bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification and characterization of hADSC‐derived exosome proteins from different isolation methods
Authors: Huang Lien‐Hung , Rau Cheng‐Shyuan , Wu Shao‐Chun , Wu Yi‐Chan , Wu Chia‐Jung , Tsai Chia‐Wen , Lin Chia‐Wei , Lu Tsu‐Hsiang , Hsieh Ching‐Hua 
Published: July 2021

AbstractExosomes are secreted into the extracellular space by most cell types and contain various molecular constituents, which play roles in many biological processes. Adipose‐derived mesenchymal stem cells (ADSCs) can differentiate into a variety of cell types and secrete a series of paracrine factors through exosomes. ADSC‐derived exosomes have shown diagnostic and therapeutic potential in many clinical diseases. The molecular components are critical for their mechanisms. Several methods have been developed for exosome purification, including ultracentrifugation, ultrafiltration, density gradient purification, size‐based isolation, polymer precipitation and immuno‐affinity purification. Thus, we employed four methods to isolate exosomes from the hADSC culture medium, including ultracentrifugation, size exclusion chromatography, ExoQuick‐TC precipitation and ExoQuick‐TC ULTRA isolation. Following exosome isolation, we performed quantitative proteomic analysis of the exosome proteins using isobaric tags for relative and absolute quantification (iTRAQ) labelling, combined with 2D‐LC‐MS/MS. There were 599 universal and 138 stably expressed proteins in hADSC‐derived exosomes. We proved that these proteins were potential hADSC‐derived exosomes markers, including CD109, CD166, HSPA4, TRAP1, RAB2A, RAB11B and RAB14. From the quantitative proteomic analysis, we demonstrated that hADSC‐derived exosome protein expression varied, with lipopolysaccharide (LPS) treatment, in the different isolation methods. Pathway analysis and proliferation, migration and endothelial tube formation assays showed varying effects in cells stimulated with hADSC‐derived exosomes from different isolation methods. Our study revealed that different isolation methods might introduce variations in the protein composition in exosomes, which reflects their effects on biological function. The pros and cons of these methods are important points to consider for downstream research applications.
Combining multivariate genomic approaches to elucidate the comorbidity between autism spectrum disorder and attention deficit hyperactivity disorder
Authors: Peyre Hugo , Schoeler Tabea , Liu Chaoyu , Williams Camille Michèle , Hoertel Nicolas , Havdahl Alexandra , Pingault Jean‐Baptiste 
Published: July 2021

BackgroundAttention deficit hyperactivity disorder (ADHD) and autism spectrum disorder (ASD) are two highly heritable neurodevelopmental disorders. Several lines of evidence point towards the presence of shared genetic factors underlying ASD and ADHD. We conducted genomic analyses of common risk variants (i.e. single nucleotide polymorphisms, SNPs) shared by ASD and ADHD, and those specific to each disorder.MethodsWith the summary data from two GWAS, one on ASD (N = 46,350) and another on ADHD (N = 55,374) individuals, we used genomic structural equation modelling and colocalization analysis to identify SNPs shared by ASD and ADHD and SNPs specific to each disorder. Functional genomic analyses were then conducted on shared and specific common genetic variants. Finally, we performed a bidirectional Mendelian randomization analysis to test whether the shared genetic risk between ASD and ADHD was interpretable in terms of reciprocal relationships between ASD and ADHD.ResultsWe found that 37.5% of the SNPs associated with ASD (at p < 1e‐6) colocalized with ADHD SNPs and that 19.6% of the SNPs associated with ADHD colocalized with ASD SNPs. We identified genes mapped to SNPs that are specific to ASD or ADHD and that are shared by ASD and ADHD, including two novel genes INSM1 and PAX1. Our bidirectional Mendelian randomization analyses indicated that the risk of ASD was associated with an increased risk of ADHD and vice versa.ConclusionsUsing multivariate genomic analyses, the present study uncovers shared and specific genetic variants associated with ASD and ADHD. Further functional investigation of genes mapped to those shared variants may help identify pathophysiological pathways and new targets for treatment.
Technology‐delivered cognitive‐behavioral therapy for pediatric anxiety disorders: a meta‐analysis of remission, posttreatment anxiety, and functioning
Authors: Cervin Matti , Lundgren Tobias 
Published: July 2021

