bluuebunny/crossref_metadata_2025_split huggingface dataset:
Electrophysiological engineering of heart-derived cells with calcium-dependent potassium channels improves cell therapy efficacy for cardioprotection
Authors: Vigneault Patrick , Parent Sandrine , Kanda Pushpinder , Michie Connor , Davis Darryl R. , Nattel Stanley 
Published: August 2021

AbstractWe have shown that calcium-activated potassium (KCa)-channels regulate fundamental progenitor-cell functions, including proliferation, but their contribution to cell-therapy effectiveness is unknown. Here, we test the participation of KCa-channels in human heart explant-derived cell (EDC) physiology and therapeutic potential. TRAM34-sensitive KCa3.1-channels, encoded by theKCNN4gene, are exclusively expressed in therapeutically bioactive EDC subfractions and maintain a strongly polarized resting potential; whereas therapeutically inert EDCs lack KCa3.1 channels and exhibit depolarized resting potentials. Somatic gene transfer ofKCNN4results in membrane hyperpolarization and increases intracellular [Ca2+], which boosts cell-proliferation and the production of pro-healing cytokines/nanoparticles. Intramyocardial injection of EDCs afterKCNN4-gene overexpression markedly increases the salutary effects of EDCs on cardiac function, viable myocardium and peri-infarct neovascularization in a well-established murine model of ischemic cardiomyopathy. Thus, electrophysiological engineering provides a potentially valuable strategy to improve the therapeutic value of progenitor cells for cardioprotection and possibly other indications.
Excessive ammonium assimilation by plastidic glutamine synthetase causes ammonium toxicity in Arabidopsis thaliana
Authors: Hachiya Takushi , Inaba Jun , Wakazaki Mayumi , Sato Mayuko , Toyooka Kiminori , Miyagi Atsuko , Kawai-Yamada Maki , Sugiura Daisuke , Nakagawa Tsuyoshi , Kiba Takatoshi , Gojon Alain , Sakakibara Hitoshi 
Published: August 2021

AbstractPlants use nitrate, ammonium, and organic nitrogen in the soil as nitrogen sources. Since the elevated CO2 environment predicted for the near future will reduce nitrate utilization by C3 species, ammonium is attracting great interest. However, abundant ammonium nutrition impairs growth, i.e., ammonium toxicity, the primary cause of which remains to be determined. Here, we show that ammonium assimilation by GLUTAMINE SYNTHETASE 2 (GLN2) localized in the plastid rather than ammonium accumulation is a primary cause for toxicity, which challenges the textbook knowledge. With exposure to toxic levels of ammonium, the shoot GLN2 reaction produced an abundance of protons within cells, thereby elevating shoot acidity and stimulating expression of acidic stress-responsive genes. Application of an alkaline ammonia solution to the ammonium medium efficiently alleviated the ammonium toxicity with a concomitant reduction in shoot acidity. Consequently, we conclude that a primary cause of ammonium toxicity is acidic stress.
Structural basis for ALK2/BMPR2 receptor complex signaling through kinase domain oligomerization
Authors: Agnew Christopher , Ayaz Pelin , Kashima Risa , Loving Hanna S. , Ghatpande Prajakta , Kung Jennifer E. , Underbakke Eric S. , Shan Yibing , Shaw David E. , Hata Akiko , Jura Natalia 
Published: August 2021

AbstractUpon ligand binding, bone morphogenetic protein (BMP) receptors form active tetrameric complexes, comprised of two type I and two type II receptors, which then transmit signals to SMAD proteins. The link between receptor tetramerization and the mechanism of kinase activation, however, has not been elucidated. Here, using hydrogen deuterium exchange mass spectrometry (HDX-MS), small angle X-ray scattering (SAXS) and molecular dynamics (MD) simulations, combined with analysis of SMAD signaling, we show that the kinase domain of the type I receptor ALK2 and type II receptor BMPR2 form a heterodimeric complex via their C-terminal lobes. Formation of this dimer is essential for ligand-induced receptor signaling and is targeted by mutations in BMPR2 in patients with pulmonary arterial hypertension (PAH). We further show that the type I/type II kinase domain heterodimer serves as the scaffold for assembly of the active tetrameric receptor complexes to enable phosphorylation of the GS domain and activation of SMADs.
The effect of particle shape on the deformation and stress reduction of a gravel soil due to wetting
Authors: Mahinroosta Reza , Oshtaghi Vahid 
Published: August 2021

