bluuebunny/crossref_metadata_2025_split huggingface dataset:
Intracavitary electrocardiography for femorally inserted central catheter tip location in adult patients
Authors: Duan YingFang , Hu XiaoYan , Zhu YuXin , Zhao Xi , Yin XinXin , Zhang HaiMan , Wang Yu 
Published: December 2021

AbstractBackgroundIn this study, the intracavitary electrocardiogram (ECG) P‐wave and QRS‐wave changes during femorally inserted central catheter (FICC) placement in adults were observed with the aim of reducing malposition occurrence. The observed method provides venous access in patients who have limited upper limb venous catheterization potential and require medium‐term and long‐term infusions.MethodsA retrospective analysis of 34 adult patients who underwent FICC placement was conducted. After body surface measurements were taken, all patients were connected to an ECG during catheter placement, and the P‐wave and QRS‐wave changes were observed. Next, the catheter tip position was confirmed with an abdominal X‐ray, and an analysis of the changes occurring during the procedure was conducted.ResultsIn the 34 patients included in the present study, the catheter tips were located below the diaphragm level in the inferior vena cava. Of the patients, 18 showed negative P waves, biphasic P waves, and positive high‐amplitude P waves with increasing the insertion depth. In 16 patients, no P‐wave characteristic changes were observed during catheterization, and an abdominal X‐ray confirmed that the catheter tip was positioned below the level of the first lumbar vertebra.ConclusionNegative P waves, biphasic P waves, and positive high‐amplitude P waves appeared during FICC placement in adults. Catheter withdrawal until the P wave reverted to normal indicated that a tip position close to the inferior vena cava above the diaphragm level was ideal.
Post‐fire succession of seeding treatments in relation to reference communities in the Great Basin
Authors: Ott Jeffrey E. , Kilkenny Francis F. , Summers Daniel D. , Thompson Tyler W. , Petersen Steven L. 
Published: December 2021

AbstractQuestionsPost‐fire seeding has been widely implemented in the semiarid Great Basin because natural vegetation recovery may be compromised. Non‐native species are often seeded to rapidly establish perennial cover and compete with invasive annuals. We asked whether seeding treatments with different amounts of native and non‐native species followed different successional trajectories and whether they became more similar to reference communities over time. We considered restoration implications of seed mix choices and reference community options involving: (a) local unburned vegetation; and (b) reference states mapped by the USDA Natural Resources Conservation Service (NRCS) based on soil–vegetation associations.LocationTintic Valley, UT, USA.MethodsFour post‐fire seeding treatments differing by seed mix were installed alongside an unseeded control (USC) at two sites. Two seed mixes were comprised of native species and two were predominantly non‐native. Vegetation was monitored 1–3 and 16–18 years after fire and seeding. Reference communities were characterized and compared using hierarchical clustering. Non‐metric multidimensional scaling and permutation tests were used to determine successional trajectories of post‐fire treatments in relation to reference communities.ResultsLocal unburned reference communities had fewer herbaceous perennials and higher woody cover than NRCS reference communities, suggesting departure from conditions expected under minimal post‐settlement disturbance. USCs became more similar to reference communities over time, though less so at a site with abundant invasive annuals. Trajectories of seeded treatments were driven by seed mix species, with native‐only mixes approaching reference communities more closely than mixes with non‐natives.ConclusionsGradual recovery of reference community composition is possible without seeding but the degree and rate of recovery can vary by site. Seeding can accelerate perennial vegetation recovery but may result in alternative successional trajectories, especially if non‐native species are seeded. Carefully selected reference communities can serve as guides for formulating seed mixes when restoration of natural vegetation is desired.
Evaluating sediment recycling through combining inherited petrogenic and acquired sedimentary features of multiple detrital minerals
Authors: Zoleikhaei Yousef , Mulder Jacob A. , Cawood Peter A. 
Published: December 2021

