bluuebunny/crossref_metadata_2025_split huggingface dataset:
Construction of stable mouse artificial chromosome from native mouse chromosome 10 for generation of transchromosomic mice
Authors: Abe Satoshi , Honma Kazuhisa , Okada Akane , Kazuki Kanako , Tanaka Hiroshi , Endo Takeshi , Morimoto Kayoko , Moriwaki Takashi , Hamamichi Shusei , Nakayama Yuji , Suzuki Teruhiko , Takehara Shoko , Oshimura Mitsuo , Kazuki Yasuhiro 
Published: October 2021

AbstractMammalian artificial chromosomes derived from native chromosomes have been applied to biomedical research and development by generating cell sources and transchromosomic (Tc) animals. Human artificial chromosome (HAC) is a precedent chromosomal vector which achieved generation of valuable humanized animal models for fully human antibody production and human pharmacokinetics. While humanized Tc animals created by HAC vector have attained significant contributions, there was a potential issue to be addressed regarding stability in mouse tissues, especially highly proliferating hematopoietic cells. Mouse artificial chromosome (MAC) vectors derived from native mouse chromosome 11 demonstrated improved stability, and they were utilized for humanized Tc mouse production as a standard vector. In mouse, however, stability of MAC vector derived from native mouse chromosome other than mouse chromosome 11 remains to be evaluated. To clarify the potential of mouse centromeres in the additional chromosomes, we constructed a new MAC vector from native mouse chromosome 10 to evaluate the stability in Tc mice. The new MAC vector was transmitted through germline and stably maintained in the mouse tissues without any apparent abnormalities. Through this study, the potential of additional mouse centromere was demonstrated for Tc mouse production, and new MAC is expected to be used for various applications.
Nanoscale analysis of superconducting Fe(Se,Te) epitaxial thin films and relationship with pinning properties
Authors: Scuderi Mario , Pallecchi Ilaria , Leo Antonio , Nigro Angela , Grimaldi Gaia , Ferdeghini Carlo , Spinella Corrado , Guidolin Marco , Trotta Antonio , Braccini Valeria 
Published: October 2021

AbstractThe process of developing superconducting materials for large scale applications is mainly oriented to optimize flux pinning and the current carrying capability. A powerful approach to investigate pinning properties is to combine high resolution imaging with transport measurements as a function of the magnetic field orientation, supported by a pinning modelling. We carry out Transmission Electron Microscopy, Electron Energy Loss Spectroscopy and critical current measurements in fields up to 16 T varying the angle between the field and c-axis of Fe(Se,Te) epitaxial thin films deposited on CaF2 substrates. We find evidence of nanoscale domains with different Te:Se stoichiometry and/or rotated and tilted axes, as well as of lattice distortions and two-dimensional defects at the grain boundaries. These elongated domains are tens of nm in size along the in-plane axes. We establish a correlation between these observed microstructural features and the pinning properties, specifically strongly enhanced pinning for the magnetic field oriented in-plane and pinning emerging at higher fields for out-of-plane direction. These features can be accounted for within a model where pinning centers are local variations of the critical temperature and local variations of the mean free path, respectively. The identification of all these growth induced defects acting as effective pinning centers may provide useful information for the optimization of Fe(Se,Te) coated conductors.
Food insecurity arises the likelihood of hospitalization in patients with COVID-19
Authors: Ariya Mohammad , Karimi Jalal , Abolghasemi Somayeh , Hematdar Zeinab , Naghizadeh Mohammad Mehdi , Moradi Maryam , Barati-Boldaji Reza 
Published: October 2021

