bluuebunny/crossref_metadata_2025_split huggingface dataset:
Conspicuity of Malignant Pleural Mesothelioma in Contrast Enhanced MDCT – Arterial Phase or Late Phase?
Authors: Luerken Lukas , Thurn Philipp Laurin , Zeman Florian , Stroszczynski Christian , Hamer Okka Wilkea 
Published: May 2021

Abstract
Background: To compare two different contrast phases intraindividually regarding conspicuity of MPM in chest MDCT. Methods: 28 patients with MPM were included in this retrospective study. For all patients, chest CT in standard arterial phase and abdominal CT in portal venous phase (scan delay ca. 70 s) was performed. First, subjective analysis of tumor conspicuity was done independently by two radiologists. Second, objective analysis was done by measuring Hounsfield units (HU) in tumor lesions and in the surrounding tissue in identical locations in both phases. Differences of absolute HUs in tumor lesions between phases and differences of contrast (HU in lesion – HU in surrounding tissue) between phases were determined. HU measurements were compared using paired t-test for related samples. Potential confounding effects by different technical and epidemiological parameters between phases were evaluated performing a multiple regression analysis.Results: Subjective analysis: In all 28 patients and for both readers conspicuity of MPM was better on late phase compared to arterial phase. Objective analysis: MPM showed a significantly higher absolute HU in late phase (75.4 vs 56.7 HU, p < 0.001). Contrast to surrounding tissue was also significantly higher in late phase (difference of contrast between phases 18.5 HU, SD 10.6 HU, p < 0.001). Multiple regression analysis revealed contrast phase and tube voltage to be the only significant independent predictors for tumor contrast.Conclusions: In contrast enhanced chest-MDCT for MPM late phase scanning seems to provide better conspicuity and higher contrast to surrounding tissue compared to standard arterial phase scans.
Topical Estrogen, Testosterone, and Vaginal Dilator in the Prevention of Vaginal Stenosis after Radiotherapy in Women with Cervical Cancer
Authors: Martins Jumara , Vaz Ana Francisca , Grion Regina Celia , Costa-Paiva Lúcia , Baccaro Luiz 
Published: May 2021

Abstract
Background: we aimed to evaluate the effects of different therapeutic options to prevent the evolution of vaginal stenosis after pelvic radiotherapy in women with cervical cancer. Methods: open-label randomized clinical trial of 195 women, stage I-IIIB, aged 18-75 years, using topical estrogen (66), topical testosterone (34), water-based intimate lubricant gel (66), and vaginal dilators (29) to assess the incidence and severity of vaginal stenosis after radiotherapy at UNICAMP-Brazil, from January/2013 to May/2018. The main outcome measure was vaginal stenosis assessed using the Common Terminology Criteria for Adverse Events (CTCAE) scale and percental changes in vaginal volume. The women were evaluated at four different times: shortly after the end of radiotherapy, and four, eight, and 12 months after the beginning of the intervention. Statistical analysis was carried out using Symmetry test, Kruskal-Wallis test and multiple regression. Results: the mean age of women was 46.78 (±13.01) years, 61,03% were premenopausal and 73,84% had stage IIB-IIIB tumors. The mean reduction in vaginal volume in the total group was 25.47%, with similar worsening in the four treatment groups with no statistical difference throughout the intervention period. There was worsening of vaginal stenosis evaluated by CTCAE scale after 1 year in all groups (p<0.01), except for the users of vaginal dilator (p=0.37). Conclusions: there was a reduction in vaginal volume in all treatment groups analyzed, with no significant difference between them. However, women who used vaginal dilators had a lower frequency and severity of vaginal stenosis assessed by the CTCAE scale after one year of treatment.Trial registration: Brazilian Registry of Clinical Trials, RBR-23w5fv. Registered 10 January 2017 - Retrospectively registered, https://ensaiosclinicos.gov.br/rg/RBR-23w5fv
Adsorption performance of an amine-functionalized MCM–41 mesoporous silica nanoparticle system for ciprofloxacin removal in a batch system
Authors: Al-Musawi Tariq 
Published: May 2021

