bluuebunny/crossref_metadata_2025_split huggingface dataset:
Incidence of Symptomatic COVID-19 in Close Contacts of Patients After Discharge From Hospital
Authors: Ahmadi Ayat , Poopak Amirhossein , Nazemi Sina , Mohammadi Negin , Eslami Bita , Seyyedsalehi Monireh Sadat , Doshmangir Leila , Allameh Seyed Farshad , Zendehdel Kazem 
Published: July 2021

Abstract
Background: There is a little evidence about the infectiousness of recovered COVID-19 patients. Considering that the circumstance of the isolation of the COVID-19 patients after-discharge is not always optimal, it is not very unlikely that viral transmission still occurs after hospital discharge. This study aims to investigate incidence of symptomatic COVID-19 in close contacts of recovered patients after discharge from hospitalMethods: Four hundred fifty discharged COVID-19 patients discharged from the largest public treatment center in Tehran, capital city of Iran were followed up. Demographic and clinical data of participants were collected from medical records. Follow-up data were acquired via telephone call interviews with patients or their main caregivers at home. Results: The study's response rate was 93.77%(422 participated in the study). 257 patients were male (60.90%) and 165(39.10%) were female (sex ratio =1.55 male). The most prevalent comorbidities in these patients were hypertension (n=111, 29.68%) and diabetes (n=93, 24.80%). The mean±sd of home isolation after discharge was 25.85±23.78. Forty-one (9.71%) patients had at least one new case in their close contacts up to three weeks since they discharged. There was a significant association between having at least a comorbidity with the odds of getting infected in close contacts [OR(CI): 2.45 (1.05 – 4.72); P: .03]. Density of inhabitant per houses rooms and the quality of isolation had significant associations with observing new cases in the patients' close contacts [high to moderate; OR(CI): 2.44 (1.06 – 5.61); P: .03], [ bad to good; OR(CI): 2.31 (1.17 – 4.59); P: .01], respectively.Conclusion: After hospital discharge, COVID-19 transmission can still occur, when number of people lives together in a single house. An explanation can be that the less precaution measures are taken by recovered patients’ cohabitants. Such condition is more likely to happen when the recovered patient has other chronic diseases.
Significance of Preoperative Left Ventricular Ejection Fraction in 5-year Outcome After Isolated CABG
Authors: Fallahzadeh Aida , Sheikhy Ali , Ajam Ali , Sadeghian Saeed , Pashang Mina , Shirzad Mahmoud , Bagheri Jamshid , Mansourian Soheil , Momtahen Shahram , Hosseini Kaveh 
Published: July 2021

Abstract
Background: Pre-operative ejection fraction (EF) and comorbidities affect post-op outcomes. We aimed to compare the mortality and adverse events of patients with different baseline EF and also to evaluate the distribution of comorbidities in each EF group. Methods: A total of 20,937 patients who underwent isolated coronary artery bypass graft (CABG) surgery from January 2006 to December 2016 was included. Patients were divided into three groups based on their pre-operative left ventricular EF as follows; 1) Normal: EF ≥ 50%; 2) Mild to moderately reduced: 50% < EF ≤ 35%; and 3) Severely reduced: EF< 35%. The backward elimination method was considered for multivariate Cox-regression analysis to locate predictors of mortality and non-fatal cerebro-cardiovascular events (CCVEs). The median follow-up time was 5.61 [3.12- 8.0] years. Results: The mean age in the total population was 60.94 ± 9.51 years and 73.6% of the total population was male. Diabetes mellitus was the common risk factor of mortality and CCVE in all EF groups. Impaired renal function (GFR<60 ml/min) was associated with a higher risk of mortality after CABG regardless of EF level. The median 5-year mortality rate in patients with normal EF, mild-moderately reduced EF and severely reduced EF were 9.5%, 12.8%, and 22.7% respectively (P< 0.001). Although the trend of CCVEs was higher in severe left ventricle (LV) dysfunction, it was not statistically significant (p = 0.071). Conclusion: Patients with severely reduced EF are at higher risk of mortality after CABG compared to those with higher EF levels; however, the rate of CCVEs may not be necessarily higher after adjustment for multiple pre-operative comorbidities.
MBNL1 Regulates the Expression and Alternative Splicing of Genes Enriched in Cell Adhesion and Apoptosis
Authors: Liu Meijia , Song Xianjing , Li Longbo , Song Chunli , Shi Yongfeng 
Published: July 2021

