bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification the role of mono-ADP-ribosylation in colorectal cancer by integrated Transcriptome analysis
Authors: Zhang Shuxian , Duan Jiale , Yang Yanping , Gong Hanjuan , Tang Yi , Xiao Ming , Li Ming , Li Qingshu , Wang Ya-Lan 
Published: February 2021

Abstract
Purpose

Our previous study has clarified the carcinogenic properties of arginine-specific mono-ADP ribosyltransferase 1(ART1), which is considered to be a critical post-translational modification that changes the structure and function of proteins and is widely involved in important processes. This study provides, for the first time, a comprehensive insight of transcriptomic analysis for colorectal cancer cells interfered with ART1 silencing by Illumina RNA-Seq and related verification experiments.
Methods

Lentiviral infection was used to construct a CT-26 cell line that stably knocks down the ART1 gene, a whole transcriptome sequencing technique was performed to identify differentially expressed genes (DEGs). GO and KEGG classification/enrichment analysis and verification experiments were performed to determine the role of ART1 in the progression of colorectal cancer.
Results

a total of 5552 DEGs, GO function and KEGG pathway with highest enrichment, forms of SNP and diverse splicing patterns were able to be identified. Importantly, knockdown of ART1 affected the occurrence of the splicing of certain key genes related to tumor cell growth, also down-regulated expression of the key gene PTBP1 for alternative splicing. The overall attenuation of the endoplasmic reticulum unfolded protein response (UPR) signaling pathway caused by ART1 inhibition would unbalance UPR signaling, leading to the occurrence of apoptosis to impede tumorigenesis.
Conclusion

ART1, which clustered in organelles, may promote the development of colorectal cancer by participating in a variety of new mechanisms including endoplasmic reticulum stress regulation, metabolic process or alternative splicing, which may provide a good clinical drug candidate closer to targeted therapy of CRC.
Preoperative Prediction of The Operability of Pancreatic Carcinoma
Authors: Abdalla Wael , Abdellatif Tarek Ezzet , Safwat Khaled , Elmenshawy Hazem Hakim , Naguib Sameh 
Published: February 2021

Abstract
This work discussed the best diagnostic modility for early detection of pancreatic cancer compared to final diagnosis reached by histopathological examination. The present study was constructed by doing imaging modalities computed tomography (CT) and endoscopic ultrasonography (EUS) on 24 patients admitted to surgery department in the faculty of medicine with suspected pancreatic malignancy. As regard masses diameters detected by each modality, EUS was found to be superior to CT in detection of masses less than 20mm in diameter. According to the mass nature, those found in the head region tended to be malignant more frequently than the body and tail, Moreover, solid heterogeneous masses also were more likely to be malignant than the cystic ones as detected by the imaging modalities, with higher accuracy of EUS than with CT. EUS showed more accurate information as regards lymph node involvement and vascular invasion than CT. All of which affected the assessment of resectability of pancreatic masses. On head-to-head comparison, EUS appeared to perform better than CT scan in respect to nodal staging. Based on these results, we conclude that EUS staging should be the standard of care along with state of the art CT scan for the preoperative evaluation of patients with pancreatic cancer. However, large, prospective, multicenter studies directly comparing the accuracy of linear EUS vs. new generation CT scanners are still needed.
Experimental and Theoretical Study of Electrical Conductivity of Intermetallic and Nanocomposites: Subzero Temperature Superconductor
Authors: Kumar Harish , Bhateria Aarti , Singh Bhupender , Kaur Pawanvir 
Published: February 2021

Abstract
A comparative study of the electrical conductivity of intermetallic and corresponding nanocomposites at room and the subzero temperature was carried out by experimental and theoretical techniques. Four series of intermetallic compounds (IMCs) and nanocomposites (NCs) of Al2O3, ZnO, TiO2, and CuO were synthesized by high-temperature reactive synthesis and sol-gel technique, respectively. Heat treatment of NCs was carried out at 100, 200, 300, and 400°C. Characterization of NCs was carried out by UV-visible, FTIR, FE-SEM, and XRD techniques. Computational study (DMol3, CASTEP, Forcite, Reflex, and VAMP) was carried out to study the electronic and optical properties of metal nanoparticles. The influence of heat treatment, the addition of coinage metal and ferromagnetic material, effect of a decrease in temperature on the electrical conductivity of IMCs and NCs were thoroughly investigated. The electrical conductivity of NCs was found to increase with an increase in heat treatment temperature (up to 400°C) and with the decrease in temperature (-70°C). The addition of Hematite, α-Fe2O3, and coinage metal do not significantly increase the electrical conductivity of NCs at room temperature. The electrical conductivity of NCs was found to be higher than IMCs and coinage metals. Hence, synthesized NCs can have a large number of potential applications in nanowires, quantum dots, and nano cables with very low resistivity (10-6 ΩWm-1).
Flexible and anisotropically conductive sandwich film by assembly of liquid silicone rubber and cobalt coated glass fiber composites via magnetic field inducement
Authors: zhou ruihua 
Published: February 2021

