bluuebunny/crossref_metadata_2025_split huggingface dataset:
Nucleoside antibiochemotherapy repressed the growth, chemoresistance, survival, and metastatic potentials of castration-resistant prostate cancer cells
Authors: Oseni Saheed Oluwasina , Laguer Genesis Acosta , Ambrin Faika , Philemy Magdalah , Betty Javoncia , Kumi-Diaka James 
Published: August 2021

ABSTRACTThere is currently no definitive cure for metastatic castration-resistant prostate cancer (mCRPC), therefore justifying the incessant need for more investigative studies to either repurpose old drugs or identify novel and effective therapeutics. In this study, we investigated the possible anticancer effects of two nucleoside antibiotics: puromycin and blasticidin. We hypothesized that the two antibiotics alone or combined with other drugs will inhibit prostate cancer (PCa) cell proliferation and metastasis and induce cell death via apoptosis. mCRPC cell lines (PC3 and DU145) with different p53-gene statuses were cultured and seeded in 96 well-plates, and thereafter treated with varying concentrations of puromycin and blasticidin (1 ng/mL - 100 μg/mL) for 24 - 48 hours. Resazurin reduction and/or MTT assays were done to evaluate the treatment-induced effects on mCRPC cell viability and proliferation. The colony-forming assay measured the cell survival rate following treatment nucleoside antibiotics while scratch migration assay and dual-fluorescent microscopy assessed the effects on metastatic potential and cell death, respectively. The two antibiotics were combined with either paclitaxel, docetaxel, or cabazitaxel to check for synergism. Our results indicate that both antibiotics exhibit dose- and time-dependent anticancer effects on growth, survival, and metastasis of mCRPCs. PC3 cells were significantly more susceptible to both antibiotics compared to DU145 cells. Both cell lines were more susceptible to puromycin compared to blasticidin. Synergism was observed when each antibiotic compound was combined with any of the three taxanes. In conclusion, we have demonstrated that both puromycin and blasticidin could be explored for the treatment of mCRPC.GRAPHICAL ABSTRACT
Quantitative live-cell imaging of GPCR downstream signaling dynamics
Authors: Tany Ryosuke , Goto Yuhei , Kondo Yohei , Aoki Kazuhiro 
Published: August 2021

AbstractG-protein-coupled receptors (GPCRs) play an important role in sensing various extracellular stimuli, such as neurotransmitters, hormones, and tastants, and transducing the input information into the cell. While the human genome encodes more than 800 GPCR genes, only four Gα-proteins (Gαs, Gαi/o, Gαq/11, and Gα12/13) are known to couple with GPCRs. It remains unclear how such divergent GPCR information is translated into the downstream G-protein signaling dynamics. To answer this question, we report a live-cell fluorescence imaging system for monitoring GPCR downstream signaling dynamics at the single-cell level. Genetically encoded biosensors for cAMP, Ca2+, RhoA, and ERK were selected as markers for GPCR downstream signaling, and were stably expressed in HeLa cells. GPCR was further transiently overexpressed in the cells. As a proof-of-concept, we visualized GPCR signaling dynamics of 5 dopamine receptors and 12 serotonin receptors, and found heterogeneity between GPCRs and between cells. Even when the same Gα proteins were known to be coupled, the patterns of dynamics in GPCR downstream signaling, including the signal strength and duration, were substantially distinct among GPCRs. These results suggest the importance of dynamical encoding in GPCR signaling.
Emergence and Enhancement of Ultrasensitivity through Posttranslational Modulation of Protein Stability
Authors: Kumbale Carla M. , Voit Eberhard O. , Zhang Qiang 
Published: August 2021

AbstractSignal amplification converts a linear input to a steeply sigmoid output and is central to cellular functions. One canonical signal amplifying motif is zero-order ultrasensitivity through the posttranslational modification (PTM) cycle signaling proteins. The functionality of this signaling motif has been examined conventionally by supposing that the total amount of the protein substrates remains constant. However, covalent modification of signaling proteins often results in changes in their stability, which affects the abundance of the protein substrates. Here we use a mathematical model to explore the signal amplification properties in such scenarios. Our simulations indicate that PTM-induced protein stabilization brings the enzymes closer to saturation, and as a result, ultrasensitivity may emerge or is greatly enhanced, with a steeper sigmoidal response of higher magnitude and generally longer response time. In cases where PTM destabilizes the protein, ultrasensitivity can be regained through changes in the activities of the involved enzymes or from increased protein synthesis. Interestingly, ultrasensitivity is not limited to modified or unmodified protein substrates; the total protein substrate can also exhibit ultrasensitivity. It is conceivable that cells use inducible protein stabilization as a way to boost signal amplification while saving energy by keeping the protein substrate at low basal conditions.
Flexibility and job stress in the accounting profession
Authors: Mnif Yosra , Rebai Emna 
Published: August 2021

Purpose
The purpose of this study is to examine the effects of flexibility as a coping mechanism for reducing the negative impact of job stress on the accounting profession.

