bluuebunny/crossref_metadata_2025_split huggingface dataset:
Genetic Chinese Medicine Network Pharmacology Research, Quercetin Improves Drug Resistance in Acute Myeloid Leukemia by Targeting CXCL10
Authors: Linjun Hu , LU QILIANG , LIU YANG , ZHAO JUNJUN , ZENG ZHI , SHI ZHAN , TU YIFENG , XIAO ZUNQIANG , XU QIURAN , TONG XIANGMIN 
Published: March 2021

Abstract
Background: Acute myeloid leukemia (AML) is the most common acute leukemia in adults and is a highly heterogeneous and fatal disease. At present, the main method of treatment of AML is chemotherapy, but patients who relapse often develop resistance and are not sensitive to chemotherapy. Chinese medicine network pharmacology can provide new ideas about improving AML resistance.Methods: The gene expression data of relapsed drug-resistant AML and primary AML are from Gene Expression Omnibus (GEO) database. Based on the network pharmacology of traditional Chinese medicine, the effective components and target genes of Jiedu Huayu Decoction were analyzed. we performed Kyoto Encyclopedia of Genes and Genomes (KEGG), Gene Ontology (GO) analyses, Protein-protein interaction (PPI) network and construction on overlapping genes. We will perform prognostic analysis and gene correlation analysis of overlapping genes in GEPIA. The binding energy between the differential gene and the active ingredient of the drug was studied by molecular docking.Results: We found that quercetin, the active ingredient in Jiedu Huayu Decoction, can target CXCL10, thereby improving AML resistance.Conclusions: In this study, we found that quercetin improves drug resistance in acute myeloid leukemia by targeting CXCL10 based on the GEO database and the network pharmacology study of Chinese medicine.
Clinical Characteristics and Outcomes of Rare Fungal Keratitis Caused by Verticillium dahliae
Authors: Huang Qing , Zhang Wenlu , Sun Yu , Li Xiaofeng , Zhang Xiaoyu , Lu Xiuhai , Qi Xiaolin 
Published: March 2021

Abstract
PurposeTo observe clinical characteristics and treatment outcomes of fungal keratitis cause by Verticillium dahliae. MethodsClinical data of 7 patients diagnosed as fungal keratitis cause by V. dahliae were retrospectively analyzed. The clinical manifestations, mycology, in vitro antifungal susceptibility, treatment regimens and prognoses of the patients were evaluated. ResultsAll 7 patients were farm worker, of which 5 cases were caused by plant trauma. The corneal ulcer had a round shape and a relatively limited range with the diameters mainly in the range of 2-7 mm. The stromal infiltration was mild, and had no pseudopodia, mossiness or endothelial plaques. Intact hyphaes were detected in corneal scrapings and confocal microscopy, isolates were identified by morphology and by sequencing the internal transcribed spacer region of ribosomal DNA. In vitro antifungal susceptibility testing showed that the most sensitive antifungal drug was Amphotericin B. In the 6 patients with an ulcer less than 2/3 of the corneal thickness, the ulcer healed after 18 days of antifungal treatment only in one eye. The other five patients underwent corneal ulcer debridement or conjunctival flap covering surgery. The remaining one patient with ulcer depth more than 2/3 of the corneal thickness underwent lamellar keratoplasty. ConclusionFungal keratitis caused by V. dahliae has typical signs of a mild inflammatory response, and is not sensitive to antifungal drugs. It is recommended that patients undergo corneal ulcer debridement as soon as possible to promote rapid healing of the ulcers.
RNA methylation-mediated LINC01559 suppresses colorectal cancer progression by regulating the miR-106b-5p/PTEN axis
Authors: Shi Ke , Yang Shuaixi , Zhou Quanbo , Chen Chen , Shao Bo , Guo Yaxin , Wu Xiaoke , Zhao Luyang , Yang Xiuxiu , Zhang Qiuge , Wang Guixian , Yuan Weitang , Liu Jinbo , Sun Zhenqiang 
Published: March 2021

