bluuebunny/crossref_metadata_2025_split huggingface dataset:
Research Capability, Support, and Difficulties as Viewed among Faculty Members of the University of Bohol
Authors: Pilongo Leah Wilfreda RE 
Published: October 2021

Research capability in this study is the potential of individuals or staff in the institution to undertake the rigors of effective, efficient, and high-quality research. This study looked into the challenges that professors may face when conducting research. The study was quantitative in nature employing the survey method. The status of the research capacities of faculty from different departments was described using a standardized survey form. From a total of 296 full-time and part-time faculty of the University in 2017, a random sample of 210 respondents was selected with a +/-3.65% margin of error at a 95% confidence interval. This study protocol passed the review of the University’s Research Ethics Committee. The data were analyzed using frequencies, percentages, weighted means, Chi-square, and ANOVA. The findings revealed that the teacher-respondents were relatively young. The results further yielded that the respondents are Moderately Capable of their research capability regarding technical aspects and the major parts of the research paper. As to the other parts of the research paper, they rated themselves as "Capable," as to referencing; teacher respondents rated themselves as "Moderately Capable." Regarding the level of support for the provision of research facilities and resources, respondents rated "Moderately Supportive." When it comes to the levels of difficulty encountered in research writing, respondents perceived it as "Moderately Difficult." Furthermore, results revealed a significant relationship between respondents’ research capability and their age (, sex (, civil status (, highest educational attainment (, number of years in teaching (, and college assignment (. Respondents' perception of the degree of research support was also significantly associated with their age (, sex (, civil status (, highest educational attainment (, number of years in teaching (, and college assignment (. Finally, there were no significant variances in the respondents’ research capability (F=0.644, p=.853), and level of research assistance (F=0.895, p=.581)when respondents were classified according to their departmental assignment.
A Volatilidade da Arquitetura Comercial sob a influência do comercio online e da sinestesia
Authors: Silva Gabriele Cristina Santos , Carvalho Tatiana Ribeiro de 
Published: October 2021

A presente pesquisa explora a arquitetura comercial e as mudanças ocorridas neste setor devido a pandemia do Covid 19 - que intensificou o comercio online - e a intensificação da arquitetura sinestésica aplicado a arquitetura comercial, como fatores que têm influenciado diretamente na qualidade e configuração dos espaços comerciais. Para melhor conceituar a pesquisa será realizada uma investigação acerca das formas de comércio, desde os primórdios até a atualidade, explorando o advento das tradicionais lojas de rua, shoppings centers assim como conceitos recentes: visual merchandising, lojas conceito, pontos de venda, culminando no comércio online. O objetivo é realizar um percurso histórico- investigativo no sentido de entender, fomentar a discussão e analisar como o setor comercial tem estimulado o público-alvo e atraído novos consumidores diante das atuais mudanças no setor, assim como traçar possíveis cenários futuros/ tendências frente às mudanças pelas quais a sociedade está passando. A pesquisa se desenvolve a partir de pesquisas bibliográficas, bem como pesquisas em artigos, teses, material online para melhor compreensão de conceitos e entendimento temporal das práticas de comércio adotada ao longo do tempo.
Apontamentos sobre o processo de metropolização e virtualização do espaço urbano e seus impactos durante e pós-pandemia
Authors: Araujo Eloisa Carvalho de , Soares Julia Morais , Olgador Mariana da Cunha Lima 
Published: October 2021

A discussão que ora se coloca está apoiada no diálogo entre infraestrutura e cidade, a partir de reflexões sobre a “adesão por plataformas digitais na cidade de Niterói com repercussão na mobilidade urbana e na economia compartilhada”. Considera-se o processo de metropolização e virtualização urbana pelo qual a cidade de Niterói vivencia, e a preocupação com a gestão do território municipal durante e pós-pandemia. Para além da compreensão da relação do espaço virtual e do espaço urbano, ressalta-se, a cidade de Niterói como ambiente urbano metropolitano. Tendo por base reflexiva uma abordagem conceitual-teórica e normativa, o artigo tem com objetivo, apresentar o entendimento sobre o processo de metropolização e virtualização do espaço da cidade e suas implicações, e reflexões, decorrentes de um processo complexo, de dinâmicas sociais e econômicas cujos efeitos sobre o espaço urbano são intensos e exigem uma observação atenta e inovadora. A análise, de caráter exploratório e descritivo, ressaltou associação com os diferentes sentidos do virtual no espaço urbano, metropolitano, e potenciais desafios da tecnologia na mobilidade urbana com impactos a serem observados e considerados no contexto de pandemia do covid-19 e futuro por vir. Os resultados revelaram ser interessante trazer maior empirismo ao estudo e buscar construir uma abordagem perceptiva na expectativa de conhecer modos de vida influenciados pela pandemia, praticados pelos cidadãos niteroienses.
Qualitative and biochemical parameters of ejaculate in patients with chronic prostatitis
Authors: Teryoshin Anatolyi T. , Sosnovskij Igor B. , Ihaev Alimhan B. 
Published: October 2021

