bluuebunny/crossref_metadata_2025_split huggingface dataset:
Novel Mutations in Thiazide-Sensitive Na-Cl Cotransporter Gene of Patients with Gitelman's Syndrome
Authors: MONKAWA TOSHIAKI , KURIHARA ISAO , KOBAYASHI KAZUO , HAYASHI MATSUHIKO , SARUTA TAKAO 
Published: May 2021

Abstract.
Gitelman's syndrome (GS) is an autosomal recessive disorder characterized by metabolic alkalosis, hypokalemia, hypomagnesemia, and hypocalciuria that has recently been reported to be linked to thiazide-sensitive Na-Cl cotransporter (TSC) gene mutations. In this study, possible mutations in the TSC gene of six Japanese patients clinically diagnosed with GS were investigated. Twenty-six exons encoding TSC were amplified by PCR and then completely sequenced by the direct sequencing method. Patient A showed a missense mutation of Arg 642 to Cys on the paternal allele and a missense mutation of Val 578 to Met and a 2-bp deletion (nucleotide 2543-2544) on the maternal allele. This deletion results in a frameshift that alters codon 837 to encode a stop signal rather than phenylalanine, and it is predicted to lead to loss of the latter half of the intracellular carboxy terminus. In the second family, two affected sisters, patients B and C, had a homozygous missense mutation of Thr 180 to Lys. Both of their parents, who are consanguineously married, have a heterozygous Thr180Lys mutation. Patient D has a homozygous mutation Thr180Lys, which is the same as the second family. Haplotype analysis indicates that patients B and C are not related to patient D. In patients E and F, we could identify only one mutant allele; Ala569Glu and Leu849His, respectively. All of the mutations identified are novel except for the Arg642Cys mutation, which has been found in a Japanese GS patient. Although further in vitro study is required to prove that the mutations are responsible for GS, it is possible that Thr180Lys and Arg642Cys mutations might be common mutations in Japanese GS.
Lovastatin Inhibits Transforming Growth Factor-β1 Expression in Diabetic Rat Glomeruli and Cultured Rat Mesangial Cells
Authors: KIM SUNG IL , HAN DONG CHEOL , LEE HI BAHL 
Published: May 2021

Abstract.
Diabetic nephropathy is a leading cause of end-stage renal disease and is characterized by excessive deposition of extracellular matrix (ECM) proteins in the glomeruli. Transforming growth factor-β (TGF-β) is the major mediator of excessive accumulation of ECM proteins in diabetic nephropathy through upregulation of genes encoding ECM proteins as well as downregulation of genes for ECM-degrading enzymes. It has been shown that lovastatin, an inhibitor of 3-hydroxy-3-methylglutaryl CoA reductase, delays the onset and progression of different models of experimental nephropathy. To evaluate the effect of lovastatin on the development and progression of diabetic nephropathy, streptozotocin-induced diabetic rats were studied for 12 mo. In untreated diabetic rats, there were significant increases in blood glucose, urine albumin excretion, kidney weight, glomerular volume, and TGF-β1 mRNA expression in the glomeruli compared with normal control rats treated with citrate buffer only. Treatment with lovastatin in diabetic rats significantly suppressed the increase in urine albumin excretion, kidney weight, glomerular volume, and TGF-β1 mRNA expression despite high blood glucose levels. To elucidate the mechanisms of the renal effects of lovastatin, rat mesangial cells were cultured under control (5.5 mM) or high (30 mM) glucose with lovastatin alone, mevalonate alone, or with both. Under high glucose, TGF-β1 and fibronectin mRNA and proteins were upregulated. These high glucose-induced changes were suppressed by lovastatin (10 μM) and nearly completely restored by mevalonate (100 μM). These results suggest that lovastatin has a direct cellular effect independent of a cholesterol-lowering effect and delays the onset and progression of diabetic nephropathy, at least in part, through suppression of glomerular expression of TGF-β1.
Electrophysiologic Characterization of an Organic Anion Transporter Cloned from Winter Flounder Kidney (fROAT)
Authors: BURCKHARDT BIRGITTA CHRISTINA , WOLFF NATASCHA A. , BURCKHARDT GERHARD 
Published: May 2021

