bluuebunny/crossref_metadata_2025_split huggingface dataset:
Abstract PO013: SNP rs1982 in the 3’UTR of placenta-specific protein 1 (PLAC1) is associated with endometrial cancer survival
Authors: Devor Eric J. , Gonzalez-Bosquet Jesus , Leslie Kimberly K. 
Published: February 2021

Abstract
Placenta-specific protein 1 (PLAC1) is a small (212aa) secreted protein normally only expressed on the apical surfaces of syncytiotrophoblasts. However, over the past two decades we and other investigators have shown that human cancers and, in particular, cancers of the human reproductive tract, induce PLAC1 expression. In gynecologic cancers, PLAC1 expression has been shown to be associated with risk, histology and TP53 mutation status. A previously unassessed informative SNP rs1982 (A&gt;C) lies in the 3’ untranslated region of the PLAC1 gene. We have begun to explore the utility of this SNP by genotyping a panel of 75 endometrial cancers. The panel is composed of 29 endometrial adenocarcinomas, 10 papillary serous adenocarcinomas, 9 mixed Mullerian tumors, 6 mixed histologies, 2 tumors identified as displaying complex atypia and 1 neuroendocrine tumor. High molecular weight genomic DNA was extracted from these tumors and SNP genotypes determined by directly sequencing a 312bp PCR amplicon generated by the primers:
rs1982for: GAGGATTGGTCTCTTCACACAG; rs1982rev: ACAATGTCTGGCCCATACTAAA
SNP genotypes were then evaluated against a variety of clinical variables including age, BMI, tobacco use, Charlson Index, co-morbidities, stage, grade, myometrial invasion, recurrence, and death by cancer using both univariate and multivariate logistic regression. Progression-free and overall survival were assessed by Cox Proportional Hazard ratio. Results show that CC homozygotes have increased progression-free and overall survival though neither reached statistical significance. However, several clinical variables were statistically associated with different rs1982 genotypes. Charlson co-morbidity Index was positively associated with presence of the wild-type A allele (OR = 1.61; 1.06 - 2.69; p&lt;0.05), while death from disease was negatively associated (OR = 0.15; 0.03 – 0.64; p&lt;0.02). These differences hold when accounting for other co-variates in the multivariate analysis. These data support further exploration of this SNP in gynecologic cancers.
Citation Format: Eric J. Devor, Jesus Gonzalez-Bosquet, Kimberly K. Leslie. SNP rs1982 in the 3’UTR of placenta-specific protein 1 (PLAC1) is associated with endometrial cancer survival [abstract]. In: Proceedings of the AACR Virtual Special Conference: Endometrial Cancer: New Biology Driving Research and Treatment; 2020 Nov 9-10. Philadelphia (PA): AACR; Clin Cancer Res 2021;27(3_Suppl):Abstract nr PO013.
Abstract PO032: Bacterial, archaea and viral transcripts (BAVT) expression in endometrial cancer
Authors: Nobre Silvana Pedra , Cardillo Nicholas , Bender David , Goodheart Michael , Devor Eric , Bosquet Jesus Gonzalez 
Published: February 2021

