bluuebunny/crossref_metadata_2025_split huggingface dataset:
Salmonella Typhimurium induces NAIP/NLRC4- and NLRP3/ASC-independent, caspase-1/4-dependent inflammasome activation in human intestinal epithelial cells
Authors: Naseer Nawar , Bauer Renate , Zhang Jenna , Brodsky Igor E. , Rauch Isabella , Shin Sunny 
Published: December 2021

AbstractSalmonella enterica serovar Typhimurium is a gram-negative pathogen that causes diseases ranging from gastroenteritis to systemic infection and sepsis. Salmonella uses type III secretion systems (T3SSs) to inject effectors into host cells. While these effectors are necessary for bacterial invasion and intracellular survival, intracellular delivery of T3SS products also enables detection of Salmonella by cytosolic immune sensors. Upon detecting translocated Salmonella ligands, these sensors form multimeric complexes called inflammasomes, which activate caspases that lead to proinflammatory cytokine release and pyroptosis. In particular, the Salmonella T3SS needle, inner rod, and flagellin proteins activate the NAIP/NLRC4 inflammasome in murine intestinal epithelial cells (IECs), which leads to restriction of bacterial replication and extrusion of infected IECs into the intestinal lumen, thereby preventing systemic dissemination of Salmonella. While these processes are studied quite well in mice, the role of the NAIP/NLRC4 inflammasome in human IECs remains unknown. Unexpectedly, we found the NAIP/NLRC4 inflammasome is dispensable for early inflammasome responses to Salmonella in both human intestinal epithelial cell lines and organoids. Additionally, the NLRP3 inflammasome and the adaptor protein ASC are not required for inflammasome activation in Caco-2 cells. Instead, we observed a partial requirement for caspase-1, and a necessity for caspase-4 and GSDMD pore-forming activity in mediating inflammasome responses to Salmonella in Caco-2 cells. These findings suggest that unlike murine IECs, human IECs do not rely on NAIP/NLRC4, and also do not use NLRP3/ASC. Instead, they primarily use caspases-1 and −4 to mediate early inflammasome responses to SPI-1-expressing Salmonella.
Cell cycle-dependent recruitment of FtsN to the divisome in Escherichia coli
Authors: Männik Jaana , Pichoff Sebastien , Lutkenhaus Joe , Männik Jaan 
Published: December 2021

AbstractCell division in Escherichia coli starts with the formation of an FtsZ protofilament network in the middle of the cell, the Z ring. However, only after a considerable lag period do the cells start to form a midcell constriction. The basis of this cell cycle checkpoint is yet unclear. The onset of constriction is dependent upon the arrival of so-called late divisome proteins, among which, FtsN is the last arriving essential one. The timing and dependency of FtsN arrival to the divisome, along with genetic evidence, suggests it triggers cell division. In this study, we used high throughput fluorescence microscopy to quantitatively determine the arrival of FtsN and the early divisome protein ZapA to midcell at a single-cell level during the cell cycle. Our data show that recruitment of FtsN coincides with the initiation of constriction within experimental uncertainties and that the relative fraction of ZapA/FtsZ reaches its highest value at this event. We also find that FtsN is recruited to midcell in two distinct temporal stages with septal peptidoglycan synthesis starting in the first stage and accelerating in the second stage, during which the amount of ZapA/FtsZ in the midcell decreases. In the presence of FtsA*, recruitment of FtsN becomes concurrent with the formation of the Z-ring, but constriction is still delayed indicating FtsN recruitment is not rate limiting, at least under these conditions. Finally, our data support the recently proposed idea that ZapA/FtsZ and FtsN are part of physically separate complexes in midcell throughout the whole septation process.ImportanceIn E. coli, FtsN has been considered a trigger for septal wall synthesis and the onset of constriction. While FtsN is critical for cell division, its recruitment kinetics to midcell has not been characterized. Using quantitative high throughput microscopy, we find that FtsN is recruited to midcell in two temporal stages. The septal cell wall synthesis starts at the first stage and accelerates in the second stage. In the presence of an FtsA mutant defective in self-interaction, recruitment of FtsN to midcell is enhanced, but constriction is still delayed. Our results shed new light on an essential but not rate-limiting role of FtsN in E. coli cell division and also support the view that ZapA/FtsZ and FtsN are part of physically separate complexes in midcell throughout the division process.
Identification of functional residues using machine learning provides insights into the evolution of odorant receptor gene families in solitary and social insects
Authors: Mier Pablo , Fontaine Jean-Fred , Stoldt Marah , Libbrecht Romain , Martelli Carlotta , Foitzik Susanne , Andrade-Navarro Miguel A. 
Published: December 2021

