bluuebunny/crossref_metadata_2025_split huggingface dataset:
Inleiding
Authors: Kaptein Nico , Scheepmaker Marit 
Published: July 2021

The future of DNA analysis
This special issue of Justitiële verkenningen (Judicial Explorations) discusses three developments that have driven the use of DNA to grow: technological advances in DNA data sequencing, the booming market for commercial DNA testing, and the internationalization of the collection and sharing of DNA data. More and more DNA data is being distributed without any insight into what exactly happens to this data. While strict rules apply to the management and use of DNA data by the police and judicial authorities, this is not yet the case for data from commercial DNA tests. In this episode of Justitiële verkenningen, particular attention is paid to the rise of investigative genetic genealogy (IGG). This phenomenon means that the police and the judicial authorities use data from commercial DNA databases to track down suspects. The successes achieved in this way in deadlocked murder cases, including in the United States, are also discussed. It is clear that not everyone who sends DNA material to a DTC company foresees such an application, and this use is therefore controversial. Moreover, relatives of these customers are not systematically informed and they are usually not asked for permission. This special issue aims to contribute to the public debate on the consequences and risks of the dissemination of DNA data.
Het gebruik van DNA in het opsporingsproces
Authors: de Poot Christianne 
Published: July 2021

The use of DNA in the criminal investigation process
This article describes why forensic DNA research is so interesting for criminal investigation processes, and why DNA does not yet play the role in these processes that could be expected given its unique properties. To this end, the bottlenecks that arise in the forensic investigation process are discussed as well as the opportunities to solve these bottlenecks in the coming years with new technologies and new scientific insights. The article focuses on (1) finding biological traces, (2) determining the relevance and the success rate of these traces, (3) the learning process of criminal investigators, (4) the importance of integrating processes that are currently performed in different places by different professionals, and (5) the promises of rapid mobile DNA technologies in this development.
Een goudmijn vol tips
Authors: Meulenbroek Lex , Aben Diederik 
Published: July 2021

A gold mine full of tips. The use of genealogical DNA databases in criminal investigation and identification
The success of investigative genetic genealogy (IGG) in the US hasn’t gone unnoticed in Europe. After US police announced worldwide that the Golden State Killer had been identified with the application of IGG, the Swedish police and judiciary applied the same method to solve a double murder that had remained unsolved for sixteen years. How did this method come about? A young woman unfamiliar with her real name, age, parents, and origins came up with the idea that private genealogical DNA databases that allow customers to trace their distant relatives could also be used to discover her identity. Since then, in the US many cold cases have been solved with the help of these databases and also the identity of many unidentified human remains has been traced. Questions concerning this new method of investigation arise, to which the beginning of an answer is given here. What does the method entail? Is it allowed to use this method in the Netherlands as well?
Genealogische DNA-databanken: consequenties van het delen van ons DNA
Authors: Kaptein Nico 
Published: July 2021

Direct-to-consumer (DTC) genetic testing: consequences of sharing our DNA
This article aims to contribute to the public debate on the consequences and risks of the spreading of DNA data related to direct-to-consumer (DTC) genetic testing. Market developments drive DTC companies to find new business models. As a result of mergers and acquisitions and of the developments of new products and services, DNA data are often used differently than what they were originally collected for. Since DTC DNA data are not protected as well as health-related data generally are, it is hard to keep track of these data. This is partly due to legal and ethical issues such as unclarity of who owns DNA and problems with informed consent. Risks are identified with regards to privacy, information security, the right not to know, (un)equal opportunities, and national security. The author calls for an investment in knowledge and awareness in order to allow for a fair balance between opportunity and risk of DTC DNA products and services.
Consonancias significativas de la escultura pública de Elena Laverón. Poéticas pioneras y analógicas para la construcción social de género
Authors: González Torres Javier 
Published: July 2021

