bluuebunny/crossref_metadata_2025_split huggingface dataset:
Silencing miR-199a-3p, Which Targets Integrin Β8 Prevents Steroid-induced Avascular Necrosis of the Femoral Head by Regulating Osteogenesis and Adipogenesis
Authors: Yang Wu , Yang Yunfei , Guo Minkang , Zhu Weiwen , Qian Husun , Lian Chengjie , Bai Haobo , Wang Hongwei , Chen Tingmei , zhang jian 
Published: July 2021

Abstract
BackgroundThe main pathogenesis of steroid-induced avascular necrosis of the femoral head (SANFH) is closely connected with osteogenesis and adipogenesis. MicroRNAs have been proved to play prominent roles in the initiation and progression of SANFH. The present study investigated the effects of silencing miR-199a-3p on the prevention and early treatment of SANFH. MethodsRT-PCR was used to detect the expression of miR-199a-3p. Western blotting was used to detect protein expression. Alkaline phosphatase (ALP) activity, alizarin red S staining, and oil red O staining were used to study the effect of miR-199a-3p on the osteogenic and adipogenic differentiation of rat bone marrow-derived mesenchymal stem cells (rBMSCs) and MC3T3-E1 cells. A dual-luciferase reporter assay was used to confirm the target relation of miR-199a-3p and integrin β8 (ITGB8). Animal study was used to explore the effect of silencing miR-199a-3p in vivo.ResultsMiR-199a-3p expression was upregulated in necrotic bone tissues and rBMSCs and MC3T3-E1 cells treated with 20 UM dexamethasone. Western blotting and a dual luciferase assay were employed to verify that miR-199a-3p directly targets ITGB8. Silencing of miR-199a-3p also promoted osteogenesis and inhibited adipogenesis of rBMSCs and MC3T3-E1 cells in the presence or absence of glucocorticoids. In addition, the silence of miR-199A-3p prevent SANFH by promoting osteogenesis in rats.ConclusionThe upregulation of miR-199a-3p induced by GCs could reciprocally regulate the adipo-osteogenic differentiation of rBMSCs and MC3T3-E1 cells by targeting ITGB8. The miR-199a-3p-ITGB8-FAK-Erk1/2-RUNX2 axis is a potential signaling mechanism, and inhibition of miR-199a-3p expression could promote osteogenesis and inhibit adipogenesis to alleviate the occurrence of SANFH.
Numerical Treatment for the Second Law Analysis in Hydromagnetic Peristaltic Nanomaterial Rheology: Endoscopy Applications
Authors: Awais Muhammad , Shoaib Muhammad , Raja Muhammad Asif Zahoor , Arif Saba , Malik Muhammad Yousaf , Nisar Kottakkaran Sooppy 
Published: July 2021

Abstract
In current study, analysis is presented for peristaltic motion of applied magnetic field and entropy generation within couple stress (Cu/H2O) nanofluid through an endoscope. An endoscope contains two coaxial cylindrical tubes in which the internal tube is nonflexible while the external tube has sinusoidal wave passing through the boundary. Influences of mixed convection along with applied magnetic field are encountered as well. Formulated governing model is fabricated introducing long wavelength and creeping Stokesian flow approximation which are then analyzed numerically by utilizing Adams Bashforth method. For a physical insight, results are demonstrated to examine the behaviors of flow profiles and entropy generation number for emerging flow parameters with the help of graphs, bar-charts and tables.
Plant Based High Energy Mash Diets Supplemented With Nahco3, L-arginine+ Vitamin-c and Vegetable Oils Are Effective Against Tachycardia and Polycythemia in Broiler Chicken
Authors: Hossain Md Emran , Akter Nasima 
Published: July 2021

