bluuebunny/crossref_metadata_2025_split huggingface dataset:
Protein-Metabolite Interactomics Reveals Novel Regulation of Carbohydrate Metabolism
Authors: Hicks Kevin G. , Cluntun Ahmad A. , Schubert Heidi L. , Hackett Sean R. , Berg Jordan A. , Leonard Paul G. , Ajalla Aleixo Mariana A. , Blevins Aubrie , Barta Paige , Tilley Samantha , Fogarty Sarah , Winter Jacob M. , Ahn Hee-Chul , Allen Karen N. , Block Samuel , Cardoso Iara A. , Ding Jianping , Dreveny Ingrid , Gasper Clarke , Ho Quinn , Matsuura Atsushi , Palladino Michael J. , Prajapati Sabin , Sun PengKai , Tittmann Kai , Tolan Dean R. , Unterlass Judith , VanDemark Andrew P. , Vander Heiden Matthew G. , Webb Bradley A. , Yun Cai-Hong , Zhap PengKai , Hill Christopher P. , Nonato Maria Cristina , Muller Florian L. , Gottschling Daniel E. , Cox James E. , Rutter Jared 
Published: August 2021

AbstractMetabolism is highly interconnected and also has profound effects on other cellular processes. However, the interactions between metabolites and proteins that mediate this connectivity are frequently low affinity and difficult to discover, hampering our understanding of this important area of cellular biochemistry. Therefore, we developed the MIDAS platform, which can identify protein-metabolite interactions with great sensitivity. We analyzed 33 enzymes from central carbon metabolism and identified 830 protein-metabolite interactions that were mostly novel, but also included known regulators, substrates, products and their analogs. We validated previously unknown interactions, including two atomic-resolution structures of novel protein-metabolite complexes. We also found that both ATP and long-chain fatty acyl-CoAs inhibit lactate dehydrogenase A (LDHA), but not LDHB, at physiological concentrations in vitro. Treating cells with long-chain fatty acids caused a loss of pyruvate/lactate interconversion, but only in cells reliant on LDHA. We propose that these regulatory mechanisms are part of the metabolic connectivity that enables survival in an ever-changing nutrient environment, and that MIDAS enables a broader and deeper understanding of that network.
Signaling network model of cardiomyocyte morphological changes in familial cardiomyopathy
Authors: Khalilimeybodi Ali , Riaz Muhammad , Campbell Stuart G. , Omens Jeffrey H. , McCulloch Andrew D. , Qyang Yibing , Saucerman Jeffrey J. 
Published: August 2021

AbstractFamilial cardiomyopathy is a precursor of heart failure and sudden cardiac death. Over the past several decades, researchers have discovered numerous gene mutations primarily in sarcomeric and cytoskeletal proteins causing two different disease phenotypes: hypertrophic (HCM) and dilated (DCM) cardiomyopathies. However, molecular mechanisms linking genotype to phenotype remain unclear. Here, we employ a systems approach by integrating experimental findings from preclinical studies (e.g., murine data) into a cohesive signaling network to scrutinize genotype to phenotype mechanisms. We developed an HCM/DCM signaling network model utilizing a logic-based differential equations approach and evaluated model performance in predicting experimental data from four contexts (HCM, DCM, pressure overload, and volume overload). The model has an overall prediction accuracy of 83.8%, with higher accuracy in the HCM context (90%) than DCM (75%). Global sensitivity analysis identifies key signaling reactions, with calcium-mediated myofilament force development and calcium-calmodulin kinase signaling ranking the highest. A structural revision analysis indicates potential missing interactions that primarily control calcium regulatory proteins, increasing model prediction accuracy. Combination pharmacotherapy analysis suggests that downregulation of signaling components such as calcium, titin and its associated proteins, growth factor receptors, ERK1/2, and PI3K-AKT could inhibit myocyte growth in HCM. In experiments with patient-specific iPSC-derived cardiomyocytes (MLP-W4R;MYH7-R723C iPSC-CMs), combined inhibition of ERK1/2 and PI3K-AKT rescued the HCM phenotype, as predicted by the model. In DCM, PI3K-AKT-NFAT downregulation combined with upregulation of Ras/ERK1/2 or titin or Gq protein could ameliorate cardiomyocyte morphology. The model results suggest that HCM mutations that increase active force through elevated calcium sensitivity could increase ERK activity and decrease eccentricity through parallel growth factors, Gq-mediated, and titin pathways. Moreover, the model simulated the influence of existing medications on cardiac growth in HCM and DCM contexts. This HCM/DCM signaling model demonstrates utility in investigating genotype to phenotype mechanisms in familial cardiomyopathy.
Local mechanical stimuli shape tissue growth in vertebrate joint morphogenesis
Authors: Comellas Ester , Farkas Johanna E , Kleinberg Giona , Lloyd Katlyn , Mueller Thomas , Duerr Timothy J , Muñoz Jose J , Monaghan James R , Shefelbine Sandra J 
Published: August 2021

