bluuebunny/crossref_metadata_2025_split huggingface dataset:
Barotropic Energy Conversion During Indian Summer Monsoon: Implication of Central Indian Ocean Mode Simulation in CMIP6
Authors: Qin Jianhuang , Zhou Lei , Meng Ze , Li Baosheng , Lian Tao , Murtugudde Raghu 
Published: July 2021

Abstract
The simulation and prediction of the Indian summer monsoon (ISM) and its intraseasonal component in climate models remain a grand scientific challenge for models. Recently, an intraseasonal mode was proposed over the tropical Indian Ocean, named central Indian Ocean (CIO) mode. The CIO mode index and with monsoon intraseasonal oscillations (MISO) have a high correlation. In this study, the simulations of the CIO mode in the sixth phase of the Coupled Model Intercomparison Project (CMIP6) models are examined. Although the coupled ocean-atmosphere feedbacks associated with the CIO mode are not fully reproduced, the results show that a better depiction of the CIO mode in CMIP6 models is favorable for a better simulation of northward-propagating MISO and heavy rainfall during the ISM. Dynamic diagnostics unveil that the rendition of the CIO mode is dominated by kinetic energy conversion from the background to the intraseasonal variability. Furthermore, kinetic energy conversion is controlled by the meridional shear of background zonal winds (\(\frac{\partial \stackrel{-}{u}}{\partial y}\)), which is underestimated in most CMIP6 models, leading to a weak barotropic instability. As a result, a better simulation of \(\frac{\partial \stackrel{-}{u}}{\partial y}\) is required for improving the CIO mode simulation in climate models, which helps to improve the simulation and prediction skill of northward-propagating MISO and monsoonal precipitation.
A Case of Degos Disease Complicated By Constrictive Pericarditis In Remote Phase
Authors: Tadokoro Yuki , Kitamura Tadashi , Horai Tetsuya , Miyaji Kagami 
Published: July 2021

Abstract
Background: Degos disease, also known as malignant atrophic papulosis, is characterized by cutaneous manifestations due to chronic thrombo-obliterative vasculopathy. There have been reports of rare late-onset Degos disease complicated by constrictive pericarditis (CP). We report a case of CP caused by Degos disease that developed 20 years after diagnosis.Case presentation: A 62-year-old woman who has been taking aspirin for 20 years for Degos disease was hospitalized for worsening heart failure. The patient was diagnosed with CP and underwent pericardiectomy. Pathological findings suggested the involvement of Degos disease. The postoperative course was uneventful, and her heart failure and Degos disease did not worsen.Conclusions: This report suggests that Degos disease can cause long-term CP. Aspirin effectively inhibited the progression of Degos disease, and surgical treatment is necessary when heart failure due to CP is refractory to treatment.
The Systematic Development of Attributes And Levels For A Discrete Choice Experiment of HIV Patient Preferences For Long-Acting Antiretroviral Therapies In The United States
Authors: Brah Aaron T. , Barthold Douglas , Hauber Brett , Collier Ann C. , Ho Rodney J.Y. , Marconi Vincent C. , Simoni Jane M. , Graham Susan M. 
Published: July 2021

Abstract
Introduction: Patient preferences for long-acting antiretroviral therapies (LA-ART) should inform development of regimens with optimal adherence and acceptability. We describe a systematic process used to identify attributes and levels for a discrete choice experiment (DCE) designed to elicit preferences for potential LA-ART options in the US. Methods: Our approach was conducted in four stages: data collection, data reduction, removing inappropriate attributes, and optimizing wording. We started with 8 attributes defining potential LA-ART products based on existing literature and knowledge of products in development. We conducted 12 key informant interviews with experts in HIV treatment. The list of attributes, the set of plausible levels for each attribute, and restrictions on combinations of attribute levels were updated iteratively.Results: Despite uncertainty about which products will become available, key informant discussions converged on 4 delivery modes (infusions and patches were not considered immediately feasible) and 6 additional attributes. Treatment effectiveness and frequency of clinical monitoring were dropped. Oral lead-in therapy was split into two attributes: pre-treatment time undetectable and pre-treatment negative reaction testing. We omitted product-specific systemic and local side effects. In addition to mode, the final set of attributes included: frequency of dosing; location of treatment; pain; pre-treatment time undetectable; pre-treatment negative reaction testing; and late-dose leeway.Conclusions: A systematic process successfully captured elements that are both feasible and relevant to evaluating the acceptability of potential LA-ART alternatives to patients.
Engineered Exosomes Derived From Primary M2 Macrophages With Anti-inflammatory And Neuroprotective Properties For The Treatment of Spinal Cord Injury.
Authors: Zhang Chuanjie , Li Daoyong , Hu Hengshuo , Wang Zhe , An Jinyu , Gao Zhanshan , Zhang Kaihua , Mei Xifan , Wu Chao , Tian He 
Published: July 2021

