bluuebunny/crossref_metadata_2025_split huggingface dataset:
Group Identity as a Prognostic Factor of Mental Health in Preschool Children
Authors: Dyshlova Nataliia V. 
Published: October 2021

Introduction. Victim stance of an individual may arise due to negative social relationships (Chojnacka, 2020) and may be associated with the experience of loneliness (Barbaro, 1999); lack of close friends and rejection by peers; lack of play partner and negative relationships in childhood (Chase, 1999). However, studies investigating the peculiarities of group identity development and the problem of loneliness in preschool children are insufficiently presented in the modern scientific literature. In this article, the phenomenon of victimhood, the experience of acceptance or rejection by a group of peers are considered in the context of preschooler’s mental health genesis.
Purpose. The aim of the study is to determine the features of group identity development in preschool children in kindergartens based on the examples of children and the memories of adults.
Materials and methods: an anonymous survey with open-ended and closed-ended questions was used (n = 247). The participants of the survey were parents of preschoolers attending modern preschool educational institutions (n = 140) and respondents who attended kindergarten as children (n = 107, aged 12 to 55). They were asked to evaluate the organization of groups in kindergarten; the features of interpersonal interaction of children; meeting the needs of children in child-care facilities; the main functions of the kindergarten and their level of trust in educators.
Results: Kindergarten is a second important environment where a child continues to develop his group identity and ego-identity, and not just a place where children stay when parents are at work. Children who have difficulty interacting with their peers often face aggression from peers, experience feelings of loneliness, but not always receive help from their teachers. The majority of respondents (75.7%) answered that the experience of communication in kindergarten influenced their emotional state, self-esteem and communicative competence at school.
Conclusions: The peculiarities of children's group identity in the preschool period are experiences of interest in interaction with a certain group of peers, the desire to be accepted and at the same time hostility towards or lack of interest in other peers. A significant percentage of experienced loneliness among modern children (35.5%) and adults (27.1%) who attended kindergarten more than 30 years ago indicates that this problem has existed for decades and requires more attention from scientists. Absence of play partners and frequent conflicts with peers lead to low self-esteem, fear of communication with children, other toxic experience and, as a consequence, to the risks of victimization in the future
MENTAL HEALTH OF TEACHERS IN UKRAINIAN EDUCATIONAL ORGANIZATIONS
Authors: Dziuba Tetiana , Karamushka Liudmyla , Halushko Lyubov , Zvyagolskaya Iryna , Karamushka Taras 
Published: October 2021

Introduction. Organizational educational environment may contain some destabilizing features (threats), which under certain conditions violate the psychological safety of a teacher and negatively affect his/her mental health and well-being in general. However, the effects of a destabilizing organization of professional activities on mental health and well-being have not been specifically studied, which necessitates appropriate research.
Purpose: to research the indicators of teachers’ mental health in a destabilizing organization of professional activities
Experience in correcting eating disorders in the clinic of mental illness
Authors: Romash Ivan , Romash Iryna , Kukhta Oksana , Dzivak Kateryna , Ahmed Hamna 
Published: October 2021

Introduction: Eating disorders - a class of psychogenic behavioral syndromes associated with eating disorders. The class of these disorders includes anorexia nervosa, bulimia nervosa, compulsive overeating, as well as several other disorders, which are included in the international classification, to section F 50-F 59.
Purpose: The purpose of our study is to trace the dynamics of individual eating disorders in patients with various mental illnesses under the influence of corrective therapy.
Methodology: Were examined 77 patients with various mental diseases who have had eating disorders. The first (I) group included 33 patients. They have added Metoclopramide at a dose of 20 mg/day to the main treatment. The second group (II) - 44 patients who received the drug Ondansetron at a dose of 4 mg/day, respectively. The condition of all patients was assessed according to the following scales: Eating Attitudes Test (EAT-26), 36-Item Short-Form Health Status (SF-36). Body mass index (BMI) was calculated to study the dynamics of weight. All examinations of patients were performed at the beginning and after 14 days of treatment.
Results: According to the EAT-26 scale the average score in the I group was 29 ± 2.8 points, and in the II - 30.1 ± 2.4, respectively. According to the SF-36 questionnaire, the average total score in the I group was - 69.5 ± 1.8 before treatment, 79.5 ± 1.6 - after treatment improved by 12.5% (p <0,05). In group II - 70.8 ± 1.2 and 89.4 ± 1.5, improving by 20.8%, respectively (p <0.05). Under the influence of the therapy, the patients' manifestations of eating behavior decreased, which contributed to the growth of BMI: in group I - by 2.1 ± 1.3 kg, in group II - by 3.5 ± 2.3 kg, which is 1.6 times more.
Conclusions: The drug Ondansetron, intended for the correction of eating disorders, in combination with therapy of the underlying pathology, was likely to be more effective than Metoclopramide hydrochloride, which should be considered in practical psychiatry
International Experience of Legal Regulation of Liability for Insult of Police Officers and Possibilities of Its Use in Ukraine
Authors: Бугайчук К. Л. 
Published: October 2021

