bluuebunny/crossref_metadata_2025_split huggingface dataset:
Patterns of Nutrient Intake in Relation to Sarcopenia and Its Components
Authors: Bagheri Amir , Hashemi Rezvan , Heshmat Ramin , Motlagh Ahmadreza Dorosty , Esmaillzadeh Ahmad 
Published: April 2021

Background: Despite the associations between individual nutrients and sarcopenia, we are aware of no information about the link between patterns of nutrient intake and odds of sarcopenia and its components. The present study aimed to examine the association between nutrient-based dietary patterns and sarcopenia and its components among the Iranian adult population.Methods: In this population-based, cross-sectional study, we enrolled 300 elderly adults (150 men and 150 women) aged ≥55 years by using a cluster random sampling method. Dietary intakes of the study population were assessed using a validated food frequency questionnaire. Principal component analysis was conducted to derive nutrient patterns based on a daily intake of 33 nutrients. Muscle mass, muscle strength, and gait speed were measured according to standard methods. Sarcopenia and its components were defined based on the European Working Group on Sarcopenia.Results: Three major nutrient-based dietary patterns were identified: (1) the “pro-vit pattern” that was high in pantothenic (B5), cobalamin (B12), calcium, protein, phosphor, riboflavin (B2), zinc, cholesterol, saturated fat, folate, niacin (B3), selenium, vitamin D, vitamin K, and vitamin A; (2) the “anti-inflammatory” pattern, which was rich in polyunsaturated fat, monounsaturated fat, copper, vitamin E, omega-3, magnesium, iron, pyridoxine (B6), sodium, and caffeine; and (3) the “carbo-vit” patternm which is characterized by high intake of fructose, glucose, dietary fiber, biotin, potassium, thiamin (B1), vitamin C, and chromium. After adjusting for confounders, subjects in the top tertile of the anti-inflammatory pattern had lower odds of sarcopenia (OR 0.25; 95% CI 0.10–0.63) and low muscle strength (OR: 0.46; 95% CI: 0.22–0.96) than those in the bottom tertile. Greater adherence to the carbo-vit pattern was inversely associated with the odds of low gait speed (OR: 0.46; 95% CI: 0.235–0.93).Conclusion: Major nutrient-based dietary patterns were significantly associated with sarcopenia and its components. Further studies are required to confirm our findings.
Identification and Validation of SNP-Containing Genes With Prognostic Value in Gastric Cancer via Integrated Bioinformatics Analysis
Authors: Li Hui , Guo Jing , Cheng Guang , Wei Yucheng , Liu Shihai , Qi Yaoyue , Wang Gongjun , Xiao Ruoxi , Qi Weiwei , Qiu Wensheng 
Published: April 2021

BackgroundGastric cancer is one of the most common malignancies worldwide. Although the diagnosis and treatment of this disease have substantially improved in recent years, the five-year survival rate of gastric cancer is still low due to local recurrence and distant metastasis. An in-depth study of the molecular pathogenesis of gastric cancer and related prognostic markers will help improve the quality of life and prognosis of patients with this disease. The purpose of this study was to identify and verify key SNPs in genes with prognostic value for gastric cancer.MethodsSNP-related data from gastric cancer patients were obtained from The Cancer Genome Atlas (TCGA) database, and the functions and pathways of the mutated genes were analyzed using DAVID software. A protein-protein interaction (PPI) network was constructed using the STRING database and visualized by Cytoscape software, and molecular complex detection (MCODE) was used to screen the PPI network to extract important mutated genes. Ten hub genes were identified using cytoHubba, and the expression levels and the prognostic value of the central genes were determined by UALCAN and Kaplan-Meier Plotter. Finally, quantitative PCR and Western blotting were used to verify the expression of the hub genes in gastric cancer cells.ResultsFrom the database, 945 genes with mutations in more than 25 samples were identified. The PPI network had 360 nodes and 1616 edges. Finally, cytoHubba identified six key genes (TP53, HRAS, BRCA1, PIK3CA, AKT1, and SMARCA4), and their expression levels were closely related to the survival rate of gastric cancer patients.ConclusionOur results indicate that TP53, HRAS, BRCA1, PIK3CA, AKT1, and SMARCA4 may be key genes for the development and prognosis of gastric cancer. Our research provides an important bioinformatics foundation and related theoretical foundation for further exploring the molecular pathogenesis of gastric cancer and evaluating the prognosis of patients.
Combination of Urine Exosomal mRNAs and lncRNAs as Novel Diagnostic Biomarkers for Bladder Cancer
Authors: Huang Haiming , Du Jialin , Jin Bo , Pang Lu , Duan Nan , Huang Chenwei , Hou Jiayin , Yu Wei , Hao Han , Li Haixia 
Published: April 2021

