bluuebunny/crossref_metadata_2025_split huggingface dataset:
HIF-1α promotes cellular growth in lymphatic endothelial cells exposed to chronically elevated pulmonary lymph flow
Authors: Boehme Jason T. , Morris Catherine J. , Chiacchia Samuel R. , Gong Wenhui , Wu Katherine Y. , Kameny Rebecca J. , Raff Gary W. , Fineman Jeffrey R. , Maltepe Emin , Datar Sanjeev A. 
Published: January 2021

AbstractNormal growth and development of lymphatic structures depends on mechanical forces created by accumulating interstitial fluid. However, prolonged exposure to pathologic mechanical stimuli generated by chronically elevated lymph flow results in lymphatic dysfunction. The mechanisms that transduce these mechanical forces are not fully understood. Our objective was to investigate molecular mechanisms that alter the growth and metabolism of isolated lymphatic endothelial cells (LECs) exposed to prolonged pathologically elevated lymph flow in vivo within the anatomic and physiologic context of a large animal model of congenital heart disease with increased pulmonary blood flow using in vitro approaches. To this end, late gestation fetal lambs underwent in utero placement of an aortopulmonary graft (shunt). Four weeks after birth, LECs were isolated and cultured from control and shunt lambs. Redox status and proliferation were quantified, and transcriptional profiling and metabolomic analyses were performed. Shunt LECs exhibited hyperproliferative growth driven by increased levels of Hypoxia Inducible Factor 1α (HIF-1α), along with upregulated expression of known HIF-1α target genes in response to mechanical stimuli and shear stress. Compared to control LECs, shunt LECs exhibited abnormal metabolism including abnormalities of glycolysis, the TCA cycle and aerobic respiration. In conclusion, LECs from lambs exposed in vivo to chronically increased pulmonary lymph flow are hyperproliferative, have enhanced expression of HIF-1α and its target genes, and demonstrate altered central carbon metabolism in vitro. Importantly, these findings suggest provocative therapeutic targets for patients with lymphatic abnormalities.
The calming effect of roasted coffee aroma in patients undergoing dental procedures
Authors: Pachimsawat Praewpat , Tangprasert Kanlayanee , Jantaratnotai Nattinee 
Published: January 2021

AbstractCoffee beverage consumption is well-known to exert various health benefits; however, the effects of coffee aroma are rarely explored. This study aimed to investigate the calming effect of inhaling coffee aroma while the patients underwent dental procedures (probing and scaling). Salivary α-amylase (sAA) and cortisol (sCort) levels were measured as proxies of sympathetic nervous system and hypothalamic–pituitary–adrenal axis responses to stress respectively. Blood pressures and pulse rates were recorded. The results showed that undergoing dental procedures could increase sAA and sCort levels of the patients inhaling sham aroma while those inhaling coffee aroma had significantly decreased sAA and sCort levels (40% and 25% differences, respectively). The pulse rates of those inhaling coffee aroma were also lower. Subjective assessment using visual analog scale was in line with objective measures as well. The preference for coffee aroma or the frequency of coffee drinking had no effect on the sAA and sCort responses. This is the first study to provide evidence on the effect of coffee aroma on sAA and sCort levels in patients undergoing dental procedures.
CT image segmentation for inflamed and fibrotic lungs using a multi-resolution convolutional neural network
Authors: Gerard Sarah E. , Herrmann Jacob , Xin Yi , Martin Kevin T. , Rezoagli Emanuele , Ippolito Davide , Bellani Giacomo , Cereda Maurizio , Guo Junfeng , Hoffman Eric A. , Kaczka David W. , Reinhardt Joseph M. 
Published: January 2021

AbstractThe purpose of this study was to develop a fully-automated segmentation algorithm, robust to various density enhancing lung abnormalities, to facilitate rapid quantitative analysis of computed tomography images. A polymorphic training approach is proposed, in which both specifically labeled left and right lungs of humans with COPD, and nonspecifically labeled lungs of animals with acute lung injury, were incorporated into training a single neural network. The resulting network is intended for predicting left and right lung regions in humans with or without diffuse opacification and consolidation. Performance of the proposed lung segmentation algorithm was extensively evaluated on CT scans of subjects with COPD, confirmed COVID-19, lung cancer, and IPF, despite no labeled training data of the latter three diseases. Lobar segmentations were obtained using the left and right lung segmentation as input to the LobeNet algorithm. Regional lobar analysis was performed using hierarchical clustering to identify radiographic subtypes of COVID-19. The proposed lung segmentation algorithm was quantitatively evaluated using semi-automated and manually-corrected segmentations in 87 COVID-19 CT images, achieving an average symmetric surface distance of $$0.495\pm 0.309$$

