bluuebunny/crossref_metadata_2025_split huggingface dataset:
A gut microbial metabolite of dietary polyphenols reverses obesity-driven hepatic steatosis
Authors: Osborn Lucas J. , Schultz Karlee , Massey William , DeLucia Beckey , Choucair Ibrahim , Varadharajan Venkateshwari , Fung Kevin , Horak Anthony J. , Orabi Danny , Nemet Ina , Nagy Laura E. , Wang Zeneng , Allende Daniela S. , Sangwan Naseer , Hajjar Adeline M. , McDonald Christine , Ahern Philip P. , Hazen Stanley L. , Brown J. Mark , Claesen Jan 
Published: September 2021

AbstractThe molecular mechanisms by which dietary fruits and vegetables confer cardiometabolic benefits remain poorly understood. Historically, these beneficial properties have been attributed to the antioxidant activity of flavonoids. Here, we reveal that the host metabolic benefits associated with flavonoid consumption actually hinge on gut microbial metabolism. We show that a single gut microbial flavonoid catabolite is sufficient to reduce diet-induced cardiometabolic disease burden in mice. Dietary supplementation with elderberry extract attenuated obesity and continuous delivery of the catabolite 4-hydroxphenylacetic acid was sufficient to reverse hepatic steatosis. Analysis of human gut metagenomes revealed that under one percent contains a flavonol catabolic pathway, underscoring the rarity of this process. Our study will impact the design of dietary and probiotic interventions to complement traditional cardiometabolic treatment strategies.One-Sentence SummarySelect gut microbes can metabolize flavonoids from a fruit and vegetable diet to monophenolic acids, which improve fatty liver disease.Graphical abstract
Determination of m6A frequency utilizing 4SedTTP-RT Ligation Assisted PCR (SLAP) in viral and cellular long non-coding RNAs
Authors: Martin Sarah E , Gan Huachen , Sztuba-Solinska Joanna 
Published: September 2021

AbstractN6-methyladenosine is one of the most abundant epitranscriptomic signatures that can affect every aspect of RNA biology, from structure and stability to intra- and intermolecular interactions. The accurate quantitative assessment of RNA stoichiometry at single-nucleotide resolution is a prerequisite to evaluate the biological significance of m6A in the context of specific RNA. We have developed a new method, termed 4-Selenothymidine 5’-triphosphate reverse transcription and Ligation Assisted PCR analysis (SLAP), for quantitative and unbiased assessment of the m6A fraction on target RNA. The inclusion of thymidine triphosphate derivative during reverse transcription discourages base pair formation with m6A resulting in the reaction’s cessation, while maintaining normal A-T base pairing. The site-specific ligation of the resulting cDNAs with adapters, followed by amplification, generates two distinct products that reflect the modified and unmodified fraction of the analyzed RNA. These PCR products are subsequently separated by gel electrophoresis and quantified using densitometric analysis. We applied the SLAP to verify the position and assess the frequency of m6A sites present on two exemplary long non-coding RNAs. We assessed the SLAP specificity, accuracy, and sensitivity, proving the applicability of this method for the m6A analysis on less abundant transcripts. Overall, this method constitutes an extension of the bird’s-eye view of RNA m6A landscape provided by epitranscriptome-wide analyses by delivering quantitative assessment of modification frequency and can therefore aid the understanding of the consequences of m6A on biological processes.Graphical Abstract
ZRC3308 monoclonal antibody cocktail shows protective efficacy in Syrian hamsters against SARS-CoV-2 infection
Authors: Yadav Pragya D , Mendiratta Sanjeev Kumar , Mohandas Sreelekshmy , Singh Arun K , Abraham Priya , Shete Anita , Bandyopadhyay Sanjay , Kumar Sanjay , Parikh Aashini , Kalita Pankaj , Sharma Vibhuti , Pandya Hardik , Patel Chirag G , Patel Mihir , Soni Swagat , Giri Suresh , Jain Mukul 
Published: September 2021

