bluuebunny/crossref_metadata_2025_split huggingface dataset:
Coal Fly Ash liquid on Growth amendments of Eri silkworm and amelioration of horticulture crops
Authors: Sahayaraj Kitherian , Arumugavel Suppiah , Ponsankar Athirstam , Madasamy Mariappan 
Published: January 2021

Abstract
To enhanced the development and reproduction of Eri silkworm incorporation of Coal Flyash Liquid (CFL) in their natural diet was developed to evaluate the growth and fecundity rate of eri silkworm. energy dispersive analysis of castor leaves treated with CFL at 10% concentration revealed the presence of five major minerals viz., magnesium (Mg), silica (Si), chlorine (Cl), potassium (K) and calcium (Ca). While CFL incorporated castor leaf enhanced the pupation rate, shell weight, cocoon shell ratio, fecundity and egg hatchability of the silkworm. In contrast, pupal weight was increased at lower concentration of CFL with a weight of 2.39 mg. A significant difference was observed in rearing performance of eri silkworm at different concentrations of CFL incorporated castor leaf. Relative growth rate of the eri silkworm was increased to 84% when larvae treated with CFL at 5% concentration. Further, CFL at the concentration of 5% enhanced the seed germination percentage and at the higher concentration of 10% increased the root-shoot ratio, relative water content and vigour index of cucumber. Similar response of increase in studied growth parameters was also observed in radish seedlings when treated with CFL. The physiochemical properties of CFL amended soil (5%) showed that available phosphorous level and potassium level was increased respectively to 9.52% and 53.92% in comparison to control. It could be concluded that minerals present in CFL may be influential and responsible for the growth of eri silkworm and seedling of cucumber and radish.
Exploring magmatic gas and subvolcanic hydrothermal system interactions: Mineralogy and sulfur isotope characteristics of the Holocene volcanic products at Mt. Tangkuban Parahu, Indonesia.
Authors: Angkasa Syahreza Saidina , Tsukasa Ohba , Takumi Imura , Manalo Pearlyn , Ryohei Takahashi 
Published: January 2021

Abstract
A subvolcanic-hydrothermal system involves complex interaction between magma, magmatic fluids, and hydrothermal system at stratovolcanoes in subduction setting. These interactions are responsible for magmatic-hydrothermal eruption associated with rapid injection of magmatic gas into hydrothermal system at a certain depth of volcanic edifice. However, capturing these interactions is challenging due to inaccessibility to the crater conduit within the edifice. Therefore, we selected a method to analyze the volcanic products from several episodic phreatic and phreatomagmatic eruptions during the Holocene at Tangkuban Parahu, Indonesia. In this context, Holocene volcanic products are one of the best examples to understand an interplay between magma, magmatic fluids, and hydrothermal system in producing violent eruptions. In this study, we carried out petrological and sulfur isotope analysis only for the hydrothermally altered lithic ash particles, a part of proximal volcanic products. Mineral assemblages mostly exhibit a typical acid-sulfate and advanced argillic alteration, consisting of alunite, kaolinite, and silica minerals. Acid-sulfate and advanced argillic alteration indicates that those mineral assemblages were formed under the formation temperature ranging from ~100 to ~260 . The calculated temperature from sulfur isotopic fractionation of sulfate-sulfide shows 230-240 , which is almost identical with assigned temperature from mineral assemblages. Sulfur isotope and jarosite occurrence indicate the supergene alteration associated with oxygen entrainment to the hydrothermal system that oxidize pyrite to jarosite. Sulfur isotopic variation throughout the studied stratigraphy represents influx of magmatic gas to the hydrothermal system. Moreover, zoned P-bearing alunite represents repetitive injection of magmatic gas to the active acidic hydrothermal system, which also indicates the magmatic-hydrothermal interaction below the crater. Occurrence of enargite and chalcopyrite represents the nature of upper-level high sulfidation system at shallow volcanic edifice of the Tangkuban Parahu volcano. Furthermore, we showed that coupled petrological and sulfur isotope analysis has paramount importance to evaluate the conditions of the subvolcanic hydrothermal system, magmatic-hydrothermal interaction, and the origin of steam-blast eruptions at volcanoes that contain subvolcanic-hydrothermal systems.
The Wear Rate and Survivorship in Total Hip Arthroplasty using a Third- Generation Ceramic Head on a Conventional Polyethylene liner: A Minimum of 15-year Follow-up
Authors: Shim Bum-Jin , Choi Chang Hyun , Woo Inha , Park Chan Ho 
Published: January 2021

