bluuebunny/crossref_metadata_2025_split huggingface dataset:
Compartmentalized replication organelle of flavivirus at the ER and the factors involved
Authors: Ci Yali , Shi Lei 
Published: April 2021

AbstractFlaviviruses are positive-sense single-stranded RNA viruses that pose a considerable threat to human health. Flaviviruses replicate in compartmentalized replication organelles derived from the host endoplasmic reticulum (ER). The characteristic architecture of flavivirus replication organelles includes invaginated vesicle packets and convoluted membrane structures. Multiple factors, including both viral proteins and host factors, contribute to the biogenesis of the flavivirus replication organelle. Several viral nonstructural (NS) proteins with membrane activity induce ER rearrangement to build replication compartments, and other NS proteins constitute the replication complexes (RC) in the compartments. Host protein and lipid factors facilitate the formation of replication organelles. The lipid membrane, proteins and viral RNA together form the functional compartmentalized replication organelle, in which the flaviviruses efficiently synthesize viral RNA. Here, we reviewed recent advances in understanding the structure and biogenesis of flavivirus replication organelles, and we further discuss the function of virus NS proteins and related host factors as well as their roles in building the replication organelle.
Random attractors via pathwise mild solutions for stochastic parabolic evolution equations
Authors: Kuehn Christian , Neamţu Alexandra , Sonner Stefanie 
Published: April 2021

AbstractWe investigate the longtime behavior of stochastic partial differential equations (SPDEs) with differential operators that depend on time and the underlying probability space. In particular, we consider stochastic parabolic evolution problems in Banach spaces with additive noise and prove the existence of random exponential attractors. These are compact random sets of finite fractal dimension that contain the global random attractor and are attracting at an exponential rate. In order to apply the framework of random dynamical systems, we use the concept of pathwise mild solutions.
An axiomatic approach to existence and liveness for differential equations
Authors: Tan Yong Kiam , Platzer André 
Published: April 2021

AbstractThis article presents an axiomatic approach for deductive verification of existence and liveness for ordinary differential equations (ODEs) with differential dynamic logic (dL). The approach yields proofs that the solution of a given ODE exists long enough to reach a given target region without leaving a given evolution domain. Numerous subtleties complicate the generalization of discrete liveness verification techniques, such as loop variants, to the continuous setting. For example, ODE solutions may blow up in finite time or their progress towards the goal may converge to zero. These subtleties are handled in dL by successively refining ODE liveness properties using ODE invariance properties which have a complete axiomatization. This approach is widely applicable: several liveness arguments from the literature are surveyed and derived as special instances of axiomatic refinement in dL. These derivations also correct several soundness errors in the surveyed literature, which further highlights the subtlety of ODE liveness reasoning and the utility of an axiomatic approach. An important special case of this approach deduces (global) existence properties of ODEs, which are a fundamental part of every ODE liveness argument. Thus, all generalizations of existence properties and their proofs immediately lead to corresponding generalizations of ODE liveness arguments. Overall, the resulting library of common refinement steps enables both the sound development and justification of new ODE existence and of liveness proof rules from dL axioms. These insights are put into practice through an implementation of ODE liveness proofs in the KeYmaera X theorem prover for hybrid systems.
The African tragedy: the effect of democracy on economic growth
Authors: Khodaverdian Saeed 
Published: April 2021

AbstractWe estimate the effect of democracy on economic growth for the countries in Sub-Saharan Africa in comparison with other countries. We find that in contrast to other countries, democracy in Africa benefits neither GDP per capita nor total GDP. We explain the former by changes in the size of the population and the latter by changes in the age structure of the population. Both demographic changes relate to the finding that unlike in other countries, democracy does not reduce child mortality in Africa. The evidence suggests that without improvements in health, democracy puts Africa on a path toward a Malthusian trap.
Legitimate equilibrium
Authors: Flesch János , Vermeulen Dries , Zseleva Anna 
Published: April 2021