Una conciencia estética crítica con los modelos tradicionales y defensora de la igualdad entre géneros. Este es el punto de partida de la escultura monumental de la artista ceutí Elena Laverón, visible no solamente en el amplio catálogo de sus obras sino en su propia actitud vital; un posicionamiento que le lleva, desde su juventud, a rivalizar con las actuaciones de sus compañeros, consiguiendo vencer reticencias, prejuicios o tratos desiguales.
Este activismo, que no es extraño en la sociedad actual, sí despierta la atención cuando se produce en épocas en las que las miradas androcéntricas prevalecen frente a toda posible resiliencia. Ahí reside el carácter precursor de Laverón al anteceder a planteamientos hoy normalizados y abrir vías experimentales que están en la base de actuaciones transgresoras posteriores. Partiendo de estos parámetros, consigue externalizar un sugestivo ejercicio de resemantización social del espacio público a través de la materia escultórica.
El sustrato último de tales intenciones tiene una especial consonancia con la actitud intelectual planteada décadas antes por la filósofa María Zambrano; los principios de ésta, convertidos en universales, quizá puedan servir de soporte teórico para la actividad artística de Laverón.
An aesthetic conscience, critical of traditional models and defender of gender equality. This is the starting point of the monumental sculpture of the Ceuta artist Elena Laverón, visible not only in the wide catalog of her works but also in her own vital attitude; a position argued from her youth to compete with the actions of her partners and, finally, overcome reluctance, prejudice or unequal treatment. This activism is not strange in today's society but if it arouses attention if it takes place in times where even the androcentric views prevail in the face of any possible resilience. That is the pioneer case of Laverón who, preceding standardized approaches today and opening experimental routes that are the basis of subsequent transgressive actions. Based on these parameters, she manages to externalize a suggestive exercise of social re-semantization of public space through sculptural matter.The ultimate substratum of such intentions is especially in keeping with the intellectual attitude set forth decades earlier by the filosopher María Zambrano; its principles, turned into universal ones, may perhaps serve as theoretical support for Laverón’s artistic activity.
Registrar la memoria desechada. Rastreadores de escombros
Authors: Serrano González Eduardo , De la Torre Oliver Francisco 
Published: July 2021

El presente trabajo analiza cómo la sociedad de consumo contemporánea transforma la ciudad en productora de desechos de memoria abandonados, y cómo estos objetos protagonizan el archivo fotográfico en redes sociales de los denominados rastreadores de desechos. Un fenómeno que estaría cuestionando la construcción de la memoria colectiva desde nuevas perspectivas. Tras esta observación, planteamos afrontar el análisis del objeto desechado como metáfora de la memoria mediante una posible analogía entre estos rastreadores urbanos y el Ángel de la historia de Walter Benjamin. Del mismo modo, vincularemos este fenómeno al cambio de paradigma de lo fotografiable, motivado en gran medida por la popularización de la cámara integrada en dispositivos móviles y las aplicaciones para compartir imágenes. Finalmente, en base a estas claves analizaremos una serie de cuentas en redes sociales donde sus autores desarrollan discursos artísticos alternativos.
The present work analyzes how the contemporary consumer society transforms the city into a producer of abandoned memory waste, and how these objects star in the photographic archive on social networks of the so-called waste trackers. A phenomenon that would be questioning the construction of collective memory from new perspectives. After this observation, we propose to face the analysis of the discarded object as a metaphor for memory by means of a possible analogy between these urban trackers and the Angel of Walter Benjamin's story. In the same way, we will link this phenomenon to the paradigm shift of what can be photographed, largely motivated by the popularization of the integrated camera on mobile devices and image sharing applications. Finally, based on these keys, we will analyze a series of accounts on social networks where their authors develop alternative artistic discourses.
La democracia y el Areópago en la segunda mitad del s. IV a.C.: del Areopagítico de Isócrates al caso de Hárpalo
Authors: Sancho Rocher Laura 
Published: July 2021

In this article I address the question of whether, during the second half of fourth century B.C., the Areopagus was an institution opponent to democracy and, moreover, a refuge of Philip’s supporters or of the oligarchy. I will analyse our sources from Isocrates’ Areopagiticus (358 B.C.) to the Greek defeat during the Lamian War. Evidences lead to conclude that the Areopagus was an important advisory body, always subordinated to the Assembly, that was taken into account in critical moments when deciding to save the city.
NLRP3 inflammasome activation mediates sleep deprivation-induced pyroptosis in mice
Authors: Fan Kun , Yang Jiajun , Gong Wen-Yi , Pan Yong-Chao , Zheng Peibing , Yue Xiao-Fang 
Published: July 2021

Background
Sleep deprivation (SD) has many deleterious health effects, including cognitive decline, work ability decline, inadequate alertness, etc. Neuroinflammation plays an important role in sleep deprivation. However, the underlying mechanism is still unclear.

