bluuebunny/crossref_metadata_2025_split huggingface dataset:
Promoting physical activity and health in Hong Kong primary school children through a blended physical literacy intervention: protocol and baseline characteristics of the “Stand+Move” randomized controlled trial
Authors: Li Ming Hui , Sit Cindy Hui Ping , Wong Stephen Heung Sang , Wing Yun Kwok , Ng Ching Kong , Sum Raymond Kim Wai 
Published: December 2021

Abstract
Background
Children predominantly remain sedentary in a traditional classroom. This study aimed to demonstrate the design and baseline characteristics of a three-armed intervention program that targeted enhancements in children’s physical literacy (PL), physical activity (PA), sleep, and executive functions by constructing an active classroom environment in primary schools. The blended approach involved organized PA participation during recess combined with changes to the classroom environment in response to the emphasis on nurturing children’s PL in Hong Kong.

Methods
This blended “Stand + Move” intervention adopted a randomized controlled trial design to investigate its effectiveness in improving health-related aspects. Three groups were compared: (1) PA recess intervention, (2) blended (sit-stand desks and PA recess) experimental, and (3) control groups. In this 13-week intervention (from March to September 2019), 76 students (59.2% girls) were recruited and randomly assigned to the three groups. The primary outcomes were children’s PL and PA. The secondary outcomes were children’s sleep and executive functions.

Results
Baseline data were evaluated. The mean age was 9.6 years [standard deviation = 0.61, range 9.0–12.0]. There were no significant differences between trial arms at baseline concerning any of the outcomes (all P = 0.06–0.89). Overall, 22.4% met the recommended PA guidelines, 36.8% met the sleep guidelines, and 10.5% met both guidelines. Three aspects of executive functions were evaluated: inhibition, executive control, and planning. Over half of the participants reported satisfaction with their perceived sleep quality.

Conclusions
The designed intervention is regarded as an innovative strategy that incorporates sit-stand desks and PA breaks to reconstruct children’s traditional classroom environment. The baseline results suggest that intervention was satisfactory in reducing students’ sitting time and increasing their PA engagement. We demonstrated the benefits of this intervention on children’s PL, various sleep patterns, and executive functions. As expected, the designed intervention changes made to the classroom improved children’s health behaviors, as well as the support from stakeholders at schools and the children’s families. Our results also provided the desired evidence for policy reforms in teaching and learning strategies.

Trial registration
ChiCTR ChiCTR2000035038. Registered on July 29, 2020—retrospectively registered
In-silico analysis reveals druggable single nucleotide polymorphisms in angiotensin 1 converting enzyme involved in the onset of blood pressure
Authors: Udosen Brenda , Soremekun Opeyemi , Ekenna Chinwe , Idowu Omotuyi Olaposi , Chikowore Tinashe , Nashiru Oyekanmi , Fatumo Segun 
Published: December 2021

Abstract
Objective
The Angiotensin 1 converting enzyme (ACE1) gene plays a critical role in regulating blood pressure and thus, it has become a major therapeutic target of antihypertensives. Single nucleotide polymorphisms (SNPs) occurring within a gene most especially at the functional segment of the genes alter the structure–function relationship of that gene.

Results
Our study revealed that five nsSNPs of the ACE1 gene were found to be potentially deleterious and damaging and they include rs2229839, rs14507892, rs12709442, and rs4977 at point mutations P351R, R953Q, I1018T, F1051V, and T1187M. The protein stability predictive tools revealed that all the nsSNPs decreased stability of the protein and the Consurf server which estimates the evolutionary conservation profile of a protein showed that three mutants were in the highly conserved region. In conclusion, this study predicted potential druggable deleterious mutants that can be further explored to understand the pathological basis of cardiovascular disease.
Impact of multisession 40Hz tACS on hippocampal perfusion in patients with Alzheimer’s disease
Authors: Sprugnoli Giulia , Munsch Fanny , Cappon Davide , Paciorek Rachel , Macone Joanna , Connor Ann , El Fakhri Georges , Salvador Ricardo , Ruffini Giulio , Donohoe Kevin , Shafi Mouhsin M. , Press Daniel , Alsop David C. , Pascual Leone Alvaro , Santarnecchi Emiliano 
Published: December 2021

Abstract
Background
Alzheimer’s disease (AD) is associated with alterations in cortical perfusion that correlate with cognitive impairment. Recently, neural activity in the gamma band has been identified as a driver of arteriolar vasomotion while, on the other hand, gamma activity induction on preclinical models of AD has been shown to promote protein clearance and cognitive protection.

