bluuebunny/crossref_metadata_2025_split huggingface dataset:
Biomolecular Electron Controller Composed of Nanobiohybrid with Electrically Released Complex for Spatiotemporal Control of Neuronal Differentiation
Authors: Lim Joungpyo , Yoon Jinho , Shin Minkyu , Lee Ki‐Bum , Choi Jeong‐Woo 
Published: December 2021

AbstractIn vitro spatiotemporal control of cell differentiation is a critical issue in several biomedical fields such as stem cell therapy and regenerative medicine, as it enables the generation of heterogeneous tissue structures similar to those of their native counterparts. However, the simultaneous control of both spatial and temporal cell differentiation poses important challenges, and therefore no previous studies have achieved this goal. Here, the authors develop a cell differentiation biomolecular electron controller (“Biomoletron”) composed of recombinant proteins, DNA, Au nanoparticles, peptides, and an electrically released complex with retinoic acid (RA) to spatiotemporally control SH‐SY5Y cell differentiation. RA is only released from the Biomoletron when the complex is electrically stimulated, thus demonstrating the temporal control of SH‐SY5Y cell differentiation. Furthermore, by introducing a patterned Au substrate that allows controlling the area where the Biomoletron is immobilized, spatiotemporal differentiation of the SH‐SY5Y cell is successfully achieved. Therefore, the proposed Biomoletron‐mediated differentiation method provides a promising strategy for spatiotemporal cell differentiation control with applications in regenerative medicine and cell therapy.
Recent Trends in Synthesis and Applications of Porous MXene Assemblies: A Topical Review
Authors: Chen Zhenyu , Asif Muhammad , Wang Ruochong , Li Yong , Zeng Xu , Yao Wentao , Sun Yimin , Liao Kin 
Published: December 2021

AbstractMXene possesses high conductivity, excellent hydrophilicity, rich surface chemistry, hence holds great potential in various applications. However, MXene materials have low surface area utilization due to the agglomeration of ultrathin nanosheets. Assembling 2D MXene nanosheets into 3D multi‐level architectures is an effective way to circumvent this issue. Incorporation of MXene with other nanomaterials during the assembly process could rationally tune and tailor the specific surface area, porosity and surface chemistry of the MXene assemblies. The complementary and synergistic effect between MXene and nanomaterials could expand their advantages and make up for their disadvantages, thus boost the performance of 3D porous MXene composites. Herein, we summarize the recent progress in fabrication of porous MXene architectures from 2D to 3D, and also discuss the potential applications of MXene nanostructures in energy harvesting systems, sensing, electromagnetic interference shielding, water purification and photocatalysis.
The Environmental Justice Dimension of the Mexican Emissions Trading System
Authors: Hernandez-Cortes Danae , Rosas-López Erick 
Published: December 2021

AbstractEmissions trading systems have the potential of increasing air quality given that GHG emissions are often co-produced with local pollutants such as NOx, SOx, and Particulate Matter (PM). Can emissions trading systems exacerbate or alleviate environmental justice concerns in emerging economies? According to the U.S. Environmental Protection Agency, Environmental Justice is achieved when no group is disproportionately affected by an environmental policy or phenomenon. The main objective of this chapter is to estimate the pollution burden faced by marginalized neighbourhoods in Mexico. This is relevant for Mexico given the beginning of the pilot program of the Mexican Emissions Trading System (ETS) and the country’s history of income inequality and poverty. Using linear regression and two-way fixed effects methods, we found that the highest emitters regulated under the ETS are located near poor populations. We estimated a 5$$\%$$
%
CO2 emissions-reduction scenario corresponding to national targets and associated NO2 emissions to that scenario. We find that this scenario is consistent with a decrease in the exposure of NO2 pollution for the most marginalized neighbourhoods. This chapter also discusses other potential sources of environmental injustice that could result after the beginning of the ETS and the potential to address them.
Bringing Emissions Trading Schemes into Mexican Climate Policy
Authors: Elizondo Alejandra 
Published: December 2021

