bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification of lysine acetylome of oral squamous cell carcinoma by label-free quantitative proteomics
Authors: Dong Jingjing , He Jingquan , Zhang Zeyu , Zhang Wei , Li Yixi , Xie Hongliang , Zuo Wenxin , Tang Jianming , Zeng Zhipeng , Cai Wanxia , Lai Liusheng , Yun Manhua , Shen Lingjun , Yin Lianghong , Tang Donge , Dai Yong 
Published: August 2021

Abstract

Background
Lysine acetylation (Kac) favors gene transcription and activates various genes involved in the regulation of oncogenesis, whereas the acetylation profiling of oral squamous cell carcinoma (OSCC) is unknown. We performed lysine acetylation analyses to achieve a comprehensive profile and revealed the specific pathogenesis in patients with OSCC.
Methods
Liquid chromatography − tandem mass spectrometry (LC-MS/MS) was utilized to investigate lysine acetylation features of tumor tissues and adjacent normal tissues from 9 patients with OCSS.
Results
Among the upregulated different acetylation proteins (DAPs), the biological process of GO analysis was closely related to cellular response to regulation of apoptotic process, and regulation of programmed cell death. KEGG enrichment analysis was associated with HIF-1 signaling pathway, ferroptosis, and JAK-STAT signaling pathway. In PPI network, seven differently Kac proteins (SRSF1, HNPNPM, PRPF8, DHX9, DHX15, RBMX, SNRPG) in MCODE1 and the top 30 hub gene involved in mRNA splicing process and spliceosome pathway. Six differently Kac modified proteins of RPS15A, RPL11, RPS11, RPS3, RPL24, RPL19 in MCODE1 was enriched in ribosome pathway, particular lower expression of RPS3, RPL24 and RPL19 were related to the overall survival of OSCC.
Conclusion
This study contributes a foundation for understanding the functions of Kac modification in OSCC and investigates lysine acetylation on proteins involved in ribosome pathway, particularly the ones that acted as hub genes and related to the OSCC survival, which may be a potential therapeutic direction of OSCC in the future.
Partitioning of Benzene in Soil Environment: The Critical Factor of Exposure
Authors: Zhang Ruihuan , Wang Shijie , Jiang Lin , Zhang Wenyu , Fu Quankai , Ma Lin , Zhong Maosheng 
Published: August 2021

Abstract
Background: From the perspective of contaminated sites risk assessment, vapor-solid interface adsorption of volatile organic compounds (VOCs) in soil is thought to play a pivotal role because this greatly affects the release of VOCs into soil gas thus affecting the concentrations of intrusion and human exposure. Understanding the significance requires quantifying different forms of VOCs that occur in soil, but no relevant models have included the vapor-solid interface adsorption yet. Methods: This paper proposes the multiphase partitioning equilibrium (MPE) model that integrates vapor-solid adsorption phase for the first time. The MPE model establishes the quantitative relationship between the total VOC mass in soil and the mass in various partitioning phases. An equilibrium partitioning experiment was designed to verify the validity of the MPE model.Results: The error of the MPE model was basically within an order of magnitude. It also revealed that that the vapor-solid interface adsorption dominates benzene uptake in dry soil, but as reducing adsorption of soil minerals (vapor-solid interface adsorption) by increasing humidity, uptake by partitioning into the soil organic matter (OM) gradually dominates. Soil moisture was demonstrated the controlling factor that affects the partitioning. It was distinct that exposure concentration of benzene in indoor air rises from 0.0013 mg/m3 to 0.0070 mg/m3 when the sandy soil relative saturation increases an order of magnitude from 0.01.Conclusion: This study confirms the significance of VOCs partitioning in soil environment for human health exposure, in addition to frequently-mentioned exposure parameters and building parameters. The MPE model gives accurate mass/concentration prediction of VOCs phases in the soil system, and the relationship between exposure and soil moisture was proved.
Acceptance of COVID-19 Vaccines Among Physicians in Mid Delta Region of Egypt: A Cross-Sectional Study
Authors: Shehata Walaa Mohamed , Elshora Ahmed , Abu-elenin Mira Maged 
Published: August 2021

