bluuebunny/crossref_metadata_2025_split huggingface dataset:
An Analysis of the Perspective of Last Year Medical Students in Training Allocated to Manage COVID-19 Hospital Patients in Mexico
Authors: Moreno Gabriela Y.C. , Ruiz Miguel Jesús Torres , Padilla Eleazar Lara , Zarazúa Rosalba Garduño , Vadillo Antonio Franco , Romero José de Jesús Peralta , Ramírez José Bruno Mendoza , Heald Adrian , Ramírez Javier Mancilla 
Published: June 2021

Abstract
BackgroundIn order to address the COVID-19 pandemic, health systems have used all their resources, including health care workers in training. Knowing the insights of these workers is of the utmost importance to generate adequate educative/political /administrative strategies. Methods An anonymous cross-sectional online survey was made by the General Directorate of Quality and Health Education in Mexico, in a convenience sample of 6,020 participants who belong to personnel in training for the health area, which included practitioners and professional technologists, undergraduate doctors, nursing, and residents in several specialties.ResultsDifferent positive and negative feelings were identified by the health workers who participated in facing this health emergency; emphasizing elements such as the need for Personal Protection Equipment (PPE) that, when deficient or lacking, generate concerns that raise questions about the medical/epidemiological attention to the pandemic. Based on an analysis of feelings, 8 main feelings were identified, which by frequency of appearing were: distrust(24.83%), fear(21.97%), sadness(12.45%), anticipation(11.65%), anger(10.71%), disgust(9.69%), joy(4.97%) and surprise(3.72%) which influence health workers in training and their wrok performance day to day.Concerning their positive and negative evaluation of their experience facing this health emergency, 13.83% of participants had a positive perception about participating in this kind of health emergency to support the country, 49.94% showed a negative evaluation, and 36.23% kept a neutral evaluation about their participation.ConclusionsThe health workers in training in Mexico gave a negative evaluation of the management of the health emergency. Distrust as a response to the absence of timely information from the education/health institutions, as well as concern about lack of personal protection equipment/inputs, are the main conflicts reported. We must establish a credible globally relevant continuity plan for the education of health care personnel in training, facing emergencies and disasters, so that next time we are properly prepared.
LMA ® Protector™ In Patients Undergoing Laparoscopic Surgeries: A Multicenter Prospective Observational Study
Authors: Liu Yanhong , Song Yuxiang , Wang Miaomiao , Yang Meihua , Shen Hao , Wang Zhen , Chen Liyong , Yang Jianjun , Gong Shengkai , Yu Yonghao , Shi Zhao , Zhang Wei , Zou Xuli , Sun Xude , Wang Yuan , Fu Qiang , Cao Jiangbei , Mi Weidong 
Published: June 2021

Abstract
Backgroundː Laryngeal masks airway (LMA) has been increasingly used in surgical patients. However, the use of LMA in laparoscopic surgeries remains controversial. The major concerns include the potential risk of esophageal regurgitation, aspiration, and difficulties to achieve effective ventilation. The aim of this study was to evaluate the safety and effectiveness of the LMA® Protector™ in patients undergoing laparoscopic surgery. Methodsː Patients aged 18 to 70 years, scheduled for laparoscopic surgeries were included. The insertion time, successful insertion rate, and oropharyngeal leak pressure were measured. Airway complications and airway manipulations during the procedure were documented. Effective ventilation rate was calculated. Visible bloodstains and reflux content in the drainage channel were documented after the removal of LMA® Protector™. Resultsː A total of 300 patients were enrolled. The insertion of LMA® Protector™ failed in seven patients resulting with a successful insertion rate of 97.7%. During the maintenance of anesthesia, airway manipulation was required in 19 patients (19/293, 6.48%), in three of whom the LMA was replaced with endotracheal intubation resulting with an effective ventilation rate of 96.7% (290/300). The oropharyngeal leak pressure was 30.18±5.88 cmH2O. 75 patients (25.86%) reported mild sore throat on the first day after surgery. No signs of aspiration were noticed in any of those patients. Conclusionsː The LMA® Protector™ was shown to be safe and effective in patients undergoing laparoscopic surgeries. Although minor complications that requiring no further treatment, no clinically diagnosed aspiration was noticed in our study. Trial registration: The trail was registered at the Chinese Clinical Trial Registry (ChiCTR1800018300, data of registration, September 2018).
White Matter Connectometry In Patients With Disorders of Consciousness Revealed By 7-Tesla Magnetic Resonance Imaging
Authors: Tan Xufei , Zhou Zhen , Gao Jian , Wei Ruili , Zhang Xiaotong , Luo Benyan 
Published: June 2021

