bluuebunny/crossref_metadata_2025_split huggingface dataset:
CKAP2L, a Crucial Target of miR-326, Promotes Prostate Cancer Progression
Authors: Li Qi , Yan Mo , Wang Chunhui , Kan Duo , Wang Kaibin , Bao Guochang 
Published: October 2021

Abstract
BackgroundThe overexpression of aberrant cell cycle signaling pathway associated protein has been implicated in multiple malignancies and the identification of all-important one among is the crux of the precise targeted therapy. CKAP2L (Cytoskeleton Associated Protein 2 Like) plays a newish role in cancer progression through activation of the process of cell cycle and mitosis. In this study, we aim to delineate the prominent dysregulated expression of CKAP2L and comprehensively reveal its deregulation in prostate cancer.MethodWe experimentally manipulated CKAP2L gene expression in vitro and in vivo and monitored its effects on cancer-related gene expression, cell migration, proliferation.ResultsIn multiple datasets, CKAP2L was found upregulated and positively associated with Gleason grade and poor clinical outcomes of patients. shRNA mediated silence of CKAP2L suppressed cell proliferation, impaired monolayer formation, inhibited cell invasion. CKAP2L was confirmed to be the direct target of miR-326, which had a carcinostatic effect by binding the 3’untranslated regions (3’UTRs) of CKAP2L mRNA. The deletion of CKAP2L resulted in reduced expression of genes involved in the mitotic cell cycle such as multiple cyclin-dependent kinases and cyclins, but also several genes encoding proteins involved in chromosome segregation and spindle assembly. ConclusionTaken together, miR-326 plays a carcinostatic role in prostate cancer by reducing the expression of CKAP2L .
Hesperidin inhibits biofilm formation, virulence and staphyloxanthin synthesis in methicillin resistant Staphylococcus aureus by targeting SarA and CrtM: An in vitro and in silico approach
Authors: Karuppiah Vijayakumar , Seralathan Muhilvannan , Mani Arunvignesh 
Published: October 2021

Abstract
Methicillin resistant Staphylococcus aureus is considered the multidrug resistant bacterium due to developing biofilm formation associated with antimicrobial resistance mechanisms. Therefore, inhibition of biofilm formation is an alternative therapeutic action to control MRSA infections. The present study revealed the non-antibacterial biofilm inhibitory potential of hesperidin against ATCC strain and clinical isolates of S. aureus. In addition, hesperidin treatment significantly impedes lipase, hemolysin, autolysin, autoaggregation and staphyloxanthin production. Reductions of staphyloxanthin production possibly increase the MRSA susceptibility rate to H2O2 oxidative stress condition. In gene expression study revealed the hesperidin treatment downregulated the biofilm-associated gene (sarA), polysaccharide intracellular adhesion gene (icaA and icaD), autolysin (altA), fibronectin-binding protein (fnbA and fnbB) and staphyloxanthin production (crtM). Molecular docking analysis predicted the ability of hesperidin to interact with SarA and CrtM proteins involved in biofilm formation and staphyloxanthin production in MRSA.
Association Between Dietary Total Antioxidant Capacity and Hypertension in Pre- and Postmenopausal Women; Finding From RaNCD Cohort Study
Authors: Fateh Hawal Lateef , Mirzaei Narmin , Gubari Mohammed Ibrahim Mohialdeen , Darbandi Mitra , Najafi Farid , Pasdar Yahya 
Published: October 2021

Abstract
Background: Diet is one of the influential factors in developing hypertension. Moreover, the role of antioxidants in the prevention of cardiovascular diseases (CVDs) has been identified. Total antioxidant capacity (TAC) is a valid tool to assess intake antioxidants from diet. The aim of this study was to examine the association between TAC and hypertension in pre- and postmenopausal women.Methods: In this cross-sectional study was used baseline data of the Ravansar Non-Communicable Disease (RaNCD) cohort study. The TAC was calculated using food items of the food frequency questionnaire. Univariable and multivariable logistic regression were applied to estimates odds ratio. Results: A total of 5,067 women were included in the study. The mean daily intake of refined grains, legumes, white meat, nuts, omega-3, and omega-6, vitamins of B1, B2, B6 and D were significantly higher among women non-hypertensive compared to hypertensive (P<0.05). Multivariate logistic regression showed the odds of hypertension in the third quartile of dietary TAC was 0.71 (95% CI: 0.52, 0.96) times and the fourth quartile of dietary TAC was 0.73 (95% CI: 0.53, 0.98) times lower compared to the first quartile, in pre/perimenopausal women. There was no association between TAC and hypertension in postmenopausal women.Conclusion: A high dietary TAC was associated to a decreased risk of hypertension in pre/perimenopausal women. We suggest a diet rich in natural antioxidants as it may help prevent developing of hypertension before menopausal.
Transcriptomic Time Series Analysis in Wound Healing: Challenges and Perspectives on Data Interpretation
Authors: Zlobina Ksenia , Malekos Eric , Chen Han , Gomez Marcella 
Published: October 2021

