bluuebunny/crossref_metadata_2025_split huggingface dataset:
Differences in cerebral small vessel disease magnetic resonance imaging markers between lacunar stroke and non–Lobar intracerebral hemorrhage
Authors: Wiegertjes Kim , Jansen Michelle G , Jolink Wilmar MT , Duering Marco , Koemans Emma A , Schreuder Floris HBM , Tuladhar Anil M , Wermer Marieke JH , Klijn Catharina JM , Leeuw Frank-Erik de 
Published: August 2021

Introduction It is unclear why cerebral small vessel disease (SVD) leads to lacunar stroke in some and to non–lobar intracerebral hemorrhage (ICH) in others. We investigated differences in MRI markers of SVD in patients with lacunar stroke or non–lobar ICH. Patients and methods We included patients from two prospective cohort studies with either lacunar stroke (RUN DMC) or non–lobar ICH (FETCH). Differences in SVD markers (white matter hyperintensities [WMH], lacunes, cerebral microbleeds [CMB]) between groups were investigated with univariable tests; multivariable logistic regression analysis, adjusted for age, sex, and vascular risk factors; spatial correlation analysis and voxel–wise lesion symptom mapping. Results We included 82 patients with lacunar stroke (median age 63, IQR 57–72) and 54 with non-lobar ICH (66, 59–75). WMH volumes and distribution were not different between groups. Lacunes were more frequent in patients with a lacunar stroke (44% vs. 17%, adjusted odds ratio [aOR] 5.69, 95% CI [1.66–22.75]) compared to patients with a non–lobar ICH. CMB were more frequent in patients with a non–lobar ICH (71% vs. 23%, aOR for lacunar stroke vs non–lobar ICH 0.08 95% CI [0.02–0.26]), and more often located in non–lobar regions compared to CMB in lacunar stroke. Discussion Although we obserd different types of MRI markers of SVD within the same patient, ischemic markers of SVD were more frequent in the ischemic type of lacunar stroke, and hemorrhagic markers were more prevalent in the hemorrhagic phenotype of non-lobar ICH. Conclusion There are differences between MRI markers of SVD between patients with a lacunar stroke and those with a non-lobar ICH.
Levetiracetam in management of bilateral trigeminal neuralgia due to large glomus tumor case report
Authors: Hsieh Billie , Benkli Barlas , Ansoanuur George N , Bonfante-Mejia Eliana E , Smart Shaun O 
Published: August 2021

Background: Trigeminal neuralgia can be classical or idiopathic. While trigeminal neuralgia (TN) due to space-occupying lesions is atypical, such lesions rarely cause severe TN secondary to trigeminal nerve irritation. Mass effect from these lesions has been shown to correlate with symptom burden, due to direct or indirect compressive effects. A tethering effect, provoked by an abnormal root-stretching force, theoretically plays a role in trigeminal nerve hyperexcitability. Case: The likely etiology in this case presentation is a large glomus tumor invading the middle and posterior cranial fossa. Glomus tumors are uncommon benign tumors of the head and neck derived from neural crest cells. Even more strikingly, a large glomus tumor causes bilateral TN due to direct compression on one side and indirect compression on the contralateral side. Conclusion: Although the gold standard in TN management is carbamazepine, other anti-epileptic drugs (AEDs) have been used in the treatment of patients unable to take carbamazepine. A few studies suggest levetiracetam alleviates central and neuropathic pain, supporting the hypothesis that it may be effective in management of TN.
A novel method for predicting cell abundance based on single-cell RNA-seq data
Authors: Peng Jiajie , Han Lu , Shang Xuequn 
Published: August 2021

Abstract
Background
It is important to understand the composition of cell type and its proportion in intact tissues, as changes in certain cell types are the underlying cause of disease in humans. Although compositions of cell type and ratios can be obtained by single-cell sequencing, single-cell sequencing is currently expensive and cannot be applied in clinical studies involving a large number of subjects. Therefore, it is useful to apply the bulk RNA-Seq dataset and the single-cell RNA dataset to deconvolute and obtain the cell type composition in the tissue.

