bluuebunny/crossref_metadata_2025_split huggingface dataset:
Dosimetric quantities and effective dose in medical imaging: a summary for medical doctors
Authors: Vano Eliseo , Frija Guy , Loose Reinhard , Paulo Graciano , Efstathopoulos Efstathios , Granata Claudio , Andersson Jonas ,   the European Society of Radiology (ESR)
Published: July 2021

AbstractThis review presents basic information on the dosimetric quantities used in medical imaging for reporting patient doses and establishing diagnostic reference levels. The proper use of the radiation protection quantity “effective dose” to compare doses delivered by different radiological procedures and different imaging modalities with its uncertainties and limitations, is summarised. The estimates of population doses required by the European Directive on Basic Safety Standards is commented on. Referrers and radiologists should be familiar with the dose quantities to inform patients about radiation risks and benefits. The application of effective dose on the cumulative doses from recurrent imaging procedures is also discussed. Patient summary: Basic information on the measurement units (dosimetric quantities) used in medical imaging for reporting radiation doses should be understandable to patients. The Working Group on “Dosimetry for imaging in clinical practice” recommended that a brief explanation on the used dosimetric quantities and units included in the examination imaging report, should be available for patients. The use of the quantity “effective dose” to compare doses to which patients are exposed to from different radiological procedures and its uncertainties and limitations, should also be explained in plain language. This is also relevant for the dialog on to the cumulative doses from recurrent imaging procedures. The paper summarises these concepts, including the need to estimate the population doses required by the European Directive on Basic Safety Standards. Referrers and radiologists should be familiar with the dose quantities to inform patients about radiation risks and benefits.
Development of cavitary lung disease as a long-term complication of coronavirus disease 2019 in a young previously healthy patient: a case report
Authors: Egoryan Goar , Hyser Elise , Mushtaq Ammar H. , Yanez-Bello Maria Adriana , Trelles-Garcia Daniela Patricia , Friedman Harvey J. , Rodriguez-Nava Guillermo 
Published: July 2021

Abstract
Background
Cavities are frequent manifestations of a wide variety of pathological processes involving the lung. There has been a growing body of evidence of coronavirus disease 2019 leading to a cavitary pulmonary disease.

Case presentation
A healthy 29-year-old Filipino male presented to the hospital a couple of months after convalescence from coronavirus disease 2019 with severe pleuritic chest pain, fever, chills, and shortness of breath, and was found to have a cavitary lung lesion on chest computed tomography. While conservative management alone failed to improve the patient’s condition, he ultimately underwent left lung video-assisted thoracoscopic surgery decortication. Even though the surgical pathology revealed only necrosis with dense acute inflammation and granulation tissue with no microorganisms, he gradually improved with medical therapy adjunct with surgical therapy.

Conclusion
Documented cases of cavitary lung disease secondary to coronavirus disease 2019 have been mostly reported in the acute or subacute phase of the infection. However, clinicians should recognize this entity as a late complication of coronavirus disease 2019, even in previously healthy individuals.
Exosome-transported circRNA_0001236 enhances chondrogenesis and suppress cartilage degradation via the miR-3677-3p/Sox9 axis
Authors: Mao Guping , Xu Yiyang , Long Dianbo , Sun Hong , Li Hongyi , Xin Ruobin , Zhang Ziji , Li Zhiwen , Yang Zhi , Kang Yan 
Published: July 2021

Abstract
Objectives
Aberrations in exosomal circular RNA (circRNA) expression have been identified in various human diseases. In this study, we investigated whether exosomal circRNAs could act as competing endogenous RNAs (ceRNAs) to regulate the pathological process of osteoarthritis (OA). This study aimed to elucidate the specific MSC-derived exosomal circRNAs responsible for MSC-mediated chondrogenic differentiation using human bone marrow-derived MSCs (hMSCs) and a destabilization of the medial meniscus (DMM) mouse model of OA.

Methods
Exosomal circRNA deep sequencing was performed to evaluate the expression of circRNAs in human bone marrow-derived MSCs (hMSCs) induced to undergo chondrogenesis from day 0 to day 21. The regulatory and functional roles of exosomal circRNA_0001236 were examined on day 21 after inducing chondrogenesis in hMSCs and were validated in vitro and in vivo. The downstream target of circRNA_0001236 was also explored in vitro and in vivo using bioinformatics analyses. A luciferase reporter assay was used to evaluate the interaction between circRNA_0001236 and miR-3677-3p as well as the target gene sex-determining region Y-box 9 (Sox9). The function and mechanism of exosomal circRNA_0001236 in OA were explored in the DMM mouse model.

