equivalent of zebra mussels, although the abiotic and habitat conditions that mediate these invaders’ success are barely known. In this context, we aimed to evaluate the native and non-native geographical distribution of Mytilopsis species worldwide and assess biological traits, environmental condition, and habitat associated with false mussels in native and invaded systems. Our hypothesis is that Mytilopsis invasion is driven by species plasticity to environmental conditions and substrate use in brackish systems, where the colonization of non-native populations is favored by great availability of artificial substrates and tolerance to wide ranges of environmental conditions. Besides, this study provides the occurrence range and distribution patterns of Mytilopsis species within their introduced and native areas and tracks the spread of introduced populations worldwide. Considering the five species evaluated, M. leucophaeata and M. sallei are the most widespread, while M. adamsi, M. trautwineana, and M. africana showed more restricted geographic distribution. In the last decades, M. leucophaeata and M. sallei consolidated and expanded their distributions. Environmental conditions were significantly different between native and non-native areas, where Mytilopsis populations presented significantly higher densities. Non-native populations exhibited remarkable plasticity concerning habitat colonization that was more frequent on artificial substrata. Mytilopsis populations presented significant differences on their biological traits, habitat environmental conditions, and substrate use between native and non-native areas. These species seem to adapt to the conditions of invaded systems, changing their preferences, which reflects plasticity and suggests a potential shift of their realized niches.
MicroRNA-Regulated Ubiquitination and Lipid Metabolism Networks are Associated with Chemotherapy Response in Ovarian Cancer
Authors: Topouza Danai Georgia , Choi Jihoon , Nesdoly Sean , Tarnouskaya Anastasiya , Nicol Christopher J.B. , Duan Qing Ling 
Published: August 2021

Abstract
High-grade serous ovarian cancer (HGSOC) is a highly lethal gynecologic cancer, in part due to resistance to platinum-based chemotherapy reported among 20% of patients. This study aims to characterize the biological mechanisms underlying chemotherapy resistance, which remain poorly understood.Analysis of mRNA/microRNA sequencing data from HGSOC patients of The Cancer Genome Atlas identified 196 differentially expressed mRNAs enriched for adaptive immunity and translation, and 21 differentially expressed microRNAs associated with angiogenesis. Co-expression network analysis identified two mRNA networks associated with chemotherapy response, which were enriched for ubiquitination and lipid metabolism, as well as three associated microRNA networks enriched for lipoprotein transport and oncogenic pathways. These network modules replicated in two independent ovarian cancer cohorts. Moreover, integrative analyses of the mRNA, microRNA and genomics (i.e. single nucleotide polymorphisms) datasets revealed potential regulation of the mRNA networks by the associated microRNAs and SNPs (i.e. expression quantitative trait loci).Thus, we report novel transcriptional networks and biological pathways associated with resistance to platinum-based chemotherapy among HGSOC patients. These results improve our understanding of the effector networks and regulators of chemotherapy response, which will help to elucidate novel therapeutic targets for ovarian cancer.
Obstructive Sleep Apnea and Associated Factors Among Hypertensive Patients Attending a Tertiary Cardiac Center in Tanzania: a Comparative Cross-sectional Study.
Authors: Pallangyo Pedro , Mgopa Lucy R. , Mkojera Zabella , Komba Makrina , Millinga Jalack , Misidai Nsajigwa , Swai Happiness J. , Mayala Henry , Bhalia Smita , Wibonela Salma , Janabi Mohamed 
Published: August 2021

