bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluation of water, sanitation and hygiene status of COVID-19 healthcare facilities in Ghana using the WASH FIT approach
Authors: Ashinyo Mary Eyram , Amegah Kingsley E. , Dubik Stephen Dajaan , Ntow-Kummi Gloria , Adjei Maxwell Kudzo , Amponsah Joyce , Ayivase John T. , Amoah Serwah , Ashinyo Anthony , Sodzi-Tettey Sodzi , Awekeya Hectoria , Codjoe Agnes , Tegbey Isaac Eyram , Kwakye Akosua Takyiwah O. , Akoriyea Samuel Kaba 
Published: April 2021

Abstract
Access to improved water, sanitation and hygiene (WASH) is essential for the delivery of high-quality care in healthcare facilities and the prevention of hospital-acquired infections such as the transmission of the SARS-CoV-2. In addition, unimpeded access to WASH facilities in coronavirus disease 2019 (COVID-19) treatment centres (TCs) is central in facilitating compliance with infection prevention and control protocols. However, data for the WASH status of COVID-19 TCs in Ghana are limited. We evaluated the WASH status of seven COVID-19 TCs in Ghana using the WHO/UNICEF water and sanitation for health facility improvement tool (WASH FIT). The water domain had the highest number of indicators meeting standards with an average percentage score of 90.5% (range: 66.7–100%) across the seven TCs, followed by management (66.9%) and hygiene (58.7%). The TCs performed poorly in the sanitation and healthcare waste domain, with an average percentage score of 44.6% (range: 22.2–75%). These findings highlight the challenges being faced by COVID-19 TCs in implementing WASH services. Specific WASH interventions are urgently required to strengthen WASH services in the COVID-19 TCs. This should precede the prioritisation of resources to WASH infrastructure in the treatment facilities coupled with actions that involve all stakeholders.
The Differential Effects of Social Media on Depressive Symptoms and Suicidal Ideation Among the Younger and Older Adult Population in Hong Kong During the COVID-19 Pandemic: Population-Based Cross-sectional Survey Study
Authors: Yang Xue , Yip Benjamin H K , Mak Arthur D P , Zhang Dexing , Lee Eric K P , Wong Samuel Y S 
Published: April 2021

Background
Social media has become a ubiquitous part of daily life during the COVID-19 pandemic isolation. However, the role of social media use in depression and suicidal ideation of the general public remains unclear. Related empirical studies were limited and reported inconsistent findings. Little is known about the potential underlying mechanisms that may illustrate the relationship between social media use and depression and suicidal ideation during the COVID-19 pandemic.

Objective
This study tested the mediation effects of social loneliness and posttraumatic stress disorder (PTSD) symptoms on the relationship between social media use and depressive symptoms and suicidal ideation, as well as the moderation effect of age on the mediation models.

Methods
We administered a population-based random telephone survey in May and June 2020, when infection control measures were being vigorously implemented in Hong Kong. A total of 1070 adults (658 social media users and 412 nonusers) completed the survey. Structural equation modeling (SEM) and multigroup SEM were conducted to test the mediation and moderation effects.

Results
The weighted prevalence of probable depression was 11.6%; 1.6% had suicidal ideation in the past 2 weeks. Both moderated mediation models of depressive symptoms (χ262=335.3; P<.05; comparative fit index [CFI]=0.94; nonnormed fit index [NNFI]=0.92; root mean square error of approximation [RMSEA]=0.06) and suicidal ideation (χ234=50.8; P<.05; CFI=0.99; NNFI=0.99; RMSEA=0.02) showed acceptable model fit. There was a significantly negative direct effect of social media use on depressive symptoms among older people (β=–.07; P=.04) but not among younger people (β=.04; P=.55). The indirect effect via PTSD symptoms was significantly positive among both younger people (β=.09; P=.02) and older people (β=.10; P=.01). The indirect effect via social loneliness was significant among older people (β=–.01; P=.04) but not among younger people (β=.01; P=.31). The direct effect of social media use on suicidal ideation was not statistically significant in either age group (P>.05). The indirect effects via PTSD symptoms were statistically significant among younger people (β=.02; P=.04) and older people (β=.03; P=.01). Social loneliness was not a significant mediator between social media use and suicidal ideation among either age group (P>.05).

