bluuebunny/crossref_metadata_2025_split huggingface dataset:
The impact of the Corona crisis on the environmental behaviors of different socioeconomic groups
Authors: Peleg-Mizrachi Meital , Tal Alon 
Published: May 2021

Abstract
The Corona pandemic led to changes in consumptions patterns, which are both positive and negative. Past research suggests that crises present opportunities for adopting sustainable consumption practices. Alternatively, they tend to increase frugality which can marginalize environmental considerations. This study extends research conducted in 2018 evaluating the environmental impacts of consumption patterns among Israel’s different socioeconomic deciles. The present research returned to the same respondents, during the first lockdown, assessing how consumptions patterns among different socioeconomic deciles, and support for different environmental policy options were influenced by the Corona crisis. The findings show that the poorest deciles increased their environmentally destructive behavior, while wealthier deciles showed modest improvements. All deciles displayed greater frugality in purchasing. Support was greater for policy interventions framed as environmental than for taxes on daily consumer products. The findings confirm the need for environmental programs which make sustainable consumption more readily accessible to poorer socioeconomic groups.
Response of Batang Piaman Variety of Rice Crops to Fertilizers in Suboptimal Rice Field
Authors: Rozen N , Kasim M , Dwipa I 
Published: May 2021

Abstract
Suboptimal land is a problematic land for rice crop growth. This is because the nutrient content in the soil is very low but the content of iron or aluminium elements is so high, that causes toxicity to plants, and then inhibited plant growth. Therefore, it is necessary to add organic fertilizer to the soil, one of which is cow manure that is available at all times and easy to obtain. In addition to cow manure, kieserite is also important to be given into the soil because kieserite contains magnesium nutrients that are badly needed for plant growth. This research purposes to see the best response of rice plants of Batang Piaman variety to fertilizer given to increase growth and yield on suboptimal land. This study used Completely Randomized Design with 3 fertilizer treatments [100% inorganic fertilizer, cow manure 5 tons/ha+150kg/ha kieserite, and 10 tons/ha of cow manure without kieserite]. Each treatment is replicated 5 times. The data is analyzed with a variety of variance and if the results differ noticeably followed by Honestly Significant Difference [HSD] Post Hoc Test in level 5%. Observations had made on some quantitative parameters. From the results of the study obtained that the best response from rice plants of Batang Piaman variety is obtained in the treatment of cow manure 5ton/ha+150kg/ha kieserite that provides high growth of plants 90 cm and the total number tillers of rice as 34 stems and a yield of 6.52 tons/ha on suboptimal rice field.
Fractionation of secondary metabolites from Serratia plymuthica UBCF_13 based on polarity properties
Authors: Hamzah N A , Fatiah R , Jamsari J 
Published: May 2021

Abstract

Serratia plymuthica UBCF_13 bacteria produce compounds capable of suppressing the growth of various pathogenic fungi. There are several types of compounds produced by these bacteria. The compounds produced by S. plymuthica bacteria have different abilities and characteristics. So, to determine the capabilities and characteristics of each compound, a compound separation technique or method is needed. This study was conducted to determine the optimal mobile phase composition for the separation of compounds. Optimization of the mobile phase was carried out by spotting the extracellular extract of S. plymuthica on thin layer chromatography (TLC) using several comparisons of ethyl acetate with hexane and ethyl acetate with methanol. The separation technique in this research will be useful for further study of the antifungal compound Serratia plymuthica UBCF_13, such as the identification and characterization of each of the compounds it produces. In this study, the best mobile phase was not found in the separation of compounds. There are several mobile phases that separate the compounds, for further test the elution gradient method is used.
Endophytic bacterial consortia as biocontrol of purple blotch and plant growth promoters of shallots
Authors: Resti Z , Warnita  , Liswarni Y 
Published: May 2021

