bluuebunny/crossref_metadata_2025_split huggingface dataset:
Circ-FOXM1 Promotes Cell Proliferation, Migration and EMT Process in Osteosarcoma Through FOXM1-Mediated Wnt Pathway Activation
Authors: Zhang Hao , Zhou Qiongqiong 
Published: May 2021

Abstract
Background: As the most common primary bone tumor in adolescents and children, osteosarcoma commonly occurs with high mortality rate and metastasis. Emerging evidence has illustrated that circular RNAs (circRNAs) are important regulatory RNAs that are involved in multiple biological activities of carcinomas. Circ-FOXM1 (hsa_circ_0025033) is a recently found circRNA and promotes the cellular activities of several cancers. However, the function and molecular mechanism of circ-FOXM1 in osteosarcoma have not been interrogated yet. Methods: The qRT-PCR was utilized to test the expression of circ-FOXM1 in osteosarcoma cell lines. Loss-of-function assays including CCK-8, EdU, TUNEL, transwell and western blot assays were conducted to measure cell proliferation, cell migration, EMT process and cell apoptosis. Luciferase reporter assay and RIP assay were utilized to detect the interaction of circ-FOXM1 and RNAs. Results：We discovered the high expression of circ-FOXM1 in osteosarcoma cells. Besides, it was indicated that circ-FOXM1 knockdown inhibited cell proliferation, cell migration and EMT process, as well as induced cell apoptosis of osteosarcoma cells. Furthermore, circ-FOXM1 was discovered to upregulate the expression level of forkhead box M1 (FOXM1) at post-transcriptional level. Moreover, it was proved that circ-FOXM1 sponged miR-320a and miR-320b so as to increase FOXM1 expression. Additionally, circ-FOXM1 could activate Wnt signaling pathway through upregulating FOXM1. In the end, rescue assays certified that FOXM1 overexpression could totally rescue the circ-FOXM1 silence-repressed cellular activities of osteosarcoma cells.Conclusion: Circ-FOXM1 facilitated the progression of osteosarcoma cells via relieving FOXM1 from the inhibition by miR-320a and miR-320b.
Theoretical Investigation of Excited-State Intramolecular Double-Proton Transfer Mechanism of Substituent Modified 1, 3-Bis (2-pyridylimino)-4,7-dihydroxyisoindole in Dichloromethane Solution
Authors: Liu Xiumin , Li Wenzhi , Wang Yuxi , Tao Yaping , Wang Yi , Hou Yingmin 
Published: May 2021

Abstract
Density functional theory (DFT) and time-dependent DFT (TDDFT) methods were used to investigate substituent effects and excited-state intramolecular double-proton transfer in 1, 3-bis (2-pyridylimino)-4, 7-dihydroxyisoindole (BPI-OH) and its derivatives. The results of a systematic study of the substituent effects of electron-withdrawing groups (F, Cl, and Br) on the adjacent sites of the benzene ring were used to regulate the photophysical properties of the molecules and the dynamics of the proton-transfer process. Geometric structure comparisons and infrared spectroscopic analysis confirmed that strengthening of the intramolecular hydrogen bond in the first excited state (S1) facilitated proton transfer. Functional analysis of the reduction density gradient confirmed these conclusions. Double-proton transfer in BPI-OH is considered to occur in two steps, i.e., BPI-OH (N) →BPI-OH (T1) →BPI-OH (T2), in the ground state (S0) and the S1 state. The potential-energy curves for two-step proton transfer were scanned for both the S0 and S1 states to clarify the mechanisms and pathways of proton transfer. The stepwise path in which two protons are consecutively transferred has a low energy barrier and is more rational and favorable. This study shows that the presence or absence of coordinating groups, and the type of coordinating group, affect the hydrogen-bond strength. A coordinating group enhances hydrogen-bond formation, i.e., it promotes excited-state intramolecular proton transfer.
Kinematics of Crustal Deformation along the Central Himalaya
Authors: Sharma Yogendra , Pasari Sumanta , Ching Kuo-En 
Published: May 2021

