bluuebunny/crossref_metadata_2025_split huggingface dataset:
Enhancement of astaxanthin accumulation using black light in Coelastrum and Monoraphidium isolated from Malaysia
Authors: Kaha Marshila , Iwamoto Koji , Yahya Nurul Ashyikin , Suhaimi Noraiza , Sugiura Norio , Hara Hirofumi , Othman Nor’Azizi , Zakaria Zuriati , Suzuki Kengo 
Published: June 2021

AbstractMicroalgae are important microorganisms which produce potentially valuable compounds. Astaxanthin, a group of xanthophyll carotenoids, is one of the most powerful antioxidants mainly found in microalgae, yeasts, and crustaceans. Environmental stresses such as intense light, drought, high salinity, nutrient depletion, and high temperature can induce the accumulation of astaxanthin. Thus, this research aims to investigate the effect of black light, also known as long-wave ultraviolet radiation or UV-A, as a stressor on the accumulation of astaxanthin as well as to screen the antioxidant property in two tropical green algal strains isolated from Malaysia, Coelastrum sp. and Monoraphidium sp. SP03. Monoraphidium sp. SP03 showed a higher growth rate (0.66 day−1) compared to that of Coelastrum sp. (0.22 day−1). Coelastrum sp. showed significantly higher accumulation of astaxanthin in black light (0.999 g mL culture−1) compared to that in control condition (0.185 g mL−1). Similarly, Monoraphidium sp. SP03 showed higher astaxanthin content in black light (0.476 g mL culture−1) compared to that in control condition (0.363 g mL culture−1). Coelastrum sp. showed higher scavenging activity (30.19%) when cultured in black light condition, indicating a correlation between the antioxidant activity and accumulation of astaxanthin. In this study, black light was shown to possess great potential to enhance the production of astaxanthin in microalgae.
Generation of tetracycline-controllable CYP3A4-expressing Caco-2 cells by the piggyBac transposon system
Authors: Ichikawa Moe , Akamine Hiroki , Murata Michika , Ito Sumito , Takayama Kazuo , Mizuguchi Hiroyuki 
Published: June 2021

AbstractCaco-2 cells are widely used as an in vitro intestinal epithelial cell model because they can form a monolayer and predict drug absorption with high accuracy. However, Caco-2 cells hardly express cytochrome P450 (CYP), a drug-metabolizing enzyme. It is known that CYP3A4 is the dominant drug-metabolizing enzyme in human small intestine. In this study, we generated CYP3A4-expressing Caco-2 (CYP3A4-Caco-2) cells and attempted to establish a model that can simultaneously evaluate drug absorption and metabolism. CYP3A4-Caco-2 cells were generated by piggyBac transposon vectors. A tetracycline-controllable CYP3A4 expression cassette (tet-on system) was stably transduced into Caco-2 cells, thus regulating the levels of CYP3A4 expression depending on the doxycycline concentration. The CYP3A4 expression levels in CYP3A4-Caco-2 cells cultured in the presence of doxycycline were similar to or higher than those of adult small intestine. The CYP3A4-Caco-2 cells had enough ability to metabolize midazolam, a substrate of CYP3A4. CYP3A4 overexpression had no negative effects on cell proliferation, barrier function, and P-glycoprotein activity in Caco-2 cells. Thus, we succeeded in establishing Caco-2 cells with CYP3A4 metabolizing activity comparable to in vivo human intestinal tissue. This cell line would be useful in pharmaceutical studies as a model that can simultaneously evaluate drug absorption and metabolism.
Sex-specific impact of diabetes mellitus on left ventricular systolic function and prognosis in heart failure
Authors: Kwak Soongu , Hwang In-Chang , Park Jin Joo , Park Jae-Hyeong , Park Jun-Bean , Cho Goo-Yeong 
Published: June 2021

