bluuebunny/crossref_metadata_2025_split huggingface dataset:
An optimized support vector machine for lung cancer classification system
Authors: Oyediran Mayowa O. , Ojo Olufemi S. , Raji Ibrahim A. , Adeniyi Abidemi Emmanuel , Aroba Oluwasegun Julius 
Published: December 2024

IntroductionLung cancer is one of the main causes of the rising death rate among the expanding population. For patients with lung cancer to have a higher chance of survival and fewer deaths, early categorization is essential. The goal of thisresearch is to enhance machine learning to increase the precision and quality of lung cancer classification.MethodsThe dataset was obtained from an open-source database and was utilized for testing and training. The suggested system used a CT scan picture as its input image, and it underwent a variety of image processing operations, including segmentation, contrast enhancement, and feature extraction.ResultsThe training process produces a chameleon swarm-based supportvector machine that can identify between benign, malignant, and normal nodules.ConclusionThe performance of the system is evaluated in terms of false-positive rate (FPR), sensitivity, specificity, recognition time and recognition accuracy.
Evaluating the prognostic relevance of neutrophil-to-lymphocyte ratio in cervical cancer: a systematic review and meta-analysis
Authors: Zhuang Xieyan , Li Yan , Zheng Hongfeng , Fu Langjing 
Published: December 2024

BackgroundRecently, the neutrophil-to-lymphocyte ratio (NLR) has emerged as a promising prognostic marker for survival outcomes in individuals affected cervical cancer. However, research specifically focusing on the prognostic relevance of NLR across different cancer stages and in cases of recurrent metastases remains scant.MethodsWe executed a systematic review of the literature from databases including PubMed, Embase, the Cochrane Library, and Web of Science, covering publications up to March 3, 2024. Studies evaluating the relationship between NLR and patient clinical outcomes were retrieved, guided by specifically defined inclusion and exclusion parameters. The key goals were to assess progression-free survival (PFS) and overall survival (OS), measured through hazard ratios (HR) and 95% confidence intervals (CI).ResultsThis meta-analysis encompassed 38 retrospective cohort studies, including data from 10,246 patients. The aggregated data showed that patients with increased NLR prior to treatment exhibit reduced OS (HR = 1.58, 95% CI: 1.44-1.74; p &lt; 0.00001) and decreased PFS (HR = 1.48, 95% CI: 1.34-1.63; p &lt; 0.00001). Furthermore, elevated NLR significantly impacted disease-free survival (HR: 1.79, 95% CI: 1.18-2.71; p = 0.006), recurrence rates (HR: 2.18, 95% CI: 1.36-3.51; p = 0.001), recurrence-free survival (HR: 3.05, 95% CI: 1.79-5.19; p &lt; 0.0001), and the incidence of distant metastases (HR: 1.73, 95% CI: 1.20-2.50; p = 0.003).ConclusionAn elevated NLR prior to treatment demonstrates a strong association with decreased OS and PFS among patients with cervical cancer, underscoring the significance of NLR as a prognostic marker within this population.Systematic review registrationhttps://www.crd.york.ac.uk/PROSPERO/display_record.php?RecordID=529817, identifier CRD42024529817.
Prognostic significance of p27 in colorectal cancer: a meta-analysis and bioinformatics analysis
Authors: Zou Jing , Wang Dong , Yin Gaoping , Lu Kexiang , Chang Kaibin , Li He 
Published: December 2024

