bluuebunny/crossref_metadata_2025_split huggingface dataset:
Frequency of auto‐antibodies of type 1 diabetes in adult patients with celiac disease
Authors: Ghozzi Mariam , Souguir Dorra , Melayah Sarra , Abidi Skander , Faleh Mariem , Ghedira Ibtissem 
Published: August 2021

AbstractBoth celiac disease (CD) and type 1 diabetes (T1D) are autoimmune diseases resulting from a complex interplay between genetic susceptibility and environmental factors.AimIn this retrospective study, we determined the frequency of auto‐antibodies of T1D in adult patients with active CD.Materials and methodsEighty adult patients with active CD were included in our study. Ninety healthy blood donors (HBD) served as control group. Anti‐glutamic acid decarboxylase IgG antibodies (GAD‐Ab), anti‐tyrosine phosphatase IgG antibodies (IA2‐Ab), and anti‐zinc transporter IgG antibodies (Zn‐T8‐Ab) were determined by enzyme‐linked immunosorbent assay (ELISA) for patients and control group. For statistical analysis, we used Chi‐square or Fisher's exact test.ResultsOut of 80 patients, 10 (12.50%) had auto‐antibodies of T1D vs. only one in control group (1.11%) (p = 0.003). Simultaneous presence of GAD‐Ab, IA2‐Ab, and Zn‐T8‐Ab was found in one patient (1.25%). Nine patients had only GAD‐Ab. IA2‐Ab and Zn‐T8‐Ab were absent in all HBD. The frequency of GAD‐Ab was significantly higher in CD patients than in HBD (12.5% vs 1.11%, p = 0.003).ConclusionThe present study has shown that CD is associated with a high frequency of auto‐antibodies of T1D. Screening for T1D in this population, at risk for other autoimmune diseases, may be useful.
Effect of hydrogen sulfide on glycolysis‐based energy production in mouse erythrocytes
Authors: Wondimu Eden T. , Zhang Quanxi , Jin Zhuping , Fu Ming , Torregrossa Roberta , Whiteman Matthew , Yang Guangdong , Wu Lingyun , Wang Rui 
Published: August 2021

AbstractHydrogen sulfide (H2S) is a gasotransmitter that regulates both physiological and pathophysiological processes in mammalian cells. Recent studies have demonstrated that H2S promotes aerobic energy production in the mitochondria in response to hypoxia, but its effect on anaerobic energy production has yet to be established. Glycolysis is the anaerobic process by which ATP is produced through the metabolism of glucose. Mammalian red blood cells (RBCs) extrude mitochondria and nucleus during erythropoiesis. These cells would serve as a unique model to observe the effect of H2S on glycolysis‐mediated energy production. The purpose of this study was to determine the effect of H2S on glycolysis‐mediated energy production in mitochondria‐free mouse RBCs. Western blot analysis showed that the only H2S‐generating enzyme expressed in mouse RBCs is 3‐mercaptopyruvate sulfurtransferase (MST). Supplement of the substrate for MST stimulated, but the inhibition of the same suppressed, the endogenous production of H2S. Both exogenously administered H2S salt and MST‐derived endogenous H2S stimulated glycolysis‐mediated ATP production. The effect of NaHS on ATP levels was not affected by oxygenation status. On the contrary, hypoxia increased intracellular H2S levels and MST activity in mouse RBCs. The mitochondria‐targeted H2S donor, AP39, did not affect ATP levels of mouse RBCs. NaHS at low concentrations (3–100 μM) increased ATP levels and decreased cell viability after 3 days of incubation in vitro. Higher NaHS concentrations (300–1000 μM) lowered ATP levels, but prolonged cell viability. H2S may offer a cytoprotective effect in mammalian RBCs to maintain oxygen‐independent energy production.
BAIAP2L2 is required for the maintenance of mechanotransducing stereocilia of cochlear hair cells
Authors: Yan Keji , Zong Wen , Du Haibo , Zhai Xiaoyan , Ren Rui , Liu Shuang , Xiong Wei , Wang Yanfei , Xu Zhigang 
Published: August 2021

