bluuebunny/crossref_metadata_2025_split huggingface dataset:
Methods of application of ozone therapy for the treatment of peri-implantation mucositis
Authors: Makedonova Iu. A. , Mikhalchenko D. V. , Afanaseva O. Yu. , Stavskaya S. V. , Dyachenko D. Yu. , Venskel E. V. , Salyamov H. Yu. 
Published: March 2021

At the dental reception, complications after dental implantation in the form of mucositis and peri-implantitis are becoming more and more common. There are quite a lot of risk factors for the development of inflammation of the parotid tissue, there is no single idea about the etiopathogenesis of the above pathology. Mucositis refers to the initial stage of peri-implantitis development and is reversible. The specialist should promptly diagnose the development of the inflammatory potential and start treatment, in order to prevent the development of bone destruction around the implants. One of the methods of local exposure to the lesion is ozone therapy.Goal. This paper describes the method of treatment of peri-implantation mucositis by ozone therapy.Materials and methods. In patients with peri-implantation mucositis, the inflammatory peri-implantation tissue was ozonated directly in the oral cavity with the Ozotron device in order to relieve inflammation and improve microcirculation in the oral cavity. Before performing oral ozonation, all patients underwent a general clinical examination to identify contraindications to this method. It also describes in detail the scheme that prevents the penetration of ozone into the body and prevents its ingestion.Results. During ozonation, a positive trend was obtained in the treatment of post-prosthetic complications during dental implantation. The developed scheme of ozonation in the oral cavity stops the inflammation of the peri-implantation tissue. Side effects and complications during ozone therapy directly in the oral cavity were not detected.Conclusions. The inclusion of ozone therapy in the treatment regimen of patients with peri-implantation mucositis is an appropriate and justified physiotherapy method.
Profiling the Production of Antimicrobial Secondary Metabolites by Xenorhabdus khoisanae J194 Under Different Culturing Conditions
Authors: Booysen Elzaan , Rautenbach Marina , Stander Marietjie A. , Dicks Leon M. T. 
Published: March 2021

Species from the genus Xenorhabdus, endosymbiotic bacteria of Steinernema nematodes, produce several antibacterial and antifungal compounds, some of which are anti-parasitic. In this study, we report on the effect growth conditions have on the production of antimicrobial compounds produced by Xenorhabdus khoisanae J194. The strain was cultured in aerated and non-aerated broth, respectively, and on solid media. Production of antimicrobial compounds was detected after 24 h of growth in liquid media, with highest levels recorded after 96 h. Highest antimicrobial activity was obtained from cells cultured on solid media. By using ultraperformance liquid chromatography linked to mass spectrometry and HPLC, a plethora of known Xenorhabdus compounds were identified. These compounds are the PAX lipopeptides (PAX 1′, PAX 3′, PAX 5, and PAX 7E), xenocoumacins and xenoamicins. Differences observed in the MS-MS fractionation patterns collected in this study, when compared to previous studies indicated that this strain produces novel xenoamicins. Three novel antimicrobial compounds, khoicin, xenopep and rhabdin, were identified and structurally characterized based on MS-MS fractionation patterns, amino acid analysis and whole genome analysis. The various compounds produced under the three different conditions indicates that the secondary metabolism of X. khoisanae J194 may be regulated by oxygen, water activity or both. Based on these findings X. khoisanae J194 produce a variety of antimicrobial compounds that may have application in disease control.
Persistent Cigarette Smoking Attenuates Plaque Stabilization in Response to Lipid-Lowering Therapy: A Serial Optical Coherence Tomography Study
Authors: Zhang Xiling , Peng Xiang , Li Lulu , Yu Huai , Yu Bo 
Published: March 2021

