bluuebunny/crossref_metadata_2025_split huggingface dataset:
An epitope specific patient-derived LGI1-autoantibody enhances neuronal excitability by modulating the Kv1.1 channel
Authors: Extrémet Johanna , El Far Oussama , Irani Sarosh R , Debanne Dominique , Russier Michael 
Published: December 2021

AbstractLeucine-rich Glioma Inactivated protein 1 (LGI1) is expressed in the central nervous and genetic loss of function is associated with epileptic disorders. Also, patients with LGI1-directed autoantibodies have frequent focal seizures as a key feature of their disease. LGI1 is composed of a Leucine Rich Repeat (LRR) and an Epitempin (EPTP) domain. These domains are reported to interact with different aspects of the transsynaptic complex formed by LGI1 at excitatory synapses, including presynaptic Kv1 potassium channels. Patient-derived monoclonal antibodies (mAbs) are ideal reagents to study whether domain-specific LGI1-autoantibodies induce epileptiform activities in neurons, and their downstream mechanisms. To address this question, we measured the intrinsic excitability of CA3 pyramidal neurons in organotypic cultures from rat hippocampus treated with either a LRR- or an EPTP-reactive patient-derived mAb. The antibodies induced changes in neuronal intrinsic excitability which led us to measure their effects on Kv1-type potassium currents. We found an increase of intrinsic excitability correlated with a reduction of the sensitivity to a selective Kv1.1-channel blocker in neurons treated with the LRR mAb compared to the control, but not in neurons treated with the EPTP mAb. Our findings suggest LRR mAbs are able to modulate neuronal excitability that could account for epileptiform activities observed in patients.
Predicting genes associated with RNA methylation pathways using machine learning
Authors: Tsagkogeorga Georgia , Santos-Rosa Helena , Alendar Andrej , Leggate Dan , Rausch Oliver , Kouzarides Tony , Weisser Hendrik , Han Namshik 
Published: December 2021

ABSTRACTRNA methylation plays an important role in functional regulation of RNAs, and has thus attracted an increasing interest in biology and drug discovery. Here, we collected and collated transcriptomic, proteomic, structural and physical interaction data from the Harmonizome database, and applied supervised machine learning to predict novel genes associated with RNA methylation pathways in human. We selected five types of classifiers, which we trained and evaluated using cross-validation on multiple training sets. The best models reached 88% accuracy based on cross-validation, and an average 91% accuracy on the test set. Using protein-protein interaction data, we propose six molecular sub-networks linking model predictions to previously known RNA methylation genes, with roles in mRNA methylation, tRNA processing, rRNA processing, but also protein and chromatin modifications. Our study exemplifies how access to large omics datasets joined by machine learning methods can be used to predict gene function.
A nanobody-based toolset to monitor and modify the mitochondrial GTPase Miro1
Authors: Fagbadebo Funmilayo O. , Kaiser Philipp D. , Zittlau Katharina , Bartlick Natascha , Wagner Teresa R. , Froehlich Theresa , Jarjour Grace , Nueske Stefan , Scholz Armin , Traenkle Bjoern , Macek Boris , Rothbauer Ulrich 
Published: December 2021

