bluuebunny/crossref_metadata_2025_split huggingface dataset:
Towards Understanding the Nature of High Frequency Ultrasound Backscatter from Cells and Tissues: an Investigation of Backscatter Power Spectra from Different Concentrations of Cells of Different Sizes
Authors: Kolios Michael , Czarnota G. J. , Worthington A. E. , Giles A. , Tunis A. S. , Sherar Michael D. 
Published: May 2021

<p>During cell death a series of structural changes occur within the cell. We have shown that cell ensembles and tissues undergoing structural changes associated with various cell death pathways can be detected using high-frequency ultrasound. In our effort to better understand the nature of backscatter from collections of cells (which emulate tissues), we have collected raw RF backscatter data from cells of two different sizes in solutions for a series of concentrations or in pellet form. Human acute myeloid leukemia cells (AML-5, ~10µm in diameter) and transformed prostate cells (~25-30µm in diameter) were imaged either in suspension or in pellet form. Images and radiofreqeuncy data were acquired using a VS40B ultrasound instrument (VisualSonics Inc., Toronto, Ont) and 20MHz, 30MHz and 40MHz transducers with -6dB bandwidths approaching 100%. The cells were either imaged in degassed phosphate buffered solution in which their volumetric fraction increased from 0.0025% to 2%, or in pellet form by using a swinging bucket centrifuge. It was found that the backscatter power (as measured by the mid-band fit) increased by ~ 3 dB for both cell types in dilute solutions for which the volumetric concentration was doubled for a specific range of cell concentrations (which was dependent on cell size). In pellet form the backscatter power from the prostate cell pellets was ~12-14dB greater than the AML cell pellets. A comparison of the spectral slopes also strongly suggests a change in the scattering source contributions when the cells are in pellets: the spectral slope was negative for all concentrations for prostate cells imaged at 40MHz, but positive when measured in pellets. This is consistent an increased contribution to the backscatter of smaller sized scatterers (such as the cell nucleus) that manifests itself only when the cells are in pellets but not in solution. These data will be compared to theoretical predictions and their significance discussed. </p>
Is your library website enough?
Authors: Banerjee Sanjoy , McCarthy Graham , Wilson Sally 
Published: May 2021

<p>Increasingly students and researchers are accessing and using information in a wide variety of settings on an array of different devices. Libraries need to adapt and re-purpose their data and information so that it can be used by patrons where, when and how they want to use it. To meet this need the Ryerson Library has initiated several projects to expand access to its resources beyond the traditional confines of the building and the website. </p>
SQLite Page Caching Algorithm
Authors: Ma Jason V 
Published: May 2021

<p>[Introduction]: "SQLite is a database which can be easily embedded inside an application written in the C programming language. One common use is inside the Mozilla Firefox web browser. It supports asubset of the SQL language but was not intended to be a replacement for a multi-user database suchas MySQL, Oracle or Microsoft SQL Server.The entire database is integrated into one *.c file and the command shell for the command lineinterface is inside another file. Thus to embed the database a developer just has to copy and pastethe source code into their database. It also comes in another format with separate files for eachcomponent but is not recommended for use due to slower performance. SQLite's developers indicate there is a 5-10% performance improvement when using the amalgamation due to compileroptimizations for a single file.</p>
<p>SQLite supports connections via multiple threads, but it does not support intra-query parallelism.For example commercial databases can divide a large query so that it is run concurrently acrossseparate CPUs. SQLite has a very coarse locking level. Since the database is located inside one file,the entire file is locked for writing. Concurrent reads can still occur on the file. It does not supportfiner levels of locking such as table or row locks."</p>
Real Wage Chronologies
Authors: Christofides Louis , Peng Amy Chen 
Published: May 2021

<p>We process information in a large number of Canadian wage contracts, signed over a period of several decades, to generate the long-run history of the real wage for each bargaining pair. We term these hitherto unexamined histories ‘chronologies’. We are able to generate 1574 continuous real wage chronologies and we examine the evolution of the real wage in each case. We explore the influence of productivity growth, the labour relations record of the pair, the influence of industry and region as well as the initial wage on the growth of the real wage rate over the decades in the sample. We also consider the relation between the mean and variance of the real wage contained in these chronologies.</p>
<p>JEL Classification:E31, J41, J50</p>
<p><br></p>
<p><strong>Keywords</strong>: Wages, productivity, labour relations, compensating differentials, convergence</p>
Canada, Libraries and the Digital Economy
Authors: Middleton Catherine A. 
Published: May 2021

