bluuebunny/crossref_metadata_2025_split huggingface dataset:
Expression of Platelet Derived Growth Factor a, Its Receptor, and Integrin Subunit Alpha V in Feline Injection-Site Sarcomas
Authors: Ahmed Ishtiaq , Mahmut Sozmen 
Published: April 2021

Abstract
Injection-site sarcomas are considered an important entity in veterinary oncology, especially in cats. The current study investigated the immunohistochemical expression of platelet-derived growth factor A (PDGFA), platelet-derived growth factor receptor-α (PDGFR-α), and integrin alpha subunit v in feline injection-site sarcomas (FISS). A total of 14 paraffin-embedded tissue samples previously diagnosed as soft tissue sarcomas were selected from the departmental archive and sectioned at 5µm using a microtome. Tissue sections were stained with Hematoxylin & Eosin, Alcian blue-Periodic Acid Schiff (AB-PAS), Masson’s Trichrome, and immunohistochemically for PDGFA, PDGR-α, and integrin alpha v. The majority of the tumors were fibrosarcomas (n=13/14), except one case of myxoid liposarcoma. The tumors occurred at sites commonly used for injections or vaccine administration. Histologically, tumors were characterized by peripheral infiltration of mononuclear cells mainly lymphocytes, variable sized central necrotic areas, and many multinucleated tumor giant cells. AB-PAS staining revealed the presence of myxoid or mucinous areas in (8/14) tumors while trichrome staining demonstrated a variable amount of collagenous stroma in 12/14 tumors. Vimentin immunoreactivity was observed in all the tumors while smooth muscle actin and muscle actin staining was noticed in four and two cases, respectively. PDGFA and PDGFR-α immunoexpression was observed in all 14 cases while integrin alpha v in 13/14 cases. The results of the current study indicate that fibrosarcoma is the major morphologic phenotype of FISS. The PDGFA, its receptor, and integrin alpha v immunoexpression are increased in these tumors in cats which elucidates their role in the pathogenesis of FISS.
Genetic Analysis and Distribution of Porcine Parvoviruses Detected in the Organs of Wild Boars in Serbia
Authors: Jakov Nišavić , Nenad Milić , Andrea Radalj , Dejan Krnjaić , Dragan Milićević , Aleksandra Knežević , Marina Radojičić , Sonja Obrenović , Milivoje Ćosić , Bojana Tešović , Damir Benković , Aleksandar Živulj 
Published: April 2021

Abstract
Porcine parvoviruses (PPVs) are diverse and persistently evolving viruses found in domestic pigs and wild boars. Porcine parvovirus 1 (PPV1) causes reproductive problems in adult animals, although the veterinary relevance of PPV2, PPV3, and PPV4 has not been clarified. The detection and sequence analysis of PPVs circulating in wild boar populations in Serbia was performed to determine their phylogenetic relationships and prevalence in 122 organ samples collected during 2018. The DNA of PPV1, PPV2, and PPV3 was detected in 56.6% of the examined samples, whilst PPV4 was not identified. Overall, PPV3 was the most prevalent in 69.6% of the positive samples, followed by PPV1 in 63.8%, and PPV2 in 21.7% samples. Single infections were more common, although concurrent infections were confirmed in 34.8% samples for two, and 10.1% samples for three viruses. Sequence analysis of wild boar PPV1 showed no significant nucleotide differences from domestic pig PPV1 strains detected in Europe and the USA, however separate clustering from strains from China and the NADL-2 strain was demonstrated. Examination of the selected PPV2 sequences might suggest a certain geographical distribution of genetically diverse PPV2 strains considering high similarities to the strains from neighboring countries, and variability in comparison with other reported PPV2 sequences from different parts of the world. Wild boar PPV3 sequences clustered separately from most of the strains detected in wild boars, as well as the original porcine hokovirus strain. It is further noted that genetically different PPV3 strains circulate amongst Serbian domestic pigs and wild boars.
Rapid Identification for Serotyping of African Swine Fever Virus Based on the Short Fragment of the EP402R Gene Encoding for CD2-Like Protein
Authors: Thanh Tran Ha Thi , Duc Truong Anh , Viet Ly Duc , Van Hoang Tuan , Thi Nguyen Chinh , Thi Chu Nhu , Thi Nguyen Huyen , Vu Dang Hoang 
Published: April 2021

