bluuebunny/crossref_metadata_2025_split huggingface dataset:
EVALUATION OF POTENTIAL ANTIMICROBIAL ACTIVITY OF SYNTHETIC FLAVONOIDS
Authors: Bhixavatimath Prabhulingayya S , Maniyar Yasmeen , Naikawadi Akram , Vijayakumar D  
Published: February 2021

Introduction: In recent times, most of the currently available antimicrobial agents have developed resistance. Extensive pharmacological activities including bactericidal and bacteriostatic nature of flavonoids, made them as priority agents in this aspect of research study. Synthetic flavonoids such as hydroxy thiophen derivatives were considered to evaluate for antimicrobial activity in this study.
Objective: The present study involves the analysis for antimicrobial activity of thiophen substituted synthetic flavonoids.
Methods: Claisen-Schmidt method of condensation fallowed by oxidative cyclization reactions from substituted hydroxyacetophenone with aromatic aldehydes were used to synthesize the various analogues of flavonoid compounds. Then these compounds after their FTIR, 1H NMR, MS spectral characterization and elemental analysis, were screened for in vitro antibacterial and antifungal activity by using disc diffusion method followed by determining their respective zone of inhibitions.
Results: All the synthesized test flavonoid compounds exhibited the good antibacterial and antifungal spectrum activity over B. subtilis, S. aureus, E. coli and P. aeurugenosa bacteria and Candida albicans and Aspergillus niger fungal microbes. However compounds such as F1, F2 and F4 showed moderately significant antibacterial activity against P. aerugenosa organism than the other test compounds and the same F1 and F2 test compounds exhibited significant antifungal activity at100µg concentration.
Conclusion: The present study demonstrated that the novel thiophen substituted flavonoids (F1, F2, F3 and F4 ) found to have promising antimicrobial and antifungal activity which needs to be confirmed by in vivo studies.
Pengelolaan Pembelajaran Dalam Proses Pengembangan Sosial Emosional Standar Pendidikan Anak Usia Dini
Authors: Siti Rosmayati  , Arman Maulana  , Sofyan Sauri  , Ujang Cepi Barlian  
Published: March 2021

In connection with descriptive data analysis, this research will describe from the results of documentation and field observations from the analysis of learning management in the process of social emotional development of PAUD Az-Zahra Islamic Preschool Cicalengka, Bandung Regency The results of the analysis documentation carried out by researchers on the management of learning in the process of social emotional development aged 4-5 years of 17 children at PAUD Az-Zahra Islamic Preschool Cicalengka, Bandung Regency. There are several indicators in learning management 1) Planning 2) Implementation of learning, namely activities before entering class, preliminary activities, core activities, eating and resting activities, closing activities 3) Evaluation, namely Assessment, Reporting and follow-up. So it can be concluded that the management of learning in the process of social emotional development of PAUD Az-Zahra Islamic Preschool Cicalengka Bandung Regency has been done by the teacher well according to the indicator although there are still two indicators that have not been implemented, the success of the teacher can be seen from the level of achievement of emotional social development a good boy.
APLIKASI HOME SOLAR SYSTEM SEBAGAI PENERANGAN UNTUK TPQ AL-MURTADHO DI KOTA MALANG
Authors: Asrori Asrori , Harijono Agus , Faizin Akhmad , Dani Agus , Kriswitono Kriswitono 
Published: February 2021