Abstract
Background: There is mounting evidence for a reciprocal yet bidirectional association between sleep-disordered breathing and hypertension. Obstructive sleep apnea (OSA), a common cause of systemic hypertension is an independent risk factor for hypertension-related cardiovascular morbidity and mortality. In this comparative hospital-based cross-sectional study, we sought to explore the burden of obstructive sleep apnea and its associated risk factors among hypertensive patients attending Jakaya Kikwete Cardiac Institute.Methodology: A total of 1974 individuals (i.e. 1289 hypertensive and 685 normotensives) were consecutively enrolled in this study. The Berlin questionnaire and Epworth Sleepiness Scale were utilized in the assessment of OSA and excessive daytime sleepiness (EDS) respectively. Logistic regression analyses were employed in the determination of associated factors for OSA. Results: The mean age was 53.4 years and females constituted the large majority (60.4%) of participants. About three quarters (74.1%) of participants had excess body weight, 11.6% had diabetes, 8.0% had asthma and 18.6% had history of recurrent nasal congestion. Positive family history of snoring was reported by 43.1% of participants and 36.9% had a personal history of snoring. Persons with hypertension displayed a higher frequency (42.1%) of OSA compared to their normotensive counterparts (11.8%), p<0.001. Multivariate logistic regression analyses revealed hypertension (OR 5.1, 95% CI 3.2-8.2, p<0.001), diabetes mellitus (OR 2.2, 95% CI 1.3-3.5, p<0.01), chronic nasal congestion (OR 1.6, 95% CI 1.1-2.5, p = 0.01), obesity (OR 2.4, 95% CI 1.8-3.3, p<0.001), increased neck circumference (OR 2.7, 95% CI 1.2-6.4, p = 0.02), family history of snoring (OR 5.5, 95% CI 4.0-7.5, p<0.001), and working >8hrs/24hr (OR 0.6, 95% CI 0.4-1.0, p = 0.03) to have an independent association for OSA. Furthermore, participants with hypertension displayed superior odds for OSA compared to their normotensive counterparts across all subgroup analyses. Conclusion: OSA is considerably common among hypertensives in a tertiary health care setting in Tanzania. Positive family history of snoring was the strongest associated factor; however, excess body weight proved to be the strongest modifiable risk factor. In view of its pervasiveness, OSA should be an integral part of the medical evaluation in hypertensive individuals.
Torula Yeast in the Diet of Atlantic Salmon Salmo Salar and the Impact on Growth Performance and Gut Microbiome.
Authors: Leeper Alexandra , Ekmay Ricardo , Knobloch Stephen , Skírnisdóttir Sigurlaug , Varunjikar Madhushri , Dubois Marianne , Smárason Birgir Örn , Árnason Jón , Koppe Wolfgang , Benhaïm David 
Published: August 2021

Abstract
Atlantic salmon aquaculture is expanding, and with it, the need to find suitable replacements for conventional protein sources used in formulated feeds. Torula yeast (Cyberlindnera jadinii), has been identified as a promising alternative protein for feeds and can be sustainably cultivated from lignocellulosic biomasses. The present study investigates the impact of torula yeast on the growth performance and the gut microbiome of freshwater Atlantic salmon. Both a marine protein base diet and a mixed marine and plant protein base diet were tested, where conventional proteins were replaced with increasing inclusion levels of torula yeast, (0%, 10%, 20%). This study demonstrated that 20% torula yeast can replace fish meal without alteration to growth performance while leading to potential benefits for the gut microbiome by increasing the presence of bacteria positively associated with the host. However, when torula yeast replaced plant meal in a mixed protein diet, results suggest 10% inclusion of yeast produced the best growth performance results and at the highest inclusion level, potentially negative changes were observed in the gut microbial community, such as a decrease in lactic acid bacteria. This study supports the continued investigation of torula yeast for Atlantic salmon as a partial replacement for conventional proteins.
Cellular Senescence and Senescence-Associated Secretory Phenotype Drive Multipotent Cardiac Stem/Progenitor Cell Dysfunction in Human Diabetic Cardiomyopathy Independently of Age
Authors: Marino Fabiola , Scalise Mariangela , Cappetta Donato , Salerno Luca , Molinaro Claudia , Salerno Nadia , Torella Michele , Greco Marta , Foti Daniela , Sasso Ferdinando C. , Mastroroberto Pasquale , De Angelis Antonella , Ellison-Hughes Georgina M. , Rota Marcello , Urbanek Konrad , Torella Daniele , Cianflone Eleonora 
Published: August 2021

