bluuebunny/crossref_metadata_2025_split huggingface dataset:
MiR-30a-5p Downregulation Induces Neuronal Autophagy by Activating AMPK After 2856MHz Microwave Radiation
Authors: Hao Yanhui , Li Wenchao , Wang Hui , Zhang Jing , Wang Haoyu , Dong Ji , Yao Binwei , Xu Xinping , Zhao Li , Peng Ruiyun 
Published: November 2021

Abstract
Background With the development of science and technology, microwaves are being widely used. More and more attention has been paid to the potential health hazards of microwave exposure. The regulation of miR-30a-5p (miR-30a) on autophagy is involved in the pathophysiological process of many diseases. Our previous study found that 30 mW/cm2 microwave radiation could reduce miR-30a expression and activate neuronal autophagy in rat hippocampus. However, the roles played by miR-30a in microwave-induced neuronal autophagy and related mechanisms remain largely unexplored. Results In the present study, we established neuronal damage models by exposing rat hippocampal neurons and rat adrenal pheochromocytoma (PC12) cell-derived neuron-like cells to 30 mW/cm2 microwave, which resulted in miR-30a downregulation and autophagy activation in vivo and in vitro. Bioinformatics analysis was conducted, and Beclin1, Prkaa2, Irs1, Pik3r2, Rras2, Ddit4, Gabarapl2 and autophagy-related gene 12 (Atg12) were identified as potential downstream target genes of miR-30a involved in regulating autophagy. Based on our previous findings that microwave radiation can cause a neuronal energy metabolism disorder, Prkaa2, encoding adenosine 5’-monophosphate-activated protein kinase α2 (AMPKα2, an important catalytic subunit of energy sensor AMPK), was selected for further analysis. Dual-luciferase reporter assay results showed that Prkaa2 is a downstream target gene of miR-30a. Microwave radiation increased the expression and phosphorylation (Thr172) of AMPKα both in vivo and in vitro. Moreover, the transduction of cells with miR-30a mimics suppressed AMPKα2 expression, inhibited AMPKα (Thr172) phosphorylation and reduced autophagy flux in neuron-like cells. Importantly, miR-30a mimics abolished microwave-activated autophagy and inhibited microwave-induced AMPKα (Thr172) phosphorylation. Conclusions AMPKα2 was a newly founded downstream gene of miR-30a involved in autophagy regulation, and miR-30a downregulation after microwave radiation could promote neuronal autophagy by increasing AMPKα2 expression and activating AMPK signaling.
Effect of Tourniquet use on Blood Loss, Pain, Functional Recovery, and Complications in Robot-assisted Total Knee Arthroplasty: A Prospective, Double-blinded, Randomized Controlled Trial
Authors: Lai Yahao , Xu Hong , Su Qiang , Wan Xufeng , Yuan Mingcheng , Zhou Zongke 
Published: November 2021

Abstract
Background: Robot-assisted total knee arthroplasty (TKA) has been largely studied to confirm its advantages in terms of accurate component positioning, microembolus formation, less blood loss and so on, but is currently usually performed under tourniquet due to its longer operative time than conventional TKA. The aim of this study was to estimate the effects of tourniquet use in robot-assisted TKA on blood loss, pain, functional recovery, and complications.Methods: Patients scheduled for robot-assisted TKA were prospectively randomized into a tourniquet or non-tourniquet group (each n = 14). The primary outcome measure was blood loss. The secondary outcome measures were operation time; visual analog scale (VAS) pain scores; time to achieve the first straight-leg raise; swelling of the thigh, knee, and calf; range of motion; Hospital for Special Surgery score; length of stay; and postoperative complications. Results: There was no significant difference in total blood loss between the tourniquet and non-tourniquet groups (738.57 ± 276.158 vs. 866.85 ± 243.422 ml, P = 0.061). The tourniquet group showed significantly lower intraoperative blood loss (P < 0.001), but higher hidden blood loss (P = 0.002). The non-tourniquet group showed better knee range of motion on POD 1-3 (all P < 0.001), less thigh swelling on PODs 2 and 3 (P < 0.05), earlier straight-leg raising (P = 0.044), and shorter length of stay (P = 0.044). Thigh pain VAS score at 1 month after surgery was significantly greater in the tourniquet group (P < 0.001), as was knee pain during activity and at rest on PODs 2-3 (all P < 0.05). The tourniquet group also showed a significantly higher rate of tension blisters (28.8% vs. 7.1%, P = 0.038). Conclusions: Tourniquet use during robot-assisted TKA dose not reduce total blood loss and it appears to increase postoperative pain, aggravate muscle injury, and prolong postoperative recovery.Trial registration: ChiCTR, ChiCTR2100041800. Registered 5 January 2021, http://www.chictr.org.cn/index.aspx
An Improved Biogeography-based Optimization with Hybrid Migration and Feedback Differential Evolution and its Performance Analysis
Authors: Zhang Ziyu , Gao Yuelin , Li Jiahang , Zuo Wenlu 
Published: November 2021

