bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Reverse Phase Protein Array Based Phospho-antibody Characterization Approach and Its Applicability for Clinical Derived Tissue Specimens
Authors: Wang Nan , Zhang Li , Ying Qi , Song Zhentao , Lu Aiping , Treumann Achim , Liu Zhaojian , Sun Tao , Ding Zhiyong 
Published: August 2021

Abstract
Background: Systematic quantification of phosphoprotein within cell signaling networks in solid tissues remains challenging and precise quantification in large scale samples has great potential for biomarker identification and validation. Methods:We developed a reverse phase protein array (RPPA) based phosphor-antibody characterization workflow by taking advantage of the lysis buffer compatible with alkaline phosphatase (AP) treatment and here termed it as a bottom-up antibody screening that differs from the conventional RPPA antibody validation procedure and applied it onto fresh frozen and formalin-fixed and paraffin-embedded tissue (FFPE) to test its applicability.Results:We tested the feasibility of this method by screening 106 phospho-antibodies with RPPA first followed by western blots on a panel of cell lines and demonstrated that AP treatment could serve as an independent factor that can be adopted for rapid RPPA phospho-antibody selection. We also performed studies on different clinical materials. For fresh frozen (FF) samples, pre-selected highly-specific antibodies showed a desirable data reproducibility and antibody specificity based on AP treatment indicating a potential for fresh tissue-based phospho-protein RPPA profiling. Of further clinical significance, using the same approach, based on two sets of FFPE samples from 63 melanoma and 40 lung cancer patients, we showed great interexperimental reproducibility and significant correlation with pathological markers MelanA for melanoma as well as a panel of lung cancer biomarkers for subtyping (EGFR, Napsin A, p63/p40, TTF1 and CK7) generating meaningful data that match clinical features. Conclusions:Our findings establish a highly efficient approach for phospho-antibody characterization by taking advantage of RPPA whereby the same methodology can be applied for tissue-based proteomics and phosphoproteomics in clinical assay development and application.
Epimedin B Through Enhancing NLRP3 Inflammasome Activation To Induces Idiosyncratic Hepatotoxicity
Authors: Gao Yuan , Shi Wei , Tu Can , Zhao Guanyu , Xu Guang , Xiao Xiaohe , Bai Zhaofang 
Published: August 2021

Abstract
Background: The NLRP3 inflammasome plays a crucial role in the pathogenesis of various human diseases, also idiosyncratic drug-induced liver injury (IDILI). Epimedii Folium (EF) is commonly used for treating bone fractures, joint diseases and some chronic illness, but the EF also could induce IDILI. Several studies have confirmed that EF may induce liver injury by upregulating the activity of the NLRP3 inflammasome. However, the major active constituents of EF have not been well-studied. Results: In the present study, we showed that epimedin B, a major active ingredient of EF, induced the development of IDILI by promoting the activation of the NLRP3 inflammasome. Synergistic induction of mitochondrial reactive oxygen species was a crucial contributor to the promoting effect of epimedin B observed on nigericin- or ATP-induced NLRP3 inflammasome activation. Importantly, epimedin B induced liver injury in the LPS-mediated susceptibility mouse model of IDILI，while specifical NLRP3 inhibitor MCC950 pretreatment completely abrogated the Caspase-1 activation and IL-1β secretion then couldn't induce liver injury. Conclusions: Epimedin B specifically facilitated nigericin- or ATP-induced NLRP3 inflammasome activation and the development of IDILI, which is responsible for EF-induced liver injury. These findings suggest that epimedin B is one of the key constituents of liver injury caused by EF，the content of epimedin B in EF may be a risk factor for IDILI, especially in patients with diseases related to nigericin- or ATP-induced NLRP3 inflammasome activation.
An updated Simpson-Based Method for Chatter Stability Prediction in Milling
Authors: Yan Zhenghu , Zhang Changfu , Jia Jianli , Ma Baoji , Jiang Xinguang , Wang Dong , Wang Wei , Yang Chenxi 
Published: August 2021