AbstractWe present a general existence result for a type of equilibrium in normal-form games, which extends the concept of Nash equilibrium. We consider nonzero-sum normal-form games with an arbitrary number of players and arbitrary action spaces. We impose merely one condition: the payoff function of each player is bounded. We allow players to use finitely additive probability measures as mixed strategies. Since we do not assume any measurability conditions, for a given strategy profile the expected payoff is generally not uniquely defined, and integration theory only provides an upper bound, the upper integral, and a lower bound, the lower integral. A strategy profile is called a legitimate equilibrium if each player evaluates this profile by the upper integral, and each player evaluates all his possible deviations by the lower integral. We show that a legitimate equilibrium always exists. Our equilibrium concept and existence result are motivated by Vasquez (2017), who defines a conceptually related equilibrium notion, and shows its existence under the conditions of finitely many players, separable metric action spaces and bounded Borel measurable payoff functions. Our proof borrows several ideas from (Vasquez (2017)), but is more direct as it does not make use of countably additive representations of finitely additive measures by (Yosida and Hewitt (1952)).
Nalmefene attenuates neural alcohol cue-reactivity in the ventral striatum and subjective alcohol craving in patients with alcohol use disorder
Authors: Karl Damian , Bumb J. Malte , Bach Patrick , Dinter Christina , Koopmann Anne , Hermann Derik , Mann Karl , Kiefer Falk , Vollstädt-Klein Sabine 
Published: April 2021

Abstract
Rationale
Alcohol use disorder is a common and devastating mental illness for which satisfactory treatments are still lacking. Nalmefene, as an opioid receptor modulator, could pharmacologically support the reduction of drinking by reducing the (anticipated) rewarding effects of alcohol and expanding the range of treatment options. It has been hypothesized that nalmefene acts via an indirect modulation of the mesolimbic reward system. So far, only a few imaging findings on the neuronal response to nalmefene are available.

Objectives
We tested the effect of a single dose of 18 mg nalmefene on neuronal cue-reactivity in the ventral and dorsal striatum and subjective craving.

Methods
Eighteen non-treatment-seeking participants with alcohol use disorder (67% male, M = 50.3 ± 13.9 years) with a current high-risk drinking level (M = 76.9 ± 52 g of pure alcohol per day) were investigated using a cue-reactivity task during functional magnetic resonance imaging (fMRI) in a double-blind, placebo-controlled, cross-over study/design. In addition, self-reported craving was assessed before and after exposure to alcohol cues.

Results
An a priori defined region of interest (ROI) analysis of fMRI data from 15 participants revealed that nalmefene reduced alcohol cue-reactivity in the ventral, but not the dorsal striatum. Additionally, the subjective craving was significantly reduced after the cue-reactivity task under nalmefene compared to placebo.

Conclusion
In the present study, reduced craving and cue-reactivity to alcohol stimuli in the ventral striatum by nalmefene indicates a potential anti-craving effect of this drug via attenuation of neural alcohol cue-reactivity.
In vivo evidence for the unique kinetics of evoked dopamine release in the patch and matrix compartments of the striatum
Authors: Jaquins-Gerstl Andrea , Nesbitt Kathryn M. , Michael Adrian C. 
Published: April 2021

AbstractThe neurochemical transmitter dopamine (DA) is implicated in a number of diseases states, including Parkinson’s disease, schizophrenia, and drug abuse. DA terminal fields in the dorsal striatum and core region of the nucleus accumbens in the rat brain are organized as heterogeneous domains exhibiting fast and slow kinetic of DA release. The rates of dopamine release are significantly and substantially faster in the fast domains relative to the slow domains. The striatum is composed of a mosaic of spatial compartments known as the striosomes (patches) and the matrix. Extensive literature exists on the spatial organization of the patch and matrix compartments and their functions. However, little is known about these compartments as they relate to fast and slow kinetic DA domains observed by fast scan cyclic voltammetry (FSCV). Thus, we combined high spatial resolution of FSCV with detailed immunohistochemical analysis of these architectural compartments (patch and matrix) using fluorescence microscopy. Our findings demonstrated a direct correlation between patch compartments with fast domain DA kinetics and matrix compartments to slow domain DA kinetics. We also investigated the kinetic domains in two very distinct sub-regions in the striatum, the lateral dorsal striatum (LDS) and the medial dorsal striatum (MDS). The lateral dorsal striatum as opposed to the medial dorsal striatum is mainly governed by fast kinetic DA domains. These finding are highly relevant as they may hold key promise in unraveling the fast and slow kinetic DA domains and their physiological significance.Graphical abstract
Influence of different leavening agents on technological and nutritional characteristics of whole grain breads obtained from ancient and modern flour varieties
Authors: Venturi Manuel , Galli Viola , Pini Niccolò , Guerrini Simona , Sodi Costanza , Granchi Lisa 
Published: April 2021