<p>Presented at the OLA Super Conference, Toronto February 1, 2014</p>
<p>What I will talk about:</p>
<p> • Digital economy strategies</p>
<p> • Canada’s digital economy – wired broadband networks – mobile phones and broadband – extending network availability, increasing quality </p>
<p>• Libraries as enablers of the digital economy</p>
Numerical Distribution Functions of Likelihood Ratio Tests for Cointegration
Authors: MacKinnon James G , Haug Alfred A , Michelis Leo 
Published: May 2021

<p>This paper employs response surface regressions based on simulation experiments to calculate asymptotic distribution functions for the Johansen-type likelihood ratio tests for cointegration. These are carried out in the context of the models recently proposed by Pesaran, Shin, and Smith (1997) that allow for the possibility of exogenous variables integrated of order one. The paper calculates critical values that are very much more accurate than those available previously. The principal contributions of the paper are a set of data files that contain estimated asymptotic quantiles obtained from response surface estimation and a computer program for utilizing them. This program, which is freely available via the Internet, can be used to calculate both asymptotic critical values and P values. </p>
Organisational Design and the (dis)Integration of Human Factors in Production System Development
Authors: Neumann Patrick , Winkel Jorgen 
Published: May 2021

<p>This paper describes and generalises experiences from two cases of production system development in the Swedish context. We have observed the successful application of ergonomics to be related to the lack of coordination of human factors across groups responsible for different stages of the production system design process. This problem is magnified by the tendency for ‘ergonomics’ to be the responsibility of Human Resources departments who are distanced from they design process, rather than engineering teams directly engaged in design. Available tools to improve the integration of ergonomics considerations into the design process are briefly presented and discussed.</p>
miR-224-5p Carried by Human Umbilical Cord Mesenchymal Stem Cells-Derived Exosomes Regulates Autophagy in Breast Cancer Cells via HOXA5
Authors: Wang Yichao , Wang Pan , Zhao Lei , Chen Xiaoying , Lin Zhu , Zhang Ling , Li Zhaoyun 
Published: May 2021

Objective: In this study, we focused on the potential mechanism of miRNAs carried by human umbilical cord mesenchymal stem cells-derived exosomes (hUCMSCs-exo) in breast cancer (BC).Methods: RT-qPCR was conducted for the expression of miR-224-5p and HOXA5 in tissues and cells. After co-culture of exosomes and MCF-7 or MDA-MB-231 cells, the cell proliferation was observed by MTT and cell colony formation assay, while apoptosis was measured by flow cytometry. In addition, the expression of HOXA5 and autophagy pathway-related proteins LC3-II, Beclin-1 and P62 was detected by western blotting. And immunofluorescence was applied for detection of LC3 spots. The binding of miR-224-5p to HOXA5 was verified by the luciferase reporter gene assay and RNA-binding protein immunoprecipitation assay. Finally, in vivo experiment was performed to investigate the effect of miR-224-5p on BC growth.Results: MiR-224-5p was up-regulated and HOXA5 was down-regulated in BC tissues and cells. HOXA5 was confirmed to be the target gene of miR-224-5p. MiR-224-5p carried by hUCMSCs-exo was able to promote the proliferation and autophagy of BC cells, while inhibited apoptosis. Bases on xenograft models in nude mice, it was also revealed that miR-224-5p carried by hUCMSCs-exo could regulate autophagy and contribute to the occurrence and development of BC in vivo.Conclusion: MiR-224-5p carried by hUCMSCs-exo can regulate autophagy via inhibition of HOXA5, thus affecting the proliferation and apoptosis of BC cells.
Association Between Anticoagulation Outcomes and Venous Thromboembolism History in Chronic Thromboembolic Pulmonary Hypertension
Authors: Zhu Yong-Jian , Zhou Yu-Ping , Wei Yun-Peng , Xu Xi-Qi , Yan Xin-Xin , Liu Chao , Zhu Xi-Jie , Liu Zi-Yi , Sun Kai , Hua Lu , Jiang Xin , Jing Zhi-Cheng 
Published: May 2021