AbstractThe mitochondrial outer membrane (MOM)-anchored GTPase Miro1, is a central player in mitochondrial transport and homeostasis. The dysregulation of Miro1 in amyotrophic lateral sclerosis (ALS) and Parkinson’s disease (PD) suggests that Miro1 may be a potential biomarker or drug target in neuronal disorders. However, the molecular functionality of Miro1 under (patho-) physiological conditions is poorly known. For a more comprehensive understanding of the molecular functions of Miro1, we have developed Miro1-specific nanobodies (Nbs) as novel research tools. We identified seven Nbs that bind either the N- or C-terminal GTPase domain of Miro1 and demonstrate their application as research tools for proteomic and imaging approaches. To visualize the dynamics of Miro1 in real time, we selected intracellularly functional Nbs, which we reformatted into chromobodies (Cbs) for time- lapse imaging of Miro1. By genetic fusion to an Fbox domain, these Nbs were further converted into Miro1-specific degrons and applied for targeted degradation of Miro1 in live cells. In summary, this study presents a collection of novel Nbs that serve as a toolkit for advanced biochemical and intracellular studies and modulations of Miro1, thereby contributing to the understanding of the functional role of Miro1 in disease-derived model systems.
Tomato SlBES1.8 influences leaf morphogenesis by mediating gibberellin metabolism and signaling
Authors: Su Deding , Xiang Wei , Liang Qin , Wen Ling , Shi Yuan , Liu Yudong , Xian Zhiqiang , Li Zhengguo 
Published: December 2021

AbstractLeaf morphogenetic activity determines its shape diversity. However, our knowledge to the regulatory mechanism in maintaining leaf morphogenetic capacity is still limited. In tomato, gibberellin (GA) negatively regulates leaf complexity by shortening the morphogenetic window. We here reported a tomato BRI1-EMS-SUPPRESSOR 1 (BES1) transcription factor, SlBES1.8, that promoted the simplification of leaf pattern in a similar manner as GA functions. Enhanced level of SlBES1.8 dramatically decreased the sensibility of tomato to GA whereas increased the sensibility to the GA biosynthesis inhibitor, PAC. In line with the phenotypic observation, the endogenous bioactive GA contents were increased in OE-SlBES1.8 lines, which certainly promoted the degradation of the GA signaling negative regulator, SlDELLA. Moreover, transcriptomic analysis uncovered a set of overlapping genomic targets of SlBES1.8 and GA, and most of them were regulated in the same way. Expression studies showed the repression of SlBES1.8 to the transcriptions of two GA deactivated genes, SlGA2ox2 and SlGA2ox6, and one GA receptor, SlGID1b-1. Further experiments confirmed the direct regulation of SlBES1.8 to their promoters. On the other hand, SlDELLA physically interacted with SlBES1.8 and further inhibited its transcriptional regulation activity by abolishing SlBES1.8-DNA binding. Conclusively, by mediating GA deactivation and signaling, SlBES1.8 greatly influenced tomato leaf morphogenesis.HighlightThe BRI1-EMS-SUPPRESSOR 1 (BES1) family protein SlBES1.8 promotes leaf simplification by inhibiting gibberellin deactivation and physically interacting with DELLA protein in tomato.
Credit-linked insurance bundles in Zambia: evidence from the aftermath of a shock
Authors: Van Asseldonk Marcel , Onumah Gideon , Lensink Robert 
Published: December 2021

PurposeThis research seeks to assess the impact of a credit-linked insurance bundle in Zambia, in terms of the inputs used and the amount of maize subsequently produced and sold.Design/methodology/approachTo estimate the impact of a credit-linked insurance bundle, this research relies on a natural field experiment. A cross-sectional survey, conducted among 409 households that enrolled in a credit-linked insurance program prior to a drought and adverse market conditions, revealed that 252 households dropped out of the program. Of these, 113 households left for an exogenous, involuntary reason (i.e. group loan was not repaid on time).FindingsA comparison of households that used the program and those that dropped out reveals that smallholders who lost the credit-linked insurance bundle purchased less fertilizer (−36%), and this input restriction resulted in diminished harvests (−27%) and less quantity sold (−31%).Research limitations/implicationsRisk-exposed smallholders tend to be severely credit constrained, so they cannot invest in sufficient inputs to increase their yields. A credit-linked insurance scheme provides such risk-exposed smallholders, who lack or have only limited collateral, with commercial agricultural credit services and greater access to input. The current analysis cannot specifically attribute the impact of individual components of the bundle (i.e. credit, insurance and input supply), but the overall impact is substantial. The implication of this research is that policy initiatives which support a credit-linked insurance system should not only encourage upscaling but also resilience of the scheme.Practical implicationsAligning government and public support programs with private initiatives suggests opportunities for greater benefits.Social implicationsAfrican farmers are mainly smallholders, so they face inherent production risks. They also tend to be severely credit constrained, with few means to mitigate these risks, so they suffer from a limited capacity to invest in improved farm technology systems that might increase their productivity. Insured input bundles could help farmers to cope better with adverse risks and facilitate increase productivity.Originality/valueThis research design exploits the peculiarity of the data, including group liability and a strict time window for loan repayments to remain eligible in a forthcoming growing season. This impact assessment approach is rigorous in controlling for self-selection bias and thus offers opportunities to establish how households in each sub-sample (eligible or not) are (un)able to ride out a lean season, following a drought and adverse market conditions.
Administrative and legal mechanisms for combating corruption
Authors: Lytvyn Nataliia A. , Artemenko Olena V. , Kovalova Svitlana S. , Kobets Maryna P. , Kashtan (Grygorieva) Elena V. 
Published: December 2021

