bluuebunny/crossref_metadata_2025_split huggingface dataset:
Chiral voltammetric sensor for tryptophan enantiomers by using a self‐assembled multiwalled carbon nanotubes/polyaniline/sodium alginate composite
Authors: Niu Xiaohui , Yang Xing , Li Hongxia , Shi Qiuyun , Wang Kunjie 
Published: March 2021

AbstractDue to the crucial role of amino acids in life sciences and pharmaceutics, identification of optical amino acid molecules is of great significance. In this study, the two materials (CNT and PANI) were combined together to obtain the magnification of electrochemical signal by substrate material (CNT/PANI). Then a self‐assembled multiwalled carbon nanotubes/polyaniline/sodium alginate (CNT/PANI/SA) nanocomposite with chiral sites and conductive material was synthesized as the electrochemical sensing interface. Next, a novel electrochemical sensing interface was fabricated via modifying the as‐prepared chiral material on a polished glassy carbon electrode (CNT/PANI/SA/GCE) for precisely, efficiently, and rapidly differentiation of tryptophan (Trp) enantiomers. It was observed that CNT/PANI/SA/GCE showed desirable stereoselective recognition effect in the variety of signal strength to peak current (Ip) to the different optical activity of Trp enantiomers. In the case of optimal conditions, the peak current ratio in the solution of l‐Trp and d‐Trp (ID/IL) was observed to be 2.1 at CNT/PANI/SA/GCE by differential pulse voltammogram (DPV). UV–visible spectroscopy further showed that CNT/PANI/SA had a greater binding energy to l‐Trp. Also different factors affecting the enantioselectivity of CNT/PANI/SA/GCE, such as the incubation time, pH, and dropcoating volume of CNT/PANI/SA were optimized. Moreover, the proposed CNT/PANI/SA/GCE showed excellent specific stereoselectivity and anti‐interference ability. Besides, the proposed chiral sensing platform can be effectively applied in real samples to detect Trp enantiomers sensitively. This work inspires us a new path for the preparation of substrate material with excellent electrical conductivity, as well as extend its application potential in chiral recognition.
Combining Metabolic Alkyne Labeling and Click Chemistry for Secretome Analysis of Serum‐Containing Conditioned Medium†
Authors: Zheng Jiangnan , Mao Yuan , Feng Shun , Tian Ruijun 
Published: March 2021

Main observation and conclusionBioorthogonal click chemistry has emerged as a powerful tool for the specific modification of proteins in complex mixtures. Metabolic labeling of proteins with azide followed by the copper‐catalyzed azide−alkyne cycloaddition (CuAAC) with alkyne‐based affinity probes/beads is widely applied to study protein turnover and post‐translational modifications (PTMs). However, it has long been known that the alkyne‐based enrichment of high concentration protein samples (e.g., serum) is not sufficient to remove high abundant contaminating proteins. Herein, we demonstrate that the protein contamination is mainly caused by thiol‐yne addition between terminal alkyne and cysteine residue. Furthermore, we report that azide‐based enrichment combined with metabolic labeling with alkyne can significantly reduce contamination and improve the enrichment efficiency of secreted proteins in serum‐containing conditioned medium.
Effects of density on colour and gloss variability changes of wood induced by heat treatment
Authors: Zhou Fan , Zhou Yongdong , Fu Zongying , Gao Xin 
Published: March 2021

AbstractThe influence of heat treatment severity (resulting from temperature) on the colour and gloss distribution changes of mahogany wood, and the effects of wood density on the responses to heat treatment were evaluated. The wood timbers with different densities were treated at 150°C to 200°C with an interval of 10°C and duration of 4 hours for heat treatment schedules. The colour parameters (lightnessL*, red‐green axis valuea*, yellow‐blue axis valueb*) and gloss (parallel and perpendicular to the grain) values of heat‐treated wood were determined. The distributions of colour and gloss were analyzed using a frequency counts method. Results show that heat treatment resulted in the distinct decrease inL*andb*, while almost no change ina*for mahogany wood. The lower gloss values were obtained in the wood after heat treatment. The colour and gloss uniform on the exposed surface of the wood was improved due to heat treatment. Heat‐treatment induced colour and gloss changes were more effective in the samples with lower density. The effects of density on the colour and gloss variability in the original wood were reduced by heat treatment, and this reduction was more distinct at high heat treatment temperature. The total gloss change was found to be highly correlated with the total colour change of heat‐treated wood.
The colour of emerging textile for urban regeneration: the role of technology between experimentations and craftsmanship
Authors: Gasparini Katia 
Published: March 2021

