bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cloning, Prokaryotic Expression, and Purification of Acetyl-CoA C-Acetyltransferase
from Atractylodes lancea
Authors: Wu Junxian , Liu Weiwei , Lu Jimei , Xu Rui , Xie Jin , Zha Liangping 
Published: November 2021

Background:
Cangzhu (Atractylodes lancea), a valuable and common traditional Chinese
medicinal herb, is primarily used as an effective medicine with various health-promoting effects.
The main pharmacological bioactive ingredients in the rhizome of A. lancea are terpenoids.
Acetyl-CoA C-acetyltransferase (AACT) is the first enzyme in the terpenoid synthesis pathway
and catalyzes two units of acetyl-CoA into acetoacetyl-CoA.

Objective:
The objective of the present work was to clone and identify function of AlAACT from
Atractylodes lancea.

Method:
A full-length cDNA clone of AlAACT was isolated using PCR and expressed in
Escherichia coli. The expressed protein was purified using Ni-NTA agarose column using standard
protocols. AlAACT was transiently expressed in N. benthamiana leaves to determine their subcellular
location. The difference in growth between recombinant bacteria and control bacteria under different
stresses was observed using the droplet plate experiment.

Result:
In this study, a full-length cDNA of AACT (AlAACT) was cloned from A. lancea, which
contains a 1,227 bp open reading frame and encodes a protein with 409 amino acids. Bioinformatic
and phylogenetic analysis clearly suggested that AlAACT shared high similarity with AACTs from
other plants. The recombinant protein pET32a(+)/AlAACT was successfully expressed in
Escherichia coli BL21 (DE3) cells induced with 0.4 mM IPTG at 30°C as the optimized condition.
The recombinant enzyme pET-32a-AlAACT was purified using the Ni-NTA column based on the
His-tag, and the molecular weight was determined to be 62 kDa through SDS-PAGE and Western
Blot analysis. The recombinant protein was eluted with 100, 300, and 500 mM imidazole; most of
the protein was eluted with 300 mM imidazole. Under mannitol stress, the recombinant pET-32a-
AlAACT protein showed a substantial advantage in terms of growth rates compared to the control.
However, this phenomenon was directly opposite under NaCl abiotic stress. Subcellular localization
showed that AlAACT localizes to the nucleus and cytoplasm.

Conclusion:
The expression and purification of recombinant enzyme pET-32a-AlAACT were successful,
and the recombinant strain pET-32a-AlAACT in showed better growth in a drought stress.
The expression of AlAACT-EGFP fusion protein revealed its localization in both nuclear and cytoplasm
compartments. This study provides an important foundation for further research into the effects
of terpenoid biosynthesis in A. lancea.
Update on the Biological Relevance of Lysine Acetylation as a Novel
Drug Target in Trypanosomatids
Authors: Peralta Gonzalo Martinez , Serra Esteban , Alonso Victoria Lucia 
Published: November 2021

:
The number of acetylated proteins identified from bacteria to mammals has
grown exponentially in the last ten years, and it is now accepted that acetylation is a key
component in most eukaryotic signaling pathways and is as important as phosphorylation.
The enzymes involved in this process are well described in mammals; acetyltransferases
and deacetylases are found inside and outside the nuclear compartment and have
different regulatory functions. In trypanosomatids, several of these enzymes have been
described and are postulated to be novel antiparasitic targets for the rational design of
drugs. In this review article, we present an update of the most important known acetylated
proteins in trypanosomatids, analyzing the acetylomes available. Also, we summarize
the information available regarding acetyltransferases and deacetylases in trypanosomes
and their potential use as chemotherapeutic targets.
Study on the Relationship between Rivaroxaban and Factor Xa Activity in Blood
Based on HPLC-MS/MS
Authors: Liu Hongchuan , Zhao Zhixia , Wang Yingkai , Liu Lihong , Yang Yuanhua , An Zhuoling 
Published: November 2021

Objective:
The aim of the study was to investigate a high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method for the determination of rivaroxaban and evaluate the correlation between plasma concentration and anti-Xa activity in patients using oral rivaroxaban.

Methods:
In this study, the plasma concentration of rivaroxaban and anti-Xa factor activities was determined in 125 patients, and the relationship between the two variables was analysed by SPSS 21.0 software.

Results:
The results showed that the plasma concentrations of oral rivaroxaban patients were significantly correlated with the activity of the anti-Xa factor (Spearman’s r = 0.990, p < 0.05).

