bluuebunny/crossref_metadata_2025_split huggingface dataset:
An Initial-Controlled Double-Scroll Hyperchaotic Map
Authors: Li Yongxin , Li Chunbiao , Liu Sicong , Lei Tengfei , Jiang Yicheng 
Published: December 2021

Abstract
Initial condition-dominated offset boosting provides a special channel to arrange coexisting orbits. Due to the nonlinearity and inherent periodicity, sinusoidal function is often introduced into a dynamical system for multistability design. In this paper, an initial-controlled double-scroll hyperchaotic map is constructed based on two sine functions. Four patterns of the double-scroll hyperchaotic orbits are found as 0-degree, 90-degree, 45-degree and 135-degree. Consequently, different modes for attractor growing are demonstrated. Finally, hardware experiments based on STM32 are carried out to verify the theoretical analysis and numerical simulation.
Outcomes of Side to End versus End to End Colorectal Anastomosis in Non-emergent Sigmoid and Rectal Cancers. Randomized Controlled Clinical Trial.
Authors: Tamer.A.A.M.Habeeb  , Mohammad Hatem , TamerWasefy  , Mansour Mohamed Ibrahim 
Published: December 2021

Abstract
Purpose

The outcomes of open side-to-end colorectal anastomosis versus open end to end colorectal anastomosis in non-emergent sigmoid and rectal cancers open surgery in adults were compared.
Methods

A randomized controlled trial on individuals with sigmoid and rectal cancers was conducted between September 2016 and September 2018.
Results

The majority of the participants in the study were between the ages of 50 and 70 years, with a mean age of 62.58±12.3 years in the side-to-end anastomotic group (SEA group = group A = antegrade approach) and 61.03±13.98 years in the end-to-end anastomotic group (EEA group = group B = retrograde approach), respectively. Except for the operative time, intraoperative data revealed no significant differences between the studied groups, and the SEA group revealed that the mean anastomotic time was significantly shorter. Perioperative blood loss, length of stay, reoperation, inpatient death, infection, and bleeding were all revealed to be significantly associated with leakage in univariate analysis. In a multivariate analysis of anastomotic leaks, infection was the only independent predictor. There is a statistically significant change regarding the range of bowel frequency in the EEA group only (p = 0.04). There is a statistically significant difference regarding incontinence for Flatus in the SEA group only (p = 0.00). A statistically significant change in both groups regards incontinence for liquid stools (p = 0.00) and clustering of stools (p = 0.00 and p = 0.043). The quality of life (QOL) in the SEA group significantly dropped at 6 months and returned to baseline after that as regards PWB, FWB, and CCS with no difference as regards SWB & EWB, while in the EEA group, the exact change happened only as regard PWB & FWB, but SWB and CCS percentage did not return to baseline.
Conclusion

The SEA group offers a safe and approach alternative to the EEA group.
Camel-related Major Vascular Injuries: A 20-years’ Experience
Authors: Abu-Zidan Fikri M. , Abdel-Kader Saleh , Mousa Hussam , Aiyan Ahmad Al , Baguneid Mohamed S. , Jawas Ali 
Published: December 2021

Abstract
Background: Majority of animal-related injuries in United Arab Emirates are caused by camels. We aimed to study the anatomy, biomechanism and outcome of major camel-related human vascular injuries and correlate them to the camel anatomy and behavior.Methods: We retrospectively studied all patients who were admitted to Al-Ain Hospital with camel-related major vascular injury during January 2001 to January 2020. A special protocol was designed to collect the data. An adult 7-year-old male camel skull was prepared and web-based video clips of camel bites to humans were analysed to have an in-depth understanding of the biomechanism of injury.Results: Seven patients were studied, all were males having a median age of 26 years. Five out of six bite injuries (83%) occured during the camel rutting season. The injuries were severe and life threatening. Four involved the carotid artery, one the femoral artery and vein, one the external iliac vein and one the aorta which was due to a fall from a camel. Although the standard of surgical care was high, the outcome was poor. Six patients were admitted to the ICU for a median of 5 days. One patient died, one was vegetative, and one had arm paralysis. The elongated jaws of the camel having its biting component at the front, and the strong masseter muscles at the back increases the strength of its bite grip. Each bite causes four small elliptical wounds of the canine teeth which resembles two stab wounds of 8 cm long, penetrating deeply and injuring major vessels. Aggressive camels bite repeatedly to kill their victims.Conclusions: Camel-related vascular injuries have a poor clinical outcome. Although the basis of their surgical management is similar to other vascular injuries, their poor outcome is related to the aggressiveness of the attacking camel, the anatomy of its jaw, and its intention to kill. Neck wounds of camel bites can be closed primarily after debridement. Studying these unusual injuries gives an insight into the “one globe one health approach” in which humans interacts with the animals and the environment around them. It important to understand the camel behavior for injury prevention.
Human Blood-Derived Lymphoblastoid Cybrids for Assessment of Mitochondrial tRNAs Deficiency by MTO1 Silence
Authors: Chen Chao , Wang Qi , Aishanjiang Maerhaba , Wei Yuping , Gao Zewen , Chen Ye , Lei Zhixian , Ye Shixin 
Published: December 2021

