bluuebunny/crossref_metadata_2025_split huggingface dataset:
Platelet-rich plasma treatment improves postoperative recovery in patients with pilonidal sinus disease: a randomized controlled clinical trial
Authors: Boztug Can Yahya , Karaagac Akyol Tulay , Benlice Cigdem , Koc Mehmet Ali , Doganay Erdogan Beyza , Ozcebe Osman Ilhami , Kuzu Mehmet Ayhan , Akyol Cihangir 
Published: October 2021

Abstract
Background
Pilonidal sinus is a common health problem. The current study aimed to compare the impact of autologous platelet-rich plasma (PRP) with that of minimally invasive techniques in terms of pain reduction, return to daily activities, quality of life, and duration of wound healing after open excision and secondary closure.

Methods
Patients who were over 18 years old and had chronic PS disease between March 2018 and January 2019 were enrolled and randomly divided into three groups. Open surgery and moist dressings were applied to patients in group A. Open surgery followed by PRP application was performed on patients in group B. Group C underwent curettage of the sinus cavity followed by application of PRP. In this prospective randomized controlled study, patients completed questionnaires (including the Nottingham Health Profile (NHP), Short Form-36 (SF-36) and clinical information) before and after surgery. Demographics, preoperative characteristics, healing parameters, and quality-of-life scores were evaluated and calculated before and after surgery.

Results and conclusion
The cavity volume and wound-healing time were compared among the groups on postoperative days 0, 2, 3, 4, and 21. Each patient was followed up throughout the process of wound healing, and follow-up was continued afterward to monitor the patients for recurrence. Due to the nature of the treatment that group C received, this group achieved shorter healing times and smaller cavity volume than the other groups. In contrast, the recovery time per unit of cavity volume was significantly faster in group B than in the other groups. Overall postoperative pain scores were significantly lower for both PRP groups (open surgery, group B; minimally invasive surgery, group C) than for group A (p < 0.001) and showed different time courses among the groups. In the treatment of PS disease, PRP application improves postoperative recovery in that it speeds patients’ return to daily activities, reduces their pain scores and increases their quality of life.
Trial registration The current study is registered on the public website ClinicalTrials.gov (ClinicalTrials.gov identifier number: NCT04697082; date: 05/01/2021).
A novel approach of recombinant laterosporulin production using the N-SH2 domain of SHP-2
Authors: Salehzadeh Simin , Tabatabaei Mohammad , Derakhshandeh Abdollah , Karbalaei-Heidari Hamidreza , Kazemipour Nasrin 
Published: October 2021

Abstract
Background
The current study was aimed at evaluating the role of the N-SH2 domain of SHP-2 as a partner protein in the expression of a toxic peptide, laterosporulin (LTS). We also investigated its effects on the formation of the disulfide bond and functional folding of the peptide in vitro. The N-SH2-LTS protein was expressed as a His-tagged fusion protein, capable of undergoing enzymatic cleavage.

Results
Based on the data presented herein, the total yield of the folded fusion protein from inclusion bodies was found to be about 105 mg/l, demonstrating a high-level of heterologous expression. After enzymatic cleavage, 1.5 mg of the folded recombinant laterosporulin was obtained from each 10 mg of the fusion protein. The purity of the recombinant laterosporulin was analyzed by RP-HPLC, to yield peptides with suitable purity (85%).

Conclusions
Our findings indicated the advantages of using the N-SH2 domain of SHP-2 as a rapid and easy approach not only in producing easy target proteins but also in its function as a chaperone. N-SH2 domain of SHP-2 can influence on the purification of laterosporulin at reasonable yield and in a cost-effective fashion. The N-SH2 domain of SHP-2 as a protein chaperone may be potentially favorable to produce other proteins with disulfide bonds.
The antibacterial efficacy of photon-initiated photoacoustic streaming in root canals with different diameters or tapers
Authors: Wen Cheng , Yan Liang , Kong Yuanyuan , Zhao Jian , Li Yang , Jiang Qianzhou 
Published: October 2021

