bluuebunny/crossref_metadata_2025_split huggingface dataset:
Sex-specific effects of neonatal progestin receptor antagonism on juvenile social play behavior in rats
Authors: Forbes-Lorman R. M. 
Published: November 2021

AbstractDeveloping mammals are exposed to progesterone through several sources; however, the role of progesterone in early development is not well understood. Males express more progestin receptors (PRs) than females within several brain regions during early postnatal life, suggesting that PRs may be important for the organization of the sex differences in the brain and behavior. Indeed, previous studies showed cognitive impairments in male rats treated neonatally with a PR antagonist. In the present study, we examined the role of PRs in organizing juvenile behaviors. Social play behavior and social discrimination were examined in juvenile male and female rats that had been treated with CDB, a PR antagonist, during the first week of postnatal life. Interestingly, neonatal PR antagonism altered different juvenile behaviors in males and females. A transient disruption in PR signaling during development had no effect on social discrimination but increased play initiation and pins in females. These data suggest that PRs play an important role in the organization of sex differences in some social behaviors.
Lnc-RP11-536 K7.3/SOX2/HIF-1α signaling axis regulates oxaliplatin resistance in patient-derived colorectal cancer organoids
Authors: Li Qingguo , Sun Huizhen , Luo Dakui , Gan Lu , Mo Shaobo , Dai Weixing , Liang Lei , Yang Yufei , Xu Midie , Li Jing , Zheng Peiyong , Li Xinxiang , Li Yan , Wang Ziliang 
Published: November 2021

Abstract
Background
Resistance to oxaliplatin is a major obstacle for the management of locally advanced and metastatic colon cancer (CC). Although long noncoding RNAs (lncRNAs) play key roles in CC, the relationships between lncRNAs and resistance to oxaliplatin have been poorly understood yet.

Methods
Chemo-sensitive and chemo-resistant organoids were established from colon cancer tissues of the oxaliplatin-sensitive or -resistant patients. Analysis of the patient cohort indicated that lnc-RP11-536 K7.3 had a potential oncogenic role in CC. Further, a series of functional in vitro and in vivo experiments were conducted to assess the effects of lnc-RP11-536 K7.3 on CC proliferation, glycolysis, and angiogenesis. RNA pull-down assay, luciferase reporter and fluorescent in situ hybridization assays were used to confirm the interactions between lnc-RP11-536 K7.3, SOX2 and their downstream target HIF-1α.

Results
In this study, we identified a novel lncRNA, lnc-RP11-536 K7.3, was associated with resistance to oxaliplatin and predicted a poor survival. Knockout of lnc-RP11-536 K7.3 inhibited the proliferation, glycolysis, and angiogenesis, whereas enhanced chemosensitivity in chemo-resistant organoids and CC cells both in vitro and in vivo. Furthermore, we found that lnc-RP11-536 K7.3 recruited SOX2 to transcriptionally activate USP7 mRNA expression. The accumulative USP7 resulted in deubiquitylation and stabilization of HIF-1α, thereby facilitating resistance to oxaliplatin.

Conclusion
In conclusion, our findings indicated that lnc-RP11-536 K7.3 could promote proliferation, glycolysis, angiogenesis, and chemo-resistance in CC by SOX2/USP7/HIF-1α signaling axis. This revealed a new insight into how lncRNA could regulate chemosensitivity and provide a potential therapeutic target for reversing resistance to oxaliplatin in the management of CC.
Deprescribing to reduce polypharmacy: study protocol for a randomised controlled trial assessing deprescribing of anticholinergic and sedative drugs in a cohort of frail older people living in the community
Authors: Bergler Ulrich , Ailabouni Nagham J. , Pickering John W. , Hilmer Sarah N. , Mangin Dee , Nishtala Prasad S. , Jamieson Hamish ,   Sponsor-investigator
Published: November 2021

Abstract
Background
Targeted deprescribing of anticholinergic and sedative medications in older people may improve their health outcomes. This trial will determine if pharmacist-led reviews lead to general practitioners deprescribing anticholinergic and sedative medications in older people living in the community.

