bluuebunny/crossref_metadata_2025_split huggingface dataset:
Use of paclitaxel carried in lipid nanoparticles to treat aortic allograft transplantation in rats
Authors: Pepineli Rafael , Santana Alexandre C , Silva Filipe M O , Tavoni Thauany M , Stolf Noedir A G , Noronha Irene L , Maranhão Raul C 
Published: April 2021

Abstract

Objectives
The aim of this study was to test whether lipid core nanoparticles loaded with paclitaxel (LDE-PTX) protect rat aortic allograft from immunological damage.

Methods
Fisher and Lewis rats were used differing in minor histocompatibility loci. Sixteen Lewis rats were allocated to four-animal groups: SYNG (syngeneic), Lewis rats receiving aorta grafts from Lewis rats; ALLO (allogeneic), Lewis rats receiving aortas from Fisher rats; ALLO+LDE (allogeneic transplant treated with LDE), Lewis rats receiving aortas from Fisher rats, treated with LDE (weekly injection for 3 weeks); ALLO+LDE-PTX (allogeneic transplant treated with LDE-PTX), Lewis rats receiving aortas from Fisher rats treated with LDE-PTX (4 mg/kg weekly for 3 weeks). Treatments began on transplantation day.

Results
Thirty days post-transplantation, SYNG showed intact aortas. ALLO and ALLO+LDE presented intense neointimal formation. In ALLO+LDE-PTX, treatment inhibited neointimal formation; narrowing of aortic lumen was prevented in ALLO and ALLO+LDE. LDE-PTX strongly inhibited proliferation and intimal invasion by smooth muscle cells, diminished 4-fold presence of apoptotic/dead cells in the intima, reduced the invasion of aorta by macrophages and T-cells and gene expression of pro-inflammatory tumour necrosis factor-alpha (TNFα), interferon gamma (IFNγ) and interleukin-1 beta (IL-1β).

Conclusions
LDE-PTX was effective in preventing the vasculopathy associated with rejection and may offer a potent therapeutic tool for post-transplantation.
Home Away from Home: Examining Adolescent Refugees’ Well-Being in Australia
Authors: Mahadevan Renuka , Jayasinghe Maneka 
Published: April 2021

Abstract
This article examines the correlation of post-migration factors and in particular parental mental health with post-traumatic stress disorder (PTSD) and happiness levels of adolescent refugee migrants in Australia, with a special emphasis on associated age and gender differences. Data were sourced from the child module in the 2015–2016 Building a New Life in Australia data set. Results indicate that factors associated with happiness are quite different from those associated with PTSD and this varies across gender and the age groups of 11–14 and 15–17. For example, discrimination related to religion/culture and language have different correlation with boys’ and girls’ well-being measures. While father’s education and mental state have no significant association with any well-being measure, mothers’ education plays some role. Of concern is the intergenerational correlation of mother’s PTSD with their daughters. The results in this study caution against a one-size-fits-all approach to intervention and suggest that a targeted focus on older and younger adolescents further differentiated by gender is likely to be more effective.
Cyanoacrylate glue for closure of proximal enterocutaneous fistula: a case report
Authors: Alharbi Ahlam Hamed , Alotaibi Abdularahman M 
Published: April 2021

Abstract
Enterocutaneous fistula (ECF) is a distressing complication. Commonly, it follows abdominal operations that require extensive adhesiolysis. Its management is challenging, burdening health systems. Complete healing can take several weeks. Several modalities have been described, with varying success rates. A 48-year-old male underwent a trauma laparotomy, with resection of a segment of the proximal bowel and anastomosis. He experienced an anastomosis leak, wound infection and ECF and was managed conservatively for 5 weeks with parenteral nutrition and bowel rest. He was then referred to us and treated with approximation sutures and cyanoacrylate adhesive. His wound was closed with a subcutaneous drain. He experienced complete healing of the fistula and wound after undergoing a minimally invasive approach using sutures and a cyanoacrylate sealant. Cyanoacrylate glue is a safe initial non-invasive treatment of low-output ECF. It can be selected over approximation sutures to ensure sealing of the tract before surgery.
The properties of polycyclic aromatic hydrocarbons in galaxies: constraints on PAH sizes, charge and radiation fields
Authors: Rigopoulou D , Barale M , Clary D C , Shan X , Alonso-Herrero A , García-Bernete I , Hunt L , Kerkeni B , Pereira-Santaella M , Roche P F 
Published: April 2021

