bluuebunny/crossref_metadata_2025_split huggingface dataset:
Detection of CTX-M Gene in Escherichia coli Producing Extended Spectrum Beta Lactamase (ESBL) Isolated from Patients with Urinary Tract Infection
Authors: Pestariati Dr. , Suhariyadi Dr. 
Published: September 2021

Escherichia coli is a Gram-negative bacterium from Enterobacteriaceae family causes urinary tract infections (UTI). A major problem encountered in antibiotics therapy is Multiple Drug Resistant Organisms (MDROs). MDROs occur because of the presence of resistance coding genes such as CTX-M which causes bacteria to produce the Extended Spectrum Beta-Lactamase (ESBL) enzyme. This study aims to detect the presence of the CTX-M gene in Extended-Spectrum Beta Lactamase (ESBL) Escherichia coli isolated from UTI patients. The study was conducted at the Institute of Tropical Disease (ITD) Airlangga University, Jl. Mulyorejo Campus C Surabaya, Indonesia for polymerase chain reaction (PCR) examination. The isolation and identification of Escherichia coli bacteria were carried out at the Microbiology Laboratory Department in Poltekkes Kemenkes Surabaya, Jl. Karangmenjangan 18A, Surabaya, Indonesia. Conventional identification of Escherichia coli and the presence of the CTX-M gene were observed using the PCR method. The results showed that 18 of 30 samples (60%) were caused by Escherichia coli. Escherichia coli producing ESBL was found in 15 samples (83%), of which 12 samples (80%) showed the presence of the CTX-M gene.
Keywords : CTX-M gene, Extended-Spectrum Beta Lactamase (ESBL), Escherichia coli
​Effect of Different Growing Conditions and Genotypes on Growth and Bulb Parameters of Asiatic Lily
Authors: . Savita , Chahal Devender , Malik Arvind , Devi Sarita 
Published: September 2021

Background: The present investigation was carried out during the cropping season of year 2019-20 to find out the most suitable growing condition as well as variety of Asiatic lily for the production of bulbs/bulblets under Hisar (Haryana) conditions. Methods: The experiment was laid out in RBD (factorial) with three replications and twelve treatment combinations, comprising of three growing conditions (polyhouse of 200 µ, green shade-net of 50% shade and open field) and four varieties (Courier White, Tresor, Nova Lux and Red Stone). Result: These varieties of Asiatic lily when grown under polyhouse performed better in some parameters resulting in early bulb sprouting, maximum plant height and number of leaves/plant and the minimum days upto harvesting of bulbs, however, in other parameters viz. leaf length and width, weight of bulbs/plant, diameter of bulb and number of bulblets/plant, the shade-net condition was observed to be better than polyhouse. Overall, Tresor performed better, resulting in early bulb sprouting (5.11 days), minimum days (236.56) upto harvesting of bulbs and the maximum plant height (89.01 cm), number of leaves/plant (64.08), leaf length (9.50 cm), diameter of bulb (3.91 cm) and number of bulblets/plant (2.99) and it was followed by Red Stone which produced maximum weight of bulbs/plant (59.03 g) and observed next to Tresor in most of the parameters. Tresor grown under polyhouse took minimum days (231) upto the harvesting of bulbs, produced maximum plant height (115.13 cm) and number of leaves/plant (69.67) and it was closely followed by Red Stone grown under same condition. Further, under the shade-net, Red Stone produced maximum weight of bulbs/plant (65.30 g) followed by Tresor (63.93 g), while the maximum number of bulblets/plant (3.90) were produced by Tresor followed by Red Stone (3.44). Henceforth, Tresor and Red Stone when grown under polyhouse performed better in vegetative parameters and when grown under shade-net performed better in bulb parameters than the other combinations of growing conditions and varieties.
Assessment of Morphological, Genetical and Diversity Studies in Landraces of Rice (Oryza sativa L.)
Authors: . Sujata , Jekkaral Shridevi A. , Kumar B.M. Dushyantha , Gangaprasad S. , Halingali Basavaraj I. 
Published: September 2021

