bluuebunny/crossref_metadata_2025_split huggingface dataset:
Investigating the Preventive Effects of 99Tc-Methylenediphosphonate on a Glucocorticoid-Induced Osteoporosis Rabbit Model
Authors: Li Ying , Cai Min , Zhang Ruihuan , Wang Jun , Zhu Huijing , Wang Chunhua , Min Weiqi , Chen Yiheng , Yan Li 
Published: August 2021

Background and Objective:
Osteoporosis is a worldwide healthcare challenge. Conventional
medications for osteoporosis prevention are not clinically effective or associated with gastrointestinal
tract adverse effects. The present study aimed to comparatively investigate the effects
of technetium-99 conjugated with methylene diphosphonate (99Tc-MDP) and calcium carbonate
and alendronate on the prevention and treatment of osteoporosis in glucocorticoid-induced osteoporosis
rabbit model through evaluating bone alkaline phosphatase (B-ALP), TRAP-5b levels and
histopathological parameters.

Methods:
Forty healthy female New Zealand rabbits were randomly divided into five groups (each
n=8), including control group (Control Group), osteoporosis model group (GIO Group), osteoporosis
model + 99Tc-MDP group (99Tc-MDP Group), osteoporosis model + alendronate group (Alendronate
Group), and osteoporosis model + calcium carbonate group (calcium carbonate Group). Animals
in each group were treated with corresponding interventions for 14 weeks. The blood samples
were collected at the first and 14th week, and B-ALP and TRAP-5b levels were detected by enzyme-
linked immunosorbent assay (ELISA). The rabbits were anesthetized at the 14th week, and
pathological cytological observation was performed on both femurs.

Results:
The age and weights of rabbits in different groups had no statistically significant differences
(P>0.05). B-ALP levels in serum of all groups except for the Control Group decreased after
treatment, but the differences were not statistically significant (P>0.05). TRAP-5b levels in serum
of all groups increased after treatment. Specifically, differences in the GIO Group and calcium carbonate
group were statistically significant (P<0.05), while differences in 99Tc-MDP Group and alendronate
Group were not statistically significant (P<0.05). Pathological sections revealed that the
control group presented normal bone tissue morphology. The bone tissue morphology of the 99Tc-
MDP group and alendronate group was similar to control group and GIO group. Moreover, the calcium
carbonate group and GIO group exhibited similar bone tissue morphology.

Conclusions:
99Tc-MDP has a preventive effect on the glucocorticoid-induced osteoporotic rabbit
model. This osteoporosis preventive effect might be attributed to the capacities of 99Tc-MDP in promoting
the osteoblasts generation and inhibiting the generation and reducing the activity of osteoclasts.
Sequence Analysis, Structure Prediction of Receptor Proteins and In Silico Study of Potential Inhibitors for Management of Life Threatening COVID-19
Authors: Chatterjee Abhik , Basak Hriday Kumar , Saha Soumen , Ghosh Joydeep , Paswan Uttam , Karmakar Sujoy , Pal Ayon 
Published: August 2021

