bluuebunny/crossref_metadata_2025_split huggingface dataset:
Ten emerging SARS-CoV-2 spike variants exhibit variable infectivity, animal tropism, and antibody neutralization
Authors: Zhang Li , Cui Zhimin , Li Qianqian , Wang Bo , Yu Yuanling , Wu Jiajing , Nie Jianhui , Ding Ruxia , Wang Haixin , Zhang Yue , Liu Shuo , Chen Zhihai , He Yaqing , Su Xiaodong , Xu Wenbo , Huang Weijin , Wang Youchun 
Published: October 2021

AbstractEmerging mutations in SARS-CoV-2 cause several waves of COVID-19 pandemic. Here we investigate the infectivity and antigenicity of ten emerging SARS-CoV-2 variants—B.1.1.298, B.1.1.7(Alpha), B.1.351(Beta), P.1(Gamma), P.2(Zeta), B.1.429(Epsilon), B.1.525(Eta), B.1.526-1(Iota), B.1.526-2(Iota), B.1.1.318—and seven corresponding single amino acid mutations in the receptor-binding domain using SARS-CoV-2 pseudovirus. The results indicate that the pseudovirus of most of the SARS-CoV-2 variants (except B.1.1.298) display slightly increased infectivity in human and monkey cell lines, especially B.1.351, B.1.525 and B.1.526 in Calu-3 cells. The K417N/T, N501Y, or E484K-carrying variants exhibit significantly increased abilities to infect mouse ACE2-overexpressing cells. The activities of furin, TMPRSS2, and cathepsin L are increased against most of the variants. RBD amino acid mutations comprising K417T/N, L452R, Y453F, S477N, E484K, and N501Y cause significant immune escape from 11 of 13 monoclonal antibodies. However, the resistance to neutralization by convalescent serum or vaccines elicited serum is mainly caused by the E484K mutation. The convalescent serum from B.1.1.7- and B.1.351-infected patients neutralized the variants themselves better than other SARS-CoV-2 variants. Our study provides insights regarding therapeutic antibodies and vaccines, and highlights the importance of E484K mutation.
Networked configurations as an emergent property of transverse aeolian ridges on Mars
Authors: Nagle-McNaughton T. P. , Scuderi L. A. 
Published: October 2021

AbstractTransverse aeolian ridges – enigmatic Martian features without a proven terrestrial analog – are increasingly important to our understanding of Martian surface processes. However, it is not well understood how the relationships between different ridges evolve. Here we present a hypothesis for the development of complex hexagonal networks from simple linear forms by analyzing HiRISE images from the Mars Reconnaissance Orbiter. We identify variable morphologies which show the presence of secondary ridges, feathered transverse aeolian ridges and both rectangular and hexagonal networks. We propose that the formation of secondary ridges and the reactivation of primary ridge crests produces sinuous networks which then progress from rectangular cells towards eventual hexagonal cells. This morphological progression may be explained by the ridges acting as roughness elements due to their increased spatial density which would drive a transition from two-dimensional bedforms under three-dimensional flow conditions, to three-dimensional bedforms under two-dimensional flow conditions.
Efficacy and Safety of Aspirin for Primary Cardiovascular Risk Prevention in Younger and Older Age: An Updated Systematic Review and Meta-analysis of 173,810 Subjects from 21 Randomized Studies
Authors: Calderone Dario , Greco Antonio , Ingala Salvatore , Agnello Federica , Franchina Gabriele , Scalia Lorenzo , Buccheri Sergio , Capodanno Davide 
Published: October 2021

