bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Draconian Law: Examining the Navigation of Coalition Politics and Policy Reform by Health Provider Associations in Karnataka, India
Authors: Mishra Arima , Elias Maya Annie , Sriram Veena 
Published: January 2021

AbstractA comprehensive picture of provider coalitions in health policy making remains incomplete because of the lack of empirically driven insights from low- and middle-income countries. The authors examined the politics of provider coalitions in the health sector in Karnataka, India, by investigating policy processes between 2016 and 2018 for developing amendments to the Karnataka Private Medical Establishments Act. Through this case, they explore how provider associations function, coalesce, and compete and the implications of their actions on policy outcomes. They conducted in-depth interviews, document analysis, and nonparticipant observations of two conferences organized by associations. They found that provider associations played a major role in drafting the amendments and negotiating competing interests within and between doctors and hospital associations. Despite the fragmentation, the associations came together to reinterpret the intentions of the amendments as being against the interests of the profession, culminating in a statewide protest and strike. Despite this show of strength, provider associations only secured modest modifications. This case demonstrates the complex and unpredictable influence of provider associations in health policy processes in India. The authors' analysis highlights the importance of further empirical study on the influence of professional and trade associations across a range of health policy cases in low- and middle-income countries.
Resolving the heterogeneity of diaphragmatic mesenchyme: a novel mouse model of congenital diaphragmatic hernia
Authors: Cleal Louise , McHaffie Sophie L. , Lee Martin , Hastie Nick , Martínez-Estrada Ofelia M. , Chau You-Ying 
Published: January 2021

ABSTRACT
Congenital diaphragmatic hernia (CDH) is a relatively common developmental defect with considerable mortality and morbidity. Formation of the diaphragm is a complex process that involves several cell types, each with different developmental origins. Owing to this complexity, the aetiology of CDH is not well understood. The pleuroperitoneal folds (PPFs) and the posthepatic mesenchymal plate (PHMP) are transient structures that are essential during diaphragm development. Using several mouse models, including lineage tracing, we demonstrate the heterogeneous nature of the cells that make up the PPFs. The conditional deletion of Wilms tumor 1 homolog (Wt1) in the non-muscle mesenchyme of the PPFs results in CDH. We show that the fusion of the PPFs and the PHMP to form a continuous band of tissue involves movements of cells from both sources. The PPFs of mutant mice fail to fuse with the PHMP and exhibit increased RALDH2 (also known as ALDH1A2) expression. However, no changes in the expression of genes (including Snai1, Snai2, Cdh1 and Vim) implicated in epithelial-to-mesenchymal transition are observed. Additionally, the mutant PPFs lack migrating myoblasts and muscle connective tissue fibroblasts (TCF4+/GATA4+), suggesting possible interactions between these cell types. Our study demonstrates the importance of the non-muscle mesenchyme in development of the diaphragm.
The darkness and the light: diurnal rodent models for seasonal affective disorder
Authors: Shankar Anusha , Williams Cory T. 
Published: January 2021

ABSTRACTThe development of animal models is a critical step for exploring the underlying pathophysiological mechanisms of major affective disorders and for evaluating potential therapeutic approaches. Although most neuropsychiatric research is performed on nocturnal rodents, differences in how diurnal and nocturnal animals respond to changing photoperiods, combined with a possible link between circadian rhythm disruption and affective disorders, has led to a call for the development of diurnal animal models. The need for diurnal models is most clear for seasonal affective disorder (SAD), a widespread recurrent depressive disorder that is linked to exposure to short photoperiods. Here, we briefly review what is known regarding the etiology of SAD and then examine progress in developing appropriate diurnal rodent models. Although circadian disruption is often invoked as a key contributor to SAD, a mechanistic understanding of how misalignment between endogenous circadian physiology and daily environmental rhythms affects mood is lacking. Diurnal rodents show promise as models of SAD, as changes in affective-like behaviors are induced in response to short photoperiods or dim-light conditions, and symptoms can be ameliorated by brief exposure to intervals of bright light coincident with activity onset. One exciting avenue of research involves the orexinergic system, which regulates functions that are disturbed in SAD, including sleep cycles, the reward system, feeding behavior, monoaminergic neurotransmission and hippocampal neurogenesis. However, although diurnal models make intuitive sense for the study of SAD and are more likely to mimic circadian disruption, their utility is currently hampered by a lack of genomic resources needed for the molecular interrogation of potential mechanisms.
Conjugate Addition of Acetal-Derived Benzyl Radicals Generated from Low-Valent Titanium-Mediated C–O Bond Cleavage
Authors: Suga Takuya , Nakamura Masaharu , Takada Ryusei , Ukaji Yutaka 
Published: January 2021

