bluuebunny/crossref_metadata_2025_split huggingface dataset:
Detection of archived lamivudine-associated resistance mutations in virologically suppressed, lamivudine-experienced HIV-infected adults by different genotyping techniques (GEN-PRO study)
Authors: Montejano Rocio , Dominguez-Dominguez Lourdes , de Miguel Rosa , Rial-Crestelo David , Esteban-Cantos Andrés , Aranguren-Rivas Paula , García-Álvarez Mónica , Alejos Belén , Bisbal Otilia , Santacreu-Guerrero Mireia , Hernando Asunción , Bermejo-Plaza Laura , Cadiñanos Julen , Mayoral Mario , Castro Juan Miguel , Moreno Victoria , Martin-Carbonero Luz , Rodés Berta , Delgado Rafael , Rubio Rafael , Pulido Federico , Arribas José Ramón 
Published: August 2021

AbstractBackgroundPreviously selected lamivudine resistance-associated mutations (RAMs) may remain archived within the proviral HIV-DNA.ObjectivesTo evaluate the ability of proviral DNA genotyping to detect lamivudine RAMs in HIV-1 virologically suppressed participants; the correlation between Sanger and next generation sequencing (NGS); and predictive factors for detection of lamivudine RAMs in proviral DNA.MethodsCross-sectional study of participants on stable antiretroviral therapy and suppressed for ≥1 year. Analysis of proviral DNA was performed by Sanger sequencing in whole blood and by NGS in PBMCs.ResultsWe analysed samples from 102 subjects (52 with and 50 without lamivudine RAMs in historical plasma RNA-genotypes). Among participants with previous lamivudine resistance, Sanger sequencing detected RAMs in 26.9%. Detection rates significantly increased using NGS: 47.9%, 64.6%, 75% and 87.5% with the 20%, 10%, 5% and 1% thresholds, respectively. As for participants without historical lamivudine resistance, Sanger detected the RAMs in 1/49 (2%), and NGS (5% threshold) in 8/45 (17.8%). Multivariate models fitted to the whole population revealed that having a history of lamivudine resistance was a risk factor for detection of lamivudine RAMs by NGS. Among participants with historical lamivudine resistance, multivariate analysis showed that a longer time since HIV diagnosis was associated with persistence of archived mutations by NGS at thresholds of &gt;10% [OR 1.10 (95% CI: 1.00–1.24)] and &gt;5% [OR 1.16 (95% CI: 1.02–1.32)].ConclusionsProviral DNA Sanger sequencing does not detect the majority of historical lamivudine RAMs. NGS increases the sensitivity of detection at lower thresholds, although the relevance of these minority populations with lamivudine RAMs needs further evaluation.
The Role of Autoantibodies in the Diagnosis of Autoimmune Liver Disease: Lessons Learned from Clinical Practice
Authors: van Beers Joyce J B C , Koek Ger H , Damoiseaux Jan G M C 
Published: August 2021

Abstract

Background
Primary biliary cholangitis (PBC), autoimmune hepatitis (AIH) and primary sclerosing cholangitis (PSC) are autoimmune liver diseases associated with distinct autoantibodies. Diagnosis is based upon clinical, serological, and histopathology findings. The role of autoantibodies in the diagnosis of these autoimmune liver diseases, with the focus on PBC and AIH, will be discussed.

Content
When AIH or PBC is suspected, testing for multiple autoantibodies can be requested. In this mini-review, the different ways in which autoantibodies can be tested (indirect immunofluorescence and antigen-specific tests) in the context of PBC and AIH are discussed, as well as the pitfalls in interpreting the test results.

Summary
For appropriate interpretation of test results, an important prerequisite is that the doctor knows which test is used in the laboratory of choice and that the laboratory specialist is aware of what the doctor wants to test for. Good communication between clinician and laboratory specialist can, therefore, aid in the diagnosis of autoimmune liver diseases.
Differentiation Between Chaenomelis Fructus and its Common Adulterant, Guangpi Mugua
Authors: Lyu Mengying , Liu Yang , Qiu Yixing , Yang Shuai , Yuan Hanwen , Wang Wei 
Published: August 2021

Abstract

Background
The dried fruit of Chaenomeles speciosa, known as Chaenomelis Fructus or Zhoupi Mugua, is a type of traditional Chinese medicine (TCM) that is widely used to treat many diseases. Guangpi Mugua, the dried fruit of the Chaenomeles sinensis, is its most commonly known adulterant.

