bluuebunny/crossref_metadata_2025_split huggingface dataset:
A novel whole yeast-based subunit oral vaccine against Eimeria tenella in chickens
Authors: Soutter Francesca , Werling Dirk , Nolan Matthew , Küster Tatiana , Attree Elizabeth , Marugán-Hernández Virginia , Kim Sungwon , Tomley Fiona M. , Blake Damer P. 
Published: November 2021

AbstractCheap, easy-to-produce oral vaccines are needed for control of coccidiosis in chickens to reduce the impact of this disease on welfare and economic performance. Saccharomyces cerevisiae yeast expressing three Eimeria tenella antigens were developed and delivered as heat-killed, freeze-dried whole yeast oral vaccines to chickens in four separate studies. After vaccination, E. tenella replication was reduced following low dose challenge (250 oocysts) in Hy-Line Brown layer chickens (p<0.01). Similarly, caecal lesion score was reduced in Hy-Line Brown layer chickens vaccinated using a mixture of S. cerevisiae expressing EtAMA1, EtIMP1 and EtMIC3 following pathogenic-level challenge (4,000 E. tenella oocysts; p<0.01). Mean body weight gain post-challenge with 15,000 E. tenella oocysts was significantly increased in vaccinated Cobb500 broiler chickens compared to mock-vaccinated controls (p<0.01). Thus, inactivated recombinant yeast vaccines offer cost-effective and scalable opportunities for control of coccidiosis, with relevance to broiler production and chickens reared in low-and middle-income countries (LMICs).
Prediction of network public opinion based on bald eagle algorithm optimized radial basis function neural network
Authors: Xie Jialiang , Zhang Shanli , Lin Ling 
Published: November 2021

PurposeIn the new era of highly developed Internet information, the prediction of the development trend of network public opinion has a very important reference significance for monitoring and control of public opinion by relevant government departments.Design/methodology/approachAiming at the complex and nonlinear characteristics of the network public opinion, considering the accuracy and stability of the applicable model, a network public opinion prediction model based on the bald eagle algorithm optimized radial basis function neural network (BES-RBF) is proposed. Empirical research is conducted with Baidu indexes such as “COVID-19”, “Winter Olympic Games”, “The 100th Anniversary of the Founding of the Party” and “Aerospace” as samples of network public opinion.FindingsThe experimental results show that the model proposed in this paper can better describe the development trend of different network public opinion information, has good stability in predictive performance and can provide a good decision-making reference for government public opinion control departments.Originality/valueA method for optimizing the central value, weight, width and other parameters of the radial basis function neural network with the bald eagle algorithm is given, and it is applied to network public opinion trend prediction. The example verifies that the prediction algorithm has higher accuracy and better stability.
Numerical and experimental study on the static performance of externally pressurized thrust bearings lubricated with refrigerant gases
Authors: Rong Chengjun , Lian Huaqi , Li Yulong 
Published: November 2021

Purpose
Oil-free heat pumps that use the system refrigerant gases as lubricants are preferred for thermal management in future space applications. This study aims to numerically and experimentally investigate the static performance of externally pressurized thrust bearings lubricated with refrigerant gases.

Design/methodology/approach
The refrigerant gases R22, R410A and CO2 were chosen as the research objects, while N2 was used for comparison. Computational fluid dynamics was used to solve the full 3 D Navier–Stokes equations to determine the load capacity, static stiffness and static pressure distribution in the bearing film. The numerical results were experimentally verified.

Findings
The results showed that the refrigerant-gas-lubricated thrust bearings had a lower load capacity than the N2-lubricated bearings, but they presented a higher static stiffness when the bearing clearance was less than 9 µm. Compared with the N2-lubricated bearings, the optimal static stiffness of the R22- and CO2-lubricated bearings increased by more than 46% and more than 21%, respectively. The numerical and experimental results indicate that a small bearing clearance would be preferable when designing externally pressurized gas thrust bearings lubricated with the working medium of heat pump systems for space applications.

