bluuebunny/crossref_metadata_2025_split huggingface dataset:
Investigation of the properties and activity of DfCas9 and DsCas9 nucleases in eucaryotic cells
Authors: Vlasova Yelizaveta V. , Madera Dmitry A. , Gershovich Pavel M. 
Published: December 2021

This study is focused on the two novel nucleases of the CRISPR/Cas9 family, which were found in bacterial genomes of DfCas9 (Defluviimonas sp) и DsCas9 (Demequina sediminicola). Discovery of these nucleases was part of the results of a joint study conducted by BIOCAD together with Skoltech Institute of Science and Technology and Saint-Petersburg Polytechnical University (SPPU) under a grant agreement with the Department of Science and Education of Russian Federation (Agreement number 14.606.21.0006 from September, 26th 2017). Under the agreement the nucleases DfCas9 and DsCas9 were characterized in vitro by Skoltech and SPPU.
Based on the aforementioned results, in this study we characterized the genome-modifying nuclease activity of these enzymes in a mammalian cell line HEK293. Specifically, we created genetic constructs designed to express the nucleases DsCas9 and DfCas9 together with the necessary guide RNA molecules (sequences of the guide RNAs were described previously) [1]. We demonstrated expression of the nucleases on a protein level, as well as activity of DfCas9 at the VEGF2 locus in HEK293 cells. The theoretical study was conducted by analyzing international and national literature. The experimental part was performed with a restriction-ligation cloning method, transient transfections, Western blot protein detection method, and a T7 nuclease-based method of detection of heteroduplex double-stranded DNA.
Clinico-pathological correlation of linear dermatoses along the lines of Blaschko: An observational study
Authors: Pise Gajanan A , Dastikop Shilpa V , S Manjunath , Manohar Naveen , Patil Malteshgauda N 
Published: December 2021

Several epidemiological studies have described linear dermatoses; however, only few studies have correlated linear dermatoses along Blaschko’s lines with the histopathological findings. The aims of this study were to investigate the clinical patterns of various linear dermatological lesions along Blaschko’s lines and investigate the correlations between these dermatological lesions and their histopathological features. Patients who attended our dermatology out-patient department with linear dermatoses along the Blaschko’s lines were included in the study. Detailed history was obtained, clinical examination was performed, and a final provisional clinical diagnosis was noted. Subsequently, biopsy of the lesions was performed for histopathological examination. Of 62 patients who presented with linear lesions along the Blaschko’s lines, 45 patients underwent biopsy and histopathological examination. Of 45 patients who underwent biopsy, clinico-pathological correlation was observed in 100% of those diagnosed with lichen striatus and linear morphea, 89% of those diagnosed with hypomelanosis of Ito, 80% of those diagnosed with linear epidermal nevus, 75% of those diagnosed with lichen planus, and 67% of those diagnosed with nevus depigmentosus. The importance of correlation of linear lesions with their histopathological features in dermatology cannot be over emphasized. However, 100% correlation may be wishful thinking, and a correlation can help choose the appropriate line of management. Our results highlight this discrepancy and add to the knowledge on linear dermatosis.
Capillaroscopic nail fold changes in collagen vascular diseases: A prospective observational study from a tertiary care teaching hospital in Western India
Authors: Shukla Ratnakar , Kapoor Sheena , Patil Sharmila , Patil Anant 
Published: December 2021

: To examine nail changes with capillaroscopy in patients with collagen vascular diseases. In this prospective observational study, patients with collagen vascular diseases presented to dermatology outpatient department of a tertiary care center were subjected to capillaroscopy. Structural and morphological abnormalities in these patients were noted. Fifty patients (Mean age 38.2 years; 86% females) with mean disease duration of 1.96 years were included. Patients with systemic sclerosis and systemic lupus erythematosus constituted 34% and 32% patients respectively. Twenty two (44%) patients had presence of dilated nail fold capillary loops. Out of 17 patients with systemic sclerosis, 15(88.23%) and 14(87.5%) showed dilated nail fold capillary loops and capillary drop-pouts respectively. Capillary dropouts were observed in 14(82.35%) patients. Raynaud’s phenomenon, splinter hemorrhage and fingertip scarring was observed in 11(64.71%), six (35.29%) and 33(17.65%) patients respectively. Ragged cuticle was observed in four (25%) patients with systemic lupus erythematosus. In our study, systemic sclerosis and systemic lupus erythematosus were two most common disorders. Dilated nail fold capillary loops/giant capillaries were the most common observation in overall population. Dilated nail fold capillary loops, capillary dropouts, Raynaud’s phenomenon, splinter hemorrhage and fingertip scarring were common in patients with systemic sclerosis.
Analisis Sidik Jari Kromatografi Lapis Tipis Tanaman Anting-Anting (Acalypha indica L.)
Authors: Laksono Muhammad Teguh , Hayati Elok Kamilah 
Published: December 2021

