bluuebunny/crossref_metadata_2025_split huggingface dataset:
Chaperonin-Containing TCP1 Subunit 6A Is a Prognostic Potential Biomarker That Correlates With the Presence of Immune Infiltrates in Colorectal Cancer
Authors: Sun Hui , Wang Yan , Jing Hao-Yu , Yang Xin-Yu , Shi Xin-Xiu , Zhang Jia-Hui , Yu Yuan-Xiu , Gao Li , Wang Xin-Yue , Li Wan-Hong , Yu Lei 
Published: May 2021

AimsChaperonin-containing TCP1 subunit (CCT) 6A is an oncogenic 6th subunit of the CCT family. Nevertheless, not much is documented regarding its function in colorectal cancer (COAD). This investigation seeks to explore the role of CCT6A in the prognosis of COAD.Main MethodsSequencing data from the Gene Expression Omnibus (GEO) and Cancer Genome Atlas database (TCGA) were employed to analyze the expression of CCT6A and its involvement in various regulatory networks behind COAD. Oncomine and Gene Expression Profiling Interactive Analysis (GEPIA) analyzed Levels of expression and survival rates, while GEPIA was used to uncover further the functional networks that involved CCT6A. Database for Annotation, Visualization, and Integrated Discovery (DAVID) tools were used to interpret Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathways. Evaluation of the expression levels of CCT6A in COAD samples was also verified via immunohistochemistry.Key FindingsWe found that the expression of CCT6A is up-regulated in COAD. CCT6A correlated with poor prognosis and decreased immune infiltrates such as CD4+ T cells, B cells, and dendritic cells. CCT6A is increased in COAD patients. CCT6A is associated with several gene networks related to the DDX family and mismatch repair pathways.SignificanceOur data showed that data mining was able to uncover data regarding levels of CCT6A and its involvement in genetic regulating pathways in COAD.
Identification and Validation of a Six Immune-Related Genes Signature for Predicting Prognosis in Patients With Stage II Colorectal Cancer
Authors: Li Xianzhe , Xie Minghao , Yin Shi , Xiong Zhizhong , Mao Chaobin , Zhang Fengxiang , Chen Huaxian , Jin Longyang , Lan Ping , Lian Lei 
Published: May 2021

BackgroundImmune-related genes (IRGs) play important roles in the tumor immune microenvironment and can affect the prognosis of cancer. This study aimed to construct a novel IRG signature for prognostic evaluation of stage II colorectal cancer (CRC).MethodsGene expression profiles and clinical data for stage II CRC patients were collected from the Cancer Genome Atlas and Gene Expression Omnibus database. Univariate, multivariate Cox regression, and least absolute shrinkage and selection operator regression were used to develop the IRG signature, namely IRGCRCII. A nomogram was constructed, and the “Cell Type Identification by Estimating Relative Subsets of RNA Transcripts” (CIBERSORT) method was used to estimate immune cell infiltration. The expression levels of genes and proteins were validated by qRT-PCR and immunohistochemistry in 30 pairs of primary stage II CRC and matched normal tissues.ResultsA total of 466 patients with stage II CRC were included, and 274 differentially expressed IRGs were identified. Six differentially expressed IRGs were detected and used to construct the IRGCRCII signature, which could significantly stratify patients into high-risk and low-risk groups in terms of disease-free survival in three cohorts: training, test, and external validation (GSE39582). Receiver operating characteristics analysis revealed that the area under the curves of the IRGCRCII signature were significantly greater than those of the OncotypeDX colon signature at 1 (0.759 vs. 0.623), 3 (0.875 vs. 0.629), and 5 years (0.906 vs. 0.698) disease-free survival, respectively. The nomogram performed well in the concordance index (0.779) and calibration curves. The high-risk group had a significantly higher percentage of infiltrated immune cells (e.g., M2 macrophages, plasma cells, resting mast cells) than the low-risk group. Finally, the results of qRT-PCR and immunohistochemistry experiments performed on 30 pairs of clinical specimens were consistent with bioinformatics analysis.ConclusionThis study developed and validated a novel immune prognostic signature based on six differentially expressed IRGs for predicting disease-free survival and immune status in patients with stage II CRC, which may reflect immune dysregulation in the tumor immune microenvironment.
Clinical Impact of PET With 18F-FDG and 11C-PIB in Patients With Dementia in a Developing Country
Authors: Damian Andres , Portugal Fabiola , Niell Nicolas , Quagliata Adriana , Bayardo Karina , Alonso Omar , Ferrando Rodolfo 
Published: May 2021

