bluuebunny/crossref_metadata_2025_split huggingface dataset:
Determining the Effect of Hesperidin Supplementation to Japanese Quail Rations on Blood Serum, Tissue Antioxidant Parameters, Intestine Histomorphology, and Feces Microflora
Authors: ÖZBILGIN Abdullah , MOGULKOÇ Mahmut , ERHAN Füsun , ERCAN Nazlı , KARA Kanber 
Published: October 2021

Abstract
This study was conducted to determine the effects of hesperidin, a flavonoid added to quail rations, on blood serum, antioxidant enzymes in tissues, intestinal histomorphology and fecal microflora. In this context, three groups have been created. The first group has been administered basal ration (Group C). The second group (HES1) has been administered basal ration with hesperidin (1g / kg feed). The third group (HES2) has been administered basal ration with additional hesperidin (2g / kg feed). The experiment lasted 35 days. Blood, tissue and feces samples were taken at the end of the experiment. A significant difference has been found in the blood serum in terms of ALT, AST, LDH and Amylase enzymes in the groups with hesperidin compared to the control group (p < 0.05). A significant difference was found in the hesperidine groups in tissue antioxidant GSH, CAT and SOD enzyme parameters compared to the control group (p < 0.05). When looking at the intestinal histomorphology, a significant difference has been found within the test groups in the colon in terms of both villus height and crypt depth, and at villus height in the cecum tissue. As a result, the study's hypothesis has been supported by the fact that hesperidin positively affects the lipid concentration of quail, rump, liver and serum antioxidant enzyme levels, intestinal histomorphology and feces microflora of anaerobe bacteria, particularly Clostridium spp.
Comparative Antibacterial Analysis of the Anthraquinone Compounds Based on the AIM Theory and Molecular Docking Analysis
Authors: Qi Yanjiao , Wang Mingyang , Zhang Bo , Liu Yue , Zhang Hong , Ma Zhongren , Jin Nengzhi , Lu Huining 
Published: October 2021

Abstract
Hydroxyanthraquinones and anthraquinone glucoside derivatives are always considered as the active antibacterial components. In the present text, a comprehensive comparison and analysis of these compounds were performed for their structure characteristics and antibacterial effect by applying quantum chemical calculations, atoms in molecules theory and molecular docking procedure. The molecular geometric configuration, electrostatic potential, the frontier orbital energies and topological properties were analyzed. Once glucose ring is introduced into the hydroxyanthraquinone rings, almost all of the positive molecular potentials are distributed among the hydroxyl hydrogen atoms of the glucose rings. The anthraquinone glucoside compounds have generally higher intermolecular binding energies than the corresponding aglycones due to the strong interaction between the glucose rings and the surrounding amino acids. Once glucoside ring is introduced into the emodin, low electron density ρ(r) and positive Laplacian value of the O-H bond are the evidences of the highly polarized and covalently decreased bonding interactions. The type of carboxyl, hydroxyl, hydroxylmethyl groups on phenyl ring and the substituent glucose rings are important to the interactions with the topoisomerase type II enzyme DNA gyrase B.
SDVCA – A Robust and Secure Data Communication Protocol for Manet
Authors: Kannan Anusha , N Manikandan , Nandi Mallikarjuna , Chaitanya Morsa , Anilkumar Chunduru 
Published: October 2021

Abstract
The existence of intruders is a threat factor in any network. Degradation of the network operation occur which include delay in transmission, reduced network energy level, low throughput and many. In heterogeneous networks, the threat is complex since it has several interconnected devices working with different operating systems. To solve the trust dispute in the network a Robust and Secure Data Communication Protocol for MANET termed as Software Defined networking enabled Virtual Certification Authority (SDVCA) is proposed. In MANET, the nodes forward information among them. To control the data flow in a MANET the network has to establish a controller node and forwarder nodes for transmitting or forwarding data. The forwarder node carries out the operation of router. The VCA provides authentication certificate for all the network nodes. Without this authentication, the node will be declared as an untrusted intruder. No data will be transferred to or through them. Hence a trusted network is formed and efficient safe data transfer is achieved. In the proposed scheme, the control logic of MANET is moved from forwarding plane (node) to control plane (node) for improving the network performance. A social network model in MANET is designed to create controller-based architecture. This controller node will function as a host and also as a pathway of connecting different social networks in MANET architecture. The results provide improved performances and the network has better throughput, and lesser end to end delay when compared with the existing techniques.
Identifying Simultaneous Matrix Metalloproteinases/Soluble Epoxide Hydrolase Inhibitors
Authors: El-Sherbeni Ahmed A. , Bhatti Rabia , Isse Fadumo A. , El-Kadi Ayman O.S. 
Published: October 2021

