bluuebunny/crossref_metadata_2025_split huggingface dataset:
Efficacy of radiotherapy to local recurrence and distant metastasis nasopharyngeal carcinoma: long-term retrospective multicenter study
Authors: li lu , Deng Mingyou , Ren Jianlan , Liao Wenjun , Zheng Liangjian , Ma Hui , Lang Jinyi , Feng Mei , Luo Yangkun 
Published: December 2024

Abstract

Purpose
The patients with locoregional recurrence and distant failure after initial treatment was a unique group of nasopharyngeal carcinoma(NPC). This patients population presented unique challenges in management: when and in what sequence to add radiotherapy(RT) to address locoregional and distant disease.
Methods
This retrospective multicenter investigation enrolled local recurrence and distant metastasis (rmNPC) from five hospitals. Kaplan-Meier and log-rank tests were utilized for survival assessment of patients with distinct profiles of recurrence and metastasis, as well as diverse treatment modalities. Additionally, we employed the Cox regression model to investigate the stand-alone risk factors influencing the survival of patients with local recurrence and distant metastasis (rmNPC).
Results
52 patients were enrolled in the study, the median follow up time was 68.3 months (range: 7-240 months). The median overall survival (OS) time was 23.4 months (range: 11.1–35.6 months), with 1, 2, 3, 4 and 5 years OS rates of 61.3%, 46.5%, 31.0%, 27.9%, 10.5% respectively. OS had no statistical difference in treatment modalities(p = 0.071), the median OS was 10.8 months (95% CI, 7.7 to 13.9) for chemotherapy alone, 24.2 months (95% CI, 8.9 to 39.4) for chemotherapy combined with PD-1 inhibitors, and 47.1 months (95% CI, 10.2 to 84.0) for chemotherapy combined with radiotherapy. Among patients with oligometastasis, those who received radiotherapy exhibited a prolonged OS (50.1 vs. 24.1 months, p = 0.021), whereas patients with polymetastasis who received radiotherapy failed to experience a substantial extension in OS (8.6 vs. 14.8 months, p = 0.168). Similarly, among patients with one-organ metastasis, radiotherapy was intricately linked to extended OS(50.1 vs. 24.1 months, p = 0.026), while patients with multiple organ metastases did not demonstrate a substantial OS benefit from radiotherapy (8.6 vs. 11.0 months, p = 0.831).
Conclusions
The combines radiotherapy shows the promising efficacy for rmNPC patients with one-organ metastasis or oligometastasis.
Developmental exposure to perfluorooctanoic sulfonate（PFOS） impairs the endometrial receptivity
Authors: Ren Rui , Zhou Xinyue , Jia Tianyu , Wang Bin , Liu Ahui , Song Ji , Wang Liyan , Jing Yuanxue , Yu Liulin , Shen Haofei , Zhang Xuehong , Gao Min 
Published: December 2024

Abstract

Perfluorooctanoic sulfonate (PFOS) is difficult to degrade and tends to accumulate in the body, which causes widespread concern. The expression of genes related to endometrial receptivity and the differentiation of human endometrial stromal cells (hESCs) were assessed in this study concerning PFOS. In this study, we investigated the effect of PFOS exposure on endometrial tolerance by cell and animal experiments. The activity against endometrial mesenchymal cells was significantly reduced by PFOS intervention, and the apoptosis flow assay results showed that PFOS significantly promoted cell death in a concentration-dependent manner. Transmission electron microscopy results revealed mitochondrial damage in the PFOS-intervened group, and WB results showed that the expression levels of endometrial tolerance-related proteins Homeobox A10 (HOXA10) and integrin beta3 (ITGB3) were decreased, and the expression level of Forkhead box O1 (FOXO1) protein was increased. Animal studies have shown that PFOS can affect the locomotor cycle in mice, and significant damage to pinopodes morphology was observed after PFOS exposure administration. In the present study, we found that PFOS may synergistically affect the viability of endometrial mesenchymal stromal cells through accumulation in vivo, and that PFOS may contribute to the failure of embryo implantation by affecting mitochondrial function and consequently endometrial permissive sites.
High Abundance of Gut Bacteroides in Early Childhood correlates with Microbiome Stability and Resilience after Antibiotic Treatment and with Increased Risk for Childhood Infections
Authors: Hauptmann Matthias , Gottschick Cornelia , Muthukumarasamy Uthayakumar , Klee Bianca , Strowig Till , Mikolajczyk Rafael , Schaible Ulrich Emil 
Published: December 2024

