bluuebunny/crossref_metadata_2025_split huggingface dataset:
Fine mapping of the BnaC04.BIL1 gene controlling plant height in Brassica napus L
Authors: Yang Mao , He Jianbo , Wan Shubei , Li Weiyan , Chen Wenjing , Wang Yangming , Jiang Xiaomei , Cheng Pengfei , Chu Pu , Shen Wenbiao , Guan Rongzhan 
Published: August 2021

AbstractBackgroundPlant height is an important architecture trait which is a fundamental yield-determining trait in crops. Variety with dwarf or semi-dwarf phenotype is a major objective in the breeding because dwarfing architecture can help to increase harvest index, increase planting density, enhance lodging resistance, and thus be suitable for mechanization harvest. Although some germplasm or genes associated with dwarfing plant type have been carried out. The molecular mechanisms underlying dwarfism in oilseed rape (Brassica napusL.) are poorly understood, restricting the progress of breeding dwarf varieties in this species. Here, we report a new dwarf mutantBndwarf2from ourB. napusgermplasm. We studied its inheritance and mapped the dwarf locusBnDWARF2.ResultsThe inheritance analysis showed that the dwarfism phenotype was controlled by one semi-dominant gene, which was mapped in an interval of 787.88 kb on the C04 chromosome ofB. napusby Illumina Brassica 60 K Bead Chip Array. To fine-mapBnDWARF2, 318 simple sequence repeat (SSR) primers were designed to uniformly cover the mapping interval. Among them, 15 polymorphic primers that narrowed down theBnDWARF2locus to 34.62 kb were detected using a F2:3family population with 889 individuals. Protein sequence analysis showed that only BnaC04.BIL1 (BnaC04g41660D) had two amino acid residues substitutions (Thr187Ser and Gln399His) between ZS11 andBndwarf2, which encoding a GLYCOGEN SYNTHASE KINASE 3 (GSK3-like). The quantitative real-time PCR (qRT-PCR) analysis showed that theBnaC04.BIL1gene expressed in all tissues of oilseed rape. Subcellular localization experiment showed that BnaC04.BIL1 was localized in the nucleus in tobacco leaf cells. Genetic transformation experiments confirmed that theBnaC04.BIL1is responsible for the plant dwarf phenotype in theBndwarf2mutants. Overexpression ofBnaC04.BIL1reduced plant height, but also resulted in compact plant architecture.ConclusionsA dominant dwarfing gene,BnaC04.BIL1, encodes an GSK3-like that negatively regulates plant height, was mapped and isolated. Our identification of a distinct gene locus may help to improve lodging resistance in oilseed rape.
The effect of marital and insurance status on the survival of elderly patients with stage M1b colon cancer: a SEER-based study
Authors: Zhou Chenghui , Zhang Yiwei , Hu Xi , Fang Min , Xiao Shuai 
Published: August 2021

Abstract
Background
Colon cancer is largely implicated in elderly patients (age ≥ 60 years). The prognosis of patients diagnosed with the M1b stage is vastly poor. Marital and insurance status has been considered important prognostic factors in various cancer types. However, how these factors influence elderly patients with stage M1b colon cancer remains to be explored. This study aims to uncover the role of marital and insurance status in the survival of elderly patients with stage M1b colon cancer.

Methods
We retrieved data for patients diagnosed with stage M1b colon cancer between 2010 and 2016 from the Surveillance, Epidemiology, and End Results (SEER) database. Our analysis of the clinicopathological features, overall survival (OS), and cancer-specific survival (CSS) was based on the marital and insurance status, respectively.

Results
In sum, 5709 stage M1b colon cancer patients with complete information from SEER were enrolled for analysis. The OS and CSS of the Non-married group were poorer compared to that of the Married group. The OS and CSS of the Uninsured group were poorer than both of the Insured group and Medicaid group. However, OS was comparable between Uninsured group and Medicaid groups. The findings allude that marital and insurance status potentially impact the long-term survival of elderly patients with M1b colon cancer. The subgroup survival analyses revealed the lowest risk for death among the Insured Married group based on the comparison of the OS and CSS across all other groups. Moreover, Univariate and multivariate analyses revealed race, marital status, surgery, and chemotherapy as independent predictors for OS, whereas insurance status, surgery,and chemotherapy were independent predictors for CSS in elderly patients with M1b colon cancer.