Methods
In the present study, we detected the activation of microglia and apoptosis of nerve cells in sleep deprivation (SD) mice model using IHC, HE staining and TUNEL apoptosis assay. RT-PCR array data were used to detect the expression of inflammatory bodies in hippocampal CA1 region after sleep deprivation, to explore how NLRP3 inflammasome regulates neuronal apoptosis and how specific signaling pathways are involved in SD-induced activation of NLRP3/pyrosis axis.

Results
We found the number of microglia significantly increased in SD mice, while this effect was blocked by sleep recovery. RT-PCR array data suggested that NLRP3 inflammasome, but not other inflammasomes, was obviously increased in hippocampus CA1 region after sleep deprivation. Mechanistically, we found that NLRP3 mediated the pyroptosis of neurocyte through GSDMD-dependent way , and P38 and ERK-MAPK signaling pathway is involved in SD-induced activation of NLRP3/pyroptosis axis. All these results suggested that MAPK/NLRP3 axis mediated SD-induced pyroptosis.

Conclusion
NLRP3 plays an important role in SD-induced neuroinflammation. Thus, NLRP3 inflammasome is expected to be a potential therapeutic target for SD-induced neuroinflammation.
Study of Effect of Nitrosamine Stress in Progression of Leprosy
Authors: Kadam Anita D. , Khopade Dipali M. 
Published: July 2021

Objective: In several countries, including India, leprosy is an older disease & till now continues to be an important health issue. Leprosy is a chronic granulomatous disease, the contributing agent for this is mycobacterium leprae. Nitric oxide (NO) plays a significant role in immunity to fight against bacteria. But increased NO can harm host tissue by causing altered structure of thiol-containing compounds, which is a significant event that influences the pathogenesis of leprosy. Considering this, the study was planned to determine the type of relationship between NO, nitrothiols, and thiols as disease advances.
Methods: 50 newly diagnosed leprosy patients & 50 healthy controls were included in the study. In the leprosy group,16 were Paucibacillary (PB), and 34 were Multibacillary (MB) type, leprosy patients.
Results: Serum NO and serum nitrothiol significantly increased (p<0.01) in leprosy patients than controls. Further, Serum NO and serum nitrothiol significantly increased (p<0.01) in MB leprosy patients than PB leprosy patients. On the other hand, we found a significantly decreased (p<0.01) level of total thiols in leprosy patients compared with controls. Serum thiols significantly decreased (p<0.01) in MB leprosy patients than PB leprosy patients. Among Both leprosy patients, the negative correlation of NO with thiol and nitrothiol with thiols was observed.
Conclusion: Thus, in leprosy, increased nitric oxide causes modification of thiol groups of proteins and impaired their activity which may be responsible for the severity of disease; hence therapy aimed to reduce nitrosative modification of proteins leprosy.
Acute Polyhydromnios: A Rare Entity
Authors: Chaurasia Tanvi , Shrivastava Deepti , Agarwal Himanshi , Chaurasia Geeta 
Published: July 2021

