bluuebunny/crossref_metadata_2025_split huggingface dataset:
Relation of Hypertension With Migraine and Chronic Daily Headache in Population of Rafsanjan Cohort Study
Authors: Mohammadi Movahedeh , Ayoobi Fatemeh , Khalili Parvin , Soltani Narjes , Vecchia Carlo La , Vakilian Alireza 
Published: February 2021

Abstract
Background: Headache has a variety of types, such as Migraine and chronic daily headache (CDH) in its primary form. There is a positive correlation between these two types of headaches and hypertension (HTN), but in some works this correlation has been reported negatively. Therefore, we planned to study HTN-Migraine as well as HTN-CDH correlation in our population.Methods: A sample of Rafsanjan population (10000 individuals) entered the cohort study, as a part of the prospective epidemiological research studies in IrAN (PERSIAN). In this population, we compared the frequency of HTN categories in cases with Migraine and CDH with a normal population (without Migraine).Results: Out of 9990 participants (46.6% males and 53.4% females) about 29% had Migraine and 7.5 % had CDH.HTN was found in 24.27% of Migraine and 31.98% of CDH cases. HTN was also found to be associated with Migraine and CDH in the crude model. Two Categories of HTN (Long controlled and uncontrolled) were not associated with Migraine. On the other hand, CDH showed associations with all the HTN categories. Moreover, in a model, which included all variables and confounders, Migraine and CDH had association with HTN without any considerable changes.Conclusion: Our study showed that there is strong HTN-Migraine as well as HTN-CDH correlations in the studied population.
Effects of Prophylactic Antibiotic Administration and Antibiotic Timing on Culture Results and Clinical Outcomes of Pediatric Musculoskeletal Infection: A Protocol for A Randomized, Controlled, Clinical Trial
Authors: Xia Yongjie , Deng Chao , Zhou Yibiao , Wu Dechao , Liu Zhiyong , Xie Liangfu , E Bing , Han Jingming , You Chao 
Published: February 2021

Abstract
Background: Musculoskeletal infection (MSI) is a common cause of morbidity among the pediatric population. Some clinicians recommend withholding prophylactic antibiotics until culture collection with an aim to improve the culture sensitivity. However, a recent retrospective study reported that prophylactic antibiotic administration did not affect culture sensitivities in either disseminated or local MSI in pediatric population, which is surprising. Therefore, the aim of this study is to investigate the effects of prophylactic antibiotic administration and the timing of antibiotic administration on culture sensitivity and clinical outcomes of pediatric MSI.Methods: A randomized, controlled, clinical trial will be carried out. Individuals aged 0 to 18 years with a diagnosis of MSI will be screened and evaluated at the Shenzhen Children’s Hospital. The participants will be randomly allocated into four groups, and they will receive the antibiotic treatment at different time points, that is, 1 week, 3 days, 1 day prior to culture collection and 1 day after culture collection, respectively. The primary outcome will be culture sensitivity. In addition, the disease-related markers including white blood cell count (WBC), C-reactive protein (CRP), erythrocyte sedimentation rate, vital signs as well as the length of hospital stay will be measured or recorded accordingly. Using chi-squared tests, the rates of positive cultures will be compared between different groups. Statistical comparisons between the different patient groups regarding the confounding and outcome variables will be conducted using independent t-tests, Mann-Whitney U tests, chi-squared tests and Fisher’s exact tests as appropriate with the significance level set to 5% (P<0.05).Discussion: The results of this study would provide some evidence for the clinical management of pediatric MSIs with regards to the application of prophylactic antibiotics.Trial registration: Chinese Clinical Trial Registry, ChiCTR2100041631. Registered at 1 January 2021, http://www.chictr.org.cn/hvshowproject.aspx?id=69850.
Mitogenomic Diversity and Maternal Origins of Yak (Bos Grunniens)
Authors: Wang Xingdong , Pei Jie , Bao Pengjia , Cao Mengli , Guo Shaoke , Song Rende , Song Weiru , Liang Chunnian , Yan Ping , Guo Xian 
Published: February 2021

