bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effects of individual or in‐combination antioxidant supplementation during in vitro maturation culture on the developmental competence and quality of porcine embryos
Authors: Thongkittidilok Chommanart , Le Quynh Anh , Lin Qingyi , Takebayashi Koki , Do Thi Kim Lanh , Namula Zhao , Hirata Maki , Tanihara Fuminori , Otoi Takeshige 
Published: December 2021

AbstractThe oocyte maturation process requires a high supply of energy, which generates reactive oxygen species (ROS), adversely affecting oocyte and embryo development. Balancing ROS by antioxidant supplementation is essential for maintaining oocyte maturation and embryonic quality in vitro. This study aimed to evaluate the impact of four antioxidants: β‐mercaptoethanol (β‐ME), chlorogenic acid (CGA), curcumin and sericin, when applied individually or in combinations, during oocyte maturation on development of porcine oocytes. Cumulus–oocyte complexes were collected, cultured in maturation medium supplemented with antioxidants for 44 hr and subsequently subjected to in vitro fertilization (IVF) and culture for 7 days. Combining all four (β‐ME + CGA + curcumin + sericin) or three (β‐ME + CGA + curcumin) antioxidants increased blastocyst formation rates. However, sericin supplementation alone, or in combination with β‐ME or CGA, failed to improve blastocyst formation rates. The total cell numbers of blastocysts from the group supplemented with three antioxidants (β‐ME + CGA + curcumin) were significantly higher than those from the other groups, except for the curcumin‐supplement group. There were no differences in the maturation rates and proportions of oocytes with fragmented DNA between the antioxidant‐supplemented and the non‐supplemented control groups. In conclusion, supplementation with three antioxidants (β‐ME + CGA + curcumin) during the maturation culture enhanced blastocyst formation and improved blastocyst quality.
Profiles of psychosocial factors: Can they be used to predict injury risk?
Authors: Clement Damien , Tranaeus Ulrika , Johnson Urban , Stenling Andreas , Ivarsson Andreas 
Published: December 2021

The creation of risk profiles using the model of stress and athletic injury (J Appl Sport Psychol. 1998;10(1):5) represents a proposed shift from the reductionism paradigm to the complex sport approach in an attempt to formulate prevention strategies to combat the increasing number of injuries being reported in sporting populations. As a result, the primary purpose of this study was to: (a) identify different risk profiles based on psychosocial factors associated with the Williams and Andersen's model of stress and athletic injury model; and (b) examine potential differences in the frequency of injuries across these risk profiles. A prospective research design was utilized with a sample of 117 competitive soccer players (81 males and 36 females) from Sweden and the United States of America. Data was collected at two time points over the course of three months. At time 1 (beginning of the season) ‐ a demographic information sheet, the Life Event Survey for Collegiate Athletes (LESCA), Sport Competitive Anxiety Test (SCAT), and Brief Cope were administered. At time two (T2), three months after the initial data collection, participants’ traumatic injuries were recorded. Latent profile analysis (LPA) showed that 3 profiles solution showed best fit to data. Players in profile 1 and 2 reported fewer injuries compared to players in profile 3. However, whereas individuals in profile 1 had a lower predictive risk of sustaining an injury when compared to those in profile 3, both profiles had similar anxiety levels and use of coping strategies with differing stress levels. These findings suggest that the interaction between different proposed risk factors might influence injury risk.
The changing tides of attractive alternatives in romantic relationships: Recent societal changes compel new directions for future research
Authors: Brady Ashlyn , Baker Levi R. 
Published: December 2021

AbstractSocietal changes over recent decades have drastically transformed the frequency and manner in which people are exposed to attractive alternative relationship partners, arguably resulting in such alternatives posing a greater threat to committed relationships now than ever before. Yet despite a growing need for novel research on attractive alternatives, research on this topic has failed to account for such changes and thus is growing stagnant. Specifically, although interdependence perspectives and supporting research have consistently and robustly demonstrated that (a) attractive alternatives threaten committed relationships and (b) committed partners protect their relationships by devaluing such alternatives, research has yet to examine how the changing nature of attractive alternatives might affect these processes. To this end, the present article first reviews foundational theory and research that guided the study of attractive alternatives and then highlights how recent societal changes (e.g., technology that increases access to attractive alternatives, increasingly diverse relationship types, the emerging desire to remain single) diverge from this research and thus warrant new directions. We encourage researchers to expand how they study attractive alternatives and to ultimately reignite research on this increasingly important topic.
Jujube metabolome selection determined the edible properties acquired during domestication
Authors: Zhang Zhong , Shi Qianqian , Wang Bin , Ma Aimin , Wang Yongkang , Xue Qingtun , Shen Bingqi , Hamaila Halina , Tang Tang , Qi Xiaoquan , Fernie Alisdair R. , Luo Jie , Li Xingang 
Published: December 2021

