bluuebunny/crossref_metadata_2025_split huggingface dataset:
C-type natriuretic peptide facilitates autonomic Ca2+ entry in growth plate chondrocytes for stimulating bone growth
Authors: Miyazaki Yuu , Ichimura Atsuhiko , Kitayama Ryo , Okamoto Naoki , Yasue Tomoki , Liu Feng , Ueda Yohei , Yamauchi Ichiro , Hakata Takuro , Nakao Kazumasa , Kakizawa Sho , Nishi Miyuki , Mori Yasuo , Akiyama Haruhiko , Nakao Kazuwa , Takeshima Hiroshi 
Published: September 2021

AbstractThe growth plates are cartilage tissues found at both ends of developing bones, and vital proliferation and differentiation of growth plate chondrocytes are primarily responsible for bone growth. C-type natriuretic peptide (CNP) stimulates bone growth by activating natriuretic peptide receptor 2 (NPR2) which is equipped with guanylate cyclase on the cytoplasmic side, but its signaling pathway is unclear in growth plate chondrocytes. We previously reported that transient receptor potential melastatin-like 7 (TRPM7) channels mediate intermissive Ca2+ influx in growth plate chondrocytes, leading to activation of Ca2+/calmodulin-dependent protein kinase II (CaMKII) for promoting bone growth. In this report, we provide experimental evidence indicating a functional link between CNP and TRPM7 channels. Our pharmacological data suggest that CNP-evoked NPR2 activation elevates cellular cGMP content and stimulates big-conductance Ca2+-dependent K+ (BK) channels as a substrate for cGMP-dependent protein kinase (PKG). BK channel-induced hyperpolarization likely enhances the driving force of TRPM7-mediated Ca2+ entry and seems to accordingly activate CaMKII. Indeed, ex vivo organ culture analysis indicates that CNP-facilitated bone growth is abolished by chondrocyte-specific Trpm7 gene ablation. The defined CNP signaling pathway, the NPR2-PKG-BK channel-TRPM7 channel-CaMKII axis, likely pinpoints promising target proteins for developing new therapeutic treatments for divergent growth disorders.
The nuts and bolts of SARS-CoV-2 Spike Receptor Binding Domain heterologous expression
Authors: Maffei Mariano , Montemiglio Linda Celeste , Vitagliano Grazia , Fedele Luigi , Sellathurai Shaila , Bucci Federica , Compagnone Mirco , Chiarini Valerio , Exertier Cécile , Muzi Alessia , Roscilli Giuseppe , Vallone Beatrice , Marra Emanuele 
Published: September 2021

AbstractCOVID-19 is a highly infectious disease caused by a newly emerged coronavirus (SARS-CoV-2) that has rapidly progressed into a pandemic. This unprecedent emergency has stressed the significance of developing effective therapeutics to fight current and future outbreaks. The receptor-binding domain (RBD) of the SARS-CoV-2 surface Spike protein is the main target for vaccines and represents a helpful “tool” to produce neutralizing antibodies or diagnostic kits. In this work, we provide a detailed characterization of the native RBD produced in three major model systems: Escherichia coli, insect and HEK-293 cells. Circular dichroism, gel filtration chromatography and thermal denaturation experiments indicated that recombinant SARS-CoV-2 RBD proteins are stable and correctly folded. In addition, their functionality and receptor-binding ability were further evaluated through ELISA, flow cytometry assays and bio-layer interferometry.
Perception and adaptation of agricultural households to climate change in the semi-arid regions of Rajasthan – a gender perspective study
Authors: Mohapatra Geetilaxmi , George Meera 
Published: September 2021

PurposeThe study aims to analyze the gender-wise perception of the agricultural households toward climate change and the adaption measures taken by these households, especially women, to mitigate climate changes.Design/methodology/approachPurposive random sampling technique is used to collect primary data from a pilot survey conducted in two semi-arid districts of Rajasthan, India. Data mainly focused on analyzing the gender-based perception and adaptation strategies undertaken toward climate change. And descriptive statistics are used for analysis.FindingsThe study found that both the gender are aware of the climatic changes. Deforestation increased population, change in living standards, urbanization and industrialization contribute to climate changes. The women are employing limited adaptation strategies to mitigate the climatic stress compared to males.Research limitations/implicationsThis is a pilot study; hence, it has an insufficient sample size for the detailed statistical analysis. Further, it is only limited to two semi-arid districts of Rajasthan.Originality/valueThis pioneering study highlights gender-wise differences in perception and adaptation strategies undertaken in this region. The study suggests raising awareness about climate change and providing credit facilities for undertaking adaptation measures to reduce agricultural households' vulnerability, particularly enhancing women's adaptive capacity to climate change.
Future of work: a systematic literature review and evolution of themes
Authors: Singh Anjali , Jha Sumi , Srivastava Dinesh Kumar , Somarajan Abheesh 
Published: September 2021

Purpose
Concerned with the rising social, economic and technological disruption in the world, the impact of the technological disruption had a significant impact on the future of work and it has been tremendously increased in past five years. Further, with the rising uncertainties and COVID-19 in the picture, the trends suggested by earlier literature might not hold. The purpose of this paper is to understand the evolution of technology in the workplace in the past five years, how does it stand during COVID-19, will the trend continue in light of disruption caused by COVID-19, the impact of COVID-19 on the future of work.

