bluuebunny/crossref_metadata_2025_split huggingface dataset:
Diversity and composition of soil bacterial community respond to A. palmeri invasion under heterogeneous habitats
Authors: Zhang Mei , Han Jianhua , Li Xueying , Zheng Pufan , Qiu Zhenlu , Tang Lili , Wang Tongtong , Shi Fuchen 
Published: June 2021

Abstract
The impact of A. palmeri invasion on soil bacterial community under different habitats is unclear. In this work, the influence of A. palmeri invasion on soil bacterial diversity and community structure were investigated using full-length 16S rRNA sequencing technology under four typical habitats of riverbank (A), roadside (B), wasteland (C) and farmland (D). A two-way ANOVA analysis showed that habitat, invasion and the interaction of them had little effect on alpha diversity, expect for habitat factor had a significant effect on Simpson indices (P<0.05). NMDS analysis demonstrated that soil bacterial community structures among different invasive habitats were clearly distinguished. In addition, the most abundant phyla in the non-invasive plots were Proteobacteria, Planctomycetes and Gemmatimonadetes. However, the third predominant phyla converted from Bacteroidetes to Gemmatimonadetes with the invasion of A. palmeri. LEfSe analysis revealed that the core microbiome, Burkholderiaceae and Betaproteobacteriales (riverbank habitat), Gemmatimonadetes and Gemmatimonadaceae (wasteland habitat), Sphingomonas_sediminicola (roadside habitat), Nitrosomonadaceae (farmland habitat), which played important roles in facilitating the establishment of A. palmeri to heterogeneous habitats.
Gene Clusters Based on OLIG2 and CD276 Could Distinguish Molecular Profiling in Glioblastoma
Authors: Fu Minjie , Zhang Jinsen , Li Weifeng , He Shan , Zhang Jingwen , Tennant Daniel , Hua Wei , Mao Ying 
Published: June 2021

Abstract
BackgroundThe molecular classification of glioblastoma (GBM) based on transcriptomic analysis could provide precise treatment and prognosis. However, current subtyping (Classic, Mesenchymal, Neural, Proneural) is a time-consuming and cost-intensive process, which hinders its clinical application. A simple and efficient method for classification was imperative.MethodsRandom forest algorithm was applied to conduct a gene cluster featured with hub genes, OLIG2 and CD276. Functional enrichment analysis and Protein-protein interaction were performed using the genes in this gene cluster. The classification efficiency of the gene cluster was validated by WGCNA and LASSO algorithms, and tested in GSE84010 and Gravandeel’s GBM datasets. ResultsThe gene cluster (n = 26) could distinguish mesenchymal and proneural excellently (AUC = 0.92), which could be validated by multiple algorithms (WGCNA, LASSO) and datasets (GSE84010 and Gravandeel’s GBM dataset). The gene cluster could be functionally enriched in DNA elements and T cell associated pathways. Additionally, five genes in the signature could predict the prognosis well (p = 0.0051 for training cohort, p = 0.065 for test cohort). Conclusions﻿This study proved the accuracy and efficiency of random forest classifier for GBM subtyping and provided a convenient and efficient method for subtyping Proneural and Mesenchymal GBM.
Revitalising Embodied Community Knowledges as Leverage for Climate Change Engagement
Authors: Donkers Laura 
Published: June 2021

Abstract
Scientists inform us that the industrialised epoch has caused climate breakdown and ecological collapse that threaten human survival. These facts impose a sense of urgency on us to understand that our modern ways of living have created these problems and that we must find ways to resolve them. Communities need to come to terms with the damage that industrialisation has caused to ecology, adapt to the climatic consequences that have been unleashed, and adopt more sustainable ways of living. Yet, the scale of this crisis and lack of wisdom to act can be numbing, so how can communities become more informed and motivated to take action? This paper proposes a performative, practice-led approach to generate discrete engagement strategies that enliven public attention towards changing attitudes, developing more holistic behaviours and adopting mitigative actions. Such engagements are relatable, values-oriented and framed towards the priorities, knowledges, capacities, and lived experiences of rurally-based, intergenerational communities. This novel approach is exemplified in a case study on community food growing that successfully achieved decarbonisation targets. Public engagement was developed through the strategic promotion of embodied community knowledges (ECK) to engender and intensify a sense of ability to respond and act. A series of decarbonisation projects were designed to engage and mobilise local participation through the practical, adaptive capacities that lay within the community. Findings revealed that valuing a community’s existing body of knowledge and revitalising that knowledge as a method of climate change engagement increased the potential for attentive, effective, and committed community-based actions.
Efficacy of Aflibercept for DME with Different OCT Types
Authors: Tao Yuan , Kou Mingrong , Yang Xia , Li Yixiao , Li Shuting , Zhao Hui , Wang Hong , Li Weiguo 
Published: June 2021

