bluuebunny/crossref_metadata_2025_split huggingface dataset:
Tailward Flows in the Vicinity of Fast Earthward Flows
Authors: Chong Ghai Siung , De Spiegeleer A. , Hamrin M. , Pitkänen T. , Gunell H. , Aizawa S. 
Published: March 2021

AbstractThe occurrence of tailward flows in the magnetotail plasma sheet is closely linked to the dynamics of earthward bursty bulk flows (BBFs). Tailward flows that are observed in the vicinity of these BBFs (or TWABs – Tailward flows around BBFs) may hold unique information on its origin. In this study, we conduct a statistical survey on TWABs by using data from the Cluster mission. We find that TWABs are observed in the vicinity of ∼75% of the BBFs and their occurrence does not depend on BBF velocity magnitude. TWABs have a flow convection pattern consistent with the general tailward flows (GTWs) in the plasma sheet and they do not resemble vortical‐like flows. However, TWABs have a flow velocity magnitude twice larger than the GTWs. The plasma density and temperature of TWABs are comparable with BBFs. It is more common to observe a TWAB succeeding than preceding a BBF. However, there is no distinctive difference (in flow pattern, plasma density and temperature) between preceding and succeeding TWABs. We suggest that TWABs are likely the “freshly” rebounded BBFs from the near‐Earth region where the magnetic field is stronger. TWABs may represent the early stage of the evolution of tailward flows in the plasma sheet. We also discuss and argue that other mechanisms such as shear‐induced vortical flows and tailward slipping of depleted flux tubes cannot be the principal causes of TWABs.
Age‐Depth Stratigraphy of Pine Island Glacier Inferred From Airborne Radar and Ice‐Core Chronology
Authors: Bodart J. A. , Bingham R. G. , Ashmore D. W. , Karlsson N. B. , Hein A. S. , Vaughan D. G. 
Published: March 2021

AbstractUnderstanding the contribution of the West Antarctic Ice Sheet (WAIS) to past and future sea level has been a major scientific priority over the last three decades. In recent years, observed thinning and ice‐flow acceleration of the marine‐based Pine Island Glacier has highlighted that understanding dynamic changes is critical to predicting the long‐term stability of the WAIS. However, relatively little is known about the evolution of the catchment during the Holocene. Internal reflecting horizons (IRHs) provide a cumulative record of accumulation, basal melt, and ice dynamics that, if dated, can be used to constrain ice‐flow models. Here, we use airborne radars to trace four spatially extensive IRHs deposited in the late Quaternary across the Pine Island Glacier catchment. We use the WAIS Divide ice‐core chronology to assign ages to three IRHs: 4.72 ± 0.28, 6.94 ± 0.31, and 16.50 ± 0.79 ka. We use a 1‐D model, constrained by observational and modeled accumulation rates, to produce an independent validation of our ice‐core‐derived ages and provide an age estimate for our shallowest IRH (2.31–2.92 ka). We find that our upper three IRHs correspond to three large peaks in sulfate concentrations in the WAIS Divide ice‐core record and hypothesize that the origin of these spatially extensive IRHs is from past volcanic activity. The clear correspondence between our IRHs and the ones previously identified over the Weddell Sea Sector, altogether representing ∼20% of the WAIS, indicates that a unique set of stratigraphic markers spanning the Holocene exists over a large part of West Antarctica.
The lncRNA Snhg1-Vps13D vesicle trafficking system promotes memory CD8 T cell establishment via regulating the dual effects of IL-7 signaling
Authors: Zhang Yanyan , Li Baohua , Bai Qiang , Wang Pengcheng , Wei Gang , Li Zhirong , Hu Li , Tian Qin , Zhou Jing , Huang Qizhao , Wang Zhiming , Yue Shuai , Wu Jialin , Yang Liuqing , Zhou Xinyuan , Jiang Lubin , Ni Ting , Ye Lilin , Wu Yuzhang 
Published: March 2021

