bluuebunny/crossref_metadata_2025_split huggingface dataset:
Setd4 controlled quiescent c-Kit+ cells contribute to cardiac neovascularization of capillaries beyond activation
Authors: Xing Sheng , Tian Jin-Ze , Yang Shu-Hua , Huang Xue-Ting , Ding Yan-Fu , Lu Qian-Yun , Yang Jin-Shu , Yang Wei-Jun 
Published: June 2021

AbstractBlood vessels in the adult mammal exist in a highly organized and stable state. In the ischemic heart, limited expansion capacity of the myocardial vascular bed cannot satisfy demands for oxygen supply and the myocardium eventually undergoes irreversible damage. The predominant contribution of endogenous c-Kit+ cells is understood to be in the development and homeostasis of cardiac endothelial cells, which suggests potential for their targeting in treatments for cardiac ischemic injury. Quiescent cells in other tissues are known to contribute to the long-term maintenance of a cell pool, preserve proliferation capacity and, upon activation, facilitate tissue homeostasis and regeneration in response to tissue injury. Here, we present evidence of a Setd4-expressing quiescent c-Kit+ cell population in the adult mouse heart originating from embryonic stages. Conditional knock-out of Setd4 in c-Kit-CreERT2;Setd4f/f;Rosa26TdTomato mice induced an increase in vascular endothelial cells of capillaries in both neonatal and adult mice. We show that Setd4 regulates quiescence of c-Kit+ cells by the PI3K-Akt-mTOR signaling pathway via H4K20me3 catalysis. In myocardial infarction injured mice, Setd4 knock-out resulted in attenuated cardiomyocyte apoptosis, decreased infarction size and improved cardiac function. Lineage tracing in Setd4-Cre;Rosa26mT/mG mice showed that Setd4+ cells contribute to each cardiac lineage. Overall, Setd4 epigenetically controls c-Kit+ cell quiescence in the adult heart by facilitating heterochromatin formation via H4K20me3. Beyond activation, endogenous quiescent c-Kit+ cells were able to improve cardiac function in myocardial infarction injured mice via the neovascularization of capillaries.
Association of the transthyretin variant V122I with polyneuropathy among individuals of African ancestry
Authors: Parker Margaret M. , Damrauer Scott M. , Tcheandjieu Catherine , Erbe David , Aldinc Emre , Hawkins Philip N. , Gillmore Julian D. , Hull Leland E. , Lynch Julie A. , Joseph Jacob , Ticau Simina , Flynn-Carroll Alexander O. , Deaton Aimee M. , Ward Lucas D. , Assimes Themistocles L. , Tsao Philip S. , Chang Kyong-Mi , Rader Daniel J. , Fitzgerald Kevin , Vaishnaw Akshay K. , Hinkle Gregory , Nioi Paul 
Published: June 2021

AbstractHereditary transthyretin-mediated (hATTR) amyloidosis is an underdiagnosed, progressively debilitating disease caused by mutations in the transthyretin (TTR) gene. V122I, a common pathogenic TTR mutation, is found in 3–4% of individuals of African ancestry in the United States and has been associated with cardiomyopathy and heart failure. To better understand the phenotypic consequences of carrying V122I, we conducted a phenome-wide association study scanning 427 ICD diagnosis codes in UK Biobank participants of African ancestry (n = 6062). Significant associations were tested for replication in the Penn Medicine Biobank (n = 5737) and the Million Veteran Program (n = 82,382). V122I was significantly associated with polyneuropathy in the UK Biobank (odds ratio [OR] = 6.4, 95% confidence interval [CI] 2.6–15.6, p = 4.2 × 10−5), which was replicated in the Penn Medicine Biobank (OR = 1.6, 95% CI 1.2–2.4, p = 6.0 × 10–3) and Million Veteran Program (OR = 1.5, 95% CI 1.2–1.8, p = 1.8 × 10−4). Polyneuropathy prevalence among V122I carriers was 2.1%, 9.0%, and 4.8% in the UK Biobank, Penn Medicine Biobank, and Million Veteran Program, respectively. The cumulative incidence of common hATTR amyloidosis manifestations (carpal tunnel syndrome, polyneuropathy, cardiomyopathy, heart failure) was significantly enriched in V122I carriers compared with non-carriers (HR = 2.8, 95% CI 1.7–4.5, p = 2.6 × 10−5) in the UK Biobank, with 37.4% of V122I carriers having at least one of these manifestations by age 75. Our findings show that V122I carriers are at increased risk of polyneuropathy. These results also emphasize the underdiagnosis of disease in V122I carriers with a significant proportion of subjects showing phenotypic changes consistent with hATTR amyloidosis. Greater understanding of the manifestations associated with V122I is critical for earlier diagnosis and treatment.
Distinction between electron states formed at topological insulator interfaces with the trivial phase and vacuum
Authors: Kazakov A. S. , Galeeva A. V. , Artamkin A. I. , Ikonnikov A. V. , Ryabova L. I. , Dvoretsky S. A. , Mikhailov N. N. , Bannikov M. I. , Danilov S. N. , Khokhlov D. R. 
Published: June 2021

