bluuebunny/crossref_metadata_2025_split huggingface dataset:
ENSO Modulates Summer and Autumn Sea Ice Variability Around Dronning Maud Land, Antarctica
Authors: Isaacs Florence E. , Renwick James A. , Mackintosh Andrew N. , Dadic Ruzica 
Published: February 2021

AbstractAntarctica’s sea ice cover is an important component of the global climate system, yet the drivers of sea ice variability are not well understood. Here we investigated the effects of climate variability on sea ice concentration (SIC) around East Antarctica by correlating the 40‐years (1979–2018) satellite sea ice record and ERA5 reanalysis data. We found that summer and autumn SIC around Dronning Maud Land (DML) between 10 and 70°E exhibited a statistically significant negative correlation with the Niño 3.4 index. Sea ice in DML was also correlated with sea surface temperature (SST) anomalies in the tropical Pacific, and to an atmospheric wave train pattern extending from the South Pacific to DML. We suggest that a southward‐propagating atmospheric wave train triggered by SST anomalies in the tropical Pacific extends into DML and alters sea ice concentration by encouraging meridional airflow. Our results showed that shifts in meridional flow in DML affected sea ice thermodynamically, by altering local heat transport and in turn altering sea ice formation and melt.
Meteorological Environments Associated With California Wildfires and Their Potential Roles in Wildfire Changes During 1984–2017
Authors: Dong Lu , Leung L. Ruby , Qian Yun , Zou Yufei , Song Fengfei , Chen Xiaodong 
Published: February 2021

AbstractCalifornia has experienced more wildfires in recent years, resulting in huge economic losses and threatening human health. Clarifying the meteorological environments of wildfires is foundational to improving the understanding and prediction of wildfires and their impacts. Here, 1,535 California wildfires during 1984–2017 are systematically investigated. Based on two key meteorological factors—temperature and moisture anomalies—all wildfires are classified into four groups: hot‐dry, hot‐wet, cold‐dry, cold‐wet. Most (∼60%) wildfires occurred on hot‐dry days. Compositing the meteorological environments of the four groups shows that persistent high pressure and strong northeasterly wind descending from inland favor hot‐dry conditions for wildfires. Self‐organizing map analysis lends confidence in the large‐scale meteorological pattern dominating hot‐dry wildfires in California. Meteorological anomalies also influence wildfire size through their magnitudes, with moisture anomaly explaining the largest fraction (∼69%) of variability in wildfire sizes. In addition, 12.2% of wildfires occurred on hot‐wet days, which may be related with lightning flashes. More lightning tends to trigger wildfires, but the wet condition helps to suppress the wildfire sizes. Total burned area by wildfires has significantly increased by ∼3.6% per year, indicating a doubling of burned area in 2017 relative to 1984, mainly dominated by hot‐dry wildfires in summer. Drying and warming in conjunction with strengthening of the high pressure in summer and fall have the potential to support more frequent and larger hot‐dry wildfires in California during the past several decades.
High‐Frequency Seismic Events on Mars Observed by InSight
Authors: van Driel Martin , Ceylan Savas , Clinton John F. , Giardini Domenico , Horleston Anna , Margerin Ludovic , Stähler Simon C. , Böse Maren , Charalambous Constantinos , Kawamura Taichi , Khan Amir , Orhand‐Mainsant Guenolé , Scholz John‐R. , Euchner Fabian , Knapmeyer Martin , Schmerr Nicholas , Pike William T. , Lognonné Philippe , Banerdt William B. 
Published: February 2021

AbstractThe seismometer deployed on the surface of Mars as part of the InSight mission (Interior Exploration using Seismic Investigations, Geodesy and Heat Transport) has recorded several hundreds of marsquakes in the first 478 sols after landing. The majority of these are classified as high‐frequency (HF) events in the frequency range from approximately 1 to 10 Hz on Mars' surface. All the HF events excite a resonance around 2.4 Hz and show two distinct but broad arrivals of seismic energy that are separated by up to 450 s. Based on the frequency content and vertical‐to‐horizontal energy ratio, the HF event family has been subdivided into three event types, two of which we show to be identical and only appear separated due to the signal‐to‐noise ratio. We show here that the envelope shape of the HF events is explained by guided Pg and Sg phases in the Martian crust using simple layered models with scattering. Furthermore, the relative travel times between these two arrivals can be related to the epicentral distance, which shows distinct clustering. The rate at which HF events are observed varies by an order of magnitude over the course of one year and cannot be explained by changes of the background noise only. The HF content and the absence of additional seismic phases constrain crustal attenuation and layering, and the coda shape constrains the diffusivity in the uppermost shallow layers of Mars.
Removal of nonimpacted third molars alters the periodontal condition of their neighbors clinically, immunologically, and microbiologically
Authors: Tian Yi , Sun Lijuan , Qu Honglei , Yang Yang , Chen Faming 
Published: February 2021

