bluuebunny/crossref_metadata_2025_split huggingface dataset:
Single-Cell Profiling of the Antigen-Specific Response to BNT162b2 SARS-CoV-2 RNA Vaccine
Authors: Kramer Kevin J. , Wilfong Erin M. , Voss Kelsey , Barone Sierra M. , Shiakolas Andrea R. , Raju Nagarajan , Roe Caroline E. , Suryadevara Naveenchandra , Walker Lauren , Wall Steven C. , Paulo Ariana , Schaefer Samuel , Dahunsi Debolanle , Westlake Camille S. , Crowe James E. , Carnahan Robert H. , Rathmell Jeffrey C. , Bonami Rachel H. , Georgiev Ivelin S. , Irish Jonathan M. 
Published: July 2021

SUMMARYRNA-based vaccines against SARS-CoV-2 are critical to limiting COVID-19 severity and spread. Cellular mechanisms driving antigen-specific responses to these vaccines, however, remain uncertain. We used single-cell technologies to identify and characterized antigen-specific cells and antibody responses to the RNA vaccine BNT162b2 in longitudinal samples from a cohort of healthy donors. Mass cytometry and machine learning pinpointed a novel expanding, population of antigen-specific non-canonical memory CD4+and CD8+T cells. B cell sequencing suggested progression from IgM, with apparent cross-reactivity to endemic coronaviruses, to SARS-CoV-2-specific IgA and IgG memory B cells and plasmablasts. Responding lymphocyte populations correlated with eventual SARS-CoV-2 IgG and a donor lacking these cell populations failed to sustain SARS-CoV-2-specific antibodies and experienced breakthrough infection. These integrated proteomic and genomic platforms reveal an antigen-specific cellular basis of RNA vaccine-based immunity.ONE SENTENCE SUMMARYSingle-cell profiling reveals the cellular basis of the antigen-specific response to the BNT162b2 SARS-CoV-2 RNA vaccine.
The transcriptional corepressor CTBP-1 acts with the SOX family transcription factor EGL-13 to maintain AIA interneuron cell identity in C. elegans
Authors: Saul Josh , Hirose Takashi , Robert Horvitz H. 
Published: July 2021

AbstractCell identity is characterized by a distinct combination of gene expression, cell morphology and cellular function established as progenitor cells divide and differentiate. Following establishment, cell identities can be unstable and require active and continuous maintenance throughout the remaining life of a cell. Mechanisms underlying the maintenance of cell identities are incompletely understood. Here we show that the gene ctbp-1, which encodes the transcriptional corepressor C-terminal binding protein-1 (CTBP-1), is essential for the maintenance of the identities of the two AIA interneurons in the nematode Caenorhabditis elegans. ctbp-1 is not required for the establishment of the AIA cell fate but rather functions cell-autonomously and can act in later larval stage and adult worms to maintain proper AIA gene expression, morphology and function. From a screen for suppressors of the ctbp-1 mutant phenotype, we identified the gene egl-13, which encodes a SOX family transcription factor. We found that egl-13 regulates AIA function and aspects of AIA gene expression, but not AIA morphology. We conclude that the CTBP-1 protein maintains AIA cell identity in part by utilizing EGL-13 to repress transcriptional activity in the AIAs. More generally, we propose that transcriptional corepressors like CTBP-1 might be critical factors in the maintenance of cell identities, harnessing the DNA-binding specificity of transcription factors like EGL-13 to selectively regulate gene expression in a cell-specific manner.
BRET-based self-cleaving biosensors for SARS-CoV-2 3CLpro Inhibitor Discovery
Authors: Hou Ningke , Peng Chen , Zhang Lijing , Zhu Yuyao , Hu Qi 
Published: July 2021

