bluuebunny/crossref_metadata_2025_split huggingface dataset:
In vitro anti-proliferative activity of selected nutraceutical compounds in human cancer cell lines
Authors: Nibret Endalkachew , Krstin Sonja , Wink Michael 
Published: January 2021

Abstract
Objective
We investigated the anti-proliferative or cytotoxic activities of five nutraceutical compounds: allyl isothiocyanate, β-carotene, caffeine, capsaicin, and lupanine that we consume respectively, for example, from mustard seeds, carrot, coffee, pepper, and lupin seeds against cancer cell lines (human colon: HCT 116 p53 wild type, HCT 116 p53−/− and lymphoblastic: CEM/CCRF, CEM/ADR5000).

Result
Out of the five compounds tested in vitro, capsaicin and β-carotene were more cytotoxic than the other three compounds against the four cancer cell lines. The most potent nutraceutical compound was capsaicin and it exerted its highest cytotoxicity against HCT 116 p53−/− with IC50 value of 19.67 ± 0.06 µM. It is worth considering capsaicin for further development of anticancer drug against both colon and leukemia cancer types.
How to perform an excellent radiology board examination: a web-based checklist
Authors: Dicle Oğuz , Özan Sema , Şahin Hatice , Seçil Mustafa 
Published: January 2021

Abstract
Background
Board exams are now considered as means of quality procedures that aim to keep the professional knowledge and skills of the physicians at the highest level. In addition, for an assessment to be scientifically valid, it has to be done within defined standards. Although there are different sources in this field, there is a need for a resource that details the steps required for the examinations to be performed perfectly, brings descriptions of the reasons for the procedure and associates the steps with assessment standards. Experts with national and international experience both in radiology and medical education contributed to the preparation of this checklist.

Results
The guide includes 174 elements to consider before, after the exam order and examination. From the perspective of assessment standards, it has been observed that the steps to be considered before the exam have a greater impact on the validity and reliability of the exam. The standard in which the questions are most associated was validity with 117 (67.24%) questions.

Conclusions
We think that our guide, which will be accessible in the web environment, will be useful to the teams with a development goal or just start the exam, the candidates who will take the exam and the examiners.
Human embryonic stem cell-derived extracellular vesicles alleviate retinal degeneration by upregulating Oct4 to promote retinal Müller cell retrodifferentiation via HSP90
Authors: Ke Yifeng , Fan Xiaoe , Hao Rui , Dong Lijie , Xue Min , Tan Liangzhang , Yang Chunbo , Li Xiaorong , Ren Xinjun 
Published: January 2021

Abstract
Objective
Retinal degenerative diseases remain the dominant causes of blindness worldwide, and cell replacement is viewed as a promising therapeutic direction. However, the resources of seed cells are hard to obtain. To further explore this therapeutic approach, human embryonic stem extracellular vesicles (hESEVs) were extracted from human embryonic stem cells (hESCs) to inspect its effect and the possible mechanism on retinal Müller cells and retinal function.

Methods
hESEVs were extracted by multi-step differential centrifugation, whose morphologies and specific biomarkers (TSG101, CD9, CD63, and CD81) were observed and measured. After hESEVs were injected into the vitreous cavity of RCS rats, the retinal tissues and retinal functions of rats were assessed. The alteration of Müller cells and retinal progenitor cells was also recorded. Microvesicles (MVs) or exosomes (EXOs) were extracted from hESCs transfected with sh-HSP90 or pcDNA3.1-HSP9, and then incubated with Müller cells to measure the uptake of EVs, MVs, or EXOs in Müller cells by immunofluorescence. The retrodifferentiation of Müller cells was determined by measuring Vimentin and CHX10. qRT-PCR and western blot were used to detect HSP90 expression in MVs and evaluate Oct4 level in Müller cells, and Co-IP to inspect the interaction of HSP90 and Oct4.

Results
RCS rats at the postnatal 30 days had increased retinal progenitor cells which were dedifferentiated from Müller cells. hESEVs were successfully extracted from hESCs, evidenced by morphology observation and positive expressions of specific biomarkers (TSG101, CD9, CD63, and CD81). hESEVs promoted Müller cells dedifferentiated and retrodifferentiated into retinal progenitor cells evidenced by the existence of a large amount of CHX10-positive cells in the retinal inner layer of RCS rats in response to hESEV injection. The promotive role of hESEVs was exerted by MVs demonstrated by elevated fluorescence intensity of CHX10 and suppressed Vimentin fluorescence intensity in MVs rather than in EXOs. HSP90 in MVs inhibited the retrodifferentiation of Müller cells and suppressed the expression level of Oct4 in Müller cells. Co-IP revealed that HSP90 can target Oct4 in Müller cells.

