bluuebunny/crossref_metadata_2025_split huggingface dataset:
Prolonged Culturing of IPSC-derived Brain Endothelial-like Cells Is Associated With Quiescence, Downregulation of Glycolysis, and Resistance to Disruption by an Alzheimer’s Brain Milieu
Authors: Williams Lindsey M , Fujimoto Takashi , Weaver Riley R , Logsdon Aric F , Wilson Miranda L , Evitts Kira M , Young Jessica E , Banks William A , Erickson Michelle A 
Published: October 2021

Abstract
Background: Human induced pluripotent stem cell (hiPSC)-derived brain endothelial-like cells (iBECs) are a robust, scalable, and translatable model of the human blood-brain barrier (BBB). Prior works have shown that high transendothelial electrical resistance (TEER) persists in iBECs for at least two weeks, emphasizing the utility of the model for longer term studies. However, most studies evaluate iBECs within the first few days of subculture, and little is known about their proliferative state, which could influence their functions. In this study, we characterized iBEC proliferative state in relation to key BBB properties at early (2 days) and late (9 days) post-subculture time points. Methods: hiPSCs were differentiated into iBECs using fully defined, serum-free medium. The proportion of proliferating cells was determined by BrdU assays. We evaluated TEER, expression of glycolysis enzymes and tight and adherens junction proteins (TJP and AJP), and glucose transporter-1 (GLUT1) function by immunoblotting, immunofluorescence, and quantifying radiolabeled tracer permeabilities. We also compared barrier disruption in response to TNF-α and conditioned medium (CM) from hiPSC-derived neurons harboring the Alzheimer’s disease (AD)-causing Swedish mutation (APPSwe/+).Results: A significant decline in iBEC proliferation over time in culture was accompanied by adoption of a more quiescent endothelial metabolic state, indicated by downregulation of glycolysis-related proteins and upregulation GLUT1. Interestingly, upregulation of GLUT1 was associated with reduced glucose transport rates in more quiescent iBECs. We also found significant decreases in claudin-5 (CLDN5) and vascular endothelial-cadherin (VE-Cad) and a trend toward a decrease in platelet endothelial cell adhesion molecule-1 (PECAM-1), whereas zona occludens-1 (ZO-1) increased and occludin (OCLN) remained unchanged. Despite differences in TJP and AJP expression, there was no difference in mean TEER on day 2 vs. day 9. TNF-α induced disruption irrespective of iBEC proliferative state. Conversely, APPSwe/+ CM disrupted only proliferating iBEC monolayers. Conclusion: iBECs can be used to study responses to disease-relevant stimuli in proliferating vs. more quiescent endothelial cell states, which may provide insight into BBB vulnerabilities in contexts of development, brain injury, and neurodegenerative disease.
Rapid Deployment and Application Exploration of the Mobile Shelter Laboratories Under T He Outbreak of COVID-19 Epidemic
Authors: Luan Yi , Ding Rui , Gu Wenshen , Zhang Xiaofan , Chen Xinliang , Deng Yawen , Fang Qimei , Wei Jie , Duan Chaohui 
Published: October 2021

Abstract
Since the end of 2019, the COVID-19 epidemic has swept the world. With the widespread spread of the COVID-19 and the continuous emergence of mutated strains, the situation for the prevention and control of the COVID-19 epidemic remains severe. On May 21, 2021, Guangzhou City, Guangdong Province, notified the discovery of a new locally confirmed case. Guangzhou became the first city in mainland China to compete with the delta mutant strain. As a local hospital with strong nucleic acid detection capabilities, Sun Yat-sen University Sun Yat-sen Memorial Hospital took the lead in launching the construction and deployment of the Mobile Shelter Laboratories and large-scale screening work in Foshan and Zhanjiang, Guangdong Province. Through summarizing "practical" experience, observation and comparison data analysis, we use real data to verify a feasible solution for rapid expansion of detection capabilities in a short period of time. We hope that these experiences will have certain reference value for other countries or regions, especially the underdeveloped areas of medical and health care.
Pan-cancer Analyses Reveal Regulation and Clinical Outcome Association of the PCLAF in Human Tumors
Authors: Liu Xiaowei , Cheng Cheng , Cai Yuanxia , Gu Yaoyao , Wu Yangkun , Chen Kai , Wu Zhixiang 
Published: October 2021

