bluuebunny/crossref_metadata_2025_split huggingface dataset:
Material Priority Engineered Metal-Polyphenol Networks: Mechanism And Platform For Multifunctionalities
Authors: Cheng Xinxiu , Zhu Yaxin , Lu Ruofei , Zhang Xiaoqiang , Li Na , Zan Xingjie 
Published: December 2021

Abstract
Engineering the surface of materials with desired multifunctionalities is an effective way to fight against multiple adverse factors during the tissue repair process. Recently, metal-polyphenol networks (MPNs) have gained increasing attention because of their rapid and simple deposition process onto various substrates (silicon, quartz, gold and polypropylene sheets, etc.). However, the coating mechanism has not been clarified, and multifunctionalized MPNs remain unexplored. Herein, the flavonoid polyphenol procyanidin (PC) was selected to form PC-MPN coatings with Fe3+, and assembly parameters, including pH, molar ratio between PC and Fe3+, and material priority during coating formation, were thoroughly evaluated. We found that the material priority (addition sequence of PC and Fe3+) had a great influence on the thickness of the formed PC-MPNs. Various surface techniques (e.g., ultraviolet-visible spectrophotometry, quartz crystal microbalance, X-ray photoelectron spectroscopy, atomic force microscopy, and scanning electron microscopy) were used to investigate the formation mechanism of PC-MPNs, and PC-MPNs were further engineered for multifunctionalities (fastening cellular attachment in the early stage, promoting long-term cellular proliferation, antioxidation and antibacterial activity). We believe that these findings could further reveal the coating formation mechanism of MPNs and guide the future design of MPN coatings with multifunctionalities, thereby greatly broadening their application prospects, such as in sensors, environments, drug delivery, and tissue engineering.
Urinary MicroRNA Expression Analysis of miR-1, miR-215, miR-335, Let-7ain Childhood Nephrotic Syndrome
Authors: Priya C.D.Mohana , Venkatesan Vettriselvi , charmine P.Pricilla , Geminiganesan G.Sangeetha , Ekambaram Sudha 
Published: December 2021

Abstract
Background Recently, urinary exosomal miRNAs are gaining increasing attention as their expression profiles are often associated with specific diseases and they exhibit great potential as noninvasive biomarkers for the diagnosis of various diseases. The present study was aimed to evaluate the expression status of selected miRNAs (miR-1, miR-215-5p, miR-335-5p and let-7a-5p) in urine samples from children with NS [steroid sensitive (SSNS)] and [steroid resistant (SRNS)] along with healthy control group.Methods MicroRNA isolation was carried out in urine samples collected from SSNS (100 nos), SRNS (100 nos), and healthy controls (50 nos) using MiRNeasy Mini Kit, followed by cDNA conversion for all the four selected miRNAs using Taqman advanced miRNA cDNA synthesis kit and their expression was quantified by Taqman Advanced miRNA assay kits using Real Time PCR Machine and Rotogen-Q in SSNS and SRNS patients and healthy control subjects.ResultsQuantification of all the four miRNAs (miR-1, mir-215, miR-335, let 7a) were found to be upregulated in both SSNS and SRNS as compared to control group. Further, the comparison of microRNAs within the case groups revealed significant downregulation of three microRNAs - miR-1, miR-215, miR- 335 and upregulation of let-7a in SRNS group as compared to SSNS. The t-test performed for all the four miRNAs was found to be statistically significant. ConclusionsThe aberrant expression of all the four microRNAs in both SSNS and SRNS as compared to healthy subjects may serve as novel biomarkers to distinguish between NS and healthy controls. The differential expression of microRNA let-7a is useful to discriminate SSNS and SRNS.
Longitudinal Relationship of Liver Injury with Inflammation Biomarkers in Covid-19 Hospitalized Patients Using a Joint Modeling Approach
Authors: Diaz Carla , Barrera Lucia , Lopez-Rodriguez Maria , Casar Clara , Vazquez-Agra Nestor , Pernas Hadrian , Marques Ana Teresa , Vidal Martin , Montoya Jonathan , Andrade Ariadna , Fernandez-Castro Ivan , Varela Pablo , Gonzalez-Quintela Arturo , Otero Esteban , Gude Francisco , Cadarso-Suarez Carmen , Tome Santiago 
Published: December 2021