BackgroundThe recent discovery of miRNAs and lncRNAs in urine exosomes has emerged as promising diagnostic biomarkers for bladder cancer (BCa). However, mRNAs as the direct products of transcription has not been well evaluated in exosomes as biomarkers for BCa diagnosis. The purpose of this study was to identify tumor progression-related mRNAs and lncRNAs in urine exosomes that could be used for detection of BCa.MethodsRNA-sequencing was performed to identify tumor progression-related biomarkers in three matched superficial tumor and deep infiltrating tumor regions of muscle-invasive bladder cancer (MIBC) specimens, differently expressed mRNAs and lncRNAs were validated in TCGA dataset (n = 391) in the discovery stage. Then candidate RNAs were chosen for evaluation in urine exosomes of a training cohort (10 BCa and 10 healthy controls) and a validation cohort (80 BCa and 80 healthy controls) using RT-qPCR. The diagnostic potential of the candidates were evaluated by receiver operating characteristic (ROC) curves.ResultsRNA sequencing revealed 8 mRNAs and 32 lncRNAs that were significantly upregulated in deep infiltrating tumor region. After validation in TCGA database, 10 markedly dysregulated RNAs were selected for further investigation in urine exosomes, of which five (mRNAs: KLHDC7B, CASP14, and PRSS1; lncRNAs: MIR205HG and GAS5) were verified to be significantly dysregulated. The combination of the five RNAs had the highest AUC to disguising the BCa (0.924, 95% CI, 0.875–0.974) or early stage BCa patients (0.910, 95% CI, 0.850 to 0.971) from HCs. The expression levels of these five RNAs were correlated with tumor stage, grade, and hematuria degrees.ConclusionsThese findings highlight the potential of urine exosomal mRNAs and lncRNAs profiling in the early diagnosis and provide new insights into the molecular mechanisms involved in BCa.
Vagada Sankiraka Sinthamani Olaichuvadiyin Stylistic Features
Authors: Suba T , Aji K 
Published: April 2021

This study examines the songs in the Medical Leaf Manuscript Vagada Sankiraka Sinthamani and Presents their Stylistic elements. This study also provides explanations of Stylistics as well as stylistic elements such as Phonics, Vocabulary selection, and Syntax trends. The study also explores Phonetic, Syllable, Vocabulary, Adjectives, Adverbs, Medical Terms, Nouns, New words, Biological Names, Herbal Names, Disease Names, Metaphorical Name Series and Numeral Series in Syntax.
THE EFFECT OF E-PROCUREMENT SYSTEM IMPLEMENTATION, COMPETENCE AND COMPENSATION ON EMPLOYEE PERFORMANCE OF GOODS/SERVICES PROCUREMENT
Authors: Yuliawati Nenden Maulida , Icih Icih , Kurniawan Asep 
Published: April 2021

This research was conducted to analyze the magnitude of the effect of the partial and simultaneous implementation of the E-Procurement, Competence and Compensation System on the Goods / Services Procurement Employee Performance in Subang Regency.
This research uses quantitative explanatory methods. The population and sample of the study are employees in the New / Service Procurement Unit in Subang Regency, so the total population is 31 people. Data collection methods used in this study are survey methods by distributing questionnaires. Where each respondent is given questions / statements as many as 32 items using a Likert scale. Data is processed using SPSS 22.0 version.
Based on the results of data processing obtained results there is a positive and significant effect for the variable Application of E-Procurement, Competence and Compensation System partially and simultaneously to Employee Procurement of Goods / Services.
Barkoding DNA pada Tumbuhan Durik-Durik (Syzygium sp.) Asal Riau Menggunakan Daerah Gen ndhF
Authors: Roslim Dewi Indriyani , Fitriani Ana 
Published: April 2021