AbstractWe have developed a monoclonal antibody (mAb) cocktail (ZRC-3308) comprising of ZRC3308-A7 and ZRC3308-B10 in the ratio 1:1 for COVID-19 treatment. The mAbs were designed to have reduced immune effector functions and increased circulation half-life. mAbs showed good binding affinities to non-competing epitopes on RBD of SARS-CoV-2 spike protein and were found neutralizing SARS-CoV-2 variants B.1, B.1.1.7, B.1.351, B.1.617.2 and B.1.617.2 AY.1 in vitro. The mAb cocktail demonstrated effective prophylactic and therapeutic activity against SARS-CoV-2 infection in Syrian hamsters. The antibody cocktail appears to be a promising candidate for the prophylactic use and for therapy in early COVID-19 cases which have not progressed to severe disease.
Small molecule screen employing patient-derived iPS hepatocytes identifies LRRK2 as a novel therapeutic target for Alpha1 Antitrypsin Deficiency
Authors: Kent Deniz , Ng Soon Seng , Khoshkenar Payam , Syanda Adam M. , Li Chao Zheng , Zieger Marina , Greer Cindy , Hatch Stephanie , Segal Joe , Blackford Samuel J.I. , Chowdary Vivek , Ismali Taylor , Danovi Davide , Sahdeo Sunil , Ebner Daniel , Mueller Christian , Rashid S. Tamir 
Published: September 2021

AbstractAlpha-1 antitrypsin deficiency is a life-threatening condition caused by inheritance of the SERPINA1 gene ‘Z’ variant. This single base pair mutation leads to protein misfolding, ER entrapment and gain of toxic function. Despite the significant unmet medical need presented by this disorder, there remain no approved medicines and the only curative option is liver transplantation. We hypothesized that an unbiased screen of human hepatocytes harbouring the Z mutation (ATZ) using small molecules targeted against protein degradation pathways would uncover novel biological insights of therapeutic relevance. Here we report the results of that screen performed in a patient-derived iPSC model of ATZ. Starting from 1,041 compounds we identified 14 targets capable of reducing polymer burden, including Leucine-rich repeat kinase-2 (LRRK2), a well-studied target in Parkinson’s. Genetic deletion of LRRK2 in ATZ mice reduced polymers and associated fibrotic liver disease leading us to test a library of commercially available LRRK2 kinase inhibitors in both patient iPSC and CHO cell models. One of the molecules tested, CZC-25146, reduced polymer load, increased normal AAT secretion and reduced inflammatory cytokines with pharmacokinetic properties supporting its potential use for treating liver diseases. We therefore tested CZC-25146 in the ATZ mouse model and confirmed its efficacy for polymer reduction without signs of toxicity. Mechanistically, in both human and mouse models, our data show CZC-25146 inhibits LRRK2 kinase activity and induces autophagy. Cumulatively, these findings support the use of CZC-25146 and LRRK2 inhibitors in general in hepatic proteopathy disease research and as potential new treatment approaches for patients.One Sentence SummaryA small molecule screen in patient iPSCs with in vivo validation in mice identifies LRRK2 as a new therapeutic target for Alpha-1 Antitrypsin Deficiency.
Empowering human resource functions with data-driven decision-making in start-ups: a narrative inquiry approach
Authors: Varma Deepkumar , Dutta Pankaj 
Published: September 2021

Purpose
Across industries, firms want to adopt data-driven decision-making (DDDM) in various organizational functions. Although DDDM is not a new paradigm, little is known about how to effectively implement DDDM and which problem areas to focus on in these functions. This study aims to enable start-ups to use DDDM in human resources (HR) by studying five HR domains using a narrative inquiry technique and aims to guide managers and HR practitioners in start-ups to enable data-driven decisions in HR.

Design/methodology/approach
This study adopts the narrative inquiry technique by conducting semi-structured interviews with HR practitioners and senior members handling HR functions in start-ups. Interview memos are thematically analyzed to identify repeated ideas, concepts or elements that become apparent.

Findings
The study findings indicate that start-ups need to have canned operational reports with right attributes in each of these HR domains, which members should use when performing HR tasks. Few metrics, like cost-to-hire in recruitment, distinctly surfaced relatively higher in importance that each start-up, should compute and use in decision-making.

