bluuebunny/crossref_metadata_2025_split huggingface dataset:
Natural Variation of Dt2 Promoter Controls Plant Height and Node Number in Semi-Determinant Soybeans
Authors: Kou Kun , Su Tong , Wang Yanping , Yang Hui , Du Hao , He Milan , Li Tai , Ma Lixin , Liao Chunmei , Yang Cen , Shi Wenqian , Chen Linnan , Li Yongli , Yang Bize , Kong Lingping , Li Shichen , Wang Lingshuang , Zhao Xiaohui , Lu Sijia , Liu Baohui , Kong Fanjiang , Fang Chao 
Published: March 2021

Abstract
Soybean [Glycine max (L.) Merrill] is one of the most important legume crops and its plant height (PH) is one significant quantitative trait closely related to node number (NN) and internode length (IL) on the main stem, which affect yield together. In this study, we used a recombinant inbred line (RIL) derived from a cross between two semi-determinate stem soybean varieties (Dt1Dt1Dt2Dt2), JKK378 and HXW. A consistent QTL named qPH18 simultaneously controlling PH, NN, and IL was identified, in which this region harbors the semi-determinant gene, Dt2. Sequencing of the promoter of Dt2 from JKK378, identified three polymorphisms relative to HXW, including two SNPs and a 18bp Indel. The expression level of Dt2 in qPH18JKK378 group was lower than that in qPH18HXW group, meanwhile, as a downstream gene, the expression of Dt1 from two groups showed a contrary tendency. Transient transfection assay confirmed that the promoter activity of Dt2 from JKK378 is lower compared to HXW. We speculate that the polymorphisms in the two dominant Dt2 promoter caused difference in expression level of Dt2 and its downstream gene Dt1, hence, affect PH, NN, IL, and grain weight per plant without changing stem growth habit. Compared to PH18HXW allele, qPH18JKK378 allele suppresses the expression of Dt2 gene which releases the expression of Dt1, thus promotes plant node number and enhanced grain yield. These results will be helpful for revealing the mechanism underlying the node number and plant height, the soybean materials and molecular marker will facilitate molecule breeding.
Screening and Characterization of Lactic Acid Bacteria with Broad-Spectrum Antimicrobial Activity from Tibetan Qula, a Yak Milk Cheese
Authors: Zhang Bei , Chen Jun , Wu Guofang , Wang Lei , Qin Guangyong , Pang Huili , Zhang Miao , Liu Yuan , Tan Zhongfang 
Published: March 2021

Abstract
BackgroundLactic acid bacteria with natural, effective antibacterial activity, safe and reliable characteristic, gradually become one of the key technologies in food fermentation applications, food preservation and other fields. In this study, 112 presumptive lactic acid bacteria isolated from Tibetan Qula, a fermented yak cheese popular in the Tibetan plateau, were screened for potential probiotic microorganism with antimicrobial activity.Results12 lactic acid bacteria were found to have antibacterial activity, and strain QZ50 in particular showed broad-spectrum inhibition against pathogenic bacteria, which retained its antibacterial activity after sequential removal of acids and hydrogen peroxide, indicating the production of a broad-spectrum bacteriocin that could inhibite Micrococcus luteus ATCC 28001, Staphylococcus aureus ATCC 26003, Bacillus subtilis ATCC 63501, Escherichia coli ATCC 30105, Pseudomonas aeruginosa ATCC 10104, and Salmonella enterica ATCC 50094. Strain QZ50 was identified as Lactobacillus plantarum based on physicochemical characteristics and 16S rDNA sequencing. And the optimum production conditions were evaluated to obtain the highest yield of plantaricin QZ50. The optimum medium, temperature, initial pH, and inoculum amount for plantaricin QZ50 production were Man, Rogosa, and Sharpe (MRS), 30°C, 6.5, and 3%, respectively. In addition, different C source, N source and stimulating factors in medium show significant effects on plantaricin QZ50 production (P ＜ 0.05). The optimum C and N source were respectively glucose and yeast extract, and 2% Tween 80 contributed highest production of plantaricin QZ50. Plantaricin QZ50 exhibited strong heat stability and remained activity at pH 2.0–8.0. In addition, plantaricin QZ50 was inactivated by pepsin, proteinase K, trypsin, papain, and chymotrypsin.ConclusionsSome strains of Lactobacillus isolated from the Qula in the Tibetan plateau have good antibacterial activity which could be considered as potential probiotic. The strain of Lactobacillus plantarum QZ50, with a broad-spectrum, stable, safe, and natural antibiotic, has potential applications as a food biopreservative.
The Application of Full-Size Three-Dimensional Individual Printed Model Combined with Three-Dimensional Digital Demonstration Can Facilitate Patient’s Preoperative Comprehension to Robotic-Assisted Laparoscopic Partial Nephrectomy
Authors: Yuan Xiaobin , Liu Xiaolei , Jing Qiang , Liu Fan , Zhang Xuhui 
Published: March 2021