AbstractWe aimed to investigate the sex differences in associations of diabetes mellitus (DM) with echocardiographic phenotypes and clinical outcomes of heart failure (HF). We studied 4,180 patients admitted for acute HF between 2009 and 2016 (median follow-up, 31.7 months) whose left ventricular global longitudinal strain (LV-GLS) data were available. Patients were compared by sex and DM. Structural equation model (SEM) analysis was performed to evaluate the moderating effects of two causal paths, via ischemic heart disease (IHD) and LV-GLS, linking DM with mortality. Compared to non-diabetic women, diabetic women had significantly lower LV-GLS (11.3% versus 10.1%, p < 0.001), but the difference was attenuated within men (9.7% versus 9.2%, p = 0.014) (p-for-interaction by sex = 0.018). In Cox analyses, DM was an independent predictor for higher mortality in both sexes (women: adjusted hazard ratio [HR] 1.35, 95% confidence interval [CI] 1.15–1.59 versus men: HR 1.24, 95% CI 1.07–1.44, p-for-interaction by sex = 0.699). Restricted cubic spline curves showed that LV-GLS consistently declined, and mortality increased in women with worsening hyperglycemia, but these trends were not evident in men. In SEM analysis, the main driver from DM to mortality differed by sex; men had a stronger effect via IHD than LV-GLS, whereas LV-GLS was the only predominant path in women.
Process optimization and enhancement of pesticide adsorption by porous adsorbents by regression analysis and parametric modelling
Authors: Dehghani Mohammad Hadi , Hassani Amir Hessam , Karri Rama Rao , Younesi Bahareh , Shayeghi Mansoureh , Salari Mehdi , Zarei Ahmad , Yousefi Mahmood , Heidarinejad Zoha 
Published: June 2021

AbstractIn the present study, the adsorptive removal of organophosphate diazinon pesticide using porous pumice adsorbent was experimentally investigated in a batch system, modelled and optimized upon response surface methodology (RSM) and artificial neural network-genetic algorithm (ANN-GA), fitted to isotherm, kinetic and thermodynamic models. The quantification of adsorbent elements was determined using EDX. XRD analysis was utilized to study the crystalline properties of adsorbent. The FT-IR spectra were taken from adsorbent before and after adsorption to study the presence and changes in functional groups. The constituted composition of the adsorbent was determined by XRF. Also, the ionic strength and adsorbent reusability were explored. The influences of operational parameters like pH, initial pesticide concentration, adsorbent dosage and contact time were investigated systematically. ANN-GA and RSM techniques were used to identify the optimal process variables that result in the highest removal. Based on the RSM approach, the optimization conditions for maximum removal efficiency is obtained at pH = 3, adsorbent dosage = 4 g/L, contact time = 30 min, and initial pesticide concentration = 6.2 mg/L. To accurately identify the parameters of nonlinear isotherm and kinetic models, a hybrid evolutionary differential evolution optimization (DEO) is applied. Results indicated that the equilibrium adsorption data were best fitted with Langmuir and Temkin isotherms and kinetic data were well described by pseudo-first and second-order kinetic models. The thermodynamic parameters such as entropy, enthalpy and Gibbs energy were evaluated to study the effect of temperature on pesticide adsorption.
Chronic liver disease not a significant comorbid condition for COVID-19
Authors: Lin Jiahao , Bao Bingting , Khurram Nigar Anjuman , Halsey Kasey , Choi Ji Whae , Wang Lesan , Tran Thi My Linh , Liao Wei-Hua , Feldman Michael D. , Zhang Paul J. , Wu Jing , Bai Harrison X. 
Published: June 2021

AbstractTo explore the role of chronic liver disease (CLD) in COVID-19. A total of 1439 consecutively hospitalized patients with COVID-19 from one large medical center in the United States from March 16, 2020 to April 23, 2020 were retrospectively identified. Clinical characteristics and outcomes were compared between patients with and without CLD. Postmortem examination of liver in 8 critically ill COVID-19 patients was performed. There was no significant difference in the incidence of CLD between critical and non-critical groups (4.1% vs 2.9%, p = 0.259), or COVID-19 related liver injury between patients with and without CLD (65.7% vs 49.7%, p = 0.065). Postmortem examination of liver demonstrated mild liver injury associated central vein outflow obstruction and minimal to moderate portal lymphocytic infiltrate without evidence of CLD. Patients with CLD were not associated with a higher risk of liver injury or critical/fatal outcomes. CLD was not a significant comorbid condition for COVID-19.
Evolutionary history expands the range of signaling interactions in hybrid multikinase networks
Authors: Ortet Philippe , Fochesato Sylvain , Bitbol Anne-Florence , Whitworth David E. , Lalaouna David , Santaella Catherine , Heulin Thierry , Achouak Wafa , Barakat Mohamed 
Published: June 2021

