bluuebunny/crossref_metadata_2025_split huggingface dataset:
“Necessarily Hidden Truth(s)”: Documenting Queer Migrant Experience in Rigoberto González’s Crossing Vines
Authors: de la Garza Valenzuela José A 
Published: September 2021

Abstract
The interpretation of documents holds together the center of Rigoberto González’s Crossing Vines (2003). Presented as time-stamped vignettes detailing the life of laborers at a vineyard for an university assignment, the novel throws into question the distance between the experiences the characters have and those accessible to Leonardo, the ethnographer-in-training. In Crossing Vines, queer migrant characters such as Aníbal and Moreno develop a narrative agency over access to details that the government or ethnographer might solicit in these narrative gaps. Analyzing González’s use of character’s institutional and personal archives, such as Permanent Resident Cards/green cards, photographs, and Leonardo’s ethnography, I argue that despite offering competing narratives about migrant life, these documents methodologically limit how queer migrants are textually represented and interpreted. Crossing Vines critiques the institutional processes by which migrant stories are gathered, interpreted, and violently cross-examined to foreground the blurred lines between studying and surveilling migrant communities. Whereas Leonardo’s project and the inspection of green cards puts pressure on the migrants in the novel to disclose information to produce or cross-reference an institutional archive, González asks us to put the limitations of the documents under scrutiny. In representing what these archives cannot, Crossing Vines makes a case for the utility and centrality of fiction in attending to the often intentional oversights of institutional archives, including those in our own fields of study.
Turbulent transport of radiation in the solar convective zone
Authors: Rogachevskii I , Kleeorin N 
Published: September 2021

ABSTRACTA turbulent transport of radiation in the solar convective zone is investigated. The mean-field equation for the irradiation intensity is derived. It is shown that due to the turbulent effects, the effective penetration length of radiation can be increased several times in comparison with the mean penetration length of radiation (defined as an inverse mean absorption coefficient). Using the model of the solar convective zone based on mixing length theory, where the mean penetration length of radiation is usually much smaller than the turbulent correlation length, it is demonstrated that the ratio of the effective penetration length to the mean penetration length of radiation increases 2.5 times in the vicinity of the solar surface. The main reasons for this are the compressibility effects that become important in the vicinity of the solar surface where temperature and density fluctuations increase towards the solar surface, enhancing fluctuations of the radiation absorption coefficient and increasing the effective penetration length of radiation.
Uterine kisspeptin receptor critically regulates epithelial estrogen receptor α transcriptional activity at the time of embryo implantation in a mouse model
Authors: Schaefer Jennifer , Vilos Angelos G , Vilos George A , Bhattacharya Moshmi , Babwah Andy V 
Published: September 2021

Abstract
Embryo implantation failure is a major cause of infertility in women of reproductive age and a better understanding of uterine factors that regulate implantation is required for developing effective treatments for female infertility. This study investigated the role of the uterine kisspeptin receptor (KISS1R) in the molecular regulation of implantation in a mouse model. To conduct this study, a conditional uterine knockout (KO) of Kiss1r was created using the Pgr-Cre (progesterone receptor-CRE recombinase) driver. Reproductive profiling revealed that while KO females exhibited normal ovarian function and mated successfully to stud males, they exhibited significantly fewer implantation sites, reduced litter size and increased neonatal mortality demonstrating that uterine KISS1R is required for embryo implantation and a healthy pregnancy. Strikingly, in the uterus of Kiss1r KO mice on day 4 (D4) of pregnancy, the day of embryo implantation, KO females exhibited aberrantly elevated epithelial ERα (estrogen receptor α) transcriptional activity. This led to the temporal misexpression of several epithelial genes [Cftr (Cystic fibrosis transmembrane conductance regulator), Aqp5 (aquaporin 5), Aqp8 (aquaporin 8) and Cldn7 (claudin 7)] that mediate luminal fluid secretion and luminal opening. As a result, on D4 of pregnancy, the lumen remained open disrupting the final acquisition of endometrial receptivity and likely accounting for the reduction in implantation events. Our data clearly show that uterine KISS1R negatively regulates ERα signaling at the time of implantation, in part by inhibiting ERα overexpression and preventing detrimentally high ERα activity. To date, there are no reports on the regulation of ERα by KISS1R; therefore, this study has uncovered an important and powerful regulator of uterine ERα during early pregnancy.
TARG1 protects against toxic DNA ADP-ribosylation
Authors: Tromans-Coia Callum , Sanchi Andrea , Moeller Giuliana K , Timinszky Gyula , Lopes Massimo , Ahel Ivan 
Published: September 2021

