bluuebunny/crossref_metadata_2025_split huggingface dataset:
HSP105 expression in cutaneous malignant melanoma: Correlation with clinicopathological characteristics
Authors: Chen Ke-Jun , Li Feng-Zeng , Ye Qian , Jia Meng , Fang Sheng 
Published: October 2021

Background
Heat shock proteins can protect against stress-associated cellular challenges, but they can also protect some tumors from human immune system monitoring. Heat shock protein 105 (HSP105/110) is a high molecular weight protein whose expression has been reported in many cancers, but few studies on its role in cutaneous malignant melanoma have been published. In this study, we analyzed the relationship between HSP105 expression and the clinicopathological characteristics of CMM.

Methods
This retrospective study included 91 patients with CMM. The clinicopathological characteristics of CMM patients, including age, lesion duration, location, pathological classification, Clark’s level, Breslow thickness, metastasis and recurrence, were collected. Immunohistochemical staining and Western blot analysis for HSP105 were performed. Pigmented nevi (n = 20) served as a control. The staining intensity and percentage of stained cells were expressed as a histochemical score (HSCORE).

Results
HSP105 was overexpressed in melanoma compared with nevi. Differences in the HSCORE between nevi (HSCORE = 1.05(0.15,1.50)) and CMM (HSCORE = 2.68(1.80,3.60)) were remarkable (P<0.001). Exposed site lesions, recurrent and metastatic lesions, nodular melanoma and lentigo maligna melanoma were closely associated with higher HSP105 expression (P = 0.011, P = 0.001 and P = 0.001, respectively). Moreover, no significant difference was observed in Clark’s level, Breslow thickness, or lesion duration (P>0.05).

Conclusion
HSP105 is overexpressed in CMM. Higher HSP105 expression in lesions is associated with different clinicopathological variables. HSP105 may be a potential target for the diagnosis, treatment and prognostic prediction of CMM.
Risk factors for microbiologic failure in children with Enterobacter species bacteremia
Authors: Boguniewicz Juri , Revell Paula A. , Scheurer Michael E. , Hulten Kristina G. , Palazzi Debra L. 
Published: October 2021

Background
Enterobacter species are an important cause of healthcare-associated bloodstream infections (BSI) in children. Up to 19% of adult patients with Enterobacter BSI have recurrence of infection resistant to third-generation cephalosporins (3GCs) while on therapy with a 3GC. Data are lacking regarding the incidence of and risk factors for recurrence of infection in children with Enterobacter BSI.

Methods
We conducted a retrospective case-control study of patients aged ≤21 years old admitted to Texas Children’s Hospital from January 2012 through December 2018 with Enterobacter BSI. The primary outcome was microbiologic failure from 72 hours to 30 days after the initial BSI (cases). The secondary outcome was isolation of a 3GC non-susceptible Enterobacter sp. from a patient with an initial 3GC-susceptible isolate.

Results
Twelve patients (6.7%) had microbiologic failure compared to 167 controls without microbiologic failure. Of the 138 patients (77.1%) with an Enterobacter sp. isolate that was initially susceptible to 3GCs, 3 (2.2%) developed a subsequent infection with a non-susceptible isolate. Predictors of microbiologic failure were having an alternative primary site of infection besides bacteremia without a focus or an urinary tract infection (OR, 9.64; 95% CI, 1.77–52.31; P < 0.01) and inadequate source control (OR, 22.16; 95% CI, 5.26–93.36; P < 0.001).

Conclusions
Source of infection and adequacy of source control are important considerations in preventing microbiologic failure. In-vitro susceptibilities can be used to select an antibiotic regimen for the treatment of Enterobacter BSI in children.
Long-term results of hypofractionation with concomitant boost in patients with early breast cancer: A prospective study
Authors: Saksornchai Kitwadee , Jaruthien Thitiporn , Nantavithya Chonnipa , Shotelersuk Kanjana , Rojpornpradit Prayuth 
Published: October 2021

Aim
To report the long-term local control and survival of patients with early breast cancer who had hypofractionated whole breast irradiation with concomitant boost (Hypo-CB).

