bluuebunny/crossref_metadata_2025_split huggingface dataset:
MiRNA‐339‐5p promotes isoproterenol‐induced cardiomyocyte hypertrophy by targeting VCP to activate the mTOR signaling
Authors: Bi Xueying , Zhang Yuhong , Yu Youhui , Yuan Jing , Xu Siting , Liu Fang , Ye Jiantao , Liu Peiqing 
Published: December 2021

AbstractMicroRNAs (miRNAs) regulate multiple biological processes and participate in various cardiovascular diseases. This study aims to investigate the role of miR‐339‐5p in cardiomyocyte hypertrophy and the involved mechanism. Neonatal rat cardiomyocytes (NRCMs) were cultured and stimulated with isoproterenol (ISO). The hypertrophic responses were monitored by measuring the cell surface area and expression of hypertrophic markers including β‐myosin heavy chain (β‐MHC) and atrial natriuretic factor (ANF). Bioinformatic prediction tools and dual‐luciferase reporter assay were performed to identify the target gene of miR‐339‐5p. Quantitative real‐time polymerase chain reaction and western blot analysis were used to determine the levels of miR‐339‐5p and its downstream effectors. Our data showed that miR‐339‐5p was upregulated during cardiomyocyte hypertrophy triggered by ISO. MiR‐339‐5p overexpression resulted in enlargement of cell size and increased the levels of hypertrophic markers. In contrast, inhibition of miR‐339‐5p significantly attenuated ISO‐induced hypertrophic responses of NRCMs. Valosin‐containing protein (VCP), a suppressor of cardiac hypertrophy via inhibiting mechanistic target of rapamycin (mTOR) signaling, was validated as a target of miR‐339‐5p. MiR‐339‐5p suppressed VCP protein expression, leading to elevated phosphorylation of mTOR and ribosomal protein S6 kinase (S6K). VCP depletion activated the mTOR/S6K cascade and could compromise the anti‐hypertrophic effects of miR‐339‐5p inhibitor. Additionally, the hypertrophic responses caused by miR‐339‐5p was alleviated in the presence of mTOR inhibitor rapamycin. In conclusion, our research revealed that miR‐339‐5p promoted ISO‐induced cardiomyocyte hypertrophy by targeting VCP to activate the mTOR signaling, suggesting a promising therapeutic intervention by interfering miR‐339‐5p.
Realistic Operando‐DRIFTS Studies on Cu/ZnO Catalysts for CO2 Hydrogenation to Methanol – Direct Observation of Mono‐ionized Defect Sites and Implications for Reaction Intermediates
Authors: Fehr Samuel M. , Nguyen Karin , Krossing Ingo 
Published: December 2021

AbstractOur recent study in this journal highlighted misassignments of surface intermediates of diffuse reflectance infrared Fourier transform spectroscopy (DRIFTS) measurements of published Cu/ZnO (CZ) catalyst systems for methanol synthesis. Here we investigate a recent and very active CZ system, in part promoted by oxidative fluorination (i. e., a Cu/ZnO1–x/ZnF2 system), with realistic operando‐DRIFTS measurements of a 3H2/CO2 gas feed (30 bar, 513 K). This DRIFTS setup is linked to an online GC analysis system and the catalytic performance of the Cu/ZnO1–x catalyst showed a similar performance of the catalysis process in the DRIFTS cell as in the catalyst test station. In the DRIFTS measurements, a very broad absorption band with a maximum at about
1500 cm−1 (=I1500) is evident. This I1500 band is absent in nitrogen; its intensity increases in pure hydrogen and is particularly high during methanol synthesis. I1500 results from photoionization of an electron residing in a mono‐ionized oxygen vacancy VO+ in the ZnO1–x part of the Cu/ZnO1–x catalyst. Consequently, the I1500 band intensity provides information on the extent of the strong metal‐support interaction SMSI. Therefore, measurement of the I1500 band intensity could be a novel and efficient tool to characterize any CZ‐based catalyst systems online. Mechanistically, the maximum VO+ photoionization I1500 band intensity in a 3H2/CO2 gas stream is coupled to the reaction of CO2 giving the CO2−. radical anion intermediate that is rapidly trapped in the VO2+ site formed. This trapped intermediate may react by hydrogen migration to the well‐known surface formate (1603, 1371, and 1314 cm−1). However, the long‐lived formate with this spectroscopic signature is only a spectator. It is also visible by DRIFTS in catalyst samples that in the same setup do not produce methanol (GC). By contrast, the band intensities of the surface species at 1759, 1691, 1457 and 1398 cm−1 are directly connected to methanol production and the applied WHSV. Therefore, only these surface species are relevant for CO2 hydrogenation to methanol at higher pressure and likely represent true reaction adsorbates/intermediates.
Der Human Factor bei der Anlagensicherheit
Authors: Gandert Etwina 
Published: December 2021

