bluuebunny/crossref_metadata_2025_split huggingface dataset:
A stress-reduced passaging technique improves the viability of human pluripotent cells
Authors: Takahashi Kazutoshi , Okubo Chikako , Nakamura Michiko , Iwasaki Mio , Kawahara Yuka , Tabata Tsuyoshi , Miyamoto Yousuke , Woltjen Knut , Yamanaka Shinya 
Published: October 2021

SummaryXeno-free culture systems have expanded the clinical and industrial application of human pluripotent stem cells (PSCs). However, yet some problems, such as the reproducibility among the experiments, remain. Here we describe an improved method for the subculture of human PSCs. The revised method significantly enhanced the viability of human PSCs by lowering DNA damage and apoptosis, resulting in more efficient and reproducible downstream applications such as gene editing, gene delivery, and directed differentiation. Furthermore, the method did not alter PSC characteristics after long-term culture and attenuated the growth advantage of abnormal subpopulations. This robust passaging method minimizes experimental error and reduces the rate of PSCs failing quality control of human PSC research and application.HighlightsThe revised passaging method significantly increases the viability of human PSCs.The method triggers less DNA damage and apoptosis signals compared to the conventional method.The stress-reduced method improves the results of downstream applications.The method does not alter PSC characters and attenuates the overgrowth of abnormal subpopulations.
Highly similar sequence and structure yet different biophysical behaviour: A computational study of two triosephosphate isomerases
Authors: Chávez-García Cecilia , Karttunen Mikko 
Published: October 2021

ABSTRACTHomodimeric triosephosphate isomerases (TIM) from Trypanosoma cruzi (TcTIM) and Trypanosoma brucei (TbTIM) have a markedly similar amino acid sequences and three-dimensional structures. However, several of their biophysical parameters, such as their susceptibility to sulfhydryl agents and their reactivation speed after being denatured, have significant differences. The causes of these differences were explored with microsecond-scale molecular dynamics (MD) simulations of three different TIM proteins: TcTIM, TbTIM and a chimeric protein, Mut1. We examined their electrostatic interactions and explored the impact of simulation length on them. The same salt bridge between catalytic residues Lys 14 and Glu 98 was observed in all three proteins but key differences were found in other interactions that the catalytic amino acids form. In particular, a cation-π interaction between catalytic amino acids Lys 14 and His 96, and both a salt bridge and a hydrogen bond between catalytic Glu168 and residue Arg100, were only observed in TcTIM. Furthermore, although TcTIM forms less hydrogen bonds than TbTIM and Mut1, its hydrogen bond network spans almost the entire protein, connecting the residues in both monomers. This work provides new insight on the mechanisms that give rise to the different behaviour of these proteins. The results also show the importance of long simulations.
A systematic review of construction safety research: quantitative and qualitative content analysis approach
Authors: Bhagwat Kishor , Delhi Venkata Santosh Kumar 
Published: October 2021

PurposeConstruction safety management (CSM) has been intensively researched in the last four decades but hitherto mostly aimed at understanding root causes of accidents, recommending preventive measures and evaluating their implications. However, a systematic effort to present a comprehensive picture of construction safety research is hardly witnessed. Therefore, the study aims to investigate construction safety research contributors, ontologies, themes, evolution, emerging trends and future directions using quantitative and qualitative content analysis.Design/methodology/approachA total of 877 journal articles were extracted using preferred reporting items for systematic reviews and meta-analyses (PRISMA) guidelines and Scopus literature database and were analyzed using VOSviewer and Nvivo tools to present a comprehensive picture of the CSM body of knowledge.FindingsThe study observed rapid growth in construction safety research with contributions from various countries, organizations and researchers. This study identified 3 research levels, 8 project phases, 10 project types, 6 research instruments and 19 research data sources along with their usage in the research domain. Further, the study identified 13 emerging research themes, 4 emerging research trends and an observed paradigm shift from reactive to proactive CSM approach.Research limitations/implicationsThe comprehensive study on the emerging themes and findings on proactive CSM has strategic implications to practice to incorporate safety. The identified future directions can assist researchers in bridging the existing gaps and strengthening emerging research trends.Originality/valueThe study presents a comprehensive picture of the CSM body of knowledge using the content analysis approach that was absent in past literature and opened future research avenues.
Internet public opinion dissemination mechanism of COVID-19: evidence from the Shuanghuanglian event
Authors: Zhang Xing , Zhou Yan , Zhou Fuli , Pratap Saurabh 
Published: October 2021

