bluuebunny/crossref_metadata_2025_split huggingface dataset:
Telemonitoring Padatan Terlarut pada Filtrasi Air Limbah Laundry Menggunakan Sensor TDS dan Sensor pH untuk Media Budidaya Tanaman Hidroponik Berbasis Mikrokontroler
Authors: Luqyana Nadhea Lulu , Arinie Farida , Muzzakhim Azam 
Published: June 2021

Pada Penelitian ini dirancang alat untuk telemonitoring padatan terlarut pada filtrasi air limbah laundry yang digunakan sebagai media tanam hidroponik, sensor pH, dan sensor TDS untuk mengukur dan mendapatkan data padatan terlarut yang mempengaruhi proses pertumbuhan tanaman, kemudian data-data tersebut akan diproses oleh mikrokontroler Arduino Nano sehingga mendapatkan hasil akhir yang akan disimpan pada database dan dikirimkan secara wireless melalui Wemos D1 sehingga dapat ditampilkan dalam aplikasi android. Penelitian yang sudah dilakukan, didapatkan hasil nilai pengukuran padatan terlarut air limbah laundry sebelum difiltrasi untuk nilai Ph sebesar 6,63 dan kepekatan larutan sebesar 250 ppm. Setelah dilakukan filtrasi sebanyak dua kali didapatkan hasil padatan terlarut untuk Ph yaitu 6,32 dan TDS sebesar 1276 ppm. Berdasarkan penelitian ini hasil telemonitoring rata-rata padatan terlarut air filtrasi limbah laundry setelah digunakan sebagai media tanam hidroponik dengan nilai PH sebesar 6,86 dan TDS sebesar 681 ppm. Karena nilai padatan terlarut media tanam tidak sesuai dengan standar minimum pemanfaatan bidang pertanian hidroponik (PH=6,5;kepekatan larutan=850 ppm), sehingga dapat diambil kesimpulan bahwa penggunaan filtrasi air limbah laundry menyebabkan tanaman tumbuh lambat dan tidak normal (kerdil).
Digital Rock Physics: A Geological Driven Workflow for the Segmentation of Anisotropic Ruhr Sandstone
Authors: Balcewicz Martin , Siegert Mirko , Gurris Marcel , Ruf Matthias , Krach David , Steeb Holger , Saenger Erik H. 
Published: June 2021

Over the last 3 decades, Digital Rock Physics (DRP) has become a complementary part of the characterization of reservoir rocks due to the non-destructive testing character of this technique. The use of high-resolution X-ray Computed Tomography (XRCT) has become widely accepted to create a digital twin of the material under investigation. Compared to other imaging techniques, XRCT technology allows a location-dependent resolution of the individual material particles in volume. However, there are still challenges in assigning physical properties to a particular voxel within the digital twin, due to standard histogram analysis or sub-resolution features in the rock. For this reason, high-resolution image-based data from XRCT, transmitted-light microscope, Scanning Electron Microscope (SEM) as well as geological input properties like geological diagenesis, mineralogical composition, sample’s microfabrics, and estimated sample’s porosity are combined to obtain an optimal spatial segmented image of the studied Ruhr sandstone. Based on a homogeneity test, which corresponds to the evaluation of the gray-scale image histogram, the preferred scan sample sizes in terms of permeability, thermal, and effective elastic rock properties are determined. In addition, these numerically derived property predictions are compared with laboratory measurements to obtain possible upper limits for sample size, segmentation accuracy, and a geometrically calibrated digital twin of the Ruhr sandstone. The comparison corresponding gray-scale image histograms as a function of sample sizes with the corresponding advanced numerical simulations provides a unique workflow for reservoir characterization of the Ruhr sandstone.
Adipose Tissue Epigenetic Profile in Obesity-Related Dysglycemia - A Systematic Review
Authors: Andrade Sara , Morais Tiago , Sandovici Ionel , Seabra Alexandre L. , Constância Miguel , Monteiro Mariana P. 
Published: June 2021

