bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Single Amino Acid Change In Histone Methyltransferase CURLY LEAF Results In Premature Bolting In Chinese Cabbage (Brassica Rapa L. Ssp. Pekinensis)
Authors: Tan Chong , Ren Jie , Wang Lin , Ye Xueling , Fu Wei , Zhang Jiamei , Qi Meng , Feng Hui , Liu Zhiyong 
Published: January 2021

Abstract
Background: Flowering is an important inflection point in the transformation from vegetative to reproductive growth, and premature bolting severely decreases crop yield and quality. Results: In this study, a stable early-bolting mutant, ebm3, was identified in an ethyl methanesulfonate (EMS)-mutagenized population of a Chinese cabbage doubled haploid (DH) line ‘FT’. Compared with ‘FT’, ebm3 showed early bolting under natural cultivation in autumn, and curled leaves. Genetic analysis showed that the early-bolting phenotype was controlled by a single recessive nuclear gene. Modified MutMap and genotyping analyses revealed that Brebm3 (BraA04g017190.3C), encoding the histone methyltransferase CURLY LEAF (CLF), was the causal gene of the emb3. A C to T base substitution in the 14th exon of Brebm3 resulted in an amino acid change (S to F) and the early-bolting phenotype of emb3. The mutation occurred in the SET domain (Suppressor of protein-effect variegation 3-9, Enhancer-of-zeste, Trithorax), which catalyzes site- and state-specific lysine methylation in histones. Tissue-specific expression analysis showed that Brebm3 was highly expressed in the flower and bud. Promoter activity assay confirmed that Brebm3 promoter was active in inflorescences. Subcellular localization analysis revealed that Brebm3 localized in the nucleus. Transcriptomic studies supported that Brebm3 mutation might repress H3K27me3 deposition and activate expression of the AGAMOUS (AG) and AGAMOUS-like (AGL) loci, resulting in early flowering.Conclusions: Our study revealed that an EMS-induced early-bolting mutant ebm3 in Chinese cabbage was caused by a nonsynonymous mutation in BraA04g017190.3C, encoding the histone methyltransferase CLF.These results improve our knowledge of the genetic and genomic resources of bolting and flowering, and may be beneficial to the genetic improvement of Chinese cabbage.
Cryo-EM structure of human Wntless in complex with Wnt3a
Authors: Zhong Qing , Ye Fangfei , Xiao Zaiyu , Zhang Yuanyuan , Huang Gaoxingyu , Zhan Xiechao , Sun Ke , Wang Zhizhi , Cheng Shanshan , Feng Shan , Xu Meng , Zhao Xiuxiu , Zhang Jizhong , Lu Peilong , Xu Wenqing , Zhou Qiang , Ma Dan 
Published: January 2021

Abstract
Wntless (WLS), an evolutionarily conserved multi-pass transmembrane protein, is essential for secretion of Wnt proteins. Wnt-triggered signaling pathways control many crucial life events, whereas aberrant Wnt signaling is tightly associated with many human diseases including cancers. Here, we report the cryo-EM structure of human WLS in complex with Wnt3a, the most widely studied Wnt, at 3.8 Å resolution. The transmembrane domain of WLS bears a GPCR fold, with a conserved core cavity and lateral opening. A β-hairpin of Wnt3a containing a conserved palmitoleoylation site has extensive interactions with the WLS luminal domain, and the tip region is accommodated in the WLS cavity. The flexibility of Wnt3a loop/hairpin regions involved in the multiple binding sites indicates a possible sequential release mechanism when Wnts are transferred to their receptors. We found Wnt3a palmitoleoylation site mutant has similar binding capability with WLS compared with wild type Wnt3a, suggesting palmitoleoylation is not essential for Wnt-WLS association. Our findings provide important insights into the molecular mechanism of Wnt palmitoleoylation, secretion and signaling.
Modified π-shaped Esophagojejunostomy in Totally Laparoscopic Total Gastrectomy: a Report of 40 Consecutive Cases From a Single Center
Authors: Xing Jiadi , Xu Kai , Liu Maoxing , Gao Pin , Tan Fei , Yao Zhendan , Zhang Nan , Yang Hong , Zhang Chenghai , Cui Ming , Su Xiangqian 
Published: January 2021

