bluuebunny/crossref_metadata_2025_split huggingface dataset:
CONTROL AND OPERATIONS MANAGEMENT OF EXTRACTION PROCESS IN INDUSTRIAL BIOTECHNOLOGY OF BETA-CAROTINE FROM BLAKESLEA TRISPORA
Authors: Belinska A. , Varankina O. , Bliznjuk O. , Masalitina N. , Krichkovska L. 
Published: November 2021

The technological parameters, namely temperature and duration of β-carotene extraction process from the biomass of filamentous fungus Blakeslea trispora with vegetable oils of various fatty acid compositions and with various contents of natural antioxidants (refined deodorized sunflower, viso-oleic sunflower, corn and sesame) have been investigated. Statistical models of dependences of β-carotene, as well as analytical numbers, characterizing the content of free fatty acids (acid number) and primary products of lipid oxidation (peroxide number) content, in oil extracts of the specified refined deodorized oils, from temperature and extraction duration have been built.
Rational parameters of β-carotene extraction from Blakeslea trispora biomass with selected extractants (refined deodorized sunflower, high oleic sunflower, corn and sesame oils) have been determined for the extracts technological properties control. It has been proven that the use of these refined deodorized oils as extractants practically does not affect the content of the target product in oil extracts of biomass, but it does affect the analytical numbers of extracts characterizing the content of free fatty acids, peroxides and hydroperoxides. The highest content of free fatty acids in β-carotene containing biomass extraction with sunflower oil has been observed. The minimum content of free fatty acids in extracts with corn and sesame oils using has been be achieved. The highest content of primary products of lipid oxidation (peroxides and hydroperoxides) during β-carotene containing biomass extraction by sunflower oil has been observed. The minimum content of free fatty acids in extracts with sesame oil using has been achieved.
It is possible to predict β-carotene content, as well as acid and peroxide numbers of oil extracts from biomass in specified refined deodorized oils, depending on temperature and extraction process duration using the obtained approximation dependences.
Investigation of Increasing the Tooth Surface Quality of Curvilinear Involute Gears
Authors: uzun mahir , TEMİZ Şemsettin , ÇETİN Mehmet Sinan 
Published: November 2021

Abstract
It is not possible to increase the surface quality of non-standard gears with curved involute tooth profile produced by 5-axis CNC milling method with standard gear grinding methods due to curved tooth profiles. In this study, the possibilities of increasing the surface quality of non-standard gears with curved involute tooth profile were investigated in order to expand their use in the industry. For this purpose, the target model was produced by using the mathematical parametric equations of the involute curve that forms the profile form of a tooth in the CAD environment. By using the target model, manufacturing codes were derived in the CAM environment, and gears with curved involute tooth profile were produced on a 5-axis CNC machine. Then these gears; The possibilities of increasing the tooth profile surface quality were investigated by applying four different methods, such as precision finishing, co-running in oil, co-running in oil with SiC added, and grinding on a 5-axis CNC machine with a finger grinding tip specially produced for gears. In the oil co-run method, considering the running-in stage for each method, the gears were tested with the help of the designed gearbox, at a revolution speed of 670 rpm, by turning a total of 150,000 turns in 6 periods of 25,000 turns. In the co-start method, the gears at the beginning of the test and at the end of each period; thermal records, noise analysis, photographic records, surface roughness values (Ra, Rz) were measured. In the co-operation methods applied to increase the surface quality, the tooth surface of the gears at the beginning of the test and at the end of each period; photographic records, surface roughness values (Ra, Rz) were measured, noise levels were measured during the test and thermal records were taken. Similarly, in precision machining and grinding methods, photographic recordings of the tooth surface were taken, surface roughness (Ra, Rz) was measured, micrographs were taken in SEM and analyzed after the process. As a result of the tests and analyzes carried out; It has been observed that the surface quality is the best in the process performed using a specially produced finger grinding tip and a CNC grinding machine.
A Fine Spatial Resolution Modeling of Urban Carbon Emissions: A Case Study of Shanghai, China
Authors: Huang Cheng , Zhuang Qianlai , Meng Xing , Zhu Peng , Han Ji , Huang Lingfang 
Published: November 2021

