bluuebunny/crossref_metadata_2025_split huggingface dataset:
Impact of 16S rRNA gene redundancy and primer pair selection on the quantification and classification of oral microbiota in next-generation sequencing
Authors: Regueira-Iglesias Alba , Vázquez-González Lara , Balsa-Castro Carlos , Blanco-Pintos Triana , Vila-Blanco Nicolás , Carreira María José , Tomás Inmaculada 
Published: July 2021

Abstract
Background: The identification, at least at the species level, is highly desirable in 16S rRNA sequencing-based studies of the oral microbiota. However, no study in the oral microbiology field has examined the impact of which primer pair is selected to detect redundant and matching amplicons. Consequently, our aims were to: 1) evaluate the number of 16S rRNA genes in the complete genomes of all the bacterial and archaeal species ever detected in the human oral cavity; and 2) assess how the use of different primer pairs would affect the detection and classification of redundant amplicons and matching amplicons (MA) from different taxa. Results: A total of 709 complete genomes (518 bacteria, 191 archaea) were downloaded from the NCBI database, and their complete 16S rRNA genes were extracted. 94.1% of oral bacteria and 52.59% of oral archaea had more than one 16S rRNA gene in their respective genomes. Next, 33 primer pairs identified in previous research and 6 commonly used in the literature were used against all the genomes to obtain amplicons. Between 46.67%-1.29% of the bacterial species and between 38.89%-4.65% of the archaeal species had MA, affecting relevant genera present in the oral environment such as Actinomyces, Fusobacterium, Lactobacillus, Methanosarcina, Staphylococcus, and Streptococcus. The best primer pairs were (the species coverage with no MA values, SC-NMA; region; primer pair position for Escherichia coli J01859.1): KP_F048-OP_R030 for bacteria (93.55%; 3-7; 342-1079), KP_F018-KP_R063 for archaea (89.63%; 3-9; undefined-1506), and OP_F114_OP_R121 for both bacteria and archaea (92.52%; 3-9; 340-1405). Conclusions : In addition to the 16S rRNA gene redundancy, the considerable presence of matching amplicons must be controlled to ensure the accurate interpretation of microbial diversity data. The SC-NMA is a more useful parameter than the conventional coverage percentage for selecting the best primer pairs. The performance of the primer pairs to detect no MA species increases as the average length of the amplicons increases; none of these being the most widely used primer pairs in the oral literature. The choice of primer pair affects significantly diversity estimates and taxonomic classification, conditioning the comparability of oral microbiome studies using different primer pairs.
Future Health Spending Forecast in Leading Emerging BRICS Markets in 2030 - Health Policy Implications
Authors: Jakovljevic Mihajlo , Lamnissos Demetrios , Westerman Ronny , Chattu Vijay Kumar , Cerda Arcadio 
Published: July 2021

Abstract
Introduction: BRICS leading Emerging Markets are increasingly shaping the landscape of global health sector demand and supply for medical goods and services. BRICS’ share of global health spending and future projections will play a prominent role during upcoming 2020s. The purpose of current research was to examine decades long, underlying historical trends in BRICS’ nations health spending and explore these data as the grounds for reliable forecasting of their health expenditures up to 2030.Methods: BRICS’ health spending data spanning 1995 - 2017 were extracted from IHME’s Financing Global Health 2019 database. Total health expenditure, government, prepaid private and out-of-pocket spending per capita and GDP share of total health spending, were forecasted 2018 - 2030. The ARIMA (Autoregressive Integrated Moving Average) models were used to obtain future projection based on time series analysis.Results: Per capita health spending in 2030 is projected to be: Brazil: $1767 (95% PI: 1615, 1977) ; Russia: $1933 (95% PI: 1549, 2317); India: $468 (95% PI: 400.4, 535) ; China: $1707 (95% PI: 1079, 2334); South Africa $1379 (95% PI: 755, 2004). Health spending %GDP shares in 2030 are projected to be: Brazil: 8.4% (95% PI: 7.5, 9.4) ; Russia: 5.2% (95% PI: 4.5, 5.9) ; India: 3.5% (95% PI: 2.9%,4.1%) ; China: 5.9% (95% PI: 4.9, 7.0) ; South Africa: 10.4% (95% PI: 5.5, 15.3).Conclusions: All BRICS expose long term trend to increase their per capita spending in PPP (purchase power parity) terms. India and Russia are highly likely to maintain stable total health spending GDP% share until 2030. China, as the major driver of global economic growth will be capable of significantly expanding its investment into the health sector across an array of indicators. Brazil is the only large nation whose GDP% share of health expenditure is about to contract substantially during the third decade of the 21st century. The steepest curve of increase in per capita spending until 2030 seems to be attributable to India while Russia should achieve the highest values in absolute terms. Health policy implications of long term trends in health spending indicate the need for Health Technology Assessment dissemination among BRICS ministries of health and national health insurance funds. Matters of cost-effective allocation of limited resources shall remain the core challenge in 2030 as well.
Trends in COVID-19 Infected Cases Based on Panel Data Regression Modelling
Authors: RAJARATHINAM A. , TAMILSELVAN P 
Published: July 2021