Abstract
Using an updated set of GPS surface velocities, the present study provides fault
locking behavior and slip rate distribution of the Main Himalayan Thrust (MHT) along
the central Himalaya. The two-dimensional velocity field is inverted through Bayesian
inversion to estimate fault geometry and kinematic parameters of the MHT along the
central Himalaya. The modeling results reveal that: (1) MHT is fully locked in the upper
flat (0-9 km), partially locked along the mid-crustal ramp (15-21 km), and it is creeping
in the deeper flat (> 21 km); (2) there is an insignificant slip rate of MHT
along the locked-to-creeping transition zone, indicating its partially coupled/locked
behavior; (3) along the deeper flat of the MHT, the estimated creeping rate is ~16.3
mm/yr, ~14.7 mm/yr, and ~14.3 mm/yr along western, central, and eastern Nepal,
respectively; and (4) along the MHT on the upper crust, the modeled locking width
turns out to be 97 km, 106 km, and 129 km in the western, central, and eastern Nepal,
respectively. In addition, the posterior probability distribution of the locking width
exhibits a bimodal Gaussian distribution coinciding with the two ramp geometry of the
MHT along the western Nepal. Along the foothills of the Higher Himalaya, the inferred
locking line is also aligned to the estimated maximum shear strain concentration and
observed seismicity along the central Himalaya. With a general agreement to the
previous geodetic results, geological estimates, and background seismicity, our
findings provide a promising avenue of the contemporary crustal deformation along the
Nepal Himalaya. The estimated inversion results in a Bayesian framework exhibit
updated fault kinematics of the MHT and hence provides valuable inputs for seismic
hazard assessment along the central Himalaya.
Detection of Aspergillus Flavus in Wheat Grains Using Anti-Mannoprotein (MP1) and Spore Proteins Polyclonal Antibodies
Authors: Kumari Ranjana , Ghosh Ananta 
Published: May 2021

Abstract
Cell wall mannoprotein (MP1) gene of an aflatoxigenic strain of Aspergillus flavus, isolated from stored wheat grains, was cloned and sequenced. MP1 protein was expressed in E. coli in soluble form and purified. Polyclonal antibodies were raised against recombinant MP1 protein and inactivated spores of this fungus in rabbit, and purified by ammonium sulphate precipitation, Protein A sepharose and antigen affinity chromatography. The minimum concentration of purified mycelial or spore proteins that could be detected by ELISA was determined as 100 ng using 2 µg of these antibodies. The anti-MP1 antibody was found more sensitive than anti-spore protein antibody. Western blot and immunofluorescence analysis showed reactivity of these antibodies to various proteins (30 kDa to 200 kDa) distributed throughout the surface of mycelia and spore of A. flavus. Cross reactivity of these antibodies was detected with fungi belonging to different Aspergillus, Rhizopus and Alternaria species out of fourteen different fungal species tested. In fungal contaminated wheat grains these antibodies could detect presence of as low as 1 µg mycelia or 103 spores per gram of wheat grains using ELISA. The results suggest that the developed antibodies could be successfully applied for the detection of predominant fungal infestation in stored wheat grains.
BRIT1 Inhibits Invasion and Metastasis by Regulating SERPINA5 in Hepatocellular Carcinoma
Authors: Zhao Xuemei , Xin Ming , Fang Jianqiang , Li Ning , Wang Jialing , Zhang Xuelian , Liang Yulong 
Published: May 2021