Abstract
BackgroundLong noncoding RNAs (lncRNAs) regulate multiple biological effects in cancers. Recently, RNA methylation has been found to modify not only coding RNAs but also some noncoding RNAs. How RNA methylation affects lncRNAs to affect colorectal cancer (CRC) progression remains elusive.MethodsRNA-sequencing of cancer and normal tissues was analysed. LINC01559 in CRC was selected and verified by western blot (WB), quantitative real-time PCR (qRT-PCR) and in situ hybridization (ISH). Functional experiments in vitro and in vivo were used to explore the biological functions of LINC01559 in CRC. The LINC01559/miR-106-5p/PTEN axis was explored at different levels of analysis, including fluorescence in situ hybridization (FISH), luciferase assays, and rescue experiments. RIP-sequencing, m6A RNA immunoprecipitation (MeRIP) assays and bioinformatic analysis were conducted to determine the upstream mechanism of LINC01559.ResultsLINC01559, identified by RNA-sequencing analysis, was downregulated in CRC tissues and cell lines compared with normal controls. Lower expression of LINC01559 in CRC patients predicted a poor prognosis. In addition, PTEN was found to be positively correlated with LINC01559, and bioinformatic analysis and in vitro experiments indicated that miR-106b-5p could be the link between LINC01559 and PTEN. Then, downregulated LINC01559 restored the malignant phenotype of CRC cells in vivo and in vitro, while cotransfection of si-LINC01559 and miR-106b-5p inhibitor neutralized this effect. Notably, METTL3 was found to be highly expressed in CRC, and the downregulation of METTL3 could inhibit the migration and invasion of CRC cells. Mechanistically, we found abundant m6A modification sites on LINC01559. Then, we uncovered these sites as potential targets of METTL3 through experiments in vivo.ConclusionThe results revealed a negative functional regulation of LINC01559/miR-106b-5p/PTEN axis in CRC progression, and explored a new mechanism of METTL3-mediated m6A modification on LINC01559 formation. These results elucidate a novel potential therapeutic target for CRC treatment.Trial registrationThe human cancer tissues used in this study were approved by Ethnics Committee of The First Affiliated Hospital of Zhengzhou University in December 19, 2019, and the TRN is 2019-KW-423.
Yaw stability control of automated guided vehicle under the condition of centroid variation
Authors: Liu Wei , Zhang Qingjie , Wan Yidong , Liu Ping , Yu Yue , Guo Jun 
Published: March 2021

Abstract
The centroid of an automated guided vehicle changes due to the irregular position and unbalanced weight of the merchandises on the load platform, which affects the completion of the handling task between stations in intelligent factories. This paper presents a hierarchical control strategy to improve yaw stability considering centroid variation. Firstly, the vehicle body and hub motor models are established based on dynamics. Secondly a hierarchical controller is designed by using the method of extension theory, model predictive control (MPC) and sliding mode control. Then based on CarSim and Simulink, the step co-simulation of the low-speed condition of the automated guided vehicle is carried out. Compared with the uncontrolled condition, the maximum deviation of the yaw rate is reduced from 0.58 rad/s to 0.52 rad/s, and the error with the theoretical value is reduced from 16% to 4%; the maximum deviation of the centroid sideslip angle is reduced from -0.84 rad to -0.77 rad, and the error with the theoretical value is reduced from 12% to 3%. Finally, a four-wheel drive and four-wheel steering automated guided vehicle is manufactured to carry out inter station steering experiments in simulated factory environment. The error between simulation and experiment is less than 5%. The results show that the designed controller is effective, and the research can provide theoretical and experimental basis for the low-speed steering control stability of automated guided vehicle.
Patients Acceptance and Comprehension to Written and Verbal Consent (PAC-VC)
Authors: Kashur Rabia , Ezekowitz Justin , Kimber Shane , Welsh Robert 
Published: March 2021

