bluuebunny/crossref_metadata_2025_split huggingface dataset:
Transcriptomic analysis of resistance and short-term induction response to pyrethroids, in Anopheles coluzzii legs
Authors: Kefi Mary , Charamis Jason , Balabanidou Vasileia , Ioannidis Panagiotis , Ranson Hilary , Ingham Victoria A , Vontas John 
Published: September 2021

Abstract
BackgroundInsecticide-treated bed nets and indoor residual spraying comprise the major control measures against Anopheles gambiae sl, the dominant vector in sub-Saharan Africa. The primary site of contact with insecticide is through the mosquitoes’ legs, which represents the first barrier insecticides have to bypass to reach their neuronal targets. Proteomic changes and leg cuticle modifications have been associated with insecticide resistance that may reduce the rate of penetration of insecticides. Here, we performed a multiple transcriptomic analyses focusing on An. coluzzii legs. ResultsFirstly, leg-specific enrichment analysis identified 359 genes including the pyrethroid-binder SAP2 and 2 other chemosensory proteins, along with 4 ABCG transporters previously shown to be leg enriched. Enrichment gene families included those involved in detecting chemical stimuli including gustatory and ionotropic receptors and genes implicated in hydrocarbon-synthesis. Subsequently, we compared transcript expression in the legs of a highly resistant strain (VK7-HR) to both a strain with very similar genetic background which has reverted to susceptibility after several generations without insecticide pressure (VK7-LR) and a lab susceptible population. 232 differentially expressed genes (73 up-regulated and 159 down-regulated) were identified in the resistant strain when compared to the two susceptible counterparts, indicating an over-expression of phase I detoxification enzymes and cuticular proteins, with decrease in hormone-related metabolic processes in legs from the insecticide resistant population. Finally, we analysed the short-term effect of pyrethroid exposure on An. coluzzii legs, comparing 1hour-deltamethrin-exposed (VK7-IN) to unexposed (VK7-HR) leg transcriptomes and identified 348 up-regulated genes including those encoding for GPCRs, ABC transporters, odorant-binding proteins and members of the divergent salivary gland protein family. ConclusionsThe data on An. coluzzii leg-specific transcriptome provides valuable insights into the first line of defense in pyrethroid resistant and short-term deltamethrin-exposed mosquitoes. Our results suggest that xenobiotic detoxification is likely occurring in legs, while the enrichment of sensory proteins, ABCG transporters and cuticular genes is also evident. Constitutive resistance is primarily associated with elevated levels of detoxification and cuticular genes, while short-term insecticide-induced tolerance is linked with overexpression of transporters, GPCRs and GPCR-related genes, sensory/binding and salivary gland proteins.
m6A Demethylase FTO Promotes Tumor Progression via Regulation of Lipid Metabolism in Esophageal Cancer
Authors: Duan Xiaoran , Yang Li , Wang Liuya , Liu Qinghua , Zhang Kai , liu Shasha , Liu Chaojun , Gao Qun , Li Lifeng , Qin Guohui , Zhang Yi 
Published: September 2021

Abstract
BackgroundEpitranscriptomics studies have contributed greatly to the development of research on human cancers. In recent years, N6-methyladenosine (m6A), an RNA modification on the N-6 position of adenosine, has been found to play a potential role in epigenetic regulation. Therefore, we aimed to evaluate the regulation of cancer progression properties by m6A. ResultsWe found that m6A demethylase fat mass and obesity-associated protein (FTO) was highly expressed in esophageal cancer (EC) stem-like cells, and that its level was also substantially increased in EC tissues, which was closely correlated with a poor prognosis in EC patients. FTO knockdown significantly inhibited the proliferation, invasion, stemness, and tumorigenicity of EC cells, whereas FTO overexpression promoted these characteristics. Furthermore, integrated transcriptome and meRIP-seq analyses revealed that HSD17B11 may be a target gene regulated by FTO. Moreover, FTO promoted the formation of lipid droplets in EC cells by enhancing HSD17B11 expression. Furthermore, depleting YTHDF1 increased the protein level of HSD17B11. ConclusionsThese data indicate that FTO may rely on the reading protein YTHDF1 to affect the translation pathway of the HSD17B11 gene to regulate the formation of lipid droplets in EC cells, thereby promoting the development of EC. The understanding of the role of epitranscriptomics in the development of EC will lay a theoretical foundation for seeking new anticancer therapies.
Clonal Hematopoiesis in Lung Cancer and Its Impact on Liquid Biopsy Based on Targeted Deep Sequencing
Authors: Xue Yang , Wang Youyu , Li Yingmei , Liu Danni , Shao Mengqi , Liu Lei , Liao Yida , Huang Tanxiao , Zhang Jing , Mu Tianhao , Chen Shifu , Feng Gang , Jia Kegang 
Published: September 2021

