bluuebunny/crossref_metadata_2025_split huggingface dataset:
165 Effects of Digestible Lysine Level on Growth Performance and Economics of Grow-finish Pigs
Authors: Becker Larissa L , Scholtz Emily E , DeRouchey Joel M , Tokach Mike D , Woodworth Jason C , Goodband Robert D , Gebhardt Jordan T , De Jong Jon A , Wu Fangzhou , Gourley Kiah M , Neill Casey R 
Published: May 2021

Abstract
A total of 2,124 barrows and gilts (PIC 1050′DNA 600, initially 48.9 kg) were used in a 32-d study to determine the optimal dietary standardized ileal digestibility (SID) Lys level in a commercial setting. Pigs were randomly allotted to 1 of 5 dietary treatments with 24 to 27 pigs/pen and 16 replications/treatment. Similar number of barrows and gilts were placed in each pen. Diets were fed over 3 phases (48.9 to 58.6, 58.6 to 70.9, and 70.9 to 80.8 kg respectively). Dietary treatments were corn-soybean meal-based and contained 10 (phase 1 and 2) or 5% (phase 3) distillers dried grains with solubles. Diets were formulated to 85, 95, 103, 110, or 120% of the current Pig Improvement Company (PIC, Hendersonville, TN) SID Lys gilt recommendations with phase 1 SID Lys levels of 0.90, 1.01, 1.09, 1.17 and 1.27%, phase 2 levels of 0.79, 0.87, 0.94, 1.03, and 1.10%, and phase 3 levels of 0.71, 0.78, 0.85, 0.92, and 0.99%, respectively. Dose response curves were evaluated using linear (LM), quadratic polynomial (QP), broken-line linear (BLL), and broken-line quadratic (BLQ) models. For each response variable, the best-fitting model was selected using the Bayesian information criterion. Overall (d 0 to 32), increasing SID Lys increased (linear, P&lt; 0.001) BW, ADG, G:F, Lys intake/d, and Lys intake/kg of gain. Modeling margin over feed cost (MOFC), BLL and QP estimated the requirement at 105.8% and 113.7% respectively. In summary, while growth increased linearly up to 120% of the PIC current feeding level, the optimal MOFC was 106% to 114% depending on the model used.
169 The Effect of Standardized Ileal Digestible isoleucine:lysine in Diets Containing 20% Dried Distillers Grains with Solubles on Finishing Pig Performance and Carcass Characteristics
Authors: Clizer David A , Samuel Ryan S , Cline Paul M 
Published: May 2021

Abstract
Previous research suggests an increase of the isoleucine (Ile) requirement for late finishing pigs. Diets containing dried distillers grains with solubles (DDGS) provide excess leucine which can lead to changes in available Ile due to the antagonistic relationship of branched chain amino acids. Therefore, the Ile requirement in finishing diets containing DDGS deserve to be re-evaluated. A finishing study was conducted to determine the standardized ileal digestible (SID) Ile:Lysine (Lys) requirement in diets containing 20% DDGS. Pigs (n = 2,268; 82.3 ± 0.39 kgs) were used in a 56-d trial (14 replicates per treatment). Pens were assigned to one of six dietary treatments balancing for previous treatment. Treatments consisted of a corn-soybean meal diet (CS) or diets containing 20% DDGS with a SID Ile:lys ratio of 55, 60, 65, 70, or 75%. Data was analyzed as a randomized complete block, pair-wise comparisons and single degree of freedom orthogonal polynomials (DDGS diets only) were used to evaluate treatment responses, and pen was the experimental unit. Increasing the SID Ile:Lys did not impact cumulative performance of pigs fed diets containing 20% DDGS (P &gt; 0.175). Pigs fed CS had improved (P &lt; 0.010) ADFI compared to pigs fed SID Ile:Lys of 65 and 75% and tended (P = 0.084) to have greater ADG than 55 and 75% Ile:Lys treatments. Feed efficiency did not differ due to treatment (P = 0.427). Increasing the SID Ile:Lys to 65% in 20% DDGS diets decreased back fat and increased loin depth (quadratic; P &lt; 0.029) and tended to increase percent lean (quadratic; P = 0.076), but did not alter hot carcass weight (P &gt; 0.428). Pigs fed CS diets had greater hot carcass weights (P &lt; 0.031) compared to pigs fed DDGS diets except for pigs fed 60% Ile:Lys diets. These data suggest a SID Ile:Lys of 60% is required to maximize growth performance and SID Ile:Lys of 65% increases carcass traits in diets containing 20% DDGS.
171 Effects of Dietary Valine, Isoleucine, and Tryptophan Supplementations to Diets Containing Excess Leucine on Nitrogen Balance of Growing Pigs
Authors: Kwon Woongbi Bi , Soto Jose A , Stein Hans H 
Published: May 2021

