bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comparing Efficacy of Punctum-incised Surgery versus Punctum- Sparing Surgery in Primary Canaliculitis Patients
Authors: Shen Zhongcheng , Zhang Qin , Li Fangting , Li Mingwu , Wang Min , Zhao Mingwei 
Published: December 2021

Abstract
Purpose: This study aims to describe the characteristics of canaliculitis patients and compare the operative outcomes between punctum-sparing canaliculotomy and traditional punctum-incised canaliculotomy.Methods: The medical records of 58 patients who were diagnosed with canaliculitis were reviewed from March 1, 2010, to December 31, 2020. The demographic characteristics, symptoms, time to disease onset, location of involvement, operation information, and prognosis were recorded and analyzed.Results: Canaliculitis showed a female (67%) predominance. Epiphora with discharge was the most common symptom which happened in 56 (97%) patients. The recurrence rates of the punctum-incised group and the punctum-sparing group were 5% and 9% respectively, and there was no significant difference between the two groups. (p=0.514)Conclusion: The recurrence rate of the punctum-sparing group showed no significant difference with punctum-incised group. Patients with recurrence finally recovered after multiple treatments. Squeezing by tweezers was a good way to locate the infected lacrimal duct. Punctum-incised surgery can be the first-line therapy for canaliculitis.
Detection of Target Sites Edited in Upland Cotton By The CRISPR/Cas9 System Mediated By Agrobacterium Rhizogenes
Authors: Zhou Lili , Wang Yali , Wang Peilin , Wang Jiamin , Cheng Hongmei 
Published: December 2021

Abstract
Background

CRIPSR/Cas9 gene editing has the ability to effectively modify plant genomes. Multiple target sites usually were designed and the effective target sites were selected for editing. However, upland cotton is allotetraploid and is commonly considered as difficult and inefficient to transform. Therefore, it’s important to quickly identify feasibility of the target site. In this study, we use Agrobacterium rhizogenes K599 strain to infect cotton shoot meristem and induce them to grow hairy roots to detect the feasibility of a selected target designed in GhMYB25-like gene.
Results

We designed a sgRNA within the second exons of GhMYB25-likeA and GhMYB25-likeD and constructed the CRISPR vector. Transient hairy root transformation using A. rhizogenes K599 with four OD600s (0.4, 0.6,0.8, 1.0) was performed in Coker 312 (R15). The results show that A. rhizogenes at OD600 = 0.6–0.8 is the best concentration range for inducing cotton hairy roots. The other three cultivars (TM-1, Lumian 21, Zhongmian 49) were injected using A. rhizogenes K599 with OD600 = 0.6-0.8 and all produced hairy roots. We characterized ten R15 plants with hairy roots and detected different degrees of base deletions and insert at the target site in five R15 plants.
Conclusion

Overall, our data show A. rhizogenes-mediated transient hairy root transformation offers a rapid and efficient method to detect sgRNA feasibility in cotton.
Addition of Ca2+ to Titanium Plates by a Hydrothermal Method and the Effects on Human Gingival Fibroblasts
Authors: Fu Zhendi , Deng Xuehua , Fang Xiaodan 
Published: December 2021

