bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effect of antibodies to agglutinogens 1 and 2, filamentous hemagglutinin and pertussis toxin on formation of Bordetella pertussis biofilms on abiotic substrate
Authors: Zaytsev E. M. , Britsina M. V. , Ozeretskovskaya M. N. , Mertsalova N. U. , Bazhanova I. G. 
Published: July 2021

Aim. Study of the effect of antibodies to agglutinogens 1 and 2, filamentous hemagglutinin (FHA) and pertussis toxin (PT) on the formation of biofilms by Bordetella pertussis strains on the abiotic substrate.Materials and methods. Vaccine-derived and freshly isolated strains of B. pertussis were used. Cultures of strains grown on a dense nutrient medium were used as an inoculum for obtaining biofilms. The intensity of biofilm formation in round-bottomed polystyrene 96-well plates in the presence of antisera to agglutinogens 1 and 2, antiserum to FHA, and monoclonal antibodies (MСА) to the S1, S2, and S3 subunits of PT was evaluated by staining with 0.1% gentian-violet solution.Results. Most of the studied strains were sensitive to antibodies, which was manifested in complete suppression of biofilm formation. All strains were sensitive to antiserum to agglutinogen 1, antiserum to FHA, and MCA to the S2 subunit of KT. Three out of 4 studied strains with this agglutinogen in their composition were sensitive to antiserum to agglutinogen 2: No. 475 (serotype 1.2.3), No. 317 (serotype 1.2.3) and No. 178 (serotype 1.2.0). Relative resistance to antiserum was detected only in serotype 1.2.0 strain No. 305, but with minimal dilution, the intensity of biofilm formation was 1.8 times lower than in the control group. Strains No. 703 (serotype 1.0.3) and No. 287 (serotype 1.0.3) that did not have agglutinogen 2 were resistant to antiserum. Four and 5 out of the 6 strains used were sensitive to the S1 and S3 subunits of PT, respectively. Strain No. 305 was resistant to MCA to the S1 and S3 subunits, and strain No. 287 to MCA to the S1 subunit. At the same time, the intensity of biofilm formation was 2 and 1.8 times lower than in the control at the minimum MCA dilution.Conclusion. These data indicate that the growth of biofilms of B. pertussis strains is suppressed by antibodies both to the surface structures of the microbial cell (agglutinogens 1 and 2, FHA) and to the S1, S2 and S3 subunits of PT.
Adaptation of the MTT assay for detection of neutralizing antibodies against the SARS-CoV-2 virus
Authors: Gracheva A. V. , Korchevaya E. R. , Kudryashova A. M. , Borisova O. V. , Petrusha O. A. , Smirnova D. I. , Chernyshova I. N. , Svitich O. A. , Zverev V. V. , Faizuloev E. B. 
Published: July 2021

Introduction. The ability of SARS-CoV-2 antibodies to neutralize the virus is the primary indicator of their specific activity. The test for virus neutralizing antibodies (NAbs) is much needed in different biomedical studies.The aim of the study is to find optimum conditions for microscopic and spectrophotometric detection of SARSCoV-2 NAbs by inhibition of cytopathic effect (CPE) in cell cultures.Materials and methods. Blood sera collected from COVID-19 convalescent patients and healthy individuals (n = 96) were tested using the ELISA method. The SARS-CoV-2 coronavirus, Dubrovka strain (GenBank accession no. MW514307.1) was grown in culture medium of Vero cell line CCL-81 (ATCC). Real-time RT-PCR, ELISA, and Sanger sequencing were used for detection of the virus. The results of the neutralization test (NT) were assessed through the microscopic examination for CPE and by the methyl thiazolyl tetrazolium (MTT) assay.Results. SARS-CoV-2 was isolated from a COVID-19 patient and adapted to grow in cell culture. At a low dose of infection (MOI = 0.00001), the virus caused a pronounced CPE with the cell viability less than 3%, thus making it possible to assess NT results by CPE inhibition. The NT and ELISA-based comparative study of sera showed positive correlation between virus NAb titers and Nab titers to S-protein RBD (Spearman’s r = 0.714; p 0.001). The results of NAbs microscopic and spectrophotometric detection (the MTT assay) also demonstrated positive correlation (Spearman’s r = 0.963; p 0.05).Conclusion. The SARS-CoV-2 virus adapted to Vero cell culture served to develop a NAb titer assessment system, which can be used both in microscopic studies and for an MTT assay in spectrophotometric studies. The MTT assay provides automated reading of NT results, optimizes the statistical analysis of the obtained data, and minimizes subjectivity in assessment of results. Being a vital dye, MTT penetrates only viable cells, thus contributing to the reliability of the obtained results compared to other dyes.
UPAYA PERBAIKAN WAKTU KERJA UNTUK MENINGKATKAN PRODUKTIVITAS KERJA ( STUDI KASUS DI PERUSAHAAN OTOMOTIF) KARAWANG
Authors: Ade Suhara  
Published: July 2021