Objective: This study aimed to investigate the effect of smoking on morphological changes in non-culprit plaques in acute coronary syndrome (ACS) patients at 1 year after percutaneous coronary intervention (PCI), using optical coherence tomography (OCT).Background: Cigarette smoking is an important risk factor for coronary artery disease. However, the reasons for the high risk of re-infarction and worsened health among patients who continue to smoke after PCI remain unclear.Methods: A total of 129 non-culprit plaques were identified from 97 ACS patients who underwent OCT imaging at the time of PCI and at 1-year follow-up. Patients were divided into the following three groups according to their smoking status at 1-year follow-up: persistent smoking group (n = 26), smoking cessation group (n = 29), and nonsmoking group (n = 42). Medical history, serum cholesterol level, coronary angiography data, and OCT-determined plaque morphology were analyzed among the three groups.Results: Relative to baseline levels, the total cholesterol and low-density lipoprotein cholesterol levels significantly decreased in all three groups at 1-year follow-up after statin therapy (p &lt; 0.05). The persistent smoking group had a relatively smaller fibrous cap thickness (FCT) and a higher incidence of thin-cap fibroatheroma (TCFA) than the other two groups at 1-year follow-up (p &lt; 0.05), although the FCT increased and the incidence of TCFA decreased in all three groups.Conclusions: Persistent smoking is associated with an attenuated effect of statin therapy on plaque stabilization in ACS patients.
Immobilization of Iron Minerals on a Layered Silicate for Enhancing its Solar Photocatalytic Activity toward H2 Production
Authors: El-Hosainy Hamza , Tahawy Rafat , Esmat Mohamed , El-Kemary Maged , Ide Yusuke 
Published: March 2021

The development of efficient and cost-effective solar photocatalysts capable of producing hydrogen from formic acid as a hydrogen storage medium is still a challenging issue. Herein, we report that iron minerals, ferric iron hydroxy sulfates (FHS), immobilized on a natural layered silicate, magadiite, can be used as a photocatalyst to produce hydrogen from formic acid under irradiation with solar simulator. The material exhibits the hydrogen production rate of 470 μmol g−1 h−1, which is considerably higher than that obtained on other iron minerals and comparable to that obtained on precious metal-based photocatalyst ever reported. The present result may open a way to design efficient photocatalyst for hydrogen production from formic acid in an economically and environmentally friendly way.
Elevated GAS2L3 Expression Correlates With Poor Prognosis in Patients With Glioma: A Study Based on Bioinformatics and Immunohistochemical Analysis
Authors: Zhou Yan , Zhang Limin , Song Sirong , Xu Lixia , Yan Yan , Wu Haiyang , Tong Xiaoguang , Yan Hua 
Published: March 2021

BackgroundGrowth arrest–specific 2 like 3 (GAS2L3) is a cytoskeleton-associated protein that interacts with actin filaments and tubulin. Abnormal GAS2L3 expression has been reported to be associated with carcinogenesis. However, the biological role of GAS2L3 in glioma remains to be determined.MethodsThe transcriptome level of GAS2L3 and its relationship with clinicopathological characteristics were analyzed among multiple public databases and clinical specimens. Bioinformatics analyses were conducted to explore biological functions and prognostic value of GAS2L3 in glioma.ResultsGAS2L3 was substantially expressed in glioma, and high GAS2L3 expression correlated with shorter overall survival time and poor clinical variables. Gene set enrichment analysis (GSEA), single-sample gene-set enrichment analysis, and CIBERSORT algorithm analyses showed that GAS2L3 expression was closely linked to immune-related pathways, inflammatory activities, and immune cell infiltration. Moreover, GAS2L3 was synergistic with T cell–inflamed gene signature, immune checkpoints, T-cell receptor diversities, and neoantigen numbers.ConclusionThis study suggests that GAS2L3 is a prognostic biomarker for glioma, providing a reference for further study of the potential role of GAS2L3 in the immunomodulation of glioma.
Chest CT Images for COVID-19: Radiologists and Computer-Based Detection
Authors: Dou Qingli , Liu Jiangping , Zhang Wenwu , Gu Yanan , Hsu Wan-Ting , Ho Kuan-Ching , Tong Hoi Sin , Yu Wing Yan , Lee Chien-Chang 
Published: March 2021

