bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification and functional analysis of a three-miRNA ceRNA network in hypertrophic scars
Authors: Zhang Zewei , Huang Xin , Yang Jiahao , Gu Shuchen , Zhao Yixuan , Liu Yunhan , Khoong Yimin , Wang Shuqi , Luo Shenying , Zan Tao , Li Guangshuai 
Published: October 2021

Abstract
Background
Hypertrophic scar (HTS) is a fibrotic disorder of skins and may have repercussions on the appearance as well as functions of patients. Recent studies related have shown that competitive endogenous RNA (ceRNA) networks centering around miRNAs may play an influential role in HTS formation. This study aimed to construct and validate a three-miRNA (miR-422a, miR-2116-3p, and miR-3187-3p) ceRNA network, and explore its potential functions.

Methods
Quantitative real‑time PCR (qRT‑PCR) was used to compare expression levels of miRNAs, lncRNAs, and genes between HTS and normal skin. Target lncRNAs and genes of each miRNA were predicted using starBase as well as TargetScan database to construct a distinct ceRNA network; overlapping target lncRNAs and genes of the three miRNAs were utilized to develop a three-miRNA ceRNA network. For every network, protein–protein interaction (PPI) network analysis was performed to identify its hub genes. For each network and its hub genes, Gene Oncology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis were conducted to explore their possible functions.

Results
MiR-422a, miR-2116-3p, and miR-3187-3p were all downregulated in HTS tissues and fibroblasts. MiR-422a-based ceRNA network consisted of 101 lncRNAs with 133 genes; miR-2116-3p-centered ceRNA network comprised 85 lncRNAs and 978 genes; miR-3187-3p-derived ceRNA network encompassed 84 lncRNAs as well as 1128 genes. The three-miRNA ceRNA network included 2 lncRNAs with 9 genes, where MAPK1, FOSL2, ABI2, KPNA6, CBL, lncRNA-KCNQ1OT1, and lncRNA-EBLN3P were upregulated. According to GO and KEGG analysis, these networks were consistently related to ubiquitination. Three ubiquitination-related genes (CBL, SMURF2, and USP4) were upregulated and negatively correlated with the expression levels of the three miRNAs in HTS tissues.

Conclusions
This study identified a three-miRNA ceRNA network, which might take part in HTS formation and correlate with ubiquitination.
Integrins regulate stemness in solid tumor: an emerging therapeutic target
Authors: Xiong Jiangling , Yan Lianlian , Zou Cheng , Wang Kai , Chen Mengjie , Xu Bin , Zhou Zhipeng , Zhang Dingxiao 
Published: October 2021

AbstractIntegrins are the adhesion molecules and transmembrane receptors that consist of α and β subunits. After binding to extracellular matrix components, integrins trigger intracellular signaling and regulate a wide spectrum of cellular functions, including cell survival, proliferation, differentiation and migration. Since the pattern of integrins expression is a key determinant of cell behavior in response to microenvironmental cues, deregulation of integrins caused by various mechanisms has been causally linked to cancer development and progression in several solid tumor types. In this review, we discuss the integrin signalosome with a highlight of a few key pro-oncogenic pathways elicited by integrins, and uncover the mutational and transcriptomic landscape of integrin-encoding genes across human cancers. In addition, we focus on the integrin-mediated control of cancer stem cell and tumor stemness in general, such as tumor initiation, epithelial plasticity, organotropic metastasis and drug resistance. With insights into how integrins contribute to the stem-like functions, we now gain better understanding of the integrin signalosome, which will greatly assist novel therapeutic development and more precise clinical decisions.
Organizational skills training for children with ADHD: study protocol for a randomized, controlled trial
Authors: Bikic Aida , Dalsgaard Søren , Olsen Kristoffer Dalsgaard , Sukhodolsky Denis G. 
Published: October 2021

Abstract
Background
Problems with sustained attention, impulsivity, and hyperactivity are the most prominent symptoms of attention-deficit hyperactivity disorder (ADHD), but many children with this diagnosis also present with poor organizational skills that are important in relation to school. These problems tend to increase from childhood to adolescence and are often not well managed by medication. Organizational skills training (OST) is a range of behavioral interventions that specifically target organizational skills deficits. Evidence supports the effect of OST on improving organizational skills, inattention, and academic performance in children with ADHD. Because previous clinical trials included mostly children above the age of 8 years, this trial includes children in the age range 6–13 years to expand the knowledge on the effects of OST in younger children. Previous OST research has also shown improvement on inattention in parent ratings; we will investigate if a change in inattention can be confirmed with neurocognitive tests. Finally, little is known about predictors of treatment response in OST.