Abstract
Background:Acute myocardial infarction (AMI) research is challenging as it requires enrollment of acutely ill patients. Patients are generally in a suboptimal state for providing informed consent. Patients’ understanding to verbal assents have not been previously examined in AMI research. Patients Acceptance and Comprehension to Written and Verbal Consent (PAC-VC) compared patients’ understanding and attitudes to verbal and written consents in AMI RCTs.Methods: PAC-VC recruited patients from 3 AMI trials using both verbal N=12 and written N=6 consents. We compared patients’ understanding using two survey questionnaires. The first questionnaire used open-ended questions with multiple choice answers. The second questionnaire used a 5-point Likert scale to measure patients understanding and attitudes to the consent process. Overall answers average scores were categorized into three groups: Adequate understanding (71-100) %, Partial understanding (41-70)% and Inadequate understanding (0-40)%. Results:Responses showed patients with verbal assent had adequate understanding to most components of informed consent, close to those of written consent. Most patients did not read written information entirely and believed that it is not important to make a final decision. Patients favoured to have written information be part of the consent but not necessarily presented during the initial consent process. Patients felt less pressured in the verbal assent arm than those of written consent.Conclusion: Patients had adequate understanding to most components of verbal assent and comparable to those of written consent. Utilizing verbal assents in the acute care setting should be further assessed in larger trials.
Polymicrobial and Monomicrobial Necrotizing Fasciitis: Clinical, Laboratory, Radiology, Pathological Hallmark and Differences, a Retrospective Analysis
Authors: Naamany Eviatar , Shiber Shachaf , Daskin-Bitan Hadar , Yahav Dafna , Bishara Jihad , Sagy Iftach , Granat Nadav , Drescher Michael 
Published: March 2021

Abstract
Background: Necrotizing fasciitis(NF) is a life-threatening infection with high morbidity and mortality rates which should be diagnosed and treated with surgical and antibiotic therapy. Many studies have addressed NF and its subtypes, but few have reviewed the clinical, radiological and pathological differences between the poly-microbial and the mono-microbial groups. Objective: The objective of our study is to describe a relatively large cohort of patients with NF and study and compare the clinical, radiological and pathological differences between the poly-microbial(Pm) and the mono-microbial(Mm) groups.Methods: The charts of hospitalized patients with NF diagnosis from 2002-2019 at the Rabin Medical Center were reviewed. The primary outcome was all-cause mortality at 90 days, secondary outcomes included duration of hospitalization, intensive care unit(ICU) admission, LRINEC score and the need for vasopressor use. Results: 81 patients with NF were included in the study, 54(66.6%) had Mm growth and 27(33.3%) had Pm growth. There were no significant differences between the two groups in the 90 days mortality, and moreover in hospital mortality was also insignificantly different. In a multivariate analysis, we found that 90 days mortality was more prevalent in the Mm group compared to Pm group. In addition, we found that in hospital mortality, ICU admission and vasopressors use were more frequent among the Mm-group compared to the Pm-group. Conclusions: our study is the first to compare the differences between the two most prevalent entities of NF. The results demonstrate better prognosis for Pm-NF, with minimal ICU stay, lower mortality, and lower use of vasopressors.
Plants used for grilling fish in two ethnic groups in Guizhou, Southwest China
Authors: He Jianwu , Lan Daoying , Zhang Ruifei , Bai Yu , Long Chunlin 
Published: March 2021

Abstract
Background

Dong and Miao, two major ethnic groups in Qiandongnan Prefecture of Guizhou Province, China, have practiced an ancient cooking technique, grilling fish or fish barbecue, to boost their spirits and enrich their spiritual life as well. Using a variety of edible plants and fish from the famous rice-fish agricultural ecosystem, they made the grilled fish as sacrificial objects and food for the festivities. The knowledge of plants used for grilling fish has not been studied.
Methods

Data were collected using direct observation, semi-structured interviews, individual discussions, key informant interviews and focus group discussions administered to 279 informants living in ethnic villages of Congjiang County between September 2017 and March 2019. The fish-grilling plant voucher specimens were collected in the field excursions. The data were analyzed using ethnobotanical descriptive statistics, fidelity level (FL), and the cultural important plants index (CII). In addition, a cultural important plants matrix design was used for comparative analysis of the plant cultural importance among two major ethnic groups.
Results