Lokasi TPQ-Al Murtadho terletak di RW 01, Wonokoyo, Kedungkandang, Kota Malang. Bangunan semi permanen ini didirikan secara swadaya oleh warga setempat. Selain berfungsi sebagai taman pendidikan Al-Quran (TPQ), bangunan ini juga dimanfaatkan untuk tempat musyawarah warga. Sistem penerangan yang murah dan mudah sangat relevan untuk diterapkan pada bangunan tersebut. Oleh karena itu dalam PkM ini dilakukan pelatihan singkat dan pemasangan sistem penerangan bertenaga surya (home solar system). Kegiatan yang dilakukan bersama pemuda karang taruna ini berlangsung selama dua hari. Pelatihan berupa presentasi mengenai listrik tenagasurya, yang dilanjutkan dengan perakitan dan pemasangan instalasi panel surya di TPQ Al-Murtadho. Hasil PkM adalah paket listrik tenaga surya yang terdiri dari : 1 unit panel surya; 1 unit solar charger controller; 1 unit baterai; 3 lampu LED dan peralatan aksesoris. Panel surya yang digunakan adalah 100 WP (Watt Peak) artinya daya maksimum yang mampu dibangkitkan oleh panel surya dalam 1 jam adalah 100 Watt. Jika sinar matahari dalam kondisi normal (cerah) diasumsikan 5 jam, maka panel surya mampu menghasilkan listrik sebesar 500 Wattjam. Sehingga dengan energi tersebut akan mampu untuk menyalakan lampu dengan daya 9 Watt pada 3 titik selama 9-10 jam.
PERAN ETIKA BISNIS ISLAM DALAM MEMBANGUN KINERJA LEMBAGA KEUANGAN SYARIAH
Authors: Efendi Bahtiar , Trihudiyatmanto M. 
Published: February 2021

Tujuan dari Penelitian ini adalah menganalisis seberapa besar pengaruh Locus of Control terhadap kinerja usaha. Variabel yang diteliti yaitu Variabel Independen terdiri dari Variabel Locus of control dan Variabel dependen adalah kinerja usaha dengan etika bisnis islam sebagai variabel moderasi.
Data penelitian diperoleh dari karyawan BMT Ummat Sejahtera Banjarnegara Penarikan sampel dari populasi menggunakan metode sensus sampling. Data dikumpulkan dengan menggunakan metode survey, yaitu kuesioner diantar dan diambil langsung diobyek penelitian.
Analisis data menggunakan uji Structural equation modeling (SEM) dengan pengecekan antara variabel independen, variabel moderating dan variabel dependen. Dari penelitian yang telah dilakukan didapatkan hasil bahwa Locus of control tidak berpengaruh terhadap kinerja karyawan di BMT Ummat Sejahtera Banjarnegara sedangkan Etika bisnis islami berpengaruh terhadap kinerja karyawan di BMT Ummat Sejahtera Banjarnegara. Etika bisnis islami memoderasi pengaruh Locus of control terhadap kinerja karyawan di BMT Ummat Sejahtera Banjarnegara.
Clinical spectrum, outcome and management of immune thrombocytopenia associated with myelodysplastic syndromes and chronic myelomonocytic leukemia
Authors: Jachiet Vincent , Moulis Guillaume , Hadjadj Jérome , Seguier Julie , Laribi Kamel , Schleinitz Nicolas , Vey Norbert , Sacre Karim , Godeau Bertrand , Beyne-Rauzy Odile , Bouvet Romain , Broner Jonathan , Brun Natacha , Comont Thibault , Gaudin Clément , Lambotte Olivier , Le Clech Lenaïg , Peterlin Pierre , Roy-Peaud Frédérique , Salvado Clémentine , Versini Mathilde , Isnard Françoise , Kahn Jean Emmanuel , Gobert Delphine , Adès Lionel , Fenaux Pierre , Fain Olivier , Mekinian Arsène 
Published: February 2021

