bluuebunny/crossref_metadata_2025_split huggingface dataset:
Surgical treatment for post-infarction papillary muscle rupture: a multicentre study
Authors: Massimi Giulio , Ronco Daniele , De Bonis Michele , Kowalewski Mariusz , Formica Francesco , Russo Claudio Francesco , Sponga Sandro , Vendramin Igor , Falcetta Giosuè , Fischlein Theodor , Troise Giovanni , Trumello Cinzia , Actis Dato Guglielmo , Carrozzini Massimiliano , Shah Shabir Hussain , Coco Valeria Lo , Villa Emmanuel , Scrofani Roberto , Torchio Federica , Antona Carlo , Kalisnik Jurij Matija , D’Alessandro Stefano , Pettinari Matteo , Sardari Nia Peyman , Lodo Vittoria , Colli Andrea , Ruhparwar Arjang , Thielmann Matthias , Meyns Bart , Khouqeer Fareed A , Fino Carlo , Simon Caterina , Kowalowka Adam , Deja Marek A , Beghi Cesare , Matteucci Matteo , Lorusso Roberto 
Published: October 2021

Abstract OBJECTIVESPapillary muscle rupture (PMR) is a rare but potentially fatal complication of acute myocardial infarction. The aim of this study was to analyse the patient characteristics and early outcomes of the surgical management of post-infarction PMR from an international multicentre registry.METHODSPatients underwent surgery for post-infarction PMR between 2001 through 2019 were retrieved from database of the CAUTION study. The primary end point was in-hospital mortality.RESULTSA total of 214 patients were included with a mean age of 66.9 (standard deviation: 10.5) years. The posteromedial papillary muscle was the most frequent rupture location (71.9%); the rupture was complete in 67.3% of patients. Mitral valve replacement was performed in 82.7% of cases. One hundred twenty-two patients (57%) had concomitant coronary artery bypass grafting. In-hospital mortality was 24.8%. Temporal trends revealed no apparent improvement in in-hospital mortality during the study period. Multivariable analysis showed that preoperative chronic kidney disfunction [odds ratio (OR): 2.62, 95% confidence interval (CI): 1.07–6.45, P = 0.036], cardiac arrest (OR: 3.99, 95% CI: 1.02–15.61, P = 0.046) and cardiopulmonary bypass duration (OR: 1.01, 95% CI: 1.00–1.02, P = 0.04) were independently associated with an increased risk of in-hospital death, whereas concomitant coronary artery bypass grafting was identified as an independent predictor of early survival (OR: 0.38, 95% CI: 0.16–0.92, P = 0.031).CONCLUSIONSSurgical treatment for post-infarction PMR carries a high in-hospital mortality rate, which did not improve during the study period. Because concomitant coronary artery bypass grafting confers a survival benefit, this additional procedure should be performed, whenever possible, in an attempt to improve the outcome.Clinical trial registrationclinicaltrials.gov: NCT03848429.
Inside—and outside—Illibata: composition, context and chronology in a famous Josquin motet
Authors: Rifkin Joshua 
Published: October 2021

Abstract
Opinions remain divided on the date of Illibata Dei virgo nutrix, the motet with the famous acrostic spelling the name Josquin des Prez. Some writers see it as a product of the composer’s years at the papal chapel, 1489–94; others would move its origin considerably earlier, even to 1466–75. The only real foothold we have comes from a Vatican copy made in the mid-1490s. Advocates for an early date see the music as particularly close to the works of Du Fay, Busnoys and Johannes Regis; those supporting a Roman origin point to a trend there during Josquin’s tenure towards similarly ‘archaic’ motets. In both instances, the writers have sought a context in the music of other composers. The present study turns the focus inward to seek a context within the development of Josquin himself. Despite the uncertainty that scholars find in Josquin chronology at every turn, careful analysis of compositional procedure offers a way of breaking the apparent logjam over what everyone recognizes as one of the most significant works of its era.
Using Risk–Benefit Analysis to Control Salmonella in Chicken Meat
Authors: Tondo Eduardo Cesar , Gonçalves Claudia Titze Hessel 
Published: October 2021

