bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mediation of Interleukin‐23 and Tumor Necrosis Factor–Driven Reactive Arthritis by Chlamydia‐Infected Macrophages in SKG Mice
Authors: Romand Xavier , Liu Xiao , Rahman M. Arifur , Bhuyan Zaied Ahmed , Douillard Claire , Kedia Reena Arora , Stone Nathan , Roest Dominique , Chew Zi Huai , Cameron Amy J. , Rehaume Linda M. , Bozon Aurélie , Habib Mohammed , Armitage Charles W. , Nguyen Minh Vu Chuong , Favier Bertrand , Beagley Kenneth , Maurin Max , Gaudin Philippe , Thomas Ranjeny , Wells Timothy J. , Baillet Athan 
Published: January 2021

ObjectiveZAP‐70W163C BALB/c (SKG) mice develop reactive arthritis (ReA) following infection with Chlamydia muridarum. Since intracellular pathogens enhance their replicative fitness in stressed host cells, we examined how myeloid cells infected with C muridarum drive arthritis.MethodsSKG, Il17a‐deficient SKG, and BALB/c female mice were infected with C muridarum or C muridarum luciferase in the genitals. C muridarum dissemination was assessed by in vivo imaging or genomic DNA amplification. Macrophages were depleted using clodronate liposomes. Anti–tumor necrosis factor (anti‐TNF) and anti–interleukin‐23p19 (anti–IL‐23p19) were administered after infection or arthritis onset. Gene expression of Hspa5, Tgtp1, Il23a, Il17a, Il12b, and Tnf was compared in SKG mice and BALB/c mice.ResultsOne week following infection with C muridarum, macrophages and neutrophils were observed to have infiltrated the uteri of mice and were also shown to have carried C muridarum DNA to the spleen. C muridarum load was higher in SKG mice than in BALB/c mice. Macrophage depletion was shown to reduce C muridarum load and prevent development of arthritis. Compared with BALB/c mice, expression of Il23a and Il17a was increased in the uterine and splenic neutrophils of SKG mice. The presence of anti–IL‐23p19 during infection or Il17a deficiency suppressed arthritis. Tnf was overexpressed in the joints of SKG mice within 1 week postinfection, and persisted beyond the first week. TNF inhibition during infection or at arthritis onset suppressed the development of arthritis. Levels of endoplasmic reticulum stress were constitutively increased in the joints of SKG mice but were induced, in conjunction with immunity‐related GTPase, by C muridarum infection in the uterus.ConclusionC muridarum load is higher in SKG mice than in BALB/c mice. Whereas proinflammatory IL‐23 produced by neutrophils contributes to the initiation of C muridarum–mediated ReA, macrophage depletion reduces C muridarum dissemination to other tissues, tissue burden, and the development of arthritis. TNF inhibition was also shown to suppress arthritis development. Our data suggest that enhanced bacterial dissemination in macrophages of SKG mice drives the TNF production needed for persistent arthritis.
SO2 Resisting Pd‐doped Pr1‐xCexMnO3 Perovskites for Efficient Denitration at Low Temperature
Authors: Ma Linghui , Ma Chenglong , Xie Tianying , Cao Limei , Yang Ji 
Published: January 2021

AbstractH2‐SCR is served as the promising technology for the controlling of NOx emission, and the Pd‐based derivative catalyst exhibited high NOx reduction performance. Effectively regulating the electronic configuration of the active component is favorable to the rational optimization of noble Pd. In this work, a series of Pr1‐xCexMn1‐yPdyO3@Ni were successfully synthesized and exhibited superior NO conversion efficiency at low temperatures. 92.7 % conversion efficiency was achieved at 200 °C over Pr0.9Ce0.1Mn0.9Pd0.1O3@Ni in the presence of 4 % O2 with a GHSV of 32000 h−1. Meanwhile, the outstanding performance was obtained in the resistance to SO2 (200 ppm) and H2O (8 %). Deduced from the results of XRD, Raman, XPS, and H2‐TPR, the modification of d orbit states in palladium was confirmed originating from the incorporation in the B site of Pr0.9Ce0.1Mn0.9Pd0.1O3. The existence of higher valence (Pd3+ and Pd4+) than the bivalence in Pr0.9Ce0.1Mn0.9Pd0.1O3 catalyst was evidenced by XPS analysis. Our research provides a new sight into the H2‐SCR through the higher utilization of Pd.
Identification of carbonylated proteins from monocytic cells under diabetes‐induced stress conditions
Authors: Nair Divya , Nedungadi Divya , Mishra Nandita , Nair Bipin Gopalakrishnan , Nair Sudarslal Sadasivan 
Published: January 2021

