bluuebunny/crossref_metadata_2025_split huggingface dataset:
Detection of genes positively selected in Cuban Anolis lizards that naturally inhabit hot and open areas and currently thrive in urban areas
Authors: Kanamori Shunsuke , Cádiz Antonio , Díaz Luis M. , Ishii Yuu , Nakayama Takuro , Kawata Masakado 
Published: January 2021

AbstractSpecies of Anolis lizards of the West Indies that naturally inhabit hot and open areas also tend to thrive in urban areas. In this study, transcriptome was sequenced for nine species of Cuban Anolis lizards that are closely related to each other, but inhabit different thermal microhabitats. Using PAML and HyPhy software, we attempted to identify genes and amino acid sites under positive selection in the common ancestral branch of A. porcatus and A. allisoni, and the branch of A. sagrei, which inhabit hot and open areas, and thrive in urban areas. Although there were no genes where positive selection was commonly detected on both of the tested branches, positive selection was detected in genes involved in the stress response (e.g., DNA damage and oxidative stress) and cardiac function, which could be related to adaptive evolution of tolerance to heat or ultraviolet radiation, on both branches. These findings suggest that adaptive evolution of the response to stress caused by heat or ultraviolet radiation might have occurred in ancestors of Anolis species inhabiting hot and open areas and might be related to the current thriving in urban areas of them.
Effectiveness of a structured group intervention based on pain neuroscience education for patients with fibromyalgia in primary care: A multicentre randomized open‐label controlled trial
Authors: Barrenengoa‐Cuadra María Jesús , Muñoa‐Capron‐Manieux María , Fernández‐Luco Marian , Angón‐Puras Luis Ángel , Romón‐Gómez Ana J. , Azkuenaga Maider , Etxebarria Amaia , Orrantia Gixane , Pikaza Ainhoa , Uribe‐Etxebarria Lourdes , Zorrilla Ana , Larrinaga Gorka , Arana‐Arri Eunate , Gracia‐Ballarín Rafael ,   for the FIMIDOC Working Group researchers
Published: January 2021

AbstractBackgroundThere has been increased interest in pain neuroscience education (PNE) as a therapeutic approach for the management of fibromyalgia (FM).MethodsA multicentre randomized, open‐label, controlled trial was conducted to assess the effectiveness of a structured group intervention based on PNE in patients with FM. A total of 139 patients were included in the study and randomized to the intervention group (7 group sessions of education in neurobiology of pain) or to the control group (treatment as usual only). The primary outcome was the improvement of functional status and pain measured with the Fibromyalgia Impact Questionnaire (FIQ), and secondary outcomes were the reduction in the impact of pain and other symptoms (catastrophizing, anxiety and depression) and number of patients reaching no worse than moderate functional impairment (FIQ score <39). Differences between groups were calculated by linear mixed‐effects (intention‐to‐treat approach) and mediational models through path analyses.ResultsAt 1 year, improvements in FIQ scores were higher in the intervention group with moderate or high effect size, and decreases of ≥20% in 69.1% of patients (20.9% in the control group) and of ≥50% in 39.7% (4.5% in the control group). Also, 52.9% of patients had a FIQ <39 points (13.4% in the control group).ConclusionsIn this sample of patients with FM, the improvement in quality of life and control of symptoms obtained by adding a PNE intervention showed promising results, equalling or surpassing previously reported outcomes.SignificanceA structured group intervention based on pain neuroscience education for 1 year in patients with fibromyalgia was associated with significant amelioration of the impact of the disease on scores of the Fibromyalgia Impact Questionnaire, the Health Assessment Questionnaire, the Hospital Anxiety and Depression Scale, the Pain Catastrophizing Scale and the Polysymptomatic Distress Scale as compared with only treatment as usual. These findings are clinically relevant considering the challenges posed by fibromyalgia to clinicians and patients alike.
2D Accordion‐like MXene Nanosheets as a Sensitive Electrode Material for Baicalin Sensing
Authors: Shi Yan‐Mei , Zhang Xi , Mei Lin , Hu Kai , Chao Li‐Qin , Li Xiu‐Min , Miao Ming‐San 
Published: January 2021