Abstract
Background: Recently, the use of ceramic-on-polyethylene (CoP) bearing surfaces has increased in the United States with the development of material properties. However, it remains controversial which bearing couples are effective. The purpose of this study was to evaluate the wear and survival rates of third-generation ceramic heads on a conventional polyethylene liner. Methods: We retrospectively reviewed 160 hips (147 patients with a mean age of 56 years) who underwent total hip arthroplasty using the third-generation ceramic head on a conventional polyethylene liner from March 1998 to August 2003. The wear rate was evaluated using the PolyWare program Version 8 (Draftware Developers Inc., IN, USA) in 32 hips followed-up for at least 15 years. In addition, we evaluated the Kaplan-Meier survivorship.Results: Linear wear and volumetric wear rates were 0.102±0.57 mm/year and 30.2±27.5 mm3/year, respectively. Fourteen reoperations due to all causes and 10 revisions were performed during the follow-up period. The 15-year survival rate was 84.1% for any surgery as the endpoint and 87.6% for the revision surgery as the endpoint. Most of the causes of revision were cup loosening, and no ceramic head fracture occurred.Conclusions: The CoP bearing surface maintains the advantages of the soft polyethylene surface and has good resistance to wear. Therefore, alumina ceramic on newer polyethylene could certainly be a good alternative bearing couple without any concern for ceramic fractures, especially in younger patients.Level of evidence: Level III, retrospective cohort design
Development of Foot Length in Children with Congenital Clubfoot up to 7 Years of Age: A Prospective Follow-Up Study
Authors: Manousaki Evgenia , Esbjörnsson Anna-Clara , Hägglund Gunnar , Andriesse Hanneke 
Published: January 2021

Abstract
Background: Clubfeet are typically shorter than normal feet. This study aimed to describe the development of foot length in a consecutive series of children with congenital clubfoot and to relate foot length to development of relapse and motion quality.Methods: Foot length was measured every 6 months in 72 consecutive children with congenital clubfoot (29 bilateral) aged from 2 to 7 years. The initial treatment was nonsurgical followed by standardized orthotic treatment. Foot length growth rate was calculated every half year. In children with unilateral clubfeet, the difference in foot length between the clubfoot and the contralateral foot was calculated. Motion quality was evaluated by the Clubfoot Assessment Protocol (CAP). Student’s t test, the Mann–Whitney U test and Spearman’s correlation were used for group comparisons. Bonferroni correction was used when multiple comparisons were performed.Results: Clubfeet were smaller (P < 0.001) than reference feet at all ages but had a similar growth rate up to age 7. Unilateral clubfeet with greater difference in size compared with the contralateral foot at the first measurement, relapsed more frequently (P = 0.016) and correlated with poorer motion quality (r = 0.4; P = 0.011).Conclusions: Clubfeet were smaller than reference feet at all ages but had a similar growth rate. Children with unilateral clubfeet and greater foot length difference at 2 years of age had a higher tendency to relapse and poorer motion quality at 7 years of age.
Surface Landmarks Combined With Image-guided Sinus Location in the Retrosigmoid Approach and Their Clinical Feature Analysis
Authors: Wu Weichi , Ke Chao , Liu Zhaoyang , Guo Xiaoyu , Zhou Yi , Lin Zhu , Liu Haibin , Mou Yonggao , Zhang Ji 
Published: January 2021