AbstractThe efficient induction and long-term persistence of pathogen-specific memory CD8 T cells are pivotal to rapidly curb the reinfection. Recent studies indicated that long-noncoding RNAs expression is highly cell- and stage-specific during T cell development and differentiation, suggesting their potential roles in T cell programs. However, the key lncRNAs playing crucial roles in memory CD8 T cell establishment remain to be clarified. Through CD8 T cell subsets profiling of lncRNAs, this study found a key lncRNA-Snhg1 with the conserved naivehi-effectorlo-memoryhi expression pattern in CD8 T cells of both mice and human, that can promote memory formation while impeding effector CD8 in acute viral infection. Further, Snhg1 was found interacting with the conserved vesicle trafficking protein Vps13D to promote IL-7Rα membrane location specifically. With the deep mechanism probing, the results show Snhg1-Vps13D regulated IL-7 signaling with its dual effects in memory CD8 generation, which not just because of the sustaining role of STAT5-BCL-2 axis for memory survival, but more through the STAT3-TCF1-Blimp1 axis for transcriptional launch program of memory differentiation. Moreover, we performed further study with finding a similar high-low-high expression pattern of human SNHG1/VPS13D/IL7R/TCF7 in CD8 T cell subsets from PBMC samples of the convalescent COVID-19 patients. The central role of Snhg1-Vps13D-IL-7R-TCF1 axis in memory CD8 establishment makes it a potential target for improving the vaccination effects to control the ongoing pandemic.
FGF1ΔHBS prevents diabetic cardiomyopathy by maintaining mitochondrial homeostasis and reducing oxidative stress via AMPK/Nur77 suppression
Authors: Wang Dezhong , Yin Yuan , Wang Shuyi , Zhao Tianyang , Gong Fanghua , Zhao Yushuo , Wang Beibei , Huang Yuli , Cheng Zizhao , Zhu Guanghui , Wang Zengshou , Wang Yang , Ren Jun , Liang Guang , Li Xiaokun , Huang Zhifeng 
Published: March 2021

AbstractAs a classically known mitogen, fibroblast growth factor 1 (FGF1) has been found to exert other pleiotropic functions such as metabolic regulation and myocardial protection. Here, we show that serum levels of FGF1 were decreased and positively correlated with fraction shortening in diabetic cardiomyopathy (DCM) patients, indicating that FGF1 is a potential therapeutic target for DCM. We found that treatment with a FGF1 variant (FGF1∆HBS) with reduced proliferative potency prevented diabetes-induced cardiac injury and remodeling and restored cardiac function. RNA-Seq results obtained from the cardiac tissues of db/db mice showed significant increase in the expression levels of anti-oxidative genes and decrease of Nur77 by FGF1∆HBS treatment. Both in vivo and in vitro studies indicate that FGF1∆HBS exerted these beneficial effects by markedly reducing mitochondrial fragmentation, reactive oxygen species (ROS) generation and cytochrome c leakage and enhancing mitochondrial respiration rate and β-oxidation in a 5’ AMP-activated protein kinase (AMPK)/Nur77-dependent manner, all of which were not observed in the AMPK null mice. The favorable metabolic activity and reduced proliferative properties of FGF1∆HBS testify to its promising potential for use in the treatment of DCM and other metabolic disorders.
Dental periodontal procedures: a systematic review of contamination (splatter, droplets and aerosol) in relation to COVID-19
Authors: Johnson Ilona G , Jones Rhiannon J , Gallagher Jennifer E. , Wade William G. , Al-Yaseen Waraf , Robertson Mark , McGregor Scott , K. C Sukriti , Innes Nicola , Harris Rebecca 
Published: March 2021

Abstract
Introduction
The emergence of the SARS-CoV-2 virus and subsequent COVID-19 pandemic has had a significant effect on the delivery of routine dentistry; and in particular, periodontal care across the world. This systematic review examines the literature relating to splatter, droplet settle and aerosol for periodontal procedures and forms part of a wider body of research to understand the risk of contamination in relation to periodontal care procedures relevant to COVID-19.

Methods
A search of the literature was carried out using key terms and MeSH words relating to the review questions. Sources included Medline (OVID), Embase (OVID), Cochrane Central Register of Controlled Trials, Scopus, Web of Science and LILACS, ClinicalTrials.Gov. Studies meeting inclusion criteria were screened in duplicate and data extraction was carried out using a template. All studies were assessed for methodological quality and sensitivity. Narrative synthesis was undertaken.

Results
Fifty studies were included in the review with procedures including ultrasonic scaling (n = 44), air polishing (n = 4), prophylaxis (n = 2) and hand scaling (n = 3). Outcomes included bacterial (colony-forming units e.g. on settle plates) or blood contamination (e.g. visible splatter) and non bacterial, non blood (e.g. chemiluminescence or coloured dyes) contamination. All studies found contamination at all sites although the contamination associated with hand scaling was very low. Contamination was identified in all of the studies even where suction was used at baseline. Higher power settings created greater contamination. Distribution of contamination varied in relation to operator position and was found on the operator, patient and assistant with higher levels around the head of the operator and the mouth and chest of the patient. Settle was identified 30 min after treatments had finished but returned to background levels when measured at or after an hour. The evidence was generally low to medium quality and likely to underestimate contamination.

