bluuebunny/crossref_metadata_2025_split huggingface dataset:
Self-assembled graphene-based microfibers with eclectic optical properties
Authors: Ghamsari Mahdi , Madrakian Tayyebeh , Afkhami Abbas , Ahmadi Mazaher 
Published: March 2021

AbstractThe construction of graphene-based microfibers with reinforced mechanical and electrical properties has been the subject of numerous researches in recent years. However, the fabrication of graphene-based fibers with remarkable optical features still remains a challenge and has not been addressed so far. This paper aims to report a series of flexible self-assembled fibers, synthesized through a few-minute sonication of thermally oxidized graphene oxide nanosheets, so-called Nanoporous Over-Oxidized Graphene (NOG), in an acidic medium. These free-standing glassy fibers were classified into four distinct morphological structures and displayed a collection of intriguing optical properties comprising high transparency, strong birefringence, fixed body colorations (e.g. colorless, blue, green, and red), tunable interference marginal colorations, UV–visible-near IR fluorescence, and upconversion emissions. Moreover, they exhibited high chemical stability in strongly acidic, basic, and oxidizing media. The foregoing notable attributes introduce the NOG fiber as a promising candidate both for the construction of graphene-based photoluminescent textiles and the development of a wide variety of optical applications.
ATP signaling in the integrative neural center of Aplysia californica
Authors: Györi János , Kohn Andrea B. , Romanova Daria Y. , Moroz Leonid L. 
Published: March 2021

AbstractATP and its ionotropic P2X receptors are components of the most ancient signaling system. However, little is known about the distribution and function of purinergic transmission in invertebrates. Here, we cloned, expressed, and pharmacologically characterized the P2X receptors in the sea slug Aplysia californica—a prominent neuroscience model. AcP2X receptors were successfully expressed in Xenopus oocytes and displayed activation by ATP with two-phased kinetics and Na+-dependence. Pharmacologically, they were different from other P2X receptors. The ATP analog, Bz-ATP, was a less effective agonist than ATP, and PPADS was a more potent inhibitor of the AcP2X receptors than the suramin. AcP2X were uniquely expressed within the cerebral F-cluster, the multifunctional integrative neurosecretory center. AcP2X receptors were also detected in the chemosensory structures and the early cleavage stages. Therefore, in molluscs, rapid ATP-dependent signaling can be implicated both in development and diverse homeostatic functions. Furthermore, this study illuminates novel cellular and systemic features of P2X-type ligand-gated ion channels for deciphering the evolution of neurotransmitters.
A selective small-molecule inhibitor of c-Met suppresses keloid fibroblast growth in vitro and in a mouse model
Authors: Choi Min-Ha , Kim Jinhyun , Ha Jeong Hyun , Park Ji-Ung 
Published: March 2021

AbstractKeloids, tumor-like lesions that result from excessive scar formation, have no definitive treatment modality. Activation of c-mesenchymal-epithelial transition factor (c-Met) promotes cell proliferation and survival. Selective c-Met inhibitors, such as PHA-665752, may attenuate the activity of keloid fibroblasts and reduce keloid formation. Here, we aimed to evaluate the effect of PHA-665752, a second-generation selective small-molecule inhibitor of c-Met, on human keloid fibroblasts in vitro and in a mouse model. We performed in vitro cytotoxicity assays, scratch tests, western blotting, and immunofluorescence on human keloid fibroblasts. We also injected human fibroblasts into severe combined immunodeficient mice and measured the degree of nodule formation and skin histologic characteristics. We found that keloid fibroblast migration was inhibited by PHA-665752. Inhibitor treatment was also associated with lower expression of members of the hepatocyte growth factor/c-Met pathway, and lower fibroblast activity and collagen synthesis. In the in vivo experiments, PHA-665752—treated mice had lower nodule volumes and weights, accompanied by less inflammatory cell infiltration and collagen deposition, than those in control mice. These findings showed that although an in vivo model may not accurately represent the pathophysiology of human keloid development, PHA-665752 suppressed keloid fibroblast activity by inhibiting the c-Met—related tyrosine kinase pathway.
Effect of high proportion concentrate dietary on Yak jejunal structure, physiological function and protein composition during cold season
Authors: Jia Jianlei , Liang Chunnian , Wu Xiaoyun , Xiong Lin , Bao Pengjia , Chen Qian , Yan Ping 
Published: March 2021

