bluuebunny/crossref_metadata_2025_split huggingface dataset:
Intensive care doctors and nurses personal preferences for Intensive Care, as compared to the general population: a discrete choice experiment
Authors: Anstey Matthew H. , Mitchell Imogen A. , Corke Charlie , Murray Lauren , Mitchell Marion , Udy Andrew , Sarode Vineet , Nguyen Nhi , Flower Oliver , Ho Kwok M. , Litton Edward , Wibrow Bradley , Norman Richard 
Published: August 2021

Abstract
Background
To test the hypothesis that Intensive Care Unit (ICU) doctors and nurses differ in their personal preferences for treatment from the general population, and whether doctors and nurses make different choices when thinking about themselves, as compared to when they are treating a patient.

Methods
Cross sectional, observational study conducted in 13 ICUs in Australia in 2017 using a discrete choice experiment survey. Respondents completed a series of choice sets, based on hypothetical situations which varied in the severity or likelihood of: death, cognitive impairment, need for prolonged treatment, need for assistance with care or requiring residential care.

Results
A total of 980 ICU staff (233 doctors and 747 nurses) participated in the study. ICU staff place the highest value on avoiding ending up in a dependent state. The ICU staff were more likely to choose to discontinue therapy when the prognosis was worse, compared with the general population. There was consensus between ICU staff personal views and the treatment pathway likely to be followed in 69% of the choices considered by nurses and 70% of those faced by doctors. In 27% (1614/5945 responses) of the nurses and 23% of the doctors (435/1870 responses), they felt that aggressive treatment would be continued for the hypothetical patient but they would not want that for themselves.

Conclusion
The likelihood of returning to independence (or not requiring care assistance) was reported as the most important factor for ICU staff (and the general population) in deciding whether to receive ongoing treatments. Goals of care discussions should focus on this, over likelihood of survival.
SCAPTURE: a deep learning-embedded pipeline that captures polyadenylation information from 3′ tag-based RNA-seq of single cells
Authors: Li Guo-Wei , Nan Fang , Yuan Guo-Hua , Liu Chu-Xiao , Liu Xindong , Chen Ling-Ling , Tian Bin , Yang Li 
Published: August 2021

AbstractSingle-cell RNA-seq (scRNA-seq) profiles gene expression with high resolution. Here, we develop a stepwise computational method-called SCAPTURE to identify, evaluate, and quantify cleavage and polyadenylation sites (PASs) from 3′ tag-based scRNA-seq. SCAPTURE detects PASs de novo in single cells with high sensitivity and accuracy, enabling detection of previously unannotated PASs. Quantified alternative PAS transcripts refine cell identity analysis beyond gene expression, enriching information extracted from scRNA-seq data. Using SCAPTURE, we show changes of PAS usage in PBMCs from infected versus healthy individuals at single-cell resolution.
Clinical value and role of microRNA-29c-3p in sepsis-induced inflammation and cardiac dysfunction
Authors: Zhang Bingyu , Yu Lin , Sheng Ying 
Published: August 2021

Abstract
Background
The goal of this study was to investigate the diagnostic value of miR-29c-3p in sepsis and its role in sepsis-induced inflammatory response and cardiac dysfunction.

Methods
Serum level of miR-29c-3p was detected by qRT-PCR. The ROC curve was used to evaluate the diagnostic value of miR-29c-3p for Sepsis. The cecal ligation and puncture method (CLP) was used to establish a rat sepsis model. To assess cardiac function, left ventricular systolic pressure (LVSP), left ventricular end diastolic pressure (LVEDP) and maximum rate of rise/fall of left ventricle pressure (± dp/dtmax) in different experimental groups were detected, and the serum cardiac troponin I (cTnI), creative kinase isoenzyme MB (CK-MB) were measured by ELISA. Meanwhile, TNF-α, IL-1β, and IL-6 were detected by ELISA to assess the level of inflammatory response in animals.