Abstract
Background: In this study, it was aimed to evaluate the feasibility and effectiveness of full-size three-dimensional individual printed model (3D-IPM) based on computerized tomography (CT) reconstruction combined with 3D individual digital models (3D-IDMs) for improving the patient’s and their families’ comprehension levels of robotic-assisted laparoscopic partial nephrectomy (RALPN) preoperatively. Methods: Between January 2020 and January 2021, 37 patients underwent RALPN in our institution. 3D individual digital models (3D-IDMs) were reconstructed based on the data of computerized tomography (CT) scanning and full-size 3D-IPMs were fabricated correspondingly. For each patient and his/her closest accompanying immediate family member (CAIFM) (spouse or son/daughter), two semi-structured conversations were held by using CT films (1st conversation) and 3D-IPM combined with 3D-IDM demonstration (2nd one) respectively. The preoperative levels of comprehension were evaluated quantitatively by using a self-made preoperative comprehending score (PCS) in the patients and CAIFMs. Results: All the fabrications of full-size 3D-IPMs and all the operations were technically successful. The total PCS elevated significantly by presenting 3D-IPM combined with 3D-IDM demonstration compared with CT films (42.5 vs 35.5 in patients, P<0.001; 42.9 vs 35.8 in CAIFMs, P<0.001). Sub-PCSs in the evaluating aspects of renal anatomy, mass characteristics, the upcoming RALPN procedure, potential complication risks and prognosis also showed a uniformed climbing pattern with the assistance of 3D-IPM+3D-IDM. Conclusion: The application of 3D-IPM presentation combined with 3D-IDM demonstration can improve the preoperative comprehension of patient and CAIFM to RALPN with more intuitionistic and verisimilar presentation， and can be used in RALPN patient education for increasing patients’ and their families’ cognitive empowerment.
Effectiveness of faith-based interventions on the rate of discharged against medical advice in the tertiary newborn units in Nigeria: A protocol for an open label randomized control trial, the FBI-DAMA Study
Authors: Alao Michael Abel , Ibrahim Olayinka Rasheed , Ogunbosi Oluwatosin Babatunde , Nna Emmanuel Okechukwu , Asinobi Adanze Onyenonachi , Olapegba Peter Olamakinde 
Published: March 2021

Abstract
Background: Discharged against medical advice (DAMA) is a risk factor that often leads to adverse outcomes and hospital re-admissions in neonatal units. Few studies have shown that spiritual/faith-based institutions (FBI) tend to have lower incidence of DAMA compared with public hospitals. Perhaps the holistic approach to patient care that addresses the spiritual needs, the soul and the body component of a being in this setting may account for the observed lower incidence of DAMA. Limited randomized control trials (RCT) exist on faith-based interventions with regards to DAMA in published literature. This study seeks to compare the effectiveness of FBI, social support, religiosity and types of FBI on neonatal DAMA against standard of care in tertiary hospitals in Nigeria. Methods: This RCT will be carried out in 2 public tertiary teaching hospitals in 2 of 6 geopolitical zones in Nigeria. The socio-demographic and clinical details of all patients admitted to the neonatal wards during the study period will be documented. Study participants will be selected through a multistage sampling technique. Subjects will be randomized and allocated to treatment and control arms having established baseline measure of social support and religiosity. Ethical approval was obtained from the State Research Ethics Review Committee. Written informed consent will be obtained from the parents/caregivers prior to patient enrolment. The study will be conducted in line with Declaration of Hesinki 2013. Appropriate statistical tools will be used for data collection and analysis.Discussion:The outcome of this analysis will give insight into the effectiveness of FBI on DAMA. It will also predict the effect of the mediators of parents/caregivers’ religiosity, spirituality, forms of FBI, parents/caregivers’ religious sect and social support on rate of DAMA on neonatal admission in tertiary hospitals in Nigeria. This may help inform Public Health Institutions and Governments’ decisions on the determinants of neonatal DAMA and how to mitigate such outcomes. It is hoped that the evidence from this study may guide policy formulation and guidelines to enhance hospital retention of sick neonates until they are fit for discharge. Trial registration: This study was registered in the Pan Africa Clinical Trial Registry (PACTR202102670906630).
Surgical Ciliated Cyst Secondary to Orthognathic Surgery: Report of three cases and review of the literature
Authors: Díez-Montiel Alberto , Armentia  , Antunez-Conde Raul , Navarro-Cuéllar  , Tousidonis  , Salmeron Jose Ignacio , Antón-Badiola  , Soares Jo Castro , Bonsfills  
Published: March 2021