Abstract
Biogeography-based optimization (BBO) is not suitable for solving high-dimensional or multi-modal problems. To improve the optimization efficiency of BBO, this study proposes a novel BBO variant, which is named ZGBBO. For the selection operator, an example learning method is designed to ensure inferior solution will not destroy the superior solution. For the migration opeartor, a convex migration is proposed to increase the convergence speed, and the probability of finding the optimal solution is increased by using opposition-based learning to generate opposite individuals. The mutation operator of BBO is deleted to eliminate the generation of poor solutions. A differential evolution with feedback mechanism is merged to improve the convergence accuracy of the algorithm for multi-modal and irregular problems. Meanwhile, the greedy selection is used to make the population always moves in the direction of a better area. Then, the global convergence of ZGBBO is proved with Markov model and sequence convergence model. Quantitative evaluations, compared with three self-variants, seven improved BBO variants and six state-of-the-art evolutionary algorithms, experimental results on 24 benchmark functions show that every improved strategy is indispensable, and the overall performance of ZGBBO is better. Besides, the complexity of ZGBBO is analyzed by comparing with BBO, and ZGBBO has less computation and lower complexity.
Generation of nonlinearity in the electrical response of yeast suspensions
Authors: Muraji Masafumi , Tamura Keita , Tanaka Kenji , Shirafuji Tatsuru 
Published: November 2021

Abstract
The mechanism through which nonlinearity is generated in the response waveform of the electric current obtained by applying alternating current voltage to yeast suspension has not yet been elucidated. In this paper, we showed that the response waveform depends on the applied voltage and frequency. The results showed that distortion (nonlinearity) in the waveform increases as the applied voltage increases and/or the frequency decreases. We suggest a model for the generation of nonlinearity based on the influx of potassium ions into the cell via potassium ion channels and transporters in the membrane due to the applied voltage. Furthermore, we validated this model by simulating an electrical circuit.
Hypomagnesemia and Poor Glycemic Control Among Palestinian Type 2 Diabetic Patients: A Cross-Sectional Study
Authors: Hamarshih Mohmmad , Hamshari Suha , Nazzal Zaher , Snobar Farha Abu , Mletat Rawa , Mazen Ola Abu , Maraqa Beesan 
Published: November 2021

Abstract
Background: Hypomagnesemia has been shown to have a significant impact on both glycemic control and diabetes complications in type 2 Diabetes Mellitus (T2DM) patients. This study aims to assess the prevalence of hypomagnesemia in T2DM patients and find the association between serum magnesium levels and outcomes relevant to glycemic control and diabetic complications.Methods: A cross-sectional study was conducted and included 373 patients (222 males and 151 females). Serum magnesium levels were measured by the Colorimetric Endpoint Method using the Cobas C501system. Hypomagnesemia was determined to be a serum magnesium level <1.6 mg/dL. In addition, the following data were also obtained; patients' characteristics, anthropometric measurements, smoking status, HbA1c, co-morbidities, and therapeutic management. Results: Patients' mean age was 56.2 ±10.8 years, 24.6% were smokers, and most were overweight or obese. About 60% have a history of hypertension, and the majority have had diabetes for more than ten years. Their mean HbA1c level was 8.5±2. The prevalence of hypomagnesemia was 11% (95% CI: 8%-14.6%). It was found to be significantly higher among females (adjusted OR: 2.7, 95%CI: 1.2%-5.8%), patients with HbA1c ≥ 8% (adjusted OR: 2.4, 95%CI: 1.1%-5.5%), and patients with a history of diabetic retinopathy (adjusted OR: 2.7, 95%CI: 1.1%-7.1%). Conclusions: the study showed that hypomagnesemia is more prevalent in females and is associated with diabetic retinopathy and poor glycemic control. Having a sufficient magnesium level may be associated with better glycemic control and a reduced occurrence of complications.
Deep Learning Model of Dock by Dock Process Significantly Accelerate the Process of Docking-Based Virtual Screening
Authors: Ma Wei , Xie Qin , Zhang Jianhang , Li Shiliang , Deng Xiaobing , Xu Youjun , Zhang Weilin 
Published: November 2021

