bluuebunny/crossref_metadata_2025_split huggingface dataset:
A systematic review of the outcomes of false‐positive results on newborn screening for congenital hypothyroidism
Authors: Rosettenstein Kerri R. , Lain Samantha J. , Wormleaton Nicola , Jack Michelle M. 
Published: July 2021

AbstractObjectivesThe potential of harm to infants or their parents from a false positive (FP) newborn screening (NBS) result for congenital hypothyroidism (CH) is often cited as an argument against lowering of screening thresholds for CH. This systematic review (SR) examines the evidence of harm and factors that possibly contribute.Study DesignPRISMA guidelines were followed and the protocol was registered online (Prospero, ID CRD42019123950, 20 August 2019) before the search was conducted. Multiple electronic databases and grey literature were searched. Articles were included/excluded based on predetermined eligibility criteria. Included articles were appraised for quality, using the relevant Critical Appraisal Skills Program (CASP) tool. Data were extracted and results were tabulated and summarised as part of a narrative synthesis.ResultsA total of six studies met the inclusion criteria. All were qualitative and three were based on the same cohort. Studies were published between 1983 and 1996. CASP appraisals scored 2/6 studies as moderate quality and 4/6 as low quality. Studies reported that FP results on CH screening may cause initial stress for parents and poorly defined behavioural disturbance in a small number of children, though these effects were generally not long‐lasting. Poor screening processes and inadequate communication with parents, increased the risk of harm to parents and children, from FP results.ConclusionThis SR found a small number of dated, qualitative studies of low to moderate quality, conducted soon after the initiation of NBS for CH. Conclusive evidence of the risks of harm from FP results and ways to mitigate harm, awaits further, well‐designed studies.
Risk of complications rise with coronary artery disease and diabetes mellitus after endobronchial valve placement in severe heterogeneous emphysema
Authors: Fernandez‐Bussy Sebastian , Kornafeld Anna , Labarca Gonzalo , Abia‐Trujillo David , Patel Neal M. , Johnson Margaret M. , Reisenauer Janani S. , Herth Felix J. 
Published: July 2021

AbstractBackgroundPatients with chronic obstructive pulmonary disease (COPD) commonly have comorbidities which may impact both symptoms and prognosis. Endoscopic lung volume reduction (ELVR) with endobronchial valves (EBV) is an innovative, effective and safe treatment for patients with severe emphysema who remain symptomatic despite optimal medical therapy.ObjectivesTo evaluate medical comorbidities associated with increased risk of complications after EBV procedure.MethodThis was a retrospective cohort review of patients with severe, heterogeneous emphysema who underwent Zephyr® EBV installation for ELVR. Demographics, baseline comorbidities, dyspnea scores, data regarding procedure, pulmonary function test, minimal clinically important difference and post‐EBV complications were recorded.ResultsOf a total 82 participants, 24 were identified as having a post procedure complication. There was a significant difference in the incidence of type 2 diabetes mellitus (T2DM) at baseline (25%) in complication group compared with non‐complication group (5.1%) (p value = 0.01). There was also a significant difference between baseline coronary artery disease (CAD) in those with (58.3%) or without (29.3%) complication (p = 0.01). Multivariate analysis of other covariables, identified associations of both CAD and T2DM with a heightened risk of complication (adjusted OR 4.19 CI: 1.23–14.2, p value = 0.02 and adjusted OR of 6.1 CI: 1.3–26.1, p value = 0.02 respectively).ConclusionsOur study found that patients with severe, heterogeneous emphysema who undergo ELVR utilizing Zephyr® EBV and suffer complications during post‐procedure period are more likely to have CAD or T2DM as baseline comorbidity. These data suggest that baseline CAD and T2DM might be predictive of increased risk of complications following ELVR with EBV.
What drives modern protected area establishment in Australia?
Authors: Hernandez Stephanie , Benham Claudia , Miller Rachel L. , Sheaves Marcus , Duce Stephanie 
Published: July 2021