Abstract
Matrix metalloproteinase (MMP) and soluble epoxide hydrolase (sEH) have completely unrelated biological functions, however, their dysregulation produce similar effects on biological systems. Based on the similarity in the reported structural requirements for their inhibition, the current study aimed to identify a simultaneous inhibitor for MMP and sEH. Six compounds were identified as potential simultaneous MMP/sEH inhibitors and tested for their capacity to inhibit MMP and sEH. Inhibition of MMP and sEH activity using their endogenous and exogenous substrates was measured by liquid chromatography/mass spectrometry, spectrophotometry and zymography. Two compounds, CTK8G1143 and ONO-4817, were identified to inhibit both MMP and sEH activity. CTK8G1143 and ONO-4817 inhibited the recombinant human sEH activity by an average of 67.4% and 55.2%, respectively. The IC50 for CTK8G1143 and ONO-4817 to inhibit recombinant human sEH were 5.2 and 3.5 µM, respectively, whereas, their maximal inhibition values were 71.4% and 42.8%, respectively. Also, MMP and sEH activity of human cardiomyocytes were simultaneously inhibited by CTK8G1143 and ONO-4817. Regarding other compounds, they showed either MMP or sEH inhibitory activity but not both. In conclusion, these two simultaneous inhibitors of MMP and sEH could provide a promising intervention for the prevention and control of several diseases, especially cardiovascular diseases.
Development of a Split Fluorescent Protein-Based RNA Live-Cell Imaging System to Visualize mRNA Distribution in Plants
Authors: Huang Nien-Chen , Luo Kai-Ren , Yu Tien-Shin 
Published: October 2021

Abstract
Background: RNA live-cell imaging systems have been used to visualize subcellular mRNA distribution in living cells. The MS2-based RNA imaging system exploits the coat protein (MCP) of MS2 bacteriophage with RNA-binding activity and the RNA hairpin structure MS2 binding sequences (MBS) recognized by MCP to indirectly label mRNAs. Co-expression of fluorescent protein (FP)-fused MCP and target mRNA conjugated with multiple copies of MBS allows for visualizing of mRNA localization by confocal microscopy. To minimize the background fluorescence in cytosol, the nucleus localization sequence was used to sequester the MCP-FP not bound to mRNA in the nucleus. However, strong fluorescence in the nucleus may limit the application of RNA visualization in the nucleus and sometimes may interfere in detecting fluorescence signals in cytosol, especially in cells with low signal-to-noise ratio. Results: By using the split FP-based approach, we eliminated the background fluorescence derived from MCP-FP not bound to mRNAs. MCP and PCP, a coat protein of PP7 bacteriophages with RNA-binding activity, were fused with the N- or C-terminus of split FPs (FPN or FPC). On co-expression of MCP-FPN and PCP-FPC with the target mRNA conjugated with multiple copies of the hairpin structure recognized by MCP and PCP, the interaction of RNA and proteins can bring split FPs together to reconstitute functional FPs that allow for visualizing target mRNAs in living cells. We optimized the combinations of MCP- and PCP-FPs with minimal background fluorescence and applied the imaging system to visualize mRNAs in living plant cells.Conclusions: We established a background-free RNA live-cell imaging system that provides a platform to visualize subcellular mRNA distribution in living plant cells.
Convection-Enhanced Delivery in silico study for personalized brain cancer treatment
Authors: Lambride Chryso , Vavourakis Vasileios , Stylianopoulos Triantafyllos 
Published: October 2021

