bluuebunny/crossref_metadata_2025_split huggingface dataset:
Taxonomy, Phylogeny, Molecular Dating and Ancestral State Reconstruction of Xylariomycetidae (Sordariomycetes)
Authors: Samarakoon Milan C. , Hyde Kevin D , Maharachchikumbura Sajeewa S. N. , Stadler Marc , Jones E. B. Gareth , Promputtha Itthayakorn , Suwannarach Nakarin , Camporesi Erio , Bulgakov Timur S. , Liu Jian-Kui 
Published: October 2021

Abstract
Xylariomycetidae ( Ascomycota ) is a highly diversified group with variable stromatic characters. Our research focused on inconspicuous stromatic xylarialean taxa from China, Italy, Russia, Thailand and the United Kingdom. Detailed morphological descriptions, illustrations and combined ITS-LSU- rpb 2- tub 2- tef 1 phylogenies revealed 38 taxa from our collections belonging to Amphisphaeriales and Xylariales . A new family ( Appendicosporaceae ), five new genera ( Magnostiolata , Melanostictus , Neoamphisphaeria , Nigropunctata and Paravamsapriya ), 27 new species ( Acrocordiella photiniicola , Allocryptovalsa sichuanensis , Amphisphaeria parvispora , Anthostomella lamiacearum , Apiospora guiyangensis , Ap. sichuanensis , Biscogniauxia magna , Eutypa camelliae , Helicogermslita clypeata , Hypocopra zeae , Magnostiolata mucida , Melanostictus longiostiolatus , Me. thailandicus , Nemania longipedicellata , Ne. delonicis , Ne. paraphysata , Ne. thailandensis , Neoamphisphaeria hyalinospora , Neoanthostomella bambusicola , Nigropunctata bambusicola , Ni. nigrocircularis , Ni. thailandica , Occultitheca rosae , Paravamsapriya ostiolata , Peroneutypa leucaenae , Seiridium italicum and Vamsapriya mucosa ) and seven new host/geographical records are introduced and reported. Divergence time estimates indicate that Delonicicolales diverged from Amphisphaeriales + Xylariales at 161 (123–197) MYA. Amphisphaeriales and Xylariales diverged 154 (117–190) MYA with a crown age of 127 (92–165) MYA and 147 (111–184) MYA, respectively. Appendicosporaceae ( Amphisphaeriales ) has a stem age of 89 (65–117) MYA. Ancestral character state reconstruction indicates that astromatic, clypeate ascomata with aseptate, hyaline ascospores that lack germ slits may probably be ancestral Xylariomycetidae having plant-fungal endophytic associations. The Amphisphaeriales remained mostly astromatic with common septate, hyaline ascospores. Stromatic variations may have developed mostly during the Cretaceous period. Brown ascospores are common in Xylariales , but they first appeared in Amphisphaeriaceae , Melogrammataceae and Sporocadaceae during the early Cretaceous. The ascospore germ slits appeared only in Xylariales during the Cretaceous after the divergence of Lopadostomataceae . Hyaline, filiform and apiospores may have appeared as separate lineages providing the basis to Xylariaceae , which may have diverged independently. The future classification of polyphyletic xylarialean taxa will not be based on stromatic variations, but the type of ring, the colour of the ascospores, and the presence or absence of the type of germ slit.
WITHDRAWN: Laboratory Study On Mechanical Response And Failure Mechanism of Red-Bed Soft Rock Under Water-Rock Interaction
Authors: Liu Chi , Liu Xiaoli , Sun Huan , Wang Mingyang , Wu Chunlu , Wang Enzhi , Wang Sijing 
Published: October 2021