AbstractDie Sicherheit einer chemischen Anlage hängt einerseits von technischen Einrichtungen und andererseits von Entscheidungen der handelnden Personen ab. Beides, Mensch und Prozess sind daher beim Aufbau der Sicherheitsbausteine zu betrachten. Das neue Dechema‐Seminar behandelt Lücken, die das risikobasierte Process Safety Management hinterlässt und durch die es überproportional viele Ereignisse aus vermeintlich ‘einfachen’ Gründen gibt. Aus der Veranstaltung im September konnten die Teilnehmenden pragmatische Hilfen und Werkzeuge mitnehmen, die in der Praxis zum Verhindern von Ereignissen beitragen und die sich damit positiv auf Sicherheit, Effizienz und Effektivität auswirken.
Schutzkonzepte für ‐ Batteriespeichersysteme
Authors: Saling Carlo , Kemmling Alexander 
Published: December 2021

AbstractLithiumionen‐Akkumulatoren – auch als Lithium‐ionen‐Batterien bezeichnet – sind aus unserem täglichen Leben nicht mehr wegzudenken. Sie bewähren sich seit vielen Jahren aufgrund ihres hohen Wirkungsgrads als wiederaufladbare Speicher in Kleingeräten wie z. B. Handys, Notebooks, Kameras, Werkzeugen und dem Modellbau. Inzwischen haben Lithium‐ionen‐Batterien ihren Siegeszug auch auf Elektroautos, Fahrräder, Flur‐förderfahrzeuge und Batteriespeichersysteme (BESS = Battery Energy Storage Systems) ausgedehnt.
Intra‐abdominal hemorrhage as a rare complication of endoscopic ultrasonography: A case report
Authors: Ishigaki Kazunaga , Nakai Yousuke , Inokuma Akiyuki , Suzuki Yukari , Noguchi Kensaku , Kanai Sachiko , Suzuki Tatsunori , Sato Tatsuya , Hakuta Ryunosuke , Saito Kei , Saito Tomotaka , Takahara Naminatsu , Hamada Tsuyoshi , Mizuno Suguru , Kogure Hirofumi , Nakai Yudai , Fujishiro Mitsuhiro 
Published: December 2021

AbstractIntra‐abdominal hemorrhage after endoscopic ultrasonography (EUS) is an uncommon complication, which can lead to potentially fatal outcomes. We describe a case of intra‐abdominal hemorrhage due to left gastric arterial bleeding after EUS. The patient developed severe epigastric pain 10 h after diagnostic EUS for pancreatic cysts. Contrast‐enhanced computed tomography revealed extravasation from the left gastric artery as well as a hematoma in the lesser omentum, which was confirmed by emergent angiography. Spontaneous hemostasis was obtained without embolization and the patient did not have further episodes of intra‐abdominal hemorrhage. Endoscopists should be aware of this rare but serious complication after endoscopic procedures.
Comparative riverscape genomics of the rainbow darter (Etheostoma caeruleum) in glaciated and unglaciated environments
Authors: Luiken Jon M. , Gamble Tony , Berendzen Peter B. 
Published: December 2021

