bluuebunny/crossref_metadata_2025_split huggingface dataset:
On the topochronic map of the human brain dynamics
Authors: Sorrentino P. , Petkoski S. , Sparaco M. , Troisi Lopez E. , Signoriello E. , Baselice F. , Bonavita S. , Pirozzi M.A. , Quarantelli M. , Sorrentino G. , Jirsa V. 
Published: July 2021

AbstractTwo structurally connected brain regions are more likely to interact, with the lengths of the structural bundles, their widths, myelination, and the topology of the structural connectome influencing the timing of the interactions. We introduce an in vivo approach for measuring functional delays across the whole brain using magneto/electroencephalography and integrating them with the structural bundles. The resulting topochronic map of the functional delays/velocities shows that larger bundles have faster velocities. We estimated the topochronic map in multiple sclerosis patients, who have damaged myelin sheaths, and controls, demonstrating greater delays in patients across the network and that structurally lesioned tracks were slowed down more than unaffected ones. We provide a novel framework for estimating functional transmission delays in vivo at the single-subject and single-fiber level.One-Sentence SummaryA non-invasive estimation of the individual deterministic spatio-temporal scaffold underlying the evolution of brain dynamics.
CRISPRa screening with real world evidence identifies potassium channels as neuronal entry factors and druggable targets for SARS-CoV-2
Authors: Wang Chengkun , Dinesh Ravi K. , Qu Yuanhao , Rustagi Arjun , Cousins Henry , Zengel James , Zhang Tianyi , Magazine Nicholas , Guo Yinglong , Hall Taryn , Beck Aimee , Heilbroner Lucas Miecho , Peters-Schulze Grace , Wilk Aaron , Tso Luke , Erdemic Elif Tokar , Tanudtanud Kae , Ren Sheng , Tzeng Kathy Tzy-Hwa , Wang Mengdi , Howitt Brooke , Huang Weishan , Carette Jan , Altman Russ , Blish Catherine A. , Cong Le 
Published: July 2021

AbstractAlthough vaccines for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have been successful, there are no good treatments for those who are actively infected. While SARS-CoV-2 primarily infects the respiratory tract, clinical evidence indicates that cells from sensory organs and the brain are also susceptible to infection. While many patients suffer from diverse neurological symptoms, the virus’s neuronal entry remains mysterious. To discover host factors involved in SARS-CoV-2 viral entry, we performed CRISPR activation (CRISPRa) screens targeting all 6000+ human membrane proteins in cells with and without overexpression of ACE2 using Spike-pseudotyped lentiviruses. This unbiased gain-of-function screening identified both novel and previously validated host factors. Notably, newly found host factors have high expression in neuronal and immune cells, including potassium channel KCNA6, protease LGMN, and MHC-II component HLA-DPB1. We validated these factors using replication-competent SARS-CoV-2 infection assays. Notably, the overexpression of KCNA6 led to a marked increase in infection even in cells with undetectable levels of ACE2 expression. Analysis of human olfactory epithelium scRNA-seq data revealed that OLIG2+/TUJ1+ cells--previously identified as sites of infection in COVID-19 autopsy studies-- have high KCNA6 expression and minimal levels of ACE2. The presence of KCNA6 may thus explain sensory/neuronal aspects of COVID-19 symptoms. Further, we demonstrate that FDA-approved compound dalfampridine, an inhibitor of KCNA-family potassium channels, suppresses viral entry in a dosage-dependent manner. Finally, we identified common prescription drugs likely to modulate the top identified host factors, and performed a retrospective analysis of insurance claims of ~8 million patients. This large cohort study revealed a statistically significant association between top drug classes, particularly those targeting potassium channels, and COVID-19 severity. Taken together, the potassium channel KCNA6 facilitates neuronal entry of SARS-CoV-2 and is a promising target for drug repurposing and development.
Single-cell anatomical analysis of human hippocampus and entorhinal cortex uncovers early-stage molecular pathology in Alzheimer’s disease
Authors: Davila-Velderrain Jose , Mathys Hansruedi , Mohammadi Shahin , Ruzicka Brad , Jiang Xueqiao , Ng Ayesha , Bennett David A. , Tsai Li-Huei , Kellis Manolis 
Published: July 2021

