bluuebunny/crossref_metadata_2025_split huggingface dataset:
Differential Metabolism-associated Gene Expression of Duck Pancreatic Cells in Response to Two Subtypes of Duck Hepatitis A Virus Type 1
Authors: Chen Zhen , Shi Shao-hua , Huang Yu , Huang Cui-qin , Liu Rong-chang , Cheng Long-fei , Fu Guang-hua , Chen Hong-mei , Wan Chun-he , Fu Qiu-ling 
Published: February 2021

Abstract
Duck hepatitis A virus type 1 (DHAV-1) causes a highly contagious disease in domestic ducklings, which is traditionally characterized by lesions in the liver and rarely in the pancreas. However, several outbreaks of DHAV-1, which were characterized by yellow coloration and hemorrhage in pancreatic tissues, have occurred in China. The causative agent was named as pancreatitis-type DHAV-1. Genomic sequencing indicated variation rates of 3.4-6.5% in the genome of the pancreatitis-type DHAV-1 compared with those of the classical type DHAV-1. The antigenic relationship between the pancreatitis-type DHAV-1 and classical type DHAV-1 indicated large variation. However, the mechanism involved in infection of the pancreatitis-type DHAV-1 is still unclear. Transcriptome analysis of duck pancreas infected with classical type DHAV-1 and pancreatitis-type DHAV-1 was carried out. Following deep sequencing with Illumina-Solexa, a total of 53.9 Gb clean data were obtained from the cDNA library of the pancreas and a total of 29,597 unigenes with an average length of 993.43 bp were generated following de novo sequence assembly. The expression levels of D-3-phosphoglyceratedehydrogenase, phosphoserine aminotransferase, phosphoserine phosphatase, which are involved in glycine, serine and threonine metabolism pathway, were significantly downregulated in the pancreatitis-type DHAV-1-infected group compared with those of the classical type DHAV-1-infected group. These findings provide information regarding differential expression levels of metabolism-associated genes between pancreatitis-type DHAV-1 and classical type DHAV-1, indicating that intensive metabolism disorders may contribute to different subtypes of DHAV-1-infection.
Association of subclinical atherosclerosis with retinal vein occlusion: A prospective, case-control study
Authors: Lyu Minhyung , Lee Yonggu , Kim Byung Sik , Kim Hyun-Jin , Hong Rimkyung , Shin Yong Un , Cho Heeyoon , Shin Jeong-Hun 
Published: February 2021

Abstract
Retinal vein occlusion (RVO) is known to be associated with atherosclerotic cardiovascular risk factors, but the association between specific markers of subclinical atherosclerosis has not been established. To investigate this association, we compared the results of cardiovascular examinations of 76 patients with RVO and 175 age- and sex-matched control subjects. Low-density lipoprotein cholesterol (LDL-C) level and brachial-ankle pulse wave velocity (baPWV) were significantly higher in the RVO group than in the control group. Carotid plaque (54.3% vs. 28.6%, p = 0.004) was more frequent in the RVO group. Multivariate logistic regression analysis showed that the presence of carotid plaque [odds ratio (OR) 3.15, 95% confidence interval (CI) 1.38–7.16, p = 0.006], as well as smoking, LDL-C level, and baPWV was associated with RVO. Additionally, a multinomial logistic regression model showed that the presence of carotid plaque (OR 3.94, 95% CI 1.65–9.41, p = 0.002) and LDL-C level were associated with branch RVO, whereas smoking and baPWV were associated with central RVO. In conclusion, RVO is associated with subclinical atherosclerosis markers, including carotid plaques and baPWV. These results support the hypothesis of a vascular etiology of RVO and suggest the evaluation of subclinical atherosclerosis in patients with RVO.
Mesenchymal Stem Cell-Derived Exosomes Protect Trabecular Meshwork from Oxidative Stress
Authors: Li Ying-chao , Zheng Juan , Wang Xi-zi , Wang Xin , Liu Wen-jing , Gao Jian-lu 
Published: February 2021

