bluuebunny/crossref_metadata_2025_split huggingface dataset:
Técnica de implante e exequibilidade, “in vitro” em aortas porcinas, de anastamose vascular término-terminal
Authors: Tenório Euclides Martins , Moraes Neto Fernando Ribeiro de , Granja Filho Luiz Gonzaga , Torres João Henrique de Andrade , Correia Patricia Eduardo Teles , Pitta Guilherme Benjamim Brandão , Reis Rosane Pereira dos 
Published: November 2021

Objetivo deste estudo foi demonstrar a exequibilidade, “in vitro”, em aortas porcinas, de anastomose vascular término-terminal, com UnitaryAnastomotic Device (UAD®), de titânio e descrever a técnica de implante. Trata-se de um estudo experimental, “in vitro” realizado no Núcleo de Cirurgia Experimental da Universidade Federal de Pernambuco. Os aspectos externos e internos das anastomoses vasculares apresentaram: Ausência de cotovelo ou abaulamento nas anastomoses e manutenção do eixo longitudinal do vaso, bem como integridade macroscópica do endotélio vascular, bordas regulares, ausência de fio de sutura no local das anastomoses , ausência de irregularidades e rugosidade. Com base neste estudo experimental pode-se concluir que as Anastomoses Término-terminais “in vitro”, com aortas porcinas e prótese em titânio - UAD®- Unitary Anastomotic Device: Não apresentaram deformações externas à pressão de até 420 mmHg; Externamente mantiveram a regularidade, sem acotovelamentos, sem abaulamentos, sem deformidades no plano longitudinal da aorta, entre os cabos proximal e distal à anastomose; Internamente, todas as anastomoses mantiveram a forma circular, bordos regulares, endotélio íntegro macroscopicamente, ausência completa de fios de sutura , ausência de rugosidades e lacerações e todas as anastomoses resistiram a 420mmHg, sem apresentar vazamentos.
Estudo da influência do estágio de lactação na concentração dos principais ácidos graxos de lactante com bebê nascido a termo
Authors: Castro Matheus Campos , Alves Eloize Silva , Saqueti Bruno Henrique Figueiredo , Ferreira Cintia Stefhany Ripke , Alves Jéssica Souza , Costa Joice Camila Martins , Santos Oscar de Oliveira , Visentainer Jesui Vergilio 
Published: November 2021

O presente estudo tem como objetivo avaliar a influência dos estágios de lactação (colostro, transição e maduro) na composição dos principais ácidos graxos e somatórios de ácidos graxos no leite humano de lactante com bebê nascido a termo. Para isso foi coletado de forma individualizada, no mesmo período do dia, e após 1 hora da mama ser esvaziada, por meio de ordenha manual cerca de 5 ml de leite humano, em seguida foi realizada a composição de ácidos graxos por meio de cromatografia em fase gasosa acoplado a detector de ionização em chama (CG-DIC). O ácido graxo encontrado majoritariamente para o colostro foi o ácido linoleico (18:2n-6), já para os leites de transição e maduro foi o ácido oleico (18:1n-9), além disso, todos os ácidos graxos essenciais e estritamente essenciais foram identificados e quantificados nas três fases. Foi possível observar que o estágio de lactação influencia de forma direta na composição de ácido graxo, sendo que o leite colostro é o que apresenta maior distinção entre as fases de lactação, e o leite de transição e o leite maduro tem mais similaridade entre si.
Fatores associados a estresse, ansiedade e depressão em pacientes com doenças crônicas durante o período de distanciamento social
Authors: Souza Gustavo Fonseca de Albquerque , Praciano Gabriella de Almeida Figueredo , Revorêdo Laís Maciel Yamamoto , Ramos Isabelle Thays de Freitas , Ferreira Neto Otávio da Cunha , Rego Juliana Soares de Oliveira , Costa Gabriella Viana da , Medeiros Luya de Albuquerque , Souza Alex Sandro Rolland 
Published: November 2021