AbstractThe human hippocampal formation plays a central role in Alzheimer’s disease (AD) progression, cognitive traits, and the onset of dementia; yet its molecular states in AD remain uncharacterized. Here, we report a comprehensive single-cell transcriptomic dissection of the human hippocampus and entorhinal cortex across 489,558 cells from 65 individuals with varying stages of AD pathology. We transcriptionally characterize major brain cell types and neuronal classes, including 17 glutamatergic and 8 GABAergic neuron subpopulations. Combining evidence from human and mouse tissue-microdissection, neuronal cell isolation and spatial transcriptomics, we show that single-cell expression patterns capture fine-resolution neuronal anatomical topography. By stratifying subjects into early and late pathology groups, we uncover stage-dependent and cell-type specific transcriptional modules altered during AD progression. These include early-stage cell-type specific dysregulation of cellular and cholesterol metabolism, late-stage neuron-glia alterations in neurotransmission, and late-stage signatures of cellular stress, apoptosis, and DNA damage broadly shared across cell types. Late-stage signatures show signs of convergence in hippocampal and cortical cells, while early changes diverge; highlighting the relevance of characterizing molecular pathology across brain regions and AD progression. Finally, we characterize neuron subregion-specific responses to AD pathology and show that CA1 pyramidal neurons are the most transcriptionally altered while CA3 and dentate gyrus granule neurons the least. Our study provides a valuable resource to extend cell type-specific studies of AD to clinically relevant brain regions affected early by pathology in disease progression.
High-density transposon mutagenesis in Mycobacterium abscessus identifies an essential penicillin-binding lipo-protein (PBP-lipo) involved in septal peptidoglycan synthesis and antibiotic sensitivity
Authors: Akusobi Chidiebere , Benghomari Bouchra S. , Zhu Junhao , Wolf Ian D. , Singhvi Shreya , Dulberger Charles L. , Ioerger Thomas R. , Rubin Eric J. 
Published: July 2021

AbstractMycobacterium abscessus (Mab) is a rapidly growing non-tuberculous mycobacterium (NTM) that causes a wide range of infections. Treatment of Mab infections is difficult because the bacterium is intrinsically resistant to many classes of antibiotics. Developing new and effective treatments against Mab requires a better understanding of the unique vulnerabilities that can be targeted for future drug development. To achieve this, we identified essential genes in Mab by conducting transposon-sequencing (TnSeq) on the reference Mab strain ATCC 19977. We generated ∼51,000 unique transposon mutants and used this high-density library to identify 362 essential genes for in vitro growth. To investigate species-specific vulnerabilities in Mab, we further characterized MAB_3167c, a predicted penicillin-binding-lipoprotein (PBP-lipo) that is essential in Mab and non-essential in Mycobacterium tuberculosis (Mtb). We found that PBP-lipo primarily localizes to the subpolar region and later to the septum as cells prepare to divide. Depletion of Mab PBP-lipo causes cells to elongate, develop ectopic branches, and form multiple septa. Knockdown of PBP-lipo along with PbpB, DacB1, and a carboxypeptidase, MAB_0519 lead to synergistic growth arrest. In contrast, these genetic interactions were absent in the Mtb model organism, Mycobacterium smegmatis, indicating that the PBP-lipo homologs in the two species exist in distinct genetic networks. Finally, repressing PBP-lipo sensitized the reference strain and 11 Mab clinical isolates to several classes of antibiotics, including the β-lactams, ampicillin and amoxicillin by greater than 128-fold. Altogether, this study presents PBP-lipo as a key enzyme to study Mab specific processes in cell wall synthesis and importantly positions PBP-lipo as an attractive drug target to treat Mab infections.
Institutional pressures, environmental management practices, firm characteristics and environmental performance
Authors: Bananuka Juma , Bakalikwira Lasuli , Nuwagaba Patience , Tumwebaze Zainabu 
Published: July 2021

Purpose
The purpose of this paper is twofold: to establish the contribution of institutional pressures, environmental management practices and firm characteristics to environmental performance; and to establish whether environmental management practices mediate the relationship between institutional pressures and environmental performance.