Abstract
This study aims to investigate potential effects of exosomes derived from bone marrow mesenchymal stem cells (BMSCs) on trabecular meshwork cells under oxidative stress and predict candidate genes associated with this process. Trabecular meshwork cells were pretreated with BMSC-derived exosomes, and exposed to 0.1mM H2O2. Survival rate of trabecular meshwork cells was measured with CCK-8 assay. Production of iROS was measured using a flow cytometer. RT-PCR and ELISA were used to detect mRNA and protein levels of inflammatory cytokines and matrix metalloproteinases (MMPs). RNA sequencing for trabecular meshwork cells from Exo- and control groups was performed on BGISEQ500 + bgiseq-500 platform. Phenotypically, pretreatment of BMSC-derived exosomes improves survival rate of trabecular meshwork cells exposed to H2O2, reduces production of iROS, and inhibits expression of inflammatory cytokines, whereas increases expression of MMPs. There are 23 DEmiRNAs, 307 DElncRNAs, and 367 DEmRNAs differentially expressed between Exo- and control groups. Exosomes derived from BMSCs may protect trabecular meshwork cells from oxidative stress. Candidate genes responsible for beneficial effects ,such as DIO2 and HMOX1, such as DIO2 and HMOX1, are predicted.
A Randomized Crossover Air Purifier Intervention Study of Patients with Stable Coronary Artery Disease
Authors: Liu Zhe , Wang Qin , Li Na , Xu Chunyu , Li Yunpu , Zhou Jun , Liu Liu , Zhang Haijing , Mo Yang , Xu Dongqun 
Published: February 2021

Abstract
Exposure to PM2.5 will accelerate the progression of cardiovascular diseases. Air purifier can reduce the PM2.5 exposure and theoretically alleviate the influence of PM2.5 on patients with stable coronary artery disease (SCAD). However, few studies of the protective effect showed significant results because the interferent effects of routine medication had not been taken into account. In order to explore the actual effect on patients with SCAD, we conducted a randomized crossover air purifier intervention trial. Levels of the subjects’ PM2.5 exposure during intervention and cardiovascular indicators (inflammation, coagulation, plaque stability, and blood lipids) after intervention were detected, meanwhile the information of drug use was obtained by questionnaire. Then the kinds of drug used by more than 20% of the subjects were sorted out. And the influence of these drugs on cardiovascular indicators was summarized through literature review. Based on that, the drug use was included as a variable in linear mixed effects models that used to analyze the associations between PM2.5 exposure reduction by air purifier and cardiovascular indicators. The result revealed that the interpretation contribution rate of drug use was more than that of PM2.5 exposure. The level of C-reactive protein (CRP) significantly decreased on lag1, lag01 and lag02, while the level of high-density lipoprotein cholesterol (HDL-C) significantly increased on lag0, lag1 and lag01 associated with an interquartile range (IQR) decrease in PM2.5 exposure. The study can provide reference for future relevant research in methodology.
Predictors and Prognostic Significance of Persistent Fluid Overload: a Longitudinal Study in Chinese Peritoneal Dialysis Patients
Authors: Ng Jack Kit-Chung , Kwan Bonnie Ching-Ha , Chan Gordon Chun-Kau , Chow Kai-Ming , Pang Wing-Fai , Cheng Phyllis Mei-Shan , Leung Chi-Bon , Li Philip Kam-Tao , Szeto Cheuk-Chun 
Published: February 2021

Abstract
Background: Cross-sectional studies showed that fluid overload (FO) measured by bioimpedance spectroscopy (BIS) predicted adverse outcomes in patients on peritoneal dialysis (PD). We describe the longitudinal change in volume status in Chinese PD patients, and determine its relation with clinical outcomes.Methods: We performed a single-center, retrospective analysis of all PD patients who underwent repeated BIS from 2010 to 2015. FO was defined by relative hydration index (RHI; volume of overhydration adjusted by extracellular water >7%). Variability of volume status (VVS) was denoted by the standard deviation of all RHI. The association of time-averaged RHI and VVS on patient and technique survival was explored by a competing risk model.Results: A total of 269 patients were followed for a median of 47.1 months. Multivariate mixed linear regression revealed that RHI was significantly associated with time-varying systolic blood pressure, and inversely with time-varying albumin level, lean tissue index and fat tissue index (P <0.0001 for all). Patients without FO at baseline, as compared with those who had FO, showed significantly more fluid accumulation with time (adjusted between-group mean difference in RHI: 3.2% per year, 95% confidence interval [CI] 1.5 to 4.9%, P =0.0002). Time-averaged RHI independently predicted patient survival (subdistribution hazard ratio [SHR] 1.05, 95% CI 1.03-1.08, P <0.0001) and technique survival (SHR 1.04, 95% CI 1.01-1.06, P =0.001), whereas VVS did not.Conclusions: Persistent FO was a strong predictor of patient and technique failure. Repeated bioimpedance measurements for the monitoring of volume status provided additional prognostic information in PD patients.
Quantification of bone marrow interstitial pH and calcium concentration by intravital ratiometric imaging
Authors: Yeh Shu-Chi , Hou Jue , Wu Juwell , Camargo Fernando , Lin Charles 
Published: February 2021

