bluuebunny/crossref_metadata_2025_split huggingface dataset:
Risk factors for rapid progressive neurological deterioration in patients with cervical spondylotic myelopathy
Authors: Zhong Weiyang , Wang Lin , Huang Tianji , Luo Xiaoji 
Published: January 2021

Abstract
Background
The rapid progressive cervical spondylotic myelopathy (rp-CSM) which had a course of CSM less than 1 month and suffered rapidly progressive neurological deterioration had few reports. Therefore, it is important for us to recognize the pathophysiology of CSM especially the rp-CSM. The study aimed to investigate the risk factors for rapidly progressive (rp) neurological deterioration in patients with cervical spondylotic myelopathy (CSM).

Methods
A total of 159 patients were reviewed and divided into an rp-CSM group and a chronic-CSM (c-CSM) group. Various clinical indexes, including age, sex, Japanese Orthopaedic Association (JOA) score, intramedullary MR T2-hyperintensity, congenital/degenerative spinal stenosis, and local type of ossification of the posterior longitudinal ligament (OPLL), were analyzed, and independent risk factors were investigated.

Results
Thirty-four of 159 patients (21.4%) were diagnosed with rp-CSM. All patients were followed up for a mean of 68.56 ± 14.00 months in the rp-CSM group and 62.66 ± 19.95 months in the c-CSM group. No significant difference was found in sex, mean age, smoking and drinking status, diabetes mellitus (DM), hypertension, surgery time, blood loss, JOA score, degenerative spinal stenosis, or OPLL (local). Univariate analyses demonstrated that rp-CSM patients tended to have MR T2-hyperintensity, longer hospital stay, shorter waiting time for surgery, more congenital spinal stenosis, and worse neurological function and to prefer more posterior surgeries than c-CSM patients. A multiple logistic regression analysis showed that congenital spinal stenosis and MR T2-hyperintensity were independently related to the presence of rp-CSM.

Conclusions
MR T2-hyperintensity and congenital spinal stenosis were risk factors for rp-CSM. Although neurological function deteriorates rapidly, early surgical decompression is recommended and can achieve good neurological recovery after surgery, indicating that rp-CSM could be a reversible condition.
Lobectomy for lung cancer with a displaced left B1 + 2 and an anomalous pulmonary vein: a case report
Authors: Sakamoto Shinichi , Takizawa Hiromitsu , Kawakita Naoya , Tangoku Akira 
Published: January 2021

Abstract
Background
A displaced left B1 + 2 accompanied by an anomalous pulmonary vein is a rare condition involving complex structures. There is a risk of unexpected injuries to bronchi and blood vessels when patients with such anomalies undergo surgery for lung cancer.

Case presentation
A 59-year-old male with suspected lung cancer in the left lower lobe was scheduled to undergo surgery. Chest computed tomography revealed a displaced B1 + 2 and hyperlobulation between S1 + 2 and S3, while the interlobar fissure between S1 + 2 and S6 was completely fused. Three-dimensional computed tomography (3D-CT) revealed an anomalous V1 + 2 joining the left inferior pulmonary vein and a branch of the V1 + 2 running between S1 + 2 and S6. We performed left lower lobectomy via video-assisted thoracic surgery, while taking care with the abovementioned anatomical structures. The strategy employed in this operation was to preserve V1 + 2 and confirm the locations of B1 + 2 and B6 when dividing the fissure.

Conclusion
The aim of the surgical procedure performed in this case was to divide the fissure between S1 + 2 and the inferior lobe to reduce the risk of an unexpected bronchial injury. 3D-CT helps surgeons to understand the stereoscopic positional relationships among anatomical structures.
Ultrasonography of kidney and spleen in clinically healthy llamas and alpacas
Authors: Eibl Cassandra , Franz Sonja 
Published: January 2021

Abstract
Background
The ultrasonographic examination technique is a well-established, non-invasive diagnostic tool for diverse conditions in humans and different animal species. The purpose of our study was to describe ultrasonographic localisation, sonographic appearance and dimensions of the kidneys and spleen of clinically healthy llamas and alpacas. Differences between llamas and alpacas and the influence of sex and ages were investigated. Results of this study may aid veterinarians performing ultrasonography in diseased animals and the technique can be used for routine protocol screening.