AbstractConsidering the adverse effects of nonimpacted third molars (N-M3s) on the periodontal health of adjacent second molars (M2s), the removal of N-M3s may be beneficial to the periodontal health of their neighbors. This study aimed to investigate the clinical, immunological, and microbiological changes of the periodontal condition around M2s following removal of neighboring N-M3s across a 6-month period. Subjects with at least one quadrant containing an intact first molar (M1), M2, and N-M3 were screened and those who met the inclusion criteria and decided to receive N-M3 extraction were recruited in the following investigation. M2 periodontal condition was interrogated before M3 extraction (baseline) and at 3 and 6 months postoperatively. Improvements in clinical periodontal indexes of M2s in response to their adjacent N-M3 removal, along with changes in inflammatory biomarkers among gingival crevicular fluid (GCF) and the composition of subgingival plaque collected from the distal sites of the M2s of the targeted quadrant were parallelly analyzed. Complete data of 26 tooth extraction patients across the follow-up period were successfully obtained and subsequently applied for statistical analysis. Compared to the baseline, the periodontal condition of M2s was significantly changed 6 months after N-M3 removal; specifically, the probing depth of M2s significantly reduced (P < 0.001), the matrix metalloproteinase (MMP)-8 concentration involved in GCF significantly decreased (P = 0.025), and the abundance of the pathogenic generaunidentified PrevotellaceaeandStreptococcussignificantly decreased (P < 0.001 andP = 0.009, respectively). We concluded that N-M3 removal was associated with superior clinical indexes, decreased GCF inflammatory biomarkers, and reduced pathogenic microbiome distribution within the subgingival plaque. Although the retention or removal of N-M3s continues to be controversial, our findings provide additional evidence that medical decisions should be made as early as possible or at least before the neighboring teeth are irretrievably damaged.
Green revolution in electronic displays expected to ease energy and health crises
Authors: Wang Yuyang , Nie Hui , Han Jinsong , An Yaxun , Zhang Yu-Mo , Zhang Sean Xiao-An 
Published: February 2021

AbstractThe technological revolution of long-awaited energy-saving and vision-friendly displays represented by bistable display technology is coming. Here we discuss methods, challenges, and opportunities for implementing bistable displays in terms of molecular design, device structure, further expansion, and required criteria, hopefully benefiting the light-related community.
Inhibition of cystathionine β-synthase promotes apoptosis and reduces cell proliferation in chronic myeloid leukemia
Authors: Wang Dan , Yang Huan , Zhang Yun , Hu Rong , Hu Dongjie , Wang Qunxian , Liu Yannan , Liu Mingjing , Meng Zijun , Zhou Weihui , Song Weihong 
Published: February 2021

AbstractIncreased endogenous hydrogen sulfide (H2S) level by cystathionine β-synthase (CBS) has been shown to closely relate tumorigenesis. H2S promotes angiogenesis, stimulates bioenergy metabolism and inhibits selective phosphatases. However, the role of CBS and H2S in chronic myeloid leukemia (CML) remains elusive. In this study, we found that CBS and H2S levels were increased in the bone marrow mononuclear cells of pediatric CML patients, as well as in the CML-derived K562 cells and CBS expression levels were correlated with different disease phases. Inhibition of CBS reduced the proliferation of the CML primary bone marrow mononuclear cells and induced growth inhibition, apoptosis, cell cycle arrest, and migration suppression in K562 cells and tumor xenografts. The knockdown of CBS expression by shRNA and inhibiting CBS activity by AOAA decreased the endogenous H2S levels, promoted mitochondrial-related apoptosis and inhibited the NF-κB-mediated gene expression. Our study suggests that inhibition of CBS induces cell apoptosis, as well as limits cell proliferation and migration, a potential target for the treatment of chronic myeloid leukemia.
The mTORC1 complex in pre-osteoblasts regulates whole-body energy metabolism independently of osteocalcin
Authors: Tangseefa Pawanrat , Martin Sally K. , Chin Peck Yin , Breen James , Mah Chui Yan , Baldock Paul A. , Wittert Gary A. , Page Amanda J. , Proud Christopher G. , Fitter Stephen , Zannettino Andrew C. W. 
Published: February 2021

