bluuebunny/crossref_metadata_2025_split huggingface dataset:
MicroRNA Expression in Circulating Leukocytes and Bioinformatic Analysis of Patients with Moyamoya Disease
Authors: Kang Kaijiang , Shen Yuan , Zhang Qian , Lu Jingjing , Ju Yi , Ji Ruijun , Li Na , Wu Jianwei , Yang Bo , Lin Jinxi , Liang Xianhong , Zhang Dong , Zhao Xingquan 
Published: September 2021

Abstract
Background and purpose- MicroRNAs (miRNAs) in exosomes had been implicated differentially expressed in MMD patients, but the miRNAs expression in circulating leukocytes remains unclear. This study was investigated on the differential expression of miRNAs in peripheral leukocytes between MMD patients and healthy adults, and among patients with subtypes of MMD.Methods- A total of 30 patients with MMD and 10 healthy adults were enrolled in a stroke center from October 2017 to December 2018. The gene microarray was used to detect the differential expression profiles of miRNA in leukocytes between MMD patients and controls, and the differentially expressed miRNAs were verified by the method of real-time PCR. The Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) were used to explore the key signaling pathways and possible pathogenesis of MMD.Results- The microarray results showed 12 differentially expressed miRNAs in leukocytes of MMD patients compared with controls (fold change > 2.0 and P < 0.05), of which 7 miRNAs were up-regulated (miRNA-142-5p, miRNA-29b-3p, miRNA-424-5p, MiRNA-582-5p, miRNA-6807-5p, miRNA-142-3p, miRNA-340-5p), and 5 miRNAs were down-regulated (miRNA-144-3p, miRNA-144-5p, miRNA-451a, miRNA-486-5p, miRNA-363-3p). The real-time PCR confirmed 7 differentially expressed miRNAs (P<0.05), of which 4 miRNAs (miRNA-29b-3p, miRNA-142-3p, miRNA-340-5p, miRNA-582-5p) were up-regulated, and 3 miRNAs (miRNA-363-3p, miRNA-451a and miRNA-486-5p) were down-regulated. Both GO and KEGG analysis suggested that the Wnt signaling pathway may be involved in the pathogenesis of MMD. In addition, miRNAs were also differentially expressed among patients with subtypes of MMD. Conclusion- This study indicated that miRNAs are differentially expressed in peripheral leukocytes between MMD patients and healthy adults, and among patients with subtypes of MMD. The Wnt signaling pathway is probably involved in the pathogenesis of MMD.
Therapeutic Potential of miR-29a in Breast Cancer Patients with type 2 Diabetes and its Regulatory Mechanism
Authors: li zhihua , Hu Yang-yang , Luan Wen-jing , Li Cong , Xiong Qiu-yun , Huang Kedi , Min Liangliang , cheng Lu 
Published: September 2021

Abstract
MicroRNAs (miRNAs) acting as tumour suppressors or oncogenes (oncomiRs), are a promising new focus of targeted therapeutics for cancer. Diabetes preexisted in approximately 16% of breast cancer patients. This study mainly aims to investigate the therapeutic potential of miR-29a in breast cancer patients with type 2 diabetes (BDM) and its regulatory mechanism. Our results indicated that miR-29a is upregulated in BDM patients, which was correlated with worse prognosis. In human breast cancer cells, miR-29a knockdown reduced cell proliferation, cells migration and invasion in BDM conditions (25 mmol/L glucose + 25 nmol/L insulin). In xenograft mouse model for BDM, miR-29a knockdown suppresses MDA-MB-231 cells tumourigenesis and metastasis. Finally,we demonstrated that miR-29a promoted cell growth and metastasis of BDM via targeting of SIRT1.Collectively, our findings indicated that anti-miR-29a inhibited cell proliferation and invasion in BDM by targeted SIRT1, anti-miR-29a may represent a novel therapeutic approach for the management of BDM patients.
Factors of Dental Hygienists' Experiences Related to the Elderly that Affect the Perception of Human Rights Violations Against the Elderly: A Cross Sectional Study
Authors: Jang Kyeung-Ae , Kim Yu-Rin 
Published: September 2021

