bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effect of Notch1 Signaling on Cellular Proliferation and Apoptosis of Human Laryngeal Carcinoma
Authors: Li Dawei , Zhang Yifei , Chen Penghui , Xie Jin , Xu Dan 
Published: November 2021

Abstract
The pathological processes of occurrence and development of malignancies include the excessive proliferation and apoptosis resistance of neoplastic cells. The study aims to identify the effects of Notch1 signaling on the proliferation and apoptosis of laryngeal cancer cells in hypoxic microenvironment. Notch1 and Ki-67 expression in laryngeal squamous cell carcinoma (LSCC) tissue samples were detected by immunohistochemistry. The apoptotic index (AI) of LSCC was evaluated by TUNEL method. In laryngeal cancer cells, small interfering RNA (siRNA) technology was to inhibit Notch1 expression. Meanwhile, Real-time PCR detected Notch1, Hes1 and Hey1 mRNA expression, and Western blot detected Notch1 and Notch1 intracellular domain (N1ICD) protein expression. Annexin V-FITC/propidium iodide staining and Cell Counting Kit‑8 methods measured cell apoptosis and proliferation, respectively. Notch1 expression was detected in 63.55％(68/107) of LSCC samples and was significantly related to the proliferation index (PI) (P < 0.05) and AI (P < 0.05) in LSCC tissues. Furthermore, it was confirmed that hypoxia could induce proliferation and inhibit apoptosis of laryngeal carcinoma cells (P < 0.05). Meanwhile, Notch1 expression and Notch1 signaling activity could be upregulated by hypoxia (P < 0.05). In contrast, suppression of Notch1 signaling activity in hypoxic neoplastic cells could obviously decrease cell proliferation and increase cell apoptosis (both P<0.05). Our study has demonstrated that hypoxia may promote cell proliferation and inhibit cell apoptosis of laryngeal carcinoma. Notch1 signalling may exert a pivotal role in regulating the proliferation and apoptosis resistance of laryngeal cancer cells under hypoxia.
Nurses’ experiences of the role of organizational and environmental factors in the development of love of the profession: a qualitative study
Authors: Adib-Hajbaghery Mohsen , Bolandian-Bafghi Shahnaz , Zandi Mitra 
Published: November 2021

Abstract
Background

Love of the profession has significant relationship with nurses’ job motivation and care quality. However, there is limited information about organizational and environmental factors affecting LOP among nurses.
Aim

This study aimed at exploring nurses’ experiences of the role of organizational and environmental factors in the development of Love of the profession.
Methods

This qualitative study was conducted in 2020–2021 using the conventional content analysis approach. Participants were Fifteen Iranian nurses with deep Love of the profession, nursing instructors, and nursing managers purposefully selected from different healthcare and academic settings in seven large cities of Iran. Data were gathered via semi-structured interviews and were analyzed via the conventional content analysis method proposed by Graneheim and Lundman.
Results

Organizational and environmental factors affecting the development of Love of the profession were grouped into three main categories, The social context of the profession (with two subcategories), family support (with two subcategories), and organizational health (with four subcategories). Subcategories were respectively historical context of the profession, the evolving context, emotional family support, instrumental family support, quality of interpersonal relationships in the organization, level of organizational justice, level of authority delegation to nurses, and level of organizational support.
Conclusions

Different organizational and environmental factors can affect nurses’ Love of the profession development. Improving public image of nursing, providing nurses with stronger support, improving organizational climate, and strengthening interpersonal relationships in healthcare settings are recommended to develop nurses’ Love of the profession.
Effect of Canagliflozin on Estimated Fluid Volumes in Patients with Heart Failure and type 2 Diabetes: A Post-hoc Analysis of the CANDLE Trial
Authors: Fujiki Shinya , Imai Takumi , Tanaka Atsushi , Shimabukuro Michio , Uehara Hiroki , Nakamura Ikuko , Matsunaga Kazuo , Suzuki Makoto , Kashimura Takeshi , Minamino Tohru , Inomata Takayuki , Node Koichi 
Published: November 2021

