bluuebunny/crossref_metadata_2025_split huggingface dataset:
Generation of liver organoids from human induced pluripotent stem cells as liver fibrosis and steatosis models
Authors: Tsang Hoi Ying , Yi Lo Paulisally Hau , Ho Lee Kenneth Ka 
Published: June 2021

ABSTRACTBackground & AimsLiver cirrhosis is a major cause of death worldwide, and its prevalence is growing rapidly due to the growth of obesity and diabetes population with non-alcoholic fatty liver disease (NAFLD). Yet, no effective therapeutics have been developed to treat NAFLD or its more advanced stage, non-alcoholic steatohepatitis (NASH). This has raised great concern for a representative liver model to be developed so that novel drugs could be screened, identified and developed. Presently, we aim to develop a liver organoid entirely from human induced pluripotent stem cells (hiPSC) to model liver fibrogenesis and NAFLD.MethodsHepatoblasts (HBs), mesenchymal stem cells (MSCs), hepatic stellate cell (HSCs) and endothelial cells (ECs) were derived from hiPSCs, allowed to self-organized and differentiated into liver organoids. Liver functions, transcriptomic and protein expression of liver organoids were characterized and validated. Liver organoids were exposed to thioacetamide (TAA) and free fatty acids (FFA) to be induced into liver disease model.ResultsThe liver organoids we fabricated were highly vascularized, exhibited liver-specific functions and hepatic cellular spatial organization. The presence of liver specific ECs, macrophages and cholangiocytes were found within our organoids. TAA induced fibrosis in our liver organoids that exhibited diminished liver functions, elevated pro-inflammatory cytokines and fibrosis-related gene expression, as well as extensive collagen deposit. Organoids treated with FFA developed steatosis, inflammation and fibrosis.ConclusionsWe generated a novel method, that is Matrigel-independent and size-controllable, for making human liver organoids. These organoids can potentially be utilized as tissue-mimetic in vitro model for high throughput screening to identify drugs that can be used to treat liver fibrosis and NAFLD.
The Lon protease temporally restricts polar cell differentiation events during the Caulobacter cell cycle
Authors: Omnus Deike J. , Fink Matthias J. , Szwedo Klaudia , Jonas Kristina 
Published: June 2021

AbstractThe highly conserved protease Lon has important regulatory and protein quality control functions in cells from the three domains of life. Despite many years of research on Lon, only few specific protein substrates are known in most organisms. Here, we used a quantitative proteomics approach to identify novel substrates of Lon in the dimorphic bacterium Caulobacter crescentus. We focused our study on proteins involved in polar cell differentiation and investigated the developmental regulator StaR and the flagella hook length regulator FliK as specific Lon substrates in detail. We show that Lon recognizes these proteins at their C-termini, and that Lon-dependent degradation ensures their temporally restricted accumulation in the cell cycle phase when their function is needed. Disruption of this precise temporal regulation of StaR and FliK levels in a Δlon mutant contributes to defects in stalk biogenesis and motility, respectively, revealing a critical role of Lon in coordinating developmental processes with cell cycle progression. Our work underscores the importance of Lon in the regulation of complex temporally controlled processes by adjusting the concentrations of critical regulatory proteins. Furthermore, this study includes the first characterization of FliK in C. crescentus and uncovers a dual role of the C-terminal amino acids of FliK in protein function and degradation.
Nascent alt-protein chemoproteomics reveals a repressor of ribosome biogenesis
Authors: Cao Xiongwen , Khitun Alexandra , Harold Cecelia M. , Bryant Carson J. , Zheng Shu-Jian , Baserga Susan J. , Slavoff Sarah A. 
Published: June 2021

AbstractMany unannotated microproteins and alternative proteins (alt-proteins) have recently been found to be co-encoded with canonical proteins, but few of their functions are known. Motivated by the hypothesis that alt-proteins undergoing active or stress-induced synthesis could play important cellular roles, here, we developed a chemoproteomic pipeline to identify nascent alt-proteins in human cells. We identified 22 actively translated unannotated alt-proteins, one of which is upregulated after DNA damage stress. We further defined MINAS-60 (MIcroprotein thatNegatively regulatesASsembly of the pre-60S ribosomal subunit), a nucleolar localized alt-protein co-encoded with human RBM10.Depletion of MINAS-60 increases the amount of the mature 60S ribosomal subunit, consequently upregulating global protein synthesis and cell proliferation by repressing late-stage pre-60S assembly and export of the 60S ribosome subunit to the cytoplasm. Together, these results implicate MINAS-60 as a repressor of ribosome biogenesis, and demonstrate that chemoproteomics can enable generation of functional hypotheses for uncharacterized alt-proteins.
An RNAi screen for genes that affect nuclear morphology in Caenorhabditis elegans reveals the involvement of unexpected processes
Authors: Maheshwari Richa , Rahman Mohammad M. , Joseph-Strauss Daphna , Cohen-Fix Orna 
Published: June 2021

