bluuebunny/crossref_metadata_2025_split huggingface dataset:
Global Population Genetic Structure and Male-Mediated Gene Flow in the Green Sea Turtle (Chelonia mydas): Analysis of Microsatellite Loci
Authors: Roberts Mark A , Schwartz Tonia S , Karl Stephen A 
Published: April 2021

Abstract
We assessed the degree of population subdivision among global populations of green sea turtles, Chelonia mydas, using four microsatellite loci. Previously, a single-copy nuclear DNA study indicated significant male-mediated gene flow among populations alternately fixed for different mitochondrial DNA haplotypes and that genetic divergence between populations in the Atlantic and Pacific Oceans was more common than subdivisions among populations within ocean basins. Even so, overall levels of variation at single-copy loci were low and inferences were limited. Here, the markedly more variable microsatellite loci confirm the presence of male-mediated gene flow among populations within ocean basins. This analysis generally confirms the genetic divergence between the Atlantic and Pacific. As with the previous study, phylogenetic analyses of genetic distances based on the microsatellite loci indicate a close genetic relationship among eastern Atlantic and Indian Ocean populations. Unlike the single-copy study, however, the results here cannot be attributed to an artifact of general low variability and likely represent recent or ongoing migration between ocean basins. Sequence analyses of regions flanking the microsatellite repeat reveal considerable amounts of cryptic variation and homoplasy and significantly aid in our understanding of population connectivity. Assessment of the allele frequency distributions indicates that at least some of the loci may not be evolving by the stepwise mutation model.
Evolutionary Relationships of Major Histocompatibility Complex Class I Genes in Simian Primates
Authors: Sawai Hiromi , Kawamoto Yoshi , Takahata Naoyuki , Satta Yoko 
Published: April 2021

AbstractNew World monkeys (NWMs) occupy a critical phylogenetic position in elucidating the evolutionary process of major histocompatibility complex (MHC) class I genes in primates. From three subfamilies of Aotinae, Cebinae, and Atelinae, the 5′-flanking regions of 18 class I genes are obtained and phylogenetically examined in terms of Alu/LINE insertion elements as well as the nucleotide substitutions. Two pairs of genes from Aotinae and Atelinae are clearly orthologous to human leukocyte antigen (HLA) -E and -F genes. Of the remaining 14 genes, 8 belong to the distinct group B, together with HLA-B and -C, to the exclusion of all other HLA class I genes. These NWM genes are classified into four groups, designated as NWM-B1, -B2, -B3, and -B4. Of these, NWM-B2 is orthologous to HLA-B/C. Also, orthologous relationships of NWM-B1, -B2, and -B3 exist among different families of Cebidae and Atelidae, which is in sharp contrast to the genus-specific gene organization within the subfamily Callitrichinae. The other six genes belong to the distinct group G. However, a clade of these NWM genes is almost equally related to HLA-A, -J, -G, and -K, and there is no evidence for their orthologous relationships to HLA-G. It is argued that class I genes in simian primates duplicated extensively in their common ancestral lineage and that subsequent evolution in descendant species has been facilitated mainly by independent loss of genes.
Integrating Genetic Linkage Maps With Pachytene Chromosome Structure in Maize
Authors: Anderson Lorinda K , Salameh Naser , Bass Hank W , Harper Lisa C , Cande W Z , Weber Gerd , Stack Stephen M 
Published: April 2021

