bluuebunny/crossref_metadata_2025_split huggingface dataset:
Testing the effect of an integrated-intervention to promote access to sexual and reproductive healthcare and rights among women with disabilities in Ghana: a quasi-experimental study protocol
Authors: Ganle John Kuumuori , Ofori Charlotte , Dery Samuel 
Published: October 2021

Abstract
Background
There is evidence that women with disabilities (WWDs) experience the most difficulty accessing and using sexual and reproductive health and rights (SRHRs) services and information worldwide. However, there are currently no workable interventions to reach WWDs with essential SRHR services. This study aims to test the effect of an integrated health facility and individual-level intervention on access to SRHRs information and services among sexually active WWDs aged 15–49 years in Ghana.

Methods
A quasi-experimental study design with four arms will be implemented in four districts in the Northern region of Ghana to test the effect of three inter-related interventions. The inventions are (1) capacity building in disability-centred SRHRs information and service delivery for healthcare providers, (2) support for WWDs to access disability-unfriendly healthcare infrastructure, and (3) one-on-one regular SRHRs education, information provision, and referral. The first two interventions are at the health-facility level while the third one is at the individual/family level. The first arm of the experiment will expose eligible WWDs to all three interventions. In the second arm, WWDs will be exposed to only the two-health facility-level interventions. The third arm will expose WWDs to only the individual level intervention. The forth arm will constitute the control group. A total of 680 (170 in each arm) sexually active women with physical disability and visual impairments will take part in the study over a period of 12 months. To assess the effect of the interventions on key study outcomes (i.e. awareness about, and use of modern contraceptive, ANC attendance, and skilled delivery among parous women), pre- and post-intervention surveys will be conducted. Difference-in-Difference analysis will be used to examine the effect of each intervention in comparison to the control group, while controlling for confounders. Cost-effectiveness analyses will also be conducted on the three-intervention arms vis a vis changes in key outcome measures to identify which of the three interventions is likely to yield greater impact with lower costs.

Discussion
Lack of access to SRHRs information and services for WWDs is not only a violation of their right to appropriate and quality SRH care but could also undermine efforts to achieve equitable healthcare access as envisaged under SDG 3. This research is expected to generate evidence to inform local health programmes to increase access to SRHRs among WWDs by strengthening local health system capacity to provide disability-sensitive SRHRs services.
Trial registration Name of the registry: Pan African Clinical Trials Registry (PACTR). Trial ID: 14591. Date of registration: 02/01/2020. URL of trial registry record: https://pactr.samrc.ac.za/Researcher/TrialRegister.aspx?TrialID=14591
Granulocyte-macrophage colony-stimulating factor and tumor necrosis factor-α in combination is a useful diagnostic biomarker to distinguish familial Mediterranean fever from sepsis
Authors: Koga Tomohiro , Furukawa Kaori , Migita Kiyoshi , Morimoto Shimpei , Shimizu Toshimasa , Fukui Shoichi , Umeda Masataka , Endo Yushiro , Sumiyoshi Remi , Kawashiri Shin-ya , Iwamoto Naoki , Ichinose Kunihiro , Tamai Mami , Origuchi Tomoki , Maeda Takahiro , Yachie Akihiro , Kawakami Atsushi 
Published: October 2021

Abstract
Objective
To identify potential biomarkers to distinguish familial Mediterranean fever (FMF) from sepsis.

Method
We recruited 28 patients diagnosed with typical FMF (according to the Tel Hashomer criteria), 22 patients with sepsis, and 118 age-matched controls. Serum levels of 40 cytokines were analyzed using multi-suspension cytokine array. We performed a cluster analysis of each cytokine in the FMF and sepsis groups in order to identify specific molecular networks. Multivariate classification (random forest analysis) and logistic regression analysis were used to rank the cytokines by importance and determine specific biomarkers for distinguishing FMF from sepsis.

