bluuebunny/crossref_metadata_2025_split huggingface dataset:
Response of Kapok seedlings were irrigated with water of different qualities and heavy metal contents for foliar application of antioxidants
Authors: Abou-Sreea Alaa I. B. , Matter Faisal M. A. , Hassanain Mahmoud A. , Hassan Abdallah H. A. 
Published: January 2025

Abstract
Background
The imbalance between Egypt's water requirements and supply necessitates the use of unconventional water sources, such as treated sewage water (TSW) and agricultural drainage water (ADW), to combat water scarcity. This study investigated the effects of foliar glycine betaine (GB) on vegetative growth parameters, physiological characteristics, photosynthetic pigments, leaf element contents, anatomical leaf structures, and antioxidant activity. The experiment was conducted in two successive seasons (2021/2022 and 2022/2023) using Kapok seedlings irrigated with ADW and TSW at different mixing ratios with normal irrigation water (NIW) (25%, 50%, 75%, and 100%), combined with foliar spraying of GB at concentrations of 0.0 and 50 mM.

Results
The results revealed that irrigation with 100% TSW or ADW significantly decreased vegetative growth parameters, physiological characteristics, photosynthetic pigments, leaf element contents, leaf thickness, and the contents of the leaf mid-vein, N, P, K, and Ca. In contrast, the levels of free proline, total phenolic content, Na, Cu, Ni, Mn, Zn, Pb, and antioxidant activity increased. Additionally, GB significantly improved all parameters, while reducing the contents of Na, Cu, Ni, Mn, Zn, and Pb in the leaves.

Conclusions
Irrigation of Kapok seedlings with TSW or ADW mixed with NIW at 25% and 50% resulted in better performance, similar to irrigation with NIW alone for most parameters. Combining GB and water treatments by mixing TSW or ADW with NIW at a 50:50 ratio and spraying with 50 mM GB produced better results than control seedlings irrigated with 100% NIW. Antioxidants also play a defensive role in plants against various stress factors. Therefore, GB may have a protective effect on peroxidation-linked membrane deterioration, scavenge free radicals, and provide osmotic protection.
Beneficial mutualistic fungus Suillus luteus provided excellent buffering insurance in Scots pine defense responses under pathogen challenge at transcriptome level
Authors: Wen Zilan , Manninen Minna J. , Asiegbu Fred O. 
Published: January 2025

Abstract
Background
Mutualistic mycorrhiza fungi that live in symbiosis with plants facilitates nutrient and water acquisition, improving tree growth and performance. In this study, we evaluated the potential of mutualistic fungal inoculation to improve the growth and disease resistance of Scots pine (Pinus sylvestris L.) against the forest pathogen Heterobasidion annosum.

Results
In co-inoculation experiment, Scots pine seedlings were pre-inoculated with mutualistic beneficial fungus (Suillus luteus) prior to H. annosum infection. The result revealed that inoculation with beneficial fungus promoted plant root growth. Transcriptome analyses revealed that co-inoculated plants and plants inoculated with beneficial fungus shared some similarities in defense gene responses. However, pathogen infection alone had unique sets of genes encoding pathogenesis-related (PR) proteins, phenylpropanoid pathway/lignin biosynthesis, flavonoid biosynthesis, chalcone/stilbene biosynthesis, ethylene signaling pathway, JA signaling pathway, cell remodeling and growth, transporters, and fungal recognition. On the other hand, beneficial fungus inoculation repressed the expression of PR proteins, and other defense-related genes such as laccases, chalcone/stilbene synthases, terpene synthases, cytochrome P450s. The co-inoculated plants did not equally enhance the induction of PR genes, chalcone/stilbene biosynthesis, however genes related to cell wall growth, water and nutrient transporters, phenylpropanoid/lignin biosynthesis/flavonoid biosynthesis, and hormone signaling were induced.

