bluuebunny/crossref_metadata_2025_split huggingface dataset:
Long non-coding RNA 01126 activates IL-6/JAK2/STAT3 pathway via serving as a sponge of miR-655-3p in periodontitis
Authors: Tang Song , Li Jie , Yuan Yu , Gong Zhoujie , Wang Zhenxiang , Peng Limin , Zhan Yuzhen , Zhang Xiaonan 
Published: September 2021

Abstract
Background

Periodontitis is a serious threat to oral quality of life and overall health. Although our previous studies confirmed that long intergenic non-coding RNA 01126 (LINC01126) is aberrantly expressed in periodontitis tissues, there are few reports on the pathogenesis of LINC01126 in periodontitis. Our study investigated the biological functions of LINC01126 in periodontitis and the potential mechanism.
Results

An inflammatory model of human gingival fibroblasts (HGFs) was successfully established. LINC01126 silencing can alleviate lipopolysaccharide (LPS) induced cell inflammation, reduce cell apoptosis, and promote cell migration. As a "sponge" for miR-655-3p, LINC01126 inhibits its binding to mRNA of IL-6, thereby promoting inflammation progression and JAK2/STAT3 pathway activation. qRT- PCR, WB, and IHC results of clinical tissue samples further confirmed that miR-655-3p expression was down-regulated and IL-6/JAK/STAT3 was abnormally activated in periodontitis tissues.
Conclusions

Our results indicate that LINC01126, as an endogenous competitive RNA (ceRNA) of miR-655-3p, can promote IL-6/JAK3/STAT3 pathway activation, thereby promoting periodontitis pathogenesis. And this is the first study of miR-655-3p in inflammatory periodontal diseases. Our study reveales a new pathogenesis of periodontitis, and provides a new strategy for preventing and treating periodontitis.
Recovery of human type 2 diabetes beta cell function associates with transcriptomic signatures that point to novel therapeutic targets
Authors: Suleiman Mara , Yi Xiaoyan , Bosi Emanuele , Burdet Frederic , De Luca Carmela , Tesi Marta , Guerra Silvia Del , Pocai Alessandro , De Simone Paolo , Boggi Ugo , Kessler Camille , Eizirik Décio , Marchetti Piero , Cnop Miriam , Ibberson Mark , Marselli Lorella 
Published: September 2021

Abstract
Remission of type 2 diabetes (T2D) may occur after very low-calorie diets or bariatric surgery, and is associated with improved pancreatic beta cell function. Here, we evaluated if T2D beta cell dysfunction can be rescued ex-vivo and which are the molecular mechanisms involved.
Islets from 19 T2D donors were studied after isolation (“basal”) and following culture at 5.5 or 11.1 mmol/l glucose (“cultured”). We evaluated glucose-stimulated insulin secretion (GSIS) and transcriptomes by RNA sequencing, correlated insulin secretion changes (“cultured” vs “basal”) to global gene expression, and searched for potential therapeutic gene targets and compounds that mimic gene signatures of recovered beta cell function in T2D islets.
GSIS improved in 12 out of 19 islet preparations from T2D donors after culture at 5.5 mmol/l glucose (insulin stimulation index increased from 1.4±0.1 to 2.3±0.2, p<0.01), mainly due to greater insulin response to high glucose. No improvement was seen in islets cultured at 11.1 mmol/l glucose. Functional improvement was accompanied by changes in expression of 438 genes, many of which involved in functional and inflammatory processes. Of them, 123 were significantly correlated with changes in glucose-stimulated insulin secretion. Drug repurposing and target identification analyses for beta cell functional recovery predicted several chemical (including Src inhibitors and anti-inflammatory drugs) and genetic hits in pathways such as chemokine, MAPK, ERBB signaling, and autophagy.
In conclusion, defective insulin secretion in T2D can be rescued, at least in part, by a “non-diabetic” milieu, demonstrating important T2D beta cell functional plasticity. This recovery associates with specific transcriptomic traits, pointing to known as well as novel therapeutic targets to induce T2D remission.
EATING HABITS OF OVERWEIGHT CHILDREN AT SEKOLAH DASAR BUDYA WACANA, YOGYAKARTA
Authors: Prasetya Raymond Dwi , Samodra Yoseph Leonardo , Kuntjoro Istianto 
Published: September 2021

