bluuebunny/crossref_metadata_2025_split huggingface dataset:
Clinical, Histopathologic, and Cytologic Diagnosis of Mucosal Leishmaniasis and Literature Review
Authors: Daneshbod Yahya , Oryan Ahmad , Davarmanesh Mehdi , Shirian Sadegh , Negahban Shahrzad , Aledavood Azita , Davarpanah Mohammad Ali , Soleimanpoor Hossein , Daneshbod Khosrow 
Published: February 2021

Abstract
Context.—Mucosal leishmaniasis (ML) is a rare disease in the world, even in endemic areas such as Iran. Clinical, histologic, or cytologic assessment may help in the diagnosis of ML.
Objective.—To describe clinical, histologic, and cytologic findings in ML.
Design.—Review of our files showed 11 patients diagnosed with ML, of whom 7 patients had oral lesions, 1 of whom was a known patient with oral leishmaniasis with recurrence of oral lesions; 2 had laryngeal lesions; and 3 had nasal lesions. One case of laryngeal leishmaniasis was a recurrence of prior oral lesions. Cytologic smears were prepared by scraping the lesions with a scalpel or cytobrush. Histology on the biopsies was done for 7 patients. In 2 patients with nasal lesions, exfoliative cytology was made by washing the nasal cavity. Smears were both air dried and fixed in alcohol and stained.
Results.—Cytologic findings showed free Leishman-Donovan bodies, intrahistiocytic Leishman-Donovan bodies, atypical organisms, granuloma, acute and chronic inflammatory cells, histiocytes, multinucleated giant cells, mast cells, binucleated histiocytes (Reed-Sternberg–like cells), and plasma cells. In 6 of the patients, biopsy was inconclusive and in subsequent cytology the organism was detected. In 3 cases, findings from clinical and cytologic examinations were suggestive for leishmaniasis; however, with response to treatment, the diagnosis was confirmed. In 5 patients a malignant tumor was suspected because of clinical or histologic findings, but cytology helped to diagnose leishmaniasis.
Conclusions.—Clinically or histologically, ML can be mistaken for benign and malignant lesions. Scraping or exfoliative cytology is an easy, reliable, and cost-effective method for diagnosing ML. Thus, clinical, histologic, and cytologic features together may help in ML diagnosis.
Nephrogenic Adenoma: Report of a Case and Review of Morphologic Mimics
Authors: Kunju Lakshmi P. 
Published: February 2021

Abstract
Nephrogenic adenoma, also referred to as nephrogenic metaplasia, is an uncommon benign lesion of the urothelial tract, characterized by a circumscribed proliferation of tubules, cysts, and papillae lined by cells with low cuboidal to columnar epithelium. The diagnostic features that are useful in the recognition of this benign entity are the characteristic mixture of various architectural patterns, associated stromal edema and inflammation, hyaline sheath around tubules, eosinophilic colloidlike secretion within tubules, and lack of mitotic activity. Nephrogenic adenoma can be a significant diagnostic pitfall as certain histologic features, such as the presence of enlarged nuclei with prominent nucleoli, degenerative nuclear atypia, tiny tubules with blue mucin simulating signet ring cells, and focal invasion into superficial muscle, when taken out of context, can mimic malignancy. Herein, I report a case of nephrogenic adenoma with some worrisome histologic features and review the diagnostic criteria as well as pertinent morphologic malignant mimics of nephrogenic adenoma.
Controversies in the Diagnosis of Barrett Esophagus and Barrett-Related Dysplasia: One Pathologist's Perspective
Authors: Goldblum John R. 
Published: February 2021

Abstract
Context.—Pathologists frequently assess esophageal biopsy specimens to “rule out Barrett esophagus,” as well as to assess for the presence or absence of dysplasia.
Objective.—To review some of the recent controversies in the diagnosis of Barrett esophagus and Barrett-related dysplasia.
Data Sources.—Sources were the author's experience and review of the English literature from 1978 to 2009.
Conclusions.—Although goblet cells are required by the American College of Gastroenterology to confirm a diagnosis of Barrett esophagus, this definition might expand to include columnar-lined esophagus without goblet cells. The recognition of dysplasia in Barrett esophagus remains a difficult task for the surgical pathologist, with difficulties in distinguishing reactive epithelium from dysplasia, low-grade dysplasia from high-grade dysplasia, and even high-grade dysplasia from intramucosal adenocarcinoma.
Multiparameter Flow Cytometry in the Diagnosis and Management of Acute Leukemia
Authors: Peters John M. , Ansari M. Qasim 
Published: February 2021

