bluuebunny/crossref_metadata_2025_split huggingface dataset:
Modeling microbial diversity across different soil ecosystems and environmental covariate selections in tropical SE Asian landscape
Authors: Wibowo Andri 
Published: April 2021

AbstractMicrobes play essential roles in the ecology of various environments and structure. Diversity of soil microbial communities is an important scientific interest in tropical landscape including in South East Asia. Soil environment in SE Asia is diverse and this influences the microbial diversity. Soil microbes in this study were collected using DNA isolation protocols and the whole microbial community structure of sampled soil was analyzed through next generation sequencing. Soil microbial identification was conducted by amplifying the 16S rRNA gene. Soils were sampled from 4 different environments in Sumatra and Kalimantan ecosystems representing plantation, swamp, peatland, and coastal ecosystems. Measured soil covariates including soil organic carbon C analyzed based on the Walkley-Black method and Kjeldahl method for N. Model of microbes in soil ecosystems were based on Akaike model selection (AIC) by testing the influence of soil covariates on soil microbial phylum. The microbial community was found to be comprising of a total number of 11 phyla. In soil of coastal ecosystem, microbial diversity at phylum level were dominated by Proteobacteria (82.3%), Actinobacteria (9.41%), Acidobacteria (4.7%), and the lowest was Bacteroidetes and Chloroflexi (0.58%). In plantation soil, the microbial abundance order was Firmicutes (41.6%) > Actinobacteria (29.7%) > Acidobacteria (20.0%) > Gemmatimonadetes (5.94%). The soil microbial abundance order in swamp was Proteobacteria (42.2%) > Acidobacteria (20.04%) > Bacteroidetes > (10.46%) > Actinobacteria (9.24%). In soil of peatland ecosystem, taxonomic assignments of microbial at the phylum level were dominated by Firmicutes (66.18%), Proteobacteria(16.94%), and Actinobacteria (16.87%). According to the values of AIC, Firmicutes was a microbial phylum that has high abundance in soil ecosystems influenced by pH covariates with AIC values of −6.54. Other soil covariates show less influence on Firmicutes with AIC values of −5.06. The combination model also show that pH cofactor was the important determinants with AIC values of −0.54 for Firmicutes (pH+C) and Firmicutes (pH+N) models. While AIC value for combination model of Firmicutes (C+N) only equals to 0.14.
Role of Ori inThermococcus barophilus
Authors: Moalic Yann , Catchpole Ryan , Leroy Elodie , Teer Logan Mc , Cueff-Gauchard Valérie , Aubé Johanne , Lu Yang , Roussel Erwan , Oberto Jacques , Flament Didier , Dulermo Rémi 
Published: April 2021

SummaryThe mechanisms underpinning replication of genomic DNA in Archaea have recently been challenged. Species belonging to two different taxonomic orders grow well in the absence of an origin of replication, challenging the role of the replication origin in these organisms. Here, we pursue the investigation of the particular way some archaea manage their DNA replication withThermococcus barophilusand the role of Ori in this Archaea. Surprisingly we discovered thatT. barophilususes its Ori all along the growth curve with marked increase at the end of exponential phase. Through gene deletion, we show that Ori utilization requires Cdc6, and that origin deletion results in increased time in lag phase and a moderate decrease of growth rate in mutants. The number of chromosomes are quite similar between both strains during exponential and early stationary phases but differs after 24h of growth where ΔTbOriC has only 6 chromosomes/cell compared to 10 for the reference strain (WT). Following 1hr of growth in fresh media, ΔTbOriC strains contains 3 chromosome copies/cell, whereas the WT contains only 1. We hypothesize that theT. barophilusmight degrade DNA to obtain energy to start replication and cell division, whereas the ΔTbOriC must maintain more chromosomal copies in order to initiate DNA replication in the absence of an origin or replication. Finally, we analyzed the role of Ori at temperatures above or below the optimal temperature, revealing that Ori is important to start growth at those temperatures, suggesting that replication origins may be involved in stress response.
ElectroEncephaloGraphy robust statistical linear modelling using a single weight per trial
Authors: Pernet Cyril , Rousselet Guillaume , Mas Ignacio Suay , Martinez Ramon , Wilcox Rand , Delorme Arnaud 
Published: April 2021

