bluuebunny/crossref_metadata_2025_split huggingface dataset:
Jasmine tea as a negative oral contrast agent in magnetic resonance cholangiopancreatography (MRCP)
Authors: Utami H S , Mulyantoro D K , Fatimah F 
Published: July 2021

Abstract
Fluids of gastrointestinal system produce a high signal in 2D T2 Half-Fourier Acquisition Single-shot Turbo Spin Echo (HASTE) thick-slab MRCP which can overlap the biliary system image. This is the first experimental study use jasmine tea as oral contrast agent and aimed to evaluate it in improving the image quality of MRCP. This study was tested by phantom and healthy volunteers. Different types of tea phantom were tested which then calculated its signal-to-noise ratio (SNR) value and analyzed the manganese content. 15 healthy volunteers performed 2D T2 HASTE thick-slab MRCP scanning before and after administration of jasmine tea with a variation of 3 minutes, 6 minutes and 9 minutes scanning time. Qualitative analysis was carried out by MRCP questionnaire which was examined by radiologist and quantitative analysis was conducted by measuring the contrast-to-noise ratio (CNR) values. After jasmine tea administration, the image information was significantly different and CNR MRCP was increased compared to before giving negative oral contrast agent. The most optimal time to start scanning was 3 minutes after administration of jasmine tea with a volume of 300 ml (0.648 mg/dl manganese). Jasmine tea can be used as an alternative safe natural negative oral contrast agent and can improve MRCP image quality and reduce the intensity of gastrointestinal signals.
Evaluation of doses distribution in breast cancer mastectomy using SlicerRT
Authors: Jannah F , Hariyanto A P , Aisyah  , Amaliya R , Sarasechan A , Endarko  , Nainggolan A , Rubiyanto A , Nasori  , Haekal M 
Published: July 2021

Abstract
Treatment planning aimed to provide the maximum dose to target tumor and minimize doses received by healthy tissue. Dose-volume histograms contain essential information about the dose distribution that radiation oncologists use in considering a treatment planning. In breast cancer mastectomy, the thickness of the thorax wall has decreased because of surgery. Therefore it is necessary to analyze the dose received by healthy tissue around the tumor. This study analyzes the doses distribution in breast cancer mastectomy using SlicerRT and compares it with treatment planning from the hospital. The study used three data patients from MRCCC Siloam Hospitals Jakarta. The simulation results were analyzed based on the International Commission on Radiation Units and Measurements Report’s 83 for target tumor and tolerance limits of healthy tissue according to limitations from various references. Although the target tumor’s homogeneity does not qualify, the healthy tissue around the tumor is still within tolerance. It is not a problem in intensity-modulated radiation therapy if avoidance of healthy tissue is more important than homogeneity. For the comparison result, the percentage acceptance of SlicerRT simulation is quite good, above 90%.
The role T1-weighted fluid attenuated inversion recovery (FLAIR) post contrast enhancement to improve image quality on MRI brain
Authors: Silfina R O , Indrati R , Utami L R W 
Published: July 2021

Abstract
In general, head MRI examinations are performed using the T1-weighted and T2-weighted sequence techniques. T1-weighted is used to see the anatomy of the brain while T2-weighted aims to see the pathology or abnormalities that occur in the brain. Some of the clinics that are often found in head MRI include tumors, meningitis, epilepsy, intracranial lesions, metastases and others. The use of T1-weighted FLAIR sequences has a long duration of time, but produces a good image and by adding contrast media can show more sensitive abnormalities and minor abnormalities. The aim of the study was to analyze the application of the T1-weighted FLAIR Contrast Enhancement sequence to be more informative than the T1-weighted Contrast Enhancement sequence. This study uses the Systematic Literature Review (SLR) method. There are 80 literatures that have been selected in the initial stage. It was then followed by an extraction process, which produced 10 selected references. There are three stages of extraction, namely looking at the abstract title, identifying inclusion criteria, and identifying exclusion criteria. The results of this study indicate that the T1-weighted FLAIR Contrast Enhancement sequence is more sensitive to minor lesion abnormalities such as clinical parenchymal lesions, meningitis and leptomeningeal inflammatory disease than T1-weighted Contrast Enhancement.
Digestion diatom using Battarbee and Ruhland methods for Pengilon Lake, Dieng, Wonosobo, Central Java, Indonesia
Authors: Rosyada S , Soeprobowati T R 
Published: July 2021