AbstractHerein, accordion‐like MXene nanosheets was used as electrode sensing material for baicalin determination in traditional Chinese medicine preparation for the first time. The consecutive multilayer nanostructure of MXene observably enhanced the electroactive surface area of electrode and accelerated the electron transfer rate to acquire outstanding electrochemical response to baicalin. The electrode process of baicalin and relative dynamic parameters were investigated using cyclic voltammetry and chronocoulometry. Under optimized conditions, a good linear relationship was achieved between the response current and baicalin levels over the range of 0.08–60 μM with a detection limit of 26 nM (S/N=3). The developed sensor was successfully applied for baicalin monitoring in traditional Chinese medicine preparation and human urine samples.
Effect of blowing parameters on bath mixing efficiency during basic oxygen furnace steelmaking process
Authors: Wang Zhou , Liu Haitao , Wei Shizhong , Liu Qing , Lu Xinchun 
Published: January 2021

AbstractBasic Oxygen Furnace (BOF) steelmaking is the main method for steelmaking production, mixing efficiency of BOF vessel would greatly affect the uniformity of chemical composition and temperature in molten steel. Therefore, this paper conducted the effect of blowing parameters on bath mixing efficiency during BOF steelmaking process. Through measuring the mixing time of steel bath in BOF vessel, the effect of different blowing parameters was studied. Based on analysis of variance for the experimental results, the influence degree on mixing efficiency by different blowing parameters was acquired, and the optimized combination of blowing parameters was obtained. Moreover, the mixing index which indicated the mixing degree of steel bath in BOF vessel was proposed in this study, and an apparent nonlinear relation was acquired after determining relationship between the mixing index and blowing parameters. The optimal blowing parameters were applied to an 80 t industrial BOF vessel in a special steel plant, the end point carbon and oxygen equilibrium was decreased from 0.0032 to 0.0026, end point phosphorus and total Fe content in slag were reduced by 0.0012% and 2.21%, respectively. The mixing index and results presented in this work could be helpful to improve mixing efficiency in commercial BOF vessels.
High adsorption of methylene blue from water onto graphenic materials: Effect of degree of graphitization and analysis of kinetic models
Authors: Pérez‐Ramírez Eduardo E. , de la Luz‐Asunción Miguel , Martínez‐Hernández Ana L. , Luna‐Bárcenas Juan G. , García‐Casillas Perla E. , Velasco‐Santos Carlos 
Published: January 2021

AbstractThe removal by adsorption of methylene blue dye from water using two‐dimensional (2D) (graphene oxide and reduced graphene oxide) and three‐dimensional (3D) (graphite and graphite oxide) carbon structures was studied. Two graphites with different degrees of graphitization were used to evaluate the significance of the carbon structural order in the adsorption efficiency, and therefore, the economic repercussions, because the degree of graphitization is related with the cost. Graphene oxides showed the best performance in methylene blue adsorption, with a removal of around 90%. The adsorption process was strongly influenced by the surface oxygenated groups, and electrostatic interaction was the main mechanism of adsorption. The degree of graphitization of the precursor does not have a strong influence on the adsorption results of methylene blue. Thus, graphene oxide with a lower degree of graphitization represents an economical and efficient adsorbent for the removal of methylene blue from water. The kinetic study was realized in pseudo‐first order, pseudo‐second order, and Elovich kinetic models in their linear and non‐linear forms. According to the determination coefficient and the standard deviation, the experimental data were best fitted to the Elovich model in its non‐linear form.
Investigation on lithium ion battery equivalent circuit models for dynamic load profiles
Authors: Bairwa Bansi L. , Pareek Kapil , Hampannavar Santosh kumar 
Published: January 2021

AbstractTo achieve accurate prediction of the nonlinear behavior of lithium‐ion battery, parameters of the lithium‐ion battery model play a key role for dynamic performance, hence the parameters of the investigated battery model have been estimated. In this work, the electrical equivalent circuit (ECM) models have been used to simulate INR18650‐20R lithium‐ion battery response at 0°C, 25°C, and 45°C, respectively. The ECM model parameters are estimated using incremental open‐circuit voltage (OCV) profile and Trust‐Region Reflective algorithm by the Non‐Linear Least Squares method. The estimated parameters of the ECM model experimentally validated using independent dynamic current profiles including Dynamic Stress Test (DST), Federal urban driving schedule (FUDS), Beijing Dynamic Stress test (BJDST), and US06 at 0°C, 25°C, and 45°C, respectively. Coulomb‐counting based algorithms are implemented for model‐based online SoC estimation. The investigated ECM model with three parallel RC branches exhibits accuracy below 1.8% for different temperature levels and seems suitable to capture the nonlinear dynamics of lithium‐ion batteries for EV application. It is confirmed that the errors resulting from the investigated ECM model are significantly reduced comparatively existing published models.
An improved privacy preserving remote user authentication scheme for agricultural wireless sensor network
Authors: Rangwani Diksha , Sadhukhan Dipanwita , Ray Sangram , Khan Muhammad Khurram , Dasgupta Mou 
Published: January 2021