Conclusion:
The plasma concentrations of rivaroxaban are a potentially useful monitoring indicator to assess the patient’s bleeding risk if testing for plasma anti-Xa activity is not available.
Recent Advances in Biotransformation by Cunninghamella Species
Authors: Gonçalves Manoela Daiele , Tomiotto-Pellissier Fernanda , de Matos Ricardo Luís Nascimento , Assolini João Paulo , da Silva Bortoleti Bruna Taciane , Concato Virginia Marcia , Silva Taylon Felipe , Rafael Janice Aparecida , Pavanelli Wander Rogério , Conchon-Costa Ivete , Arakawa Nilton Syogo 
Published: November 2021

:
The goal of the biotransformation process is to develop structural changes and generate new chemical
compounds, which can occur naturally in mammalian and microbial organisms, such as filamentous fungi, and represent
a tool to achieve enhanced bioactive compounds. Cunninghamella spp is among the fungal models most widely
used in biotransformation processes at phase I and II reactions, mimicking the metabolism of drugs and xenobiotics
in mammals and generating new molecules based on substances of natural and synthetic origin. Therefore, the goal
of this review is to highlight the studies involving the biotransformation of Cunninghamella species between January
2015 and March 2021, in addition to updating existing studies to identify the similarities between the human metabolite
and Cunninghamella patterns of active compounds, with related advantages and challenges, and providing new
tools for further studies in this scope.
Regulatory Effects of N-3 PUFAs on Pancreatic β-cells and Insulin-sensitive Tissues
Authors: Liu Wen , Zheng Qing , Zhu Min , Liu Xiaohong , Liu Jingping , Lu Yanrong , Cheng Jingqiu , Chen Younan 
Published: November 2021

:
The N-3 polyunsaturated fatty acids (PUFAs) have a wide range of health benefits, including antiinflammatory
effects, improvements in lipids metabolism and promoting insulin secretion, as well as reduction of
cancer risk. Numerous studies support that N-3 PUFAs have the potentials to improve many metabolic diseases, such
as diabetes, nonalcoholic fatty liver disease and obesity, which are attributable to N-3 PUFAs mediated enhancement
of insulin secretion by pancreatic β-cells and improvements in insulin sensitivity and metabolic disorders in peripheral
insulin-sensitive tissues such as liver, muscles, and adipose tissue. In this review, we summarized the up-to-date
clinical and basic studies on the regulatory effects and molecular mechanisms of N-3 PUFAs mediated benefits on
pancreatic β-cells, adipose tissue, liver, and muscles in the context of glucose and/or lipid metabolic disorders. We
also discussed the potential factors involved in the inconsistent results from different clinical researches of N-3 PUFAs.
Roles of M6A Regulators in Hepatocellular Carcinoma: Promotion or Suppression
Authors: Tang Hua , Chen Jiamao , Zhang Qian , Liu Ting 
Published: November 2021

Abstract:
Hepatocellular carcinoma (HCC) is the sixth globally diagnosed cancer with a poor prognosis.
Although the pathological factors of hepatocellular carcinoma are well elucidated, the underlying
molecular mechanisms remain unclear. N6-methyladenosine (M6A) is adenosine methylation
occurring at the N6 site, which is the most prevalent modification of eukaryotic mRNA. Recent
studies have shown that M6A can regulate gene expression, thus modulating the processes of cell
self-renewal, differentiation, and apoptosis. The methyls in M6A are installed by methyltransferases
(“writers”), removed by demethylases (“erasers”) and recognized by M6A-binding proteins
(“readers”). In this review, we discuss the roles of the above regulators in the progression and prognosis
of HCC, and summarize the clinical association between M6A modification and hepatocellular
carcinoma, so as to provide more valuable information for clinical treatment.
Pharmacological Medical Treatment of Epilepsy in Patients with Dementia: A Systematic Review
Authors: Musaeus Christian S. , Nilsson Christer , Cooper Chris , Kramberger Milica G. , Verdelho Ana , Stefanova Elka , Religa Dorota , Waldemar Gunhild , Frederiksen Kristian S. 
Published: November 2021

Background:
Patients with dementia have an increased risk of developing epilepsy, especially
in patients with vascular dementia and Alzheimer’s disease. In selecting the optimal anti-
epileptic drug (AED), the possible side effects such as drowsiness and worsening of cognitive
function should be taken into consideration, together with co-morbidities and type of epilepsy.

Objective:
The current systematic review investigates the efficacy, tolerability, and changes in cognitive
function after administration of AED in patients with dementia and epilepsy.