Results
miR-29c-3p level was upregulated in sepsis patients. ROC curve revealed that miR-29c-3p had the ability to distinguish sepsis patients from healthy controls. Cardiac dysfunction and inflammation were observed in sepsis rat, which were characterized by the decrease of LVSP and + dp/dtmax, the increase of LVEDP, − dp/dtmax, cTnI, CK-MB, TNF-α, IL-1β, IL-6. All effects were reversed by the injection of miR-29c-3p antagomir. Logistics regression analysis manifested miR-29c-3p is an independent factor in the occurrence of cardiac dysfunction in sepsis patients.

Conclusions
miR-29c-3p has potential as a biomarker for the diagnosis of sepsis, and inhibition of miR-29c-3p expression in animal models reduced sepsis-induced cardiac dysfunction and inflammatory response.
Forest management required for consistent carbon sink in China’s forest plantations
Authors: Yu Zhen , You Weibin , Agathokleous Evgenios , Zhou Guoyi , Liu Shirong 
Published: August 2021

Abstract
Background
Forest is the largest biomass carbon (C) pool in China, taking up a substantial amount of atmospheric carbon dioxide. Although it is well understood that planted forests (PFs) act as a large C sink, the contribution of human management to C storage enhancement remains obscure. Moreover, existing projections of forest C dynamics suffer from spatially inconsistent age and type information or neglected human management impacts. In this study, using developed PF age and type maps and data collected from 1371 forest plantation sites in China, we simulated biomass C stock change and quantified management impacts for the time period 2010–2050.

Results
Results show that future forest biomass C increment might have been overestimated by 32.5%–107.5% in former studies. We also found that age-related growth will be by far the largest contributor to PF biomass C increment from 2010 to 2050 (1.23 ± 0.002 Pg C, 1 Pg = 1015 g = 1 billion metric tons), followed by the impact of human management (0.57 ± 0.02 Pg C), while the contribution of climate is slight (0.087 ± 0.04 Pg C). Besides, an additional 0.24 ± 0.07 Pg C can be stored if current PFs are all managed by 2050, resulting in a total increase of 2.13 ± 0.05 Pg C.

Conclusions
Forest management and age-related growth dominate the biomass C change in PFs, while the effect of climatic factors on the accumulation is minor. To achieve the ambitious goal of forest C stock enhancement by 3.5 Pg from 2020 to 2050, we advocate to improve the management of existing forests and reduce the requests for more lands for forest expansion, which helps mitigate potential conflicts with agricultural sectors. Our results highlight that appropriate planning and management are required for sustaining and enhancing biomass C sequestration in China’s PF.
A qualitative study on the impact of caring for an ambulatory individual with nonsense mutation Duchenne muscular dystrophy
Authors: Williams Kate , Davidson Ian , Rance Mark , Buesch Katharina , Acaster Sarah 
Published: August 2021

Abstract
Background
Duchenne muscular dystrophy is a rare genetic neuromuscular disorder, which can result in early death due to disease progression. Ataluren is indicated for the treatment of nonsense mutation Duchenne muscular dystrophy, in ambulatory individuals aged two years and older. This study explored the impact of caring for an ambulatory individual with nonsense mutation Duchenne muscular dystrophy, as well as the impact of treatment with ataluren on the caregiver experience, using retrospective recall.

Methods
Qualitative interviews were conducted with caregivers in the UK. Interviews were conducted by telephone, were recorded and transcribed. Data were analysed using thematic analysis and saturation was recorded.

Results
Ten interviews were conducted with parents of individuals aged 4–19 years. Caregivers reported proximal impacts (physical, emotional, time-related), and distal impacts (work, relationships, social life) of caring for their sons. The relationships between these impacts were illustrated in a conceptual model. Changes to the caregiver experience since initiation with their son’s treatment were discussed.

