bluuebunny/crossref_metadata_2025_split huggingface dataset:
Efficacy of Petiveria Alliacea (Linneaus) leaf and root bark powder in the control of filariasis vector Culex Quinquefasciatus
Authors: Akintan Michael Olarewaju , Akinneye Joseph Onaolapo , Ilelakinwa Oluwatosin Betty 
Published: January 2021

Abstract
Background
Mosquitoes are vectors of parasitic diseases such as malaria, lymphatic filariasis, yellow fever, and dengue fever among others. They are well known as public enemies for their noise nuisance, biting annoyance, sleeplessness, allergic reactions, and diseases transmission during the biting and feeding activities. This then necessitate the search for insecticides of plant origin which are bio-degradable, non-toxic, and readily available for man use.

Result
This study, evaluated the fumigant efficacy of the powder of P. alliacea to control the adult stage of Culex mosquito. Powder of Petiveria alliacea were administered at different dose of (1 g, 2 g, 3 g, 4 g, and 5 g), respectively. Result obtained shows the fumigant effect of the powder were effective with percentage mortality of 18.33–60.00% for the leaf powder and 23.30–71.60% for the root powder within 2 h post-treatment period (P < 0.05). The synergistic effect of the leaf and root powder was also investigated. The lethal dosage (LD50) of the leaf, root, and synergistic effect of leaf and root bark powder required to kill 50% of the adult Culex quinquefasciatus was 3.76 g, 2.86 g, and 2.63 g, respectively. However, 25.06 g, 15.25 g, and 12.94 g of the leaf, root, and leaf and root powder were required to kill 90% (LD90) after a 2-h exposure period.

Conclusion
These finding suggested P. alliacea powder could be a good source of insecticide which may be used for the production of biopesticides. The present findings have important implications in the practical control of adult mosquito by using botanical insecticides. These plant powders are easy to prepare, inexpensive, and safe for use in mosquito control.
Repetitive transcranial magnetic stimulation as a prophylactic treatment in migraine
Authors: Hammad Azza B. , Elsharkawy Rasha E. , Abdel Azim Ghada S. 
Published: January 2021

Abstract
Background
Clinical applications of transcranial magnetic stimulation (TMS) have shown promising results in the treatment of headache disorders, with migraine being one of the most encountered.

Objective
To assess the role of low-frequency repetitive transcranial magnetic stimulation as a preventive treatment of migraine (with and without aura) and correlate the results with the serum level of the inflammatory biomarker (neurokinin A).

Methods
Forty patients, with age ranging from 15 to 55 years, diagnosed with migraine (30 migraine without aura and 10 with aura) and 20 apparently healthy individuals, who were age and sex matched with the patient group, were included in this study.
A low-frequency (1 Hz) rTMS protocol was applied for all patients for five consecutive days interictally. Assessment of pain intensity using visual analogue scale and frequency and duration of attacks as well as number of pills taken by patients as an abortive treatment according to the Basic Diagnostic Headache Diary for 4 weeks before and 4 weeks after TMS sessions was done. In addition, the Migraine Disability Assessment scale (MIDAS) was applied to assess the severity and degree of disability caused by migraine.
Measurement of neurokinin A serum level was done by using ELISA for all patients before and after TMS and for control group once.

Results
There was a significant reduction in pain intensity, frequency and duration of migraine attacks, migraine disability scores, and number of pills taken as abortive treatment for attacks after rTMS (P < 0.001). Also, serum level of neurokinin A in the patients was significantly reduced after rTMS (P < 0.001).

Conclusion
Low-frequency rTMS is an effective prophylactic treatment for migraine with and without aura.
A review on liquid chromatographic methods for the bioanalysis of atorvastatin
Authors: Wadhwa Karan , Rana A. C. 
Published: January 2021

Abstract
Background
The unsatisfied clinical need has encouraged the development and validation of bioanalytical procedures for the quantification of drugs in biological samples because the monitoring of drug concentrations helps in personalizing the patient’s pharmacotherapy, assessing the adherence to therapy, and is also extensively useful for pharmacokinetics and drug-drug interactions studies.