Abstract
An updated Simpson-based method (USBM) is presented for milling stability analysis. Firstly, the delay differential equation (DDE) is employed to describe the milling process mathematically. Then, the tooth passing period is divided into two subintervals, i.e., the free and forced vibration intervals. Only the forced vibration interval is divided into many equal small-time intervals. Subsequently, the DDE in the state space is solved based on direct integration. By combining the two-step Simpson method and the semi-discretization method, the state transition matrix of the milling system is constructed. The comparison of convergence rate is conducted to validate the accuracy of the proposed method. The results show that the proposed method converges faster than the benchmark methods. The stability lobe diagrams for the one degree of freedom (one-DOF) and two degrees of freedom (two-DOF) milling systems are also obtained by different methods for further evaluation. Meanwhile, the computation time analysis is also carried out. It is revealed that the proposed USBM has advantages in both accuracy and efficiency. Besides, the proposed method can accurately and efficiently predict the stability of milling with both large and low immersion conditions.
Genistein Enhances the Promoting Effects of Exercise on Anti-Oxidant and Anti- Inflammation Balance and Cardiomyopathy in the Ovariectomized Diabetic Rat
Authors: Nazari-Serenjeh Farzaneh , Vafaie Seyyed Alireza , Khajvand-Abedini Maryam , Salehi Amir Mohammad , Mohammadi Mohammad , Habibi Parisa 
Published: August 2021

Abstract
Purpose: These experiments aimed to analyze the effects of genistein and/or exercise on the expression of miRNA-133, IGF-1, and Bcl-2, Bax plus oxidative stress and inflammation markers in the heart of diabetic ovariectomized rats. Methods: Animals were divided into seven groups (n=8): control, sham, ovariectomy (OVX), diabetic ovariectomized (OD), and diabetic ovariectomized with eight weeks of genistein administration (ODG) and with eight weeks of swimming training (ODS) and with eight weeks of both of them (ODGS). High-fat nutrition and low dose streptozotocin injection were used for induction of diabetes. The effect of those treatments was evaluated by measuring lipid profiles, miRNA-133 gene expression, and Bcl-2, Bax, and IGF-1 protein expression levels. Inflammation and oxidative stress markers levels were also measured.Results: ovariectomy down-regulated cardiac miRNA-133, IGF-1, and Bcl-2 expression levels and increased MDA, OSI, TOS, TNF- α, and NF-κB levels plus a reduction in TAC. Diabetes induced an additive effect on the above-measured factors. Genistein improved oxidative and inflammation conditions and up-regulated miRNA-133, Bcl-2, and IGF-1 expression in OD rats. Genistein also enhanced the positive effect of exercise on miRNA-133, Bcl-2, and IGF-1expression in the heart, along with a reduction in Bax. The combined intervention was associated with improvement in oxidative and inflammation conditions. Histological observation showed some abnormality in cardiac tissue, which was improved by genistein or/and exercise treatment.Conclusion: genistein or/and exercise as a natural replacement therapy could improve diabetic-induced cardiac complications within the heart of ovariectomized rats.
Mining Logical Circuits in Fungi
Authors: Roberts Nic , Adamatzky Andrew 
Published: August 2021

Abstract
Living substrates are capable for nontrivial mappings of electrical signals due to the substrate nonlinear electrical characteristics. This property can be used to realize Boolean functions. Input logical values are represented by amplitude or frequency of electrical stimuli. Output logical values are decoded from electrical responses of living substrates. We demonstrate how logical circuits can be implemented in mycelium bound composites. The mycelium bound composites (fungal materials) are getting growing recognition as building, packaging, decoration and clothing materials. Presently the fungal materials are passive. To make the fungal materials adaptive, i.e. sensing and computing, we should embed logical circuits into them. We demonstrate experimental laboratory prototypes of many-input Boolean functions implemented in fungal materials from oyster fungi P. ostreatus. We characterize complexity of the functions discovered via complexity of the space-time configurations of one-dimensional cellular automata governed by the functions. We show that the mycelium bound composites can implement representative functions from all classes of cellular automata complexity including the computationally universal. The results presented will make an impact in the field of unconventional computing, experimental demonstration of purposeful computing with fungi, and in the field of intelligent materials, as the prototypes of computing mycelium bound composites.
Temperate Climate Malaria in 19th Century Denmark
Authors: Ingholt Mathias Mølbak , Chen Tzu Tung , Hildebrandt Franziska , Pedersen Rasmus Kristoffer , Simonsen Lone 
Published: August 2021

