bluuebunny/crossref_metadata_2025_split huggingface dataset:
Genetic Interactions Between HOP1, RED1 and MEK1 Suggest That MEK1 Regulates Assembly of Axial Element Components During Meiosis in the Yeast Saccharomyces cerevisiae
Authors: Hollingsworth Nancy M , Ponte Lisa 
Published: January 2021

Abstract
During meiosis, axial elements are generated by the condensation of sister chromatids along a protein core as precursors to the formation of the synaptonemal complex (X). Functional axial elements are essential for wild-type levels of recombination and proper reductional segregation at meiosis I. Genetic and cytological data suggest that three meiosis-specific genes, HOP1, RED1 and MEK1, are involved in axial element formation in the yeast Saccharomyces cerevisiae. HOP1 and RED1 encode structural components of axial elements while MEK1 encodes a putative protein kinase. Using a partially functional allele of MEK1, new genetic interactions have been found between HOP1, RED1 and MEK1. Overexpression of HOP1 partially suppresses the spore inviability and recombination defects of mekl-974; in contrast, overexpression of RED1 exacerbates the mek1-974 spore inviability. Co-overexpression of HOP1 and RED1 in mek1-974 diploids alleviates the negative effect of overexpressing RED1 alone. Red1p/Red1p as well as Hop1p/Red1p interactions have been reconstituted in two hybrid experiments. Our results suggest a model whereby Mekl kinase activity controls axial element assembly by regulating the affinity with which Hoplp and Redlp interact with each other.
RDH54, a RAD54 Homologue in Saccharomyces cerevisiae, Is Required for Mitotic Diploid-Specific Recombination and Repair and for Meiosis
Authors: Klein Hannah L 
Published: January 2021

Abstract
Most mitotic recombination and repair genes of Saccharomyces cerevisiae show no specificity of action for the genome ploidy. We describe here a novel repair and recombination gene that is specific for recombination and repair between homologous chromosomes. The RDH54 gene is homologous to the RAD54 gene, but rdh54 mutants do not show sensitivity to methyl methanesulfonate at concentrations that sensitize a rad54 mutant. However, the rdh54 null mutation enhances the methyl methanesulfonate sensitivity of a rad54 mutant and single rdh54 mutants are sensitive to prolonged exposure at high concentrations of methyl methanesulfonate. The RDH54 gene is required for recombination, but only in a diploid. We present evidence showing that the RDH54 gene is required for interhomologue gene conversion but not intrachromosomal gene conversion. The rdh54 mutation confers diploid-specific lethalities and reduced growth in various mutant backgrounds. These phenotypes are due to attempted recombination. The RDH54 gene is also required for meiosis as homozygous mutant diploids show very poor sporulation and reduced spore viability. The role of the RDH54 gene in mitotic repair and in meiosis and the pathway in which it acts are discussed.
Control of Amino Acid Permease Sorting in the Late Secretory Pathway of Saccharomyces cerevisiae by SEC13, LST4, LST7 and LST78
Authors: Roberg Kevin J , Bickel Stephen , Rowley Neil , Kaiser Chris A 
Published: January 2021

Abstract
The SEC13 gene was originally identified by temperature-sensitive mutations that block all protein transport from the ER to the Golgi. We have found that at a permissive temperature for growth, the sec13-1 mutation selectively blocks transport of the nitrogen-regulated amino acid permease, Gaplp, from the Golgi to the plasma membrane, but does not affect the activity of constitutive permeases such as Hip1p, Can1p, or Lyp1p. Different alleles of SEC13 exhibit different relative effects on protein transport from the ER to the Golgi, or on Gap1p activity, indicating distinct requirements for SEC13 function at two different steps in the secretory pathway. Three new genes, LST4, LST7, and LSTB, were identified that are also required for amino acid permease transport from the Golgi to the cell surface. Mutations in LST4 and LST7 reduce the activity of the nitrogen-regulated permeases Gap1p and Put4p, whereas mutations in LST8 impair the activities of a broader set of amino acid permeases. The LST8 gene encodes a protein composed of WD-repeats and has a close human homologue. The LST7 gene encodes a novel protein. Together, these data indicate that SEC13, LST4, LST7, and LST8 function in the regulated delivery of Gap1p to the cell surface, perhaps as components of a post-Golgi secretory-vesicle coat.
Mutations Synthetically Lethal With tpm1Δ Lie in Genes Involved in Morphogenesis
Authors: Wang Tongtong , Bretscher Anthony 
Published: January 2021

