bluuebunny/crossref_metadata_2025_split huggingface dataset:
Kontinuitätserhalt des Nervus cochlearis bei der retrosigmoidalen ablativen Osteotomie des inneren Gehörgangs bei fortgeschrittenen Vestibularisschwannomen
Authors: Schaumann Katharina , Albrecht A. , Turowski B. , Hoffmann C. , Cornelius J. F. , Schipper J. 
Published: November 2021

ZusammenfassungAusgewertet wurden 86 Patienten mit einem retrosigmoidal mikrochirurgisch resezierten Vestibularisschwannom im Tumorstadium Koos II–IV. Es zeigte sich, dass im Bereich des inneren Gehörgangs in über 2/3 der Fälle der Nervus cochlearis dem elektroneurographisch leicht zu identifizierenden Nervus facialis in immer wiederkehrenden ähnlichen Verlaufsmustern folgte. Ausgehend vom Fundus erleichterte dies die frühzeitige Identifizierung und damit den Kontinuitätserhalt des Nervus cochlearis im Verlauf des inneren Gehörgangs. Dies war vor allem dann von Bedeutung, wenn ein sicherer Funktionserhalt aufgrund der Tumorgröße oder -formation trotz intraoperativer Ableitung somatosensorischer Potenziale nicht sicher gewährleistet werden konnte, aber die Möglichkeit einer späteren Hörrehabilitation mit einem Cochleaimplantat bestehen bleiben sollte. Präoperative Magnetresonanztomographie(MRT)-Sequenzen ergaben zwar in einigen Fällen einen Hinweis auf die möglichen Nervenverläufe, die intraoperative Darstellung im inneren Gehörgang war der MRT aber überlegen.
A rapid magnetic bead-based immunoassay for sensitive determination of diclofenac
Authors: Ecke Alexander , Westphalen Tanja , Hornung Jane , Voetz Michael , Schneider Rudolf J. 
Published: November 2021

Abstract
Increasing contamination of environmental waters with pharmaceuticals represents an emerging threat for the drinking water quality and safety. In this regard, fast and reliable analytical methods are required to allow quick countermeasures in case of contamination. Here, we report the development of a magnetic bead-based immunoassay (MBBA) for the fast and cost-effective determination of the analgesic diclofenac (DCF) in water samples, based on diclofenac-coupled magnetic beads and a robust monoclonal anti-DCF antibody. A novel synthetic strategy for preparation of the beads resulted in an assay that enabled for the determination of diclofenac with a significantly lower limit of detection (400 ng/L) than the respective enzyme-linked immunosorbent assay (ELISA). With shorter incubation times and only one manual washing step required, the assay demands for remarkably shorter time to result (< 45 min) and less equipment than ELISA. Evaluation of assay precision and accuracy with a series of spiked water samples yielded results with low to moderate intra- and inter-assay variations and in good agreement with LC–MS/MS reference analysis. The assay principle can be transferred to other, e.g., microfluidic, formats, as well as applied to other analytes and may replace ELISA as the standard immunochemical method.
Graphical abstract
The architecture of Recent brachiopod shells: diversity of biocrystal and biopolymer assemblages in rhynchonellide, terebratulide, thecideide and craniide shells
Authors: Simonet Roda Maria , Griesshaber Erika , Angiolini Lucia , Rollion-Bard Claire , Harper Elizabeth M. , Bitner Maria Aleksandra , Milner Garcia Sara , Ye Facheng , Henkel Daniela , Häussermann Vreni , Eisenhauer Anton , Gnägi Helmut , Brand Uwe , Logan Alan , Schmahl Wolfgang W. 
Published: November 2021

