bluuebunny/crossref_metadata_2025_split huggingface dataset:
Renalase gene Glu37Asp polymorphism affects susceptibility to diabetic retinopathy in type 2 diabetes mellitus
Authors: Buraczynska Monika , Gwiazda-Tyndel Karolina , Drop Bartłomiej , Zaluska Wojciech 
Published: June 2021

Abstract
Aims
Renalase (RNLS) is an enzyme with monoamine oxidase activity that metabolizes circulating catecholamines. The RNLS gene Asp37Glu missense polymorphism (rs2296545) has been associated with hypertension, cardiac hypertrophy and dysfunction, and stroke. The purpose of our study was to investigate the potential involvement of this polymorphism in the microvascular complications of type 2 diabetes (T2DM).

Methods
In this case–control study, the polymorphism was genotyped in 860 patients with T2DM and 400 healthy controls. The genotype and allele distribution was compared in subgroups of patients: with diabetic nephropathy (DN+) (n = 405) versus DN− (independently of the presence of DR) and, similarly, patients with diabetic retinopathy (DR+) (n = 328) versus DR− (independently of the presence of DN).

Results
No significant association was detected between analyzed polymorphism and DN. In contrast, the retinopathy subgroup showed a significantly higher frequency of G allele (OR 1.4, 95% CI 1.16–1.72, p = 0.0005) and GG genotype (OR 1.86, 95% CI 1.26–2.75, p = 0.001) than DR− patients. The effect of RNLS Glu37Asp polymorphism on DR remained significant after adjustments for age, gender, BMI, and duration of T2DM (p = 0.005).

Conclusions
This is the first study to investigate RNLS gene polymorphism in microvascular complications of T2DM. The results suggest that RNLS rs2296545 SNP might be considered a risk factor for diabetic retinopathy in T2DM patients. This can provide new insight into the role of renalase gene in the pathophysiology of microvascular complications of diabetes.
Optimizing the Reaction Conditions for the Formation of Fumarate via Trans-Hydrogenation
Authors: Wienands Laura , Theiß Franziska , Eills James , Rösler Lorenz , Knecht Stephan , Buntkowsky Gerd 
Published: June 2021

AbstractParahydrogen-induced polarization is a hyperpolarization method for enhancing nuclear magnetic resonance signals by chemical reactions/interactions involving the para spin isomer of hydrogen gas. This method has allowed for biomolecules to be hyperpolarized to such a level that they can be used for real time in vivo metabolic imaging. One particularly promising example is fumarate, which can be rapidly and efficiently hyperpolarized at low cost by hydrogenating an acetylene dicarboxylate precursor molecule using parahydrogen. The reaction is relatively slow compared to the timescale on which the hyperpolarization relaxes back to thermal equilibrium, and an undesirable 2nd hydrogenation step can convert the fumarate into succinate. To date, the hydrogenation chemistry has not been thoroughly investigated, so previous work has been inconsistent in the chosen reaction conditions in the search for ever-higher reaction rate and yield. In this work we investigate the solution preparation protocols and the reaction conditions on the rate and yield of fumarate formation. We report conditions to reproducibly yield over 100 mM fumarate on a short timescale, and discuss aspects of the protocol that hinder the formation of fumarate or lead to irreproducible results. We also provide experimental procedures and recommendations for performing reproducible kinetics experiments in which hydrogen gas is repeatedly bubbled into an aqueous solution, overcoming challenges related to the viscosity and surface tension of the water.
Efficacy and safety of flexible versus rigid endoscopic third ventriculostomy in pediatric and adult populations: a systematic review and meta-analysis
Authors: Boaro Alessandro , Mahadik Bhargavi , Petrillo Anthony , Siddi Francesca , Devi Sharmila , Chawla Shreya , Abunimer Abdullah M. , Feletti Alberto , Fiorindi Alessandro , Longatti Pierluigi , Sala Francesco , Smith Timothy R. , Mekary Rania A. 
Published: June 2021

