bluuebunny/crossref_metadata_2025_split huggingface dataset:
VGLL4 Promotes EV71 Virus Proliferation and Accelerates the Apoptosis of Infected RD Cells
Authors: Wang Shengyu , Sun Xin , Qiao Junhua , He Cancan , Tian Langfei , Chen Yaping 
Published: March 2021

Abstract
Enterovirus 71 (EV71) is one of the major pathogens causing hand, foot and mouth disease (HFMD) which affects public health increasingly. Apoptosis plays an important role in EV71 infection, but the molecular mechanism involved in EV71 induced apoptosis is not completely clear. VGLL4 is a multifunctional protein in host cells, which has been studied in tumor and cell apoptosis, but has not been reported in pathogen. In this study, the mammalian eukaryotic expression plasmid of VGLL4 fused with HA tag (HA-VGLL4) and the model of overexpression VGLL4 RD cells were successfully constructed. The effect of VGLL4 on the proliferation of EV71 was detected by western-blot assay, fluorescence quantitative PCR and cytotoxicity assay (CCK assay), and the mechanism of its effect on the proliferation of the virus was researched. The experimental results showed that VGLL4 may promote the replication of EV71 by promoting the apoptosis of infected cells. VGLL4 can be an important target for prevention and treatment of EV71 infection.
Gradient iterative algorithm for rational models based on Gram-Schmidt orthogonalization method
Authors: Pu Yan , Chen Jing , Yang Yongqing , Zhu Quanmin 
Published: March 2021

Abstract
An improved gradient iterative algorithm, termed as Gram-Schmidt orthogonalization based gradient iterative algorithm, is proposed for rational models in this paper. The algorithm can obtain the optimal parameter estimates in one iteration for the reason that the information vectors obtained by using the Gram-Schmidt orthogonalization method are independent of each other. Compared to the least squares algorithm and the traditional gradient iterative algorithm, the proposed algorithm does not require the matrix inversion and eigenvalue calculation, thus it can be applied to nonlinear systems with complex structures or large-scale systems. Since the information vector of the rational models contains the latest output that is correlated with the noise, a biased compensation Gram-Schmidt orthogonalization based gradient iterative algorithm is introduced, by which the unbiased parameter estimates can be obtained. Two simulated examples are applied to demonstrate the efficiency of the proposed algorithm.
Type-2 Fuzzy Modeling and Control of Nonlinear Steer-by-Wire Systems With Actuator Fault and Event-Triggered Communication
Authors: Ma Bingxin , Wang Yongfu 
Published: March 2021

Abstract
This paper proposes the adaptive fuzzy modeling and control method for steer-by-wire (SbW) systems with uncertain nonlinearity, time-varying disturbance, actuator fault, and event-triggered communication. First, the adaptive interval type-2 fuzzy logic system (IT2 FLS) is developed to modeling the uncertain nonlinearity of SbW systems. The Lyapunov-based adaptive law can guarantee the modeling performance of IT2 FLS. Then, considering the limited communication channel bandwidth of the controller-area-network (CAN), the time-varying disturbance, and actuator fault of SbW systems, an event-triggered sliding mode control is proposed for SbW systems. The chattering phenomenon of the sliding mode control system can be eliminated by using the nested adaptive technology. Theoretical analysis shows that the practical finite-time stability of the closed-loop system can be achieved while avoiding the Zeno-behavior. Finally, numerical simulations and vehicle experiments are given to evaluate the effectiveness of the proposed methods.
Surveillance of Borrelia Miyamotoi-Carrying Ticks&nbsp;and Genomic Analysis of Isolates in Inner Mongolia, China
Authors: Gaowa H , Wulantuya W , Sato Kozue , Liu Dan , Cui Yunhong , Yin Xuhong , Zhang Lihua , Li Hong , Wang Tingfu , Liu Rongxin , Wu Lijing , Lu Saixia , Gao Ting , Zhang Zitong , Cao Minzhi , Wang Guodong , Li Chunpu , Yan Dacheng , Ohashi Norio , Ando Shuji , Kawabata Hiroki 
Published: March 2021

