bluuebunny/crossref_metadata_2025_split huggingface dataset:
A rapid and efficient method to evaluate the infection of major DNA viruses in sweet potato seedlings and tubers
Authors: LIU Qili , Zhou Feng , Song Puwen , Li Yongqiang 
Published: August 2021

Abstract
Background

Sweet potato is an important food crop in China which is the fifth largest staple crop next to rice, wheat, maize, and soybean. Recent years the destroy infecting by DNA viruses in sweet potato was more and more serious. Three DNA viruses (sweepoviruses, Badnavirus and Mastrevirus) are major agents in viral diseases of sweet potato in China. It is necessary to establish a rapid and efficient method to evaluate the health of sweet potato seedlings and tubers,which will greatly affect the yield, quality of tubers and seeding circulation of sweet potatoes. Sweepoviruses were a group phylogenetically distinct from other begomoviruses that infects plants of the family Convolvulaceae including sweet potato. Sweet potato symptomless virus 1 (SPSMV-1) is the only species of Mastrevirus which can infected sweet potato. Sweet potato Badnavirus B (SPBV-B) are non-enveloped bacilliform DNA viruses with a monopartite genome which belonged to the genus Badnavirus of the family Caulimoviridae and was first found in sweet potato in 2009. As the mixed infection of these viruses is very common, rapid detection is required for etiologic diagnosis.
Results

In this study, a rapid and efficient method to evaluate the infection of major DNA viruses (sweepoviruses, Badnavirus and Mastrevirus) in sweet potato seedlings and tubers was developed and applied. A mixture of three pairs of primers was used for amplification of viral nucleic acids, yielding three different amplicons with sizes of 750 bp, 147 bp and 396 bp for sweepoviruses, Badnavirus and Mastrevirus respectively. The specificity and sensitivity of multiplex PCR were also evaluated. A total of 65 sweet potato samples with virus-like symptoms cuttings and storage roots from Henan province in China were collected between June 2019 and July 2021. They were tested for the presence of three DNA viruses by multiplex PCR which showed sweepoviruses, Badnavirus and Mastrevirus infections were 60.0%, 36.7%, and 43.3% respectively. Co-infections with three viruses, sweepoviruses and Badnavirus, sweepoviruses and Mastrevirus, Badnavirus and Mastrevirus were identified in different samples (the detection ratio of co-infections was 13.3%, 20.0%, 13.3%, and 10.0% respectively).
Conclusions

In the current situation of frequent virus mixed infection in sweet potatoes, this method is the first report on a simple assay and may be a potentially useful for apid and efficient method to evaluate the infection of major DNA viruses in sweet potato seedlings and tubers in China.
Aspirin Regulates Gemcitabine Resistance in Pancreatic Cancer via Inhibiting the PI3K/Akt/mTOR Signaling Pathway and Reversing Epithelial-mesenchymal Transition
Authors: zhou hanyu , Yan ZhiYing , Wei Feifei , Liu Muhan , liu yi , Zhang Xianming , xu Kequn , Zhu Yingwei , yun xiao 
Published: August 2021

Abstract
Background: Gemcitabine is considered a classical agent for the treatment of patients with pancreatic cancer. However, gemcitabine resistance is a common cause of treatment failure, leading to poor survival. Therapy to overcome gemcitabine resistance would benefit patients with pancreatic cancer. This study investigated the impact of aspirin (ASA) to gemcitabine resistance in the biological function of pancreatic cancer cells and the potential mechanism. Methods: The MTT assay, wound healing assay and annexin V-FITC/PI test was used to determine whether ASA could inhibit gemcitabine resistance in pancreatic cancer cells. Besides, the expression of Bcl-2, Bax, E-cadherin, Vimentin, p-PI3K, p-AKT and p-mTOR was detected by the Western blot. Statistically significant differences between groups were determined with the Student’s t-test. Results: The proliferation and migration of SW1990 and BxPC3 cells were significantly decreased while the apoptosis rate was increased in the combination of ASA and gemcitabine group (p<0.05). The level of Bcl-2 was decreased and the level of Bax was increased significantly in the combination of ASA and gemcitabine group (p<0.05) while the level of p-PI3K, p-AKT and p-mTOR was decreased (p<0.05). What’s more, this group significantly reversed EMT in SW1990 and BxPC3 cells with an increase in the expression of E-cadherin and a decrease in Vimentin. Conclusion: Our research provided evidence ASA could help to inhibit gemcitabine resistance of pancreatic cancer cells, probably via inhibiting the PI3K/AKT/mTOR pathway and reversing EMT. Thus, combined use of ASA and gemcitabine is expected to be a potential therapeutic strategy for pancreatic cancer patients.
Discussion On The Mechanism of Que In The Treatment of Breast Neoplasms And Immune Prevention And Treatment
Authors: Qiu Dan , Yan Xianxin , Xiao Xinqin , Zhang Guijuan , Wang Yanqiu , Cao Jingyu , Ma Ruirui , Hong Shouyi , Ma Min 
Published: August 2021