AbstractThe current study aimed to investigate the damage of long-term high concentrate diet feeding pattern on Yak jejunal structure, physiological function and protein composition during cold season. Twelve Datong male Yak (Bos grunniens) with the same age from cold season were randomly selected and slaughtered to determine Yak jejunal digestive enzyme activity, morphology and protein composition on different feeding patterns in Tibetan Plateau. The results showed that Yak jejunum digestive enzyme activity and morphology of grazing reared group were better than those in the intensively reared group. A total of 96 differentially expressed proteins were identified by label-free Mass Spectrometry (MS), which could be concluded to two predominant themes: protein structure and inflammatory response. Nine differentially expressed proteins were correlated in Yak jejunum damage in different feeding patterns. According to this research, we found that feeding pattern resulted the differences in Yak jejunum physiological function, morphology and protein composition. This fact was confirmed long-term high dietary concentrate feeding could damage the jejunum epithelial morphology and function.
High S100A2 expression in keratinocytes in patients with drug eruption
Authors: Yoshioka Manabu , Sawada Yu , Saito-Sasaki Natsuko , Yoshioka Haruna , Hama Kayo , Omoto Daisuke , Ohmori Shun , Okada Etsuko , Nakamura Motonobu 
Published: March 2021

AbstractTelaprevir used as a protease inhibitor against hepatitis C virus is frequently associated with cutaneous adverse reactions. To explore a histological biomarker of cutaneous adverse events induced by telaprevir, we systematically searched for genes that were dysregulated by telaprevir in normal human epidermal keratinocytes (NHEKs). Microarray analysis and real-time polymerase chain reaction (PCR) revealed the significant increase in the expression of S100 calcium-binding protein A2 (S100A2) gene following treatment of NHEKs with telaprevir. Immunohistochemical analysis demonstrated that the expression of S100A2 was dominant in the spinous layer of the epidermis in patients with telaprevir-mediated severe-type drug eruptions and limited to the basal layer of the epidermis in healthy subjects. Furthermore, S100A2 expression increased after treatment with trichloroethylene and other medications, and the degree of S100A2 expression correlated with the severity of cutaneous adverse events. S100A2 expression also significantly increased in the skin of patients with atopic dermatitis and psoriasis. Taken together, S100A2 is highly expressed in the epidermis under inflammatory conditions and drug eruptions and may serve as a marker for keratinocyte damage in response to any inflammatory or toxic condition.
Tert-butylhydroquinone attenuates doxorubicin-induced dysregulation of testicular cytoprotective and steroidogenic genes, and improves spermatogenesis in rats
Authors: Ujah Godwin Adakole , Nna Victor Udo , Suleiman Joseph Bagi , Eleazu Chinedum , Nwokocha Chukwuemeka , Rebene Joy Assima , Imowo Michael Umana , Obi Emmanuel Ochui , Amachree Charlette , Udechukwu Evarest Chigozie , Mohamed Mahaneem 
Published: March 2021

AbstractDoxorubicin (DOX) is a broad-spectrum chemotherapeutic drug used in the treatment of cancers. It acts by generating reactive oxygen species in target cells. The actions are, however, not limited to cancerous cells as it attacks healthy cells, killing them. This study investigated the benefits of the antioxidant, tert-butylhydroquinone (tBHQ), on testicular toxicity following DOX therapy. Twenty-four adult male albino rats were assigned randomly into four groups (n = 6), namely: normal control (NC), tBHQ, DOX and tBHQ + DOX groups. tBHQ (50 mg/kg body weight in 1% DMSO) was administered orally for 14 consecutive days, while a single DOX dose (7 mg/kg body weight) was administered intraperitoneally on Day 8. DOX decreased sperm count, motility and viability, and decreased the levels of steroidogenesis-related proteins, and reproductive hormones. Furthermore, DOX decreased the expression of antioxidant cytoprotective genes, and decreased the protein level of proliferating cell nuclear antigen in the testis. Conversely, DOX increased the expression of pro-inflammatory and pro-apoptotic genes in the testis. These negative effects were ameliorated following the intervention with tBHQ. Our results suggest that tBHQ protects the testis and preserves both steroidogenesis and spermatogenesis in DOX-treated rats through the suppression of oxidative stress, inflammation and apoptosis.
Telomere lengths correlate with fitness but assortative mating by telomeres confers no benefit to fledgling recruitment
Authors: Young Rebecca C. , Kitaysky Alexander S. , Drummond Hugh M. 
Published: March 2021

