bluuebunny/crossref_metadata_2025_split huggingface dataset:
Plasma-Treated Water Affects Listeria monocytogenes Vitality and Biofilm Structure
Authors: Handorf Oliver , Pauker Viktoria Isabella , Weihe Thomas , Schäfer Jan , Freund Eric , Schnabel Uta , Bekeschus Sander , Riedel Katharina , Ehlbeck Jörg 
Published: April 2021

Background: Plasma-generated compounds (PGCs) such as plasma-processed air (PPA) or plasma-treated water (PTW) offer an increasingly important alternative for the control of microorganisms in hard-to-reach areas found in several industrial applications including the food industry. To this end, we studied the antimicrobial capacity of PTW on the vitality and biofilm formation of Listeria monocytogenes, a common foodborne pathogen.Results: Using a microwave plasma (MidiPLexc), 10 ml of deionized water was treated for 100, 300, and 900 s (pre-treatment time), after which the bacterial biofilm was exposed to the PTW for 1, 3, and 5 min (post-treatment time) for each pre-treatment time, separately. Colony-forming units (CFU) were significantly reduced by 4.7 log10 ± 0.29 log10, as well as the metabolic activity decreased by 47.9 ± 9.47% and the cell vitality by 69.5 ± 2.1%, compared to the control biofilms. LIVE/DEAD staining and fluorescence microscopy showed a positive correlation between treatment and incubation times, as well as reduction in vitality. Atomic force microscopy (AFM) indicated changes in the structure quality of the bacterial biofilm.Conclusion: These results indicate a promising antimicrobial impact of plasma-treated water on Listeria monocytogenes, which may lead to more targeted applications of plasma decontamination in the food industry in the future.
Pleural Fluid Has Pro-Growth Biological Properties Which Enable Cancer Cell Proliferation
Authors: Asciak Rachelle , Kanellakis Nikolaos I. , Yao Xuan , Abd Hamid Megat , Mercer Rachel M. , Hassan Maged , Bedawi Eihab O. , Dobson Melissa , Fsadni Peter , Montefort Stephen , Dong Tao , Rahman Najib M. , Psallidas Ioannis 
Published: April 2021

ObjectivesPatients with malignant pleural mesothelioma (MPM) or pleural metastases often present with malignant pleural effusion (MPE). This study aimed to analyze the effect of pleural fluid on cancer cells.Materials and MethodsEstablished patient-derived cancer cell cultures derived from MPE (MPM, breast carcinoma, lung adenocarcinoma) were seeded in 100% pleural fluid (exudate MPM MPE, transudate MPE, non-MPE transudate fluid) and proliferation was monitored. In addition, the establishment of new MPM cell cultures, derived from MPE specimens, was attempted by seeding the cells in 100% MPE fluid.ResultsAll established cancer cell cultures proliferated with similar growth rates in the different types of pleural fluid. Primary MPM cell culture success was similar with MPE fluid as with full culture medium.ConclusionsPleural fluid alone is adequate for cancer cell proliferation in vitro, regardless of the source of pleural fluid. These results support the hypothesis that pleural fluid has important pro-growth biological properties, but the mechanisms for this effect are unclear and likely not malignant effusion specific.
Laparoscopic vs. Transabdominal Treatment for Overflow Fecal Incontinence Due to Residual Aganglionosis or Transition Zone Pathology in Hirschsprung's Disease Reoperation
Authors: Chen Feng , Wei Xiaoyu , Chen Xiaohua , Xiang Lei , Feng Jiexiong 
Published: April 2021

