bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Freezing Process of Red Snapper (Lutjanus malabaricus) with the Air Blast Freezing (ABF) Method at PT. Inti Luhur Fuja Abadi, Pasuruan, East Java
Authors: Scriptura Gabriel Zola , Masithah Endang Dewi 
Published: September 2021

Fish is a protein source that is very potential and needed by humans. One of the fish that has a high enough nutrient content is Red Snapper (Lutjanus malabaricus). Snapper fish contains 92 kilocalories of energy, 20 grams of protein, 0 grams of carbohydrates, 0.7 grams of fat, 20 milligrams of calcium, 200 milligrams of phosphorus, and 1 milligram of iron. The high water content is one potential that must be considered because it is a good medium for microbial growth. Therefore efforts are needed to maintain the nutritional content, freshness, taste, and extend the shelf life through preservation, one of which is by freezing. The Air Blast Freezing method is one of the freezing methods that uses a combination of low temperatures which are temperatures of -35℃ to -40℃ by blowing cold air quickly towards the product. This method has the advantage of one of which is relatively easy operation. The purpose of this field work practice is to know the process of freezing Red Snapper (Lutjanus malabaricus) with the Air Blast Freezing (ABF) method and the obstacles in the production of Red Snapper (Lutjanus malabaricus) using the Air Blast Freezing (ABF) method. The stages of the production process of Red Snapper (Lutjanus malabaricus) are the reception of raw materials, washing I, sorting, washing II, weighing, washing III, preparation, freezing with the Air Blast Freezing (ABF) method, glazing, packaging, storaging, stuffing. The freezing process with the Air Blast Freezing (ABF) method uses a temperature of -35℃ to -40℃ for 6-8 hours so that the plant can produce as much as 3 tons in one day. Obstacles to the freezing process at PT ILUFA are raw materials that contain parasites. Factors that influence the freezing process include raw materials during the reception and monitoring process during the freezing process in the ABF engine.
Technique of Increasing Calcium Carbonate (CaCO3) in White Shrimp (Litopenaeus vannamei) Feed in Kasetsart University, Bangkok
Authors: Nurfaidah Elva , Agustono Agustono 
Published: September 2021

White shrimp (Litopenaeus vannamei) culture has grown rapidly in Indonesia since early 2000s. White shrimp have stronger resistance to WSSDV attacks, and are euryhaline. In it’s natural habitat, this shrimp was found in waters with a range of 0.5-40 ppt salinity. White shrimp grow optimally at 15-25 ppt salinity, even still suitable at 5 ppt salinity. This capability provides the opportunities to culture these commodities in inland water. White shrimp need inorganic elements or minerals for their living processes. Calcium is one of the main needs of shrimp that is closely related to the development and maintenance of skeletal systems and plays a role in some physiological processes of the body.The requirement for calcium in shrimp is increasing when it is cultivated in fresh water.This Internship aims to find out the technique of adding calcium carbonate (CaCO3) in feed, used for the white shrimp culture in fresh water. In this field practice, the addition of calcium carbonate in a level of 2% refers to the requirement of white shrimp for optimal growth. This was based on the calcium requirements of shrimp in the amount of 1-2%. The method was repelleting, by grinding feed and calcium using mortar and pestle, then filtered to get the smoothest part. Then mix calcium with 10 mL of water and mix it with pellets evenly. Then the mixture is air dried and mashed again and weighed according to the required dose of feed. The addition of calcium in the feed affects the growth of shrimp, because calcium is one of the most important anorganic mineral for the growth and development of exoskeleton, regulates blood clotting, heart rate, kidneys, nerves, the activity of several enzymes, and cell function
3. Lactobacillus casei Fermented Milk as a Treatment for Diabetes in Mice (Mus musculus)
Authors: Sari Pratiwi Purnama , Nurliana Nurliana , Hasan M. , Sayuti Arman , Sugito Sugito , Amiruddin Amiruddin 
Published: September 2021

The study aimed to find out the effect of Lactobacillus casei fermented milk as diabetic drug (Therapeutic). Mice were 3-4 month old, male and body weight ranged 20-30 g. This Research used Completely Randomized Design with factorial pattern, consisted of dose and duration of feeding. Treatments were divided into four treatment, P1 (no treatment), P2 (mice were induced with alloxan), P3 (mice were induced with alloxan and fed with fermented milk L. casei with 0.5 mL/mice) and P4 (mice were induced with alloxan and fed with Lactobacillus casei fermented milk 1 mL/mice). Provision of L. casei fermented milk provided at libitium and L. casei fermented milk given for 7 and 14 days. Blood were collected from Vena lateralis and dropped on the strip test Easy Touch GGHb. The results showed that not only dose administration of L. casei fermented milk had significantly decrease (P0.01) of glucose levels in mice, but also duration of treatment. It can be concluded that the administration of fermented milk L. casei can be used as a complement therapy for diabetic mice
20. The Histological Structure of Tegal Duck (Anas javanicus) Spleen at Different Age
Authors: Hafizsha Nabila Latifa , Budiman Hamdani , Hamny Hamny , Masyitha Dian , Iskandar Cut Dahlia , Balqis Ummu , Jalaluddin Muhammad 
Published: September 2021

