bluuebunny/crossref_metadata_2025_split huggingface dataset:
DNM1L-Related Mitochondrial Fission Defects Presenting as Encephalopathy: A Case Report and Literature Review
Authors: Liu Xingmiao , Zhang Zhongbin , Li Dong , Lei Meifang , Li Qing , Liu Xiaojun , Zhang Peiyuan 
Published: July 2021

Background: Mitochondrial dynamics, including mitochondrial fission and fusion, transport and distribution, biogenesis and degradation, are critical to neuronal function. The dynamin-1 like (DNM1L) gene encodes dynamin-related protein 1 (DRP1/DLP1), which is an evolutionarily conserved member of the dynamin family and is responsible for mitochondrial division. DNM1L variants can lead to mitochondrial fission dysfunction and neurological disorders.Methods: We report a case of DNM1L-related mitochondrial disease admitted to Tianjin Children's Hospital. We searched for similar reported cases in the PubMed database using the terms “DNM1L” and “mitochondrial,” reviewed recent literature to summarize the clinical and genetic characteristics, and analyzed genotype-phenotype correlations.Results: The patient presented with psychomotor retardation, motor disturbance (muscle weakness with paroxysmal hypermyotonia), and a de novo variant (c.116G&gt;A, g.22229G&gt;A, p.S39N) in the GTPase domain of DNM1L (reference sequence NM_012062), which has not previously been reported in the literature. This case was combined with an additional 35 cases identified in 20 relevant references in order to analyze a total of 36 patients. The male-to-female ratio was 1:1.06, and the median age of onset was 6 months (range, neonatal period to 9 years). The cardinal symptoms included psychomotor retardation in 77.8% (28/36), limb paralysis in 66.7% (18/27), dystonia in 82.8% (24/29), and epilepsy in 59.4% (19/32). The clinical manifestations of variants in the GTPase domain of DRP1 were milder than those identified in the middle domain.Conclusion: This case report describes a new variant of the DNM1L gene, and summarizes previously reported cases. Furthermore, the clinical phenotype and the genotype of DNM1L gene-associated mitochondrial disease was analyzed to improve the understanding of this disease.
High Prevalence of Helicobacter pylori Infection in Special Needs Schools in Japan
Authors: Kakiuchi Toshihiko , Takamori Ayako , Matsuo Muneaki 
Published: July 2021

Background: Developmental disorders and high Helicobacter pylori (H. pylori) infection rates have been reported. This study aimed to examine the prevalence of H. pylori in a special needs school where all students had developmental disorders in Japan.Methods: In 2017, third-grade junior high school and second- and third-grade high school students attending a special needs school with developmental disorders were enrolled. Participants of Saga Prefecture's H. pylori test and treat project, which comprised third-grade junior high school students not from special needs school, were assigned to the control group.Results: In the control group, H. pylori positive results were 3.18% (228/7,164) students. Similarly, in developmental disorder group, H. pylori positive results were 6.80% (13/191) students. For the developmental disorder and control groups, this present examination sensitivity was 7.03% (13/185), specificity was 96.76% (6,815/7,043), positive predictive value was 5.39% (13/241), negative predictive value was 97.54% (6,815/6,987), Likelihood ratio of a positive result 2.17 and Odds ratio was 2.26 (95% confidence interval: 1.27–4.03, p = 0.005).Conclusion: The prevalence of H. pylori infection was significantly higher in adolescents with developmental disorders than in typically developing adolescents.
Mildly Increased Renin Expression in the Absence of Kidney Injury in the Murine Transverse Aortic Constriction Model
Authors: Wesseling Marian , Mulder Eva , Brans Maike A. D. , Kapteijn Daniek. M. C. , Bulthuis Marian , Pasterkamp Gerard , Verhaar Marianne C. , Danser A. H. Jan , van Goor Harry , Joles Jaap A. , de Jager Saskia C. A. 
Published: July 2021

