bluuebunny/crossref_metadata_2025_split huggingface dataset:
Parental Posttraumatic Stress Symptoms in the Context of Pediatric Post-Intensive Care Syndrome: Impact on the Family and Opportunities for Intervention
Authors: Riley Andrew R. , Williams Cydni N. , Moyer Danielle , Bradbury Kathryn , Leonard Skyler , Turner Elise , Holding Emily , Hall Trevor A. 
Published: August 2021

Objective: Pediatric intensive care unit (PICU) survivors and their families experience ongoing impacts on physical, cognitive, and psychosocial functioning, described as post-intensive care syndrome. The objective of this study was to determine whether the posttraumatic stress symptoms (PTSS) of parents predict the impact of critical illness on families following PICU admission beyond other factors (e.g., sex, race/ethnicity, age, insurance status, illness severity, family involvement or death). Method: We conducted a retrospective analysis of data from 88 children aged 1 month to 18 years who were hospitalized with critical illness and acquired brain injury in the PICU and their families. Patients and their families participated in a 1–3-month postdischarge follow-up assessment, during which data on demographics, medical diagnoses, parent self-report of PTSS, and family impact of critical illness (via the Pediatric Quality of Life Family Impact Module) were collected. We used a hierarchical linear regression to determine whether parent PTSS predicted family impact above and beyond demographic and injury/illness factors. Results: One third of parents reported elevated PTSS. Among those with complete available data ( n = 56), PTSS were the only significant predictor of family impact (β = −.52, t = −3.58, p = .001), with the overall model accounting for 41% of variance. Conclusion: In addition to the direct effects on parents of children who survive the PICU, PTSS may negatively impact families and interfere with rehabilitative progress. We provide a rationale and conceptual model for integrating interventions designed to address parent PTSS into post-PICU care. Implications for Impact Statement Parents’ posttraumatic stress symptoms predict the impact of child critical illness on the family beyond other factors. Accessible, effective interventions to address posttraumatic stress symptoms of the parents of pediatric intensive care survivors are needed.
Foundry-compatible high-resolution patterning of vertically phase-separated semiconducting films for ultraflexible organic electronics
Authors: Wang Binghao , Huang Wei , Lee Sunghoon , Huang Lizhen , Wang Zhi , Chen Yao , Chen Zhihua , Feng Liang-Wen , Wang Gang , Yokota Tomoyuki , Someya Takao , Marks Tobin J. , Facchetti Antonio 
Published: August 2021

AbstractSolution processability of polymer semiconductors becomes an unfavorable factor during the fabrication of pixelated films since the underlying layer is vulnerable to subsequent solvent exposure. A foundry-compatible patterning process must meet requirements including high-throughput and high-resolution patternability, broad generality, ambient processability, environmentally benign solvents, and, minimal device performance degradation. However, known methodologies can only meet very few of these requirements. Here, a facile photolithographic approach is demonstrated for foundry-compatible high-resolution patterning of known p- and n-type semiconducting polymers. This process involves crosslinking a vertically phase-separated blend of the semiconducting polymer and a UV photocurable additive, and enables ambient processable photopatterning at resolutions as high as 0.5 μm in only three steps with environmentally benign solvents. The patterned semiconducting films can be integrated into thin-film transistors having excellent transport characteristics, low off-currents, and high thermal (up to 175 °C) and chemical (24 h immersion in chloroform) stability. Moreover, these patterned organic structures can also be integrated on 1.5 μm-thick parylene substrates to yield highly flexible (1 mm radius) and mechanically robust (5,000 bending cycles) thin-film transistors.
Mirtron-mediated RNA knockdown/replacement therapy for the treatment of dominant retinitis pigmentosa
Authors: Orlans Harry O. , McClements Michelle E. , Barnard Alun R. , Martinez-Fernandez de la Camara Cristina , MacLaren Robert E. 
Published: August 2021