Abstract
Brain cancer therapy remains a formidable challenge in oncology. Convection-enhanced delivery (CED) is an innovative and promising local drug delivery method for the treatment of brain cancer, overcoming the challenges of the systemic delivery of drugs to the brain. To improve our understanding about CED efficacy and drug transport, we present an in silico methodology for brain cancer CED treatment simulation. To achieve this, a three-dimensional finite element biomechanics formulation is utilized which employs patient-specific brain model representation and is used to predict the drug deposition in CED regimes. The model encompasses nonlinear biomechanics and the transport of drugs in the brain parenchyma. Drug distribution was studied under various patho-physiological conditions of the tumor, in terms of tumor vessel wall pore size and tumor tissue hydraulic conductivity as well as for drugs of various sizes, spanning from small molecules to nanoparticles. Our contribution reports for the first time the impact of the size of the vascular wall pores and that of the therapeutic agent on drug distribution during and after CED. The in silico findings provide useful insights of the spatio-temporal distribution and average drug concentration in the tumor towards an effective treatment of brain cancer.
Screening of aflatoxin M1 in cows’ milk in Gadarif town, Sudan
Authors: Abdalmahmoud Kamal M. A. , Shuiep El Tahir S. , Zubeir Ibtisam E El , Arabi Omer H. M. 
Published: October 2021

Abstract
Background

Milk is a perfect complete perishable food that could be contaminated by any substance throughout the chain of its production. Of these aflatoxins M1 was considered as an important health risk associated with the milk. The objectives of this study are to analyse milk samples for the detection of aflatoxin M1.
Method

Eighty milk samples were collected from different sources randomly; 33 samples from farms, 31 samples from sale points and 16 samples from groceries in Gedarif town. Unisensor kits were used as screening method for detection of aflatoxin M1 in milk.
Results

The occurrence of aflatoxin M1 in raw milk samples were found in 22(27.50%) of the samples. The presence of aflatoxin contamination was high in milk samples collected from sales points (15.0%) followed by farms (11.25%) compared to those obtained from groceries (1.25%).
Conclusion

The presences of aflatoxin M1 in the milk samples might indicate that the cow milk was contaminated with aflatoxins through feed. Hence this study recommended that good hygienic practices should be considered in Gadarif town.
Medical Specialists' Perspectives on the Influence of Electronic Medical Record Use on the Quality of Hospital Care: Semistructured Interview Study
Authors: van Poelgeest Rube , Schrijvers Augustinus , Boonstra Albert , Roes Kit 
Published: October 2021

Background
Numerous publications show that electronic medical records (EMRs) may make an important contribution to increasing the quality of care. There are indications that particularly the medical specialist plays an important role in the use of EMRs in hospitals.

Objective
The aim of this study was to examine how, and by which aspects, the relationship between EMR use and the quality of care in hospitals is influenced according to medical specialists.

Methods
To answer this question, a qualitative study was conducted in the period of August-October 2018. Semistructured interviews of around 90 min were conducted with 11 medical specialists from 11 different Dutch hospitals. For analysis of the answers, we used a previously published taxonomy of factors that can influence the use of EMRs.

Results
The professional experience of the participating medical specialists varied between 5 and 27 years. Using the previously published taxonomy, these medical specialists considered technical barriers the most significant for EMR use. The suboptimal change processes surrounding implementation were also perceived as a major barrier. A final major problem is related to the categories “social” (their relationships with the patients and fellow care providers), “psychological” (based on their personal issues, knowledge, and perceptions), and “time” (the time required to select, implement, and learn how to use EMR systems and subsequently enter data into the system). However, the medical specialists also identified potential technical facilitators, particularly in the assured availability of information to all health care professionals involved in the care of a patient. They see promise in using EMRs for medical decision support to improve the quality of care but consider these possibilities currently lacking.

Conclusions
The 11 medical specialists shared positive experiences with EMR use when comparing it to formerly used paper records. The fact that involved health care professionals can access patient data at any time they need is considered important. However, in practice, potential quality improvement lags as long as decision support cannot be applied because of the lack of a fully coded patient record.
Blood Glucose Monitoring and Sharing Amongst People With Diabetes and Their Facilitators: Cross-sectional Study of Methods and Practices
Authors: Jamal Amr , Tharkar Shabana , Babaier Weam Saleh , Alsomali Shrooq Faisal , Alsulayhim Allulu Saad , Alayuni Monera Abdulkareem , Aldakheel Nada Abdulaziz , Al-Osaimi Safa Sultan , Alshehri Norah , Batais Mohammed 
Published: October 2021

Background
The last two decades have witnessed a burgeoning rise in the prevalence of diabetes globally. It has already reached epidemic proportions in Saudi Arabia, with reported high risk among women. As a result, diabetes monitoring and self-management programs are being highly prioritized for diabetes control and management.