Abstract
Objectives: Microbes are found virtually everywhere, including the human body. High-throughput sequencing of microbial genome, also known as metagenomic sequencing, may help to understand interactions between human and microbial processes. In this pilot study we aimed to determine differences of bacterial, archaea and viral expression between normal fallopian tube samples and ovarian and endometrial cancer samples. Then, we assessed these differentially expressed transcripts in endometrial and ovarian cancer from TCGA data. Methods: RNA was extracted and sequenced from 62 endometrial cancers, 104 high-grade serous ovarian cancers and 12 normal tubes specimens from our Biobank repository. Sequencing was performed using the Illumina HiSeq4000 platform that sequences 150 bp paired-end transcripts. Reads were processed with Centrifuge, a novel algorithm that classifies metagenomic sequences. We built our own index to differentiate between bacterial, archaea, viral and human transcripts. Resulting number of transcripts were normalized, log transformed and compared between tubal epithelium and ovarian and endometrial cancers (t-test). Lasso regression was used to identify significant independent transcripts between samples. TCGA ovarian and endometrial samples were processed as previously, and expression of significant probes were evaluated. Results: Bacterial, archaea and viral transcripts (BAVT) were 4.7% and 3,1% of all transcriptome in tubal and ovarian cancer respectively. 266 transcripts were differentially expressed between normal tubes and ovarian and endometrial cancer, with a p&lt;0.005. 11 bacteria and 1 virus were independently significant in the multivariate regression model. These microbes were mapped close to genome regulatory areas. In general, there were more BVAT in normal tube samples. The amount of BAVT decreased in endometrial cancer and even more in ovarian cancer, supporting this regulatory role of BAVT. BAVTs in TCGA were 0.8% of all transcriptome. Conclusions: BAVT expression is different between normal tissues and cancers in the gynecologic tract. Cancer amount of BAVT seems to decrease in malignant tissues, supporting a regulatory role of BAVT. Further studies are needed to determine the significance of BAVT amount and change of expression.
Citation Format: Silvana Pedra Nobre, Nicholas Cardillo, David Bender, Michael Goodheart, Eric Devor, Jesus Gonzalez Bosquet. Bacterial, archaea and viral transcripts (BAVT) expression in endometrial cancer [abstract]. In: Proceedings of the AACR Virtual Special Conference: Endometrial Cancer: New Biology Driving Research and Treatment; 2020 Nov 9-10. Philadelphia (PA): AACR; Clin Cancer Res 2021;27(3_Suppl):Abstract nr PO032.
Mineralocorticoid Receptor in Smooth Muscle Contributes to Pressure Overload–Induced Heart Failure
Authors: Kim Seung Kyum , Biwer Lauren A. , Moss M. Elizabeth , Man Joshua J. , Aronovitz Mark J. , Martin Gregory L. , Carrillo-Salinas Francisco J. , Salvador Ane M. , Alcaide Pilar , Jaffe Iris Z. 
Published: February 2021

Background:
Mineralocorticoid receptor (MR) antagonists decrease heart failure (HF) hospitalization and mortality, but the mechanisms are unknown. Preclinical studies reveal that the benefits on cardiac remodeling and dysfunction are not completely explained by inhibition of MR in cardiomyocytes, fibroblasts, or endothelial cells. The role of MR in smooth muscle cells (SMCs) in HF has never been explored.

Methods:
Male mice with inducible deletion of MR from SMCs (SMC-MR-knockout) and their MR-intact littermates were exposed to HF induced by 27-gauge transverse aortic constriction versus sham surgery. HF phenotypes and mechanisms were measured 4 weeks later using cardiac ultrasound, intracardiac pressure measurements, exercise testing, histology, cardiac gene expression, and leukocyte flow cytometry.

Results:
Deletion of MR from SMC attenuated transverse aortic constriction-induced HF with statistically significant improvements in ejection fraction, cardiac stiffness, chamber dimensions, intracardiac pressure, pulmonary edema, and exercise capacity. Mechanistically, SMC-MR-knockout protected from adverse cardiac remodeling as evidenced by decreased cardiomyocyte hypertrophy and fetal gene expression, interstitial and perivascular fibrosis, and inflammatory and fibrotic gene expression. Exposure to pressure overload resulted in a statistically significant decline in cardiac capillary density and coronary flow reserve in MR-intact mice. These vascular parameters were improved in SMC-MR-knockout mice compared with MR-intact littermates exposed to transverse aortic constriction.

Conclusions:
These results provide a novel paradigm by which MR inhibition may be beneficial in HF by blocking MR in SMC, thereby improving cardiac blood supply in the setting of pressure overload–induced hypertrophy, which in turn mitigates the adverse cardiac remodeling that contributes to HF progression and symptoms.
Gestational Diabetes History and Glucose Tolerance After Pregnancy Associated With Coronary Artery Calcium in Women During Midlife
Authors: Gunderson Erica P. , Sun Baiyang , Catov Janet M. , Carnethon Mercedes , Lewis Cora E. , Allen Norrina B. , Sidney Stephen , Wellons Melissa , Rana Jamal S. , Hou Lifang , Carr John Jeffrey 
Published: February 2021

Background:
Gestational diabetes (GD) leads to earlier onset and heightened risk of type 2 diabetes, a strong risk factor for cardiovascular disease (CVD). However, it is unclear whether attaining normoglycemia can ameliorate the excess CVD risk associated with GD history. This study sought to evaluate GD history and glucose tolerance after pregnancy associated with coronary artery calcification (CAC) in women, a manifestation of atherosclerotic CVD and a predictor of CVD clinical events.

