bluuebunny/crossref_metadata_2025_split huggingface dataset:
Intra-Areal Visual Topography in Primate Brains Mapped with Probabilistic Tractography of Diffusion-Weighted Imaging
Authors: Tang-Wright K , Smith J E T , Bridge H , Miller K L , Dyrby T B , Ahmed B , Reislev N L , Sallet J , Parker A J , Krug K 
Published: September 2021

Abstract
Noninvasive diffusion-weighted magnetic resonance imaging (dMRI) can be used to map the neural connectivity between distinct areas in the intact brain, but the standard resolution achieved fundamentally limits the sensitivity of such maps. We investigated the sensitivity and specificity of high-resolution postmortem dMRI and probabilistic tractography in rhesus macaque brains to produce retinotopic maps of the lateral geniculate nucleus (LGN) and extrastriate cortical visual area V5/MT based on their topographic connections with the previously established functional retinotopic map of primary visual cortex (V1). We also replicated the differential connectivity of magnocellular and parvocellular LGN compartments with V1 across visual field positions. Predicted topographic maps based on dMRI data largely matched the established retinotopy of both LGN and V5/MT. Furthermore, tractography based on in vivo dMRI data from the same macaque brains acquired at standard field strength (3T) yielded comparable topographic maps in many cases. We conclude that tractography based on dMRI is sensitive enough to reveal the intrinsic organization of ordered connections between topographically organized neural structures and their resultant functional organization.
Genome Sequencing Identifies Previously Unrecognized Klebsiella pneumoniae Outbreaks in Neonatal Intensive Care Units in the Philippines
Authors: Carlos Celia C , Masim Melissa Ana L , Lagrada Marietta L , Gayeta June M , Macaranas Polle Krystle V , Sia Sonia B , Facun Maria Adelina M , Palarca Janziel Fiel C , Olorosa Agnettah M , Cueno Gicell Anne C , Abrudan Monica , Abudahab Khalil , Argimón Silvia , Kekre Mihir , Underwood Anthony , Stelling John , Aanensen David M ,   NIHR Global Health Research Unit on Genomic Surveillance of Antimicrobial Resistance, Harste Harry , Muddyman Dawn , Taylor Ben , Wheeler Nicole , David Sophia , Donado-Godoy Pilar , Bernal Johan Fabian , Arevalo Alejandra , Valencia Maria Fernanda , Osma Castro Erik C D , Ravikumar K L , Nagaraj Geetha , Shamanna Varun , Govindan Vandana , Prabhu Akshata , Sravani D , Shincy M R , Rose Steffimole , Ravishankar K N , Okeke Iruka N , Oaikhena Anderson O , Afolayan Ayorinde O , Ajiboye Jolaade J , Ewomazino Odih Erkison , Molloy Ali , Vegvari Carolin 
Published: September 2021

Abstract

Background
Klebsiella pneumoniae is a critically important pathogen in the Philippines. Isolates are commonly resistant to at least 2 classes of antibiotics, yet mechanisms and spread of its resistance are not well studied.

Methods
A retrospective sequencing survey was performed on carbapenem-, extended spectrum beta-lactam-, and cephalosporin-resistant Klebsiella pneumoniae isolated at 20 antimicrobial resistance (AMR) surveillance sentinel sites from 2015 through 2017. We characterized 259 isolates using biochemical methods, antimicrobial susceptibility testing, and whole-genome sequencing (WGS). Known AMR mechanisms were identified. Potential outbreaks were investigated by detecting clusters from epidemiologic, phenotypic, and genome-derived data.

Results
Prevalent AMR mechanisms detected include blaCTX-M-15 (76.8%) and blaNDM-1 (37.5%). An epidemic IncFII(Yp) plasmid carrying blaNDM-1 was also detected in 46 isolates from 6 sentinel sites and 14 different sequence types (STs). This plasmid was also identified as the main vehicle of carbapenem resistance in 2 previously unrecognized local outbreaks of ST348 and ST283 at 2 different sentinel sites. A third local outbreak of ST397 was also identified but without the IncFII(Yp) plasmid. Isolates in each outbreak site showed identical STs and K- and O-loci, and similar resistance profiles and AMR genes. All outbreak isolates were collected from blood of children aged &lt; 1 year.

