bluuebunny/crossref_metadata_2025_split huggingface dataset:
Epigenetic silencing and tumor suppressor gene of HAND2 by targeting ERK signaling in colorectal cancer
Authors: Yuan Zixu , Yu Xihu , Cai Jian , Chen Daici , Liu Xiaoxia , Wu Zhijie , Chen Wenle , Grady William M. , Wang Hui , Chen Chun 
Published: October 2021

Abstract
Background

The screening biomarkers for early detection of colorectal cancer (CRC) is lacking. The aim is to identify epigenetic silenced genes and clarify its roles and underlying mechanism in CRC. We conducted integrative analyses of epigenome-wide Human Methylation 450K arrays and transcriptome to screen out candidate epigenetic driver genes with transcription silencing. Methylated silencing HAND2 were identified and verified in large CRC cohort. The mechanism of HAND2 expression by promoter inhibition were clarified both in vitro and vivo assays. Cell biofunctional roles of HAND2 methylation was investigated in CRC cells. HAND2 reconstitution were constructed by lentivirus plasmid and tumor xenograft model of HAND2 were built subcutaneously. Genomic mRNA analysis by RNA-sequencing and subsequent GSEA analysis were performed to identify potential target of HAND2 and qPCR/WB was conducted to identify the results.
Results

We firstly reported high frequency of HAND2 methylation in promoter in CRC and hypermethylation was negatively correlated with expression silencing and leaded to poor survival in several CRC cohort patients. 5-Aza treatment to demethylated HAND2 could revert its expression in CRC cells. Functionally, HAND2 reconstitution can inhibit cell proliferation, invasion and migration in vitro. In tumor xenograft, HAND2 reconstruction significantly repressed tumor growth when compared to control vector. Thousands of aberrant expressed genes were observed in the heatmap of RNA-sequencing data. HAND2 reconstitution could bind to ERK and reduce its phosphorylation by CoIP assay. These above results showed HAND2 reconstitution perturbed the activation of MAPK/ERK signaling by reduction of ERK phosphorylation.
Conclusions

HAND2 is one tumor suppressor by targeting ERK signaling and one potential epigenetic driver gene in CRC.
The Effect of N-Acetyl Cysteine and Vitamin E on Oxidative Status and Hemoglobin Level in Transfusion-Dependent Thalassemia Patients: A Systematic Review and Meta-Analysis
Authors: Haghpanah Sezaneh , Hosseini-Bensenjan Mahnaz , Zekavat Omid Reza , Bordbar Mohammadreza , Karimi Mehran , Ramzi Mani , Asmarian Naeimehossadat 
Published: October 2021

Abstract
This meta-analysis was conducted to summarize the comparative effect of NAC and Vitamin E on oxidative status, including total antioxidant capacity (TAC), total oxidative stress (TOS), oxidative stress index (OSI), and hemoglobin (Hb) in patients with TDT.All methods were performed in accordance with the PRISMA checklist. We searched databases including Web of Science (ISI), Scopus, PubMed, and Embase. Meta-analysis was done using Stata statistical software version 16.0.Finally,four randomized-controlled trials (RCT)for NAC and four RCTs for Vitamin E were included.Our meta-analyses showed a significant increase in the weighted mean differences (WMD) of Hband a significant decrease in the WMD of OSI in the NAC group.In subgroup analysis based on age: in the NAC group, a significant increase in the WMD of TAC andHband a significant decrease in the WMD of TOS were detectedonly in children. In theVitamin E group, a significant increase in Hb and a significantdecrease in TOS and OSI were determined in the children subgroup as well.NAC can be used as an effective antioxidant supplementparticularlyin children with TDT. Also, Vitamin Eshowed a beneficial antioxidant effect in TDT children. So, more well-designed randomized, controlled trials for the effect of NAC in adult TDT andthe effect of vitamin E both in adults and children are recommended with more focus on theessential influencing factors on the oxidative status in TDT patients.
Clinical Characteristics of Choledochal Cysts With Intrahepatic Bile Duct Dilatations
Authors: Zhang Shuhao , Cai Duote , Zhang Yuebin , Chen Ken , Jin Yi , Luo Wenjuan , Huang Zongwei , Hu Di , Gao Zhigang 
Published: October 2021

