bluuebunny/crossref_metadata_2025_split huggingface dataset:
Exploring the Impacts of Source Condition Uncertainties on Far‐Field Brine Leakage Plume Predictions in Geologic Storage of CO2: Integrating Intermediate‐Scale Laboratory Testing With Numerical Modeling
Authors: Askar Ahmad H. , Illangasekare Tissa H. , Trautz Andrew , Solovský Jakub , Zhang Ye , Fučík Radek 
Published: August 2021

AbstractNatural fissures/faults or pressure‐induced fractures in the caprock confining injected CO2 have been identified as a potential leakage pathways of far‐field native brine contaminating underground sources of drinking water. Developing models to simulate brine propagation through the overlaying formations and aquifers is essential to conduct reliable pre‐ and post‐risk assessments for site selection and operation, respectively. One of the primary challenges of performing such simulations is lack of adequate information about source conditions, such as hydro‐structural properties of caprock fracture/fault zone and the permeability field of the storage formation. This research investigates the impact of source condition uncertainties on the accuracy of leaking brine plume predictions. Prediction models should be able to simulate brine leakage and transport in complex multilayered geologic systems with interacting regional natural and leakage flows. As field datasets are not readily available for model testing and validation, three comprehensive intermediate‐scale laboratory experiments were used to generate high‐resolution spatiotemporal data on brine plume development under different leakage scenarios. Experimental data were used to validate a flow and transport model developed using existing code FEFLOW to simulate brine plume under varying source conditions. Spatial moment analysis was conducted to evaluate how uncertainty in source conditions impacts brine migration predictions. Results showed that inaccurately prescribing the permeability field of storage formation and caprock fractures in models can cause errors in leakage pathway and spread predictions up to ∼19% and ∼100%, respectively. These findings will help in selecting and characterizing storage sites by factoring in potential risks to shallow groundwater resources.
NOTCH1 signaling promotes protein stability of HER3 through the AKT pathway in squamous cell carcinoma of head and neck
Authors: Wang Yi-Ping , Liu I-Ju , Chen Kai-Chi , Wu Han-Chung 
Published: August 2021

AbstractEpidermal growth factor receptor (EGFR) remains the sole druggable molecular target other than the PD1/PD-L1 pathway with meaningful clinical benefit in squamous cell carcinoma of head and neck (SCCHN). Human epidermal growth factor receptor 3 (HER3) confers the resistance to EGFR-targeted treatment in SCCHN. Thus, it is essential to determine the distribution and regulatory mechanisms of HER3 in SCCHN. We explored the prevalence of HER3 expression and its distribution within SCCHN by immunohistochemical staining and clinicopathological correlations were analyzed. The regulatory mechanism of HER3 expression was then dissected in vitro, using RT-PCR, Western blotting, and immunoprecipitation in a set of SCCHN cell lines. Subsequent in vivo validation in the murine model was also performed. We found that concomitant high expression of HER3 and its ligand NRG1 in SCCHN is associated with the increased presence of regional lymphatic metastasis and the majority of HER3 is located on the differentiated tumor cells. Further investigation revealed that HER3 is under positive control of NOTCH1 through transcriptional activation and inhibition of protein degradation through the polyubiquitination machinery via AKT pathway and USP8 deubiquitinating enzyme. In addition, loss of function of NOTCH1 suppresses HER3 expression through increased phosphorylation of serine 473 of AKT in SCCHN cells, and promotes the aggressiveness of the tumor cells. These data indicated that the level of HER3 is regulated by NOTCH1 in SCCHN both transcriptionally and post-translationally, and NOTCH1 is in a higher hierarchy in the regulatory system of the AKT pathway. Since NOTCH1 is inactivated in approximately 10% of SCCHN cases and this aberration strongly impacts the AKT pathway and diminishes HER3, exclusion of patients with NOTCH1-inactivated SCCHN may be beneficial for future clinical trials of HER3-targeting antibodies.
The DELUX study: development of lung volumes during extubation of preterm infants
Authors: Plastina Leonie , Gaertner Vincent D. , Waldmann Andreas D. , Thomann Janine , Bassler Dirk , Rüegger Christoph M. 
Published: August 2021

Abstract
Objective
To measure changes in end-expiratory lung impedance (EELI) as a marker of functional residual capacity (FRC) during the entire extubation procedure of very preterm infants.

