bluuebunny/crossref_metadata_2025_split huggingface dataset:
“Hyper-Hypermethylated Status” of the MGMT Gene Promoter Confers Additional Survival Benefits in IDH-Wild-Type Glioblastoma Patients
Authors: Kim Moonsik , Yoo Jihwan , Roh Yun Ho , Chang Jong Hee , Kim Se Hoon 
Published: February 2021

Abstract
IntroductionMethylation status of the O(6)-Methylguanine-DNA methyltransferase (MGMT) promoter plays key role in glioblastoma (GBM) with respect to the patient’s responses to temozolomide chemotherapy and disease prognosis. Although the cut-off value of MGMT methylation (≥10%) is currently widely used to dichotomize the MGMT status as “methylated” or “unmethylated” in pyrosequencing (PSQ) analysis, it is still unclear whether it reflects the actual MGMT methylation status of the patients. Thus, we investigated if there is a so called “hyper-hypermethylation cut-off value” that confers additional survival benefits in IDH-wild-type GBM patients. MethodsWe retrospectively analyzed a cohort of 110 isocitrate dehydrogenase (IDH)-wild-type GBM patients who underwent gross total resection followed by the standard treatment. Predictive “hyper-hypermethylated” cutoff values that yielded maximal differences in survival were investigated. ResultsThe estimated hyper-hypermethylated cutoff value was 40%. The median OS values for unmethylated, low-methylated, and hyper-hypermethylated groups were 16, 17, and 23 months, respectively. With regard to disease progression, the median progression-free survival (PFS) values were 11, 14, and 16 months, respectively. The hyper-hypermethylated MGMT cutoff values were correlated with significantly improved overall survival (OS), compared to the low-methylated (p=0.039, HR=3.86, CI=1.1 to 13.8) or unmethylated groups (p=0.023, HR=3.92, CI=1.21 to 12.6). ConclusionThus, in addition to the standard MGMT cut-off point of 10%, we suggest that a so-called “hyper-hypermethylated” MGMT cut-off point may confer additional survival benefits in IDH-wild-type GBM patients.
Preventing Nugget Shifting in Joining of Dissimilar Steels via Resistance Element Welding: A Numerical Simulation.
Authors: Rao Zhenghua , Liu Lei , Wang Yaqiong , Ou Liang , Liu Jiangwei 
Published: February 2021

Abstract
Joining the advanced high strength steels and the conventional steels is a critical issue for the manufacturing of lightweight vehicles. Resistance element welding (REW) is an emerging joining method for dissimilar metals and alloys by applying an auxiliary rivet-like resistance element in resistance spot welding (RSW). In this study, an electrical-thermal-mechanical coupled REW model for high-strength dual-phase (DP) steel and Q235 steel was developed by considering contact resistances as functions of temperature and surface contacting area. The results show that the welding element in REW serves to concentrate the current flow and thus Joule heat generation at the faying interface between the element and workpiece. For welding DP600 and Q235 workpieces with a small thickness ratio (≤0.4) or a high electrical resistivity ratio (≥3), REW could effectively mitigate nugget shifting between workpieces and reducing the thermal excursion to electrode as compared to RSW. Adding well-designed insulation layers in REW could further concentrate the current within the welding element, and enables a large-sized nugget at a lower current. This study is significant because it provides a better understanding to the electrical-thermal-mechanical behaviors with interfacial contacts in REW and contributes to its further advance.
Actirhodobacter Atrilutea Gen. nov., sp. nov., Isolated from the Surface Water of the Yellow Sea
Authors: Xue Hua-Peng , Zhang Dao-Feng , Xu Lin , Zhang Ai-Hua , Huang Jian-Ke , Liu Chuang 
Published: February 2021