Introduction: The objective of this study was to evaluate the clinical impact PET with 18F-FDG and 11C-PIB in patients with dementia in a developing country.Methodology: Retrospective study of the patients referred for the evaluation of dementia to the only PET center in Uruguay. A total of 248 patients were identified, from which 70 patients were included based on the availability of medical history and clinical follow-up. Main outcomes included change in diagnosis, diagnostic dilemma and AD treatment. We evaluated the association of clinical outcomes with PET concordance with baseline diagnosis, diagnostic dilemma, level of education, AD pathology/Non-AD pathology (AD/Non-AD), baseline diagnosis and 11C-PIB PET result.Results: Baseline clinical diagnosis was concordant with 18F-FDG and 11C-PIB PET results in 64.7 and 77.1% of the patients, respectively. Change in diagnosis after PET was identified in 30.0% of the patients and was associated with discordant 18F-FDG (p = 0.002) and 11C-PIB (p &lt; 0.001) PET results, previous diagnostic dilemma (p = 0.005), low education (p = 0.027), Non-AD baseline diagnosis (p = 0.027), and negative 11C-PIB PET result (p &lt; 0.001). Only the last variable remained significant in the multivariate analysis (adjusted p = 0.038). Diagnostic dilemma decreased after PET from 15.7 to 7.1% (p = 0.11) and was associated with Non-AD diagnosis (p = 0.002) and negative 11C-PIB PET result (p = 0.003). Change in AD treatment after PET occurred in 45.7% of the patients.Conclusion:18F-FDG and 11C-PIB PET had a significant clinical impact in terms of change in diagnosis and treatment in patients with dementia in a developing country, similar to that reported in high-income countries.
Neuropsychiatric Symptoms in Parkinson's Disease After Subthalamic Nucleus Deep Brain Stimulation
Authors: Liu Weibing , Yamamoto Tatsuya , Yamanaka Yoshitaka , Asahina Masato , Uchiyama Tomoyuki , Hirano Shigeki , Shimizu Keisuke , Higuchi Yoshinori , Kuwabara Satoshi 
Published: May 2021

Background: Indications for subthalamic nucleus deep brain stimulation (STN-DBS) surgery are determined basically by preoperative motor function; however, postoperative quality of life (QOL) is not necessarily associated with improvements in motor symptoms, suggesting that neuropsychiatric symptoms might be related to QOL after surgery in patients with Parkinson's disease.Objectives: We aimed to examine temporal changes in neuropsychiatric symptoms and their associations with QOL after STN-DBS.Materials and Methods: We prospectively enrolled a total of 61 patients with Parkinson's disease (mean age = 65.3 ± 0.9 years, mean disease duration = 11.9 ± 0.4 years). Motor function, cognitive function, and neuropsychiatric symptoms were evaluated before and after DBS surgery. Postoperative evaluation was performed at 3 months, 1 year, and 3 years after surgery.Results: Of the 61 participants, 54 completed postoperative clinical evaluation after 3 months, 47 after 1 year, and 23 after 3 years. Frontal lobe functions, depression, and verbal fluency significantly worsened 3 years after STN-DBS. Non-motor symptoms such as impulsivity and the Unified PD Rating Scale (UPDRS) part I score were associated with QOL after STN-DBS.Conclusions: Frontal lobe functions, depression, and verbal fluency significantly worsened 3 years after STN-DBS. The UPDRS part I score and higher impulsivity might be associated with QOL after STN-DBS.
The Interplay Between Programmed Death Ligand 1 and Vimentin in Advanced Non-Small-Cell Lung Cancer
Authors: Bronte Giuseppe , Puccetti Maurizio , Petracci Elisabetta , Landi Lorenza , Cravero Paola , Scodes Simona , Ulivi Paola , Ravaioli Sara , Tumedei Maria Maddalena , Burgio Marco Angelo , Cappuzzo Federico , Delmonte Angelo , Crinò Lucio , Bravaccini Sara 
Published: May 2021