Abstract
Background: The precancerous disease of breast cancer is an inevitable stage in the emergence and development of breast neoplasms. Breast cancer (BC) is a common malignant tumor in female worldwide. A large number of literatures have proved that, as antitumor drugs, flavonoid compounds can promote proliferation and immune regulation of T cell. Many researchers believe that Quercetin (Que) has great potential in the field of anti-breast cancer. Besides that, γδ T cells are a class of non-traditional T cells, which have long attracted attention due to their potential in immunotherapy. Above all, JAK/STAT1 signaling pathway is closely related to the immunity.MethodsIn the experiment designed in this paper, we first used Que, one of the flavonoids, to screen the target gene. Then, MCF-10A, MCF-10AT, MCF-7 and MDA-MB231 BC cells were co-cultured with Que for 24h and 48h, apoptosis was found in some the cells. We then cultured Que with γδ T cells and found that Que can promote the proliferation of Vδ2 T cell subsets of γδ T cells, thus enhancing the killing effect of γδ T cells. Western blot was use to showed the change of JAK/STAT1 signaling pathway related proteins after the Que was co-cultured with MCF-10AT and MCF-7 for 48h.ResultsNetwork pharmacology has shown that Que related pathways include the JAK/STAT1 signaling pathway and are associated with precancerous breast cancers. Que induced apoptosis of MCF-10AT, MCF-7 and MDA-MB-231 in a time and concentration-dependent manner. Most importantly, Que can promote the differentiation of γδ T cells into the Vδ2 T cell subpopulation, this means that Que and γδ T cells may play a synergistic role in killing tumor cells and cellular immune regulation. In addition, our results showed that Que can increase in protein levels of IFNγ-R, p-JAK2 and p-STAT1, while the concomitant decrease protein levels of PD-L1.ConclusionsIn conclusion, Que plays a synergistic role in killing BC cells and promoting apoptosis by regulating the expression of IFNγ-R, p-JAK2, p-STAT1, and PD-L1 in the JAK/STAT1 signaling pathway and promoting the regulation of γδ T cells. Que may be a potential drug for the prevention of precancerous breast cancer and adjuvant treatment of BC.
A study of antimicrobial activities of aqueous and ethanolic extract of bee pollen against
Authors: Jilo Kasim Roba 
Published: August 2021

Abstract
Background

Ethiopia is one of the plant species-rich countries in the world and the center of origin of many medicinal plants. Studying antimicrobial activities of pollen is vivacious to investigate plant resources for medicinal values and the study was conducted to evaluate antimicrobial properties of bee pollen against mentioned bacteria.
Methods

Completely Randomized Design was used for laboratory work. After adjusting turbidity, consistent growth of bacterial culture was made using a sterilized cotton swab. 20 grams of bee pollen was added to 200 ml of distilled water as well as ethanol and finally, the extract was filtered by Whatman filter by paper, dried and weighted and stock solutions were made as follows,3.6 gm. was added to 12 ml of distilled water to prepare stock solutions as follows 3.6:12 = 0.3 x \({10}^{6}\) = 3 x \({10}^{5}\) ppm stock solution and antimicrobial activities of pollen were tested against mentioned bacteria. Data were imported to R software version 3.44. Multilevel analysis was used to see the interaction between bacteria species and each concentration of pollen and Anova was used to see the significance of these concentrations on bacteria species. A p-value of < 0.05 was considered statistically significant.
Results