Abstract
An experiment was conducted to test the hypothesis that increasing concentrations of dietary Val, Ile, or Trp alone or in combination will alleviate negative effects of excess dietary Leu on N balance of growing pigs. Seventy-two barrows (initial body weight: 33.9 ± 2.6 kg) were housed in metabolism crates and randomly assigned to 1 of 8 diets and 3 blocks with 3 pigs per diet in each block in a 12-d experiment. Treatments were arranged in a 2 × 2 × 2 factorial with the main effects of L-Val (0 or 0.1%), L-Ile (0 or 0.1%), and L-Trp (0 or 0.05%) that were added to a basal diet containing corn and a high-protein corn product (48% crude protein; 5.9% Leu). The basal diet contained 1.00 % standardized ileal digestible (SID) Lys and 171% SID Leu:Lys. Data were analyzed using the PROC MIXED of SAS with concentrations of Val, Ile, and Trp, and all interactions as main effects and replicate as a random effect. No 3-way interactions were observed (Table 1). Results indicated that fecal N output increased if Ile was added to diets without added Val, but that was not the case if Val was added (interaction, P &lt; 0.05). Addition of Ile to diets reduced N retention, but N retention increased with Trp addition to diets without Val addition, but not if Trp was added to diets with added Val (interaction, P &lt; 0.05). The biological value of protein increased if Trp was added to diets without addition of Ile, but if Ile was added, Trp addition did not increase the biological value of protein (interaction, P &lt; 0.05). In conclusion, adding Ile to a diet with excess Leu reduced N retention, but if Trp was added alone or in combination with Ile or Val, N retention increased.
199 Effect of the Pelleting Process on Diet Formulations with Varying Levels of Crystalline Amino Acids and Reducing Sugars on Digestibility in Growing Pigs
Authors: Dunmire Kara M , Lopez Diego A , Fiehler Chance J , Zhang Yiqin , Jones Cassandra K , Li Yonghui , Woodworth Jason C , Goodband Robert D , Tokach Mike D , Stark Charles R , Stein Hans H , Paulk Chad B 
Published: May 2021

