bluuebunny/crossref_metadata_2025_split huggingface dataset:
Meningeal CGRP‐Prolactin Interaction Evokes Female‐Specific Migraine Behavior
Authors: Avona Amanda , Mason Bianca N. , Burgos‐Vega Carolina , Hovhannisyan Anahit H. , Belugin Sergei N. , Mecklenburg Jennifer , Goffin Vincent , Wajahat Naureen , Price Theodore J. , Akopian Armen N. , Dussor Gregory 
Published: March 2021

ObjectiveMigraine is three times more common in women. CGRP plays a critical role in migraine pathology and causes female‐specific behavioral responses upon meningeal application. These effects are likely mediated through interactions of CGRP with signaling systems specific to females. Prolactin (PRL) levels have been correlated with migraine attacks. Here, we explore a potential interaction between CGRP and PRL in the meninges.MethodsProlactin, CGRP, and receptor antagonists CGRP8‐37 or Δ1‐9‐G129R‐hPRL were administered onto the dura of rodents followed by behavioral testing. Immunohistochemistry was used to examine PRL, CGRP and Prolactin receptor (Prlr) expression within the dura. Electrophysiology on cultured and back‐labeled trigeminal ganglia (TG) neurons was used to assess PRL‐induced excitability. Finally, the effects of PRL on evoked CGRP release from ex vivo dura were measured.ResultsWe found that dural PRL produced sustained and long‐lasting migraine‐like behavior in cycling and ovariectomized female, but not male rodents. Prlr was expressed on dural afferent nerves in females with little‐to‐no presence in males. Consistent with this, PRL increased excitability only in female TG neurons innervating the dura and selectively sensitized CGRP release from female ex vivo dura. We demonstrate crosstalk between PRL and CGRP systems as CGRP8‐37 decreases migraine‐like responses to dural PRL. Reciprocally, Δ1‐9‐G129R‐hPRL attenuates dural CGRP‐induced migraine behaviors. Similarly, Prlr deletion from sensory neurons significantly reduced migraine‐like responses to dural CGRP.InterpretationThis CGRP‐PRL interaction in the meninges is a mechanism by which these peptides could produce female‐selective responses and increase the prevalence of migraine in women. ANN NEUROL 2021;89:1129–1144
Internal plasticization of poly(vinyl chloride) by grafting acrylate copolymers via copper‐mediated atom transfer radical polymerization
Authors: Li Longbo , Schneider Yanika , Hoeglund Adrienne B. , Braslau Rebecca 
Published: March 2021

AbstractInternal plasticization of poly(vinyl chloride) (PVC) was achieved in one‐step using copper‐mediated atom transfer radical polymerization to graft different ratios of random n‐butyl acrylate and 2–2‐(2‐ethoxyethoxy)ethyl acrylate copolymers from defect sites on the PVC chain. Five graft polymers were made with different ratios of poly(butyl acrylate) (PBA) and poly(2–2‐(2‐ethoxyethoxy)ethyl acrylate) (P2EEA); the glass transition temperatures (Tg) of functionalized PVC polymers range from − 25 to − 50°C. Single Tg values were observed for all polymers, indicating good compatibility between PVC and grafted chains, with no evidence of microphase separation. Plasticization efficiency is higher for polyether P2EEA moieties compared with PBA components. The resultant PVC graft copolymers are thermally more stable compared to unmodified PVC. Increasing the reaction scale from 2 to 14 g produces consistent and reproducible results, suggesting this method could be applicable on an industrial scale.
Piperazine and piperidine‐substituted 7‐hydroxy coumarins for the development of anti‐inflammatory agents
Authors: Buran Kerem , Reis Rengin , Sipahi Hande , Önen Bayram F. Esra 
Published: March 2021

