bluuebunny/crossref_metadata_2025_split huggingface dataset:
Horseradish Peroxidase-Conjugated-Nanobody-Based cELISA For The Rapid and Sensitive Clinical Detection of African Swine Fever Virus Antibodies
Authors: Zhao Huijun , Ren Jiahui , Wu Shuya , Du Yongkun , Wan Bo , Zhuang Guoqing , Ji Pengchao , Wu Yanan , Zhang Angke , Zhang Gaiping 
Published: November 2021

Abstract
Background: African swine fever (ASF), which is caused by the ASF virus (ASFV), is a highly contagious hemorrhagic disease that affects pigs and has the potential to cause mortality in almost 100% of domestic pigs and wild boars. Due to the lack of an effective vaccine, the control of ASF must depend on early, efficient, cost-effective detection and strict control and elimination strategies. Traditional molecular and serological testing methods are generally associated with high testing costs, complex operations and high technical requirements. As a promising alternative diagnostic tool to traditional antibodies, nanobodies (Nb) have the advantages of simpler and faster generation, good stability and solubility, and high affinity and specificity. The application of Nbs in the detection of ASFV antibodies in the serum has not yet been reported, to the best of our knowledge. Results: Using a phage display technology, one specific Nb against the ASFV p54 protein that exhibited high specificity and affinity to the protein, Nb8, was successfully screened. A HEK293T cell line stably expressing Nb8-horseradish peroxidase (HRP) fusion protein was established using the lentiviral expression system. Following the optimization of the reaction conditions, the Nb8-HRP fusion protein was successfully used to establish a competitive enzyme-linked immunosorbent assay (cELISA) to detect ASFV-specific antibodies in pig serum, for the first time. The cut-off value for the cELISA was 15.78%. A total of 209 serum samples were tested using the developed cELISA and a commercial ELISA kit. The specificity of the cELISA was 98.97%, and the limit of detection was 1:320 in inactivated ASFV antibody-positive reference serum samples, with the coincidence rate between the two methods being 98.56%. Conclusions: A specific, sensitive and repeatable cELISA was successfully developed based on the unique Nb8 as a probe, providing a promising method for the detection of anti-ASFV antibodies in clinical pig serum.
Contribution of Bioenergy to the Modern Energy Services in Ethiopia
Authors: Megersa Shasho , Jemal Kedir , Desisa Buzayehu 
Published: November 2021

Abstract
Biomass based traditional energy has been the main energy supply in Ethiopia. Efforts are being made to shift to modern bioenergy utilization but the level of contribution of modern bioenergy to the total energy supply of the country’s supply is not computed. In this synthesis we described the contribution of bioenergy to modern energy utilization in the country. Data used here was retrieved from the country’s official reports and published literatures. Access to modern cooking services in the country was particularly focused on and both biogas feedstock productivities and biogas processing efficiencies were calculated. Herfindahl Index (HI) was calculated to observe the change in the diversity of the total primary energy supply due to bioenergy in the country. Results indicated that only a few households, 10%, had access to modern bioenergy services. Less than 0.10% of households have a biogas digester. The HI values showed the low diversity of the energy supply and the very limited contribution of modern bioenergy. This synthesis indicated that the contribution of modern bioenergy to the energy supply of the country is very low. Very low difference was observed between Herfindahl Indexes with and without considering modern bioenergy in the total primary energy supply (TPES) of the country is also found to be insignificant. Results found indicated lower diversity of the energy supply of Ethiopia and very limited contribution of modern bioenergy to the diversity and security of the energy supply.
Halpern-type relaxed inertial algorithms with Bregman divergence for solving variational inequalities
Authors: Jolaoso Lateef Olakunle , Sunthrayuth Pongsakorn , Cholamjiak Prasit , Cho Yeol Je 
Published: November 2021

Abstract
It is well-known that the use of Bregman divergence is an elegant and effective technique for solving many problems in applied sciences. In this paper, we introduce and analyze two new inertial-like algorithms with Bregman divergence for solving pseudomonotone variational inequalities in a real Hilbert space. The first algorithm is inspired by Halpern -type iteration and subgradient extragradient method and the second algorithm is inspired by Halpern -type iteration and Tseng's extragradient method. Under suitable conditions, the strong convergence theorems of the algorithms are established without assuming the Lipschitz continuity and the sequential weak continuity of any mapping. Finally, several numerical experiments with various types of Bregman divergence are also performed to illustrate the theoretical analysis. The results presented in this paper improve and generalize the related works in the literature.
Hypoxia-Related Long Non-Coding RNA Signature Predicts the Prognosis of Breast Cancer
Authors: Huo Jinlong , Shen Shuang , Chen Chen , Qu Rui , Guo Youming , Ou Dong 
Published: November 2021

