bluuebunny/crossref_metadata_2025_split huggingface dataset:
The DNA methylation landscape of five pediatric-tumor types
Authors: Parker Alyssa C. , Quinteros Badi I. , Piccolo Stephen R. 
Published: August 2021

AbstractFewer DNA mutations have been identified in pediatric tumors than adult tumors, suggesting that alternative tumorigenic mechanisms, including aberrant DNA methylation, may play a prominent role in pediatric tumors. Methylation is an epigenetic process of regulating gene expression in which methyl groups are attached to DNA molecules, often in promoter regions. In Wilms tumors and acute myeloid leukemias, increased levels of epigenetic silencing have been associated with worse patient outcomes. However, to date, researchers have studied methylation primarily in adult tumors and for specific genes but not on a pan-pediatric cancer scale. We addressed these gaps first by aggregating methylation data from 309 noncancerous samples and establishing baseline expectations for each gene. Even though these samples represent diverse tissue types and population ancestral groups, methylation levels were highly consistent for most genes. Second, we compared tumor methylation levels against these baseline values for five pediatric cancer types—Wilms tumors, clear cell sarcomas of the kidney, rhabdoid tumors, neuroblastomas, and osteosarcomas. Hypermethylation was more common than hypomethylation—as many as 11.8% of genes were hypermethylated in a given tumor, compared to a maximum of 4.8% for hypomethylated genes. For each cancer type, genes with the highest variance exhibited consistently divergent methylation patterns for distinct patient subsets. We evaluated whether genomic and epigenomic abnormalities contribute to pediatric tumorigenesis in a mutually exclusive manner but did not find evidence of this phenomenon. Furthermore, even though oncogenes are commonly upregulated in tumors, and tumor-suppressor genes are commonly downregulated in tumors, we did not find statistical evidence that methylation drives such patterns on a broad scale in pediatric tumors.
Mis-regulation of the Nucleoporins 98 and 96 lead to defects in protein synthesis that promote hallmarks of tumorigenesis
Authors: Pulianmackal Ajai J. , Kanakousaki Kiriaki , Flegel Kerry A. , Grushko Olga G. , Gourley Ella , Rozich Emily , Buttitta Laura A. 
Published: August 2021

AbstractThe Nucleoporin 98KD (Nup98) is one of the most promiscuous translocation partners in hematological malignancies, contributing to at least 31 different truncation-fusion proteins. To date, nearly all disease models of Nup98 translocations involve ectopic expression of transgenes recapitulating the fusion protein under study, leaving the endogenous Nup98 loci unperturbed. Overlooked in these approaches is that translocation leads to the loss of one copy of normal Nup98 in addition to the loss of Nup96 – a second Nucleoporin encoded within the same mRNA and reading frame as Nup98. Nup98 and 96 are also mutated in a number of other cancer types and are located near a tumor suppressor region known to be epigenetically silenced, suggesting that their disruption is not limited to blood cancers. We found that reducing Nup98-96 function via an RNAi approach in Drosophila melanogaster (where the Nup98-96 shared mRNA and reading frame gene structure is conserved) de-regulates the cell cycle. We find evidence of over-proliferation in Nup98-96 deficient tissues, counteracted by elevated apoptosis and aberrant Wingless and JNK signaling associated with chronic wound healing. When the knockdown of Nup98-96 is combined with inhibition of apoptosis, we see synergism leading to dramatic tissue overgrowth, consistent with a tumor-suppressor function for endogenous Nup98 and 96. To understand how growth and proliferation become mis-regulated when Nup98-96 levels are reduced, we performed RNAseq and uncovered a gene expression signature consistent with defects in ribosome biogenesis. We found that reducing Nup 98 and 96 function limits nuclear export of the ribosome component RpL10A, leading to defects in protein synthesis. Defects in protein synthesis are sufficient to trigger JNK signaling that contributes to compensatory proliferation and hallmarks of tumorigenesis when apoptosis is inhibited. Based upon our data, we suggest that the partial loss of Nup98 and Nup96 function in translocations could de-regulate protein synthesis leading to stress signaling that cooperates with other mutations in cancer to promote tumorigenesis.HighlightsCompromising Nups 98 and 96 triggers cell death and compensatory proliferation via JNK signaling that becomes tumorigenic when apoptosis is blockedReducing Nup 98 and 96 function limits nuclear export of the ribosome stalk component RpL10A, leading to defects in protein synthesis which cause stress signaling via JNK.Reduced protein synthesis coupled with increased JNK signaling, paradoxically leads to more rapid proliferation with a gene expression signature that resembles a chronic wounding response.Overexpression of Nup98, which occurs in oncogenic fusions, leads to similar defects in protein synthesis and JNK activation.
High temperature perception in leaves promotes vascular regeneration in distant tissues
Authors: Serivichyaswat Phanu T. , Bartusch Kai , Leso Martina , Musseau Constance , Iwase Akira , Chen Yu , Sugimoto Keiko , Quint Marcel , Melnyk Charles W. 
Published: August 2021