BackgroundObesity is a major risk factor for dysglycemic disorders, including type 2 diabetes (T2D). However, there is wide phenotypic variation in metabolic profiles. Tissue-specific epigenetic modifications could be partially accountable for the observed phenotypic variability.ScopeThe aim of this systematic review was to summarize the available data on epigenetic signatures in human adipose tissue (AT) that characterize overweight or obesity-related insulin resistance (IR) and dysglycemia states and to identify potential underlying mechanisms through the use of unbiased bioinformatics approaches.MethodsOriginal data published in the last decade concerning the comparison of epigenetic marks in human AT of individuals with metabolically unhealthy overweight/obesity (MUHO) versus normal weight individuals or individuals with metabolically healthy overweight/obesity (MHO) was assessed. Furthermore, association of these epigenetic marks with IR/dysglycemic traits, including T2D, was compiled.ResultsWe catalogued more than two thousand differentially methylated regions (DMRs; above the cut-off of 5%) in the AT of individuals with MUHO compared to individuals with MHO. These DNA methylation changes were less likely to occur around the promoter regions and were enriched at loci implicated in intracellular signaling (signal transduction mediated by small GTPases, ERK1/2 signaling and intracellular trafficking). We also identified a network of seven transcription factors that may play an important role in targeting DNA methylation changes to specific genes in the AT of subjects with MUHO, contributing to the pathogeny of obesity-related IR/T2D. Furthermore, we found differentially methylated CpG sites at 8 genes that were present in AT and whole blood, suggesting that DMRs in whole blood could be potentially used as accessible biomarkers of MUHO.ConclusionsThe overall evidence linking epigenetic alterations in key tissues such AT to metabolic complications in human obesity is still very limited, highlighting the need for further studies, particularly those focusing on epigenetic marks other than DNA methylation. Our initial analysis suggests that DNA methylation patterns can potentially discriminate between MUHO from MHO and provide new clues into why some people with obesity are less susceptible to dysglycemia. Identifying AT-specific epigenetic targets could also lead to novel approaches to modify the progression of individuals with obesity towards metabolic disease.Systematic Review RegistrationPROSPERO, identifier CRD42021227237.
Epigenetic Changes in Neonates Born to Mothers With Gestational Diabetes Mellitus May Be Associated With Neonatal Hypoglycaemia
Authors: Kasuga Yoshifumi , Kawai Tomoko , Miyakoshi Kei , Saisho Yoshifumi , Tamagawa Masumi , Hasegawa Keita , Ikenoue Satoru , Ochiai Daigo , Hida Mariko , Tanaka Mamoru , Hata Kenichiro 
Published: June 2021

The detection of epigenetic changes associated with neonatal hypoglycaemia may reveal the pathophysiology and predict the onset of future diseases in offspring. We hypothesized that neonatal hypoglycaemia reflects the in utero environment associated with maternal gestational diabetes mellitus. The aim of this study was to identify epigenetic changes associated with neonatal hypoglycaemia. The association between DNA methylation using Infinium HumanMethylation EPIC BeadChip and neonatal plasma glucose (PG) level at 1 h after birth in 128 offspring born at term to mothers with well-controlled gestational diabetes mellitus was investigated by robust linear regression analysis. Cord blood DNA methylation at 12 CpG sites was significantly associated with PG at 1 h after birth after adding infant sex, delivery method, gestational day, and blood cell compositions as covariates to the regression model. DNA methylation at two CpG sites near an alternative transcription start site of ZNF696 was significantly associated with the PG level at 1 h following birth (false discovery rate-adjusted P &lt; 0.05). Methylation levels at these sites increased as neonatal PG levels at 1 h after birth decreased. In conclusion, gestational diabetes mellitus is associated with DNA methylation changes at the alternative transcription start site of ZNF696 in cord blood cells. This is the first report of DNA methylation changes associated with neonatal PG at 1 h after birth.
Distribution Characteristics and Risk Assessment of Mercury in Sediments From Taihu Lake
Authors: Su Hailei , Shi Di , Yang Jiwei , Tao Yanru , Sun Fuhong , Wei Yuan 
Published: June 2021

Persistent pollutants are easily accumulated in sediments, which can cause toxicity to benthos and secondary pollution to water. Mercury (Hg), as one kind of bioaccumulative and persistent pollutant, could cause great harm to water ecology and health, and has been widely concerned. The distribution characteristics and ecological risk of Hg in sediments from Taihu Lake were studied. In addition, the sediment quality criterion (SQC) of Hg was derived using tissue-based toxicity data combined with the phase-equilibrium partitioning (EqP) approach, and the ecological risk level of Hg in Taihu Lake sediment was evaluated. The results showed that Hg was detected in all the 30 sediment samples, and the concentrations of Hg ranged from 9.7 ng/g to 237.0 ng/g, with an average of 76.3 ng/g. The SQC of Hg was calculated to be 108.9 ng/g, and the ecological risk of Hg in the sediments of Taihu Lake is in the middle level, indicating that Hg pollution needs to be paid more attention. The results provide support for environmental management and risk assessment of Hg.
Synthesis of Monolithic TiO2 Aerogels With Relatively Low Shrinkage and Improved Formability Assisted by CTAB
Authors: Niu Tingting , Zhou Bin , Zhang Zehui , Yang Jianming , Ji Xiujie , Shen Jun , Zhang Zhihua , Du Ai 
Published: June 2021

