bluuebunny/crossref_metadata_2025_split huggingface dataset:
Middle power hedging in the era of security/economic disconnect: Australia, Japan, and the ‘Special Strategic Partnership’
Authors: Wilkins Thomas 
Published: September 2021

AbstractDeeping superpower rivalry between the United States and China has created acute strategic dilemmas for secondary powers in the Indo-Pacific such as Australia and Japan. This predicament is exacerbated by their divergent security and economic interests which cut across the superpower divide; a condition dubbed a ‘security/economic disconnect’. These two intimately related dynamics preclude clear-cut implementation of conventional balancing/bandwagoning alignment choices and have led to mixed hedging strategies to cope with this situation. To address these issues, the article presents a refinement of the hedging concept in International Relations (IR) that emphasizes its multi-dimensional nature, within a broader interpretation of alignment itself. It applies this to the case of the Australia and Japan with reference to their Strategic Partnership, which is both emblematic of hedging responses to systemic uncertainty, and an institutional mechanism through which to operationalize joint hedging policies. This provides insights into how middle power strategic partnerships are managing strategic risks across the security, economic, and other, domains.
Spanish adaptation and validation of the User Version of the Mobile Application Rating Scale (uMARS)
Authors: Martin-Payo Ruben , Carrasco-Santos Sergio , Cuesta Marcelino , Stoyan Stoyan , Gonzalez-Mendez Xana , Fernandez-Alvarez María del Mar 
Published: September 2021

Abstract

Objective
While the professional version of the Mobile App Rating Scale (MARS) has already been translated, and validated into the Spanish language, its user-centered counterpart has not yet been adapted. Furthermore, no other similar tools exist in the Spanish language. The aim of this paper is to adapt and validate User Version of the MARS (uMARS) into the Spanish language.

Materials and Methods
Cross-cultural adaptation, translation, and metric evaluation. The internal consistency and test-retest reliability of the Spanish version of the uMARS were evaluated using the RadarCovid app. Two hundred and sixteen participants rated the app using the translated scale. The app was then rated again 2 weeks later by 21 of these participants to measure test-retest reliability.

Results
No major differences were observed between the uMARS original and the Spanish version. Discrimination indices (item-scale correlation) obtained appropriate results for both raters. The Spanish uMARS presented with excellent internal consistency, α = .89 and .67 for objective and subjective quality, respectively, and temporal stability (r &gt; 0.82 for all items and subscales).

Discussion
The Spanish uMARS is a useful tool for health professionals to recommend high-quality mobile apps to their patients based on the user’s perspective and for researchers and app developers to use end-user feedback and evaluation, to help them identify highly appraised and valued components, as well as areas for further development, to continue ensuring the increasing quality and prominence of the area of mHealth.

Conclusion
uMARS Spanish version is an instrument with adequate metric properties to assess the quality of health apps from the user perspective.
Establishment of a Chemiluminescence Immunoassay Combined with Immunomagnetic Beads for Rapid Analysis of Ochratoxin A
Authors: Zhang Bo , Liu Wenting , Liu Zhenjiang , Fu Xiaoling , Du Daolin 
Published: September 2021

Abstract

Background
Ochratoxin A (OTA) is a harmful mycotoxin and secondary metabolite produced by several species of Aspergillus and Penicillium. It is of vital importance to establish a sensitive method to detect OTA in various grains.

Objective
A novel, highly sensitive chemiluminescence immunoassay was developed to determine OTA in wheat, corn, and poultry feed.

Methods
The immunomagnetic beads were used as solid phase carriers and separators in the competitive chemiluminescence method. Anti-OTA monoclonal antibodies coated on the magnetic beads were applied as the capturing antibodies, in which OTA would compete with OTA-alkaline phosphatase in binding with OTA antibodies.

Results
The proposed method exhibited good linearity (R2 ≥ 0.999), adequate OTA recovery rate (83.60–102.50%), and good repeatability. The sensitivity of the proposed method was 2.05 pg/mL. In addition, the method was highly selective and showed no cross-reactions with other mycotoxins (e.g., AFB1, DON, ZEN). The developed method was applied in the analysis of wheat, corn, and two animal feeds successfully. The results obtained by the chemiluminescence method showed a high correlation with those obtained by the HPLC method (correlation coefficient 0.9958).