Abstract
Background: Uncontrollable inflammation and nerve cell apoptosis are the most destructive pathological response after spinal cord injury (SCI). So, inflammation suppression combined with neuroprotection is one of the most promising strategies to treat SCI. Engineered exosomes with anti-inflammatory and neuroprotective properties are promising candidates for the implementation of this strategies for the treatment of SCI. Results: By combining nerve growth factor (NGF) and curcumin (Cur), we prepared stable engineered exosomes of approximately 120 nm from primary M2 macrophages with anti-inflammatory and neuroprotective properties (Cur@EXs-cl-NGF). Notably, NGF was coupled with EXs by matrix metalloproteinase 9 (MMP9)-cleavable linker to accurately release at the injured site. Through targeted experiments, we found that these exosomes could actively and effectively accumulate at the injured site of SCI mice, which greatly improved the bioavailability of the drugs. Subsequently, Cur@EXs-cl-NGF reached the injured site and could effectively inhibit the uncontrollable inflammatory response to protect the spinal cord from secondary damage; in addition, Cur@EXs-cl-NGF could release NGF into the microenvironment in time to exert a neuroprotective effect against nerve cell damage. Conclusions: A series of in vivo and in vitro experiments showed that the engineered exosomes significantly improved the microenvironment after injury and promoted the recovery of motor function after SCI. We provide a new method for inflammation suppression combined with neuroprotective strategies to treat SCI.
Spatiotemporal Dynamics in Spiking Recurrent Neural Networks Using Modified-Full-FORCE on EEG Signals
Authors: Ioannides Georgios , Kourouklides Ioannis , Astolfi Alessandro 
Published: July 2021

Abstract
Methods on modelling the human brain as a Complex System have increased remarkably in the literature as researchers seek to understand the underlying foundations behind cognition, behaviour, and perception. Computational methods, especially Graph Theory-based methods, have recently contributed significantly in understanding wiring connectivity of the brain, modelling it as a set of nodes connected by edges. Therefore, the brain's spatiotemporal dynamics can be holistically studied by considering a network, which consists of many neurons, represented by nodes. Various models have been proposed for modelling such neurons. A recently proposed method in training such networks, called full-Force, produces networks that perform tasks with fewer neurons and greater noise robustness than previous least-squares approaches (i.e. FORCE method). In this paper, the first direct applicability of a variant of the full-Force method to biologically-motivated Spiking RNNs (SRNNs) is demonstrated. The SRNN is a graph consisting of modules. Each module is modelled as a Small-World Network (SWN), which is a specific type of a biologically-plausible graph. So, the first direct applicability of a variant of the full-Force method to modular SWNs is demonstrated, evaluated through regression and information theoretic metrics. For the first time, the aforementioned method is applied to spiking neuron models and trained on various real-life Electroencephalography (EEG) signals. To the best of the authors' knowledge, all the contributions of this paper are novel. Results show that trained SRNNs match EEG signals almost perfectly, while network dynamics can mimic the target dynamics. This demonstrates that the holistic setup of the network model and the neuron model which are both more biologically plausible than previous work, can be tuned into real biological signal dynamics.
The Relationship Between Energy Expenditure and Physical Functions in Patients Hospitalised For Stroke
Authors: Hobo Kota , Kurita Hideaki , Momose Kimito 
Published: July 2021

