bluuebunny/crossref_metadata_2025_split huggingface dataset:
Small molecule treatment alleviates photoreceptor cilia defects in LCA5-deficient human retinal organoids
Authors: Athanasiou Dimitra , Afanasyeva Tess A.V. , Chai Niuzheng , Ziaka Kalliopi , Jovanovic Katarina , Guarascio Rosellina , Boldt Karsten , Corral-Serrano Julio C. , Kanuga Naheed , Roepman Ronald , Collin Rob W.J. , Cheetham Michael E. 
Published: February 2025

Abstract

Bialleleic pathogenic variants in LCA5 cause one of the most severe forms of Leber congenital amaurosis, an early-onset retinal disease that results in severe visual impairment. Here, we report the use of gene editing to generate isogenic LCA5 knock-out (LCA5 KO) induced pluripotent stem cells (iPSC) and their differentiation to retinal organoids. The molecular and cellular phenotype of the LCA5 KO retinal organoids was studied in detail and compared to isogenic controls as well as patient-derived retinal organoids. The absence of LCA5 was confirmed in retinal organoids by immunohistochemistry and western blotting. There were no major changes in retinal organoid differentiation or ciliation, however, the localisation of CEP290 and IFT88 was significantly altered in LCA5 KO and patient photoreceptor cilia with extension along the axoneme. The LCA5-deficient organoids also had shorter outer segments and rhodopsin was mislocalised to the outer nuclear layer. We also identified transcriptomic and proteomic changes associated with the loss of LCA5. Importantly, treatment with the small molecules eupatilin, fasudil or a combination of both drugs reduced CEP290 and IFT88 accumulation along the cilia. The treatments also improved rhodopsin traffic to the outer segment and reduced mislocalisation of rhodopsin in the outer nuclear layer. The improvements in cilia-associated protein localisation and traffic were accompanied by significant changes in the transcriptome towards control gene expression levels in many of the differentially expressed genes. In summary, iPSC-derived retinal organoids are a powerful model for investigating the molecular and cellular changes associated with loss of LCA5 function and highlight the therapeutic potential of small molecules to treat retinal ciliopathies.
Differential Item Functioning in Preterm Infant Oral Feeding Readiness Assessment Scale in Neonates with critical conditions in NICU based on A Machine Learning Approach
Authors: Peng Kanglong , Gao Li , Cao Jianguo , Yun Guojun , Huang Yi 
Published: February 2025

Abstract

Aim
This study utilized the Rasch Model to test the psychometric properties of the Preterm Infant Oral Feeding Readiness Assessment Scale (PIOFRA) in neonates with critical conditions in neonate intensive care unit (NICU).
Methods
A total of 1419 infants were involved. Rasch Model (RM) was used to test the reliability and validity of the PIOFRA. The GPCMlasso Model was used to examine the differential item functioning (DIF).
Results
The involved samples responded to PIOFRA according to Rasch Model pattern. Disordered category function was found in PIOFRA. Besides, we identified one extra component in PIOFRA concerning the awake status. The DIF analysis revealed that gestational age and born weight only produced limit impact on neonates’ performance.
Conclusions
our result supports that FIOFRA is applicable for depicting the neurobehavior in high-risk infants with critical conditions in NICU. One drawback is found that the rating scale may jeopardize the measurement precision, further rating calibration is needed. Items in PIOFRA are displaying different DIF in corresponding subsamples (e.g. gestational age, born weight, oral feeding status). Future studies should focus on validating these findings in a more balanced sample which this study fails to achieve.
Can Climate Change Adaptation and Energy Efficiency Drive Economic Growth in Indonesia?
Authors: Sitepu Erica Ferry Sukma , Bina Maria Magdalena Lily , Soekarini Nyoman 
Published: February 2025

