bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Expression of FLNA and CLU in PBMCs As A Novel Screening Marker for Hepatocellular Carcinoma.
Authors: Patarat Rathasapa , Riku Shoji , Kunadirek Pattapon , Chuaypen Natthaya , Tangkijvanich Pisit , Mutirangura Apiwat , Puttipanyalears Charoenchai 
Published: February 2021

Abstract
Early detection improves survival and increases curative probability in hepatocellular carcinoma (HCC). Peripheral blood mononuclear cells (PBMCs) can provide an inexpensive, less-invasive and highly accurate method. The objective of this study is to find the potential marker for HCC screening, utilizing gene expression of the PBMCs. Data from the NCBI GEO database of gene expression in HCC patients and healthy donor's PBMCs was collected. As a result, GSE 49515 and GSE 58208 were found. Using both, a statistical significance test was conducted in each gene expression of each data set which resulted in 187 genes. We randomized three selected genes (FLNA, CAP1, and CLU) from the significant p-value group (p-values < 0.001). Then, a total of 76 healthy donors and 153 HCC cases were collected. Quantitative RT-PCR (qRT-PCR) was performed in cDNA from all blood samplesFrom the qRT-PCR, The Cycle threshold (Ct) value of FLNA, CLU, CAP1 of HCC group (28.47±4.43, 28.01±3.75, 29.64±3.90) were lower than healthy group (34.23±3.54, 32.90±4.15, 32.18±5.02) (p-values < 0.0001). The accuracy, sensitivity and specificity of these genes as a screening tool were: FLNA (80.8%, 88.0%, 65.8%), CLU (63.4%, 93.3%, 31.3%), CAP1 (67.2%, 83.3%, 39.1%). The tests were performed in two and three gene combinations. Results demonstrated high accuracy of 86.2%, sensitivity of 85% and specificity of 88.4% in the FLNA and CLU combination. We concluded that FLNA and CLU combination have high potential for being HCC novel markers. Combined with current tumor markers, further research of the gene’s expression might help identify more potential markers and improve diagnosis methods.
Improved Hole Wall Roughness and Corrosion Resistance of U-Shaped Hole Prepared by Casting
Authors: Qu Yingdong , Cao Yang , Li Guanglong , Chen Ruirun , Li Rongde , Nie Sainan , Tian Chang 
Published: February 2021

Abstract
The corrosion resistance of the cooling water channel in the water-cooled mold has an important impact on the application of the mold. In this paper, the influence of experimental temperature on roughness and corrosion resistance of U-shaped cooling channels prepared by casting is analyzed in detail. The results show that the experimental temperature increased from 998K to 1048K, the hole wall roughness of the U-shaped hole decreased from 83.264 to 76.287. However, the hole wall roughness increased with a further increase in temperature. Because the low experimental temperature will promote the formation of micro bulges and river ripples on the hole wall. However, with the temperature gradually increased, it will promote the aluminum matrix to react with the carbon fiber, which will increase the roughness. The corrosion performance analysis results show that a reasonable experimental temperature is beneficial to reduce the roughness of the hole wall and improve the corrosion resistance of the cooling channel. When the roughness is large, the ravines on the rough surface are easy to be the starting point of corrosion. At the same time, it is easy to cause the aggregation of Si elements to the hole wall at high experimental temperature, which will also cause corrosion. As a result, reasonable experimental temperature plays an important role in improving the corrosion resistance of the U-shaped cooling channel.
RhoBTB3 Regulates Proliferation and Invasion of Breast Cancer Cells via Col1A1
Authors: kim kyungho , Kim Youn-Jae 
Published: February 2021

