bluuebunny/crossref_metadata_2025_split huggingface dataset:
PSV-B-17 Additional dietary choline increases serum metabolites as determined by direct infusion mass spectrometry in post-gonadectomy kittens
Authors: Godfrey Hannah , Rankovic Alexandra , Grant Caitlin E , Shoveller Anna-Kate , Abood Sarah , Bakovic Marica , Verbrugghe Adronie 
Published: October 2021

Abstract
This study aimed to investigate changes in serum metabolites using direct infusion mass spectrometry (Di-MS) after 12 weeks with or without additional choline in growing, post-gonadectomy kittens. The present study was approved by the University of Guelph Animal Care Committee (AUP#4118). Intact, male kittens (3-months old; n = 15) were fed a base-diet to growth requirements (3310mg choline/kg DM) over an 11-week acclimation. Kittens were gonadectomized (week 1) and assigned to base-diet (n = 7) or base-diet with 300mg/kgBW0.75 additional choline (n = 8) and fed to mimic ad libitum food intake (3 meals totaling thrice daily requirements) for 12-weeks. Fasted serum samples were obtained and analyzed for 130 metabolites via Di-MS (week 0 and 12). The proc GLIMMIX procedure (SAS) was used to analyze differences between groups as a repeated measure. Additional choline resulted in higher serum concentrations of one-carbon metabolites such as methionine, serine, betaine, and sarcosine (P &lt; 0.05). As expected, serum concentrations of phosphatidylcholines increased with additional choline, as did serum concentrations of lysophosphatidylcholines and sphingomyelins, compared to no additional choline (P &lt; 0.05). There was minimal impact on acyl carnitines with a decrease in medium length acyl carnitines (P &lt; 0.05). Additionally, there was an increase in serum concentrations of spermine, trans hydroxyproline, methionine sulfoxide, proline, and indole acetic acid, and a decrease in serum trimethylamine N-Oxide compared to control (P &lt; 0.05). Effects of growth stage or gonadectomy may have resulted in increased serum amino acid concentrations such as asparagine, glutamine, glutamic acid, histidine, lysine, methionine, threonine, and tyrosine in both groups (P &lt; 0.05). These results may indicate benefits for providing additional choline to post-gonadectomy kittens on one-carbon metabolism and fatty acid oxidation which could promote healthy weight and body condition. However, more research is warranted to further understand choline’s role in energy balance and its potential in prevention and treatment of feline obesity.
PSIII-23 Maternal metabolizable protein level and rumen-protected methionine impacts on beef steer progeny gene expression in muscle and liver
Authors: Acton Kortney , Mandell Ira B , Huber Lee-Anne , Steele Michael A , Wood Katharine M 
Published: October 2021

Abstract
This experiment evaluated how maternal metabolizable protein level and rumen-protected methionine supplementation during late gestation impacts expression of genes associated with fetal programming in beef steer offspring. In a 3 x 2 factorial arrangement, 138 Angus crossbred cows were randomly assigned one of three metabolizable protein (MP) treatments (fed to meet 90, 100, or 110% of MP requirements), with(without) 9 g/d of rumen-protected methionine (MET; Smartamine®M, Adisseo Inc.). The isocaloric diets were fed for the last 8 weeks of gestation. Steer progeny (n = 51) were assigned to one of seven pens by weight and received a common corn-based grower diet (58% corn silage, 26% alfalfa haylage, 15% soybean meal) for 47 days, followed by a finisher diet (78% high moisture corn, 12% alfalfa haylage, 8% soybean meal) for 115 ±31.5 days until slaughter. At slaughter, samples of Sternomandibularis muscle and liver were collected and snap-frozen in liquid nitrogen for RNA isolation and analysis of gene expression for growth, myogenic, and adipogenic genes. Data were analyzed using PROC GLIMMIX in SAS, with maternal MP level and MET supplementation as fixed effects, and pen as a random effect. Exceeding maternal MP requirements increased expression of myogenic regulatory factor, myogenin (MYOG; 90% MP: 0.72, 100% MP: 0.94, and 110% MP: 1.00; SEM = 0.083, respectively; P = 0.02) in muscle from steer progeny at slaughter. Methionine supplementation decreased expression of pyruvate kinase (PKM; MET: 0.81, No MET: 1.16; SEM = 0.092; P = 0.02) in muscle, which is associated with lean tissue growth. Maternal nutritional treatment did not influence hepatic gene expression (P ≥ 0.06). These data suggest that maternal nutrition may impact progeny muscle development, while maintaining metabolic function of the liver.
PSXI-13 Effects of pasture grazing on growth performance and blood composition of Hanwoo steer during the growing stage
Authors: Jung Jeongsung , Yang Seung Hak , Lee Baehun , Choi Ki Choon 
Published: October 2021