Conclusion
hESEVs could promote the retrodifferentiation of Müller cells into retinal progenitor cells by regulating the expression of Oct4 in Müller cells by HSP90 mediation in MVs.
Characterisation of ovine bone marrow-derived stromal cells (oBMSC) and evaluation of chondrogenically induced micro-pellets for cartilage tissue repair in vivo
Authors: Futrega K. , Music E. , Robey P. G. , Gronthos S. , Crawford R. , Saifzadeh S. , Klein T. J. , Doran M. R. 
Published: January 2021

Abstract
Bone marrow stromal cells (BMSC) show promise in cartilage repair, and sheep are the most common large animal pre-clinical model.

Objective
The objective of this study was to characterise ovine BMSC (oBMSC) in vitro, and to evaluate the capacity of chondrogenic micro-pellets manufactured from oBMSC or ovine articular chondrocytes (oACh) to repair osteochondral defects in sheep.

Design
oBMSC were characterised for surface marker expression using flow cytometry and evaluated for tri-lineage differentiation capacity. oBMSC micro-pellets were manufactured in a microwell platform, and chondrogenesis was compared at 2%, 5%, and 20% O2. The capacity of cartilage micro-pellets manufactured from oBMSC or oACh to repair osteochondral defects in adult sheep was evaluated in an 8-week pilot study.

Results
Expanded oBMSC were positive for CD44 and CD146 and negative for CD45. The common adipogenic induction ingredient, 3-Isobutyl-1-methylxanthine (IBMX), was toxic to oBMSC, but adipogenesis could be restored by excluding IBMX from the medium. BMSC chondrogenesis was optimal in a 2% O2 atmosphere. Micro-pellets formed from oBMSC or oACh appeared morphologically similar, but hypertrophic genes were elevated in oBMSC micro-pellets. While oACh micro-pellets formed cartilage-like repair tissue in sheep, oBMSC micro-pellets did not.

Conclusion
The sensitivity of oBMSC, compared to human BMSC, to IBMX in standard adipogenic assays highlights species-associated differences. Micro-pellets manufactured from oACh were more effective than micro-pellets manufactured from oBMSC in the repair of osteochondral defects in sheep. While oBMSC can be driven to form cartilage-like tissue in vitro, the effective use of these cells in cartilage repair will depend on the successful mitigation of hypertrophy and tissue integration.
GABAB receptor antagonist promotes hippocampal neurogenesis and facilitates cognitive function recovery following acute cerebral ischemia in mice
Authors: Song Dan , Chen Yaohua , Chen Cheng , Chen Lili , Cheng Oumei 
Published: January 2021

Abstract
Purpose and background
Previous studies have suggested that promoting endogenous neurogenesis has great significance for the recovery of cognitive dysfunction caused by cerebral ischemia (CI). Pharmacological inhibition of GABAB receptor can enhance neurogenesis in adult healthy and depressed mice. In the study, we intended to investigate the effects of GABAB receptor antagonists on cognitive function and hippocampal neurogenesis in mice following CI.

Methods
Adult mice were subjected to bilateral common carotid artery occlusion (BCCAO) for 20 min to induce CI and treated with CGP52432 (antagonist of GABAB receptor, CGP, 10 mg/kg intraperitoneal injection) starting 24 h after CI. The Morris water maze test was performed to test spatial learning and memory at day 28. Immunofluorescence was applied to detect neurogenesis in the DG region at day 14 and 28. In in vitro experiments, cell proliferation was detected by CCK8 and immunofluorescence, and the expression of cAMP/CREB signaling pathway-related proteins was detected by ELISA assay and Western blot.

Results
CGP significantly improved spatial learning and memory disorders caused by CI, and it enhanced the proliferation of neural stem cells (NSCs), the number of immature neurons, and the differentiation from newborn cells to neurons. In vitro experiments further confirmed that CGP dose-dependently enhanced the cell viability of NSCs, and immunofluorescence staining showed that CGP promoted the proliferation of NSCs. In addition, treatment with CGP increased the expression of cAMP, PKA, and pCREB in cultured NSCs.