Anting-anting plant (Acalypha indica L.) is a plant that the community has used as raw material for herbal medicine. Anting-anting plants contain chemical compounds, so it is difficult to ensure these plants' safety and quality control. The research objective was to validate the method by knowing the stability, specificity, precision, and rigidity of the chromatographic profile of alkaloid compounds in the TLC analysis of the anting-anting extract. Extraction was carried out using ethyl acetate solvent for 20 minutes with a frequency of 42 kHz. The eluent used is cyclohexane: toluene: diethylamine (75:15:10) with Dragendorff reagent. The method of validation was acceptable according to the criteria except for the stability test of the analyte during chromatography. The instability of the analyte is indicated that not all spots form a straight diagonal line. The specificity of the method was carried out by comparing the fingerprint patterns of the leaves of the anting-anting with ekor kucing (Acalypha hibsida Farm.) and teh-tehan plants (Acalypha siamensis). The fingerprint profile showed a yellow stain (Rf = 0.39) on the leaves of the anting-anting, which did not appear in the fingerprint profiles of the two comparison samples. Keywords: anting-anting, fingerprint analysis, thin layer chromatography, method validation Anting-anting (Acalypha indica L.) merupakan tanaman yang telah dimanfaatkan masyarakat sebagai bahan baku obat herbal. Anting-anting mengandung banyak senyawa kimia sehingga sulit menjamin keamanan dan pengendalian mutu dari tanaman tersebut. Penelitian ini dilakukan untuk validasi metode dengan mengetahui kestabilan, spesifitas, presisi, dan ketegaran profil kromatografi senyawa alkaloid pada analisis KLT ekstrak anting-anting. Ekstraksi dilakukan menggunakan pelarut etil asetat selama 20 menit dengan frekuensi 42 kHz. Eluen yang digunakan adalah sikloheksana : toluena : dietilamin (75:15:10) dengan reagen Dragendorff. Validasi metode dapat diterima sesuai kriteria kecuali uji stabilitas analit selama kromatografi. Ketidakstabilan analit ditunjukkan dengan seluruh noda yang dihasilkan tidak membentuk garis diagonal yang lurus. Spesifitas pada metode dilakukan dengan membandingkan pola sidik jari daun anting-anting dengan daun ekor kucing (Acalypha hibsida Farm) dan daun teh-tehan (Acalypha siamensis). Profil sidik jari menunjukkan bahwa terdapat noda berwarna kuning kehijauan (Rf = 0,39) pada daun anting-anting yang tidak muncul pada profil sidik jari kedua sampel pembanding tersebut. Kata kunci: anting-anting, analisis sidik jari, kromatografi lapis tipis, validasi metode
An expert system for predicting the infiltration characteristics
Authors: Singh Balraj , Ebtehaj Isa , Sihag Parveen , Bonakdari Hossein 
Published: December 2021

Abstract
Infiltration plays a fundamental role in streamflow, groundwater recharge, subsurface flow, and surface and subsurface water quality and quantity. This study includes a comparative analysis of the two machine learning techniques, M5P model tree (M5P) and Gene Expression Programming (GEP), in predictions of the infiltration characteristics. The models were trained and tested using the 7 combination (CMB1 – CMB7) of input parameters; moisture content (m), bulk density of soil (D), percentage of silt (SI), sand (SA) and clay (C), and time (t), with output parameters; cumulative infiltration (CI) and infiltration rate (IR). Results suggested that GEP has an edge over M5P to predict the IR and CI with R, RMSE and MAE values 0.9343, 15.9667 mm/hr & 8.7676 mm/hr, and 0.9586, 9.2522 mm and 7.7865 mm for IR and CI, respectively with CMB1. Although the M5P model also gave good results with R, RMSE and MAE values 0.9192, 14.1821 mm/hr, and 19.2497 mm/hr, and 0.8987, 11.2144 mm and 18.4328 mm for IR and CI, respectively, but lower than GEP. Furthermore, single-factor ANOVA and uncertainty analysis were used to show the significance of the predicted results and to find the most efficient soft computing techniques respectively.
„Causa simulandi” a wykluczenie dobra wierności małżeńskiej
Authors: Leszczyński Grzegorz 
Published: December 2021

