bluuebunny/crossref_metadata_2025_split huggingface dataset:
Lightning activity in Yakutia and its impact on the networks of PJSC Yakutskenergo
Authors: Kozlov V I , Tarabukina L D , Grigor’ev Yu M 
Published: September 2021

Abstract
It is shown that daily, seasonal and interannual variations in the number of lightning outages in the Central and Western energy district regions of Yakutia correspond to variations in lightning activity in the area where these networks are located. The annual number of lightning outages was from 35 to 65. The daily variation has the form of a one-period wave with a maximum at 19:00 local time and a minimum at 7:00 LT. In the total diurnal variation over 7 years around 7 LT, about two outages were observed, while at 19 LT the total number reached 25. The similarity of daily, annual, and interannual variations in lightning activity and the number of thunderstorm outages indicates the absence of special features in the discharges that lead to thunderstorm outages of high-voltage networks.
Comparative analysis of the parameters of two radars MRL-5 and DMRL-C
Authors: Liev K B , Inyukhin V S , Kh Gergokov A 
Published: September 2021

Abstract
The scope of application of radar technology is currently very wide, and with the use of the achievements of modern circuitry, electronic technology and computer technology, it is expanding more and more. Therefore, consideration of the basic principles used in the construction of radar devices and systems is highly relevant. Radio technical equipment and means intended for performing radar tasks are called radar systems, or devices. In this article, we will consider two meteorological radars MRL-5 with the installed software and hardware complex ASU-MRL and DMRL-C with different wavelengths λ=10 cm and λ=5.2 cm. And also consider the assessment of the possibility of using information from the Unified radar field of the Roshydromet DMRL-C network to ensure work on protection against hail, including technical requirements for radar information of the DMRL-C for its use in work on AB on the territory of the South and North Caucasian Federal Districts. The first section in your paper.
Two-stage-vote ensemble framework based on integration of mutation data and gene interaction network for uncovering driver genes
Authors: Kan Yingxin , Jiang Limin , Guo Yan , Tang Jijun , Guo Fei 
Published: September 2021

Abstract
Identifying driver genes, exactly from massive genes with mutations, promotes accurate diagnosis and treatment of cancer. In recent years, a lot of works about uncovering driver genes based on integration of mutation data and gene interaction networks is gaining more attention. However, it is in suspense if it is more effective for prioritizing driver genes when integrating various types of mutation information (frequency and functional impact) and gene networks. Hence, we build a two-stage-vote ensemble framework based on somatic mutations and mutual interactions. Specifically, we first represent and combine various kinds of mutation information, which are propagated through networks by an improved iterative framework. The first vote is conducted on iteration results by voting methods, and the second vote is performed to get ensemble results of the first poll for the final driver gene list. Compared with four excellent previous approaches, our method has better performance in identifying driver genes on $33$ types of cancer from The Cancer Genome Atlas. Meanwhile, we also conduct a comparative analysis about two kinds of mutation information, five gene interaction networks and four voting strategies. Our framework offers a new view for data integration and promotes more latent cancer genes to be admitted.
Assessment of hypertension in kidney transplantation by ambulatory blood pressure monitoring: a systematic review and meta-analysis
Authors: Pisano Anna , Mallamaci Francesca , D’Arrigo Graziella , Bolignano Davide , Wuerzner Gregoire , Ortiz Alberto , Burnier Michel , Kanaan Nada , Sarafidis Pantelis , Persu Alexandre , Ferro Charles J , Loutradis Charalampos , Boletis Ioannis N , London Gérard , Halimi Jean-Michel , Sautenet Bénédicte , Rossignol Patrick , Vogt Liffert , Zoccali Carmine 
Published: September 2021

ABSTRACT

Background
Hypertension (HTN) is common following renal transplantation and it is associated with adverse effects on cardiovascular (CV) and graft health. Ambulatory blood pressure monitoring (ABPM) is the preferred method to characterize blood pressure (BP) status, since HTN misclassification by office BP (OBP) is quite common in this population. We performed a systematic review and meta-analysis aimed at determining the clinical utility of 24-h ABPM and its potential implications for the management of HTN in this population.

Methods
Ovid-MEDLINE and PubMed databases were searched for interventional or observational studies enrolling adult kidney transplant recipients (KTRs) undergoing 24-h ABP readings compared with OBP or home BP. The main outcome was the proportion of KTRs diagnosed with HTN by ABPM, home or OBP recordings. Additionally, day–night BP variability and dipper/non-dipper status were assessed.