Abstract
We assessed the relationship between energy expenditure (EE) and functional independence measure (FIM-M) score, Berg Balance Scale (BBS), and comfortable walking speed (CWS) in patients hospitalised for stroke. The total EE per day (TEE), EE during rehabilitation (REE), and EE during activities other than rehabilitation (OEE) were measured using a single-axis acceleration sensor in 36 patients hospitalised for a first stroke episode. In addition, the relationships between each type of EE and FIM-M, BBS, and CWS were investigated. In these patients (mean age 66.2±10.6 years), the median values of TEE, REE, and OEE were 41.8 kcal, 18.5 kcal, and 16.6 kcal, respectively. Correlations were observed between each EE type and all physical function indices. Following the stratification of patients into two groups (high and low) based on the level of physical function, a significant correlation between EE type and physical function was observed only in the low BBS group. EE was correlated with overall physical function indices, but the trend differed depending on physical ability. When patients were stratified based on ability, there were several groups with no significant correlation. Therefore, many patients were unable to achieve an appropriate EE for their level of physical function.
Alternative Fistula Risk Score and First Postoperative Day Drain Fluid Amylase as Predictors of Pancreatic Fistula After Pancreatoduodenectomy
Authors: Longo Karina Scalabrin , Bassaneze Thiago , Palma Rogério Tadeu , Waisberg Jaques 
Published: July 2021

Abstract
Objectives: The alternative fistula risk score (aFRS) and the first postoperative day drain fluid amylase (DFA) are predictors of the occurrence of clinically relevant postoperative pancreatic fistula (CR-POPF). No consensus has been reached on which of the scores is a better predictor; moreover, their combined predictive power remains unclear. To our knowledge, this association had not yet been studied.Methods: This study assessed the predictive effect of aFRS and/or DFA on CR-POPF in a retrospective cohort of 58 patients following PD. The Shapiro-Wilk and the Mann-Whitney tests were applied for assessing the distribution of the samples and for comparing the medians, respectively. The receiver operating characteristics (ROC) curve and the confusion matrix were used to analyze the predictive models.Results: The aFRS values were not statistically different between patients in the CR-POPF and non-CR-POPF groups (Mann-Whitney U test: 59.5, p=0.12). The DFA values were statistically different between the CR-POPF and non-CR-POPF groups (Mann-Whitney U test: 27, p=0.004). The aFRS and DFA were independently less predictive for CR-POPF, compared to combined aFRS + DFA.Conclusions: The combined model involving aFRS>20% + DFA≥5,000 U/L was the most effective predictor of CR-POPF occurrence following PD.
Selected by Bioinformatics and Molecular Docking Analysis, Dhea and 2-14,15-Eg are Effective Against Cholangiocarcinoma
Authors: Qin Lei , Kuai Jun , Yang Fang , Yang Lu , Sun Peisheng , Zhang Lanfang , Li Guangpeng 
Published: July 2021

Abstract
Object To identify novel targets for the diagnosis, treatment and prognosis of cholangiocarcinoma, we screen ideal lead compounds and preclinical drug candidates with MYC inhibitory effect from the ZINC database, and verify the therapeutic effect of Dhea and 2-14,15-Eg on cholangiocarcinoma.Methods The gene expression profiles of GSE132305, GSE89749, and GSE45001 were obtained respectively from the Gene Expression Omnibus database. The DEGs were identified by comparing the gene expression profiles of cholangiocarcinoma and normal tissues. GO, KEGG analysis and PPI network analyses were performed. LibDock, ADME and toxicity prediction, molecular docking and molecular dynamics simulations were used to identify potential inhibitors of MYC. Moreover, in vitro, MTT assay, colony-forming assay and the scratch assay were performed to verify the therapeutic effect of Dhea and 2-14,15-Eg.Results PPI network analysis showed that ALB, MYC, APOB, IGF1 and KNG1 were hub genes, of which MYC was mainly studied in this study. A battery of computer-aided virtual techniques showed that Dhea and 2-14,15-Eg have lower rodent carcinogenicity, Ames mutagenicity, developmental toxicity potential, and high tolerance to cytochrome P4502D6, as well as could exist stably in natural circumstances. In vitro assays showed that Dhea and 2-14,15-Eg inhibited cholangiocarcinoma cellular viability, proliferation, and migration via inducing cholangiocarcinoma cells apoptosis.Conclusion This study suggested that Dhea and 2-14,15-Eg were novel potential inhibitors of MYC targeting, as well as are a promising drug in dealing with cholangiocarcinoma and have a perspective application.
The Value of Metagenomic Next -Generation Sequence In Acquired Immunodeficiency Syndrome With Opportunistic Infections
Authors: Liu Li , Yuan Mingjuan , Sun Siqing , Wang Jinrong , Shi Yi , Su Xin 
Published: July 2021

