bluuebunny/crossref_metadata_2025_split huggingface dataset:
Molecular detection and phylogenetic analyses of Anaplasma spp. in Haemaphysalis longicornis from goats in four provinces of China
Authors: Yan Yaqun , Wang Kunlun , Cui Yanyan , Zhou Yongchun , Zhao Shanshan , Zhang Yajun , Jian Fuchun , Wang Rongjun , Zhang Longxian , Ning Changshen 
Published: July 2021

AbstractAnaplasma species, which are distributed worldwide, are gram-negative obligate intracellular tick-borne bacteria that pose a threat to human and animal health. Haemaphysalis longicornis ticks play a vital role as vectors in the transmission of Anaplasma pathogens. However, the Anaplasma species carried by H. longicornis in China are yet to be characterized. In this study, 1074 H. longicornis specimens were collected from goats in four provinces of China from 2018 to 2019 and divided into 371 sample pools. All tick sample pools were examined for the presence of Anaplasma species via nested PCR amplification of 16S ribosomal RNA, major surface protein 4 (msp4), or citric acid synthase (gltA) genes, which were sequenced to determine the molecular and phylogenetic characteristics of the isolates. The overall Anaplasma spp-positive rate of H. longicornis was determined to be 26.68% (99/371). The percentage prevalence of A. phagocytophilum-like1, A. bovis, A. ovis, A. marginale, and A. capra were 1.08% (4/371), 13.21% (49/371), 13.21% (49/371), 1.35% (5/371), and 10.24% (38/371), respectively, and the co-infection rate of two or more types of Anaplasma was 6.47% (24/371). Phylogenetic analyses led to the classification of A. phagocytophilum into an A. phagocytophilum-like1 (Anaplasma sp. Japan) group. Anaplasma bovis sequences obtained in this study were 99.8–100% identical to those of an earlier strain isolated from a Chinese tick (GenBank accession no. KP314251). Anaplasma ovis sequences showed 99.3–99.6% identity to an A. ovis human strain identified from a Cypriot patient (GenBank accession no. FJ460443). Only one msp4 sequence of A. marginale was detected and was grouped with those of other A. marginale isolates, and these A. capra isolates obtained in this present study may be zoonotic. The detection and characterization of four Anaplasma species in H. longicornis in this study have added to the current knowledge of the parasite and provided data on multiple Anaplasma species with veterinary and medical significance from four provinces of China.
Biscogniauxia rosacearum the charcoal canker agent as a pathogen associated with grapevine trunk diseases in Zagros region of Iran
Authors: Bahmani Zeinab , Abdollahzadeh Jafar , Amini Jahanshir , Evidente Antonio 
Published: July 2021

AbstractGrapevine trunk diseases (GTDs) are well-known and significant fungal diseases of Vitis vinifera with a worldwide distribution. During August to November 2016 in a survey to characterize fungi associated with grapevine trunk diseases in Kermanshah Province (west of Iran) vineyards, 286 fungal isolates were obtained. Based on morphology and DNA sequences data eight species were identified, of which Biscogniauxia rosacearum, Neoscytalidium hyalinum and Phaeoacremonium minimum were the most aggressive fungal pathogenic species characterized in this research. N. hyalinum was the most prevalent species. N. hyalinum and Ph. minimum have previously been reported from Vitis vinifera. Thus far, there are two records of Biscogniauxia mediterranea and Biscogniauxia capnodes on grapevine in the world with no data on pathology aspects. To our knowledge, it is the first time B. rosacearum is reported from grapevine across the globe. Pathogenicity test with three strains of B. rosacearum on 2-year-old potted grapevines confirmed the pathogenicity of B. rosacearum on grapevine. The proximity of vineyards to the oak trees in Zagros forests as one of the plant hosts of Biscogniauxia spp. further highlights the need for extensive studies on B. rosacearum as a new fungal pathogen.
Unsteady squeezing flow of Cu-Al2O3/water hybrid nanofluid in a horizontal channel with magnetic field
Authors: Khashi’ie Najiyah Safwa , Waini Iskandar , Arifin Norihan Md , Pop Ioan 
Published: July 2021

