bluuebunny/crossref_metadata_2025_split huggingface dataset:
The role of chest CT quantitative pulmonary inflammatory index in the evaluation of the course and treatment outcome of COVID-19 pneumonia
Authors: Peng Song , Chen Jinqing , Zhang Wendy , Zhang Bangjun , Liu Zhifeng , Liu Lang , Wu Zhaofeng , Fu Rui , Li Xiuhua , Lv Fajin 
Published: April 2021

AbstractTo explore the clinical application value of chest CT quantitative pulmonary inflammation index (PII) in the evaluation of the course and treatment outcome of COVID-19 pneumonia. One hundred and eighteen patients with COVID-19 pneumonia diagnosed by RT-PCR were analyzed retrospectively. The correlation between chest CT PII, clinical symptoms and laboratory examinations during the entire hospitalization period was compared. The average age of the patients was 46.0 ± 15 (range: 1–74) years. Of the 118 patients, 62 are male (52.5%) and 56 are female (47.5%). Among them, 116 patients recovered and were discharged, 2 patients died, and the median length of hospital stay was 22 (range: 9–41) days. On admission, 76.3% of the patients presented with fever, and the laboratory studies showed a decrease in lymphocyte (LYM) count and an increase in lactate dehydrogenase (LDH) levels, C-reactive protein (CRP) levels, and erythrocyte sedimentation rate (ESR). Within the studies’ chest CTs, the median number of involved lung lobes was 4 (range: 0–5) and the median number of involved lung segments was 9 (range 0–20). The left lower lobe and the right lower lobe were the most likely areas to be involved (89.0% and 83.9%), and 84.7% of the patients had inflammatory changes in both lungs. The main manifestations on chest CT were ground glass opacities (31.4%), ground glass opacities and consolidation (20.3%), ground glass opacities and reticular patterns (32.2%), mixed type (13.6%), and white lungs (1.7%); common accompanying signs included linear opacities (55.9%), air bronchograms (46.6%), thick small vessel shadows (36.4%), and pleural hypertrophy (13.6%). The chest CT at discharge showed complete absorption of lesions in 19 cases (16.1%), but not in the remaining 99 cases. Lesions remained in a median of 3 lung lobes (range: 0–5). Residual lesions remained in a median of 5 lung segments (range: 0–20). The residual lesions mainly presented as ground glass opacities (61.0%), and the main accompanying sign was linear opacities (59.3%). Based on chest CT, the median maximum PII of lungs was 30.0% (range: 0–97.5%), and the median PII after discharge in the patients excluding the two deaths was 12.5% (range: 0–53.0%). PII was significantly negatively correlated with the LYM count and significantly positively correlated with body temperature, LDH, CRP, and ESR. There was no significant correlation between the PII and the white blood cell count, but the grade of PII correlated well with the clinical classification. PII can be used to monitor the severity and the treatment outcome of COVID-19 pneumonia, provide help for clinical classification, assist in treatment plan adjustments and aid assessments for discharge.
Whole genome resequencing and custom genotyping unveil clonal lineages in ‘Malbec’ grapevines (Vitis vinifera L.)
Authors: Calderón Luciano , Mauri Nuria , Muñoz Claudio , Carbonell-Bejerano Pablo , Bree Laura , Bergamin Daniel , Sola Cristobal , Gomez-Talquenca Sebastian , Royo Carolina , Ibáñez Javier , Martínez-Zapater José Miguel , Lijavetzky Diego 
Published: April 2021