Abstract
We have created a risk–benefit analysis (RBA) model to assist in food safety decision-making by analyzing Salmonella control in Brazilian chicken meat. First, we described the issues in a risk profile and used a 5×5 matrix to rank the risks associated with Salmonella. We then classified the magnitude of benefits and costs of control measures using another matrix. Finally, we verified the beneficial effects of recommended control measures using Quantitative Microbiological Risk Assessment (QMRA). The RBA classified Salmonella contamination as risk 6, indicating that control measures should be taken in the short and medium terms. It also recommended the adoption of biosecurity measures on farms to reduce the prevalence of Salmonella in birds, better control of carcass washings and chiller tank management, and information placement on packages and campaigns to raise the awareness of the population about the need to control Salmonella contamination before consumption. On the other hand, it did not recommend better controls at scalding and defeathering. QMRA confirmed the beneficial effects of the recommended control measures. For example, as Salmonella prevalence in poultry increased from 4.04 per cent to 50 per cent, the risk of infection per serving also increased from 0.0080 to 0.071. Although better controls in washings and chiller tank management did not affect the risk of infection, it reduced Salmonella counts on carcasses. We assume that the presence of Salmonella on carcasses was due to improper thermal processing or cross-contamination, which increased the risk from 0.0080 to 0.015962. The RBA demonstrated the logic involved in the adoption of control measures, and this can be helpful in the risk management of food safety issues.
One stomach, two subtypes of carcinoma—the differences between distal and proximal gastric cancer
Authors: Zhang Yuan , Zhang Peng-Shan , Rong Ze-Yin , Huang Chen 
Published: October 2021

Abstract
Gastric cancer (GC) is one of the most common malignant tumors of the digestive tract, posing a significant risk to human health. Over the past 10 years, the pathological characteristics and the prognosis of GC have been determined based on the locations of the tumors that were then classified into two types—proximal and distal GC. This review focuses on the differences in epidemiology, etiology, cell source, pathological characteristics, gene expression, molecular markers, manifestations, treatment, prognosis, and prevention between proximal and distal GC to provide guidance and a basis for clinical diagnosis and treatment.
Monodopsis and Vischeria Genomes Shed New Light on the Biology of Eustigmatophyte Algae
Authors: Yang Hsiao-Pei , Wenzel Marius , Hauser Duncan A , Nelson Jessica M , Xu Xia , Eliáš Marek , Li Fay-Wei 
Published: October 2021

Abstract
Members of eustigmatophyte algae, especially Nannochloropsis and Microchloropsis, have been tapped for biofuel production owing to their exceptionally high lipid content. Although extensive genomic, transcriptomic, and synthetic biology toolkits have been made available for Nannochloropsis and Microchloropsis, very little is known about other eustigmatophytes. Here we present three near-chromosomal and gapless genome assemblies of Monodopsis strains C73 and C141 (60 Mb) and Vischeria strain C74 (106 Mb), which are the sister groups to Nannochloropsis and Microchloropsis in the order Eustigmatales. These genomes contain unusually high percentages of simple repeats, ranging from 12% to 21% of the total assembly size. Unlike Nannochloropsis and Microchloropsis, long interspersed nuclear element repeats are abundant in Monodopsis and Vischeria and might constitute the centromeric regions. We found that both mevalonate and nonmevalonate pathways for terpenoid biosynthesis are present in Monodopsis and Vischeria, which is different from Nannochloropsis and Microchloropsis that have only the latter. Our analysis further revealed extensive spliced leader trans-splicing in Monodopsis and Vischeria at 36–61% of genes. Altogether, the high-quality genomes of Monodopsis and Vischeria not only serve as the much-needed outgroups to advance Nannochloropsis and Microchloropsis research, but also shed new light on the biology and evolution of eustigmatophyte algae.
La tesis sobre la especificidad de la interpretación constitucionalThe specificity thesis of constitutional interpretation
Authors: Celis Vela Dúber Armando 
Published: October 2021

Abstract
The special nature of constitutional interpretation is a distinctive feature of contemporary constitutionalism. The justification for this thesis is usually presented in a dispersed and unsystematic way. The purpose of this paper is to reconstruct the arguments that promote the thesis of the special interpretation of the constitution and propose a theoretical model to determine its conceptual scope. In general terms, I argue that the constitution and the techniques of constitutional interpretation do not imply special operations to establish the meaning of the constitutional provisions. The doctrine of special interpretation is justified in constitutionalist conceptions of the law to generate attitudes favorable to the discretionary decisions adopted by the constitutional judge.
Association Between Immunogenicity and Reactogenicity: A Post Hoc Analysis of 2 Phase 3 Studies With the Adjuvanted Recombinant Zoster Vaccine
Authors: Callegaro Andrea , Burny Wivine , Hervé Caroline , Hyung Kim Joon , Levin Myron J , Zahaf Toufik , Cunningham Anthony L , Didierlaurent Arnaud M 
Published: October 2021