Conclusions
Social media may be a “double-edged sword” for psychosocial well-being during the COVID-19 pandemic, and its roles vary across age groups. The mediators identified in this study can be addressed by psychological interventions to prevent severe mental health problems during and after the COVID-19 pandemic.
A pilot study on exertional tasks with physiological measures designed for the assessment of military concussion
Authors: Prim Julianna H , Davila Maria I , McCulloch Karen L 
Published: April 2021

Background: Guidelines for clinicians treating military concussion recommend exertional testing before return-to-duty, yet there is currently no standardized task or inclusion of an objective physiological measure like heart rate variability (HRV). Methodology & results: We pilot-tested two clinically feasible exertional tasks that include HRV measures and examined reliability of a commercially available heart rate monitor. Testing healthy participants confirmed that the 6-min step test and 2-min pushup test evoked the targeted physiological response, and the Polar H10 was reliable to the gold-standard electrocardiogram. Conclusion: Both tasks are brief assessments that can be implemented into primary care setting including the Polar H10 as an affordable way to access HRV. Additional research utilizing these tasks to evaluate concussion recovery can validate standardized exertional tasks for clinical use.
Tradisi Ruwatan Jawa pada Masyarakat Desa Pulungdowo Malang
Authors: Susanti Jijah Tri , Lestari Dinna Eka Graha 
Published: April 2021

Jawa yang merupakan salah satu suku terbesar di Indonesia juga memiliki keanekaragaman budaya. Masyarakat Jawa kental dengan kepercayaan mistis atau sering disebut juga kepercayaan dalam dunia Spiritual. Masyarakat Jawa memiliki beragam teori yang menjadi dasar dilakukannya sebuah ritual. Upacara atau ritual yang dilakukan untuk menghindarkan diri dari dampak yang ditimbulkan akibat kesalahan manusia, dalam masyarakat Jawa disebut ruwatan. Tujuan penelitian ini adalah untuk mengetahui sejarah asal mula tradisi ruwatan adat Jawa bagi anak perempuan tunggal, mengetahui makna yang terkandung dalam tradisi ruwatan adat Jawa bagi anak perempuan tunggal, dan proses tradisi ruwatan adat Jawa bagi anak perempuan tunggal sebelum melakukan pernikahan. Metode penelitian ini adalah deskriptif kualitatif. Penelitian dilakukan di Desa Pulungdowo Kabupaten Malang. Sumber data penelitian ini adalah informan dan fenomena tradisi ruwatan. Teknik pengumpulan data dilakukan dengan observasi, wawancara dan studi dokumen. Teknik analisa data dilakukan dengan reduksi, interpetasi, dan penarikan kesimpulan. Hasil penelitian ini menunjukkan bahwa asal muasal adanya Ruwatan adalah dari cerita pewayangan ada seorang tokoh yang bernama Batara Guru dia beristrikan dua orang istri yaitu Pademi dan Selir. Ruwatan dilakukan dengan menggelar pertunjukan wayang lakon Murwakala. Dalang akan menyajikan sesaji khusus untuk memuja batarakala. Pada acara pamungkas dalang membaca mantra dengan iringan gamelan dan gending sebagai tolak bala (mengusir Batarakala). Makna dari ruwatan adalah meminta dengan sepenuh hati agar pelakunya lepas dari petaka dan memperoleh rahayu keselamatan. Hal tersebut memperlihatkan masih kuatnya kepercayaan masyarakat terhadap mitos dan tradisi utamanya bagi ruwatan anak perempuan tunggal sebelum melangsungkan pernikahan.
Histone demethylases JHDM1D, PHF2 and PHF8 expression pattern in granulosa cells obtained from patients undergoing IVF procedure during short-term IVC
Authors: Bryl Rut , Stefańska Katarzyna , Chermuła Błażej , Stelmach Bogumiła , Pieńkowski Wojciech , Kulus Jakub , Perek Joanna , Wieczorkiewicz Maria , Wąsiatycz Grzegorz , Ratajczak Kornel , Pawelczyk Leszek , Mozdziak Paul , Jeseta Michal , Spaczyński Robert Z. , Bukowska Dorota 
Published: April 2021

