bluuebunny/crossref_metadata_2025_split huggingface dataset:
Genome-wide Association Study for Grain Mineral Content in a Brazilian Common Bean Diversity Panel
Authors: Delfini Jessica , Moda-Cirino Vânia , Neto José dos Santos , Zeffa Douglas Mariani , Nogueira Alison Fernando , Ribeiro Luriam Aparecida Brandão , Ruas Paulo Mauricio , Gepts Paul , Gonçalves Leandro Simões Azeredo 
Published: March 2021

Abstract
Biofortification is one of the strategies developed to address malnutrition in developing countries, the aim of which is to improve the nutritional content of crops. The common bean (Phaseolus vulgaris L.), a staple food in several African and Latin American countries, has excellent nutritional attributes and is considered a strong candidate for biofortification. The objective of this study was to identify genomic regions associated with nutritional content in common bean grains using 178 Mesoamerican accessions belonging to a Brazilian Diversity Panel (BDP) and 25,011 good-quality single-nucleotide polymorphisms. The BDP was phenotyped in three environments for nine nutrients (phosphorus, potassium, calcium, magnesium, copper, manganese, sulfur, zinc, and iron) using four genome-wide association multi-locus methods. To obtain more accurate results, only Quantitative-Trait Nucleotides (QTNs) that showed repeatability (i.e., those detected at least twice using different methods or environments) were considered. Forty-eight QTNs detected for the nine minerals showed repeatability and were considered reliable. Pleiotropic QTNs and overlapping genomic regions surrounding the QTNs were identified, demonstrating the possible association between the deposition mechanisms of different nutrients in grains. The accumulation of favorable alleles in the same accession was associated with a gradually increasing nutrient content in the grain. The BDP proved to be a valuable source for association studies. The investigation of different methods and environments showed the reliability of markers associated with minerals. The loci identified in this study will potentially contribute to the improvement of Mesoamerican common beans, particularly carioca and black beans, the main groups consumed in Brazil.
A Privacy-Preservation Scheme based on Mobile Terminals in Internet Medical
Authors: Gao Shuo , Wang Qichao , Liu Yihan , Liu Zekai , Ou Wei , Han Wenbao 
Published: March 2021

Abstract
Since the 21st century, Internet technologies have entered a stage of rapid developments, and gradually been widely used in various industries. Internet Medical is gradually matured and used widely. Aiming at the problems such as the easy leakage of privacy in mobile medical equipment and untrustworthy data, we make use of a role-separated mechanism to generate trusted anonymous certificates and propose a lightweight identity authentication scheme to protect the medical data security. It reduces the calculation cost. Meanwhile, in view of problems of transparency and visibility of blockchain information, we adapt searchable encryption algorithm to realize ciphertext processing in the whole life cycle. Experiments show that our scheme can reduce the cost of computation on basis of ensuring traffic. In process of dynamic updating of ciphertext keywords, except the keyword identifier, less information is leaked to the server, which protect users’ privacy.
Fuzzified Swarm Intelligence Framework Using Fpsor Algorithm for High-Speed Manet
Authors: Gopalan Harihara 
Published: March 2021

Abstract
Ad-hoc routing protocols may be dispersed and involve every node in the route discovery process by making routing data more reliable. Mobile-Adhoc-Networks (MANETs) consist of many portable nodes that can commune directly with each other or through intermediate nodes. Repeatedly, nodes in MANETs operate with batteries and can roam freely, and thus, a node may exhaust its energy or move away without providing any notice to its cooperative nodes. In MANETs, a route consists of numerous links in sequence, and so, its lifetime is based on the lifetime of every node and also the wireless links among adjacent nodes. In this research work Fuzzified Particle Swarm Optimization oriented Routing (FPSOR) algorithm is planned to minimize data loss and computational overhead, which in turn maximizes the lifetime of MANETs. Particle Swarm Optimization oriented Routing protocol (PSOR) has taken energy competence as a significant criterion for processing routing and driving optimised path for the data accelerating and processing to the source node. The PSOR produces a fresh route for routing by considering the fitness value of energy to evaluate diverse paths and to choose the best-optimised path whose energy consumption is less as compared to ant colony optimization routing paths. This algorithm utilizes a good approach focusing energy levels/status of the nodes through fuzzification and the lengths of the routed ways. NS2 simulator is cast for performance evaluation.
The Fingerprint of Indian Ocean Rain and River Water in Salinity and Circulation
Authors: Valsala Vinu 
Published: March 2021

