bluuebunny/crossref_metadata_2025_split huggingface dataset:
Role of biochemical parameters in the differential diagnosis of viable pregnancy, anembryonic pregnancy and intrauterine fetal exitus in cases of first trimester threatened abortion
Authors: Özolcay Ozan , Şık Bulat Aytek 
Published: July 2021

It is aimed to establish criteria about the prognosis and life potential of pregnancy by using ultrasonographic imaging techniques and hormonal parameters to evaluate embryonic life potantial in threatened miscarriage patients. Our study consists of 45 pregnant patients who were admitted for vaginal bleeding during pregnancy and were diagnosed as a threatened miscarriage. The study group consisted of pregnant women diagnosed with threat of miscarriage pregnancy week is lower than 20 weeks according to their last menstrual period. Estradiol, Progestrone, β-hCG hormone level are measured from patients, the same day after the ultrasound examination. The group whose pregnancy resulted with live birth and the group whose pregnancy ended with spontaneous abortion were statistically compared according to their hormonal parameters; it was found to be significantly lower in the group that resulted in live birth (p <0.01). Sensitivity, specificity, negative and positive predictive values of β-hCG, E2, P4 levels were found to be significant in patients with threatened miscarriage. Increase both time loss and correct diagnosis rate, after vaginal speculum examination the first choice in patients with abortus imminens is to request a transvaginal ultrasonographic examination and then assesment of hormonal parameters (β-hCG, progesterone, estradiol) if necessary.
​Extended English summary is in the end of Full Text PDF (TURKISH) file.
Özet

Düşük tehdidi olan hastalarda embriyonik yaşamın devamını değerlendirmek için ultrasonografik görüntüleme teknikleri ve hormonal belirteçleri kullanarak gebeliğin prognozu ve yaşam potansiyeli hakkında kriterler oluşturmak amaçlanmıştır. Çalışmamız, gebelikte vajinal kanama nedeniyle başvuran ve düşük tehdidi tanısı alan 45 gebe hastadan oluşmaktadır. Herhangi bir sistemik hastalığı bulunmayan ek bir jinekolojik patolojisi olmayan ve son adet tarihinden kesin olarak emin olan hastalar çalışma gruplarına dahil edildi. Çalışma grubu son adet tarihine göre 20 hafta ve daha küçük gestasyonel haftadaki düşük tehdidi tanısı alan gebelerden oluştu. Hastalardan Estradiol, Progestrone, β-hCG ölçümleri yapıldı. Gebeliği canlı doğum ile sonuçlanan grup ile gebeliği spontan abortusla sonlanan grubun β-hCG, P4, E2 degerleri istatiksel olarak karşılaştırıldığında; canlı doğum ile sonuçlanan grupta anlamlı olarak düşük bulunmuştur (p<0.01). Abortus imminensli hastalarda β-hCG, E2, P4 düzeylerinin sensivite spesifite, negatif ve pozitif prediktif değerleri anlamlı çıkmıştır. Abortus imminensli hastalarda vaginal muayene sonrası yapılacak ilk iş hem vakit kaybını hem de doğru tanı oranını arttırmak için ilk olarak transvajinal ultrasonografik inceleme ve ardından gerekirse hormonal parametreleri (β -hCG, progesteron, estradiol) istemek doğru olacaktır.
LOGISTIC MODEL DEVELOPMENT FOR PREDICTION OF COVID-19 INFECTED INDIVIDUALS: CASE STUDY IN CENTRAL JAVA PROVINCE, INDONESIA
Authors: Arditya Okky Widya , Widowati Widowati , Sutimin Sutimin , Tjahjana R. Heru , Sasongko Priyo Sidik 
Published: July 2021

In early 2020, covid-19 spread fast in the worldwide and cause the high death. The disease started from the Asian region which resulted in a viral pandemic in 2020. In order to anticipate the increasing of the cases, a strategy is needed to inhibit its transmission. The mathematical model approach is important tool for predicting of covid-19 spread in populations. In this paper we propose and analyze the dynamical behaviour of a developed logistic model by considering the effect of the contact patterns in reducing the covid-19 spread process. To verify the developed logistic model, numerical simulation was given with case study of covid-19 spread for patients under supervision in Central Java Province, Indonesia. Based on simulation results, it was found that physical distancing can reduce the growth of the covid-19 spread for patient under supervision. It can be seen from the number of covid-19 spread for patients under supervision with physical distancing intervention smaller compared to without physical distancing intervention.
Long noncoding RNA HULC contributes to paclitaxel resistance in ovarian cancer via miR-137/ITGB8 axis
Authors: Huang Bo , Wei Min , Hong Li 
Published: July 2021

