bluuebunny/crossref_metadata_2025_split huggingface dataset:
Open hardware microsecond dispersive transient absorption spectrometer for linear optical response
Authors: Hutchison Christopher D. M. , Parker Susan , Chukhutsina Volha , van Thor Jasper J. 
Published: November 2021

Abstract
An open hardware design and implementation for a transient absorption spectrometer are presented that has microsecond time resolution and measures full difference spectra in the visible spectral region from 380 to 750 nm. The instrument has been designed to allow transient absorption spectroscopy measurements of either low or high quantum yield processes by combining intense sub-microsecond excitation flashes using a xenon lamp together with stroboscopic non-actinic white light probing using LED sources driven under high pulsed current from a capacitor bank. The instrument is sensitive to resolve 0.15 mOD flash-induced differences within 1000 measurements at 20 Hz repetition rate using an inexpensive CCD sensor with 200 μm pixel dimension, 40 K electrons full well capacity and a dynamic range of 1800. The excitation flash has 230 ns pulse duration and the 2 mJ flash energy allows spectral filtering while retaining high power density with focussing to generate mOD signals in the 10–4–10–1 ΔOD range. We present the full electronics design and construction of the flash and probe sources, the optics as well as the timing electronics and CCD spectrometer operation and modification for internal signal referencing. The performance characterisation and example measurements are demonstrated using microsecond TAS of Congo red dye, as an example of a low quantum yield photoreaction at 2% with up to 78% of molecules excited. The instrument is fully open hardware and combines inexpensive selection of commercial components, optics and electronics and allows linear response measurements of photoinduced reactions for the purpose of accurate global analysis of chemical dynamics.

Graphical abstract
Hemodynamic and Anatomical Factors in Arteriovenous Malformation Clinical Presentation: 45 Case Studies
Authors: Chalil Alan , Raupp Eduardo F. , Duckwiler Gary R. , Viñuela Fernando , Lownie Stephen P. 
Published: November 2021

Abstract:Background:Hemodynamic factors have been implicated in hemorrhage from cerebral arteriovenous malformations (AVMs). The goal of this endovascular study is to analyze the hemodynamic variability in AVM feeders in a balanced group of ruptured and unruptured AVMs of various sizes and at both superficial and deep locations.Methods:We monitored feeder artery pressure (FP) using microcatheters in 45 patients with AVMs (16 with hemorrhage, 29 without) during superselective angiography and AVM embolization.Results:Mean FP was 49 mm Hg. Significant determinants of FP were the systemic pressure (p < 0.001), AVM size (p = 0.03), and the distance of the microcatheter tip from the Circle of Willis (p = 0.06), but not the presence of hemorrhage, patient age, or feeder artery diameter. The FP in ruptured AVMs was 7 mm Hg higher than in unruptured ones (53.8 mm Hg vs. 47.1 mm Hg, p = 0.032). The presence or absence of venous outflow stenosis and the position of the AVM nidus (superficial or deep to the cortical surface) were important anatomical predictors of AVM presentation.Conclusion:The pressure in the feeding artery supplying an AVM is the result of factors which include the systemic arterial pressure, the size of the AVM nidus, and the distance of the AVM from the Circle of Willis. The correlation between these variables makes it difficult to study the risk of hemorrhage as a function of a single factor, which may account for the variation in the conclusions of previous studies.
BAROQUE AROUND THE CLOCK: DANIELLO BARTOLI SJ (1608–1685) AND THE USES OF GLOBAL HISTORY
Authors: Ditchfield Simon 
Published: November 2021

ABSTRACTRight from its foundation in 1540, the Society of Jesus recognised the value and role of visual description (ekphrasis) in the persuasive rhetoric of Jesuit missionary accounts. Over a century later, when Jesuit missions were to be found on all the inhabited continents of the world then known to Europeans, descriptions of the new-found lands were being read for the entertainment as well as the edification of their Old World audiences. The first official history of the Society's missions in the vernacular, the volumes authored by Daniello Bartoli (1608–1685), played an important role in communicating a sense of the distinctiveness of the order's global mission. Referred to by Giacomo Leopardi (1798–1837) as the ‘Dante of baroque prose’, Bartoli developed a particularly variegated and intensely visual idiom to meet the challenge of describing parts of the world which the majority of his readers, including himself, would never visit.
Progressing the development of a food literacy questionnaire using cognitive interviews
Authors: Thompson Courtney , Adams Jean , Vidgen Helen Anna 
Published: November 2021

