bluuebunny/crossref_metadata_2025_split huggingface dataset:
Development and Validation of a Program Logic Model (PLM) to Support "Near Miss Mother"(NMM): A Nominal Group Technique (NGT)
Authors: Abdollahpour Sedigheh , Heydari Abbas , Ebrahimipour Hosein , Faridhoseini Farhad , Khadivzadeh Talat 
Published: February 2021

Abstract
Background: Mothers who have experienced a near miss event, their normal life is affected by physical, psychological, emotional, social and economic adverse effects. The aim of this study is to develop a supportive program for near miss mothers (NMM), based on a program logical model (PLM) that has been validated using the nominal group technique (NGT).Methods: After conducting qualitative and systematic reviews studies to assess the needs, components of PLM were extracted that provided the framework for the utilization of activities, outputs, outcomes and impact. A Nominal Group Technique method done in a one-day workshop with the participation of 12 professionals was held in November 2020.Results: Eight strategies used in draft support programs based on the logical model, included the following: "psychological", "fertility / childbearing", "information", "care quality improvement", "socio-cultural", "financial", "breastfeeding" and "nutritional". The validation of the program was done based on the five steps of the NGT during the steps of creating ideas, silent generation of ideas, round robin, clarification of ideas, Prioritization. Finally, a final program was presented to support NMM.Conclusions: Simultaneous integration in the PLM and NGT method allowed the first program developed to support NMM to be comprehensive and complete. Using this evidence-based program can help reduce the burden of maternal morbidities in millions of women around the world and prevent long-term complications and shorten their rehabilitation phase.
Mutation and Microsatellite Burden Predict Response to PD-1 Inhibition in Children with Germline DNA Replication Repair Deficiency
Authors: Tabori Uri , Morgenstern Daniel , Sudhaman Sumedha , Das Anirban , Coblentz Ailish , Chung Jiil , Stone Simone , Alsafwani Noor , Cabric Vanja , Nobre Liana , Bianchi Vanessa , Edwards Melissa , Sambira Lauren , Constantini Shlomi , Dvir Rina , Yalon Michal , Campino Gadi , Caspi Shani , Larouche Valerie , Reddy Alyssa , Osborn Michael , Mason Gary , Lindhorst Scott , Bronsema Annika , Magimairajan Vanan , Opocher Enrico , De Mola Rebecca , Sabel Magnus , Frojd Charlotta , Sumerauer David , Samuel David , Cole Kristina , Chiaravalli Stefano , Massimino Maura , Tomboc Patrick , Ziegler David , George Ben , Damme An Van , Hijaya Nobuko , Gass David , McGee Rose , Mordechai Oz , Bowers Daniel , Laetsch Ted , Lossos Alexander , Blumenthal Deborah , Sarosiek Tomasz , Yen Lee , Knipstein Jeffrey , Bendel Anne , Hoffman Lindsey , Luna-Fineman Sandra , Zimmermann Stefanie , Scheers Isabelle , Nichols Kim , Zapotocky Michal , Hansford Jordan , Maris John , Dirks Peter , Taylor Michael , Kulkarni Abhaya , Shroff Manohar , Tsang Derek , Malkin David , Villani Anita , Aronson Melyssa , Durno Carol , Shlien Adam , Getz Gad , Maruvka Yosef , Ohashi Pamela , Hawkins Cynthia , Pugh Trevor , Bouffet Eric 
Published: February 2021

Abstract
Cancers arising from germline DNA mismatch-repair or polymerase-proofreading deficiencies (MMRD and PPD) in children harbour the highest mutational and microsatellite insertion/deletion (MS-indel) burden in humans and are lethal due to inherent resistance to chemo-irradiation. Although immune checkpoint inhibitors (ICI) have failed to benefit children in previous studies, we hypothesized that hypermutation caused by MMRD and PPD will improve outcomes following ICI in these patients. ICI treatment of 45 progressive/recurrent tumours from 38 patients revealed durable objective responses in the majority, culminating in 3-year survival of 41.4%. High mutation burden predicted response for ultra-hypermutant cancers (>100 mutations/Mb) enriched for combined MMRD+PPD, while MS-indels predicted response in MMRD tumours with lower mutation burden (10-100 mutations/Mb). Further, both mechanisms were associated with increased immune infiltration even in “immunologically-cold” tumours such as gliomas, contributing to the favorable response. Pseudo-progression (flare) was common and associated with immune activation in both the tumour microenvironment and systemically. Further, patients with flare continuing ICI treatment achieved durable responses. Our study demonstrates improved survival for patients with tumours not previously known to respond to ICI, including CNS and synchronous cancers, and identifies the dual roles of mutation burden and MS-indels in predicting sustained responses to immunotherapy.
Frequency of Tooth Brushing as a Predictive Factor for Future Kidney Function Decline
Authors: Hirano Keita , Shimbo Takuro , Komatsu Yasuhiro , Kobayashi Daiki 
Published: February 2021

