bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification of Five m6A-Relevant mRNAs Signature and Risk Score for The Prognostication of Gastric Cancer
Authors: Sun Dongwei , Hang Xiaorong , Ren Jun , Sun Qiannan , Liu Zhu , Wang Yong , Wang Daorong 
Published: May 2021

Abstract
Introduction: N6-methyladenosine (m6A) is the most abundant form of methylation modification in eukaryotic cell mRNA. However, the role of m6A in gastric cancer is unclear, which is one of the most common gastrointestinal malignancies. The m6A-relevant mRNA signatures and risk scores are determined to predict the prognosis of gastric cancer in this study.Methods: The expression profiles and clinical information of 367 patients were downloaded from the Cancer Genome. Cluster analysis and univariate Cox analysis were used to identify the regulatory factors of RNA methylation associated with gastric cancer prognosis. The co-expression network was constructed by the WGCNA package in R. Then, the correlations between module eigengenes and clinical traits were calculated to identify the relevant modules. We used univariate Cox analysis to screen for genes that were significantly associated with prognosis in the module (P < 0.01 was considered significant). We identified hub genes by LASSO and multivariate analysis, and developed a Cox prognostic model. Finally, the hub gene expression values weighted by the coefficients from the LASSO regression generated a risk score for each patient, receiver operating characteristic (ROC) and Kaplan-Meier curves were used to assess the prognostic capacity of the risk scores.Results: HNRNPC was shown as P<0.05 and HR>1 in the TCGA gastric cancer data set, which might be a pathogenic factor affecting the prognosis of gastric cancer. The results indicated that AARD, ASPN, SLAMF9, MIR3117 and DUSP1 were hub genes affecting the prognosis of gastric cancer patients, and the m6A methylation of these mRNAs might be regulated by HNRNPC. The risk score = − (0.166195281×AARD + 0.016850602×ASPN + 0.591607997×SLAMF9 + 0.591607997×MIR3117 + 0.00276337×DUSP1), and our results indicated a bad performance of the five-gene signature for survival prediction (P < 0.05).Conclusion: HNRNPC (a m6A RNA methylation regulator) can participate in the serious progression of gastric cancer by regulating the m6A of AARD, ASPN, SLAMF9, MIR3117 and DUSP1, which may be used for prognosis stratification and treatment strategy formulation.
A Plant-Based Meal Reduces Postprandial Oxidative and Dicarbonyl Stress in Men with Diabetes or Obesity more than an Energy- and Macronutrient-Matched Conventional Meal in a Randomized Crossover Study
Authors: Malinska Hana , Klementova Marta , Kudlackova Michaela , Veleba Jiri , Hoskova Eva , Oliyarnyk Olena , Markova Irena , Thieme Lenka , Hill Martin , Pelikanova Terezie , Kahleova Hana 
Published: May 2021

Abstract
Background: Increased oxidative/dicarbonyl stress and chronic inflammation are considered key pathological mediators in the progression of complications in type 2 diabetes (T2D) and obesity. Lifestyle and diet composition have a major impact. In this study, we tested the effects of a vegan (V) and a conventional meat containg (M) meal, matched for energy and macronutrients, on postprandial oxidative and dicarbonyl stress, inflammatory markers and appetite hormones. Methods: A randomised crossover design was used to evaluate T2D, obese and control participants (n=20 in each group), with serum concentrations of analytes determined at 0, 120 and 180 minutes. Repeated-measures ANOVA was used for statistical analysis.Results: Postprandial glucose, triglycerides and free fatty acid responses were similar after both meals. After the V-meal consumption, we observed decreased postprandial concentrations of oxidised glutathione (p˂0.001) and increased glutathione peroxidase activity (p=0.045) compared with the M-meal in T2D subjects. In obese participants, V-meal consumption increased postprandial concentrations of reduced glutathione (p=0.041) and decreased methylglyoxal concentrations (p=0.023). There were no differences in postprandial secretion of TNFa, MCP-1 or ghrelin, but we did observe higher postprandial secretion of leptin after the V-meal in T2D subjects (p=0.002) compared with the M-meal. Conclusions: The results show that a plant-based meal is efficient in ameliorating the postprandial oxidative and dicarbonyl stress compared to a conventional energy- and macronutrient-matched meal, indicating the therapeutic potential of plant-based nutrition in improving the progression of complications in T2D and obese patients.Registered under ClinicalTrials.gov Identifier No. NCT02474147.
Study of Wind Flow Patterns and Heavy Gas Pollutants Dispersion Under Isolated Building Terrain
Authors: Fan Yuyan , Gao Hong 
Published: May 2021