Methods
In two open-label studies, we assessed the possibility to modulate cerebral perfusion in 15 mild to moderate AD participants via 40Hz (gamma) transcranial alternating current stimulation (tACS) administered 1 h daily for 2 or 4 weeks, primarily targeting the temporal lobe. Perfusion-sensitive MRI scans were acquired at baseline and right after the intervention, along with electrophysiological recording and cognitive assessments.

Results
No serious adverse effects were reported by any of the participants. Arterial spin labeling MRI revealed a significant increase in blood perfusion in the bilateral temporal lobes after the tACS treatment. Moreover, perfusion changes displayed a positive correlation with changes in episodic memory and spectral power changes in the gamma band.

Conclusions
Results suggest 40Hz tACS should be further investigated in larger placebo-controlled trials as a safe, non-invasive countermeasure to increase fast brain oscillatory activity and increase perfusion in critical brain areas in AD patients.

Trial registration
Studies were registered separately on ClinicalTrials.gov (NCT03290326, registered on September 21, 2017; NCT03412604, registered on January 26, 2018).
Optimal control for cooperative systems involving fractional Laplace operators
Authors: Serag H. M. , Hyder Abd-Allah , El-Badawy M. 
Published: December 2021

AbstractIn this work, the elliptic $2\times 2$
2
×
2
cooperative systems involving fractional Laplace operators are studied. Due to the nonlocality of the fractional Laplace operator, we reformulate the problem into a local problem by an extension problem. Then, the existence and uniqueness of the weak solution for these systems are proved. Hence, the existence and optimality conditions are deduced.
Weighted differential inequality and oscillatory properties of fourth order differential equations
Authors: Kalybay Aigerim , Oinarov Ryskul , Sultanaev Yaudat 
Published: December 2021

AbstractIn this paper, we investigate the oscillatory properties of two fourth order differential equations in dependence on boundary behavior of its coefficients at infinity. These properties are established based on two-sided estimates of the least constant of a certain weighted differential inequality.
Drastic advancement in nanophotonics achieved by a new dressed photon study
Authors: Sakuma Hirofumi , Ojima Izumi , Ohtsu Motoichi , Kawazoe Tadashi 
Published: December 2021

Abstract
On the very recent advancement of dressed photon studies
A timely review of the emerging new phase of dressed photon (DP) studies, not yet prevailing in the global nanophotonic society, is given in contradistinction to its preceding incipient phase. A new theory on DPs crucially depends on a couple of important elements, namely, the knowledge on quantum field theory (QFT) having infinite degrees of freedom, notably on the micro-macro duality (MMD) theory developed by Ojima, and a newly proposed Clebsch dual (CD) electromagnetic field as a specific implementation of MMD theory. The main aim of the first part of this article after the introduction, the section of “In search of refinement of the theoretical models”, is twofold: to explain plainly, without resorting to mathematical equations, the essence of the highly mathematical contents of MMD theory, which clarifies a problematic aspect of the Schrödinger’s cat thought experiment, and to explain the physical meanings of the CD field.

Preliminary study on the existence of DP light with spin zero
In the section of “Observed “photon cluster” and light field with spin zero”, we briefly report a new intriguing experimental discovery implying the existence of propagating particle-like “quantum DP light” together with a conjecture on its possible theoretical explanation. A perspective on a variety of possible research directions for DPs is then briefly mentioned in the final section.
Dental fluorosis among people and livestock living on Gihaya Island in Lake Kivu, Rwanda
Authors: Habiyakare Theodore , Schurer Janna M. , Poole Barika , Murcott Susan , Migabo Basile , Mardochee Birori , Amuguni J. Hellen , Morgan John P. 
Published: December 2021

Abstract
Background
Dental
fluorosis is caused by prolonged exposure to excessive fluoride during the period of permanent tooth formation and is characterized by tooth discoloration, pitting, and loss of shape. Communities living near Lake Kivu in Western Rwanda exhibit a high prevalence of dental fluorosis; however, data on prevalence and risk factors are scarce.

