bluuebunny/crossref_metadata_2025_split huggingface dataset:
5. THE ADDITION OF SEHAT, AN ADAPTATION OF DASH DIET, IN ONGOING REGIMENT OF CAPTOPRIL IN A HYPERTENSION PATIENT AT PRIMARY HEALTHCARE SETTING: AN EXPERIMENTAL STUDY
Authors: Setiabudi PA , Ramadhani FR , Kautsarani I , Syabani HS 
Published: May 2021

Background:
Hypertension still becomes a menacing worldwide problem. Ninety percent of people over 55 years will develop hypertension. DASH (Dietary Approaches to Stop Hypertension) diet proven to have substantial blood pressure (BP)-lowering action. Captopril is one of the first-line hypertension treatments in Indonesia since it was accessible and affordable. We conducted SEHAT (Seleksi Gaya Hidup Anti Hipertensi), a more adapted hypertension intervention program based on the DASH diet.

Objective:
Here we are going to report the results of adding SEHAT in an ongoing regiment of captopril in a hypertension patient in a primary healthcare setting.

Method:
: We conducted SEHAT counseling in the monthly “PROLANIS” in Sutojayan Primary Health Care. 14 patients with essential hypertension divided into 2 groups: 7 patients accustomed to SEHAT and received 3 × 25 mg Captopril, while 7 others with only captopril consumption as a control group. We measured the difference value of systolic, diastolic, and Mean Arterial Pressure (MAP) pre and post 4-weeks intervention. Statistical analysis was done using an independent t-test.

Result:
Based on mmHg measurement, there were a greater reduction of systolic blood pressure (−11.43 vs 1.71; p = 0.259), diastolic blood pressure (−4.29 vs −2.57; p = 0.833), and MAP (−1.14 vs −6.86; p = 0.517) between SEHAT and control group respectively.

Conclusion:
SEHAT has the potency to become a promising antihypertension program in primary health care. As there was no significant result, further studies need to be carried out with a greater sample to strengthen its efficacy.
9. HYPERTENSION WITH COVID 19 INFECTION AT THE HOSPITAL OF THE CHRISTIAN UNIVERSITY OF INDONESIA, JAKARTA PERIOD MAY TO OCTOBER 2020
Authors: Suling Frits Reinier Wantian , Raspati Zega A , Rantung Nolly Octavianes Habel , Chandramin  , Puspitasari Febtusia 
Published: May 2021

Background:
This study discussed patients with covid infection 19 who were admitted to Indonesian Christian university hospital with comorbid hypertension. On December 31, 2019, China reported a case of mysterious pneumonia with no known cause. Within 3 days, the number of patients with these cases was 44 patients and continues to increase until now there are thousands of cases. Initially epidemiological data showed 66% of patients were related or exposed to a seafood market or live market in Wuhan, Hubei Province, China. (2019-nCoV) .2 On February 11, 2020, the World Health Organization named the new virus Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) and the name of the disease as Coronavirus Disease 2019 (COVID-19).
Hypertension is one of the most common comorbids in Covid-19 patients. Hypertension is also widely available in Covid-19 patients who experience ARDS. It is currently unclear whether uncontrolled hypertension is a risk factor for contracting Covid-19, but controlling blood pressure is still considered important to reduce the burden of disease. SARS-CoV-2, the virus that causes Covid-19, binds to ACE2 in the lungs to enter cells, resulting in the use of angiotensin converting enzymes (ACE inhibitors) and angiotensin receptor blockers (ARBs), 2 classes of drugs that are frequently used in controlling hypertension, questionable benefit or harm.

Objective:
Studying patients with covid 19 and their accompanying comorbids who were treated at the Hospital of the Faculty of Medicine of the Indonesian Christian University, Jakarta.

Method:
This type of research is observational research. This study only observed patients. Study design of all treated patients with COVID-19. Research Location: General Hospital of the Christian University of Indonesia, Research Time: May to October 2020.