Objetivo: identificar fatores associados ao estresse, ansiedade e depressão grave/extrema em doentes crônicos. Métodos: estudo de corte transversal realizado em 2020, utilizando a plataforma Google Forms contendo características sociodemográficas, hábitos de vida, questões sobre a COVID-19 e a escala de estresse, ansiedade e depressão (DASS-21). Incluíram-se 1.274 brasileiros doentes crônicos. Aplicou-se o modelo de regressão logística multivariado com nível de significância 5%. Resultados: possuir ≤ 44 anos, antecedentes de ansiedade e depressão, ser estudante da graduação, em isolamento social, usar medicações para dormir e reduzir/não realizar estudo, trabalho remoto e atividades de lazer possuem risco de estresse, ansiedade e depressão grave/extrema. Enquanto que ter doença cardiovascular foi protetivo para estresse (OR 0,5; IC95 % 0,35-0,79; p=0,002) e depressão (OR 0,3; IC95% 0,22-0,53; p<0,0001) grave/extrema. Conclusão: apesar dos fatores de riscos relacionados aos doentes crônicos durante o distanciamento social, doenças cardiovasculares associou-se à proteção de transtornos mentais.
HUBUNGAN USIA DENGAN TINGKAT PENGETAHUAN PENGAKSES PROGRAM KESEHATAN REPRODUKSI LANSIA MELALUI RADIO DI RETJOBUNTUNG YOGYAKARTA TAHUN 2020
Authors: J Nugrahaningtyas W Utami Chici Riansih, 
Published: November 2021

Pengetahuan tentang kesehatan reproduksi merupakan hal yang sangat penting, baik untuk remaja maupun lansia. Kenyataan masih banyak remaja dan lansia yang belum mengetahui mengenai kesehatan reproduksinya dengan baik. Pengetahuan tersebut dapat diperoleh dari berbagai sumber yang salah satunya adalah media massa. Tujuan penelitian ini untuk mengetahui seberapa banyak orang mengakses pengetahuan kesehatan reproduksi pada lansia. Metode penelitian ini deskriptif analitik dengan pendekatan cross sectional. Teknik sampling yang digunakan total sampling, data yang digunakan adalah data sekunder dengan populasi seluruh pemiarsa yang mengakses program kesehatan reproduksi di Radio Retjobuntung di Yogyakarta pada bulan Desember 2020. Analisis data menggunakan uji korelasi Chi Square. Hasil dalam penelitian ini pengakses program kesehatan reproduksi lansia lebih besar usia produktif dibandingkan dengan lansia dan jumlah pengakses laki-laki lebih besar dibandingkan jumlah pengakses perempuan. Sebagian besar memiliki tingkat pengetahuan yang cukup yaitu 21 responden (51,2%). Nilai yang signifikan dengan (p-value 0.001) (p0.05) berarti ada hubungan yang bermakna antara usia dengan tingkat pengetahuan pengakses siaran kesehatan reproduksi di radio Retjobuntung Yogyakarta. Kesimpulan pengetahuan kesehatan reproduski masih dibutuhkan oleh orang berusia < 60 tahun meskipun di program kesehatan reproduksi lansia. Selain itu topik kesehatan secara umum yang di akses di media massa radio adalah sama baik jenis kelamin, dan usia baik lansia maupun remaja.
EDUKASI 3 M (MEMAKAI MASKER, MENCUCI TANGAN, MENJAGA JARAK) DAN BERITA HOAX KEPADA ANAK-ANAK RW 010 KELURAHAN CAWANG, JAKARTA TIMUR
Authors: SIANIPAR IMELDA MASNI JUNIATY 
Published: November 2021

