bluuebunny/crossref_metadata_2025_split huggingface dataset:
Copy-Number Variants in The Contactin-5 Gene Are a Potential Risk Factor for Autism Spectrum Disorder
Authors: Schmilovich Zoe , Huguet Guillaume , He Qin , Musa-Johnson Amélie , Douard Elise , Loum Mor Absa , Liao Calwing , Ross Jay P. , Dionne-Laporte Alexandre , Spiegelman Dan , Hayward Caroline , Banaschewski Tobias , Bokde Arun , Desrivieres Sylvane , Lemaitre Herve , Schumann Gunter , Xiong Lan , Dion Patrick A. , Jacquemont Sébastien , Chaumette Boris , Rouleau Guy A. 
Published: July 2021

Abstract

BackgroundContactin-5 (CNTN5) is a candidate risk gene for autism spectrum disorder (ASD), yet previous attempts to associate copy-number variants (CNVs) encompassing CNTN5 with ASD-susceptibility were limited by insufficient statistical power. Here, we aim to clarify the putative association between CNTN5 CNVs and ASD-risk using large samples. MethodsFirst, we calculated the prevalence and transmission of CNTN5 CNVs in ASD across three ASD cohorts (SSC, MSSNG, and SPARK), the cases reported in the Mercati et al. study, and the BBGRE database (n = 16,607). Second, we modelled their transmission in children with ASD compared to their unaffected siblings. Third, we assessed their frequency in cases with ASD compared to unselected population controls (n = 24,898) and replicated the findings in UK Biobank (UKBB), an independent general population cohort (n = 459,855). Finally, we evaluated the clinical impact of CNTN5 CNVs by assessing their enrichment in a broad neurodevelopmental disorder (NDD) cohort, and the clinical profile of CNTN5 CNV carriers in the DECIPHER database.ResultsThe prevalence of CNTN5 exonic deletions and duplications was stable across ASD and across unselected cohorts (0.042% and 0.020%, respectively). We found a significant enrichment of intronic CNTN5 deletions CNVs in ASD compared to unselected controls (0.175% and 0.004%, respectively). CNVs in most cases with ASD (29 out of 30, 96.7%) were inherited. Parents transmitted the variants to their affected and unaffected children with the same frequency. No differences in exonic CNTN5 CNVs enrichment between cases with ASD compared to individuals with NDDs was observed. LimitationsThe lack of phenotypic data available for unaffected family members of probands with ASD limits the potential to assess whether CNTN5 CNVs segregate with other neuropsychiatric or sub-threshold autistic traits. Different genotyping or sequencing technologies may affect the differences in CNTN5 CNV prevalence across cohorts.ConclusionCNTN5 CNVs are rare inherited ASD susceptibility variants. They may also confer risk for other neuropsychiatric disorders. We offer a powerful framework to investigate candidate susceptibility variants that may not be detected through small-scale approaches. This approach may reveal more intermediate effect-size variants that are implicated in the etiology of ASD.
Genome-based taxonomic reclassification of Acinetobacter species using type and reference strains
Authors: Suzuki Masato , Maehana Shotaro , Kitasato Hidero 
Published: July 2021

Abstract
Acinetobacter species are widely distributed in the environment and clinical settings worldwide and serve as natural reservoirs of antimicrobial resistance genes and occasional human pathogens responsible for nosocomial infections. In this study, we performed genomic analysis of Acinetobacter seohaensis DSM 16313, a type strain of the proposed Acinetobacter species. This species was estimated to be evolutionary close to Acinetobacter towneri but the genome sequence of A. seohaensis was not publicly available. Pangenome analysis of the genome sequence of A. seohaensis along with those of genome-available type and reference strains of 82 Acinetobacter species including A. towneri suggested that three groups of Acinetobacter species, A. seohaensis and A. towneri; Acinetobacter pullorum and Acinetobacter portensis; and Acinetobacter idrijaensis, Acinetobacter mesopotamicus, and Acinetobacter lwoffii, were phylogenetically very similar to each other. Genome comparisons based on in silico DNA-DNA hybridization and the average nucleotide identity confirmed that these three groups of Acinetobacter species are conspecific. Based on the rules of priority, A. seohaensis, A. pullorum, and A. idrijaensis/A. mesopotamicus should be reclassified as later heterotypic synonyms of A. towneri, A. portensis, and A. lwoffii, respectively.
Complete Z-intuitionistic Fuzzy MULTIMOORA Method with AHP and its Application to COVID-19
Authors: Zhang Yijin , Huang Jie , Lin Zongbing 
Published: July 2021