Abstract
Antibiotic pollutants discharged from pharmaceutical industries are often present in the aquaticenvironment due to ineffective treatment of pharmaceutical wastewater and are hazardous tohuman and aquatic life. Therefore, effective treatment of antibiotic-containing wastewater is ofutmost importance in the field of environmental protection. This study aims to evaluate theadsorption performance of an amine-functionalized MCM–41 mesoporous silica nanoparticlessystem (MCM–41–NH2) as an adsorbent for the removal of ciprofloxacin (CIP) antibiotic fromaqueous solution. Surface and structural characteristics of MCM–41–NH2 were examined usingscanning electron microscopy, X-ray diffraction, Brunauer–Emmett–Teller analysis, Fouriertransform infrared spectroscopy, and point of zero charge analysis. In addition, thermal stabilitywas investigated by thermogravimetric analysis. Via the proposed treatment, 99.25% CIPremoval was achieved under the following conditions: pH = 7; MCM–41–NH2 dose = 0.8 g/L;CIP concentration = 10 mg/L; adsorption time = 120 min; and shaking speed = 200 rpm.Isotherm study showed that the experimental data fitted well with the Langmuir equation.Moreover, the maximum adsorption capacity of MCM–41–NH2 for CIP was 164.3 mg/g.Thermodynamic parameters showed that the adsorption process of CIP on MCM–41–NH2 wasendothermic and spontaneous. Additionally, the increase in solution temperature had a positiveimpact on the removal of CIP. The kinetic data obtained at different CIP concentrations (10, 25,50, and 100 mg/L) were consistent with the pseudo-second-order model. MCM–41–NH2 couldbe recycled eight times in the proposed adsorption process, with a slight loss in its adsorptioncapacity. Compared with other adsorbents, MCM–41–NH2 was more effective for CIP removal.
Age-Related Increase in Caveolin-1 Expression Facilitates Cell-to-Cell Transmission of α-Synuclein in Neurons
Authors: Ha Tae-Young , Choi Yu Ree , Noh Hye Rin , Cha Seon-Heui , Kim Jae-Bong , Park Sang Myun 
Published: May 2021

Abstract
Parkinson's disease (PD) is the second most prevalent neurodegenerative disease, with aging being considered the greatest risk factor for developing PD. Caveolin-1 (Cav-1) is known to participate in the aging process. Recent evidence indicates that prion-like propagation of misfolded α-synuclein (α-syn) released from neurons to neighboring neurons plays an important role in PD progression. In the present study, we demonstrated that cav-1 expression in the brain increased with age, and considerably increased in the brain of A53T α-syn transgenic mice. Cav-1 overexpression facilitated the uptake of α-syn into neurons and formation of additional Lewy body-like inclusion bodies, phosphorylation of cav-1 at tyrosine 14 was found to be crucial for this process. This study demonstrates the relationship between age and α-syn spread and will facilitate our understanding of the molecular mechanism of the cell-to-cell transmission of α-syn.
The Influencing Factors of Self-evaluation in Adolescents: A Structural Equation Modeling
Authors: Zhai Xiaobing , Zhang Yanan , Eshak Ehab S , Jiang Can , Zeng Jing , Yang Mei , Zhou Jia , Peng Yushuang , Di Lihua , Cao Jinhong , Xiang Bing 
Published: May 2021

Abstract
Background
Adolescents’ self-evaluation is an expressive form of their self-concept. Parental bonding, personality traits and psychological status were the three main determining factors of adolescents’ self-evaluation; however, no study has tested the effects of the three variables simultaneously. We aimed to explore these effects by a systematic approach.
Methods
Data came from the Chinese Family Panel Studies (CFPS). The associations of parental bonding (measured by the Parental Bonding Instrument), personality traits (the Responsibility Scale) and psychological status (the Kessler Psychological Distress Scale, K6) with self-evaluation measured in three domains (the Rosenberg Self-Esteem Scale, the Nowicki-Strickland Locus of Control Scale for children and the Self-Discipline Scale) were tested by the structural equation modeling (SEM) analysis.
Results
A total of 892 Chinese adolescents (Average age = 10.72, SD = 0.89) were included. Significant positive correlations were found among self-evaluation, parental bonding, personality traits and psychological status. The overall (direct and indirect) effect of parental bonding on adolescents’ self-evaluation was 0.49 (parental bonding→ self-evaluation, β = 0.28; parental bonding→ personality traits and psychological status→ self-evaluation, β = 0.21). Personality traits and psychological status were directly associated with adolescents’ self-evaluation (personality trait→ self-evaluation, β = 0.78 and K6→ self-evaluation, β = 0.39).
Conclusions
The use of SEM offered a detailed analysis of the correlations between parental bonding, personality traits, and psychological status, and a systematic approach to investigating their direct and indirect effects on adolescents’ self-evaluation. The three factors were directly associated with adolescents’ self-evaluation and parental bonding had an indirect effect on adolescents’ self-evaluation that was mediated by both personality traits and psychological status.
Treatment optimization of patients with genital endometriosis
Authors: Shcherbina Nikolay , Chekhunova Anastasiia 
Published: May 2021