Abstract
This study investigated the effect of pasture grazing on growth performance and blood characteristics in nine-month-old Hanwoo (native Korean cattle) steers. Thirty one steers were randomly assigned to three groups: (CON) Italian ryegrass and concentrate (1.6% BW) until 14 month of age, (G1) pasture grazing 101 days without concentrate, (G2) pasture grazing 101 days and concentrate (1.6% BW). Initial BW of CON, G1 and G2 were 255 kg, 238 kg and 254kg respectively. Average daily gains were higher in CON (1.02 kg) and G2 (0.92 kg) than G1 (0.62 kg) (p &gt;0.05). Final weight and total weight gain were higher in the feeding concentrate (1.6% BW) groups (Con and G2) compared to the G1. Blood characteristics, such as such as total cholesterol (T-CHO), triglycerides (TG) and non-esterified fatty acid (NEFA) were investigated to test the effects on health and energy metabolism. T-CHO, an index of fat metabolism and liver function, was lower in Con (119.75 md/dL) and G2 (107.13md/dL) than G1 (87.79 mg/dL). TG was higher in Con (21.63 IU/L) than G1(15.14 IU/L) (P&lt; 0.08). NEFA was higher in G2 (384 uEq/L) than other groups (Con 138 uEq/L and G1 225 uEq/L). These results show that concentrates supplementation during grazing period is needed to increase BW of Hanwoo steer in the growing state.
PSXI-15 Evaluation of low-lignin alfalfa ‘Hi-Gest® 360’ on the Canadian prairies: productivity, nutrient profile, and rumen degradation kinetics
Authors: Damiran Daalkhaijav , Biligetu Bill , Pearce Leah , Lardner Herbert 
Published: October 2021