AbstractAn implicit assumption of most sedimentary provenance analyses is a direct link between source and sink. However, recycling of sedimentary detritus from pre‐existing strata interrupts the direct source‐to‐sink link and can result in incorrect interpretations of paleogeography and paleodrainage. Detrital zircon is the favoured proxy of contemporary provenance studies, but its physiochemical resilience makes it particularly prone to recycling. In this study, we integrate geochemical (age, isotope, and trace elements) and grain roundness data of multiple detrital minerals with different physiochemical stabilities (zircon, tourmaline, rutile, and apatite) to evaluate the importance of recycling in an ancient sedimentary basin. We focus on the early Cambrian Lalun Formation of Iran, which forms part of a laterally extensive sandstone‐rich succession deposited along the northern margin of Gondwana. The Lalun Formation preserves a distinct change of compositional maturity between lower arkose and shale units and an upper unit of quartz‐rich sandstone. Detrital zircon, rutile, and apatite data demonstrate that all units of the Lalun Formation share a common source in the Arabian‐Nubian Shield. Whole‐rock geochemical data further indicate that all units have similar chemical alteration indices, suggesting the change in compositional maturity is not a product of differential weathering of the source region. Analysis of grain roundness reveals that detrital zircon, rutile, and tourmaline in the upper quartz‐rich unit are typically more rounded than those in the underlying arkose and shale units. In contrast, detrital apatite grains are nearly all angular in the quartz‐rich unit but mostly rounded in the lower arkose and shale. Together, the detrital mineral provenance, whole‐rock geochemistry, and morphological data are consistent with recycling of the lower arkose and shale units of the Lalun Formation into the uppermost quartz‐rich unit, with the latter also receiving a component of first‐cycle detritus represented by angular detrital apatite. Our findings demonstrate that integrating the features of detrital minerals acquired during a sedimentary cycle (grain rounding and diversity of mineral assemblages) with features inherited from their ultimate source rocks (age, isotopic, and geochemical proxies) can assist in recognising sediment recycling in ancient strata.
Attenuated alpha–gamma coupling in emotional dual pathways with right‐Amygdala predicting ineffective antidepressant response
Authors: Dai Zhongpeng , Pei Cong , Zhang Siqi , Tian Shui , Chen Zhilu , Zhou Hongliang , Lu Qing , Yao Zhijian 
Published: December 2021

AbstractAimsThe diversity of treatment outcomes for major depressive disorder (MDD) remains uncertain in neuropathology. The current study aimed at exploring electrophysiological biomarkers associated with treatment response.MethodsThe present study recruited 130 subjects including 100 MDD patients and 30 healthy controls. All subjects participated in a sad expression recognition task while their magnetoencephalography data were recorded. Patients who had a reduction of at least 50% in disorder severity at endpoint (>2 weeks) were considered as responders. Within‐frequency power and phase‐amplitude coupling were measured for the brain regions involved in the emotional visual information processing pathways.ResultsThe significant alpha–gamma decoupling from the right thalamus to the right amygdala in unconscious processing and from right orbital frontal cortices to the right amygdala in conscious processing was found in non‐responders relative to responders and healthy controls. These kinds of dysregulation could also predict the potential treatment response.ConclusionThe attenuated alpha–gamma coupling in dual pathways indicated increased sensitivity to the negative emotional information and reduced moderated effect of the amygdala, which might cause insensitivity to antidepressant treatment and could be regarded as potential neural mechanisms for treatment response prediction.
Assessment of hand hygiene strategies on skin barrier function during COVID‐19 pandemic: A randomized clinical trial
Authors: Montero‐Vilchez Trinidad , Martinez‐Lopez Antonio , Cuenca‐Barrales Carlos , Quiñones‐Vico Maria I. , Sierra‐Sanchez Alvaro , Molina‐Leyva Alejandro , Gonçalo Margarida , Cambil‐Martin Jacobo , Arias‐Santiago Salvador 
Published: December 2021