Abstract
Granulosa cells play an important role in follicle development, maturation, and atresia. They are a cellular source of the two most important ovarian steroids, namely, estradiol and progesterone and are also crucial for bidirectional communication with the oocyte, thus being involved in the regulation of its growth, development and function. Growing body of evidence suggests that granulosa cells cultured in vitro display stemness and transdifferentiation potential. Together with the fact that they can be easily collected during IVF procedures, these properties of GCs may be of particular interest for both regenerative medicine and transplantology. Establishment of in vitro cell culture and its thorough characterization, including molecular, is crucial for future potential utilization of human granulosa cells in design of engineered tissue grafts or cell-based therapies, in particular targeted at female infertility. Nevertheless, the transcriptomic alterations which may occur during in vitro culture of granulosa cells are still largely uncharacterized. The aim of this study was to examine expression changes of three genes encoding histone demethylases which serve as transcription coactivators in short term in vitro cell culture of human granulosa cells. The study groups consisted of 14 patients, aged 18–40 years undergoing in vitro fertilization (IVF). Expression level assessment was performed after 24 h, 48 h, 72 h, 96 h, 120 h, 144 h and 168 h of in vitro primary cell culture utilizing RT-qPCR technique. Upregulation of PHF2 expression in all time points of the culture was observed, whereas the tendency of JHDM1D and PHF8 was mainly to decrease in expression level. Further study on a larger population would be required in order to confirm the presented tendencies.

Running title: Expression pattern of selected histone demethylases in human granulosa cells
Increased transcript expression levels of DNA methyltransferases type 1 and 3A during cardiac muscle long-term cell culture
Authors: Nawrocki Mariusz J. , Bryl Rut , Kałużna Sandra , Stefańska Katarzyna , Stelmach Bogumiła , Jemielity Marek , Perek Bartłomiej , Bukowska Dorota , Mozdziak Paul , Petitte James N. , Kempisty Bartosz 
Published: April 2021

Abstract
Heart failure (HF) is one of the main causes of death worldwide. Recent studies reported altered DNA methylation in failing human hearts. This may suggest a role of DNA methylation, most frequently implicated in epigenetic control, in the development of heart failure. Here, employing RT-qPCR, we characterized transcript levels for main DNA methyltransferases (DNMTs), DNMT1, DNMT3A, and DNMT3B, mediate DNA methylation, and they have different functions that complement each other during methylation. All analyzes were performed at different stages of porcine myocardial cell primary culture. In the present study we demonstrated increasing transcript expression levels for all analyzed genes during in vitro cultivation. The changes for DNMT1 and DNMT3A seem to be particularly important, where statistically significant changes were observed.

Running title: DNMTs role in cardiac muscle cell culture
Piperazine sulfonamides as DPP-IV inhibitors: Synthesis, induced-fit docking and in vitro biological evaluation
Authors: Khalaf Reema Abu , Alwarafi Ebtisam , Sabbah Dima 
Published: April 2021

