bluuebunny/crossref_metadata_2025_split huggingface dataset:
Selected Micro RNAs Regulate Drug Resistance Genes in Breast Cancer.
Authors: Zaib Shumaila , Yasmin Azra , Masood Nosheen 
Published: June 2021

Abstract
Background: Breast cancer initiation is an unresolved phenomenon although many genes are known to be involved in its initiation but its exact etiology is still unexplained in many aspects and recently microRNAs are found to regulate many genes expressions. Method: This case control study has been designed to evaluate the role of selected miRNAs in gene expression and subsequent association with drug resistance. Genetic polymorphisms were confirmed by PCR-SSCP followed by sequencing and microRNA expression was measured by realtime PCR with specific primers. Follow up was done for patients whose samples were used in the study. Results: This study revealed 15 germline mutations in mdr1, 5 in ABCG2, 8 in BRCA1 and 8 in PTEN gene. These mutations were significantly associated with breast cancer compared with control tissues (P<0.05). miR-21, miR-146a and miR-328 were over expressed whereas miR-451 was under expressed. Progression free survival (PFS) was linked with reduced polymorphisms in genes as well as microRNAs. Conclusion: Over expression of miRNA-21 and miRNA-146a may lead towards lower expression of PTEN and BRCA1 genes causing cancer progression. miRNA-328 and miRNA-451 reduced expression resulted in overexpressed ABCG2 and MDR1 genes in this study.
WITHDRAWN: Evaluation of Polarimetric Eigenvector Based Decomposition for Types of Wetland Mapping
Authors: Adhikari Ruma 
Published: June 2021

Abstract
ContextThe Kosi mega fan landscape is an alluvial deposited site that have led to several devastating floods. A considerable portion of the land comprises of different types of wetlands and persistent surface wetness. Detection of these wet patches is important in terms of understanding morphology of this sediment deposited fan.ObjectivesThis study identifies different wetland patches in monsoon time with critical reaches of rail-road and waterlogged intersections.MethodsSAR Polarimetry based H-A-Alpha decomposition and Wishart classification is used to identify different backscatter intensities of vegetated wetland, wet soil and open water using dual-pol Sentinel-1 10m data.ResultsThis study showed that Wishart classification offers a good potential for herbaceous waterbody mapping. The presence of a varieties types of wetlands is identified based on their scattering properties. Preliminary it has identified wet soil, low land, marshy patches as well as open water. Though at some places it mistakenly classified vegetation covered water as forest land. Some dry soil also exhibits low entropy volume scattering which is similar to certain type of vegetation cover. This study also identifies the vulnerable zone of waterlogging to existing rail and road network.
Complement 4 Aids in Prediction of Newly Diagnosed Multiple Myeloma Outcome in Patients
Authors: Zhang Lihua , Ling Xiaosui , Li Fan , Yang Tonghua , Shi Keqian , Zhao Shixiang , Yu Liqun , Li Zengzheng , He Haiping 
Published: June 2021

Abstract
Background and aim: A cure for the heterogeneous hematological malignancy Multiple Myeloma (MM) is yet to be developed. To date, the early risk factors associated with poor outcomes in MM have not been fully elucidated. Studies have shown an aberrant complement system in MM patients, but the precise association necessitates elucidation. Therefore, this study scrutinizes the correlation between serum complement level and the disease outcome of MM patients.Materials and methods: A retrospective analysis of 72 MM patients (new diagnosis) with complement C4 and C3 along with common laboratory indicators was done. The Pearson’s χ2 test and the Mann–Whitney U test were done to evaluate categorical or binary variables and inter-group variance, respectively. Kaplan-Meier test and Cox’s proportional hazards regression were employed for quantitation of overall survival (OS) and univariate or multivariate analyses, respectively.Results: The Cox proportional hazard model analysis unveiled the following: platelet≤115.5×10^9/L(HR=5.82，95%CI=2.522-13.436, P<0.001)， complement C4≤0.095g/L(HR=3.642, 95%CI=1.486-8.924,P=0.005), age≥67 years(HR=0.191, 95%CI=0.078-0.47, P<0.001)，bone marrow plasma cell percentage≥30.75% (HR=0.171, 95%CI=0.06-0.482, P=0.001) can be employed as independent predictors of OS. Of these, advanced age, low platelet level, and a high proportion of bone marrow plasma cells have been implicated in poor outcomes in MM patients. Interestingly, a low complement 4 level can function as a new indicator of poor prognosis in MM patients.Conclusion: Low levels of C4 are indicative of a poor outcome in newly diagnosed MM patients.
Chaotic Fitness Dependent Optimizer for Planning and Engineering Design
Authors: Mohammed Hardi M. , Rashid Tarik A. 
Published: June 2021