Conclusion
Ultrasonic scaling, air polishing and prophylaxis procedures produce contamination (splatter, droplets and aerosol) in the presence of suction, with a small amount of evidence showing droplets taking between 30 min and 1 h to settle. Consideration should be given to infection control, areas of cleaning particularly around the patient and appropriate personal protective equipment, with particular attention to respiratory, facial and body protection for these procedures. In addition, the use of lower power settings should be considered to reduce the amount and spread of contamination.
Nondestructive detection of photonic qubits
Authors: Niemietz Dominik , Farrera Pau , Langenfeld Stefan , Rempe Gerhard 
Published: March 2021

AbstractOne of the biggest challenges in experimental quantum information is to sustain the fragile superposition state of a qubit1. Long lifetimes can be achieved for material qubit carriers as memories2, at least in principle, but not for propagating photons that are rapidly lost by absorption, diffraction or scattering3. The loss problem can be mitigated with a nondestructive photonic qubit detector that heralds the photon without destroying the encoded qubit. Such a detector is envisioned to facilitate protocols in which distributed tasks depend on the successful dissemination of photonic qubits4,5, improve loss-sensitive qubit measurements6,7 and enable certain quantum key distribution attacks8. Here we demonstrate such a detector based on a single atom in two crossed fibre-based optical resonators, one for qubit-insensitive atom–photon coupling and the other for atomic-state detection9. We achieve a nondestructive detection efficiency upon qubit survival of 79 ± 3 per cent and a photon survival probability of 31 ± 1 per cent, and we preserve the qubit information with a fidelity of 96.2 ± 0.3 per cent. To illustrate the potential of our detector, we show that it can, with the current parameters, improve the rate and fidelity of long-distance entanglement and quantum state distribution compared to previous methods, provide resource optimization via qubit amplification and enable detection-loophole-free Bell tests.
Streptococcus agalactiae amylomaltase offers insight into the transglycosylation mechanism and the molecular basis of thermostability among amylomaltases
Authors: Tumhom Suthipapun , Nimpiboon Pitchanan , Wangkanont Kittikhun , Pongsawasdi Piamsook 
Published: March 2021

AbstractAmylomaltase (AM) catalyzes transglycosylation of starch to form linear or cyclic oligosaccharides with potential applications in biotechnology and industry. In the present work, a novel AM from the mesophilic bacterium Streptococcus agalactiae (SaAM), with 18–49% sequence identity to previously reported AMs, was characterized. Cyclization and disproportionation activities were observed with the optimum temperature of 30 °C and 40 °C, respectively. Structural determination of SaAM, the first crystal structure of small AMs from the mesophiles, revealed a glycosyl-enzyme intermediate derived from acarbose and a second acarbose molecule attacking the intermediate. This pre-transglycosylation conformation has never been before observed in AMs. Structural analysis suggests that thermostability in AMs might be mainly caused by an increase in salt bridges since SaAM has a lower number of salt bridges compared with AMs from the thermophiles. Increase in thermostability by mutation was performed. C446 was substituted with A/S/P. C446A showed higher activities and higher kcat/Km values for starch in comparison to the WT enzyme. C446S exhibited a 5 °C increase in optimum temperature and the threefold increase in half-life time at 45 °C, most likely resulting from H-bonding interactions. For all enzymes, the main large-ring cyclodextrin (LR-CD) products were CD24-CD26 with CD22 as the smallest. C446S produced more CD35-CD42, especially at a longer incubation time.
Cardio-pathogenic variants in unexplained intrauterine fetal death: a retrospective pilot study
Authors: Muin Dana A. , Kollmann Martina , Blatterer Jasmin , Hoermann Gregor , Husslein Peter W. , Lafer Ingrid , Petek Erwin , Schwarzbraun Thomas 
Published: March 2021

