bluuebunny/crossref_metadata_2025_split huggingface dataset:
A220 AN UNEXPECTED CAUSE OF ACUTE JAUNDICE IN A POST-TRANSPLANT PATIENT
Authors: Wong K W , Silverman J 
Published: March 2021

Abstract

Background
Complications post liver transplantation are common. These may include thromboses, primary graft nonfunction, biliary complications, chylothorax, infection, and rejection. Hepaticojejunostomy with Roux-en-Y limb is a common technique for pediatric liver transplantation. Complications of hepaticojejunostomy include small bowel obstruction due to incarceration of the Roux-en-Y limb within an internal hernia. Acquired diaphragmatic hernia has previously been reported in liver transplant patients. Here we present a unique case of acute biliary obstruction secondary to incarceration of a Roux limb within an acquired diaphragmatic hernia.

Aims
To describe a unique case of incarcerated Roux-en-Y limb in a diaphragmatic hernia leading to acute jaundice in a pediatric liver transplant patient.

Methods
Retrospective chart review and case review with the surgical and medical teams.

Results
A 14 month old girl with unresectable hepatoblastoma received a living donor, left lateral segment liver transplant using standard venous reconstruction, microvascular plastics arterial reconstruction, and the recipient common hepatic duct was anastomosed to the donor left hepatic duct. Serial ultrasounds showed persistent intrahepatic duct dilatation with cholestasis. Given the concern of biliary obstruction, 10 days post-transplant, she underwent repeat laparotomy and was found to have a dilated common bile duct with a proximal obstruction. A Roux-en-Y hepaticojejunostomy was performed to establish adequate biliary drainage.
Three months post-transplant she developed lung metastases that required wedge resection and chemotherapy. 7 months post-transplant, while admitted for routine chemotherapy she acutely developed jaundice, pruritus, and transaminitis. Liver ultrasound showed an apparent fluid collection at the cut surface of the liver and biliary dilatation. Extrinsic compression of the biliary tree was suspected, and so a percutaneous biliary drain was placed to drain the collection and decompress the biliary system.
Despite improvement in the transaminitis and cholestasis, several days later, she developed severe abdominal pain and associated grunting respirations. An MRI with MRCP to reassess the previous findings and rule out a new intraabdominal cause for her symptoms was completed and unexpectedly showed herniation of her Roux-en-Y limb into a diaphragmatic hernia. She had urgent diaphragmatic hernia repair with reduction of the incarcerated bowel loop, with resolution of her pain and jaundice.

Conclusions
Post liver transplant Roux-en-Y herniation is uncommon, but can occur with internal hernias and in diaphragmatic hernias. This uncommon complication should be considered for the post liver transplant patient with hepaticojejunostomy in the setting of acute jaundice, particularly if accompanied by acute onset abdominal pain and/or increased work of breathing.

Funding Agencies
None
A231 THE MICROBIOTA-NOCICEPTORS-MICROGLIA AXIS CONTROLS THE DEVELOPMENT AND MAINTENANCE OF CHRONIC VISCERAL HYPERSENSITIVITY
Authors: Abdullah N , Altier C 
Published: March 2021

Abstract

Background
Pain is the most common cause of disability in inflammatory bowel disease (IBD). Current medical interventions control the debilitating clinical symptoms by reducing gastrointestinal (GI) inflammation. Despite successful treatment of active disease, abdominal pain persists during remission, suggesting a high level of plasticity in pain-sensing circuits (hyperalgesic priming) caused by inflammation. What drives this remodelling has remained elusive. We have identified microglia as active players of hyperalgesic priming in IBD. Furthermore, it was recently shown that commensal bacteria control the maturation of microglia in the CNS, suggesting that dysbiosis could influence visceral sensitivity through regulating colonic nociceptors-microglia interaction. Here we test the hypothesis that microbiome-nociceptors-microglia interactions control visceral sensitivity and pain in IBD.

Aims
We investigated the role of the microbiota in the developmental regulation of colonic nociceptors that express the pain receptor TRPV1. We will identify the microbial factors that control neuron-microglia interactions during bacterial colonization and post-inflammatory dysbiosis.