AbstractThe aim of the work was to assess the influence of the leavening agent on several technological, chemical, and nutritional characteristics of breads prepared with whole soft wheat (Triticum aestivum L.) flours. As leavening agents in bread-making, baker’s yeast, biga, and sourdough were utilized. Two ancient varieties, Andriolo and Verna, and a modern grain variety, Bologna, were used. Analysis of the obtained breads included the measurement of the phenols content, the antioxidant activity, the in vitro protein and total digestibility, the texture profile, the crumb grain characteristics and the microbial shelf-life test. The results of the principle component analysis of bread features indicated a clustering depending especially on the leavening agent rather than on the employed flour, particularly when sourdough was used. Protein digestibility, crumb grain characteristics, and shelf-life led to the main differences among the samples. Ancient wheat flour displayed similar features when the same leavening agent was applied. Particularly, the use of sourdough levelled the differences due to flour, leading to breads with similar technological and nutritional characteristics. The findings highlighted a marked effect of the leavening agent on bread final characteristics.
A multisite analysis of the concordance between visual image interpretation and quantitative analysis of [18F]flutemetamol amyloid PET images
Authors: Bucci Marco , Savitcheva Irina , Farrar Gill , Salvadó Gemma , Collij Lyduine , Doré Vincent , Gispert Juan Domingo , Gunn Roger , Hanseeuw Bernard , Hansson Oskar , Shekari Mahnaz , Lhommel Renaud , Molinuevo José Luis , Rowe Christopher , Sur Cyrille , Whittington Alex , Buckley Christopher , Nordberg Agneta 
Published: April 2021

Abstract
Background
[18F]flutemetamol PET scanning provides information on brain amyloid load and has been approved for routine clinical use based upon visual interpretation as either negative (equating to none or sparse amyloid plaques) or amyloid positive (equating to moderate or frequent plaques). Quantitation is however fundamental to the practice of nuclear medicine and hence can be used to supplement amyloid reading methodology especially in unclear cases.

Methods
A total of 2770 [18F]flutemetamol images were collected from 3 clinical studies and 6 research cohorts with available visual reading of [18F]flutemetamol and quantitative analysis of images. These were assessed further to examine both the discordance and concordance between visual and quantitative imaging primarily using thresholds robustly established using pathology as the standard of truth. Scans covered a wide range of cases (i.e. from cognitively unimpaired subjects to patients attending the memory clinics). Methods of quantifying amyloid ranged from using CE/510K cleared marked software (e.g. CortexID, Brass), to other research-based methods (e.g. PMOD, CapAIBL). Additionally, the clinical follow-up of two types of discordance between visual and quantitation (V+Q- and V-Q+) was examined with competing risk regression analysis to assess possible differences in prediction for progression to Alzheimer’s disease (AD) and other diagnoses (OD).

Results
Weighted mean concordance between visual and quantitation using the autopsy-derived threshold was 94% using pons as the reference region. Concordance from a sensitivity analysis which assessed the maximum agreement for each cohort using a range of cut-off values was also estimated at approximately 96% (weighted mean). Agreement was generally higher in clinical cases compared to research cases. V-Q+ discordant cases were 11% more likely to progress to AD than V+Q- for the SUVr with pons as reference region.