According to a number of researchers [1,2,3], chronic prostatitis (CP) causes infertility in 28-37% of cases. Disorders of spermatogenesis in patients with CP can be the cause of male infertility and abnormalities in newborns [3, 4]. To predict the fertilizing ability of spermatozoa, an analysis of their morphological characteristics is carried out using the strict criteria of the norm proposed by T. Kruger et al. [5]. However, the morphological and biochemical characteristics of ejaculate in patients with CP have been insufficiently studied.
Purpose of the study. To study the morphological and biochemical parameters of ejaculate in patients with CP.
Materials and methods. 90 patients with CP were examined at the age from 22 to 45 years (on average 33.8 1.4 years) with CP duration from 1 to 11 years (on average 4.7 1.2 years), with duration of infertility from 2 to 5 years (average 3.8 0.7 years).
Results and discussion. It was found that none of the patients was exposed to hyperthermia, industrial poisons, ionizing radiation, did not take narcotic and hormonal drugs, did not abuse nicotine and alcohol.
Evaluation of developmental competence of Sus scrofa domesticus (L.) oocyte-cumulus complexes after intra- and extraovarian vitrification
Authors: Kuzmina T. I. , Chistyakova I. V. 
Published: October 2021

The aim of the present study was to identify the influence of extra- (EOV) and intraovarian vitrification (IOV) on mitochondrial activity (MA) and chromatin state in porcine oocytes during maturation in vitro. During EOV porcine oocytes were exposed in cryoprotective solutions (CPS): CPS-1 – 0.7 M dimethyl sulfoxide (DMSO)+0.9 M ethylene glycol (EG); CPS-2 – 1.4 M DMSO+1.8 M EG; CPS-3 – 2.8 M DMSO+3.6 M EG+0.65 M trehalose. At IOV the ovarian fragments were exposed in CPS-1 – 7.5 % EG+7.5 % DMSO, then in CPS-2 – 15 % EG, 15 % DMSO and 0.5 M sucrose. Straws with oocytes and ovarian fragments were plunged into LN2 and stored. For devitrification, the EOV oocytes were washed in solutions of 0.25, 0.19 and 0.125 M of trehalose, the IOV – in 0.5 and 0.25 М trehalose. Oocytes were cultured in NCSU-23 medium with 10 % fluid of follicles, follicular walls, hormones. 0.001 % of highly dispersed silica nanoparticles (ICP named after A.A. Chuyko of the NAS of Ukraine) were added to all media. The methods of fertilization and embryo culture are presented in the guidelines developed by us. MA and chromatin state were measured by MitoTracker Orange CMTMRos and the cytogenetic method. Significant differences in the level of oocytes with high-expanded cumulus between control and experimental vitrified groups (81 % versus 59 % and 52 %, respectively, p ≤ 0.001) were observed. The percentage of pyknotic cells in native oocytes was 19 %, EOV or IOV oocytes were 39 % and 49 %, respectively. After culture, the level of matured native oocytes was 86 %, 48 % EOV and 33 % IOV cells finished the maturation (p ≤ 0.001). Differences were also observed in the level of MA between groups treated by EOV and IOV (89.4±7.5 µA and 149.2±11.3 µA, respectively, p ≤ 0.05). For the first time, pre-implantation embryos were obtained from oocytes treated by IOV.
Solusi Numerik Persamaan Gelombang Dua Dimensi Dengan Metode Beda Hingga Skema Eksplisit CTCS
Authors: Chasamah Allifia Nur , Jamhuri Muhammad , Alisah Evawati 
Published: October 2021

