bluuebunny/crossref_metadata_2025_split huggingface dataset:
A simple and rapid diagnostic method for 13 types of high-risk human papillomavirus (HR-HPV) detection using CRISPR-Cas12a technology
Authors: Gong Jiaojiao , Zhang Guanghui , Wang Wangguo , Liang Liping , Li Qianyun , Liu Menghao , Xue Liang , Tang Guanghui 
Published: June 2021

AbstractCervical carcinoma is the second most common cancer in women worldwide with greater than 99% of the cases caused by human papillomaviruses (HPVs). Early detection of HPVs especially the high risk types (HR-HPVs) are essential to prevent the disease progression. The existing methods for HPV detection, such as qPCR are of high sensitivity and specificity, but the need for expensive machinery and well-trained personnel slow down the disease detection. The emerging Cas12a-based method presents a new technique for nucleic acid detection. However, it is time-consuming and labor-intensive when used for HPV detection, as several reactions are required in order to identify multiple HPV infections. We herein present a non-genotyping method for 13 types of HR-HPV detection in a single reaction by combining the isothermal recombinase polymerase amplification (RPA) method with CRISPR-Cas12a technology. The result could be achieved in 35 min with high sensitivity (500 copies per reaction). This assay represents great advances for the application of RPA-Cas12a system and holds a great potential to address the key challenges facing the HPV diagnostics.
Ancient origin of fucosylated xyloglucan in charophycean green algae
Authors: Mikkelsen Maria Dalgaard , Harholt Jesper , Westereng Bjørge , Domozych David , Fry Stephen C. , Johansen Ida Elisabeth , Fangel Jonatan U. , Łężyk Mateusz , Feng Tao , Nancke Louise , Mikkelsen Jørn D. , Willats William G. T. , Ulvskov Peter 
Published: June 2021

AbstractThe charophycean green algae (CGA or basal streptophytes) are of particular evolutionary significance because their ancestors gave rise to land plants. One outstanding feature of these algae is that their cell walls exhibit remarkable similarities to those of land plants. Xyloglucan (XyG) is a major structural component of the cell walls of most land plants and was originally thought to be absent in CGA. This study presents evidence that XyG evolved in the CGA. This is based on a) the identification of orthologs of the genetic machinery to produce XyG, b) the identification of XyG in a range of CGA and, c) the structural elucidation of XyG, including uronic acid-containing XyG, in selected CGA. Most notably, XyG fucosylation, a feature considered as a late evolutionary elaboration of the basic XyG structure and orthologs to the corresponding biosynthetic enzymes are shown to be present inMesotaenium caldariorum.
Glucose regulates expression of pro-inflammatory genes, IL-1β and IL-12, through a mechanism involving hexosamine biosynthesis pathway-dependent regulation of α-E catenin
Authors: Dissanayake Waruni C. , Oh Jin Kyo , Sorrenson Brie , Shepherd Peter R. 
Published: June 2021

Abstract
High glucose levels are associated with changes in macrophage polarisation and evidence indicates that the sustained or even short-term high glucose levels modulate inflammatory responses in macrophages. However, the mechanism by which macrophages can sense the changes in glucose levels are not clearly understood. We find that high glucose levels rapidly increase the α-E catenin protein level in RAW264.7 macrophages. We also find an attenuation of glucose-induced increase in α-E catenin when hexosamine biosynthesis (HB) pathway is inhibited either with glutamine depletion or with the drugs azaserine and tunicamycin. This indicates the involvement of HB pathway in this process. Then, we investigated the potential role of α-E catenin in glucose-induced macrophage polarisation. We find that the reduction in α-E catenin level using siRNA attenuates the glucose-induced changes of both IL-1β and IL-12 mRNA levels under LPS-stimulated condition but does not affect TNF-α expression. Together this indicates that α-E catenin can sense the changes in glucose levels in macrophages via HB pathway and also can modulate the glucose-induced gene expression of inflammatory markers such as IL-1β and IL-12. This identifies a new part of the mechanism by which macrophages are able to respond to changes in glucose levels.
Exploring Current Practice, Knowledge, and Training Needs for Managing Psychosocial Concerns in the Audiology Setting: Perspectives of Audiologists, Audiology Reception Staff, and Managers
Authors: Bennett Rebecca J. , Kelsall-Foreman India , Donaldson Sara , Olaithe Michelle , Saulsman Lisa , Badcock Johanna C. 
Published: June 2021

Purpose
The purpose of this study was to explore the current practices and training requirements for supporting clients experiencing psychosocial concerns in the audiology setting, from the perspectives of audiology clinicians, managers, and reception staff.

