bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comparative FISH analysis of Senna tora tandem repeats revealed insights into the chromosome dynamics in Senna
Authors: Ta Thanh Dat , Waminal Nomar Espinosa , Nguyen Thi Hong , Pellerin Remnyl Joyce , Kim Hyun Hee 
Published: March 2021

Abstract
Background
DNA tandem repeats (TRs) are often abundant and occupy discrete regions in eukaryotic genomes. These TRs often cause or generate chromosomal rearrangements, which, in turn, drive chromosome evolution and speciation. Tracing the chromosomal distribution of TRs could therefore provide insights into the chromosome dynamics and speciation among closely related taxa. The basic chromosome number in the genus Senna is 2n = 28, but dysploid species like Senna tora have also been observed.

Objective
To understand the dynamics of these TRs and their impact on S. tora dysploidization.

Methods
We performed a comparative fluorescence in situ hybridization (FISH) analysis among nine closely related Senna species and compared the chromosomal distribution of these repeats from a cytotaxonomic perspective by using the ITS1-5.8S-ITS2 sequence to infer phylogenetic relationships.

Results
Of the nine S. tora TRs, two did not show any FISH signal whereas seven TRs showed similar and contrasting patterns to other Senna species. StoTR01_86, which was localized in the pericentromeric regions in all S. tora, but not at the nucleolar organizer region (NOR) site, was colocalized at the NOR site in all species except in S. siamea. StoTR02_7_tel was mostly localized at chromosome termini, but some species had an interstitial telomeric repeat in a few chromosomes. StoTR05_180 was distributed in the subtelomeric region in most species and was highly amplified in the pericentromeric region in some species. StoTR06_159 was either absent or colocalized in the NOR site in some species, and StoIGS_463, which was localized at the NOR site in S. tora, was either absent or localized at the subtelomeric or pericentromeric regions in other species.

Conclusions
These data suggest that TRs play important roles in S. tora dysploidy and suggest the involvement of 45S rDNA intergenic spacers in “carrying” repeats during genome reshuffling.
Estimated cumulative X-ray exposure and additional cancer risk during the evaluation and treatment of scoliosis in children and young people requiring surgery
Authors: Loughenbury P. R. , Gentles S. L. , Murphy E. J. , Tomlinson J. E. , Borse V. H. , Dunsmuir R. A. , Gummerson N. W. , Millner P. A. , Rao A. S. , Rowbotham E. , Khan A. L. 
Published: March 2021

Abstract
Introduction
Clinicians and patients must weigh the benefits of radiological imaging against the risks of radiation exposure in the diagnosis and treatment of scoliosis. This report aims to estimate the cumulative absorbed and equivalent dose of radiation in patients undergoing surgical treatment for scoliosis, and to present this as an estimated risk of cancer compared to background radiation levels.

Methods
Retrospective review of estimated absorbed dose on the Computerised Radiology Information System (CRIS®). Patients undergoing surgical correction of scoliosis (age ≤ 25) from August 2010 to August 2015 investigated. Estimated absorbed dose [milligrays (mGy)] recorded. Pedicle screws inserted using image intensification. Equivalent dose [millisieverts (mSv)] and additional cancer risk calculated from the National Research Council document ‘Health risks from exposure to low levels of ionising radiation’ (2006).

Results
271 patients identified. Mean age 15 (range 2–25). Mean total absorbed dose 2136 mGy [standard deviation (SD) 1700 mGy]. Mean number of plain spine radiographs was 8 (SD 3) with total 1884 mGy exposure (SD 1609 mGy). Additional dose provided by CT (mean 0.17 episodes), plain chest and abdominal radiographs and image intensification. Mean number of image intensification episodes was 1.1 with mean estimated exposure 180 mGy (SD 238 mGy). Image intensification accounted for 8% of the estimated absorbed dose during treatment. Estimated mean effective dose delivered was 20.952 mSv equating to an additional cancer risk of 0.27–0.45%.

