bluuebunny/crossref_metadata_2025_split huggingface dataset:
Predictive Value of The G1–G6 Transcriptomic Molecular Classification of Hepatocellular Carcinoma for its Biological Behavior and Clinicopathological Features in China
Authors: Chen Ruonan , Zhou Zixiong , He Wubing , Chen Yi , Huang Aimin , Chen Lihong 
Published: October 2021

Abstract
Background: Hepatocellular carcinoma (HCC) is one of the most common cancers worldwide and a large number of genetic alterations are involved in the carcinogenetic process. A G1–G6 transcriptomic classification was previously proposed in a French study, and Korean and Singaporean groups indicated its potential application in Asian HCC patients. However, the genomic profiles of Chinese patients are distinct from patients of other regions, and therefore the suitability of this method in Chinese HCC patients has remained unknown.Materials/Methods: In this study, we tested the transcriptomic group classification from the French cohort on a cohort of HCC patients from China. a total of 107 HCC cases from China were selected for the G1–G6 transcriptomic molecular classification. The correlation between the G1–G6 molecular classification and clinicopathological features were analyzed. RNA sequencing and bioinformatics analysis were performed to screen related targets and molecular signaling pathways.Results: We investigated the G1–G6 signatures in 107 Chinese HCC patients. HCC cases from China (n=107) were distributed as follows: G1 (17.76%), G2 (1.87%), G3 (18.69%), G4 (9.35%), G5 (23.36%), and G6 (28.97%) groups. We observed concordance between the genetic profiles and clinical features of Chinese HCC patients and French HCC patients. We found that the G1–G3 subgroups were associated with high serum alpha-fetoprotein (AFP) level, high copy number of hepatitis B virus (HBV) DNA, complex histopathological structure, macrovascular invasion, negative or weak Hep-Par1 expression, programmed death-ligand 1 expression, and liver cancer stemness. The G1 subgroup was mainly related to liver cancer stemness, and G3 subgroup showed the worst prognosis. The G5 and G6 subgroups were associated with activation of the Wnt/β-catenin pathway. Compared with the G1–G4 group, the G1–G3 group showed significantly higher expression levels of regenerating family member 1 beta (REG1B), regenerating family member 3 gamma (REG3G), and inositol 1,4,5-trisphosphate receptor type 1 (ITPR1), and enriched calcium signaling pathway.Conclusions: Our results clarify the correlation between G1–G6 molecular classification and molecular markers and molecular signaling pathways in the Chinese HCC population and initially established a link between the phenotype and molecular characteristics. This study enhances our understanding of the heterogenicity of China HCC and indicates that the G1–G6 signatures can be used to identify potential therapeutic biomarkers against HCC patients in China.
Effects of Food-Specific IgG on Health Outcomes of Asymptomatic Physical Examination Population
Authors: Wu Mingxia , Wang Xiaofang , Sun Li , Chen Zongtao 
Published: October 2021

Abstract
Background: Although food-specific IgG is associated with the development and progression of some diseases as shown by many studies, it is also present in the population without clinical symptoms. However, the damage of the immune response it evokes to the health of such population has not been studied yet.Methods: The asymptomatic physical examination population (APEP) was selected according to the inclusion and exclusion criteria, and the physical examination data were collected. The subjects were divided into IgG-positive group (IgG-po group) and IgG-negative group (IgG-neg group). The hematologic and imaging examination results were compared between the two groups, and their odds ratios (OR) and 95% confidence interval (95%CI) were calculated using binary logistic regression to determine the relationship between food-specific IgG and different health outcomes. Results: The data of 28,292 subjects were included in the analysis of the study. The overall IgG positive rate was up to 52.3%, with mild to moderate IgG positive predominantly. Compared with IgG negative group, IgG positive group was associated with a decrease in the risks of hypertriglyceridemia, abnormal fasting blood glucose and overweight(OR=0.87,95%:CI:0.83-0.92; OR=0.93,95%CI:0.87-0.99; OR=0.92,95%CI:0.87-0.96) and with an increase in the incidence rate of thyroid nodule (OR=1.09,95%CI:1.04-1.15).Conclusion: Food-specific IgG is generally present in APEP and has an unique distribution profile, which plays a positive role in maintaining normal body weight and metabolic indicators(triglyceride(TG) and fasting blood glucose(FBG)), without influencing the nutritional status. The physiological mechanism is worth further study.
ROS/PI3K/Akt and Wnt/β-Catenin Signalings Activate HIF-1α-Induced Metabolic Reprogramming to Impart 5-Fluorouracil Resistance in Colorectal Cancer
Authors: Dong Shuohui , Liang Shuo , Cheng Zhiqiang , Zhang Xiang , Luo Li , Li Linchuan , Zhang Wenjie , Li Songhan , Xv Qian , Zhong Mingwei , Zhu Jiankang , Zhang Guangyong , Hu Sanyuan 
Published: October 2021