Abstract
Background A now-extinct strain of Plasmodium vivax was endemic in northern Europe until the early 20th century. In light of the emergence of other vector borne diseases in Europe under climate change, temperate climate malaria warrants a re-examination. The purpose of this article is to characterize malaria epidemiology in late-19th century Denmark. Methods Malaria, listed as “koldfeber”, morbidity and mortality data were digitized from Danish medical records during the period 1862-1914. The seasonality and spatial patterns of malaria, and the relationship of the disease with environmental factors such as soil types, clay content and altitude, were studied. In addition, the age, sex, mortality and seasonal patterns of malaria were studied in the high-morbidity period of 1862-1880. Results The highest incidence occurred in coastal eastern Denmark, and malaria was most frequent in the region of Lolland-Falster. Eastern Denmark is characterized with high soil clay contents, and Lolland-Falster has a low altitude. Incidence typically peaked in May and the case fatality ratio was 0.17%. The disease affected both sexes and all age groups except for infants. In 1873, a large spring epidemic occurred following flooding during a storm surge in November 1872.Conclusion The Plasmodium parasite likely survived the temperate-climate winters through dormancy in the human host, followed by hypnozoites reactivation in the spring. This possibly explains why malaria epidemics typically occurred around May. The age-structure of malaria morbidity with high adult prevalence, indicate that malaria was not highly endemic in the late 19th century Denmark. The investigation of the relationship between malaria and environmental factors suggests a close connection with local settings, such as soil types and altitude, and especially in combination with extreme weather events like floods, these factors may have led to larger malaria epidemics.
A Comparative Study of Three Imaging Modalities for the Size Selection of Left Atrial Appendage Closure Device
Authors: Ruan Zhong-bao , Wang Fei , Chen Ge-cai , Zhu Jun-guo , Ren Yin , Li Wei , Jin Kai , Zhu Li 
Published: August 2021

Abstract
Background Accurate preprocedural measurements is important for left atrial appendage closure (LAAC). However, there were limited data comparing tomography angiography (CTA), trans-esophageal echocardiography (TEE) and digital subtraction angiography (DSA) for diagnostic accuracy and the utility for LAAC. This study aimed to compare the results of CTA, TEE and DSA measurements and analyze their accuracy, correlation and consistency in patients who successfully underwent LAAC.Methods 157 non-valvular atrial fibrillation (AF) patients who underwent LAAC were included. The maximum diameter and depth of LAA were recorded by CTA, TEE and DSA. Correlations and agreements were compared. Results All patients were successfully performed LAAC with Watchman device. There was no significant difference in the diameter measurement of LAA ostium between DSA and TEE. Meanwhile, The diameter of LAA ostium obtained by CTA was greater than that of DSA and TEE. There were good correlations between the LAA ostium measured by TEE, CTA, DSA and the Watchman device. DSA measurements and the actual device size were the widest limits of agreement, followed by TEE, CTA measurements were the narrowest limits of agreement. For the LAA depth measurements, mean CTA measurements was higher than that of TEE and DSA. There was no significant difference in the depth measurement among the three imaging modalities. Conclusions CTA, TEE and DSA measurements had good correlations with WATCHMAN device. The ostium diameter and depth of the LAA measured by CTA were greater than those measured by TEE and DSA. The relevance and concordance of CTA measurements were the best.
A 5-Channel DWDM Demultiplexer in C Band on Silicon Photonic Crystal Slab: Design and Numerical Analysis
Authors: Dhandrapati Leninbabu , Hegde Gopalkrishna , Talabattula Srinivas , Tupakula sreenivasulu 
Published: August 2021

