bluuebunny/crossref_metadata_2025_split huggingface dataset:
Antioxidative effects of haloarchaeal bacterioruberin in pangasius emulsion sausage during refrigerated storage
Authors: Reddy Ramakrishna , Xavier K. A. Martin , Balange Amjad K. , Kumar H. Sanath , Kumar Panda Satyen , Nayak Binaya Bhusan 
Published: February 2025

AbstractThe present study used haloarchaeal bacterioruberin (BR), a carotenoid pigment, to improve color and reduce fat oxidation. The BR is incorporated as 10 mg/kg (T1), 25 mg/kg (T2), and 50 mg/kg (T3) in the emulsion sausages, and 200 mg/kg butylated hydroxytoluene was used as a positive control (T4). Sausages without any antioxidant were taken as control (C). During refrigerated storage, pH, color, fat oxidation products, protein degradation products, and microbial counts were measured along with sensory analysis at 7‐day intervals. Control sausages were rejected by sensory panelists by the 28th day, whereas antioxidant‐treated groups could be stored till the 42nd day. Instrumental color revealed the highest redness (a*) in the T3 (2.03). During the storage Lower peroxide value, free fatty acid, and thiobarbituric acid‐reactive substances were found in BR‐added treatments. Present results suggest the inclusion of BR successfully improved the color and prevented the oxidation of lipids without affecting the gel strength of emulsion sausages.Practical ApplicationThis study demonstrated the potential use of bacterioruberin, a powerful antioxidant carotenoid, in emulsion sausages. This is the first report of using bacterioruberin for food preservation; it showed promising results by reducing fat oxidation and enhancing color in emulsion sausages. This opens up new possibilities for the food industry to use bacterioruberin as both an antioxidant to protect high‐fat foods from fat oxidation and as a food colorant.
A Short‐Read Amplicon Sequencing Protocol and Bioinformatic Pipeline for Ecological Surveillance of Dipteran Disease Vectors
Authors: Lima‐Cordón Raquel , Mohabir Jason Travis , Sooklall Mohini , Zurita Aina Martinez , Shieh Meg , Knox Cheyenne , Gobran Sabrina , Johnson Zachary , Laws Margaret , Panchal Ruchit , Niles‐Robin Reza , Cox Horace , Grillet Maria Eugenia , Moreno Jorge E. , Herrera Socrates , Quinones Martha , Early Angela M. , Tennessen Jacob A. , Neafsey Daniel E. 
Published: February 2025

ABSTRACTVector control remains an important strategy worldwide to prevent human infection with pathogens transmitted by arthropods. Vector control strategies rely on accurate identification of vector taxa along with vector‐specific biological indicators such as feeding ecology, infection prevalence and insecticide resistance. Multiple ‘DNA barcoding’ protocols have been published over the past several decades to support these applications, generally relying on informal manual approaches such as BLAST to assign taxonomic identity to the resulting sequences. We present a standardised informatic pipeline for analysis of DNA barcoding data from dipteran vectors, VecTreeID, that uses short‐read amplicon sequencing (AmpSeq) coupled with sequence similarity assessment (BLAST) and an evolutionary placement algorithm (EPA‐ng) to achieve vector taxonomic identification, capture bionomic features (blood and plant meal sources), determine Plasmodium infection status (for anopheline mosquitoes) and detect target‐site insecticide resistance mutations. The VecTreeID pipeline provides uncertainty in assignment through identifications at varying levels of taxonomic rank, a feature missing from many approaches to DNA barcoding, but important given gaps and labelling problems in public sequence databases. We validated an Illumina‐based implementation of VecTreeID on laboratory and field samples, and find that the blood meal amplicons can detect vertebrate DNA sequences up to 36 h post‐feeding, and that short‐read sequencing data are capable of sensitively detecting minor sequences in DNA mixtures representing multi‐species blood or nectar meals. This high‐throughput VecTreeID approach empowers researchers and public health professionals to survey and control arthropod disease vectors consistently and effectively.
Product Development with Lurking Patentees
Authors: Hovenkamp Erik , Lemus Jorge , Turner John L. 
Published: February 2025

ABSTRACTInnovative product developers are often uninformed about relevant patents. Patentees may exploit this by strategically “lurking”—refraining from ex ante licensing in the hope that developers will unwittingly infringe, creating an opportunity for “patent holdup.” We contribute to the literature by introducing a framework to explore when strategic lurking is profitable and how it affects developers' technology decisions. We find that lurking is pervasive in equilibrium, and it may lead to inefficient investment or adoption of inferior technologies. Commitments to license patents on reasonable (or “FRAND”) terms can improve efficiency and benefit all parties.
The Buyer Power Effect of Retail Mergers: An Empirical Model of Bargaining with Equilibrium of Fear
Authors: Bonnet Céline , Bouamra‐Mechemache Zohra , Molina Hugo 
Published: February 2025