AbstractBeing able to remove or weigh down the influence of outlier data is desirable for any statistical models. While Magnetic and ElectroEncephaloGraphic (MEEG) data are often averaged across trials per condition, it is becoming common practice to use information from all trials to build statistical linear models. Individual trials can, however, have considerable weight and thus bias inferential results (effect sizes as well as thresholded t/F/p maps). Here, rather than looking for univariate outliers, defined independently at each measurement point, we apply the principal component projection (PCP) method at each channel, deriving a single weight per trial at each channel independently. Using both synthetic data and open EEG data, we show (1) that PCP is efficient at detecting a large variety of outlying trials; (2) how PCP-based weights can be implemented in the context of the general linear model (GLM) with accurate control of type 1 family-wise error rate; and (3) that our PCP-based Weighted Least Square (WLS) approach increases the statistical power of group analyses as well as a much slower Iterative Reweighted Least Squares (IRLS), although the weighting scheme is markedly different. Together, our results show that WLS based on PCP weights derived from whole trial profiles is an efficient method to weigh down the influence of outlier EEG data in linear models.Data availabilityall data used are publicly available (CC0), all code (simulations and data analyzes) is also available online in the LIMO MEEG GitHub repository (MIT license).
Developmental diversity and unique sensitivity to injury of lung endothelial subtypes during a period of rapid postnatal growth
Authors: Zanini Fabio , Che Xibing , Knutsen Carsten , Liu Min , Suresh Nina E. , Domingo-Gonzalez Racquel , Dou Steve H. , Zhang Daoqin , Pryhuber Gloria S. , Jones Robert C. , Quake Stephen R. , Cornfield David N. , Alvira Cristina M. 
Published: April 2021

SummaryAt birth, the lung is still immature, heightening susceptibility to injury but enhancing regenerative capacity. Angiogenesis drives postnatal lung development. Therefore, we profiled the transcriptional ontogeny and sensitivity to injury of pulmonary endothelial cells (EC) during early postnatal life. Although subtype speciation was evident at birth, immature lung EC exhibited transcriptomes distinct from mature counterparts, which progressed dynamically over time. Gradual, temporal changes in aerocyte capillary EC (CAP2), contrasted with more marked alterations in general capillary EC (CAP1) phenotype, including distinct CAP1 present only in the early alveolar lung expressingPeg3, a paternally imprinted transcription factor. Hyperoxia, an injury which impairs angiogenesis, induced both common and unique endothelial gene signatures, dysregulated capillary EC cross-talk, and suppressed CAP1 proliferation while stimulating venous EC proliferation. These data highlight the diversity, transcriptomic evolution, and pleiotropic responses to injury of immature lung EC, possessing broad implications for lung development and injury across the lifespan.
A locomotor neural circuit persists and functions similarly in larvae and adultDrosophila
Authors: Lee Kristen M. , Doe Chris Q. 
Published: April 2021