AbstractPeriodic glaciation during the Quaternary period shaped the contemporary riverscape and distribution of freshwater fishes in the Mississippi River drainage of central North America. The rainbow darter (Etheostoma caeruleum) is a member of this ichthyofauna and has a disjunct distribution in glaciated and unglaciated environments west of the Mississippi River. Based on glacial history of the region, there are different expectations on the observed spatial genetic structure of populations in these environments. The aim of this study was to utilize genome‐wide SNP data to compare the population genomic structure of the rainbow darter in river networks with disparate glacial histories; the Volga River in the glaciated upper Mississippi River basin and the Meramec River in the unglaciated Ozark Plateau. Individuals were sampled from localities within each river system at distances dictated by the organismal life history and habitat preferences. Riverscape analyses were performed on three datasets: total combined localities of both rivers and one for each river independently. The results revealed a lasting influence of historic glaciation on the population genomic structure of rainbow darter populations. There was evidence of population expansion into the glaciated northern region following glacial retreat. The population genetic signature within the Volga River did not fit expectations of the stream hierarchy model, but revealed a pattern of repeated colonization and extirpation due to cyclic glaciation. The population within the unglaciated Meramec River adhered to the stream hierarchy model, with a directional order of genetic diversity based on the life history and habitat preferences of the species. These results demonstrate the importance of considering the geologic and climatic history of a region as well as the life history of an organism when interpreting spatial genetic patterns.
Energy‐sparing by 2‐methyl‐2‐thiazoline protects heart from ischaemia/reperfusion injury
Authors: Nishi Masahiro , Ogata Takehiro , Kobayakawa Ko , Kobayakawa Reiko , Matsuo Tomohiko , Cannistraci Carlo Vittorio , Tomita Shinya , Taminishi Shunta , Suga Takaomi , Kitani Tomoya , Higuchi Yusuke , Sakamoto Akira , Tsuji Yumika , Soga Tomoyoshi , Matoba Satoaki 
Published: December 2021

AbstractAimsCardiac ischaemia/reperfusion (I/R) injury remains a critical issue in the therapeutic management of ischaemic heart failure. Although mild hypothermia has a protective effect on cardiac I/R injury, more rapid and safe methods that can obtain similar results to hypothermia therapy are required. 2‐Methyl‐2‐thiazoline (2MT), an innate fear inducer, causes mild hypothermia resulting in resistance to critical hypoxia in cutaneous or cerebral I/R injury. The aim of this study is to demonstrate the protective effect of systemically administered 2MT on cardiac I/R injury and to elucidate the mechanism underlying this effect.Methods and resultsA single subcutaneous injection of 2MT (50 mg/kg) was given prior to reperfusion of the I/R injured 10 week‐old male mouse heart and its efficacy was evaluated 24 h after the ligation of the left anterior descending coronary artery. 2MT preserved left ventricular systolic function following I/R injury (ejection fraction, %: control 37.9 ± 6.7, 2MT 54.1 ± 6.4, P < 0.01). 2MT also decreased infarct size (infarct size/ischaemic area at risk, %: control 48.3 ± 12.1, 2MT 25.6 ± 4.2, P < 0.05) and serum cardiac troponin levels (ng/mL: control 8.9 ± 1.1, 2MT 1.9 ± 0.1, P < 0.01) after I/R. Moreover, 2MT reduced the oxidative stress‐exposed area within the heart (%: control 25.3 ± 4.7, 2MT 10.8 ± 1.4, P < 0.01). These results were supported by microarray analysis of the mouse hearts. 2MT induced a transient, mild decrease in core body temperature (°C: −2.4 ± 1.4), which gradually recovered over several hours. Metabolome analysis of the mouse hearts suggested that 2MT minimized energy metabolism towards suppressing oxidative stress. Furthermore, 18F‐fluorodeoxyglucose‐positron emission tomography/computed tomography imaging revealed that 2MT reduced the activity of brown adipose tissue (standardized uptake value: control 24.3 ± 6.4, 2MT 18.4 ± 5.8, P < 0.05). 2MT also inhibited mitochondrial respiration and glycolysis in rat cardiomyoblasts.ConclusionsWe identified the cardioprotective effect of systemically administered 2MT on cardiac I/R injury by sparing energy metabolism with reversible hypothermia. Our results highlight the potential of drug‐induced hypothermia therapy as an adjunct to coronary intervention in severe ischaemic heart disease.
Mechanisms of Reaction Between Co(II) Complexes and Peroxymonosulfate
Authors: Shamir Dror , Meyerstein Dan , Katsaran Dmitry , Pochtarenko Lyudmila , Yardeni Guy , Burg Ariela , Albo Yael , Kornweitz Haya , Zilbermann Israel 
Published: December 2021