Abstract
Diabetes mellitus is a chronic illness that needs persistent medical attention and continuous patient self-management to avoid acute complications. Dipeptidyl peptidase-IV (DPP-IV) inhibitors minimize glucagon and blood glucose levels by increasing the incretin levels, glucagon-like peptide (GLP-1) and glucose-dependent insulinotropic poly-peptide (GIP), leading to insulin secretion from pancreatic beta cells. In the present study, nine 1,4-bis(phenylsulfonyl) piperazine derivatives 1a-i were synthesized and identified using 1H NMR, 13C NMR, MS and IR spectroscopies. These compounds were tested in vitro and showed inhibitory activity ranging from 11.2 to 22.6 % at 100 µmol L–1 concentration. Piperazine sulfonamide derivatives were found to be promising DPP-IV inhibitors, where the presence of electron-withdrawing groups such as Cl (1a-c) improved the activity of the compounds more than electron-donating groups such as CH3 (
1d-f) at the same position. Additionally, meta-substitution is disfavored (1b, 1e, 1g). Induced-fit docking studies suggested that the targeted compounds 1a-i occupy the binding domain of DPP-IV and form H-bonding with the backbones of R125, E205, E206, F357, K554, W629, Y631, Y662 and R669.
Determination of penicillamine, tiopronin and glutathione in pharmaceutical formulations by kinetic spectrophotometry
Authors: Kukoc-Modun Lea , Biocic Maja , Radić Njegomir 
Published: April 2021

Abstract
A novel and simple method for the determination of penicillamine (PEN), tiopronin (mercaptopropionyl glycine, MPG) and glutathione (GSH) in pharmaceutical formulations by kinetic spectrophotometry has been developed and validated. It is based on the redox reaction where the thiol compound (RSH) reduces CuII-neocuproine complex to CuI-neocuproine complex. The non-steady state signal of the formed CuI- neocuproine complex is measured at 458 nm. The initial rate and fixed time (at 1 min) methods were validated. The calibration graph was linear in the concentration range from 8.0 × 10‒7 to 8.0 × 10‒5 mol L−1 for the initial rate method and from 6.0 × 10‒7 to 6.0 × 10−5 mol L−1 for the fixed time method, with the detection limits of 2.4 × 10−7 and 1.4 × 10‒7 mol L−1, resp. Levels of PEN, MPG and GSH in pharmaceutical formulations were successfully assayed by both methods. The advantages of the presented methods include sensitivity, short analysis time, ease of application and low cost.
New derivatives of sulfonylhydrazone as potential antitumor agents: Design, synthesis and cheminformatics evaluation
Authors: Sabbah Dima A. , Al-Azaideh Bara’a A. , Talib Wamidh H. , Hajjo Rima , Sweidan Kamal , Al-Zuheiri Aya M. , Sheikha Ghassan Abu , Shraim Sawsan 
Published: April 2021

Abstract
Phosphoinositide 3-kinase α (PI3Kα) is a propitious target for designing anticancer drugs. A series of new N’-(diphenylmethylene)benzenesulfonohydrazide was synthesized and characterized using FT-IR, NMR (1H and 13C), HRMS, and elemental analysis. Target compounds exhibited an antiproliferative effect against the human colon carcinoma (HCT-116) cell line. Our cheminformatics analysis indicated that the para-tailored derivatives [p-NO2 (3) and p-CF3 (7)] have better ionization potentials based on calculated Moran autocorrelations and ionization potentials. Subsequent in vitro cell proliferation assays validated our cheminformatics results by providing experimental evidence that both derivatives 3 and 7 exhibited improved antiproliferative activities against HCT-116. Hence, our results emphasized the importance of electron-withdrawing groups and hydrogen bond-acceptors in the rational design of small-molecule chemical ligands targeting PI3Kα. These results agreed with the induced-fit docking against PI3Kα, highlighting the role of p-substituted aromatic rings in guiding the ligand-PI3Kα complex formation, by targeting a hydrophobic pocket in the ligand-binding site and forming π-stacking interactions with a nearby tryptophan residue.
Propolis – quality analysis and use in topical formulations
Authors: Ramanauskiene Kristina , Inkeniene Asta Marija , Perminaite Kristina , Zilius Modestas , Majiene Daiva 
Published: April 2021

