bluuebunny/crossref_metadata_2025_split huggingface dataset:
Narrative Medicine to integrate patients’, caregivers’ and clinicians’ migraine experiences: the DRONE multicentre project
Authors: Tonini Maria Clara , Fiorencis Alessandra , Iannacchero Rosario , Zampolini Mauro , Cappuccio Antonietta , Raddino Raffaella , Grillo Elisabetta , Albanese Maria , Allais Gianni , Bassano Marco André , Brighina Filippo , Carboni Terenzio , Frediani Fabio , Grazzi Licia , Mastrandrea Carmela , Moschiano Franca , Poeta Maria Gabriella , Ranieri Angelo , Turrini Renato , Marini Maria Giulia 
Published: April 2021

Abstract
Background
Although migraine is widespread and disabling, stigmatisation and poor awareness of the condition still represent barriers to effective care; furthermore, research on migraine individual and social impact must be enhanced to unveil neglected issues, such as caregiving burden. The project investigated the migraine illness experience through Narrative Medicine (NM) to understand daily life, needs and personal resources of migraneurs, their caregivers and clinicians, and to provide insights for clinical practice.

Methods
The project involved 13 Italian headache centres and targeted migraneurs, their caregivers and migraine specialists at these centres. Written narratives, composed by a sociodemographic survey and illness plot or parallel chart, were collected through the project’s webpage. Illness plots and parallel charts employed open words to encourage participants’ expression. Narratives were analysed through Nvivo software, interpretive coding and NM classifications.

Results
One hundred and seven narratives were collected from patients and 26 from caregivers, as well as 45 parallel charts from clinicians. The analysis revealed migraine perception in social, domestic and work life within the care pathway evolution and a bond between chaos narratives and day loss due to migraine; furthermore, narratives suggested the extent of the caregiving burden and a risk of underestimation of migraine burden in patients’ and caregivers’ life.

Conclusion
The project represents the first investigation on migraine illness experience through NM simultaneously considering migraneurs’, caregivers’ and clinicians’ perspectives. Comparing narratives and parallel charts allowed to obtain suggestions for clinical practice, while NM emerged as able to foster the pursuing of migraine knowledge and awareness.
Density trends of wild felids in northern Laos
Authors: Rasphone Akchousanh , Kamler Jan F. , Tobler Mathias , Macdonald David W. 
Published: April 2021

AbstractDetermining the density trends of a guild of species can help illuminate their interactions, and the impacts that humans might have on them. We estimated the density trends from 2013 to 2017 of the clouded leopard Neofelis nebulosa, leopard cat Prionailurus bengalensis and marbled cat Pardofelis marmorata in Nam Et—Phou Louey National Park (NEPL), Laos, using camera trap data and spatial capture-recapture models. Mean (± SD) density estimates (individuals/100 km2) for all years were 1.77 ± 0.30 for clouded leopard, 1.50 ± 0.30 for leopard cat, and 3.80 ± 0.70 for marbled cat. There was a declining trend in density across the study years for all three species, with a ≥ 90% probability of decline for clouded leopard and leopard cat and an 83% probability of decline for marbled cat. There was no evidence that mesopredator release occurred as a result of tiger (Panthera tigris) and leopard (P. pardus) extirpations. We believe that snaring, the factor that led to the extirpation of tiger and leopard in NEPL, is now contributing to the decline of smaller felids, to an extent that over-rides any potential effects of mesopredator release on their densities and interactions. We recommend that the NEPL managers implement a more systematic and intensified snare removal program, in concert with extensive community outreach and engagement of local people to prevent the setting of snares. These actions might be the only hope for saving the remaining members of the felid community in NEPL.
Exosomes: a new perspective in EGFR-mutated lung cancer
Authors: Jouida Amina , McCarthy Cormac , Fabre Aurelie , Keane Michael P. 
Published: April 2021