Main Body
The present review aimed to provide insightful information about the various liquid chromatographic methods developed till 2019 for the analysis and quantification of atorvastatin, its metabolites, and co-administered drugs in the various biological matrices like the serum, plasma, and urine with special emphasis on sample preparation techniques applied before chromatographic analysis along with different chromatographic conditions and their validation data. A total of 88 published papers that have used liquid chromatography techniques to quantify atorvastatin in biological fluids are included in the study. Out of the total reported liquid chromatographic methods, 34% used UV spectrophotometer as a detector, and 55% used MS/MS as a detector. Whereas 38% of them used protein precipitation procedure, 33% applied liquid-liquid extraction approach, and 12% employed solid-phase extraction technique for sample preparation.

Conclusion
In the last decade, numerous bioanalytical procedures have been developed for the quantification of atorvastatin in different biological samples using liquid chromatographic techniques. Moreover, advancement in technology developed several new and advanced sample preparation approaches like dispersive liquid-liquid extraction, microextraction by packed sorbent, which have high recovery rates than conventional procedures. Thus, the summarized review may be consulted as an informative tool to support the optimization of new bioanalytical methods for the quantification of atorvastatin.
Comparative evaluation of intranasally delivered quetiapine loaded mucoadhesive microemulsion and polymeric nanoparticles for brain targeting: pharmacokinetic and gamma scintigraphy studies
Authors: Shah Brijesh , Khunt Dignesh , Misra Manju 
Published: January 2021

Abstract
Background
Treatment in neurological disorders like schizophrenia requires continuous presence of drug in the brain for a prolonged period of time to achieve an effective therapeutic response. Delivery of antipsychotic drug quetiapine in the form of conventional delivery systems suffers from low oral bioavailability, first-pass metabolism, and frequent dosing. In addition to that biological obstacles present at the brain interface also hinders the transport of quetiapine across the brain. In the present study, nasal delivery of quetiapine loaded nanoparticles and microemulsion formulation were designed to evaluate their individual in vivo potential to achieve brain targeting. Chitosan-based polymeric nanoparticles and mucoadhesive microemulsion systems were developed through ionic gelation and water titration method respectively.

Results
Microemulsion showed globule size lower than 50 nm with 95% drug loading while, nanoparticles exhibited 65% drug loading with particle size of 131 nm. Nasal diffusion study showed highest diffusion with chitosan-based mucoadhesive microemulsion over nanoparticles suggesting permeation-enhancing effects of chitosan. Due to the overall hydrophilic nature, quetiapine-loaded nanoparticles could not diffuse superiorly across nasal mucosa, hence, showed 1.3 times lesser diffusion compared to mucoadhesive microemulsion. Pharmacokinetics in rats showed highest brain concentration and 1.9-folds higher nasal bioavailability with mucoadhesive microemulsion over nanoparticles suggesting direct brain transport through olfactory route bypassing blood-brain barrier.

Conclusion
Higher quetiapine transport with mucoadhesive microemulsion suggested that synergistic effects like tight junction modulation by chitosan and unique composition facilitating smaller globule size could be responsible for higher brain transport. Imaging study by gamma scintigraphy also supported pharmacokinetic outcomes and concluded that mucoadhesive microemulsion could be a promising nanocarrier approach for non-invasive nose to brain delivery.

Graphical abstract
Development of reverse phase high-performance liquid chromatographic method for the estimation of HIV non-nucleoside reverse transcriptase inhibitor drug efavirenz in the rat brain
Authors: Kakad Smita Prakash , Kshirsagar Sanjay Jayprakash 
Published: January 2021

Abstract
Background
The brain is the potential viral reservoir, and estimating the antiviral drug concentration in the brain is a hurdle to the researchers as very few animal models are available for this study. The objective of the study was to develop and validate the RP-HPLC method for the estimation of antiviral drug efavirenz (EFV) in the brain of healthy Wistar rats. EFV was the first-line antiretroviral medication. The optimized HPLC condition used for the analysis had the mobile phase methanol to water (9:1) ratio. The flow rate was set at 0.8 mL/min, while the detection wavelength was 248 nm.