The aim of the study was to determine the histological structure of tegal duck (Anas javanicus) spleen according to ages variation. One month, 2 month and 3 months of tegal duck were used as the sample of this study. The spleen of each duck was analyzed by Hematoxylin-Eosin (HE) method, then observed under light microscope. The result of this study performed there were the differences of histological structure of the ducks spleen. The capsule thickness had increased along with increasing age, which was 16.0 ± 1.0 µm; 25.3 ± 2.5 µm; and 29.0 ± 1.0 µm. The boundary between the two pulp in the tegal duck 3 months old more obvious than 1 month or 2 months old. The diameter of white pulp had increased in the oldest duck. It can be concluded that the development of spleen histological structure was more perfect with the increasing age of tegal duck.
17. Knowledge of Understanding the Relationship of Sexual desire’s Symptoms of Lust with the Success of Artificial Insemination on Cattle Farmer at the Lengayang subdistrict, Pesisir Selatan district, West Sumatera
Authors: Dilla Nida Ulfa , Thasmi Cut Nila , Hamdan Hamdan 
Published: September 2021

This study aimed to find out the understanding of cattle farmer in Lengayang, Pesisir Selatan West Sumatera about the relation of sexual desire symptom with the success rate of artificial insemination on cattle. The respondents of this study were the cattle farmer on Lengayang base on the result of the method of this study was field survey. The data were collected interview the farmer using structural questioner form. The data consist of secondary and primary data. The secondary data were obtained from Animal Husbandry departement and Animal Health on Pesisir Selatan. Data were analyzed descriptively. Result showed that from 100 respondents there were 15 respondents from Lakitan village, 16 respondent from East Lakitan, 14 respondents from East Kambang, 35 respondents from West Kambang, 9 respondents from Kambang and 11 respondents from North Kambang. Based on the study result the level of farmers knowledge about understanding relation sexual desire the symptoms with the success of artificial insemination in cattle is 2% excellent, 33% good, 56% fairly good and 9%. Less Good. Based on Regression test formal education, non-formal education and experience effect of to the knowledge of the success of artificial insemination 6.6%
12. The Effect Of Neem Leaves (Azadirachta Indica) Extract To The Blood Profile Of Male Rat (Rattus Norvegicus) Infected With Trypanosoma Evansi Of Krueng Raya Isolat
Authors: Agusty Atika , Fahrimal Yudha , Lubis Triva Murtina , Hambal Muhammad , NA Zuhrawaty , Sayuti Arman 
Published: September 2021

This study aimed to determine the ability of neem leaf extract to maintain the normal blood profile of white male rat (Rattus norvegicus) infected with Trypanosoma evansi. A total of 24 male white rats were divided into 6 groups. The K0 group as the negative control received no T. evansi infection and no neem leaf extract. Furthermore, K1 group (positive control) only infected with T. evansi, K2 and K3 were given neem leaf extract 50mg/kg BW and 100mg/kgBW, while K4 and K5 were given neem leaf extract 400mg/KgBW and K5 800mg/kgBW. The neem leaf extract was administered for 3 consecutive days after infection established. The blood of the rats was collected to determine the amount of erythrocyte and leukocyte, hemoglobin level and hematocrit value. The data were analyzed using Analysis of Variance (ANOVA) through SPSS for Windows 16.0. The averages (+SD) of erythrocyte of K0, K1, K2, K3, K4, and K5 were 5,64±0,57; 2,31±1; 1,93±0; 0; 2,55±0,33; and 2,56±0,48. The amount of leukocyte were 4,46±1,09; 4,45±1,91; 5,25±0; 0; 8,42±1,66; and 8,14± 5,17. The value of hemoglobin were 12,00±0,47; 10,50±0,57; 4,30±0; 0; 5,60±0,20; and 9,03±0,66. The level of hematocrit 38,00±1,83; 25,00±9,90; 15±0; 0; 18,00±2,65, and 20,75±1,71. The result showed that the administration of neem leaf extract with doses 400mg/kgBW and 800mg/kgBW were difference significantly (P0.05) compare to the positive control of rats, but could not equalize blood profile of uninfected white male rats
20. The Isolation of Salmonella sp. on quail eggs (cortunxi-cortunix japonica) that failed to hatch in Garot, Darul Imarah Subdistrric, Aceh Besar
Authors: Suryandari Latifa , Erina Erina , Darniati Darniati , Safika Safika , Asmilia Nuzul , Salim M. Nur 
Published: September 2021