Results indicated that bacteria were more inhibited at 72 hours than 48 and 24 hours and the results showed an ethanolic extract of bee pollen had antimicrobial activities against both Gram-negative and Gram-positive bacteria mentioned above. Time has significant effects on tested bacteria (p = 0.000) and treatments have significant effects on tested organisms (p = 0.000). The ethanolic extract inhibited the growth of more Gram-negative bacteria: Escherichia coli and Shigella boydii. Bacillus subtilis was mostly inhibited by aqueous extract of bee pollen than others.
Conclusions

Ethanolic extract of pollen had antibacterial activities against all tested bacterial strains even though it is concentration and time-based. The ethanolic extract inhibited more Gram-negative bacteria relatively while aqueous extract inhibited more Gram-positive relatively. Negative controls (sterilized water) didn’t show any antimicrobial properties, while positive control (Chloramphenicol) had antimicrobial activities. Further isolation and characterization of bioactive compounds from pollen are useful to develop a novel botanical formulation for further applications from the pollen of medicinal plants.
Levels, Variations and Sources of 90sr and 137cs in Environmental and Food Samples Around Qinshan Nuclear Power Plant, China in 2012-2019
Authors: Cao Yiyao , Zhao Zhixin , Lou Xiaoming , Yu Shunfei , Zhang Meibian , Xuan Zhiqiang , Lai Zhongjun , Gao Xiangjing , Zhao Yaoxian , Ren Hong , Zhang Dongxia , Wang Peng 
Published: August 2021

Abstract
Environmental radioactivity monitoring in the surroundings of nuclear facilities is important to provide baseline data for effective detection in case of any radioactive release in the region. In this work, we report for the first time the long-term monitoring data of 137Cs and 90Sr in environmental and food samples around Qinshan Nuclear Power Plant (QNPP) in 2012-2019. The distribution levels, temporal variations and source terms of 137Cs and 90Sr in the investigated samples were discussed. The annual effective dose (AED) for the local population from the ingestion of foods was also evaluated. Peak values of 90Sr and 137Cs concentrations and 137Cs/90Sr radioactivity ratio were observed in total atmospheric deposition in 2016 and some water and food samples in the following years. This seems to be associated to an additional radioactive input, mostly likely from the operational release of a local facility. This demonstrates that 90Sr and 137Cs, especially the 137Cs/90Sr activity ratio, are sensitive indicators for detecting potential radioactive releases. Nevertheless, overall 90Sr and 137Cs activity concentrations measured during 2012-2019 in this work were at the background levels with average AED far below the internationally permissible limit and recommendation.
Unique Cerebrospinal Fluid Profiles of Patients With Human Immunodeficiency Virus- Associated Cryptococcal Meningitis With a Ventriculoperitoneal Shunt : A Retrospective Cohort Study
Authors: Tao Ran , Xu Lijun , Guo Yongzheng , Xu Xiaoke , Zheng Jiesheng , Zhu Biao 
Published: August 2021

Abstract
Background: The long-term complications of ventriculoperitoneal shunting in patients with human immunodeficiency virus-associated cryptococcal meningitis remain unclear. We conducted a case-control study investigating the long-term effects of ventriculoperitoneal shunting in patients with human immunodeficiency virus-associated cryptococcal meningitis. Methods: Between January 2011 and December 2019, 112 patients with human immunodeficiency virus-associated cryptococcal meningitis from our hospital were enrolled in this retrospective cohort study. Of those, 30 (26.8%) patients underwent ventriculoperitoneal shunting (VPS group); the remaining (n = 82; 73.2%) were included in the non-VPS group. Survival was estimated using the Kaplan–Meier method. Univariate and multivariate Cox regression analyses were performed to identify factors associated with ventriculoperitoneal shunting. Results: The VPS group (n=21) had lower cerebrospinal fluid glucose (2.51±0.81 vs. 3.16±0.48 mmol/L; P=0.002) and higher cerebrospinal fluid protein levels (1.37 [0.83–1.49] vs. 0.49 [0.49–0.49] g/L; P=0.011) than did the non-VPS group (n=21). Intracranial pressure decreased from 400 (375–450) to 164 (145–172) mmH2O in the VPS group (log-rank, P<0.001). The 24-week cumulative survival rates in the VPS and non-VPS groups were 100.0% and 79.8%, respectively (P=0.035). The misdiagnosis rates of tuberculous meningitis were 28.6% and 0.0%, respectively (P=0.008). Conclusions: Ventriculoperitoneal shunting decreased the intracranial pressure and 24-week mortality in patients with human immunodeficiency virus-associated cryptococcal meningitis, but significantly increased cerebrospinal fluid protein levels, leading to a higher misdiagnosis rate of tuberculous meningitis. Physicians should be aware of these changes in the cerebrospinal fluid profiles of patients with human immunodeficiency virus-associated cryptococcal meningitis with a ventriculoperitoneal shunt.
Robust Design of a Multirotor Aerial Vehicle
Authors: Dutta Abhishek 
Published: August 2021

