bluuebunny/crossref_metadata_2025_split huggingface dataset:
Handbook of Florida Agricultural Laws: Environmental and Conservation Regulations
Authors: Olexa Michael T. , Alperstein Ian , Fischer Joseph 
Published: November 2021

Revised! Circular 1224, a handbook broken into 8 parts, by Michael T. Olexa, Ian Alperstein, and Joseph Fischer, provides an accurate, current, and authoritative summary of the principal Florida laws that directly or indirectly relate to agriculture. It provides a basic overview of the many rights and responsibilities that farmers and farmland owners have under Florida laws. Published by the UF Department of Food and Resource Economics, December 2007.
FE118/FE118: Handbook of Florida Agricultural Laws: Environmental and Conservation Regulations (ufl.edu)
Ask IFAS: Handbook of Florida Agricultural Laws (Circular 1224) (ufl.edu)
La tortura en España: un ejemplo de turismo oscuro
Authors: Santamarina Otaola Josu 
Published: November 2021

Teniendo en cuenta que la sección de Debate tiene como eje central el turismo oscuro, este texto se presenta con el objetivo de reflexionar sobre un tema que tiene un particular reflejo en materia de turismo oscuro en España: la tortura. Existen decenas de museos sobre la tortura en este país y, sin embargo, éste sigue siendo un tema que está lejos de ser tratado como en los países de nuestro entorno.
Komik Digital Warak Ngendog Untuk Memperkenalkan Nilai Kearifan Lokal Kepada Anak Di Kota Semarang
Authors: Senoprabowo Abi , Khamadi Khamadi , Septian Yumna Anindya 
Published: November 2021

Upaya pelestarian budaya Warak Ngendog telah banyak dilakukan seperti identifikasi dan dokumentasi budaya tradisi lewat penulisan, foto dan video. Upaya tersebut hanya sampai kepada mereka yang benar-benar tertarik dengan budaya tradisi saja. Padahal saat ini budaya tradisi agar tetap lestari harus mampu mengikuti perkembangan zaman. Untuk dapat bertahan dengan baik, maka budaya Warak Ngendog harus bertransformasi menjadi lebih modern. Sehingga dengan memanfaatkan perkembangan IPTEK saat ini, Warak Ngendog dirasa dapat diadaptasikan ke dalam komik digital sehingga anak dapat memahami nilai kearifan lokal yang terkandung dalam Warak Ngendog. Penelitian ini menggunakan metode deskriptif kualitatif dengan pendekatan analisis data melalui nilai-nilai kearifan lokal dan pendekatan perancangan komik digital. Data yang dianalisis menjadi dasar perancangan komik berupa desain karakter komik dan unsur storytelling. Sebagai hasilnya, karakter komik berupa desain karakter Warak Ngendog dengan mengadaptasikan nilai estetika, religius, dan sosial dari nilai kearifan lokal yang dimilikinya. Komik Warak Ngendog mengedepankan nilai sosial dan religius dalam penceritaan dan dikembangkan dalam perancangan di media digital yang interaktif dan mudah diakses oleh anak saat ini.
Pengaruh Perceived Usefulness dan Perceived Ease of Use Terhadap Attitude Toward Using E-Wallet pada Mahasiswa Selama Pandemi COVID-19
Authors: Agustin Dwi Andriyanti , Wijaya Ryan Ariesco , Nugrahani Janviera Adriana 
Published: November 2021