Abstract
Background: Metagenomic next-generation sequence (mNGS) is an emerging powerful pan-pathogen test for the diagnosis of infectious diseases. However, the application of mNGS in acquired immunodeficiency syndrome with opportunistic infections is limited to clinical cases reports and central nervous system infection.In this study, we evaluated the diagnostic value of mNGS in acquired immunodeficiency syndrome(AIDS) with opportunistic infections(OIs).Methods: From January 2018 to February 2021,86 cases were enrolled in this retrospective analysis. All patients underwent mNGS. Clinical data were recorded.Result: In the present study, mNGS identified 76 of 86 infection cases (88.37%).Human betaherpesvirus 5 (CMV) (40.70%), Human gammaherpesvirus 4 (EBV) (40.70%),pneumocystis (31.40%) were the most common pathogens detected. The sensitivity of mNGS (88.37%,76/86) was higher than that of culture (22.10%, 19/86),smear(7%,6/86) and PCR(46.51%,40/86).In the detection of viruses such as (CMV and EBV), the consistency between PCR and mNGS of CMV and EBV was 100%,73.33% respectively. All PCP cases were detected by mNGS. The consistency in detection of talaromyces between culture and mNGS was 75%.mNGS is superior to the common methods such as culture and smear in the detection of mycobacterium tuberculosis and non-mycobacterium tuberculosis. The mNGS findings led to changes in treatment strategies in 47/86 (54.65%) cases. Compared with the patients’treatment before mNGS, patients had lower rate of broad-spectrum antibiotic drugs use during clinical treatment after mNGS 78/86(90.70%) vs 34/86(39.53%)（P<0.0001). Conclusion: mNGS showed a satisfying diagnostic performance in acquired immunodeficiency syndrome with opportunistic infections. mNGS may lead to a more precise antimicrobial treatment and reduced the use of antibiotic medicine.
RBM17 Mediates Evasion of Pro-Leukemic Factors from Splicing-coupled NMD to Enforce Leukemic Stem Cell Maintenance
Authors: Liu Lina , Vujovic Ana , Deshpande Nandan , Sathe Shashank , Anande Govardhan , Xu Joshua , Minden Mark , Yeo Gene , Unnikrishnan Ashwin , Hope Kristin , Lu Yu 
Published: July 2021

Abstract
Chemo-resistance in acute myeloid leukemia (AML) patients is driven by leukemic stem cells (LSC) resulting in high rates of relapse and low overall survival. Here, we demonstrate that upregulation of the splicing factor, RBM17 preferentially marks and sustains LSCs and directly correlates with shorten patient survival. RBM17 knockdown in primary AML cells leads to myeloid differentiation and impaired colony formation and in vivo engraftment. Integrative multi-omics analyses show that RBM17 repression leads to inclusion of poison exons and production of nonsense-mediated decay (NMD)-sensitive transcripts for pro-leukemic factors and the translation initiation factor, EIF4A2. We show that EIF4A2 is enriched in LSC and its inhibition impairs primary AML progenitor activity. Proteomic analysis of EIF4A2-depleted AML cells shows recapitulation of the RBM17 knockdown biological effects, including pronounced suppression of proteins involved in ribosome biogenesis. Overall, these results provide a rationale to target RBM17 and/or its downstream NMD-sensitive splicing substrates for AML treatment.
N Balance and Requirements Under Different N Management Practices in a No-Till Perennial Rice Cropping System
Authors: Huang Guangfu , Zhang Yujiao , Zhang Shilai , Zhang Jing , Gan Shuxian , Sun Tao , Hu Fengyi 
Published: July 2021

Abstract
Aims: In the absence of tillage, perennial rice is an innovation and supplement to rice production. Evaluating crop N uptake and N requirements and maintaining soil N balance are essential for informing decisions regarding optimal N management and the accessibility of the soil environment benefits of perennial rice cropping systems. Methods: To assess the soil nitrogen cycle and balance, formulate optimal N fertilizer management for perennial rice, a field experiment with four nitrogen rates (N0, N1, N2 and N3 refer to 0, 120, 180 and 240 kg N ha-1, respectively) integrated with three planting densities (D1, D2 and D3 refer to 100×103, 167×103 and 226×103 plants ha-1, respectively) was conducted for two years over four seasons (2016-2017) in southern China. Results: The results showed that N2D3 mode could sustainably produce higher dry matter accumulation (15.15 t ha-1) and grain yields (7.67 t ha-1) over four seasons, showed significantly higher N uptake (201 kg ha-1 each season) and less soil N loss (27.1%). Additionally, the N2D3 mode could reach the optimal N balance (-0.2 kg ha-1) in perennial rice fields with low N requirements (23.9 kg N Mg-1 grain), resulting in higher N use efficiency (NAE: 26.5 kg N kg-1, NRE: 64.9%). Conclusion: In the perennial rice cropping system, 180 kg N ha-1 integrated with 226×103 plants ha-1 resulted in higher grain yields with lower N requirements, higher N use efficiencies, and lower soil N losses, thereby maintaining the soil N balance for sustainable perennial rice production.
Albumin Levels as an Independent Risk Factor for Adverse Outcomes in COVID-19 Patients: A Multicenter Restrospective Study.
Authors: xie congyi , Wang Sijiao , Zhou Jian , Shao Changzhou 
Published: July 2021

