bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluation of an effective detection and quantification method for particular microorganisms by comparing NGS-based metagenome profiling data
Authors: Han Kyudong , Jeong Jinuk , Mun Seyoung , Oh Yunseok , Cho Chun-Sung , Yun Kyeongeui , Ahn Yongju , Chung Won-Hyong , Lim Mi Young , Hwang Tae Soon 
Published: June 2021

Abstract
Metagenome profiling research using next-generation sequencing (NGS), a technique widely used to analyze the diversity and composition of microorganisms living in the human body, especially the gastrointestinal tract, has been activated, and there is a growing interest in the quantitative and diagnostic technology for specific microorganisms. According to recent trends, quantitative real-time PCR (qRT-PCR) are still of considerable technique in detecting and quantifying bacteria associated with the human mouth, nasal cavity, and pharynx due to analytical cost and time burden of NGS technology. Here, based on NGS metagenome profiling data produced by utilizing 100 gut microbiota samples, we conducted a comparative analysis of identifying for five bacterial genera proportions (Akkermansia, Bacteroides, Bifidobacterium, Phascolarctobacterium, and Roseburia) within same metagenomic DNA samples through qRT-PCR assay in parallel. Genus-specific primer, targeting the particular gene of each genus for qRT-PCR assay, allowed a statistically consistent quantification pattern with the metagenome profiling data. Furthermore, results of bacterial identification through Sanger validation demonstrated the high genus-specificity of each primer set. Therefore, our study suggests that an approach to quantifying specific microorganisms by applying qRT-PCR method can compensate for the concerns (potential issues) of NGS while also providing efficient benefits to various microbial industries
Detection of potentially pathogenic Arcobacter spp. in Bangkok canals and the Chao Phraya River
Authors: Tomioka Noriko , Yoochatchaval Wilasinee , Takemura Yasuyuki , Matsuura Norihisa , Danshita Tsuyoshi , Srisang Pornpawee , Mungjomklang Noppamas , Syutsubo Kazuaki 
Published: June 2021

Abstract
The management of pathogenic bacteria in waterways is a public health issue. Here, we investigated the concentrations of potentially pathogenic bacteria, Arcobacter spp. and Campylobacter spp., and Escherichia coli, by quantifying species-specific genes in surface water samples from canals and the Chao Phraya River from June 2017 to June 2018 in Bangkok, Thailand. We assessed the relationship between the specific bacterial concentrations, water quality, and seasonal changes. Arcobacter spp. were detected at high density in all samples and showed seasonal fluctuations according to analyses based on 16S rDNA and the invasion gene ciaB. High levels of 16S rDNA and dut gene of E. coli were detected in the polluted drainage canals. A high correlation was observed between E. coli and chemical and biochemical oxygen demand (COD and BOD), suggesting that untreated domestic wastewater was the source of the E. coli. In contrast, Arcobacter spp. were detected with high density even in water samples with relatively low COD, suggesting that Arcobacter spp. are more likely than E. coli to survive in the water environment. The analysis of 16S rDNA and ciaB gene sequence analyses indicated that the Arcobacter spp. isolated from the drainage canals were A. butzleri and A. cryaerophilus.
Design and Development of a Solar Water Purification System with
Graphene-Plasmonic Based Hybrid Nanocomposites: A Review
Authors: Javed Hafiz Muhammad Asif , Sarfaraz Muhammad , Mahmood Asif , Nisar M. Zubair , Qureshi Akbar Ali , Khan Muhammad Azhar , Zhong Peng , Liao Yulong 
Published: June 2021

Abstract:
Harvesting solar energy for water treatments has been considered a promising solution
for a global community. The shortage of water is a great challenge for scientists due to the increased
demand of the population–however, the low efficiency of absorber materials obstacles in
practical applications. In addition, state-of-the-art conventional technologies require optical concentrators
and multiple component-based systems, leading to lower efficiency and higher cost. In
this review, a low-cost, more reliable, less energy-intensive, and more eco-friendly solar water purification
system based on graphene-plasmonic hybrid nanocomposite has been demonstrated.
Graphene-plasmonic-based hybrid nanocomposite has been utilized to achieve pure water from
wastewater. Such hybrid nanocomposites have the ability to clean polluted water very efficiently
due to their excellent properties such as higher surface area, low concentration, and working ability.
Furthermore, the development of a solar water purification system has been achieved through
optimized hybrid nanocomposites.
COVID-19 Improved Diagnoses Based on the Open-morphology Filter and
Deep-learning
Authors: Malallah Fahad L. , Younus Majid D. , Zedan Mohammad J.M. , Saeed Mustafa G. 
Published: June 2021