AbstractStereocilia are actin‐based cell protrusions of inner ear hair cells that play an essential role in mechano‐electrical transduction (MET). Stereocilia are organized into several rows of increasing heights with the MET protein complex localized at the tips of shorter row stereocilia. At the tips of shorter row mechanotransducing stereocilia also resides a so‐called “row 2 protein complex” whose dysfunction causes degeneration of the mechanotransducing stereocilia. In the present work, we show that BAIAP2L2 is localized at the tips of shorter row stereocilia in neonatal and adult mouse cochlear hair cells. Baiap2l2 inactivation causes degeneration of the mechanotransducing stereocilia, which eventually leads to profound hearing loss in mice of either sex. Consistently, electrophysiology and FM 1‐43FX dye uptake results confirm that MET currents are compromised in Baiap2l2 knockout mice. Moreover, BAIAP2L2 binds to known row 2 complex components EPS8L2, TWF2, and CAPZB2, and the stereociliary tip localization of CAPZB2 is dependent on functional BAIAP2L2. Interestingly, BAIAP2L2 also binds to CIB2, a known MET complex component, and the stereociliary tip localization of BAIAP2L2 is abolished in Cib2 knockout mice. In conclusion, our present data suggest that BAIAP2L2 is a row 2 complex component, and is required for the maintenance of mechanotransducing stereocilia. Meanwhile, specific MET components such as CIB2 might play a direct role in stereocilia maintenance through binding to BAIAP2L2.
Comparison of seven heterophile antibody assays for laboratory diagnosis of infectious mononucleosis in pediatric patients
Authors: Wei Sharon , Huang Wei , Farah Mohamed , Khanolkar Aaruni , Zheng Xiaotian 
Published: August 2021

AbstractHeterophile antibody assays have been used to aid the diagnosis of infectious mononucleosis caused by the Epstein‐Barr virus. Seven commercially available assays currently widely utilized in clinical laboratories were compared in this study. Variable performance characteristics and assay times are observed, and these pieces of data may assist clinical laboratories in assay selection and result interpretation.
Is cyclooxygenase‐1 involved in neuroinflammation?
Authors: Ghazanfari Nafiseh , van Waarde Aren , Dierckx Rudi A. J. O. , Doorduin Janine , de Vries Erik F. J. 
Published: August 2021

AbstractPurpose: Reactive microglia are an important hallmark of neuroinflammation. Reactive microglia release various inflammatory mediators, such as cytokines, chemokines, and prostaglandins, which are produced by enzymes like cyclooxygenases (COX). The inducible COX‐2 subtype has been associated with inflammation, whereas the constitutively expressed COX‐1 subtype is generally considered as a housekeeping enzyme. However, recent evidence suggests that COX‐1 can also be upregulated and may play a prominent role in the brain during neuroinflammation. In this review, we summarize the evidence that supports this involvement of COX‐1. Methods: Five databases were used to retrieve relevant studies that addressed COX‐1 in the context of neuroinflammation. The search resulted in 32 articles, describing in vitro, in vivo, post mortem, and in vivo imaging studies that specifically investigated the COX‐1 isoform under such conditions. Results: Reviewed literature generally indicated that the overexpression of COX‐1 was induced by an inflammatory stimulus, which resulted in an increased production of prostaglandin E2. The pharmacological inhibition of COX‐1 was shown to suppress the induction of inflammatory mediators like prostaglandin E2. Positron emission tomography (PET) imaging studies in animal models confirmed the overexpression of COX‐1 during neuroinflammation. The same imaging method, however, could not detect any upregulation of COX‐1 in patients with Alzheimer's disease. Conclusion: Taken together, studies in cultured cells and living rodents suggest that COX‐1 is involved in neuroinflammation. Most postmortem studies on human brains indicate that the concentration of COX‐1‐expressing microglial cells is increased near sites of inflammation. However, evidence for the involvement of COX‐1 in neuroinflammation in the living human brain is still largely lacking.
A case‐control study on the association between periodontitis and coronavirus disease (COVID‐19)
Authors: Anand Pradeep S. , Jadhav Pranavi , Kamath Kavitha P. , Kumar Salavadi Revanth , Vijayalaxmi Sandapola , Anil Sukumaran 
Published: August 2021

