bluuebunny/crossref_metadata_2025_split huggingface dataset:
Does Inter-Border Conflict Influence the Views of Task Sharing Among Community Health Volunteers in Nigeria? a Qualitative Study
Authors: Lar Luret Albert , Stewart Martyn , Isiyaku Sunday , Dean Laura , Ozano Kim , Mpyet Caleb , Theobald Sally 
Published: December 2021

Abstract
Background: Volunteer community health workers are increasingly being engaged in Nigeria, through the World Health Organization’s task sharing strategy. This strategy aims to address gaps in human resources for health, including inequitable distribution of health workers. Recent conflicts in rural and fragile border communities in northcentral Nigeria create challenges for volunteer community health workers to meet their communities increasing health needs. This study aimed to explore the perception of volunteers involved in task sharing to understand factors affecting performance and delivery in such contexts.Methods: Eighteen audio recorded, semi-structured interviews with volunteers and supervisors were conducted. Their perceptions on on how task sharing and allocation affect performance and delivery were elucidated. The transactional social framework was applied during the thematic analysis process to generate an explanatory account of the research data.Results: Promotive and preventive tasks were shared among the predominantly agrarian respondents. There was a structured task allocation process that linked the community with the health system and mainly cordial relationships were in place. However, there were barriers related to ethnoreligious crises and current conflict, timing of task allocations, gender inequities in volunteerism, shortage of commodities, inadequate incentives, dwindling community support and negative attitudes of some volunteers.Conclusion: The perception of task sharing was mainly positive, despite the challenges, especially the current conflict. In this fragile context, reconsideration of non-seasonal task allocations within improved community-driven selection and security systems should be encouraged. Supportive supervision and providing adequate and timely renumerations will also be beneficial in this fragile setting.
Upregulation of P2Y12 Inhibits Chondrocyte Apoptosis in Lumbar Osteoarthritis Through PI3K/AKT Signaling Pathway
Authors: Wu Guanyin , Xue Pengfei , Zhang Mo , Gui Chao , Bao Guofeng , Xu Guanhua , Jiang Jiawei , Zhang Jinlong , Cui Zhiming , Jinhu rich , Cui Shengyu , Sun Yuyu 
Published: December 2021

Abstract
Lumbar facet osteoarthritis (FJOA) is one of the major causes of severe low back pain and disability worldwide. However, the underlying mechanism of cartilage degeneration in FJOA remains unclear. The purpose of this study was to investigate the expression of P2Y12 in FJOA and its possible role. Changes of chondrocytes in rat facet joints with degenerative changes were observed by HE and ferro red solid green staining. The expression changes of P2Y12, MMP13 and COL2 in FJOA were observed by immunohistochemistry. In vitro, human SW1353 chondrosarcoma cells were stimulated with IL-β to establish chondrocyte apoptosis model. Western blot analysis showed that P2Y12 and cleaved caspase-3 were significantly expressed in SW1353 cells. Co-localization of P2Y12-cleaved Caspase-3 in apoptosis model was detected by double-standard immunofluorescence staining. We demonstrated that P2Y12 may have an anti-apoptotic effect in FJOA chondrocytes apoptosis by inhibiting the expression of P2Y12 by siRNA. In addition, flow cytometry showed that P2Y12 gene knockout enhanced apoptosis induced by P2Y12. Our data suggest that P2Y12 has a chondroprotective effect on FJOA.
Temporal Monitoring and Predicting of the Abundance of Malaria Vectors Using Time Series Analysis of Remote Sensing Data through Google Earth Engine
Authors: Youssefi Fahimeh , Zoej Mohmmad Javad Valadan , Hanafi-Bojd Ahmad Ali , Darian Alireza Borhani , Khaki Mehdi , Nezhad Alireza Safdari 
Published: December 2021

