bluuebunny/crossref_metadata_2025_split huggingface dataset:
Correlation between the bacterial community and flavour of fermented fish
Authors: Wang Jin-Le , Zhao Feng , Cairang Zuo-Ma , Li Xiao-Yi , Kong Jie , Zeng Shi-Yu , Zhang Mei-Yan , Zhao Zhen-Xin , Zhang Xiao-Ping 
Published: October 2021

Common carp is a fish species of economic importance in China;traditionally it is mostly salt-fermented. In the fermenting process, the bacterial community of spontaneously fermented fish is important for its flavour and quality. However, very few studies have been conducted about the relationship between bacterial community and development of flavour involved in the salt-fermentation of carp. Therefore, we explored this relationship by determining the flavour components, including amino acids, and changes in volatile flavour and bacterial metabolite. Samples were taken during fermentation on the days 0, 10, 20, 30, 40 and 50. The second-generation 16S recombinant DNA (rDNA) sequencing was performed to analyze the composition of bacteria. Contents of amino acids were determined by reverse-phase high-performance liquid chromatography combined with ultraviolet detection. The volatile components were analyzed with solid-phase microextraction–gas chromatography-mass spectrometry. Enterococcus, Lactobacillus, Lactococcus, Leuconostoc, and Staphylococcus were the dominant bacteria. The bidirectional orthogonal partial least squares approach was used to analyze the correlation between bacterial succession and flavour component dynamics. This study would help to better understand the role of bacteria in the fermented fish meat flavour and support the industrial production of fermented fish.
Clinical profile of new borns with trachea-esophageal fistula and esophageal atresia and factors associated with outcome at a tertiary care centre
Authors: Keerthidarshini  , R Sandeep , Shanbag Gopalkrishna 
Published: October 2021

Although trachea-esophageal fistula (TEF) and esophageal atresia (EA) is rare, it is one of the most common congenital anomaly. There is paucity of data due to rarity of condition. More and more data on clinical profile, complications, and outcome are needed to guide the future research. To study clinical profile of newborns with TEF and EA and factors associated with outcome.Hospital based prospective study was carried out among 21 newborns with TEF and EA. Diagnosis was confirmed by investigations like X-ray chest after passing nasogastric catheter. Depending upon final diagnosis type of surgery required was determined and procedure was carried out as per the standard guidelines and operating techniques. All cases were followed till the complete post-operative period and the complications and outcome was assessed. Outcome was classified as death or discharge.Males outnumbered females giving a male to female ratio of 2.5:1. 57.1% did not require ventilator pre-operatively. 61.9% of the cases had no associated abnormality. The most common type of surgery required and performed was tracheoesophageal repair in 52.4% of the cases. Two cases (9.5%) died during the post-operative period. 14 cases (66.7%) recovered and were discharged. Most common complication was anastomotic leak (57.1%). Term status and associated abnormalities were not found to be associated with outcome like death (p&#62;0.05). Trachea-esophageal fistula and esophageal atresia was more common in males. Tracheoesophageal repair was commonly required surgery. Recovery rate was good. Anastomotic leak was most common complication. Outcome like death was not associated with term status and associated abnormalities.
Infertility: A case study
Authors: Kamei Almond , Laskar Nasima 
Published: October 2021

Infertility is a disease of the male or female reproductive system defined by the failure to achieve a pregnancy after 12 months or more of regular unprotected sexual intercourse. It affects millions of people of reproductive age worldwide. Fertility care encompasses the prevention, diagnosis and treatment of infertility. It is vital part of the nurse to offer psychological support to the couple and provide nursing Management based on the priority.
Los límites difusos entre compuestos sintagmáticos, colocationes y locuciones
Authors: Galvez Vidal Alba María , Sevilla Munoz Manuel 
Published: October 2021

