bluuebunny/crossref_metadata_2025_split huggingface dataset:
Results of a web-based questionnaire: A gender-based study of migraine with and without aura and possible differences in pain perception and drug effectiveness
Authors: Drescher Johannes , Amann Tina Katharina , Gaul Charly , Kropp Peter , Siebenhaar Yannic , Scheidt Jörg 
Published: December 2021

Background: The aim of this work is to analyze reports of migraine attacks collected online in the citizen science project CLUE with respect to gender- and migraine type-specific differences in drug effectiveness and pain perception. Citizen science project data collection opens the possibility to examine these differences based on a large number of individual attacks instead of a simple survey of patients. Methods: One thousand three hundred and ninety four participants reported 47,274 migraine attacks via an online platform and smartphone apps. The reports contained information on the acute medications taken, the evaluation of their effect, and information on pain parameters such as pain intensity, origin, and localization. Chi-square tests were used to investigate whether the effect of acute medications and pain parameters differed when collated by gender and migraine type (migraine with and without aura). Results: Our participants rated the effectiveness of triptans as significantly better than that of ibuprofen. For triptans, significant differences in effectiveness were found when migraine types were distinguished, but no difference was found between genders. For ibuprofen, there were no differences between migraine types but significant differences between gender groups. Examination of pain parameters reveals differences between groups in pain intensity, pain origin, and pain location. The differences are statistically significant, but the effects are small. Conclusions: Despite some methodological limitations, web-based data collection is able to support findings from clinical trials in a real-world setting. Due to the high numbers of participants included and attacks reported, even small differences in medication efficacy and pain parameters between the groups considered can be demonstrated to be statistically significant.
Full-length transcriptomic identification of R2R3-MYB family genes related to secondary cell wall development in Cunninghamia lanceolata (Chinese fir)
Authors: Zhuang Hebi , Chong Sun-Li , Priyanka Borah , Han Xiao , Lin Erpei , Tong Zaikang , Huang Huahong 
Published: December 2021

Abstract
Background
R2R3-MYB is a class of transcription factor crucial in regulating secondary cell wall development during wood formation. The regulation of wood formation in gymnosperm has been understudied due to its large genome size. Using Single-Molecule Real-Time sequencing, we obtained full-length transcriptomic libraries from the developmental stem of Cunninghamia lanceolata, a perennial conifer known as Chinese fir. The R2R3-MYB of C. lanceolata (hereafter named as ClMYB) associated with secondary wall development were identified based on phylogenetic analysis, expression studies and functional study on transgenic line.

Results
The evolutionary relationship of 52 ClMYBs with those from Arabidopsis thaliana, Eucalyptus grandis, Populus trichocarpa, Oryza sativa, two gymnosperm species, Pinus taeda, and Picea glauca were established by neighbour-joining phylogenetic analysis. A large number of ClMYBs resided in the woody-expanded subgroups that predominated with the members from woody dicots. In contrast, the woody-preferential subgroup strictly carrying the members of woody dicots contained only one candidate. The results suggest that the woody-expanded subgroup emerges before the gymnosperm/angiosperm split, while most of the woody-preferential subgroups are likely lineage-specific to woody dicots. Nine candidates shared the same subgroups with the A. thaliana orthologs, with known function in regulating secondary wall development. Gene expression analysis inferred that ClMYB1/2/3/4/5/26/27/49/51 might participate in secondary wall development, among which ClMYB1/2/5/26/27/49 were significantly upregulated in the highly lignified compression wood region, reinforcing their regulatory role associated with secondary wall development. ClMYB1 was experimentally proven a transcriptional activator that localised in the nucleus. The overexpression of ClMYB1 in Nicotiana benthamiana resulted in an increased lignin deposition in the stems. The members of subgroup S4, ClMYB3/4/5 shared the ERF-associated amphiphilic repression motif with AtMYB4, which is known to repress the metabolism of phenylpropanoid derived compounds. They also carried a core motif specific to gymnosperm lineage, suggesting divergence of the regulatory process compared to the angiosperms.