AbstractExosomes are major contributors in cell to cell communication due to their ability to transfer biological material such as protein, RNA, DNA, and miRNA. Additionally, they play a role in tumor initiation, promotion, and progression, and recently, they have emerged as a potential source of information on tumor detection and may be useful as diagnostic, prognostic, and predictive tools. This review focuses on exosomes from lung cancer with a focus on EGFR mutations. Here, we outline the role of exosomes and their functional effect in carcinogenesis, tumor progression, and metastasis. Finally, we discuss the possibility of exosomes as novel biomarkers in early detection, diagnosis, assessment of prognosis, and prediction of therapeutic response in EGFR-mutated lung cancer.
A Comparative Analysis of Parsimonious Yield Curve Models with Focus on the Nelson-Siegel, Svensson and Bliss Versions
Authors: Wahlstrøm Ranik Raaen , Paraschiv Florentina , Schürle Michael 
Published: April 2021

AbstractWe shed light on computational challenges when fitting the Nelson-Siegel, Bliss and Svensson parsimonious yield curve models to observed US Treasury securities with maturities up to 30 years. As model parameters have a specific financial meaning, the stability of their estimated values over time becomes relevant when their dynamic behavior is interpreted in risk-return models. Our study is the first in the literature that compares the stability of estimated model parameters among different parsimonious models and for different approaches for predefining initial parameter values. We find that the Nelson-Siegel parameter estimates are more stable and conserve their intrinsic economical interpretation. Results reveal in addition the patterns of confounding effects in the Svensson model. To obtain the most stable and intuitive parameter estimates over time, we recommend the use of the Nelson-Siegel model by taking initial parameter values derived from the observed yields. The implications of excluding Treasury bills, constraining parameters and reducing clusters across time to maturity are also investigated.
Do teamwork experience and self-regulated learning determine the performance of students in an online educational technology course?
Authors: Oyelere Solomon Sunday , Olaleye Sunday Adewale , Balogun Oluwafemi Samson , Tomczyk Łukasz 
Published: April 2021

AbstractThis study uses the quantitative research approach to examine the connection between students’ teamwork experience, self-regulated learning, technology self-efficacy, and performance in an online educational technology course. Sixty-three (63) students participated in this study. The study data were collected through an online questionnaire that included background information, course satisfaction, motivation strategies for learning, and online technology self-efficacy, to study the variables’ interactions using quantitative research. To realize this study’s aims, multivariate regression and correlation approaches were employed to analyze the online students’ data. The multivariate regression analysis results show a relationship between self-regulated learning, the online course level, and the number of online courses that the students have completed. Right self-regulated learning strategies in online courses motivate students to strive for a good teamwork experience, leading to increased interest in online learning. In addition, the results also show that there is a relationship between satisfaction and the level of the online course. Achieving good grades makes the student more satisfied and improves the level of technology use. Finally, this study established a relationship between the students’ motivation and the online course level. Therefore, teachers and course designers should implement learning objects that promote students’ engagement and motivation in online learning environments.
High-power, short-duration ablation in the coronary sinus: clinical cases and preliminary observations on swine hearts
Authors: Ma Chengming , Yin Xiaomeng , Xia Yunlong , Sun Jiao , Dai Shiyu , Gao Lianjun , Xiao Xianjie , Sun Yuanjun , Zhang Rongfeng , Dong Yingxue , Wang Zhongzhen , Yu Xiaohong 
Published: April 2021

Abstract
Purpose
Coronary sinus-related arrhythmias are common; however, it is difficult to perform radiofrequency (RF) ablation at these sites efficiently and safely. High-power, short-duration ablation (HPSD) is a proven alternative strategy for pulmonary vein isolation (PVI); whether it can be applied to ablation of the coronary sinus is unknown. The purpose of this preliminary study was to evaluate the feasibility and safety of HPSD ablation in the coronary sinus.

Methods
Firstly, we demonstrated 4 clinical cases of 3 types of arrhythmias who had unsuccessful ablation with standard power initially, but received successful ablations with HPSD. Secondly, RF ablation was performed in the coronary sinus ostium (CSO) and middle cardiac vein (MCV) of 4 in vitro swine hearts. Two protocols were compared: HPSD (45 W/5 S×5 rounds) and a conventional strategy that used low-power, long-duration ablation (LPLD: 25 W/10 S ×5 rounds). The total duration of HPSD protocol was 25 s, and which of LPLD was 50 s.

