bluuebunny/crossref_metadata_2025_split huggingface dataset:
Ly-6A-Induced Growth Inhibition and Cell death in a Transformed CD4+T cell line: Role of Tumor Necrosis Factor-α (TNF-α)
Authors: Patel Akshay G. , Moxham Sarah , Bamezai Anil K. 
Published: November 2021

AbstractEngaging the Ly-6A protein, an inhibitory signaling protein, on CD4+T cell lines triggers apoptosis. Signaling through Ly-6A activates cell-intrinsic apoptotic cell death pathway as indicated by release of cytochrome C, activation of caspase 3 and 9. In addition Ly-6A induces cytokine production and growth inhibition. The mechanism underlying simultaneous distinct cellular responses has remained unknown. To examine the relatedness of distinct responses generated by engaging Ly-6A, we have quantified the secretion of TNFα, TGFβ and a related protein GDF10, the three pro-apoptotic, growth inhibitory and tumor suppressive cytokines. While low levels of TGFβ and GDF10 were detected after engaging Ly-6A, the production of TNF-α was elevated in cell cultures stimulated the Ly-6A protein. Blocking the biological activity of TNFα resulted in reduced apoptosis induced by engaging Ly-6A. In contrast, growth inhibition/apoptosis in response to antigen receptor complex stimulation was not observed. Engaging the antigen receptor through activating the epsilon (ε) chain of CD3 generated high levels of TGF-β and GDF10 while decreasing TNFα. These results suggest that the TNF-α cytokine contributes to the Ly-6A-induced growth inhibitory and pro-apoptotic response in CD4+T cells and provides mechanistic explanation of the observed biologically distinct responses initiated after engaging Ly-6A protein. These findings aid in understanding the inhibitory signaling initiated by Ly-6A protein, especially in the context of its potential immune checkpoint inhibitory role in T cells.
Birnaviridae virus factories show features of liquid-liquid phase separation, and are distinct from paracrystalline arrays of virions observed by electron microscopy
Authors: Reddy Vishwanatha R. A. P. , Campbell Elle A. , Wells Joanna , Simpson Jennifer , Nazki Salik , Hawes Philippa C. , Broadbent Andrew J. 
Published: November 2021

AbstractTo gain more information about the nature of Birnaviridae virus factories (VFs), we used a recombinant infectious bursal disease virus (IBDV) expressing split-GFP11 tagged to the polymerase (VP1) that we have previously shown is a marker for VFs in infected cells expressing GFP1-10. We found that VFs co-localized with 5-ethynyl uridine in the presence of actinomycin D, confirming they were the site of de novo RNA synthesis, and VFs were visible in infected cells that were fixed and permeabilized with digitonin, demonstrating that they were not membrane bound. Fluorescence recovery after photobleaching (FRAP) a region of interest within the VFs occurred rapidly, recovering from approximately 25% to 87% the original intensity over 146 seconds, and VFs were dissolved by 1,6-hexanediol treatment, demonstrating they showed properties consistent with liquid-liquid phase separation. There was a lower co-localization of the VF GFP signal with the capsid protein VP2 (Manders’ coefficient (MC) 0.6), compared to VP3 (MC, 0.9), which prompted us to investigate the VF ultrastructure by transmission electron microscopy (TEM). In infected cells, paracrystalline arrays (PAs) of virions were observed in the cytoplasm, as well as discrete electron dense regions. Using correlative light and electron microscopy (CLEM), we observed that the electron dense regions correlated with the GFP signal of the VFs, which were distinct from the PAs. In summary, Birnaviridae VFs are sites of de novo RNA synthesis, are not bound by a membrane, show properties consistent with liquid-liquid phase separation, and are distinct from the PAs observed by TEM.ImportanceMembers of the Birnaviridae infect birds, fish and insects, and are responsible for diseases of significant economic importance to the poultry industry and aquaculture. Despite their importance, how they replicate in cells remains poorly understood. Here, we show that the Birnaviridae virus factories are not membrane bound, demonstrate properties consistent with liquid-liquid phase separation, and are distinct from the paracrystalline arrays of virions observed by transmission electron microscopy, enhancing our fundamental knowledge of virus replication that could be used to develop strategies to control disease, or optimize their therapeutic application.
COVID-19 first and delta waves in relation to ACEI, ARB, Influenza vaccination, and comorbidity in a North Metropolitan Barcelona Health Consortium
Authors: Juanes-González Marta , Calderón-Valdiviezo Ana , Losa-Puig Helena , Valls-Foix Roger , González-Salvador Marta , León-Pérez Marc , Pueyo-Antón Luís , Lozano-Paz Celia , Franco-Romero Maite , Vidal-Alaball Josep , Puigdellívol-Sánchez Anna 
Published: November 2021