Methods
This cross sectional, quantitative study used a One Health approach to investigate dental fluorosis prevalence among people and livestock and to measure fluoride content in the environment. In 2018, oral health examinations were conducted to assess the prevalence of fluorosis in children (aged 9 to 15 years), cattle and goats residing on Gihaya Island (Rwanda, East Africa). All children and cattle/goats meeting basic eligibility criteria (e.g., island residence) were invited to participate. Presence and severity of dental fluorosis was categorized according to the Dean’s Fluorosis Index. Samples of local foods, water, soil and grass were collected from communal sources and individual households and analyzed for fluoride content using standard laboratory techniques. Descriptive and binomial analyses (Fisher Exact Test) were used to assess this dataset.

Results
Overall, 186 children and 85 livestock owners (providing data of 125 livestock -23 cattle and 102 goats) participated. Dental fluorosis was recorded in 90.7% of children and 76% of livestock. Moderate to severe fluorosis was observed in 77% children while goats and cattle most often exhibited mild or absent/questionable severity, respectively. Water from Lake Kivu (used primarily for human cooking water and livestock drinking water) contained fluoride levels that were consistently higher than the maximum threshold (1.5 mg/L) recommended by the World Health Organization. Other sources (borehole and rainwater) were within safe limits. All food, soil and grass samples contained fluoride. The highest levels were observed in porridge (0.5 mg/g) and small fishes (1.05 mg/g).

Conclusions
Altogether, dental fluorosis was highly prevalent among children and goats on Gihaya Island with various food and water sources contributing a cumulative exposure to fluoride. An immediate and coordinated response across human, animal and water professionals is needed to reduce fluoride exposure within safe limits for island residents.
Kounis syndrome secondary to nimesulide ingestion: a case report
Authors: Shrimanth Yamasandi Siddegowda , Vemuri Krishna Santosh , Gawalkar Atit A. , Ghosh Soumitra , Vijay Jyothi , Prarthana Thammannagowda , Sihag Bhupendra Kumar 
Published: December 2021

AbstractBackgroundKounis syndrome, also known as "allergic myocardial infarction," is a rare co-occurrence of acute coronary syndrome (ACS) in the setting of hypersensitivity reaction to any agent. Non-steroidal anti-inflammatory drugs (NSAIDs) like are often implicated in causing allergic reactions. Here, we present a case of anterior wall myocardial infarction (AWMI) occurred following angioedema secondary to intake of Nimesulide, not described earlier in literature.Case presentationA 45-year-old female developed generalized pruritic, erythematous maculopapular rash, facial puffiness, oral ulcers and hoarseness of voice within few hours following consumption of Nimesulide for fever and body-ache. Due to development of hypotension, electrocardiogram (ECG) was done, which revealed ST elevation in V2–V6, with marked elevation of troponin (TnI) and creatine kinase (CK-MB). He had no chest pain or shortness of breath. Echocardiography showed regional wall motion (RWMA) abnormality in left anterior descending artery (LAD) territory with an ejection fraction of 25%. Coronary angiography showed a complete thrombotic cutoff of LAD, for which Tirofiban infusion was started to decrease thrombus burden. Repeat angiography on next day showed 80% lesion in proximal LAD for which she underwent revascularization with a drug-eluting stent. The patient later showed improvement in cardiac function at 8 months of follow-up.ConclusionsThe occurrence of ACS requiring percutaneous coronary intervention (PCI) in the setting of allergic reactions is rarely reported in the literature. One should be aware of the rare possibility of Kounis syndrome in the setting of hypersensitivity reaction when accompanying features of symptoms suggestive of coronary artery disease co-exists. When indicated, ECG monitoring and cardiac biomarkers in patients with allergic responses help to identify this rare and treatable condition.
Effect of vascular endothelial growth factor gene polymorphisms on disease activity in rheumatoid arthritis
Authors: Sallam Rehab A. , Saad Bassant S. , El Wassefy Mona A. , EL Kady Basma A. 
Published: December 2021

Abstract
Background
The full etiology of RA remains unclear; in addition to the contributions of infectious, hormonal, and environmental factors, several lines of evidence have suggested that the disease has a genetic basis. The VEGF gene is also an independent risk factor for RA severity and correlates with multiple disease parameters, such as disease activity, joint damage, and functional disability. This case-control study aimed to investigate the impact of a common genetic polymorphism in the vascular endothelial growth factor (VEGF) gene on disease activity and synovial lesions in patients with rheumatoid arthritis (RA).

