bluuebunny/crossref_metadata_2025_split huggingface dataset:
Inclusion of cancer-associated fibroblasts in drug screening assays to evaluate pancreatic cancer resistance to therapeutic drugs
Authors: Brumskill Sarah , Barrera Lawrence N. , Calcraft Peter , Phillips Caroline , Costello Eithne 
Published: December 2021

AbstractPancreatic ductal adenocarcinoma (PDAC) is characterised by a pro-inflammatory stroma and multi-faceted microenvironment that promotes and maintains tumorigenesis. However, the models used to test new and emerging therapies for PDAC have not increased in complexity to keep pace with our understanding of the human disease. Promising therapies that pass pre-clinical testing often fail in pancreatic cancer clinical trials. The objective of this study was to investigate whether changes in the drug-dosing regimen or the addition of cancer-associated fibroblasts (CAFs) to current existing models can impact the efficacy of chemotherapy drugs used in the clinic. Here, we reveal that gemcitabine and paclitaxel markedly reduce the viability of pancreatic cell lines, but not CAFs, when cultured in 2D. Following the use of an in vitro drug pulsing experiment, PDAC cell lines showed sensitivity to gemcitabine and paclitaxel. However, CAFs were less sensitive to pulsing with gemcitabine compared to their response to paclitaxel. We also identify that a 3D co-culture model of MIA PaCa-2 or PANC-1 with CAFs showed an increased chemoresistance to gemcitabine when compared to standard 2D mono-cultures a difference to paclitaxel which showed no measurable difference between the 2D and 3D models, suggesting a complex interaction between the drug in study and the cell type used. Changes to standard 2D mono-culture-based assays and implementation of 3D co-culture assays lend complexity to established models and could provide tools for identifying therapies that will match clinically the success observed with in vitro models, thereby aiding in the discovery of novel therapies.
Epigenetic integrity of paternal imprints enhances the developmental potential of androgenetic haploid embryonic stem cells
Authors: Zhang Hongling , Li Yuanyuan , Ma Yongjian , Lai Chongping , Yu Qian , Shi Guangyong , Li Jinsong 
Published: December 2021

AbstractThe use of two inhibitors of Mek1/2 and Gsk3β (2i) promotes the generation of mouse diploid and haploid embryonic stem cells (ESCs) from the inner cell mass of biparental and uniparental blastocysts, respectively. However, a system enabling long-term maintenance of imprints in ESCs has proven challenging. Here, we report that the use of a two-step a2i (alternative two inhibitors of Src and Gsk3β, TSa2i) derivation/culture protocol results in the establishment of androgenetic haploid ESCs (AG-haESCs) with stable DNA methylation at paternal DMRs (differentially DNA methylated regions) up to passage 60 that can efficiently support generating mice upon oocyte injection. We also show coexistence of H3K9me3 marks and ZFP57 bindings with intact DMR methylations. Furthermore, we demonstrate that TSa2i-treated AG-haESCs are a heterogeneous cell population regarding paternal DMR methylation. Strikingly, AG-haESCs with late passages display increased paternal-DMR methylations and improved developmental potential compared to early-passage cells, in part through the enhanced proliferation ofH19-DMR hypermethylated cells. Together, we establish AG-haESCs that can long-term maintain paternal imprints.
A geomechanics classification for the rating of railroad subgrade performance
Authors: Gomes Correia António , Ramos Ana 
Published: December 2021

AbstractThe type of subgrade of a railroad foundation is vital to the overall performance of the track structure. With the train speed and tonnage increase, as well as environmental changes, the evaluation and influence of subgrade are even more paramount in the railroad track structure performance. A geomechanics classification for subgrade is proposed coupling the stiffness (resilient modulus) and permanent deformation behaviour evaluated by means of repeated triaxial loading tests. This classification covers from fine- to coarse-grained soils, grouped by UIC and ASTM. For this achievement, we first summarize the main models for estimating resilient modulus and permanent deformation, including the evaluation of their robustness and their sensitivity to mechanical and environmental parameters. This is followed by the procedure required to arrive at the geomechanical classification and rating, as well as a discussion of the influence of environmental factors. This work is the first attempt to obtain a new geomechanical classification that can be a useful tool in the evaluation and modelling of the foundation of railway structures.
Thermal Forcing of the Nocturnal Near Surface Environment by Martian Water Ice Clouds
Authors: Cooper B. , de la Torre Juárez M. , Mischna M. , Lemmon M. , Martínez G. , Kass D. , Vasavada A. R. , Campbell C. , Moores J. 
Published: December 2021

