bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effects of fertilizer under different dripline spacings on summer maize in northern China
Authors: Li Ge , Bai Youlu , Wang Lei , Lu Yanli , Zhang Jingjing , Zhang Yinjie 
Published: September 2021

AbstractMaximizing grain yields with effective fertilization technologies and minimizing nitrogen losses is essential in agroecosystems. In this research, we conducted a two-year field experiment to explore whether dripline spacing and fertilization rate would affect maize grain yield. Two dripline spacings (i.e., one drip line per row of maize with a row space of 60 cm and one drip line per two rows of maize) and two fertilization rates (i.e., high fertilization level: N, 180 kg ha−1; P2O5, 90 kg ha−1; and K2O, 90 kg ha−1 and low level: N, 139.5 kg ha−1; P2O5, 76.5 kg ha−1; and K2O, 76.5 kg ha−1) were employed in this research. The results showed that maize yield was significantly affected by both dripline spacing and fertilization rate. The maize yield was 10.2% higher in the treatment with one drip line per two rows than that in the treatment with one drip line per row. Maize yield increased by 10.9% at the high fertilization level compared to that at the low fertilization level. The quantity of cumulative ammonia volatilization was reduced by 15.1% with one drip line per two rows compared to that with one drip line per row, whereas it increased by 26.9% at the high fertilization level compared with that at the low fertilization level. These results indicated that one drip line per two rows with a high fertilization rate increased the yield and could reduce the environmental burden, which may be economically beneficial and environmentally sound for maize fertigation for green agricultural development.
The Charlson Comorbidity Index is associated with risk of 30-day mortality in patients with myocardial injury after non-cardiac surgery
Authors: Kim Sojin , Park Jungchan , Kwon Ji-Hye , Oh Ah Ran , Gook Joonhee , Yang Kwangmo , Choi Jin-ho , Kim Kyunga , Sung Ji Dong , Ahn Joonghyun , Lee Seung-Hwa 
Published: September 2021

AbstractMyocardial injury after non-cardiac surgery (MINS) is a well-known and relevant indicator of early postoperative mortality, but factors related to increased mortality in MINS patients are as yet unknown. The Charlson Comorbidity Index (CCI) is widely used to classify various comorbid conditions and underlying diseases. Our study aimed to determine the prognostic value of CCI with regard to mortality of patients with MINS. This study comprises 5633 patients who had MINS as diagnosed by a rise of postoperative cardiac troponin I above the normal range (≥ 0.04 ng/mL) from January 2010 to June 2019. Patients were divided into two groups according to median weighted CCI score: low CCI (≤ 2) and high CCI (> 2) groups. The primary outcome was 30-day mortality after surgery, and secondary outcomes were 1-year and overall mortalities. Of the 5633 patients, 3428 (60.9%) were in the low CCI group (1.21 ± 0.84) and 2205 (39.1%) were in the high CCI group (4.17 ± 1.82). After propensity score matching, mortality during the first 30 days after surgery was significantly greater in the high CCI group than the low CCI group (9.4% vs. 6.0%, respectively; hazard ratio 1.56, 95% confidence interval 1.23–1.98, p < 0.001). A high CCI score was associated with increased 30-day mortality in patients with MINS, suggesting that the CCI may need to be considered when predicting outcomes of MINS patients.
Role of pyruvate in maintaining cell viability and energy production under high-glucose conditions
Authors: Yako Hideji , Niimi Naoko , Kato Ayako , Takaku Shizuka , Tatsumi Yasuaki , Nishito Yasumasa , Kato Koichi , Sango Kazunori 
Published: September 2021

AbstractPyruvate functions as a key molecule in energy production and as an antioxidant. The efficacy of pyruvate supplementation in diabetic retinopathy and nephropathy has been shown in animal models; however, its significance in the functional maintenance of neurons and Schwann cells under diabetic conditions remains unknown. We observed rapid and extensive cell death under high-glucose (> 10 mM) and pyruvate-starved conditions. Exposure of Schwann cells to these conditions led to a significant decrease in glycolytic flux, mitochondrial respiration and ATP production, accompanied by enhanced collateral glycolysis pathways (e.g., polyol pathway). Cell death could be prevented by supplementation with 2-oxoglutarate (a TCA cycle intermediate), benfotiamine (the vitamin B1 derivative that suppresses the collateral pathways), or the poly (ADP-ribose) polymerase (PARP) inhibitor, rucaparib. Our findings suggest that exogenous pyruvate plays a pivotal role in maintaining glycolysis–TCA cycle flux and ATP production under high-glucose conditions by suppressing PARP activity.
Persistence and accumulation of environmental DNA from an endangered dragonfly
Authors: Schmidt Kristie J. , Soluk Daniel A. , Maestas Sarah E. Mays , Britten Hugh B. 
Published: September 2021

