bluuebunny/crossref_metadata_2025_split huggingface dataset:
Structural insights into the activation of autoinhibited human lipid flippase ATP8B1 upon substrate binding
Authors: Cheng Meng-Ting , Chen Yu , Chen Zhi-Peng , Zhou Cong-Zhao , Hou Wen-Tao , Chen Yuxing 
Published: November 2021

AbstractThe human P4-type ATPase ATP8B1 in complex with the auxiliary noncatalytic protein CDC50A or CDC50B mediates the transport of cell membrane lipids from the outer to the inner membrane leaflet, which is crucial to maintain the asymmetry of membrane lipid. Its dysfunction usually leads to imbalance of bile acid circulation, and eventually causing intrahepatic cholestasis diseases. Here we found that both ATP8B1-CDC50A and ATP8B1-CDC50B possess a higher ATPase activity in the presence of the most favored substrate phosphatidylserine (PS); and moreover, the PS-stimulated activity could be augmented upon the addition of bile acids. The cryo-electron microscopy structures of ATP8B1-CDC50A at 3.36 Å and ATP8B1-CDC50B at 3.39 Å enabled us to capture an unprecedented phosphorylated and autoinhibited state, with the N- and C-terminal tails separately inserting into the cytoplasmic inter-domain clefts of ATP8B1. The PS-bound ATP8B1-CDC50A structure at 3.98 Å indicated the autoinhibited state could be released upon PS binding. Structural analysis combined with mutagenesis revealed the residues that determine the substrate specificity, and a unique positively charged loop in the phosphorylated domain of ATP8B1 for the recruitment of bile acids. Altogether, we updated the Post-Albers transport cycle, with an extra autoinhibited state of ATP8B1, which could be activated upon substrate binding. These findings not only provide structural insights into the ATP8B1-mediated restoration of human membrane lipid asymmetry during bile acid circulation, but also advance our understanding on the molecular mechanism of P-type ATPases.
Morphology of immatures of the thelytokous ant,Monomorium trivialeWheeler (Formicidae: Myrmicinae: Solenopsidini) with descriptions of the extraordinary last-instar queen larvae
Authors: Idogawa Naoto , Gotoh Ayako , Dobata Shigeto 
Published: November 2021

AbstractThe ant genusMonomoriumis one of the most species-rich but taxonomically problematic groups in the hyperdiverse subfamily Myrmicinae. An East Asian species,M. trivialeWheeler, produces both reproductive queens and sterile workers via obligate thelytokous parthenogenesis. Here, we describe the immature forms ofM. trivialebased on light and scanning electron microscopy observations, with a note on the striking caste dimorphism in the last larval instar. The last-instar queen larvae were easily recognized by their large size, “aphaenogastroid” body shape, and rows of doorknob-like tubercles on the lateral and dorsal body surface. This type of queen-specific structure has not been found in ants in general, let alone congeneric species found in Japan. In stark contrast to the queen larvae, worker larvae showed a “pheidoloid” body shape and a body surface similar to other ants. The worker larvae were estimated to have three instars, consistent with previously described congeners. The pupae of both castes had no cocoon, a characteristic commonly described in other Myrmicinae species. In total, the developmental period from egg to adult worker averaged 59 days under 25°C. We discuss possible functions of the tubercles of queen larvae based on previous studies.
Whole-genomes from the extinct Xerces Blue butterfly can help identify declining insect species
Authors: de-Dios Toni , Fontsere Claudia , Renom Pere , Stiller Josefin , Llovera Laia , Uliano-Silva Marcela , Sánchez-Gracia Alejandro , Wright Charlotte , Lizano Esther , Caballero Berta , Navarro Arcadi , Civit Sergi , Robbins Robert K. , Blaxter Mark , Marquès-Bonet Tomàs , Vila Roger , Lalueza-Fox Carles 
Published: November 2021