Abstract
Yeast contains two genes, TPM1 and TPM2, encoding tropomyosins, either of which can provide an essential function in the yeast cytoskeleton. To elucidate more clearly the function of the major tropomye sin, encoded by TPM1, we have isolated mutations that confer synthetic lethality with the null mutant of TPM1. Here we describe a phenotypic and genetic analysis of mutations in TSL1/BEM2, TSL2, TSL3, TSL5, and TSL6 (tropomyosin synthetic lethal). All the mutants exhibit clear morphological and some actin cytoskeletal defects, but are not noticeably defective in secretion, endocytosis, or organelle segregation. The lethality conferred by tsl tpm1Δ mutations could be specifically suppressed by either TPM1 or an additional copy of TPM2. This implies that the essential function compromised in the tsl tpm1Δ constructs is the same essential function for which Tpm1p or Tpm2p is necessary. Synthetic interactions and unlinked noncomplementation were observed between the tsl mutants, suggesting that they participate in related functions involving morphogenesis. In support of this, tsl6-1 was identified as an allele of the nonessential gene SLT2 or MPK1 whose product is a MAP kinase regulating cell wall synthesis. These results indicate that this synthetic lethality approach provides a sensitive screen for the isolation of mutations affecting morphogenesis, many of which are likely to be in nonessential genes, like BEM2 and SLT2.
Analysis of Mutationally Altered Forms of the Cct6 Subunit of the Chaperonin From Saccharomyces cerevisiae
Authors: Lin Paul , Cardillo Thomas S , Richard Lynn M , Segel George B , Sherman Fred 
Published: January 2021

Abstract
The Cct double-ring chaperonin complex of Saccharomyces cerevisiae is comprised of eight essential subunits, Cct1p-Cct8p, and assists the folding of substrates such as actins and tubulins. Single and multiple amino acid replacements of Cct6p were constructed by oligonucleotide-directed mutagenesis, including changes of charged to alanine residues and uncharged to charged residues. The replacements were targeted, in part, to residues corresponding to functionally critical regions identified in the published crystal structure of the Escherichia coli chaperonin, GroEL. Here, we report the critical hydrophobic residues and clusters of hydrophilic residues in regions corresponding to those from the apical domain of GroEL implicated in peptide binding and peptide release, and certain residues in the putative equatorial domain implicated in subunit-to-subunit interaction. In contrast to their homologous counterparts in Cct2p and Cct1p, the highly conserved putative ATP binding motifs of Cct6p were relatively amenable to mutations. Our data suggest that the entire Cct6p molecule might be essential for assembly of Cct complex and might participate in binding substrates. However, there appeared to exist a functional hierarchy in ATP binding/hydrolysis among Cct subunits, as suggested by the high tolerance of Cct6p to mutations within the putative ATP binding pocket.
Calmodulin Point Mutations Affect Drosophila Development and Behavior
Authors: Nelson Heidi B , Heiman Robert G , Bolduc Clare , Kovalick Gae E , Whitley Penn , Stern Michael , Beckingham Kathy 
Published: January 2021

