bluuebunny/crossref_metadata_2025_split huggingface dataset:
Protist diversity and community complexity in the rhizosphere of switchgrass are dynamic as plants develop
Authors: Ceja-Navarro Javier A. , Wang Yuan , Ning Daliang , Arellano Abelardo , Ramanculova Leila , Yuan Mengting Maggie , Byer Alyssa , Craven Kelly D. , Saha Malay C. , Brodie Eoin L. , Pett-Ridge Jennifer , Firestone Mary K. 
Published: April 2021

Abstract
Background
Despite their widespread distribution and ecological importance, protists remain one of the least understood components of the soil and rhizosphere microbiome. Knowledge of the roles that protists play in stimulating organic matter decomposition and shaping microbiome dynamics continues to grow, but there remains a need to understand the extent to which biological and environmental factors mediate protist community assembly and dynamics. We hypothesize that protists communities are filtered by the influence of plants on their rhizosphere biological and physicochemical environment, resulting in patterns of protist diversity and composition that mirror previously observed diversity and successional dynamics in rhizosphere bacterial communities.

Results
We analyzed protist communities associated with the rhizosphere and bulk soil of switchgrass (SG) plants (Panicum virgatum) at different phenological stages, grown in two marginal soils as part of a large-scale field experiment. Our results reveal that the diversity of protists is lower in rhizosphere than bulk soils, and that temporal variations depend on soil properties but are less pronounced in rhizosphere soil. Patterns of significantly prevalent protists groups in the rhizosphere suggest that most protists play varied ecological roles across plant growth stages and that some plant pathogenic protists and protists with omnivorous diets reoccur over time in the rhizosphere. We found that protist co-occurrence network dynamics are more complex in the rhizosphere compared to bulk soil. A phylogenetic bin-based null model analysis showed that protists’ community assembly in our study sites is mainly controlled by homogenous selection and dispersal limitation, with stronger selection in rhizosphere than bulk soil as SG grew and senesced.

Conclusions
We demonstrate that environmental filtering is a dominant determinant of overall protist community properties and that at the rhizosphere level, plant control on the physical and biological environment is a critical driver of protist community composition and dynamics. Since protists are key contributors to plant nutrient availability and bacterial community composition and abundance, mapping and understanding their patterns in rhizosphere soil is foundational to understanding the ecology of the root-microbe-soil system.
Modified endoscopic transnasal orbital apex decompression in dysthyroid optic neuropathy
Authors: Tu Yunhai , Xu Mingna , Kim Andy D. , Wang Michael T. M. , Pan Zhaoqi , Wu Wencan 
Published: April 2021

Abstract
Background
To describe the surgical technique and assess the clinical efficacy and safety of modified endoscopic transnasal orbital apex decompression in the treatment of dysthyroid optic neuropathy.

Methods
In this retrospective research, forty-two subjects (74 orbits) who underwent modified endoscopic transnasal orbital apex decompression for the treatment of dysthyroid optic neuropathy were enrolled. Preoperative and postoperative best-corrected visual acuity (BCVA), visual field mean deviation (MD), Hertel exophthalmometry, and new onset diplopia were assessed before and after the intervention. The Wilcoxon test was used for differential analysis. Linear mixed-models’ analyses were conducted to assess the potential predictors for BCVA change.

Results
Postoperatively, the mean BCVA improved from 0.70 ± 0.62 logMAR to 0.22 ± 0.33 logMAR. BCVA significantly improved in 69 eyes (93%), remained stable in 4 eyes (5%) and deteriorated in 1 eye (1%). MD of visual fields improved from −13.73 ± 9.22 dB to −7.23 ± 7.04 dB. Proptosis decreased from 19.57 ± 3.38 mm to 16.35 ± 3.01 mm. Preoperative BCVA, MD of visual fields and medical rectus diameter were independent factors associated with improvements in BCVA (P < 0.05) by linear mixed-models’ analyses. Eighteen patients (42.9%) developed new diplopia postoperatively.