Virus Corona atau dikenal sebagai COVID-19 sedang menghantui dunia. Bermula dari Cina, ia menyebar ke seluruh dunia. Meski telah menyebabkan banyak kematian, faktanya banyak orang yang masih kurang peduli dengan bahaya virus Corona termasuk di Indonesia. Hal tersebut tidak hanya nampak pada perilaku orang dewasa namun juga pada anak-anak. Program pengabdian kepada masyakarat yang dilakukan oleh para dosen dan mahasiswa dari Program studi Ilmu Hubungan Internasional dan Ilmu Komunikasi Fakultas Ilmu Sosial dan Ilu Politik, Universitas Kristen Indoensia berupaya untuk meningkatkan kesadaran anak-anak di RW 10 kelurahan cawang, Jakarta Timur akan Perilaku Hidup Bersih dan Sehat (PHBS). Edukasi 3 M bertujuan untuk meningkatkan kesadaran anak untuk selalu memakai masker di ruang publik, rajin mencuci tangan dengan sabun dan menjaga jarak sementara edukasi berita hoax bertujuan untuk mengingatkan anak-anak agar waspada terhadap berita yang mereka konsumsi dari media TV dan media online. Metode yang digunakan dalam kegiatan ini adalah dengan metode presentasi dan perlombaan. PKM dilaksanakan secara onsite dan online. PKM ini diikuti oleh 60 anak berusia 6 sampai 11 tahun. Hasil dari edukasi ini adalah tumbuhnya kesadaran anak-anak di RW 10 kelurahan cawang, Jakarta Timur untuk menggunakan masker, rajin mencuci tangan, menjaga jarak dan mengenali berita - berita Hoax. Selain itu, perlombaan seperti melukis di atas masker, menggambar, mewarnai dan membuat video tentang aku dan corona berhasil membangkitkan semangat anak-anak untuk beraktivitas setelah menjalani pendidikan jarak jauh sejak bulan Maret 2020.
Kata kunci: virus Covid-19, 3M, Hoax, anak – anak Rw 10 kelurahan cawang Jakarta Timur
SYNTHESIS AND SPECTRAL CHARACTERISTICS OF Cu(II), Ni(II) AND Fe(III) NANOSIZED COM­PLEXES ON THE SURFACE OF CARBON QUANTUM DOT
Authors: Ogenko Volodymyr , Orysyk Svitlana , Kharkova Ljudmila , Yanko Oleg , Chen Dongchu 
Published: November 2021

Processes of interaction between carbon quantum dots (CQDs) and solutions of Cu(II) Ni(II) and Fe(III) chlorides in the surface layer have been investigated by electron and IR spectroscopy.
When hydrochloric acid is added to the aqueous suspension of CQDs, there is a signi­ficant batochromic shift of the average absorption band (AB) by 1285 cm-1 with a decrease in its intensity to ε = 23.39. The presence of copper in the suspension of CQDs at room temperature leads to a decrease in the intensity of this AB (ε = 21.80), which indicates the interaction of CQDs with metal ions.
After heating the suspension for 1 and 3 hours, the gypsochromic shift of this ABs (by 335 cm-1) to 27790 cm-1 with a decrease in intensity depending on the heating time was recorded. Such changes in the UV–Vis Spectrum are due to the redistribution of the electron density of electron transitions n → π *due to the coordination of functional groups with metal ions and the appearance of transitions with charge transfer from ligand to metal (CQD→Cu2+).
When heating the suspensions significantly increases the absorption intensity of the AB at 22070 cm-1: from ε = 4.59 to ε = 6.75, which indicates the formation of transitions with charge transfer from ligand to metal (ChTLM) due to the coordination of copper ions with CQD.
In the absorption spectra of CQD suspensions with NiCl2 before heating, a hypsochromic shift of AB at 27305 cm-1 by 150 cm-1 and an increase in the intensity of its to ε = 4.95 were registered. That is, Ni(II) ions also form coordination bonds with functional groups on the periphery of the CQD.
After heating hydrochloric acid suspensions of CQD with FeCl3, in contrast to the chlorides of previous metals, in the UV-region registered shoulder-shaped AB at 31545 cm-1, the intensity of which increases with heating time (from ε = 9.59 to ε = 12.10), and in the visible region, a weakly intense shoulder-shaped AB at 19345 cm-1 (ε = 3.71 and 4.58), associated with the presence of dd-electron transitions in the metal ion.
Such changes in the absorption spectra are explained by the fact that iron may interact with CQD in different ways (in addition to coordination with functional donor groups, the formation of coordination bonds with the π-electron system of conjugated CQDs bonds), which leads to additional weak shoulder-like AB at 31545 cm-1.
The IR-spectra data of CQDs showed the presence of a number of characteristic ABs for functionalized CQDs: ν(N–H) at 3260 сm1, (C=O) at 1830, 1840 and 1850 сm1, –С=O(NH) at 1770 сm1, ν(C=N) at 1680 and δ(N–H) at 1640 сm1 and 320-360 см-1 СП ν(Cu–Cl, Ni–Cl, Fe–Cl), which confirms the coordination of metals on the surface of CQDs.
MFAP4 Deficiency Attenuates Angiotensin II-Induced Abdominal Aortic Aneurysm Formation Through Regulation of Macrophage Infiltration and Activity
Authors: Pilecki Bartosz , de Carvalho Paulo V. S. D. , Kirketerp-Møller Katrine L. , Schlosser Anders , Kejling Karin , Dubik Magdalena , Madsen Nicklas P. , Stubbe Jane , Hansen Pernille B. L. , Andersen Thomas L. , Moeller Jesper B. , Marcussen Niels , Azevedo Vasco , Hvidsten Svend , Baun Christina , Shi Guo-Ping , Lindholt Jes S. , Sorensen Grith L. 
Published: November 2021

