bluuebunny/crossref_metadata_2025_split huggingface dataset:
Performance of an automated solid-phase red cell adherence system compared with that of a manual gel microcolumn assay for the identification of antibodies eluted from red blood cells
Authors: Finck R.H. , Davis R.J. , Teng S. , Goldfinger D. , Ziman A.F. , Lu Q. , Yuan S. 
Published: May 2021

Abstract
IgG antibodies coating red blood cells (RBCs) can be removed by elution procedures and their specificity determined by antibody identification studies. Although such testing is traditionally performed using the tube agglutination assay, prior studies have shown that the gel microcolumn (GMC) assay may also be used with comparable results. The purpose of this study was to compare an automated solid-phase red cell adherence (SPRCA) system with a GMC assay for the detection of antibodies eluted from RBCs. Acid eluates from 51 peripheral blood (PB) and 7 cord blood (CB) samples were evaluated by both an automated SPRCA instrument and a manual GMC assay. The concordance rate between the two systems for peripheral RBC samples was 88.2 percent (45 of 51), including cases with alloantibodies (n = 8), warm autoantibodies (n = 12), antibodies with no identifiable specificity (n = 2), and negative results (n = 23). There were six discordant cases, of which four had alloantibodies (including anti-Jka, -E, and -e) demonstrable by the SPRCA system only. In the remaining 2 cases, anti-Fya and antibodies with no identifiable specificity were demonstrable by the GMC assay only. All seven CB specimens produced concordant results, showing anti-A (n = 3), -B (n = 1), maternal anti-Jk a (n = 2), or a negative result (n = 1). Automated SPRCA technology has a performance that is comparable with that of a manual GMC assay for identifying antibodies eluted from PB and CB RBCs.
Immunohematology

2011;27:1–5.
Weak D type 42 cases found in individuals of European descent
Authors: St-Louis M. , Richard M. , Côté M. , Éthier C. , Long A. 
Published: May 2021

Abstract
Patient samples were referred to our immunohematology reference laboratory to investigate the presence of a weak D antigen. In the last 3 years, 26 samples were received. Serology and molecular analyses were performed to identify the weak D variant. RHD mRNA from all patients was reverse transcribed, and cDNA was sequenced. The results were compared with a normal RHD sequence to identify the polymorphisms causing the weak D phenotype. Five different already known RHD variants were observed: weak D type 1 (5 individuals), weak D type 2 (1 individual), weak D type 42 (17 individuals), weak D type 45 (1 individual), and partial D DNB (2 individuals). Surprisingly, weak D type 42 was prevalent in our population, whereas weak D type 1, 2, and 3 are the most prevalent variants elsewhere. Anti-D was found in six cases of weak D type 42. The higher prevalence of weak D type 42 could be the result of a founder effect. Additional studies are needed to estimate the frequency of this variant in the general population.
Immunohematology

2011;27:20–24.
Preoperative coagulation studies to predict blood component usage in coronary artery bypass graft surgery
Authors: Josefy S. , Briones R. , Bryant B.J. 
Published: May 2021

Abstract
Bleeding remains a serious complication of cardiac surgery. Studies indicate that preoperative fibrinogen concentration is an independent predictor of blood loss during coronary artery bypass graft (CABG) surgery. This study evaluates whether fibrinogen concentration is a better predictor of blood usage than the prothrombin time (PT) and activated partial thromboplastin time (aPTT) tests. Patients not taking clopidogrel bisulfate who underwent CABG surgery during a 3-month period at a 350-bed community hospital were included in this prospective study. The parameters evaluated included patient’s age, preoperative coagulation test results (PT, international normalized ratio [INR], aPTT, fibrinogen), and number of blood components transfused. A probability value of less than 0.05 was deemed significant. Thirty-five patients were included in this study. Mean blood usage was 6 units. Patient’s age approached significance as a predictor of blood usage, and fibrinogen levels trended toward significance more than the other coagulation parameters. In this study, the increased age of the patient and low plasma concentrations of fibrinogen were associated with increased blood usage. Although no indicators clearly demonstrated statistical significance, the vast difference in the probability values for patients’ ages and fibrinogen levels indicated that there was a trend toward significance in blood usage for CABG patients. Further studies with larger patient populations are indicated.
Immunohematology

