bluuebunny/crossref_metadata_2025_split huggingface dataset:
Deep brain stimulation versus nonsurgical treatment for severe Alzheimer's disease: A long-term retrospective cohort study
Authors: Xu Junpeng , Liu Bin , Feng Zhebin , Yu Xinguang , Shang Guosong , Liu Yang , Sun Yuxiang , Yang Haonan , Chen Yuhan , Zhang Yanyang , Mao Zhiqi 
Published: December 2024

Background Severe Alzheimer's disease (AD) is characterized by significant neuropsychiatric symptoms and sleep disorders, with limited effectiveness of conservative drug treatments. Deep brain stimulation (DBS) offers a potential alternative. Objective To evaluate the efficacy, safety, and long-term outcomes of DBS versus conservative treatment in patients with severe AD. Methods We retrospectively analyzed 40 patients with severe AD diagnosed at the People's Liberation Army General Hospital from 2015 to 2022. Twenty patients received DBS, and twenty received conservative treatment. Treatment effects were assessed using standardized scales at three- and twelve-months post-treatment. Primary outcomes included changes in cognitive function [Mini-Mental State Examination (MMSE), Montreal Cognitive Assessment (MoCA), Alzheimer's Disease Rating Scale-Cognitive subscale, Clinical Dementia Rating). Secondary outcomes included quality of life, sleep quality, neuropsychiatric symptoms, and caregiver burden (Barthel Index, Functional Activity Questionnaire, Functional Independence Measure (FIM), Neuropsychiatric Inventory (NPI), Hamilton Anxiety Rating Scale (HAM-A), Hamilton Depression Rating Scale (HAM-D), Pittsburgh Sleep Quality Index (PDQI), Zarit Burden Interview (ZBI)]. Results DBS patients showed significantly greater improvements in MMSE, MoCA, FIM, and ZBI scores than controls, suggesting improved cognitive function and quality of life, and reduced caregiver burden (p < 0.05). Notably, DBS significantly reduced HAM-A, HAM-D, and PSQI scores, and improved NPI scores more than controls, indicating significant amelioration of neuropsychiatric symptoms and sleep disorders (p < 0.05). Conclusions DBS is a safe and reversible treatment that potentially enhances cognitive function and quality of life in severe AD patients and alleviates caregiver burden. For the first time, we report that DBS also improves neuropsychiatric symptoms and sleep disorders, highlighting its clinical potential in AD.
Synergistic activity of nootropic herbs as potent therapeutics for Alzheimer's disease: A cheminformatics, pharmacokinetics, and system pharmacology approach
Authors: Don Bosco Reiya Bosco , Selvan Christyraj Johnson Retnaraj Samuel , Yesudhason Beryl Vedha 
Published: December 2024

Background Alzheimer's disease (AD) is a progressive neurodegenerative disorder, which subdues over 55 million people and finding a cure, still remains disenchanting. Indian medicinal herbs notably, Withania somnifera, Bacopa monnieri, Curcuma longa, and Clitoria ternatea are traditionally utilized for their memory-enhancing properties. Objective We computationally investigated the therapeutic potential of four nootropic herbs by uncovering the molecular mechanisms underlying their treatment for AD. Methods Cheminformatics, pharmacokinetics, and system pharmacology studies were carried out to predict the phytocompounds drug-like properties, protein targets, targets functional association and enrichment analysis. A comparative study was performed with phytocompounds and FDA-approved drugs. Investigation on the expression of protein targets in the hippocampus and entorhinal cortex of the AD brain was performed. Network was constructed to depict the interaction between phytocompounds, drugs, and molecular targets. Results Through comparative analysis, we found that the phytocompounds shared common targets with both FDA drugs and drugs under clinical trials. We identified potential active compounds notably, Withaferin A, Withanolide-D, Withanolide-E, Withanolide-G, and Humulene epoxide II, that can combat AD. Interestingly, the enzyme inhibition scores of the identified drugs were much higher than FDA-approved drugs. In addition, regulatory proteins such as AβPP, acetylcholinesterase, BACE1, and PTPN1 were the targets of 8, 16, 9, and 22 phytocompounds, respectively. Nonetheless, AR and CYP19A, were the primary targets of most phytocompounds. Conclusions Herbal medicines can synergistically stimulate multiple protein targets, rendering a holistic and integrative treatment, encouraging a promising avenue to treat AD.
Gut microbiome synthesizes important core metabolites to prevent cognitive decline and mitigate onset and progression of Alzheimer's disease
Authors: Al-Abbas Nouf S , Shaer Nehad A 
Published: December 2024