AbstractTwo-component systems (TCSs) are ubiquitous signaling pathways, typically comprising a sensory histidine kinase (HK) and a response regulator, which communicate via intermolecular kinase-to-receiver domain phosphotransfer. Hybrid HKs constitute non-canonical TCS signaling pathways, with transmitter and receiver domains within a single protein communicating via intramolecular phosphotransfer. Here, we report how evolutionary relationships between hybrid HKs can be used as predictors of potential intermolecular and intramolecular interactions (‘phylogenetic promiscuity’). We used domain-swap genes chimeras to investigate the specificity of phosphotransfer within hybrid HKs of the GacS–GacA multikinase network of Pseudomonas brassicacearum. The receiver domain of GacS was replaced with those from nine donor hybrid HKs. Three chimeras with receivers from other hybrid HKs demonstrated correct functioning through complementation of a gacS mutant, which was dependent on strains having a functional gacA. Formation of functional chimeras was predictable on the basis of evolutionary heritage, and raises the possibility that HKs sharing a common ancestor with GacS might remain components of the contemporary GacS network. The results also demonstrate that understanding the evolutionary heritage of signaling domains in sophisticated networks allows their rational rewiring by simple domain transplantation, with implications for the creation of designer networks and inference of functional interactions.
Sleep duration and napping in relation to colorectal and gastric cancer in the MCC-Spain study
Authors: Papantoniou Kyriaki , Castaño-Vinyals Gemma , Espinosa Ana , Turner Michelle C. , Martín-Sánchez Vicente , Casabonne Delphine , Aragonés Nuria , Gómez-Acebo Inés , Ardanaz Eva , Jimenez-Moleon Jose-Juan , Amiano Pilar , Molina-Barceló Ana , Alguacil Juan , Fernández-Tardón Guillermo , Huerta José María , Hernández-Segura Natalia , Perez-Gomez Beatriz , Llorca Javier , Vidán-Alli Juana , Olmedo-Requena Rocıo , Gil Leire , Castañon-López Carmen , Pollan Marina , Kogevinas Manolis , Moreno Victor 
Published: June 2021

AbstractSleep duration is a novel and potentially modifiable risk factor for cancer. We evaluated the association of self-reported sleep duration and daytime napping with odds of colorectal and gastric cancer. We included 2008 incident colorectal cancer cases, 542 gastric cancer cases and 3622 frequency-matched population controls, recruited in the MCC-Spain case–control study (2008–2013). Sleep information, socio-demographic and lifestyle characteristics were obtained through personal interviews. Multivariable adjusted logistic regression models were used to estimate odds ratios (OR) with 95% confidence intervals (CI) for cancer, across categories of sleep duration (≤ 5, 6, 7, 8, ≥ 9 hours/day), daytime napping frequency (naps/week) and duration (minutes/nap). Compared to 7 hours of sleep, long sleep was associated with increased odds of colorectal (OR≥9 hours: 1.59; 95%CI 1.30–1.94) and gastric cancer (OR≥9 hours: 1.95; 1.37–2.76); short sleep was associated with increased odds of gastric cancer (OR≤5 hours: 1.32; 0.93–1.88). Frequent and long daytime naps increased the odds of colorectal (OR6–7 naps/week, ≥30 min: 1.32; 1.14–1.54) and gastric cancer (OR6–7 naps/week, ≥30 min: 1.56; 1.21–2.02). Effects of short sleep and frequent long naps were stronger among participants with night shift-work history. Sleep and circadian disruption may jointly play a role in the etiology of colorectal and gastric cancer.
High-resolution metabolomic biomarkers for lung cancer diagnosis and prognosis
Authors: Qi Shi-ang , Wu Qian , Chen Zhenpu , Zhang Wei , Zhou Yongchun , Mao Kaining , Li Jia , Li Yuanyuan , Chen Jie , Huang Youguang , Huang Yunchao 
Published: June 2021

AbstractLung cancer is the leading cause of human cancer mortality due to the lack of early diagnosis technology. The low-dose computed tomography scan (LDCT) is one of the main techniques to screen cancers. However, LDCT still has a risk of radiation exposure and it is not suitable for the general public. In this study, plasma metabolic profiles of lung cancer were performed using a comprehensive metabolomic method with different liquid chromatography methods coupled with a Q-Exactive high-resolution mass spectrometer. Metabolites with different polarities (amino acids, fatty acids, and acylcarnitines) can be detected and identified as differential metabolites of lung cancer in small volumes of plasma. Logistic regression models were further developed to identify cancer stages and types using those significant biomarkers. Using the Variable Importance in Projection (VIP) and the area under the curve (AUC) scores, we have successfully identified the top 5, 10, and 20 metabolites that can be used to differentiate lung cancer stages and types. The discrimination accuracy and AUC score can be as high as 0.829 and 0.869 using the five most significant metabolites. This study demonstrated that using 5 + metabolites (Palmitic acid, Heptadecanoic acid, 4-Oxoproline, Tridecanoic acid, Ornithine, and etc.) has the potential for early lung cancer screening. This finding is useful for transferring the diagnostic technology onto a point-of-care device for lung cancer diagnosis and prognosis.
Osmotic stress and vesiculation as key mechanisms controlling bacterial sensitivity and resistance to TiO2 nanoparticles
Authors: Pagnout Christophe , Razafitianamaharavo Angelina , Sohm Bénédicte , Caillet Céline , Beaussart Audrey , Delatour Eva , Bihannic Isabelle , Offroy Marc , Duval Jérôme F. L. 
Published: June 2021

