bluuebunny/crossref_metadata_2025_split huggingface dataset:
Nucleophosmin mutations lead to abnormal, but reversible, nucleoli architecture and aggregate formation – Implications for NPM1-targeting therapies in AML
Authors: Grundy Martin , Lucken Kellie , Xing Xiaomeng , Simpson Eva L. , Bayyoomi Ahmed , Beckett Alison J. , Prior Ian A. , Booth Daniel G. , Seedhouse Claire H. 
Published: December 2024

AbstractMutations in theNPM1gene represent the most common (>30% of patients) genetic alteration in Acute Myeloid Leukaemia (AML) and results in the mis-localisation of the mutated NPM1 protein from a predominantly nucleolar localisation to a predominantly cytoplasmic distribution. Numerous studies of NPM1 mutated AML have focussed on the aberrant cytoplasmic localisation of the mutated protein but efforts to reverse this mis-localisation therapeutically have so far resulted in limited clinical benefit. More recently, attention has shifted towards the nucleus with studies showing that mutant NPM1 binds to specific chromatin regions, where it directly regulates oncogenic gene expression. Here, we use high resolution imaging to demonstrate that Nucleophosmin (NPM1) is critical for maintaining normal nucleoli architecture and specifically the integrity of the nucleoli rim. We report for the first time that NPM1 mutated cell lines and primary samples have aberrant nucleoli architecture and demonstrate that the abnormal nucleoli phenotype is reversible. We also report the novel finding that NPM1 mutated protein forms distinct aggregates in NPM1 mutated cells and characterise these for the first time. This work reveals how nucleolar organisation contributes to the molecular mechanisms underpinning NPM1 driven AML and reveals unexpected novel vulnerabilities to be exploited for therapeutic intervention.
Evaluation of subretinally delivered Cas9 ribonucleoproteins in murine and porcine animal models highlights key considerations for therapeutic translation of genetic medicines
Authors: Wei Spencer C. , Cantor Aaron J. , Walleshauser Jack , Mepani Rina , Melton Kory , Bans Ashil , Khekare Prachi , Gupta Suhani , Wang Jonathan , Soares Craig , Kiwan Radwan , Lee Jieun , McCawley Shannon , Jani Vihasi , Leong Weng In , Shahi Pawan K. , Chan Jean , Boivin Pierre , Otoupal Peter , Pattnaik Bikash R. , Gamm David M. , Saha Krishanu , Gowen Benjamin G. , Haak-Frendscho Mary , Janatpour Mary J. , Silverman Adam P. 
Published: December 2024

AbstractGenetic medicines, including CRISPR/Cas technologies, extend tremendous promise for addressing unmet medical need in inherited retinal disorders and other indications; however, there remain challenges for the development of therapeutics. Herein, we evaluate genome editing by engineered Cas9 ribonucleoproteins (eRNP) in vivo via subretinal administration using mouse and pig animal models. Subretinal administration of adenine base editor and double strand break-inducing Cas9 nuclease eRNPs mediate genome editing in both species. Editing occurs in retinal pigmented epithelium (RPE) and photoreceptor cells, with favorable tolerability in both species. Using transgenic reporter strains, we determine that editing primarily occurs close to the site of administration, within the bleb region associated with subretinal injection. Our results show that subretinal administration of eRNPs in mice mediates base editing of up to 12% of the total neural retina, with an average rate of 7% observed at the highest dose tested. In contrast, a substantially lower editing efficiency was observed in minipigs; even with direct quantification of only the treated region, a maximum base editing rate of 1.5%, with an average rate of <1%, was observed. Our data highlight the importance of species consideration in translational studies for genetic medicines targeting the eye and provide an example of a lack of translation between small and larger animal models in the context of subretinal administration of Cas9 eRNPs.
Discovery of neuronal cell types by pairing whole cell reconstructions with RNA expression profiles
Authors: Ferreira Tiago A. , Eddison Mark , Copeland Monique , Lay Mary , Tenshaw Emily , Weldon Monet , Schauder David , Olbris Donald J. , Rokicki Konrad , Spruston Nelson , Tillberg Paul W. , Korff Wyatt , Dudman Joshua T. ,   The MouseLight Project Team
Published: December 2024