Design/methodology/approach
The paper uses bibliometric techniques to identify the conceptual and intellectual structure of the studies. The programming language named after authors Robert and Ross (R) software and Biblioshiny were used to identify the structures and the themes underlying those structures, which further helped in forecasting the trends of the studies.

Findings
The paper shows the drastic evolution of the studies in past few years and different technologies implemented at the workplace in the same period. It further identified the influential papers, authors, journals in the area with an emphasis on the various collaboration network among authors and countries. It also paints a picture of the impact of COVID-19 on the future of work. The paper finally concluded with future directions for the emerging trends and themes in the area in aftermath of COVID-19.

Originality/value
This paper takes the microscopic view of the studies carried out in the past five years as during the past five years, the studies related to this topic have grown tremendously and accordingly many trends have been identified but with the COVID-19 pandemic in the picture, the trend is likely to get accelerated. This paper takes this view and identifies the trends in the future by identifying the themes based on periods and at different levels – organizational, managerial, individual.
Analysis of global economic policy uncertainty and export flows for emerging markets with panel VAR modeling
Authors: Aslan Çağlayan , Acikgoz Senay 
Published: September 2021

PurposeThe purpose of this paper to examine how global economic policy uncertainty (GEPU) affects export flows of emerging market economies.Design/methodology/approachThis study examines the effect of GEPU on 28 emerging markets' export performance. GEPU variable used in the authors’ empirical analysis is measured by partial least square (PLS) factor loading model with the help of 24 countries' economic policy uncertainty index. A panel vector autoregression (VAR) model is employed for the estimations and monthly data over the 2006:01–2019:12 period are used.FindingsThe empirical findings show that while the real external income is the main factor that affects export flows, the real exchange rate is the least effective variable with regard to the variance decomposition, which is not expected by the related economic theory. Panel VAR estimations results confirm the previous studies and find that GEPU affects export flows negatively and significantly.Originality/valueTo the best of the authors’ knowledge, this is the sole study in terms of focusing on the impacts of GEPU on the export volume of emerging markets. The contribution of this paper is twofold. Firstly, a large set of countries with monthly frequented data that assist to capture uncertainties better is used. Secondly, the global economic policy index is obtained by employing the PLS method, which provides more robust results that are calculated with respect to the dependent variable.
The symbolic value of wine, moderating and mediating factors and their relationship to consumer purchase intention
Authors: De Toni Deonir , Pompermayer Rogério , Lazzari Fernanda , Milan Gabriel Sperandio 
Published: September 2021

PurposeThe symbolic value of wine is a relevant research topic and raises the interest in studies in both the enological and market areas. In this context, this study aims to understand the role of the symbolic value of wine and its relationship to the product purchase intention.Design/methodology/approachThe study is based on a survey of 269 wine consumers from Brazil. The basic theoretical framework includes three latent constructs (symbolic value, consumer attitude and product-norm experience) and three moderators (consumer involvement, willingness to pay and consumer preference). Relations between these are analyzed using the confirmatory factor analysis (CFA) and the moderated mediation analyses using Haye’s process.FindingsThis research identified that the symbolic value is totally mediated by consumers’ attitudes and product-norm experiences. However, such a relationship occurs directly for consumers with higher involvement with the product, higher willingness to pay, and who assume that wine is their preferred alcoholic beverage.Originality/valueOne of the contributions is to emphasize the symbolic value of wine and highlight how the relationship with different factors can interfere and explain consumer purchase intention and can influence the strategies, actions and investments of companies in the sector.
Relocation of public healthcare network of a large city in the event of a major earthquake: a combined methodological analysis
Authors: Garrido Alexander , Pongutá Fabián , Buitrago Oscar Yecid 
Published: September 2021

