bluuebunny/crossref_metadata_2025_split huggingface dataset:
LncRNA HCP5 Encoding Protein Regulates Ferroptosis To Promote The Progression of Triple Negative Breast Cancer
Authors: Tong Xiao , Xing Jiani , Liu Haizhou , Zhou Shunheng , Huang Yue , Lin Jing , Yu Zhengling , Jiang Wei , Wang Lihong 
Published: August 2021

Abstract
Background

Long non-coding RNAs (lncRNAs) is widely described as a class of RNA longer than 200 nucleotides without encoding capability. But recent years, more and more open reading frames (ORFs) have been found in lncRNAs which indicate they have coding capacity. But the mechanisms of the encoding products in cancer are mostly unknown. We have previously shown lncRNA HCP5 is an oncogene in triple negative breast cancer (TNBC), and the aim of the current study was to investigate if lncRNA HCP5 encoding protein promotes TNBC by regulating ferroptosis.
Methods

We use bioinformatics to predict coding capacity. Molecular biology experiments and the xenograft assay in nude mice to study the mechanism of lncRNA HCP5 encoding protein. And the protein expression was evaluated in a tissue microarray of 140 invasive breast tumors and 45 pared precancerous breast tissues. Association between the protein expression and clinicopathologic features of breast cancer patients was analyzed.
Results

In this study, we identify that ORF in lncRNA HCP5 can encode a conserved protein with 132-amino acid. The protein, which is named HCP5-132aa, promotes TNBC growth. Mechanistically, the HCP5-132aa regulates GPX4 expression and lipid ROS level through ferroptosis pathway to promote TNBC progression. HCP5-132aa ORF knockdown synergizes with ferroptosis activators in vitro and in vivo. Breast cancer patients with high levels of HCP5-132aa have poorer prognosis.
Conclusions

Our study indicates that overexpression of lncRNA HCP5 encoding protein is a critical oncogenic event in TNBC. Our findings uncover a regulatory mechanism of ferroptosis in TNBC orchestrated by a protein encoded by an lncRNA.
The Prevalence of Non-suicidal Self-injury (NSSI): Protocol for a Systematic Review and Meta-analysis
Authors: Norozpour Marzieh , AghaMohammadi Soudeh , Taherifar Zahra , Zeinodini Zahra , Farahani Hanieh Shahrabi , Mousavi Nasim , Rostami Hanieh Goli , Keshtkar Abbas 
Published: August 2021

Abstract
Background: Non-suicidal self-injury (NSSI) is one of the most important public health concerns. NSSI usually begins in adolescence and is associated with several psychiatric, interpersonal, and intrapersonal problems. Prevalence estimates of NSSI has been reported globally and locally. However, the prevalence of NSSI even in similar populations is diverse in the literature. Most previous reports on NSSI prevalence originate from primary studies which usually have selection bias. Therefore, the objective of the current study is to estimate the prevalence of NSSI while considering the limitations of previous studies. Methods: All studies that have reported the prevalence of NSSI published from 1990 until the search date will be eligible to be included. This includes observational, survey, cohort, cross-sectional, and correlational studies. NSSI with any definition will be eligible to be included. There will be no language limitation. Special populations such as natives, minorities, or particular disorders will be excluded. Also, there will be no restriction regarding age, gender, nationality, and sexual orientation of the participants. To assess the methodological quality of the primary articles, a form will be used. Discussion: Considering the limitations of previous studies on the prevalence of NSSI, conducting a systematic review and meta-analysis to provide estimates of different types of NSSI. A better understanding of the prevalence of NSSI and related complications will point to gaps in research and help set preclude for future analyses. The results of this review will be applied by patients, healthcare providers, and policymakers.Systematic review registration: CRD42020180887
HN1 Promotes Proliferation, Metastasis and Attenuates the Chemosensitivity of HCC Cells to Oxaliplatin by Inhibiting Degradation of HMGB1 Through Interacting With TRIM28
Authors: Wang Ruhua , Fu Yunong , Yao Menglin , Cui Xiaomeng , Zhao Yan , Lu Xinlan , Li Yarui , He Shuixiang 
Published: August 2021