Zarówno doktryna, jak i orzecznictwo wskazują, że wykluczenie dobra wierności musi dokonać się pozytywnym aktem woli, jednak przydatne w określeniu prawdziwej woli symulanta zdaje się być określenie relacji zachodzącej między wolą zawarcia małżeństwa a wolą wykluczenia dobra wierności. Ważne jest określenie tych przyczyn, zarówno dalszych, jak i bliższych. Orzecznictwo wskazuje nie tylko na zaburzenia czy anomalie psychoseksualne, ale w obecnym czasie znaczenie posiada przede wszystkim mentalność współczesnych ludzi, propagujących idee wolności seksualnej, które w niektórych przypadkach mogą osiągać znamiona osobowości zdeprawowanej. Przyczyny bliższe są bardzo różne w zależności od przypadku, choć do najczęstszych zalicza się chęć utrzymania relacji pozamałżeńskiej z osobą, z którą kontrahent był związany przed zawarciem małżeństwa.
Convolutional Neural Network–Based Automatic Classification of Colorectal and Prostate Tumor Biopsies Using Multispectral Imagery: System Development Study
Authors: Peyret Remy , alSaeed Duaa , Khelifi Fouad , Al-Ghreimil Nadia , Al-Baity Heyam , Bouridane Ahmed 
Published: December 2021

Background
Colorectal and prostate cancers are the most common types of cancer in men worldwide. To diagnose colorectal and prostate cancer, a pathologist performs a histological analysis on needle biopsy samples. This manual process is time-consuming and error-prone, resulting in high intra- and interobserver variability, which affects diagnosis reliability.

Objective
This study aims to develop an automatic computerized system for diagnosing colorectal and prostate tumors by using images of biopsy samples to reduce time and diagnosis error rates associated with human analysis.

Methods
In this study, we proposed a convolutional neural network (CNN) model for classifying colorectal and prostate tumors from multispectral images of biopsy samples. The key idea was to remove the last block of the convolutional layers and halve the number of filters per layer.

Results
Our results showed excellent performance, with an average test accuracy of 99.8% and 99.5% for the prostate and colorectal data sets, respectively. The system showed excellent performance when compared with pretrained CNNs and other classification methods, as it avoids the preprocessing phase while using a single CNN model for the whole classification task. Overall, the proposed CNN architecture was globally the best-performing system for classifying colorectal and prostate tumor images.

Conclusions
The proposed CNN architecture was detailed and compared with previously trained network models used as feature extractors. These CNNs were also compared with other classification techniques. As opposed to pretrained CNNs and other classification approaches, the proposed CNN yielded excellent results. The computational complexity of the CNNs was also investigated, and it was shown that the proposed CNN is better at classifying images than pretrained networks because it does not require preprocessing. Thus, the overall analysis was that the proposed CNN architecture was globally the best-performing system for classifying colorectal and prostate tumor images.
Acceptability of Serious Games in Pediatric Asthma Education and Self-management: Pilot Study
Authors: Silva-Lavigne Nicole , Valderrama Alena , Pelaez Sandra , Bransi Myriam , Balli Fabio , Gervais Yannick , Gaudy Thomas , Tse Sze Man 
Published: December 2021

Background
Asthma is the most common chronic pediatric disease. Despite existing tools to manage asthma, 40%-55% of children with asthma experience uncontrolled asthma. Serious games (SGs) represent a novel approach in promoting asthma education and self-management for children.

Objective
In this qualitative pilot study with an embedded quantitative design, we aim to use focus groups and questionnaires to describe the perceived role of SGs in different aspects of asthma self-management by children and their parents. These aspects include asthma perception and knowledge, the impact of asthma and barriers to asthma self-management, and the support system for asthma self-management.

Methods
A total of 5 children with asthma and their parents were invited to participate in an organized gaming session. Children and their parents completed a pregaming questionnaire on their medical history and asthma knowledge. Then, they were invited to test 4 original SG prototypes, after which the children answered a postgaming questionnaire on their asthma knowledge and perception of the SGs. Children and their parents subsequently participated in parallel focus groups, which were video-recorded or audio-recorded, transcribed verbatim, and analyzed by reaching consensus among members of the research team.

Results
The mean age of the children was 10.3 (SD 1.5) years, with 20% (1/5) of the children being male. Qualitative data from the transcripts were coded into three separate domains: asthma self-management perception and knowledge, impact of asthma and barriers to asthma self-management, and support system for asthma self-management. We specifically explored the perceived roles of SGs within each domain. A key takeaway message was identified for each of these three domains: heterogeneity of asthma knowledge and the ability of SGs to encourage knowledge transfer through games, consequences and limitations of asthma and the ability of SGs to allow for identification and management of real-life situations through games, and insufficient support system and the ability of SGs to encourage playing with others for support and shared knowledge.