Abstract
In this work we propose a novel design of 5-channel DWDM demultiplexer and, numerical analysis of its optimal design parameters and device performance is presented. Photonic crystal hexagonal ring resonators of airholes on a silicon slab are used as channel drop filters. Wavelength controlling is achieved by fine tuning of coupling holes outside each ring resonator. For the proposed design, the resolved wavelengths are in the range of 1535 to 1539nm, where Erbium Doped Fiber Amplifiers are applicable. The average coupling efficiency and cross talk are found to be 72.4% and -18.31dB, respectively. Photonic bandgap computation is performed by using plane wave expansion method and spectral characteristics are obtained by applying 3D finite difference time domain method.
Mathematical Modeling and Estimation for Next Wave of COVID19 in Poland
Authors: M.K. Arti , Wilinski Antoni 
Published: August 2021

Abstract
We investigate the problem of mathematical modeling of new corona virus (Covid19) in Poland and tries to predict the upcoming wave. A Gaussian mixture model is proposed to characterize the COVID-19 disease and to predict a new / future wave of COVID-19. This prediction is very much needed to prepare for medical setup and continue with the upcoming program. Specifically, data related to the new confirmed cases of COVID-19 per day are considered, and then we attempt to predict the data and statistical activity. A close match between actual data and analytical data by using the Gaussian mixture model shows that it is a suitable model to present new cases of COVID-19. In addition, it is thought that there are N waves of COVID-19 and that information for each future wave is also present in current and previous waves as well. Using this concept, predictions of a future wave can be made.
CSF-1-Induced DC-SIGN+ Macrophages Work In The Ovarian Endometriosis
Authors: Li Xiaocui , Hong Wei , Cai Yunlang , Zheng Zhenzhen , An Min 
Published: August 2021

Abstract
BackgroundCSF-1 was found to be accumulated in the lesions and peritoneal fluid of endometriosis patients, and CSF-1 induced THP-1-derived macrophages to polarize toward a suppressive phenotype. Researchers found that macrophages were the predominant cells in the peritoneal fluid (PF) of endometriosis patients, and the primary consensus is that the immune status in the PF of endometriosis patients exhibits a depressed state. Does the cytokine CSF-1 induce monocytes to differentiate into macrophages with a DC-SIGN+ suppressive phenotype in endometriosis?MethodsThe level of CSF-1 in control endometrium (N=11), eutopic endometrium (N=17), and ectopic (N=39) endometrium of endometriosis patients was evaluated by real-time polymerase chain reaction and in the PF of control (N=25) and endometriosis (N=35) patients by enzyme-linked immunosorbent assay. CSF-1 was examined by a MILLIPLEX MAP Mouse Cytokine/Chemokine Magnetic Bead Panel in an in vivo study. DC-SIGN+ suppressive macrophages were detected by immunohistochemical staining of tissues and flow cytometric analysis of the PF of control (N=25) and endometriosis (N=35) patients. The phenotypes and biological activities of the resulting macrophages derived from THP-1 cells induced by CSF-1 were compared by an in vitro coculture system with peripheral blood lymphocytes from normal subjects.Results In this study, we found the proportion of DC-SIGN+ suppressive macrophages was larger in the abdominal immune microenvironment of endometriosis patients. CSF-1 was primarily secreted from the ectopic lesions and peritoneum of mice with endometriosis. And, CSF-1 induced the polarization of macrophages toward a DC-SIGN+ suppressive phenotype; this effect was abolished by the addition of anti-CSF-1R. CSF-1 induced DC-SIGN+ macrophages, leading to a depressed status of peripheral blood lymphocytes, including a high percentage of Treg cells and a low percentage of CD8+ T cells. Similarly, blockade with anti-CSF-1R abrogated this biological effect. This is the first study on the predominant role of DC-SIGN+ suppressive macrophages in the depressed immune status of endometriosis patients.Conclusions This is the first study on the predominant role of DC-SIGN+ suppressive macrophages in the depressed immune status of endometriosis patients. Further study of the mechanism and biological activities of CSF-1-induced DC-SIGN+ suppressive macrophages will enhance our understanding of the physiology of endometriosis and indicate new directions for further study.
Choosing the Adaptive Cardiac Phase for Assessing Cardiac Dimensions Using Cardiac Computed Tomography for Heart Disease
Authors: Wang Li , Zhou Jin-Rong , Chen Dong , Deng Yu-Jiao , Chen Jing 
Published: August 2021