Abstract
Background: Breast cancer(BC) is the most common tumour in women. Hypoxia stimulates metastasis in cancer and is linked to poor patient prognosis.Methods: We screened prognostic-related lncRNAs(Long Non-Coding RNAs) from the Cancer Genome Atlas (TCGA) data and constructed a prognostic signature based on hypoxia-related lncRNAs in BC.Results: We identified 21 differentially expressed lncRNAs associated with BC prognosis. Kaplan Meier survival analysis indicated a significantly worse prognosis for the high-risk group(P<0.001). Moreover, the ROC-curve (AUC) of the lncRNAs signature was 0.700, a performance superior to other traditional clinicopathological characteristics. Gene set enrichment analysis (GSEA) showed many immune and cancer-related pathways and in the low-risk group patients. Moreover, TCGA revealed that functions including activated protein C (APC)co-inhibition, Cinnamoyl CoA reductase(CCR),check-point pathways, cytolytic activity, human leukocyte antigen (HLA), inflammation-promotion, major histocompatibility complex(MHC) class1, para-inflammation, T cell co-inhibition, T cell co-stimulation, and Type Ⅰ and Ⅱ Interferons (IFN) responses were significantly different in the low-risk and high-risk groups. Immune checkpoint molecules such as ICOS, IDO1, TIGIT, CD200R1, CD28, PDCD1(PD-1), were also expressed differently between the two risk groups. The expression of m6A-related mRNA indicated that YTHDC1, RBM15, METTL3, and FTO were significantly between the high and low-risk groups.Additionally, immunotherapy in patients with BC from the low-risk group yielded a higher frequency of clinical responses to anti-PD-1/PD-L1 therapy or a combination of anti-PD-1/PD-L1and anti-CTLA4 therapies.Except for lapatinib, the results also show that a high-risk score is related to a higher half-maximal inhibitory concentration (IC50) of chemotherapy drugs.Conclusion: A novel hypoxia-related lncRNAs signature may serve as a prognostic model for BC.
The Pro-Inflammatory Effect of Staphylokinase Contributes to Community-Associated Staphylococcus Aureus Pneumonia
Authors: Wang Yanan , Zhao Na , Jian Ying , Liu Yao , Zhao Lin , He Lei , Liu Qian , Li Min 
Published: November 2021

Abstract
Pneumonia caused by community-associated Staphylococcus aureus (CA-SA) has high morbidity and mortality. Pathogens can express a variety of virulence factors to promote infection. The game between the host immune system and pathogens determines the degree of infection and tissue damage, but its mechanism has not been well explored. In this study, we found that staphylokinase (SAK) is highly expressed in CA-SA lineage ST398 and promotes lung infection in both wild-type and cathelicidins-related antimicrobial peptide CRAMP knockout (CRAMP−/−) mice. Furthermore, our results showed that SAK can activate the host's innate immune inflammatory response. Importantly, SAK can promote the activation of NLRP3 inflammasome by increasing the production of ROS. This may be one of the important mechanisms of SAK aggravating CA-SA pneumonia after excluding its function of promoting fibrinolysis and neutralizing CRAMP. Our results emphasize the importance of controlling inflammation in acute lung infections, and also provides new insights into the pathogenesis of highly virulent CA-SA.
Systemic and central nervous system neuroinflammatory signatures of neuropsychiatric symptoms and related cognitive decline in older people
Authors: Clark Christopher , Richiardi Jonas , Maréchal Bénédicte , Bowman Gene L. , Dayon Loïc , Popp Julius 
Published: November 2021

Abstract
Background

Neuroinflammation may contribute to psychiatric symptoms in older people, in particular in the context of Alzheimer’s disease (AD). We sought to identify systemic and central nervous system (CNS) inflammatory alterations associated with neuropsychiatric symptoms (NPS); and to investigate their relationships with AD pathology and clinical disease progression.
Methods