Abstract
Background: Human gingival fibroblasts (hGFs) have key roles in the formation of soft-tissue attachments around dental implants. We added calcium ions (Ca2+) to the surface of titanium plates (TPs) to make it more conducive to the early adhesion and proliferation of hGFs. Methods: Ca2+ was loaded onto the TP surface by a hydrothermal method. The morphology and composition of TP surfaces were determined by scanning electron microscopy and energy-dispersive spectroscopy. Proliferation of hGF-1 cells was measured by the CCK-8 assay. Immunofluorescence staining was done to detect adherent proteins on the TP surface. TPs were divided randomly into two groups: control and Ca.Results: In the Ca group, irregular lamellar crystals were found on the surface of TPs; The percentage of hGF-1 cells adhering to TPs in the Ca group was significantly higher than that in control group (P < 0.01); The fluorescence of integrin-β1 and F-actin in the Ca group was stronger than that in the control group. Conclusions: Our data suggest that Ca2+ can be added to TP surfaces by a hydrothermal method, and can enhance hGF adhesion. This property may be beneficial if Ca2+ is added to titanium surfaces before dental implantation.
FOXF2-Mediated lncRNA LINC02532 Promotes Gastric Cancer Cell Migration and Invasion By Decreasing SOX7 mRNA Stability
Authors: Zhang Cheng , Zhang Chun-Dong , Pei Jun-Peng , Li Yong-Zhi , Yusupu Maimaititusun , Dai Dong-Qiu 
Published: December 2021

Abstract
Background

LncRNAs are known to play a crucial role in the initiation and progression of human diseases, especially cancers. Our previous study demonstrated that dysregulation of LINC02532 facilitated the malignant phenotype of gastric cancer (GC). However, the potential molecular mechanisms regarding the upstream and downstream regulation of LINC02532 in GC progression remain unclear.
Methods

RNA-Seq and clinical data from public databases were used for gene expression and clinical analyses. The subcellular location of LINC02532 was predicted by the bioinformatics tools and further validated by the RNA-Fluorescence in situ hybridization (FISH) assay. The effect of FOXF2/LINC02532/SOX7 axis in GC cell migration and invasion was evaluated using in vitro and in vivo assays. The transcriptional regulation role of FOXF2 and the mRNA stability of SOX7 were explored by dual-luciferase reporter assay and Actinomycin-D drug assay.
Results

We found that high LINC02532 expression was associated with poor prognosis of GC. Furthermore, a Cox regression model indicated that LINC02532 was an independent prognostic factor for GC patients. Using in vitro and in vivo assays, we found that LINC02532 promoted GC cell migration and invasion, as well as tumour growth and metastasis in nude mice. Mechanistically, LINC02532 decreased SOX7 mRNA stability by binding to its 3’UTR, resulting in reduced SOX7 expression. In addition, FOXF2 was identified as a transcriptional factor of LINC02532 and was shown to repress LINC02532 expression by negative transcriptional regulation.
Conclusions

Together, these findings show that LINC02532 promotes GC progression through epithelial–mesenchymal transition (EMT). Cross-talk between the FOXF2/LINC02532/SOX7 axis may provide a novel target for the treatment and prognostic prediction of GC.
PRDX6 Alleviates Lipopolysaccharide-induced Inflammation in Human Gingival Fibroblasts by Regulation of NRF2
Authors: Yang Wen-ying , Meng Xiang , Wang Yue-rong , Wang Qing-qing , He Xin , Sun Xiao-yu , Cheng Nan , Zhang Lei 
Published: December 2021

Abstract
Purpose: Periodontitis is a progressive and inflammatory oral disease and results in the damage of the supporting tissues of teeth. Peroxiredoxin 6 (PRDX6) is an antioxidant enzyme identified as a redox balance regulator. This study aimed to investigate whether PRDX6 could protect human gingival fibroblasts (HGFs) from lipopolysaccharide (LPS) induced inflammation and its mechanisms.Methods: Both inflamed and non-inflamed human gingival tissues were collected to assess the expression of PRDX6 and NRF2 by Immunohistochemistry and Western blotting. Furthermore, HGFs were stimulated with LPS, MJ33 (PRDX6 phospholipase A2 inhibitor), or ML385 (NRF2 inhibitor). The expression levels of inflammatory cytokines were measured by RT-qPCR and ELISA, and reactive oxygen species (ROS) were detected using DCFH-DA.Results: PRDX6 was downregulated in inflamed gingival tissues. In HGFs, LPS induced inflammatory cytokines and ROS was upregulated in PRDX6 knockdown cells. Furthermore, co-treatment with MJ33 alleviated LPS-induced inflammatory cytokines and ROS while inhibiting NRF2 upregulated those in HGFs.Conclusion: Therefore, this study provided a new mechanistic insight that PRDX6, regulated by the NRF2 signaling, alleviates LPS-induced periodontitis in human gingival fibroblasts.
Current and Proposed Treatment Strategies for Siewert Type II Adenocarcinoma of the Esophagogastric Junction
Authors: Narumiya Kosuke , Kudo Kenji , Yagawa Yosuke , Maeda Shinsuke , Toyoshima Yukinori , Ogawa Kyohei , Izumika Sho , Osugi Harushi , Egawa Hiroto 
Published: December 2021

