bluuebunny/crossref_metadata_2025_split huggingface dataset:
Bone conducted responses in the neonatal rat auditory cortex
Authors: Makarov Roman , Sintsov Mikhail , Valeeva Guzel , Starikov Pavel , Negrov Dmitriy , Khazipov Roustem 
Published: August 2021

AbstractRats are born deaf and start hearing at the end of the second postnatal week, when the ear canals open and low-intensity sounds start to evoke responses in the auditory cortex. Here, using μECoG electrode arrays and intracortical silicon probe recordings, we found that bone-conducted (BC) sounds evoked biphasic responses in the auditory cortex starting from postnatal day (P) 8. The initial phase of these responses, generated by thalamocortical input, was followed by intracortical propagation within supragranular layers. BC-evoked responses co-localized with the responses evoked by electrical stimulation of the cochlea and the deepest layers of the inferior colliculus prior to onset of low-threshold hearing (P13), as well as with the responses evoked by high-frequency (30 kHz) low-intensity (70 dB) air-conducted sounds after that. Thus, BC signals reach high-frequency processing regions of the auditory cortex well before the onset of low-threshold hearing, reflecting early integrity of the auditory system.
Grazing intensity drives plant diversity but does not affect forage production in a natural grassland dominated by the tussock-forming grass Andropogon lateralis Nees
Authors: Zanella Pablo Giliard , Junior Luis Henrique Paim Della Giustina , Pinto Cassiano Eduardo , Baldissera Tiago Celso , Werner Simone Silmara , Garagorry Fabio Cervo , Jaurena Martín , Lattanzi Fernando Alfredo , Sbrissia André Fischer 
Published: August 2021

AbstractAndropogon lateralis is a tall and highly plastic tussock-forming grass native from southern South America. It is a frequent component of Campos and Subtropical highland grasslands that often becomes dominant under lax grazing regimes. The aim of this work was to analyze the response of species diversity and forage production of a natural grassland dominated by A. lateralis to a wide range of grazing intensity. We hypothesized that species diversity and forage production would both peak at the intermediate canopy heights determined by grazing regimes of moderate intensity. A grazing experiment was conducted in a highland grassland with mesothermal humid climate at 922 masl (Atlantic Forest biome, Santa Catarina state, Brazil) that comprised 87 species from 20 families but had 50% of its standing biomass accounted by A. lateralis. Four pre-/post-grazing canopy heights—12/7, 20/12, 28/17, and 36/22 cm (measured on A. lateralis)—were arranged in a complete randomized block design with four replications, and intermittently stocked with beef heifers from October 2015 to October 2017. Andropogon lateralis cover decreased (from 75 to 50%), and species richness increased (15–25 species m−2) as canopy height decreased. Grazing intensity did not affect annual forage production (4.2 Mg DM ha−1). This natural grassland dominated by A. lateralis had a high capacity to adjust to grazing regimes of contrasting intensity, maintaining forage production stable over a wide range of canopy heights. However, to prevent losses in floristic diversity, such grassland should not be grazed at canopy heights higher than 28 cm.
Amazon rainforest rodents (Proechimys) are resistant to post-stroke epilepsy
Authors: Ortiz-Villatoro Nancy N. , Reyes-Garcia Selvin Z. , Freitas Leandro , Rodrigues Laís D. , Santos Luiz E. C. , Faber Jean , Cavalheiro Esper A. , Finsterer Josef , Scorza Fulvio A. , de Almeida Antônio C. G. , Scorza Carla A. 
Published: August 2021

AbstractThere are no clinical interventions to prevent post-injury epilepsy, a common and devastating outcome after brain insults. Epileptogenic events that run from brain injury to epilepsy are poorly understood. Previous studies in our laboratory suggested Proechimys, an exotic Amazonian rodent, as resistant to acquired epilepsy development in post-status epilepticus models. The present comparative study was conducted to assess (1) stroke-related brain responses 24-h and 30 days after cortical photothrombosis and (2) post-stroke epilepsy between Proechimys rodents and Wistar rats, a traditional animal used for laboratory research. Proechimys group showed smaller volume of ischemic infarction and lesser glial activation than Wistar group. In contrast to Wistar rats, post-stroke decreased levels of pro-inflammatory cytokines and increased levels of anti-inflammatory mediators and growth factors were found in Proechimys. Electrophysiological signaling changes assessed by cortical spreading depression, in vitro and in vivo, showed that Wistar’s brain is most severely affected by stroke. Chronic electrocorticographic recordings showed that injury did not lead to epilepsy in Proechimys whereas 88% of the Wistar rats developed post-stroke epilepsy. Science gains insights from comparative studies on diverse species. Proechimys rodents proved to be a useful animal model to study antiepileptogenic mechanisms after brain insults and complement conventional animal models.
Inverse association between use of broad spectrum penicllin with beta-lactamase inhibitors and prevalence of type 1 diabetes mellitus in Europe
Authors: Ternák Gábor , Berényi Károly , Kun Szilárd , Szigeti Nóra , Decsi Tamás , Sütő Gábor , Wittmann István 
Published: August 2021

