bluuebunny/crossref_metadata_2025_split huggingface dataset:
Erratum: “Breaking the 3/2 barrier for unit distances in three dimensions”
Authors: Sharir Micha , Zahl Joshua 
Published: April 2021

Abstract
We wish to correct an error in the paper “Breaking the 3/2 barrier for unit distances in three dimensions.” Lemma 3.2 of this paper contained an incorrect claim about the intersection patterns of ellipses obtained by projecting circles from three dimensions to the plane. In this erratum we state and prove a correct version of this statement.
Insulating Peace: Managerial Coordination in Durable Security Complexes
Authors: Travlos Konstantinos 
Published: April 2021

AbstractI argue that insulation via managerial coordination is a key element in any explanation about the formation of political regions among states. The key role it plays is as a tool for the maintenance of intra-regional pacific relations in the face of diffusion and contagion processes, resulting from continued security linkages with excluded extra-regional states. In order to explore these dynamics, I propose a new reconceptualization of the concept of managerial coordination based on the basic framework concept mapping tool. This leads to clarity about what managerial coordination does as a dimension of insulation. It also necessitates a revamp of the scale of interstate managerial coordination as a measuring instrument of the intensity of collective intentionality toward insulation among the members of a region. I then map the region concept of durable security complex (DSC) as the scope for the enactment of managerial coordination, based on a review of existing region concepts in the new regionalist literature. I then conduct an ideographic proof-of-concept exercise on three DSCs in the presence or absence of managerial coordination. These are the Scandinavian states, the South Asian regional security complex, and the South American Norther Tier local hierarchy. The exercise provides indicators for a number of theoretical propositions worthy of future evaluation.
The feasibility of implementing antibiotic restrictions for fluoroquinolones and cephalosporins: a mixed-methods study across 15 Veterans Health Administration hospitals
Authors: Livorsi Daniel J , Suda Katie J , Cunningham Goedken Cassie , Hockett Sherlock Stacey , Balkenende Erin , Chasco Emily E , Scherer Aaron M , Goto Michihiko , Perencevich Eli N , Goetz Matthew Bidwell , Reisinger Heather Schacht ,   Veterans Affairs-Centers for Disease Control and Prevention Practice-Based Research Network
Published: April 2021

AbstractIntroductionThe optimal method for implementing hospital-level restrictions for antibiotics that carry a high risk of Clostridioides difficile infection has not been identified. We aimed to explore barriers and facilitators to implementing restrictions for fluoroquinolones and third/fourth-generation cephalosporins.MethodsThis mixed-methods study across a purposeful sample of 15 acute-care, geographically dispersed Veterans Health Administration hospitals included electronic surveys and semi-structured interviews (September 2018 to May 2019). Surveys on stewardship strategies were administered at each hospital and summarized with descriptive statistics. Interviews were performed with 30 antibiotic stewardship programme (ASP) champions across all 15 sites and 19 additional stakeholders at a subset of 5 sites; transcripts were analysed using thematic content analysis.ResultsThe most restricted agent was moxifloxacin, which was restricted at 12 (80%) sites. None of the 15 hospitals restricted ceftriaxone. Interviews identified differing opinions on the feasibility of restricting third/fourth-generation cephalosporins and fluoroquinolones. Some participants felt that restrictions could be implemented in a way that was not burdensome to clinicians and did not interfere with timely antibiotic administration. Others expressed concerns about restricting these agents, particularly through prior approval, given their frequent use, the difficulty of enforcing restrictions and potential unintended consequences of steering clinicians towards non-restricted antibiotics. A variety of stewardship strategies were perceived to be effective at reducing the use of these agents.ConclusionsAcross 15 hospitals, there were differing opinions on the feasibility of implementing antibiotic restrictions for third/fourth-generation cephalosporins and fluoroquinolones. While the perceived barrier to implementing restrictions was frequently high, many hospitals were effectively using restrictions and reported few barriers to their use.
Impact of the 2015 Health Information Technology Certification Edition on Interoperability among Hospitals
Authors: Pylypchuk Yuriy , Barker Wesley , Encinosa William , Searcy Talisha 
Published: April 2021

Abstract

Objective
Most nonfederal acute care hospitals use electronic health records (EHRs) certified by the Office of the National Coordinator for Health Information Technology. In 2015, the Office of the National Coordinator for Health Information Technology finalized the 2015 Health IT Certification Edition and adoption by hospitals began in 2016. We examine the impact of the 2015 Edition on rates of interoperable exchange among nonfederal acute hospitals.

