bluuebunny/crossref_metadata_2025_split huggingface dataset:
Molecular identification of Pentatrichomonas hominis in animals in central and western Thailand
Authors: Mahittikorn Aongart , Udonsom Ruenruetai , Koompapong Khuanchai , Chiabchalard Rachatawan , Sutthikornchai Chantira , Sreepian Preeyaporn Monatrakul , Mori Hirotake , Popruk Supaluk 
Published: June 2021

Abstract
Background
Pentatrichomonas hominis inhabits the digestive tracts of several vertebrates, such as humans, monkeys, pigs, dogs, cats and rats. This protozoan was originally considered a commensal of the digestive tract but has subsequently been identified as a potential zoonotic parasite and a causative agent of diarrhoea. Molecular techniques are considered more sensitive and specific to detect P. hominis. This study aimed to determine the presence and genetic diversity of P. hominis in animals in Thailand. A total of 403 faecal samples were collected from 119 cats, 55 dogs, 73 goats, 35 monkeys, 55 cattle and 66 pigs, and the presence of P. hominis was determined using the nested polymerase chain reaction method. Sequence analysis of small-subunit ribosomal RNA genes was used to determine the genotype of the organism.

Results
Twenty-six samples (26/403, 6.45%) were positive for P. hominis. The highest prevalence was found in cats (21/119; 17.65%), followed by cattle (3/55; 5.45%) and dogs (2/55; 3.64%). Seven out of 26 nucleotides demonstrated 100% sequence identity with existing sequences; additionally, 16 novel sequence patterns were identified. All nucleotide sequences of P. hominis-positive samples were shown in the same branch with the previously described P. hominis sequences found in humans, dogs and goat.

Conclusion
This is the first study on P. hominis infections in animals in Thailand. Our findings revealed that the prevalence of P. hominis was significantly higher in cats than in cattle and dogs. Cats were the main reservoir host; however, P. hominis can infect several kinds of animals. Therefore, the proper waste management of animals is necessary to reduce and prevent infection in the community.
The effect of dietary supplementation with silkworm pupae meal on gastrointestinal function, nitrogen retention and blood biochemical parameters in rabbits
Authors: Gugołek Andrzej , Kowalska Dorota , Strychalski Janusz , Ognik Katarzyna , Juśkiewicz Jerzy 
Published: June 2021

Abstract
Background
The aim of this study was to determine the effect of dietary inclusion of silkworm pupae meal (SPM) on nutrient digestibility, nitrogen utilization, gastrointestinal physiology and blood biochemical parameters in rabbits. Thirty Termond White rabbits were divided into three groups: SBM – fed a diet containing 10% soybean meal (SBM), SPM5 – fed a diet containing 5% SBM and 5% SPM, and SPM10 – fed a diet containing 10% SPM.

Results
Nutrient digestibility and nitrogen retention decreased with increasing SPM inclusion levels in rabbit diets. The dietary inclusion of SPM caused a significant increase in the stomach pH. Group SPM10 rabbits were characterized by the highest cecal tissue and digesta weights. The lowest cecal pH was noted in group SPM5. The relative weights of colonic tissue and digesta tended to increase with increasing levels of SPM. The total and intracellular activity of bacterial α-galactosidase decreased significantly in both SPM groups. The replacement of SBM with SPM led to a decrease in the activity of bacterial β-glucuronidase in the cecal digesta. The intracellular activity of bacterial α-arabinofuranosidase increased, and its release rate decreased in the cecum of rabbits in SPM groups. The extracellular activity of bacterial β-xylosidase in the cecal digesta tended to decrease in group SPM10. The highest extracellular and intracellular activity of bacterial β-cellobiosidase in the cecal digesta was noted in the SPM5 treatment. The lowest and the highest activity of bacterial N-acetyl-β-D-glucosaminidase (NAGase) was observed in groups SBM and SPM10. The SPM10 treatment contributed to a decrease in the cecal concentrations of butyric, iso-valeric and valeric acids. The lowest total concentration of putrefactive short-chain fatty acids (PSCFAs) was observed in group SPM10. The cecal concentration of propionic acid tended to increase in group SPM5, whereas the cecal concentration of iso-butyric acid tended to decrease in group SPM10. The colonic concentration of iso-valeric acid was lowest in group SPM5. SPM treatments resulted in a significant increase in plasma albumin concentration. Plasma urea concentration was significantly higher in group SPM10 than in SBM and SPM5.