Conclusions
This work will enrich the collection of full-length gymnosperm-specific R2R3-MYBs related to stem development and contribute to understanding their evolutionary relationship with angiosperm species.
High expression of eIF4E is associated with tumor macrophage infiltration and leads to poor prognosis in breast cancer
Authors: Li Fan , Sun Huizhi , Li Yue , Bai Xiaoyu , Dong Xueyi , Zhao Nan , Meng Jie , Sun Baocun , Zhang Danfang 
Published: December 2021

Abstract
Background
The expression and activation of eukaryotic translation initiation factor 4E (eIF4E) is associated with cell transformation and tumor initiation, but the functional role and the mechanism whereby it drives immune cell infiltration in breast cancer (BRCA) remain uncertain.

Methods
Oncomine, Timer and UALCAN were used to analyze the expression of eIF4E in various cancers. PrognoScan, Kaplan–Meier plotter, and GEPIA were utilized to analyze the prognostic value of eIF4E in select cancers. In vitro cell experiments were used to verify the role of eIF4E in promoting the progression of BRCA. ImmuCellAI and TIMER database were used to explore the relationship between eIF4E and tumor infiltrating immune cells. The expression of a macrophage marker (CD68+) and an M2-type marker (CD163+) was evaluated using immunohistochemistry in 50 invasive BRCA samples on tissue microarrays. The Human Protein Atlas (HPA) database was used to show the expression of eIF4E and related immune markers. LinkedOmics and NetworkAnalyst were used to build the signaling network.

Results
Through multiple dataset mining, we found that the expression of eIF4E in BRCA was higher than that in normal tissues, and patients with increased eIF4E expression had poorer survival and a higher cumulative recurrence rate in BRCA. At the cellular level, BRCA cell migration and invasion were significantly inhibited after eIF4E expression was inhibited by siRNA. Immune infiltration analysis showed that the eIF4E expression level was significantly associated with the tumor purity and immune infiltration levels of different immune cells in BRCA. The results from immunohistochemical (IHC) staining further proved that the expression of CD68+ and CD163+ were significantly increased and correlated with poor prognosis in BRCA patients (P < 0.05). Finally, interaction network and functional enrichment analysis revealed that eIF4E was mainly involved in tumor-related pathways, including the cell adhesion molecule pathway and the JAK-STAT signaling pathway.

Conclusions
Our study has demonstrated that eIF4E expression has prognostic value for BRCA patients. eIF4E may act as an essential regulator of tumor macrophage infiltration and may participate in macrophage M2 polarization.
Maternal Anxiety and Infants Birthweight and Length of Gestation. A sibling design
Authors: Bekkhus Mona , Lee Yunsung , Brandlistuen Ragnhild Eek , Samuelsen Sven Ove , Magnus Per 
Published: December 2021

Abstract
Background
The overall aim of this study is to examine the effect of prenatal maternal anxiety on birthweight and gestational age, controlling for shared family confounding using a sibling comparison design.

Methods
The data on 77,970 mothers and their 91,165 children from the population-based Mother, Father and Child Cohort Study and data on 12,480 pairs of siblings were used in this study. The mothers filled out questionnaires for each unique pregnancy, at 17th and 30th week in pregnancy. Gestational age and birth weight was extracted from the Medical Birth Registry of Norway (MBRN). Associations between prenatal maternal anxiety (measured across the 17th and 30th weeks) and birth outcomes (birthweight and gestational age) were examined using linear regression with adjustment for shared-family confounding in a sibling comparison design.

Results
In the population level analysis the maternal anxiety score during pregnancy was inversely associated with new-born’s birthweight (Beta = -63.8 95% CI: -92.6, -35.0) and gestational age (Beta = -1.52, 95% CI: -2.15, -0.89) after adjustment for several covariates. The association of the maternal anxiety score with birthweight was no longer significant, but remained for maternal anxiety at 30th week with gestational age (Beta = -1.11, 95% CI: -1.82, -0.4) after further adjusting for the shared-family confounding in the sibling comparison design.

Conclusion
No association was found for maternal prenatal anxiety with birth weight after multiple covariates and family environment were controlled. However, there was an association between prenatal maternal anxiety at 30th week only with gestational age, suggesting a timing effect for maternal anxiety in third trimester.
The association between sexual function and body image among postmenopausal women: a cross-sectional study
Authors: Nazarpour Soheila , Simbar Masoumeh , Khorrami Mobina , Jafari Torkamani Zahra , Saghafi Reyhaneh , Alavi-Majd Hamid 
Published: December 2021

Abstract
Background
Postmenopause physiological changes may alter body image (BI) during the postmenopausal period of life. Body image dissatisfaction may have negative effects on the sexual function of women. The present study aims to assess the relationship between body image and sexual function during the postmenopausal period.