AbstractThis paper investigates the effect of particle shape on the stress reduction and collapse deformation of gravelly soil using a medium-scale direct shear test apparatus under different relative densities, normal stress, and shear stress levels. A new method based on the Micro-Deval test was introduced to produce sub-angular particles from angular particles. Therefore, two series of soil specimens were obtained with the same rock origin, particle size distribution, and relative density but different particle shapes. In addition to traditional direct shear tests on dry and wet specimens, a specific test procedure was applied to explore the stress reduction and collapse of soil specimens due to wetting. The results of the tests, including shear stress–shear displacement and vertical displacement-shear displacement, were compared. The results showed that the stress reduction and settlement due to wetting increased with vertical and shear stress levels in both types of particle shapes, with higher values in angular particle shapes. The particle breakage of the soil specimens was also studied quantitatively using the change in the particle size distribution before and after the test. It was shown that the wetting of the samples had more impact on the particle breakage in angular gravel than sub-angular gravel, which increased linearly with the normal stress.
Inaccuracy of a non-invasive estimation of pulmonary vascular resistance assessed by cardiovascular magnetic resonance in heart failure patients
Authors: Rumiz Eva , Cubillos Andrés , Vilar Juan Vicente , García Pilar , Berenguer Alberto , Fácila Lorenzo , Valero Ernesto , Vidal Verónica , Morell Salvador , Nuñez Julio 
Published: August 2021

AbstractPulmonary vascular resistance (PVR) is a marker of pulmonary vascular remodeling. A non-invasive model assessed by cardiovascular magnetic resonance (CMR) has been proposed to estimate PVR. However, its accuracy has not yet been evaluated in patients with heart failure. We prospectively included 108 patients admitted with acute heart failure (AHF), in whom a right heart catheterization (RHC) and CMR were performed at the same day. PVR was estimated by CMR applying the model: PVR = 19.38 − [4.62 × Ln pulmonary artery average velocity (in cm/s)] − [0.08 × right ventricle ejection fraction (in %)], and by RHC using standard formulae. The median age of the cohort was 67 years (interquartile range 58–73), and 34% were females. The median of PVR assessed by RHC and CMR were 2.2 WU (1.5–4) and 5 WU (3.4–7), respectively. We found a weak correlation between invasive PVR and PVR assessed by CMR (Spearman r = 0.21, p = 0.02). The area under the ROC curve for PVR assessed by CMR to detect PVR ≥ 3 WU was 0.57, 95% confidence interval (CI): 0.47–0.68. In patients with AHF, the non-invasive estimation of PVR using CMR shows poor accuracy, as well as a limited capacity to discriminate increased PVR values.
Mathematical analysis of a measles transmission dynamics model in Bangladesh with double dose vaccination
Authors: Kuddus Md Abdul , Mohiuddin M. , Rahman Azizur 
Published: August 2021

AbstractAlthough the availability of the measles vaccine, it is still epidemic in many countries globally, including Bangladesh. Eradication of measles needs to keep the basic reproduction number less than one $$(\mathrm{i}.\mathrm{e}. \, \, {\mathrm{R}}_{0}<1)$$

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.

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. This paper investigates a modified (SVEIR) measles compartmental model with double dose vaccination in Bangladesh to simulate the measles prevalence. We perform a dynamical analysis of the resulting system and find that the model contains two equilibrium points: a disease-free equilibrium and an endemic equilibrium. The disease will be died out if the basic reproduction number is less than one $$(\mathrm{i}.\mathrm{e}. \, \, {\mathrm{ R}}_{0}<1)$$

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, and if greater than one $$(\mathrm{i}.\mathrm{e}. \, \, {\mathrm{R}}_{0}>1)$$

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epidemic occurs. While using the Routh-Hurwitz criteria, the equilibria are found to be locally asymptotically stable under the former condition on $${\mathrm{R}}_{0}$$

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. The partial rank correlation coefficients (PRCCs), a global sensitivity analysis method is used to compute $${\mathrm{R}}_{0}$$

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and measles prevalence $$\left({\mathrm{I}}^{*}\right)$$

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with respect to the estimated and fitted model parameters. We found that the transmission rate $$(\upbeta )$$