Results
Upregulation of exosomal circRNA_0001236 enhanced the expression of Col2a1 and Sox9 but inhibited that of MMP13 in hMSCs induced to undergo chondrogenesis. Moreover, circRNA_0001236 acted as an miR-3677-3p sponge and functioned in human chondrocytes via targeting miR-3677-3p and Sox9. Intra-articular injection of exosomal circRNA_0001236 attenuated OA in the DMM mouse model.

Conclusions
Our results reveal an important role for a novel exosomal circRNA_0001236 in chondrogenic differentiation. Overexpression of exosomal circRNA_0001236 promoted cartilage-specific gene and protein expression through the miR-3677-3p/Sox9 axis. Thus, circRNA_0001236-overexpressing exosomes may alleviate cartilage degradation, suppressing OA progression and enhancing cartilage repair. Our findings provide a potentially effective therapeutic strategy for treating OA.
Exosomal miR-100-5p inhibits osteogenesis of hBMSCs and angiogenesis of HUVECs by suppressing the BMPR2/Smad1/5/9 signalling pathway
Authors: Yang Wu , Zhu Weiwen , Yang Yunfei , Guo Minkang , Qian Husun , Jiang Weiqian , Chen Yu , Lian Chengjie , Xu Zijie , Bai Haobo , Chen Tingmei , Zhang Jian 
Published: July 2021

Abstract
Background
Nontraumatic osteonecrosis of the femoral head (NONFH) is a common, progressive, and refractory orthopaedic disease. Decreased osteogenesis and angiogenesis are considered the main factors in the pathogenesis of NONFH. We aimed to figure out whether exosomes and exosomal miRNA from necrotic bone tissues of patients with NONFH are involved in the pathogenesis of NONFH and reveal the underlying mechanisms.

Methods
RT-PCR and western blotting (WB) were used to detect the expression of osteogenic, adipogenic, and angiogenic markers. ALP staining and Alizarin Red S (ARS) staining were used to evaluate osteogenic differentiation of human bone marrow-derived mesenchymal stem cells (hBMSCs). Oil Red O staining was performed to assess the adipocyte deposition. A tube formation assay was used to study angiogenesis of human umbilical vascular endothelial cells (HUVECs). H&E staining and immunohistochemistry (IHC) staining were used to detect the effect of the NONFH exosomes in vivo. MicroRNA sequencing was conducted to identify potential regulators in the NONFH exosomes. The target relationship between miR-100-5p and BMPR2 was predicted and confirmed by a dual luciferase reporter assay and WB.

Results
The NONFH exosomes reduced the osteogenic differentiation of hBMSCs and angiogenesis of HUVECs. In addition, the injection of the NONFH exosomes caused thinning and disruption of bone trabeculae in the femoral heads of rats. MiR-100-5p expression was upregulated in the NONFH exosomes and inhibited the osteogenesis of hBMSCs and angiogenesis of HUVECs by targeting BMPR2 and suppressing the BMPR2/SMAD1/5/9 signalling pathway. Silencing miR-100-5p expression rescued the reduction in osteogenesis and angiogenesis caused by the NONFH exosomes by activating the BMPR2/SMAD1/5/9 signalling pathway.

Conclusion
The NONFH exosomal miR-100-5p can lead to NONFH-like damage by targeting BMPR2 and suppressing the BMPR2/SMAD1/5/9 signalling pathway, which may be involved in the pathophysiological mechanisms of nontraumatic osteonecrosis of the femoral head (NONFH).
Cell heterogeneity, rather than the cell storage solution, affects the behavior of mesenchymal stem cells in vitro and in vivo
Authors: Wang Yong-Hong , Tao Ya-Chao , Wu Dong-Bo , Wang Meng-Lan , Tang Hong , Chen En-Qiang 
Published: July 2021

Abstract
Background
Mesenchymal stem cells (MSCs) have to be expanded in vitro to reach a sufficient cell dose for the treatment of various diseases. During the process of expansion, some obstacles remain to be overcome. The purpose of this study was to investigate the effects of storage solutions and heterogeneity on the behavior of MSCs in vitro and in vivo.

