bluuebunny/crossref_metadata_2025_split huggingface dataset:
First Report of SYNE1 Arthrogryposis Multiplex Congenita From Saudi Arabia With a Novel Mutation: A Case Report
Authors: Kamal Naglaa , Alzeky Alaaeddin , Omair Maher R , Attar Ruwayd , Alotaibi Abdullah , safar Abdullah 
Published: November 2021

Abstract
Introduction: Arthrogryposis multiplex congenita (AMC), is a rare congenital condition characterized by multiple joint contractures often affecting both arms and legs which start prior to birth.Patient concern: The pediatrician attending delivery noticed the baby being dysmorphic with generalized hypotonia and multiple joint contractures at birth with antenatal history of reduced fetal movement. The patient was admitted to the neonatal intensive care unit for strict observation and further work up.Diagnosis: Whole Exome Sequencing was performed and identified the novel homozygous variant in the synaptic nuclear envelope protein1 [SYNE1] gene.Intervention: Multidisciplinary team were involved in the management plan, supportive care was the mainstay of treatment.Outcomes: Due to feeding difficulties and on and off requirement of oxygen support, the patient remained hospitalized for a long period then was discharged home on supplemental oxygen and gastrostomy tube feeding.Conclusions: AMC3 should be suspected in patients with decreased fetal movements, dysmorphic features, hypotonia, and arthrogryposis. Molecular testing of the SYNE1 gene confirms diagnosis.
Internationalizing Malawian Higher Education: towards more beneficial academic mobility.
Authors: Banda Lazarus Obed Livingstone , Liu Jin , Munthali George N. Chidimba , Wen Zhou Hui , Mbughi Colleen , Kayembe Helex , Banda Jane Thokozani , Ngulube Peter , Enbeyle Wegayehu 
Published: November 2021

Abstract
Aim: This study investigated the intentions, opportunities, and barriers to engaging in a meaningful internationalization of higher education in Malawi.Methods: This was cross-sectional research that was done between June and October 2021. Using a purposive (judgmental) sampling, we recruited 212 respondents from various institutions of higher education in Malawi. Multilinear regression analysis was used to analyze the factors with the P-value set at 0.05 level of statistical significance. Results: The results indicated that the majority of the respondents were males (63.7%) who fell into 30 years age bracket. Further, the results from the multilinear regression analysis indicate that Institutional collaboration (ß=0.326, p=0.000, CI=0.27—0.383), clear Policy on Mobility (ß=0.146, p=0.0.004, CI=0.047-0.246), experience (ß=0.083, p=0.117, CI=-0.021-0.186), academic rank (ß=0.114, p=0.000, CI=0.069-0.159) were positively statistically significant variables, whereas on the other hand, Occupation (ß=-0.131, p=0.002, CI=-0.213-0.49), academic qualification (ß=-0.106, p=0.013, CI=-0.19-0.023 and mobilityImportance (ß=-0.116, p=0.022, CI=-0.215-0.017) were negatively significant variables respectively.Conclusion and Recommendations: institutions need to invest in international and inter-institutional collaboration, clarify policy direction regarding academic mobility, keep track and linkages with mobile faculty, create a conducive social and formal institutional culture that attracts back mobile faculty, and reduce staff turnover.
Effect of Heated Humidified High Flow Nasal Cannula (HFNC) Oxygen Therapy in Dyspnea Patients with Advanced Cancer, a Randomized Controlled Clinical Trial.
Authors: Xu Zhaoning , Li Pingping , Zhang Chi , Ma Dedong 
Published: November 2021