AbstractThe 3C-like protease (3CLpro) of SARS-CoV-2 is an attractive drug target for developing antivirals against SARS-CoV-2. A few small molecule inhibitors of 3CLpro are in clinical trials for COVID-19 treatments and more inhibitors are being developed. One limiting factor for 3CLpro inhibitors development is that the cellular activities of such inhibitors have to be evaluated in a Biosafety Level 3 (BSL-3) or BSL-4 laboratory. Here, we design genetically encoded biosensors that can be used in BSL-2 laboratories to set up cell-based assays for 3CLpro inhibitor discovery. The biosensors were constructed by linking a green fluorescent protein (GFP2) to the N-terminus and a Renilla luciferase (RLuc8) to the C-terminus of SARS-CoV-2 3CLpro, with the linkers derived from the cleavage sequences of 3CLpro. After over-expression of the biosensors in HEK293 cells, 3CLpro can be released from GFP2 and RLuc by self-cleavage, resulting in a decrease of the bioluminescence resonance energy transfer (BRET) signal. Using one of these biosensors, pBRET-10, we evaluated the cellular activities of several 3CLpro inhibitors. These inhibitors restored the BRET signal by blocking the proteolysis of pBRET-10, and their relative activities measured using pBRET-10 were consistent with their anti-SARS-CoV-2 activities reported previously. We conclude that the biosensor pBRET-10 is a useful tool for SARS-CoV-2 3CLpro inhibitor discovery. Furthermore, our strategy can be used to design biosensors for other viral proteases that share the same activation mechanism as 3CLpro, such as HIV protease PR and HCV protease NS3.HighlightsSensitive cell-based biosensors for 3CLpro inhibitor discovery in BSL-2 laboratories.The BRET-based self-cleaving biosensors mimic the in vivo autoproteolytic activation of 3CLpro.Similar biosensors can be designed for other self-cleaving proteases, such as HIV protease PR and HCV protease NS3.
A non-functional copy of the salmonid sex determining gene (sdY) is responsible for the “apparent” XY females in Chinook salmon, Oncorhynchus tshawytscha
Authors: Bertho Sylvain , Herpin Amaury , Jouanno Elodie , Yano Ayaka , Bobe Julien , Parrinello Hugues , Journot Laurent , Guyomard René , Muller Thomas , Swanson Penny , McKinney Garrett , Williamson Kevin , Meek Mariah , Schartl Manfred , Guiguen Yann 
Published: July 2021

ABSTRACTMany salmonids have a male heterogametic (XX/XY) sex determination system, and they are supposed to have a conserved master sex determining gene (sdY), that interacts at the protein level with Foxl2 leading to the blockage of the synergistic induction of Foxl2 and Nr5a1 of the cyp19a1a promoter. However, this hypothesis of a conserved master sex determining role of sdY in salmonids is still challenged by a few exceptions, one of them being the presence of some naturally occurring “apparent” XY Chinook salmon females. Here we show that XY Chinook salmon females have a sdY gene (sdY-N183), which has one missense mutation leading to a substitution of a conserved isoleucine to an asparagine (SdY I183N). In contrast, Chinook salmon males have both a non-mutated sdY-I183 gene and the missense mutation sdY-N183 gene. The 3D model of SdY-N183 predicts that the I183N hydrophobic to hydrophilic amino acid change leads to a local modification of the β-sandwich structure of SdY. Using in vitro cell transfection assays we found that SdY-N183, like SdY-I183, is preferentially localized in the cytoplasm. However, compared to SdY-I183, SdY-N183 is more prone to degradation, its nuclear translocation by Foxl2 is reduced and SdY-N183 is unable to significantly repress the synergistic Foxl2/Nr5a1 induction of the cyp19a1a promoter. Altogether our results suggest that the sdY-N183 gene of XY Chinook females is a non-functional gene and that SdY-N183 is no longer able to promote testicular differentiation by impairing the synthesis of estrogens in the early differentiating gonads of wild Chinook salmon XY females.
Crystal structure of copper perchlorophthalocyanine analysed by 3D electron diffraction
Authors: Gorelik Tatiana E. , Habermehl Stefan , Shubin Aleksandr A. , Gruene Tim , Yoshida Kaname , Oleynikov Peter , Kaiser Ute , Schmidt Martin U. 
Published: July 2021

