bluuebunny/crossref_metadata_2025_split huggingface dataset:
Online Food Delivery Services Among Young Adults in Depok: Factors Affecting the Frequency of Online Food Ordering and Consumption of High-risk Food
Authors: Martha Evi , Ayubi Dian , Besral Besral , Rahmawati Nurul Dina , Mayangsari Aisyah Putri , Sopamena Yoslien , Astari Miranda , Zulfa Resvi Siti 
Published: December 2021

Abstract
BackgroundThe increasing desire of the community towards fulfilling the practical needs of life is currently accommodated by the rapid development of communication technology in the last five years in Indonesia. One example is the emergence of various food delivery applications. This will affect the pattern of daily food consumption in the community. Therefore, this study determined the personal and socio-environmental factors that influence the frequency of online food ordering (OFO) behavior and also high-risk food consumption through online food delivery services (OFDS) among young adults in Depok City. MethodsThis research was a cross-sectional study conducted with 686 participants of young adults aged 20–39 years that lived in Depok City, West Java Province, Indonesia, for minimal the last six months. They were administered a questionnaire through Alchemer, formerly SurveyGizmo. Descriptive statistics were employed for all variables measured. A T-test and a Chi-square test were conducted to determine the factors influencing the OFO frequency and high-risk food consumption through OFDS. Predictor variables were generated using multi-logistic regression models. The predictor for the frequency of OFO behavior was satisfaction toward OFDS (ß = 3.3; CI: 2.4–4.7; p-value = 0.000), while those for high-risk food consumption were personal and socio-environmental factors (ß = 1.8; CI: 1.3–2.5; p-value = 0.001 and ß = 1.7; CI: 1.2–2.4; p-value = 0.001, respectively).ResultsThe personal and socio-environmental factors related to the frequency of OFO behavior were attitudes toward risk, benefit, barriers, features of OFDS, satisfaction with OFDS, socio-environmental influence, perception about OFDS, and online food preference. Furthermore, the factors associated with high-risk food consumption through OFDS were attitudes toward benefit, features, satisfaction with OFDS, socio-environmental influence, perception about OFDS, online food preference, and knowledge of OFDS. ConclusionsSatisfaction, social environment, and food preference play essential roles in OFDS. The findings demonstrated the urgency of conducting further studies to elaborate on the association of OFDS with non-communicable diseases.
Prevalence and Associated Factors of Depression in Outpatients of Internal Medicine Department of Kigali University Teaching Hospital, Rwanda
Authors: Gafaranga Jean Pierre , Bitunguhari Leopold , Mudenge Charles , Kelly Brian 
Published: December 2021

Abstract
Background
Depression is more prevalent in medically ill patients than in the general population. More is not known about depression rates in internal medicine outpatients consulting the teaching hospitals in Rwanda. Adults with medical diseases are more likely to develop depression, it is an often-unrecognized co-morbidity in this group, and it has a major impact on their function and disability. In the absence of studies in our region, we did this research in our country with interest in what factors might be associated with depressive symptoms.
Methods
The descriptive cross-sectional study was used to determine the prevalence of depression and associated factors among internal medicine outpatients of Kigali university teaching hospital (CHUK). The 9 items Patient Health Questionnaire (PHQ-9) instrument was used to screen depression symptoms among outpatients. The validated cut-off score of 10 for diagnosing depression was applied in this study. Patients’ clinical and socio-demographic characteristics were collected and analyzed for their relationship with depression. All patients who scored PHQ-9 above 10 were referred to CHUK mental health department for appropriate management.
Results
Three hundred patients were recruited, of whom 65.3%) were females and 51% had an age between 45 and 96 years. The overall prevalence of depression among outpatients in the internal medicine department of CHUK was 45.7%. Outpatients had 20.7%, 17%, and 10% for moderate, moderately severe, and severe depression, respectively. Age, educational status, and follow-up visits as current physical complaints were independently associated with depression (P < 0.05). As much as 22.7% of patients were presenting with Suicidal ideas. The study showed that low income was strongly associated with suicide ideation (P < 0.001). Regardless of age and gender, the prevalence of depression was higher among outpatients of internal medicine than the general population.
Conclusions
A holistic approach in the management of internal medicine outpatients should be implemented to facilitate the early detection and treatment of depression in general tertiary hospitals. Furthermore, intervention programs that address depression and suicide in adults are needed.
Myomaker and Myomixer Characterization in Gilthead Sea Bream Under Different Myogenesis Conditions.
Authors: Perelló-Amorós Miquel , Otero-Tarrazón Aitor , Jorge-Pedraza Violeta , García-Pérez Isabel , Sánchez-Moya Albert , Gabillard Jean-Charles , Moshayedi Fatemeh , Navarro Isabel , Capilla Encarnación , Fernández-Borràs Jaume , Blasco Josefina , Josep Chillarón , serrana Daniel García de la , Gutiérrez Joaquim 
Published: December 2021

