bluuebunny/crossref_metadata_2025_split huggingface dataset:
Kesiapan Individu Dan Organisasi Terhadap Komitmen Afektif Untuk Berubah di Masa Pandemic Covid-19
Authors: Angesti Dyan , Muhadi Muhadi , Prameswari Syanindita 
Published: November 2021

STIKES YRSDS sebagai salah satu perguruan tinggi swasta yang berkembang, kondisi pandemi COVID-19 ini merupakan hantaman luar biasa dan menjadi penentu apakah STIKES YRSDS mampu bertahan atau akan terhempas di era persaingan. Perubahan cepat telah dilakukan salah satunya adalah perubahan struktur organisasi. Namun perubahan ini bukan hal yang mudah, hasil evaluasi semester genap TA 2019-2020 pembelajaran daring dan layanan di STIKES YRSDS sebesar <80% sedangkan standar kepuasan >80%. Kepuasan mahasiswa kurang dari SPMI STIKES YRSDS. Perubahan ini secara langsung akan berdampak pada mekanisme tata kelola yang ada. Tujuan penelitian yaitu menganalisis pengaruh kesiapan individu dan organisasi STIKES YRSDS terhadap komitmen afektif untuk berubah. Metode penelitian ini menggunakan teknik non-probability sampling dengan metode convenience sampling. Subjek penelitian yaitu seluruh Karyawan yang sudah menjadi tenaga tetap STIKES YRSDS sebanyak 44 orang. Hasil penelitian menunjukan Kesiapan Individu tidak berpengaruh signifikan terhadap komitmen afektif, uji variabel lain dalam penelitian menunjukkan bahwa Kesiapan Organisasi berpengaruh signifikan terhadap komitmen afektif, penelitian juga menunjukan Kesiapan Organisasi berpengaruh signifikan terhadap kinerja komitmen afektif. Hal ini menunjukan Kesiapan Organisasi memberikan pengaruh yang signifikasi terhadap komitmen afektif karyawan STIKES YRSDS. Penelitian juga menunjukkan Kesiapan Individu dan Kesiapan Organisasi berpengaruh signifikan terhadap komitmen afektif karyawan STIKES YRSDS. Kesimpulan Kesiapan individu karyawan tergolong tinggi dalam menghadapi perubahan di masa pandemic covid19, Kesiapan organisasi tergolong sedang dalam menghadapi perubahan di masa pandemic covid19, ada pengaruh kesiapan organisasi terhadap komitmen afektif karyawan di masa pandemic covid19 dan tidak ada pengaruh antara kesiapan individu terhadap komitmen afektif karyawan STIKES YRSDS di masa pandemic covid19.
Neurogenic dysfunction of the lower urinary tract (neurogenic bladder)
Authors: Sorokin Yu. N. 
Published: November 2021

The lecture presents information about the central and peripheral structures that provide innervation and regulation of the functions of the lower urinary tract — the bladder and urethra. The mechanisms of regulation of the functions of accumulation and retention of urine (reservoir function) and emptying of the bladder (evacuation function) are shown.Neural control of urination is organized in the form of a hierarchical system. The spinal centers are the executive structures, and the cerebral centers are the controlling structures. Involuntary implementation of the act of urination occurs through segmental sympathetic and parasympathetic mechanisms. The regulation of these functions and voluntary urination are carried out by the central mechanisms of the brain — the actuation of the urination reflex is under strict volitional control, which makes it possible to plan the emptying of the bladder in a socially acceptable place and time.The information on the clinical symptoms of neurogenic dysfunction of the lower urinary tract (neurogenic bladder) is reviewed. The features of clinical manifestations depending on the level of damage to neural structures and in various neurological diseases are shown. Methods for assessing urodynamics and treatment directions for neurogenic dysfunction of the lower urinary tract are presented.
Cerebral autosomo-dominant arteriopathy with subcortical infarctions and leukoencephalopathy
Authors: Savchuk E. A. , Golubinskaya E. P. , Shcherbinina T. N. , Voronin G. Yu. , Savchuk E. O. , Zyablitskaya E. Yu., , Makalish T. P. 
Published: November 2021