Abstract
PurposeHeated humidified high flow nasal cannula(HFNC) oxygen therapy is one of the most important oxygen therapy methods, which are commonly applied to relieve dyspnea in advanced cancer patients. Our study aims to observe the efficacy and safety of HFNC oxygen therapy on dyspnea patients with advanced cancer and explore the clinical application.Methods 60 subjects with advanced cancer requiring oxygen therapy from a Grade 3, Class A hospital in China were recruited and randomized (1:1) to traditional nasal catheter oxygen therapy or HFNC. Primary outcomes were dyspnea, oral dryness and sleep condition, which were recorded after 72-hour treatment. Secondary outcomes were heart rate (HR), respiration rate (RR), SpO2, PaO2 and PaCO2, which were recorded after 2, 6, 24 and 72 hours-treatment.Results72 hours after treatment, there were significant improvement in all primary outcomes (P<0.001). PaO2 and RR were statistically changed 2 hours after HFNC treatment (P<0.001). PaCO2 and HR were statistically changed 24 hours after HFNC treatment (P<0.001). ConclusionHFNC oxygen therapy has good effect, high safety and is easy to be accepted by dyspnea patients with advanced cancer. It can be used as the first choice of oxygen therapy for these patients and has broad clinical prospects.This work was retrospectively registered in the Chinese Clinical Trials Registry (ChiCTR2100049582) on August 4, 2021.
Effect of Growth Medium Nitrogen and Phosphorus on Nutritional Composition of Lemna Minor (an Alternative Fish and Poultry Feed)
Authors: Ullah Hafiz , Gul Bakhtiar , Khan Haroon , Akhtar Naveed , Rehman Khushnood Ur , Zeb Umar 
Published: November 2021

Abstract
Duckweed (L. minor) belongs to the family of aquatic macrophytes and grows profoundly on the surface of water reservoirs in polluted areas of Pakistan. The plant can be used as a potential alternative for the fish and poultry industry to comply with the promptly growing demand for feed. Our study investigates the effect of varying concentrations (ppm) of nutrients, like nitrogen (N), phosphorus(P) and their combination (NP) on the carbohydrate, lipid, protein, and mineral (Ca, Mg, Fe, Mn & Zn) contents of the duckweed (L. minor). A substantial effect of varying concentrations of N, P and their combinations was seen on all the above-mentioned parameters. Higher protein, lipid, and carbohydrate contents were recorded for 30ppm NP, 20ppm NP, and 10ppm NP, respectively. Minerals contents (Ca, Mg, Fe, Mn & Zn) increased by 20ppm in the case of phosphorus and all nitrogen concentrations. The combined application of NP was more effective in boosting up the protein, carbohydrate, and lipid content whereas less effective in increasing the mineral contents. The NP combinations affected all other parameters except the mineral contents. A decline in the concentration of (N) and (P) showed a negative correlation with the nutritional composition of the plant.
Fast and sensitive GCaMP calcium indicators for imaging neural populations
Authors: Looger Loren , Zhang Yan , Rózsa Martón , Liang Yajie , Bushey Daniel , Wei Ziqiang , Zheng Jihong , Reep Daniel , Broussard Gerard Joey , Tsang Arthur , Tsegaye Getahun , Narayan Sujatha , Obara Christopher , Lim Jing , Patel Ronak , Zhang Rongwei , Ahrens Misha , Turner Glenn , Wang Samuel , Korff Wyatt , Schreiter Eric , Svoboda Karel , Hasseman Jeremy , Kolb Ilya 
Published: November 2021

Abstract
Calcium imaging with protein-based indicators is widely used to follow neural activity in intact nervous systems. The popular GCaMP indicators are based on the calcium-binding protein calmodulin and the RS20 peptide. These sensors report neural activity at timescales much slower than electrical signaling, limited by their biophysical properties and trade-offs between sensitivity and speed. We used large-scale screening and structure-guided mutagenesis to develop and optimize several fast and sensitive GCaMP-type indicators. The resulting ‘jGCaMP8’ sensors, based on calmodulin and a fragment of endothelial nitric oxide synthase, have ultra-fast kinetics (rise times, 2 ms) and still feature the highest sensitivity for neural activity reported for any protein-based sensor. jGCaMP8 sensors will allow tracking of larger populations of neurons on timescales relevant to neural computation.
Immobilization of Baeyer-Villiger monooxygenase from acetone grown Fusarium sp.
Authors: Takagi Michio , T.sriw Kotchakorn , Masuda Ayaka , Kawaguchi Nozomi , Fukui Shusuke , Lan Huong Le Viet , Kato Dai-ichiro , Kitayama Takashi , Fujii Mikio , Koesoema Afifa Ayu , Matsuda Tomoko 
Published: November 2021