(Article History: Received 11 November 2020; Revised 9 January 2021; Accepted 18 January 2021) ABSTRAKGen ndhF telah digunakan sebagai salah satu barkode DNA pada genus Syzygium karena divergensi urutan asam aminonya lebih besar empat kali dibandingkan rbcL. Penelitian ini bertujuan menganalisis sekuen DNA dari gen ndhF pada durik-durik (Syzygium sp.) asal Riau. Metode penelitian meliputi isolasi DNA total menggunakan kit isolasi DNA (Genomic DNA Mini Kit Plant, Geneaid), PCR, elektroforesis menggunakan 1% gel agarosa, sekuensing dan analisis data menggunakan program BioEdit versi 7, BLASTn dan MEGA 6.0. Sekuen DNA dari gen ndhF durik-durik telah diperoleh dengan ukuran 1370 pb dan memiliki kemiripan paling tinggi dengan yang dimiliki oleh S. malaccense (99,71%) dan paling rendah dengan S. acuminatissimum (98,69%). Terdapat 26 variasi nukleotida di antara aksesi yang diteliti dan dua diantaranya merupakan nukleotida kritis. Durik-durik membentuk satu kelompok dengan S. malaccense dan S. aromaticum. Namun demikian, nama spesies durik-durik belum dapat ditentukan karena tidak ada aksesi yang memiliki kemiripan 100% dengan durik-durik.Kata kunci: Barkode DNA, Danau Kajuik, durik-durik, gen ndhF, Syzygium. ABSTRACTThe ndhF gene has been used as DNA barcode in Syzygium because the divergence of the amino acids sequence is four times greater than rbcL. This study was to analyze the DNA sequence of ndhF gene on durik-durik (Syzygium sp.) origin Riau. Methods included total DNA isolation using Genomic DNA Mini Kit Plant (Geneaid), PCR, electrophoresis using 1% agarose gel, sequencing and data analysis using BioEdit, BLASTn dan MEGA 6.0 programs. The DNA sequence of durik-durik ndhF gene was 1370 bp in size and had the higher similarity to the one of S. malaccense (99.71%) and the lower similarity to the one of S. acuminatissimum (98.69%). There were 26 nucleotide variations and two of them were critical nucleotides. Durik-durik formed one group with S. malaccense dan S. aromaticum. Nevertheless, the species name of durik-durik still unknown because there is no accessions having 100% similarity to durik-durik.Keywords: DNA barcode, durik-durik, Kajuik Lake, ndhF gene, Syzygium.
Keanekaragaman dan Pemanfaatan Tanaman Pekarangan di Desa Taripa, Kecamatan Pamona Timur, Kabupaten Poso, Sulawesi Tengah
Authors: Tobondo Vanda Evanglin , Koneri Roni , Pandiangan Dingse 
Published: April 2021