Abstract
The aim of this study was to produce propolis extracts, assess their quality and effect on skin cells and determine the penetration of active ingredients from designed semi-solid topical formulations. The use of higher-concentration ethanol and a larger amount of raw material allows extracting a larger quantity of active ingredients from raw propolis. Ultrasound extraction is an effective method for the production of aqueous extracts of propolis. The results show that depending on concentration, propolis extracts reduce the viability of keratinocytes. The phenolic compounds under observation penetrated the epidermis and dermis from designed formulations. The base of semi-solid formulation influences the efficacy of propolis preparations. The overall quantity of phenolic compounds that penetrated the skin was around 2 % from the ointment and 1.5 % from the cream.
3D-Printed Tool Shoulder Design for the Analogue Modelling of Bobbin Friction Stir Weld Joint Quality
Authors: Tamadon A. , Pons D. J. , Chakradhar K. , Kamboj J. , Clucas D. 
Published: April 2021

Abstract
A variety of tool shoulder designs comprising three families i.e. blade, spiral and circular shaped scrolls, were produced to improve the material flow and restrictions to avoid the tunnel void. The bobbin tools were manufactured by 3D printing additive manufacturing technology using solid filament. The butt weld joint was produced by each tool using plasticine as the workpiece material. The apparent surface features and bi-colour cross-sections provided a physical flow comparison among the shoulder designs. For the bobbin friction stir welding (BFSW), the tool shoulder with a three-spiral design produced the most stability with the best combination of the flow patterns on surface and cross-sections. The circular family tools showed a suitable intermixing on the surface pattern, while the blade scrolls showed better flow features within the cross-sections. The flow-driven effect of the shoulder features of the bobbin-tool design (inscribed grooves) was replicated by the 3D-printed tools and the analogue modelling of the weld samples. Similar flow patterns were achieved by dissimilar aluminium-copper weld, validating the accuracy of the analogue plasticine for the flow visualization of the bobbin friction stir welding.
The Effect of Different Extracts of Beetroots as Antioxidant and Anti-Anaemia On Phenylhydrazine-Induced Rats
Authors: Hikmawanti Ni Putu Ermi , Dwita Lusi Putri , Wisnunanda Dimas W. , Farista Fanny 
Published: April 2021

Abstract

Aim
evaluate antioxidant and anti-anaemia activity of dichloromethane, hydroethanolic, and alkaloids-free hydroethanolic extracts of beetroot (Beta vulgaris (L.) subsp. vulgaris) on phenylhydrazine-induced rats.

Methods
Male rats were divided into five groups: normal control group, negative control group, dichloromethane extract group, hydroethanolic extract group, and alkaloids-free hydroethanolic extract group. All groups were induced with phenylhydrazine (30 mg.Kg−1 BW) for three days, except for the normal control group. After induction, each treatment group received each extract (200 mg.Kg−1 BW) for 21 days. The haematology parameters (haemoglobin levels, the number of erythrocytes, and haematocrit levels) were measured using Haematology Analyzer, and the antioxidant activity was measured through MDA level parameters in rats. Data were analysed using one-way ANOVA and then continued with the Tukey test.

Results
The results showed that the hydroethanolic extract of beetroot increased the percentage of erythrocytes (33.5%), haemoglobin (25%), and haematocrit (24.4%) to the negative control group, which was comparable to the normal control group (p > 0.05). In addition, the best antioxidant activity was shown in the hydroethanolic extract of beetroot, which is comparable to the normal group (p > 0.05).