BackgroundThe efficacy of technology‐delivered cognitive‐behavioral therapy (tCBT) for pediatric anxiety disorders (ADs) is uncertain as no meta‐analysis has examined outcomes in trials that used structured diagnostic assessments at pre‐ and posttreatment.MethodsWe carried out a systematic review and meta‐analysis of randomized controlled trials (RCTs) of tCBT for pediatric ADs that included participants <18 years of age with a confirmed primary AD according to a structured diagnostic interview. Nine studies with 711 participants were included.ResultstCBT outperformed control conditions for remission for primary AD (37.9% vs. 10.2%; k = 9; OR = 4.73; p < .0001; I2 = 0%; moderate certainty), remission for all ADs (19.5% vs. 5.3%; k = 8; OR = 3.32; p < .0001; I2 = 0%; moderate certainty), clinician‐rated functioning (k = 7; MD = −4.38; p < .001; I2 = 56.9%; low certainty), and caregiver‐reported anxiety (k = 7; SMD = 0.27; p = .02; I2 = 41.4%; low certainty), but not for youth‐reported anxiety (k = 9; SMD = 0.13; p = .12; I2 = 0%; low certainty). More severe pretreatment anxiety, a lower proportion of completed sessions, no face‐to‐face sessions, media recruitment, and a larger proportion of females were associated with lower remission rates for primary AD.ConclusionstCBT has a moderate effect on remission for pediatric ADs and clinician‐rated functioning, a small effect on caregiver‐reported anxiety, and no statistically significant effect on youth‐reported anxiety. The certainty of these estimates is low to moderate. Remission rates vary substantially across trials and several factors that may influence remission were identified. Future research should examine for whom tCBT is most appropriate and what care to offer the large proportion that does not remit. Future RCTs should consider contrasting tCBT with partial tCBT (e.g., including therapist‐led exposure) and/or face‐to‐face CBT.
Zebrafish intestinal transcriptome highlights subdued inflammatory responses to dietary soya bean and efficacy of yeast β‐glucan
Authors: Rehman Saima , Gora Adnan H. , Siriyappagouder Prabhugouda , Brugman Sylvia , Fernandes Jorge M. O. , Dias Jorge , Kiron Viswanath 
Published: July 2021

AbstractAnti‐nutritional factors in dietary components can have a negative impact on the intestinal barrier. Here, we present soya bean‐induced changes in the intestine of juvenile zebrafish and the effect of yeast β‐glucan through a transcriptomic approach. The inclusion of soya bean meal affected the expression of several intestinal barrier function‐related genes like arl4ca, rab25b, rhoub, muc5ac, muc5d, clcn2c and cltb in zebrafish. Several metabolic genes like cyp2x10.2, cyp2aa2, aldh3a2b, crata, elovl4, elovl6, slc51a, gpat2 and ATP‐dependent peptidase activity (lonrf, clpxb) were altered in the intestinal tissue. The expression of immune‐related genes like nlrc3, nlrp12, gimap8, prdm1 and tph1a, and genes related to cell cycle, DNA damage and DNA repair (e.g. spo11, rad21l1, nabp1b, spata22, tdrd9) were also affected in the soya bean fed group. Furthermore, our study suggests the plausible effect of yeast β‐glucan through the modulation of several genes that regulate immune responses and barrier integrity. Our findings indicate a subdued inflammation in juvenile zebrafish fed soya bean meal and the efficacy of β‐glucan to counter these subtle inflammatory responses.
Baricitinib reduces 30‐day mortality in older adults with moderate‐to‐severe COVID‐19 pneumonia
Authors: Abizanda Pedro , Calbo Mayo Juan María , Mas Romero Marta , Cortés Zamora Elisa Belén , Tabernero Sahuquillo María Teresa , Romero Rizos Luis , Sánchez‐Jurado Pedro Manuel , Sánchez‐Nievas Ginés , Campayo Escolano Carlos , Ochoa Serrano Alba , Sánchez‐Flor Alfaro Victoria , López Bru Rita , Gómez Ballesteros Cristina , Caldevilla Bernardo David , Callejas González Francisco Javier , Andrés‐Pretel Fernando , Lauschke Volker Martin , Stebbing Justin 
Published: July 2021