AbstractIndividual neurons can undergo drastic structural changes, known as neuronal remodeling or structural plasticity. One example of this is in response to hormones, such as during puberty in mammals or metamorphosis in insects. However, in each of these examples it remains unclear whether the remodeled neuron resumes prior patterns of connectivity, and if so, whether the persistent circuits drive similar behaviors. Here, we utilize a well-characterized neural circuit in theDrosophilalarva: the Moonwalking Descending Neuron (MDN) circuit. We previously showed that larval MDN induces backward crawling, and synapses onto the Pair1 interneuron to inhibit forward crawling (Carreira-Rosario et al., 2018). MDN is remodeled during metamorphosis and regulates backward walking in the adult fly. We investigated whether Pair1 is remodeled during metamorphosis and functions within the MDN circuit during adulthood. We assayed morphology and molecular markers to demonstrate that Pair1 is remodeled during metamorphosis and persists in the adult fly. In the adult, optogenetic activation of Pair1 resulted in arrest of forward locomotion, similar to what is observed in larvae. MDN and Pair1 are also synaptic partners in the adult, showing that the MDN-Pair1 interneuron circuit is retained in the adult following hormone-driven pupal remodeling. Thus, the MDN-Pair1 neurons are an interneuronal circuit – i.e. a pair of synaptically connected interneurons – that persists through metamorphosis, taking on new input/output neurons, yet generating similar locomotor behavior at both stages.
Shaping employee green behavior: a multilevel approach with Pygmalion effect
Authors: Mo Ziying , Liu Matthew Tingchi , Wu Peiguan 
Published: April 2021

PurposeThe purpose of this study was to theorize and examine a Pygmalion perspective in how leader and coworker expectations predict in-role and ex-role employee green behavior (EGB).Design/methodology/approachUsing a time-lagged field study, data were collected from a sample of 71 leaders and 340 members to examine the hypothesized relationships with a multilevel model (group level and individual level).FindingsThe results showed that leader green behavior and self-efficacy for EGB (i.e. the Pygmalion process) mediate the relationship between leader expectations and EGB, while self-efficacy mediates the relationship between coworker expectations and EGB. In addition, this study found that the effect of coworker expectations and EGB via self-efficacy is stronger when leaders themselves demonstrate a higher level of green behavior.Originality/valueThis study also aims to provide a multilevel theory and investigates the interplay between multilevel variables in encouraging EGB. It also extends previous EGB literature through investigating a different process (i.e. the Pygmalion process) relating leader expectations for EGB to EGB. Moreover, this study develops implications of Pygmalion process on EGB from theoretical and practical perspectives.
The influence of trust and relationship commitment to vloggers on viewers' purchase intention
Authors: Chen Yangyang , Tingchi Liu Matthew , Liu Yongdan , Chang Angela Wen-yu , Yen Jerome 
Published: April 2021

PurposeThis study extends the commitment-trust theory from the perspective of relationship marketing and explores its effect on purchase intention under the moderation of trust by investigating vloggers' relationship marketing in the context of social media.Design/methodology/approachThe study employs a survey investigation with online questionnaires in China, and the hypotheses were tested using multiple regression analyses, with 319 valid consumer responses.FindingsThe findings reveal that the extended commitment-trust theory is applicable in the context of social media. Perceived relationship commitment, expertise, physical attractiveness, social attractiveness and self-disclosure play a significant role in predicting purchase intention. Relationship commitment proves to be a mediator between the antecedents and purchase intention. Trust shows a moderating effect on the antecedents and relationship commitment.Originality/valueThe study provides evidence of the importance of the above-mentioned antecedents in influencing viewers' relationship commitment to vloggers in the context of social media. The results contribute to the development of the commitment-trust theory and an understanding of the theory's underlying mechanisms. The result also provides further evidence of the effect of trust on relationship commitment.
The use of video to maximise cooking skills
Authors: Surgenor Dawn , McLaughlin Christopher , McMahon-Beattie Una , Burns Amy 
Published: April 2021