Abstract
The skeletal muscle is formed by multinucleated myofibers originated by waves of hyperplasia and hypertrophy during myogenesis. Tissue damage triggers a regeneration process including new myogenesis and muscular remodeling. During myogenesis, the fusion of myoblasts is a key step that requires different genes’ expression, including the fusogens Myomaker and Myomixer. The present work aimed to characterize these proteins in gilthead sea bream and their possible role in in vitro myogenesis, at different fish ages and during muscle regeneration after induced tissue injury. Myomaker is a transmembrane protein highly conserved among vertebrates; whereas Myomixer is a micropeptide that is moderately conserved but maintains its crucial AxLyCxL motif. myomaker expression is restricted to skeletal muscle, while the expression of myomixer is more ubiquitous. In primary myocytes culture, myomaker and myomixer expression peaked at day 6 and day 8, respectively. During regeneration, the expression of both fusogens and all the myogenic regulatory factors showed a peak after 16 days post-injury. Moreover, myomaker and myomixer were present at different ages, but in fingerlings there were significantly higher transcript levels than in juveniles or adult fish. Overall, Myomaker and Myomixer are valuable markers of muscle growth that together with other regulatory molecules, can provide a deeper understanding of myogenesis regulation in fish.
Polymerized Porin as a Novel Delivery Platform for Coronavirus Vaccine
Authors: Yang Zhongqian , Hua Liangqun , Yang Mengli , Li Weiran , Ren Zhaoling , Zheng Xiao , Chen Haoqian , Long Qiong , Bai Hongmei , Hang Weiwei , Ma Yanbing 
Published: December 2021

Abstract
Coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), seriously threatens human life and health. The correct folding and polymerization of the receptor-binding domain (RBD) protein of coronavirus in Escherichia coli may reduce the cost of SARS-CoV-2 vaccines. Here, we designed this nanopore by using the principle of ClyA porin polymerization triggered by the cell membrane. We use surfactants to "pick" the ClyA-RBD nanopore from the bacterial outer membrane in this study. More importantly, the polymerized RBD displayed on ClyA-RBD polymerized porin (RBD-PP) already has some correct spatial structures of virus spikes. The nanostructures of RBD-PP can target lymph nodes and promote antigen uptake and processing by dendritic cells, thereby effectively eliciting the production of anti-SARS-CoV-2 neutralizing antibodies and systemic cellular immune responses and immune memory. We applied ofthis PP-based vaccine platform to make an RBD-based subunit vaccine against SARS-CoV-2, which will provide a foundation for the development of inexpensive coronavirus vaccines. The development of novel vaccine delivery system is an important part of innovative drug research. This novel PP-based vaccine platform is likely to be applied to more fields, including other viral vaccines, bacterial vaccines, tumor vaccines, drug delivery, and disease diagnosis.
Inhibition of Salmonella enterica serovar Typhimurium Type Ⅲ Secretion System and Infection Using Small Molecule Quercitrin
Authors: Li Qingjie , Wang Lianping , Liu Shuang , Xu Jingwen , Song Zeyu , Chen Tingting , Deng Xuming , Lv Qianghua 
Published: December 2021