Purpose
The purpose of this paper is to study the administrative and legal mechanisms of combating corruption, namely, to determine the means by which it is possible to develop a positive experience in the fight against corruption.

Design/methodology/approach
Among the methods used to study the problems of the stated subject, the dialectical, comparative-legal, systems, historical and legal, formal and legal, analysis and synthesis can be distinguished.

Findings
The authors investigated the experience of foreign countries in combating corruption and suggested implementing international experience in national legislation for the successful fight against corruption. In the course of the study, the current state of legal regulations governing anti-corruption activities was characterised, corruption and the main reasons for committing corruption acts were investigated, the problems that arise in the fight against corruption were identified, the main administrative and legal mechanisms for combating corruption were established and the effectiveness of applying these mechanisms in practice was studied.

Practical implications
The provisions that are enshrined in this paper are of practical value for individuals whose activities are aimed at fighting corruption, as Ukraine is one of the states where corruption flourishes and where the fight against corruption has not been directly implemented in practice for many years.

Originality/value
Based on the example of international experience in the fight against corruption, proposals and recommendations for improving administrative and legal mechanisms for combating corruption have been developed.
The problem of CSR: an exploration of relationship between CSR initiatives and excess work ethic
Authors: Ali Muhammad 
Published: December 2021

Purpose
The purpose of this study is to bring to light the downside of the positive effects of corporate social responsibility (CSR) concerning employee work practices. The focus is on the aspects of excess work ethic (EWE) that are hypothesized as the result of increased organizational identification (OI) due to CSR engagement. This excessive involvement by employees in their work results in neglect of their personal lives.

Design/methodology/approach
The sample has been taken from employees of various organizational sectors in Pakistan. Structural equation modeling is used to analyze the hypothesized relationships among the variables in the conceptual framework.

Findings
The findings of the study reveal that CSR perception of employees negates the excessive work ethic but the introduction of a higher level of OI due to work meaningfulness indirectly positively affects EWE with pro-environmental orientation (PEO) as a moderator.

Originality/value
The relationship between CSR and variables such as work engagement has been explored in the recent literature but the heightened level of OI indulging the employees into overwork is rarely been explored. Also, the use of PEO as a mediator adds to the knowledge on the subject.
Not a token! A discussion on racial capitalism and its impact on academic librarians and libraries
Authors: Vong Silvia 
Published: December 2021

PurposeThe purpose of this article is to introduce the concept of racial capitalism in the context of academic libraries.Design/methodology/approachThis paper draws on Leong's (2013) extended theory of racial capitalism and identifies how neoliberalism and racial capitalism are tied as well as how it is manifested in academic libraries through tokenism, racialized tasks, consuming racial trauma, cultural performance demands, workload demands and pay inequity.FindingsThe article ends with some suggestions in how to address these problematic practices though dismantling meritocratic systems, critical race theory in LIS education and training, and funding EDI work.Originality/valueThe article explores a concept in the academic library context and points to practices and structures that may commodify racialized identities.
Boards of a Feather: Homophily in Foreign Director Appointments Around the World
Authors: BARRIOS JOHN M. , BIANCHI PIETRO A. , ISIDRO HELENA , NANDA DHANANJAY 
Published: December 2021

