bluuebunny/crossref_metadata_2025_split huggingface dataset:
Calotropis procera (leaves) supplementation exerts curative effects on promoting functional recovery in a mouse model of peripheral nerve injury
Authors: Zafar Shamaila , Rasul Azhar , Iqbal Javed , Anwar Haseeb , Imran Ali , Jabeen Farhat , Shabbir Asghar , Akram Rabia , Maqbool Javeria , Sajid Faiqa , Arshad Muhammad Umair , Hussain Ghulam , Islam Saiful 
Published: July 2021

AbstractPeripheral nerve injuries are among those complicated medical conditions, which are still waiting for their highly effective first‐line therapies. In this study, the role of Calotropis procera crude leaves was evaluated at different doses for their effectiveness in improving functional recovery following sciatic nerve injury‐induced in the mouse model. Thirty‐two healthy albino mice were divided into four groups as Normal chow group (control, n = 8) and C. procera chow groups (50 mg/kg (n = 8), 100 mg/kg (n = 8) and 200 mg/kg (n = 8)). Behavioral analyses were performed to assess and compare improved functional recovery along with skeletal muscle mass measurement in all groups. Serum samples were analyzed for oxidative stress markers. Results showed that C. procera leaves at dose‐dependent manner showed statistically prominent (p < .05) increase in sensorimotor functions reclamation as confirmed by behavioral analyses along with muscle mass restoration and prominent decline in TOS and momentous increase in TAC along with the augmented activity of antioxidative enzymes.
Nutritional and lifestyle changes required for minimizing the recovery period in home quarantined COVID‐19 patients of Punjab, Pakistan
Authors: Rabail Roshina , Saleem Javeria , Tanveer Zunera , Patching Simon G. , Khalid Abdur Rauf , Sultan Muhammad Tauseef , Manzoor Muhammad Faisal , Karrar Emad , Inam‐Ur‐Raheem Muhammad , Shabbir Muhammad Asim , Aadil Rana Muhammad 
Published: July 2021

AbstractThe COVID‐19 pandemic has introduced a new battle in human history for a safe and fearless life. Therefore, this cross‐sectional survey was conducted (Punjab, Pakistan) on healthy recovered, home quarantined COVID‐19 patients to draw conclusive health support guidelines in the fight against this pandemic. COVID‐19 recovered patients (n = 80) of age ≥14 years were randomly selected during the period November 2020 to February 2021. A nutrition and lifestyle changes questionnaire, containing ten sections and seventy questions, was completed through the telephone/WhatsApp. Data were transferred into an Excel spreadsheet and statistically analyzed by applying chi‐square, correlation, and a t test of independent values using SPSS‐16 software. The patients had an age range of 14 to 80 years, of which 52 (65%) were male and 28 (35%) were female, and 32 (40%) had a normal BMI. The patients had a peak COVID‐19 recovery period of 2 weeks, and a mean recovery period of 2.8 ± 1.4 weeks. Certain variables, including gender (males), age (>40 years), sleep (≤5 hr), less/no physical activity, obesity, diabetes mellitus, and autoimmune diseases, were significantly associated with delayed recovery. Poor nutritional outcomes, including lower intakes of water, legumes, nuts, meat, and milk/yogurt; and higher consumption of fast/fried/junk/spicy foods and cold water/drinks, were also significantly associated with a longer recovery period. The results were similar for not taking daily doses of multivitamins, and vitamins C, D, E, and zinc. This study identified that staying physically active, maintaining sensible body weight, having a sleep of 7 hr, consuming more foods of plant origin especially plant‐based proteins from nuts and legumes, taking supplemental doses of multivitamins, vitamin D, E, and zinc, along with drinking ≥2 L of water daily can provide a significant role in early and safe recovery from COVID‐19.
Glutamine for prevention and alleviation of radiation‐induced oral mucositis in patients with head and neck squamous cell cancer: Systematic review and meta‐analysis of controlled trials
Authors: Alsubaie Hemail M. , Alsini Albaraa Y. , Alsubaie Khaled M. , Abu‐Zaid Ahmed , Alzahrani Faisal R. , Sayed Suhail , Pathak Alok K. , Alqahtani Khaled H. 
Published: July 2021

