bluuebunny/crossref_metadata_2025_split huggingface dataset:
Prospective association of air purifier use during pregnancy with the neurodevelopment of toddlers in the Japan Environment and Children’s Study
Authors: Matsumura Kenta , Hamazaki Kei , Tsuchida Akiko , Inadera Hidekuni ,   The Japan Environment and Children’s Study (JECS) Group, Kamijima Michihiro , Yamazaki Shin , Ohya Yukihiro , Kishi Reiko , Yaegashi Nobuo , Hashimoto Koichi , Mori Chisato , Ito Shuichi , Yamagata Zentaro , Nakayama Takeo , Iso Hiroyasu , Shima Masayuki , Kurozawa Youichi , Suganuma Narufumi , Kusuhara Koichi , Katoh Takahiko 
Published: September 2021

AbstractWe examined the association between maternal air purifier use during pregnancy and neurodevelopmental delay in toddlers by analysing data from 82,457 mother-toddler pairs. Air purifier use was measured using a simple yes/no question. Developmental delays at 1.5, 2.0, 2.5, and 3.0 years were assessed using the Ages and Stages Questionnaire, Third Edition. Generalized additive mixed model analysis with 21 covariates revealed that air purifier use was associated with lower prevalence of developmental delay in all five areas—communication, gross motor, fine motor, problem solving, and personal-social—at all four time points (adjusted risk ratios ranged from 0.827 to 0.927, and only one 95% confidence interval crossed the reference). These findings suggest a negative association between air purifier use during pregnancy and neurodevelopmental delay in toddlers.Trial registration: UMIN000030786 (15/01/2018).
Electronic data collection to enhance disease surveillance at the slaughterhouse in a smallholder production system
Authors: Falzon Laura C. , Ogola Joseph G. , Odinga Christian O. , Naboyshchikov Leonid , Fèvre Eric M. , Berezowski John 
Published: September 2021

AbstractGlobally, meat inspection provides data for animal health surveillance. However, paper-based recording of data is often not reported through to higher authorities in sufficient detail. We trialled the use of an electronic meat inspection form in Kenyan slaughterhouses, in lieu of the currently used paper-based format. Meat inspectors in two ruminant slaughterhouses completed and submitted an electronic report for each animal slaughtered at their facility. The reports, which captured information on the animal demographics and any eventual condemnations, were stored in a central database and available in real-time. A stakeholder meeting was held towards the end of the study. Over the 2.75 year study period, 16,386 reports were submitted; a downward linear trend in the monthly submissions was noted. There was a week effect, whereby more reports were submitted on the market day. Of the slaughtered animals, 23% had at least a partial condemnation. The most frequently condemned organs were the liver, lungs and intestines; the primary reasons for condemnations were parasitic conditions. Lack of feedback and difficulty capturing animal origin information were the primary challenges highlighted. The study demonstrated that electronic data capture is feasible in such challenging environments, thereby improving the timeliness and resolution of the data collected.
UAV reveals substantial but heterogeneous effects of herbivores on Arctic vegetation
Authors: Siewert Matthias B. , Olofsson Johan 
Published: September 2021

AbstractUnderstanding how herbivores shape plant biomass and distribution is a core challenge in ecology. Yet, the lack of suitable remote sensing technology limits our knowledge of temporal and spatial impacts of mammal herbivores in the Earth system. The regular interannual density fluctuations of voles and lemmings are exceptional with their large reduction of plant biomass in Arctic landscapes during peak years (12–24%) as previously shown at large spatial scales using satellites. This provides evidence that herbivores are important drivers of observed global changes in vegetation productivity. Here, we use a novel approach with repeated unmanned aerial vehicle (UAV) flights, to map vegetation impact by rodents, indicating that many important aspects of vegetation dynamics otherwise hidden by the coarse resolution of satellite images, including plant–herbivore interactions, can be revealed using UAVs. We quantify areas impacted by rodents at four complex Arctic landscapes with very high spatial resolution UAV imagery to get a new perspective on how herbivores shape Arctic ecosystems. The area impacted by voles and lemmings is indeed substantial, larger at higher altitude tundra environments, varies between habitats depending on local snow cover and plant community composition, and is heterogeneous even within habitats at submeter scales. Coupling this with spectral reflectance of vegetation (NDVI), we can show that the impact on central ecosystem properties like GPP and biomass is stronger than currently accounted for in Arctic ecosystems. As an emerging technology, UAVs will allow us to better disentangle important information on how herbivores maintain spatial heterogeneity, function and diversity in natural ecosystems.
Multifaceted empathy differences in children and adults with autism
Authors: Quinde-Zlibut Jennifer M. , Williams Zachary J. , Gerdes Madison , Mash Lisa E. , Heflin Brynna H. , Cascio Carissa 
Published: September 2021