Abstract
Background: The mechanisms underlying liver disease in patients with COVID-19 are not entirely known.Aim: To investigate, by means of novel statistical techniques, the changes over time in the relationship between inflammation markers and liver damage markers in relation to survival in COVID-19.Methods: The study included 221 consecutive patients admitted to the hospital during the first COVID-19 wave in Spain. Generalized additive mixed models were used to investigate the influence of time and inflammation markers on liver damage markers in relation to survival. Joint modeling regression was used to evaluate the temporal correlations between inflammation markers (serum C-reactive protein [CRP], interleukin-6, plasma D-dimer, and blood lymphocyte count) and liver damage markers, after adjusting for age, sex, and therapy.Results: The patients who died showed a significant elevation in serum aspartate transaminase (AST) and alkaline phosphatase levels over time. Conversely, a decrease in serum AST levels was observed in the survivors, who showed a negative correlation between inflammation markers and liver damage markers (CRP with serum AST, alanine transaminase [ALT], and gamma-glutamyl transferase [GGT]; and D-dimer with AST and ALT) after a week of hospitalization. Conversely, most correlations were positive in the patients who died, except lymphocyte count, which was negatively correlated with AST, GGT, and alkaline phosphatase. These correlations were attenuated with age. Conclusion: The patients who died during COVID-19 infection displayed a significant elevation of liver damage markers, which is correlated with inflammation markers over time. These results are consistent with the role of systemic inflammation in liver damage during COVID-19.
Targeted Therapy of Atherosclerosis Vulnerable Plaque By ROS-Scavenging Nanoparticles And MR/Fluorescence Dual-Modality Imaging Tracing
Authors: Dai Yue , Sha Xuan , Song Xiaoxi , Zhang Xiuli , Xing Mengyuan , Liu Siwen , Xu Kai , Li Jingjing 
Published: December 2021

Abstract
Background: Cardiovascular diseases are currently the leading cause of death and disability worldwide, and the key pathological basis is atherosclerosis (AS). Especially, the rupture of vulnerable plaques is the main cause of acute cardiovascular and cerebrovascular events such as myocardial infarction and stroke. Thus, the early identifying and therapy of vulnerable plaques are necessary. Results: In this study, we developed a novel multimodal imaging platform (GPRD) based on Gd doped Prussian blue (GPB) and rhodamine (Rd) to specifically target and identify the vulnerable plaques with the help of dextran sulfate (DS), one of the excellent ligands of scavenger receptor class A (SR-A). It is more important that the nano-enzyme capacity of GPRD NPs realized the elimination of the excessive production of ROS in cells, and the following reduction of ROS-induced oxidative stress, inflammation, apoptosis, and the formation of macrophage-derived foam cells, presenting an inhibition of plaque progress eventually. Conclusions: The ROS-scavenging multimodal imaging nanoprobe provided a new avenue for the diagnosis and treatment of AS vulnerable plaques.
Distribution and Drug Sensitivity of Pathogenic Bacteria in Diabetic Foot Ulcer Patients With Necrotizing Fasciitis at a Diabetic Foot Center in China
Authors: Li Xuemei , Du Zhipeng , Tang Ziwei , Wen Qin , Cheng Qingfeng , Cui Yunhua 
Published: December 2021