Abstract

Red-bed soft rock is a geomaterial that displays special deformation and failure characteristics. The stability of red-bed slopes can be negatively impacted by water and stepped excavation disturbance; however, there is limited research regarding the mechanical behavior and failure characteristics of red-bed soft rock under the action of water-rock hydro-mechanical coupling. In this study, to explore the mechanical response and failure mechanisms of red-bed soft rock under coupled water-rock hydro-mechanical action, a visual experimental platform based on digital radiography and a multi-level loading device was constructed. Angiography was used to visualize the rock fracture process by replacing fissure water with a contrast medium. Multi-level loading was applied to cubic red-bed mudstone samples, and acoustic emission signals, stress, flow rate, and digital radiography images were collected during the failure process. An original image processing method based on Hough transform and a convolutional neural network was used to segment and extract cracks from the imagery, and fissure water flow characteristics, rock mechanical response, and crack evolution were analyzed in detail (Liu et al., 2015; Lv et al., 2013, 2014). Results showed that when the Felicity ratio FR was lower than 1.2, water could induce secondary "water-damaged cracks" in the red-bed samples. Study findings were used to highlight the importance of improved early-warning methods for rainfall-induced landslides at an engineering scale. The original experimental platform proposed and evaluated in this study provides a new and powerful tool to investigate the mechanical behavior of different rock types under the action of water-rock hydro-mechanical coupling at a laboratory scale. These findings will facilitate improved disaster prevention strategies for red-bed geological bodies.
Modest weight loss improves leptin to adiponectin ratio and induces insulin and leptin resensitivization in individuals with obesity.
Authors: Isaksen Victoria Therese , Larsen Maria Arlen , Goll Rasmus , Paulssen Eyvind J. , Florholmen Jon Ragnar 
Published: October 2021

Abstract
Background

Weight loss is important to reduce the risk of metabolic complications in obese individuals, in whom dysregulated adipokines play a central role. This study aims to investigate whether dysregulated adipokines and postprandial triglycerides (TG) improve with a modest weight loss.

Methods

Individuals with obesity were recruited among patients at the University Hospital of North Norway and the Stamina Health weight loss rehabilitation program. We measured resting energy expenditure (REE), and calculated the Homeostasis Model Assessment of Insulin Resistance (HOMA-IR), leptin to adiponectin (L:A) ratio, indirect leptin sensitivity (REE:leptin ratio), postprandial TG clearance at 6 h, and TG response before and after weight loss. The goal of the weight loss intervention was a loss of ≥5% of initial total body weight.

Results

Of the 28 participants who attended two scheduled assessments, 13 lost ≥5% body weight. HOMA-IR (-23.1%), REE:leptin ratio (+80.1%) and L:A ratio (-45.7%) significantly improved with weight loss, whereas there was no improvement of postprandial TG response or clearance. No significant changes were observed in the non-weight loss group.

Conclusion

Metabolic dysregulation, as insulin and leptin resistance, but not postprandial TG improve with a modest weight loss in individuals with obesity.
Initial assessment of a cancer genomic profile test for patients with metastatic breast cancer: a retrospective study
Authors: Fukada Ippei , Mori Seiichi , Hayashi Naomi , Hosonaga Mari , Yamazaki Masumi , Wang Xiaofei , Kawai Saori , Inagaki Lina , Ozaki Yukinori , Kobayashi Kokoro , Hara Fumikata , Kobayashi Takayuki , Ueki Arisa , Osako Tomo , Tonooka Akiko , Takeuchi Kengo , Ueno Takayuki , Takano Toshimi , Ohno Shinji , Takahashi Shunji 
Published: October 2021

Abstract
Comprehensive cancer genomic profile (CGP) tests are being implemented under Japanese universal health insurance system. However, the clinical usefulness of CGP test for breast cancer patients has not been evaluated. Of the 310 patients who underwent CGP testing at our institution between November 2019 and April 2021, 35 patients with metastatic breast cancer whose treatment strategy was discussed by our molecular tumor board within the study period were investigated after exclusion of 2 cases that could not be analyzed. The turn-around time, drug accessibility, and germline identification detection were evaluated. The subtype was luminal in 20 patients (57.1%), triple-negative in 12 patients (34.3%), and luminal-HER2 in 3 patients (8.6%). Actionable gene mutations were detected in 30 patients (85.7%), and 7 patients (20.0%) were recommended for clinical trial participation, with the drug administered to 2 patients (5.7%). Three patients (8.6%) died due to disease progression before the test results were disclosed. We report the results of an initial assessment of the utility of CGP testing for patients with metastatic breast cancer under Japanese universal health insurance system. Conducting CGP tests at a more appropriate time could provide patients with greater benefit from treatments based on their specific gene mutations.
CO2 supplementation eliminates sugar-rich media requirement for plant propagation using a simple inexpensive temporary immersion photobioreactor
Authors: Trauger Marena , Hile April , Sreeni Krishnan , Shouse Eva Mei , Bhatt Jishnu , Lai Tina , Mohandass Ramya , Tripathi Leena , Ogden Aaron J. , Curtis Wayne R. 
Published: October 2021