Objective
To establish a robust approach for the quality control and identification of Chaenomelis Fructus.

Method
Thin-layer chromatography (TLC) was optimized and used to discriminate Chaenomelis Fructus from Guangpi Mugua. High-performance liquid chromatography (HPLC) combined with fingerprint analysis and partial least-squares (PLS) discrimination analysis (DA) was employed to study the chemical differences between Chaenomelis Fructus and Guangpi Mugua. The single standard to determine multi-components (SSDMC) method, with credible precision, repeatability, stability, and durability, was developed for quantitative analysis of the abundant markers.

Results
The developed TLC and HPLC methods were effective in the authentication of Chaenomelis Fructus. Moreover, oleanolic acid, ursolic acid, pomolic acid, corosolic acid, 3-O-acetylpomolic acid, and one unknown compound were identified to be critical markers for the discrimination of Chaenomelis Fructus from Guangpi Mugua.

Conclusion
Adulteration has always been a challenge in the development of TCM. This study presents useful insights that may help solve the problem of adulteration during the preparation of Chaenomelis Fructus.

Highlights
The present study provides a systematic method for the quality control of Chaenomelis Fructus. This is therefore the first step towards solving the problem of adulteration to improve the clinical safety and effectiveness of Chaenomelis Fructus.
The Invasion ofMegachile policaris(Hymenoptera: Megachilidae) to Hawai‘i
Authors: Koch Jonathan Berenguer Uhuad , Tabor Jesse Anjin , Montoya-Aiona Kristina , Eiben Jesse A 
Published: August 2021

AbstractIslands are insular environments that are negatively impacted by invasive species. In Hawai‘i, at least 21 non-native bees have been documented to date, joining the diversity of &gt;9,000 non-native and invasive species to the archipelago. The goal of this study is to describe the persistence, genetic diversity, and natural history of the most recently established bee to Hawai‘i, Megachile policaris Say, 1831 (Hymenoptera: Megachilidae). Contemporary surveys identify that M. policaris is present on at least O‘ahu, Maui, and Hawai‘i Island, with the earliest detection of the species in 2017. Furthermore, repeated surveys and observations by community members support the hypothesis that M. policaris has been established on Hawai‘i Island from 2017 to 2020. DNA sequenced fragments of the cytochrome oxidase I locus identify two distinct haplotypes on Hawai‘i Island, suggesting that at least two founders have colonized the island. In their native range, M. policaris is documented to forage on at least 21 different plant families, which are represented in Hawai‘i. Finally, ensemble species distribution models (SDMs) constructed with four bioclimatic variables and occurrence data from the native range of M. policaris predicts high habitat suitability on the leeward side of islands throughout the archipelago and at high elevation habitats. While many of the observations presented in our study fall within the predicted habitat suitability on Hawai‘i, we also detected the M. policaris on the windward side of Hawai‘i Island suggesting that the SDMs we constructed likely do not capture the bioclimatic niche flexibility of the species.
Lethal Effects of Commercial Kaolin Dust and Silica Aerogel Dust With and Without Botanical Compounds on Horn Fly Eggs, Larvae, Pupae, and Adults in the Laboratory
Authors: Showler Allan T , Harlien Jessica L 
Published: August 2021

AbstractThe horn fly, Haematobia irritans irritans (L.) (Diptera: Muscidae), is an important bloodsucking ectoparasite of cattle throughout much of the world. The fly is mostly controlled using conventional synthetic insecticides but as concerns about resistance increase, alternative tactics have come under heightened scrutiny. Four desiccant dust products: Surround WP, a kaolin clay-based wettable powder; CimeXa, comprised of silica aerogel; Drione, silica aerogel + pyrethrins; and EcoVia, silica aerogel + thyme oil, were assessed for their lethal effects against horn fly eggs, larvae, pupae, and adults, under laboratory conditions. Although Surround WP and CimeXa did not prevent egg hatching and (when mixed with manure substrate) pupal development, the two products were associated with moderate reductions of emerged adults, and with complete adult contact mortality within 6 hr and 24 hr, respectively. Drione and EcoVia eliminated egg hatching, pupal development, and adults within 15 min to 1 hr, respectively, whether the flies were exposed to treated filter paper substrate or exposed by immersion in the dusts. Implications for horn fly control and advantages of inert desiccant dust formulations are discussed.
Small Fiber Neuropathy in Patients with Chronic Pain and a Previous Diagnosis of Multiple Chemical Sensitivity Syndrome
Authors: Fileccia Enrico , Incensi Alex , Ventruto Francesco , Rizzo Giovanni , Galimberti Damiano , Rao Giacomo , Salvi Fabrizio , Liguori Rocco , Donadio Vincenzo 
Published: August 2021