Abstract
The fate of hematopoietic stem cells (HSCs) in the bone marrow can be directed by microenvironmental factors including extracellular calcium ion concentration (T h can vary significantly with bone remodeling, but the local e around individual HSCs in vivo remains unknown. Here we developed an intravital ratiometric imaging approach to quantify the absolute pH and in the mouse calvarial bone marrow, taking into account the pH sensitivity of the calcium probe and the wavelength-dependent optical loss through bone to ensure unbiased ratiometric analyses. We uncovered substantial heterogeneity in f (1.0 - 3.6 mM) surrounding steady-state HSCs. While the lowest a (0.3 mM) was found in regions dominated by bone formation, HSCs were not found in those locations. This work thus established a tool to further investigate t and pH in the HSC niche under malignant or stressed conditions and can be broadly applied to other tissue types.
Modified Superior Oblique Intrasheath Tenectomy in a-pattern With Superior Oblique Overaction&nbsp;&nbsp;
Authors: Sun Chunhua , Wang Ze , Xia Bo 
Published: February 2021

Abstract
Background: To evaluate the effect of modified superior oblique intrasheath tenectomy (MSOIT) on superior oblique overaction (SOOA) with A-pattern.Methods: We retrospectively reviewed the data of 66 patients (130 eyes) with SOOA and A-pattern underwent MSOIT at the nasal border of the superior rectus under an operating microscope between January 1, 2004 and December 31, 2018.. The preoperative and postoperative SOOA, objective torsion, ocular motility, and A-pattern deviation findings were compared. The correlation between the preoperative A-pattern deviation and the corrected deviation was analysed. All patients were followed up for more than 12 months. Results: The superior oblique (SO) tendon fibres were resected, and the sheath was preserved in all patients. The average follow-up period was 33.45±29.88 (range: 12–122) months. The mean SOOA deviation improved from 2.95±0.54 to 0.34±0.55 (P < 0.001), while the A-pattern deviation difference between upgaze and downgaze improved from 23.15±7.64 prism dioptres (PD) to 3.50±2.90 PD (P < 0.001). The average objective fundus intorsion value improved from +2.96±0.58 to +0.38±0.60 (P < 0.001). The magnitude of correction in A-pattern was significantly correlated with the preoperative severity of A-pattern (r = 0.812, P<0.001).Conclusions: MSOIT at the nasal border of the superior rectus (SR) under an operating microscope is safe and yields beneficial outcomes in patients with SOOA and A-pattern.
An approach for a sustainable decision-making in product portfolio design of dairy supply chain in terms of environmental, economic and social criteria
Authors: Kirilova Elisaveta Georgieva , Vaklieva-Bancheva Natasha , Vladova Rayka , Petrova Tatyana , Ivanov Boyan , Nikolova Desislava , Dzhelil Yunzile 
Published: February 2021

Abstract
The production of dairy products is related to water and energy costs and generation of large amounts of greenhouse gas (GHG) emissions and wastewater. This has a direct bearing on the reduction of natural resources, the negative impact on the environment as well as the rise of social concerns due to increased product costs, pollution, etc. Full sustainability of this type of systems can be achieved by optimizing all activities across the chain of raw materials, through production to customers while considering all aspects of sustainability - environmental, economic and social. The study proposes a multi-objective modelling approach for optimal design of three-echelon supply chain (SC) for production of dairy products according to different recipes where all aspects of sustainability are taken into consideration. The approach was implemented on a real case study from Bulgaria. Four mix integer nonlinear programming (MINLP) optimization models were defined without and with social impact consideration. They are solved using General Algebriac Modeling System (GAMS) software. The obtained results have shown how the social impact assessment affects the obtained optimal products portfolios and profits. In these cases, varying the values of environmental constraints also affect the products portfolios and profits without affect the social costs. The stricter constraints on the environment impact leads to higher economic costs and lower profits, and conversely, high values of environmental constraints result in higher profits and lower economic costs. The obtained solutions can be used in the decision-making process to achieve social sustainability of the considered dairy supply chain.
5-Aminosalicylic acid Attenuates Paraquat-induced Lung Fibroblasts Activation and Pulmonary Fibrosis of Rats
Authors: Chen Hui , Cui Jinfeng , Wang Juan , Wang Yuan , Tong Fei , Tian Yingping , Gong Yu , Ma Yu , Liu Liang , Zhang Xianghong 
Published: February 2021