AbstractThe World Health Organization (WHO) has declared the Corona pandemic as a public health emergency. This pandemic affects the main pillars of food security. This study aimed to investigate the relationship between food insecurity and the probability of hospitalization and the length of the recovery period after getting COVID-19. The cross-sectional study was performed through the census on COVID-19 patients diagnosed in Fasa, Iran. Informed consent, demographic, and food security questionnaire were completed over the phone. Then, all patients were followed up until recovery. Data were analyzed using SPSS26 and Chi-square test, t-test, and logistic regression (P < 0.05). In this study, 219 COVID-19 patients [100 (54.7%) male and 119 (54.3%) female] with a mean age of 40.05 ± 15.54 years old were examined. Possibility of hospitalization and the length of the recovery period of more than one month was significantly longer in the food-insecure group (P = 0.001) and (P = 0.37), respectively, but the mean length of hospital stay in the two groups was not significantly different (P = 0.76). After adjusting for all confounding variables, people with food insecurity were 3.9 times more likely to be hospitalized than those with food security. Overall, we observed that food-insecure people were significantly more likely to be hospitalized than the secure group.
Risk assessment and source apportionment of trace elements in multiple compartments in the lower reach of the Jinsha River, China
Authors: He Wenyan , Li Fei , Yu Jiang , Chen Min , Deng Yun , Li Jia , Tang Xiliang , Chen Zhuoyu , Yan Zhongluan 
Published: October 2021

AbstractStudies on trace element (TE) pollution in abiotic matrices have typically focused on water, sediment, and soil, either separately or in pairs. The importance of multi-media connectivity has been ignored. This study analyzed the concentrations of 6 TEs in three connected environmental compartments of a 28-km section of the lower reach of the Jinsha River. The ecological risk posed by TEs was higher in soil than in sediment. The contribution of exposure pathways to human health risk were ranked as ingestion > dermal contact > inhalation. An improved regional environmental risk index (RERI) method was then developed to evaluate the comprehensive risk on both ecology and human health caused by TEs. The average RERI value was generally higher in the wet season (0.42) than in the dry (0.41) and dry-to-wet transition seasons (0.08) because of the combined effects of the high TE concentrations in riparian soil and the long exposure time. Source apportionment indicated that industrial activities, weathering of parent rock, and agricultural activities were possible sources of TEs in this region. The methods and results of this study could inform local environmental management and provide references for similar cases wherein multiple compartments of river systems should be considered.
Effectiveness of empirical anti-pseudomonal antibiotics for recurrent COPD exacerbation: a multicenter retrospective cohort study
Authors: Shiroshita Akihiro , Miyakoshi Chisato , Tsutsumi Shunta , Shiba Hiroshi , Shirakawa Chigusa , Sato Kenya , Matsushita Shinya , Kimura Yuya , Tomii Keisuke , Ohgiya Masahiro , Kataoka Yuki 
Published: October 2021

AbstractAlthough frequent chronic obstructive pulmonary disease (COPD) exacerbation has been associated with the isolation of Pseudomonas aeruginosa (PA) in sputum cultures, it remains unknown whether the empirical use of anti-pseudomonal antibiotics can improve outcomes in patients with frequent COPD exacerbations. This multicenter retrospective cohort study evaluated whether the empirical use of anti-pseudomonal antibiotics improves the length of the hospital stay in patients with recurrent COPD exacerbation (≥ 2 admissions from April 1, 2008 to July 31, 2020). For statistical analysis, a log-linked Gamma model was used. Parameters were estimated using a generalized estimating equation model with an exchangeable correlation structure accounting for repeated observations from a single patient. Covariates included age, body mass index, home oxygen therapy use, respiratory rate, heart rate, oxygen use on admission, mental status, systemic steroid use, activities of daily living, and the number of recurrences. Hospital-specific effects were specified as fixed effects. In total, 344 patients and 965 observations of recurrent COPD exacerbations were selected. Anti-pseudomonal antibiotics were used in 173 patients (18%). The estimated change in the length of the hospital stay between anti-pseudomonal and non-anti-pseudomonal antibiotics groups was 0.039 days [95% confidence interval; − 0.083, 0.162]. Anti-pseudomonal antibiotics could not shorten the length of the hospital stay.
Development of peptides targeting receptor binding site of the envelope glycoprotein to contain the West Nile virus infection
Authors: Mertinková Patrícia , Mochnáčová Evelína , Bhide Katarína , Kulkarni Amod , Tkáčová Zuzana , Hruškovicová Jana , Bhide Mangesh 
Published: October 2021