Conclusions
Quantitation of amyloid PET shows a high agreement vs binary visual reading and also allows for a continuous measure that, in conjunction with possible discordant analysis, could be used in the future to identify possible earlier pathological deposition as well as monitor disease progression and treatment effectiveness.
Experimental investigation of equivalence ratio fluctuations in a lean premixed kerosene combustor
Authors: Vogel Manuel , Bachfischer Michael , Kaufmann Jan , Sattelmayer Thomas 
Published: April 2021

Abstract
In this study a measurement technique for determination of equivalence ratio fluctuations from flame chemiluminescence in a kerosene-fuelled lean premixed combustor under atmospheric conditions is presented. Firstly, fundamental investigations into the relationship between the ratio of different chemiluminescence signals and the equivalence ratio are carried out using an imaging spectrometer. The chemiluminescence intensity is recorded for a wide range of equivalence ratios and fuel mass flows during steady state operation. The spectra show that the CH*/OH* ratio depends linearly on the equivalence ratio and is independent of the mass flow in the investigated range. Moreover, the background radiation has no influence on the monotonous trend of the CH*/OH* ratio for kerosene combustion. This interesting finding opens up new possibilities for passive optical measurement of the equivalence ratio in kerosene flames. Bandpass-filtered phase-correlated images of OH* and CH* chemiluminescence of an acoustically excited flame are taken simultaneously on one camera chip using an image doubler. After distortion correction, the image pair is used to calculate the global equivalence ratio from the CH*/OH* ratio. Based on the calibration chart derived in stationary operation, phase-resolved equivalence ratio perturbations are determined during acoustic excitation. The presented technique allows a quantitative measurement of equivalence ratio fluctuations in spray combustion and can therefore provide a better understanding of the fundamental mechanisms of thermoacoustic instabilities triggered by equivalence ratio fluctuations.

Graphic abstract
Higher mortality of the less suitable brown trout host compared to the principal Atlantic salmon host when infested with freshwater pearl mussel (Margaritifera margaritifera) glochidia
Authors: Marwaha Janhavi , Jakobsen Per Johan , Karlsson Sten , Larsen Bjørn Mejdell , Wacker Sebastian 
Published: April 2021

AbstractThe freshwater pearl mussel (Margaritifera margaritifera) is a highly host-specific parasite, with an obligate parasitic stage on salmonid fish. Atlantic salmon (Salmo salar) and brown trout (Salmo trutta f. truttaandSalmo trutta f. fario) are the only hosts in their European distribution. SomeM. margaritiferapopulations exclusively infest either Atlantic salmon or brown trout, while others infest both hosts with one salmonid species typically being the principal host and the other a less suitable host. Glochidial abundance, prevalence and growth are often used as parameters to measure host suitability, with the most suitable host species displaying the highest parameters. However, it is not known if the degree of host specialisation will negatively influence host fitness (virulence) among different host species. In this study we examined the hypothesis that glochidial infestation would result in differential virulence in two salmonid host species and that lower virulence would be observed on the most suitable host. Atlantic salmon and brown trout were infested with glochidia from twoM. margaritiferapopulations that use Atlantic salmon as their principal host, and the difference in host mortality among infested and control (sham infested) fish was examined. Higher mortality was observed in infested brown trout (the less suitable host) groups, compared to the other test groups. Genetic assignment was used to identify offspring from individual mother mussels. We found that glochidia from individual mothers can infest both the salmonid hosts; however, some mothers displayed a bias towards either salmon or trout. We believe that the differences in host-dependent virulence and the host bias displayed by individual mothers were a result of genotype × genotype interactions between the glochidia and their hosts, indicating that there is an underlying genetic component for this parasite-host interaction.
Experience with 10 years of a robotic surgery program at an Academic Medical Center
Authors: Stringfield Sarah B. , Parry Lisa A. , Eisenstein Samuel G. , Horgan Santiago N. , Kane Christopher J. , Ramamoorthy Sonia L. 
Published: April 2021

Abstract
Background
Few studies have examined robotic surgery from a programmatic standpoint, yet this is how hospitals evaluate return on investment clinically and fiscally. This study examines the 10-year experience of a robotic program at a single academic institution.

Study design
All robotic operations performed at our institution from August 2005 to December 2016 were reviewed. Data were collected from the robotic system and hospital databases.

