bluuebunny/crossref_metadata_2025_split huggingface dataset:
Dlk1-Dio3 cluster miRNAs regulate mitochondrial functions in Duchenne muscular dystrophy
Authors: Vu Hong Ai , Bourg Nathalie , Sanatine Peggy , Poupiot Jerome , Charton Karine , Gicquel Evelyne , Massourides Emmanuelle , Spinazzi Marco , Richard Isabelle , Israeli David 
Published: October 2021

AbstractBackgroundDuchenne Muscular Dystrophy (DMD) is a severe muscle disease caused by impaired expression of dystrophin. While mitochondrial dysfunction is thought to play an important role in DMD, the mechanism of this dysfunction remains to be clarified. We recently identified in DMD and in other muscular dystrophies the upregulation of a large number of the Dlk1-Dio3 clustered miRNAs (DD-miRNAs), in both the muscle and the serum. The objective of the present study was to define the biological functions of DD-miRNAs in skeletal muscle, particularly in the context of muscular dystrophy.MethodsDD-miRNAs expression pattern was characterized in vitro and in vivo, in normal and dystrophic situations. Epigenomic characterization was performed, to elucidate the molecular control of DD-miRNAs dysregulation. The biological effect of muscle DD-miRNAs dysregulation was investigated by an in vivo simultaneous overexpression of 14 DD-miRNAs in the wild-type muscle, together with CRISPR-Cas9-based knockdown of the entire DD-miRNA cluster in an iPS-derived myotubes. Omics data and bioinformatics tools were used for the prediction of DD-miRNAs biological functions, and functional characterization of mitochondrial pathways was performed.ResultsWe found that DD-miRNAs dysregulation is not specific to DMD since observed in mouse models for other muscular dystrophies. We showed that DD-miRNAs expression in mdx, is reduced in satellite cells, but highly upregulated in regenerating myofibers, suggesting a myofibers origin of DD6miRNA upregulation in muscular dystrophy in both muscles and serum. We demonstrated that upregulation of DD-miRNAs in the dystrophic muscle is controlled epigenetically by DNA and histone methylation (p<0.0001 and p=0.001, respectively) at the Intergenic Differentially Methylated Region (IG-DMR) of Dlk1-Dio3 locus. Transcriptomic analysis revealed a substantial overlap between the dystrophic muscle of the mdx mouse and the normal muscle that overexpressed 14 DD-miRNAs. Bioinformatics analysis predicted that DD-miRNAs could regulate mitochondrial functions. The ectopic overexpression of 14 DD-miRNAs, in the healthy muscle, resulted in a drastic downregulation of mitochondrial oxidative phosphorylation (OxPhos) (NES=-2.8, p=8.7E-17), similarly to the level in dystrophic muscles of mdx mice and DMD patients (NES=-2.88, p=7.7E-28). Knocking down the entire DD-miRNA cluster in iPS-derived myotubes resulted in increased mitochondrial OxPhos expression and activities.ConclusionsThe present study provides evidence for the modulation of mitochondrial activity in the dystrophic muscle by the upregulated DD-miRNAs and supports an updated model for mitochondrial dysfunction in DMD. The regulation of mitochondrial OxPhos by DD-miRNAs may have a broader impact beyond DMD in physiological and pathological situations of muscle adaptation and regeneration.
CPExtract, a Software for the Automated Tracer-Based Pathway Specific Screening of Secondary Metabolites in LC-HRMS Data
Authors: Seidl Bernhard , Schuhmacher Rainer , Bueschl Christoph 
Published: October 2021

