bluuebunny/crossref_metadata_2025_split huggingface dataset:
Threshold of heteroplasmic truncating MT-ATP6 mutation in reprogramming, Notch hyperactivation and motor neuron metabolism
Authors: Kenvin Sebastian , Torregrosa-Muñumer Ruben , Reidelbach Marco , Pennonen Jana , Turkia Jeremi J , Rannila Erika , Kvist Jouni , Sainio Markus T , Huber Nadine , Herukka Sanna-Kaisa , Haapasalo Annakaisa , Auranen Mari , Trokovic Ras , Sharma Vivek , Ylikallio Emil , Tyynismaa Henna 
Published: October 2021

Abstract
Mutations in mitochondrial DNA encoded subunit of ATP synthase, MT-ATP6, are frequent causes of neurological mitochondrial diseases with a range of phenotypes from Leigh syndrome and NARP to ataxias and neuropathies. Here we investigated the functional consequences of an unusual heteroplasmic truncating mutation m.9154C&gt;T in MT-ATP6, which caused peripheral neuropathy, ataxia and IgA nephropathy. ATP synthase not only generates cellular ATP, but its dimerization is required for mitochondrial cristae formation. Accordingly, the MT-ATP6 truncating mutation impaired the assembly of ATP synthase and disrupted cristae morphology, supporting our molecular dynamics simulations that predicted destabilized a/c subunit subcomplex. Next, we modeled the effects of the truncating mutation using patient-specific induced pluripotent stem cells. Unexpectedly, depending on mutation heteroplasmy level, the truncation showed multiple threshold effects in cellular reprogramming, neurogenesis and in metabolism of mature motor neurons (MN). Interestingly, MN differentiation beyond progenitor stage was impaired by Notch hyperactivation in the MT-ATP6 mutant, but not by rotenone-induced inhibition of mitochondrial respiration, suggesting that altered mitochondrial morphology contributed to Notch hyperactivation. Finally, we also identified a lower mutation threshold for a metabolic shift in mature MN, affecting lactate utilization, which may be relevant for understanding the mechanisms of mitochondrial involvement in peripheral motor neuropathies. These results establish a critical and disease-relevant role for ATP synthase in human cell fate decisions and neuronal metabolism.
Energy, exergy, economic and exergoenvironmental analyses of transcritical CO2 cycle powered by single flash geothermal power plant
Authors: El Haj Assad Mamdouh , Aryanfar Yashar , Javaherian Amirreza , Khosravi Ali , Aghaei Karim , Hosseinzadeh Siamak , Pabon Juan , Mahmoudi SMS 
Published: October 2021

Abstract
The need for energy is increasing worldwide as the population has a continuous trend of increase. The restrictions on energy sources are becoming tougher as the authorities set these developed and developing countries. This leads to looking for other alternative energy sources to replace the conventional energy sources, leading to greenhouse emissions. Environmentally friendly energy sources (renewable energies), for example, geothermal, solar and wind, are viewed as clean and sustainable energy sources. Among these kinds of energy sources, geothermal energy is one of the best options because, like solar and wind energy sources, it does not depend on weather conditions. In this work, a single flash geothermal power plant is used to power a transcritical CO2 power plant is proposed. The energy and exergy analysis of the proposed combined power plant has been performed and the best possible operating mode of the power plant has been discussed. The effects of parameters such as separator pressure, CO2 condenser temperature and CO2 turbine inlet pressure and the pinch point on the energy efficiency, exergy efficiency and output power are determined and discussed. Our results indicate that the highest exergy destruction is in the CO2 vapor generator of 182.4 kW followed by the CO2 turbine of 106 kW, then the CO2 condenser of 82.81 kW and then the CO2 pump 58.76 kW. The lowest exergy destruction rates occur in the single flash geothermal power plant components where the separator has exactly zero exergy destruction rate. The results also show that the combined power plant produces more power and has better efficiencies (first law and second law) than the stand-alone geothermal power plant. Finally, Nelder–Mead simplex method is applied to determine the optimal parameters such as separator pressure, power output and pumps input power and second law efficiency. The results show that the power plant should be operated at a lower pinch temperature to reduce damage to the environment. As the condenser pressure increases, the environmental damage effectiveness coefficient decreases sharply until it reaches the minimum value of 1.2 to 1.7 MPa and then starts to increase. The trend of the impact of sports on environmental improvement is exactly the opposite of the trend of the effectiveness of environmental damage. Therefore, from an environmental point of view, it is recommended to operate the gas turbine at a high inlet pressure.
5 Effects of Virtual Fencing on Cortisol Concentrations and Behavior of Beef Cattle
Authors: Jeffus Jancy , Reuter Ryan R , Wagner Kevin , Goodman Laura , Parker Todd 
Published: October 2021

