bluuebunny/crossref_metadata_2025_split huggingface dataset:
Serum renin and major adverse kidney events in critically ill patients: a multicenter prospective study
Authors: Flannery Alexander H. , Ortiz-Soriano Victor , Li Xilong , Gianella Fabiola G. , Toto Robert D. , Moe Orson W. , Devarajan Prasad , Goldstein Stuart L. , Neyra Javier A. 
Published: August 2021

Abstract
Background
Preliminary studies have suggested that the renin-angiotensin system is activated in critical illness and associated with mortality and kidney outcomes. We sought to assess in a larger, multicenter study the relationship between serum renin and Major Adverse Kidney Events (MAKE) in intensive care unit (ICU) patients.

Methods
Prospective, multicenter study at two institutions of patients with and without acute kidney injury (AKI). Blood samples were collected for renin measurement a median of 2 days into the index ICU admission and 5–7 days later. The primary outcome was MAKE at hospital discharge, a composite of mortality, kidney replacement therapy, or reduced estimated glomerular filtration rate to ≤ 75% of baseline.

Results
Patients in the highest renin tertile were more severely ill overall, including more AKI, vasopressor-dependence, and severity of illness. MAKE were significantly greater in the highest renin tertile compared to the first and second tertiles. In multivariable logistic regression, this initial measurement of renin remained significantly associated with both MAKE as well as the individual component of mortality. The association of renin with MAKE in survivors was not statistically significant. Renin measurements at the second time point were also higher in patients with MAKE. The trajectory of the renin measurements between time 1 and 2 was distinct when comparing death versus survival, but not when comparing MAKE versus those without.

Conclusions
In a broad cohort of critically ill patients, serum renin measured early in the ICU admission is associated with MAKE at discharge, particularly mortality.
Quantifying the phase separation property of chromatin-associated proteins under physiological conditions using an anti-1,6-hexanediol index
Authors: Shi Minglei , You Kaiqiang , Chen Taoyu , Hou Chao , Liang Zhengyu , Liu Mingwei , Wang Jifeng , Wei Taotao , Qin Jun , Chen Yang , Zhang Michael Q. , Li Tingting 
Published: August 2021

Background
Liquid-liquid phase separation (LLPS) is an important organizing principle for biomolecular condensation and chromosome compartmentalization. However, while many proteins have been reported to undergo LLPS, quantitative and global analysis of chromatin LLPS property remains absent.

Results
Here, by combining chromatin-associated protein pull-down, quantitative proteomics and 1,6-hexanediol (1,6-HD) treatment, we develop Hi-MS and define an anti-1,6-HD index of chromatin-associated proteins (AICAP) to quantify 1,6-HD sensitivity of chromatin-associated proteins under physiological conditions. Compared with known physicochemical properties involved in phase separation, we find that proteins with lower AICAP are associated with higher content of disordered regions, higher hydrophobic residue preference, higher mobility and higher predicted LLPS potential. We also construct BL-Hi-C libraries following 1,6-HD treatment to study the sensitivity of chromatin conformation to 1,6-HD treatment. We find that the active chromatin and high-order structures, as well as the proteins enriched in corresponding regions, are more sensitive to 1,6-HD treatment.

Conclusions
Our work provides a global quantitative measurement of LLPS properties of chromatin-associated proteins and higher-order chromatin structure. Hi-MS and AICAP data provide an experimental tool and quantitative resources valuable for future studies of biomolecular condensates.
CPB-3 and CGH-1 localize to motile particles within dendrites in C. elegans PVD sensory neurons
Authors: Spendier Kathrin , Olesnicky Eugenia C. , Forand Daniel , Wolf Margaret , Killian Darrell J. 
Published: August 2021

