bluuebunny/crossref_metadata_2025_split huggingface dataset:
Scratching the discs: evaluating alternative hypotheses for the origin of the Ediacaran discoidal structures from the Cerro Negro Formation, La Providencia Group, Argentina
Authors: Inglez Lucas , Warren Lucas V. , Quaglio Fernanda , Netto Renata G. , Okubo Juliana , Arrouy Maria J. , Simões Marcello G. , Poiré Daniel G. 
Published: May 2021

AbstractIn the Ediacaran marine succession of the Cerro Negro Formation (Tandilia System, NE Argentina), abundant microbially induced sedimentary structures indicate general conditions of substrate biostabilization. Numerous discoidal structures in this succession were previously interpreted as moulds of soft-tissue holdfasts of sessile organisms, within the form genus Aspidella. In this study, we performed a detailed re-analysis of some of these features and discuss two alternative hypotheses to explain their genesis: (1) as the result of soft-sediment deformation and fluid injection structures; and (2) as structures of active animal–sediment interaction (i.e. trace fossils). We show that the dome-shaped discs are internally laminated, with a cylindrical to a funnel-shaped vertical tube at their central region. The presence of these downwards vertical extensions and other intricate internal arrangements cannot be explained under the taphonomic spectrum of discoidal fossils, but shows striking similarities to Intrites-like structures and other sand-volcano-like pseudofossils (e.g. Astropolithon). However, some structures are hard to distinguish from vertical dwelling burrows with funnel-shaped apertures and thick-lined walls, commonly produced by suspension- and detritus-feeding invertebrates (e.g. Skolithos isp., Monocraterion isp. and, less likely, Rosselia isp.). Since reliable age constraints are unavailable, and further investigation concerning other palaeobiological indicators is needed, the most parsimonious hypothesis is that of a structure derived from fluid-escape processes. Our study demonstrates the importance of detailed investigation on discoidal structures in either upper Ediacaran or lower Cambrian strata.
Myelin regulatory factor deficiency is associated with the retinal photoreceptor defects in mice
Authors: Yu Xiaowei , Sun Nannan , Yang Xue , Zhao Zhenni , Zhang Jiamin , Zhang Miao , Zhang Dandan , Ge Jian , Fan Zhigang 
Published: May 2021

Abstract
Previously, we reported the myelin regulatory factor (MYRF) as a candidate gene for nanophthalmos. We have also produced Myrf knockdown (Myrf+/−) mouse strain to investigate the cellular and molecular phenotypes of reduced MYRF expression in the retina. Myrf+/− mouse strain was generated using the CRISPR/Cas9 system. Optomotor response system, electroretinogram (ERG), spectral-domain optical coherence tomography (SD-OCT), histology, and immunohistochemistry were performed to evaluate retinal spatial vision, electrophysiological function, retinal thickness, and pathological changes in cone or rod photoreceptors, respectively. RNA sequencing (RNA-seq) was performed to investigate the underlying molecular mechanism linking Myrf deficiency with photoreceptor defects. The genotype and phenotype of CRISPR/Cas9-induced Myrf+/− mice and their offspring were comprehensively investigated. Photoreceptor defects were detected in the retinas of Myrf+/− mice. Visual acuity and ERG responses were decreased in Myrf+/− mice compared with the control mice (Myrf+/+). The loss of cone and rod neurons was proportional to the decreased outer nuclear layer (ONL) thickness. Moreover, RNA-seq revealed that phototransduction and estrogen signaling pathways played important roles in the Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis. Myrf+/− mouse strain provides a good model to investigate the function of the MYRF gene. Photoreceptor defects with impaired functions of spatial vision and retinal electrophysiology indicate an important role played by MYRF in retinal development. Alterations in phototransduction and estrogen signaling pathways play important roles in linking Myrf deficiency with retinal photoreceptor defects.
Real-world effectiveness of long-acting injectable antipsychotics to reduce 90-day and annual readmission in psychotic disorders: insights from a state psychiatric hospital
Authors: Patel Rikinkumar S. , Tankersley William E. 
Published: May 2021