Abstract
Background: Wound transcriptomic analysis can be used to quantify wound healing stages and identify leverage points for wound healing intervention. However, individual gene signatures corresponding to wound healing stages vary from one experiment to another and are highly dependent on both experimental setup and bioinformatics methods. Methods: We develop a systematic approach to informatively compare time series from publicly available wound transcriptomic datasets, including mouse and human wounds, and identify consistent gene expression patterns. Results: We reveal the limitations of gene expression data collection, interpretation, and comparison. For example, the sample rate of wound transcriptomic sample collection must be higher than the rate of changes in the wound healing processes, otherwise, important changes in gene expression may be missed. This may lead to mis finding the most significant genes, as peaks of expression for highly differentially expressed genes are lost. Nevertheless, we derived a short list of genes highly differentially expressed in all datasets under consideration. After clustering and normalization, these genes clearly demonstrate similarly changing dynamics of expression between the wounds and may be used for wound healing stage detection.Conclusions: A list of genes that may be used for transcriptomics-based wound healing stage detection is provided. In addition, we suggest experimental approaches that could help researchers to extract more meaningful results.
Practices of Sickle Cell Disease Genetic Screening and Testing in the Prenatal Population
Authors: Prince Arianna , Cruz-Bendezú Alanna , Gunawansa Nicole , Wade Jenna , Coleman-Cowger Victoria H. , Schulkin Jay , Macri Charles 
Published: October 2021

Abstract
Background

Genetic screening and testing has only recently become an accessible assessment for genetic risk. Unfortunately, these technologies have been underutilized in minority populations despite their usefulness for predicting diseases like sickle cell disease (SCD), often found in African American and Black and Hispanic populations. We surveyed prenatal patients to understand current choices, beliefs and experiences surrounding genetic screening and testing, specifically for sickle cell disease.
Methods

In this cross-sectional survey, we collected information from 322 women during prenatal visits from July 2019 through May 2021. Responses to questions about pregnancy screening and testing practices were analyzed for trends to identify barriers to care and education about testing and screening for sickle cell disease. Patients were asked to rate whether they agree or disagree with statements regarding sickle cell health behaviors. We used χ2 tests to compare categorical variables by self-reported race. Binary logistic regression was used to determine the odds ratios and confidence intervals for each outcome.
Results

Women were a mean (SD) age of 33.3 (6.1). 42.9% of patients self-identified as White while 41.3 of patients self- identified as African American/Black. Screening questions were adjusted for differences in race, insurance, and education levels to show significant differences in responses between African Americans/Blacks and Whites for screening for SCD (p=0.047, OR 95% CI= 0.455 [0.210-0.989]) and plans to meet with genetic counselors (p=0.049, OR 95% CI = 0.299 [0.090-0.993]). Regarding sickle cell health behaviors, only the responses to the statements about if sickle cell is not in their family, then it is likely not in themselves or their children, and partners' opinions about genetic screening remained significant (p=0.011 and p=0.081, respectively).
Conclusions

Our findings suggest gaps in screening, testing, and educational efforts between African American/Black and White patients, as well as differences in opinions regarding pregnancy management with a family history or gestational diagnosis of sickle cell disease. Future research should focus on decreasing these healthcare gaps and improving education that address concerns about SCD for relevant populations.
The structural effects on silver nanoparticles plasma edges in optical reflectance spectra of Ag/Ag2O composites synthesized by oxygen plasma treatment of silver thin films
Authors: Kayed Kamal Hamed 
Published: October 2021