AbstractWest Nile virus (WNV), re-emerging neurotropic flavivirus, can cross the blood–brain barrier (BBB) and cause fatal encephalitis and meningitis. Infection of the human brain microvascular endothelial cells (hBMECs), building blocks of the BBB, represents the pivotal step in neuroinvasion. Domain III (DIII) of the envelope (E) glycoprotein is a key receptor-binding domain, thus, it is an attractive target for anti-flavivirus strategies. Here, two combinatorial phage display peptide libraries, Ph.D.-C7C and Ph.D.-12, were panned against receptor-binding site (RBS) on DIII to isolate peptides that could block DIII. From series of pannings, nine peptides (seven 7-mer cyclic and two 12-mer linear) were selected and overexpressed in E. coli SHuffle T5. Presence of disulfide bond in 7-mer peptides was confirmed with thiol-reactive maleimide labeling. Except for linear peptide 19 (HYSWSWIAYSPG), all peptides proved to be DIII binders. Among all peptides, 4 cyclic peptides (CTKTDVHFC, CIHSSTRAC, CTYENHRTC, and CLAQSHPLC) showed significant blocking of the interaction between DIII and hBMECs, and ability to neutralize infection in cultured cells. None of these peptides showed toxic or hemolytic activity. Peptides identified in this study may serve as potential candidates for the development of novel antiviral therapeutics against WNV.
State selective classical electron capture cross sections in Be4+  + H(1s) collisions with mimicking quantum effect
Authors: Ziaeian Iman , Tőkési Károly 
Published: October 2021

AbstractWe present state-selective electron capture cross sections in collision between Be4+ and ground state hydrogen atom. The n- and nl-selective electron capture cross sections are calculated by a three-body classical trajectory Monte Carlo method (CTMC) and by a classical simulation schema mimicking quantum features of the collision system. The quantum behavior is taken into account with the correction term in the Hamiltonian as was proposed by Kirschbaum and Wilets (Phys Rev A 21:834, 1980). Calculations are carried out in the projectile energy range of 1–1000 keV/amu. We found that our model for Be4+ + H(1s) system remarkably improves the obtained state-selective electron capture cross sections, especially at lower projectile energies. Our results are very close and are in good agreement with the previously obtained quantum–mechanical results. Moreover, our model with simplicity can time efficiently carry out simulations where maybe the quantum mechanical ones become complicated, therefore, our model should be an alternative way to calculate accurate cross sections and maybe can replace the quantum–mechanical methods.
Impact of one-rod levonorgestrel implant on the blood chemistry profile
Authors: Gunardi Eka R. , Surya Raymond , Syafitri Inayah , Pasidri Yogi 
Published: October 2021

AbstractThe aim of this study was to investigate the effect of a one-rod levonorgestrel implant on the blood chemistry profile, including random blood glucose (RBG), haemoglobin (Hb), alanine transferase (ALT), aspartate transferase (AST), and the lipid profile, including total cholesterol, high-density lipoprotein (HDL), low-density lipoprotein (LDL), and triglycerides. This prospective cohort study was conducted at Raden Saleh Clinic, Jakarta, from 2010 to 2012. The implants were inserted subdermally in 30 patients. The subjects were evaluated every 6 month up to 2 years. Bivariate analysis using t-test or Wilcoxon signed rank test was performed for all variables. p < 0.05 was considered a significant value. The Hb, RBG, AST, and lipid profile levels were significantly different before and 6 months after one-rod implant insertion (p < 0.05). However, for 24 months, all of the parameters were still within normal limits and did not differ clinically. One-rod levonorgestrel implant insertion has a minimal effect on all blood chemistry profiles.
XT-type DNA hydrogels loaded with VEGF and NGF promote peripheral nerve regeneration via a biphasic release profile
Authors: Liu Songyang , Liu Yijun , Zhou Liping , Li Ci , Zhang Meng , Zhang Fengshi , Ding Zhentao , Wen Yongqiang , Zhang Peixun 
Published: October 2021

Preparation of XT-type DNA hydrogel loaded with VEGF and NGF which could achieve their biphasic release.The repair effect and mechanism of the XT-type DNA hydrogels on a 10 mm peripheral nerve defect were investigated in vitro and in vivo.
Electro-oxidative C–H amination of heteroarenes with aniline derivatives via radical–radical cross coupling
Authors: Peng Xichao , Zhao Junhao , Ma Guojian , Wu Yan , Hu Shiyu , Ruan Zhixiong , Feng Pengju 
Published: October 2021

