bluuebunny/crossref_metadata_2025_split huggingface dataset:
Successful live birth in a Chinese woman with P450 oxidoreductase deficiency through frozen-thawed embryo transfer: a case report with review of the literature
Authors: Pan Ping , Zheng Lingyan , Chen Xiaoli , Huang Jia , Yang Dongzi , Li Yu 
Published: January 2021

Abstract
Background: Congenital adrenal hyperplasia (CAH) caused by P450 oxidoreductase deficiency (PORD) in 46,XX patients is characterized by genital ambiguity, primary amenorrhea, absent or incomplete sexual maturation, infertility, skeletal malformations and so on. But few pregnancies have been reported from these female patients with PORD. Case Description: A 29-year-old Chinese woman with PORD due to the compound heterozygous mutation (c.1370G>A/c.1196_1204del) in the P450 oxidoreductase(POR) gene had suffered from primary amenorrhea and infertility. She had one cancelled cycle of ovulation induction due to low serum estradiol(E2), high progesterone(P) levels and thin endometrium，then in vitro fertilization (IVF) was recommended. At the first IVF cycle, 4 oocytes were retrieved and 4 viable embryos were cryopreserved due to thin endometrium associated with low E2 and prematurely elevated P after ovarian stimulation, even though oral dexamethasone were used to control adrenal P overproduction at the same time. When basal P fell to <1.5ng/ml after the therapy of oral dexamethasone, artificial endometrial preparation and frozen embryo transfer were performed, resulting in a twin pregnancy. She delivered a healthy boy and a healthy girl by caesarean section at 37 weeks and 2 days of gestation. After the literature search in PORD women, no spontaneous pregnancy has been reported and only two previous case reports of 3 successful pregnancies through IVF were summarized.Conclusions: It is the third report that successful pregnancy was achieved in a CAH woman caused by a compound heterozygous POR mutation, with primary amenorrhea and disorders of steroidogenesis. It seemed that disorders of steroidogenesis caused by PORD didn’t impair the developmental potential of oocytes. IVF and frozen embryo transfer after adequate hormonal control and endometrial preparation should be an effective infertility treatment for PORD women.
Loco-regional recurrence trend and prognosis in young women with breast cancer according to molecular subtypes ： analysis of 1099 cases
Authors: Li Yang , Lu Su , Zhang Yuhan , Wang Shuaibing , Liu Hong 
Published: January 2021

Abstract
BackgroundThe number of young patients diagnosed with breast cancer is on the rise. We studied the rate trend of local recurrence (LR) and regional recurrence (RR) in young breast cancer (YBC) patients and outcomes among these patients based on molecular subtypes. Methods A retrospective cohort study was conducted based on data from Tianjin Medical University Cancer Institute and Hospital for patients ≤35 years of age with pathologically-confirmed primary invasive breast cancer surgically treated between 2006 and 2014. Patients were categorized according to molecular subtypes on the basis of hormone receptor (HR) and human epidermal growth factor receptor 2 (HER2) status. The 5-year rates for LR, RR, and distant metastases (DM) were estimated by Kaplan-Meir statistics. Nelson-Aalen cumulative-hazard plots were used to describe local recurrence- and distant metastases-free intervals. ResultsWe identified 25,284 patients with a median follow-up of 82 months, of whom 1099 (4.3%) were YBC patients ≤35 years of age. The overall 5-year LR, RR and DM rates in YBC patients were 6.7%, 5.1%, and 16.6%, respectively. The LR and RR rates demonstrated a decreasing trend over time (P=0.028 and P=0.015, respectively). We found that early-stage breast cancer and less lymph node metastases increased over time (P=0.004 and P=0.007, respectively). Patients with HR-/HER2+ status had a significantly higher LR (HR 20.4; 95% CI,11.8-35.4) and DM (HR 37.2; 95% CI, 24.6-56.3) at 10 years. Breast conserving surgery (BCS) or mastectomy did not influence rates of LR and RR. In the overall population, the 5-year survival of YBC patients exceeded 90%. ConclusionsThe rates of LR and RR with YBC patients demonstrated a downward trend and the proportion of early-stage breast cancer increased between 2006 and 2014. We report the highest LR rates in this young population were associated with HR-/HER2+ tumors in absence of standard treatment.
External Stenting of Vein Grafts in Coronary Artery Bypass Grating: Interim Results from a Two Centers Prospective Study
Authors: Weltert Luca , Audisio Katia , Bellisaro Alessandro , Bardi Gianluca , Flocco Roberto , De Paulis Ruggero , Centofanti Paolo 
Published: January 2021

