bluuebunny/crossref_metadata_2025_split huggingface dataset:
Prevalence of Methicillin-Resistant Staphylococcus aureus Isolated from Clinical Samples at Narayani Samudayik Hospital, Chitwan, Nepal
Authors: Adhikari Rama , Kshetri Bindu , Sharma Khem Narayan , Khanal Santosh , Panta Om Prakash , Sharma Suprina , Poudel Pramod 
Published: February 2021

Objectives: The main objective of this study was to determine the prevalence of Methicillin Resistance Staphylococcus aureus (MRSA) and MDR bacteria isolated from various clinical specimens from the patients attending Narayani Samudayik Hospital, Chitwan
Methods: A cross sectional study was carried in NPI-Narayani Samudayik Hospital, Chitwan from June to December 2017. Altogether, 3610 clinical specimens mainly pus, blood and urine were collected, streaked on Mannitol Salt Agar and Blood Agar and incubated at 37°C for 24 hours. The confirmed colonies of S. aureus were sub-cultured on Nutrient Agar. The antibiotic susceptibility pattern of all isolates S. aureus was determined by Kirby Bauer disc diffusion method. Isolates resistant to cefoxitin (30mcg) were confirmed as MRSA.
Result: Among 3610 total clinical samples, 17.6 % (635/3610) showed growth and 95(14.96%) S. aureus were isolated. Higher number of S. aureus was isolated from pus sample (93.15%). Out of 95 S. aureus isolates, 55 (57.89%) were identified as MRSA while 40 (42.10%) were MSSA. Vancomycin, ceftriaxone and chloramphenicol were found to be most effective antibiotic against isolates. Whereas, the least effective antibiotic was cefoxitin followed by amoxiclav, oxacillin and amoxicillin.
Conclusion: This study concludes that the overall prevalence of MRSA and MDR among the bacterial isolates is higher compared to other studies. So, it is recommended to monitor the antibiotic susceptibility pattern of pathogens regularly and study the epidemiology of such isolates.
IMPACT OF SERVICE QUALITY, ATMOSPHERE OF STORE, PRICE AND WORD OF MOUTH ON PURCHASE DECISIONS IN SWIWINGS, JEMBER
Authors: Pradana Perkasa Rendra , Firdaus Muhammad , Pudjanarso Amien 
Published: February 2021

Culinary business in JemberRegency is increasing and developing rapidly. This phenomenon causes the consumer's various culinary choices. With the existing of competition in culinary business, triggers the employers to move faster in attracting consumers. Swiwings Jember has to be able to take advantage of business opportunity and try hard to apply marketing strategy and factors that can impact the purchasing. This research aims to determine the influence of Service quality, Store atmosphere, Price and Word of Mouth toward the buyingdecision in Swiwings Jember. This type of reseacrh is quantitative reseacrh. The technique uses purposive sampling technique. The research sample used was 50 respondents.This study used tools of analysis such as: Instrument test, Classic Assumption test, and Multiple Linear Regression Analysis. Hyphotesis is done using the t and F test. It is know that using the t test of Service Quality, Store Atmospheric and Price does not have a partially significant effect on the Purchasing Decision, whereas Word of Mouth has a partially significant effect on the Purchasing Decision. It is know that using the F test Service Quality, Store Atmosphere, Price and Word of Mouth have the significant simultaneous effect in purchasing decision.
Keywords:Service Quality, Store’s Atmosphere, Price, Word of Mouth and Purchasing Decisions
PENGARUH HUBUNGAN INTERPERSONAL, LINGKUNGAN KERJA, DAN KECERDASAN EMOSIONAL TERHADAP KINERJA PEGAWAI PADA KANTOR KOMISI PEMILIHAN UMUM DAERAH (KPUD) KABUPATEN PARIGI MOUTONG
Authors: Rahman Abd 
Published: February 2021

Tujuan penelitian ini adalah untuk mengetahui dan menganalisis pengaruh hubungan interpersonal, lingkungan kerja dan kecerdasan emosional terhadap kinerja pegawai Kantor Komisi Pemilihan Umum Daerah (KPUD) Kabupaten Parigi Moutong. Populasi dalam penelitian ini adalah seluruh pegawai yang bekerja dilingkungan Kantor KPUD Kabupaten Parigi Moutong sebanyak 59 orang. Untuk mencapai tujuan tersebut ditarik sampel sebanyak 38 orang responden dengan metode Proporsional Random Sampling, dan alat analisis yang digunakan adalah regresi linear berganda.
Hasil penelitian ini menyimpulkan bahwa ketiga variabel bebas yakni hubungan interpersonal, lingkungan kerja dan kecerdasan emosional secara serempak berpengaruh positif dan signifikan terhadap kinerja pegawai Kantor Komisi Pemilihan Umum Daerah (KPUD) Kabupaten Parigi Moutong, serta berdasarkan hasil analisis disimpulkan bahwa kedua variabel bebas yakni hubungan interpersonal dan lingkungan kerja secara parsial berpengaruh signifikan terhadap kinerja pegawai Kantor Komisi Pemilihan Umum Daerah (KPUD) Kabupaten Parigi Moutong. Sedangkan salah satu variabel bebas yakni variabel kecerdasan emosional tidak berpengaruh terhadap kinerja pegawai Kantor Komisi Pemilihan Umum Daerah (KPUD) Kabupaten Parigi Moutong.