Conclusion
The beetroot hydroethanolic crude extract could be potentially produced in a natural pharmaceutical product as a beneficial resource within anti-anaemia and antioxidant activities.
Saccharomyces cerevisiae Enhanced the Growth, Immune and Antioxidative Responses of European Seabass (Dicentrarchus labrax)
Authors: Dawood Mahmoud A.O. , Abd El-Kader Marwa F. , Farid Mona A. , Abd-Elghany Mohamed F. , Alkafafy Mohamed , Van Doan Hien 
Published: April 2021

Abstract
The concept of probiotics is widely applied in the field of aquaculture for their beneficial and friendly influences. In this sense, the role of Saccharomyces cerevisiae on the growth, immune and antioxidative responses of European seabass was tested in this study. Fish were distributed in 3 groups (triplicates) with ten fish in each replicate and fed 0, 1, and 2 g/kg of S. cerevisiae (15×109 CFU/g) for 90 days. Fish fed S. cerevisiae showed higher final body, weight gain, and specific growth rate as well as lower FCR than fish fed the basal diet (P<0.05). The RBCs, WBCs, Hb, and PCV values were increased in fish fed dietary S. cerevisiae when compared to the control (P<0.05). The blood total protein, albumin, and globulin were higher in fish fed S. cerevisiae than the control (P<0.05). Fish fed dietary S. cerevisiae had enhanced phagocytic index, phagocytic, and lysozyme activity comparing the control. In a similar sense, the antioxidative enzymes (SOD, GPx, and CAT) were higher in fish fed S. cerevisiae than the control (P<0.05). However, the level of MDA was lowered (P<0.05) by S. cerevisiae in European seabass. Gene expression of IL-8, IL-1β, GH, and IGF-1 was upregulated and HSP70 was downregulated by S. cerevisiae (P<0.05). It can be concluded that European seabass fed S. cerevisiae at 1–2 g/kg (15×109 CFU/g) diet had markedly enhanced growth, haemato-biochemical, and immune performances.
In vivo and in vitro hepatoprotective effect of three endemic plants against carbon tetrachloride-induced liver damage in rats
Authors: Boubellouta Houria , Touhami Fatima Khelifi , Mahdi Djahida 
Published: April 2021

Abstract
The aim of the study was to investigate in vitro potential antioxidant and hepatoprotective effects of methanolic extracts from the aerial parts of Matricaria pubescens, Centaurea incana, and Santolina africana, against carbon tetrachloride induced liver damage in rats. The different aerial parts of three methanolic extracts, exhibited antioxidant activity in the DPPH free radical (IC50, 0.032, 0.087 and 0.044 mg/ml) respectively. The reducing ability of the extracts showed a dose-dependent trend increasing with increase in the concentrations of the extracts.The antioxidant activity of samples at the concentration of 2 mg/ml was reflected in their ability to inhibit the bleaching of β-carotene. Treated groups with extracts of studied three plants showed decrease in plasma levels of AST, ALT, ALP, and an increase in plasma catalase levels activities, while hepatic MDA decreased and catalase increased as compared to carbon tetrachloride treated groups. In consistency with in vivo and in vitro results, extracts of all three plants ameliorated the carbon tetrachloride extensive damage to liver tissue of treated animals, but, Santolina africana extract was the most efficient.
High production of L-glutamic acid from date juice extracts by Corynebacterium glutamicum using fed-batch cultures: pulsed and continuous feeding modes
Authors: Abdenacer Mouffok , Aicha Nancib , Joseph Boudrant , Nabil Nancib 
Published: April 2021

Abstract
In the present work, L-glutamic acid production by Corynebacterium glutamicum fermentation on date juice extracts applying two fed-batch feeding modes, pulsed and continuous, were investigated. According to the obtained results, the continuous feeding fed-batch mode was found to be the most efficient process. Moreover the continuous feeding rate mode with a feeding medium containing date juice sugars enriched with ammonium sulfate was found even more favorable as it enhances the L-glutamic acid production by approximately 2.35 fold more than the batch culture and by about 1.17 fold more than the pulsed feeding. In this respect, comparing the traditional batch culture to the continuously fed culture with a medium containing date juice sugars with ammonium sulfate showed increases of 135.47% in L-glutamic acid production, 104% in productivity, 39.09% in biomass, and 47.69% in the yield respectively allowing us to reach a final L-glutamic acid concentration of about 138 g/L, the highest ever published.