bluuebunny/crossref_metadata_2025_split huggingface dataset:
Current status of traditional Chinese medicine for the treatment of COVID-19 in China
Authors: Chu Liang , Huang Fang , Zhang Mengdan , Huang Biao , Wang Yigang 
Published: July 2021

AbstractAn ongoing outbreak of severe respiratory illness and pneumonia caused by the severe acute respiratory coronavirus 2 (SARS-CoV-2) commenced in December 2019, and the disease was named as coronavirus disease 2019 (COVID-19). Soon after, scientists identified the characteristics of SARS-CoV-2, including its genome sequence and protein structure. The clinical manifestations of COVID-19 have now been established; and nucleic acid amplification is used for the direct determination of the virus, whereas immunoassays can determine the antibodies against SARS-CoV-2. Clinical trials of several antiviral drugs are ongoing. However, there is still no specific drugs to treat COVID-19. Traditional Chinese medicine (TCM) was used in the treatment of COVID-19 during the early stages of the outbreak in China. Some ancient TCM prescriptions, which were efficacious in the treatment of severe acute respiratory syndrome (SARS) in 2002–03 and the influenza pandemic (H1N1) of 2009, have been improved by experienced TCM practitioners for the treatment of COVID-19 based on their clinical symptoms. These developed new prescriptions include Lianhua Qingwen capsules/granules, Jinhua Qinggan granules and XueBiJing injection, among others. In this review, we have summarized the presenting features of SARS-CoV-2, the clinical characteristics of COVID-19, and the progress in the treatment of COVID-19 using TCMs.
Aesculin based glucosamine-6-phosphate synthase inhibitors as novel preservatives for food and pharmaceutical products: in-silico studies, antioxidant, antimicrobial and preservative efficacy evaluation
Authors: Lather Amit , Sharma Sunil , Khatkar Anurag 
Published: July 2021

Abstract
Background
Presently available chemical based synthetic preservative have emerged with various side effects, so the aspiration of natural and side effect free novel preservative has been greatly increased. As the natural preservative exhibit poor side effect with improved preservative efficacy. The recent development in computational studies leads advancement in drug designing and discovery of novel glucosamine-6-phosphate synthase (G-6-P synthase) inhibition based natural antimicrobial preservatives. Here, selected aesculin derivatives were screened for G-6-P synthase inhibition via docking study and evaluated for antioxidant, antimicrobial, preservative efficacy as well stability study.

Results
Modified aesculin derivatives were designed, synthesized and showed potent G-6-P synthase inhibition with remarkable antimicrobial, antioxidant, preservative efficacy and stability study. The molecular docking with target pdb id 1moq from G-6-P synthase resulted with better dock score and energy for compound 1 as compared to standard drugs streptomycin, ciprofloxacin, ampicillin and fluconazole, that supported the wet lab results. Among the synthesized compounds, the compound 1 possessed good antioxidant activity as compared to standard L-ascorbic acid. The resultant data for antimicrobial activity of aesculin derivatives revealed compound 1 as the most potent antimicrobial compound as compared to the standard drugs streptomycin, ciprofloxacin, ampicillin and fluconazole. While compound 2 showed better antimicrobial activity as compared to streptomycin, ciprofloxacin, ampicillin. The preservative efficacy test for compound 1 in aloe vera juice and white lotion USP has been showed the log CFU/mL values within the prescribed limit of USP standard and results were comparable to standard sodium benzoate, ethyl paraben and propyl paraben. Compound 1 has been found to be within prescribed limit of stability study over six month.

Conclusion
Compound 1 showed the potent G-6-P synthase inhibitory, antioxidant, antimicrobial, preservative efficacy and stability study results as compared to standard drugs taken. The results have found comparable to molecular docking results, and this final compound may be used as new preservatives for food and pharmaceutical products. Moreover, the mechanistic insight into the docking poses was also explored by binding interactions of aesculin derivatives inside the pdb id 1moq. These results also supported the results for novel synthesized G-6-P synthase inhibitors.

