bluuebunny/crossref_metadata_2025_split huggingface dataset:
Development and Validation of a Robust Ferroptosis-Related Prognostic Signature to Predict Overall Survival in Clear Cell Renal Cell Carcinoma
Authors: Lin Yen-ting , Li Can-Xuan , Chen Jie 
Published: February 2021

Abstract
Background: Ferroptosis is a novel defined type of programmed cell death (PCD) with widespread functions involved in physical conditions or multiple diseases including malignancies. However, the relationship between ccRCC and ferroptosis-related regulators remains poorly known. Herein, we investigate the prognostic values and potential mechanisms of ferroptosis-related genes (FRGs) in ccRCC.Methods: Ferroptosis-related genes were obtained from FerrDb database, GeneCards database and previously published literatures. The gene expression profile of ferroptosis-related regulators and corresponding clinicopathological information were downloaded from The Cancer Genome Atlas (TCGA). Differentially expressed ferroptosis-related genes (DE-FRGs) were screened between ccRCC specimens and noncancerous specimens. Among these genes, prognostic DE-FRGs were identified using univariate COX analysis and LASSO regression analysis. Further multivariate COX regression was employed to identify prognosis-related hub DE-FRGs and establish a prognostic model. Results: We identified seven hub genes (HMGCR, MT1G, BID, EIF4A1, FOXM1, TFAP2C and CHAC1) from the DE-FRGs using univariate Cox regression analysis, LASSO and multivariate Cox regression analysis, and used them to establish a novel clinical predictive model in the TCGA train cohort (n = 374). Subsequently, we assessed the prognostic value of the model. Survival analysis showed that high-risk patients had a reduced overall survival (OS), the time-dependent receiver operating characteristic (ROC) curve analysis confirmed the signature's diagnostic performance. Additionally, multivariate Cox regression analysis suggested that the risk score was an independent prognostic factor. Additionally, we verified the prognostic performance of the risk model in the testing cohort (n=156), and the entire group (n=530) using Kaplan-Meier curve and ROC curve analyses. Functional analysis indicated that several carcinogenic pathways were enriched, and tumor-infiltrating immune cell abundances, and the expression levels of immunosuppressive molecules were different between two risk groups. Finally, external databases (ONCMINE, GEPIA, HPA, Kaplan-Meier plotter and cbioportal) were used to confirm the expression patterns, prognostic value, and genetic mutations of 7 hub FRGs in ccRCC.Conclusions: Collectively, we successfully constructed a novel ferroptosis-related risk signature that was significantly associated with the prognosis of ccRCC.
Study on Multi Stage Forging Process with Combination of Different Strain Rate and Temperature Regime in IMI 685 Forging
Authors: A Jayanthi , M Anil Kumar , Bhat Raghavendra R , B Ravisankar 
Published: February 2021

Abstract
IMI 685, a near alpha Ti alloy has been used as high pressure compressor discs for aero engine application due to its high strength to weight ratio and high creep resistance. Compressor disc is a very critical component in an aero engine and manufacturing process plays an important role in obtaining the required mechanical properties. Generally due to a highly complex and critical product, its manufacturing process is not available in open literatures. Currently, isothermal forging is used and which is time consuming and not cost effective. Hence, in this paper non-isothermal approach of manufacturing compressor disc using a combination of hydraulic press and pneumatic hammer have been explored as an alternate. Simulation studies have been carried out using DEFORM 3D FEM software for the prediction of temperature distribution, strain and strain rate experienced during forging. Forging has been carried out in two stages, hydraulic press used for upsetting and hammer used for die forging in partial α-b / b temperature range and beta solutionised followed by aging. Extensive mechanical testing such as room temperature tensile test, hot tensile test, notch tensile test, creep, post creep tensile test and micro structural characterization has been performed. From the mechanical and metallurgical analysis it was found that processing in partial α-b / b temperature range resulted in higher creep strain rate with acicular / needle alpha platelets with a basket weave structure. This experiment has proven with a proper selection of thermo mechanical process parameter, required creep properties can be met with the non-isothermal processing route.
Key Potentials of mHealth for Community-Based Early Detection of Cardiovascular Disease During the COVID-19 Pandemic
Authors: Siregar Kemal Nazarudin , Kurniawan Rico , BaharuddinNur Ryza Jazid , Nuridzin Dion Zein , Handayani Yolanda , Retnowati Retnowati , Rohjayanti Rohjayanti , Halim Lindawati 
Published: February 2021