AbstractObjective:Food literacy is the knowledge, skills and behaviours needed to meet food needs and determine intake and is conceptualised as eleven components under four domains of planning and managing, selecting, preparing, and eating. Previous measures of food literacy vary in their adherence to the conceptualisation and ability to capture totality of eating. This study aimed to determine items for inclusion and exclusion in a food literacy item pool and capture the general public’s interpretation of everyday food literacy practices.Design:Beginning with an item pool from previous studies, cognitive interviews were conducted using think-aloud and verbal probing methods. Data were first analysed for applicability, clarity, ambiguity and logic, then for emergent themes to ensure items captured the totality of the participant’s eating.Setting:AustraliaParticipants:Australian residents over 18 years of age recruited via Facebook residential groups (n 20).Results:Of the original 116 items, 11 items had limited applicability; 13 items had unclear references; 32 items had lexical problems and 11 items had logical problems. In total, 29 items were deleted, 31 retained and 56 revised. Thematic analysis revealed participants limited their responses to consider only conventional practices such as grocery shopping, cooking and planned meals rather than the totality of their eating. An additional eighty-four items were developed to address eating out, incidental eating occasions and inconsistencies between participants assumed correct knowledge and that of public health guidelines. This resulted in a refined 171-item pool.Conclusions:This study progresses the development towards a comprehensive, validated food literacy questionnaire.
Solar Wind—Magnetosphere Coupling During Radial Interplanetary Magnetic Field Conditions: Simultaneous Multi‐Point Observations
Authors: Toledo‐Redondo S. , Hwang K.‐J. , Escoubet C. P. , Lavraud B. , Fornieles J. , Aunai N. , Fear R. C. , Dargent J. , Fu H. S. , Fuselier S. A. , Genestreti K. J. , Khotyaintsev Yu V. , Li W. Y. , Norgren C. , Phan T. D. 
Published: November 2021

AbstractIn‐situ spacecraft missions are powerful assets to study processes that occur in space plasmas. One of their main limitations, however, is extrapolating such local measurements to the global scales of the system. To overcome this problem at least partially, multi‐point measurements can be used. There are several multi‐spacecraft missions currently operating in the Earth's magnetosphere, and the simultaneous use of the data collected by them provides new insights into the large‐scale properties and evolution of magnetospheric plasma processes. In this work, we focus on studying the Earth's magnetopause (MP) using a conjunction between the Magnetospheric Multiscale and Cluster fleets, when both missions skimmed the MP for several hours at distant locations during radial interplanetary magnetic field (IMF) conditions. The observed MP positions as a function of the evolving solar wind conditions are compared to model predictions of the MP. We observe an inflation of the magnetosphere (∼0.7 RE), consistent with magnetosheath pressure decrease during radial IMF conditions, which is less pronounced on the flank (0.2 RE). There is observational evidence of magnetic reconnection in the subsolar region for the whole encounter, and in the dusk flank for the last portion of the encounter, suggesting that reconnection was extending more than 15 RE. However, reconnection jets were not always observed, suggesting that reconnection was patchy, intermittent or both. Shear flows reduce the reconnection rate up to ∼30% in the dusk flank according to predictions, and the plasma β enhancement in the magnetosheath during radial IMF favors reconnection suppression by the diamagnetic drift.
Improved Estimates of Pentad Precipitation Through the Merging of Independent Precipitation Data Sets
Authors: Koster Randal D. , Liu Qing , Reichle Rolf H. , Huffman George J. 
Published: November 2021