PurposeThe aim of this research is to improve the responsiveness of the healthcare network of a large city to a major earthquake, by applying a combined methodology to reduce human suffering and death.Design/methodology/approachScenario analysis, a non-linear programming (NLP) model, and the analytical network process are sequentially applied to find the “best location pattern”.FindingsWhen considering the occurrence of major earthquakes in cities with high population density, as a rule of thumb, the location of healthcare facilities should prioritize areas characteristically overcrowded and/or that were built based on poor standards of seismic resistance.Research limitations/implicationsThe proposed research design does not include a cost criterion in the set of decision variables involved. Furthermore, the results derived from the NLP-model are restricted by the input simulation data.Practical implicationsThe performance of the “best location pattern” is compared with the current location of healthcare facilities in terms of their distances to the affected zones. Metropolis areas worldwide with similar conditions to the city under consideration could be benefited from applying the general methodology for relocation of healthcare facilities described in this research.Originality/valueThis research implements a diverse combination of methodologies to examine the problem of relocating of healthcare facilities in a large city in the wake of an assumed earthquake. In addition, to the best of authors' knowledge, this is the first study of its kind that proposes improvements in the responsiveness of the healthcare facilities' network in the city in question.
The neglected contributions of self-efficacy to older adults’ financial capacity
Authors: Waheed Hassam 
Published: September 2021

Purpose
An ageing population comes with its own set of challenges such as impaired financial capacity and resultant dependency on others to manage financial affairs. Dependency, in turn, as the evidence suggests, creates opportunities for financial exploitation of older adults. Related studies have primarily examined the clinical features and correlates of financial capacity or have attempted to develop its multidimensional measures. Both of which do little to resolve issues associated with impaired financial capacity. This paper aims to make a case for future researchers to assess older adults’ financial capacity from a non-clinical aspect.

Design/methodology/approach
Drawing on the notion of self-efficacy, as encapsulated within the social cognitive theory, this paper presents evidence from a host of different domains to demonstrate the potential contributions of self-efficacy to older adults’ financial capacity.

Findings
The contributions of self-efficacy in preserving older adults’ financial capacity appear to be much more profound than is currently acknowledged in the literature, thereby overlooking potentially promising and cost-effective interventions for autonomous ageing.

Originality/value
This paper presents a novel application of self-efficacy to autonomous ageing. Within this context, potential routes to the deployment of self-efficacy-based interventions are also discussed.
Does the quality of a youth academy impact a football player's market value?
Authors: Balliauw Matteo , Bosmans Jasper , Pauwels David 
Published: September 2021

PurposeFootball clubs invest in the implementation of scientific insights that improve the quality of youth academies. In the long run, clubs expect their youth academy investments to result in better trained players. The purpose of this paper is to quantify the impact of the attended youth academies' quality on the future market value of a player.Design/methodology/approachA dataset containing 94 players trained in 13 different academies has been constructed. The dataset contains characteristics of the players and information on the quality of their attended academies. The impact of the quality of the attended academies on players' future market values was estimated empirically through multiple regression analysis.FindingsThe quality of a youth academy has a significant positive impact on a player's market value, which in turn is correlated with higher future wages for players and transfer fees for clubs.Research limitations/implicationsClubs are advised to pay sufficient attention to investments in their youth academy. This will eventually lead to better trained players and higher revenues. Players in turn should strive to be part of the best academies that provide good training and the opportunity to become a top-earning player. For policymakers, such as football federations, the results imply that stimulating club investments in academies can lead to better national team performances.Originality/valueThe impact of the quality of a youth academy on an individual professional football player's career has never been quantified in the literature before. To this end, a new variable has been constructed using scientific assessments of youth academies.
The past is a feline country? Cuteification-Value Nexus and World Heritage visitor engagement
Authors: Ong Chin Ee 
Published: September 2021

Purpose
This paper responds to the lack of visitor engagement in many culture-based World Heritage sites and conceptualises a “Cuteification-Value Nexus” for the discussion of the communication of heritage values through “cute” or aesthetically pleasing popular culture elements. It reflects on observations in Macao to argue for a greater engagement of culture-based World Heritage sites through a combination of popular culture inspired motifs and truthful heritage messages. Specifically, it identifies a form of “cuteified heritage” – a hyperreal cultural zone that happens away from the actual heritage sites, but which articulates the heritage significances of those sites. This draws on concepts on themed spaces and insights from postmodernistic hyperreality and tourism to examine how the “completely real” becomes identified with the “completely fake” in the staging, consumption and negotiation of experiences with World Heritage and their utility in the management of World Heritage tourism sites.
Detection of the serological markers hepatitis B virus surface antigen (HBsAg) and hepatitis B core IgM antibody (anti‐HBcIgM) in the diagnosis of acute hepatitis B virus infection after recent exposure
Authors: de Almeida Pondé Robério Amorim 
Published: September 2021