Monolithic TiO2 aerogels without severe shrink were obtained by the sol-gel method with the addition of the surfactant cetyltrimethylammonium bromide (CTAB) to control the hydrolysis and polycondensation process and acetonitrile solvent as the solvent to improve the crystallinity. After CO2 supercritical drying, the shrinkage ratio of monolithic TiO2 aerogels modified by CTAB decreased by up to ∼26.9%, compared with the pure TiO2 aerogel. Their apparent densities were all lower than 300 g/cm3. X-ray Diffraction (XRD), Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM), Fourier Transform infrared spectroscopy (FTIR) and BET Specific Surface Area Analysis were used to analyze the as-synthesized samples. The results revealed that all the samples were anatase-TiO2 phase with nanoporous network structures. The specific surface areas reached 250.2 m2/g confirmed by the BET (Brunaur–Emmett–Teller method) analysis. However, TiO2 aerogels without the addition of CTAB showed evident agglomeration and collapse of the network in comparison with CTAB-added samples. To further study the structure-property relationship, the photocatalysis performance of as-synthesized and 300°C-calcined aerogels was carried out contrastively. Interestingly, the influences of the CTAB adding amount of as-synthesized and calcined TiO2 aerogels are negative and positive, respectively, which is probably due to the synergistic effect of CTAB hindrance and grain refinement. Potentially, This kind of TiO2 aerogels assisted by CATB with low density, small shrinkage, improved formability, high specific surface area and fine crystalline grain may be applied in various applications, such as electrochemistry, photocatalysis, etc.
A Study on Mesoporous Silica Loaded With Novel Photosensitizers HCE6 and Oxaliplatin for the Treatment of Cholangiocarcinoma
Authors: Zhang Pei-Jian , Liu Meng-Dong , Fan Fang-Yong , Liu Ke-Xia 
Published: June 2021

PurposeCholangiocarcinoma (CCA) is a malignant tumor with a high incidence. The therapeutic effect of conventional chemotherapy and radiotherapy is not obvious. Photodynamic therapy (PDT) is an ideal modality to fight cancer, and the nature of photosensitizer limits its application in clinical therapy. The aim of this study was to explore a novel mode of drug delivery for the intervention of bile duct cancer.MethodsOxaliplatin and photosensitizer HCE6 were loaded with mesoporous silica nanoparticles (MSNs) to synthesize Oxaliplatin/HCE6-MSNs (OH-MSNs); the structure of OH-MSNs was characterized by transmission electron microscopy (TEM) and dynamic light scattering (DLS), the drug release rate was detected by high performance liquid chromatography; the cellular activity, apoptosis level, and the expression levels of intracellular apoptosis and autophagy-related factors of OH-MSNs on cholangiocarcinoma cells were observed by CCK-8, flow cytometry, colony formation assay, and Western blot; the effects of OH-MSNs on cholangioma growth were observed by mouse tumor formation, immunohistochemistry, and tissue Tunel staining.ResultsThe release of OH-MSNs to Oxaliplatin was enhanced under acidic conditions; compared with Oxaliplatin or O-MSNs, OH-MSNs showed more potent killing effects against cholangiocarcinoma cells (P&lt;0.05), and exerted notably inhibitory effects on the activity of cholangiocarcinoma cells (P&lt;0.05), promoted their apoptosis (P&lt;0.05), and greatly facilitated the expression of pro-apoptotic factors and autophagic factors in cholangiocarcinoma cells (P&lt;0.05), and markedly inhibited the expression of anti-apoptotic factors and autophagic inhibitory factors (P&lt;0.05); moreover, OH-MSNs could significantly suppress the growth of mouse cholangiocarcinoma (P&lt;0.05) and induce apoptosis of tumor cells compared with Oxaliplatin or O-MSNs (P&lt;0.05).ConclusionMSNs loading greatly increases the killing effect of Oxaliplatin on cholangiocarcinoma cells and upgrades the autophagic level of cholangiocarcinoma cells, while OH-MSNs synthesized by further loading HCE6 have a more apparent killing effect on cholangiocarcinoma cells.
Long Term Experience in Patients With JIA-Associated Uveitis in a Large Referral Center
Authors: Marelli Luca , Romano Micol , Pontikaki Irene , Gattinara Maurizio Virgilio , Nucci Paolo , Cimaz Rolando , Miserocchi Elisabetta 
Published: June 2021