Methods and materials
Between October 2009 and June 2010, 73 patients with early breast cancer (T1-3N0-1M0) who underwent breast conserving surgery were enrolled into the study. Thirty-six of these participants received 50 Gy of conventional irradiation in 25 fractions over 5 weeks to the whole breast with a sequential boost to the tumor bed with 10–16 Gy in 5–8 fractions (Conv-SEQ). The other 37 participants received a hypofractionated dose of 43.2 Gy in 16 fractions with an additional daily concomitant boost (CB) of 0.6 Gy over 3 weeks (Hypo-CB).

Results
At a median follow-up time of 123 months, ipsilateral local recurrence (ILR) was found in 3 participants, 1 of whom was in the hypofractionated group. All 3 ILR were true local recurrence (TR). There were no significant differences in the 10-year disease free survival (DFS) and 10-year overall survival rates (OS) between the conventional and hypofractionated groups (93.9% vs. 94.4%, p = 0.96 and 91.9% vs. 91.6%, p = 0.792, respectively).

Conclusion
This study showed that the effectiveness, DFS and OS were comparable between hypofractionated whole breast irradiation with a CB and the conventional irradiation with a sequential boost.
The activity of markers of oxidative and nitrosative stresses in blood plasma of Parkinson’s disease patients at the early stages
Authors: Demchenko A. V. , Biriuk V. V. , Abramov A. V. 
Published: October 2021

The aim of the study is to investigate activity of markers of oxidative and nitrosative stresses in blood plasma of patients in the I–II stages of Parkinson’s disease (PD) and to determine correlations between their concentrations and severity of non-motor PD symptoms.
Materials and methods. 67 patients at I–II PD stages and 20 healthy controls took part in the research. Cognitive functions were examined due to the Montreal Cognitive Assessment test – MoCA test. For the severity of psycho-emotional disorders evaluation the following scales and questionnaires were used: Night Sleep Assessment Questionnaire by A. M. Vein, Zung test for anxiety, apathy Starkstein scale, Boston stress-resistance test, Beck Depression Inventory (BDI-II). We performed ELISA test for determination of glutathione peroxidase (GPx) and glutathione-S-transferase (GST) activities and 3-nitrotyrosine (3-NT) level in blood plasma of participants (Elabscience® kit).
Results. The middle age of PD patients and healthy controls was 64.35 ± 1.22 and 66.40 ± 0.70 years, respectively. GPx activity in plasma of patients at І–ІІ PD stages was significantly lower than in healthy controls (P < 0.001) and was higher at the I stage compared to the II PD stage (P = 0.003). Also GPx activity in PD patients with normal cognition was higher than in PD patients with cognitive impairment (P = 0.042).
The GST activity in plasma of PD patients with anxiety was significantly lower (P = 0.002) compared to those without anxiety, and 3-NT blood plasma level in PD patients with moderate anxiety was higher than in those without one (P = 0.029).
Conclusions. The activity of antioxidant GPx was significantly lower in PD patients at early stages compared to healthy controls, and in PD patients in the II stage of the disease compared to the I stage, and it was significantly lower in PD patients with cognitive impairment. PD patients with moderate anxiety had lower 3-NT levels and GST activity in blood plasma.
Pathomorphological and immunohistochemical features of the esophageal mucosa in children with asthma combined with reflux esophagitis
Authors: Zadorozhna T. D. , Antypkin Yu. H. , Umanets T. R. , Buratynska A. A. , Archakova T. M. , Kiurdzhiieva M. I. , Kylykhevych S. M. 
Published: October 2021