Methods
Prospective observational study in preterm infants born at 26–32 weeks gestation being extubated to non-invasive respiratory support. Changes in EELI and cardiorespiratory parameters (heart rate, oxygen saturation) were recorded at pre-specified events during the extubation procedure compared to baseline (before first handling of the infant).

Results
Overall, 2912 breaths were analysed in 12 infants. There was a global change in EELI during the extubation procedure (p = 0.029). EELI was lowest at the time of extubation [median (IQR) difference to baseline: −0.30 AU/kg (−0.46; −0.14), corresponding to an FRC loss of 10.2 ml/kg (4.8; 15.9), padj = 0.004]. The biggest EELI loss occurred during adhesive tape removal [median change (IQR): −0.18 AU/kg (−0.22; −0.07), padj = 0.004]. EELI changes were highly correlated with changes in the SpO2/FiO2 ratio (r = 0.48, p < 0.001). Forty per cent of FRC was re-recruited at the tenth breath after the initiation of non-invasive ventilation (p < 0.001).

Conclusions
The extubation procedure is associated with significant changes in FRC. This study provides novel information for determining the optimal way of extubating a preterm infant.

Impact

This study is the first to examine the development of lung volumes during the entire extubation procedure including the impact of associated events.

The extubation procedure significantly affects functional residual capacity with a loss of approximately 10 ml/kg at the time of extubation.

Removal of adhesive tape is the major contributing factor to FRC loss during the extubation procedure.

Functional residual capacity is regained within the first breaths after initiation of non-invasive ventilation and is further increased after turning the infant into the prone position.
Genome-wide association study of COVID-19 severity among the Chinese population
Authors: Li Yuanfeng , Ke Yuehua , Xia Xinyi , Wang Yahui , Cheng Fanjun , Liu Xinyi , Jin Xin , Li Boan , Xie Chengyong , Liu Siyang , Chen Weijun , Yang Chenning , Niu Yuguang , Jia Ruizhong , Chen Yong , Liu Xiong , Wang Zhihua , Zheng Fang , Jin Yan , Li Zhen , Yang Ning , Cao Pengbo , Chen Hongxia , Ping Jie , He Fuchu , Wang Changjun , Zhou Gangqiao 
Published: August 2021

AbstractSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection causes a broad clinical spectrum of coronavirus disease 2019 (COVID-19). The development of COVID-19 may be the result of a complex interaction between the microbial, environmental, and host genetic components. To reveal genetic determinants of susceptibility to COVID-19 severity in the Chinese population, we performed a genome-wide association study on 885 severe or critical COVID-19 patients (cases) and 546 mild or moderate patients (controls) from two hospitals, Huoshenshan and Union hospitals at Wuhan city in China. We identified two loci on chromosome 11q23.3 and 11q14.2, which are significantly associated with the COVID-19 severity in the meta-analyses of the two cohorts (index rs1712779: odds ratio [OR] = 0.49; 95% confidence interval [CI], 0.38–0.63 for T allele; P = 1.38 × 10−8; and index rs10831496: OR = 1.66; 95% CI, 1.38–1.98 for A allele; P = 4.04 × 10−8, respectively). The results for rs1712779 were validated in other two small COVID-19 cohorts in the Asian populations (P = 0.029 and 0.031, respectively). Furthermore, we identified significant eQTL associations for REXO2, C11orf71, NNMT, and CADM1 at 11q23.3, and CTSC at 11q14.2, respectively. In conclusion, our findings highlight two loci at 11q23.3 and 11q14.2 conferring susceptibility to the severity of COVID-19, which might provide novel insights into the pathogenesis and clinical treatment of this disease.
Preliminary demonstration of a persistent Josephson phase-slip memory cell with topological protection
Authors: Ligato Nadia , Strambini Elia , Paolucci Federico , Giazotto Francesco 
Published: August 2021