AbstractCoumarins (2H‐1‐benzopyran‐2‐one), derivatives that can be isolated from several plants, have been reported for their anticoagulant, antimicrobial, anti‐inflammatory, or anticancer activity. Some of these structures are currently approved for the treatment of cardiovascular diseases, as antibiotics or as an anticancer drug. Given the great potential of this structure and the limited number of studies that focus on molecules derived from carbon 8 of the benzopyranone heterocycle, we synthesized in this project 38 coumarin derivatives by substituting carbon 8 of the benzopyran ring with some aromatic and aliphatically substituted piperidines and piperazines. As a few of these structures were already shown to exhibit some carbonic anhydrase (CA) inhibition and as CA enzymes are reported to be closely related to inflammation, the synthesized derivatives were evaluated for their anti‐inflammatory activity in vitro. The results indicated that compounds 20 and 31 revealed promising anti‐inflammatory activity, as they demonstrated better activity than the reference drugs.
Facile synthesis of C6‐substituted benz[4,5]imidazo[1,2‐a]quinoxaline derivatives and their anticancer evaluation
Authors: Singh Rahul , Kumar Ravinder , Pandrala Mallesh , Kaur Parleen , Gupta Saloni , Tailor Dhanir , Malhotra Sanjay V. , Salunke Deepak B. 
Published: March 2021

AbstractCancer remains a leading cause of death worldwide, resulting in continuous efforts to discover and develop highly efficacious anticancer drugs. High‐throughput screening of heterocyclic compound libraries is one of the promising approaches that provided several new lead molecules with a novel mechanism of action. On the basis of the promising anticancer potential of imidazoquinoxaline as well as the structurally similar imidazoquinoline‐derived scaffold, we prepared a set of C6‐substituted benzimidazo[1,2‐a]quinoxaline derivatives via two novel synthetic routes using commercially available starting materials, with good to excellent yields and evaluated their anticancer activity against the NCI‐60 cancer cell lines. The one‐dose (10 µM) anticancer screening of the synthesized compounds in the NCI‐60 cell line panel revealed that the substituents have a significant role in the activity. In particular, the indole (7f), imidazole (7g), and benzimidazole (7h) derivatives showed significant activity against the triple‐negative breast cancer cell line, MDA‐MB‐468. The lead compounds also exhibited notable IC50 values against another breast cancer cell line, MCF‐7. Furthermore, it was observed that these compounds were relatively nontoxic to normal cell lines: HEK293 (human embryonic kidney cell line) and MCF12A (nontumorigenic human breast epithelial cell line). The IC50 values against healthy cells were at least 5‐ to 11‐fold higher, offering a new class of heterocycles that can be further developed as promising therapeutics for cancer treatment.
Eosinophil ETosis–Mediated Release of Galectin‐10 in Eosinophilic Granulomatosis With Polyangiitis
Authors: Fukuchi Mineyo , Kamide Yosuke , Ueki Shigeharu , Miyabe Yui , Konno Yasunori , Oka Nobuyuki , Takeuchi Hiroki , Koyota Souichi , Hirokawa Makoto , Yamada Takechiyo , Melo Rossana C. N. , Weller Peter F. , Taniguchi Masami 
Published: March 2021

ObjectiveEosinophils are tissue‐dwelling immune cells. Accumulating evidence indicates that a type of cell death termed ETosis is an important cell fate involved in the pathophysiology of inflammatory diseases. Although the critical role of eosinophils in eosinophilic granulomatosis with polyangiitis (EGPA; formerly Churg‐Strauss syndrome) is well established, the presence of eosinophil ETosis (EETosis) is poorly understood. We undertook this study to better understand the characteristics of EETosis.MethodsIn vitro studies using blood‐derived eosinophils were conducted to characterize EETosis. The occurrence of EETosis in tissues from patients with EGPA was studied by immunostaining and electron microscopy. Serum concentrations of eosinophil‐derived proteins in healthy controls, patients with asthma, and EGPA patients with active disease or with disease in remission (n = 15 per group) were examined.ResultsEETosis was reliant on reactive oxygen species and peptidylarginine deiminase type 4–dependent histone citrullination, resulting in the cytolytic release of net‐like eosinophil extracellular traps, free galectin‐10, and membrane‐bound intact granules. The signature of EETosis, including loss of cytoplasmic galectin‐10 and deposition of granules, was observed in eosinophils infiltrating various tissues from EGPA patients. Serum eosinophil granule proteins and galectin‐10 levels were increased in EGPA and positively correlated with disease activity as assessed by the Birmingham Vasculitis Activity Score (r = 0.8531, P < 0.0001 for galectin‐10). When normalized to blood eosinophil counts, this correlation remained for galectin‐10 (r = 0.7168, P < 0.0001) but not for granule proteins. Galectin‐10 levels in active EGPA positively correlated with serum interleukin‐5 levels.ConclusionEosinophils infiltrating diseased tissues in EGPA undergo EETosis. Considering the exclusive expression and large pool of cytoplasmic galectin‐10 in eosinophils, elevated serum galectin‐10 levels in patients with EGPA might reflect the systemic occurrence of cytolytic EETosis.
Recent Advances of Freestanding Cathodes for Li‐S Batteries
Authors: Zhang Peng , Liu Chang , Yang Yadong , Zheng Yang , Huo Kaifu 
Published: March 2021