Abstract
Small fiber neuropathy (SFN) is characterized by the involvement of Aδ and C fibers leading to sensory, mainly pain, and/or autonomic symptoms. Multiple chemical sensitivity syndrome (MCS) is an incompletely defined condition characterized by the onset of various symptoms in patients after exposure to several chemical substances. Pain is a common symptom in these patients. In this study, we report the histological and clinical data of a cohort of 21 patients who had been diagnosed as having MCS and who were referred to us with the suspicion of SFN because of chronic pain. All patients underwent neurological clinical examination, (including scales for pain and autonomic disorders), and a skin biopsy. Age-matched healthy subjects were used as controls for the skin biopsies. Nerve conduction studies and serum screening to exclude possible causes of peripheral neuropathy were also performed. Skin biopsies disclosed a somatic SFN in all patients. Although the majority (18 out of 21) of patients also had autonomic symptoms. we found sparing of autonomic innervation in the biopsies. These observations suggest that chronic pain in MCS could be secondary to the presence of somatic SFN, although more data are needed to confirm these observations.
Characterizing Canopy Openness Across Large Forested Landscapes Using Spherical Densiometer and Smartphone Hemispherical Photography
Authors: Beeles Katie L , Tourville Jordon C , Dovciak Martin 
Published: August 2021

AbstractCanopy openness is an important forest characteristic related to understory light environment and productivity. Although many methods exist to estimate canopy openness, comparisons of their performance tend to focus on relatively narrow ranges of canopy conditions and forest types. To address this gap, we compared two popular approaches for estimating canopy openness, traditional spherical densiometer and modern smartphone hemispherical photography, across a large range of canopy conditions (from closed canopy to large gaps) and forest types (from low-elevation broadleaf to high-elevation conifer forests) across four states in the northeastern United States. We took 988 field canopy openness measurements (494 per instrument) and compared them across canopy conditions using linear regression and t-tests. The extensive replication allowed us to quantify differences between the methods that may otherwise go unnoticed. Relative to the densiometer, smartphone photography overestimated low canopy openness (&lt;10%) but it underestimated higher canopy openness (&gt;10%), regardless of forest type.
Pure laparoscopic living donor right hepatectomy (with video)
Authors: Gamez B , Benitez J , Puelma F , Jarufe N 
Published: August 2021

Abstract
Only a few centers in the world perform pure laparoscopic right hepatectomy for adult-to-adult living donor liver transplantation. In this procedure, right lobe donation is mostly preferred in view of the higher graft volume usually needed for the recipient. In the area, laparoscopic surgery has had a minor development given the greater technical difficulty and risks for both donor and preservation of the graft. Nevertheless, last 3 years, the advances in major laparoscopic liver surgery suggest that pure laparoscopic right hepatectomy could be a feasible and safe procedure in appropriately selected donors, and mostly, when is performed by surgeons with significant experience in both laparoscopic liver surgery and liver transplantation with living donor liver grafts. This video shows the surgical technique of a pure laparoscopic right hepatectomy in a 30-year-old female volunteered for living donation to her brother.
‘Time Is Not All Powerful’: The Johannine Eschatological Vision in Eugene Vodolazkin’s Laurus
Authors: Bowlby Ewan 
Published: August 2021