The article highlights the problem of the need to strengthen the legal protection of employees of the National Police of Ukraine, who are subjected to illegal encroachments on their honor and dignity while performing their official duties.
It is proved that there are acts concerning insults of police officers and insults or slanders committed against ordinary people in the normative legal acts of the countries of Europe and the former CIS. As a rule, insulting or slandering a law enforcement officer is a crime, while other actions are administrative offenses. Such actions have a qualified composition, which is manifested in the public dissemination of such insults or slander. It is argued that punishment for insulting a police officer is applied only in cases related to the performance of their official duties. Otherwise, the case will be considered in the usual way; the corpus delicti of such a crime may be recognized as qualified if such slander or insult is disseminated through the media or through the Internet.
The current state of legal regulation of legal liability for insult and defamation of police officers in the Republic of Belarus, Kazakhstan, Poland, Germany, France and Spain is analyzed. Based on the study, the propositions to the Law of Ukraine «On the National Police» are formulated that are aimed at regulating the professional guarantees of police activities. It is emphasized that interfering in the activities of a police officer, failure to comply with his legal requirements, obstruction of the exercise of his respective powers, insult or slander against a police officer related to his official activities, insult to the police, as well as any other illegal actions they must be held legally liable for the police officer. The position that the profile law should enshrine in law the state protection of life, health, honor, dignity of a police officer, his professional reputation, members of his family, as well as property belonging to him and his family members from illegal encroachments in connection with the performance of official duties.
Problems of Commissioning of Technical Expert Examination of Documents
Authors: Безсонна Т. Ф. , Зубцова Л. С. 
Published: October 2021

Certain problems that arise during the commissioning of technical expert examination of documents have been studied and analyzed. It has been clarified that they are related to the definition of the type of expert examination, the formulation of the questions to an expert regarding the objects of the examination and the preparation of materials to be examined. It has been noted that the use of special experience is needed for qualitative investigation of criminal offenses. Such experience is used in criminal proceedings in various forms. One of such forms is the commissioning of forensic examinations. One of the most common types of such examinations is technical examination of documents. It solves both diagnostic and identification issues aimed at establishing the fact of a crime, the modus operandi of a crime and the person who committed a crime. Its results are evidence in criminal proceedings.
The ways of solving the problems of commissioning of technical examination of documents have been determined. It has been proved that it is necessary to properly organize the interaction between the initiator of the examination (legal entity or individual) and the expert in order to solve the considered problems. If necessary, one should address the expert institution where the examination is planned to be conducted, and get advice on the possibilities of technical examination of documents depending on the circumstances of the case and the objects of the research. It is advisable to provide expert institutions with modern technical means that should allow us to conduct high-quality and full examination.
It has been offered to improve the methodological materials for the commissioning and conduction of technical examination of documents in accordance with modern scientific and technical capabilities.
Internet of Everything: A Global Solution to Digital world
Authors: Friday Ebole Alpha , Shomope Adewale , Adebowale Adeyemo Gbadebo 
Published: October 2021