Abstract
Docking-based virtual screening (VS process) selects ligands with potential pharmacological activities from millions of molecules using computational docking methods, which greatly could reduce the number of compounds for experimental screening, shorten the research period and save the research cost. Howerver, a majority of compouds with low docking scores could waste most of the computational resources. Herein, we report a novel and practical docking-based machine learning method called MLDDM (Machince Learning Docking-by-Docking Models). It is composed of a regression model and a classification model that simulates a classical docking by docking protocol ususally applied in many virtual screening projects. MLDDM could quickly eliminate compounds with low docking scores and the retained compounds with potential high docking scores would be examined for further real docking program. We demonstrated that MLDDM has a good ability to identify active compounds in the case studies for 10 specific protein targets. Compared to pure docking by docking based VS protocol, the VS process with MLDDM can achieve an over 120 times speed increment on average and the consistency rate with corresponding docking by docking VS protocol is above 0.8. Therefore, it would be promising to be used for examing ultra-large compound libraries in the current big data era.
A Modified Open-Door Laminoplasty with Reconstruction of the Cervical Posterior Ligamental Complex to Decrease Axial Pain in Cervical Spondylotic Myelopathy
Authors: Zhang Yayun , Chen Liangxi , Li Hao , Gao Xianlei , Zhao Hongwei , Pan Xin , Zhao Hua 
Published: November 2021

Abstract
Background:Cervical spondylotic myelopathy patients with multiple segments are usually treated with the posterior approach. But expansive open laminoplasty (ELAP) often results in heavy, rigid, and acid bilges feelings in the neck, shoulder, and back, collectively known as axial symptoms. Objective: To evaluate the effect of modified posterior cervical ligament complex reconstruction and single-door laminoplasty with titanium plate fixation on postoperative axial symptoms in patients. Methods: A retrospective analysis conducted from June 2016 to March 2018 collected more than 132 cases of cervical spondylotic myelopathy at our institute. Group A includes 74 patients and Group B includes 58 patients who use different surgery method. Gender, age, operation time, intraoperative blood loss, post-operative drainage volume, and follow-up time, Visual analogue scoring (VAS), cervical curvature index (CCI) and the cross-sectional area of the posterior cervical muscles of the two groups were recorded. Results: There was statistical significance in the incidence of axial pain 3 months after surgery (P =0.001), 6 months after surgery (P =0.006), and 1 year after surgery (P =0.015). And the VAS score was decreased in group A 1 week (P <0.0001), 1 ,3 month(P=0.0001), 6 months(P=0.0076), and 1 year(P=0.0085) post-surgery compared to group B. Also the CCI and the posterior cervical muscle area between groups A and B (P < 0.0001).Conclusion: Modified single open-door laminoplasty could relieve cervical axial pain in patients with cervical spondylotic myelopathy.
Predictive Factors for the Long-Term Clinical Course in Patients with Rheumatoid Arthritis Receiving Second-Line Anti-Rheumatic Drugs in Real-World Practice: An Analysis Using Disease Activity Trajectory-Based Clustering Approach
Authors: Koo Bon San , Eun Seongho , Shin Kichul , Hong Seokchan , Kim Yong-Gil , Lee Chang-Keun , Yoo Bin , Oh Ji Seon 
Published: November 2021