Results
Fifteen of the 40 cytokines were found to be suitable for further analysis. Levels of serum granulocyte-macrophage colony-stimulating factor (GM-CSF), fibroblast growth factor 2, vascular endothelial growth factor, macrophage inflammatory protein-1b, and interleukin-17 were significantly elevated, whereas tumor necrosis factor-α (TNF-α) was significantly lower in patients with FMF compared with those with sepsis. Cytokine clustering patterns differed between the two groups. Multivariate classification followed by logistic regression analysis revealed that measurement of both GM-CSF and TNF-α could distinguish FMF from sepsis with high accuracy (cut-off values for GM-CSF = 8.3 pg/mL; TNF-α = 16.3 pg/mL; sensitivity, 92.9%; specificity, 94.4%; accuracy, 93.4%).

Conclusion
Determination of GM-CSF and TNF-α levels in combination may represent a biomarker for the differential diagnosis of FMF from sepsis, based on measurement of multiple cytokines.
Generalized fixed point results of rational type contractions in partially ordered metric spaces
Authors: Seshagiri Rao N. , Kalyani K. 
Published: October 2021

Abstract
Objectives
We investigated the existence and uniqueness of a fixed point for the mapping satisfying generalized rational type contraction conditions in metric space endowed with partial order. Suitable examples are presented to justify the results obtained.

Result
Some new fixed point results have been obtained for a mapping fulfilling generalized contractions. The uniqueness of the fixed point is also the part of the study based on an ordered relation. One example is given for a result which is not valid in the usual metric space.
Label-free separation of neuroblastoma patient-derived xenograft (PDX) cells from hematopoietic progenitor cell products by acoustophoresis
Authors: Olm Franziska , Panse Lena , Dykes Josefina H. , Bexell Daniel , Laurell Thomas , Scheding Stefan 
Published: October 2021

Abstract
Background
Graft-contaminating tumor cells correlate with inferior outcome in high-risk neuroblastoma patients undergoing hematopoietic stem cell transplantation and can contribute to relapse. Motivated by the potential therapeutic benefit of tumor cell removal as well as the high prognostic and diagnostic value of isolated circulating tumor cells from stem cell grafts, we established a label-free acoustophoresis-based microfluidic technology for neuroblastoma enrichment and removal from peripheral blood progenitor cell (PBPC) products.

Methods
Neuroblastoma patient-derived xenograft (PDX) cells were spiked into PBPC apheresis samples as a clinically relevant model system. Cells were separated by ultrasound in an acoustophoresis microchip and analyzed for recovery, purity and function using flow cytometry, quantitative real-time PCR and cell culture.

Results
PDX cells and PBPCs showed distinct size distributions, which is an important parameter for efficient acoustic separation. Acoustic cell separation did not affect neuroblastoma cell growth. Acoustophoresis allowed to effectively separate PDX cells from spiked PBPC products. When PBPCs were spiked with 10% neuroblastoma cells, recoveries of up to 98% were achieved for PDX cells while more than 90% of CD34+ stem and progenitor cells were retained in the graft. At clinically relevant tumor cell contamination rates (0.1 and 0.01% PDX cells in PBPCs), neuroblastoma cells were depleted by more than 2-log as indicated by RT-PCR analysis of PHOX2B, TH and DDC genes, while > 85% of CD34+ cells could be retained in the graft.

Conclusion
These results demonstrate the potential use of label-free acoustophoresis for PBPC processing and its potential to develop label-free, non-contact tumor cell enrichment and purging procedures for future clinical use.
Short-duration dynamic [18F]DCFPyL PET and CT perfusion imaging to localize dominant intraprostatic lesions in prostate cancer: validation against digital histopathology and comparison to [18F]DCFPyL PET/MR at 120 minutes
Authors: Yang Dae-Myoung , Alfano Ryan , Bauman Glenn , Thiessen Jonathan D. , Chin Joseph , Pautler Stephen , Moussa Madeleine , Gomez Jose A. , Rachinsky Irina , Gaed Mena , Chung Kevin J. , Ward Aaron , Lee Ting-Yim 
Published: October 2021

Abstract
Purpose
Localized prostate cancer (PCa) in patients is characterized by a dominant focus in the gland (dominant intraprostatic lesion, DIL). Accurate DIL identification may enable more accurate diagnosis and therapy through more precise targeting of biopsy, radiotherapy and focal ablative therapies. The goal of this study is to validate the performance of [18F]DCFPyL PET and CT perfusion (CTP) for detecting and localizing DIL against digital histopathological images.

