bluuebunny/crossref_metadata_2025_split huggingface dataset:
Implementation of Evidence-Based Presurgical Testing Guidelines in Patients Undergoing Ambulatory Surgery for Endometrial Cancer
Authors: Aviki Emeline M. , Gordhandas Sushmita B. , Velzen Jeena , Riley Michael , Manning-Geist Beryl , Rice Jonathan , Weiss Hallie , Abu-Rustum Nadeem R. , Gardner Ginger J. 
Published: July 2021

PURPOSE: The aim of this quality improvement intervention was to evaluate the safety and cost savings of presurgical testing (PST) guidelines for patients undergoing hysterectomy for endometrial pathology in the ambulatory setting. METHODS: Evidence-based presurgical testing (PST) guidelines were developed by a multidisciplinary team. These guidelines were implemented on the gynecologic surgery service of a comprehensive cancer center in January 2016. All patients with a diagnosis of endometrial pathology who underwent ambulatory surgery during the specified time periods were included in this analysis. A pre-post analysis was performed (preperiod, July 2014-December 2015; postperiod, July 2016-December 2017). Rates of completed presurgical tests and perioperative adverse events were compared between time periods. Cost savings related to the reduction in PST were calculated using the direct cost of testing and reported in percentage cost reduction. RESULTS: A total of 749 hysterectomies were completed in the preperiod and 775 in the postperiod. After implementation of PST guidelines, complete blood counts, coagulation testing, comprehensive metabolic panels, chest x-rays, and electrocardiograms were reduced by 13.4%, 78.1%, 36.8%, 39.0%, and 15.5%, respectively (all P < .001). Rates of perioperative cardiopulmonary adverse events (0% v 0%) and hematologic adverse events (3.3% v 2.0%; P = .10) were stable between time periods. There were no deaths within 90 days of surgery. There was a 41.4% reduction in direct costs related to PST in the postperiod. CONCLUSION: The use of evidence-based PST guidelines for patients with endometrial pathology undergoing hysterectomy in the ambulatory setting is safe and cost-effective. A multidisciplinary approach is essential for successful development and implementation.
Clinical Utility of Plasma-Based Comprehensive Molecular Profiling in Advanced Non–Small-Cell Lung Cancer
Authors: Schouten Robert D. , Vessies Daan C. L. , Bosch Linda J. W. , Barlo Nicole P. , van Lindert Anne S. R. , Cillessen Saskia A. G. M. , van den Broek Daan , van den Heuvel Michel M. , Monkhorst Kim 
Published: July 2021

PURPOSE Comprehensive molecular profiling (CMP) plays an essential role in clinical decision making in metastatic non–small-cell lung cancer (mNSCLC). Circulating tumor DNA (ctDNA) analysis provides possibilities for molecular tumor profiling. In this study, we aim to explore the additional value of centralized ctDNA profiling next to current standard-of-care protocolled tissue-based molecular profiling (SoC-TMP) in the primary diagnostic setting of mNSCLC in the Netherlands. METHODS Pretreatment plasma samples from 209 patients with confirmed mNSCLC were analyzed retrospectively using the NGS AVENIO ctDNA Targeted Kit (Roche Diagnostics, Basel, Switzerland) and compared with paired prospective pretreatment tissue-based molecular profiling from patient records. The AVENIO panel is designed to detect single-nucleotide variants, copy-number variations, insertions or deletions, and tyrosine kinase fusion in 17 genes. RESULTS Potentially targetable drivers were detected with SoC-TMP alone in 34.4% of patients. Addition of clonal hematopoiesis of indeterminate potential–corrected, plasma-based CMP increased this to 39.7% ( P < .001). Concordance between SoC-TMP and plasma-CMP was 86.6% for potentially targetable drivers. Clinical sensitivity of plasma-CMP was 75.2% for any oncogenic driver. Specificity and positive predictive value were more than 90% for all oncogenic drivers. CONCLUSION Plasma-CMP is a reliable tool in the primary diagnostic setting, although it cannot fully replace SoC-TMP. Complementary profiling by combined SoC-TMP and plasma-CMP increased the proportion of patients who are eligible for targeted treatment.
Clinical Outcomes of Molecular Tumor Boards: A Systematic Review
Authors: Larson Kara L. , Huang Bin , Weiss Heidi L. , Hull Pam , Westgate Philip M. , Miller Rachel W. , Arnold Susanne M. , Kolesar Jill M. 
Published: July 2021

