bluuebunny/crossref_metadata_2025_split huggingface dataset:
Clinical Proteomics Reveals Vulnerabilities in Noninvasive Breast Ductal Carcinoma and Drives Personalized Treatment Strategies
Authors: Mitsa Georgia , Florianova Livia , Lafleur Josiane , Aguilar-Mahecha Adriana , Zahedi Rene P. , del Rincon Sonia V. , Basik Mark , Borchers Christoph H. , Batist Gerald 
Published: December 2024

Abstract
Ductal carcinoma in situ (DCIS) is the most common type (80%) of noninvasive breast lesions in women. The lack of validated prognostic markers, limited patient numbers, and variable tissue quality have a significant impact on the diagnosis, risk stratification, patient enrollment, and results of clinical studies. In this study, we performed label-free quantitative proteomics on 50 clinical formalin-fixed, paraffin-embedded biopsies, validating 22 putative biomarkers from independent genetic studies. Our comprehensive proteomic phenotyping reveals more than 380 differentially expressed proteins and metabolic vulnerabilities, which can inform new therapeutic strategies for DCIS and invasive ductal carcinoma. Due to the readily druggable nature of proteins and metabolic enzymes or metabolism inhibitors, this study is of high interest for clinical research and the pharmaceutical industry. To further evaluate our findings, and to promote the clinical translation of our study, we developed a highly multiplexed targeted proteomics assay for 90 proteins associated with cancer metabolism, RNA regulation, and signature cancer pathways, such as PI3K/AKT/mTOR and EGFR/RAS/RAF.

Significance:
This study provides real-world evidence for DCIS, a disease for which currently no molecular tools or biomarkers exist, and gives an unbiased, comprehensive, and deep proteomic profile, identifying &gt;380 actionable targets.
Supracricoid Partial Laryngectomy Versus Radiation Therapy for cT3N0M0 Glottic SCC: Outcomes in Candidates for Total Laryngectomy Responding Well to Induction Chemotherapy
Authors: Laccourreye Ollivier , Corbisiero Michaele Francesco , Garcia Dominique , Mirghani Haitham , Giraud Philippe 
Published: December 2024

Objective: To evaluate whether supracricoid partial laryngectomy (SCPL) may be a viable alternative to radiation therapy (RT) for patients with glottic cT3N0M0 squamous cell carcinoma (SCC) who are surgical candidates for total laryngectomy (TL) and respond well to platinum-based induction chemotherapy. Methods: Retrospective case series review of 18 consecutive patients with cT3N0M0 glottic SCC, initially considered surgical candidates only for TL who showed a good response to platinum-based induction chemotherapy, managed at a French university teaching institution with either SCPL (n = 9) or RT (n = 9). The main endpoints were 10-year local control and laryngeal preservation. The secondary endpoints were 10-year survival, causes of death analysis, and univariate analysis of local control and survival. Results: The 10-year actuarial local control, laryngeal preservation, survival rates were 77.8%, 88.9%, and 66.7% after SCPL, respectively, and 72.9%, 87.5%, and 33.3%, after RT without significant statistical differences. In univariate analysis none of the clinical variables under analysis were related to local control and survival. Conclusions: Our data suggest that SCPL may warrant further consideration as a treatment option for glottic cT3N0M0 SCC patients who respond well to platinum-based induction chemotherapy. However, additional prospective research is warranted given the retrospective, nonrandomized nature of the presented case series.
Nerve Complications with Regional Anesthesia in Foot and Ankle Surgery Avoiding the Popliteal Fossa
Authors: Gerwig Austin , Santoso Breanna , George Maximilian , Cheney Nicholas , O'Connor Patrick 
Published: December 2024

