bluuebunny/crossref_metadata_2025_split huggingface dataset:
The epidemiology of Plasmodium vivax among adults in the Democratic Republic of the Congo
Authors: Brazeau Nicholas F. , Mitchell Cedar L. , Morgan Andrew P. , Deutsch-Feldman Molly , Watson Oliver John , Thwai Kyaw L. , Gelabert Pere , van Dorp Lucy , Keeler Corinna Y. , Waltmann Andreea , Emch Michael , Gartner Valerie , Redelings Ben , Wray Gregory A. , Mwandagalirwa Melchior K. , Tshefu Antoinette K. , Likwela Joris L. , Edwards Jessie K. , Verity Robert , Parr Jonathan B. , Meshnick Steven R. , Juliano Jonathan J. 
Published: July 2021

AbstractReports of P. vivax infections among Duffy-negative hosts have accumulated throughout sub-Saharan Africa. Despite this growing body of evidence, no nationally representative epidemiological surveys of P. vivax in sub-Saharan Africa have been performed. To overcome this gap in knowledge, we screened over 17,000 adults in the Democratic Republic of the Congo (DRC) for P. vivax using samples from the 2013-2014 Demographic Health Survey. Overall, we found a 2.97% (95% CI: 2.28%, 3.65%) prevalence of P. vivax infections across the DRC. Infections were associated with few risk-factors and demonstrated a relatively flat distribution of prevalence across space with focal regions of relatively higher prevalence in the north and northeast. Mitochondrial genomes suggested that DRC P. vivax were distinct from circulating non-human ape strains and an ancestral European P. vivax strain, and instead may be part of a separate contemporary clade. Our findings suggest P. vivax is diffusely spread across the DRC at a low prevalence, which may be associated with long-term carriage of low parasitemia, frequent relapses, or a general pool of infections with limited forward propagation.
Vectorial Doppler metrology
Authors: Fang Liang , Wan Zhenyu , Forbes Andrew , Wang Jian 
Published: July 2021

AbstractThe Doppler effect is a universal wave phenomenon that has spurred a myriad of applications. In early manifestations, it was implemented by interference with a reference wave to infer linear velocities along the direction of motion, and more recently lateral and angular velocities using scalar phase structured light. A consequence of the scalar wave approach is that it is technically challenging to directly deduce the motion direction of moving targets. Here we overcome this challenge using vectorially structured light with spatially variant polarization, allowing the velocity and motion direction of a moving particle to be fully determined. Using what we call a vectorial Doppler effect, we conduct a proof of principle experiment and successfully measure the rotational velocity (magnitude and direction) of a moving isotropic particle. The instantaneous position of the moving particle is also tracked under the conditions of knowing its starting position and continuous tracking. Additionally, we discuss its applicability to anisotropic particle detection, and show its potential to distinguish the rotation and spin of the anisotropic particle and measure its rotational velocity and spin speed (magnitude and direction). Our demonstration opens the path to vectorial Doppler metrology for detection of universal motion vectors with vectorially structured light.
M1BP cooperates with CP190 to activate transcription at TAD borders and promote chromatin insulator activity
Authors: Bag Indira , Chen Shue , Rosin Leah F. , Chen Yang , Liu Chen-Yu , Yu Guo-Yun , Lei Elissa P. 
Published: July 2021

AbstractGenome organization is driven by forces affecting transcriptional state, but the relationship between transcription and genome architecture remains unclear. Here, we identified the Drosophila transcription factor Motif 1 Binding Protein (M1BP) in physical association with the gypsy chromatin insulator core complex, including the universal insulator protein CP190. M1BP is required for enhancer-blocking and barrier activities of the gypsy insulator as well as its proper nuclear localization. Genome-wide, M1BP specifically colocalizes with CP190 at Motif 1-containing promoters, which are enriched at topologically associating domain (TAD) borders. M1BP facilitates CP190 chromatin binding at many shared sites and vice versa. Both factors promote Motif 1-dependent gene expression and transcription near TAD borders genome-wide. Finally, loss of M1BP reduces chromatin accessibility and increases both inter- and intra-TAD local genome compaction. Our results reveal physical and functional interaction between CP190 and M1BP to activate transcription at TAD borders and mediate chromatin insulator-dependent genome organization.
Oropharyngeal cancer patient stratification using random forest based-learning over high-dimensional radiomic features
Authors: Patel Harsh , Vock David M. , Marai G. Elisabeta , Fuller Clifton D. , Mohamed Abdallah S. R. , Canahuate Guadalupe 
Published: July 2021