Abstract
Background: Blood based liquid biopsy has proved its potential in enormous clinical applications, such as cancer screening, diagnosis, treatment guidance, disease tracking and monitoring. In certain scenario (e.g., molecular residual disease), it requires the technique to be able to detect mutation with very low frequency (0.001% ~ 1%). The major hurdle of ultra-sensitive circulating tumor DNA sequencing is the high background noise of plasma cell-free DNA (cfDNA) and clonal hematopoiesis (CH). Here in this study, we investigated the prevalence of CH in lung cancer patients and its interference with liquid biopsy. Methods: We retrospectively analyzed cfDNA and blood cell genomic DNA (gDNA) sequencing data sets (n=1261) from a group of Chinese lung cancer patients. Threshold (1%) and subthreshold (0.2%) for variant allele frequency were set and compared. We focused on 23 clonal hematopoiesis genes that were selected based on previous publications. Results: CH mutations were detected in 27.68% of all the patients at the threshold and 62.01% at the subthreshold, and the detection rate increased with age. DNMT3A was the most frequently mutated CH gene, accounted for more than half of the CH mutations. The CH mutations had a higher detection rate in smokers (72%) than non-smokers (59.4%) at subthreshold. VAFs of CH mutations in cell-free DNA strongly correlated with their VAFs in gDNA (Pearson’s R =0.92, p<2.2x10-16), while tumor derived somatic mutations didn’t have such correlation. Conclusion: Our study showed that clonal hematopoiesis is very common in lung cancer patients, especially when examining low frequency mutations. Sequencing of gDNA at equivalent depth is very important to filter out CH mutation in cancer liquid biopsy.
Functional Finishing of Polyester Fabric With Polystyrene-acrylic/zno Nanocomposite for Effective Dyes Filtration
Authors: Pasichnyk Mariia , Václavíková Miroslava , Gaálová Jana , Melnyk Inna , Minarik Peter 
Published: September 2021

Abstract
Organic dyes such as Methyl Orange (MO) and Methylene Blue (MB) are widely used in different industries, becoming one of the leading emerging water contaminants. It is urgent to develop the most suitable materials for effective elimination of the dyes as they are non-biodegradable and are not removed efficiently by the traditional treatment methods. The present work applied polystyrene-acrylic/ZnO nanocomposite on the polyester surface by blade coating and one-bath pad methods. Features of surface chemical modifications were determined by FTIR, SEM analysis, WCA, N2 adsorption-desorption isotherms. The functionalised layer can create unprecedented function of filter textile material depending on the way of treatment. The possibility of using such fabrics as ﬁlters was explored for MO and MB in the concentration of 100 ppm. Water purification occurred by 60% from methylene blue and 40% from methylene orange flowing through the padded polyester filter and the covered polyester filter for 2 min, respectively. Moreover, it was shown that a colourless reduced form of MB – leuco-methylene blue (LMB) could be created. The functionalised layer of the developed filters through hydrogen bonding between the –OH groups of styrene-acrylic molecules and the –N(CH3)2 groups on LMB can stabilise LMB.
Single-center Retrospective Analysis of One-stop Hybrid Surgery for Brain Arteriovenous Malformations
Authors: Ma Yihui , Wang Lesheng , Shi Jichun , Dong Lixin , Zhang Tingbao , Feng Yu , Gong Yan , Chen Xinjun , Zhao Wenyuan , Chen Jincao 
Published: September 2021

