bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Genetics and Clinical Variables of Retinopathy of Prematurity: A Matched Case Control Study
Authors: Zhao Congcong , Wang Zhansheng , Lu Yuebing , Li Li , Liu Lei , Liu Jing , Sun Huiqing 
Published: November 2021

Abstract
Purpose: Retinopathy of prematurity (ROP) is a major cause of childhood blindness worldwide; it is a proliferative retinal vascular disease in preterm infants. Some infants progress to severe disease despite absence of these clinical risk factors such as low birth weight, gestational age and oxygen. Genetic factors seem to have etiologic roles in retinopathy of prematurity (ROP). This matched case-control study examined genetic factors for ROP.Methods: Confirmed 71 severe ROP cases and gestational age, birth weight and days of oxygen therapy matched controls (1:1) were enrolled from September 2015 to August 2020. Exome sequencing was performed and accomplished by next-generation sequencing. Gene mutations on heritable retinal vascular diseases related to Norrin signaling pathway were analyzed. Results: Seven heterozygous variants in exon 2 and the 3′ UTR were identified in the FZD4 gene in 28 patients with ROP. Variants c.*3770A > T and c.*2971T > C were found to be significantly associated with ROP (P = 0.033 and P = 0.017 respectively). Screening of LPR5 revealed five heterozygous variants. The rate of variant c.575 + 6T > C was much higher in infants with ROP (P = 0.009). One variant in exon 3 (c.67-9delT) was found in the TSPAN12 gene in one infant with ROP, whereas another variant of TSPAN12, c.*1139A > T, was identified in both groups. Two variants of ZNF408, c.581_592del AGTGGTGACAGA and c.1007C > T, were found in infants with ROP, with the rate of change of the former being much higher in infants with ROP (P < 0.001 and P = 0.033 respectively). Conclusions: This study revealed genetic factors in Norrin signaling may contributed to the development and severity of ROP, which warrants further detailed investigation worldwide.
Identification of Persons Who Are Responsive To Wood Smoke Particle-Induced Airway Inflammation With Assessment of The Effect of GSTM1, Asthma Status And Sex On This Response.
Authors: Peden David B. , Zhou Laura , Burbank Allison J. , Almond Martha , Hernandez Michelle L. , Mills Katherine H , Noah Terry L. , Wells Heather , Zhou Haibo , Alexis Neil E. 
Published: November 2021

Abstract
Background: We are currently screening human volunteers to determine their sputum polymorphonuclear neutrophil (PMN) response 6 and 24 hours following initiation of exposure to wood smoke particles (WSP). Inflammatory responders (>10% increase in %PMN) are identified for their subsequent participation in mitigation studies against WSP-induced airways inflammation. In this report we compared responder status (N=52) at both 6 and 24hr time points to refine/expand its classification, assessed the impact of the GSTM1 genotype, asthma status and sex on responder status, and explored whether sputum soluble phase markers of inflammation correlate with PMN responsiveness to WSP. Results: In the entire cohort, we found a significant, but very small, decrease in FVC and systolic blood pressure immediately following WSP exposure and sputum %PMNs were significantly increased at 24 hours post exposure, the latter finding was also significantly correlated with sputum IL-1b, IL-6, IL-8, and PMN/mg; a similar response was not found at the 6 hour %PMN response. Blood endpoints in the entire cohort showed a significant increase in %PMN and PMN/mg at 6 but not 24 hours. Six-hour responders tended to be 24-hour responders and vice versa, but 24-hour responders also had significantly increased IL-1b, IL-6, IL-8 at 24 hours post WSP exposure. The GSTM1 null genotype significantly (p<0.05) enhanced the %PMN response at 6 hours in the entire cohort, by 24% in the 24-hour responders and not at all in the 6 hours responders. Asthma status enhanced the 24 hour %PMN response in the entire cohort and in the 6- and 24-hour responders. Sex had no effect on %PMN response. Conclusions: The 24 hour time point was more informative than the 6 hour time point in optimally defining airway inflammatory responsiveness to WSP exposure. GSTM1 and asthma status are significant effect modifiers of this response. These study design and subject parameters should be considered before enrolling volunteers for proof-of-concept WSP mitigation studies.
Neonatal Multi-System Inflammatory Syndrome Associated With Covid-19 Exposure in Two Cases From Iran
Authors: Saeedi Maryam , Mirnia Kayvan , Sangsari Razieh , Makan Zeinab Jannat , Ziaee Vahid 
Published: November 2021