AbstractProtected areas are a fundamental mechanism for ensuring the persistence of biodiversity. The strategic policy objectives set by governments for protected area land acquisition are strong determinants of biodiversity outcomes. An examination of these objectives is necessary to determine those most influential in designing protected area networks and understand why Australia's extinction rates exceed those elsewhere despite actively establishing protected areas over the past several decades. To examine spatio‐temporal trends in policy objectives for protected areas, we evaluated the strategic priorities in Federal, State, and Territory policy documents across Australia between 1992 and 2019 using thematic analysis. We classified priorities into seven themes: adequacy, Indigenous and cultural values; representation of ecosystem and species types; threatened species and their habitat;socialand recreational values;uniquevalues andavoiding threatening processes. We found that the representation of ecosystem and species types was the most prevalent theme in policy documents, and the least common theme wassocialand recreational values. We posit several reasons for this trend and warn that emphasizing extent, in terms of area or representativeness, may diminish the effectiveness, efficiency, and impact for biodiversity outcomes. We found that policies were generally supportive of the strategic identification of particular species or communities that would quantifiably benefit from protection (referred to asavoided loss). Risked‐based approaches to the establishment of protected areas are supported by modern conservation literature to enhance the protected area network's effectiveness. To maximize limited resources, we recommend that governments continue encouraging urgency to avoid species and habitat loss in their strategic priorities. This urgency should be accompanied by clear and consistent funding for on‐the‐ground actions which facilitate the socio‐ecological outcomes that characterize modern protected area policy.
Epidermotropic metastasis of squamous cell carcinoma of the tonsil: A case report with molecular confirmation
Authors: Li Philippa , Barbieri Andrea , Walther Zenta , McNiff Jennifer , Panse Gauri 
Published: July 2021

AbstractMetastasis of oropharyngeal squamous cell carcinoma (SCC) to skin is uncommon and portends a poor prognosis. Clinical history and histopathology are key to discerning between metastatic disease vs de novo SCC of the skin. We describe a case of an HPV+ tonsillar SCC in a 77‐year‐old male, with metastasis to the neck skin. This case is unique because of prominent in situ epidermal involvement on skin biopsy specimen, complicating the distinction between primary and secondary disease. The nature of the lesion was resolved using next‐generation sequencing of both the primary oropharyngeal SCC and skin lesion biopsy specimens. Both tumors showed identical ATR D1639G somatic mutations, while the skin lesion contained an additional POLE F1366L mutation. Clonal evolution of metastatic lesions is a well‐described phenomenon; comparing the genetic profiles of primary and metastatic specimens can be useful in evaluating the tumor origin as well as identifying targetable genetic aberrations.
Sex separation ofAedesspp. mosquitoes for sterile insect technique application: a review
Authors: Morán‐Aceves Brenda M. , Marina Carlos F. , Dor Ariane , Liedo Pablo , Toledo Jorge 
Published: July 2021

AbstractSeparation of the sexes is necessary for the application of the sterile insect technique (SIT) in mosquitoes due to the hematophagous habits and disease vector activity of the females. In this review we analyze the history, current status, and future perspectives for the development of genetic sexing strains (GSS) ofAedesmosquitoes (Diptera: Culicidae). Various genetic control methods for mosquitoes are reviewed, as are their need for sex‐separation methods. We focus on areas of opportunity where GSS developed with classical genetic methods can be used. Regulatory restrictions and social acceptance of various control methods are analyzed. We conclude that the development of GSS by classical methods represents the most viable option for separation of the sexes and the application of large‐scale SIT programs within an area‐wide integrated vector management (AW‐IVM) approach.
The relevance of the endocrine condition in microglia morphology and dendrite complexity of doublecortin‐associated neurons in young adult and middle‐aged female rats exposed to acute stress
Authors: Vega‐Rivera Nelly Maritza , González‐Monroy Edgar , Morelos‐Santana Erik , Estrada‐Camarena Erika 
Published: July 2021