Abstract
Background: Borrelia miyamotoi is a newly described relapsing fever spirochete transmitted by Ixodid tick species. Little is known about the prevalence of B. miyamotoi infections in humans and ticks in Inner Mongolia, China. Therefore, we investigated the prevalence of B. miyamotoi in Ixodes persulcatus, and we aimed to isolate B. miyamotoi from I. persulcatus ticks from four regions of Greater Hinggan, Inner Mongolia, China.Methods: During May to June of 2016-2019, host-seeking adult ticks of I. persulcatus were collected from vegetation. Genomic DNA was prepared from half of each tick body for PCR template, and the remaining half was used to cultivate B. miyamotoi in BSK-M medium. We employed quantitative real-time PCR (qPCR) to detect Borrelia DNA in the ticks and to calculate the prevalence of B. miyamotoi and other borreliae infections. For the characterization of isolated B. miyamotoi, we performed draft genome sequencing and multi-loci sequencing analysis (MLSA). Results: A total of 2,656 I. persulcatus adult ticks were collected. The over-all prevalence of relapsing fever (RF) borreliae in ticks was 5.0% (134/2,656) and that of Lyme disease (LD) borreliae was 43.8% (1,164/2,656). Co-infection by RF and LD borreliae was observed in 63 ticks (2.4%). Ticks that were positive for RF borreliae on qPCR were subjected to glycerophosphodiester diester phosphodiesterase gene (glpQ) PCR amplification and sequencing, through which we identified the RF borreliae specimens as B. miyamotoi. In this study, we successfully isolated the B. miyamotoi strain Hetao-1 from I. persulcatus, and a draft genome sequence was obtained for the isolate. Genomic sequencing revealed the strain Hetao-1 genome to be approximately 906.1 kbp in length (28.9% average GC content), and MLSA analysis identified the strain as ST633, which was previously reported in Japan and Mongolia.Conclusion: We detected B. miyamotoi from I. persulcatus ticks collected in Inner Mongolia, and successfully isolated a B. miyamotoi strain. To our knowledge, this is the first study to culture a B. miyamotoi isolate in China. The data on the prevalence of B. miyamotoi and other borreliae in I. persulcatus ticks will be fundamental for future epidemiological studies of B. miyamotoi disease in Inner Mongolia.
Association of TB treatment with pregnancy complications: A Systematic Review and Meta-analysis Protocol
Authors: Kruger Stefanie Johanna Maria , PHOSWA WENDY 
Published: March 2021

Abstract
Introduction: Tuberculosis is a worldwide health risk factor, especially among immunocompromised groups such as in pregnant women. Diagnosis for TB is complex and appropriate initiation of treatment must be timely and cannot be postponed. This systematic review aims to assess the impact of TB drug exposure linked with pregnancy complications.Methods: Electronic databases (PubMed, Google Scholar, Elsevier and the Cochrane Library) will be screened that covers original articles published from 2010 to 2020, using medical subject headings (MeSH) and free text searches. Population study of TB-infected pregnant women with control being non-infected pregnant women, defined by maternal age between 15 ≤ 44 years, which reported pregnancy outcomes after exposure to TB treatment during pregnancy will be included. PICOS for research question eligibility, PRISMA-P guidelines and flow diagram will be adhered to and assessed by two independent reviewers. Software manager Zotero v5.0.81 will be used to eliminate duplicates and assess eligibility criteria.Ethics and dissemination: We anticipate finding a large number of studies reporting on the impact of TB drugs on the incidence of pregnancy complications which, once summarised, will be useful to establishing the link between TB drugs and pregnancy complications induced by these drugs. The protocol for the systematic review will be registered in PROSPERO. The study will be disseminated electronically and in print. It will also be presented to conferences related to TB and pregnancy.Trial registration: PROSPERO CRD42021226233; Registered on 14 January 2021
The 3D Instance Segmentation Network for Synapse Reconstruction from Serial Electron Microscopy Images
Authors: Liu Jing , Hong Bei , Xie Qiwei , Liu Chao , Han Hua 
Published: March 2021