AbstractBackgroundCoronavirus disease (COVID‐19) and periodontitis share common characteristics, such as an exaggerated inflammatory response. As periodontal diseases were shown to be associated with respiratory diseases, such as pneumonia, it is quite possible that a relationship may exist between periodontitis and COVID‐19. Hence, the aim of the present study was to determine whether periodontitis and poor oral hygiene are associated with COVID‐19.MethodsA case‐control study was conducted. Patients who had positive real‐time reverse transcription polymerase chain reaction results for severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) infection were included in the case group (n = 79), and patients with negative results were included in the control group (n = 71). The periodontal examination involved recording the plaque scores, calculus scores, tooth mobility, gingival bleeding, probing depth, recession, and clinical attachment level (CAL).ResultsLogistic regression analysis showed significant associations of mean plaque scores ≥ 1 (odds ratio (OR), 7.01; 95% confidence interval (CI), 1.83 to 26.94), gingivitis (OR, 17.65; 95% CI, 5.95 to 52.37), mean CAL ≥ 2 mm (OR, 8.46; 95% CI, 3.47 to 20.63), and severe periodontitis (OR, 11.75; 95% CI, 3.89 to 35.49) with COVID‐19; these findings were more prevalent in the case group.ConclusionBased on the above mentioned observations, it can be concluded that there is an association between periodontitis severity and COVID‐19. Gingival bleeding and dental plaque accumulation are also more frequent among COVID‐19 patients. Hence, it is essential to maintain periodontal health and good oral hygiene as an important measure for COVID‐19 prevention and management.
Summer pruning of sweet cherry: a way to control sugar content in different organs
Authors: Vosnjak Matej , Mrzlic Davor , Usenik Valentina 
Published: August 2021

AbstractBACKGROUNDSweet cherry trees (Prunus aviumL.) of the cultivar Grace Star were pruned either in dormancy or in summer. The response was studied by analyzing the sugar content in different organs (flower bud, leaf, and fruit) at three sections of the canopy (inner, outer, and upper) using high‐performance liquid chromatography. The effect of summer pruning was evaluated by measuring photosynthetic photon flux density (PPFD) and leaf chlorophyll content (SPAD).RESULTSIn this study, the timing of pruning had a significant effect on sugar content in flower buds, leaves, and fruit. Trees pruned in summer had higher glucose, fructose, sorbitol, and sucrose content in flower buds, higher glucose and fructose contents in leaves, and lower fructose, sorbitol, and total sugar content in fruit than in trees pruned at dormancy. Higher average PPFD and lower SPAD values were measured in the inner canopy of trees pruned in summer. All measured parameters were influenced by position in the canopy. The lowest fructose and sorbitol contents in the flower bud, the lowest content of glucose, fructose, sorbitol, total sugars and the highest SPAD values in the leaf, while less dark and lighter fruit were measured in the inner part of the canopy.CONCLUSIONSummer pruning affects sugar distribution in the tree by altering irradiation conditions within the canopy. Our results suggest that summer pruning is an effective technological measure to improve sugar content in the buds. A strong, well nourished flower bud is a good indication of high fruit production next season. © 2021 Society of Chemical Industry.
A rapid ultra high performance liquid chromatography–tandem mass spectrometry method for the quantification of daidzein, its valine carbamate prodrug, and glucuronide in rat plasma samples: Comparison of the pharmacokinetic behavior of daidzine valine carbamate prodrugs
Authors: Li Yingchao , Lu Farong , Zhang Yawei , Liu Xiaoyu , Lin Longyi , Jiang Qikun , Zhang Tianhong 
Published: August 2021

AbstractTwo valine carbamate prodrugs of daidzein were designed to improve its bioavailability. To compare the pharmacokinetic behavior of these prodrugs with different protected phenolic hydroxyl groups of daidzein, a rapid and sensitive method for simultaneous quantification of daidzein, its valine carbamate prodrug, and daidzein‐7‐O‐glucuronide in rat plasma was developed and validated in this study. The samples were processed using a fast one‐step protein precipitation method with methanol added to 50 μL of plasma and were analyzed by ultra‐high performance liquid chromatography with tandem mass spectrometry. To improve the selectivity, peak shape, and peak elution, several key factors, especially stationary phase and the composition of the mobile phase, were tested, and the analysis was performed using the Kinetex®C18 column (100 × 2.1 mm, 2.6 μm) within only 2.6 min under optimal conditions. The established method exhibited good linearity over the concentration range of 2.0–1000 ng/mL for daidzein, and 8.0–4000 ng/mL for the prodrug and daidzein‐7‐O‐glucuronide. The accuracy of the quality control samples was between 95.5 and 110.2% with satisfactory intra‐ and interday precision (relative standard deviation values < 10.85%), respectively. This sensitive, rapid, low‐cost, and high‐throughput method was successfully applied to compare the pharmacokinetic behavior of different daidzein carbamate prodrugs.
Focal nodular hyperplasia after systemic chemotherapy: Pathological features of a series of 15 cases
Authors: Brunet Anaïs , Beaufrère Aurélie , Cauchy François , Paradis Valérie 
Published: August 2021

