bluuebunny/crossref_metadata_2025_split huggingface dataset:
Protection of Pegagan (Centella asiatica) Extract Through Hsp60 and Bax Expression on Stunting Model Zebrafish Larvae (Danio rerio) By Rotenone Inducted
Authors: Wijayanti Aida Ratna , Ridlayanti Annisa , Muljohadi Ali Husnul Khotimah 
Published: February 2021

Abstract
Objective: Rotenone is a pesticide (insecticide and fish killers) that is widely used in water. This study utilised rotenone as a model to initiate the occurrence of stunting on zebrafish larvae. Rotenone acts to inhibit the mitochondrial complex I to form an Adenine Tri Phosphate (ATP), Centella asitica(CA) is commonly used as Ayurveda medication as the triterpene in CA’s ingredient and antioxidant protect cells from oxidative damage. This study aimed to know the effect of CA extract on the stunting model zebrafish larvae induced by rotenone through Hsp60 and Bax expression. Methods: Zebrafish embryos were exposed with rotenone [10 ppb] and CA extracts(concentration 2,5 µg/mL; 5 µg/mL and 10 µg/Ml) from the age of 2 to 72 hpf (hour post fertilization).The body length was measured at the age of 3, 6, and 9 dpf (day post fertilization) using Image Raster software. The measurement of Hsp60 and Bax expression used IHC method on wholemount zebrafish larvae through DAB colouring. Density integrated was observed with Image J software. Results: The results of the study showed, There was an increase in Hsp60 and Bax expression on the zebrafish larvae induced by rotenone. In addition, CA extract with concentration [5 µg/mL] increased the body length at the age of 9 dpf (p<0,05). The observation of Hsp60 and Bax expression showed there was a significant decline on the CA extract groups with concentration [5 µg/mL]. Conclusion: CA extracts with concentration [5 µg/mL] increase the body length of zebrafish larvae and decreased Hsp60 and Bax expression.
The Formulation and Physical Evaluation of Emulgel the Kalakai (Stenochlaena Palustris Bedd) Roots Ethanol Extract As a Sunscreen
Authors: Paramawidhita Risqika Yuliatantri , Adawiyah Rabiatul , Umaternate Agustinawati 
Published: February 2021

Abstract
Kalakai (Stenochlaena palustris) is one of the diversity of Kalimantan typical plants that are often consumed as food but can also be used as medicinal plants. Ethanol Extract of Kalakai Root has been shown to have very strong antioxidants and has an SPF value of extreme ability level. Researchers want to examine a form of cosmetic emulgel which potentially as sunscreen. The purpose of this study is as a preliminary stage to see how the physical evaluation results (organoleptic, pH and dispersal power) of emulgel kalakai roots ethanol extract at 1% and 2% concentration. The results obtained for formula I (1%) and formula II (2%) of emulgel kalakai roots ethanol extract meet the organolepic test quality parameters, pH and spreadability test.
Simplex Lattice Design Analysis for Optimizing the Sangketan Extract (Achyranthes aspera) production
Authors: Fanani Zaenal , Setyowati Endang , Etikasari Ria 
Published: February 2021

Abstract
The need for medicinal raw materials is increasing, in line with the increasing of traditional medicine utilization. Sangketan (Achyranthes aspera) is one of the potential medicinal plants, considering this plant contains active compounds that are beneficial for health. In an effort to increase productivity, quality, and continuity of medicinal plants, improvement in cultivation techniques is needed. The right use of growing media, will provide optimal environmental conditions for plant growth. Animal manure and rice husk charcoal are organic material, that can be used as a mixture of growing media. The purpose of this study was to determine the optimal combination of growing media, that could support the extract production from Sangketan. In Sangketan cultivation, given treatment at growing media composition of soil + rice husk charcoal, with comparison 1:1; 1:2 and 2:1. Whereas the fertigation uses goat manure fertilizer, with a concentration of 1 kg per 5 liters of water, dose of 60 ml per plant every two weeks. Sangketan is harvested after 4-5 months, then quantitative analysis is performed of resulting extracts. The research data was applied using simplex lattice design (SLD), to obtain the optimal combination of growing media. Sangketan resulting extracts was applied using SLD, obtained the equation Y = 8.94 (Charcoal) + 11.585 (Soil) + 14.26 (Charcoal.Soil). Results of this study can identify and evaluate, technologies that support the sangketan development as a raw material for traditional medicine.
Effect of Boiling Time on Content of The Total Flavonoid of Kitolod (Isotoma longiflora (L.) C. Presl.)
Authors: Arrosyid Muchson , Mustofa Choiril Hana , Sutaryono  , Rohmah Alifiasri Praptiwi 
Published: February 2021