Abstract
Background: Mutations in the mitochondrial translation optimization 1 (MTO1) gene can cause hypertrophic cardiomyopathy. Although the functional role of MTO1 deficiency in certain cells is gradually confirmed, the phenotype of MTO1 deficiency in a lymphoblastoid cybrid line is not yet reported. In this study, we characterized changes of mitochondrial function in MTO1 silenced cybrid cells derived from human lymphoblastoids, immature white blood cells that give rise to lymphocytes. Results: We showed that MTO1 silence decreased the levels of 2-thiourylation of mitochondrial tRNALys, tRNAGlu, and tRNAGln, nevertheless, the aminoacylation efficiency of tRNALys and the steady state of mitochondrial tRNAs were elevated. These aberrant tRNA changes caused a significant decrease in protein levels of oxidative phosphorylation complex subunits including complex I, III, IV and V. Furthermore, dysfunctional mitochondria promoted apoptosis in stress, evidenced by elevated ratios of apoptotic cells and increased levels of apoptosis-activated proteins in the MTO1 knockdown cell lines, as compared to the controls. Conclusions: Our data provide new insights into the important functional role of MTO1 in lymphoblastoid mitochondria. We envision the cybrid cell line approach we have established provides an alternative model for the cardiac tissue of high-energy demands characteristics, and they hold promises for the diagnosis and drug screening for the therapeutic agents of hypertrophic cardiomyopathy caused by MTO1 dysfunction.
SNP-based Genetic Structure of Tunisian Durum Wheat Landraces and Distribution of the Resistance to Septoria Tritici Blotch
Authors: Ouaja Maroua , Bahri Bochra , Ferjaoui Sahbi , Medini Maher , Sripa Udupa . , Hamza Sonia 
Published: December 2021

Abstract
Background: Septoria tritici blotch (STB) has marked durum wheat production worldwide. This fungal disease is until today a challenge for farmers, researchers and breeders all united in the aim of reducing its damage and improving wheat resistance. Tunisian durum wheat landraces were reported to be valuable genetic resources for resistance to biotic and abiotic stresses and are therefore prominently deployed in breeding programs to develop new varieties adapted to fungal diseases as STB and to climate change constraints overall.Results: A total of 366 local durum wheat accessions were assessed for resistance to two virulent Tunisian isolates of Zymoseptoria tritici Tun06 and TM220 under field conditions. Population structure analysis of the durum wheat accessions, performed with 286 polymorphic SNPs (PIC >0.3) covering the entire genome, identified three genetic subpopulations (GS1, GS2 and GS3) with 22% of admixed genotypes. Interestingly, all of the resistant genotypes were among GS2 or admixed with GS2. Conclusions: This study revealed the population structure and the genetic distribution of the resistance to Z. tritici in the Tunisian durum wheat landraces. The grouping pattern of accessions appear to be associated, to some extent, with the geographical pattern of the landraces. We suggested that GS2 accessions were mostly introduced from eastern Mediterranean populations, unlike GS1 and GS3 that originated from the west. Resistant GS2 accessions belonged to landraces Taganrog, Sbei glabre, Richi, Mekki, Badri, Jneh Khotifa and Azizi. Furthermore, we suggested that admixture contributed to transmit STB resistance from GS2 resistant landraces to initially susceptible landraces such as Mahmoudi (GS1), but also resulted in the loss of resistance in the case of GS2 suscpetible Azizi and Jneh Khotifa accessions.
Gene expression trend changes in breast cancer populations over two decades: insights from The Cancer Genome Atlas database
Authors: wu jinbo , Hu Taobo , wang shu 
Published: December 2021