Abstract
Background Safe and effective vaccines became an important preventive tool to protect people against novel Corona Virus Disease infection. Since physicians are at higher risk for contagion of COVID-19 infection, they were prioritized for early vaccination. Also, they are role models who can improve adherence of people to vaccination. Aim of the study: to determine the acceptability of COVID-19 vaccines among physicians in the Gharbia governorate, Egypt. Methods: A cross-sectional study recruited working physicians through snowballing random sampling technique. Data was collected by using a self-administered electronic questionnaire which measured the self-perceived risk to COVID-19 infection, the attitudes and acceptance of COVID-19 vaccines. Results: The study enrolled 1268 physicians, 22% of them were vaccinated .Only 24% expressed their acceptance towards COVID-19 vaccines, while 36.7% didn't agree to get vaccinated. Vaccination unacceptance was significantly associated with increasing age, female gender, higher educational attainments, limited income, no direct patients' contact, diagnostic subspecialty, and working in a rural health facility (p= 0.004, 0.02, <0.0001, <0.0001, 0.01, 0.01, 0.002 respectively).Conclusions: Though physicians are at higher risk of infection, they had poor acceptance of COVID-19 vaccines which may, induce reluctance of people to get vaccinated. Special actions should be tailored to address the significant mediators associated with vaccine unacceptability.
Molecular Structure, Interactions and Antimicrobial Properties of Curcumin-PLGA Complexes – A DFT Study
Authors: Ganesan Mahendiraprabu , Paranthaman Selvarengan 
Published: August 2021

Abstract
Density functional calculations are performed to study the molecular structure, interactions, and antimicrobial activity of curcumin-poly Lacto glycolic acid (Cur-PLGA) complexes. The calculations are performed on curcumin, Glycolic acid (SSC and AAT conformers), Lactic acid (LA), Cur-SSC, Cur-AAT, Cur-LA, and Cur-PLGA complexes using dispersion corrected M06-2X functional with 6–31 + G* basis set. The condensed Fukui functions of curcumin are calculated to identify the favorable reactive sites. Inter- and intramolecular H–bond interactions are analyzed in detail through natural bond orbital, Atoms in Molecule, and Reduced density gradient analyses. The interaction energy values indicate that the interaction between curcumin and AAT is stronger than the other studied complexes. Further, our calculations show that the PLGA interacted with curcumin is having lower LUMO energy and density values. This indicates that the antimicrobial activity is high in this complex.
Community and Psychological Barriers to Tsunami Preparation
Authors: Lamjiry Zahra Asgarizadeh , Gifford Robert 
Published: August 2021

Abstract
Tsunami risk was investigated as a platform for helping to understand the impact of community and psychological factors in natural disaster preparedness. Residents who lived within four meters of sea level in high and moderate tsunami risk areas of Vancouver Island were queried about possible influences on their preparation. Both community-level and psychological factors played significant roles. More community participation and fewer justifications used for not preparing (the dragons of inaction) directly predicted their reported preparation. The relation between sense of community and preparation was mediated by level of community participation, and the relation between the psychological factor, positive outcome expectancy, and preparation was mediated by the dragons of inaction. Together, these influences accounted for 21 percent of the variance in reported tsunami preparation. Policy implications are discussed.
High-mobility semiconducting polymers with different spin ground states
Authors: Chen Xiao-Xiang , Li Jia-Tong , Fang Yu-Hui , Wang Xue-Qing , Liu Guangchao , Wang Yunfei , Deng Xin-Yu , Gu Xiaodan , Jiang Shang-Da , Lei Ting 
Published: August 2021

