bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comparative study of vasodilatation after intra-arterial nicardipine or dantrolene infusion in animal model of cerebral vasospasm
Authors: Lim Jeongwook , Cho Young Dae , Byoun Hyoung Soo 
Published: November 2021

Abstract
Intra-arterial (IA) infusions of calcium channel blockers (CCBs) have been widely applied in treating medically refractory vasospasm. However, surprisingly little is known regarding their vasodilatory duration. This study was undertaken to compare attributes of nicardipine and dantrolene, focusing on efficacy and capacity for sustained vasodilation. In New Zealand white rabbits (N=22), vasospasm was individually provoked through experimentally induced subarachnoid hemorrhage and confirmed via conventional angiography, grouping animals by IA-infused drug (nicardipine vs dantrolene). Controls received normal saline. After chemoangioplasty, follow-up angiography was performed at intervals of 1-3 hours for 6 hours to compare vasospastic and dilated (ie, treated) arterial diameters. Drug efficacy, duration of action, and changes in mean arterial pressure (relative to baseline) were analyzed by group. Compared with controls, effective vasodilation was evident in both nicardipine and dantrolene test groups after IA infusion. Vasodilatory effects of nicardipine peaked at 1 hour, returning to former vasospastic states at 3 hours. In dantrolene recipients, vasodilation endured longer, lasting >6 hours. This outcome suggests that IA dantrolene infused alone or together with a conventional CCB infusion may be a new means of prolonging vasodilatory effect. Further research is needed to assess durations of IA-infused vasodilatory drug based on perfusion status.
TAT-Beclin1 Represses the Carcinogenesis of DUSP4-Positive PTC by Enhancing Autophagy
Authors: Zang Leilei , Song Yanmei , Tian Yanhua , Hu Ning 
Published: November 2021

Abstract
Background DUSP4 is a pro-tumorigenic molecule of papillary thyroid carcinoma (PTC). DUSP4 also exists as an autophagic regulator. Moreover, DUSP4, as a negative regulator of MAPK, can prevent Beclin1 from participating in autophagic response. This study aimed to explore whether TAT-Beclin1, a recombinant protein of Beclin1, could inhibit the tumorigenesis of DUSP4-positive PTC by regulating autophagy. Methods First, we divided PTC cancer tissues into three groups according to DUSP4 expression levels by immunohistochemical analyses, and evaluated the relationship between the expression of autophagic proteins (Beclin1 and LC3II) and DUSP4 expression using Western blotting assays. After overexpression of DUSP4 by lentiviral transduction, the roles of TAT-Beclin1 on DUSP4-overexpressed PTC was detected. Results Our results showed that the expression levels of autophagic proteins increased with the increase of DUSP4 expression in PTC carcinomas. In PTC cells, DUSP4 overexpression-inhibited autophagic activity (including Beclin1 expression, LC3 conversion rate and LC3-puncta formation) and -promoted cell proliferation and migration were reversed by TAT-Beclin1 administration. In vivo assays also showed that DUSP4-overexpressed PTC cells had stronger tumorigenic ability and weaker autophagic activity, which was recovered by TAT-Beclin1 administration. Conclusion TAT-Beclin1, as an autophagic promoter, could repress the carcinogenesis of DUSP4-positive PTC, which implies that the addition of TAT-Beclin1 may be determined through detecting the levels of DUSP4 in the treatment of PTC.
Analysis of the Expression and Prognosis for Leukocyte Immunoglobulin-Like Receptor Subfamily B in Human Liver Cancer
Authors: Fan Jing , Wang Lili , Chen Miao , Zhang Jiakang , Li Jiayan , Song Fangnan , Gu Aidong , Yin Dandan , Yi Yongxiang 
Published: November 2021