Abstract
Background:In patients with chronic heart failure (CHF) and type 2 diabetes (T2D), inhibition of the sodium-glucose cotransporter-2 (SGLT2) improves cardiorenal outcomes, but the effects of the SGLT2 inhibitor canagliflozin on body fluid volume and renal function remain to be clarified.Methods:This was a post-hoc analysis of 233 patients with CHF and T2D in the CANDLE Trial (UMIN000017669), an investigator-initiated, multi-center, randomized open-label trial that compared the effect of canagliflozin (100 mg, n=113) with glimepiride (starting dose: 0.5 mg, n=120) on changes in N-terminal pro-brain natriuretic peptide. The time courses of estimated plasma volume (ePV, calculated with the Straus formula), estimated extracellular volume (eEV, determined by the body surface area), and estimated glomerular filtration rate (eGFR, calculated with the modified Cockcroft formula) were compared between the canagliflozin and glimepiride groups at weeks 4, 12, and 24. Results:Reductions in ePV and eEV were observed only in the canagliflozin group until week 12 (change from baseline at week 12, ePV; -7.63%; 95% confidence interval [CI], -10.71 to -4.55%, p<0.001, eEV; -123.15 mL; 95% CI, -190.38 to -55.92 mL, p<0.001). Whilst ePV stopped falling after week 12, eEV continued to fall until week 24 ([change from baseline at week 24] – [change from baseline at week 12], ePV; 1.01%; 95%CI, -2.30 to 4.32%, p=0549, eEV; -125.15 mL; 95% CI, -184.35 to -65.95 mL, p<0.001). An initial significant reduction in eGFR was observed in the canagliflozin group (change from baseline at week 4, -4.18 mL/min/1.73 m2; 95% CI, -5.99 to -2.37 mL/min/1.73 m2, p<0.001), but after 4 weeks, eGFR stopped falling, and the difference between groups became insignificant (change from baseline at week 24, -1.27 mL/min/1.73 m2; 95% CI, -3.05 to 0.51 mL/min/1.73 m2, p=0.162, [change from baseline at week 24] – [change from baseline at week 12], 0.89 mL/min/1.73 m2; 95% CI, -0.74 to 2.51 mL/min/1.73 m2, p=0.284).Conclusions:Canagliflozin reduced ePV and eEV gradually, whilst glimepiride did not. Maintenance of a modest reduction in ePV by canagliflozin suggests appropriate intravascular volume reduction contributing to cardiorenal benefits in patients with CHF and T2D.Trial Registration: UMIN000017669
Autophagy Overactivity Regulate Terf1 and Terf2 in Positive and Negative-Telomerase Cancer Cell Lines
Authors: panahi mohammad , Samani Saeed , Mohajeri Nasrin , Bafroee Akram Sadat Tabatabee , Baesi Kazem , Abdoli Asghar 
Published: November 2021

Abstract
A recent suggestion for cancer therapy is targeting intracellular homeostatic signaling pathways like autophagy providing the balance between metabolism and cell cycling. Our study focused on investigating the relationship between autophagy activation by Beclin1 transfection and assessing Terf1 and Terf2 expression as shelterin proteins. The beclin1-containing plasmid was introduced to the U-2OS and Huh7 cell lines using Lipofectamine. The LC3-II as an intracellular autophagosomal marker was detected in transfected cells by flow cytometry. Also, the cells were treated with 3-methyladenine and metformin as autophagy inhibitors and inducers, respectively. Finally, the expression levels of Terf1 and Terf2 were analyzed by real-time PCR. Fluorescent images and flow cytometry results proved excellent GFP expression in the transfected cells. The results of real-time PCR demonstrated that autophagy induction by Beclin1 was increased Terf1 expression level in U-2OS cells up to 451%, while Huh7 cells suffered from the decreased expression of Terf1. Altogether, Terf2 expression was enhanced significantly in both cell lines after 48h treatment in comparison with 24h treatment. The obtained data provided that Beclin1-based activation of autophagy leads to overexpression of some protective shelterin proteins.
Query-Based Extractive Text Summarization Using Sense-Oriented Semantic Relatedness Measure
Authors: Rahman Nazreena , Borah Bhogeswar 
Published: November 2021