Abstract
ADP-ribosylation is a modification that targets a variety of macromolecules and regulates a diverse array of important cellular processes. ADP-ribosylation is catalysed by ADP-ribosyltransferases and reversed by ADP-ribosylhydrolases. Recently, an ADP-ribosyltransferase toxin termed ‘DarT’ from bacteria, which is distantly related to human PARPs, was shown to modify thymidine in single-stranded DNA in a sequence specific manner. The antitoxin of DarT is the macrodomain containing ADP-ribosylhydrolase DarG, which shares striking structural homology with the human ADP-ribosylhydrolase TARG1. Here, we show that TARG1, like DarG, can reverse thymidine-linked DNA ADP-ribosylation. We find that TARG1-deficient human cells are extremely sensitive to DNA ADP-ribosylation. Furthermore, we also demonstrate the first detection of reversible ADP-ribosylation on genomic DNA in vivo from human cells. Collectively, our results elucidate the impact of DNA ADP-ribosylation in human cells and provides a molecular toolkit for future studies into this largely unknown facet of ADP-ribosylation.
ProNAB: database for binding affinities of protein–nucleic acid complexes and their mutants
Authors: Harini Kannan , Srivastava Ambuj , Kulandaisamy Arulsamy , Gromiha M Michael 
Published: September 2021

AbstractProtein–nucleic acid interactions are involved in various biological processes such as gene expression, replication, transcription, translation and packaging. The binding affinities of protein–DNA and protein–RNA complexes are important for elucidating the mechanism of protein–nucleic acid recognition. Although experimental data on binding affinity are reported abundantly in the literature, no well-curated database is currently available for protein–nucleic acid binding affinity. We have developed a database, ProNAB, which contains more than 20 000 experimental data for the binding affinities of protein–DNA and protein–RNA complexes. Each entry provides comprehensive information on sequence and structural features of a protein, nucleic acid and its complex, experimental conditions, thermodynamic parameters such as dissociation constant (Kd), binding free energy (ΔG) and change in binding free energy upon mutation (ΔΔG), and literature information. ProNAB is cross-linked with GenBank, UniProt, PDB, ProThermDB, PROSITE, DisProt and Pubmed. It provides a user-friendly web interface with options for search, display, sorting, visualization, download and upload the data. ProNAB is freely available at https://web.iitm.ac.in/bioinfo2/pronab/ and it has potential applications such as understanding the factors influencing the affinity, development of prediction tools, binding affinity change upon mutation and design complexes with the desired affinity.
JS01.4.A The neurocognitive function changes with awake craniotomy for low-grade glioma in the left hemispheric eloquent regions
Authors: Wang Y , Ji P , Guo S , Liu J , Zhai Y , Wang N , Qu Y , Wang L 
Published: September 2021

Abstract

BACKGROUND
Cognitive deficit was frequently observed in glioma patients, especially for those on the eloquent area. Considering the increased life expectancy, brain mapping during awake craniotomy was preferentially applied to exacerbate neurocognitive deficits. The aim of the current study was to evaluate the neurocognitive changes during the perioperative period of resection of low-grade glioma (LGG) in the left side eloquent area with awake craniotomy in a major neurosurgical center in China for 5 years.

MATERIAL AND METHODS
We retrospectively analyzed patients with left-sided glioma in eloquent areas, who received awake craniotomy during 2016–2020. Montreal Cognitive Assessment Scale, BN-20, and EORTC-QLQ-C30 questionnaire were applied for neurological cognitive assessment. We performed a correlation analysis between changes in cognitive performance and tumor characteristics, including tumor location, pathological grade. Treatment-related factors were also analyzed, such as the extent of resection (EOR), preoperative and postoperative Karnofsky Performance Score (KPS), postoperative treatment strategy (chemo- and radiotherapy), progression-free survival (PFS), overall survival (OS).

RESULTS
68 patients were included in our current study. For the language domain, memory domain, and executive functions, 7.4% (5/68) patients presented mild postoperative cognitive performance deterioration compared to preoperative. Tumor location was the only factor that greatly influenced the postoperative cognitive performance, while other features (EOR, KPS, pathological grades) and treatment strategy were found no effect on cognitive change. The extent of tumor resection ranged from 81% to 100%.

