bluuebunny/crossref_metadata_2025_split huggingface dataset:
Next Generation Oilfield on-Site Trace Chemicals Analysis by SERS
Authors: Liu Zhengwei , Murugesan Sankaran , Ramachandran Sunder , Jin Peng 
Published: November 2021

Abstract
Accurate and precise monitoring of chemical additives in oilfield brine is an important aspect of oil and gas operations towards corrosion control and flow assurance. Many operators are required to monitor the residual concentrations of chemical additives in production systems at specific locations to monitor and troubleshoot factors affecting chemical deliverability and performance. However, residual measurements are extremely problematic due to many factors, including the surface active nature of the chemicals and high ionic strength of the brine. The error on residual measurements can often be over 100%. Residual measurement typically requires the collection of a water sample, which often needs to be transported to a centralized analytical laboratory. Analytical techniques used to measure residuals are based on several combinations of separation (e.g. chromatography, liquid-liquid extraction, etc.) and detection (e.g. various forms of spectroscopy). However, most of these methods lack portability and require tedious laboratory procedures located off-site. The current paper describes a nanotechnology-enabled Raman spectroscopy method developed and tested for monitoring chemical inhibitor residuals. Development of this technology with handheld instrumentation provides better detection and quantification of chemical additives in the field and reduces time and cost compared to sending samples to off-site laboratories for data collection.
Immune landscape evaluation of NSCLC clinical samples
Authors: Lehtiö Janne , Arslan Taner , Siavelis Ioannis , Pan Yanbo , Socciarelli Fabio , Berkovska Olena , Umer Husen M. , Mermelekas Georgios , Pirmoradian Mohammad , Jönsson Mats , Brunnström Hans , Brustugun Odd Terje , Purohit Krishna Pinganksha , Cunningham Richard , Asl Hassan Foroughi , Isaksson Sofi , Arbajian Elsa , Aine Mattias , Karlsson Anna , Kotevska Marija , Hanson Carsten Gram , Haakensen Vilde Drageset , Helland Åslaug , Tamborero David , Johansson Henrik J. , Branca Rui M. , Planck Maria , Staaf Johan , Orre Lukas M. 
Published: November 2021

Abstract
The associated publication reports proteogenomic analysis of non-small cell lung cancer, where we identified molecular subtypes with distinct immune evasion mechanisms and therapeutic targets, and validated our classification method in separate clinical cohorts. This protocol describes histological, tertiary lymphoid structure (TLS), and immunohistochemical evaluation of clinical samples. Specifically, immunohistochemistry was performed for PD-L1, CD3, and CD8 on tumor microarrays (TMAs) derived from formalin-fixed paraffin embedded (FFPE) samples.
American Robin (Turdus Migratorius) Blood Lead Levels May Reflect Elevated Soil Lead Levels: Further Consequences of the Flint Water Crisis
Authors: Zahor Dorothy Louise , Glynn Kenneth Joseph , Cornelius Jamie M. 
Published: November 2021

Abstract
High levels of pollutants often occur in urban environments and can pose a threat to human residents as well as local wildlife. The Flint, Michigan water crisis was caused by the corrosion of pipe infrastructure, resulting in high levels of lead (Pb) leaching into the drinking water. Irrigation with contaminated water may have introduced lead into the soil causing another source of exposure to humans as well as wildlife. A widespread songbird species, the American robin (Turdus migratorius), feeds heavily on earthworms and ingests large amounts of soil during foraging. This study investigated the impact of the Flint water crisis on American robin blood lead levels (BLL) during the breeding season in southeast MI by comparing BLL of birds captured at irrigated sites of Flint to those captured at unirrigated sites in Flint, irrigated sites in a nearby city (Ypsilanti) and rural sites. Robins captured at irrigated Flint sites had nearly double BLL compared to unirrigated Flint sites and all other control sites. Body condition declined with increasing BLL at these irrigated sites of Flint, suggesting a measurable fitness impact of lead at these levels. Because BLL in American robins is known to reflect soil lead levels and soil lead is a known driver of BLL in children, robins may act as a bioindicator for urban communities. Further research should determine the efficacy of using robin BLL as a bioindicator of soil lead and how lead might be impacting body condition and other long-term fitness metrics in urban wildlife.
Visible Light Active Nitrogen and Cobalt Co-doped TiO2 Nanoparticles: Synthesis, Characterization and Photocatalytic Activity
Authors: Javed Aroosa , QAMMAR Memoona , Khurram Rooha , Nisa Zaibun , Nasir Habib 
Published: November 2021