Methods
This cross-sectional study was performed on 231 postmenopausal women who were menopausal during the recent 5 years, aged > 40 years old, and referring to health centers in Tehran-Iran. The participants were recruited using a multi-stage sampling method. Data were collected using 3 questionnaires including socio-demographic, Fisher’s Body Image, and female sexual function index (FSFI). Data were analyzed using SPSS 24 and multiple regression, Mann–Whitey, Pearson, and Spearman correlation tests.

Results
Two hundred thirty-one postmenopausal women aged 52.53 ± 5.32 (mean ± SD) years old participated in the study. The mean and standard deviation of FSFI and BI of the women were respectively 19.59 ± 11.11(ranges 1.2–36) and 155.43 ± 37.09 (ranges from 46 to 230). According to FSFI, 62.8% of female sexual dysfunction (FSD) was reported. There were significant correlations between scores of total and all domains of FSFI with scores of total and all dimensions of BI. There was also a positive correlation between education and family income of the women and a negative correlation between age of husband and duration of menopause with their sexual function. Significant relationships were also shown between the women's and the husbands' jobs with the total score of women's sexual function. According to the multiple linear regression model, BI was a significant predictor of sexual function in postmenopausal women.

Conclusion
Body image is effective on the sexual function of postmenopausal women. Therefore, body image is necessary to be considered in future postmenopausal health promotion programs.
Next-generation in vitro blood–brain barrier models: benchmarking and improving model accuracy
Authors: Linville Raleigh M. , Searson Peter C. 
Published: December 2021

AbstractWith the limitations associated with post-mortem tissue and animal models, In vitro BBB models enable precise control of independent variables and microenvironmental cues, and hence play an important role in studying the BBB. Advances in stem cell technology and tissue engineering provide the tools to create next-generation in vitro BBB models with spatial organization of different cell types in 3D microenvironments that more closely match the human brain. These models will be capable of assessing the physiological and pathological responses to different perturbations relevant to health and disease. Here, we review the factors that determine the accuracy of in vitro BBB models, and describe how these factors will guide the development of next-generation models. Improving the accuracy of cell sources and microenvironmental cues will enable in vitro BBB models with improved accuracy and specificity to study processes and phenomena associated with zonation, brain region, age, sex, ethnicity, and disease state.
Analytical setup margin for spinal stereotactic body radiotherapy based on measured errors
Authors: Copeland Audrey , Barron Addie , Fontenot Jonas 
Published: December 2021

Abstract
Background
No consensus currently exists about the correct margin size to use for spinal SBRT. Margins have been proposed to account for various errors individually, but not with all errors combined to result in a single margin value. The purpose of this work was to determine a setup margin for five-fraction spinal SBRT based on known errors during radiotherapy to achieve at least 90% coverage of the clinical target volume with the prescription dose for at least 90% of patients and not exceed a 30 Gy point dose or 23 Gy to 10% of the spinal cord subvolume.

Methods
The random and systematic error components of intrafraction motion, residual setup error, and end-to-end system accuracy were measured. The patient’s surface displacement was measured to quantify intrafraction motion, the residual setup error was quantified by re-registering accepted daily cone beam computed tomography setup images, and the displacement between measured and planned dose profiles in a phantom quantified the end-to-end system accuracy. These errors and parameters were used to identify the minimum acceptable margin size. The margin recommendation was validated by assessing dose delivery across 140 simulated patient plans suffering from various random shifts representative of the measured errors.

Results
The errors were quantified in three dimensions and the analytical margin generated was 2.4 mm. With this margin applied in the superior/inferior direction only, at least 90% of the CTV was covered with the prescription dose for 96% of the 140 patients simulated with minimal negative effect on the spinal cord dose levels.

Conclusions
The findings of this work support that a 2.4 mm margin applied in the superior/inferior direction can achieve at least 90% coverage of the CTV for at least 90% of dual-arc volumetric modulated arc therapy spinal SBRT patients in the presence of errors when immobilized with vacuum bags.
Studies on the effects of hypothermia combined with hypoxia on rat skeletal muscle and lipid metabolism based on AMPK/PGC1α pathway
Authors: Ruixia Zhang , chuanchuan Liu , Lu Guan , Shuang Ma , Qiang Zhu , Xiaofang Tian , Yinggui Ba 
Published: December 2021

Abstract
Aim
To explore the effects of hypothermia and hypoxia on rat skeletal muscle and lipid metabolism.