CONCLUSION
Our study underlines the importance of the application of brain mapping during awake craniotomy, which helps to maximize extent of tumor resection while preserving cognitive function in individuals with LGG in eloquent regions.
OS03.4.A Irradiation of the subventricular and subgranular zone and overall survival in high-grade glioma patients
Authors: Bruil D , David S , Nagtegaal S , Verhoeff J 
Published: September 2021

Abstract

BACKGROUND
Previous research has shown that neural stem cells (NSCs) in the subventricular zone (SVZ) may support the growth of glioma by recruiting new cells to the tumor. NSCs are located in the SVZ as well as in the subgranular zone (SGZ) of the hippocampus, the two neurogenic niches of the brain. This might indicate that irradiation of the SVZ and SGZ, and thereby damaging NSCs, reduces tumor growth and improves overall survival (OS). However, irradiation may also inhibit the repair capacity of healthy brain tissue by these neurogenic niches. Therefore, we investigated the effects of SVZ and SGZ irradiation dose on OS, in a cohort of high-grade glioma patients.

MATERIAL AND METHODS
We have retrospectively selected 221 patients (2014–2020) with WHO grade III and IV gliomas that underwent radiotherapy. Next to clinical baseline characteristics, T1 weighted MRI- and CT-images were collected. The SVZ and SGZ regions on the individual T1 images were delineated via non-linear registration of brain atlases. SVZ labels were created in 0.5mm isotropic MNI T1 and T2 templates, while SGZ atlas labels were available via the Hippocampus and Subfields CoBrA atlas. Next, the mean dose from the acquired SVZ and SGZ labels were extracted. The relationship between SVZ doses, SGZ doses and OS were examined using the Cox proportional hazards model and the Kaplan-Meier method (using the Log Rank test for significance).

RESULTS
For the mean dose in the SVZ, the hazard ratio (HR) was 1.024 per Gy (P = 0.002, [95% confidence interval, 1.009–1.040]) and the mean SGZ dose had a HR of 1.021 per Gy (P&lt; 0.001, [95% confidence interval, 1.012–1.031]). These results were then corrected for the following covariates: sex, age, total intracranial volume and extent of surgery. This resulted in a HR of 1.031 per Gy (P = 0,001, [95% confidence interval, 1.014–1.050]) for the mean SVZ dose, and a HR of 1.025 per Gy (P&lt; 0.001, [95% confidence interval, 1.015–1.036]) for the mean SGZ dose. Patients whose SVZ received greater than the median SVZ dose (= 31.3 Gy) showed a significant decrease in OS compared to patients who received less than the median dose (10.7 months vs 13.5 months median OS, P = 0.001). Patients whose SGZ received greater than the median SGZ dose (= 31.9) showed a significant decrease in OS compared to patients who received less than the median dose (10.7 months vs 15.1 months median OS, P&lt; 0.001).

CONCLUSION
Here, we present a large cohort of high-grade glioma patients, in which we show a statistically significant decrease in overall survival with increasing radiation dose on the SGZ and SVZ. This correlation suggests that both neurogenic niches might need to be spared during radiotherapy treatment to improve overall survival even in high-grade glioma patients. Modern radiotherapy planning and delivery options are available to implement this.
OS06.5A Fluorescence image-guided resection of intracranial meningioma: an experimental in vivo study on nude mice
Authors: Linsler S , Senger S , Müller S , Müller A , Oertel J 
Published: September 2021

Abstract

BACKGROUND
The use of photodynamic agents in malignant cranial tumor surgery is quite common. For example five-aminolevulinic acid (5-ALA)-induced porphyrins in malignant gliomas are potent photosensitizers. Until today there is no comparable selective fluorescent substance available for meningiomas. Nevertheless, there is a demand for intraoperative fluorescent identification of e.g. invasive skull base meningiomas to increase radicality. This study was established to investigate fluorescent image-guided resection with somatostatin receptor labelled fluorescence dye for intracranial meningioma in the nude mice.

MATERIAL AND METHODS
Primary meningioma cell culture samples were stereotactically implanted subdural into 20 nude mice. 90 days after inoculation of the cells, a cranial MRI with contrast agent revealed tumor growth. After detection of tumor mass in MRI, FAM-TOC5,6-Carboxyfluoresceine-Tyr3-Octreotide was injected intravenously and tumor mass was hereafter resected under visualization via fluorescence microscope and endoscope. After attempted total resection, animal were sacrificed brain slices were obtained and histologically analysed to verify the resection extent.