AbstractRhodopsin (RHO) gene mutations are a common cause of autosomal dominant retinitis pigmentosa (ADRP). The need to suppress toxic protein expression together with mutational heterogeneity pose challenges for treatment development. Mirtrons are atypical RNA interference effectors that are spliced from transcripts as short introns. Here, we develop a novel mirtron-based knockdown/replacement gene therapy for the mutation-independent treatment of RHO-related ADRP, and demonstrate efficacy in a relevant mammalian model. Splicing and potency of rhodopsin-targeting candidate mirtrons are initially determined, and a mirtron-resistant codon-modified version of the rhodopsin coding sequence is validated in vitro. These elements are then combined within a single adeno-associated virus (AAV) and delivered subretinally in a RhoP23H knock-in mouse model of ADRP. This results in significant mouse-to-human rhodopsin RNA replacement and is associated with a slowing of retinal degeneration. This provides proof of principle that synthetic mirtrons delivered by AAV are capable of reducing disease severity in vivo.
Temperature-dependent kinetic pathways of heterogeneous ice nucleation competing between classical and non-classical nucleation
Authors: Li Chu , Liu Zhuo , Goonetilleke Eshani C. , Huang Xuhui 
Published: August 2021

AbstractIce nucleation on the surface plays a vital role in diverse areas, ranging from physics and cryobiology to atmospheric science. Compared to ice nucleation in the bulk, the water-surface interactions present in heterogeneous ice nucleation complicate the nucleation process, making heterogeneous ice nucleation less comprehended, especially the relationship between the kinetics and the structures of the critical ice nucleus. Here we combine Markov State Models and transition path theory to elucidate the ensemble pathways of heterogeneous ice nucleation. Our Markov State Models reveal that the classical one-step and non-classical two-step nucleation pathways can surprisingly co-exist with comparable fluxes at T = 230 K. Interestingly, we find that the disordered mixing of rhombic and hexagonal ice leads to a favorable configurational entropy that stabilizes the critical nucleus, facilitating the non-classical pathway. In contrast, the favorable energetics promotes the formation of hexagonal ice, resulting in the classical pathway. Furthermore, we discover that, at elevated temperatures, the nucleation process prefers to proceed via the classical pathway, as opposed to the non-classical pathway, since the potential energy contributions override the configurational entropy compensation. This study provides insights into the mechanisms of heterogeneous ice nucleation and sheds light on the rational designs to control crystallization processes.
Surface evolution and corrosion behaviour of Cu-doped carbide-reinforced martensitic steels in a sulfuric acid solution
Authors: Yamanaka Kenta , Mori Manami , Yoshida Kazuo , Onuki Yusuke , Sato Shigeo , Chiba Akihiko 
Published: August 2021

AbstractCu-doped martensitic steels (Fe–(13, 16)Cr–3W–2Cu–1C) (mass%) with multiple carbide precipitates were prepared at different quenching temperatures, and their corrosion behaviours were examined by measuring the weight loss during immersion in a 0.5 M H2SO4 solution. Lower weight losses and corrosion rates were obtained for the alloy samples prepared at higher quenching temperatures. Surface Cu enrichment was observed for all specimens with a large fraction of dissolved Cr species. Moreover, quenching from higher temperatures not only reduced the amount of M23C6 carbide but also decreased the local electrochemical potential difference between the carbide phase and the martensitic matrix via enhanced surface Cu accumulation, thus increasing corrosion resistance by suppressing microgalvanic corrosion between the constituent phases. The corrosion behaviour of the studied steels was remarkably different from those of the Cu-doped stainless and low-alloy steels with passive oxide surface films, suggesting the strong effect of multiple carbide precipitates on their corrosion behaviour.
Bayesian Analysis Reporting Guidelines
Authors: Kruschke John K. 
Published: August 2021

AbstractPrevious surveys of the literature have shown that reports of statistical analyses often lack important information, causing lack of transparency and failure of reproducibility. Editors and authors agree that guidelines for reporting should be encouraged. This Review presents a set of Bayesian analysis reporting guidelines (BARG). The BARG encompass the features of previous guidelines, while including many additional details for contemporary Bayesian analyses, with explanations. An extensive example of applying the BARG is presented. The BARG should be useful to researchers, authors, reviewers, editors, educators and students. Utilization, endorsement and promotion of the BARG may improve the quality, transparency and reproducibility of Bayesian analyses.
Dynamics of a vibration-driven single disk
Authors: Guan Liyang , Tian Li , Hou Meiying , Han Yilong 
Published: August 2021