Abstract
Background: Leukocyte immunoglobulin-like receptor subfamily B (LILRB), including 5 subtypes, is a group of inhibitory receptors in immune system. The LILRB family is known to be involved in the tumor progression of various cancer types, especially liver cancer. However, the expression patterns and prognostic values of LILRB family members in liver cancer tissues remain unclear.Methods: We used the Oncomine database, GEPIA database, Kaplan–Meier Plotter, Timer, TISIDB and cBioPortal to assess the expression and prognostic value of the LILRB family in liver cancer patients. We also verified the expression of the LILRB family in tumor tissues and tumor-free liver tissues at the protein level by using immunohistochemistry. The STRING website was used to explore the interaction between the LILRB family and their related genes. The DAVID database was used to perform the GO and KEGG analyses. Flow cytometry was used to assess the infiltrated NK cells in liver cancer tissues.Results: Our study revealed that the mRNA expression of LILRB1, LILRB2, LILRB3 and LILRB5 was downregulated, while compared with normal tissues, the mRNA expression of LILRB4 was upregulated in liver cancer tissues. Survival analysis revealed that LILRB2 and LILRB5 mRNA expression levels were significantly associated with OS and DSS and that the mRNA expression of all LILRB family members was significantly correlated with RFS and PFS. Next, we further found that the mRNA expression of all LILRB family members was significantly associated with the infiltration of B cells, CD8+ T cells, CD4+ T cells, macrophages, and neutrophil dendritic cells in liver cancer. Finally, GO and KEGG analyses found that the LILRB family and its related genes were involved in antigen processing and presentation and natural killer cell-mediated cytotoxicity pathways.Conclusions: Our study suggested that LILRB family expression was associated with the prognosis of liver cancer patients and infiltrated immune cells. The LILRB family might be involved in antigen processing and presentation and natural killer cell-mediated cytotoxicity pathways.
The Lockdown Impact Scale for Students (LISS)
Authors: Conroy Ronán M , Fitzgerald Karen 
Published: November 2021

Abstract
Background: The Covid-19 pandemic has resulted in many student populations learning online in lockdown. While the mental health consequences of lockdown are increasingly understood, the core features of ‘cabin fever’ are poorly described. Methods: We conducted a questionnaire survey of 649 undergraduate medicine and health sciences students. Item content was developed based on current literature and input from student representatives. Results: Mokken scaling identified seven questions that together formed a strongly unidimensional scale which comprised two domains : social isolation/cabin fever and demotivation / demoralisation. Scale scores were significantly associated with depression, self-rated mental health, impaired study efficacy and doomscrolling. Conclusions: The adverse effects of lockdown on student wellbeing appear to be driven to an important extent by an experience of isolation and demotivation that correspond to narrative descriptions of cabin fever. In the foreseeable event of future pandemics, these experiences are a promising target for health promotion in students studying in lockdown.
The Epidermal Microbiome Within An Aggregation of Leopard Sharks (Triakis Semifasciata) Has Taxonomic Flexibility With Gene Functional Stability Across Three Time-Points
Authors: Doane Michael , Johnson Colton , Johri Shaili , Kerr Emma N. , Morris Megan M. , Desantiago Ric , Turnlund Abigail C. , Goodman Asha , Mora Maria , Lima Laís Farias Oliveira , Nosal Andrew P. , Dinsdale Elizabeth A. 
Published: November 2021

Abstract
Background: The epidermis of Chondrichthyan fishes consists of dermal denticles with production of minimal but protein rich mucus that influence the attachment and biofilm development of microbes, facilitating a unique epidermal microbiome. Here, we use metagenomics to provide the taxonomic and functional characterization of the epidermal microbiome of the Triakis semifasciata (leopard shark) across three time-points to identify links between microbial groups and host metabolism. Our aims include 1) describing the variation of microbiome taxa over time and identify those members which are recurrent (present across all time-points, 2) investigating the relationship between the recurrent and flexible taxa (those which are not found consistently across time-points, 3) describing the functional compositions of the microbiome which may suggest links with the host metabolism; and 4) identifying whether the metabolisms are share across microbial genera or found in specific taxa. Results: Microbial members of the microbiome showed high similarity between all individuals (average similarity: 82.74) with relative abundance of those members varying across years, suggesting flexibility of taxa in the microbiome. One hundred and eighty-eight genera were identified as recurrent, including Pseudomonas, Erythrobacter, Alcanivorax, Marinobacter and Sphingopxis being consistently abundance across time-points, while Limnobacter and Xyella exhibited switching patterns with high relative abundance in 2013, Sphingobium and Sphingomona in 2015, and Altermonas, Leeuwenhoekiella, Gramella and Maribacter in 2017. Of the 188 genera identified as recurrent, the top 19 relative abundant genera forming three recurrent groups. The microbiome also displayed high functional similarity between individuals (average similarity: 97.65) with gene function composition being consistent across time-points. Conclusion: These results show that while presence of microbial genera exhibit consistency across time-points, their abundances do fluctuate. Functions however remain stable across time points; thus, we suggest the leopard shark microbiomes exhibit functional redundancy. We hypothesize this may be the result of the host’s epidermal attributes structuring the microbiome. In addition, we show the co-existence of many microbial genera that carry genes which may enable the microbes to use the nutrients provided by the elasmobranch’s metabolism.
Climate Change Impact On Flood Runoff Over Lake Baikal Catchment
Authors: Grigorev Vadim Yurevich , Kharlamov Maxim A. , Semenova Natalia K. , Chalov Sergey R. , Sazonov Alexey A. 
Published: November 2021