Abstract
For emergencies, the reliability of information can not be guaranteed. At the same time, due to the lack of information and knowledge, neither the criteria itself nor the credibility can be given a precise evaluation by decision-makers(DMs). Therefore, we combine intuitionistic fuzzy set and Z-number to get a new class of fuzzy set, complete Z-intuitionistic fuzzy set(CZIFS), and its degenerate form, A-type Z-intuitionistic fuzzy set(AZIFS) and B-type Z-intuitionistic fuzzy set(BZIFS). CZIFS can serve as a reliable tool to depict the hesitant degree both on the ambiguity and reliability of uncertain information. In addition, we introduce the score and accuracy functions and distance measure of complete Z-intuitionistic fuzzy number(CZIFN), with which we have considered both reliability information and DMs' preference on it. Then, we improve traditional MULTIMOORA by developing reference point(RP) model to consider both the risk and profile of alternatives and integrating analytic hierarchy process(AHP) in the process of ranking aggregation method to take into account the preference of DMs on three subordinate rankings. Besides, to solve multicriteria group decision making(MCGDM) problem, we develop improved MULTIMOORA method to the environment of CZIFN. Finally, to illustrate the proposed method, we give a numerical example, solving site selecting of Fangcang shelter hospital for COVID-19.
Pandemic Preparedness of Countries to Cope With COVID-19
Authors: Coccia Mario 
Published: July 2021

Abstract
One of the problems hardly clarified in COVID-19 pandemic crisis is the effective of the pandemic preparedness of countries to cope with COVID-19 and reduce negative effects in society. The study here confronts this problem by proponing the Index of resilience that detects which countries have had the best performance to reduce the negative impact of mortality related to COVID-19 pandemic and the Index of preparedness that assesses performance of countries to support COVID-19 vaccinations and prevent future waves of COVID-19. The application of these indexes on selected European countries suggests that, in average, best-performer countries to cope with COVID-19 pandemic crisis have a smaller size of population and high level of health expenditures. However, lessons learned from this study are that manifold countries have several biological security weaknesses and low pandemic preparedness. The policy implications are that governments should revise and reinforce, planning, institutions and overall organization devoted to face pandemic threats.
Combined use of LS-PIV and CFD for the characterization of turbulent flow in the contact chamber of ‘COSTA AZUL’ wastewater treatment plant, Carlos Paz
Authors: Matias Ragessi I. , Gianina Leyria Rainieri , Facundo Ganancias , Marcelo García C. , Melina Di Buo , Gerardo Balangero Bottazzi 
Published: July 2021

Abstract
The Costa Azul wastewater treatment plant is situated in Carlos Paz city (Córdoba, Argentina). The treated water is discharged to the San Roque reservoir, which is the source of water for the city of Córdoba and surrounding towns. Consequently, it is of great importance to evaluate the potential impact on the water quality reservoir for the preservation of public health. Currently, the WWTP lacks an accurate flow monitoring system at the last treatment: disinfection. This problem led to the development of a methodology in which experimental in-situ work and computational fluid dynamics (CFD) were applied, in a combined way, to calibrate the flow measurement system. First, large-scale particle image velocimetry (LS-PIV) was applied. The results made it possible to obtain characteristic parameters of the average flow: cross-sectional velocity profiles, recirculation and flow stagnation zones, flow discharge and characteristic parameters of the contact chamber. Second, numerical models, based on the Reynolds-average Navier–Stokes equations with the k–ε turbulent closure model were used. Based on the simulation results, it was possible to calibrate the discharge equation for a rectangular weir, and elaborate recommendations to improve the discharge measurement system and hydraulics in the area of the contact chamber curves.
Comprehensive evaluation of water resources security of the Huaihe Eco-economic Belt
Authors: Ruan Jun , He Gang 
Published: July 2021