Abstract
Diatoms have been used as a bioindicator of water quality since 1990s. Digestion methods are a fundamental process prior to paleolimnological analysis with the aim to remove organic material from diatom cells which makes it easier for the identification process. This research objective was to evaluate different methods for digestion diatoms that suitable for samples from tropical sediment, especially from Pengilon Lake Dieng, Central Java, Indonesia. Pengilon Lake was formed by eruption of Mount Prahu with clear water and used as an irrigation source. This research was compared Battarbee and Ruhland methods. In the Battarbee method, the samples were freeze-dried, and approximately 1 gram of each sample was measured and used HCl-H2O2 for extraction of the diatom. In the Ruhland method, the samples were freeze-dried, and approximately 1 gram was digested in 10 ml of concentrated HNO3-H2SO4. Under the Battarbee method, at least 21 diatom species were identified, and under the Ruhland method, 23 diatom species were identified. Ruhland method is less time consuming around 33 hours, where the Battarbee method around 73 hours. From the visibility Battarbee method, striae are clear, but some undigested material disturbing identification process. The different results of diatom caused by the imperfect digestion of the organic material, which is influenced by chemical components for extracts organic. As far as the Ruhland method are an appropriate digestion method in a tropical area such as Pengilon Lake.
The effect of coconut sap amount and salt texture on the protein content and total bacterial number in ina sua
Authors: Wattimena S C , Temartenan J S , Lesbatta K J 
Published: July 2021

Abstract

Ina sua is a salted-fermented fish made traditionally by the Teon-Nila-Serua community in the Maluku islands, Indonesia. In addition to salt, ‘sageru’ (coconut sap) is often added in Ina sua production. This study aims to find out the effect of coconut sap amount and salt texture on the protein content and the number of bacteria in the skipjack-Ina sua. This study used a complete randomized design with 3 treatments and 3 replications for each experiment. For coconut sap effect experiment, the amount of coconut sap used were 10 ml/100g fish, 30 ml/100g fish, and 50 ml/100g fish with 30% salt (a mixture of fine and coarse salt). For the salt texture experiment, 30 % coarse salt, 30% fine salt, and a mixture of fine salt (15%) and coarse salt (15%) with 30 ml coconut sap /100g fish were used. The mixtures were incubated at room temperature for two weeks. Analysis of variance and Tuckey test were used to analyse the data. The results show that the amount of coconut sap has a significant effect on the protein content, total bacterial number, and pH in Ina sua, while salt texture has significant effect only on the protein content in Ina sua.
Characterization of green silver nanoparticles of Graptophyllum pictum leaf extract: from the localized surface plasmon resonance to the antimicrobial activity
Authors: Wattimena S C , Silooy D R , Patty P J 
Published: July 2021

Abstract
This study aims to synthesize silver nanoparticles using leaf extract of Graptophyllum pictum and to characterize their properties, starting from localized surface plasmon resonance, functional groups and the particle size distribution, to their antibacterial activity. The measurement of the wavelength of localized surface plasmon resonance was conducted using UV-VIS spectroscopy, while FTIR spectroscopy was used to identify the chemical bonds of organic compounds in the particle. The particle size distribution was analyzed using TEM. The spectrophotometric method was used to assess the antimicrobial properties of the particles against Gram-positive Staphylococcus aureus and Gram-negative Escherichia coli. For this purpose, OD-620 of the bacterial sample was compared to OD-620 of the bacterial sample mixed with the silver nanoparticles, where the data was taken in 24 hours. The wavelength of localized surface plasmon resonance was found to be 455 nm, while FTIR spectrum showed the chemical bonds of organic compounds, denoting the presence of the extract on the particle. The particles were mostly spherical with diameters varying from 5.4 nm to 50.6 nm and the mean diameter was found to be 21.5±9.9 nm. The results from the antimicrobial assessment show that Graptophyllum pictum silver nanoparticles inhibit the growth of both S. aureus and E. coli, where during 24 hours of observation time, the particles affected E. coli, faster than the particles affected S. aureus.
Study of the pineapple peel vinegar potency in restoring the gonadosomatic index of the diabetic rats
Authors: Sitasiwi A J , Jannah S N , Isdadiyanto S , Annisa T , Hermawati C M , Sari A M , Putra M A D 
Published: July 2021

