bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Prognostic Nomogram Combining Gene Expression Profiles and TNM Stage Predicts Survival in Gastric Cancer
Authors: Huang Jian , Wang Dongcun , Wang Xiaoliang , Ye Xiaoxing , Da Jiping 
Published: August 2021

Abstract
BackgroundGastric carcinoma (GC) is a highly aggressive malignancy and is associated with high morbidity and mortality rates around the world, the current tumor-node-metastasis (TNM) staging system is inadequate to predict overall survival (OS) in GC patients. therefore, potential forecasting methods for prognosis are important to investigate.MethodsDifferentially expressed genes (DEGs) were screened using gene expression data from The Cancer Genome Atlas (TCGA). We then construct a risk score signature model by univariate Cox proportional hazards regression (CPHR) analysis, the Kaplan-Meier method（KM）and multivariate CPHR analysis. Using TNM stage, we developed a signature-based nomogram. Finally, we utilize an independent Gene Expression Omnibus dataset (GSE62254) validate the prognostic value of risk score signature model and nomogram.ResultsWe identified five OS-related mRNAs among 1113 mRNAs that were differentially expressed between GC and normal samples in the TCGA dataset. We then constructed a five-mRNA signature model, which efficiently distinguished high-risk from low-risk patient in both cohort, and even viable in the TNM stage-III, gender(male, female) and age(＜65-year-old, ≥65-year-old) subgroups (P<0.05). Utilizing TNM stage, we developed a signature-based nomogram, which performed better than use the TNM stage or five-mRNA signature alone for prognostic prediction in the TCGA and GSE62254 dataset.Conclusions﻿These results suggest that both risk signature and nomogram were effective prognostic indicators for patients with GCs, and could potentially be used for individualized management of such patients.
Evaluate the Effectiveness of Hubbard Purification Rundown Process for Victims of Agent Orange/Dioxin, Related Strengths and Challenges
Authors: Hoang Hau Duc , Tran Tam Ngoc 
Published: August 2021

Abstract
Objective: The aim of this study was to assess the effectiveness of the Hubbard purification rundown (PR) process for victims of Agent Orange (AO)/dioxin at detoxification centers. Methods: The study was designed as a cross-sectional study combining quantitative and qualitative methods and carried out on 30 dioxin patients, 21 health workers, and 299 medical records in Hanoi and Da Nang centers for dioxin detoxification (CDD) of Vietnam.Results: A small number of patients were clinically examined before enrolling in and after finishing the treatment, 35% and 0%, respectively. In addition, 15% of patients did not finish their daily PR session in the second step, while 20% of them were not evaluated daily for treatment effectiveness in the fourth step by health workers. Furthermore, 20% of patients did not follow all the 6 steps of the Hubbard PR program. Although centers were sufficiently equipped in terms of infrastructure and equipment, the study showed that there remained barriers in implementing the Hubbard PR process, such as the lack of human resources, wasteful usage of equipment, and the lack of technology application for electronic medical records management. For patients, the difficulties include the lack of awareness and understanding regarding the program, and high temperature during the PR sessions (63%), long PR duration (47%), too many medications and supplements (37%), as well as high cost of treatment (35%). Conclusion: Ensuring adherence and compliance at all steps in the Hubbard PR process can have a positive impact on the health improvement of AO/dioxin victims.
An Integrated Analysis Reveals Geniposide Extracted From Gardenia Jasminoides Ellis Regulates Calcium Signaling Pathway Essential for Influenza a Virus Replication
Authors: Zhou LiRun , Bao Lei , Wang Yaxin , Chen Mengping , Zhang Yingying , Geng Zihan , Zhao Ronghua , Sun Jing , Bao Yanyan , Shi Yujing , Yao Rongmei , Guo Shanshan , Cui Xiaolan 
Published: August 2021