Results
A total of 28 lesions were created. HPSD can produce longer, wider, deeper, and larger lesions than LPLD. This difference was more pronounced when the ablation was in the MCV. One instance of steam pop occurred during LPLD in the MCV.

Conclusions
HPSD is an effective alternative strategy for ablation in coronary sinus according to clinical applications and preliminary animal study. However, the safety needs to be further evaluated based on more animal and clinical studies.
Intraoperative impaired cerebrovascular autoregulation and delayed neurocognitive recovery after major oncologic surgery: a secondary analysis of pooled data
Authors: Kahl Ursula , Rademacher Cornelius , Harler Ulrich , Juilfs Neelke , Pinnschmidt Hans O. , Beck Stefanie , Dohrmann Thorsten , Zöllner Christian , Fischer Marlene 
Published: April 2021

AbstractCerebral blood flow is tightly regulated by cerebrovascular autoregulation (CVA), and intraoperative impairment of CVA has been linked with perioperative neurocognitive disorders. We aim to assess whether impairment of CVA during major oncologic surgery is associated with delayed neurocognitive recovery (DNCR) postoperatively. We performed a secondary analysis of prospectively collected data. Patients were included if they had undergone complete pre- and postoperative neuropsychological assessments, continuous intraoperative measurement of CVA, and major oncologic surgery for visceral, urological, or gynecological cancer. Intraoperative CVA was measured using the time-correlation method based on near-infrared-spectroscopy, and DNCR was assessed with a neuropsychological test battery. A decline in cognitive function before hospital discharge compared with a preoperative baseline assessment was defined as DNCR. One hundred ninety-five patients were included in the analysis. The median age of the study population was 65 years (IQR: 60–68); 11 patients (5.6%) were female. Forty-one patients (21.0%) fulfilled the criteria for DNCR in the early postoperative period. We found a significant association between impaired intraoperative CVA and DNCR before hospital discharge (OR = 1.042 [95% CI: 1.005; 1.080], p = 0.028). The type of surgery (radical prostatectomy vs. other major oncologic surgery; OR = 0.269 [95% CI: 0.099; 0.728], p = 0.010) and premedication with midazolam (OR = 3.360 [95% CI: 1.039; 10.870], p = 0.043) were significantly associated with the occurrence of DNCR in the early postoperative period. Intraoperative impairment of CVA is associated with postoperative neurocognitive function early after oncologic surgery. Therefore, intraoperative monitoring of CVA may be a target for neuroprotective interventions. The initial studies were retrospectively registered with primary clinical trial registries recognized by the World Health Organization (ClinicalTrials.gov Identifiers: DRKS00010014, 21.03.2016 and NCT04101006, 24.07.2019).
Matrix composition mediates effects of habitat fragmentation: a modelling study
Authors: Chetcuti Jordan , Kunin William E. , Bullock James M. 
Published: April 2021

Abstract
Context
Habitat loss has clear negative effects on biodiversity, but whether fragmentation per se (FPS), excluding habitat loss does is debatable. A contribution to this debate may be that many fragmentation studies tend to use landscapes of fragmented focal-habitat and a single vastly different species-poor intervening land cover (the matrix).

Objectives
How does matrix composition influence the effect of FPS on biodiversity?.

Methods
Using an individual-based model to investigate the effect of different configurations of the matrix on the relationship between FPS and biodiversity of the focal-habitat. We manipulated the number and quality of land cover types in the matrix, and their similarity to the focal-habitat.

Results
Extremely different matrix, caused an order of magnitude stronger effect of FPS on alpha- and gamma-diversity and beta-diversity to decline. Low FPS led to high gamma-diversity. Increasing FPS caused a dramatic decline to low diversity. In contrast landscapes with a more similar matrix had lower diversity under low FPS declining little with increasing FPS. Having few matrix types caused beta-diversity to decline in general compared to landscapes with a larger numbers.