AbstractMenopause, natural or surgical, might facilitate the onset of psychiatric pathologies. Some reports suggest that their severity could increase if the decline of ovarian hormones occurs abruptly and before natural endocrine senescence. Therefore, we compared the effects of ovariectomy on microglia's morphological alterations, the complexity of newborn neurons, and the animal's ability to cope with stress. Young adult (3 months) and middle‐aged (15 months) female Wistar rats were subjected to an ovariectomy (OVX) or were sham‐operated. After 3 weeks, animals were assigned to one of the following independent groups: (1) young adult OVX + no stress; (2) young adult sham + no stress; (3) young adult OVX + stress; (4) young adult sham + stress; (5) middle‐aged OVX + no stress; (6) middle‐aged sham + no stress; (7) middle‐aged OVX + stress; (8) middle‐aged sham + stress. Acute stress was induced by forced swimming test (FST) exposure. Immobility behavior was scored during FST and 30 min after; animals were euthanized, their brains collected and prepared for immunohistochemical detection of Iba‐1 to analyze morphological alterations in microglia, and doublecortin (DCX) detection to evaluate the dendrite complexity of newborn neurons. OVX increased immobility behavior, induced microglia morphological alterations, and reduced dendrite complexity of newborn neurons in young adult rats. FST further increased this effect. In middle‐aged rats, the main effects were related to the aging process without OVX or stress exposure. In conclusion, surgical menopause favors in young adult rats, but not in middle‐aged, the vulnerability to develop immobility behavior, retracted morphology of microglial cells, and decreased dendrite complexity of newborn neurons.
Migration and spawning behavior of the greater amberjack Seriola dumerili in eastern Taiwan
Authors: Tone Kazuki , Nakamura Yosuke , Chiang Wei‐Chuan , Yeh Hsin‐Ming , Hsiao Sheng‐Tai , Li Chun‐Huei , Komeyama Kazuyoshi , Tomisaki Masanori , Hasegawa Takamasa , Sakamoto Takashi , Nakamura Itsumi , Sakakura Yoshitaka , Kawabe Ryo 
Published: July 2021

AbstractGreater amberjack (Seriola dumerili) is an important fishery resource with a circumglobal distribution from tropical to temperate waters. Here, we investigated the spawning migration and habitat utilization of S. dumerili in the East China Sea (ECS). Archival tags were attached to 22 adult fish to examine their horizontal and vertical movements and estimate the spawning ground. S. dumerili were captured and released in the coastal waters of eastern Taiwan on November of 2016 and 2017. Information from seven pop‐up satellite archival tags and seven depth–temperature recorders was recovered. Almost all of the fish stayed in the Taiwanese exclusive economic zone. Most individuals moved from released site to the southern edge of the ECS and showed behavior associated with the topographic features in winter (November to December). These phenomena may be related to foraging and be driven by oceanographic features such as the seasonal monsoon and the Kuroshio. The fish then migrated to the south offshore area of Taiwan in January and February. During their southward migration, the fish experienced a slowly elevated water temperature regime (SETR), which is one of the environmental factors that induce final oocyte maturation. In the spawning season (February to April), tagged females exhibited continuous diel vertical movements (DVMs) after experiencing the SETR. These continuous DVMs were observed over a wide geographic range from north to south in the Kuroshio off eastern Taiwan. Our study demonstrated that the putative spawning ground of S. dumerili must extend further in a north–south direction than predicted in a previous study.
A worldwide and annotated database of evaporative water loss rates in squamate reptiles
Authors: Le Galliard Jean‐François , Chabaud Chloé , de Andrade Denis Otávio Vieira , Brischoux François , Carretero Miguel A. , Dupoué Andréaz , Gavira Rodrigo S. B. , Lourdais Olivier , Sannolo Marco , Van Dooren Tom J. M. 
Published: July 2021

AbstractMotivationThe understanding of physiological adaptations, of evolutionary radiations and of ecological responses to global change urges for global, comprehensive databases of the functional traits of extant organisms. The ability to maintain an adequate water balance is a critical functional property influencing the resilience of animal species to climate variation. In terrestrial or semi‐terrestrial organisms, total water loss includes a significant contribution from evaporative water loss (EWL). The analysis of geographic and phylogenetic variation in EWL rates must however account for differences in methods and potential confounding factors, which influence standard measures of whole‐organism water loss. We compiled the global and standardized SquamEWL database of total, respiratory and cutaneous EWL for 325 species and subspecies of squamate reptiles (793 samples and 2,536 estimates) from across the globe. An extensive set of companion data and annotations associated with the EWL measurements of potential value for future investigation, including metabolic rate data, is provided. We present preliminary descriptive statistics for the compiled data, discuss gaps and biases, and identify promising avenues to update, expand and explore this database.Main types of variables containedStandard water loss rates, geographic data, metabolic rates.Spatial locationGlobal.Time periodData were obtained from extant species and were collected between 1945 and 2020.Major taxaReptilia, Squamata including lizards, snakes and amphisbaenians.Level of measurementsIndividual samples of animals from the same species, locality, age class and sex category.Software formatcsv.
Diversity patterns and drivers of methanotrophic gene distributions in forest soils across a large latitudinal gradient
Authors: Kou Yongping , Zhao Wenqiang , Liu Yanjiao , Wu Yanhong , Xiao Jiangtao , Wang Xiaohu , Bing Haijian , Liu Qing 
Published: July 2021