AbstractEmissions trading schemes (ETS) have become popular as a policy instrument to tackle climate change. This chapter analyses the decision to deploy carbon markets and their interaction with other instruments in Mexico’s climate policy. Instrument selection has been thoroughly explored in the regulation and public policy literature (Kern et al. in Res Policy 48, 2019; Capano and Lippi in Policy Sci 50(2):269–293, 2016; Wurzel et al. in German Policy Studies 9:21–48, 2013; Harker et al. in Climate Policy 17(4):485–500, 2017; Baldwin et al. in Understanding regulation, Oxford University Press, 2012; Jordan et al. in Policy instruments in practice. Oxford handbooks online 536–549, 2011), but its application to carbon markets is mainly focused on environments such as Europe, the US and, more recently, China. The decision to adopt an ETS relies not only on specific characteristics of each instrument but also on institutional constraints and messy political considerations. A combination of preferences and institutional factors affect the choice of instruments, and the ultimate decision must be legitimate and instrumental for each context. I analyse the considerations involved in the deployment of the ETS pilot project, looking at its distinctive characteristics and those it shares with other available instruments, as well as the requirements for its implementation.
MicroRNA-193b impairs muscle growth in mouse models of type 2 diabetes by targeting the PDK1/Akt signalling pathway
Authors: Yang Shu , Yang Guangyan , Wu Han , Kang Lin , Xiang Jiaqing , Zheng Peilin , Qiu Shanhu , Liang Zhen , Lu Yan , Jia Lijing 
Published: December 2021

Abstract
Aims/hypothesis
Type 2 diabetes is associated with a reduction in skeletal muscle mass; however, how the progression of sarcopenia is induced and regulated remains largely unknown. We aimed to find out whether a specific microRNA (miR) may contribute to skeletal muscle atrophy in type 2 diabetes.

Methods
Adeno-associated virus (AAV)-mediated skeletal muscle miR-193b overexpression in C57BLKS/J mice, and skeletal muscle miR-193b deficiency in db/db mice were used to explore the function of miR-193b in muscle loss. In C57BL/6 J mice, tibialis anterior-specific deletion of 3-phosphoinositide-dependent protein kinase-1 (PDK1), mediated by in situ AAV injection, was used to confirm whether miR-193b regulates muscle growth through PDK1. Serum miR-193b levels were also analysed in healthy individuals (n = 20) and those with type 2 diabetes (n = 20), and correlations of miR-193b levels with HbA1c, fasting blood glucose (FBG), body composition, triacylglycerols and C-peptide were assessed.

Results
In this study, we found that serum miR-193b levels increased in individuals with type 2 diabetes and negatively correlated with muscle mass in these participants. Functional studies further showed that AAV-mediated overexpression of miR-193b induced muscle loss and dysfunction in healthy mice. In contrast, suppression of miR-193b attenuated muscle loss and dysfunction in db/db mice. Mechanistic analysis revealed that miR-193b could target Pdk1 expression to inactivate the Akt/mammalian target of rapamycin (mTOR)/p70S6 kinase (S6K) pathway, thereby inhibiting protein synthesis. Therefore, knockdown of PDK1 in healthy mice blocked miR-193b-induced inactivation of the Akt/mTOR/S6K pathway and impairment of muscle growth.

Conclusions/interpretation
Our results identified a previously unrecognised role of miR-193b in muscle function and mass that could be a potential therapeutic target for treating sarcopenia.

Graphical abstract
Glycation of N-ε-carboxymethyllysine
Authors: Hellwig Michael , Nitschke Julia , Henle Thomas 
Published: December 2021

AbstractThe Maillard reaction is traditionally subdivided into three stages that start consecutively and run in parallel. Here, we show that N-ε-carboxymethyllysine (CML), a compound formed in the late stage of the reaction, can undergo a second glycation event at its secondary amino group leading to a new class of Amadori rearrangement products. When N-α-hippuryl-CML was incubated in the presence of reducing sugars such as glucose, galactose, ribose, xylose, maltose, or lactose in solution for 1 h at 75 °C, the compound was degraded by 6–21%, and N-ε-carboxymethyl-N-ε-deoxyketosyl lysine derivatives were formed. Under the same conditions, lysine was 5–10 times more reactive than CML. N-α-hippuryl-N-ε-carboxymethyl-N-ε-(1-deoxyfructosyl)-l-lysine (hippuryl-CMFL) and N-ε-carboxymethyl-N-ε-(1-deoxyfructosyl)-l-lysine (CMFL) were synthesized, isolated and characterized by MS/MS and NMR experiments. Depending on the reaction conditions, up to 21% of CMFL can be converted to the furosine analogue N-ε-carboxymethyl-N-ε-furoylmethyl-l-lysine (CM-Fur) during standard acid protein hydrolysis with hydrochloric acid. Incubation of bovine serum albumin (BSA) with glucose for up to 9 weeks at 37 °C revealed the formation of CMFL in the protein as assessed by HPLC–MS/MS in the MRM mode. Under these conditions, ca. 13% of lysine residues had been converted to fructosyllysine, and 0.03% had been converted to CMFL. The detection of glycation products of glycated amino acids (heterogeneous multiple glycation) reveals a novel pathway in the Maillard reaction.
Completion surgery vs. primary TME for early rectal cancer: a national study
Authors: Lossius William J. , Stornes Tore , Myklebust Tor A. , Endreseth Birger H. , Wibe Arne 
Published: December 2021