Abstract
Endophytic bacteria have been reported to have roles as biocontrol agents and plant growth promoters. The application of endophytic bacteria in the form of mixed culture [consortia] will provide better effectiveness than when applied alone. This study aimed to obtain a consortium of endophytic bacteria that is effective as a biological control for purple blotch [Alternariaporri], promote the growth and yield of shallots. The research was arranged in a completely randomized design [CRD] consisting of 7 treatments with three replications. The consortium used was a mixture of two strains of Bacillus sp., two strains of B. cereus, two strains of Serratia marcescens, Bacillus subtilis, Azotobacter, Azospirillum, and P. fluorescens. The variables observed in this study included disease progression [incubation period, disease incidence, and disease severity], plant growth [number of leaves and plant height], and yield [fresh weight and dry weight of bulbs]. The results showed that the C consortium [Bacillus sp. strain SJI, Bacillus sp. strain HI, Serratia marcescens strain ULG1E4 and Serratia marcescens strain JBIE3] and G consortium [Bacillus sp. strain SJI, Bacillus sp. strain HI, Bacillus subtilis, Pseudomonas fluorescens, Serratia marcescens strain ULG1E4 and Serratiamarcescens strain JBIE3] were the best consortia in suppressing purple blotch disease, as well as in improving the growth and yield of shallots.
Test liquid smoke toxicity for cocoa skin [Theobroma Cacao L.] with the BSLT method at different pyrolysis temperatures
Authors: Budaraga I K , Putra D P 
Published: May 2021

Abstract
Cocoa is one of the leading plantation sector commodities in West Sumatra. Cocoa skin waste is still of little use. One of the uses of cocoa pods can be used as raw material for making liquid smoke. The purpose of this study was to determine the toxicity properties of liquid smoke of cocoa pod skin with the BLST method at different pyrolysis temperatures. Testing the toxicity of liquid smoke of cocoa skin using the Brine Shrimp Lethality Test (BSLT) method. The results LC50 number of cocoa shell liquid smoke at pyrolysis temperature (200°C at 10% water content) was 11,858.58 ppm, pyrolysis temperature (200°C at 15% water content) at 13,094.23 ppm, pyrolysis temperature (200°C at 20 water content %) of 13,373.94 ppm, pyrolysis temperature (200°C at 25% water content) of 15,703.52 ppm. The next a pyrolysis temperature of 300°C 10% water content of 11,604,26 ppm, pyrolysis temperature of 300°C 15% water content of 11,673,05 ppm, pyrolysis temperature of 300°C 20% water content of 13,373,94 ppm, pyrolysis temperature of 25% water content of 13,373.94 ppm. Furthermore, at a pyrolysis temperature of 400°C at a level of 10% at 9,213.73 ppm, a pyrolysis temperature at 400°C at a level of 15% at 13,094237 ppm, a pyrolysis temperature at 400°C at a level of 20% at 13,373.94 ppm, a pyrolysis temperature at a level of 25% at 12,493.63 ppm. All the results of the process of making liquid cocoa skin smoke at different pyrolysis temperatures show the results of liquid smoke with toxic properties classified as non-toxic.
Probiotic research as highest antimicrobial Listeria monocytogenes from virgin coconut oil [VCO] Padang West Sumatra
Authors: Syukur S , Syafrizayanti  , Ismet M , Zulaiha S , Zulkifli  
Published: May 2021

Abstract
Virgin Coconut Oil [VCO] is a natural oil, made from fresh coconut milk fermentation without adding chemicals and other additives. Several Lactic acids Bacteria [LAB], have been isolate and characterization as probiotic. Several 5 different VCO sample products in local Padang Market have been determined. Specifics MRS medium has been used for LAB isolation and morphological characterization. The samples code are A, B, C, D, and E for 5 VCO samples. The highest total colony of LAB is from Samples A, which is about 1377 ×107 CFU/mL. 18 isolates have been identified as Gram-positive bacteria while 8 bacteria’s are coccus and others form basil. All 18 LAB were grown at pH 2, and showing a resistance of about 49 %. Only 50 % of LAB resistant 0,3% oxgall for 4 hours. 3 isolate namely isolate A-1, A-3, and D-1 were showed the highest antimicrobial activities against Listeria monocytogenes with holozone averages of 26 mm. The hydrophobicity against xylene was also determined. The potential A-3 colonies are the highest percentages grown about 19% and resistant to several antibiotics such as Ampicillin, Chloramphenicol, Erythromycin, and Tetracycline. Molecular identification for potential A-3 using 16S RNA, showing phylogenetics species as Lactobacillus fermentum strain KF7.
Sensory analysis and physicochemical characteristics of carica [Vasconcellea pubescens] fruit leather with seaweed [Eucheuma cottonii] addition
Authors: Sukotjo S , Nurani D , Juliansyah M A 
Published: May 2021