Suatu perusahaan seringkali dihadapkan pada suatu permasalahan yang berkaitan dengan upaya pencapaian target produksi. Saat ini, perusahaansedang menitik beratkan pada proses perakitan, proses produksi yang dilakukannya masih sering dilakukan proses repair terhadap produk akhir. Proses repair tersebut tentunya akan mengakibatkan hilangnya waktu produksi dan keterlambatan pendistribusian produk.
Meningkatnya grafik produk reject merupakan suatu indikasi adanya kehilangan waktu kerjayang akan menyebabkan turunnya tingkat produktivitas yang berakhir pada menurunnya tingkat profit dari yang ditargetkan. Salah satu langkah yang harus dilakukan adalah melakukan perbaikan sistem kerja pada tiap-tiap stasiun kerja. Proses yang dilakukan perbaikan waktu kerja ini adalah dengan membuat peta-peta kerja, yang terdiri dari; Peta Aliran Proses, Peta Pekerja-Mesin, dan Peta Tangan Kiri dan Tangan Kanan; Pengukuran waktu (Time Study) dengan mengambil jenis pengukuran waktu secara langsung dengan menggunakan metode jam henti (Stopwatch) dan sampling pekerjaan; Perancangan dan perbaikan sistem kerja dengan menggunakan studi gerakan, SMED, 7 tool; dan Perhitungan waktu kerja yang dihasilkan dijadikan bahan rujukan pembuatan Standard Operational Procedure (SOP) yang merupakan SOP perbaikan dari SOP sebelumnya.
Kata Kunci : Kondisi Perusahaan, Waktu Kerja, Perbaikan Sistem Kerja
POLA ADAPTASI SOSIAL PEREMPUAN KORBAN PENGGUSURAN DI DKI JAKARTA, MEDAN, DAN MAKASSAR
Authors: Ikhlasiah Dalimoenthe  , Evy Clara  , Yenina Akmal  
Published: July 2021

Penelitian ini bertujuan untuk menganalisis pola adaptasi sosial pada keluarga korban penggusuran di perkotaan, khusunya di DKI Jakarta, Medan, dan Makassar. Dari sisi metodologi, penelitian ini menggunakan paradigma post-positivistik-mixed method. Lokasi penelitian di DKI Jakarta, Medan, dan Makassar dengan waktu penelitian selama 6 bulan, yaitu Mei – Oktober 2020. Subyek penelitian ialah Keluarga korban penggusuran DAS Ciliwung dan Penjaringan (DKI Jakarta); sungai Deli, Medan; dan di jalan A. P. Pettarani 2, jalan Rajawali serta Bara-Baraya, Kota Makassar.Ada empat pola adaptasi sosial yang dilakukan oleh para korban penggusuran yang di relokasi, yaitu adaptasi fisik, sosial, ekonomi, dan teritori. Adaptasi masyarakat pada aspek lingkungan fisik menunjukkan masih dalam tahap penyesuaian diri dari lokasi lama ke lokasi baru. Adaptasi lingkungan sosial tampaknya belum sepenuhnya diterima. Adaptasi Ekonomi yang dilakukan adalah penyesuaian terhadap anggaran rumah tangga, karena pengeluaran menjadi lebih besar. Sementara itu, mengenai adaptasi teritori ada, ada tiga klasifikasi kategori teritori, yaitu: (1) ruang privat); (2) ruang yang bersifat semi-privat; dan (3) ruang publik.
Kata kunci: pola, adaptasi sosial, keluarga korban penggusuran, perkotaan
KOMUNIKASI DALAM KESENIAN SAMPYONG DI MAJALENGKA
Authors: Anggy Giri Prawiyogi  , Jaeni  , Wanda Listiani  
Published: July 2021

