bluuebunny/crossref_metadata_2025_split huggingface dataset:
Shifting and reducing breathing disturbance in patients with very severe obstructive sleep apnea by modified Z-palatoplasty with one-layer closure in one-stage multilevel surgery
Authors: Huang Ethan I. , Lin Yu-Ching , Huang Shu-Yi , Lin Chin-Kuo , Lin Chieh-Mo 
Published: April 2021

AbstractVery severe obstructive sleep apnea (OSA) with apnea–hypopnea index (AHI) ≥ 60 events/h differs in several areas from OSA with other severities, including having a low-level daytime partial pressure of oxygen and residual on-CPAP (continuous positive airway pressure) AHIs greater than 20/h. Patients with very severe OSA show narrow retroglossal space and confined framework, which is difficult to be enlarged via conventional Uvulopalatopharyngoplasty (UPPP) surgery, resulting in poor response to non-framework surgeries. Our latest report showed efficacy and efficiency for subjects undergoing modified Z-palatoplasty (ZPP) with one-layer closure in a one-stage multilevel surgery. It is unclear whether and how this procedure could help patients with very severe OSA characterized with confined framework. From Mar. 2015 to May 2018, we enrolled 12 patients with very severe OSA receiving one-stage multi-level surgery with modified ZPP with one-layer closure, CO2 laser partial tongue-base glossectomy, and bilateral septomeatoplasty. Our results show that the surgery reduced AHI from 73.8 ± 10.7 to 30.8 ± 23.2 events/h and achieved a mean AHI reduction of 58.3% (p < 0.001 against 0 reduction or no surgery). The surgery shifted components of the breathing disturbances. It reduced more apnea than hypopnea and might convert some apnea to hypopnea.
Exogenous sex hormones, menstrual and reproductive history, and risk of non-melanoma skin cancer among women: a systematic literature review and meta-analysis
Authors: Caini Saverio , De Angelis Simone Pietro , Corso Federica , Fantini Carolina , Raimondi Sara , Pala Laura , Stanganelli Ignazio , de Giorgi Vincenzo , Gandini Sara 
Published: April 2021

AbstractNon-melanoma skin cancers (NMSC) are more frequent among men, but women (especially those aged < 40 years) have experienced steeper growth in their incidence rates in recent years. Hormonal factors were hypothesized to be playing a role in modulating NMSC risk, but the studies published to date provided conflicting results. We systematically reviewed and meta-analysed the studies focusing on the association between hormone-related characteristics (use of exogenous sex hormones, and aspects of menstrual and reproductive history) and the risk of NMSC among women. We included observational and experimental studies published in PubMed and EMBASE until February 2020. We calculated summary relative risk (SRR) and 95% confidence intervals (CI) by applying random effects models with maximum likelihood estimation, and used the I2statistics to quantify the degree of heterogeneity of risk estimates across studies. Eleven independent studies encompassing a total of over 30,000 NMSC cases were included in quantitative analyses. No evidence of an increased NMSC risk emerged among ever vs. never users of oral contraceptives (SRR 1.13, 95% CI 0.88–1.45) or hormones for menopause (SRR 1.09, 95% CI 0.87–1.37). Likewise, age at menarche or at menopause and parity were not associated with NMSC risk. Heterogeneity across studies was low, and pooled results were comparable between NMSC subtypes. We found no evidence that hormonal factors play a role in the pathogenesis of NMSC among women.
Molecular characterization and transcriptional regulation of two types of H+-pyrophosphatases in the scuticociliate parasite Philasterides dicentrarchi
Authors: Folgueira I. , Lamas J. , Sueiro R. A. , Leiro J. M. 
Published: April 2021