Abstract
Background: Korean society has become an elderly society at an unprecedented rate, and the social and economic problems emerging in such a society are very serious. Good oral health is important for older people’s wellbeing and quality of life, and is essential for healthy ageing. Therefore, the purpose of this study is to examine the effect of the elderly-related experiences of dental hygienists in charge of oral health on human rights violations against the elderly. Methods: This study was conducted through an IRB review at Silla University in Korea (No. 1041449-202012-HR-001). For about a month from February 1, 2021, the survey questionnaire was distributed to dental hygienists living in South Korea, and 153 people who accomplished and sent back the questionnaires were selected as the final subjects. Polyserial correlation analysis was performed to confirm the relevance of each factor, and multiple regression analysis was performed to identify the factors of elderly-related experiences that affect the perception of human rights violations against the elderly.Results: All three experiences related to the elderly were highly related to the perception of violations of financial human rights of the elderly (p<0.01). In addition, the experience of education related to the elderly showed a high perception of psychological violation of the human rights of the elderly (R=0.405, p<0.01), and the experience of volunteer activities related to the elderly showed a high perception of neglectful violations of human rights of the elderly (R=0.277, p<0.01). Conclusion: At this point in time when the number of the elderly is increasing, dental hygienists have to focus on specialized oral health management for the elderly that respects their human rights.
Interferon Regulatory Factor-1 Regulates Cisplatin-Induced Apoptosis and Autophagy in A549 Lung Cancer Cells
Authors: zhang lemeng , Cheng Tianli , Yang Hua , Chen Jianhua , Wen Xiaoping , Jiang Zhou , Yi Huihuang , Luo Yongzhong 
Published: September 2021

Abstract
Purpose: This study aimed to investigate the expression and function of interferon regulatory factor-1 (IRF-1) in non-small cell lung cancer (NSCLC). Methods: IRF-1 expression and its prognostic value were investigated through bioinformatic analysis. The protein expression levels of IRF-1, cleaved caspase-3, and LC3-I/II were analyzed by western blotting. A lentiviral vector was used to overexpress or knockdown IRF-1 in vitro. Mitochondrial membrane potential (MMP) and reactive oxygen species (ROS) were analyzed by JC-1 and DCFH-DA staining, respectively. ATP, SOD, MDA, cell viability, LDH release, and caspase-3 activity were evaluated using commercial kits. Results: Compared to the levels in normal tissues, IRF-1 expression was significantly lower in lung cancer tissues and was a prognostic factor for NSCLC. Cisplatin treatment induced IRF-1 activation, ROS production, ATP depletion, SOD consumption, and MDA accumulation in A549 lung cancer cells. IRF-1 overexpression promoted mitochondrial depolarization, oxidative stress, and apoptotic cell death and inhibited autophagy in A549 cells, and these effects could be reversed by IRF-1 knockdown. Conclusion: These data suggest that IRF-1 regulates apoptosis, autophagy and oxidative stress, which might be served as a potential target for increasing chemotherapy sensitivity of lung cancer.
Multi-body Dynamic Modelling and Error Analysis of Planar Flexible Multilink Mechanism Considering Clearance and Thermal-mechanical Coupling Effect of the Crankshaft-bearing Structure
Authors: Long Hongfan , Han Zhao , Jiang Shuyun , Zheng Enlai , Zhang Yongnian , Zhu Yue , Wang Yongjian 
Published: September 2021

Abstract
In order to study the dynamic position accuracy of bottom dead point (BDP) for multilink high-speed precision presses (MHSPPs), it’s essential to develop a dynamic model of planar multilink mechanism with clearance and spindle-bearing structure. Traditional models always neglect the effect of thermal characteristics of spindle-bearing structure, which reduces the prediction accuracy of dynamic model for multilink transmission mechanisms. To overcome the shortcomings of the previous models, a thermal network model (TNM) of the crankshaft-bearing system is established firstly considering the effects of thermal contact resistance and variable stiffness of bearing concerning the temperature rise. Then, dynamic model of the crankshaft-bearing system is built through the finite element method, which includes rigid disk, Timoshenko beam and quasi-statics model of ACBB. On this basis, an improved dynamic model of planar flexible multilink mechanism with clearance considering the thermal-mechanical coupling effect of the crankshaft-bearing structure is developed and the corresponding dynamic error dimension chain between slider and crankshaft is constructed in this work. Compared to the simulation from traditional models, the simulated slider’s BPD position error from the improved model agrees better with experimental data, which verifies the correctness of the proposed model. It’s demonstrated that the punching force and thermally induced variable stiffness of bearing lead to a significant increase of slider’s BDP position error, which reduces the machining precision of MHSPP. Furthermore, the influence of crankshaft speed, contact angle of bearing and clearance size on the slider’s BDP position error is also investigated.
NIMA-Related Kinase 6 as an Effective Target Inhibits the Hepatocarcinogenesis and Progression of Hepatocellular Carcinoma
Authors: Zhang Hao , Li Bo 
Published: September 2021

