bluuebunny/crossref_metadata_2025_split huggingface dataset:
Clinical significance and potential mechanisms of the RNA methyltransferase KIAA1429 in osteosarcoma
Authors: Sun Yu , Lei Yi-wu , Ji Shu-Fan , Wu Hao , Liu Yun , Wen Sheng-Lian , Wei Ming-Hui , Chen Gang , He Mao-Lin 
Published: February 2021

Abstract
Background: KIAA1429, a member of the RNA methyltransferase complex, is involved in cancer progression. However, the clinical significance of KIAA1429 in osteosarcoma (OS) and the underlying mechanisms by which it contributes to disease progression remain unclear. Methods: The clinical significance of KIAA1429 in OS was evaluated based on the RT-qPCR, microarray, RNA sequencing, and published data. Two lentivirus-mediated KIAA1429-targeting siRNA constructs were transfected into SW1353 cells, and CCK-8 and flow cytometry assays were applied to investigate the biological function of KIAA1429 in OS cells. KIAA1429-related genes in OS were identified from lists of co-expressed genes (CEGs) and differentially expressed genes (DEGs). Functional enrichment analysis was employed to explore the potential mechanisms by which KIAA1429 contributes to the progression of OS.Results: The mRNA expression of KIAA1429 was notably overexpressed in 250 OS samples than 71 non-cancer samples (SMD=0.74). SROC curve analysis showed that KIAA1429 exhibits diagnostic capacity between OS samples and non-cancer samples (AUC=0.83). Survival analysis showed that overexpression of KIAA1429 was associated with shorter overall survival time. Knocking down KIAA1429 reduced the level of m6A methylation, inhibited proliferation, and accelerated apoptosis in OS cells. A total of 395 KIAA1429-related genes were identified, and were enriched in the cell cycle pathway. Protein–protein interaction (PPI) network analysis showed that CDK1, CCNA2, and CCNB1 were KIAA1429-related genes that act as major network hubs in OS. Conclusion: KIAA1429 plays an oncogenic role in OS and may facilitate the progression of OS through a mechanism involving the regulation of CDK1, CCNA2, and CCNB1.
Protein Model Accuracy Estimation Empowered by Deep Learning and Inter-residue Distance Prediction in CASP14
Authors: Chen Xiao , Liu Jian , Guo Zhiye , Wu Tianqi , Hou Jie , Cheng Jianlin 
Published: February 2021

Abstract
The inter-residue contact prediction and deep learning showed the promise to improve the estimation of protein model accuracy (CASP13) in the 13th Critical Assessment of Protein Structure Prediction (CASP13). During the 2020 CASP14 experiment, we developed and tested several EMA predictors that used deep learning with the new features based on inter-residue distance/contact predictions as well as the existing model quality features. The average global distance test (GDT-TS) score loss of ranking CASP14 structural models by three multi-model MULTICOM EMA predictors (MULTICOM-CONSTRUCT, MULTICOM-AI, and MULTICOM-CLUSTER) is 0.073, 0.079, and 0.081, respectively, which are ranked first, second, and third places out of 68 CASP14 EMA predictors. The single-model EMA predictor (MULTICOM-DEEP) is ranked 10th place among all the single-model EMA methods in terms of GDT-TS score loss. The results show that deep learning and contact/distance predictions are useful in ranking and selecting protein structural models.
Circulating Immune Biomarkers in Peripheral Blood Correlate With Clinical Outcomes in Advanced Breast Cancer
Authors: Palazón-Carrión Natalia , Jiménez-Cortegana Carlos , Sánchez-León M. Luisa , Henao-Carrasco Fernando , Nogales-Fernández Esteban , Chiesa Massimo , Caballero Rosalía , Rojo Federico , Nieto-García María-Adoración , Sánchez-Margalet Víctor , Cruz-Merino Luis de la 
Published: February 2021

