bluuebunny/crossref_metadata_2025_split huggingface dataset:
Reduction in new cardiac electronic device implantations in Catalonia during COVID-19
Authors: Arbelo Elena , Angera Ignasi , Trucco Emilce , Rivas-Gándara Nuria , Guerra José M , Bisbal Felipe , Jáuregui-Abularach Miguel , Vallés Ermengol , Martin Gabriel , Sbraga Fabrizio , Tolosana José María , Linhart Markus , Francisco-Pascual Jaume , Montiel-Serrano José , Pereferrer Damià , Menéndez-Ramírez Diego , Jiménez Jesús , Elamrani Amin , Borrás Roger , Dallaglio Paolo Domenico , Benito Eva , Santos-Ortega Alba , Rodríguez-Font Enrique , Sarrias Axel , González-Matos Carlos E , Martí-Almor Julio , Cabrera Sandra , Mont Lluis 
Published: January 2021

AbstractAimsDuring the COVID-19 pandemic, concern regarding its effect on the management of non-communicable diseases has been raised. However, there are no data on the impact on cardiac implantable electronic devices (CIED) implantation rates. We aimed to determine the impact of SARS-CoV2 on the monthly incidence rates and type of pacemaker (PM) and implantable cardiac defibrillator (ICD) implantations in Catalonia before and after the declaration of the state of alarm in Spain on 14 March 2020.Methods and resultsData on new CIED implantations for 2017–20 were prospectively collected by nine hospitals in Catalonia. A mixed model with random intercepts corrected for time was used to estimate the change in monthly CIED implantations. Compared to the pre-COVID-19 period, an absolute decrease of 56.5% was observed (54.7% in PM and 63.7% in ICD) in CIED implantation rates. Total CIED implantations for 2017–19 and January and February 2020 was 250/month (&gt;195 PM and &gt;55 ICD), decreasing to 207 (161 PM and 46 ICD) in March and 131 (108 PM and 23 ICD) in April 2020. In April 2020, there was a significant fall of 185.25 CIED implantations compared to 2018 [95% confidence interval (CI) 129.6–240.9; P &lt; 0.001] and of 188 CIED compared to 2019 (95% CI 132.3–243.7; P &lt; 0.001). No significant differences in the type of PM or ICD were observed, nor in the indication for primary or secondary prevention.ConclusionsDuring the first wave of the COVID-19 pandemic, a substantial decrease in CIED implantations was observed in Catalonia. Our findings call for measures to avoid long-term social impact.
Therapy and outcomes of cardiac implantable electronic devices infections
Authors: Perrin Tilman , Deharo Jean-Claude 
Published: January 2021

Abstract
Cardiac implantable electronic device (CIED) infection causes significant morbidity and mortality without appropriate treatment. It can present as incisional infection, pocket infection, systemic CIED infection, or occult bacteraemia. Complete percutaneous CIED extraction (excepted in case of incisional infection) and appropriate antibiotic therapy are the two main pillars of therapy. Device reimplantation, if needed, should be delayed sufficiently to allow control of the infection. Here, we address the differences in prognosis according to the clinical scenario and the different treatment options.
Catheter ablation of atrial fibrillation in patients with hypertrophic cardiomyopathy: a European observational multicentre study
Authors: Creta Antonio , Elliott Perry , Earley Mark J , Dhinoja Mehul , Finlay Malcolm , Sporton Simon , Chow Anthony , Hunter Ross J , Papageorgiou Nikolaos , Lowe Martin , Mohiddin Saidi A , Boveda Serge , Adragao Pedro , Jebberi Zeynab , Matos Daniel , Schilling Richard J , Lambiase Pier D , Providência Rui 
Published: January 2021

Abstract

Aims
Atrial fibrillation (AF) is common in hypertrophic cardiomyopathy (HCM). Data on the efficacy of catheter ablation of AF in HCM patients are sparse.

