bluuebunny/crossref_metadata_2025_split huggingface dataset:
Analytical Quality by Design–Based Approach for the Assay Method Development of Nusinersen Using Liquid Chromatography–High‐Resolution Mass Spectrometry
Authors: Bhalekar Vijay S. , Shah Ravi P. 
Published: December 2024

ABSTRACTThe quality assessment of oligonucleotide possesses challenges due to non‐distinguishable structurally similar impurities. These impurities are difficult to separate from the drug, and the assay method as per regulatory requirements must quantify only the drug to assign the potency of the oligonucleotide therapeutics. In the present study, we have proposed a hyphenated liquid chromatography (LC)–high resolution mass spectrometry (HRMS) approach for accurate quantification of oligonucleotides with an example of Nusinersen. An analytical quality by design (AQbD) approach has been proposed for “fit‐for‐purpose” LC method development through the Taguchi orthogonal array (OA) screening model and Box–Behnken optimization model. The developed LC method was capable of providing reasonable separation, where the specificity of the assay method was achieved through high resolution MS. The extracted ion chromatogram (EIC) with an accuracy range of 10 ppm from MS output has provided a reasonable symmetric peak for quantification. The method was also successfully employed for the Nusinersen formulation. The method validation was performed according to ICH Q2(R2) regulatory guidelines. A developed method through EIC processing was found to be specific and having linear response for 1.6–3 µg/mL. The percentage relative standard deviation was found to be less than 5 for repeatability and intermediate precision. The standard addition approach was used to determine assay method accuracy, which was found to be well within 97%–103% for triplicate at three concentrations. The current method was found to be robust with respect to LC flow rate, LC injection volume, MS drying/desolvation temperature, MS gas flow, and mass accuracy. The AQbD‐based quick LC separation coupled with HRMS methodology has been identified as a promising solution to assess the quality of oligonucleotide therapeutics.
Building Partnership Through Malaria Research in Thailand
Authors: Spring Michele , Jongsakul Krisada 
Published: December 2024

AbstractThe Armed Forces Research Institute of Medical Sciences (AFRIMS) is a US Department of Defense (DoD) institution under the US Embassy in Bangkok, Thailand. In partnership with the Royal Thai Army, AFRIMS has conducted in-depth malaria research for the past 60 years, with a specific focus on anti-malarial product development as well as monitoring and surveillance of product effectiveness and signs of anti-malarial drug resistance. While the primary mission is DoD-sponsored medical research to protect armed services personnel, developments and accomplishments are shared with the community at large. Conducting malaria research, particularly human clinical studies, requires investments in time, resources, and people, as well as dedicated partnerships. This commitment to medical research by its very nature does not provide rapid responses to epidemics or outbreaks. However, through its long-term collaborations and relationships, AFRIMS has been asked to assist with outbreaks in infectious diseases such as malaria, diarrhea, avian influenza and Zika. As a research lab, AFRIMS cannot readily provide diagnostic testing or clinical care, requiring regulatory and ethical approvals to engage in such. The time it takes to meet all logistical requirements may exceed the time interval for which interventions are urgently needed. In this chapter, the AFRIMS response to a malaria outbreak in Thailand is described, outlining the strengths and hurdles encountered. While steps can be taken to facilitate AFRIMS’ involvement in epidemic responses, ultimately it will need to be better incorporated, and planned for, within the primary “inter-epidemic” research mission.
Gender Equity in Academia Thriving as a Clinician-Scientist, Establishing Partnerships, and Driving Policy for Change in the Kenya Context
Authors: Mutebi Miriam , Kitulu Jacqueline , Ngaruiya Christine 
Published: December 2024