PURPOSE We conducted this systematic review to evaluate the clinical outcomes associated with molecular tumor board (MTB) review in patients with cancer. METHODS A systematic search of PubMed was performed to identify studies reporting clinical outcomes in patients with cancer who were reviewed by an MTB. To be included, studies had to report clinical outcomes, including clinical benefit, response, progression-free survival, or overall survival. Two reviewers independently selected studies and assessed quality with the Quality Assessment Tool for Before-After (Pre-Post) Studies with No Control Group or the Quality Assessment Tool for Observational Cohort and Cross-Sectional Studies depending on the type of study being reviewed. RESULTS Fourteen studies were included with a total of 3,328 patients with cancer. All studies included patients without standard-of-care treatment options and usually with multiple prior lines of therapy. In studies reporting response rates, patients receiving MTB-recommended therapy had overall response rates ranging from 0% to 67%. In the only trial powered on clinical outcome and including a control group, the group receiving MTB-recommended therapy had significantly improved rate of progression-free survival compared with those receiving conventional therapy. CONCLUSION Although data quality is limited by a lack of prospective randomized controlled trials, MTBs appear to improve clinical outcomes for patients with cancer. Future research should concentrate on prospective trials and standardization of approach and outcomes.
Spatiotemporal control of pathway sensors and cross-pathway feedback regulate a differentiation MAPK pathway in yeast
Authors: Prabhakar Aditi , González Beatriz , Dionne Heather , Basu Sukanya , Cullen Paul J. 
Published: July 2021

ABSTRACT
Mitogen-activated protein kinase (MAPK) pathways control cell differentiation and the response to stress. In Saccharomyces cerevisiae, the MAPK pathway that controls filamentous growth (fMAPK) shares components with the pathway that regulates the response to osmotic stress (HOG). Here, we show that the two pathways exhibit different patterns of activity throughout the cell cycle. The different patterns resulted from different expression profiles of genes encoding mucin sensors that regulate the pathways. Cross-pathway regulation from the fMAPK pathway stimulated the HOG pathway, presumably to modulate fMAPK pathway activity. We also show that the shared tetraspan protein Sho1p, which has a dynamic localization pattern throughout the cell cycle, induced the fMAPK pathway at the mother-bud neck. A Sho1p-interacting protein, Hof1p, which also localizes to the mother-bud neck and regulates cytokinesis, also regulated the fMAPK pathway. Therefore, spatial and temporal regulation of pathway sensors, and cross-pathway regulation, control a MAPK pathway that regulates cell differentiation in yeast.
Riehen, Auf der Bischoffhöhe 13, 1994/12
Authors: Leuzinger Urs 
Published: July 2021

Während der routinemässigen Baustellenüberwachung konnten im Aushub für einen Einfamilienhaus-Neubau zwei Silexartefakte geborgen werden.
Diese beiden Fundstücke stehen im Zusammenhang mit der neolithischen Freilandstation unmittelbar neben der Baustelle.
«Manger & Boire» an der Gerbergasse 81 (1995/23)
Authors: Matt Christoph Ph. 
Published: July 2021

Am kurzen Stichgässlein zwischen der oberen Gerbergasse und dem Barfüsserplatz steht ein altes Haus mit einem auffälligen, weit vorkragenden Viertelwalmdach.
Im Winterhalbjahr 1995/96 konnte die Archäologische Bodenforschung die historische Bausubstanz im Keller an den Mauern und im Boden untersuchen; im Sommer 1996 wurden im Zuge der Umbauten die alten baufälligen Kellerinnenwände und die Kellerdecke entfernt und die nunmehr freigelegten Stellen baubegleitend erforscht.
Zur Parzellenstruktur der Stadt Basel vor 1300
Authors: Matt Christoph Ph. 
Published: July 2021