AbstractTo improve risk prediction for oropharyngeal cancer (OPC) patients using cluster analysis on the radiomic features extracted from pre-treatment Computed Tomography (CT) scans. 553 OPC Patients randomly split into training (80%) and validation (20%), were classified into 2 or 3 risk groups by applying hierarchical clustering over the co-occurrence matrix obtained from a random survival forest (RSF) trained over 301 radiomic features. The cluster label was included together with other clinical data to train an ensemble model using five predictive models (Cox, random forest, RSF, logistic regression, and logistic-elastic net). Ensemble performance was evaluated over the independent test set for both recurrence free survival (RFS) and overall survival (OS). The Kaplan–Meier curves for OS stratified by cluster label show significant differences for both training and testing (p val < 0.0001). When compared to the models trained using clinical data only, the inclusion of the cluster label improves AUC test performance from .62 to .79 and from .66 to .80 for OS and RFS, respectively. The extraction of a single feature, namely a cluster label, to represent the high-dimensional radiomic feature space reduces the dimensionality and sparsity of the data. Moreover, inclusion of the cluster label improves model performance compared to clinical data only and offers comparable performance to the models including raw radiomic features.
Biofilm inhibition and bactericidal activity of NiTi alloy coated with graphene oxide/silver nanoparticles via electrophoretic deposition
Authors: Pipattanachat Sirapat , Qin Jiaqian , Rokaya Dinesh , Thanyasrisung Panida , Srimaneepong Viritpon 
Published: July 2021

AbstractBiofilm formation on medical devices can induce complications. Graphene oxide/silver nanoparticles (GO/AgNPs) coated nickel-titanium (NiTi) alloy has been successfully produced. Therefore, the aim of this study was to determine the anti-bacterial and anti-biofilm effects of a GO/AgNPs coated NiTi alloy prepared by Electrophoretic deposition (EPD). GO/AgNPs were coated on NiTi alloy using various coating times. The surface characteristics of the coated NiTi alloy substrates were investigated and its anti-biofilm and anti-bacterial effect on Streptococcus mutans biofilm were determined by measuring the biofilm mass and the number of viable cells using a crystal violet assay and colony counting assay, respectively. The results showed that although the surface roughness increased in a coating time-dependent manner, there was no positive correlation between the surface roughness and the total biofilm mass. However, increased GO/AgNPs deposition produced by the increased coating time significantly reduced the number of viable bacteria in the biofilm (p < 0.05). Therefore, the GO/AgNPs on NiTi alloy have an antibacterial effect on the S. mutans biofilm. However, the increased surface roughness does not influence total biofilm mass formation (p = 0.993). Modifying the NiTi alloy surface using GO/AgNPs can be a promising coating to reduce the consequences of biofilm formation.
Paternal and maternal long-term psychological outcomes after uterine artery embolization for severe post-partum hemorrhage
Authors: Bernasconi Maude , Eggel-Hort Béatrice , Horsch Antje , Vial Yvan , Denys Alban , Quibel Thibaud , Baud David 
Published: July 2021