Abstract

Background
Early childhood events, up to the age of two, are critical for the development of the microbiome and balanced immunity later in life. We investigated whether susceptibility to infections and microbiome resilience after antibiotic treatment are associated with key taxa in the early childhood microbiota. Therefore, we performed longitudinal microbiota analysis from stool samples of children within the German LöwenKIDS intensified subcohort. According to the exposure to antibiotic treatment, sample groups were defined as never-treated controls, 45–225 days pre-treatment (pre45-225), 0–30 days pre-treatment (pre0-30), 0–30 days post-treatment (post0-30), or > 90 days post-treatment and age > 540 days (post > 90).
Results
1176 stool samples of 162 children were included in our analysis, of which 49 children received antibiotics. Using generalized linear mixed models adjusted for age, we show that high abundance of Bacteroides was associated with receiving antibiotic treatment later, while low Bacteroides abundance before treatment was associated with low alpha diversity and increased beta diversity post treatment.
Conclusions
Our data suggest a key role of the genus Bacteroides for the susceptibility to infections requiring antibiotic treatment and for microbiome stability and resilience in early childhood.
Treatment of limb skin defect with self-made skin distractor
Authors: Lei Gao , Kai Li , Yanlong Zhang , Hongrun Wang , Shibo Liu , Yong Wang , Aqin Peng 
Published: December 2024

Abstract

Objective To investigate the effect of self-made skin distractor in the treatment of limb skin defects.
Methods From September 2018 to January 2020, 15 cases of limb skin defects (16 wounds) were treated with self-designed skin distractor, including 13 males and 2 females, with an average age of 42 years (range, 9-73 years). The smallest area of skin defect was 6cm×3cm, the largest was 32cm ×7cm, with an average of 72.4cm2. The wound healing was observed, complications and the time of use of the distractor were recorded.
Results All patients were followed up for 6-12 months with an average of 9.2 months. Of the 16 wounds in 15 patients, 2 wounds were closed in one stage during operation, 13 wounds were delayed in primary closure. Skin incision occurred in 1 case, but the wound closure was not affected；One wounds were not closed completely, because the skin was cut by Kirschner wire, and could not be stretched continously. The remaining wounds were closed by skin grafting. Another case also had skin cutting was occurred in another case, but the wound closure was not affected. No skin stretch was terminated due to pain. All wounds were healed. The elasticity and color of the skin on the wound surface were not different from the surrounding skin. The movement of the adjacent joints was basically normal.
Conclusion The self-made skin stretcher is simple in structure and easy to use. It provides a reliable and effective method for the treatment of skin defects of limbs.
The Establishing of Stellate Ganglion Regulation Model in Mice by Using Infrared Polarized Light Irradiation
Authors: Zhao Kaixuan , Zhang Haoyue , Liu Yanbo , Zhou Ying , Zhi Juan , Wang Qianyu , Yang Dong 
Published: December 2024

Abstract

Objective
To explore the feasibility of establishing a mouse stellate ganglion (SG) regulation model through infrared polarized light (IPL) irradiation of the SG, and preliminarily evaluate its effects on SG function and related physiological indicators.
Methods
BALB/c mice were randomly divided into control, Sham surgery, and IPL groups, with 8 mice in each group. A ZZIR-ID therapeutic device was used to directly irradiate bilateral SG regions of IPL group mice, with wavelength 980 nm, power density 1000 mW/cm2, 15 minutes each time, every other day for 6 times. The control group received no treatment, while the Sham surgery group received IPL irradiation on the top of the head. Horner's syndrome manifestations were observed and eye temperature was measured before and immediately after treatment. Heart rate changes were continuously recorded.
Results
Compared with the control and Sham surgery groups, the incidence of Horner's syndrome in the IPL group increased significantly (P < 0.05), manifesting as bilateral ptosis and enophthalmos, lasting about 2 hours. Immediately after treatment, eye temperature in the IPL group increased significantly compared to pre-treatment (P < 0.05). Heart rate in the IPL group decreased significantly 30 minutes post-treatment compared to pre-treatment (P < 0.01), lasting 1–2 hours. There was no statistically significant difference in weight changes between groups (P > 0.05).
Conclusion
IPL irradiation of SG can effectively induce Horner's syndrome in mice, elevate eye temperature, reduce heart rate, and exert certain anti-inflammatory immunomodulatory effects. This provides experimental evidence for IPL as a novel method to establish SG regulation models.
Heat Transfer Visualization of Transitional Growth of Turbulent Spot on a Wedge in Mach 5.2 Hypersonic Flow Using Fast-Response TSP
Authors: Yoshikawa Kosuke , Ozawa Hiroshi 
Published: December 2024

