bluuebunny/crossref_metadata_2025_split huggingface dataset:
Virtual nutrition consultation: what can we learn from the COVID-19 pandemic?
Authors: Kaufman-Shriqui Vered , Sherf-Dagan Shiri , Boaz Mona , Birk Ruth 
Published: January 2021

AbstractObjective:To investigate the extent, quality and challenges of dietetic counselling during the pandemic.Design:A cross-sectional online thirty-six-item Google Survey. The survey queried demographics and information on usage and perceived telemedicine quality.Setting:The survey was distributed to Israeli Dietetic Association (ATID) mailing list between 31 March and 5 May 2020.Participants:Clinical dietitians, members of ATID, who consented to participated in the survey.Results:Three hundred dietitians (12 % of ATID members; 95 % women; mean age 4·41 (sd 10·2) years) replied to the survey. Most dietitians reported a significant ∼30 % decrease in work hours due to the pandemic. The most prevalent form of alternative nutrition counselling (ANC) was over the phone (72 %); 53·5 % used online platforms. Nearly 45 % had no former ANC experience. Both ANC formats were reported inferior to face-to-face nutritional consultation (consultation quality median scores 8 and 7, on a 1–10 scale, for online and phone, respectively). ANC difficulties on either phone or online platforms were technical (56 and 47 %, respectively), lack of anthropometric measurements (28 and 25 %, respectively) and interpersonal communication (19 and 14·6 %, respectively). Older age and former phone counselling experience were associated with higher quality scores, respectively (OR = 1·046, 95 % CI 1·01, 1·08, P = 0·005), (95 % CI 1·38, 4·52, P = 0·02). Those who continued to work full time had five-time greater odds for a higher quality score using online platforms (OR = 5·33, 95 % CI 1·091, 14·89, P = 0·001).Conclusions:Our findings suggest telemedicine holds considerable promise for dietary consultation; however, additional tools and training are needed to optimise remote ANC, especially in light of potential crisis-induced lockdown.
POINTWISE RESIDUAL METHOD FOR SOLVING PRIMAL AND DUAL ILL-POSED LINEAR PROGRAMMING PROBLEMS WITH APPROXIMATE DATA
Authors: IVANITSKIY A. Y. , EJOV V. V. , VASILYEV F. P. 
Published: January 2021

AbstractWe propose a variation of the pointwise residual method for solving primal and dual ill-posed linear programming with approximate data, sensitive to small perturbations. The method leads to an auxiliary problem, which is also a linear programming problem. Theorems of existence and convergence of approximate solutions are established and optimal estimates of approximation of initial problem solutions are achieved.
LECTURES ON THE GEOMETRY AND MODULAR REPRESENTATION THEORY OF ALGEBRAIC GROUPS
Authors: CIAPPARA JOSHUA , WILLIAMSON GEORDIE 
Published: January 2021

AbstractThese notes provide a concise introduction to the representation theory of reductive algebraic groups in positive characteristic, with an emphasis on Lusztig's character formula and geometric representation theory. They are based on the first author's notes from a lecture series delivered by the second author at the Simons Centre for Geometry and Physics in August 2019. We intend them to complement more detailed treatments.
Impact of defaults on participation in state supplemental retirement savings plans
Authors: Clark Robert L. , Pelletier Denis 
Published: January 2021

AbstractThis study examines the impact of the adoption of automatic enrollment provisions by the state of South Dakota for its supplemental retirement saving plan (SRP). In South Dakota, state and local government employees, including teachers, are covered by a defined benefit pension plan and by Social Security. Prior to the introduction of automatic enrollment, the proportion of newly hired employees who were contributing to the SRP was less than 5% in their first year of employment. After the introduction of automatic enrollment, over 90% of newly hired workers who were auto-enrolled were participating in the plan. Using a difference-in-difference approach we find that automatic enrollment changes differences in the participation rate by age, sex, and income. We also find that prior to the adoption of auto-enrollment, agencies that ultimately chose to implement this policy had higher participation rates compared to those that did not adopt auto-enrollment.
Cognitive therapy for moral injury in post-traumatic stress disorder
Authors: Murray Hannah , Ehlers Anke 
Published: January 2021

