bluuebunny/crossref_metadata_2025_split huggingface dataset:
Automated Light-Induced Synthesis of 89Zr-Radiolabeled Antibodies for Immuno-Positron Emission Tomography
Authors: Klingler Simon , Holland Jason 
Published: October 2021

Abstract
Clinical production of 89Zr-radiolabeled antibodies (89Zr-mAbs) for positron emission tomography (PET) imaging relies on the pre-conjugation of desferrioxamine B (DFO) to the purified protein, followed by isolation and characterization of the functionalized intermediate, and then manual radiosynthesis. Although highly successful, this route exposes radiochemists to a potentially large radiation dose and entails several technological and economic hurdles that limit access of 89Zr-mAbs to just a specialist few Nuclear Medicine facilities worldwide. Here, we introduce a fully automated synthesis box that can produce individual doses of 89Zr-mAbs formulated in sterile solution in <25 min starting from [89Zr(C2O4)4]4– (89Zr-oxalate), our Good Laboratory Practice-compliant photoactivatable desferrioxamine-based chelate (DFO-PEG3-ArN3), and clinical-grade antibodies without the need for pre-purification of protein. The automated steps include neutralization of the 89Zr-oxalate stock, chelate radiolabeling, and light-induced protein conjugation, followed by 89Zr-mAb purification, formulation, and sterile filtration. As proof-of-principle, 89ZrDFO-PEG3-azepin-trastuzumab was synthesized directly from Herceptin in <25 min with an overall decay-corrected radiochemical yield of 20.1±2.4% (n=3), a radiochemical purity >99%, and chemical purity >99%. The synthesis unit can also produce 89Zr-mAbs via the conventional radiolabeling routes from pre-functionalized DFO-mAbs that are currently used in the clinic. This automated method will improve access to state-of-the-art 89Zr-mAbs at the many Nuclear Medicine and research institutions that require automated devices for radiotracer production.
Is Aryl Hydrocarbon Receptor Antagonism After Ischemia Effective in Alleviating Hepatic Ischemia-Reperfusion Injury in Rats?
Authors: Kwon Jae-Im , Heo Hwon , Chae Yeon Ji , Min Joongkee , Lee Do-Wan , Kim Sang Tae , Choi Monica Young , Sung Yu Sub , Kim Kyung Won , Choi Yoonseok , Woo Dong Cheol , Woo Chul-Woong 
Published: October 2021

Abstract
Aryl hydrocarbon receptors (AhRs) have been reported to be important mediators of ischemic injury in the brain. Furthermore, the pharmacological inhibition of AhR activation after ischemia has been shown to attenuate cerebral ischemia-reperfusion (IR) injury. Here, we investigated whether AhR antagonist administration after ischemia was also effective in ameliorating hepatic IR injury. A 70% partial hepatic IR (45-minute ischemia and 24-hour reperfusion) injury was induced in rats. We administered 6,2',4'-trimethoxyflavone (TMF, 5 mg/kg) intraperitoneally 10 minutes after ischemia. Hepatic IR injury was observed using serum, magnetic resonance imaging-based liver function indices, and liver samples. TMF-treated rats showed significantly lower relative enhancement (RE) values and serum alanine aminotransferase (ALT) and aspartate aminotransferase levels than did untreated rats at three hours after reperfusion. After 24 hours of reperfusion, TMF-treated rats had significantly lower RE values, ΔT1 values, serum ALT levels, and necrotic area percentage than did untreated rats. The expression of the apoptosis-related proteins, Bax and cleaved caspase-3, was significantly lower in TMF-treated rats than in untreated rats. This study demonstrated that inhibition of AhR activation after ischemia was effective in ameliorating IR-induced liver injury in rats.
Aberrant Resting-State Regional Activity in Patients with Postpartum Depression
Authors: Zhang Shufen , Li Bo , Liu Kai , hou xiaoming , Zhang Ping 
Published: October 2021