AbstractProton-translocating inorganic pyrophosphatases (H+-PPases) are an ancient family of membrane bound enzymes that couple pyrophosphate (PPi) hydrolysis to H+ translocation across membranes. In this study, we conducted a molecular characterization of two isoenzymes (PdVP1 and PdVP2) located in respectively the alveolar sacs and in the membranes of the intracellular vacuoles of a scuticociliate parasite (Philasterides dicentrarchi) of farmed turbot. We analyzed the genetic expression of the isoenzymes after administration of antiparasitic drugs and after infection in the host. PdVP1 and PdVP2 are encoded by two genes of 2485 and 3069 bp, which respectively contain 3 and 11 exons and express proteins of 746 and 810 aa of molecular mass 78.9 and 87.6 kDa. Topological predictions from isoenzyme sequences indicate the formation of thirteen transmembrane regions (TMRs) for PdVP1 and seventeen TMRs for PdVP2. Protein structure modelling indicated that both isoenzymes are homodimeric, with three Mg2+ binding sites and an additional K+ binding site in PdVP2. The levels of identity and similarity between the isoenzyme sequences are respectively 33.5 and 51.2%. The molecular weights of the native proteins are 158 kDa (PdVP1) and 178 kDa (PdVP2). The isoenzyme sequences are derived from paralogous genes that form a monophyletic grouping with other ciliate species. Genetic expression of the isoenzymes is closely related to the acidification of alveolar sacs (PdVP1) and intracellular vacuoles (PdVP2): antiparasitic drugs inhibit transcription, while infection increases transcription of both isoenzymes. The study findings show that P. dicentrarchi possesses two isoenzymes with H+-PPase activity which are located in acidophilic cell compartment membranes and which are activated during infection in the host and are sensitive to antiparasitic drugs. The findings open the way to using molecular modelling to design drugs for the treatment of scuticociliatosis.
COVID-19 associated mortality and cardiovascular disease outcomes among US women veterans
Authors: Tsai Shirling , Nguyen Hang , Ebrahimi Ramin , Barbosa Monica R. , Ramanan Bala , Heitjan Daniel F. , Hastings Jeffrey L. , Modrall J. Gregory , Jeon-Slaughter Haekyung 
Published: April 2021

AbstractThe burden of COVID-19 has been noted to be disproportionately greater in minority women, a population that is nevertheless still understudied in COVID-19 research. We conducted an observational study to examine COVID-19-associated mortality and cardiovascular disease outcomes after testing (henceforth index) among a racially diverse adult women veteran population. We assembled a retrospective cohort from a Veterans Affairs (VA) national COVID-19 shared data repository, collected between February and August 2020. A case was defined as a woman veteran who tested positive for SARS-COV-2, and a control as a woman veteran who tested negative. We used Kaplan–Meier curves and the Cox proportional hazards model to examine the distribution of time to death and the effects of baseline predictors on mortality risk. We used generalized linear models to examine 60-day cardiovascular disease outcomes. Covariates studied included age, body mass index (BMI), and active smoking status at index, and pre-existing conditions of diabetes, chronic kidney disease (CKD), chronic obstructive pulmonary disease (COPD), and a history of treatment with antiplatelet or anti-thrombotic drug at any time in the 2 years prior to the index date. Women veterans who tested positive for SARS-CoV-2 had 4 times higher mortality risk than women veterans who tested negative (Hazard Ratio 3.8, 95% Confidence Interval CI 2.92 to 4.89) but had lower risk of cardiovascular events (Odds Ratio OR 0.78, 95% CI 0.66 to 0.92) and developing new heart disease conditions within 60 days (OR 0.67, 95% CI 0.58 to 0.77). Older age, obesity (BMI > 30), and prior CVD and COPD conditions were positively associated with increased mortality in 60 days. Despite a higher infection rate among minority women veterans, there was no significant race difference in mortality, cardiovascular events, or onset of heart disease. SARS-CoV-2 infection increased short-term mortality risk among women veterans similarly across race groups. However, there was no evidence of increased cardiovascular disease incidence in 60 days. A longer follow-up of women veterans who tested positive is warranted.
Identification of attention-deficit hyperactivity disorder based on the complexity and symmetricity of pupil diameter
Authors: Nobukawa Sou , Shirama Aya , Takahashi Tetsuya , Takeda Toshinobu , Ohta Haruhisa , Kikuchi Mitsuru , Iwanami Akira , Kato Nobumasa , Toda Shigenobu 
Published: April 2021