AbstractThe proficiency of hybrid nanofluid from Cu-Al2O3/water formation as the heat transfer coolant is numerically analyzed using the powerful and user-friendly interface bvp4c in the Matlab software. For that purpose, the Cu-Al2O3/water nanofluid flow between two parallel plates is examined where the lower plate can be deformed while the upper plate moves towards/away from the lower plate. Other considerable factors are the wall mass suction/injection and the magnetic field that applied on the lower plate. The reduced ordinary (similarity) differential equations are solved using the bvp4c application. The validation of this novel model is conducted by comparing a few of numerical values for the reduced case of viscous fluid. The results imply the potency of this heat transfer fluid which can enhance the heat transfer performance for both upper and lower plates approximately by 7.10% and 4.11%, respectively. An increase of squeezing parameter deteriorates the heat transfer coefficient by 4.28% (upper) and 5.35% (lower), accordingly. The rise of suction strength inflates the heat transfer at the lower plate while the presence of the magnetic field shows a reverse result.
An evolutionary perspective on kin care directed up the generations
Authors: Arnot Megan , Mace Ruth 
Published: July 2021

AbstractWithin evolutionary sciences, care towards younger kin is well understood from an inclusive fitness framework, but why adults would care for older relatives has been less well researched. One existing model has argued that care directed towards elderly parents might be adaptive because of their benefits as carers themselves, with their help freeing up the middle generations’ energy which can then be invested into direct reproduction. However, in this model, elder care is more beneficial to fitness if the carer is fecund. To offer an initial test of this hypothesis, we look at caring behaviour relative to fecundity status in a contemporary dataset from the United Kingdom. If elder care is contingent on possible direct fitness benefits, we would expect women who are still menstruating to care more for their parents than women who can no longer reproduce. Based on this, we also predict that women who are physiologically post-reproductive would invest more in their grandchildren, through whom they can increase their inclusive fitness. After controlling for age and other relevant factors, we find that women who are still menstruating spend more time caring for their parents than those who are not, and the reverse is true when looking at time spent caring for grandchildren. These findings demonstrate that potential inclusive fitness outcomes influence how women allocate care up and down the generations.
Comparison of real-life data of abiraterone acetate and enzalutamide in metastatic castration-resistant prostate cancer
Authors: Demirci Ayşe , Bilir Cemil , Gülbağcı Burcu , Hacıbekiroğlu İlhan , Bayoğlu İbrahim V. , Bilgetekin İrem , Koca Sinan , Çınkır Havva Y. , Akdeniz Nadiye , Gül Deniz , Varım Ceyhun , Demirci Umut , Öksüzoğlu Berna 
Published: July 2021

AbstractTo compare enzalutamide (E) and abiraterone acetate (AA) in terms of efficacy, survival and to characterize prognostic factors affecting survival in metastatic castration-resistant prostate cancer (mCRPC) patients. A total of 250 patients treated with E or AA in 5 centers were included. The number of patients with no prostate specific antigen (PSA) decline was higher in the AA group than that in the E group, and the proportion of patients with a PSA decline of ≥ 50% was higher in the E group (p = 0.020). Radiological progression free survival (rPFS) and overall survival (OS) were significantly longer in the E group when compared to that in the AA group (p < 0.001 and p = 0.027, respectively). In the E group, rPFS was significantly longer than that in the AA group in both pre- and post-docetaxel settings (p = 0.010 and p = 0.003, respectively). OS was similar in the pre-docetaxel setting; but in the post-docetaxel setting, E group had a significantly longer OS than the AA group (p = 0.021). In the multivariate analysis performed in the whole patient group, we found that good prognostic factors for rPFS were E treatment, being ≥ 75 years and a PSA decline of ≥ 50% while there was no factor affecting OS. With longer OS and PFS, E seems to be more suitable for mCRPC patients in the post-docetaxel setting than AA.
Activating transcription factor 4 mediates adaptation of human glioblastoma cells to hypoxia and temozolomide
Authors: Lorenz Nadja I. , Sittig Alina C. M. , Urban Hans , Luger Anna-Luisa , Engel Anna L. , Münch Christian , Steinbach Joachim P. , Ronellenfitsch Michael W. 
Published: July 2021

