bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cross Tolerance to Drought and low Phosphorus Stress in Mungbean is Regulated by Improved Antioxidant Capacity, Biological N2-Fixation, and Differential Transcript Accumulation
Authors: Meena Surendra Kumar , Pandey Renu , Sharma Sandeep , Gayacharan  , Vengavasi Krishnapriya , Dikshit Harsh Kumar , Siddique Kadambot H.M. , Singh Madan Pal 
Published: February 2021

Abstract
Aims The mobility of phosphorus (P) depends on availability of water in soil; both are limited resources for crop production. We studied the mechanisms governing cross tolerance in the contrasting mungbean accessions for drought and low P stress. Methods Tolerant (IC333090 and IC507340) and sensitive (IC488526 and EC397142) mungbean accessions were grown in soil with treatments: control (sufficient P, irrigated), low P (no P, irrigated), drought (sufficient P, irrigation withheld), and combined stress (no P, irrigation withheld) as well as recovery. Results Drought reduced the relative water content and membrane stability index, affecting overall plant growth. Combined stress (low P and drought) significantly increased root growth, leaf area, and biomass in tolerant accessions, which was attributed to enhanced nutrient uptake and symbiotic N2-fixation. Combined stress also increased osmolyte concentration, antioxidative compounds, and scavenging activity of antioxidant enzymes in tolerant accessions while recovery from drought significantly reduced osmolyte concentration. Transcript abundance of candidate genes related to drought and low P was significantly higher in leaves of IC333090 than IC488526. Conversely, low-P-induced genes (VrSPX1, VrPHO1, VrSQD1, VrPEPCase, and VrMDH) in IC488526 were either downregulated or did not significantly change under combined stress. The drought recovery was better in IC333090 due to enhanced expression of stress-responsive genes. Conclusions Tolerant mungbean accession could be used as potential donor parents in breeding programs. Traits imparting cross tolerance to drought and low P stress may facilitate better varietal selection for increased crop productivity under low P, drought, and the combined stress.
Increased STIP1 and Hsp90 Correlate with Progression and Prognosis of Lung Adenocarcinoma
Authors: Rong Biaoxue , Zhang Youwen , Wang Junye , Ye Shucheng , Guo Maoqing , Jiang Shenghua 
Published: February 2021

Abstract
Background: Stress-inducible phosphoprotein 1 (STIP1) and heat shock protein 90 (Hsp90) have been found to be correlated with malignant tumors. The aim of this investigation was to study the relationship between their expressions and lung adenocarcinoma (LAC). Methods: The expressions of STIP1and Hsp90 in LAC cells and tissues were tested by immunohistochemistry and western blot; the correlation between their expressions and clinicopathological parameters of LAC was analyzed by survival analysisand multiple regression analysis. Results: Expressions of STIP1 and Hsp90 were higher in A549 cells and LAC tissues than that in 16 human bronchial epithelial cells (16HBE cells) (P<0.05) and adjacent normal lung tissues (P < 0.05). The expression of STIP1 and Hsp90 in LAC showed a strong positive correlation (P < 0.05) and significantly correlated with lymph node metastasis (P < 0.05), advanced clinical stage (P < 0.05) and shorter survival (P < 0.05) of LAC. Conclusions: Increased expressions of STIP1 and Hsp90 were closely related to malignant biological behavior of LAC, suggesting that they could be used as potential biomarkers and prognostic indicators for LAC.
Au-Ag Binary Alloys on n-GaAs Substrates and Effect of Work Functions on Schottky Barrier Height
Authors: AKKAYA Abdullah 
Published: February 2021