Abstract
This paper introduces the methodology of systematic robust design of a multirotor vehicle as an example of how to carry out the robust design of a physical system. Robustness in aerial vehicles is highly desirable as it guarantees a desired level of performance even under environmental uncertainties. Thus far, robustness has been considered in terms of active control in the space of multirotor vehicles, but exploration of the design space itself is lacking. In this work, a conceptual design followed by a robust design is performed to come up with the specifications that lead to least uncertain performance of the multirotor vehicle with respect to stochastic wind disturbances.
Effect of Ultrasonic Compound Cutting on the Microstructure and Properties of Formed Chip
Authors: Sun Haimeng , Jiao Feng , Niu Ying , Wang Zhuangfei 
Published: August 2021

Abstract
Sever plastic deformation (SPD) with high strain rate can increase the material dislocation density, reduce the grain size and improve the mechanical properties. In this paper, ultrasonic compound cutting (UCC) was proposed to improve the efficiency of preparing ultra-fine grained (UFG) pure copper by sever plastic deformation methods. The motion characteristics and strain rate model of UCC were analyzed, and it was concluded that ultrasonic vibration can increase the strain rate in the primary shear zone. According to the 3D FEM equivalent model of UCC, the UCC and traditional compound cutting (TCC) were compared and analyzed from the perspective of strain rate. The simulation results show that ultrasonic vibration can significantly increase the strain rate of chip. The microstructure and mechanical properties of pure copper chip were studied by using a self-developed machining device. The experiment results show that the grain refinement, dislocation density and micro-hardness of pure copper chip were significantly improved in UCC. When the ultrasonic amplitude is 3 µm, the UCC chip grains are about 2.66 µm and the hardness reaches 124 HV, which is about 8% higher than the hardness of TCC chip. The findings of this research provide an important reference for machining UFG pure copper with enhanced mechanical properties.
The Protective Role of Protocol Biopsy for Allograft Kidney Maintenance in Kidney Transplantation
Authors: Lee Okjoo , Lee Kyo Won , Park Jae Berm , Lee Jung Eun , Hwang Na Young , Kim Kyunga 
Published: August 2021

Abstract
Many studies have reported that protocol biopsy (PB) may help preserve kidney function in kidney transplant recipients. Early detection and treatment of subclinical rejection may reduce the incidence of chronic allograft nephropathy and graft failure. However, no consensus has been reached regarding PB effectiveness, timing, and policy. This study aimed to evaluate the protective role of routine PB performed 2 weeks and 1 year after kidney transplantation. We reviewed 854 kidney transplant recipients at the Samsung Medical Center between July 2007 and August 2017, with PBs planned at 2 weeks and 1 year after transplantation. We compared the trends in graft function, chronic kidney disease progression, new-onset chronic kidney disease, infection, and patient and graft survival between the 504 patients who underwent PB and 350 who did not undergo PB. The PB group was again divided into two groups: the single PB group (n = 207) and the double PB group (n = 297). In the PB group, the donors and recipients were significantly older and there was a greater presence of recipient diabetes mellitus and donor hypertension, donor-specific antigen, and a higher proportion of ABO-incompatible kidney transplantations. The PB group was significantly different from the no-PB group in terms of the trends in graft function (estimated glomerular filtration rate). The Kaplan-Meier curve showed that PB did not significantly improve graft survival or overall patient survival. However, in the multivariate Cox analysis, the double PB group had advantages in graft survival, chronic kidney disease progression, and new-onset chronic kidney disease. PB can play a protective role in the maintenance of kidney grafts in kidney transplant recipients.
Overlapped Gate-Source/Drain H-shaped TFET: Proposal, Design and Linearity Analysis
Authors: Upadhyay Utkarsh , Raman Ashish , RANJAN RAVI , Kumar Naveen 
Published: August 2021

