bluuebunny/crossref_metadata_2025_split huggingface dataset:
MENGGUNAKAN MEDIA INTERNET DOCS.GOOGLE PADA POKOK BAHASAN REAKSI REDOKS KELAS X IPA DI SMAN 4 KOTA BENGKULU
Authors: Parade Aang , Handayani Dewi , Sumpono Sumpono 
Published: July 2021

[USING THE DOCS. GOOGLE INTERNET MEDIA ON THE REDOX REACTION SUBJECT IN CLASS X IPA SMAN 4 BENGKULU CITY ] This research aims to know the results of learning: students in the class who are not given the preparation of learning at home, knowing the results of the teaching of students in a given grade of preparatory study at home using internet media, knowing the Docs. Google the difference in the results of student learning in the classroom are not given preparation home study with the results of a survey of students in a provided a homework preparatory sign Docs. Google internet media. The methods used in this research was quasi-experimental research is the entire population with grade X IPA SMAN 4 city of Bengkulu, Sample research, i.e., class experiments and classroom control. The results of the study in this research result of students pretest and posttest. Based on the results of the research conducted in mind that there is a significant difference between the results from student learning in the classroom are not given preparation home study with the results of a survey of students in a given a homework preparation home study using internet media Docs. Google via t-tests using SPSS 16 program. The learning outcomes of students in a given grade of preparatory education at home using Google Docs. Indicates the average rating of 77.0 while learning results of students in the class who are not given a homework preliminary with the average value of 68.6. Student Learning Outcomes in a given class homework preparation using Docs. Google may provide better results on the subject of redox reactions.
UJI AKTIVITAS ASAP CAIR CANGKANG BUAH KARET (Hevea brassiliensis) DAN APLIKASINYA DALAM PENGHAMBATAN KETENGIKAN DAGING SAPI
Authors: Putri Haulia Dwi , Sumpono Sumpono , Nurhamidah Nurhamidah 
Published: July 2021

This research was aimed to know the degree of Fenol compound, total acid and the activity of liquid smoke of Rubber (Hevea brassiliensis) Seed Shell also the application in obstructing the rancidity of beef. The Fenol compound was determined by using Reagen Follon-Ciocalteu. The total acid was determined by using the method of titrated acids.The ability as the antioxidant was tested by DPPH method and the ability of obstructing the rancidity of beef was done by measuring the alteration of TBA value in beef as long as saved. The result was shown the fenol compound in liquid smoke of Rubber Seed Shell as much as 0,84 % and the acetic acid content was 4.725%. The antioxidant test was produced IC50 value as much as 101.27 ppm. According to the IC50 value which got from the test that the liquid smoke of Rubber Seed Shell were included in moderate category. The increment of the liquid smoke of Rubber Seed Shell were able to suppress the rancidity of beef during the storage time. It was marked by the decreasing of TBA value in beef as given in treatment A1 (liquid smoke 4% ) and A2 liquid smoke 6%. The treatment without the liquid smoke (A0) increased the TBA value from 0.05 mgMDA/Kg on day (0) to 0.615 mgMDA/Kg on day 6. The treatment of concentration liquid smoke 4% (A1) increased from 0.039 mgMDA/Kg on day (0) to 0.395 mgMDA/Kg on day 6. The treatment of concentration liquid smoke 6% (A2) increased from 0.031 mgMDA/Kg on day 0 to 0.209 mgMDA/Kg on day 6.
POTENSI SITOTOKSIK DAN ANTIBAKTERI EKSTRAK DAUN Laportea interrupta (L.) Chew (JELATANG AYAM) TERHADAP Staphylococcus aureus
Authors: Safitri Okti Mindi , Nurhamidah Nurhamidah , Amir Hermansyah 
Published: July 2021

