bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Comparative Study of Surface Hardness between Two Bioceramic Materials in an Experimental Apexification Model under Wet and Dry Conditions
Authors: Paul Maria Priya , Amin Swathi , Mayya Arun , Naik Rajaram , Mayya Shreemathi S. 
Published: January 2021

Background:The placement of a wet cotton pellet against Mineral Trioxide Aggregate (MTA) is often recommended to ensure the completion of its setting reaction.Objective:This study aimed to evaluate the setting behaviour of MTA Angelus and NeoMTA by comparing their hardness after placing them in dry and moist conditions.Methodology:A simulated open apex was created on 40 polyvinyl tubes. The apical 4 mm of the tubes was filled with the two materials, NeoMTA Plus (Avalon Biomed Inc. Bradenton, FL, USA) and MTA Angelus (Angelus, Londrina, PR, Brazil) (n=20 per group). Both groups were subdivided into two subgroups based on the dry and wet conditions (n=10 per group). A wet cotton pellet was placed above the two materials in the wet group, and the coronal segment was sealed using Type II Glass ionomer cement (GC corporation Tokyo, Japan). Gutta-percha was placed against the test materials in the dry group with the coronal segment sealed with amalgam. Samples were placed in an oasis soaked in phosphate-buffered saline for seven days in 100% humidity and at 37°C. Microhardness was measured independently at 4 and 2 mm from the apex. Hardness was compared between materials and conditions, applying analysis of variance (a = .05).Results:The presence of dry or wet conditions had no significant effect on material hardness. MTA Angelus showed significantly higher hardness values compared with NeoMTA Plus.Conclusion:The moisture of the periapical environment can compensate for the absence of a wet cotton pellet and is adequate for the setting of the materials.
Quantitative Detection of Periodontopathogenic Bacteria in Atherosclerotic Plaques from Coronary Arteries by Real-Time PCR
Authors: Sargolzaie Naser , Naghibi Nava , Khajavi Amin , Moeintaghavi Amir , Tashnizi Mohammad Abbasi , Ghazvini Kiarash , Shiezadeh Farid 
Published: January 2021

Objectives:Epidemiologic studies have suggested periodontitis as a risk factor for Coronary Artery Diseas (CAD). Detection of periopathogens in atheromatous plaque provides some evidence for the causal relationship between these two conditions. The aim of this study was to determine the presence and quantity of periopathogens in coronary atherosclerotic plaques in patients undergoing Coronary Artery Bypass Graft (CABG) surgery.Methods:20 patients who were candidates for endarterectomy were enrolled in this study for the periodontal examination. Subgingival and coronary atherosclerotic plaque samples were then collected. Thereafter, quantitative detection ofAggregatibacter actinomycetemcomitans (A.a), Porphyromonas gingivali (P.g), and all bacteria detected by Real-Time PCR (RT-PCR) were measured. The correlation analysis was also used to evaluate the relationship between quantities of periopathogens in atherosclerotic and subgingival plaque samples.Results:A.awas detected in 13 patients (65%) with subgingival plaques and 4 patients (20%) with atherosclerotic plaques. In addition,P.gwas found in 15 patients (75%) with subgingival and 10 patients (50%) with atherosclerotic plaques.A.arepresented means of 2.7% and 10.04% of detected bacteria in both atherosclerotic and subgingival plaque samples, respectively. The mean of quantity ofP.gwas 10.85% and 12.87% of the detected bacteria obtained from atherosclerotic and subginigival samples, respectively. Correlation analysis showed a significant correlation between the quantities ofA.ain the atherosclerotic and subgingival plaques, but such a significant relationship was not found forP.g.Conclusion:This study confirmed the detection ofA.aandP.gin atheromatous plaque. The quantitative data suggested that periopathogens comprise a significant proportion of atherosclerotic plaque microbiome, which may consequently contribute to the development of CAD.
Evaluation of the Relationship between Trait and State Anxiety and Temporomandibular Dysfunction
Authors: de Deus Lages Livio Portela , Guimarães Antonio Sérgio , Monte Tiago Lima , de Cássia Bergamaschi Cristiane , Ramacciato Juliana Cama , Lopes Motta Rogério Heládio 
Published: January 2021

Background: Anxiety is an important factor in the process of chronic pain in patients with Temporomandibular Dysfunction (TMD).