Methods and analysis
The standard protocol items: Recommendations for Interventional Trials (SPIRIT) checklist was used to develop and report the protocol. The trial will involve older adults stratified by frailty (low, medium, and high). This will be a pragmatic two-arm randomized controlled trial to test general practitioner uptake of pharmacist recommendations to deprescribe anticholinergic and sedative medications that are causing adverse side effects in patients.

Study population
Community-dwelling frail adults, 65 years or older, living in the Canterbury region of New Zealand, seeking publicly funded home support services or admission to aged residential care and taking at least one anticholinergic or sedative medication regularly.

Intervention
New Zealand registered pharmacists using peer-reviewed deprescribing guidelines will visit participants at home in the community, review their medications, and recommend anticholinergic and sedative medications that could be deprescribed to the participant’s general practitioner. The total use of anticholinergic and sedative medications will be quantified using the Drug Burden Index (DBI).

Outcomes
The primary outcome will be the change in total DBI between baseline and 6-month follow-up. Secondary outcomes will include entry into aged residential care, prolonged hospitalization, and death.

Data collection points
Data will be collected at the time of interRAI assessments (T0), at the time of the baseline review (T1), at 6 months following the baseline review (T2), and at the end of the study period, or end of study participation for participants admitted into aged residential care, or who died (T3).

Ethics and dissemination
Ethical approval has been obtained from the Human, Disability and Ethics Committee: ethical number (17CEN265).

Trial registration
ClinicalTrials.gov ACTRN12618000729224. Registered on May 2, 2018, with the Australian New Zealand Clinical Trials Registry
The first record of the invasive mosquito species Aedes albopictus in Chişinӑu, Republic of Moldova, 2020
Authors: Șuleșco Tatiana , Bușmachiu Galina , Lange Unchana , Schmidt-Chanasit Jonas , Lühken Renke 
Published: November 2021

Abstract
Background
In Europe, Aedes albopictus is an important vector of chikungunya virus and Dirofilaria nematodes and has been involved in local autochthonous circulation of dengue and Zika viruses. Due to the ongoing spread, targeted field surveillance at potential points of entry of invasive Aedes mosquitoes was initiated by the Republic of Moldova in 2020 as part of the transboundary “Invasive Aedes Mosquitoes COST-Action project.”

Methods
In 2020, ovitraps were positioned at each of three locations: the border crossing to Romania in Leuşeni (Hancesti region), Chişinӑu International Airport and Chişinӑu Botanical Garden.

Results
A total of 188 Aedes spp. eggs were collected at the Chişinӑu International Airport between August and September 2020. Twenty-three adults reared in the laboratory were identified morphologically as Ae. albopictus (Skuse, 1895), and 12 selected specimens were confirmed by molecular barcoding of the cytochrome oxidase subunit I gene region. In addition, one adult Ae. albopictus female at the same site was caught with a manual aspirator.

Conclusions
This is the first documented report of Ae. albopictus in the Republic of Moldova. The presence of immature and adult stages indicates the local reproduction of the species in the country. Therefore, it is crucial to extend and strengthen surveillance of the invasive Aedes mosquitoes to prevent Ae. albopictus and other exotic mosquito species from becoming established in the Republic of Moldova.

Graphical abstract
Germline HLA-B evolutionary divergence influences the efficacy of immune checkpoint blockade therapy in gastrointestinal cancer
Authors: Lu Zhihao , Chen Huan , Jiao Xi , Wang Yujiao , Wu Lijia , Sun Huaibo , Li Shuang , Gong Jifang , Li Jian , Zou Jianling , Yang Keyan , Hu Ying , Mao Beibei , Zhang Lei , Zhang Xiaotian , Peng Zhi , Lu Ming , Wang Zhenghang , Zhang Henghui , Shen Lin 
Published: November 2021

