bluuebunny/crossref_metadata_2025_split huggingface dataset:
Distinct mitochondrial remodeling during early cardiomyocyte development in a human-based stem cell model
Authors: Mostafavi Sepideh , Balafkan Novin , Nitschke Pettersen Ina Katrine , Nido Gonzalo S. , Siller Richard , Tzoulis Charalampos , Sullivan Gareth , Bindoff Laurence A. 
Published: July 2021

AbstractGiven the considerable interest in using stem cells for modelling and treating disease, it is essential to understand what regulates self-renewal and differentiation. Remodeling of mitochondria and metabolism, with the shift from glycolysis to oxidative phosphorylation (OXPHOS), play a fundamental role in maintaining pluripotency and stem cell fate. It has been suggested that metabolic ‘switch’ from glycolysis to OXPHOS is germ-layer specific as during early ectoderm commitment, glycolysis remains active while during the transition to mesoderm and endoderm lineages, it is downregulated. How mitochondria adapt during these metabolic changes and whether mitochondria remodeling is tissue specific remains unclear. Here we address the question of mitochondrial adaption by examining the differentiation of human pluripotent stem cells to cardiac progenitors and further to functional cardiomyocytes. Contrary to recent findings in neuronal differentiation, we found that mitochondrial content decreases continuously during mesoderm differentiation, despite clear mitochondrial remodeling giving increased mitochondrial activity and higher levels of ATP-linked respiration. Thus, our work both highlights similarities in mitochondrial remodeling during the transition from pluripotent to multipotent state in ectodermal and mesodermal lineages, while at the same time demonstrating cell-lineage-specific adaptions upon further differentiation. Our results improve understanding of how mitochondria remodeling and the metabolism interact during differentiation and show that it is erroneous to assume that increased OXPHOS activity during differentiation requires a simultaneous expansion of mitochondrial content.Summary statementWe found that mitochondrial content decreases continuously during mesoderm differentiation, despite clear mitochondrial remodeling giving increased mitochondrial activity and higher levels of ATP-linked respiration during mesoderm differentiation.
S18-phosphorylation of USP7 regulates interaction with TCEAL4 that defines specific complexes and potentially distinct functions
Authors: Querques Francesca , Darling Sarah , Cheetham-Wilkinson Izaak , Kim Robbert Q , Hapangama Dharani K , Sixma Titia K , Coulson Judy M 
Published: July 2021

AbstractUSP7 is a nuclear deubiquitylase (DUB) with multiple cancer-associated substrates for which selective inhibitors are available, yet it remains unclear how the pleiotropic effects of USP7 are regulated. We report that S18-phosphorylation does not influence USP7 catalytic activity but instead confers selectivity for protein interactions. In particular, non-S18-phosphorylatable USP7 preferentially interacts with USP11 and TRIM27, together with TCEAL1 and TCEAL4 whose functions are unknown. Intriguingly, USP7 can interact with two cellular forms of TCEAL4, but USP11 only interacts with a lower abundance K142 mono-ubiquitylated form (TCEAL4-Ub), which can scaffold a complex containing both DUBs. Whilst USP11 and TCEAL4 are both USP7 substrates, TCEAL4-Ub levels are specifically maintained by USP11 with their levels positively correlated in cancer cell lines. Together these data illustrate how USP7 phosphorylation and TCEAL4 ubiquitylation combine to define distinct USP7 complexes. As TCEAL4 itself interacts with proteins involved in ubiquitylation and various forms of DNA regulation, these complexes may direct cellular activity of USP7.
NGF-p75 signaling coordinates skeletal cell migration during bone repair
Authors: Xu Jiajia , Li Zhao , Tower Robert J. , Negri Stefano , Wang Yiyun , Meyers Carolyn A. , Sono Takashi , Qin Qizhi , Lu Amy , Xing Xin , McCarthy Edward F. , Clemens Thomas L. , James Aaron W. 
Published: July 2021

AbstractBone regeneration following injury is initiated by inflammatory signals and occurs in association with infiltration by sensory nerve fibers. Together, these events are believed to coordinate angiogenesis and tissue reprogramming, but the mechanism of coupling immune signals to re-innervation and osteogenesis is unknown. Here, we found that NGF is expressed following cranial bone injury and signals via p75 in resident mesenchymal osteogenic precursors to impact their migration into the damaged tissue. Mice lacking Ngf in myeloid cells demonstrated reduced migration of osteogenic precursors to the injury site with consequently delayed bone healing. These features were phenocopied by mice lacking p75 in Pdgfra+ osteoblast precursors. Single-cell transcriptomics identified mesenchymal subpopulations with potential roles in cell migration and immune response, altered in the context of p75 deletion. Together, these results identify the role of p75 signaling pathway in coordinating skeletal cell migration during early bone repair.
Integrated treatment of dual disorders in The Netherlands: a qualitative study of client experiences
Authors: Wamel Anneke van , Lempens Ankie , Neven Arjen 
Published: July 2021