Abstract
In this work, we have prepared electromagnetic cobalt coated glass fiber (Co@GF) composite via electroless plating method firstly. And then a conductive sandwich flexible film consisted of Co@GF composite and liquid silicone rubber (RTV-2) is successfully formed, through tape casting method at room temperature. Based on the perfect coating and excellent electrical conductivity of Co@GF composite, the resultant RTV-2/Co@GF/RTV-2 sandwich flexible film shows high electrical conductivity of 0.264 Ω·cm and can stretch to 100% ( of 4.40 Ω·cm) without obvious fracture. When the magnetic field was applied to the curing process, the electromagnetic Co@GF composite aligned automatically in the RTV-2 matrix on account of its ferromagnetic nature. The as-prepared film shows anisotropy in electrical performance. The volume resistivity parallel to the magnetic field direction is about two times lower than that of perpendicular direction. The maximum difference in volume resistivity (ρ∥ of 0.768 Ω·cm and ρ⊥ of 1.549 Ω·cm) is obtained at magnetic field intensity of 800 mT. In addition, a certain magnetic field intensity at 100mT is helpful to improve the electrical conductivity of the as-obtained sandwich film. The anisotropic RTV-2/Co@GF/RTV-2 sandwich flexible film would be considered as promising flexible electronic sensor where discrepant sensing sensitivity is required in orthogonal directions.
Genetic diversity analysis in indigenous rice (Oryza sativa L.) germplasm for Bacterial Leaf Blight (Xanthomonas oryzae pv. Oryzae) using resistance genes linked markers
Authors: . Khannetah K. R , S. Ramchander , T Andrew Peter Leon M. , D. Shobha , S. Saravanan , R. Kannan , Yasin Jeshima Khan , M. Arumugam Pillai 
Published: February 2021

Abstract
Among rice ( Oryza sativa L.) diseases, bacterial blight (BB), caused by Xanthomonas oryza pv. Oryzae , plays a pivotal role in decreasing rice yield. BB is reported to be the most serious constraint to improving rice yield. The present investigation assessed the potentiality of bacterial blight resistance and molecular characterization of 100 rice accessions for four major BB resistance genes, viz., Xa4, xa5, xa13 and Xa21 . Disease screening was carried out under glasshouse conditions using a BB culture isolated from BB-infected rice fields through the leaf clipping method. Analyses of 13 BB resistance genes linked with polymorphic microsatellites markers indicated the presence of single-, two-, three- and four-gene combinations of xa5, xa13, Xa4 and Xa21 . We found four accessions to be resistant; 34 accessions to be moderately resistant; 49 accessions to be moderately susceptible and 13 accessions to be susceptible. Among the resistant lines, IR12L110, Namcheonbyeo, Dhalaheera and SahbhagiDhan recorded a minimum lesion length of 3.7cm, 4.2cm, 4.67cm and 8.3 cm, respectively. Phylogenetic tree, constructed using molecular data, grouped the rice germplasm into four major clusters. R genes xa 5 and Xa4 contributed 14 positive compatible R genes, each belonging to 28 germplasm for BB resistance. The potential genetic resources identified as resistant to BB can be used as donors for the improvement of rice BB resistance in rice breeding programs.
Laser cladding treatment for refurbishing disc brake rotors – environmental and tribological analysis
Authors: Olofsson Ulf , Lyu Yezhe , Åström Anna Hedlund , Wahlström Jens , Senad Dizdar , Nogueira Ana Paula Gomes , Gialanella Stefano 
Published: February 2021

