bluuebunny/crossref_metadata_2025_split huggingface dataset:
A transcription factor signature predicts the survival of patients with adrenocortical carcinoma
Authors: Zhao Jianyu , Liu Bo , Li Xiaoping 
Published: December 2021

Background
Adrenocortical carcinoma (ACC) is a rare endocrine cancer that manifests as abdominal masses and excessive steroid hormone levels and is associated with poor clinical outcomes. Transcription factors (TFs) deregulation is found to be involved in adrenocortical tumorigenesis and cancer progression. This study aimed to construct a TF-based prognostic signature for the prediction of survival of ACC patients.

Methods
The gene expression profile and clinical information for ACC patients were downloaded from The Cancer Genome Atlas (TCGA, training set) and Gene Expression Omnibus (GEO, validation set) datasets after obtained 1,639 human TFs from a previously published study. The univariate Cox regression analysis was applied to identify the survival-related TFs and the LASSO Cox regression was conducted to construct the TF signature based on these survival-associated TFs candidates. Then, multivariate analysis was used to reveal the independent prognostic factors. Furthermore, Gene Set Enrichment Analysis (GSEA) was performed to analyze the significance of the TFs constituting the prognostic signature.

Results
LASSO Cox regression and multivariate Cox regression identified a 13-TF prognostic signature comprised of CREB3L3, NR0B1, CENPA, FOXM1, E2F2, MYBL2, HOXC11, ZIC2, ZNF282, DNMT1, TCF3, ELK4, and KLF6. The risk score based on the TF signature could classify patients into low- and high-risk groups. Kaplan-Meier analyses showed that patients in the high-risk group had significantly shorter overall survival (OS) compared to the low-risk patients. Receiver operating characteristic (ROC) curves showed that the prognostic signature predicted the OS of ACC patients with good sensitivity and specificity both in the training set (AUC > 0.9) and the validation set (AUC > 0.7). Furthermore, the TF-risk score was an independent prognostic factor.

Conclusions
Taken together, we identified a 13-TF prognostic marker to predict OS in ACC patients.
Nanotheranostics of Pre‐Stenotic Vessels By Target Touch‐On Signaling of Peptide Navigator
Authors: Yu Seung Eun , Chung Seyong , Ha Hyun‐Su , Kim Dae‐Hyun , Baek Sewoom , Kim Tae Young , Lee Songhyun , Yoon Hyo‐Jin , Chung Soon Won , Lee Jung Bok , Shin Young Min , Kim Hye‐Seon , Kim Si Young , Park Joon‐Sang , Kim Chang‐Soo , Sung Hak‐Joon 
Published: December 2021

AbstractNavigation of nanoparticles to target sites of blood flow disturbance markedly upgrades the diagnostic paradigm in vascular medicine. The theranostic treatment of pre‐stenotic vessels can prevent the irreversible occlusion process effectively. Here, these nanotheranostic functions are established by displaying CDK9(cyclin‐dependent kinase 9)‐targeting peptide (P.) onto nanovesicles (NV) and liposomes using the navigation function and subsequent binding‐on signaling of P. as a game‐changer. When rabbit vessels are allografted with injecting contrast‐loaded P. liposomes, the case‐dependent stenotic degree after 2–6 weeks can be diagnosed accurately within 2–4 days via computed tomography imaging with cross‐validation in a mouse model of partial carotid ligation. Furthermore, the anti‐CDK9 signaling of P. NV is activated post‐targeting and effectively prevents vascular stenosis by suppressing inflammation and lipotoxicity in the vessels, serum, and/or liver. CDK9 targeting is confirmed using computer, in vitro, and in vivo models. This study demonstrates an unprecedented nanotheranostic function for future clinical applications.
Polysalicylic Acid Polymer Microparticle Decoys Therapeutically Treat Acute Respiratory Distress Syndrome
Authors: Brannon Emma R. , Kelley William J. , Newstead Michael W. , Banka Alison L. , Uhrich Kathryn E. , O'Connor Colleen E. , Standiford Theodore J. , Eniola‐Adefeso Omolola 
Published: December 2021

