bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cytomegalovirus-specific T-cell reconstitution following letermovir prophylaxis after hematopoietic cell transplantation
Authors: Zamora Danniel , Duke Elizabeth R. , Xie Hu , Edmison Bradley C. , Akoto Brenda , Kiener Richard , Stevens-Ayers Terry , Wagner Ralf , Mielcarek Marco , Leisenring Wendy M. , Jerome Keith R. , Schiffer Joshua T. , Finak Greg , De Rosa Stephen C. , Boeckh Michael 
Published: March 2021

AbstractDecreased cytomegalovirus (CMV)-specific immunity after hematopoietic cell transplantation (HCT) is associated with late CMV reactivation and increased mortality. Whether letermovir prophylaxis-associated reduction in viral exposure influences CMV-specific immune reconstitution is unknown. In a prospective cohort of allogeneic HCT recipients who received letermovir, we compared polyfunctional CMV-specific T-cell responses to those of controls who received PCR-guided preemptive therapy before the introduction of letermovir. Thirteen-color flow cytometry was used to assess T-cell responses at 3 months after HCT following stimulation with CMV immediate early-1 (IE-1) antigen and phosphoprotein 65 (pp65) antigens. Polyfunctionality was characterized by combinatorial polyfunctionality analysis of antigen-specific T-cell subsets. Use of letermovir and reduction of viral exposure were assessed for their association with CMV-specific T-cell immunity. Polyfunctional T-cell responses to IE-1 and pp65 were decreased in letermovir recipients and remained diminished after adjustment for donor CMV serostatus, absolute lymphocyte count, and steroid use. Among letermovir recipients, greater peak CMV DNAemia and increased viral shedding were associated with stronger CD8+ responses to pp65, whereas the CMV shedding rate was associated with greater CD4+ responses to IE-1. In summary, our study provided initial evidence that letermovir may delay CMV-specific cellular reconstitution, possibly related to decreased CMV antigen exposure. Evaluating T-cell polyfunctionality may identify patients at risk for late CMV infection after HCT.
Reduced calf muscle pump function is a risk factor for venous thromboembolism: a population-based cohort study
Authors: Houghton Damon E. , Ashrani Aneel , Liedl David , Mehta Ramila A. , Hodge David O. , Rooke Thom , Wennberg Paul , Wysokinski Waldemar , McBane Robert 
Published: March 2021

Abstract
The calf muscle pump is a major determinate of venous return in the legs but has not been studied as a risk factor for venous thromboembolism (VTE). A population-based cohort study of Olmsted County, Minnesota residents was performed using calf pump function (CPF) measurements from venous plethysmography studies from 1998 to 2015. Patients with a history of VTE were excluded. Nursing validated VTE outcomes from the Rochester Epidemiology Project were identified after the index study date, and patients with reduced CPF (rCPF) were compared with patients with normal CPF. A total of 1532 patients with recorded CPF (28% air and 72% strain gauge plethysmography) were included; 591 (38.5%) had normal CPF, 353 (23.0%) had unilateral rCPF, and 588 (38.3%) had bilateral rCPF. Any VTE occurred in 87 patients (5.7%) after a median follow-up of 11.7 years (range, 0-22.0 years). Comparing patients with bilateral reduced to bilateral normal CPF, the unadjusted hazard ratio (HR) for incident VTE was 2.0 (95% confidence interval [CI], 1.2-3.4) and after adjusting for age, BMI, and Charlson Comorbidity Index, the HR was 1.68 (95% CI, 0.98-2.89). The adjusted HR for ipsilateral deep vein thrombosis was evaluated in 3064 legs comparing legs with reduced to normal CPF and was 1.71 (95% CI, 1.03-2.84). Mortality was significantly higher in both the bilateral (P &lt; .001) and unilateral (P &lt; .001) rCPF groups compared with normal CPF. Our results demonstrate that CPF is a risk factor for VTE in an otherwise low-risk ambulatory population and might be a useful component in risk stratification models.
Structural and thermal evolution of the eastern Aar Massif: insights from structural field work and Raman thermometry
Authors: Nibourel Lukas , Berger Alfons , Egli Daniel , Heuberger Stefan , Herwegh Marco 
Published: March 2021