Method

Convenience sampling was used to recruit audiologists, reception staff, and clinic managers (
N
= 13,
M
age
= 32.2 ± 8.1, range: 25–47 years, 11 female) through a large hearing services provider in Western Australia. A semistructured focus group was used to elicit participant views regarding current experiences relating to clients who express psychosocial concerns in the audiology setting, familiarity with psychosocial interventions, and training requirements for delivery of psychosocial interventions in the audiological setting.

Results
Twenty-four subthemes were identified across six themes: (1) awareness of psychosocial well-being, (2) the role of others, (3) identifying client's psychosocial needs, (4) managing client's psychosocial needs, (5) barriers to providing psychosocial support, and (6) broadening audiological services to include psychosocial support.

Conclusions
Participants reported an awareness of their clients' psychosocial challenges within the audiology setting, yet they described uncertainty in how best to respond in providing support and whether this was within their scope of practice. A majority of audiology staff expressed desire and motivation to broaden the scope of their service in order to better address their clients' hearing loss–related psychosocial needs.
Proposal of an integrated platoon‐based Round‐Robin algorithm with priorities for intersections with mixed traffic flows
Authors: Moradi Hossein , Sasaninejad Sara , Wittevrongel Sabine , Walraevens Joris 
Published: June 2021

AbstractThe growth of vehicle ownership has necessitated the adoption of new approaches to cope with the arising problems. In this regard, while technological advancement in connected and automated vehicles (CAVs) leads to a new source of information upon which effective intersection control mechanisms can be built, it also necessitates considering the issue of mixed traffic, on the one hand, and enhancing current infrastructures, on the other. This paper first proposes a framework, which enhances pre‐timed signals to incorporate CAVs and wireless communications. In this framework, Round‐Robin has been selected as the main algorithm treating vehicles' platoons as the units. Dealing with mixed traffic conditions and embedding an algorithm for prioritization of special vehicles are also discussed in the context of this framework. The result is a proposal of the Platoon‐based Round‐Robin algorithm with Priorities (the PRRP‐framework). This framework is further integrated into a speed advisory system to mutually augment each other's functions to provide for as much continuous movement of mixed traffic as possible. Performance indices obtained from the corresponding simulations show that while the PRRP‐framework is promising even with a low proportion of the CAVs, it is possible to get out considerably more benefits in the case of the proposed integration.
Design and analysis of a novel claw‐shaped modular stator relieving‐DC‐saturation doubly salient machine with 3D complementary magnetic circuit
Authors: Li Zhenghao , Niu Shuangxia 
Published: June 2021

AbstractHere, a novel DC‐excited doubly salient machine with a claw‐shaped modular stator and three‐dimensional (3D) complementary magnetic circuit is proposed for wind power generation application. The key is to artificially construct a 3D complementary structure to suppress torque ripple and employ auxiliary slot‐opening PMs to relieve DC‐saturation in the stator core and improve overload capability. The novel 3D complementary structure provides an alternative magnetic path for the main flux, so the flux leakages in the air gap at open‐circuit position are effectively cancelled, and the flux in the yoke remains relatively constant. Hence, the output torque ripple is suppressed. Furthermore, the saturation issue in the stator is solved with auxiliary permanent magnets which have opposite magnetization direction to that of DC field excitation. Therefore, the torque capacity of the machine is improved. Here, the machine structure and operation principle are introduced, and a 2D equivalent model is also developed to replace the 3D model to improve the calculation efficiency. Multi‐objective optimization algorithm is developed to improve the performance of this new machine. The validity of machine design is finally verified with 2D and 3D finite element analysis.
A vision of flexible dispatchable hybrid solar‐wind‐energy storage power plant
Authors: Bakhtvar Mostafa , Al Hinai Amer , El Moursi Mohamed S. , Albadi Mohammed H. 
Published: June 2021