Abstract
The study aimed to investigate if plant based high energy mash diets supplemented with NaHCO3, L-arginine + vitamin-C and vegetable oils were effective against tachycardia and polycythemia in the commercial broiler chicken. Total 256Ross-308 day old male broiler chicks were randomly distributed into eight dietary treatment groups in a three way 23 factorial arrangements (Three factors, i.e., NaHCO3, L-arginine + vitamin-C and vegetable oil each with two levels, e.g., 0 and 0.1% for NaHCO3 and L-arginine + vitamin-C; 3 and 4% of vegetable oil supplemented with basal diet). Iso-caloric and iso-nitrogenous diets were formulated and supplied ad libitum. The average daily feed intake (ADFI), average daily gain (ADG), feed efficiency (FE), carcass characteristics, cardio-pulmonary morphometry, total protein (TP), hemoglobin (Hb), triiodothyronine (T3), incidence of tachycardia and polycythemia were examined up to 35 d. Supplementation of NaHCO3decreased (p<0.001) the ADFI at 1-14 d, 15-35 d, 1-35 d, improved (p<0.01) the FE at 1-14 d and increased (p<0.05) the serum TP. Dietary L-arginine + vitamin-C decreased (p<0.01) the heart rate without affecting the performance parameters, carcass characteristics and hemato-biochemical indices. Supplementation of vegetable oil decreased (p<0.01) the ADFI at 1-14d, 15-35 d, 1-35 d, increased (p<0.01) the ADG at 1-14d, improved (p<0.001) the FE at 1-14 d and increased (p<0.05) the heart rate, Hb and PCV. Further, NaHCO3, L-arginine + vitamin-C and vegetable oil synergistically interacted to decrease the left and right ventricular weight, RV:TV and increased the T3 without affecting overall performance, carcass characteristics and hemato-biochemical indices. It was concluded that, plant based high energy mash diets are not susceptible to tachycardia and polycythemia. Addition of NaHCO3, L-arginine + vitamin-C ameliorate the propensity of tachycardia and polycythemia without deteriorating performance, carcass characteristics and hemato-biochemical indices of the commercial broiler birds in a dose dependent manner.
Geometric Morphometrics Captures Variations in Wing Development of Black Soldier Flies
Authors: Gyimah Obediah , Combey Rofela , Ewusie Ebenezer Ato , Badu Isaac , Yeboah Simon , Apaatah Francis , Nagetey Amuetey , Quandahor Peter 
Published: July 2021

Abstract
The black soldier fly is considered an important insect due to its ability to convert organic wast into high quality organic fertilizer. Studies have shown that food quality at the larvae stage contributes greatly to their development. In this study, we applied geometric morphometric measurements to access the variations in the black soldier fly fed on different food substrates as captured in the wings. Eggs of black soldier fly were collected from the field and cultured on different food substrates such as; restaurant waste, fruit waste, wheat brand and layer meal until maturity. The right fore wing of 140 individuals of black were used for the experiment. The results showed a significant difference between the shape of landmarks within each food substrate group. Landmark 3, 7 and 9, corresponding to end of first radial vein R1, intersession between medio-cubital vein and cubito-anal vein (m-cu and CuA), and intersission between cubito Anal vein and Anal vein (CuA + Cup) contributed most to the variation between the different food substrates. Partial least square showed a strong association between food substrate and wing development. Therefore, the variations in wing development could be due to the impact of nutritional compositions quality of the different food substrates.
Assessment of α-cypermethrin Pour-on Application and Diminazene Aceturate for the Treatment of Trypanosome-related Diseases Caused by Tsetse Flies in Cattle in Mô (Togo)
Authors: Boma Soudah , Talaki Essodina , N'Féidé Toï , Dao Balabadi , Lombo Yao , Somda Martin Bienvenu , Salou Wendemanegdé Ernest 
Published: July 2021

Abstract
BackgroundThe effects of tsetse-transmitted trypanosomosis control in high tsetse challenge and trypanocidal drug resistance settings remain poorly understood in Togo, owing to the poor data underlying the current disease impact. This study was implemented in the framework of the PDRI-Mô project which is an African animal trypanosomosis control project using trypanocides and insecticides on cattle. This project, planned by the agricultural ministry, focused on all the sedentary cattle breeds in the 1000 km² area of the prefecture of Mô. Until 2013, the locality remained unconnected to outside areas, with long-term limited access to quality medicines, veterinary services, animal husbandry and new AAT control technologies. Methods From March 2014 to November 2017, a database of zoo-sanitary surveys integrating the evolution of the incidence of the disease and the coverage of interventions made it possible to quantify the apparent effect attributable to control effort in herds. The strategy involved an initial phase with cross-sectional entomological and parasitological surveys, including a rapid trypanocidal drug sensitivity testing. Treatment efficacy was assessed using parasitological status post-treatment of trypanosome-positive animals in each village, randomly assigned to three groups, one treated with 0.5 mg/kg b.w. Isometamidium chloride, the second with 3.5 mg/kg b.w. Diminazene diaceturate and the third with distillated water as control. Using parasitological status of blood sample collected on day 0, day 14 and day 28 post-treatment as the outcome result, trypanosome phenotype resistance of drugs treatment was determined if relapse occur. Then, three times a year, 20% of the herd received α-cypermethrin pour-on, and parasitaemic cattle with poor health were individually given diminazene aceturate at 7 mg/kg of body weight (b.w.).ResultsThe tsetse density in the area decreased significantly (P-value ˂ 0.001) from 1.78 ± 0.37 in March 2014 prior to the α-cypermethrin application to 0.48 ± 0.07 in February 2017. Prior to the trypanocidal treatment, relapse of trypanosome infections in the cattle was approximately 12.5% for diminazene at 3.5 mg/kg of b.w., 35% for isometamidium at 0.5 mg/kg of b.w. and null for diminazeen at 7 mg/kg of b.w. Target deployment led to the largest reduction in disease incidence from 28.1% in 2014 to 7.8% in 2017, an improvement in haematocrit from 24.27±4.9% to 27.5±4.6% and a reduction in calf mortality from 15.9±11% to 5.9%.ConclusionsImproving the access to these interventions for different types of livestock and maintaining their effectiveness in the face of high tsetse challenges should be the primary focus of control strategies in areas of Togo.
Evaluating factors that influenced the successful implementation of an evidence-based neonatal care intervention in Chinese hospitals using the PARIHS framework
Authors: Yue Jieya , Liu Jun , Zhao Yingxi , Williams Sarah , Zhang Bo , Zhang Lin , Zhang Qiannan , Liu Xin , Wall Stephen , Zhao Gengli 
Published: July 2021