Abstract.
The two-electrode voltage clamp technique was used to demonstrate translocation of p-aminohippurate (PAH) and related compounds such as loop diuretics in Xenopus laevis oocytes expressing the renal organic anion transporter from winter flounder kidney (fROAT). In fROAT-expressing oocytes, PAH (0.1 mM) induced a depolarization of 4.2 ± 0.4 mV and at a holding potential of -60 mV an inward current of -22.6 ± 3.5 nA. PAH-induced current and the current calculated from [3H]-PAH uptake were of similar magnitude. Depolarization, inward current, and current-to-uptake relation indicated exchange of the monovalent PAH with a divalent anion, possibly α-ketoglutarate (α-KG), causing net efflux of one negative charge. The kinetic analysis of PAH-induced currents revealed that translocation is dependent on membrane potential, saturable with an apparent K
m of 58 ± 8 μM, and sensitive to probenecid and furosemide. In contrast to probenecid and furosemide, the loop diuretics bumetanide, ethacrynic acid, and tienilic acid and the nephrotoxic mycotoxin ochratoxin A elicited inward currents indicating translocation through fROAT. Substrate-dependent currents provide a tool to elucidate the structure/function relationship of the renal organic anion transporter.
Test excavation of the “pseudo-ditch” system of the Late Neolithic settlement complex at Öcsöd-Kováshalom on the Great Hungarian Plain
Authors: Füzesi András , Rassmann Knut , Bánffy Eszter , Hoehler-Brockmann Hajo , Kalla Gábor , Szabó Nóra , Szilágyi Márton , Raczky Pál 
Published: May 2021

In 2018, the RGK’s research team led by Knut Rassmann undertook the magnetometer survey of the Late Neolithic site of Öcsöd-Kováshalom over a roughly 65 ha large area. An enclosure system of three concentric semi-circular ditches was detected around the tell-like mound that formed the core of the settlement. In order to resolve these issues, we organised a small field school excavation with the support of the RGK and with the participation of the archaeology students of the ELTE Institute of Archaeological Sciences between July 13 and 25, 2020. We opened an 8×2 m trial trench across the north-eastern part of the innermost ditch appearing on the magnetogram. We uncovered a ditch segment with a V-shaped cross-section, a width of 315 cm and a depth of 295 cm. We recovered a total of 17,430 finds with a weight of 194.4 kg (pottery sherds, daub, animal bones, bone tools, chipped and polished stone implements, quern stones, mussels, and ochre). In our preliminary report, we made a reconstruction of infilling process based on the quantitative distribution of the finds (frequency and weight data) and the different characteristics of the fill layers.
Human remains unearthed in settlement context from the Late Bronze Age – Early Iron Age (Reinecke BD–HaB3) Northeastern Hungary
Authors: Király Ágnes 
Published: May 2021

Abstract of PhD thesis submitted in 2020 to the Archaeology Doctoral Programme, Doctoral School of History, Eötvös Loránd University, Budapest under the supervision of Gábor V. Szabó.
Czajlik, Z. – Črešnar, M. – Doneus, M. – Fera, M. – Hellmith Kramberger, A. – Mele, M. (eds): Researching Archaelogical Landscapes Across Borders – Strategies, Methods and Decisions for the 21th Century. Archaeolingua, Graz–Budapest, 2019.
Authors: Mesterházy Gábor 
Published: May 2021

The reviewed volume is one output of the Interreg DTP Monumentalized Early Iron Age Landscapes in the Danube river basin (acronym Iron-Age-Danube) program, which involved professionals from Austria, Croatia, Hungary, Slovakia and Slovenia.
Hemşirelik Öğrencilerinde Ruhsal Sağlık, Yorgunluk ve Uyku Kalitesi Arasındaki İlişki: Kesitsel Bir Çalışma
Authors: NAZİK Feyza , DİŞLİ Faruk 
Published: May 2021

Amaç: Bu çalışma hemşirelik öğrencilerinin ruhsal sağlık, yorgunlukve uyku kaliteleri arasındaki ilişkiyi ve etkileyen faktörleri belirlemek amacı ile yapılmıştır.
Gereç ve Yöntemler: Çalışma tanımlayıcı ve kesitsel olarak yapılmıştır. Çalışmanın evreni 247 hemşirelik öğrencisidir. Örneklem 230 öğrencidir. Verilerin toplanmasında sosyo-demografik özellikler, Genel Sağlık Anketi, Yorgunluk Şiddet Ölçeği ve ve Pittsburgh Uyku Kalitesi İndeksi kullanılmıştır. Verilerin
analizinde Mann-Whitney U testi, Kruskal-Wallis ve Spearman korelasyon analizi kullanılmıştır.
Bulgular: Öğrencilerin yaş ortalamaları 21.4±3.6 idi. Yüzde 9.6’sının kronik hastalığı bulunmaktaydı. Hiç sigara içmeyen öğrenci oranı %52.6; uyku düzeni olmayan öğrenci oranı ise % 52.2 idi. Kronik hastalığı olan ve düzensiz uykusu olan öğrencilerin genel sağlık ölçeği puan ortalaması anlamlı olarak yüksekti
(p
Modern aspects of targeted prostate biopsy
Authors: Popov S. V. , Orlov I. N. , Malevich S. M. , Sushina I. V. , Grin’ E. A. , Gulko A. M. , Topuzov T. M. , Vyazovtsev P. V. , Chernysheva D. Yu. , Neradovskiy V. A. 
Published: May 2021

