bluuebunny/crossref_metadata_2025_split huggingface dataset:
Tissue Expression of Methyltransferases in Response to Acute and Long-term Exercise in Sedentary Men
Authors: Olsen Thomas , Lee Sindre , Vinknes Kathrine , Norheim Frode , Hjorth Marit , Turner Cheryl , Refsum Helga , Jensen Jørgen , Birkeland Kåre , Drevon Christian 
Published: January 2021

Abstract
Exercise influences epigenetic regulation of gene expression by modulating tissue methyltransferase activity, whereas effects on methyltransferases with other crucial biological functions have not been elucidated. We performed RNA sequencing of skeletal muscle (SkM) and white adipose tissue (WAT) obtained from 26 sedentary men undergoing acute exercise (AE) and a long-term, 12-week exercise intervention (LTE). We investigated exercise effects on tissue methyltransferase transcripts and a plasma marker of methylation capacity (methionine/homocysteine ratio). Blood and tissue samples were obtained before, just after and 2 h after AE (blood and SkM), and before and after LTE (blood, SkM, WAT). Differential expression analyses revealed that 43 (15 up; 26 down) and 55 (31 up; 23 down) methyltransferases were differentially expressed in SkM just after and 2 h after AE, respectively. After LTE, 69 methyltransferases (13 up; 55 down) were differentially expressed in SkM. Upregulated methyltransferases were implicated in histone and peptidyl-lysine methylation (AE, 0 h), RNA processing (AE, 2 h), and cell communication (LTE). Downregulated methyltransferases were implicated in gene expression (AE, 0 h) and mRNA processing (LTE). Plasma methionine/homocysteine decreased after AE, but was elevated after LTE. In conclusion, AE and LTE influence SkM but not WAT methyltransferase transcript levels and plasma methionine/homocysteine.
Predictive Value of Cerebrospinal Fluid Biomarkers for Tap Test Responsiveness in Patients With Suspected Idiopathic Normal Pressure Hydrocephalus
Authors: Hua Rongrong , Liu Chunyan , Liu Xing , Zhu Jinwu , Zhang Jie , Wang Lidong , Shi Zhe , Li Jian , Kong Shuangyan , Yang Chenhui , Liu Nan , Liu Lijuan , Sun Jie , Yang Qiong , Wu Yubing , Zhou Ying , Li Yanfeng , Xing Yan 
Published: January 2021

Abstract
Background The value of cerebrospinal fluid (CSF) biomarkers for idiopathic normal pressure hydrocephalus (iNPH) needs to be determined. This prospective study aimed to reveal the correlation between CSF biomarkers and clinical symptoms of iNPH, and its predictive value for tap test responsiveness.Case presentation Thirty-nine suspected iNPH patients were recruited, contributed qualified CSF, and accepted a tap test and unified pre- and post-test evaluation of neurological function. The analysis of biomarkers from their CSF showed a decrease of tau and its phosphorylated form, especially in the tap test (+) group. In addition, the responsiveness of the tap test was also related to the number of combined symptoms (p<0.01). A correlation was also found between the end pressure or pressure difference of CSF and tap test responsiveness (p<0.05). The results of binary logistic regression analysis showed that P (tap test responsiveness) = 1/1 + e ^ - (-5.505+55.314 * ratio of p/T-tau - 1.586 * numbers of combined symptoms). The combined indicators (-5.505+0.553* percentage of p/T-tau - 1.586 * numbers of combined symptoms) gave the highest sensitivity and specificity, which were 94.12% and 72.73%, respectively. Conclusions The combined indicators calculated from p/T-tau and the numbers of combined symptoms may be accessed in judgment of tap test responsiveness, which is beneficial for the feasibility of clinical application.
Expression of lncRNAs Associated with GPCRs and Clinical Prognosis in GBM
Authors: Wang Hao , Wang Yaohui , xiang wei 
Published: January 2021