BackgroundCharacteristic chest computed tomography (CT) manifestation of 2019 novel coronavirus (COVID-19) was added as a diagnostic criterion in the Chinese National COVID-19 management guideline. Whether the characteristic findings of Chest CT could differentiate confirmed COVID-19 cases from other positive nucleic acid test (NAT)-negative patients has not been rigorously evaluated.PurposeWe aim to test whether chest CT manifestation of 2019 novel coronavirus (COVID-19) can be differentiated by a radiologist or a computer-based CT image analysis system.MethodsWe conducted a retrospective case-control study that included 52 laboratory-confirmed COVID-19 patients and 80 non-COVID-19 viral pneumonia patients between 20 December, 2019 and 10 February, 2020. The chest CT images were evaluated by radiologists in a double blind fashion. A computer-based image analysis system (uAI System, Lianying Inc., Shanghai, China) detected the lesions in 18 lung segments defined by Boyden classification system and calculated the infected volume in each segment. The number and volume of lesions detected by radiologist and computer system was compared with Chi-square test or Mann-Whitney U test as appropriate.ResultsThe main CT manifestations of COVID-19 were multi-lobar/segmental peripheral ground-glass opacities and patchy air space infiltrates. The case and control groups were similar in demographics, comorbidity, and clinical manifestations. There was no significant difference in eight radiologist identified CT image features between the two groups of patients. There was also no difference in the absolute and relative volume of infected regions in each lung segment.ConclusionWe documented the non-differentiating nature of initial chest CT image between COVID-19 and other viral pneumonia with suspected symptoms. Our results do not support CT findings replacing microbiological diagnosis as a critical criterion for COVID-19 diagnosis. Our findings may prompt re-evaluation of isolated patients without laboratory confirmation.
Enhanced Written vs. Verbal Recall Accuracy Associated With Greater Prefrontal Activation: A Near-Infrared Spectroscopy Study
Authors: Zhang Jianan , Wang Ya , Zhang Yu , Li Brian , Zhang Yi 
Published: March 2021

Background: Memory efficiency is influenced by the modalities of acquisition and retrieval. The recall accuracy of read or voiced material differs depending on whether the recall is given verbally or in writing. The medial prefrontal cortex (mPFC) is critical for both attentional allocation and short-term memory, suggesting that different short-term memory recall modalities are associated with distinct mPFC processes and activation patterns.Methods: Near-infrared spectroscopy (NIRS) was used to monitor mPFC oxygenation parameters of 30 healthy subjects during acquisition and recall tasks as a measure of neural activity. Oxygenation parameters and recall accuracy were compared between oral and written answers and the potential correlations were analyzed.Results: Written responses were more accurate than verbal responses to the same questions and evoked greater changes in mPFC oxyhemoglobin (oxyHb) and total Hb (total-Hb). Furthermore, there were significant positive correlations between recall accuracy and both Δ[oxyHb] and Δ[total-Hb] in the mPFC.Conclusion: Memory accuracy of written material is greater when responses are also written rather than verbal. In both cases, recall accuracy was correlated with the degree of mPFC activity. This NIRS-based learning and memory paradigm may be useful for monitoring training efficacy, such as in patients with cognitive impairment.
Case Report: Successful Cerebral Revascularization and Cardiac Transplant in a 16-Year-Old Male With Syndromic BRCC3-Related Moyamoya Angiopathy
Authors: Pyra Pierrick , Darcourt Jean , Aubert-Mucca Marion , Brandicourt Pierre , Patat Olivier , Cheuret Emmanuel , Brochard Karine , Sevely Annick , Calviere Lionel , Karsenty Clément 
Published: March 2021

Background:BRCC3/MTCP1 deletions are associated with a rare familial moyamoya angiopathy with extracranial manifestations.Case: We report the case of an adolescent male presenting with progressive and symptomatic moyamoya angiopathy and severe dilated cardiomyopathy caused by a hemizygous deletion of BRCC3/MTCP1. He was treated for renovascular hypertension by left kidney homograft and right nephrectomy in infancy and had other syndromic features, including cryptorchidism, growth hormone deficiency, and facial dysmorphism. Due to worsening of the neurological and cardiac condition, he was treated by a direct superficial temporal artery to middle cerebral artery bypass to enable successful cardiac transplant without cerebral damage.Conclusions:BRCC3-related moyamoya is a devastating disease with severe heart and brain complications. This case shows that aggressive management with cerebral revascularization to allow cardiac transplant is feasible and efficient despite end-stage heart failure.
Autophagic Heterogeneity in Gastric Adenocarcinoma
Authors: Yoon Ju-Yoon , Brezden-Masley Christine , Streutker Catherine J. 
Published: March 2021

