bluuebunny/crossref_metadata_2025_split huggingface dataset:
Parendozoicomonas
Authors: Glaeser Stefanie P. , Kämpfer Peter 
Published: September 2021

Abstract

Par.en.do.zo.i.co.mo'nas. Gr. prep.
para
, beside; N.L. fem. n.
Endozoicomonas
, a bacterial genus; N.L. fem. n.
Parendozoicomonas
, beside the genus
Endozoicomonas
.

Proteobacteria / Gammaproteobacteria / Oceanospirillales / Endozoicomonadaceae / Parendozoicomonas

Cells are rod shaped and motile. Gram‐stain‐negative. Chemoorganotrophs with aerobic and anaerobic growth. Small whitish transparent colonies are formed on marine agar which turn orange after few days of incubation. Mesophilic and neutrophilic. Growth occurs between 8 and 37°C at a pH range of 6.5–9.0 with a growth optimum at 25–30°C and a pH of 7.0–8.0. Sodium chloride (at least 0.5% w/v) is required for growth. The fatty acid profile of the total cell hydrolysates consists mainly of two summed features comprising C
16:1
ω7
c
/C
16:1
ω6
c
and C
18:1
ω7
c
/C
18:1
ω6
c
and C
16:0
. The ubiquinone system consists mainly of ubiquinone Q‐9 and minor amounts of Q‐8 and Q‐10. The major polar lipids are phosphatidylethanolamine, phosphatidylglycerol, phosphatidylserine, and moderate amounts of diphosphatidylglycerol. Minor amounts of further unidentified phospholipids, amino lipid, and lipids, which are only visible after staining for total lipids, are also present. The polyamine pattern comprises the major compound spermidine and traces of putrescine, cadaverine,
sym
‐homospermidine, and spermine. The diagnostic diamino acid of the peptidoglycan is
meso
‐diaminopimelic acid. Phylogenetic analysis based on the 16S rRNA gene indicated that the genus represents a sister branch of the monophyletic genus
Endozoicomonas
within the family
Endozoicomonadaceae
, order
Oceanospirillales
, and class
Gammaproteobacteria
. Core genome‐based phylogeny confirmed the phylogenetic placement. The known habitat is the tissue of the marine sponge
Haliclona cnidata
.

DNA G + C content (mol%)
: 50.1–51.4 (
T
m
).

Type species
:
Parendozoicomonas haliclonae
Bartz et al. 2018, VL182.
Next generation pure component property estimation models: With and without machine learning techniques
Authors: Alshehri Abdulelah S. , Tula Anjan K. , You Fengqi , Gani Rafiqul 
Published: September 2021

AbstractPhysiochemical properties of pure components serve as the basis for the design and simulation of chemical products and processes. Models based on the molecular structural information of chemicals for the following 25 pure component properties are presented in this work: (critical‐) temperature, pressure, volume, acentric factor; (normal‐) boiling point, melting point, auto‐ignition temperature; flash point; (standard‐) enthalpy of formation, Gibbs energy of formation, enthalpy of fusion, enthalpy of vaporization, liquid molar volume; (environmental‐) (lethal dose‐) LC50 and LD50, photo‐chemical oxidation potential, bioconcentration factor, permissible exposure limit; (physicochemical‐) acid dissociation constant, water‐solubility, octanol–water partition coefficient, Hildebrandt solubility parameter, Hansen solubility parameters. Utilizing functional groups for molecular representation, two parallel property estimation models where the group contributions for each property are regressed through traditional regression techniques and machine learning techniques are presented. Both techniques use an a priori data analysis before regression of model parameters. A dataset with more than 24,000 chemicals for the 25 pure component properties has been utilized for the development of the two sets of property models. The efficacy of the developed models and their use are highlighted together with a discussion on the overall performance, application range, and predictive capabilities with implications to product and/or process engineering problem solutions.
Surfactant interactions and solvent phase solubility modulate small molecule release from emulsion electrospun fibers
Authors: Johnson Pamela M. , Lehtinen Justin M. , Robinson Jennifer L. 
Published: September 2021

