bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Prognostic Value of LDH And ADA in Serum and Pleural Fluid of Non-Small Cell Lung Cancer
Authors: Zhao Shuang , Zeng Jun , Wu Juanli 
Published: October 2021

Abstract
Objective: To explore the value of dehydrogenase (LDH) and adenosine deaminase (ADA) in serum and pleural fluid in predicting the prognosis of non-small cell lung cancer.Methods: A total of 134 patients with non-small cell lung cancer (NSCLC) with pleural effusion were enrolled. LDH in Serum and LDH and ADA in pleural fluid were detected, and the cancer ratio (serum LDH/pleural fluid ADA ratio) was calculated. Patients were followed until death. To analyze the correlation between the above indicators and the clinical pathological characteristics of the patients, and the predictive value for the prognosis of the patients.Results: Among the included NSCLC patients with pleural effusion, the average age was 61.76 years old, mainly male (66.7%), the right lung was more common (47.2%), adenocarcinoma was the main (82.1%), and smoking patients accounted for 48.0%. In the diagnosis method, there were 94 cases were diagnosed by pleural fluid exfoliated cells or pleural biopsy. In terms of clinical features, serum LDH is only related to adrenal metastasis (p = 0.000), and pleural fluid LDH is related to the patient's pathological type (p = 0.001). There was no correlation between pleural fluid ADA or cancer ratio and patient gender, lesion location, pathological type, diagnosis method, smoking history, and metastatic location. In terms of prognostic value, patients with ADA < 20U/L and cancer ratio >20 have shorter overall survival and worse prognosis (​​p < 0.0001 and p = 0.0178, respectively). The multivariate Cox regression analysis suggested that the cancer ratio (HR = 1.699, 95%CI: 1.097–2.630, p = 0.017) was an independent risk factor for the poor prognosis of patients.Conclusion: The NSCLC patients with pleural fluid ADA < 20U/L and cancer ratio > 20 combined with pleural effusion had a shorter overall survival and worse prognosis.
Analgesic Outcomes of Tramadol Alone and in Combination with Butorphanol or Flurbiprofen Axetil after Caesarean Section: A Retrospective Study with Propensity Score Matching Analysis
Authors: Yang Guiying , Wu Zhuoxi , Deng Qiangting , liang Yan , Bao Xiaohang , Yan Guangming , Peng Jing , Liu Wenjun , Tan Dan , Li Hong 
Published: October 2021

Abstract
Background: To our knowledge, the Society for Obstetric Anesthesia and Perinatology recommends a multimodal analgesic regimen for caesarean delivery analgesia. Therefore, this study aimed to compare the analgesic effects of tramadol alone and in combination with butorphanol or flurbiprofen axetil after caesarean section.Methods: We retrospectively analysed 2323 women with puerperas who had undergone caesarean section based on the electronic medical records of a teaching hospital in China from January 2018 to January 2020. We collected data on demographic characteristics, perioperative complications, surgical factors, and anaesthesia. We also recorded the postoperative analgesic solution, pain intensity (assessed by the numeric rating scale [NRS]) during the first 48 hours after surgery. Postoperative inadequate analgesia during the postoperative 48 hours was defined as an NRS score ≥4. We also collected data regarding off-bed activity and intestinal function recovery. Analgesic outcomes of three groups were compared using propensity score matching analysis.Results: Compared to the tramadol mixed with butorphanol group (group B), the incidence of inadequate analgesia at rest and while moving was lower in the tramadol combined with flurbiprofen axetil group (group F), and the relative risks (RRs) for women with puerperas suffering from inadequate analgesia at rest and while moving in group F were 0.34 (95% confidence interval [CI]: 0.21–0.54; P<0.001) and 0.24 (95% CI: 0.19–0.31, P<0.001), respectively. The percentage of intestinal function recovery and off-bed activity at 2 days postoperatively was higher in group B than in group F (74.1 versus [vs.] 66.4, P=0.006 and 79.4% vs. 66.4%, P<0.001, respectively). Group F was also associated with a lower incidence of inadequate control of pain at rest and while moving than group T (RR, 0.37 [0.23–0.60], P<0.001, and 0.43 [0.33–0.55], P<0.001, respectively). Group B was associated with a higher incidence of inadequate control of moving pain than group T (RR, 1.78, [1.40–2.26], P<0.001).Conclusions: A combination of tramadol and flurbiprofen axetil could enhance the analgesic effect and be safely used for analgesia after caesarean section, but the combination of tramadol and butorphanol may produce an antagonistic effect.Trial registration: Not applicable.
Comparison of Ocular Trauma Between Normalized and The COVID-19 Epidemic Periods in China: A Multi-Center Cross-Sectional Study
Authors: Zhang Yaxin , Feng Kang , Liao Mengyu , Yan Hua 
Published: October 2021