AbstractBackgroundOlder adults are at the highest risk of severe disease and death due to COVID‐19. Randomized data have shown that baricitinib improves outcomes in these patients, but focused stratified analyses of geriatric cohorts are lacking. Our objective was to analyze the efficacy of baricitinib in older adults with COVID‐19 moderate‐to‐severe pneumonia.MethodsThis is a propensity score [PS]‐matched retrospective cohort study. Patients from the COVID‐AGE and Alba‐Score cohorts, hospitalized for moderate‐to‐severe COVID‐19 pneumonia, were categorized in two age brackets of age <70 years old (86 with baricitinib and 86 PS‐matched controls) or ≥70 years old (78 on baricitinib and 78 PS‐matched controls). Thirty‐day mortality rates were analyzed with Kaplan–Meier and Cox proportional hazard models.ResultsMean age was 79.1 for those ≥70 years and 58.9 for those <70. Exactly 29.6% were female. Treatment with baricitinib resulted in a significant reduction in death from any cause by 48% in patients aged 70 or older, an 18.5% reduction in 30‐day absolute mortality risk (n/N: 16/78 [20.5%] baricitinib, 30/78 [38.5%] in PS‐matched controls, p < 0.001) and a lower 30‐day adjusted fatality rate (HR 0.21; 95% CI 0.09–0.47; p < 0.001). Beneficial effects on mortality were also observed in the age group <70 (8.1% reduction in 30‐day absolute mortality risk; HR 0.14; 95% CI 0.03–0.64; p = 0.011).ConclusionsBaricitinib is associated with an absolute mortality risk reduction of 18.5% in adults older than 70 years hospitalized with COVID‐19 pneumonia.
Marrying into it: Siblings‐in‐law in the social support networks of adults with intellectual and developmental disabilities
Authors: Bumble J. L. , Sanderson K. A. , Zemke K. E. , Hodapp R. M. 
Published: July 2021

AbstractBackgroundAlthough attention has been devoted to social supports provided by parents and siblings of adults with intellectual and developmental disabilities (IDD), much less is known about supports provided by the spouses of these siblings—the so‐called siblings‐in‐law. This study examined the role of the sibling‐in‐law in the social support networks of their brother/sister‐in‐law with IDD and factors associated with providing greater amounts of tangible, informational and emotional support.MethodSiblings‐in‐law of adults with IDD living predominately in the United States (N = 99) participated in a cross‐sectional survey. Measures covered demographic and family background, perspectives towards having a brother/sister‐in‐law with IDD, supports provided and characteristics of the adult with IDD's social support network.ResultsMost siblings‐in‐law reported providing support to their brother/sister‐in‐law with IDD. Percentages of siblings‐in‐law providing support were similar to that of the spouse and the spouse's family in the area of emotional support and similar to percentages of paid professionals in tangible and informational supports. Across all three support types, the main predictor of increased sibling‐in‐law support levels was a decreased ability of the parents‐in‐law to care for their child with IDD. Other predictors (e.g. distance from brother/sister‐in‐law with IDD and disability ‘insider’ status) varied by support type.ConclusionsFindings highlight the role of siblings‐in‐law in the social support networks of adults with IDD, with implications for policy and practice.
Continuous interprofessional collaboration for women with gestational diabetes mellitus: A cross‐sectional survey in Japan
Authors: Matsunaga Mayumi , Horiuchi Shigeko , Kataoka Yaeko , Igarashi Yumiko , Porter Sarah E. , Fukui Toshiko 
Published: July 2021

AbstractAimThis study aimed to identify the current situation of interprofessional collaboration for gestational diabetes mellitus (GDM) management in Japan including the professionals involved, the collaboration methods employed, and the barriers perceived by nurses and midwives.MethodsThis nationwide cross‐sectional survey of 1,046 total hospitals facilitating childbirth in Japan used an original 60‐item questionnaire to investigate GDM management practice through interprofessional collaboration. The questionnaire required one responder to be a midwife or nurse who was familiar with the management practices for GDM women in their respective hospitals. Quantitative data were analyzed using descriptive statistics, and framework analysis was conducted for qualitative data collected by open‐ended questions.ResultsAll 308 respondents (response rate 29.4%) were included. The professionals included in interprofessional collaboration were limited, and the only strategy used for interprofessional collaboration by a majority (91.5%) of hospitals was medical charts. There were 50.8% of hospitals that provided postpartum GDM follow‐ups and 54.5% of hospitals that did not engage in external collaboration for GDM management. The barriers to interprofessional collaboration extracted were seven categories and 23 subcategories, which were aligned within the following units of analysis: individual, team, organization, and community.ConclusionsThis survey shows that interprofessional collaboration, including support for GDM postpartum follow‐up in Japan remains insufficient. Furthermore, nurses and midwives perceive numerous barriers to interprofessional collaboration for continuous GDM management at the individual, team, organizational and community levels.
Energy‐related financial literacy and electricity consumption: Survey‐based evidence from Finland
Authors: Kalmi Panu , Trotta Gianluca , Kažukauskas Andrius 
Published: July 2021