Abstract
Experiment and simulation were used to study the dispersion characteristics of heavy gas pollutants represented by carbon dioxide in isolated building terrain. Wind tunnel experiment and laser particle tracing technology were used to characterize the features of wind flow and the dispersion of pollutants. The influences of the distance from the building to the release source and the size of the building were explored. The results show that the height of the building has a significant effect on the wind speed and turbulence intensity on the windward and leeward sides of the building, and the side width of the building has a slightly weaker effect. The area of the recirculation region on the leeward side of the building and the barrier effect on pollutants are dominated by the windward area of the building, that is, the larger the windward area, the larger the recirculation region and the lower the concentration of pollutants on the leeward side. At the same time, the decline rate of pollutants on the windward side increases with the increase of the windward area. The heavy gas pollutants tend to spread around the building side with the wind flow, unless the distance from the building to the source is too short or the building is too long. And when the pollutants climb to the top of the building, the dispersion of them will slows down as the width of the building side increases. The RNG k-ε model was used for simulation to provide a visible result for wind flow, and its applicability and accuracy were verified.
Comprehensive Evaluation of the Toxicity of the Flame Retardant (decabromodiphenyl ether) in a Bioindicator Fish (Gambusia affinis)
Authors: Iglesias Juan Manuel Pérez , Gonzalez Patricia Silvia , Calderon Mirian Roxana , Natale Guillermo Sebastian , Almeida Cesar Americo 
Published: May 2021

Abstract
In recent years, polybrominated diphenyl ethers or PBDE have been identified as the new emerging pollutants. These pollutants are derived from e-waste and their adverse effect on biota has been proven. In this work, the adverse effects of BDE-209 on mosquitofish (Gambusia affinis) were evaluated. Acute toxicity bioassays were carried out with daily renewal of solutions, using different concentrations of environmental relevance ranged between 10 and 100 µg.L− 1 of BDE-209. After 48 and 96 h of exposure, mortality, individual activity (swimming), biochemical activity (catalase; thiobarbituric acid reactive substances; and acetylcholinesterase), and cytotoxic responses (micronucleus frequencies) were studied. In addition, integrated biomarker response and multivariate analyses were conducted to study the correlation of biomarkers. The calculated lethal concentrations 50 remained constant after all exposure times (24 to 96 h), and the corresponding value was 27.79 µg.L− 1 BDE-209. Furthermore, BDE-209 induced effects on the swimming activity of this species in relation to acetylcholine, since BDE-209 increased, produced oxidative damage at the biochemical level and genotoxicity after 48 h of sublethal concentrations (10 and 25 µg.L− 1 BDE-209) of exposure. The results show that BDE-209 has biochemical, cytotoxic, neurotoxic and genotoxic potential on G. affinis. In addition, mosquitofish can be used as a good bioindicator to evaluate environmental stressors and flame retardants could be a risk factor to Neotropical species.
Adjuvant Immunotherapy Recommendations For Stage III Melanoma: Physician And Nurse Interviews.
Authors: Livingstone Ann , Dempsey Kathy , Stockler Martin R. , Howard Kirsten , Long Georgina V. , Carlino Matteo S. , Menzies Alexander M. , Morton Rachael L. 
Published: May 2021