Myelodysplastic syndromes (MDS) and chronic myelomonocytic leukemia (CMML) are associated with systemic inflammatory or autoimmune diseases in 10-20 % of cases. Among them, immune thrombocytopenia (ITP) has been reported but large studies assessing this association are missing. Whether such patients have a particular phenotype and require particular management is unclear.
This study analyzes the clinical spectrum, outcome and therapeutic management of patients with ITP associated with MDS or CMML, in comparison (i) to patients with primary ITP without MDS/CMML and (ii) to patients with MDS/CMML without ITP.
Forty-one MDS/CMML-associated ITP patients were included, with chronic ITP in 26 (63%) patients, low-risk myelodysplasia in 30 (73%) patients and CMML in 24 (59%) patients. An associated autoimmune disease was noted in 10 (24%) patients. In comparison to primary ITP patients, MDS/CMML-associated ITP patients had a higher occurrence of severe bleeding despite similar platelet counts at diagnosis. First-line treatment consisted of glucocorticoids (98%) and intravenous immunoglobulin (IVIg) (56%). Response achievement with IVIg was more frequent in primary ITP than in MDS/CMML-associated ITP patients. Response rates to second-line therapies were not statistically different between primary ITP and MDS/CMMLassociated ITP patients. Ten percent (n=4) of patients with MDS/CMML-associated ITP had multirefractory ITP versus none in primary ITP controls. After a median follow-up of 60 months, there was no difference in overall survival between MDS/CMML-associated ITP and primary ITP patients. Leukemia-free-survival was significantly better in MDS/CMMLassociated ITP patients than in MDS/CMML without ITP MDS/CMML-associated ITP have a particular outcome with more severe bleeding and multirefractory profile than primary ITP, similar response profile to primary ITP therapy except for IVIg, and less progression toward acute myeloid leukemia than MDS/CMML without ITP.
Use of Metabolomic Approaches in Analysis of Medicinal Plants and Phytopreparations (Review)
Authors: Orlova A. A. , Strugar Jo. , Shtark O. Yu. , Zhukov V. A. , Luzhanin V. G. , Povydysh M. N. 
Published: February 2021

Introduction. The purpose of the analytical review is to summarize the data of modern scientific literature on the directions and possibilities of using the approaches of metabolomics in the analysis of medicinal plants, plant raw materials and herbal drugs.Text. Analysis of literature data showed that metabolomic approaches have great potential in the field of quality control of multicomponent phytopreparations and biologically active additives, detection of falsifications of rare and expensive plant materials, chemosystematics of medicinal plants, study of the mechanisms of action and toxicity of medicinal plants, etc.Conclusion. Metabolic analysis can become an effective analytical platform both for phytochemical research of plant raw materials and for regular activities to control the quality of plant material and phytopreparations.
Clinical Significance of a Novel Tumor Progression-Associated Immune Signature in Colorectal Adenocarcinoma
Authors: Mao Rui , Yang Fan , Wang Zheng , Xu Chenxin , Liu Qian , Liu Yanjun , Zhang Tongtong 
Published: February 2021

BackgroundSome colorectal adenocarcinoma (CRC) patients are susceptible to recurrence, and they rapidly progress to advanced cancer stages and have a poor prognosis. There is an urgent need for efficient screening criteria to identify patients who tend to relapse in order to treat them earlier and more systematically.MethodsWe identified two groups of patients with significantly different outcomes by unsupervised cluster analysis of GSE39582 based on 101 significantly differentially expressed immune genes. To develop an accurate and specific signature based on immune-related genes to predict the recurrence of CRC, a multivariate Cox risk regression model was constructed with a training cohort composed of 519 CRC samples. The model was then validated using 129, 292, and 446 samples in the real-time quantitative reverse transcription PCR (qRT-PCR), test, and validation cohorts, respectively.ResultsThis classification system can also be used to predict the prognosis in clinical subgroups and patients with different mutation states. Four independent datasets, including qRT-PCR and The Cancer Genome Atlas (TCGA), demonstrated that they can also be used to accurately predict the overall survival of CRC patients. Further analysis suggested that high-risk patients were characterized by worse effects of chemotherapy and immunotherapy, as well as lower immune scores. Ultimately, the signature was identified as an independent prognostic factor.ConclusionThe signature can accurately predict recurrence and overall survival in patients with CRC and may serve as a powerful prognostic tool to further optimize cancer immunotherapy.
Development of a Rapid and Fully Automated Multiplex Real-Time PCR Assay for Identification and Differentiation of Vibrio cholerae and Vibrio parahaemolyticus on the BD MAX Platform
Authors: Li Zhenpeng , Guan Hongxia , Wang Wei , Gao He , Feng Weihong , Li Jie , Diao Baowei , Zhao Hongqun , Kan Biao , Zhang Jingyun 
Published: February 2021

