bluuebunny/crossref_metadata_2025_split huggingface dataset:
Nanogold Functionalized With Lipoamide-isoDGR: A Simple, Robust and Versatile Nanosystem for αvβ3-Integrin Targeting
Authors: Sacchi Angelina , Gasparri Anna Maria , Monieri Matteo , Anderluzzi Giulia , Colombo Barbara , Gori Alessandro , Corti Angelo , Curnis Flavio 
Published: May 2021

Gold nanoparticles functionalized with isoDGR, a tripeptide motif that recognizes αvβ3 integrin overexpressed in tumor vessels, have been used as nano-vectors for the delivery of cytokines to tumors. Functionalization of nanogold with this peptide has been achieved by coating nanoparticles with a peptide-albumin conjugate consisting of heterogeneous molecules with a variable number of linkers and peptides. To reduce nanodrug heterogeneity we have designed, produced and preclinically evaluated a homogeneous and well-defined reagent for nanogold functionalization, consisting of a head-to-tail cyclized CGisoDGRG peptide (iso1) coupled via its thiol group to maleimide-PEG11-lipoamide (LPA). The resulting iso1-PEG11-LPA compound can react with nanogold via lipoamide to form a stable bond. In vitro studies have shown that iso1, after coupling to nanogold, maintains its capability to bind purified αvβ3 and αvβ3-expressing cells. Nanogold functionalized with this peptide can also be loaded with bioactive tumor necrosis factor-α (TNF) to form a bi-functional nanodrug that can be stored for three days at 37°C or &gt;1 year at low temperatures with no loss αvβ3-binding properties and TNF-cytolytic activity. Nanoparticles functionalized with both iso1 and TNF induced tumor eradication in WEHI-164 fibrosarcoma-bearing mice more efficiently than nanoparticles lacking the iso1 targeting moiety. These results suggest that iso1-PEG11-LPA is an efficient and well-defined reagent that can be used to produce robust and more homogeneous nano-vectors for the delivery of TNF and other cytokines to αvβ3 positive cells.
Singular Spectrum Analysis of the Total Electron Content Changes Prior to M ≥ 6.0 Earthquakes in the Chinese Mainland During 1998–2013
Authors: Chen Hongyan , Miao Miao , Chang Ying , Wang Qiao , Shen Xuhui , Hattori Katsumi , Han Peng 
Published: May 2021

Early studies have shown evidence of the seismo-ionospheric perturbations prior to large earthquakes. Due to dynamic complexity in the ionosphere, the identification of precursory ionospheric changes is quite challenging. In this study, we analyze the total electron content (TEC) in the global ionosphere map and investigate the TEC changes prior to M ≥ 6.0 earthquakes in the Chinese Mainland during 1998–2013 to identify possible seismo-ionospheric precursors. Singular spectrum analysis is applied to extract the trend and periodic variations including diurnal and semi-diurnal components, which are dominated by solar activities. The residual ΔTEC which is mainly composed of errors and possible perturbations induced by earthquakes and geomagnetic activities is further investigated, and the root-mean-square error is employed to detect anomalous changes. The F10.7 and Dst index is also used as criterion to rule out the anomalies when intense solar or geomagnetic activities occur. Our results are consistent with those of previous studies. It is confirmed that the negative anomalies are dominant 1–5 days before the earthquakes at the fixed point (35°N, 90°E) during 0600–1000 LT. The anomalies are more obvious near the epicenter area. The singular spectrum analysis method help to establish a more reliable variation background of TEC and thus may improve the identification of precursory ionospheric changes.
The Value of Pre-Ablative I-131 Scan for Clinical Management in Patients With Differentiated Thyroid Carcinoma
Authors: van der Boom Trynke , Zandee Wouter T. , Dekkers Claire C. J. , van der Horst-Schrivers Anouk N. A. , Jansen Liesbeth , Kruijff Schelto , Brouwers Adrienne H. , Links Thera P. 
Published: May 2021