AbstractThe Xerces Blue (Glaucopsyche xerces) is considered to be the first butterfly to become extinct at global scale in historical times. It was notable for its chalky lavender wings with conspicuous white spots on the ventral wings. The last individuals were collected in their restricted habitat, in the dunes near the Presidio military base in San Francisco, in 1941. We sequenced the genomes of four 80 to 100-year-old Xerces Blue, and seven historical and one modern specimens of its closest relative, the Silvery Blue (G. lygdamus). We compared these to a novel annotated genome of the Green-Underside Blue (G. alexis). Phylogenetic relationships inferred from complete mitochondrial genomes indicate that Xerces Blue was a distinct species that diverged from the Silvery Blue lineage at least 850,000 years ago. Using nuclear genomes, both species experienced population growth during the Eemian interglacial period, but the Xerces Blue decreased to a very low effective population size subsequently, a trend opposite to that observed in the Silvery Blue. Runs of homozygosity and deleterious load in the Xerces Blue were significantly greater than in the Silvery Blue, suggesting a higher incidence of inbreeding. These signals of population decline observed in Xerces Blue could be used to identify and monitor other insects threatened by human activities, whose extinction patterns are still not well known.
Phase of neural oscillations as a reference frame for attention-based routing in visual cortex
Authors: Aboutorabi Ehsan , Ray Sonia Baloni , Kaping Daniel , Shahbazi Farhad , Treue Stefan , Esghaei Moein 
Published: November 2021

AbstractSelective attention allows the brain to efficiently process the image projected onto the retina, selectively focusing neural processing resources on behaviorally relevant visual information. While previous studies have documented the crucial role of the action potential rate of single neurons in relaying such information, little is known about how the activity of single neurons relative to their neighboring network contributes to the efficient representation of attended stimuli and transmission of this information to downstream areas. Here, we show in the dorsal visual pathway of monkeys (medial superior temporal (MST) area) that neurons fire spikes preferentially at a specific phase of the ongoing population beta (~20 Hz) oscillations of the surrounding local network. This preferred spiking phase shifts towards a later phase when monkeys selectively attend towards (rather than away from) the receptive field of the neuron. This shift of the locking phase is positively correlated with the speed at which animals report a visual change. Furthermore, our computational modelling suggests that neural networks can manipulate the preferred phase of coupling by imposing differential synaptic delays on postsynaptic potentials. This distinction between the locking phase of neurons activated by the spatially attended stimulus vs. that of neurons activated by the unattended stimulus, may enable the neural system to discriminate relevant from irrelevant sensory inputs and consequently filter out distracting stimuli information by aligning the spikes which convey relevant/irrelevant information to distinct phases linked to periods of better/worse perceptual sensitivity for higher cortices. This strategy may be used to reserve the narrow windows of highest perceptual efficacy to the processing of the most behaviorally relevant information, ensuring highly efficient responses to attended sensory events.
A mixed blessing of viruses in wastewater treatment plants
Authors: Shi Ling-Dong , Dong Xiyang , Liu Zongbao , Yang Yuchun , Lin Jih-Gaw , Li Meng , Gu Ji-Dong , Zhu Li-Zhong , Zhao He-Ping 
Published: November 2021

AbstractActivated sludge of wastewater treatment plants harbors a very high diversity of both microorganisms and viruses, wherein the latter control microbial dynamics and metabolisms by infection and lysis of cells. However, it remains poorly understood how viruses impact the biochemical processes of activated sludge, for example in terms of treatment efficiency and pollutant removal. Using metagenomic and metatranscriptomic deep sequencing, the present study recovered thousands of viral sequences from activated sludge samples of three conventional wastewater treatment plants. Gene-sharing network indicated that most of viruses could not be assigned to known viral genera, implying activated sludge as an underexplored reservoir for new viruses and viral diversity. in silico Predictions of virus-host linkages demonstrated that infected microbial hosts, mostly belonging to bacteria, were transcriptionally active and able to hydrolyze polymers including starches, celluloses, and proteins. Some viruses encode auxiliary metabolic genes (AMGs) involved in carbon, nitrogen, and sulfur cycling, and antibiotic resistance genes (ARGs) for resistance to multiple drugs. The former group of virus-encoded genes (i.e. AMGs) may enhance the biodegradation of contaminants like starches and celluloses, suggesting a positive role for viruses in strengthening the performance of activated sludge. However, the latter group (i.e. ARGs) would be disseminated to different microorganisms using viruses as gene shuttles, demonstrating the possibility for viruses to facilitate the spread of antibiotic resistance in the environment. Collectively, this study highlights the mixed blessing of viruses in wastewater treatment plants, and deciphers how they manipulate the biochemical processes in the activated sludge, with implications for both environmental protection and ecosystem security.
Enhanced fluorescence from semiconductor quantum dot-labelled cells excited at 280 nm
Authors: McFarlane Mollie , Hall Nicholas , McConnell Gail 
Published: November 2021