Design/methodology/approach
Using a cross-sectional design, data were collected through a questionnaire survey of 303 manufacturing firms in Uganda. Data were analyzed using Statistical Package for Social Sciences and MedGraph program (Excel version).

Findings
Both environmental management practices and institutional pressures are significant predictors of environmental performance. Results further suggest that environmental management practices partially mediate the relationship between institutional pressures and environmental performance. Variables that represent firm characteristics are not significantly associated with environmental performance.

Originality/value
This study provides an initial empirical evidence on the mediating role of environmental management practices in the relationship between institutional pressures and environmental performance. It also enhances our understanding of the contribution of individual dimensions of environmental management practices and institutional pressures to environmental performance using evidence from an emerging economy setting.
Commitment in an organizational context: a SET-theoretic approach that provides management tools
Authors: Curado Carla , Jerónimo Helena Mateus , Henriques Paulo Lopes , Tavares Marta 
Published: July 2021

PurposeGrounded on the social exchange theory (SET), this paper aims to analyze the different networks of conditions that lead to the presence and absence of total commitment, in its affective, continuance and normative dimensions. Six conditions – competencies development, empowerment, work-family conflict, trust, transformational leadership and seniority – are studied to explore and explain the exchanges and reciprocity relationships between organizations and employees.Design/methodology/approachUsing a sample of 546 employees from Portuguese large companies, the study uses the fuzzy-set qualitative comparative analysis (fsQCA) to explore how causal conditions jointly link to the outcome of organizational commitment.FindingsThe results show more causal configurations leading to the most favorable dimensions of commitment (affective, normative and the absence of continuance commitment), which is a positive sign for organizations. Employees' seniority stands out in all types of commitment, reflecting the affective and emotional themes in Portuguese culture.Originality/valueThis study makes an original contribution to the literature on organizational commitment by showing the alternative complementarity of the conditions in the context of the SET, only possible through fsQCA. It also provides inputs for practitioners who can adopt strategies and measures to develop the paths that lead to successful outcomes (presence of commitment) and avoid those that lead to undesired ones (absence of commitment).
Stagnation point flow toward an exponentially shrinking sheet in a hybrid nanofluid
Authors: Waini Iskandar , Pop Ioan , Abu Bakar Sakhinah , Ishak Anuar 
Published: July 2021

Purpose
This paper aims to investigate the radiation and magnetohydrodynamic effect on the flow toward a stagnation point of an exponentially shrinking sheet in a hybrid nanofluid.

Design/methodology/approach
The governing partial differential equations are transformed into a set of similarity equations and are then solved numerically using the boundary value problem solver, bvp4c, available in MATLAB software. The effects of several physical parameters on the flow and the thermal characteristics of the hybrid nanofluid are analyzed and discussed.

Findings
Numerical results clarify that the dual solutions arise for the shrinking case (λ < 0). The critical values expand for the stronger magnetic field. Besides, the skin friction and the heat transfer coefficients enhance with the rise of the magnetic field and the hybrid nanoparticles. The heat transfer rate increases by 10.11% for the nanofluid and 28.69% for the hybrid nanofluid compared to the regular fluid. In addition, the presence of radiation gives a higher heat transfer rate. Using the stability analysis, it is found that the first solution is stable, and the second solution is unstable, over time.