AbstractLithium‐sulfur batteries (LSBs) with high energy density and low cost have been recognized as one of the most promising next‐generation energy storage systems. Although it has taken decades of development, the practical application of LSBs has been hindered by several inherent obstacles, particularly the severe shuttle effect and sluggish reaction kinetics in the sulfur cathode. Various strategies have been proposed to address these problems via rational design of electrode materials and configurations. Freestanding sulfur cathode could be a promising strategy to improve the sulfur mass loading at the cathode level and energy density of LSBs. This minireview will briefly summary the recent advances in freestanding cathodes for LSBs. The advantages and disadvantages of various freestanding cathodes are discussed and the prospects for the development of flexible cathodes are envisioned.
Paraspeckle nuclear condensates: Global sensors of cell stress?
Authors: McCluggage Finn , Fox Archa H. 
Published: March 2021

AbstractParaspeckles are nuclear condensates, or membranelees organelles, that are built on the long noncoding RNA, NEAT1, and have been linked to many diseases. Although originally described as constitutive structures, here, in reviewing this field, we develop the hypothesis that cells increase paraspeckle abundance as part of a general stress response, to aid pro‐survival pathways. Paraspeckles increase in many scenarios: when cells transform from one state to another, become infected with viruses and bacteria, begin to degenerate, under inflammation, in aging, and in cancer. Cells increase paraspeckles by increasing transcription of NEAT1 and adjusting its RNA processing. These increases in NEAT1 are driven by numerous stress‐sensing signaling pathways, including signaling to mitochondria and stress granules, revealing crosstalk between the cytoplasm and nucleoplasm in the stress response. Thus, paraspeckles are an important piece of the puzzle in cellular homeostasis, and could be considered RNA‐scaffolded nuclear equivalents of dynamic stress‐induced structures that form in the cytoplasm. We speculate that, in general, cells rely on phase‐separated paraspeckles to transiently tweak gene regulation in times of cellular flux.
White paper on high‐throughput process development for integrated continuous biomanufacturing
Authors: São Pedro Mariana N. , Silva Tiago C. , Patil Rohan , Ottens Marcel 
Published: March 2021

AbstractContinuous manufacturing is an indicator of a maturing industry, as can be seen by the example of the petrochemical industry. Patent expiry promotes a price competition between manufacturing companies, and more efficient and cheaper processes are needed to achieve lower production costs. Over the last decade, continuous biomanufacturing has had significant breakthroughs, with regulatory agencies encouraging the industry to implement this processing mode. Process development is resource and time consuming and, although it is increasingly becoming less expensive and faster through high‐throughput process development (HTPD) implementation, reliable HTPD technology for integrated and continuous biomanufacturing is still lacking and is considered to be an emerging field. Therefore, this paper aims to illustrate the major gaps in HTPD and to discuss the major needs and possible solutions to achieve an end‐to‐end Integrated Continuous Biomanufacturing, as discussed in the context of the 2019 Integrated Continuous Biomanufacturing conference. The current HTPD state‐of‐the‐art for several unit operations is discussed, as well as the emerging technologies which will expedite a shift to continuous biomanufacturing.
Investigation of the incompatibility of Knoxiae Radix and Glycyrrhizae Radix et Rhizoma in rats by 1H NMR and MS‐based untargeted metabolomic analysis
Authors: Yan Xu , Zhao Min , Zou Wanru , Tian Pengyao , Sun Lin , Wang Miao , Zhao Chunjie 
Published: March 2021