Conclusion
WGS provided a better understanding of the epidemiology of multidrug resistant Klebsiella in the Philippines, which was not possible with only phenotypic and epidemiologic data. The identification of 3 previously unrecognized Klebsiella outbreaks highlights the utility of WGS in outbreak detection, as well as its importance in public health and in implementing infection control programs.
Norovirus Outbreaks in Long-term Care Facilities in the United States, 2009–2018: A Decade of Surveillance
Authors: Calderwood Laura E , Wikswo Mary E , Mattison Claire P , Kambhampati Anita K , Balachandran Neha , Vinjé Jan , Barclay Leslie , Hall Aron J , Parashar Umesh , Mirza Sara A 
Published: September 2021

Abstract

Background
In the United States, norovirus is the leading cause of healthcare-associated gastroenteritis outbreaks. To inform prevention efforts, we describe the epidemiology of norovirus outbreaks in long-term care facilities (LTCFs).

Methods
The Centers for Disease Control and Prevention (CDC) collect epidemiologic and laboratory data on norovirus outbreaks from US health departments through the National Outbreak Reporting System (NORS) and CaliciNet. Reports from both systems were merged, and norovirus outbreaks in nursing homes, assisted living, and other LTCFs occurring in 2009–2018 were analyzed. Data from the Centers for Medicare and Medicaid Services and the National Center for Health Statistics were used to estimate state LTCF counts.

Results
During 2009–2018, 50 states, Washington D.C., and Puerto Rico reported 13 092 norovirus outbreaks and 416 284 outbreak-associated cases in LTCFs. Participation in NORS and CaliciNet increased from 2009 to 2014 and median reporting of LTCF norovirus outbreaks stabilized at 4.1 outbreaks per 100 LTCFs (interquartile range [IQR]: 1.0–7.1) annually since 2014. Most outbreaks were spread via person-to-person transmission (90.4%), and 75% occurred during December–March. Genogroup was reported for 7292 outbreaks with 862 (11.8%) positive for GI and 6370 (87.3%) for GII. Among 4425 GII outbreaks with typing data, 3618 (81.8%) were GII.4. LTCF residents had higher attack rates than staff (median 29.0% vs 10.9%; P &lt; .001). For every 1000 cases, there were 21.6 hospitalizations and 2.3 deaths.

Conclusions
LTCFs have a high burden of norovirus outbreaks. Most LTCF norovirus outbreaks occurred during winter months and were spread person-to-person. Outbreak surveillance can inform development of interventions for this vulnerable population, such as vaccines targeting GII.4 norovirus strains.
Toward Understanding Death
Authors: Dolan Samantha B , Mahon Barbara E , Dowell Scott F , Zaidi Anita 
Published: September 2021

Abstract
Evidence-based approaches to preventing child death require evidence; without data on common causes of child mortality, taking effective action to prevent these deaths is difficult at best. Minimally invasive tissue sampling (MITS) is a potentially powerful, but nascent, technique to obtain gold standard information on causes of death. The Gates Foundation committed to further establishing the methodology and obtain the highest quality information on the major causes of death for children under 5 years. In 2018, the MITS Surveillance Alliance was launched to implement, refine, and enhance the use of MITS across high mortality settings. The Alliance and its members have contributed to some remarkable opportunities to improve mortality surveillance, and we have only just begun to understand the possibilities on larger scales. This supplement showcases studies conducted by MITS Surveillance Alliance members and represents a significant contribution to the cause-of-death literature from high mortality settings.
Face Identification System Based on Synthesizing Realistic Image using Edge-Aided GANs
Authors: Majidpour Jafar , Kais Jameel Samer , Anwar Qadir Jihad 
Published: September 2021

