bluuebunny/crossref_metadata_2025_split huggingface dataset:
Self-floating Capsule of Algicidal Bacteria Bacillus sp. HL and its Performance in the Dissolution of Microcystis Aeruginosa
Authors: Huang Jinjie , Zhang Wenyi , Xu Mingchen , Mao Linqiang 
Published: September 2021

Abstract
Algicidal bacteria is known as efficient and environmentally friendly in treating Microcystis aeruginosa (M. aeruginosa). However, the practical application of algicidal bacteria in the natural water is limited by the interference of external factors and the low reuse capability. In this study, a biodegradation capsule for M. aeruginosa is prepared by biocompatible sodium alginate (SA) compositing with eco-friendly ethyl cellulose (EC). Bacterial strain HL was immobilized and the capsule was obtained under optimal usage concentrations of SA, Calcium chloride (CaCl2) and EC at 2%, 3% and 3%. It has been observed that capsules immobilizing bacteria HL shows considerable advantages over traditional bio-treatment systems (free-living bacteria) and good reusability performance. A better dissolution rate of 77.67% ± 1.14% on the 7th day was obtained with the embedding of algicidal bacteria at 50 mL, which enhanced algae dissolution rate by 11.05% compared with free-living bacteria, and the dissolution rate for M. aeruginosa still reaches 68.57% ± 2.88% after three times repetitive use. Algicidal bacteria capsules were examined on the fluorescence and antioxidant system of M. aeruginosa. It was indicated that photosynthetic mechanisms of M. aeruginosa were destroyed, superoxide dismutase (SOD), peroxidase (POD), catalase (CAT) were all significantly induced as antioxidant response, and malondialdehyde (MDA) content increased. Overall, capsules prepared in this study can provide a desirable environment for algicidal bacteria HL and ensure algicidal bacteria to in-situ work well in the inhibition of algae.
Comparison of Extended Segmentectomy with Traditional Segmentectomy for Stage I Lung Cancer
Authors: Cheng Ya-Fu , Hsieh Yueh-Che , Chang Yu-Jun , Cheng Ching-Yuan , Huang Chang-Lun , Hung Wei-Heng , Wang Bing-Yen 
Published: September 2021

Abstract
Background
For stage I non-small cell lung cancer (NSCLC), lobectomy and segmentectomy are still controversial operations. Extended segmentectomy was proposed to make larger safe margins than segmentectomy. Image-guided video-assisted thoracoscopic surgery (iVATS) is useful to accomplish extended segmentectomy. We aimed to compare the effects of iVATS extended segmentectomy to the effects of traditional segmentectomy for stage I NSCLC.
Methods
This study is a retrospective analysis in a single institute. Patients with stage I NSCLC who received segmentectomy between January 2017 and September 2020 were included. Patients were distributed to iVATS extended segmentectomy (group A), and traditional segmentectomy (group B). The impacts of the different surgical methods on resection margin were assessed.
Results
There were 116 patients enrolled in this study. Sixty-two patients distributed in group A, and the other 54 patients in group B. The resection margin to a staple line was 17.94 mm in group A versus 14.15 mm in group B, p = 0.037. The margin/tumor diameter ratio was 2.08 in group A versus 1.39 in group B, p = 0.003. The enough margin rate was 75.81% and 57.41%, respectively, for group A and group B. The subgroup analysis of iVATS extended segmentectomy showed that T1a lesions had larger margin distances than did T1b lesions (19.85 mm vs. 14.83 mm, p = 0.026).
Conclusions
The iVATS extended segmentectomy can provide more resection margin than traditional segmentectomy. Segmentectomy is more suitable to perform when the nodule’s diameter is less than 10 mm.
Wrist Actimetry Biomarker Development of Paretic Upper Limb Use in Post Stroke Patients for Ecological Monitoring.
Authors: Dusfour Gilles , Mottet Denis , Muthalib Makii , Laffont Isabelle , Bakhti Karima K.A. 
Published: September 2021

Abstract
Background

In post-stroke patients it is unclear which wrist actimetry biomarkers to use to estimate the degree of upper limb hemiparesis. The objective of this study was to develop a general and objective framework for monitoring hemiparetic patients in their home environment via different biomarkers based on 7 days of actimetry data. A secondary objective was to use all of these biomarkers to better understand the mechanism for potential non-use of the paretic upper limb.
Methods

