bluuebunny/crossref_metadata_2025_split huggingface dataset:
Contents and Quality of Malaria Information in Spanish Travel Blogs
Authors: Linares-Rufo Manuel , Santos-Larrégola Laura , Górgolas-Hernández-de-Mora Miguel , Ramos-Rincon José-Manuel 
Published: May 2021

Abstract
Background. About 10,000 imported cases of malaria are reported each year in Europe; 80 per 100,000 trips to endemic areas. Non-use of chemoprophylaxis in travelers remains the main reason for this. Travel blogs have proliferated throughout the network being a very common place to consult, sometimes the only one used, before preparing a trip to an endemic area. The aim of this study was to analyze the information offered on malaria in the main travel blogs in Spanish. Methods: we selected and analyzed 100 travel blogs that were considered highly relevant by its metrics: by 1) Social networks (RRSS) measuring the total followers of Facebook, Twitter, Instagram and YouTube), 2) number of monthly visits using the SEMrush tool; 3) Domain authority and 4) number of backlinks or incoming links considering the age and the percentage according to category using the SEMrush tool.Results: To this end, the 100 Spanish-language travel blogs with the greatest impact were selected and analyzed. The results showed a very low quality of information provided by these publications. The contents offered were generally insufficient, incomplete or, more seriously, inadequate. In many cases, this could encourage the user not to take the appropriate preventive measures.Conclusions: This study showed a low quality of travel blogs in Spanish regarding malaria. The so-called “travel influencers” must communicate reliable, verified and quality information on malaria on their channels in a way that could contribute to a reduction in the burden of the disease in our environment
Influence of cord blood serum and actovegin on the reproductive function of cows in the comparative aspect
Authors: Bondarenko Irina , Lazorenko Andrey , Shkromada Oksana 
Published: May 2021

The results of the study of the effect of cord blood serum and “Actovegin” (Takeda, Ukraine) on the reproductive function of cows in a comparative aspect are presented.
The aim of the study was to analyze the effect of cord blood serum and Actovegin on the reproductive function of uterine cows in a comparative aspect.
Materials and methods. The research was conducted during 2017-2020 in four dairy farms of Sumy region with tethered and untied maintenance. In 128 cows of different breeds and productivity, the timing of sexual cycling was studied and analyzed in comparison with the spontaneous sexual cycle with the use of cord blood serum and the drug "Actovegin" (Takeda, Ukraine).
Results. It was found that in cows with tethered content, the number of days from the introduction of 15 ml of cord blood serum to the manifestation of the excitation stage was significantly less compared to the spontaneous manifestation of sexual desire (5.63±0.36 and 14.38±2.1, respectively).
When cows with loose content were administered 10 ml of cord blood serum in combination with the drug "Actovegin", the number of days before the manifestation of sexual cycling was lower by 18.3 % compared with the rate of spontaneous sexual desire (4.31±0.38 and 18.25±1.89, respectively).
Conclusions. As a result of using 10 ml of cord blood serum in combination with the drug "Actovegin", in cows of different productivity and under different housing conditions, the number of days before the onset of the stage of arousal is significantly reduced compared to the spontaneous manifestation of sexual desire.
Cows of different productivity and with different housing conditions come to the sexual desire significantly faster with the use of 15 ml of cord blood serum compared to the spontaneous manifestation of sexual desire.
Histotopographic and morphometric characteristics of the oral mucosa postoperative wounds healing, depending on the method of connecting the wound edges
Authors: Pavlenko Oleksiy , Boiko Mikola , Savitskaya Irina , Geylenko Olga 
Published: May 2021

Implementation of high-quality tissue connections and hemostasis in oral surgery is an urgent problem of modern dentistry, due to the lack of available universal methods that would help to facilitate the work of oral surgeon and reduce wound healing time.
The aim of the research. To investigate and study the regeneration processes in the oral mucosa after high-frequency electric welding, suturing and medical adhesive composition.
Materials and methods. This study compared the effect of three connection methods and hemostasis: high-frequency electric welding (EKVZ-300M1 “PATONMED®”, Ukraine), suture method (nylon 5/0 “PIRUS®”, China), adhesive composition (“Histoacryl®”, Germany). 72 rabbits were involved in experiment. An incision of the soft tissues of the vestibulum of the oral cavity of the maxilla with a length of 1.3–1.5 cm was made. On days 3, 7, 14 and 21, tissue samples were excised, histological and morphometric examination of the material were performed.
Results. By statistical data processing of 72 rabbits was found that the reliability of differences in the levels of morphometric parameters between groups on each day is very high, as evidenced by the calculated values ​​of the U-test. In 38 cases (90.5 %), the significance level was p≤0.01, in the other 4 cases – p≤0.05. The lowest value level of neutrophilic granulocytes was in rabbits of group No.1 (electric welding). In rabbits of group No. 1 GFBC were not observed in the 8 fields of view throughout the experiment.
Conclusions. High-frequency electric welding of soft tissues can be used for effective intraoperative hemostasis and connection of the intraoral wound edges.
Due to the absence of a foreign body (adhesive masses, filaments) and moderate alterative effect, histological examination indicates accelerated activation of regenerative processes in the early stages of observation in rabbits of group No. 1.
Combined prevention of fetal growth restriction based on determination of diagnostic markers
Authors: Golyanovskyi Vladimir 
Published: May 2021