Abstract
BackgroundTooth brushing is important for maintaining oral health and preventing periodontal diseases, which commonly arise in patients with chronic kidney disease (CKD). However, the association between tooth brushing frequency and kidney function decline remains unclear. MethodsWe conducted a retrospective longitudinal study at St Luke’s International Hospital, Japan, and participants who underwent health examinations at the Centre for Preventive Medicine from 2005 to 2011 were included. Participants’ tooth brushing frequencies were assessed; multivariate analyses were conducted using a generalized estimating equation to evaluate the association between tooth brushing frequency and a composite renal outcome – composed of a 25% eGFR reduction, an eGFR of <15 mL/min/1.73 m², and a requirement for regular dialysis – after adjusting for potential covariates. We also stratified participants by baseline CKD risk category to perform sub-analyses. ResultsOverall, 76,472 participants were included (mean age of 45.9 years) and 38,233 (50%) were male. During follow-up, 8,219 participants (10.8%) experienced composite renal outcomes. Brushing teeth at least once to twice a day was associated with significantly lower incidences of composite renal outcomes than brushing teeth less frequently (adjusted odds ratio [OR]: 0.26; 95% confidence interval [CI]: 0.24–0.28 for once to twice a day; adjusted OR: 0.65; 95% CI: 0.62–0.69 for after every meal). In our sub-analyses, brushing at least once to twice a day related to a decreased likelihood of composite renal outcomes; however, this effect was only observed within the low and moderate baseline risk groups. ConclusionFrequent tooth brushing benefits oral health and may prevent kidney function decline, which could have implications for other systemic diseases. However, a longitudinal cohort study is required to confirm whether tooth brushing can prevent poor renal outcomes.
Parental Health Risk Perceptions and Preventive Measures on Children’s Exposure to Secondhand Smoke (SHS) of Tobacco Cigarette in Malaysia: A Nationwide Study
Authors: JUNUS Suria , CHEW Chii-Chii , SUGUNAN Pravin , MEOR-AZIZ Nurul-Fazlin , ZAINAL Nurul Ain , HASSAN Husna Mat , ABU-MANSOR Mazetty-Aiza , ABU-ZAMRI Hazirah , HSS Amar-Singh 
Published: February 2021

Abstract
Background: Secondhand smoke (SHS) exposure can affect physical development in children. An understanding of parental risk perception of SHS aid in developing protectives measures to prevent children from SHS exposure. This study aimed to assess parental risk perceptions of SHS and action taken by parents in minimising their children from exposing to SHS.Methods: This cross-sectional nationwide study conducted in 2018 conveniently recruited 289 parents with children up to age 12 at the public areas. Parents were asked to rate the risk level from 1 (no risk) to 5 (extremely high risk) by looking at the photographs of an adult smoked in the presence of a child in 8 different situations. The implementation of smoking restriction rules was assessed. Mean score was calculated with higher scores represent higher risk of SHS to child’s health. Linear regression analysis was used to determine factors associated with the level of parental risk perception of SHS exposure to the children’s health.Results: A total of 246 parents responded. Their mean age was 35 years old (SD 6.4), majority were mothers (75.6%), Malays (72.0%), had tertiary education level (82.5%), and had a youngest child with mean age of 3 years (SD 3.1). The risk perception level was high [mean scores: 4.11 (SD: 0.82)]. Most parents implemented household (65.0%) and car (68.3%) smoking restriction rules. Negative risk perception level was observed among participants who were fathers with lower occupation class (p=0.005), lived with a smoker (p=0.023), allowed household smoking with open window (p=0.001), being a father and lived with a smoker (p=0.020), and mothers with managerial position while the fathers had a lower occupation class (p=0.038).Conclusion: Most parents perceived that risks of SHS exposure to their children was high but only two-thirds of them had set smoking prohibiting rules. Health policymaker should pay attention to tactors associated with negative risk perceptions among parents.Trial registration: This study is approved by Medical Research Ethics Committee, Ministry of Health Malaysia (Registration Number: NMRR-18-3299-44967).
IL-21 Rescues The Defect of IL-10-Producing Regulatory B Cells and Improves Allergic Asthma in DOCK8 Deficient Mice
Authors: Jiang Jinqiu , Qin Tao , Zhang Liang , Liu Qiao , Wu Jiabin , Dai Rongxin , Zhou Lina , Zhao Qin , Luo Xiaoyan , Wang Hua , Zhao Xiaodong 
Published: February 2021

