bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Outcome of Bisphosphonate-Related Osteonecrosis of the Jaw&nbsp;Patients’ Gingiva-Derived Mesenchymal Stem Cells&nbsp;on&nbsp;Wound Healing&nbsp;in a Mice Excisional Skin Model.
Authors: Li Mengyu , Yu Yejia , Wang Jiajia , Zhou Yuqiong , Shi Yueqi , Zhang Wenjie , Son Geehun , Ge Jing , Zhao Jun , Zhang Zhiyuan , Yang Chi , Wang Shaoyi 
Published: January 2021

Abstract
Background: Retarded gingival healing is the hallmark of bisphosphonate-related osteonecrosis of the jaw (BRONJ) and poses a great challenge to maxillofacial surgeons. Although previous studies have showed that bisphosphonates (BPs) are highly toxic to healthy gingival mesenchymal stem cells (GMSCs) in vitro, there is overall lack of direct evidence demonstrating the regeneration capacity of oral mucosa in BRONJ patients. In present study, we aim to isolate GMSCs from BRONJ patients’ gingiva and assessed their phenotypes and functions in vitro, as well as their therapeutic effects for wound healing in a mice excisional skin model. Methods: BRONJ patients’ gingival samples were used for microarray analysis, histological detection and cell culture. The stem cells isolated from the central gingiva (center-BRONJ GMSCs) and the peripheral lesions (peri-BRONJ GMSCs) were analyzed by Cell Counting Kit-8 (CCK-8), cell adhesion, scratch and flow cytometry. Luciferase/GFP (Green Fluorescent Proteins)-labeled GMSCs combined with Hydrogel were transplanted in a mice excisional skin model, and mice were divided into a hydrogel alone group, a hydrogel/control GMSCs group, a hydrogel/center-BRONJ GMSCs group and a hydrogel/peri-BRONJ GMSCs group. Bioluminescence imaging trace cell survival in vivo. Healing effects were evaluated by wound area measurement, histology, immunohistochemistry (IH) and immunofluorescence (IF). Results: Center-BRONJ GMSCs and peri-BRONJ GMSCs were all fibroblast-like cells, but they became slender and more wrinkled compared control GMSCs. Notably, they exhibited decreased proliferation, adhesion, migration capacities and underwent early apoptosis in vitro. In animal model, BRONJ GMSCs transplantation also displayed lower cell survival rate and poor healing effects than that of control group. Mechanistically, we found that the expression of TGF-β1 signaling pathway was suppressed not only in BRONJ patients’ gingival lesions but also in BRONJ GMSCs transplantation animal model. Conclusions: In BRONJ patients’ microenvironment, the regeneration ability of oral mucosa was dramatically decreased. Our mice skin model demonstrated for the first time that BRONJ GMSCs transplantation displayed poor effects on wound healing mainly via suppressing TGF-β1 signaling pathway. This study provides new insights into the prevention for BRONJ by improving the functions of GMSCs in accelerating gingival wound healing.
Upper/Lower Respiratory Tract Microbiome Changes in AECOPD and COPD
Authors: Xu Yanyan , Liu Nailiang , Yan Dailing , Zhou Shiyi , Tian Kun , Duan Xingqiu , Yang Xiaohui , Huang Zhenming , Cai Bo , Su Jianyuan , zhang Jingxin , Qin Jian , Yang Gang , Pan Quan , Yuan Bing , Zhang Yunhui 
Published: January 2021

Abstract
Background: Microbiome residing in the respiratory tract has emerged as an important player in the etiology and progression of COPD, but results are conflicting regarding the features of respiratory tract microbiome in COPD and at exacerbations and it is unknown whether these features differ by ethnicity and geography. Method: To address these questions, we enrolled healthy individuals and patients with COPD, including healthy-COPD pairs from same households, from four geographical regions of Yunan province, representative of different ethnicities and/or environmental exposures. Sputum and oropharyngeal swabs were collected from these healthy individuals and from COPD patients at stable state (COPD) or exacerbations (AECOPD) and subjected to 16S microbiome profiling. Results: Both COPD disease status and region had an impact on alpha-diversity of sputum and oropharyngeal microbiomes, with AECOPD having the lowest microbiome diversity. Shifts in the relative abundance (≥ 1.5 fold, adj. p < 0.05) of microbes at healthy, exacerbation and stable COPD. Microbes enriched at exacerbation COPD were primarily Proteobacteria and Firmicutes phylum in upper respiratory tract. In the lower respiratory tract, population-based study did not find any statistical differential abundance of microbe, however, paired-based study showed phylum of Proteobacteria, Chloroflexi, Bacteroidota, Acidobacteriota, Desulfobacterota, Firmicutes and Verrucomicrobiota enriched in exacerbation COPD. Conclusions: COPD is associated with specific changes in upper and lower respiratory tract microbiomes, whereas exacerbation events confer additional alterations in respiratory tract microbiome compositions. These specific microbiome changes may inform future study on the pathogenesis or management of COPD.
Alleviation of Disease Activity in Lupus mice by Blocking Secondary Pyroptosis of Renal Tubular Epithelial Cells
Authors: Luo Guihu , Yang Fangyuan , Zhai Zeqing , He Yi , Han Jiaochan , Zhuang Lili , Zhang Yanang , Li Yehao , Song Rui , Luo Xiaoqing , Liang Jianheng , Sun Erwei 
Published: January 2021

