bluuebunny/crossref_metadata_2025_split huggingface dataset:
Molecular study of Nucleophosmin 1(NPM1) gene in acute myeloid leukemia in Kurdish population
Authors: Obaid Othman Galawezh , Sadiq Mohammad Nawsherwan , Hameed Saeed Chiman 
Published: August 2021

Background: In patients with Acute Myeloid Leukemia (AML) the most frequent acquired molecular abnormalities and important prognostic indicators is nucleophosmin-1 (NPM1) mutations. Our study aims was molecular study of Nucleop- hosmin -1 gene in Acute Myeloid Leukemia in Kurdish population.
Patients &Methods: A total of 50 patients with AML, (36) of them attended Nanakaly Hospital and (14) attended Hiwa Hospital and 30 healthy subjects as control were selected randomly, all were matched of age and gender. Polymerase chain reaction (PCR) was used for detection of NPM1 gene mutation. Three samples of PCR product for NPM1 gene mutations were sequenced, and mutations were determined by comparison with the normal NPM1 sequence NCBI (GenBank acces- sion number NM_002520).
Results: Out of 50 patients with AML, 5 (10%) of them were NPM1 gene mutation positive, and 45 (90%) were negative. The mutation were a base substitution (C to A), (G to C), (G to T), transversion mutation in addition of frame shift mutation and all mutated cases were heterozygous and retained a wild type allele.
Conclusion: Identification of NPM1 mutations in AML are important for prognostication, treatment decision and optimi- zation of patient care.
Keywords: Acute myeloid leukemia; Nucleophosmin-1 (NPM-1) gene mutation; PCR.
Circulatory microRNA expression profile for coronary artery calcification in chronic kidney disease patients
Authors: Vijayaraghavan Bhooma , Jeyamohan Sridharan , Padmanabhan Giri , Joseph Velangann Antony , Ramanathan Kumaresan 
Published: August 2021

Background & Aim: Coronary artery disease (CAD) is the primary cause of mortality in patients with end stage renal disease (ESRD). MicroRNA profiling is proven as a powerful tool in the diagnosis of any disease at the molecular level. Hence, the present study aimed to profile the microRNA expression for CAD especially coronary artery calcification in CKD patients.
Materials and Methods: Two hundread patients with CKD stages 3 to 5 without dialysis and healthy controls were includ- ed in this study. All two hundred patients underwent 1024 multi sliceardiac computed tomography (CT) scan for calcium scoring. The calcium scoring more than 100 have been included in the study. We performed miRNA microarray analysis from serum samples of seven high calcium scored with CKD patients and one control patients.
Results: Seven patients have observed circulating miRNAs has significantly upregulated and downregulated when compared with control patients. mir21, mir 67, mir 390, mir 56, mir 250, mir 65 and mir 13 were up regulated and mir235, mir256, mir226, mir207, mir255, mir193 were downregulated. There was no significant difference in left ventricle function.
Conclusion: 13 microRNAs play a potential role in coronary artery calcification in CKD patients.
Keywords: CKD; CAD; microRNA; coronary artery calcification.
Nutrition education improves knowledge and BMI-for-age in Ghanaian school-aged children
Authors: A Annan Reginald , Apprey Charles , O Agyemang Godwin , M Tuekpe Diane , Asamoah-Boakye Odeafo , Okonogi Satoru , Yamauchi Taro , Sakurai Takeshi 
Published: August 2021