AbstractPurposeWhile local excision by transanal endoscopic microsurgery (TEM) or transanal minimally invasive surgery (TAMIS) is an option for low-risk early rectal cancers, inaccuracies in preoperative staging may be revealed only upon histopathological evaluation of the resected specimen, demanding completion surgery (CS) by formal resection. The aim of this study was to evaluate the results of CS in a national cohort.MethodThis was a retrospective analysis of national registry data, identifying and comparing all Norwegian patients who, without prior radiochemotherapy, underwent local excision by TEM or TAMIS and subsequent CS, or a primary total mesorectal excision (pTME), for early rectal cancer during 2000–2017. Primary endpoints were 5-year overall and disease-free survival, 5-year local and distant recurrence, and the rate of R0 resection at completion surgery. The secondary endpoint was the rate of permanent stoma.ResultsForty-nine patients received CS, and 1098 underwent pTME. There was no difference in overall survival (OR 0.73, 95% CI 0.27–2.01), disease-free survival (OR 0.72, 95% CI 0.32–1.63), local recurrence (OR 1.08, 95% CI 0.14–8.27) or distant recurrence (OR 0.67, 95% CI 0.21–2.18). In the CS group, 53% had a permanent stoma vs. 32% in the pTME group (P = 0.002); however, the difference was not significant when adjusted for age, sex, and tumor level (OR 2.17, 0.95–5.02).ConclusionsOncological results were similar in the two groups. However, there may be an increased risk for a permanent stoma in the CS group.
A heuristic approach to the hyperparameters in training spiking neural networks using spike-timing-dependent plasticity
Authors: Połap Dawid , Woźniak Marcin , Hołubowski Waldemar , Damaševičius Robertas 
Published: December 2021

AbstractThe third type of neural network called spiking is developed due to a more accurate representation of neuronal activity in living organisms. Spiking neural networks have many different parameters that can be difficult to adjust manually to the current classification problem. The analysis and selection of coefficients’ values in the network can be analyzed as an optimization problem. A practical method for automatic selection of them can decrease the time needed to develop such a model. In this paper, we propose the use of a heuristic approach to analyze and select coefficients with the idea of collaborative working. The proposed idea is based on parallel analyzing of different coefficients and choosing the best of them or average ones. This type of optimization problem allows the selection of all variables, which can significantly affect the convergence of the accuracy. Our proposal was tested using network simulators and popular databases to indicate the possibilities of the described approach. Five different heuristic algorithms were tested and the best results were reached by Cuckoo Search Algorithm, Grasshopper Optimization Algorithm, and Polar Bears Algorithm.
Abundance and use pattern of wildcats of ancient human-modified cattle pastures in northern Iberian Peninsula
Authors: Jiménez-Albarral José J. , Urra Fermín , Jubete Fernando , Román Jacinto , Revilla Eloy , Palomares Francisco 
Published: December 2021