AbstractAdvanced oxidation technologies often use peroxymonosulfate in the presence of CoIIaq. It is commonly assumed that the reaction of Co(H2O)62+ with HSO5− yields CoIIIaq and SO4.−. DFT results point out that first CoII(SO5)(H2O)2 is formed. The homolysis of CoII(SO5)(H2O)2 to yield (H2O)CoII(SO5)OH.+SO4.−, is exothermic but has a large activation energy. However the cobalt is not oxidized in this reaction. CoII(SO5)(H2O)2 reacts with a second HSO5− to form CoII(SO5)2(H2O)2− that decomposes via disproportionation of the monoperoxysulfate ions without oxidation of the central cobalt ion. Surprisingly even in the presence of ligands, L, that stabilize CoIII, i. e., pyrophosphate; tri‐polyphosphate and ATP, the experimentally observed reaction mechanism involves the formation of LCoII‐OOSO3aq which then reacts with another HSO5− to form LCoII‐(OOSO32−)2. The latter complex decomposes via disproportionation of the monoperoxysulfate ligands followed by oxidation of the central cobalt cation. Alternatively, in the presence of excess CoIILaq, LCoII‐OOSO3aq reacts with CoIILaq to form 2CoIIILaq. These results point out that the mechanism of advanced oxidation processes initiated by a mixture of Co(H2O)62+ and HSO5− must be re‐considered.
Flower‐like NiCo‐carbonate Hydroxides for High‐performance Solid‐state Hybrid Supercapacitor
Authors: Wang Jingruo , Zhang Deyi , She Wenna , Gao Shiyao , Wang Kunjie , Wang Yi , Han Zhiyong , Chen Xuefu , Li Lan 
Published: December 2021

AbstractCompared to the traditional transition metal layered double hydroxides, transition metal layered carbonate double hydroxides (TMC‐LDHs) possess superior electrochemical performance in theory. But TMC‐LDHs have not received its deserved attention, especially for application in the energy storage field. In this work, a flower‐like TMC‐LDH (Ni0.75Co0.25(CO3)0.125(OH)2, NCCO) material was successfully prepared by hydrothermal method, which exhibits a high specific capacity of 306.8 mAh g−1 (0.52 mAh cm−2) at 0.5 A g−1 with capacity retention of 70.5 % after 2000 cycles. The solid‐state hybrid supercapacitor device NCCO//PVA/KOH//IHPC based on the prepared NCCO material and an interconnected hierarchical porous carbon (IHPC) delivers a high specific energy of 50.96 Wh kg−1 at a specific power of 1.06 kW kg−1, and a high specific energy of 36.39 Wh kg−1 still can be delivered at a high specific power of 10.49 kW kg−1. More than 181.2 % of initial specific capacity is retained after 12000 cycles. The specific energy, energy retention under large specific power, and the cycle stability of the assembled device are better than most of the solid‐state hybrid supercapacitors that have been reported. These results demonstrate the promising prospect of the TMC‐LDH material in the practical application in advanced solid‐state supercapacitors.
UHPLC‐QTOF/MS‐based comparative metabolomics in pectoralis major of fast‐ and slow‐growing chickens at market ages
Authors: Zhang Jian , Cao Jing , Geng Ailian , Wang Haihong , Chu Qin , Yan Zhixun , Zhang Xiaoyue , Zhang Yao , Liu Huagui 
Published: December 2021