AbstractEffective classification of neuronal cell types requires both molecular and morphological descriptors to be collectedin situat single cell resolution. However, current spatial transcriptomics techniques are not compatible with imaging workflows that successfully reconstruct the morphology of complete axonal projections. Here, we introduce a new methodology that combines tissue clearing, submicron whole-brain two photon imaging, and Expansion-Assisted Iterative FluorescenceIn SituHybridization (EASI-FISH) to assign molecular identities to fully reconstructed neurons in the mouse brain, which we call morphoFISH. We used morphoFISH to molecularly identify a previously unknown population of cingulate neurons projecting ipsilaterally to the dorsal striatum and contralaterally to higher-order thalamus. By pairing whole-brain morphometry, improved techniques for nucleic acid preservation and spatial gene expression, morphoFISH offers a quantitative solution for discovery of multimodal cell types and complements existing techniques for characterization of increasingly fine-grained cellular heterogeneity in brain circuits.
The dentate gyrus provides flexibility for efficient spatial navigation
Authors: Soldi Natalia , Benas Sabrina , Kropff Emilio , Schinder Alejandro F. , Piatti Verónica C. 
Published: December 2024

AbstractThe hippocampus plays a critical role in spatial navigation and declarative memory. The dentate gyrus is the neurogenic region of the hippocampal formation and it has long been implicated in the fine separation of similar contexts or close object locations. However, it is unclear how an accurate discrimination could be beneficial to a goal-guided behavior in a changing environment. Therefore, we used chemogenetic inhibition to study the role of the dentate gyrus in a goal-guided spatial navigation paradigm over a familiar but dynamic crossword maze. Mice were challenged to localize a novel reward location from alternative pathways in two versions of the task with particular configurations in each experimental day. In the simple task, the two optimal paths to the goal shared some segments in their trajectory. In a more complex task, optimal trajectories demanded completely different directions to the reward location. Overall, mice with chemogenetic inhibition of the dentate gyrus were able to learn all the routes regardless the complexity of the task, similarly to control animals. However, after having solved a first route in the complex task, mice with dentate gyrus inhibition displayed an impairment to efficiently navigate over the alternate path. Our results demonstrate a role of the dentate gyrus in cognitive flexibility required to reach a goal in a changing familiar environment.
How the two-tier cross-domain control influence contractor’s design behavior: a configurational analysis
Authors: Yan Ling , Wen Shuangshuang , Li Qing 
Published: December 2024

PurposeThe contractor’s design behavior in engineering procurement construction (EPC) mode plays a decisive role in controlling design risks, ensuring construction quality and improving project value in terms of project performance. Organizational control is an important way to intervene and strengthen organizational behavior. Reasonably arranging organizational control of the design projects in EPC mode will greatly affect the contractor’s design behavior choices and achieve project goals. There are few existing studies that focus on the contractor’s classification design behavior management from the perspective of two-tier cross-domain control of the design projects in EPC mode. To fill the knowledge gap, this study explores how the two-tier cross-domain control linkage effect affects the contractor’s design behavior choice of the design projects in EPC mode through a configuration analysis.Design/methodology/approachThis study selects five antecedent variables from the perspective of two-tier cross-domain control (including inter-organizational and intra-organizational controls) through literature review. This study collected 290 valid responses from the design projects in EPC mode practitioners through a questionnaire survey and analyzed the data using fuzzy-set qualitative comparative analysis.FindingsThe results of the necessity and sufficiency analysis indicate that (1) single control is not a necessary condition for contractor’s design behavior, but outcome control and social control play a more universal role in generating a contractor’s design behavior. (2) The four paths that affect the contractor’s design proactive behavior can be divided into two groups: one emphasizes the combined effect of three inter-organizational control, while the other highlights the considerable impact of synergistic inter- and intra-organizational control. (3) There are four paths that affect the contractor’s design compliance behavior. Compared with the organizational configuration of the contractor’s proactive design behavior, process control should be combined with bureaucratic control and clan control to play a role in the absence of outcome control and social control.Originality/valueThis study provides a more detailed explanation for how the linkage effect of the two-tier cross-domain control affects the contractor’s design behavior of the design projects in EPC mode. It broadens the application of positive organizational behavior and organizational control theory. In practice, this study provides a suitable control path for mutual benefit and a win-win situation between owners and contractors to promote the success of the design projects in EPC mode.
How and when generative AI use affects employee incremental and radical creativity: an empirical study in China
Authors: Zhang Xin , Yu Peng , Ma Liang 
Published: December 2024

