Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition)
Authors: Cossarizza Andrea , Chang Hyun‐Dong , Radbruch Andreas , Abrignani Sergio , Addo Richard , Akdis Mübeccel , Andrä Immanuel , Andreata Francesco , Annunziato Francesco , Arranz Eduardo , Bacher Petra , Bari Sudipto , Barnaba Vincenzo , Barros‐Martins Joana , Baumjohann Dirk , Beccaria Cristian G. , Bernardo David , Boardman Dominic A. , Borger Jessica , Böttcher Chotima , Brockmann Leonie , Burns Marie , Busch Dirk H. , Cameron Garth , Cammarata Ilenia , Cassotta Antonino , Chang Yinshui , Chirdo Fernando Gabriel , Christakou Eleni , Čičin‐Šain Luka , Cook Laura , Corbett Alexandra J. , Cornelis Rebecca , Cosmi Lorenzo , Davey Martin S. , De Biasi Sara , De Simone Gabriele , del Zotto Genny , Delacher Michael , Di Rosa Francesca , Di Santo James , Diefenbach Andreas , Dong Jun , Dörner Thomas , Dress Regine J. , Dutertre Charles‐Antoine , Eckle Sidonia B. G. , Eede Pascale , Evrard Maximilien , Falk Christine S. , Feuerer Markus , Fillatreau Simon , Fiz‐Lopez Aida , Follo Marie , Foulds Gemma A. , Fröbel Julia , Gagliani Nicola , Galletti Giovanni , Gangaev Anastasia , Garbi Natalio , Garrote José Antonio , Geginat Jens , Gherardin Nicholas A. , Gibellini Lara , Ginhoux Florent , Godfrey Dale I. , Gruarin Paola , Haftmann Claudia , Hansmann Leo , Harpur Christopher M. , Hayday Adrian C. , Heine Guido , Hernández Daniela Carolina , Herrmann Martin , Hoelsken Oliver , Huang Qing , Huber Samuel , Huber Johanna E. , Huehn Jochen , Hundemer Michael , Hwang William Y. K. , Iannacone Matteo , Ivison Sabine M. , Jäck Hans‐Martin , Jani Peter K. , Keller Baerbel , Kessler Nina , Ketelaars Steven , Knop Laura , Knopf Jasmin , Koay Hui‐Fern , Kobow Katja , Kriegsmann Katharina , Kristyanto H. , Krueger Andreas , Kuehne Jenny F. , Kunze‐Schumacher Heike , Kvistborg Pia , Kwok Immanuel , Latorre Daniela , Lenz Daniel , Levings Megan K. , Lino Andreia C. , Liotta Francesco , Long Heather M. , Lugli Enrico , MacDonald Katherine N. , Maggi Laura , Maini Mala K. , Mair Florian , Manta Calin , Manz Rudolf Armin , Mashreghi Mir‐Farzin , Mazzoni Alessio , McCluskey James , Mei Henrik E. , Melchers Fritz , Melzer Susanne , Mielenz Dirk , Monin Leticia , Moretta Lorenzo , Multhoff Gabriele , Muñoz Luis Enrique , Muñoz‐Ruiz Miguel , Muscate Franziska , Natalini Ambra , Neumann Katrin , Ng Lai Guan , Niedobitek Antonia , Niemz Jana , Almeida Larissa Nogueira , Notarbartolo Samuele , Ostendorf Lennard , Pallett Laura J. , Patel Amit A. , Percin Gulce Itir , Peruzzi Giovanna , Pinti Marcello , Pockley A. Graham , Pracht Katharina , Prinz Immo , Pujol‐Autonell Irma , Pulvirenti Nadia , Quatrini Linda , Quinn Kylie M. , Radbruch Helena , Rhys Hefin , Rodrigo Maria B. , Romagnani Chiara , Saggau Carina , Sakaguchi Shimon , Sallusto Federica , Sanderink Lieke , Sandrock Inga , Schauer Christine , Scheffold Alexander , Scherer Hans U. , Schiemann Matthias , Schildberg Frank A. , Schober Kilian , Schoen Janina , Schuh Wolfgang , Schüler Thomas , Schulz Axel R. , Schulz Sebastian , Schulze Julia , Simonetti Sonia , Singh Jeeshan , Sitnik Katarzyna M. , Stark Regina , Starossom Sarah , Stehle Christina , Szelinski Franziska , Tan Leonard , Tarnok Attila , Tornack Julia , Tree Timothy I. M. , van Beek Jasper J. P. , van de Veen Willem , van Gisbergen Klaas , Vasco Chiara , Verheyden Nikita A. , von Borstel Anouk , Ward‐Hartstonge Kirsten A. , Warnatz Klaus , Waskow Claudia , Wiedemann Annika , Wilharm Anneke , Wing James , Wirz Oliver , Wittner Jens , Yang Jennie H. M. , Yang Juhao 
Published: December 2021

AbstractThe third edition of Flow Cytometry Guidelines provides the key aspects to consider when performing flow cytometry experiments and includes comprehensive sections describing phenotypes and functional assays of all major human and murine immune cell subsets. Notably, the Guidelines contain helpful tables highlighting phenotypes and key differences between human and murine cells. Another useful feature of this edition is the flow cytometry analysis of clinical samples with examples of flow cytometry applications in the context of autoimmune diseases, cancers as well as acute and chronic infectious diseases. Furthermore, there are sections detailing tips, tricks and pitfalls to avoid. All sections are written and peer‐reviewed by leading flow cytometry experts and immunologists, making this edition an essential and state‐of‐the‐art handbook for basic and clinical researchers.