bluuebunny/crossref_metadata_2025_split huggingface dataset:
Human Olfactory Ensheathing Cell-derived Extracellular Cesicles: miRNA Profile and Neuroprotective Effect
Authors: Tu Yuan-Kun , Hsueh Yu-Huan , Huang Hsien-Chang 
Published: October 2021

Background:
Extracellular Vesicle (EV)-based therapy has been identified as a leading
alternative approach in several disease models. EV derived from the Olfactory Ensheathing Cell
(OEC) has been documented for its strong neuro-regenerative capacity. However, no information
on its cargo that may contribute to its therapeutic effect has been available.

Objective:
To report the first miRNA profile of human OEC (hOEC) -EV, and investigate the neuroprotective
effects.

Methods:
hOEC-EV was isolated and sequenced. We established in vitro experiments to assess the
therapeutic potential of hOEC-EVs with respect to insulted neural progenitor cells (NPCs), and the
angiogenesis effect. Secondary post-injury insults were imitated using t-BHP-mediated oxidative
stress.

Results:
We noted a strong abundance of hOEC-EV-miRNAs, including hsa-miR148a-3p, hasmiR151a-
3p and several members of let-7 family. The common targets of 15 miRNAs among the
top 20 miRNAs were thrombospondin 1 and cyclin dependent kinase 6. We demonstrated that
hOEC-EVs promote normal NPC proliferation and differentiation to neuron-like morphologies
with prolonged axons. hOEC-EVs protect cells from t-BHP mediated apoptosis. We also found
that the migration rate of either NPCs or endothelial cells significantly improved with hOEC-EVs.
Furthermore, in vitro tube formation assays indicated that angiogenesis, an important process for
tissue repair, was significantly enhanced in human umbilical vein endothelial cells exposed to
hOEC-EVs.

Conclusion:
Our results revealed that hOEC-EVs exert neuroprotective effects by protecting cells
from apoptosis and promoting in vitro biological processes that are important to neural tissue repair,
including neural cell proliferation, axonal growth, and cell migration, in addition to enhancing
angiogenesis.
A Novel Design of Multi-epitope Peptide Vaccine Against Pseudomonas
aeruginosa
Authors: Esmaeilzadeh Fatemeh , Mahmoodi Shirin 
Published: October 2021

Background:
As an opportunistic pathogen, Pseudomonas aeruginosa causes many different
hazardous infections. The high mortality rate resulting from infection with this antibiotic-resistant pathogen
has made it a major challenge in clinical treatment; it has been listed as the most harmful bacterium to
humans by the WHO. So far, no vaccine has been approved for P. aeruginosa.

Objective:
Infections performed by bacterial attachment and colonization with type IV pili (T4P), known
as the most essential adhesive vital for adhesion, while pilQ is necessary for the biogenesis of T4P, also
outer membrane proteins of a pathogen is also effective in stimulating the immune system; in this regard,
pilQ, OprF, and OprI, are excellent candidate antigens for production of an effective vaccine against P.
aeruginosa.

Methods:
In this research, various bioinformatics methods were employed in order to design a new multiepitope
peptide vaccine versus P. aeruginosa. Since T CD4+ cell immunity is important in eradicating P.
aeruginosa, OprF, OprI, and pilQ antigens were analyzed to determine Helper T cell Lymphocyte (HTL)
epitopes by many different immunoinformatics servers. One of the receptor agonists 2 (TLR2), a segment
of the Por B protein from Neisseria meningitides was used as an adjuvant in order to stimulate an effective
cellular immune response, and suitable linkers were used to connect all the above mentioned parts. In
the vaccine construct, linear B cell epitopes were also identified.

Results:
Conforming the bioinformatics forecasts, the designed vaccine possesses high antigenicity and is
not allergen.

Conclusion:
In this regard, the designed vaccine candidate is strongly believed to possess the potential of
inducing cellular and humoral immunity against P. aeruginosa.
Packet Scheduling for Internet of Remote Things (IoRT) Devices in Next
Generation Satellite Networks
Authors: Aiyetoro Gbolahan , Owolawi Pius 
Published: October 2021

Background:
The massive amount of deployment of Internet of Things (IoT) devices via
wireless communications has presented a new paradigm in next-generation mobile networks. The rapid
growth in the deployment of IoT devices can be linked to the diverse use of several IoT applications
for home automation, smart systems, and other forms of innovations in businesses and industry
4.0.