Practical implications
Managers, HR practitioners and information technology implementation teams will be able to consume the findings to effectively design or evaluate HR processes or systems that empower decision-making in a start-up.

Originality/value
Start-ups have a fast-paced culture where creativity, relationships and nimbleness are valued. Prevalent decision models of larger organizations are not suitable in start-ups’ environments. This study, being cognizant of these nuances, takes a fresh approach to guide start-ups adopt DDDM in HR and identify key problem areas where decision-making should be enabled through data.
Impact of environmental management practices on corporate sustainability: evidence from the Maldives hospitality industry
Authors: Moosa Anitha , He Feng 
Published: September 2021

PurposeThis paper aims to explore how environmental management practices impact different dimensions of corporate sustainability. It also explores the mediating impact of environmental regulation and reports on the relationship between environmental management practice and corporate sustainability.Design/methodology/approachA brief focus group discussion and a preliminary test were conducted through a focused group meeting with industry experts before data were collected from senior management of 116 registered operations in the hospitality and tourism industry in the Maldives. To analyse the data, a mediation model is proposed and tested using partial least squares structural equation modelling.FindingsResults showed that environmental management practices have a direct and positive effect on corporate sustainability. Furthermore, environmental regulation and reporting positively mediate the effect of environmental management practices on corporate sustainability. Among the sustainability dimensions, it is important to note that the social sustainability aspect has the highest impact, followed by the economic and environmental aspects of corporate sustainability.Practical implicationsFindings provide empirical evidence in understanding achieving corporate sustainability through environmental management practices. The study is practical for stakeholders and policymakers to follow through with the environmental regulations and be transparent on environmental reporting measures that impact overall sustainability.Originality/valueThis study serves as noteworthy research for stakeholders to evaluate against regulatory and reporting requirements for businesses they invest in the future. It adds value to the literature and attempts to advance environmental management and sustainability research in the context of small island developing states.
The effect of self-fulfilling prophecy on developing auditors' ethical values
Authors: Rezaeirad Mostafa , Koushki Jahromi Alireza 
Published: September 2021

Purpose
The growth and development of positive psychology approaches in the areas of human resource management has created a successful conceptual basis in the psychology of business and jobs and has led to a tendency to apply knowledge and skills to job expectations in line with job expectations. That profession will grow, and this can also lead to the development of ethical practices. The purpose of this paper is the effect of self-fulfilling prophecy on developing auditors' ethical values.

Design/methodology/approach
The target population of this study was auditors of auditing organizations and private sector audit firms that were selected through random sampling and evaluated over a period of 6 months. The research instrument was standard questionnaires, and partial least squares analysis was used to test and test the research hypotheses.

Findings
The results of this study show that the effect of self-fulfilling prophecy on ethical virtue and ethical conscientiousness as two dimensions of auditors' ethical values has a positive and significant effect.

Originality/value
This study explains how to clearly convey the social expectations of an auditor about value-based approaches in the audit profession, and it examines the role of these expectations in the professional performance of auditors. In fact, beliefs and expectations play a decisive role in improving auditors' level of value based on professional behavior, such as professional skepticism and objectivity, and this research can help increase the level of knowledge about this profession.
The effect of the number of bank relationships on firm leverage: high-risk shift and the curse of mainstream banks
Authors: Dinc Yusuf , Bilgin Rumeysa 
Published: September 2021

Purpose
Firms prefer to have more than one bank relationship to secure the flow of funds for their operations, particularly in bank-based economies. On the other hand, banks lean toward expanding their customer base with firms already in the credit market. The purpose of this study is to investigate the effect of the number of bank relationships as a firm-specific determinant of capital structure and to discuss its impact on the banking sector.

Design/methodology/approach
A two-step system generalized method of the moments estimation method is used in this study. The sample comprises 213 Turkish non-financial, publicly listed firms with a positive shareholder’s value for the 2012–2017 period.