Objective:
The objective of this study was to evaluate the influence of minimally invasive management of muscular TMD on the levels of anxiety (state and trait), pain, and other parameters in patients with muscular TMD.

Methods:
A total of 83 patients (64 women and 19 men; mean age, 25.16 ± 4.40 years) who were clinically diagnosed with TMD were evaluated. During the first clinical session (T1) and after clinical evaluation and diagnosis, the participants filled out a pain visual analog scale and answered the Trait Anxiety Inventory (STAI-T) and State Anxiety Inventory (STAI-S). In addition, the bite strength of the participants was evaluated, and minimally invasive management (thermotherapy, orientation exercises, and cognitive behavioral therapy) was initiated in that session. After 8 weeks (T2), all parameters were reevaluated. The results obtained were statistically analyzed and found to have a significance level of 5%.

Results:
A significant decrease in pain, STAI-T, and STAI-S scales were observed from T1 to T2 (p < 0.0001). Higher values of bite strength were observed at T2 compared to T1 (p < 0.0001). Furthermore, a significant correlation was observed between the levels of pain, trait-anxiety, and state-anxiety.

Conclusion:
Minimally invasive management of TMD resulted in a reduction in the levels of pain and anxiety (trait and state) of the participants evaluated, suggesting a possible association of these factors with chronic myofascial pain.
Mineralized Plasmatic Matrix for Horizontal Ridge Augmentation in Anterior Maxilla with and without a Covering Collagen Membrane
Authors: Abdelfadil Ehab , Aboelmaaty Wael 
Published: January 2021

Background:Mineralized Plasmatic Matrix [MPM] is a unique form of platelet rich fibrin that contains mineralized bone graft particles within a fibrin network.Aim:This study was conducted to evaluate horizontal ridge augmentation using MPM with and without a coverage membrane.Materials and Methods:Sixteen edentulous spaces were randomly divided into 2 equal groups. MPM was used for horizontal ridge augmentation with and without a covering collagen membrane (group 1 and 2, respectively). Cone Beam CT images were obtained preoperatively as well as 1 week and 4 months postoperatively to evaluate alveolar ridge and the resorption of the grafting material at 3 predetermined points along with the site where the future dental implant will be placed.Student’s t-test (Unpaired) was used for comparing two different groups with quantitative parametric data and student’s t-test (Paired) was used for comparing two related groups with quantitative parametric data while repeated measures ANOVA (Analysis of variance) followed by post-hoc Bonferroni was used for comparing more than two related groups with quantitative parametric data.Student’s t-test (Unpaired) was used for comparing two different groups with quantitative parametric data and student’s t-test (Paired) was used for comparing two related groups with quantitative parametric data while repeated measures ANOVA (Analysis of variance) followed by post-hoc Bonferroni was used for comparing more than two related groups with quantitative parametric data.Results:There was no statistically significant difference between the gained bone width in both groups. More but not statistically significant resorption was recorded in group 2.Conclusion:MPM can be successfully used for horizontal ridge augmentation without a barrier membrane.
Ultrasound-guided Arthroscopic Tuberoplasty for Greater Tuberosity Malunion: A Case Report
Authors: Ando Akira , Suzuki Kazuaki , Koide Masashi , Hagiwara Yoshihiro 
Published: January 2021

Background:
Greater Tuberosity (GT) malunion can lead to impingement against the acromion, resulting in pain, stiffness, and weakness of the rotator cuff. For patients with lesser degrees of displacement, partial removal of the GT with rotator cuff repair (tuberoplasty) under fluoroscopic guidance is considered.

Case report:

A sixty-five year old female fell from a standing height and suffered a minimally displaced isolated GT fracture. She was conservatively managed for four months and referred to our institution due to persisting pain and stiffness. The shoulder motion was severely restricted (anterior elevation: 90°, lateral elevation: 45°, external rotation with the arm at side: 25°, hand behind back: 4th lumber vertebrae) and pain aggravated especially when laterally elevated. Plain radiography and computed tomography showed small superiorly malunited GT and magnetic resonance imaging showed no rotator cuff pathology. Ultrasound images showed impingement of the GT against the acromion when laterally elevated. Arthroscopic excision of the malunited GT and rotator cuff repair along with capsular release and acromioplasty was performed under ultrasound guidance. The ultrasound images were simultaneously delineated to the arthroscopic monitor. Dynamic evaluation of the reshaped GT passing under the acromion was possible.

