bluuebunny/crossref_metadata_2025_split huggingface dataset:
Tao-Hong-Si-Wu Decoction improves depressive symptoms in model rats via restorations of neural transmitters and ameliorations of BDNF-CREB-arginase I axis disorders
Authors: Zhang Xiaoping , Li Zeng , Shen Chuanpu , He Jinzhi , Wang Longfei , Di Lei , Rui Bin , Li Ning , Zhicheng Liu 
Published: October 2021

Abstract
Background

Depressive disorder (DD) has become a global health problem. Applications of Chinese medicine have been demonstrated to be potential in the treatment of DD. Tao-Hong-Si-Wu decoction (TSD), a traditional Chinese medicine formula is widely used to treat ameliorate anemia, liver and heart dysfunctions, and inflammation. Such symptoms are also associated with DD. Hence, our initial hypothesis was to observe and explore the targets of the effect of TSD on DD.
Methods

The DD model was established by chronic unpredictable mild stress (CUMS) in rats. The measurements of body weight and behavioral tests were performed to confirm the success of modeling and observe the effect of TSD on the model animals. A gas-chromatography coupled with mass spectrometry (GC-MS)-based metabolomic analysis was conducted to reveal the metabolic characteristics related to the curative effect of TSD. Serum serotonin and arginase I (Arg I), which are associated with the key feature metabolites responsible for the effect of TSD on depression, were assessed by an ELISA assay. Proteins in the Brain-derived neurotrophic factor (BDNF)/ Tropomyosin receptor kinase B (TrkB)/cAMP response element-binding protein (CREB) signaling, which lead to the regulations of Arg I, were analyzed using Western blot assay.
Results

Body weight losses and increased immobility durations in the behavioral tests were observed in the CUMS rats while such features were attenuated in the TSD-treated rats. General decreases in serum amino acids and energetic metabolites were found in CUMS rats. Significant increased ornithine and urea, the products of arginase degradation, were also characterized in the DD-mimicked rats. These metabolic dysregulations were reversed in the TSD-treated groups. Upregulated Arg I and downregulated serotonin were observed in the model group but the dysregulations were improved in the TSD-treated groups. Compared with the blank control, lower expressions of BDNF, TrkB, extracellular signal-regulated kinases (ERK), p-ERK, and CREB were found in the hippocampus of CUMS rats. Expressions of the proteins were restored in all the TSD-treated groups.
Conclusions

TSD improves depression-like symptoms in CUMS rats, which may be primarily related to its effect both on enhancing the release of neural transmitters such as glycine and serotonin and on the improvement of disorders of the BNDF-CREB-Arg I axis.
Effects of extreme water levels on water quality in a large shallow eutrophic lake (Changhu Lake, China)
Authors: Wang Haiyan , Yang Jun , Li Tao , Zhu Jianqiang , Liu Zhangyong 
Published: October 2021

Abstract
Changhu Lake, a large shallow eutrophic lake in central China, experienced an extreme low water level event from November 2015 to January 2016 followed by an extreme high water level event in July 2016. In this study, we examined the effects of two extreme water levels on the nutrient status of Changhu Lake over five years. The nutrient concentrations in Changhu Lake showed significant interannual variations and the water quality of sites in the western part of Changhu Lake was better compared to sites at the outlet of the lake. In late 2015, the effect of low water levels led to a significant increase in nutrient concentrations. After July 2016, however, the high water level occurred leading to a marked decrease in nutrient concentrations. These changes in nutrient parameters were strongly related to the water level fluctuations. The dilution effect was the key process that determined the variations of nutrient parameters in Changhu Lake. As extreme water levels are likely to become more frequent during the twenty-first century, this work may provide some insights into the conservation and management of lake ecosystems in the face of climate change and human activity.
Scaling Up Tobacco Cessation within TB Programmes: Findings from a Multi-Country, Mixed-Methods, Implementation Study.
Authors: Elsey Helen , Azdi Zunayed Al , Regmi Shophika , Baral Sushil , Fatima Razia , Feroze Fariza , Huque Rumana , Karki Jiban , Khan Dost Mohammad , Khan Amina , Khan Zohaib , Li Jinshuo , Noor Maryam , Arjyal Abriti , Shrestha Prabin , Ullah Safat , Siddiqi Kamran 
Published: October 2021