Abstract

Background: In many studies in the field of malaria, environmental factors have been acquired in single-time, multi-time or a short time series using remote sensing and meteorological data. Selecting the best periods of the year to monitor the habitats of Anopheles larvae can be effective in better and faster control of malaria outbreak.Methods: In this article, high-risk times for three regions in Iran, including Qaleh-Ganj, Sarbaz and Bashagard counties with history of malaria prevalence had been estimated. For this purpose, a series of environmental factors affecting the growth and survival of Anopheles had been used over a seven-year period through the GEE. Environmental factors used in this study include NDVI and LST extracted from Landsat-8 satellite images, daily precipitation data from PERSIANN-CDR, soil moisture data from NASA-USDA Enhanced SMAP, ET data from MODIS sensor, and vegetation health indices included TCI and VCI extracted from MODIS sensors. All these parameters were extracted on a monthly average for seven years and, their results were fused at the decision level using majority voting method to estimate high-risk time in a year.Results: The results of this study indicated that there were two high-risk times for all three study areas in a year to increase the abundance of Anopheles mosquitoes. The first peak occurred from late winter to late spring and the second peak from late summer to mid-autumn. If there is a malaria patient in the area, after the end of the Anopheles larvae growth period, the disease will spread throughout the region. Further evaluation of the results against the entomological data available in previous studies showed that the high-risk times predicted in this study were consistent with the increase in the abundance of Anopheles mosquitoes in the study areas. Conclusions: The proposed method is very useful for temporal prediction of the increase of the abundance of Anopheles mosquitoes and also the use of optimal data with the aim of monitoring the exact location of Anopheles habitats. This study extracted high-risk time based on the analysis of the time series of remote sensing data.
High Expression of EIF4G2 Mediated By the TUG1/hsa-miR-26a-5p Axis Is Associated With Poor Prognosis and Immune Infiltration of Gastric Cancer
Authors: Fu Liu , Wang Zhe , Jiang Fengxiang , Wei Guohua , Sun Longe , Guo Chuanyong , Wu Jianye , Zhu Jianhuan 
Published: December 2021

Abstract
Background: Eukaryotic translation initiation factor 4 gamma 2 (EIF4G2) is involved in the occurrence and development of various tumors. However, the effect of EIF4G2 in gastric cancer (GC) has not been fully explored. The purpose of this study was to explore the function and mechanism of EIF4G2 in GC.Methods: The Tumor Immune Estimation Resource 2.0 database was used to analyze EIF4G2 expression in various cancers and the relationship between EIF4G2 expression and tumor-infiltrating immune cells. Gene Expression Profiling Interactive Analysis was utilized to assess the EIF4G2 expression level and its effect on survival in GC. UALCAN was conducted to analyze EIF4G2 expression in various sub-groups of GC. The Kaplan–Meier plotter was employed for survival analysis. Receiver Operator Characteristic (ROC) curve analysis was applied to evaluate the diagnostic role of EIF4G2 in GC. LinkedOmics was used to identify the co-expressed genes and Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathways. The Tumor-Immune System Interaction database was employed to analyze the correlation between EIF4G2 expression and tumor-infiltrating lymphocytes. The starBase web platform was used to predict the upstream microRNAs and long non-coding RNAs.Results: EIF4G2 expression was upregulated in GC tissues compared to normal controls. High expression of EIF4G2 indicated poor prognosis in GC. ROC analysis revealed that EIF4G2 had good diagnostic ability to distinguish GC from normal tissues. Immune infiltration analysis indicated that EIF4G2 expression may be involved in the modulation of tumor immune infiltration in GC. Finally, we determined that the Taurine Up-Regulated 1 (TUG1)/hsa-miR-26a-5p/EIF4G2 axis was the most likely regulatory pathway involved in GC development.Conclusions: EIF4G2 was upregulated in GC and elevated expression of EIF4G2 indicated unfavorable prognosis. Moreover, EIF4G2 expression may be involved in the regulation of tumor immune cell infiltration. The TUG1/hsa-miR-26a-5p axis is a likely upstream regulatory mechanism of EIF4G2 in GC. EIF4G2 may thus serve as a prognosis biomarker and present a new therapeutic target.
Visual LAMP Method for Detection of Vibrio vulnificus in Aquatic Products and Environmental Water
Authors: Tian Zhuo , Yang Lili , Qi Xin , Zheng Qiuyue , Shang Dejing , Cao Jijuan 
Published: December 2021

Abstract
Background A visualized, rapid, simple method was developed based on loop-mediated isothermal amplification (LAMP) assay to detect Vibrio vulnificus in aquatic products and aquaculture waters.Results Genomic DNA was extracted from Vibrio vulnificus using the boiling and column extraction methods and optimized primers were used to detect the gyrB gene using a visual LAMP method. The sensitivity of the assay was 10 fg/μL, and the results were stable and reliable. Of 655 aquatic product samples and 558 aquaculture waters samples, the positive rates of Vibrio vulnificus detection were 9.01% and 8.60%, respectively, which is markedly higher than that of the traditional culture identification methods. Conclusion The relatively simple technical requirements, low equipment costs, and rapid detection time make the visual LAMP method for detection of Vibrio vulnificus a convenient choice for field diagnosis in the aquaculture industry.
Semiconductor Two-Dimensional PdQ2 (Q=S, Se) Monolayer: Strain Modulating Electronic Band Gaps and SQ Efficiencies
Authors: Gajjar P , Raval Dhara , Gupta Sanjeev , Ahuja Rajeev 
Published: December 2021