AbstractWe conducted a systematic review and meta‐analysis of randomized controlled trials (RCTs) that investigated glutamine efficacy in preventing and alleviating radiation‐induced oral mucositis (OM) among patients with head and neck (H&N) cancer. We screened five databases from inception till February 4, 2021 and followed the Preferred Reporting Items for Systematic Reviews and Meta‐Analyses (PRISMA) guidelines. We included 11 RCTs, comprising 922 patients (458 and 464 patients were assigned to glutamine and control group, respectively). The incidence and onset of radiation‐induced OM of any grade did not substantially differ between both groups. However, glutamine substantially reduced the severity of radiation‐induced OM, as reflected by the reduced incidence of severe OM and reduced mean maximal OM grade score. Additionally, glutamine significantly decreased the rates of analgesic opioid use, nasogastric tube feeding, and therapy interruptions. Oral glutamine supplementation demonstrated various therapeutic benefits in preventing and ameliorating radiation‐induced OM among patients with H&N cancer.
Heat transfer performance of wet porous solar collectors under periodic conditions
Authors: Xue Dawei , Liu Yi , Li Ling 
Published: July 2021

AbstractFourier's law was often used to study the heat transfer of collector plates. However, some scholars have found that under time‐varying periodic boundary conditions, using Fourier's law for research will produce certain deviations. In the current work, periodic boundary conditions are used, so the effect of non‐Fourier efficiency on the heat transfer of the collector plate needs to be considered. Based on the Cattaneo–Vernotte equation, a heat transfer model of the solar collector plate with a limited heat transfer rate is constructed, and the problem is solved by the lattice Boltzmann method. On this basis, the influence of radiation, porous media, humidity, and relaxation time on solar collectors is considered. And the average efficiency, tip temperature, and exergy loss of the collectors are quantitatively analyzed. The results show that when the non‐Fourier effect is considered, the tip temperature of the collector plate decreases, the average efficiency and the exergy loss increase. Moreover, with the increase of relaxation time, the tip temperature is lower, the efficiency and the exergy loss are greater. As the humidity and radiation increase, the tip temperature decreases, and the exergy loss and average efficiency increase. When other factors remain unchanged, the average efficiency, tip temperature, and exergy loss increased with an increase in porosity.
Impact of dissipative heat and radiative heat on MHD viscous flow through a slandering stretching sheet with temperature‐dependent variable viscosity
Authors: Sharma Ram Prakash , Ibrahim S. M. , Mishra S. R. , Tinker Seema 
Published: July 2021

AbstractThe purpose of the current investigation is to discuss the varying physical properties, that is, thermal conductivity and viscosity of a viscous time‐independent flow past a slandering stretching surface. The heat transfer phenomenon is enhanced by the inclusion of an external heat source under the action of dissipative heat energy and thermal radiation. The implementation of convective boundary conditions affects the heat and solutal transfer phenomena with the inclusion of chemical reactions. The flow characteristic is directed by the system of differential equations that are converted to nonlinear ordinary type by appropriate assumptions of similarity variables and stream function. Furthermore, the “homotopy analysis method” is deployed to simplify the system. The convergence of the methodology is also discussed with their various order of approximations. The conformity of the solution is presented along with validation in tabular form. Moreover, the effect of the parameters on flow profiles is explained graphically, and the major contribution of few important parameters is laid down. The rate of shear stress along with the longitudinal velocity profile augments with the enhanced variable viscosity. Furthermore, the reverse impact is rendered for the augmented values of the variable viscosity.
The prognostic value of myocardial injury in COVID‐19 patients and associated characteristics
Authors: He Jian , Zhang Bicheng , Zhou Quan , Yang Wenjing , Xu Jing , Liu Tingting , Zhang Haijun , Wu Zhiyong , Li Dong , Zhou Qing , Yan Jie , Zhang Cuizhen , Qian Haiyan , Lu Minjie , Zhou Xiaoyang 
Published: July 2021