Objective
To investigate measuring and sharing practices of the self-monitoring of blood glucose (SMBG) among patients with type 1 or 2 diabetes using insulin.

Methods
A cross-sectional study was conducted on a sample of 203 patients attending primary care clinics at a tertiary care center. The questionnaire assessed the measuring, recording, and sharing of SMBG practices of patients having diabetes with their physicians. The methods used for recording and sharing were categorized into paper-based and electronic-based. In addition, the determinants of the different methods used and frequency of sharing were analyzed.

Results
The overall monitoring prevalence was 95% (193/203), and 57% (117/203) of participants shared the SMBG results. Among the 193 individuals that performed self-monitoring, 138 (72%) performed daily monitoring, and 147 (76%) recorded their blood sugar levels. Almost 55% (81/147) used paper-based materials like notebooks and paper for recording, while the rest (66/147, 45%) used digital devices like laptops and smartphones. A shift towards the use of digital devices and smart applications was observed in patients below 50 years of age. The digitally recorded blood glucose measurements were being shared thrice more often than the recordings made on paper or in notebooks (OR [odds ratio] 2.8; P=.01). Patients >50 years of age (OR 2.3; P=.02), with lesser formal education, married (OR 4.2; P<.001), with smaller family size (OR 2.6; P=.01), having type 2 diabetes (OR 4.1; P<.001) and any comorbid conditions (OR 2.6; P=.01) were associated with higher odds of using paper-based sharing methods. Only the female gender and type 2 diabetes were associated with increased frequency of sharing, while uncontrolled diabetes, the presence of other comorbidities, and duration of diabetes did not show any influence.

Conclusions
Good monitoring and optimal sharing practices were found. Sharing using electronic devices can be emphasized. Diabetes self-management programs can incorporate the use of digital technology in training sessions. Digital literacy and its applications in health care may enhance SMBG practices resulting in better diabetes control.
Analyzing Citizens’ and Health Care Professionals’ Searches for Smell/Taste Disorders and Coronavirus in Finland During the COVID-19 Pandemic: Infodemiological Approach Using Database Logs
Authors: Mukka Milla , Pesälä Samuli , Hammer Charlotte , Mustonen Pekka , Jormanainen Vesa , Pelttari Hanna , Kaila Minna , Helve Otto 
Published: October 2021

Background
The COVID-19 pandemic has prevailed over a year, and log and register data on coronavirus have been utilized to establish models for detecting the pandemic. However, many sources contain unreliable health information on COVID-19 and its symptoms, and platforms cannot characterize the users performing searches. Prior studies have assessed symptom searches from general search engines (Google/Google Trends). Little is known about how modeling log data on smell/taste disorders and coronavirus from the dedicated internet databases used by citizens and health care professionals (HCPs) could enhance disease surveillance. Our material and method provide a novel approach to analyze web-based information seeking to detect infectious disease outbreaks.

Objective
The aim of this study was (1) to assess whether citizens’ and professionals’ searches for smell/taste disorders and coronavirus relate to epidemiological data on COVID-19 cases, and (2) to test our negative binomial regression modeling (ie, whether the inclusion of the case count could improve the model).

Methods
We collected weekly log data on searches related to COVID-19 (smell/taste disorders, coronavirus) between December 30, 2019, and November 30, 2020 (49 weeks). Two major medical internet databases in Finland were used: Health Library (HL), a free portal aimed at citizens, and Physician’s Database (PD), a database widely used among HCPs. Log data from databases were combined with register data on the numbers of COVID-19 cases reported in the Finnish National Infectious Diseases Register. We used negative binomial regression modeling to assess whether the case numbers could explain some of the dynamics of searches when plotting database logs.

Results
We found that coronavirus searches drastically increased in HL (0 to 744,113) and PD (4 to 5375) prior to the first wave of COVID-19 cases between December 2019 and March 2020. Searches for smell disorders in HL doubled from the end of December 2019 to the end of March 2020 (2148 to 4195), and searches for taste disorders in HL increased from mid-May to the end of November (0 to 1980). Case numbers were significantly associated with smell disorders (P<.001) and taste disorders (P<.001) in HL, and with coronavirus searches (P<.001) in PD. We could not identify any other associations between case numbers and searches in either database.