Abstract
Background: Intracorporeal esophagojejunostomy remains a challenging technique in totally laparoscopic total gastrectomy (TLTG) without established standard anastomosis method. π-shaped esophagojejunostomy in TLTG was reported to be safe and feasible. Therefore, this study aimed to depict our modified π-shaped esophagojejunostomy in TLTG and evaluate its short-term surgical outcomes.Methods: From April 2018 to October 2019, 40 patients with gastric cancer who underwent TLTG by the same surgeon with modified π-shaped esophagojejunostomy were enrolled. Clinicopathologic data including age, gender, body mass index, American Society of Anesthesiologists score, tumor size, tumor location, depth of tumor invasion, number of harvested lymph nodes, nodal metastasis and TNM stage, operative time, estimated blood loss, time to resume diet, postoperative hospital stays, complication, and mortality were collected and retrospectively analyzed.Results: The mean operative time and estimated blood loss were 264.6 ± 56.9 min and 68.5 ± 53.3 mL, respectively. Postoperative flatus occurred at 4.6 ± 1.7 days. The mean time to resume diet was 7.4 ± 1.7 days postoperatively. One patient was diagnosed with anastomotic leakage and managed with conservative therapy. Pleural effusion, occurring in 4 (10%) patients, was the most common complication. One patient experienced intra-abdominal bleeding requiring reoperation. Other complications were atrial fibrillation and wound infection. No mortality occurred during the 6-month follow-up.Conclusions: Modified π-shaped esophagojejunostomy is a safe and feasible method for intracorporeal anastomosis in TLTG, which showed favorable surgical outcomes.
Quantifying Finer-scale Behaviours Using Self-organising Maps (SOMs) to Link Accelerometery Signatures With Behavioural Patterns in Free-roaming Terrestrial Animals.
Authors: Galea Nicole , Murphy Fern , Gaschk Joshua , Schoeman David , Clemente Christofer 
Published: January 2021

Abstract
Collecting quantitative information on animal behaviours is difficult, especially from cryptic species or species that alter natural behaviours in the presence of humans. We develop a methodology that can precisely classify finer-scale behaviours in free roaming animals. We deployed harness-mounted tri axial accelerometers on 10 free-roaming domestic cats (Felis Catus) over eight days. We also tested two treatments using a prey protector device designed to reduce prey capture.We aligned accelerometer traces collected at 50Hz with video files (60 fps) and labelled 12 individual behaviours, then trained a supervised machine-learning algorithm using Kohonen super Self-Organising Maps (SOM). The SOM was able to predict individual behaviours with a ~ 99% overall accuracy. There was a significant effect of sample size, with precision and sensitivity decreasing rapidly below 2,000 1-second observations. We were also able to detect a behaviour specific reduction in the predictability when cats were fitted with the prey-protector device indicating the it alters biomechanical gait.Our results can be applied in movement ecology, zoology and conservation, where habitat specific movement performance between predators or prey may be critical to managing species of conservation significance, or in veterinary and agricultural fields, where early detection of movement pathologies can improve animal welfare.
Deciphering spatial genomic heterogeneity at a single cell resolution in multiple myeloma
Authors: Merz Maximilian , Merz Almuth , Wang Jie , Wei Lei , Hu Qiang , Hutson Nicholas , Rondeau Cherie , Celotto Kimberly , Belal Ahmed , Alberico Ronald , Block AnneMarie , Mohammadpour Hemn , Wallace Paul , Tario Joseph , Luce Jesse , Glenn Sean , Singh Prashant K. , Herr Megan , Hahn Theresa , Samur Mehmet , Munshi Nikhil , Liu Song , McCarthy Philip , Hillengass Jens 
Published: January 2021

Abstract
Osteolytic lesions (OL) characterize symptomatic multiple myeloma. The mechanisms of how malignant plasma cells (PC) cause OL in one region while others show no signs of bone destruction despite subtotal infiltration remain unknown. We report the first single-cell RNA sequencing (scRNA-seq) study of PC obtained prospectively from random bone marrow aspirates (BM) and paired imaging-guided biopsies of OL. We analyzed 148,630 PC from 24 different locations in 10 patients and observed vast inter- and intra-patient heterogeneity based on scRNA-seq analyses. Beyond the limited evidence for spatial heterogeneity from whole-exome sequencing, we found an additional layer of complexity by integrated analysis of anchored scRNA-seq datasets from the BM and OL. PC from OL were characterized by differentially expressed genes compared to PC from BM, including upregulation of genes associated with myeloma bone disease like DKK1, HGF and TIMP-1 as well as recurrent downregulation of JUN/FOS, DUSP1 and HBB. Assessment of PC from longitudinally collected samples revealed transcriptional changes after induction therapy. Our study, based on the largest number of PC analyzed by scRNA-seq, contributes to the understanding of destructive myeloma bone disease.
Effect of Antenatal Dexamethasone on Respiratory Distress in Late Preterm Infants: Results from the First Vietnamese Matched Cohort Study
Authors: Ho Tran Tuan Hung , Truong Quang Vinh , Nguyen Thi Kim Anh , Le Minh Tam , Nguyen Vu Quoc Huy 
Published: January 2021

