bluuebunny/crossref_metadata_2025_split huggingface dataset:
Novel Dihydroartemisinin Derivative Mito-DHA5 Induces Apoptosis Associated with Mitochondrial Pathway in Bladder Cancer Cells
Authors: Xiao Linfan , Xu Cangcang , Lin Peiyu , Mu Lingli , Yang Xiaoping 
Published: September 2021

Abstract
Background: Bladder cancer is the second most common genitourinary malignancy and the eleventh most common cancer worldwide. Dihydroartemisinin (DHA), a first-line antimalarial drug, has been found to have potent antitumor activity. In our previous study, a novel dihydroartemisinin derivative Mito-DHA5 synthesized in our laboratory has a stronger anti-tumor activity than DHA. In this study, we investigated the apoptotic effect of Mito-DHA5 on bladder cancer T24 cells and molecular mechanisms underlying. Methods: Antitumor activity in vitro was evaluated by MTT and cloning formation assays. Mitochondrial membrane potential (MMP) was detected by JC-1 probe and ROS levels were measured by specific kit. The expression of caspase-3, Bcl-2 and Bax in T24 cells was evaluated by Western blotting. Results: The results showed that Mito-DHA5 reduced cell viability with an IC50 value of 3.2 μM in a dose-dependent manner, induced T24 cell apoptosis at both early and late stages, increased the production of ROS and decreased MMP. Mito-DHA5 could down-regulate the expression of Bcl-2 and Caspase-3, and up-regulate the expression of Bax and cleaved Caspase-3. Conclusion: These data suggested that Mito-DHA5 had a potent inhibitory effect on T24 bladder cancer cell growth and induced these cells apoptosis associated with mitochondrial pathway.
Microvascular Changes In The Recurrent Cystoid Macular Edema Secondary to Posterior Noninfectious Uveitis on Optical Coherence Tomography Angiography
Authors: Albano Valeria , Guerriero Silvana , Furino Claudio , Sborgia Giancarlo , Sborgia Alessandra , Dammacco Rosanna , Boscia Francesco , Alessio Giovanni 
Published: September 2021

Abstract
BACKGROUND: Posterior uveitis represents the second most frequent type of uveitis (15-30% of all uveitis). Non-infectious posterior uveitis complicated with secondary cystoid macular edema (CME) negatively affected the visual prognosis.The objective of the current study is to determine possible microvascular changes that can cause a relapsing uveitis-CME through optical coherence tomography angiography (OCTA).METHODS: This is a case-control study, an evaluation of patients with secondary CME noninfectious posterior uveitis-related undergoing dexamethasone (DEX) implant. The visits following the DEX-implant were carried out after 1 month, 2-months, 4-months, 6-months, and for up 1-year. A total of 76 eyes of 38 consecutive patients with noninfectious posterior uveitis were enrolled (consecutive sample). Complicated noninfectious posterior uveitis with secondary CME was diagnosed in 56 eyes of uveitis patients (24.3%) reviewed. RESULTS: Our investigation showed a reduction in superficial vessel plexus (SVP) measurements already within 2-month (84%), reaching 96.4% for up 1-year, however displaying an irregular profile in 69.6% of cases, persisting for up 1-year; the relapsing uveitis-CME eyes with irregular superficial foveal avascular zone (FAZ) profile were in 51%, while the SVP measurements reestablished in 100% of cases. Conversely, the deep vascular plexus (DVP) parameters restored occurred in a lower number of eyes within the 2-month (39.3%), remaining abnormal in 46.4% of cases for up 1-year; despite DVP restored in 53.6% of cases for up 1 year, a capillary rarefaction ring around the FAZ appeared in 80.4% of cases; the relapsing uveitis-CME eyes with abnormal DVP parameters were in 41% of cases, of which 92.1% showed a rarefaction ring had abnormal DVP.CONCLUSIONS: The use of OCTA allows to evaluate the retinal microvascular features, which could be the cause of the recurrence of CME in uveitis patients, despite the DEX-implant treatment. We suggested that the possibility of the recurrence of the uveitis-CME depends on the persistence of modifications of the superficial and deep layers, which we therefore consider appropriate to investigate. With this purpose it would be useful to introduce OCTA into the current imaging armamentarium in follow-up of patients with noninfectious uveitis-CME.
The Role of Air-Sea Coupling on November-April Intraseasonal Rainfall Variability Over the South Pacific
Authors: Pariyar Sunil Kumar , Keenlyside Noel , Tseng Wan-Ling , Hsu Huang Hsiung , Tsuang Ben-jei 
Published: September 2021