AbstractBackgroundIn recent years, the concept of minimally invasive endodontics has been proposed, which could be described as preventing or treating diseases by preserving more dental tissue and creating minimal damage. In the process of root canal preparation, it was recommended to use instruments with a smaller taper to preserve more tooth tissue and improve the preservation rate of the affected teeth. Photon-induced photoacoustic streaming (PIPS) was a new type of laser-activated irrigation technology, which was now widely used in endodontic treatment. The purpose of this article was to evaluate the bactericidal effect of PIPS with NaOCl in root canals with different widths or tapers.MethodsTwenty-three maxillary first molars with three independent root canals were included in this study. The mesiobuccal (MB), distobuccal (DB), and palatal (P) root canals were prepared at sizes of #10/.02, #25/.02, and #25/.06, respectively. After being incubated with a bacterial suspension for 4 weeks, the specimen were irrigated with 2% NaOCl activated by conventional needle irrigation (CNI) (n = 10) or PIPS (n = 10). Three specimen were not treated (control group). Before and after irrigation, the presence of bacteria was assessed with an adenosine 5'-triphosphate (ATP) assay kit and biofilms were assessed using confocal laser scanning microscopy and scanning electron microscopy.ResultsIn specimen prepared using PIPS irrigation, the ATP was reduced by more than 98%. When the root canal taper was 0.02, the size #25 root canals had a higher percentage of dead bacteria than the size #10 root canals in all regions (P < 0.05) in the PIPS group. When the root canal width was #25, the 0.02 taper group had a higher percentage of dead bacteria than the 0.06 taper group in the apical region (P < 0.05), except coronal and middle regions (P > 0.05). PIPS irrigation results in a greater percentage of dead bacteria and reduction of ATP in size #10/.02 root canals than CNI in size #25/.06 root canals in three regions (P < 0.05).ConclusionIncreasing the width from #10 to #25 improves the bactericidal effect of PIPS in the root canal. Increasing the taper of the root canal from 0.02 to 0.06 at size #25 did not affect the bactericidal effects of PIPS. PIPS resulted in more dead bacteria in specimen with smaller tapers and root canal widths than CNI. PIPS can be used to clean the smear layer in the coronal region and open the dentin tubules.Clinical significance: Activation of irrigants with PIPS brought about significant bacterial reduction smaller tapers and width root canals compared to CNI, which was beneficial to prevent excessive loss of tooth tissue and conserve the structural integrity of teeth.
Clinical and imaging features of carcinosarcoma of the uterus and cervix
Authors: Li Liming , Huang Wenpeng , Xue Kangkang , Feng Leiyu , Han Yijing , Wang Rui , Gao Jianbo 
Published: October 2021

Abstract
Aim
The purpose of our study was to analyze the clinical and imaging features of uterine carcinosarcoma (UCS) and cervical carcinosarcoma (CCS), and to explore the diagnostic and staging accuracy of computed tomography (CT) and magnetic resonance imaging (MRI) examinations.

Methods
41 patients including 37 with UCS and 4 with CCS from July 2011 to September 2020 were enrolled in the study. Of the 37 UCS cases, 7 had CT images, 27 had MRI images, and 3 had both CT and MRI images. The Clinical data, CT or MRI imaging findings were analyzed. Diagnosis and staging accuracy of CT and MRI images were also analyzed.

Results
Carcinosarcoma usually occurs in postmenopausal women (40/41), with the typical clinical symptom being vaginal bleeding (33/41). The CA125 degree was significantly different between the two invasion depth groups (p = 0.011). Most uterine carcinosarcomas showed unclear boundaries, uneven density, low or equal signal on T1WI, high or mixed signal on T2WI, uneven high signal on diffusion-weighted image (DWI), and mild enhancement. The diagnostic accuracies of CT and MRI for carcinosarcoma were 0% and 3.33%, respectively. The diagnostic accuracy for malignant tumors on CT and MRI was 50% and 83.33%, respectively.

Conclusions
Carcinosarcoma lesions presented with huge mass filling in the cavity, and some presented with small polypoid lesions or endometrial thickening. Evaluation of lymph node metastasis is a significant challenge for imaging staging.
Greenhouse gas emissions and carbon sink potential in Eastern Africa rangeland ecosystems: A review
Authors: Mgalula Michael Elias , Wasonga Oliver Vivian , Hülsebusch Christian , Richter Uwe , Hensel Oliver 
Published: October 2021