PurposeThe aim of this research is to examine the impact of video-based learning on the cooking skills development of students. More specifically, exploring the first stages in the learning process through embedding declarative knowledge utilising both video content and learner profiles, with the purpose to make teaching practice more effectively and efficiently targeted.Design/methodology/approachA quantitative social experimental approach was employed. The sample consisted of 414 students from three post primary schools in Northern Ireland. Students were randomly allocated into both control and experimental video content groups. All participants were made aware of ethical procedures and the nature of the study.FindingsThrough the application of latent class analysis (LCA), three distinct types of students were classified. Class one (n = 250) students were termed independent learners, class two (n = 88) students were motivated and benefited from video-based learning and class three (n = 52) students demonstrated an inability to apply information because video did not assist in embedding declarative knowledge.Research limitations/implicationsImplications from this research inform content generation for video-based cooking skills.Practical implicationsGiven the unprecedented move towards online teaching in 2020 due to coronavirus disease 2019 (COVID-19) restrictions, there is increasing interest in targeting resources effectively to meet the requirements of all learning groups. This paper fulfils an identified need to study how video impacts on skills development and learning within specific learning typologies.Originality/valueThis research will be of interest to educationalists in promoting a cost-effective resource in line with constructivist values to streamline and meet the needs of individual learners.
Antioxidant activity of vitamin E enhanced by cyclodextrin inclusion complex
Authors: Chen Weishi , Liu Dong , Zhou Lili , Li Qiquan , Wu Dezhi 
Published: April 2021

PurposeThe study aims to find potential method to enhance the stability and antioxidant activity of vitamins.Design/methodology/approachVitamin E (VE) and β-cyclodextrin (CD) were used to prepare the VE-β-CD inclusion complex via a solution method. The yielded VE-β-CD obtained from optimal experimental conditions was characterized. The mechanism of complex formation and the properties of the complex was investigated by steady-state and time-resolved fluorescence techniques. The antioxidant activity of VE-β-CD was evaluated using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and compared with pure VE.FindingsThree experimental factors – reaction temperature (T), molar ratio of VE to β-CD (rm) and inclusion time (t) – were studied for the preparation of VE-β-CD. At experimental conditions of T = 60°C, rm = 1:3 and t = 3 h, a maximum inclusion rate of VE (78.62%) was obtained. The Fourier-transform infrared spectroscopy (FTIR) shows that VE molecule was physically encapsulated by β-CD in the yielded product. Subsequentially, the steady-state fluorescence intensity of VE was found to increase upon encapsulation by β-CD due to the hindrance of fluorescence quenching of VE molecules inside the hydrophobic cavity of β-CD, and an inclusion constant K was determined to equal 3.58 × 103 L/mol from the fluorescence results. The fluorescent observation was confirmed by time-resolved fluorescence experiment as the lifetime of VE increased with an increase in β-CD concentration.Originality/valueThe ability of removing radicals by VE was significantly enhanced after the formation of inclusion complex with β-CD.
Emotional or logical: reason for consumers to buy organic food products
Authors: Jose Heerah , Kuriakose Vijay 
Published: April 2021

PurposeThe purpose of this paper is to understand, among the emotional, practical and logical factors, which factor is more critical while consumers buy organic food products, mostly fruits and vegetables.Design/methodology/approachA self-administered questionnaire survey approach was used to provide a deeper insight into the reasons for consumers to buy organic fruits and vegetables (OF&V). A total of 632 valid questionnaires were obtained, yielding a response rate of 79%.FindingsHealth is a functional/practical factor which consumer expect as a result of consuming organic food products; however, fear towards conventional food products (emotional) is the triggering factor which motivates consumers to buy OF&V. The logical factor such as environmental motive was found insignificant in the current study, Thereby supporting the value theory which posited emotion greater than practical and which in turn greater than logical. However, barriers for consumers to buy OF&V are perceived price and willingness to take effort. Thus by focusing upon fear reducing strategy such as, implementing certification and labelling on OF&V would be a promising strategy.Originality/valueTo the authors' knowledge, no previous studies exist in the organic consumer behaviour research which used the value theory proposed by Mattson (1991) and the study was able to propose that beyond the practical and logical factors, emotional factor has important role while consumer think of buying OF&V.
Enablers to implement sustainable practices in the service only supply chain: a case of an Indian hospital
Authors: Nagariya Ramji , Kumar Divesh , Kumar Ishwar 
Published: April 2021