AbstractThe correct formation of synovial joints is essential for proper motion throughout life. Movement-induced forces are critical to creating correctly shaped joints, but it is unclear how cells sense and respond to these mechanical cues. To determine how mechanical stimuli drive joint morphogenesis, we combined experiments on regenerating axolotl (Ambystoma mexicanum) forelimbs with a poroelastic model of bone rudiment growth. Animals either regrew forelimbs normally (control) or were injected with a TRPV4 agonist to impair chondrocyte mechanosensitivity during joint morphogenesis. We quantified growth and shape in regrown humeri from whole mount light sheet fluorescence images of the regenerated limbs. Results revealed statistically significant differences in morphology and cell proliferation between groups, indicating that mechanical stimuli play a role in the shaping of joints. We simulated local tissue growth in a finite element model with a biological contribution to growth proportional to chondrocyte density, and a mechanical contribution to growth proportional to fluid pressure. Computational predictions agreed with experimental outcomes, suggesting that interstitial pressure driven from cyclic mechanical stimuli promotes local tissue growth. Predictive computational models informed by experimental findings allow us to explore potential physical mechanisms involved in tissue growth to advance our understanding of the mechanobiology of joint morphogenesis.
Tip growth in the brown alga Ectocarpus is controlled by a RHO-GAP-BAR domain protein independently from F-actin organisation
Authors: Nehr Zofia , Chenivesse Sabine , Billoud Bernard , Genicot Sabine , Desban Nathalie , Theodorou Ioannis , Nasir Adeel , Le Bail Aude , Rabillé Hervé , Godfroy Olivier , Katsaros Christos , Charrier Bénédicte 
Published: August 2021

AbstractThe brown alga Ectocarpus is a filamentous seaweed that grows by tip growth and branching. In the morphometric mutant etoile, tip growth is slower than in the WT and eventually stops. In this paper, we show that the causal etoile mutation is a null mutation in a bi-domain BAR-RhoGAP gene. By quantitative RT-PCR, we showed that ETOILE is ubiquitously expressed in prostrate filaments of the Ectocarpus sporophyte, and is downregulated in the etoile mutant. We immunolocalised both domains of the protein in WT and etoile, as well as RAC1, the known target of Rho-GAP enzymes. Thus, ETOILE would be localised at the apical cell dome where it would control the localisation of EsRAC1 to the plasma membrane. Actin staining showed that the mutant is not affected in F-actin structures. Overall, these results suggest that in Ectocarpus, BAR-RhoGAP controls tip growth by controlling RAC1 localization and through an actin-independent mechanism.
Effect of urea–formaldehyde nanoparticles on the barrier property and delamination resistance for epoxy coating
Authors: Li Zhe , Zheng Hongpeng , Liu Li , Meng Fandi , Cui Yu , Wang Fuhui 
Published: August 2021

Purpose
The purpose of this paper is to investigate the effect of urea–formaldehyde (UF) nanoparticles on the barrier property and delamination resistance for epoxy coating in a 3.5% NaCl aqueous solution.

