bluuebunny/crossref_metadata_2025_split huggingface dataset:
Species versus within-species niches: a multi-modelling approach to assess range size of a spring-dwelling amphibian
Authors: Goudarzi Forough , Hemami Mahmoud-Reza , Malekian Mansoureh , Fakheran Sima , Martínez-Freiría Fernando 
Published: January 2021

AbstractSpecies Distribution Models (SDMs) can be used to estimate potential geographic ranges and derive indices to assess species conservation status. However, habitat-specialist species require fine-scale range estimates that reflect resource dependency. Furthermore, local adaptation of intraspecific lineages to distinct environmental conditions across ranges have frequently been neglected in SDMs. Here, we propose a multi-stage SDM approach to estimate the distributional range and potential area of occupancy (pAOO) ofNeurergus kaiseri,a spring-dwelling amphibian with two climatically-divergent evolutionary lineages. We integrate both broad-scale climatic variables and fine-resolution environmental data to predict the species distribution while examining the performance of lineage-level versus species-level modelling on the estimated pAOO. Predictions of habitat suitability at the landscape scale differed considerably between evolutionary level models. At the landscape scale, spatial predictions derived from lineage-level models showed low overlap and recognised a larger amount of suitable habitats than species-level model. The variable dependency of lineages was different at the landscape scale, but similar at the local scale. Our results highlight the importance of considering fine-scale resolution approaches, as well as intraspecific genetic structure of taxa to estimate pAOO. The flexible procedure presented here can be used as a guideline for estimating pAOO of other similar species.
Downregulation of CR6-interacting factor 1 suppresses keloid fibroblast growth via the TGF-β/Smad signaling pathway
Authors: Nagar Harsha , Kim Sungmin , Lee Ikjun , Kim Seonhee , Choi Su-Jeong , Piao Shuyu , Jeon Byeong Hwa , Oh Sang-Ha , Kim Cuk-Seong 
Published: January 2021

AbstractKeloids are a type of aberrant skin scarring characterized by excessive accumulation of collagen and extracellular matrix (ECM), arising from uncontrolled wound healing responses. While typically non-pathogenic, keloids are occasionally regarded as a form of benign tumor. CR6-interacting factor 1 (CRIF1) is a well-known CR6/GADD45-interacting protein, that has both nuclear and mitochondrial functions, and also exerts regulatory effects on cell growth and apoptosis. In this study, cell proliferation, cell migration, collagen production and TGF-β signaling was compared between normal fibroblasts (NFs) and keloid fibroblasts (KFs). Subsequently, the effects of CRIF1 deficiency were investigated in both NFs and KFs. Cell proliferation, cell migration, collagen production and protein expressions of TGF-β, phosphorylation of Smad2 and Smad3 were all found to be higher in KFs compared to NFs. CRIF1 deficiency in NFs and KFs inhibited cell proliferation, migration, and collagen production. In addition, phosphorylation of Smad2 and Smad3, which are transcription factors of collagen, was decreased. In contrast, mRNA expression levels of Smad7 and SMURF2, two important inhibitory proteins of Smad2/3, were increased, suggesting that CRIF1 may regulate collagen production. CRIF1 deficiency decreases the proliferation and migration of KFs, thereby inhibiting their overgrowth via the transforming growth factor-β (TGF-β)/Smad pathway. CRIF1 may therefore represent a potential therapeutic target in keloid pathogenesis.
Intentional synchronisation affects automatic imitation and source memory
Authors: Cross Liam , Atherton Gray , Sebanz Natalie 
Published: January 2021

AbstractActing in synchrony is a fundamental part of many social interactions and can have pro-social consequences. Explanations for this relationship were investigated here using implicit measures of imitation (automatic imitation task) and memory (preference overlap task). In Study 1, participants performed an intentional synchronisation task where they moved sliders in or out of time with another person while a third person observed. Those who had moved in synchrony showed a stronger tendency to imitate their partner’s actions than those who had moved in a non-synchronous way. Similarly, coordinated partners were also more likely to share object preferences. Results also showed that rather than memory blurring between co-actors, participants had improved memories for the self. Study 2 exchanged intentional for incidental coordination (coordinating with a synchronous metronome). None of the findings from Study 1 replicated when synchronisation was incidental rather than intentional, suggesting that having a shared goal may be critical for triggering effects of synchronisation on imitation tendencies and memory. Together these findings favour explanations related to changes in social categorisation over representational overlap between co-actors.
Influence of different elicitors on BIA production in Macleaya cordata
Authors: Huang Peng , Xia Liqiong , Zhou Li , Liu Wei , Wang Peng , Qing Zhixing , Zeng Jianguo 
Published: January 2021