Abstract
Background The synapse is the key part where neurons communicate with each other. Synaptic plasticity plays a vital role in study and memory. Due to the rapid development of electron microscopy (EM) technology, imaging synapses at nanometer scale possible become possible. However, the automation and effectiveness of the synapse detection algorithm have not yet been satisfactory. The most commonly used method is a two-step solution, where first binary segmentation masks are obtained and then reconstruction results are generated by finding connected components. Results In this paper, a novel 3D instance segmentation network which can predict the synapses end to end was proposed. Then, it was also proved that the network can exploit features consistent with the biological structures of synapses by visualizing the network layer. Furthermore, our method was evaluated on two public datasets, and experimental results demonstrated the effectiveness of our proposed method. Conclusion The proposed method provided a fast and accurate solution to detecting synapses from serial section EM images. Besides, a block-wise inference strategy which adapts well to large scale EM images was introduced, and it can also help neuroscientists achieve labor-free analysis and quantification of synapses.
The Decline of Early Marriage and Motherhood in Latin America: Decomposing Second Demographic Transition Characteristics
Authors: Butler Zackery 
Published: March 2021

Abstract
BackgroundUntil recently, marriage was nearly universal, and occurred early in life for women in Latin America. A significant proportion of women were both married and mothers before reaching adulthood. Girls married under the age of 18 are victims of Child Early and Forced Marriage, a violation of their human rights. The changes predicted by Second Demographic Transition theory may have reduced the prevalence of early marriage and motherhood as well as decoupled the institution of marriage from motherhood. This article contributes to the understanding of early marriage and motherhood in Latin America by describing the relationship between these characteristics and the emergence the Second Demographic Transition among young women from 1980-2010. MethodsI use CEPALSTAT data on marriage and motherhood in Latin America to calculate statistics on marriage and motherhood for young women in the three youngest age groups, those ages 10–14, 15–19, and 20–24. Using these statistics, I assess changes in three classic SDT dimensions (Postponement of Marriage, Postponement of Motherhood, and increased Unmarried Motherhood). I then decompose the Overall Motherhood Prevalence Rate to determine which contributed more to the changes observed, the postponement of marriage, or the postponement of motherhood. ResultsSustained decreases in the prevalence of marriage and motherhood were observed for all groups, but also an increase in unmarried mothers. Declines in marriage were largely responsible for declines in motherhood rates for all age groups in all decades. The changes observed in the data correspond to the SDT predictions and indicate that postponement of marriage is driving the postponement of motherhood for young women in Latin America. Reducing the prevalence of CEFM has played a major role in reducing early motherhood during this transition.ConclusionThis article provides a novel approach to evaluating the impact of the SDT on early marriage and motherhood. It shows that the prevalence of CEFM declined dramatically during this period, although the absolute numbers of child brides increased. The results provide evidence of postponement of marriage and motherhood, the decoupling of marriage and motherhood, and the potential for sustained fertility decline in Latin America.
Estimation of arm kinematics in stroke survivors using wearable sensors
Authors: Nie Jeffrey Z. , Nie James W. , Hung Na-Teng , Cotton R. James , Slutzky Marc W. 
Published: March 2021