Abstract
We studied the physical, electronic transport and optical properties of a unique pentagonal PdQ2 (Q= S, Se) monolayers. The dynamic stability of 2D - wrinkle like - PdQ2 is proven by positive phonon frequencies in the phonon dispersion curve. The optimized structural parameters of wrinkled pentagonal PdQ2 are in good agreement with the available experimental results. The ultimate tensile strength (UTHS) was calculated and found that, penta-PdS2 monolayer can withstand up to 16% (18%) strain along x (y) direction with 3.44 GPa (3.43 GPa). While, penta-PdSe2 monolayer can withstand up to 17% (19%) strain along x (y) dirrection with 3.46 GPa (3.40 GPa). It is found that, the penta-PdQ2 monolayers has the semiconducting behavior with indirect band gap of 0.94 and 1.26 eV for 2D-PdS2 and 2D-PdSe2, respectively. More interestingly, at room temperacture, the hole mobilty (electron mobility) obtained for 2D-PdS2 and PdSe2 are 67.43 (258.06) cm2 V-1 s-1 and 1518.81 (442.49) cm2 V-1 s-1, respectively. In addition, I-V characteristics of PdSe2 monolayer show strong negative differential conductance (NDC) region near the 3.57 V. The Shockly-Queisser (SQ) effeciency prameters of PdQ2 monolayers are also explored and the highest SQ efficeinciy obtained for PdS2 is 33.93% at -5% strain and for PdSe2 is 33.94% at -2% strain. The penta-PdQ2 exhibits high optical absorption intensity in the UV region, up to 4.04 × 105 (for PdS2) and 5.28 × 105 (for PdSe2), which is suitable for applications in optoelectronic devices. Thus, the ultrathin PdQ2 monolayers could be potential material for next-generation solar-cell applications and high performance nanodevices.
Biogenic Synthesis of Silver Nanoparticles Mediated by the Consortium Comprising the Marine Fungal Filtrates of Penicillium Oxalicum and Fusarium Hainanense Along With Their Antimicrobial, Antioxidant and Larvicidal Potency
Authors: Thakor Rashmi , Mistry Harsh , Patel Hitesh , Bariya Himanshu S 
Published: December 2021

Abstract
The silver nanoparticles were biosynthesized using fungal isolates as well as their mixed cell free filtrate acting as a consortium namely, DS-2 (Penicillium oxalicum), DW-8 (Fusarium hainanense) and DSW-28, respectively. The UV-Visible spectra determined the surface plasmon resonance at 438, 441 and 437 nm for the silver nanoparticles synthesized by DS-2, DW-8 and DSW-28, respectively. The Tauc’s plot analysis disclosed the band gap energy between 2.21 eV to 2.24 eV which depicted their ability to act as a semiconductor. The TEM imaging revealed the spherical shape along with the average particle size of DS-2 as 11.14 ± 2.39 nm and DW-8 as 7.59 ± 1.31 nm whereas that of DSW-28 as 5.73 ± 0.4 nm. Thus, the silver nanoparticles synthesized by DSW-28 were smaller in size than the individual isolates. The XRD pattern of the silver nanoparticles exhibited the crystalline structure corresponding to their peaks. The FTIR spectra indicates the presence of different functional groups on the surface of the synthesized silver nanoparticles. The broad-spectrum antimicrobial activity was exhibited by silver nanoparticles synthesized by DSW-28 against Gram positive, Gram negative bacteria and plant pathogen Fusarium oxysporum than the individual fungal isolates. The DSW-28 synthesized silver nanoparticles also acts as an effective antioxidant by depicting their radical scavenging activity against DPPH. Moreover, the silver nanoparticles synthesized by DSW-28 also inhibited the growth of 4th instar larvae of Aedes aegypti and Culex quinquefasciatus more efficiently than DS-2 and DW-8 in a dose-dependent method. The impressive bioactivity of the silver nanoparticles synthesized by the mixture of cell free filtrate of various fungi acting as a consortium recommends their prospective use in agriculture as well as in biomedical as an antimicrobial, antioxidant and larvicidal agents in future.
Nano-Fe (Magnetic-Fe) and Se Foliar Application Tranquilize the Salinity Adverse-Effects on Satureja Mutica Fisch and Satureia Spicigera (C. Koch) Boiss
Authors: Hassanpouraghdam Mohammad Bagher , Mehrabani Lamia Vojodi , Masoumpour Zahra , Soltanbeigi Amir , Egamberdieva Dilfuza 
Published: December 2021

