bluuebunny/crossref_metadata_2025_split huggingface dataset:
Postoperative Evaluation of Knees With Posterior Cruciate Ligament Injury Using Patient-Oriented Evaluation Method: Comparison of Single-Bundle and Anatomic Double-Bundle Techniques
Authors: Ochiai Satoshi , Hagino Tetsuo , Senga Shinya , Furuya Naoto , Taniguchi Naofumi , Ando Takashi , Haro Hirotaka 
Published: October 2021

Abstract
Introduction Using the patient-based QOL evaluation scale SF-36 and conventional assessment methods, we evaluated the postoperative outcome of patients with posterior cruciate ligament (PCL) injury who underwent single-bundle or double-bundle reconstruction, and compared the two reconstruction techniques. Methods 37 male patients with isolated PCL injury who underwent reconstruction were randomized to receive single-bundle reconstruction (group S: n=20) or double-bundle reconstruction (group D: n=17). Before surgery and 6 and 24 months after surgery, patients were evaluated by SF-36 scores, Lysholm score, visual analog scale (VAS), posterior tibial displacement rate, and knee range of motion (ROM). Results For SF-36 evaluation at 6 months post-surgery, the scores of all the subscales improved to above the national standard values in group D, whereas none of the subscale scores reached the national standard values in group S, and three subscale scores were inferior in group S compared to group D. At 24 months post-surgery, improvement of all subscale scores to above the national standard values was achieved in both groups. Lysholm score, VAS score, and posterior tibial displacement rate improved after surgery in both groups, but no significant intergroup differences were observed in all evaluation methods. For knee ROM, residual limitation of flexion was significantly more frequent in group S than in group D at 6 and 24 months post-surgery.Conclusion Arthroscopy-assisted single-bundle PCL reconstruction technique is considered to be a safe procedure with low invasiveness, but despite its widespread use, surgical result is not consistently good. This was attributed to the low reproducibility of the unique course and anatomy of PCL, and the anatomic double-bundle reconstruction technique was proposed aiming to improve treatment outcome. According to the present results, double-bundle reconstruction tended to achieve better restoration at an early stage compared to single-bundle reconstruction, with fewer patients having residual limitation of knee flexion after surgery. Trial registration number of our hospital’s IRB: 27-8.Registered 14 September 2015, retrospectively registered.
Fertility Preservation Counselling for Women With Endometriosis: A European Online Survey
Authors: Sänger Nicole , Menabrito Marco , Sardo Atillio Di Spiezo Di Spiezo , Estadella Josep , Verguts Jasper 
Published: October 2021

Abstract
Background: Endometriosis is a common cause for infertility. Decreased ovarian reserve due to pathology or surgical management can reduce the chances of natural pregnancy and limit the effectiveness of controlled ovarian stimulation during fertility treatment. Cryopreservation of oocytes or ovarian cortex prior to surgery or before loss of follicular capital are strategies to preserve fecundity. Methods: An online survey was sent to reproductive specialists and gynaecological surgeons representing major centers of reproductive medicine in Europe to investigate current fertility preservation practices for endometriosis patients. Results: Of 58 responses, 45 (77.6%) in 11/13 countries reported the existence of endometriosis management guidelines, of which 37/45 (82.2%) included treatment recommendations for infertile patients. Most centers (51.7%) reserved fertility counselling for severe endometriosis (large endometriomas with or without deep endometriosis) while 15.5% of centers did not offer fertility preservation for endometriosis. Conclusions: To address non-uniformity in available guidelines and the diversity in fertility preservation practices, we propose an algorithm for managing patients with severe endometriosis most likely to be impacted by reduced ovarian reserve. Improved awareness about the possibilities of fertility preservation and clear communication between gynaecological surgeons and reproductive medicine specialists is mandatory to address the unmet clinical need of preventing infertility in women with endometriosis.
Treatment Outcome, Cognitive Function and Psychopathology in Methamphetamine Users Compared to Other Substance Users
Authors: Kamp Felicia , Behle Nina , Proebstl Lisa , Hager Laura , Riebschläger Marlies , Schacht-Jablonowsky Maik , Hamdorf Willem , Neumann Steffi , Krause Daniela , Manz Kirsi , Franke Andreas G. , Koller Gabi , Soyka Michael 
Published: October 2021

