bluuebunny/crossref_metadata_2025_split huggingface dataset:
Endothelial cells and coagulation
Authors: Neubauer Katharina , Zieger Barbara 
Published: May 2021

AbstractEndothelial cells form a monolayer, which lines blood vessels. They are crucially involved in maintaining blood fluidity and providing controlled vascular hemostasis at sites of injury. Thereby endothelial cells facilitate multiple mechanisms, including both procoagulant and anticoagulant, which must be kept in balance. Under physiological conditions, endothelial cells constitute a nonadhesive surface preventing activation of platelets and the coagulation cascade. Multiple fibrinolytic and antithrombotic properties act on their cell surface contributing to the maintenance of blood fluidity. These include platelet inhibition, the heparin-antithrombin III system, tissue factor pathway inhibition, thrombomodulin/protein C system, and fibrinolytic qualities. At sites of vascular damage, platelets react immediately by adhering to the exposed extracellular matrix, followed by platelet-platelet interactions to form a clot that effectively seals the injured vessel wall to prevent excessive blood loss. For solid thrombus formation, functional platelets are essential. In this process, endothelial cells serve as a support surface for formation of procoagulant complexes and clotting. This review gives an overview about the central role of the endothelium as a dynamic lining which controls the complex interplay of the coagulation system with the surrounding cells.
Dual temporal gated multi-graph convolution network for taxi demand prediction
Authors: Yang Taoru , Tang Xiaopei , Liu Rong 
Published: May 2021

AbstractTaxi demand prediction is essential to build efficient traffic transportation systems for smart city. It helps to properly allocate vehicles, ease the traffic pressure and improve passengers’ experience. Traditional taxi demand prediction methods mostly rely on time-series forecasting techniques, which cannot model the nonlinearity embedded in data. Recent studies start to combine the Euclidean spatial features through grid-based methods. By considering the spatial correlations among different regions, we can capture how the temporal events have impacts on those with adjacent links or intersections and improve prediction precision. Some graph-based models are proposed to encode the non-Euclidean correlations as well. However, the temporal periodicity of data is often overlooked, and the study units are usually constructed as oversimplified grids. In this paper, we define places with specific semantic and humanistic experiences as study units, using a fuzzy set method based on adaptive kernel density estimation. Then, we introduce dual temporal gated multi-graph convolution network to predict the future taxi demand. Specifically, multi-graph convolution is used to model spatial correlations with graphs, including the neighborhood, functional similarities and landscape similarities based on street view images. As for the temporal dependencies modeling, we design the dual temporal gated branches to capture information hidden in both previous and periodic observations. Experiments on two real-world datasets show the effectiveness of our model over the baselines.
Complete genome sequence of the newly discovered temperate Clostridioides difficile bacteriophage phiCDKH01 of the family Siphoviridae
Authors: Hinc Krzysztof , Kabała Monika , Iwanicki Adam , Martirosian Gajane , Negri Alessandro , Obuchowski Michał 
Published: May 2021

AbstractA temperate siphovirus, phiCDKH01, was obtained from a clinical isolate of Clostridioides difficile. The phage genome is a 45,089-bp linear double-stranded DNA molecule with an average G+C content of 28.7%. It shows low similarity to known phage genomes, except for phiCD24-1. Genomic and phylogenetic analysis revealed that phiCDKH01 is a newly discovered phage. Sixty-six putative ORFs were predicted in the genome, 37 of which code for proteins with predicted functions. The phiCDKH01 prophage was localized in the host genome. The results of this study increase our knowledge about the genetic diversity of tailed phages.
A critical assessment of the stoichiometric knife-edge: no evidence for artifacts caused by the experimental P-supplementation of algae
Authors: Zhou Libin , Declerck Steven A. J. 
Published: May 2021