AbstractAlthough empathy impairments have been reported in autistic individuals, there is no clear consensus on how emotional valence influences this multidimensional process. In this study, we use the Multifaceted Empathy Test for juveniles (MET-J) to interrogate emotional and cognitive empathy in 184 participants (ages 8–59 years, 83 autistic) under the robust Bayesian inference framework. Group comparisons demonstrate previously unreported interaction effects between: (1) valence and autism diagnosis in predictions of emotional resonance, and (2) valence and age group in predictions of arousal to images portraying positive and negative facial expressions. These results extend previous studies using the MET by examining differential effects of emotional valence in a large sample of autistic children and adults with average or above-average intelligence. We report impaired cognitive empathy in autism, and subtle differences in emotional empathy characterized by less distinction between emotional resonance to positive vs. negative facial expressions in autism compared to neurotypicals. Reduced emotional differentiation between positive and negative affect in others could be a mechanism for diminished social reciprocity that poses a universal challenge for people with autism. These component- and valence- specific findings are of clinical relevance for the development and implementation of target-specific social interventions in autism.
Mitigating multiple stresses in Pangasianodon hypophthalmus with a novel dietary mixture of selenium nanoparticles and Omega-3-fatty acid
Authors: Kumar Neeraj , Singh Dilip Kumar , Bhushan Shashi , Jamwal Ankur 
Published: September 2021

AbstractEffects of a novel dietary mixture of selenium nanoparticles (Se-NPs) and omega-3-fatty acids i.e., Eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) on mitigating arsenic pollution, high-temperature stress and bacterial infection were investigated in Pangasianodon hypophthalmus. To aim this, four isocaloric and iso-nitrogenous diets were prepared: control feed (no supplementation), Se-NPs at 0.2 mg kg−1 diet with EPA + DHA at 0.2, 0.4 and 0.6% as supplemented diets. Fish were reared under normal condition or concurrent exposure to arsenic (2.65 mg L−1), and temperature (34 °C) (As + T) stress for 105 days. The experiment was conducted with eight treatments in triplicates. Response to various stresses i.e., primary (cortisol), secondary (oxidative stress, immunity, and stress biomarkers) and tertiary stress response (growth performance, bioaccumulation and mortality due to bacterial infection) were determined. Supplementation of dietary Se-NPs at 0.2 mg kg−1 diet and EPA + DHA at 0.2 and 0.4% reduced the primary stress level. Exposure to arsenic and temperature (As + T) and fed with control diet and EPA + DHA at 0.6% aggravated the cortisol level. Anti-oxidative enzymes (Catalase, superoxide dismutase, glutathione peroxidase and glutathione-s-transferase) and immunity (Nitroblue tetrazolium, total protein, albumin, globulin, A:G ratio, total immunoglobulin and myeloperoxidase) of the fish were augmented by supplementation of Se-NPs and EPA + DHA at 0.2 and 0.4%. Neurotransmitter enzyme, HSP 70, Vitamin C were significantly enhanced (p < 0.01) with supplementation of Se-NPs at 0.2 mg kg−1 and EPA + DHA at 0.2 and 0.4%. Whereas total lipid, cholesterol, phospholipid, triglyceride and very low-density lipoprotein (VLDL) were reduced (p < 0.01) with the supplementation of Se-NPs at 0.2 mg kg−1 diet and EPA + DHA at 0.2 and 0.4%. Tertiary stress response viz. growth performance was also significantly enhanced with supplementation of Se-NPs at 0.2 mg kg−1 and EPA + DHA at 0.2 and 0.4% reared under As + T. Whereas arsenic bioaccumulation in fish tissues was significantly reduced with dietary supplementation of Se-NPs and EPA + DHA. Cumulative mortality and relative percentage survival were reduced with Se-NPs at 0.2 mg kg−1 and EPA + DHA at 0.2 and 0.4%. The investigation revealed that a novel combination of Se-NPs at 0.2 mg kg−1 and EPA + DHA at 0.4% followed by 0.2% has the potential to alleviate temperature stress, bacterial infection and arsenic pollution. Whereas diet containing Se-NPs at 0.2 mg kg−1 diet and EPA + DHA at 0.6% was noticeably enhanced the stress in P. hypophthalmus.
Degree of anisogamy is unrelated to the intensity of sexual selection
Authors: Mokos Judit , Scheuring István , Liker András , Freckleton Robert P. , Székely Tamás 
Published: September 2021