Abstract
Background: There is an increasing demand of treatment options for methamphetamine users. The present study evaluates differences between methamphetamine users and users of other substances with respect to cognitive function and psychopathology and possible correlates of treatment outcome. Method: 110 subjects were recruited for an observational longitudinal study from a German inpatient addiction treatment center: 55 patients with methamphetamine dependence and 55 patients with dependence of other substances (OS group). Groups were examined at beginning (baseline) and end of treatment (after six months) with regard to treatment retention, craving, cognitive functioning, psychosocial resources, personality traits, and psychiatric symptoms. Results: A total drop-out rate of 40% was observed without significant differences. At baseline, Methamphetamine-group subjects had significantly lower intelligence quotient, less years of education, poorer working speed and lower working accuracy and cannabinoid and cocaine use compared to OS-group. Methamphetamine-group subjects showed a significantly lower score of conscientiousness, psychiatric symptoms than subjects from the OS-group. Both groups showed a reduction of craving and depressive symptoms and an improvement of working speed and working accuracy after treatment. Conclusions: There are differences between methamphetamine users and users of other drugs, but not with regard to the effectiveness of treatment in this inpatient setting.
Smart Gold-Polymer Nanostructures As Promising Drug Nanocarriers For Dual-Imaging Modality And Synergistic Chemo-Photothermal Therapy in Breast Cancer Cells
Authors: Azizi Mehdi , Pakravan Asrin , Valizadeh Hadi , Rahbarghazi Reza , Dianat-Moghadam Hassan , Bani Farhad , Kahroba Houman , Salehi Roya , Mehrmohammadi Mohammad 
Published: October 2021

Abstract
To improve the efficacy of cancer therapeutics, highly sensitive imaging with precise accuracy is mandatory for timely diagnosis and selection of strategic approaches. Despite recent advances in technologies associated with tumor imaging, the application of conventional single-mode imaging is the subject of debate. Herein, two types of pH-responsive gold-polymer nanostructures, GNSts and GNRs, containing CD and MTX, GNSts-MTX@CD-Pol and GNRs-MTX@CD-Pol were designed. Dual-imaging modalities (fluorescence and CT imaging) and synergistic chemo-photothermal therapy were examined in human breast cancer MDA-MB 231 cells. MTT assay showed NIR irradiation of cells pre-treated with synthesized nanoparticles promoted tumoricidal synergy via the reduction of survival rate after 48 hours in comparison with the control group (p<0.05), indicating a high absorption coefficient in the NIR area and efficient heat production rate. Flow cytometry, real-time PCR and western blot, analyses indicated an apoptotic cell death induced by the up-regulation of Caspases 3, 6, 7, 8, and 9, Bax, and Annexin-V, confirming the activation of intrinsic and extrinsic apoptosis pathways inside the host cells. The elevation of p27 and p53 in line with the increase of cell percent at the subG1 phase showed apoptotic changes and inhibition of dynamic cell growth compared to the non-treated cells (p<0.05). Evident fluorescence intensity at lower pH values (6.3) showed pH-dependent activity of nanoparticles internalized by surface folate receptor. Of note, we showed strong capability for CT imaging in cells incubated with GNSts-MTX@CD-Pol and GNRs-MTX@CD-Pol. Taken together, all data show that gold-polymer nanostructures have considerable capability in theranostic applications like simultaneous diagnostic imaging and therapy.
Effects of a mobile health intervention on activities of stress self-management for workers: A randomized controlled trial
Authors: Lee Young Joo 
Published: October 2021

Abstract
Background: Mobile health interventions are being widely tried because of their attractive advantages. However, there is not enough evidence for its effectiveness. This study aimed to evaluate the effects of mobile app-based stress management intervention (mSMI) on highly stressed workers. Methods: A sample of 82 white-collar workers with elevated symptoms of perceived stress (Perceived Stress Scale-10≥22) were randomly assigned to the intervention or control group. The mSMI consisted of three modules: self-management including a work diary, counseling based on cognitive behavioral therapy and interventions focused on music, meditation, relaxation and image healings. Self-report data were collected the baseline and post-intervention. Study outcomes were perceived stress, anxiety, depression, and work engagement. Data were assessed using analysis of covariance with covariates. Results: There was significantly reduced perceived stress from baseline to 6 weeks in mSMI (t=5.788, p<.001) and control group (t=3.184, p=.003). After adjusting for covariates, the between-group difference in the perceived stress was significantly different (F=4.051, p=.048); however, the effect size was small. There was no significant intervention effect on anxiety, depression, and work engagement. The process evaluation indicated that most participants (85.3%) were satisfied with the intervention and their mental health benefited. Conclusion: This study found that mobile health intervention facilitated stress management for highly stressed workers. Further studies should address job-related outcomes and mental health symptoms in workers by applying the latest information technology and addressing the limitations of mobile interventions. Trial Registration: Not applicable.
WITHDRAWN: Optimization and Scale-up Methodology in Preparing Microsponge Loaded with 5-Fluorouracil (5-FU).
Authors: Halder Shahjaman , Poddar Sourav , Khanam Jasmina 
Published: October 2021

