bluuebunny/crossref_metadata_2025_split huggingface dataset:
Diatom Hotspots Driven by Western Boundary Current Instability
Authors: Oliver Hilde , Zhang Weifeng G. , Smith Walker O. , Alatalo Philip , Chappell P. Dreux , Hirzel Andrew J. , Selden Corday R. , Sosik Heidi M. , Stanley Rachel H. R. , Zhu Yifan , McGillicuddy Dennis J. 
Published: May 2021

AbstractClimatic changes have decreased the stability of the Gulf Stream (GS), increasing the frequency at which its meanders interact with the Mid‐Atlantic Bight (MAB) continental shelf and slope region. These intrusions are thought to suppress biological productivity by transporting low‐nutrient water to the otherwise productive shelf edge region. Here we present evidence of widespread, anomalously intense subsurface diatom hotspots in the MAB slope sea that likely resulted from a GS intrusion in July 2019. The hotspots (at ∼50 m) were associated with water mass properties characteristic of GS water (∼100 m); it is probable that the hotspots resulted from the upwelling of GS water during its transport into the slope sea, likely by a GS meander directly intruding onto the continental slope east of where the hotspots were observed. Further work is required to unravel how increasingly frequent direct GS intrusions could influence MAB marine ecosystems.
High Latitude Zones of GeV Heavy Ions at the Inner Edge of Jupiter's Relativistic Electron Belt
Authors: Becker Heidi N. , Alexander James W. , Connerney John E. P. , Brennan Martin J. , Guillaume Alexandre , Adumitroaie Virgil , Florence Meghan M. , Kollmann Peter , Mauk Barry H. , Bolton Scott J. 
Published: May 2021

AbstractJuno has discovered signatures of a high energy heavy ion population (>100 MeV/nucleon) within the inner edge of Jupiter's relativistic electron belt. The particles were detected in narrow zones in the high‐latitude lobes of the synchrotron emission region, a location never explored by prior spacecraft (∼31°–46° magnetic latitude; radial distances 1.12–1.41 Jovian radii, M‐shells 1.5–2.37). The population was revealed by extremely high signal ionization signatures seen in images collected by Juno's Stellar Reference Unit star camera (equivalent to 0.2–0.7 MeV of deposited energy per pixel). Signature morphology indicates a population of GeV ions (Z > 1) with atomic mass as high as sulfur, although species with 2 ≤ Z ≤ 8 potentially account for all of the signatures. The detections have occurred while the spacecraft is magnetically connected to Jupiter's halo and gossamer rings, but not while it is connected to the main ring; similar to equatorial observations of GeV carbon and sulfur ions by the Galileo Probe. We find that the particles reside on magnetic drift shells that suggest they are stably trapped within Jupiter's magnetosphere. Because the cosmic ray albedo neutron decay mechanism can only produce protons and electrons, it cannot be the source of this trapped Z > 1 ion population; other galactic cosmic ray origins are suspected. Inward radial transport of tens of keV heavy ions from the outer magnetosphere is another potential source.
Simultaneously controlling heat conduction and infrared absorption with a textured dielectric film to enhance the performance of thermopiles
Authors: He Yunqian , Wang Yuelin , Li Tie 
Published: May 2021

AbstractThe heat conduction and infrared absorption properties of the dielectric film have a great influence on the thermopile performance. Thinning the dielectric film, reducing its contact area with the silicon substrate, or adding high-absorptivity nanomaterials has been proven to be effective in improving thermopiles. However, these methods may result in a decrease in the structural mechanical strength and increases in the fabrication complexity and cost. In this work, a new performance-enhancement strategy for thermopiles by simultaneously controlling the heat conduction and infrared absorption with a TExtured DIelectric (TEDI) film is developed and presented. The TEDI film is formed in situ by a simple hard-molding process that is compatible with the fabrication of traditional thermopiles. Compared to the control FLat DIelectric (FLDI) film, the intrinsic thermal conductance of the TEDI film can be reduced by ~18–30%, while the infrared absorption can be increased by ~7–13%. Correspondingly, the responsivity and detectivity of the fabricated TEDI film-based thermopile can be significantly enhanced by ~38–64%. An optimized TEDI film-based thermopile has achieved a responsivity of 156.89 V·W−1 and a detectivity of 2.16 × 108 cm·Hz1/2·W−1, while the response time constant can remain <12 ms. These results exhibit the great potential of using this strategy to develop high-performance thermopiles and enhance other sensors with heat transfer and/or infrared absorption mechanisms.
Viruses infecting a warm water picoeukaryote shed light on spatial co-occurrence dynamics of marine viruses and their hosts
Authors: Bachy Charles , Yung Charmaine C M , Needham David M , Gazitúa Maria Consuelo , Roux Simon , Limardo Alexander J , Choi Chang Jae , Jorgens Danielle M , Sullivan Matthew B , Worden Alexandra Z 
Published: May 2021