PurposeThe potential of generative AI (GenAI) to stimulate employee creativity has received extensive attention from industry and academia. However, there is still limited research on strategically using GenAI to leverage its positive effects on employee creativity. This study aims to clarify the effects of different GenAI use purposes on employee creativity.Design/methodology/approachBased on self-determination theory, this study explores the effects of work-related and nonwork-related GenAI use on incremental and radical creativity through the mediator role of exploratory and exploitative learning and the boundary role of perceived ease of use. This study constructs a theoretical model and uses structural equation modeling to test the model by analyzing survey data from 330 employees.Findings(1) Work-related and nonwork-related GenAI use positively impacts incremental and radical creativity through exploratory and exploitative learning; (2) work-related GenAI use contributes more to exploitative learning than to exploratory learning, while nonwork-related GenAI use contributes more to exploratory learning than to exploitative learning; (3) exploitative learning has a stronger positive impact on incremental creativity, and exploratory learning has a stronger positive impact on radical creativity; (4) perceived ease of use weakens the positive effects of nonwork-related GenAI use on exploratory and exploitative learning.Originality/valueFirst, this study enriches employee creativity research by revealing the relationship between different GenAI use purposes and incremental and radical creativity. Second, this study enriches employee creativity research by revealing the mediating role of exploratory and exploitative learning between GenAI use and incremental and radical creativity. Finally, this study enriches GenAI use research by revealing the moderating role of perceived ease of use between GenAI use and employee learning.
Sustainable finance, herding behavior and risk aversion during market volatility
Authors: Gavrilakis Nektarios , Floros Christos 
Published: December 2024

PurposeWe investigate herding behavior and explore how risk aversion interacts with herding in a sample of selected sustainability indices. Furthermore, we evaluate volatility co-movements and dynamic and time-varying correlations of two notable indicators: the cross-sectional absolute deviation of returns (CSAD) and the risk aversion index (RAI). Moreover, we explore a spillover mechanism (in the short and long run) of risk aversion to sustainable investing.Design/methodology/approachOur study uses daily prices from 01/06/2012 to 15/07/2022 obtained from S&P Dow Jones Indices. We use autoregressive - Glosten, Jagannathan, and Runkle generalized autoregressive conditional heteroscedastic (AR(1)-GJR GARCH) methodologies to measure the impact of cross-sectional absolute deviation of returns (CSAD) and risk aversion (RAI) indicators on the conditional variance of selected sustainability indices. Furthermore, we employ dynamic conditional correlation with generalized autoregressive conditional heteroscedasticity, generalized autoregressive conditional heteroscedasticity generalized autoregressive conditional heteroscedasticity mixed data sampling and dynamic conditional correlation with mixed data sampling models to examine any dynamic correlation, spillover volatility effects and the predictability stance of the CSAD and the RAI factors on sustainable investing.FindingsOur empirical outcomes capture no-herding behavior but display herding on the risk aversion indicator. The cross-sectional dispersion of returns positively affects the conditional variance of all selected sustainable markets, besides emerging markets, while the risk aversion factor negatively influences the conditional variance for Europe and the USA. We have identified long-term contagion effects relating to the variability of returns in our sample, with the exception of emerging markets.Practical implicationsThe dispersion of returns can predict the swings of long-term volatilities of Nordic and Europe markets, while the risk aversion factor can predict the long-run volatilities of sustainable markets except Nordic.Originality/valueThe current study presents, for the first time in the sustainable finance literature, an empirical analysis of herding and risk aversion in sustainable investment returns over time. Our findings offer valuable insights to fund managers, finance professionals and investors, providing them with an opportunity to proactively manage their portfolios and reduce financial risk by understanding the volatility behavior of sustainable investing. Furthermore, it is crucial to explore and understand how the dispersion of returns and risk aversion interact with sustainable markets for the construction of optimal portfolios.
Service failure type, response strategy and observers’ perceived helpfulness: evidence from TripAdvisor
Authors: Zhang Mingli , Cai Shensheng , Qiao Tong 
Published: December 2024