Abstract
The first confirmed case of African swine fever (ASF) in Vietnam was officially reported in February 2019. To date, the ASF virus (ASFV) has been detected in all 63 cities/provinces in Vietnam. In order to get a better understanding of the potential role of the EP402R gene in a grouping of ASFV serotypes, thirty ASFV sequences of EP402R genes (accession numbers: MN711757-86) from North Central Coast of Vietnam and 68 well-known references of serotype groups from previous studies were further analyzed. Interestingly, we found that a short fragment of 90 nucleotides was very typical for 8 serological groups of ASFVs. A primer set was designed to amplicon the short fragment of 90 nucleotides using the Primer3 program to establish a simplified method for the serotyping of ASFV. Our results indicated that phylogenetic analysis of the short fragment (90 nucleotides) of the EP402R gene is a very specific and useful method for ASFV serotyping when compared to the previous method using a long fragment (816 nucleotides) of this gene and well-known serotype references based on haemadsorption inhibition (HAI) assay.
First Confirmed Clinical Case of Anaplasma platys in a Dog in Serbia
Authors: Anja Ilić Božović , Milena Radaković , Kristina Spariosu , Phyllis Tyrrell , Ramaswamy Chandrashekar , Dušan Mišić , Milica Kovačević Filipović 
Published: April 2021

Abstract
In September 2018, a four-month-old dog with fever and petechial bleeding came to the internal clinic at the Faculty of Veterinary Medicine University of Belgrade. On hematology analysis, thrombocytopenia and mild anemia were observed. Examination of the blood smear revealed platelet inclusions. The commercial serology test was positive for Anaplasma spp. The dog was treated with doxycycline for 14 days, and after 48 hours from the beginning of the treatment, the symptoms subsided. PCR analysis and sequencing confirmed infection with A. platys.
The Antioxidant Analysis of Selected Types of Climbing Plants with Therapeutic Effect
Authors: Hrúzová M. , Mňahončáková E. , Šimková J. 
Published: April 2021

Abstract
Climbing plants with therapeutic effects are relatively little studied group of medicinal species. In the collection of climbing species planted in 2019 in the Botanical Garden of the Slovak University of Agriculture (SUA) in Nitra, we observed the growth and potential food, horticulture and healing use. For the antioxidant analysis, 7 species from 152 plants of 13 genera and 22 species were selected. The highest antiradical activity was recorded in the species Vigna sinensis, where reached average measured values achieved up to 73.79%. However, the related species Vigna unguiculata achieved only 26% of antioxidant activity. Of the fruits, the highest activity was Momordica charantia in immature state (73%). Other fruits showed very low antiradical activity
Light Two-Wheeled Electric Vehicle Energy Balance Investigation Using Chassis Dynamometer
Authors: Racewicz Szymon , Kazimierczuk Paweł 
Published: April 2021

Abstract
The article presents the methodology for light two-wheeled electric vehicle energy balance investigation using MAHA LPS 3000 chassis dynamometer. For this purpose, the laboratory tests, as well as the road tests, have been performed on the self-constructed light two-wheeled electric vehicle equipped with the 3 kW BLDC motor and the 100.8 V battery pack. The road test data have been used to set up the dynamometer parameters in order to simulate the real road drive taking into account, among others, the rolling resistances and the air drag coefficient. The overall energy consumption for the laboratory tests and the road conditions’ tests have been then compared for different vehicle speeds. In addition, based on the power and the torque characteristics measured on the MAHA chassis dynamometer, the efficiency of the vehicle electric drive system and the efficiency of the battery charging process have been calculated.
Technical, Biological and Molecular Aspects of Somatic Cell Nuclear Transfer – A Review
Authors: Mrowiec Patrycja , Bugno-Poniewierska Monika 
Published: April 2021