AbstractAcute lung injury (ALI) and acute respiratory distress syndrome (ARDS) remain problematic due to high mortality rates and lack of effective treatments. Neutrophilic injury contributes to mortality in ALI/ARDS. Here, technology for rapid ARDS intervention is developed and evaluated, where intravenous salicylic acid‐based polymer microparticles, i.e., Poly‐Aspirin (Poly‐A), interfere with neutrophils in blood, reducing lung neutrophil infiltration and injury in vivo in mouse models of ALI/ARDS. Importantly, Poly‐A particles reduce multiple inflammatory cytokines in the airway and bacterial load in the bloodstream in a live bacteria lung infection model of ARDS, drastically improving survival. It is observed that phagocytosis of the Poly‐A microparticles, with salicylic acid in the polymer backbone, alters the neutrophil surface expression of adhesion molecules, potentially contributing to their added therapeutic benefits. Given the proven safety profile of the microparticle degradation products—salicylic acid and adipic acid—it is anticipated that the Poly‐A particles represent a therapeutic strategy in ARDS with a rare opportunity for rapid clinical translation.
High‐Energy Aqueous Ammonium‐Ion Hybrid Supercapacitors
Authors: Chen Qiang , Jin Jialun , Song Mengda , Zhang Xiangyong , Li Hang , Zhang Jianli , Hou Guangya , Tang Yiping , Mai Liqiang , Zhou Liang 
Published: December 2021

AbstractThe development of novel electrochemical energy storage devices is a grand challenge. Here, an aqueous ammonium‐ion hybrid supercapacitor (A‐HSC), consisting of a layered δ‐MnO2 based cathode, an activated carbon cloth anode, and an aqueous (NH4)2SO4 electrolyte is developed. The aqueous A‐HSC demonstrates an ultrahigh areal capacitance of 1550 mF cm−2 with a wide voltage window of 2.0 V. An amenable peak areal energy density (861.2 μWh cm−2) and a decent capacitance retention (72.2% after 5000 cycles) are also achieved, surpassing traditional metal‐ion hybrid supercapacitors. Ex situ characterizations reveal that NH4+ intercalation/deintercalation in the layered δ‐MnO2 is accompanied by hydrogen bond formation/breaking. This work proposes a new paradigm for electrochemical energy storage.
3D Printed Skin‐Inspired Flexible Pressure Sensor with Gradient Porous Structure for Tunable High Sensitivity and Wide Linearity Range
Authors: Zhu Guotao , Dai Hongtao , Yao Yao , Tang Wenlai , Shi Jianping , Yang Jiquan , Zhu Liya 
Published: December 2021

AbstractAdvanced nanomaterials and novel microstructures have been developed to achieve flexible piezoresistive pressure sensors with high sensitivity and wide linearity range. However, the theoretical relationships between the sensitivity and the structure of the flexible pressure sensor have rarely been established. Consequently, the sensitivities of the sensors cannot be controlled and tailored for practical application. In this work, a skin‐inspired flexible pressure sensor with gradient porous structure is proposed. Based on the stress distribution of finite element analysis (FEA), the quantitative descriptions between the sensitivity and sensor structure parameters are derived. Then, carbon black (CB)/polydimethylsiloxane (PDMS) composites are successfully fabricated with desired gradient porous structures by 3D printing technology. Encouragingly, the skin‐inspired gradient structure sensor shows good sensitivity (0.0048 kPa−1) over the ultrawide linear range of 0–500 kPa. Meanwhile, the sensor shows excellent stability and durability during over 500 compression cycles. Taking advantage of these excellent sensing properties, the skin‐inspired gradient porous sensor is demonstrated in the application of voice recognition, grasp sensing, and intermittent pneumatic compression (IPC) interface pressure monitoring.
Comparative anatomy and ontogeny of appendicular skeleton of Pseudopus apodus (Pallas, 1775) (Anguimorpha, Anguidae) and a pattern of hindlimb loss in Anguinae
Authors: Klembara Jozef , Yaryhin Oleksandr , Majerová Jana , Hain Miroslav 
Published: December 2021

AbstractWe present a detailed anatomy of the pectoral girdles, pelvic girdles, and hindlimbs of adult and juvenile specimens of Pseudopus apodus (Pallas, 1775). We compared the individual bones of the appendicular skeleton of P. apodus with those of Anguis fragilis and species of Ophisaurus living in North America, North Africa, and Southeast Asia. We found no anatomical features in P. apodus in common with the species of Ophisaurus living in only North America, North Africa, and Southeast Asia. Additionally, we present the prehatching ontogeny of the pelvic girdle of P. apodus and A. fragilis and the prehatching ontogeny of the hindlimb of P. apodus. In the ontogeny of the pelvic girdle of P. apodus, it is possible to distinguish the ossification centers of ilium, ischium, and pubis. In contrast, in the ontogeny of A. fragilis, no ossification centers of ilium, ischium, and pubis are present, and no hindlimb element was detected. In Stage 1 of ontogeny in Pseudopus, the femur and tibia are present; in Stage 2, the nodule representing the fibula appears; and in Stage 3, in addition to the femur, tibia, and fibula, four tarsal elements are present. This anatomical condition corresponds to the anatomical composition of the hindlimb of the adult O. koellikeri. In Stage 4, the involution of all tarsal elements and fibula begins, and in the last two prehatching stages, only femur and tibia remain; this condition is present not only in the adults of Pseudopus, but also in those of several other species of Ophisaurus.
Unraveling the promise and limitations of CRISPR/Cas system in natural product research: Approaches and challenges
Authors: Biswas Protha , Anand Uttpal , Ghorai Mimosa , Pandey Devendra Kumar , Jha Niraj Kumar , Behl Tapan , Kumar Manoj , Chauhan Radha , Shekhawat Mahipal S , Dey Abhijit 
Published: December 2021