Abstract
Objectives To evaluate the safety and effectiveness of one-stop hybrid surgery of microsurgical resection combined with preoperative embolization or intraoperative angiography for brain arteriovenous malformations (bAVMs) and compare the efficacy of one-stop hybrid surgery with nonhybrid surgery (endovascular embolization alone) in the treatment of bAVMs. Methods A single-center retrospective study was performed to recruit 70 patients with bAVMs at our hospital between July 2017 and April 2020. Patients were divided into 2 groups depending on the mode of surgery: hybrid group (i.e., microsurgical resection combined with preoperative endovascular embolization or intraoperative angiography in the hybrid operating room), and nonhybrid group (i.e., endovascular embolization, stereotactic radiosurgery, or microsurgical resection alone). The hybrid group was divided into two subgroups: the microsurgical resection combined with preoperative embolization, and the microsurgical resection combined with intraoperative angiography subgroups. All patients’ demographic variables, clinical manifestations, and imaging features, postoperative complications, and long-term clinical prognosis were recorded and analyzed. Results Among 70 patients, 48 (68.6%) of whom presented cerebral hemorrhage due to bAVMs rupture. 36 (51.4%) patients in the hybrid group were treated with the combination of pre-operative embolization or intraoperative angiography combined with microsurgical resection, whereas 34 (48.6%) in the nonhybrid group underwent embolization alone. There was no statistical difference in gender, age, ruptured bAVM rate, Spetzler-Martin grades between the 2 groups. Regular clinical and radiological follow-up examinations cure rates were 94.4% in the hybrid group compared to 38.3% in the nonhybrid group (P<0.01). The percentage of patients with favorable outcomes was 94.1% in the hybrid group and 90.6% in the nonhybrid group, although this difference was not statistically significant. Conclusions One-stop hybrid surgery could be a safe and effective intervention to treat bAVMs clinically, whereas further follow-up is needed to determine the long-term effects after operation.
Investigating effect of keyboard distance on the posture and 3D moments of wrist and elbow joints using OpenSim
Authors: Gholami Milad , Choobineh Alireza , Abdoli-Eramaki Mohammad , Dehghan Azizallah , Karimi Mohammad Taghi 
Published: September 2021

Abstract
Background

Musculoskeletal disorders (MSDs) of the upper extremities and computer use are common in modern societies, and both show a growing trend. This study was conducted to determine the posture and 3D moments of wrist and elbow joints at different keyboard distances on a desk.
Methods

Twelve healthy right-handed male volunteers attended the motion analysis laboratory. A keyboard was placed at three different distances from the participants' bodies while performing a standard computer task. The workstation was adjusted according to ANSI/HFES-100-2007 standard for each participant to maintain comfortable ergonomic posture for controlling confounding variables. Qualisys motion capture system, OpenSim software (Ver. 4.1), and visual analog scale (VAS) were used to collect and analyze the data. Also, SPSS (Ver.16) was used to descriptive, Friedman and Wilcoxon tests.
Results

The highest levels of wrist flexion and radial deviation as well as elbow flexion and pronation were observed when the keyboard was at the edge of the desk. When the keyboard was 8 cm away from the edge of the desk, the wrist flexion, deviation, and pronation had the least values. With increasing the distance of the keyboard from the edge of the desk, the range of motion (ROM) of the wrist flexion and deviation as well as elbow flexion, supination and pronation decreased. The joint moments were not significantly different for the different studied keyboard positions (P-value > 0.05).
Conclusions

However, a cut-off point has to be specified because large keyboard distances cause high extension and flexion of the limbs. The position of the keyboard relative to the body is an important parameter in computer work and has a significant impact on the posture of the upper extremities. A keyboard should be located at a distance that allows the upper extremities to remain in a neutral position so that the risk of MSDs is reduced.
Anti-proliferative effects of the combination of Sulfamethoxazole and Quercetin via caspase3 and NFkB gene regulation: An in vitro and in vivo study
Authors: Sahyon Heba , Ramadan Eman N.M. , Althobaiti Fayez , Mashaly Mohammad M.A. 
Published: September 2021