Methods:
Data were obtained from the CARDIA study (Coronary Artery Risk Development in Young Adults), a US multicenter, community-based prospective cohort of young Black (50%) and White adults aged 18 to 30 years at baseline (1985–1986). The sample included 1133 women without diabetes at baseline, who had ≥1 singleton births (n=2066) during follow-up, glucose tolerance testing at baseline and up to 5 times during 25 years (1986–2011), GD status, and CAC measurements obtained from 1 or more follow up examinations at years 15, 20, and 25 (2001–2011). CAC was measured by noncontrast cardiac computed tomography; dichotomized as Any CAC (score>0) or No CAC (score=0). Complementary log-log models for interval-censored data estimated adjusted hazard ratios of CAC and 95% confidence intervals for GD history and subsequent glucose tolerance groups (normoglycemia, prediabetes, or incident diabetes) on average 14.7 years after the last birth adjusted for prepregnancy and follow-up covariates.

Results:

Of 1133 women, 139 (12.3%) reported GD and were 47.6 years of age (4.8 SD) at follow-up. CAC was present in 25% (34/139) of women with GD and 15% (149/994) of women with no GD. In comparison with no GD/normoglycemia, adjusted hazard ratios (95% CIs) were 1.54 (1.06–2.24) for no GD/prediabetes and 2.17 (1.30–3.62) for no GD/incident diabetes, and 2.34 (1.34–4.09), 2.13 (1.09–4.17), and 2.02 (0.98–4.19) for GD/normoglycemia, GD/prediabetes, and GD/incident diabetes, respectively (overall
P
=0.003).

Conclusions:
Women without previous GD showed a graded increase in the risk of CAC associated with worsening glucose tolerance. Women with a history of GD had a 2-fold higher risk of CAC across all subsequent levels of glucose tolerance. Midlife atherosclerotic CVD risk among women with previous GD is not diminished by attaining normoglycemia.
Effects of Different High-Intensity Interval Training Regimens on Endurance and Neuroplasticity After Cerebral Ischemia
Authors: Pin-Barre Caroline , Hugues Nicolas , Constans Annabelle , Berton Eric , Pellegrino Christophe , Laurin Jérôme 
Published: February 2021

Background and Purpose:
The objective is to compare the effects of high-intensity interval training (HIIT) with long versus short intervals on endurance and motor performance. Their influence on neuroplasticity markers is assessed in the ipsilesional and contralesional cortex and hippocampus since their remodeling could improve functional recovery.

Methods:
Rats performed work-matched HIIT4 (long intervals: 4 minutes) or HIIT1 (short intervals: 1 minute) on treadmill for 2 weeks following transient middle cerebral artery occlusion. Forelimb grip strength evaluated motor function while incremental exercise tests measured the endurance performance. Key neuroplasticity markers were assessed by Western blot.

Results:
Both regimens were effective in enhancing both the speed associated with the lactate threshold and maximal speed at D8 and D15. Neuroplasticity markers were upregulated in the contralesional hemisphere after training contrary to the ipsilesional side. Grip strength completely recovered but is faster with HIIT4.

Conclusions:
HIIT with short and long intervals induced early aerobic fitness and grip strength improvements. Our findings revealed that neuroplasticity markers were upregulated in the contralesional cortex and hippocampus to promote functional recovery.
The Status of Franchising Laws in the Arab Region
Authors: Mattar Mohammed Y. 
Published: February 2021

Abstract
There has been a vast and rapid growth in the franchising business in the countries of the Middle East. The purpose of this article is to examine the status of franchising laws in the Arab World with particular focus on the commercial relationship between a franchisor and a franchisee. Most Arab countries rely on the traditional commercial agency laws to regulate the franchising business. These are designed to protect the interest of the national franchisee. Saudi Arabia is the first Arab country to adopt a comprehensive and independent law in the Arab region, while other countries rely on a code of conduct, an application of the distribution trade law or the implementation of the general rule of commercial agency. Saudi Arabia provides a detailed account of the franchising business, its establishment and its operation in an attempt to balance the interest of both, the franchisor and the franchisee.
Hurricane Predictability Analysis with Singular Vectors in the Multiresolution Global Shallow Water Model
Authors: Tian Xiaoxu , Ide Kayo 
Published: February 2021