Conclusion:
Intraoperative use of ultrasound during arthroscopic tuberoplasty offers advantages over fluoroscopic guidance concerning control of the amount of bone resection and dynamic evaluation between the GT and the acromion in addition to the problems of radiation and space-occupying devices.
Changes in Peripheral Blood Biomarkers with Aging and Neurodegenerative Disorders
Authors: Lima Marcela , Pestana Cezar 
Published: January 2021

Background:
Aging is associated with most neurodegenerative disorders such as Parkinson's
and Alzheimer's diseases. Determination of peripheral blood biomarkers represents a low
invasive approach for tracking early changes in body metabolism during aging.

Objectives:
This study addresses a cross-sectional analysis to identify changes in lipid, minerals, and
antioxidant capacity as potential biomarkers to the onset of neurodegenerative diseases during aging.

Methods:
A retrospective analysis was performed to determine age-related biomarkers from a clinical
sample database. Next, one hundred volunteers between 20-59 (adult) and over 60 years (elderly)
were selected for the motor and cognitive tests according to Unified Parkinson's Disease Rating
Scale (UPDRS) and Mini-Mental State Examination (MMSE), respectively. Peripheral blood samples
were also collected to determine circulating lipids, minerals, and antioxidant capacity.

Results:
Lipid profile revealed an increase in triglycerides, total, and VLDL Cholesterol. Among
the elderlies, HDL was lower than the adult group, particularly in volunteers with severe cognitive
decline. Minerals involved in antioxidant defense such as iron, selenium, and manganese were
lower in elderly people compared to adults. Catalase activity was also reduced among elderlies
with mild cognitive impairment.

Conclusion:
Here, we show changes in key serum biomarkers correlate with aging and clinical
cognitive decline among elderly people. These findings may contribute to the understanding of
how biomarkers can be useful for early diagnosis and treatment of aging-related diseases.
Balance Control and Muscles Activity of An Elderly Retired Man During Different Focus of Attention Instructions
Authors: Amini Amin , Shirvani Hossein , Bazgir Behzad 
Published: January 2021

Background:
Good balance and muscle activity are essential to do daily activities. Recent studies have focused on the relations between balance and attention patterns. The study of the balance and muscle activity of people in different patterns of attention can clarify the nature of the effect of attention instructions.

Purpose:
The present study aims to evaluate the changes in different focus of attention instructions and quality of balance control and muscle activity in the elderly retired.

Methods:
Thirty elderly retired men [mean age: 51.6 ± 6.4 years] were recruited for this study. Subjects were selected through the berg balance test. Balance control ability [Biodex Balance System] and muscle activity [electromyography system] were assessed in two conditions, internal attention and external attention task. The data obtained were analyzed using repeated-measures ANOVA at a significant level of P<0.05.

Results:
Body sway and muscle activity parameters were correlated with different patterns of attention. During external focus condition, [1] Center of Pressure [CoP] total two-leg balance, CoP medio-lateral and CoP anterior-posterior for external-focus were lowered [P<0.05] and [2] Tibialis anterior and soleus for the leg, biceps brachii and triceps brachii for the arm, sternocleidomastoid and semispinalis capitis for external-focus were lowered [P<0.05].

Conclusion:
Improved static balance responses and alterations in postural control were observed under external focus conditions. An external focus of attention may be the preferred method for facilitating balance control and muscle activation in an elderly retired man.
Effect of Ginger Extract on 4T1 Breast Cancer Cell Line in Balb/c Mouse
Authors: Gholizadeh Alireza Pooyae , Ebrahimi Asa , Rahaie Mahdi , Samiee Farzaneh 
Published: January 2021

Background:
By considering [6]-gingerol as an important polyphenol, ginger has been
related to cancer.

Objective:
The main objective of this study is to evaluate the ginger extract on the expressions of
four genes from different categories involved in breast cancer in Balb/C mice after 14 days.

Methods:
In terms of this research, ethanol extraction methods for extracting ginger rhizomes with
the goal of increasing [6]-gingerol were used. In this regard, a MTT test was accomplished for the
whole ginger extract to confirm its effect on the 4T1 breast cancer cell line (murine mammary carcinoma
cell). Afterward, the transplanted breast cancer Balb/C mouse was used to be treated with the
whole ginger extract (0.02 g/kg of mouse weight). The expressions of STAT3, β-catenin, P53,
APC, genes were evaluated, after 14-day of treatment.