AbstractGranular particles exhibit rich collective behaviors on vibration beds, but the motion of an isolated particle is not well understood even for uniform particles with a simple shape such as disks or spheres. Here we measured the motion of a single disk confined to a quasi-two-dimensional horizontal box on a vertically vibrating stage. The translational displacements obey compressed exponential distributions whose exponent $$\beta$$
β
increases with the frequency, while the rotational displacements exhibit unimodal distributions at low frequencies and bimodal distributions at high frequencies. During short time intervals, the translational displacements are subdiffusive and negatively correlated, while the rotational displacements are superdiffusive and positively correlated. After prolonged periods, the rotational displacements become diffusive and their correlations decay to zero. Both the rotational and the translational displacements exhibit white noise at low frequencies, and blue noise for translational motions and Brownian noise for rotational motions at high frequencies. The translational kinetic energy obeys Boltzmann distribution while the rotational kinetic energy deviates from it. Most energy is distributed in translational motions at low frequencies and in rotational motions at high frequencies, which violates the equipartition theorem. Translational and rotational motions are not correlated. These experimental results show that the random diffusion of such driven particles is distinct from thermal motion in both the translational and rotational degrees of freedom, which poses new challenges to theory. The results cast new light on the motion of individual particles and the collective motion of driven granular particles.
Characterization of the virome associated with Haemagogus mosquitoes in Trinidad, West Indies
Authors: Ali Renee , Jayaraj Jayaraman , Mohammed Azad , Chinnaraja Chinnadurai , Carrington Christine V. F. , Severson David W. , Ramsubhag Adesh 
Published: August 2021

AbstractCurrently, there are increasing concerns about the possibility of a new epidemic due to emerging reports of Mayaro virus (MAYV) fever outbreaks in areas of South and Central America. Haemagogus mosquitoes, the primary sylvan vectors of MAYV are poorly characterized and a better understanding of the mosquito’s viral transmission dynamics and interactions with MAYV and other microorganisms would be important in devising effective control strategies. In this study, a metatranscriptomic based approach was utilized to determine the prevalence of RNA viruses in field-caught mosquitoes morphologically identified as Haemagogus janthinomys from twelve (12) forest locations in Trinidad, West Indies. Known insect specific viruses including the Phasi Charoen-like and Humaiata-Tubiacanga virus dominated the virome of the mosquitoes throughout sampling locations while other viruses such as the avian leukosis virus, MAYV and several unclassified viruses had a narrower distribution. Additionally, assembled contigs from the Ecclesville location suggests the presence of a unique uncharacterized picorna-like virus. Mapping of RNA sequencing reads to reference mitochondrial sequences of potential feeding host animals showed hits against avian and rodent sequences, which putatively adds to the growing body of evidence of a potentially wide feeding host-range for the Haemagogus mosquito vector.
The Bradyrhizobium diazoefficiens type III effector NopE modulates the regulation of plant hormones towards nodulation in Vigna radiata
Authors: Piromyou Pongdet , Nguyen Hien P. , Songwattana Pongpan , Boonchuen Pakpoom , Teamtisong Kamonluck , Tittabutr Panlada , Boonkerd Nantakorn , Alisha Tantasawat Piyada , Göttfert Michael , Okazaki Shin , Teaumroong Neung 
Published: August 2021

AbstractHost-specific legume-rhizobium symbiosis is strictly controlled by rhizobial type III effectors (T3Es) in some cases. Here, we demonstrated that the symbiosis of Vigna radiata (mung bean) with Bradyrhizobium diazoefficiens USDA110 is determined by NopE, and this symbiosis is highly dependent on host genotype. NopE specifically triggered incompatibility with V. radiata cv. KPS2, but it promoted nodulation in other varieties of V. radiata, including KPS1. Interestingly, NopE1 and its paralogue NopE2, which exhibits calcium-dependent autocleavage, yield similar results in modulating KPS1 nodulation. Furthermore, NopE is required for early infection and nodule organogenesis in compatible plants. Evolutionary analysis revealed that NopE is highly conserved among bradyrhizobia and plant-associated endophytic and pathogenic bacteria. Our findings suggest that V. radiata and B. diazoefficiens USDA110 may use NopE to optimize their symbiotic interactions by reducing phytohormone-mediated ETI-type (PmETI) responses via salicylic acid (SA) biosynthesis suppression.
Multi-muscle deep learning segmentation to automate the quantification of muscle fat infiltration in cervical spine conditions
Authors: Weber Kenneth A. , Abbott Rebecca , Bojilov Vivie , Smith Andrew C. , Wasielewski Marie , Hastie Trevor J. , Parrish Todd B. , Mackey Sean , Elliott James M. 
Published: August 2021