Abstract
Context—Timely and accurate diagnosis of hematologic malignancies is crucial to appropriate clinical management. Acute leukemias are a diverse group of malignancies with a range of clinical presentations, prognoses, and preferred treatment protocols. Historical classification systems relied predominantly on morphologic and cytochemical features, but currently, immunophenotypic, cytogenetic, and molecular data are incorporated to define clinically relevant diagnostic categories. Multiparameter flow cytometry provides rapid and detailed determination of antigen expression profiles in acute leukemias which, in conjunction with morphologic assessment, often suggests a definitive diagnosis or a narrow differential. Many recurrent molecular or cytogenetic aberrations are associated with distinct immunophenotypic features, and therefore flow cytometry is an important tool to direct further testing. In addition, detection of specific antigens may have prognostic or therapeutic implications even within a single acute leukemia subtype. After initial diagnosis, a leukemia's immunophenotypic fingerprint provides a useful reference to monitor response to therapy, minimal residual disease, and recurrence.
Objective—To provide an overview of the application of flow cytometric immunophenotyping to the diagnosis and management of acute leukemias, including salient features of those entities described in the 2008 World Health Organization classification.
Data Sources—Published articles pertaining to flow cytometry, acute leukemia classification, and experiences of a reference flow cytometry laboratory.
Conclusion—Immunophenotypic evaluation is essential to accurate diagnosis and classification of acute leukemia. Multiparameter flow cytometry provides a rapid and effective means to collect this information, as well as providing prognostic information and a modality for minimal residual disease evaluation.
Estrogen Receptor β
Authors: Younes Mamoun , Honma Naoko 
Published: February 2021

Abstract
Context—A new class of estrogen receptors was discovered in 1996 and named estrogen receptor β (ER-B); the traditional estrogen receptor, which until a little more than 10 years ago was thought of as the only estrogen receptor in existence, is now called estrogen receptor α. Estrogen receptor β has at least 5 isoforms, which may have different functions and have different tissue distribution. The significance of ER-B expression in tumors was first demonstrated in breast cancer, with several studies demonstrating that women with ER-B–positive breast cancers treated with adjuvant tamoxifen have better survival, independent of estrogen receptor α expression. Pathologists need to be more aware of this increasingly important protein, as it will soon find its way into routine clinical practice.
Objective—To provide pathologists with a concise review of ER-B, with special emphasis on current and potential clinical relevance.
Data Sources—A search of the English literature in PubMed (National Library of Medicine, Bethesda, Maryland) for articles with titles including “estrogen receptor beta,” with emphasis on “immunohistochemistry.” Abstracts were reviewed, and selected articles were used as the basis for writing this review, mostly based on their relevance to pathology.
Conclusions—Estrogen receptor β and its isoforms have wider tissue distribution, including the gastrointestinal tract, lung, and brain, than the traditional estrogen receptor, now called estrogen receptor α. Estrogen receptor β expression in breast cancer is associated with favorable outcome in women treated with adjuvant tamoxifen, even in tumors negative for estrogen receptor α. The clinical significance of ER-B expression in tumors other than breast is currently under investigation.
HER2: Biology, Detection, and Clinical Implications
Authors: Gutierrez Carolina , Schiff Rachel 
Published: February 2021