Background: Adequate nutrition is required for growth and development in children. This study tested the effectiveness of nutrition education on knowledge and BMI-for-age (BFA) of school-aged children in the Kumasi Metropolis.
Methods: Children, aged 9-13 years old were recruited from ten randomly selected primary schools in the Metropolis. The schools were randomly allocated into 3 groups: nutrition education (3 schools), physical activity (PA) education (3 schools), both interventions (2 schools), or control (2 schools). Following a baseline nutrition and PA knowledge and status assess- ment in 433 children, twice-monthly nutrition and PA education and demonstrations were carried out for 6 months, followed by a post-intervention assessment.
Results: PA and nutrition knowledge improved in all groups (P<0.001); the highest improvement was among those who received both interventions (31.0%), followed by the nutrition education group (29.8%), and the least, the control group (19.1%). Overall, BFA improved by +0.36, from baseline (-0.26) to end of the intervention (+0.10, P<0.001). Within the groups, the nutrition group (+0.65, P<.001) had the highest improvement, then, both the intervention group (+0.27, P<0.001), the PA group (+0.23, P<0.001) and lastly, the control group (+0.18, P=0.001).
Conclusion: Nutrition education could improve knowledge and BMI-for-age in school-aged children in Ghana.
Keywords: School-aged children; nutrition education; BMI-for-age; nutrition knowledge; basic school.
Clinical description and mutational profile of a Moroccan series of patients with Rubinstein Taybi syndrome
Authors: Chafai Elalaoui Siham , Smaili Wiam , Van-Gils Julien , Fergelot Patricia , Ratbi Ilham , Tajir Mariam , Arveiler Benoit , Lacombe Didier , Sefiani Abdelaziz 
Published: August 2021

Background: Rubinstein-Taybi syndrome (RSTS; OMIM 180849) is a rare autosomal dominant developmental disorder with an estimated prevalence of one case per 125,000 live births. RSTS is characterized by typical face, broad thumbs and halluces, short stature, and intellectual disability. Facial dysmorphy is characteristic with microcephaly, low frontal hairline, arched eyebrows, long eyelashes, convex profile of nose, narrow palate, and micrognathia. RSTS is mainly due to mutations or microdeletions of the CREBBP gene (about 60%) and more rarely of the EP300 gene (8%).
Objective: Clinical description and identification of mutations of patients with Rubinstein Taybi syndrome.
Methods: PCR and direct sequencing of CREBBP gene.
Results: We report here, the clinical and molecular data of a series of six Moroccan patients with a phenotype of RSTS. The molecular study of the major gene CREBBP (by Sanger Sequencing followed by CGH array, if sequence normal) revealed point mutations in five patients. For the sixth patient, CGH array revealed a microdeletion carrying the CREBBP gene. Through this work, we emphasize the importance of clinical expertise in the diagnosis, management and genetic counseling in Rubinstein Taybi syndrome.
Keywords: Rubinstein Taybi syndrome; CREBBP gene; mutation; Moroccan.
Brucellosis and its associated risk factors to humans and domestic ruminants in Kagera Ecosystem, Tanzania
Authors: Bosco Ntirandekura Jean , Eliaimringi Matemba Lucas , Iddi Kimera Sharadhuli , Bwayla Muma John , Daniel Karimuribo Esron 
Published: August 2021

Background: Brucellosis is an important disease for both veterinary and public health. A study was conducted to under- stand the seroprevalence of brucellosis and its associated risk factors in pastoral areas of Kagera, Tanzania. Methods: Sera from 156 patients with malaria-like symptoms were analyzed using the commercial rapid agglutination test (specific for B.abortus and B.melitensis detection) and Fluorescence Polarization Assay (FPA). Sera from 426 cattle, 206 goats and 197 sheep were analyzed using Rose Bengal Plate (RBPT) and Competitive ELISA (c-ELISA) tests. Results: In humans, overall brucellosis, B. abortus, and B. melitensis sero-prevalences were 7.7% (95%CI: 3.8-12.2%), 1.9% (95% CI: 0.4-4.5%), and 5.8 % (95%CI: 2.6-10.6%), respectively. At animal level, seropositivity was 5.9% (95%CI: 4.0-8.6%), 2.5% (95%CI: 0.8-5.7%) and 0.5% (95%CI: 0.01-2.8%) in cattle, goats and sheep, respectively. At herd level, seropositivity was 18.2% (95%CI: 12.0-25.8%) in cattle and 6.9% (95%CI: 2.2-15.3%) in small ruminants. Brucellosis was associated with assisting in parturition without wearing protective gears (OR= 5.6; p= 0.02) in humans, herds of 50-200 animals (OR= 4.2, p= 0.01) and cattle (OR=3.5; p=0.01). The knowledge of brucellosis among pastoralists (OR=0.1; p<0.01) was a protective factor. Conclusion: Brucella infections could be occurring in pastoralists and domestic ruminants in Kagera. Community health education is necessary for the control of brucellosis in Tanzania.
Keywords: Brucellosis; pastoralists; risk factors; Tanzania.
Ecotoxicological assessment of commercial glyphosate-based di-ammonium salt formulation on haematological indices of Clarias gariepinus
Authors: Ella A.B. , Azua E.T. , Aguoru C.U. , Onekutu A.A. , Ella F.A. 
Published: August 2021