AbstractObjectiveRNA-binding proteins (RBPs) are important regulators of gene expression that influence mRNA splicing, stability, localization, transport, and translational control. In particular, RBPs play an important role in neurons, which have a complex morphology. Previously, we showed that there are many RBPs that play a conserved role in dendrite development inDrosophiladendritic arborization neurons andCaenorhabditis elegans(C. elegans) PVD neurons including the cytoplasmic polyadenylation element binding proteins (CPEBs), Orb inDrosophilaand CPB-3 inC. elegans, and the DEAD box RNA helicases, Me31B inDrosophilaand CGH-1 inC. elegans. During these studies, we observed that fluorescently-labeled CPB-3 and CGH-1 localize to cytoplasmic particles that are motile, and our research aims to further characterize these RBP-containing particles in live neurons.ResultsHere we extend on previous work to show that CPB-3 and CGH-1 localize to motile particles within dendrites that move at a speed consistent with microtubule-based transport. This is consistent with a model in which CPB-3 and CGH-1 influence dendrite development through the transport and localization of their mRNA targets. Moreover, CPB-3 and CGH-1 rarely localize to the same particles suggesting that these RBPs function in discrete ribonucleoprotein particles (RNPs) that may regulate distinct mRNAs.
Young adult with Gorham’s disease presenting in an emergency department: a case report
Authors: Grönroos Markku , Palomäki Ari 
Published: August 2021

Abstract
Introduction
Gorham’s disease is a very rare musculoskeletal disorder characterized by progressive resorption of one or more skeletal bones. Most of the 200 cases reported earlier are diagnosed before the age of 40 years. Due to rarity, the diagnosis of Gorham’s disease in the Emergency Department may be very difficult.

Case presentation
We report a case of Gorham’s disease. A 23-year old Caucasian man presented to the Emergency Department with a significant loss of power and sensation of the lower limbs and lower torso. Clinical examination, computed tomography, and magnetic resonance imaging revealed resorption of the ribs and vertebrae, severe kyphosis, and spinal stenosis in the thoracic area. The patient underwent several surgical procedures, including spondylodesis and decompression, and made a good initial recovery. Biopsy confirmed the diagnosis of Gorham’s disease.

Conclusion
We present a young man with Gorham’s disease visiting the Emergency Department. After the proper diagnosis and treatment, our patient had good outcome, although the etiology of this rare disease is uncertain.
Towards sustainable energy culture in the industrial sector: introducing an interdisciplinary method for understanding energy culture in business industries
Authors: Oksman Virpi , Reda Francesco , Karjalainen Sami , Rehman Hassam ur , Fatima Zarrin 
Published: August 2021

Abstract
Background
There have been numerous research papers focusing on improving energy consumption and energy behaviour in domestic and residential contexts. However, workplaces and especially industry settings have gained less attention in scientific literature, even though the industry sector is one of the largest energy consumers in the world. This article introduces a methodological framework that utilizes the energy culture concept, to support understanding the factors that influence energy culture at business industry companies.

Methods
Building on the concept of energy culture, we introduce an interdisciplinary method, which assesses organizations’ energy culture from different perspectives and recognizes the possibilities for sustainability transitions. To validate this method, the developed energy culture survey has been verified by 27 expert participants from different industry-related companies located in Finland, Italy, Switzerland, Germany France, and Austria.

Results
Our analysis highlights the need to consider diverse, interdisciplinary aspects to create a successful method for enhancing energy culture in the industrial sector. This will take into consideration human aspects, related to cognitive norms, beliefs, and aspirations, as well as to human interaction with the material world.

Conclusions
Industrial energy cultures context differs from domestic and residential contexts, and the knowledge from one context cannot be transferred to another context as such. Based on investigated studies undertaken for residential, office, and other sectors and the lessons learned, we developed a systematic method for energy culture understanding in industries. Energy managers may use it, as well as other individuals involved in energy culture issues in the industrial business sector, to evaluate the state of energy culture and engage employees towards new energy-related practices.
Dickkopf-related protein 3 alters aerobic glycolysis in pancreatic cancer BxPC-3 cells, promoting CD4+ T-cell activation and function
Authors: Guo Qingqu , Chu Yiming , Li Hongbo , Shi Dike , Lin Lele , Lan Weifeng , Wu Dan 
Published: August 2021

Abstract
Background
To investigate the value of Dickkopf-related protein 3 (DKK3) on aerobic glycolysis in pancreatic cancer cells, where DKK3-overexpression is used to determine its effects on CD4+ T cells.

Methods
The BxPC-3-DKK3 cell line was constructed, and peripheral blood mononuclear cell (PBMC) was prepared. After isolated the CD4+ T cells, the lactic acid, glucose uptake ability, cellular viability, proliferation, apoptosis, and markers were detected by PCR and western blot, and the concentrations of multiple cytokines were determined using the ELISA method.

