bluuebunny/crossref_metadata_2025_split huggingface dataset:
Combination of serum and CSF neurofilament-light and neuroinflammatory biomarkers to evaluate ALS
Authors: Brodovitch Alexandre , Boucraut José , Delmont Emilien , Parlanti Amandine , Grapperon Aude-Marie , Attarian Shahram , Verschueren Annie 
Published: January 2021

AbstractThis monocentric prospective study of patient suffering from Amyotrophic lateral sclerosis (ALS) aims to evaluate the prognosis and diagnostic potential of both Neurofilament-Light (Nf-L) and neuroinflammatory biomarkers in serum and CSF. Candidate markers levels were measured using multiplex method in serum of 60 ALS patients, 94 healthy controls of 43 patients suffering from Inflammatory Peripheral Neuropathies (IPN). A comparative CSF analysis was performed for 20 ALS and 17 IPN patients. Among the altered biomarkers, CSF Nf-L level remains the best marker of ALS severity, while serum levels correlate strongly with disease progression. The combination of Nf-L and ICAM-1 concentrations in the CSF and IFN-γ concentration in the serum differentiate ALS patients from IPN patients with improved sensibility and specificity relative to individual biomarkers. A cutoff value of 0.49 for the fitted values of these 3 biomarkers discriminate ALS from IPN patients with a specificity of 100% (78.20–100%) and a sensibility of 85.71% (57.19–98.22%) with an AUC of 0.99 ± 0.01. The measure of Nf-L and neuroinflammatory biomarkers in CSF and serum can be useful biomarkers panel in the differential diagnosis of ALS.
A selectable, plasmid-based system to generate CRISPR/Cas9 gene edited and knock-in mosquito cell lines
Authors: Rozen-Gagnon Kathryn , Yi Soon , Jacobson Eliana , Novack Sasha , Rice Charles M. 
Published: January 2021

AbstractAedes (Ae.) aegypti and Ae. albopictus mosquitoes transmit arthropod-borne diseases around the globe, causing ~ 700,000 deaths each year. Genetic mutants are valuable tools to interrogate both fundamental vector biology and mosquito host factors important for viral infection. However, very few genetic mutants have been described in mosquitoes in comparison to model organisms. The relative ease of applying CRISPR/Cas9-based gene editing has transformed genome engineering and has rapidly increased the number of available gene mutants in mosquitoes. Yet, in vivo studies may not be practical for screening large sets of mutants or possible for laboratories that lack insectaries. Thus, it would be useful to adapt CRISPR/Cas9 systems to common mosquito cell lines. In this study, we generated and characterized a mosquito optimized, plasmid-based CRISPR/Cas9 system for use in U4.4 (Ae. albopictus) and Aag2 (Ae. aegypti) cell lines. We demonstrated highly efficient editing of the AGO1 locus and isolated U4.4 and Aag2 cell lines with reduced AGO1 expression. Further, we used homology-directed repair to establish knock-in Aag2 cell lines with a 3xFLAG-tag at the N-terminus of endogenous AGO1. These experimentally verified plasmids are versatile, cost-effective, and efficiently edit immune competent mosquito cell lines that are widely used in arbovirus studies.
Nestling odour modulates behavioural response in male, but not in female zebra finches
Authors: Golüke Sarah , Bischof Hans-Joachim , Caspers Barbara A. 
Published: January 2021

AbstractStudies investigating parent offspring recognition in birds led to the conclusion that offspring recognition is absent at the early nestling stage. Especially male songbirds were often assumed to be unable to discriminate between own and foreign offspring. However, olfactory offspring recognition in birds has not been taken into account as yet, probably because particularly songbirds have for a long time been assumed anosmic. This study aimed to test whether offspring might be recognised via smell. We presented zebra finch (Taeniopygia guttata) parents either the odour of their own or that of foreign nestlings and investigated whether the odour presentation resulted in a change in the number of head saccades, i.e. the rapid horizontal turning of the head, with which birds scan their environment and which can be used as a proxy of arousal. Our experiment indicates that male zebra finches, in contrast to females, differentiate between their own and foreign offspring based on odour cues, as indicated by a significant differences in the change of head saccadic movements between males receiving the own chick odour and males receiving the odour of a foreign chick. Thus, it provides behavioural evidence for olfactory offspring recognition in male zebra finches and also the existence of appropriate phenotypic odour cues of the offspring. The question why females do not show any sign of behavioural response remains open, but it might be likely that females use other signatures for offspring recognition.
Macromolecule suppressed GABA levels show no relationship with age in a pediatric sample
Authors: Bell Tiffany , Stokoe Mehak , Harris Ashley D. 
Published: January 2021