AbstractSuperconducting computing promises enhanced computational power in both classical and quantum approaches. Yet, scalable and fast superconducting memories are not implemented. Here, we propose a fully superconducting memory cell based on the hysteretic phase-slip transition existing in long aluminum nanowire Josephson junctions. Embraced by a superconducting ring, the memory cell codifies the logic state in the direction of the circulating persistent current, as commonly defined in flux-based superconducting memories. But, unlike the latter, the hysteresis here is a consequence of the phase-slip occurring in the long weak link and associated to the topological transition of its superconducting gap. This disentangles our memory scheme from the large-inductance constraint, thus enabling its miniaturization. Moreover, the strong activation energy for phase-slip nucleation provides a robust topological protection against stochastic phase-slips and magnetic-flux noise. These properties make the Josephson phase-slip memory a promising solution for advanced superconducting classical logic architectures or flux qubits.
Exosomal miR-19a and IBSP cooperate to induce osteolytic bone metastasis of estrogen receptor-positive breast cancer
Authors: Wu Kerui , Feng Jiamei , Lyu Feng , Xing Fei , Sharma Sambad , Liu Yin , Wu Shih-Ying , Zhao Dan , Tyagi Abhishek , Deshpande Ravindra Pramod , Pei Xinhong , Ruiz Marco Gabril , Takahashi Hiroyuki , Tsuzuki Shunsuke , Kimura Takahiro , Mo Yin-yuan , Shiozawa Yusuke , Singh Ravi , Watabe Kounosuke 
Published: August 2021

AbstractBone metastasis is an incurable complication of breast cancer. In advanced stages, patients with estrogen-positive tumors experience a significantly higher incidence of bone metastasis (>87%) compared to estrogen-negative patients (<56%). To understand the mechanism of this bone-tropism of ER+ tumor, and to identify liquid biopsy biomarkers for patients with high risk of bone metastasis, the secreted extracellular vesicles and cytokines from bone-tropic breast cancer cells are examined in this study. Both exosomal miR-19a and Integrin-Binding Sialoprotein (IBSP) are found to be significantly upregulated and secreted from bone-tropic ER+ breast cancer cells, increasing their levels in the circulation of patients. IBSP is found to attract osteoclast cells and create an osteoclast-enriched environment in the bone, assisting the delivery of exosomal miR-19a to osteoclast to induce osteoclastogenesis. Our findings reveal a mechanism by which ER+ breast cancer cells create a microenvironment favorable for colonization in the bone. These two secreted factors can also serve as effective biomarkers for ER+ breast cancer to predict their risks of bone metastasis. Furthermore, our screening of a natural compound library identifies chlorogenic acid as a potent inhibitor for IBSP-receptor binding to suppress bone metastasis of ER+ tumor, suggesting its preventive use for bone recurrence in ER+ patients.
More than a biomarker: could language be a biosocial marker of psychosis?
Authors: Palaniyappan Lena 
Published: August 2021

AbstractAutomated extraction of quantitative linguistic features has the potential to predict objectively the onset and progression of psychosis. These linguistic variables are often considered to be biomarkers, with a large emphasis placed on the pathological aberrations in the biological processes that underwrite the faculty of language in psychosis. This perspective offers a reminder that human language is primarily a social device that is biologically implemented. As such, linguistic aberrations in patients with psychosis reflect both social and biological processes affecting an individual. Failure to consider the sociolinguistic aspects of NLP measures will limit their usefulness as digital tools in clinical settings. In the context of psychosis, considering language as a biosocial marker could lead to less biased and more accessible tools for patient-specific predictions in the clinic.
Phase-amplitude coupling between infraslow and high-frequency activities well discriminates between the preictal and interictal states
Authors: Hashimoto Hiroaki , Khoo Hui Ming , Yanagisawa Takufumi , Tani Naoki , Oshino Satoru , Kishima Haruhiko , Hirata Masayuki 
Published: August 2021

AbstractInfraslow activity (ISA) and high-frequency activity (HFA) are key biomarkers for studying epileptic seizures. We aimed to elucidate the relationship between ISA and HFA around seizure onset. We enrolled seven patients with drug-resistant focal epilepsy who underwent intracranial electrode placement. We comparatively analyzed the ISA, HFA, and ISA-HFA phase-amplitude coupling (PAC) in the seizure onset zone (SOZ) or non-SOZ (nSOZ) in the interictal, preictal, and ictal states. We recorded 15 seizures. HFA and ISA were larger in the ictal states than in the interictal or preictal state. During seizures, the HFA and ISA of the SOZ were larger and occurred earlier than those of nSOZ. In the preictal state, the ISA-HFA PAC of the SOZ was larger than that of the interictal state, and it began increasing at approximately 87 s before the seizure onset. The receiver-operating characteristic curve revealed that the ISA-HFA PAC of the SOZ showed the highest discrimination performance in the preictal and interictal states, with an area under the curve of 0.926. This study demonstrated the novel insight that ISA-HFA PAC increases before the onset of seizures. Our findings indicate that ISA-HFA PAC could be a useful biomarker for discriminating between the preictal and interictal states.
Stronger connectivity and higher extraversion protect against stress-related deterioration of cognitive functions
Authors: Rogala Jacek , Dreszer Joanna , Malinowska Urszula , Waligóra Marek , Pluta Agnieszka , Antonova Ingrida , Wróbel Andrzej 
Published: August 2021