Abstract
In this work, we present the results of a unique study that aims to detect the structural effects on the plasma edges in optical reflectance spectra of Ag/Ag2O composites synthesized by treating silver thin films manufactured by thermal evaporation method with oxygen plasma afterglow. The results showed that, each of the optical reflectance spectra contains two plasma edges, the first (λI) belongs to the surface plasmons of the individual silver nanoparticles, and the second (λL) belongs to the larger silver nanoparticles. In addition, we found that the positions of the plasma edges are linearly related to the positions of the optical absorption peaks, except for high and low oxidation rates cases. On the other hand, taking into account previous work, we obtained indications that, the ratio NI/NL may be a measure of the film's effectiveness in inhibiting bacteria.
Prognostic Nomogram to Predict the Overall Survival of Patients with “driver-gene-negative” Lung Adenocarcinoma (LUAD) and Its’ Biological Basis Deciphering
Authors: Zhu Ying , Yang Hao-Shuai , Shan Shi-Chao , Guo Qi-Kun , Liu Zhen-Guo , Chang Dan-Dan , Luo Honghe , Lei Yi-Yan , Ke Zun-Fu 
Published: October 2021

Abstract
Objectives: Assessing the prognosis of patients with “driver-gene-negative” lung adenocarcinoma (LUAD patients negative for EGFR, KRAS, BRAF, HER2, MET, ALK, RET and ROS1 were identified as “driver-gene-negative”) is of significant clinical importance. We aimed to devise a prognostic nomogram for this LUAD subgroup and to define its biological basis.Materials and methods: Prognostic nomogram were established based on a retrospective study of 294 patients with surgical resected “driver-gene-negative” LUAD at The First Affiliated Hospital of Sun Yat-sen University between September 2003 and June 2015. The concordance index (C-index) and calibration curve were used to determine its predictive accuracy and discriminatory capacity. Patients were classified into low- and high-risk subgroups according to nomogram scores. To define the biological basis, we carried out gene set enrichment analysis in an independent dataset of 49 “driver-gene-negative” LUAD patients.Results: The nomogram built on the independent factors including CTC, age and stage achieved C-index of 0.785 [95% confidence interval (CI) 0.753-0.817] for predicting OS of “driver-gene-negative” LUAD. The calibration curves for OS probabilities showed a good agreement between the nomogram prediction and actual observation. High-risk patients had shorter OS [hazard ratio (HR) = 7.43, 95% CI 5.20-10.42, p<0.0001]. The genetic analysis suggested the biological basis for the role of the predictive model may be due to changes in cell proliferation, metabolism and neurological disease, indicating a relationship between increased proliferative potential and preferential poor prognosis.Conclusions: The prognostic nomogram showed promising prediction efficacy and is expected to predict the prognosis of “driver-gene-negative” LUAD. The easy-to-use nomogram and the enriched pathways can help clinical decision-making, guide follow-up planning, and develop new therapeutic targets.
Single-Cell RNA Sequencing Reveals Tumor Microenvironment Remodeling after Neoadjuvant Immunotherapy in Non-small Cell Lung Cancer
Authors: Hu Junjie , zhang lele , Xia Haoran , Yan Yilv , Zhu Xinsheng , Sun Fenghuan , Sun Liangdong , Li Shuangyi , Li Dianke , Wang Jin , Han Ya , zhang jing , Bian Dongliang , Yu Huansha , Chen Yan , Fan Pengyu , Ma Qiang , Jiang Gening , Wang Chenfei , Zhang Peng 
Published: October 2021

Abstract
Immunotherapy has revolutionized cancer treatment, but most patients are refractory to immunotherapy or acquire resistance. To explore immunotherapy resistance mechanisms, we characterized the transcriptomes of ~92000 single cells from 15 patients with non-small cell lung cancer (NSCLC) during neoadjuvant PD-1 blockade combined with chemotherapy. CD8+ T, natural killer, B, and dendritic cells were activated by therapy. Therapy also promoted differentiation of memory T cells into effector T cells. Macrophages were remodeled into an M0-like phenotype and neutrophils into an aged phenotype. Distinct therapy-induced cancer-cell transcriptomes were associated with clinical response. Major pathologic responders (MPRs) activated antigen presentation via major histocompatibility complex class II (MHC-II). Cancer cells of non-MPRs exhibited overexpression of estrogen metabolism enzymes and elevated serum estradiol. Elevated estradiol activated EGFR signaling and upregulated the expression of VEGFA, which promoted an immunosuppressive microenvironment. FCRL4+FCRL5+ memory B cells and CD16+CX3CR1+ monocytes were identified as biomarkers for positive immunotherapy response.
Are Only A Few Hours Adequate For Hospitalization Following Mini-Incision Open Appendectomy In Pediatric Patients?
Authors: Sookpotarom Paiboon , Ayudhya Vichack Chakrapan Na , Tangsirapat Vorapatu 
Published: October 2021