Abstract
Background: Whether the dilated intrahepatic bile duct (IHBD) has any effect on prognosis of choledochal cyst (CC) remains controversy. In the study, we aim to summarize the clinical characteristics and prognosis of those patients.Methods: A total of 192 children (47 males, 145 females) diagnosed with CC were identified in our hospital, including 127 without IHBD dilatation (group A) and 65 with IHBD dilatation (group B). A retrospective analysis was performed to explore the clinical characteristics of group B based on clinical indices, symptoms, and complications. Results: Compared with group A, incidences of jaundice and fever were significantly increased in group B (p=0.01 and 0.033, respectively). Preoperative total bilirubin (TB), direct bilirubin (DB), and indirect bilirubin (IDB) were also higher in group B than in group A (p=0.0052, 0.0005, and 0.0136, respectively), as were preoperative levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), gamma-glutamyl transferase (GGT), and total bile acid (TBA) (p=0.0057, 0.0250, 0.0002, and 0.0242, respectively). Early postoperative GGT levels in group B remained abnormal and were significantly higher than in group A (p=0.0003). The risk of liver fibrosis or cirrhosis was significantly increased for group B compared with group A (p=0.012) and also occurred earlier in group B (p<0.001), among them, dilated IHBDs recovered to normal in 89.23% (7/65) of patients. Meanwhile, the incidence of postoperative pancreatitis increased significantly in group B as well (p=0.003).Conclusion: CC with IHBD dilatation was associated with significantly increased preoperative bilirubin levels, abnormal liver function, and higher incidence of liver fibrosis or cirrhosis early in the disease course. Timely radical surgery is recommended for such patients and postoperative extinction of the IHBD dilatation occurs early in most of those patients.
MiR-132-3p suppresses cell proliferation and migration in gastric cancer by targeting KIF21B/Wnt/β-catenin signaling pathway
Authors: Liu Bingtian , Qiang Ling , Guan Bingxin , Ji Zhipeng 
Published: October 2021

Abstract
Background: Recently, kinesin family member 21B (KIF21B) has been reported to be an oncogene in non-small cell lung cancer and hepatocellular carcinoma. However, the functional role and related molecular mechanisms underlying gastric cancer (GC) pathogenesis remain largely uncovered. Methods: The expression of KIF21B was investigated by analysis of Oncomine microarray gene expression datasets and clinical specimens. The association between KIF21B and miR-132-3p was assessed by luciferase reporter assay. CCK-8 assay and transwell assay were performed to analyze the functional role of miR-132-3p/KIF21B in GC cells. Related protein expression levels were evaluated by immunohistochemistry and western blot analysis.Results: We first found that the expression of KIF21B was upregulated in GC tissues compared with adjunct normal tissues. Knockdown of KIF21B significantly suppressed the proliferation, migration and invasion in GC cell lines (AGS and SNU-5). KIF21B was confirmed as the target of miR-132-3p in GC cells. Moreover, miR-132-3p was down-regulated and inversely correlated with KIF21B expression in GC tissues. Further functional experiments demonstrated that overexpression of KIF21B remarkedly reversed the suppressive effects of miR-132-3p overexpression on GC cell proliferation, migration and invasion. Furthermore, miR-132-3p overexpression downregulated the protein levels of Wnt1, c-Myc, β-catenin, PCNA and N-cadherin, and upregulated E-cadherin expression in GC cells, which were all alleviated after KIF21B overexpression. Conclusions: In summary, our findings provide the first evidence that down-regulation of KIF21B by miR-132-3p suppresses cellular functions in GC via regulating Wnt/β-catenin signaling.
Role of Th17 Cytokines in Liver’s Immune Response during Fatal Yellow Fever:Triggering Cell Damage Mechanism
Authors: Carvalho Marcos Luiz Gaia , Sousa Jorge Rodrigues , Lopes Jeferson Costa , Mendes Caio Cesar Henriques , Olímpio Fábio Alves , Silva Carlos Augusto Moreira , Miranda Vanessa Cabral , Santos Lais Carneiro , Vilacoert Fellipe Souza Silva , Azevedo Raimunda do Socorro Silva , Cruz Ana Cecília Ribeiro , Falcão Luiz Fábio Magno , Galúcio Vanessa Costa Alves , Martins Lívia Caricio , Duarte Maria Irma Seixas , Filho Arnaldo Jorge Martins , Vasconcelos Pedro Fernando Costa , Quaresma Juarez Antônio Simões 
Published: October 2021