PurposeMany clients who suffer from severe mental health problems also struggle with alcohol and drug use. And although there seems to be a consensus in most European countries that integrated treatment models (especially the integrated treatment of dual disorder clients model) are best fitted to help these clients, none of these have strong evidence, mostly owing to methodological limitations in the studies. This study aims to contribute to the knowledge of why integrated treatment is helpful by conducting in-depth interviews with dual disorder clients who are currently receiving integrated treatment.Design/methodology/approachThis study is a qualitative thematic analysis using a timeline tool to elicit participants’ responses. In the interviews, dual diagnosis clients with severe problems reflected on their situation at admission, the care they received in integrated treatment and their opinions on positive and negative elements. The interviews were coded and analysed with MAXQDA.FindingsIn the analyses of the interviews, this study found three clusters of elements that clients indicated were the most helpful during their treatment and recovery process: trusting and meaningful relationship with the team, components of integrated treatment and organisation of care.Originality/valueThe findings emphasized the importance of working relationship in the treatment of complex and long-term problems. This has implications for the evaluation of these integrated treatment models which so far mainly focuses on specific interventions.
Self-efficacy, habit strength, health locus of control and response to the personalised nutrition Food4Me intervention study
Authors: J Stewart–Knox Barbara , Rankin Audrey , P Bunting Brendan , J Frewer Lynn , Celis-Morales Carlos , M Livingstone Katherine , Fischer Arnout R.H. , Poínhos Rui , Kuznesof Sharron , J Gibney Mike , Mathers John C. 
Published: July 2021

PurposeRandomised controlled trials identify causal links between variables but not why an outcome has occurred. This analysis sought to determine how psychological factors assessed at baseline influenced response to personalised nutrition.Design/methodology/approachWeb-based, randomised, controlled trial (RCT) was conducted across seven European countries. Volunteers, both male and female, aged over 18 years were randomised to either a non-personalised (control) or a personalised (treatment) dietary advice condition. Linear mixed model analysis with fixed effects was used to compare associations between internal and external health locus of control (HLoC), nutrition self-efficacy (NS-E) and self-report habit index (S-RHI) at baseline (N = 1444), with healthy eating index (HEI) and Mediterranean diet index (MDI) scores between conditions post-intervention (N = 763).FindingsAn increase in MDI scores was observed between baseline and six months in the treatment group which was associated with higher NS-E (p < 0.001), S-RHI (p < 0.001) and external HLoC (p < 0.001). Increase in HEI between baseline and six months in the treatment group was associated with higher NS-E (p < 0.001) and external HLoC (p = 0.009). Interaction between time and condition indicated increased HEI scores (p < 0.001), which were associated with higher S-RHI scores in the treatment than control group (p = 0.032). Internal HLoC had no effect on MDI or HEI.Originality/valuePsychological factors associated with behaviour change need consideration when tailoring dietary advice. Those with weaker habit strength will require communication focussed upon establishing dietary habits and support in integrating advised changes into daily routine. Information on habit strength can also be used to inform how progress towards dietary goals is monitored and fed back to the individual. Those with stronger habit strength are more likely to benefit from personalised nutrition.
Strengthening the role of communication departments: A framework for positioning communication departments at the top of and throughout organizations
Authors: Brockhaus Jana , Zerfass Ansgar 
Published: July 2021

PurposeCorporate communications is often less successful when it is competing for influence with neighboring functions such as marketing or sales within organizations. This article addresses the internal positioning of communication departments by developing a conceptual framework which helps to understand, analyze and optimize their standing in organizations.Design/methodology/approachThe research is based on a literature review across several disciplines (e.g. organizational communication, strategic management) and supported by 26 qualitative in-depth interviews with board members, executives and communicators in a global industry company. By combining the theoretical and empirical insights, a framework for positioning communication departments within organizations was developed.FindingsThe framework depicts seven strategies (e.g. expectation and impression management, supporting ambassadors from other departments) and three spheres of influence (organizational integration, internal perceptions and social capital) to strengthen the position of corporate communications.Research limitations/implicationsThe conceptual framework has been supported by one case study so far, and future research may further develop and verify it by applying it to a larger number of companies in different industries.Practical implicationsPractitioners can use the framework as an analytical tool to reflect the current situation in their organization and identify opportunities for strengthening it.Originality/valueThis article introduces a novel view in the academic debate about the role and influence of corporate communications. It establishes a framework that helps to identify different drivers and strategies, and lays ground for future research.
Shared leadership and innovative behavior in scientific research teams: a dual psychological perspective
Authors: Liu Hongbo , Gao Suying , Xing Hui , Xu Long , Wang Yajie , Yu Qi 
Published: July 2021