Abstract
Per unit area of the tropical Indian Ocean receives the world’s largest tropical ocean rain and river runoff (RRW). The 3-dimensional spreading of RRW entering the tropical Indian Ocean and associated salinity and circulation anomalies are explored for 60 years using ocean reanalysis data tailored to a tracer transport model. Over 60 years, the cumulative impact of RRW entering the tropical Indian Ocean is to freshen the Indian Ocean basin as large as 2-0.1 p.s.u from the surface to 500m. The RRW has propagated to a vast extent of the Atlantic and Pacific Oceans via general circulation pathways. A quasi-equilibrium model of accumulation of RRW over the tropical Indian Ocean suggests that it induces clockwise geostrophic currents from the Bay of Bengal to the Arabian Sea over 0-500m depths, a net inter-basin transport tendency of 0.8±0.14 Sv year-1. The study implies that coupled climate models with apparent precipitation biases may miscalculate such salinity and circulation anomalies due to RRW and aggravating biases in simulated climate dynamics.
Decompression Surgery With Antibiotic-impregnated Calcium Sulfate Can Achieve More Satisfactory Results Than Decompression Alone in Pediatric Hematogenous Osteomyelitis
Authors: Qin Cheng-he , Tao Rui , Chee Yu-han , Luo Ji-wei , Xu Lei , Fang Jia , Hu Yan-jun , Zhou Chun-hao 
Published: March 2021

Abstract
BackgroundThe curative effect of antibiotic-impregnated Calcium Sulfate in pediatric ostemyelitis is unknown. The purpose of this study was to compare the outcomes of two treatment methods, decompression combined with antibiotic-impregnated calcium sulfate and decompression alone, for the treatment of pediatric Hemotogenous Osteomyelitis.MethodsBetween 2013 to 2016, forty-one patients with Hematogenous Osteomyelitis met the criteria were included for assessment. Twenty-one patients were included in the calcium sulfate group (the CS group) in which vancomycin and/or gentamicin impregnated calcium sulfate was used as an adjuvant after bone decompression while 20 patients as the control group were undergone bone decompression alone. The infection recurrence rate, hospital stay, wound healing duration, serum inflammatory index, pathological fracture, leakage of the incision ,follow-up time were compared between the two groups.ResultsInfection recurrence was 0% (0/21) in CS group and 15%(3/20) in control group within 12 months(P=0.107). Infection recurrence was 0%(21/21) in CS group and 20% (16/20) of patients at a minimum of 24(range 24 to 67) months follow-up(P=0.048). Mean hospital stay were 8.19(range 3 to 21) days in CS group and 15.95(range 5 to 47) days in control group(P=0.02). Mean wound healing duration were 16.1(range 10 to 29) days in CS group and 15.5(range 10 to 25) days in control group(P=0.65). Serum inflammatory index for WBC(White Blood Cells), ESR( Sedimentation Rate), CRP(C-Reactive Protein) was respectively 8.76(6.03-12.07)*10^9/L, 44.14(26-70)mm/L, 12.35(1.03-35.04) mg/Lin CS group and 8.90(5.68-13.56)*10^9/L, 39.25(19-57)mm/L, 15.65(1.02-45.69) mg/L in control group(P=0.82, P=0.31, P=0.51). Pathological fracture was 0% (0/21) in CS group and 10%(2/20) in control group(P=0.23). Leakage of the incision was 4.76% (0/21) in CS group and 10%(2/20) in control group(P=0.61). ConclusionsAntibiotic-impregnated Calcium Sulfate can reduce infection recurrence and shorten the mean hospital stay in pediatric Hemotogenous Osteomyelitis. The curative effect of antibiotic-impregnated Calcium Sulfate in pediatric ostemyelitis is satisfactory. Trial registrationRetrospectively registered.
Abscisic Acid Metabolism in Leaves and Roots of Four Vitis Species in Response to Water Deficit
Authors: Toups Haley S. , Cochetel Noe , Galdamez Kevin , Deluc Laurent , Cramer Grant R. 
Published: March 2021

