bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Plantation's Outsides: The Work of Settlement in Kalimpong, India
Authors: Besky Sarah 
Published: March 2021

AbstractWhile the colonial and contemporary economy of Bengal's Himalayan foothills is most often associated with the tea plantations of Darjeeling and the Dooars, the small farms of nearby Kalimpong were also a key space in which colonial agents and missionaries worked to “settle” the mountainous terrain. Focused on Kalimpong, this article traces the trajectory of one technology of settlement, agricultural extension, from the late 1880s to the early 1940s. It highlights agricultural extension's racialized and gendered politics, as well as its implication in a long-term project that merged material (i.e., food) provision with social reproduction (i.e., childrearing, kin-making). Agricultural extension created a patchwork of relatively biodiverse small farms that historical and contemporary accounts describe as a “green belt”: a socio-ecological outside to the plantation monocultures that dominate the hills. British governors attempted to use non-plantation space for multiple ends. In this sense, their work might be termed “biopolitical,” in that it was geared toward supporting and amplifying the life chances of certain human bodies and certain botanical species. Through a series of experiments, colonial agents made calculated choices about which of these forms of life should be made to flourish, and which might be allowed to perish. Importantly, settlement, as a set of intertwined projects, did not unfold in a coherent or deliberately sequential manner. Settlement was, and continues to be, a sedimentary process.
A new record of a cryptogenic Dipolydora species (Annelida: Spionidae) in South Africa
Authors: David Andrew A. , Williams Jason D. , Simon Carol A. 
Published: March 2021

AbstractIn this study we report a new record of a cryptogenic polychaete from southern Africa. The species was found inhabiting sand tubes in intertidal sand flats in the Knysna Estuary on the southern coast of South Africa. Morphological comparisons using light and scanning electron microscopy showed extensive taxonomic similarities with Dipolydora socialis described from other localities and from museum vouchers. In addition, 18S rRNA and COI barcodes were generated for the species. Genetic analysis of the assembled polydorid dataset corroborated the morphological data in delineating the species as a taxonomic unit with >99% genetic similarity to available sequences of D. socialis in the GenBank database. Dipolydora socialis has been reported as having a widespread distribution, and since it can reside within tubes associated with fouling communities or as a shell borer, several vectors may have been responsible for its global spread and introduction to southern Africa. Finally, considering the many cryptic complexes that are currently being uncovered within polychaetes, including spionids, future taxonomic studies should incorporate additional genetic data from other regions of the world to determine whether D. socialis may also be part of a larger species complex.
Vitamin D sufficiency attenuates the effect of early social adversity on child antisocial behavior
Authors: Choy Olivia , Raine Adrian 
Published: March 2021

AbstractBackgroundVitamin D insufficiency and child antisocial behavior are public health concerns. It is unknown whether vitamin D plays a role in antisocial outcomes. This study examines whether higher levels of vitamin D can act as a protective factor against antisocial behavior for children who are exposed to early social adversity.MethodsIn a community sample of 300 children aged 11–12 years (151 females, 149 males), serum concentrations of 25-hydroxyvitamin D [25(OH)D] were assessed alongside early social adversity, and both parent and child-reported antisocial behavior.ResultsVitamin D moderated the association between early social adversity and multiple antisocial outcomes. Higher social adversity was associated with greater antisocial behavior among vitamin D-insufficient [25(OH)D < 30 ng/mL], but not vitamin D-sufficient children [25(OH)D ⩾ 30 ng/mL], after adjusting for other variables. Results from child reports of antisocial behavior were replicated with parent reports, providing support for the robustness of the findings. At serum 25(OH)D concentrations above 27.16–30.69 ng/mL (close to 30 ng/mL, the recommended optimal vitamin D level for pediatric populations), the effect of social adversity on antisocial behavior outcomes was nullified.ConclusionsTo our knowledge, this study is the first to document that a nutritional factor, vitamin D, can potentially confer resilience to antisocial behavior. Our findings in a pediatric population suggest a possible role of vitamin D supplementation in interventions to reduce antisocial behavior, which may be further investigated in future randomized controlled trials.
Effect of sex and dyad composition on speech and gesture development of singleton and twin children
Authors: OZTURK Sumeyra , PINAR Ebru , KETREZ F. Nihan , ÖZÇALIŞKAN Şeyda 
Published: March 2021