AbstractAdult attention-deficit/hyperactivity disorder (ADHD) frequently leads to psychological/social dysfunction if unaddressed. Identifying a reliable biomarker would assist the diagnosis of adult ADHD and ensure that adults with ADHD receive treatment. Pupil diameter can reflect inherent neural activity and deficits of attention or arousal characteristic of ADHD. Furthermore, distinct profiles of the complexity and symmetricity of neural activity are associated with some psychiatric disorders. We hypothesized that analysing the relationship between the size, complexity of temporal patterns, and asymmetricity of pupil diameters will help characterize the nervous systems of adults with ADHD and that an identification method combining these features would ease the diagnosis of adult ADHD. To validate this hypothesis, we evaluated the resting state hippus in adult participants with or without ADHD by examining the pupil diameter and its temporal complexity using sample entropy and the asymmetricity of the left and right pupils using transfer entropy. We found that large pupil diameters and low temporal complexity and symmetry were associated with ADHD. Moreover, the combination of these factors by the classifier enhanced the accuracy of ADHD identification. These findings may contribute to the development of tools to diagnose adult ADHD.
Flavonoids increase melanin production and reduce proliferation, migration and invasion of melanoma cells by blocking endolysosomal/melanosomal TPC2
Authors: Netcharoensirisuk Ponsawan , Abrahamian Carla , Tang Rachel , Chen Cheng-Chang , Rosato Anna Scotto , Beyers Wyatt , Chao Yu-Kai , Filippini Antonio , Di Pietro Santiago , Bartel Karin , Biel Martin , Vollmar Angelika M. , Umehara Kaoru , De-Eknamkul Wanchai , Grimm Christian 
Published: April 2021

AbstractTwo-pore channel 2 (TPC2) resides in endolysosomal membranes but also in lysosome-related organelles such as the melanin producing melanosomes. Gain-of-function polymorphisms in hTPC2 are associated with decreased melanin production and blond hair color. Vice versa genetic ablation of TPC2 increases melanin production. We show here an inverse correlation between melanin production and melanoma proliferation, migration, and invasion due to the dual activity of TPC2 in endolysosomes and melanosomes. Our results are supported by both genetic ablation and pharmacological inhibition of TPC2. Mechanistically, our data show that loss/block of TPC2 results in reduced protein levels of MITF, a major regulator of melanoma progression, but an increased activity of the melanin-generating enzyme tyrosinase. TPC2 inhibition thus provides a twofold benefit in melanoma prevention and treatment by increasing, through interference with tyrosinase activity, the synthesis of UV blocking melanin in melanosomes and by decreasing MITF-driven melanoma progression by increased GSK3β-mediated MITF degradation.
Interpretable survival prediction for colorectal cancer using deep learning
Authors: Wulczyn Ellery , Steiner David F. , Moran Melissa , Plass Markus , Reihs Robert , Tan Fraser , Flament-Auvigne Isabelle , Brown Trissia , Regitnig Peter , Chen Po-Hsuan Cameron , Hegde Narayan , Sadhwani Apaar , MacDonald Robert , Ayalew Benny , Corrado Greg S. , Peng Lily H. , Tse Daniel , Müller Heimo , Xu Zhaoyang , Liu Yun , Stumpe Martin C. , Zatloukal Kurt , Mermel Craig H. 
Published: April 2021

AbstractDeriving interpretable prognostic features from deep-learning-based prognostic histopathology models remains a challenge. In this study, we developed a deep learning system (DLS) for predicting disease-specific survival for stage II and III colorectal cancer using 3652 cases (27,300 slides). When evaluated on two validation datasets containing 1239 cases (9340 slides) and 738 cases (7140 slides), respectively, the DLS achieved a 5-year disease-specific survival AUC of 0.70 (95% CI: 0.66–0.73) and 0.69 (95% CI: 0.64–0.72), and added significant predictive value to a set of nine clinicopathologic features. To interpret the DLS, we explored the ability of different human-interpretable features to explain the variance in DLS scores. We observed that clinicopathologic features such as T-category, N-category, and grade explained a small fraction of the variance in DLS scores (R2 = 18% in both validation sets). Next, we generated human-interpretable histologic features by clustering embeddings from a deep-learning-based image-similarity model and showed that they explained the majority of the variance (R2of 73–80%). Furthermore, the clustering-derived feature most strongly associated with high DLS scores was also highly prognostic in isolation. With a distinct visual appearance (poorly differentiated tumor cell clusters adjacent to adipose tissue), this feature was identified by annotators with 87.0–95.5% accuracy. Our approach can be used to explain predictions from a prognostic deep learning model and uncover potentially-novel prognostic features that can be reliably identified by people for future validation studies.
A collagen glucosyltransferase drives lung adenocarcinoma progression in mice
Authors: Guo Hou-Fu , Bota-Rabassedas Neus , Terajima Masahiko , Leticia Rodriguez B. , Gibbons Don L. , Chen Yulong , Banerjee Priyam , Tsai Chi-Lin , Tan Xiaochao , Liu Xin , Yu Jiang , Tokmina-Roszyk Michal , Stawikowska Roma , Fields Gregg B. , Miller Mitchell D. , Wang Xiaoyan , Lee Juhoon , Dalby Kevin N. , Creighton Chad J. , Phillips George N. , Tainer John A. , Yamauchi Mitsuo , Kurie Jonathan M. 
Published: April 2021