ABSTRACTThe use of stable isotopically labeled tracers is a long-proven way of specifically detecting and tracking derived metabolites through a metabolic network of interest. While recently developed stable isotope assisted methods and associated, supporting data analysis tools have greatly improved untargeted metabolomics approaches, no software tool is currently available that allows to automatically search LC-HRMS chromatograms for completely free user-definable isotopolog patterns expected for the metabolism of labeled tracer substances.Here we present Custom Pattern Extract (CPExtract), a versatile software tool that allows for the first time the high-through-put search for user-defined isotopolog patterns in LC-HRMS data. The patterns can be specified via a set of rules including the presence or absence of certain isotopologs, their relative intensity ratios as well as chromatographic co-elution. Each isotopolog pattern satisfying the respective rules is verified on a MS-scan level and also in the chromatographic domain. The CPExtract algorithm allows the use of both labeled tracer compounds in non-labeled biological samples as well as a reversed tracer approach, employing non-labeled tracer compounds along with globally labeled biological samples.In a proof of concept study we searched for metabolites specifically arising from the malonate pathway of the filamentous fungi Fusarium graminearum and Trichoderma reesei. 1,2,3-13C3-malonic acid diethyl ester and native malonic acid monomethyl ester were used as tracers. We were able to reliably detect expected fatty acids and known polyketides. In addition, up to 189 and 270 further, unknown metabolites presumably including novel polyketides were detected in the F. graminearum and T. reesei culture samples respectively, all of which exhibited the user-predicted isotopolog patterns originating from the malonate tracer incorporation.The software can be used for every conceivable tracer approach. Furthermore, the rule sets can be easily adapted or extended if necessary. CPExtract is available free of charge for non-commercial use at https://metabolomics-ifa.boku.ac.at/CPExtract.
Soil viral communities are structured by pH at local and global scales
Authors: Lee Sungeun , Sorensen Jackson W. , Walker Robin L. , Emerson Joanne B. , Nicol Graeme W. , Hazard Christina 
Published: October 2021

AbstractViruses shape microbial community structures, impacting metabolic pathways and influencing biogeochemical cycles. Despite their importance, the influence of biotic and abiotic factors on viral community structures across environmental gradients in soil is relatively unknown compared to their prokaryotic hosts. While soil pH strongly influences microbial community structure, it is unclear whether there is a similar influence on soil virus communities. In this study, prokaryotic and viral communities were characterized in soils sampled from the extremes of a long-term pH-manipulated soil gradient (pH 4.5 and 7.5), and viral populations were compared to those in a variety of soil ecosystems ranging in pH (4.0 – 7.5). Prokaryotic and viral community structure were significantly influenced by soil pH at the local scale. Of 1,910 viral operational taxonomic units (vOTUs), 99% were restricted to pH 4.5 or 7.5 soil only. These were compared in gene sharing networks of populations from six other European and North American soil systems. A selection of viral clusters from acidic and neutral pH soils were more associated with those from the local gradient pH 4.5 or 7.5 soils, respectively. Results indicate that as with prokaryotes, soil pH is a factor structuring viral communities at the local and global scale.
Motivations, self-congruity and restaurant innovativeness as antecedents of a creative-food tourism experience: the moderating effect of first-time and repeat tourists
Authors: Gómez-Rico Mar , Molina-Collado Arturo , Santos-Vijande María Leticia , Bilgihan Anil 
Published: October 2021

PurposeThis study aims to analyze the drivers of a creative food tourism experience (CFTE) and its effect on the tourists' perceived authenticity and satisfaction. Specifically, this study captures the importance to explain a CFTE of two sets of stimuli: internal stimuli, including push motivations for food travel (i.e. emotional, cultural and social) and the tourist self-congruity (i.e. actual and ideal) with the chosen food establishment; and external stimuli, referred to the pull motivation exerted by restaurant innovativeness.Design/methodology/approachData are obtained through a questionnaire completed by 407 food tourists who have traveled to visit a creative restaurant. The research model is tested using structural equation modeling (PLS-SEM).FindingsResults confirm the relevance of emotional and social motivations to enjoy a CFTE and the need to align the target tourists' self-concept and restaurant positioning. The most vital driver of the CFTE is the restaurant innovativeness, which suggests that entrepreneurship in gastronomy is critical to boosting food tourism.Originality/valueThis study expands the understanding of the role of food tourism motivations and self-congruity in the tourist experience and underlines the relevance of the restaurant's production process to create authentic and compelling experiences that improve the tourists' satisfaction. Moderation analysis considering the tourists' previous experience reveals that cultural motivations only exert a positive effect on the CFTE for first-time travelers. However, self-congruity is the strongest predictor of a CFTE for repeat travelers.
Contextual antecedents of job crafting: review and future research agenda
Authors: Park Sunyoung , Park Sohee 
Published: October 2021

Purpose
The purpose of this study is to critically review current studies on job crafting to identify contextual factors related to employees’ job crafting and to integrate the findings to help organizations improve employees’ job crafting.