Background and AimGastric/gastroesophageal junction (GEJ) adenocarcinoma is a heterogeneous disease, with various etiologies and with tumors encompassing a spectrum of histologic and molecular subtypes. “Autophagy” includes two related but distinct homeostatic processes that promote cell survival under adverse conditions, namely macro- and chaperone-mediated autophagy. There is increasing evidence of the roles autophagy may play in tumorigenesis.MethodsAutophagic pathways were examined in the context of the heterogeneity intrinsic to gastric/GEJ adenocarcinoma, utilizing immunohistochemistry targeting specific proteins within the pathways (p62, LAMP2A, LC3B). We examined whole sections of normal and dysplastic gastric mucosa, as well as a tissue microarray of adenocarcinomas.ResultsDysplastic gastric epithelium was marked by frequent nuclear p62 and aberrant LAMP2A expression compared to normal. Examining the pattern of LC3B/cytoplasmic p62 immuno-reactivity in gastric adenocarcinoma demonstrated a predominant pattern of LC3BHigh/p62High staining (56/86, 65.1%), which has been previously associated with active, but impaired macroautophagy. There were no statistically significant associations seen between LC3B/cytoplasmic p62 staining patterns with tumor grade, histotype, or approximated TCGA molecular subtype. LAMP2A and nuclear p62 and staining patterns were also heterogeneous across the cohort, but with no statistically significant associations seen. The prognostic significance of the three proteins was limited, however high nuclear p62 levels were associated with worse overall survival (log-rank p-value = 0.0396).ConclusionOur data demonstrate the dynamic nature of autophagic proteins in the gastric epithelium, and we expand the biological heterogeneity observed in gastric/GEJ adenocarcinoma to include autophagy.
Role of Hyperbaric Oxygenation Plus Hypofractionated Stereotactic Radiotherapy in Recurrent High-Grade Glioma
Authors: Arpa Donatella , Parisi Elisabetta , Ghigi Giulia , Cortesi Annalisa , Longobardi Pasquale , Cenni Patrizia , Pieri Martina , Tontini Luca , Neri Elisa , Micheletti Simona , Ghetti Francesca , Monti Manuela , Foca Flavia , Tesei Anna , Arienti Chiara , Sarnelli Anna , Martinelli Giovanni , Romeo Antonio 
Published: March 2021

BackgroundThe presence of hypoxic cells in high-grade glioma (HGG) is one of major reasons for failure of local tumour control with radiotherapy (RT). The use of hyperbaric oxygen therapy (HBO) could help to overcome the problem of oxygen deficiency in poorly oxygenated regions of the tumour. We propose an innovative approach to improve the efficacy of hypofractionated stereotactic radiotherapy (HSRT) after HBO (HBO-RT) for the treatment of recurrent HGG (rHGG) and herein report the results of an ad interim analysis.MethodsWe enrolled a preliminary cohort of 9 adult patients (aged &gt;18 years) with a diagnosis of rHGG. HSRT was administered in daily 5-Gy fractions for 3-5 consecutive days a week. Each fraction was delivered up to maximum of 60 minutes after HBO.ResultsMedian follow-up from re-irradiation was 11.6 months (range: 3.2-11.6 months). The disease control rate (DCR) 3 months after HBO-RT was 55.5% (5 patients). Median progression-free survival (mPFS) for all patients was 5.2 months (95%CI: 1.34-NE), while 3-month and 6-month PFS was 55.5% (95%CI: 20.4-80.4) and 27.7% (95%CI: 4.4-59.1), respectively. Median overall survival (mOS) of HBO-RT was 10.7 months (95% CI: 7.7-NE). No acute or late neurologic toxicity &gt;grade (G)2 was observed in 88.88% of patients. One patient developed G3 radionecrosis.ConclusionsHSRT delivered after HBO appears to be effective for the treatment of rHGG, it could represent an alternative, with low toxicity, to systemic therapies for patients who cannot or refuse to undergo such treatments.Clinical Trial Registrationwww.ClinicalTrials.gov, identifier NCT 03411408.
Mild Cognitive Impairment in de novo Parkinson's Disease: Selective Attention Deficit as Early Sign of Neurocognitive Decay
Authors: Cammisuli Davide Maria , Pagni Cristina , Palermo Giovanni , Frosini Daniela , Bonaccorsi Joyce , Radicchi Claudia , Cintoli Simona , Tommasini Luca , Tognoni Gloria , Ceravolo Roberto , Bonuccelli Ubaldo 
Published: March 2021