Results
By analyzing the existing cell population prediction methods, we found that most of the existing methods need the cell-type-specific gene expression profile as the input of the signature matrix. However, in real applications, it is not always possible to find an available signature matrix. To solve this problem, we proposed a novel method, named DCap, to predict cell abundance. DCap is a deconvolution method based on non-negative least squares. DCap considers the weight resulting from measurement noise of bulk RNA-seq and calculation error of single-cell RNA-seq data, during the calculation process of non-negative least squares and performs the weighted iterative calculation based on least squares. By weighting the bulk tissue gene expression matrix and single-cell gene expression matrix, DCap minimizes the measurement error of bulk RNA-Seq and also reduces errors resulting from differences in the number of expressed genes in the same type of cells in different samples. Evaluation test shows that DCap performs better in cell type abundance prediction than existing methods.

Conclusion
DCap solves the deconvolution problem using weighted non-negative least squares to predict cell type abundance in tissues. DCap has better prediction results and does not need to prepare a signature matrix that gives the cell-type-specific gene expression profile in advance. By using DCap, we can better study the changes in cell proportion in diseased tissues and provide more information on the follow-up treatment of diseases.
Implementation of pharmaceutical services in Brazilian primary health care: a cross-sectional study
Authors: Pereira Nathália Cano , Luiza Vera Lucia , Campos Mônica Rodrigues , Chaves Luisa Arueira 
Published: August 2021

AbstractBackgroundIn the Brazilian public health system, primary health care (PHC) is provided by the municipalities and is considered the entry level of the Unified Health System (SUS). Governmental pharmaceutical services (PharmSes) are part of the SUS, including PHC, and are the most significant way in which patients access medicine and services. Considering the diversity of the country, the municipalities have the autonomy to decide how PharmSes are implemented. Even though policies and procedures should be implemented as expected by policy makers and experts, municipality characteristics may interfere with implementation fidelity. Therefore, this study evaluated the degree to which the PharmSes in PHC were delivered as intended in Brazilian municipalities.MethodsWe analysed data from a secondary database originating from a cross-sectional nationwide study carried out by the Ministry of Health and the World Bank from 2013 to 2015. Data on 465 municipalities and the Federal District were collected from 4939 governmental PharmSes. A rating system comprising 43 indicators was developed and applied to the dataset to obtain the implementation degree (ID) of each PharmSe. Additionally, the IDs of the two PharmSes dimensions and the nine components were measured.ResultsOverall, the ID of the PharmSes in Brazilian PHC was evaluated as critical. The ID was critical in 81% of the municipalities (n = 369), incipient in 14% (n = 65) and unsatisfactory in 4.8% (n = 22). Regarding the PharmSes dimensions, the ‘medicine management’ (MM) ID was considered critical (Mean = 46%), while the ‘care management’ (CM) ID was incipient (Mean = 22%). In terms of the PharmSes components, the highest ID was achieved by ‘forecasting’ (58%). In contrast, ‘continuing education and counselling’ showed the lowest figure (ID = 11%) in the whole sample, followed by ‘information and communication’ and ‘teamwork’.ConclusionsThe degree to which PharmSes were implemented was critical (ID< 50%). This analysis demonstrated that PharmSes were implemented with low fidelity, which may be related to the low availability of medicine in PHC. Although the care management component requires more attention, considering their incipient ID, all components must be reviewed. Municipalities must increase their investment in PharmSes implementation in order to maximize the benefits of these services and guarantee the essential right of access to medicine.
Pseudomonas otitidis bacteraemia in a patient with COPD and recurrent pneumonia: case report and literature review
Authors: Caixinha Adriana L. , Valsamidis Alexandros N. , Chen Ming , Lindberg Mats 
Published: August 2021

Abstract
Background
Pseudomonas otitidis is a novel species of Pseudomonas bacteria that has been isolated from patients with otic infections.

Case presentation
In this report, we describe a case of a 59-year-old known with moderate chronic obstructive pulmonary disease with bronchiectasis and recurrent pneumonia where blood cultures revealed the growth of P. otitidis.

Conclusions
This case describes the first report of bacteraemia to P. otitidis and raises questions regarding the misdiagnosis and underestimation of the incidence of infections caused by this novel pathogen.
Factors associated with early 14-day unplanned hospital readmission: a matched case–control study
Authors: Lo Yu-Tai , Chang Chia-Ming , Chen Mei-Hua , Hu Fang-Wen , Lu Feng-Hwa 
Published: August 2021

Abstract
Background/Purpose
Early unplanned hospital readmissions are burdensome health care events and indicate low care quality. Identifying at-risk patients enables timely intervention. This study identified predictors for 14-day unplanned readmission.