AbstractEndoscopic third ventriculostomy (ETV) is a well-established surgical procedure for hydrocephalus treatment, but there is sparse evidence on the optimal choice between flexible and rigid approaches. A meta-analysis was conducted to compare efficacy and safety profiles of both techniques in pediatrics and adults. A comprehensive search was conducted on PubMED, EMBASE, and Cochrane until 11/10/2019. Efficacy was evaluated comparing incidence of ETV failure, while safety was defined by the incidence of perioperative complications, intraoperative bleedings, and deaths. Random-effects models were used to pool the incidence. Out of 1365 studies, 46 case series were meta-analyzed, yielding 821 patients who underwent flexible ETV and 2918 who underwent rigid ETV, with an age range of [5 days–87 years]. Although flexible ETV had a higher incidence of failure in adults (flexible: 54%, 95%CI: 22–82% vs rigid: 20%, 95%CI: 22–82%) possibly due to confounding due to etiology in adults treated with flexible, a smaller difference was seen in pediatrics (flexible: 36%, pediatric: 32%). Safety profiles were acceptable for both techniques, with a certain degree of variability for complications (flexible 2%, rigid 18%) and death (flexible 1%, rigid 3%) in pediatrics as well as complications (rigid 9%, flexible 13%), death (flexible 4%, rigid 6%) and intra-operative bleeding events (rigid 6%, flexible 8%) in adults. No clear superiority in efficacy could be depicted between flexible and rigid ETV for hydrocephalus treatment. Safety profiles varied by age but were acceptable for both techniques. Well-designed comparative studies are needed to assess the optimal endoscopic treatment option for hydrocephalus.
RIC in COVID-19—a Clinical Trial to Investigate Whether Remote Ischemic Conditioning (RIC) Can Prevent Deterioration to Critical Care in Patients with COVID-19
Authors: Davidson Sean M. , Lukhna Kishal , Gorog Diana A. , Salama Alan D. , Castillo Alejandro Rosell , Giesz Sara , Golforoush Pelin , Kalkhoran Siavash Beikoghli , Lecour Sandrine , Imamdin Aqeela , do Carmo Helison R. P. , Bovi Ticiane Gonçalez , Perroud Mauricio W. , Ntsekhe Mpiko , Sposito Andrei C. , Yellon Derek M. 
Published: June 2021

Abstract
Purpose
Coronavirus disease 19 (COVID-19) has, to date, been diagnosed in over 130 million persons worldwide and is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Several variants of concern have emerged including those in the United Kingdom, South Africa, and Brazil. SARS-CoV-2 can cause a dysregulated inflammatory response known as a cytokine storm, which can progress rapidly to acute respiratory distress syndrome (ARDS), multi-organ failure, and death. Suppressing these cytokine elevations may be key to improving outcomes. Remote ischemic conditioning (RIC) is a simple, non-invasive procedure whereby a blood pressure cuff is inflated and deflated on the upper arm for several cycles. “RIC in COVID-19” is a pilot, multi-center, randomized clinical trial, designed to ascertain whether RIC suppresses inflammatory cytokine production.

Methods
A minimum of 55 adult patients with diagnosed COVID-19, but not of critical status, will be enrolled from centers in the United Kingdom, Brazil, and South Africa. RIC will be administered daily for up to 15 days. The primary outcome is the level of inflammatory cytokines that are involved in the cytokine storm that can occur following SARS-CoV-2 infection. The secondary endpoint is the time between admission and until intensive care admission or death. The in vitro cytotoxicity of patient blood will also be assessed using primary human cardiac endothelial cells.

Conclusions
The results of this pilot study will provide initial evidence on the ability of RIC to suppress the production of inflammatory cytokines in the setting of COVID-19.

Trial Registration
NCT04699227, registered January 7th, 2021.
The expression of RIPK3 is associated with cell turnover of gastric mucosa in the mouse and human stomach
Authors: Cui Guanglin , Yuan Yaobo , Wang Yanan , Li Zhenfeng 
Published: June 2021

AbstractNecroptosis is a novel manner of programmed cell death and important for tissue development, homeostasis, damage, and repair. Activation of receptor-interacting protein kinase 3 (RIPK3), a key member of receptor-interacting protein family in contributing significantly to necroptosis, in tissues is a hallmark of cells dying by necroptosis. However, there are few studies that examine the expression of RIPK3 in the glandular cells of stomachs under physiological condition. We have therefore conducted this study to immunohistochemically characterize the key element of necroptosis, RIPK3, in the mouse and human stomach. Results showed that RIPK3 positive cells could be observed in the surface mucosal cells, granular cells, and lamina propria cells in both mouse and human stomach tissues. Ratios of PCNA/RIPK3 positive cells in the glandular cells were ~ 2.1 in mouse and ~ 4.15 in human sections respectively. Morphological and double immunofluorescence analysis confirmed that these RIPK3 positive cells were mucous, parietal and lamina propria cells. Our results indicate that the expression of RIPK3 in different cell types might contribute to cell turnover of gastric mucosa in the mouse and human stomach under physiological condition.
Hydraulic hysteresis of natural pyroclastic soils in partially saturated conditions: experimental investigation and modelling
Authors: Dias Ana Sofia , Pirone Marianna , Nicotera Marco Valerio , Urciuoli Gianfranco 
Published: June 2021