BackgroundCurrent therapy for non-small-cell lung cancer (NSCLC) frequently includes immune checkpoint inhibitors, such as pembrolizumab, and programmed death ligand 1 (PD-L1) positivity is mandatory for its use in this setting. Vimentin plays a role in carcinogenesis through the activation of the epithelial-to-mesenchymal transition (EMT) process. Its prognostic impact in NSCLC has been investigated in numerous studies but little data are available on its relation with PD-L1 expression.Patients and MethodsWe retrospectively retrieved data on patients with advanced NSCLC consecutively enrolled in a clinical trial at our institute. PD-L1 and vimentin expression were determined by immunohistochemistry. Correlations between variables were assessed using the Spearman correlation coefficient. The Kaplan-Meier method was used to estimate overall survival (OS) and the Log-rank test was used to compare survival curves. The association between demographic, clinical and biomarker information and survival was investigated with the Cox model.ResultsFifty-three patients were included in the study. A weak positive correlation was observed between the PD-L1 and vimentin (ρ=0.41, P=0.003). Patients with PD-L1 values &lt;1% showed a slightly better OS than those with higher values (HR=2.07; 95% CI: 0.92–4.65), but the difference was not significant (P=0.080). No difference in overall survival (OS) was observed on the basis of vimentin expression (HR=1.25; 95% CI: 0.59–2.66; P=0.554). Patients harboring both vimentin and PD-L1 negative expression (&lt;1%) showed a trend towards better survival than those with ≥1% expression (HR=2.31; 95% CI: 0.87-6.17, P=0.093). No significant associations were observed between gender, age at diagnosis, stage at diagnosis, histology, KRAS or EGFR status, radical surgery or immunotherapy and OS.ConclusionsThe weak positive association between PD-L1 and vimentin suggests a potential interplay between these biomarkers. Further research is warranted to evaluate EMT and immune escape as two components of the same process.
Introduction of a New Pathology Workup Protocol for Glottic Cancer Treated With Transoral Laser Microsurgery (TLM): Prospective Analysis of Oncological Outcomes and Matched Case-Control Study
Authors: Meulemans Jeroen , Narimani Sara , Hauben Esther , Nuyts Sandra , Laenen Annouschka , Delaere Pierre , Vander Poorten Vincent 
Published: May 2021

Background/PurposeThe value of margin status after TLM for glottic cancer is debatable, due to difficulties in specimen orientation and margin analysis. To reduce these difficulties, we recently introduced a standardized protocol of oriented fixation of TLM specimens. This proved feasible and resulted in high margin evaluability rates and a decreased rate of false positive deep margins, when compared to a historical TLM cohort. For the patients whose specimens were processed according to this protocol, we prospectively analyzed oncological outcomes, identified prognostic factors and assessed the influence of the protocol introduction on outcomes compared with a historical TLM cohort.MethodsNinety-six patients with glottic malignancies treated with TLM were included. Resection specimens were processed according to the new protocol. Descriptive statistics and survival analyses were used to determine oncological outcomes. To assess the effect of the protocol introduction on outcomes, a matched-case-control analysis was performed, using a historical TLM-cohort as controls. The Cox proportional hazards model was used to analyze prognostic effects of patient and treatment characteristics, including the pathology protocol introduction, on overall survival (OS), disease-specific survival (DSS), disease-free survival (DFS) and local recurrence-free survival (LRFS).ResultsTwo-year outcomes were favorable: 88.5% OS, 97.0% DSS, and 87.6% LRFS. At multivariable analysis, the presence of multiple positive superficial margins was a negative prognosticator for OS (HR 4.102) and increasing cT classification proved a negative prognosticator for DFS (HR 2.828) and LRFS (HR 2.676). Matched case-control analysis did not reveal a significant difference in oncological outcomes between cohorts. Deep margin status had a strong differential effect for DFS (p-value for interaction = 0.0205) and for LRFS (p-value for interaction = 0.0176) between cohorts, indicating a prognostic effect of deep margin status on both outcomes in the current cohort, but not in the historical cohort.Discussion/ConclusionThe introduction of a new standardized technique of oriented fixation of TLM specimens did not affect oncological outcomes when compared to a historical TLM cohort, but assigned a significant prognostic effect to deep margin status for DFS and LRFS, facilitating the decision making process with regards to planning of second-look procedures, administration of adjuvant radiotherapy or determination of follow-up intensity.
Psychological Symptoms Among Evacuees From the 2016 Fort McMurray Wildfires: A Population-Based Survey One Year Later
Authors: Belleville Geneviève , Ouellet Marie-Christine , Lebel Jessica , Ghosh Sunita , Morin Charles M. , Bouchard Stéphane , Guay Stéphane , Bergeron Nicolas , Campbell Tavis , MacMaster Frank P. 
Published: May 2021

