bluuebunny/crossref_metadata_2025_split huggingface dataset:
Listeria monocytogenes requires cellular respiration for NAD+ regeneration and pathogenesis
Authors: Rivera-Lugo Rafael , Deng David , Anaya-Sanchez Andrea , Tejedor-Sanz Sara , Reyes Ruiz Valeria M , Smith Hans B , Titov Denis V , Sauer John-Demian , Skaar Eric P , Ajo-Franklin Caroline M , Portnoy Daniel A , Light Samuel H 
Published: November 2021

AbstractCellular respiration is essential for multiple bacterial pathogens and a validated antibiotic target. In addition to driving oxidative phosphorylation, bacterial respiration has a variety of ancillary functions that obscure its contribution to pathogenesis. We find here that the intracellular pathogen Listeria monocytogenes encodes two respiratory pathways which are partially functionally redundant and indispensable for pathogenesis. Loss of respiration decreased NAD+ regeneration, but this could be specifically reversed by heterologous expression of a water-forming NADH oxidase (NOX). NOX expression fully rescued intracellular growth defects and increased L. monocytogenes loads >1,000-fold in a mouse infection model. Consistent with NAD+ regeneration maintaining L. monocytogenes viability and enabling immune evasion, a respiration-deficient strain exhibited elevated bacteriolysis within the host cytosol and NOX rescued this phenotype. These studies show that NAD+ regeneration, rather than oxidative phosphorylation, represents the primary role of L. monocytogenes respiration and highlight the nuanced relationship between bacterial metabolism, physiology, and pathogenesis.
Single chain models illustrate the 3D RNA folding shape during translation
Authors: Guo Tianze , Modi Olivia L. , Hirano Jillian , Guzman Horacio V. , Tsuboi Tatsuhisa 
Published: November 2021

AbstractThe three-dimensional conformation of RNA is important in the function and fate of the molecule. The common conformation of mRNA is formed based on the closed-loop structure and internal base pairings with the activity of the ribosome movements. However, recent reports suggest that the closed-loop structure might not be formed in many mRNAs. This implies that mRNA can be considered as a single polymer in the cell. We developed TRIP; Three-dimensional RNA Illustration Program, to model the three-dimensional RNA folding shape based on single-chain models. We identified the angle restriction of each bead component from previously reported single-molecule FISH experimental data. This simulation method was able to recapitulate the mRNA conformation change of the translation activity and three-dimensional positional interaction between organelle and its localized mRNAs. Within the analyzed cases base-pairing interactions only have minor effects on the three-dimensional mRNA conformation, and instead single-chain polymer characteristics have a more significant impact on the conformation. This method will be used to predict the aggregation mechanism of mRNA and long noncoding RNA in specific cellular conditions such as nucleolus and phase-separated granules.
Directed evolution of colE1 plasmid replication compatibility: a fast tractable tunable model for investigating biological orthogonality
Authors: Chaillou Santiago , Stamou Eleftheria-Pinelopi , Torres Leticia , Riesco Ana B. , Hazelton Warren , Pinheiro Vitor B. 
Published: November 2021

AbstractPlasmids of the ColE1 family are among the most frequently used plasmids in molecular biology. They were adopted early in the field for many biotechnology applications, and as model systems to study plasmid biology. The mechanism of replication of ColE1 plasmids is well understood, involving the interaction between a plasmid-encoded sense-antisense gene pair (RNAI and RNAII). Because of its mechanism of replication, bacterial cells cannot maintain two different plasmids with the same origin, with one being rapidly lost from the population – a process known as plasmid incompatibility. While mutations in the regulatory genes RNAI and RNAII have been reported to make colE1 plasmids more compatible, there has been no attempt to engineer compatible colE1 origins, which can be used for multi-plasmid applications and that can bypass design constrains created by the current limited plasmid origin repertoire available. Here, we show that by targeting sequence diversity to the loop regions of RNAI (and RNAII), it is possible to select new viable colE1 origins that are compatible with the wild-type one. We demonstrate origin compatibility is not simply determined by sequence divergence in the loops, and that pairwise compatibility is not an accurate guide for higher order interactions. We identify potential principles to engineer plasmid copy number independently from other regulatory strategies and we propose plasmid compatibility as a tractable model to study biological orthogonality. New characterised plasmid origins increase flexibility and accessible complexity of design for challenging synthetic biology applications where biological circuits can be dispersed between multiple independent genetic elements.
The reproduction of Gram-negative protoplasts and the influence of environmental conditions on this process
Authors: Kanaparthi Dheeraj , Lampe Marko , Krohn Jan-Hagen , Zhu Baoli , Klingl Andreas , Lueders Tillmann 
Published: November 2021