Graphical abstract
Comparative population genetics of the invasive mosquito Aedes albopictus and the native mosquito Aedes flavopictus in the Korean peninsula
Authors: Shin Jiyeong , Jung Jongwoo 
Published: July 2021

Abstract
Background
Aedes mosquitoes are important invasive species contributing to the spread of chikungunya, dengue fever, yellow fever, zika virus, and other dangerous vector-borne diseases. Aedes albopictus is native to southeast Asia, with rapid expansion due to human activity, showing a wide distribution in the Korean peninsula. Aedes flavopictus is considered to be native to East Asia, with a broad distribution in the region, including the Korean peninsula. A better understanding of the genetic diversity of these species is critical for establishing strategies for disease prevention and vector control.

Methods
We obtained DNA from 148 specimens of Ae. albopictus and 166 specimens of Ae. flavopictus in Korea, and amplified two mitochondrial genes (COI and ND5) to compare the genetic diversity and structure of the two species.

Results
We obtained a 658-bp sequence of COI and a 423-bp sequence of ND5 from both mosquito species. We found low diversity and a nonsignificant population genetic structure in Ae. albopictus, and high diversity and a nonsignificant structure in Ae. flavopictus for these two mitochondrial genes. Aedes albopictus had fewer haplotypes with respect to the number of individuals, and a slight mismatch distribution was confirmed. By contrast, Ae. flavopictus had a large number of haplotypes compared with the number of individuals, and a large unimodal-type mismatch distribution was confirmed. Although the genetic structure of both species was nonsignificant, Ae. flavopictus exhibited higher genetic diversity than Ae. albopictus.

Conclusions
Aedes albopictus appears to be an introduced species, whereas Ae. flavopictus is endemic to the Korean peninsula, and the difference in genetic diversity between the two species is related to their adaptability and introduction history. Further studies on the genetic structure and diversity of these mosquitos will provide useful data for vector control.
Effects of acute exercise on spontaneous physical activity in mice at different ages
Authors: Quintanilha Ana Carolina Silvares , Benfato Izabelle Dias , Santos Robson Luiz Oliveira , Antunes Hanna Karen Moreira , de Oliveira Camila Aparecida Machado 
Published: July 2021

Abstract
Background
Exercise is often used to obtain a negative energy balance. However, its effects on body weight reduction are usually below expectations. One possible explanation is a reduction in spontaneous physical activity (SPA) after exercise since the increase in energy expenditure caused by the exercise session would be offset by the decrease in SPA and its associated energy cost. Thus, we evaluated the effects of a single bout of moderate exercise at individualized intensity on spontaneous physical activity. The impact of the single bout of exercise was determined in early adulthood and at the transition to middle age.

Methods
Male C57bl/6j (n = 10) mice were evaluated at 4 (4 M) and 9 (9 M) months of age. One week after a treadmill Maximal Exercise Capacity Test (MECT), mice performed a 30-min single bout of exercise at 50 % of the maximal speed reached at MECT. An infrared-based system was used to determine locomotor parameters (SPA and average speed of displacement, ASD) before (basal) and immediately after the single bout of exercise for 48 h (D1, 0-24 h; D2, 24-48 h). Food intake was measured simultaneously. Data were analyzed by GEE and statistical significance was set at p < 0.05.

Results
Basal SPA declined from 4 M to 9 M (p = 0.01), but maximal exercise capacity was similar. At both ages, SPA and ASD decreased significantly (p < 0.0001) on day 1 after exercise. On D2, SPA returned to basal levels but ASD remained lower than basal (p < 0.001). The magnitude (% of basal) of change in SPA and ASD on D1 and D2 was similar at 4 M and 9 M. Food intake did not change at 4 M but decreased on D2 at 9 M.