Cardiorenal syndrome type 2 is characterized by kidney failure as a consequence of heart failure that affects &gt;50% of heart failure patients. Murine transverse aortic constriction (TAC) is a heart failure model, where pressure overload is induced on the heart without any systemic hypertension or its consequences. Whether renal function is altered in this model is debated, and if so, at which time post-TAC renal dysfunction starts to contribute to worsening of cardiac function. We therefore studied the effects of progressive heart failure development on kidney function in the absence of chronically elevated systemic blood pressure and renal perfusion pressure. C57BL/6J mice (N = 129) were exposed to TAC using a minimally invasive technique and followed from 3 to 70 days post-TAC. Cardiac function was determined with 3D ultrasound and showed a gradual decrease in stroke volume over time. Renal renin expression and plasma renin concentration increased with progressive heart failure, suggesting hypoperfusion of the kidney. In addition, plasma urea concentration, a surrogate marker for renal dysfunction, was increased post-TAC. However, no structural abnormalities in the kidney, nor albuminuria were present at any time-point post-TAC. Progressive heart failure is associated with increased renin expression, but only mildly affected renal function without inducing structural injury. In combination, these data suggest that heart failure alone does not contribute to kidney dysfunction in mice.
Alpha-Lipoic Acid Protects Co-Exposure to Lead and Zinc Oxide Nanoparticles Induced Neuro, Immuno and Male Reproductive Toxicity in Rats
Authors: Deore Monika S. , S Keerthana , Naqvi Saba , Kumar Anoop , Flora S. J. S. 
Published: July 2021

We evaluated the neuro-, immuno-, and male reproductive toxicity of zinc oxide nanoparticles (ZnO NPs) alone and in combination with lead acetate. We also studied the therapeutic role of α-lipoic acid postexposure. Lead (10 mg/kg, body weight), ZnO NPs (100 mg/kg, bwt) alone, and their combination were administered orally in Wistar rats for 28 days, followed by the administration of α-lipoic acid (15 mg/kg, bwt) for the next 15 days. Our results demonstrated protective effects of α-lipoic acid on lead and ZnO NP–induced biochemical alterations in neurological, immunological, and male reproductive organs in rats. The altered levels of blood δ-aminolevulinic acid dehydratase (ALAD), immunoglobulins (IgA, IgG, IgM, and IgE), interleukins (IL-1β, IL-4, and IL-6), caspase-3, and tumor necrosis factor (TNF-α) were attenuated by lipoic acid treatment. Lead and ZnO NP–induced oxidative stress was decreased by lipoic acid treatment, while a moderate recovery in the normal histoarchitecture of the brain section (cortex and hippocampus) and testes further confirmed the neuro- and male reproductive toxicity of lead and ZnO NPs. We also observed a significant decrease in the blood metal content in the animals treated with lipoic acid compared to the lead-administered group, indicating the moderate chelating property of lipoic acid. It may thus be concluded that lipoic acid might be a promising protective agent against lead and ZnO NP–induced alterations in the neurological, immunological, and reproductive parameters.
Exploring Phytochemicals of Traditional Medicinal Plants Exhibiting Inhibitory Activity Against Main Protease, Spike Glycoprotein, RNA-dependent RNA Polymerase and Non-Structural Proteins of SARS-CoV-2 Through Virtual Screening
Authors: Nallusamy Saranya , Mannu Jayakanthan , Ravikumar Caroline , Angamuthu Kandavelmani , Nathan Bharathi , Nachimuthu Kumaravadivel , Ramasamy Gnanam , Muthurajan Raveendran , Subbarayalu Mohankumar , Neelakandan Kumar 
Published: July 2021