ABSTRACTBACKGROUNDSome authors have reported that angiotensin converter enzyme inhibitors (ACEI) and angiotensin receptor blockers (ARB) improve clinical outcomes in hypertensive COVID-19 patients, and others have proposed cross-protection for influenza vaccination. This study explores the impact of these variables on the evolution of hospitalized patients, focusing in the first wave and the Delta wave.METHODSHospitalizations (n=1888) from March 1, 2020, to July 31, 2021, in the Hospital of Terrassa, the referral center for the free access Terrassa Health Consortium in the North Metropolitan Barcelona Health Region (population=167,386) were studied. The number of chronic treatments and conditions of patients from the initial outbreak (n=184) and the Delta outbreak (n=158) were recorded.RESULTSOf the non-survivors, 96.3% were aged >60 years in the first wave and 100% were aged >70 years in the Delta wave. In non-survival hospitalized patients aged >60 years, the percentage treated with ACEI was similar to general population but was significantly different for ARB treatments of influenza vaccination, although associated to a higher comorbidity and age. In July 2021, the number of hospitalizations for patients aged <50 years was higher than March 2020 and 22% of hospitalized patients without chronic treatments and conditions needed admission to the intensive care unit. Mortality was reduced in the groups with most comorbidities who received influenza and SARS-CoV2 vaccination.CONCLUSIONSIn COVID-19 infection, age and comorbidity are related to survival, ACEI use is safe. A high proportion of patients without comorbidity require hospitalization and intensive care.
Frequency and phenotype associations of rare variants in five monogenic cerebral small vessel disease genes in 200,000 UK Biobank participants with whole exome sequencing data
Authors: Ferguson Amy C. , Thrippleton Sophie , Henshall David E. , Whittaker Ed , Conway Bryan , MacLeod Malcolm , Malik Rainer , Rawlik Konrad , Tenesa Albert , Sudlow Cathie , Rannikmäe Kristiina 
Published: November 2021

AbstractBased on previous case reports and disease-based cohorts, a minority of patients with cerebral small vessel disease (cSVD) have a monogenic cause, with many also manifesting extra-cerebral phenotypes. We investigated the frequency, penetrance, and phenotype associations of rare variants in cSVD genes in UK Biobank (UKB), a large population-based study.We used a systematic review of previous literature and ClinVar to identify putative pathogenic rare variants in CTSA, TREX1, HTRA1, COL4A1/2. We mapped phenotypes previously attributed to these variants (phenotypes-of-interest) to disease coding systems used in UKB’s linked health data from UK hospital admissions, death records and primary care. Among 199,313 exome-sequenced UKB participants, we assessed: the proportion of participants carrying ≥1 variant(s); phenotype-of-interest penetrance; and the association between variant carrier status and phenotypes-of-interest using a binary (any phenotype present/absent) and phenotype burden (linear score of the number of phenotypes a participant possessed) approach.Among UKB participants, 0.5% had ≥1 variant(s) in studied genes. Using hospital admission and death records, 4-20% of variant carriers per gene had an associated phenotype. This increased to 7-55% when including primary care records. Only COL4A1 variant carrier-status was significantly associated with having ≥1 phenotype-of-interest and a higher phenotype score (OR=1.29, p=0.006).While putative pathogenic rare variants in monogenic cSVD genes occur in 1:200 people in the UKB population, only around half of variant carriers have a relevant disease phenotype recorded in their linked health data. We could not replicate most previously reported gene-phenotype associations, suggesting lower penetrance rates, overestimated pathogenicity and/or limited statistical power.
Business process nature assessment matrix – a novel approach to the assessment of business process nature
Authors: Berniak-Woźny Justyna , Szelągowski Marek 
Published: November 2021