AbstractTo describe the prevalence and spectrum of cardio-pathogenic variants in singleton fetuses after unexplained intrauterine fetal death (IUFD). DNA from post-mortem fibroblastic tissue samples of 16 fetuses after unexplained IUFD was retrieved at two tertiary university hospitals for clinical exome sequencing with subsequent filtering of 122 cardio-specific genes to elucidate underlying cardio-pathogenic variants. In total, we included 12 (75%) male and four (25%) female fetuses who were stillborn at a median gestational age of 34+6 (23+2–40+5) weeks. In two (12.5%) fetuses no cardio-pathogenic variants were found. In 14 (87.5%) fetuses, overall 33 variants were detected in 22 cardio-specific genes, involving 14 (63.63%) genes associated with cardiomyopathy, six (27.27%) arrhythmogenic susceptibility genes and two (9.09%) arrhythmia and cardiomyopathy associated genes. Among the 33 variants, five (15.2%) were classified as likely benign according to the American College of Medical Genetics and Genomics; 28 (84.8%) variants were considered as variants of uncertain significance. Compared to a cohort of explained IUFDs, the cases with and without fetal variants in cardiac genes differed not significantly regarding maternal age, previous history of stillbirth, time of stillbirth or fetal sex. Unexplained stillbirth may be caused by cardio-genetic pathologies, yet a high number of variants of uncertain significance merit a more detailed post-mortem examination including family segregation analysis.
Investigating heterogeneities of live mesenchymal stromal cells using AI-based label-free imaging
Authors: Imboden Sara , Liu Xuanqing , Lee Brandon S. , Payne Marie C. , Hsieh Cho-Jui , Lin Neil Y. C. 
Published: March 2021

AbstractMesenchymal stromal cells (MSCs) are multipotent cells that have great potential for regenerative medicine, tissue repair, and immunotherapy. Unfortunately, the outcomes of MSC-based research and therapies can be highly inconsistent and difficult to reproduce, largely due to the inherently significant heterogeneity in MSCs, which has not been well investigated. To quantify cell heterogeneity, a standard approach is to measure marker expression on the protein level via immunochemistry assays. Performing such measurements non-invasively and at scale has remained challenging as conventional methods such as flow cytometry and immunofluorescence microscopy typically require cell fixation and laborious sample preparation. Here, we developed an artificial intelligence (AI)-based method that converts transmitted light microscopy images of MSCs into quantitative measurements of protein expression levels. By training a U-Net+ conditional generative adversarial network (cGAN) model that accurately (mean $$r_s$$

r
s

= 0.77) predicts expression of 8 MSC-specific markers, we showed that expression of surface markers provides a heterogeneity characterization that is complementary to conventional cell-level morphological analyses. Using this label-free imaging method, we also observed a multi-marker temporal-spatial fluctuation of protein distributions in live MSCs. These demonstrations suggest that our AI-based microscopy can be utilized to perform quantitative, non-invasive, single-cell, and multi-marker characterizations of heterogeneous live MSC culture. Our method provides a foundational step toward the instant integrative assessment of MSC properties, which is critical for high-throughput screening and quality control in cellular therapies.
Microdroplet-based one-step RT-PCR for ultrahigh throughput single-cell multiplex gene expression analysis and rare cell detection
Authors: Ma Jennifer , Tran Gary , Wan Alwin M. D. , Young Edmond W. K. , Kumacheva Eugenia , Iscove Norman N. , Zandstra Peter W. 
Published: March 2021

AbstractGene expression analysis of individual cells enables characterization of heterogeneous and rare cell populations, yet widespread implementation of existing single-cell gene analysis techniques has been hindered due to limitations in scale, ease, and cost. Here, we present a novel microdroplet-based, one-step reverse-transcriptase polymerase chain reaction (RT-PCR) platform and demonstrate the detection of three targets simultaneously in over 100,000 single cells in a single experiment with a rapid read-out. Our customized reagent cocktail incorporates the bacteriophage T7 gene 2.5 protein to overcome cell lysate-mediated inhibition and allows for one-step RT-PCR of single cells encapsulated in nanoliter droplets. Fluorescent signals indicative of gene expressions are analyzed using a probabilistic deconvolution method to account for ambient RNA and cell doublets and produce single-cell gene signature profiles, as well as predict cell frequencies within heterogeneous samples. We also developed a simulation model to guide experimental design and optimize the accuracy and precision of the assay. Using mixtures of in vitro transcripts and murine cell lines, we demonstrated the detection of single RNA molecules and rare cell populations at a frequency of 0.1%. This low cost, sensitive, and adaptable technique will provide an accessible platform for high throughput single-cell analysis and enable a wide range of research and clinical applications.
Controlling T cells spreading, mechanics and activation by micropatterning
Authors: Sadoun Anaïs , Biarnes-Pelicot Martine , Ghesquiere-Dierickx Laura , Wu Ambroise , Théodoly Olivier , Limozin Laurent , Hamon Yannick , Puech Pierre-Henri 
Published: March 2021