Conclusion
Inhibition of GABAB receptor can effectively promote hippocampal neurogenesis and improve spatial learning and memory in adult mice following CI.
Production of transglutaminase in glutathione-producing recombinant Saccharomyces cerevisiae
Authors: Hirono-Hara Yoko , Yui Miyuu , Hara Kiyotaka Y. 
Published: January 2021

AbstractTransglutaminase (TG) catalyzes the formation of cross-links between proteins. TG from Streptoverticillium mobaraense (SmTG) is used widely in food, cosmetic, biomaterial and medical industries. SmTG is occasionally supplied as a mixture with the activator peptide glutathione. Currently, glutathione is industrially produced using a budding yeast, Saccharomyces cerevisiae, because of its intracellular high content of glutathione. In this study, active SmTG was produced together with glutathione in S. cerevisiae. SmTG extracted from S. cerevisiae expressing SmTG showed cross-linking activity when BSA and sodium caseinate were substrates. The cross-linking activity of SmTG increased proportionally as the concentration of added glutathione increased. Furthermore, SmTG was prepared by extracting SmTG from an engineered S. cerevisiae whose glutathione synthetic pathway was enhanced. The SmTG solution showed higher activity when compared with a SmTG solution prepared from a S. cerevisiae strain without enhanced glutathione production. This result indicates that a high content of intracellular glutathione further enhances active SmTG production in S. cerevisiae. S. cerevisiae co-producing SmTG and a higher content of glutathione has the potential to supply a ready-to-use industrial active TG solution.
STAT3 plays an important role in DNA replication by turning on WDHD1
Authors: Zhou Yunying , Chen Jason J. 
Published: January 2021

Abstract
Background
Signal transducers and activators of transcription 3 (STAT3) is a transcription factor that plays a key role in many cellular processes such as cell growth and cancer. However, the functions and mechanisms by which STAT3 regulates cellular processes are not fully understood.

Results
Here we describe a novel function of STAT3. We demonstrated that STAT3 plays an important role in DNA replication. Specifically, knockdown of STAT3 reduced DNA replication while activation and ectopic expression of STAT3 promoted DNA replication. We further identified the WD repeat and HMG-box DNA-binding protein 1 (WDHD1), which plays an important role in DNA replication initiation, as a novel STAT3 target gene that mediated the DNA replication function of STAT3. We showed that STAT3 bind the promoter/up regulatory region of WDHD1 gene.

Conclusions
These studies identified a novel function of STAT3 that is mediated by its newly identified target gene WDHD1 and have important implications.
An efficient approach for solution of fractional-order Helmholtz equations
Authors: Shah Nehad Ali , El-Zahar Essam R. , Aljoufi Mona D. , Chung Jae Dong 
Published: January 2021

AbstractIn this article, a hybrid technique called the homotopy perturbation Elzaki transform method has been implemented to solve fractional-order Helmholtz equations. In the hybrid technique, the Elzaki transform method and the homotopy perturbation method are amalgamated. Three problems are solved to validate and demonstrate the efficacy of the present technique. It is also demonstrated that the results obtained from the suggested technique are in excellent agreement with the results by other techniques. It is shown that the proposed method is efficient, reliable and easy to implement for various related problems of science and engineering.
Stable bagging feature selection on medical data
Authors: Alelyani Salem 
Published: January 2021

AbstractIn the medical field, distinguishing genes that are relevant to a specific disease, let’s say colon cancer, is crucial to finding a cure and understanding its causes and subsequent complications. Usually, medical datasets are comprised of immensely complex dimensions with considerably small sample size. Thus, for domain experts, such as biologists, the task of identifying these genes have become a very challenging one, to say the least. Feature selection is a technique that aims to select these genes, or features in machine learning field with respect to the disease. However, learning from a medical dataset to identify relevant features suffers from thecurse-of-dimensionality. Due to a large number of features with a small sample size, the selection usually returns a different subset each time a new sample is introduced into the dataset. This selection instability is intrinsically related to data variance. We assume that reducing data variance improves selection stability. In this paper, we propose an ensemble approach based on the bagging technique to improve feature selection stability in medical datasets via data variance reduction. We conducted an experiment using four microarray datasets each of which suffers from high dimensionality and relatively small sample size. On each dataset, we applied five well-known feature selection algorithms to select varying number of features. The proposed technique shows a significant improvement in selection stability while at least maintaining the classification accuracy. The stability improvement ranges from 20 to 50 percent in all cases. This implies that the likelihood of selecting the same features increased 20 to 50 percent more. This is accompanied with the increase of classification accuracy in most cases, which signifies the stated results of stability.
The spectral scalings of magnetic fluctuations upstream and downstream of the Venusian bow shock
Authors: Xiao S. D. , Wu M. Y. , Wang G. Q. , Chen Y. Q. , Zhang T. L. 
Published: January 2021

