bluuebunny/crossref_metadata_2025_split huggingface dataset:
An Italian Hotel’s Partnership with the WWF, the World Wildlife and Nature Conservation Charity
Authors: Lods Aurélie , Louis Oriane , Quemener Margot , Sanjivy Agnès 
Published: June 2021

Abstract

Oasy Hotel is set in a protected natural area and welcomes guests looking to reconnect with nature and wide open spaces. The hotel operates according to principles of sustainability, serving organic food from its own farms and offering activities that raise awareness of environmental issues. The hotel is referenced in the Regenerative Travel directory as the first social and ecological sustainable resort in Italy, and the third in Europe. The Covid-19 pandemic has been a challenge but also represents an opportunity, due to a heightened interest in nature-based experiences (
Alina-Cerasela and Daniel, 2020
).

Information
Written on the basis of material provided by Matteo Pennacchi and Andrea Manes of the Oasy Hotel, in the context of Skema’s Strategic Marketing Projects programme.
© CAB International 2021
Analytical Hierarchy Process to Assess the Supply Chain Risk for Improving Sustainability of Shallot Agribusiness in Low Land Area
Authors: Suhaeni  , Andayani Sri Ayu 
Published: June 2021

Abstract
Shallots are one of the potential horticultural commodities, but they face various risks that affect the performance of the supply chain for shallot agribusiness. To reduce potential risks, it is necessary to identify various types of risks with a priority scale. This study is to analyse various risks in supply chain flows and the involvement of various actors in shallot agribusiness in Majalengka Regency in the lowlands. The research was conducted using a descriptive survey method and data collection of 50 respondents through interviews and questionnaires. The collected data were analysed using the Analytical Hierarchy Process (AHP) model. The results showed that the risks that occurred in the lowlands were production risk (15.6%), price risk (14.2%), market risk (10.9%), environmental risk (8.7%), and distribution risk. (8.6%). The actors involved consist of farmers, farmer groups, traders, middlemen, retailers, and consumers. The results of this study can emphasize to facilitate decision making in risk mitigation by paying attention to the priority scale.
Rain Detection using Himawari-8 Imagery; Case Study Singkawang West Kalimantan
Authors: Dharma C S , Trilaksono N J 
Published: June 2021

Abstract
The occurrence of convective activity often appears in a localized area and short duration. The formation of a low cloud might give an insight into the formation of bigger cumulus that can be seen as a Convective Initiation (CI) and if constantly developing it may lead to a bigger cloud with extreme rainfall. Detection of convective activity that leads to extreme rainfall is important to people who live near shore and areas that prone to flood. Himawari-8 imagery and data from an automatic weather station in Singkawang, West Kalimantan, was applied to detect short time, low-intensity rainfall that had been observed in the equator, near shore, during midnight and early morning. The appearance of CI was analyzed using rapid cloud-top cooling based on Himawari-8 imagery. It was found that the observed rainfall was in agreement with the cloud formations. Those data were validated by three days of a rain event with maximum cumulative rain of 30 mm observed in a single day. There was also appeared a small low cloud that grows into a bigger cloud above the observed area.
Characteristics and Depositional Processes of Coastal and Marine Sediments of The Northern Part of Obi Island, Molucca
Authors: Zuraida R , Adhirana I , Lattuputty G , Hernawan U , Gustiantini L , Hasanah I , Alam M , Prasetyo F B 
Published: June 2021