Abstract
AimsThis study was conducted to screen the type Ⅲ secretion system (T3SS) inhibitors of Salmonella enterica serovar Typhimurium (S. Typhimurium) from natural compounds. Through systemic analysis the pharmacological activity and action mechanism of candidate compounds in vivo and in vitro. Methods and resultsUsing an effector-β-lactamase fusion reporter system in S. Typhimurium, we discovered that quercitrin could block effector SipA translocation into eukaryotic host cell without affecting bacterial growth, and inhibit invasion or epithelial cells damage. Using β-galactosidase activity and Western blot assay, it was found that quercitrin significantly inhibits the expression of SPI-1 genes (hilA and sopA) and effectors (SipA and SipC). The animal experiment results indicated that quercitrin reduces mortality, pathological damages and colony colonization of infected mice. ConclusionsSmall-molecule inhibitor quercitrin directly inhibits the founction of T3SS in S. Typhimurium, and provids a potential alternative antimicrobial against Salmonella infection.Significance and impact of the studyNatural compounds have become valuable resources for antibacterials discovery due to their widely structures and biological activities. However, the potential targets and molecular action mechanisms of candidate compounds responsible for anti-infections remain elusive. The T3SS plays a crucial role in bacterial invasion and pathogenesis process in S. Typhimurium. Compared with traditional antibiotics, small molecular compounds can inhibit the T3SS of Salmonella and achieve the effect of anti-infection. They have less pressure on bacterial survival and are not easy to produce drug resistance. This provides strong evidence for development novel anti-virulence drugs against Salmonella infection.
Quantitative Proteomic Analysis of Bi Zhong Xiao Decoction Against Collagen-induced Arthritis Rats in the Early and Late Stages
Authors: He Cailin , Wang Yang , Wen Yuqi , Li Teng , Hu En , Zeng Siqing , Xiong Xingui 
Published: December 2021

Abstract
Background: Rheumatoid arthritis (RA) is a chronic, progressive, systemic autoimmune inflammatory disease. Bi Zhong Xiao decoction (BZXD) performs multiple functions for rheumatoid arthritis (RA) treatment for decades. In this study, we aimed to study the protein alterations of BZXD in the early and late stages of RA.Methods: Sprague-Dawley rats were randomly divided into the Control, collagen-induced arthritis (CIA) and BZXD groups. Clinical assessment, paw thickness, weight changes and serum inflammatory cytokine levels were used to evaluate anti-inflammatory effects. Histopathological tests were performed to assess the improvement of inflammation and synovial hyperplasia. Moreover, we analyzed the proteins profiling of synovial tissue samples with different time intervals after BZXD treatment by Isobaric Tag for Relative Absolute (ITRAQ) quantitative proteomics technology. To further explore the interrelationships among differentially expressed proteins (DEPs), we used DAVID Bioinformatics Resources v6.8 and STRING 11.0 for bioinformatics analysis. Besides, western blot was exerted to verify related proteins.Results: In our study, BZXD ameliorated joint inflammation, suppressed the pathological changes in arthrosis of CIA rats. The proteomic analysis demonstrated that CIA rats were mainly involved in two significant pathways (the focal adhesion and the ECM-receptor interaction) in the early stage. BZXD down-regulated the expression of proteins involved in these pathways, such as CAV1, CHAD, COL3A1, COL5A2, COL6A1 and COL6A5. Additionally, BZXD exerts anti-inflammatory effects in the late stage mainly by increasing the expression of FASN, and affecting fatty acid metabolism.Conclusion: BZXD exerts therapeutic effects on RA through multi-pathways in the early and late stages. This work may provide proteomic clues for treating RA by BZXD.
Dry EEG Measurement of P3 to Evaluate Cognitive Load During Sitting, Standing, and Walking
Authors: Swerdloff Margaret , Hargrove Levi 
Published: December 2021

Abstract
The impact of cognitive load on individuals with motor impairments is poorly understood. Cognitive load has been studied using subjective assessments, dual-task studies, physiological measures, and clinical metrics, which are specific to the motor task being performed and do not measure brain signals directly. Combining brain imaging with dual-task paradigms provides a quantitative, direct metric of cognitive load that is agnostic to the motor task. To better understand the impact of cognitive load during activities of daily living, we measured brain activity from a dry EEG headset as participants attended to an auditory stimulus paradigm during sitting, standing, and walking. The stimulus paradigm consisted of an auditory oddball task in which they had to report the number of oddball tones that were heard during each task. The P3 event-related potential, which is sensitive to cognitive load, was extracted from EEG signals in each condition. Results showed that P3 was significantly lower during walking compared to sitting (p = .039), indicating that cognitive load was higher during walking compared to the other activities. No significant differences in P3 were found between sitting and standing. Head motion did not have a significant impact on the measurement of cognitive load. These results encourage the use of a dry EEG system to further investigate cognitive load during dynamic activities in individuals with and without motor impairments.
Developing Landslide Hazard Scenario of Kashmir Himalaya from the Historical Events
Authors: shah Bilquis , Bhat M. Sultan , Alam Akhtar , Sheikh Hilal , Ali Noureen 
Published: December 2021