Abstract
Background
Evidence based interventions (EBIs) can improve patient care and outcomes. Understanding the process for successfully introducing and implementing EBIs can inform effective roll-out and scale up. The Promoting Action on Research Implementation in Health Services (PARIHS) framework can be used to evaluate and guide the introduction and implementation of EBIs. To gain an understanding of the utility of the PARIHS framework in China and investigate how important each of its constructs (evidence, context and facilitation) and sub-elements are perceived to be to successful implementation of EBIs in a Chinese setting, we used the framework to assess the implementation of an evidence-based neonatal intervention (kangaroo mother care, KMC) recently introduced in selected Chinese hospitals.
Method
We conducted clinical observations and semi-structured interviews with 10 physicians and 18 nurses in five tertiary hospitals implementing KMC. Interview questions were organized around issues including knowledge and beliefs, resources, culture, implementation readiness and climate. We used directed content analysis to analyze the interview transcript, amending the PARIHS framework to incorporate emerging sub-themes. We also rated the constructs and sub-elements on a continuum from “low (weak)”, “moderate” or “high (strong)” highlighting the ones considered most influential for hospital level implementation by study participants.
Results
Our finding suggest that clinical experience, culture, leadership, evaluation, and facilitation are highly influential sub-elements for EBI implementation in China. External evidence had a moderate impact, especially in the initial awareness raising stages of implementation and resources were also considered to be of moderate importance, although this may change as implementation progresses. Patient experience was not seen as a driver for implementation at hospital level.
Conclusion
The PHARIS framework can be a useful tool for planning and evaluating EBI implementation in China. However, it’s sub-elements should be assessed and adapted to the implementation setting.
Evaluation of saliva molecular point of care for detection of SARS-CoV-2 in ambulatory care
Authors: LeGoff Jérôme , Kernéis Solen , Elie Caroline , Delarue Séverine Mercier , Gastli Nabil , Choupeaux Laure , Fourgeaud Jacques , Alby Marie-Laure , Quentin Pierre , Pavie Juliette , Brazille Patricia , Néré Marie Laure , Minier Marine , Gabassi Audrey , Leroy Chrystel , Parfait Béatrice , Tréluyer Jean-Marc , Delaugerre Constance 
Published: July 2021

Abstract
Background
Rapid identification of SARS-Cov-2 infected individuals is a cornerstone for the control of virus spread. The sensitivity of SARS-CoV-2 RNA detection by RT-PCR is similar in saliva and nasopharyngeal swab. Rapid molecular point-of-care tests in saliva could facilitate, broaden and speed up the diagnosis.
Methods
We conducted a prospective study in two community COVID-19 screening centers to evaluate the performances of a CE-marked RT-LAMP assay (EasyCoV™) designed for the detection of SARS-CoV2 RNA from fresh saliva samples, compared to nasopharyngeal RT-PCR, to saliva RT-PCR and to nasopharyngeal antigen testing.
Results
Overall, 117 of the 1718 participants (7%) were tested positive with nasopharyngeal RT-PCR. Compared to nasopharyngeal RT-PCR, the sensitivity and specificity of the RT-LAMP assay in saliva were 34% and 97% respectively. The Ct values of nasopharyngeal RT-PCR were significantly lower in the 40 true positive subjects with saliva RT-LAMP (Ct 25.9) than in the 48 false negative subjects with saliva RT-LAMP (Ct 28.4) (p = 0.028). Considering six alternate criteria for reference test, including saliva RT-PCR and nasopharyngeal antigen, the sensitivity of saliva RT-LAMP ranged between 27 and 44%.
Conclusion
The detection of SARS-CoV-2 from crude saliva samples with a RT-LAMP assay had a lower sensitivity than nasopharyngeal RT-PCR, saliva RT-PCR and nasopharyngeal antigen testing.
Registration number
: NCT04578509
Polymeric nanoparticles improved Curcumin brain delivery and its therapeutic efficacy against intracerebral hemorrhage
Authors: Yang Cong , Han Meng-Meng , Li Ruo-Yu , Yang Yu-Na , Zhou Li-Gui , Duan Li-Ning , Su Shi-Yu , Li Min , Wang Qi , Chen Tong-Kai , Mo Yousheng 
Published: July 2021