AbstractCancer cells are a major source of enzymes that modify collagen to create a stiff, fibrotic tumor stroma. High collagen lysyl hydroxylase 2 (LH2) expression promotes metastasis and is correlated with shorter survival in lung adenocarcinoma (LUAD) and other tumor types. LH2 hydroxylates lysine (Lys) residues on fibrillar collagen’s amino- and carboxy-terminal telopeptides to create stable collagen cross-links. Here, we show that electrostatic interactions between the LH domain active site and collagen determine the unique telopeptidyl lysyl hydroxylase (tLH) activity of LH2. However, CRISPR/Cas-9-mediated inactivation of tLH activity does not fully recapitulate the inhibitory effect of LH2 knock out on LUAD growth and metastasis in mice, suggesting that LH2 drives LUAD progression, in part, through a tLH-independent mechanism. Protein homology modeling and biochemical studies identify an LH2 isoform (LH2b) that has previously undetected collagen galactosylhydroxylysyl glucosyltransferase (GGT) activity determined by a loop that enhances UDP-glucose-binding in the GLT active site and is encoded by alternatively spliced exon 13 A. CRISPR/Cas-9-mediated deletion of exon 13 A sharply reduces the growth and metastasis of LH2b-expressing LUADs in mice. These findings identify a previously unrecognized collagen GGT activity that drives LUAD progression.
The hierarchy of root branching order determines bacterial composition, microbial carrying capacity and microbial filtering
Authors: King William L. , Yates Caylon F. , Guo Jing , Fleishman Suzanne M. , Trexler Ryan V. , Centinari Michela , Bell Terrence H. , Eissenstat David M. 
Published: April 2021

AbstractFine roots vary dramatically in their functions, which range from resource absorption to within-plant resource transport. These differences should alter resource availability to root-associated microorganisms, yet most root microbiome studies involve fine root homogenization. We hypothesized that microbial filtering would be greatest in the most distal roots. To test this, we sampled roots of six temperate tree species from a 23-year-old common garden planting, separating by branching order. Rhizoplane bacterial composition was characterized with 16S rRNA gene sequencing, while bacterial abundance was determined on a subset of trees through flow cytometry. Root order strongly impacted composition across tree species, with absorptive lower order roots exerting the greatest selective pressure. Microbial carrying capacity was higher in absorptive roots in two of three tested tree species. This study indicates lower order roots as the main point of microbial interaction with fine roots, suggesting that root homogenization could mask microbial recruitment signatures.
The Cognitive-Communication Checklist for Acquired Brain Injury: A Means of Identifying, Recording, and Tracking Communication Impairments
Authors: MacDonald Sheila 
Published: April 2021

Purpose
The communication service needs of individuals with acquired brain injuries (ABIs) are frequently overlooked, leading to delays, denials, or premature discharge from communication interventions. This is particularly true for those with subtle cognitive-communication deficits, which may not be apparent until sufficiently challenged at work, in school, or in the community. The purpose of this study was to evaluate a referral tool that could promote understanding of the broad range of communication impairments that occur following ABI and lead to improved identification and referral.

Method
This study evaluated the Cognitive-Communication Checklist for Acquired Brain Injury (CCCABI) through a survey. The CCCABI is a referral tool that summarizes 45 communication difficulties in 10 areas of cognitive-communication functioning. One hundred sixteen speech-language pathologists, 34 multidisciplinary referral sources, and 41 individuals with lived experience of brain injury were surveyed to evaluate the utility of this referral tool.