Abstract
Background: Glioblastoma (GBM) is a primary malignant tumor of the central nervous system with a poor prognosis. Long non-coding RNAs (lncRNAs) play a variety of key regulatory roles in a variety of biological processes, and have an important influence on the occurrence and development of tumors by regulating the expression of target genes. However, their role in the prognosis of GBM is still lacking in accurate prognostic markers.This study aims to establish an effective lncRNAs model to evaluate the prognosis of GBM.Methods: We used data of mRNA, lncRNA and clinical follow-up from The Cancer Genome Atlas (TCGA) to conduct univariate analysis, clustering analysis, coding gene difference analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis and Gene Ontology (GO) analysis for GBM patients. lncRNAs that are closely related to the survival and prognosis of GBM were found and a multiple regression model was constructed to calculate the risk score of the samples, so as to accurately predict the clinical prognosis of GBM patients.Results: Through multiple systematic analysis, we found 5 lncRNAs that are closely related to the survival and prognosis of GBM, and these 5 lncRNAs can be used as independent prognostic factors. Through GO enrichment analysis and KEGG pathway analysis, we found that there is a close relationship between GBM and G protein coupled receptors. Therefore, 93 mRNAs associated with G-protein-coupled receptors and 5 lncRNAs associated with independent prognostic factors were selected to calculate the correlation, respectively. In other words, a tumor-specific lncRNAs/mRNAs co-expression network was constructed through biological prediction based on correlation analysis. The results showed that lncRNAs and mRNAs interregulated very closely in GBM patients. In addition, we also constructed a multiple regression model with these 5 lncRNAs which was able to calculate the risk score of each sample to accurately predict the clinical prognosis of GBM patients.Conclusion: Our study found lncRNAs independently were related to the prognosis of GBM, and successfully constructed a multiple regression model related to lncRNAs, providing a new perspective for better evaluation of the role of lncRNAs in the clinical prognosis of GBM.
The Association Between Deficiency of Nutrient Intake on Resting Metabolic Rate in Overweight and Obese Women: a Cross-sectional Study
Authors: Sajjadi Seyedeh Forough , Mirzababaei Atieh , Abdollahi Afsoun , Shiraseb Farideh , Mirzaei Khadijeh 
Published: January 2021

Abstract
Objective: The double burden of malnutrition is an emerging public health concern nowadays which a correlation with Obesity. This study aimed to examine the relationship between resting metabolic rate (RMR) and dietary intake of zinc, vitamin C, and riboflavin in overweight and obese women.Results: the RMR/FFM showed a significant association with riboflavin (β=1.59; 95% CI: 1.04-23.26, P=0.04) and zinc (β=0.78; 95% CI: 1.04-4.61, P=0.03) in the crude model. Moreover, differences in vitamin C and RMR/FFM was marginal significant (β=0.75; 95% CI: 0.95-4.77, P=0.06). After adjusting for confounders the riboflavin association change to marginal significance (β=1.52; 95% CI: 0.91-23.04, P=0.06). After controlling for potential confounders, the associations change between zinc and RMR/FFM (β=0.66; 95% CI: 0.78-4.86, P=0.15) and between RMR/FFM and vitamin C (β=0.48; 95% CI: 0.66-3.96, P=0.28). Our study showed a significant association between dietary intake of zinc, riboflavin, and vitamin C and change in RMR/FFM in overweight and obese women.
Do Assessments of Cardiorespiratory and Muscular Fitness Influence Subsequent Reported Physical Activity? A Randomized Controlled Trial.
Authors: Langland James T , Sathnur Neeraj , Wang Qi , Olson Andrew PJ 
Published: January 2021

Abstract
BackgroundClinicians strive to motivate healthy lifestyle behaviors such as exercise. Cardiorespiratory fitness (CRF) and muscular strength are important for good health and their measurement could potentially provide motivation to exercise. We investigated if measurements of CRF and muscular fitness (MF) would influence subsequent self-reported physical activity. MethodsVolunteer subjects at a State Fair were randomized in 1:1 parallel fashion to control and intervention groups. The baseline Exercise Vital Sign (EVS) and type of physical activity were obtained from all subjects. The intervention group received estimated maximum oxygen uptake (VO2max) using a step test and muscular strength using a hand grip dynamometer with age-specific norms for both measurements. All subjects were provided exercise recommendations and follow up surveys during the following year regarding their EVS and physical activity. Results1315 individuals (656 intervention, 659 control) were randomized with one year follow up data obtained from 823 subjects (62.5%). Baseline mean EVS was 213 min/week. No change in EVS was found either group at follow-up (p=0.99). Subjects who were less active at baseline (EVS<150) did show an increase in EVS (86 to 146) at 6 months (p<0.05). At 3 months the intervention group increased resistance training (29.1% to 42.8%) compared to controls (26.3% to 31.4%) (p<0.05). Lifestyle physical activity increased in the intervention group at 3 months (27.7% to 29.1%) and 6 months (25%) whereas it declined in the control group at 3 months (24.4% to 20.1%) and 6 months (18.7%) (p<0.05). ConclusionProviding VO2max estimates and grip strength did not produce an increase in overall physical activity. The EVS and exercise recommendations did however result in increased physical activity in less active individuals. In a very active population the VO2max estimate and measured grip strength did increase lifestyle activity and resistance training. Wider adoption of these measures could be effective in promoting physical activity and resistance training.Trial Registration Numberclinicaltrials.gov NCT03518931 Registered 05/08/2018 -retrospectively registered https://clinicaltrials.gov/ct2/results?cond=&term=NCT03518931&cntry=&state=&city=&dist=
Bioactive Glass Synthesized by the Acid-free Hydrothermal Method
Authors: Tuan Ta Anh , Guseva Elena V. , Tien Nguyen Anh , Long Nguyen Viet , Phuc Le Hong , Hien Nguyen Quan , Hiep Dang Tan , Vi Vo Thuy , Hoa Bui Thi , Ha Bui Manh , Vuongi Bui Xuan 
Published: January 2021