AbstractAgriculture is a fundamental pillar of society, bearing a crucial role in shaping the economy of the country and provides huge financial prospects and the possibility of employment to the large mass of the country. Thus, the incorporation of modern technology would lead to amplification in productivity and economic escalation. In this perspective, Wireless Sensor Network (WSN) can provide a greater service by sensing, monitoring and supplying continuous information about climatic conditions like temperature, light, soil moisture, humidity, and so on. These parameters help in increment of productivity in terms of quantity and quality growth in agriculture. However, WSN brings security threats like masquerading, alteration, interference, interception, and so on, that harmfully affect crop production and other agricultural practices. Thus, privacy preservation and security enhancement for agricultural WSN are the major challenges to be conquered. To meet this purpose, a privacy‐preserving efficient and secure remote user authentication scheme is introduced in this research work for the application of agricultural WSN. The proposed scheme is formally evaluated using probabilistic Random‐Oracle‐Model (ROR) for proving the robustness of the scheme. Further, the scheme is simulated using a well‐accepted AVISPA tool to show the security strength of it. The performance analysis of our scheme shows that along with its robustness in terms of security, the proposed scheme is more efficient in terms of computation and communication overheads in contrast to other existing schemes. These aforesaid features make the proposed scheme implementable in a practical environment.
Clinical validation of 13‐gene DNA methylation analysis in oral brushing samples for detection of oral carcinoma: Italian multicenter study
Authors: Gissi Davide B. , Morandi Luca , Colella Giuseppe , De Luca Roberto , Campisi Giuseppina , Mauceri Rodolfo , Romeo Umberto , Tenore Gianluca , Mignogna Michele D. , Adamo Daniela , Oteri Giacomo , Marcianò Antonia , Vescovi Paolo , Meleti Marco , Serpico Rosario , Di Stasio Dario , Pentenero Monica , Santarelli Andrea , Bianchi Alberto , Crimi Salvatore , Marchetti Claudio , Tarsitano Achille , Foschini Maria Pia , Montebugnoli Lucio 
Published: January 2021

AbstractBackgroundThe aim of this Italian multicenter study was to evaluate the diagnostic performance of a minimally invasive method for the detection of oral squamous cell carcinoma (OSCC) based on 13‐gene DNA methylation analysis in oral brushing samples.MethodsOral brushing specimens were collected in 11 oral medicine centers across Italy. Twenty brushing specimens were collected by each center, 10 from patients with OSCC, and 10 from healthy volunteers. DNA methylation analysis was performed in blindness, and each sample was determined as positive or negative based on a predefined cutoff value.ResultsDNA amplification failed in 4 of 220 (1.8%) samples. Of the specimens derived from patients with OSCC, 93.6% (103/110) were detected as positive, and 84.9% (90/106) of the samples from healthy volunteers were negative.ConclusionThese data confirmed the diagnostic performance of our novel procedure in a large cohort of brushing specimens collected from 11 different centers and analyzed in blindness.
Comparison of in vitro fertilization cycles stimulated with 20 mg letrozole daily versus high‐dose gonadotropins in Rotterdam Consensus ultra‐poor responders: A proof of concept
Authors: Khojah Mohammad , Khayat Suhaib , Dahan Michael H. 
Published: January 2021

AbstractObjectiveTo evaluate if high‐dose letrozole can be used successfully to stimulate poor responders for in vitro fertilization (IVF).MethodsThis was a retrospective study conducted at a university hospital reproductive center. The analysis included women who were up to 42 years of age and were Rotterdam Consensus poor responders. A total of 247 patients received gonadotropins (300–450 IU daily) and 62 patients were stimulated with letrozole (20 mg daily) as part of an antagonist IVF protocol.ResultsThe use of 20 mg of letrozole decreased the total dose of gonadotropins used (645 ± 175 IU vs. 5360 ± 1028 IU, P = 0.001) and resulted in lower costs of stimulation medications ($ 555.56 ± $ 150 vs. $ 4616 ± $ 885 Canadian Dollars; P = 0.001). Pregnancy per cycle (14.5%) and per transfer (16%) rates were legitimate for this low prognosis group and may have been better than or similar to those with high‐dose gonadotropins. The rate of cycle cancellation may have been reduced in the letrozole versus gonadotropin group (11% vs. 38%; P = 0.001).ConclusionLetrozole (20 mg daily) may be used to reduce the cost of ovarian stimulation in ultra‐poor responders, significantly reducing the cost of the IVF cycle with probably at least similar outcomes to high‐dose gonadotropins.
PD‐L1 overexpression conveys tolerance of mesenchymal stem cell‐derived cardiomyocyte‐like cells in an allogeneic mouse model
Authors: Mardomi Alireza , Limoni Shabanali K. , Rahbarghazi Reza , Mohammadi Nabiallah , Khorashadizadeh Mohsen , Ranjbaran Hossein , Nataj Hadi H. , Jafari Narjes , Hasani Bahareh , Abediankenari Saeid 
Published: January 2021