(Article History: Received January 14, 2021; Revised February 15, 2021; Accepted February 28, 2021) ABSTRAK Pekarangan merupakan sebidang tanah yang di atasnya terdapat bangunan tempat tinggal dan dapat dibudidayakan berbagai spesies tanaman. Penelitian ini bertujuan menganalisis keanekaragaman dan pemanfaatan tanaman pekarangan di Desa Taripa, Kecamatan Pamona Timur, Kabupaten Poso, Sulawesi Tengah. Pengambilan sampel berdasarkan luas pekarangan yaitu kategori sempit (100-200 m2), sedang (300-400 m2) dan luas (500-600 m2). Pada setiap kategori diambil 10 sampel pekarangan dan dicatat seluruh spesies tanaman yang terdapat pada pekarangan tersebut. Pemanfaatan tanaman ditentukan berdasarkan alasan penanaman dengan wawancara langsung dengan pemilik pekarangan. Analisis data meliputi kelimpahan, indek kekayaan, indek keanekaragaman dan indek kemerataan spesies tanaman. Hasil didapatkan sebanyak 64 famili yang terdiri dari 155 spesies dan 1265 individu. Famili yang banyak ditemukan jumlah spesiesnya adalah Araceae. Spesies yang memiliki kelimpahan tertinggi yaitu Garcinia mangostana dan Curcuma longa. Kelimpahan spesies tertinggi pada lahan pekarangan katagori luas. Keanekaragaman dan kekayaan spesies tanaman tertinggi pada lahan pekarangan kategori sempit, sedangkan kemerataan pada lahan kategori sedang. Habitus tanaman yang banyak dimanfaatkan adalah herba. Organ tanaman yang dimanfaatkan umumnya daun, sedangkan pemanfaatan tanaman pekarangan banyak digunakan sebagai sumber pangan. Kata kunci: Keanekaragaman; Garcinia mangostoma; herba; daun. ABSTRACTYard is a plot of land on which there are residential buildings and various plant species can be cultivated. This research aims to analyze the diversity and utilization of garden plants in Taripa Village, East Pamona District, Poso Regency, Central Sulawesi. Sampling was based on the area of the yard, namely the narrow (100-200 m2), medium (300-400 m2) and broad (500-600 m2). In each category, 10 samples of the yard were taken and recorded all plant species found in the yard. The use of plants is determined based on the reasons for planting by direct interviews with the owners of the yards. Data analysis includes abundance, Richness index, diversity index and index evenness of plant species. The results obtained were 64 families consisting of 155 species and 1265 individuals. The family with the most number of species found is Araceae. The species that had the highest abundance were Garcinia mangostana and Curcuma longa. The highest species abundance was in the large yard area. The highest diversity and richness of plant species was in the narrow category land, while evenness was in the medium category. Plant habitus that is widely used is herbaceous. The plant organs that are used are generally leaves, while the use of garden plants is widely used as a food source. Key words: Diversity; Garcinia mangostoma; herb; leaf.
DNA Isolation And Amplification of the rbcL (ribulose-1,5- bisphosphate carboxylase/oxygenase large subunit) gene of Caulerpa sp., Gracilaria sp., And Sargassum sp.
Authors: Tampanguma Biondi , Gerung Grevo S. , Warouw Veibe , Wagey Billy Th , Wulllur Stenly , Sumilat Deiske A. , Onibala Hens 
Published: April 2021

DNA isolation and gene amplification of algae are significantly influenced by various factors such as characteristics and components of the algae cell wall. Therefore techniques and methods of DNA isolation in certain algae, sometimes only succeed in one particular species and can not be applied to another algae species. Based on that issue, this study was conducted with the aims to determine the succeed of DNA isolation and amplify the rbcL gene as a target gene for identification. Algae DNA was isolated by using innuPrep Plant DNA commercial kit, and the second one with a modified conventional Cetyl Trimetyl Ammonium Bromide (CTAB) method, for the amplification process was using rbcL gene (ribulose-1,5-bisphosphate carboxylase / oxygenase large subunit) with two pairs of primers : rbcL 7F-753R and rbcL 577F-rbcSR. The results showed that the DNA of Gracilaria sp was succeed isolated by using CTAB method and it was denoted by the presence of DNA bands in agarose gel. Meanwhile DNA amplification for Gracilaria sp., and Sargassum sp., were succeed amplified with the appearance of DNA bands. But in algae Caulerpa sp., was only succeed on 1 pair of primary rbcL 7F and 7.Keywords : DNA, gene rbcL, algae Caulerpa sp., Sargassum sp., Gracilaria sp;AbstrakIsolasi DNA dan amplifikasi gen pada alga sangat dipengaruhi oleh beberapa faktor seperti karakter dan komponen pada dinding sel alga. Oleh karena itu proses isolasi DNA terkadang bisa berhasil pada satu jenis alga, namun tidak berhasil pada jenis alga lainnya. Oleh karena alasan tersebut, maka penelitian ini dilakukan untuk menentukan keberhasilan Isolasi DNA dan mengamplifikasi gen rbcL sebagai gen target identifikasi. Penelitian ini dilakukan dengan tahapan awal Isolasi DNA yang menggunakan kit komersil innuPrep Plant DNA Kit, dan metode konvensional Cetyl Trimetyl Ammonium Bromide (CTAB) yang telah dimodifikasi. Sedangkan untuk proses amplifikasi, menggunakan gen rbcL (ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit) digunakan dua pasang primer yaitu rbcL 7F-753R dan rbcL 577F-rbcSR. Hasil isolasi DNA dari alga Gracilaria sp berhasil diisolasi menggunakan metode CTAB yang ditandai dengan adanya pita DNA pada gel agarose. Amplifikasi DNA pada alga Gracilaria sp., dan Sargassum sp., berhasil diamplifikasi dengan munculnya pita DNA. Namun pada alga Caulerpa sp. hanya berhasil pada 1 pasang primer rbcL 7F dan753R.Kata kunci : DNA, gen rbcL, alga Caulerpa sp., Sargassum sp., Gracilaria sp.
Solar cell operation of sputter-deposited n-BaSi2/p-Si heterojunction diodes and characterization of defects by deep-level transient spectroscopy
Authors: Nemoto Taira , Aonuki Sho , Koitabashi Ryota , Yamashita Yudai , Mesuda Masami , Toko Kaoru , Suemasu Takashi 
Published: April 2021