Abstract
BackgroundNever in Mitosis Gene A-Related Kinase (NEK), a member of the cell cycle-dependent protein kinase, encodes a serine/threonine protein kinase involved in mitosis of the G2-M transition period. Eleven NEKs have been identified, and NEK6 is one of them. It has been confirmed that NEK6 is over-expressed in some tumor cell lines. The following study aims to explore the expression of NEK6 in hepatocellular carcinoma (HCC) and the correlation between NEK6 expression and clinical features.MethodsLiver tissues of 79 HCC patients and other patients were obtained during various liver surgeries. Total RNA from these tissue samples was extracted and QPCR was adopted to detect positive expression of NEK6. The correlation between NEK6 expression and the clinical characteristics of HCC was analyzed. The expression of NEK6 in different cell lines (LO-2, HepG2, Li-7, Huh-7, and BEL-7402) was quantified via QPCR. Scratch assay, Transwell assay, and tumor-formation assay in nude mice were used to evaluate the effects of NEK6 on the HCC progression in vitro and in vivo.ResultThe expression of NEK6 was up-regulated in HCC tissue compared with other tissues. The proportion of hepatitis B virus infection in the Nek6 Overexpression group was higher than the Control group (P=0.045). The multiple tumors and bigger tumors (>5 cm) seemed more common in the NEK6 overexpression group (P=0.018, and P=0.037, respectively). Further analysis showed that the overexpression of NEK6 was correlated with hepatitis B virus infection and tumor diameter (P=0.045; P=0.038). The 3-year disease-free survival (DFS) and overall survival (OS) of patients in the NEK6 overexpression group were 14.1% and 33.4%, which were worse than the control group. Tumor size over 5 cm and portal vein invasion were risk factors for both DFS and OS. Nek6 overexpression did not seem to affect the prognosis as a risk factor. Among the human cell lines, the expression of NEK6 was higher in Li-7 cells and HepG2 cells. The migration and invasion capabilities of Li-7 and HepG2 cells were suppressed when the NEK6 expression was down-regulated. The xenograft tumor weight was signiﬁcantly lighter in the shNEK6 group (P < 0.05). ConclusionThe results from the study demonstrated that the expression level of NEK6 was up-regulated in HCC and correlated with the HCC progression, suggesting it might be a clinically valuable biomarker and serve as a potential therapeutic target for treating HCC.
Full reconstruction of simplicial complexes from binary time-series data
Authors: Wang Huan , Ma Chuang , Chen Han-Shuang , Lai Ying-Cheng , Zhang Hai-Feng 
Published: September 2021

Abstract
Previous efforts on data-based reconstruction focused on complex networks with pairwise or two-body interactions. There is a growing interest in networks with high-order or many-body interactions, raising the need to reconstruct such networks based on observational data. We develop a general framework combining statistical inference and expectation maximization to fully reconstruct 2-simplicial complexes with two- and three-body interactions based on binary time-series data from social contagion dynamics. We further articulate a two-step scheme to improve the reconstruction accuracy while significantly reducing the computational load. Through synthetic and real-world 2-simplicial complexes, we validate the framework by showing that all the connections can be faithfully identified and the full topology of the 2-simplicial complexes can be inferred. The effects of noisy data or stochastic disturbance are studied, demonstrating the robustness of the proposed framework.
MiR-574-5P, miR-1827, And miR-4429 As Potential Biomarkers For Schizophrenia
Authors: Davarinejad Omran , Najafi Sajad , Zhaleh Hossein , Golmohammadi Farzaneh , Radmehr Farnaz , Alikhani Mostafa , Moghadam Reza Heidari , Rahmati Yazdan 
Published: September 2021