Abstract
Background
Breast cancer has remained the most common malignancy in women over the past two decades. As lifestyle and living environments have changed, alterations to the disease spectrum have inevitably occurred in this time. As molecular profiling has become a routine diagnostic and objective indicator of breast cancer etiology, we analyzed changes in gene expression in breast cancer populations over two decades using The Cancer Genome Atlas (TCGA).
Methods
We performed Heatmap and Venn diagram analyses to identify constantly up- and down-regulated genes in this cohort. We used Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses to visualize associated functional pathways.
Results
We determined that three oncogenes, PD-L2, ETV5, and MTOR and 113 long intergenic non-coding RNAs (lincRNAs) were constantly up-regulated, whereas two oncogenes, BCR and GTF2I, one tumor suppression gene (TSG) MEN1, and 30 lincRNAs were constantly down-regulated. Up-regulated genes were enriched in “focal adhesion” and “PI3K-Akt signaling” pathways, et al, and down-regulated genes were significantly enriched in “metabolic pathways” and “viral myocarditis”. Eight up-regulated genes exhibited doubled or higher expression, and the expression of three down-regulated genes was halved or lowered and correlated with long-term survival.
Conclusions
In this study, we determined that genes and molecular pathways are constantly changing, importantly, some altered genes were associated with prognostics and are potential therapeutic targets, suggesting molecular typing technologies must keep pace with this dynamic situation.
Association of ACE2 Polymorphisms and Derived Haplotypes with Obesity and Hyperlipidemia in Female Spanish Adolescents
Authors: Lumpuy-Castillo J. , Vales-Villamarín C. , Mahíllo-Fernández I. , Pérez-Nadador I. , Soriano-Guillén L. , Lorenzo Oscar , Garcés C. 
Published: December 2021

Abstract
Background
In the cardiovascular (CV) system, overactivation of the angiotensin converting enzyme (ACE) may trigger deleterious responses derived from angiotensin (Ang)-II, which can be attenuated by stimulation of ACE2 and subsequent Ang-(1-7) metabolite. However, ACE2 exhibits a high degree of genetic polymorphism that may affect its structure and stability, interfering with these cardioprotective actions.
Methodology:
Five ACE2-single nucleotide polymorphisms (SNP); rs4646188, rs2158083, rs233575, rs879922, and rs2074192, previously related with CV risk factors, were analyzed in a general population of adolescents and tested for potential associations with anthropometric and plasma parameters.
Results
Girls (n=461) exhibited lower rates of overweight and obesity, blood pressure, and glycemia than boys (n=412), though increased plasma lipids. The triglycerides (TG)/HDL-C ratio was, however, lower in females. Interestingly, only in girls, the occurrence of overweight/obesity was associated with the SNPs rs879922 [OR 1.67 (1.02-2.75)], rs233575 [OR 1.98 (1.21- 3.22)] and rs2158083 [OR 1.67 (1.04-2.68)]. Also, their highest levels of TG were linked to rs879922, rs233575, and rs2158083, and the highest TG/HDL-C ratio was assocated with rs879922 and rs233575. The upper levels of TC and LDL-C was associated with rs2074192 and rs2158083. Furthermore, the established cut-off level for TG ≥ 90 mg/dl was related with rs879922 [OR 1.78 (1.06-2.96)], rs2158083 [OR 1.75 (1.08-2.82)], and rs233575 [OR 1.62 (1.00 -2.61)]. The cut-off level for TC ≥ 170 mg/dl was associated with rs2074192 [OR 1.54 (1.04-2.28) and rs2158083 [OR 1.53 (1.04-2.25)]. In addition, the haplotype (C-G-C) derived from rs879922-rs2158083-rs233575 was related with higher prevalence of overweight/obesity and TG elevation.
Conclusion
The expression and activity of ACE2 may be essential for CV homeostasis. Interestingly, ACE2-SNPs rs879922, rs233575, rs2158083 and rs2074192, and haplotype (C-G-C) of the three former could induce vulnerability to obesity and hyperlipidemia in women. Thus, these SNPs might be used as predictive biomarkers for CV diseases and as molecular targets for CV therapy.
Conditional Knockout of PDK1 in Osteoclasts Suppressed Osteoclastogenesis and Ameliorated Prostate Cancer-Induced Osteolysis
Authors: Zhang Yanan , Nong Haibin , Bai Yiguang , Zhou Quan , Zhang Qiong , Liu Mingfu , Liu Pan , Zeng Gaofeng , Zong Shaohui 
Published: December 2021