Result:
It showed 50 people entered the inclusion criteria, hypertension with the most covid 19, 26 people (52%), type 2 diabetes mellitus with covid 19, 22 people (44%), CKD on hemodialysis with covid 19, 4 people (8%), CKD Non HD with covid 19, 3 people (6%). There were 2 patients with heart failure with covid 19 (4%), 2 people with hyperthyroidism with covid 19 (4%). Meanwhile, the ages of 61–70 years (40%) have a lot of Covid 19 in the period that date.

Conclusion:
Covid 19 patients with comorbid hypertension and type 2 diabetes mellitus were mostly found with ages between 61-70 years.
15. BODY MASS INDEX AND HYPERTENSION IN INDONESIAN HAJJ PILGRIMS
Authors: Utami Eka , Ardiana Meity 
Published: May 2021

Background:
Indonesia has the largest number of yearly hajj pilgrim around the world. Most of those pilgrims are elderly obese with comorbidity such as hypertension.

Objective:
The aim of this study is to determine correlation between body mass index and hypertension among East Java's pilgrims in 2017 to 2019.

Method:
This study is observational analytic as in cross sectional analysis from East Java pilgrims data between 2017 to 2019. Data was collected by trained hajj health worker and then analyzed by spearman rank correlation test. Body mass index was classified by World Health Organization (WHO) while hypertension by Joint National Committee (JNC) 8.

Result:
There was a total of 105.988 data was collected in which 392 data are invalid due to inappropriate value. Almost half (45.47%) of the pilgrims were normal body mass index yet only 4871 (4.6%) of the pilgrims were underweight. There were 38.403 (36.36%) pilgrims categorized as overweight while 14.301 (13.54%) were obese. However, only 54.120 (1.7%) had normal systolic blood pressure. Further, alarmingly, 31.096 (29.44%) were in pre hypertension stage. There were only 13.757 (13.02%) and 6623 (6.2%) pilgrims categorized having grade one and two hypertension respectively. From the spearmen test analysis result, there was a significant correlation between body mass index and hypertension (p = 0.000, r = 1).

Conclusion:
In sum, there was a strong linear significant relationship between body mass index and systolic blood pressure.
42. INTRADIALYTIC HYPERTENSION AND SIX MONTH – ALL CAUSE MORTALITY IN HEMODIALYSIS PATIENTS
Authors: Perdhana Langgeng , Chasani Shofa 
Published: May 2021

Background:
Hemodialysis is safe and beneficial for CRF patient. However, there are several complications during hemodialysis process including intradialytic hypertension (IDH). IDH has been associated with poor outcomes in hemodialysis patients. But only a few studies have investigated the relationship of IDH with mortality in patients undergoing hemodialysis.

Objective:
This study aims to determine the relationship between IDH and 6 month-all cause mortality in hemodialysis patients.

Method:
The cohort design study was carried out in October 2019 to March 2020 at the Hemodialysis Unit of Roemani Muhammadiyah Hospital Semarang. The dependent variable was IDH that defined as rise in Mean Arterial Pressure (MAP) >15 mmHg within or immediately post dialysis. The mortality was investigated over 6 months after the baseline measurements.

Result:
Among 85 respondents, it was found that 22 (25.9%) respondents categorized into IDH group and 63 (74.1%) categorized into non IDH group. The respondents consisted of 54 (63.5%) males and 31 (36.5%) females. The mean delta MAP in the IDH and non IDH group was (mean ± standard error, 22.6 ± 7.9 vs −5 ± 12.3). From the Kaplan Meier survival curve described that non IDH group had higher survival rate than the IDH group (96.8% vs 77.3%, p = 0.004). From Cox regression study we found a significant relationship between IDH and 6-month all cause mortality in hemodialysis patients (p = 0.018; Hazard ratio: 7.305).