Methods:
We searched six databases, including MEDLINE and CENTRAL, checked reference
lists, contacted experts, and searched Google Scholar to identify studies reporting randomized
trials. Studies identified were independently screened, data extracted, and quality appraised by two
researchers. A narrative synthesis was used to report findings.

Results:
We included one study with 95 patients with Alzheimer’s disease randomized to either levetiracetam,
lamotrigine, or phenobarbital. No significant differences were found for efficacy, but
patients receiving levetiracetam showed an improvement in mini-mental state examination scores
and had fewer adverse events.

Conclusion:
High-quality evidence in the form of randomized controlled trials to guide clinicians
in choosing an AED in patients with dementia and concomitant epilepsy remains scarce. However,
levetiracetam has previously been shown to possibly improve cognition in patients with both mild
cognitive impairment and Alzheimer’s disease, is better tolerated in the elderly population, and has
no clinically relevant interaction with either cholinesterase inhibitors or NMDA receptor antagonists.
MR Brain Screening using Optimization Techniques - A Survey
Authors: Chitradevi D. , Prabha S. 
Published: November 2021

Background:
Alzheimer’s disease (AD) is associated with Dementia, and it is also a memory
syndrome in the brain. It affects the brain tissues and causes major changes in day-to-day activities.
Aging is a major cause of Alzheimer’s disease. AD is characterized by two pathological
hallmarks, Amyloid β protein and neurofibrillary tangles of hyperphosphorylated tau protein. The
imaging hallmarks for Alzheimer’s disease are swelling, shrinkage of brain tissues due to cell loss,
and atrophy in the brain due to protein dissemination. Based on the survey, 60% to 80% of dementia
patients belong to Alzheimer’s disease.

Introduction:
AD is now becoming an important brain disease. The goal of AD pathology is to
cause changes/damage in brain tissues. Alzheimer’s disease is thought to begin 20 years or more before
symptoms appear, with tiny changes in the brain that are undetectable to the person affected.
The changes in a person’s brain after a few years are noticeable through symptoms such as language
difficulties and memory loss. Neurons in different parts of the brain have detected symptoms
such as cognitive impairments and learning disabilities. In this case, neuroimaging tools are necessary
to identify the development of pathology which relates to the clinical symptoms.

Methods:
Several approaches have been tried during the last two decades for brain screening to
analyse AD using pre-processing, segmentation, and classification. Different individuals, such as
Grey Wolf optimization, Lion Optimization, Ant Lion Optimization, etc., have been attempted in
the proposed study. Similarly, hybrid optimization techniques are also attempted to segment the
brain sub-regions, which helps in identifying the biomarkers to analyse AD.

Conclusion:
This study discusses a review of neuroimaging technologies for diagnosing
Alzheimer’s disease, as well as the discovery of hallmarks for the disease and the methodologies
for finding hallmarks from brain images to evaluate AD. According to the literature review, most
of the techniques predicted higher accuracy (more than 90%), which is beneficial for assessing and
screening neurodegenerative disease, particularly Alzheimer’s disease.
Impact on Growth and Secondary Metabolites in White Poplar (Populus
alba L.) Callus using SeNPs@Moringa
Authors: Abo El-Fadl Reda E. , El-Saber Mahmoud M. , Ahmed Manal E.A. , Abd Elaziem Tamer M. , El-Sayed Ahmed A. 
Published: November 2021

Background:
Green synthesis of nanoparticles has provided a cost-effective, environmentally
friendly method, as well as raised safe strategies for the synthesis of nanomaterials.

Methods:
In this study, we investigated the synthesis of Selenium nanoparticles from the leaf aqueous
extract of Moringa oleifera (MO-SeNPs) and studied the effect of MO-SeNPs and Moringa extract
with various concentrations on the growth, active constituents, and antioxidant activity (scavenging
DPPH) of Populus alba callus. MS medium supplemented with (2, 4-D at 2 mg/l + 0.5 mg/l
BA) took the maximum callus induction percentage (100%), increasing the callus fresh weight, dry
weight, and degree of callus formation compared with (2.0 mg/l 2,4-D + 0.5 mg/l Kin).