Results
Ultrasonography was performed in 135 clinically healthy, non-sedated llamas and alpacas. Screening was performed with a 6.6 MHz curve linear transducer with only alcohol as contact medium between the probe and unclipped skin. The kidneys could be imaged from the paralumbar region. The right kidney only was visualized when scanning from the right and the left kidney only from the left. While the left kidney appeared in sagittal view as an oval shape in most llamas and alpacas, in one third of animals the left kidney had a triangular shape. The L-shaped base of the spleen, with its homogeneous, echoic pattern, could be seen craniolateral to the left kidney. Anechoic areas displaying vessels inside the spleen and a thin echoic capsule surrounding the splenic tissue could be differentiated. While sonographic appearances of the examined organs showed no differences between llamas and alpacas, selected dimensions of both of kidney and spleen showed significant differences between species. In terms of age and sex, significant differences in respect of kidney size could be found only in alpacas. Sex seemed to have no influence on kidney and spleen sizes in llamas.

Conclusions
The present study provides species-specific information on ultrasonographic appearance and reference values for kidney and spleen dimensions of clinically healthy llamas and alpacas. Results show differences in organ sizes between llamas and alpacas and in alpacas of different sex and age. The results of this study can be used as references for veterinarians performing ultrasound examinations in diseased animals.
Mechanistic modeling of the SARS-CoV-2 disease map
Authors: Rian Kinza , Esteban-Medina Marina , Hidalgo Marta R. , Çubuk Cankut , Falco Matias M. , Loucera Carlos , Gunyel Devrim , Ostaszewski Marek , Peña-Chilet María , Dopazo Joaquín 
Published: January 2021

AbstractHere we present a web interface that implements a comprehensive mechanistic model of the SARS-CoV-2 disease map. In this framework, the detailed activity of the human signaling circuits related to the viral infection, covering from the entry and replication mechanisms to the downstream consequences as inflammation and antigenic response, can be inferred from gene expression experiments. Moreover, the effect of potential interventions, such as knock-downs, or drug effects (currently the system models the effect of more than 8000 DrugBank drugs) can be studied. This freely available tool not only provides an unprecedentedly detailed view of the mechanisms of viral invasion and the consequences in the cell but has also the potential of becoming an invaluable asset in the search for efficient antiviral treatments.
A blockchain-based service for inviolable presence registration of mobile entities
Authors: Leal Matheus , Pisani Flávia , Endler Markus 
Published: January 2021

AbstractSeveral applications can benefit from recording information about the places a mobile entity visits and the length of time it spends there (e.g., shoppers, employees, buses, portable equipment, autonomous robots). This paper presents our approach to recording spatio-temporal presence information in a secure and inviolable way using a Distributed Ledger Technology. We implemented this solution as a middleware service that uses Complex Event Processing on smartphones to record beacon-smartphone proximity data in a blockchain efficiently. We have built upon the previous version of our service to include access control to the stored information. We analyzed the impact of this addition on the service’s performance and observed that it introduced very little overhead while significantly increasing user privacy. Furthermore, we compared the effect of using different blockchain technologies on overall service performance and characterized scenarios where using either IoTeX or Ethereum can be suitable for this type of application.
A preliminary clinical trial to evaluate 64Cu-NOTA-Trastuzumab as a positron emission tomography imaging agent in patients with breast cancer
Authors: Lee Inki , Lim Ilhan , Byun Byung Hyun , Kim Byung Il , Choi Chang Woon , Woo Sang-Keun , Kim Kwang Il , Lee Kyo Chul , Kang Joo Hyun , Seong Min-Ki , Kim Hyun-Ah , Noh Woo Chul , Lim Sang Moo 
Published: January 2021

Abstract
Background
The purpose of this study was to evaluate both the biodistribution and safety of 64Cu-1,4,7-triazacyclononane-1,4,7-triacetic acid (NOTA)-Trastuzumab, a novel 64Cu-labeled positron emission tomography (PET) tracer for human epidermal growth factor receptor 2 (HER2) in patients with breast cancer.

Methods
PET images at 1, 24, and 48 h after 296 MBq of 64Cu-NOTA-Trastuzumab injection were obtained from seven patients with breast cancer. Both the primary tumors’ and metastatic lesions’ maximum standardized uptake value (SUVmax) was evaluated. The mean SUVmax (SUVmean) was evaluated in the other organs, including the blood pool, liver, kidney, muscle, spleen, bladder, and the lungs, as well as the bones. Moreover, the internal radiation dosimetry was calculated using the OLINDA/EXM software. Safety was assessed based on feedback regarding adverse reactions and safety-related issues within 1 month after 64Cu-NOTA-Trastuzumab administration.