Abstract
The objective of this study was to determine effects of pelleting on the standardized ileal digestibility (SID) of amino acids (AA) in diets with or without increased concentrations of free AA and reducing sugars (RS). Eight individually housed, ileal cannulated barrows (initially 69.2 kg) were allotted to a replicated 8×8 Latin square with 8 diets and eight 7-d periods with ileal digesta collected on d 6 and 7. Treatments were arranged in a 2×2×2 factorial with main effects of diet form (mash vs. pellet), crystalline AA (low vs. high), or reducing sugars (low vs. high) provided by dried distillers grains with solubles and bakery meal. Diets were pelleted to achieve a hot pellet temperature of 85 to 88°C. Data were analyzed as a completely randomized Latin square using the GLIMMIX procedure of SAS. A feed form×RS interaction (P &lt; 0.026) for SID of tryptophan was observed. Feeding pelleted low RS diets improved SID of tryptophan compared with mash high and low RS diets, and pelleted high RS diets. For main effects of feed form, the SID of total AA, CP, and indispensable AA increased (P &lt; 0.042) in pigs fed pelleted diets compared with mash diets. For main effects of crystalline AA, pigs fed high crystalline AA had increased (P = 0.007) SID of tryptophan and decreased (P = 0.050) SID of histidine compared with those fed low crystalline AA diets. For main effects of RS diets, pigs fed high RS diets had decreased (P &lt; 0.05) SID of total AA, CP and indispensable AA. In conclusion, pelleting diets with increased crystalline AA or RS did not affect the improvement in AA digestibility from pelleting. Pelleting diets improved AA digestibility. Diets formulated with high crystalline AA had increased SID of tryptophan. Formulating diets with high RS resulted in decreased AA digestibility compared with corn-soybean meal-based diets.
201 Impact of Water Flow Rate on Finishing Pig Performance
Authors: Miller Hannah E , Perez-Palencia Jorge Y , Levesque Crystal L , Thaler Robert C 
Published: May 2021

Abstract
A survey of South Dakota pork producers in 2019 demonstrated that water flow rate for nipple drinkers was highly variable among barns. Sixty-eight percent had water flow rates above the recommended rate of 500–1,000 mL/min (NSNG, 2010). The objective of this study was to determine the impact of water flow rate on finishing pig performance during the summer months. A total of 396 mixed-sex pigs, in two groups, were utilized in a 77-day trial (34.55 to103.8 kg BW) with 6 pigs/pen. Pens were assigned to one of three water flow rates (high, medium, low) based on the 3-hole diameters of the commercial water nipples used in the facility (2.0, 1.0, 0.80 mm; n = 22 pens/treatment). Daily water usage was recorded for each treatment along with room temperature, outside temperature, and relative humidity. Individual pen water flow rate was recorded every two weeks. At every diet phase change (26± 2.6 days), feed disappearance and individual pig body weight were recorded. Water flow rates averaged 1846±188, 906±214, 508±100 mL/min for high, medium, and low flow rates, respectively. Daily water disappearance for high, medium, and low treatments were 6.8, 2.3, 1.7±3.2 liters/pig, respectively. Final body weight (BW; 103.8±7.4 kg) did not differ. Daily gain (ADG) from 34.5±4.5 to 55.5±4.6 kg BW was greatest (P &lt; 0.05) for high treatment. Daily intake (ADFI) and gain:feed (G:F) from 55.5±4.6 to 79.1±5.3 kg BW were greatest (P &lt; 0.05) for high treatment. Cumulative ADFI was 2.27, 2.18, 2.16±0.16 kg (P &lt; 0.05) in high, medium, and low flow ranges, respectively. There was no differences in cumulative ADG or G:F. Water flow rate had a significant impact on ADFI although there was minimal impact on gain and G:F. Water nipples should be regularly checked as part of normal barn maintenance to ensure adequate, but not excessive, water is available.
133 Influence of Particle Size of Enogen Feed Corn and Conventional Yellow Dent Corn on Nursery and Finishing Pig Performance, Carcass Characteristics and Stomach Morphology
Authors: Williams Hadley , Tokach Mike D , Woodworth Jason C , Goodband Robert D , DeRouchey Joel M , Dritz Steve S , Wecker Haley , Calderon Hilda I 
Published: May 2021