Abstract
ObjectiveA novel biocatalyst for Baeyer–Villiger oxidations is necessary for pharmaceutical and chemical industries, so this study aims to find a Baeyer–Villiger monooxygenase (BVMO) and to improve its stability by immobilization. ResultsAcetone, the simplest ketone, was selected as the only carbon source for the screening of microorganisms with a BVMO. A eukaryote, Fusarium sp. NBRC 109816, with a BVMO ( F BVMO), was isolated from a soil sample. F BVMO was overexpressed in E. coli and successfully immobilized by the organic-inorganic nanocrystal formation method. The immobilization improved the thermostability of F BVMO. Substrate specificity investigation revealed that both free and immobilized F BVMO were found to show catalytic activities not only for Baeyer–Villiger oxidation of ketones to esters but also for oxidation of sulfides to sulfoxides. Furthermore, a preparative scale reaction using immobilized F BVMO was successfully conducted. ConclusionsFBVMO was discovered from an environmental sample, overexpressed in E. coli , and immobilized by the organic-inorganic nanocrystal formation method. The immobilization successfully improved its thermostability.
Low-Pt NiNC-supported PtNi nanoalloy oxygen reduction reaction electrocatalysts—In situ tracking of the atomic alloying process
Authors: Feng Quanchen , Wang Xingli , Klingenhof Malte , Heggen Marc , Strasser Peter 
Published: November 2021

Abstract
Carbon-supported platinum-nickel (Pt-Ni) alloy nanoparticles (NPs) emerge as the electrocatalysts of choice for deployment in polymer electrolyte membrane fuel cell (PEMFC) cathodes. To date, viable PtNi nanoalloy catalysts are characterized by large Pt weight loading of up to 50 wt%. To a large extent, their preparation processes often involve the use of expensive or even hazardous organometallic metal precursors, solvents and capping agents, substantially limiting their synthetic scalability and sustainability. Here, we report a novel synthetic strategy toward highly active low-Pt loaded PtNi nanoalloy Oxygen Reduction Reaction (ORR) catalysts. The synthesis involves the Pyrolysis and Leaching of Ni-organic polymers, subsequent Pt nanoparticle Deposition followed by thermal Alloying (referred to as PLDA) to prepare single Ni atom site (NiNC)-supported bimetallic PtNi nanoalloy electrocatalysts with very low Pt weight contents of 3–5 wt% Pt loading. We demonstrate that despite this low Pt weight loading, the catalysts exhibit more favorable Pt-mass activities compared to conventional, carbon-supported 20–30 wt%Pt Pt-loaded benchmark PtNi alloy catalysts. Using in situ transmission electron microscopy, cyclic voltammetry, and surface CO stripping techniques, we track and unravel the key stages of the formation process of the PtNi nanoparticle catalysts directly at the atomic scale. By carefully chosen reference experiments, we find that carbon-encapsulated Ni NPs, rather than NiNx single sites, serve exclusively as the Ni atom source for PtNi alloy formation during thermal treatments. Our materials concepts offer a pathway to further decrease the overall Pt content of PEM fuel cell devices.
Intestinal Epithelial Cell Derived Exosomes Package MicroRNA-23a-3p Alleviate Gut Damage After Ischemia/Reperfusion via Targeting MAP4K4
Authors: Yang Jin , Zheng Xinguo , Wu Yanling , Wang Aiping , Wang Chenhui , Chen Wenxin , Zhong Shan , Yang Hui 
Published: November 2021