AbstractBackgroundSince December 2019, coronavirus disease 2019 (COVID‐19) has emerged as an international pandemic. COVID‐19 patients with myocardial injury might need special attention. However, an understanding on this aspect remains unclear. This study aimed to illustrate clinical characteristics and the prognostic value of myocardial injury to COVID‐19 patients.MethodsThis retrospective, single‐center study finally included 304 hospitalized COVID‐19 cases confirmed by real‐time reverse‐transcriptase polymerase chain reaction from January 11 to March 25, 2020. Myocardial injury was determined by serum high‐sensitivity troponin I (Hs‐TnI). The primary endpoint was COVID‐19‐associated mortality.ResultsOf 304 COVID‐19 patients (median age, 65 years; 52.6% males), 88 patients (27.3%) died (61 patients with myocardial injury, 27 patients without myocardial injury on admission). COVID‐19 patients with myocardial injury had more comorbidities (hypertension, chronic obstructive pulmonary disease, cardiovascular disease, and cerebrovascular disease); lower lymphocyte counts, higher C‐reactive protein (CRP; median, 84.9 vs. 28.5 mg/L; p < .001), procalcitonin levels (median, 0.29 vs. 0.06 ng/ml; p < .001), inflammatory and immune response markers; more frequent need for noninvasive ventilation, invasive mechanical ventilation; and was associated with higher mortality incidence (hazard ratio [HR] = 7.02; 95% confidence interval [CI], 4.45–11.08; p < .001) than those without myocardial injury. Myocardial injury (HR = 4.55; 95% CI, 2.49–8.31; p < .001), senior age, CRP levels, and novel coronavirus pneumonia types on admission were independent predictors to mortality in COVID‐19 patients.ConclusionsCOVID‐19 patients with myocardial injury on admission is associated with more severe clinical presentation and biomarkers. Myocardial injury and higher Hs‐TnI are both strongest independent predictors to COVID‐19‐related mortality after adjusting confounding factors.
Pictorial guide to canine and feline blood smears. Part 1: normal cells
Authors: Papasouliotis Kostas , Murphy Kate 
Published: July 2021

Background: It is important for practitioner's to be able to examine blood smears as part of a patient's haematological assessment. In order to be able to recognise the presence of abnormal cells, it is essential that practitioners can first identify the morphological characteristics of normal blood cells.Aim of the article: This is the first of three articles on practical haematology. The aim of this article is to increase confidence in identifying normal red blood cells, white blood cells and platelets in cats and dogs. The following articles, to be published in subsequent issues, will focus on recognising common haematological abnormalities that can be seen during microscopic examination of blood smears.
Uncertain Kingdom: Nowcasting Gross Domestic Product and its revisions
Authors: Anesti Nikoleta , Galvão Ana Beatriz , Miranda‐Agrippino Silvia 
Published: July 2021

SummaryWe propose a release‐augmented dynamic factor model (RA‐DFM) that allows to quantify the role of a country's data flow in nowcasting both early Gross Domestic Product (GDP) releases and subsequent revisions of official estimates. We use the RA‐DFM to study UK GDP early revision rounds and assemble a comprehensive and novel mixed‐frequency dataset that features 12 years of real‐time data vintages. The RA‐DFM improves over the standard DFM in real time when forecasting the first release each quarter. Economic and survey data help to predict the first revision round.
Knockdown of lncRNA MALAT1 ameliorates acute kidney injury by mediating the miR‐204/APOL1 pathway
Authors: Lu Hai‐Yuan , Wang Guo‐Yi , Zhao Jin‐Wen , Jiang Hai‐Tao 
Published: July 2021

AbstractBackgroundAcute kidney injury (AKI) was characterized by loss of renal function, associated with chronic kidney disease, end‐stage renal disease, and length of hospital stay. Long non‐coding RNAs (lncRNAs) participated in AKI development and progression. Here, we aimed to investigate the roles and mechanisms of lncRNA MALAT1 in AKI.MethodsAKI serum samples were obtained from 129 AKI patients. ROC analysis was conducted to confirm the diagnostic value of MALAT1 in differentiating AKI from healthy volunteers. After hypoxic treatment on HK‐2 cells, the expressions of inflammatory cytokines, MALAT1, miR‐204, APOL1, p65, and p‐p65, were measured by RT‐qPCR and Western blot assays. The targeted relationship between miR‐204 and MALAT1 or miR‐204 and APOL1 was determined by luciferase reporter assay and RNA pull‐down analysis. After transfection, CCK‐8, flow cytometry, and TUNEL staining assays were performed to evaluate the effects of MALAT1 and miR‐204 on AKI progression.ResultsFrom the results, lncRNA MALAT1 was strongly elevated in serum samples from AKI patients, with the high sensitivity and specificity concerning differentiating AKI patients from healthy controls. In vitro, we established the AKI cell model after hypoxic treatment. After experiencing hypoxia, we found significantly increased MALAT1, IL‐1β, IL‐6, and TNF‐α expressions along with decreased miR‐204 level. Moreover, the targeted relationship between MALAT1 and miR‐204 was confirmed. Silencing of MALAT1 could reverse hypoxia‐triggered promotion of HK‐2 cell apoptosis. Meanwhile, the increase of IL‐1β, IL‐6, and TNF‐α after hypoxia treatment could be repressed by MALAT1 knockdown as well. After co‐transfection with MALAT1 silencing and miR‐204 inhibition, we found that miR‐204 could counteract the effects of MALAT1 on HK‐2 cell progression and inflammation after under hypoxic conditions. Finally, NF‐κB signaling was inactivated while APOL1 expression was increased in HK‐2 cells after hypoxia treatment, and lncRNA MALAT1 inhibition reactivated NF‐κB signaling while suppressed APOL1 expression by sponging miR‐204.ConclusionsCollectively, these results illustrated that knockdown of lncRNA MALAT1 could ameliorate AKI progression and inflammation by targeting miR‐204 through APOL1/NF‐κB signaling.
Post‐COVID‐19 Liver Injury: Comprehensive Imaging With Multiparametric Ultrasound
Authors: Radzina Maija , Putrins Davis Simanis , Micena Arta , Vanaga Ieva , Kolesova Oksana , Platkajis Ardis , Viksna Ludmila 
Published: July 2021