Abstract
Background and Objective: The novel coronavirus pandemic, known as COVID-19, could not have been more predictable; thus, the world encountered health crises and substantial economic crises. This paper analysed the trends in COVID-19 cases in October 2020 in four southern districts of Tamil Nadu state, India, using a panel regression model. Materials and Methods: Panel data on the number of COVID-19-infected cases were collected from daily bulletins published through the official website www.stopcorona.tn.gov.in maintained by the Government of Tamil Nadu state, India. Panel data regression models were employed to study the trends. EViews Ver.11. Software was used to estimate the model and its parameters. Results: In all four districts, the COVID-19-infected case data followed a normal distribution. Maximum numbers of COVID-19-infected cases were registered in Kanniyakumari, followed by Tirunelveli, Thoothukudi and Tenkasi districts. The fewest COVID-19 cases were registered in Tenkasi, followed by Tirunelveli, Thoothukudi and Kanniyakumari districts. A random effects model was found to be an appropriate model to study the trend.Conclusion: The panel data regression model is found to be more appropriate than traditional models. The Hausman test and Wald test confirmed the selection of the random effects model. The Jarque-Bera normality test ensured the normality of the residuals. In all four districts under study, the number of COVID-19 infections showed a decreasing trend at a rate of 1.68% during October 2020.
A New Primer Pair for Barcoding Bees (Hymenoptera, Anthophila) Without Amplifying the Homologous Wolbachia Gene
Authors: Bleidorn Christoph , Henze Katharina 
Published: July 2021

Abstract
ObjectivesDNA barcoding became an important method for the identification and monitoring of bees. However, standard primer pairs used for barcoding often result in (co-) amplification of bacterial endosymbionts of the genus Wolbachia, which are widespread among bee species. Here we designed a new primer pair and compared it with the performance of the standard Folmer-primers for small sample set of bees representing the main taxonomic groups of bees.ResultsThe newly designed primer pair (BeeCox1F1/BeeCox1R2) clearly outperformed the standard barcoding primer (LCO1490/HCO2198). By generating barcodes for a small test set of bees we found that the new primer pair produced in all cases clear sequences for unambiguous species identification using BOLD. In contrast, the standard barcoding primers often resulted in the amplification of the homologous Wolbachia gene, which either resulted in a mixed chromatogram signal or identification of the bacterial endosymbiont instead of the host.
Effects of cannabis ingestion on endometriosis-associated pelvic pain and related symptoms.
Authors: Sinclair Justin , Collett Laura , Abbott Jason , Pate David W. , Sarris Jerome , Armour Mike 
Published: July 2021

Abstract
Background

The use of cannabis for symptoms of endometriosis was investigated utilising retrospective archival data from Strainprint Technologies Ltd., a Canadian data technology company with a mobile phone application that tracks a range of data including dose, mode of administration, chemovar and their effect on various self-reported outcomes, including pelvic pain.
Methods

A retrospective, electronic record-based cohort study of Strainprint™ users with self-reported endometriosis was conducted. Self-rated cannabis efficacy, defined as a function of initial and final symptom ratings, was investigated across the included symptom clusters of cramps, pelvic pain, gastrointestinal pain, nausea, depression, and low libido. Cannabis dosage form, dose and cannabinoid ratio information was also recorded.
Results