Abstract
Background

The epidemiological transition from infectious to non-communicable disease (NCD) is characterized by an increasing incidence of cardiovascular disease (CVD). The Coronavirus Disease 2019 (COVID-19) pandemic has led to a significant decline in NCD screening and treatment activities in health centers. This study aims to revive and expand the coverage of NCD control programs, from the elderly to productive age groups, through the use of mHealth for the early detection of CVD, which is also provides health promotion media that is easily accessible.
Methods

This research is an operational study to develop a community-based early detection mechanism for CVD using mHealth during the COVID-19 pandemic in the Babakan Madang sub-district, Bogor district.
Results

The use of the mHealth application supported by community participation is proven to be able to reach the productive age population significantly (87.1%) in the Babakan Madang sub-district. The mHealth application simplifies CVD risk predictions so that it can be used by the public during the COVID-19 pandemic.
Conclusion

This application is also very well accepted by the community and is able to provide personalized health promotions.
Clinical features of extrahepatic recurrence after curative hepatectomy for hepatocellular carcinoma: simple parameters predicting extrahepatic recurrence
Authors: Yoon Jae Hyun , Lee Won Jae , Kim Sun Min , Kim Kwang Tack , Kim Hee Joon , Ko Yang Seok , Kook Hyun Yi , Jun Chung Hwan , Cho Sung Bum , Choi Sung Kyu 
Published: February 2021

Abstract
Background

Extrahepatic recurrence (EHR) after curative hepatectomy for hepatocellular carcinoma (HCC) is associated with a poor prognosis. We investigated the features of EHR and identified its predictive factors.
Methods

This retrospective study included 398 treatment-naive patients who underwent curative hepatectomy for HCC at two tertiary hospitals. Multivariate analysis via Cox-regression was performed to identify the variables associated with EHR.
Results

EHR was diagnosed in 94 patients (23.6%) over a median follow-up period of 5.92 years, most commonly in the lungs (42.6%). The 5-/10-year cumulative rates of HCC recurrence and EHR were 63.0%/75.6% and 18.1%/35.0%, respectively.. The median time to EHR was 2.06 years. Intrahepatic HCC recurrence was not observed in 38.3% of patients on EHR diagnosis. On multivariate analysis, bile duct invasion, tumor necrosis, sum of tumor size > 7 cm, macrovascular invasion, first recurrence free survival < 1 year, and serum alpha fetoprotein > 400 IU/mL during recurrence were predictive of EHR. Four risk levels and their respective EHR were defined: very low risk, 2-/5-year, 0.7%/14.2%; low risk, 2-/5-year, 6.4%/31.0%; intermediate risk, 2-/5-year, 21.9%/73.1%; and high risk, 2-/3-year, 70.8%/100.0%.
Conclusion

Our predictive model clarifies the clinical course of EHR and could improve the follow-up strategy to improve outcomes.
Spatiotemporal Imaging and Pharmacokinetic of Fluorescent Compounds in Zebrafish Eleuthero-Embryos After Different Routes of Administration
Authors: Guarin Marlly , Faelens Ruben , Giusti Arianna , Croze Noémie De , Léonard Marc , Cabooter Deirdre , Annaert Pieter , Witte Peter de , Ny Annelii 
Published: February 2021

Abstract
Zebrafish (Danio rerio) is increasingly used to assess the pharmacological activity and toxicity of compounds. The spatiotemporal distribution of seven fluorescent alkyne compounds was examined during 48 h after immersion (10 µM) or microinjection (2 mg/kg) in the pericardial cavity (PC), intraperitoneally (IP) and yolk sac (IY) of 3 dpf zebrafish eleuthero-embryos. By modelling the fluorescence of whole-body contours present in fluorescence images, the main pharmacokinetic (PK) parameter values of the compounds were determined. It was demonstrated that especially in case of short incubations (1-3h) immersion can result in limited intrabody exposure to compounds. In this case, PC and IP microinjections represent excellent alternatives. Significantly, IY microinjections did not result in a suitable intrabody distribution of the compounds. Performing a QSPkR (quantitative structure-pharmacokinetic relationship) analysis, LogD was identified as the only molecular descriptor that explains the final uptake of the selected compounds. It was also shown that combined administration of compounds (immersion and microinjection) provides a more stable intrabody exposure, at least in case of a prolonged immersion and compounds with LogD value > 1. These results will help reduce the risk of false negative results and can offer an invaluable input for future translational research and safety assessment applications.
The Role of F18-FLT PET / CT in Assessing Early Response to TARE and TACE in Patients with Primary and Metastatic Liver Tumors
Authors: NAK DEMET , Kucuk Nuriye Ozlem , Celebioglu Emre Can , BILGIC Mehmet Sadik , HAYME Serhat , KIR Metin Kemal 
Published: February 2021