AbstractWe explore the potential role of clouds in moderating the nighttime temperature within Gale crater, as observed by the Rover Environmental Monitoring Station (REMS) instrument suite aboard the Curiosity rover. Just prior to aphelion, the decreasing trend in minimum daily temperature within Gale slows down. We investigate if this is due to increased formation of twilight and nighttime clouds, re‐radiating heat and reducing atmospheric and surface cooling. While diurnal analysis of REMS temperatures shows brief atmospheric warmings of 3–5 K post‐sunset during the occurrence of these clouds, an absence of similar warmings in the ground temperature measurements make it unlikely that clouds are the primary source. Seasonally, however, clouds that persist overnight could cause a warming of daily minimum surface temperatures in the Ls∼20°–50° season. This period can serve as a baseline and allow the potential effects of clouds to be more clearly discerned in the REMS temperature measurements. For this season, and in the peak of the aphelion cloud belt season, our modeled atmospheric energy budget shows a nocturnal decay signature of downward IR reflected and re‐emitted flux consistent with the presence and impact of clouds. The expected approximate exponential decay of this flux post‐sunset is damped more heavily in cloudier seasons than less cloudy or dusty seasons, suggesting formation and thickening of ice clouds as atmospheric nighttime temperatures cool.
Quantitative Risk Assessment of Population Affected by Tropical Cyclones Through Joint Consideration of Extreme Precipitation and Strong Wind—A Case Study of Hainan Province
Authors: Meng Chenna , Xu Wei , Qiao Yu , Liao Xinli , Qin Lianjie 
Published: December 2021

AbstractTropical cyclone disasters frequently occur in the southeastern coastal areas of China, and the concentration of population and economy has further increased the risk of tropical cyclone disasters in this region. The effects of tropical cyclones on populations involve a complex interaction between hazard intensity, environmental factors, exposure level, and fortification capacity. Therefore, quantifying the relationship among these factors is imperative for early warnings, risk assessments, and mitigation strategies for tropical cyclone disasters. In this study, a comprehensive tropical cyclone quantitative risk assessment approach considering the joint relation between strong wind and extreme precipitation and environmental, exposure and fortification factors was developed using the generalized additive model (GAM) and copula function for the case of Hainan province, China. The 10 min maximum wind speed (MW), maximum daily rainfall (MR), elevation (ELE), total population (POP), and time (t) were ultimately selected by the GAM as explanatory variables for the affected population rate (APR). A 50% increase in theMWproduced a 48.779%–82.657% increase in theAPR, a 50% increase in theMRproduced a 19.534%–25.631% increase in theAPR, and a 50% increase in the POP produced a 0.796%–15.193% decrease in theAPR. TheAPRobviously increased with the return period increase in Hainan province. TheAPRof cites (counties) ranged from 0.443 to 0.632 for a once‐in‐10‐year return period and ranged from 0.518 to 0.829 for a once‐in‐50‐year return period.
Uncertainty in Health Impact Assessments of Smoke From a Wildfire Event
Authors: Johnson Megan M. , Garcia‐Menendez Fernando 
Published: December 2021

AbstractWildfires cause elevated air pollution that can be detrimental to human health. However, health impact assessments associated with emissions from wildfire events are subject to uncertainty arising from different sources. Here, we quantify and compare major uncertainties in mortality and morbidity outcomes of exposure to fine particulate matter (PM2.5) pollution estimated for a series of wildfires in the Southeastern U.S. We present an approach to compare uncertainty in estimated health impacts specifically due to two driving factors, wildfire‐related smoke PM2.5 fields and variability in concentration‐response parameters from epidemiologic studies of ambient and smoke PM2.5. This analysis, focused on the 2016 Southeastern wildfires, suggests that emissions from these fires had public health consequences in North Carolina. Using several methods based on publicly available monitor data and atmospheric models to represent wildfire‐attributable PM2.5, we estimate impacts on several health outcomes and quantify associated uncertainty. Multiple concentration‐response parameters derived from studies of ambient and wildfire‐specific PM2.5 are used to assess health‐related uncertainty. Results show large variability and uncertainty in wildfire impact estimates, with comparable uncertainties due to the smoke pollution fields and health response parameters for some outcomes, but substantially larger health‐related uncertainty for several outcomes. Consideration of these uncertainties can support efforts to improve estimates of wildfire impacts and inform fire‐related decision‐making.
Observational Properties of 15MLT‐PCA in the Southern Hemisphere and the Switching Effects of IMF By on 15MLT‐PCA Occurrence
Authors: Feng Hui‐Ting , Han De‐Sheng , Qiu Hui‐Xuan , Shi Run , Yang Hui‐Gen , Zhang Y.‐L. 
Published: December 2021

