bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Selah pilot study of spiritual, mindfulness, and stress inoculation practices on stress-related outcomes: A non-randomized participant preference control pilot study
Authors: Proeschold-Bell Rae Jean , Eagle David E. , Tice Logan C. , Yao Jia , Rash Joshua A. , Choi Jessica Y. , Stringfield Beth , Labrecque Sofia M. 
Published: November 2021

Abstract
Background

The job-demand-control-support model indicates that clergy are at high risk for chronic stress and adverse health outcomes.
Methods

A non-randomized participant preference design with a control group was used to evaluate the feasibility, acceptability, and range of outcome effect sizes for four potentially stress-reducing interventions: stress inoculation training, mindfulness-based stress reduction (MBSR), the Daily Examen, and Centering Prayer. All United Methodist clergy in North Carolina were eligible and recruited via email to attend their preferred intervention: in-person workshops of one (Daily Examen, Centering Prayer) or two days (stress inoculation training) at retreat centers, or eight weekly online 90-minute sessions (MBSR). Surveys at 0, 3, and 12 weeks assessed symptoms of stress, anxiety, and perceived stress reactivity. Heart rate variability was assessed at baseline and 12 weeks using data from 24h ambulatory heart rate monitoring. A patched-up control group was recruited after recruitment commenced. A subset of participants completed in-depth interviews and reported skill practice using daily text messages. Standardized mean differences with 95% and 75% confidence intervals were calculated for each intervention relative to control to determine the range of effect sizes likely to be observed in a definitive trial.
Results

78 clergy participated in an intervention and 7 provided data as a control group. The daily percentage of participants engaging in stress management practices ranged from 47% (MBSR) to 69% (Examen). Every participant interviewed (n=23) reported that learning content was acceptable and they would recommend their intervention to others. Small-to-large effect sizes on measures of stress and anxiety were observed for Daily Examen, stress inoculation, and MBSR relative to control. Little evidence for change in HRV was observed between intervention and control, with the most favorable effects observed for MBSR. Results were mixed for Centering Prayer.
Conclusions

All four interventions were feasible and acceptable, with the best trends in outcomes for MBSR, although other interventions were promising. Participants welcomed daily text messages reminding them to engage in practice and wore ambulatory heart rate monitoring devices without incident. Three-week survey data did not contribute to study findings. Trial registration: ClinicalTrials.gov trial registration number: NCT04625777, November 12, 2020 (retrospectively registered).
Detection of Escherichia Coli O157:H7 in Imported Meat Products from Saudi Arabian Ports in 2017
Authors: Alhadlaq Meshari Ahmed , Mujallad Mohammed I. , Alajel Suliman M. I. 
Published: November 2021

Abstract
Escherichia coli O157:H7 is a foodborne pathogen, which causes various health conditions in humans, including fatigue, nausea, and bloody diarrhoea, and in some cases, even death. In 2017, 15.71% of the total imported food products in Saudi Arabia were meat-based. India and Brazil are two of the top five countries from where Saudi Arabia imports meat. According to the Saudi Food and Drug Authority, in 2017, at least 562, 280, and 50 samples of imported beef, chicken, and sheep meat, respectively, were tested for the presence of E. coli O157:H7. Amongst these, E. coli O157:H7 was detected in respectively 6.5% and 2.2% of the tested beef meat samples imported from India and Brazil as well as in respectively 6.96% and 3.57% of the tested chicken samples imported from Brazil and Ukraine. Moreover, the pathogen was detected in 2.1% of the tested sheep meat samples imported from India. The present report provides evidence that imported meat can serve as the carrier of E. coli O157:H7, leading to epidemics, within the Kingdom of Saudi Arabia.
An Efficient Ultrasound Assisted Electrospinning Synthesis of a Novel Biodegradable Polymeric Ni-MOF Supported by PVA- Fibrous Network as an Efficient CH4 Adsorbent
Authors: Shahriari Taher , Chauhan Narendra Pal Singh , Sargazi Ghasem , Ebrahimi Ahmad , Hosseinzadeh Alireza 
Published: November 2021