Die bisher aufgearbeiteten Siedlungsbefunde in der Talstadt und am Rande der Niederterrasse bieten Einblick in Struktur und Gliederung der Stadt. Als Fazit kann festgehalten werden, dass in Basel sowohl Parzellierung wie Hausentwicklung unter anderem stark von den topographischen Voraussetzungen abhängig sind.
Obwohl den Grundstücken in bevorzugter Wohnlage andere Baupläne zugrunde lagen als den nach funktionellen Gesichtspunkten gegliederten Parzellen im Gewerbeviertel, lassen sich gemeinsame Entwicklungstendenzen erkennen. Hier wie dort sind die ältesten Kernbauten hinter älteren Holzhäusern von der Gasse abgesetzt. Etwa ab 1300 werden die gassennahen Bereiche in Stein überbaut. Dieser Ausbau führt zu Parzellenteilungen und teilweise zu einer Umkehr der Nutzung von Hof und Hausteil. Die zuvor nur auf einer Ebene ausgeübten Tätigkeiten - Wohnen im Steinhaus, Werken im Hof, Verkaufen in der Bude an der Strasse - wurden nun in den Vorderhäusern übereinander, auf verschiedenen Stockwerken, ausgeübt. Die Wohngeschosse kamen dabei über die Verkaufsläden und Werkstätten zu liegen.
Lassen sich die jüngeren Bauphasen teilweise heute noch im Stadtbild ablesen , so können die Anfänge nur noch archäologisch festgestellt werden.
Case Report: Systemic lupus erythematous associated with thrombotic thrombocytopenic purpura, a diagnostic challenge
Authors: Suárez-Ale Horacio , Fabian-Aquino Elizabeth , Failoc-Rojas Virgilio E. , Benites-Zapata Vicente A , Ignacio-Cconchoy Felipe 
Published: July 2021

Thrombotic thrombocytopenic purpura (TTP) is an uncommon microangiopathic disease and often occurs as a complication of systemic lupus erythematous (SLE). However, this probable causal relationship has not been completely proven. The diagnostic differentiation of both diseases is difficult in the first instance because they share similar characteristics that may overlap. We present a case of a 32-year-old woman with antecedents of epilepsy since she was 12 years old. The patient was admitted to the emergency room with a clinical picture of headaches, fever, paleness in the skin and mucosa, confused state, paresthesia, and transient spasticity of the extremities. The laboratory results revealed Coombs negative direct autoimmune hemolytic anaemia, severe thrombocytopenia, significant elevation of the enzyme lactate dehydrogenase, and presence of schistocytes ++ in the peripheral film. In addition, positive antinuclear antibodies and positive anti-native DNA in titers of 1/320 and 1/160, respectively, were found. Renal function was conserved. We concluded that it was a case of TTP associated with SLE and indicated treatment with plasmapheresis and methylprednisolone pulses, obtaining a satisfactory response (normalization of biomarker levels, health condition) after the second session of plasmapheresis. Diagnosis of both SLE and TTP is often difficult to achieve; however, adequate correlation of clinical manifestations and laboratory tests, along with the help of partial therapeutic interventions, may lead to good clinical response.
Algorithms for Prediction of Clinical Deterioration on the General Wards: A Scoping Review
Authors: Peelen Roel V , Eddahchouri Yassin , Koeneman Mats , van de Belt Tom H , van Goor Harry , Bredie Sebastian JH 
Published: July 2021

OBJECTIVEThe primary objective of this scoping review was to identify and describe state‐of‐the‐art models that use vital sign monitoring to predict clinical deterioration on the general ward. The secondary objective was to identify facilitators, barriers, and effects of implementing these models.DATA SOURCESPubMed, Embase, and CINAHL databases until November 2020.STUDY SELECTIONWe selected studies that compared vital signs–based automated real‐time predictive algorithms to current track‐and‐trace protocols in regard to the outcome of clinical deterioration in a general ward population.DATA EXTRACTIONStudy characteristics, predictive characteristics and barriers, facilitators, and effects.RESULTSWe identified 1741 publications, 21 of which were included in our review. Two of the these were clinical trials, 2 were prospective observational studies, and the remaining 17 were retrospective studies. All of the studies focused on hospitalized adult patients. The reported area under the receiver operating characteristic curves ranged between 0.65 and 0.95 for the outcome of clinical deterioration. Positive predictive value and sensitivity ranged between 0.223 and 0.773 and from 7.2% to 84.0%, respectively. Input variables differed widely, and predicted endpoints were inconsistently defined. We identified 57 facilitators and 48 barriers to the implementation of these models. We found 68 reported effects, 57 of which were positive.CONCLUSIONPredictive algorithms can detect clinical deterioration on the general ward earlier and more accurately than conventional protocols, which in one recent study led to lower mortality. Consensus is needed on input variables, predictive time horizons, and definitions of endpoints to better facilitate comparative research.
Does Transportation Mean Transplantation? Impact of New Airline Routes on Sharing of Cadaveric Kidneys
Authors: Wang Guihua , Zheng Ronghuo , Dai Tinglong 
Published: July 2021

