bluuebunny/crossref_metadata_2025_split huggingface dataset:
208 MicroRNA Expression in Longissimus Muscle Biopsies During Feedlot Finishing of Angus Steers
Authors: Thomas Alexandra R , Justice S Maggie M , Duckett Susan K 
Published: October 2021

Abstract
Intramuscular fat or marbling (IMF) is highly desired in beef products due to its ability to improve carcass value. Studies indicate that finishing cattle on high concentrate diets increase marbling deposition over time-on-feed (TOF). Angus-cross steers (n = 7; 433 + 17 kg) were individually fed a high concentrate diet for a period of 124 d. Steers were weighed and ultrasounded every 30 d on feed. Longissimus muscle biopsies were taken between the 11th and 13th rib on alternating sides at d 0, 90, and 124. RNA was extracted from the biopsy samples and subjected to microRNA (miRNA) sequencing and qPCR for mRNA expression of key lipogenic genes. Data were analyzed with time-on-feed in the model. ADG was increased between d30 and d90 (P &lt; 0.05). The feed conversion ratio was lowest during d0 to d60 and increased from d90 to d124 (P &lt; 0.05). Fat thickness increased (P &lt; 0.05) between 90 to 124 d. Ultrasound intramuscular fat percentage increased (P &lt; 0.05) between 60 and 90 d. miRNA sequencing results showed that mir-122 was differentially expressed from d0 to d90 (6.72 log fold change; P = 0.0048) and from d0 to 124 (3.83 log fold change; P = 0.026). Several miRNA were down regulated (&gt; 2 log fold change; P &lt; 0.05) during TOF, which included miR-2411, -449a, -1197, -323, and -485. Relative expression of key lipogenic genes, stearoyl Co-A desaturase (SCD-1) and fatty acid synthase, were up-regulated (P &lt; 0.05) between d90 and 124 d. These results show that miR-122 appears to be a novel miRNA associated with marbling deposition and lipid metabolism.
207 Satellite Cells Participate in the wnt/ca2+ Pathway Controlled Porcine Myofiber Determination
Authors: Ye Mao , Song Zhiwen , Jin Chenglong , Gao Chunqi , Yan Huichao , Wang Xiu-qi 
Published: October 2021

Abstract
The type of myofiber is important for porcine meat quality. Meanwhile, the nt/Ca2+ pathway has been showed multiple roles in skeletal muscle formation; however, the distinct mechanism is still unclear. In this study, the weaned piglets and satellite cells were designed into the control group, lysine deficiency group and lysine rescue group to investigate the function of Wnt/Ca2+ pathway in governing skeletal muscle typing. After we confirm the growth of weaned piglets was controlled by lysine, the isobaric tag for relative and absolute quantification (iTRAQ) analysis of skeletal muscle detected that Wnt/Ca2+ pathway was involved in the transition of fast and slow fiber. Then, we found the ratio of type I myofiber in Semimembranous (fast muscle) was significantly increased after lysine deficiency (P &lt; 0.05), and decreased by lysine rescue (P &lt; 0.05). In contrast, the ratio of type I myofiber in Semitendinous muscle (slow muscle) was significantly decreased in the lysine deficiency group, and increased in the lysine rescue group (P &lt; 0.05). Furthermore, the Wnt/Ca2+ pathway was significantly increased in Semimembranous muscle, while decreased in Semitendinous muscle with lysine deficiency, and this phenomenon was inversed after lysine rescue (P &lt; 0.05). Meanwhile, the Wnt/Ca2+ pathway was stronger in satellite cells isolated from Semitendinous muscle (StSCs) than that of Semimembranous satellite cell (SmSCs) (P &lt; 0.05). And we also found the StSCs enter in differentiation is more easily than SmSCs (P &lt; 0.05). Besides, the ratio of type I myofiber originated from StSCs showed greater than StSCs (P &lt; 0.05). In summary, we conclude that satellite cells participate in the Wnt/Ca2+ pathway controlled porcine myofiber determination.
212 Fatty Acid Analysis and Composition in Meat by Mass Spectrometry
Authors: Dinh Thu 
Published: October 2021