AbstractIn many geotechnical applications, especially in the study of weather-induced landslides, a reliable soil hydraulic characterization in unsaturated conditions is required. Currently, the experimental techniques that neglect the hydraulic hysteresis represent the greatest limitation to landslide forecasting. In this paper, a procedure to obtain an unsaturated soil hydraulic characterization on natural pyroclastic samples is proposed and verified. The approach enables the evaluation of the soil hydraulic properties along the main drying path and wetting/drying cycles to fully quantify the effects of the hydraulic hysteresis. Pyroclastic soil samples collected at a test site at Mount Faito in the Campania region (southern Italy) were tested. The experimental investigation consisted of a sequence of testing phases: a constant-head hydraulic conductivity test, a forced evaporation test followed by several wetting–drying cycles, and a drying test in a pressure plate apparatus. The hysteretic model proposed by Parker and Lenhard (1987) was adopted to fit the data, while inverse modelling of the forced evaporation tests allowed to derive the model parameters. Therefore, the main drying and wetting branches and the soil response to drying and wetting cycles from any reversal point were reproduced with the model, which suitably described the hysteretic behaviour of the pyroclastic soil under all conditions and along all paths.
Top incomes in South Africa in the twentieth century
Authors: Alvaredo Facundo , Atkinson A. B. 
Published: June 2021

AbstractThere have been important studies of recent income inequality and of poverty in South Africa, but very little is known about the long-run trends over time. There is speculation about the extent of inequality when the Union of South Africa was formed in 1910, but no hard evidence. In this paper, we provide evidence that is partial—being confined to top incomes—but which for the first time shows how the income distribution changed on a (near) annual basis from 1913 onwards. We present estimates of the shares in total income of groups such as the top 1% and the top 0.1%, covering the period from colonial times to the twenty-first century. For a number of years during the apartheid period, we have data classified by race. The estimates for recent years bear out the picture of South Africa as a highly unequal country, but allow this to be placed in historical and international context. The time series presented here will, we hope, provide the basis for detailed investigation of the impact of South African institutions and policies, past and present. But the similarity of the changes over time in top incomes across the four ex-dominions suggests that national developments have to be seen in the light of common global forces.
The Development and Validation of the Epistemic Vice Scale
Authors: Meyer Marco , Alfano Mark , de Bruin Boudewijn 
Published: June 2021

AbstractThis paper presents two studies on the development and validation of a ten-item scale of epistemic vice and the relationship between epistemic vice and misinformation and fake news. Epistemic vices have been defined as character traits that interfere with acquiring, maintaining, and transmitting knowledge. Examples of epistemic vice are gullibility and indifference to knowledge. It has been hypothesized that epistemically vicious people are especially susceptible to misinformation and conspiracy theories. We conducted one exploratory and one confirmatory observational survey study on Amazon Mechanical Turk among people living in the United States (total N = 1737). We show that two psychological traits underlie the range of epistemic vices that we investigated: indifference to truth and rigidity. Indifference manifests itself in a lack of motivation to find the truth. Rigidity manifests itself in being insensitive to evidence. We develop a scale to measure epistemic vice with the subscales indifference and rigidity. The Epistemic Vice Scale is internally consistent; has good convergent, divergent, and discriminant validity; and is strongly associated with the endorsement of misinformation and conspiracy theories. Epistemic vice explains additional variance in the endorsement of misinformation and conspiracy theories over and above demographic and related psychological concepts and shows medium to large effect sizes across outcome measures. We demonstrate that epistemic vice differs from existing psychological constructs, and show that the scale can explain individual differences in dealing with misinformation and conspiracy theories. We conclude that epistemic vice might contribute to “postfactive” ways of thinking.
New PCR-specific markers for pollen fertility restoration QRfp-4R in rye (Secale cereale L.) with Pampa sterilizing cytoplasm
Authors: Niedziela Agnieszka , Wojciechowska Marzena , Bednarek Piotr Tomasz 
Published: June 2021