Conclusion
Additional cancer risk from cumulative imaging is small and equivalent to approximately 8 years of natural background radiation. Use of image intensification for pedicle screw insertion is a minor contribution (8%) to the total patient dose.
“The Time of Lament”: A Momand drama of 1711 through the eyes of Pashtun litterateurs
Authors: Pelevin Mikhail 
Published: March 2021

AbstractThis article offers a comparative examination of the literary responses of four leading early modern Pashtun authors to an armed clash in the Momand tribe in 1711. The responses include a chronicle record in prose (Afżal Khān Khaṫak) and three poems – an elegy (ʿAbd al-Raḥmān Momand), a satire (ʿAbd al-Ḥamīd Momand), and a war ode (ʿAbd al-Qādir Khaṫak). Discussed as both authentic historical documents and creative writings linked to a local social discourse, these Pashto texts enable us to reassess the intensity of everyday literary communications in Pashtun tribal areas in early modern times and append new factual material to the study of ethno-cultural processes within the Persophone oecumene. The salient stylistic and rhetoric diversity of the texts not only highlights the authors’ individual mindsets and literary techniques, but also provides an insight into a variety of social moods, political attitudes and ethics in the Pashtun traditional society.
Rice safety and control of penoxsulam-resistant and -susceptible barnyardgrass (Echinochloa crus-galli) populations with soil-applied herbicides
Authors: Liu Tingting , Yu Jialin , Fang Jiapeng , Dong Liyao 
Published: March 2021

AbstractResistance to penoxsulam among barnyardgrass populations is prevalent in rice fields in China. Seeds of penoxsulam-resistant (AXXZ-2) and penoxsulam-susceptible (JLGY-3) barnyardgrass populations, as well as the seeds of two rice varieties, including Wuyungeng32 (WY) and Liangyou669 (LY), were planted in plastic pots and then treated with a rate titration of acetochlor, anilofos, butachlor, clomazone, oxadiazon, pendimethalin, pretilachlor, pyraclonil, or thiobencarb. The two barnyardgrass populations exhibited similar susceptibility to acetochlor, anilofos, butachlor, oxadiazon, pretilachlor, or pyraclonil. However, the susceptibility differed between the barnyardgrass populations in response to clomazone, pendimethalin, and thiobencarb. For AXXZ-2, herbicide rates that caused 50% reduction in shoot biomass from the nontreated control (GR50) were 179, >800, and 1,798 g ha−1 for clomazone, pendimethalin, and thiobencarb, respectively; whereas JLGY-3 GR50 values were 61, 166, and 552 g ha−1, respectively. Both rice varieties demonstrated excellent tolerance to acetochlor, butachlor, oxadiazon, pretilachlor, and thiobencarb. However, substantial rice damage was observed when anilofos and clomazone were used. Anilofos at 352 g ha−1 and clomazone at 448 g ha−1 reduced rice shoot biomass by 41% and 50% from the nontreated, respectively. Averaged across herbicide rates, clomazone use resulted in a reduction in rice shoot biomass from that of the nontreated control by 52% and 34% for WY and LY, respectively; and pendimethalin use resulted in a reduction in rice shoot biomass from the nontreated control by 25% and 9% for WY and LY, respectively.
Cranberry response to sulfentrazone rate, timing, and application volume
Authors: Besançon Thierry E. , Ghantous Katherine M. , Sandler Hilary A. 
Published: March 2021

