bluuebunny/crossref_metadata_2025_split huggingface dataset:
Genome-Wide Identification and Expression Analysis of Flavonoid 3'-Hydroxylase Gene Family in Carthamus Tinctorius L.
Authors: Quốc Hoàng Việt Nguyễn , Jie Kong , Ahmad Naveed , Zhuoda Yang , Nan Wang , Xintong Ma , Yue Zhang Xin , Xiuming Liu , Na Yao , Libo Jin 
Published: June 2021

Abstract
ObjectiveThrough experiments and bioinformatic analysis clearly demonstrate considerable information about the genetic evolution of the flavonoid 3'-hydroxylase (F3'H) gene in Safflower and in plants.ResultsHere, we performed genome wide survey of safflower genome and identified a total of 22 CtF3'H enzyme encoding genes. Phylogenetic analyses revealed the classifications of these CtF3'Hs into nine subgroups demonstrating their evolutionary relationship. The distribution of the conserved protein motifs, and cis-regulatory units of CtF3'Hs indicated essential structure-to-function components leading to the final function of protein or its interactions. Furthermore, the results of digital expression analysis and the qRT-PCR pattern of 22 putative CtF3’H genes during different flowering stages suggested their requisite roles in safflower petal pigmentation. In addition, the fusions construct of plant expression vector pCAMBIA1302-GFP-CtF3’H5 in onion epidermal cells verified the subcellular localization of CtF3’H5 to the plasma membrane. Subsequently, the prokaryotic expression and western blot hybridization of CtF3’H5 resulted in a stable 50.3kD target protein. These results partly demonstrate the influence of F3'Hs on plants.ConclusionsIn this study, the results of digital expression and qRT-PCR analysis of 22 putative CtF3'H genes in different flowering stages indicate their essential role in safflower petal pigmentation. clearly demonstrates significant information on the genetic evolution of important enzyme-coding genes and will provide a pathway for future functional studies of F3'Hs in safflower as well as in plants.
Uncovering of the Association Between m5C Regulator-Mediated Methylation Modification Patterns and Tumor Microenvironment Infiltration Characteristics in Hepatocellular Carcinoma
Authors: Gu Xinyu , Zhou Haibo , Chu Qingfei , Zheng Qiuxian , Wang Jing , Zhu Haihong 
Published: June 2021

Abstract
Background: 5-Methylcytosine (m5C) plays essential roles in hepatocellular carcinoma (HCC), but the association between m5C regulation and immune cell infiltration in HCC has not yet been clarified.Methods: In this study, we analysed 371 patients with HCC from The Cancer Genome Atlas (TCGA) database, and the expression of 13 m5C regulators was investigated. Additionally, gene set variation analysis (GSVA), unsupervised clustering analysis, single-sample gene set enrichment analysis (ssGSEA), correlation analysis, and immunohistochemical (IHC) staining were performed.Results: Among the 371 patients, 41 had mutations in m5C regulators, the frequency of which was 11.26%. Then, we identified three m5C modification patterns that had obvious tumour microenvironment (TME) cell infiltration characteristics. Cluster-1 had an immune rejection phenotype; Cluster-2 had an immunoinflammatory phenotype; and Cluster-3 had an immune desert phenotype. In addition, we found that DNMT1 was highly expressed in tumour tissues compared with normal tissues in a tissue microarray (TMA) and that it was positively correlated with many TME-infiltrating immune cells. High expression of the m5C regulator DNMT1 was related to a poor prognosis in patients with HCC. Furthermore, we developed three Immu-clusters that were consistent with the immune characteristics of the m5C methylation modification patterns. We also discovered differences in the levels of immune cells and expression of chemokines and cytokines among the three Immu-clusters.Conclusions: Our work revealed the association between m5C modification and immune regulators in the TME. These findings also suggest that DNMT1 has great potential as a prognostic biomarker and therapeutic target for HCC.
Altered Proinflammatory Cytokines Distinguish Elderly with Major Depressive Disorder from Amnestic Mild Cognitive Impairment in a Chinese Population
Authors: Nie Jing , Fang Yuan , Qiu Qi , Li Yuan , Zhao Lu , Liu Xiang , Li Xia 
Published: June 2021