Abstract
Context—HER2 is a membrane tyrosine kinase and oncogene that is overexpressed and gene amplified in about 20% of breast cancers. When activated it provides the cell with potent proliferative and antiapoptosis signals and it is the major driver of tumor development and progression for this subset of breast cancer. When shown to be overexpressed or amplified by appropriate methods, HER2 is a valuable treatment target.
Objectives—To review the basic biology of the HER2 signaling network, to discuss various approved methods for its detection in clinical specimens, and to describe the impressive results of therapies targeting HER2.
Data Sources—Selected literature searchable on PubMed as well as older studies revealed by the literature review were reviewed.
Conclusion—HER2 is an important member of a complex signaling network and when gene amplified, it results in an aggressive subtype of breast cancer. Patients with tumors found to overexpress HER2 protein or to be amplified for the gene are candidates for therapy that significantly reduces mortality.
Update on Precursor and Early Lesions of Hepatocellular Carcinomas
Authors: Park Young Nyun 
Published: February 2021

Abstract
Context.—There is increasing evidence to support a multistep model of the process of human hepatocarcinogenesis. Precursor lesions are characterized by the appearance of dysplastic lesions in the form of microscopic dysplastic foci and macroscopic dysplastic nodules. There are 2 types of small hepatocellular carcinoma (HCC) (≤2 cm in diameter): (1) early HCC with an indistinct margin and (2) progressed HCC with a distinct margin. Pathologic diagnostic criteria for early HCC have recently been set up based on a consensus between Eastern and Western pathologists.
Objective.—To review the nomenclature, pathology, and biomarkers of precursor and early lesions of HCC.
Data Sources.—Literature review and illustrations from case materials were used.
Conclusions.—Dysplastic foci are composed of large and small cell changes. Small cell change is considered to be a more advanced precursor lesion than large cell change, and large cell change is a rather heterogeneous lesion that may represent both reactive change and true dysplasia. Dysplastic nodules can be categorized as low or high grade according to the degree of atypia. High-grade dysplastic nodules have been reported to show molecular changes similar to HCC and have a high risk of malignant transformation. Early HCC, which may correspond to microinvasive carcinomas of other organs, is a well-differentiated HCC, and differential diagnosis between early HCC and high-grade dysplastic nodule is difficult. Identification of stromal invasion and application of a panel of markers (glypican-3, heat shock protein 70, and glutamine synthetase) is helpful for diagnosis of early HCC. Detection of precursor lesions of HCC is important in recognizing patients with higher risk of developing HCC, and diagnosis of early HCC can improve patient survival by allowing for early and adequate treatment.
Molecular Diagnostics in Urologic Malignancies: A Work in Progress
Authors: Netto George J. 
Published: February 2021

AbstractContext.—Molecular diagnostic applications are now an integral part of the management algorithms of several solid tumors, such as breast, colon, and lung. In stark contrast, the current clinical management of urologic malignancies is lagging behind. Clinically robust molecular tests that can identify patients who are more likely to respond to a given targeted agent or even those in need of a more aggressive treatment based on well-validated molecular prognosticators are still lacking. Several promising biomarkers for detection, prognosis, and targeted therapeutics are being evaluated.Objective.—To discuss candidate biomarkers that may soon make the transition to clinical assay for patients in urologic oncology.Data Sources.—Selected original articles published in the PubMed service of the US National Library of Medicine.Conclusions.—Recent understanding of the complex molecular alterations involved in the development and progression of urologic malignancies is yielding novel diagnostic and prognostic molecular tools and opening the doors for experimental targeted therapies in these prevalent, frequently lethal solid tumors.
“A random system”
Authors: Lønning Moa Nyamwathi , Laupstad Inga , Bringedal Houge Anette , Aasnes Ann Evy 
Published: February 2021