Abstract
A gram-stain-negative, aerobic, orange-pigmented bacterial strain, designated as HHU K3-1T, was isolated from the surface water of the Yellow Sea. The strain was observed to grow on 2216E agar medium, and optimal growth occurred at pH 7.0, at 28 ℃ and with 2.0% (w/v) NaCl supplement. The major fatty acids (>10%) were summed feature 3 (C16:1ω6c/C16:1ω7c), C17:1ω6c and summed feature 8 (C18:1ω6c/C18:1ω7c). Strain HHU K3-1T was found to contain ubiquinone-10 as the predominant quinone and the major polar lipids were diphosphatidylglycerol (DPG), phosphatidylethanolamine (PE), phosphatidylglycerol (PG) and sphingoglycolipid (SGL). The 16S rRNA gene sequence analysis indicated that strain HHU K3-1T shared highest similarities with Pelagerythrobacter marensis KCTC 22370T (97.7%) and Qipengyuania oceanensis MCCC 1A09965T (96.9%). However, a phylogenetic tree based on 288 orthologous clusters (OCs) indicated that HHU K3-1T was close related to Parapontixanthobacter aurantiacus MCCC 1A09962T. The pairwise AAI and evolutionary distance between HHU K3-1T and Parapontixanthobacter aurantiacus MCCC 1A09962T is 67.1% and 0.43, respectively, which meet the recently proposed standard to differentiate genera in the family Erythrobacteraceae. On the basis of the result obtained by the polyphasic taxonomic study, the strain HHU K3-1T can be considered to represent a novel genus in the family Erythrobacteraceae, for which the name Actirhodobacter atrilutea gen. nov., sp. nov. is proposed. The type strain is HHU K3-1T (= MCCC 1K04225T = KCTC 72834T = CGMCC 1.17395T).
Temporal Changes in Immune Responses within the Tumor Microenvironment in the 4T1.2-HER2 Mammary Tumor Model
Authors: Xu Yitong , Rogers Connie 
Published: February 2021

Abstract
Background: The murine 4T1.2 triple-negative breast cancer model is widely used, but is poorly immunogenic with no defined tumor-associated antigens. A modified 4T1.2 model has been developed that stably expresses a surrogate tumor antigen, human epidermal growth factor receptor-2 (HER2). The goal of the current study was to characterize host immune responses in the 4T1.2-HER2 tumor model, focusing on the tumor microenvironment (TME) during the early stage of tumor development. Methods: Female BALB/c mice were orthotopically inoculated with 4T1.2-HER2 tumor cells and sacrificed at day (D) 6, 9, 12, 15 and 18 post tumor inoculation. The phenotype and function of tumor-infiltrating immune cells were assessed. Results: 4T1.2 and 4T1.2-HER2 tumor cells had similar proliferation rates in vitro. In contrast to the rapid progression of the parental 4T1.2 model, the 4T1.2-HER2 model demonstrated initial tumor growth followed by spontaneous tumor regression by D18 post tumor inoculation, which was not observed in scid mice. Following tumor regression, mice demonstrated either a second phase of tumor outgrowth or complete tumor rejection. Within the TME, the percentage of T cells was reduced at D9 and increased during tumor regression through D18 (p<0.05), whereas the percentage of myeloid-derived suppressor cells (MDSCs) increased during the initial tumor growth and was reduced by D18 (p<0.01). There was a stepwise increase in the percentage of IFNg+, IL-2+ and perforin+ T cells and NK cells peaking at D12-15. Furthermore, tumor regression occurred concurrently with HER2-specific IFNg production from tumor-infiltrating immune cells at D12 and D15 (p<0.05). During the second phase of 4T1.2-HER2 tumor growth, tumor volume was negatively correlated with immune infiltration (r=0.662, p=0.052). Conclusions: These results suggest that the integration of a surrogate tumor antigen, human HER2, into the clinically relevant, yet poorly immunogenic 4T1.2 breast cancer model enhanced its immunogenicity and induced HER2-specific immune responses.
Permeability Prediction in the South Georgia Rift Basin – Applications to CO2 Storage and Regional Tectonics
Authors: Akintunde Olusoga Martins , Knapp Camelia C. , Knapp James H. 
Published: February 2021

Abstract
The lack of the permeability log data necessary to assess reservoir injectivity as well as aid in the correlation and interpretation of existing porosity and resistivity logs for reservoir quality characterization for potential CO2 storage in the heterogenous and complex South Georgia Rift (SGR) basin provides the motivation for this study. The focus was on the Triassic-Jurassic red beds buried, entrenched beneath the Cretaceous-Cenozoic Coastal Plain sediments. Moreover, the significant cost typically between $10 M to $100M associated with drilling and logging for in situ permeability coupled with the limited resolution of existing core data further makes this work necessary. The purpose is to relate, use the interpretation of the predicted permeability distribution to assess feasibility for safe and long-term CO2 sequestration. This study also intends to establish the impacts of active and passive tectonism that has shaped and/or re-shaped the evolution of the basin on the present-day permeability. A methodology was applied that utilizes the pore space and geohydraulic properties of the reservoir from existing laboratory and well data to produce a newly derived permeability log. It shows a non-uniform distribution with depths possibly due to geologic changes in the confined and heterogeneous red beds. The derived log displays characteristics consistent with observations from the porosity and resistivity logs. The interpretation of these logs provides evidence for the presence of low permeable, tightly cemented and compacted red beds. We conclude that the low permeability aided by the low resistivity depicted in the red beds suggests increased confining stress and reduced injectivity, and that the uncharacteristically low permeability reflects a deformed basin shaped with episodes of uplift and erosion.
Vaporization Enthalpies and Vapor Pressures of the Major Components of Opopanax Oil, α-Santalene, cis α Bisabolene, cis α-Bergamotene,
Authors: Barton Dustin , Chickos James S 
Published: February 2021