Abstract
We form carbon-doped n-BaSi2(0.35 μm)/p-Si(111) heterojunction diodes by radio-frequency sputtering using BaSi2 and SiC targets, and demonstrate the solar cell operation for the first time under AM1.5 illumination. The electron trap level was measured to be 0.21 eV below the conduction band edge of the n-BaSi2 layers by deep-level transient spectroscopy. The shunt resistance reflecting the quality of the heterointerface exceeds 0.7 MΩ, one order of magnitude higher than that obtained for the same structure grown by molecular beam epitaxy. The carrier transport properties are discussed based on the external quantum efficiency spectrum and the band alignment.
<b>Espiritualidad maya y religión evangélica en Hacienda Vieja, en la segunda parte del siglo XX </b><br/><i>Maya spirituality and evangelical religion in Hacienda Vieja, in the second half of the twentieth century<i>
Authors: Esquit Edgar 
Published: April 2021

La historia de las comunidades mayas está estrechamente vinculada a la religiosidad que profesan sus miembros.A través de los años, la espiritualidad maya ha tenido una gran importancia en estas localidades pero, en la segunda parte del siglo XX, ha experimentado cambios importantes. El estudio que se presenta describe las transformaciones religiosas en una comunidad específica llamada Hacienda Vieja, en el municipio de San José Poaquil. Se narran los procesos que han protagonizado los habitantes de esta aldea cuando, en la década de los años sesenta del siglo XX, algunas familias se vincularon a la Iglesia Evangélica Centroamericana. En ese momento los aj q’ij (guías espirituales) fueron cuestionados pero, a pesar de ello, las nociones de la espiritualidad maya han mantenido su fuerza en la comunidad a lo largo del tiempo. Se analizan los procesos que ha vivido la comunidad en relación al pluralismo religioso y se concluye que la espiritualidad maya está abierta a la heterogeneidad de las experiencias religiosas que han nutrido a las aldeas a través de la historia. El estudio está basado en entrevistas a ancianos y gente adulta, se presentan como un relato histórico sobre la vida de una comunidad maya en Guatemala.
<b>Influencia de la situación social de la infancia guatemalteca para el desarrollo de la literatura infantil y juvenil nacional</b><br/><i>Influence of the social situation of Guatemalan children for the development of children’s and youth literatura<i>
Authors: Morales Barco Frieda Liliana 
Published: April 2021