Objective: The aim of this study was to describe the details of laparoscopic-assisted reoperative surgery for Hirschsprung's disease (HSCR) with overflow fecal incontinence, and to retrospectively compare laparoscopic-assisted surgery with transabdominal pull-through surgery.Methods: We retrospectively analyzed patients with HSCR with overflow fecal incontinence after the initial surgery in our center between January 2002 and December 2018. Pre-operative, peri-operative, and post-operative data were recorded for statistical analysis.Results: Thirty patients with overflow fecal incontinence after initial megacolon surgery [17 who underwent transanal pull-through (TA-PT) and 13 who underwent laparoscopic-assisted pull-through (LA-PT)] required a secondary surgery [reoperation with LA-PT (LAR-PT) (n = 16) or reoperation with transabdominal pull-through (TR-PT) (n = 14)]. Indications for reoperation were residual aganglionosis (RA) (7/30, 23.3%) or transition zone pathology (TZP) (23/17, 76.7%). Blood loss was significantly decreased in the LAR-PT group (75 ± 29.2 ml) compared to the TR-PT group (190 ± 51.4 ml) (P = 0.001). The length of hospital stay was significantly shorter in the LAR-PT group (10 ± 1.5 days) than that in the TR-PT group (13 ± 2.4 days). No significant differences were found between two groups in surgical methods, defecation function score, or post-operative complications except for wound infection (LAR-PT vs. TR-PT 0 vs. 28.6%, P &lt; 0.05).Conclusions: It is necessary to make a comprehensive analysis of the causes of fecal incontinence after HSCR surgery and make an accurate judgment using appropriate methods. If a reoperation was inevitable for patients with overflow fecal incontinence due to RA or TZP, a comprehensive evaluation prior to the operation is required to maximize the benefit from reoperation. Although laparoscopic reoperation with heart-shaped anastomosis was safe and feasible for patients with failed initial Soave technique, unnecessary reoperation should be avoided as much as possible.
Cannabidiol Isolated From Cannabis sativa L. Protects Intestinal Barrier From In Vitro Inflammation and Oxidative Stress
Authors: Cocetta Veronica , Governa Paolo , Borgonetti Vittoria , Tinazzi Mattia , Peron Gregorio , Catanzaro Daniela , Berretta Massimiliano , Biagi Marco , Manetti Fabrizio , Dall’Acqua Stefano , Montopoli Monica 
Published: April 2021

The relevance and incidence of intestinal bowel diseases (IBD) have been increasing over the last 50 years and the current therapies are characterized by severe side effects, making essential the development of new strategies that combine efficacy and safety in the management of human IBD. Herbal products are highly considered in research aimed at discovering new approaches for IBD therapy and, among others, Cannabis sativa L. has been traditionally used for centuries as an analgesic and anti-inflammatory remedy also in different gastrointestinal disorders. This study aims to investigate the effects of different C. sativa isolated compounds in an in vitro model of intestinal epithelium. The ability of treatments to modulate markers of intestinal dysfunctions was tested on Caco-2 intestinal cell monolayers. Our results, obtained by evaluation of ROS production, TEER and paracellular permeability measurements and tight junctions evaluation show Cannabidiol as the most promising compound against intestinal inflammatory condition. Cannabidiol is able to inhibit ROS production and restore epithelial permeability during inflammatory and oxidative stress conditions, suggesting its possible application as adjuvant in IBD management.
The Endocannabinoid Anandamide Attenuates Acute Respiratory Distress Syndrome by Downregulating miRNA that Target Inflammatory Pathways
Authors: Sultan Muthanna , Alghetaa Hasan , Mohammed Amirah , Abdulla Osama A. , Wisniewski Paul J. , Singh Narendra , Nagarkatti Prakash , Nagarkatti Mitzi 
Published: April 2021