Abstract
Background: Abscisic acid is a phytohormone involved in water deficit response. Abscisic acid metabolism is regulated by biosynthesis, conjugation, and catabolism. NCED3 is the rate limiting step of abscisic acid biosynthesis and is a key contributor to plant water deficit responses. In this study NCED3 transcript accumulation and abscisic acid metabolism were further characterized as key water deficit responses in four Vitis species (Vitis vinifera (Cabernet Sauvignon), Vitis champinii (Ramsey), Vitis riparia (Riparia Gloire), and Vitis vinifera x Vitis girdiana (SC2)) under three levels of water deficit in leaves and roots. Results: The concentrations of abscisic acid and derivative metabolites increased with water deficit and was dependent upon the species. RNA-Seq and RT-qPCR data were consistent with the changes in abscisic acid metabolite concentrations; the corresponding transcript abundances substantiate NCED3 as a key gene in the water deficit response; however, NCED3 protein concentrations assayed in Western Blots were not affected. Major differences in abscisic acid metabolism at the gene, protein, and metabolite levels were detected between leaves and roots in these four species. NCED3 transcript abundance and abscisic acid concentration in drought-tolerant Ramsey increased earlier and more significantly than the other species during long-term, moderate to severe water deficits but were not stimulated as much by short-term, rapid dehydration. In drought-sensitive Riparia, NCED3 transcript abundance and abscisic acid metabolite concentrations increased to a lower extent than in Ramsey during moderate to severe water deficits, but short-term rapid dehydration induced a significantly higher abscisic acid concentration in Riparia than Ramsey. Conclusions: Grapevine species have distinct abscisic acid metabolism that depends highly on the severity and duration of stress and organ (leaves or roots). This study confirms that abscisic acid metabolism and NCED3 are part of a core water deficit response in Vitis species. Relative quantities of transcripts, proteins, abscisic acid and derivative metabolites were determined, but many aspects of abscisic acid metabolism and water deficit responses warrant additional investigation. This study provides a better understanding of how Vitis is adapted to dry environments, which may be exploited for future breeding programs.
Exploring Cardioprotective Potential of Esculetin Against Isoproterenol Induced Myocardial Infarction in Rats: In Vivo and in Vitro Evidence
Authors: P Pullaiah Chitikela , Nelson Vinod K , R Sushma , GV Narasimha Kumar , K Thyagaraju 
Published: March 2021

Abstract
BackgroundEsculetin is a natural coumarin derivative from various plants with multiple pharmacological effects. Hence, the present study was undertaken to explore the cardio protective potential of esculetin against isoproterenol induced myocardial toxicity in rats. Methods The treatment schedule was fixed for 28 days and the rats were divided into five groups of six each. Rats of group I received the normal saline and served as normal control, group II was received ISO (100mg/kg body weight) for last two consecutive days of the study and served as disease control. Groups III and IV received esculetin 10 and 20 mg/kg body weight respectively once a day per oral for 28 days along with ISO for last two consecutive days of the study. Cardiac biomarkers such as CK-MB and LDH, membrane bound Na+ /K+ ATPases activity, myocardial lysosomal enzymes activity and tissue antioxidants status were estimated in the heart tissue samples. The histopathological changes in the myocardium were also assessed. Further, DPPH assay was done to evaluate the free radicals scavenging potential of esculetin. Cytoxicity assay, intracellular ROS levels by DCFDA assay and m-RNA expression of TNF-α, IL-6 and NF-κB by quantitative RT-PCR in H9c2 cell lines.ResultsThe increased levels of CK-MB, LDH, LPO, myocardial lysosomal enzymes and membrane bound Na+ /K+ ATPase levels by ISO administration was significantly increased with concomitant decrease in tissue antioxidant enzymes such as GSH, Catalase, and SOD. Pre-treatment with esculetin for 28 days has significantly decreased the levels of cardiac bio-markers, lysosomal enzymes, membrane bound Na+ /K+ ATPase levels as well as Lipid peroxides which is in contrary to the ISO group. Amelioration of the antioxidant levels were also found in esculetin treated groups. Histopathological examination of heart reveals that myocardial degeneration, mononuclear cell infiltration was noticed in ISO treated rats, whereas the same was restored with esculetin treatment. In H9C2 cell lines esculetin could effectively reduced intracellular ROS inhibition and m-RNA expression of pro-inflammatory cytokines including TNF-α, IL-6 and NF-κB to prevent apoptosis or cell necrosis. Conclusion The study provides the evidence of cardioprotective potentials of esculetin against isoproterenol induced myocardial infarction by antioxidant and myocardial membrane stabilization along with in vitro protection from arsenic induced ROS cell necrosis or apoptosis in H9C2 cells.
Solar Radiation Distribution Method for a Photovoltaic Greenhouse based on the Maximization of Annual Economic Benefits
Authors: Wang Yi , Zhou Tiejun , Zhou Weiji 
Published: March 2021