Abstract
Background:Increased apoptosis and/or defect in clearance apoptotic cells resulting in massive secondary necrosis have been recognized as the main causes of systemic lupus erythematosus. Recent findings have revealed that GSDME, a membrane pore protein downstream of caspase-3, is a key mediator of secondary pyroptosis and cellular membrane disruption. Here, we aim to investigate the effects of secondary pyroptosis on disease activity in lupus mice. Methods:In vivo, Pristane-induced lupus mice were treated with JNK inhibitor SP600125 or vehicle. The disease severity was evaluated and the expression of GSDME and cleaved-caspase-3 detected. In vitro, HK2 human tubular epithelial cells were pretreated with SP600125, followed by treatment with apoptosis inducer, TNF+CHX, or SLE sera. And then the secondary pyroptosis were examined. Results:In vivo, high levels of GSDME expression were revealed in renal tubules in PIL mice and SLE patients. In lupus mice, SP600125 administration effectively ameliorated lupus-like features and importantly, reduced the expression of GSDME and cleaved caspase-3 in renal tubules. In vitro, treatment with TNF+CHX or SLE sera induced HK2 cells to undergo secondary pyroptosis in a caspase-3-GSDME dependent manner. Likewise, SP600125 significantly reduced GSDME expression and decreased the secondary pyroptosis in HK2 cells. Conclusions:The GSDME-mediated pyroptosis may be one of the pathogenesis of SLE and targeting GSDME may be a potential strategy for treating SLE.
Regulation of abnormal expression of hsa_circRNA_0127523 on proliferation, migration and invasion and miR-515-5p expression in oral squamous cell carcinoma
Authors: Wei Jianing , Wang Zekun , Gao Jiping , Liang Yuxiang , Xu Guoqiang , Wang Xiaotang , Xiao Lanfei , Yan Xiaoru , Song Guohua 
Published: January 2021

Abstract
Circular RNA (circRNA) play an important role in many biological processes of occurrence and development of cancers. In this research, we established an animal model by DMBA-induced buccal sac tissue and discovered the differential expression of circRNAs by the second-generation sequencing. Models were identified by HE staining and ultrastructural observation. We found total of 3485 circRNAs were differentially expressed which the top 8 different expression circRNAs in sequencing results were confirmed by qRT-PCR. GO and KEGG analyses showed that differently expressed circRNAs function and pathway. Moreover, the axis relationship of circRNA-miRNA was predicted by miRanda. Silencing hsa_circ_0127523 significantly suppressed the proliferation, migration and invasion abilities of oral squamous cell carcinoma (OSCC) cells by RNAi, CCK-8, transwell migration and invasion assays. Furthermore, bioinformatics databases suggested that hsa_circ_0127523 might function by sponging miR-515-5p to regulate the expression of TRIP13. It was subsequently identified that hsa_circ_0127523 may inhibit miR-515-5p expression and increase TRIP13 expression in the OSCC cells. In summary, the results of the present study revealed that OSCC tissues have abundant circRNAs and we firstly explore the role of the hsa_circ_0127523 in the behavior regulation of OSCC cells. Its demonstrating that hsa_circ_0127523 may be a potential biomarker and therapeutic target of OSCC.
Attitudes Toward a Proposed GPS-Based Location Tracking Smartphone App for Improving Engagement in HIV Care Among Pregnant and Postpartum Women in South Africa: Focus Group and Interview Study
Authors: Clouse Kate , Phillips Tamsin K , Mogoba Phepo , Ndlovu Linda , Bassett Jean , Myer Landon 
Published: January 2021

Background
Peripartum women living with HIV in South Africa are at high risk of dropping out of care and are also a particularly mobile population, which may impact their engagement in HIV care. With the rise in mobile phone use worldwide, there is an opportunity to use smartphones and GPS location software to characterize mobility in real time.