Conclusion
S. luteus promoted mutualistic interaction by suppressing plant defense responses. Pre-inoculation of Scots pine seedlings with beneficial fungus S. luteus prior to pathogen challenge promoted primary root growth, as well as had a balancing buffering role in plant defense responses and cell growth at transcriptome level.
Prenatal diagnosis of mucopolysaccharidosis type I on hepatosplenomegaly and coarse features: a case-report
Authors: Agranier Maxime , Demurger Florence , Dubourg Christele , Fromageot Jerome , Dufour Anne-Sophie Cabaret , Launay Erika , Gournay Magalie , Lefèvre Charles , Froissart Roseline , Pettazzoni Magali , Rollier Paul 
Published: January 2025

Abstract
Background
Mucopolysaccharidosis type I (MPS I - IDUA gene) is a rare autosomal recessive lysosomal storage disorder. Clinical symptoms, including visceral overload, are progressive and typically begin postnatally. Descriptions of hepatosplenomegaly associated with lysosomal pathology are uncommon during the prenatal period. The most prevalent etiologies are infections, anemia, and neoplasms.

Case presentation
A pregnant woman at 26.5 gestational weeks was referred to our center for fetal ultrasound findings of hepatosplenomegaly, distinct facial features, and liver, spleen and thymus echogenic spots. Whole exome sequencing after amniocentesis identified two likely pathogenic IDUA gene variants (in trans), raising suspicion of a diagnosis of MPS I. MPS I was confirmed by the deficiency of α-L-iduronidase activity in amniotic cells. A medical pregnancy termination was carried out due to the severe prognosis. After termination of pregnancy, external examination of the fetus confirmed hepatosplenomegaly and coarse dysmorphic features.

Conclusion
Lysosomal storage diseases (LSD) are a rare cause of prenatal hepatosplenomegaly, but this has not been described in MPS I according to our literature search. The genetic variants identified in this case prompted early diagnosis through genome-wide studies. This rare presentation of MPS I highlights the expanding role of genomic analyses in diagnosing conditions during pregnancy.
Through-tunnel reconstruction with Ligament Augmentation and Reconstruction System (LARS) for managing post-arthroplasty patellofemoral instability
Authors: Chang Jung-Hsiu , Chen Chih-Hui , Li Yi-An 
Published: January 2025

Abstract
Background
Despite advancements in prosthetic designs and surgical techniques, patellar dislocation remains a rare but significant complication following total knee arthroplasty, with an incidence ranging between 0.15% and 0.5%. This condition often requires revision surgery to alleviate discomfort and restore joint function. Among the methods to address patellofemoral instability, medial patellofemoral ligament reconstruction has gained attention. In this case, we present the first report of using the synthetic Ligament Augmentation and Reconstruction System (LARS; Surgical Implants and Devices, Arc-sur-Tille, France) for medial patellofemoral ligament reconstruction following patellar dislocation after total knee arthroplasty, offering a novel approach to avoid donor site morbidity.

Case presentation
An 87-year-old man with no significant comorbidities underwent NexGen posterior-stabilized-total knee arthroplasty (Zimmer Biomet, Warsaw IN) for advanced osteoarthritis in his left knee. Three months postoperatively, he experienced a persistent giving-way sensation and swelling in the knee after a fall. Physical examination revealed patellar subluxation, confirmed by imaging studies. A computed tomography scan showed no malrotation of the femoral or tibial components. The patient was diagnosed with a medial patellar retinacular tear and medial patellofemoral ligament rupture. He underwent medial patellofemoral ligament reconstruction using a Ligament Augmentation and Reconstruction System (LARS; Surgical Implants and Devices, Arc-sur-Tille, France) synthetic ligament, and the medial retinaculum was repaired. After a year of follow-up, the patient reported satisfactory knee stability, with no recurrence of patellar dislocation.

Conclusion
This case demonstrates the successful use of a synthetic Ligament Augmentation and Reconstruction System for Medial patellofemoral ligament reconstruction in managing post-arthroplasty patellar dislocation. It offers a less invasive alternative to autograft harvesting, reducing donor site morbidity while providing effective stabilization of the extensor mechanism. This approach could have significant clinical implications, particularly for elderly patients with compromised bone healing capacity.
Crafting dental specialities in Iran: insights from a qualitative study
Authors: Rojhanian Tayebe , Aryan Kya Michael , Yazdani Shahram , Jadidfard Mohammad-Pooyan 
Published: January 2025

Abstract
Background
Different countries have varying dental specialities, shaped by diverse factors. The determinants influencing the development of these specialities differ between developed and developing countries. This study aimed to explore the factors contributing to the establishment of dental specialities in Iran, a developing country with a wide range of recognised dental specialities.

