bluuebunny/crossref_metadata_2025_split huggingface dataset:
High frequency of enterotoxin encoding genes of Staphylococcus aureus isolated from food and clinical samples
Authors: Haghi Fakhri , Zeighami Habib , Hajiloo Zeynab , Torabi Neda , Derakhshan Safoura 
Published: June 2021

Abstract
Background
Staphylococcus aureus is recognized as an important cause of food poisoning related to the consumption of raw, undercooked, or mishandled foods worldwide.

Methods
A total of 90 individual meat samples and 200 clinical specimens were collected and investigated the frequency of S. aureus and classical enterotoxin genes. The samples were cultured on Baird-Parker and Mannitol salt agar and subjected for confirmatory biochemical tests and molecular detection of femA, sea, seb, sec, sed, and see genes.

Results
A total of 31 (34.5%) meat samples and 81 (40.5%) clinical specimens were positive for the presence of S. aureus. These isolates were detected with slightly higher frequency in clinical specimens than food samples (P> 0.05). Furthermore, the frequency of S. aureus in raw meat (23.4%) was higher than that in cooked meat samples (11.1%) (P< 0.05). Staphylococcal enterotoxin (SE) genes were identified in 18 (58.1%) of 31 meat isolates and 42 (51.8%) of 81 clinical isolates. The frequency of SE genes (except see) in meat isolates was slightly higher than that in clinical isolates (P> 0.05). We found sea and see genes with higher frequency than others in both meat and clinical samples. Furthermore, 55.5% of meat isolates and 38.1% of clinical isolates possessed more than one se gene.

Conclusion
Detection of enterotoxigenic S. aureus in clinical and raw meat samples shows a probable risk for public health. Therefore, intensive and continuous monitoring of potentially pathogenic S. aureus is strongly recommended in order to evaluate the human health risk arising from food consumption.
G6PD deficiency among malaria-infected national groups at the western part of Myanmar with implications for primaquine use in malaria elimination
Authors: Han Kay Thwe , Han Zay Yar , Aye Kyin Hla , Wai Khin Thet , Thi Aung , Cui Liwang , Sattabongkot Jetsumon 
Published: June 2021

Abstract
Background
Glucose 6-phosphate dehydrogenase deficiency (G6PDd) plays a central role in readiness assessment for malaria elimination in Myanmar by 2030 that includes primaquine (PQ) use. The risk of hemolysis in G6PDd individuals hampers the widespread use of primaquine safely in malaria-infected patients. In the pre-elimination era, it is important to screen initially for asymptomatic malaria in combination with G6PD deficiency by applying more sensitive diagnostic tools. Therefore, this study examined the proportion of G6PDd and the distribution of G6PD genotypes among malaria-infected national groups in Myanmar before initiation of malaria elimination strategies.

Methods
A cross-sectional study in one township each with high malaria burden from two states in the western part of Myanmar, was conducted during 2016-2018, and 320 participants (164 Rakhine and 156 Chin National groups) were recruited. We used RDT and ultrasensitive polymerase chain reaction (us PCR) method to confirm malaria infection, and a G6PD RDT(CareStart) to detect G6PDd and PCR/restriction fragment length polymorphism (RFLP) method to confirm the variant of G6PDd for genotyping. G6PD enzyme activity was measured by G6PD Biosensor (CareStart).

Results
Malaria positivity rates detected by RDT were lower than those detected by us PCR in the combined samples [13% (42/320) vs. 21% (67/320)] as well as in the Rakhine samples [17% (28/164) vs. 25% (41/164)] and in Chin samples [9% (14/156) vs. 17% (26/156)]. G6PD deficiency rates were approximately 10% in both the combined samples and specific national groups. For G6PD enzyme activity in the combined samples, G6PDd (defined as < 30% of adjusted male median) was 10% (31/320) and severe G6PDd (< 10% of AMM) was 3% (9/320). Among malaria-infected patients with positive by both RDT and usPCR, G6PDd was less than 20% in each national group. G6PD genotyping showed that the G6PD Mahidol (G487A) was the major variant.