Abstract
Background: Surgical ciliated cysts (SCC) of the maxilla appear as a delayed complication after surgery in the maxillary sinus, midface osteotomies, traumatic tooth extraction, and maxillary fractures. They are defined as a lytic lesion with entrapment of the pseudostratified columnar epithelium.Report of three cases: We present three patients in which after orthognathic surgery a slowly enlarging asymptomatic swelling mass was developed. On CT, all the cases presented lytic expansive lesions in the alveolar ridge. In the three cases, lesions were completely excised, and upon histological examination, ciliated pseudostratified columnar epithelium with goblet cells was found so they were diagnosed as SCC. An exhaustive review of the medical literature was conducted. The PubMed database was searched for PubMed Central (PMC). 44 references were found, reporting 1555 cases: 11 series and 33 case reports, being the largest series from the Asian countries. The mean age was 46.5 years, and more frequently described in male patients. The most frequent form of presentation was as unilateral unilocular cysts in the posterior maxilla. They were described most frequently after a Caldwell-Luc approach. The average latency time was of 19.9 years.Conclusion: SCC is a rare complication after orthognathic surgery, but large series have been published, so maybe it could be interesting to include these cysts in the next Edition of the World Health Organization Classification of the Head and Neck Tumors: Odontogenic and Maxillofacial Bone Tumors, making clear the difference between SCC and mucoceles to avoid future confusions.
Focusing on the Treatment of COVID-19: A Comprehensive Analysis of 226 Trials Registered on Clinicaltrials.gov and ChiCTR
Authors: Guo Jincai , Xie Hui , Wu Hao 
Published: March 2021

Abstract
Background: The purpose of this study is to analyze the registered clinical trials of COVID-19, and to provide a reference for the clinical treatment of COVID-19. Methods: Chinese ClinicalTrial Registry (ChiCTR) and Clinicaltrials.gov databases were searched for clinical trials of COVID-19, which were registered from inception to February 29, 2020, to screen out the clinical trials on the treatment of COVID-19, and the research units and regions, sample size, study types, study stages, and intervention measures were analyzed. Results: There were 226 clinical trials on COVID-19 in the 2 databases, and all of them were registered by research units in China. The top five registered areas were Hubei, Beijing, Shanghai, Guangdong, and Zhejiang. The study type was as follows: interventional study (207, 91.6%) and observational study (18, 8.0%). Clinical trial staging was as follows: exploratory studies/preliminary trials (91, 40.3%), phase I trials (4, 1.8%), phase II trials (12, 5.3%), phase III trials (12, 5.3%), phase IV trials (47, 20.8%), phase I/II trials (2, 0.9%), phase II/III trials (5, 2.2%), and other trials (57, 25.2%). Intervention measures were as follows: there were 143 (63.3%) trials of western medicine treatment, 50 (22.1%) trials of Chinese medicine treatment, and 21 (9.3%) trials of integrated Chinese medicine treatment and western medicine treatment. Conclusion: Researchers have registered a large number of clinical trials in a short time. The number of existing patients of COVID-19 is not enough to support hundreds of clinical trials. There is a lack of multicenter, randomized, double-blind, placebo-controlled trials.
Ferromagnesian jeffbenite synthesized at 15 GPa and 1200 °C
Authors: Smyth Joseph R. , Wang Fei , Alp E. Ercan , Bell Aaron S. , Posner Esther S. , Jacobsen Steven D. 
Published: March 2021

