bluuebunny/crossref_metadata_2025_split huggingface dataset:
Examination of gender differences in patients with takotsubo syndrome according to left ventricular biopsy: two case reports
Authors: Murakami Tsutomu , Komiyama Tomoyoshi , Matsumoto Shingo , Kajiwara Hiroshi , Kobayashi Hiroyuki , Ikari Yuji 
Published: May 2021

AbstractBackgroundTakotsubo syndrome is a stress-induced disease that makes up 2–3% of acute coronary syndrome cases. However, its onset mechanism remains unclear. Although females are overwhelmingly affected, males end up having more cardiac complications.Case presentationWe examined the differences in stress responses in the myocardium between sexes in patients with takotsubo syndrome. We biopsied samples from an over 70-year-old Japanese male and an over 80-year-old Japanese female. Tissues from the left ventricle apex in the acute phase and the apical ballooning-type were examined using histopathology and deoxyribonucleic acid (DNA) microarray analysis. Our data showed that left ventricular ejection fractions were 38% and 56%, and peak creatinine kinase concentrations during hospitalization were 629 U/L and 361 U/L, for the male and female patient, respectively. The pulmonary capillary wedge pressure was 26 mmHg and 11 mmHg for the male and female patient, respectively. Negative T did not return to normal in the male subject after 6 months. Histopathology results indicated that contraction band necrosis and lymphocyte infiltration were more common in the male subject.ConclusionsWe noticed that possible differences may exist between male and female patients using pathological examination and some DNA analyses. In particular, it may help treat acute severity in males. We will elucidate the mechanism of takotsubo syndrome development by increasing the number of samples to support the reliability of the data in the future.
Posttraumatic bronchobiliary fistulae due to foreign body remnants after a road traffic injury: a case report
Authors: Seyed-alagheband Seyed-ahmad , Shahmoradi Mohammad-kazem , Shekouhi Ramin 
Published: May 2021

Abstract
Background
Bronchobiliary fistula is an extremely rare disease that involves abnormal communication between a hepatic segment and bronchial tree. It is mostly caused by untreated hydatid cyst, liver abscess, iatrogenic stenosis, and, rarely, trauma.

Case presentation
We experienced an extremely rare case of bronchobiliary fistula after motor vehicle accident. A 15-year-old Persian boy visited our clinic with chief complaints of persistent pleuritic chest pain, productive cough, weight loss, and fever for 2 months. Coronavirus disease 2019 reverse transcription polymerase chain reaction test was negative. Chest X-ray revealed hazy opacification of right lower lobe. Bronchoalveolar lavage for acid-fast bacillus came back negative. Thoracoabdominal computed tomography scan revealed a collection in segment VIII of the liver communicating with another 13 × 5 cm multiloculated collection in the lower lobe of the right lung, with air foci within the collection. Right posterolateral thoracotomy was performed with the impression of bronchobiliary fistula. Drainage of hepatic collection with debridement, diaphragmatic repair, and open decortication of lung followed by resection of the involved segment of the right lung was performed. Histopathologic evaluations revealed abscess formation in pulmonary tissue, and many multinucleated giant cells were seen that appear to be due to foreign body remnants after previous laparotomy surgery. The foreign body seemed to be the remnants of Surgicel absorbable hemostat.

Conclusions
Herein, we report an extremely rare case of a posttraumatic bronchobiliary fistula caused by remnants of Surgicel hemostatic agent. Bronchobiliary fistula is mainly caused by untreated hydatid cyst, liver abscess, iatrogenic stenosis, and, rarely, trauma. Migration and erosion of oxidized regenerated cellulose through the diaphragm seems to be the causative factor of bronchobiliary fistula in this patient.
Enlightening the taxonomy darkness of human gut microbiomes with a cultured biobank
Authors: Liu Chang , Du Meng-Xuan , Abuduaini Rexiding , Yu Hai-Ying , Li Dan-Hua , Wang Yu-Jing , Zhou Nan , Jiang Min-Zhi , Niu Peng-Xia , Han Shan-Shan , Chen Hong-He , Shi Wen-Yu , Wu Linhuan , Xin Yu-Hua , Ma Juncai , Zhou Yuguang , Jiang Cheng-Ying , Liu Hong-Wei , Liu Shuang-Jiang 
Published: May 2021

