bluuebunny/crossref_metadata_2025_split huggingface dataset:
A novel Penicillium sumatraense isolate reveals an arsenal of degrading enzymes exploitable in algal bio-refinery processes
Authors: Giovannoni M. , Larini I. , Scafati V. , Scortica A. , Compri M. , Pontiggia D. , Zapparoli G. , Vitulo N. , Benedetti M. , Mattei B. 
Published: September 2021

AbstractBackgroundMicroalgae are coming to the spotlight due to their potential applications in a wide number of fields ranging from the biofuel to the pharmaceutical sector. However, several factors such as low productivity, expensive harvesting procedures and difficult metabolite extractability limit their full utilization at industrial scale. Similarly to the successful employment of enzymatic arsenals from lignocellulolytic fungi to convert lignocellulose into fermentable sugars for bioethanol production, specific algalytic formulations could be used to improve the extractability of lipids from microalgae to produce biodiesel. Currently, the research areas related to algivorous organisms, algal saprophytes and the enzymes responsible for the hydrolysis of algal cell wall are still little explored.ResultsHere, an algal trap method for capturing actively growing microorganisms was successfully used to isolate a filamentous fungus, that was identified by whole-genome sequencing, assembly and annotation as a novelPenicilliumsumatraenseisolate. The fungus, classified asP.sumatraenseAQ67100, was able to assimilate heat-killedChlorellavulgariscells by an enzymatic arsenal composed of proteases such as dipeptidyl- and amino-peptidases, β-1,3-glucanases and glycosidases including α- and β-glucosidases, β-glucuronidase, α-mannosidases and β-galactosidases. The treatment ofC.vulgariswith the filtrate fromP.sumatraenseAQ67100 increased the release of chlorophylls and lipids from the algal cells by 42.6 and 48.9%, respectively.ConclusionsThe improved lipid extractability fromC.vulgarisbiomass treated with the fungal filtrate highlighted the potential of algal saprophytes in the bioprocessing of microalgae, posing the basis for the sustainable transformation of algal metabolites into biofuel-related compounds.
Is comprehensiveness critical? Comparing short and long format cognitive assessments in preclinical Alzheimer disease
Authors: Hassenstab Jason , Nicosia Jessica , LaRose Megan , Aschenbrenner Andrew J. , Gordon Brian A. , Benzinger Tammie L. S. , Xiong Chengjie , Morris John C. 
Published: September 2021

Abstract
Background
Comprehensive testing of cognitive functioning is standard practice in studies of Alzheimer disease (AD). Short-form tests like the Montreal Cognitive Assessment (MoCA) use a “sampling” of measures, administering key items in a shortened format to efficiently assess cognition while reducing time requirements, participant burden, and administrative costs. We compared the MoCA to a commonly used long-form cognitive battery in predicting AD symptom onset and sensitivity to AD neuroimaging biomarkers.

Methods
Survival, area under the receiver operating characteristic (ROC) curve (AUC), and multiple regression analyses compared the MoCA and long-form measures in predicting time to symptom onset in cognitively normal older adults (n = 6230) from the National Alzheimer’s Coordinating Center (NACC) cohort who had, on average, 2.3 ± 1.2 annual assessments. Multiple regression models in a separate sample (n = 416) from the Charles F. and Joanne Knight Alzheimer Disease Research Center (Knight ADRC) compared the sensitivity of the MoCA and long-form measures to neuroimaging biomarkers including amyloid PET, tau PET, and cortical thickness.

Results
Hazard ratios suggested that both the MoCA and the long-form measures are similarly and modestly efficacious in predicting symptomatic conversion, although model comparison analyses indicated that the long-form measures slightly outperformed the MoCA (HRs > 1.57). AUC analyses indicated no difference between the measures in predicting conversion (DeLong’s test, Z = 1.48, p = 0.13). Sensitivity to AD neuroimaging biomarkers was similar for the two measures though there were only modest associations with tau PET (rs = − 0.13, ps < 0.02) and cortical thickness in cognitively normal participants (rs = 0.15–0.16, ps < 0.007).