Background: Childhood obesity is related to chronic diseases in the future, which could be prevented through changes in eating habit. Strategy in eating behaviour needs an in-depth understanding on contributing factors to unhealthy eating habits in school-age children. Meanwhile, evidence on those factors, especially related increased calorie intake, is still scarce in Indonesia.
Objective: This study is aimed to explore the eating habit of primary school students with obesity in a private school in Yogyakarta.
Method: A qualitative interview involving parents of obese children was performed at Sekolah Dasar Budya Wacana in January 2020. The transcripts were analysed using a thematic approach to acquire major themes related to the cause of unhealthy eating habits.
Results: From 11 parents, there are two major themes developed, which are unhealthy eating habits and parental influences. There are two unhealthy eating habits discovered in this study, which are irregular mealtime and unhealthy food choices. Parental influence which commonly found in children with obesity is permissive parenting style.
Conclusion: This study found similar result with previous studies on unhealthy food choices. However, irregular mealtime in this study is not consistent with other literatures which shows a lack of association with childhood obesity, hence need further research to explore the relationship. Permissive parenting style, which commonly found in younger population also play an important role in children unhealthy food choice. This study revealed that health behaviour change strategy in obese children should consider focussing on healthy food preferences with support from parents and family.
THE POTENTIAL OF RETICULOCYTE HAEMOGLOBIN EQUIVALENT (RET-HE) IN IRON DEFICIENCY ANAEMIA SCREENING AT BETHESDA HOSPITAL, YOGYAKARTA
Authors: Fenty Fenty fenty 
Published: September 2021

Background: Traditionally, iron deficiency anaemia (IDA) is diagnosed with iron and ferritin status. Ret-He measurement is a promising parameter to detect iron depletion in earlier stages. Previous studies recommended Ret-He examination as IDA screening.
Objective: Compare the Ret-He level and iron status of patients with and without IDA at Bethesda Hospital, Yogyakarta.
Method: This is a cross-sectional study using laboratory record of haematological examination at Bethesda Hospital from march to August 2019. Erythrocyte indices, iron status and Ret-He measurement was further examined between IDA and non-IDA group by ANOVA, t-test or non-parametric tests.
Results: There were 105 samples, where 22 (20.95%) had complete result of Ret-He and iron status, and 10 (45.45%) diagnosed with IDA. The Ret-He level in IDA group is significantly lower than non-IDA group (17.53 Â± 2.43 vs 31.50 Â± 4.03, p < 0.001).
Conclusion: Ret-He level is lower in IDA group, consistent with other biochemical parameters (Serum iron, Serum ferritin, TIBC, and TSAT). This finding might lead to future research on the potential of Ret-He for early detection of IDA in high-risk populations.
ASEMPTOMATİK KADINLARDA FARKLI KONTRAKSİYON YÖNTEMLERİNİN PELVİK TABAN KAS FONKSİYONU ÜZERİNE ETKİSİNİN İNCELENMESİ
Authors: ÜNLÜ İdil Esin , ÖZENGİN Nuriye , SERİNDAĞ Sevinç , BAKAR Yeşim , ANKARALI Handan , TOPÇUOĞLU Mehmet Ata 
Published: September 2021

Amaç: Bu çalışma, asemptomatik kadınlarda farklı kontraksiyon yöntemlerinin pelvik taban kas fonksiyonu üzerine etkisini transabdominal ultrasonografi ile değerlendirmeyi amaçladı.
Yöntem: Araştırmaya asemptomatik olduğu Global Pelvik Taban Rahatsızlık Anketi ile belirlenen ve gönüllü olan 120 kadın dahil edildi. Kadınların fiziksel ve sosyodemografik özellikleri kaydedildikten sonra fizyoterapist tarafından 1 saatlik eğitim verildi. Bu eğitimde dört farklı kontraksiyon yöntemi (pelvik taban kas kontraksiyonu, Hollowing in manevrası, korseleme manevrası, anal kontraksiyon) teorik ve pratik olarak öğretildi. Kadınların pelvik taban kas fonksiyonu transabdominal ultrasonografi altında değerlendirildi. Ölçümler her bir kontraksiyon için 3 kez tekrarlandı ve 3 ölçümün ortalaması analize alındı. Kontraksiyonların sıralaması rastgele yöntemle belirlendi.
Sonuçlar: Kadınların kontraksiyon tipine göre pelvik taban kas fonksiyonu Tekrarlı Ölçümlerde Varyans Analizi testiyle karşılaştırıldığında fark olduğu bulundu (p=0,0001). Bu test sonuçlarına göre pelvik taban kas fonksiyonunun korseleme manevrasında en yüksek olduğu, bunu sırasıyla Hollowing in manevrası, pelvik taban kas kontraksiyonu ve anal kontraksiyonun takip ettiği saptandı.
Tartışma: Kadınların pelvik taban kas fonksiyonunun korseleme manevrasında en yüksek, anal kontraksiyon tipinde ise en düşük olduğu bulundu. Pelvik taban kas eğitimi verilirken korseleme manevrasını içeren egzersizlerin öğretilmesinin pelvik taban kas fonksiyonunu artırmada daha etkili olabileceği düşüncesindeyiz.
AŞİL TENDON TAMİRLİ BİREYLERDE CERRAHİ SONRASI UZUN DÖNEM FONKSİYONEL SONUÇLARIN İNCELENMESİ
Authors: TAN Fırat , GUNEY DENİZ Hande , ULUSOY Burak , HARPUT Gülcan , DÖNMEZ Gürhan , DORAL Mahmut 
Published: September 2021