Abstract
BackgroundIncidence of adenocarcinoma of the esophagogastric junction (AEG) is increasing in Japan as well as Western Country. However, there is no consensus on treatment strategy. The purpose of this study was to determine the optimal range of resection and lymph node dissection for Siewert type II AEG and to develop a strategy for treatment that includes adjuvant therapy to improve the survival rate. MethodsWe retrospectively investigate 88 cases of advanced AEG in patients who underwent surgery with lymph node dissection with 52 cases of superficial AEG, 23 of whom underwent endoscopic treatment (endoscopic mucosal resection [EMR] or endoscopic submucosal dissection [ESD]), and 29 of whom underwent surgery with lymph node dissection. ResultsThe optimal lymph nodes to resect for advanced AEG were in the inferior mediastinum (No. 110), in the lesser curvature (Nos. 1, 3, 7), No. 2, and No 11. According to area of actual lymph node metastasis, lymphadenectomy of lymph nodes 1, 2, 3, 7, and 11 was sufficient to improve survival of patients with superficial AEG. If esophageal involvement was >40 mm, we performed esophagectomy through right thoracotomy. The 5-year overall survival rates were 88% for patients treated with ESD, 78% for those with superficial AEG who under-went surgery, and 24% for those with advanced AEG (p = 0.011). Despite of lymph node dissection, twenty-five patients experienced lymph node metastasis after operation in advanced AEG and there were many disseminations in advanced AEG. There were no differences in survival between patients who received postoperative adjuvant therapy with S-1 for advanced AEG and those who received surgery alone (p = 0.5192).Conclusion Although surgical procedures of superficial and locally advanced AEG are standardized, the role of adjuvant therapy for AEG is still controversial. We recommend nab-paclitaxel plus radiotherapy for advanced AEG as neoadjuvant therapy.
Bioaugmentation of Corn Stalks Saccharification with Aspergillus Fumigatus Under Low/High Solid Loading Culture
Authors: Song Zhiwei , Wen Xuechen , Sheng Tao 
Published: December 2021

Abstract
Decomposed the dense structure of lignocellulosic feedstocks and hydrolysis lignocellulose into monosaccharide were essential prerequisite for bio-energy production at this level. In this study, a cellulosic fungi Aspergillus fumigatus CLL was conducted to pretreated the corn stalks under high/low solid loading culture to enhanced the cellulase saccharification performance. The results indicated that A. fumigatus CLL decomposed the corn stalks effectively under high/low solid loading culture, what’s more, A. fumigatus CLL completed the T. reesei cellulase system and promoted the corn stalks saccharification performance. 25.2% lignin was degraded after A. fumigatus CLL treated just for two day under low solid loading culture with holocellulose loss less than 10%. Meanwhile, the β-glucosidase of A. fumigatus CLL complemented the incomplete cellulase system of T. reesei, the maximum saccharification ratio of sample saccharified by T. reesei cellulase combined A. fumigatus CLL was comparable with the sample saccharified by commercial cellulase. Compared with raw corn stalks, the saccharification ratio of pretreated sample increased 3.1-3.4 fold. These results demonstrated that A. fumigatus CLL can be used for pretreatment of lignocellulosic materials to enhanced the saccharification performance.
Analysis of the Development Trend of Clinical Research Nurses in China Based On CiteSpace
Authors: Wu Ning , Li Mingzi 
Published: December 2021