Background: Landraces displayed a wide range of genetic diversity in rice farming system. Landraces or local types have been used as the sources for the characters such as resistance to pest, disease, abiotic stresses and genetic sources for some physicochemical characters. Various types of landraces of rice are available in the major rice growing regions of Karnataka and hence, their characterization and establishment in uniformity is essential, to consider them as variety and can be protected under PPV and FR act. Investigation was undertaken to assess genetic variability and diversity in fifty one landraces of rice. Methods: The materials comprised of fifty one landraces of rice collected from Zonal Agricultural and Horticultural Research Station, Brahmavar and the experiment was conducted in randomized complete block design with two replications, following spacing of 20 cm between rows and 15 cm between plants at College of Agriculture, Shivamogga, during the Kharif 2018. Result: The present experiment will be useful for the farmers and researchers to identify the valuable germplasm for utilization in rice yield improvement and seek protection under Protection of Plant Varieties and Farmers’ Rights Act and also for protection of rice landrace like Gulvadisanna and Kayame registration made under PPV and FRA.
Factors Affecting Morbidity and Mortality in Gastrectomy Cases with Splenectomy
Authors: KALAYCI Tolga , İLİKLERDEN Ümit Haluk , KOTAN Çetin 
Published: September 2021

Gastric cancer is a worldwide health problem. The addition of splenectomy to gastrectomy is a controversial issue, especially
in cases located on the greater curvature. In this study, it is aimed to find the factors affecting morbidity and mortality in gastrectomy
cases with splenectomy. Patients who underwent surgery for gastric cancer in Van Yuzuncu Yil University, Department of General
Surgery between January 2010 and January 2018 were retrospectively selected. Splenectomy cases were filtered out in all gastrectomy
patients. Preoperative, intraoperative and postoperative data of the patients were collected. The effects of the collected parameters on
morbidity and mortality were evaluated. A p value of less than 0.05 was considered statistically significant. 45 patients were included
in the study. The mean age of all patients was 64.2 years (32-85) and the male to female ratio was 27/18. Thirty seven cases (82.2%)
were operated under elective conditions. The most common tumor location was cardia (n=24, 53.3%) and the most common surgical
method (n=39) was total gastrectomy (86.7%). Morbidity and mortality rate of the study were 46.6% (n=21) and 17.8% (n=8),
respectively. Mortality increased in patients who were operated in emergency conditions and received neoadjuvant therapy, p=0.002
and P=0.044, respectively. While surgery under emergency conditions increased mortality, preoperative neoadjuvant treatment
decreased mortality. However, there was no factor affecting morbidity. Splenectomy in gastric cancer surgery, if possible, should be
performed under elective conditions and after neoadjuvant therapy to reduce mortality
Difficulties in using correct English prepositions among EFL students
Authors: Abdalla Ibrahim 
Published: September 2021

The objectives of this study are to find out the reasons of this problem and find some solutions. The participants were ten students at levels seven and eight, at the Faculty of Sciences and Arts. The researcher used the Descriptive Statistical Method and collected the data via a written test as the main research tool. The data were then analyzed using a Statistical Package of Social Sciences Programme (SPSS). The results were as follows: Saudi students faced problems with using the correct prepositions after certain verbs. Students encountered difficulties in identifying the correct prepositional phrase. Students experienced the same problem with the use of English prepositions in writing tasks and in daily communication. The recommendations of this study are that students need to revise the use of English prepositions in general and need more practice in the use of prepositions after certain verbs. They also need to make a greater effort to use English prepositional phrases in their written work. Regarding writing courses, students need to focus more on the use of prepositions in general. Curriculum designers need more attention about teaching prepositions in their textbooks and curriculums.
HIGHLIGHTS:

Saudi students faced problems with using the correct prepositions after certain verbs.
Students encountered difficulties in identifying the correct prepositional phrase.
Students experienced the same problem with the use of English prepositions in writing tasks and in daily communication.
Rapid and inexpensive method of studying dedifferentiation in rabbit articular chondrocytes
Authors: Shah Fahad Hassan , Eom Young Seok , Ko Byung Su , Han Yohan , Kim Song Ja 
Published: September 2021

Abstract
Chondrocyte dedifferentiation is a cellular phenomenon in which chondrocyte loses its chondrogenic characteristics and ability to synthesize extracellular matrix. These conditions commonly reported in different cartilage degenerative diseases. The methods used to induce dedifferentiation in cell models of chondrocytes are often time consuming or require expensive reagents. Here in our protocol, we describe the utility of using 2-deoxy-D-glucose as a dedifferentiating agent for rabbit chondrocytes for studying dedifferentiation process.
Deep Brain Stimulation for Generalized Dystonia from Secondary Carnitine Deficiency: A Case Report and Literature Review.
Authors: Zhang Yixuan , Sperry Laura , Chan Michelle , Shankar Suma , Malhado-Chang Norika , O'Connor Kevin , Girgis Fady , Farias Sarah , Wheelock Vicki Lynn , Shahlaie Kiarash , Zhang Lin 
Published: September 2021