AbstractBackgroundTo evaluate the effectiveness of long-acting injectable antipsychotics (LAI-a) in reducing the 90-day and annual readmission rates in schizophrenia inpatients.MethodsWe conducted a cross-sectional study and included 180 adult patients with psychotic disorders discharged from 2018 to 2019 at a state psychiatric hospital. Descriptive statistics were used to measure the differences between the readmit and nonreadmit cohorts. Logistic regression model was used to measure the odds ratio (OR) for 90-day and annual readmission and was controlled for potential readmission risk factors.ResultsA lower proportion of patients receiving LAI-a were readmitted within 90-day (28.6%) and 1-year (32.4%) periods. Patients receiving LAI-a had lower odds of association for 90-day (OR 0.36, 95% confidence intervals [CI] 0.139-0.921) and annual (OR 0.35, 95% CI 0.131-0.954) readmissions compared to those discharged on oral antipsychotics. A higher proportion of inpatients who received fluphenazine LAI had 90-day (25%) and annual (18.2%) readmissions compared to other LAI-a.ConclusionUtilization of LAI-a in patients with psychotic disorders can decrease both 90-day and annual psychiatric readmissions by 64% to 65%. Physicians should prefer LAI-a to reduce the readmission rate and improve the quality of life, and decrease the healthcare-related financial burden.
Cretaceous to Miocene NW Pacific Plate Kinematic Constraints: Paleomagnetism and Ar–Ar Geochronology in the Mineoka Ophiolite Mélange (Japan)
Authors: Ganbat Ariuntsetseg , Pastor‐Galán Daniel , Hirano Naoto , Nakamura Norihiro , Sumino Hirochika , Yamaguchi Yuji , Tsujimori Tatsuki 
Published: May 2021

AbstractThe Mineoka Ophiolite Mélange is located at the intersection of the Pacific, Philippine Sea, Eurasian, and North American plates. The Mineoka ophiolite origin is disputed, and it has been ascribed to a fully subducted plate or part of the Pacific and Philippine Sea plates. In this paper, we present a kinematic reconstruction of the Mineoka Ophiolite Mélange and its relation with the Pacific Plate, based on new paleomagnetic data and bulk‐rock 40Ar/39Ar ages of basaltic rocks. In addition to standard analyses for paleolatitudes, we performed a Net tectonic rotation analysis on sheeted dolerite dikes to infer the paleospreading direction that formed the ophiolite. The analysis shows that 85–80 Ma MOR pillow basalts erupted at a paleolatitude of N ∼16°, whereas ∼50 Ma basalts formed at N ∼34°. Net Tectonic Rotation analysis suggests that the spreading direction was NE 60°. Ar–Ar ages yielded 53–49 Ma for MORBs and 41–35 Ma for island‐arc basalts. The formation of this ophiolite occurred in the back‐arc spreading of the Nemuro–Olyutorsky arcs of the NW Pacific. It infers that the final consumption of Izanagi below Japan instigated a subduction jump and flipped its polarity. Subduction initiated parallel to the ridge, and a piece of the original back‐arc crust got trapped near the Japan trench during the northwards motion of the Philippine Sea Plate. The contrasting motion between the Pacific and the Philippine Sea plates generated a highly unstable setting followed by a subduction zone that left a small‐sized and short‐lived plate (“Mineoka”), surrounded by subduction zones.
Ice‐Shelf Meltwater Overturning in the Bellingshausen Sea
Authors: Ruan Xiaozhou , Speer Kevin G. , Thompson Andrew F. , Schulze Chretien Lena M. , Shoosmith Deborah R. 
Published: May 2021

AbstractHydrographic data are analyzed for the broad continental shelf of the Bellingshausen Sea, which is host to a number of rapidly thinning ice shelves. The flow of warm Circumpolar Deep Water (CDW) onto the continental shelf is observed in the two major glacially carved troughs, the Belgica and Latady troughs. Using ship‐based measurements of potential temperature, salinity, and dissolved oxygen, collected across several coast‐to‐coast transects over the Bellingshausen shelf in 2007, the velocity and circulation patterns are inferred based on geostrophic balance and further constrained by the tracer and mass budgets. Meltwater was observed at the surface and at intermediate depth toward the western side of the continental shelf, collocated with inferred outflows. The maximum conversion rate from the dense CDW to lighter water masses by mixing with glacial meltwater is estimated to be 0.37 ± 0.1 Sv in both depth and potential density spaces. This diapycnal overturning is comparable to previous estimates made in the neighboring Amundsen Sea, highlighting the overlooked importance of water mass modification and meltwater production associated with glacial melting in the Bellingshausen Sea.
“An Entrainment Paradox: How Hysteretic Saltation and Secondary Transport Augment Atmospheric Uptake of Aeolian Source Materials”
Authors: Rana Santosh , Anderson William , Day Mackenzie 
Published: May 2021