AbstractThis study intend to compare the long-term psychological impact (depression, post-traumatic stress disorder) on both partners between patients that underwent uterine artery embolization (UAE) for post-partum hemorrhage (PPH) and uneventful deliveries. Women who experienced severe PPH treated by UAE in our institution between 2003 and 2013 were identified in our obstetrical database. These cases were matched to controls with uneventful deliveries. Matching criteria were maternal age, parity, ethnicity, year of delivery, birthweight, gestational age and mode of delivery. Patients and their partners completed validated questionnaires measuring post-traumatic stress (TSQ), as well as depression symptoms (MINI). A total of 63 cases of PPH and 189 matched controls (1:3) participated in a study exploring gynecological and obstetrical outcomes. With a mean of 8 years post-index delivery, patients after PPH showed increased risk of depression (p = 0.015) and post-traumatic stress disorder (22.2% versus 4.8%, p < 0.005) compared to controls. PPH remains strongly associated with post-traumatic stress disorder, even after adjustment for depression (adjusted odds ratio 5.1; 95% confidence intervals 1.5–17.5). Similarly, partners of patients with PPH showed a propensity to depression (p = 0.029) and post-traumatic stress disorder (11.5% versus 1.5%, p = 0.019). In conclusion, both women and their partners are at increased risk of long-term psychological adverse outcomes after PPH. Couples may benefit from psychological support.
Fine adaptive precision grip control without maximum pinch strength changes after upper limb neurodynamic mobilization
Authors: Dierick Frédéric , Brismée Jean-Michel , White Olivier , Bouché Anne-France , Périchon Céline , Filoni Nastasia , Barvaux Vincent , Buisseret Fabien 
Published: July 2021

AbstractBefore and immediately after passive upper limb neurodynamic mobilizations targeting the median nerve, grip ($$G_F$$

G
F

) and load ($$L_F$$

L
F

) forces applied by the thumb, index and major fingers (three-jaw chuck pinch) were collected using a manipulandum during three different grip precision tasks: grip-lift-hold-replace (GLHR), vertical oscillations (OSC), and vertical oscillations with up and down collisions (OSC/COLL/u, OSC/COLL/d). Several parameters were collected or computed from $$G_F$$

G
F

and $$L_F$$

L
F

. Maximum pinch strength and fingertips pressure sensation threshold were also examined. After the mobilizations, $$L_F$$

L
F

max changes from 3.2 ± 0.4 to 3.4 ± 0.4 N (p = 0.014), d$$G_F$$

G
F

from 89.0 ± 66.6 to 102.2 ± 59.6 $$N~\text{s}^{-1}$$

N

s

-
1

(p = 0.009), and d$$L_F$$

L
F

from 43.6 ± 17.0 to 56.0 ± 17.9 $$N~\text{s}^{-1}$$

N

s

-
1

($$p<$$

p
<

0.001) during GLHR. $$L_F$$

L
F

SD changes from 0.9 ± 0.3 to 1.0 ± 0.2 N (p = 0.004) during OSC. $$L_F$$

L
F

peak changes from 17.4 ± 8.3 to 15.1 ± 7.5 N ($$p<$$

p
<

0.001), $$G_F$$

G
F

from 12.4 ± 6.7 to 11.3 ± 6.8 N (p = 0.033), and $$L_F$$

L
F

from 2.9 ± 0.4 to 3.00 ± 0.4 N (p = 0.018) during OSC/COLL/u. $$G_F$$

G
F

peak changes from 13.5 ± 7.4 to 12.3 ± 7.7 N (p = 0.030) and $$L_F$$

L
F

from 14.5 ± 6.0 to 13.6 ± 5.5 N (p = 0.018) during OSC/COLL/d. Sensation thresholds at index and thumb were reduced (p = 0.001, p = 0.008). Precision grip adaptations observed after the mobilizations could be partly explained by changes in cutaneous median-nerve pressure afferents from the thumb and index fingertips.
Understanding the effect of smoking and drinking behavior on Parkinson's disease risk: a Mendelian randomization study
Authors: Domínguez-Baleón Carmen , Ong Jue-Sheng , Scherzer Clemens R. , Rentería Miguel E. , Dong Xianjun 
Published: July 2021