Conclusions
Both test formats showed weak associations with symptom onset, AUC analyses indicated low diagnostic accuracy, and biomarker correlations were modest in cognitively normal participants. Alternative assessment approaches are needed to improve how clinicians and researchers monitor cognitive changes and disease progression prior to symptom onset.
Late presentation of a cauda equina lesion with a vulval abscess: a case report
Authors: Ekanayake Chanil Deshan , Weerasekera Deepal , Dissanayake Dilini , Wickramarachchi Ranga , Pushpakumara Saman , Govindapala Dumitha 
Published: September 2021

Abstract
Background
Cauda equina syndrome is a rare clinical condition that requires prompt diagnosis and timely surgical decompression with postoperative rehabilitation to prevent devastating complications.

Case presentation
A 55-year-old Sinhalese woman presented with a vulval abscess, with a history of involuntary leakage of urine for the last 7 years. Her sexual activity has been compromised due to coital incontinence, and she had also been treated for recurrent urinary tract infections during the last 7 years. On examination, a distended bladder was found. Neurological examination revealed a saddle sensory loss of S2–S4 dermatomes. There was no sensory loss over the lower limbs. Bladder sensation was absent, but there was some degree of anal sphincter tone. Motor functions and reflexes were normal in the limbs. Magnetic resonance imaging revealed L5–S1 spondylolisthesis. Ultrasound imaging confirmed the finding of a distended bladder, in addition to bilateral hydroureters with hydronephrosis. An incision and drainage with concomitant intravenous antibiotics were started for the vulval abscess. An indwelling catheter was placed to decompress the bladder and to reduce vulval excoriations due to urine. Bilateral ureteric stenting was performed later for persistent hydronephrosis and hydroureter despite an empty bladder.

Conclusion
This is a tragic case that illustrates the devastating long-term sequelae that ensues if cauda equina syndrome is left undiagnosed. It reiterates the importance of prompt referral and surgical decompression.
Critical iron deficiency anemia with record low hemoglobin: a case report
Authors: Chai Audrey L. , Huang Owen Y. , Rakočević Rastko , Chung Peter 
Published: September 2021

Abstract
Background
Anemia is a serious global health problem that affects individuals of all ages but particularly women of reproductive age. Iron deficiency anemia is one of the most common causes of anemia seen in women, with menstruation being one of the leading causes. Excessive, prolonged, and irregular uterine bleeding, also known as menometrorrhagia, can lead to severe anemia. In this case report, we present a case of a premenopausal woman with menometrorrhagia leading to severe iron deficiency anemia with record low hemoglobin.

Case presentation
A 42-year-old Hispanic woman with no known past medical history presented with a chief complaint of increasing fatigue and dizziness for 2 weeks. Initial vitals revealed temperature of 36.1 °C, blood pressure 107/47 mmHg, heart rate 87 beats/minute, respiratory rate 17 breaths/minute, and oxygen saturation 100% on room air. She was fully alert and oriented without any neurological deficits. Physical examination was otherwise notable for findings typical of anemia, including: marked pallor with pale mucous membranes and conjunctiva, a systolic flow murmur, and koilonychia of her fingernails. Her initial laboratory results showed a critically low hemoglobin of 1.4 g/dL and severe iron deficiency. After further diagnostic workup, her profound anemia was likely attributed to a long history of menometrorrhagia, and her remarkably stable presentation was due to impressive, years-long compensation. Over the course of her hospital stay, she received blood transfusions and intravenous iron repletion. Her symptoms of fatigue and dizziness resolved by the end of her hospital course, and she returned to her baseline ambulatory and activity level upon discharge.

Conclusions
Critically low hemoglobin levels are typically associated with significant symptoms, physical examination findings, and hemodynamic instability. To our knowledge, this is the lowest recorded hemoglobin in a hemodynamically stable patient not requiring cardiac or supplemental oxygen support.
Managing the economic sustainability of the Belt and Road Initiative by applying Pragmatic Identity Matching PrIM
Authors: Erbach Markus 
Published: September 2021