AbstractDiabetes is a metabolic disorder characterized by the presence of elevated glucose in the blood and enhanced oxidative stress. It affects the cellular homeostasis that leads to the development of micro‐and macro‐vascular complications. Monocytes are the primary immune cells present in the circulatory system. Under high‐glucose conditions, the cells undergo oxidative stress and secrete reactive oxygen species. The enhanced release of reactive species is known to modify biomolecules like proteins and nucleic acids. Protein carbonylation, one of the most harmful and irreversible protein modifications, is considered as a key player in the progression of diabetes and associated complications. Hence, the present study explores the identification of carbonylated proteins from the monocytes under diabetic stress and determination of their site of modification. Combined avidin affinity chromatography and bottom‐up proteomics experiments identified 13 consistently expressed carbonylated proteins. Most of the identified proteins were reported to have altered functions under diabetic conditions that contribute to the development of diabetes‐associated inflammation and complications. We were able to determine oxidative stress‐induced modifications on Lys, Val, Ile, Cys, Thr and Asp residues.
Pharmacokinetic study of three different formulations of l‐tetrahydropalmatine in brain tissues of rats
Authors: Li Yiran , Zhang Teng , Huai Jiaxin , Cheng Congcong , Xie Linlin , Wang Siqi , Dai Ronghua 
Published: January 2021

Abstractl‐Tetrahydropalmatine (l‐THP), an active alkaloid compound isolated from Rhizoma Corydalis‐yanhusuo, has been reported to possess biological activity for treating cocaine use. To enhance both oral bioavailability and brain penetration, three formulations of l‐THP suspension, mixture of l‐THP–puerarin and self‐microemulsifying drug delivery systems (SMEDDS) were prepared. A sensitive and reliable ultra‐high‐performance liquid chromatography with tandem mass spectrometry method was developed and validated for the simultaneous determination of l‐THP and its active metabolite l‐isocorypalmine (l‐ICP) in rat brain. Diazepam was used as the internal standard. The chromatographic separation was achieved on a Bonshell ASB C18 column at 30°C using acetonitrile–aqueous formic acid as mobile phase in gradient mode. The linearity was validated over the concentration ranges of 4.00–2,500 ng/ml for l‐THP and 0.400–500 ng/ml for l‐ICP. Full method validation was within the acceptance limits. The method was successfully used to determine the pharmacokinetics of two analytes following oral administration of these three formulations to rats. A significant difference was observed in the main pharmacokinetic parameters between SMEDDS and the suspension, and a 3.25‐ and 2.97‐fold increase in the relative bioavailability of l‐THP and l‐ICP, respectively, was observed with the SMEDDS compared with the suspension formulation. It was concluded that SMEDDS enhanced the absorption of l‐THP and l‐ICP and delayed their release in brain.
Simultaneous analysis of drugs administered to lung‐transplanted patients using liquid chromatography–tandem mass spectrometry for therapeutic drug monitoring
Authors: Takasaki Shinya , Hirasawa Tensei , Sato Yu , Maekawa Masamitsu , Tsukamoto Taku , Kikuchi Masafumi , Ogura Jiro , Hayakawa Yoshihiro , Matsuda Yasushi , Oishi Hisashi , Sado Tetsu , Noda Masafumi , Okada Yoshinori , Yamaguchi Hiroaki , Mano Nariyasu 
Published: January 2021

AbstractSeveral drugs are administered to lung‐transplanted patients, which are monitored using therapeutic drug monitoring (TDM). Therefore, we developed and validated a liquid chromatography–tandem mass spectrometry method to simultaneously analyze immunosuppressive drugs such as mycophenolic acid, antifungal drugs such as voriconazole and itraconazole, and its metabolite hydroxyitraconazole. Chromatographic separation was achieved using a C18 column and gradient flow of mobile phase comprising 20 mM aqueous ammonium formate and 20 mM ammonium formate‐methanol solution. A simple protein precipitation treatment was performed using acetonitrile/methanol and mycophenolic acid‐2H3, voriconazole‐2H3, itraconazole‐2H4, and hydroxyitraconazole‐2H4 as internal standards. The linearity ranges of mycophenolic acid, voriconazole, itraconazole, and hydroxyitraconazole were 100–20,000, 50–10,000, 5–1000, and 5–1000 ng/mL, respectively. The retention time of each target was less than 2 min. The relative errors in intra‐ and inter‐day were within ±7.6%, the coefficient of variation was 8.9% or less for quality control low, medium, and high, and it was 15.8% or less for lower limit of quantitation. Moreover, the patient samples were successfully quantified, and they were within the linear range of measurements. Therefore, our new method may be useful for TDM in lung‐transplanted patients.
Metabolomic analysis of plasma from normal‐weight adults with hypo‐HDL cholesterolemia by UPLC–QTOF MS
Authors: Han Pei , Man Jin , Hao Yun , Wu Longjie , Wang Jia , Yang Wenjie , Wang Fudi , Tian Yongmei 
Published: January 2021