Abstract
Combination therapy comprising natural polyphenols and anticancer drugs has been used to decrease the adverse effects and increase the effectiveness and antioxidant activities of the drugs. The antioxidant and anticancer effects of quercetin (Q), a nutritive polyphenol, have been observed both in vitro and in vivo. Likewise, the anticancer activity of sulfamethoxazole (S) has been demonstrated in vitro and in vivo. This study aimed to investigate the in vitro and in vivo anticancer effects of Q alone and in combination with S. The in vitro effects of S, Q, and S + Q on HCT-116, HepG2, MCF-7, and PC3 cell lines were examined. Additionally, the in vivo effects of these drugs were evaluated using Ehrlich ascites carcinoma (EAC) tumor-bearing mice. The in vitro data revealed the potent anticancer activity of S + Q through the induction of apoptosis and cell cycle arrest. The EAC-inoculated mice treated with S + Q presented with elevated SOD, GSH, CAT, and TAC levels and decreased malondialdehyde levels compared with the untreated EAC group, thus revealing the antioxidant and protective actions of S + Q against EAC cells invasion. Furthermore, the downregulation of NFkB and upregulation of the caspase3 gene in the EAC-inoculated mice treated with the S + Q indicated the induction of the apoptotic pathway and decrease in both cell proliferation and metastasis. In conclusion, the combination of S and Q might exert anticancer effects by inducing apoptosis and exhibiting selective toxicity against the cancer cells and thereby protecting the vital organs.
Reduction the Nanoparticles Generated at the Wheel–rail Contact by Applying Tap Water Lubricant at Subway Train Operational Velocities1
Authors: Lee HyunWook 
Published: September 2021

Abstract
The formation characteristics and reduction of nanoparticles emitted from wheel–rail contacts at subway train velocities of 73, 90, and 113 km/h under dry and water-lubricated conditions (using tap water) were studied using a twin-disk rig. The resulting number concentration (NC) of ultrafine and fine particles increased with train velocity under both conditions. Particle generation varied with slip rate under both conditions in both the particle categories studied. Further, the formation characteristics at 113 km/h under dry conditions showed a notable deviation from those under water-lubricated conditions in three aspects: (i) the maximum NC of ultrafine particles was higher than that of fine particles, (ii) the predominant peak diameter was in the ultrafine particles category, and (iii) the proportion of ultrafine particles was much higher than those of fine particles. Applying water decreased the NC of ultrafine and fine particles significantly at all tested velocities (by 54%–69% and 87%–91%, respectively). Adding water increased the NC of particles ≤35 nm in diameter, possibly owing to the increase in water vapor and mineral crystals from tap water. Overall, this study provides a reference for researchers aiming to minimize nanoparticle formation at the wheel–rail contacts by applying a lubricant.
BioRssay: An R Package for Analyses of Bioassays and Probit Graphs
Authors: Karunarathne Piyal , Pocquet Nicolas , Labbé Pierrick , Milesi Pascal 
Published: September 2021

Abstract
Dose-response relationships reflect the effects of a substance on organisms, and are widely used in broad research areas, from medicine and physiology, to vector control and pest management in agronomy. Furthermore, reporting on the response of organisms to stressors is an essential component of many public policies (e.g. public health, environment), and assessment of xenobiotic responses is an integral part of the World Health Organization recommendations. Building upon an R script that we previously made available, and considering its popularity, we have now developed a software package in the R environment, BioRssay, to efficiently analyze dose-response relationships. It has more user-friendly functions, more flexibility, and proposes an easy interpretation of the results. The functions in the BioRssay package are built on robust statistical analyses to compare the dose/exposure-response of various bioassays and effectively visualize them in probit-graphs.
Study of Lithium Disilicate Based Nano Glass Ceramics Containing P2O5.
Authors: Deshpande Anuradha Vilas , Satyanarayana Peddy 
Published: September 2021

Abstract
Multi component Lithium disilicate based glasses containing P2O5 have been synthesized by conventional melt quenching technique. The replacement of (Li2O+SiO2) by P2O5 and its nucleating effect has been discussed. Structural features of glasses were evaluated by DTA, FTIR and Raman spectroscopy. The glass samples have been converted into glass ceramics by following three stage heat treatment schedule. XRD, FESEM, HAADF imaging and EDX analysis has been carried out for glass ceramics. Vickers microhardness and Vickers indentation fracture toughness of all the glass ceramics have been measured. UV–Visible spectroscopy study has been carried out for glass ceramics to investigate the optical properties. The glass ceramic with 1.5 mol % P2O5 has highest transmittance. Glass ceramics with P2O5 ≥1 mol % having Li2Si2O5 as main phase exhibit high Vickers microhardness (Hv) about 6.71-6.82 GPa which is suitable for dental and armor applications.
Photo-induced Antiferromagnetic-ferromagnetic and Spin-state Transition in GdBaCo2O5.5 Thin Film
Authors: Zhang Yujun , Katayama Tsukasa , Chikamatsu Akira , Schuessler-Langeheine Christian , Pontius Niko , Hirata Yasuyuki , Takubo Kou , Yamagami Kohei , Ikeda Keisuke , Yamamoto Kohei , Hasegawa Tetsuya , Wadati Hiroki 
Published: September 2021