AbstractIn this paper, we show that electron states formed in topological insulators at the interfaces topological phase–trivial phase and topological phase–vacuum may possess different properties. This is demonstrated on an example of heterostructures based on thick topological Hg1−xCdxTe films, in which the PT-symmetric terahertz photoconductivity is observed. It is shown that the effect originates from features of the interface topological film–trivial buffer/cap layer. The PT-symmetric terahertz photoconductivity is not provided by electron states formed at the interface topological film–vacuum.
Confirming putative variants at ≤ 5% allele frequency using allele enrichment and Sanger sequencing
Authors: Yan Yan Helen , Chen Sherry X. , Cheng Lauren Y. , Rodriguez Alyssa Y. , Tang Rui , Cabrera Karina , Zhang David Yu 
Published: June 2021

AbstractWhole exome sequencing (WES) is used to identify mutations in a patient’s tumor DNA that are predictive of tumor behavior, including the likelihood of response or resistance to cancer therapy. WES has a mutation limit of detection (LoD) at variant allele frequencies (VAF) of 5%. Putative mutations called at ≤ 5% VAF are frequently due to sequencing errors, therefore reporting these subclonal mutations incurs risk of significant false positives. Here we performed ~ 1000 × WES on fresh-frozen and formalin-fixed paraffin-embedded (FFPE) tissue biopsy samples from a non-small cell lung cancer patient, and identified 226 putative mutations at between 0.5 and 5% VAF. Each variant was then tested using NuProbe NGSure, to confirm the original WES calls. NGSure utilizes Blocker Displacement Amplification to first enrich the allelic fraction of the mutation and then uses Sanger sequencing to determine mutation identity. Results showed that 52% of the 226 (117) putative variants were disconfirmed, among which 2% (5) putative variants were found to be misidentified in WES. In the 66 cancer-related variants, the disconfirmed rate was 82% (54/66). This data demonstrates Blocker Displacement Amplification allelic enrichment coupled with Sanger sequencing can be used to confirm putative mutations ≤ 5% VAF. By implementing this method, next-generation sequencing can reliably report low-level variants at a high sensitivity, without the cost of high sequencing depth.
Nano-FTIR spectroscopic identification of prebiotic carbonyl compounds in Dominion Range 08006 carbonaceous chondrite
Authors: Yesiltas Mehmet , Glotch Timothy D. , Sava Bogdan 
Published: June 2021

AbstractMeteorites contain organic matter that may have contributed to the origin of life on Earth. Carbonyl compounds such as aldehydes and carboxylic acids, which occur in meteorites, may be precursors of biologically necessary organic materials in the solar system. Therefore, such organic matter is of astrobiological importance and their detection and characterization can contribute to the understanding of the early solar system as well as the origin of life. Most organic matter is typically sub-micrometer in size, and organic nanoglobules are even smaller (50–300 nm). Novel analytical techniques with nanoscale spatial resolution are required to detect and characterize organic matter within extraterrestrial materials. Most techniques require powdered samples, consume the material, and lose petrographic context of organics. Here, we report the detection of nanoglobular aldehyde and carboxylic acids in a highly primitive carbonaceous chondrite (DOM 08006) with ~ 20 nm spatial resolution using nano-FTIR spectroscopy. Such organic matter is found within the matrix of DOM 08006 and is typically 50–300 nm in size. We also show petrographic context and nanoscale morphologic/topographic features of the organic matter. Our results indicate that prebiotic carbonyl nanoglobules can form in a less aqueous and relatively elevated temperature-environment (220–230 °C) in a carbonaceous parent body.
A 16-year prospective cohort study to evaluate effects of long-term fluctuations in obesity indices of prediabetics on the incidence of future diabetes
Authors: Safari Shahla , Abdoli Maryam , Amini Masoud , Aminorroaya Ashraf , Feizi Awat 
Published: June 2021