Abstract
Aims The efficacy and safety of aspirin for primary cardiovascular disease (CVD) prevention is controversial. The aim of this study was to investigate the efficacy and safety of aspirin in subjects with no overt CVD, with a focus on age as a treatment modifier.
Methods and Results Randomized trials comparing aspirin use versus no aspirin use or placebo were included. The primary efficacy outcome was all-cause death. The primary safety outcome was major bleeding. Secondary ischemic and bleeding outcomes were explored. Subgroup analyses were conducted to investigate the consistency of the effect sizes in studies including younger and older individuals, using a cut-off of 65 years. A total of 21 randomized trials including 173,810 individuals at a mean follow-up of 5.3 years were included. Compared with control, aspirin did not reduce significantly the risk of all-cause death (risk ratio: 0.96; 95% confidence interval: 0.92–1.00, p = 0.057). Major adverse cardiovascular events were significantly reduced by 11%, paralleled by significant reductions in myocardial infarction and transient ischemic attack. Major bleeding, intracranial hemorrhage, and gastrointestinal bleeding were significantly increased by aspirin. There was a significant age interaction for death (p for interaction = 0.007), with aspirin showing a statistically significant 7% relative benefit on all-cause death in studies including younger patients.
Conclusion The use of aspirin in subjects with no overt CVD was associated with a neutral effect on all-cause death and a modest lower risk of major cardiovascular events at the price of an increased risk in major bleeding. The benefit of aspirin might be more pronounced in younger individuals.
Atherosclerotic Renal Artery Stenosis: A Review
Authors: Gunawardena Thilina 
Published: October 2021

AbstractRenal artery stenosis (RAS) is associated with hypertension and renal impairment. Atherosclerosis is the leading etiologic factor which accounts for >90% of the cases. Those with atherosclerotic RAS (ARAS) tend to have concomitant atherosclerosis in other vascular beds, so they are at a high risk of adverse coronary and cerebrovascular events. Management of ARAS is controversial, with limited indications for revascularization. In this review, the author aims to discuss the pathophysiology, natural history, diagnosis, and management of ARAS.
Total Laparoscopic Nerve-Sparing Radical Hysterectomy Using the No-look No-touch Technique
Authors: Fusegi Atsushi , Kanao Hiroyuki 
Published: October 2021

AbstractRadical hysterectomy is a standard operation for patients with early-stage cervical cancer. Over the recent decades, laparoscopic radical hysterectomy has been considered an alternative treatment. In 2018, the results of the laparoscopic approach to cervical cancer trial suggested that women with early-stage cervical cancer who underwent minimally invasive surgery for radical hysterectomy had poorer prognosis than those who underwent open surgery. This finding was unexpected, and direct evidence supporting poor prognosis related to minimally invasive radical hysterectomy was not available because the trial was not designed to evaluate the cause of the inferior outcomes. Tumor spillage caused by surgeon-related factors, including squeezing of the uterine cervix and tumor exposure to circulating CO2 gas, is considered to be associated with the poor prognosis of patients who underwent minimally invasive radical hysterectomy. We believe that protective maneuver to avoid tumor spillage is the key to improve oncologic outcomes of cervical cancer. Here, we present a procedure of total laparoscopic nerve-sparing radical hysterectomy for early-stage cervical cancer in which techniques, such as the “no-look no-touch technique,” were used to prevent tumor spillage.
Comprehensive characterization of osseous tissues from impedance measurements by effective medium approximation
Authors: Wei Wenzuo , Shi Fukun , Zhuang Jie , Kolb Juergen F. 
Published: October 2021

A unified mixing (UM) model was developed to derive microstructural information of trabecular bone, i.e., bone volume fraction (BV/TV), from electrical impedance spectroscopy. A distinct advantage of the UM-model over traditional methods, such as equivalent circuit models and multivariate analysis, is that the influence of both the environment (hydroxyapatite) and different inclusions (water, fat, and air) can be taken into account simultaneously. In addition, interactions between the different components such as interfacial polarization can be addressed by a dedicated fitting parameter v. Accordingly, values of BV/TV for different bone samples, e.g., including or not including water, were determined in the higher frequency range of 1–5 MHz. Results showed good agreement with experimental data obtained by micro-computer tomography. In particular, predictions for dielectric parameters that were derived for 3 and 4 MHz were found most promising for the assessment and distinction of osteopathic conditions and differences. This was shown by a clear differentiation of osseous tissues, e.g., the greater trochanter, femoral head, and femoral neck.
Resprouting strategies of three native shrub Cerrado species from a morphoanatomical and chemical perspective
Authors: da Silva Gabriela Santos , Ferraro Alexandre , Lima de Aguiar Claudio , Appezzato-da-Glória Beatriz 
Published: October 2021