Abstract
BackgroundAdjuvant immunotherapy is revolutionising care for patients with resected stage III and IV melanoma. However, immunotherapy may be associated with toxicity, making treatment decisions complicated. This study aimed to identify factors physicians and nurses considered regarding adjuvant immunotherapy for melanoma.MethodsIn-depth interviews were conducted with physicians (medical oncologists, surgeons and dermatologists) and nurses managing patients with resected stage III melanoma between July 2019 and March 2020. Factors considered regarding adjuvant immunotherapy were explored. Recruitment continued until data saturation and thematic analysis was undertaken. ResultsTwenty-five physicians and nurses, aged 28-68 years, 60% females, including eleven (44%) medical oncologists, eight (32%) surgeons, five (20%) clinical nurse consultants, and one (4%) dermatologist were interviewed. Over half the sample managed five or more new resected stage III patients per month who could be eligible for adjuvant immunotherapy. Three themes about adjuvant immunotherapy recommendations emerged: (1) clinical and patient factors, (2) treatment information provision, and (3) individual physician/nurse factors. Melanoma sub-stage and an individual patient's therapy risk/benefit profile were primary considerations. Secondary factors included uncertainty about adjuvant immunotherapy's effectiveness and their views about treatment burden patients might consider acceptable.ConclusionsPatients' disease sub-stage and their treatment risk versus benefit drove the melanoma health care professionals' adjuvant immunotherapy endorsement. Findings will aid clinical communication with patients and facilitate clinical decision-making about management options for resected stage III melanoma.
Mechanical Performance Under Various Conditions of 3D Printed Polylactide Composites With Natural Fibers
Authors: Mazur Karolina E. , Borucka Aleksandra , Kaczor Paulina , Gądek Szymon , Kuciel Stanislaw 
Published: May 2021

Abstract
In the study, polylactide-based (PLA) composites modified with natural particles (wood, bamboo, and cork) and with different levels of infilling (100%, 80%, and 60%) obtained by additive methods were tested. The effect of type fiber, infill level and crystallization rate on the mechanical properties were investigated by using tensile, flexural, and impact tests. The materials were subjected to mechanical tests carried out at 23 and 80 °C. Furthermore, hydrothermal degradation was performed, and its effect on the properties was analyzed. The addition of natural fillers and different level of infilling result in a similar level of reduction in the properties. Composites made of PLA are more sensitive to high temperature than to water. The decrease in Young's modulus of PLA at 80 °C was 90%, while after 28 days of hydrodegradation ~ 9%. The addition of fibers reduced this decrease at elevated temperatures. Moreover, the impact strength has been improved by 50% for composites with cork particles and for other lignocellulosic composites remained at the same level as for resin.
Long Non-Coding RNA SNHG19 Promotes Breast Cancer Progression by Regulating miR-299-5p
Authors: Lu Hongquan , Jiang Zhenjia 
Published: May 2021

Abstract
Background: Accumulating evidence has suggested that long noncoding RNA (lncRNA) played crucial roles in the development of human malignances including breast cancer. SNHG19 is a newly identified lncRNA which exerted oncogenic function in non-small cell lung cancer, but whether SNHG19 was involved the development of other cancer, such as breast cancer still unclear. Methods: qRT-PCR was performed to examine the expression of SNHG19 and miR-299-5p in breast cancer tissues and cell lines. Cell proliferation was measure using CCK-8 and colony formation assay. Cell migration and invasion ability was detected by wound healing assay and transwell invasion assay. Bioinformatics analysis, dual luciferase reporter assay, RIP assay and Pull down assay were used to verify the direct binding between SNHG19 and miR-299-5p. The xenotransplantation mouse model was established to explore the effect of SNHG19 on breast cancer tumor growth in vivo.Results: We found that SNHG19 expression level was up-regulated in breast cancer tissues and cell lines, while miR-299-5p expression was down-regulated in breast cancer tissues and it was negatively correlated with SNHG19 expression. Silence of SNHG19 inhibited breast cancer cells proliferation, migration and invasion in vitro. Moreover, SNHG19 knockdown suppressed tumor growth of breast cancer cells in vivo. Mechanistically, SNHG19 acted as a ceRNA (competitive endogenous RNA) to sponge miR-299-5p. Finally, the rescue assays further confirmed that miR-299-5p inhibitor reversed the inhibitory effects of SNHG19 knockdown on breast cancer cell proliferation, migration and invasion.Conclusions: In conclusion, our findings proved that SNHG19 promoted breast cancer progression via sponging miR-299-5p and might function as promising prognostic indicator and therapeutic target for breast cancer.
Profile of Oncological Patients Needing Rehabilitation by Dental and Bucomaxillofacial Prostheses in a Brazilian Subpopulation
Authors: Garcia Cristiane Loreda , Gialain Ivan Onone , Volpato Maria Carmen Palma Faria , Volpato Luiz Evaristo Ricci 
Published: May 2021