AbstractAberration in nuclear morphology is one of the hallmarks of cellular transformation. However, the processes that, when mis-regulated, result aberrant nuclear morphology are poorly understood. In this study we carried out a systematic, high-throughput RNAi screen for genes that affect nuclear morphology in Caenorhabditis elegans embryos. The screen employed over 1700 RNAi constructs against genes required for embryonic viability. Nuclei of early embryos are typically spherical and their NPCs are evenly distributed. The screen was performed on early embryos expressing a fluorescently tagged component of the nuclear pore complex (NPC), allowing visualization of nuclear shape as well as the distribution of NPCs around the nuclear envelope. Our screen uncovered 182 genes whose down-regulation resulted in one or more abnormal nuclear phenotypes, including multiple nuclei, micronuclei, abnormal nuclear shape, anaphase bridges and abnormal NPC distribution. Many of these genes fall into common functional groups, including some that were not previously known to affect nuclear morphology, such as genes involved in mitochondrial function, the vacuolar ATPase and the CCT chaperonin complex. The results of this screen add to our growing knowledge of processes that affect nuclear morphology and that may be altered in cancer cells that exhibit abnormal nuclear shape.
NON-LINEAR SUSCEPTIBILITY TO INTERFERENCES IN DECLARATIVE MEMORY FORMATION
Authors: Moyano Malen D. , Giulia Carbonari , Matías Bonilla , Pedreira María E. , Brusco Luis I. , Laura Kaczer , Cecilia Forcato 
Published: June 2021

AbstractAfter encoding, memories go through a labile state followed by a stabilization process known as consolidation. Once consolidated they can enter a new labile state after the presentation of a reminder of the original memory, followed by a period of restabilization (reconsolidation). During these periods of lability the memory traces can be modified. Currently, there are studies that show a rapid stabilization after 30 min, while others show that stabilization occurs after longer periods (e.g. 6 h). Here we investigate the effect of an interference treatment on declarative memory consolidation, comparing distinct time intervals after acquisition. On day 1, participants learned a list of non-syllable pairs (List 1). Immediately after, 30 min, 3 h or 8 h later, they received an interference list (List 2) that acted as an amnesic agent. On day 2 (48 h after training) participants had to recall List 1 first, followed by List 2. We found that the List 1 memory was susceptible to interference when the List 2 was administered immediately or 3 h after learning; however, shortly after acquisition (e.g. 30 min) the List 1 memory becomes transiently protected against interference. We propose the possibility that this rapid memory protection could be induced by a fast and transient neocortical integration (where the memory is transiently protected) becoming partially independent from the hippocampus followed by a hippocampal re-engagement where the memory becomes susceptible to interferences again. Our results open a discussion about the contribution of molecular and systemic aspects to memory consolidation.
Road crack detection and quantification based on segmentation network using architecture of matrix
Authors: Li Gang , Chen Yongqiang , Zhou Jian , Zheng Xuan , Li Xue 
Published: June 2021

PurposePeriodic inspection and maintenance are essential for effective pavement preservation. Cracks not only affect the appearance of the road and reduce the levelness, but also shorten the life of road. However, traditional road crack detection methods based on manual investigations and image processing are costly, inefficiency and unreliable. The research aims to replace the traditional road crack detection method and further improve the detection effect.Design/methodology/approachIn this paper, a crack detection method based on matrix network fusing corner-based detection and segmentation network is proposed to effectively identify cracks. The method combines ResNet 152 with matrix network as the backbone network to achieve feature reuse of the crack. The crack region is identified by corners, and segmentation network is constructed to extract the crack. Finally, parameters such as the length and width of the cracks were calculated from the geometric characteristics of the cracks and the relative errors with the actual values were 4.23 and 6.98% respectively.FindingsTo improve the accuracy of crack detection, the model was optimized with the Adam algorithm and mixed with two publicly available datasets for model training and testing and compared with various methods. The results show that the detection performance of our method is better than many excellent algorithms, and the anti-interference ability is strong.Originality/valueThis paper proposed a new type of road crack detection method. The detection effect is better than a variety of detection algorithms and has strong anti-interference ability, which can completely replace traditional crack detection methods and meet engineering needs.
What makes better career opportunities for young graduates? Examining acquired employability skills in higher education institutions
Authors: Ng Peggy M.L. , Chan Jason K. Y. , Wut Tai Ming , Lo Man Fung , Szeto Irene 
Published: June 2021