Abstract
The side effect of Diabetes mellitus is the decrease in the reproductive function which could be observed by the Gonadosomatic Index. Gonadosomatic index is a value that expresses the proportion of testis weight to body weight. Pineapple peel vinegar is a diabetic therapy that has been proved in decreasing the blood sugar levels in diabetic rats, but the effect in restoring GSI has not been evaluated yet. This study aimed to evaluate the effect of pineapple peel vinegar in GSI in diabetic rats. Eighteen male rats were divided into 6 groups, i.e., control (C); negative control (Cn), diabetic rats treated with water; positive control (Cp), diabetic rats treated with 0.4 ml apple vinegar; P1, P2, and P3 (diabetic rats s treated with 0,2 ml, 0,4 and 0,8 ml of pineapple peel vinegar). They were treated for 21 days. The day after the last treatment, rats were sacrificed, and testes were isolated and weighted. The GSI was determined by calculated the proportion of the gonad mass to the total body mass. The result showed there was a significant difference (p<0.05) in GSI between treated Cn (0,59 ± 0,05), P1 group (0,62 ± 0,06), P3 (0,70 ± 0,04) when compared to C (0,52 ± 0,03) and Cp (0,36 ± 0,24) groups. The P2 group (0,53 ± 0,08) showed has no significant difference (p>0.05) when compared to C (0,52 ± 0,03) and Cp (0,36 ± 0,24). It could be concluded that pineapple peel vinegar has high potency in restoring the GSI on diabetic rats.
Potential of water hyacinth (Eichhornia crassipes (Mart.) Solms) in Rawapening lake as raw material for fish feed
Authors: Prasetyo S , Anggoro S , Soeprobowati T R 
Published: July 2021

Abstract
Feed is a major factor in determining the success of aquaculture business. The quality and quantity of feeding properly will maximize the growth of fish. Various research and application of technology has been made to obtain alternative feed source so as to reduce the level of dependence on the food production industry. The eutrophication condition of Rawapening Lake has triggered the water hyacinth (Eichhornia crassipes (Mart.) Solms) to grow out of control. It speeds up the loss of water weeds, cover the surface of the water into the fishing area and degrade water quality. The purpose of this study was to determine the potential of water hyacinth as an alternative fish feed ingredient. In addition, it is also an effort to control the growth of water hyacinth in Rawapening Lake. The descriptive analysis method with a quantitative approach was chosen in this study. After making flour, water hyacinth has a protein content of 12.51%. Water hyacinth fine flour mixed with other ingredients including fish meal and fish feed bisamenghasilkan rice bran protein content of 30.2477%. The results of testing the stability of feed in water (Water Stability Feed) showed that the feed was destroyed after being immersed in cold water for 17 hours. The buoyancy test shows the feed is classified as a submerged type. The feed sinks because it has a specific gravity which is greater than the specific gravity of water. The feed hardness test shows that the feed is not destroyed after being given a load of 500 grams. The heavier the load that the feed can hold, it means the feed is getting harder. Protein content of 30.2477% indicates that alternative fish feed has competitive quality because it is able to meet the protein needs of cultivated fish. Especially fish cultivated in tropical areas which have lower protein requirements (20-30%).
Phenetic analysis of turkey berry (Solanum torvum Sw.) based on morphological character in Semarang region
Authors: Martina M , Jumari J , Murningsih M 
Published: July 2021