Background:
Coronavirus (COVID-19) has appeared first time in Wuhan, China, as an
acute respiratory syndrome and spread rapidly. It has been declared a pandemic by the WHO. Thus,
there is an urgent need to develop an accurate computer-aided method to assist clinicians in identifying
COVID-19-infected patients by computed tomography CT images. The contribution of this paper
is that it proposes a pre-processing technique that increases the recognition rate compared to the
techniques existing in the literature.

Methods:
The proposed pre-processing technique, which consists of both contrast enhancement and
open-morphology filter, is highly effective in decreasing the diagnosis error rate. After carrying out
pre-processing, CT images are fed to a 15-layer convolution neural network (CNN) as deep-learning
for the training and testing operations. The dataset used in this research has been publically published,
in which CT images were collected from hospitals in Sao Paulo, Brazil. This dataset is composed
of 2482 CT scans images, which include 1252 CT scans of SARS-CoV-2 infected patients
and 1230 CT scans of non-infected SARS-CoV-2 patients.

Results:
The proposed detection method achieves up to 97.8% accuracy, which outperforms the reported
accuracy of the dataset by 97.3%.

Conclusion:
The performance in terms of accuracy has been improved up to 0.5% by the proposed
methodology over the published dataset and its method.
Zastosowanie metody biocenotycznej oceny drzew w parku „Willa Mindic” (Mołdawia) na potrzeby wykonania projektu koncepcyjnego zagospodarowania terenu – studium przypadku
Authors: Ozimkowska Lidia , Wojtatowicz Jerzy 
Published: June 2021

W artykule przedstawiono zastosowanie metody oceny biocenotycznej drzew w parku „Willa Mindic” (Mołdawia). Przeprowadzając inwentaryzację uwzględniano wstępne decyzje projektowe, które także miały wpływ na określenie wartości drzew. Rozszerzając waloryzację roślin o badanie stanu zdrowotnego, w tym bezpieczeństwa dla ludzi, uzyskano wyniki, które służyły do wykonania projektu. Wytypowano miejsca, które bez względu na warunki muszą zapewnić bezpieczne przebywanie, fragmenty terenu, na których zwiedzanie parku jest ograniczone oraz te, na których ze względu na wysokie wartości biocenotyczne, w tym ochronę miejsc lęgu i rozrodu zwierząt, nie przewiduje się ciągów komunikacyjnych i miejsc wypoczynkowych. Na podstawie badań wskazano obszary o wyjątkowym znaczeniu dla zwierząt oraz wykonano projekt koncepcyjny zagospodarowania terenu uwzględniający potrzeby wypoczynkowe ludzi.
PERANAN PUPUK DAN FUNGISIDA TERHADAP PEMULIHAN TAJUK AKIBAT PENYAKIT GUGUR DAUN PESTALOTIOPSIS PADA KLON GT 1 DI KEBUN PERCOBAAN PUSAT PENELITIAN KARET SEMBAWA
Authors: Febbiyanti Tri Rapani , Stevanus Charlos Togi , Tistama Radite 
Published: June 2021