Abstract
Introduction:Immune dysregulation following exposure to Covid-19 results in MIS-N (Multi-system Inflammatory Syndrome in Neonates). MIS-N affects various systems in the body and is diagnosed with a positive history of PCR test, positive serologic test, and a history of contact with those vectors of COVID-19 infection. This case series aimed to differentiate from possible misdiagnosis about MIS-N.Case Presentation:Both cases are term neonates with positive serology of COVID -19 and the 2nd case with a mother's positive history of Covid-19 PCR at 30 weeks of pregnancy. The first case was admitted with diarrhea, dehydration, fever for three days, and rash on the 3rd day of hospitalization. We admitted the 2nd case on the 22nd day of birth with a cough, rashes on the head, palms, and soles for two days. Both cases responded to corticosteroid treatment that confirmed MIS-N. Finally, we discharged them with a stable and normal condition in follow-ups.Conclusions:In inflammatory syndromes, especially in delayed phases of COVID cytokine storms, the mortality and morbidity caused by infections diminish with proper interventions and inhibited cytokine cascade inflammations.
Knowledge, Attitude, and Prevention Practice on Dengue and Dengue Seroprevalence in a Dengue Hotspot in Sabah, East Malaysia
Authors: Guad Rhanye Mac , Sim Maw Shin , Wu Yuan Seng , Aung Yin Nwe , Low Wah Yun , Sekaran Shamala Devi , Azizan Nornazirah 
Published: November 2021

Abstract
Background: Dengue, a major global public health concern, is growing more prevalent. Extensive measures have been undertaken to prevent and control dengue transmission in Malaysia including understanding and modification of human behaviour. This study was designed to assist in planning an effective health intervention for dengue by measuring residents’ knowledge, attitudes, and practice (KAP) in hotspot areas of Sabah, East Malaysia. Methods: A community-based and cross-sectional study was conducted at dengue hotspot area in Sabah involving 463 residents (aged 18 years and above). Information on the socio-demographic characteristics of the residents and their KAP towards dengue was collected using a structured pre-validated questionnaire developed by the researchers. In addition, residents were invited to participate in a dengue seroprevalence study using dengue Immunoglobulin M (IgM) and Immunoglobulin G (IgG) ELISA kit. Results: Most of the residents correctly identified common signs and symptoms of dengue such as fever (84.4%), chills (70.7%), and nausea or vomiting (56.0%). Nearly half of the residents (51.3%) had good knowledge, 66.0% had a positive attitude, and 63.7% had a positive practice for dengue prevention and control, respectively. The dengue seroprevalence study included 200 of the 463 residents; IgG and IgM seropositivity was 74.1% (n = 63) and 7.1% (n = 6) respectively. Based on logistic regression analysis, local and migrants differs considerably in terms of their knowledge and attitude toward dengue prevention and control, while gender and level of education were predictive of practice level (p<0.05) among all sociodemographic variables examined. Knowledge was correlated with attitude (p<0.0001) and practice (p< 0.0014). Discussion: To combat dengue epidemics in the hotspot area of Sabah, a proactive and behavioural changes among residents, are required. To effectively prevent and manage dengue transmission, a collaborative effort of government sectors, private sectors, housing residential committees, and community residents is essential.
The Effect of Short-chain Fatty Acid Supplementation on Acute Colonic Inflammation in Rats Fed With Western Diet: Role of the Peroxisome Proliferator-activated Receptor-gamma
Authors: Sevim Mustafa , Çantalı-Öztürk Çiğdem , Mergen Ali , Güllü Eda Ceren , Ayvaz Elif Yaren , Ölüç Merve , Temel Merve , Şirvancı Serap , Yüksel Meral , Çevik Özge , Yeğen Berrak Çağlayan , Yıldırım Alper 
Published: November 2021

Abstract
Increased incidence of inflammatory bowel disease (IBD) is associated with the consumption of westernized diet (WD). In male Wistar albino rats induced with colitis by oral intake of dextran-sulfate sodium (DSS) for 10 days, we investigated how colitis-induced oxidative damage is affected by WD consumption started 3 weeks before DSS intake. Secondly, the putative protective effects of short-chain fatty acids (SCFAs) were evaluated in both WD-fed and normal diet (ND)-fed rats. WD consumption prior to colitis induction alleviated oxidative damage and suppressed inflammatory response of the colon via the modulation of peroxisome proliferator-activated receptor (PPAR)-γ activity, suggesting a preconditioning impact of the WD. Moreover, addition of SCFA to WD abolished the elevations in lipid profile, while SCFA added to either ND or WD during the 10-day development of colonic inflammation depressed the pro-inflammatory TNF-α and IL-6 expressions and upregulated IL-10, but the reduction in oxidative damage due to WD was not further changed. The results demonstrated that acute colonic inflammation is alleviated when it is preceded by the consumption of WD, and SCFAs improved WD-induced disruption in lipid profile, implicating that SCFAs would be advantageous in IBD patients comorbid with metabolic syndrome.
Glucagon-Like Peptide-1 Receptor Agonist, Liraglutide, Attenuated Diabetic Retinal Edema in Spontaneously Diabetic Torii Fatty Rats
Authors: Inoue Kazuho , Yamada Shohei , Hoshino Seiko , Watanabe Minoru , Kimura Kenjiro , Kamijo-Ikemori Atsuko 
Published: November 2021