Abstract
The marine picoeukaryote Bathycoccus prasinos has been considered a cosmopolitan alga, although recent studies indicate two ecotypes exist, Clade BI (B. prasinos) and Clade BII. Viruses that infect Bathycoccus Clade BI are known (BpVs), but not that infect BII. We isolated three dsDNA prasinoviruses from the Sargasso Sea against Clade BII isolate RCC716. The BII-Vs do not infect BI, and two (BII-V2 and BII-V3) have larger genomes (~210 kb) than BI-Viruses and BII-V1. BII-Vs share ~90% of their proteins, and between 65% to 83% of their proteins with sequenced BpVs. Phylogenomic reconstructions and PolB analyses establish close-relatedness of BII-V2 and BII-V3, yet BII-V2 has 10-fold higher infectivity and induces greater mortality on host isolate RCC716. BII-V1 is more distant, has a shorter latent period, and infects both available BII isolates, RCC716 and RCC715, while BII-V2 and BII-V3 do not exhibit productive infection of the latter in our experiments. Global metagenome analyses show Clade BI and BII algal relative abundances correlate positively with their respective viruses. The distributions delineate BI/BpVs as occupying lower temperature mesotrophic and coastal systems, whereas BII/BII-Vs occupy warmer temperature, higher salinity ecosystems. Accordingly, with molecular diagnostic support, we name Clade BII Bathycoccus calidus sp. nov. and propose that molecular diversity within this new species likely connects to the differentiated host-virus dynamics observed in our time course experiments. Overall, the tightly linked biogeography of Bathycoccus host and virus clades observed herein supports species-level host specificity, with strain-level variations in infection parameters.
Peanut and hazelnut occurrence as allergens in foodstuffs with precautionary allergen labeling in Canada
Authors: Manny Emilie , La Vieille Sébastien , Barrere Virginie , Théolier Jérémie , Godefroy Samuel Benrejeb 
Published: May 2021

AbstractPrecautionary allergen labeling (PAL) is widely used by food industries. Occurrence studies revealed that few analyzed products contained the allergen(s) present in the statement, but little is known in Canada. To improve manufacturing practices and better manage allergen cross-contamination, occurrence data is needed to determine the exposure of allergic individuals eating those products. Samples were analyzed for peanuts (n = 871) and hazelnuts (n = 863) using ELISA methods. Within samples analyzed for peanuts, 72% had a PAL (n = 628), 1% had peanuts as a minor ingredient (n = 9) and 27% were claimed “peanut-free” (n = 234). Most hazelnut samples had a PAL for tree nuts/hazelnuts (94%; n = 807) with 6% claimed “nut-free” (n = 56). Peanuts and hazelnuts were found in 4% (0.6–28.1 ppm) and 9% (0.4–2167 ppm) of all samples, respectively. Chocolates were mostly impacted; they should be treated apart from other foods and used in risk assessments scenarios to improve manufacturing practices, reducing unnecessary PAL use.
Multimodal integrated approaches in low grade glioma surgery
Authors: Ius Tamara , Mazzucchi Edoardo , Tomasino Barbara , Pauletto Giada , Sabatino Giovanni , Della Pepa Giuseppe Maria , La Rocca Giuseppe , Battistella Claudio , Olivi Alessandro , Skrap Miran 
Published: May 2021