Abstract
Fatty acids determine the physical and chemical properties of fats. Animal fats, regardless of species, have more saturated and monounsaturated than polyunsaturated fatty acids. The major fatty acids in meat are palmitic (16:0), stearic (18:0), palmitoleic (16:1), oleic (18:1), linoleic (18:2), and linolenic (18:3) acids, among which oleic acid is the most predominant. Arachidonic acid (20:4 cis 5,8,11,14) is an essential fatty acid only found in animal fats and can be used as a quality control indicator in the fatty acid analysis. Fatty acid analysis has been traditionally performed by gas chromatography (GC) of volatile fatty acid derivatives, prominently the methyl esters, and flame ionization detection (FID), in which the carbon chain of fatty acids is degraded to the formylium ion CHO+. The FID is very sensitive and is the most widely used detection method for GC, providing a linear response, i.e., peak area, over a wide range of concentrations. Researchers have been used the FID peak area to calculate the percentages of fatty acids. However, the FID is a “carbon counter” and relies on the “equal per carbon” rule; therefore, at the same molar concentration, fatty acids with a different number of carbons produce different peak areas. The recent development of mass spectrometry technology has improved the specificity of fatty acid detection. Specific target and qualifier ions provide better identification and more accurate quantification of fatty acid concentrations. Although fatty acids can be identified through comparing ion fragmentation with various databases, authentic standards are needed for quantification purposes. Using mass spectrometry, more than 50 fatty acids have been identified in meat samples. Some branched-chain fatty acids may have flavor, safety, and shelf life implications in meat products.
218 Replacement of Soybean Meal with Corn DDGS and Crystalline Amino Acids Impacts Performance and Carcass Characteristics of Finishing Pigs
Authors: Anderson Brooke E , Holt Jonathan P , Boyd R D , van Heugten Eric 
Published: October 2021

Abstract
This study evaluated the effects of replacing soybean meal (SBM) with DDGS and crystalline amino acids on growth, carcass lean, and carcass yield of finishing pigs. Pigs (n = 480; 83.1±0.35 kg) were blocked by BW and sex and assigned to 80 pens (3 gilts and 3 barrows/pen). Treatments were arranged as a 2×4 factorial with DDGS included at 0 or 20% and L-lysine·HCl (LYS) added at 0.0, 0.2, 0.4, or 0.6%. Diets were balanced for ideal protein and NE and contained 0.75% and 0.67% SID lysine for Phase 1 (21 days) and Phase 2 (to market, 13 or 20 days), respectively. Only 0.49% LYS was necessary to meet the SID lysine requirement for the highest LYS (0.6%) diet for Phase 2. As LYS increased, dietary SBM inclusion decreased from 21.75% to 2.85% (Phase 1) and 18.75% to 3.35% (Phase 2) for control diets. It decreased from 18.40% to 0% (Phase 1) and 15.40 to 0% (Phase 2) for DDGS diets. During Phase 1, DDGS decreased ADG (992 vs. 1039 g/d; P = 0.031) and ADFI (3424 vs. 3503 g/d; P = 0.061). Increasing LYS linearly decreased ADG (1031, 1037, 1035, 959 g/d; P=0.026) and G:F (299, 295, 298, 281 g/kg; P = 0.026). Treatments did not impact Phase 2 performance. Overall, DDGS reduced ADG (1098 vs. 1131 g/d; P = 0.048) and ADFI (3638 vs 3712 g/d; P = 0.070). Supplemental LYS linearly decreased ADG (1133, 1141, 1120, and 1064 g/d; P = 0.005) and G:F (310, 305, 304, and 295 g/kg; P = 0.006). ADFI increased quadratically (P = 0.002) with LYS within SBM control, but not DDGS (interaction; P = 0.009). Ultrasound loin-eye-area corrected for BW tended to decrease quadratically (48.3, 48.8, 49.1, 47.4 cm2; P = 0.060) with increasing LYS. DDGS reduced carcass yield (72.62 vs. 73.04%; P = 0.034). Replacement of SBM with DDGS and high amino acid inclusion negatively impacted growth performance and carcass yield of finisher pigs.
216 Effect of Dietary L-valine Supplementation on Growth Performance, Immune Response, and Microbial Metabolites in Weaned Pigs Raised in Varying Sanitary Conditions
Authors: Koo Bonjin , Nyachoti Martin 
Published: October 2021

