bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Drosophila Toolkit for Imaging of HA-tagged Proteins Unveiled a Block in Autophagy Flux in the Last Instar Larval Fat Body
Authors: Murakawa Tadayoshi , Nakamura Tsuyoshi , Kawaguchi Kohei , Murayama Futoshi , Ning Zhao , Stasevich Timothy J , Kimura Hiroshi , Fujita Naonobu 
Published: May 2021

AbstractFor in vivo functional analysis of a protein of interest (POI), multiple transgenic strains with POI harboring different tags are needed but generation of these strains is still labor-intensive work. To overcome this, we developed a versatile Drosophila toolkit with a genetically encoded single-chain variable fragment for the HA epitope tag: “HA Frankenbody”. This system allows various analyses of HA-tagged POI in live tissues by simply crossing an HA Frankenbody fly with an HA-tagged POI fly. Strikingly, the GFP-mCherry tandem fluorescent-tagged HA Frankenbody revealed a block in autophagic flux and an accumulation of enlarged autolysosomes in the last instar larval and prepupal fat body. Autophagy was dispensable for the swelling of lysosomes, indicating that lysosomal activity is downregulated at this stage. Furthermore, forced activation of lysosomes by fat body-targeted overexpression of Mitf, the single MiTF/TFE family gene in Drosophila, suppressed the lysosomal swelling and resulted in pupal lethality. Collectively, we propose that downregulated lysosomal function in the fat body plays a role in the metamorphosis of Drosophila.
Distinct Origins of Two-order Hierarchical Cognitive Abilities in Human Adults
Authors: Jiang Shaohan , Sun Fanru , Yuan Peijun , Jiang Yi , Wan Xiaohong 
Published: May 2021

AbstractHuman cognitive abilities are considerably diverse from basic perceptions to complex social behaviors. All human cognitive functions are principally categorized into a two-order hierarchy. Almost all of the first-order cognitive abilities investigated in behavioral genetics have been found to be dominantly heritable. However, the origins of the human second-order cognitive abilities in metacognition and mentalizing so far remain unclear. We here systematically compared the origins of the first-order and second-order cognitive abilities involved in the metacognition and mentalizing tasks using the classical twin paradigm on human adults. Our results demonstrated a double dissociation of the genetic and environmental contributions to the first-order and second-order cognitive abilities. All the first-order cognitive abilities involved in the metacognition and mentalizing tasks were dominantly heritable. In contrast, the shared environmental effects, rather than the genetic effects, had dominant contributions to the second-order cognitive abilities of metacognition and mentalizing in human adults. Hence, our findings suggest that human adults’ monitoring sensitivities in metacognition and mentalizing are profoundly sculpted by their social or cultural experiences, but less preconditioned by their biological nature.
Big Five personality traits impact on entrepreneurial intention: the mediating role of entrepreneurial alertness
Authors: Awwad Mohammad Suleiman , Al-Aseer Rana Mohammad Najati 
Published: May 2021

Purpose
The purpose of this study is to investigate the impact of the Big Five personality traits on the entrepreneurial intentions of undergrad university students in Jordan. It further investigates the mediating role of entrepreneurial alertness.

Design/methodology/approach
A quantitative survey method was conducted with a convenience sample of Jordanian university students. A total of 323 valid questionnaires were received and analyzed. A structural equation modeling with partial least square (PLS) is used to analyze data.

Findings
Results revealed that conscientiousness, openness and alertness were associated with entrepreneurial intention. Extraversion and openness were associated with alertness, while agreeableness and neuroticism were unrelated to either outcome. Finally, alertness mediates the relationship between extraversion and openness with entrepreneurial intention.