Abstract
In-vitro plant propagation systems such as Temporary Immersion Bioreactors (TIBs) are valuable tools that enable production of disease-free plants with improved traits. However, TIB systems can be expensive, difficult to implement, and prone to contamination due to sugar rich propagation media. Using rapidly growing chicory root cultures to expedite design-build-test cycles, we report here an improved, low-cost version of a previously reported Hydrostatically-driven TIB (Hy-TIB) that facilitates economical use of gas mixtures. Bioreactor improvements include decreased material costs, expanded modes of operation, and a horizontal orientation of a plastic film plant growth chambers that increase propagule light exposure. To take advantage of these improvements, we describe here experiments that evaluate the impacts of elevated CO2 on propagation of cacao (Theobroma cacao) secondary embryos and nodal cultures of yam (Dioscorea spp.) during both phototrophic and photomixotrophic growth. Our experiments show that elevated CO2 during plant propagation significantly improved both cacao and yam propagule development and eliminated the need for supplemental sugars in tissue culture growth media. Thus, our improved Hy-TIB shows potential as a simple, low-cost, and scalable propagation platform with cost-effective gas composition control and reduced risk of contamination overgrowth. We provide detailed instructions for assembly of this Hy-TIB design and discuss the implications of its adoption in food-insecure regions of the world.
Dietary strategies of Pleistocene Pongo sp. and Homo erectus on Java (Indonesia)
Authors: Kubat Jülide , Nava Alessia , Bondioli Luca , Dean Christopher , Zanolli Clément , Bourgon Nicolas , Bacon Anne-Marie , Demeter Fabrice , Peripoli Beatrice , Mahoney Patrick , Albert Richard , Kullmer Ottmar , Schrenk Friedemann , Müller Wolfgang 
Published: October 2021

Abstract
During the early Pleistocene, Java was inhabited by a high variety of hominid and hominin taxa with hitherto unclear seasonal dietary demands. We undertook the first geochemical analyses of Pongo sp., Homo erectus and mammalian Pleistocene teeth from Sangiran. We reconstructed past dietary strategies at daily resolution and inferred sub-seasonal ecological patterns. Histologically-controlled spatially-resolved elemental analyses by LA-ICPMS, confirmed the preservation of authentic biogenic signals despite very weak diagenetic overprint. The Sr/Ca record of mammals is in line with expected trophic positions, contextualizing fossil hominid diet. Herbivorous Pongo sp. displays marked seasonal cycles with ~3-month-long strongly elevated Sr/Ca peaks, reflecting highly nutritional plant food during monsoon seasons. Lower Sr/Ca signals suggest different food availability during the dry season. In contrast, omnivorous Homo erectus shows low and less accentuated intra-annual Sr/Ca variability. We infer that Homo erectus maintained its nutritional demands independent of seasonal fluctuations by exploiting the regional diversity of high-quality food resources.
Transmembrane Protein 108 Inhibits the Proliferation and Myelination of Oligodendrocyte Lineage Cells in the Corpus Callosum
Authors: Wu Yongqiang , Zhong Yanzi , Liao Xufeng , Miao Xiangguang , Yu Jianbo , Lai Xinsheng , Zhang Yu , Ma Chaolin , Wang Shunqi 
Published: October 2021

Abstract
Background: Abnormal white matter is a common neurobiological change in bipolar disorder, and dysregulation of myelination in oligodendrocytes is the cause. Transmembrane protein 108 (Tmem108), as a susceptible gene of bipolar disorder, is expressed higher in oligodendrocyte lineage cells than any other lineage cells in the central nervous system. Moreover, Tmem108 mutant mice exhibit mania-like behaviors, belonging to one of the signs of bipolar disorder. However, it is unknown whether Tmem108 regulates myelination of the oligodendrocytes.Results: Tmem108 expression in the corpus callosum decreased with the development, and hypermyelination of the corpus callosum was found in Tmem108 mutant mice, accompanying high expression of myelin basic protein. Strikingly, both oligodendrocyte progenitor cell proliferation and oligodendrocyte myelination were enhanced in the mutant mice. Furthermore, the mutant mice exhibited mania-like behavior after acute restraint stress and were susceptible to drug-induced epilepsy. Conclusions: Tmem108 inhibited oligodendrocyte progenitor cell proliferation and mitigated oligodendrocyte maturation in the corpus callosum, which may also provide a new role of Tmem108 involving bipolar disorder pathogenesis.
Continued Adherence to Community-Based Health Insurance Scheme in Two Districts of Northeast Ethiopia: Application of Accelerated Failure Time Shared Frailty Models
Authors: Hussien Mohammed , Azage Muluken , Bayou Negalign Berhanu 
Published: October 2021