Abstract
BACKGROUND:

Carnitine deficiencies result from a metabolic disorder of fatty acid β-oxidation and may lead to organic acidemia, which are thought to be associated with dystonia, epilepsy, autism and developmental delay. Pharmacotherapy has been the dominant therapy, while many refractory patients still require other treatment. Deep brain stimulation (DBS) of the globus pallidus internus (GPi) has been found to be effective for medically refractory primary dystonia and now it has been proposed to be used for secondary dystonia from mitochondrial metabolic disorder.
OBJECTIVE:

To investigate the efficacy and safety of DBS treatment in secondary dystonia from organic acid metabolic disorder.
METHODS:

We present a patient born with secondary carnitine deficiency who had the onset of generalized seizures at age 4.5 months and developed torsion dystonia at age 14. Multiple medical therapies failed to adequately control her symptoms, therefore she received GPi DBS at age 26 years. In addition, we performed a literature review of this therapy in the treatment of organic acid metabolic disorder.
RESULTS:

Our patient’s dystonia resolved without side effects post-DBS surgery, but intermittent spastic symptoms along with severe pain in her lower extremity persist. Concerning the 8 cases from our literature review, 7 received GPi DBS, and had improvement in motor symptoms. Overall, DBS efficacy was lower than in treatment of primary dystonia. One patient with methylmalonic acidemia received STN DBS and had marked improvement in dystonia and reduction in pain afterwards.
CONCLUSION:

DBS has become an effective therapy in refractory secondary dystonia from organic acid metabolic disorder. More prospective studies are needed to determine the eligibility and efficacy of this surgical therapy in these cases.
Does Obesity Change The Perception of Pelvic Organ Prolapse?
Authors: Friedman Talia , Dietz Hans Peter 
Published: September 2021

Abstract
Introduction and hypothesis: There is a complex relationship between obesity and pelvic organ prolapse. The objective of our study was to evaluate whether body mass index (BMI) changes the relationship between signs and symptoms of prolapse.Methods This was a retrospective observational study based on datasets of women seen in a tertiary urogynecological centre with symptoms of pelvic floor and lower urinary tract dysfunction between April 2012 and October 2015. Patients underwent a strucrured interview, clinical examination (based on the POP-Q) and 4D transperineal ultrasound (TPUS). Offline assessment of sonographic organ descent was undertaken later, blinded to all patient data. Results: Weight and BMI affect the relationship between symptoms and signs of prolapse. This effect is statistically highly significant. The more obese a patient is, the less likely she is to notice a given degree of objective prolapse, regardless of whether it is diagnosed clinically or on imaging.Conclusions In this retrospective study, we found a highly significant effect of weight and BMI on prolapse perception in the sense that obesity seems to mask prolapse while a low BMI increases the likelihood of a given degree of prolapse being noticed by the patient.
Comparison of Agro-Wastes For Dewatering Enhancement For Dredged Sludge From Sewers
Authors: Nguyen Xuan Huan , Pham Thi Thuy , Dang Thi Thanh Huyen , Pham Thi Ngoc Lan , Tran Thuy Anh , Tran Thi Huyen Nga , Hoang Duc Quyen , Nguyen Manh Khai 
Published: September 2021

Abstract
With the increasing wastewater generation from domestic and industrial activities due to the rapid economic development, the generation of sludge, in particular dredged sludge from municipal sewer system, has been an issue in developing countries. This study evaluated the enhancement of sewer’s sludge dewatering via mixing the sludge with different agro-wastes, including corn core powder, rice husk powder, bagasse powder and peanut shell powder. The addition of these agro-waste powders helped decrease the sludge’s moisture contents up to 17% after mixing with the ratio of 1:3, 1:5 or 1:7. Statistical analysis revealed the impacts of both additive types and mixing ratio on moisture content reduction. Among the four types of agro-waste, rice husk was shown to be the best additive to dredge sludges with highest reduction of heavy metal concentration and moisture content. The addition of agro-waste powders to enhance the dewatering of sludges is quite promising in the context of promoting waste reuse and energy saving.
Teaching the Technical Essentials of Direct Ophthalmoscopy Using a Portable Fundus Camera
Authors: Feng Weibo , Zou Yuxian , Li Yonghao , Shen Tao , Gao Ya , Lu Lin , Liu Bingqian 
Published: September 2021