Methods
We have developed a germ-free TRPV1-GFP reporter mouse to be used for a combination of behavioural tests and phenotypic characterization of TRPV1+ nociceptors. RNA-sequencing of FACS isolated TRPV1+ neurons of germ-free mice will be used to identify genes that are under the control of the microbiota. We will restore discrepancies observed in germ-free mice by recolonization to assess the impact of the microbiota. Furthermore, we will investigate the regulation of Ahr in TRPV1+ neurons by the microbiota and the effect of its ligands on microglial activation and post-inflammatory visceral pain.

Results
Measuring somatic pain sensation in naive germ-free and SPF mice, we showed a 15% reduction in thermal pain threshold, as measured by the Hargreaves test, and a 50% reduction in mechanical pain threshold, as measured by the Von Frey test, in germ-free mice. When looking at the dorsal root ganglia of germ-free and SPF mice, we saw a 15% increase in the percentage of neurons that were TRPV1-GFP positive in germ-free mice.

Conclusions
Our results thus far highlight the importance of the microbiota in regulating the lineage of nociceptive neurons and the threshold of mechanical and thermal pain responses. These findings suggest a major contribution of the microbiota in shaping the neuro-immune axis, with major implications for visceral sensitization in the context of dysbiosis. My project will be looking further into the phenotype of nociceptors in germ-free mice and the effect of microbial-derived Ahr agonists on the maturation and function of colonic TRPV1+ nociceptors. My work will advance our understanding of mechanisms by which commensal bacteria regulate GI pain.

Funding Agencies
CIHR
Susceptibility of Midge and Mosquito Vectors to SARS-CoV-2
Authors: Balaraman Velmurugan , Drolet Barbara S , Gaudreault Natasha N , Wilson William C , Owens Jeana , Bold Dashzeveg , Swanson Dustin A , Jasperson Dane C , Noronha Leela E , Richt Juergen A , Mitzel Dana N 
Published: March 2021

Abstract
SARS-CoV-2 is a recently emerged, highly contagious virus and the cause of the current COVID-19 pandemic. It is a zoonotic virus, although its animal origin is not clear yet. Person-to-person transmission occurs by inhalation of infected droplets and aerosols, or by direct contact with contaminated fomites. Arthropods transmit numerous viral, parasitic, and bacterial diseases; however, the potential role of arthropods in SARS-CoV-2 transmission is not fully understood. Thus far, a few studies have demonstrated that SARS-CoV-2 replication is not supported in cells from certain insect species nor in certain species of mosquitoes after intrathoracic inoculation. In this study, we expanded the work of SARS-CoV-2 susceptibility to biting insects after ingesting a SARS-CoV-2-infected bloodmeal. Species tested included Culicoides sonorensis (Wirth & Jones) (Diptera: Ceratopogonidae) biting midges, as well as Culex tarsalis (Coquillett) and Culex quinquefasciatus (Say) mosquitoes (Diptera: Culicidae), all known biological vectors for numerous RNA viruses. Arthropods were allowed to feed on SARS-CoV-2-spiked blood and at a time point postinfection analyzed for the presence of viral RNA and infectious virus. Additionally, cell lines derived from C. sonorensis (W8a), Aedes aegypti (Linnaeus) (Diptera: Culicidae) (C6/36), Cx. quinquefasciatus (HSU), and Cx. tarsalis (CxTrR2) were tested for SARS-CoV-2 susceptibility. Our results indicate that none of the biting insects, nor the insect cell lines evaluated support SARS-CoV-2 replication, suggesting that these species are unable to be biological vectors of SARS-CoV-2.
A Tale of Two Meckel’s: small bowel obstruction secondary to Meckel’s Diverticulum
Authors: Ebrahimi Nargus , Quinn Rakesh , Liang Yi , Curran Richard 
Published: March 2021

Abstract
We present two rare cases of small bowel obstruction (SBO) secondary to Meckel’s diverticulum (MD) where the mechanism of obstruction was not readily apparent. Both were cases of virgin abdomen with pre-operative CT scans demonstrating SBO without a clear underlying cause or mass. Diagnostic laparoscopy was performed, which established the underlying cause to be MD, and laparoscopic-assisted resection was undertaken to resect small bowel and perform a side-to-side stapled anastomosis. We subsequently describe the different mechanisms by which MD can cause obstruction as described in the literature.
Risk-Efficient Bayesian Data Synthesis for Privacy Protection
Authors: Hu Jingchen , Savitsky Terrance D , Williams Matthew R 
Published: March 2021