Methods
A qualitative case study was carried out, involving the review of 25 in-depth interviews and 47 documents. The data were organised using Atlas.ti (version 7.57) software and analysed through content analysis. This process included transcribing the interviews, identifying meaning units, abstracting content, categorising codes, and developing themes.

Results
The results identified three key factors influencing the development of dental specialities in Iran: stakeholders, contextual factors, and the specialisation process. Stakeholders encompass influential figures such as abroad-trained specialists, the government, the Ministry of Health and Medical Education, and scientific associations, along with their position, perceptions, and power. Contextual factors include cultural norms, sociopolitical relationships, political shifts, economic conditions, and academic disciplines. The process of establishing new dental specialities revealed several gaps, including the absence of formal needs assessments, advocacy plans, career planning, effective partnerships, and adequate evaluation mechanisms.

Conclusion
Contextual factors have played a crucial role in shaping dental specialisation in Iran, driving the formation of ideas in this field. Key players, including dentists trained abroad, have significantly influenced this process, often motivated by the desire to mirror practices in other countries. However, it did not address the specific oral health needs of the Iranian population. Due to limited awareness and the cost disparity between specialised and general services, there has been little public demand for dental specialisation. However, the process of establishing these specialities faces significant gaps that need to be addressed.
Sustainable reduction in sound levels on intensive care units through noise management - an implementation study
Authors: Witek Sandra , Schmoor Claudia , Montigel Fabian , Grotejohann Birgit , Ziegler Sven 
Published: January 2025

Abstract
Background
The noise levels in intensive care units usually exceed the recommended limits in (inter)national recommendations. Such noise levels can affect both the recovery of intensive care patients and the performance of staff. The aim of this study was to reduce ward-based noise levels in three intensive care units (anesthesiological, neurological, and neonatological).

Methods
The implementation of a setting-specific intervention bundle consisting of (a) ward-specific guide to noise management, (b) further noise reduction and prevention measures and (c) the use of “noise traffic lights” was evaluated in an implementation study with a pre-post design. Our primary endpoint was changes in sound level (equivalent continuous sound pressure (LAeq)) 12 weeks after the intervention, and the secondary endpoint was sound level (LAeq), peak sound pressure and maximum sound level at different time points, including changes at 24-week follow-up.

Results
After the intervention phase, we observed a significant overall reduction in the sound level of 0.77 decibels (A-weighted) (dB (A)), 95%-CI [0.06, 1.49], p = 0.034 with post-intervention measurements of LAeq1h 56.43 dB (A) compared to pre-intervention measurements of 57.21 dB (A). The difference was particularly large (2.21 dB (A) [p < 0.0001] in one of the three intensive care units. After adjusting our analysis for the intensity of nursing workload, the sound level reduction was smaller. Comparisons of LAeq1h between measurement times during the daytime periods showed a post-interventional difference of 58.28 dB(A) to 58.84 dB(A) baseline during the day of 0.57 dB(A), 95%-CI [-0.07, 1.21], p = 0.08 and at night of 53.36 dB(A) post-interventionally to 54.48 dB(A) baseline a difference of 1.11 dB(A) 95%-CI [0.19, 2.04], p = 0.02. In follow-up, baseline sound levels became realigned and we noted a rise in sound level of 0.81 dB (A) [p = 0.01].

Conclusions
Our implementation study indicates that a bundle of interventions can reduce noise levels in intensive care units, although the clinical relevance of the measured effect must be questioned. Sufficient resources and a participatory approach using an implementation framework should therefore be employed to manage sustainable noise abatement.

Trial registration
German Clinical Trials Register (DRKS): trial registration number: DRKS00025835; Date of registration: 12.08.2021.
Factors influencing trust among colleagues in hospital settings: a systematic review
Authors: Varga Andreea Isabela , Spehar Ivan , Veggeland Frode , Skirbekk Helge 
Published: January 2025

Abstract
Background
Many studies show positive results of collegial trust in the workplace, e.g. performance, innovation and collaboration. However, no systematic review on collegial trust in hospital settings exists. This study aimed to provide the missing overview of factors that positively and negatively influence this trust relationship between healthcare providers.