BackgroundA diagnostic I-131 (Dx) scan is used to detect a thyroid remnant or metastases before treatment of differentiated thyroid cancer (DTC) with I-131. The aim of this study is to specify in which patients with DTC a Dx scan could have an additional value, by studying the effect of the Dx scan on clinical management.MethodsPatients with DTC, treated with I-131 after thyroidectomy were included in this retrospective cohort study. Twenty-four hours after administration of 37 MBq I-131 a whole body Dx scan and an uptake measurement at the original thyroid bed were performed. Outcomes of the Dx scan and the subsequent changes in clinical management, defined as additional surgery or adjustment of I-131 activity, were reported. Risk factors for a change in clinical management were identified with a binary logistic regression.ResultsIn 11 (4.2%) patients clinical management was changed, including additional surgery (n=5), lowering I-131 activity (n=5) or both (n=1). Risk factors for a change in clinical management were previous neck surgery (OR 5.9, 95% CI: 1.4-24.5), surgery in a non-tertiary center (OR 13.4, 95% CI: 2.8 – 63.8), TSH &lt;53.4 mU/L (OR 19.64, 95% CI: 4.94-78.13), thyroglobulin ≥50.0 ng/L (OR 7.4, 95% CI: 1.6-34.9) and free T4 ≥4.75 pmol/L (OR 156.8, 95% CI: 128.4-864.2)ConclusionThe Dx scan can potentially change clinical management before treatment with I-131, but the yield is low. A Dx-scan should only be considered for patients with a high pre-scan risk of a change in management, based on patient history and prior center-based surgical outcomes.
Liver Proteome Profile of Growth Restricted and Appropriately Grown Newborn Wistar Rats Associated With Maternal Undernutrition
Authors: Sarli Polyxeni-Maria , Manousopoulou Antigoni , Efthymiou Elias , Zouridis Andreas , Potiris Anastasios , Pervanidou Panagiota , Panoulis Konstantinos , Vlahos Nikolaos , Deligeoroglou Efthymios , Garbis Spiros D. , Eleftheriades Makarios 
Published: May 2021

BackgroundFetal growth restriction (FGR) has been associated with adverse perinatal outcomes and epigenetic modifications that impact gene expression leading to permanent changes of fetal metabolic pathways and thereby influence development of disease in childhood and adult life. In this study, we investigated the result of maternal food restriction on liver protein expression in Wistar male newborn pups.Materials &amp; MethodsTen (n = 10) timed pregnant Wistar rats on their 14th day of gestation were randomly assigned to either control (n = 4) or food restricted group (n = 6). The control group had ad libitum access to food. In the food restricted group, maternal diet was limited in a moderate fashion (50%) from day 15 of pregnancy until delivery. All rats delivered spontaneously on day 21 and newborn pups were immediately weighed. Pups born to normally nourished mothers were considered as controls, while pups born to food restricted mothers were subdivided into two groups, based on their birth weight: growth restricted (FGR) and appropriately grown (non-FGR). Rats were euthanized immediately after birth and liver tissues of 11 randomly selected male offspring (FGR n = 4, non-FGR n = 4, control n = 3) were collected and analyzed using quantitative proteomics.ResultsIn total 6,665 proteins were profiled. Of these, 451 and 751 were differentially expressed in FGR and non-FGR vs. control, respectively, whereas 229 proteins were commonly expressed. Bioinformatics analysis of the differentially expressed proteins (DEPs) in FGR vs. control revealed induction of the super-pathway of cholesterol biosynthesis and inhibition of thyroid hormone metabolism, fatty acid beta oxidation and apelin liver signaling pathway. Analysis of DEPs in non-FGR vs. control groups showed inhibition of thyroid hormone metabolism, fatty acid beta oxidation, and apelin liver signaling pathway.ConclusionThis study demonstrates the impact of prenatal food restriction on the proteomic liver profile of FGR and non-FGR offspring underlying the importance of both prenatal adversities and birth weight on liver-dependent postnatal disease.
REW-ISA V2: A Biclustering Method Fusing Homologous Information for Analyzing and Mining Epi-Transcriptome Data
Authors: Zhang Lin , Chen Shutao , Ma Jiani , Liu Zhaoyang , Liu Hui 
Published: May 2021