Abstract
Single crystals of Al-free, ferromagnesian jeffbenite up to 200 µm in size have been synthesized at 15 GPa and 1200 °C in a 1200 tonne multi-anvil press from a starting composition in the forsteritefayalite-magnetite-water system. This phase has the approximate formula Mg2.62Fe0.872+Fe1.633+Si2.88O12 and is observed to coexist with a Ca-free clinopyroxene plus what appears to be quenched melt. The crystal structure has been refined from single-crystal X-ray diffraction data and is similar to that determined for natural Al-bearing jeffbenite, Mg3Al2Si3O12, reported from inclusions in superdeep diamonds. The structure is a tetragonal orthosilicate in space group I42d with a = 6.6449(4) Å, c = 18.4823(14) Å, and is structurally more closely related to zircon than to garnet. The T2 site is larger than T1, shares an edge with the M2 octahedron, and incorporates significant Fe3+. Because of the tetrahedral incorporation of trivalent cations, jeffbenite appears to be compositionally distinct from garnet. Previous speculations that the phase may only occur as a retrograde decompression product from bridgmanite are not supported by its direct synthesis under transition zone conditions. The phase has a calculated density of 3.93 g/cm3, which is indistinguishable from a garnet of comparable composition, and is a possible component in the mantle transition zone under oxidizing conditions or with Al-rich compositions.
Temperature analysis of a novel MAIB reactor during the treatment of wastewater from recycled paper mill
Authors: Zwain Haider M. , Naje Ahmed Samir , Vakili Mohammadtaghi , Dahlan Irvan 
Published: March 2021

Abstract
Anaerobic digestion (AD) is an essential technology for wastewater management, resource recovery and biogas production, and it is considered as an efficient and reliable treatment method for many wastewaters. Operating parameters have been shown to directly affect the stability and treatment performance of AD, especially temperature. For 180 days, the AD of recycled paper mill wastewater (RPMW) was carried out in a modified anaerobic inclining-baffled (MAIB) reactor under various temperature conditions, i.e. 29 °C (low mesophilic), 37 °C (mesophilic) and 55 °C (thermophilic). It was found that total COD removal of 94, 96 and 76%, and methane yields of 0.125, 0.196 and 0.256 L CH4/g CODremoved were attained at temperatures of 29, 37 and 55 °C, respectively. Throughout the three transition periods, the pH level in the MAIB reactor fluctuated slightly within the range of 5.8–6.5 without affecting the system stability. The results concluded that thermophilic condition strongly influenced the MAIB reactor performance, leading to lower COD removal, higher methane yield and gradually recovered pH level.
Detection of water quality failure events at treatment works using a hybrid two-stage method with CUSUM and random forest algorithms
Authors: Riss Gerald , Romano Michele , Memon Fayyaz Ali , Kapelan Zoran 
Published: March 2021

Abstract
Near-real-time event detection is crucial for water utilities to be able to detect failure events in their water treatment works (WTW) quickly and efficiently. This paper presents a new method for an automated, near-real-time recognition of failure events at WTWs by the application of combined statistical process control and machine-learning techniques. The resulting novel hybrid CUSUM event recognition system (HC-ERS) uses two distinct detection methodologies: one for fault detection at the level of individual water quality signals and the second for the recognition of faulty processes at the WTW level. HC-ERS was tested and validated on historical failure events at a real-life UK WTW. The new methodology proved to be effective in the detection of failure events, achieving a high true-detection rate of 82% combined with a low false-alarm rate (average 0.3 false alarms per week), reaching a peak F1 score of 84% as a measure of accuracy. The new method also demonstrated higher accuracy compared with the CANARY detection methodology. When applied to real-world data, the HC-ERS method showed the capability to detect faulty processes at WTW automatically and reliably, and hence potential for practical application in the water industry.
Consumptive water footprints, water use efficiencies and productivities of rice under alternate wetting and drying for Kharagpur, West Bengal, India
Authors: Biswas A. , Mailapalli D. R. , Raghuwanshi N. S. 
Published: March 2021

Abstract
An experimental study was carried out with medium duration rice variety (IR 36) during kharif and rabi seasons of 2015/16 and 2016/17 to investigate the effect of alternate wetting and drying (AWD) practice on water use efficiency, productivity, and consumptive water footprints of rice. The performance of AWD practice was compared with the conventionally (CON) irrigated rice using non-weighing lysimeters. The study resulted that by managing the alternate wetting and drying up to 15 cm below the ground level, a significant reduction in water input (26–29% in kharif and 22–27% in rabi season) could be achieved under AWD. A reduction in evapotranspiration (about 6% in both kharif and rabi seasons) was also observed under AWD. Reduction in consumptive water footprint (about 2–3% in kharif and 2–5% in rabi) was obtained under AWD. Reductions in blue water footprints (7% in kharif and 4–5% in rabi) was also observed under AWD. On average, crop water use efficiency was significantly enhanced by 27–33% and 20–29% in the respective kharif and rabi seasons under AWD practice. Significant improvement in total water productivity by 29–37% and 23–35% in the respective two seasons exhibited the superiority of AWD over CON during the two years of field experiments.
A new approach for suspended sediment load calculation based on generated flow discharge considering climate change
Authors: Adib Arash , Kisi Ozgur , Khoramgah Shekoofeh , Gafouri Hamid Reza , Liaghat Ali , Lotfirad Morteza , Moayyeri Neda 
Published: March 2021