Abstract
Background: When a diabetic foot ulcer (DFU) is complicated by necrotizing fasciitis (DNF), this may increase the risk for amputation and mortality, making DNF treatment more complicated, and may eventually lead to amputation and mortality. DNF treatment must include the appropriate antibiotic intervention. However, studies on the distribution and drug sensitivity of pathogenic bacteria in DNF patients remain lacking. This study investigated the distribution and susceptibility of pathogenic bacteria in DNF patients, and provides empirical antibacterial guidance for the clinic.Methods: In a single diabetic foot center, the results from microbial cultures and drug susceptibility tests of patients with DNF from October 2013 to December 2020 were collected and analyzed.Results: A total of 101 DNF patients were included in this study, of whom 94 had positive culture test results. A total of 124 pathogens were cultured, including 76 Gram-positive bacterial strains, 42 Gram-negative bacterial strains, and six fungal strains. Polymicrobial infections accounted for 26.7% and monomicrobial infections accounted for 66.3%. Staphylococcus aureus was the most common bacterium isolated, followed by Enterococcus faecalis and Streptococcus agalactiae. Pseudomonas aeruginosa, Klebsiella pneumoniae, and Proteus mirabilis were the most common Gram-negative bacteria. Thirty-five strains of multi-drug resistant (MDR) bacteria were isolated, representing 28.2% of the total isolates. Gram-positive bacteria were more sensitive to levofloxacin, moxifloxacin, vancomycin, teicoplanin, tigecycline, and linezolid, while Gram-negative bacteria were more sensitive to amikacin, piperacillin/tazobactam, cefoperazone/sulbactam, ceftazidime, cefepime, imipenem, and meropenem. Conclusions: Gram-positive bacteria were the main bacteria isolated from DNF patients. The bacterial composition, the proportion of MDR bacteria among the pathogens, and high risk for amputation should be fully considered in the initial empirical medication, and broad-spectrum antibacterials are recommended.
Cutter Head Approximation Machining Method of Line Contact Spiral Bevel Gear Pairs Based On Controlling Topological Deviations
Authors: Wang Mingyang , Sun Yuehai 
Published: December 2021

Abstract
To improve the meshing performance and increase the bearing capacity and service life of spiral gear pairs, the cutter head approximation machining method based on controlling topological deviations was proposed to solve the problem where line contact spiral bevel gears with tapered teeth depth cannot be directly machined by cutter heads. First, the mathematical model of line contact conjugate flanks was established, and meshing equations and conjugate flank equations of bevel gear pairs were derived. Second, the gear tooth flank was set as the datum tooth flank for priority machining, and the pinion theoretical tooth flank which is fully conjugate with the gear tooth flank and the pinion machining tooth flank matching with the gear were solved. Then, the geometric topological deviations model of the comparison between the pinion machining tooth flank and its theoretical tooth flank can be established. Finally, with the pinion machining tooth flank approaching its theoretical tooth flank as the modification, the additional cutting motions and machining compensation parameters of cutter heads were obtained to control the pinion machining tooth flank deviations and reduce them to the allowable deviations of its theoretical tooth flank. The contact simulation analysis and rolling test verified the correctness of the line contact conjugate flank model and feasibility of the cutter head approximation machining method.
Racial differences in genomic features and new trans-ancestry prognosis subtypes in bladder cancer
Authors: Zhang Baifeng , Jia Peilin , Wang Jiayin , Pei Guangsheng , Wang Changxi , Pei Shimei , Li Xiangchun , Ding Shuai , Zhao Zhongming , Yi Xin , Huang Yi 
Published: December 2021

Abstract
The incidence and survival of bladder cancer vary greatly among different populations but the influence of the associated molecular features and evolutionary processes on its clinical treatment and prognostication remains unknown. Here, we analyze the genomic architectures of over 500 bladder cancer patients from Asian/Black/White populations. We identify novel association between AHNAK mutations and APOBEC-a mutational signature whose activities vary substantially across populations. All significantly mutated genes but only half of arm-level somatic copy number alterations (SCNAs) are enriched with clonal events, indicating large-scale SCNAs as rich sources fostering bladder cancer clonal diversities. The prevalence of TP53 and ATM clonal mutations as well as the associated burden of SCNAs is significantly higher in Whites/Blacks than in Asians. We identify a trans-ancestry prognostic subtype of bladder cancer characterized by: enrichment of non-muscle-invasive patients and muscle-invasive patients with good prognosis, increased CREBBP/FGFR3/HRAS/NFE2L2 mutations, decreased intra-tumor heterogeneity and genome instability and activated tumor microenvironment.
Study on the Mechanism of CircDUSP16 Regulating the Proliferation and Apoptosis of Hepatocarcinoma Cells
Authors: Tang Xiaofei , Xiang Yang 
Published: December 2021