Due to the morbidity of mothers and newborns caused by fetal growth restriction (FGR) and preeclampsia, preventive measures should be taken, especially in women at high risk of developing these conditions. Many studies have been conducted on the prevention of FGR and preeclampsia in high-risk women, especially anticoagulants, aspirin, paravastatin, nitric oxide, microelements (L-arginine, folic acid, vitamins E and C, phytonutrients, vitamin D) and calcium.
The aim is to improve perinatal consequences by preventing FGR in high-risk women.
Materials and methods: A prospective study of 137 pregnant women in the period of 110–136 weeks was conducted at the Perinatal Center in Kyiv. Pregnant women were divided into 3 groups. The main group included 47 women at high risk of FGR who received therapy (low doses of aspirin, low molecular weight heparin (LMWH) and vitamin and micro elements drugs). The comparison group included 45 women who had a high risk of FGR but did not receive treatment. The control group consisted of 45 women who were not at risk of FGR. The frequency of FGR and placental dysfunction were analyzed as well as a fetal distress was analyzed ante- and intranatally.
Results: Therapy with low doses of aspirin, LMWH and a complex preparation of vitamins and micro elements improves the course of pregnancy and gestational complications. In the main group FGR was detected in 8.5 %, in the comparison group – in 17.8 %, in the control group – 4.4 %. Placental dysfunction was detected in 13.3 % in the control group, and only 6.4 % in the main group that was close to the control group – 2.2 %. Similar tendencies were found for fetal distress ante- and intranatally.
Conclusions: The proposed prophylactic measures can improve maternal outcomes by reducing the level of gestational complications in pregnant women with biochemical signs of risk of FGR development. In addition, these preventive measures can reduce the frequency of children births with growth restriction, which significantly reduces early neonatal and perinatal morbidity and mortality.
Variations in pretransfusion practices
Authors: Padget B.J. , Hannon J.L. 
Published: May 2021

Abstract
A variety of pretransfusion tests have been developed to improve the safety and effectiveness of transfusion. Recently, a number of traditional tests have been shown to offer limited clinical benefit and have been eliminated in many facilities. A survey of pretransfusion test practices was distributed to 116 hospital transfusion services. Routine test practices and facility size were analyzed. Ninety-one responses were received. Many smaller laboratories include tests such as anti-A,B, an autocontrol, and DAT, and immediate spin and 37°C microscopic readings. Nine percent never perform an Rh control with anti-D typing on patient samples. Various antibody screening and crossmatch methods are utilized. Individual laboratory test practices should be periodically assessed to ensure that they comply with standards, represent the recognized best practice, and are cost-effective. The survey responses indicate that many laboratories perform tests that are not necessary or cost-effective. These facilities should review their processes to determine which tests contribute to transfusion safety. Smaller facilities may be reluctant to change or lack the expertise necessary for this decision making and often continue to perform tests that have been eliminated in larger facilities. Consultation with larger hospital transfusion services may provide guidance for this change.
Immunohematology 2003;19:1–6.
Comparative testing for weak expression of D antigen: manual tube testing vs. a semiautomated IgG gel system
Authors: Martinez B. , Crews E. , Dowd A. , Mcmahan M. 
Published: May 2021