Abstract
BackgroundRespiratory distress (RD) is one of the leading causes of early neonatal morbidity and mortality in late preterm infants (LPIs) worldwide. This study aimed to evaluate the effect of antenatal dexamethasone on the RD rate in preterm newborns between 34 0/7 weeks and 36 6/7 weeks of gestation.MethodsThis was a prospective cohort study of 78 women with singleton pregnancy who were in threatened preterm birth and had not received prior dexamethasone who were admitted between 34 0/7 weeks and 36 6/7 weeks at Hue University of Medicine and Pharmacy Hospital from June 2018 to May 2020. The matched control group (no dexamethasone) included 78 pregnant women with threatened late preterm births who were at similar gestational ages and estimated fetal weights as the treatment group. The treatment group received 6 mg intramuscular dexamethasone every 12 hours for a total of 4 doses or until delivery. Primary outcome was the rate of neonatal RD. Secondary neonatal outcomes included the need for respiratory support, neonatal intensive care unit (NICU) admission, hypoglycemia, necrotizing enterocolitis, intraventricular hemorrhage, and neonatal death. Statistical analyses were performed by using SPSS software.ResultsThe rate of RD in LPI was significantly lower in the treatment group than in the matched control group (10.3% vs. 23.1%, respectively), with relative risk [RR] 0.44; 95% confidence interval [CI] 0.21 – 0.96 and p = 0.03. Neonatal hypoglycemia was more common in the dexamethasone group than in the matched group (25.6% vs. 12.8%, respectively; RR, 2.00; 95% CI, 1.00 – 3.99; p = 0.04). There were no significant between-group differences in the incidence of respiratory support, NICU admission or length of hospital stay.ConclusionsAdministration of antenatal dexamethasone to women at risk for late preterm birth could significantly reduce the rate of respiratory distress in late preterm infants.
Räume erschliessen mit einem Smartphone im Sachunterricht an einer Grundschule
Authors: Nickel Stefanie 
Published: January 2021

Schulische Akteurinnen und Akteure, wie z. B. Lehramtsstudierende, haben häufig keine bzw. kaum frühkindliche Erfahrungen mit digitalen Medien, treffen jedoch auf eine nachwachsende Generation von Schülerinnen und Schülern, die bereits von klein auf in irgendeiner Weise in deren Nutzung eingebunden sind und entsprechendes Knowhow mitbringen. Lehr- und Lernprozesse beruhen auf dialogischer Gegenseitigkeit im Sinne einer Verschränkung von Subjekt und Struktur; es gilt daher, einen kooperativen und kommunikativen Erfahrungsraum beim Einsatz digitaler Medien zu schaffen. Um beispielsweise Räume mobil zu erschliessen, bietet sich das Erstellen einer Rallye auf dem Smartphone an. Auf diese Weise wird ein gemeinsamer Sachgegenstand aus dem Lebensumfeld der Schülerinnen und Schüler sowie der Studierenden aufgegriffen. Im Sinne des ausserschulischen Lernens erkunden und erschliessen sich die beteiligten Akteurinnen und Akteure gemeinsam im Team einen bestimmten Ort, um im Anschluss eine Rallye zu planen, auszuarbeiten sowie zu reflektieren. Im Fokus stehen dabei die Anbahnung und der Aufbau von Medienkompetenz durch den Umgang mit digitalen Medien, während im Arbeitsprozess durch dialogisch orientierte Aushandlungsprozesse in Kleingruppen heterogene Medienerfahrungen eingebracht werden. Zu fragen ist daher: Wie kann ein dialogisch angelegter Erfahrungsraum beim mobilen Lernen aussehen?
Occurrence and molecular characterization of Meloidogyne graminicola on rice in Central Punjab, Pakistan
Authors: Jabbar Abdul , Javed Nazir , Munir Anjum , Abbas Huma , Khan Sajid A. , Moosa Anam , Jabran Muhammad , Adams Byron J. , Ali Muhammad A. 
Published: January 2021