Abstract
Kitolod (Isotoma longiflora (L) C. Persl) is a annual herbaceous plant that usually grows on the edge of a moist wall. The leaves of the kitolod plant can be used as medicine for healing wounds, asthma, bronchitis, rheumatism and so on. Kitolod contains chemical compounds namely alkaloids namely lobelamin, isotomine and lobelin. In the leaf part of kitolod contains chemical compounds namely alkaloids, saponins, polyphenols and flavonoids. This study aims to determine the effect of boiling time on the total flavonoid content of water extract of kitolod leaves. Extraction was done by boiling, the determination of the total amount of flavonoids was carried out by the AlCl3 method and then the absorption was measured using Spectrophotometry UV-Vis with a wavelength of 431 nm. The results showed that qualitatively the samples contained flavonoids by showing red. Quantitatively the kitolod leaves are boiled with longer time, the total flavonoid content will decrease. The total flavonoid content of kitolod leaves in boiling 5 minutes obtained an average level of 0.31995% w/w or 31.9 mg/kg, boiling 10 minutes 0.25635% w/w or 25.6 mg/kg, boiling 15 minutes 0.2239% w/w or 22.3 mg/kg, boiling 20 minutes 0.17254% w/w or 17.2 mg/kg.
Improving plastic degradation by increasing the thermostability of a whole cell biocatalyst with LC-cutinase activity
Authors: Moeis Maelita Ramdani , Maulana Muhammad Farhan 
Published: February 2021

Abstract
Global consumption of polyethylene terephthalate (PET) increases each year, resulting in considerable buildup of plastic waste in the environment. A whole cell biocatalyst (WBC) with LC-cutinase bound to its outer membrane had been constructed to hydrolyze PET (optimum temperature 55°C). The aim of this study was to improve WBC viability at 55°C by inserting Ef-TU gene from sugarcane into WCB cells, with the hope of improving its hydrolytic activity. Escherichia coli BL21(DE3) was co-transformed with two plasmids, the first contained Lpp-OmpA-LC-cutinase fused gene and the second contained Ef-TU gene. Cells transformed with only the first plasmid were used as control. The cells were grown at 37°C and 55°C and viability was analyzed by total plate count. LC-cutinase activity was measured using pNPB as substrate and its capability to hydrolyze PET was observed by scanning electron microscopy. The presence of Ef-TU improved WCB viability at 55°C after 90 minutes incubation and LC-cutinase activity remained stable after 72 hours incubation at 55°C. LC-cutinase activity of WCB with Ef-TU was consistently higher than without EF-TU. Scanning electron micrograph of PET sheets incubated with WBC cultures with Ef-TU showed larger pockets than without EF-TU.
Method validation of As, Cd, Cr, Cu, Mn, Ni, Se, and Zn metals in Citarum River sediment using Inductively Coupled Plasma – Optical Emission Spectroscopy (ICP-OES)
Authors: Koesmawati T A , Tanuwidjaja S , Nurachman A 
Published: February 2021

Abstract
The Citarum River has a volcanic catchment area in West Java Province. It is also one of the nationally strategic rivers in tropical Indonesia due to its roles in water supply and in power generation. However, the Citarum River has experienced a decline in quality in which one of the causes is pollutants containing heavy metals. This research is aimed to validate the ICP-OES method in analyzing metals in sediment. Several parameters have been taken into account and evaluated for the validation of method, namely: linearity, the minimum detection limit, the limit of quantification, accuracy, and precision. The linearity of As, Cd, Cr, Cu, Mn, Ni, Se, and Zn was found satisfactory with coefficient of correlation (r) > 0.9995. The precision of all metals was calculated as relative standard deviation (RSD) and was found that the RSD value was < 2/3 CV Horwitz. The recovery value of all metals was found between 80-95%. The limit of detection (LOD) value for all metals was below 10 ug/L except for Zn and Mn. The method was validated using CRM PACS-3. Metal concentrations in the Citarum River sediment for all stations after being compared with the quality standard of ANZECC are still below the threshold values.
Optimization Process In The Synthesis of Stannous Chloride (SnCl2) by Redox Method In The Context of Downstream Tin Derivative Product
Authors: Wulandari Nurul , Wismogroho A S , Widayatno W B , Amal M I , Kusuma D Y 
Published: February 2021