Abstract
Purpose- A very short hospital length of stay following an open appendectomy in children with acute simple appendicitis has never been mentioned yet in literature. The authors reviewed the outcome of these pediatric patients who were treated with the open technique.Methods -We retrospectively reviewed the medical records of the 115 consecutive patients who underwent open appendectomy from June 2017 to July 2021.Results- There were 84 patients whose appendices were inflammatory or suppurative and appendectomies were done only through McBurney’s point. Of these, the average age was 9.11 ± 2.67 years. The mean length of the incision was 1.95 ± 0.48 centimeters. Nearly one-third (25/85) could be discharged with 3 hours following surgery. Of the remaining cases, 9 patients were discharged within the same day.Conclusion- There were increased percentages of patients who were eligible for our criteria in each year. The confidence of both parents and the therapeutic team on this concept is of paramount importance affecting a shift of paradigm of this treatment of this very common surgical condition.
A case report on uneventful anticoagulation and persistence of type1 respiratory failure post severe COVID-19 infection in a patient of Osler-Weber-Rendu Syndrome.
Authors: Gautam Sachin , Mawari Govind , Daga Mradul Kumar , Kumar Naresh 
Published: October 2021

Abstract
Ever since the WHO's declaration of the SARS-CoV-2 pandemic, the medical literature has been focusing upon the patterns of association of SARS-CoV-2 with different diseases. Patients with Osler-Weber-Rendu Syndrome, also known as, Hereditary hemorrhagic telangiectasia (HHT), presents with recurrent epistaxis, nostril manipulations, incidental detection of multiple AVMs (Arterio-Venous Malformations), and telangiectasias over mucocutaneous tissues and internal organs. In addition, these AVMs are prone to bleed or act as a nidus for thrombus formation apart from other serious complications like chronic hypoxemia, anemia, pulmonary artery hypertension, heart failure, and cerebrovascular disease accidents. Here, we provide a case report of such a patient who was diagnosed with HHT as per 'Curaçao criteria'; having a history of multiple episodes of epistaxis, radiological evidence of AVMs over left calf, pulmonary and hepatic region, multiple telangiectasias in the splenic region and uterine vascular malformations. Upon acquiring severe COVID-19 infection, the patient developed complications like anemia, pulmonary artery hypertension, sepsis, acute kidney injury, and post COVID-19 persistence of type1 respiratory failure. Moreover, the risk-benefit ratio of anticoagulation therapy in such patients with COVID-19 infection is tricky and challenging; however, our patient was prophylactically anti-coagulated with enoxaparin for 12 days with an uneventful outcome.
Keywords: Osler-Weber-Rendu Syndrome, Hereditary hemorrhagic telangiectasia, HHT, Prophylactic Anticoagulation, Covid-19, SARS-CoV-2.
Clinical Analysis on Diagnostic Accuracy of Bosch Vivalytic SARS- CoV-2 Point-of-Care Test and Evaluation of Cycle Threshold at Admission for COVID-19 Risk Assessment
Authors: Heger Lukas Andreas , Elsen Nils , Rieder Marina , Gauchel Nadine , Sommerwerck Urte , Bode Christoph , Duerschmied Daniel , Oette Marc , Ahrens Ingo 
Published: October 2021