AbstractBiological hard tissues are a rich source of design concepts for the generation of advanced materials. They represent the most important library of information on the evolution of life and its environmental conditions. Organisms produce soft and hard tissues in a bottom-up process, a construction principle that is intrinsic to biologically secreted materials. This process emerged early on in the geological record, with the onset of biological mineralization. The phylum Brachiopoda is a marine animal group that has an excellent and continuous fossil record from the early Cambrian to the Recent. Throughout this time interval, the Brachiopoda secreted phosphate and carbonate shells and populated many and highly diverse marine habitats. This required great flexibility in the adaptation of soft and hard tissues to the different marine environments and living conditions. This review presents, juxtaposes and discusses the main modes of mineral and biopolymer organization in Recent, carbonate shell-producing, brachiopods. We describe shell tissue characteristics for taxa of the orders Rhynchonellida, Terebratulida, Thecideida and Craniida. We highlight modes of calcite and organic matrix assembly at the macro-, micro-, and nano-scales based on results obtained by Electron Backscatter Diffraction, Atomic Force Microscopy, Field Emission Scanning Electron Microscopy and Scanning Transmission Electron Microscopy. We show variation in composite hard tissue organization for taxa with different lifestyles, visualize nanometer-scale calcite assemblies for rhynchonellide and terebratulide fibers, highlight thecideide shell microstructure, texture and chemistry characteristics, and discuss the feasibility to use thecideide shells as archives of proxies for paleoenvironment and paleoclimate reconstructions.
The short and long-term implications of warming and increased sea water pCO2 on the physiological response of a temperate neogastropod species
Authors: Mardones Maria Loreto , Thatje Sven , Fenberg Phillip B. , Hauton Chris 
Published: November 2021

AbstractGlobal average temperatures and seawater pCO2 have rapidly increased due to the oceanic uptake of atmospheric carbon dioxide producing severe consequences for a broad range of species. The impacts on marine ectotherms have been largely reported at short-term scales (i.e. from days to weeks); however, the prolonged effects on long-term processes such as reproduction have received little attention. The gastropod Ocenebra erinaceus is a key predator structuring communities on rocky shores of the French and UK coasts. Even though rocky shore species are regarded as being very tolerant to changes in temperature and pH, many of them are living near their upper tolerance limits, making them susceptible to rapid environmental changes. Here, we report that future mean seawater conditions (RCP8.5, + 3 °C and ~ 900 μatm CO2) do not significantly affect the physiology and molecular response of O. erinaceus adults after 132 days. During the first 50 days, there was a slight impact on oxygen consumption rates and body weight; however, after 95 days of exposure, gastropods fully acclimated to the experimental condition. Despite this, reproduction in females exposed to these future seawater conditions ceased after long-term exposure (~ 10 months). Therefore, in the short-term, O. erinaceus appear to be capable of full compensation; however, in the long-term, they fail to invest in reproduction. We conclude studies should be based on combined results from both short- and long-term effects, to present realistic projections of the ecological consequences of climate warming.
Endoplasmic reticulum stress-induced release and binding of calreticulin from human ovarian cancer cells
Authors: Abdullah Trefa M. , Whatmore Jacqueline , Bremer Edwin , Slibinskas Rimantas , Michalak Marek , Eggleton Paul 
Published: November 2021

Abstract
Background
Calreticulin (CRT) is an endoplasmic reticulum (ER) chaperone, but can appear surface bound on cancers cells, including ovarian cancers (OC). We investigated at what stage of cell viability, CRT appeared associated with surface of human OC cells. CRT on pre-apoptotic tumour cells is thought to initiate their eradication via a process termed immunogenic cell death (ICD).

Methods
We treated OC cells with the chemotherapeutic—doxorubicin (DX) known to induce translocation of CRT to some tumour cell surfaces, with and without the ER stressor—thapsigargin (TG)—and/or an ER stress inhibitor—TUDCA. We monitored translocation/release of CRT in pre-apoptotic cells by flow cytometry, immunoblotting and ELISA. We investigated the difference in binding of FITC-CRT to pre-apoptotic, apoptotic and necrotic cells and the ability of extracellular CRT to generate immature dendritic cells from THP-1 monocytes.

Results
Dx-treatment increased endogenously released CRT and extracellular FITC_CRT binding to human pre-apoptotic OC cells. DX and TG also promoted cell death in OC cells which also increased CRT release. These cellular responses were significantly inhibited by TUDCA, suggesting that ER stress is partially responsible for the changes in CRT cellular distribution. Extracellular CRT induces maturation of THP-1 towards a imDC phenotype, an important component of ICD.