Objectives
The primary objective is to investigate if OST has positive effects on organizational skills in children with ADHD. The primary outcome measurement is the parent-rated Children’s Organizational Skills Scale (COSS), collected before and at the end of the 10 week intervention. Secondary and exploratory outcomes include inattention ratings, family and school functioning, and cognitive functions measured before the intervention period, immediately after, and at a 6 month follow-up. Additional objectives are to investigate both neurocognitive outcomes and age as predictors of treatment response.

Methods
This is a randomized clinical superiority trial investigating the effect of OST vs a treatment-as-usual (TAU) control group for children with ADHD in the age range of 6–13 years. All participants (n = 142) receive TAU. OST is administered in a group format of 10 weekly sessions. Adverse events are monitored by study clinicians during weekly therapy sessions and all assessments. Data analyses will be conducted using mixed linear regression models with random intercepts for patients, adjusted for the stratification variables and the baseline value.

Perspectives
This study will provide important new knowledge and expand on existing research in the field of non-pharmacological treatment of children with ADHD. OST can potentially have a significant impact on the lives of children with ADHD by helping them learn how to cope with their present deficits and to become more independent and self-reliant. It is also important to investigate predictors of treatment response in order to optimize OST.

Trial registration
ClinicalTrials.gov NCT03160378. Registered on May 19, 2017.
DNA methylation of the KLK8 gene in depression symptomatology
Authors: Starnawska Anna , Bukowski Lina , Chernomorchenko Ana , Elfving Betina , Müller Heidi Kaastrup , van den Oord Edwin , Aberg Karolina , Guintivano Jerry , Grove Jakob , Mors Ole , Børglum Anders D. , Nielsen Anders L. , Qvist Per , Staunstrup Nicklas Heine 
Published: October 2021

Abstract
Background
Depression is a common, complex, and debilitating mental disorder estimated to be under-diagnosed and insufficiently treated in society. Liability to depression is influenced by both genetic and environmental risk factors, which are both capable of impacting DNA methylation (DNAm). Accordingly, numerous studies have researched for DNAm signatures of this disorder. Recently, an epigenome-wide association study of monozygotic twins identified an association between DNAm status in the KLK8 (neuropsin) promoter region and severity of depression symptomatology.

Methods
In this study, we aimed to investigate: (i) if blood DNAm levels, quantified by pyrosequencing, at two CpG sites in the KLK8 promoter are associated with depression symptomatology and depression diagnosis in an independent clinical cohort and (ii) if KLK8 DNAm levels are associated with depression, postpartum depression, and depression symptomatology in four independent methylomic cohorts, with blood and brain DNAm quantified by either MBD-seq or 450 k methylation array.

Results
DNAm levels in KLK8 were not significantly different between depression cases and controls, and were not significantly associated with any of the depression symptomatology scores after correction for multiple testing (minimum p value for KLK8 CpG1 = 0.12 for ‘Depressed mood,’ and for CpG2 = 0.03 for ‘Loss of self-confidence with other people’). However, investigation of the link between KLK8 promoter DNAm levels and depression-related phenotypes collected from four methylomic cohorts identified significant association (p value < 0.05) between severity of depression symptomatology and blood DNAm levels at seven CpG sites.