Penyakit gugur daun Pestalotiopsis (PGDP mengalami outbreak dengan luas serangan di Indonesia mencapai 382.000 ha pada tahun 2019. Akibatnya, penurunan produksi karet lebih dari 30%. Hingga saat ini, cara yang efektif untuk pengendalian penyakit ini belum dilaporkan. Upaya pengendalian penyakit dapat dilakukan dengan meningkatkan sistem pertahanan tanaman dan menekan jumlah inokulum di perkebunan. Penelitian ini bertujuan untuk mengetahui pengaruh aplikasi pupuk dan fungsida terhadap kerapatan tajuk dan penambahan produksi pada klon GT 1 yang terserang PGDP. Dibandingkan kontrol, pemupukan 100 % dosis rekomendasi meningkatkan kerapatan tajuk 4% dengan kenaikan produksi 3 g/p/s. Pemberian ekstra 25% Nitrogen dan ekstra Kalium dapat memicu kerapatan tajuk berturut-turut 5% dan 6% dengan kenaikan produksi 5 dan 6 g/p/s. Kerapatan tajuk terbukti meningkat baik dengan penyemprotan fungisida di gawangan maupun pengasapan pada daun muda masing-masing 9%, dan diiringi kenaikan produksi berturut-turut 5 g/p/s. Kombinasi aplikasi pupuk ektra 25% N, K dan fungisida pada gawangan dan fogging memberikan kerapatan tajuk 9% dan kenaikan produksi 10 g/p/s. Pemupukan berperan dalam meningkatkan tajuk dan produksi. Aplikasi fungisida di gawangan dan fogging dapat mempertahankan tajuk lebih baik.
Еffectiveness and safety of the in vitro fertilization program for HIV infection in men
Authors: Mityurina E. V. , Perminov S. G. , Ushakova I. V. , Kozyrin N. V. , Kravchenko A. V. 
Published: June 2021

Purpose of the study:to evaluate the effectiveness and safety of IVF/ICSI program for couples with HIV infected men.Research and methods.Prospective clinical study was applied to 169 infertile married couples. The main group of subjects was 94 couples with HIV infected men. The control group included 75 couples with HIV negative status for both partners. In the main group there were carried out 60 treatment IVF/ICSI cycles and 31 frozen protocols, in the control group 86 and 39 cycles respectively. Before the Assisted Reproductive Technology (ART) was applied to the HIV infected men they were tested for DNA HIV in purified spermatozoa by PCR method. To evaluate the seroconversion in 21 days following the embryo transfer the women were tested for the presence of an antigen/antibodies to the HIV virus.Results.Clinical pregnancy rate [6 (19,4%) and 17 (27%); p=0,41] as well as birth rate [5 (16,1%) and 11 (17,5%); p=0,87] in couples with HIV infected male was comparable to the control group. The testing of semen samples showed no RNA HIV in 100% cases. Evaluation of seroconversion showed absolutely no antibodies to HIV in the women’s blood in 100% cases.Conclusion.Implementation of the IVF/ICSI program for discordant married couples with HIV infected male showed the rate of pregnancy onset which is comparable to the HIV seronegative control. Ejaculate processing of HIV infected male using «double» gradient and swim up is turns to be the measure to prevent horizontal transmission of the virus. This method can be recommended when implementing ART for couples with HIV infected male, both in the case of infertility and according to epidemiological indications.
A Constituição de 1988 e a reforma política no Brasil: Lições de quatro momentos de votação
Authors: Nogueira Andrade Eric 
Published: June 2021

Eleições são o alicerce de toda democracia representativa. Curiosamente, grande parte dos políticos brasileiros demonstram grande insatisfação com as regras eleitorais vigentes no país desde a redemocratização em 1988 e propõem recorrentemente um conjunto de propostas de alterações. É sabido que o sistema eleitoral de uma democracia não se altera com facilidade. Uma combinação de elementos precisa estar presente. No caso brasileiro as propostas de modificar o sistema eleitoral foram em quatro momentos sistematicamente rejeitadas, ainda que muitas outras mudanças na legislação eleitoral tenham sido aprovadas ao longo das sucessivas legislaturas. Para a compreensão desse processo, este trabalho focou parte do seu esforço inicial em apresentar duas possíveis abordagens teóricas que enxergam reformas a partir dos seus efeitos interpartidário e intrapartidário. Também foram objeto de análise quatro comissões especiais de reforma política que constituem a base dos debates sobre a reforma eleitoral na Câmara dos Deputados. Essas comissões propuseram e aprovaram importantes alterações na legislação eleitoral do Brasil. O exame comparativo dessas comissões pode lançar luz sobre parte dos questionamentos acerca das inúmeras alterações ocorridas. Por fim, examino quatro momentos em que uma alternativa ao sistema eleitoral foi posta em votação. Os resultados indicam que o longo processo de reforma no Brasil pós-1988 teve como grande objetivo a redistribuição da correlação de forças partidária, restringindo o acesso de pequenos partidos ao parlamento, e limitando, ainda que levemente, a personalização do sistema eleitoral, afetando diretamente a proporcionalidade dos resultados eleitorais das eleições de 2018. Também foi possível demonstrar que o sistema eleitoral não se alterou devido à clara falta de acordo entre os grandes partidos. A cada proposta votada, os maiores partidos se revezaram na liderança das coalizões que as vetaram. O artigo combina teoria, analise cronológica e comparação para alcançar os seus objetivos.
Palavras Chaves: Reforma política; Sistema eleitoral; Sistema partidário; estudos legislativos; Crise política.
Manajemen Asuhan Kebidanan pada Bayi Ny “H” dengan Hipotermi di Puskesmas Jumpandang Baru Makassar
Authors: Sarnah Sarnah , Firdayanti Firdayanti , Rahma Andi Sitti 
Published: June 2021