Artikel ini bertujuan untuk menggali komunikasi yang terjadi dalam pertunjukan kesenian Sampyong yang berasal dari Kabupaten Majalengka Provinsi Jawa Barat. Metode penelitian yang digunakan yaitu studi literatur dan observasi. Bentuk komunikasi dari kesenian Sampyong ini bisa dilihat dari mulai pertunjukan sampai akhir pertunjukan, dimana terdapat proses komunikasi verbal dan non verbal dalam pelaksanaannya yang tidak hanya melibatkan pemain, tetapi juga penonton dan masyarakat.Komunikasi tersebut tentunya melibatkan banyak pihak, diantaranya pemain, penabuh gamelan, malandang, penonton, dan masyarakat. Hal ini tentu bisa menggambarkan pentingnya komunikasi sebagai salah satu penunjang dalam hidup bermasyarakat dan bekerjasama dengan orang lain dalam hal apapun, tentunya dengan menyadari bahwa kita adalah makhluk sosial yang pasti membutuhkan orang lain.
Kata Kunci : Komunikasi, Kesenian Sampyong.
PERANCANGAN DESAIN SISTEM BASIS DATA PENGELOLAAN ASET (STUDI KASUS: UNIVERSITAS GALUH CIAMIS)
Authors: Dadan Mulyana  , Bacilius Agung Suburdjati  
Published: July 2021

Universitas Galuh adalah perguruan tinggi swasta yang memiliki 7 Fakultas, 1 Program Pascasarjana, Lembaga Penelitian dan Pengabdian Kepada Masyarakat (LPPM), Badan Penjaminan Mutu Internal (BPMI), Perpustakaan dan Kantor Kerjasama. Dari banyaknya tempat yang ada di Universitas Galuh itu menjadi permasalahan yang harus dihadapi dalam manajemen pengelolaan aset. Pengelolaan aset tersebut masih mengandalkan peran dari manusia yang tidak bisa melakukan pendataan aset yang sangatlah banyak. Jika orang tersebut lupa akan menjadi masalah apalagi terkait jenis aset yang melakukan pembayaran dan jika terlambat akan dikenakan sanksi ataupun denda. Penelitian ini untuk memudahkan dalam menghitung jumlah aset dan pencatatan perawatan aset tersebut. Aplikasi pengelolaan aset ini akan bebasis web-based dengan menggunakan bahasa pemerograman php dan MySql sebagai basis datanya. Hasil dari penelitian ini berupa desain basis data yang nantinya akan di implemtasikan pada website yang bisa diakses oleh pengelola aset yang akan melakukan pencatatan perawatan aset dan juga sebagai pengingat akan perawatan aset-aset yang berada di Universitas Galuh.

Kata Kunci: Sistem Informasi, Pengelolaan Aset, ERD, Desain Basis Data
Abstract
Universitas Galuh is a private university that has 7 faculties, 1 Postgraduate Program, Research and Community Service Institute (LPPM), Internal Quality Assurance Agency (BPMI), Library and Cooperation Office. From the many places in Galuh University, it becomes a problem that must be faced in asset management. The management of these assets still relies on the role of human beings who can not do a lot of asset collection. If the person forgets it will be a problem especially related to the type of asset that made the payment and if it is too late will be subject to sanctions or fines. This research is to facilitate in calculating the number of assets and recording the maintenance of these assets. This asset management application will be web-based free by using php and MySql programming languages as its database. The result of this research is a database design that will be implemtasikan on the website that can be accessed by asset managers who will record asset maintenance and also as a reminder of the maintenance of assets located at the University of Galuh.
Keywords :Information System, Asset Management, ERD, Database Design
PENGARUH PARAMETER TEMPERATUR QUENCHING TERHADAP SIFAT MEKANIK DAN STRUKTUR MIKRO REAR HUB SPINDLES
Authors: Murtalim  , Fathan Mubina Dewadi  , Amir  , Wanri Saputra Sigalingging  
Published: July 2021