AbstractIn global health, the mantra that men lead while women do the work is one that is not lost on us. As women in global health, who have also been part of many global health partnerships, we are keen to challenge the status quo on how we think about impact of global health—a viewpoint that must account for gender equity at every level of partnership and global health initiatives. Our stories provide a window into the experiences of what that status quo currently looks like, with personal reflections on how that has impacted us and our own potential impact—by and large negatively so. We also describe potential solutions based on these experiences supplemented by literature on this topic; these are samples of the problem, albeit not comprehensive. To be sure, the challenges go beyond our own in breadth and depth. They will need continued purposeful and intentional work at the core of addressing the educational pipeline, advancing female academics equitably to their male counterparts, promotion and sponsoring for leadership positions equitably among others. This mindset and paradigm shift needs to start at the level of developing global health experts, with the students, trainees, and learners who will soon take over in our stead—it needs to start with you.
When Women Lead in Global Health: Alternative Mobilizations
Authors: Alonso Cristina , Torres Irene , Profeta Barbara 
Published: December 2024

AbstractOngoing discussions on what is “wrong” with global health or how to decolonialize global health tend to focus exclusively on structural shortcomings, such as effectiveness of global platforms and institutions or lack of truly participatory consultation strategies (downstream perspective). Thereby they fail to capture alternative approaches to global health leadership (upstream perspective) and to recognize the sovereignty of non-Western knowledge and the intrinsic value of community regeneration in all its forms as a key ingredient for effective global health practice. Women’s perspectives have been largely relegated to the gender agenda (balance, parity, equity) or proposed as models on “how to lead better” based on preconceived, male-based notions of what constitutes “effective” leadership, including setting and prioritizing goals. Instead of creating hierarchies of priorities, we must understand the complexity of human experience and the power and inequality patterns it is embedded in, and remain open to or embrace apparent contradictions when designing support mechanisms. This chapter centers on the disproportionate impact of COVID-19 in the lives of Latin American immigrants in Chelsea, Massachusetts, United States, during the 2020 pandemic. The chapter explores how public health prioritization of basic needs neither captured the relationship between impact and social response nor acknowledged the interplay between the different needs of people. Employing an insider’s look, we describe how the women leaders of the local organization La Colaborativa called upon culturally accepted codes to reverse power roles, questioned scientific definitions of needs and led their community out of potentially irreversible consequences of the crisis in a holistic and sustainable way. Their leadership serves as an example of how self-governed, women-led organizations that are rooted in the community may address the real needs of its members during a global catastrophic event.
Engaging with Heart in Global Health Partnerships
Authors: Luzadis Valerie A. 
Published: December 2024

AbstractWhat does it mean to build a more peaceful, equitable and loving world, to solve problems together? Global health partnerships are a perfect place to explore ways to do this because they rely on relationships. However, we are coming out of a dominant paradigm of Western science and colonialism that is often an invisible driver of our thoughts and actions. This frequently invisible yet insidious history still frames much of our understanding and our actions in every facet of life, including in science and global health. Continuing business-as-usual, without deep introspection and intention, leads us to replicate inequities and instabilities within our relationships with each other and between people and nature. These are experienced as traumas by all of us, some more easily seen than others. Many appear as individual and widespread human health concerns, as well as social and environmental disruption and degradation. This chapter explores ways in which we can create opportunities for healing these traumas while working together on solutions to immediate and long-term global health issues.
Risk Identification of Large-Scale Landslides Triggered by Rainfalls and Post-Rainfall Earthquakes in Sri Lanka
Authors: Sassa Kyoji , Doan Loi , Matsunami Koji , Miyagi Toyohiko , Weerasnghe Ranjan , Munasinghe Tania , Thaldena Nilmini , Karunawardena Asiri , Konagai Kazuo 
Published: December 2024