Conclusion:
There was a significant relationship between IDH and 6 month – all cause mortality in hemodialysis patients.
45. BLOOD PRESSURE ON ADMISSION IN PATIENTS WITH ACUTE CORONARY SYNDROME COMPLICATED BY ACUTE RESPIRATORY FAILURE
Authors: Sain Azlan , Widyantoro Bambang , Harlyjoy Beta Canina , Juzar Dafsah Arifa 
Published: May 2021

Background:
Patient with Acute Coronary Syndrome (ACS) can present with varying degrees of hemodynamic compromise, fluid overload and end-organ hypoperfusion. In addition, these patients typically have high filling pressures, biventricular failure and secondary pulmonary hypertension resulting in decreased gas exchange and increased work of breathing, contributing to acute respiratory failure (ARF). Blood pressure is an easily obtainable diagnostic tool. Ample studies had shown the role of BP on admission as a prognostic instrument in ACS patient.

Objective:
To observe the relationship of BP on admission and ARF as ACS complication at National Cardiovascular Centre Harapan Kita (NCCHK)

Methods:
We collected medical record data of acute coronary syndrome patients in NCCHK from January to December 2020.

Results:
1.383 samples were obtained. ARF occurs in 4.2% of our patients. Systolic Blood Pressure (SBP) in patient with respiratory failure was higher (130: 60–255 mmHg) compared with patient without respiratory failure (116: 61–255 mmHg, p = 0.001). Diastolic Blood Pressure (DBP) was also higher in patient with ARF (77: 30–218 vs 70: 38–130 mmHg, p = 0.001). Further analysis with chi-square test showed that patient with hypertension on admission was associated with ARF (p = 0.035) with odds ratio 1.8, 95% CI of 1.036 to 3.383 (p = 0.038).

Conclusion:
ACS patients complicated by ARF have higher blood pressure (SBP and DBP) on admission than in ACS patients without respiratory failure
47. PULSE PRESSURE ON ADMISSION AND SHORT-TERM OUTCOME IN ST-SEGMENTS ELEVATION MYOCARDIAL INFARCTION PATIENTS UNDERGOING PRIMARY PERCUTANEOUS INTERVENTION
Authors: Suryawan Willy , Danny Siska Suridanda , Harlyjoy Beta Canina , Sain Azlan , Widyantoro Bambang 
Published: May 2021

Background:
In patients with ST-segment elevation myocardial infarction (STEMI), blood pressure (BP) variables are easily obtainable prognostic tools. Recently, pulse pressure (PP) has been identified as a more powerful prognostic predictor of cardiovascular events compared to systolic blood pressure (SBP) or diastolic blood pressure (DBP) in selected populations.

Objective:
To observe the relationship of BP variables on admission and short-term outcomes in STEMI patients undergoing PCI at National Cardiovascular Center Harapan Kita (NCCHK).

Methods:
We collected medical record data of STEMI patients undergoing primary PCI in NCCHK from January to December 2020.

Results:
Three hundred and forty-four samples were obtained. No in-hospital mortality was found in our population. The median length-of-stay was 5: 3–47 days. In-hospital cardiac arrest rate was 2.9% (10 patients), with rate of rehospitalization of 11.3% (39 patients). In patients experiencing in-hospital cardiac arrest, the SBP was lower (115.40 ± 22.27 vs 126.94 ± 24.48, p = 0.169), DBP was higher (81.50: 56–92 vs 76: 28–128, p = 0.746), and lower PP (34.50: 20–63 vs 48: 6–119, p = 0.020). Further analysis with chi-square test showed that PP < 40 mmHg is associated with in-hospital cardiac arrest (p = 0.023).

Conclusion:
Pulse pressure on admission is associated with in-hospital cardiac arrest in STEMI patients undergoing PCI.
Seatbelts Save Lives, and Spines, in Motor Vehicle Accidents
Authors: Sarwahi Vishal , Atlas Aaron M. , Galina Jesse , Satin Alexander , Dowling Thomas J. , Hasan Sayyida , Amaral Terry D. , Lo Yungtai , Christopherson Nathan , Prince Jose 
Published: May 2021

Study Design.
Database study.