AbstractOvernutrition causes hyperactivation of mTORC1-dependent negative feedback loops leading to the downregulation of insulin signaling and development of insulin resistance. In osteoblasts (OBs), insulin signaling plays a crucial role in the control of systemic glucose homeostasis. We utilized mice with conditional deletion of Rptor to investigate how the loss of mTORC1 function in OB affects glucose metabolism under normal and overnutrition dietary states. Compared to the controls, chow-fed Rptorob−/− mice had substantially less fat mass and exhibited adipocyte hyperplasia. Remarkably, upon feeding with high-fat diet, mice with pre- and post-natal deletion of Rptor in OBs were protected from diet-induced obesity and exhibited improved glucose metabolism with lower fasting glucose and insulin levels, increased glucose tolerance and insulin sensitivity. This leanness and resistance to weight gain was not attributable to changes in food intake, physical activity or lipid absorption but instead was due to increased energy expenditure and greater whole-body substrate flexibility. RNA-seq revealed an increase in glycolysis and skeletal insulin signaling pathways, which correlated with the potentiation of insulin signaling and increased insulin-dependent glucose uptake in Rptor-knockout osteoblasts. Collectively, these findings point to a critical role for the mTORC1 complex in the skeletal regulation of whole-body glucose metabolism and the skeletal development of insulin resistance.
USP9X-mediated KDM4C deubiquitination promotes lung cancer radioresistance by epigenetically inducing TGF-β2 transcription
Authors: Jie Xiaohua , Fong William Pat , Zhou Rui , Zhao Ye , Zhao Yingchao , Meng Rui , Zhang Sheng , Dong Xiaorong , Zhang Tao , Yang Kunyu , Wu Gang , Xu Shuangbing 
Published: February 2021

AbstractRadioresistance is regarded as the main barrier to effective radiotherapy in lung cancer. However, the underlying mechanisms of radioresistance remain elusive. Here, we show that lysine-specific demethylase 4C (KDM4C) is overexpressed and correlated with poor prognosis in lung cancer patients. We provide evidence that genetical or pharmacological inhibition of KDM4C impairs tumorigenesis and radioresistance in lung cancer in vitro and in vivo. Moreover, we uncover that KDM4C upregulates TGF-β2 expression by directly reducing H3K9me3 level at the TGF-β2 promoter and then activates Smad/ATM/Chk2 signaling to confer radioresistance in lung cancer. Using tandem affinity purification technology, we further identify deubiquitinase USP9X as a critical binding partner that deubiquitinates and stabilizes KDM4C. More importantly, depletion of USP9X impairs TGF-β2/Smad signaling and radioresistance by destabilizing KDM4C in lung cancer cells. Thus, our findings demonstrate that USP9X-mediated KDM4C deubiquitination activates TGF-β2/Smad signaling to promote radioresistance, suggesting that targeting KDM4C may be a promising radiosensitization strategy in the treatment of lung cancer.
Dynamically remodeled hepatic extracellular matrix predicts prognosis of early-stage cirrhosis
Authors: Wu Yuexin , Cao Yuyan , Xu Keren , Zhu Yue , Qiao Yuemei , Wu Yanjun , Chen Jianfeng , Li Chen , Zeng Rong , Ge Gaoxiang 
Published: February 2021