AbstractThe gene family of insect odorant receptors (ORs) has greatly expanded in the course of evolution. ORs allow insects to detect volatile chemicals and therefore play an important role in social interactions, the detection of enemies and preys, and during foraging. The sequences of several thousand ORs are known, but their specific function or ligands have been identified only for very few of them. To advance the functional characterization of ORs, we compiled, curated and aligned the sequences of 3,902 ORs from 21 insect species. We identified the amino acid positions that best predict the response to ligands using machine learning on sets of functionally characterized proteins from the fly Drosophila melanogaster, the mosquito Anopheles gambiae and the ant Harpegnathos saltator. We studied the conservation of these predicted relevant residues across all OR subfamilies and show that the subfamilies that expanded strongly in social insects exhibit high levels of conservation in their binding sites. This indicates that ORs of social insect families are typically finely tuned and exhibit a sensitivity to very similar odorants. Our novel approach provides a powerful tool to use functional information from a limited number of genes to investigate the functional evolution of large gene families.
Role of Mn addition on the general corrosion and pitting corrosion behavior of 13Cr stainless steel
Authors: He Jun , Chen Lin , Su Yanjing 
Published: December 2021

Purpose
The purpose of this study is to elucidate the effect of Mn addition on the corrosion behavior of stainless steel.

Design/methodology/approach
Chronoamperometry, quasi-steady-state polarization and electrochemical impedance spectroscopy were used to investigate the corrosion behavior of Mn added A13Cr-HS sample and original S13Cr samples. In addition, the corrosion product film was characterized by a field emission scanning electron microscope equipped with energy-dispersive spectroscopy and X-ray photoelectron spectroscopy.

Findings
The A13Cr-HS sample with 8 wt.% Mn addition maintained good general corrosion resistance in both acidic and alkaline solutions compared to the original S13Cr sample. Additionally, the A13Cr-HS sample had good pitting resistance in an alkaline solution containing Cl−, but a weaker resistance in an acidic solution.

Originality/value
The influence of Mn addition on the formation mechanism of the passive film was systematically analyzed.
Sizing by optimization of line-start synchronous motor
Authors: Knypiński Łukasz , Gillon Frédéric 
Published: December 2021

Purpose
The purpose of this paper is to develop an algorithm and software for determining the size of a line-start permanent magnet synchronous motor (LSPMSMs) based on its optimization.

Design/methodology/approach
The software consists of an optimization procedure that cooperates with a FEM model to provide the desired behavior of the motor under consideration. The proposed improved version of the genetic algorithm has modifications enabling efficient optimization of LSPMSMs. The objective function consists of three important functional parameters describing the designed machine. The 2-D field-circuit mathematical model of the dynamics operation of the LSPMSMs consists of transient electromagnetic field equations, equations describing electric windings and mechanical motion equations. The model has been developed in the ANSYS Maxwell environment.

Findings
In this proposed approach, the set of design variables contains the variables describing the stator and rotor structure. The improved procedure of the optimization algorithm makes it possible to find an optimal motor structure with correct synchronization properties. The proposed modifications make the optimization procedure faster and more

Originality/value
This proposed approach can be successfully applied to solve the design problems of LSPMSMs.
Sociological traditions as a complementary lens to better understand digital transformation policies
Authors: C. Jackson Nicole , Corpakis Dimitri , Steiber Annika 
Published: December 2021

PurposeThis paper aims to introduce how sociological traditions can provide a complementary, conceptual lens needed to better understand a country’s orientation in its digital transformation policies. While historically sociology has been used to study technological effects, introducing a sociological lens that considers broader macro digital policies can better complement a country’s national innovation system framework by highlighting where forms of acceleration and inertia in digital diffusion may or may not occur.Design/methodology/approachTo formulate this lens, iterative literature reviews were conducted and four major sociological traditions (i.e. Durkheim, Functional-Utilitarian, Marxist and Micro-interactionist) were identified and integrated into one structure. The integrated structure was then applied to the French case of Minitel as a sample application. The French Minitel was selected because it is well-known and due to one of the author’s familiarity with the French culture. The description was based upon secondary data.FindingsThrough the use and application of this lens, the findings reveal that understanding a country’s specific orientation within a sociological tradition can help academics and practitioners determine what accelerates or provides inertia in the diffusion of new digital technologies within a country’s sociological frame. For the French Minitel, two dominant views seem to exist in France, the Durkheim and the Functional-Utilitarian view, which both affected the country’s path dependency in continued investments in Minitel.Research limitations/implicationsWhile policymakers are tasked with the development and implementation of digital transformation policies, a key consideration for both scholars and practitioners on digital policy and governance is to understand the broader macro ramifications of sociological frameworks on the evolving effects of digital transformation. While the authors provide a sample illustration, future research is needed to operationalize this lens and to apply it across various regions and countries in the development of new digital transformation policies.Practical implicationsAs countries face considerable pressure to digitize their economies, policymakers require a better framework to advance the sociological aspects of digitization and its effects upon local institutions and actors in society. The paper provides a complementary lens that can better help them in this regard.Originality/valueTo date, policymakers and governments lack an integrated framework to understand the sociological effects of digital technologies and their diffusions along with their implications on societies such as on the framework of national innovation. The authors provide a sample integrated structure and sample application.
Work from home: understanding boundary management profiles using boundary-fit perspective
Authors: Kashive Neerja , Sharma Brijesh , Khanna Vandana Tandon 
Published: December 2021

