bluuebunny/crossref_metadata_2025_split huggingface dataset:
Review on physicochemical, medicinal and nutraceutical properties of poppy seeds: a potential functional food ingredient
Authors: Muhammad Aleem , Akhtar Aqsa , Aslam Sadia , Khan Rao Sanaullah , Ahmed Zaheer , Khalid Nauman 
Published: October 2021

The poppy seed plant (Papaver somniferum L.) is well known for its health benefits. It contains plenty of nutrients including proteins, oil content, dietary fiber, antioxidants, tocopherols and other micronutrients. It is also under exploration considering its in vitro bioactive potential against various health issues such as hypertension, pain and cancer. Poppy is mainly grown for its opium and oil contents; however, poppy seeds are also widely used for cooking purposes. Poppy seeds contain various essential bioactive compounds including alkaloids, flavonoids, phenolic compounds and polyunsaturated fatty acids that can be effectively used as food ingredients in various applications. Poppyseed oil is considered a good quality oil owing to its rich polyunsaturated fatty acid content. Thus, tremendous potential exists in poppy seeds to be used as functional food ingredients and nutraceutical agents in various formulations. Medicinal and functional uses of poppy seed have been discussed, including some aspects of the utilization of the poppy seeds in the food industry. Further research is required to bring out its ingredient potential in food and health supplements. Keywords: Poppy, seed oil, health attributes, industrial uses, medicinal uses
Designing Cities Through Sound
Authors: Martucci Clara 
Published: October 2021

Sound shapes space. However, the architectural training of designers usually prioritizes visual aspects of a building or urban space without considering the sonic environment and auditory responses of humans who may engage or occupy the built environment. The concept of the “soundscape” brings together the visual and sonic environments, allowing designers to develop more nuanced, responsive, and effective spaces (Southworth, 1967, pp. 6-8)
Acousticians define soundscape as “a person’s perceptual construct of the acoustic environment of that place” (Kang & Schulte-Fortkamp, 2017, p. 5). People’s interpretation of auditory sensations can lead to either positive or negative feelings regarding that specific place. Because urban spaces include both a great number of sound sources and a high number of people occupying and moving through them, the sonic environments and urban soundscapes are complex, layered, and dense.
This research evaluates the sonic qualities of urban spaces to provide designers with a means by which these complex environments can be better understood, analyzed, and created. It draws on an expanding body of research in architectural acoustics, and direct observation of cities in the United States and Italy conducted during the COVID-19 pandemic. Rather than relying solely on numeric calculations, this work probes the notion of the “perceptual construct,” seeking to make visual these constructs. Drawings and photographs from different cities are used to study the form of the city through urban edges and the emerging concept of green acoustics. The work provides a way of creating a new architecture of public space through the lens of the sonic environment.
Evaluation of the antioxidant activity from bovine serum albumin protein fractions
Authors: León-Espinosa E.B. , Calderón-Domínguez G. , García-Garibay M. , Díaz-Ramírez M. , Cruz-Monterrosa R.G. , Ruiz-Hernández R. , Pérez-Ruiz R.V. , Jiménez-Guzmán J. 
Published: October 2021

Objective: Evaluate the antioxidant activity of protein fractions obtained from (bovine serum albumin) BSA protein hydrolysates.
Design / methodology / approach: Bioinformatics tools, such as the NCBI database, were used to search for primary sequences of BSA proteins. The methodology included a prediction of peptides with antioxidant activity through various bioinformatics servers. The antioxidant activity was determined by different methods. Statistical analysis was performed to evaluate possible significant differences using the Student Newman Keulls test for group comparison.
Results: Through in silica hydrolysis the following peptides were found: valine-alanine-phenylalanine (VAF), lysine-tryptophan (KW), phenylalanine-tyrosine (FY), alanine-proline (AP), among others that may have antioxidant activity. The results showed that the fraction <1 kDa hydrolyzed with chymotrypsin, this fraction showed 84% copper chelation, 61% iron chelation, while 75% inhibition of the DPPH radical. In the case of the fraction <1 kDa hydrolyzed with pepsin, it only showed 16% iron chelation, while in the other methods no value was detected.
Study limitations / implications: The enzyme used for enzymatic hydrolysis generates low degrees of hydrolysis and generates oligopeptide dipeptides that may not be as like some of the tested methods, in addition to the protein concentration in the fraction <1 kDa with pepsin it had very low values that could not be detected by some antioxidant methods.
Findings / conclusions: The antioxidant activity of the <1 kDa fraction obtained with chymotrypsin showed greater antioxidant and chelating activity, compared to the <1 kDa fraction obtained with pepsin. However, at the concentration of 2% and 5% fluctuations are observed in both fractions, because probably the composition of amino acids that is present in both fractions determines the activity in each of the tested methods
Analisa Hasil Pengelompokan Wilayah Kejadian Non-Kebakaran Menggunakan Agglomerative Hierachical Clustering di Semarang
Authors: Exasanti Desy , Jananto Arief 
Published: October 2021