Abstract
The study was conducted at Saskatoon, SK, Canada in the Dark Brown soil zone (52°07′N,106°38′W), to compare low-lignin Hi-Gest®360 alfalfa (Hi-Gest; Medicago sativa L.) to a conventional alfalfa (AC Grazeland) under two cutting regimes. Each cultivar was seeded in 2017 as both a monoculture and binary mixture (binary; Hi-Gest+HB and Grazeland+HB) with AC Success hybrid bromegrass (HB). Plot size was 1.2m×6m with 4 rows. Three years (2018–2020) result suggested that the first cut DMY was 1695±241 and 2151±276 kg/ha for the Hi-Gest and Grazeland, respectively. Hi-Gest had greater (P &lt; 0.05) TDN (68.4 vs. 66.6%, DM basis), NEm (1.7 vs. 1.6 Mcal/kg), IVNDFD48: (42.9 vs. 37.8%), but lower second cut DMY (1288 vs. 1560 kg/ha), ADF (26.3 vs. 28.7%), NDF (34.7 vs. 36.6%), compared to AC Grazeland. Under two cutting, Hi-Gest was numerically lower in total DMY (2983 vs. 3712 kg/ha; P = 0.114) relative to Grazeland. In first cut forage, Hi-Gest also was numerically lower in ADL (5.9 vs. 6.5%; P = 0.57) relative to AC Grazeland. Hi-Gest was similar (P = 0.15) with AC Grazeland in effectively degradable dry matter (EDDM; 622 g/kg DM), effectively degradable CP (EDCP; 689 g/kg CP). Hi-Gest alfalfa had greater (P = 0.05) effectively degradable neutral detergent fiber (EDNDF; 19% more; 340 vs. 285 g/kg NDF) relative to AC Grazeland alfalfa. In binary system, Hi-Gest+HB had (P = 0.02) greater EDCP (12% more; 526 vs. 468 g/kg CP), and numerically greater EDNDF (4.4% more; 259 vs. 248 g/kg NDF; P = 0.10) but had similar EDDM (409 g/kg DM) relative to Grazeland+HB mixture. Each percentage unit increase in alfalfa ADL concentration decreased EDNDF by 2.3 percentage units (EDNDF, % NDF = 46.0–2.93×ADL, % DM, r2=0.26, P &lt; 0.01). Although Hi-Gest yielded less (~25% less) than AC Grazeland, Hi-Gest had greater nutritive value compared to AC Grazeland; differed little for yield and quality in binary mixture, suggesting Hi-Gest can be a viable alternative legume for western Canadian prairies.
PSII-B-25 Age-related changes in growth hormone-insulin like growth factor 1 axis-regulating factors and metabolic hormones in Korean cattle steer
Authors: Lee Jinoh , Beak Seok-Hyeon , Kim Do Hyun , Lee Jae Sung , Cho In Gu , Baik Myunggi 
Published: October 2021

Abstract
Meat yield is important for beef industry for maximum profits. Enhancing muscle growth by promoting hypertrophy of muscle fiber is required for increasing meat yield. Growth hormone (GH)-insulin like growth factor 1 (IGF1) axis and metabolic hormones have a central role for regulating hypertrophy of muscle fiber. We investigated age-related changes in GH-IGF1 axis-regulating factors and metabolic hormones in beef cattle. Ten Korean cattle steers were used. The longissimus thoracis (LT) samples were biopsied between the 11th and 12th rib at 12, 18, and 24 months of age. Steers were slaughtered at 34 month of age and the LT samples were collected. The LT samples were used for histological observation of muscle fiber size and detection of mRNA levels by real-time PCR analysis. Blood was collected at 12, 18, 24, and 34 months of age and used for hormone analyses. The mean muscle fiber size increased (P &lt; 0.01) from 12 to 24 months, and remained unchanged at 34 months. Plasma GH concentrations were unchanged with age, whereas circulating IGF-1 concentrations decreased (P &lt; 0.001) with age. Testosterone and insulin concentrations increased from 12 to 24 months and remained unchanged at 34 months. Leptin concentrations were unchanged from 12 to 24 months and increased at 34 months. The mRNA levels of growth hormone receptor (GHR) and signal transducer and activator of transcription 5a/b (STAT5a/b) decreased (P &lt; 0.01) from 12 to 24 months, whereas mRNA levels of IGF-1 gene increased (P &lt; 0.001) with age. Results indicate that the increased muscle fiber size in LT from 12 to 24 months are characterized with increasing concentrations of circulating testosterone and insulin. Age-dependent changes in muscle fiber hypertrophy were also related with changes in GHR, STAT5a/b, IGF-1 mRNA levels in the LT.
PSII-B-26 Comparison of Suffolk and Texel sired lamb growth and satellite cell proliferation in the early postnatal period
Authors: Greene Maslyn A , Duckett Susan K 
Published: October 2021

