bluuebunny/crossref_metadata_2025_split huggingface dataset:
Decoding Antibacterial and Antibiofilm Properties of Cinnamon and Cardamom Essential Oils: A Combined Molecular Docking and Experimental Study
Authors: Pourkhosravani Elahe , nayeri fatemeh dehghan , Bazargani Mitra Mohammadi 
Published: October 2021

Abstract
This study sets out to compare the antibacterial and antibiofilm profiles of Ci/Ca EOs alone and in combination together against infectious bacterial strains. MIC assay was carried out to survey the effectiveness of prepared EOs by two-fold serial dilution method and MTT evaluation. Synergic antibacterial properties of EOs against target strains were studied by using checkerboard titration method. Biofilm growth and development were evaluated using CV and XTT reduction assays. Antibacterial activity was observed for EOs against both bacterial strains with stronger activity for CiEO against both bacteria. The synergistic antibacterial effect was observed only against B. subtilis. Based on the FIC index, combinations could not inhibit the growth of E. coli. The pure EOs and their combination inhibited cell attachment for both studied bacteria with stronger effect on E. coli. CV and XTT reduction assays results showed that Ci EO and its combination with CaEO had the highest antibiofilm activity at lowest MIC value 0.08% and 0.04/0.02% against biofilm formed by E. coli and B. subtilis respectively, indicating a high antibiofilm potential. Computational docking analyses also postulated that the active constituents of evaluated EOs have the potential to interact with different bacterial targets, suggested binding mode of action of EOs metabolites. By and large, synergistic anti-biofilm properties of EOs may provide further options for developing novel formula to inhibit a variety of infectious clinical and industrial strains without (or less) toxicity effects on human body.
Monitoring Alpha-Synuclein – Tau Interactions In Vitro and In Vivo Using Bimolecular Fluorescence Complementation
Authors: Torres-Garcia Laura , Domingues Joana M.P. , Brandi Edoardo , Haikal Caroline , Brás Inês C. , Gerhardt Ellen , Li Wen , Svanbergsson Alexander , Outeiro Tiago F. , Gouras Gunnar K. , Li Jia-Yi 
Published: October 2021

Abstract
Parkinson’s disease (PD) and Alzheimer’s disease (AD) are characterized by pathological accumulation and aggregation of different amyloidogenic proteins, α-synuclein (aSyn) in PD, and amyloid-β (Aβ) and Tau in AD. Strikingly, few PD and AD patients’ brains exhibit pure pathology with most cases presenting mixed types of protein deposits in the brain. Bimolecular fluorescence complementation (BiFC) is a technique based on the complementation of two halves of a fluorescent protein, which allows direct visualization of protein-protein interactions. In the present study, we assessed the ability of aSyn and Tau to interact with each other. For in vitro evaluation, HEK293 and human neuroblastoma cells were used, while in vivo studies were performed by AAV6 injection in the substantia nigra pars compacta (SNpc) of mice and rats. We observed that the co-expression of aSyn and Tau led to the emergence of fluorescence, reflecting the interaction of the proteins in cell lines, as well as in mouse and rat SNpc. Thus, our data indicates that aSyn and Tau are able to interact with each other in a biologically relevant context, and that the BiFC assay is an effective tool for studying aSyn-Tau interactions in vitro and in different rodent models in vivo.
Cooperative Cathodes for Enhanced Hexavalent Chromium Reduction and Electricity Generation in Bioelectrochemical Reactor with Simultaneous Sludge Degradation
Authors: Yu Hang , Zhao Qingliang , Cao Yuhui , Sun Tiantian , Liang Zhuyuan , Liu Weifeng , Zhu Yimin 
Published: October 2021