Results
T allele was present in the RA group more frequently (22.5% vs. 10% respectively in controls). The C allele was less frequent in the RA group (77.7% vs. 90% respectively in controls) (P = 0.002). Homozygous genotype (CC) was found in 61.2% of patients and 82.5% of controls, homozygous genotype (TT) in 6.3% of patients, and 2.5% of controls while heterozygous (CT) genotype in 32.5% of patients and 15% of controls (P = 0.011). Grade 1 PDUS was found in 30.6% of CC and 11.5% of CT and not found in TT genotypes. The grade 2 was found in 69.4%, 65.4%, and only 20% of CC, CT, and TT genotypes, respectively. The grade 3 was found in 80% of TT, 23.1% of CT, and none of CC genotypes (P < 0.001).

Conclusion
An association between VEGF gene SNP rs3025039 and increased risk for RA among a sample of Egyptian population was noticed. VEGF gene polymorphism appears to be a potential diagnostic activity indicator and a promising therapeutic target for RA patients.
Person-centred care in psychiatry: a clinical and philosophically informed approach
Authors: Glas Gerrit 
Published: December 2021

SUMMARYAfter many years of mental healthcare reform there is still a lot of unease among patients about healthcare workers’ lack of attention to their daily needs and to the tensions and ambiguities that accompany their attempts to integrate their condition into their lives. Person-centred care is often presented as a solution, but the term refers to many differing approaches and needs further specification depending on the problem it aims to resolve. This article presents and discusses a clinical and philosophically informed approach that flexibly focuses on the person- and context-bound aspects of the patient's condition and on the co-regulatory role of the clinician in the patient's attempt to regulate their condition. This approach is a way of thinking, rather than yet another model. It will be shown how this approach can be integrated in the core curriculum of specialty (residency) training in psychiatry.
Higher Incidence of Metabolic Syndrome in Black Women With Polycystic Ovary Syndrome: A Longitudinal Study
Authors: Lee Iris , Vresilovic Julia , Irfan Maryam , Gallop Robert , Dokras Anuja 
Published: December 2021

Abstract

Context
Cross-sectional studies have identified an increased risk of metabolic syndrome (MetSyn) in women with polycystic ovary syndrome (PCOS), but longitudinal data are limited and primarily include White and European cohorts.

Objective
To compare the longitudinal risk of MetSyn in Black and White women with PCOS and to identify potential factors mediating the risk of MetSyn.

Methods
Longitudinal cohort study with a follow-up of 5.3 years at an academic medical center

of
247 adult women with hyperandrogenic PCOS phenotype with 2 or more visits at least 3 years apart. The main outcome measure was incidence of MetSyn in Black and White women with PCOS.

Results
Using a mixed-effects model over time, the incidence of MetSyn was higher in Black women (45.9 ± 4.74 per 100 person-years) than in White women (31.3 ± 3.03 per 100 person-years) (P &lt; .01) after adjusting for age and medication status. This difference persisted among women under age 30. Among Black women who did not have MetSyn at their prior visit, 28.0% had MetSyn at the next visit, compared with 12.1% of White women after adjusting for age and medication status (P &lt; .01). In both races, the model-based estimated rates of MetSyn increased significantly with increase in body mass index and free testosterone.