AbstractLiver cirrhosis remains major health problem. Despite the progress in diagnosis of asymptomatic early-stage cirrhosis, prognostic biomarkers are needed to identify cirrhotic patients at high risk developing advanced stage disease. Liver cirrhosis is the result of deregulated wound healing and is featured by aberrant extracellular matrix (ECM) remodeling. However, it is not comprehensively understood how ECM is dynamically remodeled in the progressive development of liver cirrhosis. It is yet unknown whether ECM signature is of predictive value in determining prognosis of early-stage liver cirrhosis. In this study, we systematically analyzed proteomics of decellularized hepatic matrix and identified four unique clusters of ECM proteins at tissue damage/inflammation, transitional ECM remodeling or fibrogenesis stage in carbon tetrachloride-induced liver fibrosis. In particular, basement membrane (BM) was heavily deposited at the fibrogenesis stage. BM component minor type IV collagen α5 chain expression was increased in activated hepatic stellate cells. Knockout of minor type IV collagen α5 chain ameliorated liver fibrosis by hampering hepatic stellate cell activation and promoting hepatocyte proliferation. ECM signatures were differentially enriched in the biopsies of good and poor prognosis early-stage liver cirrhosis patients. Clusters of ECM proteins responsible for homeostatic remodeling and tissue fibrogenesis, as well as basement membrane signature were significantly associated with disease progression and patient survival. In particular, a 14-gene signature consisting of basement membrane proteins is potent in predicting disease progression and patient survival. Thus, the ECM signatures are potential prognostic biomarkers to identify cirrhotic patients at high risk developing advanced stage disease.
Listeners’ perceptions of the certainty and honesty of a speaker are associated with a common prosodic signature
Authors: Goupil Louise , Ponsot Emmanuel , Richardson Daniel , Reyes Gabriel , Aucouturier Jean-Julien 
Published: February 2021

AbstractThe success of human cooperation crucially depends on mechanisms enabling individuals to detect unreliability in their conspecifics. Yet, how such epistemic vigilance is achieved from naturalistic sensory inputs remains unclear. Here we show that listeners’ perceptions of the certainty and honesty of other speakers from their speech are based on a common prosodic signature. Using a data-driven method, we separately decode the prosodic features driving listeners’ perceptions of a speaker’s certainty and honesty across pitch, duration and loudness. We find that these two kinds of judgments rely on a common prosodic signature that is perceived independently from individuals’ conceptual knowledge and native language. Finally, we show that listeners extract this prosodic signature automatically, and that this impacts the way they memorize spoken words. These findings shed light on a unique auditory adaptation that enables human listeners to quickly detect and react to unreliability during linguistic interactions.
Droplet printing reveals the importance of micron-scale structure for bacterial ecology
Authors: Krishna Kumar Ravinash , Meiller-Legrand Thomas A. , Alcinesio Alessandro , Gonzalez Diego , Mavridou Despoina A. I. , Meacock Oliver J. , Smith William P. J. , Zhou Linna , Kim Wook , Pulcu Gökçe Su , Bayley Hagan , Foster Kevin R. 
Published: February 2021

AbstractBacteria often live in diverse communities where the spatial arrangement of strains and species is considered critical for their ecology. However, a test of this hypothesis requires manipulation at the fine scales at which spatial structure naturally occurs. Here we develop a droplet-based printing method to arrange bacterial genotypes across a sub-millimetre array. We print strains of the gut bacterium Escherichia coli that naturally compete with one another using protein toxins. Our experiments reveal that toxin-producing strains largely eliminate susceptible non-producers when genotypes are well-mixed. However, printing strains side-by-side creates an ecological refuge where susceptible strains can persist in large numbers. Moving to competitions between toxin producers reveals that spatial structure can make the difference between one strain winning and mutual destruction. Finally, we print different potential barriers between competing strains to understand how ecological refuges form, which shows that cells closest to a toxin producer mop up the toxin and protect their clonemates. Our work provides a method to generate customised bacterial communities with defined spatial distributions, and reveals that micron-scale changes in these distributions can drive major shifts in ecology.
Single-molecule force spectroscopy reveals the dynamic strength of the hair-cell tip-link connection
Authors: Mulhall Eric M. , Ward Andrew , Yang Darren , Koussa Mounir A. , Corey David P. , Wong Wesley P. 
Published: February 2021

AbstractThe conversion of auditory and vestibular stimuli into electrical signals is initiated by force transmitted to a mechanotransduction channel through the tip link, a double stranded protein filament held together by two adhesion bonds in the middle. Although thought to form a relatively static structure, the dynamics of the tip-link connection has not been measured. Here, we biophysically characterize the strength of the tip-link connection at single-molecule resolution. We show that a single tip-link bond is more mechanically stable relative to classic cadherins, and our data indicate that the double stranded tip-link connection is stabilized by single strand rebinding facilitated by strong cis-dimerization domains. The measured lifetime of seconds suggests the tip-link is far more dynamic than previously thought. We also show how Ca2+ alters tip-link lifetime through elastic modulation and reveal the mechanical phenotype of a hereditary deafness mutation. Together, these data show how the tip link is likely to function during mechanical stimuli.
Sequence-encoded and composition-dependent protein-RNA interactions control multiphasic condensate morphologies
Authors: Kaur Taranpreet , Raju Muralikrishna , Alshareedah Ibraheem , Davis Richoo B. , Potoyan Davit A. , Banerjee Priya R. 
Published: February 2021