2011;27:151–153.
Granulocyte serology: current concepts and clinical signifcance
Authors: Clay M.E. , Schuller R.M. , Bachowski G.J. 
Published: May 2021

Abstract
Applying serologic procedures to the detection of RBC and lymphocyte antigens has facilitated the identification of granulocyte antigens with established clinical significance, which are now classified in the human neutrophil antigen system. Granulocyte alloantibodies and autoantibodies have been implicated in a variety of clinical conditions including alloimmune neutropenia, autoimmune neutropenia, febrile and severe pulmonary transfusion reactions, drug-induced neutropenia, refractoriness to granulocyte transfusions, and immune neutropenia after hematopoietic stem cell transplantation. Although the intrinsically fragile nature of granulocytes contributes to the inherent challenges of granulocyte serology, several advances in laboratory procedures have improved detection of granulocyte antibodies. This review will provide a current perspective about the importance and use of granulocyte serology for detection of granulocyte antibodies that have significant medical effects.
Immunohematology

2010;26:11–21.
The Duffy blood group system: a review
Authors: Meny G.M. 
Published: May 2021

Abstract
Duffy was the fi rst blood group mapped to an autosome (chromosome 1) using cytogenetic studies. Duffy antigens are located on a glycoprotein that can be found on erythrocytes and other cells throughout the body. Fya and Fyb are products of their respective alleles (FY*A, FY*B ). Fyx, characterized by weak Fyb expression, is a result of an additional mutation in FY*B. The Fy(a–b–) phenotype, most commonly found in Blacks, occurs primarily as a result of a GATA promoter region mutation upstream of the FY allele. This mutation prevents expression of Duffy glycoprotein on erythrocytes only, while permitting expression on nonerythroid cells. Other antigens include Fy3, Fy5, and Fy6. Antibodies to Duffy antigens are usually clinically signifi cant and have been reported to cause hemolytic disease of the fetus and newborn. This review provides a general overview of the Duffy blood group system, including the role of the Duffy glycoprotein as a chemokine receptor (Duffy antigen receptor for chemokines) and in malarial infection.
Immunohematology 2010;26:51–56.
The Gerbich blood group system: a review
Authors: Walker P.S. , Reid M.E. 
Published: May 2021

Abstract
Antigens in the Gerbich blood group system are expressed on glycophorin C (GPC) and glycophorin D (GPD), which are both encoded by a single gene, GYPC. The GYPC gene is located on the long arm of chromosome 2, and Gerbich antigens are inherited as autosomal dominant traits. There are 11 antigens in the Gerbich blood group system, six of high prevalence (Ge2, Ge3, Ge4, GEPL [Ge10*], GEAT [Ge11*], GETI [Ge12*]) and five of low prevalence (Wb [Ge5], Lsa [Ge6], Ana [Ge7], Dha [Ge8], GEIS [Ge9]). GPC and GPD interact with protein 4.1R, contributing stability to the RBC membrane. Reduced levels of GPC and GPD are associated with hereditary elliptocytosis, and Gerbich antigens act as receptors for the malarial parasite Plasmodium falciparum. Anti-Ge2 and anti-Ge3 have caused hemolytic transfusion reactions, and anti-Ge3 has produced hemolytic disease of the fetus and newborn (HDFN).
Immunohematology

2010;26:60–65.
Allogeneic adsorptions: a comparison of the traditional method with a modified PEG adsorption method
Authors: Etem M.E. , Laird-Fryer B. , Holub M.P. , Hedl J.J. , Symington D.B. , Figueroa D. 
Published: May 2021