Abstract
Background: The purpose of this study was to stratify patients with rheumatoid arthritis (RA) according to the trend of disease activity by trajectory-based clustering and to identify the predictive factors for treatment response and the switching patterns of biologics according to trajectory groups. Methods: We analysed the data from a nationwide RA cohort from the Korean College of Rheumatology Biologics and Targeted Therapy (KOBIO) registry. Patients treated with second-line disease-modifying anti-rheumatic drugs (DMARDs) were included. Trajectory modeling for clustering was used to group the disease activity trend. The predictive factors and switching patterns of biologics for each trajectory were investigated.Results: The trends in the disease activity of 688 RA patients were clustered into 4 groups: rapid decrease and stable disease activity (group 1, N = 319), rapid decrease followed by an increase (group 2, N = 36), slow and continued decrease (group 3, N = 290), and no decrease in disease activity (group 4, N = 43). In the multivariable analysis for predictive factors, current smoking (OR, 7.845; 95% CI 2.158–28.220), low hemoglobin (OR 0.694; 95% CI, 0.532–0.901), and high initial disease activity score according to the 28-joint assessment (DAS28) (OR, 2.397; 95% CI, 1.638–3.586) were significantly associated with group 4 compared with group 1. Group 1 had a higher proportion of patients who had never had switching (86.5%) and who were initially treated with non-TNF inhibitors (44.2%) compared with groups 2 (52.8% and 25%), 3 (50.3% and 23.4%), and 4 (25.6% and 18.6%).Conclusions: The trajectory-based approach was useful for clustering the disease activity in longitudinal data in patients with RA. Among the four trajectories, the group with sustained high disease activity was associated with current smoking, low hemoglobin, high initial DAS28, and frequent switching of biologics.
Photophysical and Electronic Properties of Congo Red Dye Embedded in Polyvinyl Alcohol as an Efficient Laser Optical Limiter: Enhancement the Electrical Conductivity and Dielectric Properties
Authors: Mohammed M.I. , Al-Assiri Mohammed , Zahran H.Y. , AlAbdulaal T.H , Harraz Farid A. , Al-Assiri M. S. , Yahia I.S. 
Published: November 2021

Abstract
High-quality polymeric composite films containing Congo red dye and PVA were prepared using the casting technique. The proposed Cr-doped PVA composites were examined using XRD, UV–visible optical, dielectric, and optical limiting effect techniques. The XRD patterns of CR-doped PVA composite films display diffraction peaks, which belong to CR organic dye. There was a broadening and reduction of the initial peak intensity in polymer composite films at higher doping concentrations. The band edge of CR: PVA composites reduced with a massive shift from 4.63 eV to 1.86 e, were Congo red controls the energy bandgap of the PVA polymer. Dielectric permittivity diminishes with increasing the frequency and achieves constant values at higher frequencies through the relation between the electric dipole and the electric field differences. Increasing the percentage ratio of the CR doping enhances the electrical conductivity and dielectric permittivities due to the large size of the CR molecules. The strong optical limiting of the CR-doped PVA composites is determined using different laser sources of 632.8 and 532 nm wavelengths. The amazing obtained results imply that CR: PVA composite films are very significant for applying large-scale CUT-OFF laser filters for medical optical and electronic device systems.
ENSO Influence on Regional Atmospheric Circulation and Thermodynamics Over Northern South America
Authors: Ruiz-Vásquez Melissa , Arias Paola A. , Martínez J. Alejandro 
Published: November 2021

Abstract
The interannual variability of hydroclimatic conditions in Northern South America (NOSA), specially precipitation, is mainly influenced by the El Niño-Southern Oscillation (ENSO). We explored potential mechanisms that affect precipitation occurrence in NOSA during El Niño and La Niña events over the period 1980-2019, using data from the ERA5 reanalysis. We looked at the atmospheric moisture contribution from different sources using the Dynamic Recycling Model to track water vapour trajectories. Interestingly, conditions with reduced precipitation during El Niño events can take place along with increased precipitable water. To understand this, we analyzed thermodynamic conditions in the atmosphere that are necessary for precipitation to occur over the region, such as convective available potential energy, convective inhibition, lifting condensation level and low--level relative humidity. With this approach, we found more favorable atmospheric conditions for the occurrence of precipitation during La Niña events, even if the content of water vapor was equal or even less than during El Niño events. We also looked at the structure of the regional Hadley circulation in both types of events and found a weakening of the rising motion of the cell during El Niño, which further reduces convective processes over this region. This study provides an integral picture of how precipitation anomalies over NOSA during ENSO events are related both to thermodynamic conditions and sources of atmospheric moisture.
Responses of water environmental indicators to climate conditions in the middle and lower reaches of Lijiang River
Authors: Zhu Dantong , Cheng Xiangju , Li Wuhua , Niu Fujun , Wen Jianhui 
Published: November 2021