Objective
The aim of this study was to propose a smartphone app that could collect individual GPS locations to improve engagement in HIV care and to assess potential users’ attitudes toward the proposed app.

Methods
We conducted 50 in-depth interviews (IDIs) with pregnant women living with HIV in Cape Town and Johannesburg, South Africa, and 6 focus group discussions (FGDs) with 27 postpartum women living with HIV in Cape Town. Through an open-ended question in the IDIs, we categorized “positive,” “neutral,” or “negative” reactions to the proposed app and identified key quotations. For the FGD data, we grouped the text into themes, then analyzed it for patterns, concepts, and associations and selected illustrative quotations.

Results
In the IDIs, the majority of participants (76%, 38/50) responded favorably to the proposed app. Favorable comments were related to the convenience of facilitated continued care, a sense of helpfulness on the part of the researchers and facilities, and the difficulties of trying to maintain care while traveling. Among the 4/50 participants (8%) who responded negatively, their comments were primarily related to the individual’s responsibility for their own health care. The FGDs revealed four themes: facilitating connection to care, informed choice, disclosure (intentional or unintentional), and trust in researchers.

Conclusions
Women living with HIV were overwhelmingly positive about the idea of a GPS-based smartphone app to improve engagement in HIV care. Participants reported that they would welcome a tool to facilitate connection to care when traveling and expressed trust in researchers and health care facilities. Within the context of the rapid increase of smartphone use in South Africa, these early results warrant further exploration and critical evaluation following real-world experience with the app.
Head and Neck Tumors in Children: Part II
Authors: Yu Jason W. , Lopez Joseph , Afshar Salim 
Published: January 2021

The following chapter will focus on common soft tissue lesions one would encounter as pediatric head and neck tumors. The selected topics covered will be cholesteatoma, craniopharyngioma, encephalocele, teratomas, dermoid cysts and pilomatrixoma. Salivary gland tumors discussed are pleomorphic adenoma, mucoepidermoid carcinoma, adenoid cystic carcinoma and acinic cell carcinoma. Vascular anomalies are covered in another section.
Keywords: cholesteatoma, craniopharyngioma, encephalocele, teratomas, dermoid cysts, neuroblastoma, pilomatrixoma, thyroglossal duct cysts, salivary gland tumors
Potensi Pemanfaatan Atap Tribun Stadion Kapten I Wayan Dipta Gianyar sebagai PLTS Rooftop
Authors: Pradika Gallant , Giriantari Ida Ayu Dwi , Setiawan I Nyoman 
Published: January 2021

PLTS atap stadion Kapten I Wayan Dipta dirancang sebagai catu daya tambahan untuk memenuhi kebutuhan beban listrik stadion. Perancangan PLTS atap menggunakan 2 skenario. Skenario 1 memiliki kapasitas 83.300 Wp dengan modul surya sejumlah 238 unit dan inverter 2 unit menghasilkan energi 112.321 kWh/tahun dan akan menyuplai beban siang trafo 2 dan 3 stadion sebesar 35.470 kWh/tahun. Skenario 2 berkapasitas 156.800 Wp dengan modul surya sejumlah 448 unit dan 4 inverter menghasilkan energi sebesar 211,458 kWh/tahun dan akan menyuplai beban siang trafo 1 stadion sebesar 100.919 kWh/tahun. Biaya investasi skenario 1 sebesar Rp.1.178.760.000 dengan total pendapatan sampai tahun ke-25 sebesar Rp.1.090.635.084 sedangkan pada skenario 2 biaya investasi sebesar Rp.2.237.860.000 dan pendapatan sampai tahun ke-25 sebesar Rp.3.126.761.273 Pada analisis kelayakan ekonomi menggunakan metode NPV, PI, dan DPP hanya skenario 2 yang dapat dikatakan layak karena pada tahun ke-25 pendapatan lebih besar dari investasi, sedangkan pada skenario 1 pendapatan akan bernilai sama dengan investasi saat tahun ke-29. Hal ini berarti sistem pada skenario 1 tidak layak karena memiliki DPP lebih lama dibandingkan umur proyek.
Perancangan Penstock (Pipa Pesat) Untuk Pembangkit Listrik Tenaga Mikrohidro (PLTMH) di Banjar Dinas Mekarsari, Desa Panji, Kecamatan Sukasada, Kabupaten Buleleng
Authors: Widiana I Nengah , Giriantari Ida Ayu Dwi , Setiawan I Nyoman 
Published: January 2021