Conclusions
The results of this study suggest that rabbit diets can be supplemented with SPM at up to 5%.
Identification of prognostic lipid droplet-associated genes in pancreatic cancer patients via bioinformatics analysis
Authors: Bai Rubing , Rebelo Artur , Kleeff Jörg , Sunami Yoshiaki 
Published: June 2021

Abstract
Background
Pancreatic cancer is the fourth leading cause of cancer deaths in the United States both in females and in males, and is projected to become the second deadliest cancer by 2030. The overall 5-year survival rate remains at around 10%. Cancer metabolism and specifically lipid metabolism plays an important role in pancreatic cancer progression and metastasis. Lipid droplets can not only store and transfer lipids, but also act as molecular messengers, and signaling factors. As lipid droplets are implicated in reprogramming tumor cell metabolism and in invasion and migration of pancreatic cancer cells, we aimed to identify lipid droplet-associated genes as prognostic markers in pancreatic cancer.

Methods
We performed a literature search on review articles related to lipid droplet-associated proteins. To select relevant lipid droplet-associated factors, bioinformatics analysis on the GEPIA platform (data are publicly available) was carried out for selected genes to identify differential expression in pancreatic cancer versus healthy pancreatic tissues. Differentially expressed genes were further analyzed regarding overall survival of pancreatic cancer patients.

Results
65 factors were identified as lipid droplet-associated factors. Bioinformatics analysis of 179 pancreatic cancer samples and 171 normal pancreatic tissue samples on the GEPIA platform identified 39 deferentially expressed genes in pancreatic cancer with 36 up-regulated genes (ACSL3, ACSL4, AGPAT2, BSCL2, CAV1, CAV2, CAVIN1, CES1, CIDEC, DGAT1, DGAT2, FAF2, G0S2, HILPDA, HSD17B11, ICE2, LDAH, LIPE, LPCAT1, LPCAT2, LPIN1, MGLL, NAPA, NCEH1, PCYT1A, PLIN2, PLIN3, RAB5A, RAB7A, RAB8A, RAB18, SNAP23, SQLE, VAPA, VCP, VMP1) and 3 down-regulated genes (FITM1, PLIN4, PLIN5). Among 39 differentially expressed factors, seven up-regulated genes (CAV2, CIDEC, HILPDA, HSD17B11, NCEH1, RAB5A, and SQLE) and two down-regulation genes (BSCL2 and FITM1) were significantly associated with overall survival of pancreatic cancer patients. Multivariate Cox regression analysis identified CAV2 as the only independent prognostic factor.

Conclusions
Through bioinformatics analysis, we identified nine prognostic relevant differentially expressed genes highlighting the role of lipid droplet-associated factors in pancreatic cancer.
The occurrence of coronary artery lesions in Kawasaki disease based on C-reactive protein levels: a retrospective cohort study
Authors: An Hyo Soon , Kim Gi Beom , Song Mi Kyoung , Lee Sang Yun , Kwon Hye Won , Lee Joo Won , Bae Eun Jung 
Published: June 2021

Abstract
Background
This study aimed to assess the occurrence of coronary artery lesions (CAL) in patients with Kawasaki disease (KD) according to serum C-reactive protein (CRP) levels.

Methods
This retrospective analysis was based on the nationwide survey of KD conducted in the Republic of Korea between 2015 and 2017. We enrolled 9131 patients and defined low (< 3 mg/dL) and high (≥3 mg/dL) CRP groups. Demographic data, clinical characteristics, z-scores, and scores based on the Japanese criteria for CAL were compared between the two groups. Logistic regression analysis was used to identify CAL risk factors.