ABSTRACT
Based on theoretical spectra computed using Density Functional Theory we study the properties of polycyclic aromatic hydrocarbons (PAH). In particular using bin-average spectra of PAH molecules with varying number of carbons we investigate how the intensity of the mid-infrared emission bands, 3.3, 6.2, 7.7, and 11.3 $\mu$m, respond to changes in the number of carbons, charge of the molecule, and the hardness of the radiation field that impinges the molecule. We confirm that the 6.2/7.7 band ratio is a good predictor for the size of the PAH molecule (based on the number of carbons present). We also investigate the efficacy of the 11.3/3.3 ratio to trace the size of PAH molecules and note the dependence of this ratio on the hardness of the radiation field. While the ratio can potentially also be used to trace PAH molecular size, a better understanding of the impact of the underlying radiation field on the 3.3 $\mu$m feature and the effect of the extinction on the ratio should be evaluated. The newly developed diagnostics are compared to band ratios measured in a variety of galaxies observed with the Infrared Spectrograph on board the Spitzer Space Telescope. We demonstrate that the band ratios can be used to probe the conditions of the interstellar medium in galaxies and differentiate between environments encountered in normal star forming galaxies and active galactic nuclei. Our work highlights the immense potential that PAH observations with the James Webb Space Telescope will have on our understanding of the PAH emission itself and of the physical conditions in galaxies near and far.
Modelling neutron star–black hole binaries: future pulsar surveys and gravitational wave detectors
Authors: Chattopadhyay Debatri , Stevenson Simon , Hurley Jarrod R , Bailes Matthew , Broekgaarden Floor 
Published: April 2021

ABSTRACT
Binaries comprised of a neutron star (NS) and a black hole (BH) have so far eluded observations as pulsars and with gravitational waves (GWs). We model the formation and evolution of these NS+BH binaries – including pulsar evolution – using the binary population synthesis code COMPAS. We predict the presence of a total of 50–2000 binaries containing a pulsar and a BH (PSR+BHs) in the Galactic field. We find the population observable by the next generation of radio telescopes, represented by the SKA and MeerKAT, current (LIGO/Virgo) and future (LISA) GW detectors. We conclude that the SKA will observe 1–80 PSR+BHs, with 0–4 binaries containing millisecond pulsars. MeerKAT is expected to observe 0–40 PSR+BH systems. Future radio detections of NS+BHs will constrain uncertain binary evolution processes such as BH natal kicks. We show that systems in which the NS formed first (NSBH) can be distinguished from those where the BH formed first (BHNS) by their pulsar and binary properties. We find 40 per cent of the LIGO/Virgo observed NS+BHs from a Milky Way like field population will have a chirp mass ≥3.0 M⊙. We estimate the spin distributions of NS+BHs with two models for the spins of BHs. The remnants of BHNS mergers will have a spin of ∼0.4, whilst NSBH merger remnants can have a spin of ∼0.6 or ∼0.9 depending on the model for BH spins. We estimate that approximately 25–1400 PSR+BHs will be radio alive whilst emitting GWs in the LISA frequency band, raising the possibility of joint observation by the SKA and LISA.
On dust evolution in planet-forming discs in binary systems – I. Theoretical and numerical modelling: radial drift is faster in binary discs
Authors: Zagaria Francesco , Rosotti Giovanni P , Lodato Giuseppe 
Published: April 2021

