bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mechanisms of sterile inflammation after intravitreal injection of antiangiogenic drugs: a narrative review
Authors: Anderson William J. , da Cruz Natasha Ferreira Santos , Lima Luiz Henrique , Emerson Geoffrey G. , Rodrigues Eduardo Büchele , Melo Gustavo Barreto 
Published: May 2021

AbstractBackgroundIntraocular inflammation is an uncommon but potentially vision-threatening adverse event related to anti-VEGF therapy. This is of increasing importance given both the volume of injections performed, as well as the increased prevalence of inflammation seen with newer anti-VEGF agents. Brolucizumab, the newest anti-VEGF agent, has been associated with an inflammatory retinal vasculitis and the underlying mechanism is unclear. Reviewing potential mechanisms and clinical differences of intraocular inflammation may assist clinicians and scientists in reducing the risk of these events in the future.ObservationsTwo types of inflammation are seen with intravitreal injections, acute onset sterile inflammation and delayed onset inflammatory vasculitis. Acute onset inflammation can be subcategorized into subclinical anterior chamber inflammation and sterile uveitis/endophthalmitis. Subclinical anterior chamber inflammation can occur at rates as high as 19% after intravitreal anti-VEGF injection. Rates of sterile uveitis/endophthalmitis range from 0.05% to 4.4% depending on the anti-VEGF agent. Inflammatory vasculitis is only associated with brolucizumab and occurred in 3.3% of injections according to the post hoc review of the HAWK/HARRIER data. In addition, silicone oil from syringes can induce immunogenic protein aggregates. Agitation of the syringe, freeze thawing, shipping and improper storage prior to injection may increase the amount of silicone oil released from the syringe.ConclusionThe main factors which play a role in intraocular inflammation after anti-VEGF injection can be divided into three causes: patient-specific, medication-specific and delivery-specific. The majority of clinically significant inflammation seen after intravitreal injection is an acute onset inflammatory response with most patients recovering baseline VA in 3–5 weeks. The presence of pain, hypopyon, severe anterior chamber reaction, hyperemia and significant vision loss may help distinguish infectious from non-infectious etiologies of post injection inflammation. Avoiding temperature fluctuation, mechanical shock, agitation during transport and handling of syringes/drugs, and the use of SO-free syringes may help minimize intraocular inflammation. While a definitive mechanism has not yet been established, current knowledge of the clinical presentation and vitreous histopathology of brolucizumab-retinal vasculitis favors an auto-immune type IV hypersensitivity reaction.
What do laboratory-forgetting paradigms tell us about use-inspired forgetting?
Authors: Scotti Paul S. , Maxcey Ashleigh M. 
Published: May 2021

AbstractDirected forgetting is a laboratory task in which subjects are told to remember some information and forget other information. In directed forgetting tasks, participants are able to exert intentional control over which information they retain in memory and which information they forget. Forgetting in this task appears to be mediated by intentional control of memory states in which executive control mechanisms suppress unwanted information. Recognition-induced forgetting is another laboratory task in which subjects forget information. Recognizing a target memory induces the forgetting of related items stored in memory. Rather than occurring due to volitional control, recognition-induced forgetting is an incidental by-product of activating items in memory. Here we asked whether intentional directed forgetting or unintentional recognition-induced forgetting is a more robust forgetting effect. While there was a correlation between forgetting effects when the same subjects did both tasks, the magnitude of recognition-induced forgetting was larger than the magnitude of directed forgetting. These results point to practical differences in forgetting outcomes between two commonly used laboratory-forgetting paradigms.
Eocene to Oligocene nannofossils stratigraphy and environmental conditions in Izeh Province, Zagros Basin, East Tethys
Authors: Senemari Saeedeh , Jalili Farah 
Published: May 2021