Methods
We conducted a retrospective, matched, case–control study between September 1, 2018, and August 31, 2019, in an 1193-bed university hospital. Adult patients aged ≥ 20 years and readmitted for the same or related diagnosis within 14 days of discharge after initial admission (index admission) were included as cases. Cases were 1:1 matched for the disease-related group at index admission, age, and discharge date to controls. Variables were extracted from the hospital’s electronic health records.

Results
In total, 300 cases and 300 controls were analyzed. Six factors were independently associated with unplanned readmission within 14 days: previous admissions within 6 months (OR = 3.09; 95 % CI = 1.79–5.34, p < 0.001), number of diagnoses in the past year (OR = 1.07; 95 % CI = 1.01–1.13, p = 0.019), Malnutrition Universal Screening Tool score (OR = 1.46; 95 % CI = 1.04–2.05, p = 0.03), systolic blood pressure (OR = 0.98; 95 % CI = 0.97–0.99, p = 0.01) and ear temperature within 24 h before discharge (OR = 2.49; 95 % CI = 1.34–4.64, p = 0.004), and discharge with a nasogastric tube (OR = 0.13; 95 % CI = 0.03–0.60, p = 0.009).

Conclusions
Factors presented at admission (frequent prior hospitalizations, multimorbidity, and malnutrition) along with factors presented at discharge (clinical instability and the absence of a nasogastric tube) were associated with increased risk of early 14-day unplanned readmission.
Development and evaluation of recombinase polymerase amplification combined with lateral flow dipstick assays for co-detection of epizootic haemorrhagic disease virus and the Palyam serogroup virus
Authors: Li Zhuo-ran , Yang Zhen-xing , Li Zhan-hong , Gao Xiang , Hu Zhong-yan , Yang Heng , Liao De-fang 
Published: August 2021

Abstract
Background
Epizootic haemorrhagic disease virus (EHDV) and the Palyam serogroup viruses (PALV) have led to significant economic losses associated with livestock production globally. A rapid, sensitive and specific method for the detection of EHDV and PALV is critical for virus detection, monitoring, and successful control and elimination of related diseases.

Results
In the present study, a recombinase polymerase amplification combined with lateral flow dipstick (RPA-LFD) assay for the co-detection of genome segment 1 (Seg-1) of EHDV and PALV was developed and evaluated. The analytical sensitivities of the established RPA-LFD assay in the detection of EHDV and PALV were 7.1 copies/µL and 6.8 copies/µL, respectively. No cross-reaction with other members of the genus Orbivirus, including African horse sickness virus, bluetongue virus, Guangxi orbivirus, Tibet orbivirus and Yunnan orbivirus was observed. The established RPA-LFD assay accurately detected 39 EHDV strains belonging to 5 serotypes and 29 PALV strains belonging to 3 serotypes. The trace back results of quantitative real-time polymerase chain reaction (qRT-PCR) and the established RPA-LFD assay on sentinel cattle were consistent. The coincidence rates of qRT-PCR and the established RPA-LFD assay in 56 blood samples from which EHDV or PALV had been isolated and 96 blood samples collected from cattle farms were more than 94.8 %. The results demonstrated that the established RPR-LFD assay is specific, sensitive and reliable, and could be applied in early clinical diagnosis of EHDV and PALV.

Conclusions
This study highlights the development and application of the RPA-LFD assay in the co-detection of EHDV and PALV for the first time. The assay could be used as a potential optional rapid, reliable, sensitive and low-cost method for field diagnosis of EHDV and PALV.
Absent from DNA and protein: genomic characterization of nullomers and nullpeptides across functional categories and evolution
Authors: Georgakopoulos-Soares Ilias , Yizhar-Barnea Ofer , Mouratidis Ioannis , Hemberg Martin , Ahituv Nadav 
Published: August 2021

Abstract
Nullomers and nullpeptides are short DNA or amino acid sequences that are absent from a genome or proteome, respectively. One potential cause for their absence could be their having a detrimental impact on an organism.

