bluuebunny/crossref_metadata_2025_split huggingface dataset:
Providing domperidone throughout lactation enhances sow lactation performance
Authors: Farmer Chantal , Palin Marie-France 
Published: June 2021

Abstract
The goal of this project was to determine the effects of domperidone given throughout lactation on hormonal and metabolic status, lactational performance, and gene expression in mammary epithelial cells of sows. Second parity sows were divided in two treatment groups: 1) daily intramuscular injections with canola oil (Control, CTL, n = 24), or 2) daily intramuscular injections with 0.5 mg/kg body weight (BW) of domperidone (DOMP, n = 23). Injections were given at 08h05 starting the day after farrowing until weaning. Over the first 4 d of treatment, DOMP sows also received 0.5 mg/kg BW of domperidone per os twice daily, whereas CTL sows were fed the vehicle. Litter size was standardized to 11 ± 1 within 24 h of birth and piglets were weighed at birth, 24 h postpartum, and on days 7, 22 (weaning on day 23), 35, and 56. Sow feed intake was recorded daily. Representative milk samples were obtained aseptically on day 21 of lactation from 15 sows per treatment for compositional analyses and milk fat globules were used to measure mRNA abundances of various genes. Jugular blood samples were obtained from all sows on days 2, 8, 16, and 23 of lactation to measure concentrations of prolactin, insulin-like growth factor-1 (IGF-1), leptin, adiponectin, insulin, glucose, urea, and free fatty acids (FFA). Concentrations of prolactin (P &lt; 0.001) and FFA (P &lt; 0.01) were increased in DOMP compared with CTL sows, whereas concentrations of insulin were decreased (P &lt; 0.05). Urea concentrations were increased by treatment (P &lt; 0.05) on days 16 and 23 of lactation, and those of IGF-1 were increased (P &lt; 0.01) on day 16. Piglets from DOMP sows were heavier than those from CTL sows on day 22 (P &lt; 0.01). Milk composition was unaffected by treatment. The mRNA abundance in milk fat globules for casein beta and whey acidic protein were lower (P ≤ 0.05) in DOMP than CTL sows. The long form of the prolactin receptor and the signal transducer and activator of transcription 5A mRNA abundances tended to be lower (P &lt; 0.10) in DOMP than CTL sows. In conclusion, hyperprolactinemia induced by domperidone during lactation affected the endocrine and metabolite status of sows and stimulated growth of their suckling piglets.
Cold-priming causes dampening of oxylipin biosynthesis and signalling during the early cold- and light-triggering response ofArabidopsis thaliana
Authors: Bittner Andras , Hause Bettina , Baier Margarete 
Published: June 2021

AbstractCold-priming uncouples cold and light regulation of otherwise tightly co-regulated genes. In this study, we focused on the early regulatory processes in Arabidopsis within the first 2 h in cold and in high light after a 5-d lag-phase at 20 °C and 24 h cold-priming at 4 °C. Priming quickly modified gene expression in a trigger-specific manner. In the early stress-response phase during cold and high-light triggering, it reduced the regulatory amplitudes of many up- and down-regulated genes. A third of the priming-regulated genes were jasmonate-sensitive, including the full set of genes required for oxylipin biosynthesis. Analysis of wild-type and mutant plants based on qPCR demonstrated that biosynthesis of the jasmonic acid (JA) precursor 12-oxo phytenoic acid (OPDA) relative to the availability of JA dampened the response of the genes for oxylipin biosynthesis. In oxylipin biosynthetic mutants, cold-priming more strongly affected genes involved in the biosynthesis of OPDA than in its conversion to JA. In addition, priming-dependent dampening of the triggering response was more linked to OPDA than to regulation of the JA concentration. Spray application of OPDA prior to triggering counteracted the priming effect. Regulation of the oxylipin hub was controlled by modulation of the oxylipin-sensitivity of the genes for OPDA biosynthesis, but it was insensitive to priming-induced accumulation of thylakoid ascorbate peroxidase, thus identifying a parallel-acting cold-priming pathway.
Chameleon brokers: A translocal take on migration industries in the Thai-Swedish wild berry business
Authors: van Eerbeek Peter , Hedberg Charlotta 
Published: June 2021