Abstract
ObjectiveThis study aims to identify F18-FLT PET / CT's role in the assessment of early response to intraarterial chemoembolization and radioembolization treatments in patients with unresectable primary and metastatic liver tumors.MethodsThis single-center study included 63 patients who underwent F18-FLT PET / CT for response evaluation after TACE and TARE treatments. After excluding 20 patients due to various reasons, 43 patients were analyzed. The compatibility of change in semi-quantitative values obtained from the F18-FLT PET / CT images with the treatment responses detected in F18-FDG PET / CT, CT, and MR images was evaluated.ResultsThere was no correlation between early metabolic, morphological response, F18-FLT visual change, ΔSUVmax, ΔSUVmean, and ΔSUVpeak values. There was no significant correlation between F18-FLT visual change, ΔSUVmax, ΔSUVmean, ΔSUVpeak, and OS, PFS for the target lobe PFS for the whole-body. The survival distributions for the patients with and without >30% change in ΔSUVmax and ΔSUVpeak values were statistically significantly different (p < .009 and p < .024, respectively).ConclusionIt has not been demonstrated that F18-FLT PET / CT imaging is one of the methodologies that should be applied primarily in monitoring early response to embolization treatment in patients with unresectable primary and metastatic liver tumors.
Comparison of biplane manual, automated volumetric and MRI methods for assessment of left ventricle volumes and function in a series of three left ventricle non-compaction patients
Authors: Pigon Katarzyna Justyna , Radzik Edyta , Krzemień-Wolska Krystyna , Przywara-Chowaniec Brygida , Nowalany-Kozielska Ewa , Tomasik Andrzej 
Published: February 2021

Abstract
Background

Non-compaction of the ventricular myocardium (LVNC) is a rare genetically determined heart defect. The disease is most frequently detected during accidental echocardiography. There is no golden rule examination for LVNC diagnosis. In our study, we aimed to compare the measurements of the left ventricle volumes and function obtained with the two-dimensional, and three-dimensional echo based on the results from MR imaging in a series of three left ventricle non-compaction cardiomyopathy patients.
Case presentation

Patients’ age was 43–53 years. Two patients presented with congestive heart failure, third patient presented with ventricular arrhythmia. The proportion of thickness of non-compacted to compacted layers ranged from 2.3 to 3.6. Three-dimensional echo has revealed substantially higher end-diastolic and end-systolic volumes in comparison to two-dimensional echo, yet not as high as assessed in magnetic resonance imaging. Speckle tracking revealed reduced global longitudinal, radial, and circumferential strain, even in the patients with normal left ventricle function.
Conclusion

Real-time three-dimensional echocardiography with automated left ventricle analysis offers more information on the assessment of left ventricle in patients with left ventricle non-compaction in comparison to two-dimensional echocardiography. Speckle tracking analysis may add prognostic information in this specific group of patients.
Synthesis of self-assembled nacre-like materials with 3D periodic layers from nanochitin via hydrogelation and mineralization
Authors: Xu Junhua , Liu Liang , Yu Juan , Zou Yujun , Pei Wenhui , Zhang Lili , Ye Wenbo , Bai Long , Wang Zhiguo , Fan Yimin , Yong Qiang , Rojas Orlando 
Published: February 2021

Abstract
Here introduced a route for the synthesis of 3D structures that display a mechanical strength that competes with that of the toughest materials found in nature. Following the “brick-and-mortar” biomineralization typical of nacre, self-stratified, periodic materials are obtained by one-step ion diffusion gradient and hydrogelation of nanochitin with simultaneous mineral coprecipitation. Specifically, under appropriate electrolyte conditions, hydroxyapatite (HA) microspheres grow in an organic network formed from partially deacetylated chitin nanofibers (NCh), resulting in periodic stacking of mineralized (HA) and non-mineralized (NCh) layers. By directional diffusion, customizable 3D shapes are self-assembled and demonstrated to function as optical waveguides with selective light transmission at interfaces. Upon hot pressing, the resulting solid structures exhibit a superb mechanical performance while being biocompatible (tested with chondrogenic ATDC5 cells as a model for physiological mineralization). Overall, a shape-controlled, one-pot biomineralization method was proposed that achieves hierarchical, periodic and strong “nacre-like” structures suitable as biomedical material.
The effect of oxidant-antioxidant balance on the one-year prognosis of patients with acute ischemic stroke: a case-control study
Authors: Shaafi Sheida , Hadisi Fina , Mahmoudinezhad Mahsa , Razmi Hamidreza , Nejadghaderi Seyed Aria , Khalili Mohammad 
Published: February 2021