Abstract
Background – previous studies evaluating external stents for saphenous vein grafts (SVG) in CABG were limited to on-pump isolated CABG and single grafting technique with one external stent per patient. The objective of this prospective study was to evaluate the safety and the short-term performance of external stents in heterogeneous group of patients which underwent on and off-pump CABG, single and sequential grafting.Methods - 102 patients undergoing CABG were enrolled in two centers. All patients received internal mammary artery to the left anterior descending artery and additional arterial and/or venous grafts. In each patient, at least one SVG was supported with an external stent. Grafts’ patency and SVG lumen uniformity were assessed using CT angiography at a pre-defined time window of 6-12 months post procedure. All patients were prospectively followed-up via phone call and/or visit every 6 months for Major Adverse Cardiac and Cerebrovascular Events.Results - 51 patients (50%) underwent off-pump CABG and 23 patients (23%) were grafted with bilateral internal mammary arteries. Each patient received one or more SVG grafted in a sequential technique (44%) or as single graft (56%). All SVG were externally stented in 84% of patients and in 16% (n=16) one SVG was stented and one remained unsupported. At 6-12 months, patency rates of LIMA, RIMA, externally stented SVG and none-stented SVG were 100%, 100%, 98% and 87.5% respectively. 90% of the externally stented SVG had uniform lumen compared to 37% of the non-stented SVG. Clinical follow-up was completed for all patients with a mean duration of 20 months (range 6-54 months). During follow up period, one patient experienced myocardial infarction due to occlusion of the LIMA-LAD graft and one patient experienced transient ischemic attack. Conclusions - External stenting of SVG is feasible and safe in CABG setting which includes off pump CABG and sequential SVG grafting and associated with acceptable early patency rates.Study registration- Study was registered at ClinicalTrials.gov. NCT01860274 (initial release 20.05.2013)
Strategies for involving patients and the public in scaling-up initiatives in health and social services: study protocol for a scoping review and Delphi survey
Authors: Charif Ali Ben , Plourde Karine V. , Guay-Belanger Sabrina , Zomahoun Hervé Tchala Vignon , Gogovor Amédé , Straus Sharon , Beleno Ron , Kastner Kathy , McLean Robert K.D. , Milat Andrew J. , Wolfenden Luke , Paquette Jean-Sébastien , Geiger Friedemann , Légaré France , Network The RePOS 
Published: January 2021

Abstract
Background: The scale-up of evidence-based innovations is required to reduce waste and inequities in health and social services (HSS). However, it often tends to be a top-down process initiated by policy-makers, and the values of the intended beneficiaries are forgotten. Involving multiple stakeholders including patients and the public in the scaling-up process is thus essential but highly complex. We propose to identify relevant strategies for meaningfully and equitably involving patients and the public in the science and practice of scaling up in HSS.Methods: We will adapt our overall method from the RAND/UCLA Appropriateness Method. Following this, we will perform a two-prong study design (knowledge synthesis and Delphi study) grounded in an integrated knowledge translation approach. This approach involves extensive participation of a network of stakeholders interested in patient and public involvement (PPI) in scaling up and a multidisciplinary steering committee. We will conduct a systematic scoping review following the methodology recommended in the Joanna Briggs Institute Reviewers Manual. We will use the following eligibility criteria: 1) Participants – any stakeholder involved in creating or testing a strategy for PPI; 2) Intervention – any PPI strategy proposed for scaling-up initiatives; 3) Comparator – no restriction; 4) Outcomes: any process or outcome metrics related to PPI; and 5) Setting – HSS. We will search electronic databases (e.g., Medline, Web of Science, Sociological Abstract) from inception onwards, hand searching relevant websites, screen the reference lists of included records, and consult experts in the field. Two reviewers will independently select and extract eligible studies. We will summarize data quantitatively and qualitatively and report results using the PRISMA extension for Scoping Reviews (PRISMA-ScR) checklist. We will conduct an online Delphi survey to achieve consensus on the relevant strategies for PPI in scaling-up initiatives in HSS. Participants will include stakeholders from low-, middle-, and high-income countries. We anticipate that three rounds will allow an acceptable degree of agreement on research priorities. Discussion: Our findings will advance understanding of how to meaningfully and equitably involve patients and the public in scaling-up initiatives for sustainable HSS.Registration: We registered this protocol with the Open Science Framework on August 19, 2020 (https://osf.io/zqpx7/).
A Novel Experimental Porcine Model to Assess the Impact of Differential Pulmonary Blood Flow on Ischemia-reperfusion Injury After Unilateral Lung Transplantation
Authors: Frick Anna Elisabeth , Orlitová Michaela , Vanstapel Arno , Ordies Sofie , Claes Sandra , Schols Dominique , Heigl Tobias , Kaes Janne , Saez-Gimenez Berta , Vos Robin , Verleden Geert M. , Vanaudenaerde Bart , Verleden Stijn E. , Raemdonck Dirk E. Van , Neyrinck Arne P. 
Published: January 2021