AbstractMigrant brokers constitute a substantial node in the industries that underpin contemporary global migration processes, including seasonal labour migrants in agri-food businesses. This article adds a translocal perspective to the role of migrant brokers, while emphasising the multi-sited embeddedness of brokers in sending and receiving countries, and their role in sustaining transnational migration flows. The example of the Swedish wild berry industry shows how two groups of translocal brokers operate in multi-sited space, first, Thai women brokers residing in rural Sweden, and second, local brokers, residing in rural Thailand. This article emphasises how translocal brokers are giving migration industries access to multi-sited embeddedness, both at the site of recruitment in Thai villages and at the site of work in Sweden. The translocal embeddedness is noticed in how moral economies and trust are at play in recruitment processes, and how moral economies are then transferred across space to the site of work. Also, it accentuates how translocal brokers are main subjects, in how their biographical histories are creating translocal relations across space. Lastly, we show how spatial divisions of labour are creating social hierarchies among workers, where the brokers themselves incorporate shifting, ‘chameleon’ roles in multi-sited space. The analysis brings the moral complexity of brokers to the surface, while showing how the social relations of their ‘moral economies’ are commodified within profit-seeking migration industries.
Gas-phase metallicity gradients of TNG50 star-forming galaxies
Authors: Hemler Z S , Torrey Paul , Qi Jia , Hernquist Lars , Vogelsberger Mark , Ma Xiangcheng , Kewley Lisa J , Nelson Dylan , Pillepich Annalisa , Pakmor Rüdiger , Marinacci Federico 
Published: June 2021

ABSTRACT
We present the radial gas-phase, mass-weighted metallicity profiles and gradients of the TNG50 star-forming galaxy population measured at redshifts z = 0–3. We investigate the redshift evolution of gradients and examine relations between gradient (negative) steepness and galaxy properties. We find that TNG50 gradients are predominantly negative at all redshifts, although we observe significant diversity among these negative gradients. We determine that the gradients of all galaxies grow more negative with redshift at a roughly constant rate of approximately $-0.02\ \mathrm{dex\, kpc^{-1}}/\Delta z$. This rate does not vary significantly with galaxy mass. We observe a weak negative correlation between gradient (negative) steepness and galaxy stellar mass at z &lt; 2. However, when we normalize gradients by a characteristic radius defined by the galactic star formation distribution, we find that these normalized gradients do not vary significantly with either stellar mass or redshift. We place our results in the context of previous simulations and show that TNG50 high-redshift gradients are more negative than those of models featuring burstier feedback, which may further highlight high-redshift gradients as important discriminators of galaxy formation models. We also find that z = 0 and z = 0.5 TNG50 gradients are consistent with the gradients observed in galaxies at these redshifts, although the preference for flat gradients observed in redshift z ≳ 1 galaxies is not present in TNG50. If future JWST (James Webb Space Telescope) and ELT (Extremely Large Telescope) observations validate these flat gradients, it may indicate a need for simulation models to implement more powerful radial gas mixing within the ISM (interstellar medium), possibly via turbulence and/or stronger winds.
Molecular Clocks and Archeogenomics of a Late Period Egyptian Date Palm Leaf Reveal Introgression from Wild Relatives and Add Timestamps on the Domestication
Authors: Pérez-Escobar Oscar A , Bellot Sidonie , Przelomska Natalia A S , Flowers Jonathan M , Nesbitt Mark , Ryan Philippa , Gutaker Rafal M , Gros-Balthazard Muriel , Wells Tom , Kuhnhäuser Benedikt G , Schley Rowan , Bogarín Diego , Dodsworth Steven , Diaz Rudy , Lehmann Manuela , Petoe Peter , Eiserhardt Wolf L , Preick Michaela , Hofreiter Michael , Hajdas Irka , Purugganan Michael , Antonelli Alexandre , Gravendeel Barbara , Leitch Ilia J , Jimenez Maria Fernanda Torres , Papadopulos Alexander S T , Chomicki Guillaume , Renner Susanne S , Baker William J 
Published: June 2021