Abstract
A recurrent question is whether transient reactions to vaccines translate into better immune responses. Using clinical data from 2 large phase 3 studies of the recombinant zoster vaccine, we observed a small but statistically significant association between the intensity of a frequent side effect (pain) after vaccination and immune responses to vaccination. However, despite the statistical correlation, the impact on the immune response is so small, and the immune response in individuals without pain already sufficient, that pain cannot be a surrogate marker for an appropriate immune response. Reactogenicity cannot be used to predict immunity after vaccination.
PSII-2 Daily patterns for respiration rates and internal temperatures of heat stressed lactating sows with or without electronically controlled cooling pads
Authors: Graham Jason R , Galvin Michaiah J , Jansen Taylor L , Field Tyler C , Stwalley Robert M , Johnson Jay S , Schinckel Allan P 
Published: October 2021

Abstract
Lactating sows are especially sensitive to heat stress (HS) due to high metabolic demands resulting from milk output. Therefore, there is a need to develop effective cooling technologies that reduce the impact of HS on lactating sows. The study objective was to determine whether electronically controlled cooling pads (ECP) would allow sows to remain euthermic under HS conditions. Twelve multiparous (2.69 ± 0.85) lactating sows (265.4 ± 26.1 kg) and litters (11.4 ± 0.7 piglets/litter) were assigned to either a non-functional ECP (NECP; n = 6) or an ECP (n = 6), housed in farrowing crates, and tested over two repetitions. Sows were provided feed and water ad libitum and all sows were exposed to HS (28.27 ± 1.42°C nighttime to 35.14 ± 0.70°C daytime). Body temperature (TB), was measured hourly using vaginal implants, and respiration rate (RR) was measured in 30 min intervals from 0600 to 2100 hrs over 2 d representing mid- and late lactation. Mixed model periodic regression equations were fitted to the TB and RR to time. The final model for TB included the effect of replicate, random effect of sow, day of lactation as a covariate, single-phase sine and cosine variables for each treatment and 2-phase sine and cosine periodic regression variables. The final model for RR included the random effect of sow, effect of treatment, replicate, day of lactation, sleeping, and single-phase sine and cosine functions for each treatment. An interaction (P &lt; 0.05) between treatment and sine or cosine was observed for TB. For RR there was no interaction for treatment and cosine (P = 0.07), but an interaction (P &lt; 0.05; -9.94) was observed for sine. The significant treatment by wavelength interactions observed indicate a change in RR and TB patterns when sows are placed on ECPs.
PSXV-23 Effects of a nutritional packet composed of live yeast, vitamins, and electrolytes on the feeding behavior of intensively managed beef steers
Authors: Coello Kymberly D , Osorio Andrea M , Nardi Kaue Tonelli , Scaranto Silva Kaliu G , Raudales Angel A , Ciriaco Francine M , Sarturi Jhones Onorino , Henry Darren D 
Published: October 2021

Abstract
The effect of a nutritional-packet was evaluated on time spent ruminating, eating, and chewing (ruminating + eating) by intensively managed beef steers consuming a finishing diet during the final 65 d of feeding prior to slaughter. Furthermore, the influence of behavior on CH4 production was determined. Twenty-four Angus-crossbreed steers were distributed into pens with SmartFeed (C-Lock) technology in which they were treated with: control or nutritional-packet [0.29% DM basis; live yeast (8.7 Log CFU/g); Vitamin C (5.4 g/kg); Vitamin B1 (13.33 g/kg); NaCl (80 g/kg); KCl (80 g/kg)]. An ear tag containing multiple accelerometers (CowManager) was placed on steers, and such device continuously recorded rumination and eating time (min/day). Enteric CH4 emissions were measured for 15 d using the SF6 tracer technique. Feeding behavior data were analyzed as a randomized complete block design using the MIXED procedure of SAS. The model included the fixed effect of treatment and the random effect of block. Steer was considered experimental unit. The GLM procedure of SAS was used for regressing behavior and CH4 production data. There were no differences between treatments for time (within 24 h) spent ruminating, eating, or chewing (P ³ 0.197). When nutrient intake was taken into account, no differences (P ³ 0.139) were observed between treatments for time spent ruminating, eating, and chewing per kg of dry matter, organic matter, neutral detergent fiber intake, and acid detergent fiber intakes. The production of CH4 was not affected by time spent ruminating (0.93 g/min; R2 = 0.0003; P = 0.935), eating (3.34 g/min; R2 = 0.03; P = 0.409), or chewing (0.69 g/min; R2 = 0.006; P = 0.728). The feeding behavior of intensively managed beef steers during the final 65-d of feeding was not driven by the nutritional-packet supplied, neither was CH4 production associated with feeding behavior variables measured.
PSX-B-14 Properties of Kluyveromyces marxianus strains isolated from natural biotopes
Authors: Nikanova Daria A , Artemyeva Olga 
Published: October 2021