Methods
Umbilical cord MSCs (UC-MSCs) of similar sizes within normal ranges were suspended in three different storage solutions, phosphate buffer solution, normal saline, and Dulbecco’s modified Eagle medium. Then, the ultrastructure, viability, and safety of these cells were compared. Other two UC-MSC populations of different sizes were categorized based on their mean diameters. The ultrastructure, proliferation, immunosuppression, hepatic differentiation potential, and number of senescent cells were investigated and compared. The survival rates of mice after the infusion of UC-MSCs of different sizes were compared.

Results
For UC-MSCs suspended in different storage solutions, the cell apoptosis rates, ultrastructure, and survival rates of mice were similar, and no differences were observed. Cells with a diameter of 19.14 ± 4.89 μm were categorized as the larger UC-MSC population, and cells with a diameter of 15.58 ± 3.81 μm were categorized as the smaller population. The mean diameter of the larger UC-MSC population was significantly larger than that of the smaller UC-MSC population (p < 0.01). Smaller UC-MSCs had more powerful proliferation and immunosuppressive potential and a higher nucleus-cytoplasm ratio than those of large UC-MSCs. The number of cells positive for β-galactosidase staining was higher in the larger UC-MSC population than in the smaller UC-MSC population. The survival rates of mice receiving 1 × 106 or 2 × 106 smaller UC-MSCs were 100%, both of which were higher than those of mice receiving the same amounts of larger UC-MSCs (p < 0.01). The cause of mouse death was explored and it was found that some larger UC-MSCs accumulated in the pulmonary capillary in dead mice.

Conclusion
Different storage solutions showed no significant effects on cell behavior, whereas heterogeneity was quite prevalent in MSC populations and might limit cells application. Hence, it is necessary to establish a more precise standardization for culture-expanded MSCs.
High risk of patient self-inflicted lung injury in COVID-19 with frequently encountered spontaneous breathing patterns: a computational modelling study
Authors: Weaver Liam , Das Anup , Saffaran Sina , Yehya Nadir , Scott Timothy E. , Chikhani Marc , Laffey John G. , Hardman Jonathan G. , Camporota Luigi , Bates Declan G. 
Published: July 2021

AbstractBackgroundThere is on-going controversy regarding the potential for increased respiratory effort to generate patient self-inflicted lung injury (P-SILI) in spontaneously breathing patients with COVID-19 acute hypoxaemic respiratory failure. However, direct clinical evidence linking increased inspiratory effort to lung injury is scarce. We adapted a computational simulator of cardiopulmonary pathophysiology to quantify the mechanical forces that could lead to P-SILI at different levels of respiratory effort. In accordance with recent data, the simulator parameters were manually adjusted to generate a population of 10 patients that recapitulate clinical features exhibited by certain COVID-19 patients, i.e., severe hypoxaemia combined with relatively well-preserved lung mechanics, being treated with supplemental oxygen.ResultsSimulations were conducted at tidal volumes (VT) and respiratory rates (RR) of 7 ml/kg and 14 breaths/min (representing normal respiratory effort) and at VT/RR of 7/20, 7/30, 10/14, 10/20 and 10/30 ml/kg / breaths/min. While oxygenation improved with higher respiratory efforts, significant increases in multiple indicators of the potential for lung injury were observed at all higher VT/RR combinations tested. Pleural pressure swing increased from 12.0 ± 0.3 cmH2O at baseline to 33.8 ± 0.4 cmH2O at VT/RR of 7 ml/kg/30 breaths/min and to 46.2 ± 0.5 cmH2O at 10 ml/kg/30 breaths/min. Transpulmonary pressure swing increased from 4.7 ± 0.1 cmH2O at baseline to 17.9 ± 0.3 cmH2O at VT/RR of 7 ml/kg/30 breaths/min and to 24.2 ± 0.3 cmH2O at 10 ml/kg/30 breaths/min. Total lung strain increased from 0.29 ± 0.006 at baseline to 0.65 ± 0.016 at 10 ml/kg/30 breaths/min. Mechanical power increased from 1.6 ± 0.1 J/min at baseline to 12.9 ± 0.2 J/min at VT/RR of 7 ml/kg/30 breaths/min, and to 24.9 ± 0.3 J/min at 10 ml/kg/30 breaths/min. Driving pressure increased from 7.7 ± 0.2 cmH2O at baseline to 19.6 ± 0.2 cmH2O at VT/RR of 7 ml/kg/30 breaths/min, and to 26.9 ± 0.3 cmH2O at 10 ml/kg/30 breaths/min.ConclusionsOur results suggest that the forces generated by increased inspiratory effort commonly seen in COVID-19 acute hypoxaemic respiratory failure are comparable with those that have been associated with ventilator-induced lung injury during mechanical ventilation. Respiratory efforts in these patients should be carefully monitored and controlled to minimise the risk of lung injury.
Ostrowski-type inequalities for n-polynomial $\mathscr{P}$-convex function for k-fractional Hilfer–Katugampola derivative
Authors: Naz Samaira , Naeem Muhammad Nawaz , Chu Yu-Ming 
Published: July 2021

