bluuebunny/crossref_metadata_2025_split huggingface dataset:
A link between protein acetylation and mitochondrial dynamics under energy metabolism: A comprehensive overview
Authors: Gu Huihui , Yang Kun , Wu Qiong , Shen Zhentong , Li Xinjian , Sun Chao 
Published: June 2021

AbstractCells adjust mitochondrial morphologies to coordinate between the cellular demand for energy and the availability of resources. Mitochondrial morphology is regulated by the balance between two counteracting mitochondrial processes of fusion and fission. Fission and fusion are dynamic and reversible processes that depend on the coordination of a number of proteins and are primarily regulated by posttranslational modifications. In the mitochondria, more than 20% of proteins are acetylated in proteomic surveys, partly involved in the dynamic regulation of mitochondrial fusion and fission. This article focuses on the molecular mechanism of the mitochondrial dynamics of fusion and fission, and summarizes the related mechanisms and targets of mitochondrial protein acetylation to regulate the mitochondrial dynamics of fusion and fission in energy metabolism.
Long noncoding RNAs in triple‐negative breast cancer: A new frontier in the regulation of tumorigenesis
Authors: Thakur Krishan K. , Kumar Aviral , Banik Kishore , Verma Elika , Khatoon Elina , Harsha Choudhary , Sethi Gautam , Gupta Subash C. , Kunnumakkara Ajaikumar B. 
Published: June 2021

AbstractIn recent years, triple‐negative breast cancer (TNBC) has emerged as the most aggressive subtype of breast cancer and is usually associated with increased mortality worldwide. The severity of TNBC is primarily observed in younger women, with cases ranging from approximately 12%–24% of all breast cancer cases. The existing hormonal therapies offer limited clinical solutions in completely circumventing the TNBC, with chemoresistance and tumor recurrences being the common hurdles in the path of TNBC treatment. Accumulating evidence has correlated the dysregulation of long noncoding RNAs (lncRNAs) with increased cell proliferation, invasion, migration, tumor growth, chemoresistance, and decreased apoptosis in TNBC. Various clinical studies have revealed that aberrant expression of lncRNAs in TNBC tissues is associated with poor prognosis, lower overall survival, and disease‐free survival. Due to these specific characteristics, lncRNAs have emerged as novel diagnostic and prognostic biomarkers for TNBC treatment. However, the underlying mechanism through which lncRNAs perform their actions remains unclear, and extensive research is being carried out to reveal it. Therefore, understanding of mechanisms regulating the modulation of lncRNAs will be a substantial breakthrough in effective treatment therapies for TNBC. This review highlights the association of several lncRNAs in TNBC progression and treatment, along with their possible functions and mechanisms.
αB‐crystallin/HSPB2 is critical for hyperactive mTOR‐induced cardiomyopathy
Authors: Wang Lianmei , Wang Fang , Liu Kemei , Long Caifeng , Chen Yi , Li Chunjia , Li Li , Liu Fangming , Zhang Xinyu , Jing Yanling , Wang Yanan , Liang Aihua , Yan Hongbing , Zhang Hongbing 
Published: June 2021

AbstractEven though aberrant mechanistic target of rapamycin (mTOR) signaling is known to cause cardiomyopathy, its underlying mechanism remains poorly understood. Because augmentation of αB‐crystallin and hspB2 was presented in the cortical tubers and lymphangioleiomyomatosis of tuberous sclerosis complex patients, we deciphered the role of αB‐crystallin and its adjacent duplicate gene, hspB2, in hyperactive mTOR‐induced cardiomyopathy. Cardiac Tsc1 deletion (T1‐hKO) caused mouse mTOR activation and cardiomyopathy. Overexpression of αB‐crystallin and hspB2 was presented in the hearts of these mice. Knockout of αB‐crystallin/hspB2 reversed deficient Tsc1‐mediated fetal gene expression, mTOR activation, mitochondrial damage, cardiomyocyte vacuolar degeneration, cardiomyocyte size, and fibrosis of T1‐hKO mice. These cardiac‐Tsc1; αB‐crystallin; hspB2 triple knockout (tKO) mice had improved cardiac function, smaller heart weight to body weight ratio, and reduced lethality compared with T1‐hKO mice. Even though activated mTOR suppressed autophagy in T1‐hKO mice, ablation of αB‐crystallin and hspB2 failed to restore autophagy in tKO mice. mTOR inhibitors suppressed αB‐crystallin expression in T1‐hKO mice and rat cardiomyocyte line H9C2. Starvation of H9C2 cells activated autophagy and suppressed αB‐crystallin expression. Since inhibition of autophagy restored αB‐crystallin expression in starved H9C2 cells, autophagy is a negative regulator of αB‐crystallin expression. mTOR thus stimulates αB‐crystallin expression through suppression of autophagy. In conclusion, αB‐crystallin and hspB2 play a pivotal role in Tsc1 knockout‐related cardiomyopathy and are therapeutic targets of hyperactive mTOR‐associated cardiomyopathy.
β‐Mannanase production by Penicillium citrinum through solid‐state fermentation using açaí residual biomass (Euterpe oleracea)
Authors: Lima Anna C , Silva Diego , Silva Victória , Godoy Mateus , Cammarota Magali , Gutarra Melissa 
Published: June 2021