Abstract
We investigate the impact of resolving air-sea interaction on the simulation of the intraseasonal rainfall variability over the South Pacific using the ECHAM5 atmospheric general circulation model coupled with the Snow-Ice-Thermocline (SIT) ocean model. We compare the fully coupled simulation with two uncoupled ECHAM5 simulations, one forced with sea surface temperature (SST) climatology and one forced with daily SST from the coupled model. The intraseasonal rainfall variability over the South Pacific is reduced by 17% in the uncoupled model forced with SST climatology and increased by 8% in the uncoupled simulation forced with daily SST, suggesting the role of air-sea coupling and SST variability. The coupled model best simulates the key characteristics of two intraseasonal rainfall modes over the South Pacific with reasonable propagation and correct periodicity. The spatial structure of the two rainfall modes in all three simulations is very similar, suggesting these modes are primarily generated by the dynamics of the atmosphere. The southeastward propagation of rainfall anomalies associated with two leading rainfall modes in the South Pacific depends upon the eastward propagating MJO signals over the Indian Ocean and western Pacific. Air-sea interaction seems crucial for such propagation as both eastward and southeastward propagations are substantially reduced in the uncoupled model forced with SST climatology. The simulation of both eastward and southeastward propagations improved considerably in the uncoupled model forced with daily SST; however, the periodicity differs from the coupled model. Such discrepancy in the periodicity is attributed to the changes in the SST-rainfall relationship with weaker correlations and the nearly in-phase relationship.
Milk and Meat Consumption Patterns and the Potential Risk of Zoonotic Disease Transmission among Urban and Peri-urban Dairy Farmers in Ethiopia
Authors: Deneke Tilaye Teklewold , Bekele Adam , Moore Henrietta L. , Mamo Tadele , Almaw Gizat , Mekonnen Getnet Abie , Mihret Adane , Tschopp Rea , Yeheyis Likawent , Hodge Catherine , Wood James L.N. , Berg Stefan , consortium the ETHICOBOTS 
Published: September 2021

Abstract
Background: In the Ethiopian dairy farming system, prevalence of zoonotic diseases such as bovine tuberculosis (bTB) is high in the cattle population. This, combined with some risky milk and meat consumption habits, such as raw milk and uninspected raw meat consumption, poses a considerable risk of zoonotic disease transmission. A survey was conducted to investigate milk and meat consumption patterns, and the level of exposure to urban and peri-urban dairy-keeping households for risks of zoonotic disease transmission.Methods: Data on milk and meat consumption behaviours and other socioeconomic and demographic variables were collected from 480 urban and peri-urban dairy farms randomly surveyed in major towns in Ethiopia (Mekele, Hawassa, and Gondar towns, Addis Ababa city, as well as five Oromia towns around Addis Ababa). Determinants of raw milk consumption associated with a number of demographic and socio-economic factors were analysed using a generalized ordered logistic model.Results: The results indicated that about 20% the population consumed raw milk and their awareness about pasteurization and its benefits were low. Location, gender of the household head, previous bTB testing of cattle on the farm, knowledge of zoonotic risks associated with raw milk consumption, household size, and per-capita milk consumption were found to be important determinants of the frequency of raw milk consumption. About 60% of the respondents were exposed to the risk of zoonotic diseases through their habit of frequently consuming raw meat. This was depite that over 90% of the respondants were aware of possible zoonotic risks of raw meat consumption. The determinants of raw meat consumption behaviours were associated with location, gender and age of the household head, household size, meat type preference, per-capita meat consumption, knowledge about disease transmission risks, and training on zoonoses. Conclusion: Creating awareness about the risk factors for zoonotic transmission of diseases including bTB through training and media campaigns, improving meat hygiene through better abattoir services, and inducing behavioural change around meat sourcing, raw meat and raw milk consumption, are all crucial to the successful prevention and control of the spread of zoonotic diseases, including bTB.
An Association of Quality of Life and Ageing Perceptions Among Community Dwelling Older Adults in Uganda
Authors: -Onoria Joy Louise , Odokonyero Raymond , Giordani Bruno , Akena Dickens , Mwesiga Emmanuel , Ssuna Bashir , Ray Stuart C. , Bollinger Robert C. , Sewankambo Nelson K. , Nakasujja Noeline 
Published: September 2021