AbstractThree independent, quasi‐global, gridded data sets of precipitation (a rain gauge‐based data set, the satellite‐only component of the NASA Integrated Multi‐satellitE Retrievals for Global Precipitation Measurement mission (IMERG) Final Run precipitation product, and precipitation estimates derived from NASA Soil Moisture Active Passive (SMAP) soil moisture retrievals), are objectively combined into a single pentad precipitation data set at 36‐km resolution using a unique approach based on extended triple collocation. The quality of each of the four data sets is then evaluated against independent observations. When a global land surface model at 36‐km resolution is integrated four times, once utilizing the merged precipitation forcing and once with each of the three contributing data sets, the near‐surface soil moisture variations produced with the merged forcing validate best against independent satellite‐based soil moisture fields. In addition, the merged data set is found to be more consistent, relative to each contributor, with estimates of air temperature variations across the globe. The merged data set thus appears to draw successfully on the complementary strengths of each contributor: the particularly high quality of the rain gauge‐based data set in areas of high gauge density, the more uniform accuracy across the globe of the IMERG data, and the moderate accuracy, particularly in semi‐arid regions, of the soil moisture retrieval‐based data.
Targeting lysosomes in human disease: from basic research to clinical applications
Authors: Cao Mengdie , Luo Xiangyuan , Wu Kongming , He Xingxing 
Published: November 2021

AbstractIn recent years, accumulating evidence has elucidated the role of lysosomes in dynamically regulating cellular and organismal homeostasis. Lysosomal changes and dysfunction have been correlated with the development of numerous diseases. In this review, we interpreted the key biological functions of lysosomes in four areas: cellular metabolism, cell proliferation and differentiation, immunity, and cell death. More importantly, we actively sought to determine the characteristic changes and dysfunction of lysosomes in cells affected by these diseases, the causes of these changes and dysfunction, and their significance to the development and treatment of human disease. Furthermore, we outlined currently available targeting strategies: (1) targeting lysosomal acidification; (2) targeting lysosomal cathepsins; (3) targeting lysosomal membrane permeability and integrity; (4) targeting lysosomal calcium signaling; (5) targeting mTOR signaling; and (6) emerging potential targeting strategies. Moreover, we systematically summarized the corresponding drugs and their application in clinical trials. By integrating basic research with clinical findings, we discussed the current opportunities and challenges of targeting lysosomes in human disease.
Stem cell-based therapy for COVID-19 and ARDS: a systematic review
Authors: Zanirati Gabriele , Provenzi Laura , Libermann Lucas Lobraico , Bizotto Sabrina Comin , Ghilardi Isadora Machado , Marinowic Daniel Rodrigo , Shetty Ashok K. , Da Costa Jaderson Costa 
Published: November 2021

AbstractDespite global efforts to establish effective interventions for coronavirus disease 2019 (COVID-19) and its major complications, such as acute respiratory distress syndrome (ARDS), the treatment remains mainly supportive. Hence, identifying an effective and safe therapy for severe COVID-19 is critical for saving lives. A significant number of cell-based therapies have been through clinical investigation. In this study, we performed a systematic review of clinical studies investigating different types of stem cells as treatments for COVID-19 and ARDS to evaluate the safety and potential efficacy of cell therapy. The literature search was performed using PubMed, Embase, and Scopus. Among the 29 studies, there were eight case reports, five Phase I clinical trials, four pilot studies, two Phase II clinical trials, one cohort, and one case series. Among the clinical studies, 21 studies used cell therapy to treat COVID-19, while eight studies investigated cell therapy as a treatment for ARDS. Most of these (75%) used mesenchymal stem cells (MSCs) to treat COVID-19 and ARDS. Findings from the analyzed articles indicate a positive impact of stem cell therapy on crucial immunological and inflammatory processes that lead to lung injury in COVID-19 and ARDS patients. Additionally, among the studies, there were no reported deaths causally linked to cell therapy. In addition to standard care treatments concerning COVID-19 management, there has been supportive evidence towards adjuvant therapies to reduce mortality rates and improve recovery of care treatment. Therefore, MSCs treatment could be considered a potential candidate for adjuvant therapy in moderate-to-severe COVID-19 cases and compassionate use.
Fenebrutinib in H1 antihistamine-refractory chronic spontaneous urticaria: a randomized phase 2 trial
Authors: Metz Martin , Sussman Gordon , Gagnon Rémi , Staubach Petra , Tanus Tonny , Yang William H. , Lim Jeremy J. , Clarke Holly J. , Galanter Joshua , Chinn Leslie W. , Chu Tom , Teterina Anastasia , Burgess Tracy , Haddon D. James , Lu Timothy T. , Maurer Marcus 
Published: November 2021