Abstract
Background: Intracerebral hemorrhage (ICH) is a severe type of stroke. Ferroptosis is a new form of regulated cell death, which plays an indispensable role in the pathological process of ICH. Curcumin (Cur), a widespread phenolic compound, is derived from the rhizome of Curcuma longa. It could attenuate hematoma volume and neurological injury in ICH. Nevertheless, its poor solubility in water, low oral bioavailability and difficulty in transporting across physiological barriers led to poor efficacy. Polymer nanoparticles (NPs) are widely used drug delivery matrix material with good biocompatibility, which are reported to improve the bioavailability and pharmacokinetic profiles of drugs. In this study, we utilized NPs to encapsulate Cur and analyzed the effect of Cur-NPs on Cur brain delivery and its therapeutic efficacy against ICH. Results: The spherical Cur-NPs had a particle size 127.31±2.73 nm, a PDI of 0.21±0.01 and a zeta potential of -0.25±0.02 mV. Cur-NPs could draw into Madin-Darby canine kidney (MDCK) cells through a number of nonspecific endocytosis mechanisms, mainly mediated via clathrin and plasma membrane microcapsules. Moreover, Cur-NPs tended to accumulate in the endoplasmic reticulum and lysosome. In a zebrafish model, Cur-NPs could transport across physiological barriers. In a C57BL/6 mice model, we found that Cur-NPs had more desirable improvements in Cur accumulation within the plasma and brain. Importantly, in an ICH mouse model, we confirmed that Cur-NPs were an effective treatment for ICH. Finally, Cur-NPs effectively inhibited ferroptosis caused by erastin in HT22 mouse hippocampal cells. Conclusion: Cur-NPs represent a potentially effective strategy to enhance Cur brain delivery and therapeutic efficacy in the treatment of ICH.
Long Term Effects of Surgical And Transcatheter Aortic Valve Replacement On FFRCT In Patients With Severe Aortic Valve Stenosis.
Authors: Michiels Vincent , Andreini Daniele , Conte Edoardo , Tanaka Kaoru , Belsack Dries , Nijs Jan , Loo Ines Van , Argacha Jean Francois , Vandeloo Bert , Roosens Bram , Vandenbussche Karen , Cosyns Bernard 
Published: July 2021

Abstract
Background: the long-term variations of fractional flow reserve derived from coronary computed tomography (FFR CT ) after surgical (SAVR) or transcatheter (TAVR) aortic valve replacement in patients with severe aortic valve stenosis (AS) have not been investigated. Methods and Results: a total of 25 patients with isolated, severe AS underwent coronary computed tomography with 3-vessel FFR CT analysis (Heartflow Inc. - Redwood City, California, USA) and measurement of total coronary volume (V), left ventricular mass (M) and their ratio (V/M) before and 6 months after SAVR or TAVR. A significant increase in V/M due to a decrease in left ventricular mass 6 months after intervention was observed, whereas total coronary volume did not change (coronary volume pre: 2924,5 ± 867,9 mm 3 , coronary volume post: 2844,2 ± 792,8 mm 3 , P =0.158; LV mass pre: 151.7 ± 40.7 g, LV mass post: 127.3 ± 34.7 g, P <0.001; V/M pre: 19.5 ± 4.1 mm 3 /g, V/M post: 22.7 ± 4.28 mm 3 /g, P =0.002). FFR CT (expressed as area under the virtual pullback curve) remained constant. Conclusion: this proof-of-concept study showed that FFR CT was not subject to the confounding effect of left ventricular mass regression after SAVR or TAVR. Despite significant left ventricular remodeling at 6 months after AS treatment, FFR CT values remained constant. This means FFR CT can probably be used as a reliable test in AS patients but further studies are needed comparing the performance of the different invasive and non-invasive coronary physiological indices in this patient cohort.
An Improved Cutting Force Model in Micro-milling Considering the Comprehensive Effect of Tool Runout, Size Effect and Tool Wear
Authors: Liu Tongshun , Liu Yayun , Zhang Kedong 
Published: July 2021

