bluuebunny/crossref_metadata_2025_split huggingface dataset:
The elimination of anticipated written errors from L1 interference through flipped classrooms
Authors: Prapawadee Napacha 
Published: January 2021

Several studies have been conducted on written errors discovered in an EFL classroom, and the findings are similar, and that common errors have been discovered in Thai students’ written work, so these studies conclude that the main cause of errors is L1 interference. In addition, other related studies reveal that flipped classrooms can support students’ learning process. It is due to the fact that these days, EFL students still have problems with some grammatical aspects due to their mother tongue, and the class time provided is limited. Therefore, to ascertain that the flipped classroom can help eliminate the anticipated grammatical problems repeatedly in class; the study looks to develop online lessons on LMS to encourage the students to learn at their own pace and to engage students with class activities. The online lessons and class activities are currently being implemented, and 25 freshmen participate in this study. The questionnaire will be completed at the end of the semester to discover their attitudes towards the flipped classroom covering online lessons and class activities. Also, a pre-test and post-test on simple sentences are used to investigate the students’ learning outcomes via the flipped classroom. The findings from the pre- and post-tests confirm the results of the former studies that the students produce the anticipated errors such as articles, capitalization, punctuation, nouns, and subject-verb agreement. Also, synchronous and asynchronous learning can help eliminate those mentioned errors and the students have positive attitudes towards the flipped classroom.Keywords: written errors; L1 interference; flipped classroom
A Non-coding HES1 Variant Predisposes Children to Congenital Heart Disease in Chinese Population
Authors: Song Yangliu , Chen Weicheng , Huang Zitong , Tian Guixiang , Li Mengru , Zhao Zhengshan , Feng Zhiyu , Wu Feizhen , Qian Maoxiang , Ma Xiaojing , Sheng Wei , Huang Guoying 
Published: January 2021

Background: As a key component in the NOTCH signaling pathway, HES1 plays an important role in vertebrate heart development. Variants in the HES1 coding sequence are known to be associated with congenital heart disease (CHD). However, little is known about HES1 non-coding sequence variants and their association with the risk of developing CHD.Method and Results: We initially analyzed the non-coding sequence of the HES1 gene in 12 unrelated CHD families by direct sequencing and identified a previously unreported promoter region variant (NM_005524.4: c.−1279−1278 insAC, rs148941464) in the HES1 gene in four CHD families. The homozygous variant in patients was inherited from carrier parents with normal phenotypes, indicating a likely recessive genetic model. Given that the HES1 gene is predicted to be likely to exhibit haploinsufficiency (%HI: 11.44), we hypothesized that the HES1 homozygous variant is a genetic risk factor underlying CHD. We then carried out sequencing of this HES1 variant in 629 sporadic non-syndromic CHD cases and 696 healthy controls and performed association analysis. Interestingly, we observed a significant association of the homozygous HES1 promoter variant with CHD (18.92% of cases vs. 9.91% of controls; OR: 2.291, 95% CI: 1.637-3.207, p = 9.72 × 10−7). No significant association with CHD was observed for the HES1 promoter heterozygous variant (p &gt; 0.05). However, association analysis tests of the HES1 homozygous variant with each subtype of CHD revealed that this homozygous variant was strongly associated with transposition of the great arteries (TGA) (OR: 3.726, 95% CI: 1.745-7.956, p = 0.0003). Moreover, the prevalence of HES1 homozygous variants in CHD patients with TGA (27.66%) was significantly higher than that in patients with other CHD subtypes or controls. Similar results were observed in a replication group of TGA (n = 64). Functional studies demonstrated that the homozygous variant in the HES1 promoter can disrupt its ability to bind RXRA, an inhibitory transcription factor, which results in abnormally high expression of the HES1 gene, indicating that this variant harbors gain-of-function effects.Conclusions: Our findings reveal that the non-coding homozygous variant in the HES1 promoter has a gain-of-function effect and is associated with an increased risk of CHD development, especially the severe TGA subtype.
Physicochemical Impacts of Dust Storms on Aerosol and Glacier Meltwater on the Northern Margin of the Taklimakan Desert
Authors: Zheng Cunying , Li Zhongqin , Zhou Ping , Zhang Xin , Zhou Xi , Ma Shan 
Published: January 2021

