bluuebunny/crossref_metadata_2025_split huggingface dataset:
Food intake, masticatory function, tooth mobility, loss of posterior support, and diminished quality of life are associated with more advanced periodontitis stage diagnosis
Authors: Uy Samantha N. M. R. , Deng Ke , Fok Chris Tat Cheun , Fok Melissa R. , Pelekos George , Tonetti Maurizio S. 
Published: December 2021

AbstractAimTo describe periodontal and functional characteristics of subjects diagnosed with different stages of periodontitis and to associate measures of masticatory function and quality of life with periodontitis stage.Materials and MethodsThis was a cross‐sectional study on a convenience sample of 214 subjects with periodontitis seeking oral care in a hospital setting. They received a full‐mouth intra‐oral examination including dental and periodontal charting by a single calibrated examiner who also established the periodontitis stage diagnosis. Subjects were assessed using the OHIP‐14, a validated masticatory dysfunction questionnaire, and a quantitative test based on the ability to mix a dual colour chewing gum. Mixing was quantified based on the variance of hue (VOH) with a colorimetric software. Descriptive, univariate, and multivariate analyses were performed.ResultsSubjects with stage IV periodontitis reported greater impairment of oral‐health‐related quality of life, reduced food intake or altered food type intake attributed to difficulties in chewing, objective measures of masticatory dysfunction, tooth loss, as well as more advanced periodontal breakdown compared with subjects with stages I–III of the disease. Quantitative assessment of masticatory function was associated, in a multivariate analysis, with (i) loss of functional tooth units in the premolar/molar region, presence of hypermobile teeth, and severity of periodontal attachment loss, and (ii) age, body mass index, and periodontitis stage IV and grade C diagnosis.ConclusionsSubjects with stage IV periodontitis are characterized by a specific set of signs and symptoms of advanced periodontal breakdown and functional impairment, which impact on the quality of life and masticatory function/food intake choices. Stage IV periodontitis captures a clinical entity with distinct features and treatment needs.This study is registered in ClinicalTrials.gov (NCT03928080).
Long non‐coding ribonucleic acid ATP2B1‐AS1 modulates endothelial permeability through regulating the miR‐4729–IQGAP2 axis in diabetic retinopathy
Authors: Ren Zengjin , Wang Xue 
Published: December 2021

ABSTRACTAims/IntroductionMounting evidence shows that long non‐coding RNAs (lncRNAs) are important to modulate the biological process of diabetic retinopathy (DR). We aimed to investigate the role of lncRNAs in DR and elucidate the exact mechanism.Materials and MethodsReal‐time quantitative polymerase chain reaction was carried out to distinguish the lncRNA ATPase plasma membrane Ca2+ transporting 1 antisense RNA 1 (ATP2B1‐AS1) expression in DR patients and HG‐treated human retinal endothelial cells (HRECs). Dual‐luciferase reporter system was used to verify that ATP2B1‐AS1 could act as a microRNA (miR)‐4729 sponge, and miR‐4729 could bind to 3′UTR of IQ motif‐containing GTPase‐activating protein 2 (IQGAP2). Cell proliferation assay, wound healing migration assay, transwell assay, tube formation assay and immunofluorescence were used to investigate cell proliferation, migration and angiogenesis in HRECs.ResultsThe present results showed that ATP2B1‐AS1 was downregulated in DR patients and high‐glucose‐induced HRECs. In gain‐ and loss‐of‐function assays, ATP2B1‐AS1 overexpression could significantly reduce cell proliferation, migration, angiogenesis and permeability induced by high glucose in vitro. Additionally, we carried out dual‐luciferase reporter experiments to determine that ATP2B1‐AS1 could act as a miR‐4729 sponge. ATP2B1‐AS1 overexpression could rescue miR‐4729 mimics and short hairpin RNA‐IQGAP2 induced cell proliferation, migration and angiogenesis in HRECs.ConclusionsThe present study showed that ATP2B1‐AS1 acted as a miR‐4729 sponge to regulate IQGAP2 reducing high‐glucose‐induced endothelial dysfunction in DR.
A mixed‐methods evaluation of home‐based primary care in dementia within an integrated system
Authors: Nguyen Huong Q. , Vallejo Jessica D. , Macias Mayra , Shiffman Maricela Garcia , Rosen Romina , Mowry Velda , Omotunde Omotayo , Hong Benjamin , Liu In‐Lu Amy , Borson Soo 
Published: December 2021