Abstract
Background: To compare the feature of ocular trauma between normalized period and the COVID-19 epidemic period in China, to provide a scientific basis for prevention and control eye injuries in special times in future.Methods: This study is a multi-center cross-sectional study with 30 participated hospitals involving the China Ocular Trauma Society members. All hospitalized cases who visited the ophthalmology department in participated hospitals with eye injuries during the normalized period (2019) and the COVID-19 epidemic period (2020) were included in this study. Demographic characteristic of cases, date of injury, sites and types of injury were collected.Results: This study involved 13525 (61cases with both eyes) injured cases. There were 7269 (53.74%) eye-injured cases and 6256 (46.26%) eye-injured cases in 2019 and 2020 separately. Compared with 2019, the incidence of ocular trauma in retirees, housewives and unemployed increased with year-on-year of 4.96%, 102.67% and 11.64% among all occupations. In 2020, the incidence of eye injuries decreased in all injury sites except for an increase in home (30.29% year-on-year). The incidence of mechanical eye injuries decreased, while that of non-mechanical eye injuries (Chemical/Thermal/Radiation) increased (47.45% year-on-year). There were 255 (3.50%, 255/7269) and 376 (6.01%, 376/6256) non-mechanical injured cases in 2019 and 2020 (Pearson chi2 = 47.33, P < 0.001) separately.Conclusions: During the COVID-19 epidemic period, the incidence of eye injuries decreased. The prevention and control measures should be focused on the non-mechanical injuries, and the retirees, unemployed and housewives during public events period in future.
Irregular Shape as An Independent Predictor of Prognosis in Patients with Primary Intracerebral Hemorrhage
Authors: Liu Chunyang , Zhang Haopeng , Wang Lixiang , Jiang Qiuyi , Lu Enzhou , Yuan Chao , Liang Yanchao , Sun Zhenying , Xiang Huan , Xu Xun , Sun Jingxian , Fu Bo , Zhao Boxian , Zhang Daming , Chen Xin , Wang Ning , Wang Lu , Yang Guang 
Published: October 2021

Abstract
BACKGROUND AND PURPOSE

The utility of non-contrast computed tomography (NCCT) markers in the prognosis of spontaneous intracerebral hemorrhage (ICH) has been concerned. This study aimed to investigate the predictive value of the computed tomography irregularity shape for poor functional outcomes in patients with spontaneous intracerebral hemorrhage.
PATIENTS AND Methods:

We retrospectively reviewed all 782 patients with intracranial hemorrhage in our stroke emergency center from January 2018 to September 2019. Laboratory examination and CT examination were measured within 24 hours of admission. After three months, the patient's functional outcome was assessed using the modified Rankin Scale (mRS). Multinomial logistic regression analyses were applied to identify independent predictors of functional outcome in patients with intracerebral hemorrhage.
RESULTS

Out of the 627 patients included in this study, those with irregular shapes on CT imaging had a higher proportion of poor outcome and mortality 90 days after discharge (P<0.001). Irregular shapes were found to be significant independent predictors of poor outcome and mortality on multiple logistic regression analysis. Besides, the increase of plasma D-dimer was associated with the occurrence of irregular shape (P=0.0387).
CONCLUSIONS

Patients with irregular shape showed worse functional outcomes after intracerebral hemorrhage. The elevated expression level of plasma D-dimer may be directly related to the formation of irregular shapes.
Reproducibility Between Three-Dimensional Turbo Spin-Echo and Two-Dimensional Dual Inversion Recovery Turbo Spin-Echo for Coronary Vessel Wall Imaging in Kawasaki Disease
Authors: Matsumoto Koji , Yokota Hajime , Yoda Takafumi , Ebata Ryota , Mukai Hiroki , Masuda Yoshitada , Uno Takashi 
Published: October 2021