Objective: Abdominal aortic aneurysm (AAA) is a common age-related vascular disease characterized by progressive weakening and dilatation of the aortic wall. Microfibrillar-associated protein 4 (MFAP4) is an extracellular matrix (ECM) protein involved in the induction of vascular remodeling. This study aimed to investigate if MFAP4 facilitates the development of AAA and characterize the underlying MFAP4-mediated mechanisms.Approach and Results: Double apolipoprotein E- and Mfap4-deficient (ApoE−/−Mfap4−/−) and control apolipoprotein E-deficient (ApoE−/−) mice were infused subcutaneously with angiotensin II (Ang II) for 28 days. Mfap4 expression was localized within the adventitial and medial layers and was upregulated after Ang II treatment. While Ang II-induced blood pressure increase was independent of Mfap4 genotype, ApoE−/−Mfap4−/− mice exhibited significantly lower AAA incidence and reduced maximal aortic diameter compared to ApoE−/− littermates. The ApoE−/−Mfap4−/− AAAs were further characterized by reduced macrophage infiltration, matrix metalloproteinase (MMP)-2 and MMP-9 activity, proliferative activity, collagen content, and elastic membrane disruption. MFAP4 deficiency also attenuated activation of integrin- and TGF-β-related signaling within the adventitial layer of AAA tissues. Finally, MFAP4 stimulation promoted human monocyte migration and significantly upregulated MMP-9 activity in macrophage-like THP-1 cells.Conclusion: This study demonstrates that MFAP4 induces macrophage-rich inflammation, MMP activity, and maladaptive remodeling of the ECM within the vessel wall, leading to an acceleration of AAA development and progression. Collectively, our findings suggest that MFAP4 is an essential aggravator of AAA pathology that acts through regulation of monocyte influx and MMP production.
Open-Label Placebo Treatment for Acute Postoperative Pain (OLP-POP Study): Study Protocol of a Randomized Controlled Trial
Authors: Sezer Dilan , de Leeuw Matthijs , Netzer Cordula , Dieterle Markus , Meyer Andrea , Buergler Sarah , Locher Cosima , Ruppen Wilhelm , Gaab Jens , Schneider Tobias 
Published: November 2021