Conclusion
Modified endoscopic transnasal orbital apex decompression effectively restores vision in dysthyroid optic neuropathy.
YAP, CTGF and Cyr61 are overexpressed in tamoxifen-resistant breast cancer and induce transcriptional repression of ERα
Authors: Kim Hyungjoo , Son Seogho , Ko Yunhyo , Lee Jeong Eon , Kim Sangmin , Shin Incheol 
Published: April 2021

ABSTRACT
About 70% of breast cancers overexpress estrogen receptor α (ERα, encoded by ESR1). Tamoxifen, a competitive inhibitor of estrogen that binds to ER, has been widely used as a treatment for ER-positive breast cancer. However, 20–30% of breast cancer is resistant to tamoxifen treatment. The mechanisms underlying tamoxifen resistance remain elusive. We found that Yes-associated protein (YAP; also known as YAP1), connective tissue growth factor (CTGF; also known as CCN2) and cysteine-rich angiogenic inducer 61 (Cyr61; also known as CCN1) are overexpressed, while ERα is downregulated in tamoxifen-resistant breast cancer. Inhibition of YAP, CTGF and Cyr61 restored ERα expression and increased sensitivity to tamoxifen. Overexpression of YAP, CTGF, and Cyr61 led to downregulation of ERα and conferred resistance to tamoxifen in ER-positive breast cancer cells. Mechanistically, CTGF and Cyr61 downregulated ERα expression at the transcriptional level by directly binding to the regulatory regions of the ERα-encoding gene, leading to increased tamoxifen resistance. Also, CTGF induced Glut3 (also known as SLC2A3) expression, leading to increased glycolysis, which enhanced cell proliferation and migration in tamoxifen-resistant cells. Together, these results demonstrate a novel role of YAP, CTGF and Cyr61 in tamoxifen resistance and provide a molecular basis for their function in tamoxifen-resistant breast cancer.
Ca2+-dependent protein acyltransferase DHHC21 controls activation of CD4+ T cells
Authors: Bieerkehazhi Shayahati , Fan Ying , West Savannah J. , Tewari Ritika , Ko Junsuk , Mills Tingting , Boehning Darren , Akimzhanov Askar M. 
Published: April 2021

ABSTRACT
Despite the recognized significance of reversible protein lipidation (S-acylation) for T cell receptor signal transduction, the enzymatic control of this post-translational modification in T cells remains poorly understood. Here, we demonstrate that DHHC21 (also known as ZDHHC21), a member of the DHHC family of mammalian protein acyltransferases, mediates T cell receptor-induced S-acylation of proximal T cell signaling proteins. Using Zdhhc21dep mice, which express a functionally deficient version of DHHC21, we show that DHHC21 is a Ca2+/calmodulin-dependent enzyme critical for activation of naïve CD4+ T cells in response to T cell receptor stimulation. We find that disruption of the Ca2+/calmodulin-binding domain of DHHC21 does not affect thymic T cell development but prevents differentiation of peripheral CD4+ T cells into Th1, Th2 and Th17 effector T helper lineages. Our findings identify DHHC21 as an essential component of the T cell receptor signaling machinery and define a new role for protein acyltransferases in regulation of T cell-mediated immunity.
The Role of Bak1 Methylation in the Induction of Chromosomal Aberrations under Chronic Low-Intensity External Radiation
Authors: Tsymbal O. , Isubakova D. , Bronikovskaya E. , Milto I. , Khalyuzova M. , Litviakov N. , Takhauova T. 
Published: April 2021

Purpose: To study the relationship between the methylation status of the Bаk1 promoter and the frequency of chromosomal aberrations in human blood lymphocytes under chronic low-intensity external ionizing radiation.
Material and methods: The study was performed on 41 people (31 men and 10 women, aged from 36 to 83 years) who are former or current employees of the Siberian Group of Chemical Enterprises, who have been exposed or haven't been exposed to chronic low-intensity external radiation in the course of their professional activities. The workers included in the study were divided into two groups: the first – 15 people who did not have exposure, the second – 26 people who had external exposure (gamma radiation, total dose 89–716 mSv). Whole blood was used to isolate DNA and evaluate chromosomal aberrations in lymphocytes. The methylation status of the Bаk1 promoter was determined using methylsensitive PCR, which was performed after pretreatment of the isolated DNA with methylsensitive AoxI restrictase. The obtained quantitative data were analyzed using the Statistica 10. The differences were considered statistically significant at p ≤ 0.05.
Results: The methylation status of the Bаk1 promoter in the study groups does not differ (p = 0.18). The study of the effect of external radiation dose on the methylation status of the Bak1 promoter showed that the unmethylated promoter prevails in workers with an average radiation dose of 273.37 ± 43.82 mSv, while the methylated promoter – in workers with an average radiation dose of 183.63 ± 20.58 mSv (p = 0.03). The unmethylated promoter Bаk1 is associated with an increased frequency of chromatid fragments in the blood lymphocytes of group 2 workers (p = 0.03).
Conclusion: The status of methylation of the Bаk1 promoter in human blood lymphocytes under chronic low-intensity ionizing radiation does not change, but it is observed to depend on the radiation dose and is associated with an increased frequency of chromosomal aberrations (chromatid fragments). Thus, the unmethylated Bаk1 promoter prevails when the external radiation dose is increased. An increase in the frequency of chromatid fragments is associated with an unmethylated Bаk1 promoter. In addition, exposure to chronic low-intensity external radiation is accompanied by a decrease in the frequency of chromosomal fragments in the blood lymphocytes of workers of the Siberian Group of Chemical Enterprises.
Evaluation of Ultrahigh Dose Irradiation Sterilization Influence on Cytocompatibility of Decellularized Vascular Scaffolds
Authors: Brumberg V. , Astrelina T. , Kobzeva I. , Nikitina V. , Malivanova T , Usupzhanova D. , Brunchukov V. , Rastorgueva A. , Lischuk S. , Borbat A , Dubova E. , Ganzhelyuk M , Makhova A. , Karaseva T , Dobrovolskaya E , Lomonosova E. , Taratonenkova M , Bushmanov A. , Samoylov A. 
Published: April 2021