Abstract
Presently, facial image recognition via a thermal camera is a critical phase in numerous fields. Systems using thermal facial images suffer from numerous problems in face identification. In this paper, a model Edge-Aided Generative Adversarial Network (EA-GAN) is introduced to overcome the difficulties of thermal face identification by synthesizing a visible faces image from the thermal version. To enhance the performance of the Conditional Generative Adversarial Network (CGAN) model for the create realistic face images, the edge information extracted from the thermal image has been used as input, thus lead to improving overall the system's achievement. Moreover, a new model is presented in the present work for face identification by integrating two Convolutional Neural Networks (CNN) to achieve high and rapid accuracy rates. Based on the experiments on the Carl dataset for faces, it is indicated that EA-GAN can synthesize visually comfortable and identity-preserving faces; thus, better performance is achieved in comparison with the state-of-the-art approaches for thermal facial identification.
Early warning indicators of COVID-19 burden for a prosilient European pandemic response
Authors: Beatrice Farrugia , Calleja Neville 
Published: September 2021

Abstract

Background
The European Union has been criticized for responding to the COVID-19 pandemic in a reactive, rather than prosilient manner. For the EU bloc to be prosilient, it needs to have the right early warning indicators to allow short-term healthcare system preparedness and agile planning of the public health response.

Method
The association of COVID-19 disease burden, as measured by mortality (COVID-19 and all-cause), hospital and ICU occupancy, with incidence rate (IR), total positivity rate (TPR) and adjusted TPR as proposed by Vong and Kakkar, was investigated using Poisson regression analysis. This was carried out using both real-time data and time lags of up to 8 weeks to identify potential for early warning of spikes in disease burden. ECDC weekly figures for these indicators were used, and the analysis was repeated for the subset of data after Week 42  of 2020, when the EU Council introduced minimum COVID-19 testing rates.

Results
TPR and IR were noted to be the most predictive of COVID-19 disease burden whilst adjusted TPR applied on weekly data was not associated. TPR behaved better at predicting all-cause mortality in both analyses. The TPR and IR were both best associated with hospital and ICU occupancy and COVID-19 mortality with a short time lag (2–3 weeks in the case of TPR with hospital occupancy and COVID-19 mortality).

Conclusions
Monitoring TPR can provide a 2–3-week warning of a spike in hospital occupancy and COVID-19 mortality. This time, if well utilized, could help health systems save countless lives by mobilising resources.
CDC26 is a key factor in human oocyte aging
Authors: Li Li , Xia Ye , Yang Yang , Zhang Wuwen , Yan Hua , Yin Ping , Li Kai , Chen Yuanyuan , Lu Lu , Tong Guoqing 
Published: September 2021

Abstract

STUDY QUESTION
Is CDC26 a key factor in human oocyte aging?

SUMMARY ANSWER
The lack of CDC26 disrupts the oocytes maturation process, leading to oocyte aging, but these defects could be partially rescued by overexpression of the CDC26 protein.

WHAT IS KNOWN ALREADY
Age-related oocyte aging is the main cause of female fertility decline. In mammalian oocytes, aberrant meiosis can cause chromosomal abnormalities that might lead to infertility and developmental disorders. CDC26 participates in the meiosis process.

STUDY DESIGN, SIZE, DURATION
Differential gene expression in young and old women oocytes were screened by single-cell RNA-seq technology, and the functions of differentially genes were verified on mouse oocytes. Finally, transfection technology was used to evaluate the effect of a differentially expressed gene in rescuing human oocyte from aging.

PARTICIPANTS/MATERIALS, SETTING, METHODS
Discarded human oocytes were collected for single-cell RNA-seq, q-PCR and immunocytochemical analyses to screen for and identify differential gene expression. Female KM mice oocytes were collected for IVM of oocytes, q-PCR and immunocytochemical analyses to delineate the relationships between oocyte aging and differential gene expression. Additionally, recombinant lentiviral vectors encoding CDC26 were transfected into the germinal vesicle oocytes of older women, to investigate the effects of the CDC26 gene expression on oocyte development.

MAIN RESULTS AND THE ROLE OF CHANCE
Many genes were found to be differentially expressed in the oocytes of young versus old patients via RNA-seq technology. CDC26 mRNA and protein levels in aged oocytes were severely decreased, when compared with the levels observed in young oocytes. Moreover, aged oocytes lacking CDC26 were more prone to aneuploidy. These defects in aged oocytes could be partially rescued by overexpression of the CDC26 protein.

LARGE SCALE DATA
N/A.