Abstract
Long noncoding RNA (lncRNA) highly upregulated in liver cancer (HULC) has been reported to be implicated in chemoresistance. However, the potential mechanism of HULC in paclitaxel (PTX)-resistant ovarian cancer (OC) remains undefined. The expression of RNAs and proteins was measured by quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) and Western blot assay. The PTX resistance and apoptotic rate were assessed via 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and flow cytometry, respectively. Furthermore, the interaction between miR-137 and HULC or integrin beta-8 (ITGB8) was predicted by miRcode and starBase v2.0 and then verified by dual luciferase reporter and RNA pull-down assays. In addition, the xenograft mice model was established to explore the effects of HULC in vivo. HULC was significantly upregulated and miR-137 was downregulated in PTX-resistant OC tissues and cells. Also, the HULC depletion suppressed tumor growth and PTX resistance in PTX-treated mice. miR-137 was verified as a target of HULC and directly targeted ITGB8. And HULC knockdown downregulated ITGB8 expression by targeting miR-137. miR-137 inhibitor or ITGB8 overexpression mitigated the suppressive impacts of HULC knockdown on PTX resistance. Collectively, HULC modulated ITGB8 expression to promote PTX resistance of OC by sponging miR-137.
Prediction of compounds with antiosteoporosis activity in Chrysophyllum cainito L. leaves through in silico approach
Authors: Ma’arif Burhan , Fitri Hilwa , Saidah Nisfatul Lailatus , Najib Luqman Alfani , Yuwafi Achmad Hamdan , Atmaja Ria Ramadhani Dwi , Inayatillah Fidia Rizkiah , Dianti Meilina Ratna , Laswati Hening , Agil Mangestuti 
Published: July 2021

Abstract

Objectives
Estrogen deficiency causes various health problems in postmenopausal women, including osteoporosis. Phytoestrogen emerged as a potential alternative of estrogen with minimum side effects. The aims of this study were to analyze the metabolite profiling results of various extract of Chyrsophyllum cainito L. leaves, which contain phytoestrogen, through in silico study against 3OLS protein, an X-ray protein of ERβ, so it can predict the types of the phytoestrogen contents which have antiosteoporosis property.

Methods

In silico analysis was carried out for the compounds from the metabolite profiling data of C. cainito leaves from our previous study. The structure compounds from metabolite profiling results of various extract of C. cainito leaves were prepared with Avogadro 1.0.1 software, molecular docking was done using PyRx 0.8 software, and Biovia Discovery Studio Visualizer 2016 software was used to visualize the structure of compounds against 3OLS protein. The physicochemical characteristics of the compounds were analyzed using the SwissADME web tool.

Results
From in silico studies, it was known that there were total 11 compounds in C. cainito leaves that predicted as phytoestrogens which have ERβ agonist properties against 3OLS protein. The ERβ agonist was a compound that has parameters similar to 17β-estradiol in its interaction with 3OLS protein, which has a pharmacophore distance of 10.862 Å, and binding to amino acids His 475 and Glu 305 or Arg 346 at receptor-ligand docking simulation.

Conclusions

C. cainito leaves contain 11 compounds that are predicted to be phytoestrogens with ERβ agonist properties, which is responsible for antiosteoporosis activity.
Artocarpus sericicarpus stem bark contains antimalarial substances against Plasmodium falciparum
Authors: Tumewu Lidya , A’yun Lutfah Qurrota , Ilmi Hilkatul , Hafid Achmad Fuad , Widyawaruyanti Aty 
Published: July 2021

Abstract

Objectives
The finding of alternative medicine for malarial treatment still has become a substantial demand. The plant is one of the potential sources of drugs, among other natural sources. Artocarpus species showed great potential as the antimalarial source. This study aims to obtain active antimalarial fractions from Artocarpus sericicarpus stem bark.

Methods
Stem bark of A. sericicarpus was extracted by ultrasonic-assisted extraction method using n-hexane, dichloromethane, and methanol as solvents. Fractionation of dichloromethane extract was conducted by open column chromatography using octadecyl silica as a stationary phase and gradient acetonitrile-water as a mobile phase. The antimalarial activity was determined by lactate dehydrogenase (LDH) assay against Plasmodium falciparum 3D7 strain.

Results

A. sericicarpus n-hexane, dichloromethane, and methanol extracts were showed antimalarial activity with an IC50 value of >4, 2.11, and >4 μg/mL, respectively. Fractionation of dichloromethane extract was obtained 13 fractions. Seven of the 13 fractions tested showed antimalarial activity. Fraction-6 performed the highest inhibition with an IC50 value of 1.53 ± 0.04 μg/mL. Phytochemistry screening revealed that Fraction-6 contains flavonoid, polyphenol, and terpenoid compounds that can take a role in its antimalarial activity.