AbstractThe thermo-kinematic evolution of the eastern Aar Massif, Swiss Alps, was investigated using peak temperature data estimated from Raman spectroscopy of carbonaceous material and detailed field analyses. New and compiled temperature-time constraints along the deformed and exhumed basement-cover contact allow us to (i) establish the timing of metamorphism and deformation, (ii) track long-term horizontal and vertical orogenic movements and (iii) assess the influence of temperature and structural inheritance on the kinematic evolution. We present a new shear zone map, structural cross sections and a step-wise retrodeformation. From$$\text{ca.\;26\,Ma}$$ca.\;26\,Maonwards, basement-involved deformation started with the formation of relatively discrete NNW-directed thrusts. Peak metamorphic isograds are weakly deformed by these thrusts, suggesting that they initiated before or during the metamorphic peak under ongoing burial in the footwall to the basal Helvetic roof thrust. Subsequent peak- to post-metamorphic deformation was dominated by steep, mostly NNW-vergent reverse faults ($$\text{ca.}$$ca. 22–14 Ma). Field investigations demonstrate that these shear zones were steeper than$$50^{\circ}$$50∘already at inception. This produced the massif-internal structural relief and was associated with large vertical displacements (7 km shortening vs. up to 11 km exhumation). From 14 Ma onwards, the eastern Aar massif exhumed “en bloc” (i.e., without significant differential massif-internal exhumation) in the hanging wall of frontal thrusts, which is consistent with the transition to strike-slip dominated deformation observed within the massif. Our results indicate 13 km shortening and 9 km exhumation between 14 Ma and present. Inherited normal faults were not significantly reactivated. Instead, new thrusts/reverse faults developed in the basement below syn-rift basins, and can be traced into overturned fold limbs in the overlying sediment, producing tight synclines and broad anticlines along the basement-cover contact. The sediments were not detached from their crystalline substratum and formed disharmonic folds. Our results highlight decreasing rheological contrasts between (i) relatively strong basement and (ii) relatively weak cover units and inherited faults at higher temperature conditions. Both the timing of basement-involved deformation and the structural style (shear zone dip) appear to be controlled by evolving temperature conditions.
Support, needs and expectations of family caregivers regarding general practitioners – results from an online survey
Authors: Wangler Julian , Jansky Michael 
Published: March 2021

Abstract
Background
Family caregivers are often the first line of support for people requiring care; although they may personally stand to benefit, these activities substantially increase the risk of physical and emotional stress. General practitioners (GPs) may provide important support and stabilisation, but need to adjust to the needs and expectations of this group in order to do so. The aim of the study was to compare the needs of family caregivers from GPs to the support they actually experience. Additional aims included determining the main factors affecting satisfaction amongst family caregivers with support from GPs. The results were used to develop possible approaches towards optimisation within the purview of general medical practice.

Methods
Between January and July 2020, 612 people supporting or caring for a family member responded to an online survey posted in seventeen internet forums focused on family caregivers. In addition to the descriptive analysis, a t-test with independent samples was used to identify significant differences between two groups. We also used binary logistic regression analysis to identify indications of potentially influential factors regarding the experienced support from GPs.

Results
Around three out of every four respondents (72%) consulted GPs in care matters. The respondents gave positive responses on their GP’s knowledge of the care situation (71%), approachability in various issues connecting with care and service towards the caregiver (82%). GPs’ efforts in meeting the needs and requirements of the care recipient were also rated positively (82%). Weaknesses in support from GPs mainly involved the lack of information on advice and assistance services (55%) as well as frequently not identifying or involving caregivers as such soon enough (42%). Results from regression analysis show that the last two aspects play a major role in subjective satisfaction amongst family caregivers with support from GPs.