Abstract
The vapor pressures and vaporization enthalpies of the major components of opopanax oil, a medicinal that has been in use since biblical times, are evaluated by correlation gas chromatography. Two sets of hydrocarbon standards are used, n-alkanes and a mixture of cyclic and polycyclic hydrocarbons. Two of the oil’s sesquiterpene components, evaluated in a previous study, were used both as standards and also as targets. Their use as targets was in an effort to confirm both their identity in the oil and the reproducibility of their properties. All correlations produce reproducible vaporization enthalpies and vapor pressures at ambient temperatures. At elevated temperatures, the use of the two different sets of standards resulted in some divergence in vapor pressure. Experiments are described aimed at attenuating this divergence. The results are compared to existing data.
Establishment of a Rodent Model of Neural Injury After High Frequency Monopolar Stimulation of the Motor Cortex
Authors: Yu Tao , Guo Lanjun , Russell Jennifer Sasaki , Sall Jeffrey W. 
Published: February 2021

Abstract
Direct electrical motor cortex stimulation with short-train high-frequency stimulation (HFS) for motor evoked potentials (MEPs) has been used intraoperatively during supratentorial surgeries, but the safety threshold is poorly defined. The goal of this study is to establish a rat model for the investigation of neural damage in the cerebral cortex caused by high current HFS to aid in defining safety thresholds. We performed bilateral craniotomy on 12 rats. Cerebral sensory-motor cortex was stimulated with a high-frequency current for 100 times. The rats were sacrificed and the brains were sliced for Nissl, DAPI, and IBA-1 staining. Severe neural damage of the cerebral cortex was found in all cases, including markedly shrunken and pyknotic cells. IBA-1 staining revealed reactive microglia morphology in the lesion area. DAPI staining showed nucleus degeneration and deformation. The cell density were significantly lower within the lesion area compared to the contralateral side. This study has established a brain lesion model caused by HFS on rats. These results suggest HFS may carry a risk of serious neural damage if repeatedly applied to the same brain site. More experiments are needed to fully understand the safety threshold of direct cortical stimulation with HFS for clinical use.
Time Series Analysis on Incidence of Pulmonary Tuberculosis With Weather Factors During 2004-2017 in Guangdong Province, China
Authors: Qin Tianyu , Hao Yu , He Juan 
Published: February 2021

Abstract
Background: Although the occurrence of some infectious diseases including TB was found to be associated with specific weather factors, few studies have incorporated weather factors into the model to predict the incidence of tuberculosis (TB). We aimed to establish an accurate forecasting model using TB data in Guangdong Province, incorporating local weather factors.Methods: Data of sixteen meteorological variables (2003-2016) and the TB incidence data (2004-2016) of Guangdong were collected. Seasonal autoregressive integrated moving average (SARIMA) model was constructed based on the data. SARIMA model with weather factors as explanatory variables (SARIMAX) was performed to fit and predict TB incidence in 2017. Results: Maximum temperature, maximum daily rainfall, minimum relative humidity, mean vapor pressure, extreme wind speed, maximum atmospheric pressure, mean atmospheric pressure and illumination duration were significantly associated with log(TB incidence). After fitting the SARIMAX model, maximum pressure at lag 6 (β= -0.007, P < 0.05, 95% confidence interval (CI): -0.011, -0.002, mean square error (MSE): 0.279) was negatively associated with log(TB incidence), while extreme wind speed at lag 5 (β=0.009, P < 0.05, 95% CI: 0.005, 0.013, MSE: 0.143) was positively associated. SARIMAX (1, 1, 1) (0, 1, 1)12 with extreme wind speed at lag 5 was the best predictive model with lower Akaike information criterion (AIC) and MSE. The predicted monthly TB incidence all fall within the confidence intervals using this model. Conclusions: Weather factors have different effects on TB incidence in Guangdong. Incorporating meteorological factors into the model increased the accuracy of prediction.
Clinical Outcomes in 2481 Unselected Real-World Patients Treated With a Polymer-Free Sirolimus-Eluting Stent: 3 Years Results From the NANO Multicenter Registry
Authors: Dai Yi , Wang Rutao , Chen Fengying , Zhang Yaojun , Liu Yi , Huang He , Yang Ping , Zhang Ruining , Zheng Bo , Gao Chao , Chen Yundai , Tao Ling 
Published: February 2021