AbstractAerodynamic surface stress imposed by the atmospheric surface layer (ASL) drives aeolian sediment transport processes. When the imposed stress exceeds fluid threshold, splashing sand grains release fine (aerosol) particles. This process stops when the imposed stress falls below an impact threshold. Turbulence in the ASL is composed of elongated streaks of relatively high and low streamwise momentum (high‐momentum and low‐momentum regions, HMR and LMR, respectively), and these streaks are aligned with the prevailing winds. Streamwise‐wall‐normal turbulent stress is the concurrent product of fluctuations in streamwise and vertical velocity. These production mechanisms are categorized into quadrants: sweeps, inner interactions, ejections, and outer interactions, where the first two and last two occur within HMRs and LMRs, respectively. Under typical ASL conditions, the time‐averaged shear velocity is bound between 0.3 and 0.5 m/s, demonstrating the importance of fluctuations in mobilizing sand grains via saltation: time‐averaged shear velocity exceeding threshold does not correspond with typical ASL conditions. Given the aforementioned contributions to streamwise‐wall‐normal turbulent stresses from sweeps and ejections, it is self‐evident that under typical conditions only sweeps possess the momentum required to exceed threshold. But, any dust released is trapped by downwelling from aloft; ejections rarely exceed threshold, but they contain the positive vertical velocity needed to entrain. These attributes represent an entrainment paradox. Complementary field data are used to demonstrate the existence of an entrainment paradox. Large eddy simulation has been used to capture space‐time evolution of an idealized ASL and identify mechanistic flow physics central to entrainment.
Evaluation of Novel NASA Moderate Resolution Imaging Spectroradiometer and Visible Infrared Imaging Radiometer Suite Aerosol Products and Assessment of Smoke Height Boundary Layer Ratio During Extreme Smoke Events in the Western USA
Authors: Loría‐Salazar S. Marcela , Sayer Andrew M. , Barnes John , Huang Jingting , Flynn Connor , Lareau Neil , Lee Jaehwa , Lyapustin Alexei , Redemann Jens , Welton Ellsworth J. , Wilkins Joseph L. , Holmes Heather A. 
Published: May 2021

AbstractWe analyze new aerosol products from NASA satellite retrievals over the western USA during August 2013, with special attention to locally generated wildfire smoke and downwind plume structures. Aerosol optical depth (AOD) at 550 nm from MODerate Resolution Imaging Spectroradiometer (MODIS) (Terra and Aqua Collections 6 and 6.1) and Visible Infrared Imaging Radiometer Suite (VIIRS) Deep Blue (DB) and MODIS (Terra and Aqua) Multi‐Angle Implementation of Atmospheric Correction (MAIAC) retrievals are evaluated against ground‐based AErosol RObotic NETwork (AERONET) observations. We find a significant improvement in correlation with AERONET and other metrics in the latest DB AOD (MODIS C6.1 r2 = 0.75, VIIRS r2 = 0.79) compared to MODIS C6 (r2 = 0.62). In general, MAIAC (r2 = 0.84) and DB (MODIS C6.1 and VIIRS) present similar statistical evaluation metrics for the western USA and are useful tools to characterize aerosol loading associated with wildfire smoke. We also evaluate three novel NASA MODIS plume injection height (PIH) products, one from MAIAC and two from the Aerosol Single scattering albedo and layer Height Estimation (ASHE) (MODIS and VIIRS) algorithm. Both Terra and Aqua MAIAC PIHs statistically agree with ground‐based and satellite lidar observations near the fire source, as do ASHE, although the latter is sensitive to assumptions about aerosol absorption properties. We introduce a first‐order approximation Smoke Height Boundary Layer Ratio (SHBLR) to qualitatively distinguish between aerosol pollution within the planetary boundary layer and the free troposphere. We summarize the scope, limitations, and suggestions for scientific applications of surface level aerosol concentrations specific to wildfire emissions and smoke plumes using these novel NASA MODIS and VIIRS aerosol products.
Improving Convection Trigger Functions in Deep Convective Parameterization Schemes Using Machine Learning
Authors: Zhang Tao , Lin Wuyin , Vogelmann Andrew M. , Zhang Minghua , Xie Shaocheng , Qin Yi , Golaz Jean‐Christophe 
Published: May 2021