Results
A total of 3485 robotic operations were performed. Yearly case volume nearly quadrupled. There have been 37 robotic-trained surgeons in 5 specialties performing 53 different operations. Rate of conversion to open was 4.2%. American Society of Anesthesiologists (ASA) class increased over time, with ASA class 3 increasing from 20% of patients to 45% of patients.
Average case time in 2005 was 453 min, but decreased by 46% to 246 min by 2007, then remained relatively stable (range 226–247). Operating efficiency improved, with room time and case time decreasing by 9% in the past 4 years.
Average cost for robotic supplies was $1519 per case. Additional costs per case related to equipment and contracts totaled an average of $11,822.
Average length of stay (LOS) for robotic cases was 3.3 days, compared to 3.0 days for laparoscopic and 7.0 for open. Cost per day for admission after robotic surgery was 1.7 times greater than the cost of open or laparoscopic surgery. Total admission costs of robotic operations were 1.5 times those of laparoscopic surgery, but less than open operations. Readmissions following robotic cases were lower than open (15% v 26%, p < 0.0001).

Conclusions
Over 10 years, the use of robotic technology has grown significantly at our institution, with good fiscal and clinical outcomes. Operating room costs are high; however, efficiency has improved, LOS is shorter, admission costs are lower than open operations, and readmission rates are lower.
Reduction in postoperative complications by robotic surgery: a case–control study of robotic versus conventional laparoscopic surgery for gastric cancer
Authors: Kinoshita Takahiro , Sato Reo , Akimoto Eigo , Tanaka Yuya , Okayama Takafumi , Habu Takumi 
Published: April 2021

Abstract
Background
Robotic gastrectomy (RG) is being increasingly performed globally; it is considered an evolved type of conventional laparoscopic surgery with excellent dexterity and precision, but higher costs and longer operation time. Thus, there is a need to identify the benefits from RG and its specific candidates.

Methods
This retrospective study analyzed data from a prospectively collected clinical database at our center. Data of patients with primary gastric cancer undergoing either robotic or laparoscopic radical gastrectomy from June 2014 to June 2020 were reviewed. Surgical outcomes were compared between the two groups, and multivariable analyses were performed to elucidate the relevant factors for postoperative complications in several subgroups.

Results
A total of 1172 patients were divided into those who underwent RG (n = 152) and those who underwent laparoscopic gastrectomy (LG) (n = 1020). Baseline characteristics were similar in the two groups, except the RG group included more patients undergoing total/proximal gastrectomy (TG/PG) and patients at clinical stage III. Compared with the LG group, the RG group had lower incidences of postoperative complications ≥ Clavien-Dindo grade III (2/152 (1.3%) versus 72/1020 (7.1%); P = 0.004), and intraabdominal complications ≥ grade II (6/152 (3.9%) versus 119/1020 (11.7%); P = 0.004). Multivariable analysis revealed that RG was a significant relevant factor for reducing overall postoperative complications (≥ grade III) (odds ratio (OR) 0.16, P = 0.013), and intraabdominal complications (≥ grade II) (OR 0.29, P = 0.002). Subgroup analyses demonstrated that this tendency was enhanced in patients undergoing TG/PG (OR 0.29, P = 0.021) or at clinical stage II/III (OR 0.10, P = 0.027).

Conclusions
RG reduces the incidence of postoperative complications compared with conventional LG and this tendency may be enhanced in technically complicated procedures with demanding anastomosis or D2 lymphadenectomy. Patients requiring such procedures would most benefit from RG.
Advancing synthesis of decision tree-based multiple classifier systems: an approximate computing case study
Authors: Barbareschi Mario , Barone Salvatore , Mazzocca Nicola 
Published: April 2021

AbstractSo far, multiple classifier systems have been increasingly designed to take advantage of hardware features, such as high parallelism and computational power. Indeed, compared to software implementations, hardware accelerators guarantee higher throughput and lower latency. Although the combination of multiple classifiers leads to high classification accuracy, the required area overhead makes the design of a hardware accelerator unfeasible, hindering the adoption of commercial configurable devices. For this reason, in this paper, we exploit approximate computing design paradigm to trade hardware area overhead off for classification accuracy. In particular, starting from trained DT models and employing precision-scaling technique, we explore approximate decision tree variants by means of multiple objective optimization problem, demonstrating a significant performance improvement targeting field-programmable gate array devices.