Penelitian ini dilatarbelakangi oleh dampak pandemi COVID-19 yang meningkatkan transaksi di berbagai e-commerce. Metode pembayaran di berbagai e-commerce platform sudah jarang menggunakan uang tunai karena digantikan dengan menggunakan e-wallet untuk mengurangi kontak fisik. Penelitian ini menggunakan Technology Acceptance Model (TAM) sebagai landasan teori. Penelitian ini menguji pengaruh perceived usefulness dan perceived ease of use terhadap attitude toward using e-wallet pada mahasiswa selama pandemi COVID-19. Jenis penelitian yang digunakan adalah penelitian kausal. Populasi yang akan digunakan dalam penelitian ini adalah seluruh mahasiswa Universitas Ma Chung. Sedangkan untuk sampel yang digunakan adalah mahasiswa S1 Program Studi Manajemen dan mahasiswa S2 Program Magister Manajemen Inovasi Universitas Ma Chung dengan jumlah sebanyak 62 responden. Variabel dalam penelitian ini diukur dengan menggunakan skala Likert. Penelitian ini menggunakan uji validitas, uji reliabilitas, uji asumsi klasik, dan uji hipotesis. Berdasarkan hasil penelitian, perceived usefulness tidak berpengaruh terhadap attitude toward using mahasiswa dalam menggunakan e-wallet. Selain itu, perceived ease of use berpengaruh positif terhadap attitude toward using mahasiswa dalam menggunakan e-wallet.
This research is motivated by the impact of the COVID-19 pandemic which increased transactions in various e-commerce. Payment methods on various e-commerce platforms have rarely used cash because they use e-wallet to reduce physical contact. This study uses the Technology Acceptance Model (TAM) as the theoretical basis. This study examines the effect of perceived usefulness and perceived ease of use on students' attitudes towards using e-wallet during the COVID-19 pandemic. The type of research used is causal research. The population that used in this research was all students of Ma Chung University. Meanwhile, the samples used were students of the Bachelor of Management Studies Program and students of the Master's Degree Program in Innovation Management at Ma Chung University with a total of 62 respondents. The variables in this study were measured using a Likert scale. This research uses validity test, reliability test, classical assumption test, and hypothesis test. Based on the results of the study, perceived usefulness has no effect on student’s attitudes toward using e-wallet. In addition, the perception ease of use has a positive effect on student’s attitudes toward using e-wallet.
Analisis Postur Kerja Karyawan PT.XYZ Menggunakan Metode Ovako Work Posture Analysis Sistem (Owas)
Authors: Meri Mufrida , Linda Rozza , Rahayu Popi Gusti 
Published: November 2021

Tujuan dari penelitian ini adalah untuk menentukan kategori risiko sikap kerja karyawan pada PT.XYZ dengan menggunakan metode ovako work posture analysis system (OWAS). Berdasarkan hasil observasi awal terhadap sejumlah karyawan ternyata terdapat keluhan-keluhan yang dirasakan oleh pekerja pada anggota tubuh tertentu seperti leher, tulang punggung, pinggang, kaki dan bahu. Aktivitas kerja yang sering dilakukan oleh karyawan dilantai produksi PT.XYZ adalah gerakan membungkuk, jongkok, berdiri, selama satu hari kerja (8 jam) yang bisa menyebabkan keluhan musculoskeletal disorder (MSDs) yang merupakan penyakit atau gangguan pada jaringan lunak (otot, sendi, ligament, tendon, tulang rawan) dan sistem saraf. Dengan adanya keluhan – keluhan yang dirasakan karyawan maka penelitian ini menggunakan metode ovako work posture analysis system (OWAS) yang merupakan metode untuk menilai pembebanan pada postur tubuh saat bekerja. Penerapan dari metode ini dapat memberikan suatu hasil yang baik, yang dapat meningkatkan kenyamanan kerja, sebagai peningkatan kualitas produksi, setelah dilakukannya perbaikan sikap kerja. Dari penelitian ini didapat hasil kategori risiko yang paling banyak adalah kategori risiko no.2 yaitu sikap kerja ini berbahaya pada sistem musculoskeletal disorder ( sikap kerja mengakibatkan pengaruh ketegangan yang signifikan) perlu perbaikan segera mungkin.
Acclimation and Characterization of Marine Cyanobacterial Strains Euryhalinema and Desertifilum for C-Phycocyanin Production
Authors: Khazi Mahammed Ilyas , Li Chenshuo , Liaqat Fakhra , Malec Przemyslaw , Li Jian , Fu Pengcheng 
Published: November 2021