SUMMARYPlants supply both food and medicinal compounds, which are ascribed to diverse metabolites produced by plants. However, studies on domestication‐driven changes in the metabolome and genetic basis of bioactive molecules in perennial fruit trees are generally lacking. Here, we conducted multidimensional analyses revealing a singular domestication event involving the genomic and metabolomic selection of jujube trees (Ziziphus jujuba Mill.). The genomic selection for domesticated genes was highly enriched in metabolic pathways, including carbohydrates and specialized metabolism. Domesticated metabolome profiling indicated that 187 metabolites exhibited significant divergence as a result of directional selection. Malic acid was directly selected during domestication, and the simultaneous selection of specialized metabolites, including triterpenes, consequently lead to edible properties. Cyclopeptide alkaloids (CPAs) were specifically targeted for the divergence between dry and fresh cultivars. We identified 1080 significantly associated loci for 986 metabolites. Among them, 15 triterpenes were directly selected at six major loci, allowing the identification of a homologous cluster containing seven 2,3‐oxidosqualene cyclases (OSCs). An OSC gene was found to contribute to the reduction in the content of triterpenes during domestication. The complete pathway for synthesizing ursolic acid was dissected by integration of the metabolome and transcriptome. Additionally, an N‐methyltransferase involved in the biosynthesis of CPA and responsible for inter‐cultivar content variation was identified. The present study promotes our understanding of the selection process of the global metabolome subsequent to fruit tree domestication and facilitates the genetic manipulation of specialized metabolites to enhance their edible traits.
ODORANT1 targets multiple metabolic networks in petunia flowers
Authors: Boersma Maaike R. , Patrick Ryan M. , Jillings Sonia L. , Shaipulah Nur Fariza M. , Sun Pulu , Haring Michel A. , Dudareva Natalia , Li Ying , Schuurink Robert C. 
Published: December 2021

SUMMARYScent bouquets produced by the flowers of Petunia spp. (petunia) are composed of a complex mixture of floral volatile benzenoid and phenylpropanoid compounds (FVBPs), which are specialized metabolites derived from phenylalanine (Phe) through an interconnected network of enzymes. The biosynthesis and emission of high levels of these volatiles requires coordinated transcriptional activation of both primary and specialized metabolic networks. The petunia R2R3‐MYB transcription factor ODORANT 1 (ODO1) was identified as a master regulator of FVBP production and emission; however, our knowledge of the direct regulatory targets of ODO1 has remained limited. Using chromatin immunoprecipitation followed by sequencing (ChIP‐seq) in petunia flowers, we identify genome‐wide ODO1‐bound genes that are enriched not only in genes involved in the biosynthesis of the Phe precursor, as previously reported, but also genes associated with the specialized metabolic pathways involved in generating phenylpropanoid intermediates for FVBPs. ODO1‐bound genes are also involved in methionine and S‐adenosylmethionine metabolism, which could modulate methyl group supplies for certain FVBPs. Quantitative reverse transcription polymerase chain reaction (qRT‐PCR) and RNA‐seq analysis in an ODO1 RNAi knockdown line revealed that ODO1‐bound targets are expressed at lower levels when ODO1 is suppressed. A cis‐regulatory motif, CACCAACCCC, was identified as a potential binding site for ODO1 in the promoters of genes that are both bound and activated by ODO1, which was validated by in planta promoter reporter assays with wild‐type and mutated promoters. Overall, our work presents a mechanistic model for ODO1 controlling an extensive gene regulatory network that contributes to FVBP production to give rise to floral scent.
Inter‐tissue and inter‐organ signaling in drought stress response and phenotyping of drought tolerance
Authors: Kuromori Takashi , Fujita Miki , Takahashi Fuminori , Yamaguchi‐Shinozaki Kazuko , Shinozaki Kazuo 
Published: December 2021