Abstract
Moral injury is the profound psychological distress that can arise following participating in, or witnessing, events that transgress an individual’s morals and include harming, betraying, or failure to help others, or being subjected to such events, e.g. being betrayed by leaders. It has been primarily researched in the military, but it also found in other professionals such as healthcare workers coping with the COVID-19 pandemic and civilians following a wide range of traumas. In this article, we describe how to use cognitive therapy for post-traumatic stress disorder (CT-PTSD) to treat patients presenting with moral injury-related PTSD. We outline the key techniques involved in CT-PTSD and describe their application to treating patients with moral injury-related PTSD. A case study of a healthcare worker is presented to illustrate the treatment interventions.

Key learning aims

(1)
To recognise moral injury where it arises alongside PTSD.

(2)
To understand how Ehlers and Clark’s cognitive model of PTSD can be applied to moral injury.

(3)
To be able to apply cognitive therapy for PTSD to patients with moral injury-related PTSD.
Pathogen induced subversion of NAD+ metabolism mediating host cell death: a target for development of chemotherapeutics
Authors: Chaurasiya Ayushi , Garg Swati , Khanna Ashish , Narayana Chintam , Dwivedi Ved Prakash , Joshi Nishant , e Anam Zill , Singh Niharika , Singhal Jhalak , Kaushik Shikha , Kaur Kahlon Amandeep , Srivastava Pallavi , Marothia Manisha , Kumar Mukesh , Kumar Santosh , Kumari Geeta , Munjal Akshay , Gupta Sonal , Singh Preeti , Pati Soumya , Das Gobardhan , Sagar Ram , Ranganathan Anand , Singh Shailja 
Published: January 2021

AbstractHijacking of host metabolic status by a pathogen for its regulated dissemination from the host is prerequisite for the propagation of infection. M. tuberculosis secretes an NAD+-glycohydrolase, TNT, to induce host necroptosis by hydrolyzing Nicotinamide adenine dinucleotide (NAD+). Herein, we expressed TNT in macrophages and erythrocytes; the host cells for M. tuberculosis and the malaria parasite respectively, and found that it reduced the NAD+ levels and thereby induced necroptosis and eryptosis resulting in premature dissemination of pathogen. Targeting TNT in M. tuberculosis or induced eryptosis in malaria parasite interferes with pathogen dissemination and reduction in the propagation of infection. Building upon our discovery that inhibition of pathogen-mediated host NAD+ modulation is a way forward for regulation of infection, we synthesized and screened some novel compounds that showed inhibition of NAD+-glycohydrolase activity and pathogen infection in the nanomolar range. Overall this study highlights the fundamental importance of pathogen-mediated modulation of host NAD+ homeostasis for its infection propagation and novel inhibitors as leads for host-targeted therapeutics.
Color-tuning of natural variants of heliorhodopsin
Authors: Kim Se-Hwan , Chuon Kimleng , Cho Shin-Gyu , Choi Ahreum , Meas Seanghun , Cho Hyun-Suk , Jung Kwang-Hwan 
Published: January 2021

AbstractMicrobial rhodopsins are distributed through many microorganisms. Heliorhodopsins are newly discovered but have an unclear function. They have seven transmembrane helices similar to type-I and type-II rhodopsins, but they are different in that the N-terminal region of heliorhodopsin is cytoplasmic. We chose 13 representative heliorhodopsins from various microorganisms, expressed and purified with an N-terminal His tag, and measured the absorption spectra. The 13 natural variants had an absorption maximum (λmax) in the range 530–556 nm similar to proteorhodopsin (λmax = 490–525 nm). We selected several candidate residues that influence rhodopsin color-tuning based on sequence alignment and constructed mutants via site-directed mutagenesis to confirm the spectral changes. We found two important residues located near retinal chromophore that influence λmax. We also predict the 3D structure via homology-modeling of Thermoplasmatales heliorhodopsin. The results indicate that the color-tuning mechanism of type-I rhodopsin can be applied to understand the color-tuning of heliorhodopsin.
Direct visualization of virus removal process in hollow fiber membrane using an optical microscope
Authors: Ayano Miku , Sawamura Yoshiyuki , Hongo-Hirasaki Tomoko , Nishizaka Takayuki 
Published: January 2021