Conclusions

A. sericicarpus contains antimalarial substances mainly in Fraction-6, which strongly inhibited the growth of P. falciparum. The flavonoid, polyphenol, and terpenoid compounds were identified in Fraction-6, which need to be further isolated to obtain and elucidate the active antimalarial compounds.
The stability and irritability study of the chitosan–Aloe vera spray gel as wound healing
Authors: Retnowati Dini , Sari Retno , Hendradi Esti , Septiani Septiani 
Published: July 2021

Abstract

Objectives
Chitosan is a natural polysaccharide widely used in various clinical applications including regeneration of skin tissue. Aloe vera has properties in healing burns on the skin, anti-inflammatory effect, and leaves a protective layer on the skin after drying so it provides protection to the wound. The spray gel of chitosan–A. vera was developed as a wound healing that has combined of effect of both component and easy to use. The purpose of this study was to determine the physical stability and irritability of chitosan–A. vera spray gel.

Methods
The spray gel stability test was conducted using thermal cycling and centrifugation methods. The organoleptic, viscosity, and pH of the spray were evaluated. The irritation test was performed by Draize Rabbit Test method.

Results
Chitosan (0.5%)–A. vera (1%) spray gel characteristics has a weak yellow color, clear, and a strong A. vera odor. The pH of the spray gel was 4.88 ± 0.01; and the viscosity was 36.50 ± 0.23 cps. The result from the chitosan (0.5%)–A. vera (1%) spray gel stability test using thermal cycling method showed a decrease of viscosity, but remained stable when evaluated using centrifugation method. There was no difference in the pH and organoleptic observation from both tests. Based on the scoring and analysis of the reaction in rabbit skin, the Primary Irritation Index (PII) obtained was 0.56.

Conclusions
The spray gel of chitosan (0.5%)–A. vera (1%) was stable and according to response category from the acute dermal irritation test, it can be concluded that chitosan (0.5%)–A. vera (1%) spray gel had a slightly irritating effect.
Fabrication and characterization of bovine hydroxyapatite-gelatin-alendronate scaffold cross-linked by glutaraldehyde for bone regeneration
Authors: Samirah  , Budiatin Aniek Setiya , Mahyudin Ferdiansyah , Khotib Junaidi 
Published: July 2021

Abstract

Objectives
Alendronate are widely used in the treatment of bone disorders characterized by inhibit osteoclast-mediated bone resorption such as Paget’s disease, fibrous dysplasia, myeloma, bone metastases and osteoporosis. In recent studies alendronate improves proliferation and differentiation of osteoblasts, thereby facilitating for bone regeneration. The disadvantages of this class are their poor bioavailability and side effects on oral and intravenous application such as stomach irritation and osteonecrosis in jaw. Thus, local treatment of alendronate is needed in order to achieve high concentration of drug. Bovine hydroxyapatite-gelatin scaffold with alendronate was studied. Glutaraldehyde was used as cross-linking agent, increase the characteristics of this scaffold. The objectives of this study were to manufacture and characterize alendronate scaffold using bovine hydroxyapatite-gelatin and crosslinked by glutaraldehyde.

Methods
Preparation of cross-linked bovine hydroxyapatite-gelatin and alendronate scaffold with different concentration of glutaraldehyde (0.00, 0.50, 0.75, and 1.00%). The scaffolds were characterized for compressive strength, porosity, density, swelling ratio, in vitro degradation, and cytotoxicity (the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide assay, shorted as MTT assay).

Results
Bovine hydroxyapatite-gelatin-alendronate scaffold cross-linked with glutaraldehyde showed lower density than without glutaraldehyde. As glutaraldehyde concentration increased, porosity also increased. Eventually, it reduced compressive strength. Swelling ratio and in vitro degradation was negatively dependent on glutaraldehyde concentration. In addition, the scaffold has a good safety by MTT assay.