Acute respiratory distress syndrome (ARDS) is defined as a type of respiratory failure that is caused by a variety of insults such as pneumonia, sepsis, trauma and certain viral infections. In this study, we investigated the effect of an endocannabinoid, anandamide (AEA), on ARDS induced in the mouse byStaphylococcusEnterotoxin B (SEB). Administration of a single intranasal dose of SEB in mice and treated with exogenous AEA at a dose of 40 mg/kg body weight led to the amelioration of ARDS in mice. Clinically, plethysmography results indicated that there was an improvement in lung function after AEA treatment accompanied by a decrease of inflammatory cell infiltrate. There was also a significant decrease in pro-inflammatory cytokines IL-2, TNF-α, and IFN-γ, and immune cells including CD4+T cells, CD8+T cells, Vβ8+T cells, and NK+T cells in the lungs. Concurrently, an increase in anti-inflammatory phenotypes such as CD11b + Gr1+ Myeloid-derived Suppressor Cells (MDSCs), CD4 + FOXP3 + Tregs, and CD4+IL10 + cells was observed in the lungs. Microarray data showed that AEA treatment in ARDS mice significantly altered numerous miRNA including downregulation of miRNA-23a-3p, which caused an upregulation of arginase (ARG1), which encodes for arginase, a marker for MDSCs, as well as TGF-β2, which induces Tregs. AEA also caused down-regulation of miRNA-34a-5p which led to induction of FoxP3, a master regulator of Tregs. Transfection of T cells using miRNA-23a-3p or miRNA-34a-5p mimics and inhibitors confirmed that these miRNAs targeted ARG1, TGFβ2 and FoxP3. In conclusion, the data obtained from this study suggests that endocannabinoids such as AEA can attenuate ARDS induced by SEB by suppressing inflammation through down-regulation of key miRNA that regulate immunosuppressive pathways involving the induction of MDSCs and Tregs.
REL-1017 (Esmethadone) Increases Circulating BDNF Levels in Healthy Subjects of a Phase 1 Clinical Study
Authors: De Martin Sara , Gabbia Daniela , Folli Franco , Bifari Francesco , Fiorina Paolo , Ferri Nicola , Stahl Stephen , Inturrisi Charles E. , Pappagallo Marco , Traversa Sergio , Manfredi Paolo L. 
Published: April 2021

Brain-derived neurotrophic factor (BDNF), a neurotrophin widely expressed in the central nervous system, exhibits important effects on neural plasticity. BDNF has been implicated in the mechanism of action of ketamine, a N-methyl-d-aspartic acid receptor (NMDAR) antagonist with rapid anti-depressant effects in humans. REL-1017 (esmethadone), the d-optical isomer of the racemic mixture d-l-methadone, is devoid of clinically relevant opioid activity at doses expected to exert therapeutic NMDAR antagonistic activity in humans. The present study was conducted to ascertain the effects of oral administration of 25 mg of REL-1017 for 10 days on plasma BDNF in healthy subjects confined to an inpatient unit for a phase 1 clinical trial. We observed an increase in post-treatment BDNF plasma levels compared to pre-treatment levels. Post-treatment, Day 10 BDNF plasma levels ranged from 2 to 17 times pre-treatment levels in the 25 mg REL-1017 treatment group, whereas in the placebo group, BDNF plasma levels remained unchanged (p = 0.028). Diastolic blood pressure decreased significantly in subjects treated with REL-1017, while no effect could be observed in the placebo group. In conclusion, the administration of 25 mg REL-1017 significantly increased BDNF plasma levels and significantly decreased diastolic blood pressure in healthy subjects confined to an inpatient unit for a phase 1 clinical trial.
Non-medical Use of Prescription Gabapentinoids (Gabapentin and Pregabalin) in Five European Countries
Authors: Fonseca Francina , Lenahan William , Dart Richard C. , Papaseit Esther , Dargan Paul I. , Wood David M. , Guareschi Marilena , Maremmani Icro , Auriacombe Marc , Farré Magí , Scherbaum Norbert , Torrens Marta 
Published: April 2021

Background: Non-medical use (NMU) of prescription GABA analogs (pregabalin and gabapentin) has been reported especially in opiate dependent persons. However, by now the prevalence of NMU of gabapentinoids in the general population has not been sufficiently evaluated. The aim of this research paper is to determine the prevalence of prescription GABA analog NMU and associated demographics in five European countries with special detail of Spain.Methods: The RADARS Survey of Non-Medical Use of Prescription Drugs Program (NMURx) is a harmonized series of contemporaneous cross-sectional surveys of adults conducted in multiple countries. NMURx collects data from the general population in each participating country about NMU of prescription drugs, illicit drugs, and associated demographics. NMU was defined as “using a medication without a doctor's prescription or for any reason other than what was recommended by their doctor.” Responses from Spain (4Q2017, n=10,062) were analyzed in detail. Comparative data were available from France, Germany, Italy, and UK. Responses were collected using non-probability quota sampling and post-stratification population weighting was applied to reflect the national distributions of adults, based on age, gender, and census region. Rates of NMU and associated demographics were reported as rate of past 90-day NMU per 100,000 adult population with 95% confidence intervals.Results: Germany (1,197 per 100,000 adult population [95% CI: 1,004.3–1,379.1]) and United Kingdom (1,067 per 100,000 adult population [95% CI: 851.3–1,283.2]) presented the highest prevalence of gabapentinoids NMU. In Spain the prevalence of past 90 days GABA analog NMU was: 344.4, 95% (CI 204.8–484.0), with male predominance. Those who non-medically use GABA analogs had a higher prevalence of lifetime chronic pain, lifetime illicit drug use, and previous substance abuse treatment. In Spain, 20% of respondents who ever have used gabapentinoids, reported a lifetime NMU; the prevalence was higher for pregabalin 624 (6.2%) than for gabapentin 444 (4.4%). The main reasons for use were to self-treat pain and other medical conditions.Conclusions: The risk of NMU of gabapentinoids should not be neglected. Subjects with a history of chronic pain and lifetime substance use disorders had an increased risk of NMU of gabapentinoids.
Ly6chi Infiltrating Macrophages Promote Cyst Progression in Injured Conditional Ift88 Mice
Authors: Aloria Ernald Jules G. , Song Cheng J. , Li Zhang , Croyle Mandy J. , Mrug Michal , Zimmerman Kurt A. , Yoder Bradley K. 
Published: April 2021