Abstract
A new method for the generation of benzyl radicals from acetals via low-valent titanium-mediated homolytic C–O bond cleavage is presented. The low cost and availability of the developed titanium reagent enable efficient access to α-alkoxy carbon radical species via the developed reaction.
Iliopsoas Abscess
Authors: Sobaih Bader , Sobaih Loay , Zamil Fahad Al 
Published: January 2021

Iliopsoas abscess (IPA) is uncommon condition in children, diagnosis might be delayed because of nonspecific signs and symptoms. Only few patients have classical clinical triad at presentation in the form of fever, back pain, and inguinal pain at hip flexion. The diagnosis most likely to be reached in the first time by the use of abdominal computed tomography (CT) scan. We present a Saudi child with nonspecific signs and symptoms of fever, flank pain, and limping who was diagnosed as IPA by abdominal ultrasound and CT scan. The case was managed with intravenous antibiotics along with transcutaneous abscess drainage.
doi: https://doi.org/10.12669/pjms.37.2.3816
How to cite this:Sobaih B, Sobaih L, Al-Zamil F. Iliopsoas Abscess. Pak J Med Sci. 2021;37(2):605-607. doi: https://doi.org/10.12669/pjms.37.2.3816
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Agresja Federacji Rosyjskiej na Ukrainie – skutki polityczne, prawne i gospodarcze
Authors: Kopijka Walerij , Perepełycia Grigoryj 
Published: January 2021

Russian Aggression Against Ukraine: Political, International – Legal And Economic Consequences
Russian military aggression against Ukraine was the culmination of inter-civilizational confrontation of Russia with the West for the final consolidation of spheres of influence in Europe and the world in terms of transformation of international relations from a unipolar to a multipolar system. An important interest for Russia in this confrontation is to return it to the status of a world power. Revolutionary changes in Ukrainian society led to democratization, desovietization and consolidation of the Ukrainian nation. The immediate reaction on this revolutionary process was the Russian military aggression against Ukraine. The military aggression against Ukraine was carried out in the form of a hybrid war. The basic composition of Russian hybrid war in Donbas was inspired internal conflict in Ukraine between the center and the separatist regions. The distinction of Donbas hybrid version of the war is to use armed terror, high intensity combat operations, maneuver their character and widespread use of heavy weapons fire power. The annexation of the Crimea and the occupation of Donbas by Russian troops have a number of consequences that negatively affect international relations and modern system of international law. Aggression of leading to the erosion of the foundations of world order and international security, which could lead to the destruction of the existing world order.
Parametric sideband generation in CMOS-compatible oscillators from visible to telecom wavelengths
Authors: Domeneguetti Renato R. , Zhao Yun , Ji Xingchen , Martinelli Marcelo , Lipson Michal , Gaeta Alexander L. , Nussenzveig Paulo 
Published: January 2021