The aim of the research: to optimize the treatment of patients with combined genital pathology, including internal endometriosis (adenomyosis) and inflammatory diseases. Materials and methods: prospective study has been conducted on 160 women with adenomyosis. There were 24 (15 %) patients with the I degree of adenomyosis spreading, 72 (45.0 %) women with the II degree, 33 (20.6 %) patients with the III degree, and 31 (19.4 %) woman with the IV degree of adenomyosis spreading. Microbial flora analysis included bacterioscopic, bacteriological research methods with determination of sensitivity to antibiotics, and PCR method. The concentration of cytokines in the culture medium (supernatant) was determined by the enzyme immunoassay. Results: the obtained data from the study indicate a high percentage of the combination of adenomyosis with chronic inflammatory diseases of the pelvic organs. An immuno-inflammatory reaction preceding adenomyosis is accompanied by the violation of the local cytokine balance. In turn, the increased activity of cytokines and the presence of infectious agents can participate in the relapse of endometriosis. Conclusion: considering the immuno-inflammatory reaction, accompanied by the violation of the local cytokine balance in the development of adenomyosis. The study substantiates the necessity of using antimicrobial therapy in patients with combined genital pathology, including adenomyosis and inflammatory diseases
Results of bicycle exercise stress test in patients with stable coronary heart disease depending on the angiographic signs of atherosclerotic lesions of coronary arteries
Authors: Tseluyko Vira , Pylova Tetiana 
Published: May 2021

The aim. To identify features of exercise response in coronary heart disease (CHD) patients depending on coronary artery condition and to identify factors associated with a positive test in patients with no obstructive coronary artery disease (INOCA).
Materials and methods. The study included 105 patients diagnosed with stable coronary artery disease (CAD) who were hospitalized at the City Clinical Hospital No. 8 of the Kharkiv City Council. The criteria for diagnosis of ischemia with no obstructive coronary artery disease (INOCA) were met by 53 patients who formed group I. Group II included 52 patients who were consistently hospitalized in the period from June to December 2020, and had obstructive CAD for more than 50 % according to their coronary angiography (CAG).
Results. According to the results of bicycle exercise stress test, a positive test was significantly more often registered in group II – n=30 (57.7 %) patients compared to group I – n=19 (35.8 %) patients (p=0.0249). The duration of the test in patients of group I was significantly longer than 420 seconds [290–540], compared with group II – 300.0 [210.0–540.0] (р=0.0352). Also, in patients in group II, the maximum volume of the test performed was probably lower than in group I (p=0.0324). When calculating the double product, it was also found that in group I its value was significantly higher compared to group II (p=0.0292). In group I there was a significantly higher rate of chronotropic reserve (44.0 [26.0–60.0]), compared with group II (p=0.0168). Elevated total cholesterol (above 5 mmol/l) is a statistically significant and independent factor of a positive exercise test in patients with INOCA (OR, 1.98 [0.9992-3.926], p=0.05). A correlation was found between the level of exercise tolerance and smoking in INOCA-patients (r =-0.388975, p=0.010899). Patients who underwent MINOCA also showed reduced tolerance to exercise (r=-0.3104, p=0.042721)
Conclusions. The sensitivity of bicycle exercise stress test in patients with CAD depends on the presence and severity of atherosclerotic lesions of the coronary arteries (63 % in stenotic atherosclerosis, 36 % in no obstructive coronary arteries. It was found that exercise test duration, double product, chronotropic and inotropic reserve of the heart in patients with a positive exercise test with INOCA were significantly higher compared with patients with obstructive CAD. Independent factors associated with a positive exercise test in patients with no obstructive CAD are an increase in total cholesterol (multivariate regression logistic analysis).
Jk and Mi.III phenotype frequencies in North Vietnam
Authors: Huynh Nguyen Thi , Ford Derek S. , Duyen Tran Thi , Huong Mai Thanh 
Published: May 2021