Materials and Methods
The study applies a standard difference-in-differences design and a recently developed fixed effects estimator that relaxes the assumption of treatment effects being constant across groups and time. In the analysis, we identify separate effects of the 2015 Edition for hospitals that switched EHR developers and forecast hospitals’ interoperability over 2015 Edition adoption rates.

Results
The adoption of the 2015 Edition increased hospitals’ rates of interoperable exchange and especially benefited hospitals that switched EHR developers in the post-implementation period. Forecasting results indicate that if all hospitals adopted the 2015 Edition, 53% to 61% of hospitals would engage in interoperable health information exchange compared with the current rate of 46%.

Discussion
Hospitals’ levels of interoperability have been rising over the last few years. Adoption of newer technology improved hospitals’ interoperability and accounts for up to 12% of the rise in interoperability.

Conclusions
Certified technology is one mechanism to ensure providers use recent and safe technologies for interoperable exchange. Adoption of certified EHRs improves the nation’s interoperable exchange; however, it has a clear limited effect. Other mechanisms are necessary for achieving comprehensive interoperable exchange.
Cell engineering for the production of hybrid-type N-glycans in HEK293 cells
Authors: Leng Ji-Xiong , Ren Wei-Wei , Li Yuqing , Yang Ganglong , Gao Xiao-Dong , Fujita Morihisa 
Published: April 2021

Abstract
Glycoprotein therapeutics are among the leading products in the biopharmaceutical industry. The heterogeneity of glycans in therapeutic proteins is an issue for maintaining quality, activity and safety during bioprocessing. In this study, we knocked out genes encoding Golgi α-mannosidase-II, MAN2A1 and MAN2A2 in human embryonic kidney 293 (HEK293) cells, establishing an M2D-KO cell line that can produce recombinant proteins mainly with hybrid-type N-glycans. Furthermore, FUT8, which encodes α1,6-fucosyltransferase, was knocked out in the M2D-KO cell line, establishing a DF-KO cell line that can express noncore fucosylated hybrid-type N-glycans. Two recombinant proteins, lysosomal acid lipase and constant fragment of human IgG1, were expressed in the M2D-KO and DF-KO cell lines. Glycan structural analysis revealed that complex-type N-glycans were removed in both M2D-KO and DF-KO cells. Our results suggest that these cell lines are suitable for the production of therapeutic proteins with hybrid-type N-glycans. Moreover, KO cell lines would be useful as models for researching the mechanism of antimetastatic effects in human tumours by swainsonine treatment.
Coinfection ofIxodes scapularis(Acari: Ixodidae) Nymphs WithBabesiaspp. (Piroplasmida: Babesiidae) andBorrelia burgdorferi(Spirochaetales: Spirochaetaceae) in Wisconsin
Authors: Zembsch T E , Lee X , Bron G M , Bartholomay L C , Paskewitz S M 
Published: April 2021