PurposeThe sudden outbreak of COVID-19 has become a major public health emergency of global concern. Studying the Internet public opinion dissemination mechanism of public health emergencies is of great significance for creating a legalized network environment, and it is also helpful for managers to make scientific decisions when encountering Internet public opinion crisis.Design/methodology/approachBased on the analysis of the process of spreading the Internet public opinion in major epidemics, a dynamic model of the Internet public opinion spread system was constructed to study the interactive relationship among the public opinion events, network media, netizens and government and the spread of epidemic public opinion. The Shuanghuanglian event in COVID-19 in China was taken as a typical example to make simulation analysis.FindingsResearch results show three points: (1) the government credibility plays a decisive role in the spread of Internet public opinion; (2) it is the best time to intervene when Internet public opinion occurred at first time; (3) the management and control of social media are the key to public opinion governance. Besides, specific countermeasures are proposed to assist control of Internet public opinion dissemination.Originality/valueThe epidemic Internet public opinion risk evolution system is a complex nonlinear social system. The system dynamics model is used to carry out research to facilitate the analysis of the Internet public opinion propagation mechanism and explore the interrelationship of various factors.
Determinants of mobile learning adoption: extending the unified theory of acceptance and use of technology (UTAUT)
Authors: Alowayr Ali 
Published: October 2021

PurposeAlthough several different learning technologies have been integrated into the face-to-face (F2F) learning approach, the effective implementation of mobile learning (m-learning) is still at an early stage. This may be due to the lack of understanding factors that affect learners' acceptance of m-learning.Design/methodology/approachConsidering the unified theory of acceptance and use of technology (UTAUT) as a theoretical background, this research investigates m-learning adoption in Saudi Arabia. The research framework extends the UTAUT by including intrinsic motivation, mobile learning self-efficacy and perceived satisfaction. A total of 200 higher education students voluntarily participated in the research. The application of the partial least square technique indicated that the proposed model can predict m-learning adoption adequately in Saudi Arabia (R2 = 0.73.8).FindingsThe research outcomes are significant for educational institutions and teachers to implement m-learning effectively. Many recommendations can be suggested to help enhance learners' willingness to adopt m-learning technology: Teachers need to design their courses in an interactive way and provide several different activities to ensure that learners obtain real benefits in their learning outcomes. When learners have positive perceptions about the use of this technology in the learning process, such perceptions should be supported by providing beneficial courses.Originality/valueThis research examines the effectiveness of the extended UTAUT to predict m-learning adoption in Saudi Arabia higher education. This could provide a significant contribution to the existing evidence about the validity of the model in different learning cultures and contexts.
Exploring teacher learning process in Chinese lesson study: a case of representing fractions on a number line
Authors: Huang Xingfeng , Huang Rongjin , Lai Mun Yee 
Published: October 2021

PurposeThis paper presented the learning process of a group of primary mathematics teachers who participated in two iterations of lesson design, enactment and reflection in a Chinese Lesson Study.Design/methodology/approachAn expansive learning theory was employed to examine the teachers’ learning process in lesson study (LS) on representing fractions on a number line. The evolution of a germ cell was utilized to feature the transformation of the object of activity from abstract to concrete through resolving contradictions among LS members. The videos of lesson planning, research lessons (RLs) and debriefing meetings were collected and analyzed to reveal the expansive learning process.FindingsThe analysis showed that the teachers expanded their learning through transforming the object from diffuse to concrete and expanded through consciously articulating the germ cell. The outcomes of object-oriented activity include improving the enacted lesson which promoted students’ conceptual understanding.Originality/valueThis study made a unique contribution to understanding the learning process of teachers in Chinese LS from the perspective of expansive learning.
Analysis of factors impacting survivability of sustainable supply chain during COVID-19 pandemic: an empirical study in the context of SMEs
Authors: Baral Manish Mohan , Singh Rajesh Kumar , Kazançoğlu Yiğit 
Published: October 2021

PurposeNowadays, many firms are finding ways to enhance the survivability of sustainable supply chains (SUSSCs). The present study aims to develop a model for the SUSSCs of small and medium enterprises (SMEs) during the COVID-19 pandemic.Design/methodology/approachWith the help of exhaustive literature review, constructs and items are identified to collect the responses from different SMEs. A total of 278 complete responses are received and 6 hypotheses are developed. Hypotheses testing have been done using structural equation modeling (SEM).FindingsMajor constructs identified for the study are supply chain (SC) performance measurement under uncertainty (SPMU), supply chain cooperation (SCCO), supply chain positioning (SCP), supply chain administration (SCA), supply chain feasibility (SCF) and the SUSSCs. From statistical analysis of the data collected, it can be concluded that the considered latent variables contribute significantly towardsthe model fit.Research limitations/implicationsThe present study contributes to the existing literature on disruptions and survivability. The study can be further carried out in context to different countries and sectors to generalize the findings.Practical implicationsThe research findings will be fruitful for SMEs and other organizations in developing strategies to improve survivability during uncertain business environments.Originality/valueThe study has developed a model that shows that the identified latent variables and their indicators contribute significantly toward the dependent variable, i.e. survivability. It contributes significantly in bridging the research gaps existing in context to the survivability of SMEs.
Family-owned enterprises and their role in entrepreneurial development: a bibliometric and content analysis of the literature
Authors: Kumar Rishi Kant , Dubey Amlendu Kumar 
Published: October 2021

Purpose
Family business has been widely discussed in the literature. Still, a holistic approach summarizing the family business concept in entrepreneurship is fragmented to date. This paper aims to explore the multimedia view of family business research in entrepreneurship and finds the key theme discussed by researchers.