Abstract
Indonesia has been known as the Tin Island in Southeast Asian Tin Belt region. This makes Indonesia listed as the second biggest tin producer in the world after China, with production reached 33.444 metric tons. However, 95% of tin which has been exported is still in the form of bars, not the optimal downstream tin derivative products, especially in the form of tin chemicals such as SnCl2. It turned out that tin chemical products are imported to meet the needs of domestic industry. In 2014, tin import reached USD 200 million. As one of the downstream products of tin derivatives, SnCl2 can be used as a catalyst, reducing agent, and as the main ingredient for other tin derivative products. This research was conducted based on the demand of industry, PT Timah Industri, to study the synthesis of SnCl2 powder in optimum conditions with redox method. The tin powder was dissolved in concentrated HCl heated on a hotplate, with variations in the particle size of the tin, HCl concentration and the reaction temperature. Differential Thermal Analysis (DTA) was carried out at 8000C with a heating rate of 100Cmin-1. The morphological structure of SnCl2 powder can be determined using 3D-Optical Microscope VHX-5000. As for the SnCl2 phase, it can be identified using the Rigaku SmartLab X-ray diffraction. This attempt has succeeded in synthesizing SnCl2 powder at optimum conditions with a yield percentage of 95%.
Analysis Of Organoleptic And Coliform Value In Fresh Mackerel (Rastrelliger Sp.) Fish In Tpi Sorong City
Authors: Sukmawati Sukmawati , Badaruddin Iksan , Dewi Nurul Kusuma , Situmorang Nurbaity , Mahfut Mahfut , Mustapa Fery 
Published: February 2021

Abstract
Fish is one of the fishery food ingredients needed by humans because in fish meat there are compounds that are needed by the body which consists of protein, fat, carbohydrates, vitamins and mineral salts. The purpose of the study was to determine the quality of fresh mackerel (Rastrelliger sp.) based on organoleptic test results, and to determine the content of fresh mackerel (Rastrelliger sp.) Coliform at the auction place of Sorong city castle bridge fish. The research method that has been used are experimental method using a complete randomized design analysis, and descriptive method that describes the Coliform bacteria in fresh fish products. Organoleptic quality value of eye condition, gill condition, texture, meat, smell mackerel at TPI Sorong city showed an influence on the storage time given. While the total coliform estimation test results on sample A, sample B, sample C, sample D and sample E in the first 3 hours and 6 hours following treatment showed that the highest total coliform was 11 MPN/g index and the lowest was 2.4 MPN/g. The conclution of organoleptic quality of fresh mackerel in the Sorong city TPI of the 5 samples showed an influence on the quality of the eye, gill, texture, fish meat and smell on the time treatment given at the α95% confidence level. While the total coliform in the first 3 hours of the 5 samples there are 4 samples (Samples A, B, C, and D) have total coliform bacteria not exceeding the maximum SNI limit.
Re-Food: Digital Platform-based Innovation Solutions for National Food Waste Problems
Authors: Pramana Rafi Yoga , Fadillah Achmad , Daryanto Arief 
Published: February 2021

Abstract
The problem of food waste in Indonesia is an important issue to overcome. Indonesia is the second-largest producer of food waste in the World. This makes national food waste issues get more attention. This paper aims to identify problems in food waste prevention, to formulate prototype of food waste application, to design digital platform-based business models innovation that complies with consumer preferences, and to map the impact generated by the presence of Re-Food. The method used are the customer discovery method with a qualitative descriptive approach and business model innovation methods. The results of this paper are verified social enterprise business model innovation offering an application that can help in utilizing excess food and help in the social problems of hunger and poverty. The potential impact generated by the Re-Food business model is the opportunity to contribute to social problems by utilizing environmental issues and increasing education on environmental issues, especially in food waste issues.
Implementation of Mathematics Learning-Assisted Cabri 3D Software to Improve Spatial Ability of High School Students on Three Dimensional Geometry
Authors: Yuliardi R , Mahpudin A , Rosyid A 
Published: February 2021