This study was carried out in a Cerrado (the largest savanna in the Neotropics) area where pine plantations, introduced in the 1970s, were removed by clear cutting of the trees and burning of the remaining material. After the removal and burning, some native shrub species resprouted. Since resprouting is dependent on buds that can be in the belowground bud-bearing (BBB) organs containing reserves, we selected three resprouting Myrtaceae species for analysis of morphology and anatomy of their BBB organs, to determine which compounds could be accumulated and to investigate the bud protection features. Standard histological techniques were used to analyse the BBB organs. The belowground bud bank at a depth of 10 cm was determined. Nonstructural carbohydrates, total phenolics, and flavonoids were quantified on the roots. The large size of BBB organs suggest that these species were present before plantation establishment and survived plantation management treatments. All species produced a large number of axillary buds. All BBB organs exhibited significant lignification and stored starch and phenolic compounds in the parenchyma cells. The protective features and the storage of reserves associated with the bud-bank allowed the survival and subsequent resprouting of these species, contributing to the regeneration of this disturbed area.
Retinal Microvascular Caliber and Incident Depressive Symptoms: The Multi-Ethnic Study of Atherosclerosis
Authors: van Gennip April C E , Sedaghat Sanaz , Carnethon Mercedes R , Allen Norrina B , Klein Barbara E K , Cotch Mary Frances , Chirinos Diana A , Stehouwer Coen D A , van Sloten Thomas T 
Published: October 2021

Abstract
Cerebral microvascular dysfunction may contribute to depression via disruption of brain structures involved in mood regulation, but evidence is limited. The retina allows for visualization of a microvascular bed that shares similarities with the cerebral microvasculature. We investigated the associations between baseline retinal arteriolar and venular calibers (central retinal arteriolar equivalent (CRAE) and central retinal venular equivalent (CRVE), respectively) and incident depressive symptoms in the Multi-Ethnic Study of Atherosclerosis (MESA). We used longitudinal data on 4,366 participants (mean age = 63.2 years; 48.5% women, 28.4% Black) without baseline depressive symptoms. Depressive symptoms, defined as Center for Epidemiologic Studies Depression Scale score ≥16 and/or use of antidepressant medication, were determined between 2002 and 2004 (baseline; MESA visit 2) and at 3 follow-up examinations conducted every 1.5–2 years thereafter. Fundus photography was performed at baseline. After a mean follow-up period of 6.1 years, 21.9% (n = 958) had incident depressive symptoms. After adjustment for sociodemographic, lifestyle, and cardiovascular factors, a 1–standard-deviation larger baseline CRVE was associated with a higher risk of depressive symptoms (hazard ratio = 1.10, 95% confidence interval: 1.02, 1.17), and a 1–standard-deviation larger baseline CRAE was not statistically significantly associated with incident depressive symptoms (hazard ratio = 1.04, 95% confidence interval: 0.97, 1.11). In this study, larger baseline CRVE, but not CRAE, was associated with a higher incidence of depressive symptoms.
Emotion Word Development in Bilingual Children Living in Majority and Minority Contexts
Authors: Ahn Sunyoung , Chang Charles B 
Published: October 2021

Abstract
The lexicon of emotion words is fundamental to interpersonal communication. To examine how emotion word acquisition interacts with societal context, the present study investigated emotion word development in three groups of child Korean users aged 4–13 years: those who use Korean primarily outside the home as a majority language (MajKCs) or inside the home as a minority language (MinKCs), and those who use Korean both inside and outside the home (KCs). These groups, along with a group of L1 Korean adults, rated the emotional valence of 61 Korean emotion words varying in frequency, valence, and age of acquisition. Results showed KCs, MajKCs, and MinKCs all converging toward adult-like valence ratings by ages 11–13 years; unlike KCs and MajKCs, however, MinKCs did not show age-graded development and continued to diverge from adults in emotion word knowledge by these later ages. These findings support the view that societal context plays a major role in emotion word development, offering one reason for the intergenerational communication difficulties reported by immigrant families.
RNF126 is a positive regulator of TRAF3 ubiquitination
Authors: Kim Soomi , Park Kibeom , Oh Jung-Min , Kim Hongtae 
Published: October 2021