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had the most significant influence on measles prevalence. Numerical simulations were carried out to commissions our analytical outcomes. These findings show that how progression rate, transmission rate and double dose vaccination rate affect the dynamics of measles prevalence. The information that we generate from this study may help government and public health professionals in making strategies to deal with the omissions of a measles outbreak and thus control and prevent an epidemic in Bangladesh.
Cardioprotective effects of early intervention with sacubitril/valsartan on pressure overloaded rat hearts
Authors: Li Xiaofei , Braza Julie , Mende Ulrike , Choudhary Gaurav , Zhang Peng 
Published: August 2021

AbstractLeft ventricular remodeling due to pressure overload is associated with poor prognosis. Sacubitril/valsartan is the first-in-class Angiotensin Receptor Neprilysin Inhibitor and has been demonstrated to have superior beneficial effects in the settings of heart failure. The aim of this study was to determine whether sacubitril/valsartan has cardioprotective effect in the early intervention of pressure overloaded hearts and whether it is superior to valsartan alone. We induced persistent left ventricular pressure overload in rats by ascending aortic constriction surgery and orally administrated sacubitril/valsartan, valsartan, or vehicle one week post operation for 10 weeks. We also determined the effects of sacubitril/valsartan over valsartan on adult ventricular myocytes and fibroblasts that were isolated from healthy rats and treated in culture. We found that early intervention with sacubitril/valsartan is superior to valsartan in reducing pressure overload-induced ventricular fibrosis and in reducing angiotensin II-induced adult ventricular fibroblast activation. While neither sacubitril/valsartan nor valsartan changes cardiac hypertrophy development, early intervention with sacubitril/valsartan protects ventricular myocytes from mitochondrial dysfunction and is superior to valsartan in reducing mitochondrial oxidative stress in response to persistent left ventricular pressure overload. In conclusion, our findings demonstrate that sacubitril/valsartan has a superior cardioprotective effect over valsartan in the early intervention of pressure overloaded hearts, which is independent of the reduction of left ventricular afterload. Our study provides evidence in support of potential benefits of the use of sacubitril/valsartan in patients with resistant hypertension or in patients with severe aortic stenosis.
Eight-year longitudinal study of whole blood gene expression profiles in individuals undergoing long-term medical follow-up
Authors: Sakai Yoshio , Nasti Alessandro , Takeshita Yumie , Okumura Miki , Kitajima Shinji , Honda Masao , Wada Takashi , Nakamura Seiji , Takamura Toshinari , Tamura Takuro , Matsubara Kenichi , Kaneko Shuichi 
Published: August 2021

AbstractBlood circulates throughout the body via the peripheral tissues, contributes to host homeostasis and maintains normal physiological functions, in addition to responding to lesions. Previously, we revealed that gene expression analysis of peripheral blood cells is a useful approach for assessing diseases such as diabetes mellitus and cancer because the altered gene expression profiles of peripheral blood cells can reflect the presence and state of diseases. However, no chronological assessment of whole gene expression profiles has been conducted. In the present study, we collected whole blood RNA from 61 individuals (average age at registration, 50 years) every 4 years for 8 years and analyzed gene expression profiles using a complementary DNA microarray to examine whether these profiles were stable or changed over time. We found that the genes with very stable expression were related mostly to immune system pathways, including antigen cell presentation and interferon-related signaling. Genes whose expression was altered over the 8-year study period were principally involved in cellular machinery pathways, including development, signal transduction, cell cycle, apoptosis, and survival. Thus, this chronological examination study showed that the gene expression profiles of whole blood can reveal unmanifested physiological changes.
Molecular epidemiology and HIV-1 variant evolution in Poland between 2015 and 2019
Authors: Serwin Karol , Urbańska Anna , Scheibe Kaja , Witak-Jędra Magdalena , Jankowska Maria , Hlebowicz Maria , Bociąga-Jasik Monika , Kalinowska-Nowak Anna , Biała Martyna , Ciepłucha Hubert , Łojewski Władysław , Olczak Anita , Jabłonowska Elżbieta , Kowalczuk-Kot Aldona , Rozpłochowski Błażej , Witor Adam , Parczewski Miłosz 
Published: August 2021