AbstractEmulsion electrospinning provides a tunable system for the development of porous scaffolds for controlled, localized drug delivery in tissue engineering applications. This study aimed to elucidate the role of model drug interactions with emulsion chemistry on loading and release rates from fibers with controlled fiber diameter and fiber volume fraction. Nile Red and Rhodamine B were used as model drugs and encapsulation efficiency and release rates were determined from poly(caprolactone) (PCL) electrospun fibers spun either with no surfactant (Span 80), surfactant, or water‐in‐oil emulsions. Drug loading efficiency and release rates were modulated by both surfactant and aqueous internal phase in the emulsions as a function of drug molecule hydrophobicity. Overall, these results demonstrate the role of intermolecular interactions and drug phase solubility on the release from emulsion electrospun fibers and highlight the need to independently control these parameters when designing fibers for use as tunable drug delivery systems.
Airbrushed nanofibrous membranes to control stem cell infiltration in 3D‐printed scaffolds
Authors: Liaw Chya‐Yan , Huynh Shawn , Gedeon Christina , Ji Shen , D'souza Caroline , Abaci Alperen , Guvendiren Murat 
Published: September 2021

AbstractExtrusion‐based 3D printing of polymeric biomaterials has emerged as a promising approach for the fabrication of complex tissue engineering constructs. However, the large pore and feature size lead to low cell seeding efficiency and limited control of spatial distribution of cells within the scaffolds. We developed hybrid scaffolds that are composed of 3D‐printed layers and airbrushed fibrous membranes. Airbrushing time was adjusted to fabricate low (L), medium (M), and high (H) density membranes to effectively control stem cell infiltration. When two distinct populations of stem cells were seeded from top or bottom of the scaffolds, scaffolds composed of LLL membranes showed gradual mixing of the cells with depth, whereas LHL membranes led to two distinct regions of cells separated by the H membrane. Our results demonstrate that fibrous membranes incorporated within 3D‐printed layers enable user‐defined and spatially controlled cell compositions within hybrid scaffolds.
Synthesis of heat‐integrated pressure‐swing azeotropic distillation using a graphical pinch analysis
Authors: Lee Heecheon , Seo Chaeyeong , Lee Minyong , Lee Jae W. 
Published: September 2021

AbstractA new graphical method for the fundamental design of pressure‐swing distillation (PSD) and valid heat integration of the designed process is demonstrated based on a pinch analysis. As the minimum pressure constraint, the pinch pressure is determined by this graphical method to secure the necessary temperature driving force and circumvent the distillation boundaries. For a feasibility evaluation of heat‐integrated PSD (HIPSD), the suggested approach requires visualization of the pinch temperature contours and the feasible composition region of an initial feed according to the pressure change and dynamic properties of ternary azeotropic systems. We thus carried out an in‐depth examination by conducting case studies to show the feasible integration into HIPSD. The new insight is that the HIPSD system can have different pinch pressures depending on the column sequence or the separation order of pure components. In addition, the analysis verifies that energy‐efficient HIPSD can be generated by considering pinch constraints.
Impact of modernization on urinary concentrations of arsenic, cadmium, lead, and selenium in rural residents of Northern Laos
Authors: Mizuno Yuki , Masuoka Hiroaki , Kibe Mihoko , Kosaka Satoko , Natsuhara Kazumi , Hirayama Kazuhiro , Inthavong Nouhak , Kounnavong Sengchanh , Tomita Shinsuke , Umezaki Masahiro 
Published: September 2021

AbstractObjectivesThe impacts of modernization on toxic heavy metal exposure and essential trace element intake in indigenous populations of subsistence societies are unknown. We assessed urinary trace element concentrations in rural residents of Northern Laos and examined associations with levels of modernization.MethodsA cross‐sectional study was conducted comprising 380 residents of three villages in Northern Laos with different levels of modernization. We surveyed general characteristics and measured the weight and height of 341 participants. Arsenic, cadmium, lead, and selenium concentrations were measured in spot urine samples by inductively coupled plasma mass spectrometry. We examined associations between urinary trace element concentrations and variables related to modernization (village, roofing material, possessions index [total number of possessions], and body mass index [BMI]) using multilevel analyses with household as a random effect, after adjusting for sex, age, and smoking status.ResultsUrinary concentrations of arsenic and cadmium were high, while those of lead and selenium were low in comparison to previous reports of populations in non‐contaminated regions or without excess/deficiency. We observed associations between urinary trace element concentrations and village‐level modernization: lead and selenium concentrations were higher in more modernized villages and cadmium concentration was highest in the least modernized village. Urinary arsenic concentration was not predicted by the modernization level of a village, although we observed significant differences among villages. In addition, urinary selenium concentration was higher in participants inhabiting more modernized houses.ConclusionModernization of villages may impact toxic heavy metal exposure and selenium intake in rural residents of Northern Laos.
Dominant KPNA3 Mutations Cause Infantile‐Onset Hereditary Spastic Paraplegia
Authors: Schob Claudia , Hempel Maja , Safka Brozkova Dana , Jiang Huafang , Kim Soo Yeon , Batzir Nurit Assia , Orenstein Naama , Bierhals Tatjana , Johannsen Jessika , Uhrova Meszarosova Anna , Chae Jong‐Hee , Seeman Pavel , Woidy Mathias , Fang Fang , Kubisch Christian , Kindler Stefan , Denecke Jonas 
Published: September 2021