AbstractThe inhibitory neurotransmitter γ-Aminobutyric acid (GABA) plays a crucial role in cortical development. Therefore, characterizing changes in GABA levels during development has important implications for the study of healthy development and developmental disorders. Brain GABA levels can be measured non-invasively using GABA-edited magnetic resonance spectroscopy (MRS). However, the most commonly used editing technique to measure GABA results in contamination of the GABA signal with macromolecules (MM). Therefore, GABA measured using this technique is often referred to as GABA+ . While few in number, previous studies have shown GABA+ levels increase with age during development. However, these studies are unable to specify whether it is specifically GABA that is increasing or, instead, if levels of MM increase. In this study, we use a GABA-editing technique specifically designed to suppress the MM signal (MM-supp GABA). We find no relationship between MM-supp GABA and age in healthy children aged 7–14 years. These findings suggest that the relationship between GABA+ and age is driven by changes in MM levels, not by changes in GABA levels. Moreover, these findings highlight the importance of accounting for MM levels in MRS quantification.
Anthropogenic interferences lead to gut microbiome dysbiosis in Asian elephants and may alter adaptation processes to surrounding environments
Authors: Moustafa Mohamed Abdallah Mohamed , Chel Hla Myet , Thu May June , Bawm Saw , Htun Lat Lat , Win Mar Mar , Oo Zaw Min , Ohsawa Natsuo , Lahdenperä Mirkka , Mohamed Wessam Mohamed Ahmed , Ito Kimihito , Nonaka Nariaki , Nakao Ryo , Katakura Ken 
Published: January 2021

AbstractHuman activities interfere with wild animals and lead to the loss of many animal populations. Therefore, efforts have been made to understand how wildlife can rebound from anthropogenic disturbances. An essential mechanism to adapt to environmental and social changes is the fluctuations in the host gut microbiome. Here we give a comprehensive description of anthropogenically induced microbiome alterations in Asian elephants (n = 30). We detected gut microbial changes due to overseas translocation, captivity and deworming. We found that microbes belonging to Planococcaceae had the highest contribution in the microbiome alterations after translocation, while Clostridiaceae, Spirochaetaceae and Bacteroidia were the most affected after captivity. However, deworming significantly changed the abundance of Flavobacteriaceae, Sphingobacteriaceae, Xanthomonadaceae, Weeksellaceae and Burkholderiaceae. These findings may provide fundamental ideas to help guide the preservation tactics and probiotic replacement therapies of a dysbiosed gut microbiome in Asian elephants. More generally, these results show the severity of anthropogenic activities at the level of gut microbiome, altering the adaptation processes to new environments and the subsequent capability to maintain normal physiological processes in animals.
Plasma fibrinogen in the diagnosis of periprosthetic joint infection
Authors: Yang Fei , Zhao Chenyu , Huang Rong , Ma Hui , Wang Xiaohe , Wang Guodong , Zhao Xiaowei 
Published: January 2021

AbstractPeriprosthetic joint infections (PJIs) have become the most catastrophic complication for patients after arthroplasty. Although previous studies have found that many biomarkers have good performance for diagnosing PJI, early diagnosis remains challenging and a gold standard is lacking. This study aimed to investigate the diagnostic accuracy of plasma fibrinogen (FIB) in detecting PJI compared to other traditional biomarks (CRP, WBC and ESR). A total of 156 patients (including 57 PJI and 99 non-PJI patients) who underwent revision arthroplasty were retrospectively reviewed from 01/2014 to 01/2020. The diagnostic criteria of PJI were mainly based on the definition from the evidence-based definition for periprosthetic joint infection in 2018. The optimal plasma FIB predictive cutoff was 4.20 g/L, the sensitivity of the plasma fibrinogen was 0.860, the specificity was 0.900, the positive predictive value (PPV) was 0.831, and the negative predictive value (NPV) was 0.908. The area under the curve (AUC) value of plasma fibrinogen was 0.916 (95% CI 0.869–0.964), and the CRP, ESR and WBC levels had AUCs of 0.901, 0.822 and 0.647, respectively. Plasma FIB demonstrated better diagnostic strength compared with that of other serum biomarkers before revision arthroplasty. It represents a new horizon for the diagnosis of PJI due to the diagnosis values and cost-effective features.
Tropical rhodolith beds are a major and belittled reef fish habitat
Authors: Moura Rodrigo L. , Abieri Maria L. , Castro Guilherme M. , Carlos-Júnior Lélis A. , Chiroque-Solano Pamela M. , Fernandes Nicole C. , Teixeira Carolina D. , Ribeiro Felipe V. , Salomon Paulo S. , Freitas Matheus O. , Gonçalves Juliana T. , Neves Leonardo M. , Hackradt Carlos W. , Felix-Hackradt Fabiana , Rolim Fernanda A. , Motta Fábio S. , Gadig Otto B. F. , Pereira-Filho Guilherme H. , Bastos Alex C. 
Published: January 2021