Abstract
With global climate change and increasingly extreme weather conditions, the water environment of the Lijiang River Basin is facing huge threats. Past studies have mostly focused on large-scale areas or have regional characteristics. Therefore, this study is based on the meteorological, hydrological, and water quality data of the Lijiang River from 2012 to 2018, using the analysis method Spearman's rank correlation coefficient, sensitivity , and contribution rate to quantitative analysis of the relationship between climate conditions and water environment indicators. The results show that the oxidation and alkalinity of the water in the Lijiang River Basin gradually increase, and the intensity becomes stronger as it goes downstream. DO increase and the concentrations of COD Mn , BOD 5 , and NH 4 -N all decreased, and water quality improved year by year. The input of external pollution has led to an upward trend in TP in Yangshuo. DO is positively correlated with wind speed and negatively correlated with other climate indicators. NH 4 -N and TP are mainly affected by precipitation, streamflow, humidity, and sunshine duration, only sunshine duration is negatively correlated. Pollutants from Guilin to Yangshuo on both sides of the Lijiang River were carried by the surface runoff into the water body contain a certain amount of organic matter and acidic matter. Water environment indicators are not very sensitive to precipitation and streamflow, humidity and wind speed have higher sensitivity. Water temperature and sunshine duration have a positive effect on reducing NH 4 -N and TP. Various climate conditions can help reduce organic matter in the water body where there are few external sources and the opposite contribution with external sources. No climate condition can dominate one water environment indicator of two stations at the same time. The difference between Yangshuo and Guilin is mostly due to the input of external sources on both sides of the Lijiang River, which leads to the difference in sensitive climate conditions. Construction of non-point source pollution reduction facilities and sewage treatment measures are very necessary.
Consumption of ultra-processed foods is associated with abdominal obesity in rotating shift workers
Authors: Júnior Luiz Antônio Alves de Menezes , Fajardo Virgínia Capistrano , de Oliveira Jonathas Assis , Machado-Coelho George Luiz Lins , Pimenta Fausto Aloísio Pedrosa , de Oliveira Fernando Luiz Pereira , Neto Raimundo Marques do Nascimento , de Freitas Sílvia Nascimento , Meireles Adriana Lúcia 
Published: November 2021

Abstract

Background and aims: Higher intake of ultra-processed foods might be associated with an increased risk of obesity. Our objective was to evaluate the consumption of ultra-processed foods and their association with nutrient intake and excess body adiposity in shift workers.Design: Cross-sectional study conducted in 2016 with 238 male rotating shift workers. Dietary data is obtained by the 24-hour recall and classified according to processing by the NOVA classification system. Body adiposity indicators assessed were waist circumference and body mass index. Logistic regression models were built and adjusted for sociodemographic, lifestyle, and dietary variables.Results: Ultra-processed foods represented on average 22.3% of the total caloric value of the individuals' food consumption, with a maximum value of 66.9%. Participants with the highest tercile of ultra-processed foods consumed more carbohydrate (57%), protein (35%), total fat (96%), saturated fat (79%), cholesterol (68%), and sodium (44%) intake compared to the first tercile (p < 0.001). The most frequencies of ultra-processed foods consumed were bread (81.0%), followed by cookies (45.9%), sweetened beverages (45.7%), processed meats (46.8%), and margarine (46.8%). In multivariable analysis, the highest consumption of ultra-processed foods had 183% higher odds of abdominal obesity (OR = 2.83, 95%CI 1.29-6.22), compared with the lowest consumption. Conclusion: Ultra-processed foods are important contributors to the energy intake of these workers, and higher consumption of ultra-processed foods was associated with abdominal obesity.