Purpose
In social media context, service failures become public domain, making them visible to vast audiences of customers who are virtually present. Thus, this study aims to discuss the roles of service failure type and management response on observers’ perceived helpfulness.

Design/methodology/approach
The authors conducted econometric analyses on a dataset incorporating 107,984 reviews and 34,641 management responses to negative reviews.

Findings
The results reveal that, for process failures, specifying a form of action (initiatives for solving the problem) is associated with more perceived helpfulness of reviews than accounts (explanation) or acknowledgments (recognition and acceptance), while responding to an outcome failure by providing an account is associated with increased perceived helpfulness of reviews.

Practical implications
For process failures, managers should make every effort to convince observers through specific actions that similar failures are less likely to occur in the future. For outcome failures, managers should strive to provide clear and fast explanations of the failure causes to restore observers’ evaluation of the firm’s capabilities.

Originality/value
The authors’ work extends sparse insights on observers and sheds new light on the effect of service failure type and response strategy on observers’ actual behaviors. The interplay between service failure type and response strategy provides guidance on how to use management responses to influence potential customers.
4D printing with the assistance of electrical stimulation on polylactic acid coated with carbon nanotubes
Authors: Gholizadeh Ledari Reza , Zolfaghari Abbas 
Published: December 2024

Purpose
This paper aims to present an innovative approach to fabricating an electrically responsive shape memory polymer (SMP).

Design/methodology/approach
Polymers that change shape over time when a stimulus is applied are known as SMPs. It uses polylactic acid (PLA) as the base material and carbon nanotubes (CNTs) as conductive additives. Instead of blending CNT with PLA, they were coated on the surface of the samples. The coating consisted of a mixture of CNT/ polycaprolactone. The samples were made using fused deposition modeling, an additive manufacturing method and the shape memory properties of the samples were analyzed under various parameters, including infill angle, coating layers and applied voltage. The voltage generates the Joule heating effect and results in the recovery of SMP to the original shape.

Findings
The highest conductivity of samples belongs to three coating layers, whose conductivity is equal to 0.51 S/cm. Under different parameter settings, the highest recorded shape recovery ratio reached 71.47% at voltage 60, infill angle 0 / 90 and two coating layers. This value emphasizes the remarkable ability of the developed material to return to its original shape. Furthermore, the maximum shape recovery speed observed was 0.3593 degree/s, providing valuable information about shape recovery speed under optimal conditions.

Originality/value
This paper presents the surface coating method and the effects of process parameters for activating shape memory using electric current. Compared to previous techniques, this method offers higher speed and requires less material, making it suitable for use in various industries.
A dialogue on trust in ethics processes for creative practice research education
Authors: Couzens Andrew James , Johnson Amy , Cattoni Jan 
Published: December 2024

Purpose
Securing ethical approval can be a frustrating and opaque experience for some creative practice research students who may find the processes required of them not well suited to their specific inquiry. This can lead to an erosion of trust between students and their institutions. This paper aims to synthesize perspectives representing both creative practice research supervisors and Institutional Review Boards (IRBs) to develop recommendations for improving processes and supporting creative practice research training.