ObjectivesThis study aimed to define patterns of liver injury after severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) infection using multiparametric ultrasound (mpUS) in a variable patient population with differing severities of COVID‐19.MethodsNinety patients were enrolled into the study: 56 had SARS‐CoV‐2 3–9 months prior to enrolment; 34 served as a clinically healthy control group. All patients underwent an mpUS evaluation of the liver (elastography, dispersion and attenuation imaging). Seventy‐six patients had abdominal magnetic resonance (MR) and noncontrast enhanced thoracic computed tomography (CT) scans performed at the same day. All patients were screened for biochemical markers of liver injury.ResultsLiver elasticity, viscosity, and steatosis values were significantly altered in patients after COVID‐19, with particularly higher fibrosis scores compared to the control group (P < .001). Increased biochemical markers of liver injury correlated with changes in mpUS (P < .05), but not with findings on CT or MR findings. Seventeen of 34 hospitalized patients had a moderate or severe course of the disease course with more pronounced changes in mpUS. Increased body mass index was found to influence liver injury and correlated with more severe forms of COVID‐19 (P < .001).ConclusionsCOVID‐19 can cause liver injury observable using mpUS. More severe forms of COVID‐19 and patient obesity are related to increased values of liver damage observed. In comparison to MRI and CT, mpUS appears to be more sensitive to involvement of liver parenchyma. Further research is warranted to establish this promising method for evaluating post‐COVID‐19 liver involvement in the aftermath of the pandemic.
Design of 3‐dB parallel‐line coupler using the substrate integrated double‐strip coaxial line with performance enhancement
Authors: Ho Min‐Hua , Lee Keh‐Yi , Hong Wanchu , Su Gwan‐Wei 
Published: July 2021

AbstractThe contribution of this work is to propose a 3‐dB parallel‐line coupler design using the novel substrate integrated double‐strip coaxial line (SIDSCL). The SIDSCL is composed of three substrates with two signal strips embedded in the center of the SIDSCL tunnel. The proposed coupler's coupled‐line section formed by the SIDSCL's two broad‐side‐faced strips resolves the very difficult‐to‐achieved 3‐dB tight coupling task and possesses an extremely wide operating bandwidth. Its flexible circuit layout diversity allows the through‐ and coupled‐port to be laid either on the same side or on the opposite side. A patch capacitance is attached to each branch‐line to compensate for the parasitic inductance and capacitance that are arisen near the branch junction. As a result, the isolation and impedance matching are greatly improved.
Local versus global stress constraint strategies in topology optimization: A comparative study
Authors: da Silva Gustavo Assis , Aage Niels , Beck André Teófilo , Sigmund Ole 
Published: July 2021

AbstractStress‐constrained topology optimization requires techniques for handling thousands to millions of stress constraints. This work presents a comprehensive numerical study comparing local and global stress constraint strategies in topology optimization. Four local and four global solution strategies are presented and investigated. The local strategies are based on either the augmented Lagrangian or the pure exterior penalty method, whereas the global strategies are based on the P‐mean aggregation function. Extensive parametric studies are carried out on the L‐shaped design problem to identify the most promising parameters for each solution strategy. It is found that (1) the local strategies are less sensitive to the continuation procedure employed in standard density‐based topology optimization, allowing achievement of better quality results using less iterations when compared with the global strategies; (2) the global strategies become competitive when P values larger than 100 are employed, but for this to be possible a very slow continuation procedure should be used; (3) the local strategies based on the augmented Lagrangian method provide the best compromise between computational cost and performance, being able to achieve optimized topologies at the level of a pure P‐continuation global strategy with , but using less iterations.
Triple extraction method enables high quality mass spectrometry‐based proteomics and phospho‐proteomics for eventual multi‐omics integration studies
Authors: Sanchez‐Quiles Virginia , Shi Ming‐Jun , Dingli Florent , Krucker Clémentine , Loew Damarys , Bernard‐Pierrot Isabelle , Radvanyi François 
Published: July 2021