AbstractUnderstanding habitat-level variation in community structure provides an informed basis for natural resources’ management. Reef fishes are a major component of tropical marine biodiversity, but their abundance and distribution are poorly assessed beyond conventional SCUBA diving depths. Based on a baited-video survey of fish assemblages in Southwestern Atlantic’s most biodiverse region we show that species composition responded mainly to the two major hard-bottom megahabitats (reefs and rhodolith beds) and to the amount of light reaching the bottom. Both megahabitats encompassed typical reef fish assemblages but, unexpectedly, richness in rhodolith beds and reefs was equivalent. The dissimilar fish biomass and trophic structure in reefs and rhodolith beds indicates that these systems function based on contrasting energy pathways, such as the much lower herbivory recorded in the latter. Rhodolith beds, the dominant benthic megahabitat in the tropical Southwestern Atlantic shelf, play an underrated role as fish habitats, and it is critical that they are considered in conservation planning.
The amphibian peptide Yodha is virucidal for Zika and dengue viruses
Authors: Lee Song Hee , Kim Eui Ho , O’neal Justin T. , Dale Gordon , Holthausen David J. , Bowen James R. , Quicke Kendra M. , Skountzou Ioanna , Gopal Shyla , George Sanil , Wrammert Jens , Suthar Mehul S. , Jacob Joshy 
Published: January 2021

AbstractZika virus (ZIKV) has emerged as a serious health threat in the Americas and the Caribbean. ZIKV is transmitted by the bite of an infected mosquito, sexual contact, and blood transfusion. ZIKV can also be transmitted to the developing fetus in utero, in some cases resulting in spontaneous abortion, fetal brain abnormalities, and microcephaly. In adults, ZIKV infection has been correlated with Guillain–Barre syndrome. Despite the public health threat posed by ZIKV, neither a vaccine nor antiviral drugs for use in humans are currently available. We have identified an amphibian host defense peptide, Yodha, which has potent virucidal activity against ZIKV. It acts directly on the virus and destroys Zika virus particles within 5 min of exposure. The Yodha peptide was effective against the Asian, African, and South American Zika virus strains and has the potential to be developed as an antiviral therapeutic in the fight against Zika virus. The peptide was also effective against all four dengue virus serotypes. Thus, Yodha peptide could potentially be developed as a pan-therapeutic for Zika and dengue viruses.
Magnetic resonance microscopy of samples with translational symmetry with FOVs smaller than sample size
Authors: Serša Igor 
Published: January 2021

AbstractIn MRI, usually the Field of View (FOV) has to cover the entire object. If this condition is not fulfilled, an infolding image artifact is observed, which suppresses visualization. In this study it is shown that for samples with translational symmetry, i.e., those consisting of identical objects in periodic unit cells, the FOV can be reduced to match the unit cell which enables imaging of an average object, of which the signal is originated from all unit cells of the sample, with no punishment by a loss in signal-to-noise ratio (SNR). This theoretical prediction was confirmed by experiments on a test sample with a 7 × 7 mm2unit cell arranged in a 3 × 3 matrix which was scanned by the spin-echo and by single point imaging methods. Effects of experimental imperfections in size and orientation mismatch between FOV and unit cell were studied as well. Finally, this method was demonstrated on a 3D periodic sample of tablets, which yielded well-resolved images of moisture distribution in an average tablet, while single tablet imaging provided no results. The method can be applied for SNR increase in imaging of any objects with inherently low signals provided they can be arranged in a periodic structure.
Map and sequence-based chromosome walking towards cloning of the male fertility restoration gene Rf5 linked to R11 in sunflower
Authors: Ma Guojia , Long Yunming , Song Qijian , Talukder Zahirul I. , Shamimuzzaman Md , Qi Lili 
Published: January 2021