AbstractPampa cytoplasmic male sterility phenomenon is used extensively in the rye hybrid breeding programs. It relies on sterilizing action of the cytoplasm resulting in non-viable pollen of female lines. The sterilizing effect is problematic for reversion, and efficient restores are needed. The most promising QTL is located on chromosome 4R, but other chromosomes may also code the trait. Advanced recombinant inbred lines formed bi-parental mapping population genotyped with DArTseq markers. Genetic mapping allowed the seven linkage groups to construct with numerous markers and represent all rye chromosomes. Single marker analysis and composite interval mapping were conducted to identify markers linked to the pollen fertility. Association mapping was used to detect additional markers associated with the trait. A highly significant QTL (QRfp-4R) that explained 42.3% of the phenotypic variation was mapped to the distal part of the long arm of the 4R chromosome. The markers localized in the QRfp-4R region achieve R2 association values up to 0.59. The homology of the 43 marker sequences to the loci responsible for fertility restoration in other species and transcription termination factor (mTERF) linked to Rf genes was established. Ten markers were successfully converted into PCR-specific conditions, and their segregation pattern was identical to that of unconverted DArTs.
Rapid and sustained decline in CXCL-10 (IP-10) annotates clinical outcomes following TNFα-antagonist therapy in hospitalized patients with severe and critical COVID-19 respiratory failure
Authors: Hachem Hilal , Godara Amandeep , Schroeder Courtney , Fein Daniel , Mann Hashim , Lawlor Christian , Marshall Jill , Klein Andreas , Poutsiaka Debra , Breeze Janis L. , Joshi Raghav , Mathew Paul 
Published: June 2021

AbstractBackground:A feedforward pathological signaling loop generated by TNFα and IFN-γ synergy in the inflamed lung, driving CXCL-10 (IP-10) and CXCL-9 chemokine-mediated activated T-cell and monocyte/macrophage tissue recruitment, may define the inflammatory biology of lethal COVID-19 respiratory failure.Methods:To assess TNFα-antagonist therapy, 18 hospitalized adults with hypoxic respiratory failure and COVID-19 pneumonia received single-dose infliximab-abda therapy 5 mg/kg intravenously between April and December 2020. The primary endpoint was time to increase in oxygen saturation to fraction of inspired oxygen ratio (SpO2/FiO2) by ≥50 compared to baseline and sustained for 48 h. Secondary endpoints included 28-day mortality, dynamic cytokine profiles, secondary infections, duration of supplemental oxygen support, and hospitalization.Findings:Patients were predominantly in critical respiratory failure (15/18, 83%), male (14/18, 78%), above 60 years (median 63 years, range 31–80), race-ethnic minorities (13/18, 72%), lymphopenic (13/18, 72%), steroid-treated (17/18, 94%), with a median ferritin of 1953 ng/ml. Sixteen patients (89%) met the primary endpoint within a median of 4 days; 14/18 (78%) were discharged in a median of 8 days and were alive at 28-day follow-up. Three deaths were attributed to secondary lung infection. Mean plasma IP-10 levels declined sharply from 9183 to 483 pg/ml at Day 3 and 146 pg/ml at Day 14/discharge. Significant Day 3 declines in IFN-$\gamma $, TNFα, IL-27, CRP, and ferritin occurred. IP-10 and CXCL-9 declines were strongly correlated among patients with lymphopenia reversal (Day 3, Pearsonr: 0.98,P-value 0.0006).Interpretation:Infliximab-abda may rapidly abrogate pathological inflammatory signaling to facilitate clinical recovery in severe and critical COVID-19.
The hidden potential of call detail records in The Gambia
Authors: Arai Ayumi , Knippenberg Erwin , Meyer Moritz , Witayangkurn Apichon 
Published: June 2021

