bluuebunny/crossref_metadata_2025_split huggingface dataset:
Method development and Validation for Estimation of Irbesartan and Hydrochlorothiazide in Tablet Dosage form by using RPHPLC
Authors: Swathi Konda , Manasa G Lakshmi , Munemma R , Nikitha B 
Published: March 2021

Abstract
In this study Irbesartan (IRN) and Hydrochlorothiazide (HRE) assay was done by utilizing stability indicating RP-HPLC, in which module was carried the water separation 2695, equipped with a detector and chromatographic separation Phenomenex column C18(250×4.mm,5μm) column was operated as stationary phase and mobile phase was combination of phosphate buffer (0.1M, pH 4.1) and methanol in 60% vol and 40% vol, respectively with isocratic elution type. By using 1.0 ml/min flow rate and effluents were observed at 230 nm. The HRE and IRN were eluted at 2.913 and 2.346 min, respectively. Linearity was 75-225 μg/ml (IRN) and 6.25-18.75 μg/ml (HRE). Limit of detection (LOD) and limit of quantification (LOT) for Irbesartan arel.173 For the purposes of μg/ml, and 3,911 μg/ml, 0.409 μg/ml and 1.364 μg/ml for Hydrochlorothiazide. The procedure was developed to suggest consistency by the subjection of medications to stress conditions like acid, alkaline, peroxide, dry heat and sunlight. Both validated criteria were appropriate. The Granry 150H tablet method can be used.
Design of a solar charging station for electric vehicles in shopping malls
Authors: Peña C , Céspedes M 
Published: March 2021

Abstract
In this article, we present the design, sizing and modeling of a grid-connected solar charging station for recharging electric vehicles in shopping malls. The applied method consists of an analysis of the solar resource available at the location of the shopping mall, as well as the analysis, evaluation and selection of the components of the grid-connected photovoltaic system with the support of simulation software such as PVsyst and Helioscope, as well as analysis, evaluation and selection of the components of the charging points of electric vehicles and finally the economic analysis of the solar charging station in the shopping mall.
Extreme Overirradiance events and their spectral distribution in Lima, Peru
Authors: Zamalloa Jara M A , Berg H , Conde L A , Sevillano-Bendezú M A , Carhuavilca A M , Grieseler R , Töfflinger J A 
Published: March 2021

Abstract
We report on ‘extreme overirradiance’ events measured in Lima, Peru. The highest measured irradiance value was 1543 W/m2 on March 23rd, 2020. The measurements were carried out by four different, independent instruments, all simultaneously recording their maximum values. The spectral distribution of the extreme overirradiance event was also recorded and compared to the spectral distributions of a clear and a cloudy sky. The extreme overirradiance phenomenon demonstrated an irradiance enhancement in the entire measured spectrum, but predominantly in the visible and the near infrared region of the spectrum (450 nm to 1100 nm). This spectral enhancement leads to a redshift of the spectrum during the extreme overirradiance event, as is observed by its lower average photon energy in comparison to the clear and cloudy sky spectra.
Development and validation of a parameter estimation methodology for two different lithium-ion batteries to optimize their performance and life cycle
Authors: Nakama V , Lafoz M , Nájera J , Sal y Rosas D 
Published: March 2021

Abstract
This paper presents four procedures developed to analyse the dependence of the discharge curve of a lithium battery on discharge current and working temperature. For this work, two models of lithium batteries have been used, whose discharge curves have different shapes. The first one, the shape of the curve describes an almost horizontal line in most of it (type 1), while in the other one, the shape of the curve describes a negative slope in most of it (type 2). Two of the developed procedures are used for discharge curves with different currents but constant temperature and the other two developed procedures are used for discharge curves with different temperatures but constant current. The information for the development of the simulations is obtained from the datasheet of the analysed lithium batteries.
Thermophysical characterization of vernacular wall materials: measurements of specific heat and density
Authors: Molina J O , Ríos H F , Gómez M M , Horn M J 
Published: March 2021