Methods and results
Observational multicentre study in 137 HCM patients (mean age 55.0 ± 13.4, 29.1% female; 225 ablation procedures). We investigated (i) the efficacy of catheter ablation for AF beyond the initial 12 months; (ii) the available risk scores, stratification schemes and genotype as potential predictors of arrhythmia relapse, and (iii) the impact of cryoballoon vs. radiofrequency in procedural outcomes. Mean follow-up was 43.8 ± 37.0 months. Recurrences after the initial 12-month period post-ablation were frequent, and 24 months after the index procedure, nearly all patients with persistent AF had relapsed, and only 40% of those with paroxysmal AF remained free from arrhythmia recurrence. The APPLE score demonstrated a modest discriminative capacity for AF relapse post-ablation (c-statistic 0.63, 95% CI 0.52–0.75; P = 0.022), while the risk stratification schemes for sudden death did not. On multivariable analysis, left atrium diameter and LV apical aneurysm were independent predictors of recurrence. Fifty-eight patients were genotyped; arrhythmia-free survival was similar among subjects with different gene mutations. Rate of procedural complications was high (9.3%), although reducing over time. Outcome for cryoballoon and radiofrequency ablation was comparable.

Conclusion
Very late AF relapses post-ablation is common in HCM patients, especially in those with persistent AF. Left atrium size, LV apical aneurysm, and the APPLE score might contribute to identify subjects at higher risk of arrhythmia recurrence. First-time cryoballoon is comparable with radiofrequency ablation.
The influence of the root diseases Armillaria solidipes and Inonotus sulphurascens on the distribution of mule deer during winter
Authors: Carswell Brendan M , Rea Roy V , Rusch David , Johnson Chris J 
Published: January 2021

Abstract
Armillaria (Armillaria solidipes) and laminated root diseases (Inonotus sulphurascens) are two wide-ranging fungal pathogens that occur in the southern half of British Columbia (BC), Canada, and can infect economically and biologically important tree species such as interior Douglas-fir (Pseudotsuga menziesii var. glauca). In northern, temperate locations, Douglas-fir forests serve as winter habitat for ungulates. When these fungal infections are in Douglas-fir forests, core components of winter ranges are altered, including canopy cover, snowpack and understory vegetation. In this study, we investigated how Rocky Mountain mule deer (Odocoileus hemionus hemionus) of central BC used winter range habitats that included root disease (A. solidipes and I. sulphurascens) centres. We used remote camera-traps to collect data from September 2017 to April 2019, and we assessed those habitats in which the cameras were located during the summer and winter of 2018. We used logistic regression and an information theoretic approach to test a series of factors hypothesised to influence the use of root disease centres by mule deer. Our results show that mule deer use root disease centres less than control forests as well as negatively respond to the deeper snow packs found in root disease centres, especially in late winter. Our cameras also detected higher vertebrate diversity in root disease centres. We suggest that forest policy-makers should acknowledge heterogeneous habitat features such as root disease centres within ungulate winter ranges and consider adjusting estimates of habitat capability for deer based on our findings.
GENETIC ANALYSIS OF DNA REPLICATION IN BACTERIA: dna B MUTATIONS THAT SUPPRESS dnaC MUTATIONS AND dnaQ MUTATIONS THAT SUPPRESS dnaE MUTATIONS IN SALMONELLA TYPHIMURIUM
Authors: Maurer Russell , Osmond Barbara C , Botstein David 
Published: January 2021

ABSTRACT
We have isolated and characterized extragenic suppressors of mutations in two different target genes that affect DNA replication in Salmonella typhimurium. Both the target and the suppressor genes are functional homologues of known replication genes of E. coli that were identified in intergeneric complementation tests. Our results point to interactions in vivo involving the dnaB and dnaC proteins in one case and the dnaQ and dnaE proteins in the other case. The suppressor mutations, which were isolated as derivatives of λ-Salmonella in vitro recombinants, were detected by an adaptation of the red plaque complementation assay. This method was applicable even when the locus of suppressor mutations was not chosen in advance.
THE EFFECT OF NONHOMOLOGOUS DNA SEQUENCES ON INTERPLASMIDIC RECOMBINATION
Authors: Laban Abraham , Silberstein Zipora , Cohen Amikam 
Published: January 2021

