bluuebunny/crossref_metadata_2025_split huggingface dataset:
Erythrocyte Membrane-Enveloped Molybdenum Disulfide Nanodots for Biofilm Elimination on Implants Via Toxin Neutralization and Immune Modulation
Authors: Shi Tingwang , Ruan Zesong , Wu Jianrong , Zou Weijuan , Cai Xiaojun , Zheng Yuanyi , Chen Yunfeng 
Published: December 2021

Abstract
Backgrounds:
Implant-related infections (IRIs) caused by bacterial biofilms remain a prevalent but tricky clinical issue, which are characterized by drug resistance, toxin impairment and immunity suppression. Recently, antimicrobial therapies based on reactive oxygen species (ROS) or hyperthermia have been developed to effectively destroy biofilms. However, all of them have failed to simultaneously focus on the immunosuppressive microenvironment of biofilms and tissue damage caused by bacterial toxins.
Results
Herein, we proposed a one-arrow-three-hawks strategy to orchestrate hyperthermia/ROS antibiofilm therapy, toxin neutralization and immunomodulatory therapies through engineering a bioinspired erythrocyte membrane-enveloped molybdenum disulfide nanodots (EM@MoS2) nanoplatform. In biofilm microenvironment, pore-forming toxins actively attack the erythrocyte membranes on the nanodots and be detained, thus keeping toxins away from their targets and mitigating tissue damage. Under near-infrared laser irradiation, MoS2 nanodots, with superb photothermal and peroxidase-like properties, exert a powerful synergistic antibiofilm effect. More intriguingly, we initially identified that they possess the ability to reverse the immunosuppressive microenvironment through skewing the macrophages from an anti-inflammatory phenotype to a proinflammatory phenotype, which would promote the elimination of biofilm debris and prevention of infection relapse. Systematic in vitro and in vivo evaluations have demonstrated that EM@MoS2 achieves remarkable antibiofilm effect.
Conclusion
The current study integrated the functions of hyperthermia/ROS therapy, virulence clearance and immune regulation, which could provide an effective paradigm for IRIs therapy.
Alkali Can Accelerate The Cellulose Dissolution In Aqueous 1-Ethyl-3-Methylimidazole Acetate (EmimAc/10% Water)
Authors: Ni Liufang , Lu Xingmei , Yu Jing , Lin Changmei , Cao Xiaoxia , Cao Shilin , Ma Xiaojuan , Gao Haili 
Published: December 2021

Abstract
Ionic liquids are potential and successful cellulose solvent but still suffer technical and economic issues in the cellulose commercialization. In this work, a relative low-viscosity aqueous 1-ethyl-3-methylimidazole acetate (EmimAc with 10% water) was used instead of EmimAc to dissolve cellulose; the results showed that adding NaOH to water can significantly accelerate cellulose dissolution and the cellulose solubility increased with the NaOH concentration in the EmimAc/10% water solution. NaOH can weaken the strong interaction between water and EmimAc because it can bond preferentially with water by hydrogen bonding and therefore release Ac - from Ac - -water cluster; which can enhance the reaction between Emim + and Ac - and therefore improve the cellulose dissolution. Unfortunately, the NaOH introduction inevitably cause a cellulose degradation via peeling reaction.
High-Throughput Growth of HfO2 Films Using Temperature-gradient Laser Chemical Vapor Deposition
Authors: TU Rong , LIU Ziming , WANG Chongjie , LU Pengjian , GUO Bingjian , XU Qingfang , LI Bao-Wen , WANG Chuanbin , ZHANG Lianmeng , Zhang Song 
Published: December 2021

Abstract
The use of hafnia (HfO2) has facilitated recent advances in combining uprated dielectric layers (UDLs) and environmental barriers (EBs) in supercomputers. However, an extremely low deposition rate limits further development and fabrication efficiency of HfO2 films. In this study, high-throughput growth of HfO2 films was realized via laser chemical vapor deposition using a laser spot with a gradient temperature distribution. In HfO2 films fabricated by a single growth process, four regions with different morphologies could be discerned for deposition temperatures increasing from 1300 K to 1600 K: leaf-like, pyramid-like, bromeliad-like and pinecone-like. Two growth modes were observed for Regions I and II: Stranski-Krastanov and Volmer-Weber. Regions III and IV contained coexisting monoclinic and tetragonal HfO2 grains with an in-plane boundary for m-HfO2 (-110) {111}//t-HfO2 (1-11) {111}. The maximum deposition rate reached 362 μm/h, which was 102 - 104 times higher than that obtained using existing deposition methods.
Reading and Writing The Combined Effects of Regular Classroom and Remedial Learning Programs on the Reading Achievement of First Grade Pupils
Authors: Steve Bissonnette , Boyer Christian , Morneau-Guérin Frédéric 
Published: December 2021