Key Points

Ly6chi infiltrating macrophage numbers are increased in injured, conditional Ift88 mice, compared with controls.Loss of Ly6chi infiltrating macrophages slows injury-accelerated cystic disease.Ly6chi infiltrating macrophages drive cystic disease in non-Pkd1–deficient cystic models.
Autosomal Dominant Polycystic Kidney Disease Does Not Significantly Alter Major COVID-19 Outcomes among Veterans
Authors: Cui Xiangqin , Gallini Julia W. , Jasien Christine L. , Mrug Michal 
Published: April 2021

Key Points

Autosomal dominant polycystic kidney disease (ADPKD) was not a significant, independent risk factor for the four major outcomes studied among veterans with confirmed coronavirus disease 2019 (COVID-19).ADPKD did not significantly increase the risk for newly starting dialysis (after controlling for CKD) among veterans positive for COVID-19.The established risk factors for severe COVID-19 illness had significant effects in this cohort (e.g., type 2 diabetes and Black race).
اثر الظروف الاستثنائية على الحريات الشخصية
Authors: طالب مصدق عادل , جاسم الفارعة عبدالله 
Published: April 2021

قد تطرأ على الدولة ظروف استثنائية تهدد سلامتها، كالحروب والكوارث الطبيعية، وتجعلها عاجزة عن توفير حماية للنظام العام باستخدام القواعد والإجراءات العادية. وفي هذه الحالة لابد ان تتسع سلطات هيئات الضبط لمواجهة هذه الظروف، وذلك من خلال تمكينها من اتخاذ إجراءات سريعة وحازمة لمواجهتها.
ولما كانت الظروف الاستثنائية من شأنها المساس المباشر بحقوق وحريات الأفراد الشخصية التي يكفلها الدستور، فلابد أن يتدخل المشرع لتحديد القيود الواردة عليها. مع الاشارة على الضمانات لهذه الحريات من اجل أن لا تتعسف السلطة في اصدار قراراتها واوامرها بهذا الشأن.
السلطة التقديرية للقاضي في منح الأجل القضائي في دعوى التنفيذ
Authors: الخالدي حميد سلطان , حمود لؤي سطام 
Published: April 2021

تتمثل السلطة التقديرية للقاضي في منح الأجل القضائي في دعوى التنفيذ بامكانية تعديل أجل الالتزام العقدي، ذلك الأجل الذي اتفق الطرفان على تنفيذ الالتزام العقدي عند حلوله، إذ من المعلوم انهُ عند حلول أجل تنفيذ الالتزام يكون لزاماً على المدين ان يقوم بالوفاء الا ان هذا التنفيذ قد يكون مرهقاً لهُ، أو يسبب له ضرراً يجاوز ما يصيب الدائن من ضرر في ارجاء التنفيذ.
ويتمتع القاضي بسلطة تقديرية واسعة أثناء نظره في دعوى التنفيذ المعروضة امامه، في منح المدين المخل بالتزامه أجلاً قضائياً من أجل تنفيذ الالتزام، أو عدم منحه، حيث ان القاضي قد يرى ان عدم تنفيذ المدين لالتزامه، أو تأخره في ذلك يكون راجعاً لاسباب خارجة عن ارادته، وان هذه الاسباب قد تزول بعد وقت قصير، أو ان هذا التأخير كان لوقت قصير ولم يترتب عليه ضرر جسيم بالدائن وبالتالي فان القاضي يجد نفسه مدفوعاً لكي يعطي المدين أجلاً قضائياً ينفذ فيه التزامه، وذلك بباعث العدالة والرغبة في الحفاظ على الروابط التعاقدية قدر الامكان.
أصول الصياغة التشريعية للقاعدة الاجرائية الجزائية
Authors: حسين كاظم عبد الله , اسماعيل شاكر نوري 
Published: April 2021