Abstract
Donor RBCs nonreactive in initial tests for D must be tested further for evidence of weak expression of D antigen. Performing this test in test tubes is labor intensive and prone to inconsistencies in readings (relative strength of agglutination) and interpretation (positive versus negative). These inconsistencies can lead to repeat testing, additional documentation, and delay in releasing units. We evaluated use of the Tecan MEGAFlex-ID™ pipettor to perform this test in anti-IgG gel cards. Results with this semi-automated gel test were compared with results obtained with 37 D– and 99 weak D samples, as determined by previous testing with a manual IAT tube test. Hands-on time was determined for both methods and both methods were evaluated for inconsistency, or nonagreement, between the interpretation of the current weak D test and the results on record for any prior donations. There were no discordant results obtained, with the majority of weak D samples giving stronger reactions with the gel test. The semiautomated gel test required less hands-on time, with an average savings of more than 70 seconds per test. There were no inconsistencies with the gel method, whereas manual tube testing was found to have an inconsistency rate of 0.035 percent of total samples tested. Semiautomated IgG gel is now used for all weak D testing, with a labor savings of more than 10 hours per week. Thus far, more than 70,000 donors have been tested, with no inconsistencies reported.
Immunohematology 2003;19:7–9.
Neonatal alloimmune thrombocytopenia due to anti-HPA-2b (anti-Koa)
Authors: Goldman M. , Trudel E. , Richard L. , Khalife S. , Spurll G.M. 
Published: May 2021

Abstract
Most severe cases of neonatal alloimmune thrombocytopenia (NAIT) are due to anti-HPA-la (anti-PlA1) antibodies. We report a case of NAIT due to anti-HPA-2b that resulted in in utero intracranial hemorrhage. A 33-year-old G2P1A0 Caucasian woman had a routine ultrasound at 34 weeks. The fetus appeared to have a left hemispheric hematoma. ΓVIG, lg/kg, was started immediately and administered weekly until delivery. One day after receiving the first dose of ΓVIG, fetal platelet count was 18 × 109/L, and Hb was 116 g/L. Eleven mL of matched platelets compatible by monoclonal antibody immobilization of platelet antigens (MAIPA) assay were transfused in utero, raising the platelet count to 62 × 109/L. Repeat transfusions were done later that week and 1 week later, with pretransfusion counts of 19 × 109/L and 16 × 109/L, respectively. Delivery by C section was done at 35.5 weeks, after the third platelet transfusion. Platelet count at birth was 77 × 109/L. Drainage of the hematoma was performed after transfusion. Testing with a solid phase ELISA revealed reactivity against GPlb/IX. MAIPA testing after platelet treatment with the protease inhibitor leupeptin demonstrated the presence of anti-HPA-2b. On PCR-SSP the mother was HPA-2a homozygous, the father was HPA-2a/2b. Antibodies against the HPA-2b antigen located on the GPlb/IX complex have been reported in rare cases of NAIT Testing is complicated by proteolytic degradation of the antigen-bearing fragment. Compatible platelets are easily found since approximately 85 percent of donors are HPA-2a/2a.
Immunohematotogy 2003;19:43-46.
Quantitation of red cell–bound IgG, IgA, and IgM in patients with autoimmune hemolytic anemia and blood donors by enzyme-linked immunosorbent assay
Authors: Bencomo A. A. , Diaz M. , Alfonso Y. , ValdÉs O. , Alfonso M. E. 
Published: May 2021

Abstract
This paper describes an enzyme immunoassay for the quantitative determination of IgG, IgA, and IgM immunoglobulins on RBCs. Ether eluates made from RBCs were followed by an enzyme-linked immunosorbent assay of immunoglobulin concentration. Calibration curves were derived from immunoglobulin standards and the number of molecules of each isotype per RBC was calculated. The assay was carried out in 200 healthy blood donors and 62 patients with warm autoimmune hemolytic anemia (AIHA), two of them with a negative DAT. For healthy blood donors, mean values were 58 IgG, 16 IgA, and 3 IgM molecules per RBC. For patients with a positive DAT, the mean values were 3435 IgG, 157 IgA, and 69 IgM molecules per RBC. An increased level of IgA was found in 12 patients without IgA autoantibodies demonstrable in RBC eluates. Increased IgG levels were also observed in patients with a negative DAT and, in one case, an increased level of IgA was also found. The enzyme-linked immunosorbent assay using ether eluates is a sensitive method for quantitating RBC autoantibodies in patients with AIHA as well as immunoglobulins bound to RBCs in healthy individuals.
Immunohematology 2003;19:47–53.
Studies on the Dombrock blood group system in non-human primates
Authors: Mogos C. , Schawalder A. , Halverson G.R. , Reid M.E. 
Published: May 2021