AbstractBACKGROUNDAçaí core (Euterpe oleracea) is a residual biomass with high mannan content; thus it can become a good inducer for mannanase production by solid‐state fermentation (SSF). β‐Mannanase production from Penicillium citrinum was investigated using açaí seed as inducer and carbon source through SSF, as well as the properties of β‐mannanase in the crude extract.RESULTSIn this study, a two‐stage screening was performed. The P. citrinum strain exhibited high specific enzymatic activity (29 ± 0.2 U mg−1 protein). Nitrogen addition was necessary to improve β‐mannanase activity (an increase of 389% with sodium nitrate). The effect of pH and temperature on β‐mannanase activity in the crude extract was evaluated, with the highest activity found at pH 4.6 and a temperature of 40–60 °C. The enzymes exhibited good thermal stability with 5 h of incubation at 40, 50 and 60 °C, retaining 90%, 55% and 60% of the initial activity, respectively. Furthermore, P. citrinum β‐mannanase exhibited high specificity against ivory nut mannan (234% relative activity), good activity in the presence of a range of metal ions and the ability to hydrolyze locust bean gum, producing mannose and mannooligosaccharides.CONCLUSIONBased on such data, açaí seed can be used as an inducer of β‐mannanase. The P. citrinum crude enzymatic extract, rich in β‐mannanase, has potential industrial applications, especially in the food and feed industries.
Effects of dust deposition from diamond mining on subarctic plant communities and barren‐ground caribou forage
Authors: Watkinson Autumn D. , Virgl John , Miller Valerie S. , Naeth M. Anne , Kim Jaewoo , Serben Kerrie , Shapka Chris , Sinclair Sean 
Published: June 2021

AbstractDust produced from mining has the potential to reduce plant cover, alter plant communities, and increase metal concentrations in vegetation—changes that may affect the amount, type, and quality of forage for barren‐ground caribou (Rangifer tarandus groenlandicus). We quantified dust deposition from Diavik Diamond Mine (Northwest Territories, Canada) and investigated the changes on forage quality, type, and quantity for caribou. From 2002 to 2016, dust deposition was measured, and vegetation cover and richness were assessed in permanent plots established adjacent to the mine and in reference areas 1–6 km from the mine. Lichen was collected from areas up to 100 km from the mine to determine metal concentrations. Dust deposition rapidly decreased within 4 km of the mine. Plant communities adjacent to the mine (within 500 m) had disproportionately increased cover of vascular plants and decreased bryophyte and lichen cover. Lichen sampled within 4 km from the mine had greater metal concentrations than those sampled farther afield. Concentrations of Al in lichen collected within 40 km of the mine exceeded safe exposure limits for consumption, assuming lichen comprised 100% of caribou diet. We conclude that dust deposition from mining is altering adjacent vegetation communities but that such changes to forage are unlikely to cause negative effects to caribou due to reduced lichen intake in summer and autumn, their migratory nature, and avoidance of mine‐influenced areas. However, minimization and reclamation of mine‐related disturbances will be important for maintaining sufficient quality forage and available habitat or space in caribou ranges.
Circular RNA circ_0001588 sponges miR‐211‐5p to facilitate the progression of glioblastoma via up‐regulating YY1 expression
Authors: Wang Qian , Zheng Dahai , Li Yuhan , Zhang Ye , Sui Rui , Chen Yi , Liang Haiyang , Shi Ji , Pan Renlong , Xu Xiaobing , Sun Deyu 
Published: June 2021