Category: Other Introduction/Purpose: Popliteal nerve blocks are often used to aid intraoperative general anesthesia and provide post-operative pain control for many foot and ankle surgeries. Unfortunately, there is conflicting data regarding complication rates from popliteal nerve blocks, specifically when comparing studies from (the) orthopedic and anesthesia literature. Orthopedic literature notes long term effects (foot drop/sensation deficits) whereas anesthesia literature focuses on immediate post-operative pain control. An approach to block more distal nerves and avoid the popliteal fossa altogether may provide equal anesthetic and pain relief with fewer motor and sensory complications. Our study seeks to address such complications by utilizing an alternative anesthetic approach that targets sensory distributions of nerves that may be affected during the procedure while avoiding major motor neve contributions within the popliteal fossa. Methods: We conducted a retrospective chart review between 2019 and 2023, analyzing complications associated with regional anesthesia achieved via a distal ankle block rather than a popliteal nerve block. Two hundred thirty-seven patients who underwent a variety of foot and ankle procedures (e.g., ankle arthroscopy, ankle fracture repair, cavovarus foot reconstruction, hallux valgus arthrodesis, lateral ankle stabilization, calf tendon revision, midfoot fusion, metatarsal arthrodesis) were included in this study. Results: Out of 237 patients who received a distal ankle block, only three patients were found to have superficial peroneal neuropathy confirmed with EMG, yielding a complication rate of 1.3%. Additionally, six patients required post-operative rescue block for pain control. The remaining 234 patients recovered without complications caused by the distal nerve block approach. Conclusion: The low rate of complications, specifically motor complications, from the distal nerve blocks demonstrate a beneficial alternative to the popliteal nerve blocks for various foot and ankle orthopedic surgeries. This finding is important to consider when choosing an appropriate approach to augment intraoperative general anesthesia accompanying foot and ankle surgeries in the outpatient setting.
Osteotomy and Filling for Osteochondral Lesions of the Tibial Plafond: A Novel Morphology Based Surgical Technique
Authors: Rikken Quinten , Hollander Julian , Jari Dahmen Bsc , M.M.J. Kerkhoffs Gino M , Stufkens Sjoerd 
Published: December 2024

Category: Ankle Introduction/Purpose: Symptomatic primary (large) cystic and non-primary osteochondral lesions of the medial and central tibial plafond are challenging entities to treat. These lesions may benefit from a lesion specific treatment approach. In the present manuscript we describe the novel surgical technique by means of an osteotomy of the distal tibia and cancellous bone graft filling. The procedure involves an oblique distal tibia osteotomy which is made directly over the lesion. Hereafter, the defective lesion tissue is curetted and filled with autologous cancellous bone from the ipsilateral iliac crest, and compression by means of refixation of the osteotomy. The procedure provides provide surgeons an alternative option for OLTP. Methods: Indications: Symptomatic primary cystic and non-primary (i.e., failed prior surgical treatment for the OLTP) osteochondral lesions of the medial and central tibial plafond which failed to respond to a minimum 6 months of nonoperative treatment. Results: Surgical Technique: The procedure is started by means of a curved incision over the medial malleolus, and careful dissection until adequate visualization of the medial malleolus and anteromedial ankle joint is achieved. Hereafter, the screws for the fixation of the osteotomy are predrilled. Then, the OLTP is directly approached with an osteotomy using an oscillating saw directly through the lesion. The lesion is thereafter debrided with a curette until healthy bleeding bone is observed. Subsequently, microfracturing may be performed. Then, autologous cancellous bone is harvested from the ipsilateral iliac crest (or from the osteotomy site in small cysts) and the cyst is filled. Finally, the osteotomy is closed and fixated with two diverging 3.5mm cortical lag screws. Conclusion: Postoperative Management: Post-operatively, there is a period of 5-6 weeks in short leg cast non-weight bearing and 5-6 weeks of short leg cast with weightbearing as tolerated. Hereafter, a CT-scan is made to confirm osteotomy union and patients start a personalized rehabilitation under the guidance of a physical therapist.
GsMYB10 encoding a MYB-CC transcription factor enhances the tolerance to acidic aluminum stress in soybean
Authors: Yang Ce , Lu Xiang , Du Dan , Liang Zhongyi , Li Cheng , Hu Kang , Wang Hongjie , Cheng Yanbo , Lian Tengxiang , Nian Hai , Ma Qibin 
Published: December 2024

Abstract
Background
MYB transcription factors (TFs) play crucial roles in the response to diverse abiotic and biotic stress factors in plants. In this study, the GsMYB10 gene encoding a MYB-CC transcription factor was cloned from wild soybean BW69 line. However, there is less report on the aluminum (Al)-tolerant gene in this subfamily.