Perkembangan teknologi terutama dalam pengerasan logam mengalamai kemajuan yang sangat pesat. Perlakuan panas didefenisikan sebagai kombinasi operasi pemanasan dan pendinginan yang terkontrol dalam keadaan padat untuk mendapatkan sifat-sifat tertentu pada baja/logam atau paduan. Salah satu metode perlakuan panas tersebut dengan proses quenching. Proses perlakuan ini pada dasarnya terdiri dari beberapa tahapan, dimulai dengan pemanasan sampai ke temperatur tertentu, lalu diikuti dengan penahanan selama beberapa saat, baru kemudian dilakukan pendinginan dengan kecepatan tertentu. Tujuan penelitian adalah untuk mengetahui perubahan sifat mekanik dan struktur mikro yang terjadi pada material rear hub spindle yang digunakan untuk gigi power thresher setelah megalami proses perlakuan panas dengan media pendinginan oli. Dari hasil pengujian kekuatan bahan memperlihatkan bahwa nilai kekuatan tertingi terdapat pada specimen uji yang diberi perlakuan panas pada temperatur 875 oC dengan nilai kekuatan 602,46 N/mm2 dan nilai kekuatan terkecil pada spesimen uji yang diberi proses perlakuan panas pada temperatur 825 0C dengan nilai kekuatan tarik 537,50 N/mm2. Nilai kekerasan bahan tertinggi terdapat pada spesimen uji yang diberi perlakuan panas pada temperatur 875 oC dengan nilai kekerasan 219 HVN dan nilai kekerasan terkecil terjadi specimen uji yang diberi perlakuan panas pada temperatur 825 oC dengan nilai kekerasan 168 HVN. Dari hasil pengujian mikro struktur memperlihatkan bahwa diameter butiran bahan menunjukkan menurunnya diameter butiran selama proses hardening dengan quenching oli dimana semakin kecil diameter butiran maka sifat mekanis bahan meningkat.
Kata Kunci : Rear Hub Spindles, Perlakuan panas, Sifat Mekanik, Struktur mikro
Analyzing George Bernard Shaw’s Portrayal of Women in the Light of Postfeminist Theory
Authors: Dr. Shahid Abbas  , Dr. Ijaz Asghar  , Qamar Hussain  
Published: July 2021

The paper aims at investigating the critical opinions about Bernard Shaw’s ambivalent relation to feminism. In this regard, the researchers highlight the emerging role of postfeminism and its overlapping elements with the Islamic portrayal of womanhood. Shaw differs from his predecessors drastically – he portrays independent female characters as compared to the invisible and submissive females of the past. Thus, one of the striking features of Shaw’s drama is the depiction of liberated women. The Shavian women do not consider men folk as their rivals. There is a shift from powerless to empowered women in academia. The researchers find out that there is an ideological conflict between feminism and Islam but as far as postfeminism is concerned, there is none. Rather, postfeminism propagates and supports the Islamic concept of womanhood thoroughly. It is also worth noting that feminist ideas and ideology have greatly dented the social and political fabric of mankind and human civilization in general. Whereas, postfeminism propagates in favor of maintaining a balanced position for womanhood in life which is a balance between social and individual life, and a balance between professional and family life. The purpose of this article is to promote a better understanding of the status of women in Islam and its overlapping and common areas with postfeminism, that is, God has equated female folk at par with their male folk. The research is significant as it challenges the western notion of women in Islam and dispels the erroneous notions of suppression of women in Islam. The prime finding of this research is that postfeminism proclaims equal footing for men and women in life, as enshrined in the Holy Quran. Further, the researchers lament that just because of myopic-minded people, the world is not making any progress intellectually. The researchers recommend that there is a dire need to promote liberal intellectuals like Shaw who harbor no bias against Islam and Muslims to maintain peace and order in the world.
The Contribution Of Hand Muscle Power And Wrist Flexibility To Jumping Smash Hit Skill Of Badminton Extracurricular Students At Sman 06 Mukomuko Bengkulu
Authors: Anggara Deffri , Fornandes Bismi 
Published: July 2021