Abstract
Background: PCLAF, also known as KIAA0101, is overexpressed among some human malignancies and performs distinct biological functions. However, the specific mechanism of PCLAF in cancer remains unclear. Therefore, this study aims to analyze the role of PCLAF in human tumors.Methods: First, we used TCGA (Cancer Genome Atlas) and GEO (Gene expression Synthesis) data sets to evaluate the expression level of PCLAF in 33 tumors. Secondly, we used GEPIA2 and Kaplan-Meier plotter for survival prognosis analysis. In addition, we use cBioPortal web to analyze the genetic changes of PCLAF and perform immune infiltration analysis. At the same time, we also conducted enrichment analysis of PCLAF-related genes. Finally, we selected the hepatocellular carcinoma (HCC) cell line to verify the function of PCALF.Results: PCLAF is highly expressed in most cancers, and there is a significant correlation between the expression of PCLAF and the prognosis of tumor patients. The expression of PCLAF is positively correlated with Act CD4 (Activated CD4 T cells) and Th2 (Type 2 T helper cells), suggesting that PCLAF may play a particular role in tumor immune infiltration. In addition, the functional mechanism of PCLAF also involves the mitotic cell cycle process, cell division, DNA replication. Conclusions: Our first pan-cancer study provides a relatively comprehensive understanding of the carcinogenic effects of PCLAF in different tumors, which provides a solid foundation for studying the role of PCLAF in tumorigenesis and development.
Converting waste PET plastics into automobile fuel and antifreeze components
Authors: Zhao Chen , Gao Zhiwen , Chen Shuang , Ma Bing , Tian Jingqing 
Published: October 2021

Abstract
With the aim to solve the serious problem of white plastic pollution, we report herein a H2-free low-cost Cu/SiO2 catalyzed process to convert polyethylene terephthalate (PET) into p-xylene (PX) and ethylene glycol (EG) in one pot with methanol as both the solvent and hydrogen source at 210 °C. Kinetic and in-situ Fourier-transform infrared spectroscopy (FTIR) studies demonstrated that the degradation of PET into dimethyl terephthalate (DMT) in methanol involved methanol dehydrogenation and ester hydrodeoxygenation reactions. When preparing the copper silicate precursor using the hydrothermal method with ammonia, NaCl was introduced to interact with the surface hydroxyl groups of SiO2, thereby forming a dense, granular copper silicate with a high Cu+/Cu0 ratio, which improved the methanol dehydrogenation and DMT hydrodeoxygenation activities. This novel H2-free process uses a low-cost Cu-based catalyst, allowing us to produce gasoline and antifreeze components from waste PET plastic in one pot, which provides a feasible solution to the plastic pollution problem in islands.
Data Agreement Analysis And Correction of Comparative Geomagnetic Vector Observations
Authors: He Zhaobo , Hu Xingxing , Teng Yuntian , Zhang Xiuxia , Shen Xiaoyu 
Published: October 2021

Abstract
Geomagnetism, similar to other areas of geophysics, is an observation-based science. Data agreement between comparative geomagnetic vector observations is one of the most important evaluation criteria for high-quality geomagnetic data. The main influencing factors affecting the agreement between comparative observational data are the attitude angle, scale factor, long-term time drift, and temperature. In this paper, we propose a method based on a genetic algorithm and linear regression to correct for these effects and use the distribution pattern of points in Bland–Altman plots with a 95% confidence interval length to qualitatively and quantitatively evaluate the agreement between the comparative observational data. In Bland–Altman plots with better agreement, that is, with the corrected data, more than 95% of the points are distributed within the 95% confidence interval and there is no obvious pattern in the distribution of the points. Meanwhile, the length of 95% confidence interval decreased significantly after the correction. The method presented here has positive effects on the vector instrumentation detection, enhancing the robustness of comparative observatory observations and reducing errors in judgments of the size and arrival time of large magnetic disturbances or rapid magnetic variations.
Global epidemiology of Gaucher disease: an updated systematic review and meta-analysis
Authors: Wang Meimei , Li Fengqin , Zhang Jing , Lu Cheng , Meng Yan , Ding Yingxue , Kong Weijing 
Published: October 2021

Abstract
Background

Gaucher disease, an autosomal recessive lysosomal storage disorder, is characterized by progressive lysosomal storage of glucocerebroside in macrophages predominantly in bone, bone marrow, liver, and spleen. Meta-analysis of global Gaucher disease epidemiology was not available prior to this study.
Methods