BackgroundIn the past, numerous investigations have delved into the influence of p27 (p27kip) on the prognosis and clinicopathological characteristics of colorectal cancer (CRC), yielding conclusions that are not universally statistically significant, thus rendering the discourse rather contentious.MethodsWe collected available articles published before August 2024 and extracted data to analyze the association between the expression of p27 and the prognosis and clinicopathological features of CRC. In addition, we used Gene Expression Profiling Interactive Analysis (GEPIA), University of Alabama at Birmingham’s Cancer Data Analysis Portal (UALCAN), and the Human Protein Atlas (HPA) to validate our results.ResultsThrough an extensive examination of four prominent databases, a total of 21 original articles encompassing a cohort of 3,378 patients were identified. The findings indicated that a low expression of p27 could lead to shorter overall survival (OS) [hazard ratio (HR) = 0.44, 95% confidence interval (95%CI) = 0.31–0.61, Z = 4.89, p = 0.000] and disease-free survival (DFS) (HR = 0.40, 95%CI = 0.28–0.59, Z = 4.75, p = 0.000). In addition, a low expression of p27 predisposed tumors to the right colon [odds ratio (OR) = 0.61, 95%CI = 0.46–0.82, Z = 3.32, p = 0.001] and limited tumor differentiation (OR = 0.56, 95%CI = 0.41–0.77, Z = 3.62, p = 0.000), but had no effect on TNM staging (OR = 0.80, 95%CI = 0.52–1.22, Z = 1.05, p = 0.295), lymph node metastasis (OR = 0.90, 95%CI = 0.25–3.28, Z = 0.16, p = 0.876), and tumor size (OR = 0.94, 95%CI = 0.54–1.65, Z = 0.21, p = 0.835). The results from GEPIA and UALCAN showed that p27 had no effect on TNM staging, lymph node metastasis, DFS, and OS; moreover, there was no expression difference between tumor tissues and normal tissues. The findings from the HPA indicated that there was lower expression of p27 in tumor tissues compared with normal tissues.ConclusionAlthough inconsistent results were reached with the bioinformatics analysis from this meta-analysis, it was confirmed that a low expression of p27 can adversely affect the prognosis of patients with CRC and make a meaningful impact on a part of the clinicopathological features in the meta-analysis with abundant data. In the future, predicting the prognosis of patients with CRC and guiding treatment might emerge as a significant objective.
A supercritical oil extract of Schisandra chinensis seeds ameliorates Huntington’s disease-like symptoms and neuropathology: the potential role of anti-oxidant and anti-inflammatory effects
Authors: Jo Hyo-Sung , Lee Youn-Woo , Son So-Ri , Jang Dae Sik , Woo Kwon Tae , Ha Yujeong , Moon Sang-Kwan , Kim Min Soo , Cho Ik-Hyun 
Published: December 2024

BackgroundHuntington disease (HD), a neurodegenerative autosomal dominant disorder, is characterized by involuntary choreatic movements with cognitive and behavioral disturbances. Up to now, no therapeutic strategies are available to completely ameliorate the progression of HD. Schisandra chinensis has various pharmacologic effects such as antioxidant and anti-inflammatory activities. However, the neuroprotective value of seed oil of S. chinensis (SOSC) has not been elucidated yet. The purpose of this study was to determine neuroprotective effects of SOSC by supercritical fluid extraction against 3-nitropropionic acid (3-NPA)-induced HD-like symptoms and neuropathology in an experimental mouse model.MethodsSOSC (75, 150, and 300 mg/kg/day) was orally pre-administration once daily at 1 hour before 3-NPA intoxication.ResultsSOSC ameliorated movement dysfunction and lethality following 3-NPA intoxication in connection with reduction of lesion area, neurodegeneration/apoptosis, microglial migration/activation, and mRNA expression of pro-inflammatory cytokines/enzymes in the striatum. SOSC inhibited the activation of nuclear factor-kappa B (NF-κB) and mitogen-activated protein kinase (MAPKs) pathways but stimulated nuclear factor erythroid 2-related factor 2 (Nrf2) in the striatum after 3-NPA intoxication. Schizandrin, a main component of SOSC, reduced protein expression levels of Iba-1 and p-NF-κB in 3-NPA-induced BV2 cells (murine microglia cell line). BV2 cell’s conditioned medium inhibited cleaved caspase-3 in 3-NPA-induced SH-SY5Y cells (a neuroblastoma cell line).ConclusionSOSC might ameliorate movement dysfunction by inhibiting neuropathology through its anti-inflammatory and antioxidant activities in the striata of 3-NPA-intoxicated mice. These findings suggest that SOSC could serve as a promising therapeutic candidate for HD-like symptoms, providing a foundation for future treatment strategies targeting neuroinflammation and oxidative stress.
Zhili decoction ameliorates ulcerative colitis by modulating gut microbiota and related metabolite, and inhibiting the TLR4/NF-κB/NLRP3 pathway
Authors: Tan Tianying , Chen Qin , Chen Ping , Li Shuangshuang , Hu Wenting , Yang Tao , Jia Yingtian 
Published: December 2024