Conclusion
Caring for an ambulatory individual with nonsense mutation Duchenne muscular dystrophy has a substantial multifaceted impact on caregivers. Treatments which have the potential to improve symptoms or delay progression, may also have a positive impact on the quality of life of caregivers.
Advanced image guidance for prostatic artery embolization – a multicenter technical note
Authors: Carnevale Francisco Cesar , McClure Timothy , Cadour Farah , Vidal Vincent , de Assis André Moreira , Moreira Airton Mota , Rocha Arthur Diego Dias , Rebet Aya , Nutting Charles 
Published: August 2021

Abstract
Background
Prostatic artery embolization (PAE) is associated with patients’ quality of life improvements and limited side effects compared to surgery. However, this procedure remains technically challenging due to complex vasculature, anatomical variations and small arteries, inducing long procedure times and high radiation exposure levels both to patients and medical staff. Moreover, the risk of non-target embolization can lead to relevant complications. In this context, advanced imaging can constitute a solid ally to address these challenges and deliver good clinical outcomes at acceptable radiation levels.

Main text
This technical note aims to share the consolidated experience of four institutions detailing their optimized workflow using advanced image guidance, discussing variants, and sharing their best practices to reach a consensus standardized imaging workflow for PAE procedure, as well as pre and post-operative imaging.

Conclusions
This technical note puts forth a consensus optimized imaging workflow and best practices, with the hope of helping drive adoption of the procedure, deliver good clinical outcomes, and minimize radiation dose levels and contrast media injections while making PAE procedures shorter and safer.
The exchange of Musa spp. fibre in composite fabrication: a systematic review
Authors: Odera Raphael Stone , Okechukwu Onukwuli Dominic , Ezeh Ernest Mbamalu , Menkiti Mathew Chukwudi , Agu Peter Chinedu 
Published: August 2021

AbstractBackgroundThe areas of application of natural fibres have gained popularity in recent times due to their attractive advantages when compared with other materials of engineering. These advantages include lightness, cost-effectiveness, and ease of processing, ecological friendliness, and durability. Previously, farmers only harvestMusaspp. fruits for their food values and packaging purposes.Main body of the abstractSeveral research works have been undertaken which accentuate the applications of the assumed waste portions ofMusaspp. (banana and plantain) specificallyMusaspp. fibre as a reinforcement material in composite manufacture. As a material for reinforcement in composites, the characterization, treatment, and fabrication techniques; elemental, chemical, and mechanical properties ofMusaspp. fibre have been analysed. The mechanical properties of banana fibre reinforcement in polyester, epoxy, cement, and plastics composites were evaluated with those of other biodegradable fibres to explicate their relationships.Short conclusionThis review aims to explore the current state of knowledge on the interaction of Musaspp. fibre in composite manufacture, to aid intending researchers with ample knowledge on the choice of material in bio-based composite design.
Cultural motives affecting tea purchase behavior under two usage situations in China: a study of renqing, mianzi, collectivism, and man-nature unity culture
Authors: Tong Lingyun , Toppinen Anne , Wang Lei 
Published: August 2021

AbstractTea should be perceived more as a cultural product than a fast-moving consumer good with its roots deep in different cultures across nations. Nevertheless, consumer demands on tea have not been explored sufficiently from a cultural perspective. This research aims to examine motives that are driving Chinese tea consumption on two usage occasions, under the influence of the Chineserenqing,mianzi, collectivism, and man-nature unity culture. Linkage was established between Chinese culture and consumer motives. The factor analysis on 280 respondents identified five consumer psychological motives. Further logistic regression analysis suggested consumer motives and usage situations were related. The contribution of the findings is summarized. On one hand, this study adds to the limited understandings on consumer motives of tea in an emerging country from the cultural aspect. On the other hand, deep understandings of brand chasing, sustainability, and pragmatism motives should businesses to better serve consumers.
A genetic variant of the NAMPT gene rs4730153 as a risk factor for the metabolic syndrome in younger age: a single-centre pilot study in Yogyakarta, Indonesia
Authors: Puspasari Anggelia , Hastuti Pramudji , Sadewa Ahmad Hamim , Mus Rosdiana , Maharani Citra , Setyawati Ika 
Published: August 2021