AbstractCellular regeneration in response to wounding is fundamental to maintain tissue integrity. Various internal factors including hormones and developmental pathways affect wound healing but little is known about how external factors influence regeneration. To better understand how the environment affects regeneration, we investigated the effects of temperature using the horticulturally relevant process of plant grafting. We found that elevated temperatures accelerated vascular regeneration of Arabidopsis thaliana and tomato (Solanum lycopersicum) grafts. Leaves were critical for this effect since blocking auxin transport or mutating PHYTOCHROME INTERACTING FACTOR4 (PIF4) or YUCCA2/5/8/9 in the cotyledons abolished the temperature enhancement. However, these perturbations had no effect upon graft healing at ambient temperatures and mutations in PIF4 did not affect the temperature enhancement of callus formation or tissue adhesion, suggesting that leaf-derived auxin was specific for enhancing vascular regeneration in response to elevated temperatures. Tissue-specific perturbations of auxin response using a BODENLOS (BDL) mutant revealed an asymmetric effect of temperature upon regeneration: the presence of bdl above the cut prevented temperature enhancement whereas the presence of bdl below the cut prevented graft healing regardless of temperature. Promotion of tissue regeneration by elevated temperatures was not specific for graft healing and we found that elevated temperatures accelerated xylem formation between the parasite Phtheirospermum japonicum and host Arabidopsis thaliana, and this effect required shoot-derived auxin from the parasite. Taken together, our results identify a pathway by which elevated temperatures accelerate vascular development which could be of relevance for improving regeneration and better understanding inter-plant vascular connections.
SARS-CoV-2 B.1.351 (beta) variant shows enhanced infectivity in K18-hACE2 transgenic mice and expanded tropism to wildtype mice compared to B.1 variant
Authors: Tarrés-Freixas Ferran , Trinité Benjamin , Pons-Grífols Anna , Romero-Durana Miguel , Riveira-Muñoz Eva , Ávila-Nieto Carlos , Pérez Mónica , Garcia-Vidal Edurne , Pérez-Zsolt Daniel , Muñoz-Basagoiti Jordana , Raïch-Regué Dàlia , Izquierdo-Useros Nuria , Blanco Ignacio , Noguera-Julián Marc , Guallar Victor , Lepore Rosalba , Valencia Alfonso , Vergara-Alert Júlia , Clotet Bonaventura , Ballana Ester , Carrillo Jorge , Segalés Joaquim , Blanco Julià 
Published: August 2021

AbstractSARS-CoV-2 variants display enhanced transmissibility and/or immune evasion and can be generated in humans or animals, like minks, thus generating new reservoirs. The continuous surveillance of animal susceptibility to new variants is necessary to predict pandemic evolution. In this study we demonstrate that, compared to the B.1 SARS-CoV-2 variant, K18-hACE2 transgenic mice challenged with the B.1.351 variant displayed a faster progression of infection. Furthermore, we also report that B.1.351 can establish infection in wildtype mice, while B.1 cannot. B.1.351-challenged wildtype mice showed a milder infection than transgenic mice, confirmed by detectable viral loads in oropharyngeal swabs and tissues, lung pathology, immunohistochemistry and serology. In silico models supported these findings by demonstrating that the Spike mutations in B.1.351 resulted in increased affinity for both human and murine ACE2 receptors. Overall, this study highlights the plasticity of SARS-CoV-2 animal susceptibility landscape, which may contribute to viral persistence and expansion.
Corrosion behavior in Ringer solution of several commercially used metal alloys
Authors: Garcia-Falcon Carmen Marina , Gil-Lopez Tomas , Verdu-Vazquez Amparo , Mirza-Rosca Julia Claudia 
Published: August 2021