AbstractDeficiencies in convection trigger functions, used in deep convection parameterizations in General Circulation Models (GCMs), have critical impacts on climate simulations. A novel convection trigger function is developed using the machine learning (ML) classification model XGBoost. The large‐scale environmental information associated with convective events is obtained from the long‐term constrained variational analysis forcing data from the Atmospheric Radiation Measurement (ARM) program at its Southern Great Plains (SGP) and Manaus (MAO) sites representing, respectively, continental mid‐latitude and tropical convection. The ML trigger is separately trained and evaluated per site, and jointly trained and evaluated at both sites as a unified trigger. The performance of the ML trigger is compared with four convective trigger functions commonly used in GCMs: dilute convective available potential energy (CAPE), undilute CAPE, dilute dynamic CAPE (dCAPE), and undilute dCAPE. The ML trigger substantially outperforms the four CAPE‐based triggers in terms of theF1 score metric, widely used to estimate the performance of ML methods. The site‐specific ML trigger functions can achieve, respectively, 91% and 93%F1 scores at SGP and MAO. The unified trigger also has a 91%F1 score, with virtually no degradation from the site‐specific training, suggesting the potential of a global ML trigger function. The ML trigger alleviates a GCM deficiency regarding the overprediction of convection occurrence, offering a promising improvement to the simulation of the diurnal cycle of precipitation. Furthermore, to overcome the black box issue of the ML methods, insights derived from the ML model are discussed, which may be leveraged to improve traditional CAPE‐based triggers.
Remediating the Adverse Effects of Treated Wastewater Irrigation by Repeated On‐Surface Surfactant Application
Authors: Ogunmokun Felix A. , Wallach Rony 
Published: May 2021

AbstractThe use of treated wastewater (TWW) has gained recognition as an alternative source for freshwater irrigation, and is steadily expanding worldwide. Despite the benefits of freshwater conservation and nutrient richness, there is mounting evidence of TWW adverse effects on soil, yield, and the environment. Irrigation using TWW has resulted in soil water repellency, in which preferential flow pathways and uneven soil water and chemical distribution occur. These increase deep water percolation and chemical leaching, which can lead to soil and groundwater pollution. This study was conducted in a commercial citrus orchard grown on sandy‐loam soil in central Israel and irrigated with TWW, with the aim of investigating the remediation of these adverse effects, by repeatedly spraying a nonionic surfactant on the soil surface. The surfactant application succeeded to turn the soil wettable, diminishing the preferential flow pathways, and rendering the soil water and dissolved chemicals uniformly distributed. The overall water content in the 0–40 cm layer increased, and deep percolation and chemical leaching substantially decreased. The grapefruit yield increase during the two‐year study period increased the water use efficiency. Electrical resistance tomography scans executed during and after irrigation events for two subsequent years revealed that a “soil memory” phenomenon has been developed for water repellent soils, where water flow takes place through previously developed preferential flow pathways in such soils. This study demonstrates that recurrent surfactant application enables a continuous use of TWW, while eliminating most of its prejudicial effects.
Disentangling the Effects of Vapor Pressure Deficit and Soil Water Availability on Canopy Conductance in a Seasonal Tropical Forest During the 2015 El Niño Drought
Authors: Fang Yilin , Leung L. Ruby , Wolfe Brett T , Detto Matteo , Knox Ryan G , McDowell Nate G , Grossiord Charlotte , Xu Chonggang , Christoffersen Bradley O , Gentine Pierre , Koven Charles D , Chambers Jeffrey Q 
Published: May 2021

AbstractWater deficit in the atmosphere and soil are two key interactive factors that constrain transpiration and vegetation productivity. It is not clear which of these two factors is more important for the water and carbon flux response to drought stress in ecosystems. In this study, field data and numerical modeling were used to isolate their impact on evapotranspiration (ET) and gross primary productivity (GPP) at a tropical forest site in Barro Colorado Island (BCI), Panama, focusing on their response to the drought induced by the El Niño event of 2015–2016. Numerical simulations were performed using a plant hydrodynamic scheme (HYDRO) and a heuristic approach that ignores stomatal sensitivity to leaf water potential in the Energy Exascale Earth System Model (E3SM) Land Model (ELM). The sensitivity of canopy conductance (Gs) to vapor pressure deficit (VPD) obtained from eddy‐covariance fluxes and measured sap flux shows that, at both ecosystem and plant scale, soil water stress is more important in limiting Gs than VPD at BCI during the El Niño event. The model simulations confirmed the importance of water stress limitation on Gs, but overestimated the VPD impact on Gs compared to that estimated from the observations. We also found that the predicted soil moisture is less sensitive to the diversity of plant hydraulic traits than ET and GPP. During the dry season at BCI, seasonal ET, especially soil evaporation at VPD > 0.42 kPa, simulated using HYDRO and ELM, were too strong and will require alternative parameterizations.
Targeting autocrine amphiregulin robustly and reproducibly inhibits ovarian cancer in a syngeneic model: roles for wildtype p53
Authors: Lindzen Moshit , Ghosh Soma , Noronha Ashish , Drago Diana , Nataraj Nishanth Belugali , Leitner Orith , Carvalho Silvia , Zmora Einav , Sapoznik Stav , Shany Keren Bahar , Levanon Keren , Aderka Dan , Ramírez Belinda Sánchez , Dahlhoff Maik , McNeish Iain , Yarden Yosef 
Published: May 2021