Abstract
Calmodulin (CAM) is recognized as a major intermediary in intracellular calcium signaling, but as yet little is known of its role in developmental and behavioral processes. We have generated and studied mutations to the endogenous Cam gene of Drosophila melanogaster that change single amino acids within the protein coding region. One of these mutations produces a striking pupal lethal phenotype involving failure of head eversion. Various mutant combinations produce specific patterns of ectopic wing vein formation or melanotic scabs on the cuticle. Anaphase chromosome bridging is also seen as a maternal effect during the early embryonic nuclear divisions. In addition, specific behavioral defects such as poor climbing and flightlessness are detected among these mutants. Comparisons with other Drosophila mutant phenotypes suggests potential CAM targets that may mediate these developmental and behavioral effects, and analysis of the CAM crystal structure suggests the structural consequences of the individual mutations.
The TRANSFORMER Genes of the Fern Ceratopteris Simultaneously Promote Meristem and Archegonia Development and Repress Antheridia Development in the Developing Gametophyte
Authors: Banks Jo Ann 
Published: January 2021

Abstract
The sex of the haploid gametophyte of the fern Ceratopteris is determined by the presence or absence of the pheromone antheridiogen, which, when present, promotes male development and represses female development of the gametophyte. Several genes involved in sex determination in Ceratopteris have been identified by mutation. In this study, the epistatic interactions among new and previously described sex-determining mutants have been characterized. These results show that sex expression is regulated by two sets of genes defined by the FEM1 and TRA loci. Each promotes the expression of either male or female traits and simultaneously represses the expression of the other. A model describing how antheridiogen regulates the expression of these genes and the sex of the gametophyte is described. The observation that some gametophytic sexdetermining mutants have phenotypic effects on the spore phyte plant indicates that sex determination in the Ceratopteris gametophyte is regulated by a mechanism that also regulates sporophyte development.
B Chromosome Behavior in Maize Pollen as Determined by a Molecular Probe
Authors: Rusche M Losoff , Mogensen H Lloyd , Shi Liang , Keim Paul , Rougier Mireille , Chaboud Annie , Dumas Christian 
Published: January 2021

Abstract
The B chromosomes of maize typically undergo nondisjunction during the second microspore division (generative cell division). When the microspore nucleus contains only one B chromosome, two kinds of sperm result, one with two B chromosomes and one with no B chromosomes. The sperm with the B chromosomes preferentially fertilizes the egg cell. Previous studies of these phenomena have been limited to genetic analysis and chromosome spreads. In this study we show that a B chromosome-specific probe can be used with fluorescence in situ hybridization (FISH) analysis to detect the presence, location, and frequency of B chromosomes in intact interphase nuclei within mature pollen of maize. Using genetic line TB-10L18, our results indicate that nondisjunction of the B centromere occurs at an average frequency of 56.6%, based on four plants and 1306 pollen grains analyzed. This is consistent with the results of genetic studies using the same B-A translocation. In addition, our results suggest that B chromosome nondisjunction can occur during the first microspore division. Spatial distribution of the B chromosomespecific probe appears to be largely confined to one tip of the sperm nucleus, and a DNA fragment found outside the pollen nuclei often hybridizes to the B chromosomespecific probe.
The effect of education based on PEN-3 cultural model on students' menstrual health behaviors: a mixed method study
Authors: Mohammadinia Neda , Rezaei Mohammad Ali , Morowatisharifabad Mohammad Ali , Heydarikhayat Nastaran 
Published: January 2021

AbstractMenstruation is a natural process in girls, but sometimes it is accompanied by beliefs and behaviors with cultural roots that result in poor health consequences; this study aimed to consider perceptions of individual, families and community to current cultural beliefs, and to determine the effect of education based on the PEN-3 cultural model on students' menstrual health behaviors in Iran. Study had a mixed method design. In the qualitative phase, data were collected from students, their mothers and teachers through focused group discussion and in-depth interviews. In the quantitative phase, training was done in four 2-h sessions for intervention group. The data were collected immediately and 2 months after the training by a questionnaire and they were analyzed by Friedman and Wilcoxon non-parametric tests. Training was effective on all of the model structures and significantly increased mean score of the health behavior after training and 2 months later by 8.74 and 13.86, respectively, in intervention group (P&lt;0.05). The perception and behavior of the others and access to sanitary services and products, especially cultural factors affect girls’' menstrual health behaviors, therefore, it is necessary to design the health plans regarding each of these factors, and the cultural context of each community.
Using Deep Learning to Improve Nonsystematic Viewing of Breast Cancer on MRI
Authors: Eskreis-Winkler Sarah , Onishi Natsuko , Pinker Katja , Reiner Jeffrey S , Kaplan Jennifer , Morris Elizabeth A , Sutton Elizabeth J 
Published: January 2021

Abstract

Objective
To investigate the feasibility of using deep learning to identify tumor-containing axial slices on breast MRI images.