Amaç: Çalışmanın amacı Aşil tendon tamiri (ATT) uygulanan bireylerde cerrahi taraf ile diğer taraf arasında uzun dönem fonksiyonel sonuçları karşılaştırmaktı.
Yöntem: Çalışmaya 20 ATT’li birey (yaş=40,15±7,32 yıl, vücut kitle indeksi=27,17±3,27 kg/m²) dahil edildi. Alt ekstremite fonksiyonel performansını belirlemek için Y denge testi, parmak ucuna yükselme testi ve ayak-ayak bileği araştırması (FAOS) anketi kullanıldı. İstatiksel analiz için cerrahi taraf ile diğer tarafı karşılaştırmada Eşleştirilmiş t testi kullanıldı.
Sonuçlar: ATT’li bireylerin cerrahi sonrası geçen süre ortalama 5,02±3,64 yıl (2,00-13,00) idi. ATT’li bireylerin cerrahi taraf Y denge testi öne (p=0,008), arka içe uzanma mesafeleri (p=0,014) ve parmak ucuna yükselme seviyesi cerrahi tarafta daha az bulundu (p
Ryanodine Receptor as Insecticide Target
Authors: Samurkas Arthur , Yao Li , Hadiatullah Hadiatullah , Ma Ruifang , Xie Yunxuan , Sundarraj Rajamanikandan , Zuilhof Han , Yuchi Zhiguang 
Published: September 2021

:
The ryanodine receptor (RyR) is one of the primary targets of commercial insecticides. The diamide
insecticide family, including flubendiamide, chlorantraniliprole, cyantraniliprole, etc., targets insect RyRs and
can be used to control a wide range of destructive agricultural pests. The diamide insecticides are highly selective
against lepidopteran and coleopteran pests with relatively low toxicity for non-target species, such as mammals,
fishes, and beneficial insects. However, recently mutations identified on insect RyRs have emerged and
caused resistance in several major agricultural pests throughout different continents. This review paper summarizes
the recent findings on the structure and function of insect RyRs as insecticide targets. Specifically, we
examine the structures of RyRs from target and non-target species, which reveals the molecular basis for insecticide
action and selectivity. We also examine the structural and functional changes of RyR caused by the resistance
mutations. Finally, we examine the progress in RyR structure-based insecticide design and discuss how
this might help the development of a new generation of green insecticides.
Surgical Treatment of Pulmonary Embolism and Chronic Thromboembolic
Pulmonary Hypertension
Authors: Papakonstantinou Nikolaos A. , Kampaktsis Polydoros N. , Rorris Filippos-Paschalis , Doulamis Ilias , Tzani Aspasia , Katsaridis Sotirios , Avgerinos Dimitrios 
Published: September 2021

:
Venous thromboembolism clinically presents as deep venous thrombosis or acute pulmonary embolism
and is globally recognized as the third most frequent acute cardiovascular syndrome after myocardial infarction
and stroke. Although pulmonary embolism does not typically cause severe pulmonary hypertension in
the acute setting, thrombus organization and fibrosis can lead to stenosis or obliteration of pulmonary arteries in
a minority of patients, which in turn result in severe pulmonary hypertension and right heart failure. This disease
is labeled chronic thromboembolic pulmonary hypertension and can occur after a single episode or multiple
ones of pulmonary embolism. The cornerstone of pulmonary embolism treatment is medical therapy,
whereas systemic thrombolytic therapy has to be considered for patients with hemodynamic instability. Given
the current acceptable short-term surgical mortality, the potential of first-line surgical embolectomy as an alternative
to medical thrombolysis has gained momentum as far as pulmonary embolism treatment is concerned. In
contrast to pulmonary embolism, bilateral complete pulmonary endarterectomy under short deep hypothermic
circulatory arrest intervals is the treatment of choice against chronic thromboembolic pulmonary hypertension,
given patients’ operability. Pulmonary endarterectomy is suggested in every operable patient when the operation
is offered by an experienced multidisciplinary team, including at least one experienced surgeon. Surgical
embolectomy should also be limited to large institutions since it also requires an experienced heart team. This
review concerns a thorough discussion regarding surgical treatment of pulmonary embolism and chronic thromboembolic
pulmonary hypertension. Eligibility criteria, operation-related complications and postoperative outcomes
are discussed in detail.
Optimal Homotopic Exploration of Features of Cattaneo-Christov Model
in Second Grade Nanofluid Flow via Darcy-Forchheimer Medium Subject
to Viscous Dissipation and Thermal Radiation
Authors: Shafiq Anum , Rasool Ghulam , Chu Yu-Ming , Bhutta Muhammad Shoaib , Ali Amjad 
Published: September 2021