AbstractBackgroundAs the most common and detrimental brain tumor with high invasiveness and poor prognosis, glioblastoma (GBM) has severely threatened people's health globally. Therefore, it is of great importance and necessary to identify the molecular mechanisms involved in tumorigenesis and development, thus contributing to potential therapeutic targets and strategies.MethodsThe level of circ_0001588 was detected in 68 pairs of GBM tissues and adjacent normal tissues and human glioma cell lines via a real‐time quantitative reverse transcriptase polymerase chain reaction (qRT‐PCR). Then, the effect of circ_0001588 on the proliferation, migration and invasion of glioma cells was evaluated. In addition, potential downstream targets of circ_0001588 were forecasted by circBANK and Starbase. Their interaction was confirmed by introducing luciferase reporter assays. Moreover, sh‐circ_0001588 transfected U251 cells were used to form tumors in vivo. Finally, the functional mechanism of circ_0001588 was identified by qRT‐PCR, western blotting, xenograft and immunohistochemistry (IHC) assays.ResultsThe expression of circ_0001588 is markedly up‐regulated in GBM tissues and human gliomas cells. Additionally, increased expression of circ_0001588 is positively relevant with poor survival in GBM patients. The down‐regulation of circ_0001588 distinctly inhibits the proliferation, migration and invasion of GBM in vitro, as well as tumor growth in vivo. Moreover, knockdown of circ_0001588 reduces the tumor volume and weight, enhances the relative IHC staining index of E‐cadherin and decreases the relative IHC staining index of Ki‐67, Yin Yang 1 (YY1) and vinmentin in vivo. Mechanistically, circ_0001588 locates in the cytoplasm, which is directly bound with miR‐211‐5p. Furthermore, circ_0001588 can positively regulate YY1 via sponging miR‐211‐5p. Moreover, circ_0001588 accelerates the proliferation, migration and invasion of GBM by modulating miR‐211‐5p/YY1 signaling.ConclusionsThese results illustrate a new circ_0001588/miR‐211‐5p/YY1 regulatory signaling axis in GBM.
Visual monitoring and optical recognition of digoxin by functionalized AuNPs and triangular AgNPs as efficient optical nano‐probes
Authors: Mohammadzadeh Arezoo , Pashazadeh‐Panahi Paria , Hasanzadeh Mohammad 
Published: June 2021

AbstractIn this study, we presented elective, sensitive, and rapid UV‐Vis spectrophotometry and calorimetric assay for the recognition of digoxin. Therefore, cysteamine‐gold nanoparticles (Cys A‐AuNPs) in the presence of cysteine acid amine and Silver nanoparticles in the presence of tetramethyl benzidine and hydrogen peroxide (AgNPs‐TMB [3,3′,5,5′‐tetramethylbenzidine]‐H2O2) were synthesized and utilized as the desired probe. Finally, color variation of probes was observed in the absence and presence of digoxin. Obtained results indicate that the color of Cys A‐AuNPs changed from dark pink to light in the absence and the presence of digoxin, respectively. Also, the color of AgNPs‐TMB‐H2O2 changed from dark blue to light blue, in the absence and the presence of digoxin, respectively. Moreover, UV‐Vis spectroscopies results indicate digoxin with a low limit of quantification of 0.125 ppm in human plasma samples which linear range was 0.125 to 11 ppm.
Interferon‐alpha position in combating with COVID‐19: A systematic review
Authors: Nakhlband Ailar , Fakhari Ali , Azizi Hosein 
Published: June 2021