Abstract
Organic semiconductors with high-spin ground states are fascinating because they could enable fundamental understanding on spin-related phenomenon in light element and provide opportunities for organic magnetic and quantum materials. Although high-spin ground states have been observed in some quinoidal type small molecules or doped organic semiconductors, semiconducting polymers with high-spin at its neutral ground state are rarely reported because of the less of clear design strategy. Here we propose a molecular design strategy to obtain high-mobility semiconducting polymers with different spin ground states. We show that polymer building blocks with small singlet-triplet energy gap (ΔES−T) could enable small ΔES−T gap and increase the diradical character in copolymers. We first demonstrate that the spin density and solid-state interchain interactions in the high-spin polymers are crucial for their ground states. Polymers with a triplet ground state (S = 1) could exhibit doublet (S = 1/2) behavior due to the solid-state interchain spin-spin interactions. Besides, these polymers showed outstanding charge transport properties with high hole/electron mobilities and can be both n- and p-doped with superior conductivities. Our results demonstrate a rational design approach to high-mobility semiconducting polymers with different spin ground states.
Human Resource Management in Ethiopian Public Hospitals
Authors: Gile Philipos Petros , De Klundert Joris Van , Samardzic Martina Buljac 
Published: August 2021

Abstract
Background

In Ethiopia, public hospitals deal with a persistent human resource crisis, even by SSA standards. Policy and hospital reforms, however, have so far resulted in limited progress towards addressing the resulting strategic human resource management (SHRM) challenges Ethiopia’s public hospital face.
Methods

To explore the contextual factors influencing these SHRM challenges of Ethiopian public hospitals, we conducted a qualitative study based on the SHRM framework of Paauwe. A total of 19 semi-structured interviews were conducted with a purposive sample of hospital CEOs and HR managers from 15 hospitals across Ethiopia. An additional 4 focus group discussions were held with professionals and their line managers.
Results

The study found that hospitals compete on the supply side for scarce resources among which the human resources of skilled professionals. There was little reporting on demand side competition on health services provided, service quality, and service innovation. Governmental regulations were the main institutional mechanism in place. These regulations also emphasized human resources and were perceived to tightly regulate employee numbers, salaries and arrangements at detailed levels. These regulations were perceived to restrict the SHRM autonomy of the hospitals. Regulation induced differences in allowances and external employment arrangements were among the concerns that decreased motivation and job satisfaction and caused employees to leave their jobs. The mismatch between regulation and workforce needs posed challenges for leadership and caused leaders to be perceived as incompetent and unable when not successfully addressing workforce needs.
Conclusions

Bottom-up involvement in SHRM may help resolve the aforementioned persistent problems. The Ethiopian government might better loosen regulations, providing more autonomy to hospitals for SHRM and implement mechanisms that emphasize the quality of health services demanded rather than the quantity of human resources supplied.
TLR4 Agonist and Hypoxia Synergistically Promote the Formation of TLR4/NF-κB/HIF-1α Loop in Human Epithelial Ovarian Cancer
Authors: Guo Xiaoqin , Zhao Bin , Niu Xiulong , Huang Suhui , Yang Jing , Wei Yiyi , Wang Xiujuan , Wang Yue 
Published: August 2021

Abstract
Inflammation and hypoxia are involved in numerous cancer progressions. Reportedly, the toll-like receptor 4 (TLR4)/nuclear factor κappaB (NF-κB) pathway and hypoxia-inducible factor-1α (HIF-1α) are activated and closely related to the chemoresistance and poor prognosis of epithelial ovarian cancer (EOC). However, the potential correlation between TLR4/NF-κB and HIF-1α remains largely unknown in EOC. In our study, the possible positive correlation among TLR4, NF-κB and HIF-1α proteins were investigated in the EOC tissues. Our in vitro results demonstrated that LPS can induce and active HIF-1α through the TLR4/NF-κB signaling in A2780 and SKOV3 cells. Moreover, hypoxia-induced TLR4 expression and the downstream transcriptional activity of NF-κB were HIF-1α dependent. The crosstalk between the TLR4/NF-κB signaling pathway and HIF-1α was also confirmed in nude mice xenograft model. Therefore, we first proposed the formation of a TLR4/NF-κB/HIF-1α loop in EOC. The positive feedback loop enhanced the susceptibility and responsiveness to inﬂammation and hypoxia, which synergistically promote the initiation and progression of EOC. The novel mechanism may act as a future therapeutic candidate for the treatment of EOC.
Exploring the Mechanism of Coagulation Dysfunction in a Sepsis Model though Gene Expression Profiles
Authors: Li Ding , Zhang Xianwei , Zhou Hongmin , Jiang Hao , Sun Yuanyuan , Lu Yilun , Ye Fanrong , Xu Shunyao , Chen Zhiqiang , Pan Jingye 
Published: August 2021