AbstractMany activities from livestock husbandry contribute to emission and concentration of carbon dioxide (CO2), nitrous oxide (N2O) and methane (CH4) gases to the atmosphere; activities such as grazing, manure and urine deposited or stored on land as well as crop farming practices such as tilling, burning of biomass or crop residues. A better understanding of the extent of emission sources and carbon sequestration potential for Eastern Africa rangelands is vital for developing mitigation strategies. In this article, we review the sources of emission with a focus on land conversion for crop farming, livestock husbandry, wildfire/burning and biotic processes such as soil biota activity in the ecosystem. The trade-offs of using rangeland with an emphasis on enhancing carbon sequestration potential are also addressed. This review revealed that many practices that enhance carbon capture process show promising benefits with sink capacity of −0.004 to 13 Mg C ha−1 year−1. However, given multiple land-use and environmental dynamics in Eastern African rangelands, it is imperative to generate more data across various land management and climatic zones in order to ascertain varied sink capacity. Improving carbon sequestration in rangelands through appropriate land management is a promising cost-effective strategy to mitigate climate change. Through improved farming or grazing management practice and restoring of degraded areas, there are significant benefits to enhance carbon sequestration. As rangeland resources are multi-faceted, engaging trans-disciplinary approaches is necessary to allow analyses of co-benefits of improved management or trade-offs degrading.
Stochastic inversion of magnetotelluric data using deep reinforcement learning
Authors: Wang Han , Liu Yunhe , Yin Changchun , Li Jinfeng , Su Yang , Xiong Bin 
Published: October 2021

We have adopted a new tool to invert magnetotelluric data for the 1D model based on deep Q-networks (DQN), which works as a stochastic optimization method. By transforming the inversion problem into a Markov decision process, the tool learns by trial and error to find the optimal path for updating the model to fit the observed data. The DQN method converges to the target through different paths (e.g., Bayesian or other stochastic methods) and can partially provide the probability distribution of the inversion results, which can be used for uncertainty estimation. The DQN search space gradually decreases as the learning experience progresses, accelerating the single inversion and approximating the optimal result. To check the effectiveness of the DQN inversion, the five- and eight-layer models were separately designed to test the robustness of the DQN for the initial model and the noise level. A further comparison with Occam’s method and the Bayesian method indicated that our DQN obtained more robust inversion results for data contaminated by different noise levels. The inversion results with the survey data from Zhagaitunuoergong area, Inner Mongolia, China, well reveals the shape of the interface basement.
Hospital Volumes of Inpatient Pediatric Surgery in the United States
Authors: Berry Jay G. , Rodean Jonathan , Leahy Izabela , Rangel Shawn , Johnson Connor , Crofton Charis , Staffa Steven J. , Hall Matt , Methot Craig , Desmarais Anna , Ferrari Lynne 
Published: October 2021

BACKGROUND:
Perioperative outcomes of children depend on the skill and expertise in managing pediatric patients, as well as integration of surgical, anesthesiology, and medical teams. We compared the types of pediatric patients and inpatient surgical procedures performed in low- versus higher-volume hospitals throughout the United States.

METHODS:
Retrospective analysis of 323,258 hospitalizations with an operation for children age 0 to 17 years in 2857 hospitals included in the Agency for Healthcare Research and Quality (AHRQ) Kids’ Inpatient Database (KID) 2016. Hospitals were categorized by their volume of annual inpatient surgical procedures. Specific surgeries were distinguished with the AHRQ Clinical Classification System. We assessed complex chronic conditions (CCCs) using Feudtner and Colleagues’ system.

RESULTS:
The median annual volume of pediatric inpatient surgeries across US hospitals was 8 (interquartile range [IQR], 3–29). The median volume of inpatient surgeries for children with a CCC was 4 (IQR, 1–13). Low-volume hospitals performed significantly fewer types of surgeries (median 2 vs 131 types of surgeries in hospitals with 1–24 vs ≥2000 volumes). Appendectomy and fixation of bone fracture were among the most common surgeries in low-volume hospitals. As the volume of surgical procedures increased from 1 to 24 to ≥2000, the percentage of older children ages 11 to 17 years decreased (70.9%–32.0% [P < .001]) and the percentage of children with a CCC increased (11.2%–60.0% [P < .001]).