Abstract
Since the announcement of the birth of the first cloned mammal in 1997, Dolly the sheep, 24 animal species including laboratory, farm, and wild animals have been cloned. The technique for somatic cloning involves transfer of the donor nucleus of a somatic cell into an enucleated oocyte at the metaphase II (MII) stage for the generation of a new individual, genetically identical to the somatic cell donor. There is increasing interest in animal cloning for different purposes such as rescue of endangered animals, replication of superior farm animals, production of genetically engineered animals, creation of biomedical models, and basic research. However, the efficiency of cloning remains relatively low. High abortion, embryonic, and fetal mortality rates are frequently observed. Moreover, aberrant developmental patterns during or after birth are reported. Researchers attribute these abnormal phenotypes mainly to incomplete nuclear remodeling, resulting in incomplete reprogramming. Nevertheless, multiple factors influence the success of each step of the somatic cloning process. Various strategies have been used to improve the efficiency of nuclear transfer and most of the phenotypically normal born clones can survive, grow, and reproduce. This paper will present some technical, biological, and molecular aspects of somatic cloning, along with remarkable achievements and current improvements.
The Importance of Brownfield Regeneration and Improvement
Authors: Romanovska-Grīnberga Kristiāna , Ulme Andra 
Published: April 2021

Abstract
this research aims to explore the historical development, current situation, weaknesses and strengths of brownfield revitalization, as well as threats and possible solutions. Current situation in legislation and real life has been viewed, successful solutions globally and particularly in Latvia have been explored, innovation ideas, problems and recommendations for the improvement of the situations have been researched.
Effect of Water pH on Domestic Machine Washing Performance of Delicate Textiles
Authors: Çakmakçı Nilüfer , Candan Cevza , Arslan Başak İlkiz 
Published: April 2021

Abstract
In order to investigate the effect of washing water pH on textile damage for delicate garments, the study was conducted such that a customer survey was first applied to randomly selected users to analyze domestic washing machine using habits of Turkish consumers. Based on the findings of the survey, the experimental study was designed in two successive phases to investigate the dimensional and mechanical behavior of certain types of delicate textiles against varying washing conditions by taking in particular, water properties, namely pH, hardness, and temperature into account, and accordingly to determine the optimized washing conditions for such textiles. Firstly, tergotometer was employed as a washing machine simulator. Within the light of the results obtained, the experimental work of the second phase of the study was conducted, which involved a domestic washing machine as a real-life scenario. All of the results, including the correlation between the data sets obtained from the tergotometer and domestic washing machine trials, were statistically analyzed using Minitab 17. The study produced some important findings regarding the effect of washing water pH on delicate textiles, in addition to an algorithm for improving the present washing program, minimizing textile damage for mainly wool and silk garments.
Some variants of Ćirić’s multi-valued contraction principle
Authors: Alecsa Cristian Daniel , Petruşel Adrian 
Published: April 2021

Abstract
In this article, a study of the fixed point problem for Ćirić type multi-valued operators is presented. More precisely, some variants ofĆirić’s contraction principle for multi-valued operators, as well as a strict fixed point principle forĆirić type multi-valued will be given.
Correlation between clinical tests for gait and stability using biomechanical variables in the gait of institutionalized elderly subjects
Authors: Palma Felipe H. , Rodríguez Sebastián Cisternas , Buton Francisco Vargas , Nieva Marcela Olmos , Redenz Günther , Guzmán-Venegas Rodrigo 
Published: April 2021

Abstract

Study aim: This study aims to identify biomechanical gait variables explaining clinical test results in institutionalized elderly people.

Material and methods: Twenty-nine elderly (82.0 ± 6.3 years) residents in a nursing home were assessed. They were able to walk 10 meters without walking aids. First, the spontaneous gait was assessed using inertial measurement units in a 10-meter long corridor. Fifteen biomechanical gait variables were analyzed. Then, three clinical tests usually used in elderly subjects were applied: the Timed Up and Go (TUG) test, the Tinetti Scale and the Sit to Stand (STS) test. A correlation matrix using Pearson’s correlation coefficient between clinical and biomechanical variables was performed, obtaining a total of 45 potential correlations. A stepwise multiple linear regression analysis was then performed to determine the influence of each variable.