PurposeThe purpose of this study is to give an in-depth understanding of the nature of business processes (BPs) from the perspective of their dynamism and knowledge intensity that will allow for their correct classification and provide practical and useful implications for their more relevant and effective management. A simple and low labor-intensive BP nature assessment approach is proposed that will allow for objective assessment and internal benchmarking of all BPs in a specific context of execution based on their nature.Design/methodology/approachThe research is divided into two parts. The first comprises a systematic literature review (SLR) based on the resources of the ProQuest, Springer Nature and ScienceDirect full-text databases and the second includes illustrative case studies.FindingsBuilding on the SLR, the authors identified and reviewed 3,385 articles and defined a set of criteria by which the nature of BPs can be assessed. Further, the authors proposed a BP nature assessment matrix together with complementary questionnaires for the evaluation of process dynamism and knowledge intensity. To demonstrate the logic of this approach, two illustrative case studies were presented.Originality/valueThe article contributes to the theoretical reflection on the nature of BPs in the knowledge economy. From the practical point of view, a novel approach to the assessment of the nature of BPs is offered. The approach is open and as experience is accumulated it will develop according to the data and recommendations collected.
Principals' intentions and anticipated challenges in implementing nutrition education
Authors: Baloch Fozia Ahmed , Mohamed Ismail Shaik Abdul Malik , Jogezai Nazir Ahmed 
Published: November 2021

PurposeThis study aimed to know principals' intentions of implementing nutrition education (NE) and explore the challenges they may anticipate while implementing NE in their respective schools.Design/methodology/approachThe study employed a sequential mixed-method design using a questionnaire and interviews to collect data. The quantitative data were collected from 378 secondary school principals, while 16 school principals were interviewed.FindingsThe study found principals with positive intentions towards implementing NE. The results also highlighted challenges related to capacity building, resources (both human and physical), policies and plans, roles, and responsibilities of the implementers (teachers and principals), support from higher authorities, community participation and teachers' unions. This study concludes that principals' intentions remain central to the implementation of NE in schools. However, it is necessary to overcome those challenges before its implementation.Research limitations/implicationsThe study sought to ascertain principals' intentions rather than their actual behaviour of NE implementation and hence remains limited in this area, which future research may consider. Furthermore, the research is limited to the principals' opinions on the anticipated challenges associated with NE implementation. The study did not solicit the opinions of other stakeholders, such as education managers, policymakers, teachers and communities.Originality/valueThe article is significant in terms of NE being implemented in schools to improve students' physical and academic wellbeing. The critical role of principals has been investigated by determining their intentions and the anticipated challenges associated with implementing NE. The authors declare the originality of the data.
An optimal scheduling policy for upgraded software with updates
Authors: Anand Adarsh , Das Subhrata , Agarwal Mohini , Inoue Shinji 
Published: November 2021