Design/methodology/approach
The UF resin was synthesized via sol–gel method, and UF/epoxy composite coating was prepared through ball-milling process; the microstructure and chemical composition of UF resin were observed using the Fourier transform infrared spectroscopy, scanning electron microscopy and transmission electron microscopy; the bonding strength of coating/metal interface was investigated through adhesion test; the mechanical properties of the coatings were studied by tensile tests; and the barrier and corrosion resistance properties were verified using salt spray test, cathodic delamination test and electrochemical impedance spectroscopy measurements.

Findings
The experimental results indicated that the UF resin presented uniformly dispersed nanoparticles in the epoxy matrix and enhanced the bonding strength of coating/metal interface and then improved the delamination resistance for composite coating, which resulted in the enhancement of the barrier property and corrosion resistance for UF/epoxy composite coating.

Originality/value
In this paper, an easily prepared blending compounding coating with excellent corrosion resistance property was synthesized via sol–gel method and ball-milling process. The effects of UF nanoparticles on the barrier property and delamination resistance were investigated in detail.
Evaluation of enterprise technology innovation capability in prefabricated construction in China
Authors: Dou Yudan , Xue Xiaolong , Wang Yuna , Xue Weirui , Huangfu Wenbo 
Published: August 2021

Purpose
This study aims to evaluate enterprise technology innovation capability in prefabricated construction (PC) from an input-output perspective, using six integrated enterprises in China as cases.

Design/methodology/approach
An evaluation system for enterprise technology innovation capability in PC was constructed, including total input, technology output (TO) and project output. All the evaluation indexes were quantified, and the subject and object indexes weights were determined using the fuzzy cognitive map and information entropy, respectively. The final scores and ranks were evaluated through gray relational analysis (GRA) based on the combined weights.

Findings
It was found that enterprise technology innovation capability in PC was low in China, with its unbalanced development in different dimensions and the poorest performance in TO, currently.

Originality/value
This research has developed an evaluation system for technology innovation capability in PC at the enterprise level and scientifically quantified all the indexes, which is a breakthrough over existing studies. The GRA model based on the combined weights proposed in this study can be applied to other comparable fields and regions, with its easy operation.
Operational challenges during a pandemic: an investigation in the electronics industry
Authors: Paul Sanjoy Kumar , Chowdhury Priyabrata , Chowdhury Md. Tarek , Chakrabortty Ripon Kumar , Moktadir Md. Abdul 
Published: August 2021

PurposeThe recent coronavirus disease 2019 (COVID-19) pandemic poses numerous challenges to supply chains. This pandemic is quite unique when compared to previous epidemic disruptions and has had a severe impact on supply chains. As a result, the operational challenges (OCs) caused by COVID-19 are still unknown among practitioners and academics. It is critical to comprehensively document current OCs so that firms can plan and implement strategies to overcome them. Consequently, this study systematically identifies and ranks COVID-19-related OCs.Design/methodology/approachThis study uses an integrated methodology combining expert interviews and the best-worst method (BWM) to analyze the results. The data have been collected from the electronics industry of Bangladesh, an emerging economy. This study also conducts a sensitivity analysis to check the robustness of the results.FindingsThe results reveal 23 COVID-19-related OCs under five categories: sourcing, production and inventory management, demand management and distribution, return management and after-sales service, and supply chain-wide challenges. The quantitative investigation reveals that overstock in finished goods inventory, low end-customer demands, order cancellations from dealers and retailers, high inventory holding costs and lack of transportation are the top five OCs.Practical implicationsThe findings will help practitioners to understand the OCs and allow them to prepare for future major disruptions and formulate long-term strategies for operations during and after the COVID-19 pandemic.Originality/valueThis study contributes to the literature on supply chain complexity and challenges by considering a major pandemic outbreak. Moreover, the study also contributes to the knowledge on emerging economies, which have been largely neglected in the current literature.
Understanding message (de)coding in multi-lingual slogans: industrial perspectives from Turkey and Russia
Authors: Çal Betül , Hüseyinli Tahire 
Published: August 2021