Copper perchlorophthalocyanine (CuPcCl16, CuC32N8Cl16, Pigment Green 7) is one of the commercially most important green pigments. The compound is a nanocrystalline fully insoluble powder. Its crystal structure was first addressed by electron diffraction in 1972 [Uyeda et al. (1972). J. Appl. Phys.
43, 5181–5189]. Despite the commercial importance of the compound, the crystal structure remained undetermined until now. Using a special vacuum sublimation technique, micron-sized crystals could be obtained. Three-dimensional electron diffraction (3D ED) data were collected in two ways: (i) in static geometry using a combined stage-tilt/beam-tilt collection scheme and (ii) in continuous rotation mode. Both types of data allowed the crystal structure to be solved by direct methods. The structure was refined kinematically with anisotropic displacement parameters for all atoms. Due to the pronounced crystal mosaicity, a dynamic refinement was not feasible. The unit-cell parameters were verified by Rietveld refinement from powder X-ray diffraction data. The crystal structure was validated by many-body dispersion density functional theory (DFT) calculations. CuPcCl16 crystallizes in the space group C2/m (Z = 2), with the molecules arranged in layers. The structure agrees with that proposed in 1972.
Resilience of Islamic cryptocurrency markets to Covid-19 shocks and the Federal Reserve policy
Authors: Mnif Emna , Jarboui Anis 
Published: July 2021

PurposeAfter the COVID-19 outbreak, the Federal Reserve has undertaken several monetary policies to alleviate the pandemic consequences on the stock markets leading to a misunderstanding on the cryptocurrency market response. This paper aims to evaluate the effects of the Federal Reserve monetary policy on the Islamic and conventional cryptocurrency dynamics during the COVID-19 pandemic. We, specifically, examine the associate bubbles and feedbacks effects.Design/methodology/approachThis paper developed a novel methodology that detects market bubbles using the statistical indicators defined by Psychological (PSY) tests. It also investigated the effect of the Federal Open Market Committee (FOMC) announcements on conventional and Islamic cryptocurrencies compatible with Islamic laws “Shari’ah” by using the event-driven regression.FindingsThe empirical results show that the FOMC announcements have a positive significant effect after one day of the event and a negative effect before two days of the announcement on the conventional cryptocurrency markets. However, the reaction of Islamic cryptocurrencies to these events is not significant except for Hello Gold after one day of the announcement. Besides, the Hello Gold and X8X cryptocurrencies present no bubbles during this period. However, Bitcoin and Ethereum markets have short-lived bubbles.Research limitations/implicationsThe main contribution of this study is the investigation of the response and vulnerability to pandemic shocks of a new category of cryptocurrencies backed by tangible assets. This work has practical implications as it provides new insights into trading opportunities and market reactions.Originality/valueTo our knowledge, this work is the first study that compares the response of Islamic and conventional cryptocurrency markets to FOMC announcements during the COVID-19 pandemic and examines the presence of bubbles in these markets. Besides, the originality of this work is derived from the novelty of the data employed and the method used (PSY tests) in this study.
The moderation effect of work engagement on entrepreneurial attitude and organizational commitment: evidence from Thailand’s entry-level employees during the COVID-19 pandemic
Authors: Boonsiritomachai Waranpong , Sud-On Ploy 
Published: July 2021

PurposePast research has convincingly shown that the more entrepreneurial employees are, the more likely they are to leave the organization and start their own enterprise. Therefore, the purpose of this paper is to understand how entrepreneurial employees can be retained in organizations by examining the relationships between work engagement, organizational commitment and entrepreneurial attitude among entry-level employees.Design/methodology/approachCovariance-based structural equation modeling is used to test the hypothesized relationships by using a survey of 416 employees.FindingsThe results of the study indicate that an entrepreneurial attitude significantly and positively contributes to work engagement, although it can divert employee attention regarding the level of commitment. Interestingly, the study findings show that employees will commit to an organization if they have engaged with the company, even if they have a highly entrepreneurial attitude.Practical implicationsThis study’s findings are beneficial in understanding the concept of an entrepreneurial attitude and its impact on an organization. For firms to concurrently promote entrepreneurial characteristics and employee commitment, policy and staff practices must ensure the appropriate level of work engagement.Originality/valueThis study proposes new distal antecedent and consequence constructs of the engagement theory related to entrepreneurial attitude orientation and establishes the important link between these constructs with work engagement. The findings originally point to the role of work engagement in maintaining entrepreneurial employees and its effect on organizational commitment.
A half-bridge IGBT drive and protection circuit in dielectric barrier discharge power supply
Authors: Wang Xingquan , Lu Xiuyuan , Chen Wei , Wang Fengpeng , Huang Jun , Liu Lingli , Li Mengchao , Lin Kui 
Published: July 2021