Abstract
White matter disruption plays an important role in disorders of consciousness (DOC). The aim of this study was to analyze the connectometry between DOC patients and healthy controls and to explore the relationship between diffusion connectometry and levels of consciousness. Fourteen patients with DOC and 13 sex- and age-matched controls were included in this study. The participants underwent diffusion magnetic resonance imaging (MRI) and T1-weighted structural MRI at 7 Tesla. Diffusion MRI connectometry was performed to investigate the differences between groups, and to subsequently study the correlation between Coma Recovery Scale-Revised (CRS-R) scores and white matter integrity. In DOC patients, the quantitative anisotropy (QA) was significantly reduced in deep white matter tracts, whereas significantly higher QA values were found in the bilateral cerebellum compared with healthy controls. Moreover, the QA values in many tracts within the right hemisphere were higher in patients in a minimally conscious state compared to those in vegetative state/unresponsive wakefulness syndrome. In contrast, many tracts within the left hemisphere of the latter group showed higher QA than the former, which was reflected by the correlation between diffusion connectometry and CRS-R scores. These results indicate that the cerebellum may play an important role in DOC, and the lateralization of the cerebral hemisphere in affected patients may suggest neural compensation.
Extracellular Hsp90α is a Potential Serum Predictor of Atherosclerosis in type 2 Diabetes
Authors: Ding Xinyi , Meng Chuzhen , Dong Hangming , Zhang Shili , Zhou Hui , Tan Wenchong , Huang Lei , He Aiping , Li Jieyou , Huang Jiali , Li Wei , Zou Fei , Zou Mengchen 
Published: June 2021

Abstract
Background: Atherosclerosis is the main pathological change in diabetic angiopathy, and vascular inflammation plays an important role in early atherosclerosis. Heat shock protein 90, a cellular molecular chaperone, was recently determined to be secreted extracellularly, but the specific mechanism remains unclear. This study explored the relationship between Hsp90 and diabetic peripheral artery disease through serological analyses of different groups of diabetic patients and investigated the relationship between extracellular Hsp90α and vascular inflammation at the cellular level.Methods: Seventy-seven selected patients were divided into three groups. The relationships among serum Hsp90, oxidative stress indexes and patient outcomes and the correlations among the indexes were analysed. An oxidative stress endothelial injury model was established under high glucose in vitro to explore the role of eHsp90 release in atherosclerosis progression.Results: Serum Hsp90 and MDA levels tended to increase in different groups with peripheral vascular disease aggravation. Hsp90α was correlated with MDA to some extent and was predictive. In vitro, high glucose and low H2O2 treatment increased extracellular Hsp90 secretion, and endothelial cell conditioned medium and recombinant human Hsp90α increased monocyte migration (P<0.05).Conclusions: Extracellular Hsp90α participates in endothelial cell injury in diabetic vascular disease and initiates the inflammatory response by promoting monocyte migration.Trial registration: NCT04787770, ClinicalTrials.gov, Registered 9 March, 2021 - Prospective registered
Quality of Life &amp; Phonatory and Morphological Outcomes in Cognitively Unimpaired Adolescents With Pierre Robin Sequence – A Cross-sectional Study in a 72-patient Series
Authors: THOUVENIN Béatrice , SOUPRE Véronique , CAILLAUD Marie-Anne , Henry-Mestelan Charlotte , CHALOUHI Christel , HOUSSAMO Bachar , Chapuis Cécile , LIND Katia , ROYER Aurélie , VEGAS Nancy , AMIEL Jeanne , COULY Gérard , PICARD Arnaud , VAIVRE-DOURET Laurence , Abadie Véronique 
Published: June 2021