Abstract
The Dombrock blood group system consists of five distinct antigens: two antithetical antigens, Doa and Dob, and three high-frequency antigens: Gya, Hy, and Joa. Although the prevalence of Doa and Dob in different populations makes them useful as genetic markers, the scarcity of reliable antibodies to these antigens has prevented this potential from being realized. The gene (DO; ART4) encoding the Dombrock glycoprotein has been cloned and sequenced, and the molecular bases of the various Dombrock phenotypes have been determined. The purpose of this study was to perform DNA-based assays on the DO homolog in non-human primates to determine the degree of conservation in the DO gene. Murine MoAbs to Dombrock protein were developed by standard hybridoma technologies and used to test RBCs from non-human primates by hemagglutination. PCR-RFLP analysis for the six singlenucleotide polymorphisms (SNPs) that have been defined in human alleles were performed on DNA extracted from fresh or frozen blood samples from numerous non-human primates. Hemagglutination tests with six MoAbs to the Dombrock glycoprotein revealed distinct epitopes on RBCs from the non-human primates. The gorillas and orangutans had the same PCR-RFLP digestion pattern for the six SNPs studied as chimpanzees. Old world monkeys (macaques) were identical at nucleotides (nt) 323, 350, 624, and 793 with the chimpanzees, and at nt 898 the digestion pattern was the same as for the HY1 allele in humans. For the new world monkeys (tamarins and squirrel monkeys) the digestion pattern was conserved for nt 793 but different for nt 624; the other SNPs could not be determined because there was no amplification. The presence of epitopes recognized by the MoAbs and PCR-RFLP results among the non-human primates shows considerable conservation of the DO gene. The difficulties we encountered with the amplification of DNA from the non-human primates lower in the phylogenetic tree are probably due to divergence in sequence.
Practical aspects of investigating drug-induced immune hemolytic anemia due to cefotetan or ceftriaxone—a case study approach
Authors: Arndt P.A. 
Published: May 2021

Abstract
In the 1970s, the most common causes of drug-induced immune hemolytic anemia were methyldopa and penicillin. Since 1990, the most common causes of drug-induced immune hemolytic anemia have been the second- and third-generation cephalosporins, cefotetan and ceftriaxone. Three case histories illustrate the common findings in the serologic investigation of immune hemolytic anemias due to these two drugs.
Immunohematology 2002;18:27–32.
Neonatal alloimmune thrombocytopenia due to HPA-3a antibodies: a case report
Authors: Davoren A. , Smith G. , Lucas G. , Rodgers S. , O’donoghue P. , Crowley J. , Barnes C.A. , Mckiernan J. 
Published: May 2021

Abstract
A healthy infant was born at term by elective cesarean section to a 32-year-old para 4, gravida 4, mother. Within 24 hours, the infant was noted to have fairly extensive bruising on the back and shoulders. A full blood count evaluation was remarkable for severe thrombocytopenia (platelet count of 29 × 109/L). Other hematologic parameters were normal. Human leukocyte antigen (HLA) class-1 antibodies but not platelet-specific antibodies were detectable in the maternal serum using a commerical antigencapture ELISA (GTI-PakPlus kit®). Anti-HPA-3a antibodies, while weakly reactive in the monoclonal antibody immobilization of platelet antigens (MAIPA) assay in the immediate postpartum serum, were readily detectable using this assay in a sample taken 4 weeks later. Genotyping for human platelet antigens (HPA) 1–5 by the polymerase chain reaction technique with sequence-specific primers (PCR-SSP) revealed the infant’s platelet genotype to be HPA-1a/1a, 3a/3b, while that of the mother was HPA-1a/1a, 3b/ 3b, consistent with a diagnosis of anti-HPA-3a neonatal alloimmune thrombocytopenia (NAIT). This case illustrates the increased sensitivity of the MAIPA technique for the detection of plateletspecific antibodies. We believe this to be the first serologically confirmed case of NAIT due to anti-HPA-3a to be reported in the republic of Ireland.
Immunohematology 2002;18:33–36.
MIMA-9, a valuable antibody for screening for rare donors
Authors: Tossas E. , Øyen R. , Halverson G.R. , Malyska H. , Reid M.E. 
Published: May 2021