Abstract
Quantification of fossil fuel carbon dioxide emissions (CEs) at fine space and time resolution is a critical need in climate change research and carbon cycle. Quantifying changes in spatiotemporal patterns of urban CEs is important to understand carbon cycle and development carbon reduction strategies. However, fine resolution spatial data of CEs deficient. This study quantified CEs from 15 types of energy sources, including residential, tertiary, and industrial sectors in Shanghai. Additionally, we mapped the CEs for the three sectors using point of interest data and web crawler technology, which is different from traditional methods. At a resolution of 30 m, the improved CEs data has a higher spatial resolution than existing studies. In addition, the method of this study is better than traditional methods with details and heterogeneity of CEs, the CEs distribution close to reality. The results indicated that there was a consistent increase in CEs during 2000–2015, with a low rate of increase during 2009–2015. The intensity of CEs increased significantly in the outskirts of the city, mainly due to industrial transfer. Moreover, intensity of CEs reduced in city center. Decoupling between the economic development and CEs in the city was observed during in 2000–2015 owing to technological progress and reduction in CEs intensity.
A locally Active Discrete Memristor Model and Its Application in a Hyperchaotic Map
Authors: Ma Minglin , Yang Yang , Qiu Zhicheng , Peng Yuexi , Sun Yichuang , Li Zhijun , Wang Mengjiao 
Published: November 2021

Abstract
The continuous memristor is a popular topic of research in recent years, however, there is rare discussion about the discrete memristor model, especially the locally active discrete memristor model. This paper proposes a locally active discrete memristor model for the first time and proves the three fingerprints characteristics of this model according to the definition of generalized memristor. A novel hyperchaotic map is constructed by coupling the discrete memristor with a two-dimensional generalized square map. The dynamical behaviors are analyzed with attractor phase diagram, bifurcation diagram, Lyapunov exponent spectrum, and dynamic behavior distribution diagram. Numerical simulation analysis shows that there is significant improvement in the hyperchaotic area, the quasi-periodic area and the chaotic complexity of the two-dimensional map when applying the locally active discrete memristor. In addition, antimonotonicity and transient chaos behaviors of system are reported. In particular, the coexisting attractors can be observed in this discrete memristive system, resulting from the different initial values of the memristor. Results of theoretical analysis are well verified with hardware experimental measurements. This paper lays a great foundation for future analysis and engineering application of the discrete memristor and relevant the study of other hyperchaotic maps.
The Insulin Sensitivity Mcauley Index (Mcai) is Associated with 40-Year Cancer Mortality in a Cohort of Men and Women Free of Diabetes at Baseline
Authors: Moshkovits Yonatan , Rott David , Chetrit Angela , Dankner Rachel 
Published: November 2021

Abstract
Background:The association between insulin resistance and cancer mortality is not fully explored. We investigated the association between several insulin sensitivity indices (ISIs) and cancer mortality in a cohort of adult men and women free of diabetes. We hypothesized that higher insulin resistance (Q1 of the Mcauley index (MCAi), calculated by fasting insulin and triglycerides, and Q4 of the Homeostatic Model Assessment (HOMA), calculated by fasting plasma glucose and insulin) will be associated with greater cancer mortality risk.Methods: A cohort of 1612 men and women free of diabetes during baseline were followed since 1979 through 2016 for cause specific mortality as part of the Israel study on Glucose Intolerance, Obesity and Hypertension (GOH). Results: Mean age at baseline was 51.5 ± 8.0 years, 804 (49.9%) were males, and 871 (54.0%) had prediabetes. Mean follow-up was 36.7±0.2 years and 47,191 person years were accrued. Cumulative incidence analysis using Cox proportional hazard model and competing risks analysis adjusted for age, sex, country of origin, BMI, blood pressure, total cholesterol, smoking and glycemic status (table 2), revealed an increased risk for cancer death, sub-distribution HR=1.4 (95% CI: 1.1-1.9, p=0.02) for individuals in the lower quartile of MCAi (Q1), denoting higher insulin resistance, compared with the upper quartiles (Q2-4). No statistically significant association was observed between the other insulin resistance surrogates and cancer death.Conclusion: The MCAi was found to independently associate with an increased risk for cancer mortality in adult men and women free of diabetes. The MCAi may be considered as a long-term prognostic biomarker in diabetes-free adults.
An Exploratory Analysis of Forme Fruste Keratoconus Sensitivity Diagnostic Parameters
Authors: Zhang Hui , Zhang Xue , Hua Lin , Li Lin , Tian Lei , Zhang Xinxin , Zhang Haixia 
Published: November 2021