Background:Treatment of the Covid-19 pandemic caused by the highly contagious and pathogenic SARS-CoV-2 is a global menace. Day by day, this pandemic is getting worse. Doctors, scientists and researchers across the world are urgently scrambling for a cure for novel corona virus and continuously working at break neck speed to develop vaccines or drugs. But to date, there are no specific drugs or vaccines available in the market to cope up with the virus.Objective:The present study helps us to elucidate 3D structures of SARS-CoV-2 proteins and also to identify natural compounds as potential inhibitors against COVID-19.Methods:The 3D structures of the proteins were constructed using Modeller 9.16 modeling tool. Modelled proteins were validated with PROCHECK by Ramachandran plot analysis. In this study, a small library of natural compounds (fifty compounds) was docked to the hACE2 binding site of the modelled surface glycoprotein of SARS-CoV-2 using AutoDock Vina to repurpose these inhibitors against SARS-CoV-2. Conceptual density functional theory calculations of the best eight compounds had been performed by Gaussian-09. Geometry optimizations for these molecules were done at M06-2X/ def2-TZVP level of theory. ADME parameters, pharmacokinetic properties and drug likeness of the compounds were analyzed using swissADME website.Results:In this study, we analysed the sequences of surface glycoprotein, nucleocapsid phosphoprotein and envelope protein obtained from different parts of the globe. We modelled all the different sequences of surface glycoprotein and envelop protein in order to derive 3D structure of a molecular target, which is essential for the development of therapeutics. Different electronic properties of the inhibitors have been calculated using DFT through M06-2X functional with def2-TZVP basis set. Docking result at the hACE2 binding site of all modelled surface glycoproteins of SARSCoV- 2 showed that all the eight inhibitors (actinomycin D, avellanin C, ichangin, kanglemycin A, obacunone, ursolic acid, ansamiotocin P-3 and isomitomycin A) studied here were many folds better compared to hydroxychloroquine which has been found to be effective to treat patients suffering from COVID-19. All the inhibitors meet most of the criteria of drug likeness assessment.Conclusion:We expect that eight compounds (actinomycin D, avellanin C, ichangin, kanglemycin A, obacunone, ursolic acid, ansamiotocin P-3 and isomitomycin A) can be used as potential inhibitors against SARS-CoV-2.
Mesenchymal Stem Cells in Veterinary Regenerative Therapy: Basic Physiology
and Clinical Applications
Authors: Chandra Vikash , Mankuzhy Pratheesh , Sharma G. Taru 
Published: August 2021

Background:
The consistent, self-renewal capability and wide-ranged differentiation potential
during specific physiologic conditions mark stem cells as a novel candidate not only for
biomedical research and regenerative therapy but also as an alternative source in research related to
life sciences. This vital and distinct characteristic of stem cells enables them to offer unprecedented
hope in treating many diseases and disorders, which are otherwise difficult to treat. Several efforts
are still being undertaken to enhance the efficiency of MSCs for better therapeutic applications.

Objective:
In the recent past, several studies have been conducted regarding the isolation of stem
cells from diverse sources and are being used clinically in veterinary regenerative therapy. However,
to date, only a few systemic studies are available. This study provides a comprehensive analysis
of the findings from basic and applied research conducted on stem cell therapeutics with particular
emphasis on animals.

Result:
On the basis of their sources, stem cells can be classified as adult or embryonic stem (ES)
cells. Physiologically, the ES cells have the capability to differentiate into all body cells and develop
into the normal adult organism, whereas adult stem cells serve as a repair system by restoring damaged
tissues of the body. The adult stem cells referred to as Mesenchymal stem cells (MSCs) can
be derived from various adult body organs, whereas embryos give rise to embryonic stem cells.
MSCs possess the unique property of proliferation, trans-differentiation, and secretion of important
biomolecules to create a microenvironment, which is immunosuppressive and stimulates native
MSCs of damaged tissue. MSCs being immunocompromised cells can be used in autologous as
well as in allogenic mode.

Conclusion:
In veterinary therapeutics, MSCs equipped with engineering and pharmaceutical modifications
are offered as potential candidates in the treatment of wound healing, nerve injury,
bone/ligament injury, etc., and also bear a great hope for the improvement of udder health and milk
production in animals.
Predictors of Booster Engagement Following a Web-Based Brief Intervention for Alcohol Misuse Among National Guard Members: Secondary Analysis of a Randomized Controlled Trial
Authors: Coughlin Lara N , Blow Frederic C , Walton Maureen , Ignacio Rosalinda V , Walters Heather , Massey Lynn , Barry Kristen L , McCormick Richard 
Published: August 2021

Background
Alcohol misuse is a major health concern among military members. Reserve component members face unique barriers as they live off base with limited access to behavioral health services. Web and app-based brief interventions are a promising means to improve access to treatment for those who misuse alcohol, with the use of booster sessions to enhance effectiveness, solidify gains, and reinforce changes. However, little is known about who will engage in booster sessions.

Objective
This study aims to evaluate booster engagement across booster delivery modalities (Web and Peer) and identify participant-specific factors associated with booster session engagement.

Methods
Following a brief web-based alcohol misuse intervention in National Guard members (N=739), we examined engagement in a series of three booster sessions. Using unadjusted and adjusted models, demographic and clinical characteristics that may serve as predictors of booster session engagement were examined across the 2 arms of the trial with different types of booster sessions: peer-delivered (N=245) and web-delivered (N=246).