Abstract
Background: Acquired resistance of 5-fluorouracil (5-FU) remains a clinical challenge in colorectal cancer (CRC), and efforts to develop targeted agents to reduce resistance have not yielded success. Metabolic reprogramming is a key cancer hallmark and confers several tumor phenotypes including chemoresistance. Glucose metabolic reprogramming events of 5-FU resistance in CRC has not been evaluated, and whether abnormal glucose metabolism could impart 5-FU resistance in CRC is also poorly defined.Methods: We generated three acquired 5-FU resistance CRC cell line models, and the detailed assessment of glucose metabolism was performed by in vitro and in vivo experiments, including glucose and lactate utilization, the RNA and protein expressions of glucose metabolism‐related enzymes, the changes of intermediate metabolites of glucose metabolite pool and so on. We detected the protein levels of hypoxia inducible factor 1α (HIF-1α) in primary tumors and circulating tumor cells of CRC patients by immunohistochemistry and immunofluorescence. Stably HIF1A knockdown in cell models were established with a lentiviral system. The influence of both HIF1A gene knockdown and pharmacological inhibition on 5-FU resistance in CRC was detected in cell models in vivo and in vitro.Results: Here we describe the condition of abnormal glucose metabolism in 5-FU-resistant CRC in detail, and we demonstrate that the enhanced glycolysis and pentose phosphate pathway in CRC are associated with increased HIF-1α expression. We also show that HIF-1α-induced glucose metabolic reprogramming imparts 5-FU resistance in CRC. HIF-1α showed enhanced expression in 5-FU-resistant CRC cells and clinical specimens, and increased HIF-1α levels were associated with failure of fluorouracil analog-based chemotherapy in CRC patients and poor survival. Upregulation of HIF-1α in 5-FU-resistant CRC occurs through non-oxygen-dependent mechanisms of reactive oxygen species-mediated activation of PI3K/Akt signaling, and aberrant activation of β-catenin in the nucleus. Both HIF-1α gene knock-down and pharmacological inhibition restored the sensitivity of CRC to 5-FU, indicating the potential efficacy of strategies targeting HIF-1α as an upstream glycolytic pathway regulator. Conclusions: Our results indicate HIF-1α is a potential biomarker for 5-FU-resistant CRC, and targeting HIF-1a in combination with 5-FU may represent an effective therapeutic strategy in 5-FU-resistant CRC.
Simple Fabrication and Carrier Type Modification of Semi-Conductive C/Al2O3 Composites by Powder Mixtures and Pulsed Electric Current Sintering
Authors: Nguyen Huu Hien , Xin Yunzi , Shirai Takashi 
Published: October 2021

Abstract
Semi-conductive C/Al2O3 ceramic composites were successfully fabricated by a pretty simple approach, combining a powder mixture process and pulsed electric current sintering. In order to obtain homogeneous distribution of carbon contents, popular polymers were used as carbon fillers to mix with Al2O3 powder by a wet ball milling step. The sintering was conducted by pulsed electric current sintering without any special requirement of the reductive environment like in conventional sintering. Density of bulk bodies, states of carbon contents after sintering and the electrical properties were analyzed in this study. Although the density of sintered bodies and their electrical properties were not superior, those characteristics of C/Al2O3 ceramic composites in this study were still comparatively high among semi-conductive ceramics. Especially, the carrier type of semi-conductive C/Al2O3 composites could be modified easily by adding polycarboxylic acid (PCA) in the powder mixture as a dispersant.
Class III malocclusion: a challenging treatment using miniscrews for extra anchorage
Authors: Matsumoto Mirian Aiko Nakane , Monteiro Patrícia Maria , Mendes Wendes Dias , Ferreira José Tarcísio Lima , Romano Fábio Lourenço , Stuani Maria Bernadete Sasso 
Published: October 2021

Abstract

Aims
This article presents the nonsurgical orthodontic treatment of a skeletal Class III malocclusion of an adult patient.

Methods
Because the patient refused an orthognathic surgical procedure, the mandibular first premolars were extracted and orthodontic camouflage using miniscrew anchorage was used to correct dental asymmetries and the occlusal relationship.