Abstract
The present investigation aims at developing models by response surface methodology (FCCCD) followed by the scale-up method in preparing control release microsponge particles loaded with 5- fluorouracil, a drug used to treat actinic keratosis and colon cancer, and producing a new Dermal Delivery System. The polymer-based (ethyl cellulose and eudragit RS 100) microsponge particles were prepared by the w/o/w double emulsification method. The optimized product was formed with the combination of independent variables levels: polymer (600 mg), stirring speed (1198 rpm) and surfactant (2% w/v), yielding responses as yield (~63.6257%), the average size of particles (~151.563 µm), entrapment efficiency (~75.319 %) and drug release in 8hr (~75.75%), with desirability value of 0.737. The products showed similar responses as obtained in scale-up work. FT-IR, DSC and SEM studies confirmed the drug's compatibility with polymers and porous morphology. Finally, gel embedded optimised product showed shear-thinning rheological property, ideal for drug release from the thixotropic gel.
Rostral Anterior Cingulate Activations Inversely Relate To Reward Payoff Maximation &amp; Predict Depressed Mood
Authors: Balasubramani Pragathi Priyadharsini , Diaz-Delgado Juan , Grennan Gillian , Alim Fahad , Zafar-Khan Mariam , Maric Vojislav , Ramanathan Dhakshin , Mishra Jyoti 
Published: October 2021

Abstract
Choice selection strategies and decision making are typically investigated using multiple-choice gambling paradigms that require participants to maximize reward payoff. However, research shows that performance in such paradigms suffers from individual biases towards the frequency of gains to choose smaller local gains over larger longer term gain, also referred to as melioration. Here, we developed a simple two-choice reward task, implemented in 186 healthy human adult subjects across the adult lifespan to understand the behavioral, computational, and neural bases of payoff maximization versus melioration. The observed reward choice behavior on this task was best explained by a reinforcement learning model of differential future reward prediction. Simultaneously recorded and source-localized electroencephalography (EEG) showed that diminished theta-band activations in the right rostral anterior cingulate cortex (rACC) correspond to greater reward payoff maximization, specifically during the presentation of cumulative reward information at the end of each task trial. Notably, these activations (greater rACC theta) predicted self-reported depressed mood symptoms, thereby showcasing a reward processing marker of potential clinical utility.
Character of Microbial Engraftment Following the Fecal Microbiota Transplantation in Patients with Inflammatory Bowel Diseases
Authors: Olekhnovich Evgenii I. , Kalachnyuk Tatyana N. , Fedorov Dmitry E. , Zhgun Elena S. , Klimina Ksenia M. , Veselovsky Vladimir A. , Boldyreva Daria I. , Matorin Roman I. , Lyamina Svetlana V. , Andreev Dmitriy N. , Maev Igor V. , Pavlenko Alexander V. , Govorun Vadim M. , Ilina Elena N. 
Published: October 2021

Abstract
Fecal microbiota transplantation (FMT) targeting gut microbiota has recently been applied in treatment of Crohn’s disease (CD) and ulcerative colitis (UC). However, only a fraction of patients respond positively to this therapy. In this regard, it is gaining popularity to study the combinations of approved medications and FMT to increase the effectiveness of therapy. Here we present a pilot longitude project for the study of FMT application combined with the anti-inflammatory therapy in CD (n = 8) and UC (n = 6) patients. Using the shotgun sequencing of stool samples (n = 56) we evaluated the temporal changes in the taxonomic and functional structure of the patients’ metagenomes and donor-derived microbes engraftment due to the FMT procedure. According to our analysis, the gut microbiota was changed both in CD and UC patients whereas reshaping nature in experimental groups was differ. Only in the UC group the shift of gut microbiota composition towards donor and the donor-derived microbes engraftment were more extensive in comparison with the CD group. Moreover, we observed that beneficial and prevailing in human population microbes such as Faecalibacterium prausnitzii, Bifidobacterium adolescentis, Eubacterium rectale, and others are the best colonizers. The difference in the microbial engraftment can be explained by differences in the pathogenesis of CD and UC. On the other hand, mucosal and intestinal lumen conditions in the UC group may have been better corrected due to the proposed treatment which allowed the donor microbes to colonize these patients more successfully. In addition, the evolutionary formed ecological relationship between the gut microbiota and the host can be linked with the FMT therapeutic effect.
Intraempreendedorismo no setor público por meio de ações formativas e educativas das escolas de governo brasileiras
Authors: Neto Alcielis De Paula , Emmendoerfer Magnus Luiz , Corrêa Stela Cristina Hott 
Published: October 2021

