bluuebunny/crossref_metadata_2025_split huggingface dataset:
PERAN GURU BK DENGAN LAYANAN INFORMASI DALAM MENGATASI KENAKALAN REMAJA BAGI SISWA KELAS X DI SMK TUNAS BANGSA
Authors: Jannah Syarifah Raudatul , Irhamni Gusti , Fauzi Zainal 
Published: February 2021

Tujuan penelitian ini adalah untuk mengetahui layanan informasi yang berjalan saat ini dan untuk mengetahui peran layanan informasi dalam mengangani kenakalan remaja pada siswa kelas X SMK Tunas Bangsa. Desain penelitian yang digunakan yaitu metode kualitatif.. Adapun jumlah sampel yang dibutuhkan dalam penelitian ini dengan lima orang siswa yaitu dengan meminta bantuan guru BK memilih beberapa siswa untuk mewawancara dan memberikan layanan informasi kepada lima orang siswa. Teknik analisis data yang digunakan yaitu wawancara, dan observasi. Dari hasil penelitian dapat ditari kesimpulan layanan informasi yang deiberikan untuk memberikan pemahaman kepada siswa tentang kenakalan remaja terutama tentang informasi yang dibutuhkan dan bisa membantu siswa dalam mengambil keputusan yang tepat dan ditemukannya bentuk kenalakan remaja yang ada di SMK Tunas Bangsa yaitu terlambat masuk sekolah, membolos, menyontek dan melanggar peratusan sekolah. Sarannya untuk guru bimingan konseling diharapkan lebih memberikan informasi sebnyak-banyaknya kepada siswa terkait dengan kenakalan remaja. ___________________________________________________________________ The purpose of this study was to determine information services currently running and to determine the role of information services in dealing with juvenile delinquency in class X SMK Tunas Bangsa students. The research design used is a qualitative method. The number of samples needed in this study with five students, namely by asking the guidance counselor to select several students to interview and provide information services to five students. The data analysis techniques used were interviews and observation. From the research results can be drawn the conclusion of the information services provided to provide understanding to students about juvenile delinquency, especially about the information needed and can help students in making the right decisions and finding forms of adolescent acquaintance in SMK Tunas Bangsa, namely late entry to school, truancy, cheating and violating school requirements. The suggestion for counseling guidance teachers is that it is hoped that they will provide as much information as possible to students regarding juvenile delinquency.
ANALISIS KESIAPAN DAN EVALUASI PENGELOLAAN LABORATORIUM IPA BERBASIS TEKNOLOGI DI ERA REVOLUSI INDUSTRI 4.0
Authors: Nulngafan Nulngafan , Khoiri Ahmad 
Published: February 2021

Kondisi ideal laboratorium IPA disekolah yang memenuhi PP RI No.19 Tahun 2005 telah menetapkan 8 standar laboratorium dan Permendiknas No 24 Tahun 2007 standar Sarana dan Prasarana pendidikan, namun faktanya tidak demikian kondisi ketersediaan dan kesiapan laboratorium IPA belum berfungsi dengan baik, Desain Based Research (DBR) yang terdiri dari: Identifikasi dan analisis masalah, Perancangan solusi, Siklus berulang dalam pengujian dan penyempurnaan rancangan, serta Refleksi untuk menghasilkan prinsip-prinsip desain dan implementasi. Metode pengumpulan data melalui survei, angket kesiapan dan ketersediaan Laboratorium IPA dan dokumentasi. Teknik analisis data menggunakan analisis validasi produk, keterlaksanaan produk, analisis repons serta keefektifan produk kebijakan yang ditawarkan. Hasil analisis Kesiapan Laboratorium IPA berbasis teknologi kriteria belum mencapai 50% artinya kurang siap, Evaluasi penyelenggaraan Laboratorium IPA tertinggi diperoleh sekolah negeri wilayah kota sebesar 85% kategori sangat baik, sedangkan skor terendah diperoleh sekolah swasta wilayah desa sebesar 66,25% kategori cukup, sedangkan ketersediaan Sarana dan prasarana Laboratorium IPA telah memenuhi standar Permendiknas No 24 Tahun 2007 sebesar 78,3% kategori baik. Perlunya kebijakan penelitian pendidikan untuk dapat mengatasi masalah laboratorium IPA di era revolusi industry 4.0 berupa revitalisasi pengelolaan Laboratorium IPA pada sistem cyber dan laboratorium virtual berbasis Teknologi.
Guidelines for Promoting the Implementation of Pharmaceutical Quality Systems at EAEU Enterprises Through the Management of the Engagement of Production Personnel
Authors: Smirnov V. A. , Goryachkin V. V. , Shestakov V. N. , Abramovich R. A. 
Published: February 2021