Abstract
Background: Uganda’s population, though, largely characterized by young people, has seen the number of people aged 60 and over grow from 686,000 twenty years ago, to 1,433,596 in 2014. Effective caring for the well-being of this population requires strategic and deliberate planning that involves quality of life (QoL) assessments. QoL assessments among the elderly are important in evaluating the efficacy of strategies, such as health interventions, welfare programs, health care, and well-being of the elderly. However, elderly in Uganda face several challenges, ranging from loneliness, poor housing, lack of social and financial support, and poor health. These may negatively affect older persons’ quality of life and consequently their perceptions and attitudes towards aging. Methods: The study was carried out in 2019 in the communities of Nansana and Busukuma town councils in Wakiso district, Uganda. The participants were 380 people 60 years and older. To establish the association between perceptions of ageing and QoL, this study utilized a locally adapted version of the Older Person’s Quality of Life Questionnaire (OPQOL) and the Brief Ageing Perceptions Questionnaire (B-APQ). The OPQOL assesses three domains of QoL: Health QoL (HQoL); Social economic QoL (SQoL); and Psychosocial QoL (PQoL). The B-APQ assesses perceptions about physical age, participation in social activities, and perceptions about ability to regulate emotions as one ages. Pearson’s Chi-square tests were used to characterize the relationship between the perceptions and quality of life.Results: The majority of the respondents, 61% (95%CI 56.7-64.8), had negative perceptions towards ageing. Eighty six percent had poor HQoL, 90% poor SQoL and 83% poor PQoL. There was a significant association between good HQoL and positive perception about participation in social activities (X2 = 7.3670, P = 0.007) as well as with positive perception on regulation of emotions (X2 = 18.1803, P<0.001). There was a significant association between good SQoL and positive perception about participation in social activities (X2 = 5.3472, P = 0.021), as well with positive perception on regulation of emotions (X2 = 10.5128, P<0.001). A significant association between good PQoL and positive perception on regulation of emotions (X2 = 9.2414, P= 0.002).Conclusion: Positive perceptions of ageing are associated with good QoL. Directly addressing perceptions of ageing could be a low cost and effective strategy to improve the QoL of older persons in SSA
Pulse-picking coherent anti-Stokes Raman scattering microscopy for highly sensitive chemical imaging
Authors: Clark Matthew , Gonzalez Gil , Zhang Chi 
Published: September 2021

Abstract
Coherent Raman scattering has been developed into powerful technologies for the chemical imaging of biological samples. However, limited sensitivity remains a critical bottleneck for coherent Raman microscopes. We introduce a pulse-picking technology that can significantly increase the sensitivity of coherent anti-Stokes Raman scattering (CARS) microscopy. An acousto-optic modulator driven by a function generator allows picking collinearly combined pump and Stokes pulses at low duty cycles. This method reduces the number of pulses at each image pixel, enhances the peak power of laser pulses, and thus significantly improves the sensitivity of CARS and minimizes phototoxicity. We demonstrated an over 1000-fold sensitivity improvement for CARS imaging. Besides, we show this pulse-picking method can enhance the sensitivity of multiphoton fluorescence and second harmonic generation modalities about 20 times. Using cell and biological tissue samples, we highlight the potential of this pulse-picking technology for highly sensitive multimodal chemical imaging in biological and medical sciences.
Is the Femoral Component Flexion Affected by the Sagittal Femoral Shaft Bowing in Conventional Intramedually Guided TKA?
Authors: Zhang Xiaofeng , Wang Qianjin , Xu Xingquan , Chen Dongyang , Bao Zhengyuan , Yao Yao , Wu Dengxian , Wang Bin , Xu Zhihong , Jiang Qing 
Published: September 2021