Objectives: To describe demographic, clinical and therapeutic findings of a large cohort of patients with JIA-associated uveitis in a nationwide referral pediatric rheumatology and uveitis center in Northern Italy.Methods: Retrospective study of 125 patients with JIA-associated uveitis followed from 2009 to 2019. Demographic and rheumatologic features including JIA ILAR classification, age at onset, and laboratory data were recorded. Ocular findings collected were: anatomic location of uveitis, laterality, type, recurrence rate, visual acuity, ocular complications, and local therapy. Systemic therapy with conventional and biologic immunosuppressants, occurrence of adverse events, and duration of treatments were recorded.Results: One hundred and twenty-five patients with JIA-associated uveitis were followed for a meantime of 9.2 (±1.7) years. Oligoarticular JIA was present in 92.8% of patients and anterior uveitis in 96%. The most common ocular complications recorded in our sample were posterior synechiae (37.6%), cataract (20.8%), band keratopathy (19.2%), glaucoma (7.2%), and macular edema (5.6%). Conventional immunosuppressants were used in 75.2% of patients with a mean duration of 9.1 years (±5.4), while biologics were administered in 47.2% of them for a period of 5.4 years. Adverse events (AE) were seen in 23% of patients being treated with Methotrexate, in 10.4% of patients treated with Adalimumab, in 38.5% of patients in therapy with Infliximab, and in 14.3% of patients being treated with Tocilizumab. No AE were reported in patients treated with Golimumab, Certolizumab, Abatacept and Rituximab.Conclusions: An aggressive treatment approach for patients with JIA-associated uveitis ensured a low number of ocular complications with a good safety profile.
Immunotherapies for Anti-N-M-methyl-D-aspartate Receptor Encephalitis: Multicenter Retrospective Pediatric Cohort Study in China
Authors: Guang Shiqi , Ma Jiannan , Ren Xiaotun , Zhou Shuizhen , Yang Jian , Zhang Jianzhao , Cao Xiaoshuang , Zhong Linxiu , Ding Xiao , Wang Xiaosu , Ren Changhong , Zhang Weihua , Zhang Linmei , Zhang Min , Sun Jing , Kessi Miriam , Yin Fei , Peng Jing , Jiang Yuwu 
Published: June 2021

Background: Anti-N-methyl-D-aspartate receptor (NMDAR) encephalitis has been discovered for more than a decade, but the establishment of standardized immunotherapy protocol for pediatric patients still needs more clinical evidence.Methods: A multicenter, retrospective study was conducted on pediatric patients diagnosed with anti-NMDAR encephalitis between November 2011 and December 2018. The clinical records including clinical manifestations, immunotherapy strategies, and outcomes were collected and analyzed.Results: A total of 386 patients were included in our study and the median onset age was 8.00 (IQR 4.83–10.90) years. All patients received first-line immunotherapy and the majority (341, 88.3%) used the standard combination of methylprednisolone pulses (MEP) and intravenous immunoglobulins (IVIG), but 211 patients did not show satisfactory improvement (mRS ≥ 3). Mainly three treatment strategies were applied after first-line immunotherapy: second-line immunotherapy, repetitive first-line immunotherapy, and maintaining oral prednisolone. For patients with mRS ≥ 4 after first-line immunotherapy, the incidence of poor outcome (mRS ≥ 3) in oral prednisolone group was higher than that in other treatment groups (p = 0.039). No difference in complete recovery rate (mRS = 0) was found between patients receiving second-line and repetitive first-line immunotherapy, or patients using long-term and short-term prednisolone. Out of 149 patients who received anti-myelin oligodendrocyte glycoprotein antibody (MOG-Ab) test, 27 (18.12%) were positive. Patients with concomitantly positive MOG-Ab showed milder conditions compared to patients with typical anti-NMDAR encephalitis and were more inclined to relapses. We also identified female, MOG-Ab positive, and not receiving second-line and/or repetitive first-line immunotherapy were risk factors for relapses.Conclusions: For patients with mRS ≥ 4 after first-line immunotherapy and patients with concomitantly positive MOG-Ab, second-line immunotherapy is recommended. When second-line immunotherapy is not applicable, repetitive first-line immunotherapy can be considered as an option. Both second-line and repetitive first-line immunotherapy are beneficial to reduce relapse rate. The duration of sequential oral prednisolone can be shortened after fully evaluating patients' conditions.
Evaluation of a Neonatal Resuscitation Training Programme for Healthcare Professionals in Zanzibar, Tanzania: A Pre-post Intervention Study
Authors: Ding Xiang , Wang Li , Msellem Mwinyi I. , Hu Yaojia , Qiu Jun , Liu Shiying , Zhang Mi , Zhu Lihui , Latour Jos M. 
Published: June 2021