AbstractThe recent registration of sulfentrazone, a selective, soil-applied, PRE herbicide labeled for control of various weeds in cranberry, expanded the number of modes of action that could be used in the crop. A 2018 preliminary study in Massachusetts showed that high rates of sulfentrazone applied at the cabbage head stage reduced the number of flowering uprights (vertical stems) without impacting the final yield. To clarify the use patterns needed to promote crop safety when using sulfentrazone, six studies were conducted in New Jersey and Massachusetts in 2019 and 2020. Studies compared sulfentrazone applications made at two timings (spring dormant, SD; or cabbage head [CH] stage), two rates (280 and 420 g ai ha−1), and three application volumes simulating either chemigation (3,740 L ha−1) or boom application (190 L ha−1 alone or followed by 0.25 cm water wash-off). Boom application studies in New Jersey in 2018 and 2019 did not show significant long-lasting injury (necrosis or stunting). However, a comprehensive observation of cranberry uprights 8 wk after treatment showed a high rate of terminal bud necrosis, a reduction in the number of reproductive structures, and the development of axillary shoots associated with a high rate of sulfentrazone applied at CH. A mitigation study conducted in 2019 and 2020 confirmed the safety of chemigated sulfentrazone at the high rate with no yield reduction, regardless of crop stage at application. Washing off the herbicide from the cranberry canopy immediately after boom application did prevent the necrosis of terminal bud and the related development of nonproductive secondary shoots. Considering the results of this study, application of sulfentrazone over the top of cranberry vine before scales of the terminal bud start loosening would be prudent practice at this time.
Optical spin-symmetry breaking for high-efficiency directional helicity-multiplexed metaholograms
Authors: Naveed Muhammad Ashar , Ansari Muhammad Afnan , Kim Inki , Badloe Trevon , Kim Joohoon , Oh Dong Kyo , Riaz Kashif , Tauqeer Tauseef , Younis Usman , Saleem Murtaza , Anwar Muhammad Sabieh , Zubair Muhammad , Mehmood Muhammad Qasim , Rho Junsuk 
Published: March 2021

AbstractHelicity-multiplexed metasurfaces based on symmetric spin–orbit interactions (SOIs) have practical limits because they cannot provide central-symmetric holographic imaging. Asymmetric SOIs can effectively address such limitations, with several exciting applications in various fields ranging from asymmetric data inscription in communications to dual side displays in smart mobile devices. Low-loss dielectric materials provide an excellent platform for realizing such exotic phenomena efficiently. In this paper, we demonstrate an asymmetric SOI-dependent transmission-type metasurface in the visible domain using hydrogenated amorphous silicon (a-Si:H) nanoresonators. The proposed design approach is equipped with an additional degree of freedom in designing bi-directional helicity-multiplexed metasurfaces by breaking the conventional limit imposed by the symmetric SOI in half employment of metasurfaces for one circular handedness. Two on-axis, distinct wavefronts are produced with high transmission efficiencies, demonstrating the concept of asymmetric wavefront generation in two antiparallel directions. Additionally, the CMOS compatibility of a-Si:H makes it a cost-effective alternative to gallium nitride (GaN) and titanium dioxide (TiO2) for visible light. The cost-effective fabrication and simplicity of the proposed design technique provide an excellent candidate for high-efficiency, multifunctional, and chip-integrated demonstration of various phenomena.
A novel combination therapy targeting ubiquitin-specific protease 5 in MYCN-driven neuroblastoma
Authors: Cheung Belamy B. , Kleynhans Ane , Mittra Rituparna , Kim Patrick Y. , Holien Jessica K. , Nagy Zsuzsanna , Ciampa Olivia C. , Seneviratne Janith A. , Mayoh Chelsea , Raipuria Mukesh , Gadde Satyanarayana , Massudi Hassina , Wong Iris Poh Ling , Tan Owen , Gong Andrew , Suryano Aldwin , Diakiw Sonya M. , Liu Bing , Arndt Greg M. , Liu Tao , Kumar Naresh , Sangfelt Olle , Zhu Shizhen , Norris Murray D. , Haber Michelle , Carter Daniel R. , Parker Michael W. , Marshall Glenn M. 
Published: March 2021