AbstractHigh‐density lipoprotein cholesterol (HDL‐C) is negatively correlated with atherosclerotic cardiovascular disease. The prevalence of hypo‐HDL cholesterolemia is as high as 33.9%. The plasma metabolomic differences between hypo‐HDL cholesterolemia populations and normal controls were investigated using ultra‐high‐performance liquid chromatography–quadrupole time‐of‐flight mass spectrometry. Participants with hypo‐HDL cholesterolemia and normal controls were clearly discriminated from each other on the orthogonal partial least squares–discriminant analysis score plot and a total of 90 differential metabolites were identified, including down‐regulated phosphatidylserine [18:0/20:3(8Z,11Z,14Z)], phosphatidylcholine [19:0/18:3(6Z,9Z,12Z)], phosphatidylserine, phosphatidylethanolamine [18:0/20:4(5Z,8Z,11Z,13E) (15Ke)], etc., and up‐regulated triglyceride [15:0/18:1(9Z)/18:3(9Z,12Z,15Z)][iso6], 13‐methyl‐1‐tritriacontene, tridodecylamine, etc. Most of the changed metabolites were lipids, notably, a significant part of which were odd chain fatty acid incorporated lipids. Carnitine shuttle was the most significant metabolic pathway, except for the disturbed glycerophospholipid metabolism, glycosphingolipid metabolism and sphingolipid metabolism in participants with hypo‐HDL cholesterolemia. We identified the key metabolites and metabolic pathways that may be changed in hypo‐HDL cholesterolemia participants, providing useful clues for studying the metabolic mechanisms and for early prevention of hypo‐HDL cholesterolemia and dyslipidemia.
Quality evaluation of rhubarb based on qualitative analysis of the HPLC fingerprint and UFLC–Q‐TOF–MS/MS combined with quantitative analysis of eight anthraquinone glycosides by QAMS
Authors: Dou Zhihua , Dai Ying , Zhou Yunzhong , Wang Shengnan 
Published: January 2021

AbstractRhubarb is one of the most ancient and important herbal medicines, but its current quality evaluation (QE) methods have some limitations. In this study, a new method was developed for the comprehensive QE of rhubarb. First, fingerprints of 28 batches of three species of rhubarb samples were determined by HPLC, the reference fingerprint was established and the common peaks were assigned. Second, the components of common peaks in the fingerprints were identified by ultrafast liquid chromatography quadrupole time‐of‐flight mass spectrometry. Finally, a method for the simultaneous determination of the contents of eight anthraquinone glycosides in rhubarb using quantitative analysis of multiple components by a single marker (QAMS) was established, and the contents of these eight components in 28 batches of rhubarb determined by QAMS and the external standard method were compared. The results showed that there were 31 common peaks in the rhubarb fingerprint. The components of these 31 common peaks were identified, and 20 of them were unambiguously confirmed by reference substances, including eight anthraquinone glycosides. The contents of eight anthraquinone glycosides in the 28 batches of rhubarb determined by QAMS and the external standard method were not significantly different. In conclusion, the method established in this study can be used for the comprehensive QE of rhubarb and can also provide a reference for the QE of other herbal medicines.
A network structure of manic symptoms
Authors: Briganti Giovanni , Kornreich Charles , Linkowski Paul 
Published: January 2021