Results
Booster session completion was greater for Peer than Web Booster sessions, with 142 (58%) service members in the Peer Booster arm completing all three boosters compared with only 108 (44%) of participants in the Web Booster arm (χ23=10.3; P=.006). In a model in which the 2 groups were combined, socioeconomic factors predicted booster engagement. In separate models, the demographic and clinical predictors of booster engagement varied between the 2 delivery modalities.

Conclusions
The use of peer-delivered boosters, especially among subsets of reserve members at risk of lack of engagement, may foster greater uptake and improve treatment outcomes.

Trial Registration
ClinicalTrials.gov NCT02181283; https://clinicaltrials.gov/ct2/show/NCT02181283
Adapting to the Pandemic: Protocol of a Web-Based Perinatal Health Study to Improve Maternal and Infant Outcomes
Authors: Tzilos Wernette Golfo , Countryman Kristina , Mmeje Okeoma , Ngo Quyen M , Zlotnick Caron 
Published: August 2021

Background
The identification of interconnected health risks during the perinatal period offers an opportunity to prevent negative maternal and infant health outcomes. Marijuana, opioid, and other substance use during pregnancy is a rapidly growing public health concern with significant and costly health consequences for the woman and the developing fetus. Pregnant persons who misuse substances are disproportionately more likely to engage in risky sexual behaviors resulting in sexually transmitted infections (STIs), which are on the rise in this population and can lead to adverse effects on maternal health and on fetal development.

Objective
Our goal is to continue testing an innovative and low-cost technology-delivered intervention, the Health Check-Up for Expectant Moms (HCEM), which simultaneously targets alcohol and drug use and STI risk during pregnancy, both of which are on the rise during the COVID-19 pandemic.

Methods
We describe the ways in which we have adapted the web-based HCEM intervention to continue recruitment and study enrollment during the pandemic.

Results
Study recruitment, visits, and participant safety assessments were all successfully modified during the initial year of the COVID-19 pandemic. Compared to in-person recruitment that occurred prepandemic, remote recruitment yielded a greater proportion of women enrolled in the study (83/136, 61.0% vs 43/52, 83%) in a shorter period (12 months vs 7 months).

Conclusions
Despite study challenges related to the pandemic, including time and effort adapting to a remote protocol, remote recruitment and visits for this study were found to constitute a successful approach.

Trial Registration
ClinicalTrials.gov NCT03826342; https://clinicaltrials.gov/ct2/show/NCT03826342

International Registered Report Identifier (IRRID)
DERR1-10.2196/30367
Keragaman, Dominasi Tikus Silvatik, Kepadatan Pinjal dan Kewaspadaan Pes di Daerah Fokus Pes di Kecamatan Cepogo Kabupaten Boyolali
Authors: Raharjo Jarohman , Wijayanti Tri 
Published: August 2021

One of rodent borne diseases that exist in Indonesia is pes with flea as vector and rat as reservoir. The objective of this research were to find out the diversity, dominance of rats, the density of rat and fleas as monitoring efforts for vigilance of plague transmission. The study was conducted in September-October 2016 in Wonodoyo and Kembang Kuning village, Cepogo Sub District of Boyolali District. The research is descriptive with cross sectional approach. Samples collection were conducted by rats survey used single live trap in the silvatic area. The results showed that rats trap success in Wonodoyo village was 3.8% (38 rats) and 1,1% (11 rats) in Kembang Kuning village. Four kind of silvatic rats were Rattus exulans, R. tiomanicus, Niviventer fulvescens and Maxomys surifer. Most of the rats caught (73.68%) in Wonodoyo Village were infested with fleas, while in Kembang Kuning Village it was 27.27%. All of fleas identified as Stivalius cognatus. The specific and general flea index value of S. cognatus in Wonodoyo Village is 2.66; Kembang Kuning Village is 0.45. Early vigilance is very important to do to prevent the transmission of bubonic plague originating from silvatic rats in Cepogo District, particularly in Wonodoyo Village.
Leptospira pada Tikus dan Badan Air serta Riwayat Penularan Penderita di Daerah Baru Kasus Leptospirosis di Bantul
Authors: Sholichah Zumrotus , Wahyudi Bondan Fajar , Sianturi Corry Laura Junita , Astuti Novia Tri 
Published: August 2021