Abstract
Identification of the different elements intervening at the immunoedition process, in each body compartment, seems key to explain clinical evolution in several tumor types. In this study, a set of immune biomarkers (myeloid derived suppressor cells, regulatory T cells, and OX40+ and PD-1+ T lymphocytes counts) in peripheral blood of patients diagnosed with advanced breast cancer, were analyzed prior to and along the implementation of first line antineoplastic therapy. Subsequently, a formal comparison between groups with clinical benefit versus progression of disease and with a healthy women cohort was executed. Results reflected that patients basally showed higher levels of myeloid derived suppressor cells (35.43, IR=180.73 vs 17.53, IR=16.96 cells/μl; p=0.001) and regulatory T cells (32.05, IR=29.84 vs 22.61, IR=13.57 cells/μl; p=0.001) in comparison with healthy women. Furthermore, after therapy, an increase in the number of activated T lymphocytes (expressing OX40), and a decrease of immune inhibitory cells (MDSCs, and Tregs), and the number of inhibited (or exhausted) T lymphocytes (expressing PD-1), could be ascertained in patients with clinical benefit (p≤0.001). The opposite trend was observed in the case of disease progression. These findings suggest that some critical immune elements can be easily detected and measured in peripheral blood, which open a new opportunity for translational research, as they seem to be clearly correlated with clinical evolution, at least in ABC.
Implementation Strategies and Outcomes for Occupational Therapy in Adult Stroke Rehabilitation: A Scoping Review
Authors: Murrell J. Edward , Pisegna Janell L. , Juckett Lisa A. 
Published: February 2021

Abstract
BackgroundEvery year, millions of people worldwide experience a stroke. Given the degree of physical, cognitive, visual, and behavioral impairments post-stroke, stroke survivors often encounter occupational therapy practitioners in the rehabilitation practice settings. Although evidence-based occupational therapy practices have been well-established in the stroke literature, it remains unclear what strategies have been used to promote these practices' effective implementation in the real-world context. The present review identifies these strategies and the extent to which researchers have measured implementation outcomes. MethodsA scoping review protocol was developed to assess the breadth and depth of occupational therapy literature examining implementation strategies and outcomes in the stroke rehabilitation field. Four electronic databases and two peer-reviewed implementation science journals were searched to identify studies meeting inclusion criteria. Two reviewers applied the inclusion parameters and consulted with a third reviewer, as needed, to achieve consensus. The Expert Change guided synthesis of review findings for Implementing Change project and the Implementation Outcomes Framework. ResultsThe initial search yielded 1219 studies, and 26 were included in the final review. A total of 48 out of 73 discrete implementation strategies were deployed in the included studies. The most used implementation strategies were “distribute educational materials,” “assess for readiness and identify barriers and facilitators,” and “conduct educational outreach visits.” “Adoption” was the most frequently measured implementation outcome, while “cost” was not measured in any included studies. Eleven studies reported findings to support the effectiveness of their implementation strategy or strategies; eleven studies reported inconclusive findings, and four studies found that their strategies did not lead to improved implementation outcomes. ConclusionsThis scoping review identified occupational therapy literature examining implementation strategies and outcomes in the context of stroke rehabilitation. With the growth of the stroke survivor population, the occupational therapy profession must identify effective strategies that promote the uptake of evidence-based practices into routine stroke care. Occupational therapy researchers and practitioners are encouraged to collaborate to develop and deploy implementation strategies responsive to known implementation barriers and facilitators in the stroke rehabilitation setting.
Tool Wear Inspection and Classiﬁcation by Artiﬁcial Neural Network Based on Vision System and Cutting Force Signal.
Authors: Abdeltawab Ahmed , Zhang Xi , Zhang Longjia , Li Chuanjun 
Published: February 2021

Abstract
The current work focuses on the cutting tool condition monitoring of end milling based on direct and indirect approach in machining AISI H13 alloy steel. Indirect process parameters such as cutting force signals are measured as responses using force sensor. In order to successfully inspect the milling tool life online for direct approaches, an automated machine vision system was used for tool condition monitoring. The image processing algorithms are developed to extract different features of rotating milling tool. A detection and compensation system for tool wear based on machine vision is designed. Feedforward Back-Propagation Neural Network applied for tool wear classification developed based on many force features. Ten time-domain features extracted and the sensitive features is determined based on Pearson’s correlation coefficient. I-kaz method which integrates between kurtosis and standard deviation is added as input feature with the ten time-domain features. A strong correlation is established between most of time-domain features and tool wear with high correlation coefficient. ANN model applied for classification tool states as normal and abnormal. Experiments with vision system have shown that area of wear at bottom and ﬂank is suitable to inspect in-process. Actual measurements of the tool wear stages are possible to identify the abnormality in cutting using vision system. ANN model showed superior results for tool states classification. The mean squared Error (MSE) for classification model was less than 6.7E-09 and R equal to 1. The model can be used to construct fault estimation mode for tool wear online classification and inspection.
High molecular weight glutenin gene diversity in Aegilops tauschii demonstrates unique origin of superior wheat quality
Authors: Delorean Emily , Gao LiangLiang , Lopez Jose Fausto Cervantes , Consortium The Open Wild Wheat , Wulff Brande , Ibba Maria Itria , Poland Jesse 
Published: February 2021