Abstract
In 2003, two school-based programs for teaching reading in English were introduced in Québec: the Accelerated Development of Reading (ADOR) program for first graders and, for ADOR pupils with weak reading skills, the Intensive Intervention in Reading (IIR) support program. Explicit and direct instruction provides the framework for ADOR and IIR. Two quasi-experimental studies were conducted to measure the effects of the ADOR and IIR programs compared to regular reading instruction and remediation. In both studies, the combined effects of ADOR and IIR were measured on two separate cohorts of grade 1 pupils over a full school year. This study shows that the ADOR and IIR programs result in superior reading performance and the largest effects were on subtests measuring reading comprehension.
Histological and Top-Down Proteomic Analyses of the Visual Pathway Using the Cuprizone Demyelination Model
Authors: Almuslehi Mohammed S. M. , Sen Monokesh K. , Shortland Peter J. , Mahns David A. , Coorssen Jens R. 
Published: December 2021

Abstract
A change in visual perception is a frequent early symptom of multiple sclerosis (MS), the pathoetiology of which remains unclear. Following a slow demyelination process caused by 12 weeks of low-dose (0.1%) cuprizone (CPZ) consumption, histology and proteomics were used to investigate components of the visual pathway in young adult mice. Histological investigation did not identify demyelination or gliosis in the optic tracts, pretectal nuclei, superior colliculi, lateral geniculate nuclei or visual cortices. However, top-down proteomic assessment of the optic nerve/tract revealed a significant change in the abundance of 34 spots in high-resolution 2D gels. Subsequent liquid chromatography-tandem mass spectrometry analysis identified alterations in 75 proteoforms. Literature mining revealed the relevance of these proteoforms in terms of proteins previously implicated in animal models and human MS. Importantly, 24 proteoforms were not previously described in any animal models of MS or MS itself. Bioinformatic analysis indicated involvement of these proteoforms in cytoskeleton organization, metabolic dysregulation, protein aggregation, and axonal support. Collectively, these results indicate that continuous CPZ-feeding, which evokes a slow demyelination, results in proteomic changes that precede any clear histological changes in the visual pathway and that these proteoforms may be potential early markers of degenerative demyelinating conditions.
Dynamics Study of Constant DI and Constant TR Control for Cardiac Alternans Based on a Two Dimensional Cellular Automata Model
Authors: Xiong Min , Sun Kai , Su Xiaowen , Tolkacheva Elena G. , Zhao Xiaopeng 
Published: December 2021

Abstract
As a precursor for cardiac arrhythmias such as atrial and ventricular fibrillations, which could cause sudden cardiac death (SCD), cardiac alternans is essentially an unstable heart rhythm with alternating long and short action potential durations (APD) of cardiac myocytes that usually occurs under fast pacing conditions. In this paper, the constant TR control method based on global pseudo-electrocardiogram (ECG) is studied and compared with the local constant diastolic interval (DI) control method using a 2-dimensional (2-D) cellular automata model (CAM), aiming at preventing or eliminating cardiac alternans before arrhythmias. The results show that both the constant TR and constant DI control methods are effective in stabling the alternans to a smaller basic cycle length (BCL). Also, the efficacy of the two control approaches depends on the “decrease step” Δ in the downsweep protocol, and a smaller Δ could significantly improve their performance. Besides, in general, constant TR control is superior to constant DI control in alternans prevention when a relatively large Δ is adopted.
The Threshold of Visceral Adipose Tissue Areas Measured With QCT On Cardiometabolic Risk Factors In A Nationwide Study In China
Authors: Zha Xiaojuan , Lu Yanhui , Li Yongli , Yan Dong , Ran Limei , Lu Yong , Yang Yingying , Liu Yandong , Zhao Kaiping , Glen Blake M. , Zou Mingzhu , Cheng Xiaoguang , Chen Min 
Published: December 2021