Methods
Multi-modality image sets: in vivo T2-weighted (T2w)-MRI, 22-min dynamic [18F]DCFPyL PET/CT, CTP, and 2-h post-injection PET/MR were acquired in patients prior to radical prostatectomy. The explanted gland with implanted fiducial markers was imaged with T2w-MRI. All images were co-registered to the pathologist-annotated digital images of whole-mount mid-gland histology sections using fiducial markers and anatomical landmarks. Regions of interest encompassing DIL and non-DIL tissue were drawn on the digital histopathological images and superimposed on PET and CTP parametric maps. Logistic regression with backward elimination of parameters was used to select the most sensitive parameter set to distinguish DIL from non-DIL voxels. Leave-one-patient-out cross-validation was performed to determine diagnostic performance.

Results
[18F]DCFPyL PET and CTP parametric maps of 15 patients were analyzed. SUVLate and a model combining Ki and k4 of [18F]DCFPyL achieved the most accurate performance distinguishing DIL from non-DIL voxels. Both detection models achieved an AUC of 0.90 and an error rate of < 10%. Compared to digital histopathology, the detected DILs had a mean dice similarity coefficient of 0.8 for the Ki and k4 model and 0.7 for SUVLate.

Conclusions
We have validated using co-registered digital histopathological images that parameters from kinetic analysis of 22-min dynamic [18F]DCFPyL PET can accurately localize DILs in PCa for targeting of biopsy, radiotherapy, and focal ablative therapies. Short-duration dynamic [18F]DCFPyL PET was not inferior to SUVLate in this diagnostic task.
Clinical trial registration number: NCT04009174 (ClinicalTrials.gov).
Charting the complexity of the activated sludge microbiome through a hybrid sequencing strategy
Authors: Liu Lei , Wang Yulin , Yang Yu , Wang Depeng , Cheng Suk Hang , Zheng Chunmiao , Zhang Tong 
Published: October 2021

AbstractBackgroundLong-read sequencing has shown its tremendous potential to address genome assembly challenges, e.g., achieving the first telomere-to-telomere assembly of a gapless human chromosome. However, many issues remain unresolved when leveraging error-prone long reads to characterize high-complexity metagenomes, for instance, complete/high-quality genome reconstruction from highly complex systems.ResultsHere, we developed an iterative haplotype-resolved hierarchical clustering-based hybrid assembly (HCBHA) approach that capitalizes on a hybrid (error-prone long reads and high-accuracy short reads) sequencing strategy to reconstruct (near-) complete genomes from highly complex metagenomes. Using the HCBHA approach, we first phase short and long reads from the highly complex metagenomic dataset into different candidate bacterial haplotypes, then perform hybrid assembly of each bacterial genome individually. We reconstructed 557 metagenome-assembled genomes (MAGs) with an average N50 of 574 Kb from a deeply sequenced, highly complex activated sludge (AS) metagenome. These high-contiguity MAGs contained 14 closed genomes and 111 high-quality (HQ) MAGs including full-length rRNA operons, which accounted for 61.1% of the microbial community. Leveraging the near-complete genomes, we also profiled the metabolic potential of the AS microbiome and identified 2153 biosynthetic gene clusters (BGCs) encoded within the recovered AS MAGs.ConclusionOur results established the feasibility of an iterative haplotype-resolved HCBHA approach to reconstruct (near-) complete genomes from highly complex ecosystems, providing new insights into “complete metagenomics”. The retrieved high-contiguity MAGs illustrated that various biosynthetic gene clusters (BGCs) were harbored in the AS microbiome. The high diversity of BGCs highlights the potential to discover new natural products biosynthesized by the AS microbial community, aside from the traditional function (e.g., organic carbon and nitrogen removal) in wastewater treatment.
Externalizing your eating disorder: a qualitative interview study
Authors: Voswinkel Marthe M. , Rijkers Cleo , van Delden Johannes J. M. , van Elburg Annemarie A. 
Published: October 2021