Background: The 2016 wildfires in Fort McMurray (Alberta, Canada) led to a massive displacement of 88,000 people and destroyed 2,400 homes. Although no direct human fatality resulted, many individuals feared for their lives or those of their loved ones.Objectives: (1) To estimate the prevalence of post-traumatic stress, major depressive, insomnia, generalized anxiety, and substance use disorders in the adult population of Fort McMurray 1 year after the evacuation; (2) To identify pre-, peri-, and post-disaster correlates of mental health disorders.Methods: A phone survey using random digit sampling was used to survey evacuees. A total of 1,510 evacuees (response rate = 40.2%, 55.5% women, mean age = 44.11, SD = 12.69) were interviewed between May 9th and July 28th, 2017. Five validated scales were administered: the PTSD Symptoms Checklist (PCL-5), the Insomnia Severity Index (ISI), the depression and anxiety subscales of the Patient Health Questionnaire (PHQ-9, GAD-7), and the CAGE Substance Abuse Screening Tool.Results: One year after the wildfires, 38% had a probable diagnosis of either post-traumatic stress, major depressive, insomnia, generalized anxiety, or substance use disorder, or a combination of these. Insomnia disorder was the most common, with an estimated prevalence of 28.5%. Post-traumatic stress, major depressive and generalized anxiety disorders were almost equally prevalent, with ~15% each. The estimated prevalence of substance use disorder was 7.9%. For all five mental health disorders, having a mental health condition prior to the fires was a significant risk factor, as well as having experienced financial stress or strain due to the economic decline already present in Fort McMurray. Five post-disaster consequences were significant predictors of four of the five disorders: decrease in work, decrease in social life, poorer current health status, increase in drug and alcohol use, and higher level of stress experienced since the fires.Conclusion: One year after the fires, more than one third of the evacuees had clinically significant psychological symptoms, including those of insomnia, post-traumatic stress, depression, anxiety, and substance use. This study helped identify individuals more at risk for mental health issues after a natural disaster and could guide post-disaster psychosocial support strategies.
CHILDHOOD SARCOIDOSIS: A CASE REPORT
Authors: Le Thy Phuong Anh 
Published: May 2021

Childhood sarcoidosis is an uncommon disease and recognition of this disease in children is often delayed because of the lack of awareness and unfamiliarity with its clinical features. With the aim of providing clues for diagnosis and treatment of disease, we reported a a 23-month-old boy hospitalized for multiple pinkish papules and painless cystic swellings in ankles and wrists, diagnosed with sarcoidosis, treated with corticosteroid and well recovered. This case reminds us to include childhood sarcoidosis in the differential diagnosis in pediatric patients who present with multiple papular eruptions along with systemic manifestations. It is characterized by arthritis, uveitis, and cutaneous involvement. The prognosis of early-onset childhood sarcoidosis varies in different studies due to the rarity of the disease. The treatment of choice in systemic involvement of childhood sarcoidosis is corticosteroids.
Key words: sarcoidosis in children, childhood sarcoidosis, granulomatous disorder, arthritis, uveitis.
RELATIONSHIP OF NURSE SERVICE QUALITY WITH PATIENT SATISFACTION IN IN PATIENTROOMS GRANDMED HOSPITAL
Authors: Karokaro Tati Murni , Sitorus Rawaty , Rio Ginting Peni , Lestari Sitepu Abdi , Matua Harahap Safril 
Published: May 2021

Hospital is a health service that has permanent facilities and infrastructure consisting of professional medical personnel to provide health services, continuous nursing care, and diagnose and provide disease treatment to patients in need. The hospital must fulfill superior facilities, have professional human resources, all of which are part of influencing services to provide satisfaction to patients. This study aims to determine the relationship between the quality of nurse services and patient satisfaction in the inpatient room of the GrandMed Hospital, using a descriptive survey research type. The sample of this study was 27 patients in the Ns 4D room using a questionnaire instrument. The sampling technique used was purposive sampling. The results of the research obtained are those who have good quality nurse service 16 people (59.3%) and bad service quality 11 people (40.7%), 17 people who are satisfied (63.0%) and dissatisfied as many 10 people (37.0%), so that the results of the chi square statistical test obtained p value = 0.021, which means that there is a relationship between the quality of nurse services and satisfaction in the 4D patient room of Grandmed Hospital. From the results of this study, the advice that I can give is that nurse can apply the results of this research and make it into consideration for improving nursing services at the hospital not only in the 4D patient room but to all inpatient rooms at GrangMed Hospital. This research can be examined again with a larger number of samples and a larger number of rooms or hospitals in order to see more broadly about the effect of nursing services on patient satisfaction.
ANALISIS KEUNTUNGAN USAHA TERNAK BABI DI KECAMATAN TOMOHON BARAT KOTA TOMOHON (Studi Kasus Pada Usaha Milik Bapak Okny Mende)
Authors: Anes Candy A.A , Massie M T , Lumy T F.D , Sajow A A , Oroh F N,.S 
Published: May 2021