Abstract
Two studies evaluated the effect of particle size of Enogen® Feed corn (Syngenta Seeds, LLC, Downers Grove, IL) and conventional yellow dent corn on nursery and finishing pig performance, carcass characteristics and stomach morphology. In Exp. 1, 360 nursery pigs (DNA 200×400, Columbus, NE; initially 6.6±0.1 kg BW) were used with 5 pigs per pen and 12 pens per treatment. Treatments were arranged in a 2×3 factorial with main effects of corn source (Enogen Feed corn or conventional yellow dent corn) and ground corn particle size (300, 600, or 900 µm). Overall, there was a corn source×particle size interaction (linear, P = 0.027) for G:F ratio. There was no difference due to particle size when pigs were fed conventional yellow dent corn, but in pigs fed Enogen Feed corn, G:F increased with decreasing particle size. Neither corn source nor particle size affected (P &gt; 0.05) ADG or ADFI. In Exp. 2, 323 finishing pigs (241′600; DNA, Columbus, NE; initially 50.0±1.3 kg) were used with 8 or 9 pigs per pen and 6 pens per treatment. Treatments were arranged identical to Exp. 1. Overall, corn source did not elicit differences in ADG, ADFI or G:F (P &gt; 0.05). For corn particle size, ADG and G:F increased (linear, P ≤ 0.014) and ADFI decreased (P = 0.043) as particle size decreased. For carcass characteristics, there was a tendency (linear, P = 0.093) for increased HCW and increased (linear, P = 0.023) carcass yield as corn particle size decreased. For stomach morphology, there was a tendency for a corn source×particle size interaction (P = 0.055) for keratinization score with keratinization increasing linearly (P = 0.001) as particle size decreased for yellow dent corn with no change in keratinization score as particle size decreased for Enogen Feed corn. In summary, reducing corn particle size improved G:F with no major differences observed between corn sources for overall pig performance.
132 Lack of Randomization and Its Impact on Statistical Power and Validity of Statistical Analyses
Authors: Serão Nick V , Neto José Braccini , Tempelman Robert J 
Published: May 2021

Abstract
Allocation of treatments to experimental units (EUs) is done at random. In the presence of a concomitant variable (e.g., initial body weight; iBW), one strategy is to block WUs into iBW groups. However, in some scientific manuscripts, EUs are sorted by iBW and then allocated to treatments (e.g., treatments “A” and “B”) based on iBW, such that the lightest EU receives “A,” the second and third lightest receive “B,” etc. Although this strategy guarantees similar iBW between treatments, this ignores the random process required for statistical analysis of the data. We aimed to quantify the impact of lack of randomization on the statistical power and type I error of completely randomized designs (CRD). Data were simulated for ADG using two treatments (“A” having 50 g/d more than “B,” and MSE=1250 g2/d2). Data were simulated for different replicates per treatment (RepsPerTreat; from 3 to 18, every 3). We used two scenarios for the correlations between iBW and ADG (ρ ADG,iBW): 0 and 0.5. Treatments were allocated to EUs at random (CRD) or according to the order of EUs based on iBW (completely non-randomized design; CNRD). The model included the fixed-effects of intercept and treatment. For ρ ADG,iBW=0, results showed that CRD had greater statistical power (POW) than CNRD for RepsPerTreat from 3 to 9, whereas CNRD had greater from 12 to 18. For ρ ADG,iBW=0.5, CNRD had an even greater POW than CRD starting at 9 RepsPerTreat. Although the type I error (ERROR) of CRD were close to 5% across all scenarios with different RepsPerTreat, CNRD had consistently greater and lower ERROR than CRD with =0 and 0.5, respectively. Having ERROR deviating from 5% is not expected. Visual inspection of the F-values of these models when the null hypothesis was true showed that a distribution other than the theoretical F-distribution, indicating that the statistical test is not valid. Sorting EUs by iBW does not guarantee greater statistical power but results in invalid F-tests.
192 Application of Hemicell HT™ – a β-mannanase Enzyme – Retains Post-weaned Piglet Performance in the Presence of Challenging Protein Sources
Authors: Vangroenweghe Frédéric A , Poulsen Karl 
Published: May 2021