Design/methodology/approach
Based on the guidelines provided by Torraco (2016), the authors reviewed 44 quantitative studies on job crafting published between 2001and 2020.

Findings
The authors identified 35 contextual antecedents of job crafting at the job (11), group (6), leadership (12) and organizational (6) levels. The findings reveal that a significant number of studies have focused on contextual aspects related to employees’ job crafting. In particular, multiple studies discussed the important role of empowering leadership, servant leadership, transformational leadership, leader-member exchange in job crafting.

Originality/value
The authors emphasize contextual factors influencing job crafting including job, group, leadership and organizational levels. Based on the review, the authors suggest a future research agenda on job crafting in terms of job, group, leadership and organizational antecedents.
Logistics service providers' energy efficiency initiatives for environmental sustainability
Authors: Wehner Jessica , Taghavi Nejad Deilami Naghmeh , Altuntas Vural Ceren , Halldórsson Árni 
Published: October 2021

PurposeThis paper discusses logistics service providers' (LSPs’) energy efficiency initiatives for sustainable development, both from an evolutionary perspective and based on a framework consisting of actions, processes (i.e. at the operations interface) and services (i.e. at the customer interface).Design/methodology/approachFollowing a qualitative research design, semi-structured interviews were conducted with sustainability managers at LSPs and the data were analysed via inductive coding. Based on the results and the literature, the authors developed a maturity model for LSPs' transitions to environmental sustainability.FindingsLSPs' sustainable development occurs via operational processes, services at the customer interface, and actions that support those processes and services. Energy efficiency efforts are characterised by process depth that helps LSPs to align with their customers' energy efficiency improvement processes. While services related to energy efficiency connect LSPs and their customers, actions in support vary depending on the logistics activities in which LSPs participate.Research limitations/implicationsFurther research is needed to test and verify the maturity model and to clarify the interdependency of its three dimensions.Practical implicationsBy categorising energy efficiency initiatives and proposing a maturity model for LSPs' sustainable development via energy efficiency, the authors have developed a tool for logistics actors to assess their progress towards improved sustainability.Originality/valueThe paper contributes to the literature by providing a three-pillar framework to understand the sustainability transitions of LSPs through energy efficiency. Developing a maturity model using this framework also contributes to the literature with an approach to assess sustainability advancement in the logistics industry.
Female directors’ monitoring and earnings quality: the moderating effect of political connections and family ownership
Authors: Tee Chwee Ming , Kasipillai Jeyapalan 
Published: October 2021

PurposeThis study aims to examine whether the monitoring effectiveness of female directors in corporate boards is moderated by political connections and family ownership.Design/methodology/approachThis study employs a Malaysian dataset of listed firms from 2005 to 2015. The ordinary least squares model (OLS) is used to test all the research questions.FindingsThe authors find that female directors' monitoring is associated with higher earnings quality. This implies effective monitoring by female board directors. However, the monitoring effectiveness by female directors is attenuated by political connections; consistent with the argument that politically connected firms (PCFs) are plagued by severe agency problems. On the other hand, the association between female directors and higher earnings quality is strengthened by family ownership, indicating less severe type 1 agency problem.Originality/valueThis study is the first to show that the monitoring capability of female board directors is significantly influenced by a country's institutional setting. Although prior studies find that higher proportion of female directors is associated with higher earnings quality; the findings suggest that institutional setting such as political connections (family ownership) attenuate (strengthen) the monitoring effectiveness of female directors in the corporate board.
Hide and seek – the role of personality, sense of coherence and experiential information in hidden information needs
Authors: Heinström Jannica , Nikou Shahrokh , Sormunen Eero 
Published: October 2021