The article presents an analysis of the literature and a clinical case of a rare disease from the group of diseases of small vessels — cerebral autosomal dominant arteriopathy with subcortical infarction and leukoencephalopathy (CADASIL).It is based on the deposition of osmiophilic granulation material in vessels of small and medium caliber. A mutation in the NOTCH3 gene on chromosome 19p13 leads to significant structural changes in the walls of small arteries due to impaired diﬀerentiation and maturation of smooth muscle cells.CADASIL is characterized by four key symptoms: migraines, recurrent ischemic strokes, mental disorders, and cognitive decline. The clinical case study is presented from the standpoint of a multidisciplinary patient-oriented approach of joint work of neurologists and morphologists. On the basis of clinical and laboratory criteria, a probable diagnosis was made. To confirm it, a muscle biopsy was performed (a musculocutaneous flap from the inner surface of the thighs and forearms), in order to conduct light and electron microscopy. The details of the results of the morphological study, which made it possible to verify the patient’s diagnosis, are presented. Diﬀerential diagnostic judgments are presented and recommendations for genetic studies in the family, prognosis and treatment of the patient are given.
Primeiro a vida!
Authors: Abi-Saab Javier Augusto 
Published: November 2021

Desde a antropologia ontológica, este artigo apresenta como a pandemia COVID-19 se experimenta como uma oportunidade para a comunidade Avié de nacionalidade ai’cofán da Amazônia Equatoriana, para o fortalecimento do território através das práticas agroecológicas que retomam valores tradicionais e ações de relacionalidade com a selva. A agroecologia, enmarcada no contexto da mutação ecológica, constitui uma resposta ao novo coronavírus ao estabelecer práticas que cuidam da nossa saúde, dos nossos corpos e do nosso alimento, temas que estão no centro dos impactos da pandemia. COVID-19 emerge para a comunidade Avié e para os coletivos ecologistas como um sintoma do desequilíbrio e desconexão do mundo urbano e industrial moderno com os seres da natureza. Ao longo do texto se analisa por que as práticas e valores agroecológicos relacionados à vida são uma resposta contundente e sistêmica à pandemia e à mutação ecológica que experimentamos.
Iron Oxide–Modified Carbon Electrode and Sulfate-Reducing Bacteria for Simultaneous Enhanced Electricity Generation and Tannery Wastewater Treatment
Authors: Miran Faiz , Mumtaz Muhammad Waseem , Mukhtar Hamid , Akram Sadia 
Published: November 2021

The microbial fuel cell (MFC) is emerging as a potential technology for extracting energy from wastes/wastewater while they are treated. The major hindrance in MFC commercialization is lower power generation due to the sluggish transfer of electrons from the biocatalyst (bacteria) to the anode surface and inefficient microbial consortia for treating real complex wastewater. To overcome these concerns, a traditional carbon felt (CF) electrode modification was carried out by iron oxide (Fe3O4) nanoparticles via facile dip-and-dry methods, and mixed sulfate-reducing bacteria (SRBs) were utilized as efficient microbial consortia. In the modified CF electrode with SRBs, a considerable improvement in the bioelectrochemical operation was observed, where the power density (309 ± 13 mW/m2) was 1.86 times higher than bare CF with SRBs (166 ± 11 mW/m2), suggesting better bioelectrochemical performance of an SRB-enriched Fe3O4@CF anode in the MFC. This superior activity can be assigned to the lower charge transfer resistance, higher conductance, and increased number of catalytic sites of the Fe3O4@CF electrode. The SRB-enriched Fe3O4@CF anode also assists in enhancing MFC performance in terms of COD removal (&gt;75%), indicating efficient biodegradability of tannery wastewater and a higher electron transfer rate from SRBs to the conductive anode. These findings demonstrate that a combination of the favorable properties of nanocomposites such as Fe3O4@CF anodes and efficient microbes for treating complex wastes can encourage new directions for renewable energy–related applications.
Costunolide-Induced Apoptosis via Promoting the Reactive Oxygen Species and Inhibiting AKT/GSK3β Pathway and Activating Autophagy in Gastric Cancer
Authors: Xu Cuixiang , Huang Xiaoyan , Lei Xiaohua , Jin Zhankui , Wu Min , Liu Xiao , Huang Yubin , Zhao Xiangrong , Xiong Yue , Sun Jingying , Duan Xianglong , Wang Jianhua 
Published: November 2021