Abstract
Carica [Vasconcellea pubescens] is a type of papaya fruit mostly cultivated in the Dieng area, Central Java, Indonesia. Carica is smaller than regular papaya, has an orange to yellow color, the flesh is tough, has a lot of sap and the taste is a bit sour. One of the products that can be made from Carica is fruit leather. Fruit leather is the processed fruit puree in the form of a thin sheet with a thickness of 2-3 mm, resembles a skin, has a plastic texture, can be rolled, and has a distinctive fruit flavor. The seaweed [Eucheuma cottonii] which mostly contains carrageenan, is added in the process to give the elastic texture to the fruit leather. The purposes of this study are to compare the effect of seaweed concentration on Carica fruit preferred by panelists and to analyze the physicochemical characteristics of the fruit leather. The process of making Carica fruit leather is done by preparing Carica puree, adding sugar, adding seaweed, heating, molding, and drying. The statistical analysis shows no effect of seaweed concentration on the panelists’ preference test. The product has a sweet and slightly sour taste, yellowish to light brown color with the aroma of Carica. The physical analysis shows that the fruit leather has a thickness of 1.8-2.0 mm, a chewy texture, and a yield of 22.46-27.21%. Chemical analysis shows that the water content is 22.18-25.31%, the vitamin C content is 0.94-1.69 mg/100g, and dietary fiber is 5.19-10.31%.
Determination of growth and antioxidant activity assay of in vitro gamma-irradiated Tacca leontopetaloides shoots
Authors: Martin A F , Hapsari B W , Ermayanti T M 
Published: May 2021

Abstract

Tacca leontopetaloides (L.) is a tuberous plant with high starch content similar to those found in potatoes. However, despite its potential as a functional crop, the plant is still underutilized due to limited cultivation by farmers. Besides, a class of specific metabolites found in this plant has the potential as an anticancer drug. In this study, we sought genetic improvement of T. leontopetaloides using Gamma-ray irradiation. The aim of the research was to observe growth, antioxidant activities, and phytochemical properties after Gamma-ray irradiation treatment at 20 Gy dose. We grew the irradiated culture on MS medium supplemented with 0.5 mg/L BAP or 0.5 mg/L Kinetin. We recorded the growth every week for eight weeks. After eight weeks of culture, the total fresh biomass, antioxidant activities, and phytochemical properties were calculated. The results showed that a modest growth reduction was found on irradiated plant culture compared with the control culture. Antioxidant IC50 on irradiated culture was lower than the control, which was indicating higher antioxidant activities on irradiated culture. However, no change of phytochemical properties was observed on both irradiated and control culture measured qualitatively. This study reveals a new candidate of T. leontopetaloides mutant induced by gamma-ray irradiation with higher antioxidant activities than its wild type.
Development of Rice Varieties Adaptive to Nontidal Swampland using MABC: Growth Characteristics of Parent Plant and F1 Result
Authors: Suwignyo R A , Irmawati I , Hose F , Aulia S L 
Published: May 2021

Abstract
Lowland swamps have enormous potential and have been proven to provide significant contributions as a buffer in the national food security system. Rice farmers in non-tidal swamplands of South Sumatra plant rice at the end of the rainy season, because they wait for the water to recede, and therefore rice plants are very vulnerable to submerged stress in the vegetative phase and drought stress in the generative phase. This study aims to obtain rice varieties that are adaptive to these conditions and superior to lowland swamp agro-ecosystems. The research was conducted at the Plant Physiology Laboratory and Green House of Department of Agronomy, Faculty of Agriculture, Sriwijaya University. In this study, reciprocal crosses were carried out between Inpago 5 and Inpara 8 rice varieties. Inpago 5 variety was used because it has tolerant characters to drought stress in the generative phase and also has high production in farmers’ nontidal swampland. Inpara 8 has a Sub1 gene so that resistant to submerged stress in the vegetative phase. The results showed that both varieties had different characters in their growth and yield. Both varieties were crossed reciprocally and the success of crossing was more than 50%. F1 plants will be backcrossed with their female parents to get BC1F1 plants, and then selected using the MABC method [foreground selection, phenotypic selection, and background selection]. From the results of the MABC selection process, there will be BC1F1 plants with the genome proportion close to the female parent and confirmed to have the Sub1 gene.
Digital mapping and soil carbon stock distribution on various landuse of tropical peatland in Pesisir Selatan, West Sumatra
Authors: Naspendra Z , Aprisal A , Hijri N , Harianti M , Junaidi  
Published: May 2021