Abstract
Purpose: BRIT1 (also known as MCPH1) is a DNA repair and tumor suppressor gene, whose alterations might be potentially associated with tumor progression and metastasis in HCC. SERPINA5, also known as Protein C inhibitor, has been reported to be decreased in a variety of human cancers and its decrease is associated with cellular invasion and tumor metastasis. However, little is known about the underlying mechanism of SERPINA5 downregulation and whether BRIT1 deficiency contributed to regulation control of SEPINA5 and invasion and metastasis in HCC.Methods: The expression of BRIT1 and SERPINs in HCC cells or human tumor tissue microarrays were determined by using quantitative real-time PCR, Western blot analysis, or immunohistochemistry. Migratory and invasive capacity of HCC cells were assessed by using in vitro wound-healing and Transwell-based assays or in vivo mouse xenograft tumor model. Dual luciferase reporter assay was performed to measure promoter activity of the SERPINA5 gene. HCC datasets analyzed in this study was performed by using Oncomine and cBioPortal Cancer Genomics with default parameters.Results: In this study we showed that BRIT1 protein was low expressed in HCC cells and tumor tissues. BRIT1 deficiency increased the capacity of migration, invasion and metastasis of HCC in vitro and in vivo; its expression level was positively correlated with that of SERPINA5 in HCC cells and tissue microarray. With the transcription factor E2F1, BRIT1 can upregulated the promoter activity of the SERPINA5 gene in HCC. Conclusion: These findings suggest that BRIT1 deficiency might induce tumor invasion and metastasis of HCC through regulatory control of SERPINA5 with the help of E2F1, and provide convincing evidence that BRIT1/SERPINA5 might be a novel biomarker for HCC metastasis.
Traditional Chinese Medicine Lingguizhugan Decoction Ameliorate HFD-Induced Hepatic-Lipid Deposition in Mice by Inhibiting STING-Mediated Inflammation in Macrophages
Authors: Cao Lin , Xu Erjin , Zheng Rendong , Zhong Rongling , Huang Fei , Ye Juan , Sun Hongping , Fan Yaofu , Xie Shaofeng , Chen Yu , Xu Yijiao , Zhangchen Zhili , Cao Jing , Cao Wen , Liu Chao 
Published: May 2021

Abstract
Background: Stimulator of IFN genes (STING) is highly expressed in the livers of non-alcoholic fatty liver disease(NAFLD) patients and HFD induced NAFLD mice model. The STING signaling induced inflammation has been shown to be a critical role in metabolic disorders, such as NAFLD and Type 2 diabetes. Lingguizhugan decoction (LGZG), a Traditional Chinese herbal decoction, has been applied to treat metabolic disorders for many years. However, whether LGZG can alleviate the progression of NAFLD through inhibiting inflammation is not fully understood. This study was to determine the role of STING-mediated inflammation in the HFD- induced hepatic-lipid deposition treated with LGZG.Methods: The anti-inflammatory and anti- hepatic-lipid deposition effects of LGZG in vivo were detected by H&E staining, immunofluorescence and immunochemistry. Mice bone-marrow-derived macrophages (BMDMs) were co-incubated with palmitic acid-induced lipid deposition HepG2 cell model (BMDM+PA-HepG2) and treated with LGZG.STING-specific agonist or blockers respectively to detect whether the activation of STING-mediated pathway is involved in the anti-hepatocyte lipid deposition effect of LGZG. Mitochondrial DNA was detected by real time PCR. The expression of inflammatory cytokines related to STING-TBK1-NF-κB pathway was detected by western blotting and ELISA.Results: LGZG significantly ameliorated HFD induced hepatic steatosis, alleviated insulin resistance (IR) and reduced the oxidative stress. Furthermore, LGZG reduced hepatic mitochondrial damage and mitochondrial DNA release, which corresponded to reducing the expression of STING as well as the the infiltration of STING-positive Kupffer cells in the liver of HFD fed mice. LGZG directly inhibited the activation of STING-TBK1-NF-κB pathway in BMDM induced by DMXAA, LPS, thereby reducing the release of IFNβ and TNFα. Co-incubation LGZG treated BMDM and PA-stimulated HepG2 significantly reduced PA-induced lipid deposition in HepG2 cells by inhibiting STING- mediated signal pathways. Conclusion: This study demonstrates that LGZG can ameliorate HFD-induced hepatic -lipid deposition through inhibiting STING-TBK1-NF-κB pathway in macrophages, which provides novel insight for elucidating the molecular mechanism of LGZG alleviating HFD induced hepatic steatosis.
Brevilactibacter Soli Sp. Nov., a Novel Member of the Genus Brevilactibacter Isolated from Soil in China
Authors: Li Le Le , Zhao Jiang Yuan , Geng Yu , Zhou Yu Guang , Yuan Hui Ren , Cai Man , Wen Meng Liang , Li Ming Gang , Tang Shu Kun 
Published: May 2021