AbstractObjectivesThe aim of this study is to explore mania as a network of its symptoms, inspired by the network approach to mental disorders.MethodsNetwork structures of both cross‐sectional and temporal effects were measured at three time points (admission, middle of hospital stay, and discharge) in a sample of 100 involuntarily committed patients diagnosed with bipolar I disorder with severe manic features and hospitalized in a specialized psychiatric ward.ResultsElevated mood is the most interconnected symptom in the network on admission, while aggressive behavior and irritability are highly predictive of each other, as well as language‐thought disorder and “content” (the presence of abnormal ideas or delusions). Elevated mood is influenced by many symptoms in the temporal network.ConclusionsThe investigation of manic symptoms with network analysis allows for identifying important symptoms that are better connected to other symptoms at a given moment and over time. The connectivity of the manic symptoms evolves over time. Central symptoms could be considered as targets for clinical intervention when treating severe mania.
Maternal depression and childhood injury risk: A population‐based cohort study in Denmark
Authors: Lyngsøe Bente Kjær , Munk‐Olsen Trine , Vestergaard Claus Høstrup , Rytter Dorte , Christensen Kaj Sparle , Bech Bodil Hammer 
Published: January 2021

AbstractAimsTo assess the association between different stages of maternal depression and injury risk in offspring aged 0–10 years.MethodsPopulation‐based cohort study of all live‐born children in Denmark from 1 January 1997 until 31 December 2013 (n = 1,064,387). Main outcome measure was emergency department contacts with a main diagnosis of injury coded as DS00‐DT98 (chapter XIX) according to the ICD‐10. All information was obtained from Danish national registries.ResultsMaternal depression was associated with higher injury hazard in the offspring throughout childhood compared to offspring of mothers with no history of depression. The strongest association was seen for the first year of life. First‐time maternal depression was most strongly associated with injury in the child, especially in the first year of life (aHR = 1.70, 95% CI: 1.48–1.96). Children of mothers with relapse depression had 1.57 higher hazard of injury in the first year of life (aHR: 1.57, 95% CI: 1.44–1.70). Children of mothers with previously treated depression (postdepression) had 1.13 higher hazard of injury in the first year of life (aHR: 1.13, 95% CI: 1.09–1.17). Continuous treatment for depression was associated with a nonsignificant higher hazard of injuries in the first year of life (aHR: 1.06, 95% CI: 0.91–1.23).ConclusionsMaternal depression was associated with higher injury risk in the offspring, particularly in early childhood. The association persisted in children of mothers with relapse depression. Our results suggest that children of mothers with depression are vulnerable several years after depression onset and treatment cessation.
Components determining the slowness of information processing in parkinson’s disease
Authors: Arroyo Aida , Periáñez José A. , Ríos‐Lago Marcos , Lubrini Genny , Andreo Jorge , Benito‐León Julián , Louis Elan D. , Romero Juan Pablo 
Published: January 2021

AbstractIntroductionBradyphrenia is a key cognitive feature in Parkinson's disease (PD). There is no consensus on whether information processing speed is impaired or not beyond motor performance.ObjectiveThis study aims to explore which perceptual, motor, or cognitive components of information processing are involved in the slowdown affecting cognitive performance.MethodsThe study included 48 patients with PD (age: 63, 3 ± 8, 18; HY I‐III; UPDRS 15,46 ± 7,76) and 53 healthy controls (age: 60,09 ± 12,83). Five reaction time (RT) tasks were administered to all participants. The average RT in each of the tasks and the percentage of correct answers were measured. Patients with PD were in "ON state" at the time of the evaluation. Perceptual, motor, and cognitive components were isolated by means of a series of ANCOVAs.ResultsAs expected, the motor component was slowed down in patients with PD. Moreover, while patients with PD showed slower RT than controls in all tasks, differences between groups did not exponentially increase with the increasing task complexity. ANCOVA analyses also revealed that the perceptual and sustained alert component resulted to be slowed down, with no differences being found in any of the remaining isolated cognitive components (i.e., response strategy‐inhibition, decisional, visual search, or interference control).ConclusionsThe results revealed that slowness of information processing in PD was mainly associated with an impaired processing speed of the motor and perceptual‐alertness components analyzed. The results may help designing new neurorehabilitation strategies, focusing on the improvement of perceptual and alertness mechanisms.
Injection of hybrid 3D spheroids composed of podocytes, mesenchymal stem cells, and vascular endothelial cells into the renal cortex improves kidney function and replenishes glomerular podocytes
Authors: Yang Wen‐Yu , Chen Li‐Chi , Jhuang Ya‐Ting , Lin Yu‐Jie , Hung Pei‐Yu , Ko Yi‐Ching , Tsai Meng‐Yu , Lee Yun‐Wei , Hsu Li‐Wen , Yeh Chih‐Kuang , Hsu Hsiang‐Hao , Huang Chieh‐Cheng 
Published: January 2021