يتكون البنيان القانوني للقواعد الاجرائية الجزائية من عنصرين الأول:هو المضمون أو الجوهر ويعد بمثابة المادة الأولية لتلك القواعد ويتمثل بالقيم التي يهدف المشرع حمايتها تحقيقا للمصلحة المرجوة منها ولا يمكن ذلك إلا من خلال وضع ذلك المضمون أو الجوهر بقوالب يمكن التعامل معها لتكون قابلة للتطبيق العملي وذلك بواسطة الصياغة التشريعية للقواعد القانونية وهذا العنصر الثاني الذي يكتمل به البناء القانوني للقواعد القانونية وتظهر به صورتها النهائية وتصبح القاعدة الاجرائية الجزائية قابلة للتطبيق العملي إذ أن الصياغة التشريعية تنقل جوهر القاعدة القانونية من حالتها النظرية الساكنة الى الحالة العملية ، ولغرض تحقيق الغاية التي وجدت القاعدة القانونية من أجلها لابد من إتباع أصول الصياغة التشريعية للقواعد القانونية وحسب السياسة الجنائية الاجرائية التي يتبعا المشرع ، إذ ان الصياغة التشريعية تختلف باختلاف السلطة أو الحق الذي يمنحه المشرع للمكلفين بالقاعدة الاجرائية الجزائية ، فتنقسم الصياغة التشريعية من حيث الأسلوب الى الصياغة الجامدة والصياغة المرنة ، وقد تكون الوسيلة المستخدمة في الصياغة مادية أو معنوية . لذا بينا في هذا البحث أصول الصياغة التشريعية في القواعد الاجرائية في قانون أصول المحاكمات الجزائية العراقي مع بيان بعض أوجة القصور في صياغة قواعده وتبين بأن المشرع العراقي قد أخذ بشتى اساليب الصياغة التشريعية فيها وبينا ما اتضح لنا من أوجه القصور فيها.
¿Construir o Adaptar Tests Psicológicos? Diferentes Respuestas a una Cuestión Controvertida.
Authors: Fernández Alberto , Pérez Edgardo , Alderete Ana María , Richaud María Cristina , Fernández Liporace Mercedes 
Published: April 2021

Durante el Congreso Argentino de Psicología del 2008, en la ciudad de Córdoba, se organizó un Simposio en el cual cuatro expertos (dos de Córdoba, dos de Buenos Aires) debatieron sobre la conveniencia de construir tests nuevos o adaptar los ya existentes. Nos pareció importante rescatar estas ponencias y ofrecérselas al lector de esta revista puesto que cada una de ellas expresa un posicionamiento diferente frente a esta problemática de notable vigencia en nuestro país. Podría decirse, de un modo muy simplificado, que el Profesor Pérez manifiesta su preferencia por la adaptación de tests, las Profesoras Richaud y Alderete expresan una postura más favorable a la construcción de nuevos instrumento y la Profesora Fernández Liporace opta por una posición intermedia. A continuación, se presentan las cuatro ponencias expuestas en el congreso y, finalmente, el Profesor Alberto Fernández suministra sus propias conclusiones respecto a la problemática en discusión.
DEFISIENSI COLLABORATIVE GOVERNANCE DALAM PENANGANAN PANDEMI COVID-19 DI INDONESIA
Authors: Purwaning Diah Martina , Arni Noventi Ike , Pratama M. Rizki 
Published: April 2021