Abstract
Objective

To reveal the development characteristics and trends of clinical research nurses in China and provide a reference for the training and employment of nursing talents.
Methods

Literature about clinical research nurses published from the year of database construction to 2020 were searched through the CNKI, Wanfang, Chinese Biomedical Literature (CBM) and Weipu (VIP) databases, and CiteSpace software was used to conduct a multidimensional analysis of the included literature.
Results

A total of 3,735 pieces of literature were retrieved, and after deduplication and screening, 199 pieces of literature were finally retained for this study. The practice and exploration of CRNs were regionalised, with varying degrees of development, and CRNs were at the forefront of development in oncology specialties.
Conclusion

It is important to continue to expand the breadth and depth of researchto promote the continuous development of China’s medical and health care to align with international standards.
The Homeostasis of p62/SQSTM1 Short Isoform Regulates Selective Autophagy
Authors: Wang Chengcheng , Peng Hong , Wang Zi , Yang Jiao , Li Chuanyin , Hu Ronggui , Geng Wujun 
Published: December 2021

Abstract
Autophagy, a highly conserved lysosomal degradation pathway, has been shown to play a pivotal role in many physiological and pathological processes. Sequestosome 1 (SQSTM1/p62) which serves as autophagy receptor is a multifunctional protein involved in signal transduction, protein degradation and cell transformation. Human SQSTM1 has two isoforms, p62L and p62S, which are derived from alternative splicing at the 5’donor sites. However, few studies focus on p62S, and its function needs to be further explored. Here we found that p62S, but not p62L is mainly degrades by ubiquitin-proteasome signaling pathway. E3 ligase TRIM72 was identified as an interacting partner for p62S, and promotes the ubiquitination and degradation of p62S. Furthermore, we demonstrate that p62S competes with the autophagy receptor p62L which binds to ubiquitinated autophagy cargoes, thus playing a dominant negative role in autophagy regulation, while this inhibitory effect could be attenuated by TRIM72-dependent ubiquitination of p62S. Delineation of the mechanism and regulatory roles of p62S sheds a new light on the proposed pathological implications of p62 in cell physiology. TRIM72 and p62S are promising therapeutic targets for autophagy-related diseases.
The Heat Is on: a Simple Method to Increase Genome Editing Efficiency in Plants
Authors: Blomme Jonas , Develtere Ward , Köse Ayse , Ribera Júlia Arraiza , Brugmans Christophe , Jaraba-Wallace Jessica , Decaestecker Ward , Rombaut Debbie , Baekelandt Alexandra , Fernández Alvaro Daniel Fernández , Breusegem Frank , Inzé Dirk , Jacobs Thomas 
Published: December 2021

Abstract
Background: Precision genome mutagenesis using CRISPR/Cas has become the standard method to generate mutant plant lines. Several improvements have been made to increase mutagenesis efficiency, either through vector optimisation or the application of heat stress. Results: Here, we present a simplified heat stress assay that can be completed in six days using commonly-available laboratory equipment. We show that three heat shocks (3xHS) efficiently increases indel efficiency of LbCas12a and Cas9, irrespective of the target sequence or the promoter used to express the nuclease. The generated indels are primarily somatic, but for three out of five targets we demonstrate that up to 25% more biallelic mutations are transmitted to the progeny when heat is applied compared to non-heat controls. We also applied our heat treatment to lines containing CRISPR base editors and observed a 22-27% increase in the percentage of C-to-T base editing. Furthermore, we test the effect of 3xHS on generating large deletions and a homologous recombination reporter. Interestingly, we observed no positive effect of 3xHS treatment on either approach using our conditions.Conclusions: Together, our experiments show that heat treatment is consistently effective at increasing the number of somatic mutations using many CRISPR approaches in plants and in some cases can increase the recovery of mutant progeny.
New Technique for Detecting Cracked Teeth and Evaluating the Crack Depth by Contrast-enhanced Cone Beam Computed Tomography: an in Vitro Study
Authors: Zhou Jie , Fu Jieni , Xiao Mo , Lv Yangyang , Wu Fei , Qiao Feng , Fu Tiantian , Sun Cuicui , Li Peng , Wu Ligeng 
Published: December 2021