Abstract
In a typical microbial electrochemical system (MES) with dual-chamber for Cr(VI) reduction, it was faced with the decline of Cr(VI) reduction efficiency after several cycles of operation, which limited the continuous and effective Cr(VI) reduction of MES. In this study, a novel bioelectrochemical reactor assembled with cooperative cathodes of chemical cathode and bio-cathode (BERCC) and with excess sludge as anodic substrate was designed to solve the problem. Comparative study of BERCC and BER with dual chemical cathodes (BERDC) revealed that BERCC broke through the limitation of Cr(VI) reduction decline for cycles of operation and improved performance of electricity generation. Both the Cr(VI) reduction rate and the power density were increased with the decrease of pH and the increase of TCOD and initial Cr(VI) concentration. Cooperative cathodes stimulated the growth of electrochemically active microorganisms in the anodic biofilm and produced 8.21±0.64 mg Coulomb/(L·h) more electrons than dual chemical cathodes, which enhanced the electrons for electricity generation and Cr(VI) reduction by about 58.3% and 56.1±5.6% in BERCC than those in BERDC.
Caveolae respond to acute oxidative stress through membrane lipid peroxidation, cytosolic release of CAVIN1, and downstream regulation of NRF2
Authors: Wu Yeping , Lim Ye-Wheen , Stroud David , Martel Nick , Hall Thomas , Lo Harriet , Ferguson Charles , Ryan Michael , McMahon Kerrie-Ann , Parton Robert 
Published: October 2021

Abstract
Caveolae have been linked to many biological functions, but their precise roles are unclear. Using quantitative whole cell proteomics of genome-edited cells, we show that the oxidative stress response is the major pathway dysregulated in cells lacking the key caveola structural protein, CAVIN1. CAVIN1 deletion compromised sensitivity to oxidative stress in cultured cells and in animals. Wound-induced accumulation of reactive oxygen species and apoptosis were suppressed in Cavin1-null zebrafish, negatively affecting regeneration. Oxidative stress triggered lipid peroxidation (LPO) and induced caveolar disassembly. The resulting release of CAVIN1 from caveolae allowed direct interaction between CAVIN1 and NRF2, a key regulator of the antioxidant response, facilitating NRF2 degradation. CAVIN1-null cells with impaired negative regulation of NRF2 showed resistance to LPO-induced ferroptosis. Thus, caveolae, via LPO and CAVIN1 release, maintain cellular susceptibility to oxidative stress-induced cell death demonstrating a crucial role for this enigmatic organelle in cellular homeostasis and wound response.
A Ferroptosis-Related Long Non-Coding RNA Signature Predicts Prognosis and Immune Microenvironment for Lung Squamous Cell Carcinoma
Authors: Liu Zhiyang , Xie Zhongcheng , Zhi Chenxi , Lin Xiaoyan , Ma Wentao , Tan Xiaoqian , Li Pin , Ouyang Siyu , Yu Jiang , Chen Xi , Peng Tianhong , Li Liang , Xie Wei 
Published: October 2021

Abstract
Background: Ferroptosis-related lncRNAs (FerLncRNAs) were developed to play a significant role in cancer treatment and prognosis. However, the relationship between FerLncRNAs and Lung squamous cell carcinoma (LUSC) remains unclear.Method: Based on ferroptosis-related differentially expressed lncRNAs in LUSC, we established a prognostic 8-lncRNA signature.Results: 8 Ferroptosis-related lncRNAs (LUCAT1, AL161431.1, AL122125.1, AC104248.1, AC016924.1, MIR3945HG, C10orf55 and AP006545.2) had prognostic value for LUSC by multivariate COX analysis (P<0.05), and possessed significant association with patient outcomes. Kaplan–Meier curves showed an obvious difference in OS that the high-risk group patients exhibited poorer survival than the low-risk group patients. The clinical receiver operating characteristic (ROC) curve and decision curve analysis (DCA) revealed that the ferroptosis-related lncRNAs prognostic signature can (FerRLSig) emerged more outstanding performance than clinical features in predicting the prognosis of LUSC. GSEA revealed that the majority of the novel Ferroptosis-related lncRNAs signature-regulated immune responses and the immune system processes were enriched in the high-risk group. 34 immune checkpoints (ICs) were detected significantly different expression between high-risk and low-risk groups.Conclusion: A novel FerRLSig based on 8 Ferroptosis-related lncRNAs provided important prognostic value for LUSC patients and developed new insights about ferroptosis-related immunotherapy targets in clinic.
Inhibition of ATR prevents macropinocytosis driven retraction of neurites and opposes invasion in GBM
Authors: Birch Joanna , Strathdee karen , Stevenson Katrina , Derby Sarah , Dutton Louise , Clough Emily , Koessinger Anna , Gilmour Lesley , McGhee Ewan , McGarrity-Cottrell Connor , Dibekeme Aurelie anderlinden , Collis Spencer , Rominiyi Ola , Soares Leandro Lembruber , Solecki Gergely , Winkler Frank , Carlin Leo , Inman Gareth , Chalmers Anthony , Norman Jim , Carruthers Ross 
Published: October 2021