The trend of the digital world is the transition from machine-to-machine, machine-to-people, and information technology to human technology with expanded digital to the maturity stage of the internet of everything. The (IoE) is the addition of connectivity and intelligence to every device in order to give them special functions. It embraces four components, namely, people, process, data, and things, and also four technical points of view which are smartness, interconnectivity, big data, and semantic interoperability to deploy the solutions that offer programmability, improved flexibility, and enhanced policy management by the various private and public sectors. The architecture of IoE includes applications, service platform, Internet, gateways, communications, and nodes and attract a security need that will be ubiquitous and able to protect the devices, applications, networks, data, users, and things that make up the Internet of Everything. These systems work collaboratively and smartly with each other and perform the desired task. IoE will be a radical shift to think on how we live, solve the problem, create value, secure environment to keep people, data, processes, and things under digital hamlet. The network capabilities will create new experiences, capabilities, and economic opportunities for individuals, businesses, and governments as a solution to the digital world. The internet of everything is a philosophy of one thing, which is everything.
COVID-19 Pandemi Döneminde Toplu Beslenme Servisi Yapan Farklı Kurumların Menülerinin Değerlendirilmesi
Authors: Sevim Sümeyra , Gümüş Damla , Topal Gülsüm Gizem , Kızıl Mevlüde 
Published: October 2021

Amaç: COVID-19 pandemisi sırasında uygulanan kısıtlamalar eğitim, mesai uygulamaları, sosyal etkinlikler gibi birçok alanda olduğu gibi toplu beslenme hizmetlerini de etkilemiştir. Virüsün yayılımının engellenmesi amacıyla toplu beslenme sistemleri (TBS)’nde çeşitli önlemler geliştirilmiş ve bu önlemler kapsamında bazı kurumlarda masaya servis yerine kumanya menüler servis edilmiştir. Bu çalışma, pandemi döneminde Ankara’da hizmet veren farklı TBS’ye ait menü türlerinin incelenmesi amacıyla gerçekleştirilmiştir.
Gereç ve Yöntem: Çalışmada tek öğün toplu beslenme hizmeti veren üç farklı kurumun (kamu, özel ve yarı özel) iki farklı menü tipi (kumanya ve normal) incelenmiş, menülerin enerji, makro ve mikro besin ögesi içerikleri Türkiye Beslenme Rehberi (TÜBER)’nde önerilen enerji ve besin ögesi gereksinimlerine göre yeterlilik yönünden değerlendirilmiştir. Menülerin besin ögesi örüntüsü Besin Ögesinden Yoğun Besin (NRF) 9.3 ile hesaplanarak incelenmiştir.
Bulgular: Menüler arasında en yüksek NRF9.3 puanı (85.7±18.9) kamu kuruluşunun normal menüsünde, en düşük NRF9.3 puanı (3.3±3.6) ise özel kuruluşun kumanya menüsü (Ö-K)’nde saptanmıştır. En yüksek enerji (1516±284 kkal) sağlayan menü yarı özel kuruluşun kumanya menüsü (Y-K) olurken, ortalama en düşük enerji (927±83 kkal) sağlayan menü Ö-K olmuştur. Ö-K erkekler için enerji; her iki cinsiyet için protein ve lif gereksinmesini karşılamada yetersiz bulunmuştur. Diğer tüm menülerde ise bu besin ögelerinin gereksinimleri karşıladığı tespit edilmiştir.
Sonuç: Bireylerin yeterli ve dengeli beslenmesine katkı sağlamak ve bağışıklık sistemi fonksiyonlarını desteklemek için TBS’de sunulan menülerin, kumanya menüsü gibi farklı servis türleri dahil olmak üzere tüketicilerin enerji ve besin ögesi gereksinmelerini karşılayacak, zengin besin ögesi örüntüsüne ve yüksek diyet kalitesine sahip olması sağlanmalı ve dolayısıyla kurumlarda diyetisyen istihdamının gerekliliği göz ardı edilmemelidir.
A Novel Real-Time Reverse Transcription Loop-Mediated Isothermal Amplification Detection Platform: Application to Diagnosis of COVID-19
Authors: Wang Yi , Wang Xiaoxia , Chen Hai , Han Limei , Wang Licheng , Chen Ting , Li Sha , Li Huan , Li Yuanli , Li Zhengkun , Fu Xiaoying , Chen Shaojin , Xing Mei , Tai Jun , Zhu Xiong 
Published: October 2021