Abstract
Yellow fever (YF) is an infectious disease whoseevolution and outcome arerelated to the host immune response pattern. We investigated the Th17 cytokine profile in the liver of humans with fatal YF. Liver tissue samples were collected from 26 patients, including 21 YF-positive and five flavivirus-negative patients with preserved hepatic parenchyma architecture who died of other causes. Samples underwent histopathological and immunohistochemical analysis to detect the Th17 profile (ROR-γ, STAT3, IL-6, TGF-β, IL-17,and IL-23). Substantial differences were found in the expression of markers between fatal YF cases and control samples with a predominance of Th17 cytokine markers in the midzonal region of the YF cases, the most affected area in the liver acinus. Histopathological changes in the hepatic parenchyma revealed cellular damage characterised mainly by the presence of inflammatory infiltrate, Councilman bodies (apoptotic cells), micro/macrovesicular steatosis, and lytic and coagulative necrosis.Th17 cytokines play a pivotal role during YF and contribute significantly to triggering the mechanisms of cell damage in the fatal outcome of severe cases.
Impact of Tumor Progression on Survival during Neoadjuvant Chemotherapy in Breast Cancer: A Cohort Study
Authors: Nozawa Kazuki , Takatsuka Daiki , Endo Yuka , Horisawa Nanae , Ozaki Yuri , Kataoka Ayumi , Kotani Haruru , Yoshimura Akiyo , Hattori Masaya , Sawaki Masataka , Iwata Hiroji 
Published: October 2021

Abstract
Background: Although few patients with breast cancer experience tumor progression during neoadjuvant systemic therapy (NST), their extremely poor outcomes are similar to patients who fail to achieve pathological complete response (pCR). No reports have compared patients with pCR, non-pCR, and progression during NST to determine the survival outcomes.Methods: This retrospective chart review of patients with stage I–III breast cancer was conducted between January 2001 and December 2018. pCR was defined as no invasive cancer or in situ residuals in the breast and lymph nodes (ypT0 ypN0). Disease-free survival (DFS) and overall survival (OS) were estimated using Kaplan–Meier methods.Results: Of the 595 patients who received NST, 167 (29.2%) had pCR (pCR group), 404 (70.8%) responded to NST but did not have pCR (non-pCR group), and 24 (4.0%) experienced tumor progression during NST (PD group). The median DFS was 6.0 months, 154.0 months, and not reached in the PD, non-pCR, and pCR groups, respectively. The PD group had significantly shorter DFS than patients without tumor progression in the pCR and non-pCR groups [hazard ratios (HR) 13.0, 95% CI: 8.1–21.0, p < 0.01]. The median OS was 13.6 months (95% CI: 10.4–35.5) in the PD group and was not reached in the pCR and non-pCR (non-PD) groups. The OS was significantly poorer in the PD group than in the non-PD group (HR 15.8, 95% CI: 9.2–27.1, p < 0.01).Conclusion: The PD group had the poorest survival outcomes even after recurrence, thus warranting new treatment targets.
An Image-Guided Network for Depth Edge Enhancement
Authors: Lee Kuan-Ting , Liu En-Rwei , Yang Jar-Ferr , Hong Li 
Published: October 2021

Abstract
With the rapid development of 3D coding and display technologies, numerous applications are emerging to target human immersive entertainments. To achieve a prime 3D visual experience, high accuracy depth maps play a crucial role. However, depth maps retrieved from most devices still suffer inaccuracies at object boundaries. Therefore, a depth enhancement system is usually needed to correct the error. Recent developments by applying deep learning to deep enhancement have shown their promising improvement. In this paper, we propose a deep depth enhancement network system that effectively corrects the inaccurate depth using color images as a guide. The proposed network contains both depth and image branches, where we combine a new set of features from the image branch with those from the depth branch. Experimental results show that the proposed system achieves a better depth correction performance than state of the art advanced networks. The ablation study reveals that the proposed loss functions in use of image information can enhance depth map accuracy effectively.
Earlier Snowmelt May Lead to Late Season Declines in Plant Productivity and Carbon Sequestration in Arctic Tundra Ecosystems
Authors: Zona Donatella , Lafleur Peter , Hufkens Koen , Bailey Barbara , Gioli Beniamino , Burba George , Goodrich Jordan , Liljedahl Anna , Euskirchen Eugenie , Watts Jennifer , Farina Mary , Kimball John , Heimann Martin , Goeckede Mathias , Pallandt Martijn , Christensen Torben , Mastepanov Mikhail , Lopez-Blanco Efren , Jackowicz-Korczynski Marcin , Dolman Albertus J. , Marchesini Luca Belelli , Commane Roisin , Wofsy Steve , Miller Charles , Lipson David , Hashemi Josh , Arndt Kyle , Kutzbach Lars , Holl David , Boike Julia , Wille Christian , Sachs Torsten , Kalhori Aram , Song Xia , Xu Xiaofeng , Humphreys Elyn , Koven Charles , Sonnentag Oliver , Meyer Gesa , Gosselin Gabriel , Marsh Philip , Oechel Walter 
Published: October 2021