Persamaan gelombang dua dimensi merupakan persamaan diferensial parsial yang merupakan representasi dari gelombang permukaan yang dihasilkan oleh aliran air. Tujuan daripada penelitian ini adalah untuk menganalisis solusi numerik persamaan gelombng dua dimensi dengan menggunakan metode beda hingga skema eksplisit CTCS (Center Time Center Space). Ada beberapa langkah untuk menyelesaiakan persamaan ini, diantaranya diskritisasi pada persamaan gelombang dua, diskritisasi terhadap kondisi awal serta kondisi batasnya dimensi dengan metode beda hingga pusat terhadap waktu dan ruang. Langkah selanjutnya adalah menentukan syarat kestabilan dan syarat konsistensi untuki menunjukkan bahwa solusi numerik tersebut mendekati solusi analitik. Setelah diperoleh syarat kestabilan dan konsistensi dari skema yang digunakan maka simulasi dari skema yang digunakan dapat dilakukan. Hasil simulasi menunjukkan bahwa penggunaan metode beda hingga skema eksplisit CTCS pada persaman gelombang dua dimensi stabil dengan syarat tertentu. Dari syarat kekonvergenannya skema eksplisit CTCS pada persamaan gelombang dua dimensi diperoleh order errornya . Selanjutnya untuk penelitian dapat meneliti tentang solusi analitik persamaan gelombang dua dimensi dengan menggunakan kondisi batas Neumann
Web Archiving as a Task of National and Local History Bibliography
Authors: Balatskaya N. M. , Martirosova M. B. 
Published: October 2021

The foreign experience of forming the majority of national web archives (in national libraries) and many accessible local history archives of public and university libraries has been analyzed due to the development of a model and methodology for electronic local history archives for Russian libraries.It is shown that the global activity on preserving network content is divided into national and regional segments and is national and local history in both contents. Its goals and structure fully fit into the classical forms and directions of national/local history bibliography, which turns out to be the key in all processes of selection and bibliographic processing of a new class of sources. For selection, all the criteria of connection with the country/region/territory, the place of production of the resource, its national language and content, characteristic of the national/local history bibliography; the selection itself is carried out by library specialists. The analysis has revealed the reasons why the preparation of the archived online content for use, regardless of the capabilities of automated technologies, in practice also requires the involvement of specialist in bibliography, the use of classical tools and methods of bibliographic description and indexing of content. The issue of choosing objects for archiving (sites, subdirectories, individual documents) is specially considered. The special significance, advantages and problems of treating individual documents are shown. The necessity of forming such web archiving strategies is substantiated, in which “starting points” are separate documents, and sites are evaluated and selected for productivity as their “containers”. Taking into account the global trends and the conditions of Russian libraries, a model of web archiving is proposed, the implementation of which, in the absence of a national repository and modern tools, will allow the beginning of accumulating some necessary experience and valuable network content.
Rapid Detection of Orthopoxviruses
Authors: Poltavchenko A. G. , Ersh A. V. , Filatov P. V. , Ushkalenko N. D. , Yakubitsky S. N. , Sergeev Al. A. , Shcherbakov D. N. 
Published: October 2021

The aim of the study was to develop a sensitive and fast immunochemical test for the detection of orthopoxviruses (OPXV) in the “point of care” format.Materials and methods. The analyses were performed in cultured crude and purifed preparations of vaccinia virus, cowpoxvirus, rabbitpoxvirus and ectromelia virus, as well as in the blood and tissue suspensions of infected mice and rabbits. OPXV-antigen was detected by one-stage and two-stage protocols of dot-immunoassay based on ﬂat protein arrays using rabbit polyclonal antibodies as capture and detection reagents.Results and discussion. The results show that the detection limit of OPXV is inversely related to the degree of their purifcation. The one-stage (rapid) protocol is specifc and allows detecting OPXV in crude culture samples of the virus and in clinical samples in the range of 104–103 PFU/ml within 39 minutes. Rapid dot-immunoassay can be applied to detect or exclude the presence of a viral threat in samples and can be useful in various aspects of biosafety provision. The simplicity of the one-stage protocol, the possibility to visually account the results and easy interpretation of the results allow the rapid test to be used in the “point of care” format.
Biological Properties and Genetic Characteristics of Francisella tularensis Strains Isolated in the Territory of the Rostov Region in 2020
Authors: Tsimbalistova M. V. , Sorokin V. M. , Aronova N. V. , Anisimova A. S. , Pichurina N. L. , Pasyukova N. I. , Selyanskaya N. A. , Vodop’yanov S. O. , Vodop’yanov A. S. , Pisanov R. V. , Pavlovich N. V. , Kovalev E. V. , Noskov A. K. 
Published: October 2021

