bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comparative transcriptional analysis of the satellite glial cell injury response
Authors: Jager Sara Elgaard , Pallesen Lone Tjener , Lin Lin , Izzi Francesca , Pinheiro Alana Miranda , Villa-Hernandez Sara , Cesare Paolo , Vaegter Christian Bjerggaard , Denk Franziska 
Published: November 2021

AbstractSatellite glial cells (SGCs) tightly surround and support primary sensory neurons in the peripheral nervous system and are increasingly recognized for their involvement in the development of neuropathic pain following nerve injury. The SGCs are difficult to investigate due to their flattened shape and tight physical connection to neurons in vivo and their rapid changes in phenotype and protein expression when cultured in vitro. Consequently, several aspects of SGC function under normal conditions as well as after a nerve injury remain to be explored. The recent advance in single cell RNAseq technologies has enabled a new approach to investigate SGCs. Here we publish a dataset from mice subjected to sciatic nerve injury as well as a dataset from dorsal root ganglia cells after 3 days in culture. We use a meta-analysis approach to compare the injury response with that in other published datasets and conclude that SGCs share a common signature following sciatic nerve crush and sciatic ligation, involving transcriptional regulation of cholesterol biosynthesis. We also observed a considerable transcriptional change when culturing SGCs, suggesting that some differentiate into a specialised in vitro state, while others start resembling Schwann cell-like precursors. The datasets are available via the Broad Institute Single Cell Portal.
Fine-grained, Nonlinear Image Registration of Live Cell Movies Reveals Spatiotemporal Organization of Diffuse Molecular Processes
Authors: Jiang Xuexia , Isogai Tadamoto , Chi Joseph , Danuser Gaudenz 
Published: November 2021

AbstractWe present an application of non-linear Image registration that allows spatiotemporal analysis of extremely noisy and diffuse molecular processes across the entire cell. To produce meaningful local tracking of the spatially coherent portion of diffuse protein dynamics, we improved upon existing non-linear image registration to compensate for cell movement and deformation. The registration relies on a subcellular fiducial marker, a cell motion mask, and a topological regularization that enforces diffeomorphism on the registration without significant loss of granularity. We demonstrate the potential of this approach in conjunction with stochastic time-series analysis through the discovery of distinct zones of coherent Profillin dynamics in symmetry-breaking U2OS cells. Further analysis of the resulting Profilin dynamics revealed strong relationships with the underlying actin organization. This study thus provides a framework for extracting functional interactions between cell morphodynamics, protein distributions, and signaling in cells undergoing continuous shape changes.Author SummaryBy adapting optical flow based nonlinear image registration we created a method specific for live cell movies that preserves the dynamics of a signal of interest by remapping using a separate measurable subcellular location fiducial. This is an extension as well on our lab’s previously published method of cell edge-based referencing of subcellular locations that was incapable of extracting interpretable subcellular time series more than a few microns away from the cell edge. We showed that our method overcomes this key limitation and allows sampling of subcellular time series from every subcellular location through our discovery of organized profilin dynamics in moving cell and that these profilin dynamics are related to actin dynamics due to their ability to bind growing actin structures likely through actin polymerizing factors. Most importantly, our method is applicable to discovering subcellular organization and coordination in a widely used form of live cell microscopy data that hitherto has been largely limited to anecdotal analysis.
Materials on Plant Leaf Surfaces are Deliquescent in a Range of Environments
Authors: Tredenick E. C. , Stuart-Williams H. , Enge T. G. 
Published: November 2021