AbstractVirus removal filters developed for the decontamination of small viruses from biotherapeutic products are widely used in basic research and critical step for drug production due to their long-established quality and robust performance. A variety of imaging techniques have been employed to elucidate the mechanism(s) by which viruses are effectively captured by filter membranes, but they are limited to ‘static’ imaging. Here, we propose a novel method for detailed monitoring of ‘dynamic process’ of virus capture; specifically, direct examination of biomolecules during filtration under an ultra-stable optical microscope. Samples were fluorescently labeled and infused into a single hollow fiber membrane comprising cuprammonium regenerated-cellulose (Planova 20N). While proteins were able to pass through the membrane, virus-like particles (VLP) accumulated stably in a defined region of the membrane. After injecting the small amount of sample into the fiber membrane, the real-time process of trapping VLP in the membrane was quantified beyond the diffraction limit. The method presented here serves as a preliminary basis for determining optimum filtration conditions, and provides new insights into the structure of novel fiber membranes.
Multiplex gene and phenotype network to characterize shared genetic pathways of epilepsy and autism
Authors: Peng Jacqueline , Zhou Yunyun , Wang Kai 
Published: January 2021

AbstractIt is well established that epilepsy and autism spectrum disorder (ASD) commonly co-occur; however, the underlying biological mechanisms of the co-occurence from their genetic susceptibility are not well understood. Our aim in this study is to characterize genetic modules of subgroups of epilepsy and autism genes that have similar phenotypic manifestations and biological functions. We first integrate a large number of expert-compiled and well-established epilepsy- and ASD-associated genes in a multiplex network, where one layer is connected through protein–protein interaction (PPI) and the other layer through gene-phenotype associations. We identify two modules in the multiplex network, which are significantly enriched in genes associated with both epilepsy and autism as well as genes highly expressed in brain tissues. We find that the first module, which represents the Gene Ontology category of ion transmembrane transport, is more epilepsy-focused, while the second module, representing synaptic signaling, is more ASD-focused. However, because of their enrichment in common genes and association with both epilepsy and ASD phenotypes, these modules point to genetic etiologies and biological processes shared between specific subtypes of epilepsy and ASD. Finally, we use our analysis to prioritize new candidate genes for epilepsy (i.e. ANK2, CACNA1E, CACNA2D3, GRIA2, DLG4) for further validation. The analytical approaches in our study can be applied to similar studies in the future to investigate the genetic connections between different human diseases.
Synthesis of vinyl ester resin-carrying PVDF green nanofibers for self-healing applications
Authors: Naga Kumar C. , Prabhakar M. N. , Song Jung-il 
Published: January 2021

AbstractSelf-healing on the engineering applications is smart, decisive research for prolonging the life span of the materials and the innovations have been mounting still smarter. Connecting to advancements in self-healing carriers, in altering the chemical structure by optimizing the brittleness for self-healing performance and introducing the bio-degradability, for the first time TPS was blended to PVDF for the synthesis of nanofibers, as carriers of a vinyl ester (VE) resin (medication), by the coaxial electrospinning technique. TPS was mechanically mixed with PVDF base polymer and optimized the TPS content (10 wt%) based on mechanical performance. The novel nanofibers were characterized via field emission scanning electron microscopy (FESEM), Fourier-transform infrared spectroscopy, X-ray diffraction, thermal, moisture analysis, and a mechanical line with FESEM and energy-dispersive X-ray analysis studied the self-healing. The TPS/PVDF fibers having hydrogen bonding and increased the crystallinity (40.57 → 44.12%) and the diameter (115 → 184 nm) along with the surface roughness of the fibers with increasing the TPS content. Microanalysis presented the flow-out of the VE resin at the scratched parts in the pierced fibers; interestingly, after some time, the etched part was cured automatically by the curing of the spread resin. Mechanical stretching of the nanofibers in the tensile tests up in the plastic region showed a decrement in the elasticity (TPS/PVDF fibers) and an increment in the brittle nature (cured VE resin) with the increase in Young’s modulus at each stretching, clearly elucidating the healing performance.
Genomic signatures of the evolution of defence against its natural enemies in the poisonous and medicinal plant Datura stramonium (Solanaceae)
Authors: De-la-Cruz I. M. , Hallab A. , Olivares-Pinto U. , Tapia-López R. , Velázquez-Márquez S. , Piñero D. , Oyama K. , Usadel B. , Núñez-Farfán J. 
Published: January 2021