Abstract
Background: Breast cancer is the leading cause of cancer-related death in women worldwide, despite medical and technological advancements. The RhoBTB family consists of three isoforms: RhoBTB1, RhoBTB2, and RhoBTB3. RhoBTB1 and RhoBTB2, have been proposed as tumor suppressors in breast cancer. However, the roles of RhoBTB3 proteins are unknown in breast cancer. Methods: Bioinformatics analysis, including Oncomine, cBioportal, was used to evaluate the potential functions and prognostic values of RhoBTB3 and Col1A1 in breast cancer. qRT-PCR analysis and immunoblotting assay were performed to investigate relevant expression. Functional experiments including proliferation assay, invasion assay, and flow cytometry assay were conducted to determine the role of RhoBTB3 and Col1A1 in breast cancer cells. Results: RhoBTB3 mRNA levels were significantly up-regulated in breast cancer tissues as compared to in adjacent normal tissues. Moreover, RhoBTB3 expression was found to be associated with Col1A1 expression. Decreasing RhoBTB3 expression may lead to decreases in the proliferative and invasive properties of breast cancer cells. Further, Col1A1 knockdown in breast cancer cells limited the proliferative and invasive ability of cancer cells. Conclusion: Knockdown of RhoBTB3 may exert inhibit the proliferation, migration, and metastasis of breast cancer cells by repressing the expression of COL1A1, providing a novel therapeutic strategy for treating breast cancer. Keywords: RhoBTB3, Col1A1, human breast cancer
Design of Ag Injection in Bi2S3 (AgBiS2) Thin Films for Photoelectrochemical Cell and Solar Cell Applications
Authors: T Daniel , V Balasubramanian , G Sivakumar , Mohanraj Kannusamy 
Published: February 2021

Abstract
This study reports the opto-structural, morphological, topological and electrical properties of thermally evaporated AgxBi2-xS3-y thin film prepared for various x and y values (x= y= 0, 0.25, 0.50, 0.75 and 1). The films have cubic structured AgBiS2 along with orthorhombic structured Bi2S3 as confirmed from X-ray diffraction (XRD) analysis. The films showed higher optical absorption coefficient (105cm-1) in the visible region and band gap values are found to be decreased from 2.08 eV to 1.35 eV for AgxBi2-xS3-y (x= y = 0 to 1) films. Scanning electron microscope (SEM) images showed the uniform distribution of spherical particles. Carrier concentration of the films are better than x= y= 0 as observed from Hall effect and Mott- Schottky plots. The FTO/ AgxBi2-xS3-y (x= y = 1) photoelectrochemical cell yields the photoconversion efficiency (PCE) of 7.03 %. The device FTO/ AgxBi2-xS3-y (x= y = 1) CdS/Ag solar cell has exhibited PCE of 3.26%.
Verbal Labels Facilitate Tactile Texture Discrimination in a Perceptual Learning Task
Authors: Arun Ishita , Lazar Leslee 
Published: February 2021

Abstract
The influence of language on perceptual processes, referred to as the Whorfian hypothesis, has been a contentious issue. Cross-linguistic research and lab-based experiments have shown that verbal labels can facilitate perceptual and discriminatory processes, mostly in visual and auditory modalities. Here, we investigated whether verbal labels improve performance in a tactile texture discrimination task using natural textures. We also explored whether the grammatical category of these verbal labels plays a role in discrimination ability. In our experiments, we asked the participants to discriminate between pairs of textures presented to the fingertip after a five-day training phase. During the training phase, the tactile textures and English pseudowords were co-presented consistently in the congruent (experimental) condition and inconsistently in the incongruent (control) condition, allowing them to form implicit associations only in the former condition. The pseudoword verbal labels belonged to two grammatical categories, verb-like and noun-like. We found an improvement in the texture discrimination ability only for the congruent condition, irrespective of the grammatical category.
The Preventive and Treatment of the Neuro-Inflammasome in Sorokdo National Hospital
Authors: Lee Jong-hoon , Lee Chul Joong , Park Jungwuk , Lee So Jeong , Choi Su-Hee , Oh Sang-Suk 
Published: February 2021

Abstract
Aim/Background: This study investigated patients with Alzheimer's disease (AD) treated with 4,4’-diaminodiphenyl sulfone (DDS) as a neuro-inflammasome competitor. Method: The Seoul study analyzed AD, and anti-AD drugs (AADs) in the Sorokdo National Hospital's EDI database archived from January 2005 to June 2020 through the ICD-9 and -10 codes. Result: DDS acts as a neuro-inflammasome competitor; this effect can be inferred by comparing the prevalence of AD in patients who have been prescribed DDS and those who have not. Conclusion: This study suggests the use of neuro-inflammasome therapy as a preventive and therapeutic method for AD.
Profiles for Identifying Problematic Dietary Habits in a Sample of Recreational Spanish Cyclists and Triathletes
Authors: Muros José J. , Knox Emily , Hinojosa-Nogueira Daniel , Rufián-Henares José Á. , Zabala Mikel 
Published: February 2021