Method
Forty male rats were randomly divided into blank group, low-temperature group, hypoxia group, and hypothermia combined with hypoxia group. The body weight of the rats was monitored. The changes of Irisin were detected by ELISA, and LDL, HDL, TC, and TG levels in serum were detected by blood biochemistry. Western blot was used to detect the changes of lipid metabolism-related proteins. CCK8 was used to verify the effect of AMPK/PGC1α on the proliferation of rat skeletal muscle cells.

Result
In the case of cold stimulation and hypoxia, the weight of the rats decreased significantly, and the levels of LDL, HDL, TC, and TG in the serum were abnormal. The activity of fatty acid metabolism factors Irisin, UCP-1, and FABP4 is down-regulated by hypothermia and hypoxia. The activity of fat metabolism-related enzymes, ATGL, HSL, and MGL increased under hypothermia and low oxygen conditions. Hypothermia and hypoxia affected the morphology of skeletal muscle, and AMPK/PGC-1α can regulate the proliferation of skeletal muscle cells.

Conclusion
Hypothermia and hypoxia can reduce the body weight of rats, and affect the structure of skeletal muscle to promote lipid metabolism through AMPK/PGC-1α signaling pathway.
Lahar risk assessment from source identification to potential impact analysis: the case of Vulcano Island, Italy
Authors: Gattuso Alessandro , Bonadonna Costanza , Frischknecht Corine , Cuomo Sabatino , Baumann Valérie , Pistolesi Marco , Biass Sebastien , Arrowsmith J. Ramon , Moscariello Mariagiovanna , Rosi Mauro 
Published: December 2021

AbstractLahars are rapid flows composed of water and volcaniclastic sediments, which have the potential to impact residential buildings and critical infrastructure as well as to disrupt critical services, especially in the absence of hazard-based land-use planning. Their destructive power is mostly associated with their velocity (related to internal flow properties and topographic interactions) and to their ability to bury buildings and structures (due to deposit thickness). The distance reached by lahars depends on their volume, on sediments/water ratio, as well as on the geometrical properties of the topography where they propagate. Here we present the assessment of risk associated with lahar using Vulcano island (Italy) as a case study. First, we estimated an initial lahar source volume considering the remobilisation by intense rain events of the tephra fallout on the slopes of the La Fossa cone (the active system on the island), where the tephra fallout is associated with the most likely scenario (e.g. long-lasting Vulcanian cycle). Second, we modelled and identified the potential syn-eruptive lahar impact areas on the northern sector of Vulcano, where residential and touristic facilities are located. We tested a range of parameters (e.g., entrainment capability, consolidation of tephra fallout deposit, friction angle) that can influence lahar propagation output both in terms of intensity of the event and extent of the inundation area. Finally, exposure and vulnerability surveys were carried out in order to compile exposure and risk maps for lahar-flow front velocity (semi-quantitative indicator-based risk assessment) and final lahar-deposit thickness (qualitative exposure-based risk assessment). Main outcomes show that the syn-eruptive lahar scenario with medium entrainment capability produces the highest impact associated with building burial by the final lahar deposit. Nonetheless, the syn-eruptive lahar scenario with low entrainment capacity is associated with higher runout and results in the highest impact associated with lahar-flow velocities. Based on our simulations, two critical infrastructures (telecommunication and power plant), as well as the main road crossing the island are exposed to potential lahar impacts (either due to lahar-flow velocity or lahar-deposit thickness or both). These results show that a risk-based spatial planning of the island could represent a valuable strategy to reduce the volcanic risk in the long term.
Living systematic reviews in rehabilitation science can improve evidence-based healthcare
Authors: Elbers S. , Wittink H. , Kaiser U. , Kleijnen J. , Pool J. , Köke A. , Smeets R. 
Published: December 2021