AbstractIn this study, the tangent linear and adjoint (TL/AD) models for the Model for Prediction Across Scales (MPAS) Shallow Water (SW) component are tested and demonstrated. Necessary verification check procedures of TL/AD are included to ensure that the models generate correct results. The TL/AD models are applied to calculate the singular vectors (SVs) with a 48-h optimization time interval (OTI) under both the quasi-uniform-resolution (UR) and smoothly variable-resolution (VR) meshes in the cases of Hurricanes Sandy (2012) and Joaquin (2015). For the global domain, the VR mesh with 30 210 grid cells uses slightly fewer computational resources than the UR mesh with 40 962 cells. It is found that at the points before Hurricanes Sandy and Joaquin made sharp turns, the leading SV from the VR experiment show sensitivities in both areas surrounding the hurricane and those relatively far away, indicating the significant impacts from the environmental flows. The leading SVs from the UR experiments are sensitive to only areas near the storm. Forecasts by the nonlinear SW model demonstrate that in the VR experiment, Hurricane Sandy has a northwest turn similar to the case in the real world while the storm gradually disappeared in the UR experiment. In the case of Hurricane Joaquin, the nonlinear forecast with the VR mesh can generate a track similar to the best track, while the storm became falsely dissipated in the forecast with the UR mesh. These experiments demonstrate, in the context of SW dynamics with a single layer and no physics, the track forecasts in the cases of Hurricanes Sandy and Joaquin with the VR mesh are more realistic than the UR mesh. The SV analyses shed light on the key features that can have significant impacts on the forecast performances.
The Influence of South Pacific Convergence Zone Heating on the South Pacific Subtropical Anticyclone
Authors: Fahad Abdullah A. , Burls Natalie J. , Swenson Erik T. , Straus David M. 
Published: February 2021

AbstractSubtropical anticyclones and midlatitude storm tracks are key components of the large-scale atmospheric circulation. Focusing on the Southern Hemisphere, the seasonality of the three dominant subtropical anticyclones, situated over the South Pacific, South Atlantic, and south Indian Ocean basins, has a large influence on local weather and climate within South America, southern Africa, and Australia, respectively. Generally speaking, sea level pressure within the Southern Hemisphere subtropics reaches its seasonal maximum during the winter season when the Southern Hemisphere Hadley cell is at its strongest. One exception to this is the seasonal evolution of the South Pacific subtropical anticyclone. While winter maxima are seen in the South Atlantic and south Indian subtropical anticyclones, the South Pacific subtropical anticyclone reaches its seasonal maximum during local spring with elevated values extending into summer. In this study, we investigate the hypothesis that the strength of the austral summer South Pacific subtropical anticyclone is largely due to heating over the South Pacific convergence zone. Using added-cooling and added-heating atmospheric general circulation model experiments to artificially change the strength of austral summer diabatic heating over the South Pacific convergence zone, our results show that increased heating, through increased upper-level divergence, triggers a Rossby wave train that extends into the Southern Hemisphere midlatitudes. This propagating Rossby wave train creates a high and low sea level pressure pattern that projects onto the center of the South Pacific subtropical anticyclone to intensify its area and strength.
Roles of Tropical Remote Forcings on the South China Sea Winter Atmospheric and Cold Tongue Variabilities
Authors: Ce Seow Marvin Xiang , Morioka Yushi , Tozuka Tomoki 
Published: February 2021

AbstractInfluences from the tropical Pacific and Indian Oceans and atmospheric internal variability on the South China Sea (SCS) atmospheric circulation and cold tongue (CT) variabilities in boreal winter and the relative roles of remote forcings at interannual time scales are studied using observational data, reanalysis products, and coupled model experiments. In the observation, strong CT years are accompanied by local cyclonic wind anomalies, which are an equatorial Rossby wave response to enhanced convection over the warmer-than-normal western equatorial Pacific associated with La Niña. Also, the cyclonic wind anomalies are an atmospheric Kelvin wave response to diabatic cooling anomalies linked to both the decaying late fall negative Indian Ocean dipole (IOD) and winter atmospheric internal variability. Partially coupled experiments reveal that both the tropical Pacific air–sea coupling and atmospheric internal variability positively contribute to the coupled variability of the SCS CT, while the air–sea coupling over the tropical Indian Ocean weakens such variabilities. The northwest Pacific anticyclonic wind anomalies that usually precede El Niño–Southern Oscillation–independent negative IOD generated under the tropical Indian Ocean air–sea coupling undermine such variabilities.
The relationship between public service employees’ personal resources and psychological well-being
Authors: Kotzé Martina 
Published: February 2021