Abstract
The long-term clinical data evaluating the safety and efficacy of the novel polymer-free sirolimus-eluting Nano plus stent (Lepu Medical, Beijing, China) is limited. We evaluated the 3-year clinical outcomes of a polymer-free sirolimus-eluting, Nano plus stent for the treatment of coronary artery disease in the NANO multicenter Registry. The NANO all-comers Registry trial was a prospective, multicenter clinical registry conducted in 26 centers between August 2016 and January 2017. A total of 2481 consecutive patients were exclusively treated with the Nano plus stent. The main clinical endpoint, target lesion failure (TLF, defined as cardiac death, target vessel nonfatal myocardial infarction, and clinically driven target lesion revascularization [CD-TLR]), was analyzed at 3 years. The incidence of TLF was 6.8% (168/2481). The rates of its individual components were as follows: cardiac death 3.8% (94/2481), target vessel nonfatal myocardial infarction 0.7% (18/2481), and CD-TLR 2.9% (68/2481). The rate of definite/probable stent thrombosis was 0.5% (13/2481). Diabetes mellitus, acute myocardial infarction, age, chronic renal failure, in-stent restenosis, chronic total occlusion and left ventricular ejection fraction <40% were the independent predictors of 3-year TLF. The TLF was relatively low in patients treated with polymer-free Nano plus stent at 3 years. The polymer-free Nano plus stent showed a favorable safety and efficacy profile in the real-world patients.Clinical trial registrationURL: https://www.clinicaltrials.gov/. Unique identifier: NCT02929030.
Approximate Stochastic Response of Hysteretic System With Fractional Element and Subjected to Combined Stochastic and Periodic Excitation
Authors: Kong Fan , Han Renjie , Zhang Yuanjin 
Published: February 2021

Abstract
A method based on statistical linearization is proposed, for determining response of the single-degree-of-freedom (SDOF) hysteretic system endowed with fractional derivatives and subjected to combined periodic and white/colored excitation. The method is developed by decomposing the system response into a combination of a periodic and of a zero-mean stochastic components. In this regard, first, the equation of motion is cast into two sets of coupled fractional-order non-linear differential equations with unknown deterministic and stochastic response components. Next, the harmonic balance method and the statistical linearization for the fractional-order deterministic and stochastic subsystems are used, to obtain the Fourier coefficients of the deterministic component and the variance of the stochastic component, respectively. This yields two sets of coupled non-linear algebraic equations which can be solved by appropriate standared numerical method. Pertinent numerical examples, including both softening and hardening Bouc-Wen hysteretic system endowed with different fractional-orders, are used to demonstrate the applicability and accuracy of the proposed method.
Synergistic Effects of Ca2+ and High-Valence Nb5+ co-Doping on the Structural, Optical and Magnetic Properties of BiFeO3
Authors: Xian Huimin , Tang Lingyun , Mao Zhongquan , Zhang Jiang , Chen Xi 
Published: February 2021

Abstract
The structural, optical and magnetic properties of BiFeO3, BiFe0.99Nb0.01O3 and Bi1 − xCaxFe0.99Nb0.01O3 (BCFNO, 0 ≤ x ≤ 0.25) nanoparticles synthesized via sol-gel method are investigated. It has been found that a phase transition from the rhombohedral R3c structure (x ≤ 0.10) to the ideal cubic perovskite structure (x = 0.25) which can be attributed to Ca2+ doping. Increasing Ca2+ dopants results in the increase of oxygen vacancies. As doping amount x increase, the band gap of BCFNO decreases and the valence band spectra indicates that it’s a p-type semiconductor, which indicates their favorable potential in photocatalytic applications. The remnant magnetization Mr of BCFNO reaches a maximum value (0.146 emu/g about 15 times compared with pure BFO) at x = 0.10. This enhancement of magneitic properties in BCFNO can be ascribed to the synergistic effect of A and B site ions co-doping. Higher valance Nb doping cause the size effect and the magnetic polarons bounded to the impurities by Ca ions.
Outcomes of Early-onset Preeclampsia With Severe Features at the University Hospital in Southern Thailand: a 15-year Experience
Authors: Khwankaew Noppasin , Sawaddisan Rapphon , Suwanrath Chitkasaem , Geater Alan 
Published: February 2021