PurposeThe main goal of the study is to investigate how same-brand slogans simultaneously in use in two emerging markets, namely Turkey and Russia, differ semantically. The study further examines in what ways the industrial competition structure impacts the semantic slogan design within these two contexts.Design/methodology/approachThe study uses the method of semantic explication that is based on a 19-device taxonomy. This method is applied to 56 slogan pairs in the Turkish and Russian languages launched for the same brands/products across 6 industries.FindingsResults indicate that same-brand slogans differ semantically between Turkey and Russia. Moreover, firms tend to conform to a shared semantic pattern within a given industry, largely depending on the industrial competition structure. While strong local competition (as in the electronics and cleaning products industries in Turkey and in the personal care and beverages industries in Russia) leads firms to use self-reference, international competition (as in the automotive, personal care and beverages industries in Turkey and in the electronics and cleaning products industries in Russia) promotes them to use hyperbole in their slogan design.Practical implicationsAdopting a common semantic pattern within an industry may carry the risk of restricting brand differentiation and consumers' sense of novelty. Furthermore, the inclusion of brand names in slogans may make slogans sound assertive and lead consumers to overreact to the brand.Originality/valueUnlike many studies exploring different-brand slogans through a syntactic or grammatical lens, this study investigates the semantic features of same-brand slogans launched in two emerging market contexts. It adopts a B2B perspective, unlike many extant studies that often focus on a B2C one.
Effect of advertising expenditures on analyst coverage: evidence from an emerging market
Authors: Farooq Omar , Satt Harit , Bendriouch Fatimazahra 
Published: August 2021

PurposeThis paper aims to document the relationship between advertising expenditures and analyst coverage in a sample of Indian firms during the period between 2000 and 2019.Design/methodology/approachIn order to test the effect of advertising expenditures on the extent of analyst coverage, the authors estimate various versions of pooled ordinary least squares (OLS) regression. The dependent variable (ANALYST) measures the total number of analysts covering a firm in a given year. The main independent variable of interest in this paper represents the advertising activity. The authors define the extent of advertising activity (ADVERT) as the ratio of total advertising expenditures and total assets.FindingsThe study’s results show that advertising expenditures have a significantly positive impact on the extent of analyst coverage and are robust across various proxies of the key variables and various estimation procedures.Practical implicationsThere are a number of key takeaways from our study. First, firms that expend more resources on advertising are more likely to be followed by analysts which is associated with better performance, lower information asymmetries associated and high advertising expenditures. Second, stock prices with more information embedded in them may signify that these firms receive more attention from investors and have lower information asymmetries. And finally the impact of advertising on the decision of an analyst to cover a firm becomes more pronounced for firms with high stock price synchronicity. All these three main conclusions are giving investors a clear insight on analyst coverage, advertising expenditure and the link between the two.Originality/valueThe results are consistent with the argument that advertising expenditures induces analysts to cover firms because firms with high advertising activities are more likely to have better performance, lower information asymmetries and increased attention from investors. All of these factors are supposed to facilitate the analyst coverage.
Lateral loading performance of end narrow panel reinforced light wood frame shear walls
Authors: Di Jing , Zuo Hongliang 
Published: August 2021

PurposeThe sheathing panels of traditional light wood frame shear walls mainly use oriented strand board (OSB) panels, and the damage of the traditional walls is mainly caused by the tear failure at the bottom corner of the OSB panel. In order to improve the lateral performance of the traditional light wood frame shear wall, a new type of end narrow panels reinforced light wood frame shear wall is proposed.Design/methodology/approachThe monotonic loading tests and finite element analysis of nine groups of walls, with different types of end narrow panel, types of fasteners used on the end narrow panels and the end narrow panels edge fastener spacing, are carried out. The effects of different characters on lateral performance of light wood frame shear walls are reported and discussed.FindingsThe failure modes of the wall reinforced by parallel strand bamboo narrow panels with 150 mm edge nails spacing are similar to the traditional wall. Conversely, the failure modes of other groups of walls reinforced by end narrow panels are the tears of the bottom narrow panel or the bottom beam. The end narrow panel reinforced light wood frame shear wall can make full use of the material property of sheathing panels. Compared with the lateral performance of traditional walls, the new-type end narrow panels reinforced walls have better lateral performance.Originality/valueA new type of end narrow panels reinforced light wood frame shear wall is proposed, which can enhance the lateral performance of the traditional light wood frame shear wall. The new-type walls have advantages of convenient operation, manufacture cost saving and important value of engineering application.
Remote working and employee engagement: a qualitative study of British workers during the pandemic
Authors: Adisa Toyin Ajibade , Ogbonnaya Chidiebere , Adekoya Olatunji David 
Published: August 2021