Introduction. The publication is devoted to the topical organizational and personnel issues of the implementation of the pharmaceutical quality system (PQS). The relevance of the study is due to the high importance of quality assurance in the development, production and release of drugs into circulation.Aim. The aim: to develop guidelines for organizational and personnel assistance in the implementation of pharmaceutical quality systems at enterprises of the Eurasian Union (EAEU) for the production of medicines (drugs) through the toolkit for engaging production personnel in the development of PQS.Materials and methods. Personnel engagement was assessed based on the engagement index; the benchmarking method was used when studying the impact of the engagement of production personnel on the economic indicators of corporate development (indicators of engagement and related economic indicators of “Akrikhin”, “Ozon” were compared with a panel of similar indicators for 12 pharmaceutical enterprises of the EAEU).Results and discussion. The important role of ensuring an increase in the engagement of production personnel in the development of PQS as a tool for sustainable provision of the effectiveness of the implementation of PQS and standards of good manufacturing practice (GMP) at drug production enterprises is stated. Taking into account the experience of forming quality circles in classical Japanese management, an innovative toolkit for managing the engagement of production personnel is proposed, based on the formation of employee communities, coordination and management of their engagement and development through the corporate portal of a pharmaceutical enterprise, as well as membership in closed clubs of ‘quality leaders’.Conclusion. Management of communities (small groups) of production workers is considered as a tool for improving the organizational and personnel support for the implementation and improvement of PQS / GMP at the EAEU pharmaceutical manufacturers. Through the communities, it is proposed to simultaneously manage the engagement, loyalty and job satisfaction of production personnel; community members, through the exchange of best manufacturing practices, ideas and opinions on the development of PQS and the improvement of nuclear quality assurance practices, will stimulate themselves and other production workers to consistently ensure the quality of manufactured drugs. The expected result is a steady increase in the quality of manufactured drugs, adherence of production personnel to the quality philosophy, creation of conditions for the growth of labour productivity of production personnel of pharmaceutical enterprises.
Documentation of Pharmaceutical Development. Part 1. Study of the Documentation of the Site
Authors: Smekhova I. E. , Shigarova L. V. , Meteleva V. D. , Flisyuk E. V. 
Published: February 2021

Introduction. The trends in the modern pharmaceutical industry demonstrate the need for proper documentation of all stages of the life cycle of a medicinal product to ensure its quality and eliminate risks to patients. Therefore, documenting pharmaceutical development, as the first stage of this cycle, is relevant. It will allow regulating technological processes, product quality indicators, as well as ensuring the integrity of the data received. It is advisable to document the pharmaceutical development taking into account the requirements established at the enterprise where the industrial production of this drug is planned.Aim. Development of documentation for pharmaceutical development within the pharmaceutical quality system of the enterprise. One of the objectives of the study was to analyze the document flow of the pharmaceutical quality system of the enterprise planning the production of suppositories for the proper development of documentation for the pharmaceutical development of a medicinal product in this dosage form.Materials and methods. During the research the following methods were used: content analysis; system analysis; sociological (survey method); SWOT analysis technology; systems approach.Results and discussion. In order to get acquainted with modern trends related to pharmaceutical development and pharmaceutical quality system, at the first stage, an analysis of the ICH (International Council on Harmonization) guidance document Quality Implementation Working Group on Q8, Q9 and Q10 Questions & Answers was carried out. An important preparatory stage for the optimal documentation of a pharmaceutical development was the study of the document flow of the pharmaceutical quality system of an enterprise planning to produce two-component suppositories. For this purpose, in the course of the research, a questionnaire was developed containing questions related to the functioning of the quality system, documentation processes and production. The analysis of the received responses made it possible to obtain information on documenting the pharmaceutical development of medicinal products, as well as to identify the main trends associated with this process within the pharmaceutical quality system.Conclusion. The documentation of the pharmaceutical quality system of the enterprise planning the production of suppositories has been analyzed. The strengths and weaknesses of the documentation are identified, a number of proposals for improving the documentation system are highlighted. Based on the results obtained, proposals will be developed for the optimal documentation of pharmaceutical development and a set of documents for an enterprise planning to produce two-component suppositories will be formed.
Nerve Growth Factor: A Dual Activator of Noradrenergic and Cholinergic Systems of the Rat Ovary
Authors: Benitez Agustin , Riquelme Raul , del Campo Miguel , Araya Camila , Lara Hernan E. 
Published: February 2021