AbstractBorrelia burgdorferi, the spirochete that causes Lyme disease, is endemic and widespread in Wisconsin. Research in the northeastern United States has revealed a positive association between Babesia microti, the main pathogen that causes babesiosis in humans, and Bo. burgdorferi in humans and in ticks. This study was conducted to examine associations between the disease agents in the Upper midwestern United States. Ixodes scapularis Say nymphs (N = 2,858) collected between 2015 and 2017 from nine locations in Wisconsin were tested for Babesia spp. and Borrelia spp. using real-time PCR. Two species of Babesia were detected; Ba. microti and Babesia odocoilei (a parasite of members of the family Cervidae). Prevalence of infection at the nine locations ranged from 0 to 13% for Ba. microti, 11 to 31% for Bo. burgdorferi sensu stricto, and 5.7 to 26% for Ba. odocoilei. Coinfection of nymphs with Bo. burgdorferi and Ba. odocoilei was detected in eight of the nine locations and significant positive associations were observed in two of the eight locations. The prevalence of nymphal coinfection with both and Bo. burgdorferi and Ba. microti ranged from 0.81 to 6.5%. These two pathogens were significantly positively associated in one of the five locations where both pathogens were detected. In the other four locations, the observed prevalence of coinfection was higher than expected in all but one site-year. Clinics and healthcare providers should be aware of the association between Ba. microti and Bo. burgdorferi pathogens when treating patients who report tick bites.
Vector Specificity of the Relapsing Fever Spirochete Borrelia hermsii (Spirochaetales: Borreliaceae) for the Tick Ornithodoros hermsi (Acari: Argasidae) Involves Persistent Infection of the Salivary Glands
Authors: Schwan Tom G 
Published: April 2021

Abstract
The relapsing fever spirochetes Borrelia hermsii and Borrelia turicatae are each maintained and transmitted in nature by their specific tick vectors, Ornithodoros hermsi Wheeler (Acari: Argasidae) and Ornithodoros turicata (Duges), respectively. The basis for this spirochete and vector specificity is not known, but persistent colonization of spirochetes in the tick’s salivary glands is presumed to be essential for transmission by these long-lived ticks that feed in only minutes on their warm-blooded hosts. To examine this hypothesis further, cohorts of O. hermsi and O. turicata were infected with B. hermsii and examined 7–260 d later for infection in their midgut, salivary glands, and synganglion. While the midgut from all ticks of both species at all time points examined were infected with spirochetes, the salivary glands of only O. hermsi remained persistently infected. The salivary glands of O. turicata were susceptible to an early transient infection. However, no spirochetes were observed in these tissues beyond the first 32 d after acquisition. Ticks of both species were fed on mice 112 d after they acquired spirochetes and only those mice fed upon by O. hermsi became infected. Thus, the vector competency for B. hermsii displayed by O. hermsi but not O. turicata lies, in part, in the persistent infection of the salivary glands of the former but not the latter species of tick. The genetic and biochemical mechanisms supporting this spirochete and vector specificity remain to be identified.
Larval Conditioning and Aging of Sand Fly Rearing Medium Affect Oviposition Site Selection in Phlebotomus papatasi (Diptera: Psychodidae) Sand Flies
Authors: Faw Lindsey R , Raymann Kasie , Romo Bechara Nayma , Wasserberg Gideon 
Published: April 2021

Abstract
Sand fly larvae develop in sheltered humid habitats containing decaying organic matter on which they feed. Previously, we showed that gravid females of Phlebotomus papatasi Scopoli (Diptera: Psychodidae) are attracted to and stimulated to lay eggs on larval rearing medium containing larvae. That study, however, did not control for the possible effect of medium aging. Our goal in this study was to evaluate the effect of larval substrate conditioning on attraction and oviposition responses of Ph. papatasi sand flies while controlling for the effect of substrate aging. Initially, we confirmed that the pretreatment fresh larval food sources (to be used as larval conditioned and unconditioned media) did not differ with respect to their effect on attraction and oviposition responses. The larval conditioned medium was produced by rearing larvae to the second/third-instar stage over 3 wk using the same larval food source. To produce larval unconditioned medium, the same amount of fresh larval food was added to a control rearing cup that did not contain larvae but was aged under identical time and conditions. Two-choice bioassays were conducted to evaluate gravid female’s attraction and oviposition response to larval conditioned and unconditioned media. We found that gravid females were significantly attracted (P &lt; 0.05) to larval conditioned medium when compared with unconditioned medium under the same amount of time and conditions. However, no such difference was found with respect to oviposition response. Both attraction and oviposition responses were significantly increased for larval conditioned and unconditioned media in comparison to the initial fresh larval food source.
Sexual differences and sex ratios of dioecious plants under stressful environments
Authors: Liu Miao , Korpelainen Helena , Li Chunyang 
Published: April 2021