AbstractData obtained from the calcareous nannofossils, distributed in the upper part of the Pabdeh Formation (Priabonian–Rupelian) and the lower part of the Asmari Formation (Chattian) in the Bid-Zard section, were used to investigate the Eocene to Oligocene palaeoenvironmental conditions in the southwest of Izeh, southwestern Iran (eastern Tethys). The upper part of the Pabdeh Formation was composed of shale, thin-bedded pelagic limestone and dolostone, which is disconformably overlain by the Asmari Formation. For the first time, 29 species of calcareous nannofossils belonging to 13 genera were identified in the studied section. The calcareous nannofossils in the upper part of the Pabdeh Formation indicate theIsthmolithus recurvusZone/Sphenolithus pseudoradiansZone (combined zone),Ericsonia subdistichaZone,Helicosphaera reticulataZone andSphenolithus praedistentusZone, from the Priabonian to the Rupelian. TheSphenolithus ciperoensisZone of the Chattian was identified in the lower part of the Asmari Formation. Calcareous nannofossil stratigraphy across the upper Eocene–Oligocene interval also reveals a disconformity at the Rupelian/Chattian transition due to a bio-event. Shallowing of the basin and environmental changes in this part of the Tethyan domain could have led to the lithostratigraphic and biostratigraphic changes. In fact, during the late Eocene to late Oligocene, marine phytoplankton was sensitive to climate changes such as decreasing temperature, as well as possibly to a nutrient increase and changes in basin depth.
Dual RNAseq highlights the kinetics of skin microbiome and fish host responsiveness to bacterial infection
Authors: Le Luyer J. , Schull Q. , Auffret P. , Lopez P. , Crusot M. , Belliard C. , Basset C. , Carradec Q. , Poulain J. , Planes S. , Saulnier D. 
Published: May 2021

Abstract
Background
Tenacibaculum maritimum is a fish pathogen known for causing serious damage to a broad range of wild and farmed marine fish populations worldwide. The recently sequenced genome of T. maritimum strain NCIMB 2154T provided unprecedented information on the possible molecular mechanisms involved in the virulence of this species. However, little is known about the dynamic of infection in vivo, and information is lacking on both the intrinsic host response (gene expression) and its associated microbiota. Here, we applied complementary omic approaches, including dual RNAseq and 16S rRNA gene metabarcoding sequencing using Nanopore and short-read Illumina technologies to unravel the host–pathogen interplay in an experimental infection system using the tropical fish Platax orbicularis as model.

Results
We showed that the infection of the host is characterised by an enhancement of functions associated with antibiotic and glucans catabolism functions but a reduction of sulfate assimilation process in T. maritimum. The fish host concurrently displays a large panel of immune effectors, notably involving innate response and triggering acute inflammatory response. In addition, our results suggest that fish activate an adaptive immune response visible through the stimulation of T-helper cells, Th17, with congruent reduction of Th2 and T-regulatory cells. Fish were, however, largely sensitive to infection, and less than 25% survived after 96 hpi. These surviving fish showed no evidence of stress (cortisol levels) or significant difference in microbiome diversity compared with controls at the same sampling time. The presence of T. maritimum in resistant fish skin and the total absence of any skin lesions suggest that these fish did not escape contact with the pathogen, but rather that some mechanisms prevented pathogens entry. In resistant individuals, we detected up-regulation of specific immune-related genes differentiating resistant individuals from controls at 96 hpi, which suggests a possible genomic basis of resistance, although no genetic variation in coding regions was found.

Conclusion
Here we focus in detail on the interplay between common fish pathogens and host immune response during experimental infection. We further highlight key actors of defence response, pathogenicity and possible genomic bases of fish resistance to T. maritimum.
Production of whey protein hydrolyzates and its incorporation into milk
Authors: John Jenny Ann , Ghosh Bikash C. 
Published: May 2021

Abstract
Whey proteins provide an excellent source of low-molecular-weight bioactive peptides with important functional properties and bioactivities like antihypertensive, opioid, and antimicrobial effects. Presence of peptide molecules with lower molecular weight has a great role in food for health promotion. In this investigation, the release of low-molecular-weight peptides from whey protein concentrate was attempted by using enzymatic digestion. The hydrolyzate was then incorporated into milk to obtain enriched milk (EM) with low-molecular-weight peptides. Based on sensory analysis of EM, electrophoretic and RP-HPLC studies, hydrolyzates of 10% protein (degree of hydrolysis 5%; enzyme/ substrate E/S, 1:50) were finally incorporated into milk at 20% (v/v) to develop an acceptable product enriched with low-molecular-weight peptides. EM had higher protein content, viscosity and emulsifying properties than control milk with 3% fat. It is recommended that EM should not be sterilized as it results in coagulation, but can be safely pasteurized and spray dried without any undesirable effects. Maximum ACE-inhibition activity was obtained in hydrolyzate, followed by EM. This study is expected to boost the opportunity for the dairy industry to venture further into the nutraceutical dairy market.