AbstractThis study aimed to evaluate the patterns of changes in obesity indices over time in prediabetic subjects and to classify these subjects as either having a low, moderate, and high risk for developing diabetes in the future. This study was conducted among 1228 prediabetics. The patterns of changes in obesity indices based on three measurements including first, mean values during the follow-up period, and last visit from these indices were evaluated by using the latent Markov model (LMM). The mean (standard deviation) age of subjects was 44.0 (6.8) years and 73.6% of them were female. LMM identified three latent states of subjects in terms of change in all anthropometric indices: a low, moderate, and high tendency to progress diabetes with the state sizes (29%, 45%, and 26%), respectively. LMM showed that the probability of transitioning from a low to a moderate tendency to progress diabetes was higher than the other transition probabilities. Based on a long-term evaluation of patterns of changes in obesity indices, our results reemphasized the values of all five obesity indices in clinical settings for identifying high-risk prediabetic subjects for developing diabetes in future and the need for more effective obesity prevention strategies.
Saturated adaptive barrier sliding mode control with state‐dependent uncertainty limit
Authors: Ma Haifeng , Liu Weixiang , Li Yangmin , Liu Zhanqiang , Tian Sukun 
Published: June 2021

AbstractThis paper proposed a saturated adaptive barrier sliding mode control (SABSMC) for disturbed systems with parameter uncertainty, external disturbance, and input saturation. One distinctive feature of the proposed method is that even under the influence of input saturation, it is able to drive the system trajectory into a prespecified neighbourhood of the sliding surface, independent of the upper limit of lumped disturbance. Moreover, attributing to the designed adaptive laws, the switching gain of the proposed method can be adaptively adjusted in a wise manner. Another attractive feature of the proposed method is that it removes any presumption of constant upper limit on the lumped disturbance. Instead, SABSMC utilises a more rational state‐dependent upper limit assumption. The system dynamics of the closed‐loop system is analysed via the Lyapunov method in the presence of parameter uncertainty, external disturbance, and input saturation. The effectiveness of the proposed method is confirmed through numerical simulations.
Effects of aviation kerosene contents on the environment and method of its cleansing
Authors: Isokov Yusuf , Yodgorov Normaxmat , Sultonov Sadulla , Khalilov Sanjar 
Published: June 2021

The high content of sulfur compounds in aviation kerosene produced at the Bukhara Oil Refinery (BOR) is causing great damage to the environment. Purification of sulfur compounds in aviation kerosene using activated charcoal is effective. One way to remove sulfur compounds from aviation kerosene is to clean them using activated charcoal (adsorbent) obtained based on fruit pods.
Purification of aviation kerosene from mercaptans is a chemical adsorption process, using activated charcoal obtained based on shells of fruit seeds (walnuts, peaches, apricots, etc.). The adsorption of activated carbon obtained on the basis of husks of fruit seeds (walnuts, peaches, apricots, etc.) depends on the physicochemical properties; such activated charcoals were obtained by thermochemical methods.
Biological treatment of industrial and domestic wastewater of a brewery in Uzbekistan
Authors: Egamberdiev N.B. , Sharipjonova Zilola , Nasibov Bobur , Khomidov A.O. , Alimova M.I. , Abdumalikov A.A. 
Published: June 2021