Originality/value
There is a lack of previous studies investigating the relationship between the Big Five personality traits and students’ entrepreneurial intentions in Jordan, particularly the role of mediating variables in this relationship. This study is considered the first one that examined the mediating role of entrepreneurial alertness in the relationship between personality traits and entrepreneurial intentions.
Rayleigh wave propagation at the boundary surface of dry sandy ($SiO_2$) thermoelastic solids
Authors: Gupta Shishir , Dwivedi Rishi , Smita Smita , Dutta Rachaita 
Published: May 2021

Purpose
The purpose of study to this article is to analyze the Rayleigh wave propagation in an isotropic dry sandy thermoelastic half-space. Various wave characteristics, i.e wave velocity, penetration depth and temperature have been derived and represented graphically. The generalized secular equation and classical dispersion equation of Rayleigh wave is obtained in a compact form.

Design/methodology/approach
The present article deals with the propagation of Rayleigh surface wave in a homogeneous, dry sandy thermoelastic half-space. The dispersion equation for the proposed model is derived in closed form and computed analytically. The velocity of Rayleigh surface wave is discussed through graphs. Phase velocity and penetration depth of generated quasi P, quasi SH wave, and thermal mode wave is computed mathematically and analyzed graphically. To illustrate the analytical developments, some particular cases are deliberated, which agrees with the classical equation of Rayleigh waves.

Findings
The dispersion equation of Rayleigh waves in the presence of thermal conductivity for a dry sandy thermoelastic medium has been derived. The dry sandiness parameter plays an effective role in thermoelastic media, especially with respect to the reference temperature for η = 0.6,0.8,1. The significant difference in η changes a lot in thermal parameters that are obvious from graphs. The penetration depth and phase velocity for generated quasi-wave is deduced due to the propagation of Rayleigh wave. The generalized secular equation and classical dispersion equation of Rayleigh wave is obtained in a compact form.

Originality/value
Rayleigh surface wave propagation in dry sandy thermoelastic medium has not been attempted so far. In the present investigation, the propagation of Rayleigh waves in dry sandy thermoelastic half-space has been considered. This study will find its applications in the design of surface acoustic wave devices, earthquake engineering structural mechanics and damages in the characterization of materials.
Childhood, disability and vocational training in Franco's Spain during the 1950s and early 1960s
Authors: Del Cura Mercedes , Martínez-Pérez José 
Published: May 2021

PurposeThis paper analyses the strategies designed by Franco´s dictatorship to address the “problem” of children with physical disabilities, focusing on the relevance given to vocational training.Design/methodology/approachThe paper draws mainly on official documents, reports from international organisations, and Spanish experts' papers.FindingsFrancoism turned labour into one of the key pillars of its national project and included vocational training in the different stages of school life. From the mid-1950s, vocational training also became a key factor for the dictatorship's strategy towards disability. Following the recommendations issued by international agencies, Francoism began to adopt different measures towards the rehabilitation of children with disabilities. One of them was the creation, in 1959, of a special unit for adolescents within the National Institute for the Rehabilitation of Invalids. In addition to medical treatment, this unit provided children with education and vocational training.Originality/valueThe value of this paper lies in the fact that the topic it analyses has been little studied. Until now no attention has been given to the special unit for adolescents, despite it being a very interesting example of the medical model of disability and its contradictions. During their stay at the unit children were promised greater autonomy and independence, but their lives also became medicalised and they were forced to collaborate with experts.
Individual investor attention to stock split and the disposition effect
Authors: Kesuma Wendy , Ekaputra Irwan Adi , Chalid Dony Abdul 
Published: May 2021

PurposeThis paper investigates whether individual investors are attentive to stock splits and whether higher split ratios (stronger private information signals) reduce the disposition effect.Design/methodology/approachThis study employs stock split events and transaction data in the Indonesia Stock Exchange (IDX) from January 2004 to December 2017. The authors measure individual investors' attention using buy-initiated trades. To test the effect of split signal on disposition effect, the authors regress individual investors' sell-initiated trades on past stock returns.FindingsUnlike Birru (2015), the authors find that individual investors are attentive to stock splits, especially when stock split ratios are high. In turn, stock splits tend to weaken the disposition effect. The higher the stock split ratios, the weaker the disposition effect.Research limitations/implicationsThis study has a limitation in that the authors exclude all stock splits with dividend events around the split date. These stock splits cover 37% of all splits in Indonesia.Practical implicationsPractically, individual investors should look for stock-related information to reduce disposition bias.Originality/valueTo the best of authors’ knowledge, this study is the first to test individual investors' attention on stock splits based on their buy-initiated trades. This study is also the first to test the impact of stock split ratios on the disposition effect reduction. This study's findings enrich the scant literature on individual investors' attention and how to reduce their disposition effect bias.
Tips for difficult to diagnose hypomelanotic melanomas on reflectance confocal microscopy
Authors: Pizzichetta Maria Antonietta , Canzonieri Vincenzo , Rubegni Pietro , Corneli Paola , Puglisi Fabio , Zalaudek Iris , Cinotti Elisa 
Published: May 2021