Background This study explores how gut metabolites, produced through bacterial metabolism in the gut, influence neurological conditions like Alzheimer's disease (AD). Key metabolites such as succinate and short-chain fatty acids signal through the autonomic nervous system and can cross the blood-brain barrier, impacting central nervous system functions. Objective The aim is to examine the role of the gut microbiota in compensating for metabolic deficiencies in AD. By analyzing wild-type (WT) and APP/PS1 mice, the study investigates how the microbiome affects key metabolic processes and whether it can slow AD progression. Methods High-throughput sequencing data from the gut microbiomes of APP/PS1 transgenic AD model mice and age-matched WT C57BL/6 male mice were analyzed for microbial and metabolite profiles. Results Alpha and beta diversity analyses showed differences in microbial composition between groups. Partial least squares discriminant analysis and Anosim confirmed distinct microbiome profiles in WT and APP/PS1 mice. At the genus level, Vescimonas was more abundant in WT mice, while Odoribacter, Lacrimispora, Helicobacter, Bacteroides, and Alloprevotella were more prevalent in APP/PS1 mice. Conclusions While taxonomic differences did not directly link specific microorganisms to AD, functional analysis identified key metabolites—acetyl-CoA, glucose, succinate, lipids, choline, and acetylcholine—that may alleviate energy deficits and synaptic dysfunction. This study suggests that the microbiome may help compensate for AD-related impairments, opening avenues for microbiome-based therapies.
“Struggling for Years”: An international survey on Autistic experiences of menopause
Authors: Jenkins Christine A. , Moseley Rachel L. , Matthews Rose J. , van Rensburg Margaret Janse , Gamble-Turner Julie M. , Brady Miranda J. 
Published: December 2024

Emerging research suggests that menopause can be a difficult transition for some Autistic people. This international co-produced survey explored factors that might make menopause harder for Autistic people, such as lack of menopause and autism awareness; moreover, we aimed to explore what support Autistic people needed and what could help them cope and even flourish during this time. We employed a mixed-methods approach to analyze quantitative and qualitative responses from 508 Autistic participants. Both the quantitative and qualitative data supported relationships between lack of menopause and autism awareness and overall difficulties during menopause. Autistic participants wanted more appropriate and Autistic-specific information about menopause, and perceived lack of clinician awareness about both menopause and autism as barriers to care and support. Finally, participants reported discovery of ways to cope and even flourish during menopause through self-awareness and self-acceptance. Better awareness of autism and access to Autistic-specific menopause information is important in helping Autistic people transition through menopause. Lay abstract We believe that many Autistic people may struggle with menopause. However, we know very little about factors that might make menopause more difficult for Autistic people, and things that might help them through this transition. Our online survey, co-produced with Autistic people, explored whether a lack of awareness about menopause and autism might lead to a more difficult menopause experience. We also aimed to understand what kind of support and information Autistic people need in menopause, and what, if anything, helped Autistic people in menopause cope and even flourish through this life transition. We received 508 usable responses from Autistic participants in 24 different countries. Overall, the study found a relationship between lack of menopause awareness and difficulty of menopause: i.e., that those who had less knowledge about menopause tended to have a harder time. The study also found that lack of awareness of one’s autism at the start of menopause was associated with a more difficult menopause experience. Participants reported that they wished they had had more appropriate and autistic-specific information about menopause before it started. They also indicated that a lack of information and training amongst clinicians about both menopause and autism were barriers to support. Finally, greater self-awareness and self-acceptance amongst other coping mechanisms helped participants. Our findings highlight where there are gaps in knowledge and support for Autistic people during menopause. They highlight that knowledge about menopause, and about one’s autism, are extremely important. They also highlight coping strategies that some Autistic people have found helpful.
Differential dissolving kinetics of trioctahedral lizardite, chlorite and talc, and water-interface reactions in an acid environment
Authors: Zhao Dingran , Sun Hongjuan , Peng Tongjiang , Zeng Li 
Published: December 2024