AbstractSemiconductor quantum dots (QDs) have significant advantages over more traditional fluorophores used in fluorescence microscopy including reduced photobleaching, long-term photostability and high quantum yields, but due to limitations in light sources and optics, are often excited far from their optimum excitation wavelengths in the deep-UV. Here, we present a quantitative comparison of the excitation of semiconductor QDs at a wavelength of 280 nm, compared to the longer wavelength of 365 nm, within a cellular environment. We report increased fluorescence intensity and enhanced image quality when using 280 nm excitation compared to 365 nm excitation for cell imaging across multiple datasets, with a highest average fluorescence intensity increase of 3.59-fold. We also find no significant increase in photobleaching of QDs associated with 280 nm excitation.
Climatic curves as predictors in MaxEnt niche modeling
Authors: Mikula Ondřej 
Published: November 2021

AbstractEnvironmental niche modelling (ENM) uses different types of variables to predict species occurrence. In widespread use are variables derived from climatic curves, i.e., average annual changes in some climatic parameter. This study shows how to use the climatic curves themselves as ENM predictors. The key step is projection of the curves’ constituent variables on a suitable spline basis, which preserves time-ordering of the variables and supports smoothness of predictions. Complexity of the model is controlled by sensible choice of the spline basis, followed by lasso regularization in model fitting.
Cocaine-induced locomotor activation differs across six sets of inbred mouse substrains
Authors: Gaines Christiann H. , Schoenrock Sarah A. , Farrington Joseph , Lee David F. , Aponte-Collazo Lucas J. , Shaw Ginger , Miller Darla R. , Ferris Martin T. , de Villena Fernando Pardo-Manuel , Tarantino Lisa M. 
Published: November 2021

AbstractCocaine use disorders (CUD) are devastating for affected individuals and impose a significant burden on society, but there are currently no FDA-approved therapies. The development of novel and effective treatments has been hindered by substantial gaps in our knowledge about the etiology of these disorders. The risk for developing a CUD is influenced by genetics, the environment and complex interactions between the two. Identifying specific genes and environmental risk factors that increase CUD risk would provide an avenue for the development of novel treatments.Rodent models of addiction-relevant behaviors have been a valuable tool for studying the genetics of response to drugs of abuse. Traditional genetic mapping using genetically and phenotypically divergent inbred mice has been successful in identifying numerous chromosomal regions that influence addiction-relevant behaviors, but these strategies rarely result in identification of the causal gene or genetic variant. To overcome this challenge, reduced complexity crosses (RCC) between closely related inbred mouse substrains have been proposed as a method for rapidly identifying and validating functional variants. The RCC approach is dependent on identifying phenotypic differences between substrains. To date, however, the study of addiction-relevant behaviors has been limited to very few sets of substrains, mostly comprising the C57BL/6 lineage.The present study expands upon the current literature to assess cocaine-induced locomotor activation in 20 inbred mouse substrains representing six inbred strain lineages (A/J, BALB/c, FVB/N, C3H/He, DBA/2 and NOD) that were either bred in-house or supplied directly by a commercial vendor. To our knowledge, we are the first to identify significant differences in cocaine-induced locomotor response in several of these inbred substrains. The identification of substrain differences allows for the initiation of RCC populations to more rapidly identify specific genetic variants associated with acute cocaine response. The observation of behavioral profiles that differ between mice generated in-house and those that are vendor-supplied also presents an opportunity to investigate the influence of environmental factors on cocaine-induced locomotor activity.
Organizational-level visual identity: an integrative literature review
Authors: Gregersen Magnus Kristian , Johansen Trine Susanne 
Published: November 2021