Abstract
Purpose: This study aimed to identify the profile of cancer patients in need of rehabilitation with oral and/or buccomaxillofacial prostheses, as well as to evaluate the possible reasons for not concluding the rehabilitation.Materials & Methods: This is a retrospective observational study carried out at the Dentistry Department of the Mato Grosso Cancer Hospital, Cuiabá, MT, Brazil, through the evaluation of the medical records of patients attended from April 2017 to November 2019.Results: The study population comprised 256 patients who met the research inclusion criteria. It was found that 30.90% of the patients were elderly, 65.6% were men, 70.3% brown, 27.3% retired, 49.2% married and 52% coming from municipalities of the interior of the state of Mato Grosso. From the total of patients, 67.23% reported smoking and 53.9% alcohol consumption. As for the location of the tumor, 57.4% had it in the head and neck region, 55.1% of which were epidermoid carcinoma and in 28.9% of cases the disease stage was IV. Most of the patients (60.2%) completed prosthetic rehabilitation, with total prostheses predominating. The main reasons for not completing the rehabilitation were the patient's death and weakness.Conclusions: Patients who started treatment in more advanced stages of cancer had a greater chance of not completing the prosthetic rehabilitation, and the incompletion of rehabilitation treatment was directly related to the patients’ death and the state of weakness.
Design and Parametric Analysis of Wideband Micro-Strip Patch Antenna With CPW-FED
Authors: Tiwari Rahul , BAGWARI ASHISH , kushwah Vivek 
Published: May 2021

Abstract
A design and parametric analysis of patch antennas (MPA) with coplanar waveguide (CPW-Fed) for wideband application. It is analyzed a compact coplanar waveguide patch antenna that has dimensions of 35 mm x 39 mm. Compact Inverted U-slotted-shaped antenna uses the material with a dielectric constant of 4.3 and the height of the antenna is h =1.6 mm. This paper analysis of different parameters of antenna dimensions and designs compact CPW-fed patch antennas for the range from 3.20GHz to 7.94 GHz. The main purpose of the parametric analysis of proposed antennas is to achieve higher impedance bandwidth for wideband applications.
Sub-Ambient Radiative Cooling Realized Using CaCO3 Microparticle-Based Single Layer Without Metal Reflector for Entire Day
Authors: Lim Hangyu , Chae Dongwoo , Son Soomin , Ju Sucheol , Ha Jisung , Lee Heon 
Published: May 2021

Abstract
Conventional cooling systems, that is, air conditioners, should be replaced because they consume a substantial amount of energy and cause environmental pollution. In this context, radiative cooling systems, which perform cooling without consuming any energy or causing environmental pollution, are emerging as an alternative. However, most of the radiative coolers explored thus far include metals, such as silver, that are used as solar reflectors, thereby entailing problems in terms of practicality, mass production, cost, and light pollution. Herein, we propose calcium carbonate (CaCO3) micro-particle-based radiative cooling, which utilizes the high-energy band gap of CaCO3 for high-performance radiative cooling. As the cooler has only a single layer of a CaCO3 composite without any metal reflector, it is mass-producible, cheap, and does not cause light pollution. To demonstrate the cooling performance of CaCO3, optical properties and temperature changes are measured and compared with those of commercial white paint. As a result, it is demonstrated that the CaCO3-based radiative cooler has cooling power 93.1 W/m2 in calculation and can be cooled 6.52 ℃ and 3.38 ℃ under ambient temperature in daytime and nighttime respectively. Thus, it can perform as radiative cooler in entire day.
Absence of Right Pulmonary Artery With Pulmonary Valve Stenosis and Partial Connection of Descending Aorta：A Case Report
Authors: Jiang wenlong , Cheng Jing , Cheng Fangzhou 
Published: May 2021