AbstractSurgical management of Diffuse Low-Grade Gliomas (DLGGs) has radically changed in the last 20 years. Awake surgery (AS) in combination with Direct Electrical Stimulation (DES) and real-time neuropsychological testing (RTNT) permits continuous intraoperative feedback, thus allowing to increase the extent of resection (EOR). The aim of this study was to evaluate the impact of the technological advancements and integration of multidisciplinary techniques on EOR. Two hundred and eighty-eight patients affected by DLGG were enrolled. Cases were stratified according to the surgical protocol that changed over time: 1. DES; 2. DES plus functional MRI/DTI images fused on a NeuroNavigation system; 3. Protocol 2 plus RTNT. Patients belonging to Protocol 1 had a median EOR of 83% (28–100), while those belonging to Protocol 2 and 3 had a median EOR of 88% (34–100) and 98% (50–100) respectively (p = 0.0001). New transient deficits with Protocol 1, 2 and 3 were noted in 38.96%, 34.31% and 31,08% of cases, and permanent deficits in 6.49%, 3.65% and 2.7% respectively. The average follow-up period was 6.8 years. OS was influenced by molecular class (p = 0.028), EOR (p = 0.018) and preoperative tumor growing pattern (p = 0.004). Multimodal surgical approach can provide a safer and wider removal of DLGG with potential subsequent benefits on OS. Further studies are necessary to corroborate our findings.
Leaf trait variation in species-rich tropical Andean forests
Authors: Homeier Jürgen , Seeler Tabea , Pierick Kerstin , Leuschner Christoph 
Published: May 2021

AbstractScreening species-rich communities for the variation in functional traits along environmental gradients may help understanding the abiotic drivers of plant performance in a mechanistic way. We investigated tree leaf trait variation along an elevation gradient (1000–3000 m) in highly diverse neotropical montane forests to test the hypothesis that elevational trait change reflects a trend toward more conservative resource use strategies at higher elevations, with interspecific trait variation decreasing and trait integration increasing due to environmental filtering. Analysis of trait variance partitioning across the 52 tree species revealed for most traits a dominant influence of phylogeny, except for SLA, leaf thickness and foliar Ca, where elevation was most influential. The community-level means of SLA, foliar N and Ca, and foliar N/P ratio decreased with elevation, while leaf thickness and toughness increased. The contribution of intraspecific variation was substantial at the community level in most traits, yet smaller than the interspecific component. Both within-species and between-species trait variation did not change systematically with elevation. High phylogenetic diversity, together with small-scale edaphic heterogeneity, cause large interspecific leaf trait variation in these hyper-diverse Andean forests. Trait network analysis revealed increasing leaf trait integration with elevation, suggesting stronger environmental filtering at colder and nutrient-poorer sites.
Tiny, ornamented eggs and eggshell from the Upper Cretaceous of Utah represent a new ootaxon with theropod affinities
Authors: Oser Sara E. , Chin Karen , Sertich Joseph J. W. , Varricchio David J. , Choi Seung , Rifkin Jeffrey 
Published: May 2021

AbstractA new Cretaceous ootaxon (eggshell type) from the Kaiparowits Formation of Grand Staircase-Escalante National Monument is among a growing number of very small eggs described from the Mesozoic. Analyses of two partial eggs (~ 17.7 mm in diameter) and 29 eggshell fragments reveal that this new ootaxon exhibits nodose ornamentation with distinctive branching pore canals that open atop the nodes. Its two-layered microstructure consists of a mammillary layer and a continuous layer with rugged grain boundaries between calcite grains. Although the exact identity of the egg producer is unknown, the eggshell microstructure and small size is consistent with a small-bodied avian or non-avian theropod. The specific combination of small egg size, branching pores, two-layered microstructure, and dispersituberculate ornamentation preserved in this new ootaxon is unique among theropod eggs. This underscores that both eggshell and skeletal fossils of Cretaceous theropods can display a mosaic of transitional morphological and behavioural features characteristic of both avian and non-avian taxa. As such, this new ootaxon increases the diversity of Cretaceous eggs and informs our understanding of the evolution of theropod eggshell microstructure and morphology.
The SoftWipe tool and benchmark for assessing coding standards adherence of scientific software
Authors: Zapletal Adrian , Höhler Dimitri , Sinz Carsten , Stamatakis Alexandros 
Published: May 2021

AbstractScientific software from all areas of scientific research is pivotal to obtaining novel insights. Yet the coding standards adherence of scientific software is rarely assessed, even though it might lead to incorrect scientific results in the worst case. Therefore, we have developed an open source tool and benchmark called , that provides a relative software coding standards adherence ranking of 48 computational tools from diverse research areas. can be used in the review process of software papers and to inform the scientific software selection process.
Erosion of tropical bird diversity over a century is influenced by abundance, diet and subtle climatic tolerances
Authors: Curtis Jenna R. , Robinson W. Douglas , Rompré Ghislain , Moore Randall P. , McCune Bruce 
Published: May 2021