Abstract
Purpose: To re-statistical analysis of the Pentacam or Corvis ST parameters from literatures, and to obtain more sensitive diagnostic parameters for clinical keratoconus (CKC) and forme fruste keratoconus (FFKC), respectively. Methods: The parameters and the corresponding area of ROC curve (AUC) in previous studies were extracted and screened to obtain the database of CKC (Data-CKC) and FFKC (Data-FFKC), respectively. Two different importance evaluation methods (%IncMSE and IncNodePurity) of random forest were used to preliminary select the important parameters. Then, based on the partial dependency analysis, the sensitive diagnostic parameters that had promotion to the diagnostic performance were obtained. Data-FFKC was analyzed in the same way. Finally, a diagnostic test meta-analysis on the sensitive parameter of interest was conducted to verify the reliability of the above analysis methods.Results: There were 88 parameters with 766 records in Data-CKC, 57 parameters with 346 records in Data-FFKC. Based on two importance evaluation methods, 60 important parameters were obtained, of which 20 were further screened as sensitive parameters of keratoconus, and most of these parameters were related to the thinnest point of cornea. The stiffness parameter at first applanation (SPA1) was the only Corvis ST output parameter sensitive to FFKC except the Tomographic and Biomechanical Index (TBI) and the Corvis Biomechanical Parameter (CBI). A total of 4 records were included in the meta-analysis of diagnostic tests on SPA1. The results showed that there was threshold effect, but no significant heterogeneity (I2=33%), and the area under the SROC curve was 0.87 (95% CI, 0.84-0.90).Conclusions: the sensitive diagnostic parameters of keratoconus mainly include the 7 categories. The diagnostic value of SPA1, which represents the biomechanical properties of cornea, is worthy of further attention in the diagnosis of keratoconus in forme fruste keratoconus. Moreover, the morphological parameters based on the typical position of the thinnest point of corneal thickness have potential special significance for the diagnosis of disease.
Complement protein C1q is a therapeutic target for neuropathic pain
Authors: Yousefpour Noosha , Tansley Shannon , Locke Samantha , Sharif Behrang , Parisien Marc , Deamond Haley , Mathur Vidhu , Yednock Ted , Austin Jean-Sebastien , Bourassa Valerie , Wang Chengyang , Cabana Valérie , Wong Calvin , Lister Kevin , St-Louis Manon , Andrews-Zwilling Yaisa , Séguéla Philippe , Mogil Jeffrey , De Koninck Yves , Diatchenko Luda , Khoutorsky Arkady , Ribeiro-da-Silva Alfredo 
Published: November 2021

Abstract
Activation of spinal microglia following peripheral nerve injury is a central component of neuropathic pain pathology. While the contributions of microglia-mediated immune and neurotrophic signalling have been well-characterized, the phagocytic and synaptic pruning roles of microglia in neuropathic pain remain unknown. Here, we show that peripheral nerve injury induces engulfment of dorsal horn synapses by microglia, leading to a preferential loss of inhibitory synapses. This synapse removal is dependent on the microglial complement-mediated synapse pruning pathway, as mice deficient in complement C3 do not exhibit synapse elimination. Furthermore, pharmacological inhibition of the complement protein C1q prevents dorsal horn inhibitory synapse loss and attenuates neuropathic pain. Thus, these results demonstrate that the complement pathway promotes persistent pain hypersensitivity via microglia-mediated engulfment and loss of inhibitory synapses in the dorsal horn of the spinal cord, revealing C1q as a novel therapeutic target in neuropathic pain.
Accuracy of CT Evaluation for Cervical Spine Clearance in the Ground Level Fall Population – A Retrospective Cohort Study
Authors: Culhane John , Parr Alan , Mercier Philippe 
Published: November 2021

Abstract
BackgroundClinically occult cervical spine (CS) injuries are well described in blunt trauma, however delay in identifying these injuries and clearing the CS can result in morbidity. Our study examines the ground level fall (GLF) population to analyze whether computed tomography (CT) alone can rule out unstable injury in this group with lower force mechanism.MethodsThis is a single center, retrospective cohort study. Cases of GLF between 6/1/2012 through 12/31/2019 were queried. Characteristics of GLF and non-GLF patients were compared. Significance for categorical data was calculated with Chi Square, Student’s t-test for continuous data, and Mann-Whitney U test for ordinal data. CT negative CS injuries were identified. Binary classification tests were calculated for the ability of CT to identify CS injury using a generalized linear regression model with binomial distribution and identity link.Results69 (2.0%) of patients had CS injury without acute CT abnormality. Of these, 11 (0.3%) required surgery and were considered unstable. All patients who required surgery had a neurologic deficit. Negative predictive value (NPV) of CT for unstable CS injury was 99.7%. The combination of acute CT findings and neurologic deficit ruled out unstable CS injury with 100% NPV.ConclusionIn the GLF population, CT alone rules out unstable CS injury with high, but not perfect NPV. The combination of absence of acute CT findings and acute neurologic deficits rules out unstable CS injury with 100% NPV.
A Fatal Case of Streptococcal Toxic Shock Syndrome Caused By Genotype Emm1 In The Third Trimester of Pregnancy, China
Authors: Luo Yasha , Zheng Minling , Chen Yanyuan , Gu Chunming , Lv Lijuan , Mu Xiaoping 
Published: November 2021