The study evaluated sub-lethal toxicity of commercial glyphosate-based Di-ammonium formulation commonly known as ‘Touchdown’ on haematological profile of Clarias gariepinus juveniles. A total of two hundred catfish juveniles of mean weight 31.07±1.23g and mean length 19.50 ± 0.50cm were exposed to different concentrations of Di-ammonium formulation (1.26, 2.52, 5.03 and 0.00 mg/l) for eight (8) weeks. Each concentration was treated in triplicate using a static bioassay system. Physico-chemical parameters of the test water were monitored throughout the study period. Results showed that haematocrit (HCT), red blood cell (RBC), haemoglobin (HGB), Mean Corpuscular Volume (MCV), Mean Corpuscular Haemoglobin (MCH), Mean Corpuscular Haemoglobin Concentration (MCHC) and Platelets (PLT) counts decreased significantly (p<0.05) with increase in concentrations of Touchdown. Whereas, white blood cell (WBC) counts increased significantly (p<0.05) with increasing concentrations of the glyphosate-based formulation. Water temperature, pH, Total dissolved solid (TDS) and electrical conductivity (EC) increased linearly with increasing concentration, while dissolved oxygen content decreased significantly (p<0.05). The results from this study indicated that Di-ammonium glyphosate–based formulation had negative consequences on the blood of treated fish. In view of this, the application of Di-ammonium glyphosate-based formulation should be done in a sustainable manner with proper regulations.
Influence of processing methods on quality and stability of silver catfish (Chrysichthys nigrodigitatus)
Authors: Effiong M.U. , Michael M.E. 
Published: August 2021

The study assessed different processing methods (freezing, smoking, oven drying and boiling) on fillet nutritional quality and stability of silver catfish. All analyses followed standard procedure. Results of proximate composition showed significant increase (p< 0.05) in protein (51.98%), lipid (11.65%), ash (3.82%) and calorie (432.57 Kcal/g) contents in the dried fillets. Thus, these variable increased with increasing processing temperature. On the other hand, moisture and carbohydrate contents decreased with temperature. The stability indices showed significant differences (p < 0.05) in free fatty acid of frozen (0.21%), boiled (0.8%), smoked (2.96%) and dried (9.87%). The lowest calcium (39.7%) was recorded in the frozen sample while the highest level (53.12%) in oven dried. Thiobarbituric acid decreased with increase in processing temperature with the highest level (27.14 mg MDA/100g) observed in the frozen fillet while the least value (2.08 mg MDA/100g) was obtained in the boiled samples. Calcium and phosphorus contents did not show any particular trend. The smoked fish fillet had the highest fibre content of 2.6%. Total volatile base nitrogen was generally low in all treatments. Inference from this study revealed that differently processed Chrysichthys nigrodigitatus was a rich source of fatty acids and essential minerals being very vital to the diet of humans.
Studies on fecundity and spawning of blue crab (Callinectes Amnicola) in Cross River Estuary
Authors: Otoyo J. , Ameh S.M. , Achema O.K. 
Published: August 2021