Abstract
This study investigated the effects of dietary L-valine supplementation and sanitary conditions on growth performance, immune response, and microbial metabolites in weaned pigs. Thirty-two weaned pigs (6.98 ± 0.47 kg) were randomly assigned to treatments in a 2 × 2 factorial arrangement based on dietary Val level and sanitary condition (clean or unclean). The pigs were fed either a basal diet containing the standard levels of Val suggested by NRC (2012) or the basal diet with an additional 0.1% of L-Val. A room designated as the clean room was washed weekly, whereas the designated unclean room was not washed throughout the experiment and 5 kg of manure from the nursery pig barn was spread on the pen floors on day 1. All data were analyzed using a mixed procedure of SAS, with the individual pen as the experimental unit. The pigs raised in unsanitary conditions exhibited a lower average daily gain and average daily feed intake and a higher incidence of diarrhea than those raised in sanitary conditions during the 14-day experimental period (P &lt; 0.05). The pigs in the unclean group also had a lower concentration of butyrate in the jejunum and a higher concentration of ammonia-N in the colon than those in the clean group (P &lt; 0.05). Dietary valine supplementation reduced plasma interleukin (IL)-1α and IL-1 receptor antagonist concentrations as well as isovalerate and ammonia-N concentrations in the colon (P &lt; 0.05). Interactions between dietary Val supplementation and sanitary conditions were observed in the abundance of mRNA-encoding β-defensins 113, 125, and 129 (P &lt; 0.05). In conclusion, dietary Val supplementation beneficially modulates inflammatory response and microbial metabolites regardless of sanitary condition, while transcriptional levels of β-defensins are regulated by dietary Val supplementation in a manner dependent on housing hygiene conditions.
231 Applications of Insect-derived Ingredients in Animal Diets
Authors: Koutsos Liz 
Published: October 2021

Abstract
Insects have applications in many animal feeding programs for several reasons. First, increased emphasis on sustainable ingredients supports the use of insect-derived ingredients since insects have the capacity to sustainably re-purpose waste streams from food and feed production into nutrient dense ingredients. Second, many animals would naturally consume insects, thus incorporation into feeding programs may enhance animal welfare. Third, nutrient composition and availability is generally very high for insect-derived ingredients relative to requirements and formulae needs, although this varies with insect species, stage of development, feedstock on which they are raised and processing method. In addition to the essential nutrients that can be provided by feeding insect-derived ingredients, components including chitin and proteins and fatty acids with anti-microbial activity provide additional application in animal feeding programs. These concepts will be discussed relative to current knowledge about the primary insect species being reared at commercial scale: house crickets (Acheta domesticus), yellow mealworms (Tenebrio molitor), and black soldier fly larvae (Hermetia illucens).
229 Yeast Proteins Extracted from Saccharomyces Cerevisiae as Alternative Sources of Protein in Swine Nutrition
Authors: Kiros Tadele G , Nadege Richard , Marden Jean Philippe , Kuhn Geraldine , Catunda Fabio 
Published: October 2021