Severe Acute Respiratory Syndrome Corona Virus 2 (SARS-CoV-2) being a causative agent for global pandemic disease nCOVID’19, has acquired much scientific attention for the development of effective vaccines and drugs. Several attempts have been made to explore repurposing existing drugs known for their anti-viral activities, and test the traditional herbal medicines known for their health benefiting and immune-boosting activity against SARS-CoV-2. In this study, efforts were made to examine the potential of 605 phytochemicals from 37 plant species (of which 14 plants were endemic to India) and 139 antiviral molecules (Pubchem and Drug bank) in inhibiting SARS-CoV-2 multiple protein targets through a virtual screening approach. Results of our experiments revealed that SARS-CoV-2 MProshared significant disimilarities against SARS-CoV MProand MERS-CoV MProindicating the need for discovering novel drugs. This study has screened the phytochemical cyanin (Zingiber officinale) which may exhibit broad-spectrum inhibitory activity against main proteases of SARS-CoV-2, SARS-CoV and MERS-CoV with binding energies of (−) 8.3 kcal/mol (−) 8.2 kcal/mol and (−) 7.7 kcal/mol respectively. Amentoflavone, agathisflavone, catechin-7-o-gallate and chlorogenin were shown to exhibit multi-target inhibitory activity. Further,Mangifera indica, Anacardium occidentale, Vitex negundo, Solanum nigrum, Pedalium murex, Terminalia chebula, Azadirachta indica, Cissus quadrangularis, Clerodendrum serratum and Ocimum basilicumaree reported as potential sources of phytochemicals for combating nCOVID’19. More interestingly, this study has highlighted the anti-viral properties of the traditional herbal formulation “Kabasura kudineer” recommended by AYUSH, a unit of Government of India. Short listed phytochemicals could be used as leads for future drug design and development. Genomic analysis of identified herbal plants will help in unraveling molecular complexity of therapeutic and anti-viral properties which proffer lot of chance in the pharmaceutical field for researchers to scout new drugs in drug discovery.
Knockdown of CNN3 Impairs Myoblast Proliferation, Differentiation, and Protein Synthesis via the mTOR Pathway
Authors: She Yanling , Li Cheng , Jiang Ting , Lei Si , Zhou Shanyao , Shi Huacai , Chen Rui 
Published: July 2021

BackgroundMyogenesis is a complex process that requires optimal outside–in substrate–cell signaling. Calponin 3 (CNN3) plays an important role in regulating myogenic differentiation and muscle regeneration; however, the precise function of CNN3 in myogenesis regulation remains poorly understood. Here, we investigated the role of CNN3 in a knockdown model in the mouse muscle cell line C2C12.MethodsMyoblast proliferation, migration, differentiation, fusion, and protein synthesis were examined in CNN3 knockdown C2C12 mouse muscle cells. Involvement of the mTOR pathway in CNN3 signaling was explored by treating cells with the mTOR activator MHY1485. The regulatory mechanisms of CNN3 in myogenesis were further examined by RNA sequencing and subsequent gene ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) and gene set enrichment analysis (GSEA).ResultsDuring proliferation, CNN3 knockdown caused a decrease in cell proliferation and migration. During differentiation, CNN3 knockdown inhibited myogenic differentiation, fusion, and protein synthesis in C2C12 cells via the AKT/mTOR and AMPK/mTOR pathways; this effect was reversed by MHY1485 treatment. Finally, KEGG and GSEA indicated that the NOD-like receptor signaling pathway is affected in CNN3 knockdown cell lines.ConclusionCNN3 may promote C2C12 cell growth by regulating AKT/mTOR and AMPK/mTOR signaling. The KEGG and GSEA indicated that inhibiting CNN3 may activate several pathways, including the NOD-like receptor pathway and pathways involved in necroptosis, apoptosis, and inflammation.
Racing Clean in a Tainted World: A Qualitative Exploration of the Experiences and Views of Clean British Elite Distance Runners on Doping and Anti-Doping
Authors: Shelley Jake , Thrower Sam N. , Petróczi Andrea 
Published: July 2021

Background: Doping has been a prominent issue for the sport of athletics in recent years. The endurance disciplines, which currently account for 56% of the global anti-doping rule violations in athletics, appear to be particularly high risk for doping.Objective: Using this high-risk, high-pressure context, the main purpose of this study was to investigate the human impact of doping and anti-doping on “clean” athletes. The secondary aim of the study was to better understand the reasons for, and barriers to, competing “clean” among this group of athletes.Method: Semi-structured interviews were conducted with 11 elite distance runners from the UK to explore: (1) the reasons and motivations for competing clean. (2) Perceptions of the anti-doping system, and experiences of being part of that system. (3) Views on the prevalence and causes of doping and the impact of doping on the lives of clean athletes. The interviews were audio-recorded, transcribed verbatim, and analysed using Reflexive Thematic Analysis.Results: Four major themes were identified: (1) The participants in this study have not been tempted to use prohibited substances or methods; they compete in their sport for the personal satisfaction of seeing how good they can be, rather than in pursuit of winning at all costs. (2) Anti-doping does not currently prevent doping effectively and is not implemented evenly across the globe. (3) Doping was perceived as a major issue and was felt to be borne out of certain sporting cultures in which doping is enabled. (4) Doping has impacted the careers of clean athletes in irreversible ways and presents a continuing challenge to the psychological preparation for competition.Conclusions: Clean athletes suffer negative consequences from both doping and anti-doping. ADOs must collaborate across borders to ensure a more even implementation of anti-doping activities, to facilitate a more level playing field on the global stage. ADOs must also acknowledge the existence of a large group of athletes who would never consider deliberately doping and make anti-doping work for these athletes too.
Factors Associated With Burnout, Post-traumatic Stress and Anxio-Depressive Symptoms in Healthcare Workers 3 Months Into the COVID-19 Pandemic: An Observational Study
Authors: Cyr Samuel , Marcil Marie-Joelle , Marin Marie-France , Tardif Jean-Claude , Guay Stéphane , Guertin Marie-Claude , Rosa Camille , Genest Christine , Forest Jacques , Lavoie Patrick , Labrosse Mélanie , Vadeboncoeur Alain , Selcer Shaun , Ducharme Simon , Brouillette Judith 
Published: July 2021