Purpose
This paper aims to improve the general circuit of driving and protection based on insulated gate bipolar transistor (IGBT) in dielectric barrier discharge power supply by designing a novel half-bridge inverter circuit with discrete components.

Design/methodology/approach
With one SG3524 chip, the structure based on discrete components is used to design the IGBT drive circuit. The driving waveform is isolated and sent out by photo-coupler 6N137. The protection circuit is realized by Hall sensor directly detecting the main circuit current, supplemented by a few components, including diodes, resistors, capacitors and triodes. It improves the reliability of the protection circuit.

Findings
In the driving circuit, the phase difference of signals from two channels are 180°. Moreover, when the duty cycle is set at 40%, it can ensure sufficient pulse width modulation response time. In the protection circuit, when over-current occurs, an intermittent output signal is automatically sent out. Furthermore, the over-current response time can be controlled independently. The peak voltage can be adjusted continuously from 0 to 30 kV with its frequency from 8 to 25 kHz and the power output up to 150 W.

Originality/value
The novel circuit of driving and protection makes not only its structure simpler and easier to be realized but also key parameters, such as frequency, the duty cycle and the driving voltage, continuously adjustable. Moreover, the power supply is suitable for other discharges such as corona discharge and jet discharge.
Longitudinal associations between soft skills, education and labour market outcomes: evidence from a survey of young Australians
Authors: Forrest Cameron J. , Swanton Tasman 
Published: July 2021

PurposeThe purpose of this paper is to develop a measure of soft skills suitable for use in a large survey of Australian adolescents.Design/methodology/approachA survey was conducted with N = 4,704 Australians aged 15–19 over 2 years. Principal components analysis was performed on 14 self-report items, followed by generalised linear mixed modelling predicting education- and employment-related outcomes.FindingsSelf-reported problem solving, creativity, teamwork and verbal communication were alternately associated with later high school performance, hourly wage and employment status. These effects persisted when controlling for demographics and prior academic achievement.Originality/valueExisting measures have been limited by their length or focus on specific skills or industries. The presented measures are short, domain-general, measure multiple skills simultaneously, and are suitable for a wide range of research contexts.
Factors influencing Bumiputera contractors' acceptance of the contractor's all risk takāful product
Authors: Ibrahim Mohd Azizi , Mat Nor Alias , Raja Hisham Raja Rizal Iskandar 
Published: July 2021

PurposeThis research aims to investigate the factors that can influence Bumiputera contractors’ acceptance of the Contractor’s All Risks (CAR) takāful (Islamic insurance) product using the theory of reasoned action (TRA).Design/methodology/approachUsing the method of quantitative surveys, 414 questionnaires were collected from targeted Bumiputera contractors in Malaysia (grade G1 to grade G7), except in Sabah and Sarawak, that are registered with the Construction Industry Development Board (CIDB) and are also members of Persatuan Kontraktor Melayu Malaysia (PKMM). The data was analysed using the partial least squares structural equation modeling (PLS-SEM) technique.FindingsThe findings from the PLS-SEM analysis show that attitude, subjective norm, religiosity and awareness have a positive relationship with Bumiputera contractors’ acceptance of the CAR takāful product. Religiosity appeared to be the most significant factor influencing Bumiputera contractors’ acceptance of the CAR takāful product.Research limitations/implicationsThe respondents in this study only comprised Bumiputera contractors in Peninsular Malaysia, excluding those from Sabah and Sarawak. Therefore, it is not possible to generalise the findings to a broader population.Practical implicationsTakāful operators and their sales and marketing departments need to act proactively in promoting the benefits of investing in CAR takāful that follows Sharīʾah (Islamic law) rules and principles. They should create mechanisms to market CAR takāful better, thus accelerating its acceptance rate among contractors.Originality/valueThe paper uses the proposed extended TRA model, which includes the variables of religiosity and awareness in the TRA model. These variables were successfully integrated in the model, and the findings show that they have significantly contributed to the acceptance of the CAR takāful product among Bumiputera contractors.
Examining the influence of mindfulness on organizational role stress (ORS): a monitor acceptance theory perspective
Authors: Warrier Uma , Foropon Cyril , Chehimi Melinda 
Published: July 2021