Hipotermi adalah suhu tubuh di bawah 36,5ºC dan rentan terjadi pada bayi baru lahir, dan bisa berakibat fatal misalnya hipoglikemia dan berlanjut menjadi kejang dan bahkan kematian. Penelitian ini bertujuan untuk melaksanakan Manajemen Asuhan Kebidanan pada bayi Ny. “H” dengan Hipotermi di Puskesmas Jumpandang Baru Makassar sesuai dengan 7 langkah Varney dan SOAP. Hasil dari studi kasus yang dilakukan pada bayi Ny “H” ditegakkan diagnosis dengan Hipotermi berdasarkan anamnesis dan pemeriksaan fisik. Pada bayi Ny “H” ditandai dengan tanda-tanda vital heart rate : 124 x/menit, pernafasan : 44 x/menit, dan suhu : 35,3ºC dimana keadaan tubuh bayi dingin, refkles menghisap lemah, bibir pucat, pergerakan kurang aktif, dan kuku pucat. Pada pasien ini dilakukan penatalaksanaan yang bertujuan mengatasi hipotermi, mencegah komplikasi hipoglikemia, dan menjamin nutrisi dengan cara menjaga kehangatan bayi dengan bedong, mengganti pakaian atau popok bayi tiap kali basah, menganjurkan kepada ibu untuk menyusui bayinya secara on demand , mengobservasi tanda-tanda vital setiap pagi dan sore. Berdasarkan hasil dari studi kasus 7 langkah varney dan SOAP yang digunakan untuk proses penyelesaian masalah hipotermi pada nenonatus, diperoleh hasil evaluasi suhu badan bayi kembali normal dan tidak mengalami komplikasi yang dikhawatirkan.
Manajemen Asuhan Kebidanan Gangguan Sistem Reproduksi pada Ny “S” dengan Mioma Submukosa di RSUD Syekh Yusuf Gowa Tanggal 04 Februari – 25 Februari Tahun 2019
Authors: Astuti Astuti , Al Kautzar Anieq Mumthi’ah , Darmawansyih Darmawansyih 
Published: June 2021

Pendahuluan Mioma uteri adalah tumor jinak uterus yang mengandung sel-sel otot polos dan jaringan ikat dengan ciri bulat, keras, berwarna merah muda pucat. Umumnya berlokasi pada korpus uteri.Metode Jenis penelitian ini adalah studi kasus dengan pendekatan Manajemen Asuhan Kebidanan Gangguan Sistem Reroduksi pada Ny “S” dengan Mioma Submukosa di RSUD Syekh Yusuf Gowa Tahun 2019 sesuai Manejemen Asuhan Kebidanan 7 langkah Varney dan pendokumentasian dalam bentuk SOAP.Hasil Asuhan yang diberikan pada Ny “S” pada kasus mioma submukosa yaitu dilakukan dengan pemberian asuhan yang sesuai standar operasional serta melakukan upaya pencegahan komplikasi terjadinya torsi (putaran tungkai), infeksi, dan keganasan, serta dilakukan pemantauan dan asuhan manajemen kebidanan dilakukan pemantauan selama dua hari sebelum operasi dan tiga hari setelah operasi dan melakukan kunjungan rumah selama tiga kali.Kesimpulan dari kasus yaitu diantaranya pendokumentasian dalam bentuk SOAP yang digunakan untuk proses penyelesaian masalah kebidanan telah dilaksanakan pengkajian berupa pemantauan dari analisa data pada Ny “S” dengan Mioma Submukosa Di RSUD Syekh Yusuf Gowa Tahun 2019. Yakni dari pengkajian sebelum operasi dan setelah operasi tidak ditemukan komplikasi pada ibu di tandai dengan tanda-tanda vital normal, dan pengankatan tumor berjalan normal
Manajemen Asuhan Kebidanan Intranatal Berkelanjutan pada Ny “M” dengan Preeklampsia Berat di RSUD Syekh Yusuf Kabupaten Gowa Tanggal 15 Oktober – 21 November 2018
Authors: Kasnur Kasnur , Firdayanti Firdayanti , Rahmadhani Rauly 
Published: June 2021

