bluuebunny/crossref_metadata_2025_split huggingface dataset:
Pathologist Concordance for Ovarian Carcinoma Subtype Classification and Identification of Relevant Histologic Features Using Microscope and Whole Slide Imaging
Authors: Gavrielides Marios A. , Ronnett Brigitte M. , Vang Russell , Barak Stephanie , Lee Elsie , Staats Paul N. , Jenson Erik , Skaria Priya , Sheikhzadeh Fahime , Miller Meghan , Hagemann Ian S. , Petrick Nicholas , Seidman Jeffrey D. 
Published: February 2021

Context.—Despite several studies focusing on the validation of whole slide imaging (WSI) across organ systems or subspecialties, the use of WSI for specific primary diagnosis tasks has been underexamined.Objective.—To assess pathologist performance for the histologic subtyping of individual sections of ovarian carcinomas using a light microscope and WSI.Design.—A panel of 3 experienced gynecologic pathologists provided reference subtype diagnoses for 212 histologic sections from 109 ovarian carcinomas based on optical microscopy review. Two additional attending pathologists provided diagnoses and also identified the presence of a set of 8 histologic features important for ovarian tumor subtyping. Two experienced gynecologic pathologists and 2 fellows reviewed the corresponding WSI images for subtype classification and feature identification.Results.—Across pathologists specialized in gynecologic pathology, concordance with the reference diagnosis for the 5 major ovarian carcinoma subtypes was significantly higher for a pathologist reading on a microscope than each of 2 pathologists reading on WSI. Differences were primarily due to more frequent classification of mucinous carcinomas as endometrioid with WSI. Pathologists had generally low agreement in identifying histologic features important to ovarian tumor subtype classification with either an optical microscopy or WSI. This result suggests the need for refined histologic criteria for identifying such features. Interobserver agreement was particularly low for identifying intracytoplasmic mucin with WSI. Inconsistencies in evaluating nuclear atypia and mitoses with WSI were also observed.Conclusions.—Further research is needed to specify the reasons for these diagnostic challenges and to inform users and manufacturers of WSI technology.
Titler, typografi og andre tilpasninger
Authors: Appel Charlotte 
Published: February 2021

Charlotte Appel: Titles, typography and other adjustments. A study of Morten Hallager as a media-savvy publisher of Danish books for children during the last decades of the eighteenth century
This article investigates the involvement of Morten Hallager (1740–1803) in the book business, and how he contributed to shaping a new commodity in Denmark: books for children. Until recently, Hallager has not attracted much scholarly attention due to a traditional focus on authors who made original contributions to Danish literature. However, Hallager’s combined experience as a printer (1771–84), a schoolteacher (from 1785) and an expert in German and French gave him a unique background to act as a transnational agent, introducing European Enlightenment literature for children (by J. H. Campe, C. K. J. Dassel, K. T. Thieme, A. Berquin etc.) to Danish readers.
After an outline of Hallager’s life and career, the article presents a survey of his publications. He was particularly active as an author, translator, compiler and publisher of books for children c.1791–1804 (his last books were published posthumously), and during this period he published 38 individual titles – and 57 editions in all (including 19 second or later editions) – corresponding to c.11 per cent of all Danish titles for a young readership. Four main types of intervention that characterise Hallager’s books for children are analysed. First, he took great care over titles and the contents of title pages. Most of them would include an explicit reference to ‘child’, ‘children’ or ‘youth’, and Hallager would present himself as a schoolteacher and thus an expert in the field. Next, when it came to the physical appearance of the books, Hallager made use of his professional know-how. His initial success, a small reader in sextodecimo from 1791 (reprinted ten times), for example, demonstrated how he made choices concerning format, typeface etc. Third, Hallager made a number of pedagogical adjustments to the translated texts, reflecting his ambition to be as specific and concrete as possible and also to include variety, so that his young readers were never bored. Fourth, the article maps his impressive range of strategies with regard to translating, transforming and ‘localising’ foreign texts, so that they would become more digestible and relevant for a Danish audience.
Finally, the conclusion argues that Hallager’s experience in every role and every position within Robert Darnton’s famous communication circuit (1982) was a key to his success – and may explain his wish to explicate his publishing strategies in great detail. For this reason, a study of Hallager’s publications provides us with new insights not only into his own book business but also into the emerging market for children’s books in general.
Composition and distribution of fish environmental DNA in an Adirondack watershed
Authors: Cornman Robert S. , McKenna, Jr. James E. , Fike Jennifer A. 
Published: February 2021

Background
Environmental DNA (eDNA) surveys are appealing options for monitoring aquatic biodiversity. While factors affecting eDNA persistence, capture and amplification have been heavily studied, watershed-scale surveys of fish communities and our confidence in such need further exploration.