Abstract

Climate change and energy efficiency contribute to economic growth. This study aims to examine the impact of climate change and energy efficiency on economic growth across various regions in Indonesia, considering the influence of primary industrial sectors and green investment. The data used is panel data from 13 provinces during the 2014–2023 period. The method used is Mixed-Effect Maximum Likelihood Regression. The research results show that climate change has a significant negative impact on economic growth, especially in regions with a low primary industrial sector. Conversely, energy efficiency demonstrates a significant positive impact, particularly in regions characterized by substantial green investment and a dominant non-primary sector. However, energy efficiency does not exhibit a significant impact in regions with a highly developed primary industrial sector, highlighting the need for modernization within this sector. Meanwhile, inflation was found to have no significant impact on economic growth across all regional categories. Climate change mitigation strategies, including reducing carbon emissions, enhancing energy efficiency, and increasing investment in green infrastructure, are essential for fostering inclusive and sustainable economic growth.
Jell Classification: R11, P28, F43,C32
Enhancing English Proficiency Through an Intelligent Personal Assistant
Authors: Garg Meenakshi , Gadakh Anjali , Mudaliar Jayalaxmi , Sharma Anupama 
Published: February 2025

Abstract

This relative review investigates the coordination of artificial intelligence controlled advances in improving English language abilities, explicitly focusing on elocution and scholastic capability. It centers around the utilization of computer based intelligence driven devices like the Google Read So anyone might hear application, in mix with the Concentrated on Without holding back technique, to further develop jargon maintenance and elocution precision. The examination likewise explores the utilization of voice partners in refining scholastic English, featuring their true capacity for supporting language learning. The review exhibits the adequacy of Stowed away Markov Models (Gee) in man-made intelligence based discourse acknowledgment frameworks, which offer better outcomes in jargon maintenance thought about than conventional phonetic methodologies. This proposes that computer based intelligence driven advances have huge commitment for long haul language procurement. Besides, the use of Profound Brain Organizations (DNNs) is exhibited to upgrade both scholarly jargon and syntactic accuracy, helping students in conventional scholastic settings. Generally, the review highlights the worth of computer based intelligence in propelling elocution and scholastic language abilities, giving important experiences to language teachers and students the same.
Expression Profiling and Functional Characterization of LoNCED Gene in Pollen Abortion of Lilium spp.
Authors: Zhou Haiyan , Li Xiang , Ma Lan , Duan Qing , Du Wenwen , Cui Guangfen , Wu Xuewei , Jia Wenjie , Zhang Hao 
Published: February 2025

Abstract

Lilium spp. are widely cultivated for their ornamental value, but excessive pollen production poses commercial challenges. Male sterility, especially pollen-free cultivars, offers a promising breeding strategy to improve market appeal. ABA, a key hormone in regulating anther development, is influenced by the NCED gene. To investigate its role in male sterility, we isolated the LoNCED gene from both fertile and sterile lily progenies. After cloning and analyzing its tissue-specific expression, we explored its function through homologous transient overexpression and heterologous expression. Results showed that sterile progenies had significantly higher ABA levels, with LoNCED expression elevated in their anthers compared to fertile progenies. The LoNCED gene, with a 1812 bp open reading frame encoding 603 amino acids, encodes a hydrophilic protein (66.69 kDa) localized in chloroplasts and mitochondria. Sequence analysis revealed high similarity to NCED proteins from other species. Overexpression of LoNCED downregulated key anther development genes (DYT1, TDF1, CYP703A2, CALS5, DEX1) and caused tapetum degradation, abnormal microspore development, and pollen sterility. Transgenic Arabidopsis plants overexpressing LoNCED exhibited incomplete anther development, premature tapetum degradation, and reduced pollen grain production. These findings highlight the critical role of LoNCED in regulating anther sterility in lilies.
Scenario-based Evaluation of Landfill Methane Emission and Energy Generation Considering Diverse Waste and Seasonal Dynamics
Authors: Mayilsankar Balakrishnan , Megha Balakrishnan , Ramesh Srikrishnaperumal Thangam 
Published: February 2025