Abstract
Background: The oxaliplatin-based chemotherapy has revealed an encouraging therapeutic efficacy for advanced hepatocellular carcinoma patients. However, the development of resistance limits its clinical utilization. In addition, the chemotherapy resistance in HCC is usually accompanied with other malignant phenotypes, such as cell proliferation and metastasis, which together result in poor prognosis of HCC patients. Therefore, efforts should be made to explore potential regulators which fuel multiple events of HCC progression.Methods: The qRT-PCR, western blot, immunohistochemistry and immunofluorescence were performed to measure mRNA and protein expression. MTT assay, colony formation and Transwell assay were performed to evaluate cell proliferation and metastasis. Flow cytometry was performed to test cell apoptosis. Alkaline Comet assay was performed to measure DNA lesions. Transmission electron microscope analysis provided potent testimony of autophagy. The role of HN1 on the malignant phenotypes of hepatoma carcinoma was demonstrated in vitro and in vivo.Results: The immunohistochemistry analysis of HCC patient tissues revealed that the expression of HN1 was higher in HCC tissues compared to adjacent tissues and was associated with worse prognosis. In vitro, HN1 knockdown inhibited proliferation and metastasis of HCC cells, whereas HN1 overexpression promoted their proliferation and metastasis. In addition, we found that HN1 knockdown sensitized HCC cells to oxaliplatin, which is companied with deteriorated DNA damage and increased cell apoptosis in oxaliplatin-treated HCC cells. In vivo, HN1 knockdown inhibited the tumor growth and metastasis, and promoted the anti-cancer efficiency of oxaliplatin. Mechanically, HN1 prevented HMGB1 from ubiquitination and degradation via autophagy-lysosome pathway, which is related to its interaction with TRIM28, and overexpression of HMGB1 can restore the malignant phenotypes of HN1 knockdown in HCC cells. Furthermore, we found that HN1 can regulate cellular autophagy via HMGB1, which is important to tumor-promoting effect of HN1.Conclusions: In conclusion, we systemically revealed the multiple functions of HN1 in HCC progression and the underlying molecular mechanism, which indicated that HN1 could be a promising therapeutic target for HCC treatment.
Chitosan Fiber as Green Material for Removing Cr(VI) and Cu(II) Contaminants:Adsorption Properties,Kinetics and Machanism
Authors: Zhang shujie , Zhang Yating , Fu Lisong , Jing Mengke 
Published: August 2021

Abstract
Chitosan (CS) fiber is used as a new green material to remove Cu(II) and Cr(VI) in wastewater.Varying factors, including pH value, dosage of CS, reaction time and original Cr (VI) contents and Cu(II) were studied to investigate the Cr (VI) and Cu(II) removal efficiency.The adsorption of two metal ions by chitosan fiber conforms to the second-order kinetic equation, and can be fitted with Langmuir isotherms. The adsorption process is a spontaneous thermal reaction with both physical adsorption and chemical adsorption, and copper ions reach adsorption equilibrium. It takes longer than chromium ions, but the adsorption effect of copper ions is better. The maximum actual adsorption capacity of copper ions is 539.6 mg/g, and the maximum adsorption capacity of chromium ions is 75 mg/g. SEM, FTIR and XRD were used to characterize the physicochemical properties of CS fiber. The result shows that the complex process of the Cr (VI) and Cu(II) removal involves physical and chemical adsorption, CS fiber have exerted significant role in Cr (VI) and Cu(II) removal.
N-Bipolar Soft Sets and Their Application in Decision Making
Authors: Shabir Muhammad , Fatima Javaria 
Published: August 2021

Abstract
The concept of soft set was extended to $N$-soft set by Fatimah et al. and used as grading system. Bipolar soft sets gave the concept of a binary model of grading. Kamacı and Petchimuchu defined bipolar $N$-soft set but our approach is different from their approach. We defined N-bipolar soft set which extends the concept of bipolar soft set. We explained the notions through some important examples. We discussed some vital definitions and were motivated towards their use and need. We also described some basic algebraic definitions and with their help, we developed the algebraic structure of our proposed model. We give decision making algorithms and applied them to real life examples to motivate towards its application. Conflict analysis has been a vast topic for research. It was first given by Pawlak. The first extension to this model was given by Pawlak itself. Then many researchers extended his idea. We also discussed here the application of $N$-bipolar soft set to conflict analysis. The combination of $N$-bipolar soft set and conflict analysis can give user the best way to decide suitable and feasible action.
Insect Preservation Methods Allow Quantification of Dispersing Microorganisms by Scanning Electron Microscopy
Authors: Brummell Martin Earl 
Published: August 2021

