bluuebunny/crossref_metadata_2025_split huggingface dataset:
L-Arginine supplementation as mitochondrial therapy in diabetic cardiomyopathy
Authors: Fiordelisi Antonella , Cerasuolo Federica Andrea , Avvisato Roberta , Buonaiuto Antonietta , Maisto Marianna , Bianco Antonio , D’Argenio Valeria , Mone Pasquale , Perrino Cinzia , D’Apice Stefania , Paolillo Roberta , Pezone Antonio , Varzideh Fahimeh , Santulli Gaetano , Sorriento Daniela , Iaccarino Guido , Gambardella Jessica 
Published: December 2024

AbstractIn patients with type II diabetes, the development of diabetic cardiomyopathy (DC) is associated with a high risk of mortality. Left ventricular hypertrophy, diastolic dysfunction, and exercise intolerance are the first signs of DC. The underlying mechanisms are not fully elucidated, and there is an urgent need for specific biomarkers and molecular targets for early diagnosis and treatment. Mitochondrial alterations play a key role in the development of DC, and microRNAs regulating mitochondrial function are emerging as potential biomarkers of metabolic stress in DC. L-Arginine (Arg) supplementation has been shown to be an effective strategy for improving mitochondrial function and energetics, with a significant impact on physical performance. The aim of the current study was to evaluate the effects of Arg supplementation on cardiac mitochondrial function, DC development, and relative phenotypes including exercise intolerance. We used db/db mice as a model of type II diabetes, chronically treated with Arg (1 mg/kg/day) for 12 weeks. Arg-treated db/db mice showed preserved diastolic function and left ventricular morphology compared with untreated diabetic mice. Arg supplementation also improved exercise tolerance and the propensity to physical activity. Mitochondrial respiration was significantly increased in cardiomyocytes isolated from treated db/db mice, as well as in diabetic cardiomyocytes treated with Arg in vitro. The improvement of cardiac mitochondrial function in db/db + Arg mice was associated with an increase in PGC-1-alpha levels, mitochondrial biogenesis, recycling, and antioxidant capacity. Arg treatment prevented the accumulation of circulating and cardiac miR-143 in db/db mice, which is an index of metabolic stress and activation of mitochondrial damage mechanisms. In conclusion, Arg supplementation is effective in preventing the development of DC, preserving diastolic function and exercise tolerance by improving mitochondrial fitness and homeostasis. Additionally, miR-143 could potentially be employed to monitor cardiac metabolic stress and the effects of Arg treatment in diabetes.
Clinical importance of patient-reported outcome measures in severe asthma: results from U-BIOPRED
Authors: Meys Roy , Franssen Frits M.E. , Van ‘t Hul Alex J. , Bakke Per S. , Caruso Massimo , Dahlén Barbro , Fowler Stephen J. , Geiser Thomas , Howarth Peter H. , Horváth Ildikó , Krug Norbert , Behndig Annelie F. , Singer Florian , Musial Jacek , Shaw Dominick E. , Montuschi Paolo , Zee Anke H. Maitland-van der , Sterk Peter J. , Roberts Graham , Kermani Nazanin Z. , Incalzi Raffaele A. , Louis Renaud , Andersson Lars I. , Wagers Scott S. , Dahlén Sven-Erik , Chung Kian Fan , Adcock Ian M. , Spruit Martijn A. ,   on behalf of the U-BIOPRED Study Group
Published: December 2024

Abstract
Rationale
Knowledge about the clinical importance of patient-reported outcome measures (PROMs) in severe asthma is limited.

Objectives
To assess whether and to what extent asthma exacerbations affect changes in PROMS over time and asthma-specific PROMs can predict exacerbations in adult patients with severe asthma in usual care.

Methods
Data of 421 patients with severe asthma (62% female; mean age 51.9 ± 13.4 years; mean FEV1 67.5 ± 21.3%pred) from the U-BIOPRED cohort were analyzed. The included PROMs were: Asthma Control Questionnaire (ACQ5); Asthma Quality of Life Questionnaire (AQLQ); Hospital Anxiety and Depression scale (HADS); Epworth Sleepiness Scale (ESS); Medication Adherence Report Scale (MARS); Sino-Nasal Outcomes Test (SNOT20). Participants were assessed at baseline and after 12–18 months of usual care.