Desde un punto de vista formal, semántico y funcional, existen similitudes entre compuestos sintagmáticos (CS) y otros sintagmas fijos, como colocaciones y locuciones, por lo que no siempre es fácil diferenciarlos. Es posible encontrar un mismo sintagma clasificado como locución, colocación y CS en función de la obra lexicográfica o fraseográfica consultada (mano dura, por ejemplo). García-Page (En torno a, «Hombre rana»), entre otros autores, ha mostrado su preocupación por estas similitudes; Osorio y Serra realizaron una propuesta para distinguir entre locuciones y CS; y Corpas (51) establece la diferencia entre colocaciones y locuciones según su proceso de fijación.
En este estudio, pretendemos delimitar el concepto de CS en comparación con colocaciones y locuciones desde un enfoque fraseológico, partiendo de la teoría de Pamies, quien considera los CS como unidades fraseológicas (UF). Para ello, planteamos dos criterios relacionados con la fijación de los CS: que se hayan acuñado de manera artificial debido a la necesidad de nombrar una realidad ontológica por primera vez y que esa necesidad surja en un ámbito especializado. Comprobamos la validez de estos dos criterios en un corpus de sintagmas fijos extraído de tres novelas españolas.
Predictors and Outcomes of Postoperative Hemoglobin of <8 g/dL in Total Joint Arthroplasty
Authors: Chaudhry Yash P. , MacMahon Aoife , Rao Sandesh S. , Mekkawy Kevin L. , Toci Gregory R. , Oni Julius K. , Sterling Robert S. , Khanuja Harpal S. 
Published: October 2021

Background:
Restrictive transfusion practices have decreased transfusions in total joint arthroplasty (TJA). A hemoglobin threshold of <8 g/dL is commonly used. Predictors of this degree of postoperative anemia in TJA and its association with postoperative outcomes, independent of transfusions, remain unclear. We identified predictors of postoperative hemoglobin of <8 g/dL and outcomes with and without transfusion in TJA.

Methods:
Primary elective TJA cases performed with a multimodal blood management protocol from 2017 to 2018 were reviewed, identifying 1,583 cases. Preoperative and postoperative variables were compared between patients with postoperative hemoglobin of <8 and ≥8 g/dL. Logistic regression and receiver operating characteristic curves were used to assess predictors of postoperative hemoglobin of <8 g/dL.

Results:
Positive predictors of postoperative hemoglobin of <8 g/dL were preoperative hemoglobin level (odds ratio [OR] per 1.0-g/dL decrease, 3.0 [95% confidence interval (CI), 2.4 to 3.7]), total hip arthroplasty (OR compared with total knee arthroplasty, 2.1 [95% CI, 1.3 to 3.4]), and operative time (OR per 30-minute increase, 2.0 [95% CI, 1.6 to 2.6]). Negative predictors of postoperative hemoglobin of <8 g/dL were tranexamic acid use (OR, 0.42 [95% CI, 0.20 to 0.85]) and body mass index (OR per 1 kg/m2 above normal, 0.90 [95% CI, 0.86 to 0.94]). Preoperative hemoglobin levels of <12.4 g/dL in women and <13.4 g/dL in men best predicted postoperative hemoglobin of <8 g/dL. Overall, 5.2% of patients with postoperative hemoglobin of 7 to 8 g/dL and 95% of patients with postoperative hemoglobin of <7 g/dL received transfusions. Patients with postoperative hemoglobin of <8 g/dL had longer hospital stays (p < 0.001) and greater rates of emergency department visits or readmissions (p = 0.001) and acute kidney injury (p < 0.001). Among patients with postoperative hemoglobin of <8 g/dL, patients who received transfusions had a lower postoperative hemoglobin nadir (p < 0.001) and a longer hospital stay (p = 0.035) than patients who did not receive transfusions.

Conclusions:
Postoperative hemoglobin of <8 g/dL after TJA was associated with worse outcomes, even for patients who do not receive transfusions. Optimizing preoperative hemoglobin levels may mitigate postoperative anemia and adverse outcomes.

Level of Evidence:
Prognostic Level IV. See Instructions for Authors for a complete description of levels of evidence.
Lymphedema Is a Significant Risk Factor for Failure After Primary Total Hip Arthroplasty
Authors: Rainer William G. , Kolz Joshua M. , Wyles Cody C. , Houdek Matthew T. , Perry Kevin I. , Lewallen David G. 
Published: October 2021

Background:
Lymphedema is a chronic disease characterized by fluid buildup and swelling that can lead to skin and soft-tissue fibrosis and recurring soft-tissue infections. Literature with regard to the increased risk of complications following a surgical procedure in patients with lymphedema is emerging, but the impact of lymphedema in the setting of primary total hip arthroplasty (THA) remains unknown. The purpose of this study was to review outcomes following primary THA performed in patients with lymphedema compared with a matched cohort without lymphedema.