AbstractAlthough the autologously transplanted cells are immunologically durable, allogeneic cell transplantation is inevitable in a series of cases. Mesenchymal stem cells (MSCs) are one of the suitable candidates for cardiac tissue regeneration that have been shown to acquire immunogenicity concurrent with cardiomyogenic differentiation. The present study aimed to exploit PD‐L1, as a key immunomodulatory checkpoint ligand to protect the MSCs‐derived cardiomyocyte‐like cells (CLCs) against the detrimental alloimmunity. Mouse bone marrow‐derived MSCs were stably transduced to overexpress PD‐L1. MSCs were in vitro differentiated into CLCs and the expressions of immunologic molecules were compared between MSCs and CLCs. The in vitro and in vivo allogeneic immune responses were also examined. The differentiated CLCs had higher expressions of MHC‐I and CD80. Upon in vitro coculture with allogeneic splenocytes, CLCs caused more CD4+ and CD8+ T cell activation, lymphocyte proliferation, and interferon‐γ (IFN‐γ) release in comparison to MSCs. PD‐L1 overexpression on CLCs decreased the activation of CD8+ T cells, proliferation of lymphocytes, and release of IFN‐γ. The PD‐L1‐overexpressing CLCs elicited lower in vivo CD4+ and CD8+ T cell activation and reduced the anti‐donor antibody response accompanied by increased durability and reduced T cell infiltration. The present study verified the potential of PD‐L1 overexpression as a preparative strategy for the protection of allogeneic MSCs‐derived CLCs against the detrimental alloreaction.
Association between the chronic use of gastric acid suppressants and high‐risk colorectal polyps
Authors: Shiratori Yasutoshi , Ikeya Takashi , Ishii Naoki , Yamamoto Kazuki , Honda Tetsuro , Hasatani Kenkei , Yoshida Naohiro , Nishida Tsutomu , Sumiyoshi Tetsuya , Kiyotoki Shu , Arai Masahiro , Niikura Ryota 
Published: January 2021

AbstractBackground and AimAlthough gastric acid suppressants such as proton pump inhibitors (PPIs) and histamine‐2 receptor antagonists (H2RAs) are considered safe, the consequences of hypochlorhydria and hypergastrinemia caused by chronic use are unclear. This study aimed to investigate the association between the chronic use of gastric acid suppressants and high‐risk colorectal polyps, focusing on polyp size.MethodsA population‐based, nested case–control study was conducted using data from the Japanese Diagnosis Procedure Combination database between 2014 and 2019. Cumulative PPI or H2RA use prior to polypectomy was evaluated during the study period. Endoscopic polypectomy was categorized as polypectomy <2 cm, polypectomy ≥2 cm, and endoscopic submucosal dissection. Baseline characteristics were compared between the high‐risk (≥2 cm polyps or polyps treated by endoscopic submucosal dissection) and low‐risk (<2 cm polyps) endoscopic polypectomy groups. We calculated adjusted odds ratios (ORs) using multivariable logistic regression analysis.ResultsOf 27 694 patients who underwent endoscopic polypectomy, 2518 were treated with PPIs or H2RAs for >1 year prior to polypectomy. After adjusting for age, gender, and other confounders, a higher prevalence of high‐risk colorectal polyps was noted with PPI (OR: 2.67; 95% confidence interval: 2.37–3.01) and H2RA (OR: 1.86; 95% confidence interval: 1.52–2.26) use. Longer PPI or H2RA use was associated with increased risks of high‐risk colorectal polyps (P for trend <0.001). The highest OR (3.17) was observed among patients who received PPIs for ≥3 years.ConclusionChronic use of PPIs and H2RAs may be associated with high‐risk colorectal polyps. Requirements for long‐term gastric acid suppressant use should be reevaluated.
Simple and green method for synthesis of new diastereoselective spiroheterocycles catalyzed by copper ferrite
Authors: Ghanbari Zahra , Naeimi Hossein 
Published: January 2021