Abstract
In this paper, the proposed design of H-shaped TFET has been discussed. This design is providing a high Ion/Ioff ratio with better Ion. HfO2 is used for better tunneling current. With this device, Different parameters such as unit parameter, analogue parameter, and linearity parameter have been studied and investigated the output of the H-TFET. As unit parameters, the electric field, electric potential, energy band diagram, and non-local band-to-band tunneling rate (BTBT) have all been observed. Second and third-order harmonics distortion (HD2, HD3), third-order current intercept point (IIP3), third-order intermodulation distortions (IMD3), and second and third-order voltage intercept point (VIP2, VIP3) are evaluated as linearity parameters that characterize the device's distortions and linearity. We obtained Ion\({\text{=1.6×}10}^{-4}\) A/µm, Ioff=2.1\({\text{×}10}^{-19}\) A/µm, Ion /Ioff=7.6\({\text{×}10}^{14}\),threshold voltage Vt=0.3449 V. © 2017 Elsevier Inc. All rights reserved.
Impact of treatment with anti-parasitic (Ivermectin) during prepartum period on mastitis and postpartum reproductive efficiency measurements in Egyptian Baladi cows
Authors: Damarany Ahmed Ismail 
Published: August 2021

Abstract
The current investigation was designed to determine the effect of treatment with anti-parasites (Ivermectin) on mastitis and postpartum reproductive efficiency measurements in Egyptian Baladi cows. Twenty-four of the cows included in this study were split into two groups. The first group (n = 12 cows) was injected two months prepartum with two doses (15 days inter-treatment) of IVOMEC® Plus solution subcutaneously behind the shoulder, 1ml/50kg live body weight as prescribed by the manufacturer, whilst the other group (n = 12 cows) was used as a control group. The present results revealed that the incident rate of mastitis in treated cows’ group was significantly (P < 0.05) lower (16.7%) than control cows’ group (41.7%). The conception rate in treated cows’ group was significantly (P < 0.05) higher (66.7%) compared with (33.3%) in control group. The time from calving to conception in treated cows was significantly (P < 0.05) lower 86.5 ± 11.4 days compared to 123.3 ± 13.2 days in the control group. The occurrence rate of anestrous cases was significantly (P < 0.05) lower (8.3%) in treated cows than (control group) (25%). In conclusion, the current study indicates there is an association between treated cows with anti-parasites (Ivermectin) two months prepartum and incidence rate of mastitis addition an improvement in the reproductive performance in treated cows relative to untreated cows. Therefore, the current study recommends the herdsmen treats their cows with anti-parasites (Ivermectin) during the prepartum period for less mastitis incidence rate and better reproductive efficiency.
Novel Method for Evaluating Spatial Resolution of Magnetic Resonance Images
Authors: takeuchi tomokazu , Hayashi Norio , Asai Yuta , Kayaoka Yuka , Yoshida Kiichi 
Published: August 2021

Abstract
Purpose Recently, several methods for evaluating the spatial resolution of magnetic resonance imaging (MRI) have been reported. However, these methods are not simple and can only be used for specific devices. The International Electrotechnical Commission (IEC) 62464-1 recommends a method that uses a periodic array pattern to evaluate the spatial resolution of an MRI device. In this study, we develop a new method (the ladder method) and evaluate its measurement accuracy by adapting the IEC method to evaluate the spatial resolution. Methods First, the adaptation of the IEC method is analyzed by simulating the ladder method using a phantom with a periodic pattern, which is constructed using acrylic plates and a nickel sulfate aqueous solution. Subsequently, the ladder method is evaluated in terms of spatial resolution by dividing the standard deviation (SD) by the average signal in the region of interest (ROI) on the ladder phantom image. To evaluate the precision of the ladder method, it is compared with the modulation transfer function (MTF) using a magnitude image with the partial volume effect of the edge image. Results The simulation result shows that the evaluation of the spatial resolution using the ladder method is viable, in which a coefficient of correlation of 0.90 or higher is obtained for all evaluations using the ladder and MTF methods. ConclusionsThe ladder method can be assessed using only the signal mean value and SD in the ROI on the target image. Therefore, the ladder method is a promising method as a substitute for the MTF.