PurposeThrough the lens of Conservation of Resources (COR) theory, this study explores how remote working inhibits employee engagement. The authors offer a fresh perspective on the most salient work- and nonwork-related risk factors that make remote working particularly challenging in the context of Covid-19.Design/methodology/approachThe authors use data from semi-structured interviews with 32 employees working from home during the Covid-19 lockdown. Based on the interpretivist philosophical approach, the authors offer new insights into how employees can optimize work- and nonwork-related experiences when working remotely.FindingsThe authors show that the sudden transition from in-person to online modes of working during the pandemic brought about work intensification, online presenteeism, employment insecurity and poor adaptation to new ways of working from home. These stress factors are capable of depleting vital social and personal resources, thereby impacting negatively on employee engagement levels.Practical implicationsEmployers, leaders and human resource teams should be more thoughtful about the risks and challenges employees face when working from home. They must ensure employees are properly equipped with the relevant resources and support to perform their jobs more effectively.Originality/valueWhile previous research has focused on the benefits of remote working, the current study explores how it might be detrimental for employee engagement during a pandemic. The study provides new evidence on the most salient risks and challenges faced by remote workers, and how the unique Covid-19 context has made them more pronounced.
Turning disruption into growth opportunity: the red team strategy
Authors: Sun Sunny Li , Zhang Yanli , Zhu Zhu 
Published: August 2021

Purpose
The purpose of this paper is to propose a broadened and integrated red team strategy with concrete steps to help companies better deal with the disruptive forces prevalent in the world today and turn disruptions into growth.

Design/methodology/approach
This paper synthesizes and builds on previous research and relevant business cases accumulated through our research and executive teaching experience. The authors offer a broadened and integrated red team strategy with practical guidance for business executives

Findings
The authors provide four key steps to help companies implement the red team strategy: create a red team culture and encourage diverse perspectives; establish an independent red team to overcome organizational inertia; use the red team to embrace disruption and growth opportunities; and take a milestone approach to red team execution and resource allocation.

Research limitations/implications
More research on red team strategy is needed to delve into the underlying factors and delineate the boundary conditions for specific details in this strategy and implementation.

Practical implications
The red team strategy provides concrete steps to help companies in their efforts to adapt to and capitalize on disruptive forces.

Originality/value
The red team strategy extends the concept and application of red teams and integrates previously fragmented ideas and practices into a systematic model with simple steps, which make it easier for companies to cope with disruption.
Online self-radicalisation: a case study of cognitive vulnerabilities for radicalization to extremism and single actor terrorism
Authors: Little Ryan , Ford Peter , Girardi Alessandra 
Published: August 2021

Purpose
Understanding the psychological risk factors in radicalisation and terrorism is typically limited by both a lack of access to individuals who carry out the acts and those who are willing to engage in research on the matter. The purpose of this study is to describe the process of self-radicalisation of an otherwise law-abiding individual who engaged in single-actor terrorism activities.

Design/methodology/approach
A single case study, based on clinical interviews and psychometric testing, of an individual with autism who engaged in multiple acts of terrorism through online activity. The case is presented within existing frameworks of radicalisation, and describes how it developed along the steps described in the path to intended violence.

Findings
A number of variables are identified as contributing towards the individual’s vulnerability to radicalisation, such as deficits in higher order cognition, psychopathology, autism spectrum disorder traits, personal interests, social isolation and life stressors.

Originality/value
Unique to this study is how the process of radicalisation and the possibility to carry out the individual’s attacks was made possible only through the use of internet technology.