Conclusions
The effects of FPS on biodiversity may change depending on the number of matrix types and their similarity to the focal-habitat. We recommend that fragmentation studies should consider a greater variety of landscapes to help assess in which cases FPS does not have a negative impact and allow better predictions of the impacts of fragmentation. We show the importance of having a diversity of matrix land cover types and improving the hospitability of the matrix for species dependent on the focal-habitat.
GliPR1 knockdown by RNA interference exerts anti‐glioma effects in vitro and in vivo
Authors: Scheuring Urban J. , Ritter Steffi , Martin Daniel , Schackert Gabriele , Temme Achim , Tietze Stefanie 
Published: April 2021

Abstract
Introduction
In human glioblastomas, glioma pathogenesis-related protein1 (GliPR1) is overexpressed and appears to be an oncoprotein. We investigated whether GliPR1 knockdown in glioma cells by RNA interference exerts anti-glioma effects.

Methods
Experiments used human glioblastoma cell lines transduced with GliPR1 shRNA (sh#301, sh#258). Transduction produced stringent doxycycline-dependent GliPR1 knockdown in clones (via lentiviral “all-in-one” TetOn-shRNA vector) or stable GliPR1 knockdown in polyclonal cells (via constitutive retroviral-shRNA vector). In vitro assessments included cellular proliferation and clonogenic survival. In vivo assessments in tumor-bearing nude mice included tumor growth and survival.

Results
Using doxycycline-dependent GliPR1 knockdown, shGliPR1-transduced U87-MG clones demonstrated reductions in cellular proliferation in the presence versus absence of doxycycline. Using stable GliPR1 knockdown, polyclonal shGliPR1-transduced U87-MG, A172, and U343-MG cells consistently showed decreased clonogenic survival and induced apoptosis (higher proportion of early apoptotic cells) compared to control shLuc-transduced cells. In tumor-bearing nude mice, using doxycycline-dependent GliPR1 knockdown, subcutaneous and cranial transplantation of the U87-MG clone 980-5 (transduced with GliPR1 sh#301) resulted in reduced subcutaneous tumor volume and cerebral tumor area in doxycycline-treated mice versus those left untreated. Using stable GliPR1 knockdown, nude mice cranially transplanted with polyclonal U87-MG cells transduced with GliPR1 sh#258 had significantly prolonged survival compared to mice cranially transplanted with control shLuc-transduced cells (41 versus 26 days; P < 0.001).

Conclusion
GliPR1 knockdown in glioma cells decreased cellular proliferation, decreased clonogenic survival, and induced apoptosis in vitro, and reduced glioblastoma tumor growth and prolonged survival in vivo. These findings support that GliPR1 may have potential value as a therapeutic target.
The unintended benefit of the risk factor mandate of 2005
Authors: Huang Allen H. , Shen Jianghua , Zang Amy Y. 
Published: April 2021

AbstractIn 2005, the SEC mandated that firms disclose risk factors to provide useful information about firm risk. An unintended effect of the mandate is that mandatory risk factor (RF) disclosure may constitute “meaningful cautionary language” as defined in the Private Securities Litigation Reform Act, and may therefore provide legal protection for forward-looking statements (FLSs). Using both a difference-in-differences design and a two-stage least squares approach, we find that, following the mandate, firms that had not previously disclosed risk factors (late RF disclosers) became more willing to provide qualitative FLSs, particularly positive ones, than other firms. This finding is consistent with our prediction that, for late RF disclosers, the mandate reduces managers’ perceived litigation risk. We also find that these firms experience improvement in their information environment. A path analysis reveals that the mandate improves firms’ information environment not only directly but also indirectly by prompting more disclosure of positive FLSs, illustrating an unintended benefit of the 2005 RF mandate. Cross-sectional tests show that the RF mandate induces a larger increase in positive FLSs for firms whose managers perceive a higher level of benefit from safe harbor protection arising from meaningful cautionary statements.
Re-examining female labor supply responses to the 1994 Australian pension reform
Authors: Morris Todd 
Published: April 2021