Results
The treatment strategy was successful and provided an acceptable aesthetic functional occlusion.

Conclusion
When appropriately indicated, the orthodontic camouflage of a class III malocclusion can avoid orthognathic surgery and, through the use of mini-implants as skeletal anchorage, enhance the results.
Possible Effect of Astaxanthin on Obesity-related Increased COVID-19
Infection Morbidity and Mortality
Authors: Örs Elif Didem , Alkan Şenay Burçin , Öksüz Abdullah 
Published: October 2021

Abstract:
Obesity is defined by the World Health Organisation (WHO) as a body mass index
equal to 30 kg/m2 or greater. It is an important and escalating global public health problem.
Obesity is known to cause low-grade chronic inflammation, increasing the burden of noncommunicable
and possibly communicable diseases. There is considerable evidence that obesity is
associated with an increased risk of contracting coronavirus disease 2019 (COVID-19) infection
as well as significantly higher COVID-19 morbidity and mortality. It appears plausible
that controlling the chronic systemic low-grade inflammation associated with obesity may have
a positive impact on the symptoms and the prognosis of COVID-19 disease in obese patients.
Astaxanthin (ASTX) is a naturally occurring carotenoid with anti-inflammatory, antioxidant,
and immunomodulatory activities. As a nutraceutical agent, it is used as a preventative and a
co-treatment in a number of systemic neurological, cardiovascular, and metabolic diseases.
This review article will discuss the pathogenesis of COVID-19 infection and the effect of
ASTX on obesity and obesity-related inflammation. The potential positive impact of ASTX anti-
inflammatory properties in obese COVID-19 patients will be discussed.
The Concurrent Therapeutic Potential of Adipose-derived Mesenchymal
Stem Cells on Gentamycin-induced Hepatorenal Toxicity in Rats
Authors: Mohamed Ayman Saber , Rawash Mohamed A. , El-Zayat Emad M. 
Published: October 2021

Background:
Adipose mesenchymal stem cells (AMSCs) are a type of stem cell employed
to repair damaged organs. This study aimed to see how effective AMSCs are at treating gentamycin-
induced hepatorenal damage in rats.

Methods:
18 male Wister rats were assigned into three groups; control, Gentamycin (GM), and
GM+AMSCs. GM induced hepatorenal toxicity through daily injection (100 mg/kg, i.p.) for eight
days. On day 9, AMSC (106 cells/ml/rat) was injected intravenously.

Results :
Creatinine, urea, uric acid, AST, ALP, ALT, TNF-, and MDA levels decreased, whereas
IL-10, GSH, and CAT levels increased, indicating the therapeutic potency of intravenous injection
AMSCs.

Conclusion:
The current study demonstrated the simultaneous therapeutic efficacy of adipose mesenchymal
stem cells on the liver and kidney in the treatment of Gentamycin-induced hepatotoxicity.
These data show that AMSCs could be a feasible therapy option for liver and kidney disease.
Natural Compounds as Heme Oxygenase-1 Inducers to Reduce the Deleterious Consequences Following SARS-CoV-2 Infection
Authors: Sorrenti Valeria , Consoli Valeria , Grosso S. Salvo , Vanella Luca 
Published: October 2021

:
The virus SARS-CoV-2 (Severe acute respiratory syndrome coronavirus 2) causes
COVID 19 (CoronaVIrus Disease 19), a global pandemic with multi-organ failure, resulting in
high morbidity and mortality. Some individuals are more vulnerable than others and have deleterious
consequences following covid- 19. It has been postulated that Heme oxygenase-1 (HO-1) reduction
and free heme may contribute to many of the inflammatory phenomena observed in
COVID-19 patients. Therefore, HO-1 inducers could prove to be potential therapeutic or preventive
agents for COVID 19. Many of the natural compounds present in fruits and vegetables, such as
polyphenols, were able to induce HO-1. The aim of this review is to focus on the main foods containing
bioactive compounds able to induce HO-1 for an informed choice of foods to use to counteract
damage from SARS-CoV-2 infection.
Review on Documented Medicinal Plants used for the Treatment of Cancer
Authors: Joshi Bhuwan C. , Juyal Vijay , Sah Archana N. , Verma Piyush , Mukhija Minky 
Published: October 2021