Abstract
A facile and room temperature approach to synthesize pure TiO2 and different variants of nirogen and cobalt co-doped TiO2 (CoN-TiO2) catalysts is reported in this study. The successful synthesis and crystalline phase analysis was carried out via X-ray diffraction (XRD) which confirmed anatase phase with tetragonal structure. The spherical morphology, uniform size distribution in the range of 20-40 nm and presence of dopants in final product were validated by scanning electron microscopy (SEM) and Energy dispersive X-ray spectroscopy (EDS). Diffused reflectance spectroscopy (DRS) is deployed for study of optical properties. A reduction in band gap from 3.2 eV for pure TiO2 nanoparticles to 2.34 eV for the 7 wt.%. doped CoN-TiO2 was observed. The photocatlytic activity of pure TiO2, and CoN-TiO2 nanoparticles was studied against methyl orange.The photocatlytic activity of CoN-TiO2 was almost double as compared to undoped TiO2 which proves these catalysts to be very efficient and potential candidate for the wastewater treatment at industrial level.
Computational, Pharmacological and Toxicological Approach of Repurposed Lamotrigine Schiff Base Derivatives for Reduction of Hormone-Positive Breast Tumor
Authors: Najm Saima , Naureen Humaira , Anwar Fareeha , Khan Muhammad Mubbashir , Ali Rabia 
Published: November 2021

Abstract
Background and objectives: Breast cancer presents high morbidity among women with various treatment challenges. This study aims to evaluate the repurposed lamotrigine schiff base metal (LTG-SB-M) coordinates against in-vitro MCF-7 breast cancer cell lines and in-vivo N-methylnitrosourea (NMU)-persuaded toxicity of rats’ mammary gland. Method: In-silico computational analysis and in vitro cytotoxic studies on MCF-7 breast cancer cell lines was executed to build up the assumptions. In-vivo NMU-induced anticancer potential was assessed in forty Wistar rats; assigned into five groups of 8 rats each. Group I served as normal control and received normal saline, Group II received NMU (50 mg/kg), Group III received tamoxifen, whereas; Group IV and V received LTG-SB-M derivative (LAC3, LBC3) at dose of 100 mg/kg body weight, for 15 consecutive days. Intraperitoneal injection of NMU (single dose) was given at the age of 5, 9 and 13 weeks to the rats with the three week interval. For all experimental animals; biochemical markers were assessed. DNA strand breakage alongside the hormonal profile of estrogen and progesterone was also estimated. Results: All tested compounds present significant activity against MCF-7 cell lines in vitro and NMU-induced mammary tumor in vivo. The in vivo results of tested compounds present a significant decrease in weight of organ; with reinstated renal and hepatic enzymes. Histological analysis revealed strong countenance of proteins, estrogen, and progesterone in NMU-treated rats. Conclusion: These results suggest that LTG-SB-M complex can be used as better anticancer agent against breast cancer.
Study on Competency Elements of Excellent Front-Line Health Inspector Based on BEI
Authors: Liu Yan , Zhou Chunhong , Chen Xiaoling , Yan Yuzhong 
Published: November 2021

Abstract
Background: In order to find out the competency elements of excellent front-line health inspector, and to improve the comprehensive quality and law enforcement level, we conducted this study. Methods: From May to September in 2020, seven health inspectors engaged in front-line inspection and law enforcement work in the Pudong New Area Health Inspection agency were interviewed by using the method of key Behavior Event Interview (BEI). The competency differences of front-line personnel with different performance levels were compared. Combined with the results of expert consultation, the prominent competency elements of excellent front-line health inspector were found out. Results: The total 6 prominent competency elements were investigation and evidence collection ability, on-site control ability, legal awareness, career love, communication and coordination ability, teamwork ability. Cohen's Kappa coefficient of the two coders was more than 0.6, which had high consistency. Conclusion: The key Behavior Event Interview (BEI) is scientific and reasonable method to find out prominent competency elements of excellent front-line health inspector, and the prominent competency elements found out will be helpful in the human resources planning and management of health inspection in the future.
Disabling the Circadian Clock in the Yellow Fever Mosquito Aedes Aegypti Reduces Adult Life Span, Attraction to Host Odor and Mating Success
Authors: Shetty Vinaya , Meyers Jacob I , Zhang Ying , Merlin Christine , Slotman Michel A 
Published: November 2021