The most common comorbid pathology in children with asthma is gastroesophageal reflux disease (GERD) associated with reflux esophagitis (RE), which ranges from 32 % to 80 %. Histological changes of the esophageal mucosa in RE have been described in adults and children, but there are only isolated studies that describe the morphological features of the esophageal mucosa in combined pathology, taking into account the severity of asthma.
The aim is to study the histological and immunohistochemical features of the esophageal mucosa in children with asthma combined with reflux esophagitis.
Materials and methods. In 43 children aged 6–17 years with RE and severe asthma (group 1), with mild/moderate asthma (group 2) and without asthma (group 3), mucosal biopsies from the distal and proximal esophagus were examined by histolo­gical and immunohistochemical methods. Immunohistochemical research was performed in serial paraffin sections according to standard protocols using monoclonal antibodies to Ki-67, MMP-9, VEGF, BCL-2, IgE and CD68.
Results. In children with asthma combined with RE, the structural changes of the esophageal mucosa differed depending on the severity of asthma. Severe basal epithelial hyperplasia was registered in 92.31 % of children in group 1, and its frequency was significantly different from children in groups 2 and 3. A characteristic feature of children with asthma and reflux eso­phagitis was dyschronosis, which was characterized by foci of hypo- and hypertrophy of basal layer cells (92.31 % of children in group 1 and 37.50 % of children in group 2). Severe elongation of the “papillae” was found only in 38.46 % of children in group 1. Immunohistochemical features of the esophageal mucosa of children with asthma and reflux esophagitis revealed the expression of Ki-67 antigen, IgE and a weak macrophage response (CD68), the severity of which differed from children with reflux esophagitis without asthma.
Conclusions. Morphological changes in the esophageal mucosa of children with reflux esophagitis and asthma differ from children with reflux esophagitis without asthma in the presence of severe epithelial damage, dyschronosis of changes, severe elongation of the “papillae”, pronounced cell proliferation (Ki-67) and local IgE expression.
Ultrasound examination as a method of early “bedside” diagnosis of acute kidney injury in geriatric patients after urgent abdominal surgery
Authors: Momot N. V. , Tumanska N. V. , Vorotyntsev S. I. 
Published: October 2021

Geriatric patients are particularly vulnerable to the development of postoperative complications, one of which is acute kidney injury (AKI). Early diagnosis of AKI is an important component of the effectiveness of its prevention and treatment. It is believed that ultrasound examination of renal blood flow may be a promising method for bedside diagnosis of AKI.
The aim is to evaluate the predictive value of renal resistance index (RRI) and semiquantitative renal perfusion (SQP) as methods for early diagnosis of AKI in geriatric patients after urgent abdominal surgery.
Materials and methods. A prospective single-center study included 40 patients (72.5 (65; 81.5) y. o.) who were assessed for the risk of development, the presence and stage of AKI, and additionally measured hemodynamic parameters, intra-abdominal pressure (IAP), and abdominal perfusion pressure (APP). Renal resistance index (RRI) and semi-quantitative renal perfusion score (SQP) were determined using Doppler ultrasound (ultrasound probe CH-6, SIEMENS, Acuson Antares). Statistical analysis was performed using the STATISTICA for Windows 13 program (StatSoftInc., No. JPZ804I382130ARCN10-J).
Results. In the postoperative period, AKI developed in 26 patients (65%). Compared with patients without AKI, they had a 14% lower level of mean arterial pressure (MAP) (p=0.008), as well as an average of 4 mmHg higher IAP (p=0.005) and significantly lower APP (p=0.0348). Correlation analysis revealed a weak relationship between the values of MAP, IAP, APP and the development of AKI (r=0.34, r=0.41, r=0.392, respectively, p˂0.05).
Patients with AKI had a 13.9% higher RRI than patients without AKI (0.75 (0.72; 0.81) r.u. vs 0.66 (0.61; 0.69) r.u., respectively, p=0.000001), however, the degree of SQP of the kidneys did not differ significantly between the groups (p=0.636). The correlation between the RRI and the development of AKI was of moderate strength (r=0.57, p˂0.05). MAP, IAP, and APP were significantly very weakly correlated with RRI (r=0.34; r=0.41, r=0.392, respectively, p˂0.05). ROC analysis showed that RRI>0.71 r.u. is the threshold level for AKI with 87.2% sensitivity and 73.5% specificity, and it has a high predictive value (area under the curve AUC 0.868 (p<0.0001)).
Conclusions. High RRI values are associated with AKI in geriatric patients after urgent abdominal surgery. RRI may be a bedside diagnostic tool for AKI, in contrast to renal SQP, which has shown no predictive value.
Hindlimb suspension in Wistar rats: Sex‐based differences in muscle response
Authors: Mortreux Marie , Rosa‐Caldwell Megan E. , Stiehl Ian D. , Sung Dong‐Min , Thomas Nicholas T. , Fry Christopher S. , Rutkove Seward B. 
Published: October 2021

