bluuebunny/crossref_metadata_2025_split huggingface dataset:
Human umbilical cord blood-mesenchymal stem cell-derived secretome in combination with atorvastatin enhances endothelial progenitor cells proliferation and migration
Authors: Oktaviono Yudi Her , Hutomo Suryo Ardi , Al-Farabi Makhyan Jibril , Chouw Angliana , Sandra Ferry 
Published: May 2021

Background: Human umbilical cord blood-mesenchymal stem cell (hUCB-MSC)-derived secretome is known to be able to promote neovascularization and angiogenesis, so it is also thought to have a capability to modulate endothelial progenitor cell (EPC) functions. Atorvastatin is the cornerstone of coronary artery disease (CAD) treatment which can enhance EPCs proliferation and migration. This study aims to analyze the effect of the hUCB-MSC-derived secretome and its combination with atorvastatin toward EPCs proliferation and migration. Methods: EPCs were isolated from a CAD patient’s peripheral blood. Cultured EPCs were divided into a control group and treatment group of 2.5 µM atorvastatin, hUCB-MSC-derived secretome (2%, 10%, and 20% concentration) and its combination. EPCs proliferation was evaluated using an MTT cell proliferation assay, and EPC migration was evaluated using a Transwell migration assay kit. Results: This research showed that hUCB-MSC-derived secretomes significantly increase EPC proliferation and migration in a dose-dependent manner. The high concentration of hUCB-MSC-derived secretome were shown to be superior to atorvastatin in inducing EPC proliferation and migration (p<0.001). A combination of the hUCB-MSC-derived secretome and atorvastatin shown to improve EPCs proliferation and migration compared to hUCB-MSC-derived secretome treatment or atorvastatin alone (p<0.001). Conclusions: This study concluded that the hUCB-MSC-derived secretome work synergistically with atorvastatin treatment in improving EPCs proliferation and migration.
Compliance of WHO and UNICEF estimates of national immunization coverage (WUENIC) with Guidelines for Accurate and Transparent Health Estimates Reporting (GATHER) criteria
Authors: Danovaro-Holliday M. Carolina , Gacic-Dobo Marta , Diallo Mamadou S. , Murphy Padraic , Brown David W. 
Published: May 2021

Background: The objective of the study was to assess compliance of the WHO and UNICEF estimates of national immunization coverage (WUENIC) against the 18 criteria of the Guidelines for Accurate and Transparent Health Estimates Reporting (GATHER) that define and promote good practice in reporting of global health estimates. Methods: We conducted a desk review of the WUENIC estimation and reporting process vis-à-vis each of the 18 GATHER criteria to complete a self-assessment of compliance with GATHER. Results: Overall, WUENIC estimates are fully compliant with 17 of the GATHER criteria and partially compliant with one criterion—criterion 11, which is related to candidate model evaluation and final model selection. Conclusion: The GATHER criteria provide a useful framework for documenting WUENIC’s compliance with contemporary reporting requirements. Given the role of vaccination coverage estimates in global monitoring and guiding disease control efforts, WHO and UNICEF strive to produce and publish robust estimates of vaccination coverage through a transparent process that emphasizes country involvement.
A study protocol of qualitative data sharing practices in clinical trials in the UK and Ireland: towards the production of good practice guidance
Authors: Houghton Catherine , McCarthy Megan , Gillies Katie , Rousseau Nikki , Wade Julia , Gamble Carrol , Toomey Elaine , Matvienko-Sikar Karen , Sydes Matthew , Dowling Maura , Bryant Val , Biesty Linda 
Published: May 2021

Background: Data sharing enables researchers to conduct novel research with previously collected data sets, thus maximising scientific findings and cost effectiveness, and reducing research waste. The value of sharing anonymised data from clinical trials is well recognised with a moderated access approach recommended. While substantial challenges to data sharing remain, there are additional challenges for qualitative data. Qualitative data including videos, interviews, and observations are often more readily identifiable than quantitative data. Existing guidance from UK Economic and Social Research Council applies to sharing qualitative data but does not address the additional challenges related to sharing qualitative data collected within trials, including the need to incorporate the necessary information and consent into already complex recruitment processes, with the additional sensitive nature of health-related data. Methods: Work package 1 will involve separate focus group interviews with members of each stakeholder group: trial managers, clinical trialists, qualitative researchers, members of research funding bodies and trial participants who have been involved in qualitative research. Data will be analysed using thematic analysis and managed within QSR NVivo to enhance transparency. Work package 2 will involve a documentary analysis of current consent procedures for qualitative data collected as part of the conduct of clinical trials. We will include documents such as participant information leaflets and consent forms for the qualitative components in trials. We will extract data such as whether specific clauses for data sharing are included in the consent form. Content analysis will be used to analyse whether and how consent is being obtained for qualitative data sharing. Conclusions: This study will provide insight into the existing practice of sharing of qualitative data in clinical trials and the current issues and opportunities, to help shape future research and development of guidance to encourage maximum learning to be gained from this valuable data.
Tuning the emission color of a quantum emitter by using photonic local density of states
Authors: Chen Gong , Li Xiaoguang 
Published: May 2021

