bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mucoepidermoid carcinoma of the salivary glands revisited with special reference to histologic grading and CRTC1/3-MAML2 genotyping
Authors: Fehr André , Werenicz Sarah , Trocchi Pietro , Falk Markus , Friedrich Reinhard E. , Stammler Angelika , Stang Andreas , Oesterling Florian , Khil Laura , Stenman Göran , Böcker Werner , Tiemann Katharina , Löning Thomas 
Published: July 2021

AbstractMucoepidermoid carcinoma (MEC) is the most common carcinoma of the salivary glands. Here, we have used two large patient cohorts with MECs comprising 551 tumors to study clinical, histological, and molecular predictors of survival. One cohort (n = 167), with known CRCT1/3-MAML2 fusion status, was derived from the Hamburg Reference Centre (HRC; graded with the AFIP and Brandwein systems) and the other (n = 384) was derived from the population-based Cancer Registry of North Rhine-Westphalia (LKR-NRW; graded with the AFIP system). The reliability of both the AFIP and Brandwein grading systems was excellent (n = 155). The weighted kappa for inter-rater agreement was 0.81 (95% CI 0.65–0.97) and 0.83 (95% CI 0.71–0.96) for the AFIP and Brandwein systems, respectively. The 5-year relative survival was 79.7% (95% CI 73.2–86.2%). Although the Brandwein system resulted in a higher rate of G3-MECs, survival in G3-tumors (AFIP or Brandwein grading) was markedly worse than in G1/G2-tumors. Survival in > T2 tumors was markedly worse than in those with lower T-stage. Also, fusion-negative MECs had a worse 5-year progression-free survival. The frequency of fusion-positive MECs in the HRC cohort was 78.4%, of which the majority (86.7%) was G1/G2-tumors. In conclusion, the AFIP and Brandwein systems are useful in estimating prognosis and to guide therapy for G3-MECs. However, their significance regarding young age (≤ 30 years) and location-dependent heterogeneity of in particular G2-tumors is more questionable. We conclude that CRTC1/3-MAML2 testing is a useful adjunct to histologic scoring of MECs and for pinpointing tumors with poor prognosis with higher precision, thus avoiding overtreatment.
Complementary and alternative medicine (CAM) supplements in cancer outpatients: analyses of usage and of interaction risks with cancer treatment
Authors: Wolf Clemens P. J. G. , Rachow Tobias , Ernst Thomas , Hochhaus Andreas , Zomorodbakhsch Bijan , Foller Susan , Rengsberger Matthias , Hartmann Michael , Huebner Jutta 
Published: July 2021

Abstract
Purpose
The aim of our study was to analyze the use of complementary and alternative medicine (CAM) supplements, identify possible predictors, and analyze and compile potential interactions of CAM supplements with conventional cancer therapy.

Methods
We included outpatient cancer patients treated at a German university hospital in March or April 2020. Information was obtained from questionnaires and patient records. CAM–drug interactions were identified based on literature research for each active ingredient of the supplements consumed by the patients.

Results
37.4% of a total of 115 patients consumed CAM supplements. Potential interactions with conventional cancer treatment were identified in 51.2% of these patients. All types of CAM supplements were revealed to be a potential source for interactions: vitamins, minerals, food and plant extracts, and other processed CAM substances. Younger age (< 62 years) (p = 0.020, φc = 0.229) and duration of individual cancer history of more than 1 year (p = 0.006, φc = 0.264) were associated with increased likelihood of CAM supplement use. A wide range of different CAM supplement interactions were reviewed: effects of antioxidants, cytochrome (CYP) interactions, and specific agonistic or antagonistic effects with cancer treatment.

Conclusion
The interaction risks of conventional cancer therapy with over-the-counter CAM supplements seem to be underestimated. Supplements without medical indication, as well as overdoses, should be avoided, especially in cancer patients. To increase patient safety, physicians should address the risks of interactions in physician–patient communication, document the use of CAM supplements in patient records, and check for interactions.
Linking Numbers in Three-Manifolds
Authors: Cahn Patricia , Kjuchukova Alexandra 
Published: July 2021

AbstractLet M be a connected, closed, oriented three-manifold and K, L two rationally null-homologous oriented simple closed curves in M. We give an explicit algorithm for computing the linking number between K and L in terms of a presentation of M as an irregular dihedral three-fold cover of $$S^3$$