Design/methodology/approach
Data were collected using a survey questionnaire sent by mail to accounting professionals in Tunisia. A total of 200 responses were examined using a structural equations modeling procedure.

Findings
The results indicate that flexibility attenuates the negative effects of job stress by improving job performance and job satisfaction among accounting professionals. Also, flexibility has a significant and positive association with psychological well-being. The results demonstrate that role ambiguity and overload have a positive influence on job burnout, which supports the argument that role stressors are antecedents of burnout in the accounting profession.

Practical implications
This study’s results help the accounting firms to reduce job stress by offering work flexibility to employees. Also, the results have significant implications for accounting professionals who are concerned with the consequences of job stress and the coping mechanisms that can diminish these consequences.

Originality/value
This study contributes to the behavioral accounting literature by examining flexibility as a coping strategy within the job stress model in an emerging country (Tunisia).
Key competencies for critical infrastructure cyber-security: a systematic literature review
Authors: Chowdhury Nabin , Gkioulos Vasileios 
Published: August 2021

Purpose
The purpose of this paper can be encapsulated in the following points: identify the research papers published on the topic: competencies and skills necessary for critical infrastructure (CI) cyber-security (CS) protection; determine main focus areas within the identified literature and evaluate the dependency or lack thereof between them: make recommendations for future research.

Design/methodology/approach
This study is based on a systematic literature review conducted to identify scientific papers discussing and evaluating competencies, skills and essential attributes needed by the CI workforce for CS and preparedness to attacks and incidents.

Findings
After a comparative analysis of the articles reviewed in this study, a variety of skills and competencies was found to be necessary for CS assurance in CIs. These skills have been grouped into four categories, namely, technical, managerial, implementation and soft skills. Nonetheless, there is still a lack of agreement on which skills are the most critical and further research should be conducted on the relation between specific soft skills and CS assurance.

Research limitations/implications
Investigation of which skills are required by industry for specific CS roles, by conducting interviews and sending questionnaire\surveys, would allow consolidating whether literature and industry requirements are equivalent.

Practical implications
Findings from this literature review suggest that more effort should be taken to conciliate current CS curricula in academia with the skills and competencies required for CS roles in the industry.

Originality/value
This study provides a previously lacking current mapping and review of literature discussing skills and competencies evidenced as critical for CS assurance for CI. The findings of this research are useful for the development of comprehensive solutions for CS awareness and training.
Modification optimization-based fatigue life analysis and improvement of EMU gear
Authors: Li Yong-Hua , Zhang Chi , Yin Hao , Cao Yang , Bai Xiaoning 
Published: August 2021

PurposeThis paper proposes an improved fatigue life analysis method for optimal design of electric multiple units (EMU) gear, which aims at defects of traditional Miner fatigue cumulative damage theory.Design/methodology/approachA fatigue life analysis method by modifying S–N curve and considering material difference is presented, which improves the fatigue life of EMU gear based on shape modification optimization. A corrected method for stress amplitude, average stress and S–N curve is proposed, which considers low stress cycle, material difference and other factors. The fatigue life prediction of EMU gear is carried out by corrected S–N curve and transient dynamic analysis. Moreover, the gear modification technology combined with intelligent optimization method is adopted to investigate the approach of fatigue life analysis and improvement.FindingsThe results show that it is more corresponded to engineering practice by using the improved fatigue life analysis method than the traditional method. The function of stress and modification amount established by response surface method meets the requirement of precision. The fatigue life of EMU gear based on the intelligent algorithm for seeking the optimal modification amount is significantly improved compared with that before the modification.Originality/valueThe traditional fatigue life analysis method does not consider the influence of working condition and material. The life prediction results by using the method proposed in this paper are more accurate and ensure the safety of the people in the EMU. At the same time, the combination of intelligent algorithm and gear modification can improve the fatigue life of gear on the basis of accurate prediction, which is of great significance to the portability of EMU maintenance.
Salesperson attributes that influence consumer perceptions of sales interactions
Authors: Fergurson J. Ricky , Gironda John T. , Petrescu Maria 
Published: August 2021

Purpose
This paper aims to examine how modern, digital era customers in a business-to-consumer (B2C) setting prioritize salesperson-customer orientation attributes when evaluating their expectations regarding interactions with salespeople, as well as their impact on positive and negative word-of-mouth. In addition, the research further investigates which negative salesperson attributes have an impact on overall customer experience and satisfaction.