Findings
The findings show that the number of bank relationships increases the leverage of sample firms while the concentration in the banking sector decreases it. These rather intriguing findings are attributed to an under-the-counter credit policy that causes a high-risk shift and a curse of mainstream banks. Once the mainstream banks allocated credit to the firm, its credibility is consumed by the following banks, which is implied by the significantly negative relationship between bank concentration and firm leverage. This problem is defined as the mainstream bank curse in the study.

Originality/value
The previous literature focuses on the effects of the number of bank relationships on firm profitability, cost of debt and shareholder wealth. However, its impact on the capital structure has not yet been systematically investigated. To the authors’ knowledge, this is the first study to critically analyze the effect of the number of bank relationships on the capital structure. The findings will be of immense benefit to the banking sector and the regulatory bodies.
Voluntary disclosure and complexity of reporting in Egypt: the roles of profitability and earnings management
Authors: Alm El-Din Mai Mohammed , El-Awam Atef Mohammed , Ibrahim Farid Moharram , Hassanein Ahmed 
Published: September 2021

PurposeThe study explores the relationship between information overloading and the complexity of reporting. In particular, it investigates whether voluntary information in a firm annual report is associated with its readability. Likewise, it examines how a firm's profitability and earnings management practices impact the nexus of voluntary disclosure and readability.Design/methodology/approachIt uses the annual reports of the Egyptian nonfinancial firms listed in the EGX 100 index from 2010 to 2018. The readability of the annual report is measured automatically using the LIX index, and a predeveloped voluntary disclosure index is used to measure the level of voluntary disclosure in the annual reports.FindingsThe results reveal that the readability of annual reports is a negative function of voluntary disclosure, suggesting that Egyptian firms with more voluntary disclosure are likely to have more complex (i.e. less readable) annual reports. Likewise, less profitable firms and firms with earning management practices increase voluntary information in their annual reports, resulting in an adverse impact on their reporting readability.Research limitations/implicationsIt focuses only on the annual reports of Egyptian firms and considers a firm’s overall voluntary information rather than a particular area of voluntary disclosure. It introduces a code to measure the readability of Arabic-written texts, which can be applied to different areas of disclosure.Practical implicationsPolicymakers in Egypt are encouraged to develop enforceable regulations to control voluntary disclosure in annual reports. Egyptian investors should view the practice of higher voluntary disclosure skeptically as its aim may be to divert attention from a firm's poor performance and earnings management practice.Originality/valueThe study is the first evidence from Egypt on the effect of information overloading, proxied by voluntary disclosure, on the readability of reporting. Likewise, it contributes to methodological development in measuring the readability of Arabic-written annual reports.
Capture coproduction behavior in networking alumni communities: Progress from platform belongingness, knowledge sharing, and citizenship behavior
Authors: Bui My-Trinh , Jeng Don Jyh-Fu 
Published: September 2021

Purpose
The purpose of this study is to investigate coproduction behavior in networking alumni communities via the progress from platform belongingness, knowledge sharing and citizenship behavior. Alumni networking communities have emerged as valuable assets for conserving institutional resources, supporting members and contributing new resources for alumni-institutional professional development. However, the previous literature has not yet captured the explicit processes by which these contributions are made.

Design/methodology/approach
Data from 711 respondents selected from an alumni collaboration network were subjected to structural equation modeling analysis.

Findings
The study explored resource conservation (belongingness) as the primary relational mechanism for alumni to share their instrumental resources (knowledge sharing), supporting resources (citizenship behavior) and competent resources (coproduction behavior). Knowledge sharing and citizenship behavior act as intermediate agents to trigger coproduction behavior. The authors show how subjective norm, group norm and trust is regarded as a tool to reduce bonding intrusiveness (i.e. the intrusive side-effects of a bond) and moderate the indirect effect of belongingness on coproduction and the direct effect of citizenship on coproduction.

Research limitations/implications
By applying attachment theory, conservation of resources theory and digital platform networking perspectives, this study describes major implications for designing inspiring and compatible community platforms.

Practical implications
Guidance is provided for improving sustainable alumni communities through citizenship-sharing and coproduction behavior.