Abstract
Muscleblind Like Splicing Regulator 1 (MBNL1), one canonical RNA binding protein (RBP), plays important roles in the regulation of the alternative splicing (AS) on pre-mRNAs. MBNL1 has traditionally been considered involved in the pathogenesis of myotonic dystrophy. Recent researches point out that MBNL1 has an effect on cancer progress, but the underlying mechanisms are unclear. In this study, we obtained the regulated transcriptome profile of MBNL1 in HeLa cells by RNA-seq analysis. The results showed that the knockdown of MBNL1 promoted cell proliferation while inhibited apoptosis. We found 398 genes were differentially up-regulated and 277 down-regulated by MBNL1 knockdown (KD). The differentially expressed genes (DEGs) regulated by MBNL1-KD were enriched in the signal pathways of homophilic cell adhesion, apoptotic process, extracellular matrix organization and cell migration. Systematical AS analysis revealed 504 MBNL1-regulated AS events. The regulated alternative splicing genes (RASGs) were enriched in the signal pathways of apoptotic signaling pathway, positive regulation of apoptotic process, adherent junction, fatty acid elongation and DNA repair. In summary, our results demonstrate that the knockdown of MBNL1 have significant effects on cell proliferation and apoptosis by regulating the expression and alternative splicing of associated genes, illustrating the possible molecular mechanisms of MBNL1 in cancer pathogenesis and progression and other diseases.
A Study on the Optoelectronics Parameters of Natural Dyes Extracted From Beetroot, Cabbage, Walnut and Henna For Potential Applications in Organic Electronics
Authors: Amin Peshawa O. , Muhammadsharif Fahmi F. , Saeed Salah Raza , Sulaiman Khaulah 
Published: July 2021

Abstract
In this work, the optoelectronic parameters of natural dyes extracted from beetroot, red cabbage, walnut leaves, and henna are determined and investigated. These include optical energy gap (Eg), extinction coefficient (k), refractive index (n), dielectric constant ( εr ) and optical conductivity ( σr ). Results showed that cabbage dye exhibited a maximum values of n=6.08, εr = 37.18 and σr = 13.92 * 10-3S/cm, while henna dye presented the lowest values of n = 1.19, εr = 1.5 and σr = 0.039 * 10-3S/cm compared to the optoelectronic parameters of the other studied samples. The optical absorption in the dyes were found to obey a direct allowed transition between the molecular energy levels. The results presented here can be of a special importance for some potential applications in organic electronics such as organic photovoltaics and sensors.
Multivariate Diversity Analysis of Quantitative Traits of Mung Bean [Vigna radiata (L.) Wilczek] Genotypes
Authors: Yoseph Tekle , Mekbib Firew , Amsalu Berhanu , Tadele Zerihun 
Published: July 2021

Abstract
Mung bean is an important pulse crop grown by poor farmers in marginal and drought-prone areas of Ethiopia. Information on the extent of genetic divergence in mung bean is vital to identify diverse genotypes for crop improvement and the efficient utilization of the existing genetic resources. Therefore, the objectives of the study were to assess the extent and pattern of morphological diversity among the mung bean genotypes and to identify the traits contributing to the genetic diversity using multivariate analyses. The experiment was conducted using 60 mung bean genotypes at Jinka Agricultural Research Center during the 2018 cropping season. The first seven principal components explained 80.1% of the total variation. Almost all the studied traits were important contributors to the divergence. The cluster analysis based on quantitative traits revealed four distinct groups. The highest inter-cluster distance was recorded between cluster I and cluster IV (D 2  = 43.16 units). The minimum inter-cluster distance was noted between cluster III and cluster IV (D2 = 12.16 units). The maximum and minimum intra-cluster distances D2 were recorded within cluster I (D2 = 6.49 units) and cluster III (D2 = 3.53 units), respectively). The range of intra and inter-cluster distance was 3.53 to 6.49 units and 12.16 to 43.16 units, respectively. Hence, the high genetic distance exhibited within and among clusters has to be exploited through the crossing and selection of the most divergent parents for future mung bean breeding programs.
Deformation of the nucleus by TGFβ1 via the remodeling of nuclear envelope and histone isoforms
Authors: Chi Ya-Hui , Wang Wan-Ping , Hung Ming-Chun , Liou Gunn-Guang , Wang Jing-Ya , Chao Pen-Hsiu Grace 
Published: July 2021