AbstractBackgroundIn gut microbiome studies, the cultured gut microbial resource plays essential roles, such as helping to unravel gut microbial functions and host-microbe interactions. Although several major studies have been performed to elucidate the cultured human gut microbiota, up to 70% of the Unified Human Gastrointestinal Genome species have not been cultured to date. Large-scale gut microbial isolation and identification as well as availability to the public are imperative for gut microbial studies and further characterizing human gut microbial functions.ResultsIn this study, we constructed a human Gut Microbial Biobank (hGMB; homepage:hgmb.nmdc.cn) through the cultivation of 10,558 isolates from 31 sample mixtures of 239 fresh fecal samples from healthy Chinese volunteers, and deposited 1170 strains representing 400 different species in culture collections of the International Depository Authority for long-term preservation and public access worldwide. Following the rules of the International Code of Nomenclature of Prokaryotes, 102 new species were characterized and denominated, while 28 new genera and 3 new families were proposed. hGMB represented over 80% of the common and dominant human gut microbial genera and species characterized from global human gut 16S rRNA gene amplicon data (n= 11,647) and cultured 24 “most-wanted” and “medium priority” taxa proposed by the Human Microbiome Project. We in total sequenced 115 genomes representing 102 novel taxa and 13 previously known species. Further in silico analysis revealed that the newly sequenced hGMB genomes represented 22 previously uncultured species in the Unified Human Gastrointestinal Genome (UHGG) and contributed 24 representatives of potentially “dark taxa” that had not been discovered by UHGG. The nonredundant gene catalogs generated from the hGMB genomes covered over 50% of the functionally known genes (KEGG orthologs) in the largest global human gut gene catalogs and approximately 10% of the “most wanted” functionally unknown proteins in the FUnkFams database.ConclusionsA publicly accessible human Gut Microbial Biobank (hGMB) was established that contained 1170 strains and represents 400 human gut microbial species. hGMB expands the gut microbial resources and genomic repository by adding 102 novel species, 28 new genera, 3 new families, and 115 new genomes of human gut microbes.
Moving apart together: co-movement of a symbiont community and their ant host, and its importance for community assembly
Authors: Parmentier T. , Claus R. , De Laender F. , Bonte D. 
Published: May 2021

AbstractBackgroundSpecies interactions may affect spatial dynamics when the movement of one species is determined by the presence of another one. The most direct species-dependence of dispersal is vectored, usually cross-kingdom, movement of immobile parasites, diseases or seeds by mobile animals. Joint movements of species should, however, not be vectored by definition, as even mobile species are predicted to move together when they are tightly connected in symbiont communities.MethodsWe studied concerted movements in a diverse and heterogeneous community of arthropods (myrmecophiles) associated with red wood ants. We questioned whether joint-movement strategies eventually determine and speed-up community succession.ResultsWe recorded an astonishingly high number of obligate myrmecophiles outside red wood ant nests. They preferentially co-moved with the host ants as the highest densities were found in locations with the highest density of foraging red wood ants, such as along the network of ant trails. These observations suggest that myrmecophiles resort to the host to move away from the nest, and this to a much higher extent than hitherto anticipated. Interestingly, functional groups of symbionts displayed different dispersal kernels, with predatory myrmecophiles moving more frequently and further from the nest than detritivorous myrmecophiles. We discovered that myrmecophile diversity was lower in newly founded nests than in mature red wood ant nests. Most myrmecophiles, however, were able to colonize new nests fast suggesting that the heterogeneity in mobility does not affect community assembly.ConclusionsWe show that co-movement is not restricted to tight parasitic, or cross-kingdom interactions. Movement in social insect symbiont communities may be heterogeneous and functional group-dependent, but clearly affected by host movement. Ultimately, this co-movement leads to directional movement and allows a fast colonisation of new patches, but not in a predictable way. This study highlights the importance of spatial dynamics of local and regional networks in symbiont metacommunities, of which those of symbionts of social insects are prime examples.
Genome-wide analysis identifies a novel LINC-PINT splice variant associated with vascular amyloid pathology in Alzheimer’s disease
Authors: Reddy Joseph S. , Allen Mariet , Ho Charlotte C. G. , Oatman Stephanie R. , İş Özkan , Quicksall Zachary S. , Wang Xue , Jin Jiangli , Patel Tulsi A. , Carnwath Troy P. , Nguyen Thuy T. , Malphrus Kimberly G. , Lincoln Sarah J. , Carrasquillo Minerva M. , Crook Julia E. , Kanekiyo Takahisa , Murray Melissa E. , Bu Guojun , Dickson Dennis W. , Ertekin-Taner Nilüfer 
Published: May 2021