AbstractMuscle fat infiltration (MFI) has been widely reported across cervical spine disorders. The quantification of MFI requires time-consuming and rater-dependent manual segmentation techniques. A convolutional neural network (CNN) model was trained to segment seven cervical spine muscle groups (left and right muscles segmented separately, 14 muscles total) from Dixon MRI scans (n = 17, 17 scans < 2 weeks post motor vehicle collision (MVC), and 17 scans 12 months post MVC). The CNN MFI measures demonstrated high test reliability and accuracy in an independent testing dataset (n = 18, 9 scans < 2 weeks post MVC, and 9 scans 12 months post MVC). Using the CNN in 84 participants with scans < 2 weeks post MVC (61 females, 23 males, age = 34.2 ± 10.7 years) differences in MFI between the muscle groups and relationships between MFI and sex, age, and body mass index (BMI) were explored. Averaging across all muscles, females had significantly higher MFI than males (p = 0.026). The deep cervical muscles demonstrated significantly greater MFI than the more superficial muscles (p < 0.001), and only MFI within the deep cervical muscles was moderately correlated to age (r > 0.300, p ≤ 0.001). CNN’s allow for the accurate and rapid, quantitative assessment of the composition of the architecturally complex muscles traversing the cervical spine. Acknowledging the wider reports of MFI in cervical spine disorders and the time required to manually segment the individual muscles, this CNN may have diagnostic, prognostic, and predictive value in disorders of the cervical spine.
Demonstration of Shor’s factoring algorithm for N $$=$$ 21 on IBM quantum processors
Authors: Skosana Unathi , Tame Mark 
Published: August 2021

AbstractWe report a proof-of-concept demonstration of a quantum order-finding algorithm for factoring the integer 21. Our demonstration involves the use of a compiled version of the quantum phase estimation routine, and builds upon a previous demonstration. We go beyond this work by using a configuration of approximate Toffoli gates with residual phase shifts, which preserves the functional correctness and allows us to achieve a complete factoring of $$N=21$$

N
=
21

. We implemented the algorithm on IBM quantum processors using only five qubits and successfully verified the presence of entanglement between the control and work register qubits, which is a necessary condition for the algorithm’s speedup in general. The techniques we employ may be useful in carrying out Shor’s algorithm for larger integers, or other algorithms in systems with a limited number of noisy qubits.
Development and characterization of probiotic mucilage based edible films for the preservation of fruits and vegetables
Authors: Davachi Seyed Mohammad , Pottackal Neethu , Torabi Hooman , Abbaspourrad Alireza 
Published: August 2021

AbstractThere is growing interest among the public and scientific community toward the use of probiotics to potentially restore the composition of the gut microbiome. With the aim of preparing eco-friendly probiotic edible films, we explored the addition of probiotics to the seed mucilage films of quince, flax, and basil. These mucilages are natural and compatible blends of different polysaccharides that have demonstrated medical benefits. All three seed mucilage films exhibited high moisture retention regardless of the presence of probiotics, which is needed to help preserve the moisture/freshness of food. Films from flax and quince mucilage were found to be more thermally stable and mechanically robust with higher elastic moduli and elongation at break than basil mucilage films. These films effectively protected fruits against UV light, maintaining the probiotics viability and inactivation rate during storage. Coated fruits and vegetables retained their freshness longer than uncoated produce, while quince-based probiotic films showed the best mechanical, physical, morphological and bacterial viability. This is the first report of the development, characterization and production of 100% natural mucilage-based probiotic edible coatings with enhanced barrier properties for food preservation applications containing probiotics.
TPPU treatment of burned mice dampens inflammation and generation of bioactive DHET which impairs neutrophil function
Authors: Bergmann Christian B. , Hammock Bruce D. , Wan Debin , Gogolla Falk , Goetzman Holly , Caldwell Charles C. , Supp Dorothy M. 
Published: August 2021