Conclusions
The varying degree of G6PDd detected among malaria-infected national groups by advanced diagnostic tools, strongly support the recommend G6PD testing by the National Malaria Control Program and the subsequent safe treatment of P. vivax by primaquine for radical cure. Establishing a field monitoring system to achieve timely malaria elimination is mandatory to observe the safety of patients after PQ treatment.
Biologic Assignment Trial of Reduced-Intensity Hematopoietic Cell Transplantation Based on Donor Availability in Patients 50-75 Years of Age With Advanced Myelodysplastic Syndrome
Authors: Nakamura Ryotaro , Saber Wael , Martens Michael J. , Ramirez Alyssa , Scott Bart , Oran Betul , Leifer Eric , Tamari Roni , Mishra Asmita , Maziarz Richard T. , McGuirk Joseph , Westervelt Peter , Vasu Sumithira , Patnaik Mrinal , Kamble Rammurti , Forman Stephen J. , Sekeres Mikkael A. , Appelbaum Frederick , Mendizabal Adam , Logan Brent , Horowitz Mary , Cutler Corey 
Published: June 2021

PURPOSE Allogeneic hematopoietic cell transplantation (HCT) is the only potentially curative therapy for myelodysplastic syndromes (MDS), although it is infrequently offered to older patients. The relative benefits of HCT over non-HCT therapy in older patients with higher-risk MDS have not been defined. METHODS We conducted a multicenter biologic assignment trial comparing reduced-intensity HCT to hypomethylating therapy or best supportive care in subjects 50-75 years of age with intermediate-2 or high-risk de novo MDS. The primary outcome was overall survival probability at 3 years. Between January 2014 and November 2018, we enrolled 384 subjects at 34 centers. Subjects were assigned to the Donor or No-Donor arms according to the availability of a matched donor within 90 days of study registration. RESULTS The median follow-up time for surviving subjects was 34.2 months (range: 2.3-38 months) in the Donor arm and 26.9 months (range: 2.4-37.2 months) in the No-Donor arm. In an intention-to-treat analysis, the adjusted overall survival rate at 3 years in the Donor arm was 47.9% (95% CI, 41.3 to 54.1) compared with 26.6% (95% CI, 18.4 to 35.6) in the No-Donor arm ( P = .0001) with an absolute difference of 21.3% (95% CI, 10.2 to 31.8). Leukemia-free survival at 3 years was greater in the Donor arm (35.8%; 95% CI, 29.8 to 41.8) compared with the No-Donor arm (20.6%; 95% CI, 13.3 to 29.1; P = .003). The survival benefit was seen across all subgroups examined. CONCLUSION We observed a significant survival advantage in older subjects with higher-risk MDS who have a matched donor identified and underwent reduced-intensity HCT, when compared with those without a donor. HCT should be included as an integral part of MDS management plans in fit older adults with higher-risk MDS.
Relationship Between Intraoperative and Preoperative Ambulatory Nighttime Heart Rates: A Secondary Analysis of a Prospective Observational Study
Authors: Kouz Karim , Hoppe Phillip , Reese Philip , Burfeindt Christian , Flick Moritz , Briesenick Luisa , Nitzschke Rainer , Pinnschmidt Hans , Saugel Bernd 
Published: June 2021

BACKGROUND:
It remains unknown what constitutes physiologically relevant intraoperative bradycardia. Intraoperative bradycardia is usually defined using absolute heart rate thresholds, ignoring preoperative baseline heart rates. In contrast, we considered defining intraoperative bradycardia relative to preoperative ambulatory nighttime heart rate. Specifically, we hypothesized that the individual mean intraoperative heart rate is lower than the mean preoperative ambulatory nighttime heart rate. We, therefore, sought to investigate the relationship between the intraoperative and preoperative ambulatory nighttime heart rates in adults having noncardiac surgery with general anesthesia. Additionally, we sought to investigate the incidence of intraoperative bradycardia using relative versus absolute heart rate thresholds.

METHODS:
We conducted a secondary analysis of a database from a prospective study including preoperative ambulatory and intraoperative heart rates in 363 patients having noncardiac surgery with general anesthesia.

RESULTS:
The mean intraoperative heart rate was lower than the mean nighttime heart rate (mean difference, −9 bpm; 95% confidence interval [CI], −10 to −8 bpm; P < .001). The mean intraoperative heart rate was lower than the mean nighttime heart rate in 319 of 363 patients (88%; 95% CI, 84%–91%). The incidence of intraoperative bradycardia was 42% (95% CI, 38%–47%) when it was defined as intraoperative heart rate >30% lower than mean nighttime heart rate and 43% (95% CI, 38%–49%) when it was defined as intraoperative heart rate <45 bpm.