The ongoing Corona virus disease (COVID-19) outbreak has become a huge global health concern. Here, we reported a novel detection platform based on the loop-mediated isothermal amplification (LAMP), termed real-time reverse transcription LAMP (rRT-LAMP) and applied it for the diagnosis of COVID-19 (COVID-19 rRT-LAMP). rRT-LAMP integrates reverse transcription, LAMP amplification, restriction endonuclease cleavage and real-time fluorescence detection into one-pot reaction, and facilitates the diagnosis of COVID-19 at 64°C for only 35 min. The ORF1ab (opening reading frame 1a/b) and NP (nucleoprotein) genes of SARS-CoV-2 were detected for diagnosing COVID-19. The limit of detection (LoD) of COVID-19 rRT-LAMP assay was 14 copies (for each marker) per vessel, and no positive results were obtained from non-SARS-CoV-2 templates. To demonstrate its feasibility, a total of 33 oropharynx swab samples collected from COVID-19 patients also were diagnosed as SARS-CoV-2 infection using COVID-19 rRT-LAMP protocol. No cross-reactivity was yielded from 41 oropharynx swab samples collected from non-COVID-19 patients. These data suggesting that the COVID-19 rRT-LAMP assay is a potential detection tool for the diagnosis of SARS-CoV-2 infection in clinical, field and disease control laboratories, and will be valuable for controlling the COVID-19 epidemic.
ID2 Inhibits Bladder Cancer Progression and Metastasis via PI3K/AKT Signaling Pathway
Authors: Mao Weipu , Wang Keyi , Sun Si , Wu Jianping , Chen Ming , Geng Jiang , Luo Ming 
Published: October 2021

Background: Inhibitors of DNA-binding (ID) proteins are important regulators of cell proliferation and differentiation. The aim of this study was to evaluated the role of ID proteins in bladder cancer (BCa) and related molecular mechanisms.Methods: The TCGA database was analyzed for the expression and clinical significance of ID proteins. The expression of ID2 was determined by qRT-PCR, immunohistochemical staining and western blot. The role of ID2 was determined by CCK-8, colony formation, wound healing, transwell and xenograft tumor assays, and the potential mechanism of ID2 in BCa was investigated by RNA sequencing.Results: ID2 expression was significantly downregulated in TCGA database and clinical samples, and high ID2 expression was associated with low-grade tumor staging and correlated with better overall survival, disease specific survival (DSS) and progress free interval (PFI). In vivo and in vitro experiments showed that knockdown of ID2 promoted proliferation, migration, invasion and metastasis of BCa cells, while overexpression of ID2 significantly inhibited cell proliferation, migration, invasion and metastasis. Mechanistically, ID2 acts as a tumor suppressor through PI3K/AKT signaling pathway to inhibit the progression and metastasis of BCa.Conclusion: Our results suggest that ID2 exerts tumor suppressive effects in BCa through PI3K/AKT signaling pathway, and altered ID2 expression can be used as a biomarker of BCa progression and metastasis.
Site-Specific Protein Modifications by an Engineered Asparaginyl Endopeptidase from Viola canadensis
Authors: Chen Yu , Zhang Dingpeng , Zhang Xiaohong , Wang Zhen , Liu Chuan-Fa , Tam James P. 
Published: October 2021

Asparaginyl endopeptidases (AEPs) or legumains are Asn/Asp (Asx)-specific proteases that break peptide bonds, but also function as peptide asparaginyl ligases (PALs) that make peptide bonds. This ligase activity can be used for site-specific protein modifications in biochemical and biotechnological applications. Although AEPs are common, PALs are rare. We previously proposed ligase activity determinants (LADs) of these enzymes that could determine whether they catalyze formation or breakage of peptide bonds. LADs are key residues forming the S2 and S1′ substrate-binding pockets flanking the S1 active site. Here, we build on the LAD hypothesis with the engineering of ligases from proteases by mutating the S2 and S1′ pockets of VcAEP, an AEP from Viola canadensis. Wild type VcAEP yields &lt;5% cyclic product from a linear substrate at pH 6.5, whereas the single mutants VcAEP-V238A (Vc1a) and VcAEP-Y168A (Vc1b) targeting the S2 and S1′ substrate-binding pockets yielded 34 and 61% cyclic products, respectively. The double mutant VcAEP-V238A/Y168A (Vc1c) targeting both the S2 and S1′ substrate-binding pockets yielded &gt;90% cyclic products. Vc1c had cyclization efficiency of 917,759 M−1s−1, which is one of the fastest rates for ligases yet reported. Vc1c is useful for protein engineering applications, including labeling of DARPins and cell surface MCF-7, as well as producing cyclic protein sfGFP. Together, our work validates the importance of LADs for AEP ligase activity and provides valuable tools for site-specific modification of proteins and biologics.
Associations of Serum S100A12 With Severity and Prognosis in Patients With Community-Acquired Pneumonia: A Prospective Cohort Study
Authors: Jiang Xiao , Huang Chun-Mei , Feng Chun-Mei , Xu Zheng , Fu Lin , Wang Xin-Ming 
Published: October 2021