Kata Kunci: Hubungan Interpersonal, Lingkungan Kerja, Kecerdasan Emosional, Kinerja
Is aromatic terpenoid composition of grapes in Northwestern Iberian wine cultivars related to variation in VviDXS1 gene?
Authors: Royo Carolina , Carbonell-Bejerano Pablo , Torres-Pérez Rafael , Freire Luisa , Ibáñez Javier , Martínez-Zapater José Miguel , Vilanova Mar 
Published: February 2021

BACKGROUND: Monoterpenes and C13-norisoprenoids are key terpenoid compounds for wine aroma. The enzyme encoded by VviDXS1 participates in terpenoid biosynthesis in grapevine fruits and gain-of-function mutations in this gene lead to characteristic muscat aroma. OBJECTIVE: To assess for VviDXS1 contribution to aroma variation in Northwestern Iberian wine cultivars, we resequenced this gene in 111 cultivars and compared grape juice terpenic composition in 12 of them. METHODS: VviDXS1 was capture-targeted for resequencing with Illumina paired-end reads, SAMtools was used for variant calling and gene haplotypes were reconstructed with PHASE. Monoterpenes and C13-norisoprenoids were quantified in free and glycosidically-bound forms from grape juice by gas chromatography-mass spectrometry. RESULTS: Terpenic composition discriminated between muscat, terpenic and neutral profiles across cultivars. While the terpenic profile of Loureira and Albariño white cultivars was not associated with muscat-like mutations, Albariño carries a V34L substitution in VviDXS1 that is also present in other aromatic cultivars and was not reported before. Tempranillo and Cabernet Sauvignon red cultivars accumulated higher levels of C13-norisoprenoids, which was not associated with specific variation in VviDXS1. CONCLUSIONS: Apart from the uncharacterized substitution present in Albariño, findings suggest that terpenoid pathway-related genes other than VviDXS1 could contribute to the aromatic attributes of these cultivars.
Natural diversity in health related phytochemicals in Turkish tomatoes
Authors: Bakir Sena , Capanoglu Esra , Hall Robert D. , de Vos Ric C.H. 
Published: February 2021

BACKGROUND: Tomatoes are important fruits in the Mediterranean diet and are considered to reduce the risk of different human diseases due to their antioxidative powers. OBJECTIVE: The purpose of the present study was to investigate the biodiversity within a set of 50 tomato fruit accessions collected across Turkey concerning their antioxidant capacities and the levels of potential health-beneficial compounds including phenolic compounds, carotenoids, ascorbic acid and tocopherols. METHODS: All accessions were simultaneously grown in an open experimental field in 2017 and ripe fruits were harvested for analysis. Antioxidant capacities of both hydrophilic and lipophilic extracts were determined using spectrophotometric assays after which individual antioxidants were identified by HPLC using an on-line antioxidant detection system. Phenolic acids, flavonoids, carotenoids and vitamins C and E were quantified using HPLC. RESULTS: The results indicated that there is a wide diversity within this small collection with respect to their hydrophilic and lipophilic antioxidants. CONCLUSION: Hydrophilic antioxidant capacity of the tomatoes was generally related to chlorogenic acid and Vitamin C levels, while lipophilic antioxidants were correlated to all-trans lycopene.
Sifat Spiritual Ibu Bapa dan Estim Kendiri dalam Kalangan Pelajar Universiti (The Spiritual Characteristics of Parents and Self-Esteem in Universities Students)
Authors: Adiha Tewiran Syaliza 
Published: February 2021