Abstract
Backgroud:
Intestinal epithelial cells (IEC) contribute to regulate gut injury after intestinal isochemia/reperfusion (II/R). Exosomes are well documented to deliver bioactive molecules to recipient cells to modulate cell function. However, the role of IEC-derived exosomes in gut damage after II/R and the underlying mechanisms remains unclear.
Methods
IEC-derived exosomes were intravenously injected into the rats after the superior mesenteric artery occlusion for 45 min following reperfusion for 6h. The scores of gut injury, ZO-1 and IL-17A expression, TNF-α and endotoxin concentration were determined at 3 days after II/R assault. Exosomal microRNA-23a-3p and its downstream target molecule-MAP4K4 were also detected.
Results
Our results showed that the IEC-derived exosomes attenuated the damage of IECs after oxygen-glucose deprivation in vitro (p<0.05) and the degree of gut injury at 3 days after II/R assault in vivo (p<0.05). Injection of miR-23a-3p knockdown exosomes derived from IECs aggravated the II/R injury whereas PF-6260933, a small-molecule inhibitor of MAP4K4, was partly reversed the injuried degree by intraperitoneal injection. Underlying mechanism studies revealed that exosomal miR-23a-3p attenuated gut damage by regulating its downstream target MAP4K4.
Conclusions
We demonstrated that IEC-derived exosomes alleviated the II/R injury and promoted gut damage recovery via exosomal miR-23a-3p and its downstream target molecule-MAP4K4.
miR-93-5p Suppresses Ovarian Cancer Malignancy and Negatively Regulate CCND2 By Binding To Its 3’UTR Region
Authors: Chen Guotong , Yan Yiwei , Qiu Xiaojv , Ye Chengfeng , Jiang Xingmei , Song Shuo , Zhang Yibo , Chang Huanhuan , Wang Leqi , He Xuehuan , Tang Lingrong , Zhang Qingyu , Zhang Ying 
Published: November 2021

Abstract
Ovarian cancer is the most lethal gynecological cancer worldwide, but the underlying mechanism of ovarian cancer malignancy acquirement is largely unknown. miRNA is ubiquitously implicated in disease especially in cancer initiation and progression. In current study, we firstly detected the expression level of miR-93-5p in ovarian cancer patient samples and conducted a survival analysis. Our data revealed miR-93-5p is a favorable prognostic factor but is downregulated in ovarian cancer patients. Secondly, CCK8 assay wound healing assay and flow cytometry-based cell cycle analysis and apoptotic cell analysis were performed respectively to study the function of miR-93-5p. Functional analysis show miR-93-5p promotes ovarian cancer malignancy in term of cell proliferation, migration but reduce cell death. Bioinformatic analysis showed Cyclin-D2(CCND2) is a candidate gene of miR-93-5p with the binding site in its 3’UTR region. Furthermore, quantitive-PCR and western blot were utilized to measure miR-93-5p, CCND2 levels in tissues samples and cell lines. Our data suggested miR-93-5p is negatively correlated to the level of CCND2 mRNA and protein. Finally, Luciferase report assay was conducted, and we demonstrated miR-93-5p reduces CCND2 expression by binding to the 3’UTR region. Our study revealed the function of miR-93-5p in ovarian cancer malignancy and declaimed CCND2 as a target of miR-93-5p.
Parameter design of PLA/Wood Fused Filament Fabrication using Taguchi optimization methodology
Authors: Kechagias John D. , Zaoutsos Stephanos , Chaidas Dimitrios , Vidakis Nectarios 
Published: November 2021