ABSTRACT
Ubiquitination and deubiquitination of signaling molecules are critical regulatory mechanisms in various biological contexts such as inflammatory signaling and the DNA damage response. Thus, finely tuned regulation of protein ubiquitination is essential for maintaining cellular homeostasis. Here, we showed that the RING finger protein RNF126 interacts with TRAF3 and promotes its K63-linked polyubiquitination, which is a crucial step in the TRAF3-dependent antiviral response. We found that RNF126 also interacts with OTUB1, a deubiquitinating enzyme that negatively regulates K63-linked ubiquitination of TRAF3. RNF126 promotes ubiquitination of OTUB1, leading to reduced deubiquitinating activity toward TRAF3. Moreover, RNF126 promotes ubiquitination of OTUB1 on cysteine 91, which is reportedly required for its catalytic activity. Taken together, our results suggest that RNF126 positively regulates the antiviral response by directly promoting K63-linked polyubiquitination of TRAF3 and by reducing OTUB1 activity.
Clustering phosphate and iron-related markers and prognosis in dialysis patients
Authors: Morooka Hikaru , Tanaka Akihito , Inaguma Daijo , Maruyama Shoichi 
Published: October 2021

ABSTRACT

Background
Hyperphosphatemia in patients undergoing dialysis is common and is associated with mortality. Recently, the link between phosphate metabolism and iron dynamics has received increasing attention. However, the association between this relationship and prognosis remains largely unexplored.

Methods
We conducted an observational study of patients who initiated dialysis in the 17 centers participating in the Aichi Cohort Study of the Prognosis in Patients Newly Initiated into Dialysis. Data were available on sex, age, use of phosphate binder, drug history, medical history and laboratory data. After excluding patients with missing values of phosphate, hemoglobin, ferritin and transferrin saturation, we used the Gaussian mixture model to divide the cohort into clusters based on phosphate, hemoglobin, logarithmic ferritin and transferrin saturation. We investigated the prognosis of patients in these clusters. The primary outcome was all-cause death. In each cluster, the prognostic impact of phosphate binder was also studied.

Results
The study included 1175 patients with chronic kidney disease who initiated dialysis between October 2011 and September 2013. Among them, 785 were men and 390 were women, with a mean ± SD age of 67.9 ± 13.0 years. The patients were divided into three clusters, and mortality was higher in cluster c than in cluster a (P = 0.005). Moreover, the use of phosphate binders was associated with a lower risk of all-cause death in two clusters (a and c) that were characterized by older age and higher prevalence of diabetes mellitus, among other things.

Conclusions
We used an unsupervised machine learning method to cluster patients, using phosphate, hemoglobin and iron-related markers. In two of the clusters, the oral use of a phosphate binder might improve prognosis.
Titin (TTN): from molecule to modifications, mechanics, and medical significance
Authors: Loescher Christine M , Hobbach Anastasia J , Linke Wolfgang A 
Published: October 2021

Abstract
The giant sarcomere protein titin is a major determinant of cardiomyocyte stiffness and contributor to cardiac strain sensing. Titin-based forces are highly regulated in health and disease, which aids in the regulation of myocardial function, including cardiac filling and output. Due to the enormous size, complexity, and malleability of the titin molecule, titin properties are also vulnerable to dysregulation, as observed in various cardiac disorders. This review provides an overview of how cardiac titin properties can be changed at a molecular level, including the role isoform diversity and post-translational modifications (acetylation, oxidation, and phosphorylation) play in regulating myocardial stiffness and contractility. We then consider how this regulation becomes unbalanced in heart disease, with an emphasis on changes in titin stiffness and protein quality control. In this context, new insights into the key pathomechanisms of human cardiomyopathy due to a truncation in the titin gene (TTN) are discussed. Along the way, we touch on the potential for titin to be therapeutically targeted to treat acquired or inherited cardiac conditions, such as HFpEF or TTN-truncation cardiomyopathy.
Methodology for the integration of a high-speed train in Maintenance 4.0
Authors: Bustos Alejandro , Rubio Higinio , Soriano-Heras Enrique , Castejon Cristina 
Published: October 2021

