bluuebunny/crossref_metadata_2025_split huggingface dataset:
Association Between Self-Reported Kinesiophobia and Single-Leg Hop for Distance in Patients With ACL Reconstruction: A Systematic Review
Authors: Bakhsh Hadeel R. , Metikala Sreenivasulu , Billy Gregory G. , Vairo Giampietro L. 
Published: October 2021

Context: Psychological readiness is a significant factor in determining successful return to sport (RTS) and physical activities after anterior cruciate ligament (ACL) reconstruction. Knowing the influence of kinesiophobia on physical tests that are used to guide RTS, such as the single-leg hop for distance (SLHD), would contribute to advancing clinical practice. Objective: To investigate the association between kinesiophobia and SLHD performance in patients after ACL reconstruction. Data Sources: A comprehensive search strategy entailed surveying 6 databases for relevant articles published from January 2009 to March 2021. Study Selection: Articles published in English that were a minimum of level 3 evidence describing kinesiophobia, as measured by the Tampa Scale for Kinesiophobia, and related to SLHD performance in patients after ACL reconstruction. Study Design: Systematic review. Level of Evidence: Level 3. Data Extraction: Study characteristics, sample population demographics, instrument(s), or approach(s) used to assess kinesiophobia and SLHD performance, and corresponding results. Results: A total of 152 potential studies were identified, 106 studies underwent screening, 40 were reviewed in full, and 7 studies were included. Meta-analysis could not be performed because of differences in experimental design among studies and instances of missing outcome data. Currently, moderate evidence indicates patients with ACL reconstruction that exhibit less kinesiophobia perform better on the SLHD test. Conclusion: The outcomes of this review propose that sports health practitioners consider the influence of kinesiophobia on SLHD performance as a criterion for RTS and physical activities in patients after ACL reconstruction. Higher quality studies are necessary to establish the extent of association between these variables.
Effects of Training Frequency During a 6-Month Neuromuscular Training Intervention on Movement Competency, Strength, and Power in Male Youth
Authors: Lloyd Rhodri S. , Dobbs Ian J. , Wong Megan A. , Moore Isabel S. , Oliver Jon L. 
Published: October 2021

Background: Youth populations benefit from neuromuscular training; however, the extent to which training frequency, baseline fitness, and maturity status moderate the response to long-term neuromuscular training in male youth remains unclear. Hypothesis: Twice-weekly training (G2x) would induce larger improvements in movement competency and strength and power kinetics compared with once-weekly training (G1x). Maturity status and baseline fitness would also moderate the training response in strength and power kinetics. Study Design: Nonrandomized, repeated-measures design. Level of Evidence: Level 3 Methods: Ninety-five male athletes of varying maturity status (pre– or post–peak height velocity) were divided into G2x, G1x, or control (CON) groups. Training groups received G2x or G1x for 28 weeks. Back squat assessment (BSA) movement competency and isometric mid-thigh pull (IMTP), squat jump (SJ), and countermovement jump (CMJ) kinetics were measured pre- and postintervention. Results: The G2x achieved significantly greater adaptations in BSA total score, IMTP allometrically scaled peak force (PFallo), IMTP peak rate-of-force development (PRFD), SJ PFallo and PRFD compared with G1x and CON ( P < 0.05; d = 0.3-0.9). Baseline fitness, training frequency and maturity status were significant predictors of IMTP PFallo ( R2 = 27.5%) and PRFD ( R2 = 42.9%), while a combination of training frequency and baseline fitness explained a small amount of variance in SJ PFallo ( R2 = 12.2%) and SJ PRFD ( R2 = 6.5%). There were no significant predictors of CMJ kinetics. Conclusion: G2x achieved significantly larger improvements in movement competency, isometric strength and concentric jump variables compared with G1x, with the training response greater in those youth with lower baseline fitness and more advanced maturity status. Clinical Relevance: Exposure to neuromuscular training twice-weekly elicits a greater response than G1x in young male athletes. Practitioners should take maturity status, training frequency, and baseline fitness levels of young male athletes into account when interpreting testing data, as these variables appear to moderate training responsiveness.
Effect of Exercise Training on 1,25(OH)2D Levels: The FIT-AGEING Randomized Controlled Trial
Authors: De-la-O Alejandro , Jurado-Fasoli Lucas , Castillo Manuel J. , Gutiérrez Ángel , Amaro-Gahete Francisco J. 
Published: October 2021