Conclusion
The marital and insurance status greatly impact the survival of elderly patients with M1b colon cancer. Therefore, it is imperative to provide more support to this vulnerable patient group who are lonely and uninsured, particularly in the psychological and health insurance aspect.
Experiences of using a digital type 2 diabetes prevention application designed to support women with previous gestational diabetes
Authors: Ekezie Winifred , Dallosso Helen , Saravanan Ponnusamy , Khunti Kamlesh , Hadjiconstantinou Michelle 
Published: August 2021

Abstract
Background
Gestational diabetes mellitus (GDM) is diagnosed during pregnancy, and women with a history of GDM are at a higher risk of developing type 2 diabetes mellitus (T2DM). Prevention strategies focused on lifestyle modification help to reduce long-term complications. Self-management technology-based interventions can support behaviour change and diabetes control. The Baby Steps programme, a randomised controlled trial intervention offering group education and access to a mobile web application, was evaluated to explore user experience of the app and barriers and facilitators to app usability.

Methods
Ten semi-structured interviews and four focus group discussions were conducted with 23 trial participants between 2018 and 2019. Interviews and focus group discussions were audiotaped, transcribed and independently analysed. The analysis was informed by thematic analysis, with the use of the Nvivo 12 software.

Results
Themes identified were: (1) GDM and post-pregnancy support from healthcare services; (2) Impact of Baby Steps app on lifestyle changes; (3) Facilitators and barriers to the usability of the Baby Steps app. The Baby Steps app served as a motivator for increasing self-management activities and a tool for monitoring progress. Peer support and increased awareness of GDM and T2DM enhanced engagement with the app, while poor awareness of all the components of the app and low technical skills contributed to low usability.

Conclusions
This study documents experiences from existing GDM support, user experiences from using the Baby Steps app, and the barriers and facilitators to app usability. The app was both a motivational and a monitoring tool for GDM self-management and T2DM prevention. Peer support was a key trait for enhanced engagement, while barriers were low technical skills and poor awareness of the app components. A digital app, such as the Baby Steps app, could strengthen existing face-to-face support for the prevention of T2DM. The results also have wider implications for digital support technologies for all self-management interventions. Further research on the effect of specific components of apps will be required to better understand the long term impact of apps and digital interventions on self-management behaviours and outcomes.

Trial registration
ISRCTN, ISRCTN17299860. Registered on 5 April 2017.
Axillary Paget disease with a visible satellite: a case report and literature review
Authors: Ji Wu-Yang , Luo Bin , Wang Xue-Wei , Xiao Ying , Tian Jin-Yi 
Published: August 2021

Abstract
Background
Extramammary Paget disease (EMPD) is an uncommon malignancy affecting apocrine gland–bearing skin, such as vulvar, perianal, axillary and penoscrotal areas. Paget cells are sometimes detected outside clinical border in a phenomenon called subclinical extension. Satellite is one of the patterns of subclinical extension which is likely to be invisible. The standard management strategy for EMPD without distant metastasis is a complete surgical removal, sometimes called wide local excision. However, there is no consensus regarding surgical margin width to decrease the high recurrence rate. Here we describe the first macroscopically visible satellite of axillary EMPD and wide local excision of both main lesion and the satellite lesion with only 0.5 cm margin, succeeded by a short review of the literature.

Case presentation
A 48-year-old female with a red macule in the right axilla was presented to our clinic. A well-demarcated 4 cm × 3 cm erythematous plaque was observed in the right axilla, and a similar lesion measuring 0.5 cm × 0.3 cm was found 3.5 cm away from the primary site. Breast and axillary node examination was unremarkable. Biopsy of the large plaque revealed Paget disease, then we performed a local extended excision of both lesions with a 0.5 cm margin, all margins negative indicated, by frozen pathology. Pathology revealed the nature of the satellite beside the main lesion also as Paget disease. The patient is currently followed-up for 20 months and has shown no signs of recurrence, with normal shoulder motor function.