Vibrio cholerae and Vibrio parahaemolyticus are common diarrheal pathogens of great public health concern. A multiplex TaqMan-based real-time PCR assay was developed on the BD MAX platform; this assay can simultaneously detect and differentiate V. cholerae and V. parahaemolyticus directly from human fecal specimens. The assay includes two reactions. One reaction, BDM-VC, targets the gene ompW, the cholera toxin (CT) coding gene ctxA, the O1 serogroup specific gene rfbN, and the O139 serogroup specific gene wbfR of V. cholerae. The other, BDM-VP, targets the gene toxR and the toxin coding genes tdh and trh of V. parahaemolyticus. In addition, each reaction contains a sample process control. When evaluated with spiked stool samples, the detection limit of the BD MAX assay was 195–780 CFU/ml for V. cholerae and 46–184 CFU/ml for V. parahaemolyticus, and the amplification efficiency of all genes was between 95 and 115%. The assay showed 100% analytical specificity, using 63 isolates. The BD MAX assay was evaluated for its performance compared with conventional real-time PCR after automated DNA extraction steps, using 164 retrospective stool samples. The overall percent agreement between the BD MAX assay and real-time PCR was ≥ 98.8%; the positive percent agreement was 85.7% for ompW, 100% for toxR/tdh, and lower (66.7%) for trh because of a false negative. This is the first report to evaluate the usage of the BD MAX open system in detection and differentiation of V. cholerae and V. parahaemolyticus directly from human samples.
Baseline Volumetric T2 Relaxation Time Histogram Analysis: Can It Be Used to Predict the Response to Intravenous Methylprednisolone Therapy in Patients With Thyroid-Associated Ophthalmopathy?
Authors: Liu Ping , Luo Ban , Chen Lang , Wang Qiu-Xia , Yuan Gang , Jiang Gui-hua , Zhang Jing 
Published: February 2021

ObjectivePrediction of therapy response to intravenous methylprednisolone pulses (ivMP) is crucial for thyroid-associated ophthalmopathy (TAO). Image histograms may offer sensitive imaging biomarkers for therapy effect prediction. This study aimed to investigate whether pretherapeutic, multiparametric T2 relaxation time(T2RT) histogram features of extraocular muscles (EOMs) can be used to predict therapy response.Materials and MethodsForty-five active and moderate-severe TAO patients, who were treated with standard ivMP and underwent orbital MRI before therapy, were retrospectively included in this study. The patients were divided into responsive (n = 24, 48 eyes) and unresponsive group(n = 21, 42 eyes) according to clinical evaluation. Baseline clinical features of patients and histogram-derived T2RT parameters of the EOMs were analyzed and compared. Logistic regression model was conducted to determine independent predictors, and a histogram features nomogram was formulated for personalized prediction.ResultsResponsive group displayed lower values for 5th, 10th percentiles (P &lt; 0.050, respectively), and higher values for 75th, 90th, and 95th percentiles, skewness, entropy, and inhomogeneity (P &lt; 0.050, respectively) than unresponsive group. Multivariate logistic regression analysis showed that 95th percentile of &gt;88.1 [odds ratio (OR) = 12.078; 95% confidence interval (CI) = 3.98–36.655, p &lt; 0.001], skewness of &gt;0.31 (OR = 3.935; 95% CI = 2.28–6.788, p &lt; 0.001) and entropy of &gt;3.41 (OR = 4.375; 95% CI = 2.604–7.351, p &lt; 0.001) were independent predictors for favorable response. The nomogram integration of three independent predictors demonstrated optimal predictive efficiency, with a C-index of 0.792.ConclusionsPre-treatment volumetric T2RT histogram features of EOMs could function to predict the response to ivMP in patients with TAO. The nomogram based on histogram features facilitates the selection of patients who will derive maximal benefit from ivMP.
Differential Effect of Metabolic Health and Obesity on Incident Heart Failure: A Nationwide Population-Based Cohort Study
Authors: Kim Hwi Seung , Lee Jiwoo , Cho Yun Kyung , Park Joong-Yeol , Lee Woo Je , Kim Ye-Jee , Jung Chang Hee 
Published: February 2021