Abstract
Background: Aging and Diabetes Mellitus (DM) independently and additively increase cardiovascular risk and a pathophysiological basis for this epidemiological link is currently the subject of intense investigations. Both aging and DM affect the biology and regenerative potential of tissue specific cardiac adult stem/progenitor cells (CSCs). In aged subjects over half of the CSCs are senescent with a senescence-associated secretory phenotype (SASP) that renders otherwise healthy CSCs to senescence, impairing their proliferative and differentiation potential. Although a link to organismal aging is clear, cells can undergo senescence, regardless of age. Yet, it is unclear whether the SASP is induced by Diabetes per se in CSCs and whether targeting senescent cells within the diabetic CSC compartment rescues their regenerative biology. Methods: In order to investigate the effects of DM on CSC senescence and to try separate those of aging from Diabetes on senescence events, we obtained peri-infarct/border zone biopsies from non-aged patients (50-64 years old) with DM type 2 (T2DM) and non-diabetic (NDM) patients with post-infarct cardiomyopathy undergoing surgical coronary revascularization. Results: Ischemic injury in DM is associated with a higher ROS production, as revealed by the exacerbated expression of 8-OH-deoxyguanosine, nitrotyrosine, and 4-hydroxinonenal targeting both cardiomyocytes as well as CSCs. The latter associated with an increased number of senescent and dysfunctional T2DM-hCSCs (isolated from atrial samples of T2DM and NDM patients with ischemic cardiomyopathy) identified by increased p16INK4a positive cells, reduced telomerase activity and telomere length, reduced proliferation, clonogenesis/spherogenesis and myogenic differentiation when compared to NDM-hCSCs in vitro. Importantly, T2DM-hCSCs show a defined SASP, as demonstrated by the increased secretion of MMP-3, PAI1, IL-6, IL-8, IL-1β and GM-CSF. A combination of two senolytics, Dasatinib and Quercetin, clears senescent T2DM-hCSCs in vitro restoring expansion and myogenic differentiation capacities of the diabetic hCSC pool. Conclusions: DM hampers human CSC biology, inducing a variety of hallmarks of senescence in non-aged subjects that contribute to the deficit of their regenerative potential. Clearance of senescent cells by senolytics abrogates the SASP and restores a fully proliferative- and differentiation- competent hCSC pool in T2DM.
Factors Influencing Food Choices In Different Sports: Importance of Sustainability, Performance And Health: A Cross-Sectional Study
Authors: Nuszbaum Cosima , Wollmar Mari , Sjöberg Agneta 
Published: August 2021

Abstract
Background: This study investigates food preferences among individuals active in different sports. Since food intake affects the carbon footprint, it is of interest to investigate influencing factors of food choices regarding sustainability. Physically active individuals are in need for high intakes of energy and protein in order to maintain their performance. Little is known about what influence food choices in this population. Method: A survey of a sample of 367 individuals was conducted in German and Swedish gyms, climbing halls and CrossFit boxes. Therefore, a questionnaire was developed to capture information about factors influencing food choice and consumed protein sources. Kruskal-Wallis-test tested significant differences between sport groups and physical activity levels.Results: The most frequently consumed animal-based protein were dairy-products, and the most frequently used plant-based source were grains. Taste and health were the main influences on food choices. Significant differences were observed between sport groups and physical activity levels. Sustainability was ranked as moderately important, with the highest importance in people active in the outdoor sports running and climbing. Participants with high physical activity levels and active in CrossFit rated performance more important than the rest of the sample and consumed protein supplements, eggs and poultry more frequently. Conclusion: Differences in food choices between various sports and physical activity levels were identified. Understanding the underlying influences on food choices might help to motivate a shift towards a more sustainable diet in athletes.
Gpr Application for the Characterization of Sinkholes in Teresina, Brazil
Authors: Lago Alexandre Lisboa , Borges Welitom Rodrigues , Barros José Sidney , Amaral Elizângela de Sousa 
Published: August 2021