LIMITATIONS, REASONS FOR CAUTION
Our study delineated key steps in the oocyte aging process by identifying the key role of CDC26 in the progression of oocyte maturation. Future studies are required to address whether other signaling pathways play a role in regulating oocyte maturation via CDC26 and which genes are the direct molecular targets of CDC26.

WIDER IMPLICATIONS OF THE FINDINGS
Our results using in vitro systems for both mouse and human oocyte maturation provide a proof of principle that CDC26 may represent a novel therapeutic approach against maternal aging-related spindle and chromosomal abnormalities.

STUDY FUNDING/COMPETING INTEREST(S)
This work was supported by grants from the National Natural Science Foundation of China (81571442 and 81170571), the outstanding Talent Project of Shanghai Municipal Commission of Health (XBR2011067) and Clinical Research and Cultivation Project in Shanghai Municipal Hospitals (SHDC12019X32). The authors declare no conflict of interest.
Application of Homogeneous Liquid–Liquid Microextraction With Switchable Hydrophilicity Solvents to the Determination of MDMA, MDA and NBOMes in Postmortem Blood Samples
Authors: Scheid Camila , Eller Sarah , Oenning Anderson Luiz , Carasek Eduardo , Merib Josias , de Oliveira Tiago Franco 
Published: September 2021

Abstract
Synthetic drugs for recreational purposes are in constant evolution, and their consumption promotes a significant increase in intoxication cases, resulting in damaging public health. The development of analytical methodologies to confirm the consumption of illicit drugs in biological matrices is required for the control of these substances. This work exploited the development of an extraction method based on homogenous liquid–liquid microextraction with switchable hydrophilicity solvent (SHS) as extraction phase for the determination of the synthetic drugs 3,4-methylenedioxymethamphetamine, 3,4-methylenedioxyamphetamine and N-methoxybenzyl-methoxyphenylethylamine derivates (25B, 25C and 25I) in postmortem blood, followed by liquid chromatography coupled to mass spectrometry in tandem. The optimized sample preparation conditions consisted of using 250 µL of ZnSO4 10% and 50 µL of NaOH 1 mol/L in the protein precipitation step; N,N-dimethylcyclohexylamine was used as SHS, 650 μL of a mixture of SHS:HCl 6 mol/L (1:1 v/v), 500 μL of whole blood, 500 μL of NaOH 10 mol/L and 1 min of extraction time. The proposed method was validated, providing determination coefficients higher than 0.99 for all analytes; limit of detection and limit of quantitation ranged from 0.1 to 10 ng/mL; intra-run precision from 2.16% to 9.19%; inter-run precision from 2.39% to 9.59%; bias from 93.57% to 115.71% and matrix effects from 28.94% to 51.54%. The developed method was successfully applied to four authentic postmortem blood samples from synthetic drugs users, and it was found to be reliable with good selectivity.
Interference Efficiency and Effects of Bacterium-mediated RNAi in the Fall Armyworm (Lepidoptera: Noctuidae)
Authors: Wan Xiao-Shuang , Shi Min-Rui , Xu Jin , Liu Jian-Hong , Ye Hui 
Published: September 2021

Abstract
RNAi is an effective tool for gene function analysis and a promising strategy to provide environmentally friendly control approaches for pathogens and pests. Recent studies support the utility of bacterium-mediated RNAi as a cost-effective method for gene function study and a suitable externally applied delivery mechanism for pest control. Here, we developed a bacterium-mediated RNAi system in Spodoptera frugiperda based on four target genes, specifically, Chitinase (Sf-CHI), Chitin synthase B (Sf-CHSB), Sugar transporter SWEET1 (Sf-ST), and Hemolin (Sf-HEM). RNAi conducted by feeding larvae with bacteria expressing dsRNAs of target genes or injecting pupae and adults with bacterially synthesized dsRNA induced silencing of target genes and resulted in significant negative effects on growth and survival of S. frugiperda. However, RNAi efficiency and effects were variable among different target genes and dsRNA delivery methods. Injection of pupae with dsCHI and dsCHSB induced a significant increase in wing malformation in adults, suggesting that precise regulation of chitin digestion and synthesis is crucial during wing formation. Injection of female moths with dsHEM resulted in lower mating, fecundity, and egg hatching, signifying a critical role of Sf-HEM in the process of egg production and/or embryo development. Our collective results demonstrate that bacterium-mediated RNAi presents an alternative technique for gene function study in S. frugiperda and a potentially effective strategy for control of this pest, and that Sf-CHI, Sf-CHSB, Sf-ST, and Sf-HEM encoding genes can be potent targets.
Arabidopsis annexin 5 is involved in maintenance of pollen membrane integrity and permeability
Authors: Lichocka Małgorzata , Krzymowska Magdalena , Górecka Magdalena , Hennig Jacek 
Published: September 2021