Background: Neonatal mortality rates remain high in Sub-Saharan African countries. Improving the newborn resuscitation skills of healthcare professionals is important in addressing this challenge. The aim of this study was to evaluate a neonatal resuscitation training programme delivered over a two-year period for healthcare professionals in Zanzibar, Tanzania.Methods: A pre- and post-intervention study was designed. We delivered neonatal resuscitation training over a 2-day period in 2017 and 2 days of refresher training in 2018. Knowledge was evaluated by a self-designed survey (11 items with a total score of 22) before and after the two training periods, and skills were evaluated by a skills checklist (six domains with 25 items with a total score of 50) completed by the trainers based on their observations. Statistical analysis included differences in the knowledge and skills scores before and after the training sessions and between the two periods.Results: A total of 23 healthcare professionals participated and completed both neonatal resuscitation training sessions. The knowledge mean scores before and after the training in 2017 increased from 9.60 to 13.60 (95% CI: −5.900; −2.099, p &lt; 0.001), and in 2018, the scores increased from 10.80 to 15.44 (95% CI: −6.062; −3.217, p &lt; 0.001). The mean knowledge scores post-training over time were 13.60 in 2017 and 15.44 in 2018 (95% CI: −3.489; 0.190, p = 0.030). The resuscitation skills performance between the two time periods increased from a mean of 32.26 (SD = 2.35) to a mean of 42.43 (SD = 1.73) (95% CI: −11.402; −8.945, p &lt; 0.001).Conclusion: The neonatal resuscitation training programme increased the theoretical knowledge and resuscitation skills before and after the two training sessions and over time after a 9-month period. Continuous neonatal resuscitation training based on the local needs in resource-limited countries is essential to provide confidence in healthcare professionals to initiate resuscitation and to improve newborn outcomes.
Stable Gastric Pentadecapeptide BPC 157 and Wound Healing
Authors: Seiwerth Sven , Milavic Marija , Vukojevic Jaksa , Gojkovic Slaven , Krezic Ivan , Vuletic Lovorka Batelja , Pavlov Katarina Horvat , Petrovic Andrea , Sikiric Suncana , Vranes Hrvoje , Prtoric Andreja , Zizek Helena , Durasin Tajana , Dobric Ivan , Staresinic Mario , Strbe Sanja , Knezevic Mario , Sola Marija , Kokot Antonio , Sever Marko , Lovric Eva , Skrtic Anita , Blagaic Alenka Boban , Sikiric Predrag 
Published: June 2021

Significance: The antiulcer peptide, stable gastric pentadecapeptide BPC 157 (previously employed in ulcerative colitis and multiple sclerosis trials, no reported toxicity (LD1 not achieved)), is reviewed, focusing on the particular skin wound therapy, incisional/excisional wound, deep burns, diabetic ulcers, and alkali burns, which may be generalized to the other tissues healing.Recent Advances: BPC 157 has practical applicability (given alone, with the same dose range, and same equipotent routes of application, regardless the injury tested).Critical Issues: By simultaneously curing cutaneous and other tissue wounds (colocutaneous, gastrocutaneous, esophagocutaneous, duodenocutaneous, vesicovaginal, and rectovaginal) in rats, the potency of BPC 157 is evident. Healing of the wounds is accomplished by resolution of vessel constriction, the primary platelet plug, the fibrin mesh which acts to stabilize the platelet plug, and resolution of the clot. Thereby, BPC 157 is effective in wound healing much like it is effective in counteracting bleeding disorders, produced by amputation, and/or anticoagulants application. Likewise, BPC 157 may prevent and/or attenuate or eliminate, thus, counteract both arterial and venous thrombosis. Then, confronted with obstructed vessels, there is circumvention of the occlusion, which may be the particular action of BPC 157 in ischemia/reperfusion.Future Directions: BPC 157 rapidly increases various genes expression in rat excision skin wound. This would define the healing in the other tissues, that is, gastrointestinal tract, tendon, ligament, muscle, bone, nerve, spinal cord, cornea (maintained transparency), and blood vessels, seen with BPC 157 therapy.