PurposeThe purpose of this study is to investigate the mechanism of shared leadership on team members’ innovative behavior.Design/methodology/approachPaired questionnaires were collected from 89 scientific research teams in the Beijing-Tianjin-Hebei region of China at two-time points with a time lag of 4 months. Then multilevel structural equation model method was applied to analyze the multiple mediating effects.FindingsThis study finds that: the form of shared leadership in scientific research teams of universities; shared leadership has a positive impact on team members’ innovative behavior; there are multiple mediations in the relationship including synchronization and sequence of creative self-efficacy and achievement motivation.Originality/valueAccording to the “stimulus-organism-response” model, this paper has constructed a multi-level theoretical model that shared leadership influences individual innovation behavior and reveals the “black box” from the perspective of psychological mechanism. It not only verifies that “can-do” shapes “willing to do” but also makes up for the gap of an empirical test of the effectiveness of shared leadership in scientific research teams of universities. Besides, the formal scale of shared leadership in the Chinese situation is revised, which can provide a reference for future empirical research on shared leadership. The research conclusions provide new ideas for improving the management of scientific research teams in universities.
‘Big data’ patentometrics for R&D decision-making
Authors: Verma Charu , Suri Pradeep Kumar 
Published: July 2021

Purpose
The purpose of this paper is to highlight the use of big data through patentometric insights for R&D decision-making.

Design/methodology/approach
This study assesses the inventive activity through ‘big data’ patents, registered by inventors worldwide, using WIPO Patentscope database. The objective is to use the insights from patentometrics for R&D decision-making. The data from WIPO PatentScope (https://patentscope.wipo.int/search/en/search.jsf) was searched for current patent scenario in area of ‘big data’. The data was further organized and cleaned using the Google ‘OpenRefine’. Data was pre-processed to remove all null values. Cleaned data was analyzed using programming language ‘R’, MS Excel (charts and Pivot tables) and free data visualization tool called ‘Tableau Public’, to get insights for R&D decision-making.

Findings
The key insights included trends (patents with years of publication), top technologies trending the current space, top organizations leading in these technologies and the top inventors who are publishing patents in these technologies through leading organizations were drawn. Details in Section 5 in the paper.

Research limitations/implications
Global patent data is multi-lingual and spreads across a set of multiple databases. Domain experts may be required to assess, identify and extract the relevant information for analysis and visualization of multi-lingual distributed data sets. Government organizations generally have multi-dimensional goals that may be more toward societal benefits. On the other hand, the commercial companies are more focused on profit. Therefore, the performance management process has to be really effective because it is critical for getting value in the government sector.

Practical implications
Insights from patent analytics serve as the important input to R&D managers as well as policymakers to assess the global needs to plan the national orientation according to the global market. This will help further for R&D projects prioritization, planning, budget allocations, human capital planning and other gamut of R&D management and decision-making.

Social implications
Facilitation for R&D institutions (government as well as private) to formulate the research strategy for the domains or research areas to delve into. R&D decisions will be completely data-driven making them more accurate, reliable, valid and informed. These insights are very relevant for policymakers as well to facilitate the need assessment to determine the National priorities, make improvements in meeting societal country-level challenges during the resource allocation at top and subsequently at all other levels.

Originality/value
Data analytics of global patents in “big data” till 2019 to get insights to facilitate R&D decision-making.
Assessing the integration of climate change adaptation and mitigation into national development planning of Ethiopia
Authors: Hirpha Hurgesa Hundera , Mpandeli Sylvester , Bantider Dagnew Amare , Chibsa Temesgen , Abebe Cherinet 
Published: July 2021

Purpose
Policy framework has significant roles in minimizing the impact of climate change in agrarian societies like Ethiopia. The purpose of this paper is to assess the integration of issues related to climate change adaptation into the national development planning of Ethiopia.

Design/methodology/approach
A qualitative research design, which depended on secondary and primary data sources, was used in this study. Data were collected from relevant documents. These were substantiated with field data gathered through key informant interviews and focus group discussions from participants identified using purposive sampling. Thematic analysis of the collected data was done by first considering the relevant documents and then comparing with the field data. During the analysis and interpretation, the results were combined to explain, confirm, refute and/or enrich the data obtained through document reviews and interviews.

Findings
The result of the study revealed that the general issues of environmental management have been included in the relevant documents (national plans). However, the documents do not explicitly identify climate change adaptation strategies and options that can alleviate the current impacts and the projected negative impacts associated with climate change. In fact, some documents were found to be characterized by numerous gaps. For example, the environmental policy of Ethiopia does not address climate trading, climate resilient green economy and recent development. The result from interviews shows that the constitution of the country lacks sufficiently addressing climate change adaptation. The result obtained from focus group discussion with informants indicated that the environmental policy of the country is shallow and suffers from showing clear direction regarding integration. The informants indicate that though there is an office that works on climate change at zonal level, there is no well-defined structure for climate change at zonal, district and Kebele levels and there appears to be weak integration among the different institutions working on climate change.