Conclusions
Bovine hydroxyapatite-gelatin-alendronate scaffold was fabricated with various concentrations of glutaraldehyde. The presence of glutaraldehyde on bovine hydroxyapatite-gelatin-alendronate is safe and suitable candidate scaffold for bone regeneration.
The effects of quercetin on the expression of SREBP-1c mRNA in high-fat diet-induced NAFLD in mice
Authors: Saleh Al-maamari Jamal Nasser , Rahmadi Mahardian , Panggono Sisca Melani , Prameswari Devita Ardina , Pratiwi Eka Dewi , Ardianto Chrismawan , Balan Santhra Segaran , Suprapti Budi 
Published: July 2021

Abstract

Objectives
The study aimed to determine the effect of quercetin on the expression of primary regulator gene involved in lipogenesis and triglycerides synthesis in the liver, and the sterol regulatory binding protein-1c (SREBP-1c) mRNA in non-alcoholic fatty liver disease (NAFLD) with a high-fat diet (HFD) model.

Methods
Fifty-six Balb/c mice were divided into seven groups: standard feed; HFD; HFD and quercetin 50 mg/kg for 28 days; HFD and quercetin 100 mg/kg BW for 28 days; HFD and quercetin 50 mg/kg for 14 days; HFD and quercetin 100 mg/kg for 14 days; HFD and repaired fed for 14 days. Quercetin was administered intraperitoneally. The animals were sacrificed 24 h after the last treatment; the liver was taken for macroscopic, histopathological staining using hematoxylin–eosin and reverse transcription-PCR analysis sample.

Results
HFD significantly increased the expression of SREBP-1c mRNA; meanwhile, quercetin and repaired feed significantly reduced the expression of SREBP-1c mRNA in the liver. Quercetin at a dose of 50 mg/kg and 100 mg/kg also improved liver cells’ pathological profile in high-fat diet NAFLD.

Conclusions
The present study suggests that quercetin has an inhibitory effect on SREBP-1c expression and improved liver pathology in NAFLD mice.
Gastroprotective effect of fluvoxamine and ondansetron on stress-induced gastric ulcers in mice
Authors: Rahmadi Mahardian , Su’aida Nily , Yustisari Pratiwi , Agung Dewaandika Wahyu , Oktavia Hanaratri Elma , Andarsari Mareta Rindang , Sumarno  , Aryani Toetik 
Published: July 2021

Abstract

Objectives
The association between stress and gastric ulcers has been well reported. This study is divided into two parts: the first part of this study is consisted of analyzing the effect of fluvoxamine administration by intracerebroventricular (ICV) and intraperitoneal (IP) injections on stress-induced gastric ulcers. The second part investigates the effect of ondansetron in influencing the protection of the gastric mucous by giving fluvoxamine to the mice before being induced with stress.

Methods
Water immersion restraint stress (WIRS) was used to induce stress. Fluvoxamine 50 and 100 mg/kg by IP injection, fluvoxamine 9.3 µg, and 18.6 µg by ICV injection 30 min before the induction of stress. Meanwhile, single drug and in combination administered to the mice, ondansetron 3 mg/kg was given by IP at 60 min, and fluvoxamine 50, 100 mg/kg orally at 30 min before stress induction.

Results
The obtained results show fluvoxamine 50 and 100 mg/kg by IP, and fluvoxamine 18.6 µg by ICV had significantly reduced ulcer index with p<0.005, p<0.001, and p<0.005 while fluvoxamine 9.3 µg showed the insignificant result. Fluvoxamine 50 mg/kg, fluvoxamine 100 mg/kg, and ondansetron 3 mg/kg monotherapy have a significant reduction in ulcers with p<0.005, p<0.001, and p<0.05, while the combination drugs showed an insignificant reduction in ulcers.

Conclusions
Fluvoxamine with different administration routes and ondansetron monotherapy before stress reduce the occurrence of gastric ulcers, while the combination drugs did not increase the protective effect of the gastric mucosa.
Molecular docking studies of Nigella sativa L and Curcuma xanthorrhiza Roxb secondary metabolites against histamine N-methyltransferase with their ADMET prediction
Authors: Nurhan Ahmad Dzulfikri , Gani Maria Apriliani , Budiatin Aniek Setiya , Siswodihardjo Siswandono , Khotib Junaidi 
Published: July 2021

Abstract

Objectives
Histamine N-methyltransferase (HNMT) is an enzyme that plays a crucial role in the inactivation of histamine in central nervous system, kidneys and bronchi. Inhibition of HNMT is known to have a potential role in treating attention-deficit hyperactivity disorder, memory impairment, mental illness and neurodegenerative illnesses. Therefore, to find potential compounds that could be developed as novel HNMT inhibitors, this study conducted an in silico study of the secondary metabolites of Nigella sativa L and Curcuma xanthorrhiza Roxb.

Methods
In this study, we conducted a molecular docking study of 36 secondary metabolites of N. sativa L and 26 secondary metabolites of C. xanthorrhiza Roxb using an in silico approach targeting HNMT protein (PDB ID: 2AOT) using AutoDockVina software. The prediction of ADMET characteristics was done using the pkCSM Online Tool.