Abstract
Background and aim: Mitochondrial genome has the characteristics of simple structure, no recombination, maternal inheritance, high conservation, fast evolution, and high copy number. It is easy to sequence, contains high resolution phylogenetic information, and exists in a wide range of taxa. Theref ore, it is widely used in the study of biological phylogeny. At present, phylogenetic studies focus mainly on D loop region, cytochrome b gene, and protein coding sequence; phylogenetic studies using the mitochondrial complete sequence are rare. Therefore, this study aimed to conduct phylogenetic experiments using the mitochondrial complete sequence and compare the results with previous findings obtained using partial sequences. Results: Complete mitochondrial sequences of five yak populations from Qinghai and Xinjiang were obtained. The haplotype diversity of the five populations were Xueduo yak (0.992 ± 0.015), Pamir yak (0.990 ± 0.014), Yushu yak (0.963 ± 0.033), Qilian yak (0.948 ± 0.036), and Huanhu yak (0.905 ± 0.048), which showed a higher haplotype diversity compared with other breeds. A total of 78 haplotypes were obtained from 111 individuals. Among these, Yushu yak, Huanhu yak, Xueduo yak, and Qilian yak all shared haplotypes, but the Pamir yak did not share haplotypes with these four populations. Phylogenetic analysis showed that yak populations were separable into three distinct branches. The analysis identified a new phylogenetic branch with both wild and domestic yaks being represented in the new branch. The 155 haplotypes were divided into 6 haplotype groups by haplotype clustering. The haplotype group had nothing to do with the morphological group of yaks, and yaks from the same population or the same ecological group were distributed in different haplotype groups. The four haplotype groups A, B, C, and D, showed a star-shaped distribution of haplotypes. The central haplotypes were widely distributed and had a high frequency Conclusions: T he genetic diversity of yaks in Qinghai was high. Both domestic and wild yaks exhibited three different mat ernal origins. Three different maternal origins existed in ancient wild yaks before domestication, with haplogroup A being the primary haplogroup associated with yak domestication.
Electrolysis-Driven Bioremediation to Enhance Nitrogen and Phosphorus Removal From Polluted River Sediment
Authors: Li Sanshan , Ma Tangming , Zheng Chaoqun , He Zhaofang , Yang Liuyan , Gao Yan 
Published: February 2021

Abstract
In order to testify the effect of electrolysis and microbial remediation technology in polluted river sediment. Here, we explored the possibility of electrochemically removing ammoniacal nitrogen-nitrogen (NH3-N), nitrate-nitrogen (NO3−-N) and phosphate ions-phosphorous (PO43−-P) by using a titanium (Ti) mesh cathode, a Ti/Ti dioxide (TiO2)/Ruthenium (IV) oxide (RuO2) (RuO2-IrO2/Ti) mesh, and a magnesium-aluminum (Mg–Al) alloy anode placed within the sediment and overlying water. Results showed that approximately 151.82 ± 21.69 mg TN was removed which was five times more effective than the non-electrolytic controls (30.21 ± 13.73 mg), NH3-N concentration in the sediment was substantially reduced (up to 2.9 times) compared to the non-electrolytic controls. Its efficiency lies in the electrolysis process, which may directly remove NH3-N through electrochemical oxidation and simultaneously produce oxygen which helps nitrifying bacteria to convert NH3-N into NO3−-N by the role of anode; and electrolysis may directly remove NO3−-N in the overlying water through electrochemical reduction while simultaneously producing hydrogen electron donor for hydrogen autotrophic microorganism as Hydrogenophohaga, to be the dominant species in sediment to enhance the removal of NO3−-N by the role of cathode. Electrolysis also reduced the PO43−-P through electro-coagulation since Mg2+ ions could also produce since sacrificial Mg–Al alloy anode was used and electro-deposition on Ti mesh cathode both to increase PO43−-P removal in overlying water and sediment. This study verifies the benefits of electrolysis-driven bioremediation as a sustainable technology for the bioremediation of N and P polluted river sediments.
Moods and Ethics of Justice
Authors: Pecujlija Mladen , Kokotovic Sonja , Miletic Ljiljana 
Published: February 2021