In 2017 leptospirosis cases occur in new areas in Bantul where cases have never been previously reported. Cases still occur in 2018 with an interval of two months after the first case. Preventive and curative efforts in the context of controlling cases have been carried out by the local health office, in addition to these control efforts, it is also necessary to know the presence of Leptospira in rats and water bodies in the area as well as the history of patients transmission. This study was conducted to provide epidemiological data, especially the status of Leptospira in rats as reservoir animal and water bodies as well as a history of patient activity as basic data for leptospirosis control in Gilangharjo Village. This cross-sectional study was conducted at the case location in RT 03 Jodog, Gilangharjo Village, Pandak District, Bantul Regency, which was conducted in April 2018. Leptospira infection status was carried out on rats and water bodies by molecular examination, while the history of transmission based on interviews with patients and the result of the examination of Leptospira status. Rat species caught were dominated by R.tanezumi, R. norvegicus and B. indica. Positive rat infected with Leptospira were found in B. indica while in water bodies Leptospira contamination was not found. Infected rat can be a source of transmission for humans and other animals. Most of the patient's activities were carried out in the fields and at home, all of the patients had wounds that were not treated, and had a history of contact with rats that might transmit Leptospira to humans.
Efektivitas Tablet Bacillus thuringiensis H-14 Isolat Salatiga terhadap Jentik Aedes aegypti pada Dua Sumber Air yang Berbeda
Authors: Wardani Arum Triyas , Wigati RA. , Rahardianingtyas Esti , Wianto Rendro , Nugroho Arief 
Published: August 2021

Dengue Hemorrhagic Fever (DHF) is a vector-borne disease that is still a health problem in Indonesia. One of the DHF control effort is to control the larvae of the mosquito vector using Bacillus thuringensis H-14. The weakness of this metode is its short residual effect. This study aims to determine differences in the effectiveness of B. thuringiensis H-14 tested in two different water sources, namely well water and PDAM water in people's houses and its residual effects in the field. The study used a quasi-​experimental, which include laboratory and field experiments in March to September 2017. The research was carried out by producing B. thuringensis H-14 Salatiga isolates in the form of slow release tablets, testing the efficacy and measuring their effectiveness to Aedes aegypti mosquito larvae in two kinds of water sources. The results showed that the content of B. thuringiensis Salatiga isolate in slow release tablets weighing 125 mg was 5 mg. The efficacy test at laboratory scale showed that the LC50 was 0.436 ppm and the LC90 was 2.440 ppm. The effectiveness test in the field of LC50 (PDAM water) is 0.098 ppm and (well water) is 1.909 ppm, while LC90 (PDAM water) is 0.186 ppm and (well water) is 0.909 ppm. The efficacy assay results showed there was no significant difference in the effect of B. thuringiensis tablet between well water and PDAM water. B. thuringiensis H-14 tablets were effective in controlling Ae. aegypti larvae more than 80% until the 7th day in field testing.
MODALIZAÇÃO COMO RECURSO ENUNCIATIVO: A MARCA DA SUBJETIVIDADE EM TEXTO DISSERTATIVO-ARGUMENTATIVO
Authors: Silva Mariana Lima da , Carneiro Monica Fontenelle 
Published: August 2021

Considerando que o recurso da modalização se configura como uma estratégia do sujeito manobrar o texto de acordo com seus interesses, neste artigo, procurou-se analisar sob o viés da Linguística Enunciativa e Textual, o uso dos modalizadores epistêmicos e deônticos em texto dissertativo-argumentativo. No que se refere à fundamentação teórica, recorreu-se as conceitualizações de Authier-Revuz (2004) e Ducrot (1987) e ainda, aos trabalhos de Coracini (1991), Corbari (2016), Nascimento (2010) e Pinto (1994). Como corpus de análise, utilizamo-nos de uma redação nota mil do Exame Nacional do Ensino Médio (ENEM) do ano de 2018. Sobre a abordagem aqui considerada, valemo-nos de pesquisa qualitativa caráter descritivo- interpretativista, pautada na revisão bibliográfica consultada. Como resultado da análise, verificamos a presença das marcas enunciativa de modalização deôntica e epistêmica, ambas remetendo para a construção subjetiva de quem enuncia por meio da argumentação, o que nos permitiu verificar que o uso da modalização como marca enunciativa revela, no texto dissertativo-argumentativo, a atitude do enunciador de chamar atenção, ressaltar argumentos, avaliar informações, ou seja, seu posicionamento.
EL TRABAJO Y LA ACTIVIDAD INDUSTRIOSA EN EL SALÓN DE CLASES UNIVERSITARIO
Authors: Pinto Daniel Fabián Roca Flores , Flores Maria Jose Batista Pinto 
Published: August 2021