Originality/value
The findings of this study can serve as a basis for the further investigation of refrigerant gases as lubricants in heat pump systems, as well as for the future design of such gas bearings in heat pump systems for space applications.
The importance of the electrical properties of hydraulic fluids
Authors: Lovrec Darko , Tič Vito 
Published: November 2021

Purpose
Apart from the basic material properties of liquid lubricants, such as, e.g., the viscosity and density of the hydraulic fluid, it is also important to have information regarding the electrical properties of the fluid used. The latter is closely related to the purpose, type, structure, and conditions of use of a hydraulic system, especially the powertrain design and fluid condition monitoring. The insulating capacity of the hydraulic fluid is important in cases where the electric motor of the pump is immersed in the fluid. In other cases, on the basis of changing the electrical conductive properties of the hydraulic fluid, we can refer its condition, and, on this basis, the degree of degradation.

Design/methodology/approach
The paper first highlights the importance of knowing the electrical properties of hydraulic fluids and then aims to compare these properties, such as the breakdown voltage of commonly used hydraulic mineral oils and newer ionic fluids suitable for use as hydraulic fluids.

Findings
Knowledge of this property is crucial for the design approach of modern hydraulic compact power packs. In the following, the emphasis is on the more advanced use of known electrical quantities, such as electrical conductivity and the dielectric constant of a liquid.

Originality/value
Based on the changes in these quantities, we have the possibility of real-time monitoring the hydraulic fluid condition, on the basis of which we judge the degree of fluid degradation and its suitability for further use.
Toward a comprehensive scale of online shopping experiences: a mixed-method approach
Authors: Pentina Iryna , Zolfagharian Mohammadali , Michaud-Trevinal Aurélia 
Published: November 2021

PurposeThe objectives of the paper were to develop a theoretical framework of the online shopping experiences (OSE) concept, identify its constituent dimensions and subdimensions, and propose and test a comprehensive measurement scale that would incorporate and reconcile existing fragmented approaches to the phenomenon.Design/methodology/approachThis paper utilized a mixed-method approach to conceptualize a complex phenomenon of online shopping experiences (OSE). Study 1 employed the grounded theory approach to understand the phenomenon, propose its comprehensive definition and a conceptual model. Study 2 developed and tested a comprehensive OSE measurement scale.FindingsThe study conceptualized OSE as a second-order construct with four mutually-connected constituent dimensions of OSE: practices, context, values and emotions, and their respective 21 subdimensions. It developed and validated a comprehensive scale that is superior to earlier proposed fragmented measures of OSE.Research limitations/implicationsThe proposed theoretical framework can serve as a foundation for the OSE research stream and consolidate existing findings by providing a conceptual umbrella for different OSE aspects addressed in earlier research. The OSE measurement scale can be used to assess the impact of each OSE component on satisfaction and loyalty in different shopping situations.Practical implicationsThe findings can assist retailers in evaluating usefulness of various tools in eliciting certain experiential effects, devising specific approaches to consumers at different touch points along their dynamic OSEs and developing engagement tactics for various shopping environments.Originality/valueThe study combined qualitative and quantitative approaches to offer a most comprehensive and unifying conceptualization and measurement instrument of OSEs.
Establishing factors affecting the tax morale of individuals working in the gig economy
Authors: Md Fadzil Farah Nabila , Che Azmi Anna 
Published: November 2021

Purpose
The purpose of this study is to provide insights into the factors affecting the tax morale of workers in the gig economy. Tax morale is defined as the willingness and motivation to comply with tax laws.

Design/methodology/approach
Data was collected from gig economy workers through a questionnaire survey and analysed using second-generation multivariate analysis (partial least squares-structural equation modelling).

Findings
The findings reveal that while the extent of the dependency on the gig economy has a positive relationship with tax morale, the level of education has a negative relationship. However, in contrast to reflective moral attentiveness, perceptual moral attentiveness positively influence tax morale.