To analyze the impacts of dust storms on the atmospheric and meltwater environment, intensive and simultaneous sampling for aerosols and meltwater on the terminal moraine (41°73′ N, 79°88′ E, 3,789 m a.s.l.) of Qingbingtan Glacier No. 72 (Glacier No. 72) on the northern margin of the Taklamakan Desert were conducted during the summer of 2008. Aerosol and meltwater chemistry (including the species of Na+, K+, Mg2+, Ca2+, NH4+, Cl−, SO42−, and NO3−) was analyzed by ion chromatography. The results indicated that the major anions and cations in the samples were SO42− and Ca2+, respectively. In the dust event, the average value of aerosol ions was 566.91 neq·m−3, which was nearly five times that under nondust conditions (115.58 neq·m−3). In addition, in the meltwater samples, the sum of ion concentrations ranged from 31.26 to 180.98 mg·L−1, with an average of 76.40 mg·L−1. When a dust storm occurred, the ion concentrations in these two media increased significantly and simultaneously, but with different trends due to the different influencing factors. That is, the atmospheric environment was significantly affected by the dust storm. According to the correlation analysis and principal component analysis, the water-soluble ions, such as SO42−, Cl−, Na+, K+, Mg2+, and Ca2+, originated from natural sources, while NH4+ and NO3− originated from anthropogenic sources. The results of the air mass trajectory suggested that the regional dust storms caused by the air masses originating from Eastern Europe and Siberia had a significant influence on the glacial environment.
10-year Characteristics of Moisture Source Regions and Their Potential Effect on Seasonal Isotopic Signatures of δ18O in Tropical Trees of Southern Ecuador
Authors: Landshuter Nadja , Mölg Thomas , Grießinger Jussi , Bräuning Achim , Peters Thorsten 
Published: January 2021

Ratios of stable oxygen isotopes in tree rings (δ18O) are a valuable proxy for reconstructing past climates. Such reconstructions allow us to gain better knowledge of climate dynamics under different (eg warmer) environmental conditions, which also forms the basis for effective risk management. The latter aspect is particularly relevant for our study site on the western flanks of the Andes in Southern Ecuador, since the region is frequently affected by droughts and heavy precipitation events during the rainy season (January to April), leading to enormous social and economic losses. In particular, we focus on precipitation amounts and moisture source regions as they are known to influence the δ18O signature of tree rings. Moisture source regions are based on 240 h backward trajectories that were calculated with the trajectory model LAGRANTO for the rainy seasons 2008 to 2017. A moisture source diagnostic was applied to the air parcel pathways. The resulting moisture source regions were analyzed by calculating composites based on precipitation amounts, season, and calendar year. The precipitation amounts were derived from data of a local Automatic Weather Station (AWS). The analysis confirms that our study site receives its moisture both, from the Atlantic and the Pacific Oceans. Heavy precipitation events are linked to higher moisture contributions from the Pacific, and local SST anomalies along the coast of Ecuador are of higher importance than those off the coast toward the central Pacific. Moreover, we identified increasing moisture contributions from the Pacific over the course of the rainy season. This change and also rain amount effects are detectable in preliminary data of δ18O variations in tree rings of Bursera graveolens. These signatures can be a starting point for investigating atmospheric and hydroclimatic processes, which trigger δ18O variations in tree rings, more extensively in future studies.
Generating Pennycress (Thlaspi arvense) Seed Triacylglycerols and Acetyl-Triacylglycerols Containing Medium-Chain Fatty Acids
Authors: Esfahanian Maliheh , Nazarenus Tara J. , Freund Meghan M. , McIntosh Gary , Phippen Winthrop B. , Phippen Mary E. , Durrett Timothy P. , Cahoon Edgar B. , Sedbrook John C. 
Published: January 2021