Abstract
Background Primary graft dysfunction (PGD) remains a major obstacle after lung transplantation. Ischemia-reperfusion injury is a known contributor to the development of PGD following lung transplantation. We developed a novel approach to assess the impact of increased pulmonary blood flow in a large porcine single-left lung transplantation model.Materials Twelve porcine left lung transplants were divided in two groups (n = 6, in low (LF) and high flow (HF) group). Donor lungs were stored for 24 hours on ice, followed by left lung transplantation. In the HF group, recipient animals were observed for 6h after reperfusion with partially clamping right pulmonary artery to achieve a higher flow (target flow 40 – 60% of total cardiac output) to the transplanted lung compared to the LF group, where the right pulmonary artery was not clamped.Results Survival at 6 hours was 100% in both groups. Histological, functional and biological assessment did not significantly differ between both groups during the first 6 hours of reperfusion. injury was also present in the right native lung and showed signs compatible with the pathophysiological hallmarks of ischemia-reperfusion injury.Conclusions Partial Clamping native pulmonary artery in large animal lung transplantation setting to study the impact of low versus high pulmonary flow on the development of ischemia reperfusion is feasible. In our study, differential blood flow had no effect on IRI. However, our findings might impact future studies with extra-corporeal devices and represents a specific intra-operative problem during bilateral sequential single lung transplantation.
Role of human Pegivirus infections in whole Plasmodium falciparum sporozoite vaccination and controlled human malaria infection in African volunteers
Authors: Tumbo Anneth-Mwasi , Schindler Tobias , Dangy Jean-Pierre , Orlova-Fink Nina , Bieri Jose Raso , Mpina Maximillian , Milando Florence A. , Juma Omar , Hamad Ali , Nyakarungu Elizabeth , Chemba Mwajuma , Mtoro Ali , Ramadhan Kamaka , Olotu Ally , Makweba Damas , Mgaya Stephen , Stuart Kenneth , Perreau Matthieu , Stapleton Jack T. , Jongo Said , Hoffman Stephen L. , Tanner Marcel , Abdulla Salim , Daubenberger Claudia 
Published: January 2021