AbstractDetection of environmental DNA (eDNA) has become a commonly used surveillance method for threatened or invasive vertebrates in both aquatic and terrestrial environments. However, most studies in this field favor vertebrate target species. Environmental DNA protocols can be especially useful for endangered invertebrates such as the Hine’s emerald dragonfly (Somatochlora hineana) where conservation efforts have been greatly hindered by training, time, overall costs, and environmental impacts associated with conducting surveys in the calcareous fens occupied by this species. An essential step in developing such a protocol is to evaluate the dynamics of eDNA concentration under controlled conditions. We used the quantitative polymerase chain reaction (qPCR) to examine seasonal shifts in the persistence and net-accumulation of eDNA from captive S. hineana larvae in experimental mesocosms at temperatures corresponding with their overwintering (5.0 °C) and active (16.0 °C) seasons. Environmental DNA persisted longer at 5.0 °C but accumulated more readily at 16.0 °C. Differences in the accumulation and persistence of eDNA reflect differences in the longevity of eDNA at different temperatures and seasonal differences in larval S. hineana behavior. This study highlights the importance of considering how seasonal changes in temperature influence not only the speed of eDNA degradation but also the target species’ eDNA shedding rates.
Factors relating to mortality in septic patients in Vietnamese intensive care units from a subgroup analysis of MOSAICS II study
Authors: Do Son Ngoc , Luong Chinh Quoc , Pham Dung Thi , Nguyen My Ha , Nguyen Nga Thi , Huynh Dai Quang , Hoang Quoc Trong Ai , Dao Co Xuan , Le Trung Minh , Bui Ha Nhat , Nguyen Hung Tan , Hoang Hai Bui , Le Thuy Thi Phuong , Nguyen Lien Thi Bao , Duong Phuoc Thien , Nguyen Tuan Dang , Vu Yen Hai , Pham Giang Thi Tra , Van Bui Tam , Pham Thao Thi Ngoc , Hoang Hanh Trong , Van Bui Cuong , Nguyen Nguyen Minh , Bui Giang Thi Huong , Vu Thang Dinh , Le Nhan Duc , Tran Trang Huyen , Nguyen Thang Quang , Le Vuong Hung , Van Nguyen Chi , McNally Bryan Francis , Phua Jason , Nguyen Anh Dat 
Published: September 2021

AbstractSepsis is the most common cause of in-hospital deaths, especially from low-income and lower-middle-income countries (LMICs). This study aimed to investigate the mortality rate and associated factors from sepsis in intensive care units (ICUs) in an LMIC. We did a multicenter cross-sectional study of septic patients presenting to 15 adult ICUs throughout Vietnam on the 4 days representing the different seasons of 2019. Of 252 patients, 40.1% died in hospital and 33.3% died in ICU. ICUs with accredited training programs (odds ratio, OR: 0.309; 95% confidence interval, CI 0.122–0.783) and completion of the 3-h sepsis bundle (OR: 0.294; 95% CI 0.083–1.048) were associated with decreased hospital mortality. ICUs with intensivist-to-patient ratio of 1:6 to 8 (OR: 4.533; 95% CI 1.621–12.677), mechanical ventilation (OR: 3.890; 95% CI 1.445–10.474) and renal replacement therapy (OR: 2.816; 95% CI 1.318–6.016) were associated with increased ICU mortality, in contrast to non-surgical source control (OR: 0.292; 95% CI 0.126–0.678) which was associated with decreased ICU mortality. Improvements are needed in the management of sepsis in Vietnam such as increasing resources in critical care settings, making accredited training programs more available, improving compliance with sepsis bundles of care, and treating underlying illness and shock optimally in septic patients.
Cytochrome P450 isoforms 1A1, 1B1 AND 2W1 as targets for therapeutic intervention in head and neck cancer
Authors: Presa Daniela , Khurram Syed A. , Zubir Amir Z. A. , Smarakan Sneha , Cooper Patricia A. , Morais Goreti R. , Sadiq Maria , Sutherland Mark , Loadman Paul M. , McCaul James , Shnyder Steven D. , Patterson Laurence H. , Pors Klaus 
Published: September 2021