This study involves evaluation of two native cyanobacterial strains Euryhalinema and Desertifilum isolated from a mangrove pond in Haikou (China) for their possible phycocyanin (C-PC) production. Maximal growth rate with highest chlorophyll and C-PC accumulation were observed at 28°C and 60 μmol photons m−2 s−1 photon flux density for Euryhalinema sp., while for Desertifilum sp. at 32°C and 80 μmol photons m−2 s−1. Nitrogen and iron concentration trails revealed that double strength concentration of sodium nitrate and ferric ammonium citrate in original BG11 media increased growth rate and accumulation of C-PC for both strains. Three different C-PC extraction methods were tested. The combined extraction protocol of freeze–thaw and ultrasonication markedly increased the C-PC extraction efficiency and attained the food grade purity (A620/A280 ratio &gt;0.7), whereas a higher C-PC yield was found with Na-phosphate buffer. Furthermore, the clarified crude extract was used to purify C-PC by fractional ammonium sulfate [(NH₄)₂SO₄] precipitation, Sephadex G-25 gel filtration chromatography, and DEAE-sephadex ion exchange chromatography and attained analytical grade purity (A620/A280 ratio &gt;3.9). Taken together, both strains showed their potential to be domesticated for valuable phycocyanin production.
Analysis of Multi-Layer RNA Modification Patterns for the Characterization of Tumor Immune Microenvironment in Hepatocellular Carcinoma
Authors: Xing Jiyuan , Shen Shen , Dong Zihui , Hu Xiaobo , Xu Lixia , Liu Xiaorui , Li Qinggang , Zhang Yize , Cui Gangying , Yu Zujiang 
Published: November 2021

Background: RNA modifications have emerged as important posttranscriptional changes in multiple tumor cellular processes and tumorigenesis, including hepatocellular carcinoma (HCC). However, the potential roles and the interaction between regulators of RNA modifications and the tumor microenvironment (TME) are unclear in HCC.Methods: The gene expression profiles of 26 RNA modification “writers” were investigated in the TCGA cohort. The unsupervised clustering approach was used to class these RNA modification regulators. The characteristics of immune cell infiltration from TME for each cluster was tested by the CIBERSORT method. Additionally, we established a scoring model to evaluate the RNA modification characteristics of individual tumors. The associations between the scoring model and genetic as well as clinical characteristics, drug sensitivity, and response to immunotherapy were also analyzed.Results: We mapped the somatic mutations and somatic copy number variation of the RNA modification regulators. The expression of all selected regulators was detected, and two modification patterns were identified that featured distinct immune cell infiltration characteristics. Subsequently, we developed a score model (termed as WM-Score model). Furthermore, the survival analysis showed that the WM-Score value was associated with HCC patient prognosis. The results of the ROC curves analysis and multivariate analysis all confirmed that the WM-Score value was strongly associated with anti-cancer drug resistance and therapeutic efficacy of immunotherapy, thus could be used as an independent risk factor in HCC.Conclusion: Our research identified two RNA modification patterns characterized by distinct TME, and the WM-Score model was developed that might serve as reliable prognostic and immunotherapeutic effect predictor of HCC.
Immune Checkpoint Inhibitors With or Without Bone-Targeted Therapy in NSCLC Patients With Bone Metastases and Prognostic Significance of Neutrophil-to-Lymphocyte Ratio
Authors: Bongiovanni Alberto , Foca Flavia , Menis Jessica , Stucci Stefania Luigia , Artioli Fabrizio , Guadalupi Valentina , Forcignanò Maria Rosachiara , Fantini Manuela , Recine Federica , Mercatali Laura , Spadazzi Chiara , Burgio Marco Angelo , Fausti Valentina , Miserocchi Anna , Ibrahim Toni 
Published: November 2021