Abstract
Background: The development and maintenance of normal bone tissue is supported by balanced communication between osteoblasts and osteoclasts. The invasion of cancer cells disrupts this balance, leading to osteolysis. As the only bone resorption cells in vivo, osteoclasts play important roles in cancer-induced osteolysis. However, the function of 3-phosphoinositide–dependent protein kinase-1 (PDK1) in osteoclast resorption remains unclear. Methods: In our study, we used a receptor activator of nuclear factor-kappa B (RANK) promoter‐driven Cre‐LoxP system to conditionally delete the PDK1 gene in osteoclasts in mice. We investigated the impact of Osteoclast‐specific knockout of PDK1 on prostate cancer-induced osteolysis. Bone marrow-derived macrophage cells (BMMs) were extracted and induced to differentiate osteoclasts in vitro to examine the function of PDK1 in osteoclasts.Results: In this study, we found that PDK1 conditional knockout (cKO) mice exhibited smaller body sizes when contrasted with the wild-type (WT) mice. Moreover, deletion of PDK1 in osteoclasts ameliorated osteolysis and reduced bone resorption markers in the murine model of prostate cancer-stimulated osteolysis. In vivo, we discovered that osteoclast‐specific knockout of PDK1 suppressed RANKL-stimulated bone resorption function, osteoclastogenesis, and osteoclast-specific gene expression (Ctsk, TRAP, MMP-9, NFATc1). Western blot analyses of RANKL-induced signaling pathways showed that conditional knockout of PDK1 in osteoclasts inhibited the early nuclear factor κB (NF-κB) activation, which consequently suppressed the downstream induction of NFATc1. Conclusion: These findings demonstrated that PDK1 performs an instrumental function in osteoclastogenesis and prostate cancer-induced osteolysis by modulating the PDK1/AKT/NF-κB signaling pathway.
Simulation-optimization framework in urban flood management for historic and climate change scenarios
Authors: Madhuri R. , Sarath Raja Y. S. L. , Srinivasa Raju K. 
Published: December 2021

Abstract
A simulation-optimization framework is established by integrating Hydrologic Engineering Center Hydraulic Modeling System (HEC-HMS) for computation of runoff, siting tool EPA System for Urban Storm-water Treatment and Analysis INtegration (EPA-SUSTAIN) for placement of Best Management Practices (BMPs), and Binary Linear Integer Programming (BLIP) for runoff reduction. The framework is applied to an urban catchment, namely Greater Hyderabad Municipal Corporation (GHMC). The rainfall-runoff analysis was conducted for extreme rainfalls for historic (2016) and future events in 2050 and 2064 under Representative Concentration Pathways (RCPs) 6.0 and 8.5. The simulation-optimization approach in the historic scenario yielded 495,607 BMPs occupying 76.99 km2 resulting in runoff reduction of 21.54 mm (198.76–177.22 mm). Achieved runoff reduction is 38.72 (428.35–389.63 mm) and 55.03 (602.65–547.62 mm), respectively, for RCPs 6.0 and 8.5, which could meet the water demands of GHMC for 10.33 and 11.53 days. Impacts of 10 different BMP configurations of varying costs (10–70%) and pollutant load reductions (0–3%) on runoff reduction are accomplished as part of sensitivity analysis.
Synthesized NaA Nanozeolite as a Catalyst for the Preparation of 3-amino imidazo[1,2-a]pyridines under Solvent-Free Conditions
Authors: Azargashb Sara , Sarvary Afshin , Hassaninejad-Darzi Seyed Karim 
Published: December 2021

Abstract:The present study explores a new method for the fabrication of NaA nanozeolite as a simple and efficient catalyst for producing 3-aminoimidazo [1,2-a] pyridines via the 3-component reaction of aldehydes, 2-aminoperidines and isocyanides under solvent-free conditions. The production of the organic template-free (OTF) NaA nanozeolite was performed at room temperature. The prepared nanozeolite was identified by X-ray diffraction (XRD), Fourier Transform infrared spectroscopy (FT-IR), Field Emission Scanning Electronic Microscopy (FESEM), N2 sorption isotherm and Particle Size Analysis (PSA). The particle sizes of synthesized spherical NaA nanozeolite were under 100 nm via the FESEM method. BET surface area, total pore volume, and mean pore diameter of the created sample were attained to be 362 m2g-1, 0.44 cm3 g-1 and 5.9 nm, respectively. The developed method has some advantages, such as OTF production of NaA nanozeolite, a simple synthesis method with short reaction time and easy separation using filtration, and the ability to recycle and reuse the catalyst several times without reducing its efficiency.
COVID-19 Vaccination in Children: An Open Question
Authors: Bardanzellu Flaminia , Campagnani Giuseppe , Pintus Maria Cristina , Fanos Vassilios , Marcialis Maria Antonietta 
Published: December 2021

Background:
A safe and effective vaccine represents the best way to control the
COVID-19 pandemic, which has caused more than 4 million deaths to date. Several vaccines have
now been approved worldwide, depending on the country. Being administered to healthy people,
anti-SARS-CoV-2 vaccines must meet high safety standards, and this is even more important
among the pediatric population in which the risk of developing severe disease is significantly lower
than adults. However, vaccination of the pediatric population could help in reducing viral spread in
the whole population.