Abstract
Kluyveromyces marxianus yeast is often isolated from dairy products and used as probiotic agents in food. As a producer-yeast they must be genetically stable, have simple nutrient requirements, be effective in hostile conditions, grow on inexpensive nutrient media and be easy to store and distribute. Nineteen strains of K. marxianus isolated from natural biotopes were tested for their ability to grow on various carbon sources. For this, the YPD medium (1% yeast extract, 2% peptone, 2% dextrose, pH 5.5) was used with the addition of 20 g/L of one of the following carbon sources: glucose, sucrose, lactose, maltose, mannitol, arabinose, xylose, and galactose. The cultivation was carried out for 1–3 days. All isolates were also tested for their ability to grow in YPD broth at different temperatures (4, 27, 37, 40, 42, 46 and 48 °C) and pH (5; 3 and 2). K. marxianus strains showed widespread use of the substrate and high resistance to elevated incubation temperatures. All tested strains grew at 27 and 37 °C and one strain grew at 46 °C. None of the strains showed active growth at pH 2 and all cultures grew actively at pH 3. At the same time five strains showed growth at pH 5.0. All strains fermented galactose, 16 - xylose, 9 - glucose, 7 - lactose, 12 - maltose, 11 - arabinose, 5 - sucrose, 13 - mannitol. It should be noted that all strains in this study have showed fully restored growth after 2 hours’ incubation at pH 2. It indicates that they could survive when passing through the gastric passage into the intestine. These properties make the use of K. marxianus and its metabolites indispensable in agriculture as a feed additive. This work was supported by the Ministry of Science and Higher Education of Russia.
PSX-B-13 Effect of epidermal growth factor and prolactin on the quality of bovine oocytes aging in vitro
Authors: Shedova Ekaterina , Singina Galina , Lebedeva Irina Y , Lopukhov Aleksandr 
Published: October 2021

Abstract
The evaluation of factors responsible for the protection of the oocytes attained the metaphase-II stage from aging is importance for successful in vitro embryo reproduction. The aim of the present research was to study dose-dependent effects of epidermal growth factor (EGF) and prolactin (PRL) on the quality of bovine oocytes after their aging in vitro. Bovine cumulus-enclosed oocytes (CEOs) were matured in vitro for 20 h in TCM 199 containing 0.2 mM sodium pyruvate, 10% fetal calf serum (FCS), 10 μg/ml FSH and LH. At the end of in vitro maturation, oocytes were transferred to TCM 199 supplemented with 10% FCS (aging medium) and cultured for additional 24 h in the absence (Control) and in presence of EGF (10 and 50 ng/ml) and PRL (20 and 50 ng/ml). After prolonged culture oocytes were used for apoptosis detection (TUNEL staining, n=251) and the state of chromosomes evaluation (Tarkowski’s cytogenetic method, n=359). The data from 3–4 replicates were analyzed by ANOVA. At the end of prolonged culture (24 h) the rate of apoptotic oocytes in the Control group was 47.4±8.5%. EGF at concentration of 10 ng/ml and PRL at both doses decreased this rate to 15.0–22.1% (p &lt; 0.05). Furthermore, PRL (not EGF) reduced the frequency of abnormal chromosome modifications (decondensation, adherence, clumping) at concentrations of 20–50 ng/ml from 58.7±2.1% (Control) to 41.2±1.9 and 45.6±2.7% respectively (p &lt; 0.01). Thus, EGF and PRL is able to maintain the apoptosis resistance of bovine oocytes during their prolonged in vitro culture as well as PRL have the decelerating effect on abnormal modifications of M-II chromosomes. The research was supported by RFBR (17-29-08035) and the Ministry of Science and Higher Education of Russia.
PSX-B-11 Comparing effects of FGF2 and IGF1 on the development competence of parthenogenetic activated bovine oocytes maturing in vitro
Authors: Singina Galina 
Published: October 2021