AbstractIncreasing incidence of type 1 diabetes is supposed to be induced by environmental factors. Microbiome modulated by antibiotics seems to serve as one of the environmental factors which could influence the development of T1DM. Mitochondria, as autochthonous environmental bacteria living in our cells, and other bacteria share many common enzymes including beta-lactamases and it is supported by evidence that some beta-lactamase inhibitors are able to interact with counterpart enzymes. Thus, antibiotics may utilize two different pathways influencing the development of T1DM; one through modulation of microbiome and a second one via the interaction of mitochondrial enzymes. Data of consumption of penicillin (both narrow and broad spectrum) and beta-lactamase inhibitors in 30 European countries were collected from the database of the European Centre for Disease Prevention and Control. These data were correlated with the prevalence reported by the International Diabetes Federation (2019) referring to type 1 diabetes in Europe. No correlation was found between total penicillin consumption or use of broad spectrum penicillin and the prevalence of type 1 diabetes. Nevertheless, broad spectrum penicillin, in combination with beta-lactamase inhibitor, was in inverse correlation with the prevalence of type 1 diabetes (r = − 0.573, p = 0.001). On the other hand, narrow spectrum penicillin was in positive correlation with type 1 diabetes (r = 0.523, p = 0.003). Prevalence of type 1 diabetes showed an inverse correlation with the use of beta-lactamase inhibitors and a positive one with that of narrow spectrum penicillin. Such a detailed analysis has not so far been provided referring to the penicillin group. In the background of this association either microbiomal or direct mitochondrial effects can be supposed.
Influence of variable biochar concentration on yield-scaled nitrous oxide emissions, Wheat yield and nitrogen use efficiency
Authors: Dawar Khadim , Saif-ur-Rahman  , Fahad Shah , Alam Syed Sartaj , Khan Shah Alam , Dawar Atif , Younis Uzma , Danish Subhan , Datta Rahul , Dick Richard P. 
Published: August 2021

AbstractAn important source of the destructive greenhouse gas, nitrous oxide (N2O) comes from the use of ammonium based nitrogen (N) fertilizers that release N2O in the incomplete conversion (nitrification) of NH4+ to NO3ˉ1. Biochar has been shown to decrease nitrification rates and N2O emission. However, there is little information from semi-arid environments such as in Pakistan where conditions favor N2O emissions. Therefore, the object was to conduct field experiment to determine the impact of biochar rates in the presence or absence of urea amended soils on yield-scaled N2O emissions, and wheat yield and N use efficiency (NUE). The experiment on wheat (Triticum aestivumL.), had a randomized complete block design with four replications and the treatments: control, sole urea (150 kg N ha−1), 5 Mg biochar ha−1(B5), 10 Mg biochar ha−1(B10), urea + B5 or urea + B10. In urea amended soils with B5 or B10 treatments, biochar reduced total N2O emissions by 27 and 35%, respectively, over the sole urea treatment. Urea + B5 or + B10 treatments had 34 and 46% lower levels, respectively, of yield scaled N2O over the sole urea treatment. The B5 and B10 treatments had 24–38%, 9–13%, 12–27% and 35–43%, respectively greater wheat above-ground biomass, grain yield, total N uptake, and NUE, over sole urea. The biochar treatments increased the retention of NH4+ which likely was an important mechanism for reducing N2O by limiting nitrification. These results indicate that amending soils with biochar has potential to mitigate N2O emissions in a semi-arid and at the same time increase wheat productivity.
Exposure to foreign gut microbiota can facilitate rapid dietary shifts
Authors: Heys C. , Fisher A. M. , Dewhurst A. D. , Lewis Z. , Lizé A. 
Published: August 2021