Abstract
This paper presents a query-based extractive text summarization method by using sense-oriented semantic relatedness measure. We have proposed a Word Sense Disambiguation (WSD) technique to find the exact sense of a word present in the sentence. It helps in extracting query relevance sentences while calculating the sense-oriented sentence semantic relatedness score between the query and input text sentence. The proposed method uses five unique features to make clusters of query-relevant sentences. A redundancy removal technique is also put forward to eliminate redundant sentences. We have evaluated our proposed WSD technique with other existing methods by using Senseval and SemEval datasets. Experimental evaluation and discussion signifies the better performance of proposed WSD method over current systems in terms of F-score. We compare our proposed query-based extractive text summarization method with other methods participated in Document Understanding Conference (DUC) and as well as with current methods. Evaluation and comparison state that the proposed query-based extractive text summarization method outperforms many existing methods. As an unsupervised learning algorithm, we obtained highest ROUGE (Recall-Oriented Understudy for Gisting Evaluation) score for all three DUC 2005, 2006 and 2007 datasets. Our proposed method is also quite comparable with other supervised learning based algorithms. We also observe that our query-based extractive text summarization method can recognize query relevance sentences which meet the query need.
Chloroform and Cognitive Function in the General Population of the US Elderly: A Cross-Sectional Study
Authors: Lee Sohyae , Min Jin-Young , Min Kyoung-Bok 
Published: November 2021

Abstract
Background

Chloroform is a water disinfection by-product associated with hepatic, renal and neurotoxicity. High concentrations of chloroform are known to cause central nervous system depression; however, the association between blood chloroform levels and cognitive function in the general elderly population is unknown. In this study, we investigated the association between blood chloroform levels and cognitive function in the elderly US population (n=782).
Methods

We analyzed blood chloroform levels and cognitive function, as measured by the digit symbol substitution test (DSST), from the 2011-2012 and 2013-2014 National Health and Nutrition Examination Surveys (NHANES) for participants aged 60 years and older. Blood chloroform levels were measured using capillary gas chromatography and mass spectrometry with selected-ion monitoring detection and istotope-dilution.
Results

After adjusting for all covariates, increases in log-transformed blood chloroform levels were significantly associated with decreased DSST scores (β = -1.56, SE = 0.57, p-value 0.010). Compared with individuals in the lowest quartile of blood chloroform, the regression coefficients were significantly lower among those in the second and third tertile (tertile 2 β = -3.00 (SE = 1.25, p-value 0.022); tertile 3 β = -4.05 (SE = 1.22, p-value 0.002)). After stratification by obesity status, increases in log-transformed chloroform levels showed borderline significant associations with decreased DSST scores among the obese (β = -1.71, SE = 0.85, p-value 0.052).
Conclusions

Our finding suggest a possible link between blood chloroform levels and cognitive function in the US elderly population.
Identification of Pathways and Key Genes in Carotid Atherosclerosis by Bioinformatics Analysis
Authors: Zhang Di , Jing Bei , Li Xin , Shi Huimei , Chang Shiquan , Chen Zhenni , Zheng Yachun , Pan Yuwei , Zhao Guoping 
Published: November 2021

Abstract
Purpose: Carotid atherosclerosis is a serious vascular disease, leading to various cerebrovascular diseases. Methods: Gene expression profile of GSE100927 was selected to conduct differentially expressed genes. Then we performed protein-protein interactions, Gene ontology, and Kyoto Encyclopedia of Genes and Genomes analysis. Then we used HUVEC, HAVSMC, and THP-1 induced macrophages cells to conduct experimental verification. The experimental groups were as follows: Control group, Solvent control group, and Palmitic acid group. We measured the levels of reactive oxygen species in three cells via flow cytometer or fluorescence microscope. Then we detected apoptosis of HUVEC cells and HAVSMC cells or observed nucleus of THP-1 induced macrophages cells. Results: We selected male carotid atherosclerosis, with 10 control samples and 21 atherosclerosis samples. The results of pathway enrichment showed that “Toll-like receptor signaling pathway” ranked first. We chose IL1β, CCL4, SPP1, CCL3, IRF5. MMP7 and MMP9 for experimental verification. Palmitic acid increased the reactive oxygen species levels and the apoptosis rates of HUVEC cells and HAVSMC cells while increasing the activity of THP-1 induced macrophages cells, and it cannot increase the level of reactive oxygen species, and shrink the nucleus. Palmitic acid increased mRNA levels of IL1β, CCL4, SPP1, CCL3, IRF5. MMP7 and MMP9 in HUVEC cells and THP-1 induced macrophages, and increased the mRNA levels of CCL4 and MMP9 in HAVSMC cells，not changed the mRNA level of IRF5. Conclusion: IL1β，CCL3, CCL4, SPP1, IRF5, MMP7, and MMP9 are significant markers of carotid atherosclerosis.
Weaving an Innovation Network from the Middle Out: the Case of the Renewable Energy Ecosystem
Authors: Zohar Tali , Parag Yael , Ayalon Ofira 
Published: November 2021