Objective:
Our narrative review analyzes and discusses the currently available literature on the advantages
and disadvantages of COVID-19 vaccination in the pediatric population.

Methods:
A bibliographic research was conducted through Pubmed, Read, and Scopus using
COVID-19, SARS-CoV-2, immunization, antibody, COVID-19 vaccine efficacy, COVID-19
vaccine safety, children, adolescents, MIS-C, adverse effects as keywords.

Results:
Although children are less susceptible to COVID-19 infection, they can develop serious
consequences, including multi-inflammatory syndrome. However, any vaccine-related side effects
should be evaluated before administering vaccination to children while ensuring complete safety.
To date, adverse effects are reported in adolescents and young adults following vaccination; however,
these are mostly isolated reports.

Conclusions:
Further investigation is needed to establish whether there is indeed a cause-and-effect
relationship in the development of vaccine-related adverse effects. However, to date, COVID-19
vaccination is recommended for children and adolescents older than 12 years of age. However, this
question is still under debate and involves ethical, political, and social issues.
Hepatic Lipid Metabolism Disorder and Atherosclerosis
Authors: Zhang Sen , Hong Fenfang , Ma Chen , Yang Shulong 
Published: December 2021

:
Lipid metabolism disorder plays a fundamental role in the pathogenesis of atherosclerosis. As the largest metabolic organ of the human body, liver has a key role in lipid metabolism by influencing fat production, fat decomposition, and the intake and secretion of serum lipoproteins. Numerous clinical and experimental studies have indicated that the dysfunction of hepatic lipid metabolism is closely tied to the onset of atherosclerosis. However, the identity and functional role of hepatic lipid metabolism responsible for these associations remain unknown. This review presented that cholesterol synthesis, cholesterol transport, and the metabolism of triglyceride, lipoproteins, and fatty acids are all associated with hepatic lipid metabolism and atherosclerosis. Moreover, we also discussed the roles of gut microbiota, inflammatory response, and oxidative stress in the pathological association between hepatic lipid metabolism and atherosclerosis. These significant evidences support strongly that hepatic lipid metabolism disorders may increase the risk of atherosclerosis.
Fabrication of Cefixime Nanoparticles Loaded Films and their Ex Vivo Antimicrobial
Effect on Periodontitis Patient’s Saliva
Authors: Parmar Rakeshkumar , Salman M Mohammad , Chauhan Payal 
Published: December 2021

Aim:
This study was designed to prepare and evaluate cefixime-loaded nanoparticles
containing low molecular weight chitosan films for the enhanced topical treatment of periodontitis.

Methods:
To fabricate the enhanced antimicrobial films, a nanoprecipitation method for cefixime
nanoparticles followed by a solvent evaporation method for these nanoparticles loaded films were
adopted in this study. Nine batches of nanoparticles (NPs) with different concentrations of ethyl cellulose and polyvinyl alcohol were prepared and evaluated. Furthermore, nine batches of optimized
NPs loaded films with different concentrations of low molecular weight chitosan and glycerol were
fabricated and evaluated. Optimized NPs loaded films were assessed for their antimicrobial activity
against the periodontitis patient’s saliva samples.

Results:
The FT-IR spectroscopy and XRD study revealed that there was no interaction between
the drug and all other excipients and the drug remained amorphous form in chitosan film. The
SEM study revealed that the prepared NPs were spherical in shape and uniformly distributed in chitosan film. In vitro drug release study revealed the NPs have a sustained release profile up to 8
days and NPs loaded films have up to 11 days. The conventional marketed mouth wash shows a
low inhibition zone of 5.70 ± 0.043 mm, whereas NPs loaded film shows a higher inhibition zone
of 6.72 ± 0.063 mm against periodontal microorganisms present in the patient’s saliva. The stability study revealed that the optimized NPs loaded film shows no dramatic change in drug release profile and folding endurance after six months.

Conclusion:
This present study highlights the possible usage of cefixime NPs loaded films in enhanced periodontal treatment.