Methods
This IRB–approved retrospective study included consecutive patients with operable invasive breast cancer undergoing pretreatment breast MRI between January 1, 2014, and December 31, 2017. Axial tumor-containing slices from the first postcontrast phase were extracted. Each axial image was subdivided into two subimages: one of the ipsilateral cancer-containing breast and one of the contralateral healthy breast. Cases were randomly divided into training, validation, and testing sets. A convolutional neural network was trained to classify subimages into “cancer” and “no cancer” categories. Accuracy, sensitivity, and specificity of the classification system were determined using pathology as the reference standard. A two-reader study was performed to measure the time savings of the deep learning algorithm using descriptive statistics.

Results
Two hundred and seventy-three patients with unilateral breast cancer met study criteria. On the held-out test set, accuracy of the deep learning system for tumor detection was 92.8% (648/706; 95% confidence interval: 89.7%–93.8%). Sensitivity and specificity were 89.5% and 94.3%, respectively. Readers spent 3 to 45 seconds to scroll to the tumor-containing slices without use of the deep learning algorithm.

Conclusion
In breast MR exams containing breast cancer, deep learning can be used to identify the tumor-containing slices. This technology may be integrated into the picture archiving and communication system to bypass scrolling when viewing stacked images, which can be helpful during nonsystematic image viewing, such as during interdisciplinary tumor board meetings.
Space as a Resource in the Politics of Consumer Identity
Authors: Maciel Andre F , Wallendorf Melanie 
Published: January 2021

AbstractConsumers can pursue a wide range of market-mediated identities in contemporary culture. However, some consumer identities are more valued than others, creating a form of cultural inequality. The present research considers consumers’ deliberate efforts to assert greater cultural value for their identities, a phenomenon termed a “politicized consumer identity project.” Specifically, this research focuses on consumers’ intentional use of space, a resource that is ubiquitous in social life but has, nonetheless, received limited theoretical attention regarding this type of identity project. This ethnography uses mixed methods to study a sample of women embedded in the new cult of domesticity, an ethos that induces participants to use various spaces as a way of claiming greater value for feminine consumer identities that are often demeaned by others. The results reveal a system of spatial practices that consumers employ to contest this cultural devaluation across a broad range of sites in their lives, from their homes to commercial and public venues. We conceptualize these practices as spatial affirmation, repurposing, and incursion, showing their ideological and material interdependencies. This research advances understanding of the ties among consumer identities, space, and cultural politics.
Idiopathic myointimal hyperplasia of the mesenteric veins
Authors: Al Ansari Ali , Ahmed Shehab , Mansour Eyad , Abass Maher A 
Published: January 2021

Abstract
Idiopathic myointimal hyperplasia of the mesenteric veins (IMHMV) is caused by proliferation of smooth muscle cells in the wall of small mesenteric veins and venules with accumulation of a proteoglycan matrix leading to a non-thrombotic, non-inflammatory venous occlusion resulting in venous ischemia. IMHMV is a rare and poorly understood disease, with &lt;20 case reports in the literature. The purpose of this report is to describe the case of a 63-year-old man who presented with this condition that resulted in colonic ischemia necessitating surgical resection. The cause of IMHMV in this patient was attributed to a Chinese herbal supplement used for degenerative osteoarthritis of the knees. A brief review of the literature is provided along with the case report.
From hydroxychloroquine to ivermectin: what are the anti-viral properties of anti-parasitic drugs to combat SARS-CoV-2?
Authors: Rakedzon S , Neuberger A , Domb A J , Petersiel N , Schwartz E 
Published: January 2021

Abstract

Background
Nearly a year into the COVID-19 pandemic, we still lack effective anti-SARS-CoV-2 drugs with substantial impact on mortality rates except for dexamethasone. As the search for effective antiviral agents continues, we aimed to review data on the potential of repurposing antiparasitic drugs against viruses in general, with an emphasis on coronaviruses.