Accelerometers were worn continuously for a period of 7 days on both wrists of 10 post-stroke hemiparetic patients as well as 6 healthy subjects. Various wrist actimetry biomarkers were calculated, including the Jerk ratio 50 (JR50, cumulative probability that the Jerk Ratio is between 0 and 0.5), absolute and relative amounts of functional use of movements of the upper limbs (FuncUse and FuncUseR) and absolute and relative velocities of the upper limbs during functional use (VUL and VULR). For each biomarker, the values of stroke and healthy groups were compared. The correlations between all the biomarkers were studied.
Results

We studied 10 participants with mild-to-moderate chronic hemiparesis and 6 healthy control participants. FuncUse and VUL of the paretic upper limb of stroke patients were significantly lower than in the non-dominant upper limb of healthy subjects. Similarly, FuncUseR (paretic/non-paretic vs non-dominant/dominant), JR and VULR are significantly lower in stroke patients than in healthy subjects. FuncUseR, VULR and JR50 seem to be complementary biomarkers for monitoring patient strokes.
Conclusion

The stroke patients do not seem to compensate for the decrease in functional movement on the paretic side by an increase on the non-paretic side. The speed of execution of functional movements on the paretic side could be the limiting factor to a normal use of the paretic upper limb. A thorough clinical study is needed to identify the limiting factors. In conclusion, this study for the first time has shown actimetry is a robust and non-obtrusive lightweight technology for continuously acquiring objective upper limb data of paretic arm use/ non-use over an extended period in a home environment for monitoring stroke patients.
Characteristic of Weld Bead By Using Flat Wire Electrode In GMAW Inline During The Process: An Experimental And Numerical Analysis
Authors: Sripri K , Ramu M , Thyla P R , Anantharuban K , Karthigha M 
Published: September 2021

Abstract
This paper focusses on effect of flat wire electrode on gas metal arc welding (FW-GMAW) inline during the process to improve quality of weld bead. Since the flat wire electrode perimeter has enlarged, the turbulence of the molten droplet characteristics is altered due to the effect of electromagnetic force. To bring out, butt joint welding are proven with use of the flat wire electrode. The experimental results indicated that, the flat wire electrode and their orientations are significantly factors to improve the weld bead dimensions, due to the increased in steady state temperature and heat density. The end result also evident that the depth-to-width (D/W) ratio was improved on an average by 7.7 % than regular wire electrode used in GMAW. To validate, a comprehensive numerical model was developed, the result confirmed that the initial arc and steady state temperature are high for FW-GMAW. In addition, improvements in metallurgical and mechanical properties are discussed in this paper.
Maturation of Nephrons by Implanting hPSC-Derived Kidney Progenitors Under Kidney Capsules of Unilaterally Nephrectomized Mice
Authors: Yu Xin , Jiang Shan , Li Kailin , Yang Xianzhen , Xu Zhihe , Du Xiaohang , Kong Feng , Zhao Shengtian 
Published: September 2021

Abstract
Background

Human pluripotent stem cell (hPSCs)-derived kidney organoids may contribute to disease modeling and generation of kidney replacement tissues. However, realization of such applications requires the induction of hPSCs into functional mature organoids. One of the key questions for this process is whether a specific vascular system exists for nephrogenesis. Our previous study showed that implantation of hPSC-derived organoids below the kidney capsules of unilaterally nephrectomized immunodeficient mice for a short-term (2 weeks) resulted in the enlargement of organoids and production of vascular cells, although signs of maturation were lacking.
Methods

In this study, organoids are induced in vitro during 15 days and then sub-capsularly grafted into kidneys, we used the same unilaterally nephrectomized immunodeficient mice model to examine whether a medium -term (4 weeks) implantation could improve organoid maturation and vascularization, as evaluated by immunofluorescence and transmission electron microscopy(TEM).
Results