AbstractThe purpose of this study to analyze student’s spatial abilities to resolve 3D geometry problems through mathematics learning-assisted using Cabri 3D software. The population in this study were students from one of High School Student in Kuningan, West Java. Selection of sample by purposed random sampling, the experimental class is taught mathematics learning-assisted using Cabri 3D, while the control class is taught by conventional learning. This study was quasi-experimental with pretest and posttest control group design. Data analysis using two way anova by linking between learning model with gender factor and early mathematical abilities (KAM). Based on the results; (1) The enhancement of student’s spatial abilities through Cabri 3D was higher than the conventional learning; (2) based on gender, there were no significant effect of student’s spatial abilities who exposed with Cabri 3D and conventional learning; (3) based on KAM, there was significant effect of student’s spatial abilities among ability of high, middle, and low KAM. The differences occur between high, middle and low KAM. (4) based on the questionnaire results, students ‘responses to the positive Cabri 3D positive model, as much as 73.85% agree that learning Cabri 3D can improve understanding, interest and students’ abilities to learn the concept of 3 dimensional geometry. Based on this result, mathematics learning-assisted Cabri 3D can be applied in the process of mathematics learning in high School.
Analysis of Raw Material Inventory Planning Considering Uncertainty Demands (Case Study: Model Q with Back Order at PT. X)
Authors: Dewi Nurlaela Kumala , Andriant Irayanti , Loren Julia 
Published: February 2021

Abstract
In the production process a variety of products owned by PT. X are very high because the company does not have the same product to make every day. In the delivery of raw materials by suppliers there is also a delay in delivery caused by the empty stock of raw materials at the supplier and also the pre-order purchasing system. Problems with PT. X are planning demand and raw material inventory by considering demand uncertainly. As a first step in choosing the right inventory policy method, the Kolmogorov-Smirnov Test for normality test and ADI (Average Demand Interval) test are the classification of raw materials based on demand patterns. Of the five types of raw materials, the ADI value is 0, 91 and 1, which means < 1, 32. So that the completion of raw material inventory planning is done by probabilistic inventory policy method Continuous Review (Q) Back Order with Hadley - Within algorithm. The results of data processing are known to be the level of service of each type of raw material with the calculation of the proposed method, the average value of the service level for the five types of raw materials studied reaches 99, 30%. Where the probabilistic method of the Continuous Review System (Q) Hadley-Within algorithm, provides savings of 7,75% of the total cost of the existing inventory of the company.
Analysis of contrast limited adaptive histogram equalization (CLAHE) parameters on finger knuckle print identification
Authors: Hana F M , Maulida I D 
Published: February 2021

Abstract
Recently, safe and reliable identification systems are strongly needed. One of them is Biometric Identification System. A Biometric characterization of Finger Knuckle Print ( FKP) that a person has is unique.A method to improve image enhancement is needed in order to produce a better and clearer image. Contrast Limited Adaptive Histogram Equalization (CLAHE) is an image enhancement method that can provide clip limit and region size. This research proves that changes in the region size and clip limit parameters can affect the accuracy of the finger knuckle print biometric identification by extracting Speeded-Up Robust Features (SURF) and PrincipalComponentAnalysis (PCA) features. The highest accuracy using SURF extraction is obtained at region size 5x5 and clip limit 0.14 which is 97%, while the highest accuracy with PCA feature extraction is obtained when the region size is 2x2 and clip limit is 0.12, 0.13, 0.14, 0.15, 0.18, 0.2, 0.4, and 0.8 which is 91%.
Transforming the Baitul Maal Wal Tamwil Business Model through the Patform Design Model
Authors: Latifah Fitri Nur , Masruchin  , Wardhana Bayu , Retno Eris Dwi , Monika Lela 
Published: February 2021