AbstractThe newborn coronaivus disease 2019 (COVID‐19) pandemic has become the foremost concern of health system worldwide. Interferon typeI (IFN‐I) are among the well‐known antiviruses. Hence IFN‐α have gained much attention as a treatment for COVID‐19 recently. To sum up the efficiency of IFN‐α against COVID‐19, we searched PubMed, SCOPUS, and EMBASE, from the date of genesis to the 1st of October 2020. Discharge from hospital and virus clearance considered as primary and secondary outcomes, respectively. We compared the aforementioned outcomes of patients treated with standard care protocol and the patients treated with IFN‐α in addition to standard care protocol. Out of 356 identified records, 14 studies were subjected for full‐text screening. Finally, a systematic review was performed with inclusion of five studies. Majority of the participants were males (ranged from 43.50% to 90.0%). We found that time of viral clearance and polymerase chain reaction negative (days) in most studies were decreased in the INF‐α + standard care group. The mean days of virus's clearance in INF‐α group and standard group reported 27.3 and 32.43. Likewise, the average days of hospitalization was found also lower in INF‐α group (18.55 vs. 24.36). This study provides a stand to conclude that early administration of INF‐α may be accounted as a promising treatment of COVID‐19.
NADP+‐dependent isocitrate dehydrogenase isozymes from a psychrotrophic bacterium, Psychrobacter sp. strain 13A
Authors: Komura Tatsuhiko , Takada Yasuhiro 
Published: June 2021

AbstractThe genes encoding dimeric and monomeric isocitrate dehydrogenase (IDH) isozymes from a psychrotrophic bacterium, strain 13A (13AIDH‐D and 13AIDH‐M, respectively), were cloned and sequenced. The deduced amino acid sequences of these two IDHs showed high degrees of identity with those of bacteria of genus Psychrobacter. Analysis of the 16S ribosomal RNA gene of the strain 13A revealed that this bacterium is classified to genus Psychrobacter. The optimum temperatures for activities of 13AIDH‐D and 13AIDH‐M were 55°C and 45°C, respectively, indicating that they are mesophilic. On the contrary, 13AIDH‐D maintained 90% of its maximum activity after incubation for 10 min at 50°C, while the 13AIDH‐M activity was completely lost under the same condition. In addition, 13AIDH‐D showed much higher specific activity than 13AIDH‐M. From northern and western blot analyses, the 13AIDH‐D gene was found to be not transcribed under the growth conditions tested in this study. However, the catalytic ability of the mesophilic 13AIDH‐M was concluded to be enough to sustain the growth of strain 13A at low temperatures. Therefore, a novel pattern of the contribution of IDH isozymes in cold‐living bacteria to their growth at low temperatures was confirmed in strain 13A.
Changes in anthropogenic particulate matters and resulting global climate effects since the Industrial Revolution
Authors: Yang Dongdong , Zhang Hua , Wang Zhili , Zhao Shuyun , Li Jiangnan 
Published: June 2021

AbstractIn order to quantify air pollution effects on climate change, we investigated the climate response associated with anthropogenic particulate matters (PMs) by dividing fine PM (PM2.5, particle size ≤2.5 μm) and coarse particulate matter (CPM, particle size >2.5 μm) in great detail in this work, with an aerosol‐climate coupled model. We find that the changes in PM2.5 and CPM are very different and thus result in different, even opposite effects on climate, especially on a regional scale. The column burden of PM2.5 increases globally from 1850 to the present, especially over Asia's southern and eastern parts, whereas the column concentration of CPM increases over high‐latitude regions and decreases over South Asia. The resulted global annual mean effective radiative forcing (ERF) values due to PM2.5 and CPM changes are −1.21 W·m−2 and −0.24 W·m−2, respectively. Increases in PM2.5 result in significant cooling effects on the climate, whereas changes in CPM produce small and even opposite effects. The global annual mean surface air temperature (SAT) decreases by 0.94 K due to PM2.5 increase. Coolings caused by increased PM2.5 are more apparent over Northern Hemisphere (NH) terrain and ocean at mid‐ and high latitudes. Increases in SATs caused by increased CPM are identified over high latitudes in the NH, whereas decreases are identified over mid‐latitude regions. Strong cooling due to increased PM2.5 causes a southward shift of the Intertropical Convergence Zone (ITCZ), whereas the Hadley circulation associated with CPM is enhanced slightly over both hemispheres, along with the weak movement of corresponding ITCZ. The global annual mean precipitation decreases by approximately 0.11 mm day−1 due to the increased PM2.5. Generally, PM2.5 concentration changes contribute more than 80% of the variation caused by all anthropogenic aerosols in ERF, SAT, cloud fraction, and precipitation.
Relationship between the interannual and intraseasonal temperature variability in Northeast China
Authors: Li Wenhui , Tan Guirong , Li Tim 
Published: June 2021