Conclusions
We recommend that GPs undergo further training to reinforce awareness that the care triad of needs, requirements and stresses amongst family caregivers also plays a vital role in care outcomes. With this in mind, general practice staff should adopt a pre-emptive strategy towards approaching family members about potential issues and informing them about existing assistance and support services.
Independent and sensitive gait parameters for objective evaluation in knee and hip osteoarthritis using wearable sensors
Authors: Boekesteijn Ramon J. , Smolders José M. H. , Busch Vincent J. J. F. , Geurts Alexander C. H. , Smulders Katrijn 
Published: March 2021

Abstract
Background
Although it is well-established that osteoarthritis (OA) impairs daily-life gait, objective gait assessments are not part of routine clinical evaluation. Wearable inertial sensors provide an easily accessible and fast way to routinely evaluate gait quality in clinical settings. However, during these assessments, more complex and meaningful aspects of daily-life gait, including turning, dual-task performance, and upper body motion, are often overlooked. The aim of this study was therefore to investigate turning, dual-task performance, and upper body motion in individuals with knee or hip OA in addition to more commonly assessed spatiotemporal gait parameters using wearable sensors.

Methods
Gait was compared between individuals with unilateral knee (n = 25) or hip OA (n = 26) scheduled for joint replacement, and healthy controls (n = 27). For 2 min, participants walked back and forth along a 6-m trajectory making 180° turns, with and without a secondary cognitive task. Gait parameters were collected using 4 inertial measurement units on the feet and trunk. To test if dual-task gait, turning, and upper body motion had added value above spatiotemporal parameters, a factor analysis was conducted. Effect sizes were computed as standardized mean difference between OA groups and healthy controls to identify parameters from these gait domains that were sensitive to knee or hip OA.

Results
Four independent domains of gait were obtained: speed-spatial, speed-temporal, dual-task cost, and upper body motion. Turning parameters constituted a gait domain together with cadence. From the domains that were obtained, stride length (speed-spatial) and cadence (speed-temporal) had the strongest effect sizes for both knee and hip OA. Upper body motion (lumbar sagittal range of motion), showed a strong effect size when comparing hip OA with healthy controls. Parameters reflecting dual-task cost were not sensitive to knee or hip OA.

Conclusions
Besides more commonly reported spatiotemporal parameters, only upper body motion provided non-redundant and sensitive parameters representing gait adaptations in individuals with hip OA. Turning parameters were sensitive to knee and hip OA, but were not independent from speed-related gait parameters. Dual-task parameters had limited additional value for evaluating gait in knee and hip OA, although dual-task cost constituted a separate gait domain. Future steps should include testing responsiveness of these gait domains to interventions aiming to improve mobility.
Reproducibility and relative validity of a semi-quantitative food frequency questionnaire for the Chinese lactating mothers
Authors: Ding Ye , Li Fang , Hu Ping , Ye Mei , Xu Fangping , Jiang Wei , Yang Yue , Fu Youjuan , Zhu Yunhua , Lu Xiaolong , Liu Ying , Xie Zhencheng , Wang Zhixu 
Published: March 2021

Abstract
Background
The dietary nutritional status of the lactating mothers is related to maternal health and has a significant impact on the growth and development of infants through the secretion of breast milk. The food frequency questionnaire (FFQ) is the most cost-effective dietary assessment method that can help obtain information on the usual dietary pattern of participants. Until now, the FFQs have been used for different populations in China, but there are few FFQs available for the lactating mothers. We aimed to develop a semi-quantitative, 156-item FFQ for the Chinese lactating mothers, and evaluate its reproducibility and relative validity.

Methods
A total of 112 lactating mothers completed two FFQs and one 3-d dietary record (3DR). The first FFQ (FFQ1) was conducted during postpartum at 60–65 days and the second FFQ (FFQ2) during subsequent follow-up at 5 weeks. The 3DR was completed with portion sizes assessed using photographs taken by the respondent before and after eating (instant photography) 1 week after FFQ1.

Results
For reproducibility, the Spearman’s correlation coefficients for food ranged from 0.34 to 0.68, and for nutrients from 0.25 to 0.61. Meanwhile, the intra-class correlation coefficients for food ranged from 0.48 to 0.87, and for nutrients from 0.27 to 0.70. For relative validity, the Spearman’s correlation coefficients for food ranged from 0.32 to 0.56, and for nutrients from 0.23 to 0.72. The energy-adjusted coefficients for food ranged from 0.26 to 0.55, and for nutrients from 0.22 to 0.47. Moreover, the de-attenuation coefficients for food ranged from 0.34 to 0.67, and for nutrients from 0.28 to 0.77. The Bland-Altman plots also showed reasonably acceptable agreement between the two methods.