Abstract
Background During craniotomy for the cerebellopontine angle (CPA) lesion by the typical retrosigmoid approach, the exact exposure of the margin of the venous sinuses complex remains an essential but risky step. This study aimed to reveal the exact position of asterion and sinuses by combining preoperative imaging with intraoperative landmarks. and analyse their clinical features. Methods From February 2008 through November 2019, 94 patients who underwent removal of vestibular schwannoma (VS) through retrosigmoid craniotomies were enrolled in the series. We utilized preoperative images, including computed tomography (CT) and/or magnetic resonance imaging (MRI) combined with intraoperative anatomical landmarks, to determine the exact location of the sigmoid sinus and the transverse and sigmoid sinuses junction (TSSJ). MRI T1 sequences with gadolinium and/or the CT bone window were used to measure the distance relationship of the asterion to the sigmoid sinus.Results In 94 cases of retrosigmoid craniotomies, we observed the asterion lay 12.71 millimeter on the posterior to the body surface projection of the TSSJ averagely. Intraoperative surface landmarks combined with preoperative image information identifying the distance from the asterion to sigmoid sinus at the transverse sinus level, enabled an appropriate initial burr-hole (the margin of the TSSJ ). Just one case had a minor laceration of the sigmoid sinus when the bone flap was opened.Conclusions By combining intraoperative anatomical landmarks with preoperative image information, the margin of the venous sinuses, especially the inferior margin of the transverse sinus in the retrosigmoid approach can be well and truly identified. The distance from the intersection of the asterion and occipitomastoid suture to the TSSJ is the shortest between the occipitomastoid suture and the sigmoid sinus.
LncRNA DLGAP1-AS2&nbsp;Suppresses the Maturation of miR-16 to Suppress Cell Invasion and Migration of Ovarian Cancer Cells
Authors: Luo Jie , Zhang Yuqiang , Zheng Ting , Jing Yongping , Cao Rongyu , Wu Minmin , Fan Die , Tao Ying , Zhao Mandan 
Published: January 2021

Abstract
Background: This study aimed to explore the role of lncRNA DLGAP1-AS2 in ovarian cancer (OC). Methods: Expression of DLGAP1-AS2, mature miR-16 and miR-16 precursor in paired OC tissues and non-tumor tissues collected from 62 OC patients was determined by RT-qPCR. Correlations were analyzed by Pearson’s correlation coefficient. Overexpression of DLGAP1-AS2 was achieved in OC cell line UWB1.289 to explore the effects of DLGAP1-AS2 overexpression on the expression of mature miR-16 and miR-16 precursor by RT-qPCR. Transwell assays were performed to analyze the role of DLGAP1-AS2 and miR-16 in regulating the invasion and migration of UWB1.289 cells.Results: DLGAP1-AS2 was upregulated in OC and inversely correlated with mature miR-16, but not miR-16 precursor. In OC cells, DLGAP1-AS2 overexpression resulted in downregulated mature miR-16, but failed to significantly affect the expression of miR-16 precursor. Cell invasion and migration analysis showed that DLGAP1-AS2 overexpression reduced the inhibitory effects of miR-16 overexpression on cell invasion and migration.Conclusions: DLGAP1-AS2 may suppress the maturation of miR-16 to suppress cell invasion and migration of OC cells.
Determining the Sex-Specific Distributions of Average Daily Alcohol Consumption Using a Machine Learning Method: Is There a Separate Distribution for People With Alcohol Dependence?
Authors: Jiang Huan , Lange Shannon , Tran Alexander , Imtiaz Sameer , Rehm Jürgen 
Published: January 2021

Abstract
BackgroundIt remains unclear whether alcohol use disorders (AUDs) can be characterized by specific levels of average daily alcohol consumption. The aim of the current study was to model the distributions of average daily alcohol consumption among those who consume alcohol and those with alcohol dependence, the most severe AUD, using various machine learning techniques. MethodsData from Wave 1 and Wave 2 of the National Epidemiologic Survey on Alcohol and Related Conditions were used in the current analyses. Clustering algorithms were applied in order to group a set of data points that represent the average daily amount of alcohol consumed. Gaussian Mixture Models (GMMs) were then used to estimate the likelihood of a data point belonging to one of the mixture distributions. Individuals were assigned to the clusters which had the highest posterior probabilities from the GMMs, and their treatment utilization rate was examined for each of the clusters. ResultsModeling alcohol consumption via machine learning technique was feasible. The clusters identified did not point to alcohol dependence as a separate cluster characterized by a higher level of alcohol consumption. Among both females and males with alcohol dependence, daily alcohol consumption was relatively low. ConclusionsOverall, we found little evidence for clusters of people with the same drinking distribution, which could be characterized as clinically relevant for people with alcohol use disorders as currently defined.
Neutral pH&nbsp;Formulation for 6-[18F]fluoro-ʟ-DOPA&nbsp;With High Radiochemical Stability
Authors: Lee Sang Ju , Oh Seung Jun 
Published: January 2021