Results
Forty-two eligible studies (4115 participants) were reviewed. A cumulative analysis including 27 studies (3481 participants) revealed a prevalence of uncontrolled HTN detected by ABPM of 56% [95% confidence interval (CI) 46–65%]. The pooled prevalence of uncontrolled HTN according to OBP was 47% (95% CI 36–58%) in 25 studies (3261 participants). Very few studies reported on home BP recordings. The average concordance rate between OBP and ABPM measurements in classifying patients as controlled or uncontrolled hypertensive was 66% (95% CI 59–73%). ABPM revealed HTN phenotypes among KTRs. Two pooled analyses of 11 and 10 studies, respectively, revealed an average prevalence of 26% (95% CI 19–33%) for masked HTN (MHT) and 10% (95% CI 6–17%) for white-coat HTN (WCH). The proportion of non-dippers was variable across the 28 studies that analysed dipping status, with an average prevalence of 54% (95% CI 45–63%).

Conclusions
In our systematic review, comparison of OBP versus ABP measurements disclosed a high proportion of MHT, uncontrolled HTN and, to a lesser extent, WCH in KTRs. These results suggest that HTN is not adequately diagnosed and controlled by OBP recordings in this population. Furthermore, the high prevalence of non-dippers confirmed that circadian rhythm is commonly disturbed in KTRs.
Standardized Workflow for Precise Mid- and High-Throughput Proteomics of Blood Biofluids
Authors: Mc Ardle Angela , Binek Aleksandra , Moradian Annie , Chazarin Orgel Blandine , Rivas Alejandro , Washington Kirstin E , Phebus Conor , Manalo Danica-Mae , Go James , Venkatraman Vidya , Coutelin Johnson Casey W , Fu Qin , Cheng Susan , Raedschelders Koen , Fert-Bober Justyna , Pennington Stephen R , Murray Christopher I , Van Eyk Jennifer E 
Published: September 2021

Abstract

Background
Accurate discovery assay workflows are critical for identifying authentic circulating protein biomarkers in diverse blood matrices. Maximizing the commonalities in the proteomic workflows between different biofluids simplifies the approach and increases the likelihood for reproducibility. We developed a workflow that can accommodate 3 blood-based proteomes: naive plasma, depleted plasma and dried blood.

Methods
Optimal conditions for sample preparation and data independent acquisition-mass spectrometry analysis were established in plasma then automated for depleted plasma and dried blood. The mass spectrometry workflow was modified to facilitate sensitive high-throughput analysis or deeper profiling with mid-throughput analysis. Analytical performance was evaluated by the linear response of peptides and proteins to a 6- or 7-point dilution curve and the reproducibility of the relative peptide and protein intensity for 5 digestion replicates per day on 3 different days for each biofluid.

Results
Using the high-throughput workflow, 74% (plasma), 93% (depleted), and 87% (dried blood) displayed an inter-day CV &lt;30%. The mid-throughput workflow had 67% (plasma), 90% (depleted), and 78% (dried blood) of peptides display an inter-day CV &lt;30%. Lower limits of detection and quantification were determined for peptides and proteins observed in each biofluid and workflow. Based on each protein and peptide’s analytical performance, we could describe the observable, reliable, reproducible, and quantifiable proteomes for each biofluid and workflow.

Conclusion
The standardized workflows established here allows for reproducible and quantifiable detection of proteins covering a broad dynamic range. We envisage that implementation of this standard workflow should simplify discovery approaches and facilitate the translation of candidate markers into clinical use.
Thrombocytopaenia successfully treated by a multilocular thymic cyst resection
Authors: Nishina Mai , Mimura Takeshi , Kuraoka Kazuya , Yamashita Yoshinori 
Published: September 2021

Abstract
The multilocular thymic cyst (MTC) is a rare, acquired disease caused by inflammatory changes in the thymus, and is associated with autoimmune diseases. We report a case of MTC with thrombocytopaenia, which improved following surgical resection. A 45-year-old man developed thrombocytopaenia with an anterior mediastinal tumour. Thrombocytopaenia due to an autoimmune mechanism, associated with thymoma or thymus-related disease, was suspected. Pathologic analysis following thoracoscopic thymectomy confirmed MTC. The platelet level recovered postoperatively. Our findings suggested a relationship between the acquired formation of MTC and the development of autoimmune antibodies. However, further investigation is needed to obtain more information.
Multilingualism in Lebanon: bridging reality to practice
Authors: Zeaiter Lana 
Published: September 2021