ABSTRACT
Many stars are in binaries or higher order multiple stellar systems. Although in recent years a large number of binaries have been proven to host exoplanets, how planet formation proceeds in multiple stellar systems has not been studied much yet from the theoretical standpoint. In this paper, we focus on the evolution of the dust grains in planet-forming discs in binaries. We take into account the dynamics of gas and dust in discs around each component of a binary system under the hypothesis that the evolution of the circumprimary and the circumsecondary discs is independent. It is known from previous studies that the secular evolution of the gas in binary discs is hastened due to the tidal interactions with their hosting stars. Here, we prove that binarity affects dust dynamics too, possibly in a more dramatic way than the gas. In particular, the presence of a stellar companion significantly reduces the amount of solids retained in binary discs because of a faster, more efficient radial drift, ultimately shortening their lifetime. We prove that how rapidly discs disperse depends both on the binary separation, with discs in wider binaries living longer, and on the disc viscosity. Although the less-viscous discs lose high amounts of solids in the earliest stages of their evolution, they are dissipated slowly, while those with higher viscosities show an opposite behaviour. The faster radial migration of dust in binary discs has a striking impact on planet formation, which seems to be inhibited in this hostile environment, unless other disc substructures halt radial drift further in. We conclude that if planetesimal formation were viable in binary discs, this process would take place on very short time-scales.
Iblis, Abraham, and Teleological Suspensions
Authors: Hudson Hud 
Published: April 2021

Abstract
In this essay, I shall scold a Jinn, recommend a position in Islamic theology to my Muslim neighbors, explore a famous dilemma recounted in Genesis, and participate in a debate occasioned by an interpretive puzzle in Kierkegaard studies. I investigate two opposed ways of understanding the phrase, ‘the teleological suspension of the ethical’, offer some critical remarks on the interpretation of that phrase in Kierkegaard’s Fear and Trembling, and defend a range of considerations that speak in favor of one of the options over the other. Finally, I show how one may invoke my preferred interpretation to confront the puzzle common to Jewish, Christian, and Islamic theology presented by the story of Abraham and the binding of Isaac and also how a related strategy can be invoked to address the story of Iblis and the puzzle peculiar to Islamic theology to which it gives rise.
Intermolecular dark resonance energy transfer (DRET): upgrading fluorogenic DNA sensing
Authors: Barnoin Guillaume , Shaya Janah , Richert Ludovic , Le Hoang-Ngoan , Vincent Steve , Guérineau Vincent , Mély Yves , Michel Benoît Y , Burger Alain 
Published: April 2021

Abstract
The sensitivity of FRET-based sensing is usually limited by the spectral overlaps of the FRET donor and acceptor, which generate a poor signal-to-noise ratio. To overcome this limitation, a quenched donor presenting a large Stokes shift can be combined with a bright acceptor to perform Dark Resonance Energy Transfer (DRET). The consequent fluorogenic response from the acceptor considerably improves the signal-to-noise ratio. To date, DRET has mainly relied on a donor that is covalently bound to the acceptor. In this context, our aim was to develop the first intermolecular DRET pair for specific sensing of nucleic acid sequences. To this end, we designed DFK, a push–pull probe based on a fluorenyl π-platform that is strongly quenched in water. DFK was incorporated into a series of oligonucleotides and used as a DRET donor with Cy5-labeled complementary sequences. In line with our expectations, excitation of the dark donor in the double-labeled duplex switched on the far-red Cy5 emission and remained free of cross-excitation. The DRET mechanism was supported by time-resolved fluorescence measurements. This concept was then applied with binary probes, which confirmed the distance dependence of DRET as well as its potency in detecting sequences of interest with low background noise.
A nucleotide-sensing endonuclease from the Gabija bacterial defense system
Authors: Cheng Rui , Huang Fengtao , Wu Hui , Lu Xuelin , Yan Yan , Yu Bingbing , Wang Xionglue , Zhu Bin 
Published: April 2021