AbstractThe stoichiometric knife-edge refers to the reduced performance of consumers encountering food with excess phosphorus (P) relative to carbon (C) or nitrogen (N). Studies that provide evidence for such knife-edge in aquatic systems often apply phosphate supplementation to create P-rich food treatments. However, this method may suffer from artifacts, because after uptake algae may store P in a form different from the P-rich biomolecules typically consumed by zooplankton. Our aim was to test if P supplementation results in potential biases. We experimentally exposed populations of the herbivore rotifer species, Brachionus calyciflorus (Pallas), to four different food quality treatments: algae grown under P-saturating (HPchem, molar C:P ratio = 59.7 ± 2.7) and P-sufficient (MPchem, molar C:P = 116.3 ± 5.2) conditions in chemostats, and algae grown under P-limiting conditions, but with molar C:P ratios equal to HPchem and MPchem treatments, respectively (HPLP+P, molar C:P = 59.8 ± 0.14; MPLP+P, molar C:P = 121.0 ± 4.3). The latter two treatments were achieved through P-supplementation of P-limited algae. Results show that for rotifers fed algae with either excess or intermediate P content, population growth rates were consistently higher on algae grown in chemostats than algae treated with the P supplementation method. Importantly, growth rates were also consistently lower in HP than in MP treatments and the magnitude of this negative impact was independent on algal growth history. The latter result confirms the existence of a stoichiometric knife-edge and indicates that P supplementation is a reliable method to study the relative effect of excess P on zooplankton performance in a standardized way.
Provision of astigmatid mites as supplementary food increases the density of the predatory mite Amblyseius swirskii in greenhouse crops, but does not support the omnivorous pest, western flower thrips
Authors: Pirayeshfar Fatemeh , Safavi Seyed Ali , Moayeri Hamid Reza Sarraf , Messelink Gerben J. 
Published: May 2021

AbstractAstigmatid mites can be used as prey for mass rearing of phytoseiid predators, but also as a supplemental food source to support predator populations in crops. Here we evaluated the potential of six species of astigmatid mites (living or frozen) as alternative food for the predatory mite Amblyseius swirskii Athias-Henriot in greenhouse crops. All prey mites tested were suitable for predator oviposition. In general, oviposition was greater when prey mites were reared on dog food with yeast than when they were reared on wheat bran with yeast. Amongst prey items provided as frozen diet, larvae of Thyreophagus entomophagus (Laboulbene), Acarus siro L. and Lepidoglyphus destructor (Schrank) that had been reared on dog food with yeast, resulted in the highest oviposition rates of A. swirskii. T. entomophagus larvae as frozen diet resulted in the shortest preimaginal developmental time of A. swirskii. On chrysanthemum plants, we found that the greatest increase in predator density occurred when living mites of T. entomophagous were used as a food source. This increase was greater than when predators were fed cattail pollen, a commonly used supplemental food. Effects on predators of providing living A. siro and L. destructor, or frozen larvae of T. entomophagous as food, were comparable with provision of pollen. Use of supplemental food in crops can be a risk if it is also consumed by omnivorous pests such as western flower thrips, Frankliniella occidentalis Pergande. However, we showed that both frozen and living mites of T. entomophagous were unsuitable for thrips oviposition. Hence, we believe that provision of prey mite species increases A. swirskii density, supporting biological control of thrips and other pests in greenhouse crops.
The Manifold Of Variations: impact location of short-term impactors
Authors: Del Vigna Alessio , Dimare Linda , Bracali Cioci Davide 
Published: May 2021

AbstractThe interest in the problem of small asteroids observed shortly before a deep close approach or an impact with the Earth has grown a lot in recent years. Since the observational dataset of such objects is very limited, they deserve dedicated orbit determination and hazard assessment methods. The currently available systems are based on the systematic ranging, a technique providing a two-dimensional manifold of orbits compatible with the observations, the so-called Manifold Of Variations. In this paper we first review the Manifold Of Variations method, to then show how this set of virtual asteroids can be used to predict the impact location of short-term impactors, and compare the results with those of already existent methods.
Divergent Dimethylarginine Dimethylaminohydrolase Isoenzyme Expression in the Central Nervous System
Authors: Kozlova Alena A. , Ragavan Vinitha N. , Jarzebska Natalia , Lukianova Iana V. , Bikmurzina Anastasia E. , Rubets Elena , Suzuki-Yamamoto Toshiko , Kimoto Masumi , Mangoni Arduino A. , Gainetdinov Raul R. , Weiss Norbert , Bauer Michael , Markov Alexander G. , Rodionov Roman N. , Bernhardt Nadine 
Published: May 2021