Methods
We performed a review by screening in vitro and in vivo studies that assessed the antiviral activity of several antiparasitic agents: chloroquine, hydroxychloroquine (HCQ), mefloquine, artemisinins, ivermectin, nitazoxanide (NTZ), niclosamide, atovaquone and albendazole.

Results
For HCQ and chloroquine we found ample in vitro evidence of antiviral activity. Cohort studies that assessed the use of HCQ for COVID-19 reported conflicting results, but randomized controlled trials (RCTs) demonstrated no effect on mortality rates and no substantial clinical benefits of HCQ used either for prevention or treatment of COVID-19. We found two clinical studies of artemisinins and two studies of NTZ for treatment of viruses other than COVID-19, all of which showed mixed results. Ivermectin was evaluated in one RCT and few observational studies, demonstrating conflicting results. As the level of evidence of these data is low, the efficacy of ivermectin against COVID-19 remains to be proven. For chloroquine, HCQ, mefloquine, artemisinins, ivermectin, NTZ and niclosamide, we found in vitro studies showing some effects against a wide array of viruses. We found no relevant studies for atovaquone and albendazole.

Conclusions
As the search for an effective drug active against SARS-CoV-2 continues, we argue that pre-clinical research of possible antiviral effects of compounds that could have antiviral activity should be conducted. Clinical studies should be conducted when sufficient in vitro evidence exists, and drugs should be introduced into widespread clinical use only after being rigorously tested in RCTs. Such a search may prove beneficial in this pandemic or in outbreaks yet to come.
Abstract argumentation and the rational man
Authors: Kampik Timotheus , Nieves Juan Carlos 
Published: January 2021

Abstract
Abstract argumentation has emerged as a method for non-monotonic reasoning that has gained popularity in the symbolic artificial intelligence community. In the literature, the different approaches to abstract argumentation that were refined over the years are typically evaluated from a formal logics perspective; an analysis that is based on models of economically rational decision-making does not exist. In this paper, we work towards addressing this issue by analysing abstract argumentation from the perspective of the rational man paradigm in microeconomic theory. To assess under which conditions abstract argumentation-based decision-making can be considered economically rational, we derive reference independence as a non-monotonic inference property from a formal model of economic rationality and create a new argumentation principle that ensures compliance with this property. We then compare the reference independence principle with other reasoning principles, in particular with cautious monotony and rational monotony. We show that the argumentation semantics as proposed in Dung’s seminal paper, as well as other semantics we evaluate, with the exception of naive semantics and the SCC-recursive CF2 semantics, violate the reference independence principle. Consequently, we investigate how structural properties of argumentation frameworks impact the reference independence principle and identify cyclic expansions (both even and odd cycles) as the root of the problem. Finally, we put reference independence into the context of preference-based argumentation and show that for this argumentation variant, which explicitly models preferences, reference independence cannot be ensured in a straight-forward manner.
A NHS Foundation Trust v MC [2020] EWCOP 33: Revisiting Best Interests and ‘Altruistic’ Incapacitous Stem Cell Donation
Authors: Venter Bonnie 
Published: January 2021

Abstract
In A NHS Foundation Trust v MC, the Court of Protection revisits the question of whether adults should be allowed to act as bone marrow or peripheral blood stem cell donors if they lack decision-making capacity. This case note explores the positive and problematic implications of the case based on points that were raised in the judicial reasoning that specifically relate to i) practical implications concerning the key players in this environment, ii) the risk analysis within the best interest determination, iii) altruism and iv) the wider context as it relates to minor donors who lack capacity.