Abstract
Backgroud: Studies of risk factors for prognosis of COVID-19 have increased rapidly, but researches about the association between albumin (ALB) levels and COVID-19 clinical outcomes are limited. This study aimed to investigate the relationship between admission albumin levels and adverse outcomes in patients with COVID-19.Methods: This study retrospectively-analyzed 199 COVID-19 patients come from five designated hospitals in Fujian Province between January 22 and February 27, 2020. Clinical characteristics and admission laboratory values were collected. Adverse outcomes were defined as meeting at least one of the following criteria: development of ARDS, respiratory failure, shock, MOF, ICU admissions and in-hospital mortality events.Results: After adjusting for potential confounders (age, sex, BMI, current smoking, hypertension, cardiovascular disease, pulmonary disease, tumor, chronic liver disease, D-dimer, creatinine, CK, leukocytes, neutrophil, LDH, BUN and fibrinogen), a non-linear relationship was detected between ALB and adverse comes, which had an inflection point of 32.6. The odds ratio and the confidence intervals on the left and right sides of the inflection point were 0.204 (0.061 to 0.681) and 0.908 (0.686 to 1.203), respectively. Conclusion: The relationship between ALB and adverse outcomes is non-linear. ALB was negatively correlated with adverse outcomes when ALB was less than 32.6.
Maximum Lesion-To-Contralateral Normal Brain Tissue Ratio of 11C-Methionine PET as a Prognostic Imaging Biomarker for Newly Diagnosed and Untreated Astrocytic Glioma
Authors: Nakajo Kosuke , Uda Takehiro , Kawashima Toshiyuki , Terakawa Yuzo , Ishibashi Kenichi , Tsuyuguchi Naohiro , Tanoue Yuta , Nagahama Atsufumi , Koh Saya , Uda Hiroshi , Sasaki Tsuyoshi , Ohata Kenji , Kanemura Yonehiro , Goto Takeo 
Published: July 2021

Abstract
Purpose: This study aimed whether the uptake of amino tracer positron emissiontomography (PET) can be used as an additional imaging biomarker to estimatethe prognosis of glioma.Methods: Participants comprised 56 adult patients with newly diagnosed and untreated World Health Organization (WHO) gradeⅡ-Ⅳ astrocytic glioma who underwent surgical excision and were evaluated by 11C-methionine PET prior to the surgical excision at Osaka City University Hospital from July 2011 to March 2018. Clinical and imaging studies were retrospectively reviewed based on medical records at our institution.Results: Preoperative Karnofsky Performance Status (KPS) only influenced progression-free survival (PFS) (hazard ratio [HR] 0.20; 95% confidence interval [CI] 0.10-0.41, p<0.0001), whereas histology (anaplastic astrocytoma: HR 5.30, 95%CI 1.23-22.8, p=0.025; glioblastoma: HR 11.52, 95%CI 2.27-58.47, p=0.0032), preoperative KPS≥80 (HR 0.23, 95%CI 0.09-0.62, p=0.004), maximum lesion-to-contralateral normal brain tissue (LN max)≥4.03 (HR 0.24, 95%CI 0.08-0.71, p=0.01), and isocitrate dehydrogenase (IDH) status (HR 14.06, 95%CI 1.81-109.2, p=0.011) were factors influencing overall survival (OS) in multivariate Cox regression. OS was shorter in patients with LN max≥ 4.03 (29.3 months) than in patients with LN max<4.03 (not reached; p=0.03). OS differed significantly between patients with IDH mutant/LN max<4.03 and patients with IDH mutant/LN max ≥4.03.Conclusions: LN max using 11C-methionine PET may be used in prognostic markers for newly identified and untreated WHO gradeⅡ-Ⅳ astrocytic glioma.