Abstract
The date palm, Phoenix dactylifera, has been a cornerstone of Middle Eastern and North African agriculture for millennia. It was first domesticated in the Persian Gulf, and its evolution appears to have been influenced by gene flow from two wild relatives, P. theophrasti, currently restricted to Crete and Turkey, and P. sylvestris, widespread from Bangladesh to the West Himalayas. Genomes of ancient date palm seeds show that gene flow from P. theophrasti to P. dactylifera may have occurred by ∼2,200 years ago, but traces of P. sylvestris could not be detected. We here integrate archeogenomics of a ∼2,100-year-old P. dactylifera leaf from Saqqara (Egypt), molecular-clock dating, and coalescence approaches with population genomic tests, to probe the hybridization between the date palm and its two closest relatives and provide minimum and maximum timestamps for its reticulated evolution. The Saqqara date palm shares a close genetic affinity with North African date palm populations, and we find clear genomic admixture from both P. theophrasti, and P. sylvestris, indicating that both had contributed to the date palm genome by 2,100 years ago. Molecular-clocks placed the divergence of P. theophrasti from P. dactylifera/P. sylvestris and that of P. dactylifera from P. sylvestris in the Upper Miocene, but strongly supported, conflicting topologies point to older gene flow between P. theophrasti and P. dactylifera, and P. sylvestris and P. dactylifera. Our work highlights the ancient hybrid origin of the date palms, and prompts the investigation of the functional significance of genetic material introgressed from both close relatives, which in turn could prove useful for modern date palm breeding.
A Crowdsourced Consensus on Supratotal Resection Versus Gross Total Resection for Anatomically Distinct Primary Glioblastoma
Authors: Khalafallah Adham M , Rakovec Maureen , Bettegowda Chetan , Jackson Christopher M , Gallia Gary L , Weingart Jon D , Lim Michael , Esquenazi Yoshua , Zacharia Brad E , Goldschmidt Ezequiel , Ziu Mateo , Ivan Michael E , Venteicher Andrew S , Nduom Edjah K , Mamelak Adam N , Chu Ray M , Yu John S , Sheehan Jason P , Nahed Brian V , Carter Bob S , Berger Mitchel S , Sawaya Raymond , Mukherjee Debraj 
Published: June 2021

Abstract

BACKGROUND
Gross total resection (GTR) of contrast-enhancing tumor is associated with increased survival in primary glioblastoma. Recently, there has been increasing interest in performing supratotal resections (SpTRs) for glioblastoma.

OBJECTIVE
To address the published results, which have varied in part due to lack of consensus on the definition and appropriate use of SpTR.

METHODS
A crowdsourcing approach was used to survey 21 neurosurgical oncologists representing 14 health systems nationwide. Participants were presented with 11 definitions of SpTR and asked to rate the appropriateness of each definition. Participants reviewed T1-weighed postcontrast and fluid-attenuated inversion-recovery magnetic resonance imaging for 22 anatomically distinct glioblastomas. Participants were asked to assess the tumor location's eloquence, the perceived equipoise of enrolling patients in a randomized trial comparing gross total to SpTR, and their personal treatment plans.

RESULTS
Most neurosurgeons surveyed (n = 18, 85.7%) agree that GTR plus resection of some noncontrast enhancement is an appropriate definition for SpTR. Overall, moderate inter-rater agreement existed regarding eloquence, equipoise, and personal treatment plans. The 4 neurosurgeons who had performed >10 SpTRs for glioblastomas in the past year were more likely to recommend it as their treatment plan (P < .005). Cases were divided into 3 anatomically distinct groups based upon perceived eloquence. Anterior temporal and right frontal glioblastomas were considered the best randomization candidates.

CONCLUSION
We established a consensus definition for SpTR of glioblastoma and identified anatomically distinct locations deemed most amenable to SpTR. These results may be used to plan prospective trials investigating the potential clinical utility of SpTR for glioblastoma.
Surgery for Degenerative Cervical Myelopathy: A Nationwide Registry-Based Observational Study With Patient-Reported Outcomes
Authors: Gulati Sasha , Vangen-Lønne Vetle , Nygaard Øystein P , Gulati Agnete M , Hammer Tommy A , Johansen Tonje O , Peul Wilco C , Salvesen Øyvind O , Solberg Tore K 
Published: June 2021

Abstract

BACKGROUND
Indications and optimal timing for surgical treatment of degenerative cervical myelopathy (DCM) remain unclear, and data from daily clinical practice are warranted.

OBJECTIVE
To investigate clinical outcomes following decompressive surgery for DCM.

METHODS
Data were obtained from the Norwegian Registry for Spine Surgery. The primary outcome was change in the neck disability index (NDI) 1 yr after surgery. Secondary endpoints were the European myelopathy score (EMS), quality of life (EuroQoL 5D [EQ-5D]), numeric rating scales (NRS) for headache, neck pain, and arm pain, complications, and perceived benefit of surgery assessed by the Global Perceived Effect (GPE) scale.

RESULTS
We included 905 patients operated between January 2012 and June 2018. There were significant improvements in all patient-reported outcome measures (PROMs) including NDI (mean −10.0, 95% CI −11.5 to −8.4, P < .001), EMS (mean 1.0, 95% CI 0.8-1.1, P < .001), EQ-5D index score (mean 0.16, 95% CI 0.13-0.19, P < .001), EQ-5D visual analogue scale (mean 13.8, 95% CI 11.7-15.9, P < .001), headache NRS (mean −1.1, 95% CI −1.4 to −0.8, P < .001), neck pain NRS (mean −1.8, 95% CI −2.0 to −1.5, P < .001), and arm pain NRS (mean −1.7, 95% CI −1.9 to −1.4, P < .001). According to GPE scale assessments, 229/513 patients (44.6%) experienced “complete recovery” or felt “much better” at 1 yr. There were significant improvements in all PROMs for both mild and moderate-to-severe DCM. A total of 251 patients (27.7%) experienced adverse effects within 3 mo.