PurposeThe aim is to review and discuss main conceptualizations, themes and assumptions within organizational-level visual identity (VI) in order to identify potential avenues of theoretical advancement of VI as an independent construct.Design/methodology/approachAn integrative review approach offers a structured, nuanced perspective on the concept by synthesizing extant literature through an iterative, critical and qualitative process.FindingsThe synthesis identifies three overlapping terms [corporate visual identity (CVI), visual brand identity (VBI) and VI] and two main themes (visual consistency and authenticity). The dominant assumptions underpinning consistency and authenticity are challenged by alternative understandings, which provide a platform for perceiving visual consistency and authenticity in new ways.Research limitations/implicationsThe review offers an overview of organizational-level VI that helps define the concept as well as critical reflections which open up for additional research avenues that may develop it and point to potential areas for exploration.Practical implicationsThe review provides practitioners with a platform for discussing how to approach visual identities with regards to consistency and authenticity.Originality/valueThe review contributes with a synthesis of VI literature covering 50 years. It offers a structured presentation of and critical discussion on the underlying, dominant assumptions. By challenging these dominant assumptions, a palette of future research opportunities, with potentials to nuance and develop the concept as a unique construct, are presented.
Uncovering value creation in innovation ecosystems: paths towards shared value
Authors: Arena Marika , Azzone Giovanni , Piantoni Giulia 
Published: November 2021

PurposeAlthough innovation ecosystems (IEs) are generally considered capable of creating shared value (SV), this potentiality has often been taken for granted and not deeply analysed, yet. As a result, in the literature, there is not a framework that defines the process of SV creation in IEs or which aspects should be considered for understanding it. Moving from these considerations, this paper aims to propose a conceptual model of how IEs can create SV, identifying the main building blocks of the process and the aspects that characterize these building blocks.Design/methodology/approachThe authors reviewed the literature on IEs and value creation over the last 15 years, by structurally analysing 120 articles. On the basis of such review, the authors identified main dimensions of analysis focusing on the conceptualization of SV in IEs.FindingsFirst, the authors developed a conceptual model relying on a process-based logic and framing the SV creation in terms of inputs, here intended as four key characteristics (actors, structure, governance and relations), internal processes (strategies and internal mechanisms) and outputs (the value created). Second, each element of value creation is explored, highlighting the main evidence emerging from prior studies in connection to each block.Originality/valueThis paper drives the identification of some relevant relationships that connect the characteristics of the IEs, the strategies and the internal mechanisms to the output of the process, i.e. the SV created.
Morality in the era of smart devices
Authors: Sui Jiarui , Mo Tiantian 
Published: November 2021

PurposeDoes using smart devices change people's moral standards? The objective of this paper is to investigate how people's moral behavioral intentions while employing smart devices are modulated by their socioeconomic status (SES; as measured by educational level and income).Design/methodology/approachParticipants were randomly assigned to either the smart devices condition or the non-smart devices condition, and their moral standards was measured by the adapted Moral Foundations Questionnaire. Data were collected from both China and the UK.FindingsIndividuals' SES moderated people's moral standards when using smart devices. Specifically, when employing smart devices (vs non-smart devices), moral standards declined for low-SES individuals. However, the effect of employing smart devices was not significant for high-SES individuals. This suggests that certain demographics may be more inclined to harm others with smart devices.Practical implicationsIn emerging markets, the widespread of smart devices in workplace may lower consumers' and employees' moral standards for certain demographics. Managers and marketers need to be aware of this erosion of morality and employ some preventive measures in advance.Originality/valueThis paper examined morality in the era of smart devices. Even though the use of smart devices has become a norm in developed markets, smart devices usage is still on the rise in emerging markets. The authors findings enhance the understanding of moral behaviors and contribute to the knowledge of how smart devices are changing human behaviors.
Shaking table test on single segment prefabricated concrete bridge pier connected by grouting sleeve
Authors: Xu Junming , Jia Yanmin , Liang Dongwei 
Published: November 2021