This research aims to determine the profile of phytochemicals, determine the level of cytotoxic extract toward the larva of Artemia salina Leach by using Brine Shrimp Lethality Test (BSLT) method, and to measure how much the activity of antibacteria leaf ekstract of Laportea Interrupta (L.) Chew (Jelatang Ayam ) toward the Staphylococcus aureus bacteria. The leaf of L. interrupta (L.) Chew are dried and grinded well. The half of grinded sample are tested by phytochemical profile and another one are ekstracted by using etanol liquid for three days then they are evaporated. The result of fitokimia leaf L. interrupta (L.) Chew test contains secondary metabolite alkaloid, tanin, terpenoid, and saponin. The result of cytotoxic extract of leaf L. interrupta (L.) Chew toward the A. salina Leach is obtained LC50 for 93,33 ppm, so, the L. interrupta (L.) Chew is toxic because it is in the range of 31 ppm to 1000 ppm and it can be potentially as a anticancer agents. The eksract of Jelatang Ayam leaf can obstruct the growth of S. aureus, on 5 x 104 clear zone concentration that formed 9 mm being medium categorized. The more concentration of the Jelatang leaf ekstract then more its obstruction energy.
STUDI PERBANDINGAN KEMAMPUAN BERPIKIR KRITIS SISWA MENGGUNAKAN MODEL KOOPERATIF TIPE TAKE AND GIVE DAN THINK PAIR SQUARE DI KELAS XI IPA SMA NEGERI 1 REJANG LEBONG
Authors: Amalia Windy Novita , Amir Hermansyah , Sumpono Sumpono 
Published: July 2021

The purpose of this research is to measure the difference of improvement of students' critical thinking ability by applying the cooperative model Take and give type with Think Pair Square type on salt hydrolysis material in grade XI IPA SMA Negeri 1 Rejang Lebong academic year 2017 / 2018. This study was conducted with a population of 201 students from 6 IPA classes and a sample of 68 students from 2 classes it class XI IPA 1 as experiment 1 and XI IPA 5 as experiment 2 which can be from normality and homogeneity test. The research is a kind of quasi experiment. Data analysis used is normality test, homogeneity test and hypothesis test. The students' critical thinking ability data is obtained from the pretest and posttest values at the beginning of the meeting and at the end of the meeting. This critical thinking skills test consists of 15 essay questions. The average increase in critical thinking skills for Take and give classes with Think Pair Square class is 44,757 and 51,683. By testing the hypothesis using t test with a significant level of 0.05, then obtained the value for t hitung> t table is 3.081> 1.668. The results showed that students' critical thinking skills with the classes that apply the Think Pair Square learning model is better than the class that applied the Take and give learning model.
PROFIL FITOKIMIA DAN PENGARUH EKSTRAK TANGKAI DAUN TALAS KEMUMU (Colocasia gigantea Hook.f ) TERHADAP JUMLAH LEUKOSIT Mus musculus
Authors: Asri Rofiqa , Handayani Dewi , Sundaryono Agus 
Published: July 2021

This research aims to identify secondary metabolite compounds in Talas kemumu leaf stalk (Colocasia gigantea Hook.f) and to determine the effect of extract on the number of male Mus musculus leukocytes that have been induced aspirin. Aspirin is used to increase the number of M.musculus leucocytes.The method used in this research is the method of extraction by maceration to attract secondary metabolites of active compounds C.gigantea leaf stalk and the measurement of blood leukocyte counts are calculated using a hemocytometer (improved neubauer). Test of the effect of the leaf stalk of C.giganteaextract on the number leukocytes of M.musculus was performed by dividing into 5 groups with 5 replications according to the Federer formula, namely K (-) were given distilled water, K (+) were given aspirin, P1, P2 and P3 are each given a dose of extract of C.gigantea leaf stalk of 0,028 g/KgBB, 0,056 g/KgBB and 0,084 g/KgBB. The results showed that the identification of phytochemical profile in C.gigantea leaf stalk contains secondary metabolite compounds such as alkaloids, flavonoids, terpenoids, tannins and saponins. The results shows that giving of C.gigantea leaf stalk extract can reduce the number of leukocytes M.musculus which have induced aspirin. The giving of C.gigantea leaf stalk extract can decrease the number of aspirin-induced M.musculus leukocytes. This is evident from the results of One Way Anova analysis and the Smallest Real Difference Test (BNT) because in each treatment group has a significant influence. The effective dose that has the greatest activity in decreasing the number of M.musculus leukocytes found in the P1 treatment of 0,028 g /KgBB can decrease the blood leukocyte count by 6,830 / mm3.
STUDY ON Hibiscus tiliaceus LEAVES AS ANTIBACTERIAL AND ANTIOXIDANT AGENTS
Authors: Samsudin Mohamad Syahmie , Andriani Yosie , Sarjono Purbowatiningrum Ria , Syamsumir Desy Fitrya 
Published: July 2021