Abstract
Suffolk ewes (n = 26) were blocked by body condition and weight, and randomly divided into two breeding groups (n = 13/group). One group was bred to a Texel ram and the other to a Suffolk ram. Ewes were confirmed pregnant by ultrasound and went to term. Male lambs (n = 4/sire/time) were harvested on d 2, 14, and 203 of age for muscle characterization and satellite cell isolation. From d 2 to 14, body weight increased (P &lt; 0.01) by 80%, weight of the longissimus increased by 160%, and longissimus muscle area increased by 100%. Between d 14 and 203 lamb weight and weight of the longissimus increased by 350%, while longissimus area only increased by 100%. Sire breed did not alter lamb weight or weight of the longissimus (P &gt; 0.10). Longissimus muscle area tended (P &lt; 0.10) to be increased for Texel sired lambs when compared to Suffolk sired lambs. The total number of satellite cells isolated were not different by sire breed but did differ by animal age (P &lt; 0.01). Satellite cell populations per gram of tissue increased between d 2 and 14 and decreased from d 14 to 203. Due to the large changes in growth from d2 to 14, satellite cell proliferation was examined at d 2 of age. Satellite cell proliferation capacity was altered by sire breed at d 2 of age potentially contributing to the increased longissimus muscle area. Advancing animal age and development alters satellite cell population numbers and potentially supports a high capacity for growth.
PSII-B-28 Investigation of the presence of bacterial microbiota in 12-week-old bovine fetuses
Authors: Amat Samat , Holman Devin B , Schmidt Kaycie , McCarthy kacie L L , Dorsam Sheri T T , Ward Alison K K , Borowicz Pawel P P , Reynolds Lawrence P P , Caton Joel , Sedivec Kevin K K , Dahlen Carl R 
Published: October 2021

Abstract
A recent study reported the existence of a diverse microbiota in 5-to-7-month-old calf fetuses, suggesting that colonization of the bovine gut with so-called “pioneer” microbiota may begin during mid-gestation. In the present study, we investigated the microbiota in bovine fetuses at early gestation. Amniotic and allantoic fluids, and intestinal and placental (cotyledon) tissue samples harvested from fetuses (n = 33) on day 83 of gestation were processed for the assessment of fetal microbiota using 16S rRNA gene sequencing. The sequencing results revealed that a diverse and complex microbial community was present in allantoic and amniotic fluids, and fetal intestine and placenta on day 83 of gestation in beef cattle. Microbial community structure was significantly different between allantoic and amniotic fluid, and intestinal and placental microbiota (0.047 ≥ R2 ≥ 0.019, P ≤ 0.031). Allantoic fluid had a greater (P &lt; 0.05) microbial richness (number of OTUs) (122 ± 10) compared to amniotic fluid (84 ± 6), intestine (63 ± 7) and placenta (66 ± 6). Microbial diversity (Shannon index) was similar for the intestinal and placental samples, and both were less diverse compared to the fetal fluid microbiota (P &lt; 0.05). At the phylum level, 39 different archaeal and bacterial phyla were detected across all fetal samples, with Proteobacteria (55%), Firmicutes (16.2%), Actinobacteria (13.6%) and Bacteroidetes (5%) predominating. Among the 20 most relatively abundant bacterial genera, Acidovorax, Acinetobacter, Brucella, Corynebacterium, Enterococcus, Exiguobacterium and Stenotrophomonas differed by fetal sample type (P &lt; 0.05). A total of 55 taxa were shared among the four different microbial communities. qPCR of bacteria in the intestine and placenta samples as well as scanning electron microscopy imaging of fetal fluids provided additional evidence for the presence of a microbiota in these samples. Overall, the results of this study indicate that colonization with pioneer microbiota may occur during early gestation in bovine fetuses.
PSVIII-26 Utilizing Megasphaera elsdenii probiotic to improve the performance of crossbred Holstein x Angus calves
Authors: Mazon Gustavo , Costa Joao H C 
Published: October 2021