Abstract
In this study, Ni-MOF was synthesized via ultrasound irradiation under optimal conditions, which included an ultrasound power of 370 W, time duration of 20 minutes, and a temperature of 25 oC. The final Ni-MOF nanostructures were immobilized in PVA fibrous polymeric network using under optimal conditions (concentration: 8 ­wt %; the nozzle tip with flow rates of 0.10 mL/h; temperature: 25­ oC and humidity: 22 %; voltages: 20 kV; spinning distance: 12 cm) of electrospinning. To characterize the final composition, various analyses were used. The presence of functional groups in structures was confirmed by FTIR. The Ni-MOF sample was uniformly synthesized on the surface of PVA, as evidenced by SEM and Mapping elemental analysis. The BJH technique validated the effect of the substrate's role in increasing the surface area of the final products. The final products of the Ni-MOF/PVA fibrous network were used as a novel adsorbent in the adsorption of CH4 gas.
Performance of Graduation Examination of Standardized Residency Training in Zhejiang Province, China
Authors: Jiang Ling , Li Ruoqi , Xia Zhijun , Qu Xianguo , Gao Qisheng 
Published: November 2021

Abstract
Background
Standardized residency training is an important part of post-graduation medical education for medical students in China, and the graduation examination is a necessary part to test the effect of residency training and an important measure to guarantee the quality of residency training. This study analyzed the graduation examination results of standardized residency training in Zhejiang Province in 2020.
Methods
This study calculated the pass rates for the practical skills examination and written examination for 5555 examinees and analyzed the relationship between examinees' demographic characteristics and pass rates.
Results
The pass rates for practical skills examination and written examination were 91.8% and 96.5%, respectively. Gender, age, education, and type of organizations were significantly associated with the pass rates. Examinees who retook the examination were less likely to pass the practical skills examination (AOR = 0.266, 95% CI = 0.189-0.377) and the written examination (AOR = 0.043, 95% CI = 0.025-0.074) than examinees who took the examination for the first time. If an examinee did not pass the practical skills examination initially, he or she was less likely to pass the written examination (AOR = 0.172, 95% CI = 0.120-0.249)
Conclusion
The pass rate of graduation examination of standardized residency training was not only influenced by the individual characteristics of the examinees but also related to the level of training hospitals and lead teachers. Therefore, it is necessary to take comprehensive measures to further improve the system of standardized residency training.
High Expression of HSPA8 is A Favorable Prognostic Factor in Colon Cancer
Authors: Liu Yang-yang , He Xiang-biao 
Published: November 2021

Abstract
Background: Colon adenocarcinoma (COAD), a type of colon cancer, is among the most common malignant diseases afflicting people globally. Heat shock protein family, including HSPA8, are involved in tumor progression in numerous human cancers, however the association between HSPA8 and COAD has not been explored.Methods: RNA sequencing data and clinical characteristics from TCGA and GTEx, as well as protein data from CPTAC were obtained for analysis of HSPA8 expression in COAD. Furthermore, functional enrichment analysis of HSPA8-related differentially expressed genes (DEGs) was performed via GO/KEGG, immune infiltration analysis, and protein-protein interaction (PPI) network. In addition, the clinical significance of HSPA8 was analyzed by Kaplan-Meier Cox regression and prognosis assessment. R package was employed to analyze incorporated studies.Results: Compared with normal samples, the expression of HSPA8 increased remarkably in various tumors, including COAD, Moreover, high HSPA8 expression was associated with good prognosis in COAD, as well as subgroups with male, T2-T4 stage, no lymphatic and perineural invasion. (P < 0.05). High HSPA8 was a prognostic factor in Cox regression analysis. The diagnostic efficacy of HSPA8 in COAD analyzed by ROC (AUC: 0.889). Furthermore, a total of 3321 DEGs were identified between the high- and the low-expression group, of which 9 genes were up-regulated and 3312 genes were down-regulated.Conclusions: High HSPA8 expression is a potential biomarker for favorable outcomes in COAD. the results might provide clues and guidance for developing novel diagnosis, targeted therapy and prognosis of HSPA8 in COAD.
Differentiation of Human Umbilical Cord Derived Mesenchymal Stem Cells Into Neural Stem Cells Induced by hPRDX5
Authors: Jin Yuanyuan , Shi Beichen , Fan Qiang , Liu Kun , Fan Shuai , yang zhaoyong 
Published: November 2021