AbstractBased on Special Sensor Ultraviolet Spectrographic Imager on Defense Meteorological Satellite Program spacecraft (DMSP/SSUSI) observations in the Northern Hemisphere, a particular polar cap arc observed at ∼1,500 magnetic local time (MLT) sector was identified and was named 15MLT‐PCA. A 15MLT‐PCA model proposed for the Northern Hemisphere suggests that the 15MLT‐PCA is caused by a lobe reconnection related to the tilt angle and lobe field line twisting due to a long‐lasting positive interplanetary magnetic field (IMF) By condition. However, it is unclear if the 15MLT‐PCA can be observed in the Southern Hemisphere and how the IMF By influences its occurrence and location. Using the long‐term DMSP/SSUSI observations in the Southern Hemisphere, we confirmed that the 15MLT‐PCA could be observed in the Southern Hemisphere in summer under long‐lasting negative IMF By and predominantly positive IMF Bx. The ion energy dispersion and some other statistical properties are also investigated. Further, we showed that the IMF By has a switching effect on the occurrence of the 15MLT‐PCA using multiple cases. We found that the 15MLT‐PCA disappear as soon as the IMF By changed to an unfavorable condition, but it seems that the favorable conditions need to be maintained for ∼1 hr for the occurrence of 15MLT‐PCA. We also found that the IMF By magnitude is well correlated to the MLT location of the 15MLT‐PCA. We suggest that all the results support the 15MLT‐PCA model and imply that a magnetotail lobe reconnection should be common in the summer hemisphere because the well‐known Parker Spiral favors meeting the model's IMF requirements.
Impact of the Solar Wind Dynamic Pressure on the Field‐Aligned Currents in the Magnetotail: Cluster Observation
Authors: Cheng Z. W. , Shi J. K. , Torkar K. , Lu G. P. , Dunlop M. W. , Carr C. M. , Rème H. , Dandouras I. , Fazakerley A. 
Published: December 2021

AbstractWe statistically investigate the influence of the solar wind dynamic pressure (SW Pdyn) on the field‐aligned currents (FACs) in the magnetotail with 1,492 FAC cases from July to October in 2001 and 2004, which covers 74 Cluster crossings of the plasma sheet boundary layer (PSBL) in both storm time and non‐storm time. The FAC density in the magnetotail is derived from the magnetic field data with the four‐point measurement of Cluster, and the SW Pdyn is taken from ACE data. The results indicate the FAC density becomes stronger with increasing SW Pdyn. The statistics show that the FAC occurrence increased monotonically with SW Pdyn in the three levels (Weak: SW Pdyn < 2 nPa; Medium: 2 nPa ≤ SW Pdyn ≤ 5 nPa; Strong: SW Pdyn > 5 nPa). The FAC density increased with increasing SW Pdyn, while its footprint (invariant latitude, ILAT) in the polar region decreased with increasing SW Pdyn. The response of the FAC to SW Pdyn in the magnetotail had a north‐south hemispheric asymmetry. The FAC density had a better correlation with SW Pdyn in the Northern hemisphere, while the footprint had a better correlation with SW Pdyn in the Southern hemisphere. Possible underlying mechanisms for our results are analyzed and discussed. However, it requires more observations and simulation studies to find out the mechanism of north‐south asymmetry.
An Evaluation of Erosional‐Geodynamic Thresholds for Rapid Orogenic Denudation
Authors: Spencer Brandon M. , Thigpen J. Ryan , Gallen Sean F. , Dortch Jason M. , Hodges Kip V. , Law Richard D. , Mako Calvin A. 
Published: December 2021