Results
After co-culture with pancreatic cancer cells overexpressing DKK3, the glucose uptake markedly, proliferation enhanced and apoptosis inhibited in CD4+ T cells. The co-culture model also revealed that DKK3-overexpression promotes the activation and regulates the metabolism and function of CD4+ T cells.

Conclusions
DKK3 alters the metabolic microenvironment of pancreatic cancer cells and further facilitates the function of CD4+
T cells which suggesting that DKK3 may have a therapeutic potential in pancreatic cancer.
What characterizes a good mental health professional in court-mandated treatment settings?: Findings from a qualitative study with older patients and mental health care professionals
Authors: Seaward Helene , Wangmo Tenzin , Vogel Tobias , Graf Marc , Egli-Alge Monika , Liebrenz Michael , Elger Bernice S. 
Published: August 2021

Abstract
Background
Therapist-related activities and characteristics such as empathy and genuineness are factors that significantly contribute to psychotherapy outcome. As they play a role in psychotherapy more generally, it can be expected that they are equally important in the treatment of court-mandated patients more specifically. At the same time, these treatment settings come with specific challenges—e.g. due to coercion and control—and it could thus be that some therapist-related characteristics might have a different empathy on the therapy. This interview study sought to investigate service providers’ and users’ perspectives on therapist-related characteristics in the context of detention.

Methods
We conducted a qualitative interview study with 41 older incarcerated persons mandated to treatment, and 63 mental health professionals (MHP). The data analysis followed thematic analysis.

Results
Patients and experts both emphasized the importance of treating patients with respect by taking a humanistic approach, that is, condemn the deeds but embrace the person and display genuine interest in supporting patients with any issue or concern that is of relevance to them. Furthermore, interviewees underscored that the coerciveness of the therapy context required to incorporate patients’ wishes into treatment planning, recognize and respond to the patients’ needs, and allow some choice within the given framework. Such inclusive attitude was deemed critical to engage and motivate patients to participate in treatment. In addition, it was emphasized that feedback and advice by the therapists need to be concrete, detailed and applied to each person’s current situation. Lastly, patients questioned MHP’s qualification when they did not progress in therapy.

Discussion
Our findings indicate that some therapist-related activities and characteristics are of particular importance in court-mandated settings. These include genuine interest in the patient, a respectful and positive attitude, as well as the capacity to target sensitive issues in a directive but non-confrontational manner. Further research needs to identify specific expressions and behaviors that are linked to the aforementioned characteristics in the forensic context. Our study therefore contributes to much-needed empirical research on clinician and patient perspectives on therapist characteristics and activities in the treatment of court-mandated patients.
North Sea spring bloom-associated Gammaproteobacteria fill diverse heterotrophic niches
Authors: Francis Ben , Urich Tim , Mikolasch Annett , Teeling Hanno , Amann Rudolf 
Published: August 2021

AbstractBackgroundThe planktonic bacterial community associated with spring phytoplankton blooms in the North Sea is responsible for a large amount of carbon turnover in an environment characterised by high primary productivity. Individual clades belonging to theGammaproteobacteriahave shown similar population dynamics toBacteroidetes species, and are thus assumed to fill competing ecological niches. Previous studies have generated large numbers of metagenome assembled genomes and metaproteomes from these environments, which can be readily mined to identify populations performing potentially important ecosystem functions. In this study we attempt to catalogue these spring bloom-associatedGammaproteobacteria, which have thus far attracted less attention than sympatricAlphaproteobacteriaandBacteroidetes.MethodsWe annotated 120 non-redundant species-representative gammaproteobacterial metagenome assembled genomes from spring bloom sampling campaigns covering the four years 2010–2012 and 2016 using a combination of Prokka and PfamScan, with further confirmation via BLAST against NCBI-NR. We also matched these gene annotations to 20 previously published metaproteomes covering those sampling periods plus the spring of 2009.ResultsMetagenome assembled genomes with clear capacity for polysaccharide degradation via dedicated clusters of carbohydrate active enzymes were among the most abundant during blooms. Many genomes lacked gene clusters with clearly identifiable predicted polysaccharide substrates, although abundantly expressed loci for the uptake of large molecules were identified in metaproteomes. While the larger biopolymers, which are the most abundant sources of reduced carbon following algal blooms, are likely the main energy source, some gammaproteobacterial clades were clearly specialised for smaller organic compounds. Their substrates range from amino acids, monosaccharides, and DMSP, to the less expected, such as terpenoids, and aromatics and biphenyls, as well as many ‘unknowns’. In particular we uncover a much greater breadth of apparent methylotrophic capability than heretofore identified, present in several order level clades without cultivated representatives.ConclusionsLarge numbers of metagenome assembled genomes are today publicly available, containing a wealth of readily accessible information. Here we identified a variety of predicted metabolisms of interest, which include diverse potential heterotrophic niches of spring bloom-associatedGammaproteobacteria. Features such as those identified here could well be fertile ground for future experimental studies.
Modulating the bicoid gradient in space and time
Authors: Cai Xiaoli , Rondeel Inge , Baumgartner Stefan 
Published: August 2021