AbstractMultivalent protein-protein and protein-RNA interactions are the drivers of biological phase separation. Biomolecular condensates typically contain a dense network of multiple proteins and RNAs, and their competing molecular interactions play key roles in regulating the condensate composition and structure. Employing a ternary system comprising of a prion-like polypeptide (PLP), arginine-rich polypeptide (RRP), and RNA, we show that competition between the PLP and RNA for a single shared partner, the RRP, leads to RNA-induced demixing of PLP-RRP condensates into stable coexisting phases—homotypic PLP condensates and heterotypic RRP-RNA condensates. The morphology of these biphasic condensates (non-engulfing/ partial engulfing/ complete engulfing) is determined by the RNA-to-RRP stoichiometry and the hierarchy of intermolecular interactions, providing a glimpse of the broad range of multiphasic patterns that are accessible to these condensates. Our findings provide a minimal set of physical rules that govern the composition and spatial organization of multicomponent and multiphasic biomolecular condensates.
Effects of individual base-pairs on in vivo target search and destruction kinetics of bacterial small RNA
Authors: Poddar Anustup , Azam Muhammad S. , Kayikcioglu Tunc , Bobrovskyy Maksym , Zhang Jichuan , Ma Xiangqian , Labhsetwar Piyush , Fei Jingyi , Singh Digvijay , Luthey-Schulten Zaida , Vanderpool Carin K. , Ha Taekjip 
Published: February 2021

AbstractBase-pairing interactions mediate many intermolecular target recognition events. Even a single base-pair mismatch can cause a substantial difference in activity but how such changes influence the target search kinetics in vivo is unknown. Here, we use high-throughput sequencing and quantitative super-resolution imaging to probe the mutants of bacterial small RNA, SgrS, and their regulation of ptsG mRNA target. Mutations that disrupt binding of a chaperone protein, Hfq, and are distal to the mRNA annealing region still decrease the rate of target association, kon, and increase the dissociation rate, koff, showing that Hfq directly facilitates sRNA–mRNA annealing in vivo. Single base-pair mismatches in the annealing region reduce kon by 24–31% and increase koff by 14–25%, extending the time it takes to find and destroy the target by about a third. The effects of disrupting contiguous base-pairing are much more modest than that expected from thermodynamics, suggesting that Hfq buffers base-pair disruptions.
Individualized interactomes for network-based precision medicine in hypertrophic cardiomyopathy with implications for other clinical pathophenotypes
Authors: Maron Bradley A. , Wang Rui-Sheng , Shevtsov Sergei , Drakos Stavros G. , Arons Elena , Wever-Pinzon Omar , Huggins Gordon S. , Samokhin Andriy O. , Oldham William M. , Aguib Yasmine , Yacoub Magdi H. , Rowin Ethan J. , Maron Barry J. , Maron Martin S. , Loscalzo Joseph 
Published: February 2021

AbstractProgress in precision medicine is limited by insufficient knowledge of transcriptomic or proteomic features in involved tissues that define pathobiological differences between patients. Here, myectomy tissue from patients with obstructive hypertrophic cardiomyopathy and heart failure is analyzed using RNA-Seq, and the results are used to develop individualized protein-protein interaction networks. From this approach, hypertrophic cardiomyopathy is distinguished from dilated cardiomyopathy based on the protein-protein interaction network pattern. Within the hypertrophic cardiomyopathy cohort, the patient-specific networks are variable in complexity, and enriched for 30 endophenotypes. The cardiac Janus kinase 2-Signal Transducer and Activator of Transcription 3-collagen 4A2 (JAK2-STAT3-COL4A2) expression profile informed by the networks was able to discriminate two hypertrophic cardiomyopathy patients with extreme fibrosis phenotypes. Patient-specific network features also associate with other important hypertrophic cardiomyopathy clinical phenotypes. These proof-of-concept findings introduce personalized protein-protein interaction networks (reticulotypes) for characterizing patient-specific pathobiology, thereby offering a direct strategy for advancing precision medicine.