Originality/value
As no earlier study has examined factors affecting tax morale in the context of the gig economy, this research will be beneficial to tax authorities and policymakers. This study also offers insights into multidimensional aspects of the tax morale of those working in the gig economy.
A review of the legislative framework for social media records in Botswana
Authors: Mosweu Tshepho Lydia 
Published: November 2021

Purpose
The purpose of this paper to review the legislative framework for social media records in Botswana. The goal is to determine the extent to which the current legislation in Botswana covers the management of social media records.

Design/methodology/approach
This paper applied a qualitative research methodology and used documentary review method to collect data for analysis. The data collected was reviewed and organised into themes that cut across all the data sources to answer the main research objective.

Findings
The findings of this paper show that the Botswana Government has made strides in reforming some legislation to address issues that rise with the continued use of the internet and cloud services in the country. However, a review of the legislation established that the reforms were not comprehensive enough for records generated on social media. The relevant subsidiary legislation also fell shot in filing this gap in the country’s legislative framework.

Research limitations/implications
Research into the legislative framework for records generated on social media platforms in Botswana still needs more insights, specifically guidelines in the implementation of the current legislation in the country.

Practical implications
The findings of this paper can be used by both Botswana and other governments, especially in Africa where there is limited research in the area, to better manage records generated through the use of social media with respect to relevant legislative frameworks.

Originality/value
This paper is one of the first research contributions to review the legislative framework for records generated on social media in Botswana.
DebaryOmics: an integrative –omics study to understand the halophilic behaviour of Debaryomyces hansenii
Authors: Navarrete Clara , Sánchez Benjamín J. , Savickas Simonas , Martínez José L. 
Published: November 2021

SummaryDebaryomyces hansenii is a non‐conventional yeast considered to be a well‐suited option for a number of different industrial bioprocesses. It exhibits a set of beneficial traits (halotolerant, oleaginous, xerotolerant, inhibitory compounds resistant) which translates to a number of advantages for industrial fermentation setups when compared to traditional hosts. Although D. hansenii has been highly studied during the last three decades, especially in regards to its salt‐tolerant character, the molecular mechanisms underlying this natural tolerance should be further investigated in order to broadly use this yeast in biotechnological processes. In this work, we performed a series of chemostat cultivations in controlled bioreactors where D. hansenii (CBS 767) was grown in the presence of either 1M NaCl or KCl and studied the transcriptomic and (phospho)proteomic profiles. Our results show that sodium and potassium trigger different responses at both expression and regulation of protein activity levels and also complemented previous reports pointing to specific cellular processes as key players in halotolerance, moreover providing novel information about the specific genes involved in each process. The phosphoproteomic analysis, the first of this kind ever reported in D. hansenii, also implicated a novel and yet uncharacterized cation transporter in the response to high sodium concentrations.
Electrocardiogram evolution of acute anterior ST‐segment elevation myocardial infarction following pericarditis
Authors: Bai Xiaopeng , Wang Yufeng , Shi Shuai , Sun Lixiu , Zhao Jia , Liu Bingchen 
Published: November 2021

AbstractElectrocardiogram is a powerful tool for differentiating acute ST‐segment elevation myocardial infarction (STEMI) and pericarditis. However, an unusual ECG presentation of the simultaneous occurrence of the two conditions has not been reported previously. In this article, we report a case of ECG evolution of acute anterior STEMI following pericarditis with pericardial effusion (PE) and find that QRS complex widening in ECG lead with maximal ST‐segment elevation is also applicable for identifying STEMI even in patients with prior pericarditis. Undoubtedly, our case can help prevent emergency physicians from making incorrect diagnoses and administering inappropriate treatments.
Clinicopathological significance of CD28 overexpression in adult T‐cell leukemia/lymphoma
Authors: Sakamoto Yuma , Ishida Takashi , Masaki Ayako , Takeshita Morishige , Iwasaki Hiromi , Yonekura Kentaro , Tashiro Yukie , Ito Asahi , Kusumoto Shigeru , Iida Shinsuke , Utsunomiya Atae , Ueda Ryuzo , Inagaki Hiroshi 
Published: November 2021