Abstract
One hundred voluntary blood donors in Hanoi were typed for antigens in the MNS, Rh, Kell, Duffy, and Kidd blood group systems. They were also tested for the presence of the Mi.III (GP.Mur) phenotype and Lewis system antigens. The Jk phenotype frequencies were markedly different from those previously reported. The frequency of the Mi.III phenotype was similar to that reported in Chinese and Taiwanese.
Immunohematology 2003; 19:57–58.
Rh antigen and phenotype frequencies and probable genotypes for the four main ethnic groups in Port Harcourt, Nigeria
Authors: Jeremiah Z.A. , Buseri F.I. 
Published: May 2021

Abstract
Rh is the most complex and polymorphic of the RBC group systems and is of major importance in transfusion medicine. Data are not available on the frequency of Rh antigens D, C, E, c, and e in Port Harcourt, Nigeria. Two mL of venous blood was collected into an EDTA tube from each of 400 persons of mixed ethnic groups recruited for the study. The study population comprised 167 Ijaws (41.8%), 141 Ikwerres (35.2%), 50 Ekpeyes (12.5%), and 42 Ogonis (10.5%). The RBCs were phenotyped for D, C, E, c, and e antigens according to standard serologic methods. The most frequently occurring antigen was found to be c (99.8%), followed by e (98.7%), then D (95.0%), E (20.5%), and finally C (17.7%). The antigens occurred independently of the ethnic groups (p > 0.05) except the antithetical antigens Ee, which were found to be statistically significant in the Ijaw ethnic group when subjected to Pearson chisquare test (χ2 = 9.890, p < 0.02). One (0.2%) of the study population was found to be c– while 20 (5.0%) were D–.
Equivalence of spray-dried K2EDTA, spray-dried K3EDTA, and liquid K3EDTA anticoagulated blood samples for routine blood center or transfusion service testing
Authors: Leathem S. , Zantek N.D. , Kemper M. , Korte L. , Langeberg A. , Sandler S.G. 
Published: May 2021

Abstract
We compared the results of routine blood tests for 102 blood donors’ samples and 100 patients’ samples collected in spray-dried K2EDTA, spray-dried K3EDTA, and liquid K3EDTA blood collection tubes to evaluate the impact of changes in formulation of the anticoagulant (K2EDTA vs. K3EDTA), its application (liquid vs. spray- dried), and tube material (glass vs. plastic). Methods for ABO/D testing, antibody screening, and antibody identification included direct hemagglutination/microplate (Olympus® PK 7200) and gel column methods (Ortho ID-Micro Typing System™/Gel Test™). Additional studies on blood donors’ samples included time delayed antigen testing and antibody identification and half-draw/half- evacuated collections. Also, we compared the results of routine ABO/D testing and antibody screening for 50 patients’ samples collected in spray-dried K2EDTA and spray-dried K3EDTA and for an additional 50 patients’ samples collected in spray-dried K2EDTA tubes from two different manufacturers. All patients’ samples were tested in parallel by solid phase/microplate method (Immucor® ABS 2000) and the standard manual tube method. All test results for routine blood bank tests on donors’ and patients’ samples were concordant, demonstrating the equivalence of spray-dried K2EDTA, spray-dried K3EDTA, and liquid K3EDTA blood collection tubes for routine donor center or transfusion service testing.
Autoanti-D in a patient after cladribine treatment for lymphoplasmocytic lymphoma
Authors: Cid J. , Beltran V. , Escoda L. , Elies E. , Martin-Vega C. 
Published: May 2021

Abstract
We report the case of a 62-year-old woman who developed an autoanti-D after cladribine treatment. In May 2000, the patient underwent splenectomy for a stage IV-B lymphoplasmocytic lymphoma. She was transfused with ABO- and Rh(D)-matched blood. A month later, she received chemotherapy with cladribine. In February 2001, blood grouping showed her to be AB, D+ and the direct antiglobulin test was positive for IgG. An autoanti-D was identified in the eluate. Genotypic analysis confirmed the Rh phenotype of the patient as ccDEe. No hemolysis was evident, as judged by the absence of anemia, a bilirubin of 15.7 μmol/L, and lactic dehydrogenase of 412 IU/L. When an anti-D is identified in a D+ blood recipient, a passive transfer of anti-D, and an alloimmunization in a recipient with a weak D phenotype, should be ruled out. Finally, as in our case, an autoantibody is an additional possibility.
Immunohematology 2002;18:16–18.
The investigation of the significance of a positive direct antiglobulin test in blood donors
Authors: Bellia M. , Georgopoulos J. , Tsevrenis V. , Nomikou E. , Vgontza N. , Kontopoulou-Griva I. 
Published: May 2021