Abstract
Background: Magnetic resonance vessel wall imaging is desirable for evaluating Kawasaki disease (KD)-associated coronary arterial lesions. Purpose: To evaluate reproducibility of three-dimensional turbo spin-echo (3D-TSE) and two-dimensional dual inversion-recovery turbo spin-echo (2D-DIR-TSE) for coronary vessel wall imaging in KD.Methods: Ten patients were prospectively enrolled. Coronary vessel wall imaging with axial-slice orientation 3D-TSE and 2D-DIR-TSE were acquired for cross-sectional images in aneurysmal and normal regions. Lumen area (LA), wall area (WA), and normalized wall index (NWI) of cross-sectional images were measured in both regions. Reproducibility between 3D-TSE and 2D-DIR-TSE was evaluated via intraclass correlation coefficients (ICCs) and Bland-Altman plots.Results: 48 points (aneurysmal, 27; normal, 21) were evaluated. There were high ICCs between 3D-TSE and 2D-DIR-TSE in LA (0.95) and WA (0.95). In aneurysmal regions, 95% limits of agreement were LA, WA, and NWI of -29.9–30.4 mm2, -18.8–15.0 mm2, and -0.22–0.20, respectively. In normal regions, the 95% limits of agreement were LA, WA, and NWI of -4.44–4.38 mm2, -3.51–4.30 mm2, and -0.14–0.16, respectively. No fixed and proportional biases between 3D-TSE and 2D-DIR-TSE images in aneurysmal and normal regions were noted.Conclusions: 3D-TSE was reproducible with conventional 2D-DIR-TSE for coronary vessel wall assessment on KD.
Effect of low-dose ketamine pretreatment to etomidate on serum cortisol levels in critically ill cardiac patients: A Randomized clinical trial
Authors: Elhamamsy Mostafa , Aldemerdash Ahmed Mohammed , Zahran Fathi Badie , Ezz Gehan Fawzy Mahmoud , AlSaud Sara Abou , Boules Maged Labib , Abdelhady Mahdy Ahmed , Hamed Mohamed Ahmed 
Published: October 2021

Abstract
Background: This study aimed to evaluate the effect of low-dose ketamine pretreatment to etomidate on serum cortisol levels in critically ill cardiac patients with acute anemia undergoing upper endoscopy and ColonoscopyPatients and Methods: Sixty adult cardiac patients, ≥ 18 years, who underwent upper endoscopy and Colonoscopy to manage acute anemia in the cardiac intensive care units were enrolled. Patients were randomly divided into two groups: Group (E): received etomidate 0.2 mg/kg IV followed by etomidate 0.05 mg/kg IV, and Group (KE): received ketamine 0.5 mg/kg IV, then etomidate 0.1 mg/kg IV, followed by etomidate 0.05 mg/kg IV. The primary outcome was Serum cortisol level at 6 hours after the procedure. The secondary outcomes: Estimated change in serum cortisol level (6-hours postoperative value compared to preoperative one), the incidence of any adverse effects, patient satisfaction, and total etomidate dose. Results: The mean postoperative cortisol level significantly reduced in group E than group KE; (295.60±49.218) mcg/dL versus (461.00 ±67.946) mcg/dL respectively With 95% CI = 351.94 to 404.66. In addition, the estimated serum cortisol reduction level was also significant between groups; In group E, the estimated cortisol level decreased nearly 53% from 632.40±35.066 mcg/dL to 295.60±49.218 mcg/dL six hours postoperative. While in group KE, the estimated cortisol level decreased only 27% from 639.13±43.035 mcg/dL to 461.00±67.946 mcg/dL.Conclusions: low-dose ketamine pretreatment to etomidate can successfully control etomidate-induced adrenocortical suppression and minimize the decrease in serum cortisol level in critically ill cardiac patients with no additional adverse effects.Trial Registration: This study is registered on ClinicalTrials.gov (NCT04857450; principal investigator: Mostafa Mohammed Elsaid Elhamamsy; registration date: 23/04/ 2021).
Tailoring the sampling time of single-sample GFR measurement according to expected renal function: a multisite audit
Authors: McMeekin Helena , Townrow Sam , Barnfield Mark , Bradley Andy , Fongenie Ben , McGowan Daniel R , Memmott Matthew , Porter Charlotte A , Wickham Fred C , Vennart Nick , Burniston Maria 
Published: October 2021