Abstract
Glioblastoma (GBM) is the most common and aggressive type of primary brain tumour and remains incurable despite decades of research. GBM are characterised by highly infiltrative growth patterns that contribute to the profound cognitive and neurological symptoms experienced by patients, and to inevitable recurrence following treatment. Novel treatments that reduce infiltration of the healthy brain have potential to ameliorate clinical symptoms and improve survival. Here, we report a novel role of the Ataxia telangiectasia and Rad 3 related kinase (ATR) in supporting the invasive properties of GBM cells through the regulation of macropinocytosis-driven internalisation of integrin adhesion receptors. We demonstrate that inhibition of ATR opposes GBM migration in vitro, and correspondingly reduces infiltrative behaviour in orthotopic mouse models. These results indicate that ATR inhibition, in addition to its use as a radiosensitiser, may be effective in reducing GBM infiltration and its associated symptoms.
Promoting Cultural Change Towards Dementia Friendly Communities: A Multi-level Intervention in Japan
Authors: Tsuda Shuji , Inagaki Hiroki , Okamura Tsuyoshi , Sugiyama Mika , Ogawa Madoka , Miyamae Fumiko , Edahiro Ayako , Ura Chiaki , Sakuma Naoko , Awata Shuichi 
Published: October 2021

Abstract
BackgroundEffective strategies to develop dementia-friendly communities (DFCs) are needed in aging societies. We aimed to propose a strategy to develop DFCs from a Japanese perspective and to evaluate an intervention program that adopted the strategy.MethodsThis study implemented a multi-level intervention that emphasized nurturing community social capital in a large apartment complex in the Tokyo metropolitan area in 2017. We offered an inclusive café that was open for extended hours as a place to socialize and a center for activities that included monthly public lectures. Individual consultation on daily life issues was also available for free at the café. Postal surveys were sent out to all older residents aged 70 years and older in 2016 and 2019. With a one-group pre-test and post-test design, we assessed changes in the proportion of older residents who had social interaction with friends and those who were confident about living in the community, even if they were living with dementia.ResultsTotals of 2,633 and 2,696 residents completed the pre and post-intervention surveys, respectively. The mean age of the pre-intervention respondents was 77.4 years; 45.7% lived alone and 7.7% reported living with impaired cognitive function. The proportion of men who had regular social interaction and were confident about living in their community with dementia increased significantly from 38.8–44.5% (p=0.008) and from 34.1–38.3% (p=0.045), respectively. Similar significant increases were observed in the subgroup of men living with impaired cognitive function, but not in the same subgroup for women.ConclusionsThe intervention benefitted male residents who were less likely to be involved in the community’s web of social networks at baseline. A strategy to create DFCs that emphasizes nurturing community social capital can form a foundation for DFCs.Trial registrationThis study was retrospectively registered in the University hospital Medical Information Network (UMIN) Clinical Trial Registry (registry number: UMIN000038193, date of registration: Oct 3, 2019).
Preoperative Differentiation of Pancreatic Cystic Neoplasm Subtypes on CT-Based Radiomics: SCN, MCN And IPMN
Authors: Xu Shanshan , Zhang Yifan , Wu Jin , Tang Shengnan , He Jian 
Published: October 2021