Abstract
In this study, I investigated the effect of work function (ϕm) of AuxAg1−x (x = 0, 0.22, 0.37, 0.71 and 1) on the Au-Ag/n-GaAs Schottky diode (SD) parameters. Ag, Au and three alloys with different compositions deposited on n-GaAs substrates by thermal evaporation method. Surface morphologies of the samples were investigated by atomic force microscope (AFM). Elementel compositions of Schottky metals were conducted by the energy dispersive X-ray spectroscop (EDX). Current-voltage (I-V) and capacitance-voltage (C-V) measurements performed at room temperature. SD parameters such as barrier height (Φb0), ideality factor (n), series resistance (Rs), and interface state density (Dit) of the SD’s were calculated from the obtained I-V and C-V data. Experimental results showed that all calculated SD parameters depends on alloy composition. Lowest mean barrier height value was found as 0.789 ± 0.022 eV for Au/n-GaAs SDs and the highest value was determined 0.847 ± 0.008 eV for Au0.71Ag0.29/n-GaAs SDs from I-V measurements. Weak dependencies of barrier height to ϕm was exist and gap state parameter (S) determined as 0.0526. S value was close to the Bardeen limit (S = 0), and indicates that Fermi level was strongly pinned in Au-Ag/n-GaAs SDs. Also, main SD parameters like series resistance (Rs), ideality factor (n), reverse bias barrier height (ΦbRB), doping density (Nd) and density of interface states (Dit) were calculated via using different methods from I-V and C-V measurement results. In addition, to determine the leakage current mechanism Poole-Frenkel emission (PFE) and Schottky emission (SE) models applied on reverse bias I-V data.
The Impact of Rarity in NICE's Health Technology Appraisals
Authors: Clarke Sophie , Ellis Michelle , Brownrigg Jack 
Published: February 2021

Abstract
In the absence of a framework designed to evaluate medicines for rare diseases in the UK, most orphan medicines are appraised by the National Institute for Health and Care Excellence (NICE) through the Single Technology Appraisal (STA) process. An analysis of STA appraisals of orphan and non-orphan medicines revealed that orphan medicines were subject to a significantly longer mean time in the NICE process than non-orphan medicines (370 days (n=44) vs 277 days (n=118), p=<0.0001). A higher proportion of orphan STAs required more than one Appraisal Committee Meeting (ACM) vs. non-orphan STAs, and orphan STAs were disadvantaged by worse outcomes with respect to positive recommendations than those orphan medicines assessed by Highly Specialised Technology evaluation (HST). The uncertainties inherent to developing orphan medicines may contribute to these disadvantages. Improved understanding of the challenges in drug development for orphan medicines and clearer guidance for decision makers on navigating uncertainty in the HTA process may promote greater equity in access to medicines across rare and common conditions.
Z-score is a possible predictor of the risk of coronary artery lesion development in patients with Kawasaki disease in Japan
Authors: Suzuki Takayuki , Kakimoto Nobuyuki , Tsuchihashi Tomoya , Suenaga Tomohiro , Takeuchi Takashi , Shibuta Shoichi , Kitano Naomi , Suzuki Hiroyuki 
Published: February 2021

Abstract
ABSTRACT
Risk factors for coronary artery lesion (CAL) development in patients with Kawasaki disease (KD) include male sex, age <12 months, intravenous immunoglobulin (IVIG) resistance, and delayed diagnosis. We aimed to explore the relationship between CAL development and Z-score. We enrolled 281 patients with KD who were treated with our protocol. Echocardiography was performed in three phases: pre-treatment (P1), post-treatment (P2), and 4 weeks after onset (P3). The highest Z-score of the right, left main, left anterior descending, and left circumflex coronary arteries was expressed as Zmax at each phase. P3-Zmax ≥2.5 represented CAL development. Clinical parameters, such as laboratory data and Z-scores, were retrospectively compared between patients with and without CAL development. Sixty-seven patients (23.8%) showed a P1-Zmax ≥2.0, and CAL development occurred in 21 patients (7.5%). Independent risk factors associated with CAL development were P1-Zmax, a ΔZmax (P2-Zmax − P1-Zmax) ≥1, male sex, <12 months of age, and resistant to the first IVIG administration (adjusted odds ratio [95% confidence interval]: 1.98 [1.01–3.92], 4.04 [1.11-14.7], 6.62 [1.33–33.04], 4.71 [1.51–14.68], 5.26 [1.62–17.13], respectively). Using receiver operating characteristic curve analysis, a P1-Zmax ≥1.43 detected CAL development with an area under the curve of 0.64 (sensitivity = 81.0%; specificity = 48.1%).
Conclusions : Our results suggest that P1-Zmax and a ΔZmax (P2-Zmax − P1-Zmax) ≥1 may predict CAL development.
Manufacture Porous Materials by Dabco-Based Lonic Liquid
Authors: Sanaeishoar Haleh , Sabbaghan Maryam , Peimanfar Sahar 
Published: February 2021