Abstract
Water level and distribution of dissolved and suspended matter of Lake Baikal are strongly affected by river inflow during rain-driven floods. This study analyses river flow changes at 44 streamflow gauges and related precipitation, evaporation, potential evaporation and soil moisture obtained from ERA5-Land dataset. Based on Sen-Slope trend estimator, Mann–Kendall non-parametric test, and using dominant analyses we estimated influence of meteorological parameters on river flow during 1979-2019. Using ridge-regression we found significant relationships between precipitation elasticity of river flow and catchments features. Half of the gauges in eastern part of Selenga river basin showed a significant decreasing trend of average and maximum river flow (up to -2.9%/year). No changes in central volume date of flood flow have been found. A reduction in rainfall amounts explains more than 60% of runoff decline. Decrease in evaporation is observed where precipitation decrease is 0.8%/y or more. Catchments where the precipitation trends are not as substantial are associated with increasing evaporation as a result of the increase of potential evaporation. Negative trends of precipitation are accompanied by negative trends of soil moisture. Finally, the study reveals sensitivity of the catchments with steep slopes in humid area to precipitation change.
Set-Valued Risk Statistics With The Time Value of Money
Authors: Sun Fei , Fan Xiaozhi , Liu Weitao 
Published: November 2021

Abstract
The time value of money is a critical factor not only in risk analysis, but also in insurance and financial applications. In this paper, we consider a special class of set-valued risk statistics from the perspective of time value of money. This new risk statistic can be uesd for the quantification of portfolio risk. By further developing the properties related to these risk statistics, we are able to derive representation results for such risk.
Assessment and Appliction of Whole-Herd-Sampling, qPCR-Based-Testing, and Precision-Removal Method in Eliminating ASFv in Four Large Swine Herds in China
Authors: Li Xiaowen , Wu Weisheng , Li Peng , Li Junxian , Gao Wenchao , Yu Jincheng , Fan Mingyu , Kang Lixiang , Wang Yunzhou , Yuan Guiqiang , Ren Jing , Yan Zhong , Zhu Liqiang , Li Jintao , Zhang Xiaoyang , Shao Zhenwen , Liu Qingyuan , Zheng Weiwei , Yu Qiannan , Fan Shiran , Huang Yakuan , Chen Jing , Deen John , Yan Zhichun 
Published: November 2021

Abstract
Since the first report of ASFV in China in 2018, conventional whole herd depopulation method to control ASF has proved unwieldly because of high production intensity and complex trade network. To provide an alternative to conventional methods, we evaluated the feasibility of implementating an extensive sampling method and qPCR tests to determine the status of ASFV in herds, with a rapid response to identified outbreaks. By assessing and applying these methods without whole herd depopulation, we successfully controlled ASF and eliminated the virus from 4 large swine herds. The time to negative herd was 19, 28, 14, and 1 days in farms 1-4 respectively. Retention rates of pigs of farm 1 to farm 4 was 69.7%, 65%, 99.4% and 99.72% respectively. We anticipated that this innovative method would replace the conventional stamping out one and greatly facilitate the control and eradication of ASFV in China and worldwide.
Spatial Spillover Effect and Action Path of Electricity Consumption Driven by China’s Financial Development based on Global Co-integration
Authors: Xu Jianjun , Ma Xuejiao , Xu Xiaoqing 
Published: November 2021