AbstractTectonic and surface processes (e.g., erosion) act in concert to denude and flatten elevated topography during and following collisional uplift. In orogens that collapsed rapidly (<10 Myr), the effectiveness and relative contribution of erosion remains poorly understood. Here, we use 1‐D and 2‐D finite‐difference models to test plausible magnitudes of erosion during denudation of the Scandian orogenic wedge, in northern Scotland, which previous work suggests may have been very rapid. Thermochronologic, stratigraphic, and thermobarometric data constrain initial post‐collision uplift rates as well as the cumulative post‐collisional exhumation magnitude. Using these constraints, we consider an idealized orogen subjected to fluvial incision, hillslope diffusion, and subsequent isostatic compensation for a duration of 10 Myr. Using a range of geomorphic parameters, we evaluate whether erosion alone is sufficient to achieve the observed cumulative exhumation. Additionally, we apply a thermal model during the same period to determine the viability of the previous erosion estimates in the presence of a transient geothermal gradient. For most cases with initial uplift rates of 0.5–2.5 mm yr−1, total erosion is insufficient to account for the observed Scandian denudation history, regardless of whether a fixed or transient geothermal gradient is assumed. Average elevations near the crest of the modeled orogen after 10 Myr are >1.4 km, which is inconsistent with the post‐Scandian landscape relief interpreted from the reconstructed depositional surface of the Emsian (407–403 Ma) Old Red Sandstone, Collectively these findings imply a combination of rapid erosion and tectonic denudation likely accomplished the rapid collapse of the Scandian wedge.
Cascading Weather Events Amplify the Coastal Thermal Conditions Prior to the Shelf Transit of Hurricane Sally (2020)
Authors: Dzwonkowski B. , Fournier S. , Lockridge G. , Coogan J. , Liu Z. , Park K. 
Published: December 2021

AbstractChanges in tropical cyclone intensity prior to landfall represent a significant risk to human life and coastal infrastructure. Such changes can be influenced by shelf water temperatures through their role in mediating heat exchange between the ocean and atmosphere. However, the evolution of shelf sea surface temperature during a storm is dependent on the initial thermal conditions of the water column, information that is often unavailable. Here, observational data from multiple monitoring stations and satellite sensors were used to identify the sequence of events that led to the development of storm‐favorable thermal conditions in the Mississippi Bight prior to the transit of Hurricane Sally (2020), a storm that rapidly intensified over the shelf. The annual peak in depth‐average temperature of >29°C that occurred prior to the arrival of Hurricane Sally was the result of two distinct warming periods caused by a cascade of weather events. The event sequence transitioned the system from below average to above average thermal conditions over a 25‐day period. The transition was initiated with the passage of Hurricane Marco (2020), which mixed the upper water column, transferring heat downward and minimizing the cold bottom water reserved over the shelf. The subsequent reheating of the upper ocean by surface heat flux from the atmosphere, followed by downwelling winds, effectively elevated shelf‐wide thermal conditions for the subsequent storm, Hurricane Sally. The coupling of climatological downwelling winds and warm sea surface temperature suggest regions with such characteristics are at an elevated risk for storm intensification over the shelf.
Radiative Effects of Particular Matters on Ozone Pollution in Six North China Cities
Authors: Li Shaomin , Liu Rui , Wang Shuai , Chen Song Xi 
Published: December 2021

AbstractWhile the particulate matters (PM) concentration has been reduced across North China in recent years, the surface ozone level has been increasing in many cities in the region. There is a widely held speculation that the elevated ozone level was caused by increased solar radiation due to the reduced PM level in recent years. This study quantifies the radiation effect of PM on O3 by analyzing data from years 2013 to 2018 in six major cities in North China. We first evaluate PM's effect on the radiation by controlling meteorological factors to radiation, which include the cloud cover, the humidity and time of the day. The PM's effect on the radiation was statistically significant in all six cities, but was the least significant among all significant factors. The PM's radiative effect on the ozone was also statistically significant, but the average ozone increase due to the decreased PM level was rather small, ranging from 0 to 0.12 μg/m3 (0–0.056 ppb) in the spring and 0–0.36 μg/m3 (0–0.168 ppb) in the summer per 10 μg/m3 PM reduction. Therefore, the wide spread spring‐summer increase of the ozone level in North China should not be attributed to the radiation increase caused by the reduced PM.
Modeling the Joint Effects of Vegetation Characteristics and Soil Properties on Ecosystem Dynamics in a Panama Tropical Forest
Authors: Cheng Yanyan , Leung L. Ruby , Huang Maoyi , Koven Charles , Detto Matteo , Knox Ryan , Bisht Gautam , Bretfeld Mario , Fisher Rosie A. 
Published: December 2021