IntroductionBone metastases (BMs) are a negative prognostic factor in patients with non-small cell lung cancer (NSCLC). Although immune-checkpoint inhibitors (ICIs) have dramatically changed the therapeutic landscape of NSCLC, little information is available on BMs from NSCLC treated with ICIs alone or in association with bone-targeted therapy (BTT) such as zoledronate or denosumab.MethodsFrom 2014 to 2020, 111 of the 142 patients with BMs secondary to NSCLC extrapolated from the prospective multicenter Italian BM Database were eligible for analysis. Information on blood count, comorbidities, and toxicity was retrospectively collected. The neutrophil-to-lymphocyte ratio (NLR) pre- and post-treatment was calculated. Survival was analyzed using the Kaplan–Meier method, with statistical significance of survival differences assessed using the log-rank test.ResultsMedian age was 66 (range, 42–84) years. Performance status (PS) Eastern Cooperative Oncology Group (ECOG) was 0–1 in 79/111 patients. The majority of patients (89.2%) had adenocarcinoma histology. At a median follow-up of 47.4 months, median progression-free (mPFS) and overall survival (mOS) was 4.9 (95%CI, 2.8–10.0) and 11.9 (95%CI, 8.2–14.4) months, respectively. Forty-six (43.4%) patients with BM NSCLC underwent first- or further-line therapy with ICIs: 28 (60.8%) received nivolumab, 9 (19.6%) pembrolizumab, and 9 (19.6%) atezolizumab. Of the 46 patients treated with ICIs, 30 (65.2%) underwent BTT: 24 (80.0%) with zoledronate and 6 (20.0%) with denosumab. The ICI-alone group had an mOS of 15.8 months [95%CI, 8.2–not evaluable (NE)] vs. 21.8 months (95%CI, 14.5–not evaluable) for the ICI plus BTT group and 7.5 (95%CI, 6.1–10.9) months for the group receiving other treatments (p &lt; 0.001). NLR ≤5 had a positive impact on OS.ConclusionBTT appears to have a synergistic effect when used in combination with ICIs, improving patient survival.
Ellipsoid Zone and External Limiting Membrane-Related Parameters on Spectral Domain-Optical Coherence Tomography and Their Relationships With Visual Prognosis After Successful Macular Hole Surgery
Authors: Yang Jiarui , Xia Huaqin , Liu Yushi , Wang Xinglin , Yuan Hao , Hou Qingyi , Ge Yimeng , Ding Yi , Wang Yuexin , Wang Changguan , Li Xuemin 
Published: November 2021

Purpose: To compare structural diameters of the ellipsoid zone (EZ) and external limiting membrane (ELM) bands on spectral domain-optical coherence tomography (SD-OCT) images between vision-improved (group A) and vision-unimproved (group B) patients, and investigate the connection between these parameters and visual prognosis.Materials and Methods: Forty-five eyes of 43 patients with idiopathic full-thickness macular hole closed after vitrectomy were retrospectively reviewed. Best-corrected visual acuity (BCVA) and SD-OCT were conducted preoperatively and at 1 week, 1 month and 6 months postoperatively. Structural and functional parameters were then measured using ImageJ software.Results: Among structural and functional parameters, the relative reflectivity of EZ and the ratio of continuous ELM and EZ in group A were significantly higher than in group B from the 1-month postoperative visit. At the 6-month follow-up, the diameter of EZ disruption in group A was significantly smaller than in group B, and the relative reflectivity of ELM/EZ was significantly higher than group B. At 6-months, BCVA was statistically significantly correlated with baseline BCVA, basal diameter (BD), macular hole index (MHI), and diameter of ELM/EZ disruption. Change in BCVA from baseline was found to be significantly correlated with axial length and diameter hole index (DHI).Conclusions: Postoperative BCVA outcome was significantly correlated with integrity, thickness and reflectivity of the EZ band. Patients with smaller diameter of EZ disruption and higher reflectivity of EZ band tended to have better visual outcomes. Given that the EZ band reflects the recovery of mitochondria in photoreceptors, it is a promising parameter for their functional evaluation.
Standardized Diagnostic Workup and Patient-Centered Decision Making for Surgery and Neck Dissection Followed by Risk-Factor Adapted Adjuvant Therapy Improve Loco-Regional Control in Local Advanced Oral Squamous Cell Carcinoma
Authors: Wichmann Gunnar , Pavlychenko Mykola , Willner Maria , Halama Dirk , Kuhnt Thomas , Kluge Regine , Gradistanac Tanja , Fest Sandra , Wald Theresa , Lethaus Bernd , Dietz Andreas , Wiegand Susanne , Zebralla Veit 
Published: November 2021