We present an approach for generating widely separated first sidebands based solely on the four-wave-mixing process in optical parametric oscillators built on complementary metal–oxide–semiconductor-compatible photonic chips. Using higher-order transverse modes to perform dispersion engineering, we obtain zero-group-velocity dispersion near 796 nm. By pumping the chip in the normal dispersion region, at 795.6 nm, we generate a signal field in the visible band (at 546.2 nm) and the corresponding idler field in the telecom band (at 1465.3 nm), corresponding to a frequency span of approximately 346 THz. We show that the spectral position of signal and idler can be tailored by exploiting a delicate balance between second- and fourth-order dispersion terms. Furthermore, we explicitly demonstrate a change in the parametric oscillation dynamics when moving the pump field from the anomalous to normal dispersion, where the chip ceases producing multiple sidebands adjacent to the pump field and generates widely separated single sidebands. This provides a chip-scale platform for generating single-sideband fields separated by more than one octave, covering the visible and telecom spectral regions.
Does perfusion computed tomography correlate to pathology in colorectal liver metastases?
Authors: van Amerongen M. J. , Vos A. M. , van der Woude W. , Nagtegaal I. D. , de Wilt J. H. W. , Fütterer J. J. , Hermans J. J. 
Published: January 2021

Introduction
Targeted therapy against tumor angiogenesis is widely used in clinical practice for patients with colorectal liver metastases (CRLM). Possible predictive biomarkers for tumor angiogenesis, such as, microvessel density (MVD), hypoxia and cell proliferation, can be determined using immunohistochemical staining. However, patients ineligible for surgical treatment need to undergo invasive diagnostic interventions in order to determine these biomarkers. CT perfusion (CTP) is an emerging functional imaging technique, which can non-invasively determine vascular properties of solid tumors. The purpose of this study was to evaluate CTP with histological biomarkers in CRLM.

Material and methods
Patients with CRLM underwent CTP one day before liver surgery. CTP analysis was performed on the entire volume of the largest metastases in each patient. Dual-input maximum slope analysis was used and data concerning arterial flow (AF), portal flow (PF) and perfusion index (PI) were recorded. Immunohistochemical staining with CD34, M75/CA-IX and MIB-1 was performed on the rim in the midsection of the tumor to determine respectively MVD, hypoxia and cell proliferation.

Results
Twenty CRLM in 20 patients were studied. Mean size of the largest CRLM was 37 mm (95% CI 21–54 mm). Mean AF and PF were respectively 64 ml/min/100ml (95% CI 48–79) and 30 ml/min/100ml (95% CI 22–38). Mean PI was 68% (95% CI 62–73). No significant correlation was found between tumor growth patterns and CTP (p = 0.95). MVD did not significantly correlate to AF (r = 0.05; p = 0.84), PF (r = 0.17; p = 0.47) and PI (r = -0.12; p = 0.63). Cell proliferation also did not significantly correlate to AF (r = 0.07; p = 0.78), PF (r = -0.01; p = 0.95) and PI (r = 0.15; p = 0.52). Hypoxia did not significantly correlate to AF (r = -0.05; p = 0.83), however, significantly to PF (r = 0.51; p = 0.02) and a trend to negative correlation with PF (r = -0.43; p = 0.06). However, after controlling the false discovery rate, no significant correlation between CTP and used immunohistochemical biomarkers was found.

Conclusion
In conclusion, this feasibility study found a trend to negative correlation between PI and hypoxia, CTP might therefore possibly evaluate this prognostic marker in CRLM non-invasively. However, CTP is not an appropriate technique for the assessment of microvessels or cell proliferation in CRLM.
High genetic variability of Schmallenberg virus M-segment leads to efficient immune escape from neutralizing antibodies
Authors: Wernike Kerstin , Reimann Ilona , Banyard Ashley C. , Kraatz Franziska , La Rocca S. Anna , Hoffmann Bernd , McGowan Sarah , Hechinger Silke , Choudhury Bhudipa , Aebischer Andrea , Steinbach Falko , Beer Martin 
Published: January 2021