0.495
±
0.309

mm and Dice coefficient of $$0.985\pm 0.011$$

0.985
±
0.011

. Hierarchical clustering identified four radiographical phenotypes of COVID-19 based on lobar fractions of consolidated and poorly aerated tissue. Lower left and lower right lobes were consistently more afflicted with poor aeration and consolidation. However, the most severe cases demonstrated involvement of all lobes. The polymorphic training approach was able to accurately segment COVID-19 cases with diffuse consolidation without requiring COVID-19 cases for training.
A novel graphene-based micro/nano architecture with high strength and conductivity inspired by multiple creatures
Authors: Li Muzhi , Wang Xiuya , Zhao Ru , Miao Yuanyuan , Liu Zhenbo 
Published: January 2021

AbstractIn the long history of development and elimination, the creatures have derived a variety of exquisite structures and unique properties, typically natural nacre, marine mussel and Glycera to adapt to the environment and resist the predation of the enemy. Hence, inspired by the combination of special structures and properties of multiple creatures, a novel type of graphene-based micro/nano architecture was proposed, and the related bioinspired nanocomposites were fabricated, Polydopamine coated Graphene oxide/Nanocellulose/Polydopamine (P-GCP). Apart from replicating the layered structure of natural nacre, P-GCP also introduced copper ions and polydopamine to simulate the hardening mechanism of the Glycera’s jaw and the composition of adhesive proteins in mussels to further improve the tensile strength and conductivity of nanocomposites, respectively. The test results showed that the tensile strength of P-GCP reached 712.9 MPa, which was 5.3 times that of natural nacre. The conductivity of artificial nacre was as high as 207.6 S/cm, which was equivalent to that of reduced graphene oxide (rGO). Furthermore, the material exhibited outstanding electrical conductivity when it connected as wires in a circuit, demonstrating the practical application prospects in aerospace, supercapacitors, biomaterials, artificial bones and tissue engineering.
Reconstruction and evaluation of oil-degrading consortia isolated from sediments of hydrothermal vents in the South Mid-Atlantic Ridge
Authors: Ma Meng , Zheng Li , Yin Xiaofei , Gao Wei , Han Bin , Li Qian , Zhu Aimei , Chen Hao , Yang Huanghao 
Published: January 2021

AbstractIn this study, sediments were collected from two different sites in the deep-sea hydrothermal region of the South Atlantic Ocean. Two microbial enrichment cultures (H7S and H11S), which were enriched from the sediments collected at two sample sites, could effectively degrade petroleum hydrocarbons. The bacterial diversity was analyzed by high-throughput sequencing method. The petroleum degradation ability were evaluated by gas chromatography–mass spectrometry and gravimetric analysis. We found that the dominant oil-degrading bacteria of enrichment cultures from the deep-sea hydrothermal area belonged to the genera Pseudomonas, Nitratireductor, Acinetobacter, and Brevundimonas. After a 14-day degradation experiment, the enrichment culture H11S, which was obtained near a hydrothermal vent, exhibited a higher degradation efficiency for alkanes (95%) and polycyclic aromatic hydrocarbons (88%) than the enrichment culture H7S. Interestingly, pristane and phytane as biomarkers were degraded up to 90% and 91% respectively by the enrichment culture H11S, and six culturable oil-degrading bacterial strains were isolated. Acinetobacter junii strain H11S-25, Nitratireductor sp. strain H11S-31 and Pseudomonas sp. strain H11S-28 were used at a density ratio of 95:4:1 to construct high-efficiency oil-degrading consortium H. After a three-day biodegradation experiment, consortium H showed high degradation efficiencies of 74.2% and 65.7% for total alkanes and PAHs, respectively. The degradation efficiency of biomarkers such as pristane and high-molecular-weight polycyclic aromatic hydrocarbons (such as CHR) reached 84.5% and 80.48%, respectively. The findings of this study indicate that the microorganisms in the deep-sea hydrothermal area are potential resources for degrading petroleum hydrocarbons. Consortium H, which was artificially constructed, showed a highly efficient oil-degrading capacity and has significant application prospects in oil pollution bioremediation.
Aqueous autotaxin and TGF-βs are promising diagnostic biomarkers for distinguishing open-angle glaucoma subtypes
Authors: Igarashi Nozomi , Honjo Megumi , Asaoka Ryo , Kurano Makoto , Yatomi Yutaka , Igarashi Koji , Miyata Kazunori , Kaburaki Toshikatsu , Aihara Makoto 
Published: January 2021