During the period of water shortage in the Republic, modern resource-saving irrigation methods and the use of purified and industrial waters and their reuse in irrigation is an urgent problem in ecology. Among the methods for treating industrial wastewater in a cheaper, cost effective way is the biological treatment method. It is the study of the effectiveness of biological treatment of industrial wastewater from primary winemaking using selected strains of aquatic plants (pistia).
The object of wastewater research is selecting a Pistia algae strain, carrying out biochemical, hydrochemical analyses of wastewater before and after treatment, and the chemical composition of the Pistia algae biomass. All studies were carried out according to the standard studies of UzGOST for waste and drinking water and algological methods used by the Institute of Botany of ANRUz, State Enterprise "Institute GIDROINGEO", etc.
The efficiency of biological purification of wastewaters of primary winemaking by higher aquatic plants of the pistia was established. With the help of the research carried out, the wastewater treatment of the food plant, in particular, the Kibray wine station with the Pistia algae, was established:

the optimal parameters of growth, development and purification capacity of pistia algae were established for various variants of experiments and wastewater samples;
designed and assembled a semi-industrial plant for biological wastewater treatment of the Kibray wine station and carried out work on industrial wastewater treatment.

Wastewater from the Kibray wine station contains organic compounds, namely yeast sediments, proteins, fats, carbohydrates, fiber, which are food for Pistia algae. Pistia biomass obtained after cultivation in wastewater after sterilization can be used as feed in livestock and poultry farming, as it contains a large number of proteins, fats and carbohydrates.
Influencing of land transport load on structure of backfill arched Road Bridge
Authors: Miralimov Mirzakhid , Ishankhodjaev Abdurakhman , Almenov Khudaybergan , Muminov Eldior 
Published: June 2021

In this paper, for the objective assessment of stress-strain state parameters of backfill arched road bridge, the research work is carried out, where the model of reinforced concrete, corrugated structure is exposed to modern land transport load.
In the course of numerical studies, stresses and deformations in sections of structure and the soil are determined. These studies allow revealing the mechanism of operation of corrugated reinforced concrete structures in the body of an embankment of highways when passing modern transport loads along with them.
Compressive strength properties of hyper-compacted concrete
Authors: Khasanov Bakhridin , Tillaev Alisher , Mirzaev Timur 
Published: June 2021

The most important property of a concrete mix is concrete workability, i.e., the ability of the mixture to spread and take a given form while maintaining solidity and uniformity.The main influence on the workability of the concrete mixture is exerted by water consumption and, in part, cement consumption. Workability is determined by the mobility of the concrete mixture at the time of filling the mold and plasticity, i.e., the ability to deform without breaking the continuity.In the process of vibrating and pressing the concrete mixture placed in the mold, the total volume of the mixture changes until the pressure is balanced by the resistance forces. Deformation of concrete mix or, more precisely, freshly laid concrete with any compaction methods, including vibration compaction, is divided into elastic (reversible) and residual (irreversible). Residual deformations during vibration compaction occur as a result of water squeezing out and redistribution of aggregate fractions.Permanent deformation is part of the total. Its value at the same composition of the concrete mixture depends on the shape and size of the pressed sample. At the same time, it is noteworthy that after reaching a certain pressure, only elastic deformations will be characteristic of the freshly laid concrete mixture. So, A.D. Nikitin, in the course of the experiments, found that at a pressure of 2.2 MPa, the elastic moduli of the components of the concrete mixture have the following values: for cement paste - 0.16 · 104 MPa, aggregate - 4.5 · 104 MPa and air - 3 MPa ... After reaching a static pressure of 2.2 MPa, the compressible mixture showed only elastic deformation. This indicates that by the time the specified pressure was reached, the relative movement of the aggregates had ended, i.e., they are located most compactly.
Development of high-strength concrete with the addition of a universal superplasticizer
Authors: Barkhanadzhyan Aida , Riskulov Alimjon , Karimov Maksud , Khakimov Ravshan , Ibragimov Botir 
Published: June 2021

Automobile roads perform a particularly important, responsible function in the movement of every automobile, transport equipment. The quality of automobile roads determines the reliability of road construction and automotive equipment, the resource of their work, and, most importantly, ensuring traffic safety. However, the construction of roads and construction methods with the expiration of a certain time undergo significant changes associated with the intensive development of road transport, road construction equipment, an increase in the intensity and flow of traffic. In this regard, there is a need to build high-quality roads and cover roads with durable concrete, which can be achieved by upgrading with composite chemical additives in the composition of concrete - a modernized superplasticizer. The object of this work is a superplasticizer for producing wear-resistant concrete. The introduction of a superplasticizer into the composition of concrete makes it possible to obtain especially strong concrete with an increased service life, which in turn will significantly reduce material costs for raw materials, repair work, and labor resources. The purpose of this study is to obtain a highly effective superplasticizer and particularly durable wear-resistant concrete for road surfaces. To achieve this goal, it is necessary to solve the following tasks:

prepare raw materials, develop an optimal component composition and obtain a superplasticizer;
in laboratory conditions to obtain a prototype of concrete;
determine the quality indicators of the resulting concrete by GOST;
to conduct pilot tests for implementation in production.
Conditions for bending cotton stalks of a combined aggregation bender and their implementation
Authors: Khudayarov Berdirasul , Kuziyev Ulugbek , Sarimsakov Bakhtiyar 
Published: June 2021

As the object of the research, the cotton stalk and its physical and mechanical properties, the elastic of the combined aggregate and the spherical disk were accepted. Like many other European countries, our country has also developed minimal, resource-saving and zero technologies for the cultivation of agricultural products, and their technical solutions are being supported and implemented by farmers and clusters. In particular, in the preparation of cotton fields for sowing for the next year in one pass of machinery in cotton growing, the research was conducted on the development of a minimum technology and a combined unit that implements it. In the implementation of this technology, the cotton stalks are tilted to the side furrow in the direction of movement of the aggregate, and the root is laid on the bottom of the furrow with the soil. The bottom layer of the existing pile is then divided into two parts, each of which is rolled over the cotton stalks on the side edges on both sides, where a new pile is formed. Once the aggregate has passed, the existing ridges are replaced by ridges with cotton stalks buried under them, and ridges are formed in place of the existing ridges.
In the study, the angle of the axis of symmetry was determined by the angle of the stalks, and in the laboratory, the amount of force required to bend the stalks was determined using a specially prepared stand.
In the study, the width of the flexible cover is 45 cm, the mounting angle of the straight part of the flexible relative to the direction of movement is 35-450, the radius of curvature should be 100 mm, the mounting height relative to the ridge should be 15 cm, and the shape was determined.
Bending of cotton stalks standing on the ridge in the direction of aggregate movement to the side ridge, digging them with root soil, longitudinal laying at the bottom of this ridge can be done using a spherical disk.
Method for assessing the energy efficiency of a vehicle taking into account the load under operating conditions
Authors: Abdurazzokov Umidulla , Sattivaldiev Bakhramjan , Khikmatov Ravshan , Ziyaeva Shakhnoza 
Published: June 2021

In operation conditions, the transport work of a vehicle is estimated by the increment in the mass of the freight over the distance traveled. This criterion does not characterize the mechanical work of the vehicle in the transport process. Without analyzing the energy costs of performing mechanical work, it is impossible to assess the energy efficiency of a vehicle.
The energy efficiency of a vehicle is defined as the ratio of the mechanical work performed by the vehicle to the potential energy of the source. In this paper, it is proposed to determine the engine torque by fuel consumption. The engine torque value depends on the energy required for driving the vehicle.
Based on the analysis of the results of computational and experimental studies, a method for assessing the energy efficiency of a vehicle with an internal combustion engine is proposed. The reliability of the results obtained is substantiated by the test results and the available information in practice.
Specialized railway carriage for grain
Authors: Ruzmetov Yadgor , Valieva Dilmira 
Published: June 2021

This article deals with the worked out specialized railway carriage for grain for the transportation process throughout the Republic of Uzbekistan railway network, with the unconditional provision of train traffic safety, which is one of the most important components of the rolling stock of railway transport.
The features of the load-bearing structure of the railway carriage for grain are that it is made based on a four-axle platform and a universal container. Evaluation of the strength of the metal structure of the grain car was carried out following the requirements of the “Standards for the calculation and design of new and modernized cars of the railways of the Ministry of Railways with a gauge of 1520 mm (non-self-propelled)”.
Based on theoretical studies, it was justified that the design of a railway carriage for grain with a capacity of 83.6 m3 and a carrying capacity of 65 tons. For the first test of a railway carriage for grain, the number 43925684 was assigned.
The proposed new grain carriage will reduce operating costs and reduce rolling stock downtime during reloading operations. It will create the possibility of fulfilling contractual agreements drawn up for small volumes of traffic within the country.