S
3

branched along a knot $$\alpha \subset S^3$$

α
⊂

S
3

. Since every closed, oriented three-manifold admits such a presentation, our results apply to all (well-defined) linking numbers in all three-manifolds. Furthermore, ribbon obstructions for a knot $$\alpha $$
α
can be derived from dihedral covers of $$\alpha $$
α
. The linking numbers we compute are necessary for evaluating one such obstruction. This work is a step toward testing potential counter-examples to the Slice-Ribbon Conjecture, among other applications.
Effects of Soil Abiotic and Biotic Factors on Tree Seedling Regeneration Following a Boreal Forest Wildfire
Authors: Ibáñez Theresa S. , Wardle David A. , Gundale Michael J. , Nilsson Marie-Charlotte 
Published: July 2021

AbstractWildfire disturbance is important for tree regeneration in boreal ecosystems. A considerable amount of literature has been published on how wildfires affect boreal forest regeneration. However, we lack understanding about how soil-mediated effects of fire disturbance on seedlings occur via soil abiotic properties versus soil biota. We collected soil from stands with three different severities of burning (high, low and unburned) and conducted two greenhouse experiments to explore how seedlings of tree species (Betula pendula, Pinus sylvestris and Picea abies) performed in live soils and in sterilized soil inoculated by live soil from each of the three burning severities. Seedlings grown in live soil grew best in unburned soil. When sterilized soils were reinoculated with live soil, seedlings of P. abies and P. sylvestris grew better in soil from low burn severity stands than soil from either high severity or unburned stands, demonstrating that fire disturbance may favor post-fire regeneration of conifers in part due to the presence of soil biota that persists when fire severity is low or recovers quickly post-fire. Betula pendula did not respond to soil biota and was instead driven by changes in abiotic soil properties following fire. Our study provides strong evidence that high fire severity creates soil conditions that are adverse for seedling regeneration, but that low burn severity promotes soil biota that stimulates growth and potential regeneration of conifers. It also shows that species-specific responses to abiotic and biotic soil characteristics are altered by variation in fire severity. This has important implications for tree regeneration because it points to the role of plant–soil–microbial feedbacks in promoting successful establishment, and potentially successional trajectories and species dominance in boreal forests in the future as fire regimes become increasingly severe through climate change.
A matter of frailty: the modified Subdural Hematoma in the Elderly (mSHE) score
Authors: Hernández-Durán Silvia , Behme Daniel , Rohde Veit , von der Brelie Christian 
Published: July 2021

AbstractThe Subdural Hematoma in the Elderly (SHE) score was developed as a model to predict 30-day mortality from acute, chronic, and mixed subdural hematoma in the elderly population after minor or no trauma. Emerging evidence suggests frailty to be predictive of mortality and morbidity in the elderly. In this study, we aim to externally validate the SHE for chronic subdural hematoma (CSDH) alone, and we hypothesize that the incorporation of frailty into the SHE may increase its predictive power. A retrospective cohort of elderly patients with CSDH after minor or no trauma being treated at our institution was evaluated with the SHE. Thirty-day mortality and outcome were documented. Patients were assessed with the Clinical Frailty Scale (CFS), which was incorporated into a modified SHE (mSHE). Both the SHE and the mSHE were then assessed in their predictive powers through receiver operating characteristic statistics. We included 168 patients. Most (n = 124, 74%) had a favorable outcome at 30 days. Mortality was low at n = 7, 4%. The SHE failed to predict mortality (AUC = .564, p = .565). Contrarily, the mSHE performed well in both mortality (AUC = .749, p = .026) and outcome (AUC = .862, p < .001). A threshold of mSHE = 3 is predictive of mortality with a sensitivity of 50% and a specificity of 75% and of poor outcome with a sensitivity of 88% and a specificity of 64%. Frailty should be routinely evaluated in elderly individuals, as it can predict outcome and mortality, providing the possibility for medical, surgical, nutritional, cognitive, and physical exercise interventions.
Diagnostic utility of a novel magnifying endoscopic classification system for superficial Barrett’s esophagus-related neoplasms: a nationwide multicenter study
Authors: Goda Kenichi , Takeuchi Manabu , Ishihara Ryu , Fujisaki Junko , Takahashi Akiko , Takaki Yasuhiro , Hirasawa Dai , Momma Kumiko , Amano Yuji , Yagi Kazuyoshi , Furuhashi Hiroto , Hashimoto Satoru , Kanesaka Takashi , Shimizu Tomoki , Ono Yoichiro , Yamagata Taku , Fujiwara Junko , Azumi Takane , Watanabe Gen , Ohkura Yasuo , Nishikawa Masako , Oyama Tsuneo 
Published: July 2021

Abstract
Background
Currently, no classification system using magnification endoscopy for the diagnosis of superficial Barrett’s esophagus (BE)-related neoplasia has been widely accepted. This nationwide multicenter study aimed to validate the diagnostic accuracy and reproducibility of the magnification endoscopy classification system, including the diagnostic flowchart developed by the Japan Esophageal Society—Barrett’s esophagus working group (JES-BE) for superficial Barrett’s esophagus-related neoplasms.