Abstrak−Klasterisasi merupakan metode pengelompokan dari data yang sudah diketahui label kelasnya untuk menemukan klaster baru dari hasil observasi. Dalam klasterisasi banyak metode yaitu metode terpusat, hirarki, kepadatan dan berbasis kisi, namun dalam penelitian yang dilakukan ini dipilih metode berbasis hirarki. Metode hirarki ini bekerja melakukan pengelompokan objek dengan membentuk hirarki klaster namun bukan berarti selalu digambarkan dengan hirarki dalam organsasi. Dipilihnya Agglomerative Hierarchical Clustering dimana merupakan jenis dari bawah ke atas atau biasa disebut (bottom-up) dalam metode ini objek yang akan diuji dianggap sebagai objek tunggal sebagai klaster dan lalu dilakukan iterasi untuk menemukan klaster-klaster yang lebih besar. Data yang akan digunakan adalah data non-kebakaran pada Dinas Pemadam Kebakaran Kota Semarang ynng mana akan dilakukan pengelompokan wilayah penanganan non-kebakaran. Dinas Pemadam Kebakaran melakukan penanganan bukan hanya kebakaran saja namun ada banyak hal yang sebenarnya dapat ditangani oleh petugas pemadam kebakaran, kejadian non-kebakaran ada beberapa seperti evakuasi reptil, evakuasi kucing, penyelamatan korban kecelakaan dan lain sebagainya. Dari data non-kebakaran dari 16 kecamatan di Kota Semarang pada tahun 2019 akan dilakukan uji menggunakan tiga algoritma yaitu Single Lingkage, Average Linkage dan Complete Linkage . Adapun dari algoritma Single Linkage dilakukan prosedur pemusatan dari jarak terkecil antar objek data, algoritma Average Linkage dilakukan prosedur dari jarak rata-rata objek data, sedangkan jika algoritma Complete Linkage dilakukan prosedur pemusatan dari jarak yang terbesar. Implementasi dan visualiasi dari data uji coba yang dilakukan di penilitian ini menggunakan tools WEKA 3.8.4, Wakaito Environment Analysis for Knowledge atau yang biasa dikenal dengan WEKA ini merupakan software yang menggunakan bahasa pemrograman java. Dari dataset 380 data diambil sampel 100 data untuk diuji mengunakan WEKA menggunakan metode perhtungan jarak Manhattan Distance dengan 3 cluster. Hasil dari data uji coba dapat divisualisasikan dengan visualisasi dendogram pada fitur visualize tree dan jika dilakukan visualisasi dalam bentuk grafik dapat dilakukan menggunakan fitur visualize clusters assignment.
Teacher’s Strategies In Teaching Speaking During Covid-19 Pandemic
Authors: Fitri Arya , Hermansyah Hermansyah , Pratiwi Etty , Aswadijaya Aswadijaya 
Published: October 2021

The Covid-19 Pandemic situation made the Government announce online learning activities. This policy and it was relationship with teacher strategy at interesting teaching to learn. So that, This research was focused on the strategy that the teacher and used in impelemanting learning during the Covid-19 pandemic. Namely distance learning (PJJ), so that it could runned effectively, optimally, and run well. This research aimed to describe the strategy of teachers in teaching Speking during the Covid-19 Pandemic. This research used a descriptive qualitative approach. The research subject was class VIIB English teacher, and grade VIIB students. Data collection techniques in this research were observation, interviews, and documentation. The results of the research show that the teacher used an expository strategy that emphasized the teaching and learning process directed to achieve learning objectives. Analysis of online teacher learning methods with virtual media as a supporting means used two learning methods, first, the assignment method through the google classroom application, the results of the answer sheets were collected directly to the school, and then the second learning method was question and answer through Zoom Meeting and WhatsApp Group. The conclusion obtained from this research was that the teacher strategy in teaching speaking at Junior High School Prabumulih during the Covid-19 Pandemic could be seen from: teaching objectives, teaching approach, and teaching methods.
Students' Pronunciation Errors in English Silent Letters
Authors: Pusfarani Winda , Mukhrizal Mukhrizal , Puspita Hilda 
Published: October 2021

