bluuebunny/crossref_metadata_2025_split huggingface dataset:
Plant ontogeny as a driver of elevational patterns of entomopathogenic nematode activity in Mediterranean oaks
Authors: Blanco-Pérez Rubén , Galmán Andrea , Pou Alicia , Moreira Xoaquín 
Published: February 2025

Abstract

Background and aims
A long-standing ecological assumption posits that insect herbivory increases in warmer, more stable climates at lower elevations. However, this paradigm has been called into question in recent decades. Some studies suggest that differences in tri-trophic interactions, particularly the diversity, abundance, and activity of herbivore natural enemies, may explain inconsistent patterns in herbivory. Additionally, plant ontogeny significantly influences herbivore susceptibility, with adult plants being more apparent and thus more susceptible to herbivore attacks than saplings. These ontogenetic differences in herbivory might, in turn, determine changes in herbivore predation across elevations. Unfortunately, most research addressing these ecological assumptions has focused on aboveground tri-trophic interactions.

Methods
Here, we investigated elevational differences in the activity of entomopathogenic nematodes (EPNs), known killers of soil-dwelling insects, and compared these patterns between young and adult oak (Quercus, Fagaceae) trees. We collected soil samples from rhizospheres of adult trees and saplings throughout the optimal elevational range (low, mid, and high) of 10 Mediterranean oak species in the Iberian Peninsula, estimating EPN activity through insect baiting with wax moth larvae.

Results
Our results showed higher larval mortality and EPN activity at lower elevations, with this effect being influenced by plant ontogeny; therefore, elevation-related variations were observed only in rhizospheres of mature trees. Additionally, we found that soil characteristics did not significantly affect these outcomes.

Conclusions
Our study provides evidence that plant ontogeny influences belowground tri-trophic interactions along elevational gradients in oak species, emphasizing the minimal impact of abiotic soil factors on these processes.
Tracer distribution in legume roots and soluble rhizodeposits over a few weeks after a triple isotope (13C, 15N, 33P) labeling
Authors: Stevenel Pierre , Abiven Samuel , Frossard Emmanuel , Rao Idupulapati M. , Tamburini Federica , Oberson Astrid 
Published: February 2025

Abstract

Background and aims
Belowground carbon (C), nitrogen (N) and phosphorus (P) inputs by plants via roots and rhizodeposition are key drivers of these elements cycling in soils. Tracing and quantification of rhizodeposition using isotope enrichment techniques is based on assumptions that have not simultaneously been tested for C, N and P. Our objectives were: (i) to compare the elemental and isotopic composition (IC) of roots and soluble rhizodeposits for C, N and P; and (ii) to compare the IC of root segments of different ages to assess the homogeneity of root system labeling.

Methods
The legume Canavalia brasiliensis was grown in sand and labeled with 13C, 15N and 33P by using a novel tri-isotope method in two different experiments lasting two (percolate collection) and three weeks (root observation) after labeling.

Results
Soluble rhizodeposits were less isotope enriched than roots at each time point, and each element showed a different course over time. The 13C:15N and 13C:33P ratios of rhizodeposits were higher shortly after labeling than at later samplings, highlighting faster transfer of 13C than 15N and 33P into rhizodeposits. Phosphorus fertilizer increased the difference between IC of P in roots and rhizodeposits. Youngest root segments were more isotopically enriched than older segments, again with element specific time course, showing that root segments of different ages differ in IC.

Conclusion
Assumptions underlying the quantification of rhizodeposition are not met. Temporal and spatial differences in IC of roots and soluble rhizodeposits are element specific, which needs to be considered in stoichiometric studies.
Sprouting Control Treatment with LED Lights Does Not Affect the Biochemical Aspects of ‘Asterix’ Potatoes
Authors: Pedrosa Vanessa Maria Dantas , Izidoro Maiqui , da Silva Maryelle Barros , da Silva Francisco Ronaldo Cardoso , Cecilio Filho Arthur Bernardes , Carvalho Rogério Falleiros , Bacha Allan Lopes , Teixeira Gustavo Henrique de Almeida 
Published: February 2025