AbstractThe molecular regulatory mechanism underlying meat quality between different chicken genotypes remains elusive. This study aimed to identify the differences in metabolites and pathways in pectoralis major (breast muscle) between a commercial fast‐growing chicken genotype (Cobb500) and a slow‐growing Chinese native chicken genotype (Beijing‐You chickens, BYC) at market ages respectively based on ultra‐high‐performance liquid chromatography‐quadrupole/time of flight mass spectrometry (UHPLC‐QTOF/MS). Eighteen metabolites were identified as potential biomarkers between BYC and Cobb500 at market ages. Among them, L‐cysteine exhibited a higher relative intensity in BYC compared with Cobb500 and was enriched into 10 potential flavor‐associated KEGG pathways. In addition, the glycerophospholipid metabolism pathway was found to be associated with chicken meat flavor and the accumulation of sn‐glycerol 3‐phosphate and acetylcholine was more predominant in BYC than that in Cobb500, which were catalyzed by glycerophosphocholine phosphodiesterase (GPCPD1, EC:3.1.4.2), choline O‐acetyltransferase (CHAT, EC:2.3.1.6), and acetylcholinesterase (ACHE, EC:3.1.1.7). Overall, the present study provided some metabolites and pathways for further investigating the roles of the differences in meat flavor quality in breast muscle between Cobb500 and BYC at market ages.
Cover Image, Volume 56, Issue 9
Authors: Zhao Xiangdong , Wang Wei , Xie Guwei , Pan Songqi , Jarzembowski Edmund A. , Zheng Daran 
Published: December 2021

The cover image is based on the Research Article Depositional environment of Middle Triassic organic‐rich shales in the Ordos Basin, Northwest China by Wei Wang et al., https://doi.org/10.1002/gj.4215.
image
Dysfunction of cerebellar microglia in Ataxia‐telangiectasia
Authors: Levi Hadar , Bar Ela , Cohen‐Adiv Stav , Sweitat Suzan , Kanner Sivan , Galron Ronit , Mitiagin Yulia , Barzilai Ari 
Published: December 2021

AbstractAtaxia‐telangiectasia (A‐T) is a multisystem autosomal recessive disease caused by mutations in the ATM gene and characterized by cerebellar atrophy, progressive ataxia, immunodeficiency, male and female sterility, radiosensitivity, cancer predisposition, growth retardation, insulin‐resistant diabetes, and premature aging. ATM phosphorylates more than 1500 target proteins, which are involved in cell cycle control, DNA repair, apoptosis, modulation of chromatin structure, and other cytoplasmic as well as mitochondrial processes. In our quest to better understand the mechanisms by which ATM deficiency causes cerebellar degeneration, we hypothesized that specific vulnerabilities of cerebellar microglia underlie the etiology of A‐T. Our hypothesis is based on the recent finding that dysfunction of glial cells affect a variety of process leading to impaired neuronal functionality (Song et al., 2019). Whereas astrocytes and neurons descend from the neural tube, microglia originate from the hematopoietic system, invade the brain at early embryonic stage, and become the innate immune cells of the central nervous system and important participants in development of synaptic plasticity. Here we demonstrate that microglia derived from Atm−/− mouse cerebellum display accelerated cell migration and are severely impaired in phagocytosis, secretion of neurotrophic factors, and mitochondrial activity, suggestive of apoptotic processes. Interestingly, no microglial impairment was detected in Atm‐deficient cerebral cortex, and Atm deficiency had less impact on astroglia than microglia. Collectively, our findings validate the roles of glial cells in cerebellar attrition in A‐T.
Synergistic anticancer effect of a combination of chidamide and etoposide against NK/T cell lymphoma
Authors: Song Wenting , Chen Zhan , Shi Cunzhen , Gao Yuyang , Feng Xiaoyan , Li Hongwen , Li Zhaoming , Zhang Mingzhi 
Published: December 2021