Abstract
In 2019, a survey was conducted with 2593 undergraduate students to investigate relation among their ethical orientation toward justice and moods. The research was conducted by means of these two instruments: by ethical orientation questionnaire and by PANAS X scale, instrument that is based on Watson’s model of affective experience. The results indicate that basic positive affect (BPA) and basic negative affect (BNA) are able to predict ethical orientation toward justice.
Combined Coronary CT&nbsp;Angiography With Plain Scan for Diagnosis of Ruptured Plaque: Comparison With Optical Coherence Tomography&nbsp;
Authors: Wang Zhao-qian , Zhang Hai-xia , Wu Wei , Yuan You-sheng , Dou Ya-na , Yin Da , Li Xin-sheng , JIA Chong-fu 
Published: February 2021

Abstract
Purpose: To explore the diagnostic efficacy of CCTA + plain scan for ruptured plaques with optical coherence tomography (OCT) as the reference, and to preliminarily analyze the influential factors. Methods: Patients who underwent CCTA and OCT were retrospectively enrolled. Signs of ruptured plaque (plaque ulcer or intra-plaque dye penetration) and other plaque measurements on CCTA were recorded and compared with plain scans (calcification scoring scan) to differentiate calcifications from rupture signs. By OCT, presence of ruptured plaque was determined. Total 65 patients with 71 plaques were included. Results: There were 40 OCT-confirmed ruptured plaques in 38 patients and 31 OCT-confirmed non-ruptured plaques in 27 patients. CCTA+ plain scan identified 27 ruptured plaques in 27 patients and 28 non-ruptured plaques in 24 patients. With OCT as the gold standard, the per-patient sensitivity, specificity, positive and negative predictive values, and accuracy of CCTA + plain scan for diagnosing ruptured plaque were 71%, 89%, 90%, 69%, and 78%, and there was good agreement (Kappa=0.70) between CCTA + plain scan and OCT. Among 13 false negative ruptured plaques, 2 had calcifications close to the rupture, and the cavity depth in the remaining 11 was 0.46 ±0.17 mm, vs. 0.98±0.26 mm in 27 true positive ruptured plaques (P<0.01).Conclusion: CCTA + plain scan has high specificity and positive predictive value in diagnosing ruptured plaque with medium sensitivity and negative predictive value. The cavity depth of ruptured plaques and calcifications at the rupture site may be the main influential factors.
Ultra-sonication enhanced green synthesis of silver nanoparticles using Barleria buxifolia leaf extract and its possible application
Authors: sekar Vanaraj 
Published: February 2021

Abstract
A simple and eco-friendly method for the green synthesis of silver nanoparticles (AgNPs) by ultrasound-assisted strategy using Barleria buxifolia leaf extract as a reducing and capping agent was established in this study. The obtained AgNPs were characterized. UV-vis spectrum, Fourier transform infrared spectroscopy (FTIR), scanning and transmission electron microscopy (SEM and TEM), Energy Dispersive X-Ray Analyzer (EDX), X-ray diffraction, dynamic light scattering (DLS) analysis showed that the obtained AgNPs were mono dispersed spheres with uniform size of 80 nm. UV-vis spectroscopy, FTIR, and XRD analysis indicated that the surface of the obtained AgNPs was covered with organic molecules in plant extracts. The results of ABTS assays showed that high antioxidant activity was seen in the obtained AgNPs. Green synthesized AgNPs showed potent antibacterial and anti-biofilm activity against tested pathogens. Cytotoxicity assay showed that the obtained AgNPs were significantly cytotoxic to cancer cell line (MCF-7). In addition, the AgNPs synthesized in this paper can also photo catalytically degrade methylene blue dye under visible light. The potent bioactivity exhibited by the green synthesized silver nanoparticles leads towards the multiple use as antioxidant, antibacterial, anti-biofilm, cytotoxic as well as photo catalytic agent.
Numerical Simulation of the Combined Slope Protection Effect of Live Stump and Bamboo Anchor
Authors: Hui Yang , Zhu Yingbo , Liu Yong , Jiang xueliang , Deng Riwen , Huang Li , yin Peng , Lai Guojie 
Published: February 2021