Abstract
Use of general circulation models (GCMs) is common for forecasting of hydrometric and meteorological parameters, but the uncertainty of these models is high. This study developed a new approach for calculation of suspended sediment load (SSL) using historical flow discharge data and SSL data of the Idanak hydrometric station on the Marun River (in the southwest of Iran) from 1968 to 2014. This approach derived sediment rating relation by observed data and determined trend of flow discharge time series data by Mann-Kendall nonparametric trend (MK) test and Theil-Sen approach (TSA). Then, the SSL was calculated for a future period based on forecasted flow discharge data by TSA. Also, one hundred annual and monthly flow discharge time series data (for the duration of 40 years) were generated by the Markov chain and the Monte Carlo (MC) methods and it calculated 90% of total prediction uncertainty bounds for flow discharge time series data by Latin Hypercube Sampling (LHS) on Monte Carlo (MC). It is observed that flow discharge and SSL will increase in summer and will reduce in spring. Also, the annual amount of SSL will reduce from 2,811.15 ton/day to 1,341.25 and 962.05 ton/day in the near and far future, respectively.
Anodic oxidation of bisphenol A by different dimensionally stable electrodes
Authors: Can Orhan T. , Tutun Muhammed M. , Keyikoglu Ramazan 
Published: March 2021

Abstract
Bisphenol A (BPA) is a known endocrine disrupter and was detected in surface waters. We investigated the mineralization of BPA by electrochemical oxidation. Six different types of electrodes, including the boron-doped diamond (BDD), platinum (Pt), and mixed metal oxide (MMO) electrodes; RuO2–IrO2, RuO2–TiO2, IrO2–Ta2O5, and Pt–IrO2, were compared as the anode material. Total organic carbon (TOC) was analyzed to monitor the mineralization efficiency of BPA. BDD achieved 100% BPA mineralization efficiency in 180 min and at a current density of 125 mA/cm2, whereas the TOC removal efficiency of Pt was 60.9% and the efficiency of MMO electrodes ranged between 48 and 54%. BDD exhibited much lower specific energy consumption, which corresponds to a lower energy cost (USD63.4 /kg TOC). The effect of operational parameters showed that the BDD anode was much more affected by the current density, initial BPA concentration, and electrolyte concentration than the other parameters such as the stirring speed and interelectrode distance.
HE4 is a Beneficial Biomarker in Endometrial Adenocarcinoma
Authors: Mohammad Hayam Fathy , El-Khazragy Nashwa , Hassan S. , Abukra Fatma , Abdelrahman Rehab M. , Riad Amr Ahmed Mahmoud , Abdelhafeez Mohamed A. 
Published: March 2021

Background:
Endometrial carcinoma ranks second among the most common gynecological
malignancy in developing countries. Biomarkers are needed to aid in screening the disease and
predicting the clinical as well as the therapeutic outcome. Human Epididymis Protein 4 (HE4) is a
protein that was first isolated from human epididymis cells. HE4 levels are elevated in various
types of cancers, such as ovarian and gastric cancer.

Objectives:
We aim to assess the association of serum and tissue, HE4, and endometrial adenocarcinoma
in 77 Egyptian patients divided into two groups: 37 patients in the endometrial cancer group
and 40 patients in the control group.

Results:
We found that HE4 levels were significantly increased in both serum and tissue of the endometrial
cancer group compared to the negative control group. Moreover, higher expression levels
were significantly associated with high-grade tumors. HE4 showed excellent diagnostic and
prognostic value with a sensitivity and specificity of over 90 percent.

Conclusion:
We conclude that both serum and tissue expression of HE4 are sensitive, specific diagnostic
and prognostic biomarkers in endometrial adenocarcinoma. In addition, they can predict extra-
uterine involvement and staging of the disease.

Clinical Trial Registration No::
PRS-NCT03456791.