Conclusion
This strategy shows great potential for other toxic and harmful mycotoxin detection.

Highlights
A novel highly rapid and sensitive chemiluminescence immunoassay was established for quantitation of OTA.
Validation of Modifications to the Soleris®E. coli Method for Detection and Threshold Determination of Escherichia coli in Select Foods: Level 3 Modification to AOAC Performance Tested MethodSM 101101
Authors: Beck Nancy , Le Quynh-Nhi , Mozola Mark , Roman Brooke , Montei Carolyn , Zhang Lei , Biswas Preetha , Donofrio Robert 
Published: September 2021

Abstract

Background
Soleris®E. coli is an automated, growth-based method for detection and semi-quantitative determination of Escherichia coli in foods. The method can be used in dilute-to-specification (threshold) or presence/absence modes.

Objective
The objective of the study was to validate four modifications to the method: (1) a change in the vial detection window plug composition from agar to agarose to improve plug consistency and robustness, (2) a change in pre-enrichment incubation time for presence/absence testing from 6 h to 18–24 h, (3) a change in vial incubation temperature from 44.5 to 43.5°C, and (4) incorporation of a simple direct-from-vial confirmation test as an alternative to traditional procedures.

Methods
Elements of the study included inclusivity/exclusivity testing, matrix testing in comparison to the ISO 7251:2005 reference method, reagent stability/lot-to-lot consistency testing, and an independent laboratory study.

Results
In inclusivity testing, all 55 Escherichia coli strains tested produced positive results. In exclusivity testing, 30 of 31 strains of other bacterial species produced negative results, the sole exception being a strain of Enterobacter cloacae. In internal and independent laboratory matrix testing of mozzarella cheese, condensed milk, pasteurized liquid egg, and frozen green beans, results showed no significant differences in performance of the Soleris and reference methods with two exceptions, one in which the Soleris method produced more positive results, and one in which the reference method produced more positive results.

Conclusion
Performance characteristics of the modified Soleris E. coli method are consistent with those of the original validated method.

Highlights
The Soleris E. coli method offers improvements in ease of use and reagent robustness.
Qualitative LC–Q-TOF Analysis of Umbilical Cord Tissue via Data-Dependent Acquisition as an Indicator of In Utero Exposure to Toxic Adulterating Substances
Authors: Nelson Brandon N , Strathmann Frederick G , Browne Thom , Cervantes Abigail , Logan Barry K 
Published: September 2021

Abstract
Toxic adulterants are drug or chemical agents used to add bulk volume to traditional drugs of abuse such as cocaine and heroin. These cutting agents include levamisole, metamizole, noxiptillin, phenacetin and xylazine as well as common legal drugs such as acetaminophen, caffeine, diphenhydramine, lidocaine, quinine, quetiapine and tramadol. Because they possess pharmacological activity they result in exposure of the user, but also in the case of pregnant women, the developing fetus, to potential drug toxicity. We describe the development, validation and implementation of a rapid (48 second sample-to-sample) test based on a qualitative data-dependent liquid chromatography–quadrupole time of flight mass spectrometry method for the analysis of toxic adulterating substances in umbilical cord tissue (UCT) samples. The method provides a means of studying potential in utero exposure to these agents. Library spectra comparison at three different collision energies was used in conjunction with retention time and accurate mass to identify these substances in UCT. Analytically based reporting limits were established to determine positivity rates of adulterants in UCT utilizing a standard addition approach. The method was applied to authentic cocaine and opioid positive UCT to screen for toxic adulterants. There were a total of 82 potential adulterant positives found in a 30-sample cohort of authentic UCT samples, with an average of 2.7 substances per case. Lidocaine was the predominant finding followed by caffeine, and diphenhydramine all of which could result from non-illicit drug exposure, however, there were positives for levamisole, phenacetin, noxiptillin and xylazine none of which are approved in the United States for human therapeutic use. This initial set of data established a preliminary positivity rate of potentially toxic adulterants in UCT samples positive for cocaine or opioid use.
Streptomyces clavuligerus: The Omics Era
Authors: Liras Paloma , Martín Juan F 
Published: September 2021