AbstractPrevious observational studies have identified correlations between Parkinson’s disease (PD) risk and lifestyle factors. However, whether or not those associations are causal remains unclear. To infer causality between PD risk and smoking or alcohol intake, we conducted a two-sample Mendelian randomization study using genome-wide association study summary statistics from the GWAS & Sequencing Consortium of Alcohol and Nicotine use study (1.2 million participants) and the latest meta-analysis from the International Parkinson’s Disease Genomics Consortium (37,688 PD cases and 18,618 proxy-cases). We performed sensitivity analyses, including testing for pleiotropy with MR-Egger and MR-PRESSO, and multivariable MR modeling to account for the genetic effects of competing substance use traits on PD risk. Our results revealed causal associations ofalcohol intake(OR 0.79; 95% CI 0.65–0.96; p = 0.021) andsmoking continuation(which comparescurrentvs.former smokers) (OR 0.64; 95% CI 0.46–0.89; p = 0.008) with lower PD risk. Multivariable MR analyses showed that the causal association betweendrinks per weekand PD is unlikely due to confounding by smoking behavior. Finally, frailty analyses suggested that the causal effects of bothalcohol intakeandsmoking continuationon PD risk estimated from MR analysis are not explained by the presence of survival bias alone. Our findings support the role of smoking as a protective factor against PD, but only when comparingcurrent vs. former smokers. Similarly, increased alcohol intake had a protective effect over PD risk, with thealcohol dehydrogenase 1B(ADH1B) locus as a potential candidate for further investigation of the mechanisms underlying this association.
Twenty-first-century projections of shoreline change along inlet-interrupted coastlines
Authors: Bamunawala Janaka , Ranasinghe Roshanka , Dastgheib Ali , Nicholls Robert J. , Murray A. Brad , Barnard Patrick L. , Sirisena T. A. J. G. , Duong Trang Minh , Hulscher Suzanne J. M. H. , van der Spek Ad 
Published: July 2021

AbstractSandy coastlines adjacent to tidal inlets are highly dynamic and widespread landforms, where large changes are expected due to climatic and anthropogenic influences. To adequately assess these important changes, both oceanic (e.g., sea-level rise) and terrestrial (e.g., fluvial sediment supply) processes that govern the local sediment budget must be considered. Here, we present novel projections of shoreline change adjacent to 41 tidal inlets around the world, using a probabilistic, reduced complexity, system-based model that considers catchment-estuary-coastal systems in a holistic way. Under the RCP 8.5 scenario, retreat dominates (90% of cases) over the twenty-first century, with projections exceeding 100 m of retreat in two-thirds of cases. However, the remaining systems are projected to accrete under the same scenario, reflecting fluvial influence. This diverse range of response compared to earlier methods implies that erosion hazards at inlet-interrupted coasts have been inadequately characterised to date. The methods used here need to be applied widely to support evidence-based coastal adaptation.
Enhanced extraction of skin interstitial fluid using a 3D printed device enabling tilted microneedle penetration
Authors: Kim Sanha , Lee Min Suk , Yang Hee Seok , Jung Jae Hwan 
Published: July 2021

AbstractInterstitial fluid (ISF) is a body fluid that fills, surrounds cells and contains various biomarkers, but it has been challenging to extract ISF in a reliable and sufficient amount with high speed. To address the issues, we developed the tilted microneedle ISF collecting system (TMICS) fabricated by 3D printing. In this system, the microneedle (MN) was inserted at 66° to the skin by TMICS so that the MN length could be extended within a safe range of skin penetration. Moreover, TMICS incorporating three MN patches created reliable ISF collecting conditions by penetrating the skin at consistent angle and force, 4.9 N. Due to the MN length increase and the patch number expansion, the surface area of the penetrated tissue was increased, thereby confirming that ISF extraction efficiency was improved. Skin ISF was collected into the paper reservoir on the patch, and the absorbed area was converted into a volume. ISF extraction from the rat skin in vivo by TMICS was well tolerated, and the 2.9 μL of ISF was obtained within 30 s. Therefore, TMICS is promising to apply in the diagnosis of multiple biomarkers in ISF with high speed and stability.
Flexible time course of spatial frequency use during scene categorization
Authors: Wiesmann Sandro L. , Caplette Laurent , Willenbockel Verena , Gosselin Frédéric , Võ Melissa L.-H. 
Published: July 2021