AbstractIndonesia has the largest tropical peatlands in the world covered an area of 14.91 million ha. Peatlands play an important role in global carbon sequestration. This study aimed to: a] map the peatland in Pesisir Selatan, Sumatra Barat calculate the soil carbon stock in the peatlands on various land use and peat thickness and c] identify the relationship of soil characteristics to the soil carbon. We investigated thirty soil samples in Pesisir Selatan. The land-use types on peatland in Pesisir Selatan consisted of forest [GH], shrub [GS], oil palm plantations [GPs], annual cropland[GLp], and bareland [GLp]. The results showed that the total area of peatlands in Pesisir Selatan is 78,998.74 ha, while the total amount of soil carbon stocks is 244 million tonnes, and the sequence follows GPs > GS > GH > GT > GLp. The average value of soil carbon stock is 3,090.89 per ha, the sequence follows GH > GS > GT > GPs > GLp. Hence, the average amount of soil carbon stock based on depth is 8,529 tons for peat depth > 600cm, 4,082 tons for peat depth 300-600 cm, and 525 tons for peat depth 0-300 cm. Differences in average values of soil carbon stock per ha are highly influenced by the differences in peat thickness. The dynamics of total carbon show a higher its content in the subsurface layer rather than in the surface layer. The soil carbon is linearly correlated with water content and it is inversely proportional to bulk density.
Potential of biochar bamboo and sub-bituminous coal as amendment of acid mineral soils for improving the growth of arabica coffee [Coffea arabica l.] seedlings
Authors: Prasetyo T B , Naspendra Z , Maulana A , Solfianti M , Krisna S D , Herviyanti  
Published: May 2021

Abstract
Improvement the characteristics of acid mineral soil [Ultisols] is needed to support plant productivity. However, the utilization of biochar combined with sub-bituminous in improving ultisol is still limited. Therefore, this research was conducted by using treatments: 1] Control 0% Sub-bituminous [SBC] + 0% Bamboo Biochar [B]; 2] 100% SBC [20 ton.ha−1]; 3] 75% SBC + 25% B; 4] 50% SBC + 50% B; 5] 25% SBC + 75% B; and 6] 100% B [20 ton.ha-1] This study was designed using Completely Randomized Design [CRD] with 3 replications. The results showed that the application of combined biochar and sub-bituminous had a significant effect in improving ultisol’s fertility characteristics. There are two treatments that provide the most significant results for improving soil properties, namely 100% sub-bituminous treatment with a significant effect in increasing pH [1.3 pH units], Organic carbon [3.34%], Total N [0.12%], CEC [10.87 cmol/kg and Ca2+ [1.2%]. However, 50% sub-bituminous + 50% bamboo biochar treatment have a significant effect in increasing Available P [1.1 ppm], CEC [9.04 cmol/kg K+ [0.51 cmol/kg], Ca2+ [1.37 cmol/kg], and Mg2+ [1.27 cmol/kg]. In addition, application of 50% sub-bituminous + 50% bamboo biochar gave the best result for the plant height, branches and leaves of the coffee plants.
Performance of Some Soil Physical Properties of Arabica Coffee Plantation in Solok Regency
Authors: Adrinal  , Gusmini  , Darfis I. , Putri Elsa L. 
Published: May 2021

Abstract
Arabica coffee also known as mountain coffee is one of the plantation crops that has great potential to be developed on agribusiness scale in Solok Regency due to its suitability on agro-climate region for growth and also may provide great expectation of financial benefit for farmers as well as for economical aspect for the region. A study of soil physical properties under different age of arabica coffee was conducted from April to November 2020 in Lembah Gumanti, Pantai Cermin and Danau Kembar subdistricts, Solok Regency, West Sumatra. The aim of this study was to analyse some of soil organic matter, particle size distribution (soil texture), bulk density, total porosity, and aggregate stability index on different ages from five locations of arabica coffee plantation. Results showed that the soil physical properties of the arabica coffee of 1, 4, 8, and 13 years old, and secondary forest has been changed. The only the soil permeability of the 20-40 cm depth showed significantly different. These may be due to the difference in organic matter application and management in different age of arabica coffee plantation.
Phytoremediation Agents of Rice Biochar and Cage Fertilizer in Ex-Gold Mining and The Sunflower Growth
Authors: Gusmini G , Adrinal A , Putri E L , Panji R. , Husna F E 
Published: May 2021