Abstract
Insects may act as dispersal vectors for microbes where microbes can temporarily adhere to insect exoskeletons. Microbes carried by honey bees (Apis mellifera) may experience a range of dispersal outcomes, from successful colonisation of a new habitat to predation by grooming bees, partly depending on their location on the bee exoskeleton and the carried population size. I tested four methods for handling collected bees, and examined the bees in a scanning electron microscope, imaging and counting microbial cells attached to tarsal claws. Freeze-dried bees carried more microbial cells than bees that were pinned and air-dried, preserved in 96% ethanol then dried by CO2 critical-point drying, or bees washed with potassium phosphate then preserved in 96% ethanol, but some microbes were found on bees from every treatment. The similarity in microbial passengers found between air-dried and ethanol-preserved bees suggests that examination by electron microscopy could be used to address questions regarding microbial dispersal by pollinators already present in collections associated with other pollination research.
Spatial Patterns in the Association between the Prevalence of Asthma and Chronic Obstructive Pulmonary Disease: Socioeconomic and Climate Indicators in a Mediterranean Region
Authors: Bentué Carmen , Mimbrero Marcos Rodrigues , González Jose María Llorente , Ariño Antonio Sebastián , Martín Marcos Zuil , Antón María Zúñiga 
Published: August 2021

Abstract
The World Health Organization endorses the study of diseases from the perspective of the Determinants of Health (DH). DH are known as the circumstances in which people are born and raised, the environment in which they grow up and age and their lifestyle. The aim of this study is to analyze the spatial behavior of the prevalence of chronic obstructive pulmonary disease (COPD) and asthma in 2017 in Aragon, a Mediterranean region in Spain, using a DH approach. The methodological process entailed building a spatial database collating sociodemographic and climate indicators, and then evaluating the spatial variability of the relationships between DH indicators and disease prevalence by combining the Principal Component Analysis (PCA), Geographically Weighted Regression (GWR) models and cartographic design techniques. GWR evidences both global and spatially varying relationships, although each prevalence behaves differently. Asthma seems closely tied to local climate patterns whereas COPD is largely influenced by the built environment. Consequently, the socioeconomic and climatic contrasts characterizing the study area translate into several DH scenarios leading to situations vulnerable to the prevalence of asthma and COPD. This differential DH behavior detected by local regression models is relevant to guiding and refining public health decision-making.
Construction of Immune-related lncRNA Prognosis Models and Development Related Networks and Drugs in LGG
Authors: Zhang Xiaoyu , He Xiaoqin , Xiong Lin , Xu Yangtao , Chen Wenliang , Liu Xin , Hu Xinyao , Xu Ximing 
Published: August 2021

Abstract
Glioma is the most important tumor of the nervous system. LncRNA plays an important role in the occurrence, development, and metastasis of glioma. The immune status of glioma plays an important role in its treatment and prognosis. In lower-grade glioma (LGG), based on the results of the Weighted correlation network analysis (WCGNA) of lncRNA, we used lasso regression, random forest model, and stepwise regression to establish the lncRNA prognostic model: 0.58349*CYTOR + 0. 47804*LINC01831 + 0. 24933*HOTAIRM1 + 0. 73600*AC022034 + 0. 62351*AC104407 = lncRNA model prognosis score (LMPS). Concomitantly, we explored the correlation of LMPS with the Estimation of STromal and Immune cells in MAlignant Tumours using Expression data (ESTIMATE), Tumor IMmune Estimation Resource (TIMER) infiltrating immune cells. We also established the reciprocal network of the model and verified the significant prognostic significance of the hub genes of the network and the significant impact on immunity. Finally, by connectivity Map (cMap) analysis, we obtained the drugs about the WCGNA module where the model is. Our study highlights the close role of the lncRNA model with patient prognosis, immunity, and clinical staging, and can guide immunotherapy. The network is constructed with the model as the core. Its core genes also have a guiding role in patient prognosis, immunity, and treatment. We identified loratadine and fidaxomicin as possible drugs.
Anti-predation Defense Traits of Daphnia are Associated With the Gut Microbiota Composition Shaped by Fish Kairomone
Authors: Liu Qi , Akbar Siddiq , Ding Zihao , Zhou Qiming , Gu Lei , Sun Yunfei , Yang Zhou 
Published: August 2021