AbstractHuman observers can quickly and accurately categorize scenes. This remarkable ability is related to the usage of information at different spatial frequencies (SFs) following a coarse-to-fine pattern: Low SFs, conveying coarse layout information, are thought to be used earlier than high SFs, representing more fine-grained information. Alternatives to this pattern have rarely been considered. Here, we probed all possible SF usage strategies randomly with high resolution in both the SF and time dimensions at two categorization levels. We show that correct basic-level categorizations of indoor scenes are linked to the sampling of relatively high SFs, whereas correct outdoor scene categorizations are predicted by an early use of high SFs and a later use of low SFs (fine-to-coarse pattern of SF usage). Superordinate-level categorizations (indoor vs. outdoor scenes) rely on lower SFs early on, followed by a shift to higher SFs and a subsequent shift back to lower SFs in late stages. In summary, our results show no consistent pattern of SF usage across tasks and only partially replicate the diagnostic SFs found in previous studies. We therefore propose that SF sampling strategies of observers differ with varying stimulus and task characteristics, thus favouring the notion of flexible SF usage.
Metformin-induced ROS upregulation as amplified by apigenin causes profound anticancer activity while sparing normal cells
Authors: Warkad Madhuri Shende , Kim Chea-Ha , Kang Beom-Goo , Park Soo-Hyun , Jung Jun-Sub , Feng Jing-Hui , Inci Gozde , Kim Sung-Chan , Suh Hong-Won , Lim Soon Sung , Lee Jae-Yong 
Published: July 2021

AbstractMetformin increased cellular ROS levels in AsPC-1 pancreatic cancer cells, with minimal effect in HDF, human primary dermal fibroblasts. Metformin reduced cellular ATP levels in HDF, but not in AsPC-1 cells. Metformin increased AMPK, p-AMPK (Thr172), FOXO3a, p-FOXO3a (Ser413), and MnSOD levels in HDF, but not in AsPC-1 cells. p-AMPK and p-FOXO3a also translocated from the cytosol to the nucleus by metformin in HDF, but not in AsPC-1 cells. Transfection of si-FOXO3a in HDF increased ROS levels, while wt-FOXO3a-transfected AsPC-1 cells decreased ROS levels. Metformin combined with apigenin increased ROS levels dramatically and decreased cell viability in various cancer cells including AsPC-1 cells, with each drug used singly having a minimal effect. Metformin/apigenin combination synergistically decreased mitochondrial membrane potential in AsPC-1 cells but to a lesser extent in HDF cells. Metformin/apigenin combination in AsPC-1 cells increased DNA damage-, apoptosis-, autophagy- and necroptosis-related factors, but not in HDF cells. Oral administration with metformin/apigenin caused dramatic blocks tumor size in AsPC-1-xenografted nude mice. Our results suggest that metformin in cancer cells differentially regulates cellular ROS levels via AMPK-FOXO3a-MnSOD pathway and combination of metformin/apigenin exerts anticancer activity through DNA damage-induced apoptosis, autophagy and necroptosis by cancer cell-specific ROS amplification.
Evaluation of non-thermal effect of microwave radiation and its mode of action in bacterial cell inactivation
Authors: Shaw Priyanka , Kumar Naresh , Mumtaz Sohail , Lim Jun Sup , Jang Jung Hyun , Kim Doyoung , Sahu Bidya Dhar , Bogaerts Annemie , Choi Eun Ha 
Published: July 2021