Abstract
Genetic linkage maps reveal the order of markers based on the frequency of recombination between markers during meiosis. Because the rate of recombination varies along chromosomes, it has been difficult to relate linkage maps to chromosome structure. Here we use cytological maps of crossing over based on recombination nodules (RNs) to predict the physical position of genetic markers on each of the 10 chromosomes of maize. This is possible because (1) all 10 maize chromosomes can be individually identified from spreads of synaptonemal complexes, (2) each RN corresponds to one crossover, and (3) the frequency of RNs on defined chromosomal segments can be converted to centimorgan values. We tested our predictions for chromosome 9 using seven genetically mapped, single-copy markers that were independently mapped on pachytene chromosomes using in situ hybridization. The correlation between predicted and observed locations was very strong (r2 = 0.996), indicating a virtual 1:1 correspondence. Thus, this new, high-resolution, cytogenetic map enables one to predict the chromosomal location of any genetically mapped marker in maize with a high degree of accuracy. This novel approach can be applied to other organisms as well.
Genomic Background Predicts the Fate of Duplicated Genes: Evidence From the Yeast Genome
Authors: Zhang Ze , Kishino Hirohisa 
Published: April 2021

Abstract
Gene duplication with subsequent divergence plays a central role in the acquisition of genes with novel function and complexity during the course of evolution. With reduced functional constraints or through positive selection, these duplicated genes may experience accelerated evolution. Under the model of subfunctionalization, loss of subfunctions leads to complementary acceleration at sites with two copies, and the difference in average rate between the sequences may not be obvious. On the other hand, the classical model of neofunctionalization predicts that the evolutionary rate in one of the two duplicates is accelerated. However, the classical model does not tell which of the duplicates experiences the acceleration in evolutionary rate. Here, we present evidence from the Saccharomyces cerevisiae genome that a duplicate located in a genomic region with a low-recombination rate is likely to evolve faster than a duplicate in an area of high recombination. This observation is consistent with population genetics theory that predicts that purifying selection is less effective in genomic regions of low recombination (Hill-Robertson effect). Together with previous studies, our results suggest the genomic background (e.g., local recombination rate) as a potential force to drive the divergence between nontandemly duplicated genes. This implies the importance of structure and complexity of genomes in the diversification of organisms via gene duplications.
How to Stick the Landing: Kangaroo Rats Use Their Tails to Reorient during Evasive Jumps Away from Predators
Authors: Schwaner M Janneke , Freymiller Grace A , Clark Rulon W , McGowan Craig P 
Published: April 2021

SynopsisTails are widespread in the animal world and play important roles in locomotor tasks, such as propulsion, maneuvering, stability, and manipulation of objects. Kangaroo rats, bipedal hopping rodents, use their tail for balancing during hopping, but the role of their tail during the vertical evasive escape jumps they perform when attacked by predators is yet to be determined. Because we observed kangaroo rats swinging their tails around their bodies while airborne following escape jumps, we hypothesized that kangaroo rats use their tails to not only stabilize their bodies while airborne, but also to perform aerial re-orientations. We collected video data from free-ranging desert kangaroo rats (Dipodomys deserti) performing escape jumps in response to a simulated predator attack and analyzed the rotation of their bodies and tails in the yaw plane (about the vertical-axis). Kangaroo rat escape responses were highly variable. The magnitude of body re-orientation in yaw was independent of jump height, jump distance, and aerial time. Kangaroo rats exhibited a stepwise re-orientation while airborne, in which slower turning periods corresponded with the tail center of mass being aligned close to the vertical rotation axis of the body. To examine the effect of tail motion on body re-orientation during a jump, we compared average rate of change in angular momentum. Rate of change in tail angular momentum was nearly proportional to that of the body, indicating that the tail reorients the body in the yaw plane during aerial escape leaps by kangaroo rats. Although kangaroo rats make dynamic 3D movements during their escape leaps, our data suggest that kangaroo rats use their tails to control orientation in the yaw plane. Additionally, we show that kangaroo rats rarely use their tail length at full potential in yaw, suggesting the importance of tail movement through multiple planes simultaneously.
Chemical Communication in Lizards and a Potential Role for Vasotocin in Modulating Social Interactions
Authors: Campos Stephanie M , Belkasim Selma S 
Published: April 2021