Abstract
This study presents locally implemented measurement arrangements to determine thermophysical properties, such as the density and specific heat of adobe and sillar (ignimbrite), materials widely used in the walls of high Andean houses in Peru. The values of these properties are unknown, but they are crucial as input variables of dynamic simulation programs to determine the thermal behavior and demand of the heating energy of houses. The experimental setup was referenced to international standards, such as ASTM D7263 and ASTM D4611, and used an open-source Arduino microcontroller. The evaluations were also conducted with the transient conditions of heat flow, with adobe and sillar obtaining densities of 1570 ± 62 kg⋅m−3 and 1338 ± 20 kg⋅m−3 and specific heats of 1209 ± 93 J⋅kg−1⋅K−1 and 1800 ± 96 J⋅kg−1⋅K−1, respectively. The results suggest to carry out more measurements, especially of specific heat given the scarce local and international information on vernacular building materials.
Thermal comfort analysis of Ayni house at the “Solar Decathlon Latin America and Caribbean 2015”
Authors: Ocupa D A , Horn M 
Published: March 2021

Abstract
The Ayni house is a prototype designed and constructed by the National University of Engineering of Peru for the “Solar Decathlon Latin America and Caribbean”, realized in 2015 in Cali, Colombia. In this paper the thermal performance of the Ayni house under the parameters of comfort of the competition is presented.
Morpho-physiological features of Stephanotis floribunda inconditions of greenhouse culture
Authors: Turbina I N , Kravchenko I V 
Published: March 2021

Abstract

Stephanotis floribunda Brongn. is an ornamental pot-plant characterized with intensive growth, profuse flowering, stability in interior conditions. We studied the growth and developmental rhythm of the shoot system of the experimental plant, investigated its biomorphological features, compared the physiological and biochemical characteristics of the assimilating organs of Stephanotis floribunda during the growing season in phases: budding, mass flowering, flowering end, single flowering, slow growth and relative peace. The system of stephanotis shoots is characterized by a plagiotropic growth direction and all its vegetative-generative areas are structurally homogeneous. Due to its ability to take root in each node, the structural unit – metamer – retains autonomy and is able to restore the entire individual. The indicators of the pigment composition throughout the whole observation period indicate the stability of the photosynthetic system, and confirm the significant adaptation potential of Stephanotis floribunda in protected ground conditions.
Investigation of the level of endogenous intoxication of the body and the characteristics of the saliva crystallization in poultry with feed toxicosis
Authors: Dolgov E P , Kuzminova E V , Semenenko M P , Osepchuk D V , Vasiliadi O I 
Published: March 2021

Abstract
In the course of the experiments, it was found that during the reproduction of feed toxicosis in broiler chickens caused by prolonged intake of mycotoxins (T-2 toxin and aflatoxin B1), clinical signs of intoxication were recorded starting from the 5th day of the experiment. Disturbances in the biochemical profile of the bird’s blood were manifested by changes in protein metabolism, as well as in indicators reflecting the functioning of the liver and kidneys. The level of endogenous intoxication in the body of broiler chickens with combined mycotoxicosis was significantly higher than the data of intact control at a spectrophotometer wavelength λ = 254 nm - by 14.8%, at λ = 280 nm - by 36.6%, AWM distribution index (DI) 280/254 increased by 18.9%. Comparison of the saliva facies of healthy chickens and chickens with mycotoxicosis revealed differences between the studied crystallograms.
Molecular characteristics isolates of rabies virus isolated from humans in Ukraine
Authors: Polupan I M , Nedosekov V V , Stepanova T V , Rudoi O V , Parshikova A V , Drozdova E I 
Published: March 2021

Abstract
The article presents the results of molecular-genetic investigation of isolates of rabies virus was isolated from 2 rabies patients in Ukraine. It has been confirmed that the virus that caused the deaths belongs to animal lyssavirus, namely the rabies virus. It has been proved that street isolates from humans by their genetic characteristics belong to the first genotype (RABV), the first phylogroup of animal lyssavirus. High homology between experimental samples and materials from cats, dogs, and foxes from the territory of Ukraine and with isolates in fox populations in South-Eastern Europe was revealed. RT-PCR confirmed that 2 people died of rabies, the isolates belong to the first phylogroup of lissavirus, the first genotype (RABV). It is proved that 2 street isolates from people from Ukraine are similar in degree of homology to the isolates isolated in fox populations in South-Eastern Europe.
Pollination and hybridisation systems in genus Ribes L. (Grossulariaceae)
Authors: Yandovka L F , Barabanov I V 
Published: March 2021