AbstractAssortative mating by telomere lengths has been observed in several bird species, and in some cases may increase fitness of individuals. Here we examined the relationship between telomere lengths of Blue-footed Booby (Sula nebouxii) mates, long-lived colonial seabirds with high annual divorce rates. We tested the hypothesis that interactions between maternal and paternal telomere lengths affect offspring and parental survival. We found that relative telomere lengths (RTL) were strongly positively correlated between members of a breeding pair. In addition, RTL of both parents interacted to predict fledgling recruitment, although fledglings with two very long-RTL parents performed only averagely. Telomere lengths also predicted adult survival: birds with long telomeres were more likely to survive, but birds whose mate had long telomeres were less likely to survive. Thus, having long telomeres benefits survival, while choosing a mate with long telomeres benefits reproductive output while penalizing survival. These patterns demonstrate that while a breeder's RTL predicts offspring quality, assortative mating by RTL does not enhance fitness, and a trade-off between different components of fitness may govern patterns of assortative mating by telomere length. They also illustrate how testing the adaptive value of only one parent’s telomere length on either survival or reproductive success alone may provide equivocal results.
Physiological changes and transcriptome profiling in Saccharum spontaneum L. leaf under water stress and re-watering conditions
Authors: Li Changning , Wang Zhen , Nong Qian , Lin Li , Xie Jinlan , Mo Zhanghong , Huang Xing , Song Xiupeng , Malviya Mukesh Kumar , Solanki Manoj Kumar , Li Yangrui 
Published: March 2021

AbstractAs the polyploidy progenitor of modern sugarcane, Saccharum spontaneum is considered to be a valuable resistance source to various biotic and abiotic stresses. However, little has been reported on the mechanism of drought tolerance in S. spontaneum. Herein, the physiological changes of S. spontaneum GXS87-16 at three water-deficit levels (mild, moderate, and severe) and after re-watering during the elongation stage were investigated. RNA sequencing was utilized for global transcriptome profiling of GXS87-16 under severe drought and re-watered conditions. There were significant alterations in the physiological parameters of GXS87-16 in response to drought stress and then recovered differently after re-watering. A total of 1569 differentially expressed genes (DEGs) associated with water stress and re-watering were identified. Notably, the majority of the DEGs were induced by stress. GO functional annotations and KEGG pathway analysis assigned the DEGs to 47 GO categories and 93 pathway categories. The pathway categories were involved in various processes, such as RNA transport, mRNA surveillance, plant hormone signal transduction, and plant-pathogen interaction. The reliability of the RNA-seq results was confirmed by qRT-PCR. This study shed light on the regulatory processes of drought tolerance in S. spontaneum and identifies useful genes for genetic improvement of drought tolerance in sugarcane.
Sediment supply dampens the erosive effects of sea-level rise on reef islands
Authors: Tuck Megan E. , Ford Murray R. , Kench Paul S. , Masselink Gerd 
Published: March 2021

AbstractLarge uncertainty surrounds the future physical stability of low-lying coral reef islands due to a limited understanding of the geomorphic response of islands to changing environmental conditions. Physical and numerical modelling efforts have improved understanding of the modes and styles of island change in response to increasing wave and water level conditions. However, the impact of sediment supply on island morphodynamics has not been addressed and remains poorly understood. Here we present evidence from the first physical modelling experiments to explore the effect of storm-derived sediment supply on the geomorphic response of islands to changes in sea level and energetic wave conditions. Results demonstrate that a sediment supply has a substantial influence on island adjustments in response to sea-level rise, promoting the increase of the elevation of the island while dampening island migration and subaerial volume reduction. The implications of sediment supply are significant as it improves the potential of islands to offset the impacts of future flood events, increasing the future physical persistence of reef islands. Results emphasize the urgent need to incorporate the physical response of islands to both physical and ecological processes in future flood risk models.
Comparative analysis of contributions of wet deposition and photodegradation to the removal of atmospheric BaP by MFDCCA
Authors: Liu Chunqiong , Guo Yuanyuan , Shi Kai , Zhang Jiao , Wu Bo , Du Juan 
Published: March 2021