Abstract
Background: Diverse vaccination outcomes and protection levels among different populations pose a serious challenge to the development of an effective malaria vaccine. Co-infections are among many factors associated with immune dysfunction and sub-optimal vaccination outcomes. Chronic, asymptomatic viral infections can contribute to the modulation of vaccine efficacy through various mechanisms. Human Pegivirus-1 (HPgV-1) persists in immune cells thereby potentially modulating immune responses. We investigated whether Pegivirus infection influences vaccine-induced responses and protection in African volunteers undergoing whole P. falciparum sporozoites-based malaria vaccination and controlled human malaria infections (CHMI). Methods: HPgV-1 prevalence was quantified by RT-qPCR in plasma samples of 96 individuals before, post vaccination with PfSPZ Vaccine and after CHMI in cohorts from Tanzania and Equatorial Guinea. The impact of HPgV-1 infection was evaluated on (1) systemic cytokine and chemokine levels measured by Luminex, (2) PfCSP-specific antibody titers quantified by ELISA, (3) asexual blood-stage parasitemia pre-patent periods and parasite multiplication rates, (4) HPgV-1 RNA levels upon asexual blood-stage parasitemia induced by CHMI.Results: The prevalence of HPgV-1 was 29.2% (28/96) and sequence analysis of the 5' UTRand E2 regions revealed the predominance of genotypes 1, 2 and 5. HPgV-1 infection was associated with elevated systemic levels of IL-2 and IL-17A. Comparable vaccine-induced anti-PfCSP antibody titers, asexual blood-stage multiplication rates and pre-patent periods were observed in HPgV-1 positive and negative individuals. However, a tendency for higher protection levels was detected in the HPgV-1 positive group (62.5%) compared to the negative one (51.6%) following CHMI. HPgV-1 viremia levels were not significantly altered after CHMI. Conclusions: HPgV-1 infection did not alter PfSPZ Vaccine elicited levels of PfCSP-specific antibody responses and parasite multiplication rates. Ongoing HPgV-1 infection appears to improve to some degree protection against CHMI in PfSPZ-vaccinated individuals. This is likely through modulation of immune system activation and systemic cytokines as higher levels of IL-2 and IL17A were observed in HPgV-1 infected individuals. CHMI is safe and well tolerated in HPgV-1 infected individuals. Identification of cell types and mechanisms of both silent and productive infection in individuals will help to unravel the biology of this widely present but largely under-researched virus.
Evidence of neuroplasticity with robotic hand exoskeleton study for post-stroke rehabilitation: a randomized controlled trial
Authors: Singh Neha , Saini Megha , Kumar Nand , Srivast M.V. Padma , Mehndiratta Amit 
Published: January 2021

Abstract
Background: A novel electromechanical robotic-exoskeleton was designed in-house for the rehabilitation of wrist joint and Metacarpophalangeal (MCP) joint. Objective: The objective was to compare the rehabilitation effectiveness (clinical-scales and neurophysiological measures) of robotic-therapy training sessions with dose-matched \conventional therapy in patients with stroke.Methods: A pilot prospective parallel randomized controlled study at clinical-settings was designed with patients with stroke within 2 years of chronicity. Patients were randomly assigned to receive an intervention of 20 sessions of 45 minutes each, five days a week for four weeks, in Robotic-therapy Group (RG) (n=12) and conventional upper-limb rehabilitation in Control-Group (CG) (n=11). We hypothesized to evaluate the exoskeleton based therapy for the effects on the functionality of upper-limb and cortical-excitability in patients with stroke as compared to conventional rehabilitation. Clinical-scales– Modified Ashworth Scale, Active Range of Motion, Barthel Index, Brunnstrom Stage and Fugl-Meyer scale, and neurophysiological measures of cortical-excitability (using Transcranial Magnetic Stimulation) –Motor Evoked Potential and Resting Motor threshold, were acquired pre and post-therapy. No side effects were noticed in any of the patients. Results: Both RG and CG showed significant (p<0.05) improvement in all clinical motor-outcomes except Modified Ashworth Scale in CG. RG showed significantly (p<0.05) higher improvement over CG in Modified Ashworth Scale, Active Range of Motion, and Fugl-Meyer (FM) scale and FM Wrist-/Hand component. An increase in cortical-excitability in ipsilesional-hemisphere was found to be statistically significant (p<0.05) in RG over CG, as indexed by a decrease in Resting Motor Threshold and increase in the amplitude of Motor Evoked Potential. No significant changes were shown by the contralesional-hemisphere. Interhemispheric RMT-asymmetry evidenced significant (p<0.05) changes in RG over CG indicating increased cortical-excitability in ipsilesional-hemisphere along with interhemispheric changes. Conclusion: Robotic-exoskeleton training appears to be benificial for improving motor-outcomes and cortical-excitability in patients with stroke. Neurophysiological changes in RG could most likely be a consequence of plastic reorganization and use-dependent plasticity. Trial Registry Number: ISRCTN95291802
Anti-anxiety Properties of Selected Medicinal Plants
Authors: Khan Asmatullah , Akram Muhammad , Thiruvengadam Muthu , Daniyal Muhammad , Zakki Shahbaz Ahmad , Munir Naveed , Zainab Rida , Heydari Mojtaba , Mosavat Seyed Hamdollah , Rebezov Maksim , Shariati Mohammad Ali 
Published: January 2021