Melhorias interorganizacionais das instituições públicas passam pela ressignificação do seu capital humano, inclusive no que tange às competências empreendedoras. Nesta perspectiva, as Escolas de Governo (EGs) podem contribuir com a formação e capacitação de funcionários públicos. Assim, o objetivo aqui proposto é compreender as evidências de diretrizes educativas e formativas em prol do intraempreendedorismo no setor público por intermédio das EGs. Para tanto, foi realizado um estudo de casos múltiplos com três Escolas de Governo brasileiras dos três níveis federativos (federal, estadual e municipal). As entrevistas em profundidade foram submetidas à análise de conteúdo apoiadas no software IRAMUTEQ. Os resultados apontam a projeção da inovação como um pseudoempreendedorismo e a insinuação de diretrizes para um possível processo de educação para o empreendedorismo de servidores públicos, o que os tornaria potenciais intraempreendedores. Dentre essas diretrizes, destacam-se a análise do contexto, o perfil do aluno, os métodos de ensino e aprendizagem, aspectos jurídicos-legais, a adequação da infraestrutura e o papel dos facilitadores (mentores). Conclui-se que as EGs analisadas contribuem com a criação do valor público e valorização da imagem das organizações públicas por meio da ação intraempreendedora de seus servidores.
Hydrological impacts of climate and land-use change on flow regime variations in upper Indus basin
Authors: Haleem Kashif , Khan Afed Ullah , Ahmad Sohail , Khan Mansoor , Khan Fayaz Ahmad , Khan Wisal , Khan Jehanzeb 
Published: October 2021

Abstract
Investigating the effects of climate and land-use changes on surface runoff is critical for water resources management. The majority of studies focused on projected climate change effects on surface runoff, while neglecting future land-use change. Therefore, the main aim of this article is to discriminate the impacts of projected climate and land-use changes on surface runoff using the Soil and Water Assessment Tool (SWAT) through the lens of the Upper Indus Basin, Pakistan. Future scenarios of the land-use and climate changes are predicted using cellular automata artificial neural network and four bias-corrected general circulation models, respectively. The historical record (2000–2013) was divided into the calibration period (2000–2008) and the validation period (2009–2013). The simulated results demonstrated that the SWAT model performed well. The results obtained from 2000 to 2013 show that climate change (61.61%) has a higher influence on river runoff than land-use change (38.39%). Both climate and land-use changes are predicted to increase future runoff depth in this basin. The influence of climate change (12.76–25.92%) is greater than land-use change (0.37–1.1%). Global weather data has good applicability for simulating hydrological responses in the region where conventional gauges are unavailable. The study discusses that both climate and land-use changes impact runoff depth and concludes with some suggestions for water resources managers to bring water environment sustainability.
Evaluation of power generation and treatment efficiency of dairy wastewater in microbial fuel cell using TiO2 – SPEEK as proton exchange membrane
Authors: Vidhyeswari D. , Surendhar A. , Bhuvaneshwari S. 
Published: October 2021

