bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comprehensive Analysis of Aberrantly Expressed CircRNAs in the Ossification of the Posterior Longitudinal Ligament
Authors: Gao Qi-Chang , Zhang Zhi-Zhuang , Shao Tuo , Tang Wei-Long , Hu Yu-Hang , Li Fu-Chun , Shen Hong-Tao , Gu Jia-Ao , Yu Zhan-Ge 
Published: December 2021

Abstract
Introduction With causing severe neurological deficits, ossification of the posterior longitudinal ligament (OPLL) has attracted attention in mounting numbers. Increasing evidence shows that circRNAs act as a vital regulatory role in the biological process of human disease development. However, the potential mechanism of circRNAs in OPLL was not fully revealed.MethodsIn this study, 3 OPLL patients and 3 controls were selected for Microarray analysis, and the expression profiles of circRNAs were established. TargetScan and Miranda's miRNA target prediction software were used to predict the circRNA-microRNA interaction. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis were used to identify the biological process and key pathways. we used the STRING database to analyze and construct a Protein-Protein Interaction (PPI) network. Several circRNAs were randomly selected for quantitative real-time PCR (qRT-PCR) validation. ResultsMicroarray data profiling indicated that 489 circRNAs (234 up and 255 down) were highly differentially expressed with setting screening criteria (FC ≥ 2.0 and p-value ≤ 0.05). GO enrichment and KEGG pathways analysis revealed some processes and pathways that might influence the progress of OPLL such as osteoclast differentiation, vasopressin-regulated water reabsorption, Wnt, MAPK, VEGF signaling pathway. We have obtained some significant genes such as AKT3, ACVR1, RBPJ, NFATC1, PTK2, SLC8A1. Extended sample verification results were consistent with sequencing data.Conclusions It is the first study for portraying circRNAs expression profiles of OPLL. This provides new ideas for the prevention and alleviation of OPLL. The specific functions and mechanisms of circRNAs in OPLL should be further verified to provide more clinical treatment options for OPLL patients.
Hydrotropic Root Behavior in Water-saving Cultivation: a High-resolution Monitoring Study of Soil Water Dynamics
Authors: Li Qichen , Sugihara Toshiaki , Shibusawa Sakae , Li Minzan 
Published: December 2021

Abstract
PurposeSubsurface irrigation has been confirmed to have high water use efficiency (WUE) due to it irrigating only the crop root zone. This study investigated hydrotropic root behavior when a wet zone was produced around the roots by subsurface irrigation to clarify the dynamics of soil water content in the wet zone caused by water absorption of the growing plant. ResultsWe conducted a feasibility study of a high-resolution soil moisture sensing prototype and gathered data to analyze hydrotropism and plant water absorption activity. We applied signal processing, high pass filtering, and Fast Fourier Transform (FFT) to the acquired high-resolution soil moisture data. The results showed distinct fluctuation of moisture at the boundary area, which indicated plant’s biological rhythm of photosynthetic activities. We also quantified root distribution inside and outside the wet zone and observed the shape of the root system from the cross-section of the wet zone. The results show that hydrotropism restricted most of the roots to the inside of the wet zone. Furthermore, root hydrotropic response is nonuniform for all roots of an individual plant. ConclusionsThe results suggest a new method to study hydrotropic root behavior and plant photosynthetic activities. We assumed a mechanical, push-and-pull model of water dynamics at the wetting front and the root mass accumulated by hydrotropism is an important system parameter. To further evaluate a plant’s hydrotropic performance, it is necessary to use stochastic analysis and/or a non-deterministic approach.
Identification of Ten-lncRNA Signatures Predict Disease-free and Overall Survival for Hepatocellular Carcinoma
Authors: Yang Si-shu , Lu Jiong , Xiong Xian-ze 
Published: December 2021

Abstract
Background: The prognosis of hepatocellular carcinoma (HCC) is bleak though it has been improved over recent years. Early diagnosis could improve the survival. Plenty of researches indicate that long non-coding RNAs (lncRNAs) could play an important role in prognostic prediction of cancer as a kind of biomarker. Results: We identified and validated ten-lncRNAs based signatures to predict disease-free survival (DFS) and overall survival (OS) of HCC respectively from lncRNA expression data of HCC patients in The Cancer Genome Atlas (TCGA) database. Stratified survival analysis showed that the performance of lncRNAs related signatures was better than tumor, node, metastasis(TNM) staging system. Functional enrichment analysis showed that organelle fission and regulation of mRNA metabolic process were significantly enriched in differentially expressed lncRNAs (DElncRNAs). Transcriptional misregulation in cancer and mitogen-activated protein kinase (MAPK) signaling pathway were significantly enriched pathways in the pathway enrichment analysis. Conclusion: we constructed two lncRNAs based signatures which could predict prognosis of HCC more accurate than the traditional ways.
The SARS-CoV-2 Host Cell Membrane Fusion Protein TMPRSS2 is A Tumor Suppressor and its Downregulation Promotes Antitumor Immunity and Immunotherapy Response in Lung Adenocarcinoma
Authors: Liu Zhixian , Zhang Zhilan , Feng Qiushi , Wang Xiao-Sheng 
Published: December 2021