AbstractAchieving and managing economic sustainability is one of the crucial tasks for our globalized world. One of the most ambitious economic projects known to humankind claims to stand for long-term global economic sustainability including ecological, social, and cultural aspects: the Belt and Road Initiative (BRI), in which China invites the world to join its vision of a “peaceful cooperation for the wealth and cultural exchange of all nations” (Xi Jinping 2013 in his famous speech Promote People-to-People Friendship an Create a Better Future). This invitation led to a wide range of responses, from fundamental rejection to supportive participation. Matching the participants in this megaproject—their contributions, particular prerequisites, and development interests—requires a holistic participative planning approach with solutions tailored specifically to the participating partners. This article shows how Pragmatic Identity Matching (PrIM), a scalable integration framework, can be used to meet this requirement. PrIM provides an identity-oriented infrastructure for aligned planning, implementation and communications, acknowledging, and embracing participants from different cultural backgrounds such as Asian, Arabian, African, Russian, and European cultures. As a structural-scientific approach that synthesizes elemental semiotic thinking and research in psychology and the neurosciences, PrIM provides a meta-planning structure beyond any value-driven positions and perspectives, one that imparts equivalence to information. PrIM can help the BRI avoid a spiral of non-coordinated activities, thereby preventing loss of investment. The formation of a transdisciplinary BRI Management Academy that uses PrIM would help create the necessary infrastructure for a successful and transparent implementation of the BRI.
Simulation-based validation of activity logger data for animal behavior studies
Authors: Chen Jiawei , Brown Geoffrey , Fudickar Adam 
Published: September 2021

AbstractBio-loggers are widely used for studying the movement and behavior of animals. However, some sensors provide more data than is practical to store given experiment or bio-logger design constraints. One approach for overcoming this limitation is to utilize data collection strategies, such as non-continuous recording or data summarization that may record data more efficiently, but need to be validated for correctness. In this paper we address two fundamental questions—how can researchers determine suitable parameters and behaviors for bio-logger sensors, and how do they validate their choices? We present a methodology that uses software-based simulation of bio-loggers to validate various data collection strategies using recorded data and synchronized, annotated video. The use of simulation allows for fast and repeatable tests, which facilitates the validation of data collection methods as well as the configuration of bio-loggers in preparation for experiments. We demonstrate this methodology using accelerometer loggers for recording the activity of the small songbird Junco hyemalis hyemalis.
Regularity in distribution, and control, of pests in the hall of mental cultivation, the Forbidden City, Beijing, China
Authors: Zhang Guoqing , Gu An , Wei Le 
Published: September 2021

Abstract
Pests can seriously threaten the safety of organic relics and historic buildings. Every year, the Palace Museum spends a large of money and time preventing and controlling pests, but there is still no a better systematic and targeted method. The Hall of Mental Cultivation is a representative and essential building in the Forbidden City. Through three years of pest monitoring, the species, quantity, and occurrence regularity of the major pests were investigated. During this time, more than ten species of insects were trapped. These included carpet beetles (Anthrenus picturatus hintoni Mroczkowski, 1952 and Thylodrias contractus Motschulsky, 1839), silverfish (Ctenolepisma longicaudata Escherich, 1905), powder-post beetles (Lyctus brunneus Stephens, 1830), booklice (Liposcelis bostrychophila Badonnel, 1931), spider beetles (Ptinus japonicus Reitter, 1877 and Gibbium aequinoctiale Boieldieu, 1865) and the case bearing clothes moths (Tinea pellionella Linnaeus, 1758), among which the Anthrenus picturatus was the primary pests. Abundant resources of organic relics, suitable temperature, humidity, and light environment, and cypress existence are favorable factors for the occurrence of pests, and analysis of these factors is helpful for further integrated pest management (IPM). The period with the most significant number of active pests in the Hall of Mental Cultivation is July and August, and April and May are the rapid growth period. Carpet beetles are the key pests that can affect the overall change trend significantly. It should combine with their living habits, the ideal treatment time and methods, to give them a better control. Fumigation should be done in July and August, while chemical spray should be done referring to the florescence of outdoor trees (March to June), and used of pheromone is reasonable preventative measures.
Measuring the effectiveness of a car seat program in an urban, level one pediatric trauma center
Authors: Budziszewski Ross , Thompson Rochelle , Lucido Thomas , Walker Janelle , Meyer Loreen K. , Arthur L. Grier , Grewal Harsh 
Published: September 2021

Abstract
Background
Motor vehicle collisions (MVCs) are a significant safety issue in the United States. Young children are disproportionally impacted by car accidents and suffer high rates of injuries and mortality. When used properly, car seats have been found to reduce the severity of injuries. However, individuals from low-income areas often do not have access to education or car seats compared to those in suburban or higher income areas. Therefore, the goal of the present study was to measure the effectiveness of a car seat program in an urban, Level I Pediatric Trauma Center on caregiver car seat knowledge.