Introduction: This article addresses the provision of rehabilitation services for torture victims with a refugee background in Norway. It engages the topic from the outset of relevant rehabilitation rights and duties, presenting the organisation of rehabilitation services within the Norwegian health care system, and exploring the challenges and opportunities professionals see and experience as they seek to provide adequate treatment and rehabilitation for torture victims.
Methods and material: The article is based on qualitative interviews with 46 experts and practitioners that contribute to or otherwise focus on treatment and rehabilitation for torture victims in Norway, conducted between March and August 2019. Findings and discussion: Rehabilitation services for torture victims in Norway are fragmented, and the resulting practice is highly person dependent. Public services are characterised by insufficient knowledge about torture injuries and rehabilitation and a generalised lack of familiarity with international protocols for identification and documentation of torture. Moreover, the quality of rehabilitation services suffers from a lack of coordination and inclusion of actors that can contribute to comprehensive rehabilitation processes.
Conclusion: Individuals with a refugee background are far from guaranteed adequate rehabilitation for torture-related injuries in Norway. Rehabilitation services suffer from the absence of a systematic approach to identification and documentation, and an unclear division of responsibilities. Three recommendations are proposed in order to ensure minimum standards in rehabilitation services for this group: 1) developing and implementing a national plan of action on torture rehabilitation; 2) knowledge and capacity-building within relevant educational programmes, the national health services and other relevant public sector services; and 3) strengthening and institutionalising interdisciplinary communities of practice with specialised expertise on the topic at all relevant levels.
Effect of incubation temperature and substrate moisture on embryonic development, hatchling phenotypes and post-hatching growth in the Reeves’ Turtle, Mauremys reevesii
Authors: Wei Yufeng , Gao Yangchun , Cao Dainan , Ge Yan , Shi Haitao , Gong Shiping 
Published: February 2021

Background
Reeves’ Turtles (Mauremys reevesii) are economically important in aquaculture in China. Understanding the effects of incubation temperature and substrate moisture on embryos and hatchlings is of great significance for improving the artificial culture of M. reevesii. However, available studies have not yet determined the thermal and hydric optima for M. reevesii eggs, and the potential interaction between the two factors.

Methods
In this study, eggs of M. reevesii were incubated at five temperature levels (23, 26, 29, 32 and 35 °C, fluctuation range ± 0.5 °C). In each temperature level, there were three substrate moisture levels (1:0.5, 1:0.9 and 1:1.2, weight ratio of vermiculite to water). Thus, a total of 15 combinations of temperature and moisture were used to examine the effects of incubation temperature and substrate moisture on incubation duration, hatching success, hatchling phenotypes, post-hatching growth and hatchling survival.

Results
Substrate moisture did not significantly affect most development parameters (except incubation duration and carapace width of hatchlings). Eggs incubated at low moisture level (1:0.5) had a longer incubation duration and produced hatchlings with smaller carapace widths than those incubated at medium (1:0.9) or high (1:1.2) moisture levels. Incubation temperature had a significant effect on incubation duration, hatching success, hatchling phenotypes and hatchling survival. Incubation duration decreased as incubation temperature increased. Eggs incubated at 23, 26 and 29 °C showed higher hatching success than those incubated at 32 and 35 °C. Hatchlings incubated at 32 °C were smaller in body size and mass than those incubated at 23, 26 and 29 °C. At 12 months of age, incubation temperature had no long-lasting effect on body mass, but hatchlings incubated at 23 and 35 °C had lower survival rates than those incubated at 26, 29 and 32 °C. For the development of embryos and hatchlings, the interaction between incubation temperature and substrate moisture was not significant.

Conclusions
Our results indicate that incubation temperature has a significant influence on the development of embryos and hatchlings of M. reevesii, while substrate moisture only significantly affects the incubation duration and carapace width of hatchlings. The combination of an incubation temperature of 29 ± 0.5 °C and a substrate moisture level of 1:1.2 represented optimal incubation conditions in this experiment. Such incubation conditions are helpful in obtaining higher hatching success, shorter incubation duration and higher survival rates for this aquaculture species.
Prognostic implications of cell division cycle protein 45 expression in hepatocellular carcinoma
Authors: Yang Chen , Xie shufang , Wu Yi , Ru Guoqing , He Xianglei , Pan Hong-yin , Wang Shibing , Tong Xiangmin 
Published: February 2021

Background
The overall prognosis of hepatocellular carcinoma (HCC) is poor and novel prognostic biomarkers might better monitor the progression of HCC. Cell division cycle protein 45 (CDC45) plays a key role in DNA replication and considered to be involved in tumorigenesis. This study investigated CDC45 expression in tumour tissues and defined its prognostic value in HCC patients.