Synopsis
Lizards use chemical communication to mediate many reproductive, competitive, and social behaviors, but the neuroendocrine mechanisms underlying chemical communication in lizards are not well understood and understudied. By implementing a neuroendocrine approach to the study of chemical communication in reptiles, we can address a major gap in our knowledge of the evolutionary mechanisms shaping chemical communication in vertebrates. The neuropeptide arginine vasotocin (AVT) and its mammalian homolog vasopressin are responsible for a broad spectrum of diversity in competitive and reproductive strategies in many vertebrates, mediating social behavior through the chemosensory modality. In this review, we posit that, though limited, the available data on AVT-mediated chemical communication in lizards reveal intriguing patterns that suggest AVT plays a more prominent role in lizard chemosensory behavior than previously appreciated. We argue that these results warrant more research into the mechanisms used by AVT to modify the performance of chemosensory behavior and responses to conspecific chemical signals. We first provide a broad overview of the known social functions of chemical signals in lizards, the glandular sources of chemical signal production in lizards (e.g., epidermal secretory glands), and the chemosensory detection methods and mechanisms used by lizards. Then, we review the locations of vasotocinergic populations and neuronal projections in lizard brains, as well as sites of peripheral receptors for AVT in lizards. Finally, we end with a case study in green anoles (Anolis carolinensis), discussing findings from recently published work on the impact of AVT in adult males on chemosensory communication during social interactions, adding new data from a similar study in which we tested the impact of AVT on chemosensory behavior of adult females. We offer concluding remarks on addressing several fundamental questions regarding the role of AVT in chemosensory communication and social behavior in lizards.
Dogs as Pets and Pests: Global Patterns of Canine Abundance, Activity, and Health
Authors: Bryce Caleb M 
Published: April 2021

SynopsisDogs (Canis familiaris) were the first domesticated species and, at an estimated population of 1 billion individuals, are globally ubiquitous today. Describing the tremendous morphometric diversity and evolutionary origins of dogs is a scientific endeavor that predates Darwin, yet our interdisciplinary understanding of the species is just beginning. Here, I present global trends in dog abundance, activity, and health. While the human–dog relationship has for millennia been close, it is also complicated. As pets, companion dogs are often treated as family members and constitute the largest sector of the ever-growing &gt;$200 billion USD global pet care industry. As pests, free-roaming dogs are an emerging threat to native species via both predation and nonconsumptive effects (e.g., disturbance, competition for resources, and hybridization). Furthermore, I briefly discuss mounting evidence of dogs as not only infectious disease reservoirs but also as bridges for the transmission of pathogens between wild animals and humans in zoonotic spillover events, triggering intensive dog population management strategies such as culling. Dog mobility across the urban-wildland interface is an important driver for this and other adverse effects of canines on wildlife populations and is an active topic of disease ecologists and conservation biologists. Other canine scientists, including veterinary clinicians and physiologists, study more mechanistic aspects of dog mobility: the comparative kinetics, kinematics, and energetics of dog locomotor health. I outline the prevalent methodological approaches and breed-specific findings within dog activity and health research, then conclude by recognizing promising technologies that are bridging disciplinary gaps in canine science.
Centering Microbes in the Emerging Role of Integrative Biology in Understanding Environmental Change
Authors: Weems Ebony I , de la Sancha Noé U , Anderson Laurel J , Zambrana-Torrelio Carlos , Ferraris Ronaldo P 
Published: April 2021

Abstract
The current environmental changes stressing the Earth’s biological systems urgently require study from an integrated perspective to reveal unexpected, cross-scale interactions, particularly between microbes and macroscale phenomena. Such interactions are the basis of a mechanistic understanding of the important connections between deforestation and emerging infectious disease, feedback between ecosystem disturbance and the gut microbiome, and the cross-scale effects of environmental pollutants. These kinds of questions can be answered with existing techniques and data, but a concerted effort is necessary to better coordinate studies and data sets from different disciplines to fully leverage their potential.
A convergent finite element algorithm for generalized mean curvature flows of closed surfaces
Authors: Binz Tim , Kovács Balázs 
Published: April 2021