AbstractBackgroundThe human leukocyte antigen class I (HLA-I) genotype has been linked with differential immune responses to infectious disease and cancer. However, the clinical relevance of germline HLA-mediated immunity in gastrointestinal (GI) cancer remains elusive.MethodsThis study retrospectively analyzed the genomic profiling data from 84 metastatic GI cancer patients treated with immune checkpoint blockade (ICB) recruited from Peking University Cancer Hospital (PUCH). A publicly available dataset from the Memorial Sloan Kettering (MSK) Cancer Center (MSK GI cohort) was employed as the validation cohort. For the PUCH cohort, we performed HLA genotyping by whole exome sequencing (WES) analysis on the peripheral blood samples from all patients. Tumor tissues from 76 patients were subjected to WES analysis and immune oncology-related RNA profiling. We studied the associations of two parameters of germline HLA as heterozygosity and evolutionary divergence (HED, a quantifiable measure of HLA-I evolution) with the clinical outcomes of patients in both cohorts.ResultsOur data showed that neither HLA heterozygosity nor HED at the HLA-A/HLA-C locus correlated with the overall survival (OS) in the PUCH cohort. Interestingly, in both the PUCH and MSK GI cohorts, patients with high HLA-B HED showed a better OS compared with low HLA-B HED subgroup. Of note, a combinatorial biomarker of HLA-B HED and tumor mutational burden (TMB) may better stratify potential responders. Furthermore, patients with high HLA-B HED were characterized with a decreased prevalence of multiple driver gene mutations and an immune-inflamed phenotype.ConclusionsOur results unveil how HLA-B evolutionary divergence influences the ICB response in patients with GI cancers, supporting its potential utility as a combinatorial biomarker together with TMB for patient stratification in the future.
Recommendations on service delivery to help reduce suffering and anxiety in patients and caregivers post-hematopoietic cell transplantation: a case report
Authors: Mohammed Jaleel , Bakhsh Hadeel R. , Craig Cliodhna , Hashmi Shahrukh K. 
Published: November 2021

Abstract
Background
The aim of this study is to highlight the importance of having a central case managing team and to make some strong recommendations that can have a positive impact on the lives of hematopoietic stem cell transplantation survivors.

Case presentation
A 2-year-old white child who was diagnosed with acute myeloid leukemia and underwent hematopoietic stem cell transplantation in May 2014 relapsed in March 2017, and underwent a second hematopoietic stem cell transplantation in July 2017, at which point he suffered from graft-versus-host disease. This case report presents his journey and that of his caregivers, and the challenges they faced as patient and parents in pursuit of optimal quality of life during the survivorship period. The case study emphasizes not only the challenges faced by patients but also identified gaps in post-hematopoietic cell transplantation care service delivery. Furthermore, the case study also highlights the importance of involving caregivers in post-transplant care and having a better communication process and service facilitation process throughout the journey of the patient and their carer.

Conclusions
Transplant centers have a duty of care, and a proactive approach with a well-defined pathway is needed for managing post-transplant complications and reducing stress and anxiety for patients and their caregivers.
Enhancing environmental sustainability through corporate governance: the merger and acquisition perspective
Authors: Zheng Dandan , Yuan Zhipan , Ding Shusheng , Cui Tianxiang 
Published: November 2021

Abstract
Background
Environmental sustainability may be perceived as conflicting with economic development. Economic activities such as mergers and acquisitions can contribute to resource allocation optimization, where corporate governance plays an important role in advancing environmental sustainability. Our study broadens the investigation of whether mergers and acquisitions are helpful for sustainable environmental development. We attempt to reveal the mechanism by which mergers and acquisitions affect sustainable development: namely, through corporate governance.

Results
By employing panel data for Chinese listed companies, we reveal that mergers and acquisitions have a significant positive influence on environmental sustainability. In particular, the study documents a mediating effect of corporate governance. We argue that mergers and acquisitions can trigger company reorganizations and thus optimize firm structure, resulting in better corporate governance. This effect, in turn, can enhance company management in several aspects, including sustainable environmental development. Therefore, we conclude that the government can improve corporate governance by promoting board reorganizations via mergers and acquisitions, leading to better environmental sustainability.

Conclusions
This paper can inspire future research avenues by shedding light on how economic development and environment sustainability can be harmonized through better corporate governance. It can also deliver research implications on corporate governance studies through mergers and acquisitions.
Multiparametric prostate MRI quality assessment using a semi-automated PI-QUAL software program
Authors: Giganti Francesco , Lindner Sydney , Piper Jonathan W. , Kasivisvanathan Veeru , Emberton Mark , Moore Caroline M. , Allen Clare 
Published: November 2021