The study aimed to isolate the Salmonella sp on quail eggs that failed to hatch in Garot village, Darul Imarah Subdistrict, Aceh Besar. A total of thirty quail eggs that failed to hatch were examined in microbiology laboratory of Veterinary Medicine Faculty, Syiah Kuala University. Modification of Carter method was used in this study to isolated samples. The eggs were opened for taking the embryo and then it was swabbed with sterile swabs. The swab was put into Selenite Cystine Broth (SCB) and incubated at 37o C for 24 hours. The breed was then transferred onto Salmonella Shigella Agar (SSA). The growing Bacteria were observed for their morphology and Gram staining to make sure the bacteria belonged to the negative Gram. The result showed that 10 of the 30 of quail eggs that failed to hatch were positively infected with Salmonella sp. Based on this study, it can be concluded that the salmonella sp.can be isolated from quail eggs which were not hatched in the farm of Garot, Darul Imarah Subdistrict, Aceh Besar
15. Potency of Methanolic Extract of Sernai Stems (Wedelia biflora) as Analgesic on Mice (Mus musculus)
Authors: Rahmatia Annisa Rizka , Rinidar Rinidar , TR T. Armansyah , Rosmaidar Rosmaidar , Harris Abdul , Fahrimal Yudha 
Published: September 2021

The purpose of this research was to determine the potency of methanolic extract of sernai stem (Wedelia bilflora) as analgesic to mice (Mus muculus) compared to ibuprofen. This research use split-plot method with 5 treatments and 5 replications. Group P0 as negative control was given aquadest, P1 as positive control was given ibuprofen, while P2, P3, and P4 were given the extract with dosage of 40, 50, and 60 mg/kg bw, respectively. The pain analysis of mice was conducted using hotplate method and the observation was carried out at 0 minute, 30 minutes, 60 minutes, 90 minutes, and 120 minutes. The data was analyzed using analysis of varians (Anova) followed by Duncan’s test. The results of this research showed that P1, P2, P3 and P4 had significant difference (P0,05) from P0. P1 did not have significant difference (P0,05) from P2 but it was different from P3 and P4 (P0,05). From this result we can conclude that methanolic extract of sernai stem was potential as analgesic, dosage of 40 mg/kg bw was equal to ibuprofen, while 50 mg/kg bw and 60 mg/kg bw showed better effect than ibuprofen.
21. Physical Quality and Organoleptik of Beef Added with Curry Leaf (Murraya koenigii) Infuse
Authors: Murliana Baida , Rastina Rastina , Ismail Ismail , Nurliana Nurliana , TR T. Armansyah , Aliza Dwinna 
Published: September 2021

The aims of this research were to compare the physical charateristic of meat with or without the addition of curry leaf and to find out the organoleptic of meat added with curry leaf. This research used complex randomized design method. 500 grams beef sample were cut into square-shape at 2x2x2 cm and divide into 3 groups and then added a curry leaf infuse with different concentration of 0%, 25%, and 50%. Then, the meat was boiled at 70-80oC for 45 minutes. The parameters observed were pH, water holding capacity, cooking loss, and organoleptic (colour, taste, and aroma). The result showed that the use of curry leaf infuse in various concentration did not have significant effect on pH, water holding capacity, and cooking lost, but significantly effect (P0,05) on organoleptic value. Fresh meat that was added the curry leaf infuse 50% was well-liked for the colour, taste, and aroma compare to the meat without the addition of curry leaf infuse.
19. Histological of Thymus in Tegal Duck (Anas javanicus) at Different Ages
Authors: Budiman Hamdani , Septian Anugrah , Hamny Hamny , Zainuddin Zainuddin , Iskandar Cut Dahlia , Sabri Mustafa , Hambal Muhammad , Harris Abdul 
Published: September 2021