Abstract
Background: Brief behavioural support can effectively help TB patients to quit smoking and improve their outcomes. In collaboration with TB programmes in Bangladesh, Nepal and Pakistan, we evaluated implementation and scale-up of cessation support using four strategies: i) brief tobacco cessation intervention ii) integration of tobacco cessation within routine training iii) inclusion of tobacco indicators in routine records and iv) embedding research within TB programmes.Methods: We used mixed methods of observation, interviews and routine data within WHO’s ExpandNet framework for scale-up. We aimed to understand the extent of, and strategies which facilitated vertical scale-up (institutionalisation) within 59 health facility learning sites in Pakistan, 18 in Nepal and 15 in Bangladesh and horizontal scale-up (increased coverage beyond learning sites). We observed training and surveyed 169 TB health workers to measure changes in their confidence to deliver cessation. Routine TB data from the learning sites was analysed to assess delivery of the intervention and use of TB forms revised to report smoking status and cessation support provided. A purposive sample of TB health workers, managers and policymakers were interviewed (Bangladesh: n=12; Nepal n=13; Pakistan n=19;). Costs of scale-up were estimated using activity-based cost-analysis. Results: Routine data indicated health workers in learning sites asked all TB patients about tobacco use and offered them cessation support. Qualitative data showed use of intervention materials, often with adaptation and partial implementation in busy clinics. Short (1-2 hours) training integrated within existing programmes increased mean confidence to deliver cessation by 17% (95% CI: 14% to 20%). A focus on health system changes (reporting, training, supervision) facilitated vertical scale-up. Dissemination of materials beyond learning sites and changes to national reporting forms and training indicated horizontal scale-up. Embedding research within TB health systems was crucial for horizontal scale-up and required dynamic use of tactics e.g. alliance-building, engagement in wider policy process, use of insider-researchers, and deep understanding of health system actors and processes. Conclusions: System-level changes within TB programmes may enable routine delivery of cessation support to TB patients. These strategies are inexpensive and, with concerted efforts from TB programmes and donors, tobacco cessation can be institutionalised at-scale.
Study on the effects of Polyphyllin I and Curcumin on liver cancer based on the cross-action of ferroptosis and energy metabolism
Authors: Yu Shuxian , Gao Wenhui , Zeng Puhua , Lu Mingxuan , Tan Xiaoning , Zhang Zhen , Liu Zhuo , Hou Zongwei , Liu Jiyong 
Published: October 2021

Abstract
Background: To investigate the effect and mechanism of Polyphyllin I(PPI) and Curcumin(Cur) on human liver cancer HepG2 and HepG2.2.15 cells. Methods: Download the hepatocellular carcinoma specimens and normal control specimens from the TCGA database, take the intersection with ferroptosis-related genes, and screen the differentially expressed ferroptosis genes; again, make the intersection with the selected differential genes related to energy metabolism; conduct survival analysis; construct prognosis Risk scoring model and evaluation of model performance; through molecular docking to verify the binding effect of PPI, Cur and Ribonucleoside-diphosphate reductase subunit M2(RRM2), SRC(SRC), Acetyl-CoA carboxylase alpha(ACACA) and other genes. Human hepatocellular carcinoma cells were cultured in vitro, PPI and Cur intervened, and Cell Counting Kit-8(CCK-8) was used to detect cell inhibition rate; FeRhoNox-1 fluorescent probe staining to observe the intracellular Fe 2+ status; lactate dehydrogenase (LDH) release Experiment to detect cell LDH release rate; JC-1 staining to detect mitochondrial membrane potential; reactive oxygen species(ROS) kit to detect ROS level;Western blotting (WB) to detect RRM2 and SRC , ACACA and other genes protein expression levels. Results: Through screening, 25 differential genes related to ferroptosis and energy metabolism in liver cancer were obtained;; through survival analysis, three ferroptosis-related genes, such as RRM2, SRC, and ACACA, were obtained，the results showed that these three genes showed high expression and predicted poor overall survival(OS) and disease-free survival(DFS); molecular docking results showed that PPI, Cur It has good affinity with RRM2, SRC and ACACA. The results of in vitro experiments show that PPI and Cur inhibit cell proliferation in a concentration-dependent manner ( P <0.01). PPI and Cur can significantly increase the intracellular Fe2+ concentration, LDH release rate and intracellular ROS levels of HepG2, HepG2.2.15 ( P <0.01), and the effect on mitochondrial membrane potential was significantly lower than that of the blank group ( P <0.01), and significantly down-regulated the protein expression levels of RRM2, SRC, and ACACA ( P <0.01). Conclusion: The high expression of RRM2, SRC, ACACA and other three genes related to ferroptosis and energy metabolism in liver cancer indicate poor OS and DFS; PPI and Cur can up-regulate the LDH release rate, ROS and Fe 2+ levels of liver cancer cells, and down-regulate the cell mitochondrial membrane potential and other methods to inhibit the proliferation of liver cancer cells; and down-regulate the expression of RRM2, SRC, ACACA and other proteins to affect the prognosis of liver cancer.
A Novel Fuzzy Approach to Gas Pipeline Risk Assessment Under Influence of Ground Movement
Authors: Malinowska Agnieszka , Cui Ximin , Salmi Ebrahim Fathi , Hejmanowski Ryszard 
Published: October 2021