AbstractThe technical requirements for the acquisition of multiparametric magnetic resonance imaging (mpMRI) of the prostate have been clearly outlined in the Prostate Imaging Reporting and Data System (PI-RADS) guidelines, but there is still huge variability in image quality among centres across the world. It has been difficult to quantify what constitutes a good-quality image, and a first attempt to address this matter has been the publication of the Prostate Imaging Quality (PI-QUAL) score and its dedicated scoring sheet. This score includes the assessment of technical parameters that can be obtained from the DICOM files along with a visual evaluation of certain features on prostate MRI (e.g., anatomical structures). We retrospectively analysed the image quality of 10 scans from different vendors and magnets using a semiautomated dedicated PI-QUAL software program and compared the time needed for assessing image quality using two methods (semiautomated assessment versus manual filling of the scoring sheet). This semiautomated software is able to assess the technical parameters automatically, but the visual assessment is still performed by the radiologist. There was a significant reduction in the reporting time of prostate mpMRI quality according to PI-QUAL using the dedicated software program compared to manual filling (5′54″ versus 7′59″; p = 0.005). A semiautomated PI-QUAL software program allows the radiologist to assess the technical details related to the image quality of prostate mpMRI in a quick and reliable manner, allowing clinicians to have more confidence that the quality of mpMRI of the prostate is sufficient to determine patient care.
Project-based learning in science-teacher pedagogical practicum: the role of emotional experiences in building preservice teachers’ competencies
Authors: Tsybulsky Dina , Muchnik-Rozanov Yulia 
Published: November 2021

AbstractThe study investigated preservice teachers’ (PST) emotional experiences, teaching competencies, and the connection between the two over the course of a pedagogical practicum conducted using a project-based learning (PBL) approach. The study addressed the following research questions: (a) Which emotional experiences accompanied PSTs’ PBL-based pedagogical practicum?(b) Of the competencies for implementing PBL that the PSTs developed during the practicum, which did they consider using as part of their classroom practices in the future? (c) Is there a connection between PSTs’ emotional experiences and their self-reported competencies for implementing PBL in their classroom practices? Participants were 16 preservice teachers in their first year in the teacher-education program for teaching sciences. Data were collected from reflective reports, submitted at the end of the first and second semesters, thereby addressing the middle and final stages of the PBL-based practicum, and were analyzed using three complementary methodologies: content, linguistic, and statistical analyses. The findings indicate that, as portrayed by the participants, PSTs’ immersion in the PBL-based practicum was accompanied by both positive and negative emotional experiences. While immersed in the PBL practicum, the PSTs described themselves as developing various teaching competencies for implementing PBL in the classroom. It was also found that the positive emotional experiences outnumbered the negative, and this predominance was positively linked to the development of the PSTs’ competencies.
Targeting cardiomyocyte proliferation as a key approach of promoting heart repair after injury
Authors: Li Shuainan , Ma Wenya , Cai Benzhi 
Published: November 2021

AbstractCardiovascular diseases such as myocardial infarction (MI) is a major contributor to human mortality and morbidity. The mammalian adult heart almost loses its plasticity to appreciably regenerate new cardiomyocytes after injuries, such as MI and heart failure. The neonatal heart exhibits robust proliferative capacity when exposed to varying forms of myocardial damage. The ability of the neonatal heart to repair the injury and prevent pathological left ventricular remodeling leads to preserved or improved cardiac function. Therefore, promoting cardiomyocyte proliferation after injuries to reinitiate the process of cardiomyocyte regeneration, and suppress heart failure and other serious cardiovascular problems have become the primary goal of many researchers. Here, we review recent studies in this field and summarize the factors that act upon the proliferation of cardiomyocytes and cardiac repair after injury and discuss the new possibilities for potential clinical treatment strategies for cardiovascular diseases.
Repetitive accumulation of interstitial cells generates the branched structure of Cladonema medusa tentacles
Authors: Hou Shiting , Zhu Jianrong , Shibata Saki , Nakamoto Ayaki , Kumano Gaku 
Published: November 2021