SummaryBacterial protoplasts are known to reproduce independently of canonical molecular biological processes. Their reproduction is shown to be mediated entirely by the physicochemical properties of cell constituents. However, the physiochemical properties of the cell constituents are influenced by the environmental conditions like salinity, salt composition, and mechanical stresses experienced by a cell in natural environments. The influence of such environmental conditions on protoplast reproduction is seldom investigated. Here, we studied protoplast reproduction in their native environmental conditions. Contrary to the previous perceptions of protoplasts reproducing in an erratic manner, cells in our study reproduced in a defined sequence of steps. The process of their reproduction can be explained by an interplay between intracellular metabolism, the physicochemical properties of cell constituents, and the nature of cations in the growth media. We observed a minimal leakage of intracellular constituents during protoplast reproduction, suggesting an efficient reproduction. However, the efficiency of reproduction is determined by the environmental conditions. Under favorable environmental conditions, protoplasts reproduce with nearly similar efficiency to cells that possess a cell wall. In short, here we demonstrate the simplest method of cellular reproduction and the influence of environmental conditions on this process.
Impact of COVID-19 pandemic on financial reporting and disclosure practices: empirical evidence from Bangladesh
Authors: Sultana Reajmin , Ghosh Ratan , Sen Kanon Kumar 
Published: November 2021

PurposeTo investigate the consequence of COVID-19 pandemic on the financial reporting and disclosure (FRD) practices, the study has been conducted. Moreover, this paper highlights the significance of FRD practices in any emergency period and its relevance with legitimacy theory in Bangladesh Perspective.Design/methodology/approachThe COVID-19 pandemic has adverse impact on business. Hence, all the business activities have been categorized into five major aspects which are financial factors, business operations, business contracts, business value and stakeholders. These five major activities have been considered as independent variable. By analyzing various policy recommendations and guidelines of global and local accounting bodies, a structured questionnaire was developed in association with related IAS and IFRSs. Then, it was distributed among the accounting professionals of Bangladesh who are currently engaged in financial statement preparation and auditing services. Finally, data was analyzed through structural equation modeling (SEM) to test the hypothetical relationship between dependent variable and independent variable.FindingsThis study finds that financial factors, business contracts and stakeholders have significant relationship with the financial reporting and disclosure practices during the COVID-19 pandemic period. However, business operation and business value have no significant relationship with financial reporting and disclosure practices.Research limitations/implicationsThis study tries to analyze why and how firms should disclose essential information (both financial and non-financial) to the financial statement users during the COVID-19 pandemic. This study can be used as benchmark to issue a separate policy or standard for reporting any kind of adverse event in the financial reporting and disclosure practices.Originality/valueTo our best knowledge, we believe that this is first kind of study undertaken to investigate the consequence of COVID-19 pandemic on the FRD practices in the context of Bangladesh. This study is kind of exploratory in nature. Hence, future studies can explore industry-based financial reporting and disclosure practice in any pandemic period.
Lifestyle segmentation of older Koreans: a longitudinal comparison of segments and life satisfaction
Authors: Jung Kwon , Jung Jihye 
Published: November 2021

PurposeAging is a global phenomenon for many countries, and South Korea has become the fastest aging country in the world. The purpose of this study is to identify and validate a representative typology of older Koreans based on their lifestyle and value orientations. This study also makes a longitudinal comparison of these segments in two waves using similar survey data collected in 2009 and 2017 and examines any meaningful changes that occurred during the periods.Design/methodology/approachUsing survey data collected from 750 older adults living in four major cities in Korea, this study conducted factor and cluster analysis to identify lifestyle segmentation of elderly Koreans. Both descriptive and mean comparison analyses are followed to characterize the segments with relevant demographic and behavioral variables. In addition, this study makes a longitudinal comparison of these segments in two waves (2009 and 2017) and examines any meaningful changes that occurred during the periods.FindingsSix distinctive lifestyle segments of elderly Koreans are identified. The longitudinal comparison reveals some changes that occurred during the period. The level of importance of values and things to own has been declined during the period on most of the aspects, which can be interpreted that older Koreans become more realistic and practical. The level of life satisfaction between the two periods turned out to be similar. However, satisfaction in material comfort and health has been improved during the period, which means that the perception of their financial and physical aspects has been improved over the years.Originality/valueFirst, based on Tempest et al.’s three grey discontinuity framework (2002), the segmentation model provides comprehensive coverage of psychological transitions experienced by older people due to economic, physical and mental discontinuities with age. Second, this might be the first longitudinal study to examine the changes in segmentation over time in terms of the lifestyle of older adults. Practically, the findings of this study provide useful insights for policymakers in developing aging-related policies as well as for marketers who are interested or currently doing business in Korea or Asian consumer markets.
Numerical calculation of transient electric field considering space charge in oil-paper insulation of converter transformers
Authors: Xiu Liancheng , Du Zhiye , Tian Yu , He Jingxuan , Cai Hongwei , Yi Fan 
Published: November 2021

Purpose
The purpose of this paper is to develop a numerical simulation method based on the transient upstream finite element method (FEM) and Schottky emission theory to reveal the distribution characteristics of space charge in oil-paper insulation.