Abstract
Purpose: To evaluate outcomes and factors associated with adverse outcomes among patients with early-onset preeclampsia with severe features at Songklanagarind Hospital. Methods: A retrospective study of 326 singleton women with early-onset preeclampsia with severe features treated at Songklanagarind Hospital between 2004-2019 was conducted. Baseline characteristics, management and outcomes were reviewed. Multivariate logistic regression was used to evaluate predictors of adverse outcomes. Statistical significance was set at p < 0.05.Results: There were no maternal mortalities, with 3.1% stillbirths and 6.7% neonatal deaths. High maternal serum creatinine (OR 3.26, 95% CI 1.27-8.36, p = 0.01) was significantly associated with adverse maternal outcomes. Early gestational age at delivery [< 28 weeks (OR 16.63, 95% CI 6.95-39.80, p <0.01), 28-32 weeks (OR 3.24, 95% CI 1.54-6.85, p <0.01)], maternal diabetes mellitus (OR 5.62, 95% CI 1.43-22.06, p = 0.01), high maternal serum creatinine (OR 2.66, 95%CI 1.20-5.93, p = 0.02) and elevated serum aminotransferases (OR 2.26, 95% CI 1.19-4.29, p = 0.01) were associated with serious adverse perinatal outcomes.Conclusions: Early-onset preeclampsia with severe features had favorable outcomes. Maternal diabetes mellitus, high serum creatinine, elevated serum aminotransferases and early gestational age at delivery were factors associated with poor outcomes.
Bereziskii-Kosterlitz-Thouless transition in the Weyl system PtBi2
Authors: Veyrat Arthur , Labracherie Valentin , Acharya Rohith , Bashlakov Dima , Caglieris Federico , Facio Jorge , Shipunov G. , Graf Lukas , Schoop Johannes , Naidyuk Yurii , Giraud Romain , Brink Jeroen van den , Buechner Bernd , Hess Christian , Aswartham Saicharan , Dufouleur Joseph 
Published: February 2021

Abstract
Symmetry breaking in topological matter became, in the last decade, a key concept in condensed matter physics to unveil novel electronic states. In this work, we reveal that broken inversion symmetry and strong spin-orbit coupling in trigonal PtBi2 lead to a Weyl semimetal band structure, with unusually robust two-dimensional superconductivity in thin fims. Transport measurements show that high-quality PtBi2 crystals are three-dimensional superconductors (Tc≈600 mK) with an isotropic critical field (Bc≈50 mT). Remarkably, we evidence in a rather thick flake (60 nm), exfoliated from a macroscopic crystal, the two-dimensional nature of the superconducting state, with a critical temperature Tc≈370 mK and highly-anisotropic critical fields. Our results reveal a Berezinskii-Kosterlitz-Thouless transition with TBKT≈310 mK and with a broadening of Tc due to inhomogenities in the sample. Due to the very long superconducting coherence length ξ in PtBi2, the vortex-antivortex pairing mechanism can be studied in unusually-thick samples (at least five times thicker than for any other two-dimensional superconductor), making PtBi2 an ideal platform to study low dimensional superconductivity in a topological semimetal.
Novel Hierarchical Structure of MoS2/TiO2/Ti3C2Tx&nbsp;Composites for Dramatically Enhanced Electromagnetic Absorbing Properties
Authors: Du Heng , Zhang Qipeng , Zhao Biao , Marken Frank , Gao Qiancheng , Lu Hongxia , Guan Li , Wang Hailong , Shao Gang , Xu Hongliang , Zhang Rui , Fan Bingbing 
Published: February 2021

Abstract
In order to prevent the microwave leakage and mutual interference, more and more microwave absorbing devices are added into the design of electronic products to ensure its routine operation. In this work, we have successfully prepared MoS2/TiO2/Ti3C2Tx hierarchical composites by one-pot hydrothermal method and focused on the relationship between structures and electromagnetic absorbing properties. Supported by comprehensive characterizations, MoS2 nanosheets were proved to be anchored on the surface and interlayer of Ti3C2Tx through a hydrothermal process. Additionally, TiO2 nanoparticles were obtained in situ. Due to these hierarchical structures, the MoS2/TiO2/Ti3C2Tx composites showed greatly enhanced microwave absorbing performance. The MoS2/TiO2/Ti3C2Tx composites exhibit a maximum reflection loss value of −33.5 dB at 10.24 GHz and the effective absorption bandwidth covers 3.1 GHz (13.9–17 GHz) at the thickness of 1.0 mm, implying the features of wide frequency and light weight. This work in the hierarchical structure MoS2/TiO2/Ti3C2Tx composites opens a promising door to the exploration of constructing extraordinary electromagnetic wave absorbents.