Objective of the study was to investigate biological properties and genetic characteristics of tularemia agent strains isolated from natural foci of the Rostov Region in 2020.Materials and methods. Field material from natural foci of the Rostov Region was examined by serological, bacteriological, biological, and molecular-genetic methods. Cultural-morphological, biochemical, antigenic and pathogenic properties of isolated cultures were studied. Protein profles were obtained through MALDI-TOF MS using mass spectrometer Autoﬂex speed III Bruker Daltonics and Flex Control of Biotyper software. The genetic characteristics of the strains were determined by VNTR and INDEL typing and SNP analysis.Results and discussion. Six strains of tularemia pathogen were isolated from mouse-like rodents using biological method. The investigation of their biological features and data of PCR analysis and INDEL typing with canonical markers showed that all strains are typical representatives of the Francisella tularensis subsp. holarctica biovar EryR. VNTR typing by six genetic loci revealed that all strains belong to four individual genotypes. The strain isolated in 2020 in the Salsky district was identical to the strain which was isolated in the same area in 1989. Based on the whole genome sequencing of two strains, we established that they are closest to the cultures isolated in Turkey (2009, 2012) and Khanty-Mansiysk (2013) by the studied set of SNP markers. Thus, we found that both identical (or closely related) clones of the tularemia agent and new strains with unique genotypes which previously were not described for the Rostov Region can circulate in natural foci of this region for a long period of time.
Regarding Coinfection With Denge Viruses and Agents of Hemocontact Infections in the Socialist Republic of Vietnam
Authors: Ostankova Yu. V. , Naidenova E. V. , Serikova E. N. , Schemelev A. N. , Valutite D. E. , Zueva E. B. , Huinh Hoang Khanh Thu , Semenov A. V. 
Published: October 2021

According to the WHO, there is an increase in the number of cases of dengue fever worldwide. In many countries, where dengue fever is an endemic disease, blood-borne infections associated with hepatitis B and C viruses and HIV are widespread. The Socialist Republic of Vietnam is an endemic region for these pathogens. The unique epidemiological situation in the country provides an excellent opportunity to study the interaction between Arboviruses, agents of parenteral viral hepatitis B and C, and HIV infection in the body of sick people.The aim of this review was to analyze the literature data on the detection of cases of simultaneous infection with Dengue viruses of 1–4 types and agents of blood-borne infections in sick people in the Socialist Republic of Vietnam. Despite the fact that the simultaneous circulation of these pathogens in the patient's body can affect the pathophysiological mechanisms of the disease development, there were very few works devoted to co-infection with Dengue viruses and HIV, hepatitis C or B viruses, including in the regions adjacent to Vietnam. Therefore, research in this direction is promising for both fundamental science and practical medicine.
Bonding of Cast Iron-Aluminum In Bimetallic Castings By High Pressure Die Casting Process
Authors: Wu Mengwu , Yang Jinpeng , Huang Feng , Hua Lin , Xiong Shoumei 
Published: October 2021

Abstract
A practical bimetallic casting consisting of aluminum matrix and cast iron inserts was manufactured via high pressure die casting (HPDC) process. Different surface treatment methods of the cast iron inserts, including salt membrane plating and electrogalvanizing, were adopted to improve the bonding quality of bimetallic castings. Microstructure characterization on the bonding interface was conducted at different locations of bimetallic castings. Results indicate that compounds with flawless and continuously metallurgical bonding interface can be successfully fabricated by the HPDC process with the zinc rack plating treatment on the surface of cast iron inserts which results in a dense zinc coating with an average thickness of 8 μm. The melt flow speed and heat transition during solidification of the HPDC process are two key factors in determining the bonding integrity of bimetallic castings. With the dissolution and diffusion of the very thin zinc coating during solidification, there is no obvious aggregation of zinc element at the metallurgical bonding interface. Instead, a reaction layer with an irregular tongue-like morphology is formed with an average thickness of approximately 1 μm while it mainly consists of intermetallic phases Al60Cu30Fe10 and Al2FeSi, etc.
Endophytic Fungi of Brunfelsia Uniflora: Isolation, Cryopreservation, and Determination of Enzymatic and Antioxidant Activity
Authors: Marsola Sara Jane , Jorge Lais Freitas , Menequeti Adriano Broges , Berteli Miria Benetati Delgado , Lima Thaís Emanuelle Feijó , Bezerra José Luiz , Lopes Ana Daniela , Gazim Zilda Cristiani , Valle Juliana Silveira , Colauto Nelson Barros , Linde Giani Andrea 
Published: October 2021