Prostate cancer (PCa) is in second place in oncological morbidity in males and is the fifth leading cause of death among the world's population. According to current world statistics, over the past 20 years there has been an increase in primary morbidity, as well as mortality from PCa. The key to diagnosing PCa is a prostate biopsy. Nevertheless, a systemic biopsy under transrectal ultrasound control is the subject of discussion and debate in oncourology, as it has significant drawbacks that affect the qualitative result of the diagnosis of PCa. Given the importance of adequate and staged PCa, various methods of targeted biopsy under magnetic resonance control have been proposed.This review will examine the main features and significance of targeted prostate biopsy, as well as the role of magnetic resonance imaging in the early diagnosis of PCa. The method of targeted biopsy of the prostate gland can improve the detection of PCa in relation to clinically significant forms. In addition, this method is extremely promising and requires further study to further improve the quality of early diagnosis of PCa, especially when selecting patients for radical surgical treatment.
An original surgical method for the formation of fascial duplication in the elimination of damage to the anterior rectal wall during prostatectomy
Authors: Yurgel Yu. N. , Alekseev B. Ya. , Kopyltsov E. I. , Leonov O. V. , Sikhvardt I. A. , Leonova A. O. , Vodolazskiy V. A. , Durnev I. A. , Yurgel N. Yu. 
Published: May 2021

Background. Intraoperative rectal injury in patients undergoing prostatectomy is a severe complication, worsens the quality of life and leads to social maladjustment.Objective. To develop, substantiate the reproducibility and safety of the technique for the formation of fascial duplication in the elimination of damage to the anterior rectal wall during prostatectomy.Materials and methods. The authors present a description of the technique and their own experience of retropubic prostatectomy with the formation of fascial duplication in the elimination of damage to the anterior rectal wall in 2 patients with prostate cancer. Patients with intermediate risk of recurrence and progression according to the D'Amico index. The observation period is 6 and 11 months.Results. Prostatectomy revealed damage to the anterior rectal wall and destruction of the external longitudinal muscular layer of the rectum. The rectal defect was eliminated according to the original method. The duration of the operation is 160 and 140 min. Intraoperative blood loss 350 and 150 ml. The first bowel movement on the 5th day. Removal of the urethral catheter on day 10. The period of hospitalization was 11 and 14 days, no postoperative mortality was noted. No lethal outcomes were noted.Conclusion. A constructively simple surgical technique for the formation of fascial duplication by local tissues in case of intraoperative injury of the rectum during prostatectomy has been developed and implemented into clinical practice.
<i>FGFR3, TERT, ТР53</i> mutations and the <i>FGFR3</i> gene expression in bladder cancer as prognostic markers
Authors: Mikhaylenko D. S. , Sergienko S. A. , Kuznetsova E. B. , Zaborsky I. N. , Martynov M. I. , Loran O. B. , Efremov G. D. , Samoylova S. A. , Alekseev B. Ya. , Musatova V. V. , Bure I. V. , Nemtsova M. V. 
Published: May 2021