Abstract
Recently, it was observed that red, blue and far-red lights and different intensities (0.1 and 1.0 μmol m−2 s−1) of light-emitting diode (LED) reduced potato sprout growth but did not effectively inhibit sprouting in physiologically aged tubers. Therefore, this study aimed to identify the physiological and biochemical changes and sprout development in potatoes treated with LED lights or with 3-chlorophenyl carbamate (CIPC). To achieve this, ‘Asterix’ potatoes were harvested, cured at 15 °C (90–93% RH) for 15 days and treated with red (5 µmol m−2 s−1), far-red (5 µmol m−2 s−1) and blue (1 µmol m−2 s−1) LED lights, in addition to the negative control (dark) and positive control (0.022 g L−1 of CIPC) treatments. Potatoes from the light treatments were exposed to LED lights 24 h a day throughout the storage period, under optimal conditions for sprout development (16.7 ± 1 °C and 91.5 ± 1% RH). Tubers treated with blue and far-red LED light exhibited longer dormancy, fewer sprouts and smaller sprouts over the storage period. The carbohydrate content (starch and reducing sugars) was not affected by light treatments; however, total soluble sugar content was lower in tubers kept in the dark and higher in those treated with far-red LED light, which was not related to sprouting control. Therefore, it is suggested that other signalling mechanisms may be involved in sprout control using LED lights.
Post-fire Quercus mycorrhizal associations are dominated by Russulaceae, Thelephoraceae, and Laccaria in the southern Appalachian Mountains
Authors: Hughes Karen W. , Franklin Jennifer A. , Schweitzer Jennifer , Kivlin Stephanie N. , Case Alexis , Aldrovandi Matthew , Matheny P. Brandon , Miller Andrew N. 
Published: February 2025

Abstract
Following disturbances such as wildfires, oak seedlings must form a symbiotic association with mycorrhizal fungi to survive. Wildfires, however, reduce available mycorrhizal fungal propagules in the soil. Ectomycorrhizal (ECM) fungi on oak seedlings sampled in severely burned (7 sites), moderately burned (7 sites), and unburned areas (8 sites) in the Great Smoky Mountains National Park were evaluated 21 months after the 2016 Chimney Tops 2 Wildfire by Sanger sequencing of the nuclear ribosomal DNA internal transcribed spacer region (nrITS; fungal barcode). Sequences were aligned and grouped into Operational Taxonomic Units (OTUs) based on well-supported phylogenetic clades and 98–100% nrITS sequence homology with sequences in GenBank. One hundred seventy-nine root-associated fungi comprising 124 OTUs were recovered after removing duplicates (the same fungus on two or more roots of the same plant). The ECM genus Russula was the most diverse genus (25 OTUs), followed by the Thelephora/Tomentella clade (18 OTUs), Lactifluus (8 OTUs), Lactarius (4 OTUs), and Laccaria aff. laccata (2 OTUs). Russula OTUs were identified more frequently on oak roots from burned areas and in burned soils, suggesting that some Russula taxa may have a selective advantage in burned areas. High alpha diversity occurred within each of the burn categories, but little overlap of taxa occurred between burn categories (high beta diversity). Approximately half of the recovered OTUs (100/179 total root-associated fungi = 55.9%) were found on a single plant. Oak seedlings growing in moderately and severely burned areas 21 months after a fire were capable of forming root associations with available fungi. In contrast to the expectation that root-associated fungal diversity would be reduced after a wildfire, diversity 1 year after the Chimney Tops 2 Fire was high with ectomycorrhizal Laccaria, Russulaceae, and Thelephoraceae dominating. This study suggests that the availability of ECM fungi post-fire is not a barrier to oak re-establishment.
Angiographic Anatomy of the Left Coronary Veins: Beyond Conventional Cardiac Resynchronization Therapy
Authors: Zehan Iulia-Georgiana , Eötvös Csilla-Andrea , Moldovan Mădălina Patricia , Andrei Mihai-Gabriel , Șchiop-Țentea Călina-Patricia , Lazar Roxana Daiana , Sârb Adriana Daniela , Coșeriu Giorgia , Avram Teodora , Șerban Adela , Gușetu Gabriel , Mocan-Hognogi Larisa Diana , Chiorescu Roxana , Pop Sorin , Heist Edwin Kevin , Blendea Dan 
Published: February 2025

Abstract

Purpose of Review
This review aims to synthesize current knowledge on the angiographic anatomy of the coronary sinus and its tributaries veins, with focus on venous branches other than classical ones used in cardiac resynchronization therapy. It also presents common anatomical aspects that could impact the clinical outcome.