Abstract
This paper presents the characteristics of coastal and marine sediments of the northern part of Obi Island, Molucca. Grain size analyses were conducted on 24 coastal sediment and 32 marine sediment samples. Taphonomy of Homotrema rubrum was studied on three samples from coral reef that grows on the slope off Laiwui to determine current direction. The mean grain size ranges between 0.01 – 2.6 mm, very well sorted to very poorly sorted, and while skewness varies from very finely skewed to very coarsely skewed. While lithic fragments predominate coastal sediments, biogenic fragments form the majority of marine sediment grains. Textural parameters and sediment composition show three distinctive groups that represent: 1) Akeleletango in the west that is characterized by moderately sorted coarse to fine sand; 2) Tabuji – Laiwui that is characterized by poor to moderately sorted fine granules to fine sand that is interpreted as debris flow deposit; and 3) Anggai in the east that is characterized by poorly sorted coarse sand. Linear discriminate function analysis shows the dominance of shallow marine deposits in beach sediments with strong fluvial influence. The taphonomy of H. rubrum indicates that the prevailing current direction off Obi is westward, which is supported by current observation.
Approaching SWOT Analysis to Develop Strategies of Marine-Ecotourism in Bedono Village, Sayung, Demak
Authors: Rezagama A , Setyati W A , Agustini T W , Sunaryo  , Devi S A , Deswanto E , Budiati I M 
Published: June 2021

Abstract
Climate change and abrasion have had a wide impact in various regions of the world. In the area of Central Java Province, such as the coast of Semarang-Demak, there has been a significant change from being an agrarian society to become a coastal area. This raises the potential for coastal disaster-based ecotourism. However, there are many challenges that need to be assessed first to provide a basis for the development direction. Ecotourism provides direct benefits for nature and community conservation in the context of revitalizing resources to rebuild socio-economic conditions that have not been properly managed. The purpose of this study is to examine the development of Disaster-Marine-Ecotourism using a SWOT analysis. The application of SWOT analysis in maritime tourism planning in Bedono village found some specific and unique points. The biggest challenge are developing the competence of local leaders in the tourism sector and improving tourism support infrastructure. Meanwhile, the biggest threat from outside is environmental conditions which are getting worse every year. So synergy from various parties, especially the government, is needed in providing disaster response programs in the area.
Application of Nano Organic Materials in Agriculture farming and yield analysis for Groundnut crop with comparison to conventional inorganic farming
Authors: Patel Anant 
Published: June 2021

Abstract
Agriculture is the primary source of income for the majority of India’s people. Big farmers in India use inorganic fertilisers and pesticides on their plantations. However, it would reduce soil fertility. So, in order to address this problem, this paper encourages farmers to return to organic farming, while still increasing crop yield and decreasing crop water requirements. Nano materials and different bio fertilizers were used to cultivate the groundnut crop. For this, we applied these fertilisers to one acre of land for crop production, whereas on the other hand, we applied simple organic fertilisers and water to another one acre of land for comparison. The key goal is to increase yield with high-quality groundnuts. The use of these Nano organic products results in a 17-20% increase in overall production, and the cost-benefit ratio is 1.98, which is very good as compared to conventional inorganic farming.
Synthesis of Metal Complex for Photocatalytic Degradation of Dyes under Visible Light Irradiation
Authors: Kumar Hemant , Kaur Amandeep 
Published: June 2021

Abstract
In the present work, Schiff base ligand has been synthesized from synthesized dialdehyde and primary amine. The synthesized ligand and its copper metal complex were characterized by melting point & spectral analysis such as IR, 1HNMR, 13C and GC-MS. In the presence of H2O2 as an oxidising agent the photocatalytic performance of the complex was assessed by the photodegradation of dyes. By carrying spectrophotometrically on irradiation of visible light using Cu(II) metal complex of Schiff base ligand the photocatalytic degradation of Naphthol Blue Black dye (NBB), Rhodamine B (RB) and Methylene Blue (MB) dyes was carried out. The photocatalytic degradation of NBB, RB and MB dyes was carried out with reference to effect of time. The results revealed that Cu-complex of Schiff base ligand is consistent for photocatalysis of these dyes for treatment of authentic effluents. The results show that the optimal dose for maximal degradation of these dyes was found to be 5 mgL-1 of the Cu(II) metal complex. The % degradation of NBB, RB and MB were found to be 55%, 50% and 80% in the visible light irradiation. The % degradation of MB dye was greater as compare to other two dyes. The order of % degradation of three dyes by copper metal complex was found as MB > NBB >RB
Social media as tool for the Church’s mission and response to the COVID-19 pandemic
Authors: Galang Justine Renus F , Galang Joseph Renus F 
Published: June 2021