AbstractThe Covid-19 pandemic is a shared problem so it must also be resolved collectively. In the context of crisis, the role of the government must remain dominant, but also by opening the door to collaborative governance. Therefore it is vital to understand the dynamics of the collaborative governance process in dealing with the Covid-19 pandemic which of course is full of challenges and obstacles in the midst of uncertainty. Researchers use the literature study method to track various conditions in an effort to resolve a pandemic that involves inter-sectoral efforts, but researchers find important points that must be corrected so that the handling of the pandemic does not experience deficiencies in collaborative governance. So far, real action is needed in improving the responsiveness of policies, quality of regulations, data management, monitoring systems and coordination among sectors. Keywords: collaborative governance; covid-19; pandemic AbstrakPandemi Covid-19 merupakan persoalan bersama sehingga juga harus diselesaikan secara bersama-sama pula. Dalam konteks krisis pun sebenarnya peran pemerintah harus tetap dominan akan tetapi juga dengan membuka pintu kolaborasi bersama dengan antar sektor (collaborative governance). Oleh karena itu penting untuk mengetahui dinamika proses collaborative governance dalam menangani pandemi Covid-19 yang tentu saja penuh dengan tantangan, kendala dan hambatan di tengah ketidakpastian. Peneliti menggunakan metode studi kepustakaan untuk melacak berbagai kondisi dalam usaha menyelesaikan pandemi yang melibatkan usaha antar sektor akan tetapi peneliti menemukan poin-poin penting yang harus diperbaiki agar penanganan pandemi tidak mengalami defisiensi dalam collaborative governance. Sejauh ini perlu tindakan nyata dalam memperbaiki responsivitas kebijakan, kualitas regulasi, manajemen data, sistem pengawasan dan koordinasi antar sektor. Kata Kunci: collaborative governance; covid-10; pandemi
horror se queda en casa
Authors: De León Francisco 
Published: April 2021

Este trabajo presenta una breve visión del cine de horror latinoamericano a partir del estudio de dos de las cintas más representativas de la región en las últimas décadas: Cronos (1992), del director mexicano Guillermo del Toro, y Juan de los muertos (2011), de Alejandro Brugués. A través de un análisis de las nociones de lo monstruoso y lo doméstico en ambas películas se trata de aproximar a las raíces ideológicas del horror en el cine latinoamericano. Y es que si bien este cine se nutre de las tradiciones fílmica y literaria europeas y norteamericanas, es indudable que ha generado todo un proceso que le otorga una identidad propia y original. Ambos casos, aunque separados por temas (el vampiro en el caso de Cronos y el zombi en Juan de los muertos), coinciden en sus visiones de lo familiar y lo doméstico alterados por la irrupción de lo monstruoso. Las cintas estudiadas brindan un crisol amplio de las estrategias más utilizadas en el cine del género.
monstruo en la obra de José María Merino
Authors: Noyaret Natalie 
Published: April 2021

“Cronista de lo inquietante, narrador de ciertas sombras invisibles”, Merino no podía dejar de recurrir al monstruo. Se dedica a ello no tanto en sus novelas como en sus cuentos y microrrelatos. En muchos textos, el monstruo está al servicio de una interrogación acerca de la identidad del sujeto contemporáneo. En otros, viene a alertarnos sobre ciertos aspectos deplorables de nuestra sociedad. En determinadas obras, Merino se encarga más particularmente de decirnos que el monstruo está en nosotros. El territorio de los sueños y del insomnio se revela también muy fértil para la aparición de monstruos, siendo estos la expresión en este caso de los temores profundos del escritor. En su último libro, El libro de las horas contadas, aparecen monstruos de los más pintorescos, y también de los más temibles, por eso mismo innombrables.
impostura del avatar: la belleza intelijente de Arturo Vallejo
Authors: Escadell Montiel Daniel 
Published: April 2021

El avatar como personificación del narrador en la blogoficción establece un modelo de comunicación con el conjunto de los lectores. El modelo impone, asimismo, un componente diegético vinculado a la máscara del personaje asumido por el autor. En este artículo analizamos el origen del avatar en la blogoficción a partir de los casos fundacionales del hoax digital en la sociedad-red y la posterior aplicación de los cánones de simulacro en la creación de Diario de una miss intelijente, blogonovela escrita por el autor español Arturo Vallejo.