Design/methodology/approach
The authors used dialogical inquiry (Wells et al., 2021) to facilitate a dialogue between the authors, who represent different stakeholders in the ethical review process for creative arts research students. Focus topics for the dialogue were developed collaboratively based on existing literature.

Findings
Based on the dialogue, the authors make the following recommendations: frame ethics as part of project design alongside aims and methodology rather than as project logistics; structure ethics review processes to ensure ongoing engagement between research students and IRBs; and share exemplars and templates as part of research training. Additionally, the authors advocate for the need to create continuing opportunities for dialogue.

Originality/value
Previous work interrogating ethical review processes for creative practice research has adopted a clear position from either a creative practice researcher or an IRB member perspective. This dialogue facilitates a novel synthesis of these positions, ensuring the recommendations support the objectives of both IRBs and creative practice researchers.
Robust positioning in extreme environments: a tightly coupled GNSS-vision-inertial-wheel odometer framework
Authors: Zhang Yahui , Li Aimin , Li Haopeng , Chen Fei , Shen Ruiying 
Published: December 2024

Purpose
A tightly coupled global navigation satellite system (GNSS)-Vision-IMU-wheel odometer (GVIWO) system is proposed, which can realize robust positioning in extreme environments. The purpose of this study is to achieve adaptive initialization in complex environments, sensor anomaly detection and processing, and adaptive robust localization in extreme environments.

Design/methodology/approach
Adaptive initialization includes traditional dynamic and static initialization and extreme condition initialization. To deal with the unstable visual features in the state of excited motion, a method of wheel odometer assisted initialization is designed. According to the abnormal condition of the sensor, the anomaly detection and attenuation mechanism are designed to realize the accurate positioning of the sensor under abnormal condition.

Findings
Tight coupling optimization of GNSS signals, RGB+Depth Map cameras, inertial measurement units and wheel odometers ensures accurate positioning in both indoor and outdoor environments. Through open data sets and field validation experiments, the proposed tightly coupled system has strong adaptability, especially in extreme environments.

Originality/value
A new framework is proposed by integrating GNSS, visual, inertial measurement unit (IMU) and wheel odometer sensors to form an efficient positioning solution. An adaptive initialization method is proposed to enhance the robustness and real-time performance of the positioning system in complex and dynamic environments. A mechanism for detecting and attenuating sensor anomalies is designed, enabling quasideterministic positioning under sensor anomalies.
Unraveling the joint effect of firm and stakeholder pro-environmental engagement on firm economic rewards: an application to waste management services
Authors: Erbetta Fabrizio , Abrate Graziano 
Published: December 2024

Purpose
This paper aims to examine whether the pro-environmental engagement (PEE) of firms in response to the environmental expectations of stakeholders increases firm market power and whether the level of stakeholder PEE can counterbalance this effect.

Design/methodology/approach
This study draws on stakeholder theory and the co-production paradigm. The stakeholder perspective provides a theoretical basis for the increase in market power of organizations that improve their PEE, while co-production paradigm provides a theoretical foundation for the negative impact of stakeholder PEE on market power. The empirical evidence is derived from waste management services in Italy. The authors use a stochastic frontier approach to determine firm market power indices in relation to firm and stakeholder PEE.

Findings
The results confirm that market power increases when stakeholder expectations are met, while stakeholder commitment (mainly service recipients) challenges this effect. Furthermore, the findings suggest the existence of a self-reinforcing mechanism, as organizational efforts tend to keep pace with the empowerment of service recipients.

Originality/value
The originality of the study lies in the use of market power as a performance parameter, which has the advantage of being directly related to the acquisition of monopoly rents. In this context, the sustainability efforts of firms have strategic valence, as they allow them to approach a monopolistic condition, while the co-production efforts of service recipients can mitigate this socially undesirable outcome.