Abstract
Arctic warming is affecting snow cover and soil hydrology, with consequences for carbon sequestration in tundra ecosystems. The scarcity of observations in the Arctic has limited our understanding of the impact of covarying environmental drivers on the carbon balance of tundra ecosystems. In this study, we address some of these uncertainties through a novel record of 119 site-years of summer data from eddy covariance towers representing dominant tundra vegetation types located on continuous permafrost in the Arctic.Here we found that earlier snowmelt was associated with more net CO2 sequestration and higher gross primary productivity (GPP) only in June and July, but with lower net carbon sequestration and lower GPP in August. Although higher evapotranspiration (ET) can result in soil drying with the progression of the summer, we did not find significantly lower soil moisture with earlier snowmelt, nor evidence for a water stress that affected GPP in the peak and late growing season. Our results suggest that climate change and the associated increased length in the growing season might not benefit these northern tundra ecosystems if they are not able to continue sequestering CO2 later in the season.
General Practice Clinical Pharmacist Standards of Practice for Delivery of Polypharmacy and Chronic Disease Medication Reviews: A Consensus Study.
Authors: Earle-Payne Katie , Forsyth Paul , Johnson Chris F , Harrison Heather , Robertson Susan , Weidmann Anita E 
Published: October 2021

Abstract
BackgroundGeneral practice in the UK is experiencing a crisis. Greater multidisciplinary working is a potential solution. The new general practice contract in Scotland encourages this and includes a new pharmacotherapy service to be delivered by General Practice Clinical Pharmacists (GPCPs). Consensus is lacking for the standards of practice for delivery of pharmacotherapy medication reviews (which are polypharmacy and chronic medication reviews) as part of this service.AimTo identify and validate standards of practice for polypharmacy and chronic disease medication (pharmacotherapy level 3) reviews conducted by GPCPs. MethodA two-phased mixed-methods consensus methodology was used. Phase 1: An expert group of GPCPs (n=4) and clinical pharmacist managers (n=2) responsible for delivering the pharmacotherapy service used a Modified Nominal Group Technique to generate potential standards. Phase 2: Two-round Delphi survey involving GPCPs with ≥1 year of experience of working in general practice (n=159).ResultsThe expert group identified 44 potential standards of practice for polypharmacy and chronic disease reviews. Practicing GPCPs indicated during the Delphi phase that the 44 standards were applicable to practice. The standards of practice covered seven main categories: skills, environment, qualifications, qualities and behaviours, knowledge, process and experience.ConclusionPracticing GPCPs indicated that the standards identified by the expert group are acceptable and valid for current practice and the delivery of polypharmacy and chronic medication reviews. The application of these standards to practice may help GPCPs and general practices to ensure equitable delivery of patient care.
Increasing Buffering Capacity Enhances Rumen Fermentation Characteristics and Alters Rumen Microbiota Composition of High-Concentrate Fed Hanwoo Steers
Authors: Ramos Sonny C. , Kim Seon Ho , Jeong Chang Dae , Mamuad Lovelia L. , Son A-Rang , Kang Seung Ha , Cho Yong Il , Kim Tae Gyu , Lee Jin Sung , Cho Kwang Keun , Lee Sung Sill , Lee Sang Suk 
Published: October 2021

Abstract
This study determined the buffering capacity of buffer agents and its effect on in vitro and in vivo rumen fermentation characteristics and bacterial composition of a high-concentrate fed Hanwoo steers. Both BC0.9% and BC0.5% had significantly highest buffering capacity, pH, and ammonia-nitrogen (NH3-N) than BC0.3% and CON at 24 h incubation. Individual and total volatile fatty acids (VFA) were significantly lowest in CON. Phylum Bacteroidetes dominated all treatments but a higher abundance of Firmicutes in BC0.5% than others. Ruminoccocus bromii and Succiniclasticum ruminis were dominant in BC0.5% and Bacteroides massiliensis in BC0.3%. The normalized data of relative abundance of observed OTUs’ representative families have grouped the CON with BC0.3% in the same cluster, whereas BC0.5% and BC0.9% were clustered separately which indicates the effect of varying buffering capacity of buffer agents. Principal coordinate analysis (PCoA) on unweighted UniFrac distances revealed close similarity of bacterial community structures within and between treatments and control, in which BC0.9% and BC0.3% groups showed dispersed community distribution. Overall, the increasing buffering capacity enhances rumen fermentation parameters and affects rumen microbiome by altering bacterial community through distinct structure between high and low buffering capacity, thus an important factor to prevent ruminal acidosis during a high-concentrate diet.
Prevention of Postoperative Events Following Reversal With Sugammadex or Neostigmine (The P- PERSoN Trial) – Pilot Data Following Early Termination of a Prospective, Blinded, Randomised Trial.
Authors: Olesnicky Benjamin Luke , Doane Matthew , Farrell Clare , Knoblanche Greg , Delaney Anthony 
Published: October 2021