AbstractCD28, one of the costimulatory molecules, has a pivotal role in T‐cell activation, and its expression is strictly regulated in normal T cells. Gain‐of‐function genetic alterations involving CD28 have been frequently observed in adult T‐cell leukemia/lymphoma (ATLL). These abnormalities, such as CD28 fusions and copy number variations, may not only confer continuous, prolonged, and enhanced CD28 signaling to downstream pathways but also induce overexpression of the CD28 protein. In this study, 120 ATLL cases were examined by immunohistochemistry for CD28 and its ligands CD80 and CD86, and their expression on tumor cells was semiquantitatively evaluated. CD28 was overexpressed in 55 (46%) cases, and CD80 or CD86 (CD80/CD86) was infrequently overexpressed in 12 (11%). Compared with non‐overexpressers, CD28 overexpressers showed a higher frequency of CD28 genetic alterations and had an increased number of CD80/CD86‐positive non‐neoplastic cells infiltrating tumor microenvironment. In the entire ATLL patient cohort, CD28 overexpressers showed a significantly poorer overall survival (OS) compared with non‐overexpressers (P = .001). The same was true for a subgroup who were treated with multidrug regimens with or without mogamulizumab. CD28 overexpression had no prognostic impact in the group who received allogeneic hematopoietic stem cell transplantation. In the multivariate analysis for OS, CD28 overexpression was selected as an independent risk factor. These results suggest ATLL patients with CD28 overexpression have more aggressive clinical course and are more refractory to treatment with multidrug chemotherapy. CD28 overexpression appears to be a novel unfavorable prognostic marker in ATLL patients, and further prospective studies are warranted to establish its prognostic significance.
Microstructural white matter abnormalities in Lesch–Nyhan disease
Authors: Del Bene Victor A. , Crawford Jeffrey L. , Gómez‐Gastiasoro Ainara , Vannorsdall Tracy D. , Buchholz Alison , Ojeda Natalia , Harris James C. , Jinnah Hyder A. , Schretlen David J. 
Published: November 2021

AbstractLesch–Nyhan disease is a rare, sex‐linked, genetic neurodevelopmental disorder that is characterized by hyperuricemia, dystonia, cognitive impairment and recurrent self‐injury. We previously found reduced brain white matter volume in patients with Lesch–Nyhan disease compared with healthy adults using voxel‐based morphometry. Here, we address the structural integrity of white matter via diffusion tensor imaging. We hypothesized that white matter integrity would be decreased in men with Lesch–Nyhan disease and to a lesser extent in men with a milder variant of the disease (Lesch–Nyhan variant) relative to healthy men. After acquiring diffusion‐weighted brain images from Lesch–Nyhan disease (n = 5), Lesch–Nyhan variant (n = 6) and healthy participants (n = 10), we used both tract‐based spatial statistics and a regions of interest approach to analyse between‐group fractional anisotropy differences. We first replicated earlier findings of reduced intracranial, grey matter and white matter volumes in patients. We then discovered marked reductions of fractional anisotropy relative to the healthy control group. The Lesch–Nyhan disease group showed more pronounced reductions in white matter integrity than the Lesch–Nyhan variant group. In addition to whole brain fractional anisotropy group differences, reductions in white matter integrity were observed in the corpus callosum, corona radiata, cingulum, internal capsule and superior longitudinal fasciculus. Moreover, the variant group had attenuated dystonia severity symptoms and cognitive deficits. These findings highlight the need to better understand the role of white matter in Lesch–Nyhan disease.
Do predators keep prey healthy or make them sicker? A meta‐analysis
Authors: Richards Robert L. , Drake John M. , Ezenwa Vanessa O. 
Published: November 2021