We demonstrate that after 2–4 weeks implantation, implanted renal organoids can form host-derived vascularization and mature in the absence of any exogenous vascular endothelial growth factor. Glomerular filtration barrier maturation was evidenced by glomerular basement membrane deposition, perforated glomerular endothelial cell development, as well as apical to basal podocyte polarization. A polarized monolayer epithelium and extensive brush border were also observed for tubular epithelial cells.
Conclusions

Our results indicate that the in vivo microenvironment is important for the maturation of human kidney organoids. Stromal expansion and a reduction of nephron structures were observed following longer-term (12 weeks) implantation,suggesting effects on off-target cells during the induction process. Accordingly, induction efficiency and transplantation models should be improved in the future.
The Effect of Season of Birth On The Morphometrics of Newborn Belgian Blue Calves
Authors: Tuska Habib Syaiful Arif , Residiwati Gretania , Eetvelde Mieke Van , Verdru Karel , Meesters Maya , Budiono  , Pascottini Osvaldo Bogado , Soom Ann Van , Opsomer Geert 
Published: September 2021

Abstract
Breed type and environmental factors such as breeding season may have a significant impact on neonatal weight loss calf size. We followed a total of 236 elective cesarean sections in Belgian Blue (BB) cattle, in which neonatal calves were morphometrically measured (in cm) in the first 72 hours after delivery of the child using a strictly standardized protocol. The influence of the season of birth on each calf measurement was analyzed using a mixed linear regression models, including farm of origin as a random effect. Calves born in spring had a longer diagonal length (P = 0.05) (69.7 ± 1.24) than those born in autumn (66.9 ± 1.16). The tibial length of calves born in spring (35.8 ± 0.48) was longer (P> 0.02) than those born in autumn (33.1 ± 0.57) or summer (34.1 ± 0.49). Calves born in autumn have a shorter head diameter (P> 0.02) (12.9 ± 0.23) than those born in summer (12.6 ± 0.29) or winter (13.5 ± 0.22). For all other parameters, no differences were found (P> 0.08). Based on the results of this study, it can be concluded that the birth season influences the morphometrics of neonatal BB calves, with a tendency for spring to be associated with the largest body size. The latter is important to know to avoid dystocia when BB cattle are crossed with other breeds.
Analysis and Source Identification of Heavy Metals in Soils of Central Urban Area of Chongqing, China
Authors: Chen Wende , Zhu Kun , Cai Yankun , Peng Peihao 
Published: September 2021

Abstract
In megacities, due to frequent human activities, large amounts of metals enter the soil indirectly or directly and eventually flow to people through the food chain. Therefore, the analysis and identification of soil heavy metal sources is an important part of revealing soil heavy metal pollution. The spatial content and potential sources of 11 heavy metals were analyzed from 342 surface soil samples collected from the central city of Chongqing in southwest China. The results showed that the main heavy metal elements under the first principal component loading were copper (Cu), nickel(Ni), zinc (Zn), manganese (Mn), cadmium (Cr), plumbum (Pb) and cadmium (Cd). The second principal component (F2) was mainly loaded with molybdenum (Mo), arsenic (As), mercury (Hg) and antimony (Sb), and the PCA-APCs receptor model of 11 heavy metals was constructed. The PCA-APCs receptor models of 11 heavy metals were constructed. The results of classification regression analysis confirmed the main sources of heavy metals. Population density mainly affected Cu (0.539), soil mainly affected Ni (0.411), Sb (0.493), Zn (0.472) and Mn (0.206), and water quality mainly affected As (0.453) and Mo (0.374). Air quality mainly affects Cd (0.332) and Cr (0.371), traffic activity mainly affects Hg (0.312), and slope mainly affects Pb (0.313). Hot spot analysis showed that heavy metals had a high degree of coincidence with environmental factors such as soil parent material, slope, soil type and traffic activities. The results of this study can be effectively used to make scientific decisions and strategies, and an effective strategy for prevention and control of soil heavy metal pollution should be formulated to protect the urban soil environmental quality.
Comparision Study On The Knowledge and Attitude Between Healthcare Workers Based On Educational materials of Nucleotie Based Vaccines
Authors: Madadha M. Emad Al , Ahmed Khalid E. , Rayyan Rama , Ahram Mamoun , Al-Sanouri Nancy , Demour Saddam Al , Ahmad Muayyad 
Published: September 2021