Methods:
There is a need for a robust network infrastructure to actualize the huge traffic demand of
IoT communications in this new paradigm across the globe, including rural and remote areas. However,
due to technical and economic constraints, the terrestrial network infrastructure is not able to fulfill
this requirement. Hence, the need for satellite network infrastructure. This solution will be of immense
benefit to the provision of remote health care, disaster management, remote sensing, and asset tracking,
and environmental monitoring, to name a few. While this remains an interesting solution, packet
scheduling, which is one of the key radio resource management functions, is still a challenging issue
that remains undefined, especially in a satellite network scenario that has its peculiarities and challenges.

Results:
Hence, the goal of this research work is to design a new packet scheduling scheme suitable
for machine-type communications and also mixed-use case scenarios in satellite network scenarios.
The performance evaluation of the proposed packet scheduler is conducted through simulations.

Conclusion:
The newly proposed packet scheduling scheme provides an improvement of approximately
7 Mbps and 0.5 bps/Hz in terms of throughput and spectral efficiency performances, respectively,
in mixed-use case scenarios, when compared to known throughput optimal packet schedulers,
without serious compromise to other performance metrics.
Exploring the Effects of In-App Components on Engagement With a Symptom-Tracking Platform Among Participants With Major Depressive Disorder (RADAR-Engage): Protocol for a 2-Armed Randomized Controlled Trial
Authors: White Katie M , Matcham Faith , Leightley Daniel , Carr Ewan , Conde Pauline , Dawe-Lane Erin , Ranjan Yatharth , Simblett Sara , Henderson Claire , Hotopf Matthew 
Published: October 2021

Background
Multi-parametric remote measurement technologies (RMTs) comprise smartphone apps and wearable devices for both active and passive symptom tracking. They hold potential for understanding current depression status and predicting future depression status. However, the promise of using RMTs for relapse prediction is heavily dependent on user engagement, which is defined as both a behavioral and experiential construct. A better understanding of how to promote engagement in RMT research through various in-app components will aid in providing scalable solutions for future remote research, higher quality results, and applications for implementation in clinical practice.

Objective
The aim of this study is to provide the rationale and protocol for a 2-armed randomized controlled trial to investigate the effect of insightful notifications, progress visualization, and researcher contact details on behavioral and experiential engagement with a multi-parametric mobile health data collection platform, Remote Assessment of Disease and Relapse (RADAR)–base.

Methods
We aim to recruit 140 participants upon completion of their participation in the RADAR Major Depressive Disorder study in the London site. Data will be collected using 3 weekly tasks through an active smartphone app, a passive (background) data collection app, and a Fitbit device. Participants will be randomly allocated at a 1:1 ratio to receive either an adapted version of the active app that incorporates insightful notifications, progress visualization, and access to researcher contact details or the active app as usual. Statistical tests will be used to assess the hypotheses that participants using the adapted app will complete a higher percentage of weekly tasks (behavioral engagement: primary outcome) and score higher on self-awareness measures (experiential engagement).

Results
Recruitment commenced in April 2021. Data collection was completed in September 2021. The results of this study will be communicated via publication in 2022.

Conclusions
This study aims to understand how best to promote engagement with RMTs in depression research. The findings will help determine the most effective techniques for implementation in both future rounds of the RADAR Major Depressive Disorder study and, in the long term, clinical practice.

Trial Registration
ClinicalTrials.gov NCT04972474; http://clinicaltrials.gov/ct2/show/NCT04972474

International Registered Report Identifier (IRRID)
DERR1-10.2196/32653
Apps e-health en los procesos de enseñanza aprendizaje en enfermería
Authors: Cortes Restrepo Nina Vanesa 
Published: October 2021