Abstract
Five slope states were selected to research deep protection effect of the living tree stump-bamboo anchor supporting structure. These five states were the natural slope state,the bamboo anchor slope protection state, and the living tree stump-bamboo anchor initial supporting state,living tree stumps-bamboo bolt mid-term support status,living tree stump slope protection status. Three-dimensional numerical models are established by Midas GTS/NX finite element software, and the force characteristics and stability of the living tree stump-bamboo anchor support structure were studied. The results show that: (1) Compared with natural slopes, the relative stability and safety factors of bamboo anchor slope protection, live tree stumps-bamboo anchor initial slope protection, live tree stumps-bamboo anchor mid-term slope protection, and live tree stump slope protection have increased by 18.6%, 19.7%, and 44.0 %, 44.1%, it can be found that the living tree stump-bamboo bolt supporting structure has obvious deep protection effect. (2) In the initial supporting state of live tree stumps and bamboo anchors, the bamboo anchors have the largest stress value at the position where the bamboo anchors are buried at a depth of 5m on the slope; The bamboo anchors in the fourth row and the fifth row are below the buried depth of 6m on the slope, and the stress value of the bamboo anchors increases linearly. (3) The closer to the toe of the slope, the greater the tensile stress generated by the root system on the left side of the living tree stump, and the greater the compressive stress generated by the root system on the right side of the living tree stump. The research results can be used as an important basis for the new living tree stump-bamboo bolt support structure to effectively prevent and control deep-seated slope protection, and it is of great significance to expand the green technology for preventing and controlling deep-seated landslides.
Significance of m 6A Methyltransferase‑Related lncRNAs in the Prognosis and Immunotherapy of Hepatocellular Carcinoma
Authors: Li Lili , Xie Rongrong , Wei Qichun 
Published: February 2021

Abstract
Background: Hepatocellular carcinoma (HCC) is one of the leading causes of mortality worldwide. N6-methyladenosine (m6A) methyltransferase, has been proved to act as an oncogene in several human cancers. However, little is known about its relationship with the long non-coding RNAs (lncRNAs) that remains elusive in HCC.Methods: We comprehensively integrated gene expression data acquired from 371 HCC and 50 normal tissues in The Cancer Genome Atlas (TCGA) database. Differentially expressed protein-coding genes (DE-PCGs)/lncRNAs (DE-lncRs) analysis and univariate regression & Kaplan-Meier (K-M) analysis was performed to identify m6A methyltransferase‑related lncRNAs that were related to overall survival (OS). m6A methyltransferase‑related lncRNA signature was constructed using the Least absolute shrinkage and selection operator (LASSO) Cox regression analyses. Furthermore, Cox regression analysis was applied to identify independent prognostic factors in HCC. The signature was validated in an internal validation set. Finally, the correlation analysis between gene signature and immune cells infiltration was also investigated via single-sample Gene Set Enrichment Analysis (ssGSEA) and immunotherapy response was calculated through Tumor Immune Dysfunction and Exclusion (TIDE) algorithm.Results: A total of 21 m6A methyltransferase-related lncRNAs were screened out according to Spearman correlation analysis with the immune score (|R| > 0.3, P < 0.05). We selected 3 prognostic lncRNAs to construct m6A methyltransferase-related lncRNA signature through univariate and LASSO Cox regression analyses. The univariate and multivariate Cox regression analyses demonstrated that the lncRNAs signature was a robust independent prognostic factor in OS prediction with high accuracy. The GSEA also suggested that the m6A methyltransferase-related lncRNAs were involved in the immune-related biological processes and pathways which were very well-known in the context of HCC tumorigenesis. Besides, we found that the lncRNAs signature was strikingly correlated with the tumor microenvironment (TME) immune cells infiltration and expression of critical immune checkpoints. Finally, results from the TIDE analysis revealed that the m6A methyltransferase-related lncRNAs could efficiently predict the clinical response of immunotherapy in HCC.Conclusion: Together, our study screened potential prognostic m6A methyltransferase related lncRNAs and established a novel m6A methyltransferase-based prognostic model of HCC, which not only provides new potential prognostic biomarkers and therapeutic targets but also deepens our understanding of tumor immune microenvironment status and lays a theoretical foundation for immunotherapy.
CMR-Verified Myocardial Fibrosis is Associated With Subclinical Diastolic Dysfunction in Primary Aldosteronism Patients
Authors: Zhou Fangli , Wu Tao , Wang Wei , Cheng Wei , Wan Shuang , Tian Haoming , Chen Tao , Sun Jiayu , Ren Yan 
Published: February 2021