Originality/value
This study would speed up the revision of environmental policy of Ethiopia and the development of a separate policy document that focuses on adaptation to climate change.
Employees’ challenge-hindrance appraisals toward STARA awareness and competitive productivity: a micro-level case
Authors: Ding Li 
Published: July 2021

PurposeThis study aims to examine the effect of restaurant employees’ challenge-hindrance appraisals toward smart technology, artificial intelligence, robotics and algorithms (STARA) awareness on individual competitive productivity (ICP) and explore the mediating roles of employees’ work engagement and organizational commitment on the relationship between challenge-hindrance appraisals and ICP.Design/methodology/approachData were collected through an online survey. One hundred and ninety employees who worked at full-time and non-management positions in the USA quick-service restaurants participated. Partial least squares structural equation modeling was used for the data analysis.FindingsThe study identified that restaurant employees’ challenge appraisals toward STARA awareness positively influenced ICP. This relationship is positively mediated by employees’ work engagement.Practical implicationsThis study makes practical contributions to human resource practices in restaurants. Employees’ challenge appraisals toward STARA awareness transmit the job insecurity stressor to a higher level of ICP. Restaurant managers should provide employees with adequate resources and support for non-management employees’ professional competency growth. Quick-service restaurants can enjoy a competitive advantage in the market by enhancing employees’ CP.Originality/valueThis study enriches the literature on the CP model, cognitive appraisal theory and person-environment fit theory. The study investigated employees’ challenge and hindrance appraisals toward emerging STARA awareness and emphasized their distinct characteristics to drive ICP in the quick-service restaurant sector.
Have coffee/tea, will travel: assessing the inclination towards sustainable coffee and tea tourism among the green generations
Authors: Yeap Jasmine A.L. , Ooi Say Keat , Ara Husna , Said Muhamad Faizal 
Published: July 2021

Purpose
This study aims to identify the key variables which determine intentions to visit coffee/tea tourism plantations particularly those adopting sustainable practices. Also, this study ascertained the perception of risk in travelling due to the fear of Covid-19 on travel intentions to such coffee/tea tourism destinations.

Design/methodology/approach
Using the theory of planned behaviour as a basis for this study’s framework, data was gathered from 302 eco-conscious Generation Y and Z consumers via an online survey. Partial least squares were then applied to analyse the data.

Findings
Learning and relaxation motives were important in determining consumers’ attitudes towards sustainable coffee/tea tourism. The intention to engage in sustainable coffee/tea tourism is most strongly affected by the risk of travelling, followed by attitude.

Research limitations/implications
The addition of contemporary variables was given to the theory of planned behaviour’s core constructs to better reflect consumers’ attitude and behaviour towards a growing form of tourism under unprecedented times.

Practical implications
Travel or tourism operators will have first-hand insights on the factors that drive intentions to visit sustainable coffee and tea destinations, thus enabling more strategic action to be undertaken to reach the targeted young consumers.

Originality/value
This study examines young, environmental-conscious consumers’ perspectives on novel travel destinations which adopt sustainable practices. Risk in travelling was assessed which is necessary given Covid-19 has severely disrupted consumers’ travel patterns.
Transition to work, mismatch and underemployment among graduates: an Australian longitudinal study
Authors: Jackson Denise , Li Ian 
Published: July 2021

PurposeThere are ongoing concerns regarding university degree credentials leading to graduate-level employment. Tracking graduate underemployment is complicated by inconsistent measures and tendencies to report on outcomes soon after graduation. Our study explored transition into graduate-level work beyond the short-term, examining how determining factors change over time.Design/methodology/approachWe considered time-based underemployment (graduates are working less hours than desired) and overqualification (skills in employment not matching education level/type) perspectives. We used a national data set for 41,671 graduates of Australian universities in 2016 and 2017, surveyed at four months and three years' post-graduation, to explore determining factors in the short and medium-term. Descriptive statistical techniques and binary logistic regression were used to address our research aims.FindingsGraduates' medium-term employment states were generally positive with reduced unemployment and increased full-time job attainment. Importantly, most graduates that were initially underemployed transited to full-time work at three years post-graduation. However, around one-fifth of graduates were overqualified in the medium-term. While there was some evidence of the initially qualified transitioning to matched employment, supporting career mobility theory, over one-third remaining overqualified. Skills, personal characteristics and degree-related factors each influenced initial overqualification, while discipline was more important in the medium-term.Originality/valueOur study explores both time-based underemployment and overqualification, and over time, builds on earlier work. Given the longer-term, negative effects of mismatch on graduates' career and wellbeing, findings highlight the need for career learning strategies to manage underemployment and consideration of future labour market policy for tertiary graduates.