Abstract
The cause of nuclear shape abnormalities which are often seen in pre-neoplastic and malignant tissues is not clear. In this study we report that deformation of the nucleus can be induced by TGFb1 stimulation in several cell lines including Huh7. In our results, the upregulated histone H3.3 expression downstream of SMAD signaling contributed to TGFb1-induced nuclear deformation, a process of which requires incorporation of the nuclear envelope (NE) proteins lamin B1 and SUN1. During this process, the NE constitutively ruptured and reformed with no observable indications of DNA damage response. Contrast to lamin B1 which was relatively stationary around the nucleus, the upregulated lamin A was highly mobile, shuttling between the nucleus and cytoplasm, and clustering at the nuclear periphery. The chromatin regions that lost NE coverage formed a supra-nucleosomal structure characterized by elevated histone H3K27me3 and histone H1, the formation of which depended on the presence of lamin A. These results provide evidence that shape of the nucleus can be modulated through TGFb1-induced compositional changes in the chromatin and nuclear lamina.
3D hESC exosomes enriched with miR-6766-3p ameliorates liver fibrosis by attenuating activated stellate cells through targeting the TGFβRII-SMADS pathway
Authors: Wang Ning , Li Xiajing , Zhong Zhiyong , Qiu Yaqi , Liu Shoupei , Wu Haibin , Tang Xianglian , Chen Chuxin , Fu Yingjie , Chen Qicong , Guo Tingting , Li Jinsong , Zhang Shuai , Zern Mark A , Ma Keqing , Wang Bailin , Ou Yimeng , Gu Weili , Cao Jie , Chen Honglin , Duan Yuyou 
Published: July 2021

Abstract
BackgroundExosomes secreted from stem cells exerted salutary effects on the fibrotic liver. Herein, the roles of exosomes derived from human embryonic stem cell (hESC) in anti-fibrosis were extensively investigated. Compared with two-dimensional (2D) culture, the clinical and biological relevance of three-dimensional (3D) cell spheroids were greater because of their higher regeneration potential since they behave more like cells in vivo. In our study, exosomes derived from 3D human embryonic stem cells (hESC) spheroids and the monolayer (2D) hESCs were collected and compared the therapeutic potential for fibrotic liver in vitro and in vivo. ResultsIn vitro, PKH26 labled-hESC-Exosomes were shown to be internalized and integrated into TGFβ-activated-LX2 cells, and reduced the expression of profibrogenic markers, thereby regulating cellular phenotypes. TPEF imaging indicated that PKH26-labled-3D-hESC-Exsomes possessed an enhanced capacity to accumulate in the livers and exhibited more dramatic therapeutic potential in the injured livers of fibrosis mouse model. 3D-hESC-Exosomes decreased profibrogenic markers and liver injury markers, and improved the level of liver functioning proteins, eventually restoring liver function of fibrosis mice. miRNA array revealed a significant enrichment of miR-6766-3p in 3D-hESC-Exosomes, moreover, bioinformatics and dual luciferase reporter assay identified and confirmed the TGFβRII gene as the target of miR-6766-3p. Furthermore, the delivery of miR-6766-3p into activated-LX2 cells decreased cell proliferation, chemotaxis and profibrotic effects, and further investigation demonstrated that the expression of target gene TGFβRII and its downstream SMADs proteins, especially phosphorylated protein p-SMAD2/3 was also notably down-regulated by miR-6766-3p. These findings unveiled that miR-6766-3p in 3D-hESC-Exosomes inactivated SMADs signaling by inhibiting TGFβRII expression, consequently attenuating stellate cell activation and suppressing liver fibrosis. ConclusionsOur results showed that miR-6766-3p in the 3D-hESC-Exosomes inactivates smads signaling by restraining TGFβRII expression, attenuated LX2 cell activation and suppressed liver fibrosis, suggesting that 3D-hESC-Exosome enriched-miR6766-3p is a novel anti-fibrotic therapeutics for treating chronic liver disease. These results also proposed a significant strategy that 3D-Exo could be used as natural nanoparticles to rescue liver injury via delivering antifibrotic miR-6766-3p.
Periodic oscillations for a graphene-based electrostatic micro actuator
Authors: Nuñez Daniel Elias , Murcia Larry , Galan Jorge 
Published: July 2021