Methods
Caregivers (N = 200) attended a single, one-hour car seat educational program with a Child Passenger Safety Technician (CPST). The sessions included educational and hands-on components, where caregivers were asked to complete a seven-item pre-post knowledge assessment. For completion of the course, caregivers received a car seat for their child.

Results
A paired t-test revealed that the workshop significantly increased caregiver knowledge from pre-post: t (199) = − 12.56, p < .001; d = 1.27. McNemar’s Chi-Square analyses displayed that caregivers increased in all knowledge categories (p < .001).

Conclusions
While caregivers in urban areas or in low-income areas may have less access to resources, hospital-led car seat courses can increase knowledge of proper car seat usage in these communities. These findings should be used to establish programs in hospitals in areas where these resources are not readily available to caregivers.
Protect Our Kids: a novel program bringing hemorrhage control to schools
Authors: Tobias Joseph , Cunningham Aaron , Krakauer Kelsi , Nacharaju Deepthi , Moss Lori , Galindo Carlos , Roberts Michael , Hamilton Nicholas A. , Olsen Kyle , Emmons Molly , Quackenbush Jim , Schreiber Martin A. , Burns Beech S. , Sheridan David , Hoffman Benjamin , Gallardo Adrienne , Jafri Mubeen A. 
Published: September 2021

Abstract
Background
Following the shooting at Sandy Hook Elementary School, the Hartford Consensus produced the Stop the Bleed program to train bystanders in hemorrhage control. In our region, the police bureau delivers critical incident training to public schools, offering instruction in responding to violent or dangerous situations. Until now, widespread training in hemorrhage control has been lacking. Our group developed, implemented and evaluated a novel program integrating hemorrhage control into critical incident training for school staff in order to blunt the impact of mass casualty events on children.

Methods
The staff of 25 elementary and middle schools attended a 90-minute course incorporating Stop the Bleed into the critical incident training curriculum, delivered on-site by police officers, nurses and doctors over a three-day period. The joint program was named Protect Our Kids. At the conclusion of the course, hemorrhage control kits and educational materials were provided and a four-question survey to assess the quality of training using a ten-point Likert scale was completed by participants and trainers.

Results
One thousand eighteen educators underwent training. A majority were teachers (78.2%), followed by para-educators (5.8%), counselors (4.4%) and principals (2%). Widely covered by local and state media, the Protect Our Kids program was rated as excellent and effective by a majority of trainees and all trainers rated the program as excellent.

Conclusions
Through collaboration between trauma centers, police and school systems, a large-scale training program for hemorrhage control and critical incident response can be effectively delivered to schools.
Assessing improvements in emergency department referrals to a hospital-based violence intervention program
Authors: Watkins Jayda , Scoggins Na’il , Cheaton Brooke M. , Nimmer Mark , Levas Michael N. , Baumer-Mouradian Shannon H. , Melzer-Lange Marlene D. 
Published: September 2021

Abstract
Background
Youth violence is a major public health concern in the United States. Hospital-based Violence Intervention Programs (HVIPs) are integral in connecting youth sustaining interpersonal violence-related injuries to medical, mental health, and social services. At our pediatric emergency department, our baseline referral rate to the established HVIP was 32.5%. From November 2018–2019, we aimed to increase the percent of eligible patients referred to our HVIP from 32.5 to 70% for patients aged 7–18 years who present to our Level 1 emergency department/trauma center with a violent injury.

Methods
For this quality improvement project, we recorded key aspects of the referral process, such as patient eligibility, who placed referrals, and when referrals were placed in relation to the ED admission. Key stakeholders were interviewed to identify specific interventions. Our key interventions were: 1. Educating providers on eligibility requirements. 2. Encouraging nurses to enter consults at the time of admission. 3. Publishing information about program referrals in the weekly nursing newsletter. 4. Updating social workers on eligibility requirements for the HVIP. We used PDSA cycles to inform our project. Our primary outcome measure was the number of eligible patients referred to our HVIP and measures were analyzed using statistical process control charts.