Abstract
Background: Group A streptococcal (GAS) toxic shock syndrome (TSS) is a rare invasive disease, causing a high risk of maternal and fetal mortality during pregnancy. We report a fatal case of a female caused by GAS-TSS in the third trimester of pregnancy in Guangzhou, China. Case presentation: The patient is a 33-year-old female who presented at 37 weeks’ gestation with a history of three hours fever. The patient underwent an early onset and rapid progression with dramatic clinical picture and laboratory characters within 24 hours. The neonate survived after an aggressive anti-infection treatment.The GAS strains were isolated from two bottles of blood cultures and airway secretion culture, which confirmed as Streptococcus pyogenes associated with genotype emm1 by molecular analysis.Conclusion: Dramatic clinical picture and laboratory characters of the pregnant woman presented here might help improve clinicians' awareness and recognition of Streptococcus pyogenes, which could be of great importance for the early diagnosis of GAS- TSS in pregnancy.
Optoelectronic and Birefringence Properties of Weakly Mg Doped ZnO Thin films Prepared By Spray Pyrolysis.
Authors: Bouachibaa Yassine , MAMMERI ABDELOUADOUD , Bouabellou Abderrahmane , Oualid Rabia , Saidi Saber , Taabouche Adel , Rahal Badis , Benharrat Lyes , Serrar Hacene , Boudissa Moukhtar 
Published: November 2021

Abstract
MgxZn1−xO thin films were deposited on glass substrate with x varied between 0.01 and 0.05 by spray pyrolysis at a temperature of 450 °C. The structural investigation showed that all thin films had ZnO wurtzite structure with a preferred (002) orientation. The gap energy was calculated using Tauc’s plot and it decreased over the Mg content by 0.07 eV. The charge carriers’ density dropped by an order of 105 as Mg content increased whereas the resistivity and the mobility increased. SEM observations revealed a significant difference between undoped and doped thin films. A 632.8 nm laser source prism coupler revealed 2 optical modes for every thin film in each Transverse Electric and Transverse Magnetic mode, the birefringence of the Mg doped films was positive. Both ordinary and extraordinary refractive indexes were found to decrease as the Mg content increased. Great intention has been paid to the relation between the refractive, charge carriers density and the optical band gap.
Bioinformatics-Based Study to Investigate Potential Differentially Expressed Genes and miRNAs in Hashimoto’s Thyroiditis
Authors: Guoquan Li , Junwei Du , Qi He , Xinghao Fu , Feihong Ji , Ming Gao , Xinguang Qiu 
Published: November 2021

Abstract
BackgroundHashimoto's thyroiditis (HT), also known as chronic lymphocytic thyroiditis, is a common autoimmune disease, which mainly occurs in women. The early manifestation was hyperthyroidism, however, hypothyroidism may occur if HT was not controlled for a long time. Numerous studies have shown that multiple factors, including genetic, environmental, and autoimmune factors, were involved in the pathogenesis of the disease, but the exact mechanisms were not yet clear. The aim of this study was to identify differentially expressed genes (DEGs) by comprehensive analysis and to provide specific insights into HT. MethodsTwo gene expression profiles (GSE6339, GSE138198) about HT were downloaded from the Gene Expression Omnibus (GEO) database. The DEGs were assessed between the HT and normal groups using the GEO2R. The DEGs were then sent to the Gene Ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis. The hub genes were discovered using Cytoscape and CytoHubba. Finally, NetworkAnalyst was utilized to create the hub genes' targeted microRNAs (miRNAs). ResultsA total of 62 DEGs were discovered, including 60 up-regulated and 2 down-regulated DEGs. The signaling pathways were mainly engaged in cytokine interaction and cytotoxicity, and the DEGs were mostly enriched in immunological and inflammatory responses. IL2RA, CXCL9, IL10RA, CCL3, CCL4, CCL2, STAT1, CD4, CSF1R, and ITGAX were chosen as hub genes based on the results of the protein-protein interaction (PPI) network and CytoHubba. Five miRNAs, including mir-24-3p, mir-223-3p, mir-155-5p, mir-34a-5p, mir-26b-5p, and mir-6499-3p, were suggested as likely important miRNAs in HT. ConclusionsThese hub genes, pathways and miRNAs contribute to a better understanding of the pathophysiology of HT and offer potential treatment options for HT.
Antitumor activity of gambogic acid lysinate in human SiHa cells in vitro and in vivo
Authors: Niu Jie , Sun Jingyu , Liu Yahua , Guo Jun , Zhang Xin , Liu Yinghua , Lin Yajun 
Published: November 2021