Abstract
Two experiments were conducted to evaluate the effects of virtual fencing on stress in beef cattle. In both experiments, cattle were contained by either physical, 2-strand electric fencing or by use of a proprietary, GPS-based virtual fencing collar, with no physical interior fencing. In both studies, hair from the tail switch was collected at the end of the experiment, and analyzed for cortisol concentration to measure the accumulated stress experienced by the cattle. Additionally, a subset of cattle were fitted with pedometers to measure behavior responses. In the initial pilot experiment, 55 heifers were grazed for 28 d in either 1 physically-fenced pasture or 1 virtually-fenced pasture. In the second experiment, 59 mature cows and heifers were grazed for 56 d in 1 of 2 physically-fenced or 1 of 2 virtually-fenced pastures (4 pastures total). In the second experiment, blood samples were also collected and NEFA and lactate were quantified in plasma. Only descriptive statistics were summarized for the pilot experiment, which indicated that hair cortisol concentrations were within published ranges for both fence types (0.39 ± 0.32 and 0.37 ± 0.15 pg/mg for physically- or virtually-fenced animals, respectively), and step count and motion index appeared elevated in the first few days of adaptation to virtual fence. The second experiment was analyzed with analysis of variance as a completely random design with pasture as the experimental unit. No effect of fence type was found for behavior (number of steps, standing time, lying bouts, or motion index; P &gt; 0.16). Further, no difference was observed in cortisol, lactate nor NEFA (P &gt; 0.14) concentrations due to fence type. None of this data suggests that virtual fencing is different from physical fencing in the effects on cattle stress and behavior, indicating further research and development of virtual fencing technology are warranted.
15 Differentially Expressed Long Non-coding RNA Associated with Gastrointestinal Nematode Infection in Divergent Immune Response Sheep
Authors: Muniz Maria Malane M , Boareki Mohammed , Dixon Samantha , Peregrine Andrew S , Menzies Paula , Karrow Niel , Cánovas Angela 
Published: October 2021

Abstract
Gastrointestinal nematode infection is one of the major production problems for sheep producers worldwide due its high incidence, morbidity, and mortality in affected flocks. The study of long non-coding RNA (lncRNA) in liver tissue of high (HIR) and low immune responder (LIR) sheep to GINs using RNA-Sequencing technology may provide a better understanding of the gene regulation mechanism associated with the host response to the infection. The aim of this study was to identify differentially expressed (DE) lncRNA between HIR and LIR natural infested sheep and control group. Liver tissue samples from the 13 divergent animals (out of a population of 211) based on their immunoglobulin G levels after vaccination using Hen Egg White (HEW) Lysozyme, and immature abomasum worm counts [HIR (&gt; 4000) (n = 5), LIR (&lt; 1500) (n=5) and control (no parasite challenge) (n=4) groups] were used to perform transcriptomic analysis using RNA-Sequencing. The “Large Gap read mapping “and “Transcript Discovery” tools from CLC Genomics Workbench 20.0.4 (CLC Bio, Aarhus, Denmark), were used to map reads to a reference genome (Oar_rambouillet_v1.0) and transcript discovery, respectively. The FEELnc software was used to identify, from predicted transcript model, potential lncRNAs and classify those transcripts into intro putative lncRNAs and protein coding RNAs. As preliminary results, 8 and 48 DE lncRNAs for HIR and LIR compared to control group were identified, respectively using an adjusted p-value False Discovery Rate (FDR) &lt; 0.05 and Fold change (FC) abs &gt; 2. Functional analyses using the list of DE lncRNAs identified metabolic pathways related to immune function. In depth analysis will help to better understand the physiological mechanisms of resilience of high immune sheep.
45 Partitioning SNP Heritability in Related Individuals
Authors: Jiang Jicai , Ma Li , O’Connell Jeffrey 
Published: October 2021