CONCLUSIONS:
The mean intraoperative heart rate is lower than the mean nighttime heart rate in about 9 of 10 patients. Intraoperative bradycardia might thus be physiologically and clinically important. Future research needs to investigate whether there is an association between intraoperative bradycardia and postoperative outcomes.
Neither Peristaltic Pulse Dynamic Compressions nor Heat Therapy Accelerate Glycogen Resynthesis after Intermittent Running
Authors: KIM KYOUNGRAE , KARGL CHRISTOPHER K. , RO BOHYUN , SONG QIFAN , STEIN KIMBERLY , GAVIN TIMOTHY P. , ROSEGUINI BRUNO T. 
Published: June 2021

ABSTRACT

Purpose
To investigate the effects of a single session of either peristaltic pulse dynamic leg compressions (PPDC) or local heat therapy (HT) after prolonged intermittent shuttle running on skeletal muscle glycogen content, muscle function, and the expression of factors involved in skeletal muscle remodeling.

Methods
Twenty-six trained individuals were randomly allocated to either a PPDC (n = 13) or a HT (n = 13) group. After completing a 90-min session of intermittent shuttle running, participants consumed 0.3 g·kg−1 protein plus 1.0 g·kg−1 carbohydrate and received either PPDC or HT for 60 min in one randomly selected leg, while the opposite leg served as control. Muscle biopsies from both legs were obtained before and after exposure to the treatments. Muscle function and soreness were also evaluated before, immediately after, and 24 h after the exercise bout.

Results
The changes in glycogen content were similar (P > 0.05) between the thigh exposed to PPDC and the control thigh ~90 min (Control: 14.9 ± 34.3 vs PPDC: 29.6 ± 34 mmol·kg−1 wet wt) and ~210 min (Control: 45.8 ± 40.7 vs PPDC: 52 ± 25.3 mmol·kg−1 wet wt) after the treatment. There were also no differences in the change in glycogen content between thighs ~90 min (Control: 35.9 ± 26.1 vs HT: 38.7 ± 21.3 mmol·kg−1 wet wt) and ~210 min (Control: 61.4 ± 50.6 vs HT: 63.4 ± 17.5 mmol·kg−1 wet wt) after local HT. The changes in peak torque and fatigue resistance of the knee extensors, muscle soreness, and the mRNA expression and protein abundance of select factors were also similar (P > 0.05) in both thighs, irrespective of the treatment.

Conclusions
A single 1-h session of either PPDC or local HT does not accelerate glycogen resynthesis and the recovery of muscle function after prolonged intermittent shuttle running.
Sex Differences in Neuromuscular Fatigue and Changes in Cost of Running after Mountain Trail Races of Various Distances
Authors: BESSON THIBAULT , PARENT AUDREY , BROWNSTEIN CALLUM G. , ESPEIT LOÏC , LAPOLE THOMAS , MARTIN VINCENT , ROYER NICOLAS , RIMAUD DIANA , SABATER PASTOR FREDERIC , SINGH BENJAMIN , VARESCO GIORGIO , ROSSI JEREMY , TEMESI JOHN , MILLET GUILLAUME Y. 
Published: June 2021

ABSTRACT

Introduction
Women have been shown to experience less neuromuscular fatigue than men in knee extensors (KE) and less peripheral fatigue in plantar flexors (PF) after ultratrail running, but it is unknown if these differences exist for shorter trail running races and whether this may impact running economy. The purpose of this study was to characterize sex differences in fatigability over a range of running distances and to examine possible differences in the postrace alteration of the cost of running (Cr).

Methods
Eighteen pairs of men and women were matched by performance after completing different races ranging from 40 to 171 km, divided into SHORT versus LONG races (<60 and >100 km, respectively). Neuromuscular function and Cr were tested before and after each race. Neuromuscular function was evaluated on both KE and PF with voluntary and evoked contractions using electrical nerve (KE and PF) and transcranial magnetic (KE) stimulation. Oxygen uptake, respiratory exchange ratio, and ventilation were measured on a treadmill and used to calculate Cr.