SUMMARYPlant response to drought stress includes systems for intracellular regulation of gene expression and signaling, as well as inter‐tissue and inter‐organ signaling, which helps entire plants acquire stress resistance. Plants sense water‐deficit conditions both via the stomata of leaves and roots, and transfer water‐deficit signals from roots to shoots via inter‐organ signaling. Abscisic acid is an important phytohormone involved in the drought stress response and adaptation, and is synthesized mainly in vascular tissues and guard cells of leaves. In leaves, stress‐induced abscisic acid is distributed to various tissues by transporters, which activates stomatal closure and expression of stress‐related genes to acquire drought stress resistance. Moreover, the stepwise stress response at the whole‐plant level is important for proper understanding of the physiological response to drought conditions. Drought stress is sensed by multiple types of sensors as molecular patterns of abiotic stress signals, which are transmitted via separate parallel signaling networks to induce downstream responses, including stomatal closure and synthesis of stress‐related proteins and metabolites. Peptide molecules play important roles in the inter‐organ signaling of dehydration from roots to shoots, as well as signaling of osmotic changes and reactive oxygen species/Ca2+. In this review, we have summarized recent advances in research on complex plant drought stress responses, focusing on inter‐tissue signaling in leaves and inter‐organ signaling from roots to shoots. We have discussed the mechanisms via which drought stress adaptations and resistance are acquired at the whole‐plant level, and have proposed the importance of quantitative phenotyping for measuring plant growth under drought conditions.
Hydroxycinnamic acid‐modified xylan side chains and their cross‐linking products in rice cell walls are reduced in the Xylosyl arabinosyl substitution of xylan 1 mutant
Authors: Feijao Carolina , Morreel Kris , Anders Nadine , Tryfona Theodora , Busse‐Wicher Marta , Kotake Toshihisa , Boerjan Wout , Dupree Paul 
Published: December 2021

SUMMARYThe intricate architecture of cell walls and the complex cross‐linking of their components hinders some industrial and agricultural applications of plant biomass. Xylan is a key structural element of grass cell walls, closely interacting with other cell wall components such as cellulose and lignin. The main branching points of grass xylan, 3‐linked l‐arabinosyl substitutions, can be modified by ferulic acid (a hydroxycinnamic acid), which cross‐links xylan to other xylan chains and lignin. XAX1 (Xylosyl arabinosyl substitution of xylan 1), a rice (Oryza sativa) member of the glycosyltransferase family GT61, has been described to add xylosyl residues to arabinosyl substitutions modified by ferulic acid. In this study, we characterize hydroxycinnamic acid‐decorated arabinosyl substitutions present on rice xylan and their cross‐linking, in order to decipher the role of XAX1 in xylan synthesis. Our results show a general reduction of hydroxycinnamic acid‐modified 3‐linked arabinosyl substitutions in xax1 mutant rice regardless of their modification with a xylosyl residue. Moreover, structures resembling the direct cross‐link between xylan and lignin (ferulated arabinosyl substitutions bound to lignin monomers and dimers), together with diferulates known to cross‐link xylan, are strongly reduced in xax1. Interestingly, apart from feruloyl and p‐coumaroyl modifications on arabinose, putative caffeoyl and oxalyl modifications were characterized, which were also reduced in xax1. Our results suggest an alternative function of XAX1 in the transfer of hydroxycinnamic acid‐modified arabinosyl substitutions to xylan, rather than xylosyl transfer to arabinosyl substitutions. Ultimately, XAX1 plays a fundamental role in cross‐linking, providing a potential target for the improvement of use of grass biomass.
Prolyl‐4‐hydroxylases 2 and 3 control erythropoietin production in renin‐expressing cells of mouse kidneys
Authors: Broeker Katharina A. E. , Fuchs Michaela A. A. , Schrankl Julia , Lehrmann Claudia , Schley Gunnar , Todorov Vladimir T. , Hugo Christian , Wagner Charlotte , Kurtz Armin 
Published: December 2021