This is a correlational research. The population of this research is all students participating in the badminton extracurricular at SMAN 06 Mukomuko 25 students. The sampling is done by using total sampling method which result in 25 male students. The data collection techniques are : 1) hand power is tested by one hand medicine ball put, 2) wrist flexibility is measured by using arc-ruler, 3) jumping smash hit skill is tested by using badminton court with grading boxes. The data is analysed by product moment correlation and dual correlation with α = 0.05 significance. The result of the data analysis are : 1) There is a significant relation between hand muscle power and students jumping smash hit skill in badminton extracurricular class at SMAN 06 Mukomuko, Bengkulu and the contribution is 39,19%, 2) There is significant relation between wrist flexibility and the students jumping smash hit skill and the contribution is 38,69%, 3) There is significant contribution of both muscle power and wrist flexibility to the students jumping smash hit skill as much as 55,65%.
The Physical Fitness In Volleyball Club at SMAN 4 Of Central Bengkulu
Authors: Saputra Deni Kusuma , Febrianti Mariska , Wibowo Tito Parta 
Published: July 2021

This research was conducted with the aim of knowing the physical fitness in Volleyball Club at SMA N 4 of Central Bengkulu. The design of this research is descriptive quantitative research. The method used in this research is a survey method with data collection techniques using TKJI test. The population of this study were students at SMA N 4 of Central Bengkulu with a total sample of 30 people consisting of 20 male students and 10 female students using total sampling technique. The variables in this study were students at SMA N 4 of Central Bengkulu. The data were collected using the physical fitness test for the 16-19 year category (Depdiknas, 2010: 3), and the data analysis technique used was descriptive statistical techniques with frequency and percentage through categorization. Based on the results of research and data analysis obtained from the physical fitness test in Volleyball Club at SMA N 4 of Central Bengkulu, there were 3 people or 10% were categorized as good, 21 people or 70% were categorized as moderate and 6 people or 20% were categorized as lacking. Based on the results of this study, it can be concluded that the physical fitness of volleyball club at SMA N 4 of Central Bengkulu is in the moderate category.
The Difference Effect Of Explosion Power Of Arm Muscle Exercise And Flexibleness Exercise Observed From Intensity Of Exercise Toward Ability In Front Handspring Gymnastics Athlete In Gymnastics Club Of Semen Padang
Authors: Anggara Deffri , Perdima Feby Elra 
Published: July 2021

This research aims to explain the differences effect of explosion power of arm muscle exercise and flexibleness exercise with 50:50% intensity by explotion power of arm muscle and flexibleness exercising with intensity 70:30% toward front handspring ability of gymnastics athlete in PT Semen Padang Club. The method of this research is quasi experimental method. The population of this research is 31 athletes, but the sample was obtained through purposive random sampling, so it was got 20 female athletes. The data of ability test front handspring aims to get information about ability of front handspring artistics gymnastics athletes of PT Semen Padang Club.Analysis data result shows that: (1) explosion power of arm muscle and flexibleness exercises with 50:50% intensity give significant influence toward ability of front handspring gymnastics athlete in PT Semen Padang Gymnastics Club. The increasing ability of front handspring is 4,1, (2) explosion power of arm muscle exercise and flexibleness exercise with 70:30 % give significant influence toward ability of front handspring gymnastics athlete in PT Semen Padang Gymnastics Club. The increasing ability of front handspring is 7,8, (3) There is difference between explosion power of arm muscle exercise and flexibleness exercise with 50:50% intensity and 70:30% intensity toward ability of front handspring gymnastics athlete in gymnastics club of PT Semen Padang, and explosion power of arm muscle exercise method and flexibleness exercise with 70:30% intensity is better than 50:50% intensity.
Community Health Service Implementation Through Bpjs Health Program
Authors: Hasrillah Hasrilah , Cikusin Yaqub , Hayat Hayat 
Published: July 2021