ObjectiveHereditary spastic paraplegia (HSP) is a highly heterogeneous neurologic disorder characterized by lower‐extremity spasticity. Here, we set out to determine the genetic basis of an autosomal dominant, pure, and infantile‐onset form of HSP in a cohort of 8 patients with a uniform clinical presentation.MethodsTrio whole‐exome sequencing was used in 5 index patients with infantile‐onset pure HSP to determine the genetic cause of disease. The functional impact of identified genetic variants was verified using bioinformatics and complementary cellular and biochemical assays.ResultsDistinct heterozygous KPNA3 missense variants were found to segregate with the clinical phenotype in 8 patients; in 4 of them KPNA3 variants had occurred de novo. Mutant karyopherin‐α3 proteins exhibited a variable pattern of altered expression level, subcellular distribution, and protein interaction.InterpretationOur genetic findings implicate heterozygous variants in KPNA3 as a novel cause for autosomal dominant, early‐onset, and pure HSP. Mutant karyopherin‐α3 proteins display varying deficits in molecular and cellular functions, thus, for the first time, implicating dysfunctional nucleocytoplasmic shuttling as a novel pathomechanism causing HSP. ANN NEUROL 2021;90:738–750
Efficacy and safety of anlotinib in patients with unresectable or metastatic bone sarcoma: A retrospective multiple institution study
Authors: Liu Zhiyong , Gao Songtao , Zhu Liangyu , Wang Jiaqiang , Zhang Peng , Li Po , Zhang Fan , Yao Weitao 
Published: September 2021

AbstractBackgroundTyrosine kinase inhibitors (TKIs) such as cabozantinib, regorafenib have demonstrated encouraging activity in prolonging progression‐free survival (PFS) in several bone sarcoma entities in prospective clinical trials. This retrospective study aims to analyze the safety and efficacy of anlotinib, a novel multi‐target TKI, in patients with locally unresectable or metastatic bone sarcoma at three institutions.MethodsPatients with advanced bone sarcoma administered anlotinib 12 mg once daily, 2 weeks on/1 week off, from June 2018 to June 2020, until disease progression or intolerance of treatment. The primary endpoints were objective response rate (ORR) and PFS.ResultsForty‐eight patients were analyzed: 27 have osteosarcoma, 9 have chondrosarcoma, 8 have Ewing's sarcoma, and 3 have chordoma. The median age was 24 years (range, 16–68 years), and the median number of prior regimens was 1 (range, 0–4). Until the final follow‐up, five patients obtained a partial response and while 24 achieved stable disease. The ORR in all patients was 10.4%, and the median PFS was 4.6 months, with a progression‐free rate (PFR) at 3 months and 6 months of 72.9% and 35.4%, respectively. The ORR and median PFS varied much among tumor subtypes. The most frequent grade 3–4 adverse events (AEs) were pneumothorax, hand‐foot syndrome, cholesterol elevation, hypertriglyceridemia, and fatigue. No patients died from anlotinib‐related AEs during the study period.ConclusionsAnlotinib may show promising antitumor activity in unresectable or metastatic bone sarcoma. The ORR and median PFS of anlotnib are similar to those of other targeted drugs in different subtypes of sarcomas. The AEs were generally mild and tolerated well. Further studies of anlotinib in selected subtypes of bone sarcoma are needed.
High mutations in fatty acid metabolism contribute to a better prognosis of small‐cell lung cancer patients treated with chemotherapy
Authors: Lyu Qiong , Zhu Weiliang , Wei Ting , Ding Weimin , Cao Manming , Wang Qiongyao , Guo Linlang , Luo Peng , Zhang Jian 
Published: September 2021