Abstract
Turkey berry (Solanum torvum Sw.) plant is originated from America, then spread widely to Asian regions. They used as food and pharmaceutical. The differences of latitude affecting morphological variations of Turkey berry since it affecting the physicochemical condition of their habitat, namely temperature and pH. Environmental temperature is one of important factor since it influencing the interaction process, while pH determines the growth and formation of chlorophyll. Morphological variations in turkey berry plant cause a similarity relationship that can examined using phenetic analysis. Currently, studies of turkey berry phenetic analysis based on morphological characters have not been done yet. This study examines the morphological variations of turkey berry that grow in different altitude specifically in the Semarang area. The sampling method which used in this study is the purposive sampling method. Turkey berry samples were obtained from altitude of 300, 600 and 900 above sea level respectively. Data analysis was performed using MVSP (MultiVariate Statistical Package) application. The algorithm used in the MVSP application is UPGMA (Unweighted Pair Group Method with Arithmatic Mean) with the Simple Matching Coefficient method, or grouping method with the complaints of paired pairs by analysis of the distance between pairs. The Operations Taxonomic Units (OTUs) analyzes 27 individuals, with 33 different morphological characters. Phenetic analysis shows the coefficient corelation between OTUs reaches 0.81 which is divided into 2 main clusters and 11 sub-clusters. This shows that turkey berry does not form subspecies to grow in different inner regions.
Application of Doyle and Doyle method for DNA isolation from pamelo yellow orange (Citrus maxima Merr), lime orange (C. limon) and sunkist orange (C. sinensis)
Authors: Mawarni S N , Khairunnisa D , Larasati I , Rizqo N , Erfianti T , Kusumaningrum H P , Ferniah R S , Pujiyanto S 
Published: July 2021

Abstract
Various species of the genus Citrus have grown naturally and were cultivated in Indonesia as a mega-biodiversity country. In recent years, the nutritional content and antioxidant activity of citrus fruits has been used to prevent of the various chronic and degenerative human diseases including potential as an antidote to viruses. Development of the potential and superiority of Indonesian oranges including Pomelo yellow orange (Citrus maxima Merr), lime orange (C. limon) and Sunkist orange (C. sinensis) are needed to be done with molecular characterization that begins with DNA isolation. The study was conducted with the aim of applying the Doyle and Doyle method for DNA isolation to all of the three types of oranges. The method for isolate DNA from plant usually use Cethyl Trimethyl Ammonium Bromide (CTAB) and Sodium Dodecyl Sulphate (SDS). The aim of this study is to isolate DNA from the orange leaves of Pomelo Yellow Orange, Lime Orange and Sunkist Orange with the Doyle & Doyle method. The results of the isolation of orange DNA were measured using NanoDrop. The results showed that the DNA of the three oranges had purity of pomelo yellow oranges 1,83, limes 1.8 and sunkist oranges 1,51. The concentrations obtained from each of the oranges were Yellow Bali Citrus 532 ng / µl, Lime Citrus 664.5 ng / µl and Sunkist Orange 888.6 ng / µl.
Improvement of the potency of microalgae Nannochloropsis and Chaetoceros through antioxidant analysis and optimization of DNA isolation
Authors: Zulkarnain M I , Rahayu H T , Herida A P , Erfianti T , Kusumaningrum H P , Zainuri M , Endrawati H , Widowati I , Haryanti W D U , Mahendrajaya R T 
Published: July 2021

Abstract

Nannochloropsis and Chaetoceros are microalgae that commonly used as natural feed for aquaculture animals. These microalgae are able to produce secondary metabolites that is used as an antioxidant. The objective of the study was to analyze the antioxidant activity from both microalgae using the DPPH method in relation with their DNA quality and quantity. The method for DNA isolation optimation was Doyle and Doyle method with slight modification. The results of this research showed the percentage of inhibition of Nannochloropsis and Chaetoceros were 9.72 % and 5.78%, respectively. While optimization of DNA isolation exhibited that Nannochloropsis was 193,5 ng/ul with a purity of 1,99 while Chaetoceros was 93.9 ng / µl with a purity of 2.00.
Genetic and epigenetic of pain perception
Authors: Nugroho M S , Kamilla D N , Auerkari E I 
Published: July 2021