Abstract
Background: This study aims to investigate the effect of the glucagon-like peptide-1 (GLP-1) receptor agonist (GLP-1RA) liraglutide on retinal edema as compared with insulin and hydralazine using an animal model of type 2 diabetes with obesity, hypertension, and hyperlipidemia.Methods: Male spontaneously diabetic Torii (SDT) fatty rats at 8 weeks of age were randomly assigned to three groups: the liraglutide group (SDT-lira, n = 6) received a subcutaneous injection of liraglutide from the age of 8 to 16 weeks, the SDT-ins-hyd group (n = 6) was provided both insulin against hyperglycemia and hydralazine against hypertension to match levels of both blood glucose and blood pressure to those of the liraglutide group, and the control group of SDT fatty rats (SDT-vehicle, n = 7) and a nondiabetic control group of Sprague–Dawley rats (SD, n = 7) were injected with vehicle only. Both eyeballs of all groups were collected at the age of 16 weeks.Results: Retinal thickness, which was found in the SDT-vehicle group, was significantly prevented to similar levels in both the SDT-lira and SDT-ins-hyd groups. Immunohistological analysis revealed that GLP-1 receptor was not expressed in the retina of all rats. The ocular protein expression of monocyte chemoattractant protein-1, which causes a proinflammatory situation, was significantly upregulated in all SDT fatty rats as compared to SD rats, but the expression levels were similar between all SDT fatty rats. With regard to neovascularization in the eyes, there were no significant differences in protein expressions of vascular endothelial growth factor, CD31, or endothelial nitric oxide synthase in all rats.Conclusions: The present study indicates that liraglutide prevents retinal thickening, dependent on blood glucose and blood pressure levels in SDT fatty rats without ocular neovascularization. However, the effects did not improve the ocular proinflammatory state.
Designing of Fabric Reinforced Polymer in the Assessment of Sorption Capacity of Mussel Shell Powder for Divalent Ion: GIS Mapping and Statistical Studies
Authors: Charulatha S. , Thangamani K.S. , Andal N. Muthulaksmi 
Published: November 2021

Abstract
This paper discusses the efficiency of acid modified mollusc shell powder in adsorbing Zn(II) ions from synthetic wastewater and actual wastewater samples discharged from electroplating industries. The chemically treated adsorbent was characterized in terms of structure and surface properties. The parameters such as, size of the sorbent particles, dosage, initial Zn(II) concentration, temperature, contact time pH and temperature were varied for optimization using batch studies. The adsorbate-adsorbent system was studied by Langmuir, Freundlich, Tempkin and DKR isotherms. The adsorption experiments were conducted in a column packed with the acid modified mollusc shell powder. Further, real wastewater samples having Zn(II) concentration higher than the permissible levels were collected from the industrial belt in Coimbatore, to assess the potential of the bio adsorbent. A fiber reinforced polymer fabricated with the adsorbent material installed at the effluent discharge plants achieved 100% Zn(II) removal from electroplating wastewaters. Statistical analysis performed using SPSS software showed significant correlation among the input parameters and the analytical results post-adsorption.
Effect of Paratuberculosis Vaccination on the in Vitro Release of Neutrophil Extracellular Traps in a Sheep Model
Authors: Arteche-Villasol Noive , Gutiérrez-Expósito Daniel , Criado Miguel , Benavides Julio , Pérez Valentín 
Published: November 2021