Abstract
Currants are important berry crops cultivated for medicinal purposes. Most currant cultivars are crossings between Ribes nigrum varieties, while many Ribes species are not used in hybridisation. Recent research on Ribes crossbreeding is scarce. Hybridisation of wild species can improve reproductive success and the taste of fruit. However, hybrids of certain currant species cannot produce fully formed berries. Crossbreeding of currant species was performed in Northwest Russia (Saint Petersburg Botanical Garden). The research included both introduced (R. sachalinense, R. sanguineum, R. rubrum, R. hispidulum, R. mandschuricum, R. spicatum, R. latifolium) and native species (R. aureum, R. alpinum, R. nigrum). The research aimed to identify the hybridisation system, pollination mechanisms and hybrids with the highest percentage of fruit and seed set. Pollen fertility and viability were assessed. Pollen is fertile (acetocarmin staining: 80.3% for R. alpinum and 96.0% for R. mandschuricum); germination is active on nutrient medium and in situ, especially in R. sachalinense, R. aureum and R. spicatum (54.2–41.8%). Pollen germination can be irregular. Flowers were castrated before pollination. Optimal hybrids with a high percentage of fruit set were identified: R. alpinum x R. mandschuricum, R. alpinum x R. aureum, R. sachalinense x R. sanguineum, R. rubrum, R. hispidulum.
Study of cell culture strains sensitivity to sacbrood virus
Authors: Kalinin A G , Kuleshov K V , Isaev Y G 
Published: March 2021

AbstractA huge number of cell culture strains obtained from one organ (tissue) of an animal, sensitive to causative agents of various infections, are known. However, a comparative characterization of various cell culture strains sensitivity to such bee viruses as SBV has not yet been carried out. This work was carried out in the Department of Cell Biotechnology and the Laboratory of Bee Diseases of the Federal State Budget Scientific Institution “Federal Scientific Centre VIEV”. For the study, we took cell cultures of the African green monkey kidneys (Vero (3 strains) - Vero (strain from the FSC VIEV Collection)), Vero K (strain from the FSC VIEV Collection), Vero (strain from Germany). We have shown that all 3 of the Vero cell cultures above strains are sensitive to SBV, however, the cell model in which the most pronounced signs of CPD were revealed is the Vero K cell line. The first signs of CPD manifestation virus in the culture of Vero K cells were observed at first passage. After 6 days. screeds were found in the monolayer after infection. At the second passage of the virus after 5 days. culturing CPD manifested itself as the appearance of voids, cell strands, and rounding of the cells part.
On the issue of developmental stability of urban tree populations
Authors: Baranov S G , Vinokurov I Y , Zykov I E , Fedorova L V , Biryukova T S 
Published: March 2021

Abstract
Fluctuating asymmetry as an indirect deviation in the difference between the right and the left bilaterally symmetrical traits is considered an indicative symptom of stress. The results of urban populations studying Betula pendula, Tilia cordata, Quercus robur and Acer platanoides using the conventional normalizing method and the geometric morphometrics are presented. The relationship between the environmental stress of an urbanized area and asymmetry values was analyzed. 8-10% of the samples | R – L | / (R + L) had a normal distribution (K-S test, p <0.01). Only one trait in birch and one in linden significantly (p <10−4) reflected the stress effect in a direct relationship. Q. robur and A. platanoides had a wider range of FA, and the traits reflecting stress were different. No correlation was found between the FA and GMFA integral indices. High heterogeneity of values (R – L) was accompanied by the significance of directional asymmetry. The GM method extinguished the kurtosis in trait and showed a significant value of GMFA at kurtosis values γ < 2.5. The high heterogeneity (γ >2.5) accompanied by insignificant fluctuating asymmetry in Procrustes ANOVA. The authors emphasize that the toxic effect of pollutants can be associated with the relief and protection from winds in urbanized areas. The heterogeneity of the genotype of plantings from different nurseries increases the factor diversity, which makes the results of the analysis of developmental stability much more critical.
Nutrigenomics as a tool for optimizing the composition of specialized food products by the efficiency criterion
Authors: Utkina A S , Karagodin V P 
Published: March 2021