Abstract
This study investigates the effects of four variables during fused filament fabrication of organic biocompatible composite material, PLA with coconut flour, at the ultimate tensile strength and elasticity module of the printed parts. The parameter optimization uses Taguchi L18 design and regression models. The examined deposition variables are the layer thickness, the nozzle temperature, the raster deposition angle, and filament printing speed. The effects of the above variables on the strength of the parts are essential to enhance the mechanical response of the printed parts. The experimental outcomes are investigated using the ANOM and ANOVA analysis and modeled utilizing linear regression models. In addition, an independent experiment was repeated three times at optimum parameters' levels to evaluate the methodology, giving predictions errors less than 3%.
Physical Activity Trajectory In The First 10 Months of The COVID-19 Pandemic in Southern Brazil: a Follow-Up Study
Authors: Caputo Eduardo L , Feter Natan , Leite Jayne S. , Doring Igor R. , Cassuriaga Júlia , Delpino Felipe M , Huckembeck Caroline M. , Alt Ricardo , Silva Marcelo C. , Rombaldi Airton J. , Reichert Felipe F. 
Published: November 2021

Abstract
Background A continuous tracking of the PA level during the COVID-19 pandemic is important to understand how people’s behaviour has varied along time. The aim of this study was to evaluate the physical activity (PA) trajectory over the first 10 months of the COVID-19 pandemic in the south of Brazil. Methods Data from three timepoints of the PAMPA Cohort were used, as follows: 1) pre-COVID-19 (retrospective); 2) Jun-Jul 2020; 3) Dec 2020/Jan 2021. PA practice, frequency, duration, and place where activities were performed (at or out of home) were assessed. Results A reduction in any, sufficient and out of home PA, as well as mean days and minutes were observed from the first (pre-COVID-19) to the second (Jun/Jul 2020) timepoint, followed by an increase in the third timepoint (Dec 2020/Jan 2021). Female participants showed lower PA prevalence for any, sufficient and out of home PA. No marked differences of PA prevalence were observed for income and educational level.Conclusion During the first 10 months of COVID-19 pandemic there was a marked variability on PA pattern in adults from southern Brazil. An ongoing tracking of PA behavior during COVID-19 pandemic is important to understand how this behaviour varies and what actions are needed in order to increase PA on populational level.
Firing Activities in Fractional-Order Hindmarsh-Rose Neuron with Multistable Memristor as Autapse
Authors: Li Zhijun , Xie WenQiang , Zeng Jinfang , Zeng Yicheng 
Published: November 2021

Abstract
Compared with integer order neurons, fractional-order neuron model can more accurately describe the firing behavior of biological neurons. Considering the fact that memristors have the characteristics similar to biological synapses, a fractional-order multistable memristor is firstly proposed in this study. It is verified that the fractional-order memristor has multiple local active regions and multiple stable hysteresis loops, and the influence of fractional order on its nonvolatility is also revealed. Then by considering the fractional-order memristor as an autapse of HR neuron model, a fractional-order memristive neuron model is developed. The effects of the initial value, external excitation current, coupling strength and fractional order on the firing behavior are discussed by time series, phase diagrams, Lyapunov exponents and inter spike interval (ISI) bifurcation diagrams. Three coexisting firing patterns, including irregulate A-periodic bursting, A-periodic bursting and chaotic bursting, dependent on the memristor initial values are observed. It is also revealed that the fractional order can not only induce the transition of firing patterns, but also change the firing frequency of the neuron. Finally, a neuron circuit with variable fractional order is designed to verify the numerical simulations.
Dominance of Zoonotic Pathogen Cryptosporidium Meleagridis in Broiler Chickens in Guangdong, China, Reveals Evidence of Cross-Transmission
Authors: Lin Xuhui , Xin Luyao , Qi Meng , Hou Minyu , Liao Shenquan , Qi Nanshan , Li Juan , Lv Minna , Cai Haiming , Hu Junjing , Zhang Jianfei , Ji Xiangbo , Sun Mingfei 
Published: November 2021

Abstract
Background

Cryptosporidium is one of the most prevalent parasites infecting both birds and mammals. To examine the prevalence of Cryptosporidium species and evaluate the public health significance of domestic chickens in Guangdong Province, Southern China, we analyzed 1001 fecal samples collected from 43 intensive broiler chicken farms from six distinct geographical regions between June 2020 and March 2021.
Methods