AbstractThe wildcat is typically found in low densities. Here, we estimated wildcat abundance in cattle pastures interspersed between forests in northern Iberian Peninsula, and their patterns of intra-annual and daily use. We censused wildcats three times daily (morning, afternoon, and night) from a car during 4 years in summer season. We also carried out four monthly tracks (two in the morning and two in the afternoon) for a complete year. Overall, we recorded 191 wildcats in pastures and 5 on the road in forest zones. Thirteen different individual wildcats were identified during the summer censuses, but only 29.9% of the wildcats observed (n = 196) could be assigned to an individual wildcat. The number of wildcats sighted decreased especially during the last year, when sightings were 52–67% lower than in previous years. Wildcats were seen significantly more during the morning and night censuses than during the afternoon ones. Estimated annual summer densities in pasture areas ranged between 0.11 and 0.46 sightings/km2. Throughout the year, wildcats were observed 5.9 times more during summer-autumn than during winter-spring, and they could be observed in any time of the day, but more often close to dusk. It is noteworthy that the ancient human-transformed landscapes for cattle pastures could be an important habitat for wildcats in northern Iberian Peninsula, and conserving these areas should be important to maintaining wildcat populations.
Stranded Assets: How Policy Uncertainty affects Capital, Growth, and the Environment
Authors: Bretschger Lucas , Soretz Susanne 
Published: December 2021

AbstractThe paper considers stochastic environmental policy and its effects on the environment, portfolio composition, and economic growth. Capital accumulation causes pollution which is reduced by private green services and public abatement. The government subsidizes green services and taxes dirty capital albeit at a rate which may become random, causing unexpected capital write-offs. Tax jumps depend on natural degradation and environmental activism. We derive how uncertainty and political activism affect the risk premia for investors. We analyze the incentives for firms to increase the greenness of production in order to reduce political uncertainty. Stochastic taxation is shown to act as a substitute for green subsidies when uncertainty decreases in the ratio of green services to capital and agents use their green activities strategically. Tax uncertainty may trigger precautionary savings, causing additional growth and enhanced environmental deterioration.
The production of contraceptive cyborgs in Swedish upper secondary sexuality education
Authors: Planting-Bergloo Sara , Orlander A. A. , Jakobson B. 
Published: December 2021

AbstractIn this study we examine upper secondary students’ notions of contraceptive methods, as human reproduction and contraception are common content in sexuality education in Sweden and worldwide. Our data were constructed during an extensive educational sequence in natural science sexuality education and include audio recordings of 17–18-year-old students’ stories. Since the main body of the stories was about hormonal and digital contraception and contraceptive responsibility, these stories are the focal point of our analysis. Our study further aims to problematize, challenge, and develop education on contraceptive methods, and Donna Haraway’s theoretical perspectives have been particularly useful. We have in the analytical process linked Haraway’s cyborg image with her later work on tentacular thinking. Our result shows that scientific facts about human reproduction are important for the students’ ability to navigate between the advantages and disadvantages of various contraceptive methods. However, sexuality education turns out to not only be a matter of scientific facts. This study accentuates how natural science, historical, political, cultural, and market-oriented intertwinings affect students’ notions of contraception—and thereby also the construction of natural science sexuality education.
Effects of Inter-Parental Conflict on Children’s Social Well-Being and the Mediation Role of Parenting Behavior
Authors: Hess Stephanie 
Published: December 2021

AbstractInter-parental conflicts can have devastating effects on children’s well-being and social behavior. This study explores the association between an increased frequency of inter-parental conflict and peer problems and prosocial behavior in children, and whether parents’ emotional warmth and negative communication with their children mediates this association. Data for a total of 1,157 children between the ages of 7 and 16 (4,016 observations) was drawn from the eighth to the twelfth waves of the German Family Panel so as to perform fixed-effects regressions and mediation analyses. The analyses reveal that increased inter-parental conflict is strongly related to decreased emotional warmth and increased negative communication in parents, and to increased peer problems and decreased prosocial behavior in children. The results also suggest that the association between inter-parental conflict and children’s social well-being is mediated by the parenting behavior of mothers and fathers, indicating that increased inter-parental conflict leads to less warm parenting and more negative communication in parents, which ultimately reduces children’s social well-being.
Correlation of Cerebral and Subcutaneous Glycerol in Severe Traumatic Brain Injury and Association with Tissue Damage
Authors: Hägglund Linda , Olivecrona Magnus , Koskinen Lars-Owe D. 
Published: December 2021

Abstract
Background
This study is a substudy of a prospective consecutive double-blinded randomized study on the effect of prostacyclin in severe traumatic brain injury (sTBI). The aims of the present study were to investigate whether there was a correlation between brain and subcutaneous glycerol levels and whether the ratio of interstitial glycerol in the brain and subcutaneous tissue (glycerolbrain/sc) was associated with tissue damage in the brain, measured by using the Rotterdam score, S-100B, neuron-specific enolase (NSE), the Injury Severity Score (ISS), the Acute Physiology and Chronic Health Evaluation Score (APACHE II), and trauma type. A potential association with clinical outcome was explored.