Abstract
Although Lebanese use different languages, mainly Arabic, English, and French, in their daily interactions, the Lebanese educational system continues to adopt monolingual-oriented practices to language teaching. In English language classes, teachers and students are expected to exclusively use the target language as the language of instruction. Such a practice hinders students’ engagement in the learning process, especially during early stages, as they often face vocabulary and pronunciation challenges. Hence, there is a discrepancy between linguistic norms prevailing outside the classroom and the teaching strategies implemented inside the classroom. In an attempt to bridge the gap and maximize students’ English learning, I utilized my position as an English instructor to teach through the implementation of multilingual strategies. This article draws on my experience and on my students’ feedback to provide insights into implementing multilingual strategies to teach English language, with a focus on design and affordances of specific strategies.
Analytical strategies for chemical exposomics: exploring limits and feasibility
Authors: Vitale Chiara Maria , Price Elliott J , Miller Gary W , David Arthur , Antignac Jean-Philippe , Barouki Robert , Klánová Jana 
Published: September 2021

Abstract
Tackling the challenges of chemical exposomics will require the implementation of diverse analytical strategies and technological advancements. Herein, high-resolution mass spectrometry-based methods applied in current chemical exposome studies have been surveyed and are shown to be limited. Notably, liquid chromatography separations almost exclusively employ reversed-phase C18 columns using water-methanol gradients with formic acid additive, whilst gas chromatography is underexploited in the field at this stage. A systematic evaluation of strategies applied in related disciplines (i.e. metabolomics, proteomics, multi-residue trace analysis) was undertaken to provide practical guidance for the development of chemical exposomics. The approaches were assessed on the basis of their costs (i.e. capital expenditure, overhead and maintenance fees, expertise required, consumables) and potential benefits (i.e. improvements to sensitivity, coverage, reproducibility, throughput, ease of use) to prioritize those with promise for chemical exposomics application. Alongside a need for technological investments (e.g. advanced hardware updates), numerous low cost strategies showed high potential benefits (e.g. different column phases, enhanced sample fractionation) and are feasible for rapid adoption.
Two promoters integrate multiple enhancer inputs to drive wild-type knirps expression in the Drosophila melanogaster embryo
Authors: Li Lily , Waymack Rachel , Gad Mario , Wunderlich Zeba 
Published: September 2021

Abstract
Proper development depends on precise spatiotemporal gene expression patterns. Most developmental genes are regulated by multiple enhancers and often by multiple core promoters that generate similar transcripts. We hypothesize that multiple promoters may be required either because enhancers prefer a specific promoter or because multiple promoters serve as a redundancy mechanism. To test these hypotheses, we studied the expression of the knirps locus in the early Drosophila melanogaster embryo, which is mediated by multiple enhancers and core promoters. We found that one of these promoters resembles a typical “sharp” developmental promoter, while the other resembles a “broad” promoter usually associated with housekeeping genes. Using synthetic reporter constructs, we found that some, but not all, enhancers in the locus show a preference for one promoter, indicating that promoters provide both redundancy and specificity. By analyzing the reporter dynamics, we identified specific burst properties during the transcription process, namely burst size and frequency, that are most strongly tuned by the combination of promoter and enhancer. Using locus-sized reporters, we discovered that enhancers with no promoter preference in a synthetic setting have a preference in the locus context. Our results suggest that the presence of multiple promoters in a locus is due both to enhancer preference and a need for redundancy and that “broad” promoters with dispersed transcription start sites are common among developmental genes. They also imply that it can be difficult to extrapolate expression measurements from synthetic reporters to the locus context, where other variables shape a gene’s overall expression pattern.
The zinc cluster transcription factor Rha1 is a positive filamentation regulator in Candida albicans
Authors: Parvizi Omran Raha , Ramírez-Zavala Bernardo , Aji Tebung Walters , Yao Shuangyan , Feng Jinrong , Law Chris , Dumeaux Vanessa , Morschhäuser Joachim , Whiteway Malcolm 
Published: September 2021