CONCLUSIONS:
Thousands of US hospitals performed inpatient surgeries on few pediatric patients, including those with CCCs who have the highest risk of perioperative morbidity and mortality. Evaluation of perioperative decision making, workflows, and pediatric clinicians in low- and higher-volume hospitals is warranted.
CT-guided microcoil localization of pulmonary nodules: the effect of the position of microcoil proximal end on the incidence of microcoil dislocation
Authors: Huang Zhen-Guo , Wang Cun-li , Sun Hong-liang , Qin Shu-Zhu , Li Chuan-Dong , Gao Bao-Xiang 
Published: October 2021

Objectives:
To evaluate the effect of the position of microcoil proximal end on the incidence of microcoil dislocation during CT-guided microcoil localization of pulmonary nodules (PNs).

Methods:
This retrospective study included all patients with PNs who received CT-guided microcoil localization before video-assisted thoracoscopic urgery (VATS) resection from June 2016 to December 2019 in our institution. The microcoil distal end was less than 1 cm away from the nodule, and the microcoil proximal end was in the pleural cavity (the pleural cavity group) or chest wall (the chest wall group). The length of microcoil outside the pleura was measured and divided into less than 0.5 cm (group A), 0.5 to 2 cm (group B) and more than 2 cm (group C). Microcoil dislocation was defined as complete retraction into the lung (type I) or complete withdrawal from the lung (type II). The rate of microcoil dislocation between different groups was compared.

Results:
A total of 519 consecutive patients with 571 PNs were included in this study. According to the position of microcoils proximal end on post-marking CT, there were 95 microcoils in the pleural cavity group and 476 in the chest wall group. The number of microcoils in group A, B, and C were 67, 448 and 56, respectively. VATS showed dislocation of 42 microcoils, of which 30 were type II and 12 were type I. There was no statistical difference in the rate of microcoil dislocation between the pleural cavity group and the chest wall group (6.3% vs 7.6%, x2 = 0.18, p = 0.433). The difference in the rate of microcoil dislocation among group A, B, and C was statistically significant (11.9%, 5.8%, and 14.3% for group A, B, and C, respectively, x2 = 7.60, p = 0.008). In group A, 75% (6/8) of dislocations were type I, while all eight dislocations were type II in group C.

Conclusions:
During CT-guided microcoil localization of PNs, placing the microcoil proximal end in the pleura cavity or chest wall had no significant effect on the incidence of microcoil dislocation. The length of microcoil outside the pleura should be 0.5 to 2 cm to reduce the rate of microcoil dislocation.

Advances in knowledge: :
CT-guided microcoil localization can effectively guide VATS to resect invisible and impalpable PNs. Microcoil dislocation is the main cause of localization failure. The length of microcoil outside the pleura is significantly correlated with the rate and type of microcoil dislocation. Placing the microcoil proximal end in the pleura cavity or chest wall has no significant effect on the rate of microcoil dislocation.
Detecting variations in ovulation and menstruation during the COVID-19 pandemic, using real-world mobile app data
Authors: Nguyen Brian T. , Pang Raina D. , Nelson Anita L. , Pearson Jack T. , Benhar Noccioli Eleonora , Reissner Hana R. , Kraker von Schwarzenfeld Anita , Acuna Juan 
Published: October 2021

Background
As war and famine are population level stressors that have been historically linked to menstrual cycle abnormalities, we hypothesized that the COVID-19 pandemic could similarly affect ovulation and menstruation among women.

Methodology
We conducted a retrospective cohort study examining changes in ovulation and menstruation among women using the Natural Cycles mobile tracking app. We compared de-identified cycle data from March-September 2019 (pre-pandemic) versus March-September 2020 (during pandemic) to determine differences in the proportion of users experiencing anovulation, abnormal cycle length, and prolonged menses, as well as population level changes in these parameters, while controlling for user-reported stress during the pandemic.

Findings
We analyzed data from 214,426 cycles from 18,076 app users, primarily from Great Britain (29.3%) and the United States (22.6%). The average user was 33 years of age; most held at least a university degree (79.9%). Nearly half (45.4%) reported more pandemic-related stress. Changes in average cycle and menstruation lengths were not clinically significant, remaining at 29 and 4 days, respectively. Approximately 7.7% and 19.5% of users recorded more anovulatory cycles and abnormal cycle lengths during the pandemic, respectively. Contrary to expectation, 9.6% and 19.6% recorded fewer anovulatory cycles and abnormal cycle lengths, respectively. Women self-reporting more (32.0%) and markedly more (13.6%) stress during the pandemic were not more likely to experience cycle abnormalities.