AbstractSanguinarine (SAN) and chelerythrine (CHE) have been widely used as substitutes for antibiotics for decades. For a long time, SAN and CHE have been extracted from mainlyMacleaya cordata, a plant species that is a traditional herb in China and belongs to the Papaveraceae family. However, with the sharp increase in demand for SAN and CHE, it is necessary to develop a new method to enhance the supply of raw materials. Here, we used methyl jasmonate (MJ), salicylic acid (SA) and wounding alone and in combination to stimulate aseptic seedlings ofM. cordataat 0 h, 24 h, 72 h and 120 h and then compared the differences in metabolic profiles and gene expression. Ultimately, we found that the effect of using MJ alone was the best treatment, with the contents of SAN and CHE increasing by 10- and 14-fold, respectively. However, the increased SAN and CHE contents in response to combined wounding and MJ were less than those for induced by the treatment with MJ alone. Additionally, after MJ treatment, SAN and CHE biosynthetic pathway genes, such as those encoding the protopine 6-hydroxylase and dihydrobenzophenanthridine oxidase enzymes, were highly expressed, which is consistent with the accumulation of SAN and CHE. At the same time, we have also studied the changes in the content of synthetic intermediates of SAN and CHE after elicitor induction. This study is the first systematic research report about using elicitors to increase the SAN and CHE inMacleaya cordata.
Direct measurement of Coulomb-laser coupling
Authors: Azoury Doron , Krüger Michael , Bruner Barry D. , Smirnova Olga , Dudovich Nirit 
Published: January 2021

AbstractThe Coulomb interaction between a photoelectron and its parent ion plays an important role in a large range of light-matter interactions. In this paper we obtain a direct insight into the Coulomb interaction and resolve, for the first time, the phase accumulated by the laser-driven electron as it interacts with the Coulomb potential. Applying extreme-ultraviolet interferometry enables us to resolve this phase with attosecond precision over a large energy range. Our findings identify a strong laser-Coulomb coupling, going beyond the standard recollision picture within the strong-field framework. Transformation of the results to the time domain reveals Coulomb-induced delays of the electrons along their trajectories, which vary by tens of attoseconds with the laser field intensity.
Development and validation of deep learning algorithms for automated eye laterality detection with anterior segment photography
Authors: Zheng Ce , Xie Xiaolin , Wang Zhilei , Li Wen , Chen Jili , Qiao Tong , Qian Zhuyun , Liu Hui , Liang Jianheng , Chen Xu 
Published: January 2021

AbstractThis paper aimed to develop and validate a deep learning (DL) model for automated detection of the laterality of the eye on anterior segment photographs. Anterior segment photographs for training a DL model were collected with the Scheimpflug anterior segment analyzer. We applied transfer learning and fine-tuning of pre-trained deep convolutional neural networks (InceptionV3, VGG16, MobileNetV2) to develop DL models for determining the eye laterality. Testing datasets, from Scheimpflug and slit-lamp digital camera photography, were employed to test the DL model, and the results were compared with a classification performed by human experts. The performance of the DL model was evaluated by accuracy, sensitivity, specificity, operating characteristic curves, and corresponding area under the curve values. A total of 14,468 photographs were collected for the development of DL models. After training for 100 epochs, the DL models of the InceptionV3 mode achieved the area under the receiver operating characteristic curve of 0.998 (with 95% CI 0.924–0.958) for detecting eye laterality. In the external testing dataset (76 primary gaze photographs taken by a digital camera), the DL model achieves an accuracy of 96.1% (95% CI 91.7%–100%), which is better than an accuracy of 72.3% (95% CI 62.2%–82.4%), 82.8% (95% CI 78.7%–86.9%) and 86.8% (95% CI 82.5%–91.1%) achieved by human graders. Our study demonstrated that this high-performing DL model can be used for automated labeling for the laterality of eyes. Our DL model is useful for managing a large volume of the anterior segment images with a slit-lamp camera in the clinical setting.
Real-world evidence and optimization of vocal dysfunction in end-stage renal disease patients with secondary hyperparathyroidism
Authors: Chang Geng-He , Chou Fong-Fu , Tsai Ming-Shao , Tsai Yao-Te , Yang Ming-Yu , Huang Ethan I. , Su Hui-Chen , Hsu Cheng-Ming 
Published: January 2021