Abstract
Background:The serous cystic neoplasm (SCN), mucinous cystic neoplasm (MCN), intraductal papillary mucinous neoplasm (IPMN) comprise the large proportion of pancreatic cystic neoplasm (PCN). The appropriate clinical management of MCN and IPMN isextremely essential to improve the 5-years survival rate for the early detection of pancreatic cancer. However, the differential diagnosis of patients with PCN before the treatment is still a tough challenge for all surgeons. Therefore, a reliable diagnosis tool is urgently required to be established for the improvement of precision diagnostics.Method:Between February 2016 and December 2020, 143 consecutive patients with PCN who were confirmed by postoperative pathology were retrospectively included in the study cohort, randomized into development and test cohort at the ratio of 7:3. The predictors of preoperative clinical-radiologic paraments were evaluated by the use of univariate and multivariable logistic regression analysis. A total of 1218 radiomics features were computationally extracted from the enhanced computed tomography (CT) of tumor region and a radiomics signature was established by the random forest algorithm. In the development cohort, the multi-class and binary-class radiomics models integrating preoperative variables and radiomics features were constructed to distinguish between the three types of PCN. The independent internal test cohort was applied to validate the classification models.Result:All preoperative prediction models were built by integrating the radiomics signature with thirteen diagnosis-related radiomics features and three important clinical-radiologic parameters of age, sex and tumor diameter. The multi-class prediction model presented an overall accuracy of 0.804 in the development cohort and 0.707 in the test cohort. The binary-class prediction models displayed the higher overall accuracy of 0.853, 0.866, 0.928 in the development dataset and 0.750, 0.839, 0.889 in the test dataset. In the test cohort, the binary-class radiomics models showed better predictive performances (AUC = 0.914, 0.863 ,0.926) than the multi-class radiomics model (AUC = 0.850), with a large net benefit in the decisive curve analysis. The radiomics-based nomogram provided the correct predicted probability for the diagnosis of PCN.Conclusion: The proposed radiomics models with clinical-radiologic parameters and radiomics features helped predict the accurate diagnosis among SCN, MCN, and IPMN to advance personalized medicine.
The Ultra-low Frequency and Broad Band Gaps Characteristics of Multilayer Composite Cylindrical Three-dimensional pentamode metamaterials
Authors: Cai Chengxin , Wang Xue , Wang Qifu , Li Mingxing , He Guangchen , Wang Zhaohong , Yao Qin 
Published: October 2021

Abstract
For three-dimensional pentamode metamaterials, it is of great significance to realize underwater ultra-low frequency acoustic wave control. Therefore, two types multilayer composite cylindrical three-dimensional pentamode metamaterials with ultra-low frequency and broad band gaps are proposed in this paper. By using pentamode metamaterials with lattice constants on the order of centimeters, the phononic band gaps below 60 Hz and the single-mode area below 30Hz can be obtained. Compared with asymmetrical double-cone locally resonant pentamode metamaterials, the lower edge frequency, relative bandwidth and figure of merit of the first phononic band gap can be reduced by up to 61.4%, 10.3% and 40.6%, respectively. It will provide reference and guidance for the engineering application of pentamode metamaterials in controlling the ultra-low frequency broadband acoustic waves, vibration and noise reduction.
Mandatory CME Grand Rounds Evaluations: More Data, Less Information
Authors: Wecksell Matthew , Salik Irim 
Published: October 2021

Abstract
Background: For several years, learners have been required to evaluate a continuing medical education (CME) session prior to receiving a certificate of participation from an accredited provider. The mandatory nature of these evaluations has led to a high number of evaluations that offer information of questionable utility. Results: We obtained time stamped evaluation data from our CME services vendor, and compared the times that sessions were evaluated to the start and completion times of those CME sessions. While almost all attendees completed evaluations, 8% did so prior to the start of the session and half did so prior to its completion. Conclusions: Making evaluations mandatory has had the effect of lowering the quality of the data thus obtained. In an age that has been described as the “graying of grand rounds,” there are more effective strategies to enhance educational value and learner satisfaction.
Multimodal Optimization with the Local Optimum Ranking 2 Algorithm
Authors: Duarte Francisco Daniel Filip 
Published: October 2021

Abstract
In optimization tasks, it is interesting to achieve a set of efficient solutions instead of one single output, in the case the best solution is not suitable. Many niching methods offer a diversified response, yet some important problems are common: (1) The most interesting solutions of each local optimum are not identified. Thus, the output is the overall population of solutions, which increases the work of the designer in verifying which solution is the most interesting. (2) Existing niching algorithms tend to distribute the solutions on the most promising regions, over-populating some local optima and sub-populating others, what leads to a poor optimization.To solve these challenges, a novel niching method is presented, named local optimum ranking 2. This sorting methodology favors the exploration of a defined number of local optima, and ranks each local population by objective value within each local optimum. Thus, is performed a multi-focus exploration, with an equalized number of solutions on each local optimum, while identifying which solutions are the local apices. Experimental results demonstrate the local optimum ranking 2 provides superior performance than other popular niching methods, for the selected test functions and global optimization algorithms. Also, its versatility is demonstrated in the several ways it can be combined with some of the most well-known methods.In a second experiment, the LOR2 algorithm is applied in the design optimization of a metallic cantilever beam. It is exemplified how the LOR2 algorithm can achieve a set of efficient and diverse design configurations, identifying which are the apices of each local optimum. Thus, the LOR2 facilitates multimodal optimization tasks, while offering both performance and diversity for design challenges.In addition, a third experiment describes how the algorithm can be applied to segment the domain of any function, with any type of input distribution or number of coordinates, into a mesh of similar sized or custom sized elements. Thus, it can segment a response surface named Kriging, significantly simplifying it and reducing computation time.
“Oral Health-related Quality of Life and Periodontal Status in Chronic Kidney Disease Patients: A Cross Sectional Study”
Authors: Tabesh Adel , Narimany Reyhane , Abtahi Maryam , Fakheran Omid 
Published: October 2021