Results
The retention time was found to be 5.7 min, and the % RSD was found within the limit. Recovery was found to be nearly 78%. The validation results were found to be within the limit range; hence, the obtained method was accurate, specific, rapid, and repeatable for estimation of EFV in the brain.

Conclusion
This method for estimation of EFV in the rat brain can be applicable for pharmacokinetic and toxicology study of EFV in the brain after administration of EFV to rats.

Graphical abstract
.
Environmental hazards and demographic and clinical data of childhood interstitial lung diseases in a tertiary institute in Egypt
Authors: Ishak Sally Raafat , Hassan Azza Mohammed , Kamel Terez Boshra 
Published: January 2021

AbstractBackgroundThe incidence of childhood interstitial lung diseases increased in the last years in Egypt. Changes in environmental and climatic conditions may be contributing factors. Also, raising birds at home increased in the past years due to financial issues. Other environmental factors include increased industries, traffic, and pollution.Our study aimed to assess the environmental hazards and the severity of childhood interstitial lung diseases.ResultsSixty-five percent of patients with childhood interstitial lung diseases (chILD) were exposed to cigarette smoke; 45% were exposed to birds, 30% to industrial wastes, 20% to grass and pesticides, and 10% to animals.ConclusionsExposure to cigarette smoke and birds increases the risk of development of chILD.
How gas-hydrate saturation and morphology control seismic attenuation: A case study from the south Hydrate Ridge
Authors: Ji Aoshuang , Zhu Tieyuan , Marín-Moreno Héctor , Lei Xiong 
Published: January 2021

Prior studies have shown an ambiguous relationship between gas-hydrate saturation and seismic attenuation in different regions, but the effect of gas-hydrate morphology on seismic attenuation of hydrate-bearing sediments was often overlooked. We have combined seismic data with rock-physics modeling to elucidate how gas-hydrate saturation and morphology may control seismic attenuation. To extract P-wave attenuation, we process the vertical seismic profile data within a frequency range of 30–150 Hz and the sonic logging data within 10–15 kHz from three wells in the south Hydrate Ridge, offshore Oregon (USA), collected during Ocean Drilling Program Leg 204 in 2000. We calculate the P-wave attenuation using spectral matching and centroid frequency shift methods, and we use Archie’s relationship to derive gas-hydrate saturation from the resistivity data above the bottom-simulating reflection at the same wells. To interpret the observed seismic attenuation in terms of the effects of the gas-hydrate saturation and the morphology, we use the hydrate-bearing effective sediment rock-physics model. By comparing the observed and model-predicted attenuation values, we infer that (1) seismic attenuation appears to not be dominated by any single factor — instead, its variation is likely governed by the gas-hydrate saturation and the morphology; (2) the relationship between seismic attenuation and gas-hydrate saturation varies with different hydrate morphologies; (3) the squirt flow, occurring at different compliances of adjacent pores driven by pressure gradients, may be responsible for the significantly large or small attenuation over a broad frequency range.
Smartphone Health Assessment for Relapse Prevention (SHARP): a digital solution toward global mental health
Authors: Rodriguez-Villa Elena , Mehta Urvakhsh Meherwan , Naslund John , Tugnawat Deepak , Gupta Snehil , Thirtalli Jagadisha , Bhan Anant , Patel Vikram , Chand Prabhat Kumar , Rozatkar Abhijit , Keshavan Matcheri , Torous John 
Published: January 2021

Background
Predicting and preventing relapse presents a crucial opportunity and first step to improve outcomes and reduce the care gap for persons living with schizophrenia. Using commercially available smartphones and smartwatches, technology now affords opportunities to capture real-time and longitudinal profiles of patients’ symptoms, cognition, physiology and social patterns. This novel data makes it possible to explore relationships between behaviours, physiology and symptoms, which may yield personalised relapse signals.