AbstractThis article aims to study the role of coloured installation in the urban regeneration processes. The urban colour designs are fundamental for many reasons: the environmental quality and the human wellbeing, the space valorization and the wayfinding. In this focus the colour, the material and the interaction play an important role. Some of most interesting installations in the world are made by generative design and emerging technologies, in a multidisciplinary design approach. The research methodology is based on the analysis of international case studies about interactive coloured installations, to define the role of colour in relation with the urban scene. The results seem to show that emerging technologies are still hardly applied to urban installations and the role of the colour is fundamental, in particular the one of warm colours or multicolour installations.
Biogeographic position and body size jointly set lower thermal limits of wandering spiders
Authors: Monsimet Jérémy , Colinet Hervé , Devineau Olivier , Lafage Denis , Pétillon Julien 
Published: March 2021

AbstractMost species encounter large variations in abiotic conditions along their distribution range. The physiological responses of most terrestrial ectotherms (such as insects and spiders) to clinal gradients of climate, and in particular gradients of temperature, can be the product of both phenotypic plasticity and local adaptation. This study aimed to determine how the biogeographic position of populations and the body size of individuals set the limits of cold (freezing) resistance ofDolomedes fimbriatus. We comparedD. fimbriatusto its sister speciesDolomedes plantariusunder harsher climatic conditions in their distribution range. Using an ad hoc design, we sampled individuals from four populations ofDolomedes fimbriatusoriginating from contrasting climatic areas (temperate and continental climate) and one population of the sister speciesD. plantariusfrom continental climate, and compared their supercooling ability as an indicator of cold resistance. Results forD. fimbriatusindicated that spiders from northern (continental) populations had higher cold resistance than spiders from southern (temperate) populations. Larger spiders had a lower supercooling ability in northern populations. The red‐listed and rarestD. plantariuswas slightly less cold tolerant than the more commonD. fimbriatus, and this might be of importance in a context of climate change that could imply colder overwintering habitats in the north due to reduced snow cover protection. The lowest cold resistance might putD. plantariusat risk of extinction in the future, and this should be considered in conservation plan.
A pilot study assessing the brain gauge as an indicator of cognitive recovery in alcohol dependence
Authors: Powell Anna , Tommerdahl Mark , Abbasi Yasir , Sumnall Harry , Montgomery Catharine 
Published: March 2021

AbstractAlcohol dependence (AD) is associated with multiple cognitive deficits, which can affect treatment outcomes. Current measures of tracking brain recovery (e.g., functional magnetic resonance imaging) can be less accessible for practitioners. This study pilots a novel device (the brain gauge; BG) to assess its utility, and track recovery of cognitive function in residential alcohol treatment. Methods: A repeated measures design assessed changes in cognitive function during detoxification. Twenty‐one participants with AD (16 Male; Mean age 43.85 ± 6.21) completed a battery of alcohol and memory questionnaires and BG tasks at two time‐points (∼days 4 and 10) during a single managed detoxification episode. Results: Repeated measures ANCOVA revealed that some BG metrics significantly improved, with medium to large effect sizes ‐ processing speed, focus, temporal order judgement and overall cortical metric. However, differences in subjective cognitive function were non‐significant after controlling for depression and anxiety change scores. Anxiety change emerged as a significant factor in subjective cognitive function. Conclusions: We conclude it is possible that the prefrontal cortex (PFC) recovers more slowly compared to other brain areas, and there are compounding effects of improvements in anxiety and depression, and metacognitive deficits on subjective EF assessments. Future research should seek to validate the clinical utility of the BG by comparing against established neuroimaging methods.
Seasonal changes in depth position and temperature of European catfish (Silurus glanis) tracked by acoustic telemetry in the Danube River
Authors: Lenhardt Mirjana B. , Smederevac‐Lalić Marija M. , Spasić Sladjana Z. , Honţ Ştefan , Paraschiv Marian , Iani Marian I. , Nikčević Miroslav V. , Klimley Peter A. , Suciu Radu 
Published: March 2021