Results
PROMs showed very weak to weak correlations with clinical characteristics such as age, body mass index, FEV1, FeNO and eosinophilic cell count. Patients presenting no exacerbations during follow-up showed a statistically significant improvement in all PROMs (except for MARS), whereas individuals experiencing > 2 exacerbations showed a deterioration. Baseline ACQ5 was a predictor of exacerbations with an AUC of 0.590 (95%CI 0.514–0.666).

Conclusions
The association of PROMs with clinical measures was poor in severe asthmatics. Moreover, PROMs were prone to changes in usual care, with exacerbations playing a key role. PROMs need to be systematically evaluated in severe asthma to improve clinical care based on specific patient’s needs.
Is there a comparable Mp-MRI for incidental prostate uptake on 18 F-FDG PET/CT?
Authors: Şam Özdemir Merve , Kaya Nurullah , Savun Metin , Keskin Emin Taha , Yüzkan Sabahattin , Arslan Fatma Zeynep , Budak Burcu , Omak Özgür , Yardımcı Aytül Hande , Özdemir Harun 
Published: December 2024

Abstract
Purpose
Although 18 F-FDG-PET/CT is helpful in defining many types of cancer, localized prostate cancer should not be treated with this technique. This study describes the use of multi-parametric MRI (mpMRI) to characterize incidental 18 F-FDG uptake in the prostate.

Methods and Materials
While 18 F-FDG-PET/CT is useful for characterizing a variety of cancers, it is not advised for prostate cancer that is localized. This work investigates the use of mpMRI to describe incidental 18 F-FDG uptake in the prostate.mpMRI included T2-weighted (T2W), dynamic contrast enhancement (DCE), and apparent diffusion coefficient (ADC) sequences. Patients were classified according to PI-RADS (Prostate Imaging Reporting and Data System) version 2.1 by an experienced uroradiologist, and 18 F-FDG-PET was evaluated to determine whether the area of involvement on CT had a counterpart in mpMRI. A biopsy was performed on 30 of the 92 patients. These patients’ maximum standardized uptake values (SUVmax) 6 < and ≥ 6, PS(PSA) density 0.15 < and ≥ 0.15, PSA level, uptake pattern (focal involvement/diffuse involvement), and PI-RADS scores were compared. P < .05 was considered statistically significant. Logistic regression was used to analyze PI-RADS score groups age, PSA, PSA density and SUVmax.

Results
In the study, 92 patients with incidental 18 F-FDG-PET/CT prostate uptake were examined. Median age was 66, PSA median was 3.6 ng/ml (range: 0-3198 ng/ml). Notably, in 70.6% of cases, PET/CT uptake didn’t correlate with mp-MRI findings. Among PI-RADS 3-4-5 patients (29.3%), there was a correlation. Biopsies in 30 patients revealed 43.3% benign, 56.7% malignant. Significant differences between benign and malignant cases were observed in PSA density, PI-RADS scores, and PSA levels (p < .05), while SUVmax and uptake pattern were not significant. In multivariate logistic regression analysis, PI-RADS score groups were found to be independent risk factors for predicting malignancy.

Conclusions
Our study showed that incidental 18 F-FDG-PET/CT prostate uptake was detected and that high PSA density values, PI-RADS scores, and PSA values, such as in routine patients, and not PET-CT findings such as SUVmax and uptake pattern, were more predictive of malignancy.
Associations between ADC histogram analysis values and tumor-micro milieu in uterine cervical cancer
Authors: Surov Alexey , Borggrefe Jan , Höhn Anne-Kathrin , Meyer Hans-Jonas 
Published: December 2024

Abstract
Background
The complex interactions of the tumor micromilieu may be reflected by diffusion-weighted imaging (DWI) derived from the magnetic resonance imaging (MRI). The present study investigated the association between apparent diffusion coefficient (ADC) values and histopathologic features in uterine cervical cancer.

Methods
In this retrospective study, prebiopsy MRI was used to analyze histogram ADC-parameters. The biopsy specimens were stained for Ki-67, E-cadherin, vimentin and tumor-infiltrating lymphocytes (TIL, all CD45 positive cells). Tumor-stroma ratio (TSR) was calculated on routine H&E specimens. Spearman’s correlation analysis and receiver-operating characteristics curves were used as statistical analyses.