Abstract
BackgroundRenewable energy (RE) systems are becoming a central component of the clean energy transition and often seen as the way to combat climate change. Their establishment requires innovation, investments, and deployment policies for emerging technologies. Governments around the world are increasingly trying to create and support the energy-tech and climate-tech innovation ecosystems in their attempt to promote an innovation-supporting environment. However, energy innovation policies are often aligned with the dichotomous notion of technology-push and market-pull, and overlook the social, political, and cultural contexts in which RE innovative technologies are embedded, and the multiple actors and interactions that are associated with their development. By combining the Middle-Out Perspective (MOP) and innovation literature, this paper argues that an innovation ecosystem could be weaved from the middle-out.MethodsThis paper analyses the case of Israel’s RE innovation ecosystem creation through the lens of the MOP and applies a socio-technical interpretation to the push and pull terminology. Process tracing methodology was applied to trace a sequence of events to determines whether an actor was pushed or pulled, the formation of a network of actors, and its evolvement to an energy innovation ecosystem from the middle-out. Data for the analysis was collected from interviews, policy papers, media articles and Governmental decisions.ResultsThe analysis demonstrates how middle actors push the implementation of RE technologies in Israel, and at the same time pull decision-makers and other middle actors to promote the low carbon transition. The push and pull forces and the interactions between actors lead to the engagement of new stakeholders in the innovation network, the adoption of more ambitious RE targets and supporting policies, and to the creation of an effective RE innovation ecosystem.ConclusionsThis paper uses the MOP as an analytical framework and the push and pull terminology to demonstrate how a middle actor initiates and develops an actor-network by interacting with other actors. As this network broadens, it forms an effective innovation ecosystem. Network of actors have the potential to lead change, provide innovative ideas, initiate research, encourage investments, create employment possibilities, and promote regional sustainable development.
Exploring the role of mindful eating and self-compassion on eating behaviours and orthorexia in people following a plant-based diet
Authors: Kalika Eliza , Egan Helen , Mantzios Michael 
Published: November 2021

Abstract
Orthorexia nervosa (ON) is a new concept that is more prevalent in vegan populations. The aim of this study was to explore problematic eating behaviours in a vegan population, and to explore whether mindful eating and self-compassion have an impact on orthorexia nervosa. Three hundred thirteen individuals following a vegan diet completed scales in Orthorexia, Self-Compassion, Mindful, Emotional, External and Restraint Eating. The results indicated that individuals with high levels of orthorexia nervosa display low levels of self-compassion, and high levels of restrained eating. Moreover, the findings indicated that self-compassion, but not mindful eating, partially mediated the relationship between restrained eating and orthorexia nervosa. The present results contribute to a better understanding of orthorexic eating behaviours in a vegan population, and identifies the mediating capacity of self-compassion. Future directions are discussed.
Melatonin-mediated Calcineurin Inactivation Attenuates Prion Protein-induced Nf-Kb- Driven Pro-Inflammation Response
Authors: Hong Jeong-Min , Moon Ji-Hong , Seol Jae-Won , Park Sang-Youel 
Published: November 2021