Conclusion
Collectively, these cellular responses suggest that ER stress is partially responsible for the changes in CRT cellular distribution. ER-stress regulates in part the release and binding of CRT to human OC cells where it may play a role in ICD.
Digitalised exercise material in forensic odontology
Authors: Knivsberg Ina C. , Kopperud Simen E. , Bjørk Mai-Britt , Torgersen Gerald , Skramstad Katarzyna , Kvaal Sigrid I. 
Published: November 2021

Abstract
Introduction
This paper presents digital educational material in forensic odontology, including dental identification after multiple fatalities and dental age estimation from different age groups.

Material and method
Electronic patient records consisting of intraoral scans of the dentition, digital radiographs, photographs and written dental records were collected. Exercises in age estimations contained digital radiographs and photographs of ground tooth sections, with digital measuring tools and tables according to age groups. The teaching material was organised as a module in an electronic Learning Management System with external links to all relevant teaching material.

Results
For the identification exercises, intraoral scans and the latest digital radiographs simulated the postmortem examination of the deceased. For comparison, all other radiographs, photographs and dental records were available as antemortem material. The exercise was to match postmortem findings with the antemortem records using the Interpol standard and reconciliation. Age assessment of children used designated tables to grade tooth development on digital radiographs. For adults, non-destructive methods, digital radiographs, photographs and measuring tools were used.

Discussion
The teaching concept was hybrid, but it can easily be adapted as a fully digital exercise. The instructions and written material can be translated into different languages. The level of difficulty in the exercises can be adjusted according to the participant’s level of knowledge.

Conclusion
The educational material embraces the new possibilities for digitalisation and intraoral scanning. This might be a valuable tool for motivating and engaging the students in their participation and understanding of the subject.
ROS-induced oxidative stress in plant cryopreservation: occurrence and alleviation
Authors: Ren Li , Wang Min-Rui , Wang Qiao-Chun 
Published: November 2021

Abstract
Main conclusion
Reactive oxygen species (ROS)-induced oxidative stress results in low success or even total failure of cryopreservation. Better understanding of how the plant establishes resistance/tolerance to ROS-induced oxidative stress facilitates developments of robust cryopreservation procedures.

Abstract
Cryopreservation provides a safe and efficient strategy for long-term preservation of plant genetic resources. ROS-induced oxidative stress caused damage to cells and reduced the ability of the plant to survive following cryopreservation, eventually resulting in low success or even total failure. This paper provides updated and comprehensive information obtained in the past decade, including the following: (1) ROS generations and adaptive responses of antioxidant systems during cryopreservation; (2) expressions of oxidative stress-associated genes and proteins during cryopreservation; (3) ROS-triggered programmed cell death (PCD) during cryopreservation; and (4) exogenous applications of enzymatic and non-enzymatic antioxidants in improving success of cryopreservation. Prospects for further studies are proposed. The goal of the present study was to facilitate better understanding of the mechanisms by which the plant establishes resistance/tolerance to oxidative stress during cryopreservation and promote further studies toward the developments of robust cryopreservation procedures and wider application of plant cryobiotechnology.
Structural brain abnormalities in children and young adults with severe chronic kidney disease
Authors: Lijdsman Sophie , Königs Marsh , van Sandwijk Marit S. , Bouts Antonia H. , van Hoeck Koen , de Jong Huib , Engelen Marc , Oosterlaan Jaap , Bemelman Frederike J. , Oostrom Kim J. , Groothoff Jaap W. 
Published: November 2021

Abstract
Background
The pathophysiology of neurological dysfunction in severe chronic kidney disease (CKD) in children and young adults is largely unknown. We aimed to investigate brain volumes and white matter integrity in this population and explore brain structure under different treatment modalities.

Methods
This cross-sectional study includes 24 patients with severe CKD (eGFR < 30) aged 8–30 years (median = 18.5, range = 9.1–30.5) on different therapy modalities (pre-dialysis, n = 7; dialysis, n = 7; transplanted, n = 10) and 21 healthy controls matched for age, sex, and parental educational level. Neuroimaging targeted brain volume using volumetric analysis on T1 scans and white matter integrity with tract-based spatial statistics and voxel-wise regression on diffusion tensor imaging (DTI) data.