PurposeIn the current market scenario, software upgrades and updates have proved to be very handy in improving the reliability of the software in its operational phase. Software upgrades help in reinventing working software through major changes, like functionality addition, feature enhancement, structural changes, etc. In software updates, minor changes are undertaken which help in improving software performance by fixing bugs and security issues in the current version of the software. Through the current proposal, the authors wish to highlight the economic benefits of the combined use of upgrade and update service. A cost analysis model has been proposed for the same.Design/methodology/approachThe article discusses a cost analysis model highlighting the distinction between launch time and time to end the testing process. The number of bugs which have to be catered in each release has been determined which also consists of the count of latent bugs of previous version. Convolution theory has been utilized to incorporate the joint role of tester and user in bug detection into the model. The cost incurred in debugging process was determined. An optimization model was designed which considers the reliability and budget constraints while minimizing the total debugging cost. This optimization was used to determine the release time and testing stop time.FindingsThe proposal is backed by real-life software bug dataset consisting of four releases. The model was able to successfully determine the ideal software release time and the testing stop time. An increased profit is generated by releasing the software earlier and continues testing long after its release.Originality/valueThe work contributes positively to the field by providing an effective optimization model, which was able to determine the economic benefit of the combined use of upgrade and update service. The model can be used by management to determine their timelines and cost that will be incurred depending on their product and available resources.
Quality 4.0 – a measurement model using the confirmatory factor analysis (CFA) approach
Authors: Sureshchandar G.S. 
Published: November 2021

PurposeThe disruptions caused by new-age technologies of Industry 4.0 are posing a formidable challenge to researchers, academicians and practitioners alike. Quality 4.0 that depicts the role of the quality function in the Industry 4.0 scenario must be comprehended so that the rudiments of Quality 4.0 are understood properly, and interventions can be made to embrace the new normal. As the literature on Quality 4.0 is extremely scarce, empirical studies are mandatory to augment the process of theory building.Design/methodology/approachThe research work identifies 12 axes of the Quality 4.0 revolution based on literature review and insights from experts. Subsequently, a measurement model is formulated and an instrument to measure the level of Quality 4.0 implementation is developed. The measurement model has been checked for model fit, reliability and validity using the confirmatory factor analysis approach.FindingsThe proposed model was found to be adequate, reliable and valid and concludes that though technology plays a significant role in the development of the Quality 4.0 system, aspects of traditional quality are very much apropos to transform to the next frontier of quality.Research limitations/implicationsImplications for future research are provided which would help to further explore the nascent field of Quality 4.0.Practical implicationsThis research would help the practitioners better understand the various requirements and measure the degree of implementation of a Quality 4.0 system.Originality/valueThe present research is perhaps the first of its kind in propounding a measurement model, through empirical analysis, for the betterment of the understanding of Quality 4.0 and its associated constituents.
Dynamic contact stress and frictional heat analysis of femoral head-on-acetabular cup interface based on calculation and simulation methods
Authors: Fang Xingxing , Li Dahan , Xin Yucheng , Wang Songquan , Guo Yongbo , Hu Ningning , Zhang Dekun 
Published: November 2021

Purpose
The purpose of this paper is to systematically study the dynamic contact stress, frictional heat and temperature field of femoral head-on-acetabular cup contact pairs in a gait cycle.

Design/methodology/approach
In this paper, four common femoral head-on-acetabular cup contact pairs are used as the research objects, mathematical calculations and finite element simulations are adopted. The contact model of hip joint head and acetabular cup was established by finite element simulation to analyze the stress and temperature distribution of the contact interface.

Findings
The results show that the contact stress of the head-on-cup interface is inversely proportional to the contact area; high contact stress directly leads to greater frictional heat. However, hip joints with metal-on-polyethylene or ceramic-on-polyethylene paired interfaces have lower frictional heat and show a significant temperature rise in one gait cycle, which may be related to the material properties of the acetabular cup.

Originality/value
Previous studies about calculating the interface frictional heat always ignore the dynamic change process in the contact load and the contact area. This study considered the dynamic changes of the contact stress and area of the femoral head-on-acetabular cup interface, and four common contact pairs were systematically analyzed.
Effect of achievement-related gamification on brand attachment
Authors: Yang Congcong , Yang Pianpian , Feng Yuanyue 
Published: November 2021