AbstractGrapevine cultivars are clonally propagated to preserve their varietal attributes. However, genetic variations accumulate due to the occurrence of somatic mutations. This process is anthropically influenced through plant transportation, clonal propagation and selection. Malbec is a cultivar that is well-appreciated for the elaboration of red wine. It originated in Southwestern France and was introduced in Argentina during the 1850s. In order to study the clonal genetic diversity of Malbec grapevines, we generated whole-genome resequencing data for four accessions with different clonal propagation records. A stringent variant calling procedure was established to identify reliable polymorphisms among the analyzed accessions. The latter procedure retrieved 941 single nucleotide variants (SNVs). A reduced set of the detected SNVs was corroborated through Sanger sequencing, and employed to custom-design a genotyping experiment. We successfully genotyped 214 Malbec accessions using 41 SNVs, and identified 14 genotypes that clustered in two genetically divergent clonal lineages. These lineages were associated with the time span of clonal propagation of the analyzed accessions in Argentina and Europe. Our results show the usefulness of this approach for the study of the scarce intra-cultivar genetic diversity in grapevines. We also provide evidence on how human actions might have driven the accumulation of different somatic mutations, ultimately shaping the Malbec genetic diversity pattern.
A comparative study of Helicobacter pylori infection in hamsters experimentally infected with liver flukes Opisthorchis felineus, Opisthorchis viverrini, or Clonorchis sinensis
Authors: Pakharukova Maria Y. , Zaparina Oxana , Hong Sung-Jong , Sripa Banchob , Mordvinov Viatcheslav A. 
Published: April 2021

AbstractHelicobacter pylori causes a wide range of human diseases including cancer. Carcinogenic foodborne trematodes Opisthorchis viverrini, Clonorchis sinensis, and O. felineus might promote transmission and spread of H. pylori infection in the definitive mammalian host, which in turn might contribute to the liver fluke-associated malignancy. Our objectives were to find out whether liver flukes O. felineus, O. viverrini, and C. sinensis are carriers of Helicobacter pylori and to determine whether H. pylori is present in feces, bile, and stomach samples from the experimentally infected hamsters. We found that liver flukes are not reservoirs of H. pylori. Nevertheless, the prevalence of H. pylori and the H. pylori ureA gene copy number were significantly elevated after the infection. Overall, although the liver flukes O. felineus, C. sinensis, and O. viverrini are not reservoirs of H. pylori, the infection with the liver flukes significantly modifies the biliary and gut microbiota by increasing H. pylori abundance. This may be a feature of any liver fluke pathogenesis that have not previously been taken into account. Our findings appear to be novel in terms of comparative assessment of the host microbiota and Helicobacter abundance during epidemiologically important liver fluke infections.
Identification of chlorophyll a-b binding protein AB96 as a novel TGFβ1 neutralizing agent
Authors: Lynham Steven , Freile Fabio Grundland , Puri Natasha M. , O’Reilly Nicola , Mitchell Graham H. , Wells Timothy N. C. , Willcox Merlin , Beatson Richard 
Published: April 2021

AbstractThe discovery of compounds and proteins from plants has greatly contributed to modern medicine. Vernonia amygdalina Del. (Compositae) is used by humans and primates for a variety of conditions including parasitic infection. This paper describes the serendipitous discovery that V. amygdalina extract was able to bind to, and functionally inhibit, active TGFβ1. The binding agent was isolated and identified as chlorophyll a-b binding protein AB96. Given that active TGFβ1 contributes to the pathology of many infectious diseases, inhibiting these processes may explain some of the benefits associated with the ingestion of this species. This is the first plant-derived cytokine-neutralizing protein to be described and paves the way for further such discoveries.
Intranasal versus intratracheal exposure to lipopolysaccharides in a murine model of acute respiratory distress syndrome
Authors: Khadangi Fatemeh , Forgues Anne-Sophie , Tremblay-Pitre Sophie , Dufour-Mailhot Alexis , Henry Cyndi , Boucher Magali , Beaulieu Marie-Josée , Morissette Mathieu , Fereydoonzad Liah , Brunet David , Robichaud Annette , Bossé Ynuk 
Published: April 2021