Abstract
Background: This study aimed to assess efficacy differences of aflibercept by intravitreal injection for diabetic macular edema (DME) with different OCT types. Methods: according to OCT morphology, 128 patients (128 eyes) with DME were divided into the diffuse retinal thickening (DRT; 41 patients, 41 eyes), cystoid macular edema (CME; 47 patients, 47 eyes), and serous retinal detachment (SRD; 40 patients, 40eyes) groups. All patients were treated by intravitreal injection of 0.5 mg (0.05 mL) aflibercept according to the 3+prn regimen. BCVA (LogMAR) and central foveal thickness (CFT) among the three groups at baseline, and 1 month, 3 months, 6 months and 12 months after treatment, respectively, were assessed. Results: At 1 month, 3 months, 6 months and 12 months after treatment, improvements in BCVA and CFT in the three groups showed statistical significance (P<0.05). BCVA improvement was better at all time points in the DRT group compared with the SRD group (P<0.05). BCVA improvement was also better in the CME group compared with the SRD group at 6 months and 12 months post-treatment (P<0.05). At 1 month, 3 months and 6 months after treatment, CFT differences among the three groups had no statistical significance (P>0.05), while at 12 months post-treatment, CFT values among the three groups had significant differences (F=3.77, P=0.0269). at 12 months after treatment, CFT improvement was better in the DRT and CME groups compared with the SRD group (P<0.05). Conclusion: aflibercept by intravitreal injection could significantly improve visual acuity in DME patients with different OCT types and reduce CFT. At the initial treatment of 12 months, CFT and BCVA improvements in the DRT and CME types were the most pronounced.
Potential Impacts of Polymetallic Nodule Removal On Deep-Sea Meiobenthos
Authors: Pape Ellen , Bezerra Tania , Gheerardyn Hendrik , Buydens Marius , Kieswetter Amanda , Vanreusel Ann 
Published: June 2021

Abstract
Deep seabed mining is imminent in the Clarion Clipperton Fracture Zone (CCFZ; northeast Pacific). Seabed collectors will remove the polymetallic nodules and the surrounding surface sediments, both inhabited by meiobenthos, along their path. To determine potential impacts of polymetallic nodule removal, we investigated the importance of nodule presence for sediment abundance, composition and diversity of meiobenthos, nematodes and copepods, and evaluated the existence and composition of nodule crevice meiobenthos in the Global Sea Mineral Resources (GSR) exploration contract area. Nodule-free and nodule-rich sediments displayed high biodiversity with many singletons and doubletons, potentially representing rare taxa. Nodule presence negatively influenced sediment meiobenthic abundances but did not markedly affect community composition or diversity. This is the first report on CCFZ nodule crevice meiobenthos, whose abundance related positively to nodule dimensions. Though dominated by the same taxa, the meio- and nematofauna differed between sediments and nodules regarding community and functional composition. Nevertheless, there were no taxa endemic to the nodule crevices and nodule crevice meiobenthos added only little to total small-scale (~ cm) meiobenthic abundance and diversity. We formulated environmental management recommendations at the contract area and regional (CCFZ) scale related to sampling effort, set-aside preservation and monitoring areas, and potential rehabilitation measures.
Synthesis of Copper Nanoparticles on Cellulosic Fabrics and Evaluation of their Multifunctional Performances
Authors: Sharma Veerender , Basak Santanu , Ali Wazed 
Published: June 2021