AbstractOxylipins modulate the behavior of immune cells in inflammation. Soluble epoxide hydrolase (sEH) converts anti-inflammatory epoxyeicosatrienoic acid (EET) to dihydroxyeicosatrienoic acid (DHET). An sEH-inhibitor, TPPU, has been demonstrated to ameliorate lipopolysaccharide (LPS)- and sepsis-induced inflammation via EETs. The immunomodulatory role of DHET is not well characterized. We hypothesized that TPPU dampens inflammation and that sEH-derived DHET alters neutrophil functionality in burn induced inflammation. Outbred mice were treated with vehicle, TPPU or 14,15-DHET and immediately subjected to either sham or dorsal scald 28% total body surface area burn injury. After 6 and 24 h, interleukin 6 (IL-6) serum levels and neutrophil activation were analyzed. For in vitro analyses, bone marrow derived neutrophil functionality and mRNA expression were examined. In vivo, 14,15-DHET and IL-6 serum concentrations were decreased after burn injury with TPPU administration. In vitro, 14,15-DHET impaired neutrophil chemotaxis, acidification, CXCR1/CXCR2 expression and reactive oxygen species (ROS) production, the latter independent from p38MAPK and PI3K signaling. We conclude that TPPU administration decreases DHET post-burn. Furthermore, DHET downregulates key neutrophil immune functions and mRNA expression. Altogether, these data reveal that TPPU not only increases anti-inflammatory and inflammation resolving EET levels, but also prevents potential impairment of neutrophils by DHET in trauma.
Ectopic expression of a novel cold-resistance protein 1 from Brassica oleracea promotes tolerance to chilling stress in transgenic tomato
Authors: Wani Umer Majeed , Majeed Sheikh Tahir , Raja Vaseem , Wani Zubair Ahmad , Jan Nelofer , Andrabi Khursid Iqbal , John Riffat 
Published: August 2021

AbstractCold stress is considered as one of the major environmental factors that adversely affects the plant growth and distribution. Therefore, there arises an immediate need to cultivate effective strategies aimed at developing stress-tolerant crops that would boost the production and minimise the risks associated with cold stress. In this study, a novel cold-responsive protein1 (BoCRP1) isolated fromBrassica oleraceawas ectopically expressed in a cold susceptible tomato genotype Shalimar 1 and its function was investigated in response to chilling stress.BoCRP1was constitutively expressed in all the tissues ofB. oleraceaincluding leaf, root and stem. However, its expression was found to be significantly increased in response to cold stress. Moreover, transgenic tomato plants expressingBoCRP1exhibited increased tolerance to chilling stress (4 °C) with an overall improved rate of seed germination, increased root length, reduced membrane damage and increased accumulation of osmoprotectants. Furthermore, we observed increased transcript levels of stress responsive genes and enhanced accumulation of reactive oxygen species scavenging enzymes in transgenic plants on exposure to chilling stress. Taken together, these results strongly suggest thatBoCRP1is a promising candidate gene to improve the cold stress tolerance in tomato.
Gait change in tongue movement
Authors: Derrick Donald , Gick Bryan 
Published: August 2021

AbstractDuring locomotion, humans switch gaits from walking to running, and horses from walking to trotting to cantering to galloping, as they increase their movement rate. It is unknown whether gait change leading to a wider movement rate range is limited to locomotive-type behaviours, or instead is a general property of any rate-varying motor system. The tongue during speech provides a motor system that can address this gap. In controlled speech experiments, using phrases containing complex tongue-movement sequences, we demonstrate distinct gaits in tongue movement at different speech rates. As speakers widen their tongue-front displacement range, they gain access to wider speech-rate ranges. At the widest displacement ranges, speakers also produce categorically different patterns for their slowest and fastest speech. Speakers with the narrowest tongue-front displacement ranges show one stable speech-gait pattern, and speakers with widest ranges show two. Critical fluctuation analysis of tongue motion over the time-course of speech revealed these speakers used greater effort at the beginning of phrases—such end-state-comfort effects indicate speech planning. Based on these findings, we expect that categorical motion solutions may emerge in any motor system, providing that system with access to wider movement-rate ranges.