Purpose
This paper aims to analyze the corrosion behavior in Ringer solution of six commercially used Ni-based alloys that are present and commonly used as metallic biomaterials.

Design/methodology/approach
The specimens were received in the form of cylindrical ingots and were cut to get five samples of each brand with a cylindrical shape of 2 mm height to conduct the study. In this scientific research, the following techniques were used: open circuit potential, potentiodynamic polarization studies, and electrochemical impedance spectroscopy.

Findings
The study findings revealed the passivation tendency of the different specimens. Additionally, when the materials were compared, it was discovered that the decisive factor for high corrosion resistance was the chromium concentration. However, with similar chromium content, the stronger concentration in molybdenum increased the resistance. According to the results obtained in this investigation, the biological safety of the dental materials studied in Ringer solution was considered very high for specimens 1 and 2, and adequate for the other samples.

Originality/value
Metal alloys used as biomaterials in contact with the human body should be deeply investigated to make sure they are biocompatible and do not cause any harm. The corrosion resistance of an alloy is the most important characteristic for its biological safety, as all problems arise because of the corrosion process. There is scarce investigation in these Ni-based dental biomaterials, and none found in these commercially used dental materials in Ringer solution.
Food delivery apps (FDAs) in Asia: an exploratory study across India and the Philippines
Authors: Pandey Shweta , Chawla Deepak , Puri Sandeep 
Published: August 2021

PurposeThis study explores and compares the reasons for or against including situational triggers on the adoption of food delivery apps (FDAs) across lesser-researched Asian countries like India and the Philippines.Design/methodology/approachThe study utilises a qualitative research methodology using focus group discussions and interviews across each country.FindingsRespondents from India highlighted the importance of convenience, aggressive discounts, app service quality, fulfilment and multiple payment options as the key reasons for adopting the FDAs. However, these factors require redressal in the Philippines context. Also, the study findings highlight some country-specific requirements: in the Philippines, food packaging, cheaper options provided by the local convenience stores and lack of variety of food options; in India, the impact of parental beliefs and segregated vegetarian food delivery.Practical implicationsThe study makes practical suggestions for consumer behaviour researchers, developers and marketers of FDAs who are confronted with challenges in app development for a multi-cultural audience and to ensure growth and the adoption of the FDAs across the two countries.Originality/valueThis is one of the first studies to compare diverse national markets to uncover differences and similarities in the factors impacting the adoption of FDAs. The results highlight the impact of varying levels of evolution of FDAs and social, technological and cultural contextual differences on the adoption of the FDAs across India and the Philippines.
Music festival supervisor leadership style and organizational citizenship behavior: the effects of employee and volunteer relationships and dependence on their leader
Authors: Sisson Annamarie D. 
Published: August 2021

PurposeThe relationship between leadership style and employee outcomes is well established. However, organizational management research lacks an understanding of the dyadic relationship between supervisors and employees and volunteers at music festivals. This study examines a mediation model in which leader–member exchange (LMX) affects leadership style and organizational citizenship behavior. Additionally, the study examines a moderating model in which dependence on the leader moderates relationships between leadership style and leader–member exchange.Design/methodology/approachConfirmatory factor analysis, structural equation modeling and conditional process analysis were employed to test the model using data collected from 97 supervisors, volunteers and employees who worked at an annual large-scale music festival staged in Costa Rica.FindingsResults reveal that leadership style relates positively to LMX and organizational citizenship behavior (OCB). Simultaneously, the mediating effect of LMX and moderating effect of dependence on the leader was not significant on the leadership style and OCB.Research limitations/implicationsGiven the small sample size and accessibility to one music festival, caution should be taken in drawing causal conclusions from the results.Practical implicationsThe study contributes to the knowledge of event and festival management with recommendations for leadership training initiatives for supervisors and employees/volunteers.Originality/valueThis study is among the first to examine the mediating role of LMX between leadership style and OCB, moderating the role of dependence on leadership style and LMX with music festival supervisors and employees/volunteers.
An integrated model of supply chain resilience and its impact on supply chain performance under disruption
Authors: Juan Shih-Jung , Li Eldon Y. , Hung Wei-Hsi 
Published: August 2021