AbstractAn incredible array of natural products is produced by plants that serve several ecological functions, including protecting them from herbivores and microbes, attracting pollinators, and dispersing seeds. In addition to their obvious medical applications, natural products serve as flavoring agents, fragrances, and many other uses by humans. With the increasing demand for natural products and the development of various gene engineering systems, researchers are trying to modify the plant genome to increase the biosynthetic pathway of the compound of interest or blocking the pathway of unwanted compound synthesis. The clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 has had widespread success in genome editing due to the system's high efficiency, ease of use, and accuracy which revolutionized the genome editing system in living organisms. This study highlights the method of the CRISPR/Cas system, its application in different organisms including microbes, algae, fungi, and also higher plants in natural product research, and its shortcomings and future prospects.
In Vitro Schistosomicidal Activities of the Leaf Extracts from Casearia sylvestris Varieties
Authors: Carvalho Flávio A. , da Silva Breno C. , Oda Fernando B. , Junior Júlio C. P. , Crevelin Eduardo J. , Crotti Antônio E. M. , Caldas Lizandra G. M. , dos Santos André G. 
Published: December 2021

AbstractSchistosomiasis, a tropical disease caused by flatworms, may affect the liver, spleen, bladder, and intestine. Casearia sylvestris Swartz, a medicinal plant, displays antiprotozoal, antimicrobial, antifungal, and antiulcer activities. We have evaluated the in vitro schistosomicidal activity of two C. sylvestris varieties against Schistosoma mansoni adult worms at concentrations between 12.5 and 200 μg/mL. At 100 and 200 μg/mL, the ethanolic C. sylvestris var. sylvestris leaf extract enriched in casearin‐like diterpenes eliminated 100 % of the parasites after incubation for 72 h and 48 h, respectively, whilst the same extract at 200 μg/mL eliminated 96 %, 100 %, and 100 % of the parasites after incubation for 24, 48, and 72 h, respectively. On the other hand, the hydroalcoholic C. sylvestris var. lingua leaf extract at 200 μg/mL eliminated 60.4 and 66.7 % of the parasites after incubation for 48 and 72 h, respectively. The presence of casearin‐like diterpenes and glycosylated flavonoids was confirmed based on chromatographic techniques and mass spectrometry data.
Prebiotic Membranes and Micelles Do Not Inhibit Peptide Formation During Dehydration
Authors: Cohen Zachary R. , Kessenich Brennan L. , Hazra Avijit , Nguyen Julia , Johnson Richard S. , MacCoss Michael J. , Lalic Gojko , Black Roy A. , Keller Sarah L. 
Published: December 2021

AbstractCycles of dehydration and rehydration could have enabled formation of peptides and RNA in otherwise unfavorable conditions on the early Earth. Development of the first protocells would have hinged upon colocalization of these biopolymers with fatty acid membranes. Using atomic force microscopy, we find that a prebiotic fatty acid (decanoic acid) forms stacks of membranes after dehydration. Using LC‐MS‐MS (liquid chromatography‐tandem mass spectrometry) with isotope internal standards, we measure the rate of formation of serine dipeptides. We find that dipeptides form during dehydration at moderate temperatures (55 °C) at least as fast in the presence of decanoic acid membranes as in the absence of membranes. Our results are consistent with the hypothesis that protocells could have formed within evaporating environments on the early Earth.
Severe neurologic impairment and respiratory failure after fall and hip fracture in an elderly woman
Authors: Rostagno Carlo , Maiani Silvia , Di Donato Rosa , Bandini Giulia , Grassi Enrico , Fainardi Enrico 
Published: December 2021