Abstract
A recent correspondence claimed that the establishment of community pantries across the Philippines embodies a spirituality of “Alay Kapwa” as it connects people, especially in a time of crisis such as the 2019 coronavirus (COVID-19) pandemic, to be persons for others. 1 This article highlights that the rise of community pantries was primarily aided by social media. It claims that if social media is utilized ethically and with positive disposition and perspective, it can be a powerful tool in the Church’s mission of evangelization, and in its response to this pandemic.
Fatty acid compartmentalization
Authors: Coleman Rosalind 
Published: June 2021

Before long‐chain fatty acids can be oxidized or used to synthesize complex lipids, they must be activated by one of the acyl‐CoA synthetase (ACSL) family members. Our data in liver, heart, and skeletal muscle has suggested that each activating ACSL isoform can direct fatty acids towards or away from specific downstream pathways. Because we hypothesized that directionality must occur via specific protein interactions, we used the protein interaction discovery technique, BioID, to determine interacting protein partners and function. We targeted ACSL isoform‐1 either to the ER or to the outer mitochondrial membrane (OMM) of Hepa1–6 cells as a fusion protein with the modified E.coli biotin ligase, BirA*. In the presence of added biotin, BirA irreversibly biotinylates proteins that are in close proximity to the ACSL1 bait. Mass spectrometry identified 98 proteins that interacted only with the ER‐targeted ACSL1, 55 proteins that interacted only with the OMM‐targeted ACSL1, and 43 proteins that interacted with both targeted ACSL1 constructs. We selected a subset of lipid droplet and vesicle proteins for confirmation by co‐immunoprecipitation and functional studies. At the OMM, ACSL1 interacted with lipid droplet tethering proteins in a glucose‐dependent manner. At the ER, ACSL1 interacted with proteins involved in ceramide synthesis and with ER and peroxisomal proteins involved in peroxisomal oxidation of niche fatty acids. Thus, we have captured a novel snapshot of key proteins that may influence ACSL1 function and determine downstream partitioning of acyl‐CoAs.Support or Funding InformationDK56598, DK59935This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.
How do enzymes evolve?
Authors: Tawfik Dan S. 
Published: June 2021

I will describe some insights regarding how new proteins functions evolve, via gradual, small changes in sequence. However, the mechanisms of such evolutionary micro‐transitions do not explain macro evolutionary transitions – how completely new proteins emerge, let alone how the very first enzyme(s) evolve. In the second part of my talk I will discuss our recent findings with respect to the de novo emergence of functional, globular proteins.This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.
Functional characterization and design of regulator RNAs using novel high‐throughput tools
Authors: Contreras Lydia M. , Haning Katie , Leistra Abigail N. 
Published: June 2021

Regulatory RNAs enable bacteria to dynamically respond to stresses caused by changes in environmental conditions. Specifically, bacterial small RNAs, a class of RNA regulators, exert dynamic control on complex networks by regulating gene expression. Understanding their functions is a goal in both medicine and metabolic engineering given their relevance to pathogenesis and their potential to manage global regulatory networks that affect biological production of industrially‐relevant compounds. Given the importance of molecular structure to RNA functioning, fundamental sRNA characterization and applied engineering efforts depend heavily on the understanding and design of their specific shapes. Specifically, knowledge of the RNA structural landscape supports identification of interfaces relevant to regulation. In this talk, we will describe the development of a high throughput tool that allows for the simultaneous in vivo characterization of thousands of potential interacting interfaces in RNA molecules, as determined based on their molecular accessibility. We will describe how RNA structural insights obtained from this synthetic probing approach can be used in the functional characterization of newly discovered RNAs and in the rational design of bacterial sRNAs to achieve a tunable gradient of global control for metabolic engineering applications.This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.
Dynamic functional assembly of the Torsin AAA+ ATPase and its modulation by LAP1: a novel mode of regulation for AAA+ ATPases
Authors: Chase A. R. , Laudermilch E. R. , Wang J. , Shigematsu H. , Yokoyama T. R. , Schlieker C. 
Published: June 2021