Aims
Smartphone Health Assessment for Relapse Prevention (SHARP), an international mental health research study supported by the Wellcome Trust, will inform the development of a scalable and sharable digital health solution to monitor personal risk of relapse. The resulting technology will be studied toward predicting and preventing relapse among individuals diagnosed with serious mental illness.

Method
SHARP is a two-phase study with research sites in Boston, Massachusetts, and Bangalore and Bhopal, India. During phase 1, focus groups will be conducted at each study site to collect feedback on the design and features available on mindLAMP, a digital health platform. Individuals with serious mental illness will use mindLAMP for the duration of a year during phase 2.

Results
The results of the research outlined in this protocol will guide the development of technology and digital tools to help address pervasive challenges in global mental health.

Conclusions
The digital tools developed as a result of this study, and participants’ experiences using them, may offer insight into opportunities to expand digital mental health resources and optimize their utilisation around the world.
Benefits of using a photostimulable phosphor plate protective device
Authors: de Moura Graziela , Vizzotto Mariana Boessio , Tiecher Priscila Fernanda da Silveira , Arús Nádia Assein , Silveira Heraldo Luis Dias da 
Published: January 2021

Objectives: To develop and test a protective device (PD) to increase the resistance of photostimulable storage phosphor (PSP) plate to compressive load, and assess the resulting image quality. Methods: Two prototypes, polyvinylchloride sheets of 0.3 mm and 0.7 mm each, were developed for PSP plate size 2. The resistance to compressive load was tested using eight new PSPs divided into four test groups: (1) PSP, (2) PSP and paperboard protector, (3) PSP and 0.3 mm PD, and (4) PSP and 0.7 mm PD. The resulting images were analyzed by three oral radiologists, based on the consensus for image artifacts. Additionally, the objective image quality test was performed with four new PSPs, using an 8-step wedge aluminum scale. The mean gray values and standard deviation were measured in a total of 240 images, and the data were analyzed using analysis of variance with Bonferroni post-hoc test. Results: Artifacts were seen in the PSP control group starting at 40 n, and at 150 n, 175 n and 300 n in 0.3 mm PD, paperboard protector and 0.7 mm PD, respectively. Although there was no statistical difference among groups, there were differences between exposure times (0.06–0.25 s, 0.06–0.40 s, and 0.10–0.40 s). Scanning resolution of 20 lp/mm showed higher mean gray value than 25 and 40 lp/mm (p < 0.05) Conclusion: The developed PDs improved the PSP resistance to compressive forces, with low interference on the pixel gray values, regardless of exposure time and spatial resolution. Nevertheless, the 0.7 mm PD could withstand the maximum compressive load.
Mothers’ employment and child behaviour: new evidence for Scotland
Authors: Jacob Marita , Kühhirt Michael 
Published: January 2021

Given increasing maternal labour-market participation in many European countries, there is an ongoing scientific and public debate on the potential consequences for children’s development. Previous research has used both cross-sectional measures of maternal employment at a particular age of the child and measures capturing maternal employment history. Whereas the former approach cannot capture the cumulative impact of maternal employment on developmental outcomes, studies following the second approach have so far not accounted for the possibility that mothers may repeatedly change their labour-force participation in response to their children’s development or other dynamic context factors that are themselves affecting developmental outcomes.
The present study combines statistical techniques that can account for time-varying confounders with cumulative measurement of maternal employment to investigate its link with children’s behavioural problems around age eight. In addition, our study explores whether the effect of maternal employment history differs by mothers’ education. Using data from the Growing Up in Scotland study, we find that children’s behavioural problems around age eight are the less pronounced the more years their mothers have worked full-time or part-time. However, these associations reduced in size once we adjusted for potential confounders and they do not significantly differ between mothers with and without a tertiary degree. These results suggest that the association between maternal employment history and behavioural problems around age eight is mostly driven by confounding factors such as maternal education, child health and socio-economic status.
Identifying differences in gait adaptability across various speeds using movement synergy analysis
Authors: Ó’Reilly David , Federolf Peter 
Published: January 2021