AbstractKnoxiae Radix (HDJ, usually used after being processed into CHDJ) is a traditional Chinese herbal medicine that has been recorded in the Chinese Pharmacopoeia for many years. It is said that Glycyrrhizae Radix et Rhizoma (GC) is incompatible with HDJ, but this is unproven. In this work, nontargeted metabolomics experiments were performed on rats to evaluate the effect of the combination of the two herbals. For this, we conducted a 28‐day administration in rats. The plasma, urine and kidney tissues were collected for a metabolomics study based on 1H NMR and LC–MS. The OPLS‐DA method was used to screen biomarkers. In addition, the KEGG Pathway database and MetaboAnalyst were used to find metabolic pathways. Twenty‐two significant metabolites were identified. These metabolites were related to many metabolic pathways such as amino acid metabolism, synthesis and degradation of ketone bodies. Significant changes in urine creatinine levels revealed that CHDJ is nephrotoxic. When the GC–CHDJ mass ratio was 1, the toxicity was strengthened; when the GC–CHDJ’ mass ratio was 3, the toxicity was alleviated. This is the first time that a metabolomics approach has been used to investigate the incompatibility of GC–CHDJ.
Symptom clusters experienced by breast cancer patients at various treatment stages: A systematic review
Authors: So Winnie K. W. , Law Bernard M. H. , Ng Marques S. N. , He Xiaole , Chan Dorothy N. S. , Chan Carmen W. H. , McCarthy Alexandra L. 
Published: March 2021

AbstractBreast cancer patients often experience symptoms that adversely affect their quality of life. It is understood that many of these symptoms tend to cluster together: while they might have different manifestations and occur during different phases of the disease trajectory, the symptoms often have a common aetiology that is a potential target for intervention. Understanding the symptom clusters associated with breast cancer might usefully inform the development of effective care plans for affected patients. The aim of this paper is to provide an updated systematic review of the known symptom clusters among breast cancer patients during and/or after cancer treatment. A search was conducted using five databases for studies reporting symptom clusters among breast cancer patients. The search yielded 32 studies for inclusion. The findings suggest that fatigue‐sleep disturbance and psychological symptom cluster (including anxiety, depression, nervousness, irritability, sadness, worry) are the most commonly‐reported symptom clusters among breast cancer patients. Further, the composition of symptom clusters tends to change across various stages of cancer treatment. While this review identified some commonalities, the different methodologies used to identify symptom clusters resulted in inconsistencies in symptom cluster identification. It would be useful if future studies could separately examine the symptom clusters that occur in breast cancer patients undergoing a particular treatment type, and use standardised instruments across studies to assess symptoms. The review concludes that further studies could usefully determine the biological pathways associated with various symptom clusters, which would inform the development of effective and efficient symptom management strategies.
Effectiveness and biochemical basis of wholemeal GlutoPeak test in predicting water absorption and gluten strength of Canadian hard red spring wheat
Authors: Wang Kun , Sangha Jatinder , Cuthbert Richard , Fu Bin Xiao 
Published: March 2021

AbstractBackground and objectivesTo further improve the efficiency of GlutoPeak test for selecting water absorption and gluten strength in wheat breeding programs, this study was conducted to investigate the suitability and effectiveness of wholemeal as testing material by eliminating flour milling process which is often the bottleneck of quality evaluation throughput.FindingsBy analyzing forty‐four advanced wheat lines, strong correlations were found for wholemeal GlutoPeak parameters with conventional flour‐based farinograph absorption (r = .82, p < .001) and stability (r > .76, p < .001), and extensigraph Rmax (r > .90, p < .001). The secondary structures of gluten proteins, collected at different mixing stages of GlutoPeak test, were examined using Fourier transform infrared (FTIR) spectroscopy. The β‐turn structure increased significantly from partially developed to fully developed gluten, indicating its critical role in contributing to gluten viscoelasticity.ConclusionsWithout the preparation of refined flour, wholemeal GlutoPeak test can be a powerful tool for rapid and effective selection of key wheat quality traits.Significance and noveltyThis study demonstrated the effectiveness of wholemeal GlutoPeak test in screening key wheat quality parameters. The changes in gluten protein secondary structure during GlutoPeak test (refined flour or wholemeal) were demonstrated for the first time.
Simple Construction of Amorphous Monometallic Cobalt‐Based Selenite Nanoparticles using Ball Milling for Highly Efficient Oxygen Evolution Reaction
Authors: Zhou Ling‐Li , Pan Dong‐Sheng , Guo Zheng‐Han , Li Jin‐Kun , Huang Sai , Song Jun‐Ling 
Published: March 2021