Studies on identification of species, fecundity and development stages of blue crab, (Callinectes amnicola) were investigated at the Cross River Estuary between the months of December, 2018 and May, 2019. The parameters used for the study were gonadosomatic and hepatosomatic indices, egg diameter and counts. All measurements followed standard procedures. Gonadosomatic index was at its peak in December (88.9) and April (83.3). This coincided with the peak in egg size suggesting that spawning occurred twice within the period.The low values of hepatosomatic index around December (1.20) and April (0.45) added to confirm the spawning in these months. The species spawns at a mean egg size of 17.55mm. The dimension of crabs examined were in the following range: carapace length 40.0 to 70.0mm, carapace width, 5.0 to 60mm and body weight 40.0 to 140.0g. The relationship between fecundity and weight of crab was significant (r = 0.295: p<0.05), indicating positive correlation. Absolute fecundity of the species ranged between 1,375 and 225,132 eggs. The smallest crab with egg had a carapace width of 39.5mm and weight of 46.9g. Spawning at this size seems to be a strategy adopted by the species to cope with exploitation pressure. The result of this study revealed that C. amnicola had a high reproductive potentials. Hence, adequate regulation is required to enhance sustainability of the species in the Cross River Estuary.
Enrichment of captive environment for effective animal reproduction
Authors: Oyeleye O.O. 
Published: August 2021

Animals are kept in captivity for the purpose of recreation, experimentation, rearing, reproduction, conservation, research etc. This study reviews how adequate welfare must be provided for the animals in captivity so that they will be able to perform to their maximum capabilities. It is imperative that the animals should not suffer because of the conditions they have been subjected to. The captive environment must be adequately enriched to foster effective reproduction that will sustain the population of the endangered species. This review has identified some of the problems faced by the captive animals and how to minimize these challenges. It is mandatory that before any animal is confined, adequate provision for its welfare must be provided to reduce any form of physiological or psychological stress.
Nilai Budaya dari Ritual Perkawinan Massarak sebagai Materi Pendidikan Keluarga Kristen di Mamasa
Authors: Sidu Masdariani , Rumbi Frans Paillin 
Published: August 2021

Generally, Christian education focuses on biblical preaching and church tradition. This method is correct, but it doesn't close the way for other approaches. Christian education can use different strategies to teach the Christian faith. This research focuses on the massarak marriage ritual. The researcher wants to find the cultural values contained in it. The focus of this research is the Mamasa people who live in the Nosu District, Kabupaten Mamasa. Furthermore, the researcher wants to see it from the aspect of Christian education. The authors used qualitative methods to answer the research objectives, namely data collected from observations and interviews, then the data were presented descriptively. The results showed positive values in massarak rituals, namely respect, independence, love, and solidarity. These values can be used in Christian education, of course, in dialogue with the Christian faith. AbstrakUmumnya pendidikan Kristen fokus pada pemberitaan alkitabiah dan tradisi gereja. Cara ini benar, tetapi bukan berarti menutup jalan atau akhir bagi pendekatan lain. Pendidikan Kristen dapat menggunakan pendekatan lain untuk mengajarkan iman Kristen. Penelitian ini menyoroti ritual perkawinan massarak, peneliti ingin menemukan nilai-nilai budaya yang terkandung di dalamnya. Lokus penelitian ini ialah orang Mamasa yang tinggal di Kecamatan Nosu, Kab. Mamasa. Selanjutnya peneliti hendak melihatnya dari aspek pendidikan Kristen. Penulis menggunakan metode penelitian kualitatif untuk menjawab tujuan penelitian, di mana data dikumpulkan dari hasil observasi dan wawancara, selanjutnya data disajikan secara deskriptif. Hasil penelitian memperlihatkan nilai-nilai positif dalam ritual massarak yakni penghormatan, kemandirian, kasih dan solidaritas. Nilai-nilai tersebut dapat digunakan dalam pendidikan Kristen, tentu saja dalam dialog dengan iman Kristen.
The Theology of a Jealous God: A Study of Exodus 20:3-5 and a Jealous God
Authors: Marpay Brian , Giawa Nasokhili 
Published: August 2021