AbstractWe designed a strategy, based on a careful examination of the activation capabilities of proteins and antibodies used as substrates for adhering T cells, coupled to protein microstamping to control at the same time the position, shape, spreading, mechanics and activation state of T cells. Once adhered on patterns, we examined the capacities of T cells to be activated with soluble anti CD3, in comparison to T cells adhered to a continuously decorated substrate with the same density of ligands. We show that, in our hand, adhering onto an anti CD45 antibody decorated surface was not affecting T cell calcium fluxes, even adhered on variable size micro-patterns. Aside, we analyzed the T cell mechanics, when spread on pattern or not, using Atomic Force Microscopy indentation. By expressing MEGF10 as a non immune adhesion receptor in T cells we measured the very same spreading area on PLL substrates and Young modulus than non modified cells, immobilized on anti CD45 antibodies, while retaining similar activation capabilities using soluble anti CD3 antibodies or through model APC contacts. We propose that our system is a way to test activation or anergy of T cells with defined adhesion and mechanical characteristics, and may allow to dissect fine details of these mechanisms since it allows to observe homogenized populations in standardized T cell activation assays.
Effects of prolonged type 2 diabetes on changes in peripapillary retinal nerve fiber layer thickness in diabetic eyes without clinical diabetic retinopathy
Authors: Lee Min-Woo , Lim Hyung-Bin , Kim Min-Su , Park Gi-Seok , Nam Ki-Yup , Lee Young-Hoon , Kim Jung-Yeul 
Published: March 2021

AbstractTo identify the effects of prolonged type 2 diabetes (T2DM) on changes in peripapillary retinal nerve fiber layer (pRNFL) thickness in patients without clinical diabetic retinopathy. Subjects were divided into two groups: controls and patients with T2DM (DM group). After the initial visits, the pRNFL thicknesses were measured three more times at 1-year intervals. Subgroup analyses were performed in patients with T2DM duration ≥ 10 years. The mean pRNFL thickness at each visit was 95.8 ± 8.1, 95.4 ± 8.3, 94.9 ± 8.1, and 94.5 ± 8.3 μm in the control group (P = 0.138) (n = 55); and 93.4 ± 9.1, 92.1 ± 9.3, 90.9 ± 9.3, and 89.5 ± 9.2 μm in the DM group (P < 0.001) (n = 85). The estimated rate of reduction in mean pRNFL thickness was − 0.45 μm/year in the control group and − 1.34 μm/year in the DM group, respectively. In the DM group, the BCVA and HbA1c (bothP = 0.001) were significant factors associated with pRNFL reduction. In patients with T2DM duration ≥ 10 years, the estimated pRNFL reduction rate was − 1.61 μm/year, and hypertension was a significant factor affecting the pRNFL reduction (P = 0.046). We confirmed rapid pRNFL reduction over time in T2DM, and the reduction rate was higher in patients with T2DM ≥ 10 years. Additionally, BCVA and HbA1c levels were significantly associated with the change in pRNFL thickness in T2DM patients.
Common variants in KCNK5 and FHL5 genes contributed to the susceptibility of migraine without aura in Han Chinese population
Authors: Jiang Zhao , Zhao Longrui , Zhang Xiaojie , Zhang Wenjuan , Feng Yuxing , Li Tao 
Published: March 2021

AbstractA recent genome-wide meta study suggested that rs67338227 in the FHL5 gene and rs10456100 in the KCNK5 gene are associated with migraine from 27 population-based cohorts excluding Chinese population. Given that migraine without aura (MO) is the most common subtype of migraine, our aim was to systematically investigate the relationship of common variants in FHL5 and KCNK5 genes with the susceptibility to MO and provide clues as to the nature of the mechanisms involved in the etiology of migraine. A total of 3306 subjects including 1042 patients with MO and 2264 controls were recruited for the discovery stage, and 2530 individuals including 842 patients with MO and 1688 controls for the replication stage. Twenty-two tag SNPs (7 from FHL5 and 15 from KCNK5) were selected for genotyping. Genetic associations were analyzed at both single-marker and haplotype levels. Potential functional consequences of the significant SNPs were analyzed using gene expression data obtained from the GTEx database. Two SNPs, rs10456100 (KCNK5, P = 9.01 × 10–9) and rs7775721 (FHL5, P = 6.86 × 10–13), were determined to be significantly associated with MO in the discovery sample and were then replicated in another sample. In the combined sample set, the T allele of both SNPs was significantly associated with the increased risk of MO. Significant eQTL signals were identified for both SNP rs10456100 and rs7775721. Our findings suggest that the T allele carriers of SNP rs10456100 and rs7775721 are at increased risk of migraine.