Abstract
Zinc cluster transcription factors (TFs) are essential fungal regulators of gene expression. In the pathogen Candida albicans, the gene orf19.1604 encodes a zinc cluster TF regulating filament development. Hyperactivation of orf19.1604, which we have named RHA1 for Regulator of Hyphal Activity, generates wrinkled colony morphology under nonhyphal growth conditions, triggers filament formation, invasiveness, and enhanced biofilm formation and causes reduced virulence in the mouse model of systemic infection. The strain expressing activated Rha1 shows up-regulation of genes required for filamentation and cell-wall-adhesion-related proteins. Increased expression is also seen for the hyphal-inducing TFs Brg1 and Ume6, while the hyphal repressor Nrg1 is downregulated. Inactivation of RHA1 reduces filamentation under a variety of filament-inducing conditions. In contrast to the partial effect of either single mutant, the double rha1 ume6 mutant strain is highly defective in both serum- and Spider-medium-stimulated hyphal development. While the loss of Brg1 function blocks serum-stimulated hyphal development, this block can be significantly bypassed by Rha1 hyperactivity, and the combination of Rha1 hyperactivity and serum addition can generate significant polarization even in brg1 ume6 double mutants. Thus, in response to external signals, Rha1 functions with other morphogenesis regulators including Brg1 and Ume6, to mediate filamentation.
Lysosomes and the pathogenesis of merosin-deficient congenital muscular dystrophy
Authors: Smith Sarah J , Fabian Lacramioara , Sheikh Adeel , Noche Ramil , Cui Xiucheng , Moore Steven A , Dowling James J 
Published: September 2021

AbstractCongenital muscular dystrophy type 1A (MDC1A), the most common congenital muscular dystrophy in Western countries, is caused by recessive mutations in LAMA2, the gene encoding laminin alpha 2. Currently, no cure or disease modifying therapy has been successfully developed for MDC1A. Examination of patient muscle biopsies revealed altered distribution of lysosomes. We hypothesized that this redistribution was a novel and potentially druggable aspect of disease pathogenesis. We explored this hypothesis using candyfloss (caf), a zebrafish model of MDC1A. We found that lysosome distribution in caf zebrafish was also abnormal. This altered localization was significantly associated with fiber detachment and could be prevented by blocking myofiber detachment. Overexpression of transcription factor EB, a transcription factor that promotes lysosomal biogenesis, led to increased lysosome content and decreased fiber detachment. We conclude that genetic manipulation of the lysosomal compartment is able to alter the caf zebrafish disease process, suggesting that lysosome function may be a target for disease modification.
Strong evidence for genotype–phenotype correlations in Phelan-McDermid syndrome: results from the developmental synaptopathies consortium
Authors: Levy Tess , Foss-Feig Jennifer H , Betancur Catalina , Siper Paige M , Trelles-Thorne Maria del Pilar , Halpern Danielle , Frank Yitzchak , Lozano Reymundo , Layton Christina , Britvan Bari , Bernstein Jonathan A , Buxbaum Joseph D , Berry-Kravis Elizabeth , Powell Craig M , Srivastava Siddharth , Sahin Mustafa , Soorya Latha , Thurm Audrey , Kolevzon Alexander ,   the Developmental Synaptopathies Consortium
Published: September 2021

AbstractIndividuals with Phelan-McDermid syndrome (PMS) present with a wide range of developmental, medical, cognitive and behavioral abnormalities. Previous literature has begun to elucidate genotype–phenotype associations that may contribute to the wide spectrum of features. Here, we report results of genotype–phenotype associations in a cohort of 170 individuals with PMS. Genotypes were defined as Class I deletions (including SHANK3 only or SHANK3 with ARSA and/or ACR and RABL2B), Class II deletions (all other deletions) or sequence variants. Phenotype data were derived prospectively from direct evaluation, caregiver interview and questionnaires, and medical history. Analyses revealed individuals with Class I deletions or sequence variants had fewer delayed developmental milestones and higher cognitive ability compared to those with Class II deletions but had more skill regressions. Individuals with Class II deletions were more likely to have a variety of medical features, including renal abnormalities, spine abnormalities, and ataxic gait. Those with Class I deletions or sequence variants were more likely to have psychiatric diagnoses including bipolar disorder, depression, and schizophrenia. Autism spectrum disorder diagnoses did not differ between groups. This study represents the largest and most rigorous genotype–phenotype analysis in PMS to date and provides important information for considering clinical functioning, trajectories and comorbidities as a function of specific genetic alteration.
Successful treatment of atypical type A aortic dissection after heart transplantation
Authors: Shu Shenglei , Cheng Lan , Wang Jing , Zheng Chuansheng 
Published: September 2021