Abstract
The purpose of this study is to demonstrate the benefits of enhancing adsorptions with PEG. Allogeneic adsorptions were performed on 20 patient samples containing warm reactive autoantibodies with two volumes of adsorbing RBCs; results using unenhanced adsorptions were compared with those using PEG-enhanced adsorptions and with using untreated adsorbing RBCs and ficin-treated adsorbing RBCs. Two volumes of adsorbing RBCs, one volume of serum, and one volume of PEG were used. The number of adsorptions, average time saved, and presence or absence of underlying alloantibodies were compared for the two methods and types of adsorbing RBCs. Modified PEG-enhanced adsorptions resulted in a 69 percent decrease in adsorbing time. PEG adsorptions removed all autoantibodies and detected 18 of 19 underlying alloantibodies. The unenhanced method did not remove autoantibodies in two samples and identified only 15 of 19 underlying alloantibodies. As expected, reductions in the number of adsorptions and adsorbing time were observed. The modified-PEG adsorption is an improved method that may replace the current unenhanced method.
Immunohematology

2010;26:104–108.
‘When you preach water and you drink wine’: WASH in healthcare facilities in Kenya
Authors: Abu Thelma Zulfawu , Elliott Susan J. , Karanja Diana 
Published: May 2021

Abstract
Access to basic water, sanitation and hygiene, waste management and environment cleaning (WASH) in healthcare facilities (HCFs) is critical for infection prevention and control. The WHO/UNICEF 2019 global baseline report on WASH in HCFs indicates that 51 and 23% of those in sub-Saharan Africa have basic access to water and sanitation, respectively. Guided by the political ecology of health theory, this research engaged with 13 key informants, 16 healthcare workers and 31 community members on their experiences on the implementation, use and management of WASH in HCFs. Interviews were conducted in one informal settlement and three rural dispensaries in Kisumu, Kenya from May to September 2019. Findings indicate improvement in water access, yet water quality and other WASH service components remain a challenge even in newly constructed maternity facilities, thus impacting local health promotion efforts. Institutional challenges such as limited financial resources and ecological factors like climate variability and disease outbreaks compromised WASH infrastructure and HCF resilience. To achieve Sustainable Development Goal 3, good health and well-being, as well as Sustainable Development Goal 6, clean water and sanitation, the prioritisation of WASH in HCFs is required at all levels, from the local to the global.
Sources and routes from terrestrial exogenous pollutants affect phytoplankton biomass in reservoir bays
Authors: Hu R. Y. 
Published: May 2021

Abstract
Reservoir bays, at the terrestrial and water boundary, where water fluidity slows down and self-purification ability turns weak, hence they are especially sensitive to terrestrial exogenous pollutants, even resulting in eutrophication. According to N:P, water nutrient types can be divided into N limited, P limited and N + P limited classes. Phytoplankton biomass is represented by chlorophyll a, which is one of the sensitive indicators of water eutrophication. Comprehensively tracing non-point pollution from terrestrial exogenous pollutants (fertilizer, soil release, anthropogenic discharge) to water nutrients that happen in reservoir bays is of great significance. This paper identified the dominant environmental variables and nutrients' limited types of reservoir bays at storage and discharge periods, and constructed a partial least squares structural equation model (PLS-SEM) to explore the impacts of terrestrial exogenous pollutants. Results showed that in the storage period, water contamination mainly came from residential discharge and soil endogenous release, and the total contribution rate reached 61%. In the discharge period, with the increase of rainfall–runoff erosion, the explanatory ability of land use, topography and landscape pattern to water quality increased, up to 58%. The dominant nutrient limited types of reservoir bays were P limited (35%–47%) and N + P limited (35%–59%) at both stages, N limited situations were less than 20% and generally appeared in the storage period. Whatever the nutrient limited type was, phosphorus always had a higher effect on phytoplankton biomass. In the N limited situation, nitrogen came mainly from soil release (total effect = 0.6) and phosphorus from fertilizer (total effect = 0.22) and soil release (total effect = 0.17). In the P limited situation, all three sources had almost high effects on nitrogen, phosphorus, and phytoplankton biomass. In the N + P limited situation, anthropogenic discharge was the main source of nutrients and the primary threat factor for phytoplankton biomass. The approaches employed in this study could be generalized to other basins and the results were significant for early warning and controlling water eutrophication.
EEMD- and VMD-based hybrid GPR models for river streamflow point and interval predictions
Authors: Ghasempour Roghayeh , Azamathulla Hazi Mohammad , Roushangar Kiyoumars 
Published: May 2021