Abstract
Fitness Dependent Optimizer (FDO) is a recent metaheuristic algorithm that mimics the reproduction behavior of the bee swarm in finding better hives. This algorithm is similar to Particle Swarm Optimization (PSO) but it works differently. The algorithm is very powerful and has better results compared to other common metaheuristic algorithms. This paper aims at improving the performance of FDO, thus, the chaotic theory is used inside FDO to propose Chaotic FDO (CFDO). Ten chaotic maps are used in the CFDO to consider which of them are performing well to avoid local optima and finding global optima. New technic is used to conduct population in specific limitation since FDO technic has a problem to amend population. The proposed CFDO is evaluated by using 10 benchmark functions from CEC2019. Finally, the results show that the ability of CFDO is improved. Singer map has a great impact on improving CFDO while the Tent map is the worst. Results show that CFDO is superior to GA, FDO, and CSO. Both CEC2013 and CEC2005 are used to evaluate CFDO. Finally, the proposed CFDO is applied to classical engineering problems, such as pressure vessel design and the result shows that CFDO can handle the problem better than WOA, GWO, FDO, and CGWO. Besides, CFDO is applied to solve the task assignment problem and then compared to the original FDO. The results prove that CFDO has better capability to solve the problem.
Determinants of consistent condom use among Filipino women: Results from the 2017 Philippine National Demographic and Health Survey
Authors: Pepito Veincent Christian , Amit Arianna Maever L , Tang Clinton S , Molen Ryan Gabriel C , Co Luis Miguel B , Aliazas Neil Andrew Kiamco , Reyes Sarah J De Los , Tanchanco Lourdes Bernadette Sumpaico 
Published: June 2021

Abstract
Background: Consistent condom use had been perennially low in the Philippines, despite being a tested and proven public health intervention to promote unwanted pregnancy and sexually transmitted infections. Considering the high fertility rate, teenage pregnancy rate, and the rapidly increasing incidence of HIV in the country, we identify the determinants of consistent condom use in the country. Methods: We used data from the individual recode of the 2017 Philippine National Demographic and Health Survey. We used logistic regression for survey data to identify factors associated with consistent condom use. Results: Out of 25,074 respondents, only 261 (1.13%) have used condoms consistently with their most recent partner. Reach of information and education campaigns on contraceptive use via different media ranged from 62% via television to 7% via SMS. After adjusting for confounders, we find that respondents who can ask their partners to use a condom during intercourse have 6.31 times higher odds (95% CI: 2.08, 19.16) of using condoms consistently as compared to respondents who are unable to do so. We also find that current non-users of any form of contraceptive method but intend to use it later have 8.94 times greater odds (95% Confidence Interval (CI): 2.41, 33.18) and those who use modern contraceptive methods have 59.67 times greater odds (95% CI: 21.14-168.47) of being a consistent condom user as compared to non-users of any contraceptive method who do not intend to use it later. Conclusions: The low percentage of those who use condoms consistently, together with the low reach of information and education campaigns highlight the need to further intensify these efforts especially in older women beyond the reach of school-based sex education interventions. Interventions to improve sexual agency, such as improving sex education, living conditions, and social support should also be implemented to increase the prevalence of consistent condom use in the Philippines.
Leonotis Nepetifolia Mediated Eco-Friendly Synthesis of Zno Nps: Photocatalytic, Antioxidant Activities and Their Applications to Nano-Composite Electrode Material for Supercapacitor
Authors: Pavithra Gajendran , kannappan Santhakumar 
Published: June 2021