Abstract
In this study, grey cast iron disc brake rotors are refurbished by adding a surface layer through laser cladding. Current methods to deal with replaced rotors mainly include re-melting, with a minority fraction disposed in landfill. Both approaches result in a huge waste of resources and an increase in CO 2 footprint. From a sustainable point of view, this study aims to evaluate the feasibility of refurbishing brake rotors by a combined environmental and tribological performance approach. A streamlined life cycle assessment is conducted to compare the environmental impacts between producing virgin grey cast iron brake rotors and refurbishing replaced brake rotors by laser cladding. It turns out that the energy consumption and CO 2 footprint of the laser cladding refurbished brake rotors are 80% and 90% less than the virgin brake rotors. The results show that the refurbished brake rotor yields higher friction compared to the original cast iron utilizing the same pad material. The wear and particle emissions of the disc brake contact are in this study higher for the laser cladded one compared to the original cast iron one.
PO2RDF: Representation of Real-world Data for Precision Oncology Using Resource Description Framework
Authors: ZHAO Yiqing , Dimou Anastasios , Shen Feichen , Zong Nansu , Davila Jaime I. , Liu Hongfang , Wang Chen 
Published: February 2021

Abstract
Background: Next-generation sequencing provides comprehensive information about individuals’ genetic makeup and is commonplace in precision oncology practice. Due to the heterogeneity of individual patient’s disease conditions and treatment journeys, not all targeted therapies were initiated despite actionable mutations. To better understand and support the clinical decision-making process in precision oncology, there is a need to examine real-world associations of patients’ genetic information and treatment choice.Methods: To fill the gap of insufficient use of real-world data (RWD) in electronic health records (EHRs), we generated a single Resource Description Framework (RDF) resource, called PO2RDF (precision oncology to RDF) by integrating information regarding gene, variant, disease, and drug from genetic reports and EHRs. Results: There are total 2,309,014 triples contained in the PO2RDF. Among them 32,815 triples are related to Gene, 34,695 triples are related to Variant, 8,787 triples are related to Disease, 26,154 triples are related to Drug. We performed one use case analysis to demonstrate the usability of the PO2RDF: we examined real-world associations between EGFR mutations and targeted therapies to confirm existing knowledge and detect off-label use. Conclusions: In conclusion, our work proposed to use RDF to organize and distribute clinical RWD that is otherwise inaccessible externally. Our work serves as a pilot study that will lead to new clinical applications and could ultimately stimulate progress in the field of precision oncology.
Large Core Optical Elastomer Splitter Fabricated by Using 3D Printing Pattern
Authors: Prajzler Václav , Zavrel Jakub 
Published: February 2021

Abstract
The design, fabrication and properties of the large core 1x2Y optical planar splitter using optical elastomers for cladding and core is demonstrated. The splitters were designed by beam propagation method optimized for operation wavelength 650 nm. The splitters were fabricated using epoxy polymer pattern fabricated by Stereolithography 3D printing technology. The dimensions of the splitters were optimized for assembling optical fiber with core diameter 500 µm. The splitter shows optical losses around 1.48 dB at 650 nm, 1.18 dB at 850, 1.82 dB at 1300 nm and 2.12 dB at 1550 nm at room temperature.
Prostaglandin E2 Enhances Aged Hematopoietic Stem Cell Function
Authors: Patterson Andrea M. , Plett P. Artur , Sampson Carol H. , Simpson Edward , Liu Yunlong , Pelus Louis M. , Orschell Christie M. 
Published: February 2021

Abstract
Aging of hematopoiesis is associated with increased frequency and clonality of hematopoietic stem cells (HSCs), along with functional compromise and myeloid bias, with donor age being a significant variable in survival after HSC transplantation. No clinical methods currently exist to enhance aged HSC function, and little is known regarding how aging affects molecular responses of HSCs to biological stimuli. Exposure of HSCs from young fish, mice, nonhuman primates, and humans to 16,16-dimethyl prostaglandin E2 (dmPGE2) enhances transplantation, but the effect of dmPGE2 on aged HSCs is unknown. Here we show that ex vivo pulse of bone marrow cells from young adult (3 mo) and aged (25 mo) mice with dmPGE2 prior to serial competitive transplantation significantly enhanced long-term repopulation from aged grafts in primary and secondary transplantation (27% increase in chimerism) to a similar degree as young grafts (21% increase in chimerism; both p<0.05). RNA sequencing of phenotypically-isolated HSCs indicated that the molecular responses to dmPGE2 are similar in young and old, including CREB1 activation and increased cell survival and homeostasis. Common genes within these pathways identified likely key mediators of HSC enhancement by dmPGE2 and age-related signaling differences. HSC expression of the PGE2 receptor EP4, implicated in HSC function, increased with age in both mRNA and surface protein. This work suggests that aging does not alter the major dmPGE2 response pathways in HSCs which mediate enhancement of both young and old HSC function, with significant implications for expanding the therapeutic potential of elderly HSC transplantation.
Aquaporin 3 promotes human extravillous trophoblast migration and invasion
Authors: Nong Yingqi , Li Shifen , Liu Wenjuan , Zhang Xiqian , Fan Lin , Chen Ye , Huang Qianwen , Zhang Qianyu , Liu Fenghua 
Published: February 2021