Graphical abstract
Toward regional hazard risk assessment: a method to geospatially inventory critical coastal infrastructure applied to the Caribbean
Authors: Becker Austin , Hallisey Noah , Bove Gerald 
Published: May 2021

AbstractHurricanes and sea level rise pose significant threats to infrastructure and critical services (e.g., air and sea travel, water treatment), and can hinder sustainable development of major economic sectors (e.g., tourism, agriculture, and international commerce). Planning for a disaster-resilient future requires high-resolution, standardized data. However, few standardized approaches exist for identifying, inventorying, and quantifying infrastructure lands at risk from natural hazards. This research presents a cost effective, standardized and replicable method to geospatially inventory critical coastal infrastructure land use and components, for use in risk assessments or other regional analyses. While traditional approaches to geospatial inventorying rely on remote sensing or techniques, such as object-based image analysis (OBIA) to estimate land use, the current approach utilizes widely available satellite imagery and a “standard operating procedure” that guides individual mappers through the process, ensuring replicability and confidence. As a pilot study to develop an approach that can be replicated for other regions, this manuscript focuses on the Caribbean. Small islands rely heavily on a small number of critical coastal infrastructure (airports, seaports, power plants, water and wastewater treatment facilities) and climate related hazards threaten sustainable development and economic growth. The Caribbean is a large and diverse area, and gaps exist between countries in the resources required for planning but much of the region lacks a comprehensive inventory of the land, infrastructure, and assets at risk. Identifying and prioritizing infrastructure at risk is the first step towards preserving the region’s economy and planning for a disaster resilient future. This manuscript uses high resolution satellite imagery to identify and geo-spatially classify critical infrastructure land area and assets, such as structures, equipment, and impervious surfaces. We identified 386 critical coastal infrastructure facilities across 28 Caribbean nations/territories, with over 19,000 ha of coastal land dedicated to critical infrastructure. The approach establishes a new standard for the creation of geospatial data to assess land use change, risk, and other research questions suitable for the regional scale, but with sufficient resolution such that individual facilities can utilize the data for local-scale analysis.
2D-wavelet based micro and macro texture analysis for asphalt pavement under snow or ice condition
Authors: Li Feng , Ablat Gulnigar , Zhou Siqi , Liu Yixin , Bi Yufeng , Weng Zihang , Du Yuchuan 
Published: May 2021

AbstractIn ice and snow weather, the surface texture characteristics of asphalt pavement change, which will significantly affect the skid resistance performance of asphalt pavement. In this study, five asphalt mixture types of AC-5, AC-13, AC-16, SMA-13, SMA-16 were prepared under three conditions of the original state, ice and snow. In this paper, a 2D-wavelet transform approach is proposed to characterize the micro and macro texture of pavement. The Normalized Energy (NE) is proposed to describe the pavement texture quantitatively. Compared with the mean texture depth (MTD), NE has the advantages of full coverage, full automation and wide analytical scale. The results show that snow increases the micro-scale texture because of its fluffiness, while the formation of the ice sheets on the surface reduces the micro-scale texture. The filling effect of snow and ice reduces the macro-scale texture of the pavement surface. In a follow-up study, the 2D-wavelet transform approach can be applied to improve the intelligent driving braking system, which can provide pavement texture information for the safe braking strategy of driverless vehicles.
Annexin A5 as a marker for hepatocellular carcinoma in cirrhotic hepatitis C virus patients
Authors: Serag Waleed Mohamed , Elsayed Basem Eysa 
Published: May 2021

Abstract
Background
The tumorigenesis and development in a variety of cancers is reportedly encouraged via Annexin A5. The levels of Annexin A5 were tested in patients with or without HCC who were affected by liver cirrhosis. The objective of our study was to detect Annexin A5 levels in such patients in order to assess their function as an HCC marker. The longitudinal study comprised 91 cirrhotic HCV patients with and without HCC, and 20 healthy volunteers in the control group approved by the National Hepatology and Tropical Medicine Research Institute (NHTMRI) between March 2017 and August 2018.The serum levels Annexin A5 were found in all groups with ELISA. ANOVA, Mann-Whitney, and χ2 tests had been applied.

