bluuebunny/crossref_metadata_2025_split huggingface dataset:
Kaolinite-armoured polyurea microcapsules fabricated on Pickering emulsion: controllable encapsulation and release performance of a lipophilic compound
Authors: Li Cunjun , Wang Minghao , Liu Zhaoliang , Xu Yanqi , Zhou Chunhui , Wang Linjiang 
Published: April 2021

AbstractMicrocapsules are successfully used in various applications such as self-healing, drug delivery and military camouflage. The shells of the microcapsules based on the traditional surfactant-stabilized emulsion template method are often single organic materials. The surfactants generally have insufficient stability against demulsification during preparation of the microcapsules. In the present study, kaolinite was used as an emulsifier for stabilizing Pickering emulsions and subsequently as an enhancer for forming microcapsules. Kaolinite-armoured polyurea microcapsules were fabricated based on the interfacial polymerization of isophorone diisocyanate at the oil–water interfaces of kaolinite-stabilized Pickering emulsions. The prepared microcapsules with core–shell structure were spherical and exhibited good dispersibility in anhydrous ethanol. The shell thickness (~0.5–1.0 μm) and diameter (~20.0–160.0 μm) of kaolinite-armoured polyurea microcapsules may be tailored by varying the dosages of isophorone diisocyanate and kaolinite and the emulsifying speed of the high-shear homogenizer. Hence, the encapsulation and release performance of microcapsules may be controlled. The kaolinite particles were embedded and armoured in a polyurea matrix. The formed kaolinite-embedded and -armoured polyurea structures might prolong the release of the encapsulated lipophilic Sudan Red (III) from 20 to 45 h. The microcapsules have controllable encapsulation and release characteristics for lipophilic compounds and are cost effective, making them suitable pesticides.
Cancer immune control dynamics: a clinical data driven model of systemic immunity in patients with metastatic melanoma
Authors: Frisch Harold P. , Sprau Allan , McElroy Virginia F. , Turner James D. , Becher Laura R. E. , Nevala Wendy K. , Leontovich Alexey A. , Markovic Svetomir N. 
Published: April 2021

AbstractBackgroundRecent clinical advances in cancer immuno-therapeutics underscore the need for improved understanding of the complex relationship between cancer and the multiple, multi-functional, inter-dependent, cellular and humoral mediators/regulators of the human immune system. This interdisciplinary effort exploits engineering analysis methods utilized to investigate anomalous physical system behaviors to explore immune system behaviors. Cancer Immune Control Dynamics (CICD), a systems analysis approach, attempts to identify differences between systemic immune homeostasis of 27 healthy volunteers versus 14 patients with metastatic malignant melanoma based on daily serial measurements of conventional peripheral blood biomarkers (15 cell subsets, 35 cytokines). The modeling strategy applies engineering control theory to analyze an individual’s immune system based on the biomarkers’ dynamic non-linear oscillatory behaviors. The reverse engineering analysis uses a Singular Value Decomposition (SVD) algorithm to solve the inverse problem and identify a solution profile of the active biomarker relationships. Herein, 28,605 biologically possible biomarker interactions are modeled by a set of matrix equations creating a system interaction model. CICD quantifies the model with a participant’s biomarker data then computationally solves it to measure each relationship’s activity allowing a visualization of the individual’s current state of immunity.ResultsCICD results provide initial evidence that this model-based analysis is consistent with identified roles of biomarkers in systemic immunity of cancer patients versus that of healthy volunteers. The mathematical computations alone identified a plausible network of immune cells, including T cells, natural killer (NK) cells, monocytes, and dendritic cells (DC) with cytokines MCP-1 [CXCL2], IP-10 [CXCL10], and IL-8 that play a role in sustaining the state of immunity in advanced cancer.ConclusionsWith CICD modeling capabilities, the complexity of the immune system is mathematically quantified through thousands of possible interactions between multiple biomarkers. Therefore, the overall state of an individual’s immune system regardless of clinical status, is modeled as reflected in their blood samples. It is anticipated that CICD-based capabilities will provide tools to specifically address cancer and treatment modulated (immune checkpoint inhibitors) parameters of human immunity, revealing clinically relevant biological interactions.
Study of vitamin D receptor gene polymorphisms in a cohort of myocardial infarction patients with coronary artery disease
Authors: Raljević Damir , Peršić Viktor , Markova-Car Elitza , Cindrić Leon , Miškulin Rajko , Žuvić Marta , Kraljević Pavelić Sandra 
Published: April 2021