Methods
We characterized fish eDNA compositions using rapid, low-volume filtering with replicate and control samples scaled for a single Illumina MiSeq flow cell, using the mitochondrial 12S ribosomal RNA locus for taxonomic profiling. Our goals were to determine: (1) spatiotemporal variation in eDNA abundance, (2) the filtrate needed to achieve strong sequencing libraries, (3) the taxonomic resolution of 12S ribosomal sequences in the study environment, (4) the portion of the expected fish community detectable by 12S sequencing, (5) biases in species recovery, (6) correlations between eDNA compositions and catch per unit effort (CPUE) and (7) the extent that eDNA profiles reflect major watershed features. Our bioinformatic approach included (1) estimation of sequencing error from unambiguous mappings and simulation of taxonomic assignment error under various mapping criteria; (2) binning of species based on inferred assignment error rather than by taxonomic rank; and (3) visualization of mismatch distributions to facilitate discovery of distinct haplotypes attributed to the same reference. Our approach was implemented within the St. Regis River, NY, USA, which supports tribal and recreational fisheries and has been a target of restoration activities. We used a large record of St. Regis-specific observations to validate our assignments.

Results
We found that 300 mL drawn through 25-mm cellulose nitrate filters yielded greater than 5 ng/µL DNA at most sites in summer, which was an approximate threshold for generating strong sequencing libraries in our hands. Using inferred sequence error rates, we binned 12S references for 110 species on a state checklist into 85 single-species bins and seven multispecies bins. Of 48 bins observed by capture survey in the St. Regis, we detected eDNA consistent with 40, with an additional four detections flagged as potential contaminants. Sixteen unobserved species detected by eDNA ranged from plausible to implausible based on distributional data, whereas six observed species had no 12S reference sequence. Summed log-ratio compositions of eDNA-detected taxa correlated with log(CPUE) (Pearson’s R = 0.655, P < 0.001). Shifts in eDNA composition of several taxa and a genotypic shift in channel catfish (Ictalurus punctatus) coincided with the Hogansburg Dam, NY, USA. In summary, a simple filtering apparatus operated by field crews without prior expertise gave useful summaries of eDNA composition with minimal evidence of field contamination. 12S sequencing achieved useful taxonomic resolution despite the short marker length, and data exploration with standard bioinformatic tools clarified taxonomic uncertainty and sources of error.
Studying the Impact of Total Quality Management on Entrepreneurial Organizations' Performance (Case Study: Insurance Companies in Iran)
Authors: Parsamand Afsaneh 
Published: February 2021

Purpose: Iran's situation in its competitive market and developing economy has encouraged entrepreneurial industrial organizations to improve the quality of their products and services so that they can enter the world's competitive market. To do so, they need to incorporate some effective means, one of which is total quality management. Total quality management is both a culture and a series of strategic principles to ensure consistent progress in entrepreneurial organizations.
Methods: This is a descriptive correlational study. The statistical population is 321 insurance employees in Tehran, Iran; 140 of these employees were randomly selected as a sample group by the use of the Morgan table. The Research tool is the Total Quality Management questionnaire that consists of the Organizational Performance Questionnaire by Kaplan and Norton (2001) and the Total Quality Management questionnaire by Wang et al (2012). Cranach's alpha method was applied to measure reliability, and the results were confirmed. To analyze the research data, inferential statistics were used including Kolmogorov–Smirnov test (K-S) for the normality of the data and the Regression method to test hypotheses. SPSS software was also used to analyze and process the research data.
Conclusion: results indicate that Total Quality Management has a significant influence on organizations' performance including the performance of financial sections, organizations' customers, the internal process, and organizations' learning and progress.
AMMI Biplot Analysis for Genotype X Environment Interaction and Stability for Yield in Hybrid Rice (Oryza sativa L.) Under Different Production Seasons
Authors: Devi K. Rukmini , Venkanna V. , Lingaiah N. , Prasad K. Rajendra , Chandra B. Satish , Hari Y. , Rao P. Jagan Mohan 
Published: February 2021