Abstract
Background
Vaccination of domestic ruminants against paratuberculosis has shown beneficial results in the control of the disease, even though protection is incomplete. This is partly due to the unawareness about the mechanisms taking part during protective immune response. In this sense, despite of the increased number of evidences that point out the participation of neutrophils in the homologous and heterologous protection, its key role is still unclear. Therefore, we evaluate the effect of paratuberculosis vaccination on the release of neutrophil extracellular traps (NETs), through its quantification and visualization after their in vitro infection with Mycobacterium avium subsp. paratuberculosis (Map) and non-related pathogens in a sheep model.
Methods
Ovine neutrophils were obtained from non-vaccinated (n = 5) and vaccinated sheep (n = 5) and infected in vitro with Map, Staphylococcus aureus (SA) and Escherichia coli (EC). After 4 hours, NETs release was quantified and visualized by fluorimetry and immunofluorescence microscopy, respectively.
Results
NETs components were confirmed by co-localization of extracellular DNA with neutrophil elastase and myeloperoxidase visualized by immunofluorescence. Infection of ovine neutrophils with Map (P > 0.05), SA (P < 0.001) and EC (P < 0.01) induced the formation of NETs before vaccination as well as after 15- and 30-days post-vaccination. However, neither vaccination nor time post-vaccination did modify the generation of NETs.
Conclusions
These results show that the release of NETs did not play a fundamental role in the early homologous and heterologous protective immune response after paratuberculosis vaccination, although, other antimicrobial mechanisms not evaluated in this study may participate in the protective immune response.
Effect of Zinc Coating on Delay Nugget Formation in Dissimilar DP600 - AISI304 Welded Joints Obtained by the Resistance Spot Welding Process
Authors: Parte Mercedes Pérez de la , Hernández Alejandro Espinel , Orozco Mario César Sánchez , Roca Angel Sánchez , Macias Emilio Jiménez , Fernández Julio Blanco , Fals Hipólito Domingo Carvajal 
Published: November 2021

Abstract
This paper researches the effect of zinc coating of galvanized DP600 steel on the dynamic resistance and the delayed nugget formation of dissimilar DP600 - AISI304 welded joints, obtained with resistance spot welding process (RSW). The RSW evaluations consisted of determining, from the dynamic resistance curves, the time involved in the different stages of the process, particularly the beginning of nugget formation. The experimental results showed that, from the dynamic resistance curves, it is possible to identify 8 distinct stages during the welding of galvanized DP600 steel and AISI304 stainless steel. In the case of the welding of uncoated DP600 steel with AISI304, only 6 stages are identified (except for stages 2 and 3), which are directly related to the heating, softening and melting of the galvanic coating. The energy used in stages 2 and 3, causes a delay in the beginning of nugget formation for welded joints obtained with galvanized DP600 steel compared to uncoated DP600 - AISI304 welded joints, reaching values between 37.28 ms and 52.29 ms for the welding conditions analyzed. Monitoring the time duration of stages 2 and 3, as defined from the analysis of the dynamic resistance curves, could be used as a tool to predict the beginning of nugget formation in the welding of galvanized steels, to avoid undesirable phenomena such as expulsion and to guarantee the quality of the welded joints.
Evaluation of Iodine Supplementation in Women With Pregnancy Associated With Gestational Diabetes. IODIAB Study
Authors: DARDARI DURED , Laborne Francois-Xavier , Tourte Caroline , Henry Elodie , Penfornis Alfred 
Published: November 2021

Abstract
Iodine supplementation is indicated by the French High Authority for Health (HAS) and by the World Health Organization (WHO) during pregnancy. We want to know if this supplementation is prescribed in a manner consistent with the HAS and WHO recommenations in women diagnosed with gestational diabetes mellitus.Results: Recruitment for the study will start August 2021 and will be ended in October 2021, with the final study results scheduled for December 2021Conclusion The objective of the Iododiab study is to assess whether practitioners caring for women with gestational diabetes mellitus prescribe iodine supplementation according to the recommendations of the French High Authority for Health and by the World Health Organization.
The evolving role of microRNA subtypes 21, 155 and 10b as oncogenes and biomarkers in breast cancer in a decade: A systematic review article
Authors: Elnour Mubarak , Eltayeb Nagham , Esam Arwa , Faisal Shiraz , Elhassan Nuha M. 
Published: November 2021

Abstract
Breast cancer is a malignant disease with a high incidence worldwide in women-and to less extent in men-, thus remains a health burden. miRNA plays a key role in the development and prognosis of breast cancer; as a single miRNA has the ability to regulate multiple gene targets and adversely alter their expression. In this review, we attempt to systematically analyze the rapidly accumulating body of research regarding the role of miRNAs subtypes particularly as oncogenes and biomarkers in breast cancer, and their clinical implications. A total of 11 pertinent articles in the past 10 years were extracted from high-rank reviews, analyzed, and summarized. Amongst many (eight miRNA subtypes), three subtypes (miR21, 155, and 10b) were selected in order to evaluate the validity of their oncogenicity as well as their diagnostic value as biomarkers.
The Viral Respiratory Diseases of Sorok Island at Pandemic
Authors: Lee Jong-Hoon , Lee Chul Joong , Lee So Jeong , Choi Su-Hee , Oh Sang-Suk , Park Jungwuk , Sergi Consolato , Coleman Michael D. 
Published: November 2021