Abstract

In the present study, the propagation velocity and lateral spreading angle of turbulent spots on a wedge in transient hypersonic boundary layer flows were investigated and characterized by measuring the heat flux distribution using a fast-response Temperature-Sensitive Paint (TSP) in a shock tunnel facility. The shock tunnel was operated under the over-tailored condition and provided with the low- to high unit Reynolds number during a test duration, which was realized by the contact surface arrival in the shock tube that changed the temperature and density of the reservoir gas. Power Spectral Density (PSD) estimated from the pressure recordings may indicate that the boundary layer flow was transitional. In TSP measurements, the global heat flux distribution on the wedge was accurately obtained qualitatively. Surprisingly, turbulent spots were visualized using TSP for each unit Reynolds number condition. The propagation velocities at the head, peak heat flux point, and tail of turbulent spots for low- and high unit Reynolds number conditions were approximated from the TSP images to be 87 ~ 96%, 69 ~ 73%, and 52 ~ 57% of the boundary layer edge velocities at each condition, respectively. These results were in good agreement with the available data in previous investigations. The lateral spreading angle of turbulent spots was also measured to be 5˚~10˚ from the TSP images. This study showed that the fast-response TSP had the ability to visualize the temporally resolved turbulent spots by measuring the heat flux on the wedge.
Ca2+-dependent H2O2 response in roots and leaves of barley - A transcriptomic investigation
Authors: Bhattacharyya Sabarna , Bleker Carissa , Meier Bastian , Giridhar Maya , Rodriguez Elena Ulland , Braun Adrian Maximilian , Peiter Edgar , Vothknecht Ute C. , Chigri Fatima 
Published: December 2024

Abstract

Background
Ca2+ and H2O2 are second messengers that regulate a wide range of cellular events in response to different environmental and developmental cues. In plants, stress-induced H2O2 has been shown to initiate characteristic Ca2+ signatures; however, a clear picture of the molecular connection between H2O2-induced Ca2+ signals and H2O2-induced cellular responses is missing, particularly in cereal crops such as barley. Here, we employed RNA-seq analyses to identify transcriptome changes in roots and leaves of barley after H2O2 treatment under conditions that inhibited the formation of cytosolic Ca2+ transients. To that end, plasma membrane Ca2+ channels were blocked by LaCl3 application prior to stimulation of barley tissues with H2O2.
Results
We examined the expression patterns of 4246 genes that had previously been shown to be differentially expressed upon H2O2 application. Here, we further compared their expression between H2O2 and LaCl3+H2O2 treatment. Genes showing expression patterns different to the previous study were considered to be Ca2+-dependent H2O2-responsive genes. These genes, numbering 331 in leaves and 1320 in roots, could be classified in five and four clusters, respectively. Expression patterns of several genes from each cluster were confirmed by RT-qPCR. We furthermore performed a network analysis to identify potential regulatory paths from known Ca2+-related genes to the newly identified Ca2+-dependent H2O2 responsive genes, using the recently described Stress Knowledge Map. This analysis indicated several transcription factors as key points of the responses mediated by the cross-talk between H2O2 and Ca2+.
Conclusion
Our study indicates that about 70% of the H2O2-responsive genes in barley roots require a transient increase in cytosolic Ca2+ concentrations for alteration in their transcript abundance, whereas in leaves, the Ca2+ dependency was much lower at about 33%. Targeted gene analysis and pathway modeling identified not only known components of the Ca2+ signaling cascade in plants but also genes that are not yet connected to stimuli-associated signaling. Potential key transcription factors identified in this study can be further analyzed in barley and other crops to ultimately disentangle the underlying mechanisms of H2O2-associated signal transduction mechanisms. This could aid breeding for improved stress resistance to optimize performance and productivity under increasing climate challenges.
Long-Term Outcomes of Corpus Callosotomy in Adult Patients with Drug-Resistant Epilepsy; Results from a Single Neurosurgical Center in Mexico
Authors: Pichardo-Rojas Diego , Pech-Cervantes Claudio Hiram , Macias-Lopez Jonathan Ulises , Camarena-Rubio Karen Janely , Río-Quiñones Manuel Alejandro Del , Ballesteros-Herrera Daniel , Paredes-Aragon Elma , Hernandez-Vanegas Laura E , Pichardo-Rojas Pavel S , Mejía-Pérez Sonia Iliana 
Published: December 2024