:Exploration of new drugs targeting anxiety treatment is a major concern worldwide. Medicinal plants are being used as a potential source of novel drugs for anxiety disorders. The objective of this review is to provide information about the healing outcomes of anxiety treatment with natural products. Valeriana officinalis, Citrus aurantium, Commelina benghalensis, Achyranthes aspera, Mimosa pudica, Achillea millefolium, Nymphaea alba, Leonurus cardiac, Camellia sinensis, Turnera aphrodisiaca, Crataegus oxyacantha and Piper methysticum showed promising effects on anxiety in animal models. In clinical studies, passion flower, kava, valerian, St John's wort, and ashwagandha showed the most positive results. More studies are needed for the exploration of the anti-anxiety of medicinal plants. In drugs derived from natural sources have explored many components that are playing an essential role in curing anxiety disorders and associated complications.
Insight View on Synthetic Strategies and Biological Applications of Pyrimidobenzothiazoles
Authors: Agarwal Dinesh Kumar , Sahiba Nusrat , Sethiya Ayushi , Soni Jay , Teli Pankaj , Agarwal Shikha , Goyal Pradeep Kumar 
Published: January 2021

:
Pyrimidobenzothiazoles and its derivatives are important structural scaffolds in the field of synthetic organic chemistry and medicinal chemistry. The review is focused on one pot multicomponent approaches for generating pyrimidobenzothiazoles and its derivatives along with their interesting biological profile over the last ten years. Several eco-benign and cost effective approaches such as nano catalyst, ionic liquid, metal, solid acid, catalyst free, solvent free, etc. for its synthesis have been discussed in this review. Different synthetic strategies of pyrimidobenzothiazole derivatives have been compiled and discussed using 2-aminobenzothiazole, 1,3 diketones, substituted aldehydes, alkynoic acid, bis-methyl thiomethylene malononitrile, Baylis Hillman acetates, etc.
Delayed Multiple Non-ischemic Cerebral Enhanced Lesions After Endovascular Therapy For Left Internal Carotid Aneurysm: A Case Report
Authors: Cai Yanli , Huang Lin , Hao Jianqiang , Xie Fei , Ling Tianjin , Richard Seidu A. 
Published: January 2021

Introduction:
Endovascular therapy (EVT) for intracranial arterial aneurysms is depicted
with several complications. Very recently, delayed non-ischemic cerebral enhanced (NICE) lesions
have been identified as a rare complication associated with EVT. This complication always
stands a higher chance of being missed in asymptomatic patients. We report a case of multiple
NICE lesions in a known chronic hepatitis B infection and chronic gastritis patient with left internal
carotid aneurysm (ICA) treated with detachable coils.

Case Presentation:
A 52 years old female with left ICA was treated with detachable coils via the
endovascular route. Three weeks after the operation, she presented with numbness of her right
limbs which was persistent and waked her up from sleep each night. She admitted skin allergies after
wearing metals except for gold and silver since childhood. MRI revealed multiple abnormal lesions
in the left temporal lobe, hippocampus, insula, and parietal lobe and some perifocal edema
which were consistent with the diagnosis of delayed NICE lesions.

Conclusion:
It is very important to report the occurrences of these lesions in literature because of
their allergic origin. We advocate that allergy to metals especially those used in coating endovascular
equipment should be evaluated before every EVT for intracranial aneurysms.
A Sentiment Analysis Based Approach for Customer Segmentation
Authors: Bhatia Madhulika , Bhatnagar Anisha 
Published: January 2021

Background:
Customer Segmentation is the process of dividing customers into groups based on some
demographic factors in order to get an idea of the targeted audience for a product and to best market said product.

Objective:
Sentiment Analysis on customer reviews is one way that this process can be enhanced to get not just
demographic information but subjective information and preferences as well.

Methods:
In this study, Long Short-Term Memory model, a deep learning technique has been applied for Sentiment
Analysis and its results have been used to perform Customer Segmentation on demographic data containing information
such as age and gender. Segmentation was performed using Spectral Clustering. Cluster Labels were extracted to perform
supervised classification using different supervised algorithms, such as Support Vector Machines, Random Forests,
Decision Trees and Logistic Regression.

Results:
An accuracy of 90.9% was achieved by the LSTM model. An accuracy of 100% was achieved by the Random
Forest and Decision Tree Classifiers.