A total number of 252 participants identifying as suffering endometriosis recorded 16193 sessions using cannabis between April 2017 and February 2020. The most common method of ingestion was inhalation (n = 10914, 67.4%) with pain as the most common reported symptom being treated by cannabis (n = 9281, 57.3%). Gastrointestinal symptoms, though a less common reason for cannabis usage (15.2%), had the greatest self-reported improvement after use. Inhaled forms had higher efficacy for pain, while oral forms were superior for mood and gastrointestinal symptoms. Dosage varied across ingestion methods, with a median dose of 9 inhalations (IQR 5 to 11) for inhaled dosage forms and 1 mg/mL (IQR 0.5 to 2) for other ingested dosage forms. The ratio of THC to CBD had a statistically significant, yet clinically small, differential effect on efficacy, depending on method of ingestion.
Conclusions

Cannabis appears to be effective for pelvic pain, gastrointestinal issues and mood, with effectiveness differing based on method of ingestion. The greater propensity for use of an inhaled dosage delivery may be due to the rapid onset of pain-relieving effects versus the slower onset of oral products. Oral forms appeared to be superior compared to inhaled forms in the less commonly reported mood or gastrointestinal categories. Clinical trials investigating the tolerability and effectiveness of cannabis for endometriosis pain and associated symptoms are urgently required.
Anais 3º Congresso Odontológico Londrinense 9º COUEL
Authors: Borges Hedelson Odenir Iecher , Takahama Junior Ademar , Silva Adriana de Oliveira , Gonini Junior Alcides , Vessoni Barbosa Kasuya Amanda , Pagani Martins Caroline Pagani Martins , Luiz Pereira Stabile Cecília Luiz Pereira Stabile , Cortez Douglas Giordani Negreiros , Nakanishi Fernanda , Stabile Glaykon Alex Vitti , Lino Junior Helion Leão , Fabre Hebert Samuel Carrafa , Favaro Jaqueline , Tanaka Jefferson  Luis Oshiro , Andraus Luciana Prado Maia , Inagaki Luciana Tiemi , Carreiro Luiz Sérgio , Hoepnner Márcio Grama , Pedriali Maria Beatriz Bergonse Pereira , Kasai Maria Luiza Iwakura , Botelho Maria Paula Jacobucci , Caldarelli Pablo Guilherme , Tondelli Pedro Marcelo , Guiraldo Ricardo Danil , Silva Ricardo Henrique Alves da , Pires Willian Ricardo , Chuí Thaís Bedôr Jardim , Silva Ana Julia Furlan da , Takeda Ângela Sayuri Maruyama , Trovo Anna Luiza Januska , Caputo Camila Rossin , Gomes Guilherme Borsato , Pereira Isadora de Lima , Correia Leticia Sassaki , Oliveira Lucas Fernando , Gonçalves Lukas Martyniak Ometi , Bueno Maria Eduarda , Silva Natalia Cristina Candido da , Fernandes Tuane Consalter de Mello , Postigo Victoria , Souza Vitoria Iaros 
Published: July 2021

Editorial
Caros(as) leitores(as)
O Congresso Odontológico Londrinese (COL) é um evento científico realizado pelos graduandos do 5º ano do curso de Odontologia da Universidade Estadual de Londrina (UEL), que tem como objetivo contribuir cientificamente para a formação dos acadêmicos e profissionais da área.
Na sua 9ª edição, a comissão organizadora contou com participação do professor Dr. Hedelson Odenir Iecher Borges como presidente e professores de todas as áreas da Odontologia da UEL, a fim de consolidar uma interação de toda a comunidade acadêmica.
O 3º COL e 9º COUEL foi realizado no período de 19 a 22 de maio de 2021 em um formato 100% online. A grade cientifíca foi composta por palestras ministradas por professores e profissionais de reconhecida importância nacional e internacional, além de apresentações de trabalhos.
Comissão Organizadora
3º Congresso Odontológico Londrinense – 9º COUEL/Edição Online - UEL – Universidade Estadual de Londrina - 2021
Las adaptaciones curriculares de pequeno porte o no significativas en el contexto brasileño
Authors: Heredero Eladio Sebastián 
Published: July 2021

La presente comunicación pretende conceptualizar qué se entiende por adaptaciones curriculares de pequeño porte dentro de la legislación brasileña, organizar todas las respuestas que a
diferentes niveles pueden darse dentro de la escuela inclusiva que en estos momentos vivimos y además aportar ideas y estrategias concretas para organizar esta medida de atención a la diversidad de la manera más eficaz posible. A partir de la reflexión sobre el trabajo en el aula y de la experiencia vivida en contextos escolares brasileños2 sobre la base de una metodología de investigación acción se hacen las aportaciones técnicas para su desarrollo en los centros educativos y se dan estrategias concretas para su organización en las instituciones escolares. Por último, se pretende aportar un documento denominado DACI en el cual se recojan todas las medidas que para un determinado alumno o grupo se quieran hacer a fin de ajustar la respuesta educativa a las necesidades concretas por él planteadas en el contexto del aula y de la escuela.
Macroinvertebrados acuaticos como bioindicadores de las aguas negras tratadas a traves del sistema de eriales de oxidacion
Authors: Ortega-Contreras Judith Yamile 
Published: July 2021