AbstractMaterials on plant leaf surfaces that attract water impact penetration of foliar-applied agrochemicals, foliar water uptake, gas exchange and stomatal density. Few studies are available on the nature of these substances, and we quantify the hygroscopicity of these materials. Water vapor sorption experiments on twelve leaf washes of sample leaves were conducted and analyzed with inductively coupled plasma-optical emission spectroscopy and X-ray diffraction. All leaf surface materials studied were hygroscopic. Oils were found on the surface of the Eucalyptus studied. For mangroves that excrete salt to the leaf surfaces, significant sorption occurred at high humidity of a total of 316 mg (∼ 0.3 mL) over 6–10 leaves, and fitted a Guggenheim, Anderson, and de Böer sorption isotherm. Materials on the plant leaf surface can deliquesce and form an aqueous solution in a variety of environments where plants grow, including glasshouses and by the ocean, which is an important factor when considering plant-atmosphere relations.
Value cocreation in new service development: a process-based view of resource dependency
Authors: Wang Qiang , Oshri Ilan , Zhao Xiande 
Published: November 2021

Purpose
This study aims to examine value cocreation in terms of interfirm collaborations between service firms, their business customers and business partners at different stages of a new service development (NSD) process.

Design/methodology/approach
The study develops and tests hypotheses that examine the roles played by business customers and partners in NSD, assuming resource dependency of the focal firm during three NSD stages (ideation, development and deployment). Empirical data were collected from 200 NSD projects and structural equation modelling was used to test the hypotheses.

Findings
The results show that business customer collaboration has a positive effect on ideation performance and development performance, whereas business partner collaboration has a positive effect on deployment performance. These finding support the notion that the value cocreating roles of business customers and partners vary across NSD stages.

Research limitations/implications
Future research should focus on how business partners can be actively involved in the NSD process and how the interests of different parties can be safeguarded. The use of longitudinal data will allow a better examination of the process dynamics.

Practical implications
The study provides managerial implications for service managers in terms of acquiring and allocating resources needed from business customers and partners during different NSD stages.

Originality/value
The study contributes to the growing literature on value cocreation in NSD by empirically demonstrating the respective performance contributions of business customers and partners during different stages of NSD. Moreover, the results also shed light on interfirm collaboration mechanisms from the perspective of resource dependence theory.
Rethinking the strategic management of human resources: lessons learned from Covid-19 and the way forward in building resilience
Authors: Roumpi Dorothea 
Published: November 2021

PurposeNumerous empirical studies and meta-analyses have offered ample evidence for the relationship between the strategic management of human resources and a variety of important organizational outcomes, such as individual- and firm-level performance. The outbreak of the COVID-19 pandemic, however, steered the discussion away from the traditional performance-related outcomes of the strategic human resource management and brought to the fore the importance of resilience. The purpose of this paper is to identify areas in which strategic human resource management can help organizations to become more resilient.Design/methodology/approachOn the basis of a brief overview of the different phases of the COVID-19 pandemic and its impact on the management of human resources, this conceptual paper uses the Ability-Motivation-Opportunity framework as the backbone for the development of suggestions for the strategic management of employees to create and maintain organizational resilience.FindingsA series of actionable suggestions regarding the way forward in building organizational resilience through the effective and ethical management of employees are presented and discussed. Specifically, the importance of using flexibility-oriented human resource management systems is highlighted. This “flexibility-orientation” involves all functions of human resource management, such as selection, training and compensation.Originality/valueThe proposed suggestions can benefit organizations by offering actionable recommendations regarding the management of human resources by taking stock of lessons learned during the COVID-19 pandemic.
Opentable as a marketing strategy for modern Muslim funeral product in Indonesia
Authors: Prihatiningsih Witanti , Damayani Ninis Agustini , Suryana Asep , Perbawasari Susie 
Published: November 2021

Purpose
The Opentable strategy is considered a taboo because of its ability to take advantage of peoples’ grief. Therefore, this study aims to explore the Opentable (Exhibition) as a marketing strategy for modern Muslim funeral products, considered a luxury by most people in Indonesia.

Design/methodology/approach
This is a qualitative research with a phenomenological approach used to collect data from six Memorial Advisors (MAs) through interviews.

Findings
The result showed that the Opentable strategy was carried out to introduce the concept of a modern Muslim funeral home and its products. Furthermore, MA does not encourage sales during Opentable, rather, it uses the process to obtain complete data from potential customers.

Practical implications
This research serves as a guidance for MA and other similar professions to socialize products uncommon or considered taboo in society. It also provides a repertory of new marketing strategies, especially for targeted and uncommon products.