AbstractNatural killer/T cell lymphoma (NKTCL) is a highly aggressive hematological malignancy. However, there is currently no consensus on therapies for refractory/relapsed patients. In this study, we investigated the synergistic anticancer effect and potential mechanism of combining chidamide, a histone deacetylases (HDACs) inhibitor, and etoposide, a DNA‐damaging agent, in NKTCL. We demonstrated that chidamide or etoposide alone dose‐ and time‐dependently inhibited the cell viability of NKTCL cell lines, YT, NKYS and KHYG‐1. Functional experiments suggested that combined chidamide and etoposide treatment exerted synergistic antiproliferation effect and enhanced cell apoptotic death in vitro and in vivo. Furthermore, the expression of DNA damage related proteins was detected and we also examined the alternations in histone acetylation, cell cycle progression, and mitochondrial membrane potential (MMP). The results suggested that increased histone acetylation, cell cycle arrest at the G2/M phase and loss of MMP, converging to greater DNA damage, might account for the synergism of the combination of chidamide and etoposide in NKTCL. Taken together, our study provides an evident for possible application on combining HDACs inhibitors and DNA‐damaging agents for the treatment of NKTCL.
Hepatitis B virus infection and the risk of gastrointestinal cancers among Chinese population: A prospective cohort study
Authors: Liu Tong , Song Chunhua , Zhang Youcheng , Siyin Sarah Tan , Zhang Qi , Song Mengmeng , Cao Liying , Shi Hanping 
Published: December 2021

AbstractOur study aims to explore the relationship between chronic hepatitis B virus (HBV) infection and the risk of gastrointestinal (GI) cancers including liver, gastric, gallbladder or extrahepatic bile duct, pancreatic, small intestine, esophageal and colorectal cancer in the Kailuan Cohort study. We prospectively examined the relationship between HBV infection and new‐onset GI cancers among 93 402 participants. Cox proportional hazards regression models, subgroup analyses and competing risk analyses were used to evaluate the association between HBV infection and the risk of new‐onset GI cancers. During a median follow‐up of 13.02 years, 1791 incident GI cancer cases were diagnosed. Compared to HBsAg seronegative participants, a significant positive association between HBV infection and GI cancers was observed in the multivariate‐adjusted models (HR 5.59, 95% CI: 4.84‐6.45). In the site‐specific analyses, participants with HBsAg seropositive exhibited an increased risk of liver cancer (HR = 21.56, 95% CI: 17.32‐26.85), gallbladder or extrahepatic bile duct cancer (HR = 14.89, 95% CI: 10.36‐21.41), colorectal cancer (HR = 1.75, 95% CI: 1.15‐2.96) and pancreatic cancer (HR = 1.86, 95% CI: 1.10‐3.99). After taking death as the competing risk event, the associations of HBV infection with the risk of these cancers were attenuated but remained significant both in the cause‐specific hazards models, the subdistribution proportional hazards models and sensitivity analyses. Our study suggests that HBV infection is associated with the elevated risk of liver cancer and extrahepatic cancer including gallbladder or extrahepatic bile duct, pancreatic and colorectal cancer among adults in Northern China.
Double renal cell carcinoma with histological type of clear cell carcinoma and papillary carcinoma in the same kidney concurrently treated with robot‐assisted partial nephrectomy
Authors: Shimizu Teruki , Hongo Fumiya , Takahashi Hikaru , Fujihara Atsuko , Ukimura Osamu 
Published: December 2021

IntroductionThe presence of two different histologic types of renal cell carcinoma in the same kidney is rare in clinical practice. This report describes a patient with ipsilateral renal cell carcinomas, consisting of a clear cell renal cell carcinoma and a papillary type 1 renal cell carcinoma, who was successfully treated by robot‐assisted partial nephrectomy.Case presentationA 70‐year man was referred to our hospital for the treatment of two right mid‐pole renal tumors, measuring 51 mm and 31 mm in diameter. The two tumors, which differed in contrast enhancement on computed tomography, were removed simultaneously by robot‐assisted partial nephrectomy. Histopathological and immunohistochemical findings confirmed that one tumor was a clear cell renal cell carcinoma, pT1a, and the other was a papillary type1 renal cell carcinoma, pT1b.ConclusionThis report describes a rare patient presenting with two ipsilateral renal cell carcinomas differing in histology. Robot‐assisted partial nephrectomy was the safe and effective nephron‐sparing surgery, even in patients with complex double renal tumors.