Methods
We used immunohistochemistry (IHC) staining to examine the expression of CDC45 in tumour tissue specimens and compare them with adjacent normal tissue specimens using a constructed tissue microarray (TMA) and analyzed how clinical features are related to HCC prognosis. Functional enrichment analyses were used to describe significantly involved hallmark pathways of differentially expressed genes (DEGs, which were screened out according to the high or low expression of CDC45 in tumour tissues).

Results
Our findings showed that the proteome expression of CDC45 was evidently downregulated in HCC tissues compared with matched normal tissues (P < 0.0001). Although we did not find any differences in terms of vascular invasion, metastasis, lymphatic infiltration, or Edmondson grade between patients with high and low CDC45 expression, low CDC45 expression was significantly correlated with microvascular invasion (P = 0.046). Multivariate analysis indicated that CDC45 expression (P = 0.035) was an independent prognostic factor for the overall survival (OS) rate of HCC patients. Patients with CDC45 expression was positively correlated with OS rates among HCC patients (P < 0.05). Functional annotations indicated that CDC45 is involved in the most significant pathways, including the cell cycle, DNA replication, chemical carcinogenesis and drug metabolism–cytochrome P450 pathways.

Discussion
Our findings showed that low proteomic level of CDC45 was associated with a poor prognosis in HCC patients, indicating that CDC45 might be a novel prognostic marker.
Nerica Rice Technology Acquisition through Community Agriculture Development Project in Semi Arid Lands (CADSAL) of Kerio Valley, Kenya
Authors: Lagat Rael J. , Bunyatta David , Rop Nicholas 
Published: February 2021

The aim of Community Agriculture Development Project in Semi-Arid Lands (CADSAL) in Kerio valley was to increase agricultural production in the project area and it used Community Participatory Extension (CPE) approach. A survey methodology was utilized in the study with target population of 480 small-scale farmers who interacted with the project in Kerio valley locations. The objective of the study was to determine and compare the level of performance between the CADSAL participants and non-CADSAL participants in knowledge acquired in NERICA technologies in Kerio Valley of Elgeyo Marakwet County, Kenya. A sample of 160 comprising 80 CADSAL-Participants and 80 Non-CADSAL participants was chosen using simple random sampling. A structured questionnaire was administered through personal interviews to the respondents at their homes. Descriptive statistics was used to compute percentages, means and standard deviations. Inferential statistics was used which included multiple linear regression and t-test to test the hypothesis at α = 0.05. Data was analyzed using Statistical Package for SocialSciences (SPSS) Version 24.0. The result indicated positive acquisition of NERICA technologies by the community using community participatory extension approach. The t-test p-value of less than 0.05 was achieved upon analysis of data which led to rejection of all the null hypothesis. This means further that community extension approach was key in enhancing the level of acquisition of technology by the farmers and hence extension agents need to utilize participatory approach. The study outcome therefore encourages Community participation in the projects meant to increase knowledge of food production in order to increase food production, accessibility and income both in productivity and diversity of production hence sustainability.
The Effects of Social Media on Pharmacy Students’ Academic Performances
Authors: Ahmed Nehad J. , Alrawili Abdulrahman S. , Alkhawaja Faisal Z. 
Published: February 2021

Background: The use of social media by students helps to have access to basic information as quick as possible but numerous universities and schools around the world restrict the access to social networks within its buildings to help students to concentrate on their studies.
Aim: The aim of this article is to assess the impact of social media on academic performance of pharmacy students.
Methodology: The present study includes gathering data from students through a survey research design to determine the effect of social media usage on their academic performance. The survey was adapted from a previous study and after that it is converted to online Google Form.
Results: Most of the participants in the present study said that their academic achievement is excellent (41.67%) or very good (41.67%). The majority of the students agree that the use of social media is useful in educational institutions, social media can be used to arrange group discussions with the experts and that it can be used to fix an appointment with other subject experts. The majority of the students also agree that they can receive announcements from lecturers and faculty using social media.
Conclusion: The majority of the students feel that social media affect their academic performance positively and about half of them feel that social media affect their performance negatively. It is important to increase their awareness about the importance of regulating social media usage.