Abstract
The study of water resource security is a basic scientific issue that must be faced in the construction of the ecological environment. To examine the status of regional water resources security in the Huaihe Eco-economic Belt, this study builds a comprehensive evaluation index system based on the ‘Driving-Pressure-State-Impact-Response’ (DPSIR) framework, combines the entropy weight method and Lotka–Volterra symbiosis model to calculate the water resources security status from 2010 to 2018. Then the fixed effects regression model is used to analyze the factors affecting the water resources system. The results show that: (1) The status of water resources security in the Huaihe Eco-economic belt decreased from 2011 to 2017 and, thereby transitioned from a safe to a dangerous state. The coordination index of the economic system and water resource system was only −0.17 in 2017, and the partial development model benefited the economy but damaged the water resources. (2) The security status of 25 prefecture-level cities improved significantly, and the number of cities with status of alert or above increased from 11 to 15. However, there are apparent differences among the regions. The status has shifted to ‘north and south being better than east and west’. The water resources security status of Jining, Linyi, and Lu'an have improved, whereas Yancheng, Taizhou, Pingdingshan, and other cities showed rather poor development during 2017. (3) The correlation coefficient of the economic system was −0.154 and hindered the development of the water resources system. The correlation coefficients of the added value of the tertiary industry and the expenditure on energy conservation and environmental protection was 0.699 and 0.180 respectively and played a positive role in promoting the water resources system. It is necessary to optimize and adjust the industrial structure and protect the environment.
Mechanistic Aspects of Medicinal Plants and Secondary Metabolites against Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2)
Authors: Malekmohammad Khojasteh , Rafieian-Kopaei Mahmoud 
Published: July 2021

Background and Objective:
Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), a
highly pathogenic virus, is responsible for a respiratory disease termed coronavirus disease 2019 (COVID-19).
SARS-CoV-2 genome encodes various structural and non-structural proteins, which are necessary for viral entry
and replication. Among these proteins, papain-like protease (PLpro), 3C-like protease (3CLpro), RNAdependent
RNA polymerase (RdRp), helicase, serine protease, and spike protein are potential targets of herbal
remedies and phytocompounds for inhibition of viral infection and replication. There is, at present, no confirmed
cure for the COVID-19. Various plants and their components have been introduced against SARS-CoV-2.
A number of review articles have also been published on them. This article is focused on the mechanistic aspects
of these plants and their derivatives on SARS-CoV-2.

Methods:
The material in this review article was prepared from significant scientific databases, including Web
of Science, PubMed, Science Direct, Scopus and Google Scholar.

Results:
Different medicinal plants and their phytocompounds interact with important structural and nonstructural
of SARS-CoV-2 proteins. Natural compounds form strong bonds with the active site of SARS-CoV-2
protease and make large conformational changes. These phytochemicals are potential inhibitors of structural
and non-structural SARS-CoV-2 proteins such as Spike protein, PLpro, and 3CLpro. Some important anti-
SARS-CoV-2 actions of medicinal plants and their metabolites are: inhibition of the virus replication or entry,
blocking the angiotensin-converting enzyme 2 (ACE-2) receptor and “Transmembrane protease, serine 2
(TMPRSS2), regulation of inflammatory mediators, inhibition of endothelial activation, toll-like receptors
(TLRs) and activation of the nuclear factor erythroid-derived 2-related factor 2 (Nrf2). Some of these important
natural immune boosters that are helpful for the prevention and cure of various symptoms related to COVID-19
include Allium sativum, Nigella sativa, Glycyrrhiza glabra Zingiber officinalis, Ocimum sanctum, Withania
somnifera, Tinospora cordifolia, and Scutellaria baicalensis. Also, Kaempferol, Quercetin, Baicalin, Scutellarin,
Glycyrrhizin, Curcumin, Apigenin, Ursolic acid, and Chloroquine are the best candidates for treating the
symptoms associated with SARS-CoV-2 infection.

Conclusion:
Medicinal plants and/or their bioactive compounds with inhibitory effects against SARS-CoV-2 support
the human immune system and help in fighting against COVID-19 and rejuvenating the immune system.
Recombinant Active Peptides and their Therapeutic Functions
Authors: Ajingi Ya’u Sabo , Rukying Neeranuch , Aroonsri Aiyada , Jongruja Nujarin 
Published: July 2021