Objective.
The purpose of this study was to use a large, nationwide database to determine prevalence of pediatric spine fractures in the United States, associated injuries, mechanisms of injury (MOI), use of safety devices, and mortality rates.

Summary of Background Data.
Spinal fractures account for 1% to 2% of pediatric injuries. However, they are associated with significant comorbidities and complications. Motor vehicle accidents (MVAs) are most responsible for increased incidence observed.

Methods.
Retrospective review of National Trauma Data Bank between 2009 and 2014 (analysis in 2019) for all vertebral fractures in patients under 18 years of age. Subanalysis included those in MVAs where protective device use data were available. Patient demographics, MOI, geographical and anatomical region of injury, concomitant musculoskeletal/organ injury, protective device usage, hospital length of stay, surgical procedures, and mortality were all analyzed.

Results.
A total of 34,563 patients with 45,430 fractured vertebrae included. Median age was 15 years. Most fractures (63.1%) occurred in patients aged 15 to 17 years, most frequent MOI was MVA (66.8%), and most common geographic location was the South (38%). Males sustained more spine fractures than females, overall (58.4% vs. 41.6%; P < 0.001) and in MVAs (54.4% vs. 45.6%; P < 0.001). Those in MVAs wearing seatbelts had lower odds of cranial (29.6% vs. 70.4%; odds ratio [OR] = 0.85, 95% confidence interval [CI]: 0.82–0.89; P < 0.001) and thoracic (30.1% vs. 69.9%; OR = 0.88, 95% CI: 0.84–0.91; P < 0.001) organ injury, multivertebral (30% vs. 70%; OR = 0.78, 95% CI: 0.73–0.83; P < 0.001) and concomitant nonvertebral fractures (30.9% vs. 69.1%; OR = 0.89, 95% CI:0.73–0.83; P < 0.001), and 21% lower odds of mortality (29.3% vs. 70.7%; OR = 0.79, 95% CI: 0.66–0.94; P = 0.009). Over 70% of drivers were not restrained during MVA, with majority of seatbelt violations incurred by males, ages 15 to 17, in the South.

Conclusion.
Over 60% of pediatric spinal fractures occur in children aged 15 to 17 years, coinciding with the beginning of legal driving. MVA is the most common cause and has significant association with morbidity/mortality. Nearly two- thirds pediatric spinal fractures sustained in MVAs occurred without seatbelts. Absence of seatbelts associated with >20% greater odds of mortality. Ensuring new drivers wear protective devices can greatly reduce morbidity/ mortality associated with MVA.

Level of Evidence: 3
The Chimeric Scapulodorsal Vascularized Latissimus Dorsi Nerve Flap for Immediate Reconstruction of Total Parotidectomy Defects With Facial Nerve Sacrifice
Authors: Li Sean S. , Mangialardi Maria L. , Nguyen Quyen T. , Orosco Ryan K. , Honart Jean F. , Qassemyar Quentin , Kolb Frederic J. 
Published: May 2021

Background
Total parotidectomy with facial nerve sacrifice creates 2 challenging reconstructive problems: restoration of facial contour and facial nerve rehabilitation. Strong evidence suggesting that vascularized nerve grafts are superior to nonvascularized nerve grafts motivated our team to develop a chimeric scapulodorsal flap combining the usual harvestable local tissues with the vascularized latissimus dorsi motor nerve (SD-LDVxN). We present our experiences developing a new program at University of California, San Diego, highlighting our first case here, and present preliminary retrospective results focusing on the functional outcomes of facial nerve reanimation.