Abstract
Background: The aim of the present study was to investigate the influence of sagittal femoral bowing on sagittal femoral component alignment, and whether there was correlation between sagittal femoral component alignment and coronal femoral component alignment.Methods: We retrospectively reviewed 77 knees in 71 patients who had undergone primary TKA for advanced osteoarthritis.All surgeries were performed by using a standard medial parapatellar approach. The osteotomy was performed with a conventional technique using an intramedullar rod for the femur and a mechanical extramedullar guiding system for the tibia. All patients enrolled in the study were evaluated with full-length lower extremity load-bearing standing scanograms and the patients had preoperative and postoperative radiographs of the knees. cFBA(coronal femoral bowing angle), sFBA(sagittal femoral bowing angle),and postoperatively, mTFA(mechanical tibiofemoral angle of the knee), β angle(femoral component flexion angle) were measured. The radiographic results of both groups were compared using Student's t test. A two-sided Pearson correlation coefficient was obtained to identify the correlations between FBA in the coronal and sagittal planes, as well as FBA and age or BMI, sFBA and β angle, cFBA and mTFA. Comparison of FSB incidence between different genders was using chi-square test. The p value <0.05 indicates a statistically significant difference.Results: The mean sFBA, cFBA, β angle, mTFA were 9.34°±3.56°(range 1°-16°), 3.25°±3.79°(range -7°-17°), 3.91°±3.15°(range -1°-13°), 0.60°±1.95°(range -3°-6°), respectively. There was no correlation between age and sFBA(CC=0.192, p=0.194) or cFBA(CC=0.192, p=0.194), similarly, there was no correlation between age and sFBA(CC=0.067, p=0.565) or cFBA(CC=0.069, p=0.549). The sFBA was correlated with cFBA and β angle(CC=0.540, p<0.01; CC=0.543, p<0.01; respectively) and the cFBA was correlated with mTFA(CC=0.430, p<0.01). There was no significant difference(p=0.247) of cFBA between the patients with sFSB and the patients without sFSB. Conclusions: The current study showed that the sFBA was correlated with cFBA in the patients undergoing TKA and the patients with sFSB usually presented non-cFSB. We also found that sFSB could affect the femoral component alignment in the sagittal plane and cFSB could affect the femoral component alignment in the coronal plane. The sFBA or cFBA was not correlated with age, BMI or gender.
Anatomy of the Greater Occipital Nerve: Implications in Posterior Fossa Approaches.
Authors: LAINE Gaetan , JECKO Vincent , WAVASSEUR Thomas , GIMBERT Edouard , VIGNES Jean Rodolphe , LIGUORO Dominique 
Published: September 2021

Abstract
Purpose: Because of its superficial location in the dorsal regions of the scalp, the greater occipital nerve (GON) can be injured during neurosurgical procedures, resulting in post-operative pain and postural disturbances. The aim of this work is to specify the course of the GON and how its injuries can be avoided while performing posterior fossa approaches.Methods: This study was carried out at the department of anatomy in Bordeaux University. 4 specimens were dissected to study the GON course. Posterior fossa approaches (midline suboccipital, paramedian suboccipital, retrosigmoid and petrosal) were performed on 4 other specimens in order to assess potential risks of GON injuries.Results: The GON runs around the obliquus capitis inferior (100%), crosses the semispinalis capitis (100%) and the trapezius (75%) or its aponeurosis (25%). Direct GON injuries can be seen in paramedian suboccipital approaches. Stretching of the GON can occur in midline suboccipital and paramedian suboccipital approaches. We found no evidence of direct or indirect GON injury in retrosigmoid or petrosal approaches.Conclusion: Our study provides interesting data regarding the risk GON injury in posterior fossa approaches. Direct GON injuries in paramedian suboccipital approaches can be avoided with careful dissection. Placing retractors in contact with the periosteum and performing a minimal retraction may help to avoid excessive GON stretching in midline suboccipital and paramedian suboccipital approaches. Furthermore, incision for retrosigmoid approaches should be as lateral as possible and not too caudal. Finally, avoiding extreme patient positioning reduces the risk of GON stretching in all approaches.
Spatiotemporal Prediction of the Occurrence of Vancomycin-resistant Enterococcus
Authors: Niekerk J. M. van , Lokate M. , Braakman-Jansen L. M. A. , Gemert-Pijnen J. E. W. C. van , Stein A. 
Published: September 2021