ABSTRACTWe develop a bilateral oligopoly framework with manufacturer‐retailer bargaining to analyze the impact of retail mergers on market outcomes. We show that the surplus division between manufacturers and retailers depends on three bargaining forces and can be interpreted in terms of an “equilibrium of fear”. We estimate our framework in the French soft drink industry and find that retailers have greater bargaining power than manufacturers. Using counterfactual simulations, we highlight that retail mergers increase retailers' fear of disagreement relative to that of manufacturers, which weakens their buyer power and leads to higher wholesale and retail prices.
Tactical Emergency Medical Services in Copenhagen, Denmark: A mixed methods design study introducing a new concept
Authors: Glahn Jonas Erasmus Egede , Heltø Kim Minh Michael , Alstrøm Henrik , Petersen Marian Christin , Gärtner Rune 
Published: February 2025

AbstractAims and ObjectivesThe purpose of this study was to introduce the concept of Tactical Emergency Medical Services (TEMS) in Denmark and to explore the TEMS providers' and the Danish Police Forces' confidence in the TEMS concept in case of a terrorist attack within the Capital Region of Denmark.BackgroundTerrorist attacks pose a major challenge to prehospital emergency medical services when it comes to reaching trapped victims. The threat to healthcare professionals limits access and creates a so‐called therapeutic vacuum, leading to delayed treatment and evacuation of victims and ultimately increases mortality.MethodsThis study used a mixed methods triangulation approach, integrating qualitative data from an interview study and quantitative data from a register study. The interview study consisted of 18 semistructured interviews conducted across three interview groups (Copenhagen TEMS physicians/paramedics, senior on‐site commanders, and tactical and medical officers from the Copenhagen Police Department) with six participants in each group. Data from the interview study were analyzed using thematic framework analysis. The register study used data from the TEMS database, including 61 assignments from 2019 to 2020, that were analyzed using descriptive statistics.ResultsThe interview study found three core themes in each interview group. The main findings included insights on the advantages of having healthcare professionals in the warm zone, the prerequisites for the concept's success, TEMS's cooperation with the Danish Police Force, and challenges concerning healthcare providers inside tactical zones. The participants expected TEMS' presence inside the warm zone could improve triage and medical treatment, free police resources, increase police safety, optimize preparedness of the health sector, and significantly reduce the therapeutic vacuum. Data from the register study demonstrated that TEMS have had prior assignments in the warm and hot zones.ConclusionAll participating groups have high confidence in TEMS to close the gap of the therapeutic vacuum and deliver advanced physician and paramedic‐based tactical medicine inside the warm zone in case of a terrorist attack in Copenhagen, Denmark.Editorial CommentThis study includes both provider interviews and registry data to support insight into processes of Tactical Emergency Medicine. The authors discuss possible advantages of having emergency medical personnel in the major incident area early to support a coordinated response.
From Policy to Plate: The Impact of Livestock and Poultry Regulation on Meat Consumption in Rural China
Authors: Yan Zhen , Feng Hao , Li Ziwei , Lin Wen 
Published: February 2025

ABSTRACTGiven the negative externalities generated by pollution from livestock and poultry production, governments have implemented environmental regulations targeting the agricultural sector. This study uses China's livestock and poultry regulation (LPR) as a quasiexperiment and evaluates its impacts on rural residents’ meat consumption. Utilizing the data from China Health and Nutrition Surveys, our findings reveal an average reduction of 15.1 g in daily meat consumption per rural resident, primarily attributed to meat price increases. However, nonfarm employment would alleviate the negative impact of LPR on meat consumption. Residents with higher income, engaging in nonfarm jobs, and higher dietary knowledge exhibit lower mean intake reduction. Furthermore, for individuals with excessive meat intake, the regulation reduces their meat consumption, while exerting little effects on those with inadequate or moderate meat consumption. Our findings have important policy implications regarding the trade‐offs between food security and nutrition and environmental protection, in particular in rural areas.
Validating the MIMIC score as a predictor of successful spontaneous stone passage in patients managed conservatively for ureteric colic
Authors: Milton T. M. , Noll D. N. , Stapleton P. S. , Zhang P. Z. , Chieng J. C. , Chow J. C. , Hewitt J. H. , Stroman L. S. , Van Beek S. V. , Steele R. S. 
Published: February 2025