Abstract
Background
There is a lack of sufficient information on the dietary intake and nutritional supplementation of recreational endurance athletes throughout the year. The present observational study sought to assess the dietary intake and nutritional supplementation habits of recreational cyclists and triathletes from Spain.
Methods
4,037 cyclists and triathletes completed self-report measures. Nutritional profiles were developed and differences were examined according to sporting discipline and gender. Differences between groups were compared using the Mann-Whitney U or chi-squared test. Next, micro- and macro-nutrients were grouped according to whether or not guideline intake amounts were met. The clustering of dietary habits was then examined via K-means cluster analysis.
Results
Triathletes took more supplements than cyclists (X2 = 36.489; p value = .000) and females took more supplements than males (X2 = 5.920; p value = .017). Females and triathletes reported greater protein and CHO consumption than males and cyclists, respectively. Triathletes also reported a higher consumption of total fat, MUFA, PUFA, EPA, DHA and fibre. Females and triathletes tended to consume more vitamins and minerals than males and cyclists, respectively. Two main dietary habit clusters emerged which may be used to inform nutritional interventions targeting recreational athletes not meeting nutritional requirements.
Conclusions
There is an imbalance in the main nutrients making up the diet of recreational Spanish athletes, characterised by insufficient CHO and excessive protein.
Naturalistic Exploratory Study of the Associations of Substance Use on ADHD Outcomes and Function
Authors: MacDonald Benjamin , Sadek Joseph 
Published: February 2021

Abstract
Background: Although Attention Deficit Hyperactivity Disorder (ADHD) is associated with an increased risk of substance use disorder (SUD), existing literature on how SUD interacts with ADHD outcomes is limited. This study investigates whether SUD among individuals with ADHD is associated with worse ADHD outcomes and prognosis, and the association between overall functioning and SUD. In addition, we seek to understand whether heavy cannabis use is a better predictor of poorer outcomes compared to SUD status alone. Method: We conducted a retrospective analysis on 50 ADHD patient charts, which were allocated based on SUD status. Subgroup analysis was performed on the total sample population, with allocation based on heavy cannabis use.Mann-Whitney and Chi-Square tests were used for both the primary and subgroup analyses. Results: SUD status highly correlated with more ADHD-related cognitive impairments and poorer functional outcomes at the time of diagnosis. ADHD patients with comorbid ADHD-SUD scored significantly lower (p=<0.0001) on objective cognitive testing (the continuous performance test). The correlation with poorer ADHD outcomes was more pronounced when groups were allocated based on heavy cannabis use status, where in addition to significantly lower objective cognitive testing scores (p=0.0011), heavy cannabis use was associated with more severe fine motor hyperactivity and self-reported hyperactivity/impulsivity scores (p=0.0088 and 0.0172, respectively). Conclusion: Future research is needed to determine how substance abuse can be a barrier to improved ADHD outcomes, and the effect cannabis and other substances have on cognitive function and pharmacotherapy of ADHD.
The Effect of the Excretion of Calcium, Magnesium, and Phosphate on the Serum Levels of These Substances in Newborns Who Therapeutic Hypothermia for Hypoxic Ischemic Encephalopathy
Authors: Baştuğ Osman , İnan Bahadır , Özdemir Ahmet , Çelik Binnaz , Baştuğ Funda , Karakükcü Çiğdem 
Published: February 2021

Abstract
Background: Hypocalcemia, hypomagnesemia, and hyperphosphatemia are common electrolyte disturbances in perinatal asphyxia(PA). Different reasons have been proposed for these electrolyte disturbances. This study investigated the effect of the urinary excretion of calcium(Ca), magnesium(Mg), and phosphorus(P) on the serum levels of these substances in babies who were treated using therapeutic hypothermia for hypoxic ischemic encephalopathy(HİE) caused by PA. This study sheds light on the pathophysiology that may cause changes in the serum values of these electrolytes.Method: This study included 21 healthy newborns(control group) and 38 patients(HİE group) who had undergone therapeutic hypothermia due to HİE. Only infants with a gestational age of 36 weeks and above and a birth weight of 2000 g and above were evaluated. The urine and serum Ca, Mg, P, and creatinine levels of all infants were evaluated at 24, 48, and 72 hours.Results: The lower serum Ca value and the higher serum P value of the HİE group were found to be statistically significant compared to the control group. There was no significant difference in serum Mg values between the groups. However, hypomagnesemia was detected in five patients from the HİE group. The urine excretions of these substances, which were checked at different times, were found to be significantly higher in the HİE group compared to the control group.Conclusion: This study determined that the urinary excretion of Ca, Mg, and P has an effect on the serum Ca, Mg, and P levels of infants with HİE.
Effect of the Glucagon-like Peptide-1 Receptor (GLP-1R) Agonists on Autonomic Function in Subjects With Diabetes: a Systematic Review and Meta-analysis.
Authors: Greco Carla , Santi Daniele , Brigante Giulia , Pacchioni Chiara , Simoni Manuela 
Published: February 2021