Abstract
A Gram-stain-positive, cocci-shaped, facultatively anaerobic, non-motile bacterial strain, designated YIM S02567T, was isolated from a forest soil sample collected from Gejiu City, Yunnan Province, south-west PR China. Growth was observed at 10–45°C, at pH 6.0-9.5, in the presence of up to 4.0 % (w/v) NaCl on R2A medium. Through the results of 16S rRNA gene sequence similarity analysis showed that strain YIM S02567T was most closely related to the type strain of Brevilactibacter sinopodophylli (95.38 %) and Propioniciclava tarda (94.67%), phylogenetic analysis based on genome data showed that strain YIM S02567T should be assigned to the genus Brevilactibacter. The cell-wall diamino acid was meso-diaminopimelic acid. The major cellular fatty acids were identified as anteiso-C15:0 and C16:0, and the major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, and two unidentified glycolipids. The predominant menaquinone was MK-9(H4). The genomic DNA G + C content was 71.20 mol%. Based on the polyphasic taxonomic evidence, strain YIM S02567T is assigned to a novel member of the genus Brevilactibacter, for which the name Brevilactibacter soli sp. nov., (type strain YIM S02567T = CCTCC AB 2020128T = CGMCC 1.18504T = KCTC 49478T) is proposed.
High Concordance Between Automatic Identification in Ion S5 and Manual Identification in Miseq During Next-Generation Sequencing for Preimplantation Genetic Testing of Aneuploidy
Authors: Chuang Tzu-Hsuan , Wu Zih-Huei , Kuan Chin-Sheng , Lee Meng-Ju , Hsieh Chia-Lin , Wang Huai-Lin , Lai Hsing-Hua , Chang Yu-Jen , Chen Shee-Uan 
Published: May 2021

Abstract
The Ion S5 (Thermo Fisher Scientific) and Miseq (Illumina) NGS systems are both widely used in the clinical laboratories conducting PGT-A. Each system employs discrepant library preparation steps, sequencing principles, and data processing algorithms. The automatic interpretation via Ion Reporter software (Thermo Fisher Scientific) and the manual interpretation via BlueFuse Multi software (Illumina) for chromosomal copy number variation (CNV) represent very different reporting approaches. Thus, it is intriguing to compare their ability of ploidy detection as PGT-A/NGS system. In the present study, four aneuploid cell lines were individually mixed with a diploid cell line at different aneuploid ratios of 0% (0:5), 10% (1:9), 20% (1:4), 40% (2:3), 50% (3:3), 60% (3:2), 80% (4:1) and 100% (5:0) to assess the sensitivity and specificity for whole chromosomal and segmental aneuploidy detection. The clinical biopsies of 107 blastocysts from 46 IVF/PGT-A cycles recruited between December 2019 and February 2020 were used to calculate the concordance. Initially, the pre-amplified products were divided into two aliquots for different library preparation procedures of each system. Applying with the same calling criteria, automatic identification was achieved through the Ion Reporter, while well-trained technicians manually identified each sample through the BlueFuse Multi. The results displayed that both systems reliably distinguished chromosomal CNV of the mixtures with at least 10% aneuploidy from karyotypically normal samples ([Ion S5] whole-chromosomal duplication: 2.14 vs. 2.05, p-value=0.009, segmental deletion: 1.88 vs. 2.05, p-value=0.003; [Miseq] whole-chromosomal duplication: 2.12 vs. 2.03, p-value=0.047, segmental deletion: 1.82 vs. 2.03, p-value=0.002). The sensitivity and specificity were comparable between the Ion S5 and Miseq ([sensitivity] 93% vs. 90%, p=0.78; [specificity] 100% vs. 100%, p-value=1.0). In the 107 clinical biopsies, three displayed chaotic patterns (2.8%), which could not be interpreted for the ploidy. The ploidy concordance was 99.04% (103/104) per embryo and 99.47% (2265/2277) per chromosome pair. Since their ability of detection were proven to be similar, the automatic identification in Ion S5 system presents comparatively faster and more standardized performance.
The Effect of Several Profenofos® (EC 50%) Insecticides on Cotton Bollworm Helivoverpa Armigera (Hub.) in Golestan Province of Iran.
Authors: Darvish Mojeni Taghi , Golmohammadi Gholamreza 
Published: May 2021