AbstractIn tropical forests, both vegetation characteristics and soil properties are important not only for controlling energy, water, and gas exchanges directly but also determining the competition among species, successional dynamics, forest structure and composition. However, the joint effects of the two factors have received limited attention in Earth system model development. Here we use a vegetation demographic model, the Functionally Assembled Terrestrial Ecosystem Simulator (FATES) implemented in the Energy Exascale Earth System Model (E3SM) Land Model (ELM), ELM‐FATES, to explore how plant traits and soil properties affect tropical forest growth and composition concurrently. A large ensemble of simulations with perturbed vegetation and soil hydrological parameters is conducted at the Barro Colorado Island, Panama. The simulations are compared against observed carbon, energy, and water fluxes. We find that soil hydrological parameters, particularly the scaling exponent of the soil retention curve (Bsw), play crucial roles in controlling forest diversity, with higher Bsw values (>7) favoring late successional species in competition, and lower Bsw values (1 ∼ 7) promoting the coexistence of early and late successional plants. Considering the additional impact of soil properties resolves a systematic bias of FATES in simulating sensible/latent heat partitioning with repercussion on water budget and plant coexistence. A greater fraction of deeper tree roots can help maintain the dry‐season soil moisture and plant gas exchange. As soil properties are as important as vegetation parameters in predicting tropical forest dynamics, more efforts are needed to improve parameterizations of soil functions and belowground processes and their interactions with aboveground vegetation dynamics.
Vehicle localisation and deep model for automatic calibration of monocular camera in expressway scenes
Authors: Zhang Wentao , Song Huansheng , Liu Lichen , Li Congliang , Mu Bochen , Gao Qian 
Published: December 2021

AbstractThe authors present a fully automatic method for camera calibration in expressway scenes and vehicle localisation on curved roads. Our approach does not depend on specific targets or a priori information and adapts to a wide range of variations in road appearances and camera views. We make three main contributions to automatic camera calibration. Firstly, we propose a Deep Calibration Network to estimate the vanishing point and camera extrinsic parameters from an RGB image in an end‐to‐end manner. The vanishing point and camera rotation angles are then used to calculate the camera focal length according to the perspective projection geometry, thus constructing the complete camera matrix. Secondly, we introduce a Multi‐View Camera Calibration Dataset, which contains a total of 32,712 images for 17 expressway scenes. The dataset covers a wide range of camera views and expressway types, which is challenging. Thirdly, we present a novel Curved Reference Line System adapted to the expressway shape to locate vehicles on curved roads accurately. This coordinate system can effectively transform the vehicle coordinates from a Cartesian system to the vehicle's mileage and horizontal distance relative to the lane line. A comprehensive evaluation on Multi‐View Camera Calibration Dataset shows that our technique can automatically calibrate a single camera at about 40fps on an NVIDIA GTX1060 processor and accurately locate vehicles on curved roads, making it suitable for deployment in expressway surveillance systems.
Smokeless Tobacco and Its Ill-Effects: Recent Literature Update
Authors: Singhavi Hitesh R. , Singh Arjun , Chaturvedi Pankaj 
Published: December 2021

AbstractAccording to the Global Adult Tobacco Survey part 2 (GATS-2), approximately 200 million adult Indians consume smokeless tobacco (SLT). Circumvention of SLT product ban has been observed. We conducted a review of the literature to summarize the ill effects of tobacco for the articles published from 2019 to 2020. A systematic search of the databases PubMed (2019 onward) and Web of Science (2019 onward), through February 2021 was done. Search yielded 1,061 articles and after excluding articles based on the inclusion criteria, 37 articles were taken in to consideration. The review shows that differential SLT product has specific odds of oral carcinogenesis. Review also indicates the emerging data of cardiovascular risk due to higher use of SLT products along with its known cause of oral cancer. It also cautions about the adverse consequences of pregnancy associated with SLT use.