Background: The association between anticoagulation outcomes and prior history of venous thromboembolism (VTE) in chronic thromboembolic pulmonary hypertension (CTEPH) has not been established. This study aimed to compare the efficacy and safety of anticoagulation treatment in CTEPH patients with and without prior history of VTE.Methods: A total of 333 CTEPH patients prescribed anticoagulants were retrospectively included from May 2013 to April 2019. The clinical characteristics were collected at their first admission. Incidental recurrent VTE and clinically relevant bleeding were recorded during follow-up. The Cox proportional regression models were used to identify potential factors associated with recurrent VTE and clinically relevant bleeding.Results: Seventy patients (21%) without a prior history of VTE did not experience recurrent VTE during anticoagulation. Compared to CTEPH patients without a prior history of VTE, those with a prior history of VTE had an increased risk of recurrent VTE [2.27/100 person-year vs. 0/100 person-year; hazard ratio (HR), 8.92; 95% confidence interval (CI), 1.18–1142.00; P = 0.029] but a similar risk of clinically relevant bleeding (3.90/100 person-year vs. 4.59/100 person-year; HR, 0.83; 95% CI, 0.38–1.78; P = 0.623). Multivariate Cox analyses suggested that a prior history of VTE and interruption of anticoagulation treatments were significantly associated with an increased risk of recurrent VTE, while anemia and glucocorticoid use were significantly associated with a higher risk of clinically relevant bleeding.Conclusions: This study is the first to reveal that a prior history of VTE significantly increases the risk of recurrent VTE in CTEPH patients during anticoagulation treatment. This finding should be further evaluated in prospective studies.
Screening for Mutations in Isolated Central Hypothyroidism Reveals a Novel Mutation in Insulin Receptor Substrate 4
Authors: Patyra Konrad , Makkonen Kristiina , Haanpää Maria , Karppinen Sinikka , Viikari Liisa , Toppari Jorma , Reeve Mary Pat , Kero Jukka 
Published: May 2021

BackgroundCentral hypothyroidism (CeH) is a rare condition affecting approximately 1:16 000- 100 000 individuals. Congenital forms can harm normal development if not detected and treated promptly. Clinical and biochemical diagnosis, especially of isolated CeH, can be challenging. Cases are not usually detected in neonatal screening, which, in most countries, is focused on detection of the more prevalent primary hypothyroidism. Until now, five genetic causes for isolated CeH have been identified. Here we aimed to identify the genetic cause in two brothers with impaired growth diagnosed with CeH at the age of 5 years. We further evaluated the candidate gene variants in a large genetic database.MethodsClinical and biochemical characterization together with targeted next-generation sequencing (NGS) was used to identify the genetic cause in a family of two brothers presenting with CeH. Screening of insulin receptor substrate 4 (IRS4) variants was carried out in the FinnGen database.ResultsA novel monoallelic frameshift mutation c.1712_1713insT, p.Gly572Trp fs*32 in the X-linked IRS4 gene was identified by NGS analysis in both affected males and confirmed using Sanger sequencing. Their mother was an unaffected carrier. In addition to the declined growth at presentation, central hypothyroidism and blunted TRH test, no other phenotypic alterations were found. Diagnostic tests included head MRI, thyroid imaging, bone age, and laboratory tests for thyroid autoantibodies, glucose, insulin and glycosylated hemoglobin levels. Examination of the IRS4 locus in FinnGen (R5) database revealed the strongest associations to a rare Finnish haplotype associated with thyroid disorders (p = 1.3e-7) and hypothyroidism (p = 8.3e-7).ConclusionsHere, we identified a novel frameshift mutation in an X-linked IRS4 gene in two brothers with isolated CeH. Furthermore, we demonstrate an association of IRS4 gene locus to a general thyroid disease risk in the FinnGen database. Our findings confirm the role of IRS4 in isolated central hypothyroidism.
Efficacy of Electroacupuncture Therapy in Patients With Postherpetic Neuralgia: Study Protocol for a Multicentre, Randomized, Controlled, Assessor-Blinded Trial
Authors: Hu Hantong , Shen Yejing , Li Xinwei , Tian Hongfang , Li XingLing , Li Yang , Cheng Yingying , Wu Lei , Han Dexiong 
Published: May 2021