Abstract
Eugene Vodolazkin’s Laurus employs literary ‘distortion’ to capture and convey the eschatological paradoxes of the Fourth Gospel. Having outlined the complexity and contradictions of the Johannine eschatological vision, this article describes how Laurus meets the challenge presented by this vision. Rather than seeking to resolve the tension between vertical and horizontal eschatological dimensions, Vodolazkin reshapes time itself to accommodate both realised and future-oriented eschatologies. This remythologising of time is a distortion that brings the reader closer to the rich imaginative depths of Scripture: a powerful form of resistance to limited, inflexible accounts of the ‘real’.
Impact of binary stars on planet statistics – I. Planet occurrence rates and trends with stellar mass
Authors: Moe Maxwell , Kratter Kaitlin M 
Published: August 2021

ABSTRACT
Close binaries suppress the formation of circumstellar (S-type) planets and therefore significantly bias the inferred planet occurrence rates and statistical trends. After compiling various radial velocity and high-resolution imaging surveys, we determine that binaries with a &lt; 1 au fully suppress S-type planets, binaries with a = 10 au host close planets at 15$_{-12}^{+17}$ per cent the occurrence rate of single stars, and wide binaries with a &gt; 200 au have a negligible effect on close planet formation. We show that F = 43 ± 7 per cent of solar-type primaries in magnitude-limited samples do not host close planets due to suppression by close stellar companions. By removing spectroscopic binaries from their samples, radial velocity surveys for giant planets boost their detection rates by a factor of 1/(1-F) = 1.8 ± 0.2 compared to transiting surveys. This selection bias fully accounts for the discrepancy in hot Jupiter and close Neptune occurrence rates inferred from these two detection methods. Correcting for both planet suppression by close binaries and transit dilution by wide binaries, the occurrence rate of small planets orbiting single G-dwarfs is 2.1 ± 0.3 times larger than the rate inferred from all G-dwarfs in the Kepler survey. Additionally, about half (but not all) of the observed increase in small, short-period planets towards low-mass hosts can be explained by the corresponding decrease in the binary fraction.
Investigating the true nature of three hard X-ray sources
Authors: Molina M , Malizia A , Masetti N , Bassani L , Bazzano A , Bird A J , Fiocchi M , Palazzi E , Ubertini P 
Published: August 2021

ABSTRACT
Many of the new high energy sources discovered both by INTEGRAL/IBIS and Swift/BAT have been characterized thanks to extensive, multiband follow-up campaigns, but there are still objects whose nature remains to be asserted. In this paper, we investigate the true nature of three high energy sources, IGR J12134−6015, IGR J16058−7253, and Swift J2037.2+4151, employing multiwavelength data from the near-infrared to the X-rays. Through Gaia and ESO-VLT measurements and through Swift/XRT X-ray spectral analysis, we re-evaluate the classification for IGR J12134−6015, arguing that the source is a Galactic object and in particular a cataclysmic variable. We were able to confirm, thanks toNuclear Spectroscopic Telescope Array(NuSTAR) observations, that the hard X-ray emission detected by INTEGRAL/IBIS and Swift/BAT from IGR J16058−7253 is coming from two Seyfert 2 galaxies which are both counterparts for this source. Through optical and X-ray spectral analysis of SwiftJ2037.2+4151 we find that this source is likely part of the rare and peculiar class of symbiotic X-ray binaries and displays flux and spectral variability as well as interesting spectral features, such as a blending of several emission lines around the iron line complex.
On asymmetric dark matter constraints from the asteroseismology of a subgiant star
Authors: Rato João , Lopes José , Lopes Ilídio 
Published: August 2021

ABSTRACT
The asteroseismic modelling of solar-like stars has proved to be valuable in constraining dark matter. In this work, we study for the first time the influence of asymmetric dark matter (ADM) in the evolution of a subgiant star (KIC 8228742) by direct comparison with observational data. Both spectroscopic and seismic data are analysed with a new approach to the stellar calibration method, in which DM properties can also be considered as free inputs. In another phase of this study, a calibrated standard stellar model (without DM) is used as the benchmark for DM models. We find that the latter models consistently outperform the former for 10−40 ≤ σSD &lt; 10−38 cm2, hinting that the presence of ADM in stars of this type does not go against observations. Moreover, we show that stellar seismology allows us to suggest exclusion limits that complement the constraints set by direct detection experiments. Different seismic observables are proposed to study DM properties and ΔΠℓ is found to be the most reliable, having the potential to build future DM exclusion diagrams. This new methodology can be a powerful tool in the analysis of the data coming from the next generation of asteroseismic missions.
Two-wind interactions in binaries with two orbiting giant stars
Authors: Castellanos-Ramírez A , Rodríguez-González A , Meliani Z , Rivera-Ortiz P R , Raga A C , Cantó J 
Published: August 2021