AbstractPatients with end-stage renal disease (ESRD) may demonstrate secondary hyperparathyroidism (SHPT), characterized by parathyroid hormone oversecretion in response to electrolyte imbalance (e.g., hypocalcemia and hyperphosphatemia). Moreover, this electrolyte imbalance may affect vocal cord muscle contraction and lead to voice change. Here, we explored the effects of SHPT on the voices of patients with ESRD. We used data of 147,026 patients with ESRD from the registry for catastrophic illness patients, a sub-database of Taiwan National Health Insurance Research Database. We divided these patients into 2 groups based on whether they had hyperparathyroidism (HPT) and compared vocal dysfunction (VD) incidence among them. We also prospectively included 60 ESRD patients with SHPT; 45 of them underwent parathyroidectomy. Preoperatively and postoperatively, voice analysis was used to investigate changes in vocal parameters. In the real-world database analysis, the presence of HPT significantly increased VD incidence in patients with ESRD (p = 0.003): Cox regression analysis results indicated that patients with ESRD had an approximately 1.6-fold increased VD risk (p = 0.003). In the clinical analysis, the “jitter” and “shimmer” factors improved significantly after operation, whereas the aerodynamic factors remained unchanged. In conclusion, SHPT was an independent risk factor for VD in patients with ESRD, mainly affecting their acoustic factors.
Wear of hip prostheses increases serum IGFBP-1 levels in patients with aseptic loosening
Authors: Vallés Gema , García-Rey Eduardo , Saldaña Laura , García-Cimbrelo Eduardo , Vilaboa Nuria 
Published: January 2021

AbstractThe biological mechanisms involved in aseptic loosening include inflammation-associated and bone resorption-associated processes. Coordinated cellular actions result in biochemical imbalances with devastating consequences for the joint. Given that this condition is not known for showing systemic signs, we investigated whether circulating levels of inflammation-related proteins are altered in patients with aseptic loosening. Our study included 37 patients who underwent revision surgery due to hip osteolysis and aseptic loosening and 31 patients who underwent primary total hip arthroplasty. Using antibody arrays, we evaluated the serum levels of 320 proteins in four patients from each group. The results showed differences in insulin-like growth factor-binding protein 1 (IGFBP-1) concentrations, which we then quantified using enzyme-linked immunosorbent assay tests in all study patients. The results confirmed that serum IGFBP-1 concentrations were higher in the revision surgery patients than in the hip arthroplasty patients. In vitro studies showed that exposure of human osteoblasts to titanium particles induced an IGFBP-1 release that further increased when exposure to particles was performed in media conditioned by human M1 macrophages. These findings suggest that elevated serum IGFBP-1 levels in patients with aseptic loosening can arise from increased local IGFBP-1 production in the inflammatory environment of the periprosthetic bed.
Effects of a bacteria-produced algicide on non-target marine invertebrate species
Authors: Simons Victoria E. , Coyne Kathryn J. , Warner Mark E. , Dolan Margaret M. , Cohen Jonathan H. 
Published: January 2021

AbstractHarmful algal blooms (HABs) affect both freshwater and marine systems. Laboratory experiments suggest an exudate produced by the bacteriumShewanellasp. IRI-160 could be used to prevent or mitigate dinoflagellate blooms; however, effects on non-target organisms are unknown. The algicide (IRI-160AA) was tested on various ontogenetic stages of the copepodAcartia tonsa(nauplii and adult copepodites), the blue crabCallinectes sapidus(zoea larvae and megalopa postlarvae), and the eastern oysterCrassostrea virginica(pediveliger larvae and adults). Mortality experiments withA. tonsarevealed that the 24-h LC50 was 13.4% v/v algicide for adult females and 5.96% for early-stage nauplii. ForC. sapidus, the 24-h LC50 for first-stage zoeae was 16.8%; results were not significant for megalopae or oysters. Respiration rates for copepod nauplii increased in the 11% concentration, and in the 11% and 17% concentrations for crab zoeae; rates of later stages and oysters were unaffected. Activity level was affected for crab zoeae in the 1%, 11%, and 17% treatments, and for oyster pediveliger larvae at the 17% level. Activity of later stages and of adult copepods was unaffected. Smaller, non-target biota with higher surface to volume could be negatively impacted from IRI-160AA dosing, but overall the taxa and stages assayed were tolerant to the algicide at concentrations required for dinoflagellate mortality (EC50 =  ~ 1%).
Decreased resting-state alpha-band activation and functional connectivity after sleep deprivation
Authors: Wu Jintao , Zhou Qianxiang , Li Jiaxuan , Chen Yang , Shao Shuyu , Xiao Yi 
Published: January 2021