Methods
Patients with sTBI aged 15–70 years presenting with a Glasgow Coma Scale Score ≤ 8 were included. Brain and subcutaneous adipose tissue glycerol levels were measured through microdialysis in 48 patients, of whom 42 had complete data for analysis. Brain tissue damage was also evaluated by using the Rotterdam classification of brain computed tomography scans and the biochemical biomarkers S-100B and NSE.

Results
In 60% of the patients, a positive relationship in glycerolbrain/sc was observed. Patients with a positive correlation of glycerolbrain/sc had slightly higher brain glycerol levels compared with the group with a negative correlation. There was no significant association between the computed tomography Rotterdam score and glycerolbrain/sc. S-100B and NSE were associated with the profile of glycerolbrain/sc. Our results cannot be explained by the general severity of the trauma as measured by using the Injury Severity Score or Acute Physiology and Chronic Health Evaluation Score.

Conclusions
We have shown that peripheral glycerol may flux into the brain. This effect is associated with worse brain tissue damage. This flux complicates the interpretation of brain interstitial glycerol levels. We remind the clinicians that a damaged blood–brain barrier, as seen in sTBI, may alter the concentrations of various substances, including glycerol in the brain. Awareness of this is important in the interpretation of the data bedside as well in research.
Atomically Dispersed Transition Metal-Nitrogen-Carbon Bifunctional Oxygen Electrocatalysts for Zinc-Air Batteries: Recent Advances and Future Perspectives
Authors: Dong Fang , Wu Mingjie , Chen Zhangsen , Liu Xianhu , Zhang Gaixia , Qiao Jinli , Sun Shuhui 
Published: December 2021

AbstractRechargeable zinc-air batteries (ZABs) are currently receiving extensive attention because of their extremely high theoretical specific energy density, low manufacturing costs, and environmental friendliness. Exploring bifunctional catalysts with high activity and stability to overcome sluggish kinetics of oxygen reduction reaction and oxygen evolution reaction is critical for the development of rechargeable ZABs. Atomically dispersed metal-nitrogen-carbon (M-N-C) catalysts possessing prominent advantages of high metal atom utilization and electrocatalytic activity are promising candidates to promote oxygen electrocatalysis. In this work, general principles for designing atomically dispersed M-N-C are reviewed. Then, strategies aiming at enhancing the bifunctional catalytic activity and stability are presented. Finally, the challenges and perspectives of M-N-C bifunctional oxygen catalysts for ZABs are outlined. It is expected that this review will provide insights into the targeted optimization of atomically dispersed M-N-C catalysts in rechargeable ZABs.
Load-dependent Variable Gearing Mechanism of Muscle-like Soft Actuator
Authors: Wang Yingjie , Liu Chunbao , Ren Luquan , Ren Lei 
Published: December 2021

AbstractPennate muscle is characterized by muscle fibers that are oriented at a certain angle (pennation angle) relative to the muscle’s line of action and rotation during contraction. This fiber rotation amplifies the shortening velocity of muscle, to match loading conditions without any control system. This unique variable gearing mechanism, which characterized by Architecture Gear Ratio (AGR), is involves complex interaction among three key elements: muscle fibers, connective tissue, and the pennation angle. However, how three elements determine the AGR of muscle-like actuator is still unknown. This study introduces a Himisk actuator that arranges five contractile units at a certain pennation angle in a flexible matrix, the experiment and simulation results demonstrated that the proposed actuator could vary AGR automatically in response to variable loading conditions. Based on this actuator, we present a series of actuators by simulations with the varying pennation angle (P), elastic modulus of the flexible matrix (E), and number of contractile units (N) to analyze their effects on AGR, and their interaction by three-factor analysis of variance. The results demonstrated that P and N effect on the AGR significantly, while E effects on AGR slightly, which supported the idea that the P is the essential factor for the AGR, and N is also an important factor due to the capability of force generation. This provides a better understanding of mechanical behavior and an effective optimizing strategy to muscle-like soft actuator.