AbstractToxicity mechanisms of metal oxide nanoparticles towards bacteria and underlying roles of membrane composition are still debated. Herein, the response of lipopolysaccharide-truncated Escherichia coli K12 mutants to TiO2 nanoparticles (TiO2NPs, exposure in dark) is addressed at the molecular, single cell, and population levels by transcriptomics, fluorescence assays, cell nanomechanics and electrohydrodynamics. We show that outer core-free lipopolysaccharides featuring intact inner core increase cell sensitivity to TiO2NPs. TiO2NPs operate as membrane strippers, which induce osmotic stress, inactivate cell osmoregulation and initiate lipid peroxidation, which ultimately leads to genesis of membrane vesicles. In itself, truncation of lipopolysaccharide inner core triggers membrane permeabilization/depolarization, lipid peroxidation and hypervesiculation. In turn, it favors the regulation of TiO2NP-mediated changes in cell Turgor stress and leads to efficient vesicle-facilitated release of damaged membrane components. Remarkably, vesicles further act as electrostatic baits for TiO2NPs, thereby mitigating TiO2NPs toxicity. Altogether, we highlight antagonistic lipopolysaccharide-dependent bacterial responses to nanoparticles and we show that the destabilized membrane can generate unexpected resistance phenotype.
Arctic open-water periods are projected to lengthen dramatically by 2100
Authors: Crawford Alex , Stroeve Julienne , Smith Abigail , Jahn Alexandra 
Published: June 2021

AbstractThe shrinking of Arctic-wide September sea ice extent is often cited as an indicator of modern climate change; however, the timing of seasonal sea ice retreat/advance and the length of the open-water period are often more relevant to stakeholders working at regional and local scales. Here we highlight changes in regional open-water periods at multiple warming thresholds. We show that, in the latest generation of models from the Coupled Model Intercomparison Project (CMIP6), the open-water period lengthens by 63 days on average with 2 °C of global warming above the 1850-1900 average, and by over 90 days in several Arctic seas. Nearly the entire Arctic, including the Transpolar Sea Route, has at least 3 months of open water per year with 3.5 °C warming, and at least 6 months with 5 °C warming. Model bias compared to satellite data suggests that even such dramatic projections may be conservative.
Task offloading and resource allocation algorithm based on mobile edge computing in Internet of Things environment
Authors: Liu Junwei 
Published: June 2021

AbstractThis paper proposes a task offloading and resource allocation algorithm based on mobile edge computing. Firstly, for the long‐term performance optimization of small base stations, the system model is established according to task arrival characteristics, credit relationship between small base stations, time delay and energy consumption of computing tasks and cable channel congestion. Secondly, the energy consumption deficit queue based on Lyapunov drift penalty technology used for the energy consumption constraint of small base stations in long‐term optimization process. The energy consumption deficit queue is established for each small base station to couple the energy consumption and time of small base stations, so that small base stations can meet the energy consumption constraints in long‐term optimization process. Finally, game theory is introduced to calculate offloading weight by the offloading weight model based on Shapley value. Besides, the offloading weight is calculated equitably according to the return of different tasks. Simulation results on MATLAB platform show that the proposed algorithm can achieve Nash equilibrium after finite iterations. Moreover, its performance on energy consumption, time delay and number of tasks successfully offloaded is better than other comparison strategies.
“Flow Reversal” and Cure in a Case of Giant Intracranial Aneurysm: A Case Report
Authors: Shah Abhidha , Vutha Ravikiran , Doshi Jash , Trivedi Nishit , Goel Atul 
Published: June 2021

AbstractWe describe the case of an 11-year-old girl having a giant anterior circulation aneurysm. The ipsilateral internal carotid artery was entirely blocked and the aneurysm was supplied by posterior circulation. Following a high-flow bypass that connected the external carotid artery to the middle cerebral artery, the giant aneurysm thrombosed spontaneously. We discuss several relatively rare and unique features of the case.
Wound Care for Venous Ulceration
Authors: Schroeppel DeBacker Sarah E. , Bulman Julie C. , Weinstein Jeffrey L. 
Published: June 2021