Abstract
Pulmonary fibrosis is one of the most common complications of paraquat (PQ) poisoning, which becomes the focus of treatment. More and more studies have found that 5-Aminosalicylic acid (5-ASA) may be a prospective therapy against fibrotic diseases. In the present study, we observed whether 5-ASA could attenuate the pulmonary fibrosis in PQ-treated rats and human lung fibroblasts (WI38VA13) cells, and subsequently explored the possible underlying mechanisms. Wistar rats were divided into control group, 5-ASA group, PQ group and PQ + 5-ASA group. Rats were sacrificed on 3, 7, 14, and 28 days after PQ treatment. We observed pulmonary histopathological changes and fibrosis formation among different groups through hematoxylin and eosin (H&E) and Masson staining and TGF-β1, p-Smad3 and the peroxisome proliferator activated receptor γ (PPARγ) pulmonary content via immunohistochemical staining and Western blot. In addition, human lung fibroblasts WI38VA13 were also divided into control group, PQ group, 5-ASA group and PQ + 5-ASA group. And the role of TGF-β1 signaling pathway regulated factors (TGF-β1, p-Smad3 and PPARγ) were explored. Treatment with 5-ASA significantly inhibited the PQ-induced activation of TGF-β1 signaling pathway in human lung fibroblasts WI38VA13 cells. In conclusion, the results of this study suggested that 5-ASA has potential value in the treatment of PQ-induced pulmonary fibrosis via suppressing the activation of TGF-β1 signaling pathway.
Higher risk of chronic kidney disease and progressive kidney function impairment in primary aldosteronism than in essential hypertension. Case-control study
Authors: Argüeso María Fernández , Pascual-Corrales Eider , Rojano Nuria Bengoa , Cano Ana García , Mendiguchía Lucía Jiménez , Araujo-Castro Marta 
Published: February 2021

Abstract
Purpose: To study the differences in the prevalence, risk, and grade of control of different cardiometabolic comorbidities in patients with primary aldosteronism (PA) and essential hypertension (EH) matched by age, sex, and blood pressure levels at diagnosis.Methods: Case-control study of a secondary base (PA patients in follow-up in a tertiary hospital between 2018-20). Controls were patients with EH, matched by age, sex, and baseline diastolic (DBP) and systolic blood pressure (SBP). Results: Fifty patients with PA and 50 controls were enrolled in the study. At diagnosis, PA patients had a higher prevalence of chronic kidney disease (CKD) than controls (18.4% vs. 2.1%, P=0.008). No differences were detected in the prevalence of other cardiometabolic comorbidities nor in their degree of control (P>0.05). All patients received antihypertensive medical treatment and 10 PA patients underwent unilateral laparoscopic adrenalectomy.After a median follow-up of 31.9 [IQR=1.0-254.8] months, PA patients presented a greater degree of declination of kidney function than controls (Average decrease in glomerular filtration rate (MDRD-4) -17.6±3.1 vs -2.8±1.8 ml/min/1.73m2, P<0.001). There were no differences in the grade of SBP (P=0.840) and DBP control (P=0.191), nor in the risk of developing other comorbidities or in their degree of control.Conclusions: PA patients have a prevalence of CKD ten times higher than those with EH matched by age, sex, and blood pressure levels. Furthermore, the risk of kidney function impairment throughout the follow-up is significantly greater in PA patients and is independent of the degree of blood pressure control.
Modifying the Electronic and Optical Properties of Nano ZnS&nbsp;via Doping With Mn and Fe
Authors: Heiba Zein , Mohamed Mohamed , Shimy H. El , Badawi Ali 
Published: February 2021

Abstract
5% Fe and Mn-doped nano ZnS (Zn0.95A0.05S, A=Fe or Mn) was prepared using the thermolysis technique. The effect of Fe and Mn doping on the lattice parameter, crystallite size, and lattice microstrain was examined applying Rietveld refinement. The analysis showed the incorporation of Fe and Mn substitutionally for Zn ions; a result confirmed by Fourier transforms infrared spectroscopy spectra from the shift in the vibration bands upon doping. The energy of ZnS band gap was reduced by doping, giving ZnS new applications. The different defects inside the different samples were investigated using photoluminescence spectroscopy. The effect of Fe/ Mn doping and incorporation of atmospheric oxygen on the bandgap characteristics, the absorption, optical conductivity, refractive index and the extinction coefficient, reflectance, the real and imaginary parts of the dielectric constant were explored using density function theory calculation (DFT).
Stepwise Implementation of a Cardiovascular Risk Management Care Program in Primary Care: A Research Article
Authors: Smits Geert , Hollander Monika , Doorn Sander van , Bots Michiel 
Published: February 2021