ABSTRACT
The effect of nonhomologous DNA sequences at one or both sides of short genetic intervals on recombination within that interval was investigated, using an interplasmidic recombination system in Escherichia coli K-12. The recombining plasmids were derivatives of pBR322 and pACYC184, which share a 1330-nucleotide sequence that includes the tet gene. The genetic interval was defined by the HindIII and BamHI or Bam HI and the SalI restriction endonuclease sites of this gene. The substantial differences between recombination frequencies measured within intervals bracketed or bounded on one side by major nonhomologies suggests that, in this system, strand exchange is polar and is blocked by major nonhomologies. This conclusion is substantiated by results of three-factor crosses and by structural analysis of recombination products. Results of two-factor crosses in recA genetic background and structural analysis of recombination products suggest that strand exchange occurs in the absence of a functional recA gene. "Opening" a bracketed BamHI-SalI genetic interval of the tet gene, at the SalI site, by substituting a major insertion with a short deletion, results in an increase in recombination frequency, within this genetic interval, which is greater than expected on the basis of the ratio of the length of homology on the two sides of the SalI site. This observation suggests that a genetic element that may affect rate of recombination initiation, polarity of strand exchange, template specificity in mismatch repair or more than one of these events may be present on the outer side of the SalI site of the tet gene.
TEMPERATURE-SENSITIVE MUTATIONS OF THE NOTCH LOCUS IN DROSOPHILA MELANOGASTER
Authors: Shellenbarger David L , Mohler J Dawson 
Published: January 2021

ABSTRACT
Temperature-conditional mutations of the Notch locus were characterized in an attempt to understand the organization of a "complex locus" and the control of its function in development. Among 21 newly induced Notch alleles, about one-half are temperature-conditional for some effects, and three are temperature-sensitive for viability. One temperature-sensitive lethal, l(1)Nts1, is functionally non-complementing for all known effects of Notch locus mutations and maps at a single site within the locus. Among the existing alleles involved in complex patterns of interallelic complementation, Ax59d5 is found to be temperature-sensitive, while fag, spl, and l(1)N are temperature-independent. Whereas temperature-sensitive alleles map predominantly to the right-most fifth of the locus, fag, spl, and l(1)N are known to map to the left of this region. Temperature-shift experiments demonstrate that fag, spl, and l(1)N cause defects at specific, non-overlapping times in development.—We conclude (1) that the Notch locus is a single cistron (responsible for a single functional molecule, presumably a polypeptide); (2) that the right-most fifth of the locus is, at least in part, the region involved in coding for the Notch product; (3) that the complexity of interallelic complementation is a developmental effect of mutations that cause defects at selected times and spaces, and that complementation occurs because the mutant defects are temporally and spatially non-overlapping; and (4) that mutants express selected defects due to critical temporal and spatial differences in the chemical conditions controlling the synthesis or function of the Notch product. The complexity of the locus appears to reside in controlling the expression (synthesis or function) of the Notch product in development.
NEGATIVE COMPLEMENTATION AT THE NOTCH LOCUS OF DROSOPHILA MELANOGASTER
Authors: Foster Geoffrey G 
Published: January 2021

ABSTRACT
Four Abruptex alleles (AxE1, AxE 2, Ax9 B 2, and Ax16172) have been mapped within the Notch locus. Based on their visible phenotypes and their interactions with one another and with N mutations, the Ax alleles can be divided into two groups. Heterozygous combinations of members of the same group are intermediate in phenotype compared to the respective homozygotes, whereas heterozygotes of Ax alleles from different groups exhibit negative heterosis, being much less viable and more extremely mutant than either homozygote. It is suggested that the Notch locus is a multi-functional regulator ("integrator") gene, whose product possesses both "repressor" and "activator" functions for the processes it regulates.
The regulatory network for petal anthocyanin pigmentation is shaped by the MYB5a/NEGAN transcription factor in Mimulus
Authors: Zheng Xingyu , Om Kuenzang , Stanton Kimmy A , Thomas Daniel , Cheng Philip A , Eggert Allison , Simmons Emily , Yuan Yao-Wu , Conradi Smith Gregory D , Puzey Joshua R , Cooley Arielle M 
Published: January 2021