Results
The patient sample comprised 70 female patients (age range 32–79 years; mean age 55.4 years) with squamous cell cervical carcinoma. The interreader agreement was high ranging from intraclass coefficient (ICC) = 0.71 for entropy to ICC = 0.96 for ADCmedian. Several ADC-histogram parameters correlated strongly with the TSR. The highest correlation coefficient achieved p10 (r = -0.81, p < 0.0001). ADCmean can predict tumors with high TSR, AUC: 0.91, sensitivity: 0.91 (95% CI 0.77;0.96), specificity: 0.91 (95% CI 0.78;0.97). Several ADC-histogram parameters correlated slightly with the proliferation index Ki-67. No associations were found with TIL, E-Cadherin and vimentin. In well and moderately differentiated cancers, ADC histogram values showed stronger correlations with Ki-67 and TSR than in poorly differentiated tumors.

Conclusion
ADC values are strongly associated with tumor-stroma ratio. The ADC mean can be used to predict tumors with high TSR. Associations between histopathology and ADC values depend on tumor differentiation. ADC values show only weak associations with Ki-67 and none with TIL, vimentin and E-cadherin.
Hypermagnesemia caused by fecal-mass obstruction in stenotic rectal cancer following preoperative administration of magnesium citrate
Authors: Sato Yuri , Hashiba Eiji , Yamazaki Yuuma , Kato Koudai , Kinoshita Hirotaka , Noguchi Satoko , Kudo Tomoyuki , Hirota Kazuyoshi 
Published: December 2024

Abstract
Background
Hypermagnesemia is a rare complication, leading to fatal cardiovascular and respiratory conditions. We present severe hypermagnesemia developed in a patient with a rectal stenosis after pretreatment with oral magnesium citrate for rectosigmoid surgery.

Case presentation
A 78-year-old woman demonstrated consciousness disturbance, muscle weakness, and respiratory depression requiring tracheal intubation after preparation with oral magnesium for rectosigmoid surgery. Endoscopic examination showed a rectal obstruction due to fecal impaction. General condition improved after emergency Hartmann’s surgery. Blood test revealed a remarkable increase of serum magnesium level to 17.5 mg/dL when the general condition deteriorated, which would have been responsible for her symptoms. She was discharged from the ICU after extubation on the third postoperative day with a normal magnesium level.

Conclusions
Pretreatment with large doses of oral magnesium-containing bowel cleanser may cause severe hypermagnesemia in patients with colorectal stenosis.
Population-based cross-sectional analysis of cannabis use among Kentucky adults, 2020–21
Authors: Shafer Sydney , Kennedy Gunnar , Christian W. Jay 
Published: December 2024

Abstract
Purpose
We conducted this study to assess cannabis use rates in the state of Kentucky relative to socioeconomic, demographic, and geographic factors, as well as reasons for use and modes of use, before the legal medical marijuana market commences in 2025.

Methods
We pooled Kentucky Behavioral Risk Factor Surveillance System (BRFSS) data for 2020–2021 and used weighted responses for all analyses. We estimated current cannabis use (at least once in the past 30 days), and heavy use (at least 20 of the past 30 days) prevalence rates for Appalachian, Delta, and Central geographic regions of Kentucky. We tabulated descriptive statistics and used multivariable logistic regression to identify characteristics of individuals who used cannabis.

Results
The prevalence of cannabis use was lower in Kentucky (10%) than nationally (about 13%). Of those who used cannabis, 42% used it daily or near daily. Those who were male, ages 18–34, never married, black, less than HS education, lower household income, and lived in the Central region were more likely to use cannabis. Among those who used cannabis, mode of use varied somewhat among age groups, education levels, income groups, and marital status, but smoking was most common—78% overall. About 33% reported using cannabis for recreation alone, 24% for medical reasons alone, and 43% for both reasons.