We quantified a panel of 38 neuroinflammation and vascular injury markers in paired serum and cerebrospinal fluid (CSF) in a cohort of cognitively normal and impaired older subjects. We performed neuropsychiatric and cognitive evaluations and measured CSF biomarkers of AD pathology. Multivariate analysis determined serum and CSF neuroinflammatory alterations associated with NPS, considering cognitive status, AD pathology, and cognitive decline at follow-up visits.
Results

NPS were associated with distinct inflammatory profiles in serum, involving eotaxin-3, interleukin (IL)-6 and C-reactive protein (CRP); and in CSF, including soluble intracellular cell adhesion molecule-1 (sICAM-1), IL-8, 10 kDa interferon-γ-induced protein, and CRP. AD pathology interacted with CSF sICAM-1 in association with NPS. Presenting NPS was associated with subsequent cognitive decline which was mediated by CSF sICAM-1.
Conclusions

Distinct systemic and CNS inflammatory processes are involved in the pathophysiology of NPS in older people. Neuroinflammation may explain the link between NPS and more rapid clinical disease progression.
Effect of Low-Dose Tadalafil Once Daily On Glycemic Control In Patients With Type 2 Diabetes and Erectile Dysfunction: A Randomized, Double-blind, Placebo-controlled Pilot Study
Authors: Lee Min-Kyung , Lee Jae-Hyuk , Sohn Seo-Young , Lee Seo Yeon , Jeong Tae-Yoong , Kim Sae Chul 
Published: November 2021

Abstract
Background: Phosphodiesterase type 5 inhibitors restore nitric oxide signaling, which plays a significant role in erectile function and appears to counteract insulin resistance in animal and human models. This study was aimed to evaluate the glycemic and metabolic effects of low-dose tadalafil once a day in patients with type 2 diabetes and erectile dysfunction.Methods: A 6-month, randomized, double-blind, placebo-controlled pilot trial was conducted. Eligible patients were randomly assigned in a ratio of 2:1 to the tadalafil 5 mg and placebo groups; all patients received either tadalafil or placebo once a day. The primary efficacy endpoint was the change in the glycated hemoglobin (HbA1c) level during the 6-month study period. The secondary efficacy endpoints included metabolic parameters and erectile function. Results: Of the 68 patients who completed this study, 45 and 23 patients were allocated in the tadalafil and placebo groups, respectively. The mean HbA1c level was significantly different between the groups over the 6-month study period (P = 0.021). After 6 months of treatment, the HbA1c decrement in the tadalafil group was greater than that in the placebo group (-0.14% ± 0.53% vs. 0.20% ± 0.69%, P = 0.030). The improvement in the International Index of Erectile Function-5 scores were significantly greater in the tadalafil group than in the placebo group at 6 months (P = 0.003). Conclusion: This prospective pilot study shows that low-dose tadalafil once a day is effective in improving glycemic control and erectile function in patients with type 2 diabetes and erectile dysfunction.Trial Registration: KCT0005666
Synthesis and Characterization of CuO Nanoparticles and CuO/PVA-PVP Nanocomposites: Adsorption Activity for Malachite Green (MG) Dye and Antibacterial Property in Wastewater Treatment
Authors: Ghosh Nikita , Sen Subhadeep , Biswas Goutam , Singh L. Robindro , Haldar Prabir Kumar 
Published: November 2021

Abstract
Contaminated waterways, particularly effluent from the dye industry, are one of the major issues today. Among various methods adsorption being the most straight forward, low-cost, effective, and successful to treat such effluents. For this purpose, Copper oxide nanoparticles (CuO) and Copper oxide nanoparticles embedded within Polyvinyl alcohol (PVA) and Polyvinyl pyrrolidone (PVP) mixed polymer (CuO/PVA-PVP) were synthesized using precipitation methods and different analytical techniques such as dynamic light scattering (DLS), powdered X-ray diffraction (XRD), fourier-transform infrared (FTIR) spectroscopy and transmission electron microscopy (TEM) were used to characterize morphology and size. Synthesized nanomaterials were used for the adsorption of malachite green (MG) dye in aqueous solution by the batch adsorption method. Various experimental conditions e.g., contact time, concentrations, pH, were optimized to obtain best adsorption outcome. Adsorption kinetics and isotherm suggested that it follows Pseudo-second-order-kinetic and Freundlich isotherm. The invitro antibacterial activity was investigated using disk diffusion and minimum inhibitory concentration (MIC) test against Staphylococcus aureus (S.aureus), a gram positive and Escherichia coli (E.coli), a gram negative bacteria, where both the synthesized nanoparticles showed a decent antibacterial activity against both the bacterial strain.
Zhizhu Kuanzhong Capsule in Treating Patients With Functional Dyspepsia Postprandial Distress Syndrome: Study Protocol For a Multicenter, Randomized, Double-blind, Placebo-Controlled, Parallel Group Clinical Trial
Authors: Xiao Mengli , Zhong Linda LD , Lam Wai Ching , Zhao Yingpan , Gwee Kok-Ann , Holtmann Gerald , Tack Jan , Suzuki Hidekazu , Chen Min-Hu , Xiao Yinglian , Hou Xiaohua , Liu Jinsong , Li Yang , Tang Xu-Dong , Lu Fang 
Published: November 2021

