bluuebunny/crossref_metadata_2025_split huggingface dataset:
Aluminum depletion induced by co-segregation of carbon and boron in a bcc-iron grain boundary
Authors: Ahmadian A. , Scheiber D. , Zhou X. , Gault B. , Liebscher C. H. , Romaner L. , Dehm G. 
Published: October 2021

AbstractThe local variation of grain boundary atomic structure and chemistry caused by segregation of impurities influences the macroscopic properties of polycrystalline materials. Here, the effect of co-segregation of carbon and boron on the depletion of aluminum at a Σ5 (3 1 0 )[0 0 1] tilt grain boundary in a α − Fe-4 at%Al bicrystal is studied by combining atomic resolution scanning transmission electron microscopy, atom probe tomography and density functional theory calculations. The atomic grain boundary structural units mostly resemble kite-type motifs and the structure appears disrupted by atomic scale defects. Atom probe tomography reveals that carbon and boron impurities are co-segregating to the grain boundary reaching levels of >1.5 at%, whereas aluminum is locally depleted by approx. 2 at.%. First-principles calculations indicate that carbon and boron exhibit the strongest segregation tendency and their repulsive interaction with aluminum promotes its depletion from the grain boundary. It is also predicted that substitutional segregation of boron atoms may contribute to local distortions of the kite-type structural units. These results suggest that the co-segregation and interaction of interstitial impurities with substitutional solutes strongly influences grain boundary composition and with this the properties of the interface.
Morc3 silences endogenous retroviruses by enabling Daxx-mediated histone H3.3 incorporation
Authors: Groh Sophia , Milton Anna Viktoria , Marinelli Lisa Katherina , Sickinger Cara V. , Russo Angela , Bollig Heike , de Almeida Gustavo Pereira , Schmidt Andreas , Forné Ignasi , Imhof Axel , Schotta Gunnar 
Published: October 2021

AbstractEndogenous retroviruses (ERVs) comprise a significant portion of mammalian genomes. Although specific ERV loci feature regulatory roles for host gene expression, most ERV integrations are transcriptionally repressed by Setdb1-mediated H3K9me3 and DNA methylation. However, the protein network which regulates the deposition of these chromatin modifications is still incompletely understood. Here, we perform a genome-wide single guide RNA (sgRNA) screen for genes involved in ERV silencing and identify the GHKL ATPase protein Morc3 as a top-scoring hit. Morc3 knock-out (ko) cells display de-repression, reduced H3K9me3, and increased chromatin accessibility of distinct ERV families. We find that the Morc3 ATPase cycle and Morc3 SUMOylation are important for ERV chromatin regulation. Proteomic analyses reveal that Morc3 mutant proteins fail to interact with the histone H3.3 chaperone Daxx. This interaction depends on Morc3 SUMOylation and Daxx SUMO binding. Notably, in Morc3 ko cells, we observe strongly reduced histone H3.3 on Morc3 binding sites. Thus, our data demonstrate Morc3 as a critical regulator of Daxx-mediated histone H3.3 incorporation to ERV regions.
Direct genome-wide identification of G-quadruplex structures by whole-genome resequencing
Authors: Tu Jing , Duan Mengqin , Liu Wenli , Lu Na , Zhou Yue , Sun Xiao , Lu Zuhong 
Published: October 2021

AbstractWe present a user-friendly and transferable genome-wide DNA G-quadruplex (G4) profiling method that identifies G4 structures from ordinary whole-genome resequencing data by seizing the slight fluctuation of sequencing quality. In the human genome, 736,689 G4 structures were identified, of which 45.9% of all predicted canonical G4-forming sequences were characterized. Over 89% of the detected canonical G4s were also identified by combining polymerase stop assays with next-generation sequencing. Testing using public datasets of 6 species demonstrated that the present method is widely applicable. The detection rates of predicted canonical quadruplexes ranged from 32% to 58%. Because single nucleotide variations (SNVs) influence the formation of G4 structures and have individual differences, the given method is available to identify and characterize G4s genome-wide for specific individuals.
Introducing dorsoventral patterning in adult regenerating lizard tails with gene-edited embryonic neural stem cells
Authors: Lozito Thomas P. , Londono Ricardo , Sun Aaron X. , Hudnall Megan L. 
Published: October 2021