Abstract
Investigation of ultrafast dynamic behaviors can provide novel insights about the coupling mechanisms among multiple degrees of freedom, such as lattice, magnetism and electronic structure, in condensed matters. Here we investigate both the ferromagnetic (FM) and antiferromagnetic (AFM) ultrafast dynamics of a strongly correlated oxide system, GdBaCo2O5.5 thin film by time-resolved x-ray magnetic circular dichroism in reflectivity (XMCDR) and resonant magnetic x-ray diffraction (RMXD). A photo-induced AFM-FM transition characterized by an increase of the transient XMCDR (sensitive to FM order) beyond the unpumped value and a decay of RMXD (sensitive to AFM order) was observed. The photon-energy dependence of the transient XMCDR and reflectivity could be interpreted as a concomitant photo-induced spin-state transition (SST). The AFM-FM transition and SST couple with each other in the time domain, resulting in unusual dynamic behaviors of the magnetism.
Amino acid feeding reduces ammonia production through rearrangement of metabolic fluxes in central carbon metabolism of CHO cells
Authors: Savizi Iman Shahidi Pour , Maghsoudi Nader , Motamedian Ehsan , Lewis Nathan E. , shojaosadati seyed abbas 
Published: September 2021

Abstract
Ammonia is a toxic byproduct of CHO cell metabolism, which inhibits cell growth, reduces cell viability, alters glycosylation, and decreases recombinant protein productivity. In an attempt to minimize the ammonium accumulation in cell culture media, different amino acids were added individually to the culture medium before the production phase to alleviate the negative effects of ammonium on cell culture performance. Among all the amino acids examined in this study, valine showed the most positive impact on CHO cell culture performance. When the cultured CHO cells were fed with 5 mM valine, EPO titer was increased by 25% compared to the control medium, and ammonium and lactate production were decreased by 23 and 26%, respectively, relative to the control culture. Moreover, the sialic acid content of the EPO protein in valine-fed culture was higher than in the control culture, most likely because of the lower ammonium concentration. Flux balance analysis (FBA) results demonstrated that the citric acid cycle was enriched by valine feeding. The measurement of TCA cycle activity supported this finding. The analysis revealed that there might be a link between promoting tricarboxylic acid (TCA) cycle metabolism in valine-fed culture and reduction in lactate and ammonia accumulation. Furthermore, in valine-fed culture, FBA outcomes showed that alanine was excreted into the medium as the primary mechanism for reducing ammonium concentration. It was predicted that the elevated TCA cycle metabolism was concurrent with an increment in recombinant protein production. Taken together, our data demonstrate that valine addition could be an effective strategy for mitigating the negative impacts of ammonium and enhancing glycoprotein production in both quality and quantity.
Compressive Strength of High-Volume Fly Ash (HVFA) Concrete as a Function of Lime Water and Curing Time
Authors: Yun Chin Mei , Rahman Md. Rezaur , Kuok Kuok King , Mersal Mohd Elfy , Liing Colin Ngu Ker , Bakri Muhammad Khusairy Bin 
Published: September 2021

Abstract
The compressive strength of high-volume fly ash (HVFA) concrete with varied volume percentages of 40%, 50%, and 60% was examined utilizing low calcium fly ash (Class-F) as a partial replacement for regular Portland cement in this study. On the 7th, 28th, and 56th days, the compressive strength is tested. At the 7th and 28th days, the influence of saturated lime water on the compressive strength of HVFA concrete is evaluated. The inclusion of fly ash as a replacement for Portland cement reduces the compressive strength of the HVFA concrete, according to the findings. At the 56th day, the HVFA concrete with 40% fly ash substitution has a compressive strength equivalent to the regular weight concrete. At the 28th day, it was discovered that saturated lime water was helpful in maximizing the strength of HVFA concrete with 50% fly ash substitution. There was additional information about the characterization of HVFA concrete.