ABSTRACTWe examine how similarity in institutional, legal, and social characteristics between a firm's and its directors’ home countries, that is, country‐pair homophily, affects foreign director appointments. We estimate a gravity model that includes economic and geographic proximity and find that country‐pair homophily is a significant determinant of foreign director appointments to corporate boards. We also find that country‐pair homophily limits the appointments of foreign directors from high‐quality governance countries to firms located in low‐quality governance countries, which may reduce the role of board internationalization in promoting the global convergence of governance practices. We analyze changes in foreign director appointments around the international adoption of IFRS and Norway's gender‐quota rule and find a higher appointment likelihood for directors originating from countries that are institutionally and culturally similar to that of the firm. Our findings point to the critical role that country‐pair homophily plays in matching director to boards with implications for the diffusion of governance practices globally.
The ZAX Herbivory Trainer—Free software for training researchers to visually estimate leaf damage
Authors: Xirocostas Zoe A. , Debono Samuel A. , Slavich Eve , Moles Angela T. 
Published: December 2021

Abstract

Plants lose a remarkable amount of energy to herbivorous animals, and this damage has substantial impacts on plant fitness and species' distributions. There are many ways ecologists can measure leaf damage, with some methods being more time‐consuming than others. Due to a high variance in herbivory, accurate quantification of damage at the population level requires sampling of many leaves. A simple yet effective solution to this problem is to estimate leaf damage visually.

Visually estimating leaf damage may be less accurate than scanning methods, but visual estimates of leaf damage are much faster than digital measurements. Using simulations, we show that gathering larger quantities of data at a slightly higher level of inaccuracy gives a more accurate estimate of a population's overall leaf damage than fewer, exact measurements.

We then introduce the ZAX Herbivory Trainer, a free online application that teaches researchers to accurately visually estimate leaf damage. On average, users took ~9 min and 48 images to complete our trainer which significantly decreased their estimate inaccuracy from 13.2% to 6%. This low level of inaccuracy can be retained up to 3 months post‐training so researchers can use the ZAX Herbivory Trainer once prior to short fieldwork or every 3 months for extensive fieldwork. We also recommend a cut‐off point, whereby if a person has not completed the app in 17.5 min or 85 images (90th percentile), they may not be suitable to estimate herbivory for research purposes.

The ZAX Herbivory Trainer will allow researchers of any experience level to assess herbivory quickly and accurately in a globally standardised way. International collaborators, students and citizen scientists can all find use in this app, no matter the scale of their projects. From this we can gather better data to address big picture questions in ecology such as patterns in herbivory relating to latitude or climate change.
CFTR mutational screening by next‐generation sequencing reveals novel variants and a high carrier rate in a Middle Eastern population
Authors: Farra Chantal , Awwad Johnny , Hamadeh Lama , Khoueiry Pierre , Halawi Zeina , Yazbeck Nadine , Daher Rose , Souaid Mirna , Hamdar Layal , Yammine Tony , Yunis Khalid 
Published: December 2021