Abstract
BackgroundGeniposide, an iridoid glycoside purified from the fruit of Gardenia jasminoides Ellis, has been reported to possess pleiotropic activity against different diseases. In particular, geniposide possesses a variety of biological activities and exerts good therapeutic effects in the treatment of several strains of the influenza virus. However, the molecular mechanism for the therapeutic effect has not been well defined.MethodsThis study aimed to investigate the mechanism of geniposide on influenza A virus (IAV). The potential targets and signaling pathways of geniposide in the IAV infection were predicted using network pharmacology analysis. According to the result of network pharmacology analysis, we validated the calcium signaling pathway induced by IAV and investigated the effect of geniposide extracted from Gardenia jasminoides Ellis on this pathway. ResultsThe primary gene ontology (GO) biological processes and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways KEGG enrichment analysis indicated that geniposide has a multi-target and multi-pathway inhibitory effect against influenza, and one of the mechanisms involves calcium signaling pathway. In the current study, geniposide treatment greatly decreased the levels of RNA polymerase in HEK-293T cells infected with IAV. knocking down CAMKII in IAV-infected HEK-293T cells enhanced virusRNA (vRNA) production. Geniposide treatment increased CAMKII expression after IAV infection. Meanwhile, the CREB and c-Fos expressions were inhibited by geniposide after IAV infection. The experimental validation data showed that the geniposide were able to alleviated extracellular Ca2+ influx, dramatically decreased neuraminidase activity and suppressed IAV replication in vitro via regulating the calcium signaling pathway. ConclusionsThese anti-IAV effects might be related to the disrupted interplay between IAV RNA polymerase and CAMKII and the regulation of the downstream calcium signaling pathway essential for IAV replication. Taken together, the findings reveal a new facet of the mechanism by which geniposide fights IAV in a way that depends on CAMKII replication.
Home Intervention for Children with Unilateral Upper Limb Reduction Deficiencies
Authors: Lukaszek Jessica , Borrell Jordan , Cortes Claudia , Zuniga Jorge 
Published: August 2021

Abstract
Purpose: Current training interventions assessing pediatric functional motor skills do not account for children with upper limb reductions who utilize a prosthetic device. It was hypothesized that a newly created 8-week Home Intervention program will result in significant improvements in gross manual dexterity, bimanual coordination, and the functional activities performed during the program. It was also hypothesized that the newly developed Prosthesis Measurement of Independent Function (PMIF) score will reflect the Home Intervention performance improvements. Methods: Five pediatric participants (ages 5-19 years) with congenital upper limb reductions were fitted with a 3D printed upper extremity prosthesis for their affected limb. Participants then completed the 8-week Home Intervention which included Training activities completed 2x/week for 8 weeks and Non-Training activities completed only at week 1 and week 8. Participant’s times were recorded along with each participant receiving a PMIF score ranging from 0 = unable to complete activity, to 7 = complete independence with activity completion. Results: Results showed a decrease in overall averaged activity times amongst all activities. For all activities performed, individual averaged time decreased with the exception of Ball Play which increased over the 8 week intervention period. There was significant interaction for home intervention performance with F = 2.904 (p = 0.003). All participants increased their PMIF scores to 7 (complete independence) at the end of the 8 week intervention period. Conclusion: Decreases in time averages and increases in PMIF scores quantitatively showed that the home intervention program facilitate improvements in function and independence.
Microstructural Studies and Material Characterization of Alumina Nanoparticulate Reinforced Functionally Graded Al-12Si (wt.%) alloy, produced using Centrifuge Casting Technique
Authors: KS Chethan , S Kiran Aithal , HN Manjunath , N Ramesh Babu , KN Pavan 
Published: August 2021

Abstract
The current research work discusses the concept of reinforcing alumina nanoparticulate to Al-12Si (wt.%) alloy using a unique casting technique known as centrifuge casting system established in-house at our advanced manufacturing centre. The resulting cast solid cylindrical specimen will be functionally graded along the length of the specimen containing higher percentage of primary silicon and alumina nanoparticulate at the top region than the bottom region, with a gradual transition in between the two regions. The microstructure of the functionally graded Al-12Si (wt.%) alloy, reinforced with 0.5, 1 and 1.5wt.% of alumina nanoparticulate was analysed using “Scanning Electron Microscope (SEM)” and the elemental composition of the cast specimens were obtained using “Energy-dispersive X-ray analysis (EDX)”. The outcomes of the EDX analysis confirmed the presence of alumina nanoparticulate in the cast specimens. Further, the mechanical properties of the cast Al-12Si (wt.%) alloy, reinforced with alumina nanoparticulate were studied, and it was found that the specimen with the addition of alumina nanoparticulate showed enhanced mechanical properties when compared with the Al-12Si (wt.%) alloy, without alumina nanoparticulate addition, cast under identical conditions.
FD-895 and Pladienolide B Modulate RNA Splicing Associated With Wnt Signaling Pathway
Authors: Kumar Deepak , Kashyap Manoj K. , Yu Zhe , Spaanderman Ide , Villa Reymundo , Kipps Thomas J. , Clair James J. La , Burkart Michael D. , Castro Januario E. 
Published: August 2021