AbstractHuman alteration of landscapes leads to attrition of biodiversity. Recommendations for maximizing retention of species richness typically focus on protection and preservation of large habitat patches. Despite a century of protection from human disturbance, 27% of the 228 bird species initially detected on Barro Colorado Island (BCI), Panama, a large hilltop forest fragment isolated by waters of Gatun Lake, are now absent. Lost species were more likely to be initially uncommon and terrestrial insectivores. Analyses of the regional avifauna, exhaustively inventoried and mapped across 24 subregions, identified strong geographical discontinuities in species distributions associated with a steep transisthmian rainfall gradient. Having lost mostly species preferring humid forests, the BCI species assemblage continues to shift from one originally typical of wetter forests toward one now resembling bird communities in drier forests. Even when habitat remnants are large and protected for 100 years, altered habitat characteristics resulting from isolation produce non-random loss of species linked with their commonness, dietary preferences and subtle climatic sensitivities.
Increased disease activity in early arthritis patients with anti-carbamylated protein antibodies
Authors: Regueiro Cristina , Nuño Laura , Triguero-Martinez Ana , Ortiz Ana M. , Villalba Alejandro , Bóveda María Dolores , Martínez-Feito Ana , Conde Carmen , Balsa Alejandro , González-Alvaro Isidoro , Gonzalez Antonio 
Published: May 2021

AbstractThe initial management of rheumatoid arthritis (RA) has a high impact on disease prognosis. Therefore, we need to select the most appropriate treatment as soon as possible. This goal requires biomarkers of disease severity and prognosis. One such biomarker may be the presence of anti-carbamylated protein antibodies (ACarPA) because it is associated with adverse long term outcomes as radiographic damage and mortality. Here, we have assessed the ACarPA as short-term prognostic biomarkers. The study was conducted in 978 prospective early arthritis (EA) patients that were followed for two years. Our results show the association of ACarPA with increased levels of all the disease activity measures in the first visit after arthritis onset. However, the associations were more significant with the high levels in local measures of inflammation and physician assessment than with the increases in systemic inflammation and patient-reported outcomes. More notably, disease activity was persistently increased in the ACarPA positive patients during the two years of follow-up. These differences were significant even after accounting for the presence of other RA autoantibodies. Therefore, the ACarPA could be considered short-term prognostic biomarkers of increased disease activity in the EA patients.
Detecting infected asymptomatic cases in a stochastic model for spread of Covid-19: the case of Argentina
Authors: Barreiro N. L. , Govezensky T. , Bolcatto P. G. , Barrio R. A. 
Published: May 2021

AbstractWe have studied the dynamic evolution of the Covid-19 pandemic in Argentina. The marked heterogeneity in population density and the very extensive geography of the country becomes a challenge itself. Standard compartment models fail when they are implemented in the Argentina case. We extended a previous successful model to describe the geographical spread of the AH1N1 influenza epidemic of 2009 in two essential ways: we added a stochastic local mobility mechanism, and we introduced a new compartment in order to take into account the isolation of infected asymptomatic detected people. Two fundamental parameters drive the dynamics: the time elapsed between contagious and isolation of infected individuals ($$\alpha$$
α
) and the ratio of people isolated over the total infected ones (p). The evolution is more sensitive to the $$p-$$

p
-

parameter. The model not only reproduces the real data but also predicts the second wave before the former vanishes. This effect is intrinsic of extensive countries with heterogeneous population density and interconnection.The model presented has proven to be a reliable predictor of the effects of public policies as, for instance, the unavoidable vaccination campaigns starting at present in the world an particularly in Argentina.
The large extracellular loop of CD63 interacts with gp41 of HIV-1 and is essential for establishing the virological synapse
Authors: Ivanusic Daniel , Madela Kazimierz , Bannert Norbert , Denner Joachim 
Published: May 2021

AbstractHuman immunodeficiency virus type 1 (HIV-1) persists lifelong in infected individuals and has evolved unique strategies in order to evade the immune system. One of these strategies is the direct cell-to-cell spread of HIV-1. The formation of a virological synapse (VS) between donor and target cell is important for this process. Tetraspanins are cellular proteins that are actively involved in the formation of a VS. However, the molecular mechanisms of recruiting host proteins for the cell–cell transfer of particles to the VS remains unclear. Our study has mapped the binding site for the transmembrane envelope protein gp41 of HIV-1 within the large extracellular loop (LEL) of CD63 and showed that this interaction occurs predominantly at the VS between T cells where viral particles are transferred. Mutations within the highly conserved CCG motif of the tetraspanin superfamily abrogated recruiting of expressed HIV-1 GFP fused Gag core protein and CD63 to the VS. This demonstrates the biological significance of CD63 for enhanced formation of a VS. Since cell–cell spread of HIV-1 is a major route of persistent infection, these results highlight the central role of CD63 as a member of the tetraspanin superfamily during HIV-1 infection and pathogenesis.
Carbon nanoparticle suspension could help get a more accurate nodal staging for patient with rectal cancer
Authors: Ge Wei , Li Qiang , Liu Wen-jia , Zhang Xiao-Qi , Fan Xiang-shan , Shao Li-hua , Tao Liang , Guan Wen-xian , Chen Gang 
Published: May 2021