TorsinA is an essential AAA+ ATPase requiring LAP1 or LULL1 as cofactors. The dynamics of the Torsin/cofactor system remain poorly understood, with previous models invoking Torsin/cofactor assemblies with fixed stoichiometries. Here we demonstrate that TorsinA assembles into homotypic oligomers in the presence of ATP. Torsin variants mutated at the “back” interface disrupt homo‐oligomerization but still show robust ATPase activity in the presence of its cofactors. These Torsin mutants are severely compromised in their ability to rescue nuclear envelope defects in Torsin‐deficient cells, suggesting that TorsinA homo‐oligomers play a key role in vivo. Engagement of the oligomer by LAP1 triggers ATP hydrolysis and rapid complex disassembly. Thus the Torsin complex is a highly dynamic assembly whose oligomeric state is tightly controlled by distinctively localized cellular cofactors. Our discovery that LAP1 serves as a modulator of the oligomeric state of an AAA+ protein establishes a novel means of regulating this important class of oligomeric ATPases. Notably, both the homo‐oligomerization and the ATPase activation are offset by a TorsinA mutation implicated in the movement disorder DYT1 dystonia, possibly contributing to the molecular etiology of this disease.Support or Funding InformationDystonia Medical FoundationNIH NIGMSThis abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.
Caspase‐6 Self‐Activation Enables Distinct Helix‐Strand Interconversion Upon Substrate Binding
Authors: MacPherson Derek J. , Dagbay Kevin B. , Hardy Jeanne A. 
Published: June 2021

Caspases are cysteine proteases that are major players in key cellular processes, including apoptosis and inflammation. Amongst the human caspases, caspase‐6 has been implicated in playing a unique and critical role in the neurodegenerative pathways of Alzheimer's, Huntington's and Parkinson's disease. Unfortunately, structural similarities between caspase‐6 and other caspases have hampered precise targeting of caspase‐6 uniquely. All caspases can exist in a canonical conformation, in which the substrate binds atop a beta‐strand platform in the 130's region. This caspase‐6 region can also adopt a helical conformation that has not been seen in any other caspases. We have shown that caspase‐6 is inherently and dramatically more conformationally dynamic than closely related caspase‐7. In contrast to caspase‐7, which rests constitutively in the strand conformation before and after substrate binding, hydrogen/deuterium exchange data for the L2’ and 130's regions suggested that prior to substrate binding, caspase‐6 exists in a dynamic equilibrium between the helix and strand conformations. Caspase‐6 transitions exclusively to the canonical strand conformation only upon substrate binding. Glu‐135, which showed a noticeably different calculated pKas in the helix and strand conformations, appears to play a key role in the interconversion between the helix and strand conformations. We have also mapped the local changes in the conformational flexibility of procaspase‐6 at the discrete states that reflect series of cleavage events that ultimately lead to the fully active, substrate‐bound state. Intramolecular self‐cleavage at Asp‐193 evoked higher solvent exposure in the regions of the substrate binding loops L1, L3, L4 and in the 130's, the intersubunit linker region, and the 26–32 region, as well as stabilized loop 2. Further removal of the linker allowed caspase‐6 to gain more flexibility in the 130's region and in the L2 region converting caspase‐6 to a competent substrate‐binding state. The prodomain region was found to be intrinsically disordered independent of the activation step of caspase‐6; however, its complete removal resulted in the protection of the adjacent 26–32 region, suggesting that this region may play a regulatory role, likely as an exosite for substrate recognition. The molecular details of caspase‐6 dynamics in solution provide a comprehensive scaffold for strategic design of therapeutic approaches for neurodegenerative disorders.Support or Funding InformationThis work was supported by the National Institutes of Health (NIH) GM 080532.This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.
Biological function of TIMP‐2 is regulated by the composition of the tissue microenvironment
Authors: peeney david , Stetler‐Stevenson William 
Published: June 2021