Methods
Ten information sources (Web of Science, Embase, MEDLINE, APA PsycInfo, CINAHL, Scopus, EconLit, Taylor & Francis Online, SAGE Journals and Springer Link) were searched from database inception up until October 21st, 2022. Empirical studies included were written in English, undertaken in a hospital or similar setting, and addressed collegial trust relationships between healthcare professionals, without date restrictions. Studies were excluded if they only explored trust between healthcare professionals on different hierarchical levels. Theoretical studies, systematic reviews, conceptually unclear papers and anecdotal case studies were also excluded. Records were independently screened for eligibility by at least two researchers. A narrative synthesis technique was adopted to explore and discuss the influencing factors of trust between colleagues identified across both quantitative and qualitative studies. This method was chosen given the inclusion of studies with different research designs and the unsuitability of the data for a meta-analysis or meta-ethnography. Risk of bias was assessed independently by at least two researchers using four critical appraisal tools.

Results
Eight thousand two hundred sixty-eight studies were screened and 11 studies were included. Seven were qualitative and four were quantitative. Themes identified were professional competence, elements of communication, such as tacit knowledge sharing, and ethical conduct, such as honesty, confidentiality and accountability. Moreover, trust among colleagues was seen to thrive in work environments characterised by psychological safety. The results of the quality assessment show that most studies were of an acceptable quality, with some associated risk of bias. One of the limitations was represented by the lack of a definition for trust in some studies, and some inconsistency for those studies that did define trust.

Conclusions
Professionalism, communication and ethics were seen as the most important factors enhancing trust. However, these concepts were defined differently in the studies.

Trial registration
PROSPERO; CRD42023433021.
Associations between class-level factors and student physical activity during physical education lessons in China
Authors: Zhou Yulan , Wang Lijuan , Chen Ruzhuan , Wang Bingnan 
Published: January 2025

Abstract
Background
The purpose of this study is to explore the association between class-level factors, such as lesson start time, class size, lesson location, PE content, and PE context, and student engagement in moderate-to-vigorous physical activity (MVPA) during PE lessons in both elementary and middle schools.

Methods
A total of 284 PE lessons from ten schools in Shanghai, Eastern China, were included in the study. Students’ MVPA during PE lessons was recorded using accelerometry, and lesson context was evaluated using the System for Observing Fitness Instruction Time (SOFIT). Mixed linear regression analysis was applied to assess the association between class-level factors and MVPA during elementary and middle school PE lessons.

Results
Students in elementary school spent 40.3 ± 8.1% of PE lesson time in MVPA, while middle school students spent 40.5 ± 7.1%. Significant relationships were found between MVPA and class-level factors like lesson location, PE content, and PE context. Specifically, elementary school students recorded a higher percentage of MVPA during lessons with team games, individual games, individual activities, and more time spent on skill practice and game play context. In middle schools, higher MVPA was connected to outdoor lessons, a focus on individual games, and more time devoted to fitness context.

Conclusions
Class-level factors may affect students’ MVPA differently depending on the school level, and these modifiable factors should be targeted to increase MVPA time in elementary and middle school PE classes. Future studies should investigate ways to modify these factors, strategically plan lesson time across different contexts, and optimizing PE content to boost MVPA in PE lessons.
Transmantle pressure under the influence of free breathing: non-invasive quantification of the aqueduct pressure gradient in healthy adults
Authors: Liu Pan , Owashi Kimi , Monnier Heimiri , Metanbou Serge , Capel Cyrille , Balédent Olivier 
Published: January 2025

Abstract
Background
The pressure gradient between the ventricles and the subarachnoid space (transmantle pressure) is crucial for understanding CSF circulation and the pathogenesis of certain neurodegenerative diseases. This pressure can be approximated by the pressure difference across the aqueduct (ΔP). Currently, no dedicated platform exists for quantifying ΔP, and no research has been conducted on the impact of breathing on ΔP. This study aims to develop a post-processing platform that balances accuracy and ease of use to quantify aqueduct resistance and, in combination with real-time phase contrast MRI, quantify ΔP driven by free breathing and cardiac activities.