Abstract:Recombinant active peptides are utilized as diagnostic and biotherapeutics in various maladies and as bacterial growth inhibitors in the food industry. This consequently stimulated the need for recombinant peptides' production, which resulted in about 19 approved biotech peptides of 1-100 amino acids commercially available. While most peptides have been produced by chemical synthesis, the production of lengthy and complicated peptides comprising natural amino acids has been problematic with low quantity. Recombinant peptide production has become very vital, cost-effective, simple, environmentally friendly with satisfactory yields. Several reviews have focused on discussing expression systems, advantages, disadvantages, and alternatives strategies. Additionally, the information on the antimicrobial activities and other functions of multiple recombinant peptides is challenging to access and is scattered in literature apart from the food and drug administration (FDA) approved ones. From the reports that come to our knowledge, there is no existing review that offers substantial information on recombinant active peptides developed by researchers and their functions. This review provides an overview of some successfully produced recombinant active peptides of ≤100 amino acids by focusing on their antibacterial, antifungal, antiviral, anticancer, antioxidant, antimalarial, and immune-modulatory functions. It also elucidates their modes of expression that could be adopted and applied in future investigations. We expect that the knowledge available in this review would help researchers involved in recombinant active peptide development for therapeutic uses and other applications.
Potential Inhibitor of Adenylyl Sulfate Reductase Isolated from Desulfovibrio
desulfuricans with PU/PU-Ag to Control Pitting Corrosion of Oil
Tanks and Pipelines
Authors: Koush Wafaa A. , Labena Ahmed , Elsawy Hany , Farahat Laila A. , Mohamed Tarek M. , Salem Maha M. 
Published: July 2021

Background and Aim:
This study aims to alleviate the microbiologically affected corrosion
that occurred by sulfate-reducing bacteria (SRB) through synthesizing a bio-based polyurethane
polymer and its nanocomposite coating, silver nanoparticles (PU-Ag). Moreover, it will also
evaluate the effect of PU alone and PU-Ag as inhibitors for adenylyl sulfate reductase (APS), the
main enzyme for sulfate reduction.

Methods:
In this study, the PU was prepared from vegetable soybean oil, and the silver nanoparticles
(Ag-NPs) with a concentration of 1% were coated to the PU forming a nanocomposite. The
PU and the PU-Ag were characterized and evaluated as inhibitors of the APS reductase enzyme.

Results:
The results obtained from FTIR, UV, DLS, TEM, and XRD confirmed the preparation
structure of the PU and PU-Ag. Furthermore, the PU/PU-Ag competitively inhibited the APS reductase
with an inhibition constant equal to 35.7 and 11 mg, respectively. These indicated the exerted
inhibitory effect of PU/PU-Ag upon the activity of the APS reductase enzyme.

Conclusion:
The APS reductase enzyme produced by SRB, which is recorded as a big problem in
the oil and gas industry such as pitting corrosion of tanks and pipelines, could be inhibited by PU
and PU-Ag.
KONTRIBUSI PENGALAMAN PRAKERIN TERHADAP MINAT BERWIRAUSAHA SISWA SMK PROGRAM STUDI KEAHLIAN TEKNIK BANGUNAN
Authors: Hasbi Hasbi 
Published: July 2021

ABSTRACTEntrepreneurship education in order to foster interest in entrepreneurship for vocational high school students is an important aspect in accustoming graduates who are able to create employment. Sample of this research was entire Vocational High School students of Architecture Engineering Courses in Malang who has carried out on-the job training as many as 108 students. Based on the analysis by looking at propensity scores showed that: students’ entrepreneurship categorized as high contribution. From 108 respondents, 11 students or 10.26% categorized as very high contribution, 47 students or 43.52%, categorized as high contribution, 28 students or 25.92%, categorized as adequate contribution, and 22 students or 20.38% categorized as low contribution. Based on these results, it can be concluded that interest in entrepreneurship is influenced by experience of on-the-job-training which experience provides knowledge and experience, so as to accustom a student's interest in entrepreneurship. Keywords: entrepreneurship interest, On-the-Job Training experience, vocational high school ABSTRAKPenanaman kewirausahaan dalam menumbuhkan minat berwirausaha bagi siswa SMK merupakan aspek penting dalam menghasilkan lulusan yang mampu menciptakan lapangan kerja (berwirausaha). Sampel dalam penelitian ini adalah seluruh siswa SMK Paket Keahlian Teknik Gambar Bangunan di Malang yang telah melaksanakan Prakerin sebanyak 108 orang siswa. Berdasarkan hasil analisis dengan melihat kecenderungan skor yang diperoleh, minat berwirausaha siswa termasuk dalam kategori tinggi. Dari 108 orang responden, yang termasuk pada kategori sangat tinggi sebanyak 11 orang atau sekitar 10,26%;, kategori tinggi sebanyak 47 orang atau sekitar 43,52%, kategori cukup sebanyak 28 orang atau sekitar 25,92%, dan kategori kurang sebanyak 22 orang atau sekitar 20,38%. Berdasarkan hasil penelitian tersebut, maka dapat disimpulkan bahwa Minat berwirausaha dipengaruhi oleh pengalaman prakerin dimana pengalaman memberikan pengetahuan dan pengalaman dalam bekerja, sehingga dapat membentuk minat siswa dalam berwirausaha. Kata kunci: minat berwirausaha, pengalaman prakerin, sekolah menengah kejuruan
PENGARUH INTERAKSI SOSIAL GURU DENGAN SISWA TERHADAP MOTIVASI BELAJAR DI JURUSAN TEKNIK GAMBAR BANGUNAN SMK NEGERI 1 CILAKU CIANJUR
Authors: Iswardhany Rieske , Rahayu Sri 
Published: July 2021