Introduction:
In this article, Optimal Homotopy Analysis Method (oHAM) is used for
the exploration of the features of the Cattaneo-Christov model in viscous and chemically reactive
nanofluid flow through a porous medium with stretching velocity at the solid/sheet surface and free
stream velocity at the free surface.

Methods:
The two important aspects, Brownian motion and Thermophoresis, are considered.
Thermal radiation is also included in the present model. Based on the heat and mass flux, the Cattaneo-
Christov model is implemented on the Temperature and Concentration distributions. The
governing Partial Differential Equations (PDEs) are converted into Ordinary Differential Equations
(ODEs) using similarity transformations. The results are achieved using the optimal homotopy
analysis method (oHAM). The optimal convergence and residual errors have been calculated to
preserve the validity of the model.

Results:
The results are plotted graphically to see the variations in three main profiles. i.e. momentum,
temperature and concentration profile.

Conclusion:
The outcomes indicate that skin friction enhances due to the implementation of the
Darcy medium. It is also noted that the relaxation time parameter results in enhancement of the
temperature distribution. Thermal radiation enhances the temperature distribution and so is the case
with skin friction.
Cepharanthine Regulates Autophagy via Activating the p38 Signaling Pathway in Lung Adenocarcinoma Cells
Authors: Wang Chao , Li Gang , Qiao Kesen , Xu Xiaodan 
Published: September 2021

Background:
Cepharanthine (CEP) is an alkaloid extracted from Stephania cepharantha Hayata. This compound
has been reported as a promising anti-tumor drug, although its potential molecular mechanism is not fully understood.
Here, we studied the anti-tumor effect of CEP on human lung cancer cells and evaluated its molecular
mechanism.

Methods:
The A549 cells were treated with CEP, the cell viability was measured by 3-(4, 5-dimethylthiazolyl-2)-2, 5-
diphenyltetrazolium bromide (MTT) assay, and formation of autophagosome was observed by acridine orange staining
under a fluorescence microscope. The cell migration and invasion were determined by wound healing and transwell
assay. The protein levels of autophagy-associated molecules, light chain 3 (LC3)、p38、and phospho-p38 in A549
cells, were determined by western blot analysis.

Result:
The results showed that CEP inhibited cell proliferation, migration and invasion in A549 cells. Moreover, we
found that CEP resulted in significant increases in levels of the autophagy marker protein LC3 in A549 cells. The
number of intracellular acid dye follicular bright red fluorescence in A549 cells was significantly increased after CEP
treatment. At the molecular levels, CEP markedly increased the phosphorylation of p38 in A549 cells. The knockdown
of p38 expression by siRNA-p38 impaired the autophagy-regulating effect of CEP. Our results indicated that CEPregulated
autophagy was an anti-tumor effect and not a protective response to CEP.

Conclusion:
Taken together, these results demonstrated that CEP regulated autophagy by activating the p38 signaling
pathway, which could be provided a potential application for preventing lung cancer.
In Silico Docking of Novel Phytoalkaloid Camalexin in the Management of Benomyl Induced Parkinson's Disease and its In Vivo Evaluation by Zebrafish Model
Authors: Thamaraikani Tamilanban , Karnam Manasa , Velapandian Chitra 
Published: September 2021