AbstractDioecious plants exhibit sexual dimorphism in both sexual features (reproductive organs) and secondary sex characteristics (vegetative traits). Sexual differences in secondary traits, including morphological, physiological and ecological characters, have been commonly associated with trade-offs between the cost of reproduction and other plant functions. Such trade-offs may be modified by environmental stressors, although there is evidence that sexually dimorphic responses to stress do not always exist in all plant species. When sexual dimorphism exists, sexually different responses appear to depend on the species and stress types. Yet, further studies on dioecious plant species are needed to allow the generalization of stress effects on males and females. Additionally, sexual dimorphism may influence the frequency and distribution of the sexes along environmental gradients, likely causing niche differentiation and spatial segregation of sexes. At the present, the causes and mechanisms governing sex ratio biases are poorly understood. This review aims to discuss sex-specific responses and sex ratio biases occurring under adverse conditions, which will advance our knowledge of sexually dimorphic responses to environmental stressors.
Effects of resource sharing directionality on physiologically integrated clones of the invasive Carpobrotus edulis
Authors: Portela Rubén , Barreiro Rodolfo , Roiloa Sergio R 
Published: April 2021

Abstract

Aims
One of the key traits associated with clonal growth in plants is the capacity for physiological integration, which allows resource sharing between connected ramets within a clonal system. Resource transport is expected to occur following a source–sink relationship: from ramets established in rich patches to ramets growing in poor patches. However, some experiments have shown that acropetal transport (from basal to apical modules) usually exceeds basipetal transport (from apical to basal ramets). In this study, we aimed to determine the resource transport directionality in physiologically integrated modules of the invader Carpobrotus edulis.

Methods
We conducted two manipulative experiments under common garden conditions that studied the effect of different nutrient levels located at different positions (basal, medial and apical) on connected and disconnected clonal systems of C. edulis. We compared the biomass partitioning patterns and final biomass of ramets to elucidate whether the effect of physiological integration is affected by the directionality of the resource transport.

Important Findings
Results indicate a prevalent acropetal transport of resources in C. edulis, with a developmentally programmed division of labor where basal ramets were specialized in obtaining soil-based resources and apical ramets specialized in aboveground growth. This biomass partitioning pattern was not affected by the nutrient conditions in which basal or apical ramets were growing, although the highest benefit was achieved by apical ramets growing under the most stressed conditions. This developmentally programmed division of labor is expected to increase the lateral growth of C. edulis, and therefore could have meaningful implications for the expansion of this invasive species.
Plus One: Who Decides Who is One’s Significant Other?
Authors: Shakargy Sharon 
Published: April 2021

Abstract
The law respects essential relationships, most notably marriage. Family members receive various benefits to support the family unit and the relationships within it. While the protection of parental relationships is easily understandable, spousal benefits may be questioned. In the western historical tradition, granting benefits exclusively to marital relationships were justified and practical. Nowadays, this seems less obvious. This article suggests that like wedding invitations, the law too should be open to various ‘plus ones’ so it will reflect, respect, and support the reality of life and not base itself on overly-broad, sometimes unjustified, assumptions founded solely on one traditional official relationship (and its modern variations).
D/H in the refractory organics of comet 67P/Churyumov-Gerasimenko measured by Rosetta/COSIMA
Authors: Paquette J A , Fray N , Bardyn A , Engrand C , Alexander C M O'D , Siljeström S , Cottin H , Merouane S , Isnard R , Stenzel O J , Fischer H , Rynö J , Kissel J , Hilchenbach M 
Published: April 2021