Abstract

Methane is a major constituent of landfill gas, which is emitted due to the anaerobic decomposition of municipal solid waste, contributing to the climate change impressions and global warming effects. However, it also holds the potential for energy generation, providing an opportunity to utilize this gas as a valuable energy resource. The current study aims to determine the potential methane emissions from the Ariyamangalam dumpsite in Tiruchirappalli, Tamilnadu taking into account the fresh waste disposal started in 2019. Waste disposal data spanning from 2019 to 2023 were collected, and future disposal will be forecasted, projecting a closure year of 2038. Estimating methane emissions was conducted through LandGEM version 3.03, SWEET version 4.0.3, and Afvalzorg multiphase gas emission tool considering existing waste disposal conditions and various framed waste diversion scenarios. The outcomes for existing waste disposal conditions reveal a peak methane emission in 2039, with values of 3242.32 Mg/year, 3027.29 Mg/year, and 4488.22 Mg/year for SWEET, Afvalzorg multiphase gas emission tool, and LandGEM respectively. Furthermore, the study reveals that diverting organic fractions and implementing effective recycling activities can significantly reduce methane emissions from the dumpsite. seasonal variations were observed through LandGEM, with lower emissions throughout the winter season and higher emissions in the monsoon season. Additionally, the total energy generation potential over 30 years was assessed, showing 187.89 GWh, 187.48 GWh, and 294.66 GWh based on the methane emissions data from the SWEET, Afvalzorg multiphase gas emission tool, and LandGEM tool respectively.
Tindak Tutur Direktif Pada Surat An-Nisa dan An-Nur dalam Al-Qur’an Terhadap Perempuan: Kajian Pragmatik
Authors: Nazifah Mutia Naura , Lukman Fahmy , Nugraha Tubagus Chaeru 
Published: February 2025

ملخص: يهدف المقال المعنون أفعال الكلام التوجيهي في سورتي النساء والنور في القرآن نحو النساء إلى تصنيف ووصف أنواع وأشكال الأفعال الكلامية الموجودة في سورتي النساء والنور في القرآن الكريم. -القرآن. يستخدم هذا البحث الأساليب التحليلية الوصفية ذات النهج النوعي، وطرق وتقنيات جمع البيانات في شكل أساليب الاستماع مع تقنيات تدوين الملاحظات، وطرق وتقنيات تحليل البيانات في شكل أساليب المطابقة مع تقنيات الفرز الواقعي، وكذلك الأساليب غير الرسمية و تقنيات عرض نتائج تحليل البيانات باستخدام الأوصاف. وأظهرت نتائج البحث أن هناك 16 نوعًا من أفعال الكلام الأمر، و6 أنواع من أفعال الكلام النهي، و7 أنواع من أفعال الكلام النصحية، ونوعين من أفعال الكلام الطلبية، ونوعين من أفعال الكلام التهديدية، ونوع واحد من أفعال الكلام الدعاءية. 31 شكلاً من أشكال أفعال الكلام التوجيهي المباشر و3 أشكال من أفعال الكلام غير المباشرة
الكلمات المفتاحية: القرآن؛ البراغماتية. أعمال الكلام التوجيهي؛ امرأة

Abstracts: The article entitled directive speech acts in suras An-Nisa and An-Nur in the Al-Qur'an towards women aims to classify and describe the types and forms of directive speech acts found in Surahs An-Nisa and An-Nur in the Al-Qur'an. an. This research uses descriptive analytical methods with a qualitative approach, data collection methods and techniques in the form of listening methods with note-taking techniques, data analysis methods and techniques in the form of matching methods with pragmatic sorting techniques, as well as informal methods and techniques for presenting the results of data analysis using descriptions. The research results showed that there were 16 types of commanding speech acts, 6 types of prohibiting speech acts, 7 types of advising speech acts, 2 types of requesting speech acts, 2 types of threatening speech acts, and 1 type of praying speech act. 31 forms of direct directive speech acts and 3 forms of indirect speech acts.
Keywords: Al-Qur'an; Pragmatics; Directive Speech Acts; Woman