Abstract
TMPRSS2, a key molecule for SARS-CoV-2 invading human host cells, has an association with cancer. However, its association with lung cancer remains unexplored. In five lung adenocarcinoma (LUAD) genomics datasets, we explored associations between TMPRSS2 expression and immune signatures, tumor progression phenotypes, and clinical prognosis in LUAD by the bioinformatics approach. We found that TMPRSS2 expression levels correlated negatively with the enrichment levels of both immune-stimulatory and immune-inhibitory signatures, while they correlated positively with the ratios of immune-stimulatory/immune-inhibitory signatures. It indicated that TMPRSS2 levels had a stronger negative correlation with immune-inhibitory than with immune-stimulatory signatures. TMPRSS2 downregulation correlated with increased proliferation, stemness, genomic instability, tumor progression, and worse survival in LUAD. We further validated that TMPRSS2 was downregulated with tumor progression in the LUAD dataset we collected. In vitro and in vivo experiments verified the association of TMPRSS2 deficiency with increased tumor cell proliferation and invasion and antitumor immunity in LUAD. Moreover, in vivo experiments demonstrated that TMPRSS2-knockdown tumors were more sensitive to BMS-1, an inhibitor of PD-1/PD-L1. In conclusion, TMPRSS2 is a tumor suppressor, while its downregulation is a positive biomarker of immunotherapy in LUAD. Our data provide a link between lung cancer and pneumonia caused by SARS-CoV-2 infection.
A Novel Non-invasive Method Allowing for Discovery of Pathologically Relevant Proteins From Small Airways
Authors: Östling Jörgen , Geest Marleen Van , Olsson Henric K , Dahlen Sven-Erik , Viklund Emilia , Gustafsson Per M. , Mirgorodskaya Ekaterina , Olin Anna-Carin 
Published: December 2021

Abstract
BackgroundThere is a lack of early and precise biomarkers for personalized respiratory medicine. Breath contains an aerosol of droplet particles, which are formed from the epithelial lining fluid when the small airways close and re-open during inhalation succeeding a full expiration. These particles can be collected by impaction using the PExA® method (Particles in Exhaled Air), and are derived from an area of high clinical interest previously difficult to access, making them a potential source of biomarkers reflecting pathological processes in the small airways.Research questionOur aim was to investigate if PExA method is useful for discovery of biomarkers that reflect pathology of small airways.Methods and analysis10 healthy controls and 20 subjects with asthma, of whom 10 with small airway involvement as indicated by a high lung clearance index (LCI ≥2.9 z-score), were examined in a cross-sectional design, using the PExA instrument. The samples were analysed with the SOMAscan proteomics platform (SomaLogic Inc). ResultsTwo hundred-seven proteins were detected in up to 80% of the samples. Nine proteins showed differential abundance in subjects with asthma and high LCI as compared to healthy controls. Two of these were less abundant (ALDOA4, C4), and seven more abundant (FIGF, SERPINA1, CD93, CCL18, F10, IgM, IL1RAP). sRAGE levels were lower in ex-smokers (n=14) than in never smokers (n=16). Gene Ontology (GO) annotation database analyses revealed that the PEx proteome is enriched in extracellular proteins associated with extracellular exosome-vesicles and innate immunity.ConclusionThe applied analytical method was reproducible and allowed identification of pathologically interesting proteins in PEx samples from asthmatic subjects with high LCI. The results suggest that PEx based proteomics is a novel and promising approach to study respiratory diseases with small airway involvement.
Intrasellar Arachnoid Cyst, a Rare Radiological Finding: Report of a Case.
Authors: Rodriguez Alejandro Augusto Ortega , Cardera José Luís Caro , Muñoz Jordi de Manuel-Rimbau 
Published: December 2021