AbstractBenzo [a] pyrene (BaP) in the atmosphere possess great carcinogenic potential to human health, and the understanding of its scavenging mechanisms has attracted considerable attention. In this work, a new quantitative method is proposed to make a comparative analysis of the long-term contributions of wet deposition and photodegradation to BaP removal based on multi-fractal detrended cross-correlation analysis (MFDCCA). According to the precipitation and global solar radiation (GSR) observations from 1998 to 2016 for two urban sites (Central/Western District and TsuenWan) in Hong Kong, the wet deposition and photodegradation of BaP are analyzed. Using MFDCCA method, long-term cross-correlation between precipitation/GSR and BaP are investigated. Moreover, the differences of multifractal features in cross-correlations of precipitation-BaP and GSR-BaP system are analyzed. Strong long-term persistence is observed in the cross-correlations for precipitation-BaP system in a one-year cycle; while cross-correlations between GSR and BaP show weak persistence over the whole timescale. Based on the meteorology in Hong Kong, this difference has been discussed. Then, contributions of wet deposition and photodegradation to atmospheric BaP removal are quantified based on MFDCCA method, which are further compared between summer and winter. The comparative analysis suggests that wet deposition plays a more significant role in the removal of atmospheric BaP. Specifically, in summer, the contributions of wet deposition are twice as much as that of photodegradation for both two sites; while in winter, the contribution of photodegradation is a little higher than that of wet deposition to BaP removal. Meanwhile, for wet deposition, the contributions in summer are about ten times greater than that in winter; while for photodegradation, the difference in contributions between summer and winter are relatively smaller. Furthermore, based on sliding window technique, the temporal evolutions in the contributions of wet deposition/photodegradation to BaP removal have been presented for both two sites. On this basis, it is discovered that the comprehensive contributions of wet deposition and photodegradation peak in June, and reach their lowest levels in December for both two sites. Quantifying the contribution of meteorological factors to the removal of atmospheric BaP is help for understanding its geochemical cycle.
Inhibition of SENP2-mediated Akt deSUMOylation promotes cardiac regeneration via activating Akt pathway
Authors: Chen Yijin , Xu Tong , Li Mengsha , Li Chuling , Ma Yusheng , Chen Guojun , Sun Yili , Zheng Hao , Wu Guangkai , Liao Wangjun , Liao Yulin , Chen Yanmei , Bin Jianping 
Published: March 2021

Abstract
Post-translational modification (PTM) by small ubiquitin-like modifier (SUMO) is a key regulator of cell proliferation and can be readily reversed by a family of SUMO-specific proteases (SENPs), making SUMOylation an ideal regulatory mechanism for developing novel therapeutic strategies for promoting a cardiac regenerative response. However, the role of SUMOylation in cardiac regeneration remains unknown. In the present study, we assessed whether targeting protein kinase B (Akt) SUMOylation can promote cardiac regeneration. Quantitative PCR and Western blotting results showed that small ubiquitin-like modifier-specific protease 2 (SENP2) is up-regulated during postnatal heart development. SENP2 deficiency promoted P7 and adult cardiomyocyte (CM) dedifferentiation and proliferation both in vitro and in vivo. Mice with SENP2 deficiency exhibited improved cardiac function after MI due to CM proliferation and angiogenesis. Mechanistically, the loss of SENP2 up-regulated Akt SUMOylation levels and increased Akt kinase activity, leading to a decrease in GSK3β levels and subsequently promoting CM proliferation and angiogenesis. In summary, inhibition of SENP2-mediated Akt deSUMOylation promotes CM differentiation and proliferation by activating the Akt pathway. Our results provide new insights into the role of SUMOylation in cardiac regeneration.
Global asymptotic stability of a general biased min‐consensus protocol
Authors: Mo Yuanqiu , Yu Lanlin , Yu Changbin 
Published: March 2021