Introduction
The aim of this study was to identify movement synergies during normal-walking that can differentiate healthy adults in terms of gait adaptability at various speeds. To this end, the association between movement synergies and lower-limb coordination variability or Deviation Phase (DP) was investigated. This study also investigated the moderating effect of movement synergies on the relationship between DP and the smoothness of arm-swing motion (NJI).

Method
A principal component analysis of whole-body marker trajectories from normal-walking treadmill trials at 0.8m/s, 1.2m/s and 1.6m/s was undertaken. Both DP and NJI were derived from approx. 8 minutes of perturbed-walking treadmill trials. Principal movement components, PMk, were derived and the RMS of the 2nd-order differentiation of these PMk (PAkRMS) were included as independent variables representing the magnitude of neuromuscular control in each PMk. Each PAkRMS were input into maximal linear mixed-effects models against DP and (DP x NJI) respectively. A stepwise elimination of terms and comparison of models using Anova identified optimal models for both aims.

Results
The principal movement related to the push-off mechanism of gait (PA4RMS) was identified as an optimal model and demonstrated a significant negative effect on DP however this effect may differ considerably across walking-speeds. An optimal model for describing the variance in (DP x NJI) included a fixed-effect of PA6RMS representing Right—Left side weight transfer was identified.

Interpretation
The hypotheses that individuals who exhibited greater control on specific kinematic synergies would exhibit variations during perturbed walking was substantiated. Supporting evidence for the role of movement synergies during the double-support phase of gait in proactively correcting balance was presented as well as the potential for this approach in targeted rehabilitation. The potential influence of leg dominance on gait adaptability was also discussed. Future studies should investigate further the role of walking-speed and leg dominance on movement synergies and look to generalize these findings to patient populations.
Towards remote monitoring in pediatric care and clinical trials—Tolerability, repeatability and reference values of candidate digital endpoints derived from physical activity, heart rate and sleep in healthy children
Authors: Kruizinga M. D. , Heide N. van der , Moll A. , Zhuparris A. , Yavuz Y. , Kam M. L. de , Stuurman F. E. , Cohen A. F. , Driessen G. J. A. 
Published: January 2021

Background
Digital devices and wearables allow for the measurement of a wide range of health-related parameters in a non-invasive manner, which may be particularly valuable in pediatrics. Incorporation of such parameters in clinical trials or care as digital endpoint could reduce the burden for children and their parents but requires clinical validation in the target population. This study aims to determine the tolerability, repeatability, and reference values of novel digital endpoints in healthy children.

Methods
Apparently healthy children (n = 175, 46% male) aged 2–16 were included. Subjects were monitored for 21 days using a home-monitoring platform with several devices (smartwatch, spirometer, thermometer, blood pressure monitor, scales). Endpoints were analyzed with a mixed effects model, assessing variables that explained within- and between-subject variability. Endpoints based on physical activity, heart rate, and sleep-related parameters were included in the analysis. For physical-activity-related endpoints, a sample size needed to detect a 15% increase was calculated.

Findings
Median compliance was 94%. Variability in each physical activity-related candidate endpoint was explained by age, sex, watch wear time, rain duration per day, average ambient temperature, and population density of the city of residence. Estimated sample sizes for candidate endpoints ranged from 33–110 per group. Daytime heart rate, nocturnal heart rate and sleep duration decreased as a function of age and were comparable to reference values published in the literature.

Conclusions
Wearable- and portable devices are tolerable for pediatric subjects. The raw data, models and reference values presented here can be used to guide further validation and, in the future, clinical trial designs involving the included measures.