Abstract
Different two-dimensional structural units of layered silicate minerals have different chemical and reaction properties. Sulfuric acid solution mineral-leaching systems with pH of 2.0, 4.0 and 6.0 were constructed to investigate the differential dissolution properties of lizardite (1:1 type), chlorite and talc (2:1 type minerals) and the chemical kinetic mechanism of the mineral–water interface reaction. The results showed that the dissolution efficiency of Mg in lizardite is higher than that of chlorite and talc in acidic environments (pH of 2.0, 4.0 and 6.0). The dissolution efficiency of Mg in chlorite is greater than that of talc for acidic environments when pH is 2.0 and 4.0, but chlorite and talc have nearly identical Mg dissolution efficiencies at a pH of 6.0. This phenomenon is related to the defect site on the tetrahedral sheet of chlorite and is controlled by the change of the dissolution efficiency of Al. The dissolution rates of Mg and Si in lizardite, chlorite and talc decreased with the increase of reaction time in the acidic medium for pH = 2.0, 4.0 and 6.0, and there are two linear dissolution trends at different pH values. The dissolution efficiencies of Mg and Si in lizardite, chlorite and talc were simulated and predicted by establishing a logistic model. It was found that the maximum dissolution efficiency of 2:1 type minerals chlorite and talc are only 4.72% and 1.58%, which is much lower than that of 1:1 type lizardite. This research on the reaction mechanism and dissolution kinetics of lizardite, chlorite and talc not only helps to deepen the understanding of the mineral–water interface interaction, but also reveals the different rules for Mg, Si and Al dissolution in different types of trioctahedral mineral–water interface reactions, and provides a crystal chemical basis for the ion migration and action mechanism of minerals.
RBM15-dependent m6A modification mediates progression of non-small cell lung cancer cells
Authors: Wang Man , Qin Yujiao , Ai Xiaoqi , Liu Xiuhua 
Published: December 2024

Abstract
Background
Non-small cell lung cancer (NSCLC) is the predominant form of lung cancer, contributing significantly to global health and economic challenges. This study elucidated the role of RBM15 in NSCLC progression through its involvement in m6A modifications.

Methods
RBM15 levels in NSCLC tissues and cells were assessed via RT-qPCR and Western blotting. The impact of RBM15 knockdown on NSCLC proliferation, invasion, and migration was evaluated using CCK-8, colony formation, and Transwell assays. Expression levels of KLF1, TRIM13, and ANXA8 were determined by RT-qPCR and Western blot. m6A methylation levels were analyzed, while RIP and MeRIP assays were employed to explore the interaction between YTHDF1/YTHDF2/m6A and KLF1/TRIM13, as well as KLF1 binding to the ANXA8 promoter. The ubiquitination of ANXA8 was examined through ubiquitination assays. Xenograft and metastasis models were utilized to assess RBM15’s role in vivo.

Results
RBM15 was found to be overexpressed in NSCLC. Silencing RBM15 led to decreased cell proliferation, invasion, and migration of NSCLC cells. RBM15 upregulated KLF1 and downregulated TRIM13 via YTHDF1/YTHDF2, resulting in the promotion of ANXA8 expression. KLF1 overexpression or TRIM13 downregulation partially reversed the suppressive effects of RBM15 knockdown on NSCLC cell proliferation. ANXA8, upregulated in NSCLC, mitigated the inhibitory effects of RBM15 silencing on malignant behaviors. In vivo, RBM15 downregulation hindered NSCLC cell proliferation and metastasis by modulating the KLF1-TRIM13/ANXA8 axis.