Abstract
Aim: A portable fundus camera could allow the third person to observe the technique essentials of direct ophthalmoscopy from a real time screen. This study was designed to compare the proficiency of teaching direct ophthalmoscopy using a portable camera with conventional way in medical students. Methods: Medical students of fourth year were invited to participate the study. At baseline, the participants were taught fundoscopy with a conventional direct ophthalmoscope shortly. Then they were randomized to be taught the skill of fundoscopy either with a portable fundus camera or with a conventional direct ophthalmoscope as control for two days. Accuracy tests to match a subject’s fundus with one of the four photographs after examining an undilated eye using a direct ophthalmoscope were performed at baseline and end point. Accuracy test scores and self-reported confidence were compared between the two groups. Results: A total of 160 students participated the study, with 79 assigned to the intervention group, and 81 to the control group. All the students finished the study. At baseline, there was no difference in accuracy test score between the two groups. After two-day training session, the accuracy score improved in 26/79 (32.9%) students of intervention group versus 15/81 (18.5%) of the control group (p=0.037). At end point, a total of 39/79 (49.4%) students in the intervention groups versus 25/81 (30.9%) in the control group identified the correct fundus photograph (p=0.017). The confidence levels were significantly higher in the intervention group than the control group. Conclusions: Teaching direct ophthalmoscope using a portable fundus camera is associated with improved accuracy score and elevated confidence level in medical students when compared with conventional method.
Biofiltration of Methane Fugitive Emissions from Landfills Using Scum from Municipal Wastewater Treatment Plants (Wwtp) as Alternative Substrate
Authors: Schirmer Waldir Nagel , Stroparo Erivelton César , Capanema Marlon André , Mazur Douglas Luiz , Jucá José Fernando Thomé , Martins Kelly Geronazzo 
Published: September 2021

Abstract
Biofilters have been recognized as key technology in the mitigation of greenhouse gases (GHG) emitted by landfills. This study aimed to evaluate the methane (an important GHG) oxidation efficiencies of two experimental biofilters at the municipal landfill of Guarapuava (Brazil) under normal conditions (control column), just using landfill cover soil with low organic matter content, and improved, exploiting dried scum from municipal wastewater treatment plant (SWWTP) mixed with the cover soil (enriched column, with a high organic matter content). The influence of parameters such as the methane inlet loading rates (22 and 44 gCH4.m− 2.d− 1), temperatures, methane concentration in the raw biogas, carbon/nitrogen ratio and moisture content of the packing materials on the oxidation of methane was also evaluated during 25 campaigns. The campaigns with the lowest methane loading rates applied to the biofilters showed the best methane oxidation efficiencies (98.4% and 89.5% in the enriched and control columns, respectively) as compared to campaigns with a higher load (92.6% and 82.6% in the enriched and control columns, respectively). In addition to the loading rates, the methane oxidation efficiencies were highly influenced by the organic matter content and C/N ratio of the packing materials evaluated.
Efficacy Comparison of Trifocal Bone Transport Using Unilateral External Fixator for Femoral and Tibial Bone Defects Caused by Infection
Authors: Liu Kai , Liu Yanshi , Cai Feiyu , Fan Chenchen , Ren Peng , Yusufu Aihemaitijiang 
Published: September 2021