En la actualidad las aplicaciones móviles descargadas en los celulares se están convirtiendo en instrumentos útiles para los procesos de aprendizaje. Con el auge de la tecnología móvil y la conexión permanente a la red de Internet, se genera apertura a una serie de alternativas en el área de la salud y en la educación de los profesionales de esta área. Las aplicaciones móviles surgen brindando múltiples oportunidades de dinamizar los procesos cognitivos. Se encuentran varias de estas encaminadas a enseñanza – aprendizaje en aprendices de enfermería. El presente artículo tiene por objeto representar el posible uso de las APPS E-Health en métodos de enseñanza aprendizaje en enfermería. Método: La metodología que se empleo fue un estudio de tipo descriptivo transversal. La muestra definitiva fue de 35 artículos que cumplieron con los criterios de búsqueda de acuerdo con las categorías creadas. Resultados: El uso de aplicaciones móviles en educación es cada vez más usual. No obstante, no se encuentran estudios que permitan avalar los procesos de calidad en los contenidos implícitos y las posibles repercusiones que puede generar en el aprendizaje. Se analiza su impacto dado el momento de evolución tecnológica, se plantea el proyecto de Mobile Learning 2020 donde se pretende desarrollar la metodología educativa basada en aplicaciones mHealth que servirán como herramientas pedagógicas en la formación de cuidado de enfermería.
Barreras y oportunidades para el acceso a los servicios de salud según la experiencia de cuidadores de niños y adolescentes con cáncer
Authors: Ávila Mora Juan Carlos , Rueda Lizarazo Monica Paola , Rodríguez Delgado Katherine Vanessa , Olaya Jurado Jessica , Bustamante Luis Arturo , Mesa Pérez Rafael 
Published: October 2021

El cáncer infantil es una patología con múltiples desencadenantes primarios y está relacionado con los determinantes de la salud, en el que el acceso a los servicios y el aseguramiento para la protección del riesgo financiero son aspectos relevantes para el diagnóstico temprano, manejo y tratamiento de la enfermedad. Se planteó una investigación para analizar las barreras y oportunidades para el acceso a los servicios de salud según la experiencia de cuidadores que apoyan el control del cáncer en niños y adolescentes en Colombia, por medio de la realización de un estudio de enfoque cualitativo y diseño hermenéutico interpretativo por medio de la realización de nueve grupos focales en 36 cuidadoras de niños diagnosticados con cáncer en distintas fundaciones en cuatro ciudades de Colombia (Cali, Cartagena, Medellín y Santa Marta). Los datos fueron analizados en el programa Nvivo y se codificó la información obtenida para crear en total cinco categorías de análisis y sus respectivas subcategorías: 1. Disponibilidad de los servicios, 2. Accesibilidad física, organizacional y financiera, 3. Aceptabilidad de los servicios, 4. Contacto con el servicio y 5. Oportunidades de acceso. Se encontró que las principales barreras se relacionan con el acceso a los servicios de salud y los trámites administrativos relacionados al mismo, dificultades para la continuación del tratamiento por carencia de los medios para efectuar el mismo, lo que conlleva a la implementación de recursos jurídicos. Se evidencia un cambio en los estilos de vida de las cuidadoras principales que también impacta al núcleo familiar en sus múltiples dimensiones ya que la mayoría de los miembros de este tienen que modificar sus rutinas. Se plantea que los pacientes con este tipo de patologías se encuentren en una ruta diferencial de atención en el sistema salud permitiendo la realización de diagnósticos tempranos y una adecuada continuidad del tratamiento.
Relationships between congenital peritoneopericardial diaphragmatic hernia or congenital central diaphragmatic hernia and ductal plate malformations in dogs and cats
Authors: Seibert Laura M. , Center Sharon A. , Randolph John F. , Miller Meredith L. , Miller Andrew D. , Choi Eunju , Flanders James A. , Harvey H. Jay 
Published: October 2021

Abstract

OBJECTIVE
To characterize the association between peritoneopericardial diaphragmatic hernia (PPDH) or congenital central diaphragmatic hernia (CCDH) and ductal plate malformations (DPMs) in dogs and cats.

ANIMALS
18 dogs and 18 cats with PPDH or CCDH and 19 dogs and 18 cats without PPDH or CCDH.

PROCEDURES
Evaluation of clinical details verified PPDH or CCDH and survival times. Histologic features of nonherniated liver samples were used to categorize DPM. Immunohistochemical staining for cytokeratin-19 distinguished bile duct profiles per portal tract and for Ki-67–assessed cholangiocyte proliferation. Histologic features of herniated liver samples from PPDH or CCDH were compared with those of pathological controls (traumatic diaphragmatic hernia, n = 6; liver lobe torsion, 6; ischemic hepatopathy, 2).