Abstract

Objective Corpus callosotomy (CC) is a commonly indicated palliative surgery for Drug-Resistant Epilepsy (DRE). While younger age at the time of surgery is a well-established positive prognostic factor, there is limited evidence on long-term outcomes in adult patients with DRE. A study that evaluates seizure control at various follow-up points is necessary to better understand the outcomes of CC.Methods In this retrospective longitudinal cohort we included adult CC patients operated by our institution’s epilepsy surgery service between 1993 and 2023. We compiled epilepsy etiology, preoperative seizure frequency, and postoperative seizure outcomes at < 1-year follow-up, 1–3 years follow-up, and > 3 years of follow-up. Our outcomes were seizure freedom and response rate (50% frequency decrease from preoperative).Results We analyzed data from 63 patients. The most common indication for CC was DRE due to Lennox-Gastaut Syndrome. Median seizure frequency had a significant decrease from preoperative (70 seizures per month [spm], IQR = 16–210) to the 1-year follow-up (4 spm, IQR = 0–20, p = < 0.0001), 1–3 years follow-up (8 spm, IQR = 1–30, p = < 0.0001), and > 3 years of follow-up (7 spm, IQR = 2.25-30, p = < 0.0001). Seizure freedom at one year postoperative decreased from 25.4–9.7% at 1–3 years (p = 0.032), while the overall response rate of our sample remained consistent, being 81% at 1-year follow-up and 70% at the long-term follow-up (p = 0.20).Conclusion We identified that CC effectively decreases seizure frequency in adult patients with DRE. The reduction of seizure frequency after CC remained consistent after the first postoperative year. Prospective clinical trials are needed to better understand the long-term outcomes of CC in adults.
Spatial accuracy of dose delivery significantly impacts the planning target volume margin in linear accelerator-based intracranial stereotactic radiosurgery
Authors: Takahashi Yuta , Oshika Riki , Tachibana Rie , Shirai Katsuyuki , Asakura Hiroshi , Miyazaki Masayoshi , Sagawa Tomohiro , Takahashi Shinichi , Kuwae Tsunekazu , Kojima Hironori , Nishiyama Shiro , Nemoto Hikaru , Ishihara Yoshitomo , Umeda Mariko , Kijima Kotaro , Kobayashi Daisuke , Suzuki Keiji , Nozawa Yuki , Hoshida Kento , Kitagawa Tomoki , Endo Hiromitsu , Matsunaga Yuki , Itagaki Hiroya , Ishida Mayumi , Kanahara Shigeru , Horita Ryo , Hori Daisuke , Tachibana Hidenobu 
Published: December 2024