Abstract
Background

Stroke is a major cause of mortality and morbidity. Also, free radicals and oxidative stress are deleterious factor in the stroke progression. We aimed to evaluate the association between oxidative stress markers and odds of having risk factor for stroke or developing stroke.
Methods

The present case control study conducted on 556 participants in Imam-Reza hospital, Tabriz, Iran. Subjects were divided into three group, including individuals with acute ischemic stroke, at risk of stroke, and healthy controls. All enrolled participants except for controls underwent neurological examinations and brain magnetic resonance imaging (MRI). Stroke-related disability and stroke severity were evaluated by modified Rankin Scale (mRS) and National Institutes of Health Stroke Scale (NIHSS), respectively. Serum malondialdehyde (MDA) level and total antioxidant capacity (TAC) were measured within 48 hours of stroke. One-way ANOVA and Chi-square tests for comparing characteristics between groups, multivariable logistic regression for odds of stroke based on MDA and TAC quartiles, and Spearman’s correlation were used.
Results

Serum MDA was significantly higher in stroke group than controls in addition to systolic and diastolic blood pressure, cholesterol, and triglyceride. Higher levels of MDA increased odds of stroke development (P < 0.001), however TAC and MDA were not associated with having risk factors for stroke (P = 1.00 and 0.27, respectively). Also, TAC level was negatively associated with baseline (ρ=-0.28; P = 0.04) and follow-up (ρ=-0.31; P = 0.03) NIHSS scores. Moreover, MDA was correlated with mRS score at follow-up (ρ=-0.26; P = 0.04).
Conclusions

The balance between antioxidants and oxidants markers might reveal a new approach in this context. Despite recent efforts to identify the source of oxidative stress as well as cessation of the production of oxygen radicals in stroke, further studies are warranted.
WITHDRAWN: Utilization of Sustainable Energy in a Novel Architecture
Authors: Haseeb Qubad , al-bayaty Hussein , Abdulkarim Ali 
Published: February 2021

Abstract
This research deals with a novel sustainable architecture through future prospects for the development of this concept. ANSYS software simulation has been used for a real mosque design located in the north of Iraq as a case study. Hence the selection of mosque buildings in Sulaimaniyah city in Iraq as an applied model for the use of clean energy technologies by selecting the internal space of the building and conducting tests using solar energy and studying its impact on providing the energy needed by the building and reducing the waste in electrical energy consumption that depends on fossil resources.
This study assumes that the foundations of a novel constructed mosque can be covered with a grid of pipes distributed equally throughout the design. These pipes can be filled with hot water in winter instead of using heaters which cost a lot and consume a large amount of electricity. In addition, it shows the feasibility of using green energy as a heating unit, especially when it can cover the capital cost of installation in just one year because of the high electricity bills in Iraq and the ability to maintain solar energy.
Circ-BANP Contributes to Cell Carcinogenesis and Aerobic Glycolysis by Regulating MAPK1 Through miR-874-3p in Colorectal Cancer
Authors: Zhang Yunxin , Shen Kexin , Zha Hanyi , Zhang Wentao , Zhang Haishan 
Published: February 2021

Abstract
BackgroundCircular RNA-BTG3 associated nuclear protein (circ-BANP) was identifified to involve in cell proliferation of colorectal cancer (CRC). The aerobic glycolysis is a key metabolism mediating cancer progression. However, the role of circ-BANP on aerobic glycolysis in CRC remains unknown. MethodsThe expression of circ-BANP, microRNA (miR)-874-3p, and mitogen-activated protein kinase 1 (MAPK1) mNRA was detected using quantitative real-time polymerase chain reaction. Cell viability and invasion were measured by cell counting kit-8 assay or transwell assay. Glucose consumption and lactate production were assessed by a glucose and lactate assay kit. XF Extracellular Flux Analyzer was used to determine extracellular acidifification rate (ECAR). Western blot was used to analyze the levels of hexokinase-2 (HK2), pyruvate kinase M2 (PKM2), MAPK1, proliferating cell nuclear antigen (PCNA), Cyclin D1, N-cadherin, E-cadherin, hypoxia inducible factor-1α (HIF-1α), glucose transport protein 1(GLUT1), and c-Myc. The interaction between miR-874-3p and circ-BANP or MAPK1 was confifirmed by dual luciferase reporter assay. In vivo experiments were conducted through the murine xenograft model. ResultsCirc-BANP was up-regulated in CRC tissues and cell lines. Circ-BANP knockdown suppressed CRC cell proliferation, invasion and aerobic glycolysis in vitro as well as inhibited tumor growth in vivo. Circ-BANP was a sponge of miR-874-3p and performed anti-tumor effffects by binding to miR-874-3p in CRC cells. Subsequently, we confifirmed MAPK1 was a target of miR-874-3p and circ-BANP indirectly regulated MAPK1 expression by sponging miR-874-3p. After that, we found MAPK1 overexpression partially reversed circ-BANP deletion-mediated inhibition on cell carcinogenesis and aerobic glycolysis in CRC. ConclusionCirc-BANP accelerated cell carcinogenesis and aerobic glycolysis by regulating MAPK1 through miR- 874-3p in CRC, suggesting a promising therapeutic strategy for CRC treatment.
Folate Deficiency in Early Pregnancy Increases the Risk of Low Birth Weight: Survey of Neonates in Eastern China
Authors: Chen Yisheng , Ying Chunmei 
Published: February 2021