Results
The GsMYB10 gene was up-regulated by acidic aluminum stress and rich in the roots with a constitutive expression pattern in soybean. It was found that GsMYB10 protein contains the MYB and coiled-coil (CC) domains, localizes in the nucleus and holds transcriptional activity. The analysis of the transgenic phenotype revealed that the taproot length and root fresh weights of the GsMYB10-OE plants were greater than those of the wild type when subjected to AlCl3 treatments. While the accumulation of Al3+ in root tip of GsMYB10 transgenic plants (59.37 ± 3.59 µg/g) significantly reduced compared with that of wild type (80.40 ± 3.16 µg/g) which were shallowly stained by hematoxylin under the treatments of AlCl3. Physiological indexes showed that the proline content significantly increased 39–45% and the malondialdehyde content significantly reduced 37–42% in GsMYB10-OE plants compared with that of wild type. Transcriptomic analysis showed that overexpression of GsMYB10 induced a large number of differentially expressed genes (DEGs) with Al-treatment, which were related to wall modification related genes included PGs (such as Glyma.19g006200, Glyma.05g005800), XTHs (such as Glyma.12g080100, Glyma.12g101800, Glyma.08g093900 and Glyma.13g322500), NRAMPs and ABCs.

Conclusions
In summary, the data presented in this paper indicate that GsMYB10, as a new soybean MYB-CC TF, is a positive regulator and increases the adaptability of soybeans to acidic aluminum stress. The findings will contribute to the understanding of soybean response to acidic aluminum stress.
Experience of primary dental care teams in managing the oral health of oncology patients
Authors: Wemyss Callum , Abdulsalam Ahmed , Beaton Laura , Perry Kirsten , Scott Claire , Stirling Douglas , West Michele 
Published: December 2024

Abstract
Background
Estimates suggest that one in two people will experience cancer in their lifetime. Cancer and the treatment of cancer can have several impacts on oral health. It is therefore important that dental teams are supported in managing this group of patients especially in primary care dental settings, where most of these patients will first present to dental services. The aim of this study was to explore current practice and beliefs about managing patients with, or who have had, cancer in primary dental care settings.

Methods
Online focus groups consisting of dental professionals working in primary care dental settings in Scotland, were conducted. Areas explored included cancer types seen, perceived role, challenges, and areas where further support was desired. Data from focus group transcripts were analysed using thematic analysis.

Results
Four focus groups were conducted with a total of fifteen participants. Themes identified related to the types of cancers seen in primary care dental settings; communication between dental and medical teams; patient experience; mixed healthcare messages; patient engagement with their healthcare; challenges in treatment planning; apprehension about what can safety managed in primary care; and wider system factors influencing the management of patients with, or who have had, cancer. Areas where support exists but further support is desired were also identified.

Conclusions
Challenges appear to exist in the provision of oral healthcare for patients with, or who have had, cancer. This study has indicated several areas where further support could be targeted. The results should be validated by further research.
Endodontic emergency patients’ profile and treatment outcome – a prospective cohort study
Authors: Haug Sivakami Rethnam , Røegh Margrethe , Fristad Inge 
Published: December 2024

Abstract
Background
Toothache is a debilitating condition, often with mild to excruciating pain, swelling, eating difficulties and insomnia. This study aims to delineate the profiles of patients seeking emergency dental care, focusing on the diagnosis, treatment, and outcomes following non-surgical root canal treatment.

Methods
This prospective cohort study was conducted from 2012 to 2021 at the Section for Endodontics, Department of Clinical Dentistry, University of Bergen, Norway. A total of 281 emergency patient forms were analyzed. Data registered included patient demographics, dental history, chief complaints, medications used, diagnostic results, treatments provided and outcome.

Results
A total of 272 patients (272 teeth) were included in the study. Pain was the predominant complaint (98.5%), where only 57.4% of the patients managed to localize pain to a specific tooth. The mean age of patients was 51.2 years with no significant gender differences. The maxillary right first molar (15.4%) was the most frequent tooth needing treatment. The majority of the patients had experienced pain for three days before they attended the emergency appointment. The most frequently used drug for pain management was paracetamol which was stated to have little effect. Teeth that needed endodontic treatment often had restorations rather than caries. The most frequent diagnoses were pulpitis (26.8%) followed by necrotic pulp (25.4%) and previously root filled teeth (22.8%). Root canal treatment was performed on 60% of the teeth and a success rate of 95% was registered at one-year recall.