Abstract
Background. In addition to the metabolic effects in diabetes, glucagon-like peptide 1 receptor (GLP-1R) agonists lead to a small but substantial increase in heart rate (HR). However, the GLP-1R actions on the autonomic nervous system (ANS) in people with diabetes remain still debated. Therefore, this meta-analysis evaluates the effect of GLP-1R agonist chronic treatment on measures of ANS function in people with diabetes. Methods. According to the Cochrane Collaboration and PRISMA statement, we conducted a meta-analysis considering clinical trials in which the autonomic function was evaluated in people with diabetes chronically treated with GLP-1R agonists. The outcomes were the change of ANS function measured by heart rate variability (HRV) and cardiac autonomic reflex tests (CARTs). Results. In the studies enrolled, HR significantly increased after treatment (p<0.001), whereas low frequency/high frequency ratio did not differ (p=0.410); no changes in other measures of HRV were detected. Considering CARTs, only the 30:15 value derived from lying-to-standing test was significantly lower after treatment (p=0.002), but only two studies reported this measurement. No differences in other CARTs outcome were observed. Conclusion. The present meta-analysis confirms the HR increase but seems to exclude an alteration of the sympatho-vagal balance due to chronic treatment with GLP-1R agonists in diabetes, considering the available measures of ANS function.
The engineered tetra-valent antibody, ASP4021, fully activates the Tie2 receptor with comparable potency to its natural ligand, angiopoietin-1
Authors: Koya Yukari , Nara Hiromi , Yagi Shigenori , Ueno Chihoko , Kamohara Masazumi 
Published: February 2021

Abstract
Activation of the tyrosine kinase with Ig and epidermal growth factor homology domain 2 (Tie2) receptor by angiopoietin-1 (Ang1) is critical for vascular stabilization: it promotes survival signal transduction via auto-phosphorylation and reduces vascular permeability by strengthening tight junctions between endothelial cells. Thus, Tie2/Ang1 signaling is a promising therapeutic target for vascular diseases. However, in vivo use of existing Tie2 signaling modulators, such as recombinant Ang1, is restricted by limitations in manufacturability and stability. Here, we present a novel engineered tetra-valent agonistic antibody, ASP4021, which can specifically and fully activate the Tie2 receptor in an equivalent manner to Ang1. ASP4021 induced Tie2 self-phosphorylation and inhibited apoptosis in a human primary endothelial cell line. Additionally, single administration of ASP4021 significantly suppressed mustard-oil-induced vascular permeability in rats. ASP4021 may thus be a potential therapeutic candidate for diseases associated with vascular weakness such as diabetic retinopathy, diabetic macular edema and critical limb ischemia.
Inter-observer and Intra-observer Variability in Gross Tumor Volume Delineation of Primary Esophageal Carcinomas Based on Different Combinations of Diagnostic Multimodal Images.
Authors: Li Fengxiang , Li Yankang , Wang Xue , Li Jianbin , Zhang Yingjie , Liu Xijun , Liu Shanshan , Wang Wei , Wang Jinzhi , Guo Yanluan , Xu Min 
Published: February 2021