Para la descontaminacion de las aguas servidas de Cucuta en la zona de atalaya ,se esta aplicando un sistema natural para el tratamiento de aguas negras denominados eriales de oxidacion.La efectividad del proceso se determina a traves del analisis de macroinvertebrados acuaticos utilizados como indicadores biologicos de la calidad del agua. Con el fin de identificar los cambios del ecosistema acuatico a traves de su trayectoria por los eriales se establecieron nueve estaciones de muestreo. Se encontraron 38 generos diferentes representantes de los ordenes : Diptera Coleoptera, hemiptera, Odonata, Basommatophora, macroinvertebrados tipicos de aguas contaminadas y moderadamente contaminadas .
Efektivitas strategi pembelajaran means ends analysis (MEA) terhadap kemampuan pemecahan masalah matematika dan self efficacy
Authors: Noviyanti Dwi , Siswanah Emy , Fitriani Ulliya 
Published: July 2021

Tujuan penelitian ini adalah untuk mengetahui keefektifan strategi pembelajaran Means Ends Analysis (MEA) terhadap kemampuan pemecahan masalah matematika dan self efficacy peserta didik kelas VII pada materi segiempat. Jenis penelitian yang digunakan adalah kuantitatif dengan desain penelitian posttest only control design. Populasi penelitian ini adalah peserta didik kelas VII SMP Negeri 1 Kendal tahun pelajaran 2018/2019 yang berjumlah 254 peserta didik. Pemilihan sampel menggunakan cluster random sampling dan didapatkan kelas VII D sebagai kelas eksperimen serta kelas VII H sebagai kelas kontrol. Kelas eksperimen diajar dengan strategi pembelajaran MEA, sedangkan kelas kontrol diajar dengan pembelajaran konvensional.Teknik pengambilan data diperoleh dengan metode tes untuk mengetahui kemampuan pemecahan peserta didik setelah penelitian dan metode angket untuk mengetahui self efficacy peserta didik setelah pembelajaran selesai. Hasil penelitian menunjukkan rata-rata nilai kemampuan pemecahan masalah peserta didik kelas eksperimen sebesar 77.48 dan kelas kontrol 69.059. Sedangkan rata-rata self efficacy peserta didik kelas eksperimen sebesar 70.71 serta kelas kontrol 64.529. Uji hipotesis menunjukkan bahwa penggunaan strategi MEA efektif terhadap kemampuan pemecahan masalah dan self efficacy peserta didik kelas VII SMP Negeri 1 Kendal pada materi segiempat.
TAFSIR KONTEKSTUAL ATAS MODERASI DALAM AL-QUR’AN: SEBUAH KONSEP RELASI KEMANUSIAAN
Authors: Ghozali Mahbub , Rizal Derry Ahmad 
Published: July 2021

This study focused on the meaning of the word moderate in the Qur'an contextually with reference to the QS. [2]: 143 and QS. [5]: 66. The idea of concept on religious moderation was still related to the meaning and urgency of moderate attitude by linking this attitude to the behavior of certain religious people. There were three problems in this study, (1) what was the form of the meaning of wasth and muqtashid? (2) what were the factors behind the meaning? (3) how the contextual meaning was generated. To respond the research problem, this study used a qualitative method using content analysis. This study revealed that the mention of moderate party in the Qur'an was not limited to Muslims, but also refers to other people. This was based on the similarity of meaning between the word wasth which was used to refer to Islam and muqtasid which was used to refer to Jews and Christians. Moderate was thus not identical to religion, but it was oriented to the attitude of humanity owned by everyone regardless of religion. Moderation thus did not always indicate the relationship between religion, but the relationship between human being.
Pengaruh Intellectual Capital Terhadap Nilai Perusahaan Dengan Profitabilitas Sebagai Variabel Intervening
Authors: Putri Atikah Juliani , Agustin Henri , Helmayunita Nayang 
Published: July 2021