Abstract
Problem: Does aquaporin 3 (AQP3) affect the migration and invasion of human extravillous trophoblast (HTR8/Svneo) cells?Method of Study: A lentivirus infection system was used to construct stable cell lines with either AQP3 knockdown or overexpression. RT-PCR and western blotting were used to verify the efficiencies of AQP3 knockdown or overexpression in HTR8/Svneo cells at mRNA and protein levels, respectively. Cell Counting Kit-8 and flow cytometry assays were used to detect the influence of AQP3 knockdown or overexpression on proliferation and apoptosis of HTR8/Svneo cells. In addition, wound healing and Transwell invasion assays were used to detect the effects of AQP3 knockdown or overexpression on migration and invasion capabilities of HTR8/Svneo cells. An Agilent gene chip was used to screen for significant differentially expressed genes after AQP3 knockdown. Finally, mechanisms by which AQP3 influences the migration and invasion of HTR8/Svneo cells were explored using bioinformatic analysis.Results: Compared with controls, migration and invasion capabilities of HTR8/Svneo cells were significantly reduced after AQP3 knockdown, and significantly increased after AQP3 overexpression. Subsequent bioinformatic analysis of gene chip expression profiles indicated downregulation of genes related to adhesion such as PDGF-B, as well as signaling pathways (such as PIK3/AKT, NF-κB, and TNF) after AQP3 knockdown. Conclusions: AQP3 could significantly promote migration and invasion capabilities of human extravillous trophoblasts, it may mediate embryo invasion and adhesion to endometrium by regulating PDGF-B, PIK3/AKT signaling pathways, although this requires further verification.
Episode Five: Omikemi
Authors: Shah Rajni , Omikemi  , 周 Gibbons Fili 
Published: February 2021

In this final episode of the podcast, Rajni Shah and Omikemi engage in a long, slow, wide conversation, in which they reflect on the entanglements between the felt world of lived experiences and the systems within which we live our lives. Infused by the sounds and shapes of the ocean, this episode feels intimate and visceral – and perhaps more than any of the others, like an invitation to eavesdrop on a conversation between friends.
In the accompanying offering, Omikemi invites listeners to spend time with a question that was present for them at the time of recording.
Full credits and transcript for this episode will be published when the episode is released on 27 April 2021.
The Putative Adverse Effects of Bisphenol A on Autoimmune Diseases
Authors: Sharif Kassem , Kurnick Adam , Coplan Louis , Alexander Matthew , Watad Abdulla , Amital Howard , Shoenfeld Yehuda 
Published: February 2021

:
Bisphenol A (BPA) is a monomer that is widely used in the manufacturing of polycarbonate
plastics (including storage plastics and baby bottles) and is considered to be one of the most
widely used synthetic compounds in the manufacturing industry. Exposure to BPA mainly occurs
after oral ingestion and results from leaks into food and water from plastic containers. According to
epidemiological data, exposure is widespread and estimated to occur in 90% of individuals. BPA
exhibits pleiotropic and estrogen-like effects; thus, it is considered an endocrine-disrupting chemical.
A growing body of evidence highlights the role of BPA in modulating immune responses and
signaling pathways, which results in a proinflammatory response by enhancing the differential polarization
of immune cells and cytokine production profile to one that is consistent with proinflammation.
Indeed, epidemiological studies have uncovered associations between several autoimmune
diseases and BPA exposure. Data from animal models provided consistent evidence, which highlighted
the role of BPA in the pathogenesis, exacerbation, and perpetuation of various autoimmune
phenomena including neuroinflammation in the context of multiple sclerosis, colitis in inflammatory
bowel disease, nephritis in systemic lupus erythematosus, and insulitis in type 1 diabetes mellitus.
Owing to the widespread use of BPA and its effects on immune system dysregulation, a call
for careful assessment of patients’ risks and public health measures are needed to limit exposure
and subsequent deleterious effects. The purpose of this study is to explore the autoimmune triggering
mechanisms and present the current literature supporting the role of BPA in the pathogenesis of
autoimmune diseases.
Investigating Fill Factor Effect on Brillouin Zone of Metamaterial-Based 2D Photonic Crystal
Authors: Deyasi Arpan , Ghosh Ratul , Chakraborty Papri , Adhikary Anwesha , Sarkar Angsuman 
Published: February 2021