Social implications
Online alumni communities are regarded as resource conservators, which can result in valuable coproduction, via the sharing of knowledge, expertise and skillsets to create profit for a range of institutions and industries.

Originality/value
Alumni networking platforms encourage alumni cohesiveness, stimulate knowledge exchange and improve professionalism.
Economic study of maintenances and outage resolution in an electricity distribution network using the continuous-time Markov chain
Authors: Nazhand Navid , Dashti Reza , Ahmadi Abolfazl 
Published: September 2021

PurposeThe purpose of this paper is to describe a novel method to compromise between planned (regulated) maintenance and outage initiation and unplanned (unregulated) maintenance and to find an economic model using which one can perform maintenance adequately and in the most optimal state.Design/methodology/approachIn this paper, a system consisting of similar components is considered, and the role of each component in the system is explained. Then, the cost pertaining to failure in each asset is determined. Costs such as energy not supplied, penalties, human resources to resolve the defect and replacing assets are taken into account. Finally, a new comprehensive objective is proposed, and optimization is performed for a sample system.FindingsIn this paper, some graphs have been plotted from which plenty of information may be extracted. This is mentioned in the Conclusion.Originality/valueIn this paper, some graphs have been plotted from which plenty of information may be extracted. This is mentioned in the Conclusion.
Reinterpreting excellence for sustainable competitive advantage: the role of entrepreneurial culture under information technological turbulence
Authors: Pratono Aluisius Hery 
Published: September 2021

Purpose
This study aims to understand the culture of excellence by examining the role of entrepreneurial culture in shaping how firms achieve sustainable competitive advantage (CA). This study takes into consideration the firms’ capability to transform the entrepreneurial culture into a sustainable CA by generating product development and adapting the information technological turbulence.

Design/methodology/approach
This study first gathers evidence from literature then carries out a detailed study to propose a structural equation model followed by an online survey that supports empirical evidence. This empirical test involves a data set with 782 usable responses following the 4,000 emails sent to the respondents and removed data due to the missing values. The population data are taken from the firm directory in Surabaya City that the Indonesian Ministry of Trade and Industry published.

Findings
There is a strong tendency that entrepreneurial culture is imperative for firms to attain sustainable CA by supporting new product development. The results show that product development provides a partial mediating effect, which indicates that entrepreneurial culture may affect the sustainable CA directly and with the product development support. This study also touches on dynamic capability by proposing a scenario approach that suggests that firms should refine the entrepreneurial culture to adapt to the information technological turbulence.

Originality/value
This study extends the understanding of the culture of excellence by underpinning the dynamic capability theory, which argues that entrepreneurial culture is a valuable resource, which helps firms achieve sustainable CA by promoting product development.
Repeatability of migratory behaviour suggests trade‐off between size and survival in a wild iteroparous salmonid
Authors: Birnie‐Gauvin Kim , Koed Anders , Aarestrup Kim 
Published: September 2021

AbstractRepeatable individual variation in behaviour has been demonstrated in all taxa, though few studies have explored the broader ecological consequences of such consistent behaviour.We tagged and tracked 401 individual sea troutSalmo truttawith passive integrated transponder telemetry over two to four spawning seasons as they migrated between freshwater and marine environments to spawn and feed respectively.We found the timing (time of river entry) and duration of migration (time spent in freshwater and time spent at sea) to be repeatable within individuals across years. In addition, we identified two peaks in return migration timing; a group of summer‐migrating fish and a group of fall‐migrating fish. At the time of first spawning, sea trout differed in size depending on how long the first marine phase was, but summer‐migrating fish were smaller in all subsequent spawnings. Furthermore, summer‐migrating fish had significantly better marine survival than fall‐migrating fish.Our study suggests that the timing of arrival in freshwater is an important fitness‐relevant trait, such that summer‐migrating fish have decreased fitness but remain in somewhat safer conditions, while fall‐migrating fish have increased fitness but are exposed to more risky conditions. Taken together, our findings suggest that migration timing and duration is determined via a trade‐off between size and survival, which resulted in two evolutionary stable strategies.A freePlain Language Summarycan be found within the Supporting Information of this article.