AbstractDietary niche is fundamental for determining species ecology; thus, a detailed understanding of what drives variation in dietary niche is vital for predicting ecological shifts and could have implications for species management. Gut microbiota can be important for determining an organism’s dietary preference, and therefore which food resources they are likely to exploit. Evidence for whether the composition of the gut microbiota is plastic in response to changes in diet is mixed. Also, the extent to which dietary preference can be changed following colonisation by new gut microbiota from different species is unknown. Here, we use Drosophila spp. to show that: (1) the composition of an individual’s gut microbiota can change in response to dietary changes, and (2) ingestion of foreign gut microbes can cause individuals to be attracted to food types they previously had a strong aversion to. Thus, we expose a mechanism for facilitating rapid shifts in dietary niche over short evolutionary timescales.
Astrocyte-to-neuron transportation of enhanced green fluorescent protein in cerebral cortex requires F-actin dependent tunneling nanotubes
Authors: Chen Jing , Cao Junyan 
Published: August 2021

AbstractTunneling nanotube (TNT), a dynamic cell–cell contact, is dependent on actin polymerization. TNTs are efficient in transporting ions, proteins and organelles intercellularly, which are important mechanisms in physiological and pathological processes. Reported studies on the existence and function of TNTs among neural cells focus on cultured cell for the convenience in detecting TNTs’ ultrastructure. In this study, the adeno-associated virus (AAV-GFAP-EGFP-p2A-cre) was injected into the cerebral cortex of knock-in mice ROSA26 GNZ. GFAP promoter initiated the expression of enhanced green fluorescent protein (EGFP) in infected astrocytes. At 10 days post injection (10 DPI), EGFP transferred from astrocytes in layer I–III to neurons in layer V. The dissemination of EGFP was not through endocytosis or exosome. Applying microscopes, we found that the intercellular transportation of EGFP through contact connection was F-actin dependent. Therefore, we concluded that EGFP transported from astrocytes to neurons in cortex via F-actin dependent TNTs. This study first proved that proteins transported intercellularly via TNTs in brain.
Survival analysis in breast cancer using proteomic data from four independent datasets
Authors: Ősz Ágnes , Lánczky András , Győrffy Balázs 
Published: August 2021

AbstractBreast cancer clinical treatment selection is based on the immunohistochemical determination of four protein biomarkers: ESR1, PGR, HER2, and MKI67. Our aim was to correlate immunohistochemical results to proteome-level technologies in measuring the expression of these markers. We also aimed to integrate available proteome-level breast cancer datasets to identify and validate new prognostic biomarker candidates. We searched studies involving breast cancer patient cohorts with published survival and proteomic information. Immunohistochemistry and proteomic technologies were compared using the Mann–Whitney test. Receiver operating characteristics (ROC) curves were generated to validate discriminative power. Cox regression and Kaplan–Meier survival analysis were calculated to assess prognostic power. False Discovery Rate was computed to correct for multiple hypothesis testing. We established a database integrating protein expression data and survival information from four independent cohorts for 1229 breast cancer patients. In all four studies combined, a total of 7342 unique proteins were identified, and 1417 of these were identified in at least three datasets. ESR1, PGR, and HER2 protein expression levels determined by RPPA or LC–MS/MS methods showed a significant correlation with the levels determined by immunohistochemistry (p < 0.0001). PGR and ESR1 levels showed a moderate correlation (correlation coefficient = 0.17, p = 0.0399). An additional panel of candidate proteins, including apoptosis-related proteins (BCL2,), adhesion markers (CDH1, CLDN3, CLDN7) and basal markers (cytokeratins), were validated as prognostic biomarkers. Finally, we expanded our previously established web tool designed to validate survival-associated biomarkers by including the proteomic datasets analyzed in this study (https://kmplot.com/). In summary, large proteomic studies now provide sufficient data enabling the validation and ranking of potential protein biomarkers.
Insolation-paced sea level and sediment flux during the early Pleistocene in Southeast Asia
Authors: Vaucher Romain , Dashtgard Shahin E. , Horng Chorng-Shern , Zeeden Christian , Dillinger Antoine , Pan Yu-Yen , Setiaji Romy A. , Chi Wen-Rong , Löwemark Ludvig 
Published: August 2021

AbstractGlobal marine archives from the early Pleistocene indicate that glacial-interglacial cycles, and their corresponding sea-level cycles, have predominantly a periodicity of ~ 41 kyrs driven by Earth’s obliquity. Here, we present a clastic shallow-marine record from the early Pleistocene in Southeast Asia (Cholan Formation, Taiwan). The studied strata comprise stacked cyclic successions deposited in offshore to nearshore environments in the paleo-Taiwan Strait. The stratigraphy was compared to both a δ18O isotope record of benthic foraminifera and orbital parameters driving insolation at the time of deposition. Analyses indicate a strong correlation between depositional cycles and Northern Hemisphere summer insolation, which is precession-dominated with an obliquity component. Our results represent geological evidence of precession-dominated sea-level fluctuations during the early Pleistocene, independent of a global ice-volume proxy. Preservation of this signal is possible due to the high-accommodation creation and high-sedimentation rate in the basin enhancing the completeness of the stratigraphic record.
Modeling the effects of EMT-immune dynamics on carcinoma disease progression
Authors: Bergman Daniel R. , Karikomi Matthew K. , Yu Min , Nie Qing , MacLean Adam L. 
Published: August 2021