Results: TUG, Tinetti and STS were significantly correlated with similar biomechanical variables, including temporal, temporo-spatial and kinematic variables. Adults over 80 years old and women showed stronger correlations. Single support and ankle angle at takeoff were the two most important variables in stepwise regression analysis.

Conclusions: In institutionalized elderly subjects, clinical variables for gait and postural stability are correlated with the biomechanical gait variables, especially in women and adults aged over 80 years.
Law as a Language, Law as an Art: Reflections on James Boyd White's Keep Law Alive
Authors: Jefferson Powell H. 
Published: April 2021

Abstract
Keep Law Alive, the latest book by law and literature scholar James Boyd White, is an important apologia for the traditional understanding and practice of law in the United States. Law, White argues, has served as a language in a sense closely parallel to what we mean by referring to English or Spanish as a language: law provides those fluent in it with the tools to describe the social world and to imagine its transformation, but without scripting what the speaker must say. White also envisions law as an art that evokes imagination, emotion and personal judgment, as well as the mind, and that is fundamentally oriented toward the realization of justice. Intellectual, social and political changes, however, threaten to displace law as a language and art with a view of law as an essentially empty rhetoric that cloaks the use of abstract and impersonal reasoning often borrowed from other disciplines. The survival of law depends on the willingness of those who speak it to continue its practice as an art that serves a humane vision of political life.
The Principle of the Separation of Powers: the Ontological Presumption of an Ideologeme
Authors: Mesonis Gediminas 
Published: April 2021

Abstract
The theoretical materiality of the principle of the separation of powers is beyond doubt. This principle is inevitable in discourse on the constitutional framework of the state, democracy and the rule of law, and it has its own form of expression in positive law. Although the relevance of the principle of the separation of powers in social discourse creates the illusion of the conceivability of its content, the ontological questions concerning this principle remain largely vague. This can be explained by considering two aspects. First, as established in scientific doctrines and constitutional forms of expression, the principle of the separation of powers has become a social and legal ideologeme; it approximates an axiom which is no longer substantiated anew. Second, discourse concerning ontology is always complicated, since it calls to question the essence itself. It is complicated not only because it requires a particular intellectual effort and academic courage, but also because the outcome of such discourse is unpredictable and can lead either to the ideologeme being confirmed to be true or being unexpectedly revised, or perhaps can even lead to the demise of what has so far been self-evident, unquestionable, obvious, universally known, etc. This article analyses the ontological essence of the principle of the separation of powers – an approach towards the human being, whereby meaning is given to the consequent system of causal relationships within the whole theory. Discourse in this article takes ontological issues as its object of inquiry: why did we decide to separate powers and how many of these separated powers are there?
Modeling the Patterns of Civil Confiscation: Balancing Effectiveness, Proportionality and the Right to Be Presumed Innocent
Authors: Bikelis Skirmantas 
Published: April 2021

Abstract
This article elaborates on recent developments in modelling the advanced measure for prevention of organized and serious criminality and corruption – civil confiscation. It distinguishes and discusses the safeguards in civil confiscation patterns that are supposed to ensure the balance between the effectiveness and proportionality of the recovery of the proceeds of crime. Based on different sets of the distinguished safeguards, the article abstracts the variety of civil confiscation patterns in European national jurisdictions into three models and discusses the advantages and the risks the regulation based on these models may pose. The analysis is supplemented with the assessments made by the European Court of Human Rights in the cases related to civil confiscation regulation and insights of the practitioners who participated in the legislative proceedings on the draft of the Lithuanian law on civil confiscation. The article concludes with the thesis that some patterns of the civil confiscation may pose serious risks of disproportional or erroneous decisions to recover property and abuse of civil confiscation proceedings.
Case report of a successful pregnancy in a cystic fibrosis patient with the c.1521_1523delCTT/c.3718-2477C>t genotypes
Authors: Spasova VL , Koleva LI , Toncheva DI , Karamisheva VI 
Published: April 2021