Abstract

Meloidogyne graminicola threatens global rice production, yet is understudied for many areas where it is cultivated. To better understand the prevalence and incidence of M. graminicola in central Punjab, Pakistan, we carried out field surveys of rice fields in the districts of Faisalabad and Chiniot. M. graminicola isolates were recovered from soil and root samples and identified on the basis of perineal patterns and rDNA ITS-based sequencing. The severity of nematode attack on rice roots and infested fields at various locations was based on galling index, root-knot nematode juveniles per root system, juveniles per 100 ml of soil, and prevalence of stylet-bearing nematodes and non-stylet-bearing nematodes. Maximum prevalence (22.5 and 27.5%) and minimum prevalence (17.5 and 20%) of M. graminicola was observed in Chiniot and Faisalabad, respectively. Eleven alternate host-plant species were examined in this study revealing varying degrees of M. graminicola infestation. ITS sequencing and phylogenetic analysis indicated that isolates from this study form a well-resolved clade with others from Asia, while another isolate falls outside of this clade in an unresolved polytomy with those from Europe and South America. Though monophyletic with the other M. graminicola, the isolates from Pakistan are distinguished by their high genetic variability and long branch lengths relative to the other isolates of M. graminicola, suggesting Pakistan as a possible ancestral area. Our results indicate that rice is severely attacked by a genetically diverse and aggressive M. graminicola, necessitating the development of appropriate control measures for its management in rice and other graminaceous crops.
The Teaching of Science and Mathematics in English in Malaysia: Teachers’ views on the implementation of two national initiatives
Authors: Ahmad Fuad Nurul Sofia , Abdullah Nabilah , Abdul Rahman Shireena Basree 
Published: January 2021

The Education Ministry has introduced two initiatives - English in the Teaching of Mathematics and Science (ETeMS) and Dual Language Programme (DLP) - to enhance mathematics and science learning using English as the medium of instruction. Numerous discontentment on ETeMS were reported but were not addressed before the DLP introduction in 2016. This qualitative research narrates teachers’ views on both ETeMS and DLP, focusing on the coordinators’ and implementers’ challenges. Eleven mathematics and science school teachers have participated in this case study. Findings reveal that student-, resource- and teacher-related factors remained key reasons impeding effective learning of the two subjects.
Keywords: national policy; teaching and learning; science and mathematics; English.
eISSN: 2398-4287© 2020. The Authors. Published for AMER ABRA cE-Bs by e-International Publishing House, Ltd., UK. This is an open access article under the CC BYNC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). Peer–review under responsibility of AMER (Association of Malaysian Environment-Behaviour Researchers), ABRA (Association of Behavioural Researchers on Asians) and cE-Bs (Centre for Environment-Behaviour Studies), Faculty of Architecture, Planning & Surveying, Universiti Teknologi MARA, Malaysia.
DOI: https://doi.org/10.21834/ebpj.v5iSI3.2554
Postgraduate Students’ Perception on their Studies and Supervisors
Authors: Teoh Sian Hoon , Narayanan Geethanjali , Sidhu Gurnam Kaur , Muthukrishnan Priyadarshini 
Published: January 2021

Postgraduate candidates need to graduate on time (GOT). Failure to GOT among postgraduates is a loss to the nation. GOT should be analyzed from the postgraduate candidates’ perspectives. Therefore, this paper explores postgraduate students’ perspectives about their postgraduate studies. An adapted questionnaire survey was utilized to delve into the postgraduates’ perspective. This quantitative study involved 66 postgraduate candidates from public institutions of higher learning in Malaysia. Initial findings revealed that the candidates rated their supervisor at a higher rating compared to the skills they possessed. Besides, the correlation between critical reading skills and conceptual with research skills is moderately high.
Keywords: postgraduate study, perceptions, skills, supervisory factor, completion of the thesis.
eISSN: 2398-4287© 2020. The Authors. Published for AMER ABRA cE-Bs by e-International Publishing House, Ltd., UK. This is an open access article under the CC BYNC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). Peer–review under responsibility of AMER (Association of Malaysian Environment-Behaviour Researchers), ABRA (Association of Behavioural Researchers on Asians) and cE-Bs (Centre for Environment-Behaviour Studies), Faculty of Architecture, Planning & Surveying, Universiti Teknologi MARA, Malaysia.
DOI: https://doi.org/10.21834/ebpj.v5iSI3.2557
Factors that Discourage Secondary School Students to Enrol in the Civil Engineering Programs
Authors: Mohd Nasir Siti Rashidah , Bariman Mohamad Shahrizan 
Published: January 2021