RESULTS
DPM occurred in 13 of 18 dogs with the proliferative-like phenotype predominating and in 15 of 18 cats with evenly distributed proliferative-like and Caroli phenotypes. Congenital hepatic fibrosis DPM was noted in 3 dogs and 2 cats and renal DPM in 3 dogs and 3 cats. No signalment, clinical signs, or clinicopathologic features discriminated DPM. Kaplan Meier survival curves were similar in dogs and cats. Bile duct profiles per portal tract in dogs (median, 5.0; range, 1.4 to 100.8) and cats (6.6; 1.9 to 11.0) with congenital diaphragmatic hernias significantly exceeded those in healthy dogs (1.4; 1.2 to 1.6) and cats (2.3; 1.7 to 2.6). Animals with DPM lacked active cholangiocyte proliferation. Histologic features characterizing malformative bile duct profiles yet without biliary proliferation were preserved in herniated liver lobes in animals with DPM.

CONCLUSIONS AND CLINICAL RELEVANCE
DPM was strongly associated with PPDH and CCDH. Because DPM can impact health, awareness of its coexistence with PPDH or CCDH should prompt biopsy of nonherniated liver tissue during surgical correction of PPDH and CCDH.
Transphyseal screw placement with and without hemicircumferential periosteal transection and elevation for correction of severe bilateral carpal varus deformities in an alpaca cria
Authors: McQuillan Siobhan , Vermedal Hanna , O’Leary John Mark , Chandler Chyanne , Kearney Clodagh 
Published: October 2021

Abstract

CASE DESCRIPTION
A 2.5-month-old 17.5-kg female alpaca cria was presented for evaluation and treatment of severe bilateral carpal varus deformities.

CLINICAL FINDINGS
No lameness was evident at a walk, and neither carpal varus deformity could be corrected by means of manipulation. Radiography revealed severe varus of the left (27°) and right (21°) carpal regions. No additional conformational abnormalities were detected.

TREATMENT AND OUTCOME
A single 2.7-mm transphyseal cortical screw was placed in the distolateral aspect of the radius in each limb. On reexamination 8 weeks after screw placement, the left carpal varus deformity had corrected from 27° to 2.6°, and the left transphyseal screw was removed. The right carpal varus deformity had improved but was still present (18°), and hemicircumferential periosteal transection and elevation was performed on the mediodistal aspect of the right radius. Five weeks after the second surgery, the right carpal varus deformity had corrected to 2.4°, and the right transphyseal screw was removed. Six months after the second screw removal, both thoracic limbs remained straight, the cria had a normal gait, and the owner was happy with the cosmetic result.

CLINICAL RELEVANCE
Placement of a single transphyseal cortical screw with or without the addition of hemicircumferential periosteal transection and elevation can provide a favorable outcome in skeletally immature alpacas with severe carpal varus deformities.
Honeybees as a teaching tool in veterinary education
Authors: Mayer Joerg 
Published: October 2021

Abstract

Abstract
Veterinary education is becoming more and more complex, but the ultimate goal—providing an education that will prepare students for entry-level positions in the profession—remains the same. Often, hands-on work with living animals aimed at achieving core competencies is relegated to the final year of the veterinary curriculum. But, incorporating honeybees allows introduction of these important concepts during earlier years of veterinary student training. In addition, honeybees are under severe threat from a multitude of health problems, and this has dire implications for our own food supply. Veterinarians need to be actively involved in addressing this health crisis. Ever since the US FDA implemented its Veterinary Feed Directive rule, which dictates how certain antimicrobials can legally be administered in the feed or water of food-producing animals, and made changes to its policy on the use of medically necessary antimicrobials on bees, honeybees have fallen under the direct purview of veterinarians, highlighting the need for greater literacy in honeybee health. The present manuscript describes reasons why and ways how honeybees can play a larger role in the education of veterinarians in the United States.
Integration of Natural and Artificial Light on Energy Efficiency of the Mega Bank Makassar Tower Building
Authors: Ismail Isty Cahyani , Rahim Ramli , Hamzah Baharuddin 
Published: October 2021