Abstract
6-[18F]Fluoro-L-DOPA(FDOPA) has always been generally produced under strong acidic conditions, i.e. as an injectable solution of pH 2~3, due to its low stability at a higher or neutral pH. This necessitates the pre-treatment neutralization of this agent with an injectable NaHCO3 solution. We have developed a neutral pH formulation for [18F]FDOPA using ethanol (EtOH) and phosphate buffer to overcome the radioactive and enantiomeric stability problems at a higher pH. Upon [18F]FDOPA generation by nucleophilic substitution methods, we investigated its radiochemical and enantiomeric purity in accordance with the various pH after 6 hours. After EtOH and three kinds of buffer were added, we further examined this purity at pH 6 ~ 7 after 6 hours. The ascorbic acid did not stabilize the radiochemical purity at the higher pH. A 5% EtOH and PBS buffer matrix produced the best stability for radiochemical and enantiomeric purity at pH 6.5 at the 6 hour time point. This combination maintained a > 95% radiochemical and enantiomeric purity at 6 hours after EOS (end of synthesis). Our new formulation for [18F]FDOPA thus showed a high stability at neutral pH and satisfied QC requirements which was listed in European Pharmacopeia. It has also been approved by the Korean Ministry of Food and Drug Safety.
The Combination of 13N-ammonia and 11C-methionine in Differentiation of Residual/Recurrent Pituitary Adenoma From the Pituitary Gland Remnant After Trans-sphenoidal Adenomectomy
Authors: ding lei , He Qiao , Luo Ganhua , Long Yali , Li Ruocheng , Zhang Fangling , Zhang Xiangsong 
Published: January 2021

Abstract
ObjectiveTo assess the usefulness of 13N-ammonia and 11C- Methionine (MET) PET/CT in the differentiation of residual/recurrent pituitary adenoma (RPA) from the pituitary gland remnant (PGR) after trans-sphenoidal adenomectomy.Patients and MethodsBetween June 2012 to December 2019, 19 patients [clinical/MRI suggestion of RPA (14 female and 5 male, mean age: 44.86 ± 15.58 years, range: 18–79 years)] with a history of trans-sphenoidal adenomectomy before PET/CT scans in our department were enrolled in this study. The maximum standard uptake value (SUVmax) of the target and gray matter was measured, and then the T/G ratio was calculated. First, the T/G ratios of RPA and PGR for each tracer were compared by Student t-test. Second, the T/G ratios of the two tracers were adopted as multiple variables for canonical discrimination analysis. According to the canonical discriminant function, every patient was classified into one group. A P value less than 0.05 was considered statistically significant. ResultsThe T/G ratios of 13N-ammonia were significantly higher in PGR than RPA (1.58±0.69 vs 0.63±1.37, P< 0.001), whereas the T/G ratios of 11C-MET were significantly lower in PGR than RPA (0.78±0.35 vs 2.17±0.54, P < 0.001). By the canonical discriminant analysis, we calculated the predicted accuracy of RPA (100%), PGR (92.9%), and the overall predicted accuracy (96.43%). ConclusionsThe combination of 13N-ammonia and 11C-MET PET/CT is valuable in the differentiation of RPA from PGR after trans-sphenoidal adenomectomy.
Genomic Profiling of Small Superficial Non-Ampullary Duodenal Epithelial Tumors
Authors: Miyamoto Shuichi , Suda Goki , Ishikawa Marin , Hayashi Hideyuki , Nimura Satoshi , Matsuno Yoshihiro , Mori Ryo , Tanishima Shigeki , Kudo Takahiko , Takagi Tomofumi , Yamamoto Yoshiya , Ono Shoko , Shimizu Yuichi , Sakamoto Naoya 
Published: January 2021

Abstract
Introduction The mechanism underlying carcinogenesis and the genomic features of superficial non-ampullary duodenal epithelial tumors (SNADETs) have not been elucidated in detail. In this study, we examined the genomic features of incipient SNADETs, such as small lesions resected via endoscopic treatment, using next-generation sequencing (NGS). Methods Twenty consecutive patients who underwent endoscopic treatment for SNADETs of less than 20 mm between January 2017 and December 2017 were enrolled. Targeted genomic sequencing was performed through NGS using a 160 cancer-related gene panel. We examined the alteration/mutation frequencies in SNADETs. Results The maximum size of the SNADETs examined in this study was 12 mm in diameter. Five SNADETs were classified as low grade dysplasia (LGD) tumors, while 14 SNADETs were classified as high grade dysplasia tumors. Only one carcinoma-in-situ tumor was detected. We obtained NGS data for 16 samples. APC alterations were detected in 81% of samples (13/16). KRAS, BRAF, and TP53 alterations were detected in 25% (4/16), 18.8% (3/16), and 6.3% (1/16) of cases, respectively. Conclusions We detected APC alterations in most small SNADETs resected via endoscopic treatment, from LGD to carcinoma samples. Even in SNADETs classified as small LGD, KRAS and BRAF alterations were present in a few samples.
DenovoProfiling: A Webserver for De Novo Generated Molecule Library Profiling
Authors: Liu Zhihong , Du Jiewen , Liu Bingdong , Cui Zongbin , Fang Jiansong , Xie Liwei 
Published: January 2021