Objective: This study examined how best to identify modifiable protective and risk factors for burnout in healthcare workers in the face of the COVID-19 pandemic. Individual, occupational, organizational and social factors were investigated. The study also assessed the impact of these factors on post-traumatic stress disorder (PTSD), anxiety, and depression.Methods: Healthcare workers in the Quebec (Canada) healthcare system were recruited between May 21 to June 5, 2020. Participants answered an electronic survey 3 months after the COVID-19 epidemic outbreak began in Canada. Using the Maslach Burnout Inventory, PTSD Checklist for DSM-5, and Hospital Anxiety and Depression Scale, we studied the prevalence of burnout, PTSD, anxiety and depression in this cohort. Multivariable logistic or linear regression models including resilience, social and organizational support, workload and access to mental health help, simulation techniques and protective personal equipment (PPE) as well as perception of PPE security were conducted for each outcome.Results: In mid-June 2020, 467 participants completed the survey. We found that half (51.8%) of the respondents experienced burnout characterized by emotional exhaustion and/or depersonalization at least once a week. In total, 158 healthcare workers (35.6%) displayed severe symptoms of at least one of the mental health disorders (24.3% PTSD, 23.3% anxiety, 10.6% depression). Resilience (OR = 0.69, 95% CI: [0.55–0.87]; p = 0.002) and perceived organizational support (OR = 0.75, 95% CI: [0.61–0.93]; p = 0.009) were significantly associated with burnout and other outcomes. Social support satisfaction, perception of PPE security, work type and environment, mental health antecedents and reassignment were associated with PTSD and/or anxiety and/or depression, but not burnout.Conclusion: Future studies should address primarily resilience and perceived organizational support to promote mental health and prevent burnout, PTSD, anxiety and depression.
Mental and Social Health of Children and Adolescents With Pre-existing Mental or Somatic Problems During the COVID-19 Pandemic Lockdown
Authors: Zijlmans Josjan , Teela Lorynn , van Ewijk Hanneke , Klip Helen , van der Mheen Malindi , Ruisch Hyun , Luijten Michiel A. J. , van Muilekom Maud M. , Oostrom Kim J. , Buitelaar Jan , Hoekstra Pieter J. , Lindauer Ramón , Popma Arne , Staal Wouter , Vermeiren Robert , van Oers Hedy A. , Haverman Lotte , Polderman Tinca J. C. 
Published: July 2021

Background: The COVID-19 lockdown increases psychological problems in children and adolescents from the general population. Here we investigate the mental and social health during the COVID-19 lockdown in children and adolescents with pre-existing mental or somatic problems.Methods: We included participants (8–18 years) from a psychiatric (N = 249) and pediatric (N = 90) sample, and compared them to a general population sample (N = 844). Measures were assessed during the first lockdown (April-May 2020) in the Netherlands. Main outcome measures were Patient-Reported Outcomes Measurement Information System (PROMIS®) domains: Global Health, Peer Relationships, Anxiety, Depressive Symptoms, Anger, and Sleep-Related Impairment, as reported by children and youth. Additionally, socio-demographic variables, COVID-19-related questions, changes in atmosphere at home from a parent and child perspective, and children's experiences of lockdown regulations were reported by parents.Results: On all measures except Global Health, the pediatric sample reported least problems. The psychiatric sample reported significantly more problems than the general population sample on all measures except for Anxiety and Peer Relationships. Having a COVID-19 affected friend/relative and a COVID-19 related change in parental work situation negatively moderated outcome, but not in the samples with pre-existing problems. All parents reported significant decreases in atmosphere at home, as did children from the general population.Conclusion: We observed significant differences in mental and social health between three child and adolescent samples during the COVID-19 pandemic lockdown and identified COVID-19-related factors influencing mental and social health.
Globalization and Missionary Ambition in West African Islam. The Fayda after Sheikh Ibrahim Niasse
Authors: Niang Cheikh E. Abdoulaye 
Published: July 2021