AbstractCognitive abilities are impaired by sleep deprivation and can be recovered when sufficient sleep is obtained. Changes in alpha-band oscillations are considered to be closely related to sleep deprivation. In this study, power spectrum, source localization and functional connectivity analyses were used to investigate the changes in resting-state alpha-band activity after normal sleep, sleep deprivation and recovery sleep. The results showed that the global alpha power spectrum decreased and source activation was notably reduced in the precuneus, posterior cingulate cortex, cingulate gyrus, and paracentral lobule after sleep deprivation. Functional connectivity analysis after sleep deprivation showed a weakened functional connectivity pattern in a widespread network with the precuneus and posterior cingulate cortex as the key nodes. Furthermore, the changes caused by sleep deprivation were reversed to a certain extent but not significantly after one night of sleep recovery, which may be due to inadequate time for recovery sleep. In conclusion, large-scale resting-state alpha-band activation and functional connectivity were weakened after sleep deprivation, and the inhibition of default mode network function with the precuneus and posterior cingulate cortex as the pivotal nodes may be an important cause of cognitive impairment. These findings provide new insight into the physiological response to sleep deprivation and determine how sleep deprivation disrupts brain alpha-band oscillations.
HLA genotype-clinical phenotype correlations in multiple sclerosis and neuromyelitis optica spectrum disorders based on Japan MS/NMOSD Biobank data
Authors: Watanabe Mitsuru , Nakamura Yuri , Sato Shinya , Niino Masaaki , Fukaura Hikoaki , Tanaka Masami , Ochi Hirofumi , Kanda Takashi , Takeshita Yukio , Yokota Takanori , Nishida Yoichiro , Matsui Makoto , Nagayama Shigemi , Kusunoki Susumu , Miyamoto Katsuichi , Mizuno Masanori , Kawachi Izumi , Saji Etsuji , Ohashi Takashi , Shimohama Shun , Hisahara Shin , Nishiyama Kazutoshi , Iizuka Takahiro , Nakatsuji Yuji , Okuno Tatsusada , Ochi Kazuhide , Suzumura Akio , Yamamoto Ken , Kawano Yuji , Tsuji Shoji , Hirata Makoto , Sakate Ryuichi , Kimura Tomonori , Shimizu Yuko , Nagaishi Akiko , Okada Kazumasa , Hayashi Fumie , Sakoda Ayako , Masaki Katsuhisa , Shinoda Koji , Isobe Noriko , Matsushita Takuya , Kira Jun-ichi 
Published: January 2021

AbstractHLA genotype-clinical phenotype correlations are not established for multiple sclerosis (MS) and neuromyelitis optica spectrum disorders (NMOSD). We studied HLA-DRB1/DPB1 genotype–phenotype correlations in 528 MS and 165 NMOSD cases using Japan MS/NMOSD Biobank materials. HLA-DRB1*04:05, DRB1*15:01 and DPB1*03:01 correlated with MS susceptibility and DRB1*01:01, DRB1*09:01, DRB1*13:02 and DPB1*04:01 were protective against MS. HLA-DRB1*15:01 was associated with increased optic neuritis and cerebellar involvement and worsened visual and pyramidal functional scale (FS) scores, resulting in higher progression index values. HLA-DRB1*04:05 was associated with younger onset age, high visual FS scores, and a high tendency to develop optic neuritis. HLA-DPB1*03:01 increased brainstem and cerebellar FS scores. By contrast, HLA-DRB1*01:01 decreased spinal cord involvement and sensory FS scores, HLA-DRB1*09:01 decreased annualized relapse rate, brainstem involvement and bowel and bladder FS scores, and HLA-DRB1*13:02 decreased spinal cord and brainstem involvement. In NMOSD, HLA-DRB1*08:02 and DPB1*05:01 were associated with susceptibility and DRB1*09:01 was protective. Multivariable analysis revealed old onset age, long disease duration, and many relapses as independent disability risks in both MS and NMOSD, and HLA-DRB1*15:01 as an independent risk only in MS. Therefore, both susceptibility and protective alleles can influence the clinical manifestations in MS, while such genotype–phenotype correlations are unclear in NMOSD.
Modelling seasonal patterns of larval fish parasitism in two northern nearshore areas in the Humboldt Current System
Authors: Paredes Lissette D. , Landaeta Mauricio F. , Molinet Carlos , González M. Teresa 
Published: January 2021