AbstractDue to frequent and often severe lung affections caused by COVID-19, murine models of acute respiratory distress syndrome (ARDS) are increasingly used in experimental lung research. The one induced by a single lipopolysaccharide (LPS) exposure is practical. However, whether it is preferable to administer LPS intranasally or intratracheally remains an open question. Herein, female C57Bl/6 J mice were exposed intranasally or intratracheally to one dose of either saline or 3 mg/kg of LPS. They were studied 24 h later. The groups treated with LPS, either intranasally or intratracheally, exhibited a pronounced neutrophilic inflammation, signs of lung tissue damage and protein extravasation into the alveoli, and mild lung dysfunction. The magnitude of the response was generally not different between groups exposed intranasally versus intratracheally. However, the variability of some the responses was smaller in the LPS-treated groups exposed intranasally versus intratracheally. Notably, the saline-treated mice exposed intratracheally demonstrated a mild neutrophilic inflammation and alterations of the airway epithelium. We conclude that an intranasal exposure is as effective as an intratracheal exposure in a murine model of ARDS induced by LPS. Additionally, the groups exposed intranasally demonstrated less variability in the responses to LPS and less complications associated with the sham procedure.
Machine learning applied to X-ray tomography as a new tool to analyze the voids in RRP Nb3Sn wires
Authors: Bagni T. , Bovone G. , Rack A. , Mauro D. , Barth C. , Matera D. , Buta F. , Senatore C. 
Published: April 2021

AbstractThe electro-mechanical and electro-thermal properties of high-performance Restacked-Rod-Process (RRP) Nb3Sn wires are key factors in the realization of compact magnets above 15 T for the future particle physics experiments. Combining X-ray micro-tomography with unsupervised machine learning algorithm, we provide a new tool capable to study the internal features of RRP wires and unlock different approaches to enhance their performances. Such tool is ideal to characterize the distribution and morphology of the voids that are generated during the heat treatment necessary to form the Nb3Sn superconducting phase. Two different types of voids can be detected in this type of wires: one inside the copper matrix and the other inside the Nb3Sn sub-elements. The former type can be related to Sn leaking from sub-elements to the copper matrix which leads to poor electro-thermal stability of the whole wire. The second type is detrimental for the electro-mechanical performance of the wires as superconducting wires experience large electromagnetic stresses in high field and high current conditions. We analyze these aspects thoroughly and discuss the potential of the X-ray tomography analysis tool to help modeling and predicting electro-mechanical and electro-thermal behavior of RRP wires and optimize their design.
On the shape of cicada’s wing leading-edge cross section
Authors: Starkweather Rachel M. , Poroseva Svetlana V. , Hanson David T. 
Published: April 2021

AbstractAn important role that the leading-edge cross-section shape plays in the wing flight performance is well known in aeronautics. However, little is known about the shape of the leading-edge cross section of an insect’s wing and its contribution to remarkable qualities of insect flight. In this paper, we reveal, in the first time, the shape of the leading-edge cross section of a cicada’s wing and analyze its variability along the wing. We also identify and quantify similarities in characteristic dimensions of this shape in the wings of three different cicada species.
Identification and characterization of piperine synthase from black pepper, Piper nigrum L.
Authors: Schnabel Arianne , Athmer Benedikt , Manke Kerstin , Schumacher Frank , Cotinguiba Fernando , Vogt Thomas 
Published: April 2021

AbstractBlack pepper (Piper nigrum L.) is the world’s most popular spice and is also used as an ingredient in traditional medicine. Its pungent perception is due to the interaction of its major compound, piperine (1-piperoyl-piperidine) with the human TRPV-1 or vanilloid receptor. We now identify the hitherto concealed enzymatic formation of piperine from piperoyl coenzyme A and piperidine based on a differential RNA-Seq approach from developing black pepper fruits. This enzyme is described as piperine synthase (piperoyl-CoA:piperidine piperoyl transferase) and is a member of the BAHD-type of acyltransferases encoded by a gene that is preferentially expressed in immature fruits. A second BAHD-type enzyme, also highly expressed in immature black pepper fruits, has a rather promiscuous substrate specificity, combining diverse CoA-esters with aliphatic and aromatic amines with similar efficiencies, and was termed piperamide synthase. Recombinant piperine and piperamide synthases are members of a small gene family in black pepper. They can be used to facilitate the microbial production of a broad range of medicinally relevant aliphatic and aromatic piperamides based on a wide array of CoA-donors and amine-derived acceptors, offering widespread applications.
Direct visualization of radiation-induced transformations at alkali halide–air interfaces
Authors: Riechers Shawn L. , Petrik Nikolay G. , Loring John S. , Bowden Mark E. , Cliff John B. , Murphy Mark K. , Pearce Carolyn I. , Kimmel Greg A. , Rosso Kevin M. 
Published: April 2021