Acute Polyhydramnios is defined as accumulation of amniotic fluid rapidly complicating approximately 0.2 TO 1.6% of all pregnancies, whereas gestational diabetes (GD) is glucose intolerance in pregnant women, a major health issue in pregnant women. The prevalence rate of glucose intolerance in pregnant women is between 4.6%–14% in well developed areas and 1.7%–13.2% in rural areas. Pregnancies conceived by in vitro fertilisation (IVF) have been associated with higher risk of gestational diabetes mellitus (11.7%), gestational hypertension (9.3%), preeclampsia (4.0%), ICP (11.8%), placenta previa (7.1%), placental abruption (1.6%), PROM (9.9%), placental adherence (7.7%), postpartum haemorrhage (7.3%), and polyhydramnios (2.2%), as compared to spontaneously conceived pregnancies.
Case Description: A 35 years primigravida, 33 weeks gestational age with IVF conception with preeclampsia with breech came with pain in abdomen since 2-3days.On clinical examination, polyhydramnios was diagnosed. On admission she presented with deranged sugar level which was abnormal and sudden rise in Glycosylated hemoglobin seen. Patient was managed conservatively by termination of pregnancy and successful outcome of pregnancy was obtained despite of liquor more than adequate and deranged sugar levels.
Objectives: We examined the routine antenatal profile, radiological changes as well as doppler changes in IVF conceived pregnancies and further management on the basis of clinical, haematological and radiological investigations will be planned accordingly.
Conclusion: Acute polyhydramnios and Gestational diabetes is common in patients conceived through in vitro fertilisation and is associated with poor foe to maternal outcome. Regular antenatal investigations and radiological investigations done to keep her pregnancy in check and reduce the long term effects by modifying treatment modality and by following healthy lifestyles in such patients.
Vesicle capture by membrane‐bound Munc13‐1 requires self‐assembly into discrete clusters
Authors: Li Feng , Kalyana Sundaram Ramalingam Venkat , Gatta Alberto T. , Coleman Jeff , Ramakrishnan Sathish , Krishnakumar Shyam S. , Pincet Frederic , Rothman James E. 
Published: July 2021

Munc13‐1 is a large banana‐shaped soluble protein that is involved in the regulation of synaptic vesicle docking and fusion. Recent studies suggest that multiple copies of Munc13‐1 form nano‐assemblies in active zones of neurons. However, it is not known whether such clustering of Munc13‐1 is correlated with multivalent binding to synaptic vesicles or specific plasma membrane domains at docking sites in the active zone. The functional significance of putative Munc13‐1 clustering is also unknown. Here, we report that nano‐clustering is an inherent property of Munc13‐1 and is indeed required for vesicle binding to bilayers containing Munc13‐1. Purified Munc13‐1 protein reconstituted onto supported lipid bilayers assembled into clusters containing from 2 to ˜ 20 copies as revealed by a combination of quantitative TIRF microscopy and step‐wise photobleaching. Surprisingly, only clusters containing a minimum of 6 copies of Munc13‐1 were capable of efficiently capturing and retaining small unilamellar vesicles. The C‐terminal C2C domain of Munc13‐1 is not required for Munc13‐1 clustering, but is required for efficient vesicle capture. This capture is largely due to a combination of electrostatic and hydrophobic interactions between the C2C domain and the vesicle membrane.
Filtering‐based recursive least squares estimation approaches for multivariate equation‐error systems by using the multiinnovation theory
Authors: Ma Ping , Wang Lei 
Published: July 2021

SummaryThis article researches the filtering‐based parameter estimation issues for a class of multivariate control systems with colored noise. A filtering‐based recursive generalized extended least squares algorithm is derived, in which the data filtering technique is used for transforming the original system into two subidentification systems and the least squares principle is used for estimating parameters of these two subsystems. Furthermore, in order to improve the parameter estimation accuracy, the multiinnovation theory is added for deducing a filtering‐based multiinnovation recursive generalized extended least squares algorithm. The numerical example confirms that these two proposed algorithms are effective.
Substance P/Heparin‐Conjugated PLCL Mitigate Acute Gliosis on Neural Implants and Improve Neuronal Regeneration via Recruitment of Neural Stem Cells
Authors: Kim Donghak , Park DoYeun , Kim Tae Hee , Chung Justin J. , Jung Youngmee , Kim Soo Hyun 
Published: July 2021