Abstract
BackgroundAlterations in RNA splicing are associated with different malignancies including leukemia, lymphoma, and solid tumors. The RNA splicing modulators such as FD-895 and pladienolide B have been investigated in different malignancies to target/modulate spliceosome for therapeutic purpose. MethodsThe different cell lines were screened using RNA splicing modulator to test in vitro cytotoxicity as well as ability to modulate RNA splicing capability via induction of intron retention (using RT-PCR and qPCR) was assessed. The Cignal Finder Reporter Array was used to screen the HeLa cell line to know the pathway affected the most by the splice modulators. Further the candidates associated with the pathways were validated at protein levels using western blot assay and gene-gene interaction study were carried out using GeneMANIA.ResultsWe show that FD-895 and pladienolide B have more apoptotic activities than conventional chemotherapy in different solid tumors. We found FD-895 or pladienolide B modulate Wnt signaling pathways and mRNA splicing. We showed that FD-895 or pladienolide B significantly down regulates not only Wnt signaling pathway-associated transcripts (GSK3β and LRP5), but also both transcript and proteins including LEF1, CCND1, LRP6, and pLRP6. ConclusionThese results indicate FD-895 and pladienolide B inhibit Wnt signaling by impeding with phosphorylation of LRP6 and modulates mRNA splicing through induction of intron retention in cervical cancer cells.
Chemopreventive and Therapeutic Efficacy of Enhalus Acoroides Against Diethylnitrosamine Induced Hepatocellular Carcinoma in Wistar Albino Rats
Authors: Parthasarathy Amudha , VIDYA R. 
Published: August 2021

Abstract
Background: Hepatocellular carcinoma plays an inadequate mention in the second cause of death because of cancer worldwide. An alternative therapy with high rate of prognosis and also without side effects. Several data indicated the therapeutic efficacy of Enhalus acoroides. There were no scientific studies on chemopreventive and antioxidant potential of Enhalus acoroides against hepatocellular carcinoma.Purpose: To investigate the hepatoprotective efficacy of ethanolic extract of Enhalus acoroides (EEEA) against DEN induced hepatocellular carcinoma using wistar albino rats.Study design: Animals were divided into five groups each comprising six rats. Normal saline given to Group I- Control rats. By using DEN, liver cancer was induced to Group II, III, IV and V rats as single intraperitoneally injection (100 mg/kg body weight). At the beginning of 6th week, Groups III rats received EEEA (200mg/kg body weight/day) upto 16 weeks. Group IV rats received EEEA for one week before the administration of DEN and continued till the 16th week. After the administration of DEN, Group V positive control rats received Silymarin (100 mg/kg body weight) at the beginning of 6th week and continued upto 16 weeks. The efficacy of Enhalus acoroides for its Hepatoprotective and antioxidant properties during its simultaneous treatment against DEN induced liver damage was evaluated in rats. Methods: The hepatoprotective efficacy of EEEA (200 mg/kg) was investigated against DEN (100 mg/kg/b.w) induced hepatotoxicity, was measured by evaluating serum liver markers levels (ALT, AST, GGT and ALP), Kidney markers (Urea and Creatinine), Lipid profile (TG, HDL, LDL & Total cholesterol) and Serum tumor markers (DNA, RNA, AFP and CEA). EEEA-aided antioxidant defence against hepatotoxic insult of DEN was measured by evaluating various Antioxidant biomarkers (GSH, SOD, CAT, GPx, Vit C and Vit E) Morphometric gross analysis and Histopathological studies were done to support the outcomes of the present study.Results: A significant increased antioxidant defence and reduced MDA levels in the serum of EEEA treated animals compared to the DEN induced animals. The resulting data showed that the administration of EEEA decreased the serum liver markers levels, kidney markers, Lipid profile and serum tumor markers when compared to the untreated rats. The histopathological anomalies were altered on administration of EEEA indicating its protective effects on hepatocytes when compared with untreated rats.Conclusions: Our consequences established that crude ethanolic extract of Enhalus acoroides shown an effective impact against DEN-induced hepatocellular carcinoma, and serves as a better option for chemopreventive treatments.
Genetic Variability of Acetolactate Synthase (ALS) Sequence in Centaurea Cyanus Plants Resistant and Susceptible to Tribenuron-methyl
Authors: Wrzesinska Barbara , Praczyk Tadeusz , Obrępalska-Stęplowska Aleksandra 
Published: August 2021