Abstract
This study evaluated the effect of an oral probiotic bolus (Megasphaera elsdenii NCIMB 41125; Lactipro Advance®, MS Biotec, Wamego, KS) on the performance of crossbred Holstein x Angus calves. Thirty-one calves (45.3 ± 7.1 kg; 8.2 ± 2.0 d old) were enrolled in a 76-day randomized block trial in two blocks. This study was conducted at the University of Kentucky Large Animal Unit between August 2020 and April 2021. Calves were assigned to one of three probiotic treatments: no probiotic (ME0), single dose of probiotic on day 15 (ME15), or probiotic administration on day 15 and 39 (ME15 + 39). Calves were individually housed and had ad libitum access to water and a pelletized calf starter (18% CP, 2.5% fat). Calves were fed 7 L/d of milk replacer (Cows Match Warm Front, Land O Lakes, MN; 1L, 150 g/L) divided into two equal meals until day 41. On day 42, milk allowance was reduced in half and then weaned on day 56. Grain orts were weighed daily and grain intake was calculated for each calf. Individual body weights were measured weekly. A mixed linear model determined the effect of treatment on total grain dry matter intake (total DMI), total weight gain (TWG), average daily gain (ADG), and feed conversion ratio (FCR). Manual stepwise backward elimination retained predictors with a P-value &lt; 0.30 of the factors block, treatment, age and weight at enrollment, and if treated with antibiotics. Calves receiving the M. elsdenii bolus had greater TWG (ME0=53.57±5.83, ME15=72.52±6.71, ME15 + 39=77.51±5.00 kg; P = 0.01), greater ADG (ME0=0.73±0.08, ME15=0.99±0.09, ME15 + 39=1.05±0.07 kg/d; P = 0.01), and tended to have greater total DMI (ME0=72.24±10.49, ME15=95.20±12.07, ME15 + 39=105.36±9.01 kg; P = 0.06) throughout the study. FCR did not differ between treatments (ME0=0.79±0.04, ME15=0.78±0.04, ME15 + 39=0.76±0.03 kg; P = 0.84). Overall, strategic pre-weaning intervention of a M. elsdenii bolus improved the performance of crossbred Holstein x Angus calves.
PSVII-16 Effects of different forms of Cu on in vitro ruminal fermentation and digestibility of a lactation diet
Authors: Boyer Angela R , Jiang Yun , Blakeney Alon , Nuzback Dennis , Humphrey Brooke , Garcia Miriam 
Published: October 2021

Abstract
Vistore® mineral products are hydroxychloride minerals that feature high metal content and improved bioavailability. This study was conducted to compare different sources of copper (Cu) on in vitro rumen fermentation parameters. Three ruminally-cannulated Jersey heifers were adapted to a lactation diet for two weeks before being used as donors. Three sources of Cu at 4 ppm: No supplemental Cu (CON), CuSO4, Vistore Cu, and another Cy hydroxychloride product (Vistore-competitor). The concentration of Cu in this study was selected from a titration study (0 to 8 ppm CuSO4) to identify the minimum concentration of CuSO4 affecting rumen fermentation. The lactation diet (TMR) was dried and ground to 1mm and used as the substrate. Rumen fluid was collected two hours after feeding. Substrate (0.5 g) was inoculated with 100 mL of a 3:1 McDougall’s buffer: ruminal fluid mixture at 39ºC for 24 h. Each treatment was run in triplicate and in three runs. Data were analyzed with R 4.0. The model included fixed effect of treatment and random effect of run. CuSO4 tended to increase lag time (0.78 vs -0.57 h, P = 0.06), reduced (P &lt; 0.05) DMD (52.4 vs. 56.1%), cellulose digestibility (4.9% vs. 41.9%), isobutyrate molar % (0.58 vs. 0.78%) and NH3-N concentration (5.46 vs. 6.91 mg/dL). Vistore and Vistore-competitor maintained the fermentation and digestibility compared to CON. In general, Vistore Cu and Vistore-competitor maintained ruminal fermentation and digestibility parameters while negative effects of CuSO4 were observed. These results indicate different Cu mineral sources may affect the rumen differently.
PSXIII-7 Effects of different organic pastures on dairy heifer rumen fluid characteristics
Authors: Briggs Reganne K , Hadfield Jacob , Rose Marcus , Stott Rusty , Waldron Blair , Young Allen Y , Isom Clay , Rood Kerry , Thornton Kara J 
Published: October 2021