Abstract
Like other insects, Aedes aegypti displays strong daily patterns in host seeking and mating. Much of these behaviors are believed to be under the control of a circadian clock, an endogenous timekeeping mechanism relying on transcriptional/translational negative feedback loops that drive rhythmic physiology and behavior. To examine the connection between the circadian clock and various Ae. aegypti behaviors, we knocked out the core clock gene cycle using CRISPR/Cas9. We found that the rhythmic pattern and intensity of mRNA expression of seven circadian genes, including AeCyc-/-, were altered across the day/night cycle as well as in constant darkness conditions. We further show that, if expressed, the mutant CYC protein is incapable of forming a dimer with CLK to stimulate per expression and that the endogenous clock is disabled in AeCyc-/- mosquitoes. AeCyc-/- do not display the bimodal locomotor activity pattern of wild type, have a significantly reduced response to host odor, reduced egg hatching rates, delayed embryonic development, and reduced adult survival and mating success. Surprisingly, however, the propensity to blood feed in AeCyc-/- females is significantly higher than in wildtype females. Together with other recent work on the circadian clock control of key aspects of mosquito biology, our data on how cycle KO affects mosquito behavior and fitness provides a basis for further work into the pathways that connect the mosquito endogenous clock to its vector competence.
Inequalities in COVID-19 Research from Sub-Saharan Africa: Differences in the Prestige of Journal Articles with Internal and External Lead Authors, Collaborations and Publishers
Authors: Asubiaro Toluwase Victor 
Published: November 2021

Abstract
Inequality in health research in Sub-Saharan Africa existed before the onset of the COVID-19 pandemic because of the dearth of research resources. The onset of COVID-19 has exacerbated inequality because of the changes in workplace settings. This study presents an insight into the extent of inequalities during the first year of COVID-19 using citation data of Sub-Saharan African countries' COVID-19 research. Citation data of all the 46 Sub-Saharan Africa countries was collected from Scopus, Web of Science and PubMed. After data cleaning and removal of duplicate records, collaboration type and publishers' country information was coded. Author processing charges of the Open Access articles were obtained from publishers' websites. CiteScore data was collected using Publish or Perish software and Scopus CiteScore report. Only 13.4% of the publishers are in Sub-Saharan African countries, and 21.14% of the articles were published in Sub-Saharan African journals. USA (20.92%) and the UK (13.73%), and India (6.21%) house the highest number of publishers. Publishers from the Netherlands (30.72%), the UK (24.23%) and the USA (14.81%) published the highest number of journals. The CiteScore of journals and mega journals published outside Sub-Saharan Africa was five and twenty-five times more prestigious than those published in the region, respectively. More equitable research practices that will recognize local authors from Sub-Saharan Africa as lead authors in studies about or in Sub-Saharan Africa and journals that are published in the region as a choice for important research may reduce the imbalances as observed in this study.
Improving the Potable Water from Tubular Solar Still Using Eggshell Powder as an Natural Energy Storage Medium - An Experimental Approach
Authors: Thakur Amrit Kumar , Sathyamurthy Prof. Dr. Ravishankar 
Published: November 2021