Abstract
Background: The objectives of this study were to investigate the characteristics and capacity of human umbilical cord‑derived mesenchymal stem cells (hUC-MSCs) differentiation into neural stem cells (NSCs) and whether this event enhanced by hPRDX5. Methods and Results: The adherent cells were obtained from umbilical cord of normal full-term newborn by caesarean section under aseptic condition, and cultivated by tissue block culture method. The surface antigen expression profiles of hUC-MSCs were monitored and the multi-directional differentiation potential was identified. Following amplification, the cells of the 4th passage were divided into 5 groups (groups A-E). The morphology was observed under inverted microscope, and the positive expression rate of markers of neural stem cell was detected by immunocytochemical and western blot. Flow cytometry revealed that the hUC-MSCs expressed CD29, CD73, CD90 and CD105, but not CD19, CD34, CD45 or HLA-DR. Treatment with hPRDX5 led to the surface markers of neural stem cells which were positive for Nestin, but negative for NSE and GFAP expression. Conclusions: Thus, the findings of the present study demonstrate that hPRDX5 effectively promotes hUC-MSCs to differentiate into neural stem cells possibly through TLR4 signaling pathway.
Absence of the Z-disc Protein α-actinin-3 Impairs the Mechanical Stability of Actn3KO Mouse Fast- twitch Muscle Fibres without Altering their Contractile Properties or Twitch Kinetics
Authors: Haug Michael , Reischl Barbara , Nübler Stefanie , Kiriaev Leonit , Mázala Davi A.G. , Houweling Peter J. , North Kathryn N. , Friedrich Oliver , Head Stewart I. 
Published: November 2021

Abstract
Background: A common polymorphism (R577X) in the ACTN3 gene results in complete absence of the Z-disc protein α-actinin-3 from fast-twitch muscle fibres in ~16% of the world’s population. This single gene polymorphism has been subject to strong positive selection pressure during recent human evolution. Previously, using an Actn3KO mouse model, we have shown in fast-twitch muscles, eccentric contractions at L0+ 20% stretch did not cause eccentric damage. In contrast, L0+ 30% stretch produced a significant ~40% deficit in maximum force; here we use isolated single fast-twitch skeletal muscle fibres from the Actn3KO mouse to investigate the mechanism underlying this.Methods: Single fast-twitch fibres are separated from the intact muscle by a collagenase digest procedure. We use label-free second harmonic generation (SHG) imaging, ultra-fast video microscopy and skinned fibre measurements from our MyoRobot automated biomechatronics system to study the morphology, visco-elasticity, force production and mechanical strength of single fibres from the Actn3KO mouse. Data are presented as means ± SD and tested for significance using ANOVA.Results: We show that the absence of α-actinin-3 does not affect the unloaded maximum speed of contraction, visco-elastic properties or myofibrillar force production. Eccentric contractions demonstrated that chemically skinned Actn3KO fibres are mechanically weaker being prone to breakage when eccentrically contracted. Furthermore, SHG images reveal disruptions in the myofibrillar alignment of Actn3KO fast-twitch fibres with an increase in Y-shaped myofibrillar lattice shifts. Conclusions: Absence of α-actinin-3 from the Z-disc in fast-twitch fibres disrupts the organisation of the myofibrillar proteins, leading to structural weakness. This provides a mechanistic explanation for our earlier findings that, in vitro intact Actn3KO fast-twitch muscles are significantly damaged by L0+ 30%, but not, L0+ 20%, eccentric contraction strains. Our study also provides a possible mechanistic explanation as to why α-actinin-3 deficient humans have been reported to have a faster decline in muscle function with increasing age, that is; as sarcopenia reduces muscle mass and force output, the eccentric stress on the remaining functional α-actinin-3 deficient fibres will be increased, resulting in fibres breakages.
Investigating the Kinetics of the Thermolysis of 3-nitro-2,4-dihydro-3H-1,2,4-triazol-5-one (NTO) and Reduced Size NTO the Presence of Cobalt Ferrite Additive
Authors: Dave pragnesh N , Sirach Ruksana , Thakkar Riddhi , Deshpande M P 
Published: November 2021