Abstract
Partitioning SNP heritability by many functional annotations has been a successful tool for understanding the genetic architecture of complex traits in human genetic studies. Similar analyses are being extended to animal research, as (imputed) whole-genome sequence data of many individuals and various functional annotations have become available in livestock animals. Though many approaches have been developed for heritability partition (e.g., LDSC and HE-reg), they are mostly based on approximations tailored to human populations and few can produce statistically efficient estimates for animal genomic studies where individuals are often related. To tackle this issue, we present a stochastic MINQUE (Minimum Norm Quadratic Unbiased Estimation) approach for partitioning SNP heritability, which we refer to as MPH. We provide a theoretical analysis comparing LDSC and HE-reg with REML and MPH and demonstrate what LDSC and HE-reg (and similar methods) take advantage of in their approximations: sparse relationships between individuals and relatively weak linkage disequilibrium. We also show that our method is mathematically equivalent to the MC-REML approach implemented in BOLT. MPH has three key features. First, it is comparable to genomic REML in terms of accuracy, while being at least one order of magnitude faster than GCTA and BOLT and using only ~1/4 of memory as much as GCTA, when applied to sequence data and many variance components (or functional annotation categories). Second, it can do weighted analyses if residual variances are unequal (such as DYD). Third, it works for many overlapping functional annotations. Using simulations based on a human pedigree and a dairy cattle pedigree, we illustrate the benefits of our method for partitioning SNP heritability in pedigree-based studies. We also demonstrate that it is feasible to efficiently partition SNP heritability for animal genomes with strong, long-span LD. MPH is freely available at https://jiang18.github.io/mph.
62 Feeding Chicory-plantain Silage and Se-yeast to Lactating Ewes Subjected to Intramammary Infection: Effect on the Immune System
Authors: Ford Hunter , Bionaz Massimo , Ates Serkan , Klopfenstein Joe , Vanegas Jorge , Busato Sebastiano , Hasan Daniella , Park Shelby , Pearce Carolyn , Quinones Tyler , Ness Kate , Torres Mariangel Aleman 
Published: October 2021

Abstract
The objective was to test the effect on the immune status by feeding a combination of chicory-plantain and Se-yeast in lactating ewes subjected to intramammary infection (IMI) with 2×107CFU of Strep uberis in both glands. For the purpose we enrolled 28 Polypay lactating sheep from a prior study where they were randomly allocated to receive chicory (CS) or grass (GC) silage and either 3.6 mg of Se/day as Se-yeast (DiaMune, Diamond V) (Y) or isoenergetic-isonitrogenous alfalfa meal (C) for 2 months. For the present study, ewes were kept on the original dietary regiment except the CT group received a chicory-plantain silage(50% each). Blood was collected prior to and for 10 days after IMI for a complete blood count(VetScan HM5), leukocytes migration, and rectal temperature (RT). Data were analyzed using GLIMMIX (SAS)with time, silage type, and Se and their interactions as the fixed effects and ewe as random effect with significance declared at P ≤ 0.05. RT was lower in chicory vs. grass before IMI and Se limited the RT increase after IMI. Total WBC levels tended (P = 0.06) to increase in animals fed with Se after IMI, which was driven by a larger number of lymphocytes. Hematocrit, red blood cells, and hemoglobin were strongly decreased by IMI. The mean cell volume was overall larger in ewes fed with chicory-plantain silage while mean cell hemoglobin was larger in animal fed with grass silage. The platelet distribution width was affected by silage*Se interaction due to a positive effect by Se in grass-fed but negative in chicory-plantain-fed ewes. Migration of neutrophils was larger in animals fed with chicory-plantain before IMI but similar between groups after IMI. Overall, these findings indicate that Se supplementation can increase lymphocytes with no effect on neutrophils while activity of neutrophils is positively affected by feeding chicory-plantain silage.
63 Effect of Chicory Silage and Se-yeast on Peripartum Ewes: Performance Data
Authors: Ford Hunter , Bionaz Massimo , Ates Serkan , Klopfenstein Joe , Hasan Daniella , Hernandez Gracia Puerto , VanDerZanden Julia , Ibarra Magdalena , Dougherty Caitlynn , Heney Samantha 
Published: October 2021