AbstractRadiation driven reactions at mineral/air interfaces are important to the chemistry of the atmosphere, but experimental constraints (e.g. simultaneous irradiation, in situ observation, and environmental control) leave process understanding incomplete. Using a custom atomic force microscope equipped with an integrated X-ray source, transformation of potassium bromide surfaces to potassium nitrate by air radiolysis species was followed directly in situ at the nanoscale. Radiolysis initiates dynamic step edge dissolution, surface composition evolution, and ultimately nucleation and heteroepitaxial growth of potassium nitrate crystallites mediated by surface diffusion at rates controlled by adsorbed water. In contrast to in situ electron microscopy and synchrotron-based imaging techniques where high radiation doses are intrinsic, our approach illustrates the value of decoupling irradiation and the basis of observation.
Metabolic engineering of Vibrio natriegens
Authors: Thoma Felix , Blombach Bastian 
Published: April 2021

Abstract
Vibrio natriegens is emerging as a promising host for biotechnology which is basically due to the remarkable intrinsic properties such as the exceptionally high growth and substrate consumption rates. The facultatively anaerobic marine bacterium possesses a versatile metabolism, is able to utilize a variety of substrates as carbon and energy sources and is easy to handle in the lab. These features initiated the rapid development of genetic tools and resulted in extensive engineering of production strains in the past years. Although recent examples illustrate the potential of V. natriegens for biotechnology, a comprehensive understanding of the metabolism and its regulation is still lacking but essential to exploit the full potential of this bacterium. In this review, we summarize the current knowledge on the physiological traits and the genomic organization, provide an overview of the available genetic engineering tools and recent advances in metabolic engineering of V. natriegens. Finally, we discuss the obstacles which have to be overcome in order to establish V. natriegens as industrial production host.
A Pilot Single-Case Experimental Design Study of Rapid Syllable Transition Treatment for Italian Children With Childhood Apraxia of Speech
Authors: Scarcella Ilaria , Michelazzo Letizia , McCabe Patricia 
Published: April 2021

Background
The Rapid Syllable Transition (ReST) treatment is an effective intervention designed to address the planning and programming disorder found in childhood apraxia of speech. To date, no study has considered its use with children who speak languages other than English.

Aim
This pilot study aimed to examine the use of ReST treatment with Italian children. We hypothesized that the ReST approach would improve the overall accuracy of the targeted behaviors of lexical stress, smoothness, and phonemic accuracy, whereas phonemic and phonetic accuracy of untreated items would remain stable.

Method
Two monolingual Italian-speaking children with childhood apraxia of speech received therapy in 12 one-hour sessions, 2–3 times per week, using a single-case experimental design. The treatment procedures used in English were replicated in Italian with one change: In English, two stress patterns are treated. Italian commonly uses three stress patterns; therefore, the stimuli were modified accordingly. Accuracy of articulation, lexical stress, and smoothness were assessed at pretreatment and 1 day, 1 week, and 4 months posttreatment in treated and untreated pseudowords and in real words and sentences.

Results
Both children improved on treated pseudowords and real words with moderate effect sizes, but only one child generalized to untreated pseudowords. Maintenance of treatment results was observed in both participants. Articulation of control phonemes did not change.

Conclusions
ReST treatment in Italian is feasible, and a treatment effect was found, showing that its use may be valid in languages other than English. Further research is required.

Supplemental Material

https://doi.org/10.23641/asha.14348060
The Effect of Narrative Language Intervention on the Language Skills of Children With Hearing Loss
Authors: Raymond Stephanie M. , Spencer Trina D. 
Published: April 2021

Purpose
Narrative intervention has not been extensively investigated with children with hearing loss, but it has been shown to improve a broad range of language skills of children with a variety of disabilities and language needs. Therefore, the purpose of this study was to investigate the effect of narrative language intervention on the narrative retelling skills and vocabulary use of children with hearing loss.

Method
A multiple baseline design (for retelling) and a repeated acquisition design (for vocabulary) were used to fulfill the purpose of the study. Participants included two children ages 5 and 9 years diagnosed with bilateral sensorineural hearing loss, fitted to an amplification device. Each child received one 70-min session of individual narrative language intervention per week for at least 6 weeks that focused on teaching less common vocabulary words in addition to story grammar and complex sentences.