AbstractWe provide new insights into (i) the level of energy‐related financial literacy in Finnish households, (ii) the sociodemographic characteristics that affect energy‐related financial literacy, and (iii) whether energy‐related financial literacy influences household electricity consumption. We use a data set consisting of energy‐related financial literacy questions combined with monthly electricity consumption data. We draw from the recent literature on energy‐related financial literacy and suggest two new variables based on subjective perceptions on energy‐related financial literacy. Gender has a strong association with energy‐related financial literacy. Our results also indicate that households of respondents with higher levels of energy‐related financial literacy tend to consume less electricity when we control for other factors such as dwelling and household characteristics. This implies that measures to promote energy‐related financial literacy might guide consumers' decisions toward energy efficiency and conservation.
Formyl peptide receptor‐2 is upregulated in the blood and placenta of patients with gestational diabetes mellitus
Authors: Ye Wenfeng , Chen Linlin , Yang Yanjun , Yao Changfang , Zhu Lihua , Wang Qi , Jiang Jingting 
Published: July 2021

AbstractAimTo investigate the expression of formyl peptide receptor 2 (FPR2) in maternal blood, umbilical blood, and placenta of patients with gestational diabetes mellitus (GDM), and to analyze the changes of other pro‐inflammatory cytokines in blood, including interleukin 33 (IL‐33), IL‐1β, tumor necrosis factor alpha (TNF‐α), and C‐reactive protein (CRP), so as to reveal the pathogenesis of GDM.MethodsFPR2, IL‐33, IL‐1β, T TNF‐α, and CRP in maternal blood and umbilical cord blood of 50 pregnant women with GDM and 30 normal pregnant women were analyzed by ELISA method to explore the correlation between inflammatory factors and blood glucose. The expression of FPR2 in placental tissues was analyzed by PCR and immunohistochemistry.ResultsThe expression of FPR2 in maternal blood of gestational diabetes patients was significantly higher than that of normal pregnant women, and other inflammatory factors IL‐33 and IL‐1β in maternal blood were also significantly increased. The expression of FPR2 in umbilical cord blood of gestational diabetes was higher than that of normal pregnant women, but the difference was not significant. Other inflammatory factors IL‐33, IL‐1β, and CRP in umbilical cord blood were also significantly increased. The expression of FPR2mRNA and protein in placental tissues of gestational diabetes was significantly higher than that of normal pregnant women.ConclusionsThe level of FPR2, IL‐33, and IL‐1β in maternal blood was related to the pathogenesis of GDM and these inflammatory factors could be used as special candidate direction of marks for the prevention, clinical treatment and drug design of GDM, laying a new theoretical foundation for the treatment of GDM.
Immunogenicity of SARS‐CoV‐2 mRNA vaccine in solid organ transplant recipients
Authors: Holden Inge K. , Bistrup Claus , Nilsson Anna Christine , Hansen Janne Fuglsang , Abazi Rozeta , Davidsen Jesper Rømhild , Poulsen Mikael Kjær , Lindvig Susan Olaf , Justesen Ulrik S. , Johansen Isik Somuncu 
Published: July 2021

AbstractBackgroundIt is currently not well described if a two‐dose regimen of a Covid‐19 vaccine is sufficient to elicit an immune response in solid organ transplant (SOT) recipients.ResultsA total of 80 SOT recipients completed a two‐dose regimen with severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) messenger RNA vaccine. Only 35.0% (n = 28) were able to mount a positive IgG immune response 6 weeks after the second dose of vaccine.ConclusionThis emphasizes that SOT recipients need continued use of personal protective measures. Future studies need to closely examine the cellular immune response in patients with compromised antibody response to Covid‐19 vaccination.
Teachers' Perceptions and Attitudes Toward Breakfast in the Classroom: The Importance of Health
Authors: McKeon Gina Pope , Shukaitis Jennifer , Cuite Cara L. 
Published: July 2021