Results:
The results showed that 70% ethanol and microwave extraction method can be considered
as the best method for extracting ginger extract. The expression of β-catenin has decreased in the
blood and liver of the treated group. Moreover, the expression of STAT3 oncogenes has significantly
decreased in the liver tissue. In addition, the expressions of APC suppressor genes have increased
in the blood and liver.

Conclusion:
In conclusion, treatment by 0.02 g/kg of mouse weight of ginger extract after 14 days
increased the expressions of APC as suppressor genes and a β-catenin oncogene in blood and liver
of the breast cancer group.
Did The Program Kampung Iklim Lead Farmers to Implement more Adaptation Strategies? Case Study of Rice Farmers in Sleman Regency
Authors: Priyanto Moh. Wahyudi , Mulyo Jangkung Handoyo , Irham Irham 
Published: January 2021

The negative impacts of climate change caused many losses for farmers. One solution to minimize losses is to implement a climate change adaptation strategy. The Indonesian government established a program called the Program Kampung Iklim/Proklim in 2012 which aims to increase awareness of climate change and the capacity to implement community adaptation strategies. This study aimed to determine the effect of the Proklim on the implementation of climate change adaptation strategy by farmers. The study was conducted in Proklim and non-Proklim locations. By implementing the simple random sampling method, it was obtained 112 samples divided into 56 farmers from each Proklim and non-Proklim location. The results showed that farming experience, land area, membership in farmers group, access to weather information, awareness towards climate change and perceptions towards climate change risks are associated with more practice of adaptation strategy. The results also showed that the implementation of adaptation strategy was influenced by the Proklim location, so that the farmers involved in the program locations applied more climate change adaptation strategy than the farmers in non-program locations. The authors recommend that Proklim needs to be applied in many locations, especially areas that are vulnerable to climate change, so farmers can improve the implementation of climate change adaptation strategies.
Bacterial Detection Causing Subclinical Mastitis on Etawah Crossbreed Goat in Kokap, Kulon Progo, Yogyakarta Province
Authors: Artdita Clara Ajeng , Andityas Morsid , Prihanani Nur Ika , Budiyanto Yohanes Wawan 
Published: January 2021

Resident in Kokap, Kulon Progo, Yogyakarta Province has another income sector, that is dairy goat (Etawah Crossbreed) farming and they have been joining into farm group, which is called Kelompok Ternak. There are many bacteria that causing dairy goat intra mamary infection, thus the milk production will be contaminated. Two hundred and four (204) milk samples from 103 lactation goat in four farm groups in Kokap, Kulon Progo, Yogyakarta is used in this study. Out of these, 21 samples (10,29%) were detected strong positive (++ and +++) for subclinical mastitis by using California Mastitis Test (CMT). Among this positive result, the next step was bacteria detection. This tests were held in Preclinic Laboratory Animal Health Study Program, Vocational School, UGM. The samples were inoculated in blood agar, then colony was identify by Gram staining. The next steph is biochemical tests using McConkey Agar (MCA) and eosin methylene blue (EMB) as a selective media for Gram negative bacteria, manitol salt agar (MSA) as selective media for Staphylococcus, sugar fermented broth (glucose, lactose, mannitol, maltose, and sacharose), Kigler iron agar (KIA), lysine iron agar (LIA), motility indole ornithin (MIO), and Simmon’s citrate. The result was Staphylococcus aureus (80%), Staphylococcus epidermidis (20%), Escherichia coli (10%), and Klebsiella pneumoniae (10%).
Karakter Morfologi Rambut Kelompok Cervidae Indonesia
Authors: Phadmacanty Ni Luh Putu Rischa 
Published: January 2021