Conclusion
We describe a persistent higher incidence of MetSyn in Black than in White women with PCOS. In addition to early cardiometabolic screening at the time of diagnosis, our findings highlight the need for ongoing and frequent screening in this population.
Analysis of the H-Ras mobility pattern in vivo shows cellular heterogeneity inside epidermal tissue
Authors: Gora Radoslaw J. , de Jong Babette , van Hage Patrick , Rhiemus Mary Ann , van Steenis Fjodor , van Noort John , Schmidt Thomas , Schaaf Marcel J. M. 
Published: December 2021

ABSTRACT
Developments in single-molecule microscopy (SMM) have enabled imaging individual proteins in biological systems, focusing on the analysis of protein mobility patterns inside cultured cells. In the present study, SMM was applied in vivo, using the zebrafish embryo model. We studied dynamics of the membrane protein H-Ras, its membrane-anchoring domain, C10H-Ras, and mutants, using total internal reflection fluorescence microscopy. Our results consistently confirm the presence of fast- and slow-diffusing subpopulations of molecules, which confine to microdomains within the plasma membrane. The active mutant H-RasV12 exhibits higher diffusion rates and is confined to larger domains than the wild-type H-Ras and its inactive mutant H-RasN17. Subsequently, we demonstrate that the structure and composition of the plasma membrane have an imperative role in modulating H-Ras mobility patterns. Ultimately, we establish that differences between cells within the same embryo largely contribute to the overall data variability. Our findings agree with a model in which the cell architecture and the protein activation state determine protein mobility, underlining the importance of SMM imaging for studying factors influencing protein dynamics in an intact living organism.
This article has an associated First Person interview with the first author of the paper.
Cognitive Universal Educational Actions of Primary Schoolchildren: Characteristics, Methods and Techniques of Formation
Authors: Romaeva N. , Astrecova N. 
Published: December 2021

The article discusses the actual problem of the formation of cognitive universal educational actions in younger schoolchildren in the context of
the implementation of the changing requirements of the Federal State Educational Standard of Primary General Education. The approaches
to the definition of concepts, groups and main characteristics of cognitive universal educational actions that students must master at the first
stage of school education are analyzed. The effective methods and techniques for the formation of this type of educational actions in younger
schoolchildren are presented.
The epidemiological characteristics of dengue in high-risk areas of China, 2013–2016
Authors: Sang Shaowei , Liu Qiyong , Guo Xiaofang , Wu De , Ke Changwen , Liu-Helmersson Jing , Jiang Jinyong , Weng Yuwei , Wang Yiguan 
Published: December 2021

Introduction
Dengue has become a more serious human health concern in China, with increased incidence and expanded outbreak regions. The knowledge of the cross-sectional and longitudinal epidemiological characteristics and the evolutionary dynamics of dengue in high-risk areas of China is limited.

Methods
Records of dengue cases from 2013 to 2016 were obtained from the China Notifiable Disease Surveillance System. Full envelope gene sequences of dengue viruses detected from the high-risk areas of China were collected. Maximum Likelihood tree and haplotype network analyses were conducted to explore the phylogenetic relationship of viruses from high-risk areas of China.

Results
A total of 56,520 cases was reported in China from 2013 to 2016. During this time, Yunnan, Guangdong and Fujian provinces were the high-risk areas. Imported cases occurred almost year-round, and were mainly introduced from Southeast Asia. The first indigenous case usually occurred in June to August, and the last one occurred before December in Yunnan and Fujian provinces but in December in Guangdong Province. Seven genotypes of DENV 1–3 were detected in the high-risk areas, with DENV 1-I the main genotype and DENV 2-Cosmopolitan the secondary one. The Maximum Likelihood trees show that almost all the indigenous viruses separated into different clusters. DENV 1-I viruses were found to be clustered in Guangdong Province, but not in Fujian and Yunnan, from 2013 to 2015. The ancestors of the Guangdong viruses in the cluster in 2013 and 2014 were most closely related to strains from Thailand or Singapore, and the Guangdong virus in 2015 was most closely related to the Guangdong virus of 2014. Based on closest phylogenetic relationships, viruses from Myanmar possibly initiated further indigenous cases in Yunnan, those from Indonesia in Fujian, while viruses from Thailand, Malaysia, Singapore and Indonesia were predominant in Guangdong Province.

Conclusions
Dengue is still an imported disease in China, although some genotypes continued to circulate in successive years. Viral phylogenies based on the envelope gene suggested periodic introductions of dengue strains into China, primarily from Southeast Asia, with occasional sustained, multi-year transmission in some regions of China.