AbstractChildren's early vocabulary shows sex differences – with boys having smaller vocabularies than age-comparable girls – a pattern that becomes evident in both singletons and twins. Twins also use fewer words than their singleton peers. However, we know relatively less about sex differences in early gesturing in singletons or twins, and also how singletons and twins might differ in their early gesture use. We examine the patterns of speech and gesture production of singleton and twin children, ages 0;10-to-3;4, during structured parent-child play. Boys and girls – singleton or twin – were similar in speech and gesture production, but singletons used a greater amount and diversity of speech and gestures than twins. There was no effect of twin dyad type (boy-boy, girl-girl, boy-girl) on either speech or gesture production. These results confirm earlier research showing close integration between gesture and speech in singletons in early language development, and further extend these patterns to twin children.
Heartbeats, Burdens, and Biofixtures
Authors: GIPE KELSEY 
Published: March 2021

AbstractThis paper addresses a dichotomy in the attitudes of some clinicians and bioethicists regarding whether there is a moral difference between deactivating a cardiac pacemaker in a highly dependent patient at the end of life, as opposed to standard cases of withdrawal of treatment. Although many clinicians hold that there is a difference, some bioethicists maintain that the two sorts of cases are morally equivalent. The author explores one potential morally significant point of difference between pacemakers and certain other life-sustaining treatments: specifically, that the former are biofixtures, which become part of the patient in a way that the latter do not. The concept of the pacemaker as biofixture grants pacemakers a unique moral status that gives reason to treat a pacemaker the same as other parts of the patient that are necessary to sustain life. The author employs this biofixture analysis to affirm the intuition that deactivating a pacemaker in a highly dependent patient at the end of life is, in moral terms, more analogous to active euthanasia than it is to standard cases of withdrawal of treatment. The paper concludes with consideration of potential implications for further implantable medical technologies, such as ventricular assist devices and total artificial hearts.
A Future for Migrants with Acute Heart Problems Seeking Asylum?
Authors: HERMERÉN GÖRAN 
Published: March 2021

AbstractThis paper discusses the future of migrants with acute heart problems and without permanent permission to remain in the country where they are seeking asylum. What does the country they have traveled to owe them? Specifically, what healthcare services are they entitled to? This may seem a niche problem, but numbers of migrants with acute heart problems could increase in the future. Besides, similar problems could be raised by, for instance, traumatized migrants with acute needs for healthcare services for other serious conditions. The paper identifies the issues and some positions on them. Arguments for and against these positions are explored. This particular set of problems in healthcare ethics creates several challenges, at both national and international levels, concerning access to transplantation, public willingness to donate organs, optimal use of organs, justice and fairness, and potential conflicts of law, politics and ethics, as well as issues revolving around interaction and communication (or lack of it) between agencies and professions.
School food offer at lunchtime: assessing the validity and reliability of a web-based questionnaire
Authors: Morin Pascale , Boulanger Amélie , Landry Myriam , Lebel Alexandre , Gagnon Pierre 
Published: March 2021

