bluuebunny/crossref_metadata_2025_split huggingface dataset:
Failed Eradication Therapy of New-Onset Pseudomonas aeruginosa Infections in Children With Cystic Fibrosis Is Associated With Bacterial Resistance to Neutrophil Functions
Authors: Kwong Kelly , Benedetti Andrea , Yau Yvonne , Waters Valerie , Nguyen Dao 
Published: February 2021

Abstract

Background
Antibiotics, such as inhaled tobramycin, are used to eradicate new-onset Pseudomonas aeruginosa (PA) infections in patients with cystic fibrosis (CF) but frequently fail due to reasons poorly understood. We hypothesized that PA isolates’ resistance to neutrophil antibacterial functions was associated with failed eradication in patients harboring those strains.

Methods
We analyzed all PA isolates from a cohort of 39 CF children with new-onset PA infections undergoing tobramycin eradication therapy, where 30 patients had eradicated and 9 patients had persistent infection. We characterized several bacterial phenotypes and measured the isolates’ susceptibility to neutrophil antibacterial functions using in vitro assays of phagocytosis and intracellular bacterial killing.

Results
PA isolates from persistent infections were more resistant to neutrophil functions, with lower phagocytosis and intracellular bacterial killing compared to those from eradicated infections. In multivariable analyses, in vitro neutrophil responses were positively associated with twitching motility, and negatively with mucoidy. In vitro neutrophil phagocytosis was a predictor of persistent infection following tobramycin even after adjustment for clinical risk factors.

Conclusions
PA isolates from new-onset CF infection show strain-specific susceptibility to neutrophil antibacterial functions, and infection with PA isolates resistant to neutrophil phagocytosis is an independent risk factor for failed tobramycin eradication.
On bivalent semantics and natural deduction for some infectious logics
Authors: Belikov Alex 
Published: February 2021

AbstractIn this work, we propose a variant of so-called informational semantics, a technique elaborated by Voishvillo, for two infectious logics, Deutsch’s ${\mathbf{S}_{\mathbf{fde}}}$ and Szmuc’s $\mathbf{dS}_{\mathbf{fde}}$. We show how the machinery of informational semantics can be effectively used to analyse truth and falsity conditions of disjunction and conjunction. Using this technique, it is possible to claim that disjunction and conjunction can be rightfully regarded as such, a claim which was disputed in the recent literature. Both ${\mathbf{S}_{\mathbf{fde}}}$ and $\mathbf{dS}_{\mathbf{fde}}$ are formalized in terms of natural deduction. This allows us to solve several problems: to develop a natural deduction calculus for ${\mathbf{S}_{\mathbf{fde}}}$ containing the standard form of disjunction elimination (in contrast to the calculus by Petrukhin), to introduce the first natural deduction calculus for $\mathbf{dS}_{\mathbf{fde}}$ and to reflect the fundamental symmetry between ${\mathbf{S}_{\mathbf{fde}}}$ and $\mathbf{dS}_{\mathbf{fde}}$ on proof-theoretical level forming a convenient basis for obtaining their well-known extensions $\mathbf{K}^{\mathbf{w}}_{\mathbf{3}}$ and $\mathbf{PWK}$.
Enzymatic Hydrolysis of Defatted Antheraea pernyi (Lepidoptera: Saturniidae) Pupa Protein by Combined Neutral Protease Yield Peptides With Antioxidant Activity
Authors: Ma Shuhui , Li Xuejun , Sun Yongxin , Mi Rui , Li Yajie , Wen Zhixin , Meng Nan , Yi Li , Du Xingfan , Li Shuying 
Published: February 2021