Results
The HVIP-eligible population had the following demographics: 31.1% female and a mean age 14.3 ± 2.7, 82.6% assaults and 17.4% gunshot wounds. From 11/2018 to 11/2019, there were 78 referrals to the HVIP, out of 167 eligible patients. The referral rate improved from 32.5% pre-interventions to 61.1% post-interventions, showing an 88% increase.

Conclusion(s)
We noted an increase in referrals to our HVIP following our interventions that centered on educating, advertising, and encouraging. Future studies will focus on analyzing other aspects of the enrollment process, such as obtaining patient consent.
Pediatric patients with dog bites presenting to US children’s hospitals
Authors: Ramgopal Sriram , Macy Michelle L. 
Published: September 2021

Abstract
Background
To characterize pediatric dog bite injuries presenting to US children’s hospitals and identify factors associated with clinically significant injuries.

Methods
We performed a multicenter observational study from 26 pediatric hospitals between July 1, 2010, and June 30, 2020, including patients ≤ 18 years with dog bites, consolidating together encounters from patients with multiple encounters within 30 days as a single episode of care. We characterized diagnoses and procedures performed in these patients. We used generalized linear mixed models to identify factors associated with a composite outcome that we term clinically significant injuries (defined as admission, operating room charge, sedation, fractures/dislocations, intracranial/eye injury, skin/soft tissue infection, or in-hospital mortality).

Results
68,833 episodes were included (median age 6.6 years [interquartile range 3.5–10.4 years], 55.5% male) from 67,781 patients. We identified 16,502 patients (24.0%) with clinically significant injuries, including 6653 (9.7%) admitted, 5080 (7.4%) managed in the operating room, 11,685 (17.0%) requiring sedation, 493 (0.7%) with a skull fracture, 32 (0.0%) with a fracture in the neck or trunk, 389 (0.6%) with a fracture of the upper limb, 51 (0.1%) with a fracture in the lower limb, 15 (0.0%) with dislocations, 66 (0.1%) with an intracranial injury and 164 (0.2%) with an injury to the eyeball, 3708 (5.4%) with skin/soft tissue infections, and 5 (0.0%) with in-hospital mortality. In multivariable analysis, younger age (0–4 years, 5–9 years, and 10–14 years relative to 15–18 years), weekday injuries, and an income in the second and third quartiles (relative to the lowest quartile) had higher odds of clinically significant injuries. Black patients (relative to White), Hispanic/Latino ethnicity, and private insurance status (relative to public insurance) had lower odds of clinically important injuries. When evaluating individual components within the composite outcome, most followed broader trends.

Conclusion
Dog bites are an important mechanism of injury encountered in children’s hospitals. Using a composite outcome measure, we identified younger, White, non-Hispanic children at higher risk of clinically significant injuries. Findings with respect to race and ethnicity and dog bite injuries warrant further investigation. Results can be used to identify populations for targeted prevention efforts to reduce severe dog bite injuries.
Understanding how retweets influence the behaviors of social networking service users via agent-based simulation
Authors: Yan Yizhou , Toriumi Fujio , Sugawara Toshiharu 
Published: September 2021

AbstractThe retweet is a characteristic mechanism of several social network services/social media, such as Facebook, Twitter, and Weibo. By retweeting tweet, users can share an article with their friends and followers. However, it is not clear how retweets affect the dominant behaviors of users. Therefore, this study investigates the impact of retweets on the behavior of social media users from the perspective of networked game theory, and how the existence of the retweet mechanism in social media promotes or reduces the willingness of users to post and comment on articles. To address these issues, we propose the retweet reward game model and quote tweet reward game model by adding the retweet and quote tweet mechanisms to a relatively simple social networking service model known as the reward game. Subsequently, we conduct simulation-based experiments to understand the influence of retweets on the user behavior on various networks. It is demonstrated that users will be more willing to post new articles with a retweet mechanism, and quote retweets are more beneficial to users, as users can expect to spread their information and their own comments on already posted articles.