Abstract
It seems like every hour, there is a greater need for fresh water. The demand for fresh water is rapidly growing as a consequence of the expanding population and the increased urbanization of the world's population. The tubular solar still offers much larger evaporative and condensing surface areas than normal single slope solar still. The scope of this study is to improve the performance of tubular solar still by employing eggshells as the bed material, which has good heat absorption properties. Results showed that the influence of eggshell powder as energy storage material in the basin improved the average water temperature by 10.8, 10.9, and 8.73% for the water thickness of 10, 15, and 20 mm respectively. The usage of eggshells as an energy store in the basin results in an increase of about 60.77 % potable water produced. The maximum observed distillate output from the solar still is 0.6 kg for solar stills with eggshell powder as energy storage material and 0.34 kg for solar stills without eggshell powder in the absorber of TSS at peak solar radiation and at the lowest water thickness of 10 mm. The hourly potable water generated from TSS using eggshell as an energy storage material increased by roughly 47% compared to the flat absorber without eggshell powder. TSS with eggshell powder as energy storage has a daily energy efficiency of 79.19, 75.49, and 44.18 % for water thicknesses of 10, 15, and 20 mm in the basin. Tubular solar still using eggshell as energy storage material and tubular solar still without any material produced 3.62 kg and 1.42 kg average yields at a water thickness of 10 mm. Water thickness of 10, 15, and 20 mm has performance improvement ratios of 2.54, 2.51, and 2.18 respectively.
Virus-like particles displaying conserved toxin epitopes stimulate broadly reactive, polyspecific, murine antibody responses capable of snake venom recognition
Authors: Menzies Stefanie K. , Dawson Charlotte A. , Crittenden Edouard , Edge Rebecca , Hall Steven R. , Alsolaiss Jaffer , Wilkinson Mark C. , Casewell Nicholas R. , Harrison Robert A. , Ainsworth Stuart 
Published: November 2021

Abstract
Antivenom is currently the first-choice treatment for snakebite envenoming. However, only a low proportion of antivenom immunoglobulins are specific to venom toxins, resulting in poor dose efficacy and potency. We sought to investigate whether linear venom epitopes displayed on virus like particles can stimulate a robust and focused antibody response capable of recognising venom toxins from diverse medically important species. Bioinformatically-designed epitopes, corresponding to predicted conserved regions of group I phospholipase A2 and three finger toxins, were engineered for display on the surface of hepatitis B core antigen virus like particles and used to immunise female CD1 mice over a 14-weeks. Antibody responses to all venom epitope virus like particles were detectable by ELISA by the end of the immunisation period, although total antibody and epitope specific antibody titres were variable against the different epitope immunogens. Immunoblots using pooled sera demonstrated recognition of various venom components in a diverse panel of six elapid venoms, representing three continents and four genera. Finally, pooled terminal sera was compared to conventional antivenom via quantitative immunoblot, and demonstrated superior recognition of lower-molecular weight elapid venom toxins. This study demonstrates proof-of-principle that virus like particles engineered to display conserved toxin linear epitopes can elicit specific antibody responses in mice which are able to recognise a geographically broad range of elapid venoms.
Self-Training Algorithm Based on Density Peaks Combining Globally Adaptive Multi-Local Noise Filter
Authors: Li Shuaijun , Lu Jia 
Published: November 2021

Abstract
Self-training algorithm can quickly train an supervised classifier through a few labeled samples and lots of unlabeled samples. However, self-training algorithm is often affected by mislabeled samples, and local noise filter is proposed to detect the mislabeled samples. Nevertheless, current local noise filters have the problems: (a) Current local noise filters ignore the spatial distribution of the nearest neighbors in different classes. (b) They can’t perform well when mislabeled samples are located in the overlapping areas of different classes. To solve the above challenges, a new self-training algorithm based on density peaks combining globally adaptive multi-local noise filter (STDP-GAMNF) is proposed. Firstly, the spatial structure of data set is revealed by density peak clustering, and it is used for helping self-training to label unlabeled samples. In the meantime, after each epoch of labeling, GAMLNF can comprehensively judge whether a sample is a mislabeled sample from multiple classes or not, and will reduce the influence of edge samples effectively. The corresponding experimental results conducted on eighteen real-world data sets demonstrate that GAMLNF is not sensitive to the value of the neighbor parameter k, and it can be adaptive to find the appropriate number of neighbors of each class.
Nutrition Behavior and Body Mass Index in Tanzania. Survey from a Cancer Prevention and Awareness Campaign in Northern Tanzania, 2019‪-‬ 2020‬‬‬‬
Authors: Henke Antje , Rosenbaum Susann , Henke Oliver , Serventi Furaha 
Published: November 2021