AbstractThe inflammatory host tissue response, characterized by gliosis and neuronal death at the neural interface, limits signal transmission and longevity of the neural probe. Substance P induces an anti‐inflammatory response and neuronal regeneration and recruits endogenous stem cells. Heparin prevents nonspecific protein adsorption, suppresses the inflammatory response, and is beneficial to neuronal behavior. Poly(l‐lactide‐co‐ε‐caprolactone) (PLCL) is a soft and flexible polymer, and PLCL covalently conjugated with biomolecules has been widely used in tissue engineering. Coatings of heparin‐conjugated PLCL (Hep‐PLCL), substance P‐conjugated PLCL (SP‐PLCL), and heparin/substance P‐conjugated PLCL (Hep/SP‐PLCL) reduced the adhesion of astrocytes and fibroblasts and improved neuronal adhesion and neurite development compared to bare glass. The effects of these coatings are evaluated using immunohistochemistry analysis after implantation of coated stainless steel probes in rat brain for 1 week. In particular, Hep/SP‐PLCL coating reduced the activation of microglia and astrocytes, the neuronal degeneration caused by inflammation, and indicated a potential for neuronal regeneration at the tissue–device interface. Suppression of the acute host tissue response by coating Hep/SP‐PLCL could lead to improved functionality of the neural prosthesis.
Highly Efficient Far‐Red/NIR‐Absorbing Neutral Ir(III) Complex Micelles for Potent Photodynamic/Photothermal Therapy
Authors: Liu Bingqing , Jiao Jian , Xu Wan , Zhang Miya , Cui Peng , Guo Zhengqing , Deng Yibin , Chen Huabing , Sun Wenfang 
Published: July 2021

AbstractA critical issue in photodynamic therapy (PDT) is inadequate reactive oxygen species (ROS) generation in tumors, causing inevitable survival of tumor cells that usually results in tumor recurrence and metastasis. Existing photosensitizers frequently suffer from relatively low light‐to‐ROS conversion efficiency with far‐red/near‐infrared (NIR) light excitation due to low‐lying excited states that lead to rapid non‐radiative decays. Here, a neutral Ir(III) complex bearing distyryl boron dipyrromethene (BODIPY‐Ir) is reported to efficiently produce both ROS and hyperthermia upon far‐red light activation for potentiating in vivo tumor suppression through micellization of BODIPY‐Ir to form “Micelle‐Ir”. BODIPY‐Ir absorbs strongly at 550–750 nm with a band maximum at 685 nm, and possesses a long‐lived triplet excited state with sufficient non‐radiative decays. Upon micellization, BODIPY‐Ir forms J‐type aggregates within Micelle‐Ir, which boosts both singlet oxygen generation and the photothermal effect through the high molar extinction coefficient and amplification of light‐to‐ROS/heat conversion, causing severe cell apoptosis. Bifunctional Micelle‐Ir that accumulates in tumors completely destroys orthotopic 4T1 breast tumors via synergistic PDT/photothermal therapy (PTT) damage under light irradiation, and enables remarkable suppression of metastatic nodules in the lungs, together without significant dark cytotoxicity. The present study offers an emerging approach to develop far‐red/NIR photosensitizers toward potent cancer therapy.
Revealing the Correlation of OER with Magnetism: A New Descriptor of Curie/Neel Temperature for Magnetic Electrocatalysts
Authors: Li Xiaoning , Bai Ying , Cheng Zhenxiang 
Published: July 2021

AbstractDeveloping accurate descriptors for oxygen evolution reaction (OER) is of great significance yet challenging, which roots in and also boosts the understanding of its intrinsic mechanisms. Despite various descriptors are reported, it still has limitations in the facile prediction, given that complicated analytical techniques as well as time‐consuming modeling and calculations are indispensable. In the present work, strong correlation of magnetic property with OER performance is revealed by in‐depth investigations on the crystal and electronic structures. A facile descriptor of Curie/Neel temperature (TC/N) is developed for La2−xSrxCo2O6−δ perovskite oxides, based on the inference that both magnetism and OER are rooted in the electron exchange interaction. Specifically, both the TC/N and OER activity are proportional to the degree of p‐d orbital hybridization, which increases with enlarged bond angle of Co─O─Co and/or increased oxidation of Co. This finding reveals that TC/N from magnetic characterizations is an effective descriptor in designing novel OER electrocatalysts, and interdisciplinary researches are advantageous for revealing the controversial mechanisms of OER process.