BackgroundMetabolically healthy obese (MHO) individuals and their association with cardiometabolic diseases have remained controversial. We aimed to explore the risk of incident heart failure (HF) based on the baseline metabolic health and obesity status as well as their transition over 2 years.MethodsThe Korean National Health Insurance Service-National Health Screening Cohort data of 514,886 participants were analyzed. Obesity was defined as BMI ≥25 kg/m2 according to the Korean Centers for Disease Control and Prevention. The metabolic health and obesity status were evaluated at baseline and after two years. Study participants were followed to either the date of newly diagnosed HF or the last follow-up visit, whichever occurred first.ResultsThe MHO group comprised 9.1% of the entire population and presented a better baseline metabolic profile than the metabolically unhealthy non-obese (MUNO) and metabolicavlly unhealthy obese (MUO) groups. During the median 71.3 months of follow-up, HF developed in 5,406 (1.5%) participants. The adjusted hazard ratios [HRs (95% CI)] of HF at baseline compared with the metabolically healthy non-obese (MHNO) group were 1.29 [1.20–1.39], 1.37 [1.22–1.53], and 1.63 [1.50–1.76] for MUNO, MHO, and MUO groups, respectively. With the stable MHNO group as reference, transition into metabolically unhealthy status (MUNO and MUO) increased the risk of HF, regardless of the baseline status. Subjects who were obese at both baseline and follow-up showed an increased risk of HF, regardless of their metabolic health status.ConclusionsMetabolic health and obesity status and their transition can predict the risk of incident HF. Losing metabolic health in baseline non-obese and obese individuals and remaining obese in baseline obese individuals showed a significantly increased risk of incident HF. Maintaining good metabolic health and a lean body may prevent the development of HF.
Case Report: Reversible Neurotoxicity and a Clinical Response Induced by BCMA-Directed Chimeric Antigen Receptor T Cells Against Multiple Myeloma With Central Nervous System Involvement
Authors: Zhang Ying , Zhang Changfeng , Zhou Jin , Zhang Jingren , Chen Xiaochen , Chen Jia , Wang Pu , Sun Xiuli , Lou Xiaoyan , Qi Wei , Kang Liqing , Yu Lei , Wu Depei , Li Caixia 
Published: February 2021

Isolated central nervous system involvement in multiple myeloma (CNS-MM) is rare and carries extremely poor prognosis. Chimeric antigen receptor T cell therapy (CART) targeting B-cell maturation antigen (BCMA) is demonstrated as a promising strategy in MM treatment, but the clinical safety and efficacy of BCMA-CART against isolated CNS-MM remain elusive. Here we report on a 56-year-old male with refractory isolated CNS-MM who received autologous BCMA-CART therapy and developed grade 4 neurological complications. Cerebrospinal fluid (CSF) analyses showed significant expansion of CART cells and a substantially elevated interleukin-6 (IL-6) level. Intravenous methylprednisolone was administered and the symptoms resolved gradually. Unexpectedly, the level of IL-6 in the CSF was maintained for another 3 days even after the relief of the neurological symptoms. A partial response was achieved and sustained for 5.5 months. This is the first report describing a patient with isolated CNS-MM treated using BCMA-CART therapy. The results demonstrated that BCMA-CART cells administered intravenously trafficked into the CSF, eradicated tumor cells, and induced severe but reversible neurological adverse events. This single-patient report suggests that BCMA-CART therapy can be considered as an alternative option for isolated CNS-MM.Clinical Trial RegistrationClinicalTrials.gov, identifier NCT03196414.
Clonorchis sinensis-Derived Protein Attenuates Inflammation and New Bone Formation in Ankylosing Spondylitis
Authors: Lee Yu Jeong , Kim Moon-Ju , Jo Sungsin , Jin So-Hee , Park Pu-Reum , Park Kijeong , Song Ho-Chun , Kim Jahae , Kim Ji-Young , Shim Seung Cheol , Kim Tae-Hwan , Hong Sung-Jong , Kang Hyundeok , Kim Tae-Jong , Won Eun Jeong 
Published: February 2021