Abstract
Central to the diversity of wheat products was the origin of hexaploid bread wheat, which added the D-genome of Aegilops tauschii to tetraploid wheat giving rise to superior dough properties in leavened breads. The polyploidization, however, imposed a genetic bottleneck, with only limited diversity introduced in the wheat D-subgenome. To understand genetic variants for quality, we sequenced 273 accessions spanning the known diversity of Ae. tauschii. We discovered 45 haplotypes in Glu-D1, a major determinant of quality, relative to the two predominant haplotypes in wheat. The wheat allele 2+12 was found in Ae. tauschii Lineage 2, the donor of the wheat D-subgenome. Conversely, the superior quality wheat allele 5+10 allele originated in Lineage 3, a recently characterized lineage of Ae. tauschii, showing a unique origin of this important allele. These two wheat alleles were also quite similar relative to the total observed molecular diversity in Ae. tauschii at Glu-D1. Ae. tauschii is thus a reservoir for unique Glu-D1 alleles and provides the genomic resource to begin utilizing new alleles for end-use quality improvement in wheat breeding programs.
Passive repetitive stretching increases skeletal muscle mass and myofiber cross-sectional area in senescence-accelerated model mouse
Authors: Wang Yumin , Ikeda Satoshi , Ikoma Katsunori 
Published: February 2021

Abstract
Mechanical stimulation has benefits for muscle mass and function. Passive stretching is widely performed in clinical rehabilitation medicine. However, the hypertrophic effects of passive repetitive stretching on senescent skeletal muscles against muscle atrophy remain unknown. We used senescence-accelerated model SAM-P8 mice. The gastrocnemius muscle was passively repetitive stretched by manual ankle dorsiflexion for 15 min, 5 days a week for 2 weeks under deep anesthesia. We examined the effects of passive stretching on muscle mass, myofiber cross-sectional area, muscle fiber type and composition, satellite cell content, mRNA expression of the signaling pathways involved in muscle protein synthesis, muscle-specific ubiquitin ligases, and myogenic regulatory factors. The gastrocnemius muscle weight of the stretched side increased compared with that of the unstretched side. In addition to the increase in muscle mass, muscle fiber cross-sectional area of the stretched side was greater than that of the unstretched side. Passive repetitive stretching significantly increased the mRNA expression level of Akt, p70S6K, 4E-BP1, Myf5, myogenin, MuRF1. Passive repetitive stretching promoted skeletal muscle mass and myofiber cross-sectional area in SAM-P8 mice. These hypertrophic observations are attributable to the stretch-activated signaling pathways involved in protein turnover. These findings are applicable to clinical muscle strengthening and sarcopenia prevention.
A study on the preparation, evaluation of biological characteristics, and preliminary imaging of [188Re]Re-ibandronate
Authors: Xu Tingting , Wang Yingwei , Chen Zan , Liu Hanxiang , Yang Songsong , Liu Guangfu , Zhao Yan , Fu Wenhui , Liu Lin , Xiang Ke , Peng Dengsai , Chen Yue 
Published: February 2021

Abstract
Background

Bone is a common site of metastasis of malignant tumor. Several radiopharmaceuticals are available to relieve bone pain in patients with cancer. However, there is still a need to to investigate easily accessible and high bone affinity radiopharmaceuticals. Radionuclide 188Re has an advantage in this regard because of its commercial extraction from 188W/188Re generators. It can be used on demand and is cost-effective. The first-generation bisphosphonate hydroxyethylidene diphosphonate (HEDP) is the commonly used bisphosphonate for 188Re-labelling. And the third-generation bisphosphonates ibandronate (IBA) has higher bone affinity and ability to inhibit bone resorption than HEDP. However, there have been no reports on 188Re-labelling with IBA. We used IBA and 188Re for radiolabeling to develop and evaluate a novel type of bone-seeking radiopharmaceutical.
Results

We successfully prepared [188Re]Re-IBA in > 95% RCP. The optimum preparation conditions were as follows: IBA, 1.2–1.6 mg; ascorbic acid, 0.20–0.35 mg; stannous chloride, 0.14–0.18 mg; potassium perrhenate, 0.005–0.009 mg; and [188Re]ReO4− activity, 18.5–55 MBq, were reacted for 30 min at 95℃ and pH = 2. [188Re]Re-IBA demonstrated good stability in vitro, high plasma protein binding rate, and good hydrophilicity. The in vivo distribution of mice and bone imaging in New Zealand rabbits showed high bone uptake, long retention time, rapid blood clearance, and relatively low soft tissue uptake.
Conclusion