Thlaspi arvense L. (pennycress) is a cold-tolerant Brassicaceae that produces large amounts of seeds rich in triacylglycerols and protein, making it an attractive target for domestication into an offseason oilseed cash cover crop. Pennycress is easily genetically transformed, enabling synthetic biology approaches to tailor oil properties for specific biofuel and industrial applications. To test the feasibility in pennycress of producing TAGs and acetyl-TAGs rich in medium-chain fatty acids (MCFAs; C6–C14) for industrial, biojet fuel and improved biodiesel applications, we generated transgenic lines with seed-specific expression of unique acyltransferase (LPAT and diacylglycerol acyltransferase) genes and thioesterase (FatB) genes isolated from Cuphea viscosissima, Cuphea avigera var. pulcherrima, Cuphea hookeriana, Coco nucifera, and Umbellularia californica. Wild-type pennycress seed TAGs accumulate no fatty acids shorter than 16C and less than 5 mol percent C16 as palmitic acid (16:0). Co-expressing UcFatB and CnLPAT produced up to 17 mol% accumulation of lauric acid (12:0) in seed TAGs, whereas CvFatB1 CvLPAT2 CpDGAT1 combinatorial expression produced up to 27 mol% medium chain FAs Medium Chain Fatty Acids mostly in the form of capric acid (10:0). CpFatB2 ChFatB2 combinatorial expression predominantly produced, in equal parts, up to 28 mol% myristic acid (14:0) and palmitic acid. Genetically crossing the combinatorial constructs into a fatty acid elongation1 (fae1) mutant that produced no 22:1 erucic acid, and with an Euonymus alatus diacylglycerol acetyltransferase (EaDAcT)-expressing line that produced 60 mol% acetyl-TAGs, had no or relatively minor effects on MCFAs accumulation, suggesting fluxes to MCFAs were largely unaltered. Seed germination assays revealed no or minor delays in seed germination for most lines, the exception being CpFatB2 ChFatB2-expressing lines, which had substantially slower seed germination rates. Taken together, these data show that pennycress can be engineered to produce seeds accumulating modest amounts of MCFAs of varying carbon-chain length in TAGs and acetyl-TAGs, with rates of seed germination being delayed in only some cases. We hypothesize that increasing MCFAs further may require functional reductions to endogenous transferases and/or other FA elongases.
Elevated Circulating IL-10 Producing Breg, but Not Regulatory B Cell Levels, Restrain Antibody-Mediated Rejection After Kidney Transplantation
Authors: Luo Yongsheng , Luo Feifei , Zhang Kuanxin , Wang Shilei , Zhang Haojie , Yang Xianlei , Shang Wenjun , Wang Junxiang , Wang Zhigang , Pang Xinlu , Feng Yonghua , Liu Lei , Xie Hongchang , Feng Guiwen , Li Jinfeng 
Published: January 2021

BackgroundAntibody-mediated rejection (AMR) occupies a major position for chronic rejection after kidney transplantation. Regulatory B cell (Breg) has been reported to have an inhibitory immune function, which contributes to the resistance for AMR.MethodsA nested case–control study for nine healthy donors, 25 stable (ST) patients, and 18 AMR patients was performed to determine the type of Breg in maintaining immune tolerance and preventing AMR.ResultsCompared to the ST group, circulating interleukin (IL)-10+ Bregs, but not Bregs, significantly decreased. The receiver operating characteristic (ROC) curve analysis revealed that rather than the circulating Bregs, decreased circulating IL-10+ Breg levels were positively associated with AMR. However, kidney B cell and IL-10 infiltration was significantly increased in the AMR group with high expression of C-X-C motif chemokine 13 (CXCL13). In addition, circulating IL-10+ Bregs, rather than Bregs, remained higher than those at pre-operation, during the 90-day post-operation in immune homeostasis.ConclusionThe circulating IL-10+ Breg levels are more appropriate measures for assessing the resistance of AMR after kidney transplantation.
MAC Project—Monitoring Anticoagulant Therapy Observational Study: Rationale and Protocol
Authors: Camporese Giuseppe , Bernardi Enrico , Bortoluzzi Cristiano , Noventa Franco , Hong Ngoc Vo , Callegari Elena , Villalta Sabina , Tonello Chiara , Nardin Michela , Campello Elena , Spiezia Luca , Simioni Paolo 
Published: January 2021

Real-life studies complement data from registrative trials. Because of the delayed registration of direct oral anticoagulants in Italy, scarce real-life data on such treatments is available for the Italian population. The aim of the MAC project is to collect real-life clinical information in unselected patients given oral anticoagulants for venous thromboembolism, during a 5-year follow-up period. This is a prospective-cohort, multi-center, observational study performed in four Italian centers. The estimated samples size is 4,000 patients. The efficacy outcomes are: incidence of symptomatic recurrent venous thromboembolism and of post-thrombotic syndrome. The safety outcomes are: incidence of major bleeding, clinically relevant non-major bleeding, minor bleeding, serious adverse events, and mortality. The MAC project has the potential to improve our understanding of the epidemiology and of the therapeutic strategies adopted in Italian patients with venous thromboembolism.Clinical Trial Registration: WWW.ClinicalTrials.Gov, identifier: NCT0432939.
Artificial Intelligence for the Future Radiology Diagnostic Service
Authors: Mun Seong K. , Wong Kenneth H. , Lo Shih-Chung B. , Li Yanni , Bayarsaikhan Shijir 
Published: January 2021