Abstract
Intrasellar arachnoid cysts are uncommon radiological findings, generally incidental and clinically silent. We present the case of 70 year-old female who was treated of meningitis due to cerebrospinal fluid nasal fistulae. She was diagnosed of intrasellar arachnoid cyst and managed conservatively because no neurologic, hormonal, symptomatic either CSF fistulae appeared during follow-up. The origin of intrasellar arachnoid cysts is unclear; although an incomplete diaphragma sellae through basal arachnoid membrane herniates may be a plausible theory. Conservative treatment is the usual option, but if hormonal, visual or intracranial hypertension symptoms appeared, surgery may be the best therapy. This entity should be in the differential diagnosis of cystic sellar lesions with other benign cysts and tumors as craniopharyngioma.
Metabolic Activity Via 18 F-FDG PET/CT is Predictive of Microsatellite Instability Status In Colorectal Cancer
Authors: Song Jinling , Wei Maomao , Wang Xuejuan 
Published: December 2021

Abstract
Purpose: Identification of microsatellite instability high (MSI-H) colorectal cancer (CRC) is crucial for screening patients most likely to benefit from immunotherapy. We aim to investigate whether the metabolic characteristics is related to MSI status and can be used to predict the MSI-H CRC. Methods: A retrospective analysis was conducted on 420 CRC patients who were identified via [18F]fluorodeoxyglucose (18F-FDG) positron emission tomography (PET)/computed tomography(CT) prior to therapy. Maximum standardised uptake (SUVmax), mean standardised uptake (SUVmean), metabolic tumour volume (MTV) and total lesion glycolysis (TLG) of the primary tumour were calculated and compared between MSI-H and microsatellite stability (MSS). Predictive factors of MSI status were selected from metabolic parameters and clinicopathological profiles via a multivariate analysis.Results: Of 420 colorectal cancers, 44 exhibited a high incidence of MSI. Both MTV and TLG were significantly higher in MSI-H group compared with the MSS group (P=0.004 and P=0.010, respectively). Logistic regression analysis indicated that CRC with MSI-H were related to younger age (P=0.013), primary lesion located at right hemi-colon (P<0.001) and larger MTV on PET/CT imaging (P=0.019). MTV more than 32.19 cm3 of colorectal cancer was linked to the presence of MSI (P=0.019). Conclusion: Tumor metabolic burden were higher in MSI-H CRC which may be useful for predicting the MSI status of CRC patient and thus aid in determination of immunotherapy for patients with CRC.
Effects of H19/SAHH/DNMT1 on the Oxidative DNA Damage Related to Benzo[a]Pyrene Exposure
Authors: Fu Ye , Li Xuejing , Pan Baolong , Niu Yingying , Zhang Bin , Zhao Xinyu , Nie Jisheng , Yang Jing 
Published: December 2021

Abstract
The mechanisms that long noncoding RNA (lncRNA) H19 binding to S-adenosylhomocysteine hydrolase (SAHH) interacted with DNA methyltransferase 1 (DNMT1) and then regulated DNA damage caused by PAHs remain unclear. A total of 146 occupational workers in a Chinese coke-oven plant in 2014 were included in the final analyses. We used high performance liquid chromatography mass spectrometry (HPLC-MS) equipped to detect urine biomarkers of PAHs exposure, including 2-hydroxynaphthalene (2-NAP), 2-hydroxyfluorene (2-FLU), 9-hydroxyphenanthrene (9-PHE), 1-hydroxypyrene (1-OHP). The levels of SAM and SAH in plasma were detected by HPLC-ultraviolet. By constructing various BEAS-2B cell models exposed to 16 µM Benzo[a]pyrene (BaP) for 24 h, toxicological parameters reflecting distinct mechanisms were evaluated. We documented that urinary 1-hydroxypyrene (1-OHP) levels were positively associated with blood H19 RNA expression (OR: 1.51, 95% CI: 1.03 - 2.19), but opposite to plasma SAHH activity (OR: 0.63, 95% CI: 0.41 - 0.98) in coke oven workers. Moreover, by constructing various BEAS-2B cell models exposed to Benzo[a]pyrene (BaP), we investigated that H19 binding to SAHH exaggerated DNMT1 expressions and activity. Suppression of H19 enhanced the interaction of SAHH and DNMT1 in BaP-treated cells, decreased OGG1 methylation, reduced oxidative DNA damage and lessened S phase arrest. However, SAHH or DNMT1 single knockdown and SAHH/DNMT1 double knockdown showed the opposite trend. A H19/SAHH/DNMT1 axis was involved in OGG1 methylation, oxidative DNA damage and cell cycle arrest by carcinogen BaP.
Respiratory Viruses Among Ethnic Nicobarese During COVID-19 Pandemic
Authors: Muruganandam Nagarajan , Roy Avijit , Sivanandan Nimisha , Vins Alwin , Beniwal Nisha , Potdar Varsha , Parvez Rehnuma 
Published: December 2021