Objective: Costunolide (Cos) is a sesquiterpene lactone extracted from chicory. Although it possesses anti-tumor effects, the underlying molecular mechanism against gastric cancer cells remains unclear. This study aimed to explore the effect and potential mechanism of Cos on gastric cancer.Methods: The effect of Cos on HGC-27 and SNU-1 proliferation was detected by CCK-8 and clone formation assay. The changes in cell apoptosis were determined using Hoechst 33258 and tunel staining. The morphology of autophagy was analyzed by autophagosomes with the electron microscope and LC3-immunofluorescence with the confocal microscope. The related protein levels of the cell cycle, apoptosis, autophagy and AKT/GSK3β pathway were determined by Western blot. The anti-tumor activity of Cos was evaluated by subcutaneously xenotransplanting HGC-27 into Balb/c nude mice. The Ki67 and P-AKT levels were examined by immunohistochemistry.Results: Cos significantly inhibited HGC-27 and SNU-1 growth and induced cell cycle arrest in the G2/M phase. Cos activated intrinsic apoptosis and autophagy through promoting cellular reactive oxygen species (ROS) levels and inhibiting the ROS-AKT/GSK3β signaling pathway. Moreover, preincubating gastric carcinoma cells with 3-methyladenine (3-MA), a cell-autophagy inhibitor, significantly alleviated the effects of Cos in inducing cell apoptosis.Conclusion: Cos induced apoptosis of gastric carcinoma cells via promoting ROS and inhibiting AKT/GSK3β pathway and activating pro-death cell autophagy, which may be an effective strategy to treat gastric cancer.
Deconvolution of Bulk Gene Expression Profiles with Single-Cell Transcriptomics to Develop a Cell Type Composition-Based Prognostic Model for Acute Myeloid Leukemia
Authors: Dai Chengguqiu , Chen Mengya , Wang Chaolong , Hao Xingjie 
Published: November 2021

Acute myeloid leukemia (AML) is one of the malignant hematologic cancers with rapid progress and poor prognosis. Most AML prognostic stratifications focused on genetic abnormalities. However, none of them was established based on the cell type compositions (CTCs) of peripheral blood or bone marrow aspirates from patients at diagnosis. Here we sought to develop a novel prognostic model for AML in adults based on the CTCs. First, we applied the CIBERSORT algorithm to estimate the CTCs for patients from two public datasets (GSE6891 and TCGA-LAML) using a custom gene expression signature reference constructed by an AML single-cell RNA sequencing dataset (GSE116256). Then, a CTC-based prognostic model was established using least absolute shrinkage and selection operator Cox regression, termed CTC score. The constructed prognostic model CTC score comprised 3 cell types, GMP-like, HSC-like, and T. Compared with the low-CTC-score group, the high-CTC-score group showed a 1.57-fold [95% confidence interval (CI), 1.23 to 2.00; p = 0.0002] and a 2.32-fold (95% CI, 1.53 to 3.51; p &lt; 0.0001) higher overall mortality risk in the training set (GSE6891) and validation set (TCGA-LAML), respectively. When adjusting for age at diagnosis, cytogenetic risk, and karyotype, the CTC score remained statistically significant in both the training set [hazard ratio (HR) = 2.25; 95% CI, 1.20 to 4.24; p = 0.0119] and the validation set (HR = 7.97; 95% CI, 2.95 to 21.56; p &lt; 0.0001]. We further compared the performance of the CTC score with two gene expression-based prognostic scores: the 17-gene leukemic stem cell score (LSC17 score) and the AML prognostic score (APS). It turned out that the CTC score achieved comparable performance at 1-, 2-, 3-, and 5-years timepoints and provided independent and additional prognostic information different from the LSC17 score and APS. In conclusion, the CTC score could serve as a powerful prognostic marker for AML and has great potential to assist clinicians to formulate individualized treatment plans.
The Effect of Exogenous Sex Steroids on the Vaginal Microbiota: A Systematic Review
Authors: Ratten Larissa K. , Plummer Erica L. , Bradshaw Catriona S. , Fairley Christopher K. , Murray Gerald L. , Garland Suzanne M. , Bateson Deborah , Tachedjian Gilda , Masson Lindi , Vodstrcil Lenka A. 
Published: November 2021