Abstract
Brunfelsia uniflora (Pohl.) D. Don (Solanaceae), commonly known as manacá-de-cheiro , is widely distributed in Brazil and used by local indigenous peoples as an antirheumatic, antisyphilitic , depurative, emetic, vermifuge, and purgative agent. Several studies have examined the biological activities and phytochemical profile of Brunfelsia ; however, few have focused on the diversity of endophytic microorganisms that colonize members of the genus. This study aimed to isolate and cryopreserve endophytic fungi from B. uniflora and determine their cellulase, laccase, and antioxidant activities. Endophytic fungi were isolated from B. uniflora stems, cultured on wheat grains, immersed in a 150 g/L aqueous sucrose solution, and cryopreserved at −80°C for 1 and 6 months. Cellulase activity was determined by a qualitative test using carboxymethylcellulose medium and laccase activity by a quantitative test based on the oxidation of 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonate). Prior to antioxidant activity assays, fungi were grown in malt extract broth for production of mycelial biomass. A methanolic extract was prepared for evaluation of DPPH• scavenging activity, FRAP activity, and total phenolic content. A total of 46 endophytic fungal isolates were obtained from B. uniflora stems and classified into 24 groups according to morphological similarities. B. uniflora was shown to harbor a great diversity of endophytic fungi . Mycelial viability was observed after 1 and 6 months of cryopreservation at −80 °C. Fungi exhibited cellulase and laccase activities. Isolate CE23 had the highest laccase activity after 7 days of cultivation. Twelve isolates were found to have low total phenolic contents and DPPH• and FRAP activities.
Clown Care In A Palliative Care Unit: A Useful Paradox? A Preliminary Pilot Study Involving Questionnaires And Text Analysis.
Authors: Aouridi-Héritier Laoridi , Alonzo Sophie , Fabbro-Peray Pascale , Guinamard Marion , Blanchard Sylvie , Sawyers Teresa , Rataboul Pierre 
Published: October 2021

Abstract
Title: The impact of Clown Therapy in a Palliative Care Unit (PCU); a pilot studyBackground: Although several studies on clown therapy have demonstrated benefits for children and the elderly, few studies exist on its impact on quality of life in adult terminal patients.We devised this monocentric, prospective, descriptive pilot study, covering both quantitative and qualitative aspects, to test the hypothesis that “clowning” in the PCU would help to improve the quality of life of patients, and benefit those accompanying them and their carers.Methods: 30 terminal patients [age range 63-79, 19 women, 11 men] from the palliative care unit at Nîmes University Hospital were included in the study from 31/03/2016 to 08/11/2016. All had given written informed consent. 2 clowns visited the patients and their companions, inviting them to take part in 15 to 30-minute improvised role-plays to prepare them for the inevitable separation. Primary outcome measures for the impact of the clowns’ interventions were: The Edmonton Symptom Assessment System evaluating patients’ physical symptoms and the McGill Quality of Life Questionnaire assessing patients’ quality of life. The secondary outcome measure was Alceste textual data analysis software to analyse semi-structured interviews held with patients, their companions and carers. Results: However long the clown’s intervention, the ESAS scores indicated a significant improvement in nausea (p=0.0248) and dyspnea (p=0.0476). Scores for depression (p=0.0160) also showed an improvement. A slight difference in the scores for anxiety (p=0.0900) and drowsiness (p=0.0812) was also observed. 2 days after the clowns’ intervention, the Mc Gill scores revealed a significantly greater physical quality of life (p=0.0044) and a very slight increase in the patients’ overall quality of life (p=0.0807). Results of the lexical analysis with Alceste revealed benefits for all those involved and gave an indication for better patient management at the PCU. Conclusions: This pilot study demonstrating the impact of “clowning” on various physical and mental symptoms in adult patients indicates the benefits of such interventions for patients, their companions and carers. Although the usefulness of clowns for children with life-threatening diseases is clear, the results obtained with our study clearly indicate the benefits of these interventions in adult, end-of-life patients. Further studies would be required to confirm these findings.Date of first registration : 20/11/2015Trial registration: ClinicalTrials.gov ID: NCT02610452This research was registered under the following reference : IDRCB 2015-A00719-40 and approved by the local committee for the protection of persons, CPP Sud-Méditerranée III-Nîmes on November 6th, 2015 under the reference 2015.06.03.