Purpose
The recent COVID-19 pandemic has (triggered) lots of interest in work from home (WFH) practices. Many organizations in India are changing their work practices and adopting new models of getting the work done. The purpose of the study to look at the boundary-fit perspective (Ammons (2013) and two factors, namely, individual preferences (boundary control, family identity, work identity and technology stress) and environmental factors (job control, supervisor support and organizational policies). These dimensions are used and considered to create various clusters for employees working from home.

Design/methodology/approach
K-mean clustering was used to do the cluster analysis. Statistical package for social sciences 23 was used to explore different clusters based on a pattern of characteristics unique to that cluster, but each cluster differed from other clusters. Further analysis of variance test was conducted to see how these clusters differ across three chosen outcomes, namely, work-family conflict, boundary management tactics used and positive family-to-work spillover effect. The post hoc test also provided insights on how each cluster differs from others on these outcomes.

Findings
The results indicated four distinct clusters named boundary-fit family guardians, work warriors, boundary-fit fusion lovers and dividers consistent (with previous) research. These clusters also differ across at least two major outcomes like boundary management tactics and positive spillover. The high control cluster profiles like Cluster 3 (boundary-fit fusion lovers) and Cluster 4 (dividers) showed low technostress and higher use of boundary management tactics. Cluster 3 (boundary-fit fusion lovers) and Cluster 1 (boundary-fit family guardians) having high environmental influencers also showed higher positive family-to-work spillover.

Research limitations/implications
Because this study is very specific to the Indian context, a broad generalization requires further exploration in other cultural contexts. The absence of this exploration is one of the limitations of this study. On the culture continuum, countries may vary from being individualistic on one extreme to being collectivistic on the other extreme. Interaction of these two cultural extremities with the individual and the environmental dimension, as espoused in this research, can be examined further in a different cultural setting.

Originality/value
This study has extended the work of Ammons (2013) and added external influencers as a dimension to the individual preferences given by (Kossek 2016), and created the cluster for employees in the Indian context. This study has demonstrated the importance of reduced technostress, and the use of boundary management tactics (temporal and behavioral) leads to positive family-to-work spillover. It has also emphasized the relevance of organization policies and supervisor support for better outcomes in WFH.
Enhancing AW/EP tribological characteristics of biolubricant synthesized from chemically modified cotton methyl-esters by using nanoparticle as additives
Authors: Gul Mustabshirha , Kalam Md. Abul , Mohd Zulkifli Nurin Wahidah , Hj. Hassan Masjuki , Abbas Md. Mujtaba , Yousuf Sumra , Al-Dahiree Omar Sabah , Gaffar Abbas Md. Kamaleldin , Ahmed Waqar , Imran Shahab 
Published: December 2021

Purpose
The purpose of this study is to improve the tribological characteristics of cotton-biolubricant by adding nanoparticles at extreme pressure (EP) conditions in comparison with commercial lubricant SAE-40.

Design/methodology/approach
This research involved the synthesis of cotton-biolubricant by transesterification process and then the addition of nanoparticles in it to improve anti wear (AW)/EP tribological behavior. SAE-40 was studied as a reference commercial lubricant. AW/EP characteristics of all samples were estimated by the four-ball tribo-tester according to the American Society for Testing and Materials D2783 standard.

Findings
The addition of 1-Wt.% TiO2 and Al2O3 with oleic acid surfactant in cotton-biolubricant decreased wear scar diameter effectively and enhanced the lubricity, load-wear-index, weld-load and flash-temperature-parameters. This investigation revealed that cotton-biolubricant with TiO2 nano-particle additive is more effective and will help in developing new efficient biolubricant to replace petroleum-based lubricants.

Research limitations/implications
Cotton biolubricant with TiO2 nano-particles appeared as an optimistic solution for the global bio-lubricant market.