Our study encompassed the successful preparation of [188Re]Re-IBA, a novel bone-seeking radiopharmaceutical, and confirmed it has great potential for the treatment of bone pain and imaging monitoring.
The Treatment Zone Size and Its Decentration Influence Axial Length Grow in Children With Orthokeratology Treatment
Authors: Lin Weiping , Li Na , Gu Tianpu , Tang Chunyu , Du Bei , Liu Guihua , wei ruihua 
Published: February 2021

Abstract
Background: To investigate whether the treatment zone size (TZS) and treatment zone decentration (TZD) will affect the axial length growth in myopic children undergoing orthokeratology treatment.Methods: A self-controlled retrospective study was conducted on 352 children who met the inclusion criteria. Axial length was measured before and at 12 months after the initial lens wear. Corneal topography was measured at baseline and at each follow-up after lens wear. The Corneal topography obtained from the 12-month visit was used to quantify TZS and TZD for each subject. Cycloplegic refraction was required for all subjects before fitting the orthokeratology lenses. Results: Axial length growth was significantly associated with age, baseline spherical equivalent (SE), TZS, and TZD with univariate linear regression. In groups with both small and large TZS, axial length growth was significantly decreased with large TZD (both P < 0.001). In groups with both small and large TZD, axial length growth was significantly decreased with small TZS (P = 0.027 for small TZD, P = 0.011 for large TZD). Age, SE, and TZD were significantly associated with axial length growth in multiple regression (all P < 0.001). Conclusion: Relatively smaller TZS and larger TZD may be beneficial in slowing myopia progression in children with orthokeratology treatment.
Revisit the Practice of Lymph Node Biopsy in Patients Diagnosed as Ductal Carcinoma In Situ Before Operation: A Retrospective Analysis of 682 Cases and Evaluate the Role of Breast MRI
Authors: Lai Hung-Wen , Chang Yi Lin , Chen Shou-Tung , Chang Yu-Chun , Wu Wen-Pei , Chen Dar-Ren , Kuo Shou-Jen 
Published: February 2021

Abstract
Background: The optimal axillary lymph node (ALN) management strategy in patients diagnosed with ductal carcinoma in situ (DCIS) preoperatively remains controversial. The value of breast magnetic resonance imaging (MRI) to predict ALN metastasis pre-operative DCIS patients were evaluated. Methods: Patients with primary DCIS with or without pre-operative breast MRI evaluation and underwent breast surgery were recruited from single institution. The value of breast MRI for ALN evaluation, predictors of breast & ALN surgeries, upgrade from DCIS to invasive cancer, and ALN metastasis were analyzed.Results: A total of 682 cases with pre-operative diagnosis of DCIS were enrolled in current study. The rate of upgrade to invasive cancer were found in 34.2% of specimen, and this upgrade rate is 23% for patients received breast conserving surgery and 40.7% for mastectomy (p<0.01). Large pre-operative imaging tumor size and post-operative invasive component were risk factors to ALN metastasis. Breast MRI had 53.8% sensitivity, 77.8% specificity, 14.9% positive predictive value, 95.9% negative predictive value (NPV), and 76.2% accuracy to predict ALN metastasis in pre-OP DCIS patients. In MRI node negative breast cancer patients with MRI tumor size < 3cm, the NPV was 96.4%, and all these false negative cases were N1. Pre-OP diagnosed DCIS patients with MRI tumor size <3 cm and node negative suitable for BCS could safety omit SLNB if whole breast radiotherapy is to be performed.Conclusion: Breast MRI had high NPV to predict ALN metastasis in pre-OP DCIS patients, which is useful and could be provided as shared decision-making reference.
Exosomes from human urine-derived stem cells ameliorate particulate polyethylene-induced osteolysis
Authors: Li Hui , Fan Xiaolei , Wang Yinan , Lu Wei , Wang Haoyi , Liao Runzhi , Zeng Min , Yang Junxiao , Xie Jie 
Published: February 2021