Background: Previous studies have shown that N6-methyladenosine (m6A) is related to many life processes and physiological and pathological phenomena. However, the specific regulatory mechanism of m6A sites at the systematic level is not clear. Therefore, mining the RNA co-methylation patterns in the epi-transcriptome data is expected to explain the specific regulation mechanism of m6A.Methods: Considering that the epi-transcriptome data contains homologous information (the genes corresponding to the m6A sites and the cell lines corresponding to the experimental conditions), rational use of this information will help reveal the regulatory mechanism of m6A. Therefore, based on the RNA expression weighted iterative signature algorithm (REW-ISA), we have fused homologous information and developed the REW-ISA V2 algorithm.Results: Then, REW-ISA V2 was applied in the MERIP-seq data to find potential local function blocks (LFBs), where sites are hyper-methylated simultaneously across the specific conditions. Finally, REW-ISA V2 obtained fifteen LFBs. Compared with the most advanced biclustering algorithm, the LFBs obtained by REW-ISA V2 have more significant biological significance. Further biological analysis showed that these LFBs were highly correlated with some signal pathways and m6A methyltransferase.Conclusion: REW-ISA V2 fuses homologous information to mine co-methylation patterns in the epi-transcriptome data, in which sites are co-methylated under specific conditions.
Roles of IL-25 in Type 2 Inflammation and Autoimmune Pathogenesis
Authors: Deng Chong , Peng Na , Tang Yuan , Yu Na , Wang Cuicui , Cai Xiaoyan , Zhang Lijun , Hu Dajun , Ciccia Francesco , Lu Liwei 
Published: May 2021

Interleukin-17E (IL-25) is a member of the IL-17 cytokine family that includes IL-17A to IL-17F. IL-17 family cytokines play a key role in host defense responses and inflammatory diseases. Compared with other IL-17 cytokine family members, IL-25 has relatively low sequence similarity to IL-17A and exhibits a distinct function from other IL-17 cytokines. IL-25 binds to its receptor composed of IL-17 receptor A (IL-17RA) and IL-17 receptor B (IL-17RB) for signal transduction. IL-25 has been implicated as a type 2 cytokine and can induce the production of IL-4, IL-5 and IL-13, which in turn inhibits the differentiation of T helper (Th) 17. In addition to its anti-inflammatory properties, IL-25 also exhibits a pro-inflammatory effect in the pathogenesis of Th17-dominated diseases. Here, we review recent advances in the roles of IL-25 in the pathogenesis of inflammation and autoimmune diseases.
Effects of the Invasion of Caulerpa cylindracea in a Cymodocea nodosa Meadow in the Northern Adriatic Sea
Authors: Najdek Mirjana , Korlević Marino , Paliaga Paolo , Markovski Marsej , Ivančić Ingrid , Iveša Ljiljana , Felja Igor , Herndl Gerhard J. 
Published: May 2021

Graphical AbstractEffects of the presence of the invasive macroalgae C. cylindracea in the seagrass meadow evidenced by substantial loss in below-ground biomass of C. nodosa and lowering of the redox transition depth in the sediment underlying the mixed settlement.
Spike in Rhino-Orbital-Cerebral Mucormycosis Cases Presenting to a Tertiary Care Center During the COVID-19 Pandemic
Authors: Fouad Yousef A. , Abdelaziz Tougan Taha , Askoura Anas , Saleh Mohamed Ibrahim , Mahmoud Mohammad S. , Ashour Doaa Maamoun , Ashour Manar Maamoun 
Published: May 2021

Objective: To determine if there was an increase in the rate of cases presenting with rhino-orbital-cerebral mucormycosis (ROCM) to a tertiary care center during the first wave of the coronavirus disease 2019 (COVID-19) pandemic and the characteristics of the presenting cases.Methods: Retrospective observational study reviewing ROCM cases presenting from March 25 until September 25, 2020. Cases fulfilling the clinical, radiological, and pathological/microbiological criteria for diagnosis with ROCM were included. The number of cases presenting during the designated interval, their COVID-19 status, comorbidities, and clinical presentation were analyzed. The number of cases during the corresponding interval in the previous 3 years was used as reference to detect if there was a recent spike.Results: Of the 12 ROCM cases identified, 5 had a concurrent positive reverse transcription PCR (RT-PCR) test result for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), 1 had a prior positive result, and 6 did not have concurrent nor prior positive test results. Nine of the 12 cases had poorly controlled diabetes mellitus, and 2 cases had a hematological malignancy. All cases had orbital invasion, and eight cases had cerebral invasion. The number of cases identified during the interval is much higher than the numbers presenting in the prior 3 years during equivalent intervals (range, one to two cases) than those reported in the literature in different settings in the pre-pandemic era.Conclusions: There is an increased rate of ROCM cases presenting to our center during the first wave of the COVID-19 pandemic. This is a preliminary report, and further studies are needed to corroborate the findings and explain possible underlying links.
Evaluation of Eight-Item Vancomycin Prescribing Confidence Questionnaire Among Junior Doctors
Authors: Liu Lu , Jiang Zhehan , Xie Ana , Wang Weimin 
Published: May 2021