AbstractMales and females often display different behaviours and, in the context of reproduction, these behaviours are labelled sex roles. The Darwin–Bateman paradigm argues that the root of these differences is anisogamy (i.e., differences in size and/or function of gametes between the sexes) that leads to biased sexual selection, and sex differences in parental care and body size. This evolutionary cascade, however, is contentious since some of the underpinning assumptions have been questioned. Here we investigate the relationships between anisogamy, sexual size dimorphism, sex difference in parental care and intensity of sexual selection using phylogenetic comparative analyses of 64 species from a wide range of animal taxa. The results question the first step of the Darwin–Bateman paradigm, as the extent of anisogamy does not appear to predict the intensity of sexual selection. The only significant predictor of sexual selection is the relative inputs of males and females into the care of offspring. We propose that ecological factors, life-history and demography have more substantial impacts on contemporary sex roles than the differences of gametic investments between the sexes.
GWAS identifies an ortholog of the rice D11 gene as a candidate gene for grain size in an international collection of hexaploid wheat
Authors: Tekeu Honoré , Ngonkeu Eddy L. M. , Bélanger Sébastien , Djocgoué Pierre F. , Abed Amina , Torkamaneh Davoud , Boyle Brian , Tsimi Patrick M. , Tadesse Wuletaw , Jean Martine , Belzile François 
Published: September 2021

AbstractGrain size is a key agronomic trait that contributes to grain yield in hexaploid wheat. Grain length and width were evaluated in an international collection of 157 wheat accessions. These accessions were genetically characterized using a genotyping-by-sequencing (GBS) protocol that produced 73,784 single nucleotide polymorphism (SNP) markers. GBS-derived genotype calls obtained on Chinese Spring proved extremely accurate when compared to the reference (> 99.9%) and showed > 95% agreement with calls made at SNP loci shared with the 90 K SNP array on a subset of 71 Canadian wheat accessions for which both types of data were available. This indicates that GBS can yield a large amount of highly accurate SNP data in hexaploid wheat. The genetic diversity analysis performed using this set of SNP markers revealed the presence of six distinct groups within this collection. A GWAS was conducted to uncover genomic regions controlling variation for grain length and width. In total, seven SNPs were found to be associated with one or both traits, identifying three quantitative trait loci (QTLs) located on chromosomes 1D, 2D and 4A. In the vicinity of the peak SNP on chromosome 2D, we found a promising candidate gene (TraesCS2D01G331100), whose rice ortholog (D11) had previously been reported to be involved in the regulation of grain size. These markers will be useful in breeding for enhanced wheat productivity.
Mathematical modeling based on RT-qPCR analysis of SARS-CoV-2 in wastewater as a tool for epidemiology
Authors: Krivoňáková Naďa , Šoltýsová Andrea , Tamáš Michal , Takáč Zdenko , Krahulec Ján , Ficek Andrej , Gál Miroslav , Gall Marián , Fehér Miroslav , Krivjanská Anna , Horáková Ivana , Belišová Noemi , Bímová Paula , Škulcová Andrea Butor , Mackuľak Tomáš 
Published: September 2021

AbstractCoronavirus disease 2019 (COVID-19) pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) emerges to scientific research and monitoring of wastewaters to predict the spread of the virus in the community. Our study investigated the COVID-19 disease in Bratislava, based on wastewater monitoring from September 2020 until March 2021. Samples were analyzed from two wastewater treatment plants of the city with reaching 0.6 million monitored inhabitants. Obtained results from the wastewater analysis suggest significant statistical dependence. High correlations between the number of viral particles in wastewater and the number of reported positive nasopharyngeal RT-qPCR tests of infected individuals with a time lag of 2 weeks/12 days (R2 = 83.78%/R2 = 52.65%) as well as with a reported number of death cases with a time lag of 4 weeks/27 days (R2 = 83.21%/R2 = 61.89%) was observed. The obtained results and subsequent mathematical modeling will serve in the future as an early warning system for the occurrence of a local site of infection and, at the same time, predict the load on the health system up to two weeks in advance.
Novel technology at hand to measure skin hydration by Biodisplay smartphone touch screen panel
Authors: Choi YoungHwan , Oh Se Jin , Lee Jong Hee 
Published: September 2021