Abstract
In this study, peptides were prepared from defatted Antheraea pernyi (Lepidoptera: Saturniidae) pupa protein via hydrolysis with combined neutral proteases. Single-factor tests and response surface methodology (RSM) were used to determine the optimal hydrolysis condition suitable for industrial application. Optimal hydrolysis of the defatted pupa protein was found to occur at an enzyme concentration of 4.85 g/liter, a substrate concentration of 41 g/liter, a hydrolysis temperature of 55°C, and a hydrolysis time of 10 h and 40 min. Under these conditions, the predicted and actual rates of hydrolysis were 45.82% and 45.75%, respectively. Peptides with a molecular weight of less than 2,000 Da accounted for 90.5% of the total peptides generated. Some of the peptides were antioxidant peptides as revealed by sequencing and functional analysis. The antioxidant activity of the mixed peptides was subsequently confirmed by an antioxidant activity assay. The results showed that peptides with high antioxidant activity could be obtained from the hydrolysis of A. pernyi pupa protein.
Enhancement of Temephos and Deltamethrin Toxicity by Petroselinum crispum Oil and its Main Constituents Against Aedes aegypti (Diptera: Culicidae)
Authors: Junkum Anuluck , Intirach Jitrawadee , Chansang Arpaporn , Champakaew Danita , Chaithong Udom , Jitpakdi Atchariya , Riyong Doungrat , Somboon Pradya , Pitasawat Benjawan 
Published: February 2021

Abstract
Previous work presented the profound antimosquito potential of Petroselinum crispum essential oil (PEO) against either the pyrethroid-susceptible or resistant strains of Aedes aegypti. This plant oil also inhibited the activity of acetylcholinesterase and mixed-function oxidases significantly, thus suggesting its potential as a synergist for improving mosquitocidal efficacy of insecticidal formulations. This study investigated the chemical composition, larvicidal activity, and potential synergism with synthetic insecticides of PEO and its main compounds for the purpose of interacting with insecticide resistance in mosquito vectors. The chemical profile of PEO, obtained by GC-MS analysis, showed a total of 17 bioactive compounds, accounting for 99.09% of the whole oil, with the most dominant constituents being thymol (74.57%), p-cymene (10.73%), and γ-terpinene (8.34%). All PEO constituents exhibited promising larvicidal effects, with LC50 values ranging from 19.47 to 59.75 ppm against Ae. aegypti, in both the pyrethroid-susceptible and resistant strains. Furthermore, combination-based bioassays revealed that PEO, thymol, p-cymene, and γ-terpinene enhanced the efficacy of temephos and deltamethrin significantly. The most effective synergist with temephos was PEO, which reduced LC50 values to 2.73, 4.94, and 3.28 ppb against MCM-S, PMD-R, and UPK-R, respectively, with synergism ratio (SR) values of 1.33, 1.38, and 2.12, respectively. The best synergist with deltamethrin also was PEO, which reduced LC50 values against MCM-S, PMD-R, and UPK-R to 0.008, 0.18, and 2.49 ppb, respectively, with SR values of 21.25, 9.00, and 4.06, respectively. This research promoted the potential for using essential oil and its principal constituents as not only alternative larvicides, but also attractive synergists for enhancing efficacy of existing conventional insecticides.
Differences between species in seed bank and vegetation helps to hold functional diversity in a floodable Neotropical savanna
Authors: de Souza Evaldo B , Bao Francielli , Damasceno Junior Geraldo A , Pott Arnildo 
Published: February 2021

AbstractAimsOur objective was to quantify the contributions of the seed bank and the established vegetation to the species composition, functional composition and diversity, and discuss the implications of these differences in regeneration and persistence of floodplain plant communities.MethodsWe sampled all ground cover vegetation up to 1.5 m height and seed bank in 25 plots (10 m × 1 m) distributed across five sites in dry and rainy seasons in a periodically flooded savanna in the Pantanal wetland, Brazil. We evaluated the soil seed bank by seedling emergence method.Important FindingsThe seed bank species had traits that conferred regeneration to the communities, while persistence traits characterized the vegetation. The seed bank had higher functional richness and lower functional evenness than the vegetation. The existence of different plant traits between seed bank and vegetation allowed the coexistence of species with functionally contrasting persistence and regeneration traits, which may help maintain functional diversity. It may allow the community to be more resilient when dealing with different environmental filters such as drought, fire and flood.
Factor Structure of Medical Autonomy Scales in Young People with Spina Bifida
Authors: Smith Zoe R , Holmbeck Grayson N 
Published: February 2021

Abstract

Objective
The primary goal of this study was to examine the factor structure of a spina bifida (SB) medical responsibilities measure and a medical regimen skills scale across time in families of youth with SB.