AbstractThe integrated stress response (ISR) is a central cellular adaptive program that is activated by diverse stressors including ER stress, hypoxia and nutrient deprivation to orchestrate responses via activating transcription factor 4 (ATF4). We hypothesized that ATF4 is essential for the adaptation of human glioblastoma (GB) cells to the conditions of the tumor microenvironment and is contributing to therapy resistance against chemotherapy. ATF4 induction in GB cells was modulated pharmacologically and genetically and investigated in the context of temozolomide treatment as well as glucose and oxygen deprivation. The relevance of the ISR was analyzed by cell death and metabolic measurements under conditions to approximate aspects of the GB microenvironment. ATF4 protein levels were induced by temozolomide treatment. In line, ATF4 gene suppressed GB cells (ATF4sh) displayed increased cell death and decreased survival after temozolomide treatment. Similar results were observed after treatment with the ISR inhibitor ISRIB. ATF4sh and ISRIB treated GB cells were sensitized to hypoxia-induced cell death. Our experimental study provides evidence for an important role of ATF4 for the adaptation of human GB cells to conditions of the tumor microenvironment characterized by low oxygen and nutrient availability and for the development of temozolomide resistance. Inhibiting the ISR in GB cells could therefore be a promising therapeutic approach.
Lipid metabolism of sea urchin Paracentrotus lividus in two contrasting natural habitats
Authors: Anedda Roberto , Siliani Silvia , Melis Riccardo , Loi Barbara , Baroli Maura 
Published: July 2021

AbstractSea urchins Paracentrotus lividus were harvested monthly from April 2015 to March 2016 from two sites in Sardinia (Italy). The two sites, a Posidonia oceanica meadow and a rocky bottom habitat, were naturally characterized by different food sources and availability, being mainly populated by the sea grass Posidonia oceanica and the brown algae Halopteris scoparia, respectively. Total lipids showed a minimum during winter in mature gonads, and a maximum in the summer (recovery stage). Fatty acid (FA) profiles of gut contents and gonads differed from those of the most available food sources. Levels of C18:3 (n-3) (ALA) discriminated samples from the two sites. Despite the very low amounts of C20:5 (n-3) (EPA) and C20:4 (n-6) (ARA) in P. oceanica, the main FA in gonads and gut contents were EPA and ARA in both sites. Increase in green algae intake prior to gametogenesis, especially C. cylindracea, likely affected EPA and ARA levels in gonads. The results show that P. lividus is able to concentrate lipids in gut contents and also to selectively store EPA, ARA and their precursors ALA and 18:2 (n-6) (LA). Moreover, bioconversion of ALA to EPA and of LA to ARA in P. lividus is suggested.
Genetic ancestry inferred from autosomal and Y chromosome markers and HLA genotypes in Type 1 Diabetes from an admixed Brazilian population
Authors: Azulay Rossana Santiago de Sousa , Porto Luís Cristóvão , Silva Dayse Aparecida , Tavares Maria da Glória , Reis Roberta Maria Duailibe Ferreira , Nascimento Gilvan Cortês , Damianse Sabrina da Silva Pereira , Rocha Viviane Chaves de Carvalho , Magalhães Marcelo , Rodrigues Vandilson , Carvalho Paulo Ricardo Vilas Boas , Faria Manuel dos Santos , Gomes Marília Brito 
Published: July 2021

AbstractThis study aimed to investigate the relationship between genetic ancestry inferred from autosomal and Y chromosome markers and HLA genotypes in patients with Type 1 Diabetes from an admixed Brazilian population. Inference of autosomal ancestry; HLA-DRB1, -DQA1 and -DQB1 typifications; and Y chromosome analysis were performed. European autosomal ancestry was about 50%, followed by approximately 25% of African and Native American. The European Y chromosome was predominant. The HLA-DRB1*03 and DRB1*04 alleles presented risk association with T1D. When the Y chromosome was European, DRB1*03 and DRB1*04 homozygote and DRB1*03/DRB1*04 heterozygote genotypes were the most frequent. The results suggest that individuals from Maranhão have a European origin as their major component; and are patrilineal with greater frequency from the R1b haplogroup. The predominance of the HLA-DRB1*03 and DRB1*04 alleles conferring greater risk in our population and being more frequently related to the ancestry of the European Y chromosome suggests that in our population, the risk of T1D can be transmitted by European ancestors of our process miscegenation. However, the Y sample sizes of Africans and Native Americans were small, and further research should be conducted with large mixed sample sizes to clarify this possible association.
Large scale cytokine profiling uncovers elevated IL12-p70 and IL-17A in severe pediatric acute respiratory distress syndrome
Authors: Wong Judith Ju Ming , Tan Herng Lee , Zhou Jieliang , Lee Jan Hau , Leong Jing Yao , Yeo Joo Guan , Lee Yie Hou 
Published: July 2021