Abstract
Ternary bioactive glass 58SiO2-33CaO-9P2O5 (wt.%) was elaborated by the acid-free hydrothermal method. Thermal behavior, textural property, phase composition, morphology, and ionic exchange were investigated by thermal analysis, N2 adsorption/desorption, XRD, FTIR, SEM, and ICP-OES analysis. The bioactivity and biocompatibility of synthetic bioactive glass were evaluated by in vitro experiments with SBF solution and cell culture medium. The obtained results confirmed that the acid-free hydrothermal process is one of the standard methods for preparing bioactive glass.
Analysis of The Influence of The Critical Speed of Thermoelastic Instability of Transfer Case on The Torque Transmission
Authors: WANG Yu Ming , CHEN Liqing , LIANG Xiutian , LI Hongliang 
Published: January 2021

Abstract
Based on the thermoelastic instability theory, the variation trend of film thickness on the surface of transfer case friction pair is studied, and the basic process in which the thickness of lubricating oil film changes due to thermoelastic instability was obtained. According to this process, the critical speed of transfer case friction pair is used to reflect transfer torque stability. Based on above, firstly, the dynamic simulation model of the transfer case is established from which the dynamic characteristic equation of the transfer case under the combined action of asperity torque and viscous torque is obtained. Secondly, according to the result that the thickness of the transfer case lubricating oil film is affected by the joint action of lubricating oil pressure, contact pressure and thermal expansion, the condition of thermoelastic instability of the transfer case torque transmission is discussed. The result shows that the critical speed of the thermoelastic instability increases correspondingly with the increase of the thickness of the oil film. The critical curve divides the engagement process into the stable region at the upper part of the curve and the unstable region at the lower part of the curve, so as to calculate the thermoelastic instability region of the torque transfer of the transfer case. Finally, in order to further analyze the influence of thermoelastic instability on the characteristics of the transfer case, three factors, namely the surface roughness of the friction plate, the lubricating oil viscosity and the thermal conductivity of the dual steel disc, are mainly selected for analysis, and the thermoelastic instability is taken into account. The conclusion is expected to be used in the engineering practice of torque distribution control of the transfer case.
Minocycline Attenuation Of Rat Corpus Callosum Abnormality Mediated By Low Dose Lipopolysaccharide-induced Microglia Activation
Authors: Xiong Huangui , Zhang Jingdong , Zheng Ya , Liu Jianuo , Fox Howard S. 
Published: January 2021

Abstract
Background: Microglia are resident innate immune cells in the brain and the activation of these myeloid cells results in secretion of a variety of proinflammatory molecules, leading to the development of neurodegenerative disorders. Lipopolysaccharide (LPS) is a widely used experimental stimulant in microglia activation. We have previously shown that LPS produced microglia activation and evoked detectable functional abnormalities in rat corpus callosum (CC) in vitro. Here, we further examined the effects of low dose LPS-induced microglia activation and resultant white matter abnormality in the CC in vivo and its attenuation by an anti-inflammatory agent minocycline.Methods: Twenty-four SD rats were divided randomly into three groups and intra-peritoneally injected daily with saline, LPS and LPS+minocycline, respectively. All animals were subject to MRI tests 6 days post injection. The animals were then sacrificed for harvest the CC tissues for electrophysiology, Western blotting, and immunocytochemistry. One-way ANOVA with Tukey’s posttest of all pair of columns was employed statistical analyses.Results: Systemic administration of LPS produced microglial activation in the CC as illustrated by Iba-1 immunofluorescent staining. We observed that a large number of Iba-1 positive microglial cells were hyper-ramified with hypertrophic somata or even amoeba like in the LPS-treated animals and such changes were significantly reduced by co-administration of minocycline. Electrophysiological recordings of axonal compound action potential (CAP) revealed an impairment on the CC functionality as detected by a reduction on CAP magnitude. Such an impairment was supported by a reduction on fast axonal transportation evidenced by β-amyloid precursor protein accumulation. These alterations were attenuated by minocycline, demonstrating minocycline reduction of microglia-mediated interruption of white matter integrity and function in the CC. Conclusions: Systemic administration of LPS produced microglia activation in the CC and resultant functional abnormalities that were attenuated by an anti-inflammatory agent minocycline.
The association between cumulative dose of inhaled bronchodilators and mortality in patients with severe ARDS
Authors: Scott Ashley M. , Fadel R.A. , Asala E.D. Abou , Murskyi I.O. , Bachler J.L. , Ouellette Daniel R. 
Published: January 2021