AbstractDuring progression from carcinoma in situ to an invasive tumor, the immune system is engaged in complex sets of interactions with various tumor cells. Tumor cell plasticity alters disease trajectories via epithelial-to-mesenchymal transition (EMT). Several of the same pathways that regulate EMT are involved in tumor-immune interactions, yet little is known about the mechanisms and consequences of crosstalk between these regulatory processes. Here we introduce a multiscale evolutionary model to describe tumor-immune-EMT interactions and their impact on epithelial cancer progression from in situ to invasive disease. Through simulation of patient cohorts in silico, the model predicts that a controllable region maximizes invasion-free survival. This controllable region depends on properties of the mesenchymal tumor cell phenotype: its growth rate and its immune-evasiveness. In light of the model predictions, we analyze EMT-inflammation-associated data from The Cancer Genome Atlas, and find that association with EMT worsens invasion-free survival probabilities. This result supports the predictions of the model, and leads to the identification of genes that influence outcomes in bladder and uterine cancer, including FGF pathway members. These results suggest new means to delay disease progression, and demonstrate the importance of studying cancer-immune interactions in light of EMT.
High geothermal heat flow beneath Thwaites Glacier in West Antarctica inferred from aeromagnetic data
Authors: Dziadek Ricarda , Ferraccioli Fausto , Gohl Karsten 
Published: August 2021

AbstractGeothermal heat flow in the polar regions plays a crucial role in understanding ice-sheet dynamics and predictions of sea level rise. Continental-scale indirect estimates often have a low spatial resolution and yield largest discrepancies in West Antarctica. Here we analyse geophysical data to estimate geothermal heat flow in the Amundsen Sea Sector of West Antarctica. With Curie depth analysis based on a new magnetic anomaly grid compilation, we reveal variations in lithospheric thermal gradients. We show that the rapidly retreating Thwaites and Pope glaciers in particular are underlain by areas of largely elevated geothermal heat flow, which relates to the tectonic and magmatic history of the West Antarctic Rift System in this region. Our results imply that the behavior of this vulnerable sector of the West Antarctic Ice Sheet is strongly coupled to the dynamics of the underlying lithosphere.
Refeeding-Syndrom: Was wissen Humanmedizinstudierende im letzten Studiensemester?
Authors: Bauer Mirjam , Dolder Andrea , Stanga Zeno , Kurmann Silvia 
Published: August 2021

Zusammenfassung
Hintergrund Das Refeeding-Syndrom (RFS) ist eine Stoffwechselreaktion verursacht durch eine inadäquate Wiederernährung nach einer Hungerphase. Das RFS ist gekennzeichnet durch eine Serumelektrolyt- und Flüssigkeitsverschiebung, welche schwerwiegende Folgen wie Herzrhythmusstörungen verursachen können und gelegentlich zum Tod führen. Zur Prävention sind eine frühzeitige Risikoerkennung sowie ein angepasstes Ernährungsmanagement notwendig. Verschiedene Studien weisen auf ein unzureichendes Wissen der Ärzteschaft hin. Für die Schweiz existieren zum heutigen Zeitpunkt keine Daten. Mit dieser Studie wurde der Wissensstand der Humanmedizinstudierenden der Deutschschweiz im letzten Semester zum RFS untersucht.
Material und Methodik Das Wissen wurde mittels Onlinefragebogen erfasst. Dieser enthielt 15 Multiple-Choice-Fragen. Die maximal mögliche Gesamtpunktzahl betrug 20 Punkte. Die Ergebnisse wurden gemäß Schweizer Notenberechnung beurteilt (beste Note 6, schlechteste Note 1). 60 % der Punkte mussten erreicht werden, um ein genügendes Ergebnis (Note 4) zu erreichen.
Ergebnisse 79 von 540 Studierenden beantworteten den Fragebogen, was einer Rücklaufquote von 14,6 % entspricht. Die Studierenden erreichten durchschnittlich 9,9 Punkte (50 % der Gesamtpunktzahl). Das Resultat von 53 % der Studierenden war ungenügend. Neun von 79 Studierenden (11,4 %) war das RFS unbekannt.
Schlussfolgerung Das Wissen der Schweizer Humanmedizinstudierenden zum RFS ist nicht zufriedenstellend und sollte unbedingt optimiert werden. In stationären Einrichtungen ist ein flächendeckendes Ernährungsscreening mit einem validierten Instrument empfohlen. Risikopatienten für eine Mangelernährung sollten an eine Ernährungsberatung (ERB) überwiesen werden. Die ERB erkennt im Ernährungsassessment das Risiko für ein RFS und kann in Zusammenarbeit mit der behandelnden Ärzteschaft den Ernährungsaufbau planen. Eine gute multiprofessionelle Zusammenarbeit ist unerlässlich, um Komplikationen zu verhindern.
Maßnahmen zur Förderung der kardiovaskulären Gesundheit: Kardioprotektive Lebensmittel und die Nationale Reduktions- und Innovationsstrategie
Authors: Breneise Regine , Weinberger Natascha-Alexandra , Lehne Christine , Wiacek Claudia , Braun Peggy , Luck-Sikorski Claudia 
Published: August 2021