Abstract
Proteins obtained from microbial biomass like bacteria, yeast, and algae, coined as single cell proteins (SCP), are considered as new and unconventional sources of protein supply both for animal and human use. These microbes convert low value by-products and wastes into protein rich biomass through the process of fermentation. In this regard, yeast was the first microorganism used as source of protein in animal feed. Different industrial methods including plasmolysis, autolysis or enzyme digestions are used to extract the protein rich inner content of a yeast cell known as yeast extract (YE). The protein content of YE may vary depending on the method of extraction, but generally ranges between 50–60% and contains free amino acids and functional peptides. In swine the weaning transition is a critical period where piglets are exposed to different kinds of stresses including the abrupt change from milk to solid feed. To match the highly digestible and protein rich diet in milk, pig producers are using a highly complex diet containing animal products including spray-dried porcine plasma (SDPP) in the pre-starter diet. However, the use of animal products is under scrutiny for biosecurity reasons and SCP from yeast are emerging as good alternatives to replace animal products in piglet diets. Reports from various studies have shown proteins from YE can provide highly digestible proteins with good profile amino acids. Furthermore, it has been shown that yeast proteins improve health and performance of pigs by enhancing immunity and strengthening gut integrity of weaning piglets. Moreover, comparative animal studies in weaned piglets confirmed no significant difference between SDPP and YE proteins in energy content, AA composition, as well as apparent and standardized ileal digestibility of AA suggesting that SCP from yeast could be a good alternative to replace SDPP in weanling piglet diets.
245 Prenatal Transportation Stress Does Not Impact Ovarian Follicle Count in Brahman Offspring
Authors: Quail Lacey , Randel Ron D , Welsh Thomas H , Branco Rui d’Orey , Neuendorff Don A , Cushman Robert A , Yake Hannah , Perry George A 
Published: October 2021

Abstract
Calves from transported dams had greater concentrations of plasma cortisol when restrained and cleared plasma cortisol at a slower rate than calves from non-transported dams. Considering this hypothalamic-pituitary-adrenal axis effect, investigation of other parameters influencing reproduction is warranted in offspring exposed to prenatal transportation stress. The purpose was to determine impact of prenatal transportation stress on offspring ovarian follicle count. Brahman cows were transported for 2 h on d 60, 80, 120, and 140 (± 5 d) of gestation. Offspring from transported (Stressed, n = 19) or non-transported (Control, n = 15) dams were slaughtered at 5 yr (Replication 1, n = 14) or ovariectomized at 8 yr (Replication 2, n = 20). A cross-section of ovary was collected, serially sectioned, and stained. Numbers of total, primordial, primary, secondary, and antral follicles were determined per section. Total ovarian follicle count for each stage was calculated using ovary dimensions. The MIXED procedure of SAS was used to analyze ovarian follicle count with treatment, replicate, and the interaction as fixed effects. Total ovarian follicle count decreased with age (P &lt; 0.01; R1 = 383,663, R2 = 154,560); however, there was no difference in total ovarian follicle count between offspring due to treatment (P = 0.17; S = 221,813, C = 316,409). Similarly, there was no difference in primordial (P = 0.22; S = 127,300, C = 188,304), primary (P = 0.28; S = 58,610, C = 77,237), or antral (P = 0.48; S = 23,202, C = 28,695) follicle count between offspring due to treatment. Fewer secondary follicles were observed in Stressed offspring compared to Control offspring (P = 0.03). These results suggest that the ovarian follicular reserve, AFC, and potential fertility of cows may not be impacted by exposure to prenatal transportation stress. USDA is an equal opportunity provider and employer.
247 Metabolic Adaptation to Short-term of Dietary Nutrient Imbalances in Mid Lactation Dairy Cows
Authors: Gross Josef J , Stürmlin Remo , Bruckmaier Rupert M 
Published: October 2021