AbstractAlthough systematic reviews are considered as central components in evidence-based practice, they currently face an important challenge to keep up with the exponential publication rate of clinical trials. After initial publication, only a minority of the systematic reviews are updated, and it often takes multiple years before these results become accessible. Consequently, many systematic reviews are not up to date, thereby increasing the time-gap between research findings and clinical practice. A potential solution is offered by a living systematic reviews approach. These types of studies are characterized by a workflow of continuous updates which decreases the time it takes to disseminate new findings. Although living systematic reviews are specifically designed to continuously synthesize new evidence in rapidly emerging topics, they have also considerable potential in slower developing domains, such as rehabilitation science. In this commentary, we outline the rationale and required steps to transition a regular systematic review into a living systematic review. We also propose a workflow that is designed for rehabilitation science.
Modulation of retinoid-X-receptors differentially regulates expression of apolipoprotein genes apoc1 and apoeb by zebrafish microglia
Authors: Thiel Whitney A. , Esposito Emma J. , Findley Anna P. , Blume Zachary I. , Mitchell Diana M. 
Published: December 2021

ABSTRACT
Transcriptome analyses performed in both human and zebrafish indicate strong expression of Apoe and Apoc1 by microglia. Apoe expression by microglia is well appreciated, but Apoc1 expression has not been well-examined. PPAR/RXR and LXR/RXR receptors appear to regulate expression of the apolipoprotein gene cluster in macrophages, but a similar role in microglia in vivo has not been studied. Here, we characterized microglial expression of apoc1 in the zebrafish central nervous system (CNS) in situ and demonstrate that in the CNS, apoc1 expression is unique to microglia. We then examined the effects of PPAR/RXR and LXR/RXR modulation on microglial expression of apoc1 and apoeb during early CNS development using a pharmacological approach. Changes in apoc1 and apoeb transcripts in response to pharmacological modulation were quantified by RT-qPCR in whole heads, and in individual microglia using hybridization chain reaction (HCR) in situ hybridization. We found that expression of apoc1 and apoeb by microglia were differentially regulated by LXR/RXR and PPAR/RXR modulating compounds, respectively, during development. Our results also suggest RXR receptors could be involved in endogenous induction of apoc1 expression by microglia. Collectively, our work supports the use of zebrafish to better understand regulation and function of these apolipoproteins in the CNS.
Ο ναός της Παναγίας Οδηγήτριας (;) στον Αρχάγγελο Πρεβέζης (1785) Σύντομη περιγραφή και αποτίμηση
Authors: Καραλής Ηλίας , Κουτρόπουλος Γρηγόριος Χ. 
Published: December 2021

Η εργασία των Καραλή-Κουτρόπουλου, αναφέρεται σε ερειπωμένο ναό του 18ου αιώνα, που βρίσκεται στο χωριό Αρχάγγελος Πρέβεζας. Μολονότι πρόκειται για ιδιαίτερα αξιόλογο μνημείο, ο ναός μέχρι σήμερα δεν έχει αποτελέσει το αντικείμενο ειδικής μελέτης. Σκοπός του παρόντος άρθρου είναι διττός, αφενός να αποτυπώσει και να τεκμηριώσει όσα περισσότερα αρχικά στοιχεία σώζονται ακόμη στο μνημείο λαμβάνοντας υπόψιν τις εγγενείς δυσκολίες μελέτης του, αφετέρου να επεξεργαστεί αυτά τα στοιχεία και να προβούν σε συμπερασματικές προτάσεις, καθώς ο ναός παρουσιάζει επιστημονικό ενδιαφέρον για την Ιστορία της Αρχιτεκτονικής και της Τέχνης.
Η έκθεση του ταγματάρχη Δημητρίου Ν. Δούλη για τη μάχη της Νικοπόλεως, 20.10.1912
Authors: Καράμπελας Νίκος Δ. 
Published: December 2021

Παρουσίαση της εκθέσεως του Δημητρίου Ν. Δούλη για τη μάχη της Νικοπόλεως
O αρχαιολογικός χώρος της Νικόπολης μετά την απελευθέρωση της Πρέβεζας (1913-1967)
Authors: Κωνσταντάκη Αθηνά Ι. 
Published: December 2021

Παρουσίαση του αρχαιολογικού έργου της Νικόπολης από την απελευθέρωση της πόλης μεχρι και τη σύγχρονη εποχή και η πολυεπίπεδη λειτουργία του αρχαιολογικού της χώρου.