Abstract
Background: The purpose of this study was to examine bronchodilator use in patients with severe acute respiratory distress syndrome (ARDS) to determine if such use affected mortality. We compared cohorts of patients receiving and not receiving bronchodilators to determine clinical differences between the groups. We then sought associations with clinical risk factors, including cumulative doses of bronchodilators, with in-hospital mortality.Methods: This study was a retrospective analysis of patients diagnosed with severe ARDS treated at a large health care system between December 1, 2012 and February 10, 2018. From 1195 patients selected from the electronic medical records (EMR) we identified 312 patients for study after the application of inclusion and exclusion criteria. We compared cohorts of patients receiving and not receiving inhaled bronchodilators. We then performed first a univariable, followed by a multivariable analysis of clinical risk factors including the cumulative dose of bronchodilators to study their relationship with in-hospital mortality. Results: Of the 312 patients with moderate or severe ARDS selected for this study, 260 received bronchodilators and 52 did not receive them. Most patients (n=230) received both albuterol and ipratropium. Patients receiving bronchodilators had longer intensive care unit length of stay (ICULOS; 18.6+/-14.9 days v 9.0+/-7.4 days, p=0.000) but similar mortality compared to patients not receiving bronchodilators (112/260 versus 26/52, p=0.364). Clinical risk factors significantly associated with in-hospital mortality in a univariable analysis included the mortality prediction model II score (MPM, p=0.000), age (p=0.000), the ratio of PaO2:FiO2 (PF, p=0.000), the cumulative amount of short acting bronchodilator (SABD, p=0.004), the maximum heart rate (HRmax, p=0.023), and the minimum serum potassium level (Kmin, p=0.000). In a multivariable analysis the MPM (p=0.000, OR=7.543), PF (p=0.002, OR=0.988), cumulative SABD dose (p=0.002, OR=0.996), HRmax (p=0.001, OR=1.021), and Kmin (p=0.000, OR=3.509) remained significantly associated with inpatient mortality. Conclusions: Inhaled bronchodilators are frequently used in patients with severe ARDS despite clinical evidence that beta-agonists do not improve clinical outcome. We have demonstrated an association between the cumulative dose of SABD and decreased in-hospital mortality in patients with severe ARDS. Further study is needed to confirm these observations.
Sequential blood lactate measurement as a predictor of mortality in patients with sepsis in the critical care unit
Authors: Borah Pollov , Saloi Dilip Kumar , Deka Bipul , Konwar Ranjumoni , Kalita Deepjyoti , Mahanta Putul , Deka Sangeeta , Phukan Chiranjita 
Published: January 2021

Abstract
Background and objectiveIn severe sepsis, increased blood lactate levels are observed indicating impaired oxidative phosphorylation, which secondarily causes hypoxic hypoxia and stagnant tissue hypoxia. Among all other related factors, a high rise of the lactate level in blood may be a useful predictor of sepsis patients' mortality. This study aims to determine the association of consecutive blood lactate levels with the patient's mortality with sepsis admitted in the Critical Care Unit (CCU). MethodsThis prospective study included 50 patient of septicemia at CCU above 18 years in a tertiary care centre. On admission, at 24 hours and 72 hours, blood lactate levels were monitored. Their clinical status was evaluated for 28 days to categorise as survivor and non-survivor. The statistical analysis was made with Microsoft Excel and SPSS version 20. To test the difference in mean blood lactate levels among survivors and non-survivors Student’s t-test was applied. A p-value of less than 0.05 was considered significant. Prior ethical clearance from the institutional ethics committee of human with informed consent from the patients was obtained for data collection.ResultsThe current study included 50 patients of septicemia, 23 were survivors, and 27 were non-survivors after 28 days of follow up. The mean lactate range for the 23 survivors was ranged from 0.43 mmol/l to 5.69 mmol/l, whereas for non-survivors, it was 1.64 mmol/l to 6.14 mmol/l. The mean value of lactate for the survivors and non-survivors during admission, at 24 hours and at 72 hours were 0.9545±0.45798 vs 2.5204±1.51498, 1.2461±1.21360 vs 2.5107±1.63678 and 1.5496±1.66788 vs 2.7904±2.00160. The differences between the mean lactate values between survivors and non-survivors at different time intervals were highly significant.ConclusionA slower rate of lactate clearance during hospitalisation may be a significant factor associated with severe sepsis patient mortality. Thus serial blood lactate levels is a significant predictor of mortality and should be monitored.
Evaluation of Mobile Supervision Application and Electronic Medicine Box for Tuberculosis Patients: Implementation Experience from Tianjin During 2019-2020
Authors: Li Xiaorong , pang Xuewen , Zhang Fan 
Published: January 2021