Abstract
Background: Gut microbiota plays an important role in host physiology and fitness. The gut microbiota can promote host health by influencing life history traits, especially in arthropods. However, it is not clear whether the performance of host defense traits in response to predator pressure in natural food webs is related to their gut microbiota composition. In this study, we used Daphnia magna as a model organism to investigate the relationship of D. magna life history traits and gut microbiota alterations under predator kairomone based on 16S rRNA amplicon sequencing.Result: We showed that the microbiota composition of D. magna was significantly affected by their predator risk and development stage. The relative abundance of Comamonadaceae (mainly Limnohabitans sp.) significantly decreased in the presence of predator kairomone. Furthermore, the presence of predator kairomone significantly reduced the α diversity of gut microbiota in D. magna with the increase of instar. Among them, the OTUs belonged to Epsilonbacteraeota and Firmicutes in the presence of predator kairomone were significantly higher than those in the control group. The results of functional predictions showed that predation pressure promote the metabolic function of gut microbiota, such as metabolism of energy, cofactors, and vitamins. By analyzing the correlation between the induced defense traits of D. magna and the relative abundance of bacteria, we found that the increased abundance of Comamonadaceae, Moraxellaceae, and Flavobacteriaceae were linearly correlated with the partial defense traits of D. magna. Specifically speaking, body size was positively correlated with an increased abundance of Comamonadaceae, whereas spine length was negatively correlated with an increased abundance of Comamonadaceae but was positively correlated with increased Flavobacteriaceae abundance. Conclusions: Our results suggested that predation risk can affect the composition of the gut microbiota in D. magna, which may indirectly induce the production of defensive traits in D. magna. The results of this study revealed an important role of gut microbiota in the development of defensive traits of Daphnia in response to fish predators. The correlation between microbial abundance and defense traits is of great significance for further understanding the effect of host-microbiota interaction on individual anti-predation defense.
Mobile-Learning App to Improving Skills in the Design of Therapeutic Exercise Program: Perception, Satisfaction and Physiotherapy Student´s Demand
Authors: Pastora-Bernal Jose-Manuel , Moreno-Morales Noelia , Estebanez-Pérez María-José , Molina-Torres Guadalupe , Fernandez-Navarro Rocío , Martín-Valero Rocío 
Published: August 2021

Abstract
Background: E-learning tools for students in health sciences present low levels of barriers and benefits for the student and the educator. No evidence is available using e-learning tools to design therapeutic exercise program skills. Research and different uses of technology in physiotherapy students are justified. The present study aimed to evaluate perception and satisfaction with an e-learning tool in five Spanish physiotherapy Universities. To evaluate the students' perception as an innovative learning experience identifying aspects to be improved and their perception respect to traditional teaching. Methods: A descriptive study in five health sciences faculties in Spain during the 2018-2020 academic years. The use of the tool is carried out under the supervision of physiotherapy teachers in diverse disciplines.Results: Results confirm that an e-learning tool to design therapeutic home exercise program is considered a satisfactory and innovative instrument that brings improvements in the learning of physiotherapy students with a high degree of satisfaction. Students declare aspect to be improved. All items have been valued positively with an average score of 3.41 in the set of 20 questions. It is considered a useful and necessary tool, compatible with traditional education. Conclusions: Our research provides new knowledge using innovative technologies enhancing learning skills. Students strongly recommend the use of the e-learning tool along the physiotherapy academic period.
Potential Value of the Calibrated Automated Thrombogram in Patients After a Cerebral Venous Sinus Thrombosis; an Exploratory Study
Authors: Bruggen Myrthe van der , Kremers Bram , Oerle Rene van , Oostenbrugge Robert Jan van , Cate Hugo ten 
Published: August 2021

