bluuebunny/crossref_metadata_2025_split huggingface dataset:
PSIV-B-25 Digestible energy and metabolizable energy of high-fiber ingredients in growing pig diets
Authors: Kim Jongkeon , Jo Yun Yeong , Kim Beob Gyun G 
Published: October 2021

Abstract
The objective of this study was to determine the digestible energy (DE) and metabolizable energy (ME) concentrations in high-fiber ingredients fed to growing pigs. Twelve barrows with an initial body weight of 57.5 kg (SD = 5.7) were individually housed in metabolism crates. A replicated 6 × 3 incomplete Latin square design with 12 animals, 6 experimental diets and 3 periods was employed. A basal diet was composed of 75.0% corn and 22.7% soybean meal (SBM) as the sole energy sources. Four experimental diets were prepared by replacing 40% of corn and SBM with soybean hulls (SH), corn gluten feed (CGF), wheat bran (WB), or rice bran (RB). An additional diet was prepared by replacing 10% of corn and SBM with cashew nut hulls (CNH). Each period consisted of a 4-d adaptation period and a 4-d collection period, and the marker-to-marker procedure was used for total collection of feces and urine. The DE and ME values in RB (3,969 and 3,936 kcal/kg DM) were greater (P &lt; 0.05) than those in CGF (2,654 and 2,520 kcal/kg DM) and SH (2,492 and 2,541 kcal/kg DM) and the energy values in WB (3,162 and 3,118 kcal/kg DM) were not different from those in RB, CGF, or SH. The DE and ME values in CNH (350 and 572 kcal/kg DM) were less (P &lt; 0.05) than those in all other test ingredients. In conclusion, energy concentrations in RB were greatest among the high-fiber test ingredients, whereas CNH had the lowest values.
PSIV-B-22 Comparative effects of nanoparticle-selenium, selenium-enriched yeast and sodium selenite antagonize cadmium-induced cardiotoxicity in chicken (Gallus gallus) via modulating AHR/CAR/PXR/Nrf2 pathways
Authors: Ge Jing , Huang Yan , Li Jinlong 
Published: October 2021

Abstract
Selenium (Se), a nutritionally essential mineral for human and animals, has a significant antagonistic effect on heavy metal cadmium (Cd) biotoxicity. Still, the impact of different Se source on alleviating Cd toxicity has received only limited attention. Therefore, the purpose of the current study was to assess the mitigation level of Cd-induced cardiotoxicity by different sources such as nanoparticles of selenium (Nano-Se), selenium-rich yeast (SY) and sodium selenite (SS). Two hundred of male, 1-day old Hy-Line Variety White chickens were randomly, equally divided into five groups (n = 25). These chicken groups were assigned to (1) basal diet (Con), (2) 140 mg/kg CdCl[2] of basal diet Cd (Cd), (3) 1 mg/kg Nano-Se plus 140 mg/kg CdCl[2] of basal diet (Nano-Se + Cd), (4) 3 mg/kg selenium-enriched yeast plus 140 mg/kg CdCl[2] of basal diet (SY + Cd group), and (5) 3 mg/kg sodium selenite plus 140 mg/kg CdCl[2] of basal diet (SS+ Cd group). The results evidenced that presence of Cd led to a significant increase in biochemical parameters such as lactate dehydrogenase (LDH) and creatine kinase (CK), as well as histopathological lesions in the heart of chickens. Cd exposure also resulted in more extensive effects on phase I metabolism enzymes and transcript CYP isoforms, elevated the levels of MDA and H[2]O[2] and depressed total superoxide dismutase (T-SOD), Cu-Zn SOD, total antioxidant capacity (T-AOC) and catalase (CAT) activities. The expression of nuclear receptors (NRs), AHR, CAR and PXR was declined, down-regulated Nrf2 and its downstream targets in the Cd-treat group. Notably, Se sources application alleviated Cd toxicity by triggering AHR/CAR/PXR/Nrf2 signaling pathway to promote restoring antioxidant defense system and phase I metabolism enzymes system. However, when compared the effectiveness of antagonism, the Nano-Se was superior in relieving Cd-induced cardiotoxicity via AHR/CAR/PXR/Nrf2 pathway activation than other Se- sources.
PSVI-3 Effect of feeding resistant starch on sow and piglet farrowing performance and blood parameters
Authors: Markland Mary L , Lay Donald C , Richert Brian T 
Published: October 2021