AbstractBackgroundThe majority of patients with small‐cell lung cancer (SCLC) show a good response in the early stages of treatment, but more than 90% of patients will develop drug resistance. Therefore, biomarkers are urgently needed to identify patients who can benefit from systemic treatment.MethodsWe prospectively enrolled 52 extensive‐stage SCLC patients before treatment from a local hospital to identify mutations related to patient prognosis, and verified them in the published Jiang's cohort and George's cohort.ResultsWe found that patients with high mutations (mut‐high) in the fatty acid (FA) metabolism pathway had a longer progression‐free survival (PFS) in the local hospital cohort (HR = 0.446, 95% CI, 0.207–0.959, p = 0.0387) and a longer overall survival (OS) in Jiang's cohort (HR = 0.549, 95% CI, 0.314–0.960, p = 0.0351) than patients with low mutations (mut‐low). Multivariate analysis suggested that mut‐high status was an independent prognostic factor in both cohorts. George's cohort verified that mut‐high status was associated with a longer OS than mut‐low status (HR = 0.730, 95% CI 0.440–1.220, p = 0.2277). The possible mechanisms were as follows: the frequency of mutated FA synthase (FASN) in the mut‐high group was greater than that in the mut‐low group, and pathways related to the cell cycle, DNA repair, and oxidative phosphorylation were enriched in the mut‐high group.ConclusionsThe prognosis of SCLC patients treated with chemotherapy was better among patients with more mutations in the FA metabolism pathway, and the underlying mechanisms could be found at the genome and transcriptome levels.
Salvage of a mangled limb with Matriderm® augmented split‐skin grafting and maggot biodebridement
Authors: Lempert Maximilian , Pape Hans‐Christoph , Jukema Gerrolt Nico 
Published: September 2021

AbstractSalvage of a mangled limb can be a long and strenuous way, but it is feasible even with rather simple techniques such as augmented split‐skin grafting and maggot biodebridement.
Endovascular management of symptomatic deep vein thrombosis with combined approach
Authors: Gallicchio Vito , Barbarisi Danilo , Sciarrillo Rosaria , Flora Loris 
Published: September 2021

AbstractThe combined used of thrombectomy with direct intravenous thrombolytic infusion provided effective treatment of DVT and uncovered an underlying left common iliac vein stenosis, which was successfully managed by angioplasty and stenting.
Difficult diagnosis of an air‐containing, abscess‐like, and mass‐like pancreatic head lesion
Authors: Xenou Antigoni , Vranou Eugenia , Boulas Konstantinos A. , Nathanailidou Maria , Kyriakidou Eytyxia , Sitaridis Konstantinos , Filippidis Isaac , Hatzigeorgiadis Anestis 
Published: September 2021

AbstractDuodenal diverticulosis can be a difficult CT diagnosis and should be considered in the differential diagnosis when a periduodenal mass‐like structure that may contain air, air‐fluid level, or oral contrast material is depicted.
Functional plasticity of carabids can presume better the changes in community composition than taxon‐based descriptors
Authors: Elek Zoltán , Růžičková Jana , Ódor Péter 
Published: September 2021

AbstractAlthough the functional trait approach can facilitate the understanding of mechanisms that underline community responses to habitat alteration, only a few studies used this way on exploring the structure of insect assemblages compared to taxon‐based analyses. We compared the descriptive power of medium‐term effects (2014–2018) of forestry treatments in a temperate managed oak‐dominated forest on taxon‐ vs. trait‐based descriptors of ground beetle assemblages. The treatments included rotation forestry (partial preparation cutting, clear‐cutting, retention‐tree group, and mature closed forest as control) and continuous cover forestry (gap cutting) operations. The species composition was only slightly influenced by the treatments; on the ordination biplot, the control, retention tree group, and clear‐cutting treatments formed relatively homogeneous groups, well separated from each other, while the others were scattered randomly in the ordination space. Over time, the species richness decreased in all treatments, but it was higher in the retention tree group treatment than in others in 2016 and 2017. The activity density also declined between years, but an immediate mass effect was revealed after the implementation of treatment types especially in the control, gap, and preparation cuts. We found that assemblages in the clear‐cutting and retention‐tree group had similar characteristics: high functional diversity; more open‐habitat, generalist, and omnivore species and fewer carnivore species; while those in the control, gap, and preparation cutting groups had the opposite: lower functional diversity, more forest species, and more carnivorous species. Our findings will demonstrate that the simultaneous use of the two approaches will allow the most articulate understanding of the status of ground beetles assemblages in managed forests.
SARS‐CoV2 pneumonia recovery is linked to expansion of innate lymphoid cells type 2 expressing CCR10
Authors: Gomes André M. C. , Farias Guilherme B. , Dias‐Silva Manuel , Laia Joel , Trombetta Amelia C. , Godinho‐Santos Ana , Rosmaninho Pedro , Santos Diana F. , Conceição Carolina M. , Costa‐Reis Renato , Adão‐Serrano Maria , Mota Catarina , Almeida Afonso R. M. , Sousa Ana E. , Fernandes Susana M. 
Published: September 2021