BackgroundExogenous sex steroids within hormonal contraception and menopausal hormone therapy (MHT) have been used for family planning and management of menopausal symptoms, without consideration of their effects on the vaginal microbiota. This is largely because their use predates our understanding of the importance of the vaginal microbiome on human health. We conducted a systematic review (PROSPERO: CRD42018107730) to determine the influence of exogenous sex steroids, stratified by oestrogen-containing or progestin-only types of contraception, and MHT on the vaginal microbiome, as measured by molecular methods.MethodsEmbase, PubMed and Medline were searched for relevant literature published through to December 1st 2020. Eligible studies reported on the effect of specific exogenous sex steroids on the vaginal microbiome using a molecular method. Data regarding the ‘positive’, ‘negative’ or ‘neutral’ effect of each type of contraceptive or MHT on the vaginal microbiome was extracted and summarised. A positive effect reflected sex steroid exposure that was associated with increased abundance of lactobacilli, a change to, or maintenance of, an optimal vaginal microbiota composition, or a decrease in bacterial diversity (specifically reflecting a low-diversity optimal microbiota state), relative to the control group. An exogenous sex steroid was designated as having a negative effect on the vaginal microbiome if it resulted in opposing effects (i.e. loss of lactobacilli, a non-optimal microbiota state). When no significant change was found, this was considered neutral/inconclusive.ResultsWe identified 29 manuscripts reporting on the effect of exogenous sex steroids on the vaginal microbiome; 25 investigating hormonal contraceptives, and 4 investigating MHT. Oestrogen-containing contraception, particularly reflecting the combined oestrogen and progestin-containing contraceptive pill, had a positive effect on the composition of the vaginal microbiota. Progestin-only contraception, particularly reflecting depo-medroxyprogesterone acetate, had mixed effects on the microbiota. Among post-menopausal women using MHT, exogenous oestrogen applied topically was associated with increased prevalence of lactobacilli.ConclusionOur findings suggest that oestrogen-containing compounds may promote an optimal vaginal microbiota, which could have clinical applications. The impact of progestin-only contraceptives on the vaginal microbiota is less clear; more data is needed to determine how progestin-only contraceptives contribute to adverse reproductive and sexual health outcomes.
Characterization of Naturally Acquired Immunity to a Panel of Antigens Expressed in Mature P. falciparum Gametocytes
Authors: Muthui Michelle K. , Takashima Eizo , Omondi Brian R. , Kinya Christine , Muasya William I. , Nagaoka Hikaru , Mwai Kennedy W. , Orindi Benedict , Wambua Juliana , Bousema Teun , Drakeley Chris , Blagborough Andrew M. , Marsh Kevin , Bejon Philip , Kapulu Melissa C. 
Published: November 2021