Radiology historically has been a leader of digital transformation in healthcare. The introduction of digital imaging systems, picture archiving and communication systems (PACS), and teleradiology transformed radiology services over the past 30 years. Radiology is again at the crossroad for the next generation of transformation, possibly evolving as a one-stop integrated diagnostic service. Artificial intelligence and machine learning promise to offer radiology new powerful new digital tools to facilitate the next transformation. The radiology community has been developing computer-aided diagnosis (CAD) tools based on machine learning (ML) over the past 20 years. Among various AI techniques, deep-learning convolutional neural networks (CNN) and its variants have been widely used in medical image pattern recognition. Since the 1990s, many CAD tools and products have been developed. However, clinical adoption has been slow due to a lack of substantial clinical advantages, difficulties integrating into existing workflow, and uncertain business models. This paper proposes three pathways for AI's role in radiology beyond current CNN based capabilities 1) improve the performance of CAD, 2) improve the productivity of radiology service by AI-assisted workflow, and 3) develop radiomics that integrate the data from radiology, pathology, and genomics to facilitate the emergence of a new integrated diagnostic service.
Endovascular Treatment of Ruptured Middle Cerebral Artery Aneurysms With a Low-Profile Visualized Intraluminal Support Device
Authors: Xue Gaici , Zhou Yu , Liu Peng , Zuo Qiao , Yang Pengfei , Fang Yibin , Li Qiang , Zhao Rui , Xu Yi , Hong Bo , Huang Qinghai , Liu Jianmin 
Published: January 2021

Objective: Stenting in ruptured middle cerebral artery (MCA) aneurysms was reported with a high perioperative complication rate. However, the treatment devices and physician's experience have continued to evolve. We performed this retrospective study to evaluate the safety and efficacy of LVIS stent-assisted coiling for ruptured MCA aneurysms.Methods: Patients with acutely ruptured MCA aneurysms treated between November 2014 and October 2019 were retrospectively reviewed. Clinical and angiographic data of those treated with LVIS stents were collected from a prospectively maintained database.Results: A total of 40 patients with 40 ruptured MCA aneurysms were enrolled, which comprised 26.3% (40/152) of all the ruptured MCA aneurysms at the same time. All stents were successfully deployed except for one (2.5%), which had a poor stent opening. Ischemic procedure-related complications were encountered in three patients (7.5%). One patient died of complications related to high-grade SAH on admission. Follow-up (mean 15.9 months) angiography was performed for 36 patients, which showed 33 (91.7%) aneurysms were completely occluded, 1 (2.8%) was improved, 1 (2.8%) was stable, and 1 (2.8%) was recanalized. Clinical follow-up (mean 29.6 months) was available for all survived patients, which showed 38 (95.0%) patients had favorable neurologic outcomes (mRS score 0–2), and 2 (5.0%) patients had poor neurologic outcomes.Conclusion: The use of LVIS stents is feasible, safe, and effective with glycoprotein IIb/IIIa inhibitor for the treatment of ruptured MCA aneurysms in the acute setting. Prospective, multicenter studies with larger sample sizes are still required to further evaluate the safety and long-term efficacy.
Enhanced Hemodynamic and Clinical Response to αCGRP in Migraine Patients—A TCD Study
Authors: Visočnik Darja , Zaletel Marjan , Žvan Bojana , Zupan Matija 
Published: January 2021

Introduction: Sensitisation of the nervous system in a patient with migraine is supposed to be associated with calcitonin gene-related peptide (CGRP) activity. Therefore, the vascular response to human αCGRP (hαCGRP) could be a surrogate marker for the sensitization. We hypothesize that vascular response to hαCGRP is augmented in a patient with migraine.Methods: Twenty healthy subjects and 20 patients with migraine participated in our study. TCD was used to monitor mean arterial velocity in the middle cerebral artery (vm MCA). Simultaneously, end-tidal CO2 (Et-CO2), mean arterial pressure (MAP), and heart rate (HR) were measured. The reconstruction of the signals was made for basal conditions, during and after CGRP infusion which were compared using statistics.Results: In both groups, we found significant decrease between measurement points of vm MCA and Et-CO2 during and after hαCGRP infusion. MAP did not show significant trends during the infusion, but it was significantly increased after the infusion in migraine patients only. Responses to hαCGRP, defined as differences between two measurement points, were significantly higher for vm MCA and Et-CO2 in patients with migraine. A significant difference between groups was found in MAP response. Significant relationships were found between migraine and vm MCA, Et-CO2, and MAP.Conclusion: In patients with migraine, vm MCA responses to hαCGRP are significantly higher and are associated with CGRP-induced headache which indicates that patients with migraine are more prone to sensitization.
Viability of Preictal High-Frequency Oscillation Rates as a Biomarker for Seizure Prediction
Authors: Scott Jared M. , Gliske Stephen V. , Kuhlmann Levin , Stacey William C. 
Published: January 2021