Methods:
Using our institutional total joint registry and medical records, we identified 83 patients (57 were female and 26 were male) who underwent THA with ipsilateral lymphedema. For comparison, these patients were matched 1:6 (based on sex, age, date of the surgical procedure, and body mass index [BMI]) to a group of 498 patients without lymphedema who underwent primary THA for osteoarthritis. Subsequently, postoperative complications and implant survivorship were evaluated for each group. The mean follow-up for each group was 6 years. Survivorship was compared between cohorts using Kaplan-Meier methodology and included both survivorship free of infection and survivorship free of reoperation or revision. Univariate Cox regression analysis was utilized to assess the association between patient factors for the time to event outcomes noted above.

Results:
In patients with a history of lymphedema, there was an increased risk of complications (hazard ratio [HR], 1.97; p < 0.01), including reoperation for any cause (HR, 3.16; p < 0.01) and postoperative infection (HR, 4.48; p < 0.01). The 5-year infection-free survival rate was 90.3% for patients with lymphedema compared with 97.7% for patients without lymphedema (p < 0.01).

Conclusions:
Patients with lymphedema are at increased risk for complications, including reoperation and infection, following primary THA. These data emphasize the importance of appropriate preoperative counseling in this population and should encourage efforts to identify methods to improve outcomes, including further investigation of the effects of preoperative optimization of lymphedema prior to THA and methods for improved perioperative management.

Level of Evidence:
Prognostic Level III. See Instructions for Authors for a complete description of levels of evidence.
Volar Locking Plate Versus Dorsal Locking Nail-Plate Fixation for Dorsally Displaced Unstable Extra-Articular Distal Radial Fractures
Authors: Eikrem Morten , Brannsten Hege , Bjørkøy Dagfinn , Lian Tom , Madsen Jan Erik , Figved Wender 
Published: October 2021

Background:
The use of low-profile dorsal and volar locking plates for distal radial fracture surgery has improved results and lowered the complication rate compared with older plate designs. The purpose of the present randomized controlled trial was to compare patient-reported outcomes as well as radiographic and functional results between patients who underwent stabilization with a volar locking plate or a dorsal locking nail-plate for the treatment of dorsally displaced unstable extra-articular distal radial fractures.

Methods:
One hundred and twenty patients ≥55 years of age were randomized to surgery with either a volar locking plate or a dorsal locking nail-plate and were assessed at 2 weeks, 6 weeks, 3 months, 6 months, and 1 year. The primary outcome was the abbreviated version of the Disabilities of the Arm, Shoulder and Hand (QuickDASH) score. Secondary outcomes were the Patient-Rated Wrist Evaluation (PRWE), EuroQol 5 Dimensions (EQ-5D) index and visual analog scale (VAS), range of motion, grip strength, radiographic measurements, and complication rate.

Results:
The median age was 66 years (range, 55 to 88 years). The rate of follow-up was 97%. There was no clinically important difference between the groups at any point during follow-up. Patients in the volar locking plate group had better mean QuickDASH scores at 6 weeks, 6 months, and 1 year. However, the differences were small (5.8 vs. 11.3 points at 1 year; mean difference, −5.5 points [95% confidence interval (CI), −9.9 to 1.2]; p = 0.014), which is lower than any proposed minimum clinically important difference (MCID). The difference in PRWE scores was also lower than the MCID (1.0 vs. 3.5 at 1 year; mean difference, −2.5 [95% CI, −4.4 to 0.6]; p = 0.012). The dorsal locking nail-plate group had slightly better restoration of volar tilt (p = 0.011). EQ-5D index, EQ-5D VAS, range of motion, grip strength, and complication rates were similar.

Conclusions:
We found no clinically relevant difference between the volar locking plate and dorsal locking nail-plate groups after 1 year or in the time period up to 1 year. A dorsal locking nail-plate can therefore be an alternative method for the treatment of these unstable fractures or in cases in which a dorsal approach is preferable over a volar approach.