For more than 10 years, we have been observing the Fayda Tijaniyya and its ramifications around the world. Starting in 2006 we have been conducting observations in Nigeria, Niger, Senegal and Mauritania. Between 2015 and 2017, we closely followed some developments of the Fayda in the French capital and in other European cities. In parallel to these field investigations, we have been interested in the new religious arrangements that are gradually emerging in the United Kingdom. More recently, in 2020, we benefited from a stay in the USA which allowed us to widen our observation framework. From there, we were able to collect empirical material consisting of several dozen interviews, direct observation notes, private and public documents (reports, administrative documents, correspondences, letters of recommendation, press articles, travel chronicles and private videos, among others). In the Fayda Ibrahimiyya, the global culture suggests an update of the mode of inheritance, but in addition it makes emerge a new framework of activity essentially composed of a device of formation with global character, of a hybrid diplomatic-religious space, finally of a humanitarian action which appears as a missionary presenza. We first show that the conjunction of these three registers of action has as a condition, a process of articulation and appropriation through which the actors of the Fayda manage to convince of the link of identity that prevails between their own history and that of the founder. The conclusion of the article is devoted to a critical discussion in which we postulate that the imagination of a homogeneous global community comes up against a form of local resistance that is a counterpoint and probably an anti-globalizing replica. The historical socio-anthropology of comprehensive inspiration that we favour allows us to finely approach the interdependencies between these three registers and the narrative elaborated locally by the supporters of this community. The paradigm of mobility in which we place the Fayda, allows us to grasp the scope of the globalization of practices and uses, as well as the tension that operates between a dynamic of continuity and a dynamic of rupture that crosses for some decades this religious community.
Maladie et pratiques thérapeutiques en contexte bouddhique arakanais (ouest de la Birmanie)
Authors: Coderey Céline 
Published: July 2021

En s’appuyant sur une étude de cas spécifique, cet article montre qu’en Arakan la maladie est un phénomène conçu comme résultant d’une imbrication de facteurs et géré par une variété de pratiques issues de différents domaines de savoirs et de techniques. Pour la soigner, malades et thérapeutes font appel aux ressources du bouddhisme theravāda, de l’astrologie, de l’alchimie, de la sorcellerie, des savoirs et pratiques ésotériques, de la médecine indigène et de la médecine scientifique d’origine occidentale — cela dans le cadre d’un parcours thérapeutique qui implique
une articulation entre ces différents domaines et dont la finalité est de garantir au
malade une protection-guérison aussi complète que possible en intervenant sur tous les facteurs à l’origine de la maladie.
Le site géologique de l’ancienne cité de Châtel-Aillon (Castrum Allionis) et l’évolution de la côte de l’Aunis
Authors: Mathieu G. 
Published: July 2021

Durant un séjour de six mois à Châtel-Aillon, tout en collectionnant les galets exotiques de la plage, l'auteur a pu observer les phénomènes littoraux et le régime de l'évolution du littoral d'Aunis.
Pour connaître les rivages successifs, il a dressé la carte du sous-sol à l'intérieur des terres depuis l'époque Gallo-Romaine, tandis que l'élude des falaises faite aux plus basses marées lui a permis de préciser certains points de tectonique sur les récifs du Cornard situés à 4 km. 5 en mer.
L'histoire locale, tracée par Amos Barbol, permet de dater exactement la suite des positions successives de la côte mieux que la faune des cordons littoraux, trop identique à la faune actuelle.
Celle élude montre donc l'utilité des faits historiques pour la géomorphologie d'une ligne de rivage en pleine évolution ; elle fait ressortir aussi l'influence de la tectonique sur la morphologie.