AbstractVenous leg ulcers (VLUs) affect as many as 20% of patients with advanced chronic venous insufficiency and are associated with significant morbidity and health care costs. VLUs are the most common cause of leg ulcers; however, other etiologies of lower extremity ulcerations should be investigated, most notably arterial insufficiency, to ensure appropriate therapy. Careful clinical examination, standardized documentation, and ultrasound evaluation are needed for diagnosis and treatment success. Reduction of edema and venous hypertension through compression therapy, local wound care, and treatment of venous reflux or obstruction is the foundation of therapy. As key providers in venous disease, interventional radiologists should be aware of current standardized disease classification and scoring systems as well as treatment and wound care guidelines for venous ulcers.
Thirty-Day Unplanned Readmission after Total Hip Arthroplasty at a Community Hospital: Rates, Reasons, and Risk Factors
Authors: Workman Kalain , Folau Christopher K. , Phillips David S. 
Published: June 2021

AbstractUnplanned readmission after total hip arthroplasty (THA) has an increasing prevalence in the United States. Readmissions are a metric for a hospital's quality of care, yet the reasons behind them are mixed. In this changing health-care environment, it is critical for institutions to identify the risk factors for unplanned readmissions following a THA. Retrospective chart review and hospital administrative database query were used to report causes, demographics, and medical comorbidities linked to a 30-day readmission after primary THA at a teaching community hospital system. This study identified 4,459 primary THA procedures, of which 96 (2.2%) were unplanned readmissions. Periprosthetic fracture (20.8%) and periprosthetic infection (17.7%) were the most common causes of readmission. Patients discharged to rehab (odds ratio [OR], 2.44; 95% confidence interval [CI], 1.36–4.37; p = 0.0026) were correlated significantly with the risk of 30-day readmission. Anemia (OR, 2.62; 95% CI, 1.44–4.79; p = 0.0016), obstructive sleep apnea (OR, 2.27; 95% CI, 1.17–4.4; p = 0.0150), and asthma (OR, 2.51; 95% CI, 1.17–5.40; p = 0.0184) were significant independent risk factors. Charlson comorbidity index (p = 0.3634) was not shown to correlate with a quicker readmission within 30 days of discharge. Patients with a history of anemia, obstructive sleep apnea, and asthma should be medically optimized before and after undergoing THA in the community setting. Implementing postsurgical emphasis on discharging patients with home care options rather than transferring them to a rehabilitation facility may prevent 30-day readmissions.
Management of Patients when Superficial Venous Disease Arises from Pelvic Escape Points
Authors: Ahuja Rakesh S. , Garg Tushar , Sudheendra Deepak 
Published: June 2021

AbstractChronic pelvic pain (CPP) is a common condition in women that carries with it significant morbidity. It is commonly seen in patients presenting to obstetrics and gynecology outpatient clinic visits. CPP is a presenting symptom of various pathologies including pelvic varicocele, pelvic adhesions, spastic colon syndrome, uterine fibroids, endometriosis, and psychosomatic disorders. Pelvic congestion syndrome has more recently been termed “pelvic venous insufficiency (PVI)” due to the underlying retrograde flow through incompetent ovarian and pelvic veins that are thought to cause the symptoms of CPP. Pelvic varices can commonly present alongside vulvar, perineal, and lower extremity varices. There are some predictable “escape pathways” for these varices that may present for interventional treatment. This article introduces the reader to current terminology, clinical presentation, diagnosis, and treatment of patients with pelvic varices due to PVI.
Clinical Presentation, Patient Assessment, Anatomy, Pathophysiology, and Imaging of Pelvic Venous Disease
Authors: Maratto Sean , Khilnani Neil M. , Winokur Ronald S. 
Published: June 2021

AbstractPelvic venous disorders (PeVDs) can result in several different clinical presentations, but can be challenging to distinguish from other etiologies of chronic pelvic pain (CPP). Clinical evaluation of CPP patients optimally should be performed in a multidisciplinary fashion and patients who may have PeVD should be referred for consultation with a vascular interventionalist whose evaluation would utilize an imaging workup to search for pelvic varices. Additionally, it is critical to quantify the quality-of-life effects of all CPP to determine the impact on the patient's overall health. Diagnostic imaging, including transabdominal and transvaginal ultrasound, computed tomography, magnetic resonance imaging, and venography, can be utilized to identify pelvic varices, as well as venous reflux and obstruction leading to CPP. The use of the SVP tool is important to classify PeVD patients based on their clinical symptoms, varicose veins, and pathophysiology for precise clinical communication and for reporting clinical research. The goal of this publication is to delineate the clinical presentation, anatomy, pathophysiology, and imaging evaluation of patients with CPP suspected of having PeVD.