Conclusions
Our pilot study explored the role of SGs in the self-management of asthma, as perceived by children and their parents. Our findings support the acceptability of SGs in asthma education and self-management in pediatrics and the necessity for future development in this field.
La canasta de consumo de los hogares dominicanos, su evolución histórica y los posibles cambios derivados de la pandemia del COVID-19
Authors: Santana Isidoro 
Published: December 2021

Este artículo muestra la estructura de consumo de la población dominicana antes de la pandemia, a partir de datos de la ENGIH-2018, los cambios de la canasta desde la primera ENGIH en 1976-1977 y, finalmente, se emite una idea de los posibles efectos de la pandemia sobre esta. En la estructura de consumo familiar influyen factores como nivel de ingresos, urbanización, tamaño de los hogares, estratificación social, educación, etc. A su vez, en una economía bastante cerrada, la estructura de consumo es determinante de las estructuras de producción, empleo, comercio, ingresos fiscales, comercio exterior y condiciones sociales. Los hábitos de gastos cambian con el tiempo y las circunstancias. En medio siglo, la economía dominicana ha crecido, han surgido nuevos sectores y la población se ha urbanizado. Con esto, ha cambiado la canasta hacia una menor ponderación del componente alimentación y de prendas de vestir, y el consiguiente incremento del consumo de servicios, incluyendo transporte, educación, salud y comunicaciones. Dentro de la alimentación, han ganado terreno los alimentos servidos fuera del hogar. La pandemia del COVID-19 impactó de manera aguda los modos de vida y es previsible que los hábitos de consumo de la gente, impactando a su vez la estructura productiva y de empleo.
Reflexión teórica de la asociatividad de las Mipymes como mecanismo de impacto en el cumplimiento de los ODS (Objetivos de desarrollo sostenible)
Authors: Sanabria Martínez Martha Janneth , Banda Mora Ana del Pilar 
Published: December 2021

El impacto de las acciones que desarrolla el sector empresarial desde el componente económico al mantener la rentabilidad de los negocios y, desde el componente social al generar empleo, capacitar y formar al recurso humano, permite mejorar la competitividad y la innovación de los productos, en el marco de los ODS.
El estudio partió de la pregunta problema ¿Cómo las Mypimes en Colombia podrán optimizar sus vínculos en el marco del cumplimiento de los ODS?, siendo la finalidad el identificar, caracterizar y triangular información a partir de los marcos teóricos y conceptuales desde diferentes ópticas, contrastes y momentos históricos.
La construcción teórica evidenció la asociatividad como la forma de integración que consolida la cultura como estrategia adaptativa, en los componentes económico, social y ambiental, porque no es solo generar rentabilidad, sino el desarrollo humano, la preservación y conservación del patrimonio natural. Permitiendo la consolidación de estructuras sólidas y competitivas y, el fortalecimiento de las ventajas entre las empresas, las relaciones y mejorar el entorno, logrando mayor productividad y dinámica empresarial al crearse y consolidarse cadenas productivas con objetivos comunes y prácticas responsables.
O USO DE TECNOLOGIAS DIGITAIS NO PROCESSO DE APRENDIZAGEM NO ENSINO REMOTO: UMA REFLEXÃO NECESSÁRIA
Authors: Vieira da Silva Mônica , Carvalho Edione Teixeira de 
Published: December 2021

Com uma sociedade cada vez mais tecnológica, a educação precisa estar atenta e acompanhar essas mudanças. Refletir sobre os impactos que a inserção tecnológica causa no contexto educacional é o objetivo desse trabalho. Através de uma pesquisa bibliográfica, de natureza aplicada, com abordagem qualitativa e exploratória, o estudo de caso utilizou um questionário aberto e observações das práticas docentes como instrumentos de coleta de dados. A pesquisa realizada com 14 professores da Escola Estadual Conquista D’Oeste evidenciou o porquê de os professores resistirem tanto a essa nova realidade e como a formação continuada pode ajudar a quebrar essa resistência. Também revela a importância da inserção das Tecnologias de Informação na educação, observando as necessidades dos educandos, favorecendo a integração dos estudantes no processo de construção do conhecimento, uma vez que eles já crescem informatizados, o que torna imprescindível para a educação a qualificação de seus profissionais.
PERBEDAAN KELAS SOSIAL DALAM PAKET MANJOLAG SUAGHANG: PRAKTEK BUDAYA ORANG OCU (Studi Antropologi Sosial Budaya)
Authors: Syafrizal Syafrizal 
Published: December 2021