Conclusion
We have report the first visible satellite of extramammary Paget disease, indicating the necessity of an extended local resection of both the main leision and the satellite lesion. Considering the anatomical structure of axillary Paget disease, a 0.5 cm negative surgical margin indicated by frozen pathology might be sufficient to sustain the shoulder motor function.
IL-1β primed mesenchymal stromal cells moderate hemorrhagic shock-induced organ injuries
Authors: Aussel Clotilde , Baudry Nathalie , Grosbot Marion , Caron Cécile , Vicaut Eric , Banzet Sébastien , Peltzer Juliette 
Published: August 2021

Abstract
Background
Organ damages following hemorrhagic shock (HS) have been partly attributed to an immunological dysfunction. The current challenge in the management of HS patients is to prevent organ injury-induced morbidity and mortality which currently has not etiological treatment available. Mesenchymal stromal cells (MSC) are used in clinical cell therapy for immunomodulation and tissue repair. In vitro priming is often used to improve the immunomodulation efficiency of MSC before administration.

Objective
Assess the effect of naive MSC (MSCn) or interleukin (IL)-1β primed (MSCp) treatment in a context of HS-induced organ injury.

Methods
Rats underwent fixed pressure HS and were treated with allogenic MSCn or MSCp. Liver and kidney injuries were evaluated 6h later by histological and biochemical analysis. Whole blood was collected to measure leukocytes phenotypes. Then, in vitro characterization of MSCn or MSCp was carried out.

Results
Plasma creatinine, blood urea nitrogen, and cystatin C were decrease by MSCp infusion as well as kidney injury molecule (KIM)-1 on histological kidney sections. Transaminases, GGT, and liver histology were normalized by MSCp. Systemic cytokines (IL-1α, IL-6, and IL-10) as well as CD80, 86, and PD-1/PDL-1 axis were decreased by MSCp on monocytes and granulocytes. In vitro, MSCp showed higher level of secreted immunomodulatory molecules than MSCn.

Conclusion
An early administration of MSCp moderates HS-induced kidney and liver injury. IL-1β priming improves MSC efficiency by promoting their immunomodulatory activity. These data provide proof of concept that MSCp could be a therapeutic tool to prevent the appearance of organs injury following HS.
Spatial point-pattern analysis as a powerful tool in identifying pattern-process relationships in plant ecology: an updated review
Authors: Ben-Said Mariem 
Published: August 2021

AbstractBackgroundEcological processes such as seedling establishment, biotic interactions, and mortality can leave footprints on species spatial structure that can be detectable through spatial point-pattern analysis (SPPA). Being widely used in plant ecology, SPPA is increasingly carried out to describe biotic interactions and interpret pattern-process relationships. However, some aspects are still subjected to a non-negligible debate such as required sample size (in terms of the number of points and plot area), the link between the low number of points and frequently observed random (or independent) patterns, and relating patterns to processes. In this paper, an overview of SPPA is given based on rich and updated literature providing guidance for ecologists (especially beginners) on summary statistics, uni-/bi-/multivariate analysis, unmarked/marked analysis, types of marks, etc. Some ambiguities in SPPA are also discussed.ResultsSPPA has a long history in plant ecology and is based on a large set of summary statistics aiming to describe species spatial patterns. Several mechanisms known to be responsible for species spatial patterns are actually investigated in different biomes and for different species. Natural processes, plant environmental conditions, and human intervention are interrelated and are key drivers of plant spatial distribution. In spite of being not recommended, small sample sizes are more common in SPPA. In some areas, periodic forest inventories and permanent plots are scarce although they are key tools for spatial data availability and plant dynamic monitoring.ConclusionThe spatial position of plants is an interesting source of information that helps to make hypotheses about processes responsible for plant spatial structures. Despite the continuous progress of SPPA, some ambiguities require further clarifications.
Model fusion of deep neural networks for anomaly detection
Authors: AlDahoul Nouar , Abdul Karim Hezerul , Ba Wazir Abdulaziz Saleh 
Published: August 2021