AbstractLarge‐scale multi‐omic analysis allows a thorough understanding of different physiological or pathological conditions, particularly cancer. Here, an extraction method simultaneously yielding DNA, RNA and protein (thereby referred to as “triple extraction”, TEx) was tested for its suitability to unbiased, system‐wide proteomic investigation. Largely proven efficient for transcriptomic and genomic studies, we aimed at exploring TEx compatibility with mass spectrometry‐based proteomics and phospho‐proteomics, as compared to a standard urea extraction. TEx is suitable for the shotgun investigation of proteomes, providing similar results as urea‐based protocol both at the qualitative and quantitative levels. TEx is likewise compatible with the exploration of phosphorylation events, actually providing a higher number of correctly localized sites than urea, although the nature of extracted modifications appears somewhat distinct between both techniques. These results highlight that the presented protocol is well suited for the examination of the proteome and modified proteome of this bladder cancer cell model, as efficiently as other more widely used workflows for mass spectrometry‐based analysis. Potentially applicable to other mammalian cell types and tissues, TEx represents an advantageous strategy for multi‐omics on scarce and/or heterogenous samples.
Primary angle closure glaucoma is characterized by altered extracellular matrix homeostasis in the iris
Authors: Semba Richard D. , Zhang Pingbo , Dufresne Craig , Gao Tianshun , Al‐Jadaan Ibrahim , Craven Earl R. , Qian Jiang , Edward Deepak P. , Mahale Alka 
Published: July 2021

AbstractPurposeTo characterize the proteome of the iris in primary angle closure glaucoma (PACG).Experimental DesignIn this cross‐sectional study, iris samples were obtained from surgical iridectomy of 48 adults with PACG and five normal controls. Peptides from iris were analysed using liquid chromatography‐tandem mass spectrometry on an Orbitrap Q Exactive Plus mass spectrometer. Verification of proteins of interest was conducted using selected reaction monitoring on a triple quadrupole mass spectrometer. The main outcome was proteins with a log2 two‐fold difference in expression in iris between PACG and controls.ResultsThere were 3,446 non‐redundant proteins identified in human iris, of which 416 proteins were upregulated and 251 proteins were downregulated in PACG compared with controls. Thirty‐two upregulated proteins were either components of the extracellular matrix (ECM) (fibrillar collagens, EMILIN‐2, fibrinogen, fibronectin, matrilin‐2), matricellular proteins (thrombospondin‐1), proteins involved in cell‐matrix interactions (integrins, laminin, histidine‐rich glycoprotein, paxillin), or protease inhibitors known to modulate ECM turnover (α‐2 macroglobulin, tissue factor pathway inhibitor 2, papilin). Two giant proteins, titin and obscurin, were up‐ and down‐regulated, respectively, in the iris in PACG compared with controls.Conclusions and Clinical RelevanceThis proteomic study shows that ECM composition and homeostasis are altered in the iris in PACG.
Ligand Binding Site Comparison — LiBiSCo — a web‐based tool for analyzing interactions between proteins and ligands to explore amino acid specificity within active sites
Authors: Hassan Sameer , Töpel Mats , Aronsson Henrik 
Published: July 2021

AbstractInteraction between protein and ligands are ubiquitous in a biological cell, and understanding these interactions at the atom level in protein–ligand complexes is crucial for structural bioinformatics and drug discovery. Here, we present a web‐based protein–ligand interaction application named Ligand Binding Site Comparison (LiBiSCo) for comparing the amino acid residues interacting with atoms of a ligand molecule between different protein–ligand complexes available in the Protein Data Bank (PDB) database. The comparison is performed at the ligand atom level irrespectively of having binding site similarity or not between the protein structures of interest. The input used in LiBiSCo is one or several PDB IDs of protein–ligand complex(es) and the tool returns a list of identified interactions at ligand atom level including both bonded and non‐bonded interactions. A sequence profile for the interaction for each ligand atoms is provided as a WebLogo. The LiBiSco is useful in understanding ligand binding specificity and structural promiscuity among families that are structurally unrelated. The LiBiSCo tool can be accessed through https://albiorix.bioenv.gu.se/LiBiSCo/HomePage.py.