Abstract
Kashmir Himalaya being a rugged and tectonically active zone has complex, unstable geology along with steep slopes, creating a favorable environment for landslide hazards, especially along the National Highway (NH-44) that connects the Vale of Kashmir with the rest of India. The historical landslide database for the whole country has not yet been developed and the data provided by various government organizations are often very limited because most of the time local and small-scale landslide events do not get recorded, thus, leading to misinterpretations. The present study focuses on retrieving the information on landslide events and their impacts to develop a comprehensive database for the period from 1990 to 2020 in Jammu and Kashmir, emphasizing Jammu-Srinagar National Highway (NH-44). A hotspot analysis tool (Getis-ord-Gi* algorithm) was used to understand the spatial distribution and concentration of the events throughout the region. The annual and seasonal analysis of the 739 landslide events reported in the valley for the selected period suggests an increasing trend causing 1000 fatalities and 267 injuries. The findings show that out of 20 districts, 16 are relatively more exposed to landslides and the socio-impact induced by landslides was found more along the NH-44 with 303 landslide occurrences reported in 260 days in the past three decades having a high intensity of damage and loss. The results of this study are expected to be of potential use for developing a Landslide Early Warning System (LEWS) and for mitigating the impacts of landslides in the Kashmir Himalaya.
A Novel Decentralized Fixed-Time Tracking Control for Modular Robot Manipulators: Theoretical and Experimental Verification
Authors: Lu Zengpeng , Li Yuanchun , Li Yan 
Published: December 2021

Abstract
This paper presents a novel decentralized fixed-time tracking control approach, which realizes the advantages of modular robot manipulators (MRMs) with fixed-time convergence, strong robustness, and high tracking performance. First, to estimate the total uncertainty of MRMs, the fixed-time observer based on the extended state is developed. Then, combined with the disturbance observer, a novel decentralized control method based on a fixed-time control strategy was devised to accomplish global fixed-time convergence of MRMs. And, stability analysis based on Lyapunov is utilized to obtain the fixed-time stability as well as convergence time of MRMs. Finally, numerical analysis and experiment respectively verify the excellent tracking ability of the presented decentralized fixed-time tracking control.
Parental Segregation Study Reveals Rare Benign and Likely Benign Variants in a Brazilian Cohort of Rare Diseases
Authors: Quaio Caio Robledo D' Angioli Costa , Ceroni Jose Ricardo Magliocco , Cervato Murilo Castro , Thurow Helena Strelow , Moreira Caroline Monaco , Trindade Ana Carolina Gomes , Furuzawa Cintia Reys , Souza Rafaela Rogerio Floriano , Perazzio Sandro Felix , Dutra Aurelio Pimenta , Chung Christine Hsiaoyun , Kim Chong Ae 
Published: December 2021

Abstract
Genomic studies may generate massive amounts of data, bringing interpretation challenges. Efforts for the differentiation of benign and pathogenic variants gain importance.In this article, we used segregation analysis and other molecular data to reclassify to benign or likely benign several rare clinically curated variants of autosomal dominant inheritance from a cohort of 500 Brazilian patients with rare diseases.This study included only symptomatic patients who had undergone molecular investigation with exome sequencing for suspected diseases of genetic etiology. Variants clinically suspected as the causative etiology and harbored by genes associated with highly-penetrant conditions of autosomal dominant inheritance underwent Sanger confirmation in the proband and inheritance pattern determination because a “de novo” event was expected.Among all 327 variants studied, 321 variants were inherited from asymptomatic parents. Considering segregation analysis, we have reclassified 51 rare variants as benign (n=51) and 211 as likely benign (n=211).In our study, the inheritance of a highly penetrant variant expected to be de novo for pathogenicity assumption was considered as a non-segregation and, therefore, a key step for benign or likely benign classification. Studies like ours may help to identify rare benign variants and improve the correct interpretation of genetic findings.
Bayesian Parametric Modeling of Time to Tuberculosis Co-Infection of HIV/AIDS Patients Under Antiretroviral Therapy Treatment at Jimma University Medical Center, Ethiopia
Authors: Umeta Abdi Kenesa , Yermosa Samuel Fikadu , Dufera Abdisa G. 
Published: December 2021