Results
The need for such a referral tool was endorsed by 96% of speech-language pathology respondents, 91% of multidisciplinary respondents, and 100% of respondents with lived experience of brain injury. Responses supported the CCCABI as a clear, comprehensive, and accessible tool for education and identification of the communication impairments that can occur after ABI.

Conclusion
The CCCABI is a means of increasing understanding of communication service needs following ABI in a manner that is accessible to individuals, families, program designers, funding sources, administrators, and multidisciplinary referral sources.
S stars and s-process in the Gaia era
Authors: Shetye Shreeya , Van Eck Sophie , Jorissen Alain , Goriely Stephane , Siess Lionel , Van Winckel Hans , Plez Bertrand , Godefroid Michel , Wallerstein George 
Published: April 2021

Context. S stars are late-type giants that are transition objects between M-type stars and carbon stars on the asymptotic giant branch (AGB). They are classified into two types: intrinsic or extrinsic, based on the presence or absence of technetium (Tc). The Tc-rich or intrinsic S stars are thermally pulsing (TP-)AGB stars internally producing s-process elements (including Tc) that are brought to their surface via the third dredge-up (TDU). The Tc-poor or extrinsic S stars gained their s-process overabundances via the accretion of s-process-rich material from an AGB companion that has since turned into a dim white dwarf.
Aims. Our goal is to investigate the evolutionary status of Tc-rich S stars by locating them in a Hertzsprung-Russell (HR) diagram using the results of Gaia Early Data Release 3 (EDR3). We combine the current sample of 13 Tc-rich stars with our previous studies of 10 Tc-rich stars to determine the observational onset of the TDU in the metallicity range [−0.7; 0]. We also compare our abundance determinations with dedicated AGB nucleosynthesis predictions.
Methods. We derived the stellar parameters using an iterative tool that combines HERMES high-resolution spectra, accurate Gaia EDR3 parallaxes, stellar evolution models, and tailored MARCS model atmospheres for S-type stars. Using these stellar parameters, we determined the heavy-element abundances by line synthesis.
Results. In the HR diagram, the intrinsic S stars are located at higher luminosities than the predicted onset of the TDU. These findings are consistent with Tc-rich S stars being genuine TP-AGB stars. The comparison of the derived s-process abundance profiles of our intrinsic S stars with the nucleosynthesis predictions provide an overall good agreement. Stars with highest [s/Fe] tend to have the highest C/O ratios.
Fundamental properties of a selected sample of Ap stars: Inferences from interferometric and asteroseismic constraints
Authors: Deal M. , Cunha M. S. , Keszthelyi Z. , Perraut K. , Holdsworth D. L. 
Published: April 2021

Context. Magnetic fields influence the formation and evolution of stars and impact the observed stellar properties. magnetic A-type stars (Ap stars) are a prime example of this. Access to precise and accurate determinations of their stellar fundamental properties, such as masses and ages, is crucial to understand the origin and evolution of fossil magnetic fields.
Aims. We propose using the radii and luminosities determined from interferometric measurements, in addition to seismic constraints when available, to infer fundamental properties of 14 Ap stars préviously characterised.
Methods. We used a grid-based modelling approach, employing stellar models computed with the CESTAM stellar evolution code, and the parameter search performed with the AIMS optimisation method. The stellar model grid was built using a wide range of initial helium abundances and metallicities in order to avoid any bias originating from the initial chemical composition. The large frequency separations (Δν) of HR 1217 (HD 24712) and α Cir (HD 128898), two rapidly oscillating Ap stars of the sample, were used as seismic constraints.
Results. We inferred the fundamental properties of the 14 stars in the sample. The overall results are consistent within 1σ with previous studies, however, the stellar masses inferred in this study are higher. This trend likely originates from the broader range of chemical compositions considered in this work. We show that the use of Δν in the modelling significantly improves our inferences, allowing us to set reasonable constraints on the initial metallicity which is, otherwise, unconstrained. This gives an indication of the efficiency of atomic diffusion in the atmospheres of roAp stars and opens the possibility of characterising the transport of chemical elements in their interiors.