Results
High scales of Annexin A5 (3.89 + 0.85) were recorded for cirrhosis with HCC, either than cirrhotic patients without HCC (3.06 ± 0.88) (P = 0.041), and either than the control group (0.54 ± 0.11) (P < 0.001).

Conclusion
In HCV cirrhotic patients with and without HCC, AnxA5 can be used as HCC marker.
IL-28B single nucleotide polymorphism as a predictor of hepatocellular carcinoma after treatment of chronic hepatitis C patients with direct acting antivirals
Authors: Ahmed Nancy Abdel Fattah , Ali Ahmed Shawky Hassan , El Baz Rizk Ahmed , El-Zayyadi Islam Abd El-Hamid 
Published: May 2021

Abstract
Background
Hepatitis C virus (HCV) infection is considered one of the main causes of chronic liver diseases. HCV is responsible for 25% of HCC cases worldwide. DAAs represent an important step for HCV eradication. The aim was to study the role of IL-28B single nucleotide polymorphism in the prediction of HCC in patients with HCV-related cirrhosis after DAAs.

Results
This study was done at Mansoura Specialized Hospital: 50 cases HCC after DAAs, 50 cases without HCC after DAAs, and 100 controls. SNPs of the IL-28B gene were genotyped. There was an insignificant difference between HCC patients and the cirrhotic group as regards genotypes (p value = 0.26) and alleles (p value = 0.77). A significant association in SNP of IL-28B between healthy individuals and the cirrhotic group was detected. C C genotype (28%) and C T (64%) genotype were more prevalent in the healthy group than in the cirrhotic group 20% and 52% respectively while T T genotype was more prevalent in cirrhotic patients (28%) than controls (8%). C allele was protective against cirrhosis with 60% distribution in healthy individuals and 46% in the cirrhotic group. T allele was more prevalent in cirrhotic (54%) than the normal group (40%)

Conclusion
Although IL-28B SNP had a role in HCV-related cirrhosis progression, it did not predict the probability for HCC development following DAAs.
Study on the influence of slope height and angle on the factor of safety and shape of failure of slopes based on strength reduction method of analysis
Authors: Shiferaw Henok Marie 
Published: May 2021

Abstract
Background
Decreasing slope angle and slope height increases the slope factor of safety and can change the shape of likely slope failure. The increase in the factor of safety is at different rates, which can depend on soil type and slope geometry. Understanding the relationship between the slope height and angle decrease with the increase in factor of safety is vital to implement an efficient method of increasing factor of safety for slope stability problems. In addition, the shape of slope failure has to be observed thoroughly, not to increase the sliding mass of soil for a likely slope failure, even though the factor of safety has increased.

Results
Three homogeneous slopes of different soil characteristics were analyzed several times by changing the slope height and angle to determine the factor of safety. The shape of failure was also observed and recorded for each slope height and angle decrease. The analysis results indicated that decreasing slope angle increases the factor of safety nearly linearly while a decrease in height increases the factor of safety at a parabolic rate. Slope height decrease increased the factor of safety at a higher rate for the clay soil while slope angle decrease increased factor of safety at a higher rate for sandy soil compared to the other types of soils considered. The toe slide was observed in clayey and sandy clay soils at higher slopes while the base slide was observed at slopes whose height is less than 2 m. The slope slide was dominant on sandy soil at different slope heights and slope angles.