Abstract
The town of Teresina, in the state of Piauí, Brazil, presents a history of land sinking processes. Two sinking events deserve to be highlighted, the first occurred in 1999 at Simplício Mendes street and the other occurred on July 31, 2008, at Francisco Mendes street. In order to identify possible shallow caves and associated structures, the Geological Survey of Brazil/CPRM developed a Ground Penetrating Radar (GPR) study to verify the occurrence of reflection patterns characteristic of these dissolution structures. In the field, the team conducted GPR sections along streets with a history of collapse. The GPR results obtained with shielded antennas of 200MHz allowed the identification of old areas of collapse of the terrain, to a maximum depth of 3 meters. In addition, the results obtained by this study show the potential of applying the GPR method in the characterization of the subsoil of paved streets, making it possible to identify various layers: asphalt, actual pavement, subgrade pavement, soil, saprolite, and mixed material. The high clay content of the subsoil does not allow the GPR to investigate further depths in the research area. The interpretation of aeromagnetometric data shows that the occurrence of sinkholes is associated with magnetic lineaments mainly in the NW-SE direction and enhances the understanding of the structural framework of the study area.
Karst Geology in Southwest Hubei and Mitigation of Geohazards During Mountain Tunnel Through a Large Karst Cave
Authors: Li Yan-Wei , Lai Hong-Peng , Wu Qiang , Sun Jie 
Published: August 2021

Abstract
The outcrop area of carbonate rocks accounts for 61.6% of the total area in Southwest Hubei. The action of tectonic application, erosion of surface and underground water system, topography, and carbonate geology leads to the formation of large karst caves, and their features are as following: considerably large, long and narrow, winding and in different forms. Limited by route directions, large karst caves would be encountered during tunneling through mountains, therefore causing hazards such as instability of the karst cave, water or stone ingress, damage to the tunnel, and long-term instability. A case study of mountain tunnel through a large karst cave is presented. The large karst cave is formed due to the erosion of limestone by two underground river systems. The length, width and volume of the cavity of the large karst cave are about 139 m, 91 m and 4.3×105 m3. The large karst cave is overall stable without strong external factors. Engineering proposals and treatment of the large karst cave are introduced. The small pipe grouting method is used to treat the foundation. Two kinds of lining structure schemes are adopted, double lining open-cut tunnel structure, and traditional tunnel structure after backfill. The water outside the lining of the open-cut tunnel is introduced into the tunnel ditch and discharged through the tunnel drainage system. Monitoring and measurement during tunneling construction and operation process are introduced.
A Novel Pyroptosis-related Gene Signature for Prognostic Prediction of Head and Neck Squamous Cell Carcinoma
Authors: Qian Xiaoyu , Tang Jian , Chu Yongquan , Chen Ziqiang , Chen Liang , Shen Chao , Li Lin 
Published: August 2021

Abstract
Background: Head and neck squamous cell carcinoma (HNSCC) is an extremely heterogeneous malignant cancer with poor prognosis. Pyroptosis is defined as a novel inflammation-dependent programmed cell death. However,the pyroptosis-associated genes expression in HNSCC and their relationship with prognosis is still indistinct.Material and Methods: We acquired HNSCC patients’ mRNA expression information from TCGA and GEO publicly available. We compared tumor issues and adjacent normal tissues in terms of the gene expression, for identifying differentially expressed genes (DEGs). Based on these genes, we established a risk signature by the LASSO Cox regression in the TCGA cohort and validated in a GEO cohort. We also verified the levels of relevant mRNA expression in the model by RT-qPCR. Eventually, functional enrichment approach was carried out to explore the potential mechanisms.Results: Our team found a total of 18 differentially expressed genes (DEGs) between HNSCC and healthy sample, and 4 DEGs displayed a remarkable association with the overall survival (OS) (P< 0.05). A 4-gene signature was constructed, presenting beneficial forecast power both in TCGA and GEO cohort. Our team categorized cases into a group with high risk and another group with low risk in the light of the average risk value of the 4-gene feature. Cases in the low risk group displayed a notably greater OS compared with the high risk one (P< 0.01). The Cox regression study demonstrated the independent forecast capability of the risk score. The receiver operating characteristic approach facilitated the verification of the forecast function of the gene signature. Posterior to verification, the 4 genes were aberrantly expressed in HNSCC and healthy samples.Functional study displayed that these groups registered diverse immunity conditions. Conclusions: Pyroptosis-associated genes paly an vital role in the prognosis of HNSCC and could be an potential therapeutic target.
Gut microbiota modulates lung fibrosis severity following acute lung injury
Authors: Chioma Ozioma , Hesse Laura , Mallott Elizabeth , Chapman Austin , Amburg Joseph Van , Wu Hongmei , Shah-Gandhi Binal , Piazuelo M. Blanca , Johnson Joyce , Bernard Gordon , Bodduluri Sobha , Davison Steven , Haribabu Bodduluri , Bordenstein Seth , Drake Wonder 
Published: August 2021