AbstractThe occurrence of HIV-1 subtypes differs worldwide and within Europe, with non-B variants mainly found across different exposure groups. In this study, we investigated the distribution and temporal trends in HIV-1 subtype variability across Poland between 2015 and 2019. Sequences of the pol gene fragment from 2518 individuals were used for the analysis of subtype prevalence. Subtype B was dominant (n = 2163, 85.90%). The proportion of subtype B-infected individuals decreased significantly, from 89.3% in 2015 to 80.3% in 2019. This was related to the increasing number of subtype A infections. In 355 (14.10%) sequences, non-B variants were identified. In 65 (2.58%) samples, recombinant forms (RFs) were noted. Unique recombinant forms (URFs) were found in 30 (1.19%) sequences. Three A/B recombinant clusters were identified of which two were A6/B mosaic viruses not previously described. Non-B clades were significantly more common among females (n = 81, 22.8%, p = 0.001) and heterosexually infected individuals (n = 45, 32.4%, p = 0.0031). The predominance of subtype B is evident, but the variability of HIV-1 in Poland is notable. Almost half of RFs (n = 65, 2.58%) was comprised of URFs (n = 30, 1.19%); thus those forms were common in the analyzed population. Hence, molecular surveillance of identified variants ensures recognition of HIV-1 evolution in Poland.
Comparative genome analysis of Bacillus thuringiensis strain HD521 and HS18-1
Authors: Sun Hongwei , Xiang Xing , Li Qiao , Lin Hui , Wang Xiaolin , Sun Jie , Luo Long , Zheng Aiping 
Published: August 2021

AbstractBacillus thuringiensis (Bt) is an important biological insecticide used to management of different agricultural pests by producing toxic parasporal crystals proteins. Strain HD521 has an antagonistic effect against Rhizoctonia solani AG1IA, the causal agent of rice sheath blight. This strain with three cry7 genes can the formation of bipyramidal parasporal crystals (BPCs). BPCs are used for insecticidal activities against Henosepilachna vigintioctomaculata larva (Coleoptera). Strain HS18-1 contains different types of BPCs encoding genes and has effective toxicity for Lepidoptera and Diptera insects. Here we report the whole genome sequencing and assembly of HD521 and HS18-1 strains and analyzed the genome constitution covering virulence factors, types of plasmid, insertion sequences, and prophage sequences. The results showed that the genome of strain HD521 contains a circular chromosome and six circular plasmids, encoding eight types of virulence protein factors [Immune Inhibitor A, Hemolytic Enterotoxin, S-layer protein, Phospholipase C, Zwittermicin A-resistance protein, Metalloprotease, Chitinase, and N-acyl homoserine lactonase (AiiA)], four families of insertion sequence, and comprises six pro-phage sequences. The genome of strain HS18-1 contains one circular chromosome and nine circular plasmids, encoding five types of virulence protein factors [Hemolytic Enterotoxin, S-layer protein, Phospholipase C, Chitinase, and N-acyl homoserine lactonase (AiiA)] and four families of insertion sequence, and comprises of three pro-phage sequences. The obtained results will contribute to deeply understand the B. thuringiensis strain HD521 and HS18-1 at the genomic level.
Evaluation of the association between periodontitis and risk of Parkinson’s disease: a nationwide retrospective cohort study
Authors: Jeong Eunkyung , Park Jun-Beom , Park Yong-Gyu 
Published: August 2021

AbstractThe objective of this study was to examine the association between periodontitis and risk of incident Parkinson’s disease using large-scale cohort data on the entire population of South Korea. Health checkup data from 6,856,180 participants aged 40 and older were provided by the National Health Insurance Service of South Korea between January 1, 2009, and December 31, 2009, and the data were followed until December 31, 2017. The hazard ratio (HR) of Parkinson’s disease and 95% confidence interval (CI) were estimated using a Cox proportional hazards model adjusted for potential confounders. The incidence probability of Parkinson’s disease was positively correlated with the presence of periodontitis. The HR of Parkinson’s disease for the participants without the need of further dentist visits was 0.96 (95% CI 0.921–1.002); the HR of Parkinson’s disease increased to 1.142 (95% CI 1.094–1.193) for the individuals who needed further dentist visits. Compared to individuals without periodontitis and without metabolic syndrome, the HR of incident Parkinson’s disease gradually increased for individuals with periodontitis, with metabolic syndrome, and with both periodontitis and metabolic syndrome. People with periodontitis and metabolic syndrome had the highest HR of incident Parkinson’s disease, at 1.167 (95% CI 1.118–1.219). In conclusion, a weak association between periodontitis and Parkinson’s disease was suggested after adjusting for confounding factors from the population-based large-scale cohort of the entire South Korean population.
Optimization of solid waste collection using RSM approach, and strategies delivering sustainable development goals (SDG’s) in Jeddah, Saudi Arabia
Authors: Radwan Neyara , Khan Nadeem A. , Elmanfaloty Rania Abdou Gaber 
Published: August 2021