Recent Findings
Recent advancements in the electrophysiology field, like epicardial arrhythmia mapping and ablation through coronary sinus or cardiac pacing from atypical veins requires a detailed angiographic assessment of cardiac veins. There is an increased interest for the veins coursing in the left ventricular summit (LVS) area and could potentially provide a pathway to reach the LVS arrhythmogenic foci. However, there is no consensus regarding the nomenclature and classification of these veins.

Summary
This review could offer a better understanding of the coronary sinus and its tributary veins distribution, dimensions and relationship with nearby structures that could help the development of new ablation and pacing tools and strategies, with higher success rates.
Kritik von Kindern und Jugendlichen im Kontext Schulsozialarbeit
Authors: Baier Florian 
Published: February 2025

Zusammenfassung
In diesem Beitrag wird auf der Grundlage empirischer Befunde aufgezeigt und diskutiert, wie im Kontext der Schulsozialarbeit mit kritischen Meinungen von Kindern und Jugendlichen in Bezug auf Schulsozialarbeit und Schule umgegangen wird. Es zeigt sich, dass kritischen Stimmen von Kindern und Jugendlichen mitunter wenig Gehör geschenkt wird, obwohl diese nicht nur für das Wohlergehen der Kinder und Jugendlichen, sondern auch für die Weiterentwicklung von Schulsozialarbeit und Schule sehr relevant sein können.
A population-based study of incidence trends of head and neck epithelial cancers in northeastern Spain, 1994–2018
Authors: Rubió-Casadevall Jordi , Trallero Jan , Calvo Carla , Puigdemont Montse , Carulla Marià , Sanvisens Arantza , Ameijide Alberto , Vidal Anna , Pla Clàudia , Marruecos Jordi , Marcos-Gragera Rafael , Galceran Jaume 
Published: February 2025

Abstract

Background
Head and neck cancer (HNC) is the seventh most common cancer worldwide. Incidence rates of these tumors vary between countries and change over time depending on the prevalence of risk factors such as tobacco and alcohol consumption, betel nut chewing habits or human papillomavirus infection. This makes it necessary to periodically study HNC incidence trends in each geographic area.

Methods
To determine trends in the incidence of HNC, all cancer cases diagnosed in Girona and Tarragona (northeastern Spain) between 1994 and 2018, except mesenchymal and hematological neoplasms, were included. Crude and standardized incidence rates and the annual percentage change (APC) were calculated.

Results
We identify 7,966 cases of HNC, 83.7% in men. A significant decrease in incidence, with an APC of  – 1.83, was observed in all HNC as a whole and in cancers of the lip (APC =  – 5.34), salivary glands (APC =  – 2.22), nasopharynx (APC =  – 2.01), hypopharynx (APC =  – 3.15), and larynx (APC =  – 1.97). In men, a significant decline in incidence was observed in overall HNC and in cancers of the lip, oral cavity, salivary glands, nasopharynx, hypopharynx, and larynx. In women, a significant increase was identified in overall HNC and in cancers of oral cavity, oropharynx, hypopharynx, and larynx.

Conclusion
A decline in the overall incidence of HNC has been observed in this area of southern Europe, mainly based on a decrease in men of cancers of the lip, oral cavity, salivary glands, nasopharynx, hypopharynx, and larynx.
Discriminant of tautological bundles on symmetric products of curves
Authors: Krug Andreas 
Published: February 2025

Abstract
We compute a formula for the discriminant of tautological bundles on symmetric powers of a complex smooth projective curve. It follows that the Bogomolov inequality does not give a new restriction to stability of these tautological bundles. It only rules out tautological bundles which are already known to have the structure sheaf as a destabilising subbundle.
Characterization of agr-like Loci in Lactiplantibacillus plantarum and L. paraplantarum and Their Role in Quorum Sensing and Virulence Inhibition of Staphylococcus aureus
Authors: Wang Weizhe , Kyrkou Ifigeneia , Bojer Martin S. , Kalloubi Dina , Kali Abdul Jabbar , Alena-Rodriguez Miguel , Leisner Jørgen J. , Fulaz Stephanie , Ingmer Hanne 
Published: February 2025