AbstractSkin hydration is generally evaluated using devices that measure capacitance or conductance. A new technology (Biodisplay) was developed to provide accurate measurements of skin hydration at the contact site. This study aimed to test the reliability of the Biodisplay by comparing its performance results with those of similar devices currently used to objectively assess skin hydration. For each of the 30 participants, skin hydration was measured at each of the defined points on the forearm three times using the Biodisplay and a Hydration probe (HP), an objective measurement device of skin hydration. We also evaluated skin hydration of the arm using both tools after applying moisturizers to evaluate interferences from skin care products. The reliability and reproducibility of each device were analyzed by intraclass correlation coefficients (ICC), and the correlation of the two devices was evaluated by Pearson’s correlation coefficients (PCC). The Biodisplay demonstrated moderate-to-excellent reliability (ICC: (0.741–0.980)), but lower reliability than the HP (ICC: (0.953–0.980)). The skin hydration measurements made by the two devices were demonstrated to be significantly correlated, showing moderate correlations (PCC: 0.601). The Biodisplay can provide reasonably reliable and accurate measurements for skin hydration with the strong points of portability and accessibility.
Modified hydrologic regime of upper Ganga basin induced by natural and anthropogenic stressors
Authors: Swarnkar Somil , Mujumdar Pradeep , Sinha Rajiv 
Published: September 2021

AbstractClimate change and anthropogenic activities pose serious threats to river basin hydrology worldwide. The Ganga basin is home to around half a billion people and has been significantly impacted by hydrological alterations in the last few decades. The increasing high-intensity rainfall events often create flash flooding events. Such events are frequently reported in mountainous and alluvial plains of the Ganga basin, putting the entire basin under severe flood risk. Further, increasing human interventions through hydraulic structures in the upstream reaches significantly alter the flows during the pre-and post-monsoon periods. Here, we explore the hydrological implications of increasing reservoir-induced and climate-related stressors in the Upper Ganga Basin (UGB), India. Flow/sediment duration curves and flood frequency analysis have been used to assess pre-and post-1995 hydrological behaviour. Our results indicate that low and moderate flows have been significantly altered, and the flood peaks have been attenuated by the operation of hydraulic structures in the Bhagirathi (western subbasin). The Alaknanda (eastern subbasin) has experienced an increase in extreme rainfall and flows post-1995. The downstream reaches experience reservoir-induced moderate flow alterations during pre-and post-monsoon and increasing extreme flood magnitudes during monsoon. Furthermore, substantial siltation upstream of the reservoirs has disrupted the upstream–downstream geomorphologic linkages.
Quantum halo states in two-dimensional dipolar clusters
Authors: Guijarro G. , Astrakharchik G. E. , Boronat J. 
Published: September 2021

AbstractA halo is an intrinsically quantum object defined as a bound state of a spatial size which extends deeply into the classically forbidden region. Previously, halos have been observed in bound states of two and less frequently of three atoms. Here, we propose a realization of halo states containing as many as six atoms. We report the binding energies, pair correlation functions, spatial distributions, and sizes of few-body clusters composed by bosonic dipolar atoms in a bilayer geometry. We find two very distinct halo structures, for large interlayer separation the halo structure is roughly symmetric and we discover an unusual highly anisotropic shape of halo states close to the unbinding threshold. Our results open avenues of using ultracold gases for the experimental realization of halos composed by atoms with dipolar interactions and containing as many as six atoms.
Production of IgG1-based bispecific antibody without extra cysteine residue via intein-mediated protein trans-splicing
Authors: Akiba Hiroki , Ise Tomoko , Nagata Satoshi , Kamada Haruhiko , Ohno Hiroaki , Tsumoto Kouhei 
Published: September 2021