Conclusions
This FFQ is a reasonably reproducible and a relative valid tool for assessing dietary intake of the Chinese lactating mothers.
mRNA in cancer immunotherapy: beyond a source of antigen
Authors: Van Hoecke Lien , Verbeke Rein , Dewitte Heleen , Lentacker Ine , Vermaelen Karim , Breckpot Karine , Van Lint Sandra 
Published: March 2021

AbstractmRNA therapeutics have become the focus of molecular medicine research. Various mRNA applications have reached major milestones at high speed in the immuno-oncology field. This can be attributed to the knowledge that mRNA is one of nature’s core building blocks carrying important information and can be considered as a powerful vector for delivery of therapeutic proteins to the patient.For a long time, the major focus in the use of in vitro transcribed mRNA was on development of cancer vaccines, using mRNA encoding tumor antigens to modify dendritic cells ex vivo. However, the versatility of mRNA and its many advantages have paved the path beyond this application. In addition, due to smart design of both the structural properties of the mRNA molecule as well as pharmaceutical formulations that improve its in vivo stability and selective targeting, the therapeutic potential of mRNA can be considered as endless.As a consequence, many novel immunotherapeutic strategies focus on the use of mRNA beyond its use as the source of tumor antigens. This review aims to summarize the state-of-the-art on these applications and to provide a rationale for their clinical application.
Comparison of the inner side and two-sided approaches for iliac crest bone graft harvesting for pediatric pelvic osteotomy
Authors: Chen Xin , Chen Kai , Su Yuxi 
Published: March 2021

Abstract
Background
The iliac crest is one of the most used bone graft sources. In this study, we aimed to identify the effects of inner side and two-sided approaches for iliac crest bone harvesting on post-surgery ilium growth in children.

Materials and methods
We retrospectively analyzed 47 patients who underwent pelvic osteotomy and iliac crest bone graft (ICBG) procedures from January 2015 to September 2018. The patients were divided into an inner table ilium exposure group (group A) and the inner-outer table ilium exposure group (group B) and were followed up with radiography in postoperative months 1, 3, 6, and 12, and the growth areas were measured using PACS software. Complications such as damage to the arteries or nerves, ureteral injury, gastrointestinal hernia, ileus, abnormal cosmetic appearance, sensory disturbances, and functional limitations were recorded based on clinical records.

Results
There were 22 patients aged 5.3±1.5 years in group A and 25 patients aged 5.9±1.8 years in group B. There were no significant differences in demographics between the two groups, or in growth in the first month. However, bone graft growth at months 3, 6, and 12 was significantly better in group A than in group B. There was no significant difference in complications between the two groups.

Conclusion
Exposure of only the inner table of the ilium resulted in faster recovery of the bone defect than two-sided exposure in pelvic osteotomy. Therefore, we suggest protecting the outer side of the ilium during surgery.

Level of evidence
Level III
Severe primary hypothyroidism in an apparently asymptomatic 19-year-old woman: a case report
Authors: Dannan Rania , Hajji Sulaiman , Aljenaee Khaled 
Published: March 2021

AbstractBackgroundHypothyroidism is diagnosed on the basis of laboratory tests because of the lack of specificity of the typical clinical manifestations. There is conflicting evidence on screening for hypothyroidism.Case presentationWe report a case of an apparently healthy 19-year-old Kuwaiti woman referred to our clinic with an incidental finding of extremely high thyroid-stimulating hormone (TSH), tested at the patient’s insistence as she had a strong family history of hypothyroidism. Despite no stated complaints, the patient presented typical symptoms and signs of hypothyroidism on evaluation. Thyroid function testing was repeated by using different assays, with similar results; ultrasound imaging of the thyroid showed a typical picture of thyroiditis. Treatment with levothyroxine alleviated symptoms and the patient later became biochemically euthyroid on treatment.ConclusionThere is controversy regarding screening asymptomatic individuals for hypothyroidism; therefore, it is important to maintain a high index of suspicion when presented with mild signs and symptoms of hypothyroidism especially with certain ethnic groups, as they may be free of the classical symptoms of disease.
New Hermite–Hadamard-type inequalities for "Equation missing"-convex fuzzy-interval-valued functions
Authors: Khan Muhammad Bilal , Noor Muhammad Aslam , Noor Khalida Inayat , Chu Yu-Ming 
Published: March 2021