Abstract
Objective: In Tanzania, cancer is becoming a major public health concern. Risk factors such as poor dietary behavior, high body mass index, physical inactivity, alcohol and tobacco consumption increase the incidence. Limited cancer treatment facilities, prevention programs, and poor knowledge of cancer risk factors and symptoms in the population contribute to late-stage presentation and high mortality rates. The objective of this study is to examine the association of lifestyle factors including body mass index (BMI), physical activity, and dietary behaviors among participants who attended three cancer prevention events in rural and urban areas in Tanzania. Methods: A cross-sectional survey among PrevACamp- attendees in northern Tanzania between August 2019 and February 2020 were chosen. Participants were interviewed using a structured questionnaire on sociodemographic data, medical history, dietary habits, and physical activity, the body mass index was also determined.Results: 235 participants (114 urban/ 121 rural) were included in the survey. Urban residents had higher rates of obesity (p=0.0021) and less physical activity than participants from rural areas (4.63 days [SD=2.03] and 5.50 days [SD=2.00], respectively (p=0.006). Urban dwellers often skip their lunch and prefer to eat a snack. They use salt frequently, consume more processed meat, eat mainly starchy foods, drink more alcohol and sweetened sodas. Conclusion: Rural women more interested in cancer prevention than men. People in rural areas are more physically active and less overweight than those in urban areas. The cause is manifold, yet they hint at a lack of health care for women and a progressing urbanization according to Western patterns. There is an alarming high percentage of overweight among urban dwellers. Although people eat more healthy foods compared to rural populations, they tend to have a high body mass index. Physical inactivity and overweight will be a serious problem in Tanzania in the future, contributing to the risk of cancer.
Evaluation of Emergency Rescue Efficiency in Coal Mines Based on Co-entropy
Authors: Wang Yumeng , Xihua Zhou , Gang Bai , Li Xianlin , Mufeng Xiao , Yao Rongrong 
Published: November 2021

Abstract
By decomposing the coal mine emergency rescue organization and coal mine emergency rescue work, the synergy entropy function is constructed by combining the synergy class with the entropy theory, and the synergy efficiency assessment model of coal mine emergency rescue is established. Analyze the synergistic relationship between departments in the rescue organization and between departments and functional units, construct the synergistic influence matrix, and obtain the synergistic status of the coal mine emergency rescue system by calculating the synergistic entropy, synergistic efficiency and synergistic degree. The quantitative analysis of the emergency rescue system in coal mines is achieved by analyzing the situation of the rescue work in coal mines and evaluating the rescue organizations at each tier based on the established evaluation model.
Complete Mitochondrial Genomes of Three Mangifera Species, Their Genomic Structure And Gene Transfer From Chloroplast Genomes
Authors: Niu Yingfeng , Gao Chengwen , Liu Jin 
Published: November 2021

Abstract
BackgroundAmong the Mangifera species, mango (Mangifera indica) is an important commercial fruit crop. However, very few studies have been conducted on the Mangifera mitochondrial genome. This study reports and compares the newly sequenced mitochondrial genomes of three Mangifera species. Results Mangifera mitochondrial genomes showed partial similarities in the overall size, genomic structure, and gene content. Specifically, the genomes are circular and contain about 63-69 predicted functional genes, including five ribosomal RNA (rRNA) genes and 24-27 transfer RNA (tRNA) genes. The GC contents of the Mangifera mitochondrial genomes are similar, ranging from 44.42–44.66%. Leucine (Leu) and serine (Ser) are the most frequently used, while tryptophan (Trp) and cysteine (Cys) are the least used amino acids among the protein-coding genes in Mangifera mitochondrial genomes. We also identified 7-10 large chloroplast genomic fragments in the mitochondrial genome, ranging from 1407-6142 bp. Additionally, four intact mitochondrial tRNAs genes (tRNA-Cys, tRNA-Trp, tRNA-Pro, and tRNA-Met) and intergenic spacer regions were identified. Phylogenetic analysis based on the common protein-coding genes of most branches provided a high support value. ConclusionsWe sequenced and compared the mitochondrial genomes of three Mangifera species. The results showed that the gene content of Mangifera mitochondrial genomes is similar across various species. Gene transferred from the chloroplast genome to the mitochondrial genome were identified. This study provides valuable information for evolutionary and molecular studies of Mangifera and a basis for further studies on genomic breeding of mango.