This study aimed to evaluate antioxidant and antibacterial activities of methanol, hexane, dichloromethane, ethyl acetate, and water crude sample fractions of H. tiliaceus leaves. Various type of samples play an important role to determining which compound that will give the positive results in antioxidant test (inhibiting free radical scavenging activity) which they probably have potency for preventing the cancer disease. Furthermore, those types of samples were also tested to the antibacterial test as their possibilities to be created as new antibiotics in the future. This research also focused on what compound groups that actually present in H. tiliaceus leaves by using the phytochemistry test. Antioxidant potency of H. tiliaceus leaves extracts for the radical scavenging activity for quantitative assay was measured by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method. The antibacterial potency was measured by the Muller Hinton Agar (MHA) by applied the sample chemical crude in the plate. The result showed that all chemical crude of H. tiliaceus leaves has high antioxidant property as methanol crude (MC) 308.416%; hexane crude (HC) 232.837%; dichloromethane crude (DC) 150.837%; ethyl acetate crude (EC) 73.623% and water crude (WC) 71.777% with respected to the readings of 100% from quercetin (Q) as a positive control. From antibacterial result, entire samples had shown the positive results towards both gram positive (Staphylococcus aureus and Bacillus subtilis) and gram negative bacteria (Klebsiella pneumonia and Escherichia coli). The ranges of the inhibition zone were both at 7-10 mm and 10-15 mm. The phytochemistry test determine that the samples actually contained a lot of compounds which were proteins, carbohydrates, phenols/tannins, flavonoid, terpenoids, glycosides and also steroid. It is clearly indicated that H. tiliaceus leaves have potential to be used against the antioxidant and also as new antibacterial agents
Adaptaciones en la realización televisiva del deporte en directo por la COVID-19
Authors: Marín Montín Joaquín 
Published: July 2021

La irrupción del COVID-19 ha afectado a la producción televisiva de contenidos en directo. Los eventos deportivos televisados han alterado sus calendarios y su reanudación ha sido condicionada a la evolución de la pandemia. En la mayoría de los casos, se suprime la presencia del público en los recintos deportivos. Las principales competiciones han desarrollado modificaciones en su cobertura televisiva sujeta a las disposiciones de las autoridades sanitarias. Este artículo tiene como objetivo examinar las adaptaciones desarrolladas en la realización televisiva de un conjunto representativo de eventos deportivos durante la crisis sanitaria. También tiene como objetivo identificar cómo las innovaciones aportadas afectan a la recepción de los contenidos. La metodología aplicada combinó el estudio de caso y el análisis de contenido. Mediante el primero, se buscó tomar como referencia dos casos específicos de adaptaciones televisivas: competiciones europeas de fútbol y la NBA. En cuanto al segundo, el tratamiento de los datos seleccionados fue organizado en categorías a partir de las innovaciones encontradas en su retransmisión televisiva. Los resultados obtenidos indican cómo la incorporación de nuevos elementos durante la emisión televisiva de los grandes eventos deportivos permitió reforzar la experiencia de la audiencia, sobre todo con un incremento de recursos virtuales.
Type II Collagen Sponges Facilitate Tendon Stem/Progenitor Cells to Adopt More Chondrogenic Phenotypes and Promote the Regeneration of Fibrocartilage-Like Tissues in a Rabbit Partial Patellectomy Model
Authors: Wang Wen , Qin Shengnan , He Peiliang , Mao Wei , Chen Liang , Hua Xing , Zhang Jinli , Xiong Xifeng , Liu Zhihe , Wang Pengzhen , Meng Qingqi , Dong Fei , Li Aiguo , Chen Honghui , Xu Jiake 
Published: July 2021