Abstract
Background
Vitamin D deficiency is associated with cardiovascular diseases, including coronary artery diseases (CAD). As vitamin D manifests its biological function through its vitamin D receptor (VDR), VDR gene polymorphisms potentially affect VDR functionality and vitamin D activity. Therefore, the objective of this study was to analyze three well-studied VDR gene polymorphisms—Fok1 (rs2228570), BsmI (rs1544410) and Taq1 (rs731236)—in a cohort of CAD patients after acute myocardial infarction.

Methods
In the presented cross-sectional study, 155 participants with CAD after acute myocardial infarction and 104 participants in a control group without CAD were enrolled. The participants in both groups were Caucasians of European origin. The genotyping of VDR polymorphisms rs2228570, rs1544410 and rs731236 was assessed by RT-PCR.

Results
The results show an association between the T/T genotype of the BsmI (rs1544410) and the G/G genotype of the Taq1 (rs731236) VDR polymorphism and CAD patients after acute myocardial infarction. There was no association between the Fok1 (rs2228570) VDR polymorphism and CAD patients after acute myocardial infarction.

Conclusion
The presented results suggest a potential association of the BsmI (rs1544410) and Taq1 (rs731236) VDR polymorphisms with CAD patients after myocardial infarction.
Effects of person-environment fit of gender-role orientation on burnout, engagement and hair steroids as stress biomarkers among women
Authors: Wacker Eva , Fischer Axel , Schorlemmer Julia 
Published: April 2021

Abstract
Background
Analysis on gender related differences in occupational stress and burnout levels usually reveal higher occupational stress and burnout levels for women compared to men, especially in male-dominated working environments. In opposition to group differentiation, more specific gender-related dimensions feminity and masculinity were used in the study to describe individual and work environment characteristics and analyze their effects.

Methods
In a cross-sectional design, survey results were linked to steroid levels in hair samples. Data was collected in a German medical services company with 146 employed women age 22–66 years (M = 40.48, SD = 10.38), 58 of them provided hair samples for steroid detection. Feminity and masculinity were measured by Gender Role Orientation Scale GTS+. Two Person-Environment fit scores in feminity and masculinity were calculated by subtracting individual from environment values. Both fit scores were proved as predictors in hierarchical linear regression models predicting burnout and work engagement as well as hair steroids cortisol, cortisone, DHEA, testosterone and progesterone detected by Liquid Chromatography-Mass Spectrometry (LC-MS/MS) as stress biomarkers. Bivariate correlations as well as moderator and mediator analysis were implemented.

Results
After considering age, role clarity, and work organization, Person-Environment fit in feminity still added significant variance explanation (β = .23, ∆ R2 = .05, p = .003) for burnout. Person-Environment fit in feminity also explained poor variance in work engagement (β = −.29, R2 = .09, p < .001). Person-Environment fit in masculinity added considerable variance explanation (β = .34, ∆ R2 = .12, p = 0.018) to cortisol levels after including quantitative demands to the model.

Conclusions
Person-Environment fit in feminity might be inspected as a predictor for burnout and work engagement. Person-Environment fit in masculinity can be taken into consideration as a predictor for hair cortisol as stress biomarker. Feminity and masculinity can be used as personality traits as well as characteristics of work environment, thus providing a particular gender-role related method of differentiation within gender groups. Also, specific methods could be derived for stress and burnout prevention and promotion of work engagement. Representative population studies with bigger samples and longitudinal surveys are needed to better explore the benefits and limitations of this approach.
Identification of X-chromosomal genes that drive sex differences in embryonic stem cells through a hierarchical CRISPR screening approach
Authors: Genolet Oriana , Monaco Anna A. , Dunkel Ilona , Boettcher Michael , Schulz Edda G. 
Published: April 2021