Abstract
Background: Functional dyspepsia (FD) is one of the most common functional gastrointestinal disorders. Based on the various symptoms present in patients with functional dyspepsia-postprandial distress syndrome (FD-PDS), routine agents such as acid suppressants, prokinetic drugs, and centrally acting drugs, offer limited treatment choice with potential side effects. As preliminary clinical trial showed that the marketed product Zhizhu Kuanzhong Capsule (ZZKZ) can improve symptoms in FD-PDS patients, our study aims to provide further evidence on the clinical efficacy and safety of ZZKZ in the treatment of patients with FD-PDS. Methods: In this multicenter, randomized, double-blind, placebo-controlled, parallel group clinical trial, we recruit patients with FD-PDS from 18 hospitals in China and Australia. The trial enrolls patients with FD-PDS based on the Rome IV diagnostic criteria. A total of 480 eligible patients are randomized 1:1 into either ZZKZ or placebo group with 8 weeks of treatment and 4 weeks of follow-up. The primary endpoint is measured by self-rated Visual Analogue Score (VAS) for the degree of discomfort with both symptoms of postprandial fullness and early satiation, recorded once a day and 7 days a week. The primary analysis aims to compare the response rate for FD-PDS VAS score between the groups before and after 8 weeks of treatment with an alpha level of 0.05 (2-sided).Discussion: This trial aims to strengthen the evidence for the efficacy and safety of ZZKZ, a marketed product, in treating FD-PDS. Compared to previous clinical trial targeted FD-PDS, this trial has 8-week double-blind treatment period to investigate the effect of long-term mediation through comparison with the placebo group.Trial registration: This study is registered with ClinicalTrials.gov on 28 January 2019, number NCT03825692.
A Bi-Level Model for a Closed-Loop Agricultural Supply Chain Considering Biogas and Compost
Authors: Cheraghalipour Armin , Roghanian Emad 
Published: November 2021

Abstract
In today's competitive marketplace, to increase customer satisfaction and profitability, supply chain management has become more prominent. Therefore, thorough planning and designing the supply chain by seeing all levels and units are essential to growing the efficiency of the entire supply chain. In the present study, an eight-echelon network is designed for a closed-loop agricultural supply chain. These eight echelons are consist of suppliers, farms, distribution centers (DCs), customers, recycling depots, biogas centers, compost production centers, and biogas applicants. To design the agricultural logistics network, a bi-level programming mathematical model is presented that optimizes the network costs and profits. Also, some meta-heuristics and hybrid meta-heuristics are applied for solving the formulated problem. It should be noted that bi-level programming problems are part of the NP-hard class and due to the computational complexity of the problems, the meta-heuristic algorithms are utilized. Finally, various comparisons and analyses are performed to evaluate the model's performance and the capabilities of the solution methods.
Nano Pom-Poms Prepared Exosomes for Highly Specific Cancer Biomarker Detection
Authors: He Nan , Thippabhotla Sirisha , Zhong Cuncong , Greenberg Zachary , Xu Liang , Pessetto Ziyan , Godwin Andrew , Zeng Yong , He Mei 
Published: November 2021