AbstractHere we attempted to define the relationship between: EEG activity, personality and coping during lockdown. We were in a unique situation since the COVID-19 outbreak interrupted our independent longitudinal study. We already collected a significant amount of data before lockdown. During lockdown, a subgroup of participants willingly continued their engagement in the study. These circumstances provided us with an opportunity to examine the relationship between personality/cognition and brain rhythms in individuals who continued their engagement during lockdown compared to control data collected well before pandemic. The testing consisted of a one-time assessment of personality dimensions and two sessions of EEG recording and deductive reasoning task. Participants were divided into groups based on the time they completed the second session: before or during the COVID-19 outbreak ‘Pre-pandemic Controls’ and ‘Pandemics’, respectively. The Pandemics were characterized by a higher extraversion and stronger connectivity, compared to Pre-pandemic Controls. Furthermore, the Pandemics improved their cognitive performance under long-term stress as compared to the Pre-Pandemic Controls matched for personality traits to the Pandemics. The Pandemics were also characterized by increased EEG connectivity during lockdown. We posit that stronger EEG connectivity and higher extraversion could act as a defense mechanism against stress-related deterioration of cognitive functions.
Assessment of global hydro-social indicators in water resources management
Authors: Bozorg-Haddad Omid , Baghban Sahar , Loáiciga Hugo A. 
Published: August 2021

AbstractWater is a vital element that plays a central role in human life. This study assesses the status of indicators based on water resources availability relying on hydro-social analysis. The assessment involves countries exhibiting decreasing trends in per capita renewable water during 2005–2017. Africa, America, Asia, Europe, and Oceania encompass respectively 48, 35, 43, 20, and 5 countries with distinct climatic conditions. Four hydro-social indicators associated with rural society, urban society, technology and communication, and knowledge were estimated with soft-computing methods [i.e., artificial neural networks, adaptive neuro-fuzzy inference system, and gene expression programming (GEP)] for the world’s continents. The GEP model’s performance was the best among the computing methods in estimating hydro-social indicators for all the world’s continents based on statistical criteria [correlation coefficient (R), root mean square error (RMSE), and mean absolute error]. The values of RMSE for GEP models for the ratio of rural to urban population (PRUP), population density, number of internet users and education index parameters equaled (0.084, 0.029, 0.178, 0.135), (0.197, 0.056, 0.152, 0.163), (0.151, 0.036, 0.123, 0.210), (0.182, 0.039, 0.148, 0.204) and (0.141, 0.030, 0.226, 0.082) for Africa, America, Asia, Europe and Oceania, respectively. Scalable equations for hydro-social indicators are developed with applicability at variable spatial and temporal scales worldwide. This paper’s results show the patterns of association between social parameters and water resources vary across continents. This study’s findings contribute to improving water-resources planning and management considering hydro-social indicators.
A well-trained artificial neural network for predicting the rheological behavior of MWCNT–Al2O3 (30–70%)/oil SAE40 hybrid nanofluid
Authors: Esfe Mohammad Hemmat , Eftekhari S. Ali , Hekmatifar Maboud , Toghraie Davood 
Published: August 2021

AbstractIn this study, the influence of different volume fractions ($$\phi$$ϕ) of nanoparticles and temperatures on the dynamic viscosity ($$\mu_{nf}$$μnf) of MWCNT–Al2O3(30–70%)/oil SAE40 hybrid nanofluid was examined by ANN. For this reason, the$$\mu_{nf}$$μnfwas derived for 203 various experiments through a series of experimental tests, including a combination of 7 different$$\phi$$ϕ, 6 various temperatures, and 5 shear rates. These data were then used to train an artificial neural network (ANN) to generalize results in the predefined ranges for two input parameters. For this reason, a feed-forward perceptron ANN with two inputs (T and$$\phi$$ϕ) and one output ($$\mu_{nf}$$μnf) was used. The best topology of the ANN was determined by trial and error, and a two-layer with 10 neurons in the hidden layer with the tansig function had the best performance. A well-trained ANN is created using thetrainbralgorithm and showed an MSE value of 4.3e−3 along 0.999 as a correlation coefficient for predicting$$\mu_{nf}$$μnf. The results show that an increase$$\phi$$ϕhas a significant effect on$$\mu_{nf}$$μnfvalue. As$$\phi$$ϕincreases, the viscosity of this nanofluid increases at all temperatures. On the other hand, with increasing temperature, the viscosity of this nanofluid decreases. Based on all of the diagrams presented for the trained ANNs, we can conclude that a well-trained ANN can be used as an approximating function for predicting the$$\mu_{nf}$$μnf.
Physical performance in relation to preserved ratio impaired spirometry: a cross-sectional study of community-dwelling older Japanese adults
Authors: Anami Kunihiko , Murata Shin , Nakano Hideki , Nonaka Koji , Iwase Hiroaki , Shiraiwa Kayoko , Abiko Teppei , Goda Akio , Horie Jun 
Published: August 2021