Zusammenfassung
Einleitung Eine ausgewogene und herzgesündere Ernährung kann vorzeitigen Todesfällen durch Herz-Kreislauf-Erkrankungen präventiv entgegenwirken. Die Studie untersucht den derzeitigen Wissensstand der Bevölkerung zu ernährungsrelevanten Maßnahmen zur Förderung von kardiovaskulärer Gesundheit. Dabei wird auf Lebensmittel mit zusätzlicher kardioprotektiver Wirkung und regierungsgesteuerten Maßnahmen wie der Nationalen Reduktions- und Innovationsstrategie eingegangen.
Methode Es wurden 1003 Personen telefonisch zu den Themen kardioprotektive Lebensmittel und Nationale Reduktions- und Innovationsstrategie befragt.
Ergebnisse Die Ergebnisse zeigen, dass ein geringer Teil der Befragten (17,3 %) wusste, was Lebensmittel mit kardioprotektiver Wirkung sind. Außerdem herrschte auch beim Großteil der Befragten (81,6 %) Unwissenheit gegenüber der Nationalen Reduktions- und Innovationsstrategie.
Fazit Das Wissen über unterschiedliche kardiovaskulär ernährungsrelevante Maßnahmen könnte die Verbraucherakzeptanz erhöhen und langfristig zu einer verringerten Anzahl an Herz-Kreislauferkrankungen durch Ernährungsrisiken führen.
Association of Serum Myostatin with Body Weight, Visceral Fat Volume, and High Sensitivity C-Reactive Protein But Not With Muscle Mass and Physical Fitness in Premenopausal Women
Authors: Kern-Matschilles Stefanie , Gar Christina , Wanger Lorena , Haschka Stefanie J. , Potzel Anne L. , Hesse Nina , Then Cornelia , Seissler Jochen , Lechner Andreas 
Published: August 2021

Abstract
Background The myokine myostatin regulates muscle mass and has been linked to insulin resistance and metabolic syndrome. However, data on its role in humans is still limited. We, therefore, investigated the associations of serum myostatin with muscle mass, physical fitness, and components of the metabolic syndrome in a cohort of premenopausal women.
Methods We undertook a cross-sectional analysis of 233 women from the monocenter study PPSDiab, conducted in Munich, Germany. Participants had recently completed a pregnancy with or without gestational diabetes. Our analysis included medical history, anthropometrics, oral glucose tolerance testing, laboratory chemistry, cardiopulmonary exercise testing, and magnetic resonance imaging (n=142) of visceral fat volume, left quadriceps muscle mass, and muscle fat content. Serum myostatin was quantified by a competitive enzyme-linked immunosorbent assay.
Results We observed positive correlations of serum myostatin with body mass index (ρ=0.235; p=0.0003), body fat percentage (ρ=0.166; p=0.011), waist circumference (ρ=0.206; p=0.002), intraabdominal fat volume (ρ=0.182; p=0.030) and high-sensitivity C-reactive protein (ρ=0.175; p=0.008). These correlations were reproduced in linear regression analyses with adjustment for age and time after delivery. We saw no correlations with muscle mass, physical fitness, insulin sensitivity, triglycerides, HDL cholesterol, and blood pressure.
Conclusions Our observation of elevated serum myostatin in women with a higher body fat percentage, visceral obesity, and elevated c-reactive protein suggests that this myokine contributes to the altered muscle-adipose tissue crosstalk in metabolic syndrome. Elevated myostatin may advance this pathophysiologic process and could also impair the efficacy of exercise interventions. Further mechanistic studies, therefore, seem warranted.