Abstract
Background: Research evidence has shown that late life depression(LLD)and amnestic mild cognitive impairment(aMCI)are both related to dementia and usually accompanied by inflammatory dysregulation, but few studies have compared the levels of chemokines and cytokines between these two disorders. In this cross-sectional study, we aimed at identifying differences in neuro-inflammation between patients with prodromal stage of AD (amnestic mild cognitive impairment, aMCI; Late life depression LLD) and the normal controls (NC).Additionally, the potential association between cognitive function and cytokines characteristics was evaluated.Methods: Using Luminex assays,we simultaneously measured 29 cytokines,including 6 CXC chemokine ligand(CXCL5,CXCL6,CXCL8,CXCL9,CXCL11,CXCL16),15 CC chemokine ligand(CCL1, CCL2,CCL3,CCL7, CCL8, CCL11, CCL13,CCL15,CCL17, CCL19,CCL20, CCL23, CCL24, CCL25, CCL27), IL-1β, MIP-2, IFN-γ, VCAM-1, IL-2, IL-4, TNF-α and IL-6 in plasma of subjects. Demographics, cognitive function and lifestyle factors were also collected. All subjects(n=69, 23 each group)were age- and gender-matched outpatients with LLD or aMCI without dementia(based on DSM-IV criteria)and normal controls(NC)in community. The Chinese versions of Montreal Cognitive Assessment(MoCA) and neuropsychological test battery (NTB) were used to evaluate the cognitive function in participants.Results: LLD patients showed remarkably higher level of IL-6 than the aMCI and NC groups. We also observed five CC chemokines (CCL2,CCL17,CCL15, CCL13and CCL5) and four CXCs (CXCL5,CXCL6,CXCL16 and CXCL11) were decreased in patients with LLD. In addition, CXCL5 and VCAM-1 were correlated with age and clinical characteristic with gender difference. Importantly, using two parameters(IL-6 and CCL13) could effectively distinguish LLD from aMCI with an area under curve of 0.809(95% CI 0.68-0.93).Conclusion: Under conditions of neuro-inflammation, distinct dysregulated pro-inflammatory cytokine and chemokines were associated with patients with predementia stage LLD and aMCI.. Our study showed that the model based on the pro-inflammatory cytokine could distinguish LLD from aMCI. These novel findings will give new clues to provide new therapeutic target for intervention or a marker for two diseases with similar pre-dementia syndromes.
Impacts of Public Telecommunications Policy on Industrial Geography and Welfare
Authors: Chatti Walid , Abalala Turki 
Published: June 2021

Abstract
We include a clear distinction between transport and telecommunication infrastructures. We assume that public expenditure enables Information Technology Enabled Services to be traded abroad without the use of traditional transport modes. We show that the increase in the knowledge spillovers mainly related to mobile human capital and trade of services can develop industrialization in developing country, leading to less spatial inequalities. This latter must invest more in telecommunication than in transportation infrastructures to attract both industrial and knowledge activities. The welfare level will be improved for skilled workers in both countries when public policy decreases the cost of trading knowledge.
Engineered polyethersulfone membrane for well-dispersed silver nanoparticle impregnation at high loading: high water permeate flux and biofouling prevention
Authors: Mukherjee Mohana , Bandyopadhyaya Rajdip 
Published: June 2021

Abstract
We present a new method for impregnation of silver nanoparticles (Ag NPs) at high loading on polyethersulfone (PES) membrane's external surface, simultaneously retaining native membrane's porosity – to achieve a high water permeate flux without biofouling. This was possible by PES membrane's surface modification with acrylic acid (AA), finally leading to AA-Ag-PES membrane. AA-Ag-PES had a high (9.04%) Ag-NP loading selectively on membrane surface, as discrete, smaller (mean size: 20 nm) nanoparticles (NPs). In nonfunctionalized Ag-PES, aggregated (mean size: 70 nm) NPs, with lower Ag loading (0.73 wt.%) was obtained, with NP being present both on membrane surface and inside pores. Consequently, AA-Ag-PES could maintain similar water permeability and porosity (10,153.05 Lm−2 h−1bar−1 and 69.98%, respectively), as in native PES (11,368.74 Lm−2 h−1bar−1 and 68.86%, respectively); whereas both parameters dropped significantly for Ag-PES (4,869.66 Lm−2 h−1bar−1 and 49.02%, respectively). AA-Ag-PES also showed least flux reduction (7.7%) due to its anti-biofouling property and high flux recovery after usage and cleaning, compared to native PES and Ag-PES membrane's much higher flux reduction (54.29% and 36.7%, respectively). Hence, discrete NP impregnation, avoiding pore blockage, is key for achieving high water flux and anti-biofouling properties (in AA-Ag-PES), compared to non-functionalized Ag-PES, due to aggregated Ag-NPs inside its pores.
Association Between the Digital Clock Drawing Test and Neuropsychological Test Performance: Large Community-Based Prospective Cohort (Framingham Heart Study)
Authors: Yuan Jing , Libon David J , Karjadi Cody , Ang Alvin F A , Devine Sherral , Auerbach Sanford H , Au Rhoda , Lin Honghuang 
Published: June 2021

Background
The Clock Drawing Test (CDT) has been widely used in clinic for cognitive assessment. Recently, a digital Clock Drawing Text (dCDT) that is able to capture the entire sequence of clock drawing behaviors was introduced. While a variety of domain-specific features can be derived from the dCDT, it has not yet been evaluated in a large community-based population whether the features derived from the dCDT correlate with cognitive function.