AbstractThis study aimed to evaluate whether carbon nanoparticles could improve the accuracy of nodal staging in colorectal cancer (CRC). We performed a randomized controlled trial with CRC at the department of general surgery, the affiliated hospital of Nanjing University Medical School. A total of 160 patients were recruited in this research and 132 patients were included in the safety analyses. Among these patients, 72 cases were classified into control group and 60 cases into study group. The mean number of lymph nodes harvested from patients in study group was 19.3 ± 6.7 (range from 4 to 38), which was higher than that in control group (15.1 ± 5.7 (range from 3 to 29)) (p < 0.001). The mean number of positive lymph nodes got from patients in study group was 1.7 ± 3.5 (range from 0 to 22), which was also higher than that in control group (0.7 ± 1.4 (range from 0 to 7)) (p = 0.045). In study group, there were 30 patients (50%) proved to be N0, and remaining 30 patients (50%) were N1 or N2. However, 50 patients (69.4%) were N0 and 22 patients (30.6%) were N1 or N2 in control group. The rate of N0 in control group was significantly higher than that in study group (p = 0.023). Injecting carbon nanoparticle suspension could get a more accurate nodal staging to receive enough chemoradiotherapy, improving prognosis. Besides, injecting carbon nanoparticles suspension at four points 5 cm, 10 cm, 15 cm and 20 cm away from the anus by “sandwich” method was a new try.Trial registration: This study was registered with ClinicalTrials.gov, number ChiCTR1900025127 on 12/8/2019.
Associative learning and extinction of conditioned threat predictors across sensory modalities
Authors: Koenen Laura. R. , Pawlik Robert. J. , Icenhour Adriane , Petrakova Liubov , Forkmann Katarina , Theysohn Nina , Engler Harald , Elsenbruch Sigrid 
Published: May 2021

AbstractThe formation and persistence of negative pain-related expectations by classical conditioning remain incompletely understood. We elucidated behavioural and neural correlates involved in the acquisition and extinction of negative expectations towards different threats across sensory modalities. In two complementary functional magnetic resonance imaging studies in healthy humans, differential conditioning paradigms combined interoceptive visceral pain with somatic pain (study 1) and aversive tone (study 2) as exteroceptive threats. Conditioned responses to interoceptive threat predictors were enhanced in both studies, consistently involving the insula and cingulate cortex. Interoceptive threats had a greater impact on extinction efficacy, resulting in disruption of ongoing extinction (study 1), and selective resurgence of interoceptive CS-US associations after complete extinction (study 2). In the face of multiple threats, we preferentially learn, store, and remember interoceptive danger signals. As key mediators of nocebo effects, conditioned responses may be particularly relevant to clinical conditions involving disturbed interoception and chronic visceral pain.
Depletion of kinesin motor KIF20A to target cell fate control suppresses medulloblastoma tumour growth
Authors: Qiu Runxiang , Wu Jun , Gudenas Brian , Northcott Paul A. , Wechsler-Reya Robert J. , Lu Qiang 
Published: May 2021

AbstractDuring mammalian brain development, neural progenitor cells proliferate extensively but can ensure the production of correct numbers of various types of mature cells by balancing symmetric proliferative versus asymmetric differentiative cell divisions. This process of cell fate determination may be harnessed for developing cancer therapy. Here, we test this idea by targeting KIF20A, a mitotic kinesin crucial for the control of cell division modes, in a genetic model of medulloblastoma (MB) and human MB cells. Inducible Kif20a knockout in both normal and MB-initiating granule neuron progenitors (GNPs) causes early cell cycle exit and precocious neuronal differentiation without causing cytokinesis failure and suppresses the development of Sonic Hedgehog (SHH)-activated MB. Inducible KIF20A knockdown in human MB cells inhibits proliferation both in cultures and in growing tumors. Our results indicate that targeting the fate specification process of nascent daughter cells presents a novel avenue for developing anti-proliferation treatment for malignant brain tumors.