Abstract
Background
Anorexia nervosa (AN) is a psychiatric disorder with an ego-syntonic nature, causing many patients to perceive their AN as part of their personal identity. Therefore, an important part of treatment is the externalization of the eating disorder, in order to help patients to perceive AN as an external influence. Studies on patient experiences of externalization in treatment for AN are sadly missing. The aims of this study were to investigate, first, patients’ perspectives on the relation between identity and anorexia nervosa (AN) and second, their experiences of an externalizing approach during treatment.

Method
A qualitative interview study was conducted including fourteen patients with AN in either Family Based Treatment, the Maudsley Model for Anorexia Nervosa Treatment for Adults, Specialist Supportive Clinical Management-Severe Enduring or Cognitive Behavioural Therapy-Enhanced.

Results
There are important differences in participants’ perceptions on how AN is related to identity. AN was perceived as part of identity, as alien or as a different side of the self. Patients’ experiences towards an externalizing approach were ambivalent. Externalization was considered helpful, whilst also evoking a negative response. Participants reported feeling as if all their behaviour was referred to as part of AN, which elicited feelings of not being taken seriously or being wrongfully accused of being dishonest.

Conclusions
First, there is considerable variation in the perceived relation between AN and identity. Second, an externalizing approach within treatment may lead to so called epistemic injustice. Awareness of these two facts is of importance for clinicians and the therapeutic relationship because that will help them to refrain from actions that can be perceived as epistemic injustice.
Recurrence eigenvalues of movements from brain signals
Authors: Pham Tuan D. 
Published: October 2021

AbstractThe ability to characterize muscle activities or skilled movements controlled by signals from neurons in the motor cortex of the brain has many useful implications, ranging from biomedical perspectives to brain–computer interfaces. This paper presents the method of recurrence eigenvalues for differentiating moving patterns in non-mammalian and human models. The non-mammalian models of Caenorhabditis elegans have been studied for gaining insights into behavioral genetics and discovery of human disease genes. Systematic probing of the movement of these worms is known to be useful for these purposes. Study of dynamics of normal and mutant worms is important in behavioral genetic and neuroscience. However, methods for quantifying complexity of worm movement using time series are still not well explored. Neurodegenerative diseases adversely affect gait and mobility. There is a need to accurately quantify gait dynamics of these diseases and differentiate them from the healthy control to better understand their pathophysiology that may lead to more effective therapeutic interventions. This paper attempts to explore the potential application of the method for determining the largest eigenvalues of convolutional fuzzy recurrence plots of time series for measuring the complexity of moving patterns of Caenorhabditis elegans and neurodegenerative disease subjects. Results obtained from analyses demonstrate that the largest recurrence eigenvalues can differentiate phenotypes of behavioral dynamics between wild type and mutant strains of Caenorhabditis elegans; and walking patterns among healthy control subjects and patients with Parkinson’s disease, Huntington’s disease, or amyotrophic lateral sclerosis.
Deformation superposition effect of asphalt mixture based on experiments and micromechanical modeling
Authors: Li Chun , Bian Xinxing , Dong Qifeng , Xu Huining 
Published: October 2021