Originality/value
No one has not studied the cotton biolubricant with nanoparticles for internal combustion engine applications at high temperature and EP conditions.
Exploring the impact of the COVID-19 pandemic on approaches to innovation in the consulting industry: a grounded theory pilot study
Authors: Kamning Diane Laurette 
Published: December 2021

PurposeThere is a significant pressure on consulting businesses to produce innovative solutions and to assist their clients in producing innovative solutions for their organizational problems as well. In addition to that challenging need to innovate for survival and competition, as other contemporary firms, consultancies must face the global changes brought by the outbreak of the coronavirus infection since 2019. This qualitative pilot study aimed at exploring the impact of the coronavirus disease 2019 (COVID-19) pandemic on the approaches to innovation in the consulting industry.Design/methodology/approachTriggered from the literature gap on approaches to innovation in consultancies during the unprecedented COVID-19 pandemic, a grounded theory approach was used to generate a theoretical explanation of how the COVID-19 is affecting the strategies and approaches of businesses in harnessing innovation opportunities from the perspectives of four professionals from an information technology (IT) consultancy in the USA.FindingsThe findings of this pilot study showed that organizational leaders' increased responsiveness, a Job-To-Be-Done strategy, organizational support and team adaption are the keys to harvesting dynamic capabilities for better competition, even during global environmental changes.Practical implicationsThis implies that managers remain the main actors in a firm's efforts to harvest dynamic capabilities. Innovation strategists, business leaders and policymakers can confidently work together to implement novel and flexible work settings that integrate both social and economic advancements.Originality/valueTheoretical implications support the sustainable innovation strategy concepts and the Job-To-Be-Done theory. Finally, the substantive theory from this pilot study lays the ground for future research on approaches to innovation in the consulting industry.
Strategies for achieving customer order flexibility – supplier perspective
Authors: Forslund Helena , Mattsson Stig-Arne 
Published: December 2021

PurposeThe purpose of this study is to develop a framework of strategies to achieving customer order flexibility in and related to the order-to-delivery (OTD) process. The purpose is also to investigate how companies prioritize various strategies to achieve customer order flexibility.Design/methodology/approachBased on a literature review, pre-tests and conceptual reasoning, a conceptual framework of strategies related to the order-to-delivery process was developed. The strategies were linked to the order quantity and delivery lead-time flexibility dimensions. This structure resulted in six groups covering enabling as well as remedial strategies. An empirical interview study of ten customer–supplier relationships was conducted.FindingsThe interviews identified additional strategies, thereby expanding the framework. The enabling strategies with the highest median values were “have continuous contact with the customer's purchaser” and “use safety stock of raw materials/semi-finished products”. The remedial strategy with the highest median was “re-plan/re-prioritize the order backlog”. In the delivery sub-process, it was more common to apply remedial strategies for delivery lead-time than for order quantities.Research limitations/implicationsThe developed framework is a contribution to the literature on operational flexibility in and related to the OTD process. It complements existing knowledge by taking a supplier perspective.Practical implicationsSuppliers can use the framework as a tool to understand and systematically achieve better customer order flexibility in and related to the OTD process. Customers can use the framework as a checklist for supplier evaluation and supplier development.Originality/valueFew identified studies include empirical data on customer order flexibility.
Risk of postpartum haemorrhage is associated with ethnicity: A cohort study of 981 801 births in England
Authors: Jardine Jennifer , Gurol‐Urganci Ipek , Harris Tina , Hawdon Jane , Pasupathy Dharmintra , van der Meulen Jan , Walker Kate ,   the NMPA Project Team
Published: December 2021

AbstractObjectiveTo determine the association between ethnic group and risk of postpartum haemorrhage in women giving birth.DesignCohort study.SettingMaternity units in England.SampleA total of 981 801 records of births between 1 April 2015 and 31 March 2017 in a national clinical database.MethodsMultivariable logistic regression analyses with multiple imputation to account for missing data and robust standard errors to account for clustering within hospitals.Main Outcome MeasurePostpartum haemorrhage of ≥1500 ml (PPH).ResultsA total of 28 268 (2.9%) births were complicated by PPH. Risks were higher in women from black (3.9%) and other (3.5%) ethnic backgrounds. Following adjustment for maternal and fetal characteristics, and care at birth, there was evidence of an increased risk of PPH in women from all ethnic minority groups, with the largest increase seen in black women (adjusted OR 1.54, 95% CI 1.45–1.63). The increase in risk was robust to sensitivity analyses, which included changing the outcome to PPH of ≥3000 ml.ConclusionsIn England, women from ethnic minority backgrounds have an increased risk of PPH, when maternal, fetal and birth characteristics are taken into account. Factors contributing to this increased risk need further investigation. Perinatal care for women from ethnic minority backgrounds should focus on preventative measures to optimise maternal outcomes.Tweetable AbstractWomen with an ethnic minority background giving birth in England have an increased risk of postpartum haemorrhage, even when characteristics of the mother, the baby and the care received are taken into account.