To provide a systematic review and meta-analysis of birth prevalence and prevalence of Gaucher disease in multiple countries. MEDLINE and EMBASE databases were searched for original research articles on the epidemiology of Gaucher disease from inception until July 21, 2021. Meta-analysis, adopting a random effects logistic model, was performed to estimate birth prevalence and prevalence of Gaucher disease.
Results

Eighteen studies that were screened out of 1874 records were included for data extraction. The studies that fulfilled the criteria for inclusion involved 15 areas/countries. The global birth prevalence of Gaucher disease was 1.5 cases (95% CI: 1.0-2.0) per 100,000 live births. The global prevalence of Gaucher disease was 0.9 cases (95% CI: 0.7-1.1) per 100,000 inhabitants.
Conclusions

To our knowledge, this is the first comprehensive systematic review that presented quantitative data by evaluating global epidemiology of Gaucher disease. Quantitative data of global epidemiology of Gaucher disease could be the fundamental to evaluate the global efforts that improve many factors, including diagnostic technology and data collection, which affect global epidemiology of Gaucher disease.
Assessing The Suitability of New Film Laminates For Sustainable Insect Eradication By Modified Atmosphere In Museums
Authors: Elkhial Manar , Elhadidi Nesrin 
Published: October 2021

Abstract
The increasing demand for applying modified inert atmosphere (MIA) systems for insect eradication in museums has led to the desire for lower-cost consumable materials, particularly laminated plastic films. An ultra-low oxygen-permeable laminate is required for creating successful MIA systems to keep the oxygen concentration lower than 0.3%, which is commercially available but at a high cost. The wide use of local laminated films for food preservation makes them a perfect target for testing and improvement for MIA applications. However, the lack of laboratory oxygen permeability test methods to gauge the potential of local laminates for inclusion in MIA applications distracts attention from looking at them as alternatives and encourages the expense on extremely expensive imported ones. Therefore, the present work investigates the potential of employing two laminates (one local and one imported) to create a successful leak-proof MIA system. A laboratory easy-to-use test method was developed to assess the oxygen-gas retention property of each laminate by measuring its oxygen permeability and consequently oxygen transmission rate (OTR). The test method is a sealed static diffusion chamber separated in the middle by a known area (cm2) of the test laminate to be tested. The test relies on measuring the concentration of oxygen in either sides of the laminate membrane within the sealed system and monitors the change over time to assess the OTR of the laminate. The specifications and design of the test chamber are adapted from the ASTM Designation: E2945 − 14, to meet the facilities of a typical artefact fumigation laboratory. The test is undertaken at standard MIA conditions (temperature of 25°C, relative humidity of 45%, and target oxygen concentration of 0.3). Results indicated that the new method is useful for an unlimited number of tests of an unlimited number of laminates. The conducted tests proved that the local laminate normally used for food packaging has superior advantages over the long-used imported ones.
Measurement of Mitral Valve Area By Direct Three Dimensional Planimetry Compared To Multiplanar Reconstruction In Patients With Rheumatic Mitral Stenosis
Authors: Tabrizi Mehrnoush Toufan , Azad Haniyeh Faraji , Khezerlouy-Aghdam Naser , Sakha Hanieh 
Published: October 2021