PROFIT ANALYSIS OF PIG FARMING IN WEST TOMOHON DISTRICT TOMOHON CITY. (Case Study On Mr. Okny Mende's Business. The goal of this study was to investigate profit of pig farming in West Tomohon District Survey method was performed on Mr Okny Mende’s farming in Tara Tara 2 Village West Tomohon District bycase study. The pig farmers’ challenge in the production process was the high cost of production. The increase of cost production without profit tend to be problem for farmers because input material (production costs) was very important factors in the livestock business. The Pig farming business has the potential for development in the City of Tomohon due to investment in this business does not require large costs and land, besides that the return on capital is relatively faster and although the development of pig agribusiness is limited due to socio-cultural factors, but in North Sulawesi especially Tomohon City has good prospects because of high demand of local market and the surrounding area, and there are no socio-cultural constraints. The pig farm business located in Taratara Dua Sub-District, West Tomohon District is owned by Mr. Okny Mende's family, which is a nursery business by selling weaned piglets. Results of this study shown that the average total cost incurred per period of production as much as IDR 38.334.531. While revenue per period of production was IDR. 68.400.000. The profit per production period was IDR. 30.065.469.
PENGARUH WADAH MEMASAK YANG BERBEDA TERHADAP SIFAT MUTU ORGANOLEPTIK DAGING AYAM BUMBU RINTEK WUUK (RW)
Authors: Kasih Trifonia , Tinangon Meilani R. , Sembor Sofi M. , Rotinsulu Merri D. 
Published: May 2021

THE EFFECT OF DIFFERENT COOKING CONTAINER ON THE SENSORIC QUALITY OF TRADITIONAL “RINTEK WUUK” (RW) SEASONING CHICKEN. The purpose of this study was to determine the effect of different cooking containers on the organoleptic qualities of RW seasoning chicken meat. This study used a completely randomized design (CRD) with three treatments and 40 replications (untrained panelists). The treatments were arranged as follows: R1 = bamboo container, R2 = earthen pot container, R3 = pan container. The results and further testing of the doubled duncan region showed that different cooking containers gave a real difference (P˂0.05) to the taste but did not give a difference (P˃0.05) to the color, aroma, and texture, but generally accepted by the panelists. Based on data analysis and discussion for all variables it can be concluded that Rintek Wuuk (RW) seasoned chicken meat cooked using bamboo containers is preferred. Keywords: Cooking container, Chicken meat, RW seasoning, Organoleptic
KUALITAS SEMEN SAPI PEJANTAN SIMMENTAL DAN LIMOUSIN YANG DIPELIHARA DALAM TIPE KANDANG YANG BERBEDA DI BALAI INSEMINASI BUATAN LEMBANG
Authors: Lagu Bastiana Elvira , Pudjihastuti Endang , Paputungan Umar , Adiani Sri 
Published: May 2021

SEMEN QUALITY OF LIMOUSIN AND SIMMENTAL BULLS MAINTAINED IN DIFFERENT TYPES OF PENS AT THE INSTITUTION OF LEMBANG ARTIFICIAL. The intensive maintenance of bulls included animal house and pens protecting animals against sunlight, cool effect, rain, high speed of win and other predators. Objective of this study was to evaluate effects of single bull in a pen (SB) and double bulls in a pen (DB) on semen quality of Limousin and Simmental bulls at the Institution of Lembang Artificial Insemination in West Java. Primer and secondary data of both bulls reproduction were used in this study and analyzed by t test method. Results showed that average volume of semen in both bulls of Limousin and Simmental under SB (9.54 ± 2.19 mL/ejaculation) was significantly higher of 7.67 percents than those of bulls under DB (8.86 ± 1.79 mL/ejaculation). The average of percentage of spermatozoa motility in both bulls of Limousin and Simmental under DB (71.00 ± 2.67) was not significantly differ with those of bulls under SB (70.10 ± 2.37). The average of spermatozoa mass movement in both bulls of Limousin and Simmental under SB (1.84 ± 0.37) was significantly more aggressive of 5.43 percents than those of bulls under DB (1.94 ± 0.24). Finally, the average of pH of semen in both bulls of Limousin and Simmental under SB (6.66 ± 0.11) was not significantly differ with those of bulls under DB (6.64 ± 0.10). Correlation between reproduction variables of both Limousinand Simmental bulls in SB and DB was not correlated between one and another variables indicated by correlation values ranging from -0.29 to 0.18. Based of the result of study can be concluded that semen quality of Limousin and Simental in single pen was better than that in double pens. The correlations among reproductive veriables of Limousin and Simmental in both sigle and double pens had no correlation between one and another variable. Keyword: Type of Pen, Semen Quality, Simmental Bull, Limousin Bull.