Abstract
The objective of this study was to test the possibility of improving the performance of peripartum ewes by feeding a combination of chicory silage and Se-yeast. Approximately four weeks prior to lambing, 36 pregnant Polypay ewes were moved into single pens and randomly assigned to one of four groups to be fed either grass or chicory silage and supplemented or not with 3.6 mg Se/day (DiaMune, Diamond V). Daily dry matter intake (DMI), water intake, body weight gain of lambs and body weight changes of the ewes, milk yield using a weigh-suckle-weigh system, and milk components were recorded. Data were analyzed using GLIMMIX (SAS v9) with time, silage, and Se interaction as fixed effects and ewe as random effect. Significance was declared with P ≤ 0.05. The DMI was lower in ewes fed chicory compared to grass silage with no effect of Se. Water intake was greater in ewes that were fed chicory silage than grass silage but lower in those supplemented with Se. Milk production of ewes and BW gain of the lambs were not affected by the treatments, but the gain efficiency (G:F) of lambs was larger in animal fed chicory vs. grass silage. Few differences were observed in milk components between the treatments. Protein content and SNF tended to be reduced by feeding Se (P = 0.08) while feeding Se increased C18:0 and preformed fatty acids in milk. Feeding chicory+Se tended to increase percentage of de novo-derived fatty acids in milk compared to feeding grass+Se. Somatic cell count (SCC) was not affected by the treatments. Overall, our data indicated that performance of the animals as well as milk components were not affected by feeding chicory and/or Se despite a lower feed intake when chicory was fed. Higher feed efficiency by using chicory is suggested by the DMI/gain of lambs.
71 The Use of Medium Chain Fatty Acids to Reduce Porcine Reproductive Respiratory Syndrome Virus Replication in MARC-145 Cells
Authors: Crowder Stacie , Pogranichniy Roman , DeRodas Brenda , Peter Karnezos , Radcliffe J S S 
Published: October 2021

Abstract
Porcine reproductive respiratory syndrome virus (PPRSV) costs the U.S. Swine Industry an estimated $664 million annually. The objective of this study was to evaluate the effect of medium chain fatty acids (MCFA) on PRRSV replication in M-145 cells. Two experiments were conducted to 1) evaluate the use of individual MCFAs (C6, C8, C10), and 2) evaluate MCFA combinations (C8/C10, C10/C12, C8/C10/C12) on viral replication of PRRSV. Experiment one used individual MCFAs at 7 concentrations from 1-1000µg/ml compared to a control. Experiment two used MCFA combinations at 6 different concentrations from 50–500µg/ml compared to a control. North American Type II P-129 PRRSV and European Type I Lelystad PRRSV strains were used. Viral replication was determined using FITC labeled IgG anti-PRRSV monoclonal antibody and TCID50 was calculated for each treatment concentration using 5 wells per treatment at each virus concentration with plates run in triplicate. Data were analyzed using Proc Mixed procedures of SAS. In experiment 1, C6 had no effect on replication of PRRSV in M145 cells. C8 induced a 3.02 and 2.02 log reduction in TCID50 for type I and type II virus strains, respectively (P &lt; 0.01) at 1000µg/ml. C10 induced a 2.85 and 3.23 log reduction in TCID50 for type I and type II virus strains, respectively (P &lt; 0.01) at 300µg/ml. In experiment 2, C8/C10 induced a 1.9 and 2.2 log reduction in TCID50 for type I and type II virus strains, respectively (P &lt; 0.01) at 200µg/ml. C10/C12 resulted in a 3.37 and 2.14 log reduction in TCID50 at 200µg/ml for type I and type II virus strains, respectively (P &lt; 0.01). C8/C10/C12 resulted in a 1.34 and 1.56 log reduction in TCID50 at 200µg/ml for type I and type II virus strains (P &lt; 0.01), respectively.
70 Occurrence of Mycotoxins in 2020 US Corn Silage and Dairy Total Mixed Rations
Authors: Gott Paige N , Schwandt Erin , Ramirez Shelby M , Hofstetter Ursula , Murugesan Raj 
Published: October 2021