Abstract
Background: Mitral valve area (MVA) measurement by three-dimensional transesophageal echocardiography (3D-TEE) has a crucial role in the evaluation of mitral stenosis (MS) severity. Three-dimensional direct (3D-direct) planimetry has been proposed as a new technique to measure mitral valve area. This study aimed to compare the 3D-direct mitral valve planimetry to conventional three-dimensional multiplanar reconstruction (3D-MPR) in severe mitral stenosis (MS) using 3D-TEE.Methods: 149 patients with severe MS who were referred for percutaneous transmitral commissurotomy (PTMC), prospectively recruited. All patients underwent 2D transthoracic echocardiography (2D-TTE) and 3D-TEE in a single session before PTMC. During 2D-TTE planimetry, pressure half time (PHT), and proximal isovelocity surface area (PISA) were applied to measure the MVA. Transmitral mean pressure gradient (MPG) was measured. During 3D-TEE, MVA planimetry was carried out with both 3D-direct and 3D-MPR methods. 3D-direct was applied from both atrial and ventricular views. The consistency of MVA measurements with 3D-direct, 3D-MPR, and 2D-TTE methods was statistically investigated.Results: Our sample consisted of 109 (73.2%) women and 40 (26.8%) men. The mean age was 51.75 ± 9.81 years. The agreement between 3D-direct and 3D-MPR planimetry was significant and moderate (0.99 ± 0.29 cm2 vs. 1.12 ± 0.26 cm2, Intraclass Correlation = 0.716, p value =0.001).The accuracy of the 3D-direct method reduced significantly compared to the MPR method at MVA > 1.5 cm2. The maximum difference between two methods was observed in cases with MVAs larger than 1.5 cm2. MVA measured with the 3D-MPR method was significantly correlated with a 2D-TTE method, with a moderate agreement (Intraclass Correlation = 0.644, p value = 0.001). Also, 2D-TTE and 3D-direct TEE techniques yielded significantly consistent measurements of the MVA (1.06 ± 0.026 cm2 vs. 0.99 ± 0.29 cm2, Intraclass Correlation = 0.787, p value = 0.001); however, with a slight overestimation of the MVA by the former with a net difference of 0.06 ± 0.013 cm2. Mitral valve pressure gradient (MPG) had no significant correlation with planimetry results. A significant inverse correlation was seen between the MVA and pulmonary arterial systolic pressure.Conclusion: 3D-direct planimetry has an acceptable agreement with 3D-MPR planimetry at MVA less than 1.5 cm2, but their correlation decreases significantly at MVA above 1.5 cm2. 3D-direct planimetry underestimates MVA compared to 3D-MPR, especially at MVA above 1.5 cm2. The 2D-TTE planimetry has generally acceptable accuracy, but its correlation to the 3D-TEE methods is significantly reduced in cases with moderate to severe MS (i.e. MVA> 1.0cm2).
Reversible Slow Desensitization of the Acid-Sensing Ion Channel (ASIC) 1a
Authors: Komarova Margarita S , Bukharev Andrey R , Potapieva Natalia N , Tikhonov Denis B 
Published: October 2021

Abstract
Among the proton-activated channels of the ASIC family, ASIC1a exhibits a specific tachyphylaxis phenomenon in the form of a progressive decrease in the response amplitude during a series of activations. This process is well known, but its mechanism is poorly understood. Here, we demonstrated a partial reversibility of this effect by long-term whole-cell recording of CHO cells transfected with rASIC1a cDNA. Long but infrequent acidifications provided the same recovery time course as short acidifications of the same frequency. Steady-state desensitization is not related to the slow desensitization and attenuates the development of the slow desensitization. Consequently, we found that drugs, which facilitate ASIC1a activation (e.g., amitriptyline), cause an enhancement of slow desensitization, while inhibition of ASIC1a by 9-aminoacridine attenuates the slow desensitization. In summary, for influences of vastly different origin, including increase of calcium concentration, different pH conditions, and action of modulating drugs, we found a correlation between the effect on response amplitude and on development of slow desensitization. Thus, our results prove that a slow desensitization requires the open ion-permeable state.
First-In-Human in Vivo Imaging and Quantification of Monoacylglycerol Lipase in The Brain: A PET Study With 18F-T-401
Authors: Takahata Keisuke , Seki Chie , Kimura Yasuyuki , Kubota Manabu , Ichise Masanori , Sano Yasunori , Yamamoto Yasuharu , Tagai Kenji , Shimada Hitoshi , Kitamura Soichiro , Matsuoka Kiwamu , Endo Hironobu , Shinotoh Hitoshi , Kawamura Kazunori , Zhang Ming-Rong , Takado Yuhei , Higuchi Makoto 
Published: October 2021

Abstract
Purpose: Monoacylglycerol lipase (MAGL) regulates cannabinoid neurotransmission and the pro-inflammatory arachidonic acid pathway by degrading endocannabinoids. MAGL inhibitors may accordingly act as cannabinoid-potentiating and anti-inflammatory agents. Although MAGL dysfunction has been implicated in neuropsychiatric disorders, it has never been visualized in vivo in human brain. The primary objective of the current study was to visualize MAGL in the human brain using the novel PET ligand 18F-T-401. Methods: Seven healthy males underwent 120-min dynamic 18F-T-401-PET scans with arterial blood sampling. Six subjects also underwent a second PET scan with 18F-T-401 within 2 weeks of the first scan. For quantification of MAGL in the human brain, kinetic analyses using one- and two-tissue compartment models (1TCM and 2TCM, respectively), along with multilinear analysis (MA1) and Logan graphical analysis were performed. Time-stability and test-retest reproducibility of 18F-T-401-PET were also evaluated.Results: 18F-T-401 showed rapid uptake and gradual washout from the brain. Logan graphical analysis showed linearity in all subjects, indicating reversible radioligand kinetics. Using a metabolite-corrected arterial input function, MA1 estimated regional total distribution volume (VT) values by best identifiability. VT values were highest in the cerebral cortex, moderate in the thalamus and putamen, and lowest in white matter and the brainstem, which was in agreement with regional MAGL expression in the human brain. Time-stability analysis showed that MA1 estimated VT values with a minimal bias even using truncated 60-min scan data. Test-retest reliability was also excellent with the use of MA1. Conclusions: Here, we provide the first demonstration of in vivo visualization of MAGL in the human brain. 18F-T-401 showed excellent test-retest reliability, reversible kinetics, and stable estimation of VT values consistent with known regional MAGL expressions. PET with 18F-T-401-PET is promising tool for measurement of central MAGL.
Virulence and Genetic Characterization of Six Baculovirus Strains Isolated From Different Populations of Spodoptera Frugiperda (Lepidoptera: Noctuidae)
Authors: Zanella-Saenz Ingrid , Herniou Elisabeth A. , Ibarra Jorge E. , Rincón-Castro Ma.Cristina Del , Huerta-Arredondo Ilse Alejandra 
Published: October 2021