AbstractBruton’s tyrosine kinase (BTK) is crucial for FcεRI-mediated mast cell activation and essential for autoantibody production by B cells in chronic spontaneous urticaria (CSU). Fenebrutinib, an orally administered, potent, highly selective, reversible BTK inhibitor, may be effective in CSU. This double-blind, placebo-controlled, phase 2 trial (EudraCT ID2016-004624-35) randomized 93 adults with antihistamine-refractory CSU to 50 mg daily, 150 mg daily and 200 mg twice daily of fenebrutinib or placebo for 8 weeks. The primary end point was change from baseline in urticaria activity score over 7 d (UAS7) at week 8. Secondary end points were the change from baseline in UAS7 at week 4 and the proportion of patients well-controlled (UAS7 ≤ 6) at week 8. Fenebrutinib efficacy in patients with type IIb autoimmunity and effects on IgG-anti-FcεRI were exploratory end points. Safety was also evaluated. The primary end point was met, with dose-dependent improvements in UAS7 at week 8 occurring at 200 mg twice daily and 150 mg daily, but not at 50 mg daily of fenebrutinib versus placebo. Asymptomatic, reversible grade 2 and 3 liver transaminase elevations occurred in the fenebrutinib 150 mg daily and 200 mg twice daily groups (2 patients each). Fenebrutinib diminished disease activity in patients with antihistamine-refractory CSU, including more patients with refractory type IIb autoimmunity. These results support the potential use of BTK inhibition in antihistamine-refractory CSU.
Combinatorial characterization of a certain class of words and a conjectured connection with general subclasses of phylogenetic tree-child networks
Authors: Pons Miquel , Batle Josep 
Published: November 2021

AbstractThe combinatorial study of phylogenetic networks has attracted much attention in recent times. In particular, one class of them, the so-called tree-child networks, are becoming the most prominent ones. However, their combinatorial properties are largely unknown. In this paper we address the problem of exactly counting them. We conjecture a relationship with the cardinality of a certain class of words. By solving the counting problem for the words, and on the basis of the conjecture, several simple recurrence formulas for general cases arise. Moreover, a precise asymptotic analysis is provided. Our results coincide with all current formulas in the literature for particular subclasses of tree-child networks, as well as with numerical results obtained for small networks. We expect that the study of the relationship between the newly defined words and the networks will lead to further combinatoric characterizations of this class of phylogenetic networks.
Confirming the TMEM232 gene associated with atopic dermatitis through targeted capture sequencing
Authors: Zheng Jie , Wu Yuan-yuan , Fang Wen-liang , Cai Xin-ying , Zhang Zeng-yun-ou , Yu Chong-xian , Zheng Xiao-dong , Xiao Feng-li 
Published: November 2021

AbstractAtopic dermatitis (AD) is a common and complex skin disorder, and the 5q22.1 region had been reported to be associated with AD. To confirm the susceptibility gene for AD in the 5q22.1 region by haplotype and targeted capture sequencing. The haplotypes were reconstructed with the genotyping data of four SNPs and six deletions from 3624 Chinese Hans AD patients and 5076 controls. The targeted capture sequencing spanning 5q22.1 region was performed in the selected samples. The gene level enrichment analysis was done using loss of function variants. A total of 62 haplotypes were found, and the H15 haplotype had the strongest association with AD (P = 3.92 × 10−10, OR 0.17, 95% CI 0.09–0.32). However, no co-segregation mutation sites were found in the sequencing analysis within the 16 selected samples, while the enrichment analysis indicated that TMEM232 was significantly associated with AD (P = 7.33 × 10–5, OR 0.33, 95% CI 0.19–0.58). This study confirms previous findings that the TMEM232 gene is associated with AD by haplotype analysis and targeted capture sequencing.
Characterisation of insomnia as an environmental risk factor for asthma via Mendelian randomization and gene environment interaction
Authors: Kim Dong Jun , Ha Tae-Woong , Jung Hae Un , Baek Eun Ju , Lee Won Jun , Kim Han Kyul , Kang Ji-One , Won Sungho , Lim Ji Eun , Oh Bermseok 
Published: November 2021