AbstractGround‐based animal models have been used extensively to understand the effects of microgravity on various physiological systems. Among them, hindlimb suspension (HLS), developed in 1979 in rats, remains the gold‐standard and allows researchers to study the consequences of total unloading of the hind limbs while inducing a cephalic fluid shift. While this model has already brought valuable insights to space biology, few studies have directly compared functional decrements in the muscles of males and females during HLS. We exposed 28 adult Wistar rats (14 males and 14 females) to 14 days of HLS or normal loading (NL) to better assess how sex impacts disuse‐induced muscle deconditioning. Females better maintained muscle function during HLS than males, as shown by a more moderate reduction in grip strength at 7 days (males: −37.5 ± 3.1%, females: −22.4 ± 6.5%, compared to baseline), that remains stable during the second week of unloading (males: −53.3 ± 5.7%, females: −22.4 ± 5.5%, compared to day 0) while the males exhibit a steady decrease over time (effect of sex × loading p = 0.0002, effect of sex × time × loading p = 0.0099). This was further supported by analyzing the force production in response to a tetanic stimulus. Further functional analyses using force production were also shown to correspond to sex differences in relative loss of muscle mass and CSA. Moreover, our functional data were supported by histomorphometric analyzes, and we highlighted differences in relative muscle loss and CSA. Specifically, female rats seem to experience a lesser muscle deconditioning during disuse than males thus emphasizing the need for more studies that will assess male and female animals concomitantly to develop tailored, effective countermeasures for all astronauts.
Generalised Beauville groups
Authors: Carta Ludo , Fairbairn Ben T. 
Published: October 2021

Abstract
A Beauville surface is defined by the action of a finite group (Beauville group) on the product of two compact Riemann surfaces.
In this paper, we consider higher products and the possibility of a similar action by a finite group, which we call a generalised Beauville group; we prove several results regarding the existence and construction of infinite families of generalised Beauville groups and provide a complete classification of the abelian ones; we list all generalised Beauville groups of orders from 1 to 1023.
Editorial Introduction to VIBE Special Issue
Authors: McAskill Ash , Sawchuk Kim , Thulin Samuel 
Published: October 2021

This special issue of the Canadian Journal of Disability Studies is a result of the activity surrounding VIBE: Challenging ableism and audism through the arts, a 3-day international symposium exploring the existing and potential contributions of the Deaf/disability arts to aesthetic innovations, research-creation and cultural change in attitudes towards the capacities of the Deaf/disabled. The symposium, which took place at Concordia University from November 30 - December 2, 2018, brought together Deaf/disabled academics, emerging scholars, post-doctoral researchers, activists, artists, and students – and their allies – for vibrant exchanges on the relationship between disability arts research and disability arts practice.
DEVELOPMENT OF OPTIMAL COMPOSITION FORMULATION OF RESVERATROL AND SOLUBILISERS
Authors: Krechetov S. P. , Maslennikova M. S. , Solovieva N. L. , Krasnyuk I. I. 
Published: October 2021

Introduction. Increasing of poorly soluble pharmaceutical substances bioavailability is one of important problems of pharmaceutical technology. Resveratrol is a plant origin polyphenol with a broad spectrum of biological effects. However, due to poor solubility and, as a result, low bioavailability, it is not promising for the development of oral drugs. Thus, today resveratrol is presented only as a biologically active substance that is component of biologically active food supplements.The objective of the research is selection of the optimal solubilizer to increase the solubility of resveratrol by determining the solubilization parameters.Materials and methods. The spectrophotometric characteristics of resveratrol were studied using three groups of solubilizers: poloxamers, polysorbates and cyclodextrins. Studies were carried out in 50 mM hydrochloric acid buffer(pH 1.2) and 50 mM phosphate buffer(pH 6.8). Spectrophotometric measurements were carried out on a spectrophotometer UV/VIS-3600 Shimadzu (Japan) in the wavelength range of 220–380 nm. The effect of solubilizers on the spectrophotometric characteristics of resveratrol was determined in buffer solutions containing the solubilizer and resveratrol in significantly less concentration of its own solubility in water. The used multiple excess of the solubilizer ensured the finding of all resveratrol in a solubilized form. During the determining parameters of solubilization, buffer solutions containing from 2 to 10 mM solubilizers were added to the obviously excess of resveratrol. The indicated amount of resveratrol ensured the presence of its precipitate in all experimentsto determine the completeness of solubilization of the studied polyphenol.Results. Based on the obtained spectrophotometric characteristics of solubilizers solutions with resveratrol, the most effective for the further development of solid dosage forms for oral administration are poloxamer 407, polysorbate 80 and modified methyl-beta-cyclodextrin, which ensure complete dissolution of resveratrol when its content in the composition with a solubilizer is about 10 %.Conclusion. Based on the data obtained on the spectrophotometric characteristics of resveratrol using solubilizers, it can be argued that it is possible to create drugs with improved solubility of the studied polyphenol. On the basis of its compositions with poloxamer 407, polysorbate 80 and modified methyl-beta-cyclodextrin, with the selection of appropriate excipients, solid dosage forms for oral intake can be developed.
Atividade física para mulher portadora de síndrome de Churg Strauss: relato de caso
Authors: Moura Dagnou Pessoa de 
Published: October 2021