Quantum emitters characterized by their emission colors constitute important elements in the design of modern nano-optics. Although we can change the emission colors of a quantum emitter by tailoring its chemical component, once selected, the color usually cannot be changed. It will be tempting to find out whether the emission color of an emitter could be tuned without touching its chemical component. In this Letter, we theoretically propose a strategy to externally tune the emission color of a model emitter by changing its electromagnetic environment. We found that the photonic local density of states (PLDOS) strongly affect the competition between various internal radiative and nonradiative channels, thus enabling a selective electronic state to dominate the emission spectrum. Indeed, quantitative calculations show that the emission color of a model emitter could be tuned from red to green and blue as the PLDOS increases. Moreover, due to direct correspondence between the emission color and PLDOS, the emitter can be potentially used as a sensor to characterize the local electromagnetic environment by its emission color at the nanoscale. This simple strategy may prove to be useful in the future design of various nano-optical devices.
Temporal and spectral unmixing of photoacoustic signals by deep learning
Authors: Zhou Yifeng , Zhong Fenghe , Hu Song 
Published: May 2021

Improving the imaging speed of multi-parametric photoacoustic microscopy (PAM) is essential to leveraging its impact in biomedicine. However, to avoid temporal overlap, the A-line rate is limited by the acoustic speed in biological tissues to a few megahertz. Moreover, to achieve high-speed PAM of the oxygen saturation of hemoglobin, the stimulated Raman scattering effect in optical fibers has been widely used to generate 558 nm from a commercial 532 nm laser for dual-wavelength excitation. However, the fiber length for effective wavelength conversion is typically short, corresponding to a small time delay that leads to a significant overlap of the A-lines acquired at the two wavelengths. Increasing the fiber length extends the time interval but limits the pulse energy at 558 nm. In this Letter, we report a conditional generative adversarial network-based approach that enables temporal unmixing of photoacoustic A-line signals with an interval as short as

∼

38

n
s

, breaking the physical limit on the A-line rate. Moreover, this deep learning approach allows the use of multi-spectral laser pulses for PAM excitation, addressing the insufficient energy of monochromatic laser pulses. This technique lays the foundation for ultrahigh-speed multi-parametric PAM.
Management of childhood and adolescent latent tuberculous infection (LTBI) in Germany, Austria and Switzerland
Authors: von Both Ulrich , Gerlach Philipp , Ritz Nicole , Bogyi Matthias , Brinkmann Folke , Thee Stephanie 
Published: May 2021

Background
Majority of active tuberculosis (TB) cases in children in low-incidence countries are due to rapid progression of infection (latent TB infection (LTBI)) to disease. We aimed to assess common practice for managing paediatric LTBI in Austria, Germany and Switzerland prior to the publication of the first joint national guideline for paediatric TB in 2017.

Methods
Online-based survey amongst pediatricians, practitioners and staff working in the public health sector between July and November 2017. Data analysis was conducted using IBM SPSS.

Results
A total of 191 individuals participated in the survey with 173 questionnaires included for final analysis. Twelve percent of respondents were from Austria, 60% from Germany and 28% from Switzerland. Proportion of children with LTBI and migrant background was estimated by the respondents to be >50% by 58%. Tuberculin skin test (TST) and interferon-γ-release-assay (IGRA), particularly Quantiferon-gold-test, were reported to be used in 86% and 88%, respectively. In children > 5 years with a positive TST or IGRA a chest x-ray was commonly reported to be performed (28%). Fifty-three percent reported to take a different diagnostic approach in children ≤ 5 years, mainly combining TST, IGRA and chest x-ray for initial testing (31%). Sixty-eight percent reported to prescribe isoniazid-monotherapy: for 9 (62%), or 6 months (6%), 31% reported to prescribe combination therapy of isoniazid and rifampicin. Dosing of isoniazid and rifampicin below current recommendations was reported by up to 22% of respondents. Blood-sampling before/during LTBI treatment was reported in >90% of respondents, performing a chest-X-ray at the end of treatment by 51%.