Abstract
Background: Cervical cancer is a major cause of death for women worldwide and human papillomavirus (HPV) infection is the main cause of cervical cancer. The purpose of this study was to explore the anti-tumor activity of gambogic acid lysinate and clarify its mechanism in SiHa cells. Methods: In the present study, cell viability was detected by means of an MTT assay, a cell growth curve was drawn with Microsoft Excel 2010, the cell cycle and cell apoptosis were evaluated by flow cytometry, Western blotting was employed to explore the mechanism of gambogic acid lysinate, and caspase-3 activity was determined with a colorimetric Caspase-3 assay kit. Additionally, the in vivo antitumor activity of gambogic acid lysinate was studied through a xenograft tumor model established with nude mice. Results: The results showed that gambogic acid lysinate inhibited the proliferation of both SiHa cells (half-maximal inhibitory concentration (IC50) values: 0.83 μmol/l and 0.77 μmol/l for 48 h and 72 h) and HeLa cells (IC50 >2 μmol/l). In SiHa cells, gambogic acid lysinate (1 and 2 μmol/l) inhibited cell proliferation and 2 μmol/l gambogic acid lysinate induced cell apoptosis and decreased the number of S phase cells. Both 1 and 2 μmol/l gambogic acid lysinate increased the number of G0/G1 phase cells. The results of a Western blot assay demonstrated that P53 and P21 were involved in SiHa cell G0/G1 phase arrest and that Bcl-2 and BAX were involved in SiHa cell apoptosis. An in vivo study showed that the growth of SiHa cell xenograft tumors was inhibited by gambogic acid lysinate (2.5 mg/kg body weight), however, gambogic acid lysinate (2.5 mg/kg body weight) had no significant effect on mouse weight gain. Conclusions: gambogic acid lysinate is a promising candidate for cervical cancer therapy.
Prognostic Significance of Thyroid Hormone For Prolonged Mechanical Ventilation In Critically Ill Patients With Cardiac Surgery
Authors: Shen Xiao , Sun Jiakui , Hong Liang , Song Xiaochun , Zhang Cui , Liu Ying , Liu Han , Mu Xinwei 
Published: November 2021

Abstract
Background: This study aimed to examine the correlation between thyroid hormone and prolonged mechanical ventilation (MV) in the adult critically ill patients having undergone cardiac surgery. Methods: The present study refers to a retrospective, cohort study that was conducted at Cardiovascular Intensive Care Unit (CVICU) of Nanjing First Hospital from March 2019 to December 2020. Patients receiving cardiac surgery and admitted to the center of the authors in the study period were screen for a potential inclusion. Demographic information, thyroid hormone and other laboratory measurements and outcome variables were recorded for analyses. Prolonged MV was defined as the duration of MV after cardiac surgery longer than 5 days. Thyroid hormones were assessed for the prognostic significance for prolonged MV.Results: On the whole, 118 patients having undergone cardiac surgery were included and analyzed in this study. Patients fell to the control (n=64) and the prolonged MV group (n=54) by complying with the duration of MV after cardiac surgery. The median total triiodothyronine (TT3) and free triiodothyronine (FT3) were 1.03 nmol/L and 3.52 pmol/L in the prolonged MV group before cardiac surgery, significantly lower than 1.23 nmol/L (P=0.005) and 3.87 pmol/L, respectively in the control (P=0.038). multivariate logistic regression analysis indicated that TT3 before surgery (pre-op TT3) had a good prognostic significance for prolonged MV (OR: 0.049, P=0.012). Conclusions: This study concluded that decreased triiodothyronine (T3) could be common in the cardiac patients with prolonged MV, and it would be further reduced after patients undergoing cardiac surgery. Besides, decreased T3 before surgery could act as an effective predictor for prolonged MV after cardiac surgery.