AbstractFat embolism syndrome is a rare complication of long bone fractures and orthopedic surgery. We report a case of a 90‐year‐old woman who developed severe neurologic impairment and respiratory failure few hours after an accidental fall complicated by fracture of the left femur neck and left humerus.
Reversible blindness after erroneous prescription of closantel: A case report
Authors: Cheraghmakani Hamed , Jafari Reza , Karimpour‐razkenari Elahe , Ghazaeian Monireh 
Published: December 2021

AbstractA 20‐year‐old girl was referred with vision loss upon closantel use. Plasma exchange and high‐dose corticosteroid pulse therapy were administered. A 2.5‐year follow‐up showed improved vision and increased layer thickness of the peripheral nerve fiber. Early treatment with plasma exchange and high‐dose corticosteroid therapy can be beneficial to reverse closantel toxicity.
An eye‐catching atypical illustration of the evaluation and management of AL amyloidosis secondary to myeloma
Authors: Khan Shamis , Premji Sarah , Huang Quillan , Verstovsek Gordana , Bushan Sita , Yellapragada Sarvari Venkata 
Published: December 2021

AbstractWe present a case of a 58‐year‐old male patient who presented to his primary care clinic with complaints of eye swelling and fatigue. Workup ultimately led to a diagnosis of AL amyloidosis secondary to myeloma based on SLiM‐CRAB criteria. We discuss his diagnostic workup, treatment, and subsequent relapse.
Rehabilitation of ectodermal dysplasia patient with a telescopic denture in the maxilla and mandibular implant assisted overdenture: A case report
Authors: Alajami Heba , Saker Jamal 
Published: December 2021

AbstractHypohidrotic ectodermal dysplasia is a heritable disease, characterized by hypodontia, hypotrichosis, and anhidrosis. This clinical report demonstrates prosthetic rehabilitation of a patient complaining of impaired mastication and the odd appearance of her jaws. Maxillary telescopic overdenture and implant‐supported mandibular prostheses were fabricated to improve mastication, speech, and esthetics of the patient.
Nickel‐Catalyzed Reductive Allyl−Aryl Cross‐Electrophile Coupling via Allylic C−F Bond Activation
Authors: Fujita Takeshi , Kobayashi Yutaro , Takahashi Ikko , Morioka Ryutaro , Ichitsuka Tomohiro , Ichikawa Junji 
Published: December 2021

AbstractNickel‐catalyzed reductive cross‐coupling of allylic difluorides with aryl iodides was achieved via allylic C−F bond activation. Based on this protocol, a series of γ‐arylated monofluoroalkenes were synthesized in moderate to high yields with high Z‐selectivities. Mechanistic studies suggest that the C−I bonds of the aryl iodides and the C−F bonds of the allylic difluorides were cleaved via oxidative addition and β‐fluorine elimination, respectively, where the oxidative addition of less reactive C−F bonds was avoided to permit their transformation.
Olefin Metathesis in Confinement: Towards Covalent Organic Framework Scaffolds for Increased Macrocyclization Selectivity
Authors: Emmerling Sebastian T. , Ziegler Felix , Fischer Felix R. , Schoch Roland , Bauer Matthias , Plietker Bernd , Buchmeiser Michael R. , Lotsch Bettina V. 
Published: December 2021

AbstractCovalent organic frameworks (COFs) offer vast structural and chemical diversity enabling a wide and growing range of applications. While COFs are well‐established as heterogeneous catalysts, so far, their high and ordered porosity has scarcely been utilized to its full potential when it comes to spatially confined reactions in COF pores to alter the outcome of reactions. Here, we present a highly porous and crystalline, large‐pore COF as catalytic support in α,ω‐diene ring‐closing metathesis reactions, leading to increased macrocyclization selectivity. COF pore‐wall modification by immobilization of a Grubbs‐Hoveyda‐type catalyst via a mild silylation reaction provides a molecularly precise heterogeneous olefin metathesis catalyst. An increased macro(mono)cyclization (MMC) selectivity over oligomerization (O) for the heterogeneous COF‐catalyst (MMC:O=1.35) of up to 51 % compared to the homogeneous catalyst (MMC:O=0.90) was observed along with a substrate‐size dependency in selectivity, pointing to diffusion limitations induced by the pore confinement.
Piers′ Borane‐Induced Tetramerization of Arylacetylenes
Authors: Hasenbeck Max , Müller Tizian , Averdunk Arthur , Becker Jonathan , Gellrich Urs 
Published: December 2021