AbstractBackgroundX-chromosomal genes contribute to sex differences, in particular during early development, when both X chromosomes are active in females. Double X-dosage shifts female pluripotent cells towards the naive stem cell state by increasing pluripotency factor expression, inhibiting the differentiation-promoting MAP kinase (MAPK) signaling pathway, and delaying differentiation.ResultsTo identify the genetic basis of these sex differences, we use a two-step CRISPR screening approach to comprehensively identify X-linked genes that cause the female pluripotency phenotype in murine embryonic stem cells. A primary chromosome-wide CRISPR knockout screen and three secondary screens assaying for different aspects of the female pluripotency phenotype allow us to uncover multiple genes that act in concert and to disentangle their relative roles. Among them, we identify Dusp9 and Klhl13 as two central players. While Dusp9 mainly affects MAPK pathway intermediates, Klhl13 promotes pluripotency factor expression and delays differentiation, with both factors jointly repressing MAPK target gene expression.ConclusionsHere, we elucidate the mechanisms that drive sex-induced differences in pluripotent cells and our approach serves as a blueprint to discover the genetic basis of the phenotypic consequences of other chromosomal effects.
Maternal drinking and smoking. Can it explain the exceptional academic performance of LBOTE children? A preliminary analysis
Authors: Gibson Louisa , Porter Melanie 
Published: April 2021

Abstract
Objective
Although children from language backgrounds other than English (LBOTE) may be disadvantaged in English-reliant exams, they outperform children from an English language background (ELB) on many Australian National Assessment Program–Literacy and Numeracy (NAPLAN) assessments. Maternal alcohol and tobacco use during pregnancy and/or breastfeeding have been associated with poorer cognitive and academic performance. Using data from the Growing Up in Australia Study, this paper aimed to identify demographic, lifestyle, and prenatal and perinatal risk differences related to maternal tobacco and alcohol use between LBOTE and ELB groups, as a first step in trying to understand the academic performance differences.

Results
Only data from breastfed babies was included in the current analyses. Although LBOTE children were disadvantaged in several demographic areas, their NAPLAN performance was the same or superior to ELB children across all Grade 3 and 5 NAPLAN assessments. The LBOTE group were, however, breastfed for longer, and their mothers smoked fewer cigarettes and drank less alcohol on fewer occasions throughout their pregnancy. The LBOTE mothers also had lower or less risky patterns of alcohol consumption while breastfeeding. The longer breastfeeding duration of LBOTE children combined with lower maternal use of alcohol and cigarettes during pregnancy and/or breastfeeding may partially contribute to their exceptional NAPLAN performance.
Postoperative malignant hyperthermia confirmed by calcium-induced calcium release rate after breast cancer surgery, in which prompt recognition and immediate dantrolene administration were life-saving: a case report
Authors: Miyazaki Natsumi , Kobayashi Takayuki , Komiya Takako , Okada Toshio , Ishida Yusuke , Fukui Hidekimi , Ogihara Yukihiko , Uchino Hiroyuki 
Published: April 2021

Abstract
Background
Malignant hyperthermia (MH) is a rare genetic disease characterized by the development of very serious symptoms, and hence prompt and appropriate treatment is required. However, postoperative MH is very rare, representing only 1.9% of cases as reported in the North American Malignant Hyperthermia Registry (NAMHR). We report a rare case of a patient who developed sudden postoperative hyperthermia after mastectomy, which was definitively diagnosed as MH by the calcium-induced calcium release rate (CICR) measurement test.

Case presentation
A 61-year-old Japanese woman with a history of stroke was hospitalized for breast cancer surgery. General anesthesia was introduced by propofol, remifentanil, and rocuronium. After intubation, anesthesia was maintained using propofol and remifentanil, and mastectomy and muscle flap reconstruction surgery was performed and completed without any major problems. After confirming her spontaneous breathing, sugammadex was administered and she was extubated. Thereafter, systemic shivering and masseter spasm appeared, and a rapid increase in body temperature (maximum: 38.9 °C) and end-tidal carbon dioxide (ETCO2) (maximum: 59 mmHg) was noted. We suspected MH and started cooling the body surface of the axilla, cervix, and body trunk, and administered chilled potassium-free fluid and dantrolene. After her body temperature dropped and her shivering improved, dantrolene administration was ended, and finally she was taken to the intensive care unit (ICU). Body cooling was continued within the target range of 36–37 °C in the ICU. No consciousness disorder, hypotension, increased serum potassium level, metabolic acidosis, or cola-colored urine was observed during her ICU stay. Subsequently, her general condition improved and she was discharged on day 12. Muscle biopsy after discharge was performed and provided a definitive diagnosis of MH.