The activity of mammalian matrix metalloproteinases (MMPs) is regulated by a family of four endogenous inhibitors known as the tissue inhibitor of metalloproteinases (TIMPs). Since their original discovery and characterization several additional physiological functions have been ascribed to TIMP family members, including MMP‐independent biological functions, such as regulation of cell growth and migration. TIMP‐2 is abundantly expressed in normal tissues with low levels of MMP expression and demonstrates broad spectrum inhibition of MMPs, binding to and inhibiting all 23 members of this family and ADAM12 with varying affinities. In addition, TIMP‐2 has been shown to interact with several membrane proteins including MMP14, insulin‐like growth factor‐1 receptor (IGF‐I‐R), low density lipoprotein receptor‐related protein 1 (LRP1) and alpha3 beta1 integrin (a3b1). Recent studies in our lab have shown that TIMP‐2 is a promising candidate for biological cancer therapy. However, the role of TIMP‐2 in tumorigenesis has been subject to debate with varying accounts purporting both anti‐ and pro‐tumor effects. In addition, TIMP‐2 and its putative receptors have been shown to exhibit multiple interactions with components of the tissue microenvironment. Using various cell‐lines we demonstrate that TIMP‐2 cellular binding, signaling and uptake in tumor cells can be differentially regulated by components of the tissue microenvironment resulting in opposing effects on cell behavior, such as intracellular kinase activity in response to growth factors. Our goal is to understand the regulatory factors/conditions that influence the different cellular responses to TIMP‐2 in order to effectively target the aberrant extracellular environment that is associated with many human diseases.This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.
Visualizing translation by ensemble cryo‐EM
Authors: Korostelev Andrei 
Published: June 2021

Translation of mRNA is a key step of gene expression regulation and a therapeutic target. Translation is governed by ribosome dynamics. To visualize the translational machinery in action, we determine high‐resolution ensembles of structures by cryo‐EM. I will discuss how this approach provides insights into the essential aspects of gene expression, such as translation initiation, translation fidelity and ribosome‐induced stress signaling.This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.
New Approaches for Localization of Proteins and Interaction Partners in Cells with High Spatial Resolution
Authors: Carr Steven A. , Udeshi Namrata , Myers Samuel 
Published: June 2021

Labeling of subproteomes within the living cell using genetically‐encoded peroxidase enzyme (APEX; developed in the lab of Alice Ting, Stanford) together with quantitative liquid chromatography‐mass spectrometry is new technology that targets, labels enriches and identifies proteins in subcellular region of interest. In our initial application to the mitochondrial matrix we demonstrated the high specificity of live‐cell peroxidase‐mediated proteomic mapping and the utility for finding novel, previously unknown mitomatrix proteins (Rhee Science 2013). More recently we have extended this powerful new approach to define the molecular composition of unbounded compartments in living cells (Hung Mol Cell 2014; Hung Nature Protocols 2016). Here we illustrate the application of the technology, and recent advances, to define the proteomes of additional sub‐compartments such as excitatory and inhibitory synapses of living neurons. A limitation of the approach has been that few biotinylated sites are identified, reducing the ability to directly obtain information on protein orientation and topology as this requires identifying the sites of biotinylation and not just the proteins that have been modified. This is a known issue with the use of Streptavidin for protein enrichment. We have now developed a new workflow employing anti‐biotin antibodies that increases the numbers of identified sites by >30‐fold and that has manifold potential for studies of biotinylated peptides in a number of other posttranslational modification contexts (Udeshi Nature Methods 2017). Finally, we will describe new work combining recent advances in proximity labeling, genome targeting, and quantitative mass spectrometry to develop genomic locus proteomics (GloPro), a method able to identify proteins that occupy specific genomic loci.Support or Funding InformationThis work is funded by NCI CPTAC PCC 1U24CA210986‐01 to S.A.C. and a Broad Institute SPARC grant to S.M. Portions of this work were also supported by an HHMI Collaborative Innovation Awardsto S.A.C. (Norbert Perrimon PI).This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.