Abstract
The aim of this study is to investigate the alterations of individual local connectivity by regional homogeneity (ReHo) in patients with postpartum depression (PPD) during resting state, and their potential correlations with clinical severity. Thirty patients with PPD and twenty-nine matched healthy postpartum women within 4 weeks after delivery were recruited and performed resting-state functional magnetic resonance imaging (fMRI) scans. The ReHo value was computed as Kendall’s coefficient of concordance (KCC) in the present study, and intergroup differences in the voxel manner were analyzed. Correlations between ReHo values and clinical variables were also analyzed. Compared with healthy postpartum women, patients with PPD exhibited significantly higher ReHo in left precuneus and right hippocampus. ReHo was significantly lower in left dorsolateral prefrontal cortex (dlPFC) and right insula. Furthermore, ReHo values within the dlPFC negatively correlated with the Edinburgh postpartum depression scale (EPDS) score. The ROC curve analysis showed that the area under the curve (AUC) of the above four brain regions are all over 0.7. This study provided evidences of aberrant regional functional activity within brain regions involved in the maternal care network in PPD, and may contribute to the further understanding of neurobiology underlying PPD.
Multifunctional Carbonized Nanogels to Treat Lethal Acute Hepatopancreatic Necrosis Disease
Authors: Yen Shao-Chieh , Mao Ju-Yi , Lin Hung-Yun , Huang Huai-Ting , Harroun Scott G. , Nain Amit , Chang Huan-Tsung , Lin Han-You , Chen Li-Li , Huang Chih-Ching , Lin Han-Jia 
Published: October 2021

Abstract
Background: Shrimp aquaculture has suffered huge economic losses over the past decade due to the outbreak of acute hepatopancreatic necrosis disease (AHPND), which is mainly caused by the bacteria Vibrio parahaemolyticus (V. parahaemolyticus) with the virulence pVA1 plasmid, which encodes a secretory photorhabdus insect-related (Pir) toxin composed of PirA and PirB proteins. The Pir toxin mainly attacks the hepatopancreas, a major metabolic organ in shrimp, thereby causing necrosis and loss of function. The pandemic of antibiotic-resistant strains makes the impact worse. Methods: Mild pyrolysis of a mixture of polysaccharide dextran 70 and the crosslinker 1,8-diaminooctane at 180 ℃ for 3 h to form carbonized nanogels (DAO/DEX-CNGs) through controlled cross-linking and carbonization. The multifunctional therapeutic CNGs inherit nanogel-like structures and functional groups from their precursor molecules. Results: DAO/DEX-CNGs manifest broad-spectrum antibacterial activity against Vibrio parahaemolyticus responsible for AHPND and even multiple drug-resistant strains. The polymer-like structures and functional groups on graphitic-carbon within the CNGs exhibit multiple treatment effects, including disruption of bacterial membranes, elevating bacterial oxidative stress, and neuralization of PirAB toxins. The inhibition of Vibrio in the midgut of infected shrimp, protection of hepatopancreas tissue from Pir toxin, and suppressing overstimulation of the immune system in severe V. parahaemolyticus infection, revealing that CNGs can effectively guard shrimp from Vibrio invasion. Moreover, shrimps fed with DAO/DEX-CNGs were carefully examined, such as the expression of the immune-related genes, hepatopancreas biopsy, and intestinal microbiota. Few adverse effects on shrimps were observed. Conclusion: Our work proposes brand-new applications of multifunctional carbon-based nanomaterials as efficient anti-Vibrio agents in the aquatic industry that hold great potential as feed additives to reduce antibiotic overuse in aquaculture.
Characteristics and Clinical Significance of Lipid Metabolism in Patients with Gastrointestinal Stromal Tumor
Authors: Liu Xiaoling , Liu Bende , Hu Jun 
Published: October 2021

Abstract
Objective To investigate the characteristics and clinical significance of serum lipids in patients with gastrointestinal stromal tumor (GISTs).Methods The clinical and pathological data of 694 cases of GIST patients in Liyuan hospital and Union hospital from 2012 to 2016 were retrospectively analyzed. To compare the differences of blood lipid levels in patients with different risk levels.Results There was no significant correlation between preoperative lipid profile and some clinical features, including age, tumor frequency, CD117 or SMA positive or negative, and Ki67% < 5% or not (P ＞0.05). LDL-C, HDL-C and CHOL were significantly higher in women than men (P < 0.05). Compared to patients with tumor size over 5cm, TG, HDL-C and CHOL were significantly higher in those under 5cm (all P < 0.05). LDL-C, HDL-C and CHOL were higher in CD34 positive subjects than in negative ones. TG in the DOG-1 positive group was higher than that in negative one (P < 0.05). HDL-C was lower in S-100 positive subjects than in S-100 negative subjects (P < 0.05). Lipids indexes were correlated with the risk grade and tumor site of GISTs (P < 0.05). TG/HDL-C was significantly different in patients with GIST with different tumor sites, CD34 and DOG-1 positive or not (P < 0.05).Conclusion The clinical and pathological characteristics of the patients with GIST are closely related to the level of blood lipids. Blood lipids level can help judge benign or malignant of GIST to a certain extent.
The Economic Fitness of Nations in Value Added. 2000-2014
Authors: Cervantes-Martinez Rosario , Villaseñor-Becerra Jorge 
Published: October 2021