Abstract
Independent reports note the significance of gut microbiota on lung disease severity; however, studies using murine models to define the role of the gut microbiome in pulmonary fibrosis progression are missing. We used the bleomycin murine model to quantify lung fibrosis in C57BL/6J mice housed in germ-free, animal biosafety level 1 (ABSL-1), or animal biosafety level 2 (ABSL-2) environments. Mice housed in gnotobiotic facilities are protected from bleomycin-induced pulmonary fibrosis, while ABSL-1 and ABSL-2 mice develop mild fibrosis and severe lung fibrosis, respectively. Metagenomic analysis of the gut microbiota revealed greater microbial diversity in ABSL-1 compared to ABSL-2 mice, with an increased presence of Lactobacilli and Bifidobacterium in ABSL-1 mice. Flow cytometric analysis of single-cell lung suspensions revealed enhanced IL-6/STAT3 /IL-17A signaling in CD4+ T cells of ABSL-2 mice, compared to ABSL-1 or germ-free mice. Fecal microbiota transplantation (FMT) of low microbial diverse stool (ABSL-2) into germ-free mice before bleomycin administration recapitulated the severe fibrosis phenotype, whereas FMT of ABSL-1 stool induced minimal fibrosis. These findings strongly support a causal role of the gut microbiota in augmenting pulmonary fibrosis severity after acute lung injury.
Meditation-Induced Effects on Whole-Brain Structural and Effective Connectivity
Authors: De Filippi Eleonora , Escrichs Anira , Càmara Estela , Garrido César , Sánchez-Fibla Martí , Gilson Matthieu , Deco Gustavo 
Published: August 2021

Abstract
In the past decades, there has been a growing scientific interest in
characterizing neural correlates of meditation
training. Nonetheless, the mechanisms underlying meditation remain elusive.
In the present work, we investigated meditation-related changes in structural and functional connectivities (SC and FC, respectively). For this purpose, we scanned experienced meditators and control (naive) subjects using magnetic resonance imaging (MRI) to acquire structural and functional data during two conditions, resting-state and meditation (focused attention on breathing). In this way, we aimed to characterize and distinguish both short-term and long-term modifications in the brain's structure and function. First, we performed a network-based analysis of anatomical connectivity. Then, to analyze the fMRI data, we calculated whole-brain effective connectivity (EC) estimates, relying on a dynamical network model to replicate BOLD signals' spatio-temporal structure, akin to FC with lagged correlations. We compared the estimated EC, FC, and SC links as features to train classifiers to predict behavioral conditions and group identity. The whole-brain SC analysis revealed strengthened anatomical connectivity across large-scale networks for meditators compared to controls. We found that differences in SC were reflected in the functional domain as well. We demonstrated through a machine-learning approach that EC features were more informative than FC and SC solely. Moreover, we showed that the most informative EC links that discriminated between meditators and controls involved the same large-scale networks previously found to have increased anatomical connectivity.
Overall, the results of our whole-brain model-based approach revealed a mechanism underlying meditation by providing causal relationships at the structure-function level.