Abstract
Cotton Boll Worm, Helicoverpa armigera an important pest of cotton in Iran in some areas, causing yield, feeding on buds, flowers and bolls. The most important method for pest control is the use of chemical methods. Approximately 30% of the insecticides used worldwide were against H. armigera. In order to compare the effect of commercial formulations of insecticide companies on the control of cotton bollworm, a field experiment was conducted in Hashemabad cotton research station Golestan province on 2019 year. This experiment was used in the form of a randomized complete block design with 10 treatments and 4 replications including 10 formulations of profenofos® from different companies along with the control on the cotton plant as spraying application. Sampling of larvae mortality statistics were determined 3, 7 and 14 days after spraying application and corrected according to Henderson-Tilton formula and analyzed by SAS software. Mean treatments were also compared with Duncan's multiple range tests. According to the results of comparing the average in the Golestan province showed that in 3 days after spraying the profenofos® formulation of Arman Sabz® and Alborz Behsam® companies with 76.7 and 71.5% had the greatest effect. Profenofos formulations of Razi Shimi Khoram® Company and javaneh Rooyesh® with 48.2 and 42% had the least effect. Percentage of effect of Profenofos® poison formulation of other companies in 7 days after spraying the formulation of Sabzavaran® and Bazargan Pars® companies with 83.2 and 81.5% had the highest effect and were in group a. The formulations of Partov Naz® , Arman Sabz® and Glia Shimi® companies with 79, 78.5 and 78% impact were in group b. The formulation of Razi Shimi Khoram® company with 47.5% had the lowest impact and was placed in group c. Therefore, we recommended the formulation of profenofos Sabzavaran® and Bazargan Pars® companies with no significant adverse for controlling of cotton bollworn for the next year.
Simultaneous Removal of Soluble Metal Species and Nitrate from Acidic and Saline Industrial Wastewater in a Pilot Scale Biofilm Reactor
Authors: Mendrinou Panagiota , Hatzikioseyian Artin , Kousi Pavlina , Oustadakis Paschalis , Tsakiridis Petros , Remoundaki Emmanouella 
Published: May 2021

Abstract
Α pilot scale packed-bed biofilm reactor was set up and monitored for the treatment of wastewater originating from the hydrometallurgical recovery of metals from printed circuit boards (PCBs). The wastewater is characterized by: (a) low pH, (b) residual soluble metal species and (c) elevated concentrations of nitrate and chloride originating from the use of nitric and hydrochloric acid as leaching agents. Such wastewater could be treated in a bioreactor capable for the simultaneous removal of metals and nitrates, through complete denitrification, in presence of elevated chloride concentrations. However, the possible inhibitory effects of metals as well as the metals bioprecipitation should be investigated experimentally. Biological denitrification was studied under extreme conditions in the bioreactor inoculated with Halomonas denitrificans: at (a) pH 3-8; (b) metal content (Cu, Ni, Zn and Fe) at 50 mg/L and 100 mg/L, respectively (c) nitrate concentration 750-5,750 mg/L NO3- and (d) chloride concentration 5%-10% as NaCl. According to the results, denitrification proceeds rapidly through the formation of nitrite as intermediate which is sequentially reduced completely to nitrogen. The presence of metals does not affect the denitrification process. Iron, zinc, copper and nickel are sequestered from the wastewater via bioprecipitation. Both goals, namely metals removal and complete reduction of nitrate in presence of elevated concentrations of chloride, were successfully achieved by the treatment scheme. The proposed simple, robust and low-cost biological treatment unit is advantageous compared to the conventional wastewater treatment, based on metal precipitation via chemical neutralization, where the problem of nitrate removal remains unresolved.
Hemicentin-1 is An Essential Extracellular Matrix Component of the Dermal-Epidermal and Myotendinous Junctions
Authors: Welcker Daniela , Stein Cornelia , Feitosa Natalia Martins , Armistead Joy , Zhang Jin-Li , Lütke Steffen , Kleinridders Andre , Brüning Jens C. , Eming Sabine A. , Sengle Gerhard , Niehoff Anja , Bloch Wilhelm , Hammerschmidt Matthias 
Published: May 2021