Abstract
We assessed the phonatory and morphological outcome of 72 cognitively unimpaired adolescents with Pierre Robin Sequence (PRS), studied their generic (Kidscreen-52), oral (COHIP-SF19) and vocal (VHI-9i) qualities of life (QoL), and sought to identify determinants of these outcomes. Two-thirds of our adolescents retained low or moderate phonation difficulties but risk factors for them were not identified. For 14%, esthetic results were considered disharmonious with no link to neonatal retrognathia severity. Only two-stage surgery appeared to have a role in improving esthetic results. The generic QoL of these adolescents was slightly lower than that of control patients, especially in dimensions concerning physical well-being, relationships and autonomy. Patients with non-isolated PRS had lower results than those with isolated PRS. Phonatory and morphological sequelae had no impact on generic QoL. Only non-isolated PRS and a low oral QoL had an impact on generic QoL. The oral QoL of these adolescents was comparable to that of control patients and significantly better than that of children with craniofacial malformations. The oral QoL of the adolescents with non-isolated PRS, was significantly worse than that of control patients and close to that of children with craniofacial malformations. The vocal QoL of our subjects was better than that of patients with other voice pathologies and better when phonation was good.
Association of Peripheral Blood Biomarkers with Response to anti-PD-1 Immunotherapy for Patients with dMMR Metastatic Colorectal Cancer: A Multicenter Cohort Study
Authors: Ye Shu-Biao , Chen Ping , Li Pei-Si , Lin Zhen-Sen , Chen Dong-Wen , Chen Shao-Xia , Cheng Yi-Kan , Lan M.D. Ping 
Published: June 2021

Abstract
Background: Mismatch repair deficient (dMMR) is an established biomarker for response to anti-PD-1 immunotherapy in metastatic colorectal carcinoma (mCRC). Although patients with dMMR mCRC could achieve a high incidence of disease control and favorable progression-free survival (PFS), reported response rates to PD-1 inhibitors are variable with 28%–52%, indicating that additional predictive biomarkers are warranted.Methods: This multicenter cohort study enrolled patients with dMMR mCRC receiving anti-PD-1 immunotherapy at Sun Yat-sen University, the Sixth Affiliated Hospital and Sun Yat-sen University Cancer Center between December, 2016 to December, 2019. A total of 20 peripheral blood biomarkers, including T cells (frequency of CD4+ T cell, frequency of CD8+ T cell, ratio of CD4+/CD8+), carcinoembryonic antigen (CEA), inflammatory markers and lipid metabolism markers. The association between response or survival and peripheral blood parameters was analyzed. Results: Among tested parameters, ratio of CD4+/CD8+, frequency of CD4+ T cell was significantly associated with PFS (P=0.023, P=0.012) and OS (P=0.027, P=0.019) in univariate analysis. Lower level of CD4+/CD8+ ratio or frequency of CD4+ T cell showed significant association with better overall response rates (ORR; P = 0.03, P=0.01). Ratio of CD4+/CD8+, and frequency of CD4+ T cell maintained significance in multivariate Cox model for PFS (HR=9.23, P=0.004; HR=4.83, P=0.02) and OS (HR=15.22, P=0.009; HR=16.21, P=0.025). Conclusions: Ratio of CD4+/CD8+ and frequency of CD4+ T cell might be crucial independent biomarkers within dMMR mCRC to better identify patients for response to PD-1 inhibitors. If validated in prospective clinical trials, ratio of CD4+/CD8+ and frequency of CD4+ T cell might provide aid in guiding the treatment of PD-1 inhibitors in dMMR mCRC.
SOX2 Knockdown with Sirna Reverses Cisplatin Resistance in NSCLC by Regulating APE1 Signaling
Authors: Chen Taiyu , Zhou Ji , Li Peng-cheng , Tang Chun-han , Yang Fang , Xu Ke , Li Tao , Ren Tao 
Published: June 2021