Abstract
Statistical agencies utilize models to synthesize respondent-level data for release to the public for privacy protection. In this study, we efficiently induce privacy protection into any Bayesian synthesis model by employing a pseudo-likelihood that exponentiates each likelihood contribution by an observation record-indexed weight ∈[0,1], defined to be inversely proportional to the identification risk for that record. We start with the marginal probability of identification risk for a record, which is composed as the probability that the identity of the record may be disclosed. Our application to the Consumer Expenditure Surveys (CE) of the U.S. Bureau of Labor Statistics demonstrates that the marginally risk-weighted synthesizer provides an overall improved privacy protection. However, the identification risks actually increase for some moderate-risk records after risk-weighted pseudo-posterior estimation synthesis owing to increased isolation after weighting, a phenomenon we label “whack-a-mole.” We proceed to construct a weight for each record from a collection of pairwise identification risk probabilities with other records, where each pairwise probability measures the joint probability of reidentification of the pair of records, which mitigates the whack-a-mole issue and produces a more efficient set of synthetic data with lower risk and higher utility for the CE data.
Diagnosis of Eating Disorders Among College Students: A Comparison of Military and Civilian Students
Authors: Falvey Sarah E , Hahn Samantha L , Anderson Olivia S , Lipson Sarah K , Sonneville Kendrin R 
Published: March 2021

ABSTRACTIntroductionEating disorders are often under-detected, which poses a serious threat to the health of individuals with eating disorder symptoms. There is evidence to suggest that the military represents a subpopulation that may be susceptible to high prevalence of eating disorders and vulnerable to their underdiagnosis. Underreporting of eating disorder symptoms in the military could lead to this underdiagnosis of individuals with eating disorder symptoms. The purpose of this study was to examine the association between military affiliation and eating disorder symptoms among college students and the likelihood of eating disorder diagnosis among those with eating disorder symptoms using a large, diverse college-aged sample of both military-involved and civilian students.Materials and MethodsParticipants for this study were from the 2015-2016, 2016-2017, and 2017-2018 Healthy Minds Study (HMS). Healthy Minds Study is a large, cross-sectional cohort study of both undergraduate and graduate students from universities and colleges across the United States and Canada. The Healthy Minds Study survey questions include assessment of demographic information, military status, self-reported eating disorder symptoms using the SCOFF questionnaire, and self-reported eating disorder diagnosis. Univariate analysis, chi-square analysis, and logistic regression with an unadjusted and covariate adjusted model were used to examine the association between eating disorder symptoms and military affiliation. These analyses were also used to examine the association between eating disorder diagnosis among those with eating disorder symptoms and military affiliation. All analyses were conducted using SPSS.ResultsThe prevalence of eating disorder symptoms was high among both the civilian (20.4%) and military-involved (14.4%) students. Among females, there was a significantly higher (P value = .041) prevalence of eating disorder symptoms among civilian college students (24.7%) compared to military-involved students (21.3%). Among those with eating disorder symptoms, the prevalence of diagnosis was low in both military and civilian students. Specifically, the prevalence of diagnosis was significantly lower (P value = .032) among military-involved college students (10.8%) compared to civilian college students (16.4%). Differences in sociodemographic characteristics (e.g., gender, race/ethnicity, and age) among military-involved and civilian college students appear to explain this association.ConclusionsThe underdiagnosis of eating disorders is far too common, and this represents a threat to the health of military and civilian populations alike. Underdiagnosis of eating disorders within military environments may be due to underreporting, particularly among men and racial/ethnic minority groups.
ConsHMM Atlas: conservation state annotations for major genomes and human genetic variation
Authors: Arneson Adriana , Felsheim Brooke , Chien Jennifer , Ernst Jason 
Published: March 2021