Abstract
Background: Vancomycin-resistant enterococci (VRE) is the cause of severe patient health and monetary burdens. Antibiotic use is a confounding effect to predict VRE in patients, but the antibiotic use of patients who may have frequented the same ward as the patient in question is often neglected. This study investigated how the occurrence and spread of VRE can be explained by patient movements between hospital wards and their antibiotic use.Methods: Intrahospital patient movements, antibiotic use and PCR screening data were used from a hospital in the Netherlands. The PageRank algorithm was used to calculate two daily centrality measures based on the spatiotemporal graph to summarise the flow of patients and antibiotics at the ward level. A decision tree model was used to determine a simple set of rules to estimate the daily probability of VRE occurrence for each hospital ward. The model performance was improved using a random forest model and compared using 30% test sample.Results: Centrality covariates summarising the flow of patients and their antibiotic use between hospital wards can be used to predict the daily occurrence of VRE at the hospital ward level. The decision tree model produced a simple set of rules that can be used to determine the daily probability of VRE occurrence for each hospital ward. An acceptable area under the ROC curve (AUC) of 0.755 was achieved using the decision tree model and an excellent AUC of 0.883 by the random forest model on the test set. These results confirms that the random forest model performs better than a single decision tree for all levels of model sensitivity and specificity on data not used to estimate the models.Conclusion: This study showed how the movements of patients inside hospitals and their use of antibiotics could predict VRE occurrence at the ward level. Two daily centrality measures were proposed to summarise the flow of patients and antibiotics at the ward level. An early warning system for VRE can be developed to test and further develop infection prevention plans and outbreak strategies using these results.
Knockdown of microRNA-126 Suppresses Non-Small-Cell Lung Cancer via Inhibiting PI3K-AKT-mTOR Pathway
Authors: Chen Panfeng , Jiang Ping , Yu Xi , Yang Min 
Published: September 2021

Abstract
Background

As one of the most common cause of cancer death in the world, lung cancer causes approximate 1.6 million deaths annually. Among, NSCLC accounts approximately 85% of patients in whole lung cancer patients. As one of miRNAs, miR-126 closely involves in pathogenesis of several types of cancers including colorectal, prostate, bladder and gastric cancer, and so on. Thus, the present study aims to investigate effects of miR126 on pathogenesis of NSCLC. In the study, two lung cancer cell lines including A549 and H1650 were used.
Results

It was found that expression of miR126 was decreased in PBMC of NSCLC patients compared to healthy control. Expression of miR126 was decreased in cancer tissue compared to paracancerous tissues in NSCLC patients. MiR-126 KD remarkably increased expression of apoptosis genes including caspase3 and capsae9, and decreased cell viability in lung cancer cells including A549 and H1650 cells. Interesting, In Silico analysis indicated that miR-126 could target PI3K signaling pathway, which was confirmed by WB assay. KD of PI3KR2 compromised promotion of miR-126 on cell apoptosis. Similarly, it was found that KD of mTOR compromised promotion of miR-126 on cell apoptosis.
Conclusions

Thus, the present study confirmed that miR-126 plays an important role in apoptosis of lung cells via mTOR signaling pathway. miR-126 might be a potential therapeutic target for developing novel drugs against NSCLC.
Feasibility Analysis and Advantages of Wedge Resection Plus Transverse Suture Applied to Closure of Loop Ileostomy
Authors: Qin Hai-Quan , Liao Jian-Kun , Wang Wen-Tao , Meng Ling-Hou , Huang Zi-Gao , Mo Xian-Wei 
Published: September 2021

Abstract
Objectives: To evaluate the feasibility and advantages of wedge resection plus transverse suture applied to loop ileostomy closure by analyzing the surgical data and the incidence of postoperative complications of patients undergoing this procedure.Methods: We performed a retrospective analysis of the hospitalization data of patients who underwent ileostomy closure surgery and met the research standards from January 2017 to April 2021 in the Guangxi Medical University Cancer Hospital; all surgeries were performed by the same surgeon. The perioperative data were statistically analyzed by grouping.Results: In total, 65 patients were enrolled in this study, with 12 in the wedge resection group, 35 in the stapler group, and 18 in the hand suture group. There was no significant difference in operation time between the wedge resection group and stapler group (P > 0.05), but both groups had shorter operation time than that in the hand suture group (P< 0.05). The postoperative exhaustion time of wedge resection group was earlier than that of the others, and cost of surgical consumables in the wedge resection group was significantly lower than that in the stapler group, all with statistically significant differences (P< 0.05). By contrast, there was no statistically significant difference in postoperative complication incidences among the three groups.Conclusions: The wedge resection plus transverse suture is safe and easy for closure of loop ileostomy, and the intestinal motility recovers rapidly postoperatively. It has less surgical consumables, and is particularly suitable for the currently implemented Diagnosis-Related Groups payment method.