AbstractBackgroundsThe MIMIC score was designed to predict the likelihood of spontaneous stone passage (SSP) in patients presenting with ureteric colic and given a trial of conservative management. The objective of this study was to determine the external validity of the MIMIC score.MethodsCross‐sectional, retrospective cohort study that reviewed patients over a 2‐year period presenting with ureteric colic who were given a trial of conservative management. Patient‐factors were recorded, and their MIMIC score calculated and compared to the observed outcome. Discrimination was determined using an area under the receiver‐operating curve with an AUC >0.7 considered validated and calibration using calibration plot.ResultsThree hundred ninety‐nine patients were included. The median age was 49 (IQR 37–60). 79% were males. The mean stone size for successful SSP was 3.8 mm compared to 4.8 mm for failed SSP. 88% of stones passed spontaneously. The AUC was 0.68 (95% CI: 0.60–0.77). The calibration plot showed an underestimation of SSP. In the lowest quintile of patients with a mean predicted SSP rate of 46%, the observed SSP rate was 74% (95% CI: 63–83%). With a higher predicted SSP rate, the MIMIC score was accurate. In the highest quintile of patients with a mean predicted SSP rate of 90%, the observed SSP rate was 92% (95% CI: 84–97%).ConclusionIn this population, the prediction of the MIMIC score were not sufficiently accurate. There was overall an underestimation of SSP. The MIMIC score was accurate for patients who had a higher likelihood of SSP (>83%).
Outcomes associated with fetal nuchal translucency between 3.0 and 3.4 mm in the first trimester
Authors: Vriendt Manon D. E. , Rooryck Caroline , Madar Hugo , Coatleven Frédéric , Vincienne Marie , Prier Perrine , Naudion Sophie , Sentilhes Loïc , Bouchghoul Hanane 
Published: February 2025

AbstractIntroductionDecisions concerning nuchal translucency (NT) between 3.0 and 3.4 mm remain controversial, particularly regarding whether to first calculate the combined first trimester screening test or to proceed directly with invasive testing. The literature suggests an increased risk of chromosomal aberration, as well as pathogenic copy number variations (CNVs) on chromosomal microarray, for fetuses with NT between 3.0 and 3.4 mm. The aim of this study was to describe genetic findings of fetuses with NT between 3.0 and 3.4 mm in the first trimester. The secondary objective was to describe ultrasound findings and adverse outcomes for these fetuses. The third objective was to compare genetic, ultrasound findings and adverse outcomes of fetuses with NT between 3.0 and 3.4 mm to those with NT ≥3.5 mm.Material and MethodsWe conducted an observational, retrospective study in a referral center between 2017 and 2022. Genetic and ultrasound findings were compared between fetuses with NT between 3.0 and 3.4 mm and those with NT≥3.5 mm. An adverse outcome was defined as one of the following: miscarriage, perinatal death (stillbirth or neonatal death) or termination of pregnancy at parental request, and all major abnormalities or genetic disorders diagnosed before or after delivery.ResultsWe included 404 fetuses with NT≥3.0 mm who had invasive testing with available karyotype and chromosomal microarray, among whom 20.8% (84/404) had NT between 3.0 and 3.4 mm. The rate of adverse outcomes among fetuses with NT between 3.0 and 3.4 mm was 32.1% (27/84). The rates of chromosomal aberration, pathogenic CNVs, and major ultrasound abnormalities were 16.7% (14/84), 6.0%(5/84), and 9.2% (6/65), respectively, for fetuses with NT between 3.0 and 3.4 mm. In comparison, fetuses with NT greater than 3.5 mm had higher rates of chromosomal aberration and major ultrasound abnormalities, with rates of 47.5% (152/320) and 30.2% (49/162) respectively compared to 16.7% (14/84) and 9.2% (6/65) for fetuses with NT between 3.0 and 3.4 mm (p < 0.001 for both comparisons). However, the rate of pathogenic CNVs was not significantly different between the two groups, with rates of 1.9% (6/320) for NT≥3.5 mm and 6.0% (5/84) for NT between 3.0 and 3.4 mm (p = 0.06).ConclusionsThe rate of chromosomal aberration and pathogenic CNVs on chromosomal microarray is high among fetuses with NT between 3.0 and 3.4 mm, although these rates remain lower than those observed among fetuses with NT≥3.5 mm.
Urban–rural disparities in the prevalence and trends of loneliness among Chinese older adults and their associated factors: Evidence from machine learning analysis
Authors: Zhu Boyu , Wu Lin 
Published: February 2025