The objective of this research was to find out the types of pronunciation errors are made by the seventh semester students of English education study program at Bengkulu University in the academic year 2020/2021. This research used a descriptive quantitative method. 94 students as the total population 30 students were chosen randomly as the samples. The instrument of the research were pronunciation test and interview. The test comprises 52 sentences which contain 13 silent letters i.e. “k, gh, th, g, p, l, t, s, w, n, b, h, d”. Each letter appears 4 times in the test. The results of the research showed that the students made two types of errors namely pre-systematic errors and systematic errors. The pre-systematic errors occurred more frequently than the systematic errors. The highest errors made by the students were silent letters “g” (e.g. gnaw) and “b” (e.g. doubt) which total numbers of errors of each of them were 93 times (77%). It could be concluded that the students faced high difficulties in pronouncing the silent letters which completely different from Indonesian phonetic system. It was recommended that the English lecturers should pay more attention to students’ pronunciation and find more suitable method or technique of teaching it and students should study harder about the silent letters in English language.
The Clinicopathologic and Prognostic Significance of c-Myc Expression in Hepatocellular Carcinoma: A Meta-Analysis
Authors: Min Zhao , Xunlei Zhang , Haizhen Chen , Wenjing Zhao , Haiyan Yu , Xiaoyun Lu , Jianyun Zhou , Xudong Chen , Aiguo Shen 
Published: October 2021

Background: The incidence and mortality rates of hepatocellular carcinoma (HCC) are increasing worldwide. Therefore, there is an urgent need to elucidate the molecular drivers of HCC for potential early diagnosis and individualized treatment. Whether c-Myc expression plays a role in the clinicopathology and prognosis of patients with HCC remains controversial. This meta-analysis aimed to survey the prognostic role of c-Myc in HCC.Methods: We searched PubMed, Cochrane Library, Embase, Web of Science, and Google Scholar databases for studies published through March 2020 that examined the association between c-Myc expression and clinicopathology or prognosis in HCC patients. The pooled hazard ratios (HRs) and 95% confidence intervals (CIs) were used to investigate the prognostic significance of c-Myc expression. Odds ratios were calculated to evaluate the association between c-Myc expression and clinicopathologic features. We also tested for publication bias.Results: Our meta-analysis included nine studies with 981 patients with HCC published between 1999 and 2016. A meta-analysis of these studies demonstrated that high c-Myc expression indicated a poor overall survival (OS) (HR = 2.260, 95% CI: 1.660–3.080, and p &lt; 0.001) and disease-free survival (DFS) (HR = 1.770, 95% CI: 1.430–2.450, and p &lt; 0.001) in patients with HCC. However, high c-Myc expression was not associated with HBsAg, pathological type, TNM stage, or cirrhosis. We did not find any significant publication bias among the included studies, indicating that our estimates were robust and reliable.Conclusion: c-Myc overexpression could predict poor OS and DFS in HCC patients. c-Myc could be a useful prognostic biomarker and therapeutic target for HCC.
Surgical Outcome Prediction Using a Four-Dimensional Planning Simulation System With Finite Element Analysis Incorporating Pre-bent Rods in Adolescent Idiopathic Scoliosis: Simulation for Spatiotemporal Anatomical Correction Technique
Authors: Tachi Hiroyuki , Kato Koji , Abe Yuichiro , Kokabu Terufumi , Yamada Katsuhisa , Iwasaki Norimasa , Sudo Hideki 
Published: October 2021