PurposeThe purpose of this paper is to examine the influence of mindfulness on organizational role stress (ORS) based on the Monitor Acceptance Theory (MAT) perspective.Design/methodology/approachThis study is based on a cross-sectional data analysis collected from 137 employees working at an Indian IT organization located in Bangalore (India). ORS and MAAS scales have been used for measuring ORS and mindfulness, respectively.FindingsOverall, the study findings have indicated a negative relationship (r = −0.588) between mindfulness (M) and ORS. First, both personal inadequacy (PI) and self-role distance (SRD) are found to be predominantly impacted by M, whereas both role erosion (RE) and role overload (RO) appear to be less affected by mindfulness. Second, SRD appears to be the highest ORS sub-dimension among IT employees. Third, building on the extant literature, it can be inferred that “no one size fits all”, ORS is both organization and context specific.Originality/valueThis study pioneers to establish empirical evidence between M and ORS. Training employees on M can help in effectively handling ORS.
Environmentally specific transformational leadership and green product development performance: the role of a green HRM system
Authors: Yan Jiaqi , Hu Wenan 
Published: July 2021

PurposeGreen product development is a pivotal way to achieve environmental sustainability. The purpose of this paper is to theorize and empirically test how environmentally specific leadership enhances the green product development performance from the perspective of the HRM system. In this regard, the authors investigate the mediating role of the strength of the HRM system to change with regard to the relationship between environmentally specific leadership and green product development performance. For a substantial explanation of the boundary condition, the authors investigate the moderating role of the green HRM on the relationship between environmentally specific transformational leadership and the strength of the HRM system.Design/methodology/approachBased on climate strength theory, the authors use the three-wave survey data from 362 top or middle managers in the new energy industry of China. This study uses hierarchical linear regression and bootstrapping method to analyze the mediated moderation effect.FindingsResults confirm a positive effect of environmentally specific leadership and the strength of the HRM system on green product development performance. The authors also found the mediation effect of the strength of the HRM system and the moderation effect of green HRM are all significant.Originality/valueThis study integrates the perspectives of both content-focused HRM and process-focused HRM and demonstrates why leadership and the HRM system could jointly enhance green product development performance in Asia.
Measuring an employer brand: a study towards valid scale development (as a second-order factor of a structural model)
Authors: Shrivastava Navin Kumar , Shukla Arvind Virendranath 
Published: July 2021

Purpose
The study aims to conceptualise, develop and validates a scale to measure an employer brand(ing) (EB) based on the opinion of existing and potential employees.

Design/methodology/approach
A total of 431 student-respondents from B-Schools across India were surveyed in a cross-sectional study using a 70-item scale generated through literature review and expert interviews. Through exploratory factor analysis, six EB dimensions were derived. These were further validated using confirmatory factor analysis on data of 120 employees of the power sector.

Findings
A new 20-item EB scale- “EmBran” covers six dimensions of EB, namely, good human resource (HR) practices, business impression and work conditions, financial compensation, work-life balance, passive culture and standard HR policy. The paper posits EB as a second-order factor determined by six first-order factors.

Practical implications
The EB scale can be used by talent acquisition teams to derive meaningful insights into designing a policy for hiring and attracting young talent. It, thus, makes a significant contribution towards talent management. The scale also provides researchers with a fresh conceptualisation of the concept of the EB.

Originality/value
This study is unique as it considers the opinions of both existing and potential employees. Additionally, dimensions of passive culture and business impression and working conditions, emerged in the study.