Abstract
Extracellular vesicles (EVs), particularly nano-sized small EV exosomes, are emerging biomarker sources. However, due to heterogeneous populations secreted from diverse cell types, mapping exosome multi-omic molecular information specifically to their pathogenesis origin for cancer biomarker identification is still extraordinarily challenging. Herein, we introduced a novel 3D-structured nanographene immunomagnetic particles (NanoPoms) with unique flower pom-poms morphology and photo-click chemistry for specific marker-defined capture and release of intact exosome. This specific exosome isolation approach leads to the expanded identification of targetable cancer biomarkers with enhanced specificity and sensitivity, as demonstrated by multi-omic exosome analysis of bladder cancer patient tissue fluids using the next generation sequencing of somatic DNA mutations, miRNAs, and the global proteome. The NanoPoms prepared exosomes also exhibit distinctive in vivo biodistribution patterns, highlighting the highly viable and integral quality. The developed method is simple and straightforward, which is applicable to nearly all types of biological fluids and amenable for enrichment, scale up, and high-throughput exosome isolation.
EDUCAÇÃO POPULAR E EDUCADORES SOCIAIS NAS UNIVERSIDADES: A LUTA DOS MOVIMENTOS SOCIAIS
Authors: Paulo Fernanda dos Santos , Da Silva Elenita Lopes 
Published: November 2021

RESUMO
O artigo discute a relação trabalho-formação de educadores sociais que atuam nas periferias urbanas de Porto Alegre - RS. Apresentamos as categorias educador popular e educador social e acerca dos espaços educativos de Educação Não Escolar. As categorias são contextualizadas histórico-politicamente, fazendo uso da pesquisa participante. Além disso, regatamos a importância dos movimentos sociais na perspectiva da Educação Popular, articulando as áreas Educação e Serviço Social.

Palavras-chave: Educação Popular e universidade. Educação Não Escolar. Educadores Sociais.
Deacidification through calcium carbonate dosing in combination with ultrafiltration
Authors: Morales Yair G. , Lipp Pia 
Published: November 2021

Abstract
Soft acidic waters are often treated for drinking water purposes by using limestone filters to attain chemical equilibrium. The present study investigated the process parameters of a relatively new process combination in which powdered calcium carbonate (CaCO3) was added prior to an ultrafiltration (UF). In order to reach the targeted pH value (≥7.8), dosing concentration, type of material and retention time were evaluated in pilot-scale experiments. The deacidification followed the same kinetics as for limestone filtration and yielded similar filtrate characteristics with dosing concentrations of 20 and 40 g/L CaCO3. No significant increase in transmembrane pressure was observed during the operation of a pilot-scale UF module at low flux (34 L m−2 h−1). Critical flux was determined in a laboratory scale to evaluate the potential impact of CaCO3 particles on the UF operation. Stepping-flux experiments revealed the presence of fouling only at high-dosing concentrations, resulting in a critical flux of 55 L m−2 h−1. At a higher flux, a CaCO3-fouling layer was formed, which decreased the membrane's permeability by 20% over 5 h. Considering that effective air-enhanced backwash and acidic chemical cleanings will be implemented in large-scale applications, the investigated process combination promises to be an appropriate treatment technology for turbid and soft acidic waters.
Application of meteorological element combination-driven SWAT model based on meteorological datasets in alpine basin
Authors: Gu Pengfei , Wu Yongxiang , Liu Guodong , Xia Chengcheng , Wang Gaoxu , Xia Jing , Chen Ke , Huang Xiaohua , Li Daiyuan 
Published: November 2021

Abstract
Thus far, reanalysis-based meteorological products have drawn little attention to the influence of meteorological elements of products on hydrological modeling. This study aims to evaluate the hydrological application potential of the precipitation, temperature, and solar radiation of the China Meteorological Assimilation Driving Datasets for the Soil and Water Assessment Tool (SWAT) model (CMADS) and Climate Forecast System Reanalysis (CFSR) in an alpine basin. The precipitation, temperature, and solar radiation of the gauge-observed meteorological dataset (GD), CFSR, and CMADS were cross-combined, and 20 scenarios were constructed to drive the SWAT model. From the comprehensive comparisons of all scenarios, we drew the following conclusions: (1) among the three meteorological elements, precipitation has the greatest impact on the simulation results, and using GD precipitation from sparse stations yielded better performance than CMADS and CFSR; (2) although the SWAT modeling driven by CMADS and CFSR performed poorly, with CMADS underestimation and CFSR overestimation, the temperature and solar radiation of CMADS and CFSR can be an alternative data source for streamflow simulation; (3) models using GD precipitation, CFSR temperature, and CFSR solar radiation as input yielded the best performance in streamflow simulation, suggesting that these data sources can be applied to this data-scarce alpine region.