Abstract
The long-chain ionic liquid (IL) hexadecyl-4-aza-1-azoniabicyclo[2.2.2]octane bromide was used as a template to prepare the hexagonally ordered siliceous mesoporous molecular sieve MCM-41 as well as the disordered mesoporous molecular sieve designated as KIT-1. The synthesized products were studied via X-ray diffraction (XRD), Fourier transform infrared (FTIR) and N2 adsorption-desorption analysis. The surface area (BET), pore volume, and pore diameter (BJH) are determined. These kind of ILs that have DABCO (1,4-diazabicyclo[2.2.2]octane) in their structures, were prepared with an easy method. When the two templates, cetyltrimethylammonium bromide (CTAB) and IL, have the similar structures, MCM-41 mesoporous molecular sieve produced with more ordered, uniform mesoporous channel and high surface area in comparison to without IL. When just IL used instead of CTAB, the KIT-1 with non-uniform mesoporous was obtained. Here we prepared the KIT-1 mesoporous molecular sieve with IL without CTAB but it seems, using dual template with changing IL structures in fabricated of molecular sieve provide new opportunity to design targeted adsorbents and catalysis.
Development and validation of an immune-related prognostic signature in cervical cancer
Authors: Su Rongjia , Jin Chengwen , Bu Hualei , Wang Xiaoyun , Kuang Menghua , Xiang Jiangdong , Jin Chengjuan 
Published: February 2021

Abstract
Background

Cervical cancer is the fourth most frequently gynecological malignancy across the world. Immunotherapies have proved to improve prognosis of cervical cancer. However, few studies on immune-related prognostic signature had been reported in cervical cancer.
Methods

Raw data and clinical information of cervical cancer samples were download from TCGA and UCSC Xena website. Immunophenoscore of immune infiltration cells in cervical cancer samples was calculated through ssGSEA method using GSVA package. WGCNA, Cox regression analysis, LASSO analysis and GSEA analysis were performed to classify cervical cancer prognosis and explore the biological signaling pathway.
Results

There were 8 immune infiltration cells associated with prognosis of cervical cancer. Through WGCNA, 153 genes from 402 immune-related genes were significantly correlated with prognosis of cervical cancer. A 15-gene signature demonstrated powerful predictive ability in prognosis of cervical cancer. GSEA analysis showed multiple signaling pathways containing PD-L1 expression and PD-1 checkpoint pathway differences between high risk and low risk groups. Furthermore, the 15-gene signature was associated with multiple immune cells and immune infiltration in tumor microenvironment.
Conclusion

The 15-gene signature is an effective potential prognostic classifier in the immunotherapies and surveillance of cervical cancer.
A Potent Peroxidase from Solid Cell Culture of Ocimum Basilicum with High Sensitivity for Blood Glucose Determination
Authors: Mohammadnejad Parvin , Asl Saeed Soleimani , Rasoulian Zahra , Aminzadeh Saeed , Ghashghaie Jaleh , Haghbeen Kamahldin 
Published: February 2021

Abstract
Extensive applications of peroxidase (POX) have raised the global market demand at a considerable rate during the forecast period of 2020 - 2025. Nonetheless, the large-scale POX preparation still relies on the extraction from agricultural products, while there is an accumulative driving force toward employing biotechnological processes with agricultural hassle free identity. In pursuit of this trend, a novel heme peroxidase was purified to homogeneity (MW of 40 kD) from the callus culture of Ocimum basilicum L. in darkness on Murashige-Skoog medium supplemented by 2,4-dichlorophenoxyacetic acid (10-6 M) and kinetin (10-5 M). The highest activity of the purified peroxidase (ObPOX) was observed in Tris-base buffer at pH 7.5 and 80 °C. ObPOX showed high stability over pH(s) 5 to 7.5 and temperatures of 15 to 60 °C. ObPOX specific activity was 1245.142 AU mg-1 in the presence of phenol, 4 times higher than that of HRP. ObPOX showed moderate affinity for guaiacol (Km = 21.5 mM), but obtained an exceptionally high specificity constant (kcat/Km = 66743.7 s-1M-1) for GASA (4-[(4-Hydroxy-3-methoxyphenyl) azo]-benzenesulfonic acid), the introduced substrate for determination of blood sugar. Applying ObPOX instead of HRP in glucose measurements of the real samples improved the regression constant of the correlation diagram between the tests and the lab results from 0.958 to 0.981. Physicochemical properties of ObPOX as well as the growth rate of basil callus (5.04 g L-1 per day) and the yield of ObPOX production (35 mg per 100 g dry biomass per subculture) designates O. basilicum cell culture for large-scale production of a robust peroxidase.
Predicting suicidal thoughts and behaviors among college students: a machine learning approach
Authors: Macalli Melissa , Navarro Marie , Orri Massimiliano , Tournier Marie , Thiébaut Rodolphe , Côté Sylvana M. , Tzourio Christophe 
Published: February 2021