AbstractLizards regenerate amputated tails but fail to recapitulate the dorsoventral patterning achieved during embryonic development. Regenerated lizard tails form ependymal tubes (ETs) that, like embryonic tail neural tubes (NTs), induce cartilage differentiation in surrounding cells via sonic hedgehog (Shh) signaling. However, adult ETs lack characteristically roof plate-associated structures and express Shh throughout their circumferences, resulting in the formation of unpatterned cartilage tubes. Both NTs and ETs contain neural stem cells (NSCs), but only embryonic NSC populations differentiate into roof plate identities when protected from endogenous Hedgehog signaling. NSCs were isolated from parthenogenetic lizard embryos, rendered unresponsive to Hedgehog signaling via CRISPR/Cas9 gene knockout ofsmoothened(Smo), and implanted back into clonally-identical adults to regulate tail regeneration. Here we report thatSmoknockout embryonic NSCs oppose cartilage formation when engrafted to adult ETs, representing an important milestone in the creation of regenerated lizard tails with dorsoventrally patterned skeletal tissues.
Fluorescent glycan fingerprinting of SARS2 spike proteins
Authors: Wu Zhengliang L. , Ertelt James M. 
Published: October 2021

AbstractGlycosylation is the most common post-translational modification and has myriad of biological functions. However, glycan analysis has always been a challenge. Here, we would like to present new techniques for glycan fingerprinting based on enzymatic fluorescent labeling and gel electrophoresis. The method is illustrated on SARS2 spike (S) glycoproteins. SARS2, a novel coronavirus and the causative agent of the COVID-19 pandemic, has had significant social and economic impacts since the end of 2019. To obtain the N-glycan fingerprint of an S protein, glycans released from the protein are first labeled through enzymatic incorporation of fluorophore-conjugated sialic acid or fucose, then separated by SDS-PAGE, and finally visualized with a fluorescent imager. To identify the labeled glycans of a fingerprint, glycan standards and glycan ladders are enzymatically generated and run alongside the samples as references. By comparing the mobility of a labeled glycan to that of a glycan standard, the identity of glycans maybe determined. O-glycans can also be fingerprinted. Due to the lack of an enzyme for broad O-glycan release, O-glycans on the S protein can be labeled with fluorescent sialic acid and digested with trypsin to obtain labeled glycan peptides that are then separated by gel electrophoresis. Glycan fingerprinting could serve as a quick method for globally assessing the glycosylation of a specific glycoprotein.
Concentric ballooned catheterization to the fractional non-newtonian hybrid nano blood flow through a stenosed aneurysmal artery with heat transfer
Authors: Mehmood Obaid Ullah , Bibi Sehrish , Jamil Dzuliana F. , Uddin Salah , Roslan Rozaini , Akhir Mohd Kamalrulzaman Md 
Published: October 2021

AbstractThe current work analyzes the effects of concentric ballooned catheterization and heat transfer on the hybrid nano blood flow through diseased arterial segment having both stenosis and aneurysm along its boundary. A fractional second-grade fluid model is considered which describes the non-Newtonian characteristics of the blood. Governing equations are linearized under mild stenosis and mild aneurysm assumptions. Precise articulations for various important flow characteristics such as heat transfer, hemodynamic velocity, wall shear stress, and resistance impedance are attained. Graphical portrayals for the impact of the significant parameters on the flow attributes have been devised. The streamlines of blood flow have been examined as well. The present finding is useful for drug conveyance system and biomedicines.
Radioablation of Upper Abdominal Malignancies by CT-Guided, Interstitial HDR Brachytherapy: A Multivariate Analysis of Catheter Placement Assisted by Ultrasound Imaging
Authors: Damm Robert , Damm Romy , Heinze Constanze , Surov Alexey , Omari Jazan , Pech Maciej , Powerski Maciej 
Published: October 2021