AbstractOvarian cancer (OvCA) remains one of the most devastating malignancies, but treatment options are still limited. We report that amphiregulin (AREG) can serve as an effective and safe pharmacological target in a syngeneic murine model. AREG is highly abundant in abdominal fluids of patients with advanced OvCa. In immunocompetent animals, depletion or overexpression of AREG respectively prolonged or shortened animal survival. A new antibody we generated in AREG-knockout mice recognized murine AREG and reproducibly prolonged animal survival in the syngeneic model. The underlying mechanism likely involves binding of wildtype p53 to AREG’s promoter and autocrine activation of the epidermal growth factor receptor (EGFR), a step blocked by the antibody. Accordingly, depletion of p53 downregulated AREG secretion and conferred tolerance, whereas blocking an adaptive process involving CXCL1, which transactivates EGFR, might increase therapeutic efficacy. Consistent with these observations, analysis of OvCa patients revealed that high AREG correlates with poor prognosis of patients expressing wildtype TP53. In conclusion, clinical tests of the novel antibody are warranted; high AREG, normal TP53, and reduced CXCL1 activity might identify patients with OvCa who may derive therapeutic benefit.
Chlorine disinfection facilitates natural transformation through ROS-mediated oxidative stress
Authors: Zhang Shuai , Wang Yue , Lu Ji , Yu Zhigang , Song Hailiang , Bond Philip L , Guo Jianhua 
Published: May 2021

AbstractThe bacterial infection that involves antimicrobial resistance is a rising global threat to public health. Chlorine-based water disinfection processes can inactivate antibiotic resistant bacteria. However, at the same time, these processes may cause the release of antibiotic resistance genes into the water as free DNA, and consequently increase the risk to disseminate antibiotic resistance via natural transformation. Presently, little is known about the contribution of residual chlorine affecting the transformation of extracellular antibiotic resistance genes (ARGs). This study investigates whether chloramine and free chlorine promote the transformation of ARGs and how this may occur. We reveal that both chloramine and free chlorine, at practically relevant concentrations, significantly stimulated the transformation of plasmid-encoded ARGs by the recipient Acinetobacter baylyi ADP1, by up to a 10-fold increase. The underlying mechanisms underpinning the increased transformations were revealed. Disinfectant exposure induced a series of cell responses, including increased levels of reactive oxygen species (ROS), bacterial membrane damage, ROS-mediated DNA damage, and increased stress response. These effects thus culminated in the enhanced transformation of ARGs. This promoted transformation was observed when exposing disinfectant-pretreated A. baylyi to free plasmid. In contrast, after pretreating free plasmid with disinfectants, the transformation of ARGs decreased due to the damage of plasmid integrity. These findings provide important insight on the roles of disinfectants affecting the horizontal transfer of ARGs, which could be crucial in the management of antibiotic resistance in our water systems.
PP2Acα promotes macrophage accumulation and activation to exacerbate tubular cell death and kidney fibrosis through activating Rap1 and TNFα production
Authors: Liang Yan , Sun Xiaoli , Wang Mingjie , Lu Qingmiao , Gu Mengru , Zhou Lu , Hou Qing , Tan Mengzhu , Wang Sudan , Xue Xian , Dai Chunsun 
Published: May 2021

AbstractMacrophage accumulation and activation play an essential role in kidney fibrosis; however, the underlying mechanisms remain to be explored. By analyzing the kidney tissues from patients and animal models with kidney fibrosis, we found a large induction of PP2Acα in macrophages. We then generated a mouse model with inducible macrophage ablation of PP2Acα. The knockouts developed less renal fibrosis, macrophage accumulation, or tubular cell death after unilateral ureter obstruction or ischemic reperfusion injury compared to control littermates. In cultured macrophages, PP2Acα deficiency resulted in decreased cell motility by inhibiting Rap1 activity. Moreover, co-culture of PP2Acα−/−macrophages with tubular cells resulted in less tubular cell death attributed to downregulated Stat6-mediated tumor necrosis factor α (TNFα) production in macrophages. Together, this study demonstrates that PP2Acα promotes macrophage accumulation and activation, hence accelerates tubular cell death and kidney fibrosis through regulating Rap1 activation and TNFα production.