Results
The low CRP group accounted for 23% of patients. The mean age at diagnosis was higher in high CRP group compared to the low CRP group (34.4 ± 24.9 vs 31.7 ± 24.8 months, p < 0.001). Fever duration before treatment was not significantly different between the two groups (5.1 ± 1.7 days vs. 5.2 ± 2.1 days; p = 0.206). A non-response to intravenous immunoglobulin treatment was found in 1377 patients (20.1%) and 225 patients (11.7%) in the high and low CRP groups, respectively (p < 0.001). CAL were found in 12.9 and 18.3% of the high and low CRP patients, respectively (p < 0.001), based on z-scores; and in 9.9 and 12.5%, respectively (p = 0.001), based on the Japanese criteria in the acute phase. The giant coronary artery aneurysm occurrence ratio was similar between groups (p = 1.0).

Conclusions
CAL occurred in patients with both high and low CRP. Therefore, patients with KD should be carefully monitored regardless of their CRP levels.
Notes on current Mbyá-Guarani medicinal plant exchanges in southern Brazil
Authors: de Andrade Julian Henrique Carlotto , Rodrigues José , Benites André , Benites Cornélio , Acosta Arlindo , Benites Marcelina , Benites Cocelina , Gomes Ilda , da Silva Jaime Valdir , Antunes Eunice , Antunes Elisete , Martins José , Timóteo Daniel Martins , Franco Santiago , Morinico José Cirilo Pires , da Silva Fernanda Ribeiro , Hanazaki Natalia 
Published: June 2021

AbstractBackgroundExperts in the Atlantic Forest, the Guarani people have the habit of transporting and exchanging plants due to their mobility throughout the territory. Historically, this habit contributed to the species composition and diversification among different phytophysiognomies that comprise the Atlantic Forest. Medicine and spirituality are traits that stand out within the Guarani culture, which is based on a holistic understanding of physical and spiritual well-being for the person’s health. To achieve this balance, they use a range of native and adapted plant species. Our goal is to understand some of the Guarani contributions to the cultural landscape in the Atlantic Forest.MethodsWe conducted semi-structured interviews with key persons asking about the importance of forest environments for Guarani health and about visits to other Guarani villages and plants exchanged. Data analysis was qualitative, and through a bipartite network of exchanged plants to show current plant exchanges between villages.ResultsWe visited seven Guarani Indigenous Territories in south Brazil, and with the participation of 12 respondents, we registered 27 species that were exchanged through different phytophysiognomies in the Atlantic Forest. These results show an intense movement of plants currently occurring between villages and the importance of these movements for both individual health and the integrity of the environments in which the Guarani villages are inserted.ConclusionsWe observed a search for the maintenance of traditional species in the Guarani medical system, and we highlight the fundamental role of Guarani management in the conservation of the southern Atlantic Forest in indigenous territories.
CT and MRI radiomics of bone and soft-tissue sarcomas: a systematic review of reproducibility and validation strategies
Authors: Gitto Salvatore , Cuocolo Renato , Albano Domenico , Morelli Francesco , Pescatori Lorenzo Carlo , Messina Carmelo , Imbriaco Massimo , Sconfienza Luca Maria 
Published: June 2021

Abstract
Background
Feature reproducibility and model validation are two main challenges of radiomics. This study aims to systematically review radiomic feature reproducibility and predictive model validation strategies in studies dealing with CT and MRI radiomics of bone and soft-tissue sarcomas. The ultimate goal is to promote achieving a consensus on these aspects in radiomic workflows and facilitate clinical transferability.

Results
Out of 278 identified papers, forty-nine papers published between 2008 and 2020 were included. They dealt with radiomics of bone (n = 12) or soft-tissue (n = 37) tumors. Eighteen (37%) studies included a feature reproducibility analysis. Inter-/intra-reader segmentation variability was the theme of reproducibility analysis in 16 (33%) investigations, outnumbering the analyses focused on image acquisition or post-processing (n = 2, 4%). The intraclass correlation coefficient was the most commonly used statistical method to assess reproducibility, which ranged from 0.6 and 0.9. At least one machine learning validation technique was used for model development in 25 (51%) papers, and K-fold cross-validation was the most commonly employed. A clinical validation of the model was reported in 19 (39%) papers. It was performed using a separate dataset from the primary institution (i.e., internal validation) in 14 (29%) studies and an independent dataset related to different scanners or from another institution (i.e., independent validation) in 5 (10%) studies.