AbstractActivation of the hypoxia‐signalling pathway induced by deletion of the ubiquitin‐ligase von Hippel–Lindau protein causes an endocrine shift of renin‐producing cells to erythropoietin (EPO)‐expressing cells. However, the underlying mechanisms have not yet been investigated. Since oxygen‐regulated stability of hypoxia‐inducible transcription factors relevant for EPO expression is dependent on the activity of prolyl‐4‐hydroxylases (PHD) 2 and 3, this study aimed to determine the relevance of different PHD isoforms for the EPO expression in renin‐producing cells in vivo. For this purpose, mice with inducible renin cell‐specific deletions of different PHD isoforms were analysed. Our study shows that there are two subgroups of renal renin‐expressing cells, juxtaglomerular renin+ cells and platelet‐derived growth factor receptor‐β+ interstitial renin+ cells. These interstitial renin+ cells belong to the cell pool of native EPO‐producing cells and are able to express EPO and renin in parallel. In contrast, co‐deletion of PHD2 and PHD3, but not PHD2 deletion alone, induces EPO expression in juxtaglomerular and hyperplastic renin+ cells and downregulates renin expression. A strong basal PHD3 expression in juxtaglomerular renin+ cells seems to prevent the hypoxia‐inducible transcription factor‐2‐dependent phenotype shift into EPO cells. In summary, PHDs seem important for the stabilization of the juxtaglomerular renin cell phenotype. Moreover, these findings reveal tubulointerstitial cells as a novel site of renal renin expression and suggest a high endocrine plasticity of these cells. Our data concerning the distinct expression patterns and functions of PHD2 and PHD3 provide new insights into the regulation of renin‐producing cells and highlight the need for selective PHD inhibitors.Key points
Renal renin‐expressing cells can be clearly distinguished into two subgroups, the typical juxtaglomerular renin‐producing cells and interstitial renin+ cells.
Interstitial renin+ cells belong to the cell pool of native erythropoietin (EPO)‐producing cells, show a fast EPO response to acute hypoxia‐inducible factor‐2 (HIF‐2) stabilization and are able to express EPO and renin in parallel.
Only co‐deletion of the prolyl‐4‐hydroxylases (PHD) 2 and 3, but not PHD2 deletion alone, induces EPO expression in juxtaglomerular renin+ cells.
Chronic HIF‐2 stabilization in juxtaglomerular renin‐expressing cells leads to their phenotypic shift into EPO‐producing cells.
A strong basal PHD3 expression in juxtaglomerular renin+ cells seems to prevent a HIF‐2‐dependent phenotype shift into EPO cells suggesting PHD3 fulfils a stabilizer function for the juxtaglomerular renin cell phenotype.
Ordovician and Mississippian stratigraphy in southwestern Missouri, USA
Authors: Rovey Charles W. , Bassett Damon J. , McKay Matthew P. 
Published: December 2021

ABSTRACT
A succession of Ordovician and Mississippian carbonates, separated unconformably, is exposed across the southern flank of the Ozark Dome in southwest Missouri. Deposits of both periods exemplify typical facies of the Midwestern United States: carbonate tidal-flat assemblages for the Early Ordovician and carbonate shelf environments for the Early–Middle Mississippian. The basic stratigraphic sequence of these deposits has been known for over a century, but interesting features remain to be addressed. Thin discontinuous sandstones are present within the Early Ordovician Cotter Dolomite, but the informal Swan Creek sandstone member seems anomalous. This sandstone can exceed 5 m in thickness and is fairly continuous across southwest Missouri. Most Ordovician sandstones in Missouri mark major transgressions above regional unconformities, but not the Swan Creek, and there is no obvious source of the sand. Therefore, we hypothesize that the Swan Creek represents reworked eolian dunes blown across the broad peritidal environment. Clastic sandstone dikes, apparently sourced from the Swan Creek, cut across beds of Cotter Dolomite near faults. We propose that these dikes are evidence of local faulting and seismicity during the Early Ordovician.
Early and Middle Mississippian limestones comprise a sequence of shelf deposits, although mud mounds and other facies changes near the Missouri-Arkansas line mark the edge of the Mississippian shelf and the transition to a ramp setting. Early Mississippian carbonate deposition was interrupted by a short and localized influx of siliciclastic sediment comprising the Northview Formation. The Northview has additional characteristics consistent with a river-dominated deltaic deposit, which we suggest as its origin. If correct, this hypothesis implies that the history of tectonic features in the Midwest is more complicated than yet known. Finally, facies changes within and between the local Mississippian formations may record an early crustal response to the impending Ouachita orogeny farther to the south.
Electrical Properties of Li+-Substituted Solid Solutions Based on Gd2Zr2O7
Authors: Anokhina I. A. , Animitsa I. E. , Voronin V. I. , Vykhodets V. B. , Kurennykh T. E. , Buzina A. F. , Kazakova V. N. , Nokhrin S. S. , Zaikov Yu. P. 
Published: December 2021