AbstractThe giant European catfish, Silurus glanis (total length = 200 cm; total weight ≈ 80 kg) was caught downstream of Iron Gate II hydropower dam (Danube River, 863 rkm) and tagged with an ultrasonic transmitter (V16TP; Vemco Ltd.) equipped with depth and temperature sensors. Changes in catfish diving behavior and temperature exposure were monitored over a period of roughly 2 years. Transmitter detections were recorded by nine autonomous receivers (VR2W, installed in 2015 between Serbia and Romania, as well as near Romanian shiplock and upstream Romanian turbines). The first signals were recorded on April 28, 2015 and the last on February 13, 2017. Altogether 59,355 and 59,175 detections of the catfish depth and water temperature were recorded, respectively. The greatest number of signals were recorded by the two receivers closest to the location where the catfish was caught, 72.3% and 27.1%, while only 0.6% of signals were recorded by other receivers. The mean catfish depth was 8.4 m, while minimum and maximum depths were 1.2 and 16.2 m. Results obtained showed that this catfish exhibited high site fidelity, while changes in depth at certain periods are possibly related to its search for prey and upstream migration during the spawning period. Hydropower dam and shiplock were obstacles on its migration upstream and telemetry studies could ensure habitat requirements and meet the development of restoration and conservation strategies for the fish resources in the future.
Plerixafor added to G‐CSF allows mobilization of a sufficient number of hematopoietic progenitors without impacting the efficacy of TCR‐alpha/beta depletion in pediatric haploidentical and genoidentical donors failing to mobilize with G‐CSF alone
Authors: Kurnikova Elena , Trakhtman Pavel , Pershin Dmitry , Ilyushina Mariya , Khismatullina Rimma , Maschan Michael , Novichkova Galina , Maschan Alexey 
Published: March 2021

AbstractBackgroundCollection of a large number of early hematopoietic progenitors is essential for allogeneic apheresis products intended for TCR‐alpha/beta depletion.Materials and MethodsWe added plerixafor 0.24 mg/kg body weight (bw) on day 4 of high‐dose filgrastim mobilization 10 hours prior to apheresis in 16 (30.5%) pediatric allogeneic donors who failed to recover a sufficient number of CD34+ cells.ResultsOn day 4 of G‐CSF, the median CD34+ cell count in peripheral blood was 6 per μL (range 4‐9 per μL) in 6 poor mobilizers and 16 per μL (range 12‐19 per μL) in insufficient mobilizers. In all donors, the threshold of 50 CD34+ cells/μL was achieved, and the median increase was 14.8‐fold in poor mobilizers and 6.5‐fold in insufficient mobilizers, whereas it was 3.45‐fold increase in those mobilized with G‐CSF alone.DiscussionIn all donors, a predefined number of >10 × 106 CD34+ cells/kg of recipient bw before depletion was reached in the apheresis product. The use of plerixafor did not affect the purity of further TCR‐alpha/beta depletion. Side effects were mild to moderate and consisted of nausea and vomiting.ConclusionThus, the safety and high efficacy of plerixafor was proven in healthy pediatric allogeneic hematopoietic cell donors.
The explicit role of electron exchange in the hydrogen bonded molecular complexes
Authors: Levina Elena O. , Khrenova Maria G. , Tsirelson Vladimir G. 
Published: March 2021

AbstractWe applied a set of advanced bonding descriptors to establish the hidden electron density features and binding energy characteristics of intermolecular DH∙∙∙A hydrogen bonds (OH∙∙∙O, NH∙∙∙O and SH∙∙∙O) in 150 isolated and solvated molecular complexes. The exchange‐correlation and Pauli potentials as well as corresponding local one‐electron forces allowed us to explicitly ascertain how electron exchange defines the bonding picture in the proximity of the H‐bond critical point. The electron density features of DH∙∙∙A interaction are governed by alterations in the electron localization in the H‐bond region displaying itself in the exchange hole. At that, they do not depend on the variations in the exchange hole mobility. The electrostatic interaction mainly defines the energy of H‐bonds of different types, whereas the strengthening/weakening of H‐bonds in complexes with varying substituents depends on the barrier height of the exchange potential near the bond critical point. Energy variations between H‐bonds in isolated and solvated systems are also caused the electron exchange peculiarities as follows from the corresponding potential and the interacting quantum atom analyses complemented by electron delocalization index calculations. Our approach is based on the bonding descriptors associated with the characteristics of the observable electron density and can be recommended for in‐depth studies of non‐covalent bonding.
Performance evaluation of a lateral flow assay for nasopharyngeal antigen detection for SARS‐CoV‐2 diagnosis
Authors: Peña‐Rodríguez Marcela , Viera‐Segura Oliver , García‐Chagollán Mariel , Zepeda‐Nuño José Sergio , Muñoz‐Valle José Francisco , Mora‐Mora Jesús , Espinoza‐De León Gabriela , Bustillo‐Armendáriz Gustavo , García‐Cedillo Fernanda , Vega‐Magaña Natali 
Published: March 2021