Results:
The highest values of callus fresh and dry weight were recorded on MS containing 20%
MO-SeNPs. Regarding the active constituents, the GC-MS chromatogram revealed the presence of
23 major compounds identified in the chloroform extract. The phytoconstituents with maximum
peak area in callus extract are Tetradecane, Hexadecen-1-ol, Trans-9, 5-Octadecene, (Benzene, 1-
pentyloctyl), (Benzene, 1-butylnonyl), Cycloeicosane, and 10-Heneicosene in all treatments.
Moringa extract and MO-SeNPs stimulate the increase of compounds in callus compared to control.
In this regard, MO-SeNPs 5% led to a higher increase in the area percent of active substances compared
to the treatment of Moringa extract and control. Concerning the antioxidant activity, the results
indicated that Moringa extract and MO-SeNPs had a positive effect on scavenging DPPH
compared with the control. The highest percentages of scavenging DPPH were recorded when Populus
alba L. callus treated with MO-SeNPs 20%, 10%, and Moringa extract 20%, percentages
reached (84.65, 75.35, and 71.94%), respectively.

Conclusion :
Green synthesis of MO-SeNPs improved secondary metabolites and antioxidant activity
in Populus alba L. callus.
Smart Assistive Technology for Cooking for People With Cognitive Impairments Following a Traumatic Brain Injury: User Experience Study
Authors: Gagnon-Roy Mireille , Pinard Stéphanie , Bottari Carolina , Le Morellec Fanny , Laliberté Catherine , Ben Lagha Rym , Yaddaden Amel , Pigot Hélène , Giroux Sylvain , Bier Nathalie 
Published: November 2021

Background
User experience (UX), including usability, should be formally assessed multiple times throughout the development process to optimize the acceptability and integration of a new technology before implementing it within the home environment of people living with cognitive impairments.

Objective
The aim of this study is to identify UX issues, notably usability issues, and factors to consider for the future implementation of the COOK (Cognitive Orthosis for Cooking) within the home of individuals with traumatic brain injury (TBI) to identify modifications to improve the technology.

Methods
This study comprised two rounds of UX evaluations, including extensive usability testing, which were completed in a laboratory context: 3 sessions with 5 experts and, after improvement of COOK, 2 sessions with 10 participants with TBI. Each session included the use of scenarios and questionnaires on UX and usability.

Results
Both rounds demonstrated good usability outcomes and hedonic qualities. Various usability issues were identified by participants, such as navigation inconsistencies, technical bugs, and the need for more feedback. Factors to consider in the future implementation of COOK were also mentioned by participants with TBI, including environmental (eg, space available and presence of pets) and personal factors (eg, level of comfort with technology, presence of visual deficits, and preferences).

Conclusions
By evaluating UX, including usability, various times throughout the development process and including experts and end users, our research team was able to develop a technology that was perceived as usable, pleasant, and well-designed. This research is an example of how and when people with cognitive impairments (ie, people with TBI) can be involved in evaluating the UX of new technology.
Mechanisms of Change in Digital Health Interventions for Mental Disorders in Youth: Systematic Review
Authors: Domhardt Matthias , Engler Sophie , Nowak Hannah , Lutsch Arne , Baumel Amit , Baumeister Harald 
Published: November 2021

Background
Digital health interventions (DHIs) are efficacious for several mental disorders in youth; however, integrated, evidence-based knowledge about the mechanisms of change in these interventions is lacking.

Objective
This systematic review aims to comprehensively evaluate studies on mediators and mechanisms of change in different DHIs for common mental disorders in children and adolescents.

Methods
A systematic literature search of the electronic databases Cochrane Central Register of Controlled Trials, Embase, MEDLINE, and PsycINFO was conducted, complemented by backward and forward searches. Two independent reviewers selected studies for inclusion, extracted the data, and rated the methodological quality of eligible studies (ie, risk of bias and 8 quality criteria for process research).

Results
A total of 25 studies that have evaluated 39 potential mediators were included in this review. Cognitive mediators were the largest group of examined intervening variables, followed by a broad range of emotional and affective, interpersonal, parenting behavior, and other mediators. The mediator categories with the highest percentages of significant intervening variables were the groups of affective mediators (4/4, 100%) and combined cognitive mediators (13/19, 68%). Although more than three-quarters of the eligible studies met 5 or more quality criteria, causal conclusions have been widely precluded.