Results
64Cu-NOTA-Trastuzumab PET images showed that the overall SUVmean values in each organ negatively correlated with time. The liver’s average SUVmean values were measured at 5.3 ± 0.7, 4.8 ± 0.6, and 4.4 ± 0.5 on 1 h, 24 h, and 48 h after injection, respectively. The average SUVmean blood values were measured at 13.1 ± 0.9, 9.1 ± 1.2, and 7.1 ± 1.9 on 1 h, 24 h, and 48 h after injection, respectively. The SUVmax of HER2-positive tumors was relatively higher than HER2-negative tumors (8.6 ± 5.1 and 5.2 ± 2.8 on 48 h after injection, respectively). Tumor-to-background ratios were higher in the HER2-positive tumors than in the HER2-negative tumors. No adverse events related to 64Cu-NOTA-Trastuzumab were reported. The calculated effective dose with a 296 MBq injection of 64Cu-NOTA-Trastuzumab was 2.96 mSv. The highest absorbed dose was observed in the liver (0.076 mGy/MBq), followed by the spleen (0.063 mGy/MBq), kidney (0.044 mGy/MBq), and heart wall (0.044 mGy/MBq).

Conclusions
64Cu-NOTA-Trastuzumab showed a specific uptake at the HER2-expressing tumors, thus making it a feasible and safe monitoring tool of HER2 tumor status in patients with breast cancer.

Trial registration

CRIS, KCT0002790. Registered 02 February 2018, https://cris.nih.go.kr
Insulin sensitivity in critically ill patients: are women more insulin resistant?
Authors: Uyttendaele Vincent , Chase J. Geoffrey , Knopp Jennifer L. , Gottlieb Rebecca , Shaw Geoffrey M. , Desaive Thomas 
Published: January 2021

Abstract
Background
Glycaemic control (GC) in intensive care unit is challenging due to significant inter- and intra-patient variability, leading to increased risk of hypoglycaemia. Recent work showed higher insulin resistance in female preterm neonates. This study aims to determine if there are differences in inter- and intra-patient metabolic variability between sexes in adults, to gain in insight into any differences in metabolic response to injury. Any significant difference would suggest GC and randomised trial design should consider sex differences to personalise care.

Methods
Insulin sensitivity (SI) levels and variability are identified from retrospective clinical data for men and women. Data are divided using 6-h blocks to capture metabolic evolution over time. In total, 91 male and 54 female patient GC episodes of minimum 24 h are analysed. Hypothesis testing is used to determine whether differences are significant (P < 0.05), and equivalence testing is used to assess whether these differences can be considered equivalent at a clinical level. Data are assessed for the raw cohort and in 100 Monte Carlo simulations analyses where the number of men and women are equal.

Results
Demographic data between females and males were all similar, including GC outcomes (safety from hypoglycaemia and high (> 50%) time in target band). Females had consistently significantly lower SI levels than males, and this difference was not clinically equivalent. However, metabolic variability between sexes was never significantly different and always clinically equivalent. Thus, inter-patient variability was significantly different between males and females, but intra-patient variability was equivalent.

Conclusion
Given equivalent intra-patient variability and significantly greater insulin resistance, females can receive the same benefit from safe, effective GC as males, but may require higher insulin doses to achieve the same glycaemia. Clinical trials should consider sex differences in protocol design and outcome analyses.
Efficacy and safety of CD22 chimeric antigen receptor (CAR) T cell therapy in patients with B cell malignancies: a protocol for a systematic review and meta-analysis
Authors: Adeel Komal , Fergusson Nathan J. , Shorr Risa , Atkins Harold , Hay Kevin A. 
Published: January 2021

Abstract
Background
Chimeric antigen receptor (CAR) T cell therapy has had great success in treating patients with relapsed or refractory B cell malignancies, with CD19-targeting therapies now approved in many countries. However, a subset of patients fails to respond or relapse after CD19 CAR T cell therapy, in part due to antigen loss, which has prompted the search for alternative antigen targets. CD22 is another antigen found on the surface of B cells. CARs targeting CD22 alone or in combination with other antigens have been investigated in several pre-clinical and clinical trials.
Given the heterogeneity and small size of CAR T cell therapy clinical trials, systematic reviews are needed to evaluate their efficacy and safety. Here, we propose a systematic review of CAR T cell therapies targeting CD22, alone or in combination with other antigen targets, in B cell malignancies.