AbstractBackgroundSARS‐CoV‐2 has become a global pandemic due to its capacity for rapid transmission. In this context, an early and rapid diagnosis of infected patients that do not require expensive equipment or highly trained personnel is crucial in order to reduce the contagious rate. The aim of this study was to evaluate a chromatographic immunoassay's performance for the rapid diagnosis of SARS‐CoV‐antigen.MethodsA cross‐sectional study included 369 adults from Western México with diagnosis or suspicion of SARS‐CoV‐2 infection. Two samples were collected; a naso‐oropharyngeal was used for a molecular determination of SARS‐CoV‐2 RNA. The molecular analysis was carried out using DeCoV19 Kit Triplex (Genes2life S.A.P.I.) based on the CDC diagnostic panel for N1, N2, and N3 regions. The second sample was retrieved from a nasopharyngeal rub and used for the rapid diagnosis of SARS‐CoV‐2 antigen employing the commercial STANDARD™ Q COVID‐19 Ag Test (SD BIOSENSOR).ResultsOverall, in 28.2% of the patients was detected the SARS‐CoV‐2 RNA, and 21.4% were positive for antigen detection. The rapid antigen test showed a sensitivity and specificity of 75.9% and 100%, respectively, with a positive predictive and negative values of 100% and 91%. Symptoms as anosmia presented a high OR for the positive diagnosis for both test, reverse transcription‐polymerase chain reaction (RT‐PCR), and the rapid antigen test of 8.86 (CI = 4.91–16) and 6.09 (CI = 3.42–10.85), respectively.ConclusionSD BIOSENSOR is a useful assay, but some caveats must be considered before the general implementation.
Morphology of ctenostome bryozoans: 4. Pierrella plicata
Authors: Schwaha Thomas , Grischenko Andrei V. , Melnik Viacheslav P. 
Published: March 2021

AbstractThe genus Pierrella was originally created for a single fossil ctenostome bryozoan species from the Late Cretaceous, which is characterized by runner‐like colonies, with zooids possessing a distinctive radial, folded aperture. Not long ago, a few specimens of a recent deep‐sea congener, Pierrella plicata, were discovered and described from the Russian exploration area of the Clarion‐Clipperton Fracture Zone, eastern Central Pacific Ocean. Owing to the lack of data on the internal morphology of this species, we investigated the soft‐body morphology of P. plicata using serial sectioning and 3D‐reconstruction in order to compare it to other more recently investigated ctenostome bryozoans and to infer the systematic position of the genus. The most striking peculiarity of the examined species is the radial aperture formed by multiple cuticular, pleated folds of the cystid wall. The cuticle is thickened into triangular‐shaped folds in this area. An orifical sphincter underlies the folded aperture. Apertural muscles are present as a single pair of parieto‐diaphragmatic muscles and four duplicature bands. The remaining polypide anatomy is mainly characterized by its miniature design: the lophophore has eight short tentacles and the digestive tract is one of the shortest and most compact ever observed in any bryozoan. A small intertentacular organ was detected at the lophophoral base. Taken together the genus Pierrella shows unique characters, such as the radial apertural folds that are closed by a series of orificial sphincter muscles, and its particularly small polypide. The general colony morphology resembles arachnidioidean ctenostomes whereas its internal morphology resembles alcyonidioidean species.
Pregnancy and Delivery Outcomes in Women With Persistent Versus Resolved Low‐Lying Placenta in the Late Third Trimester
Authors: Ornaghi Sara , Vaglio Tessitore Isadora , Vergani Patrizia 
Published: March 2021