Abstract
Less sensitive high energetic materials (HEMs) are explored as a potential replacement of highly sensitive HEMs in propellants, and explosive applications. More research have been devoted to improve the thermal decomposition of such a less sensitive HEMs. Nanosize Cobalt ferrite (CoF) has been successfully synthesized using the co-precipitation method. Synthesis of less sensitive HEM 3-nitro-2,4-dihydro-3H-1,2,4-triazol-5-one(NTO) and its size reduction using solvent-antisolvent method is successfully achieved. Effect of 5 % by weight CoF on the thermolysis of NTO and NTO with reduced size (r-NTO) has been studied using the simultaneous thermal analysis. Three isoconversional methods namely Flynn Ozawa Wall, Kissinger-Akahira-Sunose (KAS), and Starink are employed to evaluate the kinetic parameter of NTO, and r-NTO in the presence of CoF additive. It was found that both the addition of CoF as well as reducing size of NTO can decrease the thermal decomposition temperature of NTO, the later decreasing the thermal decomposition temperature to a good extent compared to former. However, the kinetic study using isoconversional methods suggested that in the presence of CoF additive, the activation energy of both NTO as well as n-NTO is increased.
Plants for Making Tibetan Traditional Wooden Bowls and Related Traditional Knowledge in the Gyirong Valley, Tibet, China
Authors: Ding Xiaoyong , Guo ChangAn , Hu HuaBin , Wang Yuhua 
Published: November 2021

Abstract
Background: The wooden bowl is an important symbol of the Tibetan cultures, yet, in China, little has been documented regarding the raw materials used to make these items as well as their cultural significance in Tibet. This study explores the ethnobotanical uses of plants used to make wooden bowls to understand their sustainability, cultural significance, and current status of related traditional knowledge in Gyirong Town, which is one of the most famous places for wooden bowl making.Materials and methods: Between 2019 and 2021, key informant interviews, semi-stuctured interviews, and participatory observations were used to conduct ethnobotanical field surveys in Gyirong Valley. The field work was performed with the assistance of local guides. In this study, we utilised a use-report (UR) and relative frequency citation (RFC) to evaluate the value and importance of the species used to make the wooden bowls..Results: Our results show that 16 different plants are used during the wooden bowl making process, of which nine are used as raw materials, three for dyeing, and four for varnishing. Although communities rely heavily on these plants, good management and collection methods were observed. We also recorded the use of Fallopia denticulata as a red dye and four species of Impatiens as wood lacquers for the first time.Conclusion: We found no significant difference in the knowledge of the traditional use of plants among people of different ages and genders in Gyirong; traditional knowledge is widespread among family members and among different villages. The government and local people are committed to the protection and development of traditional knowledge related to wooden bowls, and this knowledge maintains a healthy degree of vitality. This research can provide insights into the vitality of traditional handicrafts that are facing challenges and promote their protection.
Net carbon sink of China was overestimated by more than 35%
Authors: Du Chaochao , Bai Xiaoyong , Li yangbing , Tan Qiu , Zhao Cuiwei Zhao , Luo Guangjie , Wu Luhua , Chen Fei , Li Chaojun , Ran Chen , Luo Xuling , Xi Huipeng , Chen Huan , Zhang Sirui , Li Mi , Gong Suhua , Xiong Lian , Song Fengjiao , Xiao Biqin 
Published: November 2021

Abstract
As a carbon source/sink of atmospheric carbon dioxide, the net regional carbon budget (NRCB) of terrestrial ecosystems is very important to effect global warming, especially China with the largest emissions at present. However, the carbon consumption is difficult to measure accurately, which is caused by the emissions of CH4 and CO, the utilization of agriculture, forestry and grass, and the emissions from rivers and other physical processes, such as forest fires. Therefore, the spatial patterns and driving factors of NRCB are not clear. Here, we used multi-source data to estimate the NRCB of 31 provincial administrative divisions of China and to develop NRCB datasets from 2000 to 2018. We found that the average of NRCB was 669 TgC yr−1, and it significantly decreased at a rate of 2.56 TgC yr−1. The relative contribution rates of fluxes of emissions from anthropogenic (FEAD), reactive carbon and creature ingestion (FERCCI), autotrophic respiration (Ra), heterotrophic respiration (Rh) and natural disturbances (FEND) were 35.17%, 26.09%, 19.68%, 17.38% and 1.68% respectively. In addition, NRCB datasets of the different administrative regions of China were mapped. These datasets will provide support for China's carbon neutrality and the study of the global carbon cycle.
Comparison Between Microendoscopic Laminectomy and Open Posterior Decompression Surgery for Single-Level Lumbar Spinal Stenosis: A Multicenter Retrospective Cohort Study
Authors: Ohtomo Nozomu , Nakamoto Hideki , Miyahara Junya , Yoshida Yuichi , Nakarai Hiroyuki , Tozawa Keiichiro , Fukushima Masayoshi , Kato So , Doi Toru , Taniguchi Yuki , Matsubayashi Yoshitaka , Higashikawa Akiro , Takeshita Yujiro , Kawamura Naohiro , Inanami Hirohiko , Tanaka Sakae , Oshima Yasushi 
Published: November 2021