Conclusion
Despite the illegal status of cannabis in Kentucky, its use is common across population sub-groups. A large proportion of Kentuckians using cannabis do so daily or near daily, and most for a medical purpose. Smoking, however, remains the most common mode of use.
Evaluation of the metabolic activity, angiogenic impacts, and GSK-3β signaling of the synthetic cannabinoid MMB-2201 on human cerebral microvascular endothelial cells
Authors: AL-Eitan Laith Naser , Alahmad Saif Zuhair , Ajeen Sufyan Ali , Altawil Ahmad Younis , Khair Iliya Yacoub , Kharmah Hana Salah Abu , Alghamdi Mansour Abdullah 
Published: December 2024

AbstractAngiogenesis is an intrinsic physiological process involving the formation of new capillaries from existing ones. Synthetic cannabinoids refer to a class of human-made chemicals that are primarily designed to mimic the effects of delta-9-tetrahydrocannabinol, the primary psychoactive compound in cannabis. Studies investigating the association between synthetic cannabinoids and cellular reactions are limited, and the available scientific evidence is insufficient. Consequently, the primary goal was to examine the effects of the synthetic cannabinoid MDMB-2201 on brain angiogenesis in vitro to provide a comprehensive analysis of MMB-2201’s potential therapeutic or adverse effects on vascular development and related health conditions. Human Cerebral Microvascular Endothelial Cells (HBEC-5i) were incubated with MMB-2201, and their metabolic activity, migration rate, and tubular structure formation were examined. Expression levels of several angiogenesis-related proteins such as vascular endothelial growth factor (VEGF), Angiopoietin-1 (ANG-1), and Angiopoietin-2 (ANG-2) were assessed using western blot, ELISA, and real-time PCR. Furthermore, the phosphorylation of glycogen synthase kinase 3 beta (GSK-3β) at Ser9 induced by MMB-2201 was evaluated. HBEC-5i cells showed a significant increase in metabolic rate, enhanced migration, and sprouting of brain endothelial cells. Moreover, there was a noticeable increase in the mRNA and protein levels of VEGF, ANG-1, and ANG-2, as well as in the phosphorylation rate of GSK-3β at Ser9. This study paves the way for a novel pharmacological approach to addressing various angiogenesis-related diseases by targeting cannabinoid receptor type-1. Further exploration using different antagonists or agonists of cannabinoid receptors, depending on the specific characteristics of the disorders, may be necessary.
Socioscientific issues: promoting science teachers’ pedagogy on social justice
Authors: Macalalag Augusto Z. , Kaufmann Alan , Van Meter Benjamin , Ricketts Aden , Liao Erica , Ialacci Gabrielle 
Published: December 2024

AbstractSocioscientific issues (SSI) are problems involving the deliberate use of scientific topics that require students to engage in dialogue, discussion, and debate. The purpose of this project is to utilize issues that are personally meaningful and engaging to students, require the use of evidence-based reasoning, and provide a context for scientific information. Social justice is the pursuit of equity and fairness in society by ensuring that all individuals have opportunities to challenge and address inequalities and injustices to create a more just and equitable society for all (Killen et al. Human Development 65:257–269, 2021). By connecting science, technology, engineering, and mathematics (STEM) concepts to personally meaningful contexts, SSI can empower students to consider how STEM-based issues reflect moral principles and elements of virtue in their own lives and the world around them (Zeidler et al. Science Education 89:357–377, 2005). We employed a qualitative research design to answer the following questions: (1) In what ways, if any, did teachers help students grow their knowledge and practices on social justice through socioscientific issues? (2) In teachers’ perceptions, what components of SSI did students learn and what are their challenges? (3) In teachers’ perceptions, what are students’ stances on social justice? After completing the first year and second-year professional development programs, grades 6–12 STEM teachers were asked to complete a reflection on classroom artifacts. Teachers were asked to select student artifacts (e.g. assignments, projects, essays, videos, etc.) that they thought exemplified the students’ learning of SSI and stance on social justice. Based on 21 teacher-submitted examples of exemplar student work, we saw the following example pedagogies to engage their students on social justice: (a) making connections to real-world experiences, (b) developing a community project, (c) examining social injustice, and (d) developing an agency to influence/make changes. According to teachers, the most challenging SSI for students was elucidating their own position/solution, closely followed by employing reflective scientific skepticism. Moreover, the students exemplified reflexivity, metacognition, authentic activity, and dialogic conversation. Using SSI in classrooms allows students to tackle real-world problems, blending science and societal concerns. This approach boosts understanding of scientific concepts and their relevance to society. Identifying methods like real-world connections and examining social injustice helps integrate social justice themes into science education through SSI. Overall, SSI promotes interdisciplinary learning, critical thinking, and informed decision-making, enriching science education socially. This study highlights the value of integrating SSI in science education to engage students with social justice.
Big data analytics in healthcare: data sources, tools, challenges, and opportunities
Authors: Badawy Mohammed , Ramadan Nagy , Hefny Hesham Ahmed 
Published: December 2024