AbstractEpidemiological studies have shown that head and neck cancer (HNC) is a complex multistage process that in part involves exposure to a combination of carcinogens and the capacity of certain drug-metabolising enzymes including cytochrome P450 (CYP) to detoxify or activate such carcinogens. In this study, CYP1A1, CYP1B1 and CYP2W1 expression in HNC was correlated with potential as target for duocarmycin prodrug activation and selective therapy. In the HNC cell lines, elevated expression was shown at the gene level for CYP1A1 and CYP1B1 whereas CYP2W1 was hardly detected. However, CYP2W1 was expressed in FaDu and Detroit-562 xenografts and in a cohort of human HNC samples. Functional activity was measured in Fadu and Detroit-562 cells using P450-Glo™ assay. Antiproliferative results of duocarmycin prodrugs ICT2700 and ICT2706 revealed FaDu and Detroit-562 as the most sensitive HNC cell lines. Administration of ICT2700 in vivo using a single dose of ICT2700 (150 mg/kg) showed preferential inhibition of small tumour growth (mean size of 60 mm3) in mice bearing FaDu xenografts. Significantly, our findings suggest a potential targeted therapeutic approach to manage HNCs by exploiting intratumoural CYP expression for metabolic activation of duocarmycin-based prodrugs such as ICT2700.
Emergence of universality in the transmission dynamics of COVID-19
Authors: Paul Ayan , Bhattacharjee Jayanta Kumar , Pal Akshay , Chakraborty Sagar 
Published: September 2021

AbstractThe complexities involved in modelling the transmission dynamics of COVID-19 has been a roadblock in achieving predictability in the spread and containment of the disease. In addition to understanding the modes of transmission, the effectiveness of the mitigation methods also needs to be built into any effective model for making such predictions. We show that such complexities can be circumvented by appealing to scaling principles which lead to the emergence of universality in the transmission dynamics of the disease. The ensuing data collapse renders the transmission dynamics largely independent of geopolitical variations, the effectiveness of various mitigation strategies, population demographics, etc. We propose a simple two-parameter model—the Blue Sky model—and show that one class of transmission dynamics can be explained by a solution that lives at the edge of a blue sky bifurcation. In addition, the data collapse leads to an enhanced degree of predictability in the disease spread for several geographical scales which can also be realized in a model-independent manner as we show using a deep neural network. The methodology adopted in this work can potentially be applied to the transmission of other infectious diseases and new universality classes may be found. The predictability in transmission dynamics and the simplicity of our methodology can help in building policies for exit strategies and mitigation methods during a pandemic.
Polygenic risk for obsessive-compulsive disorder (OCD) predicts brain response during working memory task in OCD, unaffected relatives, and healthy controls
Authors: Heinzel Stephan , Kaufmann Christian , Grützmann Rosa , Klawohn Julia , Riesel Anja , Bey Katharina , Heilmann-Heimbach Stefanie , Weinhold Leonie , Ramirez Alfredo , Wagner Michael , Kathmann Norbert 
Published: September 2021

AbstractAlterations in frontal and parietal neural activations during working memory task performance have been suggested as a candidate endophenotype of obsessive-compulsive disorder (OCD) in studies involving first-degree relatives. However, the direct link between genetic risk for OCD and neuro-functional alterations during working memory performance has not been investigated to date. Thus, the aim of the current functional magnetic resonance imaging (fMRI) study was to test the direct association between polygenic risk for OCD and neural activity during the performance of a numeric n-back task with four working memory load conditions in 128 participants, including patients with OCD, unaffected first-degree relatives of OCD patients, and healthy controls. Behavioral results show a significant performance deficit at high working memory load in both patients with OCD and first-degree relatives (p < 0.05). A whole-brain analysis of the fMRI data indicated decreased neural activity in bilateral inferior parietal lobule and dorsolateral prefrontal cortex in both patients and relatives. Most importantly, OCD polygenic risk scores predicted neural activity in orbitofrontal cortex. Results indicate that genetic risk for OCD can partly explain alterations in brain response during working memory performance, supporting the notion of a neuro-functional endophenotype for OCD.
First microsatellite markers for the European Robin (Erithacus rubecula) and their application in analysis of parentage and genetic diversity
Authors: Gwiazdowska Aleksandra , Karpińska Oliwia , Kamionka-Kanclerska Katarzyna , Rowiński Patryk , Panagiotopoulou Hanna , Pomorski Jan J. , Broughton Richard K. , da Silva Luis F. P. , Rutkowski Robert 
Published: September 2021