Abstract
Background: Abdominal adiposity is associated with increased cardiometabolic risk and visceral adipose tissue (VAT) area obtained from computed tomography (CT) is a key anthropometric index. The present study investigated the VAT criteria on cardiometabolic risk factors in a large-scale nationwide Chinese population.Method: A total of 21,772 adults who underwent a low dose chest CT (LDCT) for lung cancer screening at one of 13 health checkup centers throughout China and had complete ancillary health records were evaluated. Abdominal VAT area at the center of L2 vertebra was measured with Mindways quantitative CT software using the existing LDCT dataset. Relevant anthropometric, serum and blood pressure measurements were obtained from the participants’ records. The VAT distribution of the LDCT cohort was age-adjusted using the Chinese national census. Results: The prevalence of metabolic syndrome based on the Chinese national guidelines was 29.5% and 10.5% in men and women respectively. Using ROC curves, the optimum threshold values for VAT area to identify metabolic syndrome were 213 cm2 in men, and 136 cm2 in women. For participants with VAT area above threshold, the odds ratio (95% confidence interval) for metabolic syndrome was 6.15 (5.65, 6.69) and 9.25 (7.58, 11.4) for men and women respectively. A further analysis of population attributable risk showed that VAT above threshold was significantly associated with an increased risk for metabolic syndrome.Conclusion: In this large-scale nationwide population of China, sex-specific threshold values for VAT area measured by CT were established to identify the risk of metabolic syndrome. VAT area is a key anthropometric index of interventions to control cardiometabolic risk.
Neutralising reactivity against SARS-CoV-2 B.1.617.2 (Delta) variant by vaccination status and pre-exposure
Authors: Lavezzo Enrico , Pacenti Monia , Manuto Laura , Boldrin Caterina , Cattai Margherita , Grazioli Marco , Bianca Federico , Sartori Margherita , Caldart Federico , Castelli Gioele , Nicoletti Michele , Nieddu Eleonora , Salvadoretti Elisa , Labella Beatrice , Fava Ludovico , Vanuzzo Maria , Lisi Vittoria , Antonello Maria , Grimaldi Carmela , Zulian Chiara , Vecchio Claudia Del , Plebani Mario , Padoan Andrea , Cirillo Daniela Maria , Brazzale Alessandra , Tonon Giovanni , Toppo Stefano , Dorigatti laria , Crisanti Andrea 
Published: December 2021

Abstract
In February and March 2020, one of the first Italian clusters of SARS-CoV-2 infection was detected in the municipality of Vo’. Positive subjects were followed up at 2 and 9 months post-infection with different immuno-assays and a micro-neutralisation test. Here we report on the results of the third serosurvey conducted in the same population in June 2021, 15 months post-infection, when we tested 61% of the infected individuals (n=76). Antibodies against the spike (S) antigen significantly decreased (P<0.006, Kruskal-Wallis test) among unvaccinated subjects (n=35) and increased (P<0.0001) in vaccinated individuals (n=41), whereas those against the nucleocapsid (N) decreased in the whole cohort. From the comparison with two control groups (naïve Vo’ inhabitants (n=20) and healthcare workers (HCW, n=61)), subjects vaccinated post exposure (hybrid immunity) had higher antibody levels (P<0.0001) than subjects vaccinated when naïve. Two doses of vaccine elicited stronger anti-S antibody response than natural infection (P<0.0001). Finally, the neutralising reactivity of sera against the B.1.617.2 (Delta) was lower than compared to the B.1 strain (median 1:320 versus 1:1280 1/dil, P<0.0001, and 1:640 versus 1:2560 1/dil, P=0.0014, after one or two vaccine doses, respectively), although subjects with hybrid immunity maintained neutralising titres above 1:40 1/dil.
Interpreting Mass Spectra Differing from Their Peptide Models by Several Modifications
Authors: Lysiak Albane , Fertin Guillaume , Jean Géraldine , Tessier Dominique 
Published: December 2021

