bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effects of Transcranial Direct Current Stimulation on effort during a working-memory task
Authors: Framorando David , Cai Tianlan , Wang Yi , Pegna Alan J. 
Published: August 2021

AbstractTranscranial Direct Current Stimulation (tDCS) has shown that stimulation of Dorsolateral Prefrontal Cortex (DLPFC) facilitates task performance in working-memory tasks. However, little is known about its potential effects on effort. This study examined whether tDCS affects effort during a working-memory task. Participants received anodal, cathodal and sham stimulation over DLPFC across three sessions before carrying out a 2-back task. During the task, effort-related cardiovascular measures were recorded—especially the Initial Systolic Time Interval (ISTI). Results showed that anodal stimulation produced a shorter ISTI, indicating a greater effort compared to cathodal and sham conditions, where effort was lower. These findings demonstrate that anodal stimulation helps participants to maintain engagement in a highly demanding task (by increasing task mastery), without which they would otherwise disengage. This study is the first to show that tDCS impacts the extent of effort engaged by individuals during a difficult task.
Comparing the COVID-19 pandemic in space and over time in Europe, using numbers of deaths, crude rates and adjusted mortality trend ratios
Authors: Gallo Valentina , Chiodini Paolo , Bruzzese Dario , Kondilis Elias , Howdon Dan , Mierau Jochen , Bhopal Raj 
Published: August 2021

AbstractComparison of COVID-19 trends in space and over time is essential to monitor the pandemic and to indirectly evaluate non-pharmacological policies aimed at reducing the burden of disease. Given the specific age- and sex- distribution of COVID-19 mortality, the underlying sex- and age-distribution of populations need to be accounted for. The aim of this paper is to present a method for monitoring trends of COVID-19 using adjusted mortality trend ratios (AMTRs). Age- and sex-mortality distribution of a reference European population (N = 14,086) was used to calculate age- and sex-specific mortality rates. These were applied to each country to calculate the expected deaths. Adjusted Mortality Trend Ratios (AMTRs) with 95% confidence intervals (C.I.) were calculated for selected European countries on a daily basis from 17th March 2020 to 29th April 2021 by dividing observed cumulative mortality, by expected mortality, times the crude mortality of the reference population. These estimated the sex- and age-adjusted mortality for COVID-19 per million population in each country. United Kingdom experienced the highest number of COVID-19 related death in Europe. Crude mortality rates were highest Hungary, Czech Republic, and Luxembourg. Accounting for the age-and sex-distribution of the underlying populations with AMTRs for each European country, four different patterns were identified: countries which experienced a two-wave pandemic, countries with almost undetectable first wave, but with either a fast or a slow increase of mortality during the second wave; countries with consistently low rates throughout the period. AMTRs were highest in Eastern European countries (Hungary, Czech Republic, Slovakia, and Poland). Our methods allow a fair comparison of mortality in space and over time. These might be of use to indirectly estimating the efficacy of non-pharmacological health policies. The authors urge the World Health Organisation, given the absence of age and sex-specific mortality data for direct standardisation, to adopt this method to estimate the comparative mortality from COVID-19 pandemic worldwide.
Dietary intake of tocopherols and risk of incident disabling dementia
Authors: Aoki Shoko , Yamagishi Kazumasa , Maruyama Koutatsu , Kishida Rie , Ikeda Ai , Umesawa Mitsumasa , Renzhe Cui , Kubota Yasuhiko , Hayama-Terada Mina , Shimizu Yuji , Muraki Isao , Imano Hironori , Sankai Tomoko , Okada Takeo , Kitamura Akihiko , Kiyama Masahiko , Iso Hiroyasu 
Published: August 2021

