Superantigenic TCR Vbeta 21.3 signature in Multisystem Inflammatory Syndrome in Children
Authors: Moreews Marion , Le Gouge Kenz , Bellomo Alicia , Malcus Christophe , Pescarmona Rémi , Khaldi-Plassart Samira , Djebali Sophia , Mathieu Anne-Laure , Perret Magali , Villard Marine , Chopin Emilie , Rouvet Isabelle , Vandenesh Francois , Dupieux Céline , Pouyau Robin , Teyssedre Sonia , Guerder Margaux , Louazon Tiphaine , Anne-Moulin-Zinsch  , Duperril Marie , Patural Hugues , Giovannini-Chami Lisa , Portefaix Aurélie , Kassai Behrouz , Venet Fabienne , Monneret Guillaume , Lombard Christine , Flodrops Hugues , Bastard Paul , Zhang Shen-Ying , Dubois Valérie , Thaunat Olivier , Richard Jean-Christophe , Mezidi Mehdi , Abel Laurent , Casanova Jean Laurent , Marvel Jacqueline , Trouillet-Assant Sophie , Klatzmann David , Walzer Thierry , Dreux Marlène , Mariotti-Ferrandiz Encarnita , Javouhey Etienne , Belot Alexandre 
Published: February 2021

AbstractObjectivesMultiple Inflammatory Syndrome in Children (MIS-C) is the most severe pediatric form of COVID-19 and occurs in previously healthy children. MIS-C combines features of Kawasaki disease and Toxic Shock Syndrome (TSS).MethodsChildren with suspected MIS-C were included within the first week of diagnosis and a large scale immunoassay was performed to determein the immunologic signature of these patients.ResultsWe characterized the immunological profile of 27 MIS-C cases in comparison with 4 KD and 4 TSS cases. Similarly to TSS, an increase of serum inflammatory cytokines (IL-6, TNF-a, CD25s) was observed in MIS-C contrasting with low expression of HLA-DR monocytes, a feature often associated with immune paralysis. Expansions of T cells expressing the Vβ21.3 T cell receptor β chain variable region were detected in both CD4 and CD8 subsets in almost 50% of patients and Vβ21.3-positive T cells expressed high level of HLA-DR highlighting their specific activation. TCR sequencing uncovered the polyclonal nature of the Vβ 21.3+ population. SARS-CoV2 antigene-specific production of interferon gamma in T cells was not increased in MIS-C T cells compared to COVID-19 patients suggesting the antigen-specific immune response in MIS-C patients is not pivotal to the manifestation.ConclusionsOur findings argue in favor of a strong activation of the immune system related to a superantigenic immune response in MIS-C with a specific polyclonal Vβ21.3 T cell expansion.Key messagesWhat is already known about this subject ?MIS-C occurs 3-5 weeks after acute SARS-CoV2 infection and overlap features of Toxic Shock syndrome and Kawasaki disease.MIS-C appears different in term of cytokine and autoantibodies generation from KD with subtle signs of T cells activationWhat does this study add?This study demonstrates that Vβ21.3+ CD4 and CD8 T cells are highly increased in about 50% of MIS-C and distinctive of the Vβ2+ expansion observed in toxic shock syndrome in This reflects a specific T cell activation and cytokine release syndrome similar to toxic shock syndromeHow mich this impact on clinical practice or future developments?Vβ21.3+ signature can be available on a short term basis by flowcytometry and represents a signature of the MIS-C.As for TSS, immunomodulating therapies may revert the superantigenic activation and resolve this life threatening pediatric condition.