Abstract
Centaurea cyanus, belonging to the Asteraceae family, is an arable weed species being encountered mainly in the fields with cereals, sugar beet, and corn. C. cyanus high genetic variability has recently been reported, however, little is known about sequence variability in the context of herbicide resistance. C. cyanus resistance was found mainly against acetolactate inhibitors (ALS) inhibitors, but no ALS sequence information concerning herbicide resistance mechanism has been published yet. Therefore, the aim of this study was to determine ALS sequences for biotypes susceptible and resistant to tribenuron-methyl in order to identify possible mutations conferring the resistance. DNA isolation from susceptible and resistant plants was followed by PCR amplification and sequencing of ALS sequence. As a result different lengths of DNA products were obtained. Moreover, both nucleotide and amino acid sequence analysis revealed high sequence variability within one plant as well as between plants from the same biotype. In a few resistant plants, six changes in amino acid sequence were identified in comparison to susceptible ones. However, these preliminary studies require further investigation toward confirming the significance of these mutations in herbicide resistance development. This study provides the first attempt in the research on C. cyanus target-site resistance mechanism.
Addition of Camrelizumab to Transarterial Chemoembolization in Hepatocellular Carcinoma With Untreatable Progression
Authors: Ren Yanqiao , Liu Ziyi , Makamure Joyman , Kan Xuefeng , Song Songlin , Liu Yiming , Qian Kun , Zheng Chuansheng , Liang Bin 
Published: August 2021

Abstract
Purpose: To evaluate the efficacy and safety of camrelizumab addition to transarterial chemoembolization (TACE) in treatment of hepatocellular carcinoma (HCC) with TACE-related untreatable progression (UP).Methods: Patients with HCC who received addition of camrelizumab due to UP after initial TACE treatment were enrolled at our institution between May 2019 and January 2021. Patients were assessed for tumor response, progression-free survival (PFS), and adverse events. Risk factors for PFS were evaluated with logistic regression analysis.Results: A total of 41 patients were included. The objective response rate and disease control rate were 24.4% and 61.0% at 2-3 months, and 12.2% and 58.5% at 6 months, respectively. The median PFS of the patients were 6 months (95% CI: 3.8 months, 8.2 months). Of the 41 patients, 23 patients received camrelizumab combined with TACE (hereafter, camrelizumab-TACE), in whom 52 combined TACE procedures were performed, with a median of 2 procedures (range, 1-6) per patient. The remaining 18 patients received camrelizumab alone due to TACE contraindications. Multivariable analysis indicated that camrelizumab-TACE was an independent prognostic factor for PFS. Subgroup analysis showed a median PFS of 8 months in the camrelizumab-TACE group and 3 months in the camrelizumab monotherapy group (P<0.001). No treatment-related mortalities occurred. Seventeen patients (41.5%) developed at least one type of adverse events after treatment with camrelizumab, with reactive cutaneous capillary endothelial proliferation (n=14, 34.1%) being the most common adverse events.Conclusions: Addition of camrelizumab to TACE offered an effective and safe treatment for HCC with UP.
The Prognostic and Predictive Value of Pretreatment Serum Tumor Markers in Lung Adenocarcinoma with Different EGFR Status
Authors: Chen Qian , Li YanLan , Feng Yan , Li Xuemei , Jian Dan , Luo Hao , Qian Chengyuan , Zhu JianWu , Wang Dong , Dai Nan , Li mengxia 
Published: August 2021

Abstract
Background: The prognostic value of carcinoembryonic antigen (CEA), cytokeratin-19 fragments (Cyfra21-1), neuron-specific enolase (NSE), Glycogen Antigen 153, Glycogen Antigen 199 has been investigated in Lung Adenocarcinoma (LUAD). However, few studies have directly focused on the association between serum tumor markers and epidermal growth factor receptor (EGFR) mutation status.Material and Method: 146 patients with stage IIIB and IV LUAD were enrolled between 2008 and 2018. Correlations between serum tumor marker levels, EGFR mutations and survival were analyzed and prognostic factors were identified.Result: Of eligible 90 patients with EGFR-mutant LUAD, CA 15-3 (7 versus 9 months, 0.037 for PFS; 25 months versus 36 month, P = 0.003 for OS, ) and Cyfra21-1 (7.0 versus 9.0 months, P =0.010 for PFS, 25 months versus 36 months, P=0.044 for OS,showed negatively significant correlation with overall survival and Progression free survival. For parents without EGFR-mutation, CA125 (month versus 8 months, P = 0.013 for DFS, 18 months versus 24 month, P = 0.016 for OS) also showed negatively significant correlation with overall survival and Progression free survival.Conclusion: CA153 and Cyfra21-1 was a prognostic serum biomarker in EGFR-mutant LUAD patient, coincide with CA125 in EGFR-WT LUAD group.Cyfra21-1 levels was also a predictive serum biomarker for in EGFR-mutant LUAD patient who received EGFR-TKI treatment.
Quantitative and visualized analysis of 5,097 triple-negative breast cancer (TNBC) publications by using multiple machine learning algorithms: TNBC is an excellent forerunner in cancer research
Authors: Wang Kangtao , Zheng Chanjuan , Xue Lian , Deng Dexin , Tan Fengbo , Pei Qian , Li Yuqiang , Wang Dan , Zeng Liang , Li Ming , Deng Xiyun 
Published: August 2021