Ethnopharmacological ImportanceZhili decoction (ZLD) is a traditional Chinese medicine prescription for ulcerative colitis (UC). However, the mechanism by which ZLD exerts its therapeutic effects in the context of UC remains unclear.Aim of studyThe aim of this study was to investigate the effects of ZLD on the gut microbiota and related fecal metabolite levels using a mouse model of UC. In addition, we examined the underlying molecular mechanisms responsible for these effects.Materials and methodsThe major components of ZLD were detected by ultra-performance liquid chromatography-mass spectrometry (UPLC-MS). An integrated approach employing 16S rRNA and fecal metabolomics was employed to assess the potential impacts of ZLD on gut flora abundance and diversity, fecal metabolite levels, and various metabolic pathways. To further investigate the potential mechanisms of ZLD in treating UC, the expression of genes and proteins were examined by RT-qPCR, immunohistochemical staining and Western blotting.ResultsZLD markedly alleviated symptoms and inflammatory injury in mice with UC. DSS induced notable alterations in the gut microbiome, and ZLD enhanced gut microbial diversity in UC mice by augmenting the abundance of Bacteroidota, Christensenella, Lactobacillus, etc., while reducing the Firmicutes/Bacteroidota ratio. ZLD treatment significantly modified the metabolic profile of mice with UC. It significantly suppressed the arachidonic acid metabolic pathway and promoted the butyrate acid metabolic pathway. ZLD reduced inflammatory factors and inhibited TLR4/NF-κB/NLRP3 pathway expression. In addition, correlation analysis demonstrated a close relationship between gut microbes, fecal metabolites, and inflammatory factors.ConclusionZLD alleviated UC by regulating gut flora, modulating related metabolite levels, and inhibiting TLR4/NF-κB/NLRP3 pathway.
Unveiling the immunomodulator role of plasma oxidized lipids in SA-AKI progression: a CRRT perspective
Authors: Zhou Lu , Li Huirong , Guo Wenfeng , Feng Lan , Hu Jiangtao , Liu Jing , Wang Tingting , Liu Hongbao 
Published: December 2024

BackgroundPlasma oxidized lipids are intimately linked to immune regulation as bioactive mediators. However, it is not clear whether they are related to the progression of sepsis-associated acute kidney injury (SA-AKI) and the effect of continuous renal replacement therapy (CRRT). This study intends to explore the changes in certain oxidized lipid during CRRT treatment and their correlation with the immune microenvironment and prognosis by analyzing plasma oxidative lipidomics.MethodsA total of 48 SA-AKI patients undergoing CRRT for more than 72 h were enrolled in this prospective cohort study. Oxidative lipidomics was analyzed by ultra performance liquid chromatography coupled with tandem mass spectrometric (UPLC-MS/MS) detection at the beginning of CRRT (T0) and 72 h later (T72), respectively.ResultsCompared with survivors, plasma EETs, EpOMEs and EpDPEs in non-survivors were significantly down-regulated at T0, while PGFs, TXB and HEPEs were up-regulated. After 72 h of CRRT, DiHETEs were significantly up-regulated and PGFs were down-regulated in non-survivors, while HEPEs and EpOMEs were up-regulated and 6keto-PGF1α was down-regulated in survivors. KEGG annotation showed that the differential lipids of survivors before and after CRRT were mainly enriched and up-regulated in metabolic pathway.ConclusionThis study provided a comprehensive overview of plasma oxidized lipids in SA-AKI patients undergoing CRRT and further elucidated the lipids and pathways linked to patient severity and prognosis. Additionally, we unveiled the potential mechanisms by which CRRT improves the prognosis of SA-AKI patients by removing PGFs and TXs while simultaneously upregulating HEPE to ameliorate the immune microenvironment, as well as the potential significance of adjusting CRRT prescriptions based on plasma oxidized lipidomics.
Metabolomic and metagenomic analyses elucidate the role of intercropping in mitigating continuous cropping challenges in tobacco
Authors: Liu Ming , Xue Rujun , Jin Shuangzhen , Gu Kaiyuan , Zhao Jie , Guan Shuyue , Xie Xiaoyu , Su Jiaen , Wang Longchang 
Published: December 2024