AbstractIn this paper, we introduce the non-convex interval-valued functions for fuzzy-interval-valued functions, which are called "Equation missing"-convex fuzzy-interval-valued functions, by means of fuzzy order relation. This fuzzy order relation is defined level-wise through Kulisch–Miranker order relation given on the interval space. By using the "Equation missing"-convexity concept, we present fuzzy-interval Hermite–Hadamard inequalities for fuzzy-interval-valued functions. Several exceptional cases are debated, which can be viewed as useful applications. Interesting examples that verify the applicability of the theory developed in this study are presented. The results of this paper can be considered as extensions of previously established results.
Launching graphene surface plasmon waves with vanishingly small periodic grating structures
Authors: Nicholls David P. , Oh Sang-Hyun 
Published: March 2021

Graphene is now a crucial component of many device designs in electronics and optics. Just like the noble metals, this single layer of carbon atoms in a honeycomb lattice can support surface plasmons, which are central to several sensing technologies in the mid-infrared regime. As with classical metal plasmons, periodic corrugations in the graphene sheet itself can be used to launch these surface waves; however, as graphene plasmons are tightly confined, the role of unwanted surface roughness, even at a nanometer scale, cannot be ignored. In this work, we revisit our previous numerical experiments on metal plasmons launched by vanishingly small grating structures, with the addition of graphene to the structure. These simulations are conducted with a recently devised, rapid, and robust high-order spectral scheme of the authors, and with it we carefully demonstrate how the plasmonic response of a perfectly flat sheet of graphene can be significantly altered with even a tiny corrugation (on the order of merely 5 nm). With these results, we demonstrate the primary importance of fabrication techniques that produce interfaces whose deviations from flat are on the order of angstroms.
Ultrafast 3.5  µm fiber laser
Authors: Bawden Nathaniel , Henderson-Sapir Ori , Jackson Stuart D. , Ottaway David J. 
Published: March 2021

We report, to the best of our knowledge, the first mode-locked fiber laser to operate in the femtosecond regime well beyond 3 µm. The laser uses dual-wavelength pumping and nonlinear polarization rotation to produce 3.5 µm wavelength pulses with minimum duration of 580 fs at a repetition rate of 68 MHz. The pulse energy is 3.2 nJ, corresponding to a peak power of 5.5 kW.
Potential linkage between Toxoplasma gondii infection and physical education scores of college students
Authors: Cai Jun , Sheng Zhijin , Jin Yu , Du Yinan , Yan Xinlei , Yao Yong 
Published: March 2021

Objective
Toxoplasma gondii is a worldwide protozoan parasite that could infect virtually all warm-blooded animals, including humans. Our study aimed to investigate the prevalence of T. gondii infection in college students at Anhui province, China and to assess risk factors for T. gondii infection in college students. Moreover, growing studies demonstrated the association between T. gondii infection and host behavioral changes. We also studied the linkage between T. gondii and physical education (PE) scores of college students.

Methods
A total of 2704 serum samples of medical school students attending physical education lessons were collected from September 2017 to September 2019 and evaluated for T. gondii IgG antibodies using an enzyme-linked immunosorbent assay (ELISA). Questionnaires and statistical analysis were used to determine the risk factors for T. gondii infection. We also analysed PE scores of T. gondii -infected students and T. gondii-uninfected students.

Results
The overall seroprevalence of T. gondii was 11.5%. The main risk factors related to T. gondii infection in college students were cat in the household and gardening or agriculture activity. Furthermore, in the basketball group and the soccer group, scores of T. gondii seropositive students were significantly higher than those of seronegative students, while in other sports there was no difference between scores of T. gondii-infected students and T. gondii uninfected students.

Conclusion
This is the first report of T. gondii seroprevalence in college students in Anhui province, China.