AbstractWe herein report that the reaction of Piers′ borane, i. e. HB(C6F5)2, with an excess of arylacetylenes at room temperature leads to tetramerization of the acetylene and the diastereoselective formation of boryl‐substituted tetra‐aryl‐tetrahydropentalenes. The reaction mechanism was investigated by isotope labeling experiments and DFT computations. These investigations indicate that a series of 1,2‐carboboration reactions form an octatetraene that undergoes an electrocyclization. Two skeletal rearrangements then presumably lead to the formation of the tetrahydropentalene core. Overall, this intricate and unprecedented transformation comprises five carbon‐carbon bond formations in a single reaction.
Enhancing Hydrogen Evolution by Optimizing the Hydrogen Adsorption on Titanium Monoxide Nanodot‐Decorated Cobalt Sulfide Nanosheets
Authors: Zhang Binyu , Zhou Yang , Cao Yang , Wang Xiaohong , Zhang Kexi , Tu Jinchun , Ding Lei 
Published: December 2021

AbstractModulating the local electronic state of metal compounds through interfacial interaction has become a key method for manufacturing high‐performance hydrogen evolution reaction (HER) electrocatalysts. The electron‐rich active sites can promote the adsorption of hydrogen, which accelerates the Volmer step and thereby enhances the electrocatalytic performance of HER. Here, we found that the strong interfacial interaction between TiO nanodots (TiO/Co−S) and Co−S nanosheets could advantageously improve the performance toward HER of electrocatalyst. Meanwhile, XPS results showed that modulating the local electronic structure of the TiO nanodots produces electron‐rich regions on Co. As a result, the overpotential of the TiO/Co−S nanocomposite at 10 mA cm−2 was 107 mV, and the Tafel slope was 83.3 mV dec−1. This study focused on the effect of the solid‐solid interface on the local electronic structure of the catalytic metal active sites and successfully improved the catalytic activity of transition metal materials in HER catalysis.
Smoke and heat accelerate and increase germination in fire‐prone temperate grassy ecosystems
Authors: Hodges Joshua A. , Price Jodi N. , Nicotra Adrienne B. , Neeman Teresa , Guja Lydia K. 
Published: December 2021

AbstractFire increases seedling recruitment by reducing competition for space and resources. As such, many species in fire‐prone ecosystems germinate in response to fire cues such as smoke and heat. A notable exception is fire‐prone temperate grassy ecosystems, where >20 yr of research has found that fire‐cued germination is rare. We tested the hypothesis that fire cues promote germination in temperate grassy ecosystems of south‐eastern Australia. We treated seeds of 55 common species with smoke, heat, and a combination of smoke and heat and tracked germination over time. We analyzed the effect of all combinations of treatments on germination speed and percent germination. Interestingly, we found that smoke and heat combined—which is more ecologically relevant to an actual fire than smoke and heat alone—was needed to increase germination speed, a mostly unstudied component of the germination ecology of grassy ecosystems. Smoke alone increased percent germination. Both plant family and seed traits influenced the germination response to smoke and heat. Poaceae species were the most responsive (in both speed and percent germination) to smoke alone. Water permeability of the seed coat was a key determinant of whether species responded to heat alone, smoke alone, or combined smoke and heat. Species with water‐impermeable seed coats responded to heat alone for both speed and percent germination. Species with water‐permeable seed coats germinated faster in response to combined smoke and heat and percent germination was increased by smoke alone. In contrast with more than two decades of research—our study of this large and representative group of species demonstrates that fire (via smoke and heat) is an important germination cue in fire‐prone temperate grassy ecosystems of south‐eastern Australia.
Fish carcasses alter subyearling Chinook salmon dispersal behavior and density but not growth in experimental mesocosms
Authors: Dunkle Matthew R. , Dunbeck Ryan A. , Caudill Christopher C. 
Published: December 2021

AbstractSimilar to many fish, juvenile Pacific salmon rearing in temperate streams face a growth and survival bottleneck early in their first summer due to limitations in space and food. Fish carcasses have been shown to affect juvenile salmon growth in many systems, but carcasses likely also alter behavioral and dispersal response of hatchery‐origin young‐of‐the‐year salmon. We tested the social and emigration response of subyearling chinook salmon to carcasses of Pacific lamprey or rainbow trout using semi‐open flow‐through mesocosm experiments. We expected the presence of carcasses to increase salmon growth and density while decreasing intraspecific aggression and downstream emigration compared to controls without carcasses. Consistent with expectations, salmon emigrated at lower rates when carcasses were present, resulting in significantly higher holding densities. Contrary to our expectations and results from previous studies, we did not detect differences in growth rate of juvenile salmon among treatments. Our findings suggest that indirect responses of consumers to concentrated resources may have measurable effects on density mediated by movement behaviors. Differences in lamprey and trout decomposition also suggest that carcass traits mediate the behavioral responses of consumers and incorporation of carcass subsidies into food webs.