Sifat spiritual ibu bapa dalam mendidik anak-anak memberi impak yang sangatbesar bukan sahaja kepada hubungan kekeluargaan malah kepada personaliti anak itu sendiri apabila dewasa kelak. Kajian ini bertujuan mengenal pasti hubungan antara sifat spiritual ibu bapa dengan estim kendiri pelajar Fakulti Pengajian Pendidikan, UPM. Responden terdiri daripada 205 orang pelajar dipilih mengikut kaedah persampelan kluster. Instrumen yang digunakan iaitu soal selidik Parental Spiritual Characteristics Scales (PSCS) dan RosenbergSelf-Esteem Scale (RSES). Ujian statistik yang digunakan ialah kaedah analisis diskriptif dan analisis Pearson Correlation. Hasil kajian menunjukkan terdapat hubungan sifat spiritual ibu bapa dengan estim kendiri pelajar Fakulti Pengajian Pendidikan, UPM. Kajian ini turut memberikan manfaat kepada pihak universiti, ibu bapa dan kaunselor dalam memahami aspek-aspek sifat spiritual ibu bapa dan estim kendiri pelajar. Abstract The spiritual nature of parents in educating children has a tremendous impact not only on family relationships but also on the child’s own personality when they become adults. The purpose of this study is to identify the spiritual characteristics of parents who are often practiced with the students’ selfesteem of the Faculty of Educational Studies, UPM. Respondents were 205 students, selected according to cluster sampling method. The instruments used were Parental Spiritual Characteristics Scales (PSCS) and Rosenberg Self-Esteem Scale (RSES). The statistical test used is descriptive analysis and Pearson Correlation. The results showed that there was a relationship between the parents’ spiritual character and the self-esteem of the Faculty of Educational Studies, UPM. This study also benefits the university, parents and counselors in understanding aspects of parent’s spiritual traits and student self- esteem. Keywords: Spiritual characteristics, self- esteem, parent-child correlation.
Experience of healthcare workers in combatting COVID-19 in Indonesia: A descriptive qualitative study
Authors: Tosepu Ramadhan , Gunawan Joko , Effendy Devi Savitri , Rustam HN Muhammad , Muchtar Febriana , Sakka Ambo , Indriastuti Diah 
Published: February 2021

Background: The number of COVID-19 cases in Indonesia continues to rise. The roles and responsibilities of healthcare workers are crucial in the battle of the COVID-19.
Objective: This study aimed to explore the experience of healthcare workers in combatting COVID-19 in some parts of Indonesia.
Methods: This study employed a descriptive qualitative study design. Online semi-structured interviews were conducted in April 2020 among thirteen participants who were purposively selected. Data were analyzed using thematic analysis.
Results: Eight themes emerged from data, including lack of personal protective equipment, lack of referral hospitals and limited facilities, dealing with patients with unknown status, feeling worried about getting infected or being a source of viral transmission, being creative, too long shift and fatigue, being surrender to God, and government issues.
Conclusion: Findings of this study could be used as the input for the government, healthcare workers, and the general population in combatting COVID-19.
El modelo sistémico de aprendizaje y enseñanza, como apoyo en la inserción laboral
Authors: Corcino-Barrueta Fernando E. , Chamoli-Falcon Andy W. , Otalora-Martinez Claudia R. , Melgarejo-Figueroa Maria Del Pilar 
Published: February 2021

La investigación tuvo como objetivo principal buscar cómo influye el proceso sistémico con el enfoque de enseñanza y aprendizaje, en la inclusión laboral de los egresados de la Facultad de Derecho de la Universidad Nacional Hermilio Valdizán. Para lograrlo, se desarrolló el estudio en base a tipo descriptivo explicativo, bajo un diseño no experimental, correlacional y de tipo transversal, para lo cual se trabajó con una población de 121 egresados y la muestra estuvo constituida por 37 egresados de los años 2016 y 2017, siendo del tipo no probabilístico, los cuales se construyeron dos cuestionarios, un cuestionario para medir la variable proceso sistemático de enseñanza-aprendizaje, otro cuestionario para medir la variable oportunidad de inserción laboral, todo ello para calificar el nivel de influencia. Para evaluar la hipótesis se aplicó la prueba Ro de Sperman. A raíz de ello nuestros resultados confirmaron la hipótesis de estudio, demostrando que el proceso sistémico de enseñanza-aprendizaje muestra una relación significativa con la inserción laboral y que existe influencia positiva entre ambas variables. Llegando a la conclusión que ejercer un proceso sistémico en la enseñanza y aprendizaje genera oportunidad en la inclusión laboral.
Проблеми адміністративної відповідальності за порушення правил щодо карантину людей
Authors: Мотиль В. І. , Дякін Я. О. 
Published: February 2021