Background:Cancer is a frightful disease and it is the second leading cause of death worldwide. Naturally derived compounds are gaining interest of research workers as they have less toxic side effects as compared to currently used treatments, such as chemotherapy. Plants are the pool of chemical compounds, which provide a promising future for research on cancer.Objective:This review paper provides updated information gathered on medicinal plants and isolated phytoconstituents used as anticancer agents and summarises the plant extracts and their isolated chemical constituents exhibiting anticancer potential on clinical trials.Methods:An extensive bibliographic investigation was carried out by analysing worldwide established scientific databases like SCOPUS, PUBMED, SCIELO, ScienceDirect, Springerlink, Web of Science, Wiley, SciFinder and Google Scholar etc. In the next few decades, herbal medicine may become a new epoch of medical system.Results:Many researches are going on medicinal plants for the treatment of cancer but it is a time to increase further experimental studies on plant extracts and their chemical constituents to find out their mechanism of action at molecular level.Conclusion:The article may help many researchers to start off further experimentation that might lead to the drugs for the cancer treatment.
A Novel Intuitionistic Fuzzy Time Series Method Based on Bootstrapped Combined Pi-Sigma Artificial Neural Network
Authors: Bas Eren , Egrioglu Erol , Kölemen Emine 
Published: October 2021

Background:
Intuitionistic fuzzy time series forecasting methods have been started to solve the forecasting problems in the literature. Intuitionistic fuzzy time series methods use both membership and non-membership values as auxiliary variables in their models. Because intuitionistic fuzzy sets take into consideration the hesitation margin and so the intuitionistic fuzzy time series models use more information than fuzzy time series models. The background of this study is about intuitionistic fuzzy time series forecasting methods.

Objective:
The study aims to propose a novel intuitionistic fuzzy time series method. It is expected that the proposed method will produce better forecasts than some selected benchmarks.

Method:
The proposed method uses bootstrapped combined Pi-Sigma artificial neural network and intuitionistic fuzzy c-means. The combined Pi-Sigma artificial neural network is proposed to model the intuitionistic fuzzy relations.

Results and Conclusion:
The proposed method is applied to different sets of SP&500 stock exchange time series. The proposed method can provide more accurate forecasts than established benchmarks for the SP&500 stock exchange time series. The most important contribution of the proposed method is that it creates statistical inference: probabilistic forecasting, confidence intervals and the empirical distribution of the forecasts. Moreover, the proposed method is better than the selected benchmarks for the SP&500 data set.
Immersion Experiences in a Tablet-Based Markerless Augmented Reality Working Memory Game: Randomized Controlled Trial and User Experience Study
Authors: Zhang Bo , Robb Nigel 
Published: October 2021

Background
In recent years, augmented reality (AR), especially markerless augmented reality (MAR), has been used more prevalently to create training games in an attempt to improve humans' cognitive functions. This has been driven by studies claiming that MAR provides users with more immersive experiences that are situated in the real world. Currently, no studies have scientifically investigated the immersion experience of users in a MAR cognitive training game. Moreover, there is an observed lack of instruments on measuring immersion in MAR cognitive training games.

Objective
This study, using two existing immersion questionnaires, investigates students’ immersion experiences in a novel MAR n-back game.

Methods
The n-back task is a continuous performance task that taps working memory (WM) capacity. We compared two versions of n-back training. One was presented in a traditional 2D format, while the second version used MAR. There were 2 experiments conducted in this study that coordinated with 2 types of immersion questionnaires: the modified Immersive Experiences Questionnaire (IEQ) and the Augmented Reality Immersion (ARI) questionnaire. Two groups of students from two universities in China joined the study, with 60 participants for the first experiment (a randomized controlled experiment) and 51 participants for the second.

Results
Both groups of students experienced immersion in the MAR n-back game. However, the MAR n-back training group did not experience stronger immersion than the traditional (2D) n-back control group in the first experiment. The results of the second experiment showed that males felt deeply involved with the AR environment, which resulted in obtaining higher levels of immersion than females in the MAR n-back game.

Conclusions
Both groups of students experienced immersion in the MAR n-back game. Moreover, both the modified IEQ and ARI have the potential to be used as instruments to measure immersion in MAR game settings.