AbstractAmelanotic/hypomelanotic melanoma (AHM) represents a clinical diagnostic challenge. Dermoscopy improves AHM diagnosis thanks to visualization of little pigment and vascular pattern. Reflectance confocal microscopy (RCM) increases further the diagnostic accuracy of AHM but few and small studies have described in detail RCM features of AHM. We evaluated dermoscopic and RCM features of nine cases of difficult to diagnose hypomelanotic melanomas (HMs) to find clues for their diagnosis. The RCM score was suggestive of melanoma in all cases. The major criteria of nonedged papillae and/or cytological atypia at the dermo‐epidermal junction were seen in all cases. Among the minor criteria, roundish pagetoid cells, including hyporeflective pagetoid cells, were found in four out of nine lesions. Dermoscopically, four out of nine HMs did not show prevalent suspicious criteria while revealing suspicious RCM features that were visible only after careful RCM examination by zooming mosaic images. RCM can improve HM diagnostic accuracy but only after extensive evaluation of images. Atypical cells were less reflective and the architectural irregularity was less visible than in pigmented melanoma and zooming was needed to identify both features.
The role of entitlement, social dominance orientation, and right‐wing authoritarianism in the prediction of homonegativity for heterosexual white men
Authors: Adelman Andrew L. , Awad Germine H. , Bennett Ashley , Nguyen Hien 
Published: May 2021

AbstractWe examined the role of right‐wing authoritarianism (RWA), social dominance orientation (SDO), psychological entitlement, and narcissistic entitlement in the prediction of White men's negative attitudes and behaviors toward lesbian women and gay men. The present study (a) simultaneously examines four predictors (i.e., psychological and narcissistic entitlement, RWA, and SDO) of attitudes toward lesbian women and gay men (ATLG) and rejecting behaviors, (b) examines psychological and narcissistic entitlement as distinct predictors of ATLG and rejecting behaviors, and (c) tests the potential mediating role of ATLG. A path model was tested using the survey data from 153 White, heterosexual, male college students. Findings indicate that only RWA and narcissistic entitlement were significant predictors of ATLG, which is a strong predictor of rejecting behaviors. Furthermore, ATLG mediates the relationship between RWA and rejecting behaviors (d = .35). In other words, higher RWA is associated with higher ATLG, which is associated with more self‐reported rejecting behaviors. Narcissistic entitlement also influences rejecting behaviors through ATLG and both psychological and narcissistic entitlement were found to directly predict gay and lesbian rejecting behaviors.
Digital peer learning for transformative professional agency: The case of homelessness practitioners in Finland
Authors: Sannino Annalisa , Engeström Yrjö , Jokinen Esa 
Published: May 2021

AbstractDigital lifelong learning and more specifically digital peer learning (DPL) can play a major role to foster transformative agency in professions and occupations which are critically positioned for responding to acute societal needs. Yet so far, no published studies seem to have focused on this. This article aims at filling this gap with the help of a study in which online workshops and web forums were created for supporting homelessness practitioners in Finland to share and discuss scattered practical innovations and to generate advanced solutions to problems in their work. By these means, the study also generated data to see if transformative agency takes place among these professionals by means of DPL, how this happens, and with what results for the critical field of homelessness work. This study opens up a new agenda for research and development in lifelong learning in a digital era.
Practitioner notesWhat is already known about this topic