Abstract
Much of the visual diversity of angiosperms is due to the frequent evolution of novel pigmentation patterns in flowers. The gene network responsible for anthocyanin pigmentation, in particular, has become a model for investigating how genetic changes give rise to phenotypic innovation. In the monkeyflower genus Mimulus, an evolutionarily recent gain of petal lobe anthocyanin pigmentation in M. luteus var. variegatus was previously mapped to genomic region pla2. Here, we use sequence and expression analysis, followed by transgenic manipulation of gene expression, to identify MYB5a—orthologous to the NEGAN transcriptional activator from M. lewisii—as the gene responsible for the transition to anthocyanin-pigmented petals in M. l. variegatus. In other monkeyflower taxa, MYB5a/NEGAN is part of a reaction-diffusion network that produces semi-repeating spotting patterns, such as the array of spots in the nectar guides of both M. lewisii and M. guttatus. Its co-option for the evolution of an apparently non-patterned trait—the solid petal lobe pigmentation of M. l. variegatus—illustrates how reaction-diffusion can contribute to evolutionary novelty in non-obvious ways. Transcriptome sequencing of a MYB5a RNAi line of M. l. variegatus reveals that this genetically simple change, which we hypothesize to be a regulatory mutation in cis to MYB5a, has cascading effects on gene expression, not only on the enzyme-encoding genes traditionally thought of as the targets of MYB5a but also on all of its known partners in the anthocyanin regulatory network.
Psychological Pathways Linking Parent–Child Relationships to Objective and Subjective Sleep Among Older Adults
Authors: Wang Haowei , Kim Kyungmin , Burr Jeffrey A , Wu Bei 
Published: January 2021

Abstract

Objectives
This study investigated whether older adults with better relationships with their adult children have better subjective and objective sleep quality than older adults with less-positive relationships with their children. We also examined whether depressive symptoms and loneliness mediated the association between parent–child relationships and sleep among older adults.

Methods
Data were used from the second wave of the National Social life, Health, and Aging Project, in which 548 respondents (aged 62–90) participated in the sleep survey to measure their actigraph sleep activity for three consecutive days. Respondents also reported sleep quality (i.e., sleep duration and insomnia symptoms), contact frequency, and emotional closeness with their children.

Results
Results from structural equation modeling showed that greater emotional closeness with children was directly associated with better objective sleep characteristics (i.e., sleep fragmentation and amount of sleep). Also, more frequent contact with children was directly related to fewer insomnia symptoms among older adults. Moreover, emotional closeness with children was indirectly linked to insomnia symptoms via depressive symptoms among older adults.

Discussion
This study provided evidence for psychological pathways linking parent–child relationships and older parents’ subjective sleep. The findings have implications for health professionals and family counselors who help people with sleep problems and relationship difficulties.
Functional Limitations, Social Integration, and Daily Activities in Late Life
Authors: Fingerman Karen L , Ng Yee To , Huo Meng , Birditt Kira S , Charles Susan T , Zarit Steven 
Published: January 2021

Abstract

Objectives
Disability in late life has been associated with increases in receiving care and loss of autonomy. The Disablement Process Model suggests that physical impairments lead to functional limitations that contribute to disabilities in managing household, job, or other demands. Yet, we know surprisingly little about how functional limitations are related to activities throughout the day among community-dwelling adults or the possible moderating role of social integration on these associations.

Methods
Community-dwelling adults (N = 313) aged 65 and older completed a baseline interview assessing their functional limitations, social ties, and background characteristics. Over 5–6 days, they answered questions about daily activities and encounters with social partners every 3 h on handheld Android devices.

Results
Multilevel logistic models revealed that functional limitations are associated with an increased likelihood of activities associated with poor health (e.g., TV watching, medical appointments) and reduced likelihood of social activities, or physical activities, chores, or leaving the home. Most moderation analyses were not significant; family and friends did not mitigate associations between functional limitations and daily activities, with the exception of medical appointments. Individuals with functional limitations were more likely to attend medical appointments when with their social partners than when alone.