Abstract
Purpose: This study aims to explore the expression of circDUSP16 in liver cancer and its effect on the proliferation and apoptosis of liver cancer cells. Methods: Real-timePCR was used to measure the expression of circDUSP16, miR-136-5p, and YAP1 in HCC tissues and cells. MTT, colony analysis, and apoptosis analysis were performed to determine the progress of HCC cells. The relationship between circDUSP16, miR-136-5p, and YAP1 was verified by using the luciferase gene experiment. Results: The expression level of circDUSP16 in HCC tissue samples was significantly increased and can be used as an independent prognostic factor for the survival of HCC patients. Inhibition of circDUSP16 can inhibit HCC cell viability, colony formation, and invasion potential. Furthermore, inhibition of circDUSP16 can regulate the expression of YAP1 in the Hippo/YAP signaling pathway in HCC by targeting miR-136-5p, thereby affecting cell proliferation and apoptosis, and participating in the progression of HCC disease. Conclusion: The ectopic expression of circDUSP16 can regulate the expression of YAP1 by competitively binding to miR-136-5p, therefore participating in the progression of HCC, which can provide a new therapeutic target for the treatment of HCC.
Atmospheric Pressure Change Measurement: An Observational Study
Authors: Oshakbayev Kuat , Bedelbayeva Gulnara , Mustafin Khalit , Sabir Abdul , Tordai Attila 
Published: December 2021

Abstract
Introduction: The aim of the study was to show an atmospheric pressure change by indirect measurement in hermetically closed vessels during four years of follow-up.Methods: Study design: an observational study. In hermetically sealed elastic bottles with different liquids were measured differences in liquid and air volumes from baseline to final follow-up period. The physical law of flotation was used to measure liquid and air volume above liquid in bottles. To measure liquid and air (above liquid) volumes in each bottle was used the physical law of buoyancy. The follow-up period was four years.Results: The volumes of liquid and air in all bottles were decreased after the follow-up period to 14.38±2.40 and 36.25±3.37 ml, respectively. Air volume in comparison to liquid volume decreased more than two times significantly (P=0.0007) after the follow-up period.Conclusions: Thus, atmospheric pressure increased during the last 4-year follow-up period. Further investigations are needed.
The Effect of the Multi-doping Strategy on Stabilization of the Cubic δ-phase in the Bi2O3-based Solid Electrolyte Systems in Terms of the SOFC Applications
Authors: BALCI Murat , Arı Mehmet , Polat Yasin 
Published: December 2021

Abstract
In this study, the effects of multi-doping strategy on phase stabilization and electrical conductivity for the doped Bi 2 O 3 system were investigated. All solid mixtures were created by solid state reactions according to a certain stoichiometric ratio in atmospheric conditions. The structural, electrical, thermal and surface characterizations of the created samples were performed by x-ray diffraction method (XRD), four point-probe technique (4-PPT), differential thermal analysis/thermo gravimetric analysis (DTA/TGA) and scanning electron microscope (SEM), respectively. From XRD results, it was seen that the fcc δ-phase could be stabilized by using only 1:1:1:2 or 2:2:2:1 dopant content ratio (in here, “1:” is corresponds to” 5%” mole). The other compounds prepared out of this ratios were mixed phase because of the containing both α-phase peaks and δ-phase peaks on their XRD pattern. When the all samples were compared in terms of electrical conductivity at 750 °C, it was observed that the fcc δ-phase stabilized samples exhibited higher conductivity than that of other compounds as expected. The highest electrical conductivity was for the sample, dopant content ratios of which are 1: 1: 1: 2, with 0.014 S.cm -1 at 750 °C and also it had the lowest activation energy (0.51 eV) among all samples. On the other hand, according to the thermal analysis results, it was concluded that phase transition occurred only on the DTA curve of the sample given with dopant content ratios 1:1:1:1 due to presence of endothermic peak on its DTA curve at 729°C during the heating process. Also, for this sample, it was clearly predicted from the electrical conductivity graphs depending on temperature that the phase transition occurred at just that temperature (729 °C) due to the sudden increase in conductivity by indicating phase transition from the α-phase to the cubic δ-phase. The SEM analysis pointed out that grain size decreased as total dopant ratio increased and also the grain boundary changed sharply with the increase in the total dopant ratio.
Psychometric Properties of Screening Questionnaires To Detect Depression In Primary Healthcare Setting In Rural Ethiopia
Authors: Habtamu Kassahun , Berhane Rahel , Medhin Girmay , Hanlon Charlotte , Fekadu Abebaw 
Published: December 2021