AbstractIntroductionCoronavirus disease 2019 (COVID‐19) has increased the frequency of handwashing. There is scarce evidence regarding the impact of different hand hygiene procedures on skin barrier function in clinical practice.ObjectiveTo compare the impact on skin barrier function of different hand hygiene measures in healthcare workers in daily practice.MethodsA randomized controlled clinical trial was conducted. Participants were randomized to sanitize their hands with water and soap, alcohol‐based hand sanitizers (ABHSs), or disinfectant wipes during their 8‐hour working shift. Epidermal barrier functional parameters, such as transepidermal water loss (TEWL), and the microbial load were assessed before and immediately after the working day. Tolerance and acceptability of each product were recorded after work.ResultsSixty‐two participants were included and 20, 21, and 21 were randomized to use water and soap, ABHS, and disinfectant wipes, respectively. After the 8‐hour shift, TEWL increase was higher with disinfectant wipes than with soaps or ABHS (+5.45 vs +3.87 vs −1.46 g h−1 m−2, respectively; P = .023). Bacteria and fungi colony‐forming unit (CFU) count reductions were lower for the water and soap group than for ABHS and disinfectant wipes. Disinfectant wipes were considered more difficult to use (P = .013) compared with water and soap and ABHS.ConclusionDaily hand hygiene with ABHS showed the lowest rates of skin barrier disruption and the highest reduction of CFU.
Substrate stiffness regulates the differentiation profile and functions of osteoclasts via cytoskeletal arrangement
Authors: Wang Qingxuan , Xie Jing , Zhou Chenchen , Lai Wenli 
Published: December 2021

AbstractObjectivesAging and common diseases alter the stiffness of bone tissue, causing changes to the microenvironment of the mechanosensitive bone cells. Osteoclasts, the sole bone‐resorbing cells, play a vital role in bone remodeling. This study was performed to elucidate the mechanism through which osteoclasts sense and react to substrate stiffness signals.Materials and methodsWe fabricated polydimethylsiloxane (PDMS) substrates of different stiffness degrees for osteoclast formation progressed from osteoclast precursors including bone marrow‐derived macrophages (BMMs) and RAW264.7 monocytes. Osteoclast differentiation in response to the stiffness signals was determined by examining the cell morphology, fusion/fission activities, transcriptional profile, and resorption function. Cytoskeletal changes and mechanosensitive adhesion molecules were also assessed.ResultsStiffer PDMS substrates accelerated osteoclast differentiation, firstly observed by variations in their morphology and fusion/fission activities. Upregulation of canonical osteoclast markers (Nfatc1, Acp5, Ctsk, Camk2a, Mmp9, Rela, and Traf6) and the fusion master regulator DC‐stamp were detected on stiffer substrates, with similar increases in their bone resorption functions. Additionally, the activation of cytoskeleton‐associated adhesion molecules, including fibronectin and integrin αvβ3, followed by biochemical signaling cascades of paxillin, FAK, PKC, and RhoA, was detected on the stiffer substrates.ConclusionsThis is the first study to provide evidence proving that extracellular substrate stiffness is a strong determinant of osteoclast differentiation and functions. Higher stiffness upregulated the differentiation profile and activity of osteoclasts, revealing the mechanical regulation of osteoclast activity in bone homeostasis and diseases.
LncRNA‐AK137033 inhibits the osteogenic potential of adipose‐derived stem cells in diabetic osteoporosis by regulating Wnt signaling pathway via DNA methylation
Authors: Peng Shuanglin , Gao Yujin , Shi Sirong , Zhao Dan , Cao Huayue , Fu Ting , Cai Xiaoxiao , Xiao Jingang 
Published: December 2021