Abstract
The Streptomyces clavuligerus genome consists in a linear chromosome of about 6.7 Mb and four plasmids (pSCL1 to pSCL4), the latter one of 1.8 Mb. Deletion of pSCL4, results in viable mutants with high instability in the chromosome arms, which may lead to chromosome circularisation. Transcriptomic and proteomic studies comparing different mutants with the wild-type strain improved our knowledge on the biosynthesis and regulation of clavulanic acid, cephamycin C and holomycin. Additional knowledge has been obtained on the SARP-type CcaR activator and the network of connections with other regulators (Brp, AreB, AdpA, BldG, RelA) controlling ccaR expression. The transcriptional pattern of the cephamycin and clavulanic acid clusters is supported by the binding of CcaR to different promoters and confirmed that ClaR is a CcaR-dependent activator that controls the late steps of clavulanic biosynthesis. Metabolomic studies allowed the detection of new metabolites produced by S. clavuligerus such as naringenin, desferroxamines, several N-acyl tunicamycins, the terpenes carveol and cuminyl alcohol or bafilomycin J. Heterologous expression of S. clavuligerus terpene synthases resulted in the formation of no less than 15 different terpenes, although none of them was detected in S. clavuligerus culture broth. In summary, application of the Omic tools results in a better understanding of the molecular biology of S. clavuligerus, that allows the use of this strain as an industrial actinobacterial platform and helps to improve CA production.
Multi-generational Effects of Different Resistant Wheat Varieties on Fitness of Sitobion avenae (Hemiptera: Aphididae)
Authors: Hu Xiang-Shun , Luo Chen , Li Guang-Kuo , Zhang Zhan-Feng , Wang Chun-Ping , Hu Zu-Qing , Zhao Hui-Yan , Liu Tong-Xian 
Published: September 2021

Abstract
Crop resistance plays a role in preventing aphid damage, benefiting food production industries, but its effects are limited due to aphid adaptation and phenotypic plasticity. Therefore, furthering understanding of aphid–crop interactions will improve our ability to protect crops from aphids. To determine how aphids adapt to resistant varieties of wheat, Triticum aestivum L. over time, we performed a laboratory experiment to assess the multi-generational effects of three wheat varieties, Batis, Ww2730, and Xiaoyan22, with different resistance levels on the fitness of Sitobion avenae (Fab.) (Hemiptera: Aphididae). The results showed that Ww2730 and Xiaoyan22 were more resistant than Batis to S. avenae, regardless of whether the aphids were newly introduced or had been acclimated before being introduced to the three wheat varieties. However, the effect of resistance on aphid life-history traits was time dependent. Aphid weigh gain increased and they development faster of the acclimated generation compared to the newly introduced generation on all three varieties. And the fecundity on the three varieties and net reproduction rates on Batis and Xiaoyan22 significantly decreased. Aphid fitness in terms of individual life-history parameters improved, whereas aphid fitness in terms of reproductive decreased, and a convergence effect, the difference gaps and standard errors of all life-history traits among the three acclimated populations had narrowed and were less than those in the three first-generation populations, was observed during the 3-mo experimental period. We suggested that S. avenae could rapidly respond to wheat resistance through life-history plasticity.
Differences in baseline risk estimated by physicians and patients with early breast cancer
Authors: Mori Makiko , Yoshimura Akiyo , Sawaki Masataka , Hattori Masaya , Kotani Haruru , Adachi Yayoi , Iwase Madoka , Kataoka Ayumi , Sugino Kayoko , Horisawa Nanae , Ozaki Yuri , Iwata Hiroji , Onishi Sakura , Gondo Naomi , Terada Mitsuo 
Published: September 2021

Abstract

Background
Physicians recommend adjuvant therapy to patients based on baseline risk. A common recognition for baseline risk between patients and physicians is critical for successful adjuvant therapy. We prospectively investigated the differences in estimated baseline risk between physicians and patients with early breast cancer.

Methods
This analysis was performed at a single institution in Japan. Early breast cancer patients over 18 years old were enrolled after surgery. After explaining the pathological results, physicians asked each patient about an estimated baseline risk. Differences in estimated baseline risk were defined as the baseline risk estimated by patients minus the baseline risk estimated by physicians. The primary endpoint was that the number of patients who estimate baseline risk higher than physicians was higher than those who estimate a lower baseline risk. The secondary endpoints were differences in estimated baseline risk by stage, subtype and the influence of patient factors to differences in estimated baseline risk.