AbstractNetwork Anomaly Detection is still an open challenging task that aims to detect anomalous network traffic for security purposes. Usually, the network traffic data are large-scale and imbalanced. Additionally, they have noisy labels. This paper addresses the previous challenges and utilizes million-scale and highly imbalanced ZYELL’s dataset. We propose to train deep neural networks with class weight optimization to learn complex patterns from rare anomalies observed from the traffic data. This paper proposes a novel model fusion that combines two deep neural networks including binary normal/attack classifier and multi-attacks classifier. The proposed solution can detect various network attacks such as Distributed Denial of Service (DDOS), IP probing, PORT probing, and Network Mapper (NMAP) probing. The experiments conducted on a ZYELL’s real-world dataset show promising performance. It was found that the proposed approach outperformed the baseline model in terms of average macro Fβ score and false alarm rate by 17% and 5.3%, respectively.
Patient-reported outcome measures and clinical outcomes following peri-implant vestibuloplasty with a free gingival graft versus xenogeneic collagen matrix: a comparative prospective clinical study
Authors: Fu Xiaojiao , Wang Ying , Chen Bo , Tian Jiehua , Lin Ye , Zhang Yu 
Published: August 2021

Abstract
Background
The objective of this study was to compare patient-reported outcome measures (PROMs) and clinical outcomes after augmentation with xenogeneic collagen matrix (XCM) or free gingival graft (FGG) during different postoperative phases.

Methods
Forty-two patients (21 per group) with keratinized mucosa width (KMW) of < 2 mm at buccal implant sites in the posterior mandible were enrolled. All underwent vestibuloplasty and were allocated to either FGG (control) or XCM (test) group. Intraoperative morbidity of pain, stress, nausea, tolerance to time, and acceptance of surgery were evaluated immediately after surgery. The severity and duration of subjective pain, swelling, and bleeding were compared within a 2-week postoperative period. The willingness to retreat and satisfaction were assessed at 6 months. All PROMs were obtained using questionnaires and visual analog scales. The buccal KMW and other peri-implant parameters were also evaluated.

Results
No significant between-group differences were observed in PROMs immediately after surgery, except acceptance of surgery (0, 0–30.0 vs. 30, 0–50.0, p = 0.025). At 2 weeks, pain severity (46.7 ± 25.9 vs 61.9 ± 20.2, p = 0.040) and duration (5.52 ± 3.57 vs 8.48 ± 2.80, p = 0.005) were significantly lower in the test group, and pain perception during speaking and chewing was significantly higher for FGG, with no significant between-group differences in swelling and bleeding. At 6 months, the test group showed a higher willingness to retreat (76% vs 43%, p = 0.021); however, satisfaction with treatment outcomes was similar in both groups. At 6 months, the gain of KMW was significantly higher in FGG than in XCM (XCM: 1.57 ± 1.69 mm, FGG: 2.68 ± 1.80 mm, p = 0.003). Other peri-implant parameters did not show significant differences.

Conclusions
Within the limitation of the present nonrandomized study, XCM demonstrated more positive PROMs than FGG during different postoperative phases, mainly for less pain perception during the early healing stage, but was inferior to FGG in terms of gain of KMW. For KMW augmentation in the posterior mandible, XCM may be indicated when patients can bear little pain.

Clinical trial registration
ChiCTR1900022575, date of registration: 17/4/2019, retrospectively registered,
Breakfast characteristics, perception, and reasons of skipping among 8th and 9th-grade students at governmental schools, Jenin governance, West Bank
Authors: Badrasawi Manal , Anabtawi Ola , Al-Zain Yaqout 
Published: August 2021

Abstract
Background
There is growing recognition of the important role of breakfast in children’s nutrition, and the potential harms related to skipping breakfast, including its contribution to obesity and non-communicable diseases. The patterns associated with skipping breakfast may be related to the nutrition transition. This study aimed at exploring the composition of breakfast consumed by Palestinian school children and their perceptions toward this meal. It also aimed at exploring skipping breakfast prevalence, reasons, and its association with selected schoolchildren’s sociodemographic variables and behavioral patterns.

Methods
A cross-sectional online survey was conducted among 12- to 14-year-old schoolchildren from 4 governmental schools in urban and rural areas of the Jenin district in Palestine. The questionnaire included information about children and parents sociodemographic and behaviours, breakfast patterns and composition, reasons for skipping breakfast, and items on schoolchildren’s perception toward breakfast. Statistical analysis of the relevant factors was undertaken using SPSS software.

Results
In a sample of 193 schoolchildren, only 32% reported consuming breakfast all year round. The main reasons reported for skipping breakfast were not feeling hungry, not having the time, and lack of appetite. The vast majority (79%) believed breakfast was beneficial for general health. Sleeping before 10 pm, regular exercise (p value < 0.05). and shorter screen time were all significantly associated with a higher level of breakfast consumption (p value < 0.01).