Background: Vitamin D deficiency is currently endemic worldwide and is considered as an important factor in the development of several chronic conditions. Physical exercise has been postulated as an auspicious strategy to counteract age-related disorders preventing premature mortality. However, the effects of chronic exercise training on 1,25-dihydroxyvitamin D [1,25(OH)2D] is unclear. This 12-week randomized controlled trial aimed to investigate the effects of different training modalities on 1,25(OH)2D in healthy sedentary adults. Hypothesis: Exercise training will increase 1,25(OH)2D in the study cohort. Study Design: Randomized controlled clinical trial. Level of Evidence: Level 1. Methods: A total of 89 healthy sedentary adults (53% women; 53.5 ± 4.9 years old) were enrolled in the FIT-AGEING study. The participants were randomized to (1) a control group (no exercise), (2) physical activity recommendation from the World Health Organization (PAR group), (3) high-intensity interval training (HIIT group), and (4) HIIT adding whole-body electromyostimulation training (HIIT + EMS). 1,25(OH)2D plasma levels were measured using a DiaSorin Liaison immunochemiluminometric analyzer. Results: Compared with the control group, 1,25(OH)2D increased in PAR (Δ = 10.99 ± 3.44 pg/mL; P = 0.01), HIIT (Δ = 11.63 ± 3.51 pg/mL; P = 0.009), and HIIT + EMS groups (Δ = 14.01 ± 3.59 pg/mL; P = 0.001) without statistical differences between them (all Ps > 0.1). Conclusion: In summary, the results show that a 12-week exercise intervention produced an increment of 1,25(OH)2D independently of age, sex, and exercise modality in healthy sedentary adults. Clinical Relevance: The implementation of physical exercise could be considered a strategy not only aiming to reverse the seasonal decrease of 1,25(OH)2D in winter explained by low sunlight exposure but also for obtaining subsequent increases of this hormone even in these a priori adverse conditions.
Investigating the Role of Low-FODMAP Diet in Improving Gastrointestinal Symptoms in Irritable Bowel Syndrome
Authors: Yang Jiahong , Lin Gina Ziruo , Li Xiao Ying , Wang Yu Tien , Loh Yet Hua 
Published: October 2021

Background A low–fermentable oligosaccharide, disaccharide, monosaccharide, and polyol (FODMAP) diet (LFD) appeared to improve gastrointestinal symptoms in patients with Irritable Bowel Syndrome (IBS). However, evidence of its efficacy in Asia remains scarce. Objectives This study aims to investigate the clinical effectiveness of LFD intervention among Asian IBS patients and barriers to its adherence. Methods Patients with IBS who were instructed to follow LFD by dietitians at a tertiary hospital in Singapore between July 2013 and December 2014 were retrospectively reviewed. Severity scores of 19 gastrointestinal symptoms were recorded using an 11-point rating scale (0 = “no symptoms,” 10 = “extremely severe”) pre- and post-LFD intervention. Adherence to LFD was assessed based on patients’ diet history and a high-FODMAP food checklist. Results Eighty-two patients with a median follow-up of 5.8 weeks were investigated. The LFD adherence rate was 61.0% ( n = 50). Overall, statistically significant improvement was observed in 14 of 19 gastrointestinal symptoms (median difference = −2.0 to −0.5, p < .05). The proportion of patients who had improvement in 10 individual symptoms (defined as ≥1 point reduction in symptom severity rating) was significantly higher in LFD-adherent patients (67.5%–82.4%) than that of the non-adherent group (27.8%–55.0%). Reasons reported for non-adherence included “limited availability of low-FODMAP alternatives” ( n = 9), “limited understanding of LFD” ( n = 7), and “perceived lack of benefit” ( n = 4). Conclusions LFD intervention improved gastrointestinal symptoms in a cohort of Asian IBS patients. Limited access to low-FODMAP alternatives was the main barrier to adherence.
Circulating microRNA-194 levels in Chinese patients with diabetic kidney disease: a case–control study
Authors: Ma Ning , Xu Ning , Yin Dong , Zheng Ping , Liu Weiwei , Wang Guofeng , Hui Yuan , Zhang Jiping , Han Guanjun , Yang Chuanhui , Chen Yiting , Cheng Xingbo , Cheng Ming 
Published: October 2021