Design/methodology/approach
The main insulation medium of the converter transformer in high voltage direct current transmission is oil-paper insulation. However, the influence of space charge is difficult to be fully considered in the insulation design and simulation of converter transformers. To reveal the influence characteristics of the space charge, this paper proposes a numerical simulation method based on Schottky emission theory and the transient upstream FEM. This method considers the influence of factors, such as carrier mobility, carrier recombination coefficient, trap capture coefficient and diffusion coefficient on the basis of multi-physics field coupling calculation of the electric field and fluid field.

Findings
A numerical simulation method considering multiple charge states is proposed for the space charge problem in oil-paper insulation. Meanwhile, a space charge measurement platform based on the electrostatic capacitance probe method for oil-paper insulation structure is built, and the effectiveness and accuracy of the numerical simulation method is verified.

Originality/value
A variety of models are calculated and analyzed by the numerical simulation method in this paper, and the distribution characteristics of the space charge and total electric field in oil-paper insulation medium with single-layer, polarity reversal of plate voltage and double-layer are obtained. The research results of this paper have the guiding significance for the engineering application of oil-paper insulation and the optimal design of converter transformer insulation.
Brouges footwear: launching a Ghanian shoe brand
Authors: Vidal Jorge Fernandez 
Published: November 2021

Study level/applicability:
This case is intended for use in undergraduate, MBA and Executive Education courses in Strategy, Business in Africa, Entrepreneurship and Investing in Emerging Markets.

Subject area:
Strategy, Business in Africa, Entrepreneurship and Investing in Emerging Markets.

Case synopsis
Eric Kyere founded Brouges in 2015, an African brand, with the objective of designing, manufacturing and selling great shoes. His original plan was to produce Brouges’ shoes in Africa, but had several problems with suppliers (e.g. poor quality, poor raw materials, etc.). Therefore, Brouges had to partner with a European manufacturer to produce its first batch of shoes. Shortly thereafter, Eric partnered with Michael Asare Bediako, a young Ghanaian investor and entrepreneur, who had advanced plans to build a shoemaking factory in Ghana. The factory was likely to open in early 2021, following major delays caused by the COVID-19 pandemic. This would be a major milestone for Brouges and a key step in Eric’s entrepreneurial journey. However, how can Eric and Brouges manage the challenge of growing and building a manufacturing and retail organization? How could they succeed where others had failed?

Leaning objectives
This case has five pedagogical objectives: strategic choices: evaluate the strategic choices that companies need to make (based on their predefined goals and aspirations) and show that they are effectively an integrated cascade of choices that need to be consistent and coherent; strategic planning: apply and analyze the key steps and key decision to be made as part of a high-level strategic plan; value proposition: apply and analyze the concept and key components of a company’s value proposition, leveraging Tovstiga’s framework; doing business in Africa: evaluate the specific generic challenges of doing business in Africa, particularly in the manufacturing sector; generalizability of frameworks: this case shows that the same frameworks that are used to analyze large firms and mature markets can be applied to smaller firms in less developed markets.

Complexity academic level
This case is intended for use in undergraduate, MBA and Executive Education courses in Strategy, Business in Africa, Entrepreneurship and Investing in Emerging Markets.

Supplementary materials
Teaching notes are available for educators only.

Subject code
CSS: 3 Entrepreneurship.
Can Maruti Suzuki’s wagonr steer through the repositioning drive?
Authors: Nagar Komal 
Published: November 2021

Case overview
Maruti Suzuki India Limited (MSIL), a joint venture between Maruti Udyog Limited, India and Suzuki Motors, Japan, is considering repositioning its WagonR brand amidst issues of overall decline in sales in the automobile industry. With a market share of more than 53%, MSIL is the market leader in passenger vehicle segment in India, yet it is facing difficulties in driving up sales. The company’s portfolio comprises entry-hatch, mid-hatch, premium-hatch, sedan, SUV/MUV, crossover and van. The case dilemma involves the decision that MSIL’s management should take for the repositioning of WagonR, a compact hatchback, at a time when the automobile industry is showing no signs of recovery. Is it opportune to reposition WagonR, given the current situation of the passenger car market in India? If yes, what can MSIL learn from its past positioning efforts and how can it use insights about consumers’ current perceptions of WagonR’s brand image to arrive at a repositioning decision?

Leaning objectives
Using the case will help address the following objectives: to expose students to the challenges of repositioning an established brand; appreciate the need for and importance of repositioning established brands; evaluate existing positioning and market conditions for making a sound decision; and develop analytical skills that will prepare them to make decisions in real business scenarios.