Abstract
Background: Develop a new technique based on contrast-enhanced cone beam computed tomography (CBCT) to improve the detection of cracked teeth and the accuracy of crack depth evaluation in vitro. Methods: We developed an in vitro artificial simulation model of cracked teeth. Pre-experimental CBCT (pre-CBCT), and micro-computed tomography (micro-CT) were first performed for all cracked teeth (n = 31). Contrast-enhanced CBCT was then performed by infiltrating the crack with ioversol under vacuum conditions. The results of pre-CBCT, micro-CT, and contrast-enhanced CBCT were recorded. SPSS v.26.0 software (IBM Corp, Somers, NY) and R software , version 3.6.0 (R Foundation for Statistical Computing; http://www.r-project.org/) and RStudio 1.1.463 (RStudio, PBC, Boston, MA, US) were used to perform the statistical analysis for the study.Results: The sensitivities of pre-CBCT and contrast-enhanced CBCT were 48.4%, and 67.7%, respectively. ICC value of crack depth as measured by pre-CBCT and contrast-enhanced CBCT was 0.847 (95% confidence interval:0.380-0.960; P < 0.001). The areas under ROC curves (AUC) of pre-CBCT and contrast-enhanced CBCT were different, the AUC of pre-CBCT was 0.958 (P = 0.000, 95% CI :0.843-1.074), and the AUC of enhanced CBCT was 0.979 (P = 0.000, 95% CI :0.921-1.037), and the difference was not statistically significant (Z=-0.707, P = 0.480). The ICC value of crack depth as measured by contrast-enhanced CBCT and micro-CT was 0.753 (95% CI: 0.248-0.911; P < 0.001). Conclusion: Contrast-enhanced CBCT under vacuum conditions with a contrast medium can only significantly improve the cracks detection rate of cracked teeth, but not measure the crack depths accurately.
Evaluation of health-related quality of life in patients with Kashin-Beck disease using a new questionnaire：A cross-sectional study
Authors: Jin Zhankui , Sun Zhengming , Wu Xueyuan , Chen Ming , Yang Bo , Dong Xianghui , Liu Shizhang , Chang Yanhai , Xu Cuixiang , Yi Zhi , Ling Ming 
Published: December 2021

Abstract
Objective: Kashin-Beck disease (KBD) is an endemic deformable bone and joint disease, which seriously affects the quality of life (QOL) of patients. We tried to conduct a cross-sectional study of the QOL of KBD patients by a new KBD quality of life (KBDQOL) questionnaire.Methods: 252 KBD patients came from Northwest China, and 260 healthy people living in the same area as KBD patients served as the control group. KBDQOL questionnaire was used to evaluate the QOL of all objects.Results: The average score of physical functions, activity limitations, support of society, mental health and general health of KBD patients was significantly lower than that in control group. There was no statistical difference in economics between KBD patients and control group. The univariate analysis showed that age, height, weight status, education level and grade of KBD had a statistically significant effect on KBDQOL score. The results of multivariate analysis showed that grade of KBD was the influencing factor of physical function score; sex, age, height, grade of KBD and duration of symptoms were the influencing factors of activity restriction score; grade of KBD was the influencing factor of economic score, age and grade of KBD were influencing factors of general health.Conclusions: The QOL of patients with KBD was significantly lower than that of healthy people living in the same place. The KBDQOL questionnaire may be a promising tool for assessing the QOL of KBD patients.
Detection of Deregulated miRNAs in Childhood Epileptic Encephalopathies
Authors: Ünalp Aycan , Coçkunpınar Ender , Gündüz Kübra , Pekuz Serdar , Baysal Bahar Toklu , Edizer Selvinaz , Hayretdağ Ceyda , Güdeloğlu Elif 
Published: December 2021