AbstractThe nuclear fertility restorer gene Rf5 in HA-R9, originating from the wild sunflower species Helianthus annuus, is able to restore the widely used PET1 cytoplasmic male sterility in sunflowers. Previous mapping placed Rf5 at an interval of 5.8 cM on sunflower chromosome 13, distal to a rust resistance gene R11 at a 1.6 cM genetic distance in an SSR map. In the present study, publicly available SNP markers were further mapped around Rf5 and R11 using 192 F2 individuals, reducing the Rf5 interval from 5.8 to 0.8 cM. Additional SNP markers were developed in the target region of the two genes from the whole-genome resequencing of HA-R9, a donor line carrying Rf5 and R11. Fine mapping using 3517 F3 individuals placed Rf5 at a 0.00071 cM interval and the gene co-segregated with SNP marker S13_216392091. Similarly, fine mapping performed using 8795 F3 individuals mapped R11 at an interval of 0.00210 cM, co-segregating with two SNP markers, S13_225290789 and C13_181790141. Sequence analysis identified Rf5 as a pentatricopeptide repeat-encoding gene. The high-density map and diagnostic SNP markers developed in this study will accelerate the use of Rf5 and R11 in sunflower breeding.
Consecutive sexual maturation observed in a rock shell population in the vicinity of the Fukushima Daiichi Nuclear Power Plant, Japan
Authors: Horiguchi Toshihiro , Kawamura Kayoko , Ohta Yasuhiko 
Published: January 2021

AbstractIn 2012, after the accident at the Fukushima Daiichi Nuclear Power Plant (FDNPP) that followed the Tohoku earthquake and tsunami in March 2011, no rock shell (Thais clavigera; currently recognized as Reishia clavigera; Gastropoda, Neogastropoda, Muricidae) specimens were found near the plant from Hirono to Futaba Beach (a distance of approximately 30 km). In July 2016, however, rock shells were again found to inhabit the area. From April 2017 to May 2019, we collected rock shell specimens monthly at two sites near the FDNPP (Okuma and Tomioka) and at a reference site ~ 120 km south of the FDNPP (Hiraiso). We examined the gonads of the specimens histologically to evaluate their reproductive cycle and sexual maturation. The gonads of the rock shells collected at Okuma, ~ 1 km south of the FDNPP, exhibited consecutive sexual maturation during the 2 years from April 2017 to May 2019, whereas sexual maturation of the gonads of specimens collected at Hiraiso was observed only in summer. The consecutive sexual maturation of the gonads of the specimens collected at Okuma might not represent a temporary phenomenon but rather a site-specific phenotype, possibly caused by specific environmental factors near the FDNPP.
Crystallographic texture dependent bulk anisotropic elastic response of additively manufactured Ti6Al4V
Authors: Pantawane Mangesh V. , Yang Teng , Jin Yuqi , Joshi Sameehan S. , Dasari Sriswaroop , Sharma Abhishek , Krokhin Arkadii , Srinivasan Srivilliputhur G. , Banerjee Rajarshi , Neogi Arup , Dahotre Narendra B. 
Published: January 2021

AbstractRapid thermokinetics associated with laser-based additive manufacturing produces strong bulk crystallographic texture in the printed component. The present study identifies such a bulk texture effect on elastic anisotropy in laser powder bed fused Ti6Al4V by employing an effective bulk modulus elastography technique coupled with ultrasound shear wave velocity measurement at a frequency of 20 MHz inside the material. The combined technique identified significant attenuation of shear velocity from 3322 ± 20.12 to 3240 ± 21.01 m/s at 45$$^\circ$$

∘

and 90$$^\circ$$

∘

orientations of shear wave plane with respect to the build plane of printed block of Ti6Al4V. Correspondingly, the reduction in shear modulus from 48.46 ± 0.82 to 46.40 ± 0.88 GPa was obtained at these orientations. Such attenuation is rationalized based on the orientations of $$\alpha ^\prime$$