Complexity academic level
The study is suitable for Masters level students in courses on Marketing Management, but it can also work well in elective courses such as brand management.

Supplementary materials
Teaching notes are available for educators only.

Subject code
CSS 8: Marketing.
Understanding the influence of technological capability and exogenous pressure on business model dynamics: insights from a longitudinal case study
Authors: Su Jingqin , Zhang Shuai , Ma Huanhuan 
Published: November 2021

PurposeThe purpose of the study is to explore how technological capability and exogenous pressure interactively influence business model (BM) dynamics over time in new technology-based ventures.Design/methodology/approachThe study adopts a longitudinal case study of the BM innovations of a Chinese financial technology venture. The structural approach and temporal bracket are used to analyze and theorize the data.FindingsThe findings indicate that distinct contextual changes impel a firm to refine or abandon existing BMs over time. In different stages, the antecedents interactively influence BM dynamics with three successive patterns, namely pressure dominance, parallel influence and hybrid influence. While both antecedents trigger changes during the initiation and implementation of new BMs, they also serve as the filter and the enabler, respectively, during the ideation and integration of BMs.Research limitations/implicationsThe study inductively develops three propositions regarding the relationship between BM dynamics and its antecedents, which is based on the data collected from one single firm. Future research should test the propositions in other domains and take more cross-level antecedents into consideration.Originality/valueThe study contributes to the nascent research stream of BM dynamics by offering in-depth insights into the interaction of internal and external antecedents and by linking the differentiated roles of antecedents to the BM innovation process. The research offers some practical implications for new technology-based ventures seeking to develop BMs in a fast-changing environment.
New forms of intellectual activity in globalized society
Authors: Kulikov Sergey B. 
Published: November 2021

Purpose
The purpose of this paper is the foresight of new forms of intellectual activity in society.

Design/methodology/approach
This research examines the ways of predicting the development of intellectual activity. To reveal the topic, the author uses semiotics and the method of building possible worlds. The author explores intellectual activity in terms of sign systems. From this angle, the logic of the narrative expresses the order of the organization of intellectual activity. This approach reveals the connections between images of possible worlds and decision-making methods.

Findings
The author conceptually outlines the forms of intellectual activity in a globalized society. A globalized society is a complex of political, economic, cultural and scientific ties that spread throughout the world. The foresight of new forms of intellectual activity allows conceptually sketching the practical use of proper decision-making methods. These methods involve the use of artificial machine intelligence, collective intelligence, etc. Forms of intellectual activity are related to the worldbuilding that cause the development of culture through the development of knowledge. The description of forms of intellectual activity shows a promising way of humanitarian research in a globalized society.

Research limitations/implications
The research implies technological metaphors related to the history of culture and the role of intellectual activity in it.

Practical implications
The author examines the practical possibilities of applying traditional humanities for the development of new forms of intellectual activity in a globalized society.

Social implications
In the social space, exposing the effectiveness of traditional humanities helps to assess the consequences of using intellectual activity in practice.

Originality/value
The originality of this research is associated with the identification of links between the conceptual provisions of semiotics and the method for building possible worlds.
Sustainable development: a developmental evaluation of logistics higher education in the Sultanate of Oman based on two innovation approaches (the triple helix of innovation and innovation competencies)
Authors: Nikolaou Panagiotis 
Published: November 2021

PurposeThis research seeks to identify evidence of innovation elements in the implementation of a sustainable development ecosystem in the HE environment. For the purposes of this investigation, the use of developmental evaluation has been deemed appropriate to fully explore the depths of the topic.Design/methodology/approachThe research follows a qualitative approach of inductive reasoning. For the purpose of this developmental evaluation, the collection of information from several stakeholders has been pursued in the study. Both a semi-structured interview and documents analysis were used.FindingsDifferent awareness levels among logistics faculty members, while the incentives given are minimal. Availability of grants and lack of tenure are some of the reasons raised. Industry is not cooperative in providing placements/internships restricting students of industry experience. Internationalisation is slow and international collaborations limited. Limited freedom in topical discussions and their implications to learning.Research limitations/implicationsThe research has considered possible limitations and used other methods for triangulation of the findings.Practical implicationsLow awareness on the implementation of pedagogical approaches for innovation. Not all faculty can be innovative (owing to current practice) neither they are incentivised to be so. Government spending is very low on R&D – 0.136 of the GDP in 2016. The industry is not ready for University-Business Collaborations, therefore achieving a low theory to practice ratio for students.Originality/valueIn the context in which the research has taken place (HE in Oman), there has been no evaluation (and more so developmental evaluation) previously implemented. Additionally, a longitudinal study, integrated as part of an ESD system targeted to innovation could increase the innovation capacity of the country on the international innovation index.