Conclusions
The occurrence of MH can be life-threatening, but its frequency is very low, and genetic testing and muscle biopsy are required to confirm the diagnosis. On retrospective evaluation using the malignant hyperthermia scale, the present case was almost certainly that of a patient with MH. Prompt recognition and immediate treatment with dantrolene administration and body cooling effectively reversed a potentially fatal syndrome. This was hence a valuable case of a patient with postoperative MH that led to a confirmed diagnosis by CICR.
The clinical effects of cerebral near-infrared spectroscopy monitoring (NIRS) versus no monitoring: a protocol for a systematic review with meta-analysis and trial sequential analysis
Authors: Hansen Mathias Lühr , Hyttel-Sørensen Simon , Jakobsen Janus Christian , Gluud Christian , Kooi Elisabeth M. W. , Mintzer Jonathan , de Boode Willem P. , Fumagalli Monica , Alarcon Ana , Alderliesten Thomas , Greisen Gorm 
Published: April 2021

AbstractBackgroundMultiple clinical conditions are associated with cerebral hypoxia/ischaemia and thereby an increased risk of hypoxic-ischaemic brain injury. Cerebral near-infrared spectroscopy monitoring (NIRS) is a tool to monitor brain oxygenation and perfusion, and the clinical uptake of NIRS has expanded over recent years. Specifically, NIRS is used in the neonatal, paediatric, and adult perioperative and intensive care settings. However, the available literature suggests that clinical benefits and harms of cerebral NIRS monitoring are uncertain. As rates of clinically significant hypoxic-ischaemic brain injuries are typically low, it is difficult for randomised clinical trials to capture a sufficiently large number of events to evaluate the clinical effect of cerebral NIRS monitoring, when focusing on specific clinical settings. The aim of this systematic review will be to evaluate the benefits and harms of clinical care with access to cerebral NIRS monitoring versus clinical care without cerebral NIRS monitoring in children and adults across all clinical settings.MethodsWe will conduct a systematic review with meta-analysis and trial sequential analysis. We will only include randomised clinical trials. The primary outcomes are all-cause mortality, moderate or severe persistent cognitive or neurological deficit, and proportion of participants with one or more serious adverse events. We will search CENTRAL, EMBASE, MEDLINE, and the Science Citation Index Expanded from their inception and onwards. Two reviewers will independently screen all citations, full-text articles, and extract data. The risk of bias will be appraised using the Cochrane risk of bias tool version 2.0. If feasible, we will conduct both random-effects meta-analysis and fixed-effect meta-analysis of outcome data. Additional analysis will be conducted to explore the potential sources of heterogeneity (e.g. risk of bias, clinical setting).DiscussionAs we include trials across multiple clinical settings, there is an increased probability of reaching a sufficient information size. However, heterogeneity between the included trials may impair our ability to interpret results to specific clinical settings. In this situation, we may have to depend on subgroup analyses with inherent increased risks of type I and II errors.Systematic review registrationPROSPEROCRD42020202986. This systematic review protocol has been submitted for registration in the International Prospective Register of Systematic Reviews (PROSPERO) (http://www.crd.york.ac.uk/prospero) on the 12th of October 2020 and published on the 12th of November 2020 (registration IDCRD42020202986).
Influence of the link between resources and behavioural factors on the entrepreneurial intentions of electrical installation and maintenance work students
Authors: Ohanu Ifeanyi Benedict , Shodipe Taiwo Olabanji 
Published: April 2021