Abstract
Digitalization in Islamic financial sector is a demand that cannot be denied in the development of the current technological era. We realized that most of Islamic financial institutions are not able to apply technology in their daily activities. There are many Baitul Maal wa Tamwil (BMT) that have not yet supported with technology in their business model. This study attempts to conduct research on the application of technology at this BMT by using a canvas model design platform. Business model canvas is a concept used to describe the business model of a business that developed by ostelwalder and pigneur, with the aim that everyone has the same perception of the business model. Within the business model canvas there are nine elements that represent content in a business i.e. key partners, key activities, value propositions, customer relationships, customer segments, key resources, channels, cost structures, revenue streams.
Computerized Text Analysis on Self-Description Text to Get Student’s Prevailing, Confidence, and Drives
Authors: Syah TA , Nurhayaty A , Apriyanto S 
Published: February 2021

Abstract
Using a sample of 26 participants, we asked the students to prepare a text in written self-description at the end of the final path presentation in the sixth semester. One interesting thing is the relationship between personality differences and the types of words that people use. Recent studies covers an observing the relationship between personal qualities and the use of language in creating self-description. Text files are organized for transcription LIWC analysis to calculate the percentage of clout, authentic, and drives that student’s use. The results supported this possibility: LIWC’s clout, authentic, and drives dictionaries can be used as indicators of the student’s prevailing, confidence, motives and needs, we found that when calculated on written text, LIWC scores were connected with language dimension and psychology issues. These findings recommend that LIWC clout, authentic, motives, and needs dictionaries possibly will catch self-reported subjective emotion experience when applied to everyday activities. To enrich students’ confidence, power, motives, and needs toward the psychology issues faced by the students, students should be stimulated to discover the field of psychological processes and linguistic patterns by detecting their emotional and perceptual experiences.
Analysis and Design of Web-Based Health Service Information Systems (E-Health), in the Industrial Revolution Era 4.0
Authors: Purnomo Wulan Andang , Prima Wahyu , Yusran  , Efendi Raimon , Suryadimal  
Published: February 2021

AbstractThis research is motivated by an analysis of the need for a health service information system which is an important part that cannot be separated from the health system in a country. This study aims to provide fast service information to the public and can increase the role of health care institutions through the provision of information about health complaints that occur in the surrounding environment. Especially in the era of the industrial revolution 4.0 which is also known as the digital era, where many sectors of life are inseparable from the participation and use of computer technology. Community Health Centres (Puskesmas) is one of the health service centers at the sub-district level. The number of patients is quite large, causing problems in getting information about the patient, the number of patients, the number of visits, total income, data on drug supply and drug data that has been used by the puskesmas. Based on the existing problems, it is necessary to design a web-based health service information system using the PHP and HTML programming languages and MySQL as its storage media or database. The system development methodology that I use is the prototype methodology. The result of the development of the system is that the system can help the Public Health Service Center to manage existing health services, especially helping the community to obtain important information about health services.
M-Bonk Based E-Participation in Street Infrastructure Development
Authors: Abadi Totok Wahyu , Putra Wahyu Pratama , Choiriyah Ilmi Usrotin , Riyadh Umar B A. 
Published: February 2021

Abstract
M-Bonk is an Android-based e-participation media used by local governments to increase community participation in reporting road damage in Sidoarjo Regency. This explanation approach study aims to analyze and explain self-efficacy, assertiveness, acceptance of M-BONK application users, socialization of their use, and their effects on community participation in the development of road infrastructure in Sidoarjo Regency. The place of this study was in Sidoarjo Regency using a sample of 91 M-Bonk application users as respondents and eight informants. Analyzing techniques used for quantitative data are multiple linear regression and interactive analysis for qualitative data. The results showed that self- efficacy, assertiveness, socialization, and acceptance affected community participation in road infrastructure development. The influence of these four variables on community participation was 97.4%. The novelty of this research is, first, the use of information communication technology applications provides the value of benefits, convenience, and increased public trust and desire to apply it as a medium to participate in development. Second, the community participates in development because they feel confident in their ability to participate in it; have the courage to express feelings, expressions, and decisions to be involved in infrastructure development. Third, socialization is an important process between the government and he community in effective development interactions. Fourth, e-community participation in development has different levels. The levels of e-participation consist of e-information, e-consultation, e-dialogue, e-collaboration, and e-empowerment.