AbstractBackgroundThe formation of the Bicoid (Bcd) gradient in the earlyDrosophilais one of the most fascinating observations in biology and serves as a paradigm for gradient formation, yet its mechanism is still not fully understood. Two distinct models were proposed in the past, the SDD and the ARTS model.ResultsWe define novelcis- andtrans-acting factors that are indispensable for gradient formation. The first one is the poly A tail length of thebcdmRNA where we demonstrate that it changes not only in time, but also in space. We show that posteriorbcdmRNAs possess a longer poly tail than anterior ones and this elongation is likely mediated bywispy(wisp), a poly A polymerase. Consequently, modulating the activity of Wisp results in changes of the Bcd gradient, in controlling downstream targets such as the gap and pair-rule genes, and also in influencing the cuticular pattern. Attempts to modulate the Bcd gradient by subjecting the egg to an extra nuclear cycle, i.e. a 15thnuclear cycle by means of thematernal haploid(mh) mutation showed no effect, neither on the appearance of the gradient nor on the control of downstream target. This suggests that the segmental anlagen are determined during the first 14 nuclear cycles. Finally, we identify theCyclin B(CycB) gene as atrans-acting factor that modulates the movement of Bcd such that Bcd movement is allowed to move through the interior of the egg.ConclusionsOur analysis demonstrates that Bcd gradient formation is far more complex than previously thought requiring a revision of the models of how the gradient is formed.
No association between rheumatoid arthritis and cognitive impairment in a cross-sectional national sample of older U.S. adults
Authors: Booth Michael J. , Janevic Mary R. , Kobayashi Lindsay C. , Clauw Daniel J. , Piette John D. 
Published: August 2021

Abstract
Background
Studies suggest an increased prevalence of cognitive impairment (CI) among people with rheumatoid arthritis (RA). However, most prior studies have used convenience samples which are subject to selection biases or have failed to adjust for key confounding variables. We thus examined the association between CI and RA in a large national probability sample of older US adults.

Methods
Data were from interviews with 4462 participants in the 2016 wave of the nationally representative U.S. Health and Retirement Study with linked Medicare claims. RA diagnoses were identified via a minimum of two ICD-9CM or ICD-10 codes in Medicare billing records during the prior 2 years. The Langa-Weir Classification was used to classify cognitive status as normal, cognitively impaired non-dementia (CIND), or dementia based on a brief neuropsychological battery for self-respondents and informant reports for proxy respondents. We compared the odds of CI between older adults with and without RA using logistic regression, adjusted for age, education, gender, and race.

Results
Medicare records identified a 3.36% prevalence of RA (150/4462). While age, gender, education, and race independently predicted CI status, controlling for these covariates we found no difference in CI prevalence according to RA status (prevalent CI in 36.7% of older adults with RA vs. 34.0% without RA; adjusted OR = 1.08, 95% CI 0.74–1.59, p = .69).