Every year, nearly 5,000 patients die while waiting for kidney transplants, and yet an estimated 3,500 procured kidneys are discarded. Such a polarized coexistence of dire scarcity and massive wastefulness has been mainly driven by insufficient pooling of cadaveric kidneys across geographic regions. Although numerous policy initiatives are aimed at broadening organ pooling, they rarely account for a key friction—efficient airline transportation, ideally direct flights, is necessary for long-distance sharing, because of the time-sensitive nature of kidney transplantation. Conceivably, transplant centers may be reluctant to accept kidney offers from far-off locations without direct flights. In this paper, we estimate the effect of the introduction of new airline routes on broader kidney sharing. By merging the U.S. airline transportation and kidney transplantation data sets, we create a unique sample tracking (1) the evolution of airline routes connecting all the U.S. airports and (2) kidney transplants between donors and recipients connected by these airports. We estimate the introduction of a new airline route increases the number of shared kidneys by 7.3%. We also find a net increase in the total number of kidney transplants and a decrease in the organ discard rate with the introduction of new routes. Notably, the posttransplant survival rate remains largely unchanged, although average travel distance increases after the introduction of new airline routes. Our results are robust to alternative empirical specifications and have important implications for improving access to the U.S. organ transplantation system. This paper was accepted by Vishal Gaur, operations management.
Ensaio sobre a Economia Fluminense: da crise histórico-estrutural alheada à crise manifesta
Authors: Natal Jorge Luiz Alves , Da Cruz José Luis Vianna 
Published: July 2021

O ensaio examina a denominada crise da economia fluminense, particularmente a da capital – Rio de Janeiro. A tese central defendida é que, em verdade, as crises da economia fluminense são manifestações intermitentes de uma profunda e longeva crise estrutural, e que remontam à passagem do século XIX ao século XX, com alguns interregnos de crescimento e mudanças até a denominada retomada dos anos 1990, e alguns soluços de crescimento na primeira década dos anos 2000 – que os autores chamam de milagrinho, tomando por empréstimo o termo cunhado por Laura Carvalho (2018). Apontando o alheamento em relação à crise estrutural e às abordagens que consideram o território ou região que abrange o interior fluminense, o ensaio enuncia aspectos metodológicos, teóricos e propositivos relativos não só aos diagnósticos como também – e sobretudo – aos encaminhamentos capazes de responder aos desafios colocados para que o estado, em sua inteireza (e integradamente), cresça de maneira sustentada no tempo
Buried 3D spot-size converters for silicon photonics
Authors: Zhang W. , Ebert M. , Reynolds J. D. , Chen B. , Yan X. , Du H. , Banakar M. , Tran D. T. , Littlejohns C. G. , Reed G. T. , Thomson D. J. 
Published: July 2021