Abstract
BackgroundThe 2018 BNMS Glomerular Filtration Rate (GFR) guidelines recommend a single-sample technique with the sampling time dictated by the expected renal function, but this is not known with any accuracy before the test. We aimed to assess whether the sampling regime suggested in the guidelines is optimal, and determine the expected error in GFR result if the sample time is chosen incorrectly. We can then infer the degree of flexibility in the sampling regime.Methods Data from 8946 patients referred for GFR assessment at 6 different hospitals for a variety of indications were reviewed. The difference between the single-sample (Fleming) GFR result at each sample time and the slope-intercept GFR result at each hospital was calculated. A second dataset of 775 studies from one hospital with nine samples collected from 5 minutes to 8 hours post injection was analysed to provide a reference GFR to which the single sample results were compared.Results Recommended single-sample times have been revised: for estimated GFR above 80 ml/min/1.73m2 a 2 hour sample is recommended, giving mean difference from slope-intercept GFR of -2.08 ml/min/1.73m2 (1333 GFR tests included). Between 30 and 80 ml/min/1.73m2 a 4 hour sample is recommended, giving a 1.95 ml/min/1.73m2 mean difference (2057 GFR tests included). The standard deviation of the differences is 3.50 ml/min/1.73m2 at 2 hours and 2.56 ml/min/1.73m2 at 4 hours for GFR results in the recommended range. It is 5.81 ml/min/1.73m2 at 2 hours and 5.70 ml/min/1.73m2 at 4 hours for GFR results outside the recommended range. ConclusionThe results of this multisite study demonstrate a reassuringly wide range of sample times for an acceptably accurate single-sample GFR result. Modified recommended single-sample times have been proposed in line with the results, and the reported errors for both sample times can be used for error analysis of a mistimed sample.
Development of a Cost-effective Biomechanical System for the Assessment of Kinematic and Kinetic Parameters in Soccer Players During a Lower Limb Pointing Task
Authors: SILVA MARCELO GUIMARAES , Rocha Henrique Martins , Katsigianni Cathrine 
Published: October 2021

Abstract
PurposeThe main aim of the present study was to describe the development of a non-commercial biomechanical system designed for the simultaneous measurement of kinematic and kinetic parameters during a lower limb pointing task. The task was performed by two groups of soccer players (10 novices and 10 experts, aged 16-19 years) and the differences between the two groups, in terms of these parameters, were also assessed. MethodsThe calibration procedure of the combined system is presented in this paper. The system measured the Centre of Pressure (CoP) and body Center of Mass (CoM) displacements, kicking duration and the acceleration of the kicking leg. ResultsThe margin of error found for kinematic and kinetic calibration was less than 1%. Furthermore, the expert players presented decreased CoP and CoM displacements and kicking duration and acceleration of the kicking leg compared to the novices. ConclusionThe combined system provides an effective method for the simultaneous measurement of kinematic and kinetic parameters associated with kicking and may contribute to the development of intervention studies for the improvement of balance and kicking performance in sports.
HHi-FiVe: A high-fidelity genetic engineering pipeline for construction of herpesvirus-based vaccines
Authors: Jarvis Michael , Mauch Thekla , Ostermann Eleonore , Wezel Yvonne , Nichols Jenna , Filipe Ana da Silva , Vucak Matej , Hughes Joseph , Henderson Summer , Schmidt Kimberli , Kaur Amitinder , Nichols Hester , Antrobus Robin , Barry Peter , Davison Andrew , Brune Wolfram 
Published: October 2021

Abstract
Herpesvirus-based vectors are attractive for use both as conventional and as transmissible vaccines against emerging zoonoses in hard-to-reach animal populations. However, the threat of off-site mutations during genetic manipulation of vector genomes poses a significant challenge to vaccine construction. Herein, we present the HHi-FiVe (herpesvirus high-fidelity vector) construction pipeline for generating herpesvirus-based vectors by modifying bacterial artificial chromosomes (BACs) and monitoring integrity at each stage by complete genome sequencing. We used this pipeline to repair a highly mutated rhesus cytomegalovirus BAC containing an Ebola virus transgene. The vector derived from this BAC had been shown previously to protect rhesus macaques from lethal Ebola virus challenge by conventional vaccination. Repair of this BAC restored wild-type cellular tropism to the vector, which is essential for transmissible vaccination. Construction of this candidate transmissible vaccine against Ebola virus demonstrates the utility of the HHi-FiVe pipeline for creating precision-made herpesvirus-based vectors.
Application of DNA Barcoding for the Identification of a Traditional Chinese Medicine Shedan
Authors: Zhou Chenggao , Gan Suran , Zhang Jie , Fan Yeqin , Li Bin , Wan Luosheng , Nie Jing , Wang Xiaogang , Chen Jia-Chun 
Published: October 2021

Abstract
Background

Shedan has a long history of application in Traditional Chinese medicine (TCM), however, Shedan from different original source has been indiscriminately used. So far, there is still a lack of an effective tool to differentiate the original source of Shedan medicinal materials, which brings great risk to the safety and effectiveness of clinical applications. Hence, it is imperative to develop a practicable approach to identify Shedan medicinal materials.
Methods

The specificity of two pairs of primers, including Folmer’s universal primers and a pair of originally designed primers COISNFF/COISNFR, was tested to screen the more specific primers for further origin identification of Shedan. A total of 253 fresh snake gallbladder samples from 31 morphologically identified snake species were collected and authenticated. Moreover, 51 fresh snake bile samples and 17 fresh bile samples from five other common domestic poultry and livestock (cattle, chicken, duck, pig and sheep) were collected and distinguished using the more specific primers. Additionally, a total of 195 market Shedan samples randomly selected from 18 batches of Shedan medicinal materials were investigated. Sequence definition was executed by querying sequence similarities in GenBank and the Barcode of Life Data System (BOLD), respectively.
Results