BackgroundStandardized staging procedures and presentation of oral squamous cell carcinoma (OSCC) patients in multidisciplinary tumor boards (MDTB) before treatment and utilization of elective neck dissection (ND) are expected to improve the outcome, especially in local advanced LAOSCC (UICC stages III–IVB). As standardized diagnostics but also increased heterogeneity in treatment applied so far have not been demonstrated to improve outcome in LAOSCC, a retrospective study was initiated.MethodsAs MDTB was introduced into clinical routine in 2007, 316 LAOSCC patients treated during 1991-2017 in our hospital were stratified into cohort 1 treated before (n=104) and cohort 2 since 2007 (n=212). Clinical characteristics, diagnostic procedures and treatment modality of patients were compared using Chi-square tests and outcome analyzed applying Kaplan-Meier plots and log-rank tests as well as Cox proportional hazard regression. Propensity scores (PS) were used to elucidate predictors for impaired distant metastasis-free survival (DMFS) in PS-matched patients.ResultsMost patient characteristics and treatment modalities applied showed insignificant alteration. Surgical treatment included significantly more often resection of the primary tumor plus neck dissection, tracheostomy and percutaneous endoscopic gastrostomy tube use. Cisplatin-based chemo-radiotherapy was the most frequent. Only insignificant improved disease- (DFS), progression- (PFS) and event-free (EFS) as well as tumor-specific (TSS) and overall survival (OS) were found after 2006 as local (LC) and loco-regional control (LRC) were significantly improved but DMFS significantly impaired. Cox regression applied to PS-matched patients elucidated N3, belonging to cohort 2 and cisplatin-based chemo-radiotherapy as independent predictors for shortened DMFS. The along chemo-radiotherapy increased dexamethasone use in cohort 2 correlates with increased DM.ConclusionsDespite standardized diagnostic procedures, decision-making considering clear indications and improved therapy algorithms leading to improved LC and LRC, shortened DMFS hypothetically linked to increased dexamethasone use had a detrimental effect on TSS and OS.
The Identification of a Tumor Infiltration CD8+ T-Cell Gene Signature That Can Potentially Improve the Prognosis and Prediction of Immunization Responses in Papillary Renal Cell Carcinoma
Authors: Wang Jie , Huang Meiying , Huang Peng , Zhao Jingjie , Tan Junhua , Huang Feifan , Ma Ruiying , Xiao Yu , Deng Gao , Wei Liuzhi , Wei Qiuju , Wang Zechen , He Siyuan , Shen Jiajia , Sooranna Suren , Meng Lingzhang , Song Jian 
Published: November 2021