Abstract
In this paper, we explore the Economic Fitness (EF) indicator from the World Bank Database that measures nations' level of international competitiveness. At the same time, using input-output tables, we present our estimations of this new metric, replacing the revealed comparative advantages (RCA) from exports in gross value with an estimate of RCA from exports in domestic value-added (DVA). We find that between 1995 and 2015, there is a positive relationship between Economic Fitness and per capita GDP for low and middle-income countries. Besides, from 2000 to 2014, there has been a widening gap between global exports in gross value and exports in value-added; we also show that the estimations of the EF using the domestic value-added content of exports change significantly. Suggesting that, given the increasing levels of productive links at the international level, the competitiveness of nations is also conditioned by the way they participate in global production networks.
Non-intrusive Nonlinear Model Reduction via Machine Learning Approximations to Low-dimensional Operators
Authors: Bai Zhe , Peng Liqian 
Published: October 2021

Abstract
Although projection-based reduced-order models (ROMs) for parameterized nonlinear dynamical systems have demonstrated exciting results across a range of applications, their broad adoption has been limited by their intrusivity: implementing such a reduced-order model typically requires significant modifications to the underlying simulation code. To address this, we propose a method that enables traditionally intrusive reduced-order models to be accurately approximated in a non-intrusive manner. Specifically, the approach approximates the low-dimensional operators associated with projection-based reduced-order models (ROMs) using modern machine-learning regression techniques. The only requirement of the simulation code is the ability to export the velocity given the state and parameters; this functionality is used to train the approximated low-dimensional operators. In addition to enabling nonintrusivity, we demonstrate that the approach also leads to very low computational complexity, achieving up to $10^3\times$ in run time. We demonstrate the effectiveness of the proposed technique on two types of PDEs.
Curcumin and Zinc Co-Supplementation Along With a Loss-Weight Diet Can Improve Lipid Profiles in Subjects with Pre-Diabetes: A Multi-Arm, Parallel-Group, Randomized, Double-Blind Placebo-Controlled Phase 2 Clinical Trial
Authors: Karandish Majid , Mozaffari-khosravi Hassan , Mohammadi Seyed Mohammad , Cheraghian Bahman , Azhdari Maryam 
Published: October 2021

Abstract
Background: Diabetes is one of the major public health concerns. Pre-diabetes can increase the risk of developing some non-communicable diseases such as type 2 diabetes. Given the increasing trend of pre-diabetes, it is critical to control it and prevent its complications. Curcumin is a major bioactive component of turmeric. Zinc is an antioxidant nutrient. The present trial aimed to evaluate the effect of curcumin and zinc co-supplementation along with a loss-weight diet on serum lipid profiles in overweight or obese patients with pre-diabetes.Methods: Eighty-four participants were randomized to four groups (curcumin (500 mg/day), zinc (30 mg/day), “curcumin & zinc”, and placebo) for 90 days. Serum total cholesterol (TC), low-density lipoprotein (LDL), high-density lipoprotein (HDL), triglycerides (TG), non-HDL, HDL/LDL ratio, weight, BMI, waist circumstance (WC), hip circumstance (HC), physical activity (PA) and dietary intake were determined pre and post-intervention. This study will be conducted at Yazd Diabetes Research Clinic, Shahid Sadoughi University of Medical Sciences.Results: Totally, 82 participants were included in the final analysis. After the adjusted PA effect, changes in serum TG (adjusted p = 0.001), LDL (adjusted p = 0.035), non-HDL (adjusted p =0.003), HDL/LDL ratio (adjusted p =0.002), and HDL (adjusted p < 0.0001) revealed a significant difference between the groups. However, the changes in weight (adjusted p = 0.004) and BMI (adjusted p = 0.006) were significant but the changes in dietary intake, PA, WC, and HC were non-significant (adjusted p ≥ 0.05). Despite that there was a significant difference for post-intervention HDL levels (adjusted p = 0.016), other lipid profiles showed no significant difference (adjusted p ≥ 0.05).Conclusion: The beneficial effects of “curcumin & zinc” co-supplementation was reported for the changes of some lipid profiles (TG, LDL, HDL, non-HDL, and HDL to LDL ratio), BMI, and weight with no positive effects on TC, dietary intake, PA, WC, and HC. Therefore, it may play a potential role in the prevention of macro and microvascular complications.Trial registration: The project is a registered clinical trial (Registration number: IRCT20190902044671N1, Iranian Registry of Clinical Trials (IRCT), registered October 11, 2019.
WITHDRAWN: The Two Mono-Carbonyl Curcumin Analogs, PGV-1 and CCA-1.1: The Chemopreventive Activity Against DMH-Induced Colorectal Cancer Rat and Proteins Target Candidate Involved
Authors: Wulandari Febri , Ikawati Muthi' , Kirihata Mitsunori , Widyarini Sitarina , Kato Jun-Ya , Meiyanto Edy 
Published: October 2021