AbstractMany governments are aiming to extend working lives by raising the age at which people can claim retirement pensions. This makes it vital to understand how these policies affect retirement decisions. In this paper, I revisit the labor supply effects of a major Australian reform that increased women’s pension age from 60 to 65. Atalay and Barrett (2015) studied these effects using repeated household surveys and a differences-in-differences design in which male cohorts form the comparison group. They estimate that the reform increased female labor force participation by 12 percentage points. Using earlier data, I show that the parallel-trends assumption did not hold before the reform because of a strong female-specific trend in participation rates across the relevant cohorts. Accounting for this trend, the estimated effect on female participation falls by two-thirds and becomes statistically insignificant at conventional levels. This highlights the importance of carefully assessing and controlling for trends across cohorts when evaluating pension reforms, which are typically phased in across cohorts.
Mitochondria-DNA copy-number and incident venous thromboembolism among middle-aged women: a population-based cohort study
Authors: Nymberg Peter , Memon Ashfaque A. , Sundquist Jan , Sundquist Kristina , Zöller Bengt 
Published: April 2021

AbstractVenous thromboembolism (VTE) is the third most common cardiovascular disease. Low amount of mitochondrial DNA copy number (mtDNA-CN) has been associated with arterial cardiovascular disease (CVD) and reflects mitochondrial dysfunctions. However, whether mtDNA-CN is associated with VTE has not been determined. To examine the association between mtDNA-CN and incident VTE among middle-aged women. 6917 women aged 50–64 years, followed for 20 years in the Women’s Health In the Lund Area (WHILA) study. DNA samples for mtDNA quantification were available from 2521 women. Quantification of mtDNA-CN was performed using a well-optimized droplet digital PCR method. After exclusions of women with anticoagulant treatment, women living in nursing homes, and women who were diagnosed with cancer, stroke, VTE, or coronary heart disease at baseline, a cohort of 2117 women remained for analysis. Cox regression was used to analyze the relationship between mtDNA-CN and time to VTE (hazard ratio = HR). In total, 87 women were diagnosed with VTE during follow-up, corresponding to an incidence rate of 2.8 per 1000 person-years. Neither crude nor adjusted HR for mtDNA-CN were significantly associated with incident VTE. A sensitivity analysis with inclusion of excluded women did not change the results. MtDNA-CN was not significantly associated with VTE. The present study suggests that mtDNA-CN, reflecting mitochondrial dysfunction, should not be considered a biomarker that plays a major role for developing VTE. However, due to limited study size we may not exclude minor associations.
Detection of mink astrovirus in Poland and further phylogenetic comparison with other European and Canadian astroviruses
Authors: Jakubczak Andrzej , Kowalczyk Marek , Mazurkiewicz Ilona , Kondracki Marcin 
Published: April 2021

AbstractMink astrovirus infection remains a poorly understood disease entity, and the aetiological agent itself causes disease with a heterogeneous course, including gastrointestinal and neurological symptoms. This paper presents cases of astrovirus infection in mink from continental Europe. RNA was isolated from the brains and intestines of animals showing symptoms typical of shaking mink syndrome (n = 6). RT-PCR was used to amplify astrovirus genetic material, and the reaction products were separated on a 1% agarose gel. The specificity of the reaction was confirmed by sequencing fragment coding RdRP protein (length of sequencing product 170 bp) from all samples. The presence of astrovirus RNA was detected in each of the samples tested. Sequencing and bioinformatic analysis indicated the presence of the same variant of the virus in all samples. Comparison of the variant with the sequences available in bioinformatics databases confirmed that the Polish isolates form a separate clade, closely related to Danish isolates. The dissimilarity of the Polish variant to those isolated in other countries ranged from 2.4% (in relation to Danish isolates) to 7.1% (in relation to Canadian isolates). Phylogenetic relationships between variants appear to be associated with the geographic distances between them. To our knowledge, this work describes the first results on the molecular epidemiology of MAstV in continental Europe. The detection of MAstV in Central Europe indicates the need for further research to broaden our understanding of the molecular epidemiology of MAstV in Europe.