Conclusions
Our findings suggest that variance in blood DNAm levels in KLK8 promoter region is associated with severity of depression symptoms, but not depression diagnosis.
H3K36 trimethylation-mediated biological functions in cancer
Authors: Xiao Chu , Fan Tao , Tian He , Zheng Yujia , Zhou Zheng , Li Shuofeng , Li Chunxiang , He Jie 
Published: October 2021

AbstractHistone modification is an important form of epigenetic regulation. Thereinto, histone methylation is a critical determination of chromatin states, participating in multiple cellular processes. As a conserved histone methylation mark, histone 3 lysine 36 trimethylation (H3K36me3) can mediate multiple transcriptional-related events, such as the regulation of transcriptional activity, transcription elongation, pre-mRNA alternative splicing, and RNA m6A methylation. Additionally, H3K36me3 also contributes to DNA damage repair. Given the crucial function of H3K36me3 in genome regulation, the roles of H3K36me3 and its sole methyltransferase SETD2 in pathogenesis, especially malignancies, have been emphasized in many studies, and it is conceivable that disruption of histone methylation regulatory network composed of “writer”, “eraser”, “reader”, and the mutation of H3K36me3 codes have the capacity of powerfully modulating cancer initiation and development. Here we review H3K36me3-mediated biological processes and summarize the latest findings regarding its role in cancers. We highlight the significance of epigenetic combination therapies in cancers.
Valproic acid promotes the in vitro differentiation of human pluripotent stem cells into spermatogonial stem cell-like cells
Authors: Wang Xiaotong , Qu Mengyuan , Li Zili , Long Yuting , Hong Kai , Li Honggang 
Published: October 2021

Abstract
Background
Studying human germ cell development and male infertility is heavily relied on mouse models. In vitro differentiation of human pluripotent stem cells into spermatogonial stem cell-like cells (SSCLCs) can be used as a model to study human germ cells and infertility. The current study aimed to develop the SSCLC induction protocol and assess the effects of the developed protocol on SSCLC induction.

Methods
We examined the effects of valproic acid (VPA), vitamin C (VC) and the combination of VPA and VC on the SSCLC induction efficiency and determined the expression of spermatogonial genes of differentiated cells. Haploid cells and cells expressed meiotic genes were also detected. RNA-seq analysis was performed to compare the transcriptome between cells at 0 and 12 days of differentiation and differently expressed genes were confirmed by RT-qPCR. We further evaluated the alteration in histone marks (H3K9ac and H3K27me3) at 12 days of differentiation. Moreover, the SSCLC induction efficiency of two hiPSC lines of non-obstructive azoospermia (NOA) patients was assessed using different induction protocols.

Results
The combination of low concentrations of VPA and VC in the induction medium was most effective to induce SSCLCs expressing several spermatogonial genes from human pluripotent stem cells at 12 days of differentiation. The high concentration of VPA was more effective to induce cells expressing meiotic genes and haploid cells. RNA-seq analysis revealed that the induction of SSCLC involved the upregulated genes in Wnt signaling pathway, and cells at 12 days of differentiation showed increased H3K9ac and decreased H3K27me3. Additionally, two hiPSC lines of NOA patients showed low SSCLC induction efficiency and decreased expression of genes in Wnt signaling pathway.

Conclusions
VPA robustly promoted the differentiation of human pluripotent stem cells into SSCLCs, which involved the upregulated genes in Wnt signaling pathway and epigenetic changes. hiPSCs from NOA patients showed decreased SSCLC induction efficiency and Wnt signaling pathway gene expression, suggesting that SSC depletion in azoospermia testes might be associated with inactivation of Wnt signaling pathway. Our developed SSCLC induction protocol provides a reliable tool and model to study human germ cell development and male infertility.
Lessons from Israel's COVID-19 Green Pass program
Authors: Kamin-Friedman Shelly , Peled Raz Maya 
Published: October 2021