Motivation: There is an ongoing search for definitive and reliable biomarkers to forecast or predict imminent seizure onset, but to date most research has been limited to EEG with sampling rates &lt;1,000 Hz. High-frequency oscillations (HFOs) have gained acceptance as an indicator of epileptic tissue, but few have investigated the temporal properties of HFOs or their potential role as a predictor in seizure prediction. Here we evaluate time-varying trends in preictal HFO rates as a potential biomarker of seizure prediction.Methods: HFOs were identified for all interictal and preictal periods with a validated automated detector in 27 patients who underwent intracranial EEG monitoring. We used LASSO logistic regression with several features of the HFO rate to distinguish preictal from interictal periods in each individual. We then tested these models with held-out data and evaluated their performance with the area-under-the-curve (AUC) of their receiver-operating curve (ROC). Finally, we assessed the significance of these results using non-parametric statistical tests.Results: There was variability in the ability of HFOs to discern preictal from interictal states across our cohort. We identified a subset of 10 patients in whom the presence of the preictal state could be successfully predicted better than chance. For some of these individuals, average AUC in the held-out data reached higher than 0.80, which suggests that HFO rates can significantly differentiate preictal and interictal periods for certain patients.Significance: These findings show that temporal trends in HFO rate can predict the preictal state better than random chance in some individuals. Such promising results indicate that future prediction efforts would benefit from the inclusion of high-frequency information in their predictive models and technological architecture.
Survival Predictors of Heart Rate Variability After Myocardial Infarction With and Without Low Left Ventricular Ejection Fraction
Authors: Hayano Junichiro , Ueda Norihiro , Kisohara Masaya , Yuda Emi , Carney Robert M. , Blumenthal James A. 
Published: January 2021

BackgroundHeart rate variability (HRV) and heart rate (HR) dynamics are used to predict the survival probability of patients after acute myocardial infarction (AMI), but the association has been established in patients with mixed levels of left ventricular ejection fraction (LVEF).ObjectiveWe investigated whether the survival predictors of HRV and HR dynamics depend on LVEF after AMI.MethodsWe studied 687 post-AMI patients including 147 with LVEF ≤35% and 540 with LVEF &gt;35%, of which 23 (16%) and 22 (4%) died during the 25 month follow-up period, respectively. None had an implanted cardioverter-defibrillator. From baseline 24 h ECG, the standard deviation (SDNN), root mean square of successive difference (rMSSD), percentage of successive difference &gt;50 ms (pNN50) of normal-to-normal R-R interval, ultra-low (ULF), very-low (VLF), low (LF), and high (HF) frequency power, deceleration capacity (DC), short-term scaling exponent (α1), non-Gaussianity index (λ25s), and the amplitude of cyclic variation of HR (Acv) were calculated.ResultsThe predictors were categorized into three clusters; DC, SDNN, α1, ULF, VLF, LF, and Acv as Cluster 1, λ25s independently as Cluster 2, and rMSSD, pNN50, and HF as Cluster 3. In univariate analyses, mortality was best predicted by indices belonging to Cluster 1 regardless of LVEF. In multivariate analyses, however, mortality in patients with low LVEF was best predicted by the combinations of Cluster 1 predictors or Cluster 1 and 3 predictors, whereas in patients without low LVEF, it was best predicted by the combinations of Cluster 1 and 2 predictors.ConclusionThe mortality risk in post-AMI patients with low LVEF is predicted by indices reflecting decreased HRV or HR responsiveness and cardiac parasympathetic dysfunction, whereas in patients without low LVEF, the risk is predicted by a combination of indices that reflect decreased HRV or HR responsiveness and indicator that reflects abrupt large HR changes suggesting sympathetic involvement.