Abstract
Mycotoxins are fungal metabolites which have been identified in many feed ingredients and ruminants have an increased risk of exposure due to the complex nature of their diets. Despite varying degrees of natural detoxification in the rumen, cattle health, productivity, and reproduction can be compromised by mycotoxins. This study investigated mycotoxin occurrence and contamination levels in 2020 U.S. corn silage and dairy total mixed ration (TMR) samples. Samples were screened via LC-MS/MS for six major mycotoxin groups including: aflatoxins, type A trichothecenes (A-Trich), type B trichothecenes (B-Trich), fumonisins (FUM), zearalenone (ZEN), and ochratoxin A. Information was collected with each submission including state of origin and whether or not clinical health or performance concerns were present. Contamination levels were analyzed using the GLIMMIX procedure (SAS 9.4, Cary, NC) to investigate the interaction of clinical concern (yes/no) and harvest year. No interaction was statistically significant for each of the toxin groups, so main effects of harvest year are presented in Table 1. Type B trichothecenes have been identified most frequently in 2020 corn silage. Although not often detected, A-Trich occurrence has increased ten percentage points from 2019 crop year. Among 236 TMR samples analyzed, B-Trich have been detected in a high percentage of samples. Mean contamination levels (parts per billion, ppb) are presented on a dry matter basis and within each data set were similar in 2020 compared to 2019 for respective toxin groups. Preliminary survey results indicate B-Trich occur frequently in both U.S. corn silage and TMR samples. Despite less frequent detection, other mycotoxin groups do occur including ZEN, FUM, and A-Trich, so the potential risk from toxin interactions due to co-contamination should be considered.
72 A One-health Perspective on S. aureus Induced Mastitis
Authors: Zhao Xin , Ibeagha-Awemu Eveline M , Lacasse Pierre 
Published: October 2021

Abstract
Bovine mastitis, commonly caused by bacteria, is one of the most devastating diseases for the dairy industry worldwide, with high economic, social, and public health impacts. Among the pathogenic bacteria, Staphylococcus aureus is one of the leading pathogens in most countries. Success rate of antibiotic treatment is low such that effective control of S. aureus induced mastitis is currently only possible through prevention of new infections and culling of infected animals. The infection is usually subclinical, causing elevated somatic cell counts but no detectable changes in milk or the udder. S. aureus persists in mammary glands, teat canals, and teat lesions of infected cows and is transmitted to other cows during milking. The direct evidence of cross-species transmission of S. aureus is still scarce. Nevertheless, accumulating indirect evidence supports the zoonotic potential for S. aureus from bovine mastitis to humans. In addition to direct contact, aerosol exposures may also be an important mechanism for the transmission. While animal-adapted livestock-associated methicillin-resistant S. aureus has been known for many years, most S. aureus isolated from mastitic milk in Canada are methicillin-sensitive. S. aureus induced mastitis is a common reason for therapeutic and/or prophylactic use of antibiotics on dairy farms. Occurrence of multidrug resistant, especially methicillin-resistant, S. aureus has been a major public health concern. In this talk, we will address the pathogenesis and strain spectrum of S. aureus induced bovine mastitis, review existing evidence for inter-species transmission, and discuss possible transmission of methicillin-resistance determinants. This talk aims to emphasize the need for the one-health approach for prevention and treatment of S. aureus induced mastitis, in order to provide safe and nutritious milk and milk products to consumers.
75 Can Regionalized Livestock Production and Meat Packing Meet the Food Needs of Today’s Consumers?
Authors: Apple Jason 
Published: October 2021

Abstract
Meat shortages in many of the largest retail chains during the early months of the COVID-19 pandemic affected millions of U.S. consumers. In addition, wait times for custom slaughter of meat animals increased from days to weeks to upwards of 14 mon. Interruptions in livestock slaughter and meat supplies have renewed the emphasis on medium, small, and very small meat slaughters/processors. Numerous states are investing in slaughter/processing plant construction, renovation of existing plants, and establishing or reestablishing state inspection programs. It is conceivable that this reinvestment may alleviate some of the meat supply limitations; yet, there are a number of factors these plants need to address for economic sustainability, including (but are not limited to): consistency of local and regional livestock supply; availability of trained, experienced workforce; plant holding pens and slaughter floor design; pre-slaughter animal welfare training and compliance; development and implementation of food safety programs; fresh and frozen storage capacities; local and regional marketing channels and modes of distribution; and by-products markets and offal disposal. Regardless of plant size, the ultimate goal of all meat packers/processors is the production of consistent, readily available and affordable, high-quality meat and meat products; however, the traditional driving forces of price and taste are being slowly supplanted by consumers’ concerns about production practices and animal management, perceived nutritional benefits, animal welfare concerns, food locality, and conveniences. This presentation will attempt to amalgamate the challenges facing medium, small, and very small meat processors with consumers’ preferences in relation to the sustainability of these revitalized segments of the livestock and meats industry.