AbstractAggregated data from mobile network operators (MNOs) can provide snapshots of population mobility patterns in real time, generating valuable insights when other more traditional data sources are unavailable or out-of-date. The COVID-19 pandemic has highlighted the value of remotely-collected, high-frequency, localized data in inferring the economic impact of shocks to inform decision-making. However, proper protocols must be put in place to ensure end-to-end user-confidentiality and compliance with international best practice. We demonstrate how to build such a data pipeline, channeling data from MNOs through the national regulator to the analytical users, who in turn produce policy-relevant insights. The aggregated indicators analyzed offer a detailed snapshot of the decrease in mobility and increased out-migration from urban to rural areas during the COVID-19 lockdown. Recommendations based on lessons learned from this process can inform engagements with other regulators in creating data pipelines to inform policy-making.
More choice for men? Marriage patterns after World War II in Italy
Authors: Battistin Erich , Becker Sascha O. , Nunziata Luca 
Published: June 2021

AbstractWe investigate how changes in the sex ratio induced by World War II affected the bargaining patterns of Italian men in the marriage market. Marriage data from the first wave of the Italian Household Longitudinal Survey (1997) are matched with newly digitized information on war casualties coming from the Italian National Bureau of Statistics. We find that men in post-war marriages were better off in terms of their spouse's education, this gain amounting to about half a year of schooling. By considering heterogeneity across provinces, we find that the effects were more pronounced in rural provinces, mountainous provinces, and provinces with a higher share of population employed in agriculture. This result suggests that in these provinces the war caused a more fundamental change in marriage patterns compared to urban, lower-lying, and less agricultural provinces where marriage markets might have been more flexible to begin with.
The earliest example of sexual dimorphism in bivalves—evidence from the astartid Nicaniella (Lower Jurassic, southern Germany)
Authors: Karapunar Baran , Werner Winfried , Fürsich Franz T. , Nützel Alexander 
Published: June 2021

AbstractProtandrous sex change (sex change from male to female) is one of the diverse sexual expressions exhibited by bivalves, but its expression in the shell is quite rare. Previous studies on living and fossil astartids suggest a relationship between protandrous sex change and the formation of crenulations on the ventral shell margin at later ontogenetic stages. Here we report the formation of such crenulations in the Early Jurassic astartid Nicaniella rakoveci (Kuhn, 1935) from the Amaltheenton Formation at Buttenheim, Franconia. This is the earliest known record of protandrous hermaphroditism in fossil bivalves, predating previous reports by at least 13 Myr. A principal component analysis of linear size measurements and Fourier shape analysis of the shell outlines revealed that the outline of Nicaniella rakoveci specimens varies from subquadrate to subcircular, but this variation is independent of the presence or absence of crenulations and therefore not associated with sex. Crenulated specimens exhibit a lower height/inflation ratio than non-crenulated ones, suggesting that the protandrous females have more inflated valves than the males. The formation of crenulations was probably related to allocation of resources for reproduction. The most likely function of the crenulations was to increase the internal shell volume in the female stage to accommodate more eggs rather than being an adaptation against predation as often assumed for other bivalves. The formation of crenulations is part of the protandrous life history and probably is controlled by a genetic mechanism that is also responsible for sex change.
Intrapopulation variation in the diet of franciscana dolphin (Pontoporia blainvillei) off southern Brazil
Authors: Bassoi Manuela , Secchi Eduardo R. , Danilewicz Daniel , Moreno Ignacio B. , Santos Roberta A. , Shepherd John G. 
Published: June 2021

AbstractThe franciscana dolphin (Pontoporia blainvillei) is a coastal dolphin endemic to the western South Atlantic Ocean. The dolphin is listed as vulnerable in the IUCN Red List, with incidental catches in gillnet fisheries the greatest conservation concern for this species. Insights into the feeding habits of this dolphin are essential to understand its distribution, movements and use of habitat, which are fundamental for effective management of the species. The feeding habits of franciscana dolphins were investigated from analyses of stomach contents of animals incidentally caught by two fishing operations from southern and northern regions of the southern Brazilian coast. In this study we investigate the existence of intrapopulation (sexual maturity and sex-related) variation in the diet of the franciscana dolphin, evaluating the spatial (northern and southern geographic areas) and seasonal influences. The analyses were based on Linear and Generalized Linear Models (LM and GLM). The majority of identified prey species were bottom-dwelling teleosts and the squidDoryteuthis sanpaulensis. The most important prey differed spatially and seasonally between northern and southern regions of the study area, and our results revealed significant differences between sexes and sexual maturity stages, mainly related to prey species sizes. This variation might indicate differences in prey selection, availability or habitat use patterns among these groups. In any case, these dietary differences are likely to minimize intraspecific competition for food resources, and/or indicate spatio-temporal variation in prey availability.