Purpose: To study the effect of radiation sterilization at an ultra-high dose of 30 kGy on the cytocompatibility of decellularized vascular scaffolds repopulated with placenta MSCs.
Materials and methods: The material of the study were aortas of laboratory animals (rabbits and rats, three vessels for each animal species), which were subjected to detergent - enzymatic perfusion decellularization by two protocols differing in reagents composition. Then the scaffold decellularized by the most efficient protocol was irradiated at a dose of 30 kGy and repopulated with placenta MSCs. As a control, the unirradiated matrix was seeded with cells of the same type. Histological staining of hematoxilin – eosin, IHC for type I collagen and Ki67, DAPI staining and quantitative assessment of genomic DNA were used to evaluate the effectiveness of decellularization and seeding. Scaffolds seeding was assessed by analyzing serial sections taken on day 1st, 3rd, and 4th of culture.
Results: The scaffolds obtained in accordance with Protocol 1 were characterized by the absence of detectable cell nuclei, while the DNA content in them was significantly lower compared to Protocol 2. On the digitized images of sections of the unirradiated matrix, the cell nuclei were determined for routine H&E and DAPI staining while for the irradiated scaffold the cell nuclei were visualized on the border between the scaffold and fibrin gel only on DAPI stained section at 1st day of culture. The frequency of occurrence of Ki67+ nuclei on the 4th day of culture was significantly lower for the irradiated scaffold in comparison with the non-irradiated scaffold (7.5% and 29.8%, respectively, p=0.0054).
Conclusion: Scaffold irradiation leads to loss of cytocompatibility of tissue-engineering constructs.
Problem of the Relationship of the Developed Oncological Disease with Radiation in Conditions of Production
Authors: Galstyan I. , Nugis V. , Torubarov F. , Kukhta B. , Konchalovsky M. , Kretov A. , Bushmanov A. 
Published: April 2021

Introduction.
Compensation programs for radiation-induced diseases in some foreign countries.
The data presented in UN documents.
The linkage between irradiation and certain diseases in the Russian Federation.
Conclusion.
PENGARUH KRISIS EKONOMI AMERIKA TERHADAP PASAR MODAL INDONESIA DILIHAT DARI ANALISIS LAPORAN KEUANGAN PERUSAHAAN
Authors: Ashshofia Rosetianie Kautsar , Sulistiyani Tina 
Published: April 2021

This study aims to determine whether there are significant differences in the performance of mining companies listed in Indonesia Stock Exchange prior to and during the U.S. economic crisis seen from the analysis of financial statements of companies in 2004-2009. The population in this study were 22 mining companies. Using a purposive sampling technique obtained 10 samples of mining companies. The analysis tools kormogrov-smirnov to test normality of data, paired sample t-test to test normal distribution of data. Wilcoxon test to examine the data distribution is not normal. The ratio shows a significant difference before the economic crisis America during the year 2004-2006 and the American economic crisis of 2007-2009 is the solvency ratio and the ratio of the activity. Ratio showed no significant differences before the economic crisis America during the year 2004-2006 and the U.S. economic crisis in 2007-2009 is the liquidity ratio, profitability ratios and market ratios.
ANALISIS FAKTOR-FAKTOR YANG MEMPENGARUHI KEPUTUSAN KONSUMEN ATAU NASABAH DALAM MEMILIH BANK SEBAGAI TEMPAT MENABUNG DI YOGYAKARTA
Authors: Widhiyanto Galih , Setyono Hendro 
Published: April 2021