Helminth infections and their components have been shown to have the potential to modulate and attenuate immune responses. The objective of this study was to evaluate the potential protective effects of Clonorchis sinensis-derived protein (CSp) on ankylosing spondylitis (AS). Cytotoxicity of CSp at different doses was assessed by MTS and flow cytometry before performing experiments. Peripheral blood mononuclear cells (PBMCs) and synovial fluid mononuclear cells (SFMCs) were obtained from AS patients. Inflammatory cytokine-producing cells were analyzed using flow cytometry. The levels of INF-γ, IL-17A, TNF-α, and IL-6 were measured by enzyme-linked immunosorbent assay (ELISA). SKG mice were treated with CSp or vehicles. Inflammation and new bone formation were evaluated using immunohistochemistry, positron emission tomography (PET), and micro-computed tomography (CT). Treatment with CSp resulted in no reduced cell viability of PBMCs or SFMCs until 24 h. In experiments culturing PBMCs and SFMCs, the frequencies of IFN-γ and IL-17A producing cells were significantly reduced after CSp treatment. In the SKG mouse model, CSp treatment significantly suppressed arthritis, enthesitis, and enteritis. Micro-CT analysis of hind paw revealed reduced new bone formation in CSp-treated mice than in vehicle-treated mice. We provide the first evidence demonstrating that CSp can ameliorate clinical signs and cytokine derangements in AS. In addition, such CSp treatment could reduce the new bone formation of AS.
The Impact of Oral-Gut Inflammation in Cerebral Palsy
Authors: Ferreira Ana Cristina Fernandes Maria , Eveloff Ryan J. , Freire Marcelo , Santos Maria Teresa Botti Rodrigues 
Published: February 2021

Background: Oral-gut inflammation has an impact on overall health, placing subjects at risk to acquire chronic conditions and infections. Due to neuromotor disturbances, and medication intake, cerebral palsy (CP) subjects present intestinal constipation, impacting their quality of life (QOL). We aimed to investigate how oral inflammatory levels predicted gut phenotypes and response to therapy.Methods: A total of 93 subjects aging from 5 to 17 years were included in the study, and assigned into one of the 4 groups: CP with constipation (G1, n = 30), CP without constipation (G2, n = 33), and controls without CP with constipation (G3, n = 07) and without CP and without constipation (G4, n = 23). In addition to characterizing subjects' clinical demographics, medication intake, disease severity levels, salivary cytokine levels [TNF-α, interleukin (IL)-1β, IL-6, IL-8, IL-10], and Caregiver Priorities and Child Health Index of Life with Disabilities (CPCHILD). Statistical significance was evaluated by Shapiro-Wilks, Student's T-Test, ANOVA, and ANCOVA analysis.Results: Salivary proinflammatory cytokines were highly correlated with the severe form of gut constipation in G1 (P &lt; 0.001), and out of all cytokines IL-1β levels demonstrated highest correlation with all gut constipation (P &lt; 0.05). A significant relationship was found between the type of medication, in which subjects taking Gamma-Aminobutyric Acid (GABA) and GABA+ (GABA in association with other medication) were more likely to be constipated than the other groups (P &lt; 0.01). Cleary salivary inflammatory levels and gut constipation were correlated, and impacted QOL of CP subjects. G1 presented a lower QOL mean score of CPCHILD (49.0 ± 13.1) compared to G2 (71.5 ± 16.7), when compared to G3 (88.9 ± 7.5), and G4 (95.5 ± 5.0) (P &lt; 0.01). We accounted for gingival bleeding as a cofounder of oral inflammation, and here were no differences among groups regarding gender (P = 0.332) and age (P = 0.292).Conclusions: Collectively, the results suggest that saliva inflammatory levels were linked to gut constipation, and that the clinical impact of medications that controlled gut was reliably monitored via oral cytokine levels, providing reliable and non-invasive information in precision diagnostics.