Abstract
Pentagamavunone-1 and its newest derivatives, CCA-1.1, possess an outstanding cytotoxic activity against several cancer cell lines, especially colorectal cancer. We are continuing to explore the anti-colorectal cancer properties of PGV-1 and CCA-1.1 against DMH-induced colorectal cancer rats and expound the potential protein target in colorectal adenocarcinoma. We utilized DMH 60 mg/kg (subcutaneous injection once a week for 16 weeks) to induce colorectal cancer. PGV-1 and CCA-1.1 at 10 and 20 mg/kg (orally twice a week for 16 weeks) were co-administered with DMH. The WBC count increased in a single DMH group and was countered by co-administration of PGV-1 and CCA-1.1, but no significant differences in RBC. Single DMH treatment for 16 weeks resulted in 80% adenocarcinoma. In contrast, co-administration with PGV-1 and CCA-1.1 suppressed most of the carcinogenic characteristics and symptoms of the pre-malignancy condition. Furthermore, bioinformatics analysis showed that CCA-1.1 has more specific targets than PGV-1, including CDK1, CDK2, MMP3, MMP14, and CYP3A4, which regulate the cell cycle arrest, cancer cell migration, and xenobiotic metabolism, respectively. Interestingly, CCA-1.1 targets CYP3A4, which possibly interrupts DMH metabolism and prevents the initiation of DMH-colorectal carcinogenesis. In conclusion, CCA-1.1 performed better chemopreventive effects against DMH colorectal cancer and might replace PGV-1 to be promoted as a more effective anti-colorectal cancer agent.
The Traditional Medicine Practitioner’s Concept of Cancer, Herbal medicine use and the Patients Perceived benefits (Clinical outcomes) in Selected Districts of Central Uganda: An Exploratory Study Protocol
Authors:   John Baptist Asiimwe1, Nagendrappa Prakash B. , Atukunda Esther C. , Nambozi Grace , Tolo Casim U. , Ogwang  , Sarki Ahmed M. , Kamatenesi Maud M. 
Published: October 2021

Abstract

Background: In sub-Saharan Africa, herbal medicine for cancer is dispensed by traditional medicine practitioners (TMPs) to a large extent, who may not have adequate knowledge about the disease and this has been found to contribute to the late presentation of cancer patients to clinics and hospitals leading to poor patient outcomes.Aim: This study will investigate the traditional medicine practitioner's concept of cancer, its care, herbal medicine use, and the patients' perceived benefits (clinical outcomes) in selected districts of Central Uganda.Methods: This study will use a convergent parallel mixed-method design. TMPs claiming to treat cancer, with at least 10 years of experience will be recruited and interviewed about their understanding of cancer as a disease (grounded theory design) and the herbs used in cancer treatment (ethnobotanical survey). We will also conduct a prospective observational study to obtain the data on the perceived clinical outcomes of using herbal medicine. Adult patients (>18years) with a medical diagnosis of cancer signposted by TMPs will be recruited and followed up at baseline, 3, and 6 months. Study outcomes will include a change in the quality of life score, cancer symptoms score, perceived benefits score, and adverse reactions. The researcher will analyze qualitative data using Open, axial, selective coding techniques. We will analyze the quantitative data using descriptive statistics and results presented in frequencies, percentages and measures of central tendency (mean) where applicable. Additional analyzes such as bivariate (paired t-test and one-way repeated measures ANOVA), and multivariate (linear mixed effects model) analysis for the repeated measures data shall be undertaken to analyze the prospective observational data. P- Values of <0.05 will be considered statistically significant.Discussion: This study will aid researchers in further understanding the TMPs' conceptualization of cancer, how that links to the treatment dispensed to their patients, and ultimately the patient-perceived outcomes.
Building vibration monitoring based on digital optical cameras
Authors: Wang Wei , Shao Yu 
Published: October 2021