The aim of this research was to observe the histological of the thymus in tegal duck (Anas javanicus) according to the variation of ages. The animals used in this research were one month, two months and three months old of tegal duck. The thymus of each duck was analyzed by Hematoxylin-Eosin (HE) method and then observed under a light microscope. The result of the research performed differences in thymus structure of these three ages. The cortex had increased at the age of 2 months and decreased at the age of 3 months. The longest diameter of medulla at the age of 3 months was followed by ages 2 months and 1 month of tegal ducks. The distribution of Hassall's body had increased at every age level. So, the cortex thickness decreased at the age of 2 months to 3 months, while the diameter of the medulla was getting longer.
2. The Effect Of Sipatah-patah (Cissus quadrangularis Salisb) Extract On The Femur Bone Density of White Rat (Rattus norvegicus) with Model Ovariectomy
Authors: Sabri Mustafa , Ayumi Debby Novita , Jalaluddin Muhammad , Hamny Hamny , Iskandar Cut Dahlia , Herrialfian Herrialfian 
Published: September 2021

This research aimed to determine the effect of giving Sipatah-patah extract toward the histopathological and femur bone growth of white rat that was ovariectomized. The experimental animals that were used were 12 white rats divided into 4 treatment group with 3 repetitions. K0 was the ovariectomized rat without giving Sipatah-patah extract (ESP); K1, K2 and K3 were ovariectomized rats which were given Sipatah-patah extract with multilevel doses of 500 mg/kg BW, 700 mg/kg BW and 900 mg/kg BW for 30 days. On the 31st day, rats were euthanized using chloroform and os femur that was taken to being made into histological preparation. There was the decrease in the bones density of the K0 group which is characterized by thinning of trabecular structure, there were lots of osteoclast cells on the edge of the trabecular and lower density of active osteoblasts and passive osteoblasts. The rat of group K1 and K2 showed an improvement on the trabecular structure and lower osteoclast than group K0. The rat of group K3 had a visible improvement of the most congested trabecular structures, cohesive with the most density cell of active osteoblasts than the other groups. The result of this research concluded that the giving of Sipatah-patah extract doses 900 mg/kg BW showed a higher density of trabecular and active osteoblasts than the control group, K1 and K2 on the white rat bones that were ovariectomized.
17. Oxytetracycline Activities To Staphylococcus aureus Biofilm Inhibition Of Aceh Cattle Preputium Isolate
Authors: Dewi Maryulia , Darmawi Darmawi , Helmi T. Zahrial , Erina Erina , Gani Basri , Eliawardani Eliawardani , Azhar Azhar 
Published: September 2021

The aim of this study was to see the antimicrobial activities of oxytetracycline to the inhibition of Staphylococcus aureus biofilm of Aceh’s cattle preputium isolates. The parameters measured in this study were the percentage of antimicrobial and biofilm inhibitory power. The antimicrobial activities were tested on Nutrient Broth using U-bottom polystyrene microplate 96 wells. The Biofilm stained using 1% of crystalviolet. Then, Optical Density (OD) read at λ 595 nm. The results showed that the oxytetracycline of 10%, 20% and 30% (μg / μL) had the antimicrobial abilities of 28.60% ± 13.23%; 36.30% ± 5.37%; and 57.70% ± 2.23%. In addition, The biofilm inhibitory capacity of oxytetracycline 10%, 20% and 30% (μg / μL) were 2.20% ± 8.77%; 2.70% ± 4.17%; and 3.73% ± 14.14%. Therefore, it can be concluded that the oxytetracycline had the ability to inhibit the biofilm of Staphylococcus aureus.
40. Knowledge and Perception of Cervical Cancer
Authors: Halifah Elka , Mutiara Erna , Masniari Lubis Ria 
Published: September 2021

Cervical cancer is a type of cancer which ranks the second of whole incident of cancer in women. Cervical cancer can metastatic to other organs and can cause death. The objective of the research was to knowlage and perception about cervical cancer. The type of research was a survey with descriptive design. The population of women in Banda Aceh and 60 of them were used as the samples using accidental sampling technique. The data were collected by interview and analyzed by using univariat. There were influence of poor knowledge 63,3%, and 75,0% negative perception about cervical cancer. It is recommended that the local Government, Health Office and Indonesian Cancer Foundation in Nanggroe Aceh Darussalam Province provide convenience in service counseling in order to increase knowledge. The movement of early detection program (papsmear) need to be improved so that women sexually active are willing to examine themselves to the health service so that there will be no lateness in having treatment.
31. Histological of Tractus Digestivus of Domestical Catfish (Clarias batracus)
Authors: Irfandi Aliman , Iskandar Cut Dahlia , Zainuddin Zainuddin , Masyitha Dian , Fitriani Fitriani , Hamny Hamny , Panjaitan Budianto 
Published: September 2021