Identification through animal hair character is one of a very important forensic tool given the high level of animal trade in Indonesia, one of which is the deer family (Cervidae). Indonesia has four species of Cervidae, there are Rusa timorensis (Javan deer), Rusa unicolor (Sambar deer), Muntiacus muntjak (Barking deer) and Axis kuhlii (Rusa Bawean). Until now, no information about the morphological character of Indonesian cervidae’s hair. In this study, we used 30 shaft/individual/species from Javan deer (8 individuals), Sambar deer (5 individuals), Barking deer (5 individuals) and Bawean deer (5 individual) from specimens collection of Museum Zoologicum Bogoriense (MZB) and field collections. Hairs were analyzed for macroscopic and microscopic, with several parameters of morphology, cuticular structure, medula, cross-section, and medullary index. The result showed that the special character of this family was filled lattice medulla structure and can be used for species identification.
GAMBARAN LEUKOSIT KUCING PENDERITA FELINE PANLEUKOPENIA
Authors: Purnamaningsih Hary , Indarjulianto Soedarmanto , Yanuartono Yanuartono , Nururrozi Alfarisa , Widiyono Irkham , Hayati Rusmi 
Published: January 2021

Salah satu penyakit pada kucing dengan morbiditas dan mortalitas tinggi adalah Feline Panleukopenia (FPL). Tujuan dari penelitian ini adalah mengetahui gambaran leukosit kucing penderita Feline Panleukopenia. Penelitian ini menggunakan 27 ekor kucing jantan dan betina berbagai umur yang didiagnosa FPL berdasar Feline Parvo Virus Ag test. Semua kucing diambil darah secara lege artis sebanyak 1 ml, diperiksa jumlah leukositnya, kemudian dianalisis secara diskriptif. Hasil penelitian menunjukkan bahwa FPL lebih banyak diderita kucing jantan (59,3 % ) dari pada betina (40,7%). Kejadian FPL lebih banyak diderita kucing umur ≤ 6 bulan, yaitu 21 ekor (77,8 %) dibanding umur > 6 bulan, yaitu 6 ekor (22,2 %). Sebanyak 19 ekor (70,4 %) FPL mempunyai jumlah total leukosit < 1.000 sel/mm3, 4 ekor (14,8 %) 1.000 – 2.500 sel/mm3 dan 4 ekor yang lain (14,8 %) > 2.500 sel/mm3. Berdasarkan hasil penelitian ini disimpulkan bahwa sebagian besar penderita Feline Panleukopenia mengalami penurunan leukosit berat dengan prognosis infausta, terutama pada kucing jantan dan umur muda.
Study of doking molecule flavonoid Coleus amboinicus in TGF-1b receptor and lowering MDA concentration on Cisplatin-induce Wistar Rats
Authors: Mubarokah Wida Wahidah , Solfaine Rondius , Muniroh Lailatul 
Published: January 2021

The aims of this study is to evaluate molecular docking of flavonoid Coleus amboinicus (CA) extracts in transforming growth factor-1b and lowering MDA concentration on cisplatin-induced in Wistar rat. Eighteen male Wistar rats (Rattus norvegicus), 3 months of age with a body weight (BW) of 150-200 g, were allocated into three groups, with six animals per group. The control group received aquadest (P0), the treatment group were treated with single doses of cisplatin (5 mg/kg bw., ip) (P1) and received 100 mg/kg bw of the CA extracts (P2) respectively for 7 days. Bloods collected for analysis of serum alkaline phospatase (AP), Blood Nitrogen Urea (BUN) and Malondialdehid (MDA) concentrations. The levels of Malondialdehid (MDA) concentrations were analysed by Avidin-Horseradish Peroxidase (HRP) Sandwich-ELISA. All groups were sacrified for histopathology. Coleus amboinicus extract significantly decreased the level of AP, BUN and MDA concentrations compared to the control group (p<0.05). The level of MDA could be detected by its level significantly decreased in CA treatment group (p<0.05). Coleus amboinicus (CA) extract has a flavonoid as a marker compound of CA extract has stronger bind to the TGF-β1receptor than its of 3WA_601 ligand in silico analyzed. In histopathological examination showed that cisplatin-induced could alter severe multifocal hemorrhage, interstitial congestion, cell inflammatory, acute glomerular and tubular injury with necrotic cells. Immunohistochemical staining labeled with TGF-1β monoclonal antibodies (Mab) showed marked expression of brownish color aggregates on the surface of tubular epithelial cells and around glomerular mesangial cells in the CA treatment group. This study was concluded that CA extract is inhibited renal tissue injuries by lowering MDA and increasing TGF-1b expression on cisplatin-induced rats. Flavonoid as marker of CA extract has stronger bind to TGF-1b receptor by in silico.