Results
CKD patients had lower white matter integrity in a widespread cluster of primarily distal white matter tracts compared to healthy controls. Furthermore, CKD patients had smaller volume of the nucleus accumbens relative to healthy controls, while no evidence was found for abnormal volumes of gray and white matter or other subcortical structures. Longer time since successful transplantation was related to lower white matter integrity. Exploratory analyses comparing treatment subgroups suggest lower white matter integrity and smaller volume of the nucleus accumbens in dialysis and transplanted patients relative to healthy controls.

Conclusions
Young CKD patients seem at risk for widespread disruption of white matter integrity and to some extent smaller subcortical volume (i.e., nucleus accumbens). Especially patients on dialysis therapy and patients who received a kidney transplant may be at risk for disruption of white matter integrity and smaller volume of the nucleus accumbens.

Graphical abstract
Radiation Medical Countermeasures and Use of EPR Biodosimetry to Facilitate Effectiveness of Applied Clinical Procedures
Authors: Singh Vijay K. , Swartz Harold M. , Seed Thomas M. 
Published: November 2021

AbstractThe utility for electron paramagentic resonance (EPR or ESR)-based radiation biodosimetry has received increasing recognition concerning its potential to assist in guiding the clinical management of medical countermeasures in individuals unwantedly exposed to injurious levels of ionizing radiation. Similar to any of the standard physical dosimetric methods currently employed for screening clinically significant radiation exposures, the EPR-based in vivo dosimetry approach would serve to complement and extend clinical assessments (e.g., blood analyses, cytogenetics, etc.), specifically to more accurately assign the extent of ionizing radiation exposure that individuals might have received. In the case of EPR biodosimetry of biological samples such as nails, teeth, and bones, the method has the capability of providing information on the physical dose at several specific bodily sites and perhaps additonal information on the homogeneity of the exposure as well as its overall magnitude. This information on radiation dose and distribution would be of significant value in providing medical management to given individuals at health risk due to radiation exposure. As these measurements provide information solely on physical measures of the radiation dose and not on the potential biological impact of a particular dose, they are complementary, albeit supplemental, to the array of currently available biologically based biodosimetry and clinical findings. In aggregate, these physical and biological measures of radiation exposure levels (dose) would most certainly provide additional, useful information for the effective medical management of radiation exposed individuals.
SPIDE: sybil-proof, incentivized data exchange
Authors: Skowroński Rafał , Brzeziński Jerzy 
Published: November 2021

AbstractDecentralized, open-access blockchain systems opened up new, exciting possibilities—all without reliance on trusted third parties. Regardless of the employed consensus protocol, the overall security, decentralization and effectiveness of such systems, largely depend upon properly structured incentives. Indeed, as has been previously spotted by Babaiaff et al. Bitcoin-like systems, oftentimes lack some of these. Specifically, current blockchain-systems fail to incentivize one of their crucial aspects–the underlying data exchange. As we rationalize, proper incentivization of that layer could lead to lower transactions’ confirmation-times, improved finalization guarantees and at the same time to discouragement of malicious behaviours such as block-withholding attacks. Indeed, incentivization of the data-exchange layer allows the system to remain operational when all agents, including routing nodes, are assumed to be rational. In this work, while focusing on the problem of sybil-proof data exchange, we revisit previous approaches, showcasing their shortcomings and lay forward the first information exchange framework; with integrated routing and reward-function mechanics, provably secure in thwarting Sybil-nodes in 1-connected or eclipsed networks. The framework neither requires nor assumes any kind of constraints in regard to the network’s topology (i.e. the network is modelled as a random-connected graph) and rewards information propagators through a system-intrinsic virtual asset maintained by the decentralized state-machine. The proposal, while being storage and transmission efficient is suitable for rewarding not only consensus-related datagrams (both data-blocks and transactions) but consensus-extrinsic information as well, thus facilitating an universal sybil-proof data-exchange apparatus, provably valid under the assumption of existence of a data store whose property of non-malleability emerges as time approaches infinity. Our research was conducted under two scenarios—with round leader known and unknown in advance of each transactional round.
Characterization of polycyclic aromatic hydrocarbons in the Great Lakes Basin using dreissenid mussels
Authors: Kimbrough Kimani , Jacob Annie , Regan Seann , Davenport Erik , Edwards Michael , Leight A. K. , Freitag Amy , Rider Mary , Johnson W. Edward 
Published: November 2021