AbstractIn this paper, a general biased min‐consensus protocol for continuous‐time multi‐agent systems is presented. In this protocol, agents considered as sources maintain static states, while the state of each non‐source agent evolves using a general min‐consensus‐like function, which takes the states of its neighbours and a biased term as inputs. The properties of the general function have been identified so that the proposed protocol can achieve global asymptotic stability. Unlike conventional consensus protocols where a network of agents reach an agreement on certain quantities of interest, agents under the general biased min‐consensus protocol will converge to different states but can generate various design results pertaining to complex combinatorial optimization problems, such as the shortest path problem and its variants.
Pushing planets into an inner cavity by a resonant chain
Authors: Ataiee S. , Kley W. 
Published: March 2021

Context. The orbital distribution of exoplanets indicates an accumulation of super-Earth sized planets close to their host stars in compact systems. When an inward disc-driven migration scenario is assumed for their formation, these planets could have been stopped and might have been parked at an inner edge of the disc, or be pushed through the inner disc cavity by a resonant chain. This topic has not been properly and extensively studied.
Aims. Using numerical simulations, we investigate the possibility that the inner planets in a resonant chain can be pushed into the disc inner cavity by outer planets.
Methods. We performed hydrodynamical and N-body simulations of planetary systems embedded in their nascent disc. The inner edge of the disc was represented in two different ways, resembling either a dead zone inner edge (DZ) or a disc inner boundary (IB). The main difference lies in the steepness of the surface density profile. The innermost planet always has a mass of 10 MEarth, with additional outer planets of equal or higher mass.
Results. A steeper profile is able to stop a chain of planets more efficiently than a shallower profile. The final configurations in our DZ models are usually tighter than in their IB counterparts, and therefore more prone to instability. We derive analytical expressions for the stopping conditions based on power equilibrium, and show that the final eccentricities result from torque equilibrium. For planets in thinner discs, we found, for the first time, clear signs for over-stable librations in the hydrodynamical simulations, leading to very compact systems. We also found that the popular N-body simulations may overestimate the number of planets in the disc inner cavity.
Age-related Decline in Renal Function is Attenuated in Master Athletes
Authors: Rosa Thiago dos Santos , Corrêa Hugo Luca , Barbosa Lucas Pinheiro , Santos Patrick Anderson dos , Leite Patrício Lopes de Araújo , Aguiar Samuel Silva , Deus Lysleine Alves , Maciel Larissa Alves , Neves Rodrigo Vanerson Passos , Simoes Herbert Gustavo 
Published: March 2021

AbstractThis study analyzed the kidney function and biomarkers of health in lifelong-trained sprinters and endurance runners, and compared them to untrained aged-matched and young controls. Sixty-two men (21–66 yr.) were recruited and allocated as master athletes from sprints (n=25), master athletes from endurance events (n=8), untrained middle-aged (n=14) and young controls (n=15). Participants underwent anamnesis, anthropometric measures and blood sampling for biochemical analyses of klotho, FGF23 and estimated glomerular filtration rate. Master sprinters presented better kidney function in relation to endurance athletes and their untrained peers (P<0.0001). A number of biochemical variables were observed that negatively (i. e., GDF-15, TGF-Beta, IL-18) or positively (i. e., klotho/FGF23 ratio and sestrin-2) correlated with eGFR. Sestrin-2 presented the strongest association with eGFR (r=0.5, P=0.03). Results also revealed that lifelong-trained individuals presented the highest probability of having better values for cystatin C and thus an estimated glomerular filtration rate that was 37–49% higher than untrained peers. Master sprinters presented better kidney function in relation to endurance athletes and middle-aged untrained peers. Sestrin-2 may play a role in exercise-induced kidney function protection.
Polycystic Ovary Syndrome and Fibrocystic Breast Disease: An Updated Review
Authors: Kunicki Michał , Smolarczyk Roman 
Published: March 2021

AbstractPolycystic ovary syndrome (PCOS) is the most common hormonal disorder in women of reproductive age. There is no clear association between PCOS and benign breast disease (BBD). The latter is a frequent benign disorder, affecting women between 20 and 50 years of age. To date, the classification remains controversial, and the risk of developing breast cancer that is associated with these changes is different depending on the histopathological findings. The most frequent changes are breast cysts, which are noted in up to 50% of patients older than 30 years of age. This up-to-date review presents the relationship between PCOS and BBD. In conclusion, there is no clear association between benign breast disease and PCOS. Further studies on a large population with prospectively collected data using updated PCOS criteria are necessary.