AbstractBackgroundNo prior studies have examined the effects of home‐based primary care (HBPC) in persons living with dementia (PLWD), within an ecosystem of serious illness care in an integrated healthcare system. Our objectives were to compare the characteristics of PLWD receiving HBPC and their hospital utilization and end‐of‐life care, with those of a matched comparison group, and to understand the experiences of family caregivers of PLWD receiving HBPC.MethodsThis mixed‐methods study used a retrospective observational cohort design with PLWD receiving HBPC (n = 287) from 2015 to 2020 and a strata‐matched comparison group (n = 861), and qualitative phone interviews with 16 HBPC family caregivers in 2020. Inverse probability of treatment weighting propensity score‐adjusted models were used to compare time‐to‐first hospital‐based utilization and, for decedents, home palliative and hospice care and place of death. Care experience was captured through caregiver interviews.ResultsPatients receiving HBPC had a similar risk of hospital utilization [adjusted hazard ratio, 1.06 (95% CI: 0.89–1.26), p = 0.51] as a matched non‐HBPC comparison group after a median follow‐up of 199 days. However, HBPC decedents (n = 159) were more likely to receive home palliative care or hospice [rate ratio, RR: 1.23 (95% CI: 1.07–1.42), p < 0.01] and to die at home [RR: 1.66 (95% CI: 1.35–2.05), p < 0.001] than were non‐HBPC decedents (n = 423). Caregivers reported that HBPC provided coordinated, continuous, and convenient care that was aligned with families' priorities and goals; however, some expressed unmet needs, especially for help paying for personal care and medical supplies/equipment, and a desire for clearer communication about program operations and more quality oversight for contract services.ConclusionsAlthough HBPC for PLWD was associated with a similar risk of hospital utilization compared to a matched non‐HBPC comparison group, HBPC resulted in more patient‐centered end‐of‐life care for decedents. Prospective studies of HBPC that further elicit and address unmet needs are warranted.
The association between dietary factors and body weight and composition in boys with Duchenne muscular dystrophy
Authors: Billich Natassja , Evans Maureen , Truby Helen , Ryan Monique M. , Davidson Zoe E. 
Published: December 2021

AbstractBackgroundDuchenne muscular dystrophy (DMD) is a X‐linked neuromuscular disorder. Boys with DMD have high rates of obesity, although little is known about dietary factors that may contribute to weight gain in this population. The present study aimed to explore the relationship between dietary factors, body mass index (BMI) z‐score, body composition and motor function and to describe dietary intake in boys with DMD.MethodsA cross‐sectional analysis of 3‐day food diaries from ambulant and steroid treated boys with DMD aged 5–13 years was conducted. Correlation analysis explored the relationship between dietary factors, BMI z‐score, fat mass percentage (FM%) and lean mass percentage (LM%).ResultsThe median age was 8.5 years (interquartile range [IQR] = 7.2–10.5 years). Median energy kg–1 day–1 in those within a healthy weight range (n = 11) was 316 kJ kg–1 day–1 (IQR = 276–355 kJ kg–1 day–1) and greater than estimated requirements and, for those above a healthy weight (n = 26), energy intake was 185 kJ kg–1 day–1 (IQR = 143–214 kJ kg–1 day–1) and lower than estimated requirements. Energy kg–1 day–1 was negatively associated with BMI z‐score (r = −0. 650) and FM% (r = −0.817) but positively associated with LM% (r = 0.805; all analyses p = <0.01). Younger age was associated (r = −0.609 p = <0.01) with a higher energy kg–1 day–1. For all participants, vegetable, grains, meat/alternatives and dairy intakes were sub‐optimal.ConclusionsYounger boys with DMD within a healthy weight range are overconsuming energy dense nutrient poor foods. A focus on improving diet quality during early childhood may prove to be a useful strategy for reducing excess weight gain and supporting healthier eating habits in this vulnerable clinical population.
Early recognition method for patients with schizophrenia or bipolar disorder in community mental health care: Illness insight, self‐management and control
Authors: Johansen Kirsten Kjær , Marcussen Jette , Hansen Jens Peter , Hounsgaard Lise , Fluttert Frans 
Published: December 2021