ABSTRACT
The D/H ratio is a clue to the origin and evolution of hydrogen-bearing chemical species in Solar system materials. D/H has been observed in the coma of many comets, but most such measurements have been for gaseous water. We present the first in situ measurements of the D/H ratios in the organic refractory component of cometary dust particles collected at very low impact speeds in the coma of comet 67P/Churyumov-Gerasimenko (hereafter 67P) by the COSIMA instrument onboard Rosetta. The values measured by COSIMA are spatial averages over an approximately 35 × 50 µm2 area. The average D/H ratio for the 25 measured particles is (1.57 ± 0.54) × 10−3, about an order of magnitude higher than the Vienna Standard Mean Ocean Water (VSMOW), but more than an order of magnitude lower than the values measured in gas-phase organics in solar-like protostellar regions and hot cores. This relatively high averaged value suggests that refractory carbonaceous matter in comet 67P is less processed than the most primitive insoluble organic matter (IOM) in meteorites, which has a D/H ratio in the range of about 1 to 7 × 10−4. The cometary particles measured in situ also have a higher H/C ratio than the IOM. We deduce that the measured D/H in cometary refractory organics is an inheritance from the presolar molecular cloud from which the Solar system formed. The high D/H ratios observed in the cometary particles challenges models in which high D/H ratios result solely from processes that operated in the protosolar disc.
3D simulations of photochemical hazes in the atmosphere of hot Jupiter HD 189733b
Authors: Steinrueck Maria E , Showman Adam P , Lavvas Panayotis , Koskinen Tommi , Tan Xianyu , Zhang Xi 
Published: April 2021

ABSTRACT
Photochemical hazes have been suggested as candidate for the high-altitude aerosols observed in the transmission spectra of many hot Jupiters. We present 3D simulations of the hot Jupiter HD 189733b to study how photochemical hazes are transported by atmospheric circulation. The model includes spherical, constant-size haze particles that gravitationally settle and are transported by the winds as passive tracers, with particle radii ranging from 1 nm to 1 $\mu$m. We identify two general types of haze distribution based on particle size: In the small-particle regime (&lt;30 nm), gravitational settling is unimportant, and hazes accumulate in two large mid-latitude vortices centred on the nightside that extend across the morning terminator. Therefore, small hazes are more concentrated at the morning terminator than at the evening terminator. In the large-particle regime (&gt;30 nm), hazes settle out quickly on the nightside, resulting in more hazes at the evening terminator. For small particles, terminator differences in haze mass mixing ratio and temperature considered individually can result in significant differences in the transit spectra of the terminators. When combining both effects for HD 189733b, however, they largely cancel out each other, resulting in very small terminator differences in the spectra. Transit spectra based on the GCM-derived haze distribution fail to reproduce the steep spectral slope at short wavelengths in the current transit observations of HD 189733b. Enhanced sub-grid scale mixing and/or optical properties of hazes differing from soot can explain the mismatch between the model and observations, although uncertainties in temperature and star spots may also contribute to the spectral slope.
Conservation of Aging and Cancer Epigenetic Signatures across Human and Mouse
Authors: Pérez Raúl F , Tejedor Juan Ramón , Santamarina-Ojeda Pablo , Martínez Virginia López , Urdinguio Rocío G , Villamañán Lucía , Candiota Ana Paula , Sarró Noemí Vidal , Barradas Marta , Fernandez-Marcos Pablo Jose , Serrano Manuel , Fernández Agustín F , Fraga Mario F 
Published: April 2021

Abstract
Aging and cancer are two interrelated processes, with aging being a major risk factor for the development of cancer. Parallel epigenetic alterations have been described for both, although differences, especially within the DNA hypomethylation scenario, have also been recently reported. Although many of these observations arise from the use of mouse models, there is a lack of systematic comparisons of human and mouse epigenetic patterns in the context of disease. However, such comparisons are significant as they allow to establish the extent to which some of the observed similarities or differences arise from pre-existing species-specific epigenetic traits. Here, we have used reduced representation bisulfite sequencing to profile the brain methylomes of young and old, tumoral and nontumoral brain samples from human and mouse. We first characterized the baseline epigenomic patterns of the species and subsequently focused on the DNA methylation alterations associated with cancer and aging. Next, we described the functional genomic and epigenomic context associated with the alterations, and finally, we integrated our data to study interspecies DNA methylation levels at orthologous CpG sites. Globally, we found considerable differences between the characteristics of DNA methylation alterations in cancer and aging in both species. Moreover, we describe robust evidence for the conservation of the specific cancer and aging epigenomic signatures in human and mouse. Our observations point toward the preservation of the functional consequences of these alterations at multiple levels of genomic regulation. Finally, our analyses reveal a role for the genomic context in explaining disease- and species-specific epigenetic traits.