RESULTS
In 18 mice tumor growth was detected in MRI after 90 days of inoculation. The tumor mass could be clearly identified with fluorescence microscope and endoscope after injecting FAM-TOC5,6-Carboxyfluoresceine-Tyr3-Octreotide. The tumor margins could be better visualized. After fluorescence-guided resection no remaining tumor could be identified in histological analysis.

CONCLUSION
This study describes for the first time the use of FAM-TOC5,6-Carboxyfluoresceine-Tyr3-Octreotide and demonstrates its value of fluorescent identification of meningioma cells in vivo. Furthermore, the authors established a new experimental animal model for fluorescence meningioma surgery.
P11.01 Symptom networks in glioma: a novel approach to study multidimensional symptomatology in glioma patients
Authors: Varkevisser T M C K , Röttgering J G , de Witt Hamer P , Kouwenhoven M C M , Reijneveld J C , Belgers V , van Lingen M , Klein M , Douw L 
Published: September 2021

Abstract

BACKGROUND
Glioma patients experience a high symptom burden contributing to poorer quality of life. Symptoms include depression, cognitive impairment, and fatigue and vary throughout the disease. These symptoms are rarely studied from a comprehensive perspective, while their interdependence may be relevant for their development, perpetuation, and ultimately successful treatment. The emerging field of symptom network analysis uncovers the multidimensional symptom space. Nodes are the symptoms, and edges are operationalized as the full conditional association, or partial correlation, between two symptom severity scores across patients. Highly connected nodes are considered central and may be particularly relevant targets for treatment as disruption of these central nodes impact the entire network. We visualized the overall glioma symptom network, compared multidimensional results to known literature, and statistically compared networks between relevant patient subgroups.

MATERIAL AND METHODS
A dataset comprised of 355 observations of 180 glioma patients at different disease phases was analysed. Cognitive testing and questionnaires regarding health-related and glioma-specific quality of life, fatigue, depression, and cognition resulted in the definition of 30 symptom nodes. Symptom clusters were visually explored in the resulting networks, as were node strength, betweenness, and closeness centrality measures for each node. Networks were statistically compared between preoperative patients and patients during stable disease, as well as patients with low versus high-grade gliomas. Networks of patients with normal versus severe levels of fatigue were also compared as cancer-/glioma-related fatigue has a strong impact on quality of life and can correlate with other common symptoms such as pain, depression, and/or sleep disturbance.

RESULTS
Symptom clusters existed between: 1) bodily pain, headache and physical functioning; 2) concentration and motivation; and 3) fatigue and drowsiness. Fatigue and mental health were the most central nodes in the networks. Furthermore, the overall connectivity between symptoms was significantly higher in the severely fatigued patients than in patients with normal fatigue. No network differences were found between low versus high-grade, and preoperative versus stable disease networks.

CONCLUSION
Fatigue is a central node in glioma patients’ burden of disease, and symptoms are more tightly intercorrelated in patients experiencing severe fatigue. From our data, we hypothesize that fatigue co-exists with or perpetuates other symptoms. Thus, although these results are preliminary, the network approach may innovate hypothesis generation in symptom management.
P11.04 Autonomy duration as analyzed by KPS≥70 cumulative time in patients with biopsy-only glioblastoma (BO-GBM). A sub-analysis of the Timone cohort
Authors: Harlay V , Loundou A , Boucard C , Petrirena G , Barrie M , Campello C , Autran D , Padovani L , Figarella D , Boissonneau S , Dufour H , Baumstarck K , Tabouret E , Chinot O 
Published: September 2021

Abstract

BACKGROUND
Improvement or maintenance of autonomy is a crucial and understudied issue for glioblastoma (GBM) patients whose outcome is poor. Biopsy-only GBM (BO-GBM) is a situation where survival is short and independence is of particular importance. Our objective was to explore functional outcome in biopsy-only patients.

MATERIAL AND METHODS
A regional glioma SIRIC cohort was conducted at CHU Timone in 2014–2017 and we retrospectively reviewed the BO-GBM subgroup. We prospectively collected age, corticosteroid dose, tumoral surface, treatment allocated and completed, and survival outcome. Functional independence was analyzed as a cumulative time of Karnofsky performance status (KPS) ≥70 from the date of diagnosis until death. We analyzed potential factors associated to time with KPS ≥70.