Abstract
Background:

Triple-negative breast cancer (TNBC) is a subtype of breast cancer proposed at the beginning of this century, which is still the most challenging breast cancer subtype due to its aggressive behavior, including early relapse, metastatic spread, and poor survival. This study explores current research status and deficiencies from a macro perspective on TNBC publications by using machine learning methods.
Methods:

All publications under the MeSH term "Triple Negative Breast Neoplasms" in PubMed were searched and downloaded as of December 2020. R and Python were used to extract MeSH terms, geographic information and other abstracts from metadata. The Latent Dirichlet Allocation algorithm was applied to identify specific research topics. The Louvain algorithm was used to establish a topic network, identifying the relationship between the topics.
Results:

A total of 5,097 publications were identified, with an average annual growth rate of 33.5%. Only 88 countries and regions in the world participated in TNBC research. The publications contain about 25% of clinical trial-related research. The rapid clinical progress will provide an excellent example for diagnosing and treating other tumors, suggesting that TNBC is an excellent forerunner in cancer research. Publications of pathogenic mechanisms and drugs are most studied. Using the Topic model, we found that the publications were mainly focused on three aspects: treatment plan research, new biomarkers research, and the regulation mechanism for TNBC aggressive behavior.
Conclusion:

This study quantitively summarizes the current status of TNBC research from a macro perspective and will aid in redirecting basic and clinical research toward a better outcome of TNBC.
LukS-PV Induced HOXA9 Degradation in Acute Myeloid Leukemia Cells via Autophagy
Authors: Qiang Ping , Fang Chao , Song Kaidi , Shi Lan , Dai Yuanyuan , Chang Wenjiao , Xu Liangfei , Ma Xiaoling 
Published: August 2021

Abstract
Aberrant over-expression of HOXA9 is a prominent feature of AML driven by multiple oncogenes, thus therapeutic degradation of HOXA9 by autophagy may be an effective treatment strategy for AML. PVL is a pore forming cytotoxin secreted by Staphylococcus aureus, and it is composed of two subunits - LukS-PV and LukF-PV. Here, we show that LukS-PV can stimulate the conversion of LC3-I to LC3-II in AML cells in a concentration-dependent manner, and autophagic vacuoles can be found in LukS-PV-treated THP-1 cells. Furthermore, we find that an accumulation of LC3-positive structures in AML cells exposed to LukS-PV, indicating that an increased autophagic flux were formed. Therefore, LukS-PV induced autophagy of AML cells. We also demonstrated that LukS-PV could regulate the expression of HOXA9 at the protein level. HOXA9 molecules were detected in autophagosomes after LukS-PV treatment, indicating that autophagy induction accounted for the degradation of HOXA9.
Localized waves and mixed interaction solutions with dynamical analysis to the Gross-Pitaevskii equation in the Bose-Einstein condensate
Authors: Wang Haotian , Zhou Qin , Biswas Anjan , Liu Wenjun 
Published: August 2021

Abstract
We report a kind of breather, rogue wave and mixed interaction structures on a variational background height in the Gross-Pitaevskii equation in the Bose-Einstein condensate by the generalized Darboux transformation method, and the effects of related parameters on rogue wave structures are discussed. Numerical simulation can discuss the dynamics and stability of these solutions. We numerically confirm that these are correct, and can be reproduced from a deterministic initial profile. Results show that rogue waves and mixed interaction solutions can evolve with a small amplitude perturbation under the initial profile conditions, but breathers cannot. Therefore, these can be used to anticipate the feasibility of their experimental observation.