Abstract
In Arabidopsis, a dry stigma surface enables a gradual hydration of pollen grains by a controlled release of water. Occasionally the grains may be exposed to extreme precipitations that cause rapid water influx and swelling, eventually leading to pollen membrane rupture. In metazoans, calcium- and phospholipid-binding proteins, referred to as annexins, participate in the repair of plasma membrane damages. It remains unclear, however, how this process is conducted in plants. Here, we examined whether plant annexin 5 (ANN5), the most abundant member of the annexin family in pollen, is involved in the restoration of pollen membrane integrity. We analyzed the cellular dynamics of ANN5 in pollen grains undergoing hydration in favorable or stress conditions. We observed a transient association of ANN5 with the pollen membrane during in vitro hydration that did not occur in the pollen grains being hydrated on the stigma. To simulate a rainfall, we performed spraying of the pollinated stigma with deionized water that induced ANN5 accumulation at the pollen membrane. Interestingly, calcium or magnesium application affected pollen membrane properties differently, causing rupture or shrinkage of pollen membrane, respectively. Both treatments, however, induced ANN5 recruitment to the pollen membrane. Our data suggest a model in which ANN5 is involved in the maintenance of membrane integrity in pollen grains exposed to osmotic or ionic imbalances.
Infrastructuring digital humanities: On relational infrastructure and global reconfiguration of the field
Authors: Pawlicka-Deger Urszula 
Published: September 2021

AbstractHow do the power dynamics of actors in digital knowledge production define the contours of global science and humanities? Where are scholars now in their efforts to improve a networked, global academic system based on the values of equal access to resources, inclusive participation, and the diversity of epistemologies? This article intervenes in these questions by discussing social dimensions of global knowledge infrastructure—connection, standardization, and access—to understand the specification and materialization of global digital humanities (DH). As digital practices expand across the world, the DH community struggles to ensure inclusive participation and equal opportunities in developing the field. This article shows that discrepancies in global DH lie at the root of existing infrastructure inequalities. Drawing on science and technology studies, it then argues that in order to overcome these imbalances, the academic community can seek the ‘infrastructuring’ of DH. Infrastructuring is an analytical concept that shifts attention from ‘structure’ to ‘process’ of co-creation in the vein of participatory design that foregrounds public engagement, shared interest, and long-term relationships with stakeholders to create networks from which equal opportunities and new forms of connections can emerge. This would involve building an inclusive network of unique nodes of local communities on top of the global knowledge infrastructure.
Embedding creativity into digital resources: Improving information discovery for art history
Authors: Kamposiori Christina , Warwick Claire , Mahony Simon 
Published: September 2021

Abstract
Over the past decades, technological advancement and the mass digitization of information resources have led to the development of a range of digital resources for academic scholarship. Understanding the needs of scholars when employing digital resources for their work can lead to the building of digital infrastructure that not only enables access to the required information but also has the potential to transform scholarship through having a positive effect on the whole scholarly workflow. Through this article, we show how the design of digital libraries and resources can be improved to enhance information discovery and use in art history, while also benefiting other key areas of the research process. By employing an ethnographic approach to the study of scholarly practices, we developed a sound understanding of art historians’ behaviour when interacting with information at different stages of the scholarly workflow. Our results show that scholars exhibited highly creative behaviour when conducting core scholarly activities, such as information seeking and use. Yet, the challenges they often encountered showed that there is still more work to be done to improve digital infrastructure and tools for scholarship in the field. Part of this article will focus on the user requirements for designing systems that facilitate discovery, encourage creative use of information, and trigger inspiration.