Abstract
Solid solution $${\text{G}}{{{\text{d}}}_{{2 - x}}}{\text{L}}{{{\text{i}}}_{x}}{\text{Z}}{{{\text{r}}}_{2}}{{{\text{O}}}_{{7 - x}}}$$ with a pyrochlore structure is synthesized for the first time. The cationic composition is confirmed via chemical analysis and nuclear reactions. It is found that the stoichiometry with respect to lithium is retained up to 1100°C. The lattice parameter diminishes in the homogeneity range 0 ≤ x ≤ 0.30, while the free volume of migration grows. Introducing lithium into the Gd sublattice raises oxygen–ion conductivity, due to the emergence of oxygen vacancies and enhancement of their mobility. Maximum conductivity is reached for composition with х = 0.10 (~1 × 10−3 Ω−1 cm−1, 650°C). An assumption is made about the formation of associates of the type $${{\{ {\text{Li}}_{{{\text{Gd}}}}^{{''}} \cdot {\text{V}}_{{\text{o}}}^{{ \bullet \bullet }}\} }^{ \times }}$$ at high contents of the dopant (x = 0.30), accompanied by an increase in the activation energy of conductivity.
Thermodynamic Modeling of Multicomponent Rare Earth Nitrates Aqueous Systems
Authors: Dzuban A. V. , Galstyan A. A. , Kovalenko N. A. , Uspenskaya I. A. 
Published: December 2021

Abstract
Solubility constants of rare earth (RE) nitrates crystalline hydrates are determined in a wide temperature range (−30 to 120°C), salts solubilities and phase diagrams of water–RE nitrate systems are calculated. For multicomponent (n > 5) solutions of RE nitrates the assessment of solution properties as well as phase diagrams are shown to be feasible within experimental uncertainty. In case of mixtures of RE nitrates with similar hydrodynamic radii of ions, the parameters of RE1–RE2 interparticle interaction can be ignored without losing accuracy of thermodynamic modeling.
Improved $${(g-2)_\mu }$$ measurements and wino/higgsino dark matter
Authors: Chakraborti Manimala , Heinemeyer Sven , Saha Ipsita 
Published: December 2021

AbstractThe electroweak (EW) sector of the Minimal Supersymmetric Standard Model (MSSM) can account for a variety of experimental data. In particular it can explain the persistent $$3-4\,\sigma $$

3
-
4

σ

discrepancy between the experimental result for the anomalous magnetic moment of the muon, $$(g-2)_\mu $$

(
g
-
2
)

μ

, and its Standard Model (SM) prediction. The lightest supersymmetric particle (LSP), which we take as the lightest neutralino, $${\tilde{\chi }}_{1}^0$$

χ
~

1

0

, can furthermore account for the observed Dark Matter (DM) content of the universe via coannihilation with the next-to-LSP (NLSP), while being in agreement with negative results from Direct Detection (DD) experiments. Concerning the unsuccessful searches for EW particles at the LHC, owing to relatively small production cross-sections a comparably light EW sector of the MSSM is in full agreement with the experimental data. The DM relic density can fully be explained by a mixed bino/wino LSP. Here we take the relic density as an upper bound, which opens up the possibility of wino and higgsino DM. We first analyze which mass ranges of neutralinos, charginos and scalar leptons are in agreement with all experimental data, including relevant LHC searches. We find roughly an upper limit of $$\sim 600 \,\, \mathrm {GeV}$$

∼
600

GeV

for the LSP and NLSP masses. In a second step we assume that the new result of the Run 1 of the “MUON G-2” collaboration at Fermilab yields a precision comparable to the existing experimental result with the same central value. We analyze the potential impact of the combination of the Run 1 data with the existing $$(g-2)_\mu $$

(
g
-
2
)

μ

data on the allowed MSSM parameter space. We find that in this case the upper limits on the LSP and NLSP masses are substantially reduced by roughly $$100 \,\, \mathrm {GeV}$$

100

GeV

. We interpret these upper bounds in view of future HL-LHC EW searches as well as future high-energy $$e^+e^-$$

e
+

e
-

colliders, such as the ILC or CLIC.
Persian in the Villages, or, the Language of Jamiat Rai’s Account Books
Authors: Sheikh Samira 
Published: December 2021

Abstract
District (pargana)-level land revenue administration in late-Mughal south Gujarat was run mostly by Hindu and Jain family firms which operated within a multilingual environment featuring Gujarati and Marathi as well as Persian. Similar arrangements continued under early East India Company control but, by the 1820s, the British had done away with land-revenue family firms and their contextual multilingualism, replacing them with directly-employed village accountants writing only in Gujarati. This article argues that pargana-level officials’ multilingualism and relative autonomy were not an 18th-century aberration but a key feature of Mughal administration, dislodged with difficulty by the British.