AbstractAims and objectiveTo investigate how participating in the early recognition method treatment strategy affect illness insight and management, in patients with schizophrenia or bipolar disorder in community mental healthcare.BackgroundThe current practice in mental healthcare focus on shared decision‐making and self‐managing capacity, but poor insight is a predictor of poor adherence and dropout. Engagement in illness management and recovery predict the treatment response.DesignSemi‐structured interviews with a phenomenological‐hermeneutic approach.MethodsWe conducted 36 semi‐structured interviews with 26 patients. The interviews were conducted before and after participating in the intervention using the early recognition method strategy. The analysis was based on Ricoeur's theory of interpretation: Naive reading, structural analysis, interpretation and discussion. The COREQ checklist was used as reporting guideline.ResultsThe experience of participating in treatment as usual and early recognition method revealed two main themes. The first theme ‘patient care’ describes how dialogue and collaboration increase awareness of the illness and how to gain control. The second theme ‘insight and experience’ describes how illness affects personality and self‐image, and how insight entails control and self‐confidence.ConclusionManaging life with severe mental illness is complex and challenging. However, the experience of guidance, support and collaboration between patient and nurse are essential to improve these circumstances.Relevance to clinical practiceA systematic approach to the patient' symptoms, as in the early recognition method strategy, enhances knowledge of the individual patient' symptoms, both for nurse and patient. A knowledge that is significant for meeting individual treatment needs. Therefore, applying this strategy is likely to enhance collaboration and improve treatment outcome.
Image diagnosis models for the oral assessment of older people using convolutional neural networks: A retrospective observational study
Authors: Muramatsu Misato , Muramatsu Masumi , Takahashi Naoto , Hagiwara Atsuko , Hagiwara Jyun , Takamatsu Yuichiro , Morooka Ryo , Ochi Morio , Kaitani Toshiko 
Published: December 2021

AbstractAimsThe purpose of this study was to construct a model for oral assessment using deep learning image recognition technology and to verify its accuracy.BackgroundThe effects of oral care on older people are significant, and the Oral Assessment Guide has been used internationally as an effective oral assessment tool in clinical practice. However, additional training, education, development of user manuals and continuous support from a dental hygienist are needed to improve the inter‐rater reliability of the Oral Assessment Guide.DesignA retrospective observational study.MethodsA total of 3,201 oral images of 114 older people aged >65 years were collected from five dental‐related facilities. These images were divided into six categories (lips, tongue, saliva, mucosa, gingiva, and teeth or dentures) that were evaluated by images, out of the total eight items that comprise components of the Oral Assessment Guide. Each item was classified into a rating of 1, 2 or 3. A convolutional neural network, which is a deep learning method used for image recognition, was used to construct the image recognition model. The study methods comply with the STROBE checklist.ResultsWe constructed models with a classification accuracy of 98.8% for lips, 94.3% for tongue, 92.8% for saliva, 78.6% for mucous membranes, 93.0% for gingiva and 93.6% for teeth or dentures.ConclusionsHighly accurate diagnostic imaging models using convolutional neural networks were constructed for six items of the Oral Assessment Guide and validated. In particular, for the five items of lips, tongue, saliva, gingiva, and teeth or dentures, models with a high accuracy of over 90% were obtained.Relevance to Clinical PracticeThe model built in this study has the potential to contribute to obtain reproducibility and reliability of the ratings, to shorten the time for assessment, to collaborate with dental professionals and to be used as an educational tool.
Assessment of the inter‐rater agreement of corneal cytology and culture findings in canine ulcerative keratitis
Authors: Hamzianpour N. , Adams V. J. , Grundon R. A. , Linn‐Pearl R. , Scurrell E. , Rozmanec M. , Civello A. , Goss R. , Watkins C. , Kearns H. , Heinrich C. 
Published: December 2021