Abstract
The gas transport infrastructure is frequently localized in areas subjected to anthropogenic movements and strains. The potential impact of such deformations on the gas pipeline in the aspect of its damaging can be properly assessed by, e.g. by predicting strains, taking into account the causes of terrain movement. On the other hand the hazard is also related to technological factors like design of the pipeline. The presented method is based on artificial intelligence methods allowing for evaluation of probability of failure risk in gas supply pipeline sections. The Mamdani fuzzy inference was used in the study. Uncertainty of variables characterizing the resistance of the gas pipeline and predicted continuous deformations of ground surface were accounted for in the model by using triangular-shaped membership functions. Based on the surface deformations and gas pipeline resistance and the inference model one can make prediction when the gas pipeline is hazarded. The proposed model can contribute to the protection, cost optimization of the designed pipelines and to the repairs of the existing gas pipelinesTrial registration number and date of registration: COAL-D-21-00255, 27.09.2021
Evaluation of Neonatal Cardiopulmonary and Anthropometric Indices: A Key Predictive Clinical Biomarker for Neonatal Health Status
Authors: Olowe Gideon T. , Ovuakporaye Simon I , Nwogueze Bartholomew C. , Oyovwi Mega O. , Odokuma Emmanuel I. , Emojevwe Victor 
Published: October 2021

Abstract
Background

Neonatal mortality has been reported as a major societal health menace across the globe. Hence, this study, which adopted a hospital-based cross-sectional type of experimental research design, aimed at examining the clinical indication of some selected cardiopulmonary and anthropometric indices as key predictive clinical biomarkers for neonatal survival. The subjects were divided into three groups; experimental Group 1 consisted of 80 pre-term neonates within 28 weeks; experimental Group 2 consisted of 80 pre-term neonates within 33 weeks; and the control group, which consisted of 80 apparently healthy, term neonates. For each subject selected, cardio-pulmonary parameters and anthropometric variables were obtained daily and compared. ANOVA, correlation coefficients, and linear regression were used to compare statistical measures. A discrimination accuracy test of previous records using cut points available from previous models was conducted for ease of estimating the possibility of neonatal survival from assayed variables.
Results

Following data collection, sorting, and analysis, the study found a statistically significant decrease (p < 0.05) in average systolic blood pressure (SBP) values of preterm neonates when compared with term neonates. This, however, was the reverse for DBP, which was proved to have increased in preterm neonates compared to term neonates. The study also observed a statistically significant decrease (p < 0.05) in RR and OSL levels of preterm neonates compared to term neonates.
Conclusion

In conclusion, cardiopulmonary and anthropometric status could be useful predictive biomarkers in clinical trials to provide insight into the extent of compromise in newborn health. This will help to minimize the death of neonates by ensuring a better treatment protocol/regimen is given. A corroborative study with an event-driven approach that assays more molecular or other non-physiological variables is recommended.
Characterization of Bacterial Community of Rumen in Dairy Cows With Laminitis
Authors: Gao Jian , Liu Bo , Li Shuang , Mu Ruiying , Zhang Naisheng , Hu Xiaoyu , Fu Yunhe 
Published: October 2021