AbstractBased on the ISDR-ICL Sendai Landslide Partnerships 2015–2025, the ICL and NBRO implemented joint research on the “Development of early warning technology of rain-induced rapid and long-travelling landslides in Sri Lanka” from 2019. This paper reports the achievement of this project from June 2019 to April 2024.Based on studies of previous large-scale landslides, most large-scale landslides occurred in/around previous large-scale landslides. Then, we identified around 200 previous large-scale landslides in the Aranayake area (20 km × 20 km). Within 50 landslides, we conducted the LS-RAPID analysis, measured the critical pore pressure ratio and identified and recorded the resulted landslide moving area (landslide hazard map). Using MSSG (Multi-Scale Simulator for the Geoenvironment), we obtained the spatial rainfall data and calculated the pore water pressure for each landslide. When the pore water pressure in a potential landslide site exceeds the critical pore water pressure, a landslide moving area (hazard map) is identified. The rainfall and landslide hazard areas are forecasted and presented on an AR (Augmented reality) view for early warning.The latter part of this paper was devoted to earthquake and microearthquake monitoring in Sri Lanka using the developed ICL type seismographs, which include one velocity type of seismometer and one acceleration type seismometer in one unit. We installed this type of 15 seismographs in Sri Lanka in 2023–2024.Many nearby earthquakes (P-S time less than 5 s) were monitored throughout the year. The monitoring results are surprising. Nearby earthquakes are small in the magnitude. However, the distance between the landslide and its hypocenters are close. Therefore, the acceleration loading on the landslide is not small. Shaking by the nearby earthquakes in Aranayake and Atwelthota landslides can be the order of 0.01 kine (cm/s), which corresponds to the order of 1 gal (cm/s2) acceleration. The shaking by the Hambantota earthquake (Mg = 4.4) was 2–5 gal at the Aranayake landslide. The effects of nearby earthquakes after the rainfall were examined in a test slope near the Aranayake.In February 2024, two seismographs were installed in the Kotmale dam reservoir slope, where a series of past rockslide topography can be identified. Subsequently, many nearby earthquakes were monitored in March and April in 2024. This paper presents and discusses two seismic record examples (P-S time = 3.4 s on 2 March 2024 and P-S time = 0.1 s on 1 April 2024). Distance to the seismic source were 25–30 km and less than 1 km and maximum accelerations were 0.8 and 0.6 gal.
Viral Poetics as Performative Philosophy of Language
Authors: Mills Philip 
Published: December 2024

Abstract
In Chap. 3, we operated a shift from the parasite to the virus metaphor that overturns Austin’s evaluation of poetic utterances and gives them a space within OLP. This shift shows how poetry can provide insights towards a performative philosophy of language and exchange a conservative picture of language for a creative and inventive one. In one of her poems, Eve Kosofsky Sedgwick says: ‘Always, I wanted marriage inside out’ (Sedgwick 1993, 34). Insofar as marriage is exemplary of the performativity of language for Austin, I want the performative inside out. To want the performative inside out means to uncover an alternative that lies dormant behind the normative performative that excludes, silences, oppresses those who do not conform, to activate a virus to create an alternative space. We have seen how connecting the poetic to perlocution offers an alternative to Austin’s conception of language, and it remains to be seen how this alternative affects literary theory.
Poetic Documents: Transforming Forms of Language, Transforming Forms of Life
Authors: Mills Philip 
Published: December 2024

Abstract
This chapter focuses on a special kind of poetic dispositif that transforms forms of language and forms of life, namely poetic documents. A poetic document follows the move that I have described from the poem as a fixed object (and of poetry as a genre with well-defined properties) to a performative conception of a poetic dispositif (operating between word and world, author and reader, etc.). It operates by rearranging pre-existing texts and thus breaks down the traditional distinction between the poetic and the ordinary. One of the reasons that might explain this turn to document in contemporary French poetry is the multiplication of information. In The Poetics of Information Overload, Paul Stephens shows how poetry evolved with and adapted to new technologies throughout the twentieth century. He argues that ‘the poetics of information overload show that there are many possible forms, as well as frames of reference, available to contemporary poetry’ (Stephens 2015, 36). Poetic documents are one form of adaptation to technological changes and the transformative process at play in these poetic practices in turn affects the readers who can change their perception of the world, who can now see aspects that were hidden by the normativity of language and perception. Poetic documents reveal a transformative force of poetry that affects the uses of language and ways of being in the world, forms of language and forms of life. I will show in this chapter that even a minimal intervention in the original text can lead to radical change, understood more specifically through the philosophical notions of linguistic oppression and epistemic injustice.
Prototypen von Hürden im Beweisprozess
Authors: Abels Nele 
Published: December 2024