Conclusions
The COVD-19 pandemic did not induce population-level changes to ovulation and menstruation among women using a mobile app to track menstrual cycles and predict ovulation. While some women experienced abnormalities during the pandemic, this proportion was smaller than that observed prior to the pandemic. As most app users in this study were well-educated women over the age of 30 years, and from high-income countries, their experience of the COVID-19 pandemic might differ in ways that limit the generalizability of these findings.
Study protocol for WHO and UNICEF estimates of global, regional, and national preterm birth rates for 2010 to 2019
Authors: De Costa Ayesha , Moller Ann-Beth , Blencowe Hannah , Johansson Emily White , Hussain-Alkhateeb Laith , Ohuma Eric O. , Okwaraji Yemisrach B. , Cresswell Jennifer , Requejo Jennifer H. , Bahl Rajiv , Oladapo Olufemi T. , Lawn Joy E. , Moran Allisyn C. 
Published: October 2021

Background
Preterm birth is a leading cause of death among children under five years. Previous estimates indicated global preterm birth rate of 10.6% (14.8 million neonates) in 2014. We aim to update preterm birth estimates at global, regional, and national levels for the period 2010 to 2019.

Methods
Preterm birth is defined as a live birth occurring before 37 completed gestational weeks, or <259 days since a woman’s last menstrual period. National administrative data sources for WHO Member States with facility birth rates of ≥80% in the most recent year for which data is available will be searched. Administrative data identified for these countries will be considered if ≥80% of UN estimated live births include gestational age information to define preterm birth. For countries without eligible administrative data, a systematic review of studies will be conducted. Research studies will be eligible if the reported outcome is derived from an observational or intervention study conducted at national or sub-national level in population- or facility-based settings. Risk of bias assessments will focus on gestational age measurement method and coverage, and inclusion of special subgroups in published estimates. Covariates for inclusion will be selected a priori based on a conceptual framework of plausible associations with preterm birth, data availability, and quality of covariate data across many countries and years. Global, regional and national preterm birth rates will be estimated using a Bayesian multilevel-mixed regression model.

Discussion
Accurate measurement of preterm birth is challenging in many countries given incomplete or unavailable data from national administrative sources, compounded by limited gestational age assessment during pregnancy to define preterm birth. Up-to-date modelled estimates will be an important resource to measure the global burden of preterm birth and to inform policies and programs especially in settings with a high burden of neonatal mortality.

Trial registration
PROSPERO registration: CRD42021237861.
Effect of flaxseed on systemic inflammation and oxidative stress in diabetic rats with or without chronic kidney disease
Authors: Al Za’abi Mohammed , Ali Haytham , Ali Badreldin H. 
Published: October 2021

Background
Diabetes mellitus (DM) and chronic kidney disease (CKD) are common causes of morbidity and mortality. Flaxseed contains several bioactive compounds that have been shown to possess anti-inflammatory and antioxidative properties. The aim of the present study was to investigate the possible effect of flaxseed in diabetic rats with adenine–induced CKD.

Methods
Male Wister rats (n = 48) were randomly divided into seven equal groups and treated for 33 consecutive days as follows: G1: control. G2 adenine, G3: streptozotocin (STZ), G4: flaxseed, G5: adenine+flaxseed, G6: STZ+flaxseed, G7: adenine+STZ+flaxseed). DM or CKD were experimentally induced by a single intraperitoneal injection of streptozotocin (STZ) or by adenine via oral gavage, respectively.

Results
Rats fed adenine alone exhibited several changes including decreased body weight, increased food and water intake and urine output, increased urinary albumin/creatinine ratio. They also showed an increase in plasma urea and, creatinine, indoxyl sulfate, neutrophil gelatinase-associated lipocalin and cystatin C, and a decrease in renalase activity. These were associated with significant changes in inflammatory and oxidative biomarkers, e.g., increase in 8-isoprostane, 8 -hydroxy -2-deoxy guanosine and decrease in antioxidant enzymes, as well as increase in interleukins 1β and 6, and NF-κB, and a decrease in interlukin-10. Histopathologically, there was increased tubular necrosis and fibrosis. Concomitant administration of adenine and STZ further worsened the renal damage induced by adenine alone. Flaxseed significantly ameliorated the changes caused by adenine and STZ, given either singly or in combination.

Conclusion
These findings suggest that flaxseed is a potential therapeutic agent in attenuating the progression of CKD in diabetes.