Materials and Methods
The first case performed in the United States was a 57-year-old woman with stage IVA left parotid adenoid cystic carcinoma and House-Brackmann grade 6 facial palsy. She underwent total parotidectomy with facial nerve sacrifice and a free chimeric SD-LDVxN flap reconstruction. She had an unremarkable postoperative course, and 3- and 6-month follow-up functional results are reported. Preliminary functional results from our total series of 25 patients were reported.

Results
At her 3-month follow-up, she was a House-Brackmann 5 with a static eFACE score of 37, dynamic eFACE score of 31, and smile eFACE score of 48. At her 6-month follow-up, she was a House-Brackmann 5 with a static eFACE score of 50, dynamic eFACE score of 27, and smile eFACE score of 53. Preliminary results from our total series of 25 patients with an average of 5 years of follow-up were a House-Brackmann 2.5 and eFACE scores of 83.1 for static facial symmetry, 67.5 for dynamic facial symmetry, and 77.7 for smile score. Twenty of the 25 patients had postoperative radiotherapy. No local tumor recurrence had been reported. The average reinnervation time was 9 months and ranged from 3 to 15 months.

Conclusions
The SD-LDVxN flap is a highly resourceful solution to reconstruct complex parotid defects, especially those that sacrifice the facial nerve. The vascularized nerve graft allows for primary facial reanimation. Nerve recovery may be superior to what could be expected with a conventional nerve graft.
Improved Genome Packaging Efficiency of AAV Vectors Using Rep Hybrids
Authors: Mietzsch Mario , Eddington Courtnee , Jose Ariana , Hsi Jane , Chipman Paul , Henley Tom , Choudhry Modassir , McKenna Robert , Agbandje-McKenna Mavis 
Published: May 2021

AbstractRecombinant Adeno-associated viruses (rAAVs) are one of the most commonly used vectors for a variety of gene therapy applications. In the last two decades research focused primarily on the characterization and isolation of new cap genes resulting in hundreds of natural and engineered AAV capsid variants while the rep gene, the other major AAV open reading frame, has been less studied. This is due to the fact that the rep gene from AAV serotype 2 (AAV2) enables the ssDNA packaging of recombinant genomes into most AAV serotype and engineered capsids. However, a major byproduct of all vector productions is empty AAV capsids, lacking the encapsidated vector genome, especially for non-AAV2 vectors. Despite the packaging process being considered the rate-limiting step for rAAV production, none of the rep genes from the other AAV serotypes have been characterized for their packaging efficiency. Thus, in this study AAV2 rep was replaced with the rep gene of a select number of AAV serotypes. However, this led to a lowering of capsid protein expression, relative to the standard AAV2-rep system. In further experiments the 3’end of the AAV2 rep gene was reintroduced to promote increased capsid expression and a series of chimeras between the different AAV Rep proteins were generated and characterized for their vector genome packaging ability. The utilization of these novel Rep hybrids increased the percentage of genome containing (full) capsids ~2-4-fold for all of the non-AAV2 serotypes tested. Thus, these Rep chimeras could revolutionize rAAV production.ImportanceA major byproduct of all Adeno-associated virus (AAV) vector production systems are “empty” capsids, void of the desired therapeutic gene, and thus do not provide any curative benefit for the treatment of the targeted disease. In fact, empty capsids can potentially elicit additional immune responses in vivo gene therapies if not removed by additional purification steps. Thus, there is a need to increase the genome packaging efficiency and reduce the number of empty capsids from AAV biologics. The novel Rep hybrids from different AAV serotypes described in this study are capable of reducing the percentage of empty capsids in all tested AAV serotypes and improve overall yields of genome-containing AAV capsids at the same time. They can likely be integrated easily into existing AAV manufacturing protocols to optimize the production of the generated AAV gene therapy products.
Alternatives to mental health admissions for children and adolescents experiencing mental health crises: a systematic review of the literature
Authors: Clisu Denisa , Layther Imogen , Dover Deborah , Viner Russell M. , Read Tina , Cheesman David , Hodges Sally , Hudson Lee D 
Published: May 2021