PurposeThe inclusion of sustainable practices in the service only supply chain (SOSC) is less evident in the literature. The aim of this research is to analyse the enablers of sustainability to be implemented in (SOSC).Design/methodology/approachA rigorous literature review and experts’ outlook are used to identify fifteen key enablers of sustainability to be implemented in the SOSC. These key enablers are analysed by the interpretive structural modelling (ISM) and fuzzy decision making trial and evaluation laboratory (DEMATEL) technique. A case study of an Indian hospital is taken for testing the relevancy of the research.FindingsISM identified the six hierarchical levels of the enablers and MICMAC classified the enablers into four categories based on the driving and dependence power of the enablers. The fuzzy DEMATEL technique categorized the enablers into cause and effect groups. Four enablers were found in the effect group and eleven enablers were identified in the cause group.Practical implicationsThis research will help the managers in identifying the enablers of sustainability to implement in the SOSC and to handle the critical enablers with care.Originality/valueThis is the first of its kind of study which not only identifies the key enablers to achieve sustainability in the SOSC but also derives the hierarchy levels of enablers and categorizes these enablers into cause and effect groups.
Participatory 3D model to promote intergenerational engagement for disaster risk reduction in São Luiz do Paraitinga, Brazil
Authors: Trejo-Rangel Miguel Angel , Marchezini Victor , Rodriguez Daniel Adres , da Silva Oliveira Melissa 
Published: April 2021

PurposeThe objective of this study was to investigate how participatory 3D mapping can promote local intergenerational engagement for disaster risk reduction.Design/methodology/approachThis investigation was carried out in the city of São Luiz do Paraitinga, Brazil, where a low-cost participatory 3D model (P3DM) was used together with secondary methods (semi-structured interviews, round tables, discussions and presentations) to engage three local focus groups (the general public, high school employees and children) to visualize and interpret local hazards, vulnerabilities, capacities and risk mitigation measures.FindingsParticipants played with a 3D model, using it to express their memories about land use changes in the city and to share their knowledge about past disasters with children that have not faced them. They identified the impacts of the previous disasters and came up with proposals of risk mitigation measures, mostly non-structural.Originality/valueWhen applied in a way that allows spontaneous and open public participation, the participatory 3D model can be a type of disaster imagination game that gives voice to oral histories, local knowledge, and which permits the intergenerational engagement for disaster risk reduction.
The main issue working with migrant construction labor: a case study in Thailand
Authors: Kongchasing Nutchapongpol , Sua-iam Gritsada 
Published: April 2021

PurposeThe purpose of this paper is to study and prioritize the problems impacted on construction work together migrant laborers, by using the Delphi technique. The case study is construction work in Bangkok and metropolitan zones, Thailand.Design/methodology/approachThe framework of the perceived issues caused by working with migrant construction labors especially migrant construction laborers from neighboring countries were identified from literature reviews. The issue list was sent to 162 experts seeking for their opinions. Subsequently, a questionnaire was created from 58 items of suitable issue lists according to expert's opinions. The questionnaires were then submitted to 147 respondents from construction contractor companies. Their responses were calculated and prioritized by means of the Delphi techniqueFindingsThe polling data showed its constancy on the second round of survey. There were 34 out of 58 items passed consensus criteria. The issue “Foreman obtained incompetent or inadequate trained migrant labors when relocate them from/to other site or job” ranked 1st in priority ranking with the average score of 4.56. Subproblems were appropriately prioritized according to their mean scores.Practical implicationsThe results of this research were expected to facilitate construction operators in making appropriate decisions and primary solution concerning main issue factors in working with migrant labors, in order to help even more increasing competition efficiency in Thai construction industries.Originality/valueThe research provides a list of main issue occurred in the case study. These outcomes are also expected to provide important information for other case studies on the issue working with migrant construction labor.