AbstractThe fourth industrial revolution is changing the way industries face their problems, including maintenance. The railway industry is moving to adopt this new industry model. The new trains are designed, manufactured, and maintained following an Industry 4.0 methodology, but most of the current trains in operation were not designed with this technological philosophy, so they must be adapted to it. In this paper, a new methodology for adapting a high-speed train to Industry 4.0 is proposed. That way, a train manufactured before this new paradigm can seize the advantages of Maintenance 4.0. This methodology is based on four stages (physical system, digital twin, information and communication technology infrastructure, and diagnosis) that comprise the required processes to digitalize a railway vehicle and that share information between them. The characteristics that the data acquisition and communication systems must fulfil are described, as well as the original signal processing techniques developed for analysing vibration signals. These techniques allow processing experimental data both in real time and deferred, according to actual maintenance requirements. The methodology is applied to determine the operating condition of a high-speed bogie by combining the signal processing of actual vibration measurements taken during the normal train operation and the data obtained from simulations of the digital twin. The combination of both (experimental data and simulations) allows establishing characteristic indicators that correspond to the normal running of the train and indicators that would correspond to anomalies in the behaviour of the train.
Transposon insertion mutation of Antarctic psychrotrophic fungus for red pigment production adaptive to normal temperature
Authors: Ding Lulu , Huang Hezhou , Lu Fengning , Lu Jian , Zhou Xiangshan , Zhang Yuanxing , Cai Menghao 
Published: October 2021

Abstract
Polar regions are rich in microbial and product resources. Geomyces sp. WNF-15A is an Antarctic psy chrotrophic filamentous fungus producing high quality red pigment with potential for industrial use. However, efficient biosynthesis of red pigment can only realize at low temperature, which brings difficult control and high cost for the large-scale fermentation. This study aims to develop transposon insertion mutation method to improve cell growth and red pigment production adaptive to normal temperature. Genetic manipulation system of this fungus was firstly developed by antibiotic marker screening, protoplast preparation and transformation optimization, by which transformation efficiency of ∼50% was finally achieved. Then transposable insertion systems were established using Helitron, Fot1, and Impala transposons. The transposition efficiency reached 11.9%, 9.4%, and 4.6%, respectively. Mutant MP1 achieved the highest red pigment production (OD520 of 39) at 14°C, which was 40% higher than the wild-type strain. Mutant MP14 reached a maximum red pigment production (OD520 of 14.8) at 20°C, which was about twofold of the wild-type strain. Mutants MP2 and MP10 broke the repression mechanism of red pigment biosynthesis in the wild-type and allowed production at 25°C. For cell growth, eight mutants grew remarkably better (12%∼30% biomass higher) than the wild-type at 25°C. This study established an efficient genetic manipulation and transposon insertion mutation platform for polar filamentous fungus. It provides reference for genetic breeding of psychrotrophic fungi from polar and other regions.
Getting on track for digital work: Digital transformation in an administrative court before and during COVID-19
Authors: Björkdahl Joakim , Kronblad Charlotta 
Published: October 2021

AbstractThis article analyses organizational change and new ways of working in one of our most institutionalized and professionalized contexts—the courts. Here, digital technologies and the implementation of digital work practices carry great promise as they enable more accessible and qualitative services to be produced more efficiently and effectively. While prior studies have shown that institutionalized and professionalized actors are reluctant to respond to change, attempts to change work practices through digital technologies remain understudied. In particular, we do not know how COVID-19 has influenced the motivation and implementation of digitalized work. This article draws on a large Swedish administrative court and its attempts to digitalize its work starting in 2018. We find that several barriers first inhibited a successful transformation of work practices. These barriers were connected to the institution of the court and the institutionalized profession of judges, which worked together in preventing organizational change. However, COVID-19 radically accelerated the digital implementation of work practices and gave rise to two separate re-assessment processes. The first established new motivations for digitalized work, and the second allowed for a new perception of value in digital work. These processes effectively broke down perceived barriers and substantially facilitated a more successful digital transformation of working practices.