Level of Evidence:
Therapeutic Level I. See Instructions for Authors for a complete description of levels of evidence.
Same-species Contamination Detection With Variant Calling Information From Next-generation Sequencing
Authors: Jiang Tao , Buchkovich Martin , Motsinger-Reif Alison 
Published: October 2021

Abstract
Background: Same-species contamination detection is an important quality control step in genetic data analysis. Due to a scarcity of methods to detect and correct for this quality control issue, same-species contamination is more difficult to detect than cross-species contamination. We introduce a novel machine learning algorithm to detect same-species contamination in next-generation sequencing (NGS) data using a support vector machine (SVM) model. Our approach uniquely detects contamination using variant calling information stored in variant call format (VCF) files for DNA or RNA. Importantly, it can differentiate between same-species contamination and mixtures of tumor and normal cells.In the first stage, a change-point detection method is used to identify copy number variations (CNVs) and copy number aberrations (CNAs) for filtering. Next, single nucleotide polymorphism (SNP) data is used to test for same-species contamination using an SVM model. Based on the assumption that alternative allele frequencies in NGS follow the beta-binomial distribution, the deviation parameter ρ is estimated by the maximum likelihood method. All features of a radial basis function (RBF) kernel SVM are generated using publicly available or private training data. Results: We demonstrate our approach in simulation experiments. The datasets combine, in silico, exome sequencing data of DNA from two lymphoblastoid cell lines (NA12878 and NA10855). We generate VCF files using variants identified in these data and then evaluate the power and false-positive rate of our approach. Our approach can detect contamination levels as low as 5% with a reasonable false-positive rate. Results in real data have sensitivity above 99.99% and specificity of 90.24%, even in the presence of degraded samples with similar features as contaminated samples. We provide an R software implementation of our approach.Conclusions: Our approach addresses the gap in methods to test for same-species contamination in NGS. Due to its high sensitivity for degraded samples and tumor-normal samples, it represents an important tool that can be applied within the quality control process. Additionally, the user-friendly software has the unique ability to conduct quality control using the VCF format.
COL5A3 is a Prognostic Biomarker and Correlated with Immune Infiltrates in Pancreatic Cancer
Authors: Li Yongjie , Zeng Min , Wang Ting , Jiang Feng 
Published: October 2021

Abstract
Pancreatic cancer is a malignant tumor of digestive system with high fatality rate, and its prognosis is very poor. Type Ⅴ collagen α3 (COL5A3) is highly expressed in a variety of tumor tissues, but its prognostic value and immune infiltration in pancreatic cancer are still unclear. Therefore, we evaluated the prognostic role of COL5A3 in pancreatic cancer and its correlation with immune infiltration. The transcriptional expression profiles of COL5A3 in pancreatic cancer and normal tissues were downloaded from the Cancer Genome Atlas (TCGA). In the GEO (Gene expression omnibus) dataset (GSE16515), we compared the expression of COL5A3 in normal and tumor tissues. The expression of COL5A3 protein was evaluated by the human protein atlas (THPA). The effect of COL5A3 on survival was evaluated by Kaplan-Meier method. Receiver operating characteristic (ROC) curve was used to distinguish pancreatic cancer from paracancerous normal tissues. Protein-protein interaction (PPI) network was constructed by the STRING. Use the "ClusterProfiler" package for functional enrichment analysis. Tumor immune estimation resource (TIMER) and tumor-immune system interaction database (TISIDB) were used to determine the relationship between COL5A3 mRNA expression and immune infiltration. Compared with normal tissues, COL5A3 is highly expressed in pancreatic cancer tissues. The high expression of COL5A3 is related to the poor clinicopathological features and poor prognosis of pancreatic cancer. Kaplan-Meier survival analysis showed that the prognosis of pancreatic cancer patients with high expression of COL5A3 was worse than that of patients with low expression of COL5A3. ROC curve shows that COL5A3 has high sensitivity and specificity in the diagnosis of pancreatic cancer. Correlation analysis showed that the expression of COL5A3 mRNA was related to immune cell infiltration. This study reveals for the first time that COL5A3 may be a new prognostic biomarker related to immune infiltration and provide a new target for the diagnosis and treatment of pancreatic cancer.
Long-term Evaluation Parameters in GNE Myopathy: A Five-year Observational Follow-up Natural History Study
Authors: Mori-Yoshimura Madoka , Yajima Hiroyuki , Oya Yasushi , Mizuno Katsuhiro , Noguchi Satoru , Nishino Ichizo , Takahashi Yuji 
Published: October 2021