Enrolment of students in Malaysia tertiary level programs related to STEM-based has shown a significant reduction in numbers which resulted in a decreasing number of enrolment in the Degree of Civil Engineering programs. The purpose of this study is to identify the level of student’s awareness; factors that influence the students; and suggestions for improvement. The respondents are students from secondary school in Negeri Sembilan and UiTM. The findings of this study indicate that there is a significant difference in the level of awareness according to areas; misled perception towards the program; and lack of participation from school and parent.
Keywords: STEM-based education, Civil Engineering, awareness, secondary-level students
eISSN: 2398-4287© 2020. The Authors. Published for AMER ABRA cE-Bs by e-International Publishing House, Ltd., UK. This is an open access article under the CC BYNC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). Peer–review under responsibility of AMER (Association of Malaysian Environment-Behaviour Researchers), ABRA (Association of Behavioural Researchers on Asians) and cE-Bs (Centre for Environment-Behaviour Studies), Faculty of Architecture, Planning & Surveying, Universiti Teknologi MARA, Malaysia.
DOI: https://doi.org/10.21834/ebpj.v5iSI3.2558
Factors Influencing Students' Performance in the First Computer Programming Course Taught Using Blended Learning Approach
Authors: Mabni Zulaile , Shamsudin Noratikah , Aliman Sharifah , Abdul Latif Rosmah 
Published: January 2021

This study investigates the factors that influence the students’ performance in the first computer programming course taught using the blended learning approach. A blended learning model proposed by Hadjerrouit was adapted, which consists of conceptualization, construction, and dialogue phases. The objective of this study is to identify which phase in the adapted blended learning model has an influence on the performance of the students. A cross-sectional population study with simple random sampling was conducted at Universiti Teknologi MARA (UiTM). Stepwise multiple linear regression revealed that only the construction phase of the blended learning model was significant towards students’ performance.
Keywords: Blended Learning; Face-to-Face Learning; Programming; Online Learning
eISSN: 2398-4287© 2020. The Authors. Published for AMER ABRA cE-Bs by e-International Publishing House, Ltd., UK. This is an open access article under the CC BYNC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). Peer–review under responsibility of AMER (Association of Malaysian Environment-Behaviour Researchers), ABRA (Association of Behavioural Researchers on Asians) and cE-Bs (Centre for Environment-Behaviour Studies), Faculty of Architecture, Planning & Surveying, Universiti Teknologi MARA, Malaysia.
DOI: https://doi.org/10.21834/ebpj.v5iSI3.2559
The Politic of Health Communication Model to Improve Government Hospital Service
Authors: Wahid Umaimah 
Published: January 2021

The report aims to investigate poor health services among patients, doctors, medical personnel, non-transparent health information, and unequal health access for the community due to social, political, and economic status. The research used qualitative descriptive methods. The result explained that serious efforts are required to improve health services to the public in hospitals, good relations between doctors, media personnel, and patients. Health communication must be applied to develop good relationships between hospitals, doctors, and patients. Aceh government requires to make a political policy that guarantees the quality of health services equitably and equally. The community has the right to have access to health easily, equitably, and transparently.
Keywords: politic-health communication, model, hospital-government
eISSN: 2398-4287© 2020. The Authors. Published for AMER ABRA cE-Bsby e-International Publishing House, Ltd., UK. This is an open access article under the CC BYNC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). Peer–review under responsibility of AMER (Association of Malaysian Environment-Behaviour Researchers), ABRA (Association of Behavioural Researchers on Asians) and cE-Bs (Centre for Environment-Behaviour Studies), Faculty of Architecture, Planning & Surveying, Universiti Teknologi MARA, Malaysia.
DOI: https://doi.org/10.21834/ebpj.v5iSI3.2563