AbstractThe European Robin is a small passerine bird associated with woodlands of Eurasia and North Africa. Despite being relatively widespread and common, little is known of the species’ breeding biology and genetic diversity. We used Next Generation Sequencing (NGS) to develop and characterize microsatellite markers for the European Robin, designing three multiplex panels to amplify 14 microsatellite loci. The level of polymorphism and its value for assessing parentage and genetic structure was estimated based on 119 individuals, including seven full families and 69 unrelated individuals form Poland’s Białowieża Primaeval Forest and an additional location in Portugal. All markers appeared to be highly variable. Analysis at the family level confirmed a Mendelian manner of inheritance in the investigated loci. Genetic data also revealed evidence for extra-pair paternity in one family. The set of markers that we developed are proven to be valuable for analysis of the breeding biology and population genetics of the European Robin.
Endometrial microbiota is more diverse in people with endometriosis than symptomatic controls
Authors: Wessels Jocelyn M. , Domínguez Miguel A. , Leyland Nicholas A. , Agarwal Sanjay K. , Foster Warren G. 
Published: September 2021

AbstractEndometriosis is a chronic, estrogen-dependent gynecological condition affecting approximately 10% of reproductive age women. The most widely accepted theory of its etiology includes retrograde menstruation. Recent reports suggest the uterus is not sterile. Thus, the refluxed menstrual effluent may carry bacteria, and contribute to inflammation, the establishment and growth of endometriotic lesions. Here, we compared and contrasted uterine bacteria (endometrial microbiota) in people with surgically confirmed presence (N = 12) or absence of endometriosis (N = 9) using next-generation 16S rRNA gene sequencing. We obtained an average of > 9000 sequence reads per endometrial biopsy, and found the endometrial microbiota of people with endometriosis was more diverse (greater Shannon Diversity Index and proportion of ‘Other’ taxa) than symptomatic controls (with pelvic pain, surgically confirmed absence of endometriosis; diagnosed with other benign gynecological conditions). The relative abundance of bacterial taxa enriched in the endometrial microbiota of people with endometriosis belonged to the Actinobacteria phylum (Gram-positive),Oxalobacteraceae(Gram-negative) andStreptococcaceae(Gram-positive) families, andTepidimonas(Gram-negative) genus, while those enriched in the symptomatic controls belonged to theBurkholderiaceae(Gram-negative) family, andRalstonia(Gram-negative) genus. Taken together, results suggest the endometrial microbiota is perturbed in people with endometriosis.
Revealing Escherichia coli type II l-asparaginase active site flexible loop in its open, ligand-free conformation
Authors: Maggi Maristella , Meli Massimiliano , Colombo Giorgio , Scotti Claudia 
Published: September 2021

AbstractSince 1993, when the structure of Escherichia coli type II l-asparaginase (EcAII) in complex with l-aspartate was firstly reported, many structures of the wild type and mutated enzyme have been deposited in the Protein Data Bank. None of them report the full structure of the monomer in its ligand-free, open conformation, mainly because of the high dynamic and flexibility of the active site flexible loop. Here we report for the first time the structure of EcAII wild type in its open conformation comprising, for at least one protomer, clear electron density for the active site flexible loop (PDB ID: 6YZI). The structural element is highly mobile and it is transposed onto the rigid part of the active site upon substrate binding to allow completion of the enzyme catalytic center, thanks to key residues that serve as hinges and anchoring points. In the substrate binding pocket, several highly conserved water molecules are coordinated by residues involved in substrate binding, comprising two water molecules very likely involved in the enzyme catalytic process. We also describe, by molecular dynamics simulations, how the transposition of the loop, besides providing the proximity of residues needed for catalysis, causes a general stabilization of the protein.
Computational identification of multiple lysine PTM sites by analyzing the instance hardness and feature importance
Authors: Ahmed Sabit , Rahman Afrida , Hasan Md. Al Mehedi , Ahmad Shamim , Shovan S. M. 
Published: September 2021