BackgroundPrevious studies indicated the calcium-binding protein S100A12 to be involved in the pathophysiology of pulmonary inflammatory diseases. However, the role of S100A12 has remained elusive in patients with community-acquired pneumonia (CAP). Therefore, the purpose of this prospective cohort study was to evaluate the association between serum S100A12 with severity and prognosis in CAP patients.MethodsTwo groups with either 239 CAP patients or 239 healthy controls were enrolled in our study. Fasting blood and clinical characteristics were collected. On admission, serum S100A12 was measured using enzyme-linked immunosorbent assay (ELISA).ResultsSerum S100A12 was increased in CAP patients compared to control subjects. Furthermore, serum S100A12 was elevated according to the severity of CAP. Correlative analysis suggested that the level of serum S100A12 was associated with blood routine indices, renal function markers, inflammatory cytokines and other clinical parameters among CAP patients. Additionally, linear and logistical regression analyses indicated that serum S100A12 was positively associated with CAP severity scores in CAP patients. In addition, the association of high serum S100A12 and prognosis was accessed using a follow-up research. Elevated serum S100A12 on admission increased the risk of death and hospital stay in CAP patients during hospitalization.ConclusionsElevated serum S100A12 on admission is positively associated with the severity and adverse prognosis in CAP patients, suggesting that S100A12 may involve in the pathophysiological process of CAP. The titre of serum S100A12 may be used as a biomarker for diagnosis and prognosis among CAP patients.
International Prognostic Index-Based Immune Prognostic Model for Diffuse Large B-Cell Lymphoma
Authors: Mu Shidai , Shi Deyao , Ai Lisha , Fan Fengjuan , Peng Fei , Sun Chunyan , Hu Yu 
Published: October 2021

BackgroundThe International Prognostic Index (IPI) is widely used to discriminate the prognosis of patients with diffuse large B-cell lymphoma (DLBCL). However, there is a significant need to identify novel valuable biomarkers in the context of targeted therapy, such as immune checkpoint blockade (ICB).MethodsGene expression data and clinical DLBCL information were obtained from The Cancer Genome Atlas and Gene Expression Omnibus datasets. A total of 371 immune-related genes in DLBCL patients associated with different IPI risk groups were identified by weighted gene co-expression network analysis, and eight genes were selected to construct an IPI-based immune prognostic model (IPI-IPM). Subsequently, we analyzed the somatic mutation and transcription profiles of the IPI-IPM subgroups as well as the potential clinical response to immune checkpoint blockade (ICB) in IPI-IPM subgroups.ResultsThe IPI-IPM was constructed based on the expression ofCMBL,TLCD3B,SYNDIG1,ESM1,EPHA3,HUNK,PTX3, andIL12A, where high-risk patients had worse overall survival than low-risk patients, consistent with the results in the independent validation cohorts. The comprehensive results showed that high IPI-IPM risk scores were correlated with immune-related signaling pathways, highKMT2DandCD79Bmutation rates, and upregulation of inhibitory immune checkpoints, includingPD-L1,BTLA, andSIGLEC7, indicating a greater potential response to ICB therapy.ConclusionThe IPI-IPM has independent prognostic significance for DLBCL patients, which provides an immunological perspective to elucidate the mechanisms of tumor progression and sheds light on the development of immunotherapy for DLBCL.