AbstractThe purpose of this study is to examine if aqueous autotaxin (ATX) and TGF-β levels could be used for differentiating glaucoma subtypes. This prospective observational study was performed using aqueous humor samples obtained from 281 consecutive patients. Open angle glaucoma patients were classified into three groups: primary open-angle glaucoma (POAG), secondary open-angle glaucoma (SOAG), and exfoliation glaucoma (XFG). Aqueous levels of ATX and TGF-βs were quantified. The AUC as well as sensitivity and specificity for the classification into normal and glaucoma subtypes using four indicators-ATX, TGF-β1, TGF-β2, and TGF-β3, upon the application of three machine learning methods. ATX, TGF-β1, and TGF-β3 were positively correlated with IOP, and ATX was significantly and negatively correlated with the mean deviation. From least absolute shrinkage and selection operator regression analysis, the AUC values to distinguish each subgroup [normal, POAG, SOAG, and XFG] ranged between 0.675 (POAG vs. normal) and 0.966 (XFG vs. normal), when four variables were used. High AUC values were obtained with ATX for discriminating XFG from normal eyes and with TGF-β3 for discriminating XFG from normal eyes, POAG, or SOAG. Aqueous TGF-β and ATX exhibited high diagnostic performance in detecting glaucoma subtypes, and could be promising biomarkers for glaucoma.
The specific DNA barcodes based on chloroplast genes for species identification of Orchidaceae plants
Authors: Li Huili , Xiao Wenjun , Tong Tie , Li Yongliang , Zhang Meng , Lin Xiaoxia , Zou Xiaoxiao , Wu Qun , Guo Xinhong 
Published: January 2021

AbstractDNA barcoding is currently an effective and widely used tool that enables rapid and accurate identification of plant species. The Orchidaceae is the second largest family of flowering plants, with more than 700 genera and 20,000 species distributed nearly worldwide. The accurate identification of Orchids not only contributes to the safe utilization of these plants, but also it is essential to the protection and utilization of germplasm resources. In this study, the DNA barcoding of 4 chloroplast genes (matK, rbcL, ndhF and ycf1) were used to provide theoretical basis for species identification, germplasm conservation and innovative utilization of orchids. By comparing the nucleotide replacement saturation of the single or combined sequences among the 4 genes, we found that these sequences reached a saturation state and were suitable for phylogenetic relationship analysis. The phylogenetic analyses based on genetic distance indicated that ndhF and ycf1 sequences were competent to identification at genus and species level of orchids in a single gene. In the combined sequences, matK + ycf1 and ndhF + ycf1 were qualified for identification at the genera and species levels, suggesting the potential roles of ndhF, ycf1, matK + ycf1 and ndhF + ycf1 as candidate barcodes for orchids. Based on the SNP sites, candidate genes were used to obtain the specific barcode of orchid plant species and generated the corresponding DNA QR code ID card that could be immediately recognized by electronic devices. This study provides innovative research methods for efficient species identification of orchids. The standardized and accurate barcode information of Orchids is provided for researchers. It lays the foundation for the conservation, evaluation, innovative utilization and protection of Orchidaceae germplasm resources.
Changes on proteomic and metabolomic profile in serum of mice induced by chronic exposure to tramadol
Authors: Jiang Shukun , Liu Guojie , Yuan Huiya , Xu Enyu , Xia Wei , Zhang Xiaoyu , Liu Junting , Gao Lina 
Published: January 2021