AbstractIntroductionChemotherapy, particularly oxaliplatin, has been associated with the development of focal nodular hyperplasia (FNH). Imaging diagnosis of FNH is well standardized, but it can be misdiagnosed as liver metastasis. The aim of this study was to describe the pathological features of FNH occurring after systemic chemotherapy.Materials and methodsFrom our pathological files for 1990‐2021, we retrieved 15 cases of resected newly developed FNH in adults with liver metastasis treated with systemic chemotherapy. Pathological features of FNH nodules and non‐tumoral liver samples were reviewed.ResultsIn 11/15 (73%) cases, FNH developed after an oxaliplatin‐based regimen. The median interval from the beginning of chemotherapy to the FNH diagnosis was 15 months. FNH was unique in 11 (73%) cases, and the median size of nodules was 1.1 cm [range 0.5‐2.5]. Histologically, 9 (60%), 11 (73%) and 11 (73%) cases exhibited fibrous central scar, dystrophic vessels and ductular proliferation, respectively, with all three criteria present in five (33%) cases. Eight (53%) cases showed intralesional steatosis and nine (60%) cases showed a glutamine synthetase immunostaining map‐like pattern. In non‐tumoral liver, eight (53%) cases exhibited sinusoidal obstruction syndrome and four (27%) nodular regenerative hyperplasia.ConclusionThe occurrence of FNH after systemic chemotherapy is an emerging condition challenging the imaging diagnosis because typical morphological features are frequently missing. The presence of sinusoidal changes, including regenerative hyperplasia, in non‐tumoral liver supports the potential role of chemotherapy in the pathogenesis of FNH.
Beyond craving: Appetitive desire as a motivational antecedent of goal‐directed action intentions
Authors: Aguirre‐Rodriguez Alexandra , Bagozzi Richard P. , Torres Patricia L. 
Published: August 2021

AbstractSelf‐regulation is generally considered a process of constraining appetitive desire motivation toward goal‐inconsistent objects and actions through rationality‐based (volitive) motivation. The predominant temptation‐focused view of appetitive desire focuses on cravings and self‐control dilemmas, overlooking the beneficial role of appetitive desire in motivating many types of goal‐directed action intentions. We extend this line of research based in the philosophy of action theoretical perspective by proposing that appetitive desire is broader than cravings and it positively influences goal‐consistent action intentions by strengthening volitive desire motivation. Appetitive desire toward goal means containing pleasurable features provides reasons for enacting goal‐consistent actions, which energizes intention formation through volitive desire. Thus, appetitive desire often plays an instrumental rather than detrimental motivational role in reasoned action toward goal pursuit. Three studies, one measurement‐based and two experimental, involving different goal‐directed action contexts, demonstrate that goal means associated with high (vs. low) appetitive desire result in greater volitive desire, which strengthens intentions to enact the goal means. Our results contribute to an expanded understanding of appetitive desire, beyond craving and temptation, as a positive antecedent in the causal sequences of appetitive desire→volitive desire→intentions in the context of consumer goal pursuit.
Modulation of Aggregation‐Caused Quenching to Aggregation‐Induced Emission: Finding a Biocompatible Polymeric Theranostics Platform for Cancer Therapy
Authors: Wang Cheng , Wang Ziyu , Chen Shaoqing , Cui Pengfei , Qiu Lin , Zhou Shuwen , Jiang Hua , Jiang Pengju , Wang Jianhao 
Published: August 2021

AbstractDual intramolecular FRET polymers are synthesized via Suzuki coupling and their luminescence characteristics from aggregation‐caused quenching (ACQ) to aggregation‐induced emission (AIE) is modulated conveniently by adjusting the charged ratios. The finally obtained AIE polymer is further employed to construct doxorubicin loaded nanoparticles as a promising theranostics platform for cancer therapy.
Sustainable Hyperbranched Functional Materials via Green Polymerization of Readily Accessible Levoglucosenone‐Derived Monomers
Authors: Fadlallah Sami , Flourat Amandine L. , Mouterde Louis M. M. , Annatelli Mattia , Peru Aurélien A. M. , Gallos Antoine , Aricò Fabio , Allais Florent 
Published: August 2021