Methods
Thirty-four healthy participants underwent 3D balanced fast field echo (BFFE) sequence and real-time phase contrast (RT-PC) imaging on a 3T scanner. We used the developed post-processing platform to analyse the BFFE images to quantify the aqueduct morphological parameters such as resistance. RT-PC data were then processed to quantify peak flow rates driven by cardiac and free breathing activity (Qc and Qb) in both directions. By multiplying these Q by resistance, ΔP driven by cardiac and breathing activity was obtained (ΔPc and ΔPb). The relationships between aqueduct resistance and flow rates and ΔP driven by cardiac and breathing activity were analysed, including a sex difference analysis.

Results
The aqueduct resistance was 78 ± 51 mPa·s/mm³. The peak-to-peak cardiac-driven ΔP (Sum of ΔPc+ and ΔPc−) was 24.2 ± 11.4 Pa, i.e., 0.18 ± 0.09 mmHg. The peak-to-peak breath-driven ΔP was 19 ± 14.4 Pa, i.e., 0.14 ± 0.11 mmHg. Males had a longer aqueduct than females (17.9 ± 3.1 mm vs. 15 ± 2.5 mm, p < 0.01) and a larger average diameter (2.0 ± 0.2 mm vs. 1.8 ± 0.3 mm, p = 0.024), but there was no gender difference in resistance values (p = 0.25). Aqueduct resistance was negatively correlated with stroke volume and the peak cardiac-driven flow (p < 0.05); however, there was no correlation between aqueduct resistance and breath-driven peak flow rate.

Conclusions
The highly automated post-processing software developed in this study effectively balances ease of use and accuracy for quantifying aqueduct resistance, providing technical support for future research on cerebral circulation physiology and the exploration of new clinical diagnostic methods. By integrating real-time phase contrast MRI, this study is the first to quantify the aqueduct pressure difference under the influence of free breathing. This provides an important physiological reference for further studies on the impact of breathing on transmantle pressure and cerebral circulation mechanisms.
SMORE: spatial motifs reveal patterns in cellular architecture of complex tissues
Authors: Samadi Zainalabedin , Hao Kai , Askary Amjad 
Published: January 2025

AbstractDeciphering the link between tissue architecture and function requires methods to identify and interpret patterns in spatial arrangement of cells. We present SMORE, an approach to detect patterns in sequential arrangements of cells and examine their associated gene expression specializations. Applied to retina, brain, and embryonic tissue maps, SMORE identifies novel spatial motifs, including one that offers a new mechanism of action for type 1b bipolar cells. Structural signatures detected by SMORE also form a basis for classifying tissues. Together, our method provides a new framework for uncovering spatial complexity in tissue organization and offers novel insights into tissue function.
Developing a knowledge transfer and exchange strategy for a clinical trials unit
Authors: South Annabelle , Terré Torras Berta , Rush Hannah , Goodman Anna , Love Sharon 
Published: January 2025

Abstract
Need for a strategic approach to knowledge transfer and exchange
Late-phase clinical trials and systematic reviews find results that have the potential to improve health outcomes for people. However, there are often delays in these results influencing clinical practice. We developed a knowledge transfer and exchange strategy to support research teams, aiming to identify activities along the research process to maximise and accelerate the research impact.

Our knowledge transfer and exchange strategy
The strategy has five strands of activity across the life-course of our research:
1. Partnerships with external stakeholders (including patient and public involvement, charities, policymakers, healthcare professionals, professional bodies, regulators and industry)
2. Communication (including the development of research impact strategies and use of communication tools and channels)
3. Maximising the scientific value of our studies (including open access, data and sample sharing, and incorporating multi-disciplinary components within studies)
4. Strengthening capacity (including building internal and partner capacity to communicate effectively, and strengthening the capacity of external stakeholders to understand and apply our research).
5. Learning and sharing (evaluating the impact of research, sharing lessons learnt internally and externally)
The strategy has helped trial teams think systematically about impact and was easy to use.

Conclusions
Our strategy helps researchers systematically identify activities which may improve the usefulness and uptake of their study results. While developed in a single trials unit, we think it may be of use to others.