An optimal surgical strategy for adolescent idiopathic scoliosis (AIS) is to provide maximal deformity correction while preserving spinal mobile segments as much as possible and obtaining a balanced posture. From a spatiotemporal deformity correction standpoint, we recently showed that anatomical four-dimensional (4D) spinal correction could be accomplished by curving the rod. In the surgical procedure, two rods are bent identically to confirm spinal anatomical alignment without referring to the intraoperative alignment of the deformity. Therefore, anatomically designed rods have been developed as notch-free, pre-bent rods for easier anatomical reconstruction. In addition to providing the best spinal instrumentation configurations as pre-bent rods, prediction of surgical outcome along with its biomechanical impact can be obtained by simulation of the surgical procedures with computer modeling. However, an objective model that can simulate the surgical outcome in patients with AIS has not been completely elucidated. The present study aimed to compare simulated deformity corrections based on our newly developed spatiotemporal morphological 4D planning simulation system incorporating pre-bent rods and actual deformity corrections in patients with AIS. A consecutive series of 47 patients who underwent anatomical posterior correction for AIS curves were prospectively evaluated. After multilevel facetectomy, except for the lowest instrumented segment, 11 types of pre-bent rods were used. Patient demographic data, radiographic measurements, and sagittal rod angles were analyzed within 1 week of surgery. Our simulation system incorporating pre-bent rods showed a significant correlation with the actual postoperative spinal alignment. The present study demonstrated the feasibility of our simulation system and the ability to simulate the surgical procedure using the pre-bent rods. The simulation system can be used to minimize the differences between the optimal and possible outcomes related to the instrumentation levels and rod shapes. Preoperative assumption of rod shape and length can contribute to a reduction in operative time which decreases blood loss and risk of infection. The results of the finite element analysis in the simulation system measured for each individual patient would also provide a more realistic representation of the surgical procedures.
Dapagliflozin, as Add-on Therapy in Type 2 Diabetes Patients, Is Associated With a Reduction in Albuminuria and Serum Transaminase Levels
Authors: Benjamin Silas , Ramanjaneya Manjunath , Butler Alexandra E. , Janjua Imran , Paramba Firjeeth , Palaki Jafer , Kubaisi Aisha Al , Chandra Prem , Abdalhakam Ibrahem , Massodi Nasseer Ahmad 
Published: October 2021

IntroductionSGLT-2 inhibitors are shown to be nephroprotective, slowing progression of nonalcoholic steatohepatitis (NASH) in addition to improving glycemic control in patients with type 2 diabetes (T2D). To date, no real-life clinical data is available on the effect of SGLT-2 inhibitors on urine albumin-creatinine ratio (ACR) and liver enzymes in a Middle Eastern population. Therefore, we evaluated the effect of dapagliflozin (DAPA) on urine ACR, alanine aminotransferase (ALT) and aspartate aminotransferase (AST) when added to standard therapy for T2D.MethodsThis is an observational study of 40 patients with T2D in whom DAPA was added to their existing anti-diabetic regimen to improve glycemic control. The primary outcomes were changes in serum transaminase level and urine albumin-to-creatinine ratio (ACR). Secondary outcomes include changes in glycosylated hemoglobin (HbA1C), body mass index (BMI), oral hypoglycemic agents and insulin dose.ResultsWhole group analysis showed a reduction in ALT (p&lt;0.0001), (AST) (p=0.009), ACR (p=0.009) and BMI (p&lt;0.0001) following DAPA treatment. Further sub-group analysis showed that patients on insulin and DAPA combination had a reduction in ACR (p=0.0090), ALT (p=0.0312), BMI (p=0.0007) and HbA1c (p&lt;0.0001) compared to the sulfonylurea and DAPA combination group. In the sulfonylurea and DAPA combination group, there was a reduction in the sulfonylurea requirement following DAPA therapy (p=0.0116), with reductions in ALT (p=0.0122), AST (p=0.0362), BMI (p=0.0026) and HbA1c (p&lt;0.0001) but with no change in ACR (p=0.814).ConclusionIn routine clinical practice, the addition of DAPA to standard medical therapy is well tolerated and beneficial for T2D patients and is associated with a reduction of ALT and ACR.
Identification of Key eRNAs for Spinal Cord Injury by Integrated Multinomial Bioinformatics Analysis
Authors: Huang Runzhi , Wang Siqiao , Zhu Rui , Xian Shuyuan , Huang Zongqiang , Cheng Liming , Zhang Jie 
Published: October 2021