Abstract
The article presents the scientific basis for designing the optimal composition of specialized food products applying nutrigenetic and nutrigenomics tools. The effect of functional ingredients on the levels of the target genes expression associated mainly with immunity and adaptation of the organism to physical activity was studied. Caffeine, vitamin D and beta-glucan were applied; their target genes were identified in experiments based on the scientific literature analysis. It was established that the efficiency of vitamin D and caffeine depends on the genotype of the consumer, determined by genetic testing to specify alleles. The individual reaction of consumers to beta-glucan indicates the absence of polymorphism in the target gene. It does not require a personal approach to its consumption. An algorithm of new specialized food products development by including functional ingredients in their composition with the efficiency confirmed by nutrigenomics was developed.
Relationship of barley leaf’s pigment content with development of yield structure elements
Authors: Zaytseva I Yu , Noskova E N , Lisitsyn E M , Schennikova I N 
Published: March 2021

Abstract
Variation in contents of chlorophyll and carotenoid pigments and their correlation links with elements of yield structure of 17 samples of spring barley (Hordeum vulgare L.) was estimated by method of spectrophotometric analysis of acetone extracts from flag and second leaves. The variability of chlorophyll a content was 13.3%, of chlorophyll b – 13.5%, and carotenoids – 14.4% for flag leaf, and respectively 11.6%; 10.8%; 11.2% - for second leaf. Practically there was no variation in distribution of pigments between light-harvesting complexes (LHC) and reaction centers (RC) of photosystems (the average value of a part of chlorophyll in a light-harvesting complex was 81% of its total amount at a variation of 2.8%). The flag leaf pigments’ content was not correlated with final productivity of barley plants (r ≤ 0.27) whereas for a second leaf it is revealed statistically significant (at p ≤ 0.05) interrelation between productivity and content of chlorophyll a (r = 0.64), chlorophyll b (r = 0.58), and carotenoids (r = 0.60). It is disclosed that in the studied set of spring barley samples, one can predict extent of the level of the next parameters: “ear length”, “grain mass per ear” and “1000-grains mass” by the pigments content in a flag leaf; and the parameters “ear length” and productivity – by its content in a second leaf. Among three analyzed photosynthetic pigments, yellow pigments (carotenoids) render the greatest influence on the level of development of separate elements of yield structure. Content of pigments in a second leaf is more suitable index for assessment of potential productivity of spring barley cultivars. Breeding work on increase of carotenoids and/or chlorophyll b contents in a second leaf is perspective.
Bottom sediments of wastewater treatment settling ponds system as a promising complex raw material
Authors: Krasavtseva E A , Svetlov A V , Makarov D V 
Published: March 2021

Abstract
The paper considers the potential possibility of using sediments formed by settled suspended solids from settling ponds of the wastewater treatment system as a complex raw material. Leaching of pre-agglomerated suspensions with 2% sulfuric acid using 30% sulfuric acid as a binder in 90 days made it possible to extract 36% of copper and 55.7% of nickel. The sorption properties of fired granules made with the use of sulfite-alcohol stillage as a binder in relation to copper and nickel ions have been demonstrated. In the first few days of the experiment, it was possible to adsorb 68-73% nickel and 90-92% copper from the model solution supplied to the percolator. The possibility of obtaining a brucite-containing sorbent by hydrochloric acid opening of the initial material of suspensions and its use for purifying model solutions from fluorine ions with an efficiency of up to 62% is shown.
Development of the automated workstation for the operator of the solid municipal wastes landfill
Authors: Sereda T G , Kostarev S N 
Published: March 2021

Abstract
An automated workstation for the operator of the Solid Municipal Waste Landfill was developed on the example of the Solid Municipal Waste landfill in Nytvensky District, Perm Region. The most important task is to automate the accounting of incoming waste for disposal at the Solid Municipal Waste landfill. The system includes wheel-load scales, video locking system, recognition module and database management system. The database management system generates reports of three types: for landfills, for organizations removing waste from residential areas and infrastructure facilities, and for emission flows (leachate and LFG). The developed database management system is intended for accounting of incoming wastes to landfills. In the future, this database will serve as the main one for keeping records of emissions, creating reports on landfill workloads and their filling dynamics. A diagram of states and transitions of operator`s automated workstation was built. The logical equations describing the algorithm of equipment operation are developed. The ladder program for programming the logic controller is based on the international standard IEC 61131-3. A block diagram of an algorithm for solving the task of automated monitoring of processes at the Solid Municipal Waste range, aimed at preventing emissions from getting into the environment, has been proposed. The Omron SYSMAC CP1L controller and NT21 touch screen were chosen as hardware and software. The automated workstation developed for the landfill operator will improve the quality and productivity of the landfill controller and generate reports in both text and graphic form, with their transmission to environmental organizations.
Metals content in deep eutectic solvents-based extracts of Koenigia Weyrichii growing in the Kola Peninsula
Authors: Tsvetov N S , Drogobuzhskaya S V , Korovkina A V 
Published: March 2021