Originality/value
To the best of the knowledge, this is the first research to explore Opentable (Exhibition) as a marketing strategy for modern Muslim funeral products in Indonesia. Therefore, future research is needed on other marketing strategies.
Cultural differences in deliberate counterfeit purchase behavior
Authors: Sharma Piyush , Chan Ricky Y. K. , Davcik Nebojsa , Ueno Akiko 
Published: November 2021

PurposeThis paper explores the moderating effects of four personal cultural orientations or PCOs (independence, interdependence, risk aversion and ambiguity intolerance) on the relationships among counterfeit proneness, subjective norms, ethical judgments, product evaluation and purchase intentions for counterfeit products.Design/methodology/approachA field study with 840 consumers in Hong Kong using a self-administered structured questionnaire is used to test all the hypotheses.FindingConsumers with high (low) scores on interdependence (independence) show stronger positive effects of counterfeit proneness on subjective norms and its effects on the counterfeit evaluation and purchase intentions. In contrast, consumers with high (low) scores on independence (interdependence) show stronger positive effects of counterfeit proneness on ethical judgments and its effects on counterfeit evaluation and purchase intentions. Consumers with higher scores on risk aversion and ambiguity intolerance show negative moderating effects on most of the relationships in the unified conceptual framework.Research limitations/implicationsThe authors collected data in Hong Kong, which is predominantly Chinese in culture. Hence, future research in other parts of the world with more diverse cultural values would help test the validity and generalizability of the results.Practical implicationsThe findings would be useful for managers of genuine brands to learn more about the process that explains deliberate counterfeit purchase behavior.Originality/valueThe authors extend the unified conceptual framework for deliberate counterfeit purchase behavior by incorporating four PCOs to explore cultural differences in the socio-psychological decision-making process underlying this behavior.
Digital transformation: “jobocolypse” or empowerment?
Authors: Hizir Zeynep 
Published: November 2021

Purpose
The purpose of this paper is to analyse the impact of digital transformation on jobs and to assess whether or not the so-called Fourth Industrial Revolution will lead to mass redundancies. The piece also looks at the role human resources (HR) departments will have to play in the implementation of, and response to, digital transformation within the workplace.

Design/methodology/approach
This paper combines research based on media articles, a parliamentary report and wider digital transformation industry research. The author looks to explain what digital transformation is, deconstruct misconceptions around digital transformation, assess the true evidence-based impact of digital transformation on jobs and advise HR departments on the impact of digital transformation within the workplace.

Findings
This piece finds that there remains a considerable degree of misunderstanding and many misconceptions around digital transformation and that while digital transformation will lead to the loss of some jobs, it will also lead to the change of existing ones and creation of new ones. Furthermore, this piece finds that HR departments will play a crucial role in the implementation of digital transformation, but that they too will need to pivot and adjust to new workplace realities as a result.

Originality/value
Many HR departments face confusion and misconceptions around the impact of digital transformation on the workforce, and there also remains concern among many employers that technology will replace their jobs. This piece seeks to dispel the myths and paint a clearer picture to both HR departments and employees around the impact of digital transformation.
Successful treatment of tumor stage mycosis fungoides with total skin helical tomotherapy
Authors: Yonekura Kentaro , Ichiki Mamoru , Takeda Koichiro , Uchiyama Noriaki , Nishida Hirotoshi , Dokiya Takushi 
Published: November 2021

AbstractTotal skin electron beam therapy (TSEBT) is a treatment option for mycosis fungoides (MF). In Japan, it has been rarely performed because of the time required for each treatment, physical burden on patients, and difficulties in providing uniform dosimetry. In recent years, helical tomotherapy, an intensity‐modulated radiation therapy that applies helical computed tomography technology, has been used to treat cancer. Total skin helical tomotherapy (TSHT) has been suggested as a promising alternative to TSEBT for patients with MF, but there are few reports from Japan. We used TSHT to treat a 28‐year‐old Japanese woman with tumor stage MF. She achieved complete remission with TSHT (12 Gy in six fractions over 6 days) and remained in remission for 32 months without additional treatment. Treatment‐related grade 4 myelosuppression was observed, but resolved with blood transfusions and subcutaneous injection of granulocyte colony stimulating factor. Other adverse events were tolerable. Although careful attention should be paid to myelosuppression, TSHT might be a useful treatment option for MF.
Mobile assessment of decisions to drink in young adults: Examining the role of incentives and disincentives
Authors: Howe Lindy K. , Copeland Savanna , Fisher Lindsey , Farmer Eli , Nemes Luca , Finn Peter R. 
Published: November 2021