Abstract
Objective: Low birth weight (LBW) is a major cause of fetal mortality and morbidity. This study aims to assess the relationship between maternal serum folate levels and LBW in early pregnancy in eastern China. Methods: We conducted a retrospective study including 124 newborns with LBW (born≥1500g to <2500 g) and 393 normal birth weight neonates (NBW). The maternal methylenetetrahydrofolate reductase (MTHFR) C677T polymorphism genotyping was performed by the gene chip hybrid method. The concentrations of serum homocysteine (hcy), folate, vitamin B12 and vitamin D were measured before 18 weeks of pregnancy. Multivariable logistic regression model was used to identify the predictors of LBW. Results: After controlling the covariates, maternal underweight before pregnancy (AOR = 5.15: 95% CI 2.40, 11.05), folate deficiency in early pregnancy (AOR = 2.06: 95% CI 1.03, 4.09), preeclampsia during pregnancy (AOR = 6.02: 95% CI 2.83, 12.81), and premature delivery (AOR =23.81: 95% CI 12.90, 43.95) were risk factors for LBW. However, multipara (AOR = 0.53:95% CI 0.31, 0.91) was a protective factor for LBW. Conclusions: This study revealed different factors associated with LBW in eastern China. Sufficient folate before 18 weeks of pregnancy is a protective factor for LBW. Serum folate > 32.5 nmol/L can reduce the risk of LBW. Therefore, maternal screening of folate statue in the first trimester of pregnancy could be helpful.
Impact of GBA2 on Neuronopathic Gaucher’s Disease and α-Synuclein Accumulation in Medaka (Oryzias Latipes)
Authors: Nakanishi Etsuro , Uemura Norihito , Akiyama Hisako , Kinoshita Masato , Sawamura Masanori , Taruno Yosuke , Yamakado Hodaka , Matsuzawa Shu-ichi , Takeda Shunichi , Hirabayashi Yoshio , Takahashi Ryosuke 
Published: February 2021

Abstract
Homozygous mutations in the lysosomal glucocerebrosidase gene, GBA1, cause Gaucher’s disease (GD), while heterozygous mutations in GBA1 are a strong risk factor for Parkinson’s disease (PD), whose pathological hallmark is intraneuronal α-synuclein (asyn) aggregates. We previously reported that GBA1 knockout (KO) medaka exhibited glucosylceramide accumulation and neuronopathic GD phenotypes, including short lifespan, non-selective neuron loss, microglial activation, and swimming abnormality, with asyn accumulation in the brains. A recent study reported that deletion of GBA2, non-lysosomal glucocerebrosidase, in a non-neuronopathic GD mouse model rescued its phenotypes. In the present study, we generated GBA2 KO medaka and examined the effect of GBA2 deletion on the phenotypes of GBA1 KO medaka. The GBA2 deletion in GBA1 KO medaka resulted in the exacerbation of glucosylceramide accumulation and no improvement in neuronopathic GD pathological changes, asyn accumulation, or swimming abnormalities. Meanwhile, though GBA2 KO medaka did not show any apparent phenotypes, biochemical analysis revealed asyn accumulation in the brains. GBA2 KO medaka showed a trend towards an increase in sphingolipids in the brains, which is one of the possible causes of asyn accumulation. In conclusion, this study demonstrated that the deletion of GBA2 does not rescue the pathological changes or behavioral abnormalities of GBA1 KO medaka, and GBA2 represents a novel factor affecting asyn accumulation in the brains.