Sejak tahun 1980 banjar dinas Mekar Sari Desa Panji telah memiliki Pembangkit Listrik Tenaga Mikrohidro (PLTMH). PLTMH yang memiliki kapasitas pembangkitan 10kW ini mengalami berbagai permasalahan yang mengakibatkan operasinya yang tidak sesuai. Salah satu perbaikan yang diusulkan adalah perancangan penstock (pipa pesat) yang sesuai dengan PLTMH banjar dinas Mekar Sari. Perancangan penstock yang baru menghasilkan penstock dengan panjang 54 meter, diameter penstock 0,8272 meter dan tebal penstock sebesar 0,002 meter. Untuk kecepatan aliran pada penstock setelah di rancang baru adalah 2,7088 m/s, dan head bersih adalah 16 meter. Perbaikan ini dimaksudkan untuk memanfaatkan tinggi aliran sungai secara maksimal, sehingga kecepatan aliran dapat dihasilkan maksimal untuk selanjutnya digunakan memutar turbin.
Difference in the Faecal Elastase-1 Concentration between Resectable and Unresectable Pancreatic Cancer
Authors: M Abdul Rahman , Simadibrata Marcellus , Hasan Irsan , Suhendro Suhendro , Mudjadid E 
Published: January 2021

Background: In the pancreatic cancer can occur pancreatic exocrine insufficiency (PEI) that can be detected by measurement of fecal elastase-1 level. The aim of this study was to identify the proportion and the degree of PEI, proportion of steatorrhea in pancreatic cancer, the concentration difference of faecal elastase-1 between resectable and unresectable pancreatic cancer and mean concentration difference of faecal elastase-1 based on the stage of pancreatic cancer.Method: This was a cross-sectional study to determine the concentration difference of faecal elastase-1 between resectable and unresectable pancreatic cancer. This research was conducted at Cipto Mangunkusumo hospital, several network hospitals of Cipto Mangunkusumo hospital, and Wahidin Sudirohusodo Makasar hospital from November 2014 until May 2015. The statistical test used to assess differences in the levels of faecal elastase-1 between resectable and unresectable pancreatic cancer was Mann Whitney and Kruskal Wallis test was performed to assess the differences between the mean levels of faecal elastase 1 based on staging pancreatic cancer.Results: A total of 48 subjects with pancreatic cancer participated in this study, with resectable category was 19 (39.6%) subjects, and 29 (60.4%) subjects were unresectable. The proportion of patients with pancreatic cancer who experienced PEI was 75% (CI 95% 0.63 - 0.87) and the proportion of patients with pancreatic cancer who showed steatorrhea symptoms was 68.8% (CI 95% 0.557 - 0.819). There was no significant difference of faecal elastase-1 levels (P = 0.738) between the resectable and unresectable whereas the resectable group median value was 38.0 (15-500) μg/g and in unresectable group was 35.0 (15-500) μg /g. There was no significant difference (p = 0.767) in faecal elastase-1 levels based on the stage of pancreatic cancer with median (range) in stage IB 36 (15-100) pg/g, stage IIA 62 (15-500) pg/g, stage III 15 (15-500) μg/g, and stage IV 36 (15-500) μg/g.Conclusion: This study found a high proportion of PEI and steatorrhea in pancreatic cancer. There was no significant difference in faecal elastase-1 levels between the resectable and unresectable pancreatic cancer. There was no significant difference between mean levels of faecal elastase-1 based on the stage of pancreatic cancer.
A Case of Eosinophilic Gastritis with Gastric Bleeding
Authors: Sudrajat Dedy G , Maulahela Hasan 
Published: January 2021

Eosinophilic gastritis is a manifestation of gastrointestinal eosinophilic disease. The clinical symptoms are not specific, similar to other gastric disease complaints. The diagnostic aproach aside from clinical manifestations is also from endoscopic and histopathological features as the gold standard. Management includes drugs and diet. In some cases with strictures and perforations, endoscopic or even surgical intervention needed. Steroid-based therapy, especially topical preparations, is still the main choice. Other alternative therapies such as immunosuppression and targeted therapy showed good results in several case reports and small-scale studies. Further research to get a better management needed, considering the prevalence of this disease is elevated.
Wizja ponadlokalna... O Praktykach czytania Grażyny B. Tomaszewskiej
Authors: Waligóra Janusz 
Published: January 2021