Introduction: The efficacy of conventional treatments for treating postherpetic neuralgia (PHN) remains unsatisfactory. Thus, this multicentre, randomized controlled, assessor-blinded trial aims to investigate the efficacy and safety of electroacupuncture (EA) therapy in patients with PHN.Methods and Analysis: This multicentre randomized controlled trial will enroll 132 patients with PHN from 3 hospitals. All patients will be randomly assigned to either the EA combined with medication group or medication group through a computerized central randomization system in a 1:1 ratio. Outcome measures will be assessed before intervention, at 2, 4, 6 weeks after intervention and at the end of 8-week follow-up. Primary outcomes will be sensory thresholds and pain intensity. Secondary outcomes will include dosage of analgetic, quality of life, anxiety, and depression severity and sleep quality. All adverse effects will be assessed during the trial.Conclusions: This study will provide evidence to ascertain whether EA is effective and safe for treating PHN.Ethics and Dissemination: Ethics approval (No.ZSLL-KY-2017-025) has been obtained from the Ethics Committee of The Third Affiliated Hospital of Zhejiang Chinese Medical University. Informed consent will be signed prior to subject enrolment. The results will be submitted to international peer-reviewed journals and presented at international conferences.Trial Registration Number: The study protocol has been registered in the clinicaltrials registry with the identification code NCT04594226.
Publication Trends of Research on Retinoblastoma During 2001–2021: A 20-Year Bibliometric Analysis
Authors: Gu Xiang , Xie Minyue , Jia Renbing , Ge Shengfang 
Published: May 2021

Background: Retinoblastoma is the most common primary intraocular malignancy of childhood. Despite high survival and eye salvage as the result of various types of therapies, retinoblastoma remains a disease that places a considerable burden on developing countries. Our study attempted to analyse the research trends in retinoblastoma research and compare contributions from different countries, institutions, journals, and authors.Methods: We extracted all publications concerning retinoblastoma from 2001 to 2021 from the Web of Science database. Microsoft Excel and VOSviewer were employed to collect publication data, analyse publication trends, and visualize relevant results.Results: A total of 1,675 publications with 30,148 citations were identified. The United States contributed the most publications (643) and citations (16,931 times) with the highest H-index value (67) as of February 4, 2021. China ranked second in the number of publications (259), while ranking fourth in both citations (2,632 times) and the H-index (26) ranked fourth. The British Journal of Ophthalmology was the most productive journal concerning retinoblastoma, and Abramson DH had published the most papers in the field. Keywords were categorized into three clusters; tumor-related research, clinical research, and management-related research. The keywords “intravitreal,” “intraarterial,” and “intravenous” appeared the most frequently, with the average appearing year being 2018.1, 2017.7, and 2017.1, respectively. Management-related research has been recognized as a heavily researched topic in the field.Conclusion: We conclude that the United States, China, and India made the most exceptional contributions in the field of retinoblastoma research, while China still has a disparity between the quantity and quality of publications. Management-related research, including intravitreal, intraarterial, and intravenous chemotherapy was considered as a potential focus for future research.
A Comparison of Safety and Effectiveness Between Wingspan and Neuroform Stents in Patients With Middle Cerebral Artery Stenosis
Authors: Zhou Kai , Cao Yuan , He Xiao-Hui , Qiu Zhong-Ming , Liu Shuai , Gong Zi-Li , Shuai Jie , Yang Qing-Wu 
Published: May 2021