PurposeThe purpose of this paper is to develop a conceptual model to examine key employability skills that match workplace requirements and foster employability.Design/methodology/approachThis research comprises a cross-sectional study from self-financing institutions in Hong Kong. The current study adopted structural equation modeling to examine key employability skills that match workplace requirements and foster employability.FindingsBased on the empirical findings, the acquired employability skills of young graduates are entrepreneurship, professional development, work with others, self-management, communication and problem solving. Moreover, higher education institutions should work closely with industry stakeholders to get employers engaged with the work-integrating learning (WIL) programs and subsequently equip young graduates for better employability opportunities. In connection with employer engagement, employability skills of communication, problem solving and self-management would be improved. Furthermore, entrepreneurship and problem-solving skills could further be developed for young graduating students working in SME organizations during WIL.Originality/valueAs a notable gap exists in the current literature to examine young graduates' key employability skills in the context and content of Hong Kong self-financing tertiary education, this research explores key employability skills of self-financed young graduates and the relative importance of employability skills across company size using a quantitative approach.
External wine education and certification for restaurant service staff: a mixed-methods evaluation of training effectiveness
Authors: Orlowski Marissa 
Published: June 2021

PurposeThe purpose of this mixed-methods explanatory sequential study was to assess the effects of an external wine education and certification program on trainee reactions, learning, transfer and financial results.Design/methodology/approachThe quantitative phase was a mixed experimental design in which the training intervention was between-subjects and time was within-subjects. The sample comprises 91 employees (NTraining = 43; NControl = 48) from 12 units of a fine dining restaurant group. The qualitative phase, comprised of semi-structured interviews with training group participants (N = 12), was implemented after the experiment.FindingsTraining group participants reported high scores for attitude toward training content, instructional satisfaction and transfer motivation. Financial metrics, tracked up to 60 days post-training, demonstrated the wine education program was effective in increasing wine knowledge but not wine sales. Four themes emerged from the qualitative data: sense of accomplishment, enhanced guest interaction, tips and gratuities and defeat. Integrated findings revealed increased wine knowledge led to personal financial impact (increased tips) rather than organizational impact.Originality/valueThis research builds on existing training literature and human capital theory by examining external training programs. Further, the use of a mixed-methods design and integration of the quantitative and qualitative findings offers a previously unidentified explanation for why wine training, although effective in facilitating positive reactions and learning, fails to result in transfer behaviors which generate increased wine sales.
A missing link: a distributed cognitive perspective on fit
Authors: Herath Gayanga Bandara 
Published: June 2021

PurposeThis article presents a cognitive framework to study dynamic/adaptive aspects of a collection of popular fit measures used in organisation research, in an attempt to highlight what there is to be gained.Design/methodology/approachThis paper uses a distributed e-cognition (DEC) framework to examine the current organisational literature of fit measures.FindingsThis paper highlights that most measures have a rather narrow focus and do not address dynamic/adaptive aspects in complex social systems (e.g. organisations). To both provide a way to integrate fit measures and cover the cognition gap in this literature, this article highlights the need for a more sophisticated measure.Originality/valueThis paper provides a novel approach to examining organisational fit literature through a distributed (e)-cognitive framework.
Impact assessment of innovation tax incentives in Brazil
Authors: Colombo Daniel Gama e , Cruz Helio Nogueira da 
Published: June 2021

PurposeThis paper evaluates the effects of tax incentives on business innovation in Brazil that were established by Law 11,196/05 (the “Fiscal Incentives Law”) to test whether they have had a positive impact on beneficiary firms' innovation input and output and on their performance.Design/methodology/approachThe policy impacts are estimated using microdata on 13,706 firms available in the 2008 and 2011 editions of the Brazilian Innovation Survey (PINTEC) and by applying propensity score matching with difference-in-differences.FindingsThe results suggest a positive and statistically significant impact of the policy on research and development (R&D) expenditures (average of approximately US$ 264,000 in 2011), the number of research staff (average of five researchers) and total employment (approximately 5% of the beneficiary firms' mean size). However, no impact was found on the overall spending on innovative activities, the percentage of sales and exports from new products, net revenue or net revenue per employee.Practical implicationsThe findings provide empirical support in favor of tax incentives as a policy tool to boost business innovation in the country. However, the absence of significant effects on innovative activities expenditures and on most indicators of innovation output and firms' performance reveals shortcomings of the policy that need to be addressed.Originality/valueThe study complements and advances the findings of previous studies by assessing policy impact on total innovative activities expenditures and on innovation output and firm performance.
Influence of trust and risk on peer-to-peer investment willingness: a bidirectional perspective
Authors: Zhai Mengfan , Chen Yuan , Wei Mingxia 
Published: June 2021