Conclusion
This survey showed reported heterogeneity in the management of paediatric LTBI. Thus, regular and easily accessible educational activities and national up-to-date guidelines are key to ensure awareness and quality of care for children and adolescents with LTBI in low-incidence countries.
Prenatal y póstumo: Escrituras impotentes en Invenciones del recuerdo de Silvina Ocampo
Authors: Magallanes Romina 
Published: May 2021

Invenciones del recuerdo, de Silvina Ocampo, forma parte del corpus póstumo de la autora. En 2006 Sudamericana editó por primera vez esta obra al cuidado de Ernesto Montequin, que ha sido considerada como un texto autobiográfico.
Este trabajo propone una lectura de Invenciones del recuerdo que no aborda la obra desde esa perspectiva sino como un texto que expone una impotencia escrituraria a partir de la expresión «prenatal» que abre un arco hasta la construcción póstuma que lo culmina, también en la forma de la impotencia. Siguiendo la lectura que Giorgio Agamben realiza de dicha noción en Aristóteles entendiendo por ella la capacidad de privación, de no pasar al acto, de suspenderse en la posibilidad y resistir a la realización totalizadora de una forma determinada, esta lectura encuentra que Invenciones del recuerdo se mueve en zonas de potencias donde se disuelve el Sujeto que vendría a narrar una vida pasada —autobiografía— para dar cuenta de una composición imprevista con lo «lo sido» (Heidegger ) y donde la «invención», el «instinto», la «infancia», lo póstumo y la edición la afectan.
Para desarrollar estas hipótesis dividiremos el trabajo en siete apartados que analizan y descomponen la noción de «prenatal» en matices que Ocampo presenta hasta legar a su relación con la naturaleza póstuma de esta escritura.
LA GRAVITACION COMO UN MODELO DE CUERDA Y SU CUANTIZACION EN EL ESPACIO DE MINKOWSKI
Authors: Villegas Silva Fulgencio 
Published: May 2021

Se	considera las ecuaciones de campo gravitacional de Einstein, identificándolas con las funciones que describen la inmersión del espacio-tiempo en un espacio plano encontrando así un modelo de teoría de campo. Se hace uso del Lagrangiano de Hilbert de tal manera que los campos dinámicos midan la curvatura del espacio-tiempo donde el tensor energía-momento es proporcional al tensor de Einstein.
Some Results on a Generalized Version of Congruent Numbers
Authors: Casinillo Leomarich F. , Casinillo Emily L. 
Published: May 2021

AbstractThis paper aims to construct a new formula that generates a generalized version of congruent numbers based on a generalized version of Pythagorean triples. Here, an elliptic curve equation is constructed from the derived generalized version of Pythagorean triples and congruent numbers and gives some new results.Keywords: Pythagorean triple, congruent number, elliptic curve equation. AbstrakArtikel ini bertujuan untuk mengkonstruksi formula baru yang membangun versi yang lebih umum dari bilangan-bilangan kongruen berdasarkan versi triple Pythagoras yang diperumum. Di sini, akan dikonstruksi suatu persamaan kurva eliptik dari triple Pythagoras dan bilangan-bilangan kongruen dalam versi yang diperumum untuk menghasilkan hasil-hasil yang baru.Kata kunci: triple Phytagoras, bilangan kongruen, persamaan kurva eliptik.2010 Mathematics subject classification: 11A07, 11A41, 11D45, 11G07.
Space-Time and Motion to Advection-Diffusion Equation
Authors: Ghani Mohammad 
Published: May 2021