Abstract
The present paper describes the green synthesis of Zinc oxide nanoparticles (ZnO NPs) from the flowers of L. nepetifolia. The synthesis of ZnO nanoparticles and examined by using Ultraviolet-visible spectroscopy, Fourier Transform-Infrared spectroscopy, X-ray Diffraction analysis, Dynamic Light Scattering analysis, Raman spectroscopy, Scanning Electron Microscopy and Energy Dispersive X-ray spectroscopy, Transmission Electron Microscopy, and Thermogravimetric Analysis. The photocatalytic studies were followed using methylene blue (MB) dye by ZnO nanoparticles by using sunlight as a source. The degradation of MB dye is found to be 90 %. Then the synthesized ZnO nanoparticles help to evaluate the antioxidant activities. The antioxidant activities of ZnO nanoparticles were exhibiting through scavenging of Nitric oxide, Hydrogen peroxide, and DPPH free radicals. Furthermore, the electrochemical analysis of reduced Graphene Oxide-Zinc oxide (rGO-ZnO) nanocomposite shows that the prepared rGO-ZnO nanocomposite has a high specific capacitance of about 667 F g-1 in comparison with the pure Zinc oxide nanoparticles (200 F g-1) and has good cycling stability of around 1000 cycles. The synthesis of multifunctional ZnO nanoparticles by using natural plant products like seeds, leaves, flowers, etc may help to explore as environment-friendly which is opposite to chemical synthesis.
High prevalence of Sarcocystis funereus sp. nov. (Apicomplexa, Sarcocystidae) in offspring of Tengmalm’s owls Aegolius funereus (Aves, Strigidae)
Authors: Máca Ondrej , Kouba Marek , Korpimäki Erkki , González-Solis David 
Published: June 2021

Abstract
Background

Birds act as intermediate or definitive hosts of cyst-forming coccidia parasites of the genus Sarcocystis Lankester, 1882. However, the spectrum of species of Sarcocystis in birds and the role of the latter in the transmission of coccidia are still incomplete for many avian species including Tengmalm’s owl Aegolius funereus (Linnaeus, 1758). During a research of Tengmalm’s owls in Finland some fledglings were found dead and subsequently parasitologically examined. Therefore, this study is focused on the morphological and molecular description of a new Sarcocystis species found in the intestine of the Tengmalm’s owl and its possible role as definitive host.
Methods

Eleven fledgling owls from the Kauhava region of west-central Finland were found dead and subsequently were submitted for necropsy, parasitologically examined through flotation-centrifugation coprological technique for the presence of oocysts/sporocysts of genus Sarcocystis by light microscopy. Wet mounts were used for the examination of muscle samples (breast, legs, heart). Polymerase chain reaction and nested-PCR were carried out by using primers for 18S rRNA, 28S rRNA, ITS1 region and cox1 genes.
Results

All eleven examined birds were parasitized by numerous sporocysts and oocysts in the intestinal mucosa scrapings (prevalence 100%). Sporulated oocysts and sporocysts measured 16.34 − 16.96 × 11.47 − 12.09 µm and 11.85 − 13.52 × 7.77 − 9.25 µm, respectively. Skeletal and heart muscles were negative for sarcocysts. The phylogenetic analysis revealed that Sarcocystis funereus sp. nov. is closely related to Sarcocystis strixi from the barred owl (Strix varia Barton, 1799) from the USA and Sarcocystis sp. isolate 5 from the European shrew (Sorex araneus Linnaeus, 1758) from the Czech Republic, but a valid species.
Conclusions

This work provided the first and the most comprehensive record on Sarcocystis from owls obtained in Finland, thus highlighting the importance of molecular data in the species identification.
Fe3O4@SiO2-L-Cu(II): An efficient and easily recoverable catalyst for reducing/degrading environmental pollutants in aqueous medium
Authors: Dadashi Jaber , Khaleghian Mohammad , Tamizdoust Babak Mir 
Published: June 2021

Abstract
In the present study, a magnetically recoverable catalyst is synthesized through immobilizing copper (II) over the Fe3O4@SiO2 nanoparticles (NPs) surface [Fe3O4@SiO2-L-Cu (II)] (L = pyridine-4-carbaldehyde thiosemicarbazide). Accordingly, synthesized catalysts were determined and characterized by energy dispersive X-ray spectrometry (EDS), Fourier transforms infrared spectroscopy (FT-IR), X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM), vibrating sample magnetometer (VSM), and thermogravimetric-differential thermal analysis (TG-DTA) procedures. Using [Fe3O4@SiO2-L-Cu (II)], 4-Nitrophenol (4-NP), Cr(VI), and organic dyes, e.g., Methylene blue (MB) and Congo Red (CR) were reduced in aqueous media. As shown by results of Catalytic performance investigations, [Fe3O4@SiO2-L-Cu (II)] performance was outstanding concerning reduction reactions in mild conditions. Remarkable attributes of this method are high efficiency, removal of a homogeneous catalyst, easy recovery from the reaction mix, and uncomplicated route. The amount of activity in this catalytic system was almost constant after several stages of recovery and reuse. The results show that the catalyst was efficient and easily recoverable in the successive run without a significant loss of catalytic efficiency.
Timing of cholecystectomy after percutaneous cholecystostomy for acute cholecystitis- A systematic review and meta-analysis
Authors: VASAVADA BHAVIN , Patel Hardik 
Published: June 2021