Results
Here, we identify all possible nullomers and nullpeptides in the genomes and proteomes of thirty eukaryotes and demonstrate that a significant proportion of these sequences are under negative selection. We also identify nullomers that are unique to specific functional categories: coding sequences, exons, introns, 5′UTR, 3′UTR, promoters, and show that coding sequence and promoter nullomers are most likely to be selected against. By analyzing all protein sequences across the tree of life, we further identify 36,081 peptides up to six amino acids in length that do not exist in any known organism, termed primes. We next characterize all possible single base pair mutations that can lead to the appearance of a nullomer in the human genome, observing a significantly higher number of mutations than expected by chance for specific nullomer sequences in transposable elements, likely due to their suppression. We also annotate nullomers that appear due to naturally occurring variants and show that a subset of them can be used to distinguish between different human populations. Analysis of nullomers and nullpeptides across vertebrate evolution shows they can also be used as phylogenetic classifiers.

Conclusions
We provide a catalog of nullomers and nullpeptides in distinct functional categories, develop methods to systematically study them, and highlight the use of variability in these sequences in other analyses
Restoration of keratinocytic phenotypes in autonomous trisomy-rescued cells
Authors: Tanuma-Takahashi Akiko , Inoue Momoko , Kajiwara Kazuhiro , Takagi Ryo , Yamaguchi Ayumi , Samura Osamu , Akutsu Hidenori , Sago Haruhiko , Kiyono Tohru , Okamoto Aikou , Umezawa Akihiro 
Published: August 2021

Abstract
Background
An extra copy of chromosome 21 in humans can alter cellular phenotypes as well as immune and metabolic systems. Down syndrome is associated with many health-related problems and age-related disorders including dermatological abnormalities. However, few studies have focused on the impact of trisomy 21 (T21) on epidermal stem cells and progenitor cell dysfunction. Here, we investigated the differences in keratinocytic characteristics between Down syndrome and euploid cells by differentiating cells from trisomy 21-induced pluripotent stem cells (T21-iPSCs) and autonomous rescued disomy 21-iPSCs (D21-iPSCs).

Methods
Our protocol for keratinocytic differentiation of T21-iPSCs and D21-iPSCs was employed. For propagation of T21- and D21-iPSC-derived keratinocytes and cell sheet formation, the culture medium supplemented with Rho kinase inhibitor on mouse feeder cells was introduced as growth rate decreased. Before passaging, selection of a keratinocytic population with differential dispase reactivity was performed. Three-dimensional (3D) air-liquid interface was performed in order to evaluate the ability of iPSC-derived keratinocytes to differentiate and form stratified squamous epithelium.

Results
Trisomy-rescued disomy 21-iPSCs were capable of epidermal differentiation and expressed keratinocytic markers such as KRT14 and TP63 upon differentiation compared to trisomy 21-iPSCs. The lifespan of iPSC-derived keratinocytes could successfully be extended on mouse feeder cells in media containing Rho kinase inhibitor, to more than 34 population doublings over a period of 160 days. Dispase-based purification of disomy iPSC-derived keratinocytes contributed epidermal sheet formation. The trisomy-rescued disomy 21-iPSC-derived keratinocytes with an expanded lifespan generated 3D skin in combination with a dermal fibroblast component.

Conclusions
Keratinocytes derived from autonomous trisomy-rescued iPSC have the ability of stratification for manufacturing 3D skin with restoration of keratinocytic functions.
Does a policy that requires adherence to a regular primary care physician improve the actual adherence of patients?
Authors: Golan-Cohen A. , Blumberg G. , Merzon E. , Kitai E. , Fogelman Y. , Shipotovsky A. , Vinker S. 
Published: August 2021

Abstract
Background
Continuity of care by the same personal physician is a key factor in an effective and efficient health care system. Studies that support the association between high adherence and better outcomes were done in settings where allocation to the same physician was a long-term policy.

Objectives
To evaluate the influence that changing organizational policy from the free choice of a primary care physician to a mandatory continuity of care by the same physician has on adherence to a personal physician.

Methods
A cross-sectional study based on electronic databases; comparison of adherence and demographic characteristics (sex, age, and socio-economic status) of 208,286 Leumit enrollees who met the inclusion criteria, according to change in the adherence to a personal physician. To evaluate adherence, we used the Usual Provider of Care (UPC) index, which measures the number of visits made to the personal doctor out of the total primary care physician visits over the same period. The patients were divided into groups according to their UPC level.