Objective
We aimed to investigate the association between dCDT features and cognitive performance across multiple domains.

Methods
Participants from the Framingham Heart Study, a large community-based cohort with longitudinal cognitive surveillance, who did not have dementia were included. Participants were administered both the dCDT and a standard protocol of neuropsychological tests that measured a wide range of cognitive functions. A total of 105 features were derived from the dCDT, and their associations with 18 neuropsychological tests were assessed with linear regression models adjusted for age and sex. Associations between a composite score from dCDT features were also assessed for associations with each neuropsychological test and cognitive status (clinically diagnosed mild cognitive impairment compared to normal cognition).

Results
The study included 2062 participants (age: mean 62, SD 13 years, 51.6% women), among whom 36 were diagnosed with mild cognitive impairment. Each neuropsychological test was associated with an average of 50 dCDT features. The composite scores derived from dCDT features were significantly associated with both neuropsychological tests and mild cognitive impairment.

Conclusions
The dCDT can potentially be used as a tool for cognitive assessment in large community-based populations.
Profundidad de siembra y tamaño de las semillas: desarrollo de las plántulas de frijol Mungo
Authors: Faria Adriene Vieira de , Barbosa Kássia de Paula , Costa Adriana Rodolfo , Silva Patricia Costa , Drumond Anailda Angélica Lana , França Janaína Borges de Azevedo , Silva Gilzângela Martins 
Published: June 2021

Contextualización del tema: el frijol mungo es ampliamente cultivado en Asia y en Brasil, y ha surgido como una opción para el cultivo en el segundo cultivo en el Centro Oeste de Brasil, especialmente cuando se pretende la rotación con pastos; sin embargo, la aparición desigual y lenta de las plántulas de frijol mungo puede conducir a retrasos en el desarrollo del cultivo y perjudicar la posición final.

Vacío de investigación: algunas de las condiciones que afectan al establecimiento inicial del cultivo son la profundidad de siembra y el tamaño de la semilla. Estos factores todavía están siendo discutidos en el entorno científico, especialmente para una cultura recientemente insertada en Brasil.

Propósito del estudio: el objetivo fue evaluar el efecto del tamaño de las semillas y la estandarización de la profundidad de la siembra en el rendimiento de las plántulas de frijol mungo.

Metodología: el ensayo se llevó a cabo en un Latossuelo Rojo, con clima Aw, precipitación media anual de 1300 mm y distribución bimodal. El diseño experimental utilizado fue completamente aleatorio, en un esquema factorial de 2 × 3 con 8 réplicas de 25 semillas, con un total de 48 parcelas. Los factores analizados fueron de los tamaños de semilla (3 y 4 mm) y tres profundidades de siembra (1, 2 y 3 cm). Se evaluó el índice de velocidad de emergencia (IVE), las plántulas no emergidas, las plántulas normales, la masa fresca, la masa seca y la longitud de las plántulas. Los datos se sometieron al análisis de varianza y, cuando se aplicaba una probabilidad significativa del 5%, se aplicó una prueba de Tukey para comparar los medios de los factores en estudio.

Resultados y conclusiones: se verificó que, cuando se siembran a 1 cm de profundidad, las semillas de frijol mungo tienen IVE más bajo; sin embargo, obtuvieron un menor porcentaje de plántulas no relevantes para el estudio, y un mayor porcentaje de plántulas normales emergió a una profundidad de 3 cm. Por lo tanto, la profundidad más recomendada para la siembra de frijoles mungo fue de 2 cm, independientemente del tamaño de la semilla utilizada. Lo tamaño de semilla de 4 mm permitió el desarrollo de plántulas con mayor acumulación de materia seca.
Efek anthiperkolesterol snack bars beras ketan hitam (Oryza sativa var. glutinosa) labu kuning (Cucurbita moschata) pada tikus Sprague dawley
Authors: Mustofa Akhmad , Suhartatik Nanik , Nugraheni Ratna Dewi 
Published: June 2021