Abstract

The impact of three-dimensional (3D) dose delivery accuracy of C-arm linacs on the planning target volume (PTV) margin was evaluated for non-coplanar intracranial stereotactic radiosurgery (SRS). A multi-institutional 3D starshot test using beams from seven directions was conducted at 22 clinics using Varian and Elekta linacs with X-ray CT-based polymer gel dosimeters. Variability in dose delivery accuracy was observed, with the distance between the imaging isocenter and each beam exceeding 1 mm at one institution for Varian and nine institutions for Elekta. The calculated PTV margins for Varian and Elekta linacs that could cover the gross tumor volume with 95% probability at 95% of the institutions were 2.3 and 3.5 mm, respectively, in the superior–inferior direction. However, with multifactorial system management (i.e., high-accuracy 3D dose delivery with rigorous linac quality assurance, strict patient immobilization, and high intra-fractional positioning accuracy), these margins could be reduced to 1.0 mm and 1.5 mm, respectively. The findings indicate significant millimeter-level variability in 3D dose delivery accuracy among linacs installed in clinical settings, but effective system management can achieve PTV margins below 2 mm, suitable for SRS applications.
Temperature Monitoring Under Regional Anesthesia: Tympanic Temperature vs Temple Touch Pro™ Core
Authors: Racca Fabrizio , Ruvolo Domenico , Pogliano Marco , Caputo Giorgia , Savarese Benedetta , Racca Giulia , Conti Elena , Cascio Nunzio Dario , Barberis Luigi , Mahajan Aman , Planinsic Raymond , Longhitano Yaroslava , Via Luigi La , Deana Cristian , Zanza Christian , Leo Mirco 
Published: December 2024

Abstract

BACKGROUND
Perioperative hypothermia is a common finding with potential associated complications. Tympanic temperature measurement (Temptympanic) is the most commonly used method for assessing core temperature in patients undergoing neuraxial anesthesia. The Temple Touch Pro™ (TTP™) is a new, totally non-invasive core temperature monitoring device, allowing temperature measurement in conscious patients undergoing regional anesthesia. We tested the hypothesis that this tool estimates core temperature to an accuracy within 0.5ºC.
METHODS
Core temperature was continuously monitored in adult surgical patients by positioning the TTP™ sensor over the skin above the temporal artery (TempTTP). Reference temperature was Temptympanic. Accuracy of the two methods was assessed by Bland-Altman comparisons of differences with multiple measurements and by Pearson correlation coefficient analysis.
RESULTS
A total of 199 pairs of TempTTP and Temptympanic were measured for 23 patients. The 92.5% difference in temperature measured between TTP™ and the reference Temptympanic is within the interval of 0.5°C. Bland-Altmann analysis showed a TempTTP mean bias of 0.20°C with 95% limits of agreement within − 0.44°C to + 0.85°C compared to Temptympanic, indicating good agreement between the two methods. Good agreement was also supported by a Pearson correlation coefficient of 0.74. Furthermore, the TTP™ system demonstrated superior performance compared to the reference method, as it maintained a more stable core temperature.
CONCLUSIONS
We consider the non-invasive TTP™ system as a reasonable tool for perioperative temperature monitoring for conscious patients undergoing regional anesthesia.
MRI quantiﬁed perivascular space metrics as imaging biomarkers for assessing the severity of cognitive impairment and sleep disturbance in young adults with long-time mobile phone use through machine learning approaches
Authors: Li Li , Wu Jiaojiao , Li Bin , Hua Rui , Shi Feng , Chen Lizhou , Wu Yeke 
Published: December 2024

Abstract

Emerging evidence has linked long-time mobile phone use (LTMPU) with cognitive impairment and sleep issues, with MRI-detected enlarged perivascular spaces (EPVSs) serving as markers for these conditions. Our study seeks to develop predictive model using MRI-based PVS measurements and machine learning to assess cognitive impairment, subjective sleep quality, and excessive daytime sleepiness in young adults with LTMPU. Eighty-two participants were included, deep learning algorithms were used to segment EPVS lesions and extract quantitative metrics. Training and testing datasets were randomly assigned to perform radiomics analysis, where EPVS metrics combined with sex and age were used to select the most valuable features for model construction. Finally, a Gaussian process model was constructed based on six features for assessing cognitive impairment, yielding an AUC of 0.818 (95% confidence interval [CI] 0.610-1) in the testing dataset. For sleep quality and sleepiness, two decision tree (DT) models using six features achieved an AUC value of 0.826 (95% CI 0.616-1) and 0.875 (95% CI 0.718-1) in the testing dataset respectively. Our study leveraged MRI-based PVS metrics and machine learning to assess the severity of cognitive impairment and sleep problems in young adults with LTMPU, and sheds light on a potential link between PVS and sleepiness.