Background: Assessing the preparedness of junior doctors to use vancomycin is important in medical education. Preparedness is typically evaluated by self-reported confidence surveys.Materials and Methods: An eight-item vancomycin prescribing confidence questionnaire was developed, piloted, and evaluated. The questionnaire responses were collected from 195 junior doctors and a series of statistical techniques, such as principal component analysis and confirmatory factor analysis, and were implemented to examine the validity and reliability.Results: The principal component analysis supported a one-factor structure, which was fed into a confirmatory factor analysis model resulting in a good fit [comparative fit index (CFI) = 0.99, Tucker–Lewis index (TLI) = 0.99, root mean square error of approximation (RMSEA) = 0.08, standardized root mean square residual (SRMR) = 0.04]. Ordinal-based α was 0.95, and various ωs were all above 0.93, indicating a high reliability level. The questionnaire responses were further proved to be robust to extreme response patterns via item response tree modeling. Jonckheere–Terpstra test results (z = 6.5237, p = 3.429e−11) showed that vancomycin prescribing confidence differed based on the experience in order (i.e., four ordinal independent groups: “≤10 times,” “11–20 times,” “21–30 times,” and “≥31 times”) and therefore provided external validity evidences for the questionnaire.Conclusions: The questionnaire is valid and reliable such that teaching hospitals can consider using it to assess junior doctors' vancomycin prescribing confidence. Further investigation of the questionnaire can point to the relationship between the prescribing confidence and the actual performance.
Prediction of Hemorrhagic Transformation After Ischemic Stroke: Development and Validation Study of a Novel Multi-biomarker Model
Authors: Liu Junfeng , Wang Yanan , Jin Yuxi , Guo Wen , Song Quhong , Wei Chenchen , Li Jing , Zhang Shanshan , Liu Ming 
Published: May 2021

Objectives: We aimed to develop and validate a novel multi-biomarker model for predicting hemorrhagic transformation (HT) risk after acute ischemic stroke (AIS).Methods: We prospectively included patients with AIS admitted within 24 h of stroke from January 1st 2016 to January 31st 2019. A panel of 17 circulating biomarkers was measured and analyzed in this cohort. We assessed the ability of individual circulating biomarkers and the combination of multiple biomarkers to predict any HT, symptomatic HT (sHT) and parenchymal hematoma (PH) after AIS. The strategy of multiple biomarkers in combination was then externally validated in an independent cohort of 288 Chinese patients.Results: A total of 1207 patients with AIS (727 males; mean age, 67.2 ± 13.9 years) were included as a derivation cohort, of whom 179 patients (14.8%) developed HT. The final multi-biomarker model included three biomarkers [platelets, neutrophil-to-lymphocyte ratios (NLR), and high-density lipoprotein (HDL)] from different pathways, showing a good performance for predicting HT in both the derivation cohort (c statistic = 0·64, 95% CI 0·60–0·69), and validation cohort (c statistic = 0·70, 95% CI 0·58–0·82). Adding these three biomarkers simultaneously to the basic model with conventional risk factors improved the ability of HT reclassification [net reclassification improvement (NRI) 65.6%, P &lt; 0.001], PH (NRI 64.7%, P &lt; 0.001), and sHT (NRI 71.3%, P &lt; 0.001).Conclusion: This easily applied multi-biomarker model had a good performance for predicting HT in both the derivation and external validation cohorts. Incorporation of biomarkers into clinical decision making may help to identify patients at high risk of HT after AIS and warrants further consideration.
The Role of Neuroinflammation in Post-traumatic Epilepsy
Authors: Sun Lei , Shan Wei , Yang Huajun , Liu Ru , Wu Jianping , Wang Qun 
Published: May 2021