AbstractDispatchable renewable generation is essential for 100% renewables power system. It is in the interest of both power system operators and customers since it reduces the need for flexibility and reserve. To this end, battery energy storage systems (BESS) are proposed for integration in the renewable power plant. This paper presents the optimal dispatch unit for a dispatchable hybrid solar‐wind power plant with BESS framework. It achieves optimal dispatchable renewable generation (from dispatchable hybrid renewable (solar‐wind) power plant with BESS, DHRB, operator perspective), subject to operational limits, by exploiting the synergy of wind and solar energy and combining it with storage capability of BESS using two different operation strategies, maximisation of revenues and maximisation of renewables harvesting. A continuous BESS degradation model is incorporated in the proposed rolling‐algorithm‐based‐optimal dispatch unit to improve the accuracy of results. The applicability of the proposed methodology and the performance of the operation strategies are demonstrated using a case study and the operation strategies are compared. Further, the effect of BESS size on its degradation and dispatchable power in a hybrid solar‐wind power plant with BESS are investigated through BESS lifetime. An indicative economic analysis is carried out to provide ground for the extra investment on and sizing of BESS for a dispatchable hybrid renewable power plant.
Variable structure interacting multiple model for highly aperiodic sensors with poor measurement precision
Authors: Lukeš Petr , Říha Milan 
Published: June 2021

AbstractThe issue of aircraft tracking using passive sensors with poor measurement precision and a variable update rate is investigated. First, the need for a time‐dependent Markov chain transition matrix for the Interacting Multiple Model (IMM) is thoroughly evaluated using simulation and real data from an experimental multilateration system. Three different methods for computing the time‐dependent matrix are compared, showing their superiority for highly aperiodic sensors. Second, the Variable Structure Interacting Multiple Model (VS‐IMM) for passive sensors with poor geometry is proposed, in which the mode set update is based on local dilution of precision or distance root mean square error. The performance of the estimator is compared with the IMM using real data. The VS‐IMM results in the best performance for areas of both high and low measurement precision.
Prophylactic colectomy and rectal preservation in FAP: systematic endoscopic follow-up and adenoma destruction changes natural history of polyposis
Authors: Pasquer Arnaud , Benech Nicolas , Pioche Mathieu , Breton Antoine , Rivory Jerome , Vinet Olivier , Poncet Gilles , Saurin Jean Christophe 
Published: June 2021

Abstract
Background and study aims Prophylactic surgery of familial adenomatous polyposis (FAP) includes total colectomy with ileorectal anastomosis (IRA) to proctocolectomy with ileoanal anastomosis (IAA). Surgical guidelines rely on studies without systematic endoscopic follow-up and treatment. Our aim was to report our experience based on a different approach: therapeutic follow-up, comparing in this setting IRA and IAA in terms of oncological safety and quality of life.
Patients and methods Between January 1965 and November 2015, all patients who underwent prophylactic surgery for FAP with therapeutic endoscopic follow-up in Lyon University hospital: systematic endoscopic treatment of adenomas, were retrospectively and prospectively (since 2011) included.
Results A total of 296 patients were analyzed: 92 had proctocolectomy with IAA (31.1 %), 197 total colectomy with IRA (66.5 %), and seven abdominoperineal resections (2.4 %). Median follow-up was 17.1 years (range, 0–38.1). Incidence of secondary cancer (IR vs. IAA) was 6.1 % vs. 1.1 % (P = 0.06; 95 %CI 0.001–0.36). The 15-year cancer-free and overall survival (IR vs. IAA) were 99.5 % vs 100 % (P = 0.09) and 98.9 % vs. 98.8 % (P = 0.82), respectively. Postoperative morbidity occurred in 44 patients: 29 (14.7 %) in the IRA and 15 (16.3 %) in the IAA group (P = 0.72). The mean number of stools per day in the respective groups were 4.4 (2.5) vs. 5.5 (2.6) (P = 0.001). Fecal incontinence occurred in 14 patients (7.1 %) in the IRA vs. 16 (17.4 %) in the IAA group (P = 0.03).
Conclusions A combination of therapeutic endoscopic treatment and extended rectal preservation appears to be a safe alternative to ileoanal J-pouch anastomosis.
Effect of DDAVP on Platelet Activation and Platelet-Derived Microparticle Generation
Authors: Persyn Matthieu , Athanase Nicolas , Trossaërt Marc , Sigaud Marianne , Ternisien Catherine , Béné Marie C. , Fouassier Marc 
Published: June 2021