Method
One-hundred and forty youth with SB and their parents were assessed in both childhood/adolescence and adolescence/young adulthood. The Sharing of SB Medical Responsibilities Scale (SOSBMR) includes 34 items for which participants indicate who is responsible for each task. The SB Independence Survey (SBIS) is composed of 50 SB-specific medical skills items in yes-no format. Confirmatory factor analyses (CFA) were conducted to examine the factor structure of the SOSBMR and SBIS in childhood and adolescence (ages 8–15) and in adolescence/young adulthood (AYA; ages 16–25).

Results
One- and seven-factor CFAs were compared for both measures. For the SBIS, both mother- and father-report were used in childhood; self-report was employed for AYA. For the SOSBMR, only self-report was used for both age groups. Across each rater and time point, the seven-factor models of the SBIS and SOSBMR had adequate to excellent fit and reliability, indicating the ability to use each subscale. In addition, each of the corresponding subscales on the SOSBMR and SBIS were associated with each other across raters and time, showing good concurrent and predictive validity.

Conclusions
From childhood to young adulthood, the subscales of the SOSBMR can be used to examine responsibility across multiple medical tasks and the SBIS can be used to assess medical regimen skills and mastery in young people with SB.
A rare case of post-traumatic high-flow priapism requiring endovascular salvage with bilateral superselective microcoil embolization
Authors: Williams Aman B , Lax Lauren G 
Published: February 2021

Abstract
Post-traumatic high-flow priapism is a rare occurrence, with potentially debilitating long-term erectile dysfunction if left unaddressed. Even rarer, however, is for the priapism symptoms to be caused by a single cavernosal arterial pseudoaneurysm, with feeding vessels from the distal branched vessels of ‘both’ the left and right internal pudendal arteries. To the best of our knowledge, we present the first documented case of endovascular salvage utilizing superselective microcoil embolization in the treatment of high-flow priapism caused by a singular pseudoaneurysm with bilateral inflow. Timing of symptoms, interpretation of imaging, multidisciplinary discussions, procedural risk, arterial anatomy and choice of embolic agent were all careful considerations in this case. Following embolization, this young gentleman ultimately had a successful angiographic result, normalization of his cavernosal artery peak systolic velocity on ultrasound and a full return to normal erectile function by 6 months.
Predicting Nonresponse in Future Waves of a Probability-Based Mixed-Mode Panel with Machine Learning
Authors: Kern Christoph , Weiß Bernd , Kolb Jan-Philipp 
Published: February 2021

Abstract
Nonresponse in panel studies can lead to a substantial loss in data quality owing to its potential to introduce bias and distort survey estimates. Recent work investigates the usage of machine learning to predict nonresponse in advance, such that predicted nonresponse propensities can be used to inform the data collection process. However, predicting nonresponse in panel studies requires accounting for the longitudinal data structure in terms of model building, tuning, and evaluation. This study proposes a longitudinal framework for predicting nonresponse with machine learning and multiple panel waves and illustrates its application. With respect to model building, this approach utilizes information from multiple waves by introducing features that aggregate previous (non)response patterns. Concerning model tuning and evaluation, temporal crossvalidation is employed by iterating through pairs of panel waves such that the training and test sets move in time. Implementing this approach with data from a German probability-based mixed-mode panel shows that aggregating information over multiple panel waves can be used to build prediction models with competitive and robust performance over all test waves.
When junk DNA turns functional: transposon-derived non-coding RNAs in plants
Authors: Ariel Federico D , Manavella Pablo A 
Published: February 2021

AbstractTransposable elements (TEs) are major contributors to genome complexity in eukaryotes. TE mobilization may cause genome instability, although it can also drive genome diversity throughout evolution. TE transposition may influence the transcriptional activity of neighboring genes by modulating the epigenomic profile of the region or by altering the relative position of regulatory elements. Notably, TEs have emerged in the last few years as an important source of functional long and small non-coding RNAs. A plethora of small RNAs derived from TEs have been linked to the trans regulation of gene activity at the transcriptional and post-transcriptional levels. Furthermore, TE-derived long non-coding RNAs have been shown to modulate gene expression by interacting with protein partners, sequestering active small RNAs, and forming duplexes with DNA or other RNA molecules. In this review, we summarize our current knowledge of the functional and mechanistic paradigms of TE-derived long and small non-coding RNAs and discuss their role in plant development and evolution.
Clade III cytokinin response factors share common roles in response to oxidative stress responses linked to cytokinin synthesis
Authors: Hughes Ariel M , Hallmark H Tucker , Plačková Lenka , Novák Ondrej , Rashotte Aaron M 
Published: February 2021