Abstract
Objective: SOX2 is related to drug resistance in many types of cancer, including lung cancer, but the underlying mechanism is still not fully understood. Herein, we investigated the role of SOX2 and its regulatory signaling in cisplatin-treated non-small-cell lung cancer (NSCLC).Methods: Cisplatin-resistant NSCLC A549/CDDP cells, parental A549 cells and NCI-H460 cells were selected for experiments. The effects of SOX2 on cell viability, proliferation, and apoptosis were evaluated in vitro. Western blotting, real-time quantitative PCR (qPCR), immunohistochemistry (IHC), and luciferase reporter assays were used to investigate the underlying mechanism of SOX2. Kaplan-Meier survival analysis and the log-rank test were used to assess the relationship between SOX2 expression levels and patient survival.Results: A549/CDDP cells had marked resistance to cisplatin and stronger colony formation ability than A549 cells. The expression of SOX2 protein or mRNA in A549/CDDP cells was higher than that in A549 cells. Knockdown of SOX2 in A549/CDDP cells induced apoptosis by inhibiting colony formation and decreasing viability, but overexpression of SOX2 reversed these effects. Interestingly, Genomatix software predicted that the APE1 promoter has some SOX2 binding sites, while the SOX2 promoter has no APE1 binding sites. Furthermore, luciferase reporter assays proved that SOX2 could bind the promoter of APE1 in 293T cells. We further verified that SOX2 expression was not affected by small hairpin RNA against APE1 (shAPE1) in A549/CDDP cells. As expected, colony formation was obviously inhibited and apoptosis was strongly enhanced in A549/CDDP cells treated with SOX2 small-interfering RNA (siSOX2) alone or combined with CDDP compared with control cells. Meaningfully, SOX2 expression was variable in 45 advanced NSCLC patients, and patients with high expression of SOX2 survived longer than those with low expression of SOX2.Conclusion: siSOX2 overcomes cisplatin resistance in NSCLC by regulating APE1 signaling, providing a new therapeutic target for overcoming cisplatin resistance in NSCLC patients.
Sexual Inactivity and Sexual Dissatisfaction Among Breast Cancer Survivors and Their Partners
Authors: Zhang Qian , Mu Qianqian , Fu Yan , Zhang Xiaoxia , Zheng  , Li Junying 
Published: June 2021

Abstract
PurposeTo measure the sexual activity of breast cancer survivors and their partners to better understand the causes of sexual inactivity and sexual dissatisfaction in this population.MethodsWe investigated the proportion of sexual activity and sexual dissatisfaction in a cross‐sectional study and described their association with sexual health information sexual thoughts, socio‐demographic factors, body image, marital satisfaction, and mental health problems. We enrolled 438 eligible couples by convenient sampling. The statistical analysis and graphical work were completed using SPSS and Graphpad Prism.ResultsOf all participants, 58.66% (257/438) reported being sexually inactive. Of the 41.3% (181/438) sexually active participants, 96.7% (175/181) were considered to have sexual dysfunction. Patients’ sexual knowledge related to their disease, such as “sexual activity may impede disease recovery (AOR = 1.642; 95% CI: 1.119~2.409)”, “sexual activity may cause cancer recurrence or metastasis (AOR = 1.526; 95% CI: 1.012~2.302)”, “sexual activity could change the estrogen level and stimulate tumor growth (AOR = 1.585; 95% CI: 1.021~2.460)” were significantly associated with sexual inactivity. Psychological issues related to sexual activity, and hospital's sexual health resources, such as“anxiety (AOR = 2.141; 95% CI: 1.400~3.272)”, “depression (AOR =2.082; 95% CI: 1.317~3.293)”, “feeling less feminine as a result of your disease or treatment (AOR = 1.526; 95% CI: 1.012~2.302) ”, “dissatisfied with their physical appearance (AOR = 1.514; 95% CI: 1.010~2.271) ”, “medical providers provide information on sexual health (AOR = 4.459; 95% CI: 2.044~9.730) ”, “used sexual health aids (AOR = 1.514; 95% CI: 1.010~2.271) ” were significantly associated with sexual dissatisfaction. We also identified that the sexual demands of the partner led to increased sexual dissatisfaction among the survivors. ConclusionsMost Chinese breast cancer survivors were sexually inactive. Patients’ sexual knowledge related to their disease was the great barrier to sexual activity. Improving psychological problems associated with sexual activity and providing professional sexual health resources in hospitals can effectively improve sexual satisfaction among survivors. In addition, the impact of the partner's proactive needs on sexual satisfaction also needs to be considered when developing couples' therapy together.
High-Density Genetic Linkage Map Construction Using Whole-Genome Resequencing for Mapping Qtls of Resistance to Aspergillus Flavus Infection in Peanut
Authors: Jiang Yifei , Luo Huaiyong , Yu Bolun , Ding Yingbin , Kang Yanping , Huang Li , Zhou Xiaojing , Liu Nian , Chen Weigang , Guo Jianbin , Huai Dongxin , Lei Yong , Jiang Huifang , Yan Liying , Liao Boshou 
Published: June 2021