ObjectivesThe safest mode of delivery in low‐lying placenta is debatable. Little is known about outcomes in low‐lying placenta resolved during the late third trimester.We compare outcomes of women with persistent versus resolved low‐lying placenta.MethodsA retrospective analysis on a prospective cohort of women with low‐lying placenta confirmed at 28–30 weeks sonography (01/2009 to 03/2018). Women were followed up serially every 2 to 3 weeks until delivery to assess the placental edge‐to‐internal os distance (IOD), and included if scan was performed within 28 days before delivery.ResultsThere were 86 women: in 21 the low‐lying placenta resolved, whereas in 65 persisted (n = 15 IOD 1–10 mm, n = 50 IOD 11–20 mm). Antepartum bleeding associated with higher rates of urgent cesarean delivery in 1–10 mm (P = .041) but not in 11–20 mm (P = 1.000) and >20 mm (P = .333). Among women with IOD >10 mm allowed to labor, vaginal delivery occurred in 76.7% (11–20 mm) and 94.1% (>20 mm) (P = .155), with no difference according to parity (70% and 80% in multiparas, P = .696; 60% and 72.7% in nulliparas, P = .698). Severe PPH (P = .922) and hemoglobin drop (P = .109) were similar among groups. Women with IOD 11–20 mm and >20 mm and vaginal delivery bled less than women with similarly located placenta and cesarean delivery (P = .009 and P = .048).ConclusionsWomen with IOD >10 mm have high chances of deliver vaginally with no further increase of their hemorrhagic risk. Success of vaginal delivery is independent of parity and antepartum bleeding. Labor should be managed in an adequate hospital setting.
Silk Film Stiffness Modulates Corneal Epithelial Cell Mechanosignaling
Authors: Sun Michael G. , Luo Yuncin , Teng Tao , Guaiquil Victor , Zhou Qiang , McGinn Lander , Nazzal Osayd , Walsh Michael , Lee James , Rosenblatt Mark I. 
Published: March 2021

AbstractSilk fibroin films are excellent candidate biomaterials for corneal tissue engineering due to their optical transparency, biocompatibility, and mechanical strength. Their tunable chemical and mechanical properties open the possibility of engineering cellular microenvironments that can both mimic native corneal tissue and provide stimuli to actively promote wound regeneration. While silk film mechanical properties, such as surface topography, have demonstrated the ability to control corneal epithelial cell wound regenerating behavior, few studies have explored the stiffness tunability of these films and its cellular effects. Cells are known to actively sense the stiffness of their surroundings and processes such as cell adhesion, migration, proliferation, and expression of stem markers can be strongly influenced by matrix stiffness. This study develops technical solutions that allow for both the fabrication of films with stiffnesses similar to corneal tissue and also for their characterization in an aqueous, native‐like environment at a scale relevant to cellular forces. Physiological evidence demonstrates that corneal epithelial cells are mechanosensitive to films of different stiffnesses and show that cell spreading, cytoskeletal tension, and molecular mechanotransducer localization are associated with film stiffness. These results indicate that silk film stiffness can be used to regulate cell behavior for the purposes of ocular surface repair.
Efficiency and satisfaction with telephone consultation of follow‐up patients in neuro‐urology: Experience of the COVID‐19 pandemic
Authors: Chesnel Camille , Hentzen Claire , Le Breton Frédérique , Turmel Nicolas , Tan Eliane , Haddad Rebecca , Amarenco Gérard 
Published: March 2021

AbstractAimThe COVID‐19 pandemic led to limit patients’ visits to the neuro‐urology department. Telemedicine was seen as a pragmatic solution to provide follow‐up care. This study aimed to assess the efficiency and satisfaction of a telephone consultation in neuro‐urology.MethodsDuring the pandemic, the scheduled medical visits were converted into telephone consultation. For each teleconsultation, the physician assessed the efficiency and the patient‐rated global satisfaction of the teleconsultation. The physician and the patient assessed whether this teleconsultation replaced a physical visit.ResultsAbout 358 neurologic patients were included in the study. The mean efficiency of the telephone consultation was 9.3/10 (±1.5). The mean global satisfaction was 9.0/10 (±1.3). The majority of the patients (52.4%) would prefer a physical consultation. 90.2% might convert some clinic visits to teleconsultations in the future. No agreement was found between the patient and the physician when they were asked if the teleconsultation replaced the physical consultation initially scheduled (weight kappa = 0.02; 95% confidence interval = [−0.06 to 0.11]). Cognitive impairment, difficulty to obtain relevant information, and lack of physical examination were unfavorable to the efficiency of the teleconsultation. Cognitive impairment, embarrassing nature of the teleconsultation, and preference for a physical consultation were unfavorable to satisfaction of the patient.ConclusionTelemedicine in neuro‐urology was associated with a high satisfaction of the patients and was described as efficient by the physicians. Despite this, the majority of the patients reported a preference for physical consultation. The COVID‐19 pandemic might be an opportunity to refine our practices in neuro‐urology and to develop telemedicine.