AbstractAccelerate lung repair in SARS‐CoV‐2 pneumonia is essential for pandemic handling. Innate lymphoid cells (ILCs) are likely players, given their role in mucosal protection and tissue homeostasis. We studied ILC subpopulations at two time points in a cohort of patients admitted in the hospital due to SARS‐CoV‐2 infection. COVID‐19 patients with moderate/severe respiratory failure featured profound depletion of circulating ILCs at hospital admission, in agreement with overall lymphocyte depletion. However, ILCs recovered in direct correlation with lung function improvement as measured by oxygenation index and in negative association with inflammatory and lung/endothelial damage markers like RAGE. While both ILC1 and ILC2 expanded, ILC2 showed the most striking phenotype changes, with CCR10 upregulation in strong correlation with these parameters. Overall, CCR10+ ILC2 emerge as relevant contributors to SARS‐CoV‐2 pneumonia recovery.
Hydroxychloroquine inhibits proteolytic processing of endogenous TLR7 protein in human primary plasmacytoid dendritic cells
Authors: Cenac Claire , Ducatez Mariette, F. , Guéry Jean‐Charles 
Published: September 2021

AbstractToll‐like receptor 7 (TLR7) triggers antiviral immune responses through its capacity to recognize ssRNA. Proteolytic cleavage of TLR7 protein is required for its functional maturation in the endosomal compartment. Structural studies demonstrated that the N‐ and C‐terminal domains of TLR7 are connected and involved in ligand binding after cleavage. Hydroxychloroquine (HCQ), an antimalarial drug, has been studied for its antiviral effects. HCQ increases pH in acidic organelles and has been reported to potently inhibit endosomal TLR activation. Whether HCQ can prevent endogenous TLR7 cleavage in primary immune cells, such as plasmacytoid DCs (pDCs), had never been examined. Here, using a validated anti‐TLR7 antibody suitable for biochemical detection of native TLR7 protein, we show that HCQ treatment of fresh PBMCs, CAL‐1 leukemic, and primary human pDCs inhibits TLR7 cleavage and results in accumulation of full‐length protein. As a consequence, we observe an inhibition of pDC activation in response to TLR7 stimulation with synthetic ligands and viruses including inactivated SARS‐CoV2, which we show herein activates pDCs through TLR7‐signaling. Together, our finding suggests that the major pathway by which HCQ inhibits ssRNA sensing by pDCs may rely on its capacity to inhibit endosomal acidification and the functional maturation of TLR7 protein.
Dryland maize yield potentials and constraints: A case study in western Kansas
Authors: Sun Shuang , Lin Xiaomao , Sassenrath Gretchen F. , Ciampitti Ignacio , Gowda Prasanna , Ye Qing , Yang Xiaoguang 
Published: September 2021

AbstractWater‐limited environments account for half of the earth's land surface, and dryland agriculture acreage is projected to expand due to climate changes. Examining typical dryland yield potentials and yield improvement measures is crucial for developing future dryland crop production systems. This case study used crop modeling to analyze dryland maize yield potential (YPd), farmers’ yield potential (YPf), and actual farm yields (Ya) from 1990 to 2015 in three counties in western Kansas (i.e., Thomas, Greeley, and Finney in the U.S. Great Plains region). The calibrated APSIM‐Maize model along with actual yields was used to estimate yield gaps attributed to: (a) agronomic factors (YG1 = YPd − YPf) and (b) socioeconomic constraints (YG2 = YPf − Ya). Observed climate conditions during maize‐growing seasons showed warming, brightening, and drying trends for all three counties in western Kansas from 1990 to 2015. Our results showed that the current actual farm yields (Ya) in western Kansas represented only 34%, 32%, and 28% of YPd in Thomas, Greeley, and Finney counties, respectively, indicating significant exploitable yield gaps. Agronomic factors (YG1) contributed the greatest to the yield gap in Greeley and Finney counties, whereas socioeconomic constraints (YG2) offered the greatest opportunity for improvement in Thomas county. Our analysis suggested that improving agronomic management could be a greater priority for further yield improvement in Greeley county, but selecting an appropriate hybrid was a greater priority in Finney county.