AbstractBackgroundResearch on decision‐making (DM) biases in persons with alcohol use disorder have largely relied on behavioral economic DM tasks, which do not assess the dynamic multitude of factors associated with real‐world decisions about drinking. The current study extends the literature on DM and alcohol use by using a mobile daily diary approach to investigate whether, and how, real‐world incentives and disincentives are associated with actual drinking decisions in college students.MethodsWe assessed current drinking and lifetime alcohol problems in 104 young adults (61.5% female, 84.5% White) who, for 14 days, used a mobile daily diary to respond to questions about drinking decisions from the day prior. Mobile prompts assessed daily data on the timing of drinking decisions, alcohol quantity initially decided to drink, quantity actually decided to drink, and the incentives that influenced drinking decisions.ResultsUsers of the app reported high usability, high compliance rates were observed, and incentive responses were reduced to three subtypes: alcohol, social/party, and mood. Daily mobile measures of drinking quantity were strongly correlated with self‐reported drinking 2 weeks prior to the daily assessment. Lifetime alcohol problems were positively related to the average quantity decided to drink per event, drinking more than initially decided, and higher levels of self‐reported drinking‐decision incentives. “Alcohol” and “social/party” incentives were positively related to the quantity the participant decided to drink at drinking events. Mood incentives and disincentives showed little to no significant relationships with drinking decisions.ConclusionsThe results suggest that mobile data collection can be useful for assessing aspects of real‐world drinking decisions and the influence of multiple drinking decision incentives.
Expression pattern of mitochondrial respiratory chain enzymes in skeletal muscle of patients with mitochondrial myopathy associated with the homoplasmic m.14674T>C variant
Authors: Roos Sara , Hedberg‐Oldfors Carola , Visuttijai Kittichate , Stein My , Kollberg Gittan , Elíasdóttir Ólöf , Lindberg Christopher , Darin Niklas , Oldfors Anders 
Published: November 2021

AbstractTwo homoplasmic variants in tRNAGlu (m.14674T>C/G) are associated with reversible infantile respiratory chain deficiency. This study sought to further characterize the expression of the individual mitochondrial respiratory chain complexes and to describe the natural history of the disease. Seven patients from four families with mitochondrial myopathy associated with the homoplasmic m.14674T>C variant were investigated. All patients underwent skeletal muscle biopsy and mtDNA sequencing. Whole‐genome sequencing was performed in one family. Western blot and immunohistochemical analyses were used to characterize the expression of the individual respiratory chain complexes. Patients presented with hypotonia and feeding difficulties within the first weeks or months of life, except for one patient who first showed symptoms at 4 years of age. Histopathological findings in muscle included lipid accumulation, numerous COX‐deficient fibers, and mitochondrial proliferation. Ultrastructural abnormalities included enlarged mitochondria with concentric cristae and dense mitochondrial matrix. The m.14674T>C variant in MT‐TE was identified in all patients. Immunohistochemistry and immunoblotting demonstrated pronounced deficiency of the complex I subunit NDUFB8. The expression of MTCO1, a complex IV subunit, was also decreased, but not to the same extent as NDUFB8. Longitudinal follow‐up data demonstrated that not all features of the disorder are entirely transient, that the disease may be progressive, and that signs and symptoms of myopathy may develop during childhood. This study sheds new light on the involvement of complex I in reversible infantile respiratory chain deficiency, it shows that the disorder may be progressive, and that myopathy can develop without an infantile episode.
Suppression of multiple anti‐apoptotic BCL2 family proteins recapitulates the effects of JAK2 inhibitors in JAK2V617F driven myeloproliferative neoplasms
Authors: Takei Hisashi , Coelho‐Silva Juan Luiz , Tavares Leal Cristina , Queiroz Arantes Rocha Adriana , Mantello Bianco Thiago , Welner Robert S. , Mishima Yuta , Kobayashi Ikei S. , Mullally Ann , Lima Keli , Machado‐Neto João Agostinho , Kobayashi Susumu S. , Lobo de Figueiredo‐Pontes Lorena 
Published: November 2021