CONCLUSION
Surgery for DCM is associated with significant and clinically meaningful improvement across a wide range of PROMs.
Care Facilitation Advances Movement Along the Hepatitis C Care Continuum for Persons With Human Immunodeficiency Virus, Hepatitis C, and Substance Use: A Randomized Clinical Trial (CTN-0064)
Authors: Metsch Lisa R , Feaster Daniel J , Gooden Lauren K , Masson Carmen , Perlman David C , Jain Mamta K , Matheson Tim , Nelson C Mindy , Jacobs Petra , Tross Susan , Haynes Louise , Lucas Gregory M , Colasanti Jonathan A , Rodriguez Allan , Drainoni Mari-Lynn , Osorio Georgina , Nijhawan Ank E , Jacobson Jeffrey M , Sullivan Meg , Metzger David , Vergara-Rodriguez Pamela , Lubelchek Ronald , Duan Rui , Batycki Jacob N , Matthews Abigail G , Munoz Felipe , Jelstrom Eve , Mandler Raul , del Rio Carlos 
Published: June 2021

Abstract

Background
Direct-acting antivirals can cure hepatitis C virus (HCV). Persons with HCV/HIV and living with substance use are disadvantaged in benefiting from advances in HCV treatment.

Methods
In this randomized controlled trial, participants with HCV/HIV were randomized between February 2016 and January 2017 to either care facilitation or control. Twelve-month follow-up assessments were completed in January 2018.
Care facilitation group participants received motivation and strengths-based case management addressing retrieval of HCV viral load results, engagement in HCV/HIV care, and medication adherence. Control group participants received referral to HCV evaluation and an offer of assistance in making care appointments. Primary outcome was number of steps achieved along a series of 8 clinical steps (eg, receiving HCV results, initiating treatment, sustained virologic response [SVR]) of the HCV/HIV care continuum over 12 months postrandomization.

Results
Three hundred eighty-one individuals were screened and 113 randomized. Median age was 51 years; 58.4% of participants were male and 72.6% were Black/African American. Median HIV-1 viral load was 27 209 copies/mL, with 69% having a detectable viral load. Mean number of steps completed was statistically significantly higher in the intervention group vs controls (2.44 vs 1.68 steps; χ 2 [1] = 7.36, P = .0067). Men in the intervention group completed a statistically significantly higher number of steps than controls. Eleven participants achieved SVR with no difference by treatment group.

Conclusions
The care facilitation intervention increased progress along the HCV/HIV care continuum, as observed for men and not women. Study findings also highlight continued challenges to achieve individual-patient SVR and population-level HCV elimination.

Clinical Trials Registration
NCT02641158.
Absence of Antibody Reponses and Severe COVID-19 in Patients on Hemodialysis Following mRNA Vaccination
Authors: Michael Miriam B , Mahgoub Siham M , Khan Reiad , Mellman Thomas A , Mere Constance C , Mehari Alem , Naab Tammey J , Nwagowugwu Uzoamake , Ihaegwara Susan , Maxwell Celia J 
Published: June 2021

Abstract
Inpatient dialysis patients cannot isolate, resulting in a higher rate of coronavirus disease 2019 (COVID-19) infections, with increased severity and higher mortality rate [1]. We present 2 African American dialysis patients who developed severe COVID-19 infections after vaccination. Both patients had not mounted antibody response to the COVID-19 vaccine or to hepatitis B vaccination.
Development of an In Vitro Chloroplast Splicing System: Sequences Required for Correct pre-mRNA Splicing
Authors: Inaba-Hasegawa Keiko , Ohmura Ayumi , Nomura Masayo , Sugiura Masahiro 
Published: June 2021