Conclusions
A single bout of moderate exercise decreases physical activity in the first 24 h and average speed of locomotion in the 48 h following exercise. This compensation is similar from early adulthood to the transition to middle age. The decrease in both the amount and intensity (speed) of SPA may compensate for the increase in energy expenditure induced by exercise, helping to understand the below-than-expected effect of exercise interventions to cause a negative energy balance.
A spatial-epidemiological dataset of subjects infected by SARS-CoV-2 during the first wave of the pandemic in Mashhad, second-most populous city in Iran
Authors: MohammadEbrahimi Shahab , Mohammadi Alireza , Bergquist Robert , Akbarian Mahsan , Arian Mahnaz , Pishgar Elahe , Kiani Behzad 
Published: July 2021

AbstractObjectiveIn March 2020, Iran tackled the first national wave of COVID-19 that was particularly felt in Mashhad, Iran’s second-most populous city. Accordingly, we performed a spatio-temporal study in this city to investigate the epidemiological aspects of the disease in an urban area and now wish to release a comprehensive dataset resulting from this study.Data descriptionThese data include two data files and a help file. Data file 1: “COVID-19_Patients_Data” contains the patient sex and age + time from symptoms onset to hospital admission; hospitalization time; co-morbidities; manifest symptoms; exposure up to 14 days before admission; disease severity; diagnosis (with or without RT-PCR assay); and outcome (recovery vs. death). The data covers 4000 COVID-19 patients diagnosed between 14 Feb 2020 and 11 May 2020 in Khorasan-Razavi Province. Data file 2: “COVID-19_Spatiotemporal_Data” is a digital map of census tract divisions of Mashhad, the capital of the province, and their population by gender along with the number of COVID-19 cases and deaths including the calculated rates per 100,000 persons. This dataset can be a valuable resource for epidemiologists and health policymakers to identify potential risk factors, control and prevent pandemics, and optimally allocate health resources.
Differential DNA methylation by early versus late parenteral nutrition in the PICU: a biological basis for its impact on emotional and behavioral problems documented 4 years later
Authors: Jacobs An , Güiza Fabian , Verlinden Ines , Dulfer Karolijn , Garcia Guerra Gonzalo , Joosten Koen , Verbruggen Sascha C. , Vanhorebeek Ilse , Van den Berghe Greet 
Published: July 2021

Abstract
Background
The PEPaNIC multicenter randomized controlled trial (RCT) has shown that early administration of supplemental parenteral nutrition (early-PN) as compared with withholding PN for 1 week (late-PN) induced long-term internalizing, externalizing and total emotional/behavioral problems in critically ill children, as observed 4 years later. Early-PN was further shown to alter the methylation status of 37 CpG-sites in leukocyte DNA between admission and discharge from the pediatric intensive care unit (PICU). In a preplanned subanalysis of the PEPaNIC trial, we now investigated whether the altered methylation of these CpG-sites could statistically explain the negative impact of early-PN on emotion/behavior documented 4 years after PICU admission.

Results
The combination of DNA methylation data and data on behavior 4 years after PICU admission was available for 403 of the 1440 patients (aged 0–17 years at PICU admission) who were included in the PEPaNIC RCT (192 early-PN and 211 late-PN patients). Mediation analyses with use of bootstrapped multivariable non-linear regression analyses adjusted for baseline risk factors revealed that the adverse alterations by early-PN in methylation of the 37 CpG-sites together statistically explained its harmful impact on internalizing, externalizing and total emotional/behavioral problems. When adding the methylation status of the 37 CpG-sites to the models, the explanatory power improved with a 1.710 to 1.851-fold increase, and the impact of the altered methylation status of the CpG-sites explained the impact of the randomization to early-PN versus late-PN.

Conclusions
Abnormal DNA methylation induced by the early use of PN in the PICU provides a biological basis for its long-term harmful effect on emotion/behavior of critically ill children 4 years after PICU admission.
Trial Registration ClinicalTrials.gov NCT01536275, registered February 17, 2012, https://clinicaltrials.gov/ct2/show/NCT01536275.
SNX27-driven membrane localisation of OTULIN antagonises linear ubiquitination and NF-κB signalling activation
Authors: Shi Ruona , Shi Xue , Qin Dajiang , Tang Shibing , Vermeulen Michiel , Zhang Xiaofei 
Published: July 2021