Abstract
Suicidal thoughts and behaviors are prevalent among college students. Yet little is known about screening tools to identify students at higher risk. We aimed to develop a risk algorithm to identify the main predictors of suicidal thoughts and behaviors among college students within one-year of baseline assessment. We used data collected in 2013–2019 from the French i-Share cohort, a longitudinal population-based study including 5066 volunteer students. To predict suicidal thoughts and behaviours at follow-up, we used random forests models with 70 potential predictors measured at baseline, including sociodemographic and familial characteristics, mental health and substance use. Model performance was measured using the area under the receiver operating curve (AUC), sensitivity, and positive predictive value. At follow-up, 17.4% of girls and 16.8% of boys reported suicidal thoughts and behaviors. The models achieved good predictive performance: AUC, 0.8; sensitivity, 79% for girls, 81% for boys; and positive predictive value, 40% for girls and 36% for boys. Among the 70 potential predictors, four showed the highest predictive power: 12-month suicidal thoughts, trait anxiety, depression symptoms, and self-esteem. We identified a parsimonious set of mental health indicators that accurately predicted one-year suicidal thoughts and behaviors in a community sample of college students.
Microstructural and Creep Characterization of Sn-0.7Cu and Sn-0.7Cu-xBi Lead-free Solders for Low Cost Electronic Applications
Authors: Alsowidy Shakib , Aljarbani Ahmed M. , Gumaan Mohammed S. 
Published: February 2021

Abstract
The microstructural, creep resistance and electrical conductivity properties of Sn-0.7Cu eutectic alloy with Bi-additions in concentrations (0.3 and 0.5 wt.%) have been investigated using x-ray diffraction (XRD), Scanning electron microscope (SEM), creep testing machine and (DC) circuit respectively. The three samples were prepared from high purity (99.99%) elements Sn, Cu, and Bi using melting technique. XRD and SEM analysis showed that the eutectic Sn-0.7Cu alloy composed primarily of two phases; a body centered tetragonal 𝜷-Sn phase and monoclinic IMC Cu 6 Sn 5 , the additions of 0.3 and 0.5 wt.% Bi to the eutectic Sn-0.7Cu promote the formation of IMC Cu 6 Sn 5. The above motioned Bi additions have refined the 𝜷-Sn particle size. Creep tests showed that the creep strain rate increases and the creep lifetime (rupture time) decreases as the applied stress and temperature rises. Improvements in creep resistance solders have been achieved by increasing the Bi content up to 0.5 wt. %. The mean values of stress exponent and activation energy indicate that the dislocation creep is the dominate controlling mechanism. The electrical conductivity of the samples was calculated at room temperature, it has the highest value with 0.3 wt.% of Bi.
Risk factors and ramifications offailure to achieve cervical ripening with prostaglandins – Retrospective Cohort Study
Authors: Berezowsky Alexandra , Zeevi Gil , Hadar Eran , Krispin Eyal 
Published: February 2021

Abstract
Background Induction of labor (IOL) with prostaglandins is a common obstetric procedure. We aimed to assess baseline characteristics and evaluate the outcomes of women who failed to respond to labor induction.Methods Retrospective cohort analysis of all women with singleton gestation who underwent induction of labor, due to prolonged pregnancy, with a slow release prostaglandin-E2 (PGE2) vaginal insert (PROPESS) for cervical ripening. During the study period (2012–2018), 1285 were analyzed. They were divided into 3 subgroups accordingly: a) responders − 1,202 (93.54%) - achieved ripening; b) partial responders − 39 (3.04%) – achieved partial ripening and proceeded to oxytocin augmentation; and c) non responders − 44 (3.42%) - required a second ripening method with extra amniotic foley catheter balloon (EAB). Baseline characteristics and labor outcomes were compared between the groups. Primary outcome was defined as intrapartum cesarean delivery (CD) rate. Secondary outcomes were defined as composite adverse maternal and composite adverse neonatal outcomes. A multivariant analysis was utilized to adjust outcomes to potential confounders.Results Women in the responders’ group were younger (30.03 vs. 32.13 and 31.32 years, p = 0.005), and had lower rates of primiparity (76.92% and 81.39% vs 50.99%, p < 0.001). Women in the responders group less frequently required intrapartum cesarean delivery (3.49% vs. 33.33% and 36.36%, p < 0.001). Failure to achieve a cervical ripening with PGE2 (both non-responders and partial responders) was found to be an independent risk factor for cesarean delivery following multivariate analysis (aOR 11.90, 95% CI 6.13–23.25).Conclusion Women who fail to achieve cervical ripening with PGE2 are older, and more often primiparous. This group is associated with higher rates of intrapartum cesarean delivery and adverse outcomes.
Fucosidosis in Tunisians patients: Mutational analysis and homology-based modeling of FUCA1 enzyme
Authors: Chkioua Latifa , Amri Yessine , Saheli Chayma , Fenni Ferdawes , Boudabous Hela , Turkia Hadhami Ben , Massoud Taieb , Tebib Neji , Laradi Sandrine 
Published: February 2021