Snack bars from black glutinous rice and yellow pumpkin powder has been developed. The aim of the research was to evaluate the effect of the snack bars to the lipid profile of white male rats Sprague dawley. The experimental design of the research was Pre-Post Test Randomized Control Group Design using 25 male white rats. The rats were divided randomly into 5 groups, which was Group 1 as negative control (standard feed), Group 2 as positive control (feed with lard for hyper cholesterolemia condition, Group 3 was treated with simvastatin (0.18 mg / 200gBB), Group 4 treated with snack bars A (450mg / kgBB) and Group 5 was treated with snack bars B (450mg / kg BB). The differences between snack bar A and B were the proportion of black glutinous rice and yellow pumpkin powder. The snack bar was given orally. Total cholesterol, HDL, LDL, and triglycerides after 14 days of the treatment. According to the statistical analysis (p<0.05), the results showed that group with simvastatin has no significant differences with group with snack bars A. Group with simvastatin, snack bars A, and snack bars B significantly influence blood cholesterol levels, total cholesterol, triglyceride levels, and LDL levels. Mainly, snack bars A could decline blood cholesterol as good as simvastatin. Snack bars A have a higher portion of black glutinous rice than snack bars B.
Heat Exchanger Handling MWCNT/Water Nanofluids
Authors: Alam Md Jasim , Baig Mr. Yasir 
Published: June 2021

Heat exchangers are an essential part in an assortment of mechanical settings, for example, cooling frameworks, force plants, refineries, and in this way ceaseless endeavor are made to expand their heat transfer efficiencies. Optimize design of helical coil heat exchanger by using fins and the Compare pressure & temperature by conventional design. The final outcome of the study increase the total heat transfer rate inside the domain. And increase the pressure drop inside the domain. The water outlet temperature decrease up to 315k and cold outlet temperature increase up to 320 k. and total pressure drop increase with the temperature increases. Finally the CFD data were compared with previous data the total pressure drop increase up to 0.65 bar for case-2.the overall efficiency of the system incites up to 5% to 6%.
Bewertung der Glaubwürdigkeit von CSR-Maßnahmen – Eine experimentelle Untersuchung zur Bedeutung von Engagement und CSR-Kommunikation am Beispiel eines fiktiven Unternehmens aus der Lebensmittelproduktion
Authors: Sattlberger Florian , Meixner Oliver 
Published: June 2021

Zusammenfassung
Der Faktor „Glaubwürdigkeit“ spielt in der unternehmerischen Kommunikation, speziell im Lebensmittelbereich, eine besonders wichtige Rolle. Es wurde untersucht, wie sich das Engagement (finanzielle und zeitliche Ausstattung eines Projekts) und die kommunizierten Interessen eines Unternehmens auf die Bewertung von CSR-Maßnahmen (Corporate Social Responsibility) hinsichtlich der Glaubwürdigkeit auswirken. Dies wurde anhand einer Stichprobe (n = 240) von 20- bis 35-jährigen Personen, wohnhaft in Österreich, untersucht. Die Personen wurden mithilfe eines Umfrageinstituts rekrutiert und zu je gleichen Teilen (Altersstruktur, Verteilung weiblich/männlich) randomisiert vier Gruppen zugeordnet. Damit soll ein größtmögliches Maß an interner Validität bzw. Vergleichbarkeit erreicht werden. Glaubwürdigkeit für die CSR-Maßnahmen definiert sich aus der Zuschreibung von Attributionen, grundsätzlichen Bewertungen von CSR-Maßnahmen, den Konstrukten Vertrauenswürdigkeit und Kompetenz sowie der Bewertung des Unternehmensimages. Die Autoren bedienen sich eines zweimal zwei-faktoriellen experimentellen Untersuchungsdesigns. Die Ergebnisse zeigen, dass das Engagement im Vergleich zu den kommunizierten Interessen bzw. Motiven, warum ein Unternehmen sich dem Thema „CSR“ widmet, entgegen den Erwartungen eine untergeordnete Rolle spielt. Daraus lässt sich ableiten, dass es im Zusammenhang mit CSR vor allem um eine ehrliche, transparente Kommunikation der Unternehmensziele geht, bei der die Unternehmen nicht nur auf ihre altruistischen Motive für die Gesellschaft verweisen, sondern auch darauf, welchen Nutzen das Unternehmen selbst aus CSR-Aktivitäten ziehen kann.
Educational intervention for the awareness improvement and control programme design on Echinococcosis in Izmir, Turkey
Authors: Altintas NA. , Altintas NU. , Sarica Yilmaz O. , Akil M. , Akdur Ozturk E. , Unver A. 
Published: June 2021