AbstractBackgroundIncreasingly more children and young people (CYP) present in mental health crises, many being hospitalised due to concerns around illness severity and lack of community services. To release the burden of admission, we systematically reviewed the literature on the effects of proposed alternatives to CYP in crises.MethodsThree databases (PsychInfo, PubMed and Web of Science) were searched for peer-reviewed papers in October 2020, with an updated search in May 2021.ResultsWe identified 19 papers of interventions delivered in the emergency department, the home, outside of home but outside of clinics and in hospital clinics. The best evidence came from in-home interventions, in particular multisystemic therapy (MST), which proved to be promising alternatives by improving psychological outcomes and decreasing length of inpatient stay. The quality of included studies was low, with less than half being randomised controlled trials and only half of these at low risk of bias.ConclusionsWe could not recommend a particular intervention as an alternative to inpatient admission, however our review describes benefits across a range of types of inteventions that might be considered in multi-modal treatments. We also provide recommendations for future research, in particular the evaluation of new interventions as they emerge.
Detecting the ecological footprint of selection
Authors: Luiselli Juliette , Overcast Isaac , Rominger Andrew , Ruffley Megan , Morlon Hélène , Rosindell James 
Published: May 2021

AbstractThe structure of communities is influenced by many ecological and evolutionary processes, but the way this manifests in classic biodiversity patterns often remains unclear. Here, we aim to distinguish the ecological footprint of selection – through competition or environmental filtering – from that of neutral processes that are invariant to species identity. We build on existing Massive Eco-evolutionary Synthesis Simulations (MESS), which uses information from three biodiversity axes – species abundances, genetic diversity, and trait variation – to distinguish between mechanistic processes. In order to correctly detect and characterise competition, we add a new and more realistic form of competition that explicitly compares the traits of each pair of individuals. Our results are qualitatively different to those of previous work in which competition is based on the distance of each individual’s trait to the community mean. We find that our new form of competition is easier to identify in empirical data compared to the alternatives. This is especially true when trait data are available and used in the inference procedure. Our findings hint that signatures in empirical data previously attributed to neutrality may in fact be the result of pairwise-acting selective forces. We conclude that gathering more different types of data, together with more advanced mechanistic models and inference as done here, could be the key to unravelling the mechanisms of community assembly.
High-dose drug heat map based on organoid array chip for drug selection with high safety and efficacy
Authors: Lee Sang-Yun , Teng Yvonne , Son Miseol , Ku Bosung , Moon Ho Sang , Tergaonkar Vinay , Chow Pierce Kah-Hoe , Lee Dong Woo , Nam Do-Hyun 
Published: May 2021

ABSTRACTAn organoid array chip was developed by adopting a micropillar and microwell structure to test safety and efficacy of drugs using high dose drug heat map. In the chip, we encapsulated patient-derived cells in alginate and grow them to maturity for more than 7 days to form cancer organoids. When screening drug compounds in a high-density organoid array due to lack of number of patient-derived cells, changing media without damage of organoids is a very tedious and difficult process. Organoids grown in conventional well plates needed too many cells and were also easily damaged due to multiple pipetting during maintenance culture or during experimental procedures. To solve those problem, we applied a micropillar and microwell structure to the organoid array. We used patient-derived cells from patients with Glioblastoma multiforme (GBM), the most common and lethal form of central nervous system cancer, to validate the array chip performance. After forming more than 100µm-diameter organoids in 12 × 36 pillar array chip (25mm × 75mm), we tested 70 drug compounds (6 replicates) with high high-dose to find out high safety and efficacy drug candidates. Comparing the drug response of organoids derived from normal cells and cancer cells, we identified four compounds (Dacomitinib, Cediranib, Ly2835219, BGJ398) as drug candidates without toxicity to GBM cells.