AbstractIdentification of post-translational modifications (PTM) is significant in the study of computational proteomics, cell biology, pathogenesis, and drug development due to its role in many bio-molecular mechanisms. Though there are several computational tools to identify individual PTMs, only three predictors have been established to predict multiple PTMs at the same lysine residue. Furthermore, detailed analysis and assessment on dataset balancing and the significance of different feature encoding techniques for a suitable multi-PTM prediction model are still lacking. This study introduces a computational method named ’iMul-kSite’ for predicting acetylation, crotonylation, methylation, succinylation, and glutarylation, from an unrecognized peptide sample with one, multiple, or no modifications. After successfully eliminating the redundant data samples from the majority class by analyzing the hardness of the sequence-coupling information, feature representation has been optimized by adopting the combination of ANOVA F-Test and incremental feature selection approach. The proposed predictor predicts multi-label PTM sites with 92.83% accuracy using the top 100 features. It has also achieved a 93.36% aiming rate and 96.23% coverage rate, which are much better than the existing state-of-the-art predictors on the validation test. This performance indicates that ’iMul-kSite’ can be used as a supportive tool for further K-PTM study. For the convenience of the experimental scientists, ’iMul-kSite’ has been deployed as a user-friendly web-server athttp://103.99.176.239/iMul-kSite.
Unchanged right ventricular strain in repaired tetralogy of Fallot after pulmonary valve replacement with radial long-axis cine magnetic resonance images
Authors: Kawakubo Masateru , Yamasaki Yuzo , Toyomura Daisuke , Yamamura Kenichiro , Sakamoto Ichiro , Moriyama Tetsuhiro , Yabuuchi Hidetake , Ishigami Kousei 
Published: September 2021

AbstractWe measured right ventricular (RV) strain by applying a novel postprocessing technique to conventional short-axis cine magnetic resonance imaging in the repaired tetralogy of Fallot (TOF) and investigated whether pulmonary valve replacement (PVR) changes the RV strain. Twenty-four patients with repaired TOF who underwent PVR and 16 healthy controls were enrolled. Global maximum and minimum principal strains (GPSmax, GPSmin) and global circumferential and longitudinal strains (GCS, GLS) were measured from short-axis cine images reconstructed radially along the long axis. Strain parameters before and after PVR were compared using paired t-tests. One-way ANOVA with Tukey post-hoc analysis was used for comparisons between the before and after PVR groups and the control group. There were no differences in strain parameters before and after PVR. The GPSmax before PVR was lower than that in the control group (P = 0.002). Before and after PVR, GCSs were higher and GLSs were lower than those in the control group (before and after GCSs: P = 0.002 for both, before and after GLSs: P < 0.0001 and P = 0.0003). RV strains from radially reconstructed short-axis cine images revealed unchanged myocardial motion after PVR. When compared to the control group, changes in GCS and GLS in TOF patients before and after PVR might be due to RV remodeling.
Growth hormone promotes hepatic gluconeogenesis by enhancing BTG2–YY1 signaling pathway
Authors: Jo Jeong-Rang , An Seungwon , Ghosh Swati , Nedumaran Balachandar , Kim Yong Deuk 
Published: September 2021

AbstractGrowth hormone (GH) is one of the critical factors in maintaining glucose metabolism. B-cell translocation gene 2 (BTG2) and yin yang 1 (YY1) are key regulators of diverse metabolic processes. In this study, we investigated the link between GH and BTG2–YY1 signaling pathway in glucose metabolism. GH treatment elevated the expression of hepaticBtg2andYy1in primary mouse hepatocytes and mouse livers. Glucose production in primary mouse hepatocytes and serum blood glucose levels were increased during GH exposure. Overexpression of hepaticBtg2andYy1induced key gluconeogenic enzymes phosphoenolpyruvate carboxykinase 1 (PCK1) and glucose-6 phosphatase (G6PC) as well as glucose production in primary mouse hepatocytes, whereas this phenomenon was markedly diminished by knockdown ofBtg2andYy1. Here, we identified the YY1-binding site on thePck1andG6pcgene promoters using reporter assays and point mutation analysis. The regulation of hepatic gluconeogenic genes induced by GH treatment was clearly linked with YY1 recruitment on gluconeogenic gene promoters. Overall, this study demonstrates that BTG2 and YY1 are novel regulators of GH-dependent regulation of hepatic gluconeogenic genes and glucose production. BTG2 and YY1 may be crucial therapeutic targets to intervene in metabolic dysfunction in response to the GH-dependent signaling pathway.