Abstract
Background: Cerebral venous sinus thrombosis (CVST) is a relatively rare, but potentially lethal condition. In approximately 15% of the patients, the cause of CVST remains unclear. Conventional clotting tests such as prothrombin time and activated partial thromboplastin time are not sensitive enough to detect prothrombotic conditions nor mild haemostatic abnormalities. The calibrated automated thrombogram (CAT) is a physiological function test that might be able to detect minor aberrations in haemostasis. Therefore, we aimed to detect the presence of a prothrombotic state in patients who endured idiopathic CVST with the CAT assay. Methods: Adult patients with an idiopathic, radiologically proven CVST that had been admitted during the past 3 years were included in this study. The control group consisted of age/gender matched healthy volunteers. Exclusion criteria were known haematological disorders, malignancy (current/past) or hormonal and anticoagulant therapy recipients. We obtained venous blood samples from all participants following cessation of anticoagulation. Using the CAT assay, we determined lag time, normalized endogenous thrombin potential (ETP), ETP reduction and normalized peak height. In addition, prothrombin concentrations were determined. Results: We found no significant differences in lag time (4.7 min [4.5-4.9] vs 5.3 min [3.7-5.7], p = 0.691), normalized ETP (142% [124-148] vs 124% [88-138], p = 0.222), ETP reduction (29% [26-35] vs 28% [24-58], p >0.999), and normalized peak height (155% [153-175] vs 137 [94-154], p = 0.056) between patients and their age/gender matched controls. In addition, prothrombin concentrations did not significantly differ between patients and controls (120% [105-132] vs 127% [87-139], p>0.999.Conclusion: Reasons for absent overt hypercoagulability within this study population may be the small patient sample, long time since the event (e.g. 3 years) and avoidance of acquired risk factors like oral contraception. Given the fact that CVST is a serious condition with a more than negligible risk of venous thrombosis event recurrence, exclusion of clinically relevant hypercoagulability remains a challenging topic to further study at the acute and later time points, particularly in patients with idiopathic CVST.
Some electrophysical properties of polycrystalline silicon obtained in a solar oven
Authors: Olimov Lutfiddin Omanovich , Anarboyev Iqboljon 
Published: August 2021

Abstract
The article describes the results of the study of the microstructure and some electrophysical properties of silicon obtained by re-melting in a solar oven. It was found that the granularity of polycrystalline silicon consists of Si atoms with a size of 10–15 µm, the roughness of its surface. Decrease in specific resistance at T≤600 K, increase in concentration of ionized input atoms and concentration of charge carriers, the position at Т∼600 ÷ 700 K is based on the decrease in the free path of the charge carriers as a result of thermal vibrations of the crystal lattice, the situation at T ≥ 700 K K was explained by the emergence of new recombination centers specific to localized traps. Polycrystalline silicon heated by sunlight does not create a barrier effect of traps localized in the grain boundary regions from polycrystalline silicon obtained by other methods. This can expand the possibilities of creating highly efficient semiconductor devices, solar cells, thermoelectric materials for micro- and nanoelectronics, photovoltaics.
Sinusitis Independently Predicts the Presence of Headache Following Endoscopic Transsphenoidal Resection for Pituitary Adenomas
Authors: Gu Jiayu , Chen Xiaoqun , Zou Yunzhi , Yang Shuo , Chen Siyu , Li Depei , Sai Ke , Mou Yonggao , Zhou Zhihuan , Jiang Xiaobing 
Published: August 2021

Abstract
Purpose Headache is common among patients with pituitary adenomas undergone endoscopic endonasal surgery (EES), but was seldomly concerned before. The present study aims to investigate the incidence and profile of risk factors of headache after EES.Methods A meta-analysis was performed to evaluate the occurrence proportions of postoperative headache in patients with pituitary adenomas. Then, a cohort of 101 patients undergone EES were enrolled for analyzing risk factors of headache. The Headache Impact Test (HIT-6) was used to score the headache preoperatively, 1 month and 3 months postoperatively. Results A total of 18 studies and 4442 participants were included for meta-analysis. The pooled occurrence proportion of postoperative headache was 29% (95% confidential interval: 20-38%). For the 101 patients enrolled in the present study, 26 (25.74%) of them had a HIT-6 scores of > 55 preoperatively, but decreased to 22 (21.78%) at 1 month, and 6 (5.94%) at 3 months, postoperatively. Multivariate analysis showed that pituitary apoplexy (OR=3.591, 95%CI 1.219-10.575, p=0.020) and Hardy's grade C-D (OR=21.06, 95%CI 2.25-197.02, p=0.008) were independently risk factors for preoperative headache. In contrast, postoperative sinusitis (OR=3.88, 95%CI 1.16-13.03, P=0.028) and Hardy's grade C-D (OR=10.53, 95%CI 1.02-109.19, P=0.049) independently predicted the presence of postoperative headache at 1 month. At 3 months postoperatively, the proportion of sinusitis tended to be higher in the headache group than the one in non-headache group (100% vs. 30.0%, p=0.070). Conclusion Headache is very common following EES for pituitary adenomas. Prophylactic management of postoperative sinusitis may help to alleviate postoperative headache.