Results
From July 2017 to December 2018, 262 patients were enrolled. Among the 262 patients, 190 estimated a higher baseline risk than physicians, 53 estimated a lower baseline risk and 19 estimated the same. Overall, patients estimated a significantly higher baseline risk than physicians (P &lt; 0.001). Differences in estimated baseline risk was significantly smaller in patients who knew the term ‘baseline risk’ than patients who did not (P = 0.0037). Differences in estimated baseline risk were also significantly smaller in patients with stage II breast cancer than patients with stage I (P = 0.0239). However, there were no statistically significant differences of differences in estimated baseline risk according to other factors.

Conclusions
Patients with early breast cancer estimated a significantly higher baseline risk than physicians. Physicians should accurately explain baseline risk to patients for shared decision making.
Mule deer do more with less: comparing their nutritional requirements and tolerances with white-tailed deer
Authors: Staudenmaier Anna R , Shipley Lisa A , Camp Meghan J , Forbey Jennifer S , Hagerman Ann E , Brandt Abigail E , Thornton Daniel H 
Published: September 2021

Abstract
Congeneric species often share ecological niche space resulting in competitive interactions that either limit co-occurrence or lead to niche partitioning. Differences in fundamental nutritional niches mediated through character displacement or isolation during evolution are potential mechanisms that could explain overlapping distribution patterns of congenerics. We directly compared nutritional requirements and tolerances that influence the fundamental niche of mule (Odocoileus hemionus) and white-tailed deer (O. virginianus), which occur in allopatry and sympatry in similar realized ecological niches across their ranges in North America. Digestible energy and protein requirements and tolerances for plant fiber and plant secondary metabolites (PSMs) of both deer species were quantified using in vivo digestion and intake tolerance trials with six diets ranging in content of fiber, protein, and PSMs using tractable deer raised under identical conditions in captivity. We found that compared with white-tailed deer, mule deer required 54% less digestible protein and 21% less digestible energy intake per day to maintain body mass and nitrogen balance. In addition, they had higher fiber, energy, and dry matter digestibility and produced glucuronic acid (a byproduct of PSM detoxification) at a slower rate when consuming the monoterpene α-pinene. The mule deers’ enhanced physiological abilities to cope with low-quality, chemically defended forages relative to white-tailed deer might minimize potential competitive interactions in shared landscapes and provide a modest advantage to mule deer in habitats dominated by low-quality forages.
Population survey of small rodents on islands located inside a region of endemism for hantavirus pulmonary syndrome
Authors: Maroli Malena , Burgos Eliana Florencia , Piña Carlos Ignacio , Gómez Villafañe Isabel Elisa 
Published: September 2021

Abstract
Ecological studies of rodent species, especially as reservoirs of zoonoses, can identify spatiotemporal conditions associated with irruptions of abundances, as well as predict areas and times with higher risk of disease transmission. The aims of this research were to describe and identify (i) the rodent community composition; (ii) their population structure and breeding season; (iii) temporal and spatial variations in their population abundance; and (iv) the environmental factors associated with these variations on islands of upper Paraná River Delta, a zone of endemism for hantavirus pulmonary syndrome (HPS) in Argentina (Entre Ríos and Santa Fe provinces). We carried out seasonal surveys over 3 years (2014–2017) with live capture traps on seven islands (natural protected areas and under livestock grazing). Three hundred seventy-seven sigmodontine rodents of seven species were captured. While the maximum richness was seven, only four species coexisted at most on an island at the same time. Although changes in reproduction were detected throughout the year, seasonality, land use, and vegetation structure did not explain changes in abundance of rodents. Rodent abundances were affected mainly by flooding-related factors and meteorological conditions (rainfall and temperatures). The recovery of rodent populations after river flooding was species-specific and heterogeneous. The abundance of Oxymycterus rufus and Akodon azarae was affected by El Niño Southern Oscillation (ENSO); A. azarae did not recover after the flood at least during the study period. After the flood, populations of Oligoryzomys flavescens, the reservoir of HPS, also declined; however, this species’ populations were the first to recover its numbers, becoming dominant in the post-flood rodent community.
Dietary flexibility and high predator efficacy facilitate coexistence in a novel predator interaction
Authors: Moseby Katherine E , Jensen Melissa A , Tatler Jack 
Published: September 2021