Abstract
Background: Dapsone is helpful in the molecular regulation of Nod-like receptor family pyrin domain-containing 3 (NLRP3).Objective: To study the targeting of NLRP3 itself or up-/downstream factors of the NLRP3 inflammasome by Dapsone should be responsible for its observed preventive treatment effects, functioning as a competitor against Pandemic viral inflammasome.Methods: We compared Hansen's disease (HD) patients with viral respiratory diseases (VRD) after prescribing Dapsone to standard treatment from 2005 to 2020. Results: The 3022 VRD participants who received the dapsone intervention (M = 201, SD = 34) compared to the 3961 VRD participants in the control group (M = 264, SD = 84) demonstrated significantly better peak flow scores, t(28) = -2.7, p = .01. It demonstrated significantly more prevalences of VRD in the DDS unprescribed group. Conclusion: This study is theoretical clinical data to warrant a pilot study with Dapsone for deteriorating leprosy patients at Pandemic.
“HIGH sensitivity Troponins In Patients With Elevated Pro BNP and Acute Heart Failure”. (HIGH-TRIP Trial)
Authors: AlHejily Wesam A 
Published: November 2021

Abstract
Background:In patients presented to emergency rooms NT-Pro BNP essay is overly sensitive test to rule out heart failure but less specific in predicting outcomes in follow-ups, in this study we ought to find the added value of HS-Troponin I, in patients presented acutely with heart failure and its impact on mortality when Pro BNP is highly elevated.Methods:Prospective cohort study, inclusion criteria were age above 18 and clearly positive NT Pro BNP > 1000 pg/ml, with 12 months follow up period, primary end point was mortality from heart failure, secondary endpoint was need for rehospitalization.Results:95 patients were enrolled, divided into overt and non-overt pulmonary edema groups. Mean (NT-Pro BNP) was 6184 and 5927 pg/ml and mean (Hs-c Trop I) were 19.27 and 0.17ng/ml respectively, Mean Ejection fraction was 48+/-7 and 47+/-7 for each group sequentially. Mortality rate was 4 (13%) in the higher Hs-c Troponin I group, and 1 (1.6%) in the low troponin level group p=.03, odd ratio was 8.5, 95% CI (0.9-80). Need for re-hospitalization was present in 12 (38%) Vs 7 (8%) patients, p=.0081, odd ratio 4.8, 95% CI (1.7-14.2). In COX proportional hazard analysis, only Hs-cTN was a significant predictor of poor outcome in this high-risk cohort with p=0.0001.Conclusion: Adding (Hs-cTroponin I) assay to the panel of laboratory testing, in patients presented to ER with acute heart failure and with high Pro-BNP>1000, may further predicts mortality and rehospitalization rate.
A review of one-phase Hele-Shaw flows and a level-set method for nonstandard configurations
Authors: Morrow Liam C. , Moroney Timothy J. , Dallaston Michael C. , McCue Scott W. 
Published: November 2021

The classical model for studying one-phase Hele-Shaw flows is based on a highly nonlinear moving boundary problem with the fluid velocity related to pressure gradients via a Darcy-type law. In a standard configuration with the Hele-Shaw cell made up of two flat stationary plates, the pressure is harmonic. Therefore, conformal mapping techniques and boundary integral methods can be readily applied to study the key interfacial dynamics, including the Saffman–Taylor instability and viscous fingering patterns. As well as providing a brief review of these key issues, we present a flexible numerical scheme for studying both the standard and nonstandard Hele-Shaw flows. Our method consists of using a modified finite-difference stencil in conjunction with the level-set method to solve the governing equation for pressure on complicated domains and track the location of the moving boundary. Simulations show that our method is capable of reproducing the distinctive morphological features of the Saffman–Taylor instability on a uniform computational grid. By making straightforward adjustments, we show how our scheme can easily be adapted to solve for a wide variety of nonstandard configurations, including cases where the gap between the plates is linearly tapered, the plates are separated in time, and the entire Hele-Shaw cell is rotated at a given angular velocity.

doi:10.1017/S144618112100033X