Methods
We will perform a systematic search of EMBASE, MEDLINE, Web of Science, Cochrane Register of Controlled Trials, clinicaltrials.gov, and the International Clinical Trials Registry Platform. Ongoing and completed clinical trials will be identified and cataloged. Interventional studies investigating CD22 CAR T cells, including various multi-antigen targeting approaches, in patients with relapsed or refractory B cell malignancies will be eligible for inclusion. Only full-text articles, conference abstracts, letters, and case reports will be considered. Our primary outcome will be a complete response, defined as absence of detectable cancer. Secondary outcomes will include adverse events, overall response, minimal residual disease, and relapse, among others. Quality assessment will be performed using a modified Institute of Health Economics tool designed for interventional single-arm studies. We will report a narrative synthesis of clinical studies, presented in tabular format. If appropriate, a meta-analysis will be performed using a random effects model to synthesize results.

Discussion
The results of the proposed review will help inform clinicians, patients, and other stakeholders of the risks and benefits of CD22 CAR T cell therapies. It will identify gaps or inconsistencies in outcome reporting and help to guide future clinical trials investigating CAR T cells.

Systematic review registration
PROSPERO registration number: CRD42020193027
Long-time behavior of solutions for a fractional diffusion problem
Authors: Qi Ailing , Hu Die , Xiang Mingqi 
Published: January 2021

AbstractThis paper deals with the asymptotic behavior of solutions to the initial-boundary value problem of the following fractional p-Kirchhoff equation: $$ u_{t}+M\bigl([u]_{s,p}^{p}\bigr) (-\Delta )_{p}^{s}u+f(x,u)=g(x)\quad \text{in } \Omega \times (0, \infty ), $$

u
t

+
M

(

[
u
]

s
,
p

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)

(
−
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)

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)
=
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(
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)
,
where $\Omega \subset \mathbb{R}^{N}$
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is a bounded domain with Lipschitz boundary, $N>ps$
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, $0< s<1<p$
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is a nondecreasing continuous function, $[u]_{s,p}$

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u
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s
,
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is the Gagliardo seminorm of u, $f:\Omega \times \mathbb{R}\rightarrow \mathbb{R}$
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and $g\in L^{2}(\Omega )$
g
∈

L
2

(
Ω
)
. With general assumptions on f and g, we prove the existence of global attractors in proper spaces. Then, we show that the fractal dimensional of global attractors is infinite provided some conditions are satisfied.
Modified different nonlinearities for weakly coupled systems of semilinear effectively damped waves with different time-dependent coefficients in the dissipation terms
Authors: Mohammed Djaouti Abdelhamid 
Published: January 2021

AbstractWe prove the global existence of small data solution in all spaces of all dimensions $n\geq 1$
n
≥
1
for weakly coupled systems of semilinear effectively damped wave, with different time-dependent coefficients in the dissipation terms. Moreover, we assume that the nonlinearity terms $f(t,u) $
f
(
t
,
u
)
and $g(t,v) $
g
(
t
,
v
)
satisfy some properties of parabolic equations. We study the problem in several classes of regularity.
New quantum boundaries for quantum Simpson’s and quantum Newton’s type inequalities for preinvex functions
Authors: Ali Muhammad Aamir , Abbas Mujahid , Budak Hüseyin , Agarwal Praveen , Murtaza Ghulam , Chu Yu-Ming 
Published: January 2021

AbstractIn this research, we derive two generalized integral identities involving the$q^{\varkappa _{2}}$qϰ2-quantum integrals and quantum numbers, the results are then used to establish some new quantum boundaries for quantum Simpson’s and quantum Newton’s inequalities forq-differentiable preinvex functions. Moreover, we obtain some new and known Simpson’s and Newton’s type inequalities by considering the limit$q\rightarrow 1^{-}$q→1−in the key results of this paper.
Whole microbial community viability is not quantitatively reflected by propidium monoazide sequencing approach
Authors: Wang Ya , Yan Yan , Thompson Kelsey N. , Bae Sena , Accorsi Emma K. , Zhang Yancong , Shen Jiaxian , Vlamakis Hera , Hartmann Erica M. , Huttenhower Curtis 
Published: January 2021

Abstract
Background
High-throughput sequencing provides a powerful window into the structural and functional profiling of microbial communities, but it is unable to characterize only the viable portion of microbial communities at scale. There is as yet not one best solution to this problem. Previous studies have established viability assessments using propidium monoazide (PMA) treatment coupled with downstream molecular profiling (e.g., qPCR or sequencing). While these studies have met with moderate success, most of them focused on the resulting “viable” communities without systematic evaluations of the technique. Here, we present our work to rigorously benchmark “PMA-seq” (PMA treatment followed by 16S rRNA gene amplicon sequencing) for viability assessment in synthetic and realistic microbial communities.