Conclusion
RBM15-mediated m6A methylation enhances KLF1 expression and suppresses TRIM13 via YTHDF1/YTHDF2, thereby promoting ANXA8 and facilitating NSCLC progression. These findings provide novel insights and potential therapeutic targets for NSCLC treatment.
Digital Twin-Based Production-Logistics Synchronization System for Satellite Mass Assembly Shop-Floor
Authors: Liu Weiran , Zou Xiaofu , Wen Zhiwen , Cheng Jiangfeng , Zuo Ying , Zhang Chenyuan , Liu Hongting , Tao Fei 
Published: December 2024

AbstractIn recent years, the rapid development of large-scale satellite constellations has challenged the mass production capabilities of satellite manufacturers. Assembly is the last and critical phase of satellite production. Achieving satellite mass assembly is the key to realizing satellite mass production. To this end, satellite manufacturers are working to construct the satellite mass assembly shop-floor (SMAS) to enable moving assembly. However, there is still a lack of a modularized manufacturing system oriented to flexible production for SMAS, as well as disturbance detection methods and production-logistics synchronization methods to deal with various disturbances. Therefore, this paper proposes a digital twin-based production-logistics synchronization system (DT-PLSS) for SMAS. The framework of DT-PLSS is introduced first. In this framework, DT-PLSS can achieve modular construction, as well as distributed management and control. Based on the proposed framework, the construction methods of resource level digital twin (DT), workstation level DT, and shop-floor level DT in SMAS are discussed. The DT-based disturbance detection method for SMAS is presented, aiming to detect or predict different types of disturbances and to analyze the effect of disturbances. Then, a DT enhanced production-logistics synchronization mechanism for SMAS is proposed. With this mechanism, the logistics distribution in the dynamic shop-floor environment and production-logistics synchronization under various disturbances can be realized. Finally, a case study in a real SMAS verifies the feasibility and effectiveness of the proposed system and methods. This research proposes a practical framework and system which could realize disturbance detection, logistics distribution, and the production-logistics synchronization in complex SMAS scenario effectively.
Indication and functional outcome of orthotopic neo-bladder reconstruction following radical cystectomy for bladder cancers in a specialist hospital in north-west Cameroon
Authors: Ngwa-Ebogo Tagang Titus , Nchomboh Nwenasi McRyan , Mbouche Landry Oriole , Tamufor Njuma Emmanuel , Forcha Tandu Yannick , Mbassi Achille Aurore , Fouda Pierre Joseph , Ashuntantang Gloria Enow , Angwafor III Fru Forbuzshi 
Published: December 2024

Abstract
Background
Radical cystectomy (RC) and urinary diversion are treatment modalities for muscle invasive bladder cancer (MIBC). However, in Cameroon, there is little information on orthotopic neo-bladder (ONB) reconstruction in the literature.

Objective
We aimed to share our indications for ONB reconstruction, evaluate the functional outcomes, and enumerate the surgical complications. By sharing our experience with ONB reconstruction, we hope to broaden the understanding of this procedure and aid in anticipating its various outcomes.

Methods
A retrospective assessment of medical records was conducted for all patients who underwent ONB reconstruction after RC for bladder cancer from January 2020 to April 2023 at Nkwen Baptist Hospital. Data collected included socio-demographic details, clinical and pathological data, indications for surgery, voiding, and metabolic outcomes at 6 and 12 months postoperatively. Data analysis was performed using IBM-SPSS version 26.0.

Results
Eighteen patients (66.7% male) with a mean age of 60.50 (± 6.90) years were included. Indications for ONB were 56% for pT2b, 23% for pT2a, and 23% for multifocal T1 disease without ureteral, urethral, or bladder neck extension. At 12 months, 77.8% had acceptable diurnal continence, and 72.2% had acceptable nocturnal continence. Peak urinary flow rates and post-void residual volumes were acceptable in 77.8% and 83.3% of patients, respectively. Additionally, 88.9% had normal sodium levels, 94.4% had normal potassium levels, and 94.4% had normal chloride levels at 12 months post-ONB creation.