AbstractThe endogenous methylated derivative of ʟ-arginine, Nω,Nω′-dimethyl-ʟ-arginine (asymmetric dimethylarginine, ADMA), an independent risk factor in many diseases, inhibits the activity of nitric oxide synthases and, consequently, modulates the availability of nitric oxide. While most studies on the biological role of ADMA have focused on endothelial and inducible nitric oxide synthases modulation and its contribution to cardiovascular, metabolic, and renal diseases, a role in regulating neuronal nitric oxide synthases and pathologies of the central nervous system is less understood. The two isoforms of dimethylarginine dimethylaminohydrolase (DDAH), DDAH1 and DDAH2, are thought to be the main enzymes responsible for ADMA catabolism. A current impediment is limited knowledge on specific tissue and cellular distribution of DDAH enzymes within the brain. In this study, we provide a detailed characterization of the regional and cellular distribution of DDAH1 and DDAH2 proteins in the adult murine and human brain. Immunohistochemical analysis showed a wide distribution of DDAH1, mapping to multiple cell types, while DDAH2 was detected in a limited number of brain regions and exclusively in neurons. Our results provide key information for the investigation of the pathophysiological roles of the ADMA/DDAH system in neuropsychiatric diseases and pave the way for the development of novel selective therapeutic approaches.
The role of reading comprehension in mathematical modelling: improving the construction of a real-world model and interest in Germany and Taiwan
Authors: Krawitz Janina , Chang Yu-Ping , Yang Kai-Lin , Schukajlow Stanislaw 
Published: May 2021

AbstractTo solve mathematical modelling problems, students must translate real-world situations, which are typically presented in text form, into mathematical models. To complete the translation process, the problem-solver must first understand the real-world situation. Therefore, reading comprehension can be considered an essential part of solving modelling problems, and fostering reading comprehension might lead to better modelling competence. Further, ease of comprehension and involvement have been found to increase interest in the learning material, and thus, improving reading comprehension might also increase interest in modelling. The aims of this study were to (a) determine whether providing students with reading comprehension prompts would improve the modelling sub-competencies needed to construct a model of the real-world situation and their interest in modelling and (b) analyze the hypothesized effects in two different educational environments (Germany and Taiwan). We conducted an experimental study of 495 ninth graders (201 German and 294 Taiwanese students). The results unexpectedly revealed that providing reading comprehension prompts did not affect the construction of a real-world model. Further, providing reading comprehension prompts improved students’ situational interest. The effects of providing reading comprehension prompts on the construction of a real-world model were similar in Germany and Taiwan. Students’ interest in modelling improved more in Germany. An in-depth quantitative analysis of students’ responses to reading prompts, their solutions, and their interest in the experimental group confirmed the positive relation between reading comprehension and modelling and indicated that the reading comprehension prompts were not sufficient for improving reading comprehension. Implications for future research are discussed.
A thermoeconomic indicator for the sustainable development with social considerations
Authors: Lucia Umberto , Fino Debora , Grisolia Giulia 
Published: May 2021