Abstract
Pain is the common reason why patient seeking for medical help. Recent studies, shows that there are various type of genes that have a role in pain perception. Genetic’s role include pain sensation Genome Wide Association Study, Single Nucleotide Polymorphism, and epigenetic in pain modulation. Recent studies shows that epigenetic mechanism can alter the expression of pronSetyaociceptive or antinociceptive gene that useful in managing pain from now on. In genes there are one or more polymorphisms that effect the expression of the protein products that later affect the pain response. Ion Channel is a protein membrrane that transporting ion in and out of a cell. This ion channel can change depends on the genes that made the protein. Recent studies shown that there’s more than 450 ion channel genes. From all of those ion channels, voltage gated sodium channel (Nav) tend to be investigated more deeply because Nav is the most common and widely distributed in human cell. Changes on those channels can cause neuropathic pain. Small Fibre Neuropathy caused by defect of fiber myelinated A-delta and unmyelinated C. Substitution of a single aminoalkanoic acid in gene SCN9A, a gene that encoding for Na+ channel Nav1.7, can cause changes in channel Nav1.7. Study shows that Small Fibre Neuropathy lesion in gene SCN9A has decreased after surgical pain sensitivity in cohort patients. There’s two types of inherited traits of pain sensation which is Mendellian and Non-Mendellian. Mendellian Inherited Traits show a relation between gene and a specific pain sensation that in recent studies show mutation in Nav1.7 ion channel. However, the Non Mendellian Inherited Traits said that Catechol O Methyltrasnferase, Guanosin Trifosfat, Cyclo Hydrolase and Nav1.9 have a very important role in pain sensation. This gene mutation information can help the clinician to give a better treatment strategies for patients.
Van der woude syndrome
Authors: Elien T , Kamilla D N , Auerkari E I 
Published: July 2021

Abstract
Orofacial clefts (OFCs) are the most common craniofacial birth defect in all populations worldwide and include cleft lip with or without cleft palate (CL/P) and cleft palate alone (CP). Approximately 70% of OFC are non syndromic while 30 % are syndromic. Syndromic OFCs are often caused by structural chromosomal anomalies or by coding mutations in a single gene. The most common OFC syndrome is Van der Woude syndrome (VWS, MIM #119300) which has 2% of all OFCs with an overall prevalence of 1/35,000-1/100,000 live births. The characteristic features of Van der Woude syndrome are orofacial clefts and congenital lower lip pits. IRF6 or GRHL3 plays an important role in VWS mutations. Van der Woude syndrome is inherited in an autosomal dominant pattern, mostly an affected person has one parent with the condition. Patients with Van der Woude Syndrome who has OFCs increased the risk of delayed language development, learning disabilities, or other mild cognitive problems. The average IQ of individuals with van der Woude syndrome is not significantly different. IRF6 or GRHL3 plays an important role in VWS mutations. The IRF6 protein is active in cells that give rise to tissues in the head and face. A shortage of the IRF6 protein affects the development and maturation of tissues in the face, resulting in the signs and symptoms of van der Woude syndrome such as OFCs. GRHL3 were shown to be required for the development of the periderm, and an important player in the IRF6 dependent pathway of periderm development. In this review, author will explain further about Van der Woude Syndrome and review the literature available on recent studies toward the caused of Van der Woude Syndrome whether it is developed from IRF6 genes.
Vitamin D receptor TaqI (rs731236) gene polymorphism in caries patients
Authors: Gani A Z , Zahra P K , Soedarsono N , Yunaini L , Auerkari E I 
Published: July 2021

Abstract
Vitamin D receptor (VDR) is included in the type of protein that serves as the biological function regulator of vitamin D. Tooth formation, especially in enamel and dentin calcification, as well as maintaining the balance of phosphate and calcium ions which is an important factor in protecting teeth requires support from vitamin D. The VDR gene will regulate the activity of VDR proteins. Caries is a multifactorial disease in which genetic factors can affect the host susceptibility to caries. Polymorphism in the VDR gene is suspected to affect the host susceptibility to caries through changes in calcium metabolism. This study aims to discover the VDR gene polymorphism and its association with caries patients in Indonesia. 100 DNA samples from 100 blood samples, including 50 dental caries patients and 50 healthy controls, were analyzed using PCR-RFLP technique. PCR products were digested with the TaqI restrictive enzyme, then assessed with statistical analysis using Fisher’s exact test and Continuity correction test. In the caries group, there were no samples with CC genotype, 4 samples with CT genotype, and 46 samples with TT genotype. There were also 4 C alleles and 96 T alleles. Polymorphic genotypes and alleles were found higher in the caries group (100% and 96%) than healthy controls (88% and 84%). These results conclude that the polymorphism of VDR TaqI (rs731236) gene was found in patients with dental caries. The distribution of genotypes and allele distributions of VDR TaqI (rs731236) gene between caries and healthy controls significantly differs noticeable (p <0.05).