Abstract
Hyperprolific sows can have a long duration of farrowing, negatively impacting piglet survival and vitality. This study’s objective was to assess the effect of a diet containing 6.6% resistant starch (RS) on sow and piglet farrowing performance. We hypothesized that a diet containing RS would decrease sow farrowing duration and improve piglet survival. Forty-two sows were balanced for parity and randomly assigned to 1 of 2 treatments: standard lactation diet (n = 21) or diet containing RS (n = 21). Sows were fed their respective diets from approximately 7d prefarrowing throughout lactation. Sow blood was drawn at three time points on d106±1.0 and d113±1.0 of gestation (pre-feeding, 2 and 6 h post-feeding) and during farrowing to measure blood glucose. Piglet umbilical blood (~3/litter; beginning, middle, end of birth order) was collected at birth and analyzed for glucose, lactate, pH, PCO2, PO2, TCO2, HCO3, BE, and sO2. Piglets were visually assessed at birth to score meconium staining. Data collected included farrowing duration, piglet birthing intervals, number of stillborn piglets/litter, sow and piglet weights, piglet mortality, and sow feed and water intake. Average litter sizes post-crossfoster for control and RS were similar, 12.1±0.4 and 11.6±0.4 piglets, respectively. Litter weight gain was similar between control and RS (44.97 vs. 47.54 kg, P = 0.33), respectively. Feeding resistant starch had no effect on number of stillborn piglets or pre-weaning survival (P &gt; 0.75). Farrowing duration (178.9±17.6 min vs 165.2±18.0 min, P = 0.59) and piglet birth intervals (16.57±1.7 min and 14.89±1.8 min, P = 0.51) did not differ between control and RS, respectively. There was difference in sow blood glucose between diets (P = 0.02), day of sample (P &lt; 0.0001), time point (P &lt; 0.0001), and a day by time point interaction (P &lt; 0.0001). Feeding RS to sows prefarrowing can modify sow blood glucose, but did not result in reduced farrowing duration or improved piglet survival.
Long non-coding RNA AFAP1-AS1 promotes thyroid cancer progression by sponging miR-204-3p and upregulating DUSP4
Authors: Ma Hongzhi , Shi Qian , Fang Jugao , Wang Ru , Zhao Jianyu , Lin Sitong , Dong Jiajing , Zhang Yan , Shen Xixi , Chen Jiaming , Zhong Qi 
Published: October 2021

Abstract
Long non-coding RNA actin filament-associated protein 1-antisense RNA 1 (AFAP1-AS1) shows crucial regulatory function in tumor progression. Nonetheless, the biological function and underlying mechanism of AFAP1-AS1 in the progression of thyroid cancer is still unclear. Expressions of AFAP1-AS1, miR-204-3p and DUSP4 were quantified utilizing quantitative real-time polymerase chain reaction and/or western blot. In loss-of-function and gain-of-function assays, cell proliferation, migration and invasion were appraised by CCK-8 assay, wound healing assay, Transwell migration and invasion assays, respectively. Luciferase reporter assay was employed for validating the interaction between miR-204-3p and AFAP1-AS1 or the 3’UTR of dual specificity phosphatase 4 (DUSP4). AFAP1-AS1 was highly expressed in thyroid cancer tissues and cell lines. Highly expressed AFAP1-AS1 was in association with advanced TNM stage and positive lymph node metastasis. Knockdown of AFAP1-AS1 suppressed the proliferation, migration and invasion of thyroid cancer cells, and overexpression of AFAP1-AS1 induced a reversed effect. MiR-204-3p was targetedly repressed by AFAP1-AS1, and miR-204-3p could negatively regulate DUSP4 expression. AFAP1-AS1 augmented the expression of DUSP4 via repressing miR-204-3p, and the effects of AFAP1-AS1 overexpression on thyroid cancer cells were also partly abolished by miR-204-3p restoration. In summary, AFAP1-AS1 facilitates thyroid cancer cell proliferation, migration and invasion by regulating miR-204-3p/DUSP4 axis.
Forty years of the structure of plant-type ferredoxin
Authors: Kurisu Genji , Tsukihara Tomitake 
Published: October 2021