AbstractThe National Oceanic and Atmospheric Administration (NOAA), National Centers for Coastal Ocean Science (NCCOS) Mussel Watch Program (MWP), conducts basin-wide monitoring and place-based assessments using dreissenid mussels as bioindicators of chemical contamination in the Laurentian Great Lakes. Polycyclic aromatic hydrocarbons (PAHs) body burden results for the period 2009–2018 were combined into one dataset from multiple MWP studies allowing for a robust characterization of PAH contamination. Patterns in PAH data were identified using descriptive statistics and machine learning techniques. Relationships between total PAH concentration in dreissenid mussel tissue, impervious surface percentages, and PAH relative concentration were identified and used to build a predictive model for the Great Lakes Basin. Significant positive correlation was identified by the Spearman’s rank correlation test between total PAH concentration and percent impervious surface. The findings support the paradigm that PAHs are primarily derived from land-based sources. Offshore and riverine locations had the lowest and highest median total PAH concentrations, respectively. PAH assemblages and ratios indicated that pyrogenic sources were more predominant than petrogenic sources and that PAHs at offshore sites exhibited relatively more weathering compared to inshore sites.
A Confirmation Bias View on Social Media Induced Polarisation During Covid-19
Authors: Modgil Sachin , Singh Rohit Kumar , Gupta Shivam , Dennehy Denis 
Published: November 2021

AbstractSocial media has played a pivotal role in polarising views on politics, climate change, and more recently, the Covid-19 pandemic. Social media induced polarisation (SMIP) poses serious challenges to society as it could enable ‘digital wildfires’ that can wreak havoc worldwide. While the effects of SMIP have been extensively studied, there is limited understanding of the interplay between two key components of this phenomenon: confirmation bias (reinforcing one’s attitudes and beliefs) and echo chambers (i.e., hear their own voice). This paper addresses this knowledge deficit by exploring how manifestations of confirmation bias contributed to the development of ‘echo chambers’ at the height of the Covid-19 pandemic. Thematic analysis of data collected from 35 participants involved in supply chain information processing forms the basis of a conceptual model of SMIP and four key cross-cutting propositions emerging from the data that have implications for research and practice.
Developing a theory-based behavior change intervention to improve the prescription of surgical prophylaxis
Authors: Lohiniva Anna Leena , Heweidy Iman , Girgis Samiha , Abouelata Omar , Ackley Caroline , Samir Shady , Talaat Maha 
Published: November 2021

AbstractBackgroundAntimicrobial resistance (AMR) is increasingly pervasive due to multiple, complex prescribing and consuming behaviours. Accordingly, behaviour change is an important component of response to AMR. Little is known about the best approaches to change antibiotic use practices and behaviours.AimThis project aims to develop a context-specific behaviour change strategy focusing on promoting appropriate prescription practices following the World Health Organization recommendations for surgical prophylaxis in an orthopaedic surgery unit in Egypt.MethodThe project included a formative qualitative research study with 31 in-depth interviews with orthopaedic surgeons that was based on the Theoretical Domains Framework (TDF) and an intervention that was developed to following the Behaviour Change Wheel (BCW) in a knowledge co-production workshop with ten public health experts that ensured that the theory based intervention was a culturally acceptable, practical and implementable intervention.ResultsThe prescription of surgical prophylaxis was influenced by eight TDF domains from which workshop participants selected five to be included in the behaviour change intervention including, knowledge, belief in consequences (mistrust towards infection prevention and control measures), environmental factors (lack of prescription guidelines), professional role and reinforcement (a lack of appropriate follow up actions influenced prescription of surgical prophylaxis). The appropriate set of behaviour change functions of BCW and related activities to improve the current practices included education, enablement, persuasion, environmental restructuring and restriction.ConclusionThe study showed that a theory based, and context specific intervention can be created by using the TDF and BCW together with knowledge-co creation to improve the prescription of surgical prophylaxis in and Egyptian orthopaedic unit. The intervention needs to piloted and scaled up.