Abstract
The aim of this study is to synthesise SPEEK composite proton exchange membrane with the addition of TiO2 nanofillers for microbial fuel cell application. SPEEK composite membrane with varying weight percentage of TiO2 (2.5, 5, 7.5 and 10%) was prepared to study the effect of TiO2 concentration on membrane performance. Synthesized composite membranes were subjected to various characterization studies such as FT-IR, XRD, Raman spectroscopy, TGA, UTM and SEM. Physico-chemical properties of membrane such as water uptake capacity, ion exchange capacity and thickness were also analyzed. 5% TiO2 – SPEEK composite membrane exhibited the higher water uptake capacity value and Ion exchange capacity value of 31% and 1.71 meq/g respectively. Performance of the MFC system with TiO2 – SPEEK membranes were evaluated and compared with the pristine SPEEK and Nafion membrane. 5% TiO2 – SPEEK membrane produced the higher power density (1.22 W/m2) and voltage (0.635 V) than the other membranes investigated. Efficacy of MFC in wastewater treatment was evaluated based on the chemical oxygen demand (COD), total organic carbon content and turbidity. Biofilm growth over the surface of the electrodes was also analyzed using scanning electron microscopy.
Selective Estrogen Receptor Modulators (SERMs) Synergize with Cisplatin, Induce Apoptosis and Suppress Cellular Migration and Colony Formation of Lung Cancer Cells
Authors: Alsous Lina , Bardaweel Sanaa 
Published: October 2021

Background:Lung cancer remains the leading cause of cancer-related deaths worldwide. Hence, novel therapeutic approaches targeting crucial pathways are needed to improve its treatment. Previous studies have verified the involvement of the estrogen pathway, mediated through estrogen receptor β (ERβ), in the development and progression of lung carcinogenesis. Selective estrogen receptor modulators (SERMs) are a group of estrogen receptor agonists/antagonists that have tissue selective effects. Many of the available SERMs are used for the management of breast cancer. However, their role in lung cancer is still under investigation.Objectives:The aim of this research is to investigate the anti-tumorigenic activity of the selective estrogen receptor modulators, tamoxifen, raloxifene, and toremifene, against different lung cancer cell lines.Methods:The anti-proliferative and combined effects of SERMs with standard chemotherapy were evaluated by MTT assay. Cell migration was assessed using a wound-healing assay. The mechanism of cell death was determined using the Annexin V-FITC/ propidium iodide staining flow cytometry method. Cells’ capability to form colonies was evaluated by soft agar colony formation assay. Estrogen receptors expression was determined using real-time PCR.Results:Our results have demonstrated the presence of ERβ in A549, H1299, and H661 lung cancer cells. Cellular proliferation assay suggested that SERMs have significantly reduced lung cancer cells proliferation in a time and concentration- dependent manner. Additionally, SERMs exhibited a synergistic effect against A549 cells when combined with cisplatin. SERMs treatment have increased cell apoptosis and resulted in concentration-dependent inhibition of cell migration and colony formation of A549 cells.Conclusion:Selective estrogen receptor modulators may possess potential therapeutic utility for the treatment of lung cancer as monotherapy or in combination with standard chemotherapy.
Synthesis, Molecular Docking, and Biological Evaluation of 2,3-Diphenylquinoxaline
Derivatives as a Tubulin’s Colchicine Binding Site Inhibitor Based on Primary Virtual
Screening
Authors: Amanlou Massoud , Rezaei Zahra , Asadi Mehdi , Montazer Mohammad Nazari , Rezaeiamiri Elnaz , Bahadorikhalili Saeed , Amini Mohsen 
Published: October 2021

Background and Objective:
Tubulin inhibitors have proved to be a promising treatment against cancer.
Tubulin inhibitors target different areas in microtubule structure to exert their effects. The colchicine binding site
(CBS) is one of them for which there is no FDA-approved drug yet. This makes CBS a desirable target for drug design.

Materials and Methods:
Primary virtual screening is done by developing a possible pharmacophore model of colchicine
binding site inhibitors of tubulins, and 2,3-diphenylquinoxaline is chosen as a lead compound to synthesis. In this
study, 28 derivatives of 2,3-diphenylquinoxalines are synthesized, and their cytotoxicity is evaluated by the MTT assay
in different human cancer cell lines, including AGS (Adenocarcinoma gastric cell line), HT-29 (Human colorectal
adenocarcinoma cell line), NIH3T3 (Fibroblast cell line), and MCF-7 (Human breast cancer cell).

Results:
Furthermore, the activity of the studied compounds was investigated using computational methods involving
molecular docking of the 2,3-diphenylquinoxaline derivatives to β-tubulin. The results showed that the compounds
with electron donor functionalities in positions 2 and 3 and electron-withdrawing groups in position 6 are the most
active tubulin inhibitors.

Conclusion:
Apart from the high activity of the synthesized compounds, the advantage of this report is the ease of the
synthesis, work-up, and isolation of the products in safe, effective, and high-quality isolated yields.