AbstractCerebral amyloid angiopathy (CAA) contributes to accelerated cognitive decline in Alzheimer’s disease (AD) dementia and is a common finding at autopsy. The APOEε4 allele and male sex have previously been reported to associate with increased CAA in AD. To inform biomarker and therapeutic target discovery, we aimed to identify additional genetic risk factors and biological pathways involved in this vascular component of AD etiology. We present a genome-wide association study of CAA pathology in AD cases and report sex- and APOE-stratified assessment of this phenotype. Genome-wide genotypes were collected from 853 neuropathology-confirmed AD cases scored for CAA across five brain regions, and imputed to the Haplotype Reference Consortium panel. Key variables and genome-wide genotypes were tested for association with CAA in all individuals and in sex and APOEε4 stratified subsets. Pathway enrichment was run for each of the genetic analyses. Implicated loci were further investigated for functional consequences using brain transcriptome data from 1,186 samples representing seven brain regions profiled as part of the AMP-AD consortium. We confirmed association of male sex, AD neuropathology and APOEε4 with increased CAA, and identified a novel locus, LINC-PINT, associated with lower CAA amongst APOEε4-negative individuals (rs10234094-C, beta = −3.70 [95% CI −0.49—−0.24]; p = 1.63E-08). Transcriptome profiling revealed higher LINC-PINT expression levels in AD cases, and association of rs10234094-C with altered LINC-PINT splicing. Pathway analysis indicates variation in genes involved in neuronal health and function are linked to CAA in AD patients. Further studies in additional and diverse cohorts are needed to assess broader translation of our findings.
In vivo 5-ethynyluridine (EU) labelling detects reduced transcription in Purkinje cell degeneration mouse mutants, but can itself induce neurodegeneration
Authors: van’t Sant Lisanne J. , White Joshua J. , Hoeijmakers Jan H. J. , Vermeij Wilbert P. , Jaarsma Dick 
Published: May 2021

AbstractFluorescent staining of newly transcribed RNA via metabolic labelling with 5-ethynyluridine (EU) and click chemistry enables visualisation of changes in transcription, such as in conditions of cellular stress. Here, we tested whether EU labelling can be used to examine transcription in vivo in mouse models of nervous system disorders. We show that injection of EU directly into the cerebellum results in reproducible labelling of newly transcribed RNA in cerebellar neurons and glia, with cell type-specific differences in relative labelling intensities, such as Purkinje cells exhibiting the highest levels. We also observed EU-labelling accumulating into cytoplasmic inclusions, indicating that EU, like other modified uridines, may introduce non-physiological properties in labelled RNAs. Additionally, we found that EU induces Purkinje cell degeneration nine days after EU injection, suggesting that EU incorporation not only results in abnormal RNA transcripts, but also eventually becomes neurotoxic in highly transcriptionally-active neurons. However, short post-injection intervals of EU labelling in both a Purkinje cell-specific DNA repair-deficient mouse model and a mouse model of spinocerebellar ataxia 1 revealed reduced transcription in Purkinje cells compared to controls. We combined EU labelling with immunohistology to correlate altered EU staining with pathological markers, such as genotoxic signalling factors. These data indicate that the EU-labelling method provided here can be used to identify changes in transcription in vivo in nervous system disease models.
An evaluation of space weather conditions for FORMOSAT-3 satellite anomalies
Authors: Shen Han-Wen , Shue Jih-Hong , Dombeck John , Lee Tsung-Ping 
Published: May 2021