Abstrak: Artikel yang berjudul tindak tutur direktif pada surat an-nisa dan an-nur dalam al-qur’an terhadap perempuan bertujuan untuk mengklasifikasi dan mendeskripsikan jenis dan bentuk tindak tutur direktif yang terdapat pada surah An-Nisa dan An-Nur dalam Al-Qur’an. Penelitian ini menggunakan metode deskriptif analitis dengan pendekatan kualitifatif, metode dan teknik pengumpulan data berupa metode simak dengan teknik catat, metode dan teknik analisis data berupa metode padan dengan teknik pilah pragmatis, serta metode dan teknik penyajian hasil analisis data berupa informal menggunakan deskripsi. Hasil penelitian terdapat 16 jenis tindak tutur memerintah, 6 jenis tindak tutur melarang, 7 jenis tindak tutur menasihati, 2 jenis tindak tutur meminta, 2 jenis tindak tutur mengancam, 1 jenis tindak tutur ber’doa. 31 bentuk tindak tutur direktif langsung dan 3 bentuk tindak tutur tidak langsung.
Kata Kunci: Al-Qur’an; Pragmatik; Tindak Tutur Direktif; Perempuan
Supporting decision-making for industrial symbioses using a hybrid modelling approach and its application to wastewater treatment
Authors: Chen Otto , Mustafee Navonil , Evans Barry , Khoury Mehdi , Vamvakeridou-Lyroudia Lydia , Chen Albert S. , Djordjević Slobodan , Savić Dragan 
Published: February 2025

ABSTRACT

Industrial Symbiosis (InSym) capitalises on the proximity of entities to gain a competitive advantage through collective strategies. Within the Circular Economy, this involves the circular exchange and reuse of water, energy, and resources among participating businesses, enhancing resource valorisation in manufacturing. However, as a distinct business model, InSym requires collaboration among multiple stakeholders working toward a shared goal, posing challenges in achieving mutually beneficial outcomes. Operations Research (OR) – particularly computer modelling and simulation techniques – can help mitigate risks in InSym implementation by enabling an experimental approach to decision-making. This paper presents a hybrid modelling framework to support InSym decision-making. The framework integrates four OR techniques: Agent-Based Simulation (ABS), Discrete-Event Simulation (DES), System Dynamics (SD), and Multiple Criteria Decision Analysis (MCDA) to develop a hybrid InSym model. ABS captures stakeholder behaviour, DES simulates operational processes, SD represents dynamic interactions, and MCDA incorporates stakeholder perspectives. The model evaluates collective treatment strategies for olive mill wastewater, addressing key challenges such as scattered small-scale olive mills, seasonal wastewater discharge, and high organic loading. This innovative framework addresses InSym decision-making at operational, tactical, and strategic levels, transforming the economy-environment dilemma into a win-win scenario for olive oil businesses and local authorities.
Natural Hazard Risk Assessments Applicable to Enterprise Risk Management
Authors: Keaton Jeffrey R. , Munro Rosalind 
Published: February 2025

ABSTRACT
Current methods for assessing risks from earthquake and many weather-related hazards are appropriate to inform decision makers and stakeholders of likely future impacts. However, methods for assessing landslide and volcanic risks are insufficient. The U.S. Federal Emergency Management Agency’s National Risk Index (NRI) considers 10 weather, four weather-triggered, one earthquake-triggered, and three geologic hazards. Earthquake and some weather-related hazards include regional design requirements for building performance, and accurate forecasting is available for evacuation in advance of extreme weather. Teams of engineers and scientists examine building and infrastructure performance following major earthquakes, hurricanes, tornadoes, and floods. Performance review lessons become integrated into subsequent building-code revisions. Landslide damage depends on local topography, geology, and land use. Building codes, based on stable-site concepts, have no design provisions for ground deformation, and post-landslide observations focus on ground deformation without considering building performance or resilience. Volcanic activity effects range from tranquil lava flows to violent ash eruptions to rapid debris avalanches and lahars. The NRI considers volcanic effects only close to sources; consequently, damaging effects of distant volcanic ash eruptions are not considered. Enterprise risk management (ERM) seeks to identify, analyze, counter, and control the spectrum of internal and external risks to business enterprises, including distant volcanic ash sources in North America and landslides disrupting key supply chain routes in Asia. Considering possible natural hazard effects from an ERM site-location perspective could be as beneficial as semi-quantitative risk analyses for high-hazard facilities. Properly trained geologists are well-suited to participate in or lead natural hazard assessments.
Evidence for a Single Holocene Paleoseismic Event on the Pajarito Fault, Northern New Mexico
Authors: Crawford Brandon M. , Swanson Erika M. , Schultz-Fellenz Emily S. , Givler Robert , Baldwin John , Lettis William , Kelley Shari , Koning Daniel , Olig Susan , Rockwell Thomas , Goehring Brent M. , Hartleb Ross , Bloszies Christopher , Gray Brian 
Published: February 2025