Tujuan penguraian artikel ini adalah untuk menjelaskan fenomena Manjolang Suaghang dalam praktek budaya orang Ocu. Praktek Manjolang Suaghang merupakan salah satu aspek budaya orang Ocu yang khas dan belum banyak dikenal oleh masyarakat pada umumnya. Sebagai budaya khas dari masyarakat orang Ocu, praktek Manjolang Suaghang selain dilaksanakan secara bersamaan dalam satu paket kegiatan budaya, namun sebenarnya masing-masing memiliki kandungan makna yang berbeda. Manjolang memiliki substansi praktek budaya memberikan penghambaan atau penghormatan kepada keluarga pihak perempuan terutama kepada abang atau adik dari pihak ibu perempuan, yang secara adat adalah orang yang memiliki andil dan kuasa untuk memberikan Suaghang kepada laki-laki yang baru beberapa waktu sebagai orang baru dikeluarga pihak perempuan yang disebut dengan istilah menantu. Manjolang dimaknakan sebagai proses penghambaan dan Suaghang dimaknai sebagai penyerahan atau pemberian sebagai akibat status baru seorang laki-laki yang masuk ke dalam keluarga perempuan sebagai suami atau menatu. Praktek budaya ini biasanya dilakukan selang beberapa hari setelah dilangsungkannya sebuah perkawinan, dan bukanlah satu proses yang menyatu dalam keseluruhan tahapan perkawinan dalam masyarakat orang Ocu, akan tetapi murni sebagai salah satu bentuk penghargaan yang sangat tinggi terhadap seorang laki-laki baru yang akan hidup bersama dalam keluarga pihak perempuan dengan status menantu. Dalam realitasnya praktek budaya ini tidak semua orang Ocu keluarga orang mampu untuk melakukannya, akan tetapi sangat dipengaruhi oleh perbedaan kelas sosial. Artikel ini merupakan hasil penelitian, menggunakan pendekatan kualitatif, dengan melakukan wawancara mendalam, kepada nara sumber terpilih. Adapun lokasi pelaksanaan penelitian ini adalah Di Kenegarian Rumbio Lama, Kecamatan Kampar Propinsi Riau, dengan jangka waktu lebih kurang selama 7 (tujuh bulan), antara bulan Desember 2016 sampai dengan bulan Juni 2017. Hasil penganalisaan terhadap data didapatkan bahwa praktek Manjolang Suaghang sebagai praktek budaya lokal (kearifan lokal) masyarakat orang Ocu, ternyata memiliki sisi-sisinya yang unik, yaitu sejak awal sudah tercium perbedaan kelas sosialnya dan tidak jarang akan bermetamorposis menjadi bibit konflik dalam keluarga perempuan sekaligus penyebab retaknya suatu perkawinan.
JEJAK ISLAM DI KOTA BINJAI 1887-1956
Authors: Tanjung Yushar , Sembiring Janita Anggraini 
Published: December 2021

Penelitian ini bertujuan untuk mengetahui proses awal masuknya agama Islam di Kota Binjai, untuk mengetahui perkembangan agama Islam di Kota Binjai, untuk mengetahui tokoh-tokoh yang berpengaruh dalam perkembangan agama Islam di Kota Binjai, untuk mengetahui bangunan-bangunan peninggalan bercorak Islam di Kota Binjai dan untuk mengetahui jejak-jejak Islam yang terdapat di kota Binjai dan masih terdapat peninggalannya hingga sekarang . Penelitian yang dilakukan adalah penelitian dengan menggunakan metode Library Research (metode penelitian kepustakaan) dan Field Research Methode ( metode penelitian lapangan) dengan menggunakan pendekatan deskriptif historis dan teknik yang digunakan studi pustaka dan wawancara. Teknik pengumpulan data yang dilakukan dengan mengumpulkan dokumentasi atau memfoto peninggalan-peninggalan jejak Islam di Kota Binjai. Hasil yang diperoleh dari penelitian penyebaran Islam di Kota Binjai ini tidak terlepas dari peran yang besar dalam penyebaran agama Islam dari Kesultanan Langkat yang berkedudukan di Kota Binjai karena Binjai merupakan bagian dari kesultanan langkat sebelum akhirnya membentuk daerahnya sendiri. Dan juga terdapat peninggalan-peninggalan Islam di Kota Binjai yang berupa masjid, makam, sekolah Islam dan Ulama.