Summary
In Turkey, cyst hydatid disease (CHD) or cystic echinococcosis (CE) is publicly known as “dog cyst”, a fatal and serious disease not only affects livestock husbandry and human health but also brings about economic loss to our country. According to the data of the Ministry of Health; number of annual cases was 408 in 2008, and this number reached 1,867 by the end of 2019. Cystic echinococcosis is especially taken up during childhood and emerged at an older age. They become exposed to the eggs of the tapeworm after close contact with an infected dog or its contaminated environment. The infected dogs also pass in their feces E. granulosus eggs that adhere to the dogs’ hairs, and pass on to the children who are in the course of playful and intimate contact with the infected dogs. This study was to create the awareness of risk factors of CE among 10 different districts of Izmir province. Awareness raising seminars are essential component of this study because local people living in CE endemic areas, are crucial to continue and sustain the long-time effort that is needed to tackle this disease. In each district, 3 awareness raising seminars were held to the target groups: (a) in schools for students, teachers, administrators, (b) for general public, (c) for healthcare professionals. 4090 students attended to the trainings, 242 administrators and teachers who attended to the presentations together with the students, 524 people were attended to the trainings and 327 health workers attended to the trainings from different institutions. This study helped improving this situation by organising educational events for the rural populations for preventing transmission of the disease. This is the first educational intervention study regarding creating awareness on CE in Izmir Province which includes 10 districts between January 2019 to January 2020.
Mannerlaatta analogisen ja digitaalisen välissä
Authors: Kilpiö Juha-Pekka 
Published: June 2021

Artikkeli käsittelee Mika Taanilan ja Harry Salmenniemen kokeellista elokuvaa Mannerlaatta (2016), joka on tehty ilman kameraa. Mannerlaatassa vuorottelevat Taanilan kuvat ja Salmenniemen tekstit, ja se on valmistettu käyttäen sekä analogista filmiä että digitaalista teknologiaa. Pohdin erityisesti sen mediaalisia ominaispiirteitä tässä analogisen ja digitaalisen sekä kirjallisen ja elokuvallisen risteyskohdassa. Analyysi tarkentaa paitsi katsomiskokemukseen myös elokuvan valmistusprosessiin, ja siinä hyödynnetään media-arkeologiaa, jonka avulla analysoidaan, miten teknologiat eri ajoilta elävät teoksessa rinnakkain. Käsittelen ohessa myös korealais-amerikkalaisen Young-Hae Chang Heavy Industries -taiteilijaparin tekstuaalisia elokuvia, jotka on samoin toteutettu ilman kameraa. Vertailu tarkentaa Mannerlaatan asemaa digitaalisessa kulttuurissa.
Analisis Faktor Risiko Kejadian Tuberkulosis Paru Di Kecamatan Napabalano Kabupaten Muna
Authors: Hasriani Hasriani , La rangki La rangki , Fitriani Fitriani 
Published: June 2021

Data rekam medik di Puskesmas Napabalano kejadian TB paru tahun 2016 periode Januari - Desember sebanyak 315 suspek kasus, sedangkan pada tahun 2017 periode Januari - Desember suspek kejadian TB paru sebanyak 261 kasus.Tujuan penelitian ini untuk analisis faktor risiko kejadian penyakit TB Paru di Kecamatan Napabalano Kabupaten Muna. Desain penelitian adalah Case Control Study. Populasi penelitian adalahsemua suspek TB paru yang berada di Kecamatan Napabalano Kabupaten Muna mulai Januari sampai Desember Tahun 2017 berjumlah 261 kasus dengan jumlah sampel kasus 57 dan kontrol 57diambil dengan teknik Simple Random Sampling. Hasil penelitian menunjukkan bahwa kepadatan hunian merupakan faktor risiko kejadian TB Paru, responden yang berada dirumah dengan padat huniannya yaitu > 1 orang per 10 m2 berisiko menderita TB paru sebesar 6 kali dibandingkan dengan responden yang berada dirumah tidak padat huniannya yaitu < 1 orang per 10 m2.Status gizi merupakan faktor risiko kejadian TB Paru, responden dengan status gizi kurang nilai IMT <18 berisiko menderita TB paru sebesar 33 kali dibandingkan dengan responden dengan status gizi normal nilai IMT 18-24.Simpulan dari penelitian ini adalah ada faktor risiko kepadatan hunian, status gizi Saran perlu penyuluhan kepadatan hunian, status gizi dan perilaku pencegahan penyakit tuberkulosis. Kata Kunci: Risiko TB Paru, Kepadatan Hunian, Status Gizi