ZusammenfassungIm Beweisprozess gibt es viele Schwierigkeiten. Es ist nicht einfach, einen Beweis zu finden, gute von nicht so guten Beweisideen zu unterscheiden und bis zum Ende durchzuhalten. In diesem Kapitel wird daher die metakognitive Aktivität des Monitorings untersucht und ihre Interaktion mit Fachwissen in sechs verschiedenen Prototypen von Hürden im Beweisprozess verdeutlicht. Dabei wird auch das Niveau von Monitoringaktivitäten miteinbezogen.
Argumentieren und Beweisen
Authors: Abels Nele 
Published: December 2024

ZusammenfassungIn diesem Kapitel werden die Begriffe Argumentieren und Beweisen aus unterschiedlichen Blickwinkeln betrachtet, miteinander in Beziehung gebracht und der Ablauf des Argumentierens und Beweisens beschrieben. Darauf aufbauend wird das Argumentations- und Beweisverständnis für die vorliegende Arbeit herausgearbeitet. Des Weiteren wird auf die Rekonstruktion von Argumentationen eingegangen und der Forschungsstand zum Argumentieren und Beweisen in Bezug auf Schülerinnen und Schüler sowie (angehende) Lehrkräfte dargestellt.
Zum Kontext Geometrie
Authors: Abels Nele 
Published: December 2024

ZusammenfassungDieses Kapitel beinhaltet eine kurze Geschichte der Geometrie und des Geometrieunterrichts und fokussiert das geometrische Beweisen, die Bedeutung von Veranschaulichungen und den Einfluss von dynamischer Geometriesoftware.
Aphra Behns Oroonoko. Or the Royal Slave. A true History (1688) und die deutsche Rezeption im 18. Jahrhundert: Subversives Übersetzen am Übergang zur Frühaufklärung
Authors: Axtner-Borsutzky Anna 
Published: December 2024

ZusammenfassungAphra Behn is regarded as England’s first professional writer. Her 1688 novel Oroonoko. Or the Royal Slave. A true History deals with the English overseas territories decades before Daniel Defoe’s Robinson Crusoe (1719). It was translated into German in Hamburg in 1709 and was subsequently widely read. The translation, made by a certain Magister Vischer, remains close to the original text, but semantically changes some nuances that should be emphasised. These include the conspicuous use of the terms „Europeans“ and „European women“, which Aphra Behn does not use with such clarity. Based on this and other examples, the article suggests how the German translation of the English source text, which was still published in the seventeenth century, could be considered an indicator of the early German Enlightenment at the beginning of the eighteenth century through subversive translation.
The impact of short-term rental activity on house prices: evidence from coastal Virginia
Authors: Safari Navid , Zhang Lei , Komarek Timothy M. 
Published: December 2024

AbstractShort-term rentals such as Airbnb have grown substantially in recent years. In this study, we investigated the effect of Airbnb rentals on neighboring house prices in Hampton Roads, Virginia. We focus on three different measures of Airbnb activity to obtain more robust results. Our measures include active Airbnb listings, active listings with at least one reservation, and the sum of reservations over three different periods. Consistent with previous literature, our main results show that prior Airbnb rental activity has a positive impact on the surrounding housing sales prices. Furthermore, the localized impact diminishes over geographic space (i.e., distances greater than 500 m). We also examine how Airbnb rental activity impacts house prices across the price distribution of home sales, using quantile regression. We find that the effect is strongest for higher-priced homes, while lower-priced houses are less impacted by Airbnb rentals.
An improved calibration methodology and uncertainty assessment in measurements of microbubble size and concentration
Authors: Stigter R. , Fiscaletti D. , Elsinga G. E. , van Terwisga T. , Westerweel J. 
Published: December 2024