ObjectivesTo assess the inter‐rater agreement of corneal cytology findings in canine ulcerative keratitis by veterinary surgeons of different training levels and the agreement of corneal cytology with culture.Materials and MethodsDogs with progressive ulcerative keratitis were prospectively recruited for corneal cytology and culture. Corneal cytology slides were reviewed by veterinary surgeons of different training levels (three general practitioners, three ophthalmologists and three pathologists). The inter‐rater agreement of cytology findings and agreement of cytology with culture was assessed using the kappa measure of agreement.ResultsThe study included 145 corneal cytology samples from 143 dogs (145 eyes) with progressive ulcerative keratitis. Positive cultures were obtained from 81 of 145 (56%) eyes. The most commonly isolated pathogens wereStreptococcus canis,Pseudomonas aeruginosaandStaphylococcus pseudintermedius. The results demonstrated increased inter‐rater agreement of corneal cytology and increased agreement with culture with increased ocular pathology expertise (pathologists > ophthalmologists > general practitioners).Clinical SignificanceThis study provides important information about the diagnostic value of corneal cytology in canine ulcerative keratitis and the most common pathogens involved in such cases in the UK. Based on the results of this study, cytology findings should be interpreted in conjunction with the expertise of the observer. For maximal pathogen identification, both cytology and culture should be considered.
The legislative framework of donor human milk and human milk banking in Europe
Authors: Klotz Daniel , Wesołowska Aleksandra , Bertino Enrico , Moro Guido E. , Picaud Jean‐Charles , Gayà Antoni , Weaver Gillian 
Published: December 2021

AbstractData about the regulatory approaches to donor human milk (DHM) in European countries are lacking. The aim of this study is to describe the various regulations of DHM within European countries, to assess its legislative context and its impact in relation to donor milk banking. We performed a cross‐sectional survey using a semistructured online questionnaire addressing 29 national European milk‐banking representatives from June 2020 to February 2021. Representatives of 26 national DHM services participated in this study. The legal classification and regulatory status of DHM were defined in 9 out of 26 areas of jurisdiction (35%) as either food product (n = 6), product of human origin according to a blood, tissue, cell regulation (n = 2), or medicinal product (n = 1). In the remainder, DHM remains unclassified. Most legislations did not provide a comprehensive framework concerning DHM and costs to cover milk bank operations were rarely reimbursed. In general, the lack of national legislative governance and the actual legislative regulations in place do not support the use of DHM in European countries. National medical guidelines for the use of DHM have been issued in only 11 countries. The current number and distribution of milk banks (n = 239) within participating countries may not provide an equitable access to DHM for eligible infants. These findings could guide stakeholders aiming to establish a regulatory framework for DHM.
DNA metabarcoding reveals the importance of gelatinous zooplankton in the diet ofPandalus borealis, a keystone species in the Arctic
Authors: Urban Paulina , Præbel Kim , Bhat Shripathi , Dierking Jan , Wangensteen Owen S. 
Published: December 2021

AbstractInformation about the dietary composition of a species is crucial to understanding their position and role in the food web. Increasingly, molecular approaches such as DNA metabarcoding are used in studying trophic relationships, not least because they may alleviate problems such as low taxonomic resolution or underestimation of digestible taxa in the diet. Here, we used DNA metabarcoding with universal primers for cytochromecoxidase I (COI) to study the diet composition of the northern shrimp (Pandalus borealis), an Arctic keystone species with large socio‐economic importance. Across locations, jellyfish and chaetognaths were the most important components in the diet ofP. borealis, jointly accounting for 40%–60% of the total read abundance. This dietary importance of gelatinous zooplankton contrasts sharply with published results based on stomach content analysis. At the same time, diet composition differed between fjord and shelf locations, pointing to different food webs supportingP. borealisin these two systems. Our study underlines the potential of molecular approaches to provide new insights into the diet of marine invertebrates that are difficult to obtain with traditional methods, and calls for a revision of the role of gelatinous zooplankton in the diet of the key Arctic speciesP. borealis, and in extension, Arctic food webs.
Seismic adaptive control of building structures with simultaneous sensor and damper faults based on dynamic neural network
Authors: Naderpoor Pegah , Taghikhany Touraj 
Published: December 2021