PurposeThe purpose of this paper is to investigate the influence of trust and perceived risk on investment willingness considering the bidirectional relationship between trust and perceived risk in peer-to-peer (P2P) lending.Design/methodology/approachData were collected from a leading Chinese P2P platform, PPDAI.com. In total, 328 valid responses were received and analyzed using structural equation modeling (SEM).FindingsThe results show that the influence of trust on investment willingness is significant, whereas that of perceived risk is insignificant. The results also indicate that platform reputation has a positive effect on trust, and the quality of alternatives is positively associated with perceived risk. In addition, the bidirectional perspective should be preferred to cope with the bidirectional relationship between trust and perceived risk in P2P lending.Originality/valueThis study extends existing research on the influence of trust and perceived risk on investment willingness from a bidirectional perspective, which has not been addressed in the P2P lending context. In addition, this research enriches the current literature about trust and perceived risk by providing more evidence that the relationship between trust and perceived risk is bidirectional and thus the bidirectional model should be preferred. For practice, the study suggests that managers can earn trust and reduce the perceived risk of lenders by continuously providing high-quality products, services and enhancing platform reputation, ultimately improving their investment willingness.
A multidisciplinary response to sexual assault: the case review process for previously unsubmitted sexual assault kits
Authors: Melton Heather C. 
Published: June 2021

Purpose
Sexual assault continues to be a major criminal problem. Sexual assault kits (SAK) are one way to preserve evidence to use to pursue justice in sexual assault cases. In recent years, it has become clear that very often these SAKs are never sent to the crime lab to be processed. In an effort to deal with these unsubmitted kits and to research their impact, the Bureau of Justice Assistance funded various grants, known as the Sexual Assault Kit Initiative (SAKI) nationwide to create multidisciplinary teams to both improve the process and response to sexual assault and to provide research on this issue. This paper aims to explore a process created by one of the multidisciplinary teams in one SAKI site – the case review. Ultimately, the goal is to explore how different participants in the case review process perceive and experience the case review and provide implications of these findings.

Design/methodology/approach
Using surveys of case review participants, participant observation and key stakeholder interviews findings indicate that case reviews are beneficial in terms of training, collaboration and overall response to sexual assault.

Findings
Using all methods, the participants of case reviews found them beneficial. Both new information was gleaned from almost every case review and decisions on particular cases were potentially changed, particularly among the key stakeholders with the ability to impact decisions in sexual assault cases – law enforcement and prosecutors. Issues were raised through the case review process that might not have been without this process. Thus, case reviews have the potential to affect policy and practice and improve future reporting, investigations and prosecutions of sexual assault cases.

Practical implications
Multidisciplinary responses to sexual assault cases, specifically the case review process, are beneficial. Issues for training, opportunities for collaboration and general issues for a particular jurisdiction are all potentially raised during a case review. The case reviews need to be organized, preparation work completed and properly facilitated to be effective. Participants in the case review process themselves perceive case reviews to be beneficial.

Originality/value
This paper presents findings from one of the SAKI sites. A specific process, the case review process, that was developed and implemented at this site was explored. The findings on this process have implications for both practice and policy.
Government failures and non-performing loans in European countries: a spatial approach
Authors: Ofria Ferdinando , Mucciardi Massimo 
Published: June 2021

PurposeThe purpose is to analyze the spatially varying impacts of corruption and public debt as % of GDP (proxies of government failures) on non-performing loans (NPLs) in European countries; comparing two periods: one prior to the crisis of 2007 and another one after that. The authors first modeled the NPLs with an ordinary lest square (OLS) regression and found clear evidence of spatial instability in the distribution of the residuals. As a second step, the authors utilized the geographically weighted regression (GWR) to explore regional variations in the relationship between NPLs and the proxies of “Government failures”.Design/methodology/approachThe authors first modeled the NPL with an OLS regression and found clear evidence of spatial instability in the distribution of the residuals. As a second step, the author utilized the Geographically Weighted Regression (GWR) (Fotheringham et al., 2002) to explore regional variations in the relationship between NPLs and proxies of “Government failures” (corruption and public debt as % of GDP).FindingsThe results confirm that corruption and public debt as % of GDP, after the crisis of 2007, have affected significantly on NPLs of the EU countries and the following countries neighboring the EU: Switzerland, Iceland, Norway, Montenegro, and Turkey.Originality/valueIn a spatial prospective, unprecedented in the literature, this research focused on the impact of corruption and public debt as % of GDP on NPLs in European countries. The positive correlation, as expected, between public debt and NPLs highlights that fiscal problems in Eurozone countries have led to an important rise of problem loans. The impact of institutional corruption on NPLs reports that the higher the corruption, the higher is the level of NPLs.