AbstractThe arms race between bacteria and phages has led to the development of exquisite bacterial defense systems including a number of uncharacterized systems distinct from the well-known restriction-modification and CRISPR/Cas systems. Here, we report functional analyses of the GajA protein from the newly predicted Gabija system. The GajA protein is revealed as a sequence-specific DNA nicking endonuclease unique in that its activity is strictly regulated by nucleotide concentration. NTP and dNTP at physiological concentrations can fully inhibit the robust DNA cleavage activity of GajA. Interestingly, the nucleotide inhibition is mediated by an ATPase-like domain, which usually hydrolyzes ATP to stimulate the DNA cleavage when associated with other nucleases. These features suggest a mechanism of the Gabija defense in which an endonuclease activity is suppressed under normal conditions, while it is activated by the depletion of NTP and dNTP upon the replication and transcription of invading phages. This work highlights a concise strategy to utilize a DNA nicking endonuclease for phage resistance via nucleotide regulation.
DNA polymerase gamma mutations that impair holoenzyme stability cause catalytic subunit depletion
Authors: Silva-Pinheiro Pedro , Pardo-Hernández Carlos , Reyes Aurelio , Tilokani Lisa , Mishra Anup , Cerutti Raffaele , Li Shuaifeng , Rozsivalova Dieu-Hien , Valenzuela Sebastian , Dogan Sukru A , Peter Bradley , Fernández-Silva Patricio , Trifunovic Aleksandra , Prudent Julien , Minczuk Michal , Bindoff Laurence , Macao Bertil , Zeviani Massimo , Falkenberg Maria , Viscomi Carlo 
Published: April 2021

AbstractMutations in POLG, encoding POLγA, the catalytic subunit of the mitochondrial DNA polymerase, cause a spectrum of disorders characterized by mtDNA instability. However, the molecular pathogenesis of POLG-related diseases is poorly understood and efficient treatments are missing. Here, we generate the PolgA449T/A449T mouse model, which reproduces the A467T change, the most common human recessive mutation of POLG. We show that the mouse A449T mutation impairs DNA binding and mtDNA synthesis activities of POLγ, leading to a stalling phenotype. Most importantly, the A449T mutation also strongly impairs interactions with POLγB, the accessory subunit of the POLγ holoenzyme. This allows the free POLγA to become a substrate for LONP1 protease degradation, leading to dramatically reduced levels of POLγA in A449T mouse tissues. Therefore, in addition to its role as a processivity factor, POLγB acts to stabilize POLγA and to prevent LONP1-dependent degradation. Notably, we validated this mechanism for other disease-associated mutations affecting the interaction between the two POLγ subunits. We suggest that targeting POLγA turnover can be exploited as a target for the development of future therapies.
Development and Validation of a Multivariable Prediction Model for Missed HIV Health Care Provider Visits in a Large US Clinical Cohort
Authors: Pettit April C , Bian Aihua , Schember Cassandra O , Rebeiro Peter F , Keruly Jeanne C , Mayer Kenneth H , Mathews W Christopher , Moore Richard D , Crane Heidi M , Geng Elvin , Napravnik Sonia , Shepherd Bryan E , Mugavero Michael J 
Published: April 2021

Abstract

Background
Identifying individuals at high risk of missing HIV care provider visits could support proactive intervention. Previous prediction models for missed visits have not incorporated data beyond the individual level.

Methods
We developed prediction models for missed visits among people with HIV (PWH) with ≥1 follow-up visit in the Center for AIDS Research Network of Integrated Clinical Systems from 2010 to 2016. Individual-level (medical record data and patient-reported outcomes), community-level (American Community Survey), HIV care site–level (standardized clinic leadership survey), and structural-level (HIV criminalization laws, Medicaid expansion, and state AIDS Drug Assistance Program budget) predictors were included. Models were developed using random forests with 10-fold cross-validation; candidate models with the highest area under the curve (AUC) were identified.

Results
Data from 382 432 visits among 20 807 PWH followed for a median of 3.8 years were included; the median age was 44 years, 81% were male, 37% were Black, 15% reported injection drug use, and 57% reported male-to-male sexual contact. The highest AUC was 0.76, and the strongest predictors were at the individual level (prior visit adherence, age, CD4+ count) and community level (proportion living in poverty, unemployed, and of Black race). A simplified model, including readily accessible variables available in a web-based calculator, had a slightly lower AUC of .700.