Abstract
Tool runout, cutting edge radius-size effect and tool wear have significant impacts on the cutting force of micro-milling. In order to predict the micro-milling force and the machining performance related to the cutting force, it is necessary to establish a cutting force model including tool runout, cutting edge radius and tool wear. In this study, an instantaneous uncut thickness (IUCT) model considering tool runout, a nonlinear shear/ploughing coefficient model including cutting-edge radius and a friction force coefficient model embedded with flank wear width, are constructed respectively. By integrating the IUCT, the nonlinear shear/ploughing coefficient and the friction force coefficient, a comprehensive micromilling force model including the tool runout, size effect and tool wear is derived. Experiment results show that the proposed comprehensive model is efficient to predict the micro milling force.
Effect of IL6 -174 G/C Gene Polymorphism on Response to Interleukin-6 Blocking Therapy in Systemic Juvenile Idiopathic Arthritis.
Authors: nada doaa waseem , Gaber Rasha Ahmed , Salem Mohamed Labib , Allam Abdallah El Sayed , Ashkar Doaa Shawky Al 
Published: July 2021

Abstract
Objective: The main objective of this study is to analyze and evaluate the association of IL6-174 G/C gene polymorphisms with response to interleukin-6 blockade in Egyptian patients with systemic juvenile idiopathic arthritis, and to assess the effect of other factors on treatment response as well as long-term efficacy of treatment with Tocilizumab. Methods: Sixty systemic JIA patients (37 males and 23 females with median age at onset 5.2 years) who received IL-6 blockade (Tocilizumab) were recruited. The overall response to treatment with IL-6 blockade was assessed according to: 1) Clinical response, 2) switch (or no switch) to another biological DMARD therapy following IL-6 blockade, 3) achievement of clinically inactive disease within 6 months of IL-6 blockade, 4) improvement in disease activity measured using the modified JADAS-10, and 5) achievement of a glucocorticoid-free state. In addition, basic demographic, laboratory data, characteristics of the disease course and IL6 polymorphisms were assessed.Results: Three IL6 -174 genotypes, including, GG, GC, and CC were found with higher frequencies of GC genotype. These genotypes had non-significant association with the response of sJIA patients to IL-6 blockade in this cohort study. Moreover, a longer time frame from disease onset to diagnosis was associated with poorer long-term treatment response. Conclusion. No significant impact of IL6 -174 G/C gene polymorphisms on treatment response to IL6 blockade therapy in sJIA Egyptian patients, providing evidence of a “window of opportunity” for improved long-term treatment response with shorter time from the disease onset to diagnosis.
The morphology of large-scale debris avalanche deposit in Cianjur plains, West Java, Indonesia
Authors: Setiawan A. , Syafri I. , Yuningsih E.T. , Sudradjat A. , Burhanudinnur M. 
Published: July 2021

Abstract
The large-scale debris avalanche deposit (DAD) process are quite rarely occurring but the impact is catastrophic. Large-scale flank collapse from ancestral Gede volcano resulted in the deposition of debris avalanche deposit that manifested as prominent hummocky hill landform covering 192 km2 area in Cianjur plains, West Java, Indonesia. Although the deposit covers large areas and showing unique volcanic landscape morphology, the study on the debris avalanche deposit is limited. Here we show the result of field reconnaissance, satellite photo and digital elevation model analysis to understand the morphology of large-scale debris avalanche deposit including distribution and morphological characteristics. The interpreted thickness of the deposit ranging from 200-500m, although the hummocky hill with well-rounded texture is only 50–100 m height due to intense erosion in the tropical region with the remaining portion of the megaclast blocks buried. The distribution of hummocky hills indicate that the flow constrained by pre-existing basin configuration. The debris avalanche deposit showing fan-like morphology that characterized block facies located near the fan mouth, while the matrix facies with matrix-supported smaller blocks in the distal part. The mouth of debris avalanche deposit located just south of Cianjur township with the highest concentration of megaclast block as block facies that interpreted as the main depositional axis. The block facies apparently stopped by lineament of SW-NE oriented Cimandiri valley to the southeast and NNE-SSW oriented Cisokan river lineament to the east which interpreted as buried normal fault that considered as the barrier to confine flow of block facies deposition.