α
′

crystallographic variants within prior columnar $$\beta$$
β
grains in additively manufactured Ti6Al4V.
Body roll amplitude and timing in backstroke swimming and their differences from front crawl at the same swimming intensities
Authors: Gonjo Tomohiro , Fernandes Ricardo J. , Vilas-Boas João Paulo , Sanders Ross 
Published: January 2021

AbstractThe current study investigated body roll amplitude and timing of its peak in backstroke and compared them with front crawl swimming. Nineteen anatomical landmarks were digitised using 80 swimming trial videos (ten swimmers × two techniques × four intensities) recorded by two above- and four below-water cameras. One upper-limb cycle was analysed for each trial, and shoulder and hip roll, whole-body roll (WBR), and WBR due to the buoyant torque (WBRBT) were obtained. Main effects of intensity and technique on the amplitude and timing to reach the peak in those variables were assessed by two-way repeated-measures ANOVA. Swimmers decreased their WBRBT amplitude with an increase in the intensity in both techniques (p ≤ 0.005). The same result was observed for the amplitude of WBR, shoulder roll, and hip roll only in front crawl (p ≤ 0.017). Swimmers maintained the timing of peak WBRBT in both techniques, while they shifted the timing of WBR and hip roll peak toward the beginning of the cycle when increasing the intensity in front crawl (p ≤ 0.017). In conclusion, swimmers maintain the amplitude of WBR, shoulder roll, and hip roll in backstroke when the intensity increases, whereas they reduce the amplitude of all rolls in front crawl.
Assessing seal carcasses potentially subjected to grey seal predation
Authors: van Neer Abbo , Gross Stephanie , Kesselring Tina , Grilo Miguel L. , Ludes-Wehrmeister Eva , Roncon Giulia , Siebert Ursula 
Published: January 2021

AbstractIn order to conduct an objective evaluation of potential ecological effects of grey seal predation on marine mammals, it is essential to establish a broad knowledge base helping in the thorough identification of such cases during post-mortem examination. The aim of this work is to report and discuss outcomes resulting from a retrospective evaluation of harbour (Phoca vitulina) and grey seal (Halichoerus grypus) stranding and necropsy data (n = 3274). In addition, the results are compared to a recent case of definite grey seal predation from Germany as well as reports from other countries. Carcasses potentially subjected to grey seal predation show severe lacerations with a circular pattern leaving a smooth, linear and cut-like wound margin. Large parts of skin and underlying tissue are detached from the body and loss of blubber is common. Occurrence frequencies of encountered lesions are presented and a list of parameters to be used for the assessment of similar cases as well as a complementary decision tree are suggested. With the proposed parameters, categories and tools, a baseline can be built in order to facilitate the standardised recognition of predation cases during post-mortem examinations of seals between groups working with populations across several geographic ranges.
Schizotypy in Parkinson’s disease predicts dopamine-associated psychosis
Authors: Oehrn Carina R. , Schönenkorb Jana , Timmermann Lars , Nenadić Igor , Weber Immo , Grant Phillip 
Published: January 2021

AbstractPsychosis is the most common neuropsychiatric side-effect of dopaminergic therapy in Parkinson’s disease (PD). It is still unknown which factors determine individual proneness to psychotic symptoms. Schizotypy is a multifaceted personality trait related to psychosis-proneness and dopaminergic neurotransmission in healthy subjects. We investigated whether (1) PD patients exhibit lower schizotypy than controls and (2) dopamine-related neuropsychiatric side-effects can be predicted by higher schizotypy. In this cross-sectional study, we used the Oxford-Liverpool Inventory of Feelings and Experiences in 56 PD patients (12 women, mean ± sd age: 61 ± 11 years) receiving their usual dopaminergic medication and 32 age-matched healthy controls (n = 32; 18 women, mean ± sd age: 57 ± 6 years). We further compared schizotypy scores of patients with (n = 18, 32.1%) and without previously experienced psychosis. We found that patients exhibited lower schizotypy than controls. Further, patients with a history of psychosis exhibited higher schizotypy than patients without these symptoms. Using an information theoretic measure and a machine learning approach, we show that schizotypy yields the greatest predictive value for dopamine-associated hallucinations compared to other patient characteristics and disease related factors. Our results indicate an overlap between neural networks associated with schizotypy and the pathophysiology of PD and a relationship between schizotypy and psychotic side-effects of dopaminergic medication.