Abstract
Schizophrenia is a severe chronic debilitating disorder with millions of affected individuals. Lack of a reliable mollecular diagnostic invokes the identification of novel biomarkers. To elucidate the molecular basis of the disease, two mRNA expression arrays including GSE93987 and GSE38485, and one miRNA array, GSE54914, were downloaded from GEO, and meta-analysis was performed for mRNA expression arrays by employment of metaDE package. By WGCNA package, we performed network analysis for both mRNA expression arrays separately. Then, we made protein-protein interaction network for significant modules. Limma package was employed to analyze the miRNA array and dysregulated miRNAs (DEMs) were identified. Using genes of significant modules and DEMs, a mRNA-miRNA network was constructed and hub genes and miRNAs were identified. To confirm the dysregulation of genes, expression values were evaluated by available datasets including GEO series GSE62333, GSE93987, and GSE38485. The ability of the detected hub miRNAs to discriminate Schizophrenia from healthy controls was evaluated by assessing the receiver-operating curve. Finally, by performing Real-Time PCR, the expression level of genes and miRNAs were evaluated in 40 Schizophrenia patients compared with healthy controls. The results confirmed dysregulation of hsa-miR-574-5P, hsa-miR-1827, hsa-miR-4429, CREBRF, ARPP19, TGFBR2, and YWHAZ in blood samples of schizophrenia patients.
Predictive Value of Decreased Serum Estradiol Levels Following Human Chorionic Gonadotropin Trigger For Unexpected Ovulation During In Vitro Fertilization Cycle of Patients With Poor Ovarian Response
Authors: Huang Qianwen , Zhang Qianyu , Sun Li , Tang Ting , Liu Wenjuan , Zhang Jie , Liu Fenghua 
Published: September 2021

Abstract
Background

Unexpected spontaneous ovulation typically occurs in patients with poor ovarian response (POR); however, reliable predictors are lacking. We analyzed explore the predictive value of decreased serum estradiol (E2) levels on the day after human chorionic gonadotropin (hCG) trigger during in vitro fertilization (IVF) cycles.
Methods

We retrospectively analyzed clinical data (n = 978) in IVF cycles between 2017 and 2020. According to decreased ratios of E2 levels (△E2%) between day 1 and day 0 of hCG trigger, patients were divided into: ≤10% (group A), 10–20% (group B), and ≥ 20% (group C). Basic characteristics and laboratory data were compared using analysis of variance or Mann–Whitney U-test. Logistic regression and receiver operating characteristic (ROC) curve analyses were used to assess the relationship between △E2% and spontaneous unexpected ovulation. Fresh embryo transfer cycles in groups A, B, and C were subgrouped as A1, B1, and C1, respectively; clinical outcomes were compared.
Results

Mean E2 and luteinizing hormone (LH) levels, ratio of premature LH peak and high progesterone levels, time interval between hCG trigger and oocyte retrieval, number of follicles ≥ 11 mm in diameter, and dose regimen of gonadotropin among the three groups showed significant differences (P < 0.05). After adjusting for relevant confounders, likelihood ratios of unexpected ovulation in groups B and C were 2.96 (95 % confidence interval [CI]: 0.64–13.70), and 11.74 (95 % CI: 3.09–44.62), respectively, compared with that in group A. The test for trend was significant before and after correction (P for trend < 0.05). On combining premature LH peak (> 10 mIU/mL) or high progesterone level (> 1.0 ng/mL) on the day of hCG, area under ROC curve (AUC) was 0.849 (95 % CI, 0.764–0.934, P < 0.05). However, when we only considered △E2%, AUC was 0.738 (95 % CI. 0.562–0.914, P < 0.05). Rates of embryo implantation, clinical pregnancy, live birth in fresh embryo transfer cycle, and early abortion among the three subgroups did not differ significantly (P > 0.05).
Conclusions

E2 levels on the day after hCG trigger are valuable for predicting unexpected ovulation. However, their decreased levels do not directly affect clinical pregnancy outcomes.
GnRH antagonist weakens endometrial stromal cells growth ability by decreasing c-kit receptor expression
Authors: Xu Ding-Fei , Liu Pei-Pei , Fan Lu , Xie Qi , Zhang Zhi-Qin , Tan Jun , Wu Qiong-Fang , Wang Li-Qun 
Published: September 2021