Abstract
Degraded former gold mining land so that soil fertility is very minimal. Ameliorant mining with an open system causes increased oxidation of sulfurous minerals which reduces nutrient levels and accumulation of heavy metals on the soil surface. This research aims to improve the physical properties of the former gold mining area. This study used a completely randomized design method of 6 treatments with 4 replications. The treatment dose used was 40 tons/ha. The results of this study are that the application of rice husk biochar with manure can provide the best nutrients and growth for sunflower plants.
Erosion hazard index [EHI] on different land use in sub-watershed Kaos, Jambi
Authors: Ramadhan S , Hermansah  , Rusman B , Yasin S 
Published: May 2021

Abstract
Soil degradation that occurs in watershed Batanghari is generally caused by accelerated erosion due to land conversion. Sub-watershed Kaos is one of the sub-watershed from watershed Batanghari that has forest conversion to oil palm plantations is 7,846 Ha from the period 1995-2018. This research aims to assess the Erosion Hazard Index [EHI] on forest land use, land clearing, and oil palm plantation in Sub-Watershed Kaos-Jambi. The data analysis method used the survey method and erosion prediction using the Universal Soil Loss Equation [USLE] model. The EHI was counted by the amount of soil eroded [ton/ha/year] divided by soil erosion that can be tolerated [ton/ha/year]. The result of this research showed that the forest’s erosion value is 9.80 ton/ha/year, land clearing is 249.17 ton/ha/year and oil palm plantation is 89.12-122.94 ton/ha/year. The soil erosion can be tolerated on the forest is 36.54 ton/ha/year, land clearing is 57.71 ton/ha/year and oil palm plantation is 54.42-57.49 ton/ha/year. Furthermore, the lowest value of EHI is 0.27 [low grade] and the highest value of EHI is 4.32 [high grade], whereas the EHI value on oil palm plantation is between 1.64 to 2.14 [medium grade]. The EHI in Sub-Watershed Kaos is in low, medium, and high categories. The medium grade and high grade of The EHI will cause soil degradation, such as decreasing soil quality and also loss of organic matter and soil nutrients because of erosion and runoff. Therefore, it must apply soil conservation and agrotechnology application in land clearing and oil palm plantation.
In-silico construction of potential cis-acting elements from PD_CbNPR1 with T7 minimal promoter
Authors: Oktavioni M , Tjong D H , Jamsari J 
Published: May 2021

Abstract
A hybrid promoter is a synthetic promoter engineering technique that has a function as a tool for cis-acting elements validation. The hybrid promoter is assembled by combining the minimal promoter [MP] with fragments containing cis-acting elements. PD_CbNPR1 from Capsicum annuum cv. Berangkai has several potential cis-acting elements for the regulation of plant resistance gene expression but has not been validated in the in-vitro assay. The potential elements are the W-Box motif which has three variations, then the WLE1 and RAV1AAT which have one variation motif. Sequences containing the cis-acting elements of PD_CbNPR1 were synthesized as fragment g-block. The MP used was the T7 Promoter in the pET28a[+] plasmid. The g-block fragment will be fused in the upstream region of the T7 Promoter. Before constructing the hybrid promoter in-vitro, it is necessary to construct the T7 promoter with cis-acting elements in-silico. The software used in this research was the plasmid editor ApE vers.2.0.36. The in-silico construct of the T7 promoter with cis-acting elements aimed to obtain plasmid construction designs that will be assembled in-vitro. The constructs obtained in this study consisted of six variations, in the form of a circular plasmid contains the potential cis-acting element. These constructs are called constructs A [WBox1-WBox2-WBox3-WLE1-RAV1AAT + pET28a[+]], B [WBox1 + pET28a[+]], C [WBox2 + pET28a[+]], D [WBox3 + pET28a[+]], E [WLE1 + pET28a[+]], and F [RAV1AAT + pET28a[+]]. The six constructs will be used in further study to regulate gene expression, as a tool to validate the cis-acting elements’ role in-vitro.
Cloning Reference Gene rpoB in Serratia plymuthica UBCF_13
Authors: Trivano R , Fatiah R , Jamsari J 
Published: May 2021

Abstract
The rpoB gene is one of the housekeeping genes in the Serratia plymuthica UBCF_13. This gene is usually used as a reference gene in qPCR analysis to quantify the expression level of the target gene. In the absolute analysis, the reference gene qPCR was cloned to create a standard curve for calculating the copy number of the target gene expression level. The method used in this study was to clone a partial rpoB gene through the ligation to pGEM®-T Easy plasmid cloning vector and transform it to E. coli Dh5a to proliferation recombinant plasmid. The results of this study indicated the reference rpoB gene was successfully cloned using pGEM®-T Easy with the size of insert gene 118 bp length.