PurposeThe aim of this study is to assess the impact of personality traits and sense of coherence (SOC) on concealing information needs out of shame. The study also investigates the link between concealed information needs and the use of experiential information for psychological wellbeing.Design/methodology/approachA Partial Least Square ‐ Structural Equation Modelling (PLS-SEM) approach is used to assess and analyse the proposed conceptual model, which is based on the responses of 412 upper secondary school students.FindingsThe findings reveal that personality traits not only have direct significant effects on concealing information needs but that their effects are also mediated by SOC. The positive relationship between concealed information needs and the use of experiential information is confirmed in the study.Originality/valueThis study is the first to show that personality and SOC influence concealing information needs. Two pathways are found. Firstly, negative emotionality and a low SOC lead to a heightened sense of shame. Secondly, introversion induces a more guarded behaviour. The study, moreover, quantitatively demonstrates a link between concealed information needs and the use of experiential information for psychological wellbeing.
Firm age, sales growth and survival of internationalized SMEs: does human capital play a role?
Authors: Santhosh Channappa 
Published: October 2021

PurposeThis paper aims to explore the moderating effect of human capital in the form of a CEO’s educational background and firm age at the time of internationalization on growth and survival.Design/methodology/approachThe research study is based on primary data gathered from 102 internationalized small and medium enterprises (SMEs) belonging to the engineering industry in Bangalore district, Karnataka, India.FindingsThe results reveal that human capital significantly improves sales growth but had no impact on the survival of internationalized SMEs.Practical implicationsThe paper includes practical implications for the CEOs of SMEs to successfully strategize their efforts towards growth and survival in the international market.Originality/valueThis research study enhances the importance of human capital and its impact on the growth and survival of internationalized SMEs in the context of an emerging economy where research studies are limited and largely unexplored till date.
Governance thresholds and the human capital–growth nexus
Authors: Apergis Nicholas , Mustafa Ghulam , Khan Muhammad 
Published: October 2021

PurposeThe literature that explores the relationship between human capital and economic growth has produced mixed results. It highlights the puzzle on the correlations between human capital and economic growth. This study contributes to this debate by offering an explanation of the puzzling effects.Design/methodology/approachUsing the threshold model proposed by Kremer et al. (2013), the results document that there is a threshold effect in the human capital–growth nexus.FindingsThe findings illustrate that the relationship between human capital and economic growth is weakly positive up to a certain threshold level of governance; however, the relationship turns out to be positive once the threshold level has been achieved.Originality/valueThe mixed evidence on the human capital–growth relationship can be explained through institutional quality differences. The findings recommend that better governance is complementary to contribute to the productive use of human capital in achieving higher economic growth.
Real exchange rate synchronization in the NAFTA region
Authors: Baghestani Hamid 
Published: October 2021

PurposeThe literature mostly investigates the impact of trade and financial integration on business cycle synchronization. The author differs by focusing on the real effective exchange rate as the target variable in the North American Free Trade Agreement (NAFTA) region. In particular, the author investigates synchronization by analyzing the short- and long-run dynamics of the real effective exchange rates of Canada, Mexico and the US for 2008–2019.Design/methodology/approachThe author first employs stationarity and cointegration tests to specify and estimate the long-run equilibrium relation between the real effective exchange rates of Canada, Mexico and the US. The author then specifies and estimates an error-correction model for each real effective exchange rate in order to investigate whether the adjustment in eliminating disequilibrium is asymmetric.FindingsThe results indicate that the real effective exchange rates of Canada, Mexico and the US are cointegrated with only one long-run equilibrium relation. Canada's real effective exchange rate responds symmetrically to eliminate both negative and positive disequilibrium with a similar speed of adjustment. However, the response of Mexico's real effective exchange rate is asymmetric, as it responds to eliminate only positive disequilibrium. The US real effective exchange rate does not respond to disequilibrium, perhaps because it has a large economy with much stronger competition beyond the NAFTA region than both Canada and Mexico.Originality/valueThis is the first study that investigates real effective exchange rate synchronization in the NAFTA region.
The relationship between organizational culture, organizational silence and job performance in hotels: the case of Kuşadasi
Authors: Gencer Gullu , Atay Hakan , Gurdogan Arzu , Colakoglu Ulker 
Published: October 2021