Abstract
Background: This study aimed to evaluate the clinical and functional outcomes of patients with critical femoral and tibial bone defects treated by trifocal bone transport using the Ilizarov method.Methods: In a retrospective comparative study, 39 patients treated for lower limb bone non-union with bone loss measuring between 6 and 14 cm were included. Depending on the location of bone transport, the patients were divided into the femur group (n =18) and tibia groups (n =21). The demographics data, intraoperative records, and postoperative outcomes were documented and compared between the two groups. At the last follow-up, the bone and functional outcomes were evaluated according to the criterion given by the Association for the Study and Application of the Method of the Ilizarov (ASAMI) and postoperative complications evaluated by Paley classification.Results: The average follow-up time was 26.1 months (range 17–34 months) since the unilateral external fixators were removed. The mean size of the bone defect was 8.3 cm in the femur group, and 7.5 cm in the tibia group. All bone defects were reconstructed successfully. The mean time in external fixation in the femur group was 334.4 days, and in the tibia group was 344.6 days. The external fixation index (EFI) measured 55.9 days/cm in the femur group and 65 days/cm in the tibia group. A statistically significant difference of bone grade was found between the two groups (excellent/good/fair/poor, 3/11/3/1 vs 2/13/4/2, P<0.05), as well as the function grade in two groups (excellent/good/fair/poor/failure, 3/14/1/0 vs 4/13/3/1, P<0.05). According to the ASAMI classification, the clinical and functional results in the femur group were better than in the tibia group. The complication rate of the two groups was 94.4% vs 76.2% (femur vs tibia). One femur and five tibias were performed additional surgery because of delayed union and axial deviation. Conclusions: The trifocal bone transport using the unilateral external fixator is a reliable treatment in the management of post-traumatic and post-infection lower limb bone defects (>6cm). In the comparison of the tibia, the trifocal bone transport treatment period of the femur was shorter, the functional recovery was better and the risk of minor complications was higher.
Paenibacillus Piscarius Sp. Nov., a Novel Nitrogen-Fixing Species Isolated from the Gut of the Armored Catfish Parotocinclus Maculicauda
Authors: silva Mariana Barbalho Farias da , Lemos Ericka Arregue , Vollú Renata E. , Abreu Fernanda , Rosado Alexandre S. , SELDIN LUCY 
Published: September 2021

Abstract
A gram-positive, nitrogen-fixing and endospore-forming strain, designated P121T, was isolated from the gut of the armored catfish (Parotocinclus maculicauda) and identified as a member of the genus Paenibacillus based on the sequences of the 16S rRNA encoding gene, rpoB, gyrB and nifH genes and phenotypic analyses. The most closely related species to strain P121T were Paenibacillus rhizoplanae DSM 103993T, Paenibacillus silagei DSM 101953T and Paenibacillus borealis DSM 13188T, with similarity values of 98.9%, 98.3% and 97.6%, respectively, based on 16S rRNA gene sequences. Genome sequencing revealed a genome size of 7,513,698 bp, DNA G + C content of 53.9 mol% and the presence of the structural nitrogenase encoding genes (nifK, nifD and nifH) necessary for nitrogen fixation. Digital DNA-DNA hybridization (dDDH) experiments and average nucleotide identity (ANI) analyses between strain P121T and the type strains of the closest species demonstrated that the highest values were below the thresholds of 70% dDDH (42.3% with P. borealis) and 95% ANI (84.28% with P. silagei) for bacterial species delineation, indicating that strain P121T represents a distinct species. Its major cellular fatty acid was anteiso-C15:0 (42.4%), and the major isoprenoid quinone was MK-7. Based on physiological, genomic, biochemical and chemotaxonomic characteristics, we propose that strain P121T represents a novel species for which the name Paenibacillus piscarius sp. nov. is proposed (type strain = DSM 25072 = LFB-Fiocruz 1636).
Men’s Experiences of Sex and Intimacy After Prostate Cancer Treatment In China: A Qualitative Study
Authors: Wang Tong , Cheng Hui-Lin , Wong Ping Kit Kitty , Dong Wen 
Published: September 2021

Abstract
Purpose While the existing knowledge base on the impact of prostate cancer (PC) and its treatment on sexuality and intimacy has been generated from Western populations, there is a lack of such evidence in the Asian context. This study aimed to explore men’s experiences of sex and intimacy after PC treatment in China. Methods This study adopted an interpretive descriptive design. Using purposive sampling, 13 PC patients were selected from a urology outpatient unit of a hospital in South China and proceeded with individual semi-structured telephone interviews. Each interview was transcribed verbatim and analyzed using constant comparison analysis. Results Four themes emerged from the interview data, including (a) encountering altered sexuality, (b) communication and sexual adjustments, (c) maintenance of quality intimate relationship, and (d) lack of sexual health support. Conclusions The findings revealed that PC treatment significantly impaired patients’ sexual functions, and their sexual health needs were mainly unmet by healthcare providers. There is a great need to design culturally relevant interventions to improve sexual health among this population.