AbstractThe X-ray structure of a [2Fe-2S]-type ferredoxin (Fd) from Spirulina platensis, solved by a collaborative group led by Profs Masao Kakudo, Yukiteru Katsube and Hiroshi Matsubara, was the first high-resolution structure of a plant-type Fd deposited in the Protein Data Bank. The main chain structure, comprising a [2Fe-2S] cluster ligated by four conserved cysteine residues, together with a molecular evolutionary study based on a series of amino acid sequence determinations, was reported in Nature in 1980. The consequent detailed crystallographic analysis, including crystallization, heavy atom derivatization, data collection, phase calculation and model building, was published by the same group in the Journal of Biochemistry in 1981. The pioneering X-ray analysis of S. platensis Fd at 2.5 Å resolution was a key milestone in structural research on the photosynthetic electron transport chain, informing related and challenging studies on other components of the photosynthetic electron transfer chain.
A Synthesis of Game Theory and Quantitative Genetic Models of Social Evolution
Authors: McGlothlin Joel W , Akçay Erol , Brodie Edmund D , Moore Allen J , Van Cleve Jeremy 
Published: October 2021

AbstractTwo popular approaches for modeling social evolution, evolutionary game theory and quantitative genetics, ask complementary questions but are rarely integrated. Game theory focuses on evolutionary outcomes, with models solving for evolutionarily stable equilibria, whereas quantitative genetics provides insight into evolutionary processes, with models predicting short-term responses to selection. Here we draw parallels between evolutionary game theory and interacting phenotypes theory, which is a quantitative genetic framework for understanding social evolution. First, we show how any evolutionary game may be translated into two quantitative genetic selection gradients, nonsocial and social selection, which may be used to predict evolutionary change from a single round of the game. We show that synergistic fitness effects may alter predicted selection gradients, causing changes in magnitude and sign as the population mean evolves. Second, we show how evolutionary games involving plastic behavioral responses to partners can be modeled using indirect genetic effects, which describe how trait expression changes in response to genes in the social environment. We demonstrate that repeated social interactions in models of reciprocity generate indirect effects and conversely, that estimates of parameters from indirect genetic effect models may be used to predict the evolution of reciprocity. We argue that a pluralistic view incorporating both theoretical approaches will benefit empiricists and theorists studying social evolution. We advocate the measurement of social selection and indirect genetic effects in natural populations to test the predictions from game theory and, in turn, the use of game theory models to aid in the interpretation of quantitative genetic estimates.
Population Dynamics of Wolbachia in the Leafhopper Vector Yamatotettix flavovittatus (Hemiptera: Cicadellidae)
Authors: Wangkeeree Jureemart , Sanit Piyatida , Roddee Jariya , Hanboonsong Yupa 
Published: October 2021

Abstract
Wolbachia (Rickettsiales: Alphaproteobacteria) infections induce abnormalities in the reproductive system and affect various biological traits of the host insects. The density of Wolbachia is one of the major parameters that influence induced phenotypes and interactions with the hosts. Wolbachia occurs naturally in populations of the leafhopper Yamatotettix flavovittatus Matsumura (Hemiptera: Cicadellidae), which transmits phytoplasma that cause white leaf disease in sugarcane. However, the quantity and dynamics of Wolbachia in this leafhopper are not well understood. In the current study, we estimated the number of Wolbachia by absolute quantification of the copy number of wsp, which encodes the outer surface protein, using real-time quantitative polymerase chain reaction (PCR). This investigation was performed using natural populations and laboratory colonies from three lineages of leafhoppers (designated as UD, KK, and SK). There was no significant difference in the number of wsp copies in most of field-collected adults. During the immature developmental stages, there were differences in the dynamics of Wolbachia infection between the UD lineage and the other two lineages. However, the number of wsp copies increased in the early instar and plateaued in the later nymphal instars. Sex had no influence on the number of Wolbachia within the same lineages. The number of Wolbachia was relatively constant during the adult stage in the UD lineage but fluctuated in the other two lineages. In conclusion, the present data provide a framework for exploring the relationship between Wolbachia and the leafhopper and could facilitate future research into management strategies using Wolbachia.
cGAS‒STING signaling and function in metabolism and kidney diseases
Authors: Bai Juli , Liu Feng 
Published: October 2021