Abstract
Background and purpose This study aimed to investigate inter-/ intra-observer delineation variability in GTVs of primary esophageal carcinomas (ECs) based on planning CT with reference to different combinations of diagnostic multimodal images from endoscopy/EUS, esophagography and FDG-PET/CT. Materials and methods Fifty-one patients with pathologically proven thoracic EC who underwent diagnostic multimodal images before concurrent chemoradiotherapy were enrolled. Five radiotherapists independently delineated the GTVs based on planning CT only (GTVC), CT combined with endoscopy/EUS (GTVCE), CT combined with endoscopy/EUS and esophagography (X-ray) (GTVCEX), and CT combined with endoscopy/EUS, esophagography, and FDG-PET/CT (GTVCEXP). The intra-/inter-observer variability in the volume, longitudinal length, generalized CI (CIgen), and position of the GTVs were assessed. Results The intra-/inter-observer variability in the volume and longitudinal length of the GTVs showed no significant differences (p>0.05). The mean intra-observer CIgen values for all observers was 0.73±0.15. The mean inter-observer CIgen values for the four multimodal image combinations was 0.67±0.11. The inter-observer CIgen for the four combined images was the largest, showing significant differences with those for the other three combinations. The intra-observer CIgen among different observers and inter-observer CIgen among different combinations of multimodal images showed significant differences (p<0.001). The intra-observer CIgen for the senior radiotherapists was larger than that for the junior radiotherapists (p<0.001) . Conclusion For radiotherapists with advanced medical imaging training and clinical experience, using diagnostic multimodal images from endoscopy/EUS, esophagography, and FDG-PET/CT could reduce the intra-/inter-observer variability and increase the accuracy of target delineation in primary esophageal carcinomas.
Performance Analysis of Channel Estimation in 5G millimeter-Wave-MIMO Heterogeneous Systems
Authors: CHOPRA SANJEEV , Kakkar Ajay 
Published: February 2021

Abstract
Millimeter Wave (mm-Wave) - massive Multiple-Input Multiple-Output (MIMO) technology has been a subject of today’s growing interest in both industry and academia for future wireless standards and has significant potential to provide considerable gains in data rates, link reliability and Energy Efficiency (EE).Sparse recovery has great capability in Channel Estimation (CE) for mm-Wave - Massive-MIMO (Ma-MIMO) heterogeneous wireless networks and in this context; the existing better candidate “Orthogonal Matching Pursuit” (OMP) algorithm is modified for CE in such networks. This paper will provide an opportunity in setting up of such a network, and the practical observation of the effect of a change in threshold, sparsity and noise levels as well as quantity of Radio Frequency (RF) chain systems. The performance analysis of CE accuracy in terms of Normalized Mean Square Error (NMSE)verses a given Signal-to-Noise (SNR) range in dB is calculated using this modified algorithm, much better than the existing OMP methods and compared against the ideal Genie case under a wide range of noise encountered. It has been observed that in a very high noise environment, NMSE of this noise resistant algorithm is approximate (100.5 to 10-3.5) in low SNR range (-10 to 30) dB and approximate (10-1.5 to10-5.5) in high SNR range (10 to 50) dB. It comes out to be approximate (10-1 to more than 10-5) in case of combined effect involvingthe reduction of quantity of RF chain systems to half and 10 times enhancement of threshold level in a very high noise environment and low SNR range.
Effect of Various PCBM Doping on the Interfacial Layer of Al/PCBM:ZnO/p-Si Photodiodes
Authors: Öztürk Teoman 
Published: February 2021

Abstract
The light can be detected by various devices such as photoconductors, photodetectors, phototransistors, solar cells and photodiodes, and the light can be employed energy harvesting as well as sensing applications. Various materials can be utilized to raising of the efficiency of light-based devices. In present study, metal-semiconductor (MS) devices with various [6,6]-Phenyl C61-butyric acid methyl ester (PCBM) doped zinc oxide (ZnO) interfacial layer were fabricated for photodiode applications. Commercially purchased PCBM and ZnO were mixed in order to obtain undoped, 3%, 5%, and 10% PCBM doped ZnO interfacial layer for Al/PCBM:ZnO/p-Si devices. The obtained solutions were layered on the p-type silicon (p-Si) substrades by spin coating method. The thermal evaporation technique was utilized to deposit Al metal electrodes both back and front sides. The morphological properties of the fabricated devices were characterized by AFM. The morphological images of the AFM revealed that PCBM doping affected the surface morphology as well as surface roughness of the ZnO layers. I-V measurements were carried out in the dark and under varying illumination conditions for electrical properties. The devices exhibited good rectifying properties at around 103 rates, both dark and various illumination conditions, according to I-V graphs. The junction parameters of the produced devices were determined by using thermionic emission, Norde, and Cheung models from the I-V characteristics of the devices. The devices have high ideality factors and these values generally fluctuate with varying PCBM doping amounts. The current transient measurements show that PCBM doping provide increasing in the light response capacity. According to the results, the devices can be employed as photodiode and photodetector applications for the further works in the industry.