Abstract
Background: Acute respiratory infections (ARIs) and severe acute respiratory illness (SARI) are public health burdens globally. The percentage of non-SARS CoV-2 respiratory viruses among patients having ARI and SARI who visit Car Nicobar's hospital settings is undocumented. Changes in the epidemiology of other respiratory viruses during COVID19 pandemic is being reported worldwide.Methods: Inpatient and outpatient settings at BJR hospital, Car Nicobar Island, India, were used to conduct prospective monitoring for ARI and SARI among Nicobarese tribal members. The patients with ARI and SARI were enlisted in BJR hospital from June 2019 to May 2021. At the ICMR-NIV in Pune, duplex qRTPCR assays were used to test the presence of respiratory viruses. The prevalence of non- SARS CoV-2 respiratory viruses was measured by comparing here between pandemic and pre-pandemic periods.Results: During the COVID19 pandemic, Influenza A (H3N2) (55.7%), and rhinovirus (12.5%) were predominantly reported non-SARS CoV-2 respiratory viruses while Human metapneumovirus (48.1%) and influenza A (H1N1)pdm09 (18.5%) were most commonly reported in the prepandemic period. This result indicates the altered circulation of non-SARS CoV-2 during pandemic.Conclusions: A considerable proportion of respiratory infection was correlated with respiratory viruses. Prevalence of non-SARS CoV-2 respiratory viruses was high at the time of infection when compared with pre-pandemic period, at Car Nicobar Island. This study enlightened the change in circulation of other respiratory viruses among the indigenous Nicobarese tribes. Clinicians and allied medical staff should be more prudent of these respiratory infections.
Konzeptdialoge als Instrument inklusiver Diagnostik? Erfassung kindlicher Präkonzepte, Vorstellungen und Begründungen im Problemlöseprozess – am Beispiel zum Phänomen Stabilität von Brücken
Authors: Hormann Kathrin , Quittkat Laisa M. , Schomaker Claudia 
Published: December 2021

Im Rahmen des Beitrages werden Ergebnisse aus der Analyse von Interviews mit Kindern auf Basis eines Konzeptdialogs zum Phänomen Stabilität von Brücken vorgestellt. Fokussiert werden dabei die kindlichen Präkonzepte und Vorstellungen zu diesem Phänomen sowie die Begründungen, die die Kinder zur Lösung der Problemstellung Bau einer Brücke aus Papier vornehmen. Anknüpfend an diese Ergebnisse werden – im Sinne einer inklusiven Diagnostik – unterschiedliche Lernvoraussetzungen abgeleitet sowie die sich daraus ergebenden Anknüpfungspunkte und Erweiterungen für einen inklusiven Sachunterricht vorgestellt. Abschließend wird auf Basis der Ergebnisse diskutiert, welche Schlussfolgerungen für die Qualifizierung von Lehrkräften gezogen werden können, um so zu einem differenzierteren Verständnis inklusiver Diagnostik beitragen zu können.

Abstract
As part of the contribution, results from the analysis of interviews with children on the basis of a conceptual dialogue on the phenomenon of bridging stability are presented. The focus is on the childlike preconceptions and ideas about this phenomenon, as well as the reasons the children give to solve the problem of building a bridge out of paper. Based on these results – in the sense of an inclusive diagnosis – different learning prerequisites are derived and the resulting connecting point and extensions for an inclusive Sachunterricht are presented. Finally, on the basis of the results, it is discussed which conclusions can be drawn for the qualification of teachers in order to contribute to a more differentiated understanding of inclusive diagnostics.
Deneysel overyan iskemi/reperfüzyon modelinde iloprostun uzak organ olarak karaciğer dokusu üzerindeki koruyucu etkisi
Authors: DURAN Mehmet , YILMAZ Nezir , ANNAÇ Ebru , DOĞUKAN Mevlüt 
Published: December 2021