Abstract
Cytokinin response factors (CRFs) are transcription factors that are involved in cytokinin (CK) response, as well as being linked to abiotic stress tolerance. In particular, oxidative stress responses are activated by Clade III CRF members, such as AtCRF6. Here we explored the relationships between Clade III CRFs and oxidative stress. Transcriptomic responses to oxidative stress were determined in two Clade III transcription factors, Arabidopsis AtCRF5 and tomato SlCRF5. AtCRF5 was required for regulated expression of &gt;240 genes that are involved in oxidative stress response. Similarly, SlCRF5 was involved in the regulated expression of nearly 420 oxidative stress response genes. Similarities in gene regulation by these Clade III members in response to oxidative stress were observed between Arabidopsis and tomato, as indicated by Gene Ontology term enrichment. CK levels were also changed in response to oxidative stress in both species. These changes were regulated by Clade III CRFs. Taken together, these findings suggest that Clade III CRFs play a role in oxidative stress response as well as having roles in CK signaling.
Is Serum-Ascites Vitamin D Gradient a Valid Marker for Diagnosing Spontaneous Bacterial Peritonitis in Patients with Cirrhotic Ascites?
Authors: Abdel Hafez Hanan , Madani Hanan , Abdel Alem Shereen , Farrag Ahmed , Fathy Wael , Abdo Mahmoud 
Published: February 2021

Abstract

Objective
Spontaneous bacterial peritonitis (SBP) is considered the paradigmatic model of infection in patients with liver cirrhosis. Therefore, there is a need for an accurate and rapid method for SBP diagnosis. The aim of this study was to evaluate the validity of serum-ascites 25-hydroxyvitamin D (25-OH vitamin D) gradient (SADG) as a marker for diagnosing SBP in patients with cirrhotic ascites.

Methods
We conducted a cross-sectional analytic study of 88 patients with portal hypertensive ascites resulting from liver cirrhosis of any etiology. The demographic, clinical, and laboratory characteristics of the patients were recorded. The level of 25-OH vitamin D in serum and ascitic fluid was measured using high-performance liquid chromatography autoanalyzer. The SADG was calculated with the formula: 25-OH vitamin D in serum − 25-OH vitamin D in ascites.

Results
Vitamin D deficiency was detected in 89.8% of the studied patients. The SADG values ranged between 0 and 69.2 ng/mL, with a median value of 5.58 ng/mL. It was significantly lower in patients with SBP than in those without SBP (P = .004). The area under the curve for SADG in exclusion of SBP was 0.67 at a cutoff value of ≥5.57 ng/mL.

Conclusion
We found that SADG may be a valid marker of SBP in patients with cirrhotic ascites.
RINGO3 polarimetry of very young ZTF supernovae
Authors: Maund J R , Yang Y , Steele I A , Baade D , Jermak H , Schulze S , Bruch R , Gal-Yam A , Höflich P A , Ofek E , Wang X , Amenouche M , Dekany R , Masci F J , Riddle R , Soumagnac M T 
Published: February 2021

ABSTRACT
The early phases of the observed evolution of the supernovae (SNe) are expected to be dominated by the shock breakout and ‘flash’ ionization of the surrounding circumstellar medium. This material arises from the last stages of the evolution of the progenitor, such that photometry and spectroscopy of SNe at early times can place vital constraints on the latest and fastest evolutionary phases leading up to stellar death. These signatures are erased by the expansion of the ejecta within ∼5 d after explosion. Here we present the earliest constraints, to date, on the polarization of 10 transients discovered by the Zwicky Transient Facility (ZTF), between 2018 June and 2019 August. Rapid polarimetric follow-up was conducted using the Liverpool Telescope RINGO3 instrument, including three SNe observed within &lt;1 d of detection by the ZTF. The limits on the polarization within the first 5 d of explosion, for all SN types, is generally $\lt 2{ per\ cent}$, implying early asymmetries are limited to axial ratios &gt;0.65 (assuming an oblate spheroidal configuration). We also present polarimetric observations of the Type I Superluminous SN 2018bsz and Type II SN 2018hna, observed around and after maximum light.
Physical and kinematic conditions of the local merging galaxy NGC 1487
Authors: Buzzo M L , Ziegler B , Amram P , Verdugo M , Barbosa C E , Ciocan B , Papaderos P , Torres-Flores S , Mendes de Oliveira C 
Published: February 2021