Abstract
This study investigated the short-term metabolic and endocrine responses to different levels of concentrate formulated to particularly provide one major macronutrient source. Based on parity number, lactational stage, milk yield and BW, 9 mid lactating cows (211 ± 19 d in milk) were grouped into 3 blocks of 3 animals each. Concentrate supplements (aminogenic: rich in crude protein and nitrogen sources; glucogenic: high content of carbohydrates and glucogenic precursors; lipogenic: high lipid content) were fed in a factorial arrangement at 3 increasing levels for 3 d each. Milk yield, BW and feed intake were recorded daily. Blood and milk were sampled every 3 d at the end of each concentrate level. Statistical analysis was carried out using a mixed model with concentrate supplement and level as fixed, and block and animal as random factors. Milk fat, protein, lactose and urea contents were determined. In blood, concentrations of various metabolites, endocrine factors and enzyme activities (e.g. glucose, non-esterified fatty acids (NEFA), β-hydroxybutyrate, urea, cholesterol, triglycerides, insulin, glucagon, ASAT, GGT, GLDH) were measured. Milk yield, milk composition and BW were not affected by diets and concentrate levels. Feed intake increased in cows receiving the aminogenic and lipogenic diet. Milk und plasma urea concentrations were clearly elevated in the aminogenic and to a lesser extent in the lipogenic treatment compared to the glycogenic diet (P &lt; 0.05). Glucose concentrations in plasma were not affected by treatments, whereas insulin and glucagon increased only in the aminogenic treatment with concomitantly reduced NEFA concentrations (P &lt; 0.05). Activities of ASAT, GGT and GLDH as well as the total antioxidant capacity were not affected by diets (P &gt;0.05). To conclude, immediate metabolic and endocrine responses occurred due to the short-term dietary changes. Particularly a surplus of nitrogen supply affected metabolic responses and stimulated insulin and glucagon secretion.
254 Effects of Nutrition on Bull Fertility
Authors: Geary Tom W , Dahlen Carl R , Zezeski Abigail L 
Published: October 2021

Abstract
The greatest effects of nutrition on bull fertility occur by providing a relatively high plane of nutrition during calfhood when the seminiferous epithelium of the testes is developing. A high plane of nutrition from 5 to 25 weeks of age results in greater circulating gonadotropin concentrations, greater testicular volume, and greater sperm production at maturity. At this age, diet can be manipulated by creep feeding bull calves with both protein and energy supplements which could accelerate puberty and increase sperm production. Increased rate of gain in bull calves post-weaning can accelerate puberty a few weeks, but excess post-weaning gain and fat buildup in the neck of the scrotum can have detrimental effects on fertility. Considerable emphasis has been placed on specific micronutrient (especially minerals) effects on peripubertal bull fertility. Trace mineral supplementation is believed to be critical for optimal fertility and both copper (Cu) and zinc (Zn) supplementation have influenced male fertility in other species. However, there are no guidelines for recommended levels of these minerals to ensure fertility. Providing organic or complexed compared to inorganic mineral to peripubertal bulls have increased liver concentrations of mineral and in some cases, have accelerated puberty, but enhanced fertility-associated measures have not been realized. Because both Cu and Zn are present in the ejaculate, mineral needs during the breeding season may differ from the off-season. During the breeding season, bulls can lose 10 to 20% of their weight and need to re-gain this weight before the next breeding season. Divergent planes of nutrition provided to mature bulls during the off-season revealed enhanced sperm energy and stress-fighting potential with decreasing plane of nutrition. Specific mineral supplementation during the off-season in mature bulls provides no beneficial effect on fertility-associated measures. In summary, significant effects of nutrition post-weaning on bull fertility-associated measures have not been realized.
253 Physiological Responses to Heat Stress of Holstein Heifers Carrying the SLICK1 Allele of the Prolactin Receptor (PRLR) Gene
Authors: Denicol Anna C , Carmickle Allie T , Pereira Jessica , Ferreira Fernanda C , Larson Colleen C , Sosa Froylan , Jansen Laura , Haimon McKenzie L , Hansen Peter J 
Published: October 2021