Abstract

BackgroundA number of clinical trials targeting GNE myopathy patients have been conducted. However, useful clinical parameters for post-marketing surveillance and long-term clinical observation have not yet been established. ObjectiveWe conducted a 5-year observational follow-up natural history study to identify evaluation parameters which may be useful for the long-term observation of GNE myopathy patients. MethodsThirty-three genetically-confirmed GNE myopathy patients were recruited and evaluated at study entry (baseline) and yearly in a 5-year follow-up. Hand-held dynamometer measurements of knee extension strength, grip power, and pinch power, summed Manual Muscle Testing (MMT) score of 17 muscles, Gross Motor Function Measure (GMFM), 6-minute walk test, percent vital capacity and percent force vital capacity (%FVC), lean body mass (whole body, arms, and legs), creatine kinase (CK), Barthel Index, modified Rankin Scale, and SF-36 national standard scores were examined. ResultsOf the 33 patients, 22 (66%) completed evaluations for the entire 5-year follow-up period. These patients had a significant reduction in summed MMT score (p=0.001), GMFM (p=0.001), grip power (p=0.013), pinch power (p<0.001), CK (p=0.030), %FVC (p<0.001), leg lean body mass (p=0.040), and the Physical Functioning subscale score of the SF-36 (p=0.015) at the 5th year evaluation relative to baseline. Among these parameters, summed MMT score, GMFM, pinch power, and %FVC showed significant changes even in non-ambulant patients.ConclusionsMMT, GMFM, pinch power, CK, %FVC, lean body mass, and Physical Functioning subscale score of the SF-36 are useful parameters for the long-term evaluation of GNE myopathy patients.
Hydrographic Processes in a Tropical Coastal Lagoon on Western Bay of Bengal
Authors: Sahoo Rabindra Kumar , Sil Sourav , Mandal Samiran , Pradhan Subhasis , Baliarsingh Sanjiba Kumar , Samal Rabindronath 
Published: October 2021

Abstract
In this article, hydrographic processes of a tropical coastal lagoon is studied that control inherent biological mechanisms of the lagoon environment. Realizing the interest of environmentalists over physio-chemical studies of a wetland tropical wetland system on the western boundary of the Bay of Bengal, a high-resolution intensive vertical hydrographic field campaign was carried during monsoon to uncover peculiarity in vertical hydrographic processes that was long-awaited to address many environmental issues. Vertical hydrographic profiles on spatio-temporal scale were made at nine stations in a zonal direction of the Chilika lagoon system. Results of vertical variability of salinity showed the presence of higher saline water over less saline water in the central-western region. The higher and lower water temperature in the western and eastern parts of the lagoon, respectively, indicated temperature dipole between the two regions. The encapsulation of water mass having higher temperature by the water of lower temperature at the central region resulted evolution of thermal inversion. The highest dissolved oxygen concentration was observed in the sub-surface layers of the western part of the lagoon. However, a layer of near-hypoxia occurred below 1.5 m depth in the central region. This study proposes comprehensive inter-seasonal studies to address the vertical variability of biogeochemical parameters and the fate of organic flux.
The concept of the “Hexahedron” in the supratotal resection of the frontal gliomas: A technical note
Authors: Cai Pu , Bai Gang , Peng Jun , Li Yun , Che Shanli , Lan Fei , Yang Haifeng 
Published: October 2021

Abstract
OBJECTIVE

To evaluate the value of the concept of the “Hexahedron” in the supratotal resection (SPTR) of frontal gliomas in both dominant and nondominant hemispheres .
METHODS

All consecutive patients who underwent SPTR for frontal gliomas under the guidance from the concept of the “Hexahedron” were retrospectively analysed for lesion location, pathology, extent of resection (EOR), and complications from May 2020 to June 2021. Volumetric EOR was measured and classified as SPTR, (in which the volume of the postoperative cavity was larger than the preoperative tumour volume), gross total resection (GTR, > 95% by volume) or subtotal resection (STR, ≤ 95% by volume) after independent radiological review.
RESULTS

Six men and two women (mean age: 47.13 years; range: 26–69 years) were included. All eight patients underwent frontal craniotomy combined frontotemporal craniotomy for resection of frontal gliomas. Neuropathological examination confirmed a diagnosis of glioblastoma WHO Grade IV in 4 patients, anaplastic oligodendroglioma WHO Grade III in 1, anaplastic astrocytoma WHO Grade III in 2 and diffuse astrocytoma WHO Grade II in 1. SPTR was achieved in six patients and STR was achieved in two. The main postoperative complications were contralateral paresis in 2 patients and memory disturbances in 1 patient. There were no cases of rebleeding or secondary operation during hospitalization.
CONCLUSIONS

In the presented eight cases the concept of the “Hexahedron” allowed for safe surgical supratotal resection of frontal gliomas.