Individual DNAs were subjected to nested PCR-based amplification and sequencing of the small subunit of the nuclear ribosomal RNA gene (SSU rRNA). The 60 kDa glycoprotein gene (pgp60) was performed from all positive SSU rRNA samples to characterise subtypes of C. meleagridis.
Results

Cryptosporidium infection rates was found to be 13.2%, comprising with infections with C. meleagridis (78/1001, 7.8%), C. baileyi (48/1001, 4.8%) and mixed infections (6/1001, 0.6%). Three subtype families were identified, IIIb, IIIe and IIIg. Six subtypes were identified in broiler chickens, including one novel (IIIgA25G3R1a) and five previously reported (IIIbA23G1R1c, IIIbA24G1R1, IIIbA21G1R1a, IIIeA17G2R1 and IIIeA26G2R1). Within these subtypes, five known subtypes were genetically identical to those identified in humans.
Conclusions

This is the first report of C. meleagridis in chickens from Guangdong. The frequent occurrence of C. meleagridis in domestic chickens and the common C. meleagridis subtypes identified both in humans and chickens is of public health significance. Our study indicates that broiler chickens represent a potential zoonotic risk for the transmission of Cryptosporidium in this region.
Olorofim and the azoles are antagonistic in A. fumigatus and functional genomic screens reveal mechanisms of cross resistance.
Authors: Rhijn Norman van , Hemmings Sam , Valero Clara , Amich Jorge , Bromley Michael 
Published: November 2021

Abstract
Aspergillosis, in its various manifestations, is a major cause of morbidity and mortality. Very few classes of antifungal have been approved for clinical use to treat these diseases and resistance to the first line therapeutics is increasing. A new class of antifungals, the orotomides, are currently in development with the first compound in this class olorofim in late-stage clinical trials. In this study, we characterise a network of genes that govern olorofim response in A. fumigatus. We reveal that the number of transcription factors that regulate olorofim susceptibility are far fewer than we have previously observed for the azoles and the change in sensitivity observed in these isolates is less extreme. Intriguingly, loss of function in two higher order transcriptional regulators, HapB a member of the heterotrimeric HapB/C/E (CBC) complex or the regulator of nitrogen metabolic genes AreA, leads to cross resistance to both the azoles and olorofim. However, a clinical azole resistant isolate with a point mutation in HapE (hapEP88L) retains sensitivity to olorofim. Our transcriptomic analysis suggests that altered sensitivity to olorofim may emerge via modification of genes involved in the production of pyrimidine biosynthetic precursors. Finally, we also show that the action of the azoles are antagonistic to olorofim in vitro.
Ethylene-Inducible AP2/ERF Transcription Factor Promotes Capsaicinoid Biosynthesis in Capsicum
Authors: deng minghua , zhao kai , lv junheng , zhang xiang , li zuosen , zhang hong , huo jinlong , wan hongjian , wang ziran , zhu haishan , zou xuexiao , wen jinfen 
Published: November 2021

Abstract
Ethylene is very important in the process of plant development and regulates the biosynthesis of a lot of secondary metabolites. In these regulatory mechanisms, transcription factors that mediate ethylene signals play a very important role. Capsaicinoids (CAPs) only synthesized and accumulated in Capsicum species, causing their fruit to have a special pungent taste, which can protect against attack from herbivores and pathogens. In this study, we identified the transcription factor CcERF2, which is induced by ethylene, and demonstrated its regulatory effect on CAPs biosynthesis. Transcriptome sequencing analysis revealed the expression patterns of CcERF2 and multiple genes associated to CAPs biosynthesis were basically the same. The spatiotemporal expression results showed CcERF2 was preferentially expressed in the placenta of the spicy fruit. Ethylene can induce the expression of CcERF2 and CAPs biosynthesis genes (CBGs). CcERF2 gene silenced, 1-MCP and PZA treatments caused a decrease in expression of CBGs and a sharp decrease in content of CAPs. The results indicated CcERF2 was associated with the CAPs biosynthesis.