Abstract
Background: In proteomics, mass spectra representing peptides carrying multiple unknown modifications are particularly difficult to interpret. This issue results in a large number of unidentified spectra.Methods: We developed SpecGlob, a dynamic programming algorithm that aligns pairs of spectra – each pair given by a Peptide-Spectrum Match (PSM) – provided by any Open Modification Search (OMS) method. For each PSM, SpecGlob computes the best alignment according to a given score system, interpreting the mass delta within the PSM as one or several unspecified modification(s). All the alignments are provided in a file, using a specific syntax. These alignments are then post-processed by an additional algorithm, which aims at interpreting the detected modifications.Results: Using a large collection of theoretical spectra generated from the human proteome, we demonstrate that running SpecGlob as a post-analysis of an OMS method can significantly increase the number of correctly interpreted spectra, since SpecGlob is able to infer several, and possibly many, modifications. The post-processing algorithm is able to interpret unambiguously most of the modifications detected by SpecGlob in PSMs. In addition, we performed an extensive analysis to provide insight into the potential reasons for incomplete or erroneous interpretations that may remain after alignments of PSMs.Conclusion: SpecGlob is able to correctly align spectra that differ by one or more modification(s) without any a priori. Since SpecGlob explores all possible alignments that may explain the mass delta within a PSM, it reduces interpretation errors generated by incorrect assumptions about the modifications present in the sample or the number and the specificity of modifications carried by peptides. Our results demonstrate that SpecGlob should be relevant to align experimental spectra, even if this consists in a more challenging task.
Controlling Volatility of Wind-Solar Power In Germany
Authors: Lustfeld Hans 
Published: December 2021

Abstract
The main advantage of wind-solar power is the electric power production free of CO2. Its main disadvantage is the huge volatility of the system [national electric energy consumption powered by wind-solar power]. In fact, if this power production, averaged over one year, corresponds to the averaged electric consumption and is intended to replace all other electric power generating devices, then controlling the volatility of this system by using storage alone requires huge capacities of about 30TWh, capacities not available in Germany. However, based on German power data over the last six years (2015 till 2020) we show that the required storage capacity is decisively reduced, provided i) a surplus of wind-solar power is supplied, ii) smart meters are installed, iii) a different kind of wind turbines and solar panels is partially used, iv) a novel function describing this volatile system, is introduced. The new function, in turn, depends on three characteristic numbers, which means, that the volatility of this system is characterized by those numbers. When applying our schemes the results suggest that all the present electric energy in Germany can be obtained from controlled wind-solar power. And our results indicate that controlled wind-solar power can produce the energy for transportation, warm water, space heating and in part for process heating, requirering an increase of the electric energy production by a factor of 5. Then, however, a huge number of wind turbines and solar panels is required changing the appearance of German landscapes fundamentally.
Melanocytic Nevi In Sentinel Lymph Nodes - Association With Cutaneous Nevi And Clinical Relevance In Patients With Cutaneous Melanomas
Authors: Kretschmer Lutz , Schnabel Viktor , Kromer Christian , Bauer-Büntzel Christoph , Richter Annika , Bremmer Felix , Kück Fabian , Julius Katharina , Mitteldorf Chirsitna , Schön Michael P. 
Published: December 2021

Abstract
Purpose: Melanocytic nevi in lymph nodes (NNs) are an important histological differential diagnosis of initial sentinel lymph node (SN) metastasis in melanoma. Our aim was to associate NN in SNs with clinicopathologic features and survival rates in 1 250 patients with SN biopsy for melanoma. Methods: To compare patients with present and absent NN, we used Fisher's exact test, Mann-Whitney U test, and multivariate logistic regression models in this retrospective observational study based on a prospectively maintained institutional database.Results: NN prevalence in axillary, cervical, and groin SNs was 16.5%, 19.4%, and 9.8%, respectively. NN were observed in combination with all growth patterns of melanoma, but more frequently when the primary was histologically associated with a cutaneous nevus. We observed a decreasing NN prevalence with increasing SN metastasis diameter. Multiple logistic regression determined a significantly increased NN probability for SNs of the neck or axilla, for individuals with ≥50 cutaneous nevi, midline primary melanomas, and for individuals who reported non-cutaneous malignancies in their parents. Cancer in parents was also significantly more frequently reported by melanoma patients who had more than 50 cutaneous nevi. In SN-negative patients, NN indicated a tendency for slightly lower melanoma-specific survival.Conclusions: We found a highly significant association between NN diagnosis and multiple cutaneous nevi and provided circumstantial evidence that cutaneous nevi in the drainage area of lymph nodes are particularly important. The trend toward lower melanoma-specific survival in SN-negative patients with NN suggests that careful differentiation of SN metastases is important.
1D and 2D NMR spectra of coffee from 27 countries
Authors: Osorio Javier , Arana Victoria A. , Medina Jessica M. , Alarcon Rodrigo , Moreno Edgar , Wist Julien 
Published: December 2021