Results
The data shows that 54.5% of the patients were high adherers even before the organizational change; these rates are similar to those published by various organizations worldwide, years after mandating continuity of care by the same physician.
In the year following the intervention, only 34.5% of the patients changed the level of their adherence group. Of these, 64% made a shift to a higher adherence group.
Before the intervention, the high adherers were older (mean age 57.8 vs. 49.3 years in the low adherers group) and from a higher SES (mean SES status 9.32 vs. 8.71). After the intervention, a higher proportion of older patients and patients from a higher SES changed their adherence to a higher group.
Sex distribution was similar over all the adherence level groups and did not change after the intervention.

Conclusions and policy implications
A policy change that encouraged adherence to an allocated primary care physician managed to improve adherence only in specific groups.
Health organizations need to examine the potential for change and the groups they want to influence and direct their investment wisely.

Trial registration
retrospectively registered.
Delayed skin reaction after mRNA-1273 vaccine against SARS-CoV-2: a rare clinical reaction
Authors: Hoff Norman-Philipp , Freise Noemi F. , Schmidt Albrecht G. , Firouzi-Memarpuri Parnian , Reifenberger Julia , Luedde Tom , Bölke Edwin , Meller Stephan , Homey Bernhard , Feldt Torsten , Jensen Björn Erik Ole , Keitel Verena , Schmidt Livia , Maas Kitti , Haussmann Jan , Tamaskovics Balint , Budach Wilfried , Fischer Johannes C. , Buhren Bettina Alexandra , Knoefel Wolfram Trudo , Schneider Marion , Gerber Peter Arne , Pedoto Alessia , Häussinger Dieter , Grebe Olaf , van Griensven Martijn , Braun Stephan A. , Salzmann Stefan , Rezazadeh Amir , Matuschek Christiane 
Published: August 2021

Abstract
Background
The coronavirus disease 2019 (COVID‐19) is associated with a wide clinical spectrum of skin manifestations, including urticarial, vesicular, vasculitic and chilblain‐like lesions. Recently, delayed skin reactions have been reported in 1% individuals following mRNA vaccination against SARS-CoV-2. The exact pathophysiology and the risk factors still remain unclear.

Patients and methods
6821 employees and patients were vaccinated at our institutions between February and June 2021. Every patient received two doses of the mRNA-1273 vaccine in our hospitals, and reported back in case of any side effects which were collected in our hospital managed database.

Results
Eleven of 6821 vaccinated patients (0.16%) developed delayed skin reactions after either the first or second dose of the mRNA-1273 vaccine against SARS-CoV-2. Eight of 11 patients (73%) developed a rash after the first dose, while in 3/11 (27%), the rash occurred after the second dose. More females (9/11) were affected. Four of 11 patients required antihistamines, with two needing additional topical steroids. All the cutaneous manifestations resolved within 14 days. None of the skin reactions after the first dose of the vaccine prevented the administration of the second dose. There were no long-term cutaneous sequelae in any of the affected individuals.

Conclusion
Our data suggests that skin reactions after the use of mRNA-1273 vaccine against SARS-CoV-2 are possible, but rare. Further studies need to be done to understand the pathophysiology of these lesions.
Simulation of Permeation of Saturated Cement Paste Based on a New Meso-scale Pore Network Model
Authors: Zhou Yong , Yang Yuxuan , Gyawali Bigya , Zhang Weiping 
Published: August 2021

AbstractThis paper presents the simulation of the permeation of saturated cement paste based on a novel pore network model. First, a 2D hydration model of cement particles was developed by extending the work of Zheng et al. 2005 to provide the background for the network construction. Secondly, the establishment of the pore network model and simulation of permeation of saturated cement paste were carried out. The irregular pores between any two hydrated cement particles were linearized with clear distances as the diameters of pores. The straight tubular pores were interconnected with one another to form the network model. During this process, the weighted Voronoi diagram was employed to operate on the graphical expression of the hydrated cement particles. Water permeation in saturated cement paste was simulated to verify the pore network model. Finally, the factors including water–cement ratio, reaction temperature, reaction time and cement particle size that would influence water permeation were numerically investigated.