Abstract
Background: Prion diseases are a group of prevalent and rapidly progressive neurodegenerative disorders that lead to chronic inflammation and neuronal cell death. Calcineurin and autophagy mediate prion-induced neurodegeneration, suggesting that inhibition of calcineurin and autophagy could be a target for therapy. Melatonin has been reported to exert neuroprotective effects against calcium-dependent neuronal cell death.Methods: Real-time quantitative PCR was used to detect mRNA levels of proinflammatory cytokines. Western blot was used to analysis p-nfkb, p-bcl10, calcineurin, prpc and autophagy flux pathway. Immunocytochemistry was used to analysis p-nfkb and calcineurin. Ca2+ levels were measured by fluo-4 using confocal microscope. Calcineurin activity was used to detect with calcineurin cellular activity assay kit. Transmission electron microscopy (TEM) was used to detect autophagy flux.Results: In the present study, we investigated whether melatonin attenuates prion peptide-mediated neuroinflammation and reduces calcineurin. We found that melatonin treatment inhibits prion protein-induced apoptosis. Melatonin inhibited calcium up-regulation and protected the cells against prion peptide‑induced neuron cell death by calcineurin inactivation. Furthermore, melatonin increased p62 protein levels and decrease LC3-II protein levels indicating autophagic flux inhibition and melatonin inhibited prion protein-induced neurotoxicity through autophagy flux inhibition.Conclusions: Taken together, our results illuminate that melatonin attenuated prion protein-induced neurinflammation through calcineurin inactivation and autophagic flux reduction, and also suggest that melatonin may provide effective strategy for therapy against neurodegenerative diseases, including prion diseases.
Primary COVID-19 vaccine cycle and booster doses efficacy: analysis of Italian nationwide vaccination campaign
Authors: Lippi Giuseppe , Mattiuzzi Camilla 
Published: November 2021

Abstract
We provide here an updated analysis of primary COVID-19 vaccination and vaccine booster doses efficacy, emerging from the ongoing Italian nationwide COVID-19 vaccination campaign. Primary COVID-19 vaccination efficacy was 76-92% within 6 months, decreasing to 34-80% after 6 months. Administration of vaccine booster doses decreased SARS-CoV-2 infections by 65%, COVID-19 related hospitalizations and deaths by 69% and 97% compared to vaccine efficacy after 6 months, but also decreased SARS-CoV-2 infections by 39% compared to vaccine efficacy within 6 months. These results suggest that COVID-19 vaccine booster doses are important for restoring vaccine efficacy and further limiting virus circulation.
Investigation of the Relation Between Epithelial Barrier Function and Autism Symptom Severity in Children with Autism Spectrum Disorder
Authors: Nalbant Kevser , Erden Semih , Yazar Abdullah , Kılınç İbrahim 
Published: November 2021

Abstract
Autism spectrum disorder (ASD) is a common neurodevelopmental disorder characterized by limitations in mutual communication and social interaction as well as restricted, repetitive patterns of behaviors, interests, or activities. A number of biological abnormalities have been reported in ASD. Therefore the possible role of biological abnormalities in etiopathogenesis arouses research interest in this area. This is a case-control design study evaluating epithelial barrier function by comparing serum concentrations of occludin and zonulin in children with ASD (n = 60) and controls (n = 30). The Childhood Autism Rating Scale (CARS) was used to evaluate autistic symptom levels of all children. Serum occludin and zonulin levels were analyzed using an enzyme-linked immunosorbent assay. Serum occludin was significantly lower in children with ASD than in control subjects. In children with ASD, a decrease in occludin level was significantly associated with the disorder symptom levels item mean score (CARS total scores). Our findings showed that children with ASD had alterations in epithelial barrier function compared to the control group. The investigation of the mechanism underlying the different levels of occludin between ASD and controls may be of importance in clarifying the etiopathogenesis of ASD, as well as its follow-up and treatment.
Relationship Between Induced Polarization Relaxation Time and Hydraulic Characteristics of Water-Bearing Sand
Authors: Ma Zhao , Nie Lichao , Deng Zhaoyang , Yin Xin , Shen Junfeng , Li Ningbo 
Published: November 2021

Abstract

Induced polarization method has become a popular method for evaluating formation permeability characteristics in recent years because of its sensitivity to water body and water-bearing pore structure. Especially, the induced polarization relaxation time can reflect the macroscopic characteristics of the pore structure of rock and soil. Therefore, in order to study the relationship between relaxation time and permeability, eight different sizes of quartz sand were used to simulate water-bearing sand layers under different working conditions, and the induced polarization experiment and Darcy seepage experiment were carried out on the same sand sample in this paper, respectively. The experimental results show that the relation time and the evolution of the permeability are closely correlated with the sizes of quartz sand. According to the experimental data, with the particle size of the quartz sand as the link, the power function equation is fitted to better describe the relationship between the permeability and the relation time. It is worth noting that the equations obtained are only empirical equations for quartz sand and are not suitable for general applications.