Abstract

Koenigia Weyrichii is a potentially valuable plant for the extraction of flavonoids. Growing in the Polar region, in particular in the Kola Peninsula, this plant accumulates high amounts of flavonoids. However, industrial pollution can lead to an increased content of various metals in plant material and extracts obtained from it. One of the methods of extraction bio-active components from plants is applying deep eutectic solvents. These solvents can extract not only bio-active components but also metals, including toxic ones. In this research extracts of K. Weyrichii were obtained with a mixture of choline chloride + glycerol with the addition of 10 – 50 wt.% of water. 60% v/v ethanol + water mixture was reference extractant. Various metals concentrations in extracts were estimate with mass-spectrometer with inductively coupled plasma. It is obtained that extracts based on deep eutectic solvents are characterized by low concentrations of toxic and rare metals, and relatively high concentrations of macro- and micro-nutrient metals.
Spatial structure of vegetation in Lake Gusinoe (Republic Byriatia)
Authors: Bazarova B B , Kuklin A P 
Published: March 2021

Abstract
Lake Gusinoe is the second largest lake in the Baikal Natural Area (after Lake Baikal). The conducted research presents current water flora and the spatial pattern of vegetation at Lake Gusinoe mainly depend on the littoral structure and anthropogenic stressors. Macrophytes grow up to the depth of 13.0 m on the southwestern shore of the lake. In the other part of the lake, the plants are observed at 8.5-11.0 m. The littoral zone to the depth of 11 m occupies 50.7 per cent of total area of the lake. At present, the reduced areas of Ph. australis are detected with small groups of P. crispus, P. pectinatus on the western shore at the depth of 1.5 m. P. praelongus communities are only found in the littoral opposite the Tobkhor River. Generally, Charophyte and Bryophyta remain predominant in the lake as registered in the previous years. Presumably, the benthic vegetation complex contributes to a stable ecological status. Overgrowing Charophyta and Bryophyta maintain the self-purification of the ecosystem.
Preservation of biodiversity of the genus Hedera L. in the Nikita botanical garden
Authors: Khailenko E V , Plugatar S A , Zykova V K 
Published: March 2021

Abstract
Representatives of the genus Hedera L. have been cultivated in Nikitsky Botanical Gardens over two centuries. The inventory of the collection has revealed thirty five species, cultivars, and forms, including four relics of cultivation – H. helix ‘Angularis’, ‘Rugosa’, ‘Cavendishii’, and H.
hibernica ‘Scutifolia’. The presence of three species, thirty-two cultivars and forms belonging to four species, of which two species and four varieties have reached the generative stage and twenty-six cultivars are at the juvenile stage, has been established. There are seven of nine garden groups in the collection. Within morpho-decorative characteristics, two groups have been identified in terms of the color of the leaf blade: monochromatic green (twenty-four varieties and forms); and variegated (eight varieties and forms, of which there are seven white-spotted and one yellow-spotted varieties). In terms of the color type (multicolored), marginal, median, and suffuse colors have been identified. In terms of the shape of the leaf blades, the following groups have been identified among the species and cultivars: finger-cut, rounded-reniform, ovate-elongated, and irregularly asymmetric. In terms of the growth type at the juvenile stage, most species of the genus proved to be plagiotropic; yet cultivars with orthotropic growth type have also been found (in our collection, this is H. helix ‘Conglomerata’). Promising directions for increasing biodiversity of the genus include their further introduction by way of the generic complex, as well as enlarging the collection, primarily with variegated varieties of different color types and varieties from two garden groups that have not yet been present in the collection.