The study investigates the mediating role of psychological capital in the relationship between personal resources (mindfulness, self-leadership) and psychological well-being (burnout, work engagement) for a sample of 226 public sector employees in South Africa. Both mindfulness and self-leadership have a statistically significant positive influence on psychological capital, while psychological capital mediated the relationship between both mindfulness and work engagement, and mindfulness and burnout. psychological capital also mediates the relationship between both self-leadership and engagement, and self-leadership and burnout. Psychological capital has a statistically significant positive influence on work engagement and a statistically significant negative influence on burnout. The results show that mindfulness and self-leadership strategies can enhance psychological capital, reducing burnout and increasing engagement. Point for practitioners The public sector requires energised employees to serve the public despite demanding circumstances. Therefore, management must find ways to support employees in increasing work engagement levels and avoiding burnout. Since personal resources (mindfulness, self-leadership, psychological capital) are shown to enhance engagement and decrease burnout, managers and human resource managers can build these individual resources through training and development, and consider them in recruitment processes.
Parenting Style, Parental Personality, and Child Temperament in Children with Anxiety Disorders—A Clinical Study from India
Authors: Sahithya BR , Raman Vijaya 
Published: February 2021

Background: Anxiety disorders are common in children and contribute to adverse developmental outcomes. Although etiological models of child anxiety have identified various environmental factors, very few studies in India have examined these factors in children presenting with anxiety disorders. Therefore, the present study was designed to examine parenting styles, parental personality, and child temperament in children with anxiety disorders in an Indian outpatient setting. Methods: In total, 42 children with anxiety disorders and 42 typically developing children, matched on age and gender, were screened using Child Behavior Checklist, Color Progressive Matrices, and Screen for Child Anxiety Related Emotional Disorders Parent version. Their parents were screened using Mini International Neuropsychiatric Interview 5.0, following which they filled the questionnaires for parenting styles, parent personality, and child temperament. Results: There were significant differences between the two groups on parenting style, parent personality, and child temperament. Anxiety disorder was positively associated with the father’s permissiveness and negatively with the mother’s authoritativeness and child’s sociability. A combination of parenting styles and child temperament explained 69% of the variances in child anxiety disorders. There were significant associations between parental personality, child temperament, and parenting style. Parent and child characteristics explained 14%–46% of the variances in parenting styles. Conclusion: Results of this study are generally consistent with Western studies outlining the influence of child temperament and parenting styles on child outcome and have important implications for clinical management of anxiety disorders.
Utility of intraoperative magnetic resonance imaging for giant cell tumor of bone after denosumab treatment: a pilot study
Authors: Furuta Taisuke , Kubo Tadahiko , Sakuda Tomohiko , Saito Taiichi , Kurisu Kaoru , Muragaki Yoshihiro , Adachi Nobuo 
Published: February 2021

Background Giant cell tumor of bone (GCTB) is an intermediate but locally aggressive neoplasm. Current treatment of high-risk GCTB involves administration of denosumab, which inhibits bone destruction and promotes osteosclerosis. However, denosumab monotherapy is not a curative treatment for GCTB and surgical treatment remains required. Denosumab treatment complicates surgery, and the recurrence rate of GCTB is high (20%–30%). Purpose To examine the utility of intraoperative magnetic resonance imaging (iMRI) for detection and reduction of residual tumor after denosumab treatment and to investigate the utility of iMRI, which is not yet widely used. Material and Methods We enrolled five patients who received denosumab for a median period of eight months (range 6–12 months). Surgery was performed when the degree of osteosclerosis around the articular surface was deemed appropriate. We performed iMRI using a modified operation table to identify residual tumor after initial curettage and evaluated the rate of detection of residual tumor by iMRI, intraoperative and postoperative complications, exposure time of iMRI, and operation time. Results Suspected residual tumor tissue was identified in all five cases and was confirmed by histopathology after additional curettage. The rate of detection of residual tumor by iMRI was 100%. Residual tumor was located in sites which were difficult to remove due to osteosclerosis. The iMRI was performed safely and without trouble. During the median follow-up period of 10 months (range 6–24 months), no adverse events or recurrences occurred. Conclusion Intraoperative MRI could contribute to the reduction of residual tumor tissue and it may prevent recurrence of GCTB after denosumab therapy.