Abstract
Introduction: This meta-analysis aimed to evaluate outcomes of early versus late cholecystectomy after percutaneous cholecystostomy.Methods:The study was conducted according to the PRISMA statement and MOOSE guidelines. Heterogeneity was measured using Q tests and I2 statistics. The random-effects model was used.Results: Six studies including 18640 patients were included in the final analysis. There was no difference in overall complications within or after 72 hours cholecystectomy group, but mortality and biliary complications were significantly high in the less than 72 hours group (p=0.05 and 0.0002 respectively). There was no difference in mortality, overall complication, biliary tract complications in less than 1 week versus more than 1 week and less than 10 days versus more than 10 days group. Overall complications were significantly less in the less than 2 weeks group compared to the more than 2 weeks group. There was no difference in mortality and biliary tract complications between less than 2 weeks and more than 2 weeks group. Overall complication rate (risk ratio 0.67, p <0.0001), postoperative mortality (risk ratio 0.46, p=0.003), bile duct injury (risk ratio 0.62, p=0.01) was significantly less in earlier than 4-week group. Hospital stay was not significantly different between less than 4 weeks versus more than 4 weeks group. (Mean difference= -2.74, p=0.12). Ove all complication rates were significantly more in less than 8 weeks group. (Risk ratio 1.07, p=0.01).Conclusion:Early cholecystectomy preferably within 4 weeks after percutaneous cholecystostomy is preferable.
Enhancing the 3rd order nonlinear optical response of integrated micro-ring resonators with graphene oxide 2D films
Authors: Moss David 
Published: June 2021

Abstract
Layered two-dimensional (2D) graphene oxide (GO) films are integrated with micro-ring resonators (MRRs) to experimentally demonstrate enhanced nonlinear optics in the form of four-wave mixing (FWM). Both uniformly coated and patterned GO films are integrated on CMOS-compatible doped silica MRRs using a large-area, transfer-free, layer-by-layer GO coating method together with photolithography and lift-off processes, yielding precise control of the film thickness, placement, and coating length. The high Kerr nonlinearity and low loss of the GO films combined with the strong light-matter interaction within the MRRs results in a significant improvement in the FWM efficiency in the hybrid MRRs. Detailed FWM measurements are performed at different pump powers and resonant wavelengths for the uniformly coated MRRs with 1 − 5 layers of GO as well as the patterned devices with 10 − 50 layers of GO. The experimental results show good agreement with theory, achieving up to ~ 7.6-dB enhancement in the FWM conversion efficiency (CE) for an MRR uniformly coated with 1 layer of GO and ~ 10.3-dB for a patterned device with 50 layers of GO. By fitting the measured CE as a function of pump power for devices with different numbers of GO layers, we also extract the dependence of GO’s third-order nonlinearity on layer number and pump power, revealing interesting physical insights about the evolution of the layered GO films from 2D monolayers to quasi bulk-like behavior. These results confirm the high nonlinear optical performance of integrated photonic resonators incorporated with 2D layered GO films.
Root system architecture traits diversity and association with grain yield for drought adapted sorghum genotypes
Authors: Tamir Habtamu Demelash , Menamo Temesgen Matiwos , Mindaye Taye Tadesse 
Published: June 2021