The functioning of the ovary is influenced by the autonomic system (sympathetic and cholinergic intraovarian system) which contributes to the regulation of steroid secretion, follicular development, and ovulation. There is no information on the primary signal that activates both systems. The nerve growth factor (NGF) was the first neurotrophic factor found to regulate ovarian noradrenergic neurons and the cholinergic neurons in the central nervous system. The aim of this study was to determine whether NGF is one of the participating neurotrophic factors in the activation of the sympathetic and cholinergic system of the ovary in vivo and its role in follicular development during normal or pathological states. The administration of estradiol valerate (a polycystic ovary [PCO] phenotype model) increased norepinephrine (NE) (through an NGF-dependent mechanism) and acetylcholine (ACh) levels. Intraovarian exposure of rats for 28 days to NGF (by means of an osmotic minipump) increased the expression of tyrosine hydroxylase and acetylcholinesterase (AChE, the enzyme that degrades ACh) without affecting enzyme activity but reduced ovarian ACh levels. In vitro exposure of the ovary to NGF (100 ng/ml for 3 h) increased both choline acetyl transferase and vesicular ACh transporter expression in the ovary, with no effect in ACh level. In vivo NGF led to an anovulatory condition with the appearance of follicular cysts and decreased number of corpora lutea (corresponding to noradrenergic activation). To determine whether the predominance of a NE-induced polycystic condition after NGF is responsible for the PCO phenotype, rats were exposed to an intraovarian administration of carbachol (100 μM), a muscarinic cholinergic agonist not degraded by AChE. Decreased the number of follicular cysts and increased the number of corpora lutea, reinforcing that cholinergic activity of the ovary participates in controlling its functions. Although NGF increased the biosynthetic capacity for ACh, it was not available to act in the ovary. Hence, NGF also regulates the ovarian cholinergic system, implying that NGF is the main regulator of the dual autonomic control. These findings highlight the need for research in the treatment of PCO syndrome by modification of locally produced ACh as an in vivo regulator of follicular development.
Tick-Tattoo: DNA Vaccination Against B. burgdorferi or Ixodes scapularis Tick Proteins
Authors: Klouwens Michelle J. , Trentelman Jos J. A. , Wagemakers Alex , Ersoz Jasmin I. , Bins Adriaan D. , Hovius Joppe W. 
Published: February 2021