Post-traumatic epilepsy (PTE) is one of the consequences after traumatic brain injury (TBI), which increases the morbidity and mortality of survivors. About 20% of patients with TBI will develop PTE, and at least one-third of them are resistant to conventional antiepileptic drugs (AEDs). Therefore, it is of utmost importance to explore the mechanisms underlying PTE from a new perspective. More recently, neuroinflammation has been proposed to play a significant role in epileptogenesis. This review focuses particularly on glial cells activation, peripheral leukocytes infiltration, inflammatory cytokines release and chronic neuroinflammation occurrence post-TBI. Although the immune response to TBI appears to be primarily pro-epileptogenic, further research is needed to clarify the causal relationships. A better understanding of how neuroinflammation contributes to the development of PTE is of vital importance. Novel prevention and treatment strategies based on the neuroinflammatory mechanisms underlying epileptogenesis are evidently needed.Search StrategySearch MeSH Terms in pubmed: “[“Epilepsy”(Mesh)] AND “Brain Injuries, Traumatic”[Mesh]”. Published in last 30 years. 160 results were founded. Full text available:145 results. Record screened manually related to Neuroinflammation and Post-traumatic epilepsy. Then finally 123 records were included.
Alteration of Vestibular Function in Pediatric Cochlear Implant Recipients
Authors: Koyama Hajime , Kashio Akinori , Fujimoto Chisato , Uranaka Tsukasa , Matsumoto Yu , Kamogashira Teru , Kinoshita Makoto , Iwasaki Shinichi , Yamasoba Tatsuya 
Published: May 2021

Background:Vestibular dysfunction is a complication of cochlear implantation (CI). Reports on the evaluation of vestibular function before and after CI are limited, especially in children. We investigated the effect of CI on vestibular function in pediatric patients.Patients and Methods:We routinely evaluated vestibular function before but not immediately after CI. Therefore, patients who underwent sequential bilateral CI were enrolled in this study. Seventy-three children who underwent sequential CI from 2003 to 2020 at our hospital were included. Since the vestibular function of the first implanted ear was evaluated before the second surgery for the contralateral ear, post-CI evaluation timing differed among the cases. The evaluation included a caloric test, a cervical vestibular-evoked myogenic potential (cVEMP) test, and a damped rotation test. The objective variables included the results of these tests, and the explanatory variables included the age at surgery, cause of hearing loss, electrode type, and surgical approach used. The associations of these tests were analyzed.Results:cVEMP was the most affected after CI (36.1%), followed by the caloric test (23.6%), and damped rotation test (7.8%). Cochleostomy was significantly more harmful than a round window (RW) approach or an extended RW approach based on the results of the caloric test (p= 0.035) and damped rotation test (p= 0.029). Perimodiolar electrodes affected the caloric test results greater than straight electrodes (p= 0.041). There were no significant associations among these tests' results.Conclusions:Minimally invasive surgery in children using a round window approach or an extended round window approach with straight electrodes is desirable to preserve vestibular function after CI.
Endovascular Treatment of Intracranial Aneurysms in Small Peripheral Vessel Segments—Efficacy and Intermediate Follow-Up Results of Flow Diversion With the Silk Vista Baby Low-Profile Flow Diverter
Authors: Schüngel Marie-Sophie , Quäschling Ulf , Weber Erik , Struck Manuel Florian , Maybaum Jens , Bailis Nikolaos , Arlt Felix , Richter Cindy , Hoffmann Karl-Titus , Scherlach Cordula , Schob Stefan 
Published: May 2021

Background and Purpose: Low-profile flow diverter stents (FDS) quite recently amended peripheral segments as targets for hemodynamic aneurysm treatment; however, reports on outcomes, especially later than 3 months, are scarce. This study therefore reports our experience with the novel silk vista baby (SVB) FDS and respective outcomes after 8 and 11 months with special respect to specific adverse events.Materials and Methods: Forty-four patients (mean age, 53 years) harboring 47 aneurysms treated with the SVB between June 2018 and December 2019 were included in our study. Clinical, procedural, and angiographic data were collected. Follow-ups were performed on average after 3, 8, and 11 months, respectively. Treatment effect was assessed using the O'Kelly Marotta (OKM) grading system.Results: Overall, angiographic follow-ups were available for 41 patients/45 aneurysms. Occlusion or significant reduction in aneurysmal perfusion (OKM: D1, B1–B3 and A2–A3) was observed in 98% of all aneurysms after 8 months. Only 2% of the treated aneurysms remained morphologically unaltered and without an apparent change in perfusion (OKM A1). Adverse events in the early post-interventional course occurred in seven patients; out of them, mRS-relevant morbidity at 90 days related to FDS treatment was observable in two patients. One death occurred in the context of severe SAH related to an acutely ruptured dissecting aneurysm of the vertebral artery.Conclusion: The SVB achieves sufficient occlusion rates of intracranial aneurysms originating from peripheral segments, which are comparable to prior established conventional FDS with acceptably low complication rates. However, alteration of a hemodynamic equilibrium in distal localizations requires special attention to prevent ischemic events.