AbstractAimMethane oxidation driven by soil aerobic methanotrophs exhibits the largest methane sink capacity in forest ecosystems and plays important roles in the alleviation of global warming. Many studies have revealed the distribution of methanotrophs in forest ecosystems at field scales; however, the biogeographical patterns of methanotrophic communities and related ecological processes and the drivers shaping methanotrophic communities at a large scale remain poorly understood.LocationChina’s forests.Time period2012–2013.Major taxa studiedMethanotrophic communities.MethodsWe evaluated the geographical distributions of soil methanotrophic communities across 26 forests along a 4,000‐km north–south transect in China using a MiSeq high‐throughput sequencing technique. The assembly processes and drivers of the methanotrophic communities were evaluated using the phylogenetic null model approach and structural equation modelling, respectively.ResultsThe results showed that the upland soil cluster α (USCα) and ammonia‐ oxidizing bacteria‐related (AOB‐rel) methanotrophs were the most abundant taxa in forest soils and exhibited contrasting distributions of relative abundance across different climate zones, suggesting that they occupy different environmental niches. The α‐diversity pattern of the soil methanotrophic community followed a hump‐shaped pattern along a large latitudinal gradient. Statistical analyses suggest that aridity and vegetation productivity [here represented with the normalized difference vegetation index (NDVI)] are major drivers of the α‐diversity of the methanotrophic community, whereas soil pH is the key environmental factor shaping the β‐diversity of the methanotrophic community at a large scale. Additionally, the community assembly of the methanotrophs primarily resulted from deterministic processes, among which heterogeneous selection had a greater contribution in shaping the methanotrophic community than homogeneous selection.Main conclusionsOur study provides new insights into the biogeographical distributions, assembly processes and ecological predictors of the methanotrophic community in forest soil at a large scale. These results can be used to improve simulation models for the better prediction of ecosystem functions under predicted global changes.
Surgical approach in the management of ear keloids: our experience with 30 patients
Authors: Madura Chandraiah , Nayak Preethi Balakrishna , Raj Pavan R. , Chandrashekar Byalakere Shivanna 
Published: July 2021

AbstractBackgroundEar keloids are benign, fibrous proliferations due to excessive collagen synthesis and deposition. It is a popular practice to pierce earlobes for decorative earrings and adornment; this might trigger the keloid process. Although there are varied treatment modalities, it is unsatisfactory and has always been a challenge. The aim is to evaluate the efficacy of surgical treatment with intralesional therapy in auricular keloids.MethodsWe included 30 patients with 45 keloids over the ear. Patients were evaluated (including detailed history, complete physical and local examination), and photographs and written informed consent were taken. They were treated with: excision and closure, intralesional and/or surface cryotherapy, ablative laser, intralesional steroids, and 5‐fluorouracil. Excision and closure, and intralesional cryotherapy were done under local anesthesia. Closure was done after intramarginal excision with or without raising auto flaps, followed by intraoperative intralesional steroids to margins. Recurrence was assessed at 3 weeks, 3 months, 6 months, and 1 year.ResultsThe age group of patients ranged from 14 to 57 years. A total of 32 out of 45 (71.1%) keloids were excised and were combined with intraoperative and postoperative intralesional steroid injection, with sessions depending on the patient's response. Eight (17.7%) and five (11.1%) keloids were treated using intralesional cryotherapy and only intralesional steroids, respectively. A total of 16.6% of patients had recurrence with one patient having recurrence of bilateral earlobes keloid.ConclusionKeloidectomy with intraoperative and postoperative intralesional steroid injections has been very effective in the treatment of ear keloids. Different treatment modalities act synergistically, but excision surgery gives good results as it aims at maintaining ear architecture.
Drug‐induced Grover’s disease: a case report and review of the literature
Authors: Awe Olufolakemi , Pavlidakey Peter , Kole Lauren , Kissel Rebecca 
Published: July 2021