Originality/value
The stagnation point flow problem has been widely studied for the flow over a stretching sheet, but only limited findings can be found for the flow over a shrinking sheet. Therefore, the present study considers the problem of the stagnation point flow over a shrinking sheet in a Cu-Al2O3/water hybrid nanofluid with the effects of magnetic field and thermal radiation. The dual solutions of the hybrid nanofluid flow over a shrinking sheet are obtained. Further analysis shows that only one of the solutions is stable and thus physically reliable as time evolves.
Improving student satisfaction and learning outcomes with service quality of online courses: evidence from Thai and Indonesian higher education institutions
Authors: Darawong Chonlatis , Widayati Ani 
Published: July 2021

PurposeThe purpose of this article is to compare Thai students with Indonesian students in regard to the impact of four service quality dimensions on student satisfaction and the learning outcomes of online courses.Design/methodology/approachSamples were 352 Indonesian and 380 Thai students who were in bachelor's degree programmes and experienced online course services.FindingsFor Thai samples, however, the strongest dimension of service quality affecting student satisfaction is reliability, followed by responsiveness and competence, accordingly. For Indonesian samples, the strongest effect service quality dimension affecting student satisfaction is empathy, followed by responsiveness, competence and reliability.Research limitations/implicationsFirst, the sample consisted of online course students from two countries, Thailand and Indonesia. The findings may not be generalized to other countries where teaching and learning cultures are largely different. Second, four service quality dimensions were examined for their influence on student satisfaction, which consequently affects learning outcomes.Practical implicationsFirstly, instructors should strictly follow what they promise to their students. Secondly, a communication network should be developed to minimize possible misunderstandings. Thirdly, faculty administrations should provide training programmes for online instructors to enhance their competence. Lastly, online instructors need appropriate support mechanisms for technical issues and mishaps that students may face in technology-enhanced learning environments.Originality/valueThis study performs an original survey conducted in Thailand and Indonesia and reports the results based on the data analysis and the interpretation of research findings.
COVID-19 complications and economic recovery of China: guidelines for other economies
Authors: Soomro Bahadur Ali , Mangi Shah Nawaz , Shah Naimatullah 
Published: July 2021

PurposeCoronavirus (COVID-19) brought massive economic damage throughout the globe. Economic as well as social set-ups have enormously collapsed. The present paper underlines some economic complications of China during COVID-19. The study also sheds light on initiatives taken by the government of China to fight against such a damaging pandemic.Design/methodology/approachThe study employed secondary research or desk research that involves existing data. The data were collected from reliable sources, described below. The data were summarized and organized to increase the overall efficacy and reliability of the research.FindingsThe study finds that China has confronted many downturns in export and import, GDP decline, blockage of markets and production evaluation. To recover from these unfavorable and adverse circumstances, China has recovered its economy by introducing digital transformation, effective legislations, A V-shaped recovery and A U-shaped recovery in manufacturing and services.Practical implicationsChina's steps and initiatives to invigorate economic developments during a pandemic would be beneficial for other economies facing the severe challenges of economic development, especially after COVID-19.Originality/valueThe present study is one of the rare studies of the domain that offers the guidelines to the policymakers and economists of developing countries to develop their policies regarding the digital economy to face the traditional market's challenges.
The relationship between management entrenchment and financial statement fraud
Authors: Seifzadeh Maryam , Rajaeei Raha , Allahbakhsh Arezao 
Published: July 2021

Purpose
This study aims to assess the relationship between managerial entrenchment and the chance of fraud in financial statements, which are the only available source for shareholders’ decisions, so their accuracy and reliability are of great importance. Hence, the realization of contributing factors to preventing financial information distortion is vital. Moreover, managerial entrenchment on the chance of fraud in the company’s upcoming years has also been analyzed.

Design/methodology/approach
The factor analysis of five variables [chief executive officer (CEO) duality, managerial ownership, board independence, board compensation and CEO tenure] is used for management entrenchment. To examine the hypothesis testing, multivariate regressions, feasible general least squares regression and Logit model regression are used. The statistical sample under study in this paper includes 1,122 year-company observations during 2013–2018 and Beneish’s (1999) model is used for evaluating fraud.

Findings
The study results show a negative and significant relationship between management entrenchment and the chance of fraud in financial statements. That means managers with a higher degree of managerial entrenchment are more likely to create value and acquire wealth for the firm, and that causes them not to waste and waste the firm resources through enhancing the supervisory mechanisms. Moreover, the study results also show that improving and strengthening management entrenchment will lower the upcoming years’ fraud condition.