Conclusions
Prediction models validated using multilevel data had a similar AUC to previous models developed using only individual-level data. The strongest predictors were individual-level variables, particularly prior visit adherence, though community-level variables were also predictive. Absent additional data, PWH with previous missed visits should be prioritized by interventions to improve visit adherence.
Diabetic ketoacidosis at presentation of type 1 diabetes in children in Canada during the COVID-19 pandemic
Authors: Sellers Elizabeth A C , Pacaud Danièle 
Published: April 2021

Abstract
Type 1 diabetes is a common chronic illness in childhood. Diabetic ketoacidosis (DKA) is the leading cause of morbidity and mortality in children with type 1 diabetes. Early recognition of symptoms of diabetes and immediate initiation of treatment are important factors in preventing DKA at first presentation. We describe the numbers of children presenting with DKA at initial diagnosis across eight Canadian paediatric centres during the COVID-19 pandemic (March 15, 2020 to July 31, 2020) and compare this to the same time period in 2019. Comparing the pre-COVID to the COVID-19 time period, presentation in DKA increased from 36.4% to 55.0% (P&lt;0.0001) and presentation in severe DKA from 37.0% to 48.3% (P=0.044). These findings are concerning and emphasize the importance of awareness of the signs and symptoms of diabetes. In addition, these findings raise concern about access to appropriate and timely care during the COVID-19 pandemic.
Making the ‘Green Revolution’ Truly Green: Improving Crop Nitrogen Use Efficiency
Authors: Wang Fanmiao , Yoshida Hideki , Matsuoka Makoto 
Published: April 2021

Abstract
Traditional breeding for high-yielding crops has mainly relied on the widespread cultivation of gibberellin (GA)-deficient semi-dwarf varieties, as dwarfism increases lodging resistance and allows for high nitrogen use, resulting in high grain yield. Although the adoption of semi-dwarf varieties in rice and wheat breeding brought big success to the ‘Green Revolution’ in the 20th century, it consequently increased the demand for nitrogen-based fertilizer, which causes severe threat to ecosystems and sustainable agriculture. To make the ‘Green Revolution’ truly green, it is necessary to develop new varieties with high nitrogen use efficiency (NUE). Under this demand, research on NUE, mainly for rice, has made great strides in the last decade. This mini-review focuses on three aspects of recent epoch-making findings on rice breeding for high NUE. The first one on ‘NUE genes related to GA signaling’ shows how promising it is to improve NUE in semi-dwarf Green Revolution varieties. The second aspect centers around the nitrate transporter1.1B, NRT1.1B; studies have revealed a nutrient signaling pathway through the discovery of the nitrate-NRT1.1B-SPX4-NLP3 cascade. The last one is based on the recent finding that the teosinte branched1, cycloidea, proliferating cell factor (TCP)-domain protein 19 underlies the genomic basis of geographical adaptation to soil nitrogen; OsTCP19 regulates the expression of a key transacting factor, DLT/SMOS2, which participates in the signaling of four different phytohormones, GA, auxin, brassinosteroid and strigolactone. Collectively, these breakthrough findings represent a significant step toward breeding high-NUE rice in the future.
The Unicellular Red AlgaCyanidioschyzon merolae—The Simplest Model of a Photosynthetic Eukaryote
Authors: Miyagishima Shin-Ya , Tanaka Kan 
Published: April 2021

AbstractSeveral species of unicellular eukaryotic algae exhibit relatively simple genomic and cellular architecture. Laboratory cultures of these algae grow faster than plants and often provide homogeneous cellular populations exposed to an almost equal environment. These characteristics are ideal for conducting experiments at the cellular and subcellular levels. Many microalgal lineages have recently become genetically tractable, which have started to evoke new streams of studies. Among such algae, the unicellular red alga Cyanidioschyzon merolae is the simplest organism; it possesses the minimum number of membranous organelles, only 4,775 protein-coding genes in the nucleus, and its cell cycle progression can be highly synchronized with the diel cycle. These properties facilitate diverse omics analyses of cellular proliferation and structural analyses of the intracellular relationship among organelles. C. merolae cells lack a rigid cell wall and are thus relatively easily disrupted, facilitating biochemical analyses. Multiple chromosomal loci can be edited by highly efficient homologous recombination. The procedures for the inducible/repressive expression of a transgene or an endogenous gene in the nucleus and for chloroplast genome modification have also been developed. Here, we summarize the features and experimental techniques of C. merolae and provide examples of studies using this alga. From these studies, it is clear that C. merolae—either alone or in comparative and combinatory studies with other photosynthetic organisms—can provide significant insights into the biology of photosynthetic eukaryotes.