Background:Parkinson’s Disease (PD) exhibits the extrapyramidal symptoms caused due to the dopaminergic neuronal degeneration in the substantia nigra of the brain and depletion of Aldehyde Dehydrogenase (ALDH) enzyme.Objective:This study was designed to enlighten the importance of the Aldehyde dehydrogenase enzyme in protecting the dopamine levels in a living system. Camalexin, a potentially active compound, has been evaluated for its dopamine enhancing and aldehyde dehydrogenase protecting role in pesticide-induced Parkinson’s disease.Methods:AutoDock 4.2 software was employed to perform the docking simulations between the ligand camalexin and standard drugs Alda-1, Ropirinole with three proteins 4WJR, 3INL, 5AER. Consequently, the compound was evaluated for its in vivo neuroprotective role in the zebrafish model by attaining Institutional Animal Ethical Committee permission. The behavioral assessments and catecholamine analysis in zebrafish were performed.Results:The Autodock result shows that the ligand camalexin has a lower binding energy (-3.84) that indicates a higher affinity with the proteins when compared to the standard drug of proteins (-3.42). In the zebrafish model, behavioral studies provided evidence that camalexin helps in the improvement of motor functions and cognition. The catecholamine assay has proved that there is an enhancement in dopamine levels, as well as an improvement in aldehyde dehydrogenase enzyme.Conclusion:The novel compound, camalexin, offers a protective role in Parkinson’s disease model by its interaction with neurochemical proteins and also in alternative in vivo model.
Immunotherapeutics for AD: A Work in Progress
Authors: Sharma Anuja , Anand Jaspreet Singh , Kumar Yatender 
Published: September 2021

:
Alzheimer's Disease (AD), often called the 'Plague of the 21st Century,' is a progressive,
irreversible neurodegenerative disorder that leads to the degeneration and death of neurons. Multiple
factors, such as genetic defects, epigenetic regulations, environmental factors, or cerebrovascular
damage, are a manifestation of the neurodegenerative process that begins to occur decades before
the onset of disease. To date, no treatment or therapeutic strategy has proven to be potent in inhibiting
its progress or reversing the effects of the disease. The ever-increasing numbers and lack
of sufficient therapies that can control or reverse the effects of the disease have propelled research
in the direction of devising efficient therapeutic strategies for AD. This review comprehensively
discusses the active and passive immunotherapies against Amyloid-β and Tau protein, which remain
the popular choice of targets for AD therapeutics. Some of the prospective immunotherapies
against Aβ plaques have failed due to various reasons. Much of the research is focused on targeting
Tau, specifically, targeting the mid-region of extracellular Tau due to their potential to prevent
seeding and hence the spread of neurofibrillary tangles (NFTs). Thus, there is a need to thoroughly
understand the disease onset mechanisms and discover effective therapeutic strategies.
Quality of Life of Older Adults with Primary Open Angle Glaucoma using
Bahasa Malaysia Version of Glaucoma Quality of Life 36 Questionnaire
Authors: Tharmathurai Sangeetha , Huwaina Abdul S. , Azhany Yaakub , Razak Asrenee A. , Che-Hamzah Jemaima , Fazilawati Qamaruddin , Tajudin Liza-Sharmini A. 
Published: September 2021

Background:
Primary Open-Angle Glaucoma (POAG) is an age-related chronic optic
neuropathy causing progressive constriction of visual field, which compromised quality of life
(QoL) of older adults.

Objective :
The study aims to determine the QoL according to the severity of visual field using Bahasa
Malaysia version of the Glaucoma Quality of Life- 36 (Glau-QoL 36) in older adults with
POAG in Malaysia.

Methods:
A cross-sectional study was conducted in two tertiary hospitals in Malaysia: Hospital
Universiti Sains Malaysia, Kelantan, and Hospital Selayang, Selangor. POAG patients who were ≥
60 years old at the time of recruitment had minimal cataract, underwent cataract or trabeculectomy
surgery at least 3 months prior and were on medical and surgical treatment. The severity of POAG
was based on the modified Advanced Glaucoma Intervention Study (AGIS) score on two reliable
reproducible Humphrey visual field SITA program 24-2 analysis. Face to face, one-on-one interview
was conducted using validated Bahasa Malaysia version of GlauQol 36.

Results :
A total of 360 older adults with POAG were recruited. Majority were between ages 60-67
(38.3%) with 64 (17.8%) mild, 93 (25.8%) moderate, 115 (31.9%) severe POAG and 88 (24.4%)
end-stage severity of POAG. The majority of the recruited patients were not working (88.9%) and
live with their families (68.1%). There was a significant association between GlauQoL 36 score of
all domains: daily living, driving, physiological well-being, self-image, anxiety, burden of treatment
and confidence in healthcare with the severity of POAG (p<0.001). Increased severity of
POAG was associated with decreased QoL in all GlauQoL 36 domains except confidence in
healthcare. There was also a significant increase in dependency, with a majority of the end-stage
were living with their families (p<0.001).

Conclusions:
QoL and independency of older adults with POAG decrease with worsening of visual
field defect. Addressing the problem of visual-related activities in older adults with POAG may reduce
their dependency and improve QoL. Happy living is important to lead to healthy living among
older adults with POAG.