Abstract
Background: Oral health-related quality of life includes the effect of factors such as oral health and oral function on people’s lifestyles. Chronic kidney disease is caused by the deterioration of kidney structures, decreasing their function. CKD causes some oral problems, and these problems could affect people’s quality of life. This study was conducted to determine OHRQoL and periodontal health status in CKD patients. Methods: One hundred and four CKD patients participated in the study. Their OHRQoL was assessed by Oral Health Impact Profile-14 (OHIP-14), and their periodontal status was assessed by the community periodontal index of treatment needs (CPITN). The stage of the disease was measured by BUN and creatinine. We used t-test, ANOVA, and Pearson’s and Spearman’s correlation tests for statistical analyses. Results: The most frequent CKD stage was 5. The average total score of OHIP-14 in these patients was 14.82 (±4.86). The most frequent CPITN score was 2 (calculus). CPITN had a direct relationship with the total score of OHRQOL (P=0.004) and its physical domains (P<0.05). Conclusion: There was a significant relationship between periodontal status and OHRQoL in CKD patients. The quality of life in this group of patients might be improved by providing their periodontal treatment needs.
An Adaptive Iterative Symbol Clipping Scheme For OFDM-Based Visible Light Communication
Authors: Jia Kejun , Qin Cuicui , Yang Boran , Cao Minghua , Li Suoping 
Published: October 2021

Abstract
In this paper, an adaptive iterative symbol clipping scheme for OFDM-based visible light communication systems is proposed. This scheme can avoid the disadvantages of the conventional iterative signal clipping (ISC) scheme that produces all-zero symbols due to the fixed iterative clipping times, and also avoids the information transmission rate degradation due to an adaptive symbol decomposition with serial transmission (ASDST) scheme using serial transmission. In the proposal, optical orthogonal frequency division multiplexing (O-OFDM) symbols are decomposed into multiple O-OFDM symbols that are in the linear range of the LEDs after adaptive iterative clipping according to the signal amplitude and clipping threshold, thereafter simultaneously transmitted through multiple LEDs. When the number of LEDs is large enough, clipping distortion can be significantly reduced or even completely eliminated. In addition, the all-zero symbols existing in the ISC scheme can be completely eliminated. Experimental results show that the proposed scheme can not only improve the BER performance and the optical power efficiency, but also reduce the clipping times compared with the traditional ISC scheme. Besides,our proposed scheme could achieve higher information transmission rate compared with the ASDST scheme.
Knowledge on and preventive practices of cholera in Al-Mahweet – Yemen, 2018: a cross-sectional study
Authors: Dureab Fekri , Al-Qadasi Yasameen , Nasr Hani , Al-Zumair Marwah , Al-Mahbashi Taha 
Published: October 2021

Abstract
Yemen has experienced one of the world's worst cholera outbreaks in the recent history of cholera records. This study aims to identify knowledge and practices among people of Al-Mahweet governorate toward cholera infection, which can play a critical role in reducing cholera morbidity and shaping the public health response. A cross-sectional study was conducted in an area of high cholera prevalence in 2018 using structured questionnaires. Most community respondents were able to correctly identify the symptoms and risk factors of cholera. While 65% of the respondents in this study knew that proper disposal of human waste is an essential measure of cholera prevention, only 11% of the respondents knew that proper washing of fruits and vegetables lowers the risk of cholera infection. About 62.5% of households did not treat water for safe drinking. Water was scarce in about 30% of households and near-home defecation was observed in about 23%. In conclusion, this study reveals several gaps in different aspects of hygienic and preventive practices including water treatment, waste disposal, and defecation practices. Cholera response should contain comprehensive health promotion interventions to improve the public's knowledge and enhance healthy practices. Stakeholders should support communities with sustainable water and sanitation systems.