PurposeThis study aims to explore the relationships among the five components of supply chain (SC) resilience (SCRES): visibility, velocity, flexibility, robustness and collaboration and their impacts on the SC performance under disruption (SCPUD).Design/methodology/approachFive SCRES components are identified from the literature review and data are collected using an web survey from 113 manufacturing companies in Taiwan. The data are analyzed by structured equation modeling with the partial least square solution. Two-stage least-squares (2SLS) regression was used to test the potential endogeneity of SC collaboration (SCC).FindingsThe results reveal that SCC is an exogenous driver of SCRES; it directly affects visibility, velocity, flexibility, robustness and SCPUD. Furthermore, SC flexibility is the only component of SC agility that directly affects SCPUD; it is influenced directly by SC velocity and indirectly by SC visibility through SC velocity. SC visibility is a vital agility component that positively influences SC velocity and SC robustness.Research limitations/implicationsThe data in this study are cross-sectional and the sample size of 113 is relatively small. The relationship between SC robustness and SCPUD needs a longer observation period to reveal. The logistic issue in the shortage of carriers caused by the pandemic has been overlooked.Practical implicationsA firm should enhance its collaboration and flexibility in the SC as they both are the critical antecedents of SC performance (SCP) during the disruption period.Originality/valueThis study integrates visibility, velocity, flexibility, robustness and collaboration into a complete framework of SCRES. The dependent variable, SCPUD, measures SC performance (SCP) under the disruption caused by the COVID-19 pandemic. It is the first study to investigate the associations of the six constructs in a research model.
Informal governance in the digital transformation
Authors: Keller Jakob , Burkhardt Patricia , Lasch Rainer 
Published: August 2021

PurposeThe purpose of this study is to explore if and how informal governance mechanisms are used for coordinating actors of digitalized supply networks.Design/methodology/approachA multiple case study analysis of ten German firms in digitalized supply networks is conducted. Data are collected through semi-structured expert interviews with interviewees having notable years of professional experience in purchasing or sales.FindingsThis research shows that reduced personal contacts in digitalized supply networks decrease the ability to coordinate with informal governance mechanisms. However, the need for informal governance in digitalized supply networks remains when facing supply network disruptions or building new business relationships.Originality/valueThis is the first study examining the role of informal governance mechanisms in digitalized supply networks. Theoretical and practical insights on the changing abilities and needs to use informal governance after the digital transformation are given.
The resurgence of nationalism and its implications for supply chain risk management
Authors: Charpin Remi 
Published: August 2021

PurposeThis paper examines nationalism as a driver of political risk and how it can lead to supply chain disruptions for foreign multinational enterprises (MNEs).Design/methodology/approachConceptual research based on a review of the literature on nationalism and supply chain risk management.FindingsThis research unveils how economic nationalism could engender supply chain disruptions via discriminatory practices toward all foreign MNEs and how national animosity may generate additional risks for the MNEs of nations in conflict with one another. These discriminatory practices include an array of host government and grassroots actions targeting foreign MNEs. While economic nationalism and national animosity emanate from within a host country, they may stimulate geopolitical crises outside the host country and thereby affect the international supply chains of foreign MNEs.Research limitations/implicationsThis research lays the foundation for analytical and empirical researchers to integrate key elements of nationalism into their studies and recommends propositions and datasets to study these notions.Practical implicationsThis study shows the implications that nationalist drivers of supply chain disruptions have for foreign MNEs and thus can help managers to proactively mitigate such disruptions.Originality/valueThis study reveals the importance of integrating notions of national identity and national history in supply chain research, since they play a key role in the emergence of policies and events responsible for supply chain disruptions.
The impact of coronavirus and quarantine on Australian public sector organizations
Authors: AlMazrouei Hanan 
Published: August 2021