Abstract
The aim of this case report was to show the consequences of pregnancy in a cystic fibrosis (CF) patient with a rare mutation. We present a case of a patient with CF, pregnant for the second time, who gave birth to a healthy child. Her mutation status revealed the presence of relatively rare mutation c.3718-2477C>T that is associated with a milder phenotype of the disease. During pregnancy, her vital signs were within normal limits. She had no exacerbations after the third gestational month. Cystic fibrosis is the most common genetic disorder among Caucasians. Over the last few decades, the survival rate and the lifespan of patients with CF have increased progressively. This is why more affected women are choosing to become pregnant. Predictive factors for the pregnancy outcome are basal pulmonary function [measured by forced expiratory volume/1 second (FEV1)], nutritional status [measured by body mass index (BMI)], diabetes and bacterial colonization. The report of our case emphasizes the need for establishing the exact mutations in CF patients who plan to become pregnant in order to predict the possible outcomes of this specific period of life. Moreover, genetic counseling is strongly recommended for the right understanding of the pregnancy risks in such cases.
Osteopetrorickets in an infant with coexistent congenital cytomegalovirus infection
Authors: Katsafiloudi M , Gombakis N , Hatzipantelis E , Tragiannidis A 
Published: April 2021

Abstract
Osteopetrosis refers to a group of rare hereditary disorders characterized by generalized skeletal densification due to limited bone resorption by osteoclasts. The infantile autosomal recessive form represents the most malignant one with onset early in infancy and life expectancy less than 1-2 years without therapy. Frequently, osteopetrosis is complicated by rickets, a condition called osteopetrorickets. Currently, bone marrow transplantation remains the only treatment option. We present a case of infantile autosomal recessive osteopetrosis complicated by rickets in a 2 and a half-month-old female infant with coexistent congenital cytomegalovirus (CMV) infection, successfully treated by hematopoietic stem cell transplantation (HSCT). Diagnostic procedure and differential diagnosis are discussed along with a short review of the literature. Diagnosis of osteopetrosis requires high clinical suspicion, which is enhanced by radiology and confirmed by bone biopsy and molecular analysis. Our patient has been successfully treated by HSCT and has remained in a good general condition thereafter.
Array-comparative genomic hybridization results in clinically affected cases with apparently balanced chromosomal rearrangements
Authors: Satkin NB , Karaman B , Ergin S , Kayserili H , Kalelioglu IH , Has R , Yuksel A , Basaran S 
Published: April 2021

Abstract
Carriers of apparently balanced chromosomal rearrangements (ABCRs) have a 2-3-fold higher risk of carrying an abnormal phenotype, when compared to the average population. Apparently balanced chromosomal rearrangements can be imbalanced at the submicroscopic level, and changes in the gene structure, formation of a new chimeric gene, gain or loss of function of the genes and altered imprinting pattern may also affect the phenotype. Chromosomal microarray (CMA) is an efficient tool to detect submicroscopic imbalances at the breakpoints as well as in the whole genome. We aimed to determine the effectiveness of array-comparative genomic hybridization (aCGH) application in phenotypically affected cases with ABCRs at a single center from Turkey. Thirty-four affected cases (13 prenatal, 21 postnatal) carrying ABCRs were investigated with CMA. In postnatal series, ABCRs were familial in 7 and de novo in 14 cases. Seven de novo cases were imbalanced (in postnatal series 33.3% and in de novo cases 50.0%). Out of 13 prenatal cases, five were familial and eight were de novo in origin and two de novo cases were imbalanced (in 15.4% prenatal series and in 25.0% de novo cases). No cryptic imbalance was observed in familial cases. The anomaly rates with array studies ranged between 14.3-25.0% in familial and between 20.0-57.5% in de novo cases of postnatal series in the literature. Studies focused on prenatal ABCR cases with abnormal ultrasound findings are limited and no submicroscopic imbalance was reported in the cohorts. When de novo postnatal or prenatal results were combined, the percentage of abnormalities detected by CMA was 40.9%. Taking this contribution into consideration, all ABCRs should be investigated by CMA even if the fetal ultrasound findings are normal.