IntroductionBorrelia burgdorferi sensu lato (sl) is the causative agent of Lyme borreliosis. Currently there is no human vaccine against Lyme borreliosis, and most research focuses on recombinant protein vaccines. DNA tattoo vaccination with B. afzelii strain PKo OspC in mice has proven to be fully protective against B. afzelii syringe challenge and induces a favorable humoral immunity compared to recombinant protein vaccination. Alternatively, several recombinant protein vaccines based on tick proteins have shown promising effect in tick-bite infection models. In this study, we evaluated the efficacy of DNA vaccines against Borrelia OspC or tick antigens in a tick-bite infection model.MethodWe vaccinated C3H/HeN mice with OspC using a codon-optimized DNA vaccine or with recombinant protein. We challenged these mice with B. burgdorferi sensu stricto (ss)-infected Ixodes scapularis nymphs. Subsequently, we vaccinated C3H/HeN mice with DNA vaccines coding for tick proteins for which recombinant protein vaccines have previously resulted in interference with tick feeding and/or Borrelia transmission: Salp15, tHRF, TSLPI, and Tix-5. These mice were also challenged with B. burgdorferi ss infected Ixodes scapularis nymphs.ResultsDNA tattoo and recombinant OspC vaccination both induced total IgG responses. Borrelia cultures and DNA loads of skin and bladder remained negative in the mice vaccinated with OspC DNA vaccination, except for one culture. DNA vaccines against tick antigens Salp15 and Tix-5 induced IgG responses, while those against tHRF and TSLPI barely induced any IgG response. In addition, Borrelia cultures, and DNA loads from mice tattooed with DNA vaccines against tick proteins TSLPI, Salp15, tHRF, and Tix-5 were all positive.ConclusionA DNA tattoo vaccine against OspC induced high specific IgG titers and provided near total protection against B. burgdorferi ss infection by tick challenge. In contrast, DNA tattoo vaccines against tick proteins TSLPI, Salp15, tHRF, and Tix-5 induced low to moderate IgG titers and did not provide protection. Therefore, DNA tattoo vaccination does not seem a suitable vaccine strategy to identify, or screen for, tick antigens for anti-tick vaccines. However, DNA tattoo vaccination is a straightforward and effective vaccination platform to assess novel B. burgdorferi sl antigen candidates in a relevant tick challenge model.
Immunological Feature and Transcriptional Signaling of Ly6C Monocyte Subsets From Transcriptome Analysis in Control and Hyperhomocysteinemic Mice
Authors: Yang Pingping , Liu Lu , Sun Lizhe , Fang Pu , Snyder Nathaniel , Saredy Jason , Ji Yong , Shen Wen , Qin Xuebin , Wu Qinghua , Yang Xiaofeng , Wang Hong 
Published: February 2021

BackgroundMurine monocytes (MC) are classified into Ly6Chighand Ly6ClowMC. Ly6Chigh MC is the pro-inflammatory subset and the counterpart of human CD14++CD16+ intermediate MC which contributes to systemic and tissue inflammation in various metabolic disorders, including hyperhomocysteinemia (HHcy). This study aims to explore molecule signaling mediating MC subset differentiation in HHcy and control mice.MethodsRNA-seq was performed in blood Ly6Chighand Ly6ClowMC sorted by flow cytometry from control and HHcy cystathionine β-synthase gene-deficient (Cbs-/-) mice. Transcriptome data were analyzed by comparing Ly6Chighvs. Ly6Clowin control mice, Ly6Chighvs. Ly6ClowinCbs-/-mice,Cbs-/-Ly6Chighvs. control Ly6ChighMC andCbs-/-Ly6Clowvs. control Ly6ClowMC by using intensive bioinformatic strategies. Significantly differentially expressed (SDE) immunological genes and transcription factor (TF) were selected for functional pathways and transcriptional signaling identification.ResultsA total of 7,928 SDE genes and 46 canonical pathways derived from it were identified. Ly6ChighMC exhibited activated neutrophil degranulation, lysosome, cytokine production/receptor interaction and myeloid cell activation pathways, and Ly6ClowMC presented features of lymphocyte immunity pathways in both mice. Twenty-four potential transcriptional regulatory pathways were identified based on SDE TFs matched with their corresponding SDE immunological genes. Ly6ChighMC presented downregulated co-stimulatory receptors (CD2, GITR, and TIM1) which direct immune cell proliferation, and upregulated co-stimulatory ligands (LIGHT and SEMA4A) which trigger antigen priming and differentiation. Ly6ChighMC expressed higher levels of macrophage (MΦ) markers, whereas, Ly6ClowMC highly expressed lymphocyte markers in both mice. HHcy inCbs-/-mice reinforced inflammatory features in Ly6ChighMC by upregulating inflammatory TFs (Ets1andTbx21) and strengthened lymphocytes functional adaptation in Ly6ClowMC by increased expression of CD3, DR3, ICOS, andFos. Finally, we established 3 groups of transcriptional models to describe Ly6Chighto Ly6ClowMC subset differentiation, immune checkpoint regulation, Ly6ChighMC to MΦ subset differentiation and Ly6ClowMC to lymphocyte functional adaptation.ConclusionsLy6ChighMC displayed enriched inflammatory pathways and favored to be differentiated into MΦ. Ly6ClowMC manifested activated T-cell signaling pathways and potentially can adapt the function of lymphocytes. HHcy reinforced inflammatory feature in Ly6ChighMC and strengthened lymphocytes functional adaptation in Ly6ClowMC.
Effectiveness and Efficiency of Non-drug Therapy Among Community-Dwelling Adults With Hypertension in China: A Protocol for Network Meta-Analysis and Cost-Effectiveness Analysis
Authors: Shao Taihang , Li Xia , Zhou Chengchao , Zang Xiao , Malone Daniel C. , Zhang Liang , Zhou Jifang , Tang Wenxi 
Published: February 2021