AbstractTocopherols, strong antioxidants, may be useful in preventing dementia, but the epidemiological evidence is insufficient. We performed a community-based follow-up study of Japanese, the Circulatory Risk in Community Study, involving 3739 people aged 40–64 years at baseline (1985–1999). Incident disabling dementia was followed up from 1999 through 2020. For subtype analysis, we classified disabling dementia into that with and that without a history of stroke. Dietary intake of tocopherols (total, α, β, γ, and δ) were estimated using 24-h recall surveys. During a median follow-up of 19.7 years, 670 cases of disabling dementia developed. Total tocopherol intake was inversely associated with risk of disabling dementia with multivariable hazard ratios (95% confidence intervals) of 0.79 (0.63–1.00) for the highest versus lowest quartiles of total tocopherol intake (P for trend = 0.05). However, the association was strengthened when further adjusted for α-linolenic acid intake (Spearman correlation with total tocopherol intake = 0.93), with multivariable hazard ratios of 0.50 (0.34–0.74) (P for trend = 0.001) but was weakened and nonsignificant when further adjusted for linoleic acid intake (Spearman correlation with total tocopherol intake = 0.92), with multivariable hazard ratios of 0.69 (0.47–1.01) (P for trend = 0.05). Similar but nonsignificant inverse associations were observed for α-, γ-, and δ-tocopherols but not for β-tocopherol. These results were similar regardless of the presence of a history of stroke. Dietary tocopherol intake was inversely associated with risk of disabling dementia, but its independent effect was uncertain owing to a high intercorrelation of α-linolenic linoleic acids with total tocopherol intake. Even with such confounding, a diet high in tocopherols may help prevent the onset of dementia.
NOTUM promotes thermogenic capacity and protects against diet-induced obesity in male mice
Authors: Guo Fangfei , Seldin Marcus , Péterfy Miklós , Charugundla Sarada , Zhou Zhiqiang , Lee Stephen D. , Mouton Alice , Rajbhandari Prashant , Zhang Wenchao , Pellegrini Matteo , Tontonoz Peter , Lusis Aldons J. , Shih Diana M. 
Published: August 2021

AbstractWe recently showed that NOTUM, a liver-secreted Wnt inhibitor, can acutely promote browning of white adipose. We now report studies of chronic overexpression of NOTUM in liver indicating that it protects against diet-induced obesity and improves glucose homeostasis in mice. Adeno-associated virus (AAV) vectors were used to overexpress GFP or mouse Notum in the livers of male C57BL/6J mice and the mice were fed an obesifying diet. After 14 weeks of high fat, high sucrose diet feeding, the AAV-Notum mice exhibited decreased obesity and improved glucose tolerance compared to the AAV-GFP mice. Gene expression and immunoblotting analysis of the inguinal fat and brown fat revealed increased expression of beige/brown adipocyte markers in the AAV-Notum group, suggesting enhanced thermogenic capacity by NOTUM. A β3 adrenergic receptor agonist-stimulated lipolysis test suggested increased lipolysis capacity by NOTUM. The levels of collagen and C–C motif chemokine ligand 2 (CCL2) in the epididymal white adipose tissue of the AAV-Notum mice were significantly reduced, suggesting decreased fibrosis and inflammation, respectively. RNA sequencing analysis of inguinal white adipose of 4-week chow diet-fed mice revealed a highly significant enrichment of extracellular matrix (ECM) functional cluster among the down-regulated genes in the AAV-Notum group, suggesting a potential mechanism contributing to improved glucose homeostasis. Our in vitro studies demonstrated that recombinant human NOTUM protein blocked the inhibitory effects of WNT3A on brown adipocyte differentiation. Furthermore, NOTUM attenuated WNT3A’s effects on upregulation of TGF-β signaling and its downstream targets. Overall, our data suggest that NOTUM modulates adipose tissue function by promoting thermogenic capacity and inhibiting fibrosis through inhibition of Wnt signaling.
Low-energy electron beam has severe impact on seedling development compared to cold atmospheric pressure plasma
Authors: Waskow A. , Butscher D. , Oberbossel G. , Klöti D. , Rudolf von Rohr P. , Büttner-Mainik A. , Drissner D. , Schuppler M. 
Published: August 2021