ABSTRACT
Low-slip rate fault systems tend to be less studied than their high-slip rate counterparts, and paleoseismic techniques used to study them may pose challenges in interpretation that differ from high-slip rate systems. A good example of this is the Pajarito fault system (PFS), a normal fault complex within the Rio Grande rift. Despite numerous previous paleoseismic trenching studies conducted on the PFS between 1990 and 2003, considerable uncertainty remains regarding its Holocene paleoseismic history, particularly for the primary Pajarito fault (PF). To further clarify the PF paleoseismic history, we present data from paleoseismic investigations of 6 trenches at 3 distinct locations along the PF. Though the totality of the age and structural data obtained in this study is complex and not entirely consistent with any one interpretation, a single Holocene paleoearthquake occurring younger than ∼1,600 to 2,300 kcal yr BP is the simplest interpretation. It is possible that the PF records two Holocene events, with a penultimate event 6.9–2.4 kcal yr BP event and the aforementioned most recent event (MRE) between 2.3 and 1.6 kcal yr BP. However, only a single wall of one trench, out of a total of 12 walls in our 6 trenches, provides evidence supporting that interpretation. This study finds evidence of a single late Holocene paleoseismic event on the PF and sparse evidence for 2 Holocene paleoseismic events on the PF and highlights the benefits of logging multiple trench walls to better understand the complexity that results from this low-slip rate, low-deposition-rate fault system.
Enhancing Onion Yield Through Integrated Weed Management Tactics in the Middle Awash Valley, Ethiopia
Authors: Getahun Sileshi , Alemu Zemedkun , Seid Nurhussein 
Published: February 2025

Las malas hierbas juegan un rol importante en la reducción de la producción y la productividad de la cebolla en Etiopía. Existe poca información sobre la gestión en las tierras bajas y cálidas de Ethiopia. Este estudio evalúa la eficacia de las tácticas integradas de control de las malas hierbas en los cultivos de cebolla. En el campo, los tratamientos se dispusieron en un diseño de bloques completos aleatorizados (DBCA) con tres repeticiones. Los datos recogidos fueron las especies de malas hierbas, la densidad y el peso seco de las malas hierbas, además, el rendimiento de la cebolla y los rasgos de rendimiento en la madurez. El campo experimental estaba infestado por especies de malas hierbas herbáceas y de hoja ancha incluida la juncia, la especie de mala hierba predominante. El resultado del rendimiento de la cebolla y rasgos de rendimiento fueron significativamente (p < 0.05) diferentes entre tratamientos. La azada a tres manos y la Pendimetalina 500 SC + Oxifluorfeno 24 EC apoyado por escarda a una mano en el día 70 después de de trasplante demostraron una eficacia superior en el control de las malas hierbas, lo que se tradujo en un aumento del rendimiento de los bulbos de cebolla. rendimiento de los bulbos de cebolla. El análisis coste-beneficio indicó que estos dos métodos de control de las malas hierbas ofrecían la mejor relación coste-beneficio (15.84 y 13.25 respectivamente) y se recomiendan para la gestión de malas hierbas en la producción de cebolla. El uso de pendimetalina en preemergencia seguida de la aplicación en el momento oportuno de Oxyfluorfen 24 EC y la escarda manual después del trasplante puede suprimir eficazmente las malas hierbas y mejorar la eficacia de su control.
Investigation of Pre-Service Science Teachers’ Social Media Usage Preferences:
Authors: Said Doğru M. , Yüzbaşioğlu Fatih 
Published: February 2025

Abstract
Today, social media is widely used for many purposes, from socialization to communication and education to commerce. This study examines the social media use of pre-service science teachers in the context of creativity and the Big Five Personality Traits Theory. For this study, the participants were pre-service science teachers selected to represent the target group for examining the research objectives. Data from science teachers included in the study group were collected through an online questionnaire using purposive sampling, allowing for a focused examination of specific characteristics within this group. The data obtained from the questionnaires were analyzed and interpreted using an integrated SEM-ANN method. The findings obtained as a result of the study revealed that creativity and personality traits in the Big Five Personality Traits Theory affect the social media use of pre-service science teachers.