Conclusions
There was no singular diagnostic cause leading patients to seek an emergency appointment, highlighting the necessity of a thorough diagnostic procedure. Over the counter pain medications have little effect on alleviating dental pain, often resulting in desperate measures of self-medication. 60% of the teeth needing emergency treatment had previous coronal restorations such as fillings or crowns, indicating that conservative treatment does not appear to fully protect against future pulpal disease. The good prognosis of root canal treatment for teeth with acute symptoms supports recommending dentists to attempt root canal treatment rather than opting for tooth extraction.
Indole-3-carbinol alleviates allergic skin inflammation via periostin/thymic stromal lymphopoietin suppression in atopic dermatitis
Authors: Kang Yun-Mi , Kim Hye-Min , Lee Junho , Baek Jong-Suep , Lee Minho , An Hyo-Jin 
Published: December 2024

Abstract
Background
Atopic dermatitis (AD) is a chronic multifactorial inflammatory skin disorder with a complex etiology. Despite its increasing prevalence, treatment of AD is still limited. Indole-3-carbinol (I3C) is found in cruciferous vegetables and is formed when these vegetables are cut, chewed, or cooked; it exerts diverse pharmacological activities.

Methods
HaCaT keratinocytes stimulated with tumor necrosis factor-α and interferon-γ mixture and NC/Nga mice stimulated with 2,4-dinitrochlorobenzen (DNCB) were used for AD models, in vitro and in vivo, respectively.

Results
The results showed that I3C reduced the expression of pro-inflammatory cytokines, thymic stromal lymphopoietin (TSLP), and periostin in in vitro model. Oral administration of I3C alleviated AD-like skin inflammatory symptoms, including serum IgE levels, epidermal thickening, inflammatory cell infiltration, transepidermal water loss, and scratching behavior. Moreover, I3C decreased the expression of TSLP and periostin and recovered the expression of skin barrier proteins by regulating Aryl Hydrocarbon Receptor and inhibiting the mitogen-activated protein kinase and nuclear factor-κB pathways in the skin of DNCB-induced AD mice.

Conclusions
I3C is suggested as a potential therapeutic alternative for the treatment of AD by repressing allergic inflammatory pathways.
SPP1+ macrophages promote head and neck squamous cell carcinoma progression by secreting TNF-α and IL-1β
Authors: Liu Chun , Wu Kun , Li Chuwen , Zhang Zhen , Zhai Peisong , Guo Haiyan , Zhang Jianjun 
Published: December 2024

Abstract
Background
Head and neck squamous cell carcinoma (HNSCC) is a very aggressive disease characterized by a heterogeneous tumor immune microenvironment (TIME). Tumor-associated macrophages (TAMs) constitute the major innate immune population in the TIME where they facilitate crucial regulatory processes that participate in malignant tumor progression. SPP1 + macrophages (SPP1 + Macs) are found in many cancers, but their effects on HNSCC remain unknown. This study aimed to identify and validate the role and function of SPP1 + Macs in the malignant progression of HNSCC.

Methods
In this study, we applied single-cell RNA sequencing (scRNA-seq) analyses of paired tumor and normal tissues from 5 HNSCC patients to identify tumor-specific SPP1 + Macs. RT-qPCR and multiplex immunohistochemical and multiplex immunofluorescence staining were used to verify the presence of SPP1 + Macs in the clinical samples. Gene set variation analysis suggested that SPP1 + Macs were actively involved in cytokine production. Thus, we constructed SPP1-OE macrophages and SPP1-KD macrophages (both differentiated from THP-1 cells), performed a Luminex liquid suspension chip detection assay to detect differential cytokines, and further assessed their biological functions and mechanisms in several HNSCC cell lines and adjacent macrophages. An in vivo experiment was used to verify the function of SPP1 + Macs in HNSCC progression.

Results
The scRNA-seq results revealed that myeloid cells were heterogeneous and strongly correlated with tumor cells in the TIME in HNSCC and identified tumor-specific SPP1 + Macs, which were positively correlated with poor prognosis of HNSCC patients. Gene set variation analysis (GSVA) suggested that SPP1 + Macs were actively involved in cytokine production. Luminex liquid suspension chip detection assay indicated that SPP1 + Mac-derived TNF-α and IL-1β played important roles. Both in vitro and in vivo experiments and the use of VGX-1027, an inhibitor of macrophage-derived TNF-α and IL-1β, confirmed that SPP1 + Mac-derived TNF-α and IL-1β promoted HNSCC progression by supporting tumor cell proliferation and migration. Mechanistically, we found that TNF-α and IL-1β were upregulated due to NF-kappa B signaling pathway activation in SPP1 + Macs. Moreover, SPP1 + Mac-derived TNF-α and IL-1β promoted the expression of OPN in both tumor cells and other adjacent macrophages through different signaling pathways.