AbstractSprouts are germinated seeds that are often consumed due to their high nutritional content and health benefits. However, the conditions for germination strongly support the proliferation of present bacteria, including foodborne pathogens. Since sprouts are consumed raw or minimally processed, they are frequently linked to cases of food poisoning. Therefore, a seed decontamination method that provides efficient inactivation of microbial pathogens, while maintaining the germination capacity and quality of the seeds is in high demand. This study aimed to investigate and compare seed decontamination by cold atmospheric-pressure plasma and low-energy electron beam with respect to their impact on seed and seedling quality. The results show that both technologies provide great potential for inactivation of microorganisms on seeds, while cold plasma yielded a higher efficiency with 5 log units compared to a maximum of 3 log units after electron beam treatment. Both techniques accelerated seed germination, defined by the percentage of hypocotyl and leaf emergence at 3 days, with short plasma treatment (< 120 s) and all applied doses of electron beam treatment (8–60 kGy). However, even the lowest dose of electron beam treatment at 8 kGy in this study caused root abnormalities in seedlings, suggesting a detrimental effect on the seed tissue. Seeds treated with cold plasma had an eroded seed coat and increased seed wettability compared to electron beam treated seeds. However, these effects cannot explain the increase in the germination capacity of seeds as this was observed for both techniques. Future studies should focus on the investigation of the mechanisms causing accelerated seed germination and root abnormalities by characterizing the molecular and physiological impact of cold plasma and electron beam on seed tissue.
A molecular test based on RT-LAMP for rapid, sensitive and inexpensive colorimetric detection of SARS-CoV-2 in clinical samples
Authors: Amaral Catarina , Antunes Wilson , Moe Elin , Duarte Américo G. , Lima Luís M. P. , Santos Cristiana , Gomes Inês L. , Afonso Gonçalo S. , Vieira Ricardo , Teles Helena Sofia S. , Reis Marisa S. , da Silva Manuel A. Ramalho , Henriques Ana Margarida , Fevereiro Miguel , Ventura M. Rita , Serrano Mónica , Pimentel Catarina 
Published: August 2021

AbstractUntil there is an effective implementation of COVID-19 vaccination program, a robust testing strategy, along with prevention measures, will continue to be the most viable way to control disease spread. Such a strategy should rely on disparate diagnostic tests to prevent a slowdown in testing due to lack of materials and reagents imposed by supply chain problems, which happened at the beginning of the pandemic. In this study, we have established a single-tube test based on RT-LAMP that enables the visual detection of less than 100 viral genome copies of SARS-CoV-2 within 30 min. We benchmarked the assay against the gold standard test for COVID-19 diagnosis, RT-PCR, using 177 nasopharyngeal RNA samples. For viral loads above 100 copies, the RT-LAMP assay had a sensitivity of 100% and a specificity of 96.1%. Additionally, we set up a RNA extraction-free RT-LAMP test capable of detecting SARS-CoV-2 directly from saliva samples, albeit with lower sensitivity. The saliva was self-collected and the collection tube remained closed until inactivation, thereby ensuring the protection of the testing personnel. As expected, RNA extraction from saliva samples increased the sensitivity of the test. To lower the costs associated with RNA extraction, we performed this step using an alternative protocol that uses plasmid DNA extraction columns. We also produced the enzymes needed for the assay and established an in-house-made RT-LAMP test independent of specific distribution channels. Finally, we developed a new colorimetric method that allowed the detection of LAMP products by the visualization of an evident color shift, regardless of the reaction pH.
Effect of L-shaped heat source and magnetic field on heat transfer and irreversibilities in nanofluid-filled oblique complex enclosure
Authors: Zhang Xiao-Hong , Saeed Tareq , Algehyne Ebrahem A. , El-Shorbagy M. A. , El-Refaey Adel M. , Ibrahim Muhammad 
Published: August 2021

AbstractIn this paper, the natural convection heat transfer of water/alumina nanofluid is investigated in a closed square cavity. An oblique magnetic field is applied on the cavity of angle $$\gamma$$
γ
. There is also radiation heat transfer in the cavity. The cavity includes a high-temperature source of L-shape. A low-temperature source as a quadrant of a circle is placed at the corner of the cavity. All other walls are well insulated. The novelty of this work is a low-temperature obstacle embedded in the cavity. Simulations are conducted with a Fortran code based on the control volume method and simple algorithm. Entropy generation rate, Bejan number, and heat transfer are studied by changing different parameters. Results indicate that the highest rates of heat transfer and entropy generation have occurred for the perpendicular magnetic field at high values of the Rayleigh number. At these Rayleigh numbers, the minimum value of the Bejan number is obtained for 15° magnetic field. The magnetic field variation can lead to a change up to 53% in Nusselt number and up to 34% in generated entropy. In a weak magnetic field, the involvement of the radiation heat transfer mechanism leads to an increase in the heat transfer rate so that the Nusselt number can be increased by ten units considering the radiation heat transfer when there is no magnetic field. The maximum heat transfer rate occurs in the horizontal cavity and the minimum value in the cavity of 60° angle. For water, these values are 10.75 and 9.98 for 0 and 60 angles, respectively. Moreover, a weak magnetic field increases the heat transfer rate in the absence of the radiation mechanism, while it is reduced by considering a strong magnetic field.
A simple mathematical model for the evaluation of the long first wave of the COVID-19 pandemic in Brazil
Authors: Tang Yuanji , Serdan Tamires D. A. , Alecrim Amanda L. , Souza Diego R. , Nacano Bruno R. M. , Silva Flaviano L. R. , Silva Eliane B. , Poma Sarah O. , Gennari-Felipe Matheus , Iser-Bem Patrícia N. , Masi Laureane N. , Tang Sherry , Levada-Pires Adriana C. , Hatanaka Elaine , Cury-Boaventura Maria F. , Borges Fernanda T. , Pithon-Curi Tania C. , Curpertino Marli C. , Fiamoncini Jarlei , Leandro Carol Gois , Gorjao Renata , Curi Rui , Hirabara Sandro Massao 
Published: August 2021