Abstract
An algorithm is proposed for generalized mean curvature flow of closed two-dimensional surfaces, which include inverse mean curvature flow and powers of mean and inverse mean curvature flow. Error estimates are proved for semidiscretizations and full discretizations for the generalized flow. The algorithm proposed and studied here combines evolving surface finite elements, whose nodes determine the discrete surface, and linearly implicit backward difference formulae for time integration. The numerical method is based on a system coupling the surface evolution to nonlinear second-order parabolic evolution equations for the normal velocity and normal vector. A convergence proof is presented in the case of finite elements of polynomial degree at least 2 and backward difference formulae of orders 2 to 5. The error analysis combines stability estimates and consistency estimates to yield optimal-order $H^1$-norm error bounds for the computed surface position, velocity, normal vector, normal velocity and therefore for the mean curvature. The stability analysis is performed in the matrix–vector formulation and is independent of geometric arguments, which only enter the consistency analysis. Numerical experiments are presented to illustrate the convergence results and also to report on monotone quantities, e.g. Hawking mass for inverse mean curvature flow, and complemented by experiments for nonconvex surfaces.
A likelihood-based convolution approach to estimate major health events in longitudinal health records data: an external validation study
Authors: Pruinelli Lisiane , Zhou Jiaqi , Stai Bethany , Schold Jesse D , Pruett Timothy , Ma Sisi , Simon Gyorgy 
Published: April 2021

Abstract

Objective
In electronic health record data, the exact time stamp of major health events, defined by significant physiologic or treatment changes, is often missing. We developed and externally validated a method that can accurately estimate these time stamps based on accurate time stamps of related data elements.

Materials and Methods
A novel convolution-based change detection methodology was developed and tested using data from the national deidentified clinical claims OptumLabs data warehouse, then externally validated on a single center dataset derived from the M Health Fairview system.

Results
We applied the methodology to estimate time to liver transplantation for waitlisted candidates. The median error between estimated date within the period of the actual true date was zero days, and median error was 92% and 84% of the transplants, in development and validation samples, respectively.

Discussion
The proposed method can accurately estimate missing time stamps. Successful external validation suggests that the proposed method does not need to be refit to each health system; thus, it can be applied even when training data at the health system is insufficient or unavailable. The proposed method was applied to liver transplantation but can be more generally applied to any missing event that is accompanied by multiple related events that have accurate time stamps.

Conclusion
Missing time stamps in electronic healthcare record data can be estimated using time stamps of related events. Since the model was developed on a nationally representative dataset, it could be successfully transferred to a local health system without substantial loss of accuracy.
Evaluation of Beta-Cyfluthrin Resistance of Cigarette Beetle (Coleoptera: Anobiidae) from Cigarette Manufacturing Factories of China and Underlying Metabolic Mechanisms Responsible for Resistance
Authors: Sun Qian , Chen Xiang , Lin Tao , Cheng Xinsheng 
Published: April 2021

Abstract
Beta-cyfluthrin, as a synthetic pyrethroid, has been widely used in cigarette manufacturing factories in China to control Lasioderma serricorne (F.) (Coleoptera: Anobiidae). In this study, spray toxicity bioassays and filter paper residual contact toxicity bioassays were conducted to investigate the beta-cyfluthrin sensitivity level of five field strains of L. serricorne collected from cigarette manufacturing factories in China. Bioassay results indicated that five field strains had developed different levels of resistance to beta-cyfluthrin with RR50 of 3.51–10.20 at 2 hr after application and 4.05–49.50 at 24 hr after application in spray toxicity bioassays, and RR50 of 4.74–14.47 at 2 hr exposure in filter paper residual contact bioassays. In addition, we examined CarE, GST, and CYP450 enzyme activity and content of L. serricorne adults and larvae. Enzyme-linked immunosorbent assay results suggested that there was no significant difference in GST, CYP450, and CarE content of L. serricorne adults between field strains and reference sensitive strain. Biochemical assay results indicated that CYP450 activity of L. serricorne adults and larvae of five field strains was significantly higher than that of reference sensitive strain, with increased CYP450 activity of 1.08–1.82-fold in adults and 1.08–2.12-fold in larvae. The results implied that elevated CYP450 activity may contribute to metabolic resistance of L. serricorne to pyrethroid. Our study indicated that there was no clear evidence that the enhanced CarE and GST activity was associated with pyrethroid resistance of L. serricorne.
Recalibrating the WTO Dispute Settlement System: Towards New Standards of Appellate Review
Authors: Cottier Thomas 
Published: April 2021