Design/methodology/approach
Role theory and expectancy-disconfirmation theory form the theoretical foundation for two mixed-method studies. Study 1 is an exploratory content analysis of online consumer reviews and social media word-of-mouth related to consumer experiences with salespeople. Study 2 is a three-round Delphi study investigating which salesperson orientation attributes are most important to the customer in B2C interactions.

Findings
The results uncover which salesperson customer orientation attributes are essential for modern consumers and how they differ as a function of context (retail, direct-selling and follow-up) and how they contribute to the generation of digital word-of-mouth.

Originality/value
This paper expands B2C sales interaction literature by exploring the need for differing sales attributes based on the differential function of the shopping environment.
The anatomy of a rivalry: the role of resentment in the development of brand attitudes
Authors: Alvarado-Karste Diego , Kidwell Blair 
Published: August 2021

Purpose
This study aims to demonstrate that feelings of resentment, fueled by perceptions of injustice, underlie the formation of rivalries. Further, this study analyzes how consumers evaluate the two brands that participate in a rivalry relationship.

Design/methodology/approach
The research uses four experiments. Study 1 uses two conditions to test whether injustice predicts inter-personal rivalries through resentment. Study 2 uses a one-factor design with three levels (resentment vs contempt vs control) to examine the underlying mechanism of resentment on the formation of a rivalry. Study 3 analyzes the effect of brand rivalries on consumers’ brand attitudes. Study 4 uses a 2 (Temporal-focus: past vs future) × 2 (competitive relationship: resentment vs control) between-subjects experimental design, to test the moderating effects of temporal-focus on consumer brand rivalry perceptions. This experiment replicates the effects of brand rivalries on consumer brand attitudes.

Findings
Rivalries have an essential emotional component – resentment – that is fueled by injustice and leads consumers to form more favorable attitudes toward the brand that consumers perceive is treated unfairly (target brand) and more unfavorable attitudes toward the brand that is perceived to treat the other brand unfairly (the rival brand). A future-focused mindset attenuates consumer perceptions of brand rivalries, whereas a past-focused mindset enhances these effects.

Originality/value
Prior research has failed to identify the emotional components of rivalries and their effects on consumer choices. To the best of the authors’ knowledge, this is the first study that reveals how attitudes change when consumers are exposed to a brand rivalry.
Transmission of negative brand-relevant content on social media
Authors: Powell Ashleigh , Stavros Constantino , Dobele Angela 
Published: August 2021

Purpose
Understanding how to predict and manage the spread of negative brand-relevant content is of critical concern to marketers. This paper aims to contribute to this understanding by building on existing anti-branding, brand hate and word-of-mouth literature to explore the factors that lead individuals to engage in the transmission of negative brand-relevant information via social media.

Design/methodology/approach
A two-phase exploratory design was used. The first stage involved an analysis of negative transmission via comments left on news and brand posts. The second phase of the research involved a series of 13 depth interviews with frequent social media users about their negative brand-relevant transmission behavior to add richness and depth to the findings from the passive observation in the first phase of the research.

Findings
The first phase of the research demonstrated that negative transmission can be both brand-related (e.g. driven by-product or service failure or corporate irresponsibility) and consumer-related (e.g. driven by self or social motives). The second phase of the research clarified that negative transmission often occurs in the absence of brand hate, particularly when it can be used as a covert method of self-enhancement for the transmitter via downward social comparisons.

Originality/value
Negative transmission as a form of anti-branding that is more strongly self-related (as opposed to brand) is established, progressing understanding and applications of contemporary media channels. Implications, including how brand-generated controversy and consumer reinforcement can be used to manage negative transmission, are offered.
Servicing through digital interactions andwell-being in virtual communities
Authors: Chou Cindy Yunhsin , Leo Wei Wei Cheryl , Chen Tom 
Published: August 2021

Purpose
Applying social exchange theory as the theoretical basis, this paper aims to examine the impacts of two forms of digital social interaction on social well-being and helping behavior of customers: moderator–customer interaction quality and customer–customer social support. Furthermore, this paper investigates customer exchange ideology as a moderator of these impacts.

Design/methodology/approach
This paper adopted a purposive sampling method for survey materials sent to customers of firm-hosted virtual communities (FHVCs) using a consumer panel service company. The self-administered survey was developed from existing scales, and 265 usable responses were obtained.

Findings
Both forms of digital social interaction within FHVCs positively impact social well-being, which in turn positively influences helping behavior in the community. Social well-being is decomposed into social integration and social contribution, and each partially mediates the impact of customer–customer social support and moderator–customer interaction quality on helping behavior. This finding provides greater explanatory power for the role that digital social interactions have in predicting customer helping behavior in an FHVC. In addition, an exchange ideology positively moderates the impact of customer–customer social support on helping behavior via social integration.