In this article, an efficient spot-size converter (SSC) for low-loss optical mode transition between large and small waveguides based upon a buried three-dimensional (3D) taper is demonstrated. The SCC can pave the way for scalable, low-loss coupling between on-chip waveguides of different sizes and with external components such as optical fibers and III-V active components, and it can be a key element in solving the challenges surrounding the economic high volume packaging and assembly of photonic integrated circuits. Through the use of a bespoke fabrication process, continual tapering of the waveguide dimensions both in width and height is achieved, offering minimal perturbance of the optical mode throughout the structure. The SSC exploits the space of the buried oxide (BOX) on a standard silicon-on-insulator wafer, leaving a planar top wafer surface, meaning that, crucially, further processing of the wafer is not inhibited in any way. Fabricated proof-of-concept devices demonstrate coupling between standard single-mode 220 nm thick silicon waveguides and large-core waveguides with dimensions about 3 µm wide and 1.5 µm height with BOX thickness of 2 µm. Coupling losses as low as 0.56 dB are achieved, limited mostly by the material loss of the polysilicon used. Substantial improvements can be yielded by simply changing the infill material and through optimization of the fabrication process and design. The demonstrated SSC approach can further be applied to other photonic platforms such as silicon nitride on insulator and so on.
Reference genes for mesangial cell and podocyte qPCR gene expression studies under high-glucose and renin-angiotensin-system blocker conditions
Authors: Hosni Nicole Dittrich , Anauate Ana Carolina , Boim Mirian Aparecida 
Published: July 2021

Background
Real-time PCR remains currently the gold standard method for gene expression studies. Identification of the best reference gene is a key point in performing high-quality qPCR, providing strong support for results, and performing as a source of bias when inappropriately chosen. Mesangial cells and podocytes, as essential cell lines to study diabetic kidney disease (DKD) physiopathology, demand accurate analysis of the reference genes used thus far to enhance the validity of gene expression studies, especially regarding high glucose (HG) and DKD treatments, with angiotensin II receptor blockers (e.g., losartan) being the most commonly used. This study aimed to evaluate the suitability and define the most stable reference gene for mesangial cell and podocyte studies of an in vitro DKD model of disease and its treatment.

Methods
Five software packages (RefFinder, NormFinder, GeNorm, Bestkeeper, and DataAssist) and the comparative ΔCt method were selected to analyze six different candidate genes: HPRT, ACTB, PGAM-1, GAPDH, PPIA, and B2M. RNA was extracted, and cDNA was synthesized from immortalized mouse mesangial cells and podocytes cultured in 4 groups: control (n = 5; 5 mM glucose), mannitol (n = 5; 30 mM, as osmotic control), HG (n = 5; 30 mM glucose), and HG + losartan (n = 5; 30 mM glucose and 10−4 mM losartan). Real-time PCR was performed according to MIQE guidelines.

Results
We identified that the use of 2 genes was the best combination for qPCR normalization for both mesangial cells and podocytes. For mesangial cells, the combination of HPRT and ACTB presented higher stability values. For podocytes, HPRT and GAPDH showed the best results.

Conclusion
This analysis provides support for the use of HPRT and ACTB as reference genes in mouse mesangial cell studies of gene expression via real-time PCR, while for podocytes, HPRT and GAPDH should be chosen.
Measuring threshold and latency of motion perception on a swinging bed
Authors: Guyon Maxime , Chea Cyrielle , Laroche Davy , Fournel Isabelle , Baudet Audrey , Toupet Michel , Bozorg Grayeli Alexis 
Published: July 2021

IntroductionOur objective was to develop and to evaluate a system to measure latency and threshold of pendular motion perception based on a swinging bed.Materials and methodsThis prospective study included 30 healthy adults (age: 32 ± 12 years). All subjects were tested twice with a 10 min. interval. A second trial was conducted 2 to 15 days after. A rehabilitation swinging bed was connected to an electronic device emitting a beep at the beginning of each oscillation phase with an adjustable time lag. Subjects were blindfolded and auditory cues other than the beep were minimized. The acceleration threshold was measured by letting the bed oscillate freely until a natural break and asking the patient when he did not perceive any motion. The perception latency was determined by asking the patient to indicate whether the beep and the peak of each oscillation were synchronous. The time lag between sound and peak of the head position was swept from -750 to +750 ms by 50 ms increments.ResultsThe mean acceleration threshold was 9.2±4.60 cm/s2. The range width of the synchronous perception interval was estimated as 535±190 ms. The point of subjective synchronicity defined as the center of this interval was -195±106 ms (n = 30). The test-retest evaluation in the same trial showed an acceptable reproducibility for the acceleration threshold and good to excellent for all parameters related to sound-movement latency.ConclusionSwinging bed combined to sound stimulation can provide reproducible information on movement perception in a simple and non-invasive manner with highly reproducible results.