Abstract
BackgroundStroke is the leading cause of long-term disability in the United States, often resulting in upper extremity (UE) motor impairment. Most existing outcome metrics of UE function in rehabilitation are insensitive to change or subject to observer bias. There is growing interest in using movement kinematics to measure UE motor function, since they can provide high-resolution, quantitative measurements. However, measuring arm kinematics in stroke survivors, particularly in the hospital or clinic, can be challenging for traditional optical tracking systems due to non-ideal environments, expense, and a limited ability to perform required calibration poses. The aim of this study was to develop a general framework for accurate measurements of wrist position during reaching movements in people with stroke using relatively inexpensive wearable sensors.MethodsWe developed and presented two methods, one using inertial measurement units (IMUs) and using virtual reality (Vive) sensors, that practically estimate wrist position with respect to the shoulder. We then assessed the estimation accuracies of each method during a 3D reaching task by using a Vicon motion capture system. We also demonstrated each methods ability to track two kinematic metrics, sweep area and smoothness, in chronic stroke survivors. We computed Pearson correlation coefficients when appropriate.ResultsCompared to a traditional optical system, both systems tracked with high accuracy during 3D reaching, with mean absolute errors of 1.00 ± 0.80 cm and 1.09 ± 0.51 cm for the IMU and Vive, respectively. Furthermore, both methods’ estimated kinematics highly agreed with each other (p < 0.01).ConclusionsThese methods may be useful for developing kinematic metrics to evaluate stroke rehabilitation outcomes in both laboratory and clinical environments.Trial RegistrationThe clinical trial (ClinicalTrial.gov ID: NCT03401762) was registered on January 17, 2018 (https://clinicaltrials.gov/ct2/show/NCT03401762) and posted on January 1, 2018. The trial is scheduled to be completed by August 2023. The trial was updated on December 2, 2020 and is currently recruiting.
Indications and Outcomes of Emergency Obstetric Hysterectomy; a 5-Year Review at the Bafoussam Regional Hospital, Cameroon
Authors: Mbakwa Mbakwa Rickeins , Tendongfor Nicholas , Ngunyi Yannick Lechedem , Ngek Ekongefeyin Sintieh Nchinda , Alemkia Frank , Egbe Thomas Obinchemti 
Published: March 2021

Abstract
Background: Emergency Obstetric Hysterectomy (EOH) is removal of the uterus due to life threatening conditions within the puerperium. This life saving intervention is associated with life threatening complications. In our setting, little is known on EOH. Objectives: To determine the prevalence, indications and outcomes of emergency obstetric hysterectomy while comparing both postpartum hysterectomy and caesarean hysterectomy. Methods: A 5-year hospital-based retrospective cohort study of medical records of patients who underwent emergency obstetric hysterectomies was carried out at the Bafoussam Regional Hospital (BRH) from 1 st January 2015 to 31 st December 2019. Cases were classified as caesarean hysterectomy (CH) or postpartum hysterectomy (PH). Epidemiological data, indications, and complications of EOH were collected and analyzed in EPI-INFO 7.2.2.1. The chi-squared test was used to compare the two groups, and bivariate analysis was used to identify indicators of adverse outcomes of EOH. Statistical significance was set at p < 0.05 Results: There were 30 cases of emergency obstetric hysterectomy (24 caesarean hysterectomies and 6 postpartum hysterectomies), giving a prevalence rate of 3.75 per 1000 deliveries. The most common indication for CH, was intractable postpartum haemorrhage and uterine rupture (33.33% each), while abnormal placentation (50%) was commonly indicated for PH. Anaemia (both groups) (p=0.013) and sepsis (PH group only, 33.33%) (p=0.03) were the most statistically significant complications of EOH respectively. Absence of blood transfusion prior to surgery (p=0.013) and prolonged surgery lasting 2 or more hours (p=0.04), were significantly associated with a negative clinical outcome. Conclusion: The prevalence of EOH is high. There were no differences in the sociodemographic profile, risk factors and indications of both groups. PH group was more likely to develop sepsis as complication. Lack of blood transfusion prior to surgery and prolonged surgeries were significantly associated to complication. Meticulous care and timely recognition of negative prognostic factors of delivery as well as those of EOH will help improve maternal outcomes of pregnancy.
An integrated software architecture for the pollution-based real-time control of urban drainage systems
Authors: Romero Luis , Joseph-Duran Bernat , Sun Congcong , Meseguer Jordi , Cembrano Gabriela , Guasch Ramón , Martínez Montse , Muñoz Eduard , Puig Vicenç 
Published: March 2021