Abstract
Background: Laminitis- an inflammation of lamella, could cause great economic loss to dairy industry, which has attracted wide attention around the world. In recent years, microbiota is considered as one of the vital parts that played significant role in various diseases processes. However, current studies are far from sufficient. Aim of this study is to explore the characteristics of ruminal microbiota in laminitis cows. Methods: The serum of bovines with or without laminitis was collected to detect concentrations of lipopolysaccharide (LPS), lactic acid, and histamine, and the ruminal fluid was collected for 16S rRNA sequence analysis. Results: The results showed that a significant increase in LPS and lactic acid levels in laminitis group comparing to control group cows. In addition, the higher abundance of bacteria that -riches acid-enhancing metabolites, namely, Candidatus Saccharimonas, Saccharofermentans, Erysipelotrichaceae UCG-009, Erysipelotrichaceae UCG-008, Clostridium papyrosolvens and Ruminococcaceae bacterium AE2021 were detected in the rumen fluid from laminitis bovines. Conclusions: This article confirmed that difference of rumen microbiota were occurred in rumen between health and laminitis bovines. The elevated abundance of bacteria that riches acid- enhancing metabolites, as well as increased the concentration of lactic acid and LPS could be harmful factors to bovines and increase risks of laminitis.
Characterization and Modeling Study on Softening and Seepage Behavior of Weakly Cemented Sandy Mudstone After Water Injection
Authors: Guo Junting , Teng Teng , Zhu Xiaoyan , Wang Yuming , Li Zhaolong 
Published: October 2021

Abstract
Water injection induced rock softening and the associated water seepage characteristics are the common and basic problems in hard roof pressure relief, underground reservoir construction and the prevention of mine water disaster. In this paper, a series of laboratory studies was carried out to investigate these characteristics with the weakly cemented sandy mudstone collected from Shendong Buertai coal mine, China. The characteristics of water softening and the stress-seepage interactions in saturated weekly cemented sandy mudstone were directly obtained. Then a modification method of the constitutive model for rock mass considering the softening effect and a stress-damage-driven model for permeability evolution were established. Research results show that water saturation reduces the tensile strength, compressive strength and cohesion by 56%, and reduces the elastic modulus by 28%. The hydraulic effect on Poisson’s ratio and internal friction angle is negligible. The relationship between the permeability of weakly cemented sandy mudstone with complete compaction deformation is to be divided into three stages of seepage shielding, seepage surge and seepage recovery. Rock permeability in each stage has a negative exponential relationship with the effective stress. This research provides a theoretical basis for the researches of hydro-mechanical couplings on weakly cemented sandy mudstone, which is insightful for rock engineering practice.
A Combined Proteomics and Metabolomics Profiling to Investigate the Genetic Heterogeneity of Autistic Children
Authors: Shen Liming , Zhang Huajie , Lin Jing , Gao Yan , Liu Xukun , Chen Margy , Khan Naseer Ullah , Tang Xiaoxiao , Hong Qi , Feng Chengyun , Zhao Yuxi , cao xueshan 
Published: October 2021

Abstract
Background: Autism spectrum disorder (ASD) has become one of the most common neurological developmental disorders in children. However, the study of ASD diagnostic markers faces significant challenges due to the existence of heterogeneity. Methods: In this study, genetic testing was performed on children who were clinically diagnosed with ASD. Children with ASD susceptibility genes and healthy controls were studied. The proteomics of plasma and peripheral blood mononuclear cells (PBMCs) as well as plasma metabolomics were carried out. Results: The results showed that although there was genetic heterogeneity in children with ASD, the differentially expressed proteins (DEPs) in plasma, PBMCs, and differential metabolites in plasma could still effectively distinguish autistic children from controls. The mechanism associated with them focus on several common and previously reported mechanisms of ASD. Limitations: The number of samples carrying risk genes in omics research is limited. In further research, a large sample size is required. A group of children who have been diagnosed with ASD but have not been detected to carry risk genes should also be included.Conclusion: The biomarkers for ASD diagnosis could be found by taking DEPs and differential metabolites into consideration. Integrating omics data, glycerophospholipid metabolism and N-glycan biosynthesis might play a critical role in the pathogenesis of ASD.
Analysis of the Psychometric Properties of the Sense of Coherence Scale (SOC-13) in Patients With Cardiovascular Risk Factors: A Study of the Method Effects Associated With Negatively Worded Items
Authors: Domínguez-Salas Sara , Andrés-Villas Montserrat , Sampol Aina Riera , Tauler Pedro , Bennasar-Veny Miquel , Aguilo Antoni , Rivera Francisco 
Published: October 2021