AbstractTropane alkaloids and terpenoids are widely used in the medicine and pharmaceutic industry and evolved as chemical defenses against herbivores and pathogens in the annual herb Datura stramonium (Solanaceae). Here, we present the first draft genomes of two plants from contrasting environments of D. stramonium. Using these de novo assemblies, along with other previously published genomes from 11 Solanaceae species, we carried out comparative genomic analyses to provide insights on the genome evolution of D. stramonium within the Solanaceae family, and to elucidate adaptive genomic signatures to biotic and abiotic stresses in this plant. We also studied, in detail, the evolution of four genes of D. stramonium—Putrescine N-methyltransferase, Tropinone reductase I, Tropinone reductase II and Hyoscyamine-6S-dioxygenase—involved in the tropane alkaloid biosynthesis. Our analyses revealed that the genomes of D. stramonium show signatures of expansion, physicochemical divergence and/or positive selection on proteins related to the production of tropane alkaloids, terpenoids, and glycoalkaloids as well as on R defensive genes and other important proteins related with biotic and abiotic pressures such as defense against natural enemies and drought.
Gasdermin D restricts Burkholderia cenocepacia infection in vitro and in vivo
Authors: Estfanous Shady , Krause Kathrin , Anne Midhun N. K. , Eltobgy Mostafa , Caution Kyle , Abu Khweek Arwa , Hamilton Kaitlin , Badr Asmaa , Daily Kylene , Carafice Cierra , Baetzhold Daniel , Zhang Xiaoli , Li Tianliang , Wen Haitao , Gavrilin Mikhail A. , Haffez Hesham , Soror Sameh , Amer Amal O. 
Published: January 2021

AbstractBurkholderia cenocepacia (B. cenocepacia) is an opportunistic bacterium; causing severe life threatening systemic infections in immunocompromised individuals including cystic fibrosis patients. The lack of gasdermin D (GSDMD) protects mice against endotoxin lipopolysaccharide (LPS) shock. On the other hand, GSDMD promotes mice survival in response to certain bacterial infections. However, the role of GSDMD during B. cenocepacia infection is not yet determined. Our in vitro study shows that GSDMD restricts B. cenocepacia replication within macrophages independent of its role in cell death through promoting mitochondrial reactive oxygen species (mROS) production. mROS is known to stimulate autophagy, hence, the inhibition of mROS or the absence of GSDMD during B. cenocepacia infections reduces autophagy which plays a critical role in the restriction of the pathogen. GSDMD promotes inflammation in response to B. cenocepacia through mediating the release of inflammasome dependent cytokine (IL-1β) and an independent one (CXCL1) (KC). Additionally, different B. cenocepacia secretory systems (T3SS, T4SS, and T6SS) contribute to inflammasome activation together with bacterial survival within macrophages. In vivo study confirmed the in vitro findings and showed that GSDMD restricts B. cenocepacia infection and dissemination and stimulates autophagy in response to B. cenocepacia. Nevertheless, GSDMD promotes lung inflammation and necrosis in response to B. cenocepacia without altering mice survival. This study describes the double-edged functions of GSDMD in response to B. cenocepacia infection and shows the importance of GSDMD-mediated mROS in restriction of B. cenocepacia.
Low fundamental and formant frequencies predict fighting ability among male mixed martial arts fighters
Authors: Aung Toe , Goetz Stefan , Adams John , McKenna Clint , Hess Catherine , Roytman Stiven , Cheng Joey T. , Zilioli Samuele , Puts David 
Published: January 2021