Results
PMA-seq was able to successfully reconstruct simple synthetic communities comprising viable/heat-killed Escherichia coli and Streptococcus sanguinis. However, in realistically complex communities (computer screens, computer mice, soil, and human saliva) with E. coli spike-in controls, PMA-seq did not accurately quantify viability (even relative to variability in amplicon sequencing), with its performance largely affected by community properties such as initial biomass, sample types, and compositional diversity. We then applied this technique to environmental swabs from the Boston subway system. Several taxa differed significantly after PMA treatment, while not all microorganisms responded consistently. To elucidate the “PMA-responsive” microbes, we compared our results with previous PMA-based studies and found that PMA responsiveness varied widely when microbes were sourced from different ecosystems but were reproducible within similar environments across studies.

Conclusions
This study provides a comprehensive evaluation of PMA-seq exploring its quantitative potential in synthetic and complex microbial communities, where the technique was effective for semi-quantitative purposes in simple synthetic communities but provided only qualitative assessments in realistically complex community samples.
Decoding the RNA viromes in rodent lungs provides new insight into the origin and evolutionary patterns of rodent-borne pathogens in Mainland Southeast Asia
Authors: Wu Zhiqiang , Han Yelin , Liu Bo , Li Hongying , Zhu Guangjian , Latinne Alice , Dong Jie , Sun Lilin , Su Haoxiang , Liu Liguo , Du Jiang , Zhou Siyu , Chen Mingxing , Kritiyakan Anamika , Jittapalapong Sathaporn , Chaisiri Kittipong , Buchy Phillipe , Duong Veasna , Yang Jian , Jiang Jinyong , Xu Xiang , Zhou Hongning , Yang Fan , Irwin David M. , Morand Serge , Daszak Peter , Wang Jianwei , Jin Qi 
Published: January 2021

AbstractBackgroundAs the largest group of mammalian species, which are also widely distributed all over the world, rodents are the natural reservoirs for many diverse zoonotic viruses. A comprehensive understanding of the core virome of diverse rodents should therefore assist in efforts to reduce the risk of future emergence or re-emergence of rodent-borne zoonotic pathogens.ResultsThis study aimed to describe the viral range that could be detected in the lungs of rodents from Mainland Southeast Asia. Lung samples were collected from 3284 rodents and insectivores of the orders Rodentia, Scandentia, and Eulipotyphla in eighteen provinces of Thailand, Lao PDR, and Cambodia throughout 2006–2018. Meta-transcriptomic analysis was used to outline the unique spectral characteristics of the mammalian viruses within these lungs and the ecological and genetic imprints of the novel viruses. Many mammalian- or arthropod-related viruses from distinct evolutionary lineages were reported for the first time in these species, and viruses related to known pathogens were characterized for their genomic and evolutionary characteristics, host species, and locations.ConclusionsThese results expand our understanding of the core viromes of rodents and insectivores from Mainland Southeast Asia and suggest that a high diversity of viruses remains to be found in rodent species of this area. These findings, combined with our previous virome data from China, increase our knowledge of the viral community in wildlife and arthropod vectors in emerging disease hotspots of East and Southeast Asia.
Efficacy of alcohol/sugar aqueous biphasic system on partition of bovine serum albumin
Authors: Chow Yin Hui , Sahlini Alagan , Ng Hui-Suan , Lan John Chi-Wei 
Published: January 2021

AbstractThe efficacy of alcohol/sugar aqueous biphasic (ABS) system on protein extraction was investigated. A model protein, bovine serum albumin (BSA), was adopted to evaluate the effects of types and concentration of phase-forming components, protein concentration, and system pH on the protein partition efficiency. The 1-propanol/maltose ABS exhibited an overall better partition efficiency of BSA to the alcohol-rich top phase. A maximum partition coefficient (K) of 20.01 ± 0.05 and recovery yield (Y) of 95.42% ± 0.01% of BSA were achieved with 35% (w/w) 1-propanol/22% (w/w) maltose ABS at pH 5.0 for 10% (w/w) BSA load. The K and Y of BSA in 1-propanol/maltose ABS was slightly improved with the addition of 3% (w/w) of ionic liquid, 1-butyl-3-methylimidazolium bromide ([Bmim]Br) as the adjuvant that could provide protein stabilizing effect. The Fourier Transform Infrared Spectrum (FTIR) analysis revealed that the protein structure remained unaltered upon the separation process.