Abstract
Backgroud Poor tuberculosis (TB) medication adherence increases the risk of treatment failure and development of drug-resistant TB, while universal implementation of directly observed therapy (DOT) is not feasible in China. EHealth technologies were reported to be promising patient-centered tools for improving adherence. However, only pilot studies have assessed patients’ experiences and the results were discrepant. Methods This cross sectional study study was conducted among TB patients at the outpatient department from 3 March 2019 to 30 May 2020 in Tianjin, China. Data were downloaded from the Tuberculosis doctor App and TB Information Management System (TBIMS), and merged them using the TBIMS notification number. Logistic regression analysis was used to analyze the factors associated with regualar drug-intake. Odds ratios and 95% confident intervals were estimated with and without adjustment for age, gender, ethnicity and occupation. Results A total of 1193 TB patients were recruited, of whom 33.28% (397) patients were regular drug-intake. The whole drug-intake rate was 84.84%, except for the first month, the drug-intake rate decreased with the increase of monthly treatment sequence. After adjustment by age, gender, ethnicity and occupation, tuberculosis pleurisy (aOR: 0.42, 95CI%=0.26-0.69) and retreated patients (aOR: 0.40, 95CI% =0.27-0.59) were more likely to have poor medication compliance. Local resident tend to have better medication compliance (aOR: 1.80, 95CI% =1.16-2.79).Conclusions The medication adherence at the first month should be noticed. Medication adherence was poor in tuberculosis pleuritis and retreated patients, while local resident tend to have better medication adherence. To make full use of the application of eHealth in TB patient management, more incentive measures should be adopted for patients and doctors respectively.
Presence of secondary bladder cancer following radical nephroureterectomy for upper tract urothelial carcinoma: Characteristics, Risk Factors, and Predictive Value
Authors: Lai Shicong , Wu Pengjie , Liu Shengjie , Seery Samuel , Liu Jianyong , He Lei , Liu Ming , Zhang Yaoguang , Wang Jianye 
Published: January 2021

Abstract
Objective

To assess the characteristics, predictive risk factors, and prognostic effect of secondary bladder cancer (BCa) following radical nephroureterectomy (RNU) in upper tract urothelial carcinoma (UTUC).
Methods

Using the Surveillance, Epidemiology, and End Results (SEER) database, the authors analyzed clinicopathologic characteristics and survival data from 472 UTUC patients with secondary BCa after RNU between 2004 and 2017. Cox’s proportional hazard regression model was implemented to identify independent predictors associated with post-recurrence outcomes. The threshold for statistical significance was p < 0.05.
Results

In total, 200 Ta-3N0M0 localized UTUC patients with complete data were finally included. With a median follow-up of 71 months (interquartile ranges [IQR] 36 -103.75 months), 52.5% (n = 105) had died, with 30.5% (n = 61) dying of UTUC. The median time interval from UTUC to BCa was 13.5 months (IQR 6–40.75 months). According to multivariable Cox regression analysis, patients with intravesical recurrence (IVR) located at multiple sites, advanced BCa stage, higher BCa grade, elderly age and a shorter recurrence time, encountered worse cancer-specific survival (CSS) (all p ༜0.05).
Conclusions

For primary UTUC patients experiencing IVR after radical surgery, advanced age, multiple IVR sites, shorter recurrence time, higher BCa stage, and grade proved to be significant independent prognostic factors of CSS. We ought to pay more attention to IVR prevention as well as to earlier signs which may increase the likelihood of early detection. Having the ability to manage what may be seen as the superficial BCa signs may enable us to improve survival but further research is required.