AbstractObjectives:To develop and validate a web-based self-diagnostic questionnaire on school food service offer aimed at food service managers (FSM) by: (i) identifying relevant indicators of school food offer, developing a questionnaire and validating the concept using an expert panel; (ii) validating the questions by comparing the FSM’s responses with observations by dietitians and (iii) undergoing a qualitative evaluation of the tool through direct observation and short interviews.Design:Mixed methods.Setting:Quebec, Canada.Participants:Nine experts validated the theoretical constructs and indicators on which the questionnaire was based. Inter-rater reliability tests were conducted with thirty-nine FSM, who then participated in interviews about platform functionality satisfaction. Twenty school stakeholders participated in the survey pertaining to their use of the personalised report.Results:The questionnaire focused on the main school food service’s lunchtime offer and comprised twenty-six questions. The overall strength of agreement was good, and all questions’ strengths of agreement were fair to excellent except for one question. Qualitative data reached saturation and showed that navigation through the questionnaire was fluid. Improvements were suggested to increase user-friendliness and simplicity of both the platform and questionnaire. Results from the survey showed that all respondents were either satisfied or very satisfied with their personalised report.Conclusions:We successfully developed and validated a web-based self-diagnostic questionnaire. The final version facilitates knowledge mobilisation with school stakeholders and offers a new opportunity for the assessment and surveillance of school food offer.
Strengthening health system building blocks: configuring post-COVID-19 scenario in Pakistan
Authors: Shaikh Babar Tasneem 
Published: March 2021

Abstract

Aim:
To gauge the level of preparedness of health system of Pakistan in the wake of Corona Virus Disease 2019 (COVID-19) pandemic.

Background:
The global COVID-19 outbreak and its subsequent repercussions and implications, after being declared as a pandemic by the World Health Organization (WHO), exposed all the inherent, lingering, and acute shortcomings of the health systems in many developing countries and Pakistan was no exception.

Methods:
A detailed literature review was done which included peer-reviewed articles on COVID-19 and health system, published in local and international journals, WHO and World Bank’s publications, and the documents and official reports of the government. Focus was to glean and cite strategies adopted by the developing countries in response to COVID-19 and to see the applicability of those which are feasible for Pakistan.

Findings:
Level of preparedness was minimal and the response to manage the outbreak was weak. Based on toll of the cases and number of deaths, this public health threat turned out to be a catastrophe beyond the controlling authority and capacity of the health system, and hence other sectors and agencies had to be engaged for devising a concerted and integrated response to deal with the emergency. Governance was disorderly, financing was inadequate, human resources were not trained, supplies and logistic were not stocked, information system was patchy, and research capacity was limited, and most of all the service delivery was in a biggest chaos of times. COVID-19 demanded to re-configure the health system of Pakistan.

Conclusion:
Improving the emergency preparedness of the hospitals is the foremost and an urgent need. A strong national public health system in Pakistan is needed to rapidly investigate and analyze the reports, assess the magnitude of the public health risk, share real-time information, and implement public health control measures in a concerted and systematic demeanor.
Active Faults' Geometry in the Gulf of Aqaba, Southern Dead Sea Fault, Illuminated by Multibeam Bathymetric Data
Authors: Ribot Matthieu , Klinger Yann , Jónsson Sigurjón , Avsar Ulas , Pons‐Branchu Edwige , Matrau Rémi , Mallon Francis L. 
Published: March 2021

AbstractDetailed knowledge of fault geometry is important for accurate seismic hazard assessment. The Gulf of Aqaba, which corresponds to the southern termination of the 1200‐km‐long Dead Sea fault system, remains one of the least known parts of this plate boundary fault, in large part due to its location offshore. Classically, the Gulf of Aqaba has been described as a succession of three pull‐apart basins. Here, building on a new multibeam bathymetric survey of the Gulf of Aqaba, we provide details about the geometry of the faults at the bottom of the gulf that controls its morphology. In particular, we identify a 50‐km‐long fault section that shows evidence of recent activation. We associate this fault section (Aragonese fault) with the main fault section that ruptured during the 1995 magnitude Mw7.3 Nuweiba earthquake. In the southern part of the gulf, bathymetry emphasizes the strike‐slip nature of the Arnona fault, while dip‐slip motion seems to be accommodated mostly by faults located along the eastern edge of the gulf. Considering the simple linear geometry of the Arnona fault and the absence of any large earthquake for several centuries, despite an average slip‐rate of ∼5 mm/yr, this fault should be considered as a significant candidate for an earthquake rupture of magnitude 7 or above in the near future.
Neddylation is required for presynaptic clustering of mGlu7 and maturation of presynaptic terminals
Authors: Kang Minji , Lee DoEun , Song Jae-man , Park Sunha , Park Da-ha , Lee Sanghyeon , Suh Young Ho 
Published: March 2021