O artigo corresponde uma tese de doutorado, tendo como objeto de estudo o trabalho e atividade do professor universitário, utilizando o método da instrução ao sósia (IAS) como instrumento principal na aproximação da atividade industriosa situada na sala de aula, analisada desde a perspectiva ergológica, no intuito de trazer para a discussão uma experiência docente no âmbito da educação superior peruana, na qual emergem os saberes e valores produzidos por três professores formadores de engenheiros de uma universidade de caráter comunitário, evidenciando o metier no uso do método e suas dificuldades e reformulações produzidas.
Antibiotic Susceptibility Pattern, Molecular Characterization of Virulence Genes among Pseudomonas Aeruginosa Isolated from Burn Patients
Authors: Younus Mustafa D. , Bahjat Omar F. , Rashid Sirwan A. 
Published: August 2021

Abstract
In this research a total of 150 samples were obtained from burn and wound patients admitted to the West Erbil Emergence Hospital during period from September 2020 to January 2021. Through cultural, morphological features, biochemical testing and Vitek’s 2 compact systems, 40 isolates of P. aeruginosa have been identified. P. aeruginosa produced various pigments, including blue / green, and yellow / green. The iso1ates of P. aeruginosa were subjected to 14 different antibiotics. Impenim was the most effective antimicrobial agents against all P. aerugionsa isolates, and most of isolates showed high resistance degree to Ampicillin 100%, Chloramphenicol 100%, amoxicillin-clavulanic acid 100%, Cefotaxime 100% and Penicillin 100% while for Aztreonam 32.5%, Meropenem 42.5%, Tobramycin 45%, Gentamycin 45%, Amikacin 45%, Ciprofloxacillin 62.5%, ceftazidime 67.5, % Tetracycline 80%. All Psudomonas aeruginosa isolates were screened using Multiplex polymerase chain reaction (PCR) to check for the presence of (Pvda, LasB, Protease, exoA, exoT, exoU and plch) on its genomic DNA. The findings have shown that (Pvda was 55%, LasB 75%, Protease 65%, exoA 60%, exoT 75%, exoU 60% and, plch 55%) of isolates harbored these genes as a virulence genes.
The Potential of Vā Part 1: Working in the space between
Authors: Simati-Kumar Benita 
Published: August 2021

This article is part of a series of chapters that composed an exegesis, submitted in fulfilment of the requirements for the degree of Doctor of Philosophy. The series is a narrative of discovery through practice-led research. Each article reveals its purpose and significance that leads into the next series, which then eventuate to that final design proposal.
The exegesis is presented in this format, to break down the components that assisted in practice-led research. Each article can be read and unpacked on its own as a learning tool. The purpose of this edited series is for the exegesis to be more accessible and adaptable creatively to those being introduced to practice-led research.
The Potential of Vā Part 1: Working in the space between, presents the contents and an introduction of the exegesis, it positions the research by placing the present investigation in the context of the writer’s previous work. This chapter outlines the contemporary artistic context in which this thesis exists, it also presents in detail the methods employed in the first phase of the research, and the use of traditional knowledge to form the Lala-Vā model framework.
Solving of Linear Volterra-Fredholm Integral Equations via Modification of Block Pulse Functions
Authors: Ahmad Ayyubi 
Published: August 2021

A computational method based on modification of block pulse functions is proposed for solving numerically the linear Volterra-Fredholm integral equations. We obtain integration operational matrix of modification of block pulse functions on interval [0,T). A modification of block pulse functions and their integration operational matrix can be reduced to a linear upper triangular system. Then, the problem under study is transformed to a system of linear algebraic equations which can be used to obtain an approximate solution of linear Volterra-Fredholm integral equations. Furthermore, the rate of convergence is O(h) and error analysis of the proposed method are investigated. The results show that the approximate solutions have a good of efficiency and accuracy.