Abstract
We study the mechanical oscillations for a novel model of a graphene-based electrostatic parallel plates micro actuator introduced by Wei et al.(2017), considering damping effects when a periodic voltage with alternating current is applied. Our analysis starts from recent results about this MEMS model with constant voltage, and provides new insights on the periodic mechanical responses for a variable input voltage. We derive sufficient conditions on the system physical components for which periodic oscillations with constant sign exist together with their stability properties. Specifically, under some conditions, the existence of three periodic solutions is established, one of them is negative and the others are positive in sign. The positive one nearby the origin is asymptotically locally stable, whilst the other two are unstable. Additionally, we prove that no further constant sign periodic solutions can be found. The existence of periodic solutions is approached from direct and reverse order Lower and Upper Solutions Method, and the stability assertions are derived from the Liapounoff-Zukovskii criteria for Hill's equations and the linearization principle. Theoretical results are complemented by numerical simulations and numerical continuation results. Furthermore, these numerical simulations evidence the robustness of the graphene-based MEMS model over the traditional ones.
Composition of Mosquito Fauna and Insecticide Resistance Status of Anopheles gambiae complex in Itang special woreda, Gambella, Southwestern Ethiopia
Authors: Chanyalew Tebiban , Yewhalaw Delenasaw , Simma Eba Alemayehu 
Published: July 2021

Abstract
IntroductionMalaria is a leading cause of morbidity and mortality mainly in sub-Saharan African countries. Plasmodium falciparum and P. vivax are the dominant malaria parasites responsible for the majority of malaria cases in Africa. The aim of this study was to investigate composition of mosquito fauna and insecticide resistance status of Anopheles mosquito in Itang special woreda (district), Gambella, southwestern Ethiopia.Materials and methodsAdult mosquitoes were sampled from September 2020 to Feburary 2021 using Centers for Disease Control and Prevention (CDC) light trap and Pyrethrum Spray Catch (PSC). Moreover, mosquito larvae were collected from different breeding sites and reared to adults. Susceptibility tests were conducted on adult two to three days old non blood fed female Anopheles gambiae s.l following world health organization (WHO) standard susceptibility test procedure. Insecticide impregnated papers with deltamethrin (0.05%), alpha-cypermethrin (0.05%), propoxur (0.1%), pirimiphos-methyl (0.25%) and bendiocarb (0.1%) were used to assess susceptibility status of Anopheles gambiae s.l populations in the study area. Moreover, molecular diagnostics were done for the identification of member species of Anopheles gambiae s.l and detection of knockdown resistance (kdr) using species specific polymerase chain reaction (PCR) and allele specific PCR.ResultsIn total, 468 adult mosquitoes were collected from different houses. Culex mosquitoes were the most dominant (80.4%) followed by Anopheles mosquitoes. Three species of Anopheles mosquitoes (An. coustani, An. pharoensis, and An. gambiae (s.l.)) were identified, of which An. coustani was the dominant (8.1%) species. Out of 468 adult mosquitoes, 294 were blood fed while 46 were half-gravid and gravid. The WHO bioassay tests revealed that the populations of An. gambiae s.l in the study area are resistant against alpha-cypermethrin and deltamethrin whereas, susceptible to bendiocarb, pirimiphos-methyl and propoxur. Out of the total 86 An. gambiae s.l specimens assayed, 79 (92%) successfully amplified and all were identified as An. arabiensis. West African Kdr (L1014F) mutation was detected with high Kdr allele frequency ranging from 67–88%.ConclusionThe detection of target site mutation, kdr L1014F allele, coupled with the phenotypic resistance against alpha-cypermethrin and deltamethrin call for continuous resistance monitoring.
Nature Restoration Shifts the Abundance and Structure of Soil Nematode Communities in Subtropical China
Authors: Wang Jianqing , Zheng Yingfeng , Shi Xiuzhen , Lam Shu Kee , Lucas-Borja Manuel Esteban , Huang Zhiqun 
Published: July 2021