Abstract
Chloroplast genomes in land plants include approximately 20 intron-containing genes. Most of the introns are similar to the group II introns found in fungi, algae and some bacteria, but no self-splicing has been reported. To analyze splicing reactions in chloroplasts, we developed a tobacco (Nicotiana tabacum) chloroplast-based in vitro system. We optimized the splicing reaction using atpF precursor messenger RNA (pre-mRNA). Our system requires a high ATP concentration, whereas ATP is not necessary for self-splicing group II introns. Self-splicing group II introns possess two exon-binding sites (EBS1 and 2) complementary to two intron-binding sites (IBS1 and 2) in the 3′ end of 5′ exons, which are involved in 5′ splice-site selection. Using our in vitro system and atpF pre-mRNA, we analyzed short sequences corresponding to the above EBSs and IBSs. Mutation analyses revealed that EBS1–IBS1 pairing is essential, while EBS2–IBS2 pairing is important but not crucial for splicing. The first 3′ exon nucleotide determines the 3′ splice sites of self-splicing introns. However, mutations to this nucleotide in atpF pre-mRNA did not affect splicing. This result suggests that the mechanism underlying chloroplast pre-mRNA splicing differs partly from that mediating the self-splicing of group II introns.
Domain Extension and Ideal Elements in Mathematics
Authors: Bellomo Anna 
Published: June 2021

AbstractDomain extension in mathematics occurs whenever a given mathematical domain is augmented so as to include new elements. Manders argues that the advantages of important cases of domain extension are captured by the model-theoretic notions of existential closure and model completion. In the specific case of domain extension via ideal elements, I argue, Manders’s proposed explanation does not suffice. I then develop and formalize a different approach to domain extension based on Dedekind’s Habilitationsrede, to which Manders’s account is compared. I conclude with an examination of three possible stances towards extensions via ideal elements.
Reduced cognitive ability in people with rheumatoid arthritis compared with age-matched healthy controls
Authors: Gwinnutt James M , Toyoda Task , Jeffs Stephen , Flanagan Emma , Chipping Jacqueline R , Dainty Jack R , Mioshi Eneida , Hornberger Michael , MacGregor Alex 
Published: June 2021

AbstractObjectiveThe aim was to compare the cognitive ability of people with RA with healthy controls (HCs).MethodsPeople with RA were recruited from the Norfolk Arthritis Register (NOAR), a population-based cohort study of people with inflammatory arthritis. Data on aged-matched HCs (people with no cognitive impairment) came from the comparison arm of The Dementia Research and Care Clinic Study (TRACC). People with RA and HCs performed a range of cognitive ability tasks to assess attention, memory, verbal fluency, language, visuospatial skills, emotional recognition, executive function and theory of mind. A score of &lt;88 on the Addenbrooke’s Cognitive Examination III was considered cognitive impairment. Scores were compared using linear regression adjusting for age, sex, smoking status, education, BMI, anxiety and depression.ResultsThirty-eight people with RA [mean (S.D.) age: 69.1 (8.0) years; 25 (65.8%) women] were matched with 28 HCs [mean (S.D.) age: 68.2 (6.4) years; 15 (53.6%) women]. Twenty-three (60.5%) people with RA were considered to have mild cognitive impairment [mean (S.D.) Addenbrooke’s Cognitive Examination III: RA = 85.2 (7.4), HC = 96.0 (2.5)]. People with RA had impairments in memory, verbal fluency, visuospatial functioning, executive function and emotional recognition in faces compared with HCs, after adjustment for confounders.ConclusionPeople with RA had cognitive impairments in a range of domains. People with RA might benefit from cognitive impairment screening to allow for early administration of appropriate interventions.
Neural substrates of shared visual experiences: a hyperscanning fMRI study
Authors: Yoshioka Ayumi , Tanabe Hiroki C , Sumiya Motofumi , Nakagawa Eri , Okazaki Shuntaro , Koike Takahiko , Sadato Norihiro 
Published: June 2021

Abstract
Sharing experience is a fundamental human social cognition. Since visual experience is a mental state directed toward the world, we hypothesized that sharing visual experience is mediated by joint attention (JA) for sharing directedness and mentalizing for mental state inferences. We conducted a hyperscanning functional magnetic resonance imaging with 44 healthy adult volunteers to test this hypothesis. We employed spoken-language-cued spatial and feature-based JA tasks. The initiator attracts the partner’s attention by a verbal command to a spatial location or an object feature to which the responder directs their attention. Pair-specific inter-individual neural synchronization of task-specific activities was found in the right anterior insular cortex (AIC)–inferior frontal gyrus (IFG) complex, the core node of JA and salience network, and the right posterior superior temporal sulcus, which represents the shared categories of the target. The right AIC-IFG also showed inter-individual synchronization of the residual time-series data, along with the right temporoparietal junction and dorsomedial prefrontal cortex—the core components for mentalization and the default mode network (DMN). This background synchronization represents sharing the belief of sharing the situation. Thus, shared visual experiences are represented by coherent coordination between the DMN and salience network linked through the right AIC-IFG.