Introduction: The Chinese government has established a nationwide community-based chronic disease management program since 2009 with hypertension a vital part of it. Though drugs have been proven effective with hypertensive patients, they bring economic burden as well, especially for those who with elevated blood pressure and are potentially eligible for national programs. When the effectiveness of pharmacotherapy-only interventions remains uncertain on these patients, non-pharmacological interventions have demonstrated non-inferior effectiveness and may have economic advantages. To date, there rarely are evidences on the effectiveness and cost-effectiveness of non-pharmacological treatment in comparison with pharmacological interventions for patients with varying severity of blood pressure. This study aims to propose a study for a network meta-analysis and cost-effectiveness analysis to explore what kind of intervention is potentially effective and cost-effective to four specific patient groups, stage I-III hypertensive patients and patients with elevated blood pressure, and to provide recommendations for hypertensive management to Chinese decision makers.Methods: We will systematically search databases (MEDLINE, PubMed, Cochrane Library, etc.,) for randomized controlled trials and observational studies with qualified study design in recent decade that assess the effectiveness of non-pharmacological, pharmacological, or combined intervention aimed at adult populations who are diagnosed with the above four types of hypertension in China. The effectiveness outcomes will include changes in SBP/DBP, rate of comorbidities, mortality, and health related quality of life. We will use network meta-analysis to compare and rank effectiveness of different interventions. Subgroup analyses and meta-regression analyses will be performed to analyze and explain heterogeneity. The economic outcome will include cost-effectiveness based on simulation results from Markov models. Under study perspective of Chinese health system, life-time direct cost will be included.Discussion: This study aims to compare and rank the effectiveness and cost-effectiveness of pharmacological, non-pharmacological and combined interventions for stage I–III hypertensive patients and those who with elevated blood pressure. Compared to existing studies, this comprehensive synthesis of relevant evidences will influence future practice with better efficiency and generalizability for community-based hypertensive management programs in China. The study might also be valuable for other low- and middle-income countries to find their own solutions.PROSPERO registration number: CRD42020151518
EZH2-miRNA Positive Feedback Promotes Tumor Growth in Ovarian Cancer
Authors: Liu Ting , Cai Jian , Cai Jing , Wang Zehua , Cai Liqiong 
Published: February 2021

Enhancer of zester homolog 2 (EZH2), a histone methyl transferase that mediates H3K27me3 through polycomb repressive complex 2 (PRC2), is overexpressed in ovarian cancer and promotes malignant proliferation. However, the underlying mechanism of maintaining high EZH2 expression remains elusive. Here we showed that microRNA(miRNA) inhibited EZH2 by binding to the 3′-UTR of EZH2 mRNA; conversely, EZH2 can inhibit miRNA expression. We confirmed that a feedback loop exists between EZH2 and miRNA that maintained EZH2 overexpression, thus promoting ovarian cancer proliferation in vivo and in vitro. We further explored that EZH2 inhibited miRNA expression through PRC2, as determined by CHIP (chromatin immunoprecipitation), and EZH2 decreased the expression of p21, p53, and RUNX3. These results suggest that EZH2 inhibits the expression of Et-miRNAs (EZH2-targeting miRNAs) through the H3K27me3 pathway, thus forming an EZH2-miRNA positive feedback loop that maintains the high expression of EZH2 and promotes the malignant proliferation of cancer cells by regulating the expression of cell proliferation-related proteins.