A total of 52 plant species in 21 families and 43 genera were recorded as edible plants for fish-grilling. The families Lamiaceae, Apiaceae and Lauraceae were more important sources among all plant groups. Herbaceous plants were the most species for fish-grilling, and leaves were the main part used. Wild edible plants were mainly collected for fish-grilling and a small number of species were from kitchen gardens, such as Coriandrum sativum, Luffa aegyptiaca and Lagenaria siceraria. Both Dong and Miao people had a high-fidelity level towards fish-grilling plants, which some given species received higher cultural important plants index in two groups. The integration of formal education and traditional culture in the local communities is conducive to the transmission of local knowledge. The traditional culture of the Dong and Miao’s fish-grilling was very commonly related. The cultural preference is not particularly strong. Only a few species represent the characteristics of each group. In addition, this traditional barbecue ceremony was related to the ancestor worship of local people.
Conclusion

The present study is the first systematic ethnobotanical survey of traditional fish grilling knowledge in Congjiang County, Guizhou Province, China, which shows close relationship between local residents and local plant resources. Results from this study will be helpful to protect agricultural cultural heritage of fish-grilling plants in southeast Guizhou, and provide a basis for the development of new food types.
Genome-resolved metagenomics suggests a mutualistic relationship between Mycoplasma and salmonid hosts
Authors: Rasmussen Jacob Agerbo , Villumsen Kasper Rømer , Duchêne David A. , Puetz Lara Christine , Delmont Tom O. , Sveier Harald , Præbel Kim , Jørgensen Louise von Gersdorff , Martin Michael D. , Gilbert M. Thomas P. , Bojesen Anders Miki , Kristiansen Karsten , Limborg Morten Tønsberg 
Published: March 2021

Abstract
Salmonids are important sources of protein for a large proportion of the human population. Interaction between the gut microbiota and host has been shown to affect the host phenotype in mammals, but relatively little is known about microbiota-host interaction in fish. Mycoplasma species are a major constituent of the gut microbiota of salmonids, often representing the majority of microbial cells. Despite the frequent reported dominance of intestinal Mycoplasma species, very little is known about their phylogenetic placement, functions and potential evolutionary relationships with their salmonid hosts.In this study, we utilise 2.9 billion metagenomic reads generated from 12 samples from three different salmonid host species to I) characterise and curate the first metagenome-assembled genomes (MAGs) of Mycoplasma dominating the intestines of three different salmonid species, II) establish the phylogeny of these salmonid candidate Mycoplasma species using known Mycoplasma genomes, III) perform a comprehensive pangenomic analysis of Mycoplasma, IV) decipher the putative functionalities of the salmonid MAGs and reveal specific functions expected to benefit the host.Our data provide a basis for future studies examining the composition and function of the salmonid microbiota, with a potential for being further exploited in order to increase animal health and growth in aquaculture.
Effect of domestication on phenotypic plasticity in chaya (Cnidoscolus aconitifolius (Mill) I.M. Johnstone)
Authors: Munguía-Rosas Miguel A. 
Published: March 2021

Abstract
It is widely recognized that phenotypic plasticity may increase the survival of plants in environmentally variable habitats. However, the manner in which artificial selection affects the phenotypic plasticity of crops is poorly understood and there is no previous study in clonally propagated crops. The goal of this study was to assess the effect of domestication on phenotypic plasticity in leaf traits, growth rates and allocation patterns, under contrasting light environments, in the clonally propagated crop chaya (Cnidoscolus aconitifolious (Mill) I.M. Johnstone). Leaf area, leaf perimeter, leaf specific area, leaf production, trichome density, growth in height, growth in stem diameter and slenderness were measured in 97 clones belonging to 20 genotypes (10 wild and 10 domesticated). The clones were allocated to two contrasting light environments: fully exposed to sun vs. placement beneath a shade cloth. Reaction norms for each trait were compared between wild and domesticated clones. Results suggest that domestication and light environment had a non-additive effect on phenotypic plasticity in leaf production and slenderness. Reduced plasticity in these traits was observed in domesticated plants compared to that of their wild relatives. Leaf production and slenderness are associated with the shade avoidance syndrome, which is evident in wild plants but not manifested in domesticated plants. Reduced plasticity in leaf production also suggests yield stability, since the leaves form the edible part of chaya. I conclude that artificial selection reduces phenotypic plasticity in the yield of chaya and in its response to variation in the light environment.
Efficacy and Safety of Neoadjuvant Immunotherapy in Triple Negative Breast Cancer: Meta-analysis of randomized controlled trials
Authors: Ergun Yakup , Karacin Cengiz , Akagunduz Baran , Turker Sema , Acikgoz Yusuf , Dirikoc Merve , Esen Selin Akturk , Ucar Gokhan 
Published: March 2021