ObjectiveFibrocartilage transition zone (FC) is difficult to regenerate after surgical re-attachment of tendon to bone. Here, we investigated whether type II collagen-sponges (CII-sponges) facilitated tendon stem/progenitor cells (TSPCs) to adopt chondrogenic phenotypes and further observed if this material could increase the FC areas in bone-tendon junction (BTJ) injury model.MethodsCII-sponges were made as we previously described. The appearance and pore structure of CII-sponges were photographed by camera and microscopies. The viability, proliferation, and differentiation of TSPCs were examined by LIVE/DEAD assay, alamarBlue, and PKH67 in vitro tracking. Subsequently, TSPCs were seeded in CII-sponges, Matrigel or monolayer, and induced under chondrogenic medium for 7 or 14 days before being harvested for qPCR or being transplanted into nude mice to examine the chondrogenesis of TSPCs. Lastly, partial patellectomy (PP) was applied to establish the BTJ injury model. CII-sponges were interposed between the patellar fragment and tendon, and histological examination was used to assess the FC regeneration at BTJ after surgery at 8 weeks.ResultsCII-sponges were like sponges with interconnected pores. TSPCs could adhere, proliferate, and differentiate in this CII-sponge up to 14 days at least. Both qPCR and immunostaining data showed that compared with TSPCs cultured in monolayer or Matrigel, cells in CII-sponges group adopted more chondrogenic phenotypes with an overall increase of chondrocyte-related genes and proteins. Furthermore, in PP injured model, much more new formed cartilage-like tissues could be observed in CII-sponges group, evidenced by a large amount of positive proteoglycan expression and typical oval or round chondrocytes in this area.ConclusionOur study showed that CII-sponges facilitated the TSPCs to differentiate toward chondrocytes and increased the area of FCs, which suggests that CII-sponges are meaningful for the reconstruction of FC at bone tendon junction. However, the link between the two phenomena requires further research and validation.
Genetic Polymorphisms of Long Non-coding RNA Linc00312 Are Associated With Susceptibility and Predict Poor Survival of Nasopharyngeal Carcinoma
Authors: Guo Zhen , Bao Mei-Hua , Fan Yun-Xia , Zhang Yan , Liu Hai-Yan , Zhou Xiao-Long , Wu Ben , Lu Qing-Qing , He Bin-Sheng , Nan Xu-Ying , Lu Jiao-Yang 
Published: July 2021

BackgroundLinc00312 is dysregulated in nasopharyngeal carcinoma (NPC) and participates in the initiation and progression of NPC. Our previous studies suggested that linc00312 was able to enhance the sensitivity of NPC cells to irradiation and NPC patients with higher expression of linc00312 was associated with better short-term curative effect and overall survival. The single nucleotide polymorphisms (SNPs) of lncRNAs may influence the disease course and outcome by affecting the expression, secondary structure or function of lncRNAs. However, the role of SNPs in linc00312 on the occurrence and survival of NPC remains unknown.MethodsWe recruited 684 NPC patients and 823 healthy controls to evaluate the association between linc00312 SNPs and NPC susceptibility by using multivariate logistic regression analysis. Kaplan-Meier analysis and Cox proportional hazards regression were applied to assess the effect of linc00312 SNPs on the survival of NPC patients. The relative expression of linc00312 in NPC tissues was determined by real-time PCR. The interaction between linc00312 and mir-411-3p was explored by luciferase reporter assay. In silico prediction of the changes on linc00312 folding structure was conducted by RNAfold WebServer.ResultWe demonstrated that rs12497104 (G &gt; A) GA genotype carriers had a higher risk than others for suffering from NPC (GA vs GG, OR = 1.437, P = 0.003). Besides, patients with rs12497104 AA genotype showed a poorer overall survival in contrast to GG genotype (AA vs GG, HR = 2.117, P = 0.011). In addition, the heterozygous carriers of rs15734 (G &gt; A) and rs164966 (A &gt; G) were correlated with decreased risk of NPC (GA vs GG, OR = 0.778, P = 0.031; GA vs AA, OR = 0.781, P = 0.033, respectively). We found that the three SNPs might influence the expression of linc00312 in a genotype specific feature. The local centroid secondary structure as well as the minimum free energy of linc00312 were changed following the candidate SNPs alterations. Besides, we revealed that the G to A alteration at rs12497104 disrupted the binding between mir-411-3p and linc00312.ConclusionOur results indicated genetic polymorphisms of linc00312 might serve as potential biomarkers for NPC carcinogenesis and prognosis.
Gut–Liver Axis: Liver Sinusoidal Endothelial Cells Function as the Hepatic Barrier in Colitis-Induced Liver Injury
Authors: Wang Yang , Zhang Yifan , Liu Yun , Xu Jun , Liu Yulan 
Published: July 2021