ABSTRACT
The shaping of tissues and organs in many animals relies on interactions between the epithelial cell layer and its underlying mesoderm-derived tissues. Inductive signals, such as receptor tyrosine kinase (RTK) signaling emanating from mesoderm, act on cells of the epithelium to initiate three-dimensional changes. However, how tissues are shaped in a diploblastic animal with no mesoderm remains largely unknown. In this study, the jellyfish Cladonema pacificum was used to investigate branch formation. The tentacles on its medusa stage undergo branching, which increases the epithelial surface area available for carrying nematocytes, thereby maximizing prey capture. Pharmacological and cellular analyses of the branching process suggest a two-step model for tentacle branch formation, in which mitogen-activated protein kinase kinase signaling accumulates interstitial cells in the future branch-forming region, and fibroblast growth factor signaling regulates branch elongation. This study highlights an essential role for these pluripotent stem cells in the tissue-shaping morphogenesis of a diploblastic animal. In addition, it identifies a mechanism involving RTK signaling and cell proliferative activity at the branch tip for branching morphogenesis that is apparently conserved across the animal kingdom.
Endocytosis mediated by an atypical CUBAM complex modulates slit diaphragm dynamics in nephrocytes
Authors: Atienza-Manuel Alexandra , Castillo-Mancho Vicente , De Renzis Stefano , Culi Joaquim , Ruiz-Gómez Mar 
Published: November 2021

ABSTRACT
The vertebrate endocytic receptor CUBAM, consisting of three cubilin monomers complexed with a single amnionless molecule, plays a major role in protein reabsorption in the renal proximal tubule. Here, we show that Drosophila CUBAM is a tripartite complex composed of Amnionless and two cubilin paralogues, Cubilin and Cubilin2, and that it is required for nephrocyte slit diaphragm (SD) dynamics. Loss of CUBAM-mediated endocytosis induces dramatic morphological changes in nephrocytes and promotes enlarged ingressions of the external membrane and SD mislocalisation. These phenotypes result in part from an imbalance between endocytosis, which is strongly impaired in CUBAM mutants, and exocytosis in these highly active cells. Of note, rescuing receptor-mediated endocytosis by Megalin/LRP2 or Rab5 expression only partially restores SD positioning in CUBAM mutants, suggesting a specific requirement of CUBAM in SD degradation and/or recycling. This finding and the reported expression of CUBAM in podocytes suggest a possible unexpected conserved role for this endocytic receptor in vertebrate SD remodelling.
Local cone beam CT: how did it all start?
Authors: Arai Yoshinori 
Published: November 2021

The mathematical theory of CT was proposed by J. Radon in 1917. It was declared that the projection of whole data sets was needed to reconstruct CT images. Therefore, according to J. Radon’s original theory, local cone beam CT (local CBCT) was impossible to achieve. In this paper, I discuss how local CBCT was discovered and developed. Its development required many technical elements, such as a turntable and X-ray television system, for basic experiments such as those on which narrow collimation theory and multifunctional panoramic tomography were based. These experiments endured many failures during development. Now, local CBCT is extremely popular in dental practice because local CBCT has a low radiation dose and high resolution. This paper introduces the technical elements and outlines the important stages during the development of local CBCT in the 1990s.
Investigation of Reflection from Curved Mirrors on a Plane in the Wolfram Mathematica
Authors: Suncov O. , Zhikharev L. 
Published: November 2021

In this article, the study of the geometry of the flat shapes reflection from curved lines located in the plane of these shapes continues. The paper is devoted to a more detailed description of reflection from the analytical geometry point of view. In addition, the range of proposed tasks has been significantly expanded.
An algorithm for reflecting zero-dimensional and one-dimensional objects from plane curves is compiled, and corresponding illustrations are given.
For the first time, the authors have obtained equations that allow us to construct reflections of a point from second-order curves: a circle, an ellipse, a parabola and a hyperbola, as well as from high-order curves: Bernoulli lemniscates and cardioids [17], [24], [13], [25], [23], [22]. In addition, equations for the reflection results of one-dimensional objects: a segment and a circle, from the same plane curves were obtained. Similar studies are being conducted in the works [2], [1], [32], [28], [3], [4]. All equations are accompanied by blueprints of special cases of reflections obtained using the Wolfram Mathematica mathematical package [18], [19]. In addition, the application contains the source codes, which gives the reader to configure the reflection parameters themselves on condition having access this program, as well as visually assess the change in the reflection pattern when changing these parameters for various types of flat mirrors.
This article demonstrates the possibilities that the obtained equations provide, and the prospects for further work, which consist in obtaining new equations of objects reflected from other flat mirrors.