Results
Compared with men, women displayed a smaller decrease in maximal strength in KE (−36% vs −27%, respectively, P < 0.01), independent of race distance. In SHORT only, women displayed less peripheral fatigue in PF compared with men (Δ peak twitch: −10% vs −24%, respectively, P < 0.05). Cr increased similarly in men and women.

Conclusions
Women experience less neuromuscular fatigue than men after both “classic” and “extreme” prolonged running exercises but this does not impact the degradation of the energy Cr.
The Yellowstone magmatic-hydrothermal system: using radiogenic and stable isotope geochemistry to constrain the processes of water-rock interaction
Authors: Messa Cole 
Published: June 2021

The hot springs of Yellowstone National Park provide a broad range of isotopic data (e.g. 238U-, 235U-, and 232Th-series) that can be exploited to interpret the geochemical processes occurring at depth, including water-rock interaction, nuclide sourcing, and fluid residence times. Despite its worldwide notoriety, Yellowstone’s hydrothermal system remains largely unconstrained. While major advances in the past century have helped us to understand the highly varied geochemical characteristics of Yellowstone’s thermal features and their potential mechanisms of formation, many questions remain regarding where exactly the water resides before ascending to the surface, how long the water remains at depth, and what geochemical processes are occurring between these waters and the superheated aquifer rocks. One of the primary questions surrounding the Yellowstone hydrothermal system revolves around the concept of “phase separation”, whereby ascending, pressurized hydrothermal fluids undergo decompressional boiling and separate into an acidic vapor phase and a neutral fluid phase. These diverging phases result in the two dominant spring chemistries viewed on the surface, acid-sulfate springs and neutral-chloride springs. Still, little is known about the timescales such a process operates on, and what geochemical parameters can be constrained to support the existence of this model. Herein we examine a handful of hydrothermal features throughout Yellowstone National Park in an effort to investigate the likelihood of phase separation’s existence and whether or not the isotopic evidence supports the geochemical processes that we know to be occurring should this model persist within the plumbing of a continental hydrothermal system.

Featured photo from figure 3 in report.
Factors influencing perceived appropriateness of concessioner activity in Grand Teton
Authors: Pitas Nicholas 
Published: June 2021

Concessioner provided services are integral to the national park visitor experience, and with visitation across NPS units growing steadily, services provided by these public-private-partnerships will likely only increase in importance. Despite this, concerns exist regarding the presence of for-profit entities within national parks. While private businesses may be more responsive to consumers, their presence raises questions regarding equity, access, and perceptions of ownership. The purpose of this study was to assess factors that may influence visitor’s perceptions of appropriateness regarding (a) current and (b) future concessioner activities within Grand Teton National park (GTNP). Regression analyses indicate the importance of personal values, rather than actual experiences (positive or negative) with concessions in shaping perceived appropriateness of future concessions activity. Satisfaction with concessions services, amount of concessions activity that respondents perceived in the park in the present, and trust in GTNP were all non-factors in determining anticipated future appropriateness. Instead, a belief that concessions activity would increase, social liberalism, economic conservatism, and place identity with GTNP were related to perceptions that concessioner activity at GTNP would be inappropriately high in the future. Although the regression predicting future beliefs was robust (R2 = 0.43), no variables significantly predicted current beliefs (R2 = 0.05).

Featured photo by Grand Teton on Flickr. https://flic.kr/p/2jf3fa9
Semiconductor-based selective emitter with a sharp cutoff for thermophotovoltaic energy conversion
Authors: Ni Qing , Ramesh Rajagopalan , Chen Cheng-An , Wang Liping 
Published: June 2021

A semiconductor emitter can possibly achieve a sharp cutoff wavelength due to its intrinsic bandgap absorption and almost zero sub-bandgap emission without doping. A germanium-wafer-based selective emitter with front-side antireflection and backside metal coating is studied here for thermophotovoltaic (TPV) energy conversion. Optical simulation predicts the spectral emittance above 0.9 in the wavelengths from 1 to 1.85 µm and below 0.2 in the sub-bandgap range with a sharp cutoff around the bandgap, indicating superior spectral selectivity behavior. This is confirmed by excellent agreement with indirectly measured spectral emittance of the fabricated Ge-based selective emitter sample. Furthermore, the TPV efficiency by pairing the Ge-based selective emitter with a GaSb cell is theoretically analyzed at different temperatures. This Letter facilitates the development of the semiconductor-based selective emitters for enhancing TPV performance.
Eu3+-doped Bi2MoO6 phosphors for highly sensitive and visible H2S gas indicator material: optical and color properties, and gas sensing performance
Authors: Kang Tae Wook , Park Young Ji , Jeong Gyu Jin , Bae Byungseo , Kim Sun Woog 
Published: June 2021