AbstractAs of the beginning of March 2021, Israeli law requires the presentation of a Green Pass as a precondition for entering certain businesses and public spheres. Entitlement for a Green Pass is granted to Israelis who have been vaccinated with two doses of COVID-19 vaccine, who have recovered from COVID-19, or who are participating in a clinical trial for vaccine development in Israel. The Green Pass is essential for retaining immune individuals' freedom of movement and for promoting the public interest in reopening the economic, educational, and cultural spheres of activity. Nonetheless, and as the Green Pass imposes restrictions on the movement of individuals who had not been vaccinated or who had not recovered, it is not consonant with solidarity and trust building. Implementing the Green Pass provision while advancing its effectiveness on the one hand, and safeguarding equality, proportionality, and fairness on the other hand may imbue this measure with ethical legitimacy despite involving a potential breach of trust and solidarity.
Duplication elimination in cache-uplink transmission over B5G small cell network
Authors: Sufyan Mubarak Mohammed Al-Ezzi , Rehman Waheed Ur , Salam Tabinda , Ali Qazi Ejaz , Ilyas Abeera , Quradaa Fahmi 
Published: October 2021

AbstractIn this era of the digital world, data play a central role and are continuously challenging spectrum efficiency. With the introduction of enriched multimedia user-generated content, the challenges are even more aggravated. In this vein, uplink caching is considered as one of the promising solutions to effectively cater the user’s demands. One of the main challenges for uplink caching is duplication elimination. In this paper, a cache enabled uplink transmission with a duplication elimination scheme is proposed. The proposed scheme matches the mobile’s data to be uploaded with the cached contents both at mobile station (MS) and small base station (SBS). In contrast to existing techniques, the proposed scheme broadcasts the cached contents at an SBS to all the MSs under its footprint. This provides MS an opportunity to exploit the list of cached contents before uploading its data. A MS only uploads its data if it is not already cached at an SBS. This significantly reduces duplication before the real transmission takes place. Furthermore, the proposed technique reduces energy consumption in addition to improving spectral efficiency and network throughput. Besides, a higher caching hit ratio and lower caching miss ratio are also observed as compared to other schemes. The simulation results reveal that the proposed scheme saves 97% energy for SBS, whereas 96–100% energy is saved for MS on average.
Simulation of urban microclimate with uhiSolver: software validation using simplified material data
Authors: Teichmann Florian , Baumgartner Christina Maria , Horvath Andras , Luisser Markus , Korjenic Azra 
Published: October 2021

Abstract
Background
Increasing urbanization as well as global warming requires an investigation of the influence of different construction methods and ground surfaces on the urban heat island effect (UHI effect). The extent of the influence of the urban structure, the building materials used and their surfaces on the UHI effect can be significantly reduced already in the planning phase using a designated OpenFOAM-based solver “uhiSolver”.

Results
In the first part of this research work, it is shown that inner building details and components can be neglected while still obtaining sufficiently accurate results. For this purpose, the building model was divided into two layers: a surface layer without mass, where the interaction with radiation takes place, and a component layer, which contains all relevant components and cavities of the building represented with mass-averaged material properties. It has become apparent that the three parameters—albedo, heat capacity and thermal resistance—which have a decisive influence on the interaction, have different effects on the component temperatures and the surface temperatures. In the second part of this research work, dynamic 3D computational fluid dynamics (CFD) simulations are performed with uhiSolver for a residential block in Vienna. Comparing the simulation results with measurement data collected on site, it is shown that the simplified assumption of homogeneous material data for building bodies provides very good results for the validation case investigated. However, the influence of the greening measures in the courtyard of the residential block on the air temperature is found to be negligible. Furthermore, it was observed that due to locally higher radiation density, lower air velocities and higher air humidity, the apparent temperature in the courtyard is sometimes perceived to be higher than in the adjacent streets, despite the lower air temperature.

Conclusions
Simplifying the modeling process of the uhiSolver software by reducing the model complexity helps to reduce manual work for setting up appropriate boundary conditions of buildings. Compared to market competitors, good results are obtained for the validation case Kandlgasse presented in this research work, despite the simplifications proposed. Thus, uhiSolver can be used as a robust analytical tool for urban planning.
The effect of mood on food versus non-food interference among females who are high and low on emotional eating
Authors: Sambal Hilla , Bohon Cara , Weinbach Noam 
Published: October 2021

Abstract
Background
Emotional eating refers to overeating triggered by emotional experiences and may cause significant psychological distress and health problems. Thus, it is important to better understand its underlying mechanisms. The study examined if the ability to ignore task-irrelevant information, namely, interference control, is modulated by mood and exposure to food stimuli among females who are high and low on emotional eating.