Abstract
A solar radiation distribution method is proposed based on the maximization of economic benefits for photovoltaic power generation and agricultural production in a photovoltaic greenhouse to solve the problem of low overall economic benefits because of an unreasonable solar radiation distribution between photovoltaic power generation and agricultural production in the photovoltaic greenhouse. First, a mathematical model of the solar radiation yield of photovoltaic greenhouse crops is proposed based on a rectangular hyperbolic modified light response model of crops to represent the relationship between solar radiation energy and crop production. Second, a mathematical model of the average annual revenue of a photovoltaic greenhouse is established to determine the maximum annual economic benefit of the photovoltaic greenhouse, and the model is constrained by the requirements of the light intensity of photovoltaic power generation and environmental conditions for the growth of greenhouse crops. Finally, the correctness of the model is verified by actual operation data of a photovoltaic greenhouse in Xinjiang, and the optimal solar radiation distribution proportion is calculated. This study provides theoretical support for the design of photovoltaic greenhouses.
Ventilatory Responses During and Following Hypercapnic Gas Challenge are Impaired in Male but Not Female Endothelial NOS Knock-out Mice
Authors: Getsy Paulina M. , Sundararajan Sripriya , May Walter J. , vonSchill Graham , McLaughlin Dylan K. , Palmer Lisa A. , Lewis Stephen J. 
Published: March 2021

Abstract
The roles of endothelial nitric oxide synthase (eNOS) in the ventilatory responses during and after a hypercapnic gas challenge (HCC, 5% CO2, 21% O2, 74% N2) were assessed in freely moving female and male wild-type (WT) C57BL6 mice and eNOS knock-out (eNOS-/-) mice of C57BL6 background. HCC elicited an array of ventilatory responses that were similar in male and female WT mice, such as increases in breathing frequency (with falls in inspiratory and expiratory times), and increases in tidal volume, minute ventilation, peak inspiratory and expiratory flows, and inspiratory and expiratory drives. eNOS-/- male mice had smaller increases in minute ventilation, peak inspiratory flow and inspiratory drive, and smaller decreases in inspiratory time than WT males. Ventilatory responses in female eNOS-/- mice were similar to those in female WT mice. The ventilatory excitatory phase upon return to room-air was equal in male and female WT mice. However, the post-HCC increases in frequency of breathing (and decreases in inspiratory times), and increases in tidal volume, minute ventilation, inspiratory and expiratory drives, and peak inspiratory and expiratory flows in male eNOS-/- mice were smaller than in male WT mice. In contrast, the post-HCC responses in female eNOS-/- mice were equal to those of the female WT mice. These findings provide the first evidence that the loss of eNOS affects the ventilatory responses during and after HCC in male C57BL6 mice, whereas female C57BL6 mice can compensate for the loss of eNOS, at least in respect to triggering ventilatory responses to HCC.
Determination of isoprostane 8‐iso‐PGF2α and prostaglandin GF2α in plasma and intestine of specific-pathogen-free chickens challenged with Eimeria maxima
Authors: Petrone-Garcia Victor M. , Lopez-Arellano Raquel , Patiño Gabriela Rodríguez , Rodríguez Miriam A. Castillo , Hernandez-Patlan Daniel , Solis-Cruz Bruno , Hernandez-Velasco Xochitl , Alba-Hurtado Fernando , Vuong Christine N. , Castellanos-Huerta Inkar , Tellez-Isaias Guillermo 
Published: March 2021