AbstractObjectivesBone tissue engineering based on adipose‐derived stem cells (ASCs) is expected to become a new treatment for diabetic osteoporosis (DOP) patients with bone defects. However, compared with control ASCs (CON‐ASCs), osteogenic potential of DOP‐ASCs is decreased, which increased the difficulty of bone reconstruction in DOP patients. Moreover, the cause of the poor osteogenesis of ASCs in a hyperglycemic microenvironment has not been elucidated. Therefore, this study explored the molecular mechanism of the decline in the osteogenic potential of DOP‐ASCs from the perspective of epigenetics to provide a possible therapeutic target for bone repair in DOP patients with bone defects.Materials and methodsAn animal model of DOP was established in mice. CON‐ASCs and DOP‐ASCs were isolated from CON and DOP mice, respectively. AK137033 small interfering RNA (SiRNA) and an AK137033 overexpression plasmid were used to regulate the expression of AK137033 in CON‐ASCs and DOP‐ASCs in vitro. Lentiviruses that carried shRNA‐AK137033 or AK137033 cDNA were used to knockdown or overexpress AK137033, respectively, in CON‐ASCs and DOP‐ASCs in vivo. Hematoxylin and eosin (H&E), Masson's, alizarin red, and alkaline phosphatase (ALP) staining, micro‐computed tomography (Micro‐CT), flow cytometry, qPCR, western blotting, immunofluorescence, and bisulfite‐specific PCR (BSP) were used to analyze the functional changes of ASCs.ResultsThe DOP mouse model was established successfully. Compared with CON‐ASCs, AK137033 expression, the DNA methylation level of the sFrp2 promoter region, Wnt signaling pathway markers, and the osteogenic differentiation potential were decreased in DOP‐ASCs. In vitro experiments showed that AK137033 silencing inhibited the Wnt signaling pathway and osteogenic ability of CON‐ASCs by reducing the DNA methylation level in the sFrp2 promoter region. Additionally, overexpression of AK137033 in DOP‐ASCs rescued these changes caused by DOP. Moreover, the same results were obtained in vivo.ConclusionsLncRNA‐AK137033 inhibits the osteogenic potential of DOP‐ASCs by regulating the Wnt signaling pathway via modulating the DNA methylation level in the sFrp2 promoter region. This study provides an important reference to find new targets for the treatment of bone defects in DOP patients.
Emergency conservation interventions during times of crisis: A case study for a threatened bird species in the Australian Black Summer bushfires
Authors: Selwood Katherine E. , Antos Mark , Bramwell Mick , Lee Adam , Lynch Michael , Magrath Michael J. L. , Maute Kimberly , Melvin Fiona , Mott Rowan , Perri Marc , Whiteford Craig , Clarke Rohan H. 
Published: December 2021

AbstractEmergency conservation interventions will be increasingly necessary to prevent extinctions or severe population bottlenecks as extreme events become more frequent. We detail the emergency extraction of the endangered Eastern Bristlebird (Dasyornis brachpterus) during the unprecedented 2019–2020 Australian Black Summer bushfires, an intervention that led to the rapid establishment of a temporary ex situ insurance population sourced from an area under immediate threat from bushfire (Croajingolong National Park, Victoria). The intervention was triggered, coordinated, and implemented within a 4‐week period, with re‐release to the wild within 2 months. We present this case study within a framework for emergency conservation interventions, based on the emergency management phases of preparation, response, and recovery, with the addition of an evaluation phase. The preparation phase involved compiling existing knowledge and capacity to facilitate the operation. The response phase consisted of (a) initiation and planning of the intervention (coordination) and (b) implementation, that is, the translocation of 15 birds from an area under threat of bushfire to a captive institution (>500 km). The recovery phase saw the insurance population re‐released to unburnt habitat after the bushfire had ceased. The evaluation phase incorporated lessons learnt from the other three phases as part of an adaptive management approach. We reflect on the Eastern Bristlebird emergency conservation intervention to explore how we can better prepare for, respond to, and recover from the large range of emergencies faced by biodiversity around the world.
Chasing the storm: Recruiting non‐hospitalized patients for a multi‐site randomized controlled trial in the United States during the COVID‐19 pandemic
Authors: Hu Kelly , Tardif Jean‐Claude , Huber Melanie , Daly Maria , Langford Aisha T. , Kirby Ruth , Rosenberg Yves , Hochman Judith , Joshi Avni , Bassevitch Zohar , Pillinger Michael H. , Shah Binita 
Published: December 2021

AbstractRandomized controlled trials (RCTs) remain the gold standard to evaluate clinical interventions, producing the highest level of evidence while minimizing potential bias. Inadequate recruitment is a commonly encountered problem that undermines the completion and generalizability of RCTs—and is even more challenging when enrolling amidst a pandemic. Here, we reflect on our experiences with virtual recruitment of non‐hospitalized patients in the United States for ColCorona, an international, multicenter, randomized, placebo‐controlled coronavirus disease 2019 (COVID‐19) drug trial. Recruitment challenges during a pandemic include constraints created by shelter‐in‐place policies and targeting enrollment according to national and local fluctuations in infection rate. Presenting a study to potential participants who are sick with COVID‐19 and may be frightened, overwhelmed, or mistrusting of clinical research remains a challenge. Strategies previously reported to improve recruitment include transparency, patient and site education, financial incentives, and person‐to‐person outreach. Active measures taken during ColCorona to optimize United States recruitment involved rapid expansion of sites, adjustment of recruitment scripts, assessing telephone calls versus text messages for initial contact with participants, institutional review board‐approved financial compensation, creating an infrastructure to systematically identify potentially eligible patients, partnering with testing sites, appealing to both self‐interest and altruism, and large‐scale media efforts with varying degrees of success.
Detecting the phylogenetic signal of glacial refugia in a bryodiversity hotspot outside the tropics
Authors: Wu Ernest T. Y. , Liu Yang , Jennings Linda , Dong Shanshan , Davies T. Jonathan 
Published: December 2021