Abstract
β-Mannans are strongly anti-nutritive polysaccharide fibres found in most vegetable feed ingredients. The objective of the study was to compare piglet performance and antibiotic use between a Control group, fed a conventional 3-phase diet, and an Enzyme treated group, fed an adapted 3-phase diet including a β-mannanase enzyme (Hemicell™ HT; Elanco). A seven weeks feeding trial was conducted with 896 pigs in two rotations of 448 piglets in 32 replicate pens of 14 pigs. Two different 3-phase diets were compared: a standard 3-phase control diet and an adapted 3-phase diet including a β-mannanase enzyme included at 300 g/tonne. The following adaptations were made: Phase-1 (weeks 1–2): 1.14% potato protein concentrate and 1.00% Forcital (extruded soya product) were replaced with soybean meal. Phase-2 (weeks 3–4): 0.46% potato protein concentrate and 0.68% Forcital were replaced with soybean meal. Phase-3 (weeks 5–7): β-mannanase was formulated to replace 63 kcal/kg NE. Standard piglet performance parameters (ADWG, ADFI, FCR) and antibiotic use were recorded. All data analyses were performed using R version 3.6.3 (R Core Team, 2020). All tests were performed at the 5% level of significance. When multiple testing was involved, the nominal 5% Familywise Error Rate (FWER) was used. Throughout the trial and within each phase, ADWG, ADFI and FCR were not significantly different (P &gt; 0.05) between Control and Enzyme group. Mortality was significantly (P &lt; 0.001) lower (-0.90 %) in the Enzyme treated group. Antimicrobial use was significantly (P &lt; 0.01) lower (-56%) in the Enzyme treated group as compared to the Control group. Inclusion of a β-mannanase to nursery diets with an adapted formulation by replacing expensive protein sources by soybean meal, or reducing the NE content by 63 kcal/kg, resulted in similar piglet performance post-weaning with reduced mortality and less antimicrobials used.
193 Substitution of Expensive Protein Sources by Soybean Meal Supplemented with a β-mannanase Enzyme Results in Improved General Clinical Health Score During the Post-weaning Period
Authors: Vangroenweghe Frédéric A , Poulsen Karl 
Published: May 2021

Abstract
β-Mannans are strongly anti-nutritive polysaccharide fibres found in most vegetable feed ingredients. The objective of the study was to compare piglet performance and antibiotic use between a Control group, fed a conventional 2-phase diet, and an Enzyme treated group, fed an adapted 2-phase diet including a β-mannanase enzyme (Hemicell™ HT; Elanco). A seven-week feeding trial was conducted with 320 pigs in two rotations of 160 piglets in 20 replicate pens of 8 pigs. Two different 3-phase diets were compared: a standard 3-phase control diet and an adapted 3-phase diet including a β-mannanase enzyme included at 300 g/tonne. The following adaptation were made: Phase-1 (weeks 1–3): 0.15% potato protein concentrate and 2.00% Danex [extruded soybean meal (SBM)], was replaced with SBM 48%, Phase-2 (weeks 4–7): β-mannanase was formulated to replace 63 kcal/kg NE. Standard piglet performance parameters (ADWG, ADFI, FCR) and antibiotic use were recorded. All data analyses were performed using R version 3.6.3 (R Core Team, 2020). All tests were performed at the 5% level of significance. When multiple testing was involved, the nominal 5% Familywise Error Rate (FWER) was used. Throughout the trial and within each phase, ADWG, ADFI and FCR were not significantly different (P &gt; 0.05) between Control and Enzyme group. No mortality occurred and no antimicrobials were used in either of the treatment groups. Inclusion of a β-mannanase in nursery diets with an adapted formulation, by replacing expensive protein sources by soybean meal, or reducing the NE content by 63 kcal/kg, resulted in similar piglet performance post-weaning with reduced mortality and less antimicrobials used.
195 Does Feeding a Protease and Probiotic (Bacillus Subtilis) Combination (Syncra® SWI) Result in Improved Growth Performance and Mortality in a Commercial Wean-to-finish Swine Feeding Program?
Authors: Kellner Trey A , Ellingson Josh , de Souza Ana L P , Remus Janet C 
Published: May 2021