Abstract
Background
With the implementation of nucleotide-based vaccines (NBVs) in the COVID-19 vaccination campaigns, a wide controversy surrounding NBVs has become a heated subject of debate, and it did not spare healthcare workers (HCW) and staff. HCW have a powerful influence on the acceptance of NBVs by the general public. Hence, the aim of this study was to assess the knowledge and attitude of healthcare workers regarding this new vaccine technology.
Methods
This is a cross-sectional study using an online survey involving health workers in Jordan. The survey assessed the participants' socio-demographic characteristics, knowledge, and attitude about the safety and efficacy if NBVs. The study population was divided into two groups, educated and uneducated groups, whereby the former received a small educational pamphlet on NBVs. The Mann-Whitney test was used to compare between the response of the two groups.
Results
A total of 330 health workers participated in this study. Respondents believed that RNA-based NBVs would be a safer option compared to DNA-based ones, with the educated group showing significant difference. The notion of NBVs being a form of gene therapy was more common among the educated group. The majority of the participants, particularly amongst the uneducated group, were concerned about undiscovered effects of NBVs. Respondents also agreed that NBVs must be reevaluated in phase 1 trials. As for efficacy, our study population agreed that these vaccines would prevent severe illness.
Conclusions
Although healthcare workers have positive knowledge and attitude towards NBVs, misconceptions and skepticism exist and must be addressed with more education efforts.
Preliminary Study of the Significance of Reverberation by IVUS Detection for Patients With Severe Calcified Lesions
Authors: You Wei , Zhang Hong-li , Xu Tian , Meng Pei-na , Zhou Yu-he , Wu Xiang-qi , Wu Zhi-ming , Tao Bi-lin , Guo Ya-jie , Nong Jia-cong , Ye Fei 
Published: September 2021

Abstract
Objectives―To explore the potential significance of the reverberation of calcification by comparing both intravascular ultrasound (IVUS) and optical coherence tomography (OCT) measurement post manual coregistration.Background―The reverberation phenomenon is often detected by IVUS for severe calcified lesions post rotational atherectomy (RA), which is thought to be due to the glassy and smooth inner surfaces of calcifications. Because of the poor penetration of IVUS, it is impossible to measure the thickness of calcifications, and the relationship between multiple reverberations and the thickness of calcification lesions has not been reported before.Methods―A total of forty-nine patients with severe calcified coronary lesions that were detected by IVUS and OCT simultaneously were enrolled in our retrospective study. If reverberation phenomena were detected by IVUS, intravascular imaging (IVI) data (including distance between the IVUS catheter center and the inner surface of the reverberation signal, the intervals between all adjacent reverberation signals, the number of layers of reverberation in IVUS, and the thickness of the calcification in OCT) were measured at the same position and same direction (each cross-section had 4 mutually perpendicular directions) at 1-mm intervals. If the observational point (direction) had a reverberation signal, it was an effective observational point that would be enrolled in our study; otherwise, it would be regarded as an invalid observational point and excluded from this study. The correlation between each reverberation observational value and OCT data was the primary target in this retrospective study. Results―Four hundred twenty-eight valid observational points were analyzed simultaneously by IVUS and OCT; among them, 300 points had a single layer of reverberation, 83 had double layers of reverberation and 42 had multiple layers (≥3 layers) of reverberation by IVUS detection post-RA. Multivariate logistic regression analysis showed that the number of layers of reverberation by IVUS was significantly related to the thickness of calcifications by OCT at the same point and in the same direction (p<0.001). Single, double, and multiple layers of reverberation in IVUS correspond to median calcification thicknesses (interquartile ranges (IQRs)) of 0.620 mm (0.520–0.720), 0.950 mm (0.840–1.040) and 1.185 mm (1.068–1.373), respectively, by OCT detection. No correlation was found between the distance between the IVI catheter center and the inner surface of the reverberation signal or the interval between every adjacent reverberation signal and the thickness of the calcification.Conclusions―The number of layers of reverberation signal detected by IVUS is positively correlated with the thickness of calcifications measured by OCT post-RA.
Molecular Detection and Prevalence of Non-O157 Shiga Toxigenic Escherichia Coli in Selected Chicken Abattoirs and Retail Outlets in Osogbo, Osun State Nigeria
Authors: Ayoade Femi , Oguzie Judith , Akano Kazeem , Olumade Testimony , Eromon Philomena , Odesola Kanyinsola , Alakija Oreoluwa , Folarin Onikepe , Happi Christian 
Published: September 2021