Abstract
Reliable river streamflow (RSF) forecasting is an important issue due to its impact on planning and operation of the water resources system. In this study, based on Lower Upper Bound Estimation (LUBE), hybrid artificial intelligence methods were used for point and interval prediction of monthly RSF. Two states based on stations' own data and upstream stations' data were considered for RSF modeling of the Housatonic River during the period of 1941–2018. Ensemble Empirical Mode Decomposition (EEMD) and Variational Mode Decomposition (VMD) methods were used for enhancing the streamflow point forecasting accuracy. Interval Prediction (PI) was applied for tolerating increased uncertainty. Results showed that in state 1, the error criterion value for the superior model decreased from 0.155 to 0.082 and 0.09 for the EEMD- and VMD-based models, respectively. Generally, hybrid models increased the modeling accuracy between 20% and 40%. Via the integrated approaches, the upstream stations' data was successfully used for streamflow prediction of stations without data. In this state, the PI coverage probability values for the VMD-based model were approximately 12% higher than the single model. Generally, the VMD-based model led to more desirable results due to having higher PI coverage probability and lower mean PI width values.
Quantifying the Fleet Composition at Full Adoption of Shared Autonomous Electric Vehicles: An Agent-based Approach
Authors: Hogeveen Peter , Steinbuch Maarten , Verbong Geert , Hoekstra Auke 
Published: May 2021

Aims:
Exploring the impact of full adoption of fit-for-demand shared and autonomous electric vehicles on the passenger vehicle fleet of a society.

Background:
Shared Eutonomous Electric Vehicles (SAEVs) are expected to have a disruptive impact on the mobility sector. Reduced cost for mobility and increased accessibility will induce new mobility demand and the vehicles that provide it will be fit-for-demand vehicles. Both these aspects have been qualitatively covered in recent research, but there have not yet been attempts to quantify fleet compositions in scenarios where passenger transport is dominated by fit-for-demand, one-person autonomous vehicles.

Objective:
To quantify the composition of the future vehicle fleet when all passenger vehicles are autonomous, shared and fit-for-demand and where cheap and accessible mobility has significantly increased the mobility demand.

Methods:
An agent-based model is developed to model detailed travel dynamics of a large population. Numerical data is used to mimic actual driving motions in the Netherlands. Next, passenger vehicle trips are changed to trips with fit-for-demand vehicles, and new mobility demand is added in the form of longer tips, more frequent trips, modal shifts from public transport, redistribution of shared vehicles, and new user groups. Two scenarios are defined for the induced mobility demand from SAEVs, one scenario with limited increased mobility demand, and one scenario with more than double the current mobility demand. Three categories of fit-for-demand vehicles are stochastically mapped to all vehicle trips based on each trip's characteristics. The vehicle categories contain two one-person vehicle types and one multi-person vehicle type.

Results:
The simulations show that at full adoption of SAEVs, the maximum daily number of passenger vehicles on the road increases by 60% to 180%. However, the total fleet size could shrink by up to 90% if the increase in mobility demand is limited. An 80% reduction in fleet size is possible at more than doubling the current mobility demand. Additionally, about three-quarters of the SAEVs can be small one-person vehicles.

Conclusion:
Full adoption of fit-for-demand SAEVs is expected to induce new mobility demand. However, the results of this research indicate that there would be 80% to 90% less vehicles required in such a situation, and the vast majority would be one-person vehicles. Such vehicles are less resource-intense and, because of their size and electric drivetrains, are significantly more energy-efficient than the average current-day vehicle. This research indicates the massive potential of SAEVs to lower both the cost and the environmental impact of the mobility sector. Quantification of these environmental benefits and reduced mobility costs are proposed for further research.
Intervención en las problemáticas familiares, barrio la divisa, comuna 13 – Medellín
Authors: Vega Fajardo Jeniffer Ximena , Vargas Amézquita Sonia Lucía 
Published: May 2021