AbstractTramadol is an opioid used as an analgesic for treating moderate or severe pain. The long-term use of tramadol can induce several adverse effects. The toxicological mechanism of tramadol abuse is unclear. Limited literature available indicates the change of proteomic profile after chronic exposure to tramadol. In this study, we analyzed the proteomic and metabolomic profile by TMT-labeled quantitative proteomics and untargeted metabolomics between the tramadol and the control group. Proteomic analysis revealed 31 differential expressed serum proteins (9 increased and 22 decreased) in tramadol-treated mice (oral, 50 mg/kg, 5 weeks) as compared with the control ones. Bioinformatics analysis showed that the dysregulated proteins mainly included: enzyme inhibitor-associated proteins (i.e. apolipoprotein C-III (Apoc-III), alpha-1-antitrypsin 1–2 (Serpina 1b), apolipoprotein C-II (Apoc-II), plasma protease C1 inhibitor, inter-alpha-trypsin inhibitor heavy chain H3 (itih3)); mitochondria-related proteins (i.e. 14-3-3 protein zeta/delta (YWHAZ)); cytoskeleton proteins (i.e. tubulin alpha-4A chain (TUBA4A), vinculin (Vcl)). And we found that the differential expressed proteins mainly involved in the pathway of the protein digestion and absorption. Metabolomics analysis revealed that differential expressed metabolites mainly involved in protein ingestion and absorption, fatty acid biosynthesis, steroid hormone biosynthesis and bile secretion. Our overall findings revealed that chronic exposure to tramadol changed the proteomic and metabolomic profile of mice. Moreover, integrated proteomic and metabolomic revealed that the protein digestion and absorption is the common enrichment KEGG pathway. Thus, the combination of proteomics and metabolomics opens new avenues for the research of the molecular mechanisms of tramadol toxicity.
Integrated, automated maintenance, expansion and differentiation of 2D and 3D patient-derived cellular models for high throughput drug screening
Authors: Boussaad Ibrahim , Cruciani Gérald , Bolognin Silvia , Antony Paul , Dording Claire M. , Kwon Yong-Jun , Heutink Peter , Fava Eugenio , Schwamborn Jens C. , Krüger Rejko 
Published: January 2021

AbstractPatient-derived cellular models become an increasingly powerful tool to model human diseases for precision medicine approaches. The identification of robust cellular disease phenotypes in these models paved the way towards high throughput screenings (HTS) including the implementation of laboratory advanced automation. However, maintenance and expansion of cells for HTS remains largely manual work. Here, we describe an integrated, complex automated platform for HTS in a translational research setting also designed for maintenance and expansion of different cell types. The comprehensive design allows automation of all cultivation steps and is flexible for development of methods for variable cell types. We demonstrate protocols for controlled cell seeding, splitting and expansion of human fibroblasts, induced pluripotent stem cells (iPSC), and neural progenitor cells (NPC) that allow for subsequent differentiation into different cell types and image-based multiparametric screening. Furthermore, we provide automated protocols for neuronal differentiation of NPC in 2D culture and 3D midbrain organoids for HTS. The flexibility of this multitask platform makes it an ideal solution for translational research settings involving experiments on different patient-derived cellular models for precision medicine.
Changes in lung immune cell infiltrates after electric field treatment in mice
Authors: Eliseeva Sophia I. , Knowlden Zackery A. , Lester Gillian MSchiralli , Dean David A. , Georas Steve N. , Chapman Timothy J. 
Published: January 2021

AbstractExogenous electric fields are currently used in human therapy in a number of contexts. Interestingly, electric fields have also been shown to alter migration and function of immune cells, suggesting the potential for electric field-based immune therapy. Little is known as to the effect of electric field treatment (EFT) on the lung. To determine if EFT associates with changes in lung immune cell infiltration, we used a mouse model with varying methods of EFT application and measured cells and soluble mediators using flow cytometry and cytokine/chemokine multiplex. EFT was associated with a transient increase in lung neutrophils and decrease in eosinophils in naïve mice within 2 h of treatment, accompanied by an increase in IL-6 levels. In order to test whether EFT could alter eosinophil/neutrophil recruitment in a relevant disease model, a mouse model of allergic airway inflammation was used. Four EFT doses in allergen-sensitized mice resulted in increased neutrophil and reduced eosinophil infiltrates following allergen challenge, suggesting a durable change in inflammation by EFT. Mice with allergic inflammation were analyzed by flexiVent for measures of lung function. EFT-treated mice had increased inspiratory capacity and other measures of lung function were not diminished. These data suggest EFT may be used to manipulate immune cell infiltration in the lung without affecting lung function.
Discrete and polymeric organometallic-organic assemblies based on the diarsene complex [(Cp)2Mo2(CO)4(μ,η2-As2)], AgPF6and N-donor organic molecules
Authors: Moussa Mehdi Elsayed , Schiller Jana , Seidl Michael , Shelyganov Pavel A. , Scheer Manfred 
Published: January 2021

The first mixed-ligand self-assembly reactions of the diarsene complex [Cp2Mo2(CO)4(μ,η2-As2)] and N-donor organic molecules in the presence of AgPF6 allow for the synthesis of two discrete and four polymeric supramolecular aggregates.
Chronobiologische Ernährung – wann ist es Zeit zu essen? – Dinner Cancelling oder Breakfast Skipping?
Authors: Stange Rainer 
Published: January 2021