Abstract
Under multi-wheel heavy load, the asphalt mixture is prone to exhibit the deformation superposition effect, which exacerbates the damage of pavement structure. Multi-point penetration tests and numerical simulations by discrete element method (DEM) are performed to investigate the deformation superposition effect and micromechanical characteristics of asphalt mixture. The effect of wheel spacing, wheel group, and the evolution of micromechanical deformation superposition behavior are analyzed. Results indicate that the deformation superposition resistance of the asphalt mixture under the multi-wheel load decreases dramatically with the decrease in wheel spacing and the increase in the number of wheels, specifically the wheel spacing is 54 mm and the number of wheels is 4. The DEM simulations reflect the micromechanical property of asphalt mixture in the multi-point penetration test. The reduction of tensile chains is the internal reason for asphalt mixture deformation superposition, indicating the decrease of the adhesive strength of the material. A remarkably positive correlation is found between the reduction of the tensile chain and the deformation effect coefficient. In the process of superposition, the aggregate skeleton force chains are gradually destroyed and decrease to zero until cracking. The numerical simulation outcome is consistent with the laboratory penetration test outcome.
Capitalismo, neoliberalismo progressista e lutas emancipatórias no pensamento de Nancy Fraser
Authors: Gammardella Rizzi Ester , Tranjan Tiago 
Published: October 2021

Para entender o atual cenário de crise internacional do ideário neoliberal – dos quais o Brexit, bem como a eleição de Trump e de Bolsonaro, seriam sintomas –, Nancy Fraser propõe uma ampla análise da estrutura do capitalismo, a qual engloba, mas ultrapassa, a caracterização marxista usual. De sua análise surge uma importante chave de leitura histórica: a hegemonia das últimas décadas não seria apenas do neoliberalismo, mas do que ela chama de “neoliberalismo progressista”; e a crise não é apenas do neoliberalismo, mas do progressismo que o acompanhou. Trata-se de uma análise política, que supera as análises frequentemente moralistas que têm sido feitas da ascensão de líderes populistas de direita. A reflexão acerca do caso brasileiro a partir desse instrumental pode ajudar a estabelecer, assim esperamos, cursos de ação para o futuro.
MindKind: A mixed-methods protocol for the feasibility of global digital mental health studies in young people
Authors:   The MindKind Consortium
Published: October 2021

Background: While an estimated 14-20% of young adults experience mental health conditions worldwide, the best strategies for prevention and management are not fully understood. The ubiquity of smartphone use among young people makes them excellent candidates for collecting data about lived experiences and their relationships to mental health. However, not much is known about the factors affecting young peoples’ willingness to share information about their mental health. Objective: We aim to understand the data governance and engagement strategies influencing young peoples’ (aged 16-24) participation in app-based studies of mental health. We hypothesize that the willingness to participate in research is impacted by their ability to be involved in how their data is collected, shared, and used. Methods: Here, we describe the MindKind Study, which employs mixed methods to understand the feasibility of global, smartphone-based studies of youth mental health. A pilot 12-week app-based substudy will query participants’ willingness to engage with remote mental health studies. Participants will be randomized into one of four different data governance models designed to understand their preferences, as well as the acceptability of models that allow them more or less control over how their data are accessed and used. Enrolees will receive one of two different engagement strategies. A companion qualitative study will employ a deliberative democracy approach to examine the preferences, concerns and expectations of young people, with respect to remote mental health research. We also detail our engagement with young people as co-researchers in this study. This pilot study is being conducted in India, South Africa and the United Kingdom. Conclusions: This study is expected to generate new insights into the feasibility of, and best practices for, remote smartphone-based studies of mental health in youth and represents an important step toward understanding which approaches could help people better manage their mental health.
Stretchable optical diffraction grating from poly(acrylic acid)/polyethylene oxide stereocomplex
Authors: He Jinghan , Kovach Andre , Wang Yunxiang , Wang William , Wu Wei , Armani Andrea M. 
Published: October 2021

Advances in optical materials, which were initially static elements, have enabled dynamically tunable optical diffraction gratings to be designed. One common tuning strategy relies on mechanical deformation of the grating pitch to modify the diffraction pattern. In the present work, we demonstrate an all-polymer tunable diffraction grating fabricated using a modified replica molding process. The poly(acrylic acid) (PAA)/polyethylene oxide (PEO) polymer stereocomplex films exhibit optical transmittance at or above 80% from 500 nm to 1400 nm and stretchability over 800% strain with reversibility under 70% strain. The imprinted gratings are characterized at 633 nm and 1064 nm under a range of strain conditions. The measured tunability agrees with finite element method modeling.