Abstract
The response to probiotics and enzymes is often documented in research facilities with a high degree of control and via pigs with no insults to health or feed intake. However, in commercial conditions, the response to feed additives promoting advanced gut health and improved digestion must be consistent and defined over a wide range of health statuses, stocking densities, feed intakes, environments, and diet formulations. The objective of this experiment was to determine if a combined feed protease and probiotic system (Syncra® SWI 201, DuPont, Wilmington, DE) would improve growth performance and mortality under commercial conditions. A total of 127,092 pigs (6.0 ± 0.1 kg; PIC 337 sired, Hendersonville, TN) from a sow farm producing porcine reproductive and respiratory virus and rotavirus positive weaned pigs were placed in 53 2,400-head commercial wean-to-finish barns that were alternated to 1 of 2 treatments (a control treatment without Syncra® SWI (SSWI) or with SSWI included at 72.6 g/ton of finished feed from 22.7 kg of BW to harvest) in a rolling allotment over a 12-month period. Pigs were on the experiment for an average of 162 ± 1.0 days (until harvest). Throughout the 12-month experimental period, diets (outside of the SSWI inclusion) could change in order to maximize return over feed costs. Data were analyzed using Proc MIXED (SAS 9.4; Cary, NC) with treatment as the main effect and barn as the experimental unit. Compared to the control, adding SSWI improved mortality by 1.9% and percent grade 1 marketed pigs by 2.2% (P ≤ 0.025). Compared to the control, SSWI did not improve ADG (control = 0.75 vs. SSWI = 0.78 kg) or gain:feed (control = 0.401 vs. SSWI = 0.396). In conclusion, the inclusion of the SSWI combined feed protease and probiotic system can improve mortality and grade 1 marketed pigs, but not growth performance under commercial conditions.
197 Supplementation of Bacillus Amyloquefaciens on Growth Performance and Diarrhea Score of Weaned Pigs Experimentally Infected with a Pathogenic E. coli
Authors: Jinno Cynthia , Wong Braden T , Kluenemann Martina , Li Xunde , Liu Yanhong 
Published: May 2021

Abstract
The objective of this experiment was to investigate the effects of dietary supplementation of Bacillus amyloquefaciens on diarrhea and growth performance of weaned pigs experimentally infected with a pathogenic E. coli. A total of 50 weaned pigs (7.41 ± 1.35 kg) were individually housed in disease containment rooms and randomly assigned to one of the 5 treatments: sham control (CON-), sham B. amyloquefaciens (BAM-), challenged control (CON+), challenged B. amyloquefaciens (BAM+) and challenged carbadox (CAR+). The experiment lasted 28 days with 7 days’ adaptation and 21 days after the first E. coli inoculation. The doses of F18 E. coli inoculum were 1010 CFU/3 mL oral dose daily for 3 consecutive days. Pigs were weighed on d -7, and d 0, 7, 14, and 21 PI and feed intake was recorded weekly to calculate growth performance. Diarrhea score was recorded twice daily ranging score from 1 (normal feces) to 5 (watery diarrhea). Fecal swabs were performed on each pig on d 2, 7, 14, and 21 PI to test the percentage of β-hemolytic coliforms in total coliforms. All data were analyzed with PROC MIXED of SAS. E. coli challenge reduced (P &lt; 0.05) growth rate through the entire experiment. However, supplementation of CAR enhanced (P &lt; 0.05) growth performance, while inclusion of BAM tended (P &lt; 0.10) to increase growth performance of weaned pigs. No differences were observed in growth performance of pigs between BAM+ and CAR+ throughout the experiment. E. coli challenge did not change the percentage of β-hemolytic coliforms but increased (P &lt; 0.05) frequency of diarrhea. However, no difference was observed in diarrhea among 3 dietary treatments. In conclusion, supplementing B. amyloquefaciens tended to enhance growth performance but had limited effects on diarrhea of weaned pigs challenged with E. coli.