Conclusions
SPP1 + Macs increase the secretion of TNF-α and IL-1β via the NF-kappa B pathway to promote HNSCC cell proliferation, and TNF-α and IL-1β in turn upregulate the expression of OPN in tumor cells and macrophages; thus, SPP1 + Macs may be a candidate target through which antitumor efficacy can be enhanced.
Plants used for the management of venereal diseases in sub-Saharan Africa: a systematic review and critical assessment of their research status
Authors: Omogbene Temitope O. , Lawal Ibraheem O. , Amoo Stephen O. , Adam Anne A. , Gebashe Fikisiwe C. , Aremu Adeyemi O. 
Published: December 2024

Abstract
Background
Sub-Saharan Africa faces one of the highest burdens of venereal diseases (VDs) globally. This review aims to critically evaluate the existing literature on the diverse Indigenous knowledge and medicinal plants utilised for treating VDs in sub-Saharan Africa.

Methods
We used the Preferred Reporting Items for Systematic reviews and Meta-Analyses (PRISMA) protocol to guide the execution of the review. Relevant papers from scientific databases and search engines were assessed. The inclusion criteria included literature published from 2008 and May 16, 2024, and assessment of specific predetermined VDs. Medicinal plants based on certain ethnobotanical indices and data were recorded from each literature.

Results
Among the 131 studies included in this review, a total of 20 relevant ethnobotanical reports were identified, with Nigeria and South Africa having the highest contributions (25% each). A high diversity and richness of 445 ethnobotanically valued anti-venereal plants (99 families) from over 872 Indigenous knowledge holders were inventoried. Plants with the highest diversity of use in traditional treatment of VDs are Cassia abbreviata, Ziziphus mucronata, Ximenia caffra, Catharanthus roseus, and Terminalia prunioides. The most represented families are Fabaceae (15.8%), Cucurbitaceae (5.9%), Solanaceae (5.9%), Euphorbiaceae (5%), and Combretaceae (5%). Roots and leaves were highly utilised with frequencies of 41.5% and 26.3%, respectively. The most used method of preparation are decoctions (36.7%) and infusions (12.2%), whereas oral route (72.9%) dominated the mode of administration of the medicinal plants.

Conclusions
This review consolidated data from sub-Saharan Africa—notwithstanding a limited number of studies in quantitative synthesis—and identified a diverse array of ethnobotanically valued anti-venereal plants, enabling meaningful conclusions to be drawn for future ethnopharmacological assessments. Effective plant conservation and advancement of ethnobotanical research in the region require stringent regulations and cross-country collaborations.
Survival and mortality after extrapleural pneumonectomy versus pleurectomy/decortication for malignant pleural mesothelioma: a systematic review
Authors: Elsayed Hany Hasan , Abdel-Gayed Mohammed 
Published: December 2024

Abstract
Background
Surgery for malignant pleural mesothelioma (MPM) has always been a source of debate since no strong evidence exists for performing a radical surgical intervention. We aim to systematically review and compare different macroscopic resection procedures. A systematic Review on PUBMED/MEDLINE, EMBASE, and COCHRANE LIBRARY from Inception until March 2024 was performed to search for all studies comparing the 30-day mortality and overall survival after extrapleural pneumonectomy (EPP) and Pleurectomy decortication or extended Pleurectomy decortication (PD/EPD). We excluded studies with less than 10 patients, case reports, and articles with no endpoints.

Main body
Our electronic search retrieved 18,124 citations of which 22 studies fulfilled our criteria. The median 30-day mortality for PD/EPD was 2.2% (range 0–4%) while the median overall survival was 21 months (range 10.4–36 months) while EPP has a median 30-day mortality of 6% (range 0–11.8%) with a median overall survival of 18.1 months (range 9.3–35 months).

Conclusion
Surgery for MPM should be for selected patients and performed in specialized centers after discussions in multidisciplinary team meetings. PD/EPD seems to be associated with a lower 30-day mortality rate and a superior overall survival and should be considered the first radical option if surgery is deemed appropriate for patients.