AbstractThe variable electromagnetic environment in geospace plays a crucial role in influencing the occurrence probability of satellite anomalies. FORMOSAT-3 (FS3) is a Low-Earth-Orbit (LEO) mission, which consists of six identical microsatellites that orbit in the altitude of 700–800 km and with an inclination of 72°. The dependences of the FS3 satellite anomalies on space weather conditions have not been investigated in the past. With an exception of a small number of extremely high geomagnetic events, we find that the occurrence rate of the FS3 anomalies is negatively correlated with the level of geomagnetic activity. Moreover, the relationship between numbers of anomalies and sunspots is also anti-correlated. A superposed epoch analysis demonstrates that the intensity of galactic cosmic rays (GCR) is relatively high at the times of the anomalies. All these results infer that the FS3 anomalies predominantly occurred under the conditions associated with low solar activity. The possible main cause for the FS3 anomalies is high-energy trapped protons or GCR. In summary, this paper presents a statistical result that a satellite can be prone to suffer an anomaly under low solar or geomagnetic activity.
A multi-enzyme cascade for efficient production of d-p-hydroxyphenylglycine from l-tyrosine
Authors: Tan Xu , Zhang Sheng , Song Wei , Liu Jia , Gao Cong , Chen Xiulai , Liu Liming , Wu Jing 
Published: May 2021

AbstractIn this study, a four-enzyme cascade pathway was developed and reconstructed in vivo for the production of d-p-hydroxyphenylglycine (D-HPG), a valuable intermediate used to produce β-lactam antibiotics and in fine-chemical synthesis, from l-tyrosine. In this pathway, catalytic conversion of the intermediate 4-hydroxyphenylglyoxalate by meso-diaminopimelate dehydrogenase from Corynebacterium glutamicum (CgDAPDH) was identified as the rate-limiting step, followed by application of a mechanism-guided “conformation rotation” strategy to decrease the hydride-transfer distance d(C6HDAP−C4NNADP) and increase CgDAPDH activity. Introduction of the best variant generated by protein engineering (CgDAPDHBC621/D120S/W144S/I169P with 5.32 ± 0.85 U·mg−1 specific activity) into the designed pathway resulted in a D-HPG titer of 42.69 g/L from 50-g/L l-tyrosine in 24 h, with 92.5% conversion, 71.5% isolated yield, and > 99% enantiomeric excess in a 3-L fermenter. This four-enzyme cascade provides an efficient enzymatic approach for the industrial production of D-HPG from cheap amino acids.
Integrated algal and oil palm biorefinery as a model system for bioenergy co-generation with bioproducts and biopharmaceuticals
Authors: Abdullah Mohd Azmuddin , Hussein Hanaa Ali 
Published: May 2021

AbstractBackgroundThere has been a greater call for greener and eco-friendly processes and bioproducts to meet the 2030’s core agenda on 17 global sustainable development goals. The challenge lies in incorporating systems thinking with a comprehensive worldview as a guiding principle to develop the economy, whilst taking cognisance of the need to safeguard the environment, and to embrace the socio-cultural diversity dimension as an equal component. Any discussion on climate change, destruction of eco-system and habitat for wildlife, poverty and starvation, and the spread of infectious diseases, must be addressed together with the emphasis on the development of cleaner energy, air and water, better management of resources and biodiversity, improved agro-practices for food production and distribution, and affordable health care, as the outcomes and key performance indicators to be evaluated. Strict regulation, monitoring and enforcement to minimize emission, pollution and wastage must also be put in place.ConclusionThis review article focuses on the research and development efforts to achieve sustainable bioenergy production, environmental remediation, and transformation of agro-materials into value-added bioproducts through the integrated algal and oil palm biorefinery. Recent development in microalgal research with nanotechnology as anti-cancer and antimicrobial agents and for biopharmaceutical applications are discussed. The life-cycle analysis in the context of palm oil mill processes is evaluated. The way forward from this integrated biorefinery concept is to strive for inclusive development strategies, and to address the immediate and pressing problems facing the Planet and the People, whilst still reaping the Profit.
Is nesting in closed nestboxes advantageous for the European Robin (Erithacus rubecula)?
Authors: Juškaitis Rimvydas 
Published: May 2021