Jealousy is one of the characteristics of God, which is essential in His divinity. It's just that some take it casually and even tend to see that jealousy like this is not too severe. This is why this article was written to ascertain the extent to which God's jealousy is essential to be studied by God's people and its application from the perspective of the responsibility of faith in Him. It should be understood that the Bible shows this nature of God, that He is a jealous God. However, this jealousy of God is certainly not as heavy as humans' jealousy in general. God was jealous of the acts of disloyalty made by humans against Him, namely deliberately rebelling against and worshiping other gods. God has the right to be jealous because of the disobedience of His chosen people, who have violated the content of His eternal covenant. Therefore, this article is written using a biblical-theological approach, namely trying to understand the purpose of God's jealousy as contained in Exodus 20: 3-5 by paying attention to the text and context and then analyzing it to find its value, relevance, and application in daily life. In the end, this article shows that the background behind God's jealousy is, firstly, there is a broken promise, second is the existence of a double attitude (because of a special relationship before), and the third is that there is an attitude of relationship dissatisfaction that causes jealousy, namely adultery with other gods. ABSTRAKSikap cemburu merupakan salah satu sifat khas Allah yang esensial dalam keilahian-Nya. Hanya saja, ada yang menanggapinya secara biasa saja bahkan cenderung melihat bahwa sifat cemburu seperti ini tidak terlalu serius. Inilah yang menyebabkan artikel ini ditulis untuk memastikan sejauh mana kecemburuan Tuhan penting dipelajari oleh umat Tuhan dan aplikasinya dari perspektif tanggung jawab iman kepada-Nya. Perlu dipahami bahwa Alkitab menunjukkan sifat Allah ini, bahwa Ia adalah Allah yang cemburu. Namun, kecemburuan Allah ini tentu tidak sama bobotnya dengan kecemburuan yang biasa dilakukan manusia pada umumnya. Sejujurnya Tuhan Allah sangat cemburu dengan tindakan ketidaksetiaan yang dibuat oleh manusia terhadap-Nya yaitu dengan sengaja memberontak dan menyembah allah lain. Allah berhak cemburu karena ketidak-taatan umat pilihan-Nya yang telah melanggar konten kovenan-Nya yang kekal. Karena itu, artikel ini ditulis menggunakan pendekatan teologi biblika yaitu berupaya memahami maksud kecemburuan Allah sebagaimana dimuat di dalam Kel. 20:3-5 dengan memperhatikan teks dan konteks lalu menganalisis untuk menemukan nilai, relevansi, dan aplikasinya dalam kehidupan sehari-hari. Pada akhirnya artikel ini memperlihatkan bahwa yang menjadi latar belakang di balik kecemburuan Allah ialah, pertama adanya janji yang diingkari, kedua ialah adanya sebuah sikap menduakan (karena adanya hubungan spesial sebelumnya), dan yang ketiga ialah adanya sikap ketidakpuasan relasi yang menimbulkan kecemburuan yaitu perzinahan dengan allah-allah lain.
Human Suffering and Theological Construction of Suffering
Authors: Zaluchu Sonny Eli 
Published: August 2021