Results
Both participants demonstrated weekly increases in narrative retell scores and repeated pretest to posttest gains in the use of targeted vocabulary. Evidenced through visual analysis, both participants showed some growth in retell once intervention was introduced, with at least a modest upward trend each week. Moreover, vocabulary use scores, collected directly after intervention, showed both participants improved vocabulary use in familiar and untrained contexts.

Conclusions
Results suggest narrative language intervention improved the narrative retell ability and vocabulary use of children with hearing loss. Narrative intervention is a promising approach for promoting the language skills of children with hearing loss, but this finding requires replication.
Multiscale fully convolutional network‐based approach for multilingual character segmentation
Authors: Yu Chao , Liu Jin , Li Yunhui 
Published: April 2021

AbstractCharacter segmentation is a challenging task for optical character recognition systems. Traditional methods usually utilize rule‐based algorithms but most of them are not applicable in modern intelligent recognition applications that require high accuracy. It is especially the case for text containing Eastern Asian language characters with complex pictograph structures, such as Chinese. To alleviate this problem, this study proposes an encoder–decoder structure‐based multiscale fully convolutional network (MSFCN) model for optical character segmentation. Comparing with other methods, MSFCN can not only effectively extract semantic details from images but also exploit boundary information of intervals between characters, thereby distinguishing characters from a background in pixel level. Extensive experiments have been conducted on two benchmark data sets of ICDAR2013 and MLCS. Obtained results prove that MSFCN achieves state‐of‐the‐art segmentation performance and indicated its practical application value.
Tracking‐DOSeqSLAM: A dynamic sequence‐based visual place recognition paradigm
Authors: Tsintotas Konstantinos A. , Bampis Loukas , Gasteratos Antonios ,   FIET
Published: April 2021

AbstractSimultaneous localization and mapping (SLAM) refers to a process that permits a mobile robot to build up a map of the environment and, at the same time, to use it to compute its location. One of its most important components is its ability to associate the most recently perceived visual measurement to the one derived from previsited locations, a technique widely known as loop closure detection. In this article, we evolve our previous approach, dubbed as ‘DOSeqSLAM’ by presenting a low complexity loop closure detection pipeline wherein the traversed trajectory (map) is represented by sequence‐based locations (submaps). Each of these groups of images, referred to as place, is generated online through a point tracking repeatability check employed on the perceived visual sensory information. When querying the database, the proper candidate place is selected and, through an image‐to‐image search, the appropriate location is chosen. The method is subjected to an extensive evaluation on seven publicly available datasets, revealing a substantial improvement in computational complexity and performance over its predecessors, while performing favourably against other state‐of‐the art solutions. The system’s effectiveness is owed to the reduced number of places, which, compared to the original approach, is at least one order of magnitude less.
Optimal device capacity planning and strategy determination in an integrated energy system to promoting IES integration considering reliability value
Authors: Gao Xueqian , Liu Wenxia 
Published: April 2021

AbstractThis paper proposes a novel framework for optimal planning of integrated energy system (IES) that takes into account its reliability value, which aims at promoting the power grid and IES to share the responsibility of the user's energy‐supply reliability by market mechanism, thus promoting IES integration. First, an IES grid‐connected electricity purchase price estimation model considering reliability is established, based on price theory and reliability theory, to quantify the reliability incremental value of IES. Then, a two‐layer collaborative planning model, containing optimal equipment capacity configuration and strategy determination in the upper layer and optimal operation simulation and IES incremental reliability value in the lower layer, is established to coordinate the reliability guarantee capability of distribution network and IES while maximize the profit of IES. Among them, a practical approach of IES probabilistic reliability calculation is studied, considering double uncertainty and time‐series matching of generation and demand, aiming at reducing the complexity of electricity price calculation embedded in planning model. Next, the intelligent krill herd algorithm and MILP are implemented to solve the model. Finally, a case is studied to verify the effectiveness of the model, and analyse the impact of load growth rate and user reliability requirements on the planning results.