Abstract
Aim: In this meta-analysis; The efficacy and safety of randomized controlled trials (RCTs) using immunotherapy in the neoadjuvant treatment of triple negative breast cancer were investigated.Material and Method: To determine the studies to be evaluated in this meta-analysis, PubMed, Cochrane Library databases, and the studies published/presented in ASCO, ESMO, and SABCS congresses were thoroughly searched. Studies published/presented until January 01, 2021, were included. PCR, by definition, was defined as the absence of invasive cancer cells in the breast and axillary lymph nodes (ypT0 / Tis and N0). If there were at least two studies that shared the same subgroup results, subgroup analyzes were also performed. This meta-analysis was performed using Review Manager, version 5.4 (RevMan), a proprietary software provided by Cochrane Collaboration.Results: A total of 5 RCTs were included in the study, and 1498 patients were analyzed. In a pooled analysis of these studies, pCR was 58.8% in the immunotherapy arm and 42.6% in the placebo arm. According to the random-effects model, OR was found to be 1.77 (95% CI 1.23-2.56). In the pooled analysis of 3 studies reporting results according to PD-L1 level, immunotherapy significantly increased pCR in the PD-L1 positive group (67% vs. 49%, OR: 1.99, 95% CI 1.47-269). In the PD-L1 negative group, although immunotherapy increased pCR numerically, it was not statistically significant (42% vs. 33%, OR: 1.44, 95% CI 0.97-2.14). According to the lymph node status, the results were shared in 2 studies. In the joint analysis of these two studies, pCR was significantly increased with immunotherapy in those with positive lymph nodes (63% vs. 39%, OR: 2.52 95% CI 1.69-3.77). In those with negative lymph nodes, although immunotherapy increased pCR numerically, the difference was not significant (62% vs. 54%, OR: 1.36 95% 0.94-1.97).Conclusion: The addition of immunotherapy to neoadjuvant chemotherapy increases pCR. This increase was especially pronounced in high-risk patients with lymph node-positive and in the group with positive for PD-L1.
Managing everyday life with chronic pain and interaction with healthcare: a qualitative study
Authors: Ranås Ebba , Dahlrot Amanda , Grimby-Ekman Anna , Ahlstrom Linda 
Published: March 2021

Abstract
Background: Chronic pain is a costly, widespread condition that often reduces the quality of life of individuals who suffer from it, for whom healthcare interaction is seldom efficient or free of problems. Outcomes of coping with chronic pain can depend upon patients’ approaches towards their pain, their defensive or offensive behaviours and their choices based upon their self-efficacy. Thus, even when their symptoms and diseases seem homogeneous, patients should be regarded as multidimensional individuals and their care plans should be individually tailored. The aim of our study was to examine how individuals with chronic pain manage their everyday lives and, from their perspectives, how primary care organisations should support them. Method: We conducted qualitative content analysis on the written narratives of 21 patients with chronic pain and held a focus group discussion with 15 of them. Results: The overarching theme in the patients’ narratives was that individuals with chronic pain develop adaptability in managing their everyday lives. Therein, the first sub-theme captures how such individuals use their bodies and souls to condition themselves and adapt to the people around them, take control of their everyday lives and find empowerment, whereas the second sub-theme captures how they interact with primary care organisations, their experiences with such interaction and the need to be treated with respect. Conclusions: For patients living with chronic pain, managing everyday life, the accompanying challenges and ongoing interactions with healthcare organisations requires developing adaptability. Although the individuals indeed bear great responsibility for their own health and wellbeing, healthcare personnel can ease that burden by adopting person-centred approaches in their care. However, to that end, healthcare personnel need more knowledge about supporting individuals living with chronic pain in order to ease their suffering.