This research is the result of research that describes the implementation of BPJS Kesehatan in Kedungkandang Health Center, Malang City. The purpose of this research is to know the factors that hinder and support the implementation of the BPJS health program at the Kedungkandang Health Center, and to find out how the BPJS Kesehatan program is implemented at the Kedungkandang Health Center.This study uses several theories, namely Public Policy, Health Service Policy, Implementation. Researchers found 4 factors affecting the implementation process of the BPJS Health program at the Kedungkandang Health Center, namely first, the communication or socialization factor, while the second, the resource factor, from human resources to other resources such as facilities, information, then third, dispotition factors. or the attitude of the bureaucratic apparatus, and fourth, the bureaucratic structure factor. The research method used is qualitative. The research was conducted in the city of Malang, the place is in the Kedungkandang Health Center, the Health Office. Data collection techniques in this study were observation, interviews, and documentation. From research conducted based on field studies in the form of observations and interviews, researchers found that the implementation process at the Kedungkandang Health Center was running quite well, although there were still some obstacles, the Kedungkandang Health Center and the Health Office continued to fix the deficiencies in the process. the implementation of the BPJS program can run well. Based on the research can conclusion The BPJS program is a form of bureaucratic reform in the health sector, because with the BPJS program the community can get treatment more easily and it is not difficult to file documents like the previous health system. With the existence of the BPJS program, it is hoped that it can improve the quality and quality of health service providers to the community in Malang City.
PKM Uji Kompetensi Bidang Keahlian Multimedia Di SMK Sirojul Huda 1 Kota Bogor
Authors: Nurachmad Edi 
Published: July 2021

Uji Kompetensi Keahlian (UKK) merupakan penilaian yang diselenggarakan kepada siswa SMK untuk mengukur pencapaian kompetensi peserta didik yang setara dengan kualifikasi jenjang 2 (dua) atau 3 (tiga) pada KKNI. UKK dilaksanakan di akhir masa studi oleh Lembaga Sertifikasi Profesi atau satuan pendidikan terakreditasi bersama mitra dunia usaha/industri.
Hasil UKK bagi peserta didik akan menjadi indikator ketercapaian standar kompetensi lulusan. Sedangkan bagi stakeholder hasil UKK dijadikan sumber informasi atas kompetensi yang dimiliki calon tenaga kerja. Materi UKK disusun berdasarkan skema sertifikasi sesuai dengan jenjang kualifikasi peserta uji/asesi yang memuat kemampuan melaksanakan pekerjaan spesifik, operasional, dan/atau penjaminan mutu. Soal UKK dapat berbentuk penugasan atau bentuk lain yang dinilai secara individual untuk membuat suatu produk sesuai tuntutan standar kompetensi.
Perangkat UKK yang dikeluarkan oleh Kementerian Pendidikan dan Kebudayaan bersifat terbuka dan peserta uji dapat berlatih menggunakan perangkat ujian tersebut sebelum pelaksanaan ujian.

Kata Kunci : Kompetensi, SMK, Siswa
Peningkatan Kapabilitas UMKM Binaan Rumah Kreatif Bogor Dalam Melakukan Analisa Laporan Keuangan
Authors: Supriadi Yoyon 
Published: July 2021

Increasing the Capability of Micro, Small, and Medium Enterprises (UMKM) Assisted by Bogor Creative Houses in conducting report analysis is intended so that UMKM actors can analyze, evaluate and assess their business performance as well as make useful decisions to be able to make improvements and complete improvements independently of their business activities.This activity is designed systematically, easily and communicatively so that the trainees can easily accept and understand the material presented. In addition, this activity is designed openly so that participants are willing to convey the problems they face so that effective and appropriate ways can be found in accordance with the problems they face.

Keywords: Financial Statement Analysis, Evaluation, Performance and Decisions