Background: Spinal cord injury (SCI) is a severe neurological deficit affecting both young and older people worldwide. The potential role of key enhancer RNAs (eRNAs) in SCI remains elusive, which is a prominent challenge in the trauma repair process. This study aims to investigate the roles of key eRNAs, transcription factors (TFs), signaling pathways, and small-molecule inhibitors in SCI using multi-omics bioinformatics analysis.Methods: Microarray data of peripheral blood mononuclear cell (PBMC) samples from 27 healthy volunteers and 25 chronic-phase SCI patients were retrieved from the Gene Expression Omnibus database. Differentially expressed transcription factors (DETFs), differentially expressed enhancer RNAs (DEeRNAs), and differentially expressed target genes (DETGs) were identified using the Linear Models for Microarray Data (limma) package. Fraction of immune cells was estimated using CIBERSORT algorithm. Gene Set Variation Analysis (GSVA) was applied to identify the downstream signaling pathways. The eRNA regulatory network was constructed based on the correlation results. Connectivity Map (CMap) database was used to find potential drugs for SCI patients. The cellular communication analysis was performed to explore the molecular regulation mechanism of SCI based on single-cell RNA sequencing (scRNA-seq) data. Chromatin immunoprecipitation sequencing (ChIP-seq) and Assay for Transposase-Accessible Chromatin using sequencing (ATAC-seq) data were used to validate the key regulatory mechanisms. scRNA-seq dataset was used to validate the cell subtype localization of the key eRNAs.Results: In total, 21 DETFs, 24 DEeRNAs, and 829 DETGs were identified. A regulatory network of 13 DETFs, six DEeRNAs, seven DETGs, two hallmark pathways, two immune cells, and six immune pathways was constructed. The link of Splicing factor proline and glutamine rich (SFPQ) (TF) and vesicular overexpressed in cancer prosurvival protein 1 (VOPP1) (eRNA) (R = 0.990, p &lt; 0.001, positive), VOPP1 (eRNA) and epidermal growth factor receptor (EGFR) (target gene) (R = 0.974, p &lt; 0.001, positive), VOPP1, and T helper (Th) cells (R = −0.987, p &lt; 0.001, negative), and VOPP1 and hallmark coagulation (R = 0.937, p &lt; 0.001, positive) was selected. Trichostatin A was considered the best compound target to SCI-related eRNAs (specificity = 0.471, p &lt; 0.001).Conclusion: VOPP1, upregulated by SFPQ, strengthened the transient expression of EGFR. Th cells and coagulation were the potential downstream pathways of VOPP1. This regulatory network and potential inhibitors provide novel diagnostic biomarkers and therapeutic targets for SCI.
Evaluation of Endothelial Dysfunction and Inflammatory Vasculopathy After SARS-CoV-2 Infection—A Cross-Sectional Study
Authors: Jud Philipp , Gressenberger Paul , Muster Viktoria , Avian Alexander , Meinitzer Andreas , Strohmaier Heimo , Sourij Harald , Raggam Reinhard B. , Stradner Martin Helmut , Demel Ulrike , Kessler Harald H. , Eller Kathrin , Brodmann Marianne 
Published: October 2021

Background: Rising data suggest that COVID-19 affects vascular endothelium while the underlying mechanisms promoting COVID-19-associated endothelial dysfunction and inflammatory vasculopathy are largely unknown. The aim was to evaluate the contribution of COVID-19 to persisting vascular injury and to identify parameters linked to COVID-19-associated endothelial dysfunction and inflammatory vasculopathy.Methods: In a cross-sectional design, flow-mediated dilation (FMD), nitroglycerine-related dilation (NMD), pulse-wave velocity (PWV), augmentation index, intima-media thickness (IMT), compounds of the arginine and kynurenine metabolism, homocysteine, von Willebrand factor (vWF), endothelial microparticles (EMP), antiendothelial cell antibodies, inflammatory, and immunological parameters, as well as nailfold capillary morphology were measured in post-COVID-19 patients, patients with atherosclerotic cardiovascular diseases (ASCVD) and healthy controls without prior or recent SARS-CoV-2 infection.Results: Post-COVID-19 patients had higher values of PWV, augmentation index, IMT, asymmetric and symmetric dimethylarginine, vWF, homocysteine, CD31+/CD42b– EMP, C-reactive protein, erythrocyte sedimentation rate, interleukin-6, and β-2-glycoprotein antibodies as well as lower levels of homoarginine and tryptophan compared to healthy controls (all with p &lt; 0.05). A higher total number of pathologically altered inflammatory conditions and higher rates of capillary ramifications, loss, caliber variability, elongations and bushy capillaries with an overall higher microangiopathy evolution score were also observed in post-COVID-19 patients (all with p &lt; 0.05). Most parameters of endothelial dysfunction and inflammation were comparably altered in post-COVID-19 patients and patients with ASCVD, including FMD and NMD.Conclusion: COVID-19 may affect arterial stiffness, capillary morphology, EMP and selected parameters of arginine, kynurenine and homocysteine metabolism as well as of inflammation contributing to COVID-19-associated endothelial dysfunction and inflammatory vasculopathy.