Abstract
The main cardiac features of primary aldosteronism (PA) are impaired left ventricular (LV) diastolic function, and some articles also reported more cardiac fibrosis in PA patients. However, the correlation between LV dysfunction and diffuse myocardial fibrosis in PA remains unknown. We enrolled 84 PA patients and 28 essential hypertension (EH) patients in West China Hospital. Cardiac magnetic resonance imaging (CMR) contrast enhancement was arranged for all subjects. Postcontrast T1 time and left ventricular myocardial strains and strain rates were measured. 76 PA patients and 27 essential hypertension (EH) patients were included in the final analysis. Blood pressure, LV mass indexes, and LV ejection fractions were comparable in both groups, while the global circumferential peak diastolic strain rate (PDSR) was lower (53 ± 20 vs. 68 ± 25, p<0.01) and the postcontrast T1 time was shorter (520 ± 38 vs. 538 ± 27, p=0.01) in PA patients than those in EH patients. Postcontrast T1 time (p=0.01) was independently related to global circumferential PDSR after adjusting for age and duration of hypertension in PA patients. Furthermore, plasma aldosterone concentration was negatively associated with myocardial T1 time (R=-0.261, p=0.023) in PA patients. The global circumferential PDSR derived by CMR is decreased, and the diffuse myocardial fibrosis is increased in PA patients compared to those in blood pressure matched EH patients. The severity of cardiac diastolic dysfunction independently relates to the degree of diffuse myocardial fibrosis in PA patients, and the diffuse myocardial fibrosis may be caused by high PAC level. Trial registration number: ChiCTR2000031792.
Context-Based Spatial Decisions in Landscape-Ecological Planning
Authors: Khoroshev Alexander 
Published: February 2021

Abstract
ContextThe vital challenge for the context-based landscape planning is integrating assessments of for both intrinsic properties of a unit and its value in a broad spatial context.ObjectivesThe purpose is to develop the procedure which provides context-based criteria at relevant scales, considers matter flows and dynamic events.MethodsThe methodology involves identification of landscape patterns, revealing mechanisms of radial and lateral relationships, considering changes of landscape patterns, and revealing functioning mechanisms causing directed changes. The proposals proceed from multiplicity of patterns and integrate knowledge of processes in biophysical units, catenas, basins, and matrix elements. The plan was elaborated for the taiga landscape in northern European Russia.ResultsDevelopment of ecological network is aimed at increase of forest cover in basins up to 35% to enhance runoff regulation, prevent soil erosion, and stabilize the riparian biocorridor. The highest significance was assigned to the units that have intrinsic ecological values and contribute to lateral transportation of matter over vast areas. Width of buffer strips intercepting input of pollutants to rivers was adapted to proportions of elements and neighborhoods in catenas. Outside the ecological network, we identified priorities for the multifunctional use of units based on the assessment of drainage conditions, soil quality, and risk of erosion. ConclusionsThe procedure takes into consideration landscape hierarchy and multiplicity of spatial patterns. Integrating multiple models is in compliance with the systemic essence of a landscape ensuring understanding of geodiversity, lateral connections, and the emergent effects that ensure the landscape services for society.
FMRP Attenuates Activity Dependent Modifications in the Mitochondrial Proteome
Authors: Bülow Pernille , Wenner Peter A , Bassell Gary J , Faundez Victor 
Published: February 2021

Abstract
Homeostatic plasticity is necessary for the construction and maintenance of functional neuronal networks, but principal molecular mechanisms required for or modified by homeostatic plasticity are not well understood. We recently reported that homeostatic plasticity induced by activity deprivation is dysregulated in cortical neurons from Fragile X Mental Retardation protein (FMRP) knockout mice [1]. These findings led us to hypothesize that identifying proteins sensitive to activity deprivation and/or FMRP expression could reveal pathways required for or modified by homeostatic plasticity. Here, we report an unbiased quantitative mass spectrometry used to quantify steady-state proteome changes following chronic activity deprivation in wild type and Fmr1-/y cortical neurons. Proteome hits responsive to both activity deprivation and the Fmr1-/y genotype were significantly annotated to mitochondria. We found an increased number of mitochondria annotated proteins whose expression was sensitive to activity deprivation in Fmr1-/y cortical neurons as compared to wild type neurons. These findings support a novel role of FMRP in attenuating mitochondrial proteome modifications induced by activity deprivation.