BackgroundCD8+ T cells, vital effectors pertaining to adaptive immunity, display close relationships to the immunization responses to kill tumor cells. Understanding the effect exerted by tumor infiltration CD8+ T cells in papillary renal cell carcinoma (papRCC) is critical for assessing the prognosis process and responses to immunization therapy in cases with this disease.Materials and ApproachesThe single-cell transcriptome data of papRCC were used for screening CD8+ T-cell-correlated differentially expressed genes to achieve the following investigations. On that basis, a prognosis gene signature associated with tumor infiltration CD8+ T cell was built and verified with The Cancer Genome Atlas data set. Risk scores were determined for papRCC cases and categorized as high- or low-risk groups. The prognosis significance for risk scores was assessed with multiple-variate Cox investigation and Kaplan–Meier survival curves. In addition, the possible capability exhibited by the genetic profiles of cases to assess the response to immunization therapy was further explored.ResultsSix hundred twenty-one cell death-inhibiting RNA genes were screened using single-cell RNA sequencing. A gene signature consisting of seven genes (LYAR, YBX1, PNRC1, TCF25, MYL12B, MINOS1, and LINC01420) was then identified, and this collective was considered to be an independent prognosis indicator that could strongly assess overall survival in papRCC. In addition, the data allowed papRCC cases to fall to cohorts at high and low risks, exhibiting a wide range of clinically related features as well as different CD8+ T-cell immunization infiltration and immunization therapy responses.ConclusionsOur work provides a possible explanation for the limited response of current immunization checkpoint-inhibiting elements for combating papRCC. Furthermore, the researchers built a novel genetic signature that was able to assess the prognosis and immunotherapeutic response of cases. This may also be considered as a promising therapeutic target for the disease.
Risk Factors for Severe Hand-Foot-Mouth Disease in China: A Systematic Review and Meta-Analysis
Authors: Li Peiqing , Huang Yuge , Zhu Danping , Yang Sida , Hu Dandan 
Published: November 2021

Background: This study aimed to identify potential risk factors for severe hand-foot-mouth disease (HFMD).Methods: The PubMed, Embase, the Cochrane Library, Sinomed, WanFang, CNKI, and VIP databases were searched (up to August 2021).Results: Twenty-nine studies (9,241 and 927,355 patients with severe HFMD and controls, respectively; all from China) were included. EV71 was associated with higher odds of severe HFMD compared with other agents (OR = 4.44, 95%CI: 3.12–6.33, p &lt; 0.001). Being home-raised (OR = 1.99, 95%CI: 1.59–2.50, p &lt; 0.001), higher number of children in the family (OR = 2.09, 95%CI: 1.93–2.27, p &lt; 0.001), poor hand hygiene (OR = 2.74, 95%CI: 1.78–4.23, p &lt; 0.001), and no breastfeeding (OR = 2.01, 95%CI: 1.45–2.79, p &lt; 0.001) were risk factors for severe HFMD. First consulting to a district-level or above hospital (OR = 0.34, 95%CI: 0.25–0.45, p &lt; 0.001) and diagnosis of HFMD at baseline (OR = 0.17, 95%CI: 0.13–0.24, p &lt; 0.001) were protective factors against severe HFMD. Fever, long fever duration, vomiting, lethargy, leukocytosis, tic, and convulsions were each associated with severe HFMD (all p &lt; 0.05), while rash was not.Conclusions: EV71, lifestyle habits, frequent hospital visits, and symptoms are risk factors for severe HFMD in children in China, while early diagnosis and admission to higher-level hospitals are protective factors.
Analysis of the Mediating Effect of Risk Perception on the Relationship Between Time Perception and Mental Health of College Students During the COVID-19 Epidemic
Authors: Cao Hua , Zhang Hui Wen , Yang Ling , Li Ling , Wang Jun Zhi , Zada Bakht , Li Min Xiao , Liu Wei Jian , Su Ting Hong , Zhao Yu 
Published: November 2021

Background: COVID-19 has had a wide impact on the mental health of college students. This study aims to explore the relationship between time perception, risk perception, and the mental health of college students during COVID-19 through a questionnaire survey.Subjects: One thousand two hundred and eighteen college students, 449 male and 769 female, who studied online during the COVID-19 epidemic were selected.Methods: Time Perception Scale, Risk Perception Scale, and SCL-90 were used to investigate the relationship using correlation analysis.Results: During the COVID-19 period, mental health problems of college students were widespread, and 65.93% of college students reported moderate to severe mental health problems. The correlation analysis showed that risk perception, time perception, and the mental health of college students were significantly related. Risk perception played a partial mediating role between present enjoyment and mental health, and risk perception played a partial mediating role between future time perception and mental health.Conclusion: In the case of sudden public crises, we should pay close attention to the mental health of college students, adjust their attitude toward the present and the future, and pay attention to their perception of risk so as to improve their mental health level under crisis.