ABSTRACTBACKGROUNDTeacher resistance is sometimes cited as a barrier to implementing Breakfast in the Classroom (BIC), an approach to school breakfast in which students eat at their desks after the school day has begun. Given their role in serving breakfast with BIC, teacher perceptions may be critical in implementing an effective BIC program.METHODSK‐5 teachers (N = 249) in an urban, low‐income school district completed an online survey that assessed their expectations of BIC (retrospectively), perceptions of its benefits and challenges, and current program satisfaction. Descriptive statistics, Pearson's correlation tests, and multiple regression analyses were performed.RESULTSMost teachers (64%) whose students were currently receiving BIC were satisfied with the program overall, and 81% of teachers agreed that (1) BIC should continue and (2) is worth the effort. The most commonly identified problem was lack of breakfast variety and healthfulness. Retrospective positive expectations were positively correlated with current program satisfaction (r = .61, p < .001), although overall post‐implementation perceptions are much more positive. Additionally, the perception that BIC improves students' health is related to higher satisfaction with BIC.CONCLUSIONSFocusing on the health implications of BIC may be important to implementing new BIC programs.
Recess Quality and Social and Behavioral Health in Elementary School Students
Authors: Massey William V. , Thalken Janelle , Szarabajko Alexandra , Neilson Laura , Geldhof John 
Published: July 2021

ABSTRACTBACKGROUNDA majority of research findings have focused on recess as instrumental to achieving minutes of physical activity rather than focusing on the psycho‐social benefits associated with a high‐quality recess environment. The purpose of the current study was to examine the relationship between recess quality and teacher‐reported social, emotional, and behavioral outcomes in children.METHODSData were collected from 26 schools in 4 different regions of the United States. Teachers (N = 113) completed behavioral assessments for randomly selected children in their classrooms (N = 352). Data assessors conducted live observations of recess using the Great Recess Framework—Observational Tool. A series of 2‐level regression models were fit in Mplus v. 8.2 to assess how recess quality was associated with indicators of children's social, emotional, and behavioral health.RESULTSRecess quality significantly predicted executive functioning problems (b = −.360, p = .021), resilience (b = .369, p = .016), emotional self‐control (b = −.367, p = .016), and a composite of adaptive classroom behaviors (b = .321, p = .030).CONCLUSIONResults of the present study demonstrate that recess quality impacts child developmental outcomes. Schools should ensure there is adequate training and resources to facilitate a positive and meaningful recess for students.
A JOURNEY THROUGH PYSCHOSIS: MY STORY Learning from lived experience of mental illness
Authors: Thomas Esther Jayne 
Published: July 2021

AbstractMy submission is under the “lived experience narrative” you are kindly publishing. It is the story of my journey through psychosis and aims to elicit empathy from professionals and lay readers alike. It shines a light on the general nature of mental illness hoping to provide an insight to those working in the fields of psychiatry and nursing. At the same time, it touches upon the use of labels, alternative mental health practices and the role of capitalism in mental illness. Ultimately, it shows there is a life to be had when you learn to carry your diagnosis with hope.
Effects of increasing dietary rye levels on physicochemical characteristics of digesta and its impact on stomach emptying as well as the formation of ‘doughballs’ in stomachs of young pigs
Authors: Wilke Volker , Grone Richard , von Felde Andreas , Abd El‐Wahab Amr , Wolf Petra , Kamphues Josef 
Published: July 2021

AbstractDespite quite similar contents of starch and crude fibre of wheat and rye, the unique non‐starch‐polysaccharide fraction of rye (e.g. high levels of arabinoxylans and fructans) might have an impact on physicochemical properties of the digesta in pigs. Forty pigs (age: 46.8 ± 5.28 days; bodyweight: 16.1 ± 4.13 kg) were divided into four treatment groups. During four weeks, the pigs received diets consisting of wheat and/or rye, barley, soy, potato protein and a mineral supplement. The sum of wheat and rye was 69% in all diets, whereby the compound feed of each group was characterized by a different ratio (%) of wheat/rye (69/0; 46/23; 23/46; 0/69, respectively). In the stomach, ‘doughballs’ occurred more frequently with increasing dietary rye levels (9/10; 69% rye). With higher DM content and extract‐viscosity of gastric digesta, the stomach emptying tended to be retarded in rye groups. Compared to the control group (69% wheat), maximum dietary rye levels (69%) resulted in significantly higher concentrations of lactic acid in digesta of the stomach and small intestine. With increasing lactic acid concentrations, the pH tended to be lower in small intestinal digesta. With an intensified formation of lactic acid, effects against Gram‐negative bacteria, for example Salmonella, can be expected. Moreover, because of higher viscosity and the retarded stomach emptying, there could be advantages of including rye in compound feeds when a longer lasting satiety is intended, for example when feeding pregnant sows (regularly fed restrictively).