AbstractHistone deacetylase (HDAC) inhibitors are effective in MYCN-driven cancers, because of a unique need for HDAC recruitment by the MYCN oncogenic signal. However, HDAC inhibitors are much more effective in combination with other anti-cancer agents. To identify novel compounds which act synergistically with HDAC inhibitor, such as suberanoyl hydroxamic acid (SAHA), we performed a cell-based, high-throughput drug screen of 10,560 small molecule compounds from a drug-like diversity library and identified a small molecule compound (SE486-11) which synergistically enhanced the cytotoxic effects of SAHA. Effects of drug combinations on cell viability, proliferation, apoptosis and colony forming were assessed in a panel of neuroblastoma cell lines. Treatment with SAHA and SE486-11 increased MYCN ubiquitination and degradation, and markedly inhibited tumorigenesis in neuroblastoma xenografts, and, MYCN transgenic zebrafish and mice. The combination reduced ubiquitin-specific protease 5 (USP5) levels and increased unanchored polyubiquitin chains. Overexpression of USP5 rescued neuroblastoma cells from the cytopathic effects of the combination and reduced unanchored polyubiquitin, suggesting USP5 is a therapeutic target of the combination. SAHA and SE486-11 directly bound to USP5 and the drug combination exhibited a 100-fold higher binding to USP5 than individual drugs alone in microscale thermophoresis assays. MYCN bound to the USP5 promoter and induced USP5 gene expression suggesting that USP5 and MYCN expression created a forward positive feedback loop in neuroblastoma cells. Thus, USP5 acts as an oncogenic cofactor with MYCN in neuroblastoma and the novel combination of HDAC inhibitor with SE486-11 represents a novel therapeutic approach for the treatment of MYCN-driven neuroblastoma.
Single-cell RNA sequencing reveals that targeting HSP90 suppresses PDAC progression by restraining mitochondrial bioenergetics
Authors: Hu Li-Peng , Zhou Kai-Xia , Huo Yan-Miao , Liu De-Jun , Li Qing , Yang Min-Wei , Huang Pei-Qi , Xu Chun-Jie , Tian Guang-Ang , Yao Lin-Li , Zhang Xue-Li , Wang Ya-Hui , Li Jun , Zhang Zhi-Gang , Jiang Shu-Heng , Xing Xin , Wang Xu , Qin Wei-Ting , Yang Qin 
Published: March 2021

AbstractPancreatic ductal adenocarcinoma (PDAC) is one of the deadliest cancers, which lacks effective treatment strategies. There is an urgent need for the development of new strategies for PDAC therapy. The genetic and phenotypic heterogeneity of PDAC cancer cell populations poses further challenges in the clinical management of PDAC. In this study, we performed single-cell RNA sequencing to characterize PDAC tumors from KPC mice. Functional studies and clinical analysis showed that PDAC cluster 2 cells with highly Hsp90 expression is much more aggressive than the other clusters. Genetic and pharmacologic inhibition of Hsp90 impaired tumor cell growth both in vitro and in vivo. Further mechanistic study revealed that HSP90 inhibition disrupted the interaction between HSP90 and OPA1, leading to a reduction in mitochondrial cristae amount and mitochondrial energy production. Collectively, our study reveals that HSP90 might be a potential therapeutic target for PDAC.
Mammillary body atrophy and other MRI correlates of school-age outcome following neonatal hypoxic-ischemic encephalopathy
Authors: Annink Kim V. , de Vries Linda S. , Groenendaal Floris , Eijsermans Rian M. J. C. , Mocking Manouk , van Schooneveld Monique M. J. , Dudink Jeroen , van Straaten Henrica L. M. , Benders Manon J. N. L. , Lequin Maarten , van der Aa Niek E. 
Published: March 2021