AbstractThe rapid industrial development, high population growth, and rapid urbanization of Saudi Arabia have led to increased pollution and waste levels. Every day, solid waste disposal for governments and local authorities becomes a significant challenge. Saudi Arabia produces over 15 million tonnes of solid waste annually, with a population of around 29 million. The waste production per person is estimated at between 1.5 and 1.8 kg per day per person. About 75% of the population in urban areas is concentrated, making it imperative that government steps are taken to boost the country's waste recycling and management scenario. The production of solid waste in Riyadh, Jeddah, and Dammam, three of the largest cities, exceeds seven million tonnes annually, which shows the enormity of the civic body problem. During this study, the design Expert software was involved in the optimization of process parameters during the collection of municipal solid waste (MSW) from Jeddah city. The use of design experiments and numerical optimization is quite effective in optimizing the different process parameters on the overall cost. Saudi Arabia has a critical need for a resilient waste system and agile waste management system to control its municipal solid waste quickly and environmentally friendly for achieve Saudi Vision 2030. For this study design of experiment, software was employed to optimize the cost per trip, thereby considering process parameters. It is therefore essential to examine the existing practices and future opportunities for solid waste collection, storage, and disposal. This study considered that MSW generated in Saudi Arabia which is having great potential to be converted into wealth. Hence, considering the current environment situation, energy prospective and future management strategies for MSW have also been reviewed.
The functional brain favours segregated modular connectivity at old age unless affected by neurodegeneration
Authors: Chen Xue , Necus Joe , Peraza Luis R. , Mehraram Ramtin , Wang Yanjiang , O’Brien John T. , Blamire Andrew , Kaiser Marcus , Taylor John-Paul 
Published: August 2021

AbstractBrain’s modular connectivity gives this organ resilience and adaptability. The ageing process alters the organised modularity of the brain and these changes are further accentuated by neurodegeneration, leading to disorganisation. To understand this further, we analysed modular variability—heterogeneity of modules—and modular dissociation—detachment from segregated connectivity—in two ageing cohorts and a mixed cohort of neurodegenerative diseases. Our results revealed that the brain follows a universal pattern of high modular variability in metacognitive brain regions: the association cortices. The brain in ageing moves towards a segregated modular structure despite presenting with increased modular heterogeneity—modules in older adults are not only segregated, but their shape and size are more variable than in young adults. In the presence of neurodegeneration, the brain maintains its segregated connectivity globally but not locally, and this is particularly visible in dementia with Lewy bodies and Parkinson’s disease dementia; overall, the modular brain shows patterns of differentiated pathology.
Synergetic effect-triggered performance promotion of Sr3−xBaxP5N10Cl:Eu2+phosphors
Authors: Hsueh Hsi-Ping , Fang Mu-Huai , Vasudevan Thangaraji , Huang Wen-Tse , Majewska Natalia , Lazarowska Agata , Mahlik Sebastian , Sheu Hwo-Shuenn , Lee Jyh-Fu , Liu Ru-Shi 
Published: August 2021

A series of zeolitic nitridophosphate phosphors Sr2.91−xBaxP5N10Cl:0.09Eu2+ was synthesized using a hot isostatic press.
The reproductive tract microbiota in pregnancy
Authors: Grewal Karen , MacIntyre David A. , Bennett Phillip R. 
Published: August 2021

Abstract
The reproductive tract microbiota plays a crucial role in maintenance of normal pregnancy and influences reproductive outcomes. Microbe–host interactions in pregnancy remain poorly understood and their role in shaping immune modulation is still being uncovered. In this review, we describe the composition of vaginal microbial communities in the reproductive tract and their association with reproductive outcomes. We also consider strategies for manipulating microbiota composition by using live biotherapeutics, selective eradication of pathogenic bacteria with antibiotics and vaginal microbiota transplantation. Finally, future developments in this field and the need for mechanistic studies to explore the functional significance of reproductive tract microbial communities are highlighted.