ABSTRACT
We present optical VLT/MUSE integral field spectroscopy data of the merging galaxy NGC 1487. We use fitting techniques to study the ionized gas emission of this merger and its main morphological and kinematical properties. We measured flat and sometimes inverted oxygen abundance gradients in the subsystems composing NGC 1487, explained by metal mixing processes common in merging galaxies. We also measured widespread star-forming bursts, indicating that photoionization by stars is the primary ionization source of the galaxy. The kinematic map revealed a rotating pattern in the gas in the northern tail of the system, suggesting that the galaxy may be in the process of rebuilding a disc. The gas located in the central region has larger velocity dispersion (σ ≈ 50 km s−1) than the remaining regions, indicating kinematic heating, possibly owing to the ongoing interaction. Similar trends were, however, not observed in the stellar velocity dispersion map, indicating that the galaxy has not yet achieved equilibrium, and the nebular and stellar components are still kinematically decoupled. Based on all our measurements and findings, and specially on the mass estimates, metallicity gradients, and velocity fields of the system, we propose that NGC 1487 is the result of an ongoing merger event involving smallish dwarf galaxies within a group, in a pre-merger phase, resulting in a relic with mass and physical parameters similar to a dwarf galaxy. Thus, we may be witnessing the formation of a dwarf galaxy by merging of smaller clumps at z = 0.
Successive common envelope events from multiple planets
Authors: Chamandy Luke , Blackman Eric G , Nordhaus Jason , Wilson Emily 
Published: February 2021

ABSTRACT
Many stars harbour multiplanet systems. As these stars expand late in their evolutions, the innermost planet may be engulfed, leading to a common envelope (CE) event. Even if this is insufficient to eject the envelope, it may expand the star further, causing additional CE events, with the last one unbinding what remains of the envelope. This multiplanet CE scenario may have broad implications for stellar and planetary evolution across a range of systems. We develop a simplified version and show that it may be able to explain the recently observed planet WD 1856 b.
Dietary Diversification and Specialization in Neotropical Bats Facilitated by Early Molecular Evolution
Authors: Potter Joshua H T , Davies Kalina T J , Yohe Laurel R , Sanchez Miluska K R , Rengifo Edgardo M , Struebig Monika , Warren Kim , Tsagkogeorga Georgia , Lim Burton K , dos Reis Mario , Dávalos Liliana M , Rossiter Stephen J 
Published: February 2021

Abstract
Dietary adaptation is a major feature of phenotypic and ecological diversification, yet the genetic basis of dietary shifts is poorly understood. Among mammals, Neotropical leaf-nosed bats (family Phyllostomidae) show unmatched diversity in diet; from a putative insectivorous ancestor, phyllostomids have radiated to specialize on diverse food sources including blood, nectar, and fruit. To assess whether dietary diversification in this group was accompanied by molecular adaptations for changing metabolic demands, we sequenced 89 transcriptomes across 58 species and combined these with published data to compare ∼13,000 protein coding genes across 66 species. We tested for positive selection on focal lineages, including those inferred to have undergone dietary shifts. Unexpectedly, we found a broad signature of positive selection in the ancestral phyllostomid branch, spanning genes implicated in the metabolism of all major macronutrients, yet few positively selected genes at the inferred switch to plantivory. Branches corresponding to blood- and nectar-based diets showed selection in loci underpinning nitrogenous waste excretion and glycolysis, respectively. Intriguingly, patterns of selection in metabolism genes were mirrored by those in loci implicated in craniofacial remodeling, a trait previously linked to phyllostomid dietary specialization. Finally, we show that the null model of the widely-used branch-site test is likely to be misspecified, with the implication that the test is too conservative and probably under-reports true cases of positive selection. Our findings point to a complex picture of adaptive radiation, in which the evolution of new dietary specializations has been facilitated by early adaptations combined with the generation of new genetic variation.