Abstract
The cyclic GMP‒AMP synthase (cGAS)‒stimulator of interferon genes (STING) signaling pathway senses the presence of cytosolic DNA and, in turn, triggers downstream signaling to induce the expression of inflammatory and type I interferon genes in immune cells. Whereas the innate immune function of the cGAS‒STING pathway is well studied over the past years, emerging evidence suggests that this signaling pathway may have additional functions beyond innate immune surveillance. Consistent with this notion, dysregulation of the cGAS‒STING signaling pathway in adipocytes, hepatocytes, and renal proximal tubule epithelial cells are associated with metabolic dysfunction, impaired energy homeostasis, and kidney diseases. In this review, we summarize current understanding of the cGAS‒STING pathway in several metabolic diseases such as obesity, insulin resistance, alcoholic and nonalcoholic fatty liver diseases, as well as acute kidney injury and chronic kidney disease. We also review the interaction between the cGAS‒STING pathway and lipid metabolism. Lastly, we discuss potential mechanisms by which cGAS‒STING signaling regulates metabolism and point toward future avenues of research targeting the cGAS‒STING pathway as possible means to treat common metabolic disorders.
Who Gets Referred? A Pilot Study of Risk Stratification and Treatment Referral in Pediatric Headache Using the Pediatric Pain Screening Tool
Authors: Salamon Katherine S , Carlson Megan , Hildenbrand Aimee K 
Published: October 2021

AbstractObjectiveHeadaches are common among youth and are associated with significant negative outcomes. Despite advances in interdisciplinary treatments for youth with chronic pain, research suggests disparities in access to these services.MethodsA total of 186 youth (M = 14.19 years old, 70.8% female) presenting to a neurology appointment at a children’s hospital system were screened using the Pediatric Pain Screening Tool (PPST), a brief, validated measure to identify youth that may benefit from additional pain management services.ResultsTwo-thirds of participants (n = 124, 66.7%) screened as medium or high risk on the PPST. Risk categorization did not vary by patient age or sex. A greater proportion of Hispanic/Latino patients were categorized as low-risk relative to non-Hispanic/Latino patients (55.6% vs. 30.1%), and a somewhat lower proportion of patients of color were categorized as medium-risk relative to White patients (14.0% vs. 30.5%). Three-quarters (n = 94, 75.8%) of patients who were screened as medium or high risk were not referred for any additional pain management services. Referrals did not vary by patient age or ethnicity. While not statistically significant, a lower proportion of males received referrals at both medium (8.3% vs. 17.6%) and high levels of risk (15.8% vs. 34.5%), and a greater proportion of youth of color who screened as medium risk received referrals relative to White youth categorized as medium risk (37.5% vs. 10.3%).ConclusionFuture research should continue to explore factors influencing decision-making regarding referral to specialized pain management services for youth with headache.
Remifentanil protects heart from myocardial ischaemia/reperfusion (I/R) injury via miR-206-3p/TLR4/NF-κB signalling axis
Authors: Zhang Dongyun , Wang Qun , Qiu Xunbin , Chen Yiguan , Yang Xiaoli , Guan Yujian 
Published: October 2021

Abstract

Objectives
Myocardial I/R injury is one of the most serious complications after reperfusion therapy in patients with myocardial infarction. Remifentanil has been found to protect the heart against I/R injury. However, its underlying mechanism remains uncertain in myocardial I/R injury.

Methods
The myocardial I/R injury rat model was established by 30 min of ischaemia followed by 24 h of reperfusion. The animal model was evaluated by the levels of TC, ALT and AST and H&E staining. The binding of miR-206-3p and TLR4 was predicted and verified using TargetScan software, luciferase reporter and RNA pull-down assays. The functional role and mechanism of remifentanil were identified by ultrasonic echocardiography, oxidative stress markers, H&E, Masson and TUNEL staining and western blot.

Key findings
The rat myocardial I/R injury model displayed a significantly high level of TC, ALT, AST, TLR4, p-IκBα and p-p65 and the presence of disorganized cells and inflammatory cell infiltration. The model also showed increased levels of LVEDD, LVESD, MDA, fibrosis and apoptosis and decreased levels of EF, FS, SOD and GSH, which were reversed with remifentanil treatment. Knockdown of miR-206-3p damaged cardiac function and aggravated oxidative stress. miR-206-3p could directly bind to TLR4. TLR4 overexpression destroyed cardiac function, exacerbated oxidative stress, increased levels of p-IκBα and p-p65 and aggravated pathology manifestation affected by remifentanil.