Abstract
Background: Residual paralysis following anaesthesia is common and can lead to postoperative morbidity. While sugammadex has been shown to be effective in minimising residual paralysis, uncertainty exists as to whether its use reduces any associated morbidity. We designed this trial to determine if the use of sugammadex for the reversal of intraoperative aminosteroid neuromuscular blockade results in improvements in postoperative pulmonary complications, complications in the recovery unit, postoperative nausea and vomiting, and patient satisfaction, when compared to reversal with neostigmine. Methods: A prospective, double-blind, randomised controlled trial in adult patients admitted for surgical operations at two Australian hospitals between December 2018 and March 2019 was performed comparing the reversal of neuromuscular paralysis using sugammadex 2mg/kg versus neostigmine 50mcg/kg. Statistical analysis of continuous data was performed using two tailed t-tests, with categorical and ordinal data was assessed by Chi-squared analysis. Results: The trial was terminated due to a combination of resource constraints and the 2019 novel coronavirus disease (COVID-19) pandemic. Of 51 patients screened, 33 were eligible for participation and 30 subsequently recruited and randomised. All patients received the intended treatment allocated. Data for the primary outcome was obtained in all patients. There was no difference in the rates of postoperative pulmonary complications between the sugammadex and neostigmine groups (0% (0/19) vs 9% (1/11) RR 5.0 (95%CI 0.22-113) p=0.37. There was no difference in any of the secondary outcomes between the groups. Conclusions: The P-PERSoN trial showed no difference in post operative pulmonary complications between sugammadex and neostigmine based reversal of aminosteroid neuromuscular block, but was underpowered to show any difference due to early trial termination. The randomisation and data collection was feasible. We support the need for an adequately resourced and funded randomised controlled trial to address this important clinical question. Trial Registration: The P-PERSoN trial is registered at the Australian and New Zealand Trial Registry (www.anzctr.org.au) # ACTRN12616000063415 and The U.S. National Library of Medicine (www.ClinicalTrials.gov) #NCT02825576
Analysis on factors behind sentinel lymph node metastasis in breast cancer by color ultrasonography, molybdenum target and pathological detection
Authors: Yiming Aibibai , Wubulikasimu Muhetaer , Yusuying Nuermaimaiti 
Published: October 2021

Abstract
Background
This study aimed to collect the factors underlying the metastasis of breast cancer and sentinel lymph node, and to screen and analyze the risk factors of sentinel lymph node metastasis, so as to provide reference and basis for clinical work.
Methods
A total of 100 patients with breast cancer were enrolled in the study. These patients received treatment in our hospital from May 2017 to May 2020. The general information, the characteristics of the color Doppler echocardiography, molybdenum, conventional pathology and molecular pathology of the patients were collected. The influencing factors of sentinel lymph node metastasis in breast cancer patients were retrospectively analyzed.
Results
In this study, age, tumor diameter and BI-RADS category, pathology type, expression profiles of CK5/6, EGFR, and CK19, and TP53 and BRAC1/2 mutations were independent risk factors for sentinel lymph node metastasis in breast cancer (P<0.05). The number and locations of tumors, the quadrant of tumors, the regularity of tumor margins, the presence of blood flow signals, the presence of posterior echo attenuation, the presence of calcification, histological grade, molecular typing, and mutations of BRAF, ATM and PALB2 were irrelevant factors (P>0.05).
Conclusions
In conclusion, age, tumor diameter, Bi-rads category, invasive type, expression of CK5/6, EGFR and CK19, and mutations of TP53 and BRAC1/2 were positively correlated with sentinel lymph node metastasis. They are independent risk factors that should be paid more attention in clinical studies, to strengthen the management and control of sentinel lymph node metastasis of high-risk breast cancer, and support early chemotherapy or targeted therapy.