SummaryOb eine Kalorie am Morgen einer Kalorie am Abend entspricht, steht derzeit in der Diskussion. Hintergrund istder Zusammenhang zirkadianer Rhythmen mit Ernährungund Stoffwechsel. Frühe Forschungsergebnissehaben gezeigt, dass endokrinologische und thermogenetischeReaktionen auf die Energiezufuhr von der Tageszeitabhängen. Daraus resultierte u. a. die Beobachtung,dass eine Reduktionskost bei Übergewichtigen zugrößeren Gewichtsverlusten führt, wenn der Schwerpunktder Energieaufnahme morgens liegt.Fastenformen wie intermittierendes Fasten, Intervallfastenoder Frühstücksfasten erfreuen sich derzeit großer Beliebtheit, während noch viele Fragen im Sinne der EBM offen sind. Dennoch kann Intervallfasten in der Praxisdem jeweiligen Chronotyp entsprechend unter Beobachtungrelativ einfach dokumentierbarer klinischer Parameter angewendet werden. Erfolge stellen sich beitäglich praktiziertem Intervallfasten meist in 2–3 Wochenein.
Predictors of the Size and Surgical Freedom of the Trans-Cribriform and Trans-Clival Corridors, a Radiographic Analysis
Authors: Aljuboori Zaid , Nuru Mohammed , Sharma Mayur , Andaluz Norberto 
Published: January 2021

Abstract
Introduction The transcribriform and transclival corridors are endoscopic endonasal approaches used to treat pathologies of the skull base. We present a predictive model that uses the clival length and ethmoidal width to predict the size and surgical freedom (SF) of these corridors.
Methods Adult facial computed tomography scans were reviewed. Exclusion criteria included patients <18 years of age or radiographic evidence of trauma, neoplasm, or congenital deformities of the skull base. The images were analyzed using OsiriX MD (Bernex, Switzerland). Patients' demographics, clival length, ethmoidal width, surface area, and others were collected. Linear regression was used to create prediction models for the size and SF of the transclival and transcribriform corridors.
Results A total of 103 patients were included with an average age of 44.9 years and 47% males. Females had a smaller clival surface area (8 vs. 9.2 cm2, p = 0.001). For transclival corridor, clival length correlated positively with SF in the sagittal plane (rho = 0.44, p < 0.05) and negatively with SF in the coronal plane (rho = − 0.2, p < 0.05). For transcribriform corridor, ethmoidal width correlated positively with SF in the coronal plane (rho = 0.74, p < 0.05), and negatively with SF in the sagittal plane (rho = − 0.2, p < 0.05).
Conclusion A significant variability of the bony anatomy of the anterior and central skull base was found. The use of clival length and ethmoidal width as part of preoperative surgical planning might help to overcome the anatomical variability which could affect the adequacy of surgical corridors.
Aggressive Treatment Including Endonasal Surgical Sequestrectomy with Vascularized Nasoseptal Flap Can Improve Outcomes of Skull Base Osteoradionecrosis
Authors: Cho Sung-Woo , Han Sang Y. , Song Yoonjae , Kim Jeong-Whun , Kim Hyun J. , Kim Dong-Young , Rhee Chae-Seo , Bae Yun J. , Kim Ji-hoon , Wu Hong-Gyun , Bang Jae S. , Won Tae-Bin 
Published: January 2021

Abstract
Objective Skull base osteoradionecrosis (SB-ORN) is a serious, potentially lethal complication of radiation therapy. We aimed to review the clinical characteristics and outcomes of SB-ORN according to the extent of treatment.
Design Retrospective analysis design was used for this study.
Setting The study was conducted in two tertiary care hospitals.
Participants Patients included who had been clinically diagnosed with SB-ORN from January 2006 to 2017.
Main Outcome Measures Clinical characteristics, including demographics, predisposing factors, presenting symptoms, radiological findings, treatment modalities, and treatment outcomes, were reviewed. Treatment was classified into conservative and aggressive types. Aggressive treatment included radical surgical removal of soft tissue and bony sequestrum with the placement of vascularized tissue. Treatment outcome was analyzed in terms of clinical control, survival, and carotid artery blow out.
Results Fifteen patients (11 males and 4 females) were identified during the study period. Eight patients were managed conservatively, whereas seven patients were managed with aggressive treatment. The 2-year survival was 75% in the aggressive treatment group and 15% in the conservative group (log-rank, p = 0.049). The estimated 2-year blow out free rate was 46.7% for the conservative group and 100% for the aggressive group (log-rank, p = 0.100).
Conclusion In patients with SB-ORN, aggressive management, including surgical removal of sequestrum and coverage with a pedicled flap, is associated with increased survival.