AbstractIn the context of rapid aging development, exploring the predictive factors of older adults' loneliness and its urban–rural differences is of great significance for promoting the psychological health of older adults. This study selected 30 variables from the three waves of China Family Panel Studies (CFPS) data in 2016, 2018, and 2020, using a random forest classifier to explore the predictive factors of loneliness. The sense of loneliness among rural older adults is significantly higher than that of urban older adults. Among the top 10 predictors of loneliness, there are seven common factors in urban and rural, including sleep quality, marital status, confidence in the future, weekly family dinners, life satisfaction, physical discomfort in the past 2 weeks, and relationship with children. The other three different predictive factors for urban older adults are weekly movie and TV duration, family size, and family net worth, while self‐rated health, health change, and per capita family income set the rural older adults apart. In addition, the urban–rural differences in the predictive factors of older adults' loneliness show different development trends in the time dimension. Paying attention to the predictive factors that contribute to the high ranking of older adults' loneliness and the widening trend of urban–rural differences is highly required.
‘The city is not for us’: Ethics, everyday sexism, and negotiating unwanted encounters during fieldwork
Authors: Rose Morag 
Published: February 2025

AbstractThis paper is an autoethnographic account of situated ethics and risk during fieldwork. Specifically, it discusses experiences of gendered street harassment while conducting walking interviews with women in Manchester, UK. These are shared as a series of vignettes, each illustrating a different incident. It utilises Vera‐Gray's (2016) conception of ‘men's stranger intrusions’ to understand what happened because each encounter irrevocably changed the trajectory of the research conversations it disrupted. Navigating through these incidents, this work reconsiders the ethics of empirical research in a UK city and what constitutes an acceptable risk. It discusses the impact of everyday sexism and street harassment on both the researcher and their work. Making these stories visible matters, because it centres the restrictions women may feel and strategies they develop to enable their continued participation in public life despite gendered threats. Thinking through possible implications for future research, it concludes with some practical recommendations to mitigate potential harms. Mitigations do not negate the need for wider structural changes to eliminate the threat of gender‐based violence, nor do they signal resignation that we must minimise ourselves, or that perpetrators must not take responsibility for violence against women. These suggestions for practical actions researchers can take are based on lived experience and shared in a spirit of care, resistance, solidarity, and rage.
Clinical and molecular characteristics of paediatric mature B‐cell acute lymphocytic leukaemia and non‐Hodgkin lymphoma with bone marrow involvement: A joint study between the CCCG leukaemia and lymphoma groups
Authors: Zhao Jie , Liu Tian‐Feng , Wu Ke‐fei , Yang Liang‐Chun , Xu Xue‐Ju , Lu Jun , Shao Jing‐Bo , Li Fu , Ma Fu‐Tian , Guo Xia , Li Hui , Liu Ai‐Guo , Wang Ning‐Ling , Shen He‐Ping , Li Yang , Liu Si‐Xi , Liang Chang‐Da , Shen Shu‐Hong , Fang Yong‐Jun , Gao Yi‐Jin 
Published: February 2025

SummaryMature B‐cell acute lymphocytic leukaemia (B‐ALL) is distinguished from B‐cell non‐Hodgkin lymphoma (B‐NHL) by the arbitrariness of the 25% cut‐off, and given that the percentage of bone marrow (BM) blasts can vary according to site of aspirate, we refrained from differentiating mature B‐ALL from B‐NHL with BM infiltration. A total of 156 patients from the Chinese Children Cancer Group with BM blasts of more than 5% and consistent with immunophenotypic features of mature B cells were included in this study. The 2‐year progression‐free survival, 2‐year event‐free survival and 2‐year overall survival were 76.6 ± 3.6%, 69.7 ± 3.7% and 80.1 ± 3.3% respectively. Central nervous system (CNS) involvement, serum ferritin levels higher than four times normal and rituximab no more than two doses were associated with lower PFS. Male, bulky disease and head/neck region involvement were associated with higher rate of CNS invasion. We performed an integrative transcriptomic characterization of 36 cases. Structure variant included IG::MYC, IGH::CACS11, MEF2D::BCL9, IGH::VPS53 and ACIN1::NUTM1. SNV analysis uncovered driver variations affecting 10 recurrently mutated genes including ID3, TP53, MYC, ARID1A, SMARCA4, DDX3X, CCND3, RHOA, SMARCB1, FOXO1 and GNA13. Mature B‐ALL/B‐NHL with BM involvement was a heterogeneous group of malignancies in both clinical features and genetic alternations. Genetics analysis was helpful for making accurate diagnoses and guiding appropriate therapeutic strategies.
Interactions between ADGRF1 (GPR110) and extracellular matrix proteins govern its effects on tumorigenesis in HER2‐positive breast cancer
Authors: Abdulkareem Noor Mazin , Bhat Raksha , Castillo Micah , Jung Sung Yun , Vasaikar Suhas , Nanda Sarmistha , Ruiz Alexis , Shea Martin , Cao Wangjia , Veeraraghavan Jamunarani , Kim Hee‐Yong , Bawa‐Khalfe Tasneem , Hussain Tahir , Liu Xinli , Gunaratne Preethi , Schiff Rachel , Trivedi Meghana V. 
Published: February 2025