AbstractThe use of seismic control systems in building structures requires simultaneous attention to uncertainties in model parameters, measurement noises, faults on sensors and dampers, and earthquake input. The present study suggests a procedure to resolve the complications of these issues in semi‐active control of building structures. To this end, the effects of faults on sensors and dampers are decoupled by using transfer matrices. The sensor fault and the structure's state are estimated by a dynamic neural network (DNN)‐based observer. Later, parametric uncertainty in the structure's model and fault on dampers are atoned by an adaptive controller that guarantees the stability of the system during an earthquake. In this controller, DNN is used to concurrently identify and compensate dampers' faults. The effectiveness of the proposed method was confirmed by numerical simulation of a three‐story structure with magneto‐rheological dampers.
Compulsory notification of paracoccidioidomycosis: A 14‐year retrospective study of the disease in the state of Paraná, Brazil
Authors: de Suguiura Igor Massahiro Souza , Ono Mario Augusto 
Published: December 2021

AbstractBackgroundParacoccidioidomycosis, caused by fungi of the Paracoccidioides genus, is one of the most important endemic mycoses in Brazil. The disease is not of mandatory reporting in the country; however, some Brazilian states, such as Paraná, have included it on their local lists of public health‐reportable diseases.ObjectiveDescribe the epidemiology of the positive paracoccidioidomycosis cases in the state of Paraná based on analysis of reporting forms and mortality data.Subjects and methodsData of positive cases of state residents 2007–2020 were obtained from public health databases and frequency, incidence, geographic distribution, mortality and trends were analysed. Mortality of the disease was also compared to other mycoses such as cryptococcosis, aspergillosis, histoplasmosis, candidiasis and sporotrichosis.Results670 patients were positive for the disease. The cumulative and average annual incidence was, respectively, 6.4 and 0.46 cases/100,000 inhabitants. The new cases of paracoccidioidomycosis were reported mainly by specialised health units, including tertiary centres, and 285 days was the mean from the beginning of the symptoms until the diagnosis. The western region showed the highest incidence and mortality by the disease over the other state mesoregions. During the period, a decreasing trend was observed in the confirmed cases and stability in the mortality rate with an average annual mortality of 1.17 per million/inhabitants in the state; however, paracoccidioidomycosis had the highest mortality when compared to other mycoses.ConclusionsParacoccidioidomycosis is an important endemic mycosis in Paraná and this study provides an epidemiological baseline for future modifications of paracoccidioidomycosis surveillance.
A missense mutation in WRKY32 converts its function from a positive regulator to a repressor of photomorphogenesis
Authors: Zhou Hua , Zhu Wei , Wang Xuncheng , Bian Yeting , Jiang Yan , Li Jian , Wang Lixia , Yin Ping , Deng Xing Wang , Xu Dongqing 
Published: December 2021

Summary

CONSTITUTIVELY PHOTOMORPHOGENIC 1 (COP1) mediates various cellular and physiological processes in plants by targeting a large number of substrates for ubiquitination and degradation.

In this study, we reveal that a substitution of Pro for Leu at amino acid position 409 in WRKY32 largely suppresses the short hypocotyls and expanded cotyledon phenotypes of cop1‐6. WRKY32P409L promotes hypocotyl growth and inhibits the opening of cotyledons in Arabidopsis.

Loss of WRKY32 function mutant seedlings display elongated hypocotyls, whereas overexpression of WRKY32 leads to shortened hypocotyls. WRKY32 directly associates with the promoter regions of HY5 to activate its transcription. COP1 interacts with and targets WRKY32 for ubiquitination and degradation in darkness. WRKY32P409L exhibits enhanced DNA binding ability and affects the expression of more genes compared with WRKY32 in Arabidopsis.

Our results not only reveal the basic role for WRKY32 in promoting photomorphogenesis, but also provide insights into manipulating plant growth by engineering key components of light signaling.