One of the largest energy consumers in the world is buildings. The energy consumption comes from the lighting system. Energy use in
buildings is generally 25% for lighting systems. The strategy used in building design is to reduce energy consumption while maintaining the
best comfort in a building. The application of energy-saving concepts from the building sector is optimizing the lighting system by integrating
natural and artificial lighting systems. This study aims to determine the light intensity in the integrated lighting system of natural and artificial
manually and also to find out how much energy can be saved with the integrated lighting system manually. The research location is at the
Mega Bank Makassar Tower Building. The research sample was selected by purposive sampling and the sixth floor was chosen as the
research location. In this study, simulations were carried out using the DIAlux 4.13 program to integrate natural and artificial light and to
calculate the amount of energy efficiency in the workspace. To obtain optimal light intensity and energy savings, a simulation was carried
out by turning off half the light points in the workspace, especially the light points around the building openings. The simulation results show
that the average integrated lighting quality meets the minimum lighting requirements and can save energy usage by up to 50%.
Keywords: energy efficiency; integration lighting; workspace
Analisis Vegetasi di Kawasan Petilasan Mbah Maridjan Taman Nasonal Gunung Merapi
Authors: Shabirin Amri , Puteri Yuliantika , Syafira Hendrianis , Mayasari Tia , Nurkhasanah Mufti 
Published: October 2021

This research aims to determine the diversity of habitus and species that dominate in the region of Kinahrejo, Umbulharjo, Cangkringan, Sleman. The method used is square sampling by making a 7x7 meters square plot of 2 plots so that an area of ​​98 m2 is obtained. Vegetation analysis using the method of observation, and monitoring (field observation) directly with random sampling and using the principle of representative. Analysis the level of habitat diversity using the Shannon-wienner Index and determination of species that dominate using the calculation of the Important Value Index (INP). A total of 34 species consisting of mosses, shrubs, herbs, and stand-structure are occupy the observation area. The results of the research and analysis showed that Tamarindus indica, which included stand-structure habitus had the highest INP of 139.54% and the lowest INP was found in shrubs habitus. The highest Shannon-wienner index in shrubs habitus is 1.220928 and the lowest is in stand-structure habitus. It can be concluded, stand-structure habitus dominates the area while, the highest diversity is found in shurbs habitus. The eruption in 2010 caused damage to ecosystems and invasive plants to become dominant such as Acacia decurrens and shrubs.
Formal Argument and Olympiodorus’ Development as a Plato-Commentator
Authors: Tarrant Harold 
Published: October 2021

Abstract
Olympiodorus led the Platonist school of philosophy at Alexandria for several decades in the sixth century, and both Platonic and Aristotelian commentaries ascribed to him survive. During this time the school’s attitude to the teaching of Aristotelian syllogistic, originally owing something to Ammonius, changed markedly, with an early tendency to reinforce the teaching of syllogistic even in Platonist lectures giving way to a greater awareness of its limitations. The vocabulary for arguments and their construction becomes far commoner than the language of syllogistic and syllogistic figures, and also of demonstration. I discuss the value of these changes for the dating of certain works, especially where the text lectured on does not demand different emphases. The commitment to argument rather than to authority continues, but a greater emphasis eventually falls on the establishment of the premises than on formal validity.
Logic and Interpretation: Syllogistic Reconstructions in Simplicius’ Commentary on Aristotle’s Physics
Authors: Harari Orna 
Published: October 2021

Abstract
In this article I explain three puzzling features of Simplicius’ use of syllogistic reconstructions in his commentary on Aristotle’s Physics: (1) Why does he reconstruct Aristotle’s non-argumentative remarks? (2) Why does he identify the syllogistic figure of an argument but does not explicitly present its reconstruction? (3) Why in certain lemmata does he present several reconstructions of the same argument? Addressing these questions, I argue that these puzzling features are an expression of Simplicius’ assumption that formal reasoning underlies Aristotle’s prose, hence they reflect his attempt to capture as faithfully as possible Aristotle’s actual mode of reasoning. I show further that, as a consequence of this seemingly descriptive use of syllogistic reconstructions, logic serves Simplicius not only as an expository and clarificatory tool of certain interpretations or philosophical views, but also motivates and shapes his exegetical stances and approach.