IntroductionNaturally acquired immune responses against antigens expressed on the surface of mature gametocytes develop in individuals living in malaria-endemic areas. Evidence suggests that such anti-gametocyte immunity can block the development of the parasite in the mosquito, thus playing a role in interrupting transmission. A better comprehension of naturally acquired immunity to these gametocyte antigens can aid the development of transmission-blocking vaccines and improve our understanding of the human infectious reservoir.MethodsAntigens expressed on the surface of mature gametocytes that had not previously been widely studied for evidence of naturally acquired immunity were identified for protein expression alongside Pfs230-C using either the mammalian HEK293E or the wheat germ cell-free expression systems. Where there was sequence variation in the candidate antigens (3D7 vs a clinical isolate PfKE04), both variants were expressed. ELISA was used to assess antibody responses against these antigens, as well as against crude stage V gametocyte extract (GE) and AMA1 using archived plasma samples from individuals recruited to participate in malaria cohort studies. We analyzed antibody levels (estimated from optical density units using a standardized ELISA) and seroprevalence (defined as antibody levels greater than three standard deviations above the mean levels of a pool of malaria naïve sera). We described the dynamics of antibody responses to these antigens by identifying factors predictive of antibody levels using linear regression models.ResultsOf the 25 antigens selected, seven antigens were produced successfully as recombinant proteins, with one variant antigen, giving a total of eight proteins for evaluation. Antibodies to the candidate antigens were detectable in the study population (N = 216), with seroprevalence ranging from 37.0% (95% CI: 30.6%, 43.9%) for PSOP1 to 77.8% (95% CI: 71.6%, 83.1%) for G377 (3D7 variant). Responses to AMA1 and GE were more prevalent than those to the gametocyte proteins at 87.9% (95% CI: 82.8%, 91.9%) and 88.3% (95% CI: 83.1%, 92.4%), respectively. Additionally, both antibody levels and breadth of antibody responses were associated with age and concurrent parasitaemia.ConclusionAge and concurrent parasitaemia remain important determinants of naturally acquired immunity to gametocyte antigens. Furthermore, we identify novel candidates for transmission-blocking activity evaluation.
Characteristics and Long-Term Ablation Outcomes of Supraventricular Arrhythmias in Hypertrophic Cardiomyopathy: A 10-Year, Single-Center Experience
Authors: Zhang Hong-Da , Ding Lei , Weng Si-Xian , Zhou Bin , Ding Xiao-Tong , Hu Li-Xing , Qi Ying-Jie , Yu Feng-Yuan , Feng Tian-Jie , Zhang Jing-Tao , Fang Pi-Hua , Hua Wei , Zhang Shu , Tang Min 
Published: November 2021

Background: A variety of supraventricular arrhythmias (SVAs) may occur in patients with hypertrophic cardiomyopathy (HCM). The characteristics and long-term ablation outcomes of different types of SVAs in HCM have not been comprehensively investigated.Methods: We retrospectively enrolled 101 consecutive patients with HCM who were referred to the electrophysiology and arrhythmia service from May 2010 to October 2020. The clinical features and ablation outcomes were analyzed.Results: Seventy-eight patients had SVAs, which comprised 50 (64.1%) cases of atrial fibrillation (AF), 16 (20.5%) of atrial flutter (AFL), 15 (19.2%) of atrioventricular reentrant tachycardia (AVRT), 11 (14.1%) of atrial arrhythmia (AT), and 3 (3.8%) of atrioventricular nodal reentrant tachycardia (AVNRT). Thirty-four patients underwent catheter ablation and were followed up for a median (interquartile range) of 58.5 (82.9) months. There was no recurrence in patients with non-AF SVAs. In patients with AF, the 1- and 7-year AF-free survival rates were 87.5 and 49.5%, respectively. A receiver operator characteristic analysis showed that a greater left ventricular end-diastolic dimension (LVEDD) was associated with a higher recurrence of AF, with an optimum cutoff value of 47 mm (c-statistic = 0.91, p = 0.011, sensitivity = 1.00, specificity = 0.82). In Kaplan–Meier analysis, patients with a LVEDD ≥ 47 mm had worse AF-free survival than those with a LVEDD &lt;47 mm (log-rank p = 0.014).Conclusions: In this unique population of HCM, AF was the most common SVA, followed in order by AFL, AVRT, AT, and AVNRT. The long-term catheter ablation outcome for non-AF SVAs in HCM is satisfactory. A greater LVEDD predicts AF recurrence after catheter ablation in patients with HCM.
Angiotensin-Converting Enzyme Inhibitors/Angiotensin Receptor Blockers: Anti-arrhythmic Drug for Arrhythmogenic Right Ventricular Cardiomyopathy
Authors: Tu Bin , Wu Lingmin , Zheng Lihui , Liu Shangyu , Sheng Lishui , Liu Limin , Zhu Zhenghui , Yao Yan 
Published: November 2021