Design/methodology/approach
In this paper, a systematic literature review on family business and its role in entrepreneurship have been conducted. The literature review consists of bibliometric and content analyzes. Bibliometric research offers quantitative insights, whereas content analysis provides the qualitative evaluation of the literature.

Findings
The findings suggest that recent research in this area focuses on exploring the role of women entrepreneurs and social entrepreneurship in the family business. The literature also finds that because of the nature of family businesses, successors get the opportunity to use their family’s network, social status, financing and opportunity toward a well-developed market.

Research limitations/implications
This paper may help researchers and practitioners to identify the past and current research trends related to family business and entrepreneurship.

Originality/value
The concepts from network theory are applied for content analysis to identify and explore various family business and entrepreneurship literature sub-domains.
Usage of smartphone for financial transactions: from the consumer privacy perspective
Authors: Chatterjee Sheshadri , Chaudhuri Ranjan , Vrontis Demetris , Hussain Zahid 
Published: October 2021

PurposeThis study aims to empirically examine how consumer privacy concerns (CPC) impact smartphone usage for financial transactions. The study also investigates the moderating impact of regulations on this action.Design/methodology/approachWith the inputs from literature and related privacy theories, a theoretical model was developed. The model was later empirically validated using the partial least squares structural equation modeling technique with 367 respondents from India.FindingsThe study finds that CPC significantly impacts on consumer behavior in using smartphones for financial transactions. The study also highlights that regulation has a moderating impact on consumer usage of smartphones for financial transactions.Research limitations/implicationsThis study provides valuable inputs to smartphone service providers, practitioners, regulatory authorities and policymakers on appropriate and secure usage of smartphones by consumers, ensuring privacy protection while making financial transactions.Originality/valueThis study provides a unique model showing the antecedents of CPC to impact the behavioral reaction of smartphone users mediated through the ingredients of privacy calculus theory. Besides, this study analyzes the moderating effects of regulation on the use of smartphones for financial transactions. This is also a novel approach of this study.
Support of young children from disadvantaged families in Switzerland: mixed-method evaluation of the home visitation program Schritt:weise
Authors: Lannen Patricia , Duss Isabelle 
Published: October 2021

Purpose
Children from disadvantaged families often already show developmental deficits at the time of school entry. The goal of Schritt:weise, a preventive, easy-access early education program for children ages one to five is to avoid such deficits through a combination of home visits by semi-professionals as well as center-based group activities. The program has been translated and adapted from the Dutch program Opstapje and is now being implemented in different regions in Switzerland. To offer the program more readily in rural areas, four adapted models were developed. This paper aims to provide a mixed-method, combined process and outcome evaluation of these newly developed models.

Design/methodology/approach
For this combined process and outcome evaluation, standardized testing for child development outcomes and interviews with different stakeholders were conducted.

Findings
The evaluation found that implementation of all four models was feasible and children developed along the norm in all four of the models. This finding was confirmed one year after program completion.

Research limitations/implications
Building an evidence-base to better understand success of early intervention programs is key to effectively supporting children from disadvantaged families in their development.

Practical implications
This early education program using home-visitation with semi-professionals is feasible and successful in reaching children from disadvantaged families.

Social implications
It is possible to support children from disadvantaged families through the program Schritt:weise and prevent developmental deficits at school entry.

Originality/value
The finding that children developed along the norm in all four models means that the key program goal was achieved and that the specific implementation model can be chosen based on the specifics of the implementation context.
Host country employees’ negative perceptions of successive expatriate leadership: the role of leadership transference and implicit leadership theories
Authors: Ciuk Sylwia , Schedlitzki Doris 
Published: October 2021