AbstractThe homopolymerization in basic conditions of the recently reported bis(γ‐lactone), 2H‐HBO‐HBO, is herein described for the first time. The solvent‐free polymerization of this pentafunctional levoglucosenone (LGO) derivative affords fully renewable poly(vinyl‐ether lactone) copolymers with a highly hyperbranched structure. This investigation stems from the polycondensation trials between 2H‐HBO‐HBO and di(methyl carbonate) isosorbide (DCI) that fails to give the anticipated polycarbonates. Such unexpected behavior is ascribed to the higher reactivity of the 2H‐HBO‐HBO hydroxy groups toward its α,β‐conjugated endocyclic C═C, rather than the DCI methylcarbonate moieties. The different mechanistic scenarios involved in 2H‐HBO‐HBO homopolymerization are addressed and a possible structure of poly(2H‐HBO‐HBO) is suggested. Furthermore, the readily accessible (S)‐γ‐hydroxymethyl‐α,β‐butenolide (HBO) is also polymerized for the first time at a relatively large scale, without any prior modification, resulting in a new hyperbranched polymer with an environmental factor (E factor) ≈0. These new HBO‐based polymers have a great potential for industrial‐scale production due to their interesting properties and easy preparation via a low‐cost, green, and efficient process.
A PROXYL‐Type Norbornene Polymer for High‐Voltage Cathodes in Lithium Batteries
Authors: Hatakeyama‐Sato Kan , Matsumoto Satoshi , Takami Hirofumi , Nagatsuka Tomomi , Oyaizu Kenichi 
Published: August 2021

AbstractA newly designed radical polymer with a polynorbornene backbone and unsaturated derivative of tetramethylpyrrolidine 1‐oxyl (PROXYL) as pendant groups displays reversible redox at 3.75 V (vs Li/Li+). The robust polymer design enables the high voltage while maintaining a promising cyclability (over 1000 cycles). The polymer is also beneficial as an additive to the regular lithium iron phosphate electrodes, where the quickly responding organic material facilitates the charging reactions catalytically.
A potential signaling axis between RON kinase receptor and hypoxia‐inducible factor‐1 alpha in pancreatic cancer
Authors: Kato Akihisa , Ng Serina , Thangasamy Amalraj , Han Haiyong , Zhou Wendi , Raeppel Stephane , Fallon Michael , Guha Sushovan , Ammanamanchi Sudhakar 
Published: August 2021

AbstractThe Cancer Genome Atlas (TCGA) of a pancreatic cancer cohort identified high MST1R (RON tyrosine kinase receptor) expression correlated with poor prognosis in human pancreatic cancer. RON expression is null/minimal in normal pancreas but elevates from pan‐in lesions through invasive carcinomas. We report using multiple approaches RON directly regulates HIF‐1α, a critical driver of genes involved in cancer cell invasion and metastasis. RON and HIF‐1α are highly co‐expressed in the 101 human PDAC tumors analyzed and RON expression correlated with HIF‐1α expression in a subset of PDAC cell lines. knockdown of RON expression in RON positive cells blocked HIF‐1α expression, whereas ectopic RON expression in RON null cells induced HIF‐1α expression suggesting the direct regulation of HIF‐1α by RON kinase receptor. RON regulates HIF‐1α through an unreported transcriptional mechanism involving PI3 kinase‐mediated AKT phosphorylation and Sp1‐dependent HIF‐1α promoter activity leading to increased HIF‐1α mRNA expression. RON/HIF‐1α modulation altered the invasive behavior of PDAC cells. A small‐molecule RON kinase inhibitor decreased RON ligand, MSP‐induced HIF‐1α expression, and invasion of PDAC cells. Immunohistochemical analysis on RON knockdown orthotopic PDAC tumor xenograft confirmed that RON inhibition significantly blocked HIF‐1α expression. RON/HIF‐1α co‐expression also exists in triple‐negative breast cancer cells, a tumor type that also lacks molecular therapeutic targets. This is the first report describing RON/HIF‐1α axis in any tumor type and is a potential novel therapeutic target.
Proxy evaluation of dignity expectations and satisfaction of older patients with dementia by family members and nurses
Authors: Otake Eriko , Ota Katsumasa , Ikegami Chikako , Niimi Yukari , Yamada Satoko , Maeda Jukai , Matsuda Masami 
Published: August 2021

AbstractAimDevelop a proxy evaluation questionnaire for patients' family members and nurses to evaluate dignity expectations and satisfaction of patients with dementia.DesignCross‐sectional study.MethodA proxy questionnaire draft was prepared with 30 items on expectations for dignity and 23 items on satisfaction with dignity. And administered to three paired groups: 81 older patients with intact cognitive function, 75 family members, and 77 nurses.Results18 of 30 items and 21 of 23 were correlated between patients and their family members' responses on dignity expectations and satisfaction, respectively. When limited to nurses with clinical experience of ≥20 years, there were significant correlations between patients' and nurses' responses (p < .05). Exploratory factor analysis of patient's responses to significantly correlated items extracted 3 factors with 13 items of expectations for dignity but no factors of satisfaction with dignity. Using a questionnaire provides insight for proxy evaluation of expectations for dignity.