Abstract
Background
Several surveys have reported that patients treated with gonadotropin-releasing hormone antagonist (GnRH-ant) protocol showed a significantly lower rate of implantation and clinical pregnancy compared to GnRH agonist (GnRH-a) protocol during in vitro fertilization-fresh embryo transfer. Subsequent studies imputed this poor outcome to the negative effects of GnRH-ant on endometrial receptive. However, the mechanisms were not fully understood.
Methods
The clinical data of 2815 patients undergoing fresh embryo transfer in our center were analyzed. Human endometrial stromal cells (ESCs) from healthy women undergoing elective pregnancy termination of a normal pregnancy at 8–10 weeks gestation were treated with GnRH-analogs or imatinib (c-kit receptor inhibitor).
Results
The clinical data showed that the endometrial thickness on HCG Day were significantly lower in GnRH-ant group. Although no difference of embryo quality in these two groups, GnRH-ant group showed remarkably decreased rate of HCG positive, embryo implantation and pregnancy. Moreover, GnRH-ant significantly reduced the proliferation and induced the apoptosis of ESCs. Furthermore, the expression and activation of c-kit receptor, which played pivotal roles during embryo implantation, were observably decreased by GnRH-ant. Inhibiting the activation of c-kit by imatinib remarkably suppressed the proliferation and promoted the apoptosis of ESCs. Additionally, the phosphorylation of AKT and expression of Cyclin D1, which were closely related with cellular growth, were distinctly lessened after treating with imatinib.
Conclusions
In summary, our study showed that GnRH-ant weakened the activization of c-kit receptor by decreasing its expression, causing the impaired growth ability of ESCs. Our findings provided a new insight into the effects of GnRH-ant on endometrium.
Residential environment in relation to self-report of respiratory and asthma symptoms among primary school children in a high-polluted urban area
Authors: Apichainan Nawarat , Norkaew Saowanee , Taneepanichskul Nutta 
Published: September 2021

Abstract
Respiratory disease and its complication are the cause of children deaths worldwide every year. Several epidemiological studies pointed out an association between quality of residential in inner city and risk of children health. Few studies had been focused on respiratory and asthma symptoms in high-polluted urban area in low to middle income countries. We conducted a cross-sectional study to investigate the association between residential environments and respiratory symptoms including asthma among 658 primary school children aged between 6 to 10 years who have been living in high-polluted urban area of Bangkok Thailand. The International Study of Asthma and Allergies in Childhood (ISAAC) questionnaire was modified and used for symptom self-report during the past 12 months. Binary logistic regression model was performed to find the associations. The results showed that living near garment and clothing shop is associated with shortness of breath (AOR = 1.846; 95%CI 1.034, 3.297). Vectors in home is related to dry cough at night (AOR = 1.505; 95%CI 1.052, 2.153) and phlegm (AOR = 1.414; 95%CI 1.014, 1.973). Wall dampness is increased odd of having wheezing or whistling (asthma) in the chest (AOR = 1.921; 95%CI 1.141, 3.235). Housing quality and living environments to reduce respiratory and asthma symptoms among urban children must be improved to address these risks.
Determination of Probability of Causative Pathogen in Infectious Keratitis Using Deep Learning Algorithm of Slit-Lamp Images
Authors: Koyama Ayumi , Miyazaki Dai , Nakagawa Yuji , Ayatsuka Yuji , Miyake Hitomi , Ehara Fumie , Sasaki Shin-ichi , Shimizu Yumiko , Inoue Yoshitsugu 
Published: September 2021

Abstract
Corneal opacities are an important cause of blindness, and its major etiology is infectious keratitis. Slit-lamp examinations are commonly used to determine the causative pathogen; however, their diagnostic accuracy is low even for experienced ophthalmologists. To characterize the “face” of an infected cornea, we have adapted a deep learning architecture used for facial recognition and applied it to determine a probability score for a specific pathogen causing keratitis. To record the diverse features and mitigate the uncertainty, batches of probability scores of 4 serial images taken from many angles or fluorescence staining were learned for score and decision level fusion using a gradient boosting decision tree. A total of 4306 slit-lamp images and 312 images obtained by internet publications on keratitis by bacteria, fungi, acanthamoeba, and herpes simplex virus (HSV) were studied. The created algorithm had a high overall accuracy of diagnosis, e.g., the accuracy/area under the curve (AUC) for acanthamoeba was 97.9%/0.995, bacteria was 90.7%/0.963, fungi was 95.0%/0.975, and HSV was 92.3%/0.946, by group K-fold validation, and it was robust to even the low resolution web images. We suggest that our hybrid deep learning-based algorithm be used as a simple and accurate method for computer-assisted diagnosis of infectious keratitis.