Schmallenberg virus (SBV) is the cause of severe fetal malformations when immunologically naïve pregnant ruminants are infected. In those malformed fetuses, a “hot-spot”-region of high genetic variability within the N-terminal region of the viral envelope protein Gc has been observed previously, and this region co-localizes with a known key immunogenic domain. We studied a series of M-segments of those SBV variants from malformed fetuses with point mutations, insertions or large in-frame deletions of up to 612 nucleotides. Furthermore, a unique cell-culture isolate from a malformed fetus with large in-frame deletions within the M-segment was analyzed.
Each Gc-protein with amino acid deletions within the “hot spot” of mutations failed to react with any neutralizing anti-SBV monoclonal antibodies or a domain specific antiserum. In addition, in vitro virus replication of the natural deletion variant could not be markedly reduced by neutralizing monoclonal antibodies or antisera from the field. The large-deletion variant of SBV that could be isolated in cell culture was highly attenuated with an impaired in vivo replication following the inoculation of sheep.
In conclusion, the observed amino acid sequence mutations within the N-terminal main immunogenic domain of glycoprotein Gc result in an efficient immune evasion from neutralizing antibodies in the special environment of a developing fetus. These SBV-variants were never detected as circulating viruses, and therefore should be considered to be dead-end virus variants, which are not able to spread further. The observations described here may be transferred to other orthobunyaviruses, particularly those of the Simbu serogroup that have been shown to infect fetuses. Importantly, such mutant strains should not be included in attempts to trace the spatial-temporal evolution of orthobunyaviruses in molecular-epidemiolocal approaches during outbreak investigations.
Representation of Contralateral Visual Space in the Human Hippocampus
Authors: Silson Edward H. , Zeidman Peter , Knapen Tomas , Baker Chris I. 
Published: January 2021

The initial encoding of visual information primarily from the contralateral visual field is a fundamental organizing principle of the primate visual system. Recently, the presence of such retinotopic sensitivity has been shown to extend well beyond early visual cortex to regions not historically considered retinotopically sensitive. In particular, human scene-selective regions in parahippocampal and medial parietal cortex exhibit prominent biases for the contralateral visual field. Here, we used fMRI to test the hypothesis that the human hippocampus, which is thought to be anatomically connected with these scene-selective regions, would also exhibit a biased representation of contralateral visual space. First, population receptive field (pRF) mapping with scene stimuli revealed strong biases for the contralateral visual field in bilateral hippocampus. Second, the distribution of retinotopic sensitivity suggested a more prominent representation in anterior medial portions of the hippocampus. Finally, the contralateral bias was confirmed in independent data taken from the Human Connectome Project (HCP) initiative. The presence of contralateral biases in the hippocampus, a structure considered by many as the apex of the visual hierarchy, highlights the truly pervasive influence of retinotopy. Moreover, this finding has important implications for understanding how visual information relates to the allocentric global spatial representations known to be encoded therein.SIGNIFICANCE STATEMENTRetinotopic encoding of visual information is an organizing principle of visual cortex. Recent work demonstrates this sensitivity in structures far beyond early visual cortex, including those anatomically connected to the hippocampus. Here, using population receptive field (pRF) modeling in two independent sets of data we demonstrate a consistent bias for the contralateral visual field in bilateral hippocampus. Such a bias highlights the truly pervasive influence of retinotopy, with important implications for understanding how the presence of retinotopy relates to more allocentric spatial representations.
An Anticipatory Circuit Modification That Modifies Subsequent Task Switching
Authors: Wang Yanqing , Barry Michael A. , Cambi Monica , Weiss Klaudiusz R. , Cropper Elizabeth C. 
Published: January 2021