Abstract
The oocyte quality acquired during in vitro maturation (IVM) are the main limitative factors affecting the embryo production. The aim of the present research was to study effects of fibroblast growth factor 2 (FGF2) and insulin-like growth factor 1 (IGF1) during IVM of bovine oocytes on their developmental potential after parthenogenetic activation. Bovine cumulus-oocyte complexes (COC; n = 1176) were cultured for 22h in either standard maturation medium (TCM-199 supplemented with 10% fetal calf serum (FCS), 0.2 mM sodium pyruvate, 10 μg/ml FSH and 10 μg/ml LH; Control) or maturation medium supplemented with different concentrations (5–160 ng/ml) of FGF2 and IGF1. After IVM, matured oocytes activated by sequential treatment with ionomycin followed by DMAP and cyclohexamide and then cultured up to the blastocyst stage. The obtained blastocysts were fixed, and the total cell number and the level of apoptosis were determined using DAPI and TUNEL staining. The data from 4 replicates (77–91 oocytes per treatment) were analyzed by ANOVA. Cleavage rates of activated oocytes did not differ between groups and ranged from 63.7 to 68.1%. The addition of 10, 20 and 40 ng/ml of FGF2 to the IVM medium led to an increase in the yield of blastocysts [from 19.6±1.8% (Control) to 35.2±3.4, 29.8±1.9 and 31.1±2.1%, respectively (P&lt;0.05)] and in the total cell number in embryos that developed to the blastocyst stage (P&lt;0.05). Meanwhile, the blastocyst yield and the total cell number in blastocysts in the IGF1-treated groups were similar to that in the control group. No effects of both growth factors on the proportion of apoptotic nuclei in blastocysts (5.3–7.1%) were observed. Thus, FGF2 (but not IGF1) are able to maintain competence for parthenogenetic development of bovine COC during their maturation invitro. Supported by RFBR (18-29-07089) and the Ministry of Science and Higher Education of Russia.
PSX-B-12 Сreation of a pichia pastoris strain: A producer of recombinant peptidase M9 from Aeromonas salmonicida
Authors: Minaev Mikhail , Nagel Alexey , Andreeva-Kovalevskaya Zhanna , Zakharova Marina , Khvostov Daniil 
Published: October 2021

Abstract
Proteases are produced by a wide variety of microorganisms and have a wide range of applications. Peptidase M9 from Aeromonas salmonicida is a 915 amino acid residue protein. Earlier, we cloned the 864 bp C end of the gene. (col2 gene) encoding the actual peptidase domain of 238 aa in the vector plasmid pPic9K. It was demonstrated that the recombinant Pichia pastoris strain, created on the basis of this plasmid, has a specific enzymatic activity against collagen. The aim of this work was to further optimize the expression of the col2 gene of the M9 peptidase domain from Aeromonas salmonicida in Pichia pastoris, isolation, purification, and characterization of the protein.First, the col2 gene was optimized based on the use of P. pastoris codons. Secondly, a study of collagenase activity with 4 different signaling sequences was carried out. It was shown that the strain for which the collagenase gene had the P. pastoris alpha peptide as a signal sequence was shown to have the maximum activity. One of the approaches leading to an increase in the yield of the target protein is the cloning of the gene encoding the chaperone proteins. We have created strains containing, in addition to the collagenase gene, also the pdi and hac chaperone genes. It was shown that the collagenase activity in the strain containing the pdi chaperone gene was 2–3 times higher than in the strain containing only the col2 gene. So, the Pichia pastoris strain was created - the producer of the recombinant peptidase M9 from Aeromonas salmonicida, the target protein was isolated and characterized.Research conducted as part of the state assignment of Gorbatov Federal Research Center for Food Systems of Russian Academy of Sciences 0437-2019-0001.