Abstract
Objective. Sepsis is a life-threatening condition, and the mechanism of coagulation dysfunction in sepsis remains unknown. We aimed to investigate the mechanism of coagulation dysfunction in sepsis.Methods. Standard methods were used to establish the sepsis models and generate gene expression profiles. Bioinformatics analysis was carried out by GO and KEGG enrichment analysis, construction of PPIs and screening of seed genes. Finally, seed genes were used to rebuild the disease-related pathways.Results. Our experiments revealed an inflammatory response and coagulation dysfunction in both animal and cell models. After determining the DEGs, GO and KEGG functional analysis showed that there is a significant correlation between the inflammatory response and DNA damage. PPI network analysis screened 9 seed genes related to cell mitosis and platelet-derived growth factor receptor signaling pathways. Some of the seed genes were relevant to COVID-19.Conclusions. This study explored the molecular mechanism of coagulation dysfunction in sepsis models by bioinformatics analysis. This may have guiding significance in reducing the risk of complications in patients with sepsis and improving the effectiveness of treatment.
Obstetric and Neonatal Outcomes Related To Intrapartum Hydration Strategies in Low-Risk Deliveries: An Observational Study
Authors: Hernández-López Ana B. , Muriel-Miguel Cristina , Pérez-Medina Tirso , Morillo Aurora Fernández-Cañadas , López-Lapeyrere Carolina , Salcedo-Mariña Ángel , Fornet-Ruiz Inocencia , Rubio-González Esther , Royuela-Vicente Ana , Solís-Muñoz Montserrat 
Published: August 2021

Abstract
Background

Effective myometrial contractility is important for successful labor, although little attention has been paid to the effect of managing intrapartum fluid intake. Ineffective myometrial contractility leads to prolonged labor, thus increasing obstetric and neonatal adverse outcomes. The risk of prolonged labor can be reduced by increasing the total volume of fluids administered during labor.
Objective

To determine the hydration strategies applied in nulliparous women undergoing low risk labor and their association with obstetric and neonatal outcomes.
Methods

A prospective cohort study was conducted in a Universitary Hospital. The study population included nulliparous women who presented in active labor or induced labor. Sample size was 147. In order to stratify women based on the hydration received, we set as a cut-off point the mean total volume administered per hour (300 ml/h). This enabled to compare obstetric, clinical, and neonatal outcomes in women who had received ≥ 300 mL/h o < 300 mL/h. The primary outcome was total length of labor. Secondary outcomes included maternal and neonatal outcomes.
Results

The study population comprised 148 nulliparous women, mean (DS) age 32.2 (4.4) years, mean (DS) gestational age of 39.4 (1.41) weeks. At admission, median (IQR) dilation was 2 (1–3) cm. Labor was induced in 65.5% (n = 97). Obstetric and neonatal outcomes were more favorable in women who received a ≥ 300 mL/h volume, with statistically significant median differences in the duration total duration of labor (526 vs 735 min; p < 0.001). Clinically relevant differences were also observed with respect to cesarean delivery (14.3% vs 18.7%), fever (5.5% vs 7.7%), weight loss at 24 hours (–2.3% vs − 3%) and at 48 hours (–5.7% vs − 6.3 %), incidence of weight loss > 7% at 48 hours (28.6% vs 39.8%), breastfeeding (94.6% vs 82.4%).
Conclusions

Higher fluid volume administered to nulliparous women during low-risk labor is associated with improved obstetric and neonatal outcomes.
Overexpression of RAB34 Associates With Tumor Aggressiveness and Immune Infiltration in Glioma
Authors: Hou Peng , Wan Quan , Wang Qing , Wu Xuechao , Lu Xiaojie 
Published: August 2021