Abstract
The pathogenicity of Staphylococcus aureus is largely regulated by the agr quorum sensing (QS) system encoded by agrBDCA, which coordinates virulence factor production through secretion and sensing of auto-inducing peptides (AIPs). agr-like systems are also present in coagulase-negative staphylococci, and several of these encode AIPs that inhibit S. aureus QS. In lactic acid bacteria, a similar locus was previously identified in Lactiplantibacillus plantarum WCSF1 termed lamBDCA. Here, we characterized the lamBDCA locus in L. plantarum LMG 13556 and L. paraplantarum CIRM-BIA 1870, and explored the effects on S. aureus QS. Notably, we found that co-cultivation with L. paraplantarum significantly inhibits S. aureus QS and hemolysin production, while less so for L. plantarum. The inhibition by L. paraplantarum was lost upon disruption of its lamBDCA locus, suggesting that the L. paraplantarum AIP mediates cross-species interference with S. aureus agr activation. Transcriptomic analysis revealed that lamBDCA in L. paraplantarum controls the expression of genes belonging to various functional categories, including stress response and metabolism. The latter includes genes encoding riboflavin (B2 vitamin) biosynthesis, which enabled the growth of the L. paraplantarum lamB mutant in the presence of roseoflavin, a toxic riboflavin analogue. Collectively, our results show that L. paraplantarum CIRM-BIA 1870 interferes with S. aureus virulence gene expression through QS suppression, and they implicate QS in the probiotic properties of L. paraplantarum.
Genetically predicted vitamins supplementation and risk of skin cancers: a Mendelian randomization study
Authors: Qi Ping , Chen Liyun , Ma Aiwei , Zhang Yiwen , Lin Hai , Shi Wenqi , Huang Zijian , Tang Zixuan , Jiang Wenshi , Xu Mengjing , Zhang Wancong , Tang Shijie 
Published: February 2025

Abstract

Background
The causal relationship between vitamins supplementation and the risk of skin cancers remains unclear. We conducted a Mendelian randomization study to assess the associations between vitamins supplementation and skin cancers in the general population.

Objective
We aimed to investigate the causal relationship of vitamins (A-E) supplementation with skin cancers using and utilizing the linkage disequilibrium score regression (LDSC) Mendelian randomization (MR).

Materials and methods
We selected genome-wide significant single-nucleotide polymorphisms (SNPs) linked to vitamin supplements (A-E). Summary-level data for melanoma, basal cell carcinoma, and squamous cell carcinoma were obtained from large-scale genome-wide association studies. We applied the inverse-variance weighted (IVW) method within a random effects model, alongside weighted median, MR-Egger, simple median, sensitivity analyses, and MRlap methods to ensure robustness. A meta-analysis was conducted to assess the causal relationship between vitamins supplementation and melanoma. The STROBE-MR checklist was followed throughout.

Results
Folate supplementation was associated with a reduced melanoma risk (IVW OR = 0.88; 95% CI 0.80–0.96; P = 0.006). The MR analysis indicated a significant inverse causal relationship. Heterogeneity and sensitivity analyses confirmed minimal impact from individual SNPs. MRlap corrected for potential estimation bias due to sample overlap, which was not significant, reinforcing the IVW findings. The meta-analysis ensured robust and stable results. LDSC regression analysis suggests a weak causal relationship between folate supplementation and melanoma. Yet no association was found between genetically predicted vitamins A, B6, B12, C, D, E, and skin cancers.

Conclusion
Both observational meta-analysis and MR analysis based on genetic variation provide robust evidence indicat that folate supplementation decreases the risk of melanoma, suggesting that interventions targeting folate supplementation may contribute to the primary prevention of melanoma. Further studies are needed to explore the potential association between other vitamins supplementation and the risk of skin cancers.
Mapping and assessing impacts of land use land cover and climate conditions on groundwater quality using RS & GIS
Authors: Ghoto Sher Muhammad , Abbasi Habibullah , Memon Sheeraz Ahmed , Brohi Khan Muhammad , Chhachhar Rabia , Ghanghlo Asad Ali 
Published: February 2025