AbstractThe increasing number of patients with chronic diseases and the concentration of medical resources have a substantial economic impact, leading to hospital visits, hospital readmissions, and additional healthcare expenses. Healthcare providers must now adopt big data strategies to keep up with the ever-increasing data deluge and enhance patient care. Therefore, this study aims to provide an overview of big data’s role in healthcare prediction by discussing its data sources, analytical techniques, and challenges. Also, it proposes a conceptual framework to be adopted in real-time big healthcare data analytics. In addition, this paper answers the following questions. First, what are the most popular Big Data sources that can be utilized in healthcare prediction? Second, how can Big Data sources and Big Data processing frameworks be integrated to enhance healthcare prediction accuracy? The studies discussed in this paper have been presented in popular scientific research databases, such as IEEE, Springer, and Elsevier. Machine learning, deep learning, and healthcare are some terms used to search for these studies. Hence, a review of published papers utilizing machine learning and deep learning methods for the purposes of diagnosing, detecting, predicting, and monitoring conditions pertaining to healthcare has been conducted. The conducted review identified the key challenges, research directions, and recommendations for real-time healthcare prediction using machine learning technologies. In addition, the proposed conceptual framework can serve as a general methodology to be adopted by other researchers in the healthcare domain.
Blockchain-based smart grid power trading technology
Authors: Su Kai , Yu Yun , Zhang Jianzhong 
Published: December 2024

AbstractTo improve the security and efficiency of smart grid power trading, this study designs a smart grid trading system based on blockchain technology. The transaction module of the system composed of smart contracts and order-matching algorithms is proposed. The credit consensus mechanism of the system adopts a designed credit-based hybrid consensus mechanism. The experimental results showed that under this system, the successful transaction prices of consumers bidding were between the grid price and the recycling price, making bidding more reasonable. The highest power utilization rate was 0.84, the lowest was 0.67, and the transaction throughput exceeded 350TPS. The highest CPU usage was 0.74, the lowest was 0.5, and the highest memory usage was only 0.44. Compared with other systems, the order matching of this system was more reasonable and its performance was better. Furthermore, the system has been subjected to rigorous security testing and is reliable, thereby providing a secure and efficient trading platform for smart grid electricity trading.
Cardiac myxoma with high standardized uptake value of FDG-PET-CT in the right ventricular outflow tract
Authors: Uehara Hiroo , Ohba Kenta , Ono Makoto , Imazuru Tomohiro , Shimokawa Tomoki 
Published: December 2024

Abstract
Background
Cardiac myxoma rarely occurs in the right ventricle, and as is a benign disease, it rarely shows high positivity on 18F fluorodeoxyglucose (FDG) positron emission tomography/computed tomography (PET/CT).
Case presentation.
We present herein the case of a 77-year-old woman who was found to have a heart murmur during a routine health checkup. Further examination revealed a 27-mm tumor in the right ventricular outflow tract (RVOT) and moderate aortic valve stenosis. Additionally, during her preoperative evaluation, she was diagnosed with a 10-mm tumor in the right breast, prompting her referral to our hospital for further evaluation and treatment. Contrast-enhanced CT and magnetic resonance imaging of the chest did not show signs strongly suggestive of malignancy, nor did echocardiography. However, FDG-PET/CT showed an abnormally high standardized uptake value (SUV) max of 9.91. Based on these findings, we decided the best treatment course was tumor resection of the RVOT and aortic valve replacement. Our intraoperative examination confirmed a tumor inferior to the pulmonary valve; therefore, we resected three branches of the septal artery feeding the tumor, including part of the right ventricular free wall. A rapid pathological examination indicated a benign tumor, and the patient’s final diagnosis was a cardiac myxoma. The postoperative course was uneventful, and to date, workup including CT scans during follow-up has shown no obvious recurrence.

Conclusion
This case highlights the challenges and importance of accurate imaging diagnoses in cardiac tumors. The patient underwent a successful surgical resection of the cardiac myxoma, emphasizing the need for careful postoperative follow-up.