AbstractAsthma is a complex disease that is reportedly associated with insomnia. However, the causal directionality of this association is still unclear. We used asthma and insomnia-associated single nucleotide polymorphisms (SNPs) and genome-wide association study (GWAS) summary statistics to test the causal directionality between insomnia and asthma via Mendelian randomization (MR) analysis. We also performed a cross-trait meta-analysis using UK Biobank GWAS summary statistics and a gene–environment interaction study using data from UK Biobank. The interaction of genetic risk score for asthma (GRSasthma) with insomnia on asthma was tested by logistic regression. Insomnia was a risk factor for the incidence of asthma, as revealed by three different methods of MR analysis. However, asthma did not act as a risk factor for insomnia. The cross-trait meta-analysis identified 28 genetic loci shared between asthma and insomnia. In the gene–environment interaction study, GRSasthma interacted with insomnia to significantly affect the risk of asthma. The results of this study highlight the importance of insomnia as a risk factor of asthma, and warrant further analysis of the mechanism through which insomnia affects the risk of asthma.
A deep learning approach to identify and segment alpha-smooth muscle actin stress fiber positive cells
Authors: Hillsley Alexander , Santos Javier E. , Rosales Adrianne M. 
Published: November 2021

AbstractCardiac fibrosis is a pathological process characterized by excessive tissue deposition, matrix remodeling, and tissue stiffening, which eventually leads to organ failure. On a cellular level, the development of fibrosis is associated with the activation of cardiac fibroblasts into myofibroblasts, a highly contractile and secretory phenotype. Myofibroblasts are commonly identified in vitro by the de novo assembly of alpha-smooth muscle actin stress fibers; however, there are few methods to automate stress fiber identification, which can lead to subjectivity and tedium in the process. To address this limitation, we present a computer vision model to classify and segment cells containing alpha-smooth muscle actin stress fibers into 2 classes (α-SMA SF+and α-SMA SF-), with a high degree of accuracy (cell accuracy: 77%, F1 score 0.79). The model combines standard image processing methods with deep learning techniques to achieve semantic segmentation of the different cell phenotypes. We apply this model to cardiac fibroblasts cultured on hyaluronic acid-based hydrogels of various moduli to induce alpha-smooth muscle actin stress fiber formation. The model successfully predicts the same trends in stress fiber identification as obtained with a manual analysis. Taken together, this work demonstrates a process to automate stress fiber identification in in vitro fibrotic models, thereby increasing reproducibility in fibroblast phenotypic characterization.
Renal and hepatic function of patients with severe tricuspid regurgitation undergoing inferior caval valve implantation
Authors: Hewing Bernd , Mattig Isabel , Knebel Fabian , Stangl Verena , Laule Michael , Stangl Karl , Dreger Henryk 
Published: November 2021

AbstractDue to progressive abdominal-venous congestion severe tricuspid regurgitation (TR) is a common cause of cardiorenal and cardiohepatic syndrome. We initiated the TRICAVAL study to compare interventional valve implantation into the inferior vena cava (CAVI) versus optimal medical therapy (OMT) in severe TR. In the present subanalysis, we aimed to evaluate the effects of CAVI on clinical signs of congestion, renal and hepatic function. TRICAVAL was an investigator-initiated, randomized trial. Twenty-eight patients with severe TR were randomized to OMT or CAVI using an Edwards Sapien XT valve. Probands who completed the 3-month follow-up (CAVI [n = 8], OMT [n = 10]) were evaluated by medical history, clinical examination, and laboratory testing at baseline, 3 and 12 months. After 3 months, the CAVI group exhibited a significant reduction of body weight (from 80.7 [69.0–87.7] kg to 75.5 [63.8–84.6] kg, p < 0.05) and abdominal circumference (from 101.5 ± 13.8 cm to 96.3 ± 15.4 cm, p ≤ 0.01) and a trend to lower doses of diuretics compared to OMT. Renal and hepatic function parameters did not change significantly. Within a short-term follow-up, CAVI led to an improvement of clinical signs of venous congestion and a non-significant reduction of diuretic doses compared to OMT.