Abstract
Background: Tuberculosis is the most common opportunistic infection among HIV/AIDS patients, including those following Antiretroviral Therapy treatment. The risk of Tuberculosis infection is higher in people living with HIV/AIDS than in people who are free from HIV/AIDS. Many studies focused on prevalence and determinants of Tuberculosis in HIV/AIDS patients without taking into account the censoring aspects of the time to event data. Therefore, this study was undertaken with aim to model time to Tuberculosis co-infection of HIV/AIDS patients following Antiretroviral Therapy treatment using Bayesian parametric survival models.Methods: A data of a retrospective cohort of HIV/AIDS patients under Antiretroviral Therapy treatment follow-up from January 2016 to December 2020 until Tuberculosis was clinically diagnosed or until the end of the study was collected from Antiretroviral Therapy treatment center of Jimma University Medical Center, Ethiopia. In order to identify the risk factors which have association with Tuberculosis co-infection survival time, Bayesian parametric Accelerated failure time survival models were fitted to the data using Integrated Nested Laplace Approximation methodology.Results: About 26.37% of the study subjects had been co-infected with tuberculosis during the study period. Among the parametric Accelerated failure time models, the Bayesian log-logistic Accelerated failure time model was found to be the best fitting model for the data.Conclusions: Tuberculosis co-infection survival time was significantly associated with place of residence, smoking, drinking alcohol, family size, WHO clinical stages, functional status, CD4 count, BMI and hemoglobin level. The finding of this study provide timely information on the risk factors associated with TB co-infection survival time for healthy policy makers and planners.
Ragweed Pollen Concentration Predict Seasonal Oculo-rhinitis and Asthma Severity in Subjects Allergic to Ragweed.
Authors: Bonini Maira , MONTI GIANNA , PELAGATTI MATTEO , CERIOTTI VALENTINA , RE ELISABETTA , BRAME' BARBARA , BOTTERO PAOLO , TOSI ANNA , VAGHI ADRIANO , MARTELLI ALBERTO , TRAINA GIOVANNI , RIVOLTA LOREDANA , RIVOLTA FEDERICA , ORTOLANI CLAUDIO MARIA 
Published: December 2021

Abstract
Objectives: 1. To investigate the correlation between ragweed pollen concentration and conjunctival, nasal and asthma symptoms severity in patients allergic to ragweed using ambient pollen exposure in the Milan area during the 2014 ragweed season; 2. to calculate the pollen / symptom thresholds and 3. to assess the effectiveness of ragweed Allergen Immuno Therapy (AIT).Patients: 66 subjects allergic to Amb a 1 enrolled in the study and were divided into two cohorts: AIT treated (24) and non-AIT treated (42).Measurements: Pollen counts and daily symptom/medication patient diaries. Autoregressive Distributed Lag Models were used to develop predictive models of daily symptoms and to evaluate the short-term effects of temporal variations in pollen concentration on the onset of symptoms. Results: We found significant correlations between ragweed pollen load and the intensity of symptoms, for all three symptom categories respectively, both in non-AIT treated (𝛕= 0.341, 0.352, 0.721 and ρ = 0.48, 0.432, 0.881, p-value < 0.001) and in AIT treated patients (O= 0.46, 0.610, 0.66 and ρ = 0.692, 0.805, 0.824; p-value < 0.001). In both cohorts, we observed a positive correlation between the number of symptoms reported and drug use. Mean symptom levels were significantly greater in non-AIT treated than in AIT treated patients (p < 0.001) for all symptom categories. Pollen concentration thresholds for three symptom severity levels were calculated.Conclusions: Ragweed pollen concentration is predictive of symptom severity in ragweed (Amb a 1) allergy patients. AIT treated patients had significantly reduced mean symptom levels compared to non-AIT patients.
The Integrated Optokinetic Nystagmus Inter-saccadic Interval Scales Statistical Different In a Group of Vertigo Patients Depended On The Stimulation Velocity, But Not For Healthy Subjects
Authors: Aasen Torbjørn 
Published: December 2021

Abstract
Optokinetic nystagmus is rhythmic eye movements, back and forth, with a slow and fast phase, when the eyes are presented for full-field visual stimulus. OKN was recorded in 20 healthy subjects and 20 patients suffering from vertigo, for four conditions: stripes moving 30 o/s left and right and 60 o/s left and right. Calculating the scaling, the spread over time, for the integrated optokinetic nystagmus inter-saccadic interval, the time intervals between the onsets of consecutive fast components, shows lower Hurst exponent for velocity stimulation of 30o/s compared to 60o/s for both patients and health subjects, but only reach statistical differences for the group of patients.