Introduction: Open-label placebos have been proposed as way of using long recognized analgesic placebo effects in an ethical manner. Recent evidence shows efficacy of open-label placebos for clinical conditions, but there is need for more research on open-label placebos in acute pain. In the treatment of acute postoperative pain, minimization of opioid related side effects remains one of the key challenges. Therefore, this study aims at investigating the potential of adding unconditioned open-label placebos to treatment as usual as a means of reducing opioid consumption and its related side effects in patients with acute postoperative pain.Methods and Analysis: This is the protocol of an ongoing single site randomized controlled trial. The first patient was enrolled in May 2020. In total, 70 patients suffering from acute postoperative pain following dorsal lumbar interbody fusion are randomized to either a treatment as usual group or an experimental intervention group. The treatment as usual group consists of participants receiving a patient-controlled morphine pump. On day 1 and 2 post-surgery, patients in the intervention group receive, in addition to treatment as usual, two open-label placebo injections per day along with an evidence-based treatment rationale explaining the mechanisms of placebos. The primary outcome is measured by means of self-administered morphine during day 1 and 2 post-surgery. Several other outcome measures including pain intensity and adverse events as well as potential predictors of placebo response are assessed. Analysis of covariance will be used to answer the primary research question and additional statistical techniques such as generalized linear mixed models will be applied to model the temporal course of morphine consumption.Discussion: This study will provide valuable insights into the efficacy of open-label placebos in acute pain and will potentially constitute an important step toward the implementation of open-label placebos in the clinical management of acute postoperative pain. In addition, it will shed light on a cost-efficient and patient-centered strategy to reduce opioid consumption and its related side effects, without any loss in pain management efficacy.Ethics and Dissemination: The “Ethikkommission Nordwest- und Zentralschweiz” (BASEC2020-00099) approved the study protocol. Results of the analysis will be submitted for publication in a peer-reviewed journal.Clinical Trial Registration: The study is registered at ClinicalTrials.gov (NCT04339023) and is listed in the Swiss national registry at kofam.ch (SNCTP000003720).
Curiosity in Online Video Concept Learning and Short-Term Outcomes in Blended Medical Education
Authors: Ho Cheng-Maw , Yeh Chi-Chuan , Wang Jann-Yuan , Hu Rey-Heng , Lee Po-Huang 
Published: November 2021

Background: A student's level of curiosity in a subject after learning about it through online videos has not been addressed well in the medical education field. The purpose of this study, therefore, was to investigate online learning's effect on the stimulation of curiosity and short-term learning outcomes in a blended framework of precision medical education.Methods: A mixed-methods research design was used. During the 2020 academic year, all fifth-year medical students who, prior to class, viewed 6 video clips that presented 6 core concepts were invited to complete a survey and self-reflection on their learning process to assess their level of curiosity in each concept. For each group of medical students, teaching assistants helped collect anonymous survey data and summative assessment scores representing the students' learning outcomes. Video-viewing patterns, attained through an action log transformation, were also coded for analysis. Mann–Whitney U and Kruskal–Wallis tests were employed to compare differences between groups, and multiple linear regression was used to select the factors affecting learning outcomes. Qualitative data were content-coded through a descriptive approach using thematic analysis.Results: Of 142 medical students, 136 watched the online videos, 124 responded to the questionnaires, and 92 provided comments. Students' curiosity levels after learning about each concept through online videos significantly correlated with the degree to which a concept was learned. Medical students spent a median of 1.6 h online, and pause frequency correlated with curiosity in certain concepts. Aroused curiosity was associated with short-term learning outcomes in inconsistent effect sizes and directions. Students' feedback revealed various dimensions of curiosity, including novelty acknowledgment, recognition of an information gap, and information-seeking requests.Conclusions: Curiosity can be induced through online video learning platforms and has a role in short-term learning outcomes in medical education.
Comparison of the Efficacy and Safety of Minimally Invasive Simple Prostatectomy and Endoscopic Enucleation of Prostate for Large Benign Prostatic Hyperplasia
Authors: Li Jinze , Cao Dehong , Meng Chunyang , Xia Zhongyou , Peng Lei , Li Yunxiang , Wei Qiang 
Published: November 2021