Abstract
With the advances of deep learning techniques, various architectures for molecular generation have been proposed for de novo drug design. Successful cases from academia and industrial demonstrated that the deep learning based de novo molecular design could efficiently accelerate the drug discovery process. The flourish of the de novo molecular generation methods and applications created great demand for the visualization and functional profiling for the de novo generated molecules. The rising of publicly available chemogenomic databases lays good foundations and create good opportunities for comprehensive profiling of the de novo library. In this paper, we present DenovoProfiling, a web server dedicated for de novo library visualization and functional profiling. Currently, DenovoProfiling contains six modules: (1) identification & visualization, (2) chemical space, (3) scaffold analysis, (4) molecular alignment, (5) target & pathways, and (6) drugs mapping. DenovoProfiling could provide structural identification, chemical space exploration, drugs mapping, and targets & pathways. The comprehensive annotated information could give user a clear picture of their de novo library and could provide guidance in the further selection of candidates for synthesis and biological confirmation. DenovoProfiling is freely available at http://denovoprofiling.xielab.net.
Cheese, Microbiome and Obesity: A Love Triangle
Authors: Meyer Jeremy , Roos Elin , Schrenzel Jacques , Ris Frédéric , Davies Justin 
Published: January 2021

Abstract
IntroductionRecent evidence has shown the importance of the microbiome in the development and maintenance of obesity. Probiotics were proposed for modulating the microbiome in order to achieve weight loss.MethodsData related to consumption of dairy food in European countries were obtained from the Global Dairy Consumption Report of the European Association of Dairy Trade Eucolait. Age-adjusted prevalence of obesity in European countries was extracted from the World Health Organization Global Health Repository. Linear regression was performed.ResultsConsumption of cheese in kilogram per capita was inversely correlated to the age-adjusted prevalence of obesity in 26 European countries (R2: 0.298, p=0.004).ConclusionConsumption of cheese appears to protect against weight gain. This finding deserves further confirmation by longitudinal studies or randomized controlled trials.
Are Topical Skin Adhesives an Option for Wound Closure Following Musculoskeletal Oncology Surgery? A STROCSS-compliant Observational Study
Authors: Chobpenthai Thanapon , Poosiripinyo Thanate , Tuntarattanapong Pakjai , Thanindratarn Pichaya , Trathitephun Warayos 
Published: January 2021

Abstract
Background:2-octyl cyanoacrylate (2OCA) is a high-viscosity medical-grade tissue adhesive used routinely. However, no studies have evaluated its use in musculoskeletal oncology surgery. Methods:We enrolled 99 patients undergoing musculoskeletal oncology surgery. 2OCA was chosen for wound closure, and it was applied by a specific surgeon for all patients. Drying times for the adhesive were recorded, and photographs were obtained intra-operatively. Post-treatment follow-up constituted queries regarding pain level, and recording incisional dehiscence, wound infection, hematoma, and incisional bleeding. Data collection was performed post-operatively at 48 hours, 5–10 days, 14 days, and 30 days. Other adverse events were documented. Results:2OCA was applied to 110 incisions in 99 patients constituting 62 female patients and 37 male patients. The mean age of patients was 50.41 (±16.83) years; mean incision length was 10.24 (±5.7) cm; and the mean pain score using a visual analogue scale (VAS) was 2.37 on post-operative day 7. The mean drying time was 1.81 (±0.59) minutes; 91 (91%) patients reported excellent and superior satisfaction, and the remaining patients reported “good” (6%) and “fair” (2%) satisfaction. The percentage of dehiscence, hematoma, and keloid formation was considerably low. Conclusion:2OCA was safe in musculoskeletal oncology surgical incisions in this study. The incidence of post-operative adverse events was low. However, some patients developed a hematoma. Post-operative pain was low, and patient satisfaction was high. 2OCA can be a practical alternative to traditional suture closure for skin incisions after musculoskeletal surgery.