AbstractA growing body of literature has recognized the non-thermal effect of pulsed microwave radiation (PMR) on bacterial systems. However, its mode of action in deactivating bacteria has not yet been extensively investigated. Nevertheless, it is highly important to advance the applications of PMR from simple to complex biological systems. In this study, we first optimized the conditions of the PMR device and we assessed the results by simulations, using ANSYS HFSS (High Frequency Structure Simulator) and a 3D particle-in-cell code for the electron behavior, to provide a better overview of the bacterial cell exposure to microwave radiation. To determine the sensitivity of PMR, Escherichia coli and Staphylococcus aureus cultures were exposed to PMR (pulse duration: 60 ns, peak frequency: 3.5 GHz) with power density of 17 kW/cm2 at the free space of sample position, which would induce electric field of 8.0 kV/cm inside the PBS solution of falcon tube in this experiment at 25 °C. At various discharges (D) of microwaves, the colony forming unit curves were analyzed. The highest ratios of viable count reductions were observed when the doses were increased from 20D to 80D, which resulted in an approximate 6 log reduction in E. coli and 4 log reduction in S. aureus. Moreover, scanning electron microscopy also revealed surface damage in both bacterial strains after PMR exposure. The bacterial inactivation was attributed to the deactivation of oxidation-regulating genes and DNA damage.
Effect of strain-induced anisotropy on magnetization dynamics in Y3Fe5O12 films recrystallized on a lattice-mismatched substrate
Authors: Krysztofik Adam , Özoğlu Sevgi , McMichael Robert D. , Coy Emerson 
Published: July 2021

AbstractWe report on the correlation of structural and magnetic properties of Y3Fe5O12 (YIG) films deposited on Y3Al5O12 substrates using pulsed laser deposition. The recrystallization process leads to an unexpected formation of interfacial tensile strain and consequently strain-induced anisotropy contributing to the perpendicular magnetic anisotropy. The ferromagnetic resonance linewidth of YIG is significantly increased in comparison to a film on a lattice-matched Gd3Ga5O12 substrate. Notably, the linewidth dependency on frequency has a negative slope. The linewidth behavior is explained with the proposed anisotropy dispersion model.
Surveillance of West Nile virus infection in Kashgar Region, Xinjiang, China, 2013–2016
Authors: Zhang Yanping , Lei Wenwen , Wang Yali , Sui Haitian , Liu Bo , Li Fan , He Ying , Li Zhaoxia , Fu Shihong , Wang Lu , Xu Limin , Mahe Muti , Gao Zhenguo , Mamutijiang Tuerxun , Lv Zhi , Xiang Nijuan , Zhou Lei , Ni Daxin , Liang Guodong , Li Qun , Wang Huanyu , Feng Zijian 
Published: July 2021

AbstractWest Nile virus (WNV) was first isolated in mainland China from mosquitoes in Jiashi County, Kashgar Region, Xinjiang in 2011, following local outbreaks of viral meningitis and encephalitis caused by WNV. To elaborate the epidemiological characteristics of the WNV, surveillance of WNV infection in Kashgar Region, Xinjiang from 2013 to 2016 were carried out. Blood and CSF samples from surveillance human cases, blood of domestic chicken, cattle, sheep and mosquitoes in Kashgar Region were collected and detected. There were human 65 WNV Immunoglobulin M (IgM) antibody positive cases by ELISA screening, 6 confirmed WNV cases by the plaque reduction neutralization test (PRNT) screening. These cases occurred mainly concentrated in August to September of each year, and most of them were males. WNV-neutralizing antibodies were detected in both chickens and sheep, and the positive rates of neutralizing antibodies were 15.5% and 1.78%, respectively. A total of 15,637 mosquitoes were collected in 2013–2016, with Culex pipiens as the dominant mosquito species. Four and 1 WNV-positive mosquito pools were detected by RT-qPCR in 2013 and 2016 respectively. From these data, we can confirm that Jiashi County may be a natural epidemic foci of WNV disease, the trend highlights the routine virology surveillance in WNV surveillance cases, mosquitoes and avian should be maintained and enhanced to provide to prediction and early warning of outbreak an epidemic of WNV in China.