Objective: MicroRNAs (miRNAs) regulate gene expression and are involved in diabetic kidney disease (DKD) pathogenesis. We investigated circulating miRNA-194 levels as a biomarker of DKD prevalence and incidence, and the relationship between miRNA-194 and CCAAT/enhancer binding protein (C/EBP) homologous protein (CHOP). Methods: We recruited 136 type-2 diabetes mellitus (T2DM) patients at the First People’s Hospital of Lianyungang and 127 healthy individuals. Circulating miRNA-194 and CHOP levels were measured using quantitative reverse transcription qRT-PCR and enzyme-linked immunosorbent assay (ELISA), respectively. Anthropometric and biochemistry measurements were also made. Results: T2DM patients showed higher circulating miRNA-194 ( p = 0.029) and lower circulating CHOP ( p < 0.001) levels than controls. Circulating miRNA-194 levels were significantly higher in T2DM patients with a microalbumin/creatinine ratio (UmALB/Cr) ⩾ 300 mg/g ( p < 0.001). In addition, there were significant intergroup differences in the circulating CHOP concentrations ( p = 0.005). Bivariate analysis revealed that circulating miR-194 levels were negatively correlated with alpha-fetoprotein and CHOP levels ( r = −0.222, −0.301; p = 0.018, 0.001, respectively), but positively correlated with fasting glucose, UmALB/Cr, Cr, Cystatin C, quantitative insulin check index (QUICKI) ( r = 0.193, 0.446, 0.260, 0.339, and 0.250, respectively; p = 0.036, <0.001, 0.005, <0.001, and 0.006, respectively), particularly UmALB/Cr and Cystatin C ( p < 0.001). Logistic regression analysis after adjusting for covariates associated with UmALB/Cr identified duration of T2DM, systolic blood pressure, Cr, estimated glomerular filtration rate, and waist circumference as independent factors associated with T2DM patients with UmALB/Cr > 300 ( p = 0.030, 0.013, <0.001, <0.001, and 0.031, respectively). Conclusion: Circulating miRNA-194 levels could be a novel biomarker for DKD.
Targeting Rule Implementation Decreases Concussions in High School Football: A National Concussion Surveillance Study
Authors: Obana Kyle K. , Mueller John D. , Saltzman Bryan M. , Bottiglieri Thomas S. , Ahmad Christopher S. , Parisien Robert L. , Trofa David P. 
Published: October 2021

Background: Concussions occur at higher rates in high school football as compared with all other high school sports. In 2014, the National Federation of State High School Associations implemented rules defining illegal contact against a defenseless player above the shoulders to reduce concussions in football players in the United States. To the best of our knowledge, rates of emergency department (ED)—diagnosed concussions of high school football players before and after the 2014 rule implementation have not been compared. Hypothesis: It was hypothesized that (1) there would be lower rates of helmet-to-helmet and helmet-to-body-part concussions after rule implementation and (2) alternative mechanisms of concussion would not differ, as these would be less influenced by rule implementation. Study Design: Cohort study; Level of evidence, 3. Methods: Data from the National Electronic Injury Surveillance System (NEISS) were analyzed for high school football players 14 to 18 years old sustaining a concussion from January 1, 2009, to December 31, 2019. Data were collected on mechanism of injury, setting, and loss of consciousness. Raw data were used to calculate national estimates based on the assigned statistical sample weight of each hospital by the NEISS. Results: A total of 4983 (national estimate = 154,221) high school football concussions were diagnosed in US EDs; 58.8% of concussions occurred during competition and 41.2% during practice. Between 2009 and 2013 the rate of concussions diagnosed in EDs rose 10.7% as compared with a 6.2% decrease between 2015 and 2019 ( P = .04). Between 2009 and 2013, the rate of helmet-to-helmet concussions rose 17.6% as compared with a 5.6% decrease between 2015 and 2019 ( P = .03). There were no significant changes between other mechanisms of concussion before and after the 2014 rule implementation. Conclusion: We identified a decreased trend in overall and helmet-to-helmet high school football concussions diagnosed in the ED after implementation of the targeting rule. This study adds to the growing literature regarding the importance and efficacy of rule implementation in reducing sports-related concussions.
d-Serine produces antidepressant-like effects in mice through suppression of BDNF signaling pathway and regulation of synaptic adaptations in the nucleus accumbens
Authors: Chen Zhenzhen , Tang Zhenyu , Zou Ke , Huang Zhihong , Liu Liuer , Yang Yuanjian , Wang Wei 
Published: October 2021