Abstract
Background The way by which 1-deamino-8-D-arginine vasopressin (DDAVP) acts on platelets remains unclear. Data from the literature tend to show that there is no definite effect on platelet activation, but recent work has suggested that a subtype of platelets, activated by the combined action of collagen and thrombin, was triggered by DDAVP. Moreover, platelet microparticles (PMPs), which have been shown to be procoagulant, have rarely been studied in this context. The goal of this study was to analyze the effects of DDAVP on PMPs' release through platelet activation.
Methods Fifteen out of 18 consecutive patients undergoing a therapeutic test with DDAVP were included. They were suffering from factor VIII deficiency or from von Willebrand disease. The expression of P-selectin and PAC-1 binding on platelets and the numbers of circulating PMPs were evaluated ex vivo before and after DDAVP infusion. Peripheral blood was collected on CTAD to limit artifactual platelet activation.
Results DDAVP induced a significant decrease of platelet counts and volume. Only small changes of P-selectin expression and PAC-1 binding were observed. Considering PMPs, two populations of patients could be defined, respectively, with (120%, n = 6) or without (21%, n = 7) an increase of PMPs after DDAVP. The decrease in platelet counts and volume remained significant in the group of responders.
Conclusion This study shows that DDAVP induces the generation/release of PMPs in some patients with factor VIII deficiency and von Willebrand disease 1 hour after DDAVP infusion.
Management of early gastric cancer meeting criteria for endoscopic resection: US population-based study
Authors: Barakat Mohamed , Ramai Daryl , Cheung Derrick , Abdelfatah Mohamed M. , Othman Mohamed O. , Carr-Locke David L. , Adler Douglas G. 
Published: June 2021

Abstract
Background and study aims The goal of this study was to assess surgical resection (SR) of early gastric cancer (EGC) fitting Japanese Gastric Cancer Association (JGCA) endoscopic resection (ER) criteria.
Patients and methods We analyzed EGC data from the national Surveillance, Epidemiology, and End Results (SEER) database from 2010 to 2015.
Results A total of 2219 EGC cases were identified (1074 T1a and 1145 T1b). Of them, 409 met absolute criteria, 219 met expanded 1, 529 expanded 2, and 229 expanded 3. 259 lesions were treated endoscopically while 1007 were surgically resected (20.5 % vs 79.5 %, P = 0.0001). Temporal analysis showed that the frequency of ER steadily increased while SR proportionally decreased during the study period. Cox proportion regression analysis adjusting for confound variables (including age, gender, and race) showed no significant difference in the risk of mortality following either surgery or endoscopy.
Conclusions EGC can be safely treated with ER. However, EGC meeting JGCA ER criteria is largely treated with SR in the United States.
Homoeopathic Management of Conduct Disorder: A Case Series
Authors: Moorthi S. Karunakara , Radhika P. 
Published: June 2021

AbstractConduct disorder is a psychiatric syndrome occurring in childhood and adolescence, and is characterised by a long-standing pattern of violations of rules and antisocial behaviour. The aetiology of conduct disorder involves an interaction of genetic/constitutional, familial and social factors. Conduct disorder increases the risk of several public health problems, including violence, weapon use, substance abuse and dropping out of school. Thus, it is important to identify conduct disorder and begin intervention as early as possible.Three cases of conduct disorder are presented here, which have been treated exclusively with homoeopathic medicines without any supportive therapies. The diagnosis and severity were assessed by Conduct Disorder Diagnostic Tool and the symptomatic assessment was done by using Conduct Disorder Rating Scale-Parent Version. Possible causal attribution of changes was explicitly depicted by Naranjo Criteria.The initial outcome of homoeopathic treatment shows a positive role of homoeopathy as the cases are exclusively managed by homoeopathic medicine.
Dietary Treatment for NAFLD: New Clinical and Epidemiological Evidence and Updated Recommendations
Authors: Zelber-Sagi Shira 
Published: June 2021