AbstractA significant negative correlation between the seasonal mean temperature anomaly and the intensity of the intraseasonal temperature oscillation in the northern winter over Northeast China is found during the time period 1959–2011. A stronger intraseasonal oscillation (ISO) is found during an extremely cold winter. The cold winter in Northeast China is a part of zonally oriented large‐scale pattern with a negative (positive) temperature anomaly in mid‐ (high) latitudes and is associated with a negative phase of the North Atlantic Oscillation (NAO). The intraseasonal temperature anomaly originates from high‐latitude Central Eurasia and propagates southeastward. The southeastward propagation is attributed to the upper tropospheric dispersion of Rossby wave energy as a result of coupling of the low‐level temperature and the upper‐level geopotential height. The cause of the negative correlation between the intraseasonal and interannual modes is investigated. A cold winter in Northeast China is associated with a weakened meridional temperature gradient and vertical wind shear north of 50°N. This weakens synoptic variability in situ through baroclinic instability. Because the ISO competes for an energy source with the synoptic motion, the ISO is enhanced north of 50°N. The southeastward propagation of the enhanced ISO mode increased intraseasonal temperature variability over Northeast China. As a result, a cold winter in Northeast China is accompanied by an enhanced ISO variability in situ. The enhanced local ISO could further feedback to the winter mean temperature anomaly through nonlinear advective processes. The diagnosis of the temperature budget indicates that the ISO may interact with motion on other scales to contribute to the winter mean temperature anomaly in situ.
Development and validation of a rapid high‐performance thin‐layer chromatographic method for quantification of gallic acid, cinnamic acid, piperine, eugenol, and glycyrrhizin in Divya‐Swasari‐Vati, an ayurvedic medicine for respiratory ailments
Authors: Balkrishna Acharya , Sharma Priyanka , Joshi Monali , Srivastava Jyotish , Varshney Anurag 
Published: June 2021

AbstractDivya‐Swasari‐Vati is a calcium containing polyherbal ayurvedic medicine prescribed for the lung‐related ailments observed in the current pandemic of Severe Acute Respiratory Syndrome Coronavirus 2 infections. The formulation is a unique quintessential blend of nine herbs cited in Ayurvedic texts for chronic cough and lung infection. Analytical standardization of herbal medicines is the pressing need of the hour to ascertain the quality compliance. This persuaded us to develop a simple, rapid, and selective high‐performance thin‐layer chromatographic method for Divya‐Swasari‐Vati quality standardization. The developed method was validated for the quantification of marker components, gallic acid, cinnamic acid, piperine, eugenol and glycyrrhizin, against reference standards in five different batches of Divya‐Swasari‐Vati. The analytes were identified by visualization at 254 nm, and by matching their retention factor with authentic standards. The developed method was validated as per the guidelines recommended by the International Council for Harmonization for parameters like, linearity, limit of detection, limit of quantification, accuracy, and precision. Therefore, the developed novel high‐performance thin‐layer chromatographic process could be employed for rapid standardization of Divya‐Swasari‐Vati and other related herbal formulation, which would aid in quality manufacturing and product development.
Profiling and quantitative analysis of underivatized fatty acids in Chlorella vulgaris microalgae by liquid chromatography‐high resolution mass spectrometry
Authors: Montone Carmela Maria , Aita Sara Elsa , Catani Martina , Cavaliere Chiara , Cerrato Andrea , Piovesana Susy , Laganà Aldo , Capriotti Anna Laura 
Published: June 2021

AbstractChlorella vulgaris is a popular microalga used for biofuel production; nevertheless, it possesses a strong cell wall that hinders the extraction of molecules, especially lipids within the cell wall. For tackling this issue, we developed an efficient and cost‐effective method for optimal lipid extraction. Microlaga cell disruption by acid hydrolysis was investigated comparing different temperatures and reaction times; after hydrolysis, lipids were extracted with n‐hexane. The best recoveries were obtained at 140°C for 90 min. The microalgae were then analyzed by an untargeted approach based on liquid chromatography with high‐resolution mass spectrometry, providing the tentative identification of 28 fatty acids. First, a relative quantification on the untargeted data was performed using peak area as a surrogate of analyte abundance. Then, a targeted quantitative method was validated for the tentatively identified fatty acids, in terms of recovery (78‐100%), intra‐ and interday relative standard deviations (<10 and <9%, respectively) and linearity (R2 > 0.98). The most abundant fatty acids were palmitic, palmitoleic, oleic, linoleic, linolenic, and stearic acids.