AbstractThe mammillary bodies (MB) and hippocampi are important for memory function and are often affected following neonatal hypoxic ischemic encephalopathy (HIE). The aim of this study was to assess neurodevelopmental outcome in 10-year-old children with HIE with and without therapeutic hypothermia. Additional aims were to assess the associations between MB atrophy, brain volumes (including the hippocampi), white matter microstructure and neurodevelopmental outcome at school-age. Ten-year-old children with HIE were included, who were treated with therapeutic hypothermia (n = 22) or would have qualified but were born before this became standard of care (n = 28). Children completed a neuropsychological and motor assessment and MRI. Mammillary bodies were scored as normal or atrophic at 10 years. Brain volumes were segmented on childhood MRI and DTI scans were analysed using tract-based spatial statistics. Children with HIE suffered from neurocognitive and memory problems at school-age, irrespective of hypothermia. Hippocampal volumes and MB atrophy were associated with total and performance IQ, processing speed and episodic memory in both groups. Normal MB and larger hippocampi were positively associated with global fractional anisotropy. In conclusion, injury to the MB and hippocampi was associated with neurocognition and memory at school-age in HIE and might be an early biomarker for neurocognitive and memory problems.
The analysis of GSTA1 promoter genetic and functional diversity of human populations
Authors: Mlakar Vid , Curtis Patricia Huezo-Diaz , Armengol Marc , Ythier Victor , Dupanloup Isabelle , Hassine Khalil Ben , Lesne Laurence , Murr Rabih , Mlakar Simona Jurkovic , Nava Tiago , Ansari Marc 
Published: March 2021

AbstractGSTA1 encodes a member of a family of enzymes that function to add glutathione to target electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins, and products of oxidative stress. GSTA1 has several functional SNPs within its promoter region that are responsible for a change in its expression by altering promoter function. This study aims to investigate distributions of GSTA1 promoter haplotypes across different human populations and to assess their impact on the expression of GSTA1. PHASE 2.1.1 was used to infer haplotypes and diplotypes of six GSTA1 promoter SNPs on 2501 individuals from 26 populations classified by the 1000 Genomes Project into five super-populations that included Africa (N = 660), America (N = 347), East Asia (N = 504), Europe (N = 502), and South Asia (N = 488). We used pairwise FST analysis to compare sub-populations and luciferase reporter assay (LRA) to evaluate the impact of each SNP on activation of transcription and interaction with other SNPs. The distributions of GSTA1 promoter haplotypes and diplotypes were significantly different among the different human populations. Three new promoter haplotypes were found in the African super-population. LRA demonstrated that SNPs at -52 and -69 has the most impact on GSTA1 expression, however other SNPs have a significant impact on transcriptional activity. Based on LRA, a new model of cis-elements interaction is presented. Due to the significant differences in GSTA1 diplotype population frequencies, future pharmacogenomics or disease-related studies would benefit from the inclusion of the complete GSTA1 promoter haplotype based on the newly proposed metabolic grouping derived from the LRA results.
Accurate surface ultraviolet radiation forecasting for clinical applications with deep neural network
Authors: Raksasat R. , Sri-iesaranusorn P. , Pemcharoen J. , Laiwarin P. , Buntoung S. , Janjai S. , Boontaveeyuwat E. , Asawanonda P. , Sriswasdi S. , Chuangsuwanich E. 
Published: March 2021

AbstractExposure to appropriate doses of UV radiation provides enormously health and medical treatment benefits including psoriasis. Typical hospital-based phototherapy cabinets contain a bunch of artificial lamps, either broad-band (main emission spectrum 280–360 nm, maximum 320 nm), or narrow-band UV B irradiation (main emission spectrum 310–315 nm, maximum 311 nm). For patients who cannot access phototherapy centers, sunbathing, or heliotherapy, can be a safe and effective treatment alternative. However, as sunlight contains the full range of UV radiation (290–400 nm), careful sunbathing supervised by photodermatologist based on accurate UV radiation forecast is vital to minimize potential adverse effects. Here, using 10-year UV radiation data collected at Nakhon Pathom, Thailand, we developed a deep learning model for UV radiation prediction which achieves around 10% error for 24-h forecast and 13–16% error for 7-day up to 4-week forecast. Our approach can be extended to UV data from different geographical regions as well as various biological action spectra. This will become one of the key tools for developing national heliotherapy protocol in Thailand. Our model has been made available athttps://github.com/cmb-chula/SurfUVNet.
Expression of inflammatory interleukins and selected miRNAs in non-small cell lung cancer
Authors: Dutkowska Agata , Szmyd Bartosz , Kaszkowiak Marcin , Domańska-Senderowska Daria , Pastuszak-Lewandoska Dorota , Brzeziańska-Lasota Ewa , Kordiak Jacek , Antczak Adam 
Published: March 2021