Abstract
This paper presents a complete methodology for the development of an integrated software architecture, which can achieve a closed-loop application between the integrated real-time control (RTC) and a virtual reality simulation for the urban drainage system (UDS). Quality measurements are considered during the simulation and optimization process. Model predictive control (MPC) and rule-based control (RBC) are the two main RTC methods embedded in this architecture. The proposed integration environment allows the different software components to efficiently and effectively communicate and work in a system-wide way, as well as to execute all the necessary steps regarding input parameter management, scenario configuration and results extraction. The proposed approaches are implemented into a pilot based on the Badalona UDS (Spain). Results from different scenarios with individual control approaches and rain episodes are evaluated and discussed.
Structural and Dynamic Analysis of the Human Capital Health Component of the Socio-Economic System
Authors: Ketova K. , Vavilova D. D. 
Published: March 2021

Purpose of the research.Human capital in the modern world is the primary factor in ensuring the progressive development of society. The most important component of human capital is the component of health, which affects the quality of labor resources and labor productivity in the region. The purpose of this research is to study the development trends of the health component of human capital, using the example of the socio-economic system of one of the regions of the Russian Federation – the Udmurt Republic, to study the structure and dynamics of the specified object of research.Materials and methods.Statistical methods and techniques for studying the development of regional socio-economic processes as an information-supported element in making managerial decisions in strategic planning were used instrumental in this research. The information resource is based on official statistical data of institutions and departments of the Russian Federation and the data from periodicals, as well as using expert assessments in their information and analytical materials. The research base is the socio-economic processes of the last two decades in the Udmurt Republic. A numerical analysis of the structure and dynamics of the state of health of the population is carried out on the example of the Udmurt Republic, using primary modern data reflected in the system of state statistical accounting for the period 2000-2018.Results.Calculations have shown that the quality of the health component of human capital in the Udmurt Republic has been declining in recent decades. The share of healthy people in the region in the working age group of the population 15-72 years old decreased from 59.8% in 2000 to 42.1% in 2018. At the same time, the proportion of people with chronic diseases increased: 33.2% in 2000 and 48.5% in 2018. In general, the share of people with disabilities in the total population of the age group 15-72 years old increased from 7.0% to 9.4%. The general level of health of the population is declining at an average annual rate of 0.4 percentage points. This situation is due to both the aging factor of the population and the general trend of deteriorating health of the population in middle ages.Conclusion.The revealed and analyzed trends in the work of the structure and dynamics of the human capital health component of the population in socio-economic system indicate a decrease in the rate of positive influence of human capital on economic dynamics and the labor market. The analysis showed the emergence of the need to create additional conditions to reduce the level of general morbidity and disability. The results obtained indicate the need to increase the volume of funding for the health care system in order to expand the scale of involvement of the population in a healthy lifestyle, develop a preventive health care system, improve the availability and quality of medical care.
Ovarian Hyperresponse Following the Sole Administration of GnRH
Agonist
Authors: Chen Cheng , Geng Lan , Hou Zhenhui , Liu Dan , Meng Fanhua , Ma Wenmin , Xia Xi 
Published: March 2021

Background :
The gonadotrophin-releasing hormone agonist (GnRHa) has gained widespread popularity of achieving pituitary suppression before ovarian stimulation with exogenous gonadotropins in assisted reproductive technology protocols. However, a very small part of patients may develop ovarian hyperresponse after the sole administration of GnRHa without gonadotropins.

Case report:
A 32-year-old female diagnosed with polycystic ovary syndrome presented for her second IVF cycle in our reproductive center. Twenty-eight days after 3.75mg triptorelin was administrated on day 2 of her menstrual cycle, bilateral ovaries were significantly enlarged and presented multiple cystic masses. The hormone profile was as follows: E2＞4870pg/ml, P 13.19ng/ml, FSH 14IU/L, and LH 10.77IU/L. The patient felt symptoms of mild ovarian hyperstimulation syndrome. In the subsequent IVF treatment cycle, antagonist protocol was performed. It showed that follicles developed slowly and exogenous gonadotropins were used for 13 days. Finally, seven oocytes were obtained and only one blastocyst graded 4BC formed.

Conclusion:
Ovarian hyperstimulation following the sole administration of GnRHa can be occurred, but the mechanism is still not yet clear. Antagonist protocol may be an alternative fertility strategy, but the risk of poor embryo quality and low pregnancy rate of transplantation should be warned.