Abstract

Background Choosing a suitable cardiac cycle to measure cardiac chamber dimensions and wall thickness can be a more accurate assessment of cardiovascular disease. Methods Cardiac CT was performed on 137 patients for suspected coronary disease. The parameters of left atrium (LA), left ventricle (LV), right atrium (RA), and right ventricle (RV), as well as the wall thickness of LV were measured in different cardiac phases. The general linear mixed model was used to analyze differences in different phases and the correlation between these parameters and traditional risk factors. ROC analysis was performed to estimate LA enlargement. Results The dimensions of LA, RA, and LV wall thickness achieved the maximum at the phase of 35–45%, and those of LV and RV, at 95–5%. Whereas, the changes of LA-B (antero-posterior diameter), LV-D1 (basal dimension), RA-B (minor dimension) and RV-D2 (mid cavity dimension) were relatively more stable during the cardiac cycle. The maximum LA-B diameter(95%CI 36.92,38.48mm), LV-D1 diameter(95%CI 44.36,45.83mm), RA-B diameter(95%CI 48.75,50.61mm), and RV-D2 diameter(95%CI 30.83,32.84mm) and the maximum interventricular septum thickness( 95%CI 10.79,11.51mm) was acquired. Heart rate (HR) and smoking were potential indicators of LVD2 (mid cavity dimension), while HR and LV myocardial mass were potential indicators of LVD3 (apical-basal dimension). In phase 45%, the cut-off value of LA-B was 37.12mm has high sensitivity of 90.9% for predicting LA enlargement. Conclusion Cardiac chamber dimensions and wall thickness vary with the cardiac phase. Choosing the adaptive cardiac phase for evaluating these parameters obtained by cardiac CT could provide a more accurate clinical measurement. Trial registration retrospectively registered.
The Effect of Hyperbolic Two-temperatures Model on Waves Propagation in a Semi-conductor Medium Containing Spherical Cavities
Authors: Abbas Ibrahim , Hobiny Aatef 
Published: August 2021

Abstract
This article is interested in the study of the carrier density, the redial displacement, the conductive temperature, thermodynamic temperature and the stresses in a semi-conductor material containing a spherical hole. This investigation deals with the photo-thermo-elastic interactions in a semi-conductor medium in the context of the new hyperbolic two-temperatures model with one relaxation time. The Laplace transform technique are used to obtain the problem analytical solution by the eigenvalues methods and the inversions of the Laplace transform were performed numerically. Numerical results for semi-conductor materials are shown graphically and discussed.
Etiological and Epidemiological of Viral Diarrhea Among Children Under the Age of Five in Shenyang From 2018 to 2020
Authors: Dong Xinxin , Qi Ying , Chai Ruiyu , Xu Han , Wang Jin , Wang Yingshuang , Chen Ye , Zhang Linlin , Lu Ying , Chen Huijie , yao yan 
Published: August 2021

Abstract
Diarrhea is one of the leading causes of death among children, especially in the age under five, but little data are available on the etiology of viral diarrhea in Shenyang. To understand the infection status and the relevant epidemiological characteristics of viral diarrhea and to fill gaps of how the distribution of viruses change across Shenyang in children under the age of five with diarrhea, stool specimens of children with diarrhea aged 0-59 months and surveillance data was collected from Sentinel Hospital of Shenyang. Rotavirus, calicivirus, adenovirus, and astrovirus 4 viruses were then analyzed, and the proportion of children who tested positive for each pathogen was calculated and seasonal and spatial patterns for major organisms were determined. Viruses were identified in 47.9% of the 897 samples from children with diarrhea. The main viruses of stool samples were rotavirus (16.9%, predominant type G9P[8]), calicivirus (14.7%, norovirus, predominant type GII ), adenovirus (11.8%), and astrovirus (4.5%). Viral infections were mainly detected in the age of 0-12 months. In the area of Shenyang, Huanggu has the most cases (198, 22.1%), followed by Dadong (137, 15.3%) and Hunnan (135, 15.1%). The positive rate of viruses in patients of different ages, seasons, and regions was not same. Public health entities and the government should develop corresponding measures for different age groups, seasons, and regions.