Pendahuluan preeklampsia merupakan komplikasi kehamilan dan persalinan yang ditandai dengan peningkatan tekanan darah, proteinuria dan oedema, yang kadang-kadang disertai dengan komplikasi koma.Gejala dari preeklampsia seperti hipertensi, oedema dan proteinuria sering tidak diperhatikan, sehingga tanpa disadari dalam waktu singkat dapat timbul menjadi preeklampsia berat, bahkan eklampsia.Metode Jenis penelitian ini adalah studi kasus dengan pendekatan Manajemen Asuhan Kebidanan Intranatal Berkelanjutan pada Ny “M” dengan Preeklampsia Berat di RSUD Syekh Yusuf Kabupaten Gowa Tahun 2018 sesuai Manejemen Asuhan Kebidanan 7 langkah Varney dan pendokumentasian dalam bentuk SOAP.Hasil Penatalaksanaan Asuhan Persalinan dengan preeklampsia berat pada Ny “M” yaitu dilakukan pemberian asuhan yang sesuai standar operasional prosedur serta melakukan upaya pencegahan komplikasi dengan pemberian Magnesium Sulfat untuk mencegah terjadinya eklampsia, HELLP Sindrom, solusio plasenta, gangguan organ, KJDR pada bayi serta dilakukannya pemantauan dan Asuhan Manejemen Kebidanan dari kala I sampai kala IV.Kesimpulan dari kasus yaitu diantaranya pendokumentasian dalam bentuk SOAP yang digunakan untuk proses penyelesaian masalah kebidanan telah dilaksanakan pengkajian serta asuhan dengan penatalaksanaan yang sesuai pada Ny “M” dengan preeklampsia berat di RSUD Syekh Yusuf Kabupaten Gowa Tahun 2018 yakni dari kala I sampai kala IV, tidak ditemukannya komplikasi pada ibu dan bayi.
Program Peningkatan Keamanan Di Bandara Kualanamu Medan Apabila Beroperasi
Authors: Nadjamuddin Ismail 
Published: June 2021