IntroductionCrop rotation of tobacco with other crops could effectively break the negative impact of continuous tobacco cropping, but the mechanisms of intercropping system effects on tobacco, especially on the rhizosphere, are not clear.MethodsIn this study, we investigated the impact of intercropping system on the diversity and function of tobacco metabolites and microorganisms through metabolomic and metagenomic analyses of the tobacco rhizosphere microenvironment intercropped with maize and soybean.ResultsThe results showed that the contents of huperzine b, chlorobenzene, and P-chlorophenylalanine in tobacco rhizosphere soils differed significantly among soybean-tobacco and maize-tobacco intercropping system. Chlorobenzene and P-chlorophenylalanine had the highest relative abundance under the soybean-tobacco intercropping system, and huperzine b had the highest relative abundance in the maize-tobacco cropping system. At the phylum level, the three most dominant strains were the same across all treatments: Proteobacteria, Actinobacteria, and Acidobacteria, with only minor differences in their abundance, with the fourth most abundant strain in both the tobacco monoculture. KEGG enrichment analysis of the tobacco rhizosphere soil microbiome revealed that intercropping significantly increased the abundance of metabolites in the ABC transporters pathway and up-regulated the LivK, LivH, Livg, LivM, and LivF genes of the branched-chain amino acid pathwayDiscussionCollectively, our results indicate that the intercropping could enhance the activity of Livs to enhance the ABC transport pathway, and thus improve the transmembrane transport ability of tobacco roots, thus reducing the negative impact of continuous tobacco cropping. At the same time, the maize-tobacco intercropping could promote the production and transportation of phenolic acids, flavonoids, and other bioactive substances in the tobacco root system, which could enhance tobacco adaptation capacity to abiotic stress.
Effect of vapor pressure deficit on growth and yield of pearl millet germplasm originating from semi-arid, semi-humid and humid regions
Authors: Garba Hadizatou , Hamidou Falalou , Abdou Harou , Burridge James , Vadez Vincent 
Published: December 2024

IntroductionThe increase in vapor pressure deficit (VPD) is among the expected change in futur climate, and understanding its effect on crop growth is of much significance for breeeding programs. Three groups (G1,G2 and G3) of pearl millet germplasm, originating from regions with different rainfall intensities, were grown in the field during period of high and low VPDs. The groups G1,G2 and G3 were respectively from Guinean (rainfall above 1000 mm), Soudanian (rainfall between 600 mm and 900 mm), and Sahelian zones (rainfall between 600 and 300 mm) of Africa. The objective was to assess if there was any growth response difference among the germplasm groups.MethodFour trials were conducted, two in the dry season of 2019 (Ds19) and 2020 (Ds20) with avarage VPDs of 3.62 kPa and 2.92 kPa, respectively, and two in the rainy season of 2019 (Rs19) and 2020 (Rs20) with avaerage VPDs of 1.14 kPa and 0.61 kPa, respectively.ResultsIn order to avoid possible confounding effects of radiation on millet growth and yield, data were normalized by the quantity of light received during each season. After this normalization, leaf area and grain yield decreased in the highest-VPD seasons whereas tiller number decreased only in Ds19 (one high VPD season). The comparison of the three germplasm groups indicates that G3 the germplasm group from Sahelian regions showed greater tolerance to high VPD than G1 and G2.DiscussionGermplasm from the G3 group could be a good material for developing tolerant germplasm to future climate that is bound to have high VPD.
The additional value of self-reflection and feedback on therapy outcome: a pilot study
Authors: Kamp David , Blanker Cynthia , Hafkenscheid Anton , van Os Jim 
Published: December 2024