PurposeThe COVID-19 pandemic has challenged leaders to alter the way they manage their organization's employees, such as requiring them to quarantine, self-isolate or practice social distancing so that they comply with government health directives. The purpose of this study is to investigate the impact of the quarantine on the workplace.Design/methodology/approachStructured interviews containing open-ended questions were conducted with fifty Australian public sector executive managers to gauge the effect of the COVID-19 pandemic on their organizations.FindingsThe study reveals the effects that the virus had on the organizations' teamwork as well as individuals' learning. It finds that organizations experienced both positive effects, such as increased creativity and initiative, and negative effects, such as reduced satisfaction with work teams, from the pandemic.Originality/valueThis study also adds to the still-developing body of knowledge regarding the effects of the virus on individuals' levels of work team satisfaction as well as a variety of other factors specific to public sector organizations
Provision of education for sustainability development and sustainability literacy in business programs in three higher education institutions in Brazil, Colombia and Peru
Authors: Cavalcanti-Bandos Melissa Franchini , Quispe-Prieto Silvia , Paucar-Caceres Alberto , Burrowes-Cromwel Toni , Rojas-Jiménez Héctor Heraldo 
Published: August 2021

PurposeThis paper aims to report on the status and the development of education for sustainable development (ESD) and sustainability literacy (SL) in three Latin American (LA) higher education institutions (HEIs) business programs in Peru, Brazil and Colombia. The paper examines institutional efforts to both introduce and implement ESD curricula and provide SL.Design/methodology/approachThe methods used in this paper included reviews of university Web pages and course materials. Structured interviews were also conducted with program leaders, to examine the level of ESD, as input affecting the business programs curricula of the universities concerned.FindingsInitial findings suggest that, in the three HEIs surveyed, there is still a tendency to talk about issues related to ESD but actions that confirm this interest are not sufficiently advanced. The authors surveys a sample of business programs curricula and interviewed its leaders and a mixed and dated picture emerged. When compared to other regions particularly the USA and Europe, the findings show that the HEIs surveyed still have not developed enough work to distinguish conceptually between sustainable development, ESD and SL making the embedment of these concepts in the curriculum not fully developed.Originality/valueIn LA HEIs, the ESD message seems to be slowly taking ground, equipping HEIs to respond to SL concerns. Implementation and practice in some HEIs are still at an embryonic and conceptually confused stage with regard to LA HEIs SL. This paper sheds light to help ESD delivery. It offers some strategies for moving on from this inception phase to a more structured SL provision and ESD outlook.
Millennial behavioural intention in Islamic banks: the role of social media influencers
Authors: Dean David , Suhartanto Dwi , Pujianti Ferty Nadya 
Published: August 2021

Purpose
This study aims to examine the role of social media influencers (SMI) in millennial behavioural intentions towards Islamic banks, from the perspective of both bank clients and non-clients.

Design/methodology/approach
Of the 484 Indonesian millennials in the sample, 278 were clients and 206 non-clients of Islamic banks. Factor analysis was used to examine the SMI dimension in the Islamic banking context. This study then used the partial least square to evaluate the proposed model and test the relationships between SMI, bank image, trust and behavioural intention.

Findings
Three SMI dimensions were confirmed, i.e. expertise, celebrity and similarity. For bank clients, the findings show that SMI has a significant influence on behavioural intention and reinforces bank image and trust. For non-clients, SMI does not have a significant impact on behavioural intentions but significantly strengthens bank image and trust.

Practical implications
Islamic bank managers can take benefit of this study findings by learning to foster the behavioural intentions of their millennial clients and non-clients using SMIs. Further, choosing the right SMIs for their Islamic bank is an important activity, and if they want to make a positive impact on existing and potential millennial clients, they need to choose popular millennials who are knowledgeable about the Islamic value compliance of Islamic banking services.

Originality/value
This study is an early study to explore SMI’s role in influencing the behavioural intentions of millennials towards Islamic banks.