Conclusions
The issues of radiomic feature reproducibility and model validation varied largely among the studies dealing with musculoskeletal sarcomas and should be addressed in future investigations to bring the field of radiomics from a preclinical research area to the clinical stage.
Human amnion-derived mesenchymal stem cells improved the reproductive function of age-related diminished ovarian reserve in mice through Ampk/FoxO3a signaling pathway
Authors: Liu Hanwen , Jiang Chunyan , La Boya , Cao Meng , Ning Song , Zhou Jing , Yan Zhengjie , Li Chuyu , Cui Yugui , Ma Xiang , Wang Meilian , Chen Li , Yu Youjia , Chen Feng , Zhang Yuexin , Wu Huimin , Liu Jiayin , Qin Lianju 
Published: June 2021

Abstract
Background
Age-related diminished ovarian reserve (AR-DOR) reduced the quality of oocytes, resulting in decreased female fertility. Aging is tightly related to abnormal distribution and function of mitochondria, while mitophagy is a major process to maintain normal quality and quantity of mitochondria in cells, especially in oocytes which containing a large number of mitochondria to meet the demand of energy production during oocyte maturation and subsequent embryonic development. Ampk/FoxO3a signaling is crucial in the regulation of mitophagy. It is reported mesenchymal stem cells (MSCs) can improve ovarian function. Here we aim to explore if human amnion-derived mesenchymal stem cells (hAMSCs) are effective in improving ovarian function in AR-DOR mice and whether Ampk/FoxO3a signaling is involved.

Methods
The AR-DOR model mice were established by 32-week-old mice with 3–8 litters, significantly low serum sex hormone levels and follicle counts. The old mice were divided into 5 treatment groups: normal saline (NS, control), 1% human serum albumin (HSA, resolver), low dose (LD, 5.0 × 106cells/kg), middle dose (MD, 7.5 × 106cells/kg), and high dose (HD, 10.0 × 106cells/kg). The prepared hAMSCs were injected through tail vein. Serum sex hormone level, follicle counts, fertilization rate, gestation rate, little size, apoptosis of granulosa and stromal cells, expression level of Sod2, Ampk, and ratio of phosphorylated FoxO3a to total FoxO3a in ovaries were examined.

Results
Our results show that after hAMSC transplantation, the ovarian function in AR-DOR mice was significantly improved, meanwhile the apoptosis of granulosa and stromal cells in the ovaries was significantly repressed, the expression level of Ampk and the ratio of phosphorylated FoxO3a to total FoxO3a both were significantly increased, meanwhile increased Sod2 expression was also observed.

Conclusion
Our results demonstrate hAMSC transplantation via tail-injection can improve ovarian function of AR-DOR mice through Ampk/FoxO3a signaling pathway.
Epidural catheter migration during cesarean
Authors: AlJa’bari Aboud 
Published: June 2021

Abstract
Background
Early detection and vigilance of high spinal anesthesia post epidural catheter migration in cesarean section leads to safe conduct of anesthesia. Our case describes the migration of a previously functioning epidural catheter in the subarachnoid space.
This migration can be explained by patient posture changes and movements.