This study aims to determine the effect of intellectual capital on firm value and profitability as an intervening variable. This research was conducted on finance companies listed on the Indonesia stock exchange in 2013-2017. The sample is determined by purposive sampling 48 finance companies are obtained. Technique of collecting data is done by documentation technique obtained through IDX official website: www.idx.co.id. Data analysis used is structural equation modeling (SEM) using SmartPLS software ver 3.2.7. The result of the research shows that (1) the intellectual capital positive effect on the firm value (2) the intellectual capital does no effect the firm value through profitability
Implementasi kompetensi pembelajaran sepanjang hayat melalui program literasi di perpustakaan sekolah
Authors: Fadhli Rahmat 
Published: July 2021

Lifelong learning can be developed through literacy programs in school libraries. This study aimed to determine the implementation of lifelong learning competence through literacy programs in school libraries. The research method used a qualitative approach through research methods a descriptive to a librarian, four teachers, and 22 students at Al Haraki Islamic Junior High School, Depok, West Java. Through observation, data collection techniques were carried out using a lifelong learning dimension checklist form, semi-structured and in-depth interviews, and literature studies. Data analysis techniques were data reduction utilizing open coding, data presentation, and concluding. Study results indicated that the librarian organized literacy programs supporting the School Literacy Movement. In practice, the librarian acted as the initiator; the teacher became the facilitator and the librarian's partner in developing literacy programs in school. The literacy program had a positive impact on students, including shaping character, increasing knowledge, developing special talents related to communication and writing, adding experience, building positive relationships between librarians and students, and intellectual recreation. Communication competence in foreign languages was one of the lifelong learning competencies that the school library program did not accommodate. The literacy program's obstacles were managing study time and the roles of parents and family at home. This study concludes that the school library literacy program has supported improving the implementation of lifelong learning competencies for students in several dimensions.
Homage to George E. Palade Cell Protein Secretion in Vascular Biology: Overview and Updates
Authors: Chaldakov George N. , Aloe Luigi , Kádár Anna , Ghenev Peter , Fiore Marco , Pancheva Rouzha Z. , Panayotov Plamen 
Published: July 2021

Abstract
This short overview and updates expresses our brain-and-heart homage to George Emil Palade, “the most influential cell biologist ever”. In his 1971 paper Palade wrote for Albert Claude, the founder of biological electron microscopic method: “Seldom has a field owed so much to a single man”. Herein, we articulate the same words for George Palade, the Teacher of many generations in cell biology research and education. Accordingly, we focus on two paradigm shifts in the cell biology, namely (i) the transition from light to transmission electron microscopy in studying cell protein secretion made by George Palade, and (ii) the transition from contractile to secretory phenotype of vascular smooth muscle cells made by Maria Daria Haust followed and developed by our research group. Altogether, we argue that one of the present challenges in vascular biology is to cultivate secreto-centric thinking and thus further focusing on how we could make the vascular muscle’s secretory pathways work for the benefit of human’s cardiovascular health.
Perspectives on Antiviral Drugs Development in the Treatment of COVID-19
Authors: Rusu Aura , Arbănaşi Eliza-Mihaela , Lungu Ioana-Andreea , Moldovan Octavia-Laura 
Published: July 2021

AbstractThe main objective of this review is to highlight the urgent development of new antiviral drugs against SARS-CoV-2 in the context of the coronavirus pandemic. Antiviral medication against SARS-CoV-2 comprises only remdesivir as an approved drug. Scientists are making considerable efforts to identify other effective antivirals. Investments into thede novodesign of new drugs against the SARS-CoV-2 virus are few. Molnupiravir proved to be effective against the SARS-CoV-2 virus and is very close to approval. Pfizer’s two new compounds (PF-07321332, oral administration and PF-07304814, systemic administration) are in the early stages of development. Two types of methods are preferred to discover new antivirals in a short period. Repositioning of approved drugs for antiviral effect conducted to some clinical results for favipiravir, lopinavir/ritonavir, danoprevir/ritonavir, umifenovir, hydroxychloroquine, camostat and nafamostat. Virtual screening of known molecules’ libraries indicated several compounds that were tested or are being tested in clinical trials. In conclusion, only a few innovative antiviral molecules are in various stages of development. However, the repositioning of many known compounds is being studied, including using virtual screening. The pharmaceutical industry is adapting and reinventing itself so that humanity can face a new pandemic in the future.