PurposeThis study aims to measure the effect of organizational culture perceptions of hotel employees on their organizational silence behavior and job performance, as well as the effect of their organizational silence behavior on their job performance.Design/methodology/approachA correlational survey model was used in this research and a questionnaire was distributed to collect the data from 389 sampled employees working in four- and five-star hotels in the Kusadasi region in Turkey.FindingsIt was found that organizational culture was not significantly related to organizational silence but that organizational culture and its dimensions were significantly related to job performance. It was also revealed that while organizational silence was not significantly related to job performance, its dimensions were significantly related to job performance.Practical implicationsThe results of this study provide insight into organizational culture as an important factor in increasing job performance. The study also revealed how organizational silence behavior and its dimensions affect job performance. In this sense, accommodation establishments will be able to acquire new perspectives in terms of improving job performance.Originality/valueThis paper is deemed important, as it examined these three terms in one model in the field of tourism management. It is thought that it will contribute to the literature by closing the gap in the tourism literature while leading the way for future studies.
Employee perceptions of corporate hypocrisy and knowledge hiding: roles of moral identity and organization-based self-esteem
Authors: Zhao Hongdan , Liu Weiwei 
Published: October 2021

PurposeThis paper aims to investigate a new predictor of knowledge hiding, namely, employee perceptions of corporate hypocrisy (PCH). Based on the social cognitive theory, this study constructs a moderated mediation model linking PCH and knowledge hiding. The theoretical model concentrates on the mediating role of moral identity and the moderating role of organization-based self-esteem (OBSE).Design/methodology/approachTwo studies with different samples and designs were used. In Study 1, the experimental method explored whether PCH could elicit knowledge hiding under the condition of OBSE (H1 and H4). Study 2, an empirical method with three stages, tested the full mediated moderation model by adding to the mediating role of moral identity (H1–H4).FindingsThe results showed: PCH was positively related to knowledge hiding, moral identity mediated the influence of PCH on knowledge hiding and OBSE not only moderated the relationship between PCH and moral identity but also moderated the indirect effect of PCH on knowledge hiding (via moral identity). The present research sheds valuable light on the processes (how) and contingencies (when) whereby PCH affects knowledge hiding for the first time, thus extending prior research and encouraging further explorations on the topic of PCH and knowledge hiding. It informs practitioners that taking measures to decrease corporate hypocrisy plays a vital role in preventing workers from hiding knowledge.Originality/valueThe study’s distinctive contribution is to examine the mediating effect of moral identity and the moderating role of OBSE on the relationship between corporate hypocrisy and knowledge hiding, which through the lens of social cognitive theory. Thus, it furthers a deeper understanding of knowledge hiding and helps the organization understand the dynamics of knowledge management, such as prohibiting employee counterproductive behaviors in the workplace.
Defensive reactions and strategies for managing paradoxes of identity: three contrasting case studies
Authors: Ghares Mariem , Gilson Adeline , Ndiaye Adama 
Published: October 2021

PurposeIn fact, this research seeks to understand, on the one hand, the nature of defensive reactions and, on the other hand, the strategies mobilised by actors to deal with paradoxes of identity.Design/methodology/approachThe authors mobilise a qualitative methodology based on three contrasting longitudinal case studies, several sequences of non-participant observations, semi-structured interviews and secondary data.FindingsThe results show, on the one hand, close professional identities despite different organisations and, on the other hand, different reactions and strategies for managing paradoxes of identity despite the proximity of professional identities.Originality/valueBeyond the singularity of the paradoxes of identity studied, the research invites the authors to develop spaces for discussion.