BackgroundBased on the gut–liver axis theory, a leaky gut can aggravate liver injury. However, clinical studies suggest that although gut mucosa damage is commonly observed in inflammatory bowel disease (IBD), it seldom leads to severe liver injury. We hypothesize that there is a hepatic barrier in the gut–liver axis, which protects the liver against gut-derived invasive factors.MethodsColitis was induced by dextran sulfate sodium (DSS) in eight different liver injury models in Sprague–Dawley rats. Liver sinusoidal endothelial cell (LSEC) injury was evaluated by a scanning and transmission electron microscope. Neutrophils were depleted by injection of anti-rat polymorphonuclear serum. Two pneumonia models were also induced to investigate the mechanism of neutrophil recruitment and activation. LSECs isolated from rat liver were used to investigate the effect on neutrophil recruitment and activation.ResultsAmong eight liver injury models, DSS colitis had no effect on liver injury in three models with normal LSECs. In the other five models with LSEC rupture, liver injury was significantly exacerbated by colitis, and increased hepatic neutrophil accumulation was observed. When neutrophils were depleted, colitis-induced liver injury was significantly attenuated. In pneumonia, liver injury, and colitis models, the level of CXCL1 correlated with the recruitment of neutrophils in different tissues, while DSS colitis and LSEC injury synergistically contributed to increased CXCL1 expression in the liver. In colitis-induced liver injury, neutrophils were activated in the liver. Injured LSECs showed both structural and functional changes, with significantly increased expression of CXCL1 and TNF-α under the stimulation of lipopolysaccharide (LPS). The combination of gut-derived LPS and LSEC-derived TNF-α led to the activation of neutrophils, characterized by enhanced production of reactive oxygen species, pro-inflammatory cytokines, and the formation of neutrophil extracellular traps.ConclusionLSECs constitute a vitally important barrier in the gut–liver axis, defending the liver against colitis-induced injury. When LSECs are damaged, they can turn into a pro-inflammatory pattern under the stimulation of LPS. LSEC injury and colitis-derived LPS synergistically contribute to the recruitment and activation of hepatic neutrophils. Neutrophils play a pivotal role as a downstream effector in colitis-induced liver injury.
Identification of a Novel Prognostic Signature of Genome Instability-Related LncRNAs in Early Stage Lung Adenocarcinoma
Authors: Peng Bo , Li Huawei , Na Ruisi , Lu Tong , Li Yongchao , Zhao Jiaying , Zhang Han , Zhang Linyou 
Published: July 2021

BackgroundIncreasing evidence has demonstrated that long non-coding RNAs (lncRNAs) play a crucial part in maintaining genomic instability. We therefore identified genome instability-related lncRNAs and constructed a prediction signature for early stage lung adenocarcinoma (LUAD) as well in order for classification of high-risk group of patients and improvement of individualized therapies.MethodsEarly stage LUAD RNA-seq and clinical data from The Cancer Genome Atlas (TCGA) were randomly divided into training set (n = 177) and testing set (n = 176). A total of 146 genomic instability-associated lncRNAs were identified based on somatic mutation profiles combining lncRNA expression profiles from TCGA by the “limma R” package. We performed Cox regression analysis to develop this predictive indicator. We validated the prognostic signature by an external independent LUAD cohort with microarray platform acquired from the Gene Expression Omnibus (GEO).ResultsA genome instability-related six-lncRNA-based gene signature (GILncSig) was established to divide subjects into high-risk and low-risk groups with different outcomes at statistically significant levels. According to the multivariate Cox regression and stratification analysis, the GILncSig was an independent predictive factor. Furthermore, the six-lncRNA signature achieved AUC values of 0.745, 0.659, and 0.708 in the training set, testing set, and TCGA set, respectively. When compared with other prognostic lncRNA signatures, the GILncSig also exhibited better prediction performance.ConclusionThe prognostic lncRNA signature is a potent tool for risk stratification of early stage LUAD patients. Our study also provided new insights for identifying genome instability-related cancer biomarkers.