AbstractThe United Nations action plan Agenda 21 has represented a milestone toward Sustainable Development. On its 40th Chapter, it is introduced the requirement to dispose of an accurate and continuous collection of information, essential for decision-making. Besides bridging the data gap and improving the information availability, it is highlighted the need to dispose of sustainable development indicators, in order to assess and monitor the performances of countries toward sustainability. In this paper, we develop an improvement of a new indicator, recently introduced linking environmental anthropic footprint and social and industrial targets. Here, we suggest a link with the Income Index, in order to consider also a condition of people well-being. Our results consists in an improvement of the present approaches to sustainability; indeed, we link the socio-economic considerations, quantified by the Income Index and the Human Development Index, to the engineering approach to optimization, introducing the thermodynamic quantity entropy generation, related to irreversibility. In this way, two different new indicators are introduced, the Thermodynamic Income Index and the Thermodynamic Human Development Index, which quantitatively express a new viewpoint, which goes beyond the dichotomy between socio-economic considerations on one hand and engineering and scientific approach to sustainability on the other one. So, the result leads to a unified tool useful for the designing of new policies and interventions for a sustainable development for the next generations.
Stability of Discontinuous Galerkin Spectral Element Schemes for Wave Propagation when the Coefficient Matrices have Jumps
Authors: Kopriva David A. , Gassner Gregor J. , Nordström Jan 
Published: May 2021

AbstractWe use the behavior of the$$L_{2}$$L2norm of the solutions of linear hyperbolic equations with discontinuous coefficient matrices as a surrogate to infer stability of discontinuous Galerkin spectral element methods (DGSEM). Although the$$L_{2}$$L2norm is not bounded in terms of the initial data for homogeneous and dissipative boundary conditions for such systems, the$$L_{2}$$L2norm is easier to work with than a norm that discounts growth due to the discontinuities. We show that the DGSEM with an upwind numerical flux that satisfies the Rankine–Hugoniot (or conservation) condition has the same energy bound as the partial differential equation does in the$$L_{2}$$L2norm, plus an added dissipation that depends on how much the approximate solution fails to satisfy the Rankine–Hugoniot jump.
When Manual Analysis of 12-Lead ECG Holter Plays a Critical Role in Discovering Unknown Patterns of Increased Arrhythmogenic Risk: A Case Report of a Patient Treated with Tamoxifen and Subsequent Pneumonia in COVID-19
Authors: Brisinda Donatella , Merico Barbara , Fenici Peter , Fenici Riccardo 
Published: May 2021

AbstractSeveral medicines, including cancer therapies, are known to alter the electrophysiological function of ventricular myocytes resulting in abnormal prolongation and dispersion of ventricular repolarization (quantified by multi-lead QTc measurement). This effect could be amplified by other concomitant factors (e.g., combination with other drugs affecting the QT, and/or electrolyte abnormalities, such as especially hypokalemia, hypomagnesaemia, and hypocalcemia). Usually, this condition results in higher risk of torsade de point and other life-threatening arrhythmias, related to unrecognized unpaired cardiac ventricular repolarization reserve (VRR). Being VRR a dynamic phenomenon, QT prolongation might often not be identified during the 10-s standard 12-lead ECG recording at rest, leaving the patient at increased risk for life-threatening event. We report the case of a 49-year woman, undergoing tamoxifen therapy for breast cancer, which alteration of ventricular repolarization reserve, persisting also after correction of concomitant recurrent hypokalemia, was evidenced only after manual measurements of the corrected QT (QTc) interval from selected intervals of the 12-lead ECG Holter monitoring. This otherwise missed finding was fundamental to drive the discontinuation of tamoxifen, shifting to another “safer” therapeutic option, and to avoid the use of potentially arrhythmogenic antibiotics when treating a bilateral pneumonia in recent COVID-19.
Gene Expression Changes in a Model Neuron Cell Line Exposed to Autoantibodies from Patients with Traumatic Brain Injury and/or Type 2 Diabetes
Authors: Zimering Mark B. , Delic Vedad , Citron Bruce A. 
Published: May 2021