Conclusions
ONB reconstruction provides satisfactory continence and minimal rates of electrolyte derangement, contributing valuable insights into the functional outcomes of this procedure.
DEMETER DNA demethylase reshapes the global DNA methylation landscape and controls cell identity transition during plant regeneration
Authors: Lee Seunga , Bae Soon Hyung , Jeon Yunji , Seo Pil Joon , Choi Yeonhee 
Published: December 2024

Abstract
Background
Plants possess a high potential for somatic cell reprogramming, enabling the transition from differentiated tissue to pluripotent callus, followed by the formation of de novo shoots during plant regeneration. Despite extensive studies on the molecular network and key genetic factors involved in this process, the underlying epigenetic landscape remains incompletely understood.

Results
Here, we explored the dynamics of the methylome and transcriptome during the two-step plant regeneration process. During the leaf-to-callus transition in Arabidopsis Ler, CG methylation shifted across genic regions, exhibiting a similar number of differentially methylated regions (DMRs) for both hypo- and hypermethylation. Pericentromeric regions underwent substantial CG and extensive CHH hypomethylation, alongside some CHG hypermethylation. Upon shoot regeneration from callus, genic regions displayed extensive reconfiguration of CG methylation, while pericentromeric methylation levels highly increased across all cytosine contexts, coinciding with the activation of the RNA-directed DNA methylation (RdDM) pathway. However, mutation in DEMETER (DME) DNA demethylase gene resulted in significant genic CG redistribution and global non-CG hypomethylation in pericentromeric regions, particularly during shoot regeneration. This non-CG hypomethylation observed in dme-2 mutants was, at least partly, due to RdDM downregulation. The dme-2 mutants affected gene expression involved in pluripotency and shoot meristem development, resulting in enhanced shoot regeneration through a reprogrammed state established by pericentromeric hypomethylation compared to wild type.

Conclusion
Our study demonstrates epigenetic changes, accompanied by transcriptome alterations, during pluripotency acquisition (leaf-to-callus) and regeneration (callus-to-de novo shoot). Additionally, it highlights the functions of the DME demethylase, particularly its close association with the RdDM pathway, which underlies pericentromeric non-CG methylation maintenance. These results provide important insights into the epigenetic reconfiguration associated with cell identity transition during somatic cell reprogramming.
Beyond autonomy: unpacking self-regulated and self-directed learning through the lens of learner agency- a scoping review
Authors: Gupta Nidhi , Ali Kamran , Jiang Dan , Fink Trine , Du Xiangyun 
Published: December 2024

Abstract
Background
Learner agency involves students actively engaging in their learning process and shaping their educational experiences through autonomy, self-regulation, and decision-making. In professional education, particularly within health professions, learner agency is critical for fostering adaptability and lifelong learning. This scoping review explores how learner agency, alongside concepts such as self-regulated learning and self-directed learning, is addressed in undergraduate dental education, aiming to understand its implications and strategies for enhancing student agency in this context.

Methods
The scoping review examined literature on self-regulated learning, self-directed learning and learner agency in undergraduate dental education from 1994-April 2024 across five databases: PubMed, Scopus, Embase, ProQuest Central, and Web of Science. A manual search of the cited references was also conducted. Relevant studies were screened, and the findings were summarized to offer a comprehensive overview and identify research gaps.

Results
In total, 33 studies were included in the review. The results revealed a strong interconnection between intrapersonal, behavioral, and contextual dimensions in shaping learner agency through self-regulated learning and self-directed learning among undergraduate dental students. The studies analyzed, predominantly quantitative, highlighted the multifaceted relationships among self-regulated learning and self-directed learning and learner agency, emphasizing its significance for educational practice and policy.

Conclusions
Self-regulated learning and self-directed learning are crucial for developing learner agency, aiding undergraduate students’ transition into independent professionals and fostering lifelong learning behaviors. Educational strategies should prioritize empowering students to become independent learners, reducing their reliance on faculty. Further research is needed to identify effective methods for promoting learner agency development among dental students.