The aim of the study was to determine the genotype x environment interaction and stability performance of fifteen rice hybrids in three different production seasons during 2016, 2017 at Regional Agricultural Research Station, Warangal, Telangana. Data was subjected to the additive mean effects and multiplicative interaction (AMMI) analysis, results indicated that significant genotype x environmental interaction (GEI) influenced the relative ranking of the hybrids across the seasons. It was evident from AMMI analysis that first two principal components accounted for 94.09%, which is enough to explain the variability among the hybrids. The hybrids, G9 (WGRH 18), G8 (WGRH-17) and G12 (WGRH-22) and G3 (WGRH-10), exhibited high grain yield. The AMMI 2 biplot revealed that the rice genotype, G15 (WGL-14), close to the origin indicated non sensitive nature of this genotype with the seasons and highly stable genotype across the environments with low yield potential when compared to hybrids. Whereas the rice hybrids, G9 (WGRH-18), G8 (WGRH-17), G3 (WGRH-10) and G5 (WGRH-14), were also close proximity to origin and have limited interaction with the seasons. The rice hybrid, G9 (WGRH-18), has high mean yield with stable performance over three environments being the overall best can be considered for the release after through conformation.
Prescribing Practices of Medications in the Outpatient Dermatology Department of a Public Hospital
Authors: Ahmed Nehad J. 
Published: February 2021

Aim: This study aims to describe the prescribing pattern of medications by dermatology outpatient department in a public hospital in Alkharj.
Methodology: This is a retrospective study that included collecting data from outpatient electronic prescriptions in a public hospital in Alkharj. All of the outpatients who received prescriptions written by the dermatology department between 1st of January till 30th of June 2018 were included in the study. The data were collected and analyzed using Microsoft Excel.
Results: A total of 328 patients received outpatient prescriptions written by dermatology department. Most of them were females (62.80%) and aged less than 40 years (67.38%). The most prescribed drug in the present study was Hydrocortisone (14.33%) followed by White Soft Paraffin (8.84), Fusidic acid (8.54%) and Cetirizine (8.23%). Most of the medications were prescribed as ointment (28.66%) followed by creams (28.04%).
Conclusion: Females were found to be more predominant with dermatological diseases when compared to males. The most commonly prescribed drugs were hydrocortisone, white soft paraffin, fusidic acid and cetirizine. It is important to evaluate prescribing pattern of the drugs periodically to improve the quality of prescriptions.
Scopoletin Ameliorates Lipopolysaccharide Induced Neuroinflammation, Oxidative Stress and Cognitive Dysfunction in Mice: A Mechanistic Study
Authors: Gursahani Malvika , Gawali Nitin , Pai Sarayu , Mestry Snehal , Juvekar Archana 
Published: February 2021

Background: Alzheimer’s disease (AD), a progressive neuropsychiatric disorder, is characterized by neuroinflammation, β-amyloid plaques and neurofibrillary tangles. It has been demonstrated that systemic inflammation and neuroinflammation caused by lipopolysaccharide (LPS) injections in rodents leads to elevation in Aβ levels and neuronal cell death, finally resulting in cognitive impairment.
Methods: Animals received the experimental compound – Scopoletin (2.5, 5 or 10 mg/kg, i.p) for 31 days. Initially for 14 days, they received only the experimental drug; thereafter for the next 7 days they received LPS (0.25 mg/kg i.p) along with the experimental molecules.
Treatment with the experimental molecules was continued for the next 10 days and behavioural tests were performed. Animals were sacrificed on day 32 and biochemical tests were performed on brain tissue homogenate.
Results: Scopoletin administration improved memory and cognition as indicated by reduction of escape latency in Morris water maze test and increased number of entries in Y maze test. Malondialdehyde was significantly increased after LPS administration and reduced glutathione, catalyse and superoxide dismutase were significantly reduced in mice brains. Treatment with scopoletin significantly reduced oxidative stress. Inflammatory markers. β-secretase and apoptotic markers were also markedly reduced.
Conclusion: At medium and high dose, Scopoletin showed promise response as a prophylactic entity in the LPS induced neuroinflamation and cognitive dysfunction model in mice.
High Carrying Rate of Extended-Spectrum Beta-Lactamase (ESBL) Producing Enterobacteriaceae by Slaughterhouse Workers in Lomé, Togo in 2019
Authors: Godonou Amivi Mawussi , Gbeasor-Komlanvi Fifonsi Adjidossi , Tsogbale Novissi , Lack Fiali , Dossim Sika , Batawui Komla Batassé , Ameyapoh Yaovi Agbekponou , Ekouevi Koumavi , Dagnra Anoumou Yaotsè , Salou Mounerou 
Published: February 2021