Abstract
Background:Unilateral absence of pulmonary artery is a rare congenital disorder that can remain asymptomatic until adulthood. Absence of right pulmonary artery (ARPA) has been reported in two-thirds of this patient population.It may be isolated, but it is more often associated with other congenital cardiac defects such as aortic coarctation, tetralogy of Fallot, atrial septal defect and patent ductus arteriosus.Case presentation: We are the first to introduce an adult case of ARPA with pulmonary valve stenosis（PVS） and associated with partial connection of descending aorta.We investigated the condition by right heart catheterization,right ventricle angiography, pulmonary arteriography and aorta angiography. According to the above results，we suggest that surgical treatment should be positively consideredConclusions: The case remind that echocardiography combined with detailed imaging examination is very important for the diagnosis of complex congenital heart disease and provide the basis for the selection of the best treatment.
Morphological, Cytochemical and Ultrastructural Aspects of Blood Cells in Freshwater Stingray Species in the Middle Rio Negro Basin, Amazonian, Brazil
Authors: de Oliveira Adriano Teixeira , Lemos Jefferson Raphael Gonzaga , Santos Marcio Quara Carvalho , Pantoja-Lima Jackson , Aride Paulo Henrique Rocha , Araújo Maria Lúcia Góes , Tavares-Dias Marcos , Marcon Jaydione Luiz 
Published: May 2021

Abstract
Examined the morphology, dimensions, cytochemical staining reactions and ultrastructure of blood cells from three freshwater stingray species, Potamotrygon wallacei, Potamotrygon motoro and Paratrygon aiereba, living in the waters of the middle Rio Negro basin (Barcelos, Amazonas, Brazil). We identified erythrocytes, erythroblasts, thrombocytes and four types of leukocyte (basophils, heterophils, lymphocytes and monocytes) in the blood of these stingray species. In all the freshwater stingrays studied, the shape and dimensions of these cells were similar to those of marine elasmobranchs. A positive PAS reaction occurred in heterophils and thrombocytes, and a weak reaction in lymphocytes and monocytes, while a metachromasia reaction only occurred in basophils. Sudan black staining was positive for thrombocytes and lymphocytes, and only a weak reaction occurred in heterophils. Basophils and heterophils were the only cells stained with bromophenol blue, while no peroxidase reaction was observed in any leukocyte type. This is the first study to establish the dimensions and cytochemical staining reactions of blood cells in Amazonian stingray species. Since these elasmobranch species are exported as ornamental fish to countries worldwide, this study can contribute towards establishing standards for blood constituents that may be helpful in assessing the health and welfare of these fish in artificial systems.
Interfacial Quality Prediction Model for Al/Steel Sheets During Friction Stir Assisted Double-Sided Incremental Forming with Synchronous Bonding
Authors: Wu Renhao , Li Meng , Liu Xinmei , Yang Zhiyun , Chen Jun 
Published: May 2021

Abstract
The widely investigated Al/steel laminated structures are challenged with subsequent plastic deformation due to the existence of interfacial brittle intermetallic compound layer. To overcome this drawback, a newly proposed thermomechanical forming technology as friction stir assisted double-sided incremental plastic forming with synchronous solid-state interfacial bonding is utilized to fabricate laminated structures, which can meet requirement of plastic deformation of laminates. Typical interfacial bonding performances produced by a series of experiments classified as sound bonding, de-bonding, over-thinning, penetration and crack are assessed. Local working peak temperature and maximum forming force in loading area are recorded and evaluated during stable bonding-forming stage. Considering heat-force coupling effect, a pressure-strain-temperature based prediction model is modified to assess process quality, which is conformed to experimental results. This work can help obtain proper process window to fabricate Al/steel laminated parts and shall also inspire to build guidance of related thermomechanical joining-with-forming processes to achieve high interfacial performance.