AbstractAmorphous transition‐metal based materials with disordered structure are very desirable in the field of sustainable energy devices and technologies such as water‐splitting and secondary batteries due to their inherent properties of anisotropy and defects. To date, it is very urgent to explore new methods to prepare amorphous electrocatalysts. Herein, we use a simple approach to construct amorphous Co‐based selenite electrocatalyst by mechanical ball milling for oxygen evolution reaction (OER). Due to its amorphous structure as well as more active species, the obtained amorphous cobalt selenite C4S‐12 h material on the glassy carbon electrode shows superior electrochemical performance with a low overpotential of 255 mV to drive 10 mA cm−2 in 1.0 M KOH electrolyte. In addition, this catalyst also has an excellent intrinsic activity (high mass activity of 162.0 A g−1 and TOF of 0.063 s−1 at the overpotential of 350 mV) and remarkable long‐term stability (at least 32 h at a current density of 10 mA cm−2). This study offers a simple and environmentally friendly method to construct highly efficient electrocatalysts for water oxidation.
Enabling Circular Economy: The Overlooked Role of Inorganic Materials Chemistry
Authors: Salviulo Gabriella , Lavagnolo Maria Cristina , Dabalà Manuele , Bernardo Enrico , Polimeno Antonino , Sambi Mauro , Bonollo Franco , Gross Silvia 
Published: March 2021

AbstractCircular economy is considered a new chance to build a more sustainable world from both the social and the economic point of view. In this Essay, the possible contribution of inorganic chemistry towards a smooth transition to circularity in inorganic materials design and production is discussed by adopting an interdisciplinary approach.
How to Navigate Trainee‐Mentor Relationships and Interpersonal Dynamics in the Lab
Authors: Antes Alison L. , Maggi Leonard B. 
Published: March 2021

AbstractRelationships with mentors and labmates are defining aspects of a researcher's journey in science. Ideally, these interactions are outstanding opportunities to learn from others and provide the basis for lifelong collaborations. Unfortunately, sometimes interpersonal dynamics in the lab are challenging. Graduate students entering the lab can greatly benefit from advice about navigating the interpersonal aspects of doing science. This article covers essential recommendations for developing a good trainee‐mentor relationship and working well with peers in the lab, or being a “good lab citizen.” Lab members–especially graduate students–often spend more time with labmates than with their friends and family during their graduate career, making these relationships essential to their well‐being. The guidance also covers some advice for handling a tense relationship or problematic work environment. Finally, the advice concludes by discussing how to manage the fear of failure, overcome imposter syndrome, develop self‐awareness, and cope with stress. These four issues are fundamental to success in research but are not discussed with graduate students as much as may be necessary. © 2021 Wiley Periodicals LLC.This article was corrected on 25 July 2022. See the end of the full text for details.
Influence of Annealing Temperature Modulation on the Structural and Optical Properties of ZnSe Thin Films
Authors: Yuan Yafei , Liu Chunmin , Li Yaopeng , Li Jing 
Published: March 2021

AbstractThe research work is devoted to study the influence of annealing temperature on the structural and optical properties of ZnSe thin films. The samples are prepared by a thermal evaporation technique and part of the as‐deposited films are annealed. X‐ray diffraction patterns and Raman spectra analysis reveal that the ZnSe thin films have cubic zinc‐blende crystal structure and the crystallinity increases as the annealing temperature increases. For the linear optical parameters, the absorption edge moves to shorter wavelengths with the increase of annealing temperature. Based on Swanepoel's method, the refractive index, film thickness, absorption coefficient and optical band gap are determined. The values of band gap are found to increase from 2.63 to 2.68 eV as the annealing temperature increases. By performing an open aperture Z‐scan technique with femtosecond pulses under 800 nm wavelength, all ZnSe samples show two‐photon absorption responses. It is found that the modulation depth and the two‐photon absorption coefficient decrease with increasing annealing temperature. Moreover, all samples exhibit better optical limiting responses at higher incident power intensities. These novel properties render this a promising material for traditional optoelectronic devices and optical limiters.