AbstractBackgroundGrover’s disease (GD) is a relatively rare transient dermatosis that can be idiopathic or acquired. Acquired GD may occur secondary to internal triggers such as medications and malignancies and external factors such as friction.ObjectiveThe purpose of this report is to describe the clinical and histological presentation of drug‐induced Grover's disease (DIGD) and discuss potential pathogenic mechanisms.MethodsA systemic review of the literature was performed to identify medications implicated in DIGD.ResultsWe identified 13 reports of patients with DIGD. Most patients presented with a papular or papulovesicular morphology involving the trunk and extremities. Pruritus was the most common symptom. The majority of the offending agents were cancer therapeutics. Discontinuation of the culprit medication was sufficient for rash clearance and symptom resolution in most cases.ConclusionThe overlap in morphology and associated symptoms in DIGD and GD makes the diagnosis of DIGD challenging and has potentially led to underdiagnosis. However, in cases of more extensive involvement and treatment recalcitrance, a drug‐induced eruption should be considered.
Evaluating Online Learning Practice in the Interior Design Studio
Authors: Alawad Abeer 
Published: July 2021

AbstractThe COVID‐19 pandemic has led most countries to adopt precautionary isolation measures to prevent the spread of the virus. In this context, teaching through online studios has become a necessity. This study aimed to identify students’ practical experiences in fully online interior design studios and determine whether these experiences differ according to students’ educational levels to help improve and ensure the quality of online design education. The participants were 69 female interior design students. A questionnaire was applied using five axes, namely virtual learning environment, curriculum and illustration tools, students’ experiences, interaction with the teacher, and overall satisfaction and hopes. The results showed that the newer students had a positive experience with the online design studio, while there were statistically significant differences between the answers of students at different levels. It was noticeable that the students’ experience on all axes was different, and the level of satisfaction among the new students in Studio 2 was higher than among the students nearing graduation in Studio 6. The findings of this study will benefit the academic programme and technology developers in creating a suitable online learning environment for each profession.
Nanopore sequencing reveals full‐length Tropomyosin 1 isoforms and their regulation by RNA‐binding proteins during rat heart development
Authors: Cao Jun , Routh Andrew L. , Kuyumcu‐Martinez Muge N. 
Published: July 2021

AbstractAlternative splicing (AS) contributes to the diversity of the proteome by producing multiple isoforms from a single gene. Although short‐read RNA‐sequencing methods have been the gold standard for determining AS patterns of genes, they have a difficulty in defining full‐length mRNA isoforms assembled using different exon combinations. Tropomyosin 1 (TPM1) is an actin‐binding protein required for cytoskeletal functions in non‐muscle cells and for contraction in muscle cells. Tpm1 undergoes AS regulation to generate muscle versus non‐muscle TPM1 protein isoforms with distinct physiological functions. It is unclear which full‐length Tpm1 isoforms are produced via AS and how they are regulated during heart development. To address these, we utilized nanopore long‐read cDNA sequencing without gene‐specific PCR amplification. In rat hearts, we identified full‐length Tpm1 isoforms composed of distinct exons with specific exon linkages. We showed that Tpm1 undergoes AS transitions during embryonic heart development such that muscle‐specific exons are connected generating predominantly muscle‐specific Tpm1 isoforms in adult hearts. We found that the RNA‐binding protein RBFOX2 controls AS of rat Tpm1 exon 6a, which is important for cooperative actin binding. Furthermore, RBFOX2 regulates Tpm1 AS of exon 6a antagonistically to the RNA‐binding protein PTBP1. In sum, we defined full‐length Tpm1 isoforms with different exon combinations that are tightly regulated during cardiac development and provided insights into the regulation of Tpm1 AS by RNA‐binding proteins. Our results demonstrate that nanopore sequencing is an excellent tool to determine full‐length AS variants of muscle‐enriched genes.