Abstract
Since monoclonal antibodies (Mabs) are potentially available in an unlimited volume, they can be used to screen numerous donor blood samples to identify antigen-negative donors. We have used a Mab (MIMA-9) with characteristics that allow for the simultaneous screening of RBCs of any ABO group for high-incidence antigennegativity in the Kell and Gerbich blood group systems. MIMA-9, a murine IgG2a antibody, previously shown to facilitate the identification of K+k–, Kp(a+b–), K0, McLeod, or Ge:–3 red blood cells (RBCs), was used in MTS gel cards containing anti-mouse IgG as the second antibody to test 1134 K– donors. Among the 1134 donors tested, we found one Kp(a+b–) and one Ge:–2,–3,4 donor. If random donor samples had been used instead of preselecting for K–, we would have expected to identify two K+k– donors. One reagent (MIMA-9) can be used to simultaneously screen for K+k–, Kp(a+b–), K0, McLeod, and Ge:–3 RBCs and thereby conserve rare antisera. Inclusion of anti-mouse IgG in gel cards allowed for rapid screening. MIMA-9 is also a useful reagent to type RBCs with a positive direct antiglobulin test. This antibody is available to donor screening laboratories at no cost for this specific use.
Immunohematology 2002:18:43–45.
Enzyme and DTT treatment of adherent RBCs for antibody identification by a solid phase immunoassay system
Authors: Tamai T. , Mazda T. 
Published: May 2021

Abstract
Treatment of RBCs with protease enzymes or dithiothreitol (DTT) causes denaturation of several RBC antigens and is regularly used in antibody identification. In this study, we have standardized enzyme and DTT treatment of adherent RBCs in the magnetic-mixed passive hemagglutination assay (M-MPHA) for antibody identification. We have also tried drying these treated RBCs. The optimal enzyme and DTT treatment conditions for intact adherent RBCs were determined, in addition to the optimal condition for drying RBCs. All tested agents with the exception of chymotrypsin could be applied to RBC drying,but the optimal condition for drying was different from that for intact RBCs. Enzyme or DTT treatment of adherent RBCs and their application in M-MPHA provide a simple and convenient method for antibody identification. Furthermore, the technique of drying treated RBCs provides an even stronger antibody identification tool, since dried RBCs can be stored for a long period.
Immunohematology 2002;18:114–119.
Evaluation of a new solid-phase immunoassay for alloantibody detection using bromelin-treated and untreated red blood cells
Authors: Tamai T. , Mazda T. 
Published: May 2021

Abstract
The enzyme test is used to detect certain antibodies or facilitate antibody identification. This study compares antibody reactivity with bromelin-treated red blood cells (RBCs) and untreated RBCs using a newly developed solid-phase immunoassay. The reactivity of irregular antibodies was tested by a magnetic-mixed passive hemagglutination assay (M-MPHA). In addition, antibody reactivity was tested with dried stroma of bromelin-treated RBCs and untreated RBCs (M-MPHA-Dry). Rh antibodies were detected with enzyme-treated intact RBCs and untreated RBCs by M-MPHA. The slight increase in reactivity using M-MPHA was not seen using dried RBC stroma (M-MPHA-Dry). All donor-derived IgG alloantibodies, which were detected by either a conventional tube enzyme test or an indirect antiglobulin test, were detected by M-MPHA without using enzyme-treated RBCs. Both M-MPHA and M-MPHA-Dry can be used for antibody detection without using enzyme-treated RBCs and are also useful for antibody identification.
Immunobematology 2OOI;I7:I7—21.
Tube and column agglutination technology for autocontrol testing
Authors: Courtney J. E. , Vincent J.L. , Indrikovs A.J. 
Published: May 2021

Abstract
The incidence of positive autocontrol test results with column agglutination technology is a concern. This study investigates the incidence and significance of positive autocontrols in the ID Micro Typing System™ (gel) and the Gamma ReACT™ (ReACT). The study encompassed a total of 1021 randomly selected samples from patients and 95 samples from donors collected during 1 month. The autocontrol testing was carried out according to the manufacturer’s instructions for the column agglutination tests. The tube method was carried out using low-ionic-strength solution (USS). The direct antiglobulin test (DAT) was performed using the tube method, and further investigated with elution studies if warranted. Seventy-nine patient’s samples (7.74%) had a positive autocontrol: the gel test, 72 (91.13%); ReACT, 21 (26.58%); and the tube method, 27 (34.18%). Of the 79 positive autocontrols, 44 samples had a negative DAT. Of the samples with positive DAT results, only one possessed a clinically significant antibody, anti-D. Moreover, the same sample also tested positive in all three methods. Column agglutination techniques have increased sensitivity for a positive autocontrol beyond the conventional tube method. However, ReACT and gel tests differ significantly in their frequency of positives. Investigation of the significance of a positive autocontrol in column agglutination technology when the conventional tube method is also positive is suggested.
Immunobematology 2OOI;I7:5O—52.