This study aims to determine the effect of multipurpose networks ATMs, interest, administrative fees, bank locations and bank services the customer's decision in choosing a bank as a place to save in Yogyakarta. In this study, taking a sample of residents who live in the city Yogyakarta with 60 respondents. This testing using analysis tools in the form of correlation (validity test) and reliability test for know the validity of the data used in research. To test the hypothesis in this research is multiple regression test. In the F test obtained F count (27.594)> F table (2.28) which means that together there is a significant influence between the variable services, bank locations, multipurpose ATM networks, bank interest and administrative fees towards decisions. In the t test there are three variables that have no effect significantly to customer savings decisions, namely service variables with a value of t arithmetic (1.385) <t table (2.0057), Bank Location with a value of t arithmetic (1,933) <t table (2.0057) and Multipurpose And ATM Networks with calculated t values (0.839) <t table (2.0057). While interest and administrative costs are partial each has a significant effect on the savings decision variable customers because the t value obtained for the bank interest (3,697)> t table (2.0057) and administrative costs (3,510) > t table (2.0057). The results of this study provide evidence that 71.9% of customers' savings decisions influenced by the variable service, bank location, multipurpose ATM networks, bank interest and administrative costs while the remaining 28.1% influenced by other factors. Based on data analysis, the factors are a big influence on customer savings decisions is bank interest.
ANALISIS PENGARUH TINGKAT INFLASI, TINGKAT SUKU BUNGA, DAN NILAI TUKAR TERHADAP INDEKS HARGA SAHAM GABUNGAN (IHSG) DI BURSA EFEK INDONESIA (BEI) PERIODE 2013-2015
Authors: Primavistanti Daniar , Sutanto Aftoni 
Published: April 2021

This research aimed to analyze and test the effect of inflation rates, interest rate and exchange rate on the stok price index at the stock exchange in 2013–2015. Independent variable used are inflation, interest rates, and exchange rates. While the dependent variable is the stock price index. The object of this research is in the market listed on the stock price index. The inflation rates, interest rates, and the exchange rate that are taken from Indonesian Bank. The analytical method used is the classic assumption test and regression test. Based on the survey result revealed that in partial inflation and the exchange rate does not significantaly influence the Stock Exchange Composite Index. While the variable interest rate significantly influence the Stock Exchange Composite Index. The test results simultaneosly show variable inflation, interest rates and exchange rates have an influence on the Stock Exchange Composite Index. The coefficient of determination was 28,3%.
PENGARUH HARGA, KUALITAS PRODUK, DAN KUALITAS PELAYANAN TERHADAP KEPUTUSAN PEMBELIAN (STUDI KASUS PADA WARUNG SATE KLATAK PAK PONG BANTUL)
Authors: Adhitama Muhammad Hendri , Sukardi Sukardi 
Published: April 2021

This study aims to analyze the influences of prices, quality of products and services on purchasing decision in Pak Pong Klatak Satay Culinary in Bantul. The subjects of this research were customers of Pak Pong Klatak Satay Culinary in Bantul. The population of the study were people who purchased products of Pak Pong Klatak Satay Culinary in Bantul. The samples were determined by accidental sampling method by total of 80 respondents. Data were collected by giving a list of questions / questionnaires to respondents dealing with prices, products quality as well as services quality and how they influence the customers decision in purchasing the products. This study used a multiple linear regression analysis technique. The researcher tested the hypothesis using T test and F test. The test results showed that prices have a positive but insignificant influence, products quality has a negative and insignificant influence. Meanwhile, services quality has a positive and significant influence. Prices, products and services quality contribute to a simultaneously positive and significant influence upon customer’s purchasing decision as the significant value of 0,000 is smaller than 0,5. The prices variables, products quality and services quality are able to explain the purchasing decision variable in Pak Pong's klatak satay culinary at 35% and the remaining 65% explained by other variables not included in the study.
KUALITAS PRODUK DAN KUALITAS PELANAN SEBAGAI ANTASEDEN KEPUASAN PELANGGAN DALAM MEMBENTUK LOYALITAS PELANGGAN (Studi Pada Pelanggan Waroeng Spesial Sambal “SS” Cabang Veteran Yogyakarta)
Authors: Mulyana Alo Rahmawati , Setyono Hendro 
Published: April 2021