AbstractSeveral lines of research suggest that Bcl‐xL‐mediated anti‐apoptotic effects may contribute to the pathogenesis of myeloproliferative neoplasms driven by JAK2V617F and serve as therapeutic target. Here, we used a knock‐in JAK2V617F mouse model and confirmed that Bcl‐xL was overexpressed in erythroid progenitors. The myeloproliferative neoplasm (MPN)‐induced phenotype in the peripheral blood by conditional knock‐in of JAK2V617F was abrogated by conditional knockout of Bcl2l1, which presented anemia and thrombocytopenia independently of JAK2 mutation status. Mx1‐Cre Jak2V617W/VF/Bcl2l1f/f mice presented persistent splenomegaly as a result of extramedullary hematopoiesis and pro‐apoptotic stimuli in terminally differentiated erythroid progenitors. The pan‐BH3 mimetic inhibitor obatoclax showed superior cytotoxicity in JAK2V617F cell models, and reduced clonogenic capacity in ex vivo assay using Vav‐Cre Jak2V617F bone marrow cells. Both ruxolitinib and obatoclax significantly reduced spleen weights in a murine Jak2V617F MPN model but did not show additive effect. The tumor burden reduction was observed with either ruxolitinib or obatoclax in terminal differentiation stage neoplastic cells but not in myeloid‐erythroid precursors. Therefore, disrupting the BCL2 balance is not sufficient to treat MPN at the stem cell level, but it is certainly an additional option for controlling the critical myeloid expansion of the disease.
The effects of maternal smartphone use on mother–child interaction
Authors: Lederer Yael , Artzi Hallel , Borodkin Katy 
Published: November 2021

AbstractThis study assessed the effects of maternal smartphone use on mother–child interaction. Thirty‐three Israeli mothers and their 24‐ to 36‐month‐old toddlers (16 boys) from middle‐high socioeconomic status participated in three within‐subjects experimental conditions: maternal smartphone use, maternal magazine reading, and uninterrupted dyadic free‐play. The mothers produced fewer utterances, provided fewer responses to child bids, missed child bids more often, and exchanged fewer conversational turns with their children when engaged with a smartphone or printed magazines compared to uninterrupted free‐play. The quality of maternal responsiveness was also decreased. These findings suggest maternal smartphone use compromises mother–child interaction, which given smartphone ubiquity in daily life may have negative effects on child development in various domains, including language, cognition, and socioemotional regulation.
A Survey on Deep Learning for Skeleton‐Based Human Animation
Authors: Mourot Lucas , Hoyet Ludovic , Le Clerc François , Schnitzler François , Hellier Pierre 
Published: November 2021

AbstractHuman character animation is often critical in entertainment content production, including video games, virtual reality or fiction films. To this end, deep neural networks drive most recent advances through deep learning (DL) and deep reinforcement learning (DRL). In this article, we propose a comprehensive survey on the state‐of‐the‐art approaches based on either DL or DRL in skeleton‐based human character animation. First, we introduce motion data representations, most common human motion datasets and how basic deep models can be enhanced to foster learning of spatial and temporal patterns in motion data. Second, we cover state‐of‐the‐art approaches divided into three large families of applications in human animation pipelines: motion synthesis, character control and motion editing. Finally, we discuss the limitations of the current state‐of‐the‐art methods based on DL and/or DRL in skeletal human character animation and possible directions of future research to alleviate current limitations and meet animators' needs.