As digital peer learning (DPL) can be largely organized by the learners themselves, it carries significant advantages for lifelong learning and work development: a close link to the field of practice and to clients' and stakeholders' needs, potentially a wide reach of practitioners, little institutional investment, and cost‐effectiveness.
The application of DPL raises a number of challenges we summarize here as the spectator challenge, the challenge of drowning in details, and the discontinuity challenge.
What this paper adds

DPL literature lacks specific contributions on how it can support practitioners to identify and implement concrete solutions to pressing needs in society.
This article shows that DPL may facilitate professional transformative agency in such a way that the two processes can intersect with one another and generate concrete and effective lifelong learning solutions for much needed developments in critical fields such as homelessness work.
Implications for practice and/or policy

Cultivating personally and professionally meaningful conflicts of motives evokes emotional involvement and potentially also learners' curiosity and cognitive engagement, opening an avenue to transcend the spectator stance.
Experience and discursive elaboration of a conflict of motives directs learners to focus on the essential, thus providing an effective means for overcoming the risk of drowning in details. This can be facilitated by offering artifacts, metaphors, or models which may be taken up by practitioners in DPL as support or “second stimuli” to engage in transformative initiatives.
To transcend the discontinuity challenge salient in many DPL processes, it is of particular importance to find ways to embed DPL and engage the learners in long‐term change efforts. Even relatively short online workshops and web forum discussions can gain momentum when efforts are made to establish links between past experiences and the future prospects.
Influence of metal artefact reduction tool on the detection of vertical root fractures involving teeth with intracanal materials in cone beam computed tomography images: A systematic review and meta‐analysis
Authors: Fontenele Rocharles C. , Machado Alessiana H. , de Oliveira Reis Larissa , Freitas Deborah Q. 
Published: May 2021

AbstractBackgroundThe effectiveness of the metal artefact reduction (MAR) tool for the detection of vertical root fracture (VRF) by cone beam computed tomography (CBCT) images is controversial.ObjectiveTo evaluate the effectiveness of the MAR tool on VRF detection involving teeth with intracanal materials in CBCT images.MethodologyIn September 2019, an electronic search was performed in six databases (PubMed, Scopus, Web of Science, Cochrane, Lilacs and Embase). The electronic search was updated in May 2020 through searches in Google Scholar and references of included studies (embracing the electronic and gray literature searches). No language or year restrictions were applied. Independently, two reviewers examined titles, abstracts and full texts according to the eligibility criteria that were as follows: diagnostic studies that evaluated the effectiveness of the MAR tool on the diagnosis of VRF in human teeth (laboratory orin vivostudies) on CBCT images. The risk of bias was evaluated using the Quality Assessment Tool for Diagnostic of Accuracy Studies‐2 (QUADAS‐2). For conducting the meta‐analysis, the values of sensitivity, specificity, diagnostic odds ratio (DOR) and area under the ROC curve (AUC) were obtained, considering the subgroups with and without MAR active. The Grading of Recommendation, Assessment, Development and Evaluation instrument was applied to assess the level of evidence across the studies using GRADEpro software.ResultsA total of 8 laboratory studies were included in both systematic review and meta‐analysis. The values of sensitivity, specificity, DOR and AUC to VRF diagnosis with and without MAR active were, respectively, 0.586 and 0.603; 0.699 and 0.713, 3.037 and 3.767; 0.67 and 0.71. The quality of the evidence suggested low confidence in estimating the outcomes.DiscussionThe MAR tool decreased slightly the diagnostic values of VRF involving teeth with intracanal materials in laboratory studies. However, it is important to highlight that most studies had an uncertain risk of bias.ConclusionsAlthough a low level of evidence and high heterogeneity were observed in the included studies, the meta‐analysis revealed better diagnosis values for VRF detection when the MAR was deactivated when analysing extracted teeth in a laboratory setting.RegistrationPROSPERO—CRD42020145222.FundingThis study was financed in party by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior—Brasil (CAPES).
The detection of long‐lasting memory foot‐and‐mouth disease (FMD) virus serotype O‐specific CD4+ T cells from FMD‐vaccinated cattle by bovine major histocompatibility complex class II tetramer
Authors: Mitoma Shuya , Carr Brigid Veronica , Harvey Yongjie , Moffat Katy , Sekiguchi Satoshi , Charleston Bryan , Norimine Junzo , Seago Julian 
Published: May 2021