AbstractIn this article, we develop a novel framework to study a new class of convex functions known as n-polynomial $\mathscr{P} $
P
-convex functions. The purpose of this article is to establish a new generalization of Ostrowski-type integral inequalities by using a generalized k-fractional Hilfer–Katugampola derivative. We employ this technique by using the Hölder and power-mean integral inequalities. We present analogs of the Ostrowski-type integrals inequalities connected with the n-polynomial $\mathscr{P}$
P
-convex function. Some new exceptional cases from the main results are obtained, and some known results are recaptured. In the end, an application to special means is given as well. The article seeks to create an exciting combination of a convex function and special functions in fractional calculus. It is supposed that this investigation will provide new directions in fractional calculus.
What is the impact of distraction osteogenesis on the upper airway of hemifacial microsomia patient with obstructive sleep apnea: a case report
Authors: Wang Rongyang , Xu Shixing , Yang Ruimei 
Published: July 2021

Abstract
Background
Current research about hemifacial microsomia (HFM) patients after distraction osteogenesis (DO) most emphasize the morphologic changes. This case report shows the outcome of DO on the upper airway of a HFM patient with obstructive sleep apnea (OSA) based on the use of computational fluid dynamics (CFD).

Case presentation
An 11-year-old boy was diagnosed as HFM with OSA, and underwent unilateral DO. Polysomnography and CT scans were performed before and 6 months after treatment. After DO, lowest blood oxygen saturation increased from 81% to 95% and apnea and hypopnea index decreased from 6.4 events/hour to 1.2 events/hour. The oropharynx and nasopharynx were obviously expanded. We observed apparently increased average pressure, decreased average velocity and pressure drop in all cross-sections, and largely decreased airflow resistance and maximum velocity entirely in the airway.

Conclusions
The results suggest that DO might be effective for the treatment of OSA by expanding the upper airway and reducing the resistance of inspiration.
Feasibility of quantitative inorganic arsenic speciation at the parts-per-trillion level using solid phase extraction and femtosecond laser ablation inductively coupled plasma mass spectrometry
Authors: Lee Seon Hwa , Yang Seon-Jin , Lee Yonghoon , Nam Sang-Ho 
Published: July 2021

AbstractToxicity of arsenic compounds depends on the chemical structure as well as the concentration. Thus, separation of the toxic arsenic species should precede the quantification for the accurate toxicity assessment. Ion chromatography inductively coupled plasma mass spectrometry (IC-ICP-MS) has been the most popular method for separation and quantification of toxic arsenic species. However, the method requires complex instrument, elaborate sample preparation, and long analysis time. In this work, toxic inorganic arsenic species in water was separated by the simple solid phase extraction (SPE) using a strong anion-exchange membrane filter, and then the membrane filter was analyzed by femtosecond laser ablation inductively coupled plasma mass spectrometry (fs-LA-ICP-MS). The pH value of the sample was adjusted to 4 using ammonium hydroxide and phosphoric acid for the complete separation of the toxic inorganic arsenic from the other organic arsenics. The linear dynamic range was from 0.5 to 1000 μg/kg, and the correlation coefficient was 0.99989. The recovery efficiency was 96‑106%. The detection limit of the inorganic arsenic was 0.028 μg/kg. Our results indicate that SPE-fs-LA-ICP-MS provides enough analytical performance to analyze the toxic inorganic arsenic in water at the level of parts per trillion using the simple separation method and the rapid laser ablation sampling.
Prevalence of an angiotensin-converting enzyme gene variant in dogs
Authors: Adin D. B. , Atkins C. E. , Friedenberg S. G. , Stern J. A. , Meurs K. M. 
Published: July 2021

Abstract
Background
Genetic heterogeneity of the canine angiotensin converting enzyme (ACE) gene is functionally important because the degree of aldosterone breakthrough with ACE-inhibitor therapy is greater in variant positive dogs compared to variant negative dogs, but the prevalence of the variant is not known. The purpose of this study was to determine ACE gene variant-positive prevalence in a population of 497 dogs of different breeds.