PurposePrefabricated pier technology has the advantages of quick construction time, relatively little traffic interference and relatively small environmental impact. However, its applicability under earthquake conditions is not yet fully understood. The seismic performance and influence parameters of a prefabricated concrete pier connected by embedded grouting sleeve (GS) in a pile cap are investigated in this study.Design/methodology/approachTwo prefabricated pier scale model specimens with different reinforcement anchorage lengths and two comparative cast-in-place (CIP) pier model specimens are designed and manufactured for a seismic simulation shaking table. With the continuous increase of input ground motion strength, the changes in basic dynamic characteristics, damage development, acceleration and displacement variation laws, and pier bottom strain responses are compared among the specimen. The finite element software ABAQUS is used to simulate the test pier.FindingsThe crack location of the two prefabricated pier specimens is almost the same as that of the CIP pier specimens; CIP pier specimens show more penetrated cracks than prefabricated pier specimens, as well as an earlier crack penetration time. The acceleration, displacement and strain response of the CIP pier specimens are more affected by earthquake activity than those of the prefabricated pier specimens. The acceleration, displacement and strain responses of the two prefabricated piers are nearly identical. The finite element results are in close agreement with the acceleration and displacement response data collected from the test, which verifies the feasibility of the finite element model established in ABAQUS.Originality/valueA GS connection method is adopted for the prefabricated pier, and on the premise of meeting the minimum reinforcement anchorage length required by the code, this study explores the influences of different reinforcement anchorage lengths on the seismic performance of prefabricated piers in high-intensity areas. A shaking table loading test is used to simulate the real changes of the structure under the earthquake. This work may provide a valuable reference for the design and seismic performance analysis of prefabricated pier, particularly in terms of seismic stability.
Storytelling in online shops: the impacts on explicit and implicit user experience, brand perceptions and behavioral intention
Authors: Karampournioti Evmorfia , Wiedmann Klaus-Peter 
Published: November 2021

PurposeThis paper examines in detail how the use of storytelling with parallax technology can influence the user experience (UX) in online shops as well as brand- and behavior-relevant variables. Furthermore, this study analyzes the causal relationships between UX, brand attitudes and brand-related behavioral intentions in terms of purchase intention and price premiums. Explicit and implicit paths of human information processing are considered.Design/methodology/approachA sample of 266 respondents completed a web-based experiment under two conditions (text-based vs parallax storytelling online shop). An existing and operational online shop was used. The causal relationships were assessed by using partial least squares structural equation modeling (PLS-SEM). To measure implicit information processing, a single category implicit association test was applied.FindingsBy applying the storytelling technique with parallax scrolling, the online shop increased visitors' UX on explicit and implicit information processing levels and increased the online shop's overall perceived attractiveness. Storytelling with parallax motion enables an efficient transmission of brand-related associations to consumers' minds, enhances their explicit and implicit brand attitudes and increases their willingness to pay a higher price. Moreover, this study provides empirical evidence on the effects of UX on brand-related measures by applying PLS-SEM and thus reveals a causal chain of effects from UX on online shop attractiveness, brand attitude and behavioral intentions. Again, explicit and implicit perceptions were considered.Originality/valueScience and practice are increasingly emphasizing that storytelling emotionalizes content, which facilitates effective communication and builds strong relationships with customers. Little evidence exists about its efficient implementation in an online shopping context and in fulfilling hedonic and pragmatic needs throughout the online journey. This study provides novel insights into managing online shoppers' UX, brand-related perceptions and behavioral intentions with the optimal use of techniques to implement storytelling. Furthermore, this is one of the first studies to holistically consider the human perception of online shops by drawing on theories and methods of psychology, marketing, consumer behavior, brand research and consumer neuroscience and considering explicit and implicit information processing in terms of hedonic and pragmatic UX and brand-related measures.