Abstract
Sixty-two samples from 62 donors were investigated to determine the significance of warm IgG autoantibodies that were detected using a gel system during compatibility testing. The presence of autoantibodies on the red cells was confirmed by elution studies. Twelve of 23 strongly positive samples, 7 of 19 moderately positive samples, and 6 of 11 weakly positive samples were studied. The remaining nine samples were found positive during crossmatching, then negative when it was repeated. These nine samples were not included in this study. With a tube test, most of the antibodies had titers from 4 to 8. IgG subclass studies showed that 14 of 25 samples with reactive eluates contained IgG1, one contained IgG1+IgG2, one contained IgG1+IgG4, and two contained IgG1+IgG3 weak. The frequency of donors with a positive direct antiglobulin test (DAT) was ~ 1 in 3000 and males were twice as likely to be DAT positive (8 females vs.17 males in this study). None of the donors had hemolysis. Two donors showed low-titer anticardiolipin antibodies. We conclude that DAT-positive donors can be a problem during compatibility testing when sensitive methods are used.
Immunohematology 2002;18:78–81.
Clinically significant autoimmune hemolytic anemia with a negative direct antiglobulin test by routine tube test and positive by column agglutination method
Authors: Lai M. , Rumi C. , D’Onofrio G. , Voso M.T. , Leone G. 
Published: May 2021

Abstract
In two to five percent of cases of autoimmune hemolytic anemia (AIHA), the patient’s RBCs are negative in the direct antiglobulin test (DAT). We describe a patient with AIHA with a negative DAT when tested by the traditional tube test but with a strongly positive one when tested by column agglutination. When the DAT was repeated by tube test using 4°C saline washes, it became positive. This phenomenon has been observed when low-affinity antibodies are involved in AIHA. The patient’s history and serologic findings are detailed in this report.
Immunohematology 2002;18:109–113.
Comparison of three low-ionic diluents for dilution and storage of reagent A1 and B cells for testing in gel technology
Authors: Steiner E. A. , Dake L. 
Published: May 2021

Abstract
Currently, ABO serum grouping performed by gel technology employs a red cell diluent containing EDTA (MTS Diluent 2 Plus™) that does not permit extended storage of the red cell suspensions. A diluent currently used for suspension and long-term storage of reagent red cells for antibody detection and identification (Ortho 0.8% Red Cell Diluent™) was evaluated for use with A1 and B cells. Because this diluent does not contain EDTA, testing was limited to EDTA samples. As a comparison, a Micro Typing Systems (MTS) diluent not containing EDTA (MTS Diluent 2™) was also tested. MTS-suspended red cells were maintained for 24 hours and compared with Ortho-Clinical Diagnostics 0.8% suspended red cells maintained for 7 days. ABO serum grouping was performed on 144 EDTA plasma samples using all three cell suspensions. Acceptable results were noted in all aspects of testing.
Immunohematology 2001;17: 53–56.
Development of anti-Bw6 reactivity in patients receiving r-GCSF: a preliminary report
Authors: Leach M. F. , Aubuchon J. P. 
Published: May 2021

Abstract
Recombinant granulocyte colony-stimulating factor (r-GCSF) is used in autologous bone marrow/peripheral blood progenitor cell transplantation (ABMT/PBPC) to shorten the period of neutropenia. As these patients require platelet transfusions, their sera may be monitored for the presence of platelet/human leukocyte antigen (HLA) antibodies. Sera of some patients on r-GCSF (16 μg/kg/day) became difficult to evaluate in vitro for the presence of HLA and platelet antibodies because of apparently anomalous reactions in solid phase red cell adherence (SPRCA) assays. SPRCA tests were positive only when platelets were adhered to the polystyrene plates in the presence of glucose; when other simple sugars were used, the patients’ sera failed to react. HLA Bw6-positive platelets were more likely than HLA Bw6-negative platelets to be reactive (p < .001). The transfusion of HLA Bw6-positive platelets to patients displaying this in vitro reactivity (positive patients) resulted in a 50 percent lower corrected count increment (CCI) than those given to patients without it (negative patients; p = <.001). When all transfusions were considered, the CCI for those of the positive patients was decreased 73 percent when compared to the control patients (p = .0005). The presence of the antibody also was associated with a twofold increase in the number of platelet transfusions given (p = .0005). ABMT/PBPC patients receiving r-GCSF may develop unexpected reactions on SPRCA antibody screens and poor responses to transfusion of Bw-6-positive platelets.
Immunohematology 2001;17:63–69.