Evaluation of Calcarea carbonica derivative complex (M8) on milk parameters in the dairy cow
Authors: Brandão André , Polo Giovanna , Da Lozzo Eneida Janiscki , Buchi Dorly Freitas , De Olivera Carolina , Weber Saulo Henrique , Ollhoff Daniel 
Published: December 2021

Background Any dairy herd that continually has a somatic cell count (SCC) above 200,000 cells/ml has an indication of mammary gland inflammation (mastitis). Routine use of antibiotics to prevent mastitis is prohibited by organic farming regulations. This limitation has lead researchers to focus on cows natural defense mechanisms [1]. Calcarea carbonica derivative complex (M8) is a complex high diluted medication comprised of comprised of Calcarea carbonica 16x, Aconitum napellus 20x, Arsenicum album 18x, Asa foetida 20x, Conium maculatum 17x, Ipecacuanha 13x, Phosphorus 20x, Rhus toxicodendron 17x, Silicea 20x, Sulphur 24x, and Thuya occidentalis 19x. Dilution procedures have followed standard methodology described at the Brazilian Homeopathic Pharmacopoeia. This medication has enhanced immune system responses both in vitro and in vivo in a murine model [2].
Aims In the present study, we investigate the response of dairy cows after M8 treatment.
Methodology The study was performed as a randomized, observer double-blinded and placebo-controlled trial, with a stratified design, using lactation number and SCC as stratification factors. The study sample consisted of 42 lactating dairy cows (Holstein) in one high producing dairy herd with 52 cows in milk in southern Brazil, divided into two experimental groups (n=21). Exclusion criteria were cows with clinical mastitis or receiving any other medical treatment. Pre- and post-milking teat disinfection was practiced in the herd. All cows were clinically examined, with udder and milk samples being appraised according to Rosenberger (1990) [3]. During 3 months one group received daily M8 treatment, the other placebo. Oral administration of 5 ml/day/cow was performed using an automatic dosage dispenser. Monthly, milk production, SCC, fat and total protein content were carefully recorded for each animal by an official milk recording program. SCC were log transformed for analysis. ANOVA and Tukey test were used to compare the averages. The BartlettÃƒâ€šÃ‚Â´s test was used for homogeneity of variance evaluation.
Results There were no significant differences (P=0.435) among the groups in the initial evaluation (Values of SCC x103 : Placebo 67,37Ãƒâ€šÃ‚Â±80,48; Treatment 359,39Ãƒâ€šÃ‚Â±677,02). After 3 months, the M8 treated group showed a decrease (134,00Ãƒâ€šÃ‚Â±178,76 P= 0.047) in SCC when compared with control group (391,71Ãƒâ€šÃ‚Â±686,60). Fat and protein did not differ between groups and time analysed. Milk production decreased in the placebo group during time (Before: 34,97Ãƒâ€šÃ‚Â±6,69kg; After:28,69Ãƒâ€šÃ‚Â±4,33kg), whereas the treatment group did not change total amount (Before: 28,7Ãƒâ€šÃ‚Â±6,54kg; After: 26,39Ãƒâ€šÃ‚Â±5,73kg; P > 0.05).
Conclusions These results indicate that the M8 influenced positively SCC and suggest that it may be considered as a possible tool to promote bovine mastitis prophylaxis.
Keywords: Calcarea carbonica complex, mastitis, somatic cell count
[1] McDougall S, Parker KI, Heuer C, Compton CWR. A review of prevention and control of heifer mastitis via non-antibiotic strategies. Vet Microbiol. 2009; 154:177ÃƒÂ¢Ã¢â€šÂ¬Ã¢â‚¬Å“85.
[2] Oliveira CC, Abud APR, Oliveira SM, GuimarÃƒÆ’Ã‚Â£es FSF, Andrade LF, Di Bernardi RP, Coletto ELO, Kuczera D, Da Lozzo EJ, GonÃƒÆ’Ã‚Â§alves JP, Trindade ES, Buchi DF. Developments on drug discovery and on new therapeutics: highly diluted tinctures act as biological response modifiers. BMC Complementary and Alternative Medicine. 2011; 11(101): 2-11.
[3]Rosenberger, G. Die klinische Untersuchung des Rindes, 3. Neubearbeitete Auflage, Paul Parey Verlag, Berlin, 1990.