Methods
The JES-BE acquired high-definition magnification narrow-band imaging (HM-NBI) images of non-dysplastic and dysplastic BE from 10 domestic institutions. A total of 186 high-quality HM-NBI images were selected. Thirty images were used for the training phase and 156 for the validation (test) phase. We invited five non-experts and five expert reviewers. In the training phase, the reviewers discussed how to correctly predict the histology based on the JES-BE criteria. In the validation phase, they evaluated whether the criteria accurately predicted the histology results according to the diagnostic flowchart. The validation phase was performed immediately after the training phase and at 6 weeks thereafter.

Results
The sensitivity and specificity for all reviewers were 87% and 97%, respectively. Overall accuracy, positive predictive value, and negative predictive value were 91%, 98%, and 83%, respectively. The overall strength of inter-observer and intra-observer agreements for dysplastic histology prediction was κ = 0.77 and κ = 0.83, respectively. No significant difference in diagnostic accuracy and reproducibility between experts and non-experts was found.

Conclusion
The JES-BE classification system, including the diagnostic flowchart for predicting dysplastic BE, is acceptable and reliable, regardless of the clinician’s experience level.
Peri-anastomotic microdialysis lactate assessment after esophagectomy
Authors: Hedberg Jakob , Linder Gustav , Sundbom Magnus 
Published: July 2021

Abstract
Background
Esophagectomy is the cornerstone in curative treatment for esophageal and gastroesophageal junctional cancer. Esophageal resection is an advanced procedure with many complications, whereof anastomotic leak is the most dreaded. This study aimed to monitor the microcirculation with microdialysis analysis of local lactate levels in real-time on both sides of the esophagogastric anastomosis in totally minimally invasive Ivor-Lewis esophagectomy.

Materials and Methods
Twenty-five patients planned for esophageal resection with gastric conduit reconstruction and intrathoracic anastomosis were recruited. A sampling device, the OnZurf® Probe, along with the CliniSenz® Analyser (Senzime AB, Uppsala Sweden) was utilized for measurements. Lactate levels from both sides of the anastomosis were analysed in real time, on site, by a transportable analyser device. Measurements were made every 30 min during the first 24 h, and thereafter every 2 hours for up to 4 days.

Results
All probes could be positioned as planned and on the third postoperative day 19/25 and 15/25 of the esophageal and gastric probes, respectively, continued to deliver measurements. In total, 89.6% (1539/1718) and 72.4% (1098/1516) of the measurements were deemed successful. The average lactate level on the esophageal side of the anastomosis and the gastric conduit ranged between 1.1–11.5 and 0.8–7.0 mM, respectively. Two anastomotic leaks occurred, one of which had persisting high lactate levels on the gastric side of the anastomosis.

Conclusion
Application and use of the novel CliniSenz® analyser system, in combination with the OnZurf® Probe was feasible and safe. Continuous monitoring of analytes from the perianastomotic area has the potential to improve care after esophageal resection.
Construction and a preliminary study of paracrine effect of bone marrow-derived endothelial progenitor cell sheet
Authors: Xue Fenlong , Bai Yunpeng , Jiang Yiyao , Liu Jianshi , Jian Kaitao 
Published: July 2021