Case presentation
A 32 year – old G2P0 medically free female parturient (height 160cm, weight 65 kg), admitted to the labor ward with a 4 cm cervical dilatation, an epidural catheter was inserted in the L3-4 space, and an aspiration test was negative for CSF/blood through epidural catheter.
Epidural catheter was fixed on her back using sterile dressings. Epidural mixture of 0.1% bupivacaine and fentanyl 2 mcg/ml started. Due to fetal distress, cesarean section was urgently planned. She was given a bolus dose through the epidural catheter,10 minutes after skin incision, the patient suddenly started to complain of difficulty of breathing and drowsiness. Moreover, her oxygen saturation suddenly started to drop so rapid sequence induction with cricoid pressure applied and was performed till she was intubated. Her pupils were reactive and dilated. She had stable vital signs. She was reversed with neostigmine and atropine after the use of nerve stimulator.
Aspiration from the epidural catheter was performed. A clear 10mls fluid was aspirated. The fluid was sent to the lab for analysis and found to be CSF. Upon extubation, the patient was conscious and obeying commands. She completely recovered the motor power of her upper and lower limbs while she was admitted to ICU for observation and she was discharged the next day without any residual anesthesia.

Conclusion
Aspiration test and epinephrine test dose is always recommend to be performed prior to local epidural anesthetic for cesarean section even if the function of the epidural catheter was previously established. Careful observation of neurologic signs is also important.
Potent phytochemicals against COVID-19 infection from phyto-materials used as antivirals in complementary medicines: a review
Authors: Sharanya C. S. , Sabu A. , Haridas M. 
Published: June 2021

Abstract
Background
Following the outbreak of the COVID-19 pandemic, there was a surge of research activity to find methods/drugs to treat it. There has been drug-repurposing research focusing on traditional medicines. Concomitantly, many researchers tried to find in silico evidence for traditional medicines. There is a great increase in article publication to commensurate the new-found research interests. This situation inspired the authors to have a comprehensive understanding of the multitude of publications related to the COVID-19 pandemic with a wish to get promising drug leads.

Main body
This review article has been conceived and made as a hybrid of the review of the selected papers advertised recently and produced in the interest of the COVID-19 situation, and in silico work done by the authors. The outcome of the present review underscores a recommendation for thorough MDS analyses of the promising drug leads. The inclusion of in silico work as an addition to the review was motivated by a recently published article of Toelzer and colleagues. The in silico investigation of free fatty acids is novel to the field and it buttresses the further MDS analysis of drug leads for managing the COVID-19 pandemic.

Conclusion
The review performed threw light on the need for MDS analyses to be considered together with the application of other in silico methods of prediction of pharmacologic properties directing towards the sites of drug-receptor regulation. Also, the present analysis would help formulate new recipes for complementary medicines.
Seismic cracking mechanism and control for pre-stressed concrete box girders of continuous rigid-frame bridges: Miaoziping bridge in Wenchuan earthquake as an example
Authors: Tong Lei , Wang Rongxia , Wang Dongsheng 
Published: June 2021

AbstractThe box girder of the Miaoziping Bridge, a three-span prestressed concrete continuous rigid-frame bridge, suffered a serious crack in its box section’s web near the 1/6 to 1/2 length of the side span and the middle-span length of 1/4 to 3/4, as a result of the 2008 Wenchuan earthquake, which also caused large lateral residual displacements at both ends of the side span. In this study, eight strong-motion records near the bridge site and two other records (El Centro and Taft) are selected as inputs for time-history analysis of the bridge. The cantilever construction process and initial stress of the box girder are considered in a bridge model for seismic numerical simulation. Further, the simulation results are compared with the actual earthquake damage. The cracking mechanism, influencing factors and control of the girder crack damage are discussed. The high-stress zones of the box girder agree with the seismic damage observed, even various seismic inputs are considered. The findings reveal that the maximum (principal) tensile stress of the girder exceeds the tensile strength of the concrete under the seismic excitations, and cracks occur. Under various input directions of ground motions, the proportion of the main girder stresses induced by the earthquake shows differences. After the failure of the shear keys in the transverse direction of the bridge, the stresses of the girder decrease in the mid-span. However, the beams at both ends of the side spans revealed large lateral displacements. Considering that the uplift of the beam ends, stress and axial torque of the girder’s side span are greatly reduced. Setting bi-directional friction pendulum bearings on the transition pier is an effective damping measure to control web cracking of the mid-span and lateral drifts of the beam ends.