Originality/value
This paper demonstrates that resource exchange dynamics occur digitally within FHVCs, which then affect social well-being and helping behaviors in customers. From a practical point of view, this study indicates the potential that digital interactions have in generating social and economic value through helping behaviors.
Training transfer to the workplace: a study of corporate English language training in hospitals
Authors: Ha Huong , Vanaphuti Vanvela 
Published: August 2021

Purpose
This study aims to examine factors that affect the effectiveness of transfer of English language training to the workplace. It investigates the effect of trainee characteristics, training design and work climate on the training transfer to job performance in hospitals in Thailand.

Design/methodology/approach
The quantitative method was adopted in this study. The conceptual framework was developed from Lim and Morris’ three-factor model, namely, trainee characteristics, training design and work climate. A total of 378 valid responses from staff of seven hospitals in Thailand were collected from surveys.

Findings
The findings suggest that training transfer depends more on trainees’ psychological state (affective response and self-efficacy) than extrinsic factors. Nearly every aspect of training design could affect training transfer, except trainer effectiveness. This could be related to the particular context of training and the trainees’ prior experience. Work climate factors had the strongest overall effect, with peer and supervisor feedback, compensation and incentives and transfer opportunities being significant.

Originality/value
This study proposes that training design and work climate-related factors deserve more attention than what they have received previously. This study is significant because of the limited empirical evidence for English training transfer outcomes, and the under-examined role of English as a lingua franca in the business world. The findings can help organisations refine training designs and adjust the work environment to improve training outcomes.
Circulating tumor cells (CTCs)/circulating tumor endothelial cells (CTECs) and their subtypes in small cell lung cancer: Predictors for response and prognosis
Authors: Zhu Hao‐Hua , Liu Yu‐Tao , Feng Yu , Zhang Li‐Na , Zhang Tong‐Mei , Dong Gui‐Lan , Xing Pu‐Yuan , Wang Hong‐Yu , Shi Yuan‐Kai , Hu Xing‐Sheng 
Published: August 2021

AbstractBackgroundThe aim of the study was to define the clinical significance of circulating tumor cells (CTCs)/circulating tumor endothelial cells (CTECs) and their subtypes in small cell lung cancer (SCLC) patients.MethodsCTCs/CTECs and their subtypes were determined using SE‐iFISH technology in 33 SCLC patients before initial treatment (B1), after two cycles of chemotherapy (B2), at the completion of chemotherapy (B3), and disease progression (B4). The correlations with clinical characteristics, progression‐free survival (PFS), and overall survival (OS) were analyzed.ResultsCTCs and CTECs were detected in 96.6% and 65.5% of patients, respectively. Patients had higher levels of CTCs compared with CTECs in circulation (p < 0.05). Extensive‐stage SCLC patients tended to have higher CTEC counts (p = 0.035), and the detection of CTC‐white blood cell (CTC‐WBC) clusters was associated with a worse response to treatment (p = 0.030). Patients with CTC‐WBC clusters at B1 (17.3 vs. 22.6 months, p = 0.041) and B2 (19.9 vs. 25.2 months, p = 0.018) had significantly shorter OS than those with no detection. Additionally, their presence was revealed as independent predictors for a worse OS in multivariable analyses (B1: HR 9.3, 95% CI: 1.4–48, p = 0.0079; B2: HR 4.4, 95% CI: 1.1–18, p = 0.041). A high CTC level at B4 was an adverse prognostic factor for SCLC patients (PFS: 8.7 vs. 22.5 months, p = 0.0026; OS: 19 months vs. not reached, p = 0.0086). CTC clusters and CTECs also showed prognostic values.ConclusionsThe presence of CTC‐WBC clusters at baseline and after two‐cycle chemotherapy and the total CTC counts at the completion of chemotherapy are strong predictors for the prognostic survival of SCLC patients receiving first‐line treatment.
All the President's Justices? The Impact of Presidential Copartisanship on Supreme Court Job Approval
Authors: Bartels Brandon L. , Kramon Eric 
Published: August 2021

AbstractSupreme Court job approval is sensibly connected to its decisions, particularly salient ones. We fill a gap in the literature by theorizing—via a presidential appointment mechanism—how partisan alignment with the incumbent president (presidential copartisanship) influences Supreme Court job approval. Analysis of data from 1986 to 2019 (supplemented by longer‐term confidence data) shows that a president's copartisans are significantly more approving of the Court than outpartisans. Analysis of the American Panel Survey surrounding high‐salience events during the transition from Obama to Trump shows that Republicans, who significantly increase in Court approval following Trump's election victory, are anticipatory of Trump's prospects of changing the Court. Democrats, whose approval significantly declines only after Justice Gorsuch's confirmation, are not anticipatory but reactive to the president's confirmed appointee. Our findings generate new evidence of how the president structures public opinion toward the Court, which has important implications for judicial independence and legitimacy.