AbstractBackgroundLinear ubiquitination is a novel type of ubiquitination that plays important physiological roles in signalling pathways such as tumour necrosis factor (TNF) signalling. However, little is known about the regulatory mechanisms of linear ubiquitination, except the well-described enzymatic regulators E3 ligase linear ubiquitin chain assembly complex (LUBAC) and deubiquitinase OTULIN.ResultsPreviously, we identified SNX27, a member of the sorting nexin family protein, as a selective linear ubiquitin chain interactor in mass spectrometry-based ubiquitin interaction screening. Here, we demonstrated that the interaction between the linear ubiquitin chain and SNX27 is mediated by the OTULIN. Furthermore, we found that SNX27 inhibits LUBAC-mediated linear ubiquitin chain formation and TNFα-induced signalling activation. Mechanistic studies showed that, upon TNFα stimulation, OTULIN-SNX27 is localised to membrane-associated TNF receptor complex, where OTULIN deubiquitinates the linear polyubiquitin chain that formed by the LUBAC complex. Significantly, chemical inhibition of SNX27-retromer translocation by cholera toxin inhibits OTULIN membrane localization.ConclusionsIn conclusion, our study demonstrated that SNX27 inhibits TNFα induced NF-κB signalling activation via facilitating OTULIN to localize to TNF receptor complex.
Current insights into the implications of m6A RNA methylation and autophagy interaction in human diseases
Authors: Chen Xuechai , Wang Jianan , Tahir Muhammad , Zhang Fangfang , Ran Yuanyuan , Liu Zongjian , Wang Juan 
Published: July 2021

AbstractAutophagy is a conserved degradation process crucial to maintaining the primary function of cellular and organismal metabolism. Impaired autophagy could develop numerous diseases, including cancer, cardiomyopathy, neurodegenerative disorders, and aging. N6-methyladenosine (m6A) is the most common RNA modification in eukaryotic cells, and the fate of m6A modified transcripts is controlled by m6A RNA binding proteins. m6A modification influences mRNA alternative splicing, stability, translation, and subcellular localization. Intriguingly, recent studies show that m6A RNA methylation could alter the expression of essential autophagy-related (ATG) genes and influence the autophagy function. Thus, both m6A modification and autophagy could play a crucial role in the onset and progression of various human diseases. In this review, we summarize the latest studies describing the impact of m6A modification in autophagy regulation and discuss the role of m6A modification-autophagy axis in different human diseases, including obesity, heart disease, azoospermatism or oligospermatism, intervertebral disc degeneration, and cancer. The comprehensive understanding of the m6A modification and autophagy interplay may help in interpreting their impact on human diseases and may aid in devising future therapeutic strategies.
Application of a modified Inception-v3 model in the dynasty-based classification of ancient murals
Authors: Cao Jianfang , Yan Minmin , Jia Yiming , Tian Xiaodong , Zhang Zibang 
Published: July 2021

AbstractIt is difficult to identify the historical period in which some ancient murals were created because of damage due to artificial and/or natural factors; similarities in content, style, and color among murals; low image resolution; and other reasons. This study proposed a transfer learning-fused Inception-v3 model for dynasty-based classification. First, the model adopted Inception-v3 with frozen fully connected and softmax layers for pretraining over ImageNet. Second, the model fused Inception-v3 with transfer learning for parameter readjustment over small datasets. Third, the corresponding bottleneck files of the mural images were generated, and the deep-level features of the images were extracted. Fourth, the cross-entropy loss function was employed to calculate the loss value at each step of the training, and an algorithm for the adaptive learning rate on the stochastic gradient descent was applied to unify the learning rate. Finally, the updated softmax classifier was utilized for the dynasty-based classification of the images. On the constructed small datasets, the accuracy rate, recall rate, and F1 value of the proposed model were 88.4%, 88.36%, and 88.32%, respectively, which exhibited noticeable increases compared with those of typical deep learning models and modified convolutional neural networks. Comparisons of the classification outcomes for the mural dataset with those for other painting datasets and natural image datasets showed that the proposed model achieved stable classification outcomes with a powerful generalization capacity. The training time of the proposed model was only 0.7 s, and overfitting seldom occurred.
Deterministic cooperative hybrid ring-mesh network coding for big data transmission over lossy channels in 5G networks
Authors: Attar Hani H. , Solyman Ahmad A. A. , Alrosan Ayat , Chakraborty Chinmay , Khosravi Mohammad R. 
Published: July 2021