Conclusions
While the factor of safety of slopes had increased with slope height and angle decrease, the rate of increases and thus the efficiencies are different which depended on the type of soil and geometry of slope. The shape of failure also changed which might increase the sliding mass of soil. This can be risky if slope failure occurs due to unforeseen events. Using the slope height and angle decrease methods for slope stabilization should be thoroughly investigated to choose the most efficient method and also should be checked not to increase the sliding mass of soil for a possible slope failure.
Hepatoprotective activity of Phyllanthus niruri Linn. endophytes
Authors: Kodoli Radhika S. , Galatage Sunil T. , Killedar Suresh G. , Pishwikar Sachin A. , Habbu Prasanna V. , Bhagwat Durgacharan A. 
Published: May 2021

AbstractBackgroundThe liver is the vital organ which plays a major role in metabolism with numerous functions in the human beings such as protein synthesis, hormone production, and detoxification. Present research work is focused on hepatoprotective potential of chloroform (PNFC) and ethyl acetate (PNFEA) endophytic fractions fromPhyllanthus niruri Linn. against CCl4-induced hepatotoxicity in albino Wistar rats. To test our hypothesis, both endophytic fungal fractions were tested for vitro antioxidant and in vivo hepatoprotective activity. Serum biochemical parameters like SGOT, SGOT, SALP, cholesterol, bilirubin, and protein were estimated to assess hepatoprotective activity.ResultsGroup of rats treated with CCl4possess marked hepatic damage and oxidative stress which indicates that cellular leakage and loss of functional integrity of cell membrane in liver. PNFC and PNFEA fractions of endophyte fromPhyllanthus niruri Linn. stem have significantly reduced the elevated levels of biomarkers like SGPT, SGOT, SALP, bilirubin, cholesterol, and total protein in CCl4-induced hepatotoxicity in rats. The results obtained confirm hepatoprotective activity of endophytic fractions (PNFC and PNFEA) mediated through the stabilization of plasma membrane, repair of hepatic tissue damage, return of biochemical marker levels to normal, and regeneration of hepatocytes. Histopathological observations revealed improvement in the liver architecture after the treatment of secondary metabolites of endophytic fractions against CCl4-induced liver damage. Both fungal endophytes PNFC and PNFEA showed DPPH scavenging activity with IC50of 97.79 μg/ml and 108.40 μg/ml, respectively, and possess antioxidant potential. Presence of flavonoids in the both fractions of endophytes may be a possible reason for its antioxidant potential and identified asEurotium amstelodamistrain.ConclusionBoth fungal endophytes PNFC and PNFEA possess hepatoprotective potential due to the presence of secondary metabolites of fungi, i.e.,Eurotiumam stelodamistrain which support the claim endophytes and act as a potent biomedicine for treatment of various chronic diseases.
Detection of 6-monoacetylemorphine in hair sample of heroin addicts using gas chromatography–mass spectrometry and significance of rehabilitation program
Authors: Ghauri Mohsin Ahmad , Hassan Fahad , Hassan Younusa , Atif Naveel , Adnan Ahmad 
Published: May 2021

AbstractBackgroundThe analysis of hair samples for the detection of drugs has become one of the convincing strategies in the field of forensic toxicology. A large number of cases concerning heroin abuse or its byproducts have been documented under the Control of Narcotic Substances Act, 1997, over the past decade. The present study was carried out with an aim to evaluate the primary metabolite of heroin, i.e., 6-monoacetylemorphine (6-MAM), in hair samples of addicts and subjects undergoing rehabilitation, thereafter accessing the success rate of the rehabilitation program at the de-addiction center.ResultsHair samples were randomly collected from 20 regular heroin addicts and 20 heroin addicts from their past, from the rehabilitation center, of different age groups. Samples were cleaned, digested, and extracted using an alkaline digestion mediator methyl tertiary butyl ether, followed by quantification of 6-MAM via gas chromatography–mass spectrometry (GC–MS). The mean concentration of 6-MAM in regular heroin addicts detected was 7.80 ng/mg and 2.34 ng/mg in samples of subjects undergoing rehabilitation at the de-addiction center, respectively.ConclusionTraces of 6-MAM in the hair sample of heroin addicts can be efficiently detected days after the last intake of heroin. In addition to that, our findings also give an idea for future evaluating the approximate timeframe for detection of 6-MAM and/or other metabolites of heroin in the hair sample. However, in the future, by carefully analyzing the hair samples that can be taken from rehabilitation centers from target subjects at different time intervals, the exact duration of traceable quantity of 6-MAM can be determined in the hair sample. Finally, it can be concluded that there is a significant success rate of the rehabilitation program at de-addiction centers in connection with dragging the 6-MAM level from the body.