Abstract
BackgroundMicroendoscopic laminectomy (MEL), in which a 16-mm tubular retractor with an internal scope is used, has shown excellent surgical results for patients with lumbar spinal canal stenosis. However, no reports have directly compared MEL with open laminectomy. This study aimed to elucidate patient-reported outcomes (PROs) and perioperative complications in patients undergoing MEL versus open laminectomy.MethodsThis is a multicenter retrospective cohort study of prospectively registered patients who underwent lumbar spinal surgery at one of the six high-volume spine centers between April 2017 and September 2018. A total of 258 patients who underwent single posterior lumbar decompression at L4/L5 were enrolled in the study. With regard to demographic data, we prospectively used chart sheets to evaluate the diagnosis, operative procedure, operation time, estimated blood loss, and complications. PROs included a numerical rating scale (NRS) for lower back pain and leg pain, the Oswestry Disability Index (ODI), EuroQol 5 Dimension (EQ-5D), and patient satisfaction with the treatment. ResultsOf the 258 patients enrolled, 252 (97%) completed the 1-year follow-up. Of the 252, 130 underwent MEL (MEL group) and 122 underwent open decompression (open group). The MEL group required a significantly shorter operating time and sustained lesser intraoperative blood loss compared with the open group. The overall complication rate was similar (8.2% in the MEL group versus 7.7% in the open group), and the revision rate did not significantly differ. As for PROs, both preoperative and postoperative values did not significantly differ between the two groups. However, the satisfaction rate was higher in the MEL group (74%) than in the open group (53%) (p = 0.02). ConclusionsMEL required a significantly shorter operating time and resulted in lesser intraoperative blood loss compared with laminectomy. Postoperative PROs and complication rates were not significantly different between the procedures, although MEL demonstrated a better satisfaction rate.
The anti-integrity activity of eugenol on inflammatory markers of chronic atherosclerosis
Authors: Karuppasamy Venkadeswaran , Kandhasamy Subramanian , Ramasamy Rajeswari , Sekar Suresh Kumar , Sriniva Venkatesh , Varatharajan varalakshmi , Ramar Suganya , R Subashini , Kannan Shalini 
Published: November 2021

Abstract
The putative anti-atherogenic, anti-oxidative, and anti-inflammatory potential of atherogenic diet-fed, eugenol-treated Wistar rats were studied. The levels of hematological parameters (ESR, WBC, and platelet count); the mean levels of nitric oxide (NO), and C-reactive protein (CRP) were investigated in serum samples. The mean ESR, platelet, and total WBC counts were all significantly higher in atherogenic diet-fed (Group II) rat when compared with normal (Group I), whereas the mean ESR, platelet, and total WBC counts were significantly lower in atherogenic diet-fed followed by eugenol treatment (Group III). The mean hepatic tissue mRNA transcript level of the gene responsible for CRP, iNOS, TNF-α, IL-1β, and NF-κB and expression of their protein levels were determined in liver tissue samples were found to be significantly higher in the atherogenic diet-fed followed saline treatment (Group II) rats than the normal fed (Group I) rats. However, in hepatic tissue samples from an atherogenic diet-fed followed by eugenol treated rats, the mean mRNA the transcript level of the gene encoding CRP, iNOS, TNF-α, IL-1β, NF-κB and their mean concentration of the protein translation were significantly lower than the values in Group II rat’s hepatic tissue, approaching near-normal levels. These results are exhibited anti-atherogenic potential in hypercholesterolemic Wistar rats due to their hypocholesterolemic, antioxidant, and anti-inflammatory effects.