Conclusions
The findings of this review might guide the empirically informed advancement of DHIs, contributing to improved intervention outcomes, and the discussion of methodological recommendations for process research might facilitate mediation studies with more pertinent designs, allowing for conclusions with higher causal certainty in the future.
Cosmologías nahua (maseual) y totonaca (tutunakú) de la Sierra Norte de Puebla (México) Segunda parte: las ‘almas’, los dueños, las fuerzas maléficas
Authors: Beaucage Pierre , Rojas Mora Xanath 
Published: November 2021

<p>Continuando nuestra comparación entre las cosmologías indígenas de la Sierra Norte de Puebla (ver Beaucage y Rojas Mora 2021), e-<br /> xaminamos aquí, a partir de relatos y testimonios, los encuentros entre los humanos y los entes sobrenaturales que quedaron en el mundo una vez terminado el tiempo de la creación: Dueños de la Tierra, del Agua, del Fuego, y también seres malignos como los Aires y los tepeuanij. Esos encuentros a menudo tienen consecuencias sobre la salud, el ‘susto’ siendo la más común, pero no la única. Para entender esa patología espiritual y los tratamientos realizados por curanderos y chamanes, empezamos por aclarar la concepción de los ‘centros y entidades anímicas’ en las culturas nahua y totonaca. Distinguimos a tres: el ‘espíritu/corazón/alma’, la ‘sombra’ y el ‘doble animal’. Las fuerzas cósmicas afectan estas entidades de forma distinta y los tratamientos varían en consecuencia. Nuestro estudio pone a la luz diferencias<br /> importantes en conceptos y prácticas entre nahuas y totonacos, pero también variaciones entre comunidades de una etnia determinada. Se necesitan más estudios de caso para poder estimar bien la amplitud de estas diferencias.</p><div> </div>
«Slike mødre»: Barnevernets stigmatisering av sårbare familier i dagens Russland
Authors: Ofitserova Trine Skogset 
Published: November 2021

Abstract in English“That kind of mother”: Stigmatisation by the Russian child protection servicesHow does stigma influence the extent to which the child protection services in Russia undertake preventative work with vulnerable families in order to avoid taking the child out the family? Based on an analysis of previous research and my own fieldwork from St. Petersburg and Moscow, I note the gap between expectations towards vulnerable families, and their actual living conditions and potential for meeting these expectations. This gap results in a stigmatising categorisation of families who find themselves marginalised as neblagopolutsjnye – socially disadvantaged. Although Russia’s officially ‘family-oriented’ child protection system is expected to base its work on objective methods, stigmatisation influences the decisions of social workers, making the system risk-oriented in practice. This is due to little or inadequate specialised education among social workers, resulting in the strong presence of the human factor: subjective, non-professional evaluations are made. With scant intersectional cooperation among state actors, there is little space to counteract or modify subjective evaluations. Thus, stigmatisation serves to limit the extent to which vulnerable families in Russia receive help, thus creating and upholding ‘social orphanhood’.
Pengaruh Media Kartu Kuartet Terhadap Penguasaan Hiragana Siswa Kelas X SMAN 6 Padang
Authors: Marta Afriyona , Yulia Nova 
Published: November 2021

AbstrakProses pembelajaran Bahasa Jepang di Indonesia masih sering menemui beberapa kendala dalam mempelajari huruf. Penggunaan huruf yang berbeda menjadi salah satu faktor yang menyebabkan pembelajar merasa kesulitan dalam mempelajari Bahasa Jepang. Karena ada prinsip yang tidak terpenuhi yaitu penggunaan media serta metode atau teknik yang digunakan tidak sesuai. Berdasarkan apa yang peneliti amati pada saat melaksanakan praktik lapangan kependidikan (PLK) di SMAN 6 Padang serta wawancara yang peneliti lakukan dengan siswa, menunjukkan minimnya minat dan kemampuan siswa dalam mempelajari hiragana. Sehingga pencapaian tujuan belajar tidak maksimal. Oleh karena itu, diperlukan media tertentu untuk mempermudah dalam menguasai dan sebagai pemacu untuk menarik minat siswa dalam mempelajari hiragana. Penggunaan media kartu kuartet dirasa dapat mengatasi permasalahan diatas. Tujuan dalam penelitian ini adalah untuk mengetahui bagaimanakah pengaruh dari media kartu kuartet terhadap penguasaan hiragana siswa kelas X SMAN 6 Padang. Penelitian ini menggunakan penelitian eksperimen semu, dengan posttes tonly control group design. Sedangkan teknik penyampelan menggunakan teknik purposive sampling. Sampel dalam penelitian ini adalah siswa kelas X MIPA 1 dan X MIPA 5 dengan jumlah 69orang. Hasil analisis data, diperoleh thitung dengan ttabel taraf signifikan 0,05% adalah 2,00 dengan derajat kebebasan dk=n1+n2-2. Hal ini berarti thitung > ttabel (3,41 > 2,00). Maka hipotesis diterima, karena thitung > ttabel. Kata kunci: Hiragana, Media, Kartu Kuartet.