ABSTRACT
Some red giant envelopes present spiral structures (seen either in dust-scattered stellar continuum or in molecular line emission), the most striking example probably being AFGL 3068. This object has been modeled (both analytically and numerically) in terms of a wind ejected from a star in orbit around a binary companion. We revisit both analytical models and 3D simulations of a wind from an orbiting red giant star, and extend the numerical simulations to the case of a binary with two red giants with strong winds. We find that most two-wind models on the orbital plane show a ‘double spiral’ structure close to the binary source, and that these two arms merge into a single spiral structure at larger distances. However, for the case of a binary with two identical winds, the two spiral arms are still present at large distances from the binary source. We also find that for models of two (not identical) dynamically important winds, a region close to the orbital plane has material from both winds. Also, an approximately conical region centered on the orbital axis is filled exclusively by the wind with larger momentum rate. These two structures lead to morphologies reminiscent of the so-called ‘hour glass’ planetary nebulae. Finally, we find that increasing wind velocity disparities lead to the formation of clumpy structures along the spiral arms. Observations of ‘clumpy spirals’ are therefore likely to indicate the presence of two strong winds from the stars in the central binary system.
Massive young stellar objects in the Local Group irregular galaxy NGC 6822 identified using machine learning
Authors: Kinson David A , Oliveira Joana M , van Loon Jacco Th 
Published: August 2021

ABSTRACT
We present a supervised machine learning methodology to classify stellar populations in the Local Group dwarf-irregular galaxy NGC 6822. Near-IR colours (J − H, H − K, and J − K), K-band magnitudes and far-IR surface brightness (at 70 and 160 $\mu$m) measured from Spitzer and Herschel images are the features used to train a Probabilistic Random Forest (PRF) classifier. Point-sources are classified into eight target classes: young stellar objects (YSOs), oxygen- and carbon-rich asymptotic giant branch stars, red giant branch and red supergiant stars, active galactic nuclei, massive main-sequence stars, and Galactic foreground stars. The PRF identifies sources with an accuracy of ∼ 90 per cent across all target classes rising to ∼96 per cent for YSOs. We confirm the nature of 125 out of 277 literature YSO candidates with sufficient feature information, and identify 199 new YSOs and candidates. Whilst these are mostly located in known star-forming regions, we have also identified new star formation sites. These YSOs have mass estimates between ∼15 and 50 M⊙, representing the most massive YSO population in NGC 6822. Another 82 out of 277 literature candidates are definitively classified as non-YSOs by the PRF analysis. We characterize the star formation environment by comparing the spatial distribution of YSOs to those of gas and dust using archival images. We also explore the potential of using (unsupervised) t-distributed stochastic neighbour embedding maps for the identification of the same stellar population classified by the PRF.
Infrared dust echoes from neutron star mergers
Authors: Lu Wenbin , McKee Christopher F , Mooley Kunal P 
Published: August 2021

ABSTRACT
A significant fraction of binary neutron star mergers occur in star-forming galaxies where the UV-optical and soft X-ray emission from the relativistic jet may be absorbed by dust and re-emitted at longer wavelengths. We show that, for mergers occurring in gas-rich environment ($n_{\rm H}\gtrsim 0.5\rm \, cm^{-3}$ at a few to tens of pc) and when the viewing angle is less than about 30°, the emission from heated dust should be detectable by James Webb Space Telescope (JWST), with a detection rate of $\sim 1\rm \, yr^{-1}$. The spatial separation between the dust emission and the merger site is a few to 10 milli-arcsecs (for a source distance of 150 Mpc), which may be astrometrically resolved by JWST for sufficiently high signal-noise-ratio detections. Measuring the superluminal apparent speed of the flux centroid directly gives the orbital inclination of the merger, which can be combined with gravitational wave data to measure the Hubble constant. For a line of sight within the jet opening angle, the dust echoes are much brighter and may contaminate the search for kilonova candidates from short gamma-ray bursts, such as the case of GRB 130603B.