Abstract
Background: Primary care plays a pivotal role in sustainable cardiovascular risk management (CVRM) but little is known about the organizational process of implementing the guidelines. The aim of the study was to describe the approach taken by a primary care group to implement the CVRM guideline.Methods: Stepwise introduction and implementation of a programmatic CVRM care program was organized and facilitated by the care group between April 2010 and April 2012 in 143 affiliated general practices with 190 committed general practitioners (GPs), in the vicinity of Eindhoven, Netherlands. Care group support comprised sufficient staff, support with data extraction based on ICPC and ATC codes and with identification of eligible patients by scrutinizing patient health records and adequate coding of disease conditions. Results: Within 2 years, patient selection based on availability of structured information on ICPC codes and risk factor levels from the electronic health records, led to 38.082 eligible patients in 2013. October 2020, the CVRM program is still running in 157 practices with 49380 patients receiving programmatic CVRM care. Linking problems between 8 different electronic health record systems and the multidisciplinary information system for integrated care ( Care2You) delayed adequate data collection until the beginning of 2013.Conclusion: Introduction and implementation of integrated primary care for cardiovascular risk management is feasible when a number of conditions are met: commitment of affiliated GPs, adequate coding of diagnoses and risk factors, a structured approach and additional funding for sufficient staff support. This approach constitutes the basis for long-term follow up and annual evaluation.
Neonatal Hypoxic-Ischemic Encephalopathy Diagnosis and Treatment: A National Survey in China
Authors: Wang Zheng , Zhang Peng , Zhou Wenhao , Xia Shiwen , Zhou Wei , Zhou Xiaoyu , Cheng Xiuyong , Shi Yuan , Lin Zhenlang , Song Dongli , Cheng Guoqiang 
Published: February 2021

Abstract
Background: Neonatal hypoxic-ischemic encephalopathy (HIE) affects as many as 100,000 infants each year in China. Therapeutic hypothermia reduces HIE related mortality and long-term neurodevelopmental disabilities. National guidelines for HIE management were published a decade ago. This study aimed to investigate the current status of HIE diagnosis and treatment in China. Method: This prospective cross-sectional national survey used a questionnaire evaluating practices related to HIE management. Descriptive statistics and Chi-square or Fisher's exact test were used, and a p-value of <0.05 was considered significant. Results: The 273 hospitals that completed the survey were located in 31 of the 34 provincial districts in China. Eighty-eight percent of the hospitals were Level III hospitals, and 74% treated ten or more HIE cases annually. Awareness rates of the national guidelines for HIE diagnosis, HIE treatment, and therapeutic hypothermia protocol were 85%, 63%, and 78%, respectively. Neurological manifestations and blood gas were used as HIE diagnostic criteria by 96% (263/273) and 68% (186/273) of the hospitals, respectively. Therapeutic hypothermia was used in 54% (147/273) of hospitals. The percentage of general hospitals that implemented therapeutic hypothermia (43%, 71/165) was significantly lower than that in maternity and infant hospitals (67%, 49/73) (χ2=11.752, p=0.001) and children’s hospitals (77%, 27/35) (χ2=13.446, p<0.001). Reasons for not providing therapeutic hypothermia included reduction of HIE cases in recent years (39%), high cost of cooling devices and treatment (31%), lack of training (26%), and safety concerns (4%). Among the hospitals that provided therapeutic hypothermia, 27% (39/147) were in full compliance with the recommended protocol. Eighty-one percent (222/273) of the hospitals treated HIE infants with putative neuroprotective agents alone or in combination with cooling. Ninety-one percent of the hospitals had long-term neurodevelopmental follow-up programs for infants with HIE. Conclusions: There is significant heterogeneity in HIE diagnosis and treatment in China. Therapeutic hypothermia has not become a standard of care for neonatal HIE nationwide. Unproven agents are widely used for HIE treatment. Nationwide standardization of HIE management and dissemination of therapeutic hypothermia represent the opportunities to reduce mortality and improve long-term neurodevelopmental outcomes of children affected by HIE.