AbstractPodocytes are highly differentiated epithelial cells that are crucial for maintaining the glomerular filtration barrier in the kidney. Podocyte injury followed by depletion is the major cause of pathological progression of kidney diseases. Although cell therapy has been considered a promising alternative approach to kidney transplantation for the treatment of kidney injury, the resultant therapeutic efficacy in terms of improved renal function is limited, possibly owing to significant loss of engrafted cells. Herein, hybrid three‐dimensional (3D) cell spheroids composed of podocytes, mesenchymal stem cells, and vascular endothelial cells were designed to mimic the glomerular microenvironment and as a cell delivery vehicle to replenish the podocyte population by cell transplantation. After creating a native glomerulus‐like condition, the expression of multiple genes encoding growth factors and basement membrane factors that are strongly associated with podocyte maturation and functionality was significantly enhanced. Our in vivo results demonstrated that intrarenal transplantation of podocytes in the form of hybrid 3D cell spheroids improved engraftment efficiency and replenished glomerular podocytes. Moreover, the proteinuria of the experimental mice with hypertensive nephropathy was effectively reduced. These data clearly demonstrated the potential of hybrid 3D cell spheroids for repairing injured kidneys.
Subcellular Localisation of a Quinoline‐Containing Fluorescent Cyclometallated IrIII Complex in Plasmodium falciparum
Authors: Melis Diana R. , Hsiao Chiao‐Yu , Combrinck Jill M. , Wiesner Lubbe , Smith Gregory S. 
Published: January 2021

AbstractA fluorescent analogue of a previously synthesised N,N‐chelated IrIII complex was prepared by coordination of the organic ligand to an extrinsic bis(2‐phenylpyridine)iridium(III) fluorophore. This cyclometallated IrIII complex in itself displays good, micromolar activity against the chloroquine‐sensitive NF54 strain of Plasmodium falciparum. Live‐cell confocal microscopy found negligible localisation of the fluorescent complex within the digestive vacuole of the parasite. This eliminated the haem detoxification pathway as a potential mechanism of action. Similarly, no localisation of the complex within the parasitic nucleus was found, thus suggesting that this complex probably does not interfere with the DNA replication process. A substantial saturation of fluorescence from the complex was found near phospholipid structures such as the plasma and nuclear membranes but not in neutral lipid bodies. This indicates that an association with these membranes, or organelles such as the endoplasmic reticulum or branched mitochondrion, could be essential to the efficacies of these types of antimalarial compounds.
Artificial Organelles: Towards Adding or Restoring Intracellular Activity
Authors: Oerlemans Roy A. J. F. , Timmermans Suzanne B. P. E. , van Hest Jan C. M. 
Published: January 2021

AbstractCompartmentalization is one of the main characteristics that define living systems. Creating a physically separated microenvironment allows nature a better control over biological processes, as is clearly specified by the role of organelles in living cells. Inspired by this phenomenon, researchers have developed a range of different approaches to create artificial organelles: compartments with catalytic activity that add new function to living cells. In this review we will discuss three complementary lines of investigation. First, orthogonal chemistry approaches are discussed, which are based on the incorporation of catalytically active transition metal‐containing nanoparticles in living cells. The second approach involves the use of premade hybrid nanoreactors, which show transient function when taken up by living cells. The third approach utilizes mostly genetic engineering methods to create bio‐based structures that can be ultimately integrated with the cell's genome to make them constitutively active. The current state of the art and the scope and limitations of the field will be highlighted with selected examples from the three approaches.
Small bowel obstruction secondary to primary enterolith: A rare and delayed complication of radiation enteritis
Authors: Seretis Charalampos , Archer Lucy , Elhassan Mohammed Ali , Gurung Deborah , Palit Amitabh , Zayyan Kassim 
Published: January 2021

AbstractPrimary enteroliths as a result of pelvic radiotherapy are a rare cause of intestinal obstruction.
New onset of sinoatrial block in a young patient treated with systemic meglumine antimoniate
Authors: Daadaa Najla , Youssef Soumaya , Haggui Abdeddayem , Harbaoui Sarra , Jaber Kahena , Doss Nejib , Dhaoui Mohamed Raouf 
Published: January 2021

AbstractThe sinoatrial block is a new side effect of meglumine antimoniate. Prompt interruption of the drug results in the normalization of electrographic changes and prevents sudden cardiac arrest.