Conclusions
Our results elucidated that remifentanil alleviated myocardial I/R injury by miR-206-3p/TLR4/NF-κB signalling axis.
SABR pre-treatment checks using alanine and nanoDot dosimeters
Authors: Esen Nsikan , Ramachandran Prabhakar , Geso Moshi 
Published: October 2021

ABSTRACTStereotactic Ablative Radiotherapy (SABR) remains one of the preferred treatment techniques for early-stage cancer. It can be extended to more treatment locales involving the sternum, scapula and spine. This work investigates SABR checks using Alanine and nanoDot dosimeter for three treatment sites, including sternum, spine and scapula. Alanine and nanoDot dosimeters’ performances were verified using a 6 MV photon beam before SABR pretreatment verifications. Each dosimeter was placed inside customized designed inserts into a Rod Phantom (in-house phantom) made of Perspex that mimics the human body for a SABR check. Electron Paramagnetic Resonance (EPR) spectrometer, Bruker EleXsys E500 (9.5 GHz) and Microstar (Landauer Inc.) Reader was employed to acquire the irradiated alanine and nanoDot dosimeters’ signal, respectively. Both dosimeters treatment sites are expressed as mean ± standard deviation (SD) of the measured and Eclipse calculated dose Alanine (19.59 ± 0.24, 17.98 ± 0.15, 17.95 ± 0.18) and nanoDot (19.70 ± 0.43, 17.05 ± 0.08, 17.95 ± 0.98) for spine, scapula and sternum, respectively. The percentage difference between alanine and nanoDot dosimeters was within 2% for sternum and scapula but 2.4% for spine cases. These results demonstrate Alanine and nanoDot dosimeters’ potential usefulness for SABR pretreatment quality assurance (QA).
Storage Duration and Red Blood Cell–Derived Microparticles in Packed Red Blood Cells Obtained from Donors with Thalassemia
Authors: Noulsri Egarit , Lerdwana Surada , Palasuwan Duangdao , Palasuwan Attakorn 
Published: October 2021

Abstract

Objective
To address the effects of storage duration on red blood cell (RBC)–derived microparticles (RMPs) in packed RBCs from donors who have thalassemia.

Materials and Methods
Packed RBCs were prepared according to laboratory routine. The quantity of RMPs was determined using FACSCalibur and counting beads.

Results
Across durations of storage, the packed RBCs from donors with thalassemia (n = 28) and healthy volunteers (n = 104) showed average RMPs to be 47,426 (10,139‒127,785) particles/μL vs 49,021 (13,033‒126,749) particles/μL, respectively (P = .63). The peak RMP levels in donors with thalassemia and healthy volunteers, respectively, were shown in products from storage days 34 and 38. Both groups showed a trend toward a positive association between RMP concentration and the duration of storage in packed RBC bags stored under blood bank conditions.

Conclusion
Our results suggest that storage-induced RMP release has similar effects in stored packed RBCs obtained from both donors with thalassemia and healthy volunteers.
Numerical experiments on dynamic evolution of a CME-flare current sheet
Authors: Xie Xiaoyan , Mei Zhixing , Shen Chengcai , Cai Qiangwei , Ye Jing , Reeves Katharine K , Roussev Ilia I , Lin Jun 
Published: October 2021

ABSTRACT
In this paper, we performed magnetohydrodynamics numerical experiments to look into the dynamic behaviour of the current sheet (CS) between the coronal mass ejection (CME) and the associated solar flare, especially the CS oscillation and plasmoid motions in coronal conditions. During the evolution, the disrupting magnetic configuration becomes asymmetric first in the buffer region at the bottom of the CME bubble. The Rayleigh−Taylor instability in the buffer region and the deflected motion of the plasma driven by the termination shock at the bottom of the CME bubble cause the buffer region to oscillate around the y-axis. The local oscillation propagates downwards through the CS, prompting an overall CS oscillation. As the buffer region grows, the oscillation period becomes longer, increasing from about 30 s to about 16 min. Meanwhile, there is another separated oscillation with a period between 0.25 and 1.5 min in the cusp region of the flare generated by velocity shearing. The tearing mode instability yields formations of plasmoids inside the CS. The motions of all the plasmoids observed in the experiment accelerate, which implies that the large-scale CME/flare CS itself in the true eruptive event is filled with the diffusion region according the the standard theory of magnetic reconnection.