Domestical catfish is grouped into omnivora, although the fish is tended into carnivora. The aim of the present study was to investigate histology of alimentary tract of domestical catfish (Clarias batrachus). In order to determine alimentary tract of domestical catfish, oesophagus, gastric and intestines is investigated by Haematoxilin Eosin. Histology exploration was performed each layer of alimentary tract’s wall consist of lamina epithelia, propria, and muscularis mucosa. Collagen connective tissue was developed tunica submucosa. The muscle of tunica muscularis was performed circular and elongated. Dense connective tissue and blood vessels was located in the tunica serosa. Histology exploration revealed has stratified squamous epithelium, mucosit cells, eosinophilic club cells on the lamina epithelia, and taste buds on the lamina propria in oesophagus. Gastric was establised simple prismatic cells, and glands of gastric on the lamina propria. Furthermore intestine was showed villi and goblet cells. There were not showed Brunner and Liberkhun gland. In conclusion, histology of alimentary tract was revealed from tunica mucosa, submucosa, and serosa.
Parasitemia of Male White Mice (Mus musculus L.) DDY Strain Infected with Trypanosoma evansi Pidie and Pemalang Isolate
Authors: Sari Febiola Rama , Fahrimal Yudha , Balqis Ummu , Subekti Didik T , Wardana Aprilia 
Published: September 2021

This study aimed to find out the parasitemia of DDY white mice infected with T. evansi of Pidie and Pemalang isolates obtained from Bbalitvet Bogor. A total of 12 mice were divided into 3 treatment groups. Group 1 (K1) without any treatment, group 2 (K2) was inoculated with 104 T. evansi Pidie isolate, and group 3 (K3) was inoculated with 104 T. evansi Pemalang isolate. Parasitemia examination was carried out every two days and the level of parasitemia was observed as well. Parasitemia examination was conducted until all mice died. Parasitemia of mice infected with Pidie isolate was characterized by rapid rise of parasitemia in blood (107-108/mL of blood) in a short time (2-4 days) since first parasitemia was detected and followed by death at day 4. The parasitemia of mice infected with Pemalang isolate increased in the blood (108-109/mL of blood) on day 4 and maintained for a few more days and then fluctuated for a few more days before the animal was dead. In conclusion, there was the difference in parasitemia level between Pidie and Pemalang isolates. Key words: Trypanosoma evansi, parasitemia, Pidie isolate, Pemalang isolate
Constructing mRNA, miRNA, circRNA and lncRNA Regulatory Network by Analysis of Microarray data in Breast Cancer
Authors: Hassani Bita , Mollanoori Hasan , Pouresmaeili Farkhondeh , Asgari Yazdan , Ghafouri-Fard Soudeh 
Published: September 2021

Abstract
Background. Luminal tumors are the utmost frequent subtype of breast cancer (BC). Despite luminal BC has relatively good prognosis, in a subset of patients, disease relapse occursto endocrine therapy ;hence, there is a critical need to identify new strategies to promote the early detection and more effective therapies. Noncoding RNAs including microRNAs, long noncoding RNAs, and circular RNAs can interact with and modulate each other via diverse molecular mechanisms and make a complicated regulatory network.ncRNAs participate in diverse biological processes and disorders such as breast tumors. Therefore, understanding their regulatory mechanisms allow to develop new field of research and therapeutic options for BC patients.Methods. In this study, BC-specific RNA expression profiles including mRNAs, miRNAs, lncRNAs, and circRNAs were retrievedfrom Gene Expression Omnibus microarray datasets, and differentially expressed(DE) items were obtained. Disease ontology, functional and pathway enrichment analyses were executed. The protein-protein interaction network was constructed, and hub mRNAs were extracted.The prognostic value of hub mRNAs in patients of BC were performed. Subsequently, a ceRNA network was established.Results. In total, 691 DE genes, 122 DE lncRNAs, 60 DE miRNAs, and 38 DE circRNAs in breast tumor samples were compared with normal samples. Subsequently, 12 hub-genesincluding FOXO3, RHOA, EZH2, KIT, HSP90B1, NCOA3, RAC1, IGF1, CAV1, CXCR4, CCNB1, and ITGB1 were screened from the network. Kaplan-Meier Plotter results revealed that FOXO3 and RHOA were a suitable prognostic marker for patients with breast cancer. Finally, we determined possible ncRNAs (circ0007535, circ0002727, circ0005240, circ0014130, circ0044927, circ0007001, circ0089153,NORAD, MALAT1, TUG1, ZFAS1, OPI5-AS1, miR183,miR182, miR101, miR200c, miR200b, miR149, miR342, and miR1207) which could crosstalk with each other to regulateFOXO3 and RHOA through different regulatory patterns. Conclusion. These data might improve our perception of the breast tumorigenesis and could develop new field of research and therapeutic options for BC patients.