AbstractThe key factor in preventing and treating nonalcoholic fatty liver disease (NAFLD) is a holistic lifestyle modification approach, encompassing diet based on healthy eating patterns of unprocessed foods, exercise, balanced drinking, and smoking habits. The Mediterranean diet and other healthy dietary patterns can reduce liver fat and may be related with lower disease progression. The type of diet should be tailored to the patient's cultural and personal preferences. Changing dietary composition without reducing caloric intake may offer an additional and sometimes more feasible alternative, so that the nutritional treatment incorporates, but is not focused on, weight reduction goals. The growing global consumption of ultra-processed foods, which is the polar opposite of the Mediterranean diet and its concept of home-based cooking, poses a great challenge in the prevention of NAFLD and probably hepatocellular carcinoma.This review will cover the most updated clinical and epidemiological evidence for lifestyle treatment in NAFLD and provide practical treatment tools.
Beyond undocumented: Differences in the mental health of Latinx undocumented college students
Authors: Flores Morales Josefina , Garcia Yuliana 
Published: June 2021

AbstractUndocumented college students face several threats to their well-being and mental health. Different social locations, including whether students have Deferred Action for Childhood Arrival (DACA) status, students’ gender, and family factors may shape students’ ability to be well. How these factors work together to shape mental health outcomes among undocumented Latinx college students is not well understood. This study examines several factors (demographic, familial, immigration, and socioeconomic factors) associated with anxiety scores of undocumented Latinx college students who participated in the UndocuScholars Project national online survey in 2014. We observe three notable findings: (1) DACA recipients report heightened levels of anxiety, (2) women with DACA status report higher levels of anxiety compared to non-DACAmented undocumented college students and men with DACA, and (3) students whose families motivate them report lower levels of anxiety. Latinx undocumented college students are not a monolith; demographic, family, and socioeconomic factors matter.
Deep learning combined with IAST to screen thermodynamically feasible MOFs for adsorption-based separation of multiple binary mixtures
Authors: Anderson Ryther , Gómez-Gualdrón Diego A. 
Published: June 2021

The structures of metal–organic frameworks (MOFs) can be tuned to reproducibly create adsorption properties that enable the use of these materials in fixed-adsorption beds for non-thermal separations. However, with millions of possible MOF structures, the challenge is to find the MOF with the best adsorption properties to separate a given mixture. Thus, computational, rather than experimental, screening is necessary to identify promising MOF structures that merit further examination, a process traditionally done using molecular simulation. However, even molecular simulation can become intractable when screening an expansive MOF database for their separation properties at more than a few composition, temperature, and pressure combinations. Here, we illustrate progress toward an alternative computational framework that can efficiently identify the highest-performing MOFs for separating various gas mixtures at a variety of conditions and at a fraction of the computational cost of molecular simulation. This framework uses a “multipurpose” multilayer perceptron (MLP) model that can predict single component adsorption of various small adsorbates, which, upon coupling with ideal adsorbed solution theory (IAST), can predict binary adsorption for mixtures such as Xe/Kr, CH4/CH6, N2/CH4, and Ar/Kr at multiple compositions and pressures. For this MLP+IAST framework to work with sufficient accuracy, we found it critical for the MLP to make accurate predictions at low pressures (0.01–0.1 bar). After training a model with this capability, we found that MOFs in the 95th and 90th percentiles of separation performance determined from MLP+IAST calculations were 65% and 87%, respectively, the same as MOFs in the simulation-predicted 95th percentile across several mixtures at diverse conditions (on average). After validating our MLP+IAST framework, we used a clustering algorithm to identify “privileged” MOFs that are high performing for multiple separations at multiple conditions. As an example, we focused on MOFs that were high performing for the industrially relevant separations 80/20 Xe/Kr at 1 bar and 80/20 N2/CH4 at 5 bars. Finally, we used the MOF free energies (calculated on our entire database) to identify privileged MOFs that were also likely synthetically accessible, at least from a thermodynamic perspective.
Naming unrelated words predicts creativity
Authors: Olson Jay A. , Nahas Johnny , Chmoulevitch Denis , Cropper Simon J. , Webb Margaret E. 
Published: June 2021

Significance
Many traditional measures of creativity require time-intensive and subjective scoring procedures. Their scores are relative to the specific sample, which makes multicultural or international assessments difficult. Our results show that a shorter and simpler task with automatic and objective scoring may be at least as reliable at measuring verbal creativity. This finding enables assessments across larger and more diverse samples with less bias.