Method
The study’s sample included 80 women who were high (N = 40) or low (N = 40) on an emotional eating scale. Participants were divided to a negative or neutral mood induction group. Following the mood induction, they completed a food-flanker task that allowed assessing attentional interference caused by food and non-food stimuli separately.

Results
The low emotional eating group had significantly greater food compared to non-food interference, suggesting difficulty at ignoring food stimuli while attending a neutral target. In the high emotional eating group, there was no difference between food and non-food interference. However, higher levels of emotional eating predicted lower levels of food interference.

Conclusion
The pattern of results suggests a food-avoidance attentional tendency among those with higher levels of emotional eating. The mood manipulation did not influence food-related interference in either group. The lack of an effect of mood on food-related interference questions the impact of negative emotions on basic attentional processes among individuals with emotional eating.
An empirical study of Chinese students' behavioral intentions to adopt 5G for smart-learning in Covid-19
Authors: Shah Sayed Kifayat , Tang Zhongjun , Sharif Sayed Muhammad Fawad , Tanveer Arifa 
Published: October 2021

AbstractThe social distancing due to the Covid-19 epidemic has disturbed all sectors of society, including education. To maintain normal operations, it is necessary to adapt quickly to this situation. Many technologies and platforms have rushed to offer their support to users. This article adopts a critical perspective to reflect on the factors that may cause the hasty adoption of 5G smart learning technology. To investigate students' intentions toward smart learning, this article provides a theoretical framework premised on the technology acceptance model (TAM) by adding components from the social practise theory (SPT). Based on data analysis through Structural equation Modeling (SEM) of a survey (n = 375) conducted in China, we found that the choice of 5G smart-learning technology depends on the combined effect of Material (MAA), Meanings (MEA), and Competency access (COA) factors. The results illustrate that these are the effective factors for student’s intentions to adopt 5G smart-learning technology. These outcomes are intended to aid service providers and decision-makers in developing effective ways to increase smart learning use. These findings can also enable us to identify challenges affecting smart learning adoption and to contribute to the design and proper supply of smart learning programs in other countries.
Involving stakeholders in research priority setting: a scoping review
Authors: Grill Christiane 
Published: October 2021

Abstract
Background
This scoping review provides a thorough analysis of how stakeholders have so far been involved in research priority setting. The review describes, synthesizes, and evaluates research priority setting projects not only for the field of health—as previous reviews have done—but does so on a much broader scale for any research area.

Methods
A comprehensive electronic literature search was conducted in the databases PubMed, Scopus, and Web of Science. Reflecting the importance of grey literature, Google Scholar and relevant websites were also screened for eligible publications. A computational approach was then used for the study selection. The final screening for inclusion was done manually.

Results
The scoping review encompasses 731 research priority setting projects published until the end of 2020. Overall, the projects were conducted within the realm of 50 subject areas ranging from agriculture and environment over health to social work and technology. Key learnings include that nearly all priority setting projects aimed to identify research priorities for the field of health (93%), particularly for nursing and care, cancer, pediatrics, and mental, behavioral and neurodevelopmental disorders. Only 6% of the projects were not health-related and 1% identified research priorities at the interface between health and a non-health area. Over time, 30 different stakeholder groups took part in research priority setting. The stakeholders most frequently asked to identify research priorities were doctors, patients, academics/researchers, nurses, allied healthcare professionals, family members, friends, and carers. Nearly two thirds of all projects have been conducted in Europe and North America. Overall, only 9% of the projects emphasized the importance of stakeholders in their goals and rationales and actively involved them. In around a quarter of the projects, stakeholders deliberated on their research priorities throughout the entire process.

Conclusion
By mapping out the complex landscape of stakeholder involvement in research priority setting, this review guides future efforts to involve stakeholders effectively, inclusively, and transparently, which in turn may increase the overall value of research for society. As a practical addition to this review, the first worldwide research priority setting database was created: https://ois.lbg.ac.at/en/project-database. The database contains all the projects analyzed for this review and is constantly updated with the latest published research priority setting projects.