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Prevalence of coronary risk factors in contemporary practice among patients undergoing their first percutaneous coronary intervention: Implications for primary prevention
Authors: Gurm Zoya , Seth Milan , Daher Edouard , Pielsticker Elizabeth , Qureshi M. Imran , Zainea Mark , Tucciarone Michael , Hanzel George , Henke Peter K. , Sukul Devraj 
Published: June 2021

Background
Cigarette smoking, hypertension, dyslipidemia, diabetes, and obesity are conventional risk factors (RFs) for coronary artery disease (CAD). Population trends for these RFs have varied in recent decades. Consequently, the risk factor profile for patients presenting with a new diagnosis of CAD in contemporary practice remains unknown.

Objectives
To examine the prevalence of RFs and their temporal trends among patients without a history of myocardial infarction or revascularization who underwent their first percutaneous coronary intervention (PCI).

Methods
We examined the prevalence and temporal trends of RFs among patients without a history of prior myocardial infarction, PCI, or coronary artery bypass graft surgery who underwent PCI at 47 non-federal hospitals in Michigan between 1/1/2010 and 3/31/2018.

Results
Of 69,571 men and 38,930 women in the study cohort, 95.5% of patients had 1 or more RFs and nearly half (55.2% of women and 48.7% of men) had ≥3 RFs. The gap in the mean age at the time of presentation between men and women narrowed as the number of RFs increased with a gap of 6 years among those with 2 RFs to <1 year among those with 5 RFs. Compared with patients without a current/recent history of smoking, those with a current/recent history of smoking presented a decade earlier (age 56.8 versus 66.9 years; p <0.0001). Compared with patients without obesity, patients with obesity presented 4.0 years earlier (age 61.4 years versus 65.4 years; p <0.0001).

Conclusions
Modifiable RFs are widely prevalent among patients undergoing their first PCI. Smoking and obesity are associated with an earlier age of presentation. Population-level interventions aimed at preventing obesity and smoking could significantly delay the onset of CAD and the need for PCI.
Lack of efficacy of hydroxychloroquine and azithromycin in patients hospitalized for COVID-19 pneumonia: A retrospective study
Authors: Saib Anis , Amara Walid , Wang Pascal , Cattan Simon , Dellal Azeddine , Regaieg Kais , Nahon Stephane , Nallet Olivier , Nguyen Lee S. 
Published: June 2021

Background
Hydroxychloroquine combined with azithromycin (HCQ/AZI) has initially been used against coronavirus disease-2019 (COVID-19). In this retrospective study, we assessed the clinical effects of HCQ/AZI, with a 28-days follow-up.

Methods
In a registry-study which included patients hospitalized for COVID-19 between March 15 and April 2, 2020, we compared patients who received HCQ/AZI to those who did not, regarding a composite outcome of mortality and mechanical ventilation with a 28-days follow-up. QT was monitored for patients treated with HCQ/AZI. Were excluded patients in intensive care units, palliative care and ventilated within 24 hours of admission. Three analyses were performed to adjust for selection bias: propensity score matching, multivariable survival, and inverse probability score weighting (IPSW) analyses.

Results
Overall, 203 patients were included: 60 patients treated by HCQ/AZI and 143 control patients. During the 28-days follow-up, 32 (16.3%) patients presented the primary outcome and 23 (12.3%) patients died. Propensity-score matching identified 52 unique pairs of patients with similar characteristics. In the matched cohort (n = 104), HCQ/AZI was not associated with the primary composite outcome (log-rank p-value = 0.16). In the overall cohort (n = 203), survival and IPSW analyses also found no benefit from HCQ/AZI. In the HCQ/AZI group, 11 (18.3%) patients prolonged QT interval duration, requiring treatment cessation.

Conclusions
HCQ/AZI combination therapy was not associated with lower in-hospital mortality and mechanical ventilation rate, with a 28-days follow-up. In the HCQ/AZI group, 18.3% of patients presented a prolonged QT interval requiring treatment cessation, however, control group was not monitored for this adverse event, making comparison impossible.