AbstractFoot‐and‐mouth disease (FMD) is a highly contagious, economically devastating disease of cloven‐hooved animals. The development of long‐lasting effective FMD vaccines would greatly benefit the global FMD control programme. Deep analysis of adaptive immunity in cattle vaccinated against FMD is technically challenging due to the lack of species‐specific tools. In this study, we aimed to identify CD4+ T‐cell epitopes in the FMD virus (FMDV) capsid and to phenotype the CD4+ T cells that recognize them using bovine major histocompatibility complex (BoLA) class II tetramer. A BoLA class II tetramer based on the DRA/DRB3*020:02 allele and FMDV antigen‐stimulated PBMCs from bovine vaccinates were used to successfully identify four epitopes in the FMDV capsid, three of which have not been previously reported; two epitopes were identified in the structural protein VP1, one in VP3 and one in VP4. Specificity of the three novel epitopes was confirmed by proliferation assay. All epitope‐expanded T‐cell populations produced IFN‐γ in vitro, indicating a long‐lasting Th1 cell phenotype after FMD vaccination. VP3‐specific CD4+ T cells exhibited the highest frequency amongst the identified epitopes, comprising >0·004% of the CD4+ T‐cell population. CD45RO+CCR7+ defined central memory CD4+ T‐cell subpopulations were present in higher frequency in FMDV‐specific CD4+ T‐cell populations from FMD‐vaccinated cattle ex vivo. This indicates an important role in maintaining cell adaptive immunity after FMD vaccination. Notably, FMDV epitope‐loaded tetramers detected the presence of FMDV‐specific CD4+ T cells in bovine PBMC more than four years after vaccination. This work contributes to our understanding of vaccine efficacy.
Nurses’ ehealth literacy and associations with the nursing practice environment
Authors: Kritsotakis George , Andreadaki Eirini , Linardakis Manolis , Manomenidis George , Bellali Thalia , Kostagiolas Petros 
Published: May 2021

AimTo report on ehealth literacy levels in nurses and to explore its associations with the nursing practice environment.BackgroundNurses increasingly use the Internet and associated technologies to seek health‐relevant information and manage their health.IntroductionHigh ehealth literacy is a predictor of better health outcomes in diverse populations but its levels and work‐related determinants have not been adequately explored in direct‐care nurses.MethodsThe sample for this cross‐sectional study consisted of 200 staff nurses and nursing assistants in Greece. Participants reported during February–March 2019 their sociodemographic and work‐related characteristics on a self‐administered questionnaire which included the “electronic Health Literacy Scale”—eHEALS, and the “Practice Environment Scale of the Nursing Work Index”—PES‐NWI. Crude and adjusted logistic regressions were performed.FindingsIn adjusted models, participants that scored higher on the “Collegial nurse–physician relationships” and “Nurse participation in hospital affairs” dimensions of the clinical environment had higher odds of reporting better ehealth literacy. The lowest item score in eHEALS was related to not being able to make health decisions using Internet information.DiscussionNurses’ ehealth literacy was positively associated with some dimensions of the hospital practice environment. Nurses reported higher ehealth literacy scores in comparison to other studies; however, they were not confident in distinguishing reliable health information from Internet sources. This is quite alarming because it can directly impair the ability of nurses to provide relevant and up‐to‐date evidence‐based care.ConclusionThis is the first study to report internationally on the positive associations of a good working environment with nurses’ ehealth literacy levels.Implications for Nursing and Nursing PolicyNursing policy should address the ehealth literary of nurses and integrate it into continuing professional education initiatives. Special focus should be put on nurses’ ambiguity in distinguishing which ehealth information is reliable and can guide nursing practice. This should be combined with efforts to improve the nursing clinical environment and increase nurses’ participation in hospital decisions.