AbstractEcological theory suggests that predators can either keep prey populations healthy by reducing parasite burdens or alternatively, increase parasitism in prey. To quantify the overall magnitude and direction of the effect of predation on parasitism in prey observed in practice, we conducted a meta‐analysis of 47 empirical studies. We also examined how study attributes, including parasite type and life cycle, habitat type, study design, and whether predators were able to directly consume prey contributed to variation in the predator‐prey‐parasite interaction. We found that the overall effect of predation on parasitism differed between parasites and parasitoids and that whether consumptive effects were present, and whether a predator was a non‐host spreader of parasites, were the most important traits predicting the parasite response. Our results suggest that the mechanistic basis of predator‐prey interactions strongly influences the effects of predators on parasites and that these effects, although context dependent, are predictable.
Effects of performance goal orientations on learning performance and in‐game performance in digital game‐based learning
Authors: Yang Jie Chi , Chung Ching‐Jung , Chen Mei‐Shan 
Published: November 2021

AbstractBackgroundPerformance goal orientations are influential motivational factors for predicting learning performance. However, a lack of attention has been paid to investigating the effects of performance goal orientations on learning performance and in‐game performance in the context of digital game‐based learning.ObjectivesThis study implemented a game‐based English vocabulary learning system and investigated how performance goal orientations affected learners' learning performance and in‐game performance.MethodsA total of 59 non‐English major university students participated in this study, divided into performance‐approach learners (PAPL) and performance‐avoidance learners (PAVL) based on their performance goal orientations. The English vocabulary listening test and log data were collected for conducting quantitative analyses.Results and conclusionsThe results showed that the PAPL outperformed the PAVL in learning performance, whereas the two groups demonstrated similar gain scores and overall in‐game performance. Moreover, the results showed that the PAVL's learning performance was strongly associated with all aspects of in‐game performance in the learning activities. Conversely, the PAPL's learning performance was only associated with two aspects of in‐game performance in the competition activities.ImplicationsThe findings of this study suggest that performance goal orientations play significant roles in both learning performance and in‐game performance, which could benefit the PAVL's in‐game performance, which is a substantial factor in promoting their learning performance via DGBL.
Mechanism of improving interfacial hydration characteristic of high‐denatured peanut protein induced by cold plasma
Authors: Yu Jiao‐jiao , Jiang Pei‐yun , Li Shu‐hong , Chen Ye 
Published: November 2021

AbstractHigh‐denatured peanut protein (HPP), obtained from high‐temperature pressed peanut meal, is limited in the food industry applications due to poor hydration characteristics. The ionic force generated by the cold plasma (CP) was used to activate the surface groups of HPP for generating the interfacial chemical force binding with water molecules, in which the high‐speed shear homogenization (12,000 r/min, 15 min) was as a pretreatment to enhance the accessibility for CP. The content of soluble protein increased from 0.22 ± 0.005 to 1.02 ± 0.03 mg/ml with a CP treatment power of 70 W for 2 min. Meanwhile, CP treatment facilitated the transition of weakly bound water to strongly bound water, therefore, the hydration property was improved. The tertiary, secondary, and apparent structure in HPP stretched after CP treatment. Besides, NH4+ ions in CP excited state improved the flexibility of HPP. Some hydrophilic groups such as COOH, CO, C═O, and OH formed via hydrogen bond to HPP surface was the primary reason for the increased hydration property, which was related to an increase of oxygen on the surface of protein. The increased interfacial hydration property of proteins provides the possibility for the preparation of preparation of functional films, gels, and nutrition agents.Practical applicationsCold plasma (CP), as a nonthermal and surface processing technology, has drawn great attention during the processing. The amount of free radicals, high‐energy electrons, ions, and ultraviolet radiation generated by CP modified the surface structure of the materials, which avoided thermal denaturation and pollution, and had high efficiency. This study indicated that CP treatment enhanced the interfacial hydration characteristic of the high‐denatured peanut protein extracted from peanut meal via analyzing the changes of structure, functional property, as well as chemical bonds, which was beneficial for the preparation of functional films, and gels for drug and nutrition delivery.