Modulators are generally expected to establish a network configuration that is appropriate for the current circumstances. We characterize a situation where the opposite is apparently observed. A network effect of a peptide modulator is counterproductive in that it tends to impede rather than promote the creation of the configuration that is appropriate when the modulator is released. This raises a question: why does release occur? We present data that strongly suggest that it impacts task switching. Our experiments were conducted in anAplysiafeeding network that generates egestive and ingestive motor programs. Initial experiments focused on egestive activity and the neuron B8. As activity becomes egestive, there is an increase in synaptic drive to B8 and its firing frequency increases (Wang et al., 2019). We show that, as this occurs, there is also a persistent current that develops in B8 that is outward rather than inward. Dynamic clamp introduction of this current decreases excitability. When there is an egestive-ingestive task switch inAplysia, negative biasing is observed (i.e., a bout of egestive activity has a negative impact on a subsequent attempt to initiate an ingestive response) (Proekt et al., 2004). Using anin vitroanalog of negative biasing, we demonstrate that the outward current that develops during egestive priming plays an important role in establishing this phenomenon. Our data suggest that, although the outward current induced as activity becomes egestive is counterproductive at the time, it plays an anticipatory role in that it subsequently impacts task switching.SIGNIFICANCE STATEMENTIn this study, we identify a peptide-induced circuit modification (induction of an outward current) that does not immediately promote the establishment of a behaviorally appropriate network configuration. We ask why this might occur, and present data that strongly suggest that it plays an important role during task switching. Specifically, our data suggest that the outward current we characterize plays a role in the negative biasing that is seen in the molluscAplysiawhen there is a transition from egestive to ingestive activity. It is possible that the mechanism that we describe operates in other species. A negative effect of egestion on subsequent ingestion is observed throughout the animal kingdom.
Microorganisms causing respiratory diseases in children in relation to age and diagnosis
Authors: Isaieva H. O. , Mishyna M. M. , Gonchar M. O. , Logvinova O. L. , Basiuk M. A. 
Published: January 2021

Community-acquired pneumonia (CAP) in children is still one of the leading causes of morbidity and mortality, especially in developing countries. The World Health Organization (WHO) has reported that pneumonia accounts for 15% of all deaths of children under 5 years old. The aim of the present study was to find out the predominance of microorganisms in the respiratory tract in children. 334 strains of microorganisms were isolated: Gram-positive – 293 strains, Gram-negative – 41. From the pharynx 183 strains were isolated, from the nose – 94, from sputum – 57. Among Gram-positive microorganisms the following were isolated: Staphylococcus aureus 44 strains of microorganisms, S. epidermidis – 75, Group A β-hemolytic streptococci – 39, viridans streptococci – 55, Streptococcus pneumoniae – 34, Enterococcus faecalis – 2, Candida spp. – 38, Corynebacterium pseudodiphthericum – 6. Among Gram-negative microorganisms the following were isolated: Escherichia coli 4 strains of microorganisms, Klebsiella pneumoniae – 13, Pseudomonas aeruginosa – 6, Haemophilus influenzae – 11, Enterobacter cloacae – 7. Children were divided by age and diagnosis into four groups: I group – children with acute bronchitis (0–5 years of age), II group – children with acute bronchitis (5–18 years of age), III group – children with CAP (0–5 years of age), IV group – children with CAP (5–18 years of age). Materials used in the research – nasal swabs, throat swabs and sputum. Microorganisms were isolated and identified using standard microbiological methods. S. aureus was the predominant microorganism isolate from the pharynx in children with bronchitis (0–5 years). Group A β-hemolytic streptococci were isolated most often from the pharynx in children with bronchitis older than 5 years and in children with CAP less than 5 years of age. S. pneumoniae was most often isolated from the pharynx in children older than 5 years of age with CAP. S. aureus was the main microorganism, isolated from the nose in children with bronchitis in all age groups; in patients with CAP it was the predominant microorganism in children older than 5 years of age. S. aureus was the predominant microorganism, isolated from sputum in children with bronchitis older than 5 years. S. pneumoniae was the predominant microorganism, isolated from sputum in children with CAP older than 5 years. The research showed that S. pneumoniae is still one of the main pathogens that cause CAP in school aged children.