AbstractThe specific cytokines that regulate pediatric acute respiratory distress syndrome (PARDS) pathophysiology remains unclear. Here, we evaluated the respiratory cytokine profile in PARDS to identify the molecular signatures associated with severe disease. A multiplex suspension immunoassay was used to profile 45 cytokines, chemokines and growth factors. Cytokine concentrations were compared between severe and non-severe PARDS, and correlated with oxygenation index (OI). Partial least squares regression modelling and regression coefficient plots were used to identify a composite of key mediators that differentially segregated severe from non-severe disease. The mean (standard deviation) age and OI of this cohort was 5.2 (4.9) years and 17.8 (11.3), respectively. Early PARDS patients with severe disease exhibited a cytokine signature that was up-regulated for IL-12p70, IL-17A, MCP-1, IL-4, IL-1β, IL-6, MIP-1β, SCF, EGF and HGF. In particular, pro-inflammatory cytokines (IL-6, MCP-1, IP-10, IL-17A, IL-12p70) positively correlated with OI early in the disease. Whereas late PARDS was characterized by a differential lung cytokine signature consisting of both up-regulated (IL-8, IL-12p70, VEGF-D, IL-4, GM-CSF) and down-regulated (IL-1β, EGF, Eotaxin, IL-1RA, and PDGF-BB) profiles segregating non-severe and severe groups. This cytokine signature was associated with increased transcription, T cell activation and proliferation as well as activation of mitogen-activated protein kinase pathway that underpin PARDS severity.
Correlation of image quality parameters with tube voltage in X-ray dark-field chest radiography: a phantom study
Authors: Sauter Andreas P. , Andrejewski Jana , Frank Manuela , Willer Konstantin , Herzen Julia , Meurer Felix , Fingerle Alexander A. , Makowski Markus R. , Pfeiffer Franz , Pfeiffer Daniela 
Published: July 2021

AbstractGrating-based X-ray dark-field imaging is a novel imaging modality with enormous technical progress during the last years. It enables the detection of microstructure impairment as in the healthy lung a strong dark-field signal is present due to the high number of air-tissue interfaces. Using the experience from setups for animal imaging, first studies with a human cadaver could be performed recently. Subsequently, the first dark-field scanner for in-vivo chest imaging of humans was developed. In the current study, the optimal tube voltage for dark-field radiography of the thorax in this setup was examined using an anthropomorphic chest phantom. Tube voltages of 50–125 kVp were used while maintaining a constant dose-area-product. The resulting dark-field and attenuation radiographs were evaluated in a reader study as well as objectively in terms of contrast-to-noise ratio and signal strength. We found that the optimum tube voltage for dark-field imaging is 70 kVp as here the most favorable combination of image quality, signal strength, and sharpness is present. At this voltage, a high image quality was perceived in the reader study also for attenuation radiographs, which should be sufficient for routine imaging. The results of this study are fundamental for upcoming patient studies with living humans.
Self-assembled Au4Cu4/Au25 NCs@liposome tumor nanotheranostics with PT/fluorescence imaging-guided synergetic PTT/PDT
Authors: Liu Xinyu , Yang Ying , Wang Xueyan , Liu Xuan , Cheng Hanlong , Wang Peisan , Shen Yuhua , Xie Anjian , Zhu Manzhou 
Published: July 2021

A cancer theranostic nanoplatform was successfully assembled by liposome co-loaded with oil-soluble Au4Cu4 and water-soluble Au25 nanoclusters, which presenting excellent performances with multi-imaging/phototherapy effects.
Diagnostic Accuracy of the AzBio Speech Recognition in Noise Test
Authors: Vermiglio Andrew J. , Leclerc Lauren , Thornton Meagan , Osborne Hannah , Bonilla Elizabeth , Fang Xiangming 
Published: July 2021

Purpose
The goal of this study was to determine the ability of the AzBio speech recognition in noise (SRN) test to distinguish between groups of participants with and without a self-reported SRN disorder and a self-reported signal-to-noise ratio (SNR) loss.