AbstractWith a theory of planned behaviour (TPB) framework, this study examined how the link between resources and behavioural factors influence the entrepreneurial intentions of electrical installation and maintenance work (EIMW) students. It showed how available resources, which are defined as those controlled by the technical institutions but are accessible by the students, influence their behaviour and consequently their entrepreneurial intention. Different approaches are used in the study to analyse the primary data collected from EIMW students and an empirical analysis was done using structural equation model. The study showed the robustness of TPB model in the face of available resource that attitude, subjective norms and perceived behavioural control are behavioural factors that can be positively influence by several exogenous factors to enhance EIMW students’ entrepreneurial intentions. The results of the study enhance the institutional training of staff and instructors at all management to give career counselling services and be vast in the career opportunities available in the field of study. This skill will motivate EIMW students towards establishing their business venture.
Effects of climate adaptation on technical efficiency of maize production in Northern Ghana
Authors: Adzawla William , Alhassan Hamdiyah 
Published: April 2021

AbstractClimate adaptation is an essential strategy for responding to climate change at local levels and required for sustainable food production to meet the growing food demand. In this light, this study analyzed the effects of climate adaptation strategies on technical efficiency of maize farmers in Northern Ghana. This involved a total of 619 maize farmers that were selected through a multistage sampling procedure. A Cobb-Douglas stochastic frontier was fitted to the data. From the result, the major climate adaptation strategies adopted by the farmers include row planting, changing planting date, mixed farming, refilling, and intercropping. The frontier result shows that while climate adaptation significantly leads to higher maize outputs, only crop rotation and row planting significantly improve technical efficiency of maize farmers. Other factors that significantly influence maize output are farm size, labor, seed, and chemicals. The study concludes that climate adaptation, particularly, crop rotation and row planting, remains essential adaptation strategies for sustainable food production in the region. However, further understanding of mechanisms through which majority of the climate adaptation strategies significantly reduce technical efficiency is required.
Tumor suppressor SMAR1 regulates PKM alternative splicing by HDAC6-mediated deacetylation of PTBP1
Authors: Choksi Arpankumar , Parulekar Apoorva , Pant Richa , Shah Vibhuti Kumar , Nimma Ramakrishna , Firmal Priyanka , Singh Smriti , Kundu Gopal C. , Shukla Sanjeev , Chattopadhyay Samit 
Published: April 2021

Abstract
Background
Highly proliferating cancer cells exhibit the Warburg effect by regulation of PKM alternative splicing and promoting the expression of PKM2. Majority of the alternative splicing events are known to occur in the nuclear matrix where various MARBPs actively participate in the alternative splicing events. SMAR1, being a MARBP and an important tumor suppressor, is known to regulate the splicing of various cancer-associated genes. This study focuses on the regulation of PKM alternative splicing and inhibition of the Warburg effect by SMAR1.

Methods
Immunohistochemistry was performed in breast cancer patient samples to establish the correlation between SMAR1 and PKM isoform expression. Further, expression of PKM isoforms upon modulation in SMAR1 expression in breast cancer cell lines was quantified by qRT-PCR and western blot. The acetylation status of PTBP1 was estimated by immunoprecipitation along with its enrichment on PKM pre-mRNA by CLIP in SMAR1 knockdown conditions. The role of SMAR1 in tumor metabolism and tumorigenesis was explored by in vitro enzymatic assays and functional assays upon SMAR1 knockdown. Besides, in vivo tumor formation by injecting adeno-SMAR1-transduced MDA-MB-231 cells in NOD/SCID mice was performed.

Results
The expression profile of SMAR1 and PKM isoforms in breast cancer patients revealed that SMAR1 has an inverse correlation with PKM2 and a positive correlation with PKM1. Further quantitative PKM isoform expression upon modulation in SMAR1 expression also reflects that SMAR1 promotes the expression of PKM1 over tumorigenic isoform PKM2. SMAR1 deacetylates PTBP1 via recruitment of HDAC6 resulting in reduced enrichment of PTBP1 on PKM pre-mRNA. SMAR1 inhibits the Warburg effect, tumorigenic potential of cancer cells, and in vivo tumor generation in a PKM2-dependent manner.

Conclusions
SMAR1 regulates PKM alternative splicing by causing HDAC6-dependent deacetylation of PTBP1, resulting in reduced enrichment of PTBP1 on PKM pre-mRNA. Additionally, SMAR1 suppresses glucose utilization and lactate production via repression of PKM2 expression. This suggests that tumor suppressor SMAR1 inhibits tumor cell metabolism and tumorigenic properties of cancer cells via regulation of PKM alternative splicing.