RESULTS
Among 535 patients enrolled in the cohort, surgery was restricted to biopsy in 139 patients (BO-GBM). Mean tumoral surface measured on gadolinium-enhanced T1-weighted MRI was 1198mm2 (min: 65; max: 4515mm2). Mean steroid dose at diagnosis was 50mg prednisolone per day. Corticosteroid dose was ≥50mg prednisolone per day for 77 patients and &lt;50mg per day for 56 patients. Fifty-four patients (39%) were referred to radiotherapy-temozolomide (RT-TMZ), 68 (49%) considered unfitted for RT received chemotherapy upfront only (CT-UF), and 17 patients (12%) were referred to palliative care only. Median overall survival (OS) was 7.5 months (95%CI: 6.0–9.2), 14.0 months (95%CI: 9.7–18.7) and 6.0 months (95%CI: 4.6–7.7) for BO-GBM, RT-TMZ and CT-UF respectively. At diagnosis, 81 (58.3%) patients presented with self-care capacity (KPS ≥ 70%). For these patients, median time of autonomy preservation was 7.6 months (95%CI: 6.1–9.0). Median time of autonomy preservation differed according to treatment modalities: it was 8.6 months (95%CI: 5.9–11.3) versus 6.3 months (95%CI: 2.9–9.7) for RT-TMZ versus CT-UF group respectively (p&lt;0.001). In univariate analysis, time with KPS ≥ 70% was correlated with age (p=0.001), initial KPS (p&lt;0.001), tumoral surface measured on gadolinium-enhanced T1-weighted MRI (p=0.03) and corticosteroid dose (p=0.001). In multivariate analysis, time with KPS≥70 was correlated with age (p=0.001) and KPS at diagnosis (p&lt;0.001).

CONCLUSION
Patients with inoperable GBM referred to radiotherapy-temozolomide present a valuable duration of functional independence, although shorter in patients not referred to RT. Duration of functional independence could be considered in addition to PFS and OS for treatment evaluation in patients with GBM.
P13.15 Pre-clinical trial of T601 oncolytic virus for high grade glima via intra-tumoral injection
Authors: He Z , Meng Z , Liang P , Xing L , Zheng X , Wang G 
Published: September 2021

Abstract

BACKGROUND
An effective therapeutic method still hasn’t been devised for lethal high grade glioma. Thus, a method with high anti-tumoral efficiency, tumoral targeting, and acceptable side effect needs to be designed. Oncolytic virotherapy which can specifically lyse tumor cells via mass replication and deleting nucleotide metabolism related gene, like TK, required in viral replication and overexpressed in tumor cells, provides hope for patients. However, the virus only contained TK deletion is unable to show sufficient specificity of anti-tumoral response in tumor cells. Here, the adapted strain of vaccinia virus with high tumoral specificity due to TK and RR deletion and FUC1 insertion, named T601, is chosen in this project. In clinical application, intra-tumoral injection showed improved anti-tumoral efficiency and acceptable side effect. However, intra-tumoral viral injection in orthotropic glioma model is rare. In this project, various biosafety and antitumoral efficiency parameter would be tested for confirming the biosafety and reliability of intra-tumoral T601 viral injection for future clinical trials.

MATERIAL AND METHODS
For measuring the IC50 of T601, 10 different amounts of virus was tested in vitro via calculating cell viability with CCK-8(cell counting kit-8). For measuring the further antitumoral response of FCU1, different concentration of the 5-FC was added into the medium with IC50 viral amount. To ensure the biosafety of T601, MTD (maximum tolerance dose) was measured. Based on the MTD result, for evaluating the anti-tumoral efficiency, 106 pfu,105 pfu,104 pfu of virus was intra-tumoral injected in orthotopic GBM bearing mice. Tumor size was measured once a week through in vivo bioimaging system.

RESULTS
0.022 MOI, the IC50 of T601, showed high cytotoxicity of T601. Moreover, the significantly decreased cell viability under the combined treatment of 5-FC and 0.22MOI T601 showed intact anti-tumoral function. In MTD assay, except for 107 group, no significant weight loss was found. However, in 107 pfu group, mean body weight decreased around 10% and animal fatality happened on day 9. According to the MTD result, certain amount of virus was intra-tumorally injected. In all treatment group, the tumor size was significantly shrined. At the same time, the survival rate of mice under viral treatment was significantly extended.

CONCLUSION
In summary, T601 exhibited efficient anti-tumoral function and acceptable side effect. T601 treatment prolonged the survival period of GBM mice with acceptable neurotoxicity, demonstrating that T601 contains necessary criterial for intra-tumoral injection. Ultimately, this project provided basic reference information of dose for future clinical trial.