AbstractThe release of paracrine factors from endothelial progenitor cell (EPC) sheet is a central mechanism of tissue repair. The purpose of this study was to constuct the rat bone marrow derived-endothelial progenitor cell (BM-EPCs) sheet and investigate invest the role of stromal cell-derived factor-1α (SDF-1α)/CXCR4 axis in the biological function of BM-EPCs sheet. BM-EPC cells were identified by the cell-surface markers-CD34/CD133/VE-cadherin/KDR using flow cytometry and dual affinity for acLDL and UEA-1. After 7 days of incubation, the BM-EPC single-cell suspensions were seeded on thermo-sensitive plate to harvest the BM-EPC cell sheets. The expression levels of SDF-1α/CXCR4 axis-associated genes and proteins were examined using RT-qPCR and western blot analysis, and enzyme-linked immunosorbent assay (ELISA) was applied to determine the concentration of vascular endothelial growth factor (VEGF), epidermal growth factor (EGF) and SDF-1α in the cell culture medium. The BM-EPC cell sheets were successfully harvested. Moreover, BM-EPC cell sheets have superior migration and tube formation activity when compared with single cell suspension. When capillary-like tube were formed from EPCs sheets, the releasing of paracrine factors such as VEGF, EGF and SDF-1α were increased. To reveal the mechanism of tube formation of BM-EPCs sheets, our research showed that the activation of PI3K/AKT/eNOS pathway was involved in the process, because the phosphorylation of CXCR, PI3K, AKT and eNOS were increased. BM-EPC cell sheets have superior paracrine and tube formation activity than the BM-EPC single-cell. The strong ability to secrete paracrine factors was be potentially related to the SDF-1α/CXCR4 axis through PI3K/AKT/eNOS pathway.
Paralinguistic Features Communicated through Voice can Affect Appraisals of Confidence and Evaluative Judgments
Authors: Guyer Joshua J. , Briñol Pablo , Vaughan-Johnston Thomas I. , Fabrigar Leandre R. , Moreno Lorena , Petty Richard E. 
Published: July 2021

AbstractThis article unpacks the basic mechanisms by which paralinguistic features communicated through the voice can affect evaluative judgments and persuasion. Special emphasis is placed on exploring the rapidly emerging literature on vocal features linked to appraisals of confidence (e.g., vocal pitch, intonation, speech rate, loudness, etc.), and their subsequent impact on information processing and meta-cognitive processes of attitude change. The main goal of this review is to advance understanding of the different psychological processes by which paralinguistic markers of confidence can affect attitude change, specifying the conditions under which they are more likely to operate. In sum, we highlight the importance of considering basic mechanisms of attitude change to predict when and why appraisals of paralinguistic markers of confidence can lead to more or less persuasion.
A Survey on Pipelined FFT Hardware Architectures
Authors: Garrido Mario 
Published: July 2021

AbstractThe field of pipelined FFT hardware architectures has been studied during the last 50 years. This paper is a survey that includes the main advances in the field related to architectures for complex input data and power-of-two FFT sizes. Furthermore, the paper is intended to be educational, so that the reader can learn how the architectures work. Finally, the paper divides the architectures into serial and parallel. This classification puts together those architectures that are conceived for a similar purpose and, therefore, are comparable.
Untargeted fecal metabolome analysis in obese dogs after weight loss achieved by feeding a high-fiber-high-protein diet
Authors: Bermudez Sanchez Sandra , Pilla Rachel , Sarawichitr Benjamin , Gramenzi Alessandro , Marsilio Fulvio , Steiner Joerg M. , Lidbury Jonathan A. , Woods Georgiana R. T. , Suchodolski Jan S. , German Alexander J. 
Published: July 2021

Abstract
Introduction
In humans and companion animals, obesity is accompanied by metabolic derangements. Studies have revealed differences in the composition of the fecal microbiome between obese dogs and those with an ideal body weight.

Objectives
We have previously reported that the fecal microbiome in obese dogs changes after controlled weight reduction, induced by feeding a diet high in fiber and protein. Despite these findings, it is unclear if taxonomic differences infer differences at the functional level between obese dogs and those with an ideal body weight.

Methodology
Untargeted fecal metabolome analysis was performed on dogs with obesity before and after weight loss achieved by feeding a high-fiber-high-protein diet.

Results
Fecal metabolome analysis revealed a total of 13 compounds that changed in concentration in obese dogs after weight loss. Of these compounds, metabolites associated with bacterial metabolism decreased after weight loss including purine, L-(-)-methionine, coumestrol, and the alkaloids 1-methylxanthine and trigonelline. Conversely, the polyphenols (-)-epicatechin and matairesinol and the quinoline derivatives 1,5-isoquinolinediol and 2-hydroxiquinoline increased after weight loss.