Abstract
Background: The sustainability of a voluntary community-based health insurance scheme depends to a greater extent on its ability to retain members. In low- and middle-income countries, high rate of member dropout has been a great concern for such schemes. Although few studies had investigated the factors influencing dropout decisions, none of these looked into how long and why members adhere to the scheme. The purpose of this study was to determine the factors affecting time to drop out while accounting for the influence of cluster-level variables. Methods: A community-based cross-sectional study was conducted among 1232 rural households who have ever been enrolled in two community-based health insurance schemes. A household survey was conducted using a mobile data collection platform. The Kaplan-Meier estimates were used to compare the time to drop out among subgroups. To identify predictors of time to drop out, a multivariable analysis was done using the accelerated failure time shared frailty models. The degree of association was assessed using the acceleration factor (δ) and statistical significance was determined at 95% confidence interval. Results: Results of the multivariable analysis revealed that marital status of the respondents (δ=1.614; 95% CI: 1.221–2.134), household size (δ=1.167; 95% CI: 1.012–1.344), presence of chronic illness (δ=1.421; 95% CI: 1.163–1.736), hospitalization history (δ=1.308; 95% CI: 1.120–1.529), higher perceived quality of care (δ=1.323; 95% CI: 1.101–1.589), perceived risk protection (δ=1.220; 95% CI: 1.029–1.446), and higher trust in the scheme (δ=1.729; 95% CI: 1.428–2.095) were significant predictors of time to drop out at p-value < 0.05. Conclusions: The study identified evidence suggestive of adverse selection in the schemes. The fact that larger households remain in the scheme indicates the need to reconsider the premium level in line with household size to attract small size households. Issues that are under the control of the scheme and the healthcare system can be adjusted to increase membership adherence. Resolving problems related to the quality of health care can be a cross-cutting area of ​​intervention to retain members by building trust in the scheme and enhancing the risk protection ability of the schemes.
Inhibiting Effects of Down-regulating of Fascin 1 on Proliferation and Migration in Hepatoma Cells
Authors: Gu Wanglu , Wang Guilan , Zhang Xinyang , Chen Li , Zhou Jiaming 
Published: October 2021

Abstract
Objective: To investigate the inhibiting effects of fascin 1 gene knock-down on the proliferation and migration of hepatoma cells by means of small interfering RNA (siRNA).Methods: SiRNA targeting fascin 1 gene (si-fascin) and non-specific sequence siRNA (si-NC）were constructed and transfected into human hepatoma cell lines (HepG2 and Huh7) to down-regulate the expression of fascin 1. RT-qPCR, Western blotting, and Immunofluorescence technique were used to evaluate the efficiency of si-fascin. The proliferation and migration of cells were detected by MTT method and Transwell experiments, and the protein expression of genes related to proliferation and migration in cells were detected by Western blotting. The apoptosis and pseudopodia formation of cells were observed under scanning electron microscope (SEM).Results: Compared with human normal liver cells (LO2), the expressions of fascin 1 mRNA and protein were significantly higher in HepG2 and Huh7 cells. The expression of fascin 1 was overall inhibited in HepG2 and Huh7 cells transfected by the constructed four si-fascins, among which, fascin_siR3 had the highest inhibitory efficiency, therefore was selected in this study. In HepG2 and Huh7 cells transfected by si-fascin significant knock-down target gene expression, while reducing cell proliferation, migration and the formation of pseudopods, and causes reduced protein expression associated with proliferation and migration. Conclusion: This study further confirmed that fascin 1 gene has the function of promoting hepatoma cells proliferation and migration, suggesting that downregulating the expression of fascin 1 in hepatoma cells may be one of the strategies to intervene in liver cancer.
Nanostructured Ge and GeSn Films by High-pressure He Plasma Sputtering for High-capacity Li ion Battery Anodes
Authors: Uchida Giichiro , Nagai Kenta , Habu Yuma , Hayashi Junki , Ikebe Yumiko , Hiramatsu Mineo , Narishige Ryota , Itagaki Naho , Shiratani Masaharu , Setsuhara Yuichi 
Published: October 2021