Abstract
The objectives of this study were to evaluate physiological responses to heat stress of Holstein heifers carrying the SLICK1 allele of the PRLR, i.e., the slick mutation. Slick and non-slick females were produced in central CA (n = 3 farms) and south FL (n = 3 farms) by inseminating cows with semen from two heterozygous slick Holstein sires. In July 2020, a subgroup of heifers of both genotypes (slick=93; non-slick=110) and two age groups (Group 1: 6–8 months old; n = 131; Group 2: 9–51 days old; n = 72) in both states were evaluated for rectal temperature (RT), surface temperature (ST) and sweating rate (SR). Dry bulb temperature, relative humidity and dew point were recorded every 15 min during testing for temperature humidity index (THI) calculation. Statistical models included the effects of genotype, state, farm within state, group, sire, THI, and interactions. Significant effects were considered as those with P &lt; 0.05. Although the THI in CA was lower than FL (80 ± 0.1 vs 84 ± 0.2; P &lt; 0.01), both were above 77 (estimated heat stress threshold for young animals). Slick heifers raised in FL had lower RT compared to non-slick siblings (39.6 ± 0.1 vs 40.1 ±0.1; genotype P &lt; 0.01); this difference was not observed in CA (39.1 ± 0.04 vs 39.1 ± 0.05; genotype P = 0.5; state x genotype P &lt; 0.01). In both states, slick heifers tended to have lower ST (34.7 ± 0.2 vs 35.1 ± 0.2; P = 0.07); CA heifers had lower RT and ST and higher SR (P &lt; 0.01) regardless of genotype. Current results show that presence of the SLICK1 allele can improve the ability of Holstein heifers to regulate body temperature, particularly in high humidity conditions. Next steps will include analysis of performance as study animals enter the reproductive phase. Funding sources: Holstein Association USA and L.E. “Red” Larson Endowment.
283 Precision versus Group Supplementation at Two Levels on Growth and Development of Replacement Heifers
Authors: Duckett Susan K , Cathcart Jacob , Cathcart Austin , Dantzler Zach , Dove Hunter , Andrae John G , Hersom Matt 
Published: October 2021

Abstract
Two experiments were conducted to evaluate use of the Super SmartFeeder (SSF; C-Lock, Inc.) for individual versus group supplementation of heifers grazing stockpiled, novel tall fescue. In experiment 1, Angus heifers (n = 64; 267 + 31.7 kg) had access to the SSF and were allowed 3.6 kg/d of grower supplement. Individual intake was recorded daily and analyzed to determine adoption. Twenty-four percent of the heifers did not adopt to individual SSF supplementation (P &lt; 0.05). In experiment 2, heifers (n = 64; BW= 275 + 31.3 kg) were allotted, based on adoption to SSF, to supplementation system of group feeding (n = 2 reps/level; GRP) or SSF precision feeding (n =16/level; PRE) at two levels (0.5% or 1% of BW) for 127-d in a 2 x 2 factorial. Data were analyzed using Mixed procedure. The interaction between supplementation level and feeding system tended to be significant (P = 0.10) for overall ADG. Average daily gain was greater for 1% BW than 0.5% BW (0.81 vs. 0.47 kg/d) for PRE but did not differ for GRP (0.69 kg/d). Daily supplement dry matter intake differed (P &lt; 0.05) by supplementation level and total BW gain was greater (P &lt; 0.05) by 24.3 kg for 1% versus 0.5% supplementation level. Ultrasound ribeye area and fat thickness measures were greater (P &lt; 0.05) for 1% BW supplement compared to 0.5% BW at the end of the 127d study. When PRE was analyzed independently, heifer BW differed (P &lt; 0.05) on d 91, 117 and 127 between supplement levels. The correlation between individual heifer daily supplement intake and overall ADG for PRE was 0.68 (P &lt; 0.0001). The use of technology to precisely control intake in a grazing system created greater divergence in growth by supplementation level compared to group feeding systems.