Background: Current treatment guidelines for arrhythmogenic right ventricular cardiomyopathy (ARVC) mainly emphasize on prevention of ventricular arrhythmic events. Despite the progressive nature of ARVC, therapeutic options focusing on decelerating disease progression are scarce.Methods and Results: This retrospective observational cohort study included 311 patients [age, 39.1 ± 14.4 years; male, 233 (74.9%)] with a definite diagnosis of ARVC as determined by the 2010 Task Force Diagnostic Criteria. Among them, 113 patients (36.3%) received ACEI/ARB treatment. Disease progression was evaluated according to repeat transthoracic echocardiograms with a linear mixed model. Patients receiving ACEI/ARB treatment were associated with slower disease progression reflected by a gradual decrease in tricuspid annular plane systolic excursion than those not receiving ACEI/ARB treatment (0.37 vs. 0.61 mm per year decrease, P &lt; 0.001) and slower dilation of right ventricular outflow tract (0.57 vs. 1.06 mm per year increased, P = 0.003). Cox proportional hazard regression models were used to evaluate the association between life-threatening ventricular tachycardia events and ACEI/ARB treatment. A reduced risk of life-threatening ventricular arrhythmia was associated with ACEI/ARB treatment compared to that without ACEI/ARB treatment (adjusted HR: 0.71, 95% CI: 0.52–0.96, P = 0.031).Conclusions: ACEI/ARB treatment is associated with slower disease progression and lower risk of life-threatening ventricular arrhythmia in patients with ARVC. Delaying disease progression may pave way for reducing life-threatening ventricular arrhythmia risk.
Mapping Relation of Leakage Currents of Polluted Insulators and Discharge Arc Area
Authors: Fang Chunhua , Tao Yuning , Wang Jianguo , Ding Can , Huang Li , Zhou Mi , Gu Yi , Wang Yali 
Published: November 2021

A fundamental parameter of polluted insulator online monitoring is the leakage current, which has already been shown to be well-related to the pollution discharge of insulators. In this article, in an effort to quantitatively reflect the discharge intensity and the discharge status by the leakage current, we carried out an experimental study on artificial pollution discharge of insulators. A high-speed photographic apparatus was utilized to capture the entire process of local arcs on a porcelain insulator surface, including the arc generation, the arc development, and the flashover, for which the associated leakage current of insulators was synchronously digitized. A comparative analysis of the relation between the two-dimensional discharge image and the leakage current waveform in the process of arc generation and development shows that if the arc area on the insulator surface is relatively small and the leakage current passes through zero, the arc might completely become extinct, whereas this phenomena will not occur if the arc area is larger. In addition, the amplitude of the discharge arc area is found to be roughly proportional to the square of leakage current over the range of leakage current amplitude from 0 to 150 mA. Our results can provide an important guidance for judgment of the discharge status and the discharge intensity on insulator surfaces using the leakage current of insulators.