Abstract
715 green coffee samples were gathered by Almacafé S.A. (Bogotá, Colombia) between 2012 and 2014 from 27 different countries and analysed at the Nuclear Magnetic Resonance (NMR) laboratory at Universidad del Valle (Cali, Colombia). Over 1000 methanolic coffee extracts were prepared and a total of 4563 spectra were acquired in a fully automatic manner using a 400 MHz NMR spectrometer (Bruker Biospin, Germany). The dataset spans the variance that could be expected for an industrial application of origin monitoring, including samples from different harvest time, collected over several years and processed by at least two distinct operators. The resulting 1D and 2D spectra can be used to develop and evaluate feature extraction methods, multivariate algorithms and automation monitoring techniques, as a dataset for teaching, or as a reference for new studies on similar samples and approach.
Clinical Implications of Oral Assessment In Elderly Patients With Acute Heart Failure: A Single-Center, Retrospective Study
Authors: Uemura Yusuke , Shibata Rei , Ishikawa Haruna , Nagahori Ayumi , Katsumi Yuta , Takemoto Kenji , Ishikawa Shinji , Murohara Toyoaki , Watarai Masato 
Published: December 2021

Abstract
Background: Oral health problems are common and are associated with various geriatric conditions in older adults. The importance of oral health has not been fully highlighted in the assessment and management of patients with heart failure. Here, we investigated the clinical implications of oral assessment in elderly patients with acute heart failure.Methods: We evaluated oral health using the revised oral assessment guide in 77 patients aged 65 years or older who were admitted to hospital for acute heart failure. Poor oral health was defined as a revised oral assessment guide score ≥ 9.Results: Poor oral health was identified in 66.2% of the patients. Patients with poor oral health had high prevalence of decreased physical function, undernutrition, and cognitive impairment. A reduction in the Barthel Index, as an indicator of activities of daily living during hospitalization, was significant in the enrolled patients. The Barthel Index decreased more in patients with poor oral health than those with normal oral health. Furthermore, the revised oral assessment guide score on admission was found to be the only independent predictor of changes in the Barthel Index during hospitalization in the multivariate regression analyses. Conclusions: Oral assessment using the revised oral assessment guide during hospitalization could provide useful information for the management of elderly heart failure patients.Trail registration: Patients were retrospectively registered.
CCST: Cell clustering for spatial transcriptomics data with graph
neural network
Authors: Li Jiachen , Chen Siheng , Pan Xiaoyong , Yuan Ye , Shen Hong-bin 
Published: December 2021

Abstract
Spatial transcriptomics data can provide high-throughput gene expression profiling and spatial structure of tissues simultaneously. An essential question of its initial analysis is cell clustering. However, most existing studies rely on only gene expression information and cannot utilize spatial information efficiently. Taking advantages of two recent technical development, spatial transcriptomics and graph neural network, we thus introduce CCST, Cell Clustering for Spatial Transcriptomics data with graph neural network, an unsupervised cell clustering method based on graph convolutional network to improve ab initio cell clustering and discovering of novel sub cell types based on curated cell category annotation. CCST is a general framework for dealing with various kinds of spatially resolved transcriptomics. With application to five in vitro and in vivo spatial datasets, we show that CCST outperforms other spatial cluster approaches on spatial transcriptomics datasets, and can clearly identify all four cell cycle phases from MERFISH data of cultured cells, and find novel functional sub cell types with different micro-environments from seqFISH+ data of brain, which are all validated experimentally, inspiring novel biological hypotheses about the underlying interactions among cell state, cell type and micro-environment.