A protocol for the C–H amination of heteroarenes via an electro-oxidative radical cross coupling process could access variety of hetero-arene derivatives and tolerate a wide range of functional groups, and is amenable to the gram scale synthesis.
CNN‐combined graph residual network with multilevel feature fusion for hyperspectral image classification
Authors: Guo Wenhui , Xu Guixun , Liu Weifeng , Liu Baodi , Wang Yanjiang 
Published: October 2021

AbstractThe application of graph convolutional networks (GCN) in hyperspectral image (HSI) classification has become a promising method, thanks to its flexible convolution operation in any irregular image region. For the classification of HSI, GCN can extract more superpixel‐level features with a topological structure, in comparison to the traditional convolutional neural networks (CNNs) using fixed square kernels distilling pixel‐level features. To fully leverage the different levels of features, this study proposes a novel deep network referred to as a CNN‐combined graph residual network (GRN), which integrates the multilevel graph residual module and spectral‐spatial features continuous learning module. During the extraction of topology information using the former module, HSI pixels are divided into superpixels and served as input nodes of the module to reduce the computational complexity and obtain the multilevel spatial relevance between adjacent superpixels. Besides, for the latter module, the spectral‐spatial features are learnt continuously, which could obtain the finer pixel‐level features. Finally, the captured spectral‐spatial features of different levels are concatenated. This strategy could not only adequately utilize the correlation and difference of adjacent spatial but also obtain the finer and more valuable spectral‐spatial information, which makes a significant boost in the HSI classification. Additionally, the experiment results demonstrate the superiority and availability of the GRN on three benchmark datasets of HSI, compared with the state‐of‐the‐art methods for the classification of HSI.
The Fornax Cluster VLT Spectroscopic Survey
Authors: Napolitano N. R. , Gatto M. , Spiniello C. , Cantiello M. , Hilker M. , Arnaboldi M. , Tortora C. , Chaturvedi A. , D’Abrusco R. , Li R. , Paolillo M. , Peletier R. , Saifollahi T. , Spavone M. , Venhola A. , Capaccioli M. , Longo G. 
Published: October 2021

Context. Substructures in stellar haloes are a strong prediction of galaxy formation models in ΛCDM. Cold streams such as those from small satellite galaxies are extremely difficult to detect and kinematically characterize. The COld STream finder Algorithm (COSTA) is a novel algorithm able to find streams in the phase space of planetary nebulae (PNe) and globular cluster (GC) populations. COSTA isolates groups of (N) particles with small velocity dispersion (between 10 km s−1 and ∼120 km s−1) using an iterative (n) sigma-clipping over a defined number of (k) neighbor particles.
Aims. We applied COSTA to a catalog of PNe and GCs from the Fornax Cluster VLT Spectroscopic Survey (FVSS) within ∼200 kpc from the cluster core in order to detect cold substructures and characterize their kinematics (mean velocity and velocity dispersion).
Methods. We selected more than 2000 PNe and GCs from the FVSS catalogs and adopted a series of optimized setups of the COSTA parameters based on Montecarlo simulations of the PN and GC populations to search for realistic stream candidates. We find 13 cold substructures with velocity dispersion ranging from ∼20 to ∼100 km s−1, which are likely associated either to large galaxies or to ultra-compact dwarf (UCD) galaxies in the Fornax core.
Results. The luminosities of these streams show a clear correlation with internal velocity dispersion, and their surface brightness correlates with their size and distance from the cluster center, which is compatible with the dissipative processes producing them. However, we cannot exclude that some of these substructures formed by violent relaxation of massive satellites that finally merged into the central galaxy. Among these substructures we have: (1) a stream connecting NGC 1387 to the central galaxy, NGC 1399, previously reported in the literature; (2) a new giant stream produced by the interaction of NGC 1382 with NGC 1380 and (possibly) NGC 1381; (3) a series of streams kinematically connected to nearby UCDs; and (4) clumps of tracers with no clear kinematical association to close cluster members.
Conclusions. We show evidence for a variety of cold substructures predicted in simulations. Most of the streams are kinematically connected to UCDs, supporting the scenario that they can be remnants of disrupted dwarf systems. However, we also show the presence of long coherent substructures connecting cluster members and isolated clumps of tracers possibly left behind by their parent systems before these merged into the central galaxy. Unfortunately, the estimated low-surface brightness of these streams does not allow us to find their signatures in the current imaging data and deeper observations are needed to confirm them.
Folklore, Animal Self-Medication, and Phytotherapy–Something Old, Something New, Something Borrowed, Some Things True
Authors: Huffman Michael A. 
Published: October 2021