Kualanamu Medan Airport Construction is an alternative to overcome the operational limitations and the land that was experienced Polonia Airport. Airport security enhancement program Kualanamu Medan when operating include airport security aspects of the provision of facilities consisting of: X-Ray facility 12 units, Walk Throught Metal Detector (WTMD) 14 units, Hand Held Metal Detector (HHMD) 24 units, Closed Circuit Television (CCTV , Explosive Detection System 2 units, 2 units Liquit Scan Detecto,, Detector Nubikara 2 units (Nuclear, Biological, Chemical, Radio Active), Body Scane 1 unit, Body Inspector, Airport For Perimeter Surveillance, Security Inspection Car and Motorcycle 4. The security officer in Medan Airport Kualanamu totaling 204 personnel consisting of 124 personnel from the PT. Angkasa PuraII, 40 BKO-military personnel who assisted and 40 staff personnel outsoursing. The system and airport security procedures will refer to the Regulation of the Minister of Transportation No. 9 of 2010 about the National Aviation Security Programme and ICAO in Annex17 on Security and Document-8973 on the Security Manual for Safeguarding Civil Aviation Against Acts of Unlawful Interference, that the safety and security systems at airports should be the maximum, using equipment and adequate procedures that ensure safety and smooth flight.
Pembangunan Bandara Kualanamu Medan merupakan alternatif untuk mengatasi keterbatasan operasional dan tanah yang dialami Bandara Polonia. Program peningkatan keamanan bandara Kualanamu Medan saat dioperasikan meliputi aspek keamanan bandara, penyediaan fasilitas yang terdiri dari: 12unit fasilitas X-Ray, 14 unit Walking Through Mental Detector (WTMD), 24 unit Hand Held Metal Detector (HHMD), 2 unit Closed Circuit Television (CCTV)dan sistem Deteksi peledak, 2 unit Liquit Pindai Detecto, 2 unit Detector Nubikara (Nuklir, Biologi, Kimia, Radio Aktif), Badan Scane 1 unit, Bandara Untuk Surveillance Perimeter, 4 Mobil Keamanan Inspeksi dan Sepeda Motor petugas keamanan di Medan Bandara Kualanamu sebesar 204 personel yang terdiri dari 124 personil dari PT Angkasa Pura II, 40 BKO-personil militer yang dibantu dan 40 personil staf outsoursing. Sistem dan prosedur keamanan bandara akan mengacu pada Peraturan Menteri Perhubungan Nomor 9 tahun 2010 tentang National Aviation Security Program dan ICAO dalam Annex-17 tentang Keamanan dan manual dokumen-8973 tentang Keamanan Penerbangan Sipil upaya pelanggaran hukum, bahwa keamanan dan sistem keamanan di bandara harus maksimal , menggunakan peralatan dan prosedur yang memadai yang menjamin keamanan dan kelancaran penerbangan.
Kebutuhan Kapasitas Fasilitas Land Side Untuk Tahun 2015 Sebagai Upaya Meningkatkan Kenyamanan Penumpang Di Bandar Udara Pattimura Ambon
Authors: Lukiana Lukiana 
Published: June 2021

In accordance to the Acceleration and Extension of Indonesian Economy Development (MP3EI) in Maluku from 2011 to 2025, PT (Persero) Angkasa Pura I is going to extend the terminals and aprons in Pattimura Airport, Ambon. The extension of passenger terminals is necessairily important because terminal area in an international airport has to be wide enough to improve passengers’ convenience along with the increasing passengers’ movement every year. In relation, in order to figure out the minimum land side capaicty needs of Pattimura Airport Ambon in 2015 that complies to the passengers’ movement development prediction in the same year, it is necessary to conduct a study on the landside facility needs as an effort to improve passengers’ convenience.From the sum result of passengers’ number at peak hour as predicted in 2015 which is 848 passengers, the minimum size of the departure hall area is 1993 m2, check in area is 297 m2, boarding room is 1329 m2 and baggage claim area is 845 m2.
Berdasarkan Masterplan Percepatan dan Perluasan Pembangunan Ekonomi Indonesia (MP3EI) di Maluku Tahun 2011-2025, PT. (Persero) Angkasa Pura I akan melakukan pengembangan terminal dan apron Bandar Udara Pattimura Ambon. Perluasan terminal penumpang sangat diperlukan karena sebagai bandar udara internasional, luas terminal akan meningkatkan kenyamanan penumpang mengimbangi meningkatnya jumlah pergerakan penumpang pertahun.terkait dengan hal tersebut di atas, untuk mengetahui kebutuhan kapasitas landside minimal Bandar Udara Pattimura Ambon pada tahun 2015 sesuai dengan perkiraan perkembangan pergerakan penumpang pada tahun tersebut, maka perlu dilakukan pengkajian terkait dengan kebutuhan fasilitas landside sebagai upaya meningkatkan kenyamanan penumpang.Dari hasil perhitungan dengan jumlah penumpang pada waktu sibuk yang diperkirakan pada tahun 2015 sebanyak 848 penumpang maka luas hall keberangkatan minimal 1993 m2 , check in area minimal 297m2 , luas ruang tunggu keberangkatan minimal 1329 m2 , luas baggage claim area minimal 845m2 , dan luas ruang tunggu keberangkatan minimal 1329 m2.