Abstract
Cultivated peanut (Arachis hypogaea L.) is rich in edible oil and protein, which is widely planted around the world as an oil and cash crop. However, aflatoxin contamination seriously affects the quality safety of peanut, hindering the development of peanut industry and threatening consumers’ health. Breeding peanut varieties with resistance to Aspergillus flavus infection is important for control the aflatoxin contamination, and understanding of the genetic basis of resistance is vital to its genetic enhancement. In this study, we report the QTL mapping of resistance to A. flavus infection of a well-known resistant variety J11. A recombination inbred line (RIL) population was constructed by crossing a susceptible variety Zhonghua 16 and J11. Through whole-genome resequencing, a genetic linkage map was constructed with 2,802 recombination bins and an average inter-bin distance of 0.58 cM. Combined with phenotypic data of infection index in four consecutive years, six novel resistant QTLs were identified and they explained 5.03-10.87% phenotypic variances. The favorable alleles of five QTLs were from J11 while that of one QTL were from Zhonghua 16. The pyramiding of these favorable alleles significantly improved the resistance to A. flavus infection. These results could contribute greatly to understanding of genetic basis of A. flavus resistance and could be meaningful in further resistance improvement in peanut.
Delayed Bleeding at Different Anastomotic Sites After Operation for Synchronous Colon and Rectal Carcinoma: A Rare Case Report
Authors: Ling Chun-Run , Zhang Wen-Hua , Liu Zhi-Yong , Dong Chen Cheng 
Published: June 2021

Abstract
Background: Delayed postoperative anastomotic bleeding is a life-threatening complication of gastrointestinal reconstruction surgery, but rarely been reported in the literature. Case presentation: A 64-year-old man was admitted to our hospital with bleeding stool for 10 days, he had a long history of uncontrolled type 2 diabetes mellitus. He was then diagnosed with synchronous adenocarcinoma of the ascending colon and the rectum. Laparoscopic right hemicolectomy combined with low anterior resection of rectal cancer was conducted later. Delayed bleeding occured at ileocolonic anastomotic orifice and colorectal anastomotic orifice at different periods, by multi-means of hemostasis therapy, he fianlly gained a good recovery.Conclusion: Enhanced postoperative monitoring, early detection of bleeding and combined with multiple hemostasis methods are the keys to successful management of delayed postoperative anastomotic bleeding in colorectal cancer. Type 2 diabetes mellitus may be one of the risk factors of delayed postoperative anastomotic bleeding, and preventive ostomy may be beneficial to avoid the occurrence of delayed postoperative anastomotic bleeding, both of which needs to be confirmed by further studies.
Stability and Extraction of New Optical Wave Solutions of Cascaded System in (2 + 1)-dimensions
Authors: Kumar Hitender , Parveen Parveen , Dahiya Sunita , Kumar Anand , Gautam Manjeet Singh 
Published: June 2021

Abstract
This paper uses the new modified sub-ODE method, the new extended auxiliary equation method, and the new Jacobi elliptic function expansion method to revisit the (2+1)-dimensional coupled nonlinear Schr¨odinger equation with cascading effect. As a consequence, dark, bright, kinkantikink, singular solitons, Weierstrass elliptic function, doubly periodic, and complex optical soliton solutions are retrieved. All solutions are described, along with the existence criterion on the parameters. As solitons are used for data transfer, the obtained results may be found usage in optical couplers, birefringed fibres, and optoelectronic devices. A comparison of the obtained

results with those found in the literature is given. The dynamical behaviour of some of the obtained solutions has been explored for suitable choices of the parameters. Using the property of Hamiltonian systems, the solitons stability is determined.
Contactless generation and trapping of hydrodynamic knots in sessile droplets by acoustic screw dislocations
Authors: Song Shuren , Zhou Jia , Riaud Antoine , Marciano Antonino 
Published: June 2021

Abstract
Hydrodynamics knots are not only promising elementary structures to study mass and momentum transfer in turbulent flows, but also potent analogs for other topological problems arising in particle physics. However, experimental studies of knots are highly challenging due to the limited control over knot generation and difficult observation of the resulting fast-paced multiscale flow evolution. In this paper, we propose using acoustic streaming to tie hydrodynamic knots in fluids. The method is contactless, almost instantaneous and is relatively insensitive to viscosity. Importantly, it allows starting from quite arbitrary three dimensional flow structures without relying on external boundary conditions. We demonstrate our approach by using an acoustic screw dislocation to tie a knot in a sessile droplet. We observe an inversion of the knot chirality (measured by the hydrodynamic helicity) as the topological charge of the screw dislocation is increased. Combined with recent progress in acoustic field synthesis, this work opens a window to study more complex hydrodynamic knot topologies at a broader range of space and timescales.