Abstract
Two different kinds of copper nanoparticles (CuNPs) (brown colour and greenish colour) were synthesised by using simple solution route and applied on cotton by exhaust method to achieve multidimensional functionalization on one of the most popular cellulose materials e.g., cotton fabric. The synthesised CuNPs imparted different colours to cotton textile due to different conditions of synthesis and localized surface plasmon resonance. Physico-chemical characterizations of the synthesized nanoparticles were performed by using scanning electron microscope (SEM) and EDX analysis whereas the optical properties of the nanoparticles were studied using UV-visible spectroscopy. The prepared CuNPs of both the types demonstrated very good antimicrobial activity up to 97%. In addition, cotton fabric treated with CuNPs showed very high catalytic activity for reduction of 4-nitroaniline in presence of sodium borohydride to phenylene diamine. Washing durability and rubbing fastness of the treated fabric have also been measured by following standard testing methods and found to be very good with a rating 4.
Pulse Wave Velocity as a Measure of Arterial Stiffness in Patients with Abdominal Aortic Aneurysm: A Systematic Review and Meta-Analysis
Authors: Chen Xiaofeng , Zhang Hongliang , Shi Yangyang , Chen Tingting , Zhang Yang , Wu Juntao , Weng Yekai , Zhao Wengjun , Chen Guang , Li Danlei , Fang Xiaoxin , Xu Cheng , Xu Baohui , Jiang Jianjun 
Published: June 2021

Abstract
Background and objective:

The relationship between pulse wave velocity (PWV) levels and abdominal aortic aneurysm (AAA) remains controversial. A meta-analysis was performed to establish whether vascular pulse wave velocity (PWV) as a measure of arterial stiffness is different in patients with abdominal aortic aneurysms and controls.
Methods

Pubmed, Embase, Cochrane and China National Knowledge Infrastructure (CNKI) were used for the meta-analysis with articles up to January 1, 2021. To compare PWV levels between AAA patients and healthy controls, pooled weighted mean difference (WMD) and its 95% confidence interval (Cl) were calculated. Subgroup analysis and funnel plots are used to assess the quality of the combined results to ensure a normal distribution of data with minimal bias. Study quality for eligible studies was assessed using the Agency For Health Care Research and Quality (AHRQ) inventory tool.
Results

Nine cross-sectional studies, which included 439 abdominal aortic aneurysm cases and 382 healthy subjects, met inclusion criteria and were eligible for meta-analysis. We found that PWV levels were significantly higher [WMD(95%Cl): 2.36(2.02,2.70)] in AAA patients than healthy controls. After subgroup analysis, it was found that age, sex, smoking and hypertension had significant effects on the PWV levels. The normal distribution of the Funnel plot analysis suggests a low risk for publication bias.
Conclusion

PWV levels were elevated in patients with AAA compared to healthy controls, with the effect on PWV altered by age, sex, smoking and hypertension. Our study suggests that abdominal aortic aneurysm is related to increased arterial stiffness.
Inverse Airfoil Design Method for Generating Varieties of Smooth Airfoils Using Conditional WGAN-GP
Authors: Yonekura Kazuo , Miyamoto Nozomu , Suzuki Katsuyuki 
Published: June 2021

Abstract
Machine learning models are recently utilized for airfoil shape generation methods. It is desired to obtain airfoil shapes that satisfies required lift coefficient. Generative adversarial networks (GAN) output reasonable airfoil shapes. However, shapes obtained from ordinal GAN models are not smooth, and they need smoothing before flow analysis. Therefore, the models need to be coupled with B'ezier curves or other smoothing methods to obtain smooth shapes. Generating shapes without any smoothing methods is challenging. In this study, we employed conditional Wasserstein GAN with gradient penalty (CWGAN-GP) to generate airfoil shapes, and the obtained shapes are as smooth as those obtained using smoothing methods. With the proposed method, no additional smoothing method is needed to generate airfoils. Moreover, the proposed model outputs shapes that satisfy the lift coefficient requirements.
Comparing the Efficacy of Sinus Irrigation with Traditional Caldwell-Luc Procedure Following Odontogenic Cyst Surgery Involving The Maxillary Sinus
Authors: Gang Niu , JiaMin Chen , Ye Wu , FuPing Xie , HuangHuang Liu , LiSong Lin 
Published: June 2021