Abstract
Although studies on the influencing factors of electricity consumption are rich, the focus on the relationship between financial development and electricity consumption is scarce due to the characteristics of financial sector. In fact, the financial development cannot only increase electricity consumption, but also have the spatial spillover effects. Based on the global spatial modelling techniques, the long-term and short-term relationship between financial development and electricity consumption is examined, and the intermediary effect of financial development on electricity consumption through economic growth, urbanization and industrial structure optimization is also verified. Results show that there is a global co-integration relationship between financial development, economic growth, urbanization, industrial structure optimization and China's electricity consumption, rather than a local co-integration relationship. When the short-term change of electricity consumption deviates from the equilibrium state, the global error correction mechanism can promote the unbalanced system to return to equilibrium from time and spatial dimension. This study not only confirms the spatial spillover effects, but also heterogeneous influences of financial development on electricity consumption, which provides new evidence to make relevant policies.
Design and Experimental Study of the Rolling Enhanced Acoustic System for Gear Tooth Surface
Authors: Jiang Yan , Zhao Bo 
Published: November 2021

Abstract
Parameters of gear flanks, such as roughness, hardness, and residual stress, impact their working performance. Therefore, it is essential to optimize these parameters for increasing gears’ life. Thus, this study combined the meshing theory and the ultrasonic surface rolling technology, and presented an innovative design to realize ultrasonic burnishing. First，the dimensions of the ultrasonic burnishing device with two nodes integrated with a gear at the bottom were calculated using frequency equations by a numerical method. Second the correctness of the calculating results was verified by simulations and experiments. Third, the influence of the ratio between the width and diameter of the gear on the acoustic system was studied. Finally, experimental platform of the rolling enhanced acoustic system for gear tooth surface is built successfully. A series of experiments were conducted, and the results showed that the roughness decreased and the hardness and the residual stress improved. Therefore, this design provided a new theory and technology to machine complicated surfaces such as gear flanks.
Physiochemical effect on the microgroove microstructure in microcutting of pure copper
Authors: Zhang Jiayi 
Published: November 2021

Abstract
The physiochemical effect on the microcutting of pure copper is studied through characterization on the cross-sectioned microgroove. An obvious reduction in cutting force and thrust force can be obtained with the application of ink surfactant. The surface roughness of microgroove with physiochemical effect is 12 nm, and that without physiochemical effect is 17 nm. The average grain size of the ink-affected sample is 67.9 µm within the microgroove zone, and that of the ink-free sample is 48.3 µm within the microgroove zone, moreover, the grain size of ink-free microgroove near the microgroove surface is larger than that far away from the microgroove surface. Additionally, the grain orientations of ink-affected cross-sectioned surface present anisotropy, while that of ink-free cross-sectioned surface are towards {101} direction. Based on the calculation and analysis of geometrically necessary dislocation, it can be inferred that the induced stress and temperature in the sample with physiochemical effect are higher than that without physiochemical effect, which can provide enough driving energy for recrystallization.
PPP2R5D Promotes Hepatitis C Virus Infection Through Binding to NS5B Protein
Authors: Anwar Muhammad Ikram , Li Ni , Zhou Qing , Chen Mingxiao , Hu Chengguang , Wu Tao , Chen Haihang , Li Yi-Ping , Zhou Yuanping 
Published: November 2021