AbstractTraumatic brain injury and adult type 2 diabetes mellitus are each associated with the late occurrence of accelerated cognitive decline and Parkinson’s disease through unknown mechanisms. Previously, we reported increased circulating agonist autoantibodies targeting the 5-hydroxytryptamine 2A receptor in plasma from subsets of Parkinson’s disease, dementia, and diabetic patients suffering with microvascular complications. Here, we use a model neuron, mouse neuroblastoma (N2A) cell line, to test messenger RNA expression changes following brief exposure to traumatic brain injury and/or type 2 diabetes mellitus plasma harboring agonist 5-hydroxytryptamine 2A receptor autoantibodies. We now report involvement of the mitochondrial dysfunction pathway and Parkinson’s disease pathways in autoantibody-induced gene expression changes occurring in neuroblastoma cells. Functional gene categories upregulated significantly included cell death, cytoskeleton-microtubule function, actin polymerization or depolymerization, regulation of cell oxidative stress, mitochondrial function, immune function, protein metabolism, and vesicle function. Gene categories significantly downregulated included microtubule function, cell adhesion, neurotransmitter release, dopamine metabolism synaptic plasticity, maintenance of neuronal differentiation, mitochondrial function, and cell signaling. Taken together, these results suggest that agonist 5-hydroxytryptamine receptor autoantibodies (which increase in Parkinson’s disease and other forms of neurodegeneration) mediate a coordinating program of gene expression changes in a model neuron which predispose to neuro-apoptosis and are linked to human neurodegenerative diseases pathways.
Driving investments in ore beneficiation and scrap upgrading to meet an increased demand from the direct reduction-EAF route
Authors: Gyllenram Rutger , Arzpeyma Niloofar , Wei Wenjing , Jönsson Pär G. 
Published: May 2021

AbstractThe pressure on the steel industry to reduce its carbon footprint has led to discussions to replace coke as the main reductant for iron ore and turn to natural gas, bio-syngas or hydrogen. Such a major transition from the blast furnace-basic oxygen furnace route, to the direct reduction-electric arc furnace route, for steel production would drastically increase the demand for both suitable iron ore pellets and high-quality scrap. The value for an EAF plant to reduce the SiO2 content in DRI by 2 percentage points and the dirt content of scrap by 0.3 percentage points Si was estimated by using the optimization and calculation tool RAWMATMIX®. Three plant types were studied: (i) an integrated plant using internal scrap, (ii) a plant using equal amounts of scrap and DRI and (iii) a plant using a smaller fraction of DRI in relation to the scrap amount. Also, the slag volume for each plant type was studied. Finally, the cost for upgrading was estimated based on using mainly heuristic values. A conservative estimation of the benefit of decreasing the silica content in DRI from 4 to 2% is 20 USD/t DRI or 15 USD/t DR pellets and a conservative figure for the benefit of decreasing the dirt in scrap by 0.3 percentage points Si is 9 USD/t scrap. An estimate on the costs for the necessary ore beneficiation is 2.5 USD/t pellet concentrate and for a scrap upgrade, it is 1-2 USD/t scrap.
An evidence-based overview of hybridization in tinamous
Authors: Ottenburghs Jente 
Published: May 2021

AbstractEstimates suggest that about 16% of bird species hybridize in the wild. This number is based on two main sources: the Handbook of Avian Hybrids of the World by Eugene McCarthy and the online Serge Dumont Bird Hybrids Database. Although both sources provide supporting references for the documented hybrids, the reliability of these references has not been systematically assessed. In this paper, I introduce a scoring scheme based on three criteria that are weighted based on their reliability, namely field observations or photographs (1 point), morphological analyses (2 points), and genetic analyses (3 points). The final tally of these three criteria (ranging from 0 to 6 points) will indicate the level of confidence for a particular hybrid. I test this scoring scheme on the Neotropical bird family Tinamidae (tinamous), in which several putative hybrids have been reported. My analysis revealed one well-documented case (Crypturellus boucardi×C. cinnamomeus) and three doubtful records that require further investigation. These findings highlight the need for thoroughly scrutinizing the sources supporting avian hybrids. The scoring system clearly illustrates its usefulness and can be easily applied to other taxonomic groups to increase the reliability of documenting interspecific hybrids.