AbstractHuman voice pitch is highly sexually dimorphic and eminently quantifiable, making it an ideal phenotype for studying the influence of sexual selection. In both traditional and industrial populations, lower pitch in men predicts mating success, reproductive success, and social status and shapes social perceptions, especially those related to physical formidability. Due to practical and ethical constraints however, scant evidence tests the central question of whether male voice pitch and other acoustic measures indicate actual fighting ability in humans. To address this, we examined pitch, pitch variability, and formant position of 475 mixed martial arts (MMA) fighters from an elite fighting league, with each fighter’s acoustic measures assessed from multiple voice recordings extracted from audio or video interviews available online (YouTube, Google Video, podcasts), totaling 1312 voice recording samples. In four regression models each predicting a separate measure of fighting ability (win percentages, number of fights, Elo ratings, and retirement status), no acoustic measure significantly predicted fighting ability above and beyond covariates. However, after fight statistics, fight history, height, weight, and age were used to extract underlying dimensions of fighting ability via factor analysis, pitch and formant position negatively predicted “Fighting Experience” and “Size” factor scores in a multivariate regression model, explaining 3–8% of the variance. Our findings suggest that lower male pitch and formants may be valid cues of some components of fighting ability in men.
On the information hidden in a classifier distribution
Authors: Habibzadeh Farrokh , Habibzadeh Parham , Yadollahie Mahboobeh , Roozbehi Hooman 
Published: January 2021

AbstractClassification tasks are a common challenge to every field of science. To correctly interpret the results provided by a classifier, we need to know the performance indices of the classifier including its sensitivity, specificity, the most appropriate cut-off value (for continuous classifiers), etc. Typically, several studies should be conducted to find all these indices. Herein, we show that they already exist, hidden in the distribution of the variable used to classify, and can readily be harvested. An educated guess about the distribution of the variable used to classify in each class would help us to decompose the frequency distribution of the variable in population into its components—the probability density function of the variable in each class. Based on the harvested parameters, we can then calculate the performance indices of the classifier. As a case study, we applied the technique to the relative frequency distribution of prostate-specific antigen, a biomarker commonly used in medicine for the diagnosis of prostate cancer. We used nonlinear curve fitting to decompose the variable relative frequency distribution into the probability density functions of the non-diseased and diseased people. The functions were then used to determine the performance indices of the classifier. Sensitivity, specificity, the most appropriate cut-off value, and likelihood ratios were calculated. The reference range of the biomarker and the prevalence of prostate cancer for various age groups were also calculated. The indices obtained were in good agreement with the values reported in previous studies. All these were done without being aware of the real health status of the individuals studied. The method is even applicable for conditions with no definite definitions (e.g., hypertension). We believe the method has a wide range of applications in many scientific fields.
Signatures of conserved and unique molecular features in Afrotheria
Authors: Yazhini Arangasamy , Srinivasan Narayanaswamy , Sandhya Sankaran 
Published: January 2021

AbstractAfrotheria is a clade of African-origin species with striking dissimilarities in appearance and habitat. In this study, we compared whole proteome sequences of six Afrotherian species to obtain a broad viewpoint of their underlying molecular make-up, to recognize potentially unique proteomic signatures. We find that 62% of the proteomes studied here, predominantly involved in metabolism, are orthologous, while the number of homologous proteins between individual species is as high as 99.5%. Further, we find that among Afrotheria, L. africana has several orphan proteins with 112 proteins showing < 30% sequence identity with their homologues. Rigorous sequence searches and complementary approaches were employed to annotate 156 uncharacterized protein sequences and 28 species-specific proteins. For 122 proteins we predicted potential functional roles, 43 of which we associated with protein- and nucleic-acid binding roles. Further, we analysed domain content and variations in their combinations within Afrotheria and identified 141 unique functional domain architectures, highlighting proteins with potential for specialized functions. Finally, we discuss the potential relevance of highly represented protein families such as MAGE-B2, olfactory receptor and ribosomal proteins in L. africana and E. edwardii, respectively. Taken together, our study reports the first comparative study of the Afrotherian proteomes and highlights salient molecular features.