Introduction: Extended-spectrum beta-lactamase-producing Enterobacteriaceae (ESBL) represent a real public health concern because of their spread. The role of agri-food chains in transmitting of digestive ESBL-producing bacterial strains in the community, was demonstrated but little work was done in our settings (Togo, west Africa). The aim of this study was to estimate the rate of digestive carrying ESBL producing enterobacteriacae in slaughterhouse workers in Lomé, Togo.
Material and Methods: This was a cross-sectional study carried out in three slaughterhouses in Lomé. Fresh stools of 60 slaughterhouse workers and socio-demographic data were collected during the period of September to October 2019 after obtaining the consent of each participant.
The bacterial strains of interest were isolated on the selective medium Purple Bromocresol + Ceftazidime at 6µg/l. UriselectÒ and API 20E media were used for identification. Antibiotic susceptibility test was performed in Mueller-Hinton agar plate diffusion method (Kirby Bauer technic) and according to CASFM-EUCAST recommendations.
Results: The digestive carriage rate of ESBL producing enterobacteriacae among professionals of three slaughterhouses of Lomé was 80% (n=48/60). Escherichia coli was the main bacteria 78.2% (n = 43/55) followed by Klebsiella pneumoniae 16.4% (n = 9/55) and Enterobacter cloacae 5.4% (n = 3/55). The antibiotic profile of ESBL producing enterobacteriacae showed resistance to Amoxycillin + Clavulanic Acid (26%), Ticarcillin + Clavulanic Acid (86%), Piperacillin + Tazobactam (14%), Cefoxitin (7%) Ciprofloxacin (63%), Levofloxacin (49%), Nalidixic Acid (42%), Chloramphenicol (33%), Gentamicin (21%), Sulfamethoxazole-Trimetoprim (93%). These bacteria were 100% sensitive to Imipenem, Ertapenem, Amikacin and Fosfomycin.
Conclusion: This study revealed a very high carriage rate of ESBL producing Enterobacteriacae among Slaughterhouse Workers in Lomé. It confirmed the major potential role of the agri-food chains in the spread of ESBL producing bacteria in the Community.
Low‐Overpotential LiF Splitting in Lithiated Fluoride Conversion Cathode Catalyzed by Spinel Oxide
Authors: Wei Kaiyuan , Zhao Yu , Chen Keyi , Sun Ke , Wu Tao , Dong Zhaohui , Cui Yanhua , Zeng Chao , Li Chilin 
Published: February 2021

AbstractConversion chemistry involving LiF splitting is an appealing route toward high capacity cathodes for Li‐ion batteries. In general, cathodes based on LiF splitting need a redox host to provide the driving force for LiF decomposition from the surface. However, most of the systems are still far from practical owing to their limitations, especially the high charge voltage (4.5–5.0 V) needed to fully utilize the capacity and the subsequent low energy efficiency (<80%). The redox host is apparently the key to further reduce the required charge voltage and improve the energy efficiency. Here, a new LiF–NiFe2O4 conversion cathode chemistry is designed and demonstrated with a crystalline spinel oxide redox host. It is demonstrated that the new host effectively improves the surface redox and LiF splitting kinetics, which enable the new cathode to work at much lower charge cutoff voltage (4.2 V) without compromising the delivered capacity and voltage. The LiF–NiFe2O4 composite thin film is able to be discharged with large capacity of 237 mA h g−1 with high energy efficiency (88%), substantial pseudocapacitance contribution (>90%), and satisfactory capacity retention. This result indicates LiF splitting chemistry can become practical by carefully tailoring the redox host chemistry and optimizing the composition.
Nido‐Hydroborate‐Based Electrolytes for All‐Solid‐State Lithium Batteries
Authors: Payandeh SeyedHosein , Rentsch Daniel , Łodziana Zbigniew , Asakura Ryo , Bigler Laurent , Černý Radovan , Battaglia Corsin , Remhof Arndt 
Published: February 2021