Originality/value
The current paper is the first time that the relationship between managerial entrenchment and financial statement fraud is assessed within a study. The results of the paper help the beneficiaries and shareholders realize different aspects of management entrenchment. That means managers’ power and authority can be used to make shareholders’ interests, but they can hinder misuse and fraud.
Does cluttered social media environment hurt advertising effectiveness? The moderation of ad types and personalization
Authors: Jung A-Reum , Heo Jun 
Published: July 2021

PurposeThe purpose of this study is to figure out the factors (i.e. ad type and ad personalization) that diminish the detrimental advertising clutter effects in terms of ad attention and ad clicks.Design/methodology/approachTo fulfill the purpose, an eye-tracking study using real-time Facebook accounts of the participants was conducted.FindingsThe findings suggest that not all types of ad format face attentional competition. Consumers have a tendency to selectively care of native advertising area, where clutter becomes a significant issue. Additionally, personalized advertising is beneficial for attracting consumer attention regardless of the clutter level.Originality/valueThis less-artificial study setting with an eye-tracker makes up for the findings from previous ad clutter studies based on self-reported data; this study was able to observe real-life interaction between consumers and social media. The personalized native format may benefit advertisers in grabbing more attention. However, the careful use of native ads is recommended, because excessive ads could increase the attentional competition among native ads.
Understanding innovativeness and commitment to sustainable service practices
Authors: Marín-García Antonio , Gil-Saura Irene , Ruiz-Molina Maria-Eugenia 
Published: July 2021

PurposeThe purpose of this study is two-fold. First, to examine the relationship between the retailer’s innovativeness and sustainability from the customer standpoint. Second, to assess the impact of the retailer’s innovativeness and commitment toward sustainability as perceived by the consumer on store image and store equity.Design/methodology/approachTo achieve this objective, a theoretical model is proposed based on the literature that is tested through an empirical study conducted on a sample of 510 customers of three grocery retail formats – i.e. hypermarkets, supermarkets and discount stores.FindingsThe results obtained confirm the hypotheses proposed and, consequently, both innovation and sustainability emerge as key elements in the development of store equity through store image.Originality/valueThis study allows to draw a set of managerial recommendations for food retailers based on the benefits of investing in innovative processes, that boosted by the implementation of innovative solutions, assist in the development of sustainable practices, thus allowing improvements in store image and store brand equity.
Interval type-2 fuzzy inference-based failure mode and effect analysis model in a group decision-making setting
Authors: Gölcük İlker 
Published: July 2021

PurposeThis paper proposes an integrated IT2F-FMEA model under a group decision-making setting. In risk assessment models, experts' evaluations are often aggregated beforehand, and necessary computations are performed, which in turn, may cause a loss of information and valuable individual opinions. The proposed integrated IT2F-FMEA model aims to calculate risk priority numbers from the experts' evaluations and then fuse experts' judgments using a novel integrated model.Design/methodology/approachThis paper presents a novel failure mode and effect analysis (FMEA) model by integrating the fuzzy inference system, best-worst method (BWM) and weighted aggregated sum-product assessment (WASPAS) methods under interval type-2 fuzzy (IT2F) environment. The proposed FMEA approach utilizes the Mamdani-type IT2F inference system to calculate risk priority numbers. The individual FMEA results are combined by using integrated IT2F-BWM and IT2F-WASPAS methods.FindingsThe proposed model is implemented in a real-life case study in the furniture industry. According to the case study, fifteen failure modes are considered, and the proposed integrated method is used to prioritize the failure modes.Originality/valueMamdani-type singleton IT2F inference model is employed in the FMEA. Additionally, the proposed model allows experts to construct their membership functions and fuzzy rules to capitalize on the experience and knowledge of the experts. The proposed group FMEA model aggregates experts' judgments by using IT2F-BWM and IT2F-WASPAS methods. The proposed model is implemented in a real-life case study in the furniture company.