Abstract
We fabricated nanostructured Ge and GeSn films in He radio-frequency magnetron plasma sputtering deposition. Monodisperse amorphous Ge and GeSn nanoparticles of 30-40 nm in size were orderly arranged without aggregation by off-axis sputtering deposition in high He-gas-pressure range of 0.1 Torr. The Ge film porosity was over 30%. We tested the charge/discharge cycle performance of Li-ion batteries with nanostructured Ge and GeSn anodes. The Ge anode with an ordered arrangement of nanoparticles showed Li-storage capacity of 565 mAh/g after the 60th. The capacity retention was markedly improved by the addition of 3at% Sn in Ge anode. GeSn anode (3at% Sn) achieved a higher capacity of 1,128 mAh/g after 60th cycles with 92% capacity retention. Precise control of the nano-morphology and electrical characteristics by a single step procedure using low temperature plasma is effective for stable cycling of high-capacity Ge anodes.
Meta-QTLs, ortho-MQTLs and candidate genes for thermotolerance in wheat (Triticum aestivum L.)
Authors: Kumar Sourabh , Singh Vivudh Pratap , Saini Dinesh Kumar , Sharma Hemant , Saripalli Gautam , Kumar Sachin , Balyan Harindra Singh , Gupta Pushpendra Kumar 
Published: October 2021

Abstract
Meta-QTL analysis for thermotolerance in wheat was conducted to identify robust meta-QTLs (MQTLs). In this study, 441 QTLs related to 31 heat-responsive traits were projected on the consensus map saturated with 50,310 markers. This exercise resulted in the identification of 85 MQTLs with confidence interval (CI) ranging from 0.11 to 34.9 cM with an average of 5.6 cM. This amounted to a 2.96 fold reduction relative to the mean CI (16.5 cM) of the QTLs used. Seventy-seven (77) of these MQTLs were also verified with the results of recent genome-wide association studies (GWAS). These MQTLs included seven MQTLs that are particularly useful for breeding purposes (we called them Breeders’ MQTLs). Seven ortho-MQTLs between wheat and rice genomes were also identified using synteny and collinearity. The MQTLs were used for the identification of 1,704 candidate genes (CGs). In silico expression analysis of these CGs permitted identification of 182 differentially expressed genes (DEGs), which included 36 high-confidence candidate genes (CGs) with known functions previously reported to be important for thermotolerance. These high confidence CGs encoded proteins belonging to the following families: protein kinase, WD40 repeat, glycosyltransferase, ribosomal protein, SNARE associated Golgi protein, GDSL lipase/esterase, SANT/Myb domain, K homology domain, etc. Thus, the present study resulted in the identification of MQTLs (including breeders’ MQTLs), ortho-MQTLs, and underlying CGs, which could prove useful not only for molecular breeding for the development of thermotolerant wheat cultivars but also for future studies focused on understanding the molecular basis of thermotolerance.
Awareness and Health Risk Protection Behaviours of Scavengers in the Gbalahi Landfill Site in the Era of Sustainable Development, Ghana
Authors: Nuripuoh Joseph Gyea , Duwiejuah Abudu Ballu , Bakobie Noel 
Published: October 2021

Abstract
Scavenging is a pivotal in achieving sustainable waste management, environment health and economic development. The purpose of the study was to assess the practices, knowledge, perception and health risk protection behaviours of waste scavengers in the Gbalahi landfill site. A total of 60 scavengers were conveniently sampled and interviewed. The study showed 83% of the respondents had an average monthly income between GH¢ 1.00 to GH¢ 100.00 whereas 17% had between GH¢ 101.00 to GH¢ 300.00. The study also revealed 93% of the respondents sort waste using hooks and their bare hands. The respondents that have ever been physically abused by other scavengers were 62%. A significant number of scavengers believed they have been fortified against “dirt diseases” during their childhood and have developed natural immunity against diseases. The knowledge of scavengers was skewed towards economic benefits as they viewed scavenging as a survival strategy. Safety and protection practices are limited to the use of pieces of clothes to cover the nose, wearing of multiple clothes and worn-out boots recovered from the landfill. Discrimination and physical abuse posed a seemingly significant psychological health risk to majority of them. Covid-19 health risks behaviours, majority of the respondents risk being exposed to the virus and pathogens. Scavengers should be provided with personal protective equipment and / or strictly made to obey safety and protection protocols.