AbstractHydroborate‐based solid electrolytes have recently been successfully employed in high voltage, room temperature all‐solid‐state sodium batteries. The transfer to analogous lithium systems has failed up to now due to the lower conductivity of the corresponding lithium compounds and their high cost. Here LiB11H14 nido‐hydroborate as a cost‐effective building block and its high‐purity synthesis is introduced. The crystal structures of anhydrous LiB11H14 as well as of LiB11H14‐based mixed‐anion solid electrolytes are solved and high ionic conductivities of 1.1 × 10−4 S cm−1 for Li2(B11H14)(CB11H12) and 1.1 × 10−3 S cm−1 for Li3(B11H14)(CB9H10)2 are obtained, respectively. LiB11H14 exhibits an oxidative stability limit of 2.6 V versus Li+/Li and the proposed decomposition products are discussed based on density functional theory calculations. Strategies are discussed to improve the stability of these compounds by modifying the chemical structure of the nido‐hydroborate cage. Galvanostatic cycling in symmetric cells with two lithium metal electrodes shows a small overpotential increase from 22.5 to 30 mV after 620 h (up to 0.5 mAh cm−2), demonstrating that the electrolyte is compatible with metallic anodes. Finally, the Li2(B11H14)(CB11H12) electrolyte is employed in a proof‐of‐concept half cell with a TiS2 cathode with a capacity retention of 82% after 150 cycles at C/5.
Surface‐Triggered In Situ Gelation for Tunable Conformal Hydrogel Coating of Therapeutic Cells and Biomedical Devices
Authors: Pham Tung Thanh , Tran Phuong Le , Phung Cao Dai , Nguyen Hanh Thuy , Nguyen Canh Hung , Yong Chul Soon , Kim Jong Oh , Yook Simmyung , Jeong Jee‐Heon 
Published: February 2021

AbstractHydrogel coatings have been proposed as a promising strategy to improve the biocompatibility of therapeutic cells and biomedical devices. However, developed coating methods are only applicable for simple geometries, typical sizes, and limited substrates. In addition, its applications in therapeutic cell encapsulation are hampered by inadequate construction of the hydrogel capsules such as off‐center encapsulation, immense volume, and lack of control over the thickness of capsules. Here, a method called surface‐triggered in situ gelation (STIG) for universal hydrogel coating of multiscale objects ranging from single cells to mini‐organs to biomedical devices with arbitrary shapes and heterogeneous components is reported. By covering cells or devices with calcium carbonate particles, progressive propagation of alginate hydrogel from their surface under the stimulation of GDL is achieved. The thickness of the hydrogel layers can be easily controlled from several micrometers to hundreds of micrometers by adjusting the gelation time and the release rate of calcium ions. Importantly, STIG facilitates accurate, complete, and individual cell encapsulation, which potentially overcomes the pitfalls of conventional strategies. It is further proven that the low‐cost and facile method can potentially lead to advances in different fields by rendering precisely controlled microscale alginate layers on a wide variety of biomedical substrates.
A Polymer Multicellular Nanoengager for Synergistic NIR‐II Photothermal Immunotherapy
Authors: Xu Cheng , Jiang Yuyan , Han Yahong , Pu Kanyi , Zhang Ruiping 
Published: February 2021

AbstractCell‐membrane‐coated nanoparticles (CCNPs) that integrate the biophysiological advantages of cell membranes with the multifunctionalities of synthetic materials hold great promise in cancer immunotherapy. However, strategies have yet to be revealed to further improve their immunotherapeutic efficacy. Herein, a polymer multicellular nanoengager (SPNE) for synergistic second‐near‐infrared‐window (NIR‐II) photothermal immunotherapy is reported. The nanoengager consists of an NIR‐II absorbing polymer as the photothermal core, which is camouflaged with fused membranes derived from immunologically engineered tumor cells and dendritic cells (DCs) as the cancer vaccine shell. In association with the high accumulation in lymph nodes and tumors, the multicellular engagement ability of the SPNE enables effective cross‐interactions among tumor cells, DCs, and T cells, leading to augmented T cell activation relative to bare or tumor‐cell‐coated nanoparticles. Upon deep‐tissue penetrating NIR‐II photoirradiation, SPNE eradicates the tumor and induces immunogenic cell death, further eliciting anti‐tumor T cell immunity. Such a synergistic photothermal immunotherapeutic effect eventually inhibits tumor growth, prevents metastasis and procures immunological memory. Thus, this study presents a general cell‐membrane‐coating approach to develop photo‐immunotherapeutic agents for cancer therapy.