AbstractWe propose herein a mathematical model to predict the COVID-19 evolution and evaluate the impact of governmental decisions on this evolution, attempting to explain the long duration of the pandemic in the 26 Brazilian states and their capitals well as in the Federative Unit. The prediction was performed based on the growth rate of new cases in a stable period, and the graphics plotted with the significant governmental decisions to evaluate the impact on the epidemic curve in each Brazilian state and city. Analysis of the predicted new cases was correlated with the total number of hospitalizations and deaths related to COVID-19. Because Brazil is a vast country, with high heterogeneity and complexity of the regional/local characteristics and governmental authorities among Brazilian states and cities, we individually predicted the epidemic curve based on a specific stable period with reduced or minimal interference on the growth rate of new cases. We found good accuracy, mainly in a short period (weeks). The most critical governmental decisions had a significant temporal impact on pandemic curve growth. A good relationship was found between the predicted number of new cases and the total number of inpatients and deaths related to COVID-19. In summary, we demonstrated that interventional and preventive measures directly and significantly impact the COVID-19 pandemic using a simple mathematical model. This model can easily be applied, helping, and directing health and governmental authorities to make further decisions to combat the pandemic.
Evaluation of acetylation and methylation in oral rinse of patients with head and neck cancer history exposed to valproic acid
Authors: Gama Ricardo Ribeiro , Arantes Lidia Maria Rebolho Batista , Sorroche Bruna Pereira , De Marchi Pedro , Melendez Matias Eliseo , Carvalho Raiany Santos , de Lima Marcos Alves , Vettore André Luiz , Carvalho André Lopes 
Published: August 2021

AbstractEvaluate the biological action of valproic acid in the acetylation of histones and in the methylation of tumor suppressor genes via oral rinse in patients with a previous history of head and neck squamous cell carcinoma (HNSCC). Forty-two active or former smokers were included in this randomized, double-blind, placebo-controlled trial. Oral rinse samples were collected prior to treatment with valproic acid or placebo and after 90 days of treatment. The methylation status of five tumor suppressor genes and histone acetylation were evaluated by pyrosequencing and ELISA techniques, respectively. Differences between the 90-day and baseline oral rinse acetylation and methylation results were analyzed by comparing groups. Thirty-four patients were considered for analysis. The mean percentage adherence in the valproic and placebo groups was 93.4 and 93.0, respectively (p = 0.718). There was no statistically significant difference between groups when comparing the medians of the histone acetylation ratio and the methylation ratio for most of the studied genes. A significant reduction in the DCC methylation pattern was observed in the valproic group (p = 0.023). The use of valproic acid was safe and accompanied by good therapeutic adherence. DCC methylation was lower in the valproic acid group than in the placebo group.
shRNA transgenic swine display resistance to infection with the foot-and-mouth disease virus
Authors: Hu Wenping , Zheng Haixue , Li Qiuyan , Wang Yuhang , Liu Xiangtao , Hu Xiaoxiang , Liu Wenjie , Liu Shen , Chen Zhisheng , Feng Wenhai , Cai Xuepeng , Li Ning 
Published: August 2021