AbstractThe serological diagnosis of acute hepatitis B virus (HBV) infection after recent exposure has been established by the hepatitis B virus surface antigen (HBsAg) and anti‐hepatitis B core IgM antibody (anti‐HBcIgM) detection in serum, sometimes accompanied by the detection of hepatitis B “e” antigen (HBeAg). Despite this characteristic serological profile, misdiagnosis can occur in cases of unexpected or atypical behavior of the serological markers in the bloodstream, or if the true meaning of its expression is not properly investigated, or even if there is a possibility of interference from factors not necessarily linked to the infectious process, in the detection of these markers. This review discusses the influence of these variables on laboratory results for these two serological markers and, therefore, the potential risk of these variables compromising the correct diagnosis of acute infection after recent HBV exposure.
Diagnostic testing in nontrauma patients presenting to the emergency department with recurrent seizures: A systematic review
Authors: Burgess Michael , Mitchell Robert , Mitra Biswadev 
Published: September 2021

AbstractBackgroundThere is a lack of consensus regarding the role of investigations among patients presenting to the emergency department (ED) with recurrent seizures. The aim of this systematic review was to determine the frequency and utility of commonly requested investigations for nontrauma patients presenting to the ED with recurrent seizures.MethodsThe MEDLINE, EMBASE, and Cochrane Library databases were searched (March 2021) for articles on this topic using search terms related to recurrent seizures, investigations, and the ED. The inclusion criteria required that articles include adult nontrauma patients presenting to the ED. Studies exclusively investigating first‐episode seizures, trauma patients, and status epilepticus were excluded. Eligible studies were assessed for bias using the Newcastle‐Ottawa scale. Results of studies were presented using proportions.ResultsThere were six cohort studies included that contributed data from 36,595 patients. All six studies assessed at least one of our primary outcomes for computed tomography (CT) brain scans. The proportion of patients who underwent a head CT ranged from 13% to 42%. The rates of abnormal head CT findings ranged from 8% to 21%. One study reported on magnetic resonance imaging (MRI) and found it used infrequently in 0.79% of cases. The proportion and yield of nonneuroimaging investigations were not well evaluated in this patient population. Only one study reported on the utility of sodium levels or blood glucose results for this population and reported abnormalities in sodium levels for 19% of patients and abnormalities in glucose levels in 50% of patients.ConclusionsIn this population, CT brain scans appeared to be performed uncommonly but with moderate rates of abnormal findings. In the absence of prolonged alteration of consciousness, a history of brain tumor, or positive neurologic findings, however, neuroimaging was of low yield. Given the heterogeneity and potential limitations of these studies, further research on this topic is required.
Agricultural development in industrialising Japan, 1880–1940
Authors: Arimoto Yutaka , Sakane Yoshihiro 
Published: September 2021

AbstractThis study reviews research examining agricultural development in industrialising Japan. We focus on the (dys)functioning of markets for land, finance, labour and agricultural commodities. We cover topics including land (mis)allocation, size‐productivity relationships, tenancy contract choice and Marshallian inefficiency, property rights, microfinance, shock‐coping strategies, rural–urban migration and agricultural market integration. The literature reveals that market failures often observed in developing economies were not prominent, except for possibly labour markets. The literature also highlights the roles and administrative capacities of central and local governments. Tight local communities served to reduce transaction costs.
Long‐term risk of upper gastrointestinal bleeding after Helicobacter pylori eradication: a population‐based cohort study
Authors: Jiang Fang , Guo Chuan‐Guo , Cheung Ka Shing , Leung Wai K. 
Published: September 2021

SummaryBackgroundThe long‐term effects of H. pylori eradication in preventing upper GI bleeding (UGIB) remains unknown.AimTo determine the long‐term risks of UGIB after H. pylori eradicationMethodsWe included all patients who had received clarithromycin‐containing triple therapy for the treatment of H. pyliori infection between 2003 and 2012, without subsequent need for re‐treatment. We included a propensity score (PS)‐matched endoscopy cohort of H. pylori‐negative patients as controls. The primary endpoint was the risk of subsequent UGIB. A multivariable Cox model was used to compute the hazard ratio (HR) of UGIB.ResultsWe included 62 738 H. pylori‐eradicated and 62 738 PS‐matched H. pylori‐negative patients, with a median follow‐up of 8.1 years (IQR 5.5‐10.6). The incidence of UGIB was 20.8 (95% CI 19.5‐22.1) and 13.6 (95% CI 12.7‐14.7) per 10 000 person‐years in H. pylori‐eradicated and H. pylori‐negative patients, respectively. Compared to controls, H. pylori‐eradicated patients had a significantly higher risk of UGIB (HR: 1.65, 95% CI 1.49‐1.83). The risk of UGIB in H. pylori‐eradicated patients increased after the first 2 years of follow up (HR: 2.18, 95% CI 1.91‐2.49). Age‐stratified analysis showed that patients >45 years had higher UGIB risk, even after eradication.ConclusionsDespite H. pylori eradication, the long‐term risk of UGIB was still higher than in H. pylori‐negative control subjects. The protective effects of eradication therapy in preventing UGIB appeared to be limited to younger patients, and to within the first 2 years after eradication.