AbstractWe statistically investigate the spectral scalings of magnetic fluctuations at the upstream and downstream regions near the Venusian bow shock and perform a differentiation by shock geometry. Based on the Venus Express data, 115 quasi-parallel ($$Q_{\parallel }$$

Q
‖

) bow shock crossings and 303 quasi-perpendicular ($$Q_{ \bot }$$

Q
⊥

) bow shock crossings are selected. The statistical results suggest that the bow shock tends to modify the upstream spectra flatter to 1/f noise in the magnetohydrodynamics (MHD) regime and steeper to turbulence in the kinetic regime after the magnetic fluctuations crossing the bow shock, and this modification for the $$Q_{\parallel }$$

Q
‖

and $$Q_{ \bot }$$

Q
⊥

bow shocks is basically consistent. However, the upstream spectral scalings are associated with the shock geometry. The changes of the spectral scalings of magnetic fluctuations near the $$Q_{\parallel }$$

Q
‖

bow shocks are not as significant as near the $$Q_{ \bot }$$

Q
⊥

bow shock crossings. That might result from the fluctuations generated by the backstreaming ions which can escape across the $$Q_{\parallel }$$

Q
‖

bow shock into the foreshock. Our results suggest that the energy cascade and dissipation near Venus can be modified by the Venusian bow shock, and the $$Q_{\parallel }$$

Q
‖

bow shock plays an important role on the energy injection and dissipation in the solar wind interaction with Venus. The large dispersion of spectral scalings indicates that this fluctuation environment is complicated, and the shock geometry is not the only key factor in the fluctuations across the Venusian bow shock. Other possible factors in the shock modification to the upstream fluctuations will be explored in future.
How can basic research on spatial cognition enhance the visual accessibility of architecture for people with low vision?
Authors: Creem-Regehr Sarah H. , Barhorst-Cates Erica M. , Tarampi Margaret R. , Rand Kristina M. , Legge Gordon E. 
Published: January 2021

AbstractPeople with visual impairment often rely on their residual vision when interacting with their spatial environments. The goal of visual accessibility is to design spaces that allow for safe travel for the large and growing population of people who have uncorrectable vision loss, enabling full participation in modern society. This paper defines the functional challenges in perception and spatial cognition with restricted visual information and reviews a body of empirical work on low vision perception of spaces on both local and global navigational scales. We evaluate how the results of this work can provide insights into the complex problem that architects face in the design of visually accessible spaces.
Assessing the acceptability, reliability, and validity of the EORTC Quality of Life Questionnaire (QLQ-C30) in Kenyan cancer patients: a cross-sectional study
Authors: Davda Jasmine , Kibet Hillary , Achieng Emmah , Atundo Lawrence , Komen Truphena 
Published: January 2021

Abstract
Background
In oncology practice, eliciting the patient’s perspective on their quality of life (QOL) adds important information and value to their treatment and care. The European Organization for Research and Treatment of Cancer QOL questionnaire (EORTC QLQ-C30) is the most commonly used tool for this purpose but has not been validated in Kenya. The present study aimed to conduct a preliminary assessment of the QOL among Kenyan cancer patients and examine the psychometric properties of the tool in this population.
One hundred patients with heterogeneous types of cancer were enrolled in this cross-sectional study between July and August 2019. The EORTC QLQ-C30 questionnaire was administered to patients using either the English or Kiswahili official version. Descriptive statistics were used to assess patient demographics and clinical characteristics. The psychometric properties of the EORTC QLQ-C30 were evaluated in terms of acceptability, internal consistency, and construct validity using statistical software packages, STATA and SPSS.