Abstract
Predator diet can be influenced by competition and intraguild predation, leading to resource partitioning and/or avoidance. For sympatric, endemic predators, these processes form as predator species coevolve, facilitating coexistence. However, when novel predator interactions occur, significant dietary overlap could create acute levels of competition leading to intraguild predation and population extinction, or accelerated changes in diet and/or spatial and temporal avoidance. We measured diet, intraguild predation, and spatial and temporal overlap in two predator species in a novel predator interaction: the western quoll (Dasyurus geoffroii), a small, native carnivore reintroduced to semi-arid Australia, and the domestic cat (Felis catus), a larger introduced carnivore already resident at the release site. Both species exhibited high dietary overlap and fed on mammals, reptiles, birds, and invertebrates. Cats included quolls in their diet. Quoll diet was broader (including carrion, bats, and plant material) and flexible, changing significantly with age, sex, and season. Introduced rabbit was the most common prey item recorded for both species (frequency of occurrence = 40–50%). However, quolls consumed rabbits in relation to their availability while rabbit consumption in cats was unrelated to availability suggesting a stronger dependence on rabbit prey. Quoll diet did not change over time since release and they did not spatially or temporally avoid cats. However, cats were significantly spatially associated with rabbits while quolls were not, suggesting higher predation efficacy in quolls possibly due to their smaller body size enabling them to catch rabbits inside warrens. Despite high dietary overlap and intraguild predation, the quoll’s broad and flexible diet and high predation efficacy appeared to assist in facilitating coexistence and reducing competition in this novel predator interaction. This dietary flexibility may be harnessed to improve conservation outcomes: reducing introduced rabbits in our study area could naturally reduce feral cat populations while having less impact on native quolls.
Landscape dynamic network biomarker analysis reveals the tipping point of transcriptome reprogramming to prevent skin photodamage
Authors: Zhang Chengming , Zhang Hong , Ge Jing , Mi Tingyan , Cui Xiao , Tu Fengjuan , Gu Xuelan , Zeng Tao , Chen Luonan 
Published: September 2021

Abstract
Skin, as the outmost layer of human body, is frequently exposed to environmental stressors including pollutants and ultraviolet (UV), which could lead to skin disorders. Generally, skin response process to ultraviolet B (UVB) irradiation is a nonlinear dynamic process, with unknown underlying molecular mechanism of critical transition. Here, the landscape dynamic network biomarker (l-DNB) analysis of time series transcriptome data on 3D skin model was conducted to reveal the complicated process of skin response to UV irradiation at both molecular and network levels. The advanced l-DNB analysis approach showed that: (i) there was a tipping point before critical transition state during pigmentation process, validated by 3D skin model; (ii) 13 core DNB genes were identified to detect the tipping point as a network biomarker, supported by computational assessment; (iii) core DNB genes such as COL7A1 and CTNNB1 can effectively predict skin lightening, validated by independent human skin data. Overall, this study provides new insights for skin response to repetitive UVB irradiation, including dynamic pathway pattern, biphasic response, and DNBs for skin lightening change, and enables us to further understand the skin resilience process after external stress.
Mitotic inactivation of the cGAS‒MITA/STING pathways
Authors: Zhong Li , Shu Hong-Bing 
Published: September 2021

Abstract
The cyclic guanosine monophosphate‒adenosine monophosphate synthase (cGAS)‒mediator of interferon response factor 3 activation/stimulator of interferon genes (MITA/STING) axis has emerged as a major pathway, which senses microbial or mislocated cellular DNA in the cytosol to trigger innate immune responses. cGAS senses cytosolic DNA without a preference of self- or nonself-DNA. How the cGAS‒MITA/STING axis is inactivated upon nuclear envelope breakdown (NEBD) at mitotic entry in vertebrate cells to avoid self-DNA sensing remains unclear until very recently. In this review, we summarize the recent advances on how cGAS responds to chromosomes upon NEBD and the mechanisms involved in the inactivation of the cGAS‒MITA/STING pathways in mitosis.