Abstract
Background: The secondary metabolites from savory species are widely used in food and pharmaceutical industries. Salt accumulation in the growing medium adversely affects the growth and yield of plants. The hyper-availability of Na+ and Cl- triggers nutrient imbalances, leading to secondary ionic stress. Under salinity exposure, the reactive oxygen species (ROS) over-generation drives oxidative stress in cells. Moreover, when facing environmental stress factors; the availability of essential nutrients and especially micro-elements strongly declines. Foliar application of micro-nutrients principally as nano-form is a promising strategy in meeting the nutritional demands of plants under stress environments with progressive nutrient shortages. Nano-materials and the supply of nutrients as foliar treatments meliorate the growth, biochemical reactions, and nutrient use efficiency of plants under salinity. The idea with the present experiment was to assay the effects of nano-Fe (magnetized-Fe) and selenium foliar application on the growth and some physiological responses of two Satureja species under saline-sodic conditions.Results: When studying the foliar application of Se and nano-Fe (0 and 3 mg L-1) on Satureja mutica and Satureia spicigera via two separate experiments, under normal no-saline conditions; the highest catalase activity was recorded in magnetized-Fe treated plants in both species. Independent effects of foliar application and plant species influenced total phenolics and Mg content of leaves. Foliar sprays reduced MDA content in plant tissue. In the second experiment, foliar applications were evaluated under salinity conditions. No-saline × Se and magnetized-Fe treated plants attained the highest data for aerial parts biomass in S. spicigera.Conclusion: The results demonstrated that salinity adversely influenced the growth and physiological responses, nevertheless, foliar spray with Se and magnetized-Fe partially ameliorated the salinity depression on Satureja species.
The therapeutic effects of larval excretion/secretion of Lucilia sericata on Leishmania major under in vitro and in vivo conditions
Authors: Sherafati Jila , Dayer Mohammad Saaid , Ghaffarifar Fatemeh 
Published: December 2021

Abstract
Background

Leishmaniasis is a neglected infectious disease caused by a kinetoplastid protozoan. The disease generally manifests as characteristic skin lesions. Due to the lack of definitive treatment and drugs without side effects, many studies have focused on natural compounds as promising drugs for its treatment. This study aimed to evaluate the effects of larval excretion/secretion products (ES) of Lucilia sericata in crude and fractionated forms on Leishmania parasites under both in vitro and in vivo conditions.
Methods

In vitro experiments involved evaluation of ES products on both promastigotes and amastigotes inside infected macrophages, whereas in vivo experiments included comparative treatments of Leishmanial lesions of mice using Eucerin-formulated ES products and glucantime.
Results

The IC50 values were 38.7 µg/ml, 47.6 µg/ml, 63.3 µg/ml, and 29.1 µg/ml for crude ES, over 10 kDa ES-fraction, under 10 kDa ES-fraction, and glucantime respectively. Significant differences were observed between viability percentages of promastigotes treated with crude ES and its fractions compared to negative control (p < 0.0001). Crude ES was more effective on amastigote than other two ES fractions at 300 µg/ml concentration. Macroscopic measurement of lesion sizes revealed that the reduction of lesion size in mice treated with crude ES followed quicker cascades of healing than in those treated with glucantime and fractionated ES.
Conclusion

The present study showed that larval ES of Lucilia sericata in both crude and fractionated forms are effective on both intracellular and extracellular forms of L. major. It also provided evidence that the larval ES exerts both topical and systemic therapeutic effects on leishmanial lesions of the model animal.
Class I HDAC Inhibitor Rescues Synaptic Damage and Neuron Loss in APP-Transfected Cells and APP/Ps1 Mice through the GRIP1/AMPA Pathway
Authors: Han Ying , Chen Le , Liu Jingyun , Wang Chunyang , Guo Yu , Yu Xuebin , Zhang Chenghong , Chu Haiying , Ma Haiying 
Published: December 2021