Discussion
This study provided a modest indication that functional limitations in community-dwelling older adults are associated with patterns of activity that may lead to further limitations, disability, or loss of autonomy. Findings warrant longitudinal follow-up to establish subsequent patterns of decline or stability.
A Typical Week With Mild Cognitive Impairment
Authors: Renn Brenna N , Wool Jenny L , Belza Basia 
Published: January 2021

Abstract

Background and Objectives
Identifying the experience of people with mild cognitive impairment (MCI) may help develop research agendas, interventions, and other supports to better match individuals’ needs. The purpose of this study was to explore the subjective experience of a “typical week” living with MCI to document (a) important activities, (b) barriers to usual activities, and (c) facilitators and supports.

Research Design and Methods
We conducted remote individual photo-elicitation interviews with 11 community-dwelling adults aged 55 years or older with MCI. Participants each provided 5–10 photographs of daily life taken over 1 week to facilitate a semistructured qualitative interview. Interview transcriptions were coded in Dedoose software and analyzed using thematic analysis.

Results
Participants shared photos and narratives highlighting the important activities in a typical week, in which physical activity, social engagement, spiritual and religious practice, hobbies, and cognitive stimulation were central. Many also shared disruptions to former routines and reduction of activities alongside increased use of new strategies and environmental supports (e.g., calendars, smartphones). Finally, emergent themes centered on disclosure of their diagnosis and reflections about the future.

Discussion and Implications
Participant-generated images aided data collection and facilitated discussion of sensitive topics with individuals with MCI. Such narratives may illustrate the needs and opportunities to promote well-being in individuals with MCI, including engagement in meaningful and health-promoting activities, assessing barriers to important daily activities, and considering supports that match the experience and needs of those with MCI.
An ecological response to ethno-nationalistic populism: grassroots environmental peacebuilding in south Asia
Authors: Huda Mirza Sadaqat 
Published: January 2021

Abstract
The geopolitical and geophysical realities of south Asia create a deep sense of paradox. On the one hand, territorial disputes and hyper-nationalism have resulted in some of the most militarized borders in the world. South Asia's international boundaries are subject to a range of conflicts, which includes high-level armed confrontations between India and Pakistan and low-level, yet deadly, incidents at the India–Bangladesh border. On the other hand, the region's ecology is inherently interlinked through shared rivers, glaciers and forests. South Asia's borders therefore present unique opportunities for environmental peacebuilding, as they are the epicentre of political conflicts as well as the source of transnational ecological connections. This article argues that grassroots processes of environmental peacebuilding can be used to build societal resistance to the rise of ethno-nationalistic populism in south Asia. Using interview data, the article tests concepts on pathways to environmental peacebuilding against underlying drivers of regional conflicts. The study suggests ways by which grassroots environmental initiatives on the Sundarbans forest between India and Bangladesh and the Thar desert between India and Pakistan can address the contemporary rise in nativist politics. The article contributes to existing literature by connecting theories on pathways to environmental peacebuilding to the ideational drivers of territorial and political conflicts. It adds to policy discussions by suggesting an ecological response to the contemporary rise of ethno-nationalistic populism in various regions of the world.
The past and future(s) of environmental peacebuilding
Authors: Ide Tobias , Bruch Carl , Carius Alexander , Conca Ken , Dabelko Geoffrey D , Matthew Richard , Weinthal Erika 
Published: January 2021

Abstract
Environmental peacebuilding is a rapidly growing field of research and practice at the intersection of environment, conflict, peace and security. Focusing on these linkages is crucial in a time when the environment is a core issue of international politics and the number of armed conflicts remains high. This article introduces a special issue with a particular emphasis on environmental opportunities for building and sustaining peace. We first detail the definitions, theoretical assumptions and intellectual background of environmental peacebuilding. The article then provides context for the special issue by briefly reviewing core findings and debates of the first two generations of environmental peacebuilding research. Finally, we identify knowledge gaps that should be addressed in the next generation of research, and to which the articles in this special issue contribute: bottom-up approaches, gender, conflict-sensitive programming, use of big data and frontier technology, and monitoring and evaluation.