AbstractObjectived-Serine is a crucial endogenous co-agonist of N-methyl-d-aspartate receptors (NMDARs) in the central nervous system and can affect the function of the brain derived neurotrophic factor (BDNF) system, which plays an essential role in modulating synaptic plasticity. The current study aimed to systematically evaluate the role and mechanisms ofd-serine in depressive behavior in nucleus accumbens (NAc).Methodsd-Serine concentration in the chronic social defeat stress (CSDS) model in NAc was measured using high-performance liquid chromatography (HPLC). The antidepressant-like effects ofd-serine were identified using forced swim test (FST) and tail suspension test (TST) in control mice and then assessed in CSDS model. We applied social interaction and sucrose preference tests to identify the susceptibility of CSDS model. Western blotting was further performed to assess the changes of BDNF signaling cascade in NAc after CSDS andd-serine treatment. The BDNF signaling inhibitor (K252a) was also used to clarify the antidepressant-like mechanism ofd-serine. Moreover,d-serine effects on synaptic plasticity in NAc were investigated using electrophysiological methods.Resultsd-Serine concentration was decreased in depression susceptible mice in NAc.d-Serine injections into NAc exhibited antidepressant-like effects in FST and TST without affecting the locomotor activity of mice.d-Serine was also effective in CSDS model of depression. Moreover,d-serine down-regulated the BDNF signaling pathway in NAc during CSDS procedure. Furthermore, BDNF signaling inhibitor (K252a) enhanced the antidepressant effects ofd-serine. We also found thatd-serine was essential for NMDARs-dependent long-term depression (LTD).Conclusiond-Serine exerts antidepressant-like effects in mice mediated through restraining the BDNF signaling pathway and regulating synaptic plasticity in NAc.
The one-step pyrolysis process of rattan-based silicon carbide multiphase ceramics prepared by sol–gel method
Authors: Li Shijie , Cui Heshuai , Ma Qianli , Liu Xing’e , Wang Youhong , Shang Lili , Zheng Yu 
Published: October 2021

AbstractThe sol–gel method was used to prepare rattan-based silicon carbide (R–SiC) composite ceramics under different pyrolysis parameters through adjustment of the temperature and retention time of the one-step pyrolysis process. The crystalline phases, microscopic morphology, element distribution and specific surface area of the silicon carbide (SiC) were characterized by X-ray diffraction (XRD), Fourier transform-infrared spectroscopy (FT-IR), X-ray fluorescence spectrometer (XRF), field-emission scanning electron microscope (FESEM), transmission electron microscopy (TEM), energy dispersive X-ray analysis (EDX), and N2 physisorption. The results showed that the R–SiC prepared at different pyrolysis parameters was able to retain the porous structure of pristine rattan stem. The R–SiC prepared at 1500 ℃ for 120 min possessed the lowest density (0.25 g/cm3), the largest specific surface area (43.38 m2/g) and the highest SiC yield (44.24%). The SiC whisker was the major SiC morphology on the cross section of the R–SiC. Furthermore, the pyrolysis parameters were optimized with the SiC preparation process reaction mechanism, and material transformation methods were also discussed. This one-step pyrolysis process simplified the preparation of biogenic SiC ceramics and thus provided a potential route for the value-added utilization of rattan.
Annotation depth confounds direct comparison of gene expression across species
Authors: Oziolor Elias , Arat Seda , Martin Matthew 
Published: October 2021

Abstract
Background
Comparisons of the molecular framework among organisms can be done on both structural and functional levels. One of the most common top-down approaches for functional comparisons is RNA sequencing. This estimation of organismal transcriptional responses is of interest for understanding evolution of molecular activity, which is used for answering a diversity of questions ranging from basic biology to pre-clinical species selection and translation. However, direct comparison between species is often hindered by evolutionary divergence in structure of molecular framework, as well as large difference in the depth of our understanding of the genetic background between humans and other species. Here, we focus on the latter. We attempt to understand how differences in transcriptome annotation affect direct gene abundance comparisons between species.