Conclusions
Novel infodemiological approaches could be used in analyzing database logs. Modeling log data from web-based sources was seen to improve the model only occasionally. However, search behaviors among citizens and professionals could be used as a supplementary source of information for infectious disease surveillance. Further research is needed to apply statistical models to log data of the dedicated medical databases.
Pengembangan Instrumen Penilaian Hasil Belajar Ranah Kognitif Model 4D Pada Materi Sepak Bola Berdasarkan Kurikulum 2013
Authors: Wirayasa I Dewa Gede Putra , Darmayasa I Putu , Satyawan I Made 
Published: October 2021

Penelitian ini bertujuan untuk mengembangkan instrumen penilaian hasil belajar ranah kognitif, guna mengetahui validitas isi dan butir, reliabilitas, daya beda, serta tingkat kesukaran dari kelayakan instrumen yang dikembangkan. Jenis penelitian yang digunakan yaitu pengembangan (developmental research). Pada penelitian ini menggunakan model penelitian 4D. Subyek penelitian ini adalah instrumen penilaian hasil belajar ranah kognitif materi sepak bola berupa kisi-kisi dan tes hasil belajar PJOK. Data dikumpulkan menggunakan metode tes. Data yang diperoleh akan dianalisis validitas, reliabilitas, daya beda, serta tingkat kesukaran masing-masing instrumen. Nilai rata-rata dari pengujian analisis data menunjukkan data yang diuji memiliki validitas yang sangat tingi. Hasil pengujian reliabilitas menunjukkan tingkat reliabilitas yang tinggi. Hasil pengujian daya beda, menunjukkan hasil yang bervariasi tiap kelas yang diuji. Tingkat kesukaran menunjukkan hasil pengujian data yang bervariasi pula dalam tingkat kelasnya. Dari hasil pengujian data yang dilaksanakan pada seluruh kelas X di SMA Negeri 1 Amlapura dapat dinyatakan bahwa pengembangan instrumen hasil belajar ranah kognitif materi sepak bola layak dikembangkan dan digunakan dalam penilaian hasil belajar kepada peserta didik.
PENGEMBANGAN INSTRUMEN HASIL BELAJAR PPKN DAN SIKAP SOSIALSISWA KELAS V SD
Authors: K.A.Y. Kawi  , I.W. Lasmawan  , I.M. Gunamantha  
Published: October 2021

Penelitian ini bertujuan untuk mengembangkan instrumen hasil beljar PPKn dan sikap sosial pada siswa kelas V SD yang valid dan reliabel. Penelitian ini menggunakan rancangan penelitian dan pengembangan dengan model pengembangan 4-D. Model ini terdiri atas empat tahap utama yaitu define, design, develop, dan disseminate. Hasil penelitian ini yaitu, (1) Pada tahap define, telah disusunnya kisi-kisi instrumen hasil belajar PPKn dan kisi-kisi instrumen sikap sosial. (2) Pada tahap design, kisi-kisi tersebut dijabarkan menjadi instrumen hasil belajar PPKn berupa tes pilihan ganda serta instrumen sikap sosial berupa angket yang pada awalnya masing-masing terdiri dari 30 butir soal. (3) Pada tahap develop, telah dilakukan uji validitas isi instrumen hasil belajar PPKn mendapatkan hasil sebanyak 27 butir soal dinyatakan valid. Uji validitas isi instrumen sikap sosial mendapatkan hasil sebanyak 25 butir soal dinyatakan valid. Kemudian uji reliabilitas instrumen hasil belajar PPKn memperoleh hasil r11 0,76 ³ 0,70, maka instrumen hasil belajar PPKn dinyatakan reliable. Uji reliabilitas instrumen sikap sosial memperoleh hasil r11 0,78 ³ 0,70, maka instrumen sikap sosial dinyatakan reliable. Dengan demikian pengembngan instrumen hasil belajar PPKn dan sikap sosial dalam penelitian ini dinyatakan valid dan reliabel serta dapat dijadikan contoh untuk mengukur serta mengembangkan instrumen hasil belajar PPKn dan instrumen sikap sosial siswa kelas V.