AbstractWe are concerned with the study the differential equation problem of space-time and motion for the case of advection-diffusion equation. We derive the advection-diffusion equation from the conservation of mass, where this can be represented by the substance flow in and flow out through the medium. In this case, the concentration of substance and rate of flow of substance in a medium are smooth functions which is useful to generate advection-diffusion equation. A special case of the advection-diffusion equation and numerical results are also given in this paper. We use explicit and implicit finite differences method for numerical results implemented in MATLAB.Keywords: advection-diffusion; space-time; motion; finite difference method. AbstrakKami tertarik untuk mempelajari masalah persamaan diferensial ruang-waktu, dan gerak untuk kasus persamaan adveksi-difusi. Kita menurunkan persamaan adveksi-difusi dari kekekalan massa, di mana hal ini dapat diwakili oleh aliran zat yang masuk dan keluar melalui media. Dalam hal ini konsentrasi zat dan laju aliran zat dalam suatu medium merupakan fungsi halus yang berguna untuk menghasilkan persamaan adveksi-difusi. Sebuah kasus khusus persamaan adveksi-difusi dan hasil numerik juga diberikan dalam makalah ini. Kami menggunakan metode beda hingga explisit dan implisit untuk hasil numerik yang diimplementasikan dalam MATLAB.Kata kunci: adveksi-difusi; ruang-waktu; gerak; metode beda hingga.
Еstrogen receptor α (ESR1) and SRC family kinase (LYN) gene's mutations associated with ovarian cancer endocrine therapy resistance
Authors: Shestakova E. A. 
Published: May 2021

Recently multiple data accumulated concerning mutations in the ESR1 gene coding estrogen receptor α (mutESR1) and in the LYN gene coding non receptor tyrosine kinase SRC family member (mutLYN) that are associated with endocrine therapy resistance and that could be considered as markers of endocrine therapy efficiency. In case of gynecologic cancers including ovarian cancer the most frequent mutESR1 are ESR1L536H/P/R/V , ESR1Y537S/N/C/H, ESR1D538G that emerge in the course of hormonotherapy especially using aromatase inhibitors. mutLYN including LYNE159K, LYND189Y, LYNK209N, LYNA370T, LYNG418R, LYNA503D are also identified. mutESR1 and mutLYN increase transcriptional activity of estrogen receptor α (ERα) coded with ESR1 gene and catalytic activity of LYN kinase inducing endocrine therapy resistance. Interdependence of ESR1 and LYN genes is revealed at the level of proteins that they code as the kinases of the SRC family including LYN activate ERα-dependent transcription due to the phosphorylation of ERα at Y537 amino-acid residue that is the most frequently mutated in tumors with endocrine therapy resistance. The aim of the review is revealing the clinical correlations of mutESR1 and mutLYN with the ovarian cancer endocrine therapy resistance that opens perspectives of mutESR1 and mutLYN use as new predictive markers of ovarian cancer and development of more efficient anti-tumor medicaments. In the review the information obtained from PubMed database for the last 20 years using the following key words: ESR1, LYN, mutation(s), estrogen receptor α (ERα), LYN kinase, SRC family kinases, ovarian cancer, gynecologic(al) cancer is discussed.
Neurofibromatosis type 1: results of our own study (Republic of Bashkortostan)
Authors: Mustafin R. N. , Bermisheva M. A. , Valiev R. R. , Khusnutdinova E. E. 
Published: May 2021

Introduction. Neurofibromatosis type 1 (NF1) is the most common hereditary tumor syndrome (frequency of its occurrence in the world is 1 : 3000 of the population). The main clinical manifestations of the disease are multiple café-au-lait macules on the skin and neurofibromas, skeletal abnormalities and cognitive deficits. The disease is based on mutations in the oncosuppressor gene NF1. This disease is characterized by significant clinical polymorphism of manifestations, even among members of the same family. No geno-phenotypic correlations were found for NF1. Therefore, it is assumed that modifier genes are the cause of the varying expressiveness of the disease. Materials and methods. Clinical-epidemiological and molecular-genetic research of patients with NF1 in the Republic of Bashkortostan (RB) was carried out. Sequencing was used to search for intragenic mutations in 57 exons of the NF1 gene. Microsatellite analysis was used to detect the deletion of the entire gene.Results. The frequency of occurrence of NF1 in RB was 1 : 10153 of the population. Analysis of the clinical manifestations of NF1 in RB patients showed a lower incidence of brain cysts in patients born in mixed marriages, which indicates the protective role of mestization. In patients with NF1 who inherited the disease from the mother, a more frequent development of skeletal anomalies and facial dysmorphism was determined. We identified 1 deletion of the entire NF1 gene in 1 patient and 14 intragenic mutations (c.205-1G>C, с.1278G>A, c.1369_1370insGGGTC, с.1570G>A, с.1973_1974delTC, c.2806A>T, с.2991-1G>C, c.3158C>G, с.3526_3528delAGA, с.3826delC, с.4514+5G>A, с.4537С>Т, c.5758_5761delTTGA, с.6792С>A) in 20 patients with NF1. We determined the random distribution of the types of mutations and did not reveal the specific features of the NF1 clinic depending on the type of mutations.Conclusions. The protective role of crossbreeding in relation to brain cysts, as well as the predominance of skeletal anomalies in patients with NF1 inheritance from the mother, indicate the role of modifier genes in the pathogenesis of the disease. The identified mutations in the NF1 gene will allow us to perform prenatal prevention of NF1 in RB patients.
Studying of the effect of the genetic variant c.470T>C in the CHEK2 gene on increasing the risk of breast cancer in the population of the Russian Federation
Authors: Novikova E. I. , Bozhenko V. K. , Kudinova E. A. , Solodkiy V. A. 
Published: May 2021