AbstractSome new spirocarbocyclic compounds with highly diastereoselectivity were synthesized using a simple, green and high efficiency method. The IR, 1H NMR and 13C NMR spectroscopy and C.H.N analyses were applied for identification of these compounds. This novel synthetic approach was performed from acid catalytic pathway using CuFe2O4 MNPs as a nanocatalyst in aqueous moiety.
On‐tissue boronic acid derivatization for the analysis of vicinal diol metabolites in maize with MALDI‐MS imaging
Authors: Forsman Trevor T. , Dueñas Maria Emilia , Lee Young Jin 
Published: January 2021

AbstractDerivatization reactions are commonly used in mass spectrometry to improve analyte signals, specifically by enhancing the ionization efficiency of those compounds. Vicinal diols are one group of biologically important compounds that have been commonly derivatized using boronic acid. In this study, a boronic acid with a tertiary amine was adapted for the derivatization of vicinal diol metabolites in B73 maize tissue cross‐sections for mass spectrometry imaging analysis. Using this method, dozens of vicinal diol metabolites were derivatized, effectively improving the signal of those metabolites. Many of these metabolites were tentatively assigned using high‐resolution accurate mass measurements. In addition, reaction interference and cross‐reactivity with various other functional groups were systematically studied to verify data interpretation.
2021 ISHNE/HRS/EHRA/APHRS collaborative statement on mHealth in Arrhythmia Management: Digital Medical Tools for Heart Rhythm Professionals
Authors: Varma Niraj , Cygankiewicz Iwona , Turakhia Mintu , Heidbuchel Hein , Hu Yufeng , Chen Lin Yee , Couderc Jean‐Philippe , Cronin Edmond M. , Estep Jerry D. , Grieten Lars , Lane Deirdre A. , Mehra Reena , Page Alex , Passman Rod , Piccini Jonathan , Piotrowicz Ewa , Piotrowicz Ryszard , Platonov Pyotr G. , Ribeiro Antonio Luiz , Rich Robert E. , Russo Andrea M. , Slotwiner David , Steinberg Jonathan S. , Svennberg Emma 
Published: January 2021

AbstractThis collaborative statement from the International Society for Holter and Noninvasive Electrocardiology/Heart Rhythm Society/European Heart Rhythm Association/Asia Pacific Heart Rhythm Society describes the current status of mobile health (“mHealth”) technologies in arrhythmia management. The range of digital medical tools and heart rhythm disorders that they may be applied to and clinical decisions that may be enabled are discussed. The facilitation of comorbidity and lifestyle management (increasingly recognized to play a role in heart rhythm disorders) and patient self‐management are novel aspects of mHealth. The promises of predictive analytics but also operational challenges in embedding mHealth into routine clinical care are explored.
Divergent effects of distinct perivascular cell subsets for intra‐articular cell therapy in posttraumatic osteoarthritis
Authors: Hsu Ginny Ching‐Yun , Cherief Masnsen , Sono Takashi , Wang Yiyun , Negri Stefano , Xu Jiajia , Peault Bruno , James Aaron W. 
Published: January 2021

AbstractIntra‐articular injection of mesenchymal stem cells has shown benefit for the treatment of osteoarthritis (OA). However, mesenchymal stem/stromal cells at the origin of these clinical results are heterogenous cell populations with limited cellular characterization. Here, two transgenic reporter mice were used to examine the differential effects of two precisely defined perivascular cell populations (Pdgfrα+ and Pdgfrβ+ cells) from white adipose tissue for alleviation of OA. Perivascular mesenchymal cells were isolated from transgenic Pdgfrα‐and Pdgfrβ‐CreERT2 reporter animals and delivered as a one‐time intra‐articular dose to C57BL/6J mice after destabilization of the medial meniscus (DMM). Both Pdgfrα+ and Pdgfrβ+ cell preparations improved metrics of cartilage degradation and reduced markers of chondrocyte hypertrophy. While some similarities in cell distribution were identified within the synovial and perivascular spaces, injected Pdgfrα+ cells remained in the superficial layers of articular cartilage, while Pdgfrβ+ cells were more widely dispersed. Pdgfrβ+ cell therapy prevented subchondral sclerosis induced by DMM, while Pdgfrα+ cell therapy had no effect. In summary, while both cell therapies showed beneficial effects in the DMM model, important differences in cell incorporation, persistence, and subchondral sclerosis were identified.