AbstractTumours are characterised by an ability to avoid immune destruction and the presence of cancer-associated inflammation. Better understanding of the link between lung cancer and such inflammation is vital for early detection and personalized treatment. Thus, we examined the mRNA expression of interleukins IL-1β, IL-6, IL-17 and miR-9, miR-122 as potential useful biomarkers of NSCLC. Tumour tissues, non-cancerous tissue and blood samples were collected from 39 patients with primary NSCLC undergoing surgical treatment. The selected RNA was isolated from tissue samples and selected miRNAs from peripheral blood exosomes. This RNA was transcribed to cDNA and quantified using RT-qPCR. Significantly higher expression of the selected interleukins was observed in non-cancerous than tumour tissue, and IL-6 was significantly higher in the tumour tissue of patients with a history of ≤ 40 pack-years (PYs) (2.197, IQR: 0.821–4.415) than in those with > 40 PYs (0.461, IQR: 0.372–0.741; p = 0.037). It is clear that inflammatory processes play a role in NSCLC, as indicated by the upregulation of IL-1β and IL-6 in tumour and adjacent tissue, and that smoking has a strong influence on inflammation in tumourigenesis, demonstrated by the upregulation of IL-6 in tumour samples among patients with ≤ 40 PYs compared to > 40 PYs.
Electrode and electrolyte configurations for low frequency motion energy harvesting based on reverse electrowetting
Authors: Adhikari Pashupati R. , Tasneem Nishat T. , Reid Russell C. , Mahbub Ifana 
Published: March 2021

AbstractIncreasing demand for self-powered wearable sensors has spurred an urgent need to develop energy harvesting systems that can reliably and sufficiently power these devices. Within the last decade, reverse electrowetting-on-dielectric (REWOD)-based mechanical motion energy harvesting has been developed, where an electrolyte is modulated (repeatedly squeezed) between two dissimilar electrodes under an externally applied mechanical force to generate an AC current. In this work, we explored various combinations of electrolyte concentrations, dielectrics, and dielectric thicknesses to generate maximum output power employing REWOD energy harvester. With the objective of implementing a fully self-powered wearable sensor, a “zero applied-bias-voltage” approach was adopted. Three different concentrations of sodium chloride aqueous solutions (NaCl-0.1 M, NaCl-0.5 M, and NaCl-1.0 M) were used as electrolytes. Likewise, electrodes were fabricated with three different dielectric thicknesses (100 nm, 150 nm, and 200 nm) of Al2O3 and SiO2 with an additional layer of CYTOP for surface hydrophobicity. The REWOD energy harvester and its electrode–electrolyte layers were modeled using lumped components that include a resistor, a capacitor, and a current source representing the harvester. Without using any external bias voltage, AC current generation with a power density of 53.3 nW/cm2 was demonstrated at an external excitation frequency of 3 Hz with an optimal external load. The experimental results were analytically verified using the derived theoretical model. Superior performance of the harvester in terms of the figure-of-merit comparing previously reported works is demonstrated. The novelty of this work lies in the combination of an analytical modeling method and experimental validation that together can be used to increase the REWOD harvested power extensively without requiring any external bias voltage.