Abstract
Background: RAB34 is aberrantly expressed in various cancers and exhibits oncogenic properties. However, its function in glioma remains largely unclear. Herein, we investigated the clinical value and biological functions of RAB34 in glioma.Methods: In this study, we collected 697 RNA-seq data from The Cancer Genome Atlas (TCGA) dataset and 325 RNA-seq data from Chinese Glioma Genome Atlas (CGGA) dataset. CCK-8 and EdU assays were employed to assess cell proliferation ability. The transwell assay was utilized to explore cell migration and invasion capacities. Western blot coupled with qRT-PCR were employed to determine the protein, as well as RNA contents. The statistical analyses along with the graphical work were mainly implemented in the R software.Results: RAB34 expression was positively related to the glioma tumor grade, and predicted poor outcomes for glioma patients. RAB34 expression was significantly upregulated in classical and mesenchymal subtypes, and IDH wild-type gliomas. Additionally, RAB34 expression was regulated by promoter DNA methylation. Moreover, RAB34 expression was remarkably correlated with inflammatory activities, immune infiltration, and immune checkpoints in glioma. In vitro experiments demonstrated that inhibition of RAB34 restrained the growth, migration, as well as invasion of glioma cells, and reversed the epithelial-to-mesenchymal transition (EMT) process. Conclusion: Our findings established RAB34 as a novel progression-related biomarker and a possible immunotherapy target for glioma.
Spatial Evolution Path of Historical International Community from the Perspective of Actor Network: A Case Study of Gulangyu Island
Authors: Li Yuan , Guo Jing , Zhao Long , Chen Yiping , Wang Cheng , Wang Cheng , Li Jonathan 
Published: August 2021

Abstract
Gulangyu Island is a special case of social development and changes since modern China. In the past, Chinese and foreign people lived together and Chinese and Western cultures coexisted, resulting in an international community with outstanding cultural diversity and modern quality of life. As a functional carrier, space is of great reference significance to the research on the spatial evolution path of Gulangyu international community with the accelerating globalization and rapid expansion of urbanization. Based on the interpretation of historical maps and the integration of historical geographic information, this paper explores the evolution path of Gulangyu community space in modern times from the perspective of actor network theory. It is found that: (1) The change of key actors promotes the spatial evolution of Gulangyu international community. (2) In the stage of foreign culture dissemination (1840-1902), foreign nationals, as key actors, promoted the formation and development of actor networks through administrative recruitment and other means, and promoted Gulangyu Island to change from a traditional Minnan community to a modern community form, with the embryonic form of an international community beginning to emerge. (3) In the stage of multicultural integration (1903-1940), overseas Chinese returning home became key actors. The concept of combination of Western cultures was not only embodied in community management, but also acted on space practice to promote the multicultural integration of Gulangyu international community. This paper summarizes the effects of various factors on the evolution of community space, in order to provide reference for other coastal cities to rationally develop islands and promote the multicultural integration of local communities.
Land Use Suitability Analysis for Sustainability of Water Resources
Authors: Dönmez Nazlı Yıldız , Aktaş Nilüfer Kart 
Published: August 2021

Abstract
Cities are developing and spreading in an unplanned way with urbanization and rapid population growth. Natural resources such as forests and agricultural lands and water resources are the primary sources that are changed and consumed due to urbanization. This situation poses serious threats especially on drinking water basins. The aim of this study is to identify the threats of urbanization pressure on the basins, to analyze the changing landscapes and to determine suitable areas for settlement and agricultural areas for the sustainability of the basin. Sapanca Basin, chosen as the study area, is a crucial drinking water basin for Turkey. The reason for choosing this area is that the basin is under the pressure of construction as a result of the increasing population and secondary housing demand in recent years, and this situation shows a visible change in the landscapes of the basin. In this context, suitability analyzes were made for the determination of settlement and agricultural areas in order to ensure the ecological sustainability of the basin and to give a direction of the development of the basin. These analyzes have been made by considering McHarg's suitable land use method in ecological planning. For suitability analysis, ecological data have been synthesized and suitability maps have been formed by overlay method in GIS environment. Land use maps were produced for the basin and these maps were compared with the environmental plan. It has been determined that a large part of the basin is not suitable for settlement. As a result, it has been determined that the residential areas in the basin increased by 200% between the years 2000-2018. This increase was especially on forest areas and agricultural areas. As a result of these changes, the landscape is fragmented and its natural ecosystem is deteriorated. With the study, the current situation of settlement and agricultural areas in the basin was interpreted and suggestions were developed on how these areas should develop in the basin.