Abstract
Interferometric particle imaging (IPI) is used to measure both the size distribution and concentration of microbubbles (with a diameter less than 100 micron) in water. Using a new method for calibration makes it possible to obtain quantitative results for the concentration of microbubbles. The results are validated using imaging with a long-range microscope shadowgraph (LMS). Estimates of the size distribution and concentration from both IPI and LMS agree within uncertainty limits. The relative uncertainty in the IPI concentration estimation is about 10% and is mostly due to the finite number of detected bubbles. It is shown that the performance of the bubble-image detection algorithm needs to be quantified to obtain a reliable estimate of the concentration obtained with IPI.
Intravenous and oral administration of the synthetic RNA drug, TY1, reverses heart failure with preserved ejection fraction in mice
Authors: Miyamoto Kazutaka , Jones Xaviar M. , Yamaguchi Shukuro , Ciullo Alessandra , Li Chang , Coto Joshua Godoy , Tsi Kara , Anderson Jessica , Morris Ashley , Marbán Eduardo , Ibrahim Ahmed Gamal-Eldin 
Published: December 2024

AbstractTY1, a synthetic non-coding RNA (ncRNA) bioinspired by small Y RNAs abundant in extracellular vesicles (EVs), decreases cGAS/STING activation in myocardial infarction and thereby attenuates inflammation. Motivated by the concept that heart failure with preserved ejection fraction (HFpEF) is a systemic inflammatory disease, we tested TY1 in a murine model of HFpEF. Intravenous TY1, packaged in a transfection reagent, reversed the cardiac and systemic manifestations of HFpEF in two-hit obese-hypertensive mice, without inducing weight loss. The effects of TY1 were specific, insofar as they were not reproduced by a control RNA of the same nucleotide content but in scrambled order. TY1 consistently suppressed myocardial stress-induced MAP kinase signaling, as well as downstream inflammatory, fibrotic, and hypertrophic gene pathways in heart tissue. TY1 not only prevented but actually reversed key pathological processes underlying HFpEF, with no evidence of toxicity. Most noteworthy from a practical perspective, the effects of intravenous TY1 were reproduced by feeding HFpEF mice an oral micellar formulation of TY1. As the prototype for a novel class of ncRNA drugs which target cell stress, TY1 exhibits exceptional disease-modifying bioactivity in HFpEF.
Zugangswege zum minimalinvasiven Aortenklappenersatz
Authors: Krapf Christoph , Semsroth Severin , Gollmann-Tepeköylü Can , Stastny Lukas , Ruttmann-Ulmer Elfriede , Grimm Michael , Bonaros Nikolaos 
Published: December 2024

ZusammenfassungNeben dem klassischen herzchirurgischen Zugang über eine volle Sternotomie haben sich in den letzten Jahren reduzierte und minimalinvasive chirurgische Zugänge etabliert, die in einigen Zentren den konventionellen Zugang zur Aortenklappenchirurgie fast vollständig verdrängt haben. Während sich bei der partiellen Sternotomie zeigt, dass dieser Zugang der klassischen Sternotomie auch mit konventionellen Techniken nicht nachsteht, haben v. a. bei den thorakalen Zugängen technische Entwicklungen bei Aortenklappenprothesen, automatisierten Näh- und Fixationssystemen sowie peripheren Kanülierungstechniken für die Herz-Lungen-Maschine einen beträchtlichen Einfluss auf die Durchführbarkeit des Zugangsweges. Im direkten Vergleich der oberen Hemisternotomie mit dem anterolateralen Zugang zeigt sich an unserem Zentrum, dass Erstere eine geringere Mortalität, eine geringere Komplikationsrate und kürzere Perfusionszeiten aufweist. Auch sind die Durchdringung und Akzeptanz dieses Verfahrens größer. Am ehesten scheint die Weiterentwicklung des Thorakotomiezugangs im Sinne einer endoskopischen Port-Access-Operation wesentliche Vorteile für die dafür geeigneten PatientInnen zu bieten. Die PatientInnen profitieren durch minimalinvasive Verfahren v. a. von der geringeren Invasivität am knöchernen Thorax und Weichteilmantel sowie durch kleinere Narben. Dies führt nicht nur zu kosmetischen Vorteilen, sondern auch zu einer verbesserten Stabilität des Thorax und somit zu einer schnelleren Mobilisation und Rehabilitation.