Abstract
Background Point-of-care (POC) polymerase chain reaction (PCR) tests have the ability to improve testing efficiency in the Coronavirus disease 2019 (COVID-19) pandemic. However, real-world data on POC tests is scarce.Objective To evaluate the efficiency of a novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) POC test in a clinical setting and examine the prognostic value of on admission cycle threshold (CT) on length of hospital stay (LOS) in COVID-19 patients.Methods Patients hospitalised between January and May 2021 were included in this prospective cohort study. Patients’ nasopharyngeal swabs were tested for SARS-CoV-2 with Allplex™2019-nCoV (Seegene Inc.) real-time (RT) PCR assay and novel POC test (Bosch Vivalytic SARS-CoV-2 [Bosch]) as well as the SARS-CoV-2 Rapid Antigen Test (Roche) accordingly. Clinical sensitivity and specificity as well as inter- and intra-assay variability were analysed. Results 120 patients met the inclusion criteria with 46 (38%) having definite COVID-19 diagnose by RT-PCR. Bosch Vivalytic SARS-CoV-2 POC had a sensitivity of 88% and specificity of 96%. The inter- and intra- assay variability was below 15%. CT Value at baseline was lower in patients with LOS ≥10 days when compared to patients with LOS <10 days (27.82 (±4.648) vs. 36.2 (25.9 - 39.18); p=0.0191). There was a negative correlation of CT at admission and LOS (r[44]s= -.31; p=0.038)Conclusion Our data indicate that POC testing with Bosch Vivalytic SARS-CoV-2 is a valid strategy to identify COVID-19 patients and decrease turnaround time to definite COVID-19 diagnosis. Also our data suggests at admission CT as promising marker for length of hospital stay and possibly severity of disease in COVID-19 patients.
Quality of the Academic Research of a Postgraduate Degree in Surgery from a Public University of Ecuador.
Authors: Pauta Jorge Antonio Delgado , Bravo Franklin Mora 
Published: October 2021

Abstract
Introduction: the lack of solidity of educational research (ER) in a high percentage the loss of integrity in the methodology has been reported. The objective of the present study was to determine the quality of the ERs of the postgraduate surgery in a Public University of Ecuador. With the hypothesis that the acceptable theses are equal to 70%.Methods: the present study reviewed the thesis of surgery at the University of Cuenca, from the period January-2010 to November-2019. The probabilistic sample selection was 48 cases. A 51-question EI quality test was used. The values ​​of each variable were homologated as a percentage. A single sample T-test was performed, bivariate correlations using the Spearman coefficient. Significant values ​​were R> 0.5 and P <0.01. The hypothesis test was performed with Chi square.Results: of 48 randomized theses, the lowest average was in the “Hypothesis” sections (41.6% [35.3-47.8]) and the highest in the “Results” sections (93.5% [91.4-95.7]). The relevance was 97.9% (93.7-102.1). 35 theses (72.9%) were rated as "Acceptable". The Hypothesis and References sections were rated as “Not acceptable” in 70.8% and 79.2%. The "results" section obtained the rating of "Excellent" in 93.8%. The hypothesis was accepted, the group has an acceptable quality equal to 70% ”, Chi2 = 0.2208, P = 0.64.Conclusion: the quality of educational research is equal to the 70% raised in the hypothesis, which highlights a minimum sufficiency rating for the educational research studied in this work.
Dynamic access control and security performance prediction for IoT networking using a novel deep learning technique
Authors: P. Sriramya , Reshmy A.K. , Subhashini R. , Tongkachok Korakod , Pasupulla Ajay Prakash , Algani Yousef Methkal Abd , Balaji S. , Bala B.Kiran 
Published: October 2021

Abstract
Internet of things (IoT) has increased an importance for an area of interest in many devices. Then, the applications such as sensitive home sensors, medical devices, wireless sensors,and other devices are related to IoT network. The transmission of big data is subject to a possible attack that could cause network interruptions and problems with security. The security performance prediction is important for IoT networks to address complicated security issues in real-time which one of the attacks can freely threaten its global performance. Initially,investigate the safety performance of security intelligent prediction techniques is linking with deep learning algorithms into the IoT security risks. This contribution provides a CNN model that improves IoT security risk assessment (SRA) performance. Then, the access control techniques are changed with IoT-like dynamic systems with the number of items spread all over the place. Therefore, dynamic access control models are necessary. Thesedesign not individual use strategies of access but incorporate environmental and real-time data to predict the decision on access. The risk-based access control approach is one of those dynamic models. To decide the access decision, this model assesses the security risk value associated with the access request. This assessment of the model proposed results from the performance and accuracy of IoT networks.