The complex and process-critical conditions of machineries are needed to be continuously monitored in order for incipient breakdown to detect and ensure its high performance and operating safety. The change in the resonant frequency is focused by the spectrum analysis which is caused by the structural degradation and for health monitoring, useful information is produced. A test signal is required by the spectral analysis for health monitoring of structural condition. A test signal is the easiest way for structural spectral characteristics measurement but during every measurement, a structure vibration is impractical. Several techniques are presented for the condition of a machine depending on the application monitoring. The structure health would be able to be monitored if structural vibrations are utilized for spectral characteristics measurement due to wind and ground motion. The vision-based monitoring with the sources of errors are detailed and presented in this paper. Different parameters are observed for the different signals, unify the scale and can also be used to signals errors as compared with different levels of amplitudes. The PSNR and Structural Similarity are analyzed, the PSNR value is obtained in the range of 40 dB to 50 dB and the SSIM values are nearby to the 1 for all the signals. The MAPE and MAE values obtained by the presented technique are less for all the signal samples. The presented method outperforms the existed technique by 3.21 % to 44.02 % in form of MAPE. The presented method is 3.21 % to 44.02 % better than the previously existed technique in form of MAPE. The percentage improvement of the presented method outperforms by 33.93 % and 42.43 % in terms of MAE.
Googling for Suicide–Content and Quality Analysis of Suicide-Related Websites: Thematic Analysis
Authors: Chen Wen , Boggero Andrea , Del Puente Giovanni , Olcese Martina , Prestia Davide , Jahrami Haitham , Chalghaf Nasr , Guelmami Noomen , Azaiez Fairouz , Bragazzi Nicola Luigi 
Published: October 2021

Background
Suicide represents a public health concern, imposing a dramatic burden. Prosuicide websites are “virtual pathways” facilitating a rise in suicidal behaviors, especially among socially isolated, susceptible individuals.

Objective
The aim of this study is to characterize suicide-related webpages in the Italian language.

Methods
The first 5 most commonly used search engines in Italy (ie, Bing, Virgilio, Yahoo, Google, and Libero) were mined using the term “suicidio” (Italian for suicide). For each search, the first 100 webpages were considered. Websites resulting from each search were collected and duplicates deleted so that unique webpages could be analyzed and rated with the HONcode instrument

Results
A total of 65 webpages were included: 12.5% (8/64) were antisuicide and 6.3% (4/64) explicitly prosuicide. The majority of the included websites had a mixed or neutral attitude toward suicide (52/64, 81.2%) and had informative content and purpose (39/64, 60.9%). Most webpages targeted adolescents as an age group (38/64, 59.4%), contained a reference to other psychiatric disorders or comorbidities (42/64, 65.6%), included medical/professional supervision or guidance (45/64, 70.3%), lacked figures or pictures related to suicide (41/64, 64.1%), and did not contain any access restraint (62/64, 96.9%). The major shortcoming to this study is the small sample size of webpages analyzed and the search limited to the keyword “suicide.”

Conclusions
Specialized mental health professionals should try to improve their presence online by providing high-quality material.
INTERVENTION OF CLINICAL PHARMACIST IN THE MANAGEMENT OF TYPE 2 DIABETES MELLITUS IN OUTPATIENTS
Authors: MATHEWS ANJU SARAH 
Published: October 2021

Objective: The purpose of the study was to analyze the effect of clinical pharmacist intervention on glycemic control based on fasting blood glucose and glycosylated blood glucose level.
Methods: A randomized prospective interventional study was conducted in the outpatient department of a tertiary care hospital. Patients suffering from diabetes for a least 2 y were selected for the study based on the inclusion and exclusion criteria. The control group was not given any special pharmacist care, while the interventional group had a face-to-face interview, counseling, and telephonic follow-up during the study period. Based on the baseline values and endpoint parametric values, the result of the study was analyzed.
Results: The study was analyzed based on the difference in the glycemic index, using HbA1c and FBS values. The basal values of HbA1c were similar for both groups (8.5%), but a marked reduction to 7.2% was observed in the interventional group. FBS values reduced from 208 mg/dl to 186 mg/dl in the intervention group, while in the usual care group, the reduction was from 211 mg/dl to 198 mg/dl.
Conclusion: The inclusion of clinical pharmacists in the healthcare team can offer a remarkable improvement in patient's condition by providing more support in the therapy.