У статті досліджуються актуальні проблеми адміністративної відповідальності за порушення правил карантину людей, пов’язані насамперед з бланкетною диспозицією ст. 44-3 КУпАП, спільними рисами форм об’єктивної сторони адміністративного правопорушення, передбаченого ст. 44-3 КУпАП, та злочину, передбаченого ст. 325 КК України, а також обмеженням гарантованих Конституцією прав та свобод людини й громадянина через запровадження на території України окремих правил карантину у зв՚язку зі стрімким поширенням гострої респіраторної хвороби COVID-19, спричиненої коронавірусом SARS-CoV-2. Розроблено пропозиції щодо вирішення зазначених проблем, зокрема шляхом диференціації адміністративної відповідальності за вказані діяння, створення чіткого переліку нормативно-правових актів порушення яких може мати наслідком притягнення до адміністративної відповідальності за певні діяння, а також запровадження відповідних заборон у суворій згідності з Конституцією України, зокрема після введення надзвичайного стану або внесення відповідних змін до Основного закону української держави.
Monitoring Systems for Checking Websites on Accessibility
Authors: Burkard Andreas , Zimmermann Gottfried , Schwarzer Bettina 
Published: February 2021

Web accessibility monitoring systems support users in checking entire websites for accessibility issues. Although these tools can only check the compliance with some of the many success criteria of the Web Content Accessibility Guidelines, they can assist quality assurance personnel, web administrators and web authors to discover hotspots of barriers and overlooked accessibility issues in a continuous manner. These tools should be effective in identifying accessibility issues. Furthermore, they should motivate users, as this promotes employee productivity and increases interest in accessibility in general. In a comparative study, we applied four commercial monitoring systems on two of the Stuttgart Media University’s websites. The tools are: 1) The Accessibility module of Siteimprove from Siteimprove, 2) Pope Tech from Pope Tech, 3) WorldSpace Comply (now called axe Monitor) from Deque, and 4) ARC Monitoring from The Paciello Group. The criteria catalogue consists of functional criteria that we gleaned from literature and user experience criteria based on the User Experience Questionnaire. Based on a focus group consisting of experts of Stuttgart Media University, we derived individual weights for the criteria. The functional evaluation criteria are: Coverage of the website and the guidelines, completeness, correctness, support in locating errors, support for manual checks, degree of implementing gamification patterns, support for various input and report formats, and methodological support for the Website Accessibility Conformance Evaluation Methodology 1.0 and for the German procurement law for public authorities Barrierefreie Informationstechnik-Verordnung 2.0. For determination of the user experience criteria, we conducted exploratory think-aloud user tests (n = 15) using a coaching approach. Every participant tested all tools for 15 min (within-subject design). The participants completed post-test questionnaires, including the User Experience Questionnaire. According to our results, Siteimprove turned out to be the best tool for our purposes.
Comprehensive Analysis of Tumor Microenvironment Identified Prognostic Immune-Related Gene Signature in Ovarian Cancer
Authors: Li Na , Li Biao , Zhan Xianquan 
Published: February 2021

BackgroundAccumulating evidence demonstrated that tumor microenvironmental cells played important roles in predicting clinical outcomes and therapeutic efficacy. We aimed to develop a reliable immune-related gene signature for predicting the prognosis of ovarian cancer (OC).MethodsSingle sample gene-set enrichment analysis (ssGSEA) of immune gene-sets was used to quantify the relative abundance of immune cell infiltration and develop high- and low-abundance immune subtypes of 308 OC samples. The presence of infiltrating stromal/immune cells in OC tissues was calculated as an estimate score. We estimated the correlation coefficients among the immune subtype, clinicopathological feature, immune score, distribution of immune cells, and tumor mutation burden (TMB). The differentially expressed immune-related genes between high- and low-abundance immune subtypes were further used to construct a gene signature of a prognostic model in OC with lasso regression analysis.ResultsThe ssGSEA analysis divided OC samples into high- and low-abundance immune subtypes based on the abundance of immune cell infiltration, which was significantly related to the estimate score and clinical characteristics. The distribution of immune cells was also significantly different between high- and low-abundance immune subtypes. The correlation analysis showed the close relationship between TMB and the estimate score. The differentially expressed immune-related genes between high- and low-abundance immune subtypes were enriched in multiple immune-related pathways. Some immune checkpoints (PDL1, PD1, and CTLA-4) were overexpressed in the high-abundance immune subtype. Furthermore, the five-immune-related-gene-signature prognostic model (CCL18, CXCL13, HLA-DOB, HLA-DPB2, and TNFRSF17)-based high-risk and low-risk groups were significantly related to OC overall survival.ConclusionImmune-related genes were the promising predictors of prognosis and survival, and the comprehensive landscape of tumor microenvironmental cells of OC has potential for therapeutic schedule monitoring.
CFH I62V as a Putative Genetic Marker for Posner-Schlossman Syndrome
Authors: Yang Ming Ming , Sun Hong Yan , Meng Ting , Qiu Shan Hu , Zeng Qi Qiao , Ng Tsz Kin , Jiang Li , Deng Ting Ming , Zeng Ai Neng , Wang Jun , Luo Xiao Ling 
Published: February 2021