Trial Registration
UMIN Clinical Trials Registry UMIN000045314; https://upload.umin.ac.jp/cgi-open-bin/ctr_e/ctr_view.cgi?recptno=R000051725
Social Network Analysis (SNA) on #Kristengray Hashtag: Understanding Gentrification Side Effects Behind the Digital Nomad Phenomenon
Authors: Bahri Mohammad Thoriq , Widhyharto Derajad Sulistyo 
Published: October 2021

Indonesia is one of the destinations countries for digital nomads in South East Asia. Digital Nomad can be described by the person who combining work and leisure activities, without geographical border. The digital nomad lifestyle is becoming trends after an American tourist, Kristen Gray becoming viral on the Twitter Platform, she promoting Bali as a heaven for the digital nomad because of the low cost of living and cultural openness by selling eBook and open consultation services for the people who live in the America and European continents, but then she deported by the Indonesian Immigration Authorities, charged with the misuse of visas (she was used tourism visa for business purposes). Then, the research question arises, Are there potential gentrification effects of digital nomad lifestyle to the local people? And what the government should do to tackle this issue? This research is conducted by analyzing tweets using #kristengray and #digitalnomad hashtag, obtained from 12 January to 12 March 2021. The results are more than 49 percent of users, which dominated by the foreigner is defending Kristen Gray, and forcing the Indonesian Government to open Bali as digital nomad living places, but 51 percent of users, who dominated by Indonesian is refusing what the Kristen Gray doing, by arguing if Bali is purely just for tourism places. The results are the gentrification potentially happening between the digital nomad and local people, because of the difference in economic level, culture and value. Then, the government should consider making a policy to minimize the effects of gentrification resulted in digital nomad activities, suggested opening special visas with the restriction and tax policy for the digital nomad.
‘It Was Like a Space of Resistance’
Authors: Waxman Rebecca 
Published: October 2021

This article examines the function of music for female political prisoners during the Pinochet Dictatorship in Chile. The discussion draws on a thematic analysis of testimonies about music by female prisoners from the platform Cantos Cautivos (“Captive Songs”). The article discusses musical happenings, genres of music being performed (e.g., Nueva Canción), and the ways in which music-making was organised. In particular, the article highlights the importance of communal singing. This article also addresses cultural and collective memory and the role that they play in these recollections.
DIETARY ANALYSIS OF THREE SPECIES OF THE GENUS Anolis (SAURIA: DACTYLOIDAE) IN “LOS TUXTLAS,” VERACRUZ, MEXICO
Authors: Flores Villela Oscar Alberto 
Published: October 2021

Anoles have been studied by researchers to a greater extent than any other group of lizards. Their high diversity has led them to colonize a variety of niches, making them an ideal model group for evaluating ecological hypotheses such as dietary niche overlap. This work analyzes the stomach contents of 73 individuals from three species of the genus Anolis: A. barkeri (34), A. sericeus (17), and A. tropidonotus (22) occurring in the vicinity of Los Tuxtlas, Veracruz. Analyses performed included Shannon’s index in its log form to calculate dietary diversity, the Jaccard index to estimate the dissimilarity of the species’ diets, and Schoener’s index to measure dietary overlap. The results suggest that A. barkeri (10.08) hast the most generalist diet, followed by A. sericeus (8.75) and A. tropidonotus (5.78). Schoener’s index showed a considerable amount of diet overlap between A. barkeri and A. sericeus (0.76). We conclude that the three focal species show a generalist feeding behavior in times of abundant prey, such as the rainy season in which this study was conducted. This may lead to the elusion of intra-generic competition, explaining why we did not observe dietary niche displacement between these three species of Anolis.
Sintomatologia depressiva e suas repercussões na representação social da depressão: um estudo com adolescentes
Authors: Coutinho de Mello Alexandre , Carneiro Maciel Silvana , Vasconcelos Dias Camila Cristina , Cabral da Silva João Victor 
Published: October 2021

A depressão tem sido retratada como o mal do século, tornando-se um problema de saúde pública que tem afetado milhões de pessoas no mundo. Este estudo objetivou compreender como os adolescentes representam a depressão. Participaram 168 estudantes do ensino médio, com idades de 14 a 18 anos, de escolas públicas e privadas de João Pessoa-Paraíba, Brasil. Utilizou-se a Técnica de Associação Livre de Palavras (TALP), Questionário sociodemográfico e a Escala de Ansiedade e Depressão Hospitalar (HADS). Observou-se a representação social da depressão ancorada em vivências subjetivas enfatizando os aspectos individuais desse fenômeno como tristeza, solidão, angústia, sofrimento e suicídio. Os adolescentes apresentaram sintomatologia depressiva em 21,4 %, sendo estes percentuais acentuados quando comparados com outros estudos na área. Espera-se que este estudo possa promover ações de prevenção no contexto escolar e reflexões sobre meios de conscientização da depressão como um transtorno de causas múltiplas.