AbstractThe use of medicines was long considered by Western schools of thought to be a a domain unique to humans; however, folklore/Traditional Ecological Knowledge (TEK) from around the world suggests that animals have also long provided inspiration for the discovery of some medicinal plants used to treat humans and their livestock. Searching for medicinal knowledge from animals depends on the recognition of their ability to select and effectively use medicinal plants to prevent or actively ameliorate disease and other homeostatic imbalances. The interdisciplinary field of animal self-medication is providing scientific evidence for this ability in species across the animal kingdom and lends support to animal-origin medicinal plant folklore and recent ethnomedicinal information. Here, 14 case studies of purported animal-inspired plant medicines used by cultures around the world are presented together with ethnomedicinal and pharmacological evidence. Based on this evidence, the
diversity and potential mode of self-medicative behaviors are considered. Over 20 animal species, including llama, sloth and jaguar in South America, reindeer and yak in Eurasia, langur and macaque in Asia, and chimpanzee, wild boar, porcupine and elephant in Africa, are linked to these case studies, representing a variety of potential preventative or therapeutic self-medicative behaviors. These examples provide an important perspective on what is likely to have been a much wider practice in the development of human traditional medicine. A role for animal self-medication research in the rejuvenation of old therapies and possible new discoveries of phytotherapies for human and livestock health is encouraged.
Gibt es eine „Kältekrankheit“ in der europäischen Naturheilkunde?
Authors: Stange Rainer 
Published: October 2021

SummaryDer westlichen Welt entstammende Traditionelle Medizinen benutzen oft das Konzept der Konstitution. Sie kennen hier auch Anteile, die sich mit Wärme und Kälte beschäftigen. Später wurden diese Aspekte unter dem speziellen Begriff Thermokonstitution in allgemeinen Konstitutionslehren berücksichtigt. In der Regel signalisiert eine gesteigerte Kälteempfindlichkeit dabei eine eher schwache Konstitution, die den Träger sowohl für akute Infektionskrankheiten insbesondere der oberen Atemwege, aber auch für chronische Erkrankungen bis hin zu Krebs anfälliger werden lässt. Für die Praxis der Hydro-/Thermotherapie stellt sich die Frage nach der aktuellen Belastbarkeit. Wissenschaftlich sind alle diese wichtigen Fragen leider völlig unzureichend bearbeitet.
Menopause is more than Hot Flashes: What is Missing in Homeopathic Research? A Narrative Review
Authors: Macías-Cortés Emma 
Published: October 2021

AbstractBackground Menopausal complaints are frequently treated with homeopathy in daily practice worldwide. Recently, vasomotor symptoms have been understood to have implications as predictors of other important and long-term outcomes, causing increased risk of mortality and/or disability.Methods A comprehensive search of the literature was conducted to investigate whether homeopathic treatments for menopausal women with vasomotor symptoms have a positive effect on other important health outcomes associated with menopause, such as cardiovascular disease, neurocognitive impairment, metabolic and mood disorders, or osteoporosis.Results Though observational studies have shown encouraging results in reducing the severity and frequency of hot flashes in women treated with homeopathy, few randomized controlled trials have shown positive results. In most of the studies using homeopathy, the primary outcome is reduction in the frequency and severity of hot flashes, and other menopausal complaints are assessed secondarily as a part of the symptoms evaluated in the menopausal scales. Quality of life improves with homeopathic treatments for hot flashes, but there is scarce evidence of the effect of homeopathy on other health outcomes associated with menopause. Limited evidence exists in the case of menopausal women treated with individualized homeopathy for depression and metabolic disorders.Conclusion A more comprehensive approach for treating menopause in routine homeopathic practice constitutes a valuable opportunity to increase knowledge and high-quality research in this field. Future homeopathic research for menopause should be focused on well-designed, double-blind, placebo-controlled, randomized trials as well as on pragmatic trials to show whether homeopathic treatments for vasomotor symptoms can also improve outcomes that are well-known to increase the risk of mortality and/or disability.