Abstract
Fall armyworm (FAW), Spodoptera frugiperda (Smith, 1797), is a polyphagous, voracious, and economically important agricultural pest. Biological control of FAW is a strategy that must be further explored. This study evaluated six baculovirus strains isolated from infected FAW larvae from Mexico, Argentina, Honduras, and the United States. Five alphabaculoviruses (SfNPV-An2, SfNPV-Arg, SfNPV-Fx, SfNPV-Ho and SfNPV-Sin) and one betabaculovirus (SfGV-RV), were tested against FAW larvae, showing a wide diversity of virulence levels among strains when their estimated LC50s were compared, being SfNPVArg, SfNPV-Ho and SfNPV-Fx more virulent than SfNPV-An 2 , SfNPV-Sin and SfGV-RV. To determine any virulence difference in vitro studies of these isolates, Sf9 cell cultures were used. Interestingly, only ODVs from four of the test SfNPV strains showed infectivity on Sf9 cell cultures, and some differences in virulence were observed. Genomic restriction analyses and partial sequences of lef-8, lef-9 , and polh/granulin genes showed little variability among alphabaculoviruses, both, among them and with previously reported sequences. However, sequences from SfGV-RV were closer to previously reported sequences from the SfGVVG008 strain than the SfGV-Arg and SfGV-VG014 strains. The great difference in the in vivo virulence was not correlated with great similarity among the isolates. The characterization of these six baculoviruses isolates offers the basis for exploring their potential as biological control agents against S. frugiperda, as well the initial studies on their specific infection mechanisms, evolution, and ecology.
Optimum Application of Hybrid Data Driven Models With Two Step Verification Method at Mangla Watershed, Pakistan
Authors: Tayyab Muhammad , Xiaohua Dong , Sibtain Muhammad , Ahmad Ijaz , Zahra Aqeela , Azam Muhammad Imran 
Published: October 2021

Abstract
In this study, 03 ensemble and decomposition methods (DMs) i.e., empirical mode decomposition (EMD), ensemble empirical mode decomposition (EEMD) and improved complete ensemble empirical mode decomposition with additive noise (ICEEMDAN) were coupled artificial intelligence and machine learning based method AI-ML, i.e., multilayer perceptron (MLP), support vector regression (SVR) to develop 06 fundamental hybrid models to predict streamflow with one-month lead time. Developed models in this study were categorized into runoff models (RMs) and rainfall-runoff models (RRMs). Results indicated that (i) among standalone models (SMs), support vector regression (SVR) performs better than multilayer perceptron (MLP), (ii) decomposition methods (DMs) have ability to improve the accuracy rate of the standalone models (SMs) and, (iii) rainfall runoff models (RRMs) have shown great accuracy throughout the investigation as compared to runoff models (RMs). To compare model performances flow-hydrographs (FHG) were generated, 05 performance evaluation criteria (PEC) were used to quantify the model precision. Two step verification method i.e., extreme value analysis (EVA) and least value analysis (LVA) approaches were proposed to verify the performances. Among all developed hybrid models (HMs), i.e., EMD- (MLP, SVR), EEMD- (MLP, SVR) and ICEEMDAN- (MLP, SVR), rainfall-runoff ICEEMDAN-(SVR) model was selected as optimal model with MAE (59.56), RMSE (91.82), R (0.97) MAPE (8.75), and NSEC (0.97) for Mangla watershed, Pakistan.