AbstractCystic fibrosis is the most common life‐limiting autosomal recessive disease in western countries with an incidence of 1:2500 in United States and 1:1000 in some European countries. Similar incidences were noted for the Middle East with variations from 1 in 2560 to 1 in 15,876 according to the degree of consanguinity. This is a preliminary systematic study that aims to assess the incidence and carrier rate of cystic fibrosis in the Middle Eastern Lebanese population; known for a high frequency of consanguinity. One hundred thirteen DNA samples were collected from neonatal blood cards obtained from newborns to healthy unrelated families with no previous history of Cystic fibrosis. Screening for Cystic Fibrosis‐causing pathogenic variants was performed using next generation sequencing, and 17 different single nucleotide variants were detected, including six pathogenic and likely pathogenic. 5.5%–7% newborns were found to be carriers of a variant strongly suggestive of pathogenicity and comparable to published literature worldwide. This pilot analysis highlights the challenging interpretation of CFTR variants in a country underrepresented by large ethnic population analyses, and stresses the importance of premarital screening programs for Cystic fibrosis.
Incidence of Clostridioides difficile in patients post loop ileostomy reversal in an Australian tertiary hospital: a retrospective study
Authors: Jordan Stephanie , Hui Nathan , Doudle Mark , Von Papen Michael , Naik Arun , Lu Cu Tai , Nolan Gregory , Cooper Michelle 
Published: December 2021

AbstractIntroductionThe purpose of a loop ileostomy is to temporarily divert faeces away from a distal anastomosis, to reduce the consequences of anastomotic leak. This ultimately requires a second procedure to restore bowel continuity, which confers risk of complications including the development of Clostridioides difficile infection (CDI). It is hypothesized that patients who undergo loop ileostomy reversal are at increased risk of CDI when compared with other patients undergoing elective colorectal surgical procedures, and that these patients also experience an increased length of stay (LOS).MethodsA retrospective cohort study was performed on all patients who underwent loop ileostomy reversal at the Gold Coast Hospital and Health Service between 1 January 2012 and 31 December 2019.ResultsTwo hundred and twenty‐eight patients were identified. Eight tested positive for CDI on faecal PCR (3.51%), a higher incidence than that in patients who underwent an elective colorectal surgical procedure during the same period (0.83%) (RR = 4.23). Additionally, median LOS for ileostomy reversal patients was significantly increased in those who also had CDI when compared with those without CDI (11 versus 4 days; P = 0.0003).ConclusionThe study confirmed that the incidence of CDI was higher in those who underwent ileostomy reversal when compared with an otherwise comparable hospital population (elective colorectal surgery patients). Additionally, those patients who underwent ileostomy reversal and had CDI experienced an increased LOS which translates to increased cost to the healthcare system. Further investigation into pre‐operative screening and prophylactic antibiotics should be considered as a measure to mitigate this post‐operative complication.
The development of uncertainty monitoring during kindergarten: Change and longitudinal relations with executive function and vocabulary in children from low‐income backgrounds
Authors: Gonzales Christopher R. , Merculief Alexis , McClelland Megan M. , Ghetti Simona 
Published: December 2021

AbstractChildren's ability to monitor subjective feelings of uncertainty (i.e., engage in uncertainty monitoring) is a central metacognitive skill. In the current study, we examined the development of uncertainty monitoring as well as its relations with vocabulary and executive function development in children (N = 137, 52% female) from predominately White and Latinx/Hispanic backgrounds when they were 4–6 years old and enrolled in a Head Start preschool and kindergarten between 2018 and 2019. We found that children's uncertainty monitoring improved during the kindergarten year. Children's executive function and vocabulary in preschool and vocabulary growth from preschool to kindergarten predicted uncertainty monitoring at the end of kindergarten, which sheds new light on potential mechanisms supporting children's metacognitive development.
Embodying children’s rights in Italy and Spain: Unmasking some elements of the cultural politics of childhood
Authors: Grau‐Grau Marc , Tracchi Matteo , Panebianco Daria , Kisunaite Aida 
Published: December 2021

AbstractThis study explores how some aspects of the cultural politics of childhood in Italy and Spain limit, allow or encourage the fuller realisation of the UN Convention on the Rights of the Child, using the complementary voices of 49 participants, including policymakers, leaders of third sector organisations and families in vulnerable situations. Our research contributes to the advancement of the field bycontextualisingsome of the facilitating and challenging factors regarding the enforcement of children's rights, divided into three different levels of analysis: structural factors, political factors and cultural factors.