Background: Percutaneous transluminal angioplasty and stenting with the Wingspan stent has proven safe and effective in patients with middle cerebral artery stenosis (MCAS), but the off-label use of the Neuroform stent might be an alternative treatment. This study aimed to compare the safety and effectiveness of the above two intracranial stents in patients with MCAS.Methods: We retrospectively analyzed consecutive patients with symptomatic MCAS who had been treated with the Neuroform EZ or the Wingspan stent. A propensity score was generated to control for differences in baseline characteristics. The endpoints were the rate of peri-procedural complications within 30 days after stenting, the in-stent restenosis rate, and any target-vessel-related stroke or deaths during follow-up.Results: After matching for propensity score, the peri-procedural complication rate in the Wingspan group was 7.4% compared with 5.6% in the Neuroform group (p = 1.00), while the follow-up in-stent restenosis rates were 23.3 vs. 14.3%, respectively (p = 0.41). In the restenosis group, the patients tended to be younger (p &lt; 0.01) and the degree of artery stenosis before stenting was higher (p &lt; 0.01).Conclusion: This study indicated that in patients with symptomatic MCAS, Neuroform EZ stents are an alternative to Wingspan. Moreover, younger age and higher degree of artery stenosis before stenting might be a risk factor of in-stent restenosis.
Angiotensin-(1-7) Central Mechanisms After ICV Infusion in Hypertensive Transgenic (mRen2)27 Rats
Authors: Kangussu Lucas M. , Melo-Braga Marcella Nunes , de Souza Lima Bruna Soares , Santos Robson A. S. , de Andrade Hélida Monteiro , Campagnole-Santos Maria José 
Published: May 2021

Graphical AbstractHypothalamic mechanisms induced by ICV infusion of angiotensin-(1-7) in the transgenic (mRen2)27 hypertensive animals. Ang-(1-7) treatment modulated components of the renin-angiotensin system (RAS), attenuating increased levels of AT1 and ACE activity, and negatively modulated inflammatory profile, by increasing anti-inflammatory IL-10 and decreasing TNFα and iNOS in the hypothalamus. Further, altered oxidative stress/inflammation by ROS mediator, decreasing Uchl1 (ubiquitin carboxyl-terminal hydrolase isozyme L1) while increasing antioxidant peroxiredoxin 1 (figure was created with pictures from biorender.com).
A Dynamic Transcription Factor Signature Along the Colorectal Adenoma-Carcinoma Sequence in Patients With Co-Occurrent Adenoma and Carcinoma
Authors: Pan Zongfu , He Ying , Zhu Wenjuan , Xu Tong , Hu Xiaoping , Huang Ping 
Published: May 2021

BackgroundColorectal carcinoma (CRC) often arises from benign adenoma after a stepwise accumulation of genetic alterations. Here, we profiled the dynamic landscapes of transcription factors (TFs) in the mucosa-adenoma-carcinoma progression sequence.MethodsThe transcriptome data of co-occurrent adenoma, carcinoma, and normal mucosa samples were obtained from GSE117606. Identification of differentially expressed TFs (DE-TFs) and subsequent function annotation were conducted in R software. Expression patterns of DE-TFs were clustered by Short Time-series Expression Miner software. Thereafter, modular co-expression analysis, Kaplan-Meier survival analysis, mutation profiling, and gene set enrichment analysis were conducted to investigate TF dynamics in colorectal tumorigenesis. Finally, tissue microarrays, including 51 tumors, 32 adenomas, and 53 normal tissues, were employed to examine the expression of significant candidates by immunohistochemistry staining.ResultsCompared to normal tissues, 20 (in adenoma samples) and 29 (in tumor samples) DE-TFs were identified. During the disease course, 28 expression patterns for DE-TFs and four co-expression modules were clustered. Notably, six DE-TFs, DACH1, GTF2IRD1, MEIS2, NR3C2, SOX9, and SPIB, were identified as having a dynamic signature along the colorectal adenoma-carcinoma sequence. The dynamic signature was of significance in GO enrichment, prognosis, and co-expression analysis. Among the 6-TF signature, the roles of GTF2IRD1, SPIB and NR3C2 in CRC progression are unclear. Immunohistochemistry validation showed that GTF2IRD1 enhanced significantly throughout the mucosa-adenoma-carcinoma sequence, while SPIB and NR3C2 kept decreasing in stroma during the disease course.ConclusionsOur study provided a dynamic 6-TF signature throughout the course of colorectal mucosa-adenoma-carcinoma. These findings deepened the understanding of colorectal cancer pathogenesis.