Results
We examine and suggest some straightforward approaches for direct comparison given the current available tools and using a sample dataset from human, cynomolgus monkey, dog, rat and mouse with a common quantitation and normalization approach. In addition, we examine how variation in genome annotation depth and quality across species may affect these direct comparisons.

Conclusions
Our findings suggest that further efforts for better genome annotation or computational normalization tools may be of strong interest.
Transcriptome and de novo analysis of Rosa xanthina f. spontanea in response to cold stress
Authors: Zhuang Defeng , Ma Ce , Xue Li , Li Zhen , Wang Cheng , Lei Jiajun , Yuan Xingfu 
Published: October 2021

Abstract
Background
Rose is one of most popular ornamental plants worldwide and is of high economic value and great cultural importance. However, cold damage restricts its planting application in cold areas. To elucidate the metabolic response of rose under low temperature stress, we conducted transcriptome and de novo analysis of Rosa xanthina f. spontanea.

Results
A total of 124,106 unigenes from 9 libraries were generated by de novo assembly, with N50 length was 1470 bp, under 4 °C and − 20 °C stress (23 °C was used as a control). Functional annotation and prediction analyses identified 55,084 unigenes, and 67.72% of these unigenes had significant similarity (BLAST, E ≤ 10− 5) to those in the public databases. A total of 3031 genes were upregulated and 3891 were downregulated at 4 °C compared with 23 °C, and 867 genes were upregulated and 1763 were downregulated at − 20 °C compared with 23 °C. A total of 468 common DEGs were detected under cold stress, and the matched DEGs were involved in three functional categories: biological process (58.45%), cellular component (11.27%) and molecular function (30.28%). Based on KEGG functional annotations, four pathways were significantly enriched: metabolic pathway, response to plant pathogen interaction (32 genes); starch and sucrose metabolism (21 genes); circadian rhythm plant (8 genes); and photosynthesis antenna proteins (7 genes).

Conclusions
Our study is the first to report the response to cold stress at the transcriptome level in R. xanthina f. spontanea. The results can help to elucidate the molecular mechanism of cold resistance in rose and provide new insights and candidate genes for genetically enhancing cold stress tolerance.
Genome-wide survey of Gγ subunit gene family in eight Rosaceae and expression analysis of PbrGGs in pear (Pyrus bretschneideri)
Authors: Chen Guodong , Li Yang , Qiao Xin , Duan Weike , Jin Cong , Cheng Rui , Wang Jizhong 
Published: October 2021

Abstract
Background
Heterotrimeric G-proteins, composed of Gα, Gβ and Gγ subunits, are important signal transmitters, mediating the cellular response to multiple stimuli in animals and plants. The Gγ subunit is an essential component of the G-protein, providing appropriate functional specificity to the heterotrimer complex and has been well studied in many species. However, the evolutionary history, expression pattern and functional characteristics of Gγ subunits has not been explored in the Rosaceae, representing many important fruit crops.

Results
In this study, 35 Gγ subunit genes were identified from the eight species belonging to the Rosaceae family. Based on the structural gene characteristics, conserved protein motifs and phylogenetic analysis of the Gγ subunit genes, the genes were classified into three clades. Purifying selection was shown to play an important role in the evolution of Gγ subunit genes, while a recent whole-genome duplication event was the principal force determining the expansion of the Gγ subunit gene family in the subfamily Maloideae. Gγ subunit genes exhibited diverse spatiotemporal expression patterns in Chinese white pear, including fruit, root, ovary and bud, and under abiotic stress conditions, the relative expression of Gγ subunit genes were up-regulated or down-regulated. In addition, seven of the Gγ subunit proteins in pear were located on the plasma membrane, in the cytoplasm or nucleus.

Conclusion
Overall, this study of the Gγ subunit gene family in eight Rosaceae species provided useful information to better understand the evolution and expression of these genes and facilitated further exploration of their functions in these important crop plants.