Results
The EORTC QLQ-C30 was found to be acceptable for use in our patient population as indicated by high compliance and low missing responses. Of the 100 patients, 66 were able to self-administer the questionnaire. The average time for completion was 13 min. Preliminary QOL assessment indicated an average QOL in Kenyan cancer patients (53 ± 27). Among the function scales, participants scored the lowest on the social function scale (51 ± 36) whereas among the symptom scales, participants scored the highest on the financial difficulties scale (79 ± 31). Cronbach’s alpha coefficient values ranged from 0.72–0.95, illustrating the reliability of the scales measured. Interscale correlations were statistically significant (p < 0.05), indicating clinical validity of the data collected. The magnitudes of the correlations between the physical functioning scale and the role functioning, pain, and fatigue scales were consistent with the values published in other studies across different geographical populations, further cross-validating the results from our study.

Conclusion
The results from this study provide important first insights into using EORTC QLQ-C30 in the Kenyan population. We conclude that the questionnaire is an acceptable, reliable, and valid instrument for measuring the QOL in cancer patients in Kenya and recommend its use in clinical practice.
Stature estimation from handprint measurements: an application to the medicolegal investigation
Authors: Asadujjaman Md. , Hossain Md. Golzer , Rana Md. Sohel , Islam Md. Zahidul 
Published: January 2021

Abstract
Background
The estimation of the stature of human beings is a major part of medicolegal investigation when only body parts are found. The study aimed to estimate the stature from different handprint measurements in a Bangladeshi population using statistical considerations. A sample of 200 young Bangladeshi adults (100 men and 100 women) with no physical disabilities participated in this study. Stature and seven anthropometric measurements were measured using standard anthropometric measurements. The bilateral asymmetry was tested using the independent t test. The Pearson’s correlation coefficient (R) between the stature and different handprint measurements was calculated. Consequently, the simple and multiple linear regression models were developed to estimate the stature from the handprint measurements.

Results
The bilateral asymmetry was statistically not significant (p > 0.05) in right and left handprints. Sex difference significantly (p < 0.05) influences the relationship between stature and handprint measurements. A positive and strong coefficient of correlation (R) value presents between stature and the handprint measurements. The right 2nd digit length in men (R = + 0.709, R2 = 0.502, SEE = ± 44.141 mm) and the right handprint length in women (R = + 0.552, R2 = 0.305, SEE = ± 49.074 mm) were the most reliable estimator of stature. However, when data were combined for both sexes, the right handprint length was identified as the most reliable estimator of stature with higher values of R (+ 0.777) and R2 (0.603), and a lower value of SEE (± 55.520). Multiple regression equation showed greater reliability than linear regression equations in stature estimation from handprint measurements in Bangladeshi population.

Conclusions
It was concluded that the estimation of stature from handprint measurements is possible and reliable. The findings of this study are very useful from the forensic and medicolegal point of view and can use to estimate the stature in Bangladeshi population.
The olfactory organ of schilbid catfish Eutropiichthys vacha (Hamilton, 1822): morphological and ultrastructural studies
Authors: Ghosh Saroj Kumar 
Published: January 2021

AbstractBackgroundA study of the olfactory organ structure in freshwater catfish,Eutropiichthys vacha, was carried out to explore the cellular constituents by aid of light as well as scanning and transmission electron microscopy.ResultsThe paired elongated olfactory organs were situated on the dorsolateral facet of the head in the mold of simple pits. The olfactory organ was made up of a series of leaflets, the lamellae, which embedded into both sideways of slender central raphe, forming a rosette distinguished with sensory and nonsensory areas. The sensory receptor cells were present on sideward surface and linguiform process of olfactory lamella while the rest of the portion of the lamella was lined with nonsensory epithelium. Olfactory cells were characterized by their staining intensity, outline, surface features, and comprehensive morphology in the epithelium. The sensory mucosa was defined by the occurrence of three types of neuron: classic types bearing either cilia or numerous microvilli and third type having rod-shaped architecture. The nonsensory epithelium was composed of mucous cells, labyrinth cells, mast cells, and two types of supporting cells categorized as ciliated or nonciliated. Basal cells lie deep in the olfactory lining, near the central core.ConclusionThe structural components of the olfactory apparatus crucial for olfaction were correlated with the behavioral activities of fish.