IntroductionOver the past few decades, psychotherapy research was dominated by testing the efficacy of “brand name” therapeutic techniques and models. Another line of research however, suggests that common factors, such as the therapeutic alliance and empathy, might play a greater role in effective therapy than specific therapeutic techniques and models. Routine process monitoring (RPM), focusing on common factors, has emerged as a promising approach to enhance therapy outcomes. This ongoing feedback loop aims to improve the therapeutic alliance and to address countertransference issues, potentially leading to better therapy outcomes.MethodsA total of 131 patients above the age of 18 were included into the sample. The design of the study consisted of three stages, in which two kinds of instruments to measure the therapeutic alliance and countertransference were administered, namely the Session Rating Scale (SRS) and a brief version of Impact Message Inventory-Circumplex (IMI-CS). The Outcome Rating Scale (ORS) was used as an outcome measure. Depending on the stage of the study, these three instruments were administered every time a patient had a psychotherapy session.ResultsPatients reported improved outcomes on the ORS in T2 compared to T1 (p = 0.011). Furthermore, a significant increase in the strength of the therapeutic relationship, as measured by the SRS, occurred between T2 and T3 (p = 0.031). A positive correlation was found between SRS scores and the “friendly” position on the IMI-CS (p = &lt;0.000), while a negative correlation was found between SRS ratings and the “submissive” position on the IMI-CS (p = 0.019).DiscussionPatients experienced improvements and might attribute part of their improvement to the relationship with their therapists, evaluating the therapeutic alliance more positively in the later stages of their treatment. Despite methodological challenges, such as varying treatment durations and pre-existing therapist-patient relationships, the findings highlight the value of patient engagement and therapist self-reflection. This study supports the utility of deliberate practice in psychotherapy, suggesting it can enhance therapeutic outcome and laying the groundwork for future research.
Inner engineering is associated with perceived improvements in relationship quality, interpersonal connections, and interpersonal compassion
Authors: Jafari Nashaw , Manvelian Atina , Mohamed Enas , Rayapuraju Akila , Orui Hibiki , Alkuhaimi Sultan , Reed Preeti Upadhyay , Subramaniam Balachundhar 
Published: December 2024

ObjectivesInterpersonal relationships are a cornerstone of wellbeing. Mindfulness-based practices have been shown to improve relationship quality and reduce perceived stress. Inner Engineering (IE) is a transformative program that includes meditative and yogic practices associated with improvements in mindfulness and wellbeing.MethodsThis cross-sectional observational study enrolled participants who were already registered for the Inner Engineering program to learn Shambhavi Mahamudra Kriya, a multi-component, 21-min meditation. Of the 356 participants who consented, 290 participants downloaded the mobile 3Cs app to participate. The enrolled participants were asked to complete self-reported electronic questionnaires at three timepoints: before the program, post-program, and 6 weeks after the program. The following measures were used: the Positive-Negative Relationship Quality (PN-RQ) scale, Interpersonal Mindfulness Scale (IMS), Perceived Stress Scale (PSS), Compassion Scale (CS), and Flourishing Measure. Linear mixed-effects models were used to analyze the survey data, with p-values of &lt; 0.05 considered statistically significant.ResultsFrom the baseline to the 6-week follow-up after the program, the participants reported experiencing more positive qualities (p &lt; 0.001, ηp2 = 0.12) and fewer negative qualities (p = 0.001, ηp2 = 0.08) in their relationships, increased interpersonal mindfulness (p &lt; 0.001, ηp2 = 0.30), decreased stress levels (p &lt; 0.001, ηp2 = 0.27), enhanced compassion for others (p &lt; 0.001, ηp2 = 0.17), and overall personal wellbeing (p &lt; 0.001, ηp2 = 0.25).ConclusionParticipation in the Inner Engineering yoga program appears to be associated with improvements in relationships, interpersonal mindfulness, compassion, stress, and overall wellbeing.Clinical trial registrationClinicalTrials.gov, identifier: NCT05528978.
Effect of SNS addiction on prosocial behavior: mediation effect of fear of missing out
Authors: Xu Manjing , Liu Donglin , Yan Jinzhe 
Published: December 2024

PurposeThis study examines the relationship between social networking sites addiction and pro-social behavior, considering the increasing importance of social networking sites in daily life. It explores the mediating role of Fear of Missing Out in this relationship and investigates the moderating role of basic psychological need satisfaction.Research design, data, and methodologyThis study employed a snowball sampling method to conduct an online survey among social network users in China. The proposed model was tested using regression analysis to interpret the results.ResultsFindings indicate a negative predictive effect of social networking sites addiction on prosocial behavior. Misplaced fear partially mediates this relationship. Basic psychological need satisfaction significantly moderates the mediating effect of Fear of Missing Out on the relationship between SNS addiction and prosocial behavior.Practical implicationsThis study provides strategies for effectively preventing social networking sites addiction in real-world settings and mitigating its negative impact on individuals’ physical and mental health. It suggests interventions at four levels—individual, school, society, and government—to enhance basic psychological need satisfaction, thereby improving prosocial behavior and facilitating the promotion of interpersonal interactions and the equitable, harmonious development of society.