Suffering, as a natural part of life, will be burdensome and burdensome when we respond in the wrong way. Therefore, it is necessary to have a theological construction so that humans can survive and pass through their sufferings victoriously. This paper aims to build a theological response to human suffering by proposing the presence of a theology of suffering. It can be concluded that through the theology of suffering, suffering humans can accept suffering as God's sovereignty. This theology also builds on the understanding that the way of suffering can identify God. The suffering experienced by humans does not come immediately because it has a unique purpose for everyone. It is also found that in the theology of suffering, God suffered through the death of His Son on the Cross for the benefit of humanity. This paper is written entirely with an analytic approach by relying on various theories and interpretations of Bible verses through in-depth literature studies ABSTRAK: Penderitaan sebagai bagian alami kehidupan, akan menjadi sesesuatu yang membebani dan menjerumuskan ketika ditanggapi dengan cara yang salah. Oleh sebab itu, diperlukan kehadiran sebuah konstuksi teologis agar manusia dapat bertahan dan melewati penderitaanya dengan kemenangan. Paper ini bertujuan untuk membangun tanggap teologis terhadap penderitaan manusia dengan mengusulkan kehadiran teologi penderitaan. Disimpulkan bahwa melalui teologi penderitaan, manusia yang menderita dapat menerima penderitaan sebagai sebuah kedaulatan Tuhan. Teologi ini juga membangun pengertian bahwa Allah dapat dikenali melalui jalan penderitaan. Penderitaan yang dialami manusia tidak hadir serta merta karena memiliki tujuan khas bagi setiap orang. Juga ditemukan bahwa di dalam sebuah teologi penderitaan, Allah ikut menderita melalui kematian anak-Nya di atas Salib untuk kepentingan manusia. Paper ini sepenuhnya ditulis dengan pendekatan analitik dengan mengandalkan berbagai teori dan tafsiran ayat-ayat Alkitab melalui pendalaman kajian pustaka.
God's Consistency Which Is Promised Through a New-Normal-Era Health Protocol in Order to Preserve The Essence Of Humanity
Authors: Napitupulu Lister 
Published: August 2021

Health anthropology has experienced a tremendous new revolution, started from last year, 2020. A New-normal Health Protocol formulation was put in place to deal with the Covid-19 pandemic outbreak. Natural and social media communities complained about humanity and human rights degradation, raising calls for resistance due to imposed global restrictive regulations. God in Christian theology is a God who promises to His human creation that He will be with and help humans to have a happy and prosperous life. By doing literature research, this paper tries to explore whether the Covid-19 management guidelines are by the consistency of covenant God in restoring the essence of humanity. How is the relation between two paradigms of society, from God’s side and human’s side, through the Health Protocol intervention? The finding is that God, who created perfect human beings, keeps all the processes of his life to become the people of His eternal kingdom. Society and individuals should be able to respond to global regulations as support for faith and perspective to continue to live and fill the days ahead with the maximum quality of life as prime human beings. ABSTRAK: Antropologi kesehatan mengalami suatu revolusi baru yang dahsyat, dimulai dari tahun lalu, 2020. Sebuah rumusan Protokol Kesehatan New-normal diberlakukan untuk menangani wabah pandemi Covid-19. Masyarakat nyata dan media sosial mengeluhkan perasaan degradasi kemanusiaan dan hak asasi manusia bahkan mencuatkan seruan perlawanan karena peraturan pembatasan global yang dipaksakan. Allah dalam teologi Kristen adalah Allah yang berjanji kepada manusia ciptaan-Nya bahwa Ia akan menyertai dan menolong manusia untuk mendapatkan kehidupan yang sejahtera dan berbahagia. Dengan melakukan sebuah literature-research tulisan ini mencoba menelusuri apakah pedoman tatalaksana Covid-19 ini sesuai dengan konsistensi Allah yang berjanji dalam hal memulihkan esensi kemanusiaan. Bagaimanakah hubungan antara dua paradigma tentang kemanusiaan dari sisi Allah dan manusia melalui intervensi Protokol Kesehatan. Temuan bahwa Allah yang menciptakan manusia yang sempurna menjaga sepanjang proses kehidupannya agar menjadi umat kerajaan kekal-Nya. Seyogyanya masyarakat dan individu dapat menyikapi peraturan global sebagai penopang iman dan cara pandang sehingga tetap dapat menjalani dan mengisi hari-hari ke depan dengan kualitas hidup maksimal sebagai manusia prima.