Objective: Posner-Schlossman syndrome (PSS), also known as glaucomatocyclitic crisis, is an ocular condition characterized by recurrent attacks of anterior uveitis and raised intraocular pressure. Previous studies by our team and others have identified the genetic association of complement pathway genes with uveitis and glaucoma. This study aimed to investigate the complement genes in PSS patients with the view of elucidating the genetic background of the disease.Methods: A total of 331 subjects (56 PSS patients and 275 controls) were recruited for this study. We selected 27 variants in six complement pathway genes (SERPING1, C2, CFB, CFH, C3, and C5) and detected them using TaqMan single nucleotide polymorphism (SNP) Genotyping Assays. Univariate SNP association analysis, haplotype-based association analysis, gene-gene interaction analysis among complement genes, and genotype-phenotype correlation analysis were performed.Results: Among the 27 variants of six complement pathway genes, the functional variant I62V (rs800292) at the CFH gene was found to be significantly associated with PSS; there was a significant increase in the frequency of A allele and AA homozygosity in PSS patients than in controls (P = 1.79 × 10−4; odds ratio (OR) 2.18, 95% CI: 1.44–3.29; P = 4.65 × 10−4; OR 3.66, 95% CI: 1.70–7.85, respectively). The additive effect of CFH-rs800292 and SERPING1-rs3824988 was identified with an OR of 12.50 (95% CI: 2.16–72.28). Genotype-phenotype analysis indicated that the rs800292 AA genotype was associated with a higher intraocular pressure and higher frequency of recurrence. Unlike a high proportion of human leukocyte antigen (HLA)-B27 positivity in anterior uveitis, only 3 in 56 (5.36%) PSS patients were HLA-B27 positive. In addition, one haplotype block (GC) in the SERPING1 gene showed a nominal association with PSS with an increased risk of 2.04 (P = 0.01; 95% CI: 1.18–3.53), but the P-value could not withstand the Bonferroni correction (Pcorr &gt; 0.05).Conclusion: This study revealed a genetic association of a CFH variant with PSS as well as its clinical parameters, implying that the alternative complement pathway might play an important role in the pathogenesis of PSS. Further studies to enrich the understanding of the genetic background of PSS and the role of the complement system in ocular inflammation are warranted.
Predominance of Non-carbapenemase Producing Carbapenem-Resistant Enterobacterales in South Texas
Authors: Black Cody A. , So Wonhee , Dallas Steven S. , Gawrys Gerard , Benavides Raymond , Aguilar Samantha , Chen Chang-Jui , Shurko James F. , Lee Grace C. 
Published: February 2021

BackgroundCarbapenem-resistant Enterobacterales (CRE) pose a significant global public health threat. Resistance among CRE is particularly complex, owing to numerous possible resistance mechanisms and broad definitions. We aimed to characterize the clinical and molecular profiles of CRE in the South Texas region.Materials and methodsWe compared the clinical, genotypic, and phenotypic profiles of carbapenemase producing Enterobacterales (CPE) with those of non-carbapenemase producers (NCPE) isolated from South Texas, United States between 2011 and 2019. Molecular characteristics and resistance mechanisms were analyzed using whole-genome sequences.ResultsThe majority (59%) of the CRE isolates were NCPE while 41% of isolates harbored carbapenemases, predmonantly blaKPC-type. The most common CPE was Klebsiella pneumoniae while majority of Enterobacter cloacae and Escherichia coli were NCPE Among K. pneumoniae, the clonal group 307 has emerged as a predmoninant group and was associated with as many CRE infections as the previous common clonal group 258. Patients with NCPE compared to CPE infections were associated with higher antimicrobial exposure prior to culture collection (days of therapy, 795 vs. 242; p &lt; 0.001) and emergency department visits within past 90 days (22% vs. 4%; p = 0.011). The all cause 30-day mortality was 21%.ConclusionsThis study highlights the diversity of resistance mechanisms underlying CRE in South Texas, with 59% not harboring a carbapenemase. Individuals with NCPE infections were more likely to have had prior antimicrobial therapy and emergency department visits compared to those with CPE. Identification and distinction of these mechanisms by rapid identification of species and carbapenemase would allow for optimal treatment and infection control efforts.