Abstract
ConsHMM is a method recently introduced to annotate genomes into conservation states, which are defined based on the combinatorial and spatial patterns of which species align to and match a reference genome in a multi-species DNA sequence alignment. Previously, ConsHMM was only applied to a single genome for one multi-species sequence alignment. Here, we apply ConsHMM to produce 22 additional genome annotations covering human and seven other organisms for a variety of multi-species alignments. Additionally, we extend ConsHMM to generate allele-specific annotations, which we use to produce conservation state annotations for every possible single-nucleotide mutation in the human genome. Finally, we provide a web interface to interactively visualize parameters and annotation enrichments for ConsHMM models. These annotations and visualizations comprise the ConsHMM Atlas, which we expect will be a valuable resource for analyzing a variety of major genomes and genetic variation.
Family Matters: Education and the (Conditional) Effect of State Indoctrination in China
Authors: Kao Jay C 
Published: March 2021

Abstract
When and how does state indoctrination work? Building upon research on motivated reasoning and family socialization, I argue that only those individuals whose parents have connections to political patronage are subject to state indoctrination because their pro-regime biases transmitted from parents induce higher receptivity prior to government messages. Focusing on political education in China, I conduct a quasi-experimental analysis exploiting the sharp variation in textbook content generated by China’s most recent curriculum reform. Results based on a national survey show that the new politics textbooks successfully affected only those individuals whose parents had worked for the government. The finding survives extensive robustness checks and falsification tests. I also consider several alternative explanations of the effects: preference falsification, selective attention, parental indoctrination, and educational quality. This paper not only highlights the role of intergenerational transmission in moderating the effectiveness of state indoctrination but also casts doubt on the actual degree to which regimes can change minds by changing educational content.
Faster than warp speed: early attention to COVD-19 by anti-vaccine groups on Facebook
Authors: Kalichman Seth C , Eaton Lisa A , Earnshaw Valerie A , Brousseau Natalie 
Published: March 2021

Abstract

Background
The unprecedented rapid development of COVID-19 vaccines has faced SARS-CoV- (COVID-19) vaccine hesitancy, which is partially fueled by the misinformation and conspiracy theories propagated by anti-vaccine groups on social media. Research is needed to better understand the early COVID-19 anti-vaccine activities on social media.

Methods
This study chronicles the social media posts concerning COVID-19 and COVID-19 vaccines by leading anti-vaccine groups (Dr Tenpenny on Vaccines, the National Vaccine Information Center [NVIC] the Vaccination Information Network [VINE]) and Vaccine Machine in the early months of the COVID-19 pandemic (February–May 2020).

Results
Analysis of 2060 Facebook posts showed that anti-vaccine groups were discussing COVID-19 in the first week of February 2020 and were specifically discussing COVID-19 vaccines by mid-February 2020. COVID-19 posts by NVIC were more widely disseminated and showed greater influence than non-COVID-19 posts. Early COVID-19 posts concerned mistrust of vaccine safety and conspiracy theories.

Conclusion
Major anti-vaccine groups were sowing seeds of doubt on Facebook weeks before the US government launched its vaccine development program ‘Operation Warp Speed’. Early anti-vaccine misinformation campaigns outpaced public health messaging and hampered the rollout of COVID-19 vaccines.
Evaluating dietary acidifiers as alternatives for conventional feed-based antibiotics in nursery pig diets
Authors: Dahmer Payton L , Jones Cassandra K 
Published: March 2021