It turned out that the standard COI barcode obtained by COISNFF/COISNFR primers, rather than Folmer’s universal primers, can distinguish all the testing samples from each other in fresh Shedan samples, and COISNFF/COISNFR primers were also specific to snake species and the other four animal species except duck. In terms of market Shedan, 84.6% (165/195) samples can be attributed to 13 snake species from four families and 4.6% (9/195) can be attributed to adulterated chicken species.
Conclusion

The COI-based DNA barcoding was practicable for species identification of Shedan used in traditional Chinese medicine. The original source of current market Shedan, including adulterated species, has been preliminarily clarified, which provides a foundation for quality control of Shedan medicinal materials.
Minimally invasive endoscopic treatment of gastric leiomyosarcoma: A rare case report and review of literature
Authors: kong guixiang , Su xiaolu , Huang xiaojun 
Published: October 2021

Abstract
Gastrointestinal leiomyosarcoma remains relatively rare,accounting for only 1% of gastric tumors,since the use of c-kit staining was first described in 1998 to differentiate gastrointestinal stromal tumors.In this case report,we described the clinical manifestations,endoscopic features, and pathological immunohistochemical features of the gastric leiomyosarcoma.We found that endoscopic ultrasonography has great value in the diagnosis of gastric leiomyosarcoma,and that minimally invasive endoscopic treatment is safe and effective.Particularly,this case highlights that the early-stage lesions can be treated with minimally invasive endoscopic treatment with good tolerance and satisfactory clinical results.Endoscopic submucosal dissection is thus effective for treating gastric leiomyosarcoma,with promising prospects for clinical application.
The Spread of COVID-19 Crisis Communication by German Public Authorities and Experts on Twitter: Quantitative Content Analysis
Authors: Drescher Larissa S , Roosen Jutta , Aue Katja , Dressel Kerstin , Schär Wiebke , Götz Anne 
Published: October 2021

Background
The COVID-19 pandemic led to the necessity of immediate crisis communication by public health authorities. In Germany, as in many other countries, people choose social media, including Twitter, to obtain real-time information and understanding of the pandemic and its consequences. Next to authorities, experts such as virologists and science communicators were very prominent at the beginning of German Twitter COVID-19 crisis communication.

Objective
The aim of this study was to detect similarities and differences between public authorities and individual experts in COVID-19 crisis communication on Twitter during the first year of the pandemic.

Methods
Descriptive analysis and quantitative content analysis were carried out on 8251 original tweets posted from January 1, 2020, to January 15, 2021. COVID-19–related tweets of 21 authorities and 18 experts were categorized into structural, content, and style components. Negative binomial regressions were performed to evaluate tweet spread measured by the retweet and like counts of COVID-19–related tweets.

Results
Descriptive statistics revealed that authorities and experts increasingly tweeted about COVID-19 over the period under study. Two experts and one authority were responsible for 70.26% (544,418/774,865) of all retweets, thus representing COVID-19 influencers. Altogether, COVID-19 tweets by experts reached a 7-fold higher rate of retweeting (t8,249=26.94, P<.001) and 13.9 times the like rate (t8,249=31.27, P<.001) compared with those of authorities. Tweets by authorities were much more designed than those by experts, with more structural and content components; for example, 91.99% (4997/5432) of tweets by authorities used hashtags in contrast to only 19.01% (536/2819) of experts’ COVID-19 tweets. Multivariate analysis revealed that such structural elements reduce the spread of the tweets, and the incidence rate of retweets for authorities’ tweets using hashtags was approximately 0.64 that of tweets without hashtags (Z=–6.92, P<.001). For experts, the effect of hashtags on retweets was insignificant (Z=1.56, P=.12).

Conclusions
Twitter data are a powerful information source and suitable for crisis communication in Germany. COVID-19 tweet activity mirrors the development of COVID-19 cases in Germany. Twitter users retweet and like communications regarding COVID-19 by experts more than those delivered by authorities. Tweets have higher coverage for both authorities and experts when they are plain and for authorities when they directly address people. For authorities, it appears that it was difficult to win recognition during COVID-19. For all stakeholders studied, the association between number of followers and number of retweets was highly significantly positive (authorities Z=28.74, P<.001; experts Z=25.99, P<.001). Updated standards might be required for successful crisis communication by authorities.