Para entender al sujeto receptor de la literatura infantil y juvenil de Guatemala es necesario conocerlo. En ese sentido, este artículo presenta un enfoque cualitativo por medio de un método bibliográfico e historiográfico que permitió estudiar el proceso histórico de configuración de la noción de infancia en el país. Para el caso, se dividió en tres partes: (1) se describió la formación este concepto en Occidente y, luego, como se concibió en el país desde el período colonial hasta la actualidad; (2) se exploraron aspectos jurídicos y socio-históricos; (3) se reseñaron aspectos específicos de la literatura infantil y juvenil y sobre el acceso a la lectura y los libros. Los datos obtenidos apuntan, por un lado, hacia la comprensión de la infancia guatemalteca desde un punto de vistaproteccionista y no de formación ciudadana, lo que, por otro lado, evidencian los factores que influyen en el poco desarrollo del hábito y el gusto por la lectura. En ese sentido, es necesario seguir indagando sobre la infancia para que los especialistas, investigadores, mediadores, docentes, bibliotecarios puedan aproximarse a esta literatura con otra mirada.
- La guerra fantasma. Representaciones sobre el conflicto armado interno en San Pedro La Laguna durante los años 1981-1983
Authors: Cifuentes-Guerra Llomauri 
Published: April 2021

Cuando la violencia ejerce un papel importante en los asuntos humanos, al punto de llevarlos al campo de batalla, surgen imprevistos que, aun en las más calculadas circunstancias, dificultarán establecer certezas sobre el impacto de la destrucción. En San Pedro La Laguna, las condiciones que llevaron al ejército a monitorear la región, se asociaron con el ambiente cultural; es decir, la escolaridad de los pobladores, la cual era vasta y, por lo tanto, una amenaza. Sin embargo, para justificar la presencia del ejército, la comunidad no atestiguó actividades guerrilleras. A partir de esto surgen cuestionamientos sobre la concepción de “enemigo interno” que generó la población, las impresiones afectivas asociadas a la incertidumbre de quién podía ser calificado como “subversivo”, y las actitudes de los pobladores respecto al control ejercido por los grupos paramilitares en la locación. Para los fines, la metodología del estudio fue cualitativa puesto que se ha considerado, como base, el acervo documental del Organismo Judicial para identificar a las fuentes de interés. Y, además, fueron recuperadas las dimensiones representacionales del suceso histórico a través de la entrevista. Los resultados definen que los criterios para determinar al enemigo interno se percibían como arbitrarios, y esto producía estados de confusión, frustración e impotencia en la comunidad; sobre todo, debido a que estos criterios eran definidos por los grupos paramilitares, con el fin de asegurar intereses personales. Y, según los pobladores, las actuaciones represivas también eran utilizadas para saldar conflictos que, con frecuencia, eran surgidos de rencillas personales.
Percepciones y prácticas en torno a los síndromes culturales en comunidades q’eqchi’s
Authors: Paniagua Walter O. 
Published: April 2021

Este documento aborda las percepciones y prácticas en torno a los síndromes culturales que afectan a neonatos y lactantes dentro del grupo etnolingüístico q’eqchi’, específicamente en doce comunidades del distrito Campur del municipio de San Pedro Carchá, Alta Verapaz: Chelac, Secaj Raxquiché, Secum, Setzuj, San Vicente, Campur, Rubelchoc, Chiacam, Sejol, Bethania, Secum, Chamuchuj. La metodología empleada fue cualitativa y la recolección de datos se orientó a través del método etnográfico. Se realizaron 58 entrevistas (17 hombres y 41 mujeres) con diferentes actores comunitarios, entre ellos madres con hijos lactantes o en edad preescolar, jóvenes, padres de familia, comadronas, facilitadores comunitarios, vigilantes de salud y terapeutas indígenas. Los hallazgos de la investigación dan cuenta de la existencia de diferentes padecimientos como el Awas, Numay, Puub’ihix y Muh (sombra), que presentan elementos culturales en su diagnóstico y tratamiento. Estos padecimientos se generan al quebrantar normas establecidas dentro de las comunidades, por ello tienen un fuerte componente de control grupal en la búsqueda de alivio. De ahí que se evidencien diferentes itinerarios terapéuticos, dentro del proceso de sanación, que permiten explicar por qué las personas de estas comunidades prefieren los servicios prestados por los terapeutas indígenas y no el sistema estatal de salud. De igual forma, se presentan hallazgos relacionados con los roles de género que contribuyen en las demoras para la búsqueda de ayuda oportuna.