Results
Overall variant-positive prevalence was 31%, with 20% of dogs heterozygous and 11% of dogs homozygous. The variant was overrepresented in Irish Wolfhounds (prevalence 95%; P < .001), Dachshunds (prevalence 90%; P < .001), Cavalier King Charles Spaniels (prevalence 85%; P < .001), Great Danes (prevalence 84%; P < .001), and Bull Mastiffs (prevalence 58%; P = .02). Irish Wolfhounds were more likely to be homozygous than heterozygous (P < .001).

Conclusions
Nearly one-third of dogs in this study were positive for a functionally important ACE gene variant, with wide prevalence variability between breeds. The clinical importance of high ACE gene variant-positive prevalence in some breeds requires further study because the highest prevalences were found in breeds that are predisposed to heart disease and therefore may be treated with ACE-inhibitors.
Evaluating deep learning EEG-based mental stress classification in adolescents with autism for breathing entrainment BCI
Authors: Sundaresan Avirath , Penchina Brian , Cheong Sean , Grace Victoria , Valero-Cabré Antoni , Martel Adrien 
Published: July 2021

AbstractMental stress is a major individual and societal burden and one of the main contributing factors that lead to pathologies such as depression, anxiety disorders, heart attacks, and strokes. Given that anxiety disorders are one of the most common comorbidities in youth with autism spectrum disorder (ASD), this population is particularly vulnerable to mental stress, severely limiting overall quality of life. To prevent this, early stress quantification with machine learning (ML) and effective anxiety mitigation with non-pharmacological interventions are essential. This study aims to investigate the feasibility of exploiting electroencephalography (EEG) signals for stress assessment by comparing several ML classifiers, namely support vector machine (SVM) and deep learning methods. We trained a total of eleven subject-dependent models-four with conventional brain-computer interface (BCI) methods and seven with deep learning approaches-on the EEG of neurotypical (n=5) and ASD (n=8) participants performing alternating blocks of mental arithmetic stress induction, guided and unguided breathing. Our results show that a multiclass two-layer LSTM RNN deep learning classifier is capable of identifying mental stress from ongoing EEG with an overall accuracy of 93.27%. Our study is the first to successfully apply an LSTM RNN classifier to identify stress states from EEG in both ASD and neurotypical adolescents, and offers promise for an EEG-based BCI for the real-time assessment and mitigation of mental stress through a closed-loop adaptation of respiration entrainment.
Impact of the blooming artefact on dental implant dimensions in 13 cone-beam computed tomography devices
Authors: Wanderley Victor Aquino , de Faria Vasconcelos Karla , Leite Andre Ferreira , Pauwels Ruben , Shujaat Sohaib , Jacobs Reinhilde , Oliveira Matheus L. 
Published: July 2021

Abstract
Background
The purpose of this study was to objectively assess dimensional alteration (blooming artefact) on dental implant using 13 cone-beam computed tomography (CBCT) devices adjusted to device-specific scanning protocols and to assess whether subjective adjustment of brightness and contrast (B&C) could alter its visualization.

Methods
An anthropomorphic phantom containing a dental implant was scanned in 13 CBCT devices adjusted to three scanning protocols: medium-FOV standard resolution, small-FOV standard resolution, and small-FOV high resolution. The diameter of the implant was measured at five levels, averaged, and compared with those from a reference standard industrial CT image. B&C adjustments were performed and measurements were repeated. The intraclass correlation coefficient assessed the reliability of the measurements and general linear mixed models were applied for multiples comparisons at a 95% confidence interval.

Results
Implant diameter obtained from small-FOV high-resolution protocols in most CBCT devices was not significantly different when compared to that from the reference (p > 0.05). For standard protocols, significant dimensional alteration of the implant ranging from 23 to 34% (0.67 to 1.02 mm) was observed in 9 CBCT devices for small-FOV scanning (p < 0.05), and in 8 CBCT devices for medium-FOV scanning, implant dimensional alteration ranged significantly from 21 to 35% (0.62 to 1.04 mm). After B&C adjustments, dimensional alteration was reduced for several of the CBCT devices tested (p < 0.05).

Conclusions
The visualization of the implant dimensional alteration differed between CBCT devices and scanning protocols with an increase in diameter ranging from 0.27 to 1.04 mm. For most CBCT devices, B&C adjustments allowed to reduce visualization of implant blooming.