Abstract
Groundwater is an essential source for drinking purposes; hence, a qualitative analysis is necessary for groundwater resources. This study aims to assess the impacts of dynamic land use land cover (LULC) and climatic conditions on groundwater quality for drinking purposes. The investigative analysis of research used the water quality index (WQI) to analyze the groundwater quality and the source identified using the LULC map and climatic conditions. It extends an integrated and combined approach of different aspects. It provides a comprehensive understanding of how the various factors influence groundwater quality. The total area is classified as excellent, good, poor, very poor, and unfit for consumption based on the WQI. The results concluded that only 10.17% of the area has excellent drinking water quality, 19.97% has good water quality, 9.013% and 5.73% have poor and very poor water quality, respectively, and 55% of the water is unfit for consumption. The results indicated that the areas with agricultural expansion, urban development, and some natural conditions such as topographic features and high soil erodibility led to high total dissolved solids, electrical conductivity levels, and heavy metals. The main factors of LULC that lead to groundwater contamination include agricultural expansion and urban development. On the other hand, climatic conditions, such as variations in temperature and precipitation, also influenced groundwater quality. The research aids in examining different perspectives, which will lend a hand to water and land managers to make suitable decisions for sustainable development plans to conserve an economically important region.
Serotransferrin enhances transferrin receptor-mediated brain uptake of antibodies
Authors: Morrison Jamie I. , Metzendorf Nicole G. , Liu Jielu , Hultqvist Greta 
Published: February 2025

Abstract
The propensity of antibody-based therapies to systemically enter the brain interstitium and ameliorate pathology associated with numerous neurological maladies is precluded by the presence of the blood–brain barrier (BBB). Through distinct mechanisms, the BBB has evolved to regulate transport of essential ions, minerals, certain peptides and cells between the blood and the brain, but very restrictive otherwise. Hijacking receptor-mediated transport pathways of the BBB has proved fruitful in developing “Trojan Horse” therapeutic approaches to deliver antibody-based therapies to the brain milieu. The transferrin receptor (TfR)-mediated transcytosis pathway (RMT) is one such example where large recombinant molecules have been designed to bind to the TfR, which in turn activates the RMT pathway, resulting in delivery across the BBB into the brain milieu. Based on these findings, we here investigated whether the addition of serotransferrin could trigger the endogenous TfR-mediated RMT pathway and hence be used to enhance the uptake of TfR binding antibodies. By using an in vitro model of a mouse BBB we could test whether co-administration of mouse serotransferrin with mouse and human-based monoclonal antibodies enhanced brain uptake. In all cases tested, no matter if the monoclonal antibodies were designed to bind the TfR in a monovalent, partially monovalent/bivalent or entirely bivalent fashion, with high or low affinity or avidity, the addition of mouse serotransferrin significantly improved transport across the artificial BBB. This was also true for TfR binding antibodies that on their own passes the BBB poorly. These results were subsequently confirmed using a human in vitro BBB model, along with human serotransferrin and human TfR-binding antibody. To corroborate the in vitro results further, we conducted two pilot in vivo brain uptake study in wildtype mice, by intravenously co-administering a monoclonal TfR-binding antibody in the presence or absence of mouse serotransferrin as a proof-of-concept. In a similar outcome to the in vitro studies, we observed a significant almost two-fold increase in uptake of two different TfR binding antibodies in the brain when it was co-administered with mouse serotransferrin. These findings show for the first time that serotransferrin supplementation can significantly improve the ability of TfR-binding antibodies to traverse the BBB, which provides a realistic therapeutic opportunity for improving the delivery of therapeutic antibodies to the brain.

Graphical Abstract
When Clarity Clouds the View: Introducing a Decision Style Framework for Assessing Task-Related Effectiveness in Analysis and Intuition
Authors: Julmi Christian 
Published: February 2025

Abstract
Recent scholarly discussions suggest the potential superiority of intuition over analysis in tasks with high equivocality. However, it remains unclear when and whether intuition is preferable when both uncertainty and equivocality are considered. Addressing this gap, the article introduces a framework linking the effectiveness of individual decision styles with configurations of uncertainty and equivocality, adopting an information processing perspective. Within this framework, intuitiveness and adaptiveness are treated as independent dimensions of decision styles, associating intuitiveness with equivocality and adaptiveness with uncertainty in terms of effectiveness. To demonstrate the value of the framework, the article discusses implications for research.