Conclusion
Understanding the reasons for missing or skipping breakfast factors which make skipping it more likely, should inform public health strategies to promote breakfast consumption. For example, our findings suggest that awareness of the importance of breakfast was not a significant contributor to skipping breakfast, compared to other structural and cultural factors.
Multi-omics approach to precision medicine for immune-mediated diseases
Authors: Ota Mineto , Fujio Keishi 
Published: August 2021

AbstractRecent innovation in high-throughput sequencing technologies has drastically empowered the scientific research. Consequently, now, it is possible to capture comprehensive profiles of samples at multiple levels including genome, epigenome, and transcriptome at a time. Applying these kinds of rich information to clinical settings is of great social significance. For some traits such as cardiovascular diseases, attempts to apply omics datasets in clinical practice for the prediction of the disease risk have already shown promising results, although still under way for immune-mediated diseases. Multiple studies have tried to predict treatment response in immune-mediated diseases using genomic, transcriptomic, or clinical information, showing various possible indicators. For better prediction of treatment response or disease outcome in immune-mediated diseases, combining multi-layer information together may increase the power. In addition, in order to efficiently pick up meaningful information from the massive data, high-quality annotation of genomic functions is also crucial. In this review, we discuss the achievement so far and the future direction of multi-omics approach to immune-mediated diseases.
Machine learning for intrusion detection in industrial control systems: challenges and lessons from experimental evaluation
Authors: M. R. Gauthama Raman , Ahmed Chuadhry Mujeeb , Mathur Aditya 
Published: August 2021

AbstractGradual increase in the number of successful attacks against Industrial Control Systems (ICS) has led to an urgent need to create defense mechanisms for accurate and timely detection of the resulting process anomalies. Towards this end, a class of anomaly detectors, created using data-centric approaches, are gaining attention. Using machine learning algorithms such approaches can automatically learn the process dynamics and control strategies deployed in an ICS. The use of these approaches leads to relatively easier and faster creation of anomaly detectors compared to the use of design-centric approaches that are based on plant physics and design. Despite the advantages, there exist significant challenges and implementation issues in the creation and deployment of detectors generated using machine learning for city-scale plants. In this work, we enumerate and discuss such challenges. Also presented is a series of lessons learned in our attempt to meet these challenges in an operational plant.
Etiology of diarrheal disease among children under 5 years in Egypt: a high incidence of human bocavirus
Authors: Rizk Neveen M. , Abd-Elmaksoud Sherif , Farid Tarek M. , Abohashish Maha M. A. , Al-Herrawy Ahmad Z. , Hamza Ibrahim A. 
Published: August 2021

Abstract
Background
Human bocavirus (HBoV) is globally distributed and associated with respiratory and enteric infections. Limited data are available about the incidence of HBoV in Egyptian children. We aimed to investigate the association of HBoV genotypes in children with diarrheal disease and also to determine the possibility of HBoV co-infections with other human enteric pathogens.

Methods
A total of 102 stool samples were collected from children under five years old with diarrhea. Samples were analyzed for the presence of HBoV by real-time PCR. HBoV positive samples were tested for adenovirus (AdV), rotavirus (RoV), parasitic helminths, and enteric protozoa.

Results
HBoV was detected in 58% of examined cases. HBoV-3 was the most prevalent genotype observed (44%; 45 of 102), followed by HBoV-2/4 (33%; 34 of 102) and HBoV-1 (30%; 31 of 102). Although the incidence of HBoV was higher in males (66.6%; 34 of 51) than females (49%; 25 of 51), the analysis showed no significant difference for HBoV between genders. The average HBoV concentrations were 5.3 × 104 GC/g in males and 1.03 × 105 GC/g in females. Among the HBoV-positive samples, the single infection of HBoV was 52.5% (31/59), while the co-infections with multiple viruses were found in 1.7% (1/59) for HBoV and AdV, 33.9% (20/59) for HBoV and RoV, and 11.9% (7/59) for HBoV, and RoV and AdV. No co-infection with parasitic helminths or enteric protozoa was found.

Conclusions
The single infection of HBoV in some children suffering from acute gastroenteritis indicated that HBoV could be the main etiologic agent of the disease. The study highlights the high incidence of HBoVs genotypes with remarkable multiple co-infections in the pre-school children in Egypt.