Purpose To evaluate the use of ultrasound (US) during catheter placement in interstitial brachytherapy (iBT) of abdominal malignancies as an alternative to computed tomography (CT) fluoroscopy.
Materials and Methods Catheter placement for CT-guided iBT was, if US visibility was sufficient, assisted by sonography in 52 consecutive patients with 82 lesions (liver N = 62; kidney N = 16; peritoneum N = 4) of various malignancies. We collected data on lesion visibility, location, depth, size, and dosimetry. Comparison of CT fluoroscopy versus US-assisted catheter placement was performed by Fisher’s exact test for frequencies and U-test for lesion visibility and dosimetric data. Factors predicting the utility of sonography were determined in a lesion-based multivariate regression analysis. A p-value < 0.05 was regarded as statistically significant.
Results 150 catheters (1 to 6 per lesion; mean diameter 3.6 ± 2.4 cm) were implanted. CT fluoroscopy was used for 44 catheters, and US was used for 106 catheters. Lesion visibility assessed by 5-point Likert scale was significantly better in US (median 2 vs. 3; p = 0.011) and effective dose was significantly reduced if US guidance was applicable (median 1.75 vs. 8.19 mSv; p = 0.014). In a multivariate regression analysis, we identified increased lesion size and caudal location within the target organ to independently predict the utility of ultrasound in catheter placement for iBT.
Conclusion Sonography is a helpful technique to assist CT-guided interstitial brachytherapy of upper abdominal malignancies. Especially for larger lesions localized in the lower liver segments or lower half of the kidney, superior visibility can be expected. As the effective dose of the patient is also reduced, radiation exposure of the medical staff may be indirectly lowered.
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Vacuum-Assisted Suction Drainage as a Successful Treatment Option for Postoperative Symptomatic Lymphoceles
Authors: Franke Mareike , Saager Christian , Kröger Jan-Robert , Borggrefe Jan , Mückner Kersten 
Published: October 2021

Purpose Lymphoceles often occur within several weeks or even months after surgery. Mostly asymptomatic and therefore undiagnosed, they may be self-healing without any treatment. A small percentage of postoperative lymphoceles are symptomatic with significant pain, infection, or compression of vital structures, thus requiring intervention. Many different treatment options are described in the literature, like drainage with or without sclerotherapy, embolization of lymph vessels, and surgical approaches with laparoscopy or laparotomy. Inspired by reports stating that postoperative suction drainage can prevent the formation of lymphoceles, we developed a simple protocol for vacuum-assisted drainage of symptomatic lymphoceles, which proved to be successful and which we would therefore like to present.
Materials and Method Between 2008 and 2020, 35 patients with symptomatic postoperative lymphoceles were treated with vacuum-assisted suction drainage (in total 39 lymphoceles). The surgery that caused lymphocele formation had been performed between 8 and 572 days before. All lymphoceles were diagnosed based on biochemical and cytologic findings in aspirated fluid. The clinical and imaging data were collected and retrospectively analyzed.
Results In total, 43 suction drainage catheters were inserted under CT guidance. The technical success rate was 100 %. One patient died of severe preexisting pulmonary embolism, sepsis, and poor conditions (non-procedure-related death). In 94.8 % of symptomatic lymphoceles, healing and total disappearance could be achieved. 4 lymphoceles had a relapse or dislocation of the drainage catheter and needed a second drainage procedure. Two lymphoceles needed further surgery. The complication rate of the procedure was 4.6 % (2/43, minor complications). The median indwelling time of a suction drainage catheter was 8–9 days (range: 1–30 days).
Conclusion The positive effects of negative pressure therapy in local wound therapy have been investigated for a long time. These positive effects also seem to have an impact on suction drainage of symptomatic lymphoceles with a high cure rate.
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NiNP@rGO Nanocomposites as Heterogeneous Catalysts for Thiocarboxylation Cross-Coupling Reactions
Authors: Bandini Marco , Lombardi Lorenzo , Mazzaro Raffaello , Gazzano Massimo , Kovtun Alessandro , Morandi Vittorio , Bertuzzi Giulio 
Published: October 2021

AbstractA new type of ligand-free Ni-nanoparticles supported on rGO (size distribution average d = 9 ± 3 nm) was prepared and fully characterized via morphological (Fe-SEM), structural (P-XRD, HR-TEM), and spectroscopic (ICP-EOS, XPS) analysis tools. The metal composite was effectively employed in the unprecedented heterogeneously Ni-assisted cross-coupling reaction of aryl/vinyl iodides and thiocarboxylates. A range of sulfur-containing aryl as well as vinyl derivatives (15 examples) was achieved in high yields (up to 82%), under mild reaction conditions, and with wide functional group tolerance.
Metástase retroperitoneal, hepática e de linfonodo de osteossarcoma tibial proximal: Relato de caso
Authors: Gutierrez Daniela , Sánchez Carlos A. , Linares Francisco B. , Messa Oscar 
Published: October 2021