AbstractBackgroundThe genetic variation ofnicotinamide phosphoribosyl transferase(NAMPT) gene rs4730153 is reported to be associated with cardiometabolic risk, but the results are inconsistent between populations. Ethnicity, metabolic risk and lifestyle play a role in the association of the genetic variant and the metabolic syndrome (MetS). To the best of our knowledge, no research has yet been published concerning the Javanese population, so this study aimed to investigate the association of rs4730153 with MetS and its interaction with metabolic risk and lifestyle.ResultsThe GG genotype (p= 0.031; OR 95% CI 3.88 [1.13–13.33]), GA+GG genotype (p= 0.048; OR 95% CI 10.52 [1.02–108.01]) and G allele carrier (p= 0.006; OR 95% CI 4.19 [1.51–11.64]) of rs4730153 had a higher risk of the MetS after adjusting for obesity, hypercholesterolemia, smoking and food intake. The risk was statistically significant for the younger age group ≤ 45 years old.ConclusionThe GG, GA+GG genotype and G allele carrier of rs4730153 have a higher risk of the MetS, especially those who are obese, hypercholesterolemic and smokers and have a higher food intake in those aged ≤ 45 years old. Further larger, multicentre studies are required to confirm these pilot results.
Identified barriers and facilitators to stroke risk screening in children with sickle cell anemia: results from the DISPLACE consortium
Authors: Phillips Shannon M. , Schlenz Alyssa M. , Mueller Martina , Melvin Cathy L. , Adams Robert J. , Kanter Julie 
Published: August 2021

Abstract
Background
Children with sickle cell anemia are at risk for stroke. Ischemic stroke risk can be identified among children ages 2–16 years with sickle cell anemia using transcranial Doppler ultrasound. Despite strong recommendations for transcranial Doppler screening in guidelines released by the National Heart, Lung, and Blood Institute, implementation of transcranial Doppler screening in sickle cell anemia remains suboptimal. The purpose of this study was to identify barriers and facilitators to transcranial Doppler screening in a large national consortium to inform subsequent implementation interventions.

Methods
A qualitative descriptive approach was used to conduct 52 semi-structured interviews with a sample of patients with sickle cell anemia, their parents or primary caregivers, and healthcare providers dispersed across the United States. Interviews took place from September 2018 through March 2019. Directed content analysis was conducted with an adapted version of the Multilevel Ecological Model of Health as an initial coding framework, completed July 2019. Frequency analysis was conducted to determine predominant barrier and facilitator themes.

Results
Fourteen barrier themes and 12 facilitator themes emerged representing all levels of the ecological framework. Two barrier themes (Logistical Difficulties and Competing Life Demands and Gaps in Scheduling and Coordination), and 5 facilitator themes (Coordination, Scheduling and Reminders; Education and Information; Provider and Staff Investment and Assistance; Positive Patient Experience; and Convenient Location) were predominant.

Conclusions
Barriers and facilitators to transcranial Doppler screening in children with sickle cell anemia are complex and occur across multiple ecological levels. One barrier theme and 3 facilitator themes were found to be optimal to address in subsequent implementation interventions.
A review on corona virus and treatment approaches with Allium sativam
Authors: Pandey Rupesh Kumar , Pandey Ravindra Kumar , Shukla Shiv Shankar , Pandey Priyanka 
Published: August 2021

Abstract
Background
Recently reported cases of Covid-19 globally remind us that new diseases are coming while we are unable to provide the treatment for the same. The entire world is facing this viral attack; deaths are increasing day by day as well as infected patients too. Today, in the period of this disease, can we go to the shelter of our
traditional medicines?

Main body
In this article, we have taken medicines related to corona and conceptualized their mechanism, which gave us a chance to understand Garlic's mechanism of action, how Garlic can be a weapon in the lane with this disease. This article also tells how we can treat new diseases with our traditional herbs if no modern medicine has been discovered yet.