Abstract
Purpose: The objectives of this study were to analyze the psychometric properties of the Sense of Coherence Scale (SOC-13), determine the role of the method effect in the performance of the instrument, and identify the relationship with health perception, quality of life, and sleep quality in patients at cardiovascular risk. Methods: The final sample consisted of 293 patients at cardiovascular risk, with a mean age of 61.9 years (SD = 8.8), 49.8% of whom were women. The SOC-13, the Patient Health Questionnaire (PHQ-9) and the Mos Sleep Scale were administered. The participant´s self-perceived health and quality of live were also evaluated. All analyses were carried out with SPSS 26.0 and EQS 6.1 statistical software. Results: The results showed an adequate reliability for the SOC-13, with a Cronbach's alpha of .789. The fit of the structures was not adequate in any of the cases (one factor, three factors, one second-order factor, and three first-order factors). By adding a latent factor resulting from the method effect, the three structure models showed an improved fit. Moreover, positive correlations were found with health perception, perceived quality of life, and perceived sleep quality. Conclusion: In conclusion, it appears that the SOC-13 is a suitable instrument for patients with cardiovascular risk in Spain, and it is also an indicator of health perception, quality of life, and perceived quality of sleep. Control of the method effect improves the fit of the instrument’s structure.Trial registration: International Standard Randomized Controlled Trial Number: ISRCTN76069254, 08/04/2015 retrospectively registered.
Self-reported Symptom Burden Among Patients Attending Public Health Care Facilities in India: Looking through ICPC-3 Lens
Authors: Gupta Priti , Bharati Bhavna , Sahu Kirti Sundar , Mahapatra Pranab , Pati Sanghamitra 
Published: October 2021

Abstract

Objectives: The objectives of this study were: 1) to describe the socio demographics and classify chief complaints and reason to visit facilities of patients presenting to public healthcare facilities; 2) To explore difference in these complaints and ICPC-3 groups across socio-demographic and health system level.Design: Cross sectional studySettings: This study was conducted in three districts of Odisha, India. Within each district, the District hospital, one SDH (if available), two CHCs and two PHCs were selected. Thus a total of three DHHs, three SDHs, six CHCs and six PHCs were covered. Two tertiary health care facilities were also be included. Patients aged 18 years and above, attending the Outpatient Departments (OPD) of sampled health facilities were chosen as study participants through systematic random sampling. Results: In total 3044 patients were interviewed. In general, 65% of the sample reported symptoms as their chief complain for reason of encounter whereas 35% reported for disease and diagnosis. Most common reasons to encounter health facilities are fever, hypertension, abdominal pain, chest pain, arthritis, skin disease, cough, diabetes, and injury. Among symptoms categories, highest patients reported for general category (29%) followed by digestive system (16%). In the disease category circulatory system has the highest proportion followed by musculatory system. In symptom categories general, digestive and musculatory system were the key systems for reason of encounter in outpatient department irrespective of different groups of population. In terms of different tiers of health system, the top three reasons to visit OPD were dominated by circulatory system, respiratory system, musculatory system. Conclusion: This is the first Indian study using ICPC-3 classification for all the three levels of health care. Irrespective of age, socio economic variables and tiers of healthcare top three groups to visit public health facility according to ICPC-3 classification were consistent i.e., general, digestive, and circulatory. Implementation of standard management and referral guidelines for common diseases under these group will improve the quality and burden at public health facilities in India.
The Hopewell airburst event, 1699-1567 years ago (252-383 CE)
Authors: Tankersley Kenneth Barnett , Meyers Stephen D. , Meyers Stephanie A. , Jordan James A. , Herzner Louis , Lentz David L. , Zedaker Dylan 
Published: October 2021

Abstract
Meteorites, silicious vesicular melt glass, Fe and Si-rich magnetic spherules, positive Ir and Pt 25 anomalies, and burned charcoal-rich Hopewell habitation surfaces demonstrate that a cosmic airburst event occurred over the Ohio River valley during the late Holocene. A comet-shaped earthwork was constructed near the airburst epicenter. Twenty-nine radiocarbon ages demonstrate that the event occurred between 252 and 383 CE, a time when 69 near-Earth comets were documented. While Hopewell people survived the catastrophic event, it likely contributed to their cultural decline. The Hopewell comet airburst expands our understanding of the frequency and impact of cataclysmic cosmic events on complex human societies.