AbstractA major class of bispecific antibodies (BsAbs) utilizes heterodimeric Fc to produce the native immunoglobulin G (IgG) structure. Because appropriate pairing of heavy and light chains is required, the design of BsAbs produced through recombination or reassembly of two separately-expressed antigen-binding fragments is advantageous. One such method uses intein-mediated protein trans-splicing (IMPTS) to produce an IgG1-based structure. An extra Cys residue is incorporated as a consensus sequence for IMPTS in successful examples, but this may lead to potential destabilization or disturbance of the assay system. In this study, we designed a BsAb linked by IMPTS, without the extra Cys residue. A BsAb binding to both TNFR2 and CD30 was successfully produced. Cleaved side product formation was inevitable, but it was minimized under the optimized conditions. The fine-tuned design is suitable for the production of IgG-like BsAb with high symmetry between the two antigen-binding fragments that is advantageous for screening BsAbs.
Defined d-hexapeptides bind CUG repeats and rescue phenotypes of myotonic dystrophy myotubes in a Drosophila model of the disease
Authors: Rapisarda Anna , Bargiela Ariadna , Llamusi Beatriz , Pont Isabel , Estrada-Tejedor Roger , Garcia-España Enrique , Artero Ruben , Perez-Alonso Manuel 
Published: September 2021

AbstractIn Myotonic Dystrophy type 1 (DM1), a non-coding CTG repeats rare expansion disease; toxic double-stranded RNA hairpins sequester the RNA-binding proteins Muscleblind-like 1 and 2 (MBNL1 and 2) and trigger other DM1-related pathogenesis pathway defects. In this paper, we characterize four d-amino acid hexapeptides identified together with abp1, a peptide previously shown to stabilize CUG RNA in its single-stranded conformation. With the generalized sequence cpy(a/t)(q/w)e, these related peptides improved three MBNL-regulated exon inclusions in DM1-derived cells. Subsequent experiments showed that these compounds generally increased the relative expression of MBNL1 and its nuclear-cytoplasmic distribution, reduced hyperactivated autophagy, and increased the percentage of differentiated (Desmin-positive) cells in vitro. All peptides rescued atrophy of indirect flight muscles in a Drosophila model of the disease, and partially rescued muscle function according to climbing and flight tests. Investigation of their mechanism of action supports that all four compounds can bind to CUG repeats with slightly different association constant, but binding did not strongly influence the secondary structure of the toxic RNA in contrast to abp1. Finally, molecular modeling suggests a detailed view of the interactions of peptide-CUG RNA complexes useful in the chemical optimization of compounds.
Relative sit-to-stand power cut-off points and their association with negatives outcomes in older adults
Authors: Baltasar-Fernandez Ivan , Alcazar Julian , Mañas Asier , Alegre Luis M. , Alfaro-Acha Ana , Rodriguez-Mañas Leocadio , Ara Ignacio , García-García Francisco J. , Losa-Reyna Jose 
Published: September 2021

AbstractThe purposes of this study were: (i) to evaluate the association of sit-to-stand (STS) power and body composition parameters [body mass index (BMI) and legs skeletal muscle index (SMI)] with age; (ii) to provide cut-off points for low relative STS power (STSrel), (iii) to provide normative data for well-functioning older adults and (iv) to assess the association of low STSrel with negative outcomes. Cross-sectional design (1369 older adults). STS power parameters assessed by validated equations, BMI and Legs SMI assessed by dual-energy X-ray absorptiometry were recorded. Sex- and age-adjusted segmented and logistic regression analyses and receiver operator characteristic curves were used. Among men, STSrel showed a negative association with age up to the age of 85 years (− 1.2 to − 1.4%year−1; p < 0.05). In women, a negative association with age was observed throughout the old adult life (− 1.2 to − 2.0%year−1; p < 0.001). Cut-off values for low STSrel were 2.5 W kg−1 in men and 1.9 W kg−1 in women. Low STSrel was associated with frailty (OR [95% CI] = 5.6 [3.1, 10.1]) and low habitual gait speed (HGS) (OR [95% CI] = 2.7 [1.8, 3.9]) in men while low STSrel was associated with frailty (OR [95% CI] = 6.9 [4.5, 10.5]) low HGS (OR [95% CI] = 2.9 [2.0, 4.1]), disability in activities of daily living (OR [95% CI] = 2.1 [1.4, 3.2]), and low quality of life (OR [95%CI] = 1.7 [1.2, 2.4]) in women. STSrel declined with increasing age in both men and women. Due to the adverse outcomes related to STSrel, the reported cut-off points can be used as a clinical tool to identify low STSrel among older adults.