Abstract
Objective: A large maxillary odontogenic cyst could intrude into the maxillary sinus. The traditional approach following surgery for such a cyst is the Caldwell-Luc procedure However the traditional CDL procedure is associated with more complications and damage of the sinus mucosa. The purpose of this study was to assess a new method with easier operation, which not only prevented postoperative infection but also caused less damage to the maxillary sinus mucosa.Methods: A large odontogenic cyst in the maxillary sinus of 40 patients was diagnosed through radiographic imaging and postoperative histopathology. Twenty patients were treated with maxillary sinus saline irrigation after cyst resection surgery, while 20 patients underwent the traditional Caldwell-Luc procedure. The therapeutic efficacy was evaluated by clinical examination and radiographic imaging; the longest follow-up was 36 months.Results: The postoperative recovery of the 20 patients treated with sinus irrigation. Six patients had mild swelling (30%), 12 had moderate swelling (60%), and 2 had severe swelling (10%); 16 had mild pain (80%), 4 had moderate pain (20%), and 0 had severe pain (0%). Among the 20 patients treated by the Caldwell-Luc approach. Immediately postoperatively, 2 patients had mild swelling (10%), 12 had moderate swelling (60%), and 6 had severe swelling (30%); 10 had mild pain (50%); 6 had moderate pain (30%), and 4 had severe pain (20%). On the second day after surgery, a mild temperature elevation (38.0°C-39.0°C) was noted in 8 patients (40%) treated with the Caldwell-Luc procedure and 1 patient (5%) treated with saline irrigation.Conclusion: Sinus irrigation following large odontogenic cyst surgery involving the maxillary sinus can serve as an alternative to standard CDL and has the advantages of fewer complications, reduced trauma, restoration of the mucosa and more satisfactory results.
Study of Compensatory Strategies for Reach-to-Grasp-Pen in Stroke Patients
Authors: XIE Qiurong , Sheng Bo , Huang Jia , Zhang Yanxin 
Published: June 2021

Abstract
Background: In patients with post-stroke hemiplegia, coordinated reaching and grasping movements may be impaired. Patients frequently adopt compensatory strategies, which require investigation.Objectives: This study examined compensatory strategies using kinematic parameters by assessing the reach-to-grasp-pen movements in stroke patients and unaffected participants – and whether such assessment distinguished between patients with different levels of stroke, and none.Methods: Twelve stroke patients with mild impairment, 12 with moderate impairment and 10 healthy individuals performed a reach-to-grasp-pen task. Results: Stroke patients showed longer reaching movement time, less smooth movement trajectories and more trunk rotation (P ＜ 0.05). In stroke patients, the MCP and PIP of the thumb were flexed in the starting position; the MCP and PIP joints of the index finger in the stroke group were more extended during pen grasp; the range of motion of the MCP of the middle finger and of the PIP joints of the middle, ring and little fingers became greater, suggesting a larger peak aperture (P ＜ 0.05). Greater extension was observed in the index finger at the end of the grasp, suggesting inadequate flexion (P ＜ 0.05).Conclusions: The reach-to-grasp-pen task can identify patients according to stroke level and shows potential as an assessment tool.
The Novel CircSLC6A6/miR-1265/C2CD4A Axis Promotes Colorectal Cancer Growth by Suppressing P53 Signaling Pathway
Authors: Rong Zeyin , Luo Zai , Fu Zhongmao , Zhang Pengshan , Li Tengfei , Zhang Jianming , Zhu Zhonglin , Yu Zhilong , Li Qi , Qiu Zhengjun , Huang Chen 
Published: June 2021

Abstract
Background: Colorectal cancer (CRC) ranks as the third most frequently diagnosed cancer and is a leading cause of cancer-related deaths. Therefore, further researches were required to identify novel and more effective diagnoses and to identify molecular targets in treatment of CRC.Methods: CRC fresh frozen tissues and cell lines were used to detect C2CD4A expression by qRT-PCR and western blotting. The biological functions of C2CD4A were performed in vitro and in vivo. Western blotting, cDNA array, IP-MS, Co-IP, and Ubiquitination assay were used to analyze the interaction between C2CD4A and p53. Bioinformatics analysis, FISH, RNA sequencing, luciferase reporter assay, RNA immunoprecipitation, RNA pull-down and rescue experiments, were deployed to detect upstream regulation mechanism of C2CD4A.Results: C2CD4A was aberrantly upregulated in CRC tissues compared with adjacent normal colorectal tissues. C2CD4A knockdown significantly promoted cell apoptosis and with inhibited proliferation in vitro, and tumorigenicity in vivo, whereas C2CD4A overexpression had displayed an opposite effect. Moreover, circSLC6A6 was upregulated and positively associated with C2CD4A expression in CRC tissues. C2CD4A was positively regulated by circSLC6A6 via sponging miR-1265. Fundamentally, C2CD4A inhibited P53 signaling pathway through interacting with P53 and increasing its ubiquitination and degradation.Conclusion: Our results identified that circSLC6A6/miR-1265/C2CD4A axis, which was involved in CRC via the P53 signaling pathway, could be as a therapeutic target for CRC.
Investigating Phragmites Australis Response to Copper Exposure Using Physiologic，FTIR and Metabolomic Approaches
Authors: Wu Jieting , Gao Tian , Ma Fang , Zhao Lei 
Published: June 2021