Introduction. Currently, there are conflicting data regarding the effect of the c.470T> C germline mutation in the CHEK2 gene on increasing the risk of breast cancer (BC), so it is necessary to conduct research on large samples of patients, including in the Russian population, in order to analyze the contribution of this mutation to the risk of cancer developing.The aim of the study was to determine the frequency of occurrence of the genetic variant c.470Т>С in the CHEK2 gene in the Russian population in patients with BC and patients with benign breast diseases (BBD) to assess the possible effect of this deoxyribonucleic acid damage on the likelihood of cancer occurrence.Materials and methods. The study included 2,787 patients with BC and 1,004 patients with BBD who underwent examination and treatment at the Russian Scientific Center of Roentgenoradiology of the Ministry of the Russian Federation from 2010 to 2018. Molecular genetic study was carried out by real-time polymerase chain reaction to determine the characteristic of the Russian population hereditary genetic variant c.470Т>С in the CHEK2 gene using a diagnostic panel that allows to determine three germline mutations: c.1100delC, c.444+1G>A and c.470Т>С in the CHEK2 gene.Results. In patients with BC the frequency of the mutation c.470T>C in the CHEK2 gene was 3.8 %, in patients with BBD this mutation was detected in 4.7 % of cases. The frequency of the genetic variant c.470T>C in high-risk groups was: 5.1 % – for BC patients with clinical signs of hereditary disease and 4.9 % – for patients with BBD with a family history of cancer. There were no statistically significant differences between the frequency of the mutation c.470T>C in the general groups of BC patients and patients with BBD and the corresponding frequency in the high-risk groups, as well as in the groups of BC patients and patients with BBD (p >0.05).Conclusion. The results of this study indicate the probable absence of a relationship between the presence of the mutation c.470Т>С in the CHEK2 gene and an increased risk of BC.
The effect of hypoxia on the secretome of human glioblastoma multiforme cells
Authors: Kushnir T. I. , Arnotskaya N. E. , Kudryavtsev I. A. , Mitrofanov A. A. , Bekyashev A. K. , Shevchenko V. E. 
Published: May 2021

Background. Glioblastoma multiforme (GBM) develops in the hypoxic microenvironment, which plays an important role in the pathogenesis of the disease and is closely associated with tumor growth, development and poor prognosis. Hypoxia increases the resistance of tumor cells (TC) to radiation therapy and chemotherapy, promotes the appearance of an aggressive TC phenotype, leading to the disease recurrence. The molecular mechanism of hypoxic action on the secretome of GBM cells, which is involved in the formation of the tumor microenvironment, remains unclear. Also, markers of the aggressive hypoxia-associated phenotype of tumor cells have not been established. The purpose of research – to study the molecular mechanisms of the hypoxia-associated effect on the secretome of the U251 GBM cells.Materials and method. High resolution proteomic mass spectrometry, cell technologies.Results. A total of 1432 proteins were identified in the secretomes of two types of GBM cells (control and experiment). After the action of hypoxia, statistically significant changes in the expression of 390 proteins were registered. 11 proteins showed increase in expression over two orders of magnitude. The intracellular signaling pathways which are responsible for the hypoxia-associated effects on the U251 GMB cells have been identified.Conclusions. Hypoxia significantly affected the proteomic composition of the GBM cells secretome. Five overexpressed secretome proteins, S100A6, HEY1, ZIP3, S100A4, ZEB2, have been proposed as potential markers of the hypoxiaassociated phenotype of GBM, for which participation in the pathogenesis of glioblastoma multiforme has been previously showed.