Este estudio presenta la intervencio?n en los aspectos emocionales, socioculturales y cognitivos de las diferentes problema?ticas encontradas en las familias del Barrio la Divisa de la ciudad de Medelli?n bajo el enfoque de investigacio?n cualitativa, con disen?o investigacio?n, accio?n – participativa, dividida en tres fases: diagno?stico, planeacio?n - ejecucio?n, y evaluacio?n.
Se lograron disen?ar estrategias para atender cada una de las necesidades que se percibieron en la comunidad en la fase diagno?stica desde siete microproyectos y se realizo? la ejecucio?n de estos, llevando actividades semanales a 45 nin?os y sus familias quienes participaron libremente del proyecto. Para la tercera fase se espera realizar la evaluacio?n de impacto social de la intervencio?n realizada.
Palabras Clave: Intervencio?n social, familia, infancia.
Abstract
This study presents the intervention in the emotional, sociocultural and cognitive aspects of the different problems found in the families of the neighborhood la Divisa of the city of Medelli?n under the qualitative research approach, with research design, action - participatory, divided into three phases: diagnosis, planning - execution, and evaluation.
We managed to design strategies to address each of the needs that were perceived in the community in the diagnostic phase from seven microprojects and the execution of these was carried out, taking weekly activities to 45 children and their families who freely participated in the project. For the third phase, it is expected to carry out the social impact assessment of the intervention carried out.
Keywords: Social intervention, family, childhood.

Resumo
Este estudo apresenta a intervenc?a?o nos aspectos emocionais, socioculturais e cognitivos das diferentes problema?ticas encontradas nas fami?lias do Bairro La Divisa da cidade de Medelli?n sob o enfoque de investigac?a?o qualitativa, com desenho, investigac?a?o, ac?a?o participativa, dividida em tre?s fases: diagno?stico, planejamento –execuc?a?o e avaliac?a?o. Conseguiu-se desenhar estrate?gias para atender cada uma das necessidades que foram percebidas na comunidade na fase de diagno?stico desde sete microprojetos e sua posterior realizac?a?o, efetuando atividades semanais a 45 crianc?as e suas fami?lias que participaram livremente do projeto. Para a terceira fase, se espera realizar a avaliac?a?o do impacto social da intervenc?a?o realizada.
Palavras chave: Intervenc?a?o Social; Fami?lia; Infa?ncia.
Psicologi?a de la religio?n: reconocimiento teo?rico y panorama investigativo
Authors: Cano García Mariluz , Quintero Núñez Helmer 
Published: May 2021

Resumen
En el argumento del presente arti?culo se ostentan algunas revisiones histo?ricas y descriptivas de la psicologi?a de la religio?n; se refieren y diferencian determinadas nociones como “psicologi?a de la religio?n”, “psicologi?a y religio?n”, y “psicologi?a religiosa”, se advierte de las modificaciones conceptuales de diferentes enfoques y corrientes afirmados en el a?rea, como el de naturaleza fenomenolo?gica; y se resen?an algunos estudios de la actualidad investigativa por continentes.
Palabras Clave: psicologi?a, religio?n, naturaleza fenomenolo?gica.
Abstract
In the argument of the present article there are some historical and descriptive reviews of the psychology of religion; they refer and differentiate certain notions such as "psychology of religion", "psychology and religion", and "religious psychology", warns of the conceptual modifications of different approaches and currents within the subdiscipline that are being affirmed, such as nature phenomenological; and some current research studies by continents are reviewed.
Keywords: psychology,religion,phenomenologicalnature.

Resumo
O argumento do presente artigo se fundamenta em algumas reviso?es histo?ricas e descritivas da psicologia da religia?o; se referem e diferenciam determinadas noc?o?es como “psicologia da religia?o”, “psicologia e religia?o”, e “psicologia religiosa”. Se adverte das modificac?o?es conceituais de diferentes enfoques e correntes afirmados na a?rea, como o de natureza fenomenolo?gica; e se resenham alguns estudos da atualidade por continentes.
PALAVRAS CHAVE: psicologia, religia?o, natureza fenomenolo?gica