Background: Minimally invasive simple prostatectomy (MISP) and endoscopic enucleation of the prostate (EEP) are the two most commonly used methods for large benign prostatic hyperplasia (BPH), but it remains unclear which of the two is superior. This study aims to perform a pooled analysis to compare efficacy and safety profiles between MISP and EEP.Methods: We conducted a comprehensive search of PubMed, Embase, Web of Science, and ClinicalTrials.gov databases to identify eligible studies comparing MISP with EEP. Parameters including efficacy and safety outcomes were compared using Stata 14.0 version.Results: Eight comparative trials with 1,504 patients were included. Compared to MISP, EEP demonstrated shorter operative time (mean difference [MD] 46.37, 95% confidence interval [CI] 19.92 to 72.82, p = 0.0006), lesser hemoglobin decrease (standardized MD [SMD] 0.59, 95% CI 0.23 to 0.95, p = 0.001), lower catheterization time (SMD 4.13, 95% CI 2.16 to 6.10, p &lt; 0.001), and shorter length of stay (SMD 2.38, 95% CI 1.40 to 3.36, p &lt; 0.001). However, overall complications and blood transfusions did not differ between the two groups. Moreover, EEP had better postvoid residual volume (PVR) at 6-month (MD 14.39, 95% CI 11.06 to 17.72, p &lt; 0.001) and comparable 3- and 6-month International Prostate Symptom Score, 3- and 6-month maximum flow rate, 3-month PVR, and 3-month quality of life compared with MISP.Conclusion: Both MISP and EEP are effective and safe surgical procedures for the treatment of large BPH. EEP appears to have a superior perioperative profile compared to MISP. This should be interpreted with caution due to the significant heterogeneity between studies. Hence, treatment selection should be based on the surgeon's experience and availability.
Hepatitis B Virus Promotes Hepatocellular Carcinoma Progression Synergistically With Hepatic Stellate Cells via Facilitating the Expression and Secretion of ENPP2
Authors: Deng Wanyu , Chen Fu , Zhou Ziyu , Huang Yipei , Lin Junlong , Zhang Fapeng , Xiao Gang , Liu Chaoqun , Liu Chao , Xu Leibo 
Published: November 2021

Background: Hepatitis B virus (HBV) infection is a major risk factor causing hepatocellular carcinoma (HCC) development, but the molecular mechanisms are not fully elucidated. It has been reported that virus infection induces ectonucleotide pyrophosphatase-phosphodiesterase 2 (ENPP2) expression, the latter participates in tumor progression. Therefore, the aim of the present study was to investigate whether HBV induced HCC malignancy via ENPP2.Methods: HCC patient clinical data were collected and prognosis was analyzed. Transient transfection and stable ectopic expression of the HBV genome were established in hepatoma cell lines. Immunohistochemical staining, RT-qPCR, western blot, and ELISA assays were used to detect the expression and secretion of ENPP2. Finally, CCK-8, colony formation, and migration assays as well as a subcutaneous xenograft mouse model were used to investigate the influence of HBV infection, ENPP2 expression, and activated hepatic stellate cells (aHSCs) on HCC progression in vitro and in vivo.Results: The data from cancer databases indicated that the level of ENPP2 was significant higher in HCC compared within normal liver tissues. Clinical relevance analysis using 158 HCC patients displayed that ENPP2 expression was positively correlated with poor overall survival and disease-free survival. Statistical analysis revealed that compared to HBV-negative HCC tissues, HBV-positive tissues expressed a higher level of ENPP2. In vitro, HBV upregulated ENPP2 expression and secretion in hepatoma cells and promoted hepatoma cell proliferation, colony formation, and migration via enhancement of ENPP2; downregulation of ENPP2 expression or inhibition of its function suppressed HCC progression. In addition, aHSCs strengthened hepatoma cell proliferation, migration in vitro, and promoted tumorigenesis synergistically with HBV in vivo; a loss-function assay further verified that ENPP2 is essential for HBV/aHSC-induced HCC progression.Conclusion: HBV enhanced the expression and secretion of ENPP2 in hepatoma cells, combined with aHSCs to promote HCC progression via ENPP2.