AbstractPreserved ratio impaired spirometry (PRISm) is associated with an increased mortality rate; however, its characteristics have not been clearly identified in Japan. This cross-sectional study of community-dwelling older adults compared physical function between people with PRISm and those with no respiratory issues, from 2014 to 2019. We collected demographic data through interviews and measured respiratory and physical functions. We included 668 older adults (male, 23.5%; mean age, 72.8 ± 5.6 years); the prevalence of PRISm was 12%, while the prevalence of obstruction was 6.9%. Propensity score matching was used to identify control subjects with normal spirometry (n = 80) while minimizing the effects of confounders during comparisons with the PRISm population (n = 80). Compared with community-dwelling older adults with normal lung capacity, older adults with PRISm had a lower forced vital capacity (%FVC; 68.7 ± 9.1% vs. 92.5 ± 12.7%, p < 0.001), lower core muscle endurance (sit-up test: 6.7 ± 5.8 vs. 8.7 ± 6.0, p = 0.032), and a longer one-leg stance duration (52.4 ± 41.1 s vs. 36.4 ± 34.1 s, p = 0.008). In multivariable logistic regression, %FVC and increased one-leg stance were independent predictors of PRISm status. The prevalence of PRISm among community-dwelling elderly Japanese exceeds that of obstructive lung disease and is associated with reduced %FVC and better performance on balance testing.
Quantitative X-ray tomographic analysis reveals calcium precipitation in cataractogenesis
Authors: Li Yuxing , Parkinson Dilworth Y. , Feng Jun , Xia Chun-hong , Gong Xiaohua 
Published: August 2021

AbstractCataracts, named for pathological light scattering in the lens, are known to be associated with increased large protein aggregates, disrupted protein phase separation, and/or osmotic imbalances in lens cells. We have applied synchrotron phase contrast X-ray micro-computed tomography to directly examine an age-related nuclear cataract model in Cx46 knockout (Cx46KO) mice. High-resolution 3D X-ray tomographic images reveal amorphous spots and strip-like dense matter precipitates in lens cores of all examined Cx46KO mice at different ages. The precipitates are predominantly accumulated in the anterior suture regions of lens cores, and they become longer and dense as mice age. Alizarin red staining data confirms the presence of calcium precipitates in lens cores of all Cx46KO mice. This study indicates that the spatial and temporal calcium precipitation is an age-related event associated with age-related nuclear cataract formation in Cx46KO mice, and further suggests that the loss of Cx46 promotes calcium precipitates in the lens core, which is a new mechanism that likely contributes to the pathological light scattering in this age-related cataract model.
Polyclonal alpaca antibodies protect against hantavirus pulmonary syndrome in a lethal Syrian hamster model
Authors: Sroga Patrycja , Sloan Angela , Warner Bryce M. , Tierney Kevin , Lew Jocelyne , Liu Guodong , Chan Michael , Deschambault Yvon , Stein Derek R. , Soule Geoff , Banadyga Logan , Falzarano Darryl , Safronetz David 
Published: August 2021

AbstractThe use of antibody-based therapies for the treatment of high consequence viral pathogens has gained interest over the last fifteen years. Here, we sought to evaluate the use of unique camelid-based IgG antibodies to prevent lethal hantavirus pulmonary syndrome (HPS) in Syrian hamsters. Using purified, polyclonal IgG antibodies generated in DNA-immunized alpacas, we demonstrate that post-exposure treatments reduced viral burdens and organ-specific pathology associated with lethal HPS. Antibody treated animals did not exhibit signs of disease and were completely protected. The unique structures and properties, particularly the reduced size, distinct paratope formation and increased solubility of camelid antibodies, in combination with this study support further pre-clinical evaluation of heavy-chain only antibodies for treatment of severe respiratory diseases, including HPS.