Abstract
Background and aims The roots system architecture of sorghum is a major morphological trait which strongly influences the capacity to access soil moisture and forage nutrients under drought condition. This study was conducted aiming to the extent of genetic diversity among sorghum genotypes adapted the dryland; to identify promising genotypes for key root and shoot traits for breeding purposes; and to determine the contribution of root related traits of sorghum on moisture stress area. Methods The experiment was conducted using 214 genotypes in greenhouse of Horticulture and Plant science Department, Jimma University using randomized complete block design. Results Cluster analysis indicated that the genotypes grouped into eight cluster. Number of genotypes per cluster varied from 100 (46.72%) in Cluster II and 2 (0.93%) genotypes both in Cluster III and VI .The maximum genetic distance (D 2 =151.36) obtained between cluster III and VIII and minimum genetic distance (D 2 =9.38) was found between cluster V and VII also the intra-cluster distance was highest in the cluster II (D 2 =43.93) and the lowest cluster found VI (D 2 =0.93). Root angle and root length exhibited strong negative(r=- 0.24 ) and positive correlation (r= 0.32 ) with grain yield. Genotypes Acc#220249(58); Acc#220254(#59); Acc#234102(#102); Acc#235811(#118) Acc#20710(27) the most divergent on the principal axis of biplot and used direct sorghum improvement. Conclusion The information generated from this study could help to identified promising genotypes with suitable root traits for potential breeding under specific environmental conditions.
Infection with SARS-CoV-2 lineage B.1.1.7 in Three Malayan Tigers at the Virginia Zoological Park
Authors: Mitchell Patrick K. , Martins Mathias , Reilly Tara , Caserta Leonardo C. , Anderson Renee , Cronk Brittany D. , Murphy Julia , Goodrich Erin L. , Diel Diego G. 
Published: June 2021

Abstract
We report three cases of SARS-CoV-2 lineage B.1.1.7 infection in Malayan tigers at the Virginia Zoo. All three animals exhibited respiratory signs. These findings show the mutations in the B.1.1.7 lineage did not affect the susceptibility of tigers to SARS-CoV-2.
UP-Regulation of Dicer and Drosha Expression in Ovarian Cancer Tissues
Authors: razmkhah sanaz 
Published: June 2021

Abstract
BACKGROUND: miRNAs show to play fundamental roles in diverse cellular processes and associate with a variety of cancers. DROSHA and DICER are two major enzymes in the miRNA maturation process. OBJECTIVE: Dicer and Drosha genes expression investigated Ovarian Cancer and analyzed the impact of clinicopathological characteristics on their expression. METHODS: The present study has performed on 50 cancer patients (50 tumor tissue samples and 50 marginal tissue samples) overall, 100 specimens referred to Al-Zahra Hospital's surgical department in Tabriz and Motahari Hospital Urmia for identification and treatment during 2018 and 2019. After extracting total RNA and cDNA Synthesis, using the HRM-PCR method, the rate of change in the expression of DROSHA and DICER genes assessed using the 2-ΔΔCT method. Data were analyzed using Paired t-test and Kolmogorov–Smirnov test. RESULTS: Our study showed a significant difference in the rate of expression changes in DROSHA and DICER genes in tumor tissue compared to marginal tissue. This difference was associated with an increase in DROSHA and DICER gene expression.CONCLUSIONS: Up-regulated expression of miRNAs biogenesis machinery enzymes (Dicer and Drosha) during ovarian cancer can alter the miRNA expression involved in the pathogenesis of malignancy.
Bioremoval of PVP coated silver nanoparticles using Aspergillus niger: the role of exopolysaccharides
Authors: Gomaa Ola M. , Elrshim Amar , Chanda Anindya 
Published: June 2021

Abstract
The rapidly growing production and applications of Engineered Nanoparticles (ENPs) foresees a concomitant increase in the exposure of humans to their potential toxic effects through accidental release to the environment. Due to the limited and/or contaminated water resources, the need to re-use treated water has become imperative. The present work aims to study the removal of Polyvinylpyrrolidone coated silver nanoparticles (PVP-Ag-NPs) using Aspergillus niger and depict the role of exopolysaccharides in the removal process. Our results show that the majority of PVP-Ag-NPs were attached to fungal pellets. About 74% and 88% PVP-Ag-NPs were removed when incubated with A. niger pellets and exopolysaccharide-induced A. niger pellets, respectively. Ionized Ag decreased by 553 and 1290 fold under the same conditions as compared to stock PVP-Ag-NP. The increase in PVP-Ag-PVP concentrations resulted in an increase in reactive oxygen species (ROS) in 24h. The UV-Visible spectrum shows the disappearance of Ag characteristic peak and the broadness of the spectrum suggested an increase in size. Dynamic Light Scattering results showed an increase in PVP-Ag-NPs size from 28.4 nm to 115.9 nm for A. niger pellets and 160.3 nm after removal by stress-induced A. niger pellets and further increased to 650.1 nm for in vitro EPS removal. Our findings show that EPS can be used for nanoparticle removal, by increasing the net size of nanoparticles in aqueous media, this will, in turn, facilitate its filtration through conventional filtration techniques commonly used at wastewater treatment plants.