Conclusion
There was no association between RA and CI in this national sample of older U.S. adults.
Effects of ante-mortem use of methadone on insect succession patterns
Authors: Keshavarzi Davoud , Rassi Yavar , Oshaghi Mohamad Ali , Azizi Korush , Rafizadeh Sayena , Alimohammadi Alimohammad , Parkhideh Seyed Zahra 
Published: August 2021

AbstractBackgroundEvaluation of insect succession patterns is a scientific method to estimate the time elapsed since death. Several studies have shown that ante-mortem intake of opioids affect maggot growth rate. However, there are few published data that investigate the effect of ante-mortem opioid use on insect succession patterns.Therefore, the main purpose of this research was to investigate the effect of methadone on the succession patterns of insects on rabbit carcasses during the spring and winter of 2019. In the present study, the H-null hypothesis represents the dissimilarity between the successional waves of species from testing and control carcasses (H0: P: 0).ResultsDuring this study, 15 and 13 insect species were collected from carcasses during the spring and winter, respectively. The most dominant species during the both seasons wereChrysomya albicepsandCalliphora vicina. These two species preferred to lay eggs on the control carcasses earlier than the treated carcasses.Lucilia cuprinawas observed only from the remains of untreated carcasses, whileSaprinus chalcitesandNecrobia rufipeswere recorded only from the remains of treated rabbits. Samples indicate that 11.8% of the insects were members of the Coleoptera. Permutation analyzes based on the Mantel test were 0.647 ± 0.16 (P= 0.009) and 0.693 ± 0.16 (P= 0.003) for the similarity of the species between treated and untreated carcasses in the spring and winter, respectively. Permutation analyzes for the two most dominant fly species (Ch. albicepsandC. vicina) between the treated and untreated carcasses in the spring and winter were 0.515 ± 0.15 (P= 0.05) and 0.491 ± 0.14 (P= 0.09), respectively.ConclusionThe results revealed that the overall pattern of insect succession was similar between the treated and untreated rabbit carcasses. However, the patterns of succession ofChrysomya albicepsandCalliphora vicinadiffered slightly between treated and untreated carcasses, and this could have an effect on the PMI min estimate.
Bone remodeling and implant migration of uncemented femoral and cemented asymmetrical tibial components in total knee arthroplasty - DXA and RSA evaluation with 2-year follow up
Authors: Yilmaz Müjgan , Holm Christina Enciso , Lind Thomas , Flivik Gunnar , Odgaard Anders , Petersen Michael Mørk 
Published: August 2021

Abstract
Background
Aseptic loosening is one of the major reasons for late revision in total knee arthroplasty (TKA). The risk of aseptic loosening can be detected using radiostereometric analysis (RSA), whereby micromovements (migration) can be measured, and thus RSA is recommended in the phased introduction of orthopedic implants. Decrease in bone mineral density (BMD), as measured by dual-energy x ray absorptiometry (DXA), is related to the breaking strength of the bone, which is measured concurrently by RSA. The aim of the study was to evaluate bone remodeling and implant migration with cemented asymmetrical tibial and uncemented femoral components after TKA with a follow up period of 2 years.

Methods
This was a prospective longitudinal cohort study of 29 patients (number of female/male patients 17/12, mean age 65.2 years), received a hybrid Persona® TKA (Zimmer Biomet, Warsaw, IN, USA) consisting of a cemented tibial, an all-polyethylene patella, and uncemented trabecular metal femoral components. Follow up: preoperative, 1 week, and 3, 6, 12 and 24 months after surgery, and double examinations for RSA and DXA were performed at 12 months. RSA results were presented as maximal total point of motion (MTPM) and segmental motion (translation and rotation), and DXA results were presented as changes in BMD in different regions of interest (ROI).

Results
MTPM at 3, 6, 12, and 24 months was 0.65 mm, 0.84 mm, 0.92 mm, and 0.96 mm for the femoral component and 0.54 mm, 0.60 mm, 0.64 mm, and 0.68 mm, respectively, for the tibial component. The highest MTPM occurred within the first 3 months. Afterwards most of the curves flattened and stabilized. Between 12 and 24 months after surgery, 16% of femoral components had migrated by more than 0.10 mm and 15% of tibial components had migrated by more than 0.2 mm. Percentage change in BMD in each ROI for distal femur was as follows: ROI I 26.7%, ROI II 9.2% and ROI III 3.3%. BMD and at the proximal tibia: ROI I 8.2%, ROI II 8.6% and ROI III 7.0% after 2 years compared with 1 week postoperative results. There was no significant correlation between maximal percentwise change in BMD and MTPM after 2 years.

Conclusion
Migration patterns and changes in BMD related to femoral components after TKA in our study correspond well with previous studies; we observed marginally greater migration with the tibial component.