Interaksi sosial guru dengan siswa sebagai faktor eksternal yang mampu mempengaruhi motivasi belajar siswa. Dalam proses belajar mengajar perlu sekali adanya interaksi guru dengan siswa yang komunikatif, kondisi yang menyenangkan dan suasana keakraban antara guru dan siswa. Sehingga dengan adanya rasa senang kepada guru. Maka, siswa dan siswi akan termotivasi untuk belajar. Penelitian ini bertujuan untuk 1) memperoleh gambaran Interaksi Sosial guru dengan siswa di Jurusan TGB SMK Negeri 1 Cilaku Cianjur; 2) Memperoleh gambaran Motivasi siswa di Jurusan TGB SMK Negeri 1 Cilaku Cianjur; 3) Mengetahui seberapa besar Pengaruh pengaruh Interaksi Sosial guru dengan siswa terhadap motivasi belajar jurusan TGB SMK Negeri 1 Cilaku Cianjur. Penelitian ini merupakan penelitan Deskriptif dengan menggunakan pendekatan kuantitatif. Instrumen pengumpulan data dengan menggunakan angket. Sampel penelitian dalam penelitian ini berjumlah 66 siswa dari jumlah populasi 66 siswa kelas XI program keahlian TGB SMK Negeri 1 Cilaku Cianjur. Dari hasil analisis data diperoleh hubungan yang terjadi antara kedua variabel tersebut positif dan signifikan. Gambaran interaksi sosial siswa dengan guru dalam kriteria baik. Sedangkan gambaran umum dari motivasi belajar siswa di jurusan TGB SMK Negeri 1 Cilaku Cianjur adalah tinggi. Berdasarkan pada kriteria penafsiran koefisien korelasi, hubungan antara kedua variabel tersebut termasuk dalam kategori kuat.
EVALUASI PENERAPAN KESELAMATAN DAN KESEHATAN KERJA (K3) PADA PRAKTIK FINISHING BANGUNAN SMK NEGERI 2 YOGYAKARTA
Authors: Erfian Monica , Raharjo Nuryadin Eko 
Published: July 2021

Penelitian ini bertujuan untuk mengetahui: (1) antecedents (input), transcription (process), dan output (hasil) dari penerapan Keselamatan dan Kesehatan Kerja (K3) di SMK Negeri 2 Yogyakarta pada praktik finishing bangunan, (2) pemahaman siswa terhadap penerapan K3 di bengkel Kompetensi Keahlian Konstruksi Gedung Sanitasi dan Perawatan (KGSP). Metode evaluasi yang digunakan adalah Countenance Stake dengan 3 tahapan meliputi antecedents (input), transcription (process), dan output. Subyek penelitian adalah siswa kelas XI KGSP SMK Negeri 2 Yogyakarta. Obyek penelitian adalah penerapan Keselamatan dan Kesehatan Kerja (K3). Data dikumpulkan melalui wawancara, pengamatan, dan dokumentasi. Data di analisis secara deskriptif. Hasil penelitian ini adalah: (1) antecedents (input) penerapan K3 pada praktik finishing bangunan dalam aspek kesiapan guru dan siswa sudah baik, ditinjau dari penetapan dasar hukum dan perencanaan K3 sudah terlaksana. (2) transcription (process) penerapan K3 pada praktik finishing bangunan telah berjalan dengan baik dilihat dari persentase penerapan K3 sebanyak 96%. (3) output penerapan K3 pada praktik finishing bangunan ditinjau dari hasil Pemantauan dan evaluasi kinerja K3 yang dicapai sudah baik.