Method
Fifty-four native English-speaking young adults with normal pure-tone thresholds (≤ 25 dB HL, 0.25–6.0 kHz) participated. Individuals who reported hearing difficulty in a noisy restaurant (Reference Standard 1) were placed in the SRN disorder group. SNR loss groups were created based on the self-report of the ability to hear Hearing in Noise Test (HINT) sentences in steady-state speech-shaped noise, four-talker babble, and 20-talker babble in a controlled listening environment (Reference Standard 2). Participants with HINT thresholds poorer than or equal to the median were assigned to the SNR loss group.

Results

The area under the curve from the receiver operating characteristics curves revealed that the AzBio test was not a significant predictor of an SRN disorder, or an SNR loss using the steady-state noise Reference Standard 2 condition. However, the AzBio was a significant predictor of an SNR loss using the four-talker babble and 20-talker babble Reference Standard 2 conditions (
p
< .05). The AzBio was a significant predictor of an SNR loss when using the average HINT thresholds across the three Reference Standard 2 masker conditions (area under the curve = .79,
p
= .001).

Conclusions
The AzBio test was not a significant predictor of a self-reported SRN disorder or a self-reported SNR loss in steady-state noise. However, it was a significant predictor of a self-reported SNR loss in babble noise and the average across all noise conditions. A battery of reference standard tests with a range of maskers in a controlled listening environment is recommended for diagnostic accuracy evaluations of SRN tests.
Variable gain switching exact differential observer‐based compensation control for servo system with backlash
Authors: Sun Guofa , Xu Yaming , Wang Yan , Wang Gang 
Published: July 2021

AbstractThis paper proposes a novel variable gain switching exact differential observer (SEDO) to deal with the state estimation of mechanical servo system with unknown backlash. In order to compensate unknown non‐linear backlash accurately, a switching backlash torque mathematic model is constructed to describe the input–output relationships of backlash. Then, based on the switching backlash torque mathematic model, a novel SEDO with variable gain strategy is implemented to compensate the influence of backlash and estimate system states accurately in terms of the change of real operation conditions. Moreover, the designed controller further alleviates the computational burden by conducting a feedforward compensation strategy, part of which is shared by the observer. It is proven that all the signals in the closed‐loop system are bounded, observation error and tracking error are convergent to a small compact set of the origin. Two simulations and two different motor servo test rigs are established to further illustrate the effectiveness of the proposed approach.
KText: Arbitrary shape text detection using modified K‐Means
Authors: Qi Zhuo , Chen Wenyi , Sun Xiaofei , Sun Wangqian , Yang Hui 
Published: July 2021

AbstractText detection methods based on grouping characters have emerged and have achieved promising performance. Nevertheless, previous methods that grouped characters by learning the relation of adjacent characters or used the heuristic clustering method with a handcrafted feature are unsuitable for dense, curved, or long texts. An effective manner of grouping characters is proposed by introducing K‐Means that is modified by the law of universal gravitation, an outlier detection mechanism and sufficient context information. Based on that, corresponding text detector is presented, named the Text Detector, based on modified K‐Means (KText), which can generate the bounding boundary of word‐level texts with an arbitrary shape. In the experimental stage, two novel stratagems are presented to replenish character‐level annotations to several datasets that provide only word‐level annotations. To evaluate the effectiveness of the method, experiments are carried out on three benchmarks, ICDAR2013, ICDAR2015 and Total‐Text, which contain horizontal, oriented and curved text. The results show that KText performs more competently than most state‐of‐the‐art text detectors when handling dense texts with an arbitrary shape.
Polarimetric dynamic vision sensor p(DVS) neural network architecture for motion classification
Authors: Nowak Martin , Beninati Anthony , Douard Nicolas , Puran Alec , Barnes Charles , Kerwick Alexandros , K Giakos George 
Published: July 2021

AbstractThe purpose of this study is to introduce efficient bio‐inspired vision architectures, integrating human cognition capabilities, such as computation and memory emulating neurons and synapses, together with polarization of light properties, that would ultimately revolutionize and give rise to the next‐generation highly efficient augmented intelligence vision systems. The system consists of a polarimetric dynamic vision sensor) and a spinning wheel, whose motion is externally modulated and controlled by means of a signal generator, varying its motion pattern and speed, respectively. A neural network has been designed to classify different object speeds and motion patterns. The neural network utilizes a limited number of events within a given time window, instead of full‐frame images; as well as two classifiers, which practically take a single input and independently classify both its speed and motion pattern. As a result, both high computational efficiency and motion classification accuracy are achieved.