:
Fill factor in the negative permittivity materials are tailored within the physically permissible
limits to characterize the Brillouin zone for two-dimensional crystal under the propagation of both s and
p-polarized waves. Two lowermost band-gaps are computed along with corresponding mid-band frequencies,
where the plane wave expansion method is invoked for computational purposes. The rectangular
geometrical shape is considered for the simulation, and all the results are calculated inside the ‘Γ’
point and ‘X’ point of the first Brillouin zone. Simulated findings depict monotonous variations of both
band-gap width as well as mid-band frequency for higher negative permittivity materials, when the
magnitude of fill factor is comparatively low, for both TE (Transverse Electric) and TM (Transverse
Magnetic) mode of propagations. Lower negative permittivity leads to random fluctuations, which
makes it unsuitable for photonic component design. Multiple forbidden regions may be observed for
some specific artificial materials which can be utilized in the antenna or multi-channel filter design in
higher THz region.

Aims:
The present paper aims to compute the shape of the first Brillouin zone from the fill factor for a
two-dimensional photonic crystal structure.

Background:
EBG (Electromagnetic Bandgap) of a photonic crystal plays a major role in determining
its candidature for optical applications, which is critically controlled by the fill factor. Therefore, it is
significant to investigate the effect of F.F on the wave propagation characteristics of 2D PhC(Twodimensional
photonic crystal).

Objective:
Investigation of metamaterial-based photonic crystal structure for electromagnetic band-gap
analysis in the desired spectrum of interest as a function of fill factor inside the first Brillouin zone.

Methods:
Maxwell’s equations are solved using the plane wave propagation method to solve the problem,
and simulation is carried out in MATLAB® software.

Result:
Both the first and second photonic band-gaps are simultaneously computed with a variation of
refractive index differences of the constituent materials as well as with the fill factors. Results are extremely
significant about the formation of narrowband and wideband filters on certain material combinations
and structural designs.

Conclusion:
Better tenability is observed for metamaterial structure compared to conventional positive
index materials, and fill factor has a great role in shaping the Brillouin zone and corresponding bandgap
width.
Chemical Constituents and Biological Activities of Cordia myxa L.: A Review
Authors: Singh Varinder , Sood Ankita , Pruthi Simran , Singh Manjinder , Saini Balraj , Singh Thakur Gurjeet , Kumar Amit 
Published: February 2021

Background:
Cordia myxa L. (CM) is a valuable ethnomedicinal plant from Boraginaceae family. Traditionally, CM parts especially fruits and leaves are used in chest and urinary tract infections, diarrhoea, dysentery, tuberculosis, liver and spleen disorders, chronic fever, malaria etc.

Objective:
Despite of known importance and uses, CM has gained relatively less attention of researchers and concise reviews revealing the medicinal potential of CM are scanty. The present review summarizes the chemical constituents and biological activities of CM and aims to stimulate future research to develop it as a functional health food.

Results:
Analysis of literature on CM showed that its fruits are a rich source of nutrients and are frequently employed in wide ailments such as urinary and respiratory tract infections, chronic fever, liver disorders, asthma, used as anthelmintic, diuretic, expectorant and purgative. Scientific studies have shown the antidiabetic, analgesic, anti-inflammatory, anti-cancer, antioxidant, antiplasmodial, hepatoprotective, hypotensive, antiulcer and antimicrobial activities of CM. More than 45 compounds belonging to carbohydrates, steroids, carotenoids, phenols, flavonoids and alkaloids have been reported from various parts of CM.

Conclusion:
Systematic preclinical studies support the traditional claims of CM. The analysis of available literature showed that CM could be developed as a drug. Further, studies such as detailed pharmacological and toxicological evaluation, isolation of bioactive compounds, quantitative phytochemistry and structure activity relationship are scanty and thus, crucial to be addressed for uplifting the scientific value of this revered medicinal plant.