Background: In the present study, we aimed to better investigate attention system profile of Parkinson's disease-Mild Cognitive Impairment (PD-MCI) patients and to determine if specific attentional deficits are associated with 123I-FP-CIT SPECT.Methods: A total of 44 de novo drug-naïve PD patients [(27) with normal cognition (PD-NC) and 17 with MCI (PD-MCI)], 23 MCI patients and 23 individuals with subjective cognitive impairment (SCI) were recruited at the Clinical Neurology Unit of Santa Chiara hospital (Pisa University Medical School, Italy). They were assessed by a wide neuropsychological battery, including Visual Search Test (VST) measuring selective attention. Performances among groups were compared by non-parametric tests (i.e., Kruskal-Wallis and Mann-Whitney, Bonferroni corrected). Further, Spearman's rank correlations were performed to explore the association between neuropsychological variables and 123I-FP-CIT SPECT data in PD subgroup.Results: PD-MCI patients performed worse on VST than patients with PD-NC (p = 0.002), patients with MCI and individuals with SCI (p &lt; 0.001). The performance of PD-MCI patients on VST significantly correlated with caudate nucleus 123I-FP-CIT SPECT uptake (rho = 0.582, p &lt; 0.05), whereas a negative correlation between such test and 123I-FP-CIT SPECT uptake in the left putamen (rho = −0.529, p &lt; 0.05) was found in PD-NC patients.Conclusions: We suggest that selective attention deficit might be a trigger of cognitive decay in de novo PD-MCI patients. The VST should be routinely used to detect attentional deficits in hospital clinical practice, in the light of its closely association with dopamine depletion of basal ganglia in mildly impaired PD patients.
ANALISIS KESTABILAN SISTEM DINAMIK PARTIKEL DALAM MEDAN POTENSIAL HARDCORE DOUBLE YUKAWA
Authors: Lalus Herry F. , Yusuf Yusniati H. Muh. 
Published: March 2021

Abstrak
Paper ini mengkaji sistem dinamik partikel yang bergerak dalam pengaruh medan potensial Hardcore Double Yukawa (HCDY) [1]. Kajian ini difokuskan pada analisis kondisi stabil sistem dinamik dengan memanfaatkan sifat kestabilan linear. Massa partikel dalam sistem ini ditinjau sebagai fungsi koordinat, sehingga berbentuk lebih umum. Potensial HCDY digunakan dalam mengkonstruksi Hamiltonian sistem dan selanjutnya dipakai untuk menentukan matriks Jacobinya. Selanjutnya, nilai eigen matriks menjadi dasar menganalisis kestabilan sistem dinamik ini. Untuk sistem dengan potensial jenis ini, hanya dimungkinkan dua jenis kestabilan, yaitu saddle dan rotor. Syarat-syarat untuk kedua jenis kestabilan ini juga ditampilkan.
Kata kunci: potensial Hard Core Double Yukawa; sistem dinamik; keadaan stabil
Abstract This paper discusses the dynamical sistem of particle that move under the influence of the Hard Core Double Yukawa (HCDY) potential field[1]. This study focuses on analyzing the stable condition of the dynamical sistem by utilizing the nature of linear stability. The mass of particle in this system is depend on coordinate function, so its form is more general. The HCDY potential is used in constructing the Hamiltonian system and then used to determine the Jacobian matrix.Futhermore, the matrix eigenvalue is the basis for analyzing the stability of this dyanamical system. For systems with this type of potential, only two types of stability are possible, namely saddle and rotor. The conditions for both types of stability are also displayed.
Keywords: Hard Core Double Yukawa potential; dynamical system; stability