Abstract
We report a case of a 58-year-old female with Stanford type A aortic dissection sparring the donor aorta 2 years after heart transplantation. Lumbar spine magnetic resonance examination for low back pain discovered the aortic dissection which was confirmed by following computed tomography angiography. The patient received surgical treatment including total arc replacement and thoracic aortic endovascular repair and recovered well.
Compounded Cerium Nitrate–Silver Sulfadiazine Cream is Safe and Effective for the Treatment of Burn Wounds: A Burn Center’s 4-Year Experience
Authors: Reese Adam D , Keyloun John W , Garg Gaurav , McLawhorn Melissa M , Moffatt Lauren T , Travis Taryn E , Johnson Laura S , Shupp Jeffrey W 
Published: September 2021

Abstract
Wound infections and sepsis are significant causes of morbidity after burn injury and can be alleviated by early excision and grafting. In situations that preclude early surgery, topical agents allow for a safer delay. Cerium nitrate compounded with silver sulfadiazine (Ce-SSD) is a burn cream that provides broad antibacterial activity, forms a temporary barrier, and promotes re-epithelialization. Methemoglobinemia is a rare, but oft-cited, systemic complication of Ce-SSD. In this retrospective review, 157 patients treated with Ce-SSD between July 2014 and July 2018 were identified, and the monitoring protocol for methemoglobinemia during Ce-SSD treatment was evaluated. The median age was 59 years (interquartile range [IQR], 47–70.5 years), with TBSA of 8.5% (IQR, 3–27), adjusted Baux score of 76 (IQR, 59–94), and inhalation injury present in 9.9% of patients. Primary endpoints included incidence of symptomatic and asymptomatic methemoglobinemia. Of the 9.6% (n = 15) of patients with methemoglobinemia, 73.3% (n = 11) had maximum methemoglobin levels ≥72 hours from the time of the first application. One patient developed clinically significant methemoglobinemia. Patients with TBSA ≥20% were more likely to develop methemoglobinemia (odds ratio 9.318, 95% confidence interval 2.078–65.73, P = .0078); however, neither Ce-SSD doses nor days of exposure were significant predictors. Ce-SSD application to temporize burn wounds until excision and grafting is safe, effective, and, in asymptomatic patients with TBSA &lt;20%, can be used without serial blood gas monitoring. Vigilant monitoring for symptoms should be performed in patients with TBSA ≥20%, but routine blood gases are not necessary.
The role of vitamin K in the non-operative management of bilateral adrenal hemorrhage on a background of antiphospholipid syndrome and coagulopathy: a case report
Authors: Aneke Ikenna Anderson , Gunendran Thiruendran 
Published: September 2021

AbstractBilateral adrenal hemorrhage (BAH) is a rare condition, which can easily become a serious threat to life. It is even rarer in the setting of antiphospholipid syndrome (APLS), which in itself is associated with coagulopathy as well as the need for lifelong anticoagulation. Death from BAH is commonly due to adrenal insufficiency from infarction within the gland. Our case is that of a 53-year-old woman with APLS who presented to the emergency department with a markedly raised international normalized ratio and bilateral loin pain. She developed adrenal insufficiency and required glucocorticoids while on admission. Her anticoagulants were also adjusted and she received vitamin K. She made good progress thereafter and was discharged after 11 days. Vitamin K should be considered in patients with markedly deranged clotting profile and BAH to prevent ongoing bleeding. Early adrenal axis testing is also recommended to forestall delayed diagnosis of adrenal insufficiency.
Metachronous splenic metastasis from renal cell carcinoma: a case report
Authors: Moez Rahoui , Rami Boulma , Hassen Khouni 
Published: September 2021

AbstractThe causes of isolated solid splenic lesions are wide and varied, and as such can present a diagnostic challenge. Splenic metastases were previously considered exceptionally rare. We report a case of a patient who had isolated splenic metastases with a previous history of left nephrectomy due to a renal cell carcinoma 3 years before. This report represents the first case reported in our country and wants to add to literature one more case of splenic metastasis from renal cell carcinoma.