Abstract
This study investigates the effects that different forages in a rotational grazing system have on pH and VFA proportions of rumen fluid in organically raised dairy heifers. In 2018, 81 yearling Jersey heifers were assigned to one of nine treatments, including a conventional dry lot control (TMR) or one of eight pasture treatments. Pasture treatments included: tall fescue (TF), meadow bromegrass (MB), orchard grass (OG), perennial ryegrass (PR) and each individual grass interseeded with birdsfoot trefoil (BFT). Every 35-d, over a 105-d period, rumen fluid was collected using an oral stomach tube. Immediately following collection, pH was measured and recorded. Rumen fluid collected for VFA analysis was stabilized with acid and placed on wet ice and eventually frozen at -80° C until further analysis. Repeated measures analyses were performed using PROC MIXED in SAS (version 9.4) to determine how treatment affected each measurement over the total 105-day period. Time had a significant effect (P &lt; 0.001) on pH ofrumen fluid over the 105-d growth period. Additionally, treatment had a significant effect (P &lt; 0.001) on pH of rumen over the 105-d growth period. Animals that consumed the TMR diet had decreased rumen pH compared to other treatments. The proportions of acetate, propionate, isobutyrate, butyrate, isovalerate, and valerate were all significantly affected (P &lt; 0.001) by time over the 105-d growth period. Treatment had an effect (P &lt; 0.05) on the proportions ofpropionate and butyrate and had a tendency (P = 0.06) to affect the proportion of isovalerate. In general, animals on the TF pastures had decreased (P &lt; 0.05) proportions of these VFAs in the rumen fluid. The proportions of acetate, isobutyrate, and valerate were not affected (P &lt; 0.05) by treatment. These data provide insight into how different forages in organic pastures affect rumen characteristics of growing Jersey heifers.
PSXIV-9 Predicting dry matter intake in beef cattle with water intake and other variables
Authors: Wilson Matthew E , Shaffer Kevin , Plum Shane , Garossino Kevin , Lansink Nicky , Hubbart Jason , Hatton Joseph , Kim Jaewoo 
Published: October 2021

Abstract
Technology that facilitates estimation of individual animal intake rates in group-housed settings will result in improvements in animal production and management efficiency. Estimating intake in pasture settings may benefit from models that use other variables as proxies. Relationships among dry matter intake (DMI), animal performance variables, and environmental variables to model DMI were investigated. 202 animals were studied in a drylot setting (153 bulls for 85 days and 55 steers for 55 days) using VYTELLE SENSETM In-Pen-Weighing and Feed-Intake nodes. A machine learning model was calibrated using: DMI, sex, age, full body weight, ADG, water intake, water visit frequency and duration. DMI was positively related to full body weight (r = 0.39, P &lt; 0.001), water intake (r=0.23, P &lt; 0.001), and ADG (r=0.18, P &lt; 0.001). In addition, DMI had significant but weak correlations with water visit frequency (r=0.031, P &lt; 0.001). DMI exhibited weak negative relationships with maximum air temperature (r=-0.094, P &lt; 0.001) maximum relative humidity (r=-0.056, P &lt; 0.001), net radiation (r=-0.040, P &lt; 0.001), and precipitation (r=-0.022, P &lt; 0.001). Weak positive relationships were observed between DMI and maximum wind speed (r=0.031, P &lt; 0.001) and direction (r=-0.022, P &lt; 0.001). The model was validated with resultant average RMSE of 1.06 kg for daily predicted DMI compared to measured daily DMI. In addition, when daily predicted DMI was averaged for each animal, the accuracy of model results improved with RMSE of 0.11 kg. Study results demonstrate that inclusion of water intake and animal performance variables improves predictive accuracy of DMI. Validating and refining the model used to predict DMI in drylots will facilitate future extrapolation to larger group field settings. Vytelle and its logo are trademarks of Vytelle, LLC.