Conclusion
The present review is based on the structure of the virus and the targeted site for the drug discovery process with important constituents of Allium sativam. The review work also explains the allicin chemical constituent of Allium sativam which has targeted therapeutic sites related to Covid-19.
Gender Differences in Compensation in Anesthesiology in the United States: Results of a National Survey of Anesthesiologists
Authors: Hertzberg Linda B. , Miller Thomas R. , Byerly Stephanie , Rebello Elizabeth , Flood Pamela , Malinzak Elizabeth B. , Doyle Christine A. , Pease Sonya , Rock-Klotz Jennifer A. , Kraus Molly B. , Pai Sher-Lu 
Published: August 2021

BACKGROUND:
A gender-based compensation gap among physicians is well documented. Even after adjusting for age, experience, work hours, productivity, and academic rank, the gender gap remained and widened over the course of a physician’s career. This study aimed to examine if a significant gender pay gap still existed for anesthesiologists in the United States.

METHODS:
In 2018, we surveyed 28,812 physician members of the American Society of Anesthesiologists to assess the association of compensation with gender and to identify possible causes of wage disparities. Gender was the primary variable examined in the model, and compensation by gender was the primary outcome. Compensation was defined as the amount reported as direct compensation on a W-2, 1099, or K-1, plus all voluntary salary reductions (eg, 401[k], health insurance). The survey directed respondents to include salary, bonuses, incentive payments, research stipends, honoraria, and distribution of profits to employees. Respondents had the option of providing a point estimate of their compensation or selecting a range in $50,000 increments. Potential confounding variables that could affect compensation were identified based on a scoping literature review and the consensus expertise of the authors. We fitted a generalized ordinal logistic regression with 7 ranges of compensation. For the sensitivity analyses, we used linear regressions of log-transformed compensation based on respondent point estimates and imputed values.

RESULTS:
The final analytic sample consisted of 2081 observations (response rate, 7.2%). This sample represented a higher percentage of women and younger physicians compared to the demographic makeup of anesthesiologists in the United States. The adjusted odds ratio associated with gender equal to woman was an estimated 0.44 (95% confidence interval, 0.37–0.53), indicating that for a given compensation range, women had a 56% lower odds than men of being in a higher compensation range. Sensitivity analyses found the relative percentage difference in compensation for women compared to men ranged from −8.3 to −8.9. In the sensitivity analysis based on the subset of respondents (n = 1036) who provided a point estimate of compensation, the relative percentage difference (−8.3%; 95% confidence interval, −4.7 to −11.7) reflected a $32,617 lower compensation for women than men, holding other covariates at their means.

CONCLUSIONS:
Compensation for anesthesiologists showed a significant pay gap that was associated with gender even after adjusting for potential confounding factors, including age, hours worked, geographic practice region, practice type, position, and job selection criteria.
Genetic and epigenetic orchestration of Gfi1aa-Lsd1-cebpa in zebrafish neutrophil development
Authors: Wu Mei , Xu Yue , Li Jing , Lian Junwei , Chen Qi , Meng Ping , Lu Ting , Xie Huafeng , Zhang Wenqing , Xu Jin , Zhang Yiyue 
Published: August 2021

ABSTRACT
Neutrophils are the most abundant vertebrate leukocytes and they are essential to host defense. Despite extensive investigation, the molecular network controlling neutrophil differentiation remains incompletely understood. GFI1 is associated with several myeloid disorders, but its role and the role of its co-regulators in granulopoiesis and pathogenesis are far from clear. Here, we demonstrate that zebrafish gfi1aa deficiency induces excessive neutrophil progenitor proliferation, accumulation of immature neutrophils from the embryonic stage, and some phenotypes similar to myelodysplasia syndrome in adulthood. Both genetic and epigenetic analyses demonstrate that immature neutrophil accumulation in gfi1aa-deficient mutants is due to upregulation of cebpa transcription. Increased transcription was associated with Lsd1-altered H3K4 methylation of the cebpa regulatory region. Taken together, our results demonstrate that Gfi1aa, Lsd1 and cebpa form a regulatory network that controls neutrophil development, providing a disease progression-traceable model for myelodysplasia syndrome. Use of this model could provide new insights into the molecular mechanisms underlying GFI1-related myeloid disorders as well as a means by which to develop targeted therapeutic approaches for treatment.