Abstract
The extracellular matrix (ECM) architecture is composed of supramolecular fibrillar networks that define tissue specific cellular microenvironments. Hemicentins (Hmcn1 and Hmcn2) are ancient and very large members (>600 kDa) of the fibulin family, whose short members are known to guide proper morphology and functional behavior of specialized cell types predominantly in elastic tissues. However, the tissue distribution and function of Hemicentins within the cellular microenvironment of connective tissues has remained largely unknown. Performing in situ hybridization and immunofluorescence analyses, we found that Hmcn1 and Hmcn2 show a complementary distribution throughout different tissues and developmental stages. In postnatal dermal-epidermal junctions (DEJ) and myotendinous junctions (MTJ), Hmcn1 is primarily produced by mesenchymal cells (fibroblasts, tenocytes), Hmcn2 by cells of epithelial origin (keratinocytes, myocytes). Hmcn1-/-mice are viable and show no overt phenotypes in tissue tensile strength and locomotion tests. However, transmission electron microscopy revealed ultrastructural basement membrane (BM) alterations at the DEJ and MTJ of Hmcn1-/-mice, pointing to a thus far unknown role of Hmcn1 for BM and connective tissue boundary integrity.
Immunogenicity and safety of an inactivated virus vaccine against SARS-CoV-2 in patients with autoimmune rheumatic diseases
Authors: Bonfa Eloisa , Medeiros-Ribeiro Ana , Aikawa Nadia , Saad Carla , Yuki Emily , Pedrosa Tatiana , Fusco Solange , Rojo Priscila , Pereira Rosa , Shinjo Samuel , Andrade Danieli , Sampaio-Barros Percival , Ribeiro Carolina , Deveza Giordano , Martins Victor , Silva Clovis , Lopes Marta , Duarte Alberto , Antonangelo Leila , Sabino Ester , Kallas Esper , Pasoto Sandra Sandra G. 
Published: May 2021

Abstract
CoronaVac(SARS-CoV-2 inactivated vaccine) has been largely used as the main immunogen for COVID-19 in several countries. However, its immunogenicity in immunocompromised individuals has not been established. This was a prospective controlled study of 910 adult ARD patients and 182 age- and sex-matched control group(CG) who received two doses of CoronaVac in a 28-days interrval. Anti-SARS-Cov-2 IgG and neutralizing antibodies were assessed at each vaccine shot and 6 weeks after the 2nd dose. Vaccine adverse events(AE) were similar in both groups. We observed significant lower anti-SARS-Cov-2 IgG seroconversion(70.4% vs. 95.5%,p < 0.001) and titers[12.1(95%CI 11.0-13.2) vs. 29.7(95%CI 26.3–33.5),p < 0.001], frequency of neutralizing antibodies(56.3% vs. 79.3%),p < 0.001) and median (interquartile range) neutralization activity [58.7(43.1–77.2) vs. 64.5(48.4–81.4),p = 0.013] in ARD patients compared to CG. A significant decline in the number of COVID-19 cases (p < 0.0001) were observed 10 days after the second dose, with a predominant P1 variant. Safety analysis revealed no moderate/severe AEs. In conclusion, CoronaVac has an excellent safety profile and reasonable rates of quantitative serology(70.4%)/neutralization(56.3%) in ARD patients. The impact of this reduced immunogenicity in vaccine effectiveness warrants further evaluation.