Abstract
Background: Wear particle-induced periprosthetic osteolysis is a common long-term complication of total joint arthroplasty, and represents the major cause of aseptic loosening and subsequent implant failure. Currently, there are no effective therapeutic options to prevent osteolysis from occurring and often need revision surgery. Exosomes are important nano-sized paracrine mediators of intercellular communications and can be directly utilized as therapeutic agents for tissue repair and regeneration. Here, we explored the therapeutic potential of exosomes from human urine-derived stem cells (USC-Exos) in preventing wear particle-induced osteolysis.Methods: USCs were characterized by flow cytometry and multiple differentiation potential. USC-Exos were identified by transmission electron microscopy (TEM), dynamic light scattering (DLS) and western blotting (WB). The impact of USC-Exos on osteoblastic differentiation of bone marrow mesenchymal stromal cells (BMSCs) and osteoclastogenesis of RAW264.7 cells were verified in vitro. The effects of USC-Exos on ultra high molecular weight PE (UHMWPE)-induced murine calvarial osteolysis model were tested to evaluate bone mass, inflammation, osteogenic and osteoclastic activities.Results: USCs were positive for CD44, CD73, CD29 and CD90, but negative for CD34 and CD45. USCs were able to differentiate into osteogenic, chondrogenic, and adipogenic cells. USC-Exos exhibited a round-shaped morphology with a double-layered membrane structure and positive for CD63 and TSG101, negative for Calnexin. In vitro, USC-Exos could promote the osteogenic differentiation of BMSCs, reduces the production of proinflammatory factors in macrophages and suppresses their osteoclastic abilities. In vivo, injection of USC-Exos into the center of the calvariae caused less inflammatory cytokine generation and less osteolysis compared to control and significantly enhanced the bone formation.Conclusions: Our findings demonstrate that USC-Exos can prevent UHMWPE-induced osteolysis by inciting less inflammatory, inhibiting bone resorption and stimulating bone formation. USC-Exos may represent a potential natural agent for the treatment of periprosthetic osteolysis and to obtain therapeutic exosomes for osteolytic treatment, aseptic loosening patients may just need to collect a certain volume of their own urine to harvest USCs and USC-Exos.
The Effect of Increased Methane Flow Rate on Electronic Correlation of Amorphous Silicon Carbon (a-SiC: H)
Authors: Prayogi soni , Sholehah Ayunis , Cahyono Yoyok , D Darminto 
Published: February 2021

Abstract
In this study, we report for the first time that the addition of methane (CH4) flow rate in the p-type a-SiC: H layer greatly affects the electronic correlation in increasing the conversion efficiency of solar cells. The a-SiC: H p-type layer was grown using Plasma Enhanced Chemical Vapor Deposition (PECVD) on Indium Tin Oxide (ITO) substrate with various methane flow rates. The a-SiC: H p-type layer was characterized including the complex dielectric properties and the complex refractive index using Ellipsometric Spectroscopy (ES), while the surface roughness morphology was used Atomic Force Microscopy (AFM). In sample P-2 there is a change in the form of a decrease in the value of the refractive index < n > and the E0 energy in the lower energy compared to the P-1 sample with a change of 0.3 eV, an increase in the optical gap and a decrease in the value of the real and imaginary dielectric function. While the influence of an increase in the carbon composition of the amorphous network order shows the addition of amorphous tissue disorder. Our results, show that the optical magnitude of the p-type a-SiC: H layer is not only affected by the amount of carbon in the film but also the hydrogen which is thought to contribute.
Prognostic Significance of Neutrophil-Lymphocyte Ratio and Platelet-Lymphocyte Ratio in Metastatic Colorectal Cancer
Authors: Bulut Gülcan , Özdemir Zehra Narlı 
Published: February 2021

Abstract
There are many studies on biomarkers for prognosis in the treatment of metastatic colorectal cancer. Neutrophil-lymphocyte radio (NLR) and Platelet-lymphocyte radio (PLR) are of interest with studies revealing the relationship between inflammatory biomarkers and cancer. Our study is a retrospective file study and the contribution of NLR and PLR to progression-free survival (PFS) and overall survival(OS) before first line chemotherapy was investigated regardless treatment. The cut off values of NLR and TLR were determined using ROC curve analysis. NLR and PLR was divided into two groups according to the cut-off points. OS and PFS associated with NLR and TLR were performed by Kaplan-Meier method. In our study, we could not demonstrate the prognostic potential of pre-treatment NLR and PLR in patients with mCRC treated with first-line chemotherapy. Our study was showed that the use of these biomarkers in mCRC is limited.
Biocatalytic Reductive Amination- Discovery to Commercial Manufacturing applied to Abrocitinib JAK1 inhibitor
Authors: Kumar Rajesh , Karmilowicz Michael , Burke Dylan , Burns Michael , Clark Leslie , Connor Christina , Cordi Eric , Do Nga , Doyle Kevin , Hoagland Steve , Lewis Chad , Mangan David , Martinez Carlos , McInturff Emma , Meldrum Kevin , Pearson Robert , Steflik Jeremy , Rane Anil , Weaver John 
Published: February 2021

Abstract
The authors have requested that this preprint be removed from Research Square.