AbstractIntroductionGlacial refugia have likely been important in shaping diversity gradients outside the tropics. Many taxa that have high extratropical diversity in the present day, such as mosses, may have persisted in glacial refugia. However, the biogeographical histories of most species within refugia remain unclear.LocationHaida Gwaii archipelago, north‐west coast of British Columbia, Canada.TaxaBryophyta (mosses).MethodsWe reconstructed the regional phylogeny of the mosses of Haida Gwaii, a putative glacial refugium and ‘hotspot’ of moss diversity, and used phylogenetic comparative methods to examine the macroecological imprint of glacial refugia on the geographic range structure and phylogenetic attributes of present‐day moss assemblages.ResultsWe found that many mosses have widespread, but disjunct, distributions, with few close relatives on the islands. We suggest that these features reflect the imprint of glacial history, whereby species within refugia represent isolated populations of previously more widespread species that may have diversified elsewhere. We also observed evidence for phylogenetic overdispersion of species within high‐elevation habitats, which best match the climatic regime of the historical glacial refugium. Our results are consistent with the filtering of evolutionarily distinct glacial relicts within these habitats, which contrasts markedly with the patterns of phylogenetic clustering observed across other non‐refugial habitat types.Main conclusionsThe islands of Haida Gwaii represent an extratropical hotspot of bryophyte diversity. Our study illustrates how the present‐day phylogenetic structure of mosses on Haida Gwaii may have been shaped by glacial history, and highlights the importance of glacial refugia in maintaining extratropical moss diversity.
Barriers to and enablers of type 2 diabetes screening among women with prior gestational diabetes: A systematic review update and qualitative synthesis applying the Theoretical Domains Framework
Authors: Lake Amelia J. , Neven Adriana C. H. , Williams Amelia , O'Reilly Sharleen L. , Hendrieckx Christel , Morrison Melinda , Dunbar James , Teede Helena , Boyle Jacqueline A. , Speight Jane ,   the ME‐MaGDA study group
Published: December 2021

AbstractAimsWomen with prior gestational diabetes have nearly 10 times the risk of developing type 2 diabetes. Postpartum screening for type 2 diabetes is recommended for early diagnosis and management, yet uptake is low. This work updates a previous systematic review and advances it through the application of the Theoretical Domains Framework (TDF) to synthesise personal‐level factors impacting type 2 diabetes screening and the Capability, Opportunity, Motivation‐Behaviour model (COM‐B), to develop messaging recommendations for use in clinical practice and screening promotion interventions.MethodsWe searched seven academic databases from September 2017 (prior review) to April 2021, reference lists and grey literature. Two reviewers independently screened articles against inclusion criteria (qualitative studies exploring factors impacting postpartum diabetes screening, any language) and extracted data. Using an inductive‐deductive model, we coded determinants to the TDF and mapped onto the COM‐B model.ResultsWe identified 38 eligible papers from 34 studies (N = 1291 participants). Most (71%) reported sample sizes of N ≥ 16. The ratio of barriers to enablers was three to one. Eight key TDF domains were identified. Evidence‐based recommendations include addressing knowledge, risk perception, fear of diabetes diagnosis, low prioritisation of personal health and fatalism. The risk of bias was low and confidence in findings was moderate to high. A limitation was conceptual overlap between TDF domains, which we addressed via the study procedure.ConclusionsThe theoretical categorisation of determinants enables the development of messaging and interventions at the personal level, to promote women's uptake of postpartum type 2 diabetes screening.