Abstract
Three selected chicken abattoirs and two retail locations were studied to determine the prevalence and profile for Shiga toxin-producing Escherichia coli (STEC) present in abattoirs and retail (frozen) chicken carcasses in Osun state, Nigeria. Samples were plated on differential and selective media for the presence of E. coli. Multiplex PCR was used to check for specific virulence factors in the isolated E. coli samples. The mean colony count results showed that effluent water samples from the Ikirun slaughter slab type abattoir were the highest at 25 cfu/ml. A post hoc comparison showed that this value was significantly higher than that of the slaughtering table at Oluode-1 (P = 0.04) and retail chicken meat samples at Igbona (P = 0.01). These results suggest inadequate waste management and biological waste disposal systems and guides on best practices to limit the spread of potentially virulent pathogens into the runoff and groundwater.
Longitudinal Axial Flow Rice Thresher Feeding Rate Monitoring Based On Force Sensing Resistors
Authors: Abdeen Mohamed Anwer , Xie Gan , Fu Jianwei , Salem Abouelnadar Elsayed , Zhang Guozhong 
Published: September 2021

Abstract
The threshing unit is the main working unit of the combine harvester and plays an essential role in rice threshing efficiency, seed loss, and seed damage. Every thresher has its limitation for feeding quantity, and when the feeding quantity exceeds the maximum rated amount, the thresher gets blocked, which results in higher losses, low threshing efficiency, more power consumption, and combine overloading, then combine shutting down at the end. In this study, a longitudinal axial flow rice threshing platform has been constructed, and a stress monitoring system for the top cover of the threshing drum was designed using force sensing resistors. The sensors were installed on the thresher top cover inner surface to detect the impact and extrusion forces caused by the threshing process and detect the feeding rate when it exceeds the suitable feeding of the combine. Three feeding rates (0.8, 1.1, and 1.4 kg/s) and three thresher speeds (1100, 1300, and 1500 rpm) were tested. The time of the testing process was calculated using high-speed photography. The obtained results revealed that the force signals collected by thin-film sensors significantly correlated with thresher rotating speed and feeding rate. This study provides a new method for threshing drum real-time feeding quantity monitoring and early warning of thresher blockage.
Comparison of Liver Scintigraphy and the Liver-Spleen Contrast in Gd-EOB-DTPA-Enhanced MRI on Liver Function Tests
Authors: Mori Hiroshige , Machimura Hanaka , Iwaya Amika , Baba Masaru , Furuya Ken 
Published: September 2021

Abstract
The liver-spleen contrast (LSC) using hepatobiliary-phase images could replace the receptor index (LHL15) in liver scintigraphy; however, few comparative studies exist. This study aimed to verify the convertibility from LSC into LHL15. In 136 patients, the LSC, not at 20 min, but at 60 min after injecting gadolinium-ethoxybenzyl-diethylenetriaminepentaacetic acid was compared with the LHL15, albumin–bilirubin (ALBI) score, and the related laboratory parameters. The LHL15 was also compared with their biochemical tests. The correlation coefficients of LSC with LHL15, ALBI score, total bilirubin, and albumin were 0.740, − 0.624, − 0.606, and 0.523 (P < 0.00001), respectively. The correlation coefficients of LHL15 with ALBI score, total bilirubin, and albumin were − 0.647, − 0.553, and 0.569 (P < 0.00001), respectively. The linear regression equation on the estimated LHL15 (eLHL15) from LSC was eLHL15 = 0.460 • LSC + 0.727 (P < 0.00001) and the coefficient of determination was 0.548. Regarding a contingency table using imaging-based clinical stage classification, the degree of agreement between eLHL15 and LHL15 was 65.4 %, and Cramer's V was 0.568 (P < 0.00001). Therefore, although the LSC may be influenced by high total bilirubin, the eLHL15 can replace the LSC as an index to evaluate liver function.