Abstract
Aims Soil nematode community is an important component of the soil food web, which has been widely recognized as a key bio-indicator for assessing the influence of nature restoration on ecological functions. However, the dynamics of the abundance, diversity and function of soil nematode community remain unclear under different forest succession phases. Methods The soil nematode community of natural secondary forests was investigated using a chronosequence approach. Nature restoration for five succession stages were sampled in this study to represent a wide range of stand age groups.Results Soil nematode abundance gradually increased with forest stand age, which reached a peak value (574 individuals 100 g-1 dry soil) in the older age classes. In contrast, soil nematode diversity was not affected by forest stand age. Soil available nitrogen and phosphorus were key factors influencing soil nematode abundance and diversity during forest secondary succession. The plant parasite index decreased with forest stand age, which indicated that ecosystem function and health would be improved as nature restoration progresses. In addition, the structure of soil nematode community was more sensitive to forest secondary succession compared to plant community and soil microbial community. The bottom-up effects of the plant and microbial communities on soil nematode community were important drivers of nematode community structure in subtropical forests. Conclusions Overall, this study demonstrates the active responses of soil nematode community to nature restoration, and highlights the importance of the above-ground and below-ground interactions to the soil food web.
Biocompatible Self-Healing Hydrogels Based On Boronic Acid-Functionalized Polymer And Laponite Nanocomposite Applied For Water Pollutant Removal
Authors: Wang Xue-Ting , Deng Xudong , Zhang Tuo-Di , Zhang Xi , Shi Wen-Pu , Lai Jialiang , Zhou Hongwei , Ye Ya-Jing , Zhang Chen-Yan , Yin Da-Chuan 
Published: July 2021

Abstract
The problem of global water pollution is becoming more and more severe, among which organic dyes and heavy metal ions are two typical types of the most common pollutants. The adsorption method for water purification and wastewater treatment is widely studied and applied. Hydrogel has unique advantages in the field of adsorption due to its three-dimensional porous structure. In this paper, a new type of self-healing hydrogels based on reversible covalent bond were prepared by mixing poly(vinyl alcohol) (PVA) and 2-aminophenylboronic acid modified polyacrylic acid (PAA-2APBA). In addition, the introduction of laponite nanoparticles into the hydrogel can increase both the mechanical strength and adsorption efficiency. This low-cost PAA-2APBA/PVA/laponite nano-composite hydrogel could efficiently remove the organic dyes and heavy metal ions in model waste water through simple immersion, which makes it have application prospects in the fields of both biomedical and environmental engineering.
Idiopathic Pericardial Effusion in Patients with Hypertrophic Cardiomyopathy
Authors: Puwanant Sarinya , Kittipibul Veraprapas , Songsirisuk Nattakorn , Santisukwongchote Sakun , Sitticharoenchai Patita , Chatranukulchai Pairoj , Sattithumanid Sudarat , Boonyaratvej Smonporn 
Published: July 2021

Abstract
Objectives: The aims of this study were to examine the prevalence of moderate to large (moderate-large) idiopathic pericardial effusion (i-PEF) in patients with hypertrophic cardiomyopathy (HCM) and to identify clinical and echocardiographic hemodynamic profiles associated with pericardial effusion. Methods: A total of 292 adult patients with HCM were studied. Fifteen patients with a history of factors associated with pericardial effusion including myocardial infarction, heart surgery or cardiac procedure within the last 12 months, autoimmune disease, hydralazine use, chronic kidney disease stage 3-4, tuberculosis, and malignancy were excluded. Results: Of 277 eligible patients with HCM, 11 patients (4%) with moderate-large i-PEF were identified. Clinical tamponade was present in 1 patient. Compared to patients with HCM who had no or small pericardial effusion, patients with moderate-large i-PEF were younger and more likely to have right ventricular (RV) hypertrophy and reverse septal curvature. These patients also exhibited a greater maximal septal thickness, mean and systolic pulmonary pressure, and right atrial pressure (p<0.05 for all). Pericardial fluid analysis and histopathological exams were performed in 7 and 3 patients, respectively. All examinations revealed transudative and nonspecific etiology of pericardial effusion. Conclusions: Idiopathic pericardial effusion and cardiac tamponade in patients with HCM was uncommon. The pathophysiology involved in pericardial effusion remains undetermined. Patients with moderate-large i-PEF frequently exhibited a phenotype of pulmonary hypertension and RV pressure overload.