Background. Bladder cancer (BC) is a common urological cancer, 75 % of which are non-muscle invasive BC. After removal of the primary tumor, the adequate classification of malignancy and the defining of tumor progression risk remains an important issue, since it is associated with frequency of cystoscopy and choice of the BCG- or chemotherapy management.Objective: improve the algorithms of prognosis in intermediate-risk patients with non-muscle-invasive bladder cancer with the consideration of molecular characteristics of the primary tumor.Materials and methods. We studied 125 BC samples; mutations in the FGFR3, PIK3CA, TERT, and TP53 genes were determined by polymerase chain reaction and Sanger sequencing, as well as the expression of the FGFR3, EGFR, ERBB2, FOXA1, and GATA3 genes using realtime polymerase chain reaction.Results. Somatic mutations in the studied loci were detected in 65.6 % of the samples, five new mutations were identified. A decrease of the mutation frequency in the FGFR3 and TERT genes was shown, an increase — TP53 in order (Ta—T1/low G) > (T1/high G) > (>T2/any G). The largest area under the ROC curve (0.807 ± 0.092, р = 0.004) was demonstratedfor the prognostic classifier with the independent variables: mutation in FGFR3 and/or TERT; mutation TP53; overexpression of the FGFR3 gene. The FGFR3, TERT mutations and/or FGFR3 overexpression in the absence of TP53 mutation indicates minimally invasive primary tumor. On the contrary, harboring TP53 mutation indicate the features of muscle-invasive BC at the genetic level. Using this algorithm, we reclassified 21 of T1G3 BC cases as having characteristics associated with non-invasive tumor in 43 %, and invasive BC in 57 % of patients.Conclusion. The aforementioned prognostic model could be used as additional laboratory test in assessing the malignancy and progression risk of non-muscle invasive BC.
THE PROBLEM OF HALITOSIS IN PERIODONTAL COMPLEX DIAGNOSTICS AND TREATMENT. LITERATURE REVIEW
Authors: Ssablina Svetlana , Elovicova Tat'yana , Griroriev Sergey , Yepishova Anna , Bushueva Elizaveta , Andreeva Anna , Shimova Margarita , Sorokoumova Dina , Chagay Andrey 
Published: May 2021

Subject. Oral malodour (halitosis) in patients with periodontal disease.
Objectives. To study and review the literature on local (dental) and general (internal diseases) factors causing halitosis in patients with periodontal disease.
Conclusion. The present literature review provides a sufficient basis to state that the problem of halitosis is relevant; there is presented the overview of developed classifications and identified combinations of main causes of halitosis linking the extent of ozostomia to oral hygiene status and gingival hemorrhage. By modern molecular genetic technologies (polymerase chain reaction and 16S ribosomal RNA gene chipping), advances have been made in understanding the main role of uncultivated anaerobic oral pathogens associated with halitosis. The materials of this literature review help to get the complex idea of the problem of halitosis in patients with inflammatory periodontal disease and can be used by dental clinicians in health care practice.
ASSESSMENT OF THE QUALITY OF LIFE OF PATIENTS AFTER DENTAL INTERVENTIONS
Authors: Balin Kirill , Borisova Eleonora , Fedichkina Marina 
Published: May 2021

Subject. Today, the condition of patients with dental diseases is directly related to the quality of life. The massive need for dental rehabilitation of patients, restoration of chewing function, aesthetic function and normalization of speech requires a full and high-quality treatment result. According to the definition of the World Health Organization, the quality of life is “an individual's perception of their position in life in the context of the culture and value systems in which they live and in relation to their goals, expectations, standards and concerns” [25, 39]. There are two sides of the "quality of life" — internal, which depends on the very personality of a person, and external, due to the degree of development of society and the state. Therefore, the study of the quality of life is not only a reliable and effective way of assessing the general well-being of a person, but also a highly informative, sensitive and economically justified method of assessing the health of both the population as a whole and individual social groups generally accepted in international practice. The techniques described in this review allow one to quantify the multicomponent characteristics of a person's vital activity — his physical, psychological and social functioning. Aim. Analysis of the degree of elaboration of the topic “studying the quality of life”.
Materials and methods. The study was conducted based on the search and study of original articles on aspects of the quality of life of patients after dental interventions. In the course of studying the literature in the eLibrary, Pubmed, Google Schoolar databases, 39 sources were included in the study.
Conclusions. As a result of the work carried out, it can be concluded that the dynamic assessment of the quality of life of patients in the process of dental treatment is a rather promising direction. Subjective assessment of the quality of life by the patient himself is one of the most important criteria for quality treatment.
Educação de jovens e adultos e o planejamento de carreira no CESEC Divinópolis MG
Authors: Farinha Corina Alves , Souza Tânia Nogueira Fonseca 
Published: May 2021