Background and PurposeWe and others have previously shown that ADGRF1, an adhesion G protein‐coupled receptor, is overexpressed and associated with poor survival in many cancers, including human epidermal growth factor receptor‐2 (HER2) breast cancer (BC). Also, we have reported the tumour‐promoting function of ADGRF1 using preclinical models of HER2+ BC. In this study, we investigated the effect of ADGRF1 overexpression in an orthotopic in vivo model as well as downstream signalling of ADGRF1 in HER2+ BC.Experimental ApproachWe utilized a doxycycline (Dox)‐induced ADGRF1 overexpression system in HER2+ BC cell lines and performed various in vitro and in vivo studies. Following ADGRF1 overexpression in the presence/absence of Matrigel, laminin‐111 or collagen‐IV, we performed the mammosphere assay to assess the tumorigenicity of breast epithelial cells, as well as cAMP/IP1 assays and RNA‐sequencing, to understand the receptor function and pharmacology. We conducted cross‐linking‐aided immunoprecipitation and mass spectrometry to confirm the physical interaction between ADGRF1 and the extracellular matrix proteins present in Matrigel.Key ResultsWe found that ADGRF1 switched from a tumour‐promoting to tumour‐suppressive function upon interaction with laminin‐111. Interaction of ADGRF1 with laminin‐111 resulted in inhibition of Gαs coupling and STAT3 phosphorylation, induction of senescence, increase in HER2 expression, and improvement of sensitivity to anti‐HER2 drugs in HER2+ BC.ConclusionsADGRF1 switches from a tumour‐promoting to tumour‐suppressive function upon interaction with laminin‐111, leading to improvements in sensitivity to anti‐HER2 drugs. Leveraging ADGRF1 interactions with laminin‐111 may allow the design of novel therapies against ADGRF1 in HER2+ BC.
NEK2 Control of Esophageal Squamous Cell Carcinoma Growth Based on Circadian Oscillation
Authors: Zeng Boning , Sun Chao , Li Nan , Chen Qiuling , Rao Manni , Li Kai , Liu Xiaodi , Xie Shouxia , Cheng Jiwu , Wang Shaoxiang , Wang Xiao 
Published: February 2025

ABSTRACTEsophageal squamous cell carcinoma (ESCC) is a globally prevalent malignancy known for its aggressive nature and unfavorable outcomes. Identifying new biomarkers is crucial for the early detection and improved prognostication of ESCC. The circadian clock and NIMA‐related kinase 2 (NEK2) are pivotal in cancer development. While the impact of circadian rhythm disruptions on ESCC progression is evident, the specific contribution of NEK2 to these changes is not well understood. Our study discovered NEK2 as a consistently differentially expressed gene across multiple datasets, with elevated expression in ESCC tissues. Notably, NEK2 overexpression was linked to increased ESCC cell proliferation, whereas its inhibition led to reduced cell growth and proliferation. Pathway analyses, including KEGG and Gene Set Enrichment Analysis (GSEA), indicated NEK2's association with established pathways like the cell cycle, and intriguingly, identified the circadian rhythm as a novel pathway influenced by NEK2. RNA sequencing data demonstrated NEK2's circadian rhythmic expression, and subsequent in vitro experiments confirmed its oscillation in synchronized ESCC cells. Moreover, we found a positive correlation between the efficacy of the NEK2 inhibitor INH6 and NEK2 expression levels in ESCC. In conclusion, our findings position NEK2 as a time‐dependent oncogene and a potential biomarker in ESCC, highlighting its role in both tumorigenesis and the circadian rhythm.