Conclusion
These results suggest differences in intestinal microbiome at the functional level after weight loss, but further studies are needed to determine the role of these compounds in the etiology of obesity and weight loss.
Optimisation of the Synthesis and Cell Labelling Conditions for [89Zr]Zr-oxine and [89Zr]Zr-DFO-NCS: a Direct In Vitro Comparison in Cell Types with Distinct Therapeutic Applications
Authors: Friberger Ida , Jussing Emma , Han Jinming , Goos Jeroen A. C. M. , Siikanen Jonathan , Kaipe Helen , Lambert Mélanie , Harris Robert A. , Samén Erik , Carlsten Mattias , Holmin Staffan , Tran Thuy A. 
Published: July 2021

Abstract
Background
There is a need to better characterise cell-based therapies in preclinical models to help facilitate their translation to humans. Long-term high-resolution tracking of the cells in vivo is often impossible due to unreliable methods. Radiolabelling of cells has the advantage of being able to reveal cellular kinetics in vivo over time. This study aimed to optimise the synthesis of the radiotracers [89Zr]Zr-oxine (8-hydroxyquinoline) and [89Zr]Zr-DFO-NCS (p-SCN-Bn-Deferoxamine) and to perform a direct comparison of the cell labelling efficiency using these radiotracers.

Procedures
Several parameters, such as buffers, pH, labelling time and temperature, were investigated to optimise the synthesis of [89Zr]Zr-oxine and [89Zr]Zr-DFO-NCS in order to reach a radiochemical conversion (RCC) of >95 % without purification. Radio-instant thin-layer chromatography (iTLC) and radio high-performance liquid chromatography (radio-HPLC) were used to determine the RCC. Cells were labelled with [89Zr]Zr-oxine or [89Zr]Zr-DFO-NCS. The cellular retention of 89Zr and the labelling impact was determined by analysing the cellular functions, such as viability, proliferation, phagocytotic ability and phenotypic immunostaining.

Results
The optimised synthesis of [89Zr]Zr-oxine and [89Zr]Zr-DFO-NCS resulted in straightforward protocols not requiring additional purification. [89Zr]Zr-oxine and [89Zr]Zr-DFO-NCS were synthesised with an average RCC of 98.4 % (n = 16) and 98.0 % (n = 13), respectively. Cell labelling efficiencies were 63.9 % (n = 35) and 70.2 % (n = 30), respectively. 89Zr labelling neither significantly affected the cell viability (cell viability loss was in the range of 1–8 % compared to its corresponding non-labelled cells, P value > 0.05) nor the cells’ proliferation rate. The phenotype of human decidual stromal cells (hDSC) and phagocytic function of rat bone-marrow-derived macrophages (rMac) was somewhat affected by radiolabelling.

Conclusions
Our study demonstrates that [89Zr]Zr-oxine and [89Zr]Zr-DFO-NCS are equally effective in cell labelling. However, [89Zr]Zr-oxine was superior to [89Zr]Zr-DFO-NCS with regard to long-term stability, cellular retention, minimal variation between cell types and cell labelling efficiency.
Alkoholkonsum bei Medizinstudierenden. Eine Analyse über 7 Jahre (2011 bis 2017)
Authors: Jacobs Theresa , Linke Maike , Richter Ernst Peter , Drössler Stephanie , Zimmermann Anja , Berth Hendrik 
Published: July 2021

Zusammenfassung
Hintergrund
In der Literatur lassen sich Hinweise darauf finden, dass bei Medizinstudierenden häufig risikohafter Alkoholkonsum vorliegt.

Ziel der Arbeit
Das Ziel dieser Arbeit ist, den Alkoholkonsum Medizinstudierender im Zeitverlauf zu untersuchen.

Material und Methoden
Von 2011 bis 2017 wurden Dresdner Medizinstudierende des zweiten Semesters mit dem „Alcohol Use Disorders Identification Test“ (AUDIT) befragt. Mögliche beeinflussende Faktoren des Alkoholkonsums wie Alter, Geschlecht, psychische Belastung („Brief-Symptom Inventory-18“ [BSI-18]), Jahrgang und Abiturnote der Medizinstudierenden wurden in einer Regressionsanalyse geprüft.

Ergebnisse
Auffällige Scores im AUDIT wiesen 47 % der Studierenden auf. Die männlichen Studierenden zeigten verglichen mit ihren Kommilitoninnen signifikant höhere Scores (6,73 vs. 4,64; p < 0,001). Aus der Regressionsanalyse gingen das Geschlecht (p = 0,000) sowie die psychische Belastung (p = 0,041; Frauen: p = 0,000) als beeinflussende Faktoren des Alkoholkonsums hervor.

Schlussfolgerung
Die Medizinstudierenden des zweiten Semesters zeigen häufig problematischen Alkoholkonsum, wobei dies v. a. für die männlichen Studierenden gilt. Der Alkoholkonsum von Medizinstudierenden scheint sich von 2011 bis 2017 nicht zu verändern. Die psychische Belastung stand überwiegend bei den weiblichen Medizinstudierenden mit dem Alkoholkonsum in Zusammenhang.