Abstract
Background: Hepatitis C virus (HCV) is an important human pathogen causing chronic hepatitis C, end-stage liver diseases, and hepatocellular carcinoma. The development of infectious HCV cell culture systems primarily relied on the replication enhancement effect of adaptive mutations. Although the mode of action may vary, those adaptive mutations could direct the study of virus-host interactions required for efficient virus infection. We previously identified a substitution D559G in NS5B (RNA dependent RNA polymerase) critical for the replication of HCV genomes. In this study, we set out to study whether D559G-NS5B specifically interacted with some host factors crucial for HCV infection.Methods: Through mass spectrometry analysis of immunoprecipitation mixture of ectopically expressed wild-type and D559G-mutated NS5B, we identified candidate factors showing potential interactions with NS5B and D559G-NS5B. The requirement of selected host factor in HCV infection in vitro was demonstrated by gene knockout, overexpression, virus infection, and co-immunoprecipitation approaches.Results: From the results of immunoprecipitation and mass spectrometry analysis, we selected protein phosphatase 2 regulatory subunit B’delta (PPP2R5D) for further characterization. Co-immunoprecipitation confirmed that both wild-type and D559G NS5B proteins interacted with PPP2R5D, but the interaction between D559G-NS5B and PPP2R5D was more efficient. Silencing of PPP2R5D decreased HCV infection, and knockout of PPP2R5D nearly eliminated HCV infection in Huh7.5 cells. Transient and stably overexpression of PPP2R5D in PPP2R5D-knockout cells restored HCV infection to a level close to that seen for wild-type Huh7.5 cells. Conclusions: PPP2R5D is required for HCV infection in cultured hepatoma cells, and PPP2R5D may function through binding to HCV NS5B. The underlying mechanism of PPP2R5D in the complete HCV life cycle requires further investigation.
Antimicrobial Effect of Geophila Obvallata (Schumach) Didr. Leaf Extracts Against Some Medically Important Bacteria and Fungi
Authors: Iserhienrhien Lucky Osafanme , Paulinus Okolie Ngozi , Mudiaga Etaware Peter 
Published: November 2021

Abstract
Background: The increase in synthetic drug resistance by pathogenic microbes has led to the development of plant-based antimicrobial drugs that are more reliable and non-lethal to human health at increased dosage. Methods: The antibacterial and antifungal potential of Geophila obvallata extracts were tested on clinical isolates (Staphylococcus aureus, Klebsiella pneumoniae, Pseudomonas aeruginosa, Escherichia coli, Bacillus subtilis, Streptococcus pyogenes, Cryptococcus neoformans, Candida albicans and Aspergillus fumigatus) using standard techniques. Results: The zones of inhibition were shown to increase with increasing concentrations of the extracts. Inhibition was higher in Gram positive bacteria (9.10 to 31.00mm in 40mg/mL concentration) than Gram negative bacteria (3.50 to 27.00mm in 40mg/mL concentration), while the fungal isolates had the least zones of inhibition (2.83 to 25.00mm in 40mg/ml concentration). The minimum inhibitory concentrations (MIC) were lowest in the methanol extract than aqueous extract. Simillarly, MIC for bacteria (Bacillus subtilis) and fungi (Aspergillus fumigatus) were 0.3 and 2.0mg/mL respectively. Methanol extract had higher antibacterial and antifungal effect than aqueous extract. Ciprofloxacin, used as control for bacteria had the highest inhibitory activity (33.67mm) when compared to that of the highest concentration of plant extracts administered. Also, ketoconazole gave the highest zones of inhibition (32.33mm) on the fungi isolates compared to those of the extracts. The performance of the methanol extract of 40mg/mL of Geophila obvallata in the inhibition of Bacillus subtilis was not significantly different from that of Ciprofloxacin. Conclusion: The findings in this study therefore validate the antimicrobial effect of Geophila obvallata leaf extracts as well as its possible application in medicine.
Operation Field Contamination During Intraoperative Fluoroscopy
Authors: Li Xiaodong , Yang Fei , Tang Jian , Dai Jingjing , Wang Xiaoqing 
Published: November 2021

Abstract
Background: intraoperative C-arm fluoroscopy is commonly performed during traumatic orthopedic surgeries. The C-arm sterile drape is often used in cases of contamination of the operative field following postoperative infection. The aim of the present study was to investigate operation field contamination during traumatic orthopedic surgeries and evaluate the factors, especially intraoperative fluoroscopy, which affect operation field contamination. Methods: sterile 5% sheep blood Columbia agar plates were used to simulate the operation field. The C-arm was moved over the operation field in different grade clean operating rooms, simulating intraoperative fluoroscopy. The agar plates were then incubated and assessed for bacterial colony growth. Results: our results showed significant differences between the 3rd grade clean operating room and the 2nd or 1st grade clean operating rooms in the risk of operation field contamination. Nevertheless, there were no significant differences in the operation field contamination between the C-arm drape group and the control group. Conclusions: we conclude that C-arm equipment can be used without the drape during orthopedic surgeries to avoid contact with the operation field.