Abstract
Background and aim Phragmites australis is a landscape plant with phytoremediation functions that is widely planted worldwide. However, little is known about the metabolomic background of the resistance mechanisms of Phragmites to heavy metals during its growth and development. Methods Here, we performed copper stress studies on Phragmites and monitored physiological indicators such as malondialdehyde (MDA) and electrolyte leakage (EL). In addition, FTIR was used to study chemical composition changes in the roots, stems and leaves of Phragmites seedlings under excessive copper stress. Furthermore, LC-MS technology combined with metabolomics data processing software was used to analyze the metabolic profile of samples.Result Copper contributed to the accumulation of MDA and EL. And the results of FTIR showed that the antioxidant effects of flavonoids and amino acids can be used by Phragmites leaf tissue to improve the tolerance of copper under 5 mg/L concentration. Further, the results of metabolomics reflected that Phragmites can improve its resistance to copper by increasing the accumulation of arginine and ayarin in the body. The former is accumulated through two pathways: the citrulline decomposition and conversion pathway and the circular pathway composed of ornithine, citrulline, L-Argininosuccinate and arginine. The latter is synthesized through the quercetin methylation pathway.Conclusion This study provides insights into the resistance mechanism and repair performance of Phragmites and other plant accumulators in response to copper stress.
Hsp70-containing Extracellular Vesicles are Capable of Activating of Adaptive Immunity in Models of Mouse Melanoma and Colon Carcinoma
Authors: Komarova Elena Y. , Suezov Roman V. , Nikotina Alina D. , Aksenov Nikolay D. , Garaeva Luiza А. , Shtam Tatiana A. , Zhakhov Alexander V. , Martynova Marina G. , Bystrova Olga A. , Istomina Maria S. , Ischenko Alexander M. , Margulis Boris A. , Guzhova Irina V 
Published: June 2021

Abstract
BackgroundThe release of Hsp70 chaperone from tumor cells is found to trigger the full-scale anti-cancer immune response. Such release and the proper immune reaction can be induced by the delivery of recombinant Hsp70 to a tumor and we sought to explore how the endogenous Hsp70 can be transported to extracellular space leading to the burst of anti-cancer activity. MethodsHsp70 transport mechanisms were studied by analyzing its intracellular tracks with Rab proteins as well as by using specific inhibitors of membrane domains. To study Hsp70 forms released from cells we employed the assay consisting of two affinity chromatography methods. Hsp70 content in culture medium and extracellular vesicles (EV) was measured with the aid of ELISA. The properties and composition of EVs were assessed using nanoparticle tracking analysis and immunoblotting. The activity of immune cells was studied using an assay of cytotoxic lymphocytes, and for in vivo studies we employed methods of affinity separation of lymphocyte fractions.Results Analyzing B16 melanoma cells treated with recombinant Hsp70 we found that the chaperone triggered extracellular transport of its endogenous analog in soluble and enclosed in EVs forms; both species efficiently penetrated adjacent cells and this secondary transport was corroborated with the strong increase of Natural Killer (NK) cell toxicity towards melanoma. When B16 and CT-26 colon cancer cells before their injection in animals were treated with Hsp70-enriched EVs, a powerful anti-cancer effect was observed as shown by a two-fold reduction in tumor growth rate and elevation of life span. We found that the immunomodulatory effect was due to the enhancement of the CD8-positive response and anti-tumor cytokine accumulation; supporting this there was no delay in CT-26 tumor growth when Hsp70-enriched EVs were grafted in nude mice. Importantly, pre-treatment of B16 cells with Hsp70-bearing EVs resulted in a decline of arginase-1-positive macrophages, showing no generation of tumor-associated macrophages. Conclusion In conclusion, Hsp70-containing EVs generated by specifically treated cancer cells give a full-scale and effective pattern of anti-tumor immune responses.