Amaç: Bu deneysel çalışmada iloprostun karaciğer dokusu üzerindeki koruyucu etkisini bir iskemi/reperfüzyon (İ/R) sıçan modelinde araştırmak amaçlanmıştır.Yöntem: Toplam 32 adet dişi Sprague–Dawley sıçan, 4 eşit gruba ayrıldı. Grup 1’deki sıçanlarda overyan dokulara herhangi bir cerrahi işlem uygulanmadı. Sırasıyla Grup 2, 3 ve 4’teki overyan dokulara ise 3 saatlik torsiyon, 3 saatlik torsiyon + 3 saatlik detorsiyon, ve 3 saatlik torsiyon + 3 saatlik detorsiyon + 60 dakikalık iloprost infüzyonu uygulandı. Dokulardan 7 μm kalınlıkta kesitler alındı, hematoksilen–eozin, Masson trikrom ve toluidin mavisi ile boyandı. Kesitler üzerinde ışık mikroskobik inceleme yapıldı.Bulgular: Grup 2, 3 ve 4’te Grup 1’e kıyasla sinüzoidal dilatasyon ve vasküler konjesyonda istatistiksel olarak anlamlı artış tespit edildi (p&lt;0,05). Grup 4’te diğer gruplara kıyasla daha fazla sinüzoidal dilatasyon ve vasküler konjesyon görüldü. Yine Grup 2, 3 ve 4’te Grup 1’e kıyasla daha fazla enflamasyon, fibrozis, hemoraji ve daha yüksek mast hücre yoğunluğu tespit edildi. Ancak fark istatistiksel olarak anlamlı değildi (p&gt;0,05). Histopatolojik skorlamada Grup 2 ve 4’te benzer, Grup 3’te ise daha yüksek skorlar gözlendi.Sonuç: İloprostun İ/R hasarına karşı karaciğer dokusu üzerinde koruyucu etkileri olduğu sonucuna varılmıştır.
Educación religiosa y regulaciones del cuerpo
Authors: Maciel María Magdalena 
Published: December 2021

Este trabajo analiza las relaciones entre el discurso pedagógico-religioso de una de las instituciones educativas más importantes orientadas a la formación de niñas y jóvenes en Salta hacia fines del siglo XIX -el Colegio Sagrado Corazón- y su inscripción en los cuerpos de las educandas en vistas a su regulación. Para ello se rastrean los discursos referidos a las mujeres, presentes en diferentes fuentes (reglamentos escolares, manuales, normativas, entre otras). El análisis de las fuentes diálogo con las condiciones de producción de las mimas, por lo que se tendrá en cuenta quiénes lo produjeron; el contexto social, cultural, eclesiástico, económico y político; junto a otros elementos significativos.
A Novel Approach for Designing Electrochemical Aptamer-Based
Biosensor for Ultrasensitive Detection of Zearalenone as a Prevalent
Estrogenic Mycotoxin
Authors: Hassani Shokoufeh , Maghsoudi Armin Salek , Akmal Milad Rezaei , Shoeibi Shahram , Ghadipasha Fatemeh , Mousavi Taraneh , Ganjali Mohammad Reza , Hosseini Rohollah , Abdollahi Mohammad 
Published: December 2021

Background:
Zearalenone is a well-known estrogenic mycotoxin produced by
Fusarium species, a serious threat to the agricultural and food industries worldwide.
Zearalenone, with its known metabolites, is a biomarker of exposure to certain fungi, primarily
through food. It has considerable toxic effects on biological systems due to its carcinogenicity,
mutagenicity, renal toxicity, teratogenicity, and immunotoxicity.

Introduction:
This study aims to design a simple, quick, precise, and cost-effective
method on a biosensor platform to evaluate the low levels of this toxin in foodstuffs and
agricultural products.

Methods:
An aptamer-based electrochemical biosensor was introduced that utilizes
screen-printed gold electrodes instead of conventional electrodes. The electrodeposition
process was employed to develop a gold nanoparticle-modified surface to enhance the
electroactive surface area. Thiolated aptamers were immobilized on the surface of gold
nanoparticles, and subsequently, the blocker and analyte were added to the modified surface.
In the presence of a redox probe, electrochemical characterization of differential
pulse voltammetry, cyclic voltammetry, and electrochemical impedance spectroscopy
were used to investigate the various stages of aptasensor fabrication.

Results:
The proposed aptasensor for zearalenone concentration had a wide linear dynamic
range covering the 0.5 pg/mL to 100 ng/mL with a 0.14 pg/mL detection limit. Moreover,
this aptasensor had high specificity so that a non-specific analyte cannot negatively
affect the selectivity of the aptasensor.

Conclusion:
Overall, due to its simple design, high sensitivity, and fast performance, this
aptasensor showed a high potential for assessing zearalenone in real samples, providing a
clear perspective for designing a portable and cost-effective device.