Trata-se do resultado de um Projeto de Extensão, tutelado pela Universidade do Estado de Minas Gerais – UEMG, Divinópolis - MG, Edital PROINPE 1/2019, realizado no Centro Estadual de Educação Continuada - CESEC Dr. Fábio Botelho Notini, Divinópolis – MG. Objetivou-se sensibilizar um grupo de jovens e adultos para a importância de estruturar um projeto de vida profissional, elaborando plano de carreira. Aplicou-se um questionário para conhecer o público alvo. Os dados de 69 estudantes de EJA indicam: 54% adultos, de 25 a 60 anos; 46% jovens, 15 a 24 anos; 51% gênero feminino, 51% brancos, 54% não trabalham; 37% não concluíram os estudos no tempo estabelecido pelo MEC em virtude da atividade laboral e família, e 35% por desinteresse; 41% escolheu a formação ágil do CESEC, 58% retomaram os estudos para capacitar-se; e após o CESEC 51% desejam graduar-se. Considera-se impacto na sociedade a possibilidade de contribuir para a reflexão e construção do plano de carreira de sujeitos cuja trajetória de vida pessoal e profissional abarca saberes diversos. Os estudantes do CESEC explicitaram a importância da atividade para constituir o futuro profissional; diretor, docentes e funcionários consideraram importante a atuação da Universidade pública no ambiente escolar do CESEC levando os estudantes de EJA a refletir sobre estudo, trabalho e profissão. Os graduandos relataram a relevância de conhecer e analisar a trajetória de vida pessoal e profissional de sujeitos que se esforçam para retomar os estudos, o entusiasmo e a valorização da educação para conquistar um emprego e exercer a cidadania.
Do processo de formação da legislação cooperativa brasileira à atualidade das cooperativas agrícolas e de consumo: a nova estrutura do cooperativismo brasileiro
Authors: Corrêa Lima de Miranda Victória , De Miranda José Eduardo 
Published: May 2021

O presente trabalho aborda o processo histórico de formação da legislação cooperativa brasileira, perpassando pela expressão do Cooperativismo no plano da Constituição da República Federativa do Brasil, de 1988. Assim mesmo, e tendo em vista sua importância para o desenvolvimento socioeconômico da sociedade brasileira, retrata as principais características dos ramos agropecuário e de consumo. Finalmente, e levando em consideração a atuação da Organização das Cooperativas Brasileiras, apresenta a atual estruturação dos ramos cooperativos.
Recibido: 08 febrero 2021Aceptado: 10 marzo 2021
Incidência de normas concorrenciais em cooperativas: a intervenção estatal sobre Cooperativas no Brasil
Authors: Borges Alves Giselle 
Published: May 2021

As atividades econômicas desenvolvidas pelas cooperativas brasileiras durante décadas têm despertado a atuação do Conselho Administrativo de Defesa Econômica (CADE), tendo em vista que a garantia constitucional de não intervenção estatal (art. 5.º, XVIII) não pressupõe imunidade à aplicação da legislação antitruste. Diante deste enfoque, a pesquisa teve como objetivo analisar algumas das decisões do CADE sobre as sociedades cooperativas, bem como estabelecer a necessidade de reflexão sobre as características e peculiaridades distintivas das sociedades cooperativas que justificam a coordenação de agentes no mercado.
Recibido: 09 noviembre 2020Aceptado: 14 enero 2021
Critical design method as an approach to improve design thinking among Pearl Academy students
Authors: Sugathan Manju 
Published: May 2021

Over the course of time the designers have mastered the skill that can be applied to resolve a wide range of problems through analysis of a hypothesis using different research methods strategically and iteratively. The task ahead for the new generation designers is to develop designs that are preferable for a broader diverse population who needs personal understanding of their requirements. Critical design thinking could be applied as an approach to favour the designers to focus on future requirements of people and see beyond their ideological constrains in design.
Critical design favours the development of critical thinking that supports the personal understanding of a design idea or proposal through expanding the meaning of design thinking. It can be adapted as a method challenging and improving the field of design, in order to achieve best teaching and learning in universities and design colleges. The purpose of the study is to test: by employing critical design method in design education to help the students learn, analyse and evaluate ideas and concepts in a critical manner. Also, it examines critical design as a method for questioning and improving design thinking among students that synergises perfectly with future design thinkers as well as their potential audience.
A critical design workshop is conducted in Pearl Academy, Mumbai by the author to test and develop this way of thinking among design students. The workshop results are analysed under Bloom’s taxonomy, which is a set of three hierarchical models used to classify educational learning objectives under analysis, synthesis and evaluation (Torkildsby, 2018). An advantage of critical design, is that the method allows students to temporarily move away from problem solving, focusing on asking questions instead of providing answers – finding problems rather than solving them (Dunne & Raby, 2013, p.7).
Chrześcijaństwo: powstanie i upadek mitu. Na marginesie książki Tomasza Polaka System kościelny
Authors: Ziemiński Ireneusz 
Published: May 2021

The paper is a discussion of Tomasz Polak’s book System kościelny, czyli przewagi pana K. [The Church System, or the Dominance of Mr. K.]. The author emphasizes the signiﬁcance of Polak’s book not only for religious studies, given especially his original thesis about Christianity’s atheistic core, but also for other ﬁelds within humanities, dealing with the origins, development and fall of non-religious myths, social or national.