Introdução: A síndrome de Churg Strauss (SCS) é uma doença autoimune de etiologia indeterminada. Seu diagnóstico é difícil, não somente pela raridade, mas também pela sobreposição clínica e anatomopatológica que pode haver entre diferentes vasculites; por este motivo, pode ter sua prevalência subestimada. Manifestações sistêmicas incluem mialgia, febre, perda de peso, artralgia, alterações cutâneas, neuropatia periférica, envolvimento pulmonar, de trato gastrintestinal e cardiomiopatia. Comumente são utilizados glicocorticoides para combater os efeitos da doença, entretanto, apresentam efeitos colaterais importantes, como obesidade centrípeta, face em lua cheia, giba ou corcunda de búfalo, osteoporose, fraqueza, miopatia, atrofia muscular; necrose asséptica da cabeça do fêmur e úmero, além da depressão do sistema imunológico. Objetivo: Relatar um caso singular de uma paciente diagnosticada com SCS por 10 anos, que realiza tratamento com corticosteroides e treinamento físico para combater os efeitos colaterais do tratamento farmacológico. Relato de caso: Para combater os efeitos colaterais desse tratamento, foi adotado o treinamento de força (TF) e corrida. A paciente realizou TF três a cinco vezes por semana durante todo o período de tratamento até o momento. A carga utilizada em seus treinamentos variou entre 50 a 70% de 1 Repetição Máxima, de acordo com a periodização, além de corridas duas vezes por semana com percepção subjetiva de esforço entre 4 e 6. Regularmente foram realizados exames de sangue para análise de eosinófilos, densitometria óssea e avaliações antropométricas. O treinamento mostrou indícios de ser útil na manutenção da composição corporal e da densidade mineral óssea (DMO). Conclusão: O TF e o treinamento aeróbio são estratégias importantes no combate aos efeitos da SCS e do tratamento com corticosteroides.
Peculiarities of administration of medications to patients with circulatory system diseases within the framework of the federal program of preferential provision of medicines
Authors: Petrukhina I. K. , Yagudina R. I. , Veyner N. V. , Ryazanova T. K. , Gladunova E. P. , Kurkin V. A. , Blinkova P. R. , Zaitseva E. N. 
Published: October 2021

Objective:to analyse of medicines prescriptions for federal beneficiaries with circulatory system diseases and to identify the main trends in the implementation of the federal program for the provision of necessary medicines in the Samara Region in 2014–2017.Material and methods.The authors reviewed the nomenclature of medicines used to treat circulatory system diseases (CSDs) and distributed to the population of the Samara Region as part of the federal program for the provision of necessary medicines for the period from 2014 to 2017. The methods of comparative, retrospective, logical, graphical and content analysis, the method of data grouping according to Anatomical Therapeutic Chemical classification and methods of descriptive statistics were used.Results.In 2014–2017 the share of financial costs for the purchase of medicines in the total budget of the federal program for the provision of necessary medicines in the Samara Region did not exceed 6%. Still, in physical terms (in terms of the number of packages) the share of medicines of this pharmacotherapeutic group in the federal program for the provision of necessary medicines averaged about 25%. Nomenclature of medicines of the analyzed group prescribed to federal beneficiaries in 2014–2017 ranged from 90 to 107 nomenclature items. The number of international nonproprietary names (INN) decreased from 51 in 2014 to 36 in 2017. During the period under review, there was a decrease in the acquisition cost of medicines for the treatment of circulatory system diseases and the weighted average cost of one package (by 63% and 53%, respectively).Conclusion.Perindopril-containing medicines occupied the maximum consumption volumes in monetary terms. There was a decrease in the average cost of one package in 2017, which may be associated with the replacement of original medicines with generics and a concomitant reduction in the number of INN within the subgroup.