AbstractNeddylation is a posttranslational modification in which NEDD8 is conjugated to a target substrate by cellular processes similar to those involved in ubiquitination. Recent studies have identified PSD-95 and cofilin as substrates for neddylation in the brain and have shown that neddylation modulates the maturation and stability of dendritic spines in developing neurons. However, the precise substrates and functional consequences of neddylation at presynaptic terminals remain elusive. Here, we provide evidence that the mGlu7 receptor is a target of neddylation in heterologous cells and rat primary cultured neurons. We found that mGlu7 neddylation is reduced by agonist treatment and is required for the clustering of mGlu7 in the presynaptic active zone. In addition, we observed that neddylation is not required for the endocytosis of mGlu7, but it facilitates the ubiquitination of mGlu7 and stabilizes mGlu7 protein expression. Finally, we demonstrate that neddylation is necessary for the maturation of excitatory presynaptic terminals, providing a key role for neddylation in synaptic function.
The deubiquitinase Usp9x regulates PRC2-mediated chromatin reprogramming during mouse development
Authors: Macrae Trisha A. , Ramalho-Santos Miguel 
Published: March 2021

AbstractPluripotent cells of the mammalian embryo undergo extensive chromatin rewiring to prepare for lineage commitment after implantation. Repressive H3K27me3, deposited by Polycomb Repressive Complex 2 (PRC2), is reallocated from large blankets in pre-implantation embryos to mark promoters of developmental genes. The regulation of this global redistribution of H3K27me3 is poorly understood. Here we report a post-translational mechanism that destabilizes PRC2 to constrict H3K27me3 during lineage commitment. Using an auxin-inducible degron system, we show that the deubiquitinase Usp9x is required for mouse embryonic stem (ES) cell self-renewal. Usp9x-high ES cells have high PRC2 levels and bear a chromatin and transcriptional signature of the pre-implantation embryo, whereas Usp9x-low ES cells resemble the post-implantation, gastrulating epiblast. We show that Usp9x interacts with, deubiquitinates and stabilizes PRC2. Deletion of Usp9x in post-implantation embryos results in the derepression of genes that normally gain H3K27me3 after gastrulation, followed by the appearance of morphological abnormalities at E9.5, pointing to a recurrent link between Usp9x and PRC2 during development. Usp9x is a marker of “stemness” and is mutated in various neurological disorders and cancers. Our results unveil a Usp9x-PRC2 regulatory axis that is critical at peri-implantation and may be redeployed in other stem cell fate transitions and disease states.
Apico-basal cell compression regulates Lamin A/C levels in epithelial tissues
Authors: Iyer K. Venkatesan , Taubenberger Anna , Zeidan Salma Ahmed , Dye Natalie A. , Eaton Suzanne , Jülicher Frank 
Published: March 2021

AbstractThe levels of nuclear protein Lamin A/C are crucial for nuclear mechanotransduction. Lamin A/C levels are known to scale with tissue stiffness and extracellular matrix levels in mesenchymal tissues. But in epithelial tissues, where cells lack a strong interaction with the extracellular matrix, it is unclear how Lamin A/C is regulated. Here, we show in epithelial tissues that Lamin A/C levels scale with apico-basal cell compression, independent of tissue stiffness. Using genetic perturbations in Drosophila epithelial tissues, we show that apico-basal cell compression regulates the levels of Lamin A/C by deforming the nucleus. Further, in mammalian epithelial cells, we show that nuclear deformation regulates Lamin A/C levels by modulating the levels of phosphorylation of Lamin A/C at Serine 22, a target for Lamin A/C degradation. Taken together, our results reveal a mechanism of Lamin A/C regulation which could provide key insights for understanding nuclear mechanotransduction in epithelial tissues.
The box C/D snoRNP assembly factor Bcd1 interacts with the histone chaperone Rtt106 and controls its transcription dependent activity
Authors: Bragantini Benoît , Charron Christophe , Bourguet Maxime , Paul Arnaud , Tiotiu Decebal , Rothé Benjamin , Marty Hélène , Terral Guillaume , Hessmann Steve , Decourty Laurence , Chagot Marie-Eve , Strub Jean-Marc , Massenet Séverine , Bertrand Edouard , Quinternet Marc , Saveanu Cosmin , Cianférani Sarah , Labialle Stéphane , Manival Xavier , Charpentier Bruno 
Published: March 2021