PurposeDrawing on socio-cognitively orientated leadership studies, this paper aims to contribute to our understanding of host country employees’ (HCEs) negative perceptions of successive expatriate leadership by exploring how their memories of shared past experiences affect these perceptions. Contrary to previous work which tends to focus on HCEs’ attitudes towards individual expatriates, the authors shift attention to successive executive expatriate assignments within a single subsidiary.Design/methodology/approachThe paper is based on an intrinsic case study carried out in a Polish subsidiary of an American multinational pharmaceutical company which had been managed by four successive expatriate General Managers and one local executive. The authors draw on interview data with 40 HCEs. Twenty-one semi-structured interviews were conducted with staff who had been managed by at least three of the subsidiary’s expatriate leaders.FindingsThe authors demonstrate how transference triggered by past experiences with expatriate leaders as well as HCEs’ implicit leadership theories affect HCEs’ negative perceptions of expatriate leadership and lead to the emergence of expatriate leadership schema.Originality/valueTo the best of the authors’ knowledge, this is the first study that explores the role of transference and implicit leadership theories in HCEs’ perceptions of successive executive expatriate assignments. By focussing on retrospective accounts of HCEs who had been managed by a series of successive expatriate leaders, our study has generated a more nuanced and contextualised understanding of the role of HCEs’ shared past experiences in shaping their perceptions of expatriate leadership. The authors propose a new concept – expatriate leadership schema – which describes HCEs’ cognitive structures, developed during past experiences with successive expatriate leaders, which specify what HCEs believe expatriate leadership to look like and what they expect from it.
Risk sharing vs risk shifting: a comparative study of Islamic banks
Authors: Khandelwal Sunil , Aljifri Khaled 
Published: October 2021

Purpose
This study aims to compare the use of risk-sharing and risk-shifting contracts (RSFCs) in Islamic banks using a triple grouping of conservative, moderate and liberal Islamic banks based on the Khaled Khandelwal (KK) model. Six fundamental Islamic contracts are used in this study, namely, Mushãrakah, Mudãrabah, Murãbaha, Salam, Ijãrah, Istisnã. Mushãrakah and Mudãrabah represent profit and loss sharing contracts (i.e., risk-sharing contracts – RSHCs), whereas Murãbaha, Salam, Ijãrah and Istisnã represent RSFCs. This study extends the previous studies by addressing an issue that has been neglected in the literature. The extent to which the two groups of contracts are used is extremely important because of its effect on the valuation of Islamic banks and on their earning quality.

Design/methodology/approach
This study aims to analyze, using descriptive statistics and inferential statistics, the use of RSHCs and RSFCs made by 72 fully Islamic banks, using a sample that includes banks in most of the countries where Islamic banks are present. Only fully Islamic Banks were considered, that is, banks that are essentially mainstream banks; therefore, banks that include only a specific line of Islamic products, often called the Islamic Window, were excluded. The total number of the sample was 118, but the study was restricted to 72 banks due to the availability of time series data covering the period of study, 2007 to 2015.

Findings
The study documents that over the period 2007 to 2015 the moderate banks have better distribution and balance of RSHCs and RSFCs than the conservative and liberal banks. The conservative banks are found to depend greatly on RSFCs, whereas the liberal banks are found to depend almost completely on RSFCs. Unexpectedly, the conservative banks have not shown a noticeable improvement over the period of analysis on their level of reliance on RSHCs. The results show that there is a significant difference in the percentage income distribution of the two contracts between the moderate banks and the conservative banks and between the moderate banks and the liberal banks. However, no significant difference was found between the conservative banks and the liberal banks.

Originality/value
The study uses an alternate rating model for Islamic financial institutions. The study examined the issue of risk sharing and risk shifting contracts usage in banks for a long period of nine years and at a global level and with an additional dimension of three categories of Islamic Banks based on the KK model.
Impact of gender diversity in boardroom on risk-return profile of Indian corporates
Authors: Nigam Narander Kumar , Singh Kirtivardhan , Arya Purushottam 
Published: October 2021

PurposeThe existing literature point that the presence of women directors in a firm reduces its risk. However, the relation between boardroom gender diversity and a firm’s return is widely disputed leading to no concrete answer. Some studies mention that women directors have a positive impact on firm performance, whereas, on the other hand, some findings suggest that women directors reduce financial performance. This paper aims to study the relationship of firm risk and return with boardroom gender diversity and the net impact on firm performance in the Indian context. This study uses not only traditional measures of risk and return but also the third measure of risk-adjusted returns to postulate its findings.Design/methodology/approachBased upon the data of the top 100 of the Bombay Stock Exchange-500 firms for the period FY 2009–2010 to FY 2018–2019, this study applied fixed effect panel regression and random effects Tobit regression to examine the effect of board gender diversity on firm performance.FindingsThe study concludes that firms with women directors on board have lower risk and lower returns. It also results in a higher risk-adjusted return, creating a positive impact on a firm’s performance.Originality/valueThe paper contributes to the existing literature on corporate governance by considering return, risk and risk-adjusted returns in single research to have a holistic measure of firm performance. It provides empirical evidence from one of the largest emerging economies, India where the female director and independent female director have been introduced recently.