Results
This study obtained one metabolite from N. sativa L (longifolene) and seven metabolites from C. xanthorrhiza Roxb {(+)-beta-atlantone, humulene epoxide, (−)-beta-curcumene, (E)-caryophyllene, germacrone, (R)-(−)-xanthorrhizol, and (−)-beta-caryophyllene epoxide} which were predicted to have potential to be developed as HNMT inhibitors.

Conclusions
This study found several secondary metabolites of N. sativa L and C. xanthorrhiza Roxb which had activity as HNMT inhibitors. This research can likewise be utilized as a basis for further research, both in vitro, in vivo, and clinical trials related to the development of secondary metabolites from N. sativa L and C. xanthorrhiza Roxb as novel HNMT inhibitor compounds.
Inhibitory activity of Urena lobata leaf extract on alpha-amylase and alpha-glucosidase: in vitro and in silico approach
Authors: Purnomo Yudi , Makdasari Juliah , Fatahillah Faiqoh Inayah 
Published: July 2021

Abstract

Objectives
In food ingestion, alpha-glucosidase (α-glucosidase) and alpha-amylase (α-amylase) are enzymes that are responsible to convert a carbohydrate into glucose. Inhibition of both enzyme activities can prolong absorption of glucose in intestine and reduce post-prandial increase of blood glucose concentration, thus, it is beneficial for type-2 diabetes treatment. Traditionally, Urena lobata (U. lobata) has been used to manage diabetes, but the scientific proof of this claim remains scarce. Therefore, the objective of this study to examine the anti-diabetic potential of U. lobata leaf extract through inhibition of α-amylase and α-glucosidase.

Methods

U. lobata leaf extract was obtained through extraction process using ethanol and the chemical compounds in the extract were analyzed by liquid chromatography–mass spectra (LC–MS). The inhibitory activity of U. lobata on α-glucosidase and α-amylase was evaluated by in silico using docking server, whereas in vitro enzymatic assays were using para-nitrophenyl-α-d-glucopyranoside (α-NPG) and starch as substrates. The data were presented as mean ± SD and the IC50 value was calculated using SPSS.

Results

U. lobata leaf extract showed inhibitory activity on α-glucosidase and α-amylase with the IC50 value was 43.73 and 83.73 μg/mL, respectively, meanwhile, acarbose as standard has IC50 value at 1.14 and 0.08 μg/mL. Molecular docking study indicated β-sitosterol and stigmasterol from U. lobata extract have a huge inhibitory activity both on α-amylase and α-glucosidase based on inhibition constant (Ki) value.

Conclusions
Ethanolic extract of U. lobata showed inhibition activity on α-glucosidase stronger than on α-amylase as antidiabetic.
Genetic profile mutation rpoB in clinical isolate of rifampicin-resistant Staphylococcus aureus
Authors: Zulfiana Risa , Suharjono  , Kuntaman  
Published: July 2021

Abstract

Objectives

Staphylococcus aureus is one of the bacteria which causes nosocomial infection. Methicillin-Resistant Staphylococcus Aureus eradication using antibiotics combined with rifampicin has shown good results, whereas, adjuvant rifampicin has long been hypothesized to improve the outcome of S. aureus infection treatment. Resistant-rifampicin S. aureus mutates in rpoB gene at some codons. This study was conducted to identify the mutation of rpoB gene in S. aureus which was resistant toward rifampicin.

Methods
In this study, isolates collected in the Microbiology Laboratory of Dr. Seotomo Surabaya Hospital during May–September 2019. Then, the dilution method was carried out to determine the minimum inhibition concentration for resistant-rifampicin and dilution to determine the inhibition zone diameter. After that, DNA extraction was carried out from rifampicin-susceptible isolates as a control and resistant-rifampicin isolates followed by identification of rpoB gene mutations by Polymerase Chain Reaction (PCR) and sequencing.

Results
There were nine isolates studied. They were four resistant-rifampicin isolates and four susceptible-rifampicin isolates. In four rifampicin-resistant isolates, the most frequent mutations that occurred was His-481 codon (75%) followed by the Ile-527 codon (25%). Rifampicin-susceptible isolates mutated in Pro-475 and Asn-474 codons. One rifampicin-resistant isolate had two mutations in codons Ile-527 and Asn-474.

Conclusions
The type of mutation that causes the most rifampicin resistance was a missense mutation. The susceptible-rifampicin isolate experienced silent mutations. There was a relation between the type of missense mutation of rpoB gene and rifampin resistance.