AbstractMacro- and micro-environmental factors modulate parasite loads in fish, determining parasitic abundances, diversity, and interaction dynamics. In this study, seasonal variations in larval ectoparasites on fish larvae in the northern Humboldt Current System (HCS) were evaluated using a delta-gamma generalized linear model to predict their occurrence frequencies. Fish larvae were collected from two nearshore areas during austral spring–summer and autumn–winter. Only five (of 38) larval fish species were parasitized by copepods: Gobiesox marmoratus, Ophiogobius jenynsi, Helcogrammoides cunninghami, Myxodes sp., and Auchenionchus crinitus. A binomial model showed that the presence/absence of parasitized fish larvae varied among the fish species and their larval abundances, while a positive delta-gamma model showed that ectoparasite frequency varied among the seasons and fish species. Seasonal variations in parasitized fish larvae frequency could be associated with host and parasite reproductive processes, which are related to oceanographic features responsible for larval retention and subsequent higher infestation probabilities. Host length was positively correlated with ectoparasite length, suggesting early infection and combined growth until the detachment of the ectoparasite. Our results suggest that infestation patterns in larval fish species can be identified using delta-gamma models and that they respond to local (retention) and high-scale (HCS) processes.
Aortic acceleration as a noninvasive index of left ventricular contractility in the mouse
Authors: Perez Jorge Enrique Tovar , Ortiz-Urbina Jesus , Heredia Celia Pena , Pham Thuy T. , Madala Sridhar , Hartley Craig J. , Entman Mark L. , Taffet George E. , Reddy Anilkumar K. 
Published: January 2021

AbstractThe maximum value of the first derivative of the invasively measured left ventricular (LV) pressure (+ dP/dtmax or P′) is often used to quantify LV contractility, which in mice is limited to a single terminal study. Thus, determination of P′ in mouse longitudinal/serial studies requires a group of mice at each desired time point resulting in “pseudo” serial measurements. Alternatively, a noninvasive surrogate for P′ will allow for repeated measurements on the same group of mice, thereby minimizing physiological variability and requiring fewer animals. In this study we evaluated aortic acceleration and other parameters of aortic flow velocity as noninvasive indices of LV contractility in mice. We simultaneously measured LV pressure invasively with an intravascular pressure catheter and aortic flow velocity noninvasively with a pulsed Doppler probe in mice, at baseline and after the administration of the positive inotrope, dobutamine. Regression analysis of P′ versus peak aortic velocity (vp), peak velocity squared/rise time (vp2/T), peak (+ dvp/dt or v′p) and mean (+ dvm/dt or v′m) aortic acceleration showed a high degree of association (P′ versus: vp, r2 = 0.77; vp2/T, r2 = 0.86; v′p, r2 = 0.80; and v′m, r2 = 0.89). The results suggest that mean or peak aortic acceleration or the other parameters may be used as a noninvasive index of LV contractility.
CTCF and EGR1 suppress breast cancer cell migration through transcriptional control of Nm23-H1
Authors: Wong Ka Ming , Song Jiaxing , Wong Yung H. 
Published: January 2021

AbstractTumor metastasis remains an obstacle in cancer treatment and is responsible for most cancer-related deaths. Nm23-H1 is one of the first metastasis suppressor proteins discovered with the ability to inhibit metastasis of many cancers including breast, colon, and liver cancer. Although loss of Nm23-H1 is observed in aggressive cancers and correlated with metastatic potential, little is known regarding the mechanisms that regulate its cellular level. Here, we examined the mechanisms that control Nm23-H1 expression in breast cancer cells. Initial studies in aggressive MDA-MB-231 cells (expressing low Nm23-H1) and less invasive MCF-7 cells (expressing high Nm23-H1) revealed that mRNA levels correlated with protein expression, suggesting that transcriptional mechanisms may control Nm23-H1 expression. Truncational analysis of the Nm23-H1 promoter revealed a proximal and minimal promoter that harbor putative binding sites for transcription factors including CTCF and EGR1. CTCF and EGR1 induced Nm23-H1 expression and reduced cell migration of MDA-MB-231 cells. Moreover, CTCF and EGR1 were recruited to the Nm23-H1 promoter in MCF-7 cells and their expression correlated with Nm23-H1 levels. This study indicates that loss of Nm23-H1 in aggressive breast cancer is apparently caused by downregulation of CTCF and EGR1, which potentially drive Nm23-H1 expression to promote a less invasive phenotype.