Abstract
Background: Fucosidosis is an autosomal recessive lysosomal storage disease caused by defective alpha-L-fucosidase (FUCA1) activity, leading to the accumulation of fucose-containing glycolipids and glycoproteins in various tissues. Clinical features include angiokeratoma, progressive psychomotor retardation, neurologic signs, coarse facial features, and dysostosis multiplex.Methods: All exons and flanking intron regions of FUCA1 were screened by direct sequencing to identify mutations and polymorphisms in three unrelated families with fucosidosis. Bioinformatics tools were then used to predict the impacts of novel alterations on the structure and function of proteins. Furthermore, the identified mutations were localized onto a 3D structure model usingthe DeepView Swiss-PdbViewer 4.1 software, which established a function-structure relationship of the FUCA1 proteins.Results: Four novel mutations were identified in this study. Two patients (P1 and P2) in Families 1 and 2 who had the severe phenotype were homoallelic for the two identified frameshift mutations p.K57Sfs*75 and p.F77Sfs*55, respectively. The affected patient (P3) from Family 3, who had the milder phenotype, was heterozygous for the novel missense mutation p.G332E and the novel splice site mutation c.662+5g>c. We verified that this sequence variation did not correspond to a polymorphism by testing 50 unrelated individuals. Additionally, 16 FUCA1 polymorphisms were identified. Crystallographic structure analysis revealed that the missense mutation p.G332E would probably lead to a significant conformational change, thereby preventing the expression of the FUCA1 protein. The 3D structural model of the FUCA1 protein reveals that the glycine at position 332is located near a catalytic nucleophilic residue. This makes it likely that the enzymatic function of the protein with p.G332E is severely impaired. Conclusion: These are the first FUCA1 mutations identified in Tunisia that cause the fucosidosis disease. Bioinformatics analysis allowed us to establish an approximate structure-function relationship for the FUCA1 protein, thereby providing better genotype/phenotype correlation knowledge.
Changes in the Management of Urinary Tract Infections in Women: Impact of the New Recommendations on Antibiotic Prescribing Behavior in France, Between 2014 and 2019
Authors: Piraux Arthur , Faure Sébastien , Naber Kurt G. , ALIDJANOV Jakhongir F. , Ramond-Roquin Aline 
Published: February 2021

Abstract
BackgroundUrinary tract infections (UTIs) are among the most common infections that require antibiotic therapy. In December 2015, new guidelines for UTI management were published in France with the aim of reducing antibiotic misuse and the risk of antimicrobial resistance.Objectives To analyze changes in antibiotic prescribing behavior for acute uncomplicated UTI in women in France from 2014 to 2019.MethodsRetrospective study using data extracted from the medico-administrative database 'OpenMedic' that is linked to the French National Health Data System and collects data on the reimbursement of prescribed drugs. The analyses focused on the number of boxes of antibiotics delivered by community pharmacies, the molecule class, and the prescriber's specialty.ResultsOverall, antibiotic dispensing by community pharmacies increased by 8% between 2014 and 2019, but with differences in function of the antibiotic class. The use of antibiotics recommended as first-line and second-line treatment increased (+ 41% for fosfomycin, + 7.53% for pivmecillinam). Conversely, the dispensing of lomefloxacin and norfloxacin decreased by 80%, and that of ciprofloxacin by 23%. Some antibiotics were mostly prescribed by general practitioners (lomefloxacin, pivmecillinam) and others by secondary care physicians (ofloxacin). The dispensing increase was higher for antibiotics prescribed by secondary care physicians than general practitioners (+21% and +5% between 2014 and 2019). ConclusionThese data suggest that the new recommendations are followed, as indicated by the increased prescription of fosfomycin and pivmecillinam and decreased prescription of fluoroquinolones. However, the efficient transmission and implementation of new recommendations by practitioners requires time, means and dedicated tools.