ResumoHomem adulto com osteossarcoma na tíbia proximal, tratado com cirurgia de salvamento de membro com endoprótese e quimioterapia. Ele desenvolveu um padrão metastático incomum, comprometendo fígado, ossos e linfonodos inguinais, sem recorrência local na tíbia nem metástases pulmonares. O osteossarcoma (OS) é o segundo tumor ósseo primário mais frequente, depois do mieloma múltiplo em adultos. Os locais frequentes das metástases, em caso de progressão da doença são os pulmões e os ossos. As metástases extrapulmonares são raras. O desenvolvimento de novos esquemas de quimioterapia melhorou a expectativa de vida dos pacientes com osteossarcoma, porém, alterou também os padrões usuais de metástases, resultando em localizações metastáticas incomuns.
Aerosol jet printed 3 omega sensors for thermal conductivity measurement
Authors: Kempf Nicholas , Zhang Yanliang 
Published: October 2021

The 3 omega (3ω) method is a trusted technique for measuring thermal conductivity—a fundamental material property of critical importance in a broad range of applications. However, traditional 3ω sensor processing requires some form of physical vapor deposition, such as metal evaporation or sputtering. These 3ω sensor deposition techniques limit the materials and sample sizes applicable to the 3ω method. This work demonstrates an aerosol jet printing method to directly print silver 3ω sensors that yield accurate temperature-dependent measurement up to 300 °C on materials with thermal conductivity ranging from 1 to 150 W/m K. The interrelationship between printed sensor geometry, sensor thermal stability, and applicability to the 3ω method is examined. Thermal conductivity measurement with 3ω sensors conventionally sintered at 300 °C agrees to independent laser flash measurement within 4% from room temperature to 150 °C. An unconventional rapid high-temperature sintering method is shown to produce sensors that agree within 3% of the laser flash measurements from room temperature to 300 °C. The rapid sintering profiles also reduced the sensor–substrate thermal boundary resistance of the printed sensors by as much as 88%. The direct printing of 3ω sensors creates opportunities for measurement of thermal transport properties in applications previously inapplicable to the 3ω method.
Generalization of the Artin-Hasse logarithm for the Milnor

-groups of

-rings
Authors: Tyurin D. N. 
Published: October 2021

Abstract
Let

be a

-adically complete ring equipped with a

-structure. We construct a functorial group homomorphism from the Milnor

-group

to the quotient of the

-adic completion of the module of differential forms

. This homomorphism is a

-adic analogue of the Bloch map defined for the relative Milnor

-groups of nilpotent extensions of rings of nilpotency degree

for which the number

is invertible.
Bibliography: 12 titles.
Waterlogging as an environmental filter to tree recruitment in tropical wet grasslands
Authors: Ribeiro Jonathan Wesley Ferreira , Reis Gonçalo Rafael , Marta Kolb Rosana 
Published: October 2021

Wet grasslands from the Brazilian Savanna (Cerrado) are open-canopy vegetation dominated by subshrubs and herbaceous plants. Although they frequently occur between gallery forests and savannas, tree species are rarely observed colonising these grasslands. Here, seed germination and seedling development of Cerrado trees under waterlogging conditions were evaluated in order to elucidate how soil waterlogging constrains tree regeneration in tropical wet grasslands. We used seeds of 11 representative Cerrado tree species that have different requirements for soil moisture (non-flooded v. flooded environments). Short periods (15 days) of waterlogging drastically decreased or inhibited germination and seedling development in four species, whereas long periods (30–45 days) of waterlogging reduced or inhibited germination and seedling development in 6 of the 11 species. As expected, we found fewer waterlogging-resistant seeds associated with those species from non-flooded environments. By contrast, more waterlogging-resistant seeds were associated with species that eventually or typically occur in flooded environments. Our results suggest that soil waterlogging is an important environmental filter constraining tree recruitment in tropical wet grasslands. However, some species can overcome this environmental filter by possessing waterlogging-resistant seeds or avoid it by establishing in less waterlogged locations.
Stiffness of targeted layer-by-layer nanoparticles impacts elimination half-life, tumor accumulation, and tumor penetration
Authors: Kong Stephanie M. , Costa Daniel F. , Jagielska Anna , Van Vliet Krystyn J. , Hammond Paula T. 
Published: October 2021