AbstractWired and wireless communication data is getting bigger and bigger at such a high pace. Accordingly, the big data (BD) communication networks should be developed as quickly as the quick increase in the exchanging data size is. Based on this regard, this paper proposes a wired and wireless protocol that applies cooperation Network coding (CoNC) in a wired ring topology (WRT) to improve exchanging the BD significantly in wireless mesh network (WMN). The paper presents a solution for distributed nodes to deal with big data over 5G by proposing Hybrid Ring-Mesh Protocols (HRMP) that exploit the CoNC technique at distributed nodes. The proposed protocol (X-ORING) deterministically combines the data that is received at a base station (BS), where the BS wirelessly retransmits the combined data to the WMN members, instead of just forwarding them to the WMN members. Moreover, all members of the WMN are connected by wired optical fibre channels in a WRT and directly to the BS. The results show that applying CoNC in the proposed protocols exploits the advantages of the WRP between the WMN members, and consequently, the WMN packet error rate is significantly improved. Moreover, using optical fibre wires between the mesh network members and the BS increases the WMN coverage region considerably, and allows the BS to receive all members' packets correctly. Finally, the results show that applying CoNC on the WRT improves the entire network maintenance and reliability greatly, simply because the proposed HRMP can continue broadcasting even if one of the direct optical fibre goes out of serves, i.e. the fibre link between one of the N member and the BS lost the connectivity.
Sputum colour as a marker for bacteria in acute exacerbations of COPD: protocol for a systematic review and meta-analysis
Authors: Spies Ruan , Potter Matthew , Hollamby Ruan , van der Walt Stefan , Hohlfeld Ameer , Ochodo Eleanor , van Zyl-Smit Richard N. 
Published: July 2021

Abstract
Background
Chronic obstructive pulmonary disease (COPD) is a major cause of years of life lost globally. Acute exacerbations of COPD (AECOPD) drive disease progression, reduce quality of life and are a source of mortality in COPD. Approximately 50% of AECOPD are due to bacterial infections. Diagnosing bacterial infection as the aetiology of AECOPD however remains challenging as investigations are limited by practicality, accuracy and expense. Clinicians have traditionally used sputum colour as a marker of bacterial infection in AECOPD, despite the lack of high-quality evidence for this practice. The aim of this systematic review and meta-analysis is to determine the diagnostic accuracy of sputum colour in the diagnosis of bacterial causes of AECOPD.

Methods
Articles will be searched for in electronic databases (MEDLINE, Google Scholar Scopus, Web of Science, Africa-Wide, CINAHL and Health Source Nursing Academy) and we will conduct a review of citation indexes and the grey literature. Two reviewers will independently conduct study selection, against pre-defined eligibility criteria, data extraction and quality assessment of included articles using the QUADAS-2 tool. We will perform a meta‐analysis using a bivariate logistic regression model with random effects. We will explore heterogeneity through the visual examination of the forest plots of sensitivities and specificities and through the inclusion of possible sources of heterogeneity as covariates in a meta-regression model if sufficient studies are included in the analysis. We also perform a sensitivity analysis to explore the effect of study quality on our findings. The results of this review will be reported according to the Preferred Reporting Items for Systematic Reviews and Meta-analysis statement and will be submitted for peer-review and publication.

Discussion
The findings of this review will assist clinicians in diagnosing the aetiology of AECOPD and may have important implications for decision making in resource-limited settings, as well as for antimicrobial stewardship.

Systematic review registration
PROSPERO CRD42019141498
Common fixed point theorems for several multivalued mappings on proximinal sets in regular modular space
Authors: Faried N. , Abd El-Ghaffar H. , Hamdy S. 
Published: July 2021

AbstractIn this paper, we found a common fixed point for several multivalued mappings on proximinal sets in regular modular metric space. Also, we introduced the notions of conjoint F-proximinal contraction as well as conjoint F-proximinal contraction of Hardy–Rogers-type for several multivalued mappings. Furthermore, we enhanced our results by giving an application in integral equations.