Abstract
Aims
The term "epileptic encephalopathy" is used to describe a possible relationship between epilepsy and developmental delay. The pathogenesis of developmental encephalopathies, independent of epilepsy, can be defined by genetic control mechanisms. The aim of this study was to investigate the use of miRNAs as serum biomarkers for the determination and discrimination of epileptic encephalopathies.
Methods
Whole blood samples obtained from 54 individuals in 2 groups designated as epileptic encephalopathy patients group (n=24) and healthy controls (n=30) were included in this study. The expression levels of 10 miRNAs were determined using qRT-PCR. After the determination of expression levels the correlation of upregulated miRNA levels and Ki67 index was calculated using Pearson correlation test.
Results
The comparison of epileptic encephalopathy patients group with healthy controls revealed the upregulation of one miRNAs (hsa-miR-324-5p) and downregulation of three miRNAs (hsa-miR-146a-5p, hsa-miR-138-5p, hsa-miR-187-3p).
Conclusion
It has been determined that miRNAs with altered expression are an important factor in the formation of epileptic seizures and seizure-induced neuronal death. The fact that processes that play a key role in epiloptogenesis are under the control of miRNAs causes miRNAs to become meta-controllers of gene expression in the brain. We thouhgt that further studies are needed to prove that especially, hsa-miR-146a-5p, hsa-miR-138-5p and hsa-miR-187-3p can be used as epileptic encephalopathy biomarkers. Detection of disease-specific miRNAs could contribute to the development of presicion treatments.
Transcriptomic Analyses of Differential Host Responses to Red Rot Pathogen Colletotrichum Falcatum in Sugarcane Through Subtractive Library and NGS Approach
Authors: Sathyabhama M. , Viswanathan Rasappa , Prasanth C.N. , Malathi P. , Sundar A. Ramesh 
Published: December 2021

Abstract
The fungal pathogen Colletotrichum falcatum causes the stalks, the economically important for sugar extraction. Although, disease management is achieved by cultivating resistant cultivars, the complex polyploidy of sugarcane genome complicates understanding the inheritance of disease resistance. Earlier attempts of using resistant and susceptible varieties to understand host-pathogen interaction resulted in cultivar specific expression of genes due to different genomic background of the varieties. To avoid host background variation in the interaction, suppression subtractive hybridization (SSH) based next generation sequencing technology was utilized in the same cv Co 7805 which behaves differently as incompatible and compatible to two different C. falcatum pathotypes. In the incompatible interaction (ICI) with C. falcatum pathotype Cf87012 (Less virulent, LVir) 10,038 contigs were assembled from ~54,699,263 raw reads. In the compatible interaction (CI) to the C. falcatum pathotype Cf94012 (Virulent, Vir) 4022 contigs were assembled from ~52,509,239 raw reads. The transcripts homologous to CEBiP receptor and transcripts involved in the signals ROS, Ca2+, BR, JA and ABA were exhibited in both the responses. Additionally, MAPK, ET, PI signals and JA amino conjugation related transcripts were found only in ICI. Finally, the temporal gene expression of a total number of 16 transcripts was monitored in qRT-PCR. Most of the transcripts exhibited highest induction in ICI in comparison with CI. Further, more than 17 transcripts specific to the pathogen were found only in CI, indicating that the pathogen colonizes the host tissue whereas it failed to to do so in ICI. Overall, this study has identified for the first time, the differential responses of a single sugarcane host to two different C. falcatum pathotypes and PAMP triggered immunity (PTI) is exhibited in both the responses, but the more efficient effector triggered immunity (ETI) was found only in ICI at the molecular level.