The aim of study is to analyze about effect product quality and service quality on customer’s satisfaction to build customer’s loyalty at Waroeng Spesial Sambal “SS” in the branch of Veteran Yogyakarta. Population in this study are all consumers who make purchases at Waroeng Spesial Sambal “SS” the branch in the branch of Veteran Yogyakarta. While the sample in this study is a portion of consumers who have made purchases at least twice in the past year. The sampling technique uses purposive sampling. The data used in this study is primary data. The number of studies in this study were sixty samples in the city of Yogyakarta which were processed using multiple linear regression analysis and mediation analysis using SPSS 20 for windows. The results of the analysis in this study are: product quality dosen’t have a significant effect on customer satisfaction, service quality has a significant effect on customer satisfaction, customer satisfaction doesn’t have a significant effect on customer loyalty, product quality dosen’t have a significant effect on customer loyalty and service quality has a significant effect on customer loyalty of the two independentvariables dosen’t indicate a mediating relationship with customer satisfaction as a mediator variable.
The worldwide seroprevalence of DENV, CHIKV and ZIKV infection: A systematic review and meta-analysis
Authors: Li Zhihui , Wang Jin , Cheng Xiaomin , Hu Huan , Guo Cheng , Huang Jingyi , Chen Zeliang , Lu Jiahai 
Published: April 2021

Background
As the three major arthropod-borne viruses, dengue virus (DENV), chikungunya virus (CHIKV), and zika virus (ZIKV) are posing a growing threat to global public health and socioeconomic development. Our study aimed to systematically review the global seroprevalences of these arboviruses from existing publications.

Methods
Articles published between Jan 01, 2000 and Dec 31, 2019 in the databases of Embase, Pubmed and Web of Science were searched and collected. Countries or areas with known local presence of Aedes vector mosquitoes were included. Random effects model was utilized to estimate the pooled seroprevalences and the proportion of inapparent infection.

Results
Out of 1375, a total of 133 articles involving 176,001 subjects were included for our analysis. The pooled seroprevalences of DENV, CHIKV and ZIKV were 38%, 25% and 18%, respectively; and their corresponding proportions of inapparent infections were 80%, 40% and 50%. The South-East Asia Region had the highest seroprevalences of DENV and CHIKV, while the Region of the Americas had the highest seroprevalence of ZIKV. The seroprevalences of DENV and CHIKV were similar when comparing developed and developing countries, urban and rural areas, or among different populations. In addition, we observed a decreased global seroprevalences in the new decade (2010–2019) comparing to the decade before (2000–2009) for CHIKV. For ZIKV, the positive rates tested with the nucleic acid detection method were lower than those tested with the antibody detection method. Lastly, numerous cases of dual seropositivity for CHIKV and DENV were reported.

Conclusions
Our results revealed a varied prevalence of arbovirus infections in different geographical regions and countries, and the inapparent infection accounted an unneglected portion of infections that requires more attention. This study will shed lights on our understanding of the true burden of arbovirus infections and promote appropriate vaccination in the future.
Effect of focused ultrasound cavitation augmented with aerobic exercise on abdominal and intrahepatic fat in patients with non-alcoholic fatty liver disease: A randomized controlled trial
Authors: Taha Mona Mohamed , Aneis Yasser M. , Mohamady Heba Mohamed , S. A. Alrasheedy , Elsayed Shereen Hamed 
Published: April 2021

Objectives
The study aimed to examine the effect of focused ultrasound cavitation augmented with aerobic exercise on localized abdominal and intrahepatic fat in fatty liver patients.

Methods
34 fatty liver patients aged 30–45 with a body mass index (BMI) of 30–40 kg/m2 were randomly assigned into two equally numbered groups. Group A received focused ultrasound cavitation and moderate aerobic exercise for three months, while Group B (control group) received moderate aerobic exercise only. Abdominal subcutaneous fat volume, visceral fat volume, liver-to-spleen ratio (L/S ratio), body weight, BMI, and waist circumference were measured both before and after the study period.

Results
Both groups showed significant improvements in subcutaneous fat volume, visceral fat volume, body weight, BMI, and waist circumference relative to baseline where (P < 0.001), with a higher percentage in group A. L/S ratio only showed a significant improvement in group A. Between-group differences were noteworthy regarding L/S ratio and waist circumference where (P < 0.0001).

Conclusion
While substantial risky measures in non-alcoholic fatty liver disease have been modified by aerobic exercise, its combination with focused ultrasound cavitation causes more notable effects on the reduction of abdominal and intrahepatic fat, making it a superior option.

Trial registration
ClinicalTrials.gov: NCT04161703