Abstract
The purpose of this study was to evaluate and determine the concentration of isoprostane 8‐iso‐PGF2α and prostaglandin GF2α (PGF2α) from plasma and intestine in specific pathogen-free (SPF) chickens challenged with Eimeria maxima (EM) using solid‐phase microextraction and ultra‐performance liquid chromatography/tandem mass spectrometry. Forty one-day-old male SPF chickens were randomly allocated to one of two groups with four replicates (n=5 chickens/replicate). Groups consisted of Control (no challenge) or the Challenge group EM (40,000 sporulated oocysts/bird). At day 7 and 9 post-challenge, half of the chickens were euthanized in both groups to determine plasmatic and enteric concentrations of isoprostane 8‐iso‐PGF2α and PGF2α. Enteric levels of both 8‐iso‐PGF2α and PGF2α were significantly increased at 7 (8‐iso‐PGF2α P=0.0000252; PGF2α P=0.00000268) and 9 days (8‐iso‐PGF2α P=0.000000717; PGF2α P=0.00000222) post-challenge compared to non-challenge control chickens. However, plasma levels of isoprostane 8‐iso‐PGF2α and PGF2α were similar in both groups. A significant reduction (P=0.0000095) in oocyst excretion was observed in chickens at 9 days post-challenge compared to 7 days. Chickens challenged with EM showed an inflammatory response associated with significant increases in enteric PGF2α and 8-Iso-PGF2α, suggesting that the active disease phase was accompanied by inflammation and oxidative stress within the intestinal layer.
Taurine reprograms mammary-gland metabolism and alleviates inflammation induced by Streptococcus uberis
Authors: Lan Riguo , Wan Zhixin , Xu Yuanyuan , Wang Zhenglei , Fu Shaodong , Zhou Yuanyuan , Lin Xinguang , Han Xiangan , Luo Zhenhua , Miao Jinfeng , Yin Yulong 
Published: March 2021

Abstract
Streptococcus uberis (S. uberis) is an important pathogen causing mastitis,which causes continuous inflammation and dysfunction of mammary glands and leads to enormous economic losses. Most research on infection continues to be microbial metabolism-centric, and many overlook the fact that pathogens require energy from host. In this perspective, we uncover metabolic reprogramming during host immune responses is associated with infection-driven inflammation, particularly when caused by intracellular bacteria. Taurine, a metabolic regulator, has been shown to effectively ameliorate metabolic diseases. We evaluated the role of taurine in the metabolic regulation of S. uberis-induced mastitis. Metabolic profiling indicates that S. uberis exposure triggers inflammation and metabolic dysfunction of msmmary glands and mammary epithelial cells (MECs, the main functional cells in mammary glands). Challenge with S. uberis upregulates glycolysis and oxidative phosphorylation in MECs. Pretreatment with taurine restores metabolic homeostasis, reverses metabolic dysfunction by decrease of lipid, amino acid and especially energy disturbance in the infectious context, and alleviates excessive inflammatory responses. These outcomes depend on taurine-mediated activation of the AMPK–mTOR pathway, which inhibits the over activation of inflammatory responses and alleviates cellular damage. Thus, metabolic homeostasis is essential for reducing inflammation. Metabolic modulation can be used as a prophylactic strategy against mastitis. IMPORTANCE: Mastitis, especially caused by Streptococcus uberis, induces continuous inflammation and dysfunction of mammary glands, leading to enormous economic losses in dairy industry. Mammary epithelial cells (MECs) are mammary gland-resident major functional cells which occupy a significant role in maintaining homeostasis in mammary glands. MECs can adopt an inflammatory trending in response to S. uberis infection and try to clear these intracellular bacteria. Researchers at Nanjing Agricultural University in China found that S. uberis induces MECs acute inflammation caused by metabolic dysregulation, that is, increased oxidative phosphorylation (OXPHOS) and glycolysis. However, taurine pretreatment can enhance metabolic fitness and anti-inflammatory activity. As a potential therapy, metabolic regulation with taurine restored MECs homeostasis and reduced excessive inflammation. The findings suggest that modulating MECS metabolism might be a new therapeutic strategy for mastitis as well as other bacterial infections.