Abstract
Background: Much of the research about the validity of depression screening questionnaires is on criterion validity. Evidence is scarce on the concurrent, convergent and construct validity of these measures, particularly from low-income countries. This study aimed to evaluate the psychometric properties of depression screening questionnaires in primary healthcare (PHC) in rural Ethiopia. Methods: A facility-based cross-sectional study was conducted with 587 participants recruited from patients attending three PHC facilities and two Holy water sites. The psychometric properties of five mental health screening questionnaires were evaluated: the nine item Patient Health Questionnaire (PHQ-9), the two item version of PHQ-9 (PHQ-2), a version of PHQ-9 with two added items of irritability and noise intolerance (PHQ-11), the Patient Health Questionnaire-15 (PHQ-15), and the World Health Organization-Five Well-being Index (WHO-5). Clinical diagnosis of depression was ascertained by psychiatrists using a semi-structured interview. We analyzed data using exploratory factor analysis, Spearman’s rank order correlation coefficient (Rho), the Mann Whitney test of the equality of medians, univariate logistic regression and Cronbach’s alpha. Results: PHQ-9, PHQ-11 and WHO-5 were found to be unidimensional, with items in each scale highly loading onto one factor (factor loadings ranging from 0.64 to 0.87). The items of each instrument were internally consistent, with Cronbach’s alpha ranging from 0.72 (PHQ-2) to 0.89 (PHQ-11). Scores for all screening scales were moderately or highly correlated with each other (Rho= 0.58 to 0.98) and moderately correlated with anxiety and disability scores. Median scores of all screening scales were significantly higher in those diagnosed with depression. The association of items measuring emotional and cognitive symptoms with the diagnosis of depression was stronger than the association with items measuring somatic symptoms. Irritability and noise intolerance had higher association with depression diagnosis than PHQ-9 items. Conclusion: Emotional and cognitive symptoms are more useful than somatic symptoms to predict the diagnosis of depression. Irritability and noise intolerance are more useful to detect depression. Future research should focus on testing the unidimensionality of PHQ-9, PHQ-11 and WHO-5 using confirmatory factor analysis; establishing the criterion validity of PHQ-11 and WHO-5; and on assessing test-retest reliability of all the measures.
Barriers and Enablers of Weight Management After Breast Cancer: A Thematic Analysis of Free Text Survey Responses Using the COM-B Model
Authors: Ee Carolyn , MacMillan Freya , Boyages John , McBride Kate 
Published: December 2021

Abstract
BackgroundWeight gain is common after breast cancer. The aim of this study was to identify and describe the barriers to and enablers of successful weight management for women with breast cancer. MethodsThis was a combined inductive and deductive framework analysis of free text responses to an anonymous cross-sectional survey on weight after breast cancer. Women were recruited mainly through the Breast Cancer Network Australia Review and Survey Group. We applied deductive thematic analysis to free text responses to questions on barriers, enablers, research priorities, and one open-ended question at the end of the survey using the Capability, Opportunity, Motivation and Behaviour (COM-B) model as a framework. Subthemes that arose from the inductive analysis were mapped onto the COM-B model framework. Findings were used to identify behaviour change intervention functions. Results133 women provided free text responses. Most women were of Caucasian origin and had been diagnosed with non-metastatic breast cancer, with a mean age of 59.1 years. Women's physical capability to adopt and sustain healthy lifestyle habits was significantly affected by treatment effects and physical illness, and some lacked psychological capability to self-regulate the face of stress and other triggers. Limited time and finances, and the social impact of undergoing cancer treatment affected the ability to control their diet. Frustration and futility around weight management were prominent. However, some women were confident in their abilities to self-regulate and self-monitor lifestyle behaviours, described support from friends and health professionals as enablers, and welcomed the physical and psychological benefits of being active in the context of embracing transformation and self-care after cancer. ConclusionWomen need specific advice and support from peers, friends and families and health professionals. There is a gap in provision of supportive care to empower women to adopt and sustain healthy lifestyles. Environmental restructuring (including financial support), incentivization (creating an expectation of looking and feeling better), persuasion and coercion (aiming to prevent recurrence), and equipping women with specific knowledge and skills, would also facilitate optimal lifestyle behaviours and weight management.