AbstractBackgroundThe European Robin (Erithacus rubecula) is eurytopic in its choice of nest site, which can be either half-open or closed, and situated either on the ground or at a height of several meters. On occasion, robins also nest in closed nestboxes, though generally only solitary such cases are documented, albeit that dozens of such events can be recorded during the course of some long-term studies. However, until now, nobody has summarised the peculiarities of robins nesting in closed nestboxes.MethodsIn the period 1978–2020, wooden tit and starling nestboxes were inspected regularly at five study sites in Lithuania, this totaling more than 18,000 nestbox-seasons. During these inspections, 90 cases of robins nesting in the nestboxes were recorded. Publications on this topic from the entire robin distribution range were reviewed.ResultsRobins prefer to nest in old large-sized fairly shallow nestboxes with wide entrance holes, for example starling nestboxes or tit nestboxes with enlarged entrance holes. Increased numbers of nestboxes being occupied by robins were recorded for 3–8 years in row. In Lithuania, nesting success in nestboxes is not higher than compared with nesting on the ground. Tree climbing mammals, Pine Martens (Martes martes), Hazel Dormice (Muscardinus avellanarius), Edible Dormice (Glis glis) and Forest Dormice (Dryomys nitedula), are the main predators of robin nests in nestboxes.ConclusionsSome geographic variation was found in the occurrence of robins nesting in nestboxes with more such cases recorded in central and southern parts of the range. Possibly robins are more philopatric in these parts of the range, with the same females or their offspring nesting in nestboxes for several years in row. In areas inhabited by dormice, nesting in closed nestboxes is not advantageous for robins.
Enskilment: an Ecological-Anthropological Worldview of Skill, Learning and Education in Sport
Authors: Woods Carl T. , Rudd James , Gray Rob , Davids Keith 
Published: May 2021

AbstractThe aim of this paper is to explore a different, more relational worldview of skill, learning and education in sport. To do this, we turn to the work of social anthropologist, Tim Ingold, leaning on the notion of enskilment, which proposes thatlearningis inseparable fromdoingandplace. From this worldview, what is learned is not an established body of knowledge, transmitted into the mind of a passive recipient from an authorised being, but is a progressively deepening embodied-embedded attentiveness, where an individual learns to self-regulate by becoming more responsive to people and environmental features by ‘looking, listening and feeling’. As we discuss, Ingold’s perspectives on enskilment are rooted in the etymological connotations of education—ex-ducere, which roughly means ‘to lead out’. In applying this notion to sport, we unpack three of its entangled components,taskscapes,guided attention, andwayfinding, detailing the implications of each for the growth ofenskilledsports performers. To promote the translation of these ideas, in addition to encouraging their inquiry beyond the scope of what is discussed here, sporting examples are threaded throughout the paper.
Fintech investments in European banks: a hybrid IT2 fuzzy multidimensional decision-making approach
Authors: Kou Gang , Olgu Akdeniz Özlem , Dinçer Hasan , Yüksel Serhat 
Published: May 2021

AbstractFinancial technology (Fintech) makes a significant contribution to the financial system by reducing costs, providing higher quality services and increasing customer satisfaction. Hence, new studies play an essential role to improve Fintech investments. This study evaluates Fintech-based investments of European banking services with an application of an original methodology that considers interval type-2 (IT2) fuzzy decision-making trial and evaluation laboratory and IT2 fuzzy TOPSIS models. Empirical findings are controlled for consistency by applying the VIKOR method. Moreover, we conduct a sensitivity analysis by considering six distinct cases. This study contributes to the existing literature by identifying the most important Fintech-based investment alternatives to improve the financial performance of European banks. Our empirical findings illustrate that results are coherent, reliable, and identify “competitive advantage” as the most important factor among Fintech-based determinants. Moreover, “payment and money transferring systems” are the most important Fintech-based investment alternatives. It is recommended that, among Fintech-based investments, European banks should mainly focus on payment and money transferring alternatives to attract the attention of customers and satisfy their expectations. This is also believed to have a positive impact on the ease of bank’ receivable collection. Another important point is that Fintech-based investments in money transferring systems could help to decrease costs.
The intermediary players affect the altruism behavior on the bipartite network
Authors: Guo Linghui , Liu Zhongxin , Chen Zengqiang 
Published: May 2021

Abstract
A myriad of participants in the market without direct interactions could act in a coordinated way thanks to the extremely hierarchical and diversified social structures developed spontaneously based on the economic mechanism. This paper investigates the function of the intermediary nodes on the network that may affect the altruistic behavior of the neighbours in the evolutionary games. To achieve this, a pairwise game is proposed on the bipartite graph inspired by the public goods game, where two layers of nodes are defined: the player nodes as the game participants, and the group nodes as the intermediary hubs. Specifically, a tolerance parameter is introduced to modify the behavior of the group nodes, which theoretically raises the surviving threshold of cooperators if sufficiently large. However, a double-edged effect is observed in the experiments where large values of the parameter are applied, which on the one hand helps the cooperators to survive, while it prevents the total elimination of exploiters, indicating the complex relevance between the roles of the intermediary nodes and the system behavior.