Abstract
As a neurodegenerative disease, Alzheimer's disease (AD) seriously affects the health of older people. It is now known that changes in synapses occur first in the course of disease, perhaps even before the formation of Aβ plaques. Histone deacetylase (HDAC) can mediate the damage of Aβ oligomers to dendritic spines. Therefore, we examined the relationship between HDAC activity and synaptic defects by using an HDACI, BG45 in Human neuroblastoma SH-SY5Y cell line with stable overexpression of Swedish mutant APP (APPsw) and in APP/Ps1 transgenic mice during this study. The cells were treated with 15µM BG45 and the APP/Ps1 mice 30mg/kg BG45. We detected the level of synapse-related proteins, HDACs, tau phosphorylation and AMPA receptors by western bloting and immunohistochemistry. We also measured the expression of cytoskeletal proteins in the cell model. The mRNA level of GRIK2, SCN3B, SYNPR, Grm2, Grid2IP, GRIP1,GRIP2 were. to explore the effects of HDACi on regulating the synaptic proteins and AMPA receptors. Our studies demonstrated that the expression of HDAC1、HDAC2 and HDAC3 was increased, which was accompanied by the downregulation of the synapse-related proteins synaptophysin (SYP), postsynaptic dendritic protein (PSD-95) and spinophilin as early as 24 h after transfection with APPsw gene. BG45 upregulated the expression of synapse-related proteins and repaired cytoskeletal damage. In vivo, BG45 alleviated the apoptotic loss of hippocampal neurons, upregulated synapse-related proteins, reduced Aβ deposition and phosphorylation of tau and increased the level of the synapse-related genes GRIK2, SCN3B, SYNPR, Grm2, and Grid2IP. BG45 increased the expression of the AMPA receptor subunits GluA1, GluA2 and GluA3 on APPsw-transfected cells and increased GRIP1 and GRIP2 expression and AMPA receptor phosphorylation in vivo. These results suggest that HDACs are involved in the early process of synaptic defects of AD and that BG45 may rescue synaptic damage and loss of hippocampal neurons by specifically inhibiting HDAC1、HDAC2 and HDAC3, thereby modulating AMPA receptor transduction, increasing synapse-related gene expression and finally improving excitatory synapses. BG45 may be considered as a potential drug for the treatment of early AD for further study.
New behaviors of α-particle preformation factors near doubly magic 100Sn
Authors: Deng Jungang , Sun Xiaodong , Zhang Hongfei 
Published: December 2021

Abstract
The α-particle preformation factors of nuclei above doubly magic nuclei 100Sn and 208Pb are investigated.The results show that the α-particle preformation factors of nuclei near self-conjugatedoubly magic 100Sn are larger significantly than those of analogous nuclei just above 208Pb, andthey will be enhanced as the nuclei move towards the N = Z line. The correlation energy of theproton-neutron Ep-n and two protons-two neutrons E2p-2n of nuclei near 100Sn also exhibit similarsituations indicating that the interactions between protons and neutrons occupying similar single-particleorbitals could enhance the α-particle preformation factors and result in the superallowedα decay. It also provides evidence of the significant role of proton-neutron interaction on α-particlepreformation. Besides, the linear relationship between α-particle preformation factors and the productof valence protons and valence neutrons for nuclei around 208Pb is broken in the 100Sn regionbecause the α-particle preformation factor is enhanced when the nucleus near 100Sn moves towardsthe N = Z line. Furthermore, the calculated decay half-lives can well reproduce the experimentaldata including the recent observed self-conjugate nuclei 104Te and 108Xe [Phys. Rev. Lett. 121,182501 (2018)].
Optimising the Photothrombotic Model of Stroke in the C57BI/6 and FVB/N Strains of Mouse
Authors: Knezic Adriana , Broughton Brad , Widdop Robert , McCarthy Claudia 
Published: December 2021

Abstract
The photothrombotic stroke model relies on the interaction between photosensitive-dye and light for clot formation. Interestingly, the relationship between the length of light exposure and stroke-outcome has never been examined. This model has yet to be established in the FVB/N strain, even though stroke-outcomes are strain-specific. Therefore, this study aimed to examine the effect of different lengths of light exposure in two strains of mice on photothrombotic stroke. Male FVB/N and C57Bl/6 mice were subjected to stroke using 15, 18, or 20-mins light exposure. Mice underwent functional testing for up to 7 days. Infarct volume was assessed with thionin staining, and cellular responses to injury analysed via immunofluorescence. Blood brain barrier (BBB) breakdown was assessed using Evans blue dye. Increasing light exposure from 15 to 20-minutes increased infarct volume but not functional deficit. Interestingly, there were strain-specific differences in functional outcomes, with FVB/N mice having less deficit on the hanging wire test than C57BI/6 after 15-minutes of light exposure. The opposite was seen in the adhesive removal test. There was no difference in number of neurons, astrocytes, microglia, macrophages, and T cells between the strains, despite FVB/N mice demonstrating greater BBB breakdown and an enlarged spleen post-stroke. Increasing light exposure systematically increases infarct volume but does not worsen functional outcomes. FVB/N and C57Bl/6 mice exhibit subtle differences in functional outcomes post stroke, which highlights the need to choose tests which are appropriate for the mouse strain being used.