The following article is a review of Grażyna Tomaszewska’s book entitled Praktyki czytania. Ponowoczesna interpretacja a szkoła (Gdańsk 2019). One of the major advantages of this publication is a contextual teaching strategy. Not only does it help to define the subject area of the text but also enables the reader to go beyond the presence and relate to the tradition, which tends to be a tough task in school reality. Thanks to an interesting mixture of the cultural texts, the localness (in the form of separateness and distinction) can mirror itself in a wider historical perspective. As a result, both the old and the contemporary show the quality of universality. In order to broaden the contexts, Tomaszewska also uses (post) modern interpretation theories (feminism, postcolonialism), which give the texts a fresh look and revise previous interpretations.
Being impressive theoretically-wise as it is (literary theory, literary science, didactics), Tomaszewska’s book still seems to have more powerful impact in practical aspects. Here, we can witness how interpretations of cultural texts are created, how they surprise and provoke. The author picks contemporary poems of Szymborska, Hartwig, Miłosz and Herbert along with Wiesław Myśliwski’s prose (Kamień na kamieniu) and Jacek Dehnel’s novel Matka Makryna. Recapturing of the tradition is presented through the books and heroes of Romanticism (including the mother Makryna), which, once again, proved to be a sense-making and life- giving movement that raises questions and brings meanings.
Scorpionism in east Minas Gerais: clinical and epidemiological aspects
Authors: De Matos Ione Maria , De Macedo Leandro Roberto , Ramiro Lívia Cristina , Alves Larissa Rocha Manhães , Pinto da Silva Jéssica Genoveva Boline Passarelli Capaz 
Published: January 2021

Objective: To report scorpion stings that occurred in eastern Minas Gerais. Methods: Data were collected retrospectively from medical records of patients admitted by scorpionism in Municipal Hospital. Statistical analyses were performed using the characterization of the total cases through descriptive statistics, which was presented in absolute and relative frequencies. The study was approved by the Research Ethics Committee. Results: There were 4,618 cases of scorpion stings, with the highest incidence from October to December. The males were more affected. The majority accidents were classified as mild occurrences. Local pain was reported in 98.04% of cases. The elapsed time between the accident and medical care varied mostly between 0-3 hours, the upper limbs were the most affected area. There was a total of 12 deaths predominantly in the 1–14 age group that presented systemic complications, with the cause of death being acute pulmonary edema and/or shock. Conclusion: The variables age, the elapsed time between the accident and medical care and the severity of symptoms played a pivotal role in death after poisoning by scorpion sting.
Dwa kościoły. Budownictwo kultowe w międzywojennych Niemczech jako przestrzeń modernistycznych eksperymentów
Authors: Makała Rafał 
Published: January 2021

The time between WW I and II was a period of intensive development of church architecture in Germany. In the new situation after the defeat in WW I on the wave of Christian renewal movements, the concept of the church as a building corresponding to its functions, as an object expressing the character of religion and the vision of a congregation as a community in modern society was re -formulated. The dynamically developing church architecture was an area of intense experiments (especially in the 1920s.), creating new forms, as well as devising new iconography by Rudolf Schwartz, Otto Bartning, or Dominikus Böhm. The paper draws attention to a certain community of the main antagonized Christian and Protestant denominations on the example of two buildings erected on the eastern periphery of the then Germany (from 1945 constituting the western part of Poland): the Catholic Church of St Anthony in Schneidemühl (now: Piła, Hans Herkommer, 1928–1930) and the Protestant Cross-Church in Stettin (now: Szczecin, Adolf Thesmacher, 1929–1931). The first was built in a small town as a representative seat of the Prelature, a branch of the Catholic Church in the Protestant region, near the then border with (revived again) Poland. The building is a continuation of an innovative and conservative concept realized by Herkommer at the Frauenfriedenskirche in Frankfurt am Main (1927–1929), and is a testimony to the search for forms expressing the rationalist aspirations for the renewal of the Catholic Church, however without abandoning the main principles of the Tradition. For this purpose, Herkommer applies ‘industrial’ forms used in the Bauhaus circle, creating a clearly avant-garde building: not only in the local context of a small border town of eastern Germany, but also in the Catholic tradition of sacred architecture. Hiring an avant-garde architect and using modernist forms was the decision of one man: Monsignor Maximilian Kaller, the leader of the Prelature. The Church of the Cross in Szczecin was raised in a luxurious district of a great Protestant city, so it was the parish church of the Protestant elite. Although built of brick and clearly referring to the tradition of the Gothic architecture of this region, the Church of the Cross also reveals its striving for the maximum reduction of forms and the use of the language of abstraction. When building a Protestant church, Thesmacher resorted to forms applied primarily in Catholic architecture, especially to the forms used by Herkommer. Thesmacher created a facility expressing attachment to the local tradition and manifesting the modernity of the Evangelical church in Pomerania. As a result, both churches are a testimony to functionalist aspirations, although, of course, the functions differed from those on which, for example, the founders of the Bauhaus were focused.