PurposeThis research draws on self-determination theory and self-construal theory to investigate the effects of achievement-related gamification features on customers' brand attachment in online brand communities.Design/methodology/approachSurvey data (N = 523) were collected from customers of typical gamified online brand communities. The research model was examined with structural equation modeling using SmartPLS 3.2.FindingsAchievement-related gamification features positively affect customers' brand attachment via the intrinsic motives of autonomy, competence and relatedness, but not extrinsic motives. Relatedness motive exerts the strongest mediating effect between achievement-related gamification features and brand attachment, followed by competence and autonomy. Moreover, interdependent self-construal positively moderates the effects of achievement-related gamification features on intrinsic (autonomy, competence and relatedness) and extrinsic motives.Originality/valueTheoretically, this study contributes to the brand marketing literature by identifying gamification as a new antecedent for brand attachment. It also contributes to the gamification literature by demonstrating that achievement-related gamification features strengthen brand attachment via intrinsic motives but not extrinsic motives in the specific context of the online brand community. Meanwhile, the relatedness motive exerts the strongest mediating effect between achievement-related gamification features and brand attachment, followed by competence and autonomy. Moreover, this research provides the first empirical evidence that interdependent self-construal moderates the effects of achievement-related gamification features on intrinsic and extrinsic motives. Practically, managers can learn from the results that achievement-related gamification features should be designed to foster customers' intrinsic motives and brand attachment, especially those high in interdependent self-construal levels.
Navigating the US “Green Rush”: anti-money laundering and de-risking implications for banking cannabis-related businesses in Jamaica
Authors: Clarke Rohan 
Published: November 2021

Purpose
This paper aims to illuminate the diverging approaches to marijuana-related drug enforcement at the federal and state levels in the USA, which have facilitated a boom in the US medical cannabis industry (i.e. the “Green Rush”). It further sheds light on how the USA’ aggressive extraterritorial approach to anti-money laundering (AML) enforcement might simultaneously suppress the banking of cannabis-related businesses in Jamaica due to the lingering fear of de-risking.

Design/methodology/approach
An international and comparative legal and policy analysis was conducted of the nexus among shifting drug enforcement policies, AML laws and the banking of cannabis-related businesses.

Findings
This study found that the constitutional relationship between the US federal government and states has created a de facto comparative advantage for the US medical cannabis-related businesses that benefit from limited access to financial services. This was found to pose far-reaching implications for the banking and development of the Jamaican cannabis sector due to the dependence of the country’s financial institutions on correspondent banking relationships with the US banks that are regulated by federal AML statutes.

Originality/value
To the best of the author’s knowledge, this paper is the first of its kind to examine the extraterritorial regulatory risks to the banking of cannabis-related businesses in Jamaica.
Counterproductive knowledge behavior in volunteer work: perspectives from the theory of planned behavior and well-being theory
Authors: Shirahada Kunio , Zhang Yixin 
Published: November 2021

Purpose
This study aims to identify the counterproductive knowledge behavior (CKB) of volunteers in nonprofit organizations and its influencing factors, based on the theories of planned behavior and well-being.

Design/methodology/approach
An online survey was used to collect 496 valid responses. A structural equation model was constructed, and the relationships among the constructs were estimated via the maximum likelihood method. To analyze the direct and indirect effects, 2,000 bootstrapping runs were conducted. A Kruskal-Wallis test was also conducted to analyze the relationship between the variables.

Findings
A combination of organizational factors and individual attitudes and perceptions can be used to explain CKB. Insecurity about knowledge sharing had the greatest impact on CKB. A competitive organizational norm induced CKB while a knowledge-sharing organizational norm did not have a significant impact. Further, the more self-determined the volunteer activity was, the more the CKB was suppressed. However, well-being did not have a significant direct effect. Volunteers with high levels of well-being and self-determination had significantly lower levels of insecurity about knowledge sharing compared to those who did not.

Practical implications
Well-being arising from volunteering did not directly suppress CKB. To improve organizational efficiency by reducing CKB, nonprofit organization managers should provide intrinsically motivating tasks and interact with the volunteers.

Originality/value
There is a lack of empirical research on CKB in volunteer organizations; therefore, the authors propose a new approach to knowledge management in volunteer activities.