ABSTRACT
This paper analyses the legal nature of the dispute settlement system in the World Trade Organization. It places the Panel stage at the heart of it and stresses the crucial role of the World Trade Organization Secretariat. It submits proposals for structural reform. It qualifies Panel reports as a product of a legal agency process in global administrative law. It consequently recalibrates the role of the Appellate Body with a view to bring about checks and balances. This paper explores the neglected role of standards of review of the Appellate Body vis-à-vis Panels. While securing a continued leading role of the Appellate Body on constitutional issues and fundamental principles of the system, it suggests a more deferential standard of reasonableness in reviewing Panel findings on World Trade Organization rules.
Enhancing the developing brain: tensions between parent, child, and state in the United States
Authors: Jwa Anita S 
Published: April 2021

ABSTRACT
Recent technological advances in neuroscience offer a novel way for parents to nurture their children: altering brain activation to improve cognitive functions. Parental use and state regulation of cognitive enhancement will inevitably cause tensions between parent, child, and state. These tensions stem from three different but fundamentally related causes, namely minors’ incompetency in making decisions about their own welfare, parental autonomy to make decisions about the upbringing of their minor children, and the state’s interests in protecting minors’ well-being. However, these tensions are not without precedents. The courts have frequently struggled to set the boundary of parental autonomy and to balance parents’ rights, children’s interests, and state’s interests, and have accumulated extensive precedents in various contexts. This article reviews previous US court decisions in select contexts analogous to cognitive enhancement—medical intervention, education, and mandatory vaccination—and analyzes their implications for the use of cognitive enhancement on minors. This article will provide a useful guide for policy makers and researchers to identify and analyze issues regarding cognitive enhancement and to develop sound policies to ensure responsible use of this novel technology.
Screening for Problematic Sleep in a Diverse Sample of Infants
Authors: Honaker Sarah M , McQuillan Maureen E , Mindell Jodi A , Downs Stephen M , Slaven James E , Schwichtenberg A J 
Published: April 2021

AbstractObjectiveTo examine screening strategies for identifying problematic sleep in a diverse sample of infants.MethodsParents of infants (5–19 months; N = 3,271) presenting for a primary care visit responded to five screening items and the Infant Sleep Questionnaire (ISQ), a validated measure of problematic infant sleep. If parents responded affirmatively to any screening item, primary care providers received a prompt to evaluate. For each of the screening questions, we examined differences in item endorsement and criterion related validity with the ISQ. Using conceptual composites of night waking and sleep difficulty, prevalence, criterion-related validity, and concurrent demographic correlates were analyzed.ResultsInfants were primarily of Black race (50.1%) or Hispanic ethnicity (31.7%), with the majority (63.3%) living in economically distressed communities. Rates of problematic sleep ranged from 7.4%, for a single item assessing parental perception of an infant having a sleep problem, to 74.0%, for a single item assessing night wakings requiring adult intervention. Items assessing sleep difficulty had high (95.0–97.8%) agreement with the ISQ in identifying infants without problematic sleep, but low agreement (24.9–34.0%) in identifying those with problematic sleep. The opposite was true for items assessing night waking, which identified 91.0–94.6% of those with sleep problems but only 31.8–46.9% of those without.ConclusionsScreening strategies for identifying problematic infant sleep yielded highly variable prevalence rates and associated factors, depending on whether the strategy emphasized parent-perceived sleep difficulty or night wakings. The strategy that is most appropriate will depend on the system’s goals.