Significance
Layer-by-layer nanoparticles (LbL NPs), comprised of a charged core substrate layered sequentially with oppositely charged polyelectrolytes, are a promising class of drug delivery carriers for cancer therapeutics with demonstrated success in lowering off-target toxicity and enhancing efficacy. However, little is known about how LbL NP stiffness alters trafficking and delivery. Herein, we report that the stiffness of targeted LbL NPs, comprised of a liposome core and tumor-targeting, polymeric outer layers, can be tuned by altering the mechanical properties of its underlying liposomal core. We also show that these changes have a significant impact on in vivo NP trafficking properties; compliant LbL NPs have longer elimination times, higher organ and tumor accumulation, and higher tumor penetration.
1018 Did COVID Compromise the Care of Closed Paediatric Hand Fractures?
Authors: Dbeis R , Yim G , Watts A 
Published: October 2021

Abstract

Aim
To assess the effect of a ‘rapid-access’ musculoskeletal unit, established in March 2020, as part of the Royal Devon & Exeter hospital COVID response, with direct assessment of hand trauma by the Plastic Surgery department staff, on treatment timelines and national guidance compliance for closed paediatric hand fractures.

Method
This was a retrospective review from 1st January to 31st December 2019 and a prospective study from the 1st April to 15th June 2020. The retrospective cases were collated by health informatics. The prospective data was recorded on the department’s database. Exclusion criteria: nail bed injuries with tuft fractures, ligament injuries only, open fractures and patients aged 18 at time of surgery.

Results
In 2019 the majority (73%) of patients (n = 26) were referred within 48hrs but waited a median of 5 days to be seen in clinic by a hand surgeon resulting in significant delays in treatment. After service reconfiguration in 2020, all patients (n = 6) were operated on within 72 hours of the decision to operate - mean time to surgery 1.5 days (range 0-3 days). The mean time from referral to clinic was 1.33 days (range 0-6 days). 4 patients were operated on within 7 days of injury. Of the 2 patients operated on &gt; 7 days, 1 was referred 32 days post injury and 1 failed conservative management.

Conclusions
The introduction of a ‘rapid-access’ musculoskeletal unit with early senior review and a dedicated theatre has created ‘gold-standard’ compliance with national standards for the operative management of closed paediatric fractures.
1050 An Audit of the Management of Acute Epididymo-orchitis
Authors: Sturgess G , Davies A 
Published: October 2021

Abstract

Aim
To evaluate the management of patients referred to our Surgical Assessment Unit presenting with acute epididymo-orchitis. Concordance with local antibiotic guidance, the use of ultrasound scanning, and microbiological testing were all assessed over a one-year period.

Method
All adult patients presenting with acute epididymo-orchitis in 2019 were identified retrospectively using ICD-10 codes and the choice of antibiotic used was compared to our own local guidance. The use of ultrasound scanning was recorded and whether this led to a change in diagnosis or management. Use of microbiological investigations including urine microscopy & culture and sexual health screening were assessed.

Results
After exclusion criteria were applied 64 cases were identified with an average age of 55 (16-88). Concordance with local antibiotic guidance was low with 3/64 patients receiving antibiotics in-line with local guidance. This was predominantly due to the frequent use of Ciprofloxacin (49/64) which is not advised locally, however is in-keeping with guidance from organisations such as EAU/BASHH. Ultrasound was used acutely in 46/64 patients with 28/46 showing evidence of epididymo-orchitis, 15/46 were normal and 1/46 confirmed an abscess which required surgical drainage. All normal scans were still treated with antibiotics based on clinical findings. 25/64 had a urine sample sent for MC&S and uptake of sexual health screening was low.

Conclusions
Urologists should be aware of differences between local prescribing policy and broader guidance from relevant organisations. Epididymo-orchitis remains a clinical diagnosis and ultrasound should be reserved acutely for diagnostic uncertainty, unwell patients, or failure to improve.