AbstractFoot-and-mouth disease virus (FMDV) is one of the most important animal pathogens in the world. FMDV naturally infects swine, cattle, and other cloven-hoofed animals. FMD is not adequately controlled by vaccination. An alternative strategy is to develop swine that are genetically resistant to infection. Here, we generated FMDV-specific shRNA transgenic cells targeting either nonstructural protein 2B or polymerase 3D of FMDV. The shRNA-positive transgenic cells displayed significantly lower viral production than that of the control cells after infection with FMDV (P < 0.05). Twenty-three transgenic cloned swine (TGCS) and nine non-transgenic cloned swine (Non-TGCS) were produced by somatic cell nuclear transfer (SCNT). In the FMDV challenge study, one TGCS was completely protected, no clinical signs, no viremia and no viral RNA in the tissues, no non-structural antibody response, another one TGCS swine recovered after showing clinical signs for two days, whereas all of the normal control swine (NS) and Non-TGCS developed typical clinical signs, viremia and viral RNA was determined in the tissues, the non-structural antibody was determined, and one Non-TGCS swine died. The viral RNA load in the blood and tissues of the TGCS was reduced in both challenge doses. These results indicated that the TGCS displayed resistance to the FMDV infection. Immune cells, including CD3+, CD4+, CD8+, CD21+, and CD172+ cells, and the production of IFN-γ were analyzed, there were no significant differences observed between the TGCS and NS or Non-TGCS, suggesting that the FMDV resistance may be mainly derived from the RNAi-based antiviral pathway. Our work provides a foundation for a breeding approach to preventing infectious disease in swine.
DNA sequence and community structure diversity of multi-year soil fungi in Grape of Xinjiang
Authors: Xue Feng , Liu Tong 
Published: August 2021

AbstractThis study is designed to understand the community structure and diversity of fungi in the rhizosphere soil of grape. As the sample for this study, the rhizosphere soil of Crimson seedless grape with different planting years was collected from Shihezi in Xinjiang to carry out high-throughput sequencing, by which the complete sequence of soil fungi DNA was identified, and accordingly, the richness and diversity index of fungi were determined. The results showed that the dominant phyla of fungi in the grape rhizosphere soil with different planting years were Ascomycota and Basidiomycota, and the dominant classes of fungi were Sordariomycetes and Dothideomycetes. Soil organic matter, total potassium, total nitrogen and available phosphorus were the main soil fertility factors affecting the abundance and diversity of soil fungal communities, among which soil organic matter had the most significant influence. In addition, the fungal diversity and richness were highest in the middle layer (20–35 cm) of the grape rhizosphere soil with 12 planting years and lowest in the lower layer (35–50 cm) of the grape rhizosphere soil with 5 planting years. Linear discriminant analysis suggested that there were more biomarkers in the vineyard rhizosphere soil with 10 planting years, which meant there were more fungal communities with significant difference in the soil, especially in the middle layer (20–35). The results of this study can provide data reference and theoretical basis for improving vineyard soil quality, evaluating soil microecological effects and improving ecological environment of vineyard soil.
Hematopoietic upstream stimulating factor 1 deficiency is associated with increased atherosclerosis susceptibility in LDL receptor knockout mice
Authors: Hoekstra Menno , Ren Baoyan , Laurila Pirkka-Pekka , Hildebrand Reeni B. , Soronen Jarkko , Frodermann Vanessa , Li Zhuang , Boon Mariëtte R. , Geerling Janine J. , Rensen Patrick C. N. , Jauhiainen Matti , Van Eck Miranda 
Published: August 2021

AbstractTotal body upstream stimulatory factor 1 (USF1) deficiency in mice is associated with brown adipose tissue activation and a marked protection against the development of obesity and atherosclerotic lesions. Functional expression of USF1 has also been detected in monocytes and monocyte-derived macrophages. In the current study we therefore tested whether selective hematopoietic USF1 deficiency can also beneficially impact the development of atherosclerosis. For this purpose, LDL receptor knockout mice were transplanted with bone marrow from USF1 knockout mice or their wild-type littermate controls and subsequently fed a Western-type diet for 20 weeks to stimulate atherosclerotic lesion development. Strikingly, absence of USF1 function in bone marrow-derived cells was associated with exacerbated blood leukocyte (+ 100%;P < 0.01) and peritoneal leukocyte (+ 50%;P < 0.05) lipid loading and an increased atherosclerosis susceptibility (+ 31%;P < 0.05). These effects could be attributed to aggravated hyperlipidemia, i.e. higher plasma free cholesterol (+ 33%;P < 0.001) and cholesteryl esters (+ 39%;P < 0.001), and the development of hepatosteatosis. In conclusion, we have shown that hematopoietic USF1 deficiency is associated with an increased atherosclerosis susceptibility in LDL receptor knockout mice. These findings argue against a contribution of macrophage-specific USF1 deficiency to the previously described beneficial effect of total body USF1 deficiency on atherosclerosis susceptibility in mice.