AbstractBiogenesis of eukaryotic box C/D small nucleolar ribonucleoproteins initiates co-transcriptionally and requires the action of the assembly machinery including the Hsp90/R2TP complex, the Rsa1p:Hit1p heterodimer and the Bcd1 protein. We present genetic interactions between the Rsa1p-encoding gene and genes involved in chromatin organization includingRTT106that codes for the H3-H4 histone chaperone Rtt106p controlling H3K56ac deposition. We show that Bcd1p binds Rtt106p and controls its transcription-dependent recruitment by reducing its association with RNA polymerase II, modulating H3K56ac levels at gene body. We reveal the 3D structures of the free and Rtt106p-bound forms of Bcd1p using nuclear magnetic resonance and X-ray crystallography. The interaction is also studied by a combination of biophysical and proteomic techniques. Bcd1p interacts with a region that is distinct from the interaction interface between the histone chaperone and histone H3. Our results are evidence for a protein interaction interface for Rtt106p that controls its transcription-associated activity.
Qualitative similarities and differences in visual object representations between brains and deep networks
Authors: Jacob Georgin , Pramod R. T. , Katti Harish , Arun S. P. 
Published: March 2021

AbstractDeep neural networks have revolutionized computer vision, and their object representations across layers match coarsely with visual cortical areas in the brain. However, whether these representations exhibit qualitative patterns seen in human perception or brain representations remains unresolved. Here, we recast well-known perceptual and neural phenomena in terms of distance comparisons, and ask whether they are present in feedforward deep neural networks trained for object recognition. Some phenomena were present in randomly initialized networks, such as the global advantage effect, sparseness, and relative size. Many others were present after object recognition training, such as the Thatcher effect, mirror confusion, Weber’s law, relative size, multiple object normalization and correlated sparseness. Yet other phenomena were absent in trained networks, such as 3D shape processing, surface invariance, occlusion, natural parts and the global advantage. These findings indicate sufficient conditions for the emergence of these phenomena in brains and deep networks, and offer clues to the properties that could be incorporated to improve deep networks.
Hinge-shift mechanism as a protein design principle for the evolution of β-lactamases from substrate promiscuity to specificity
Authors: Modi Tushar , Risso Valeria A. , Martinez-Rodriguez Sergio , Gavira Jose A. , Mebrat Mubark D. , Van Horn Wade D. , Sanchez-Ruiz Jose M. , Banu Ozkan S. 
Published: March 2021

AbstractTEM-1 β-lactamase degrades β-lactam antibiotics with a strong preference for penicillins. Sequence reconstruction studies indicate that it evolved from ancestral enzymes that degraded a variety of β-lactam antibiotics with moderate efficiency. This generalist to specialist conversion involved more than 100 mutational changes, but conserved fold and catalytic residues, suggesting a role for dynamics in enzyme evolution. Here, we develop a conformational dynamics computational approach to rationally mold a protein flexibility profile on the basis of a hinge-shift mechanism. By deliberately weighting and altering the conformational dynamics of a putative Precambrian β-lactamase, we engineer enzyme specificity that mimics the modern TEM-1 β-lactamase with only 21 amino acid replacements. Our conformational dynamics design thus re-enacts the evolutionary process and provides a rational allosteric approach for manipulating function while conserving the enzyme active site.