AbstractA total of 360 weanling pigs (DNA 200 × 400; initially 9.7 ± 0.23 kg BW) were used in a 21-d experiment with 6 pigs/pen, 10 replicate pens/treatment, and 2 separate nursery rooms, each with 30 pens. Pigs were weighed and allotted to pens based on BW in a completely randomized block design to one of six treatment diets: 1) Negative control (no organic acids or antibiotics) and the control with 2) 0.25% acidifier A; 3) 0.3% acidifier B; 4) 0.5% acidifier C); 5) 50 g/ton carbadox; and 6) 400 g/ton chlortetracycline (CTC). Upon weaning, a common diet with no antibiotics or additives was fed for 21 d (Phases 1 and 2; days −21 to 0), followed by a 21-d experimental period (Phase 3; days 0 to 21) where treatment diets were fed. Pigs and feeders were individually weighed on a weekly basis to calculate average daily gain (ADG), average daily feed intake (ADFI), and feed efficiency (G:F). Data were analyzed using the PROC GLIMMIX procedure of SAS (v 9.4, SAS Inst., Cary, NC) with pen as the experimental unit, treatment as a fixed effect, and room as a random effect. Dietary treatment had a significant impact (P &lt; 0.05) on ADG, ADFI, and G:F each week and for the overall experimental period (days 0 to 21). Specifically, from days 0 to 7, pigs fed CTC had increased (P = 0.001) ADG compared with those fed acidifier B, acidifier C, and carbadox, whereas pigs fed the negative control and acidifier A diets were intermediate. Additionally, pigs fed the CTC diet had improved (P = 0.0002) ADFI when compared with all other treatments. From days 7 to 14 and days 14 to 21, pigs fed the carbadox diet had decreased (P &lt; 0.0001) ADG compared with all other treatments. During the overall period (days 0 to 21), pigs fed diets containing carbadox had reduced ADG and ADFI (P &lt; 0.0001), whereas pigs fed CTC had improved (P &lt; 0.0001) ADG compared with all other treatments. Additionally, blood parameters, fecal consistency, and fecal microbial populations were analyzed on a subset of pigs (n = 5 pigs/treatment). Dietary treatment significantly affected (P &lt; 0.05) concentrations of protein, globulin, phosphorus, alkaline phosphatase, and sorbitol dehydrogenase in the blood. Treatment also significantly impacted (P = 0.0005) fecal score but did not affect (P = 0.59) fecal microbial growth from days 0 to 21. In summary, CTC continues to be a valuable additive to improve performance in the nursery. Further investigation surrounding the efficacy of dietary acidifiers as antibiotic alternatives is warranted given inconclusive evidence in this study.
Comparative genomic analysis of genogroup 1 and genogroup 2 rotaviruses circulating in seven US cities, 2014–2016
Authors: Esona Mathew D , Gautam Rashi , Katz Eric , Jaime Jose , Ward M Leanne , Wikswo Mary E , Betrapally Naga S , Rustempasic Slavica M , Selvarangan Rangaraj , Harrison Christopher J , Boom Julie A , Englund Jan , Klein Eileen J , Staat Mary Allen , McNeal Monica M , Halasa Natasha , Chappell James , Weinberg Geoffrey A , Payne Daniel C , Parashar Umesh D , Bowen Michael D 
Published: March 2021

AbstractFor over a decade, the New Vaccine Surveillance Network (NVSN) has conducted active rotavirus (RVA) strain surveillance in the USA. The evolution of RVA in the post-vaccine introduction era and the possible effects of vaccine pressure on contemporary circulating strains in the USA are still under investigation. Here, we report the whole-gene characterization (eleven ORFs) for 157 RVA strains collected at seven NVSN sites during the 2014 through 2016 seasons. The sequenced strains included 52 G1P[8], 47 G12P[8], 18 G9P[8], 24 G2P[4], 5 G3P[6], as well as 7 vaccine strains, a single mixed strain (G9G12P[8]), and 3 less common strains. The majority of the single and mixed strains possessed a Wa-like backbone with consensus genotype constellation of G1/G3/G9/G12-P[8]-I1-R1-C1-M1-A1-N1-T1-E1-H1, while the G2P[4], G3P[6], and G2P[8] strains displayed a DS-1-like genetic backbone with consensus constellation of G2/G3-P[4]/P[6]/P[8]-I2-R2-C2-M2-A2-N2-T2-E2-H2. Two intergenogroup reassortant G1P[8] strains were detected that appear to be progenies of reassortment events between Wa-like G1P[8] and DS-1-like G2P[4] strains. Two Rotarix® vaccine (RV1) and two RV5 derived (vd) reassortant strains were detected. Phylogenetic and similarity matrices analysis revealed 2–11 sub-genotypic allelic clusters among the genes of Wa- and DS-1-like strains. Most study strains clustered into previously defined alleles. Amino acid (AA) substitutions occurring in the neutralization epitopes of the VP7 and VP4 proteins characterized in this study were mostly neutral in nature, suggesting that these RVA proteins were possibly under strong negative or purifying selection in order to maintain competent and actual functionality, but fourteen radical (AA changes that occur between groups) AA substitutions were noted that may allow RVA strains to gain a selective advantage through immune escape. The tracking of RVA strains at the sub-genotypic allele constellation level will enhance our understanding of RVA evolution under vaccine pressure, help identify possible mechanisms of immune escape, and provide valuable information for formulation of future RVA vaccines.