Abstract
Purpose Mutations in human DOCK8 cause a combined immunodeficiency syndrome characterized by allergic diseases such as asthma and food allergy. However, the underlying mechanism is unclear. Regulatory B (Breg) cells that produce IL-10 exert potent immunosuppressive functions in patients with allergic and autoimmune disorders. DOCK8-deficient B cells have been revealed diminished responses to LPS stimulation, suggesting a possible defect in IL-10-producing Breg cells in those with DOCK8 deficiency, which may contribute to allergies.Methods we collected peripheral blood mononuclear cells from DOCK8-deficient patients and generated a DOCK8KO mouse model to study the effect of DOCK8 deficiency on the Regulatory B cells.Results We show that DOCK8-deficient patients and DOCK8KO mice harbor quantitative and qualitative defects in IL-10-producing Breg cells; these defects are caused by abnormal DOCK8-/- IL-21-producing CD4+ T cells. We found that recombinant murine (rm)IL-21 restores the function of Bregs both in vitro and in DOCK8KO mice, leading to reduced inflammatory cell infiltration of the lungs in a murine asthma model. Conclusion Overall, the results provide new insight into the potential design of Breg-based or IL-21-based therapeutic strategies for allergic diseases, including asthma with DOCK8 deficiency.
Thermal dynamics and electronic temperature waves in layered correlated materials
Authors: Mazza Giacomo , Gandolfi Marco , Capone Massimo , Banfi Francesco , Giannetti Claudio 
Published: February 2021

Abstract
We explore layered strongly correlated materials as a platform to identify and control
unconventional heat transfer phenomena.
We demonstrate that these systems can be tailored to sustain a wide spectrum of heat transport regimes, ranging from ballistic, to hydrodynamic all the way to diffusive. Within the hydrodynamic regime, wave-like temperature oscillations are predicted up to room temperature. Temperature waves have a purely electronic origin, stemming from the existence of two components in the electronic system, each one thermalized at different temperatures. The interaction strength can be exploited as a knob to control the dynamics of temperature waves as well as the onset of different thermal transport regimes.
The present results pave the way to transition-metal oxide heterostructures as building blocks for nanodevices exploiting the wave-like nature of heat transfer on the picosecond time scale.
Blockchain Based Smart Healthcare Systems in 5G Networks For Preventing Data Forgery
Authors: helen suji , Senthilsingh C. 
Published: February 2021

Abstract
The 5G networks are about to deploy it all over the world. This 5G technologies support by connecting the devices with rapid growth in network capacity, high QoS. Apart from this feature, 5G has more advantages in security, decentralization, transparency, data interoperability. The 5G network has millions of IoT devices are connected. With higher speeds these devices are enabled and worked with high speed. Blockchain is an important technology in the current trend. The Blockchain technology is used in more fields such as online payments, healthcare, smart contracts etc. Extending the technology of block chain to Internet of things (IoT) can have more features. The important issues in 5G technology is security because millions of IoT devices are connected and more confidential data is transferred. This data should be more secure using blockchain technology. This proposed system is to secure the data in smart healthcare systems using blockchain in 5G networks to prevent the data from forgery.
Research on the Physical Properties of Calcium Sulfate Whisker and the Effects of its Addition on Paper and its Printing Performance
Authors: Liu Haitang , Liu Jing , An Yongzhen , Li Jie , Li Xuexiu , Jin Xin , Chen Lin , Gao Yujie , Lu Zhenhua , Si Chuanling 
Published: February 2021

Abstract
In this investigation, softwood pulp and bleached wheat straw pulp were used as raw materials, and Calcium Sulfate Whiskers (CSW) were used as fillers. Based on the structural properties and characteristics of CSW, the printing properties of the paper with CSW, such as whiteness, opacity, pH value, uniformity, Z-direction tensile strength and surface strength, were analyzed. The results showed that CSW has low solubility, high retention rate and fiber-like appearance. The whiteness of the paper is the best when the filling amount of CSW is 20%. The paper opacity of softwood pulp increases significantly, and the opacity of bleached wheat straw pulp decreases significantly when CSW is more than 30%. The addition reduces pH and surface strength of the paper. The evenness of the paper does not change obviously. The Z-direction tensile strength of the paper decreases in the softwood paper, but increases obviously in the bleached wheat straw paper. Graphic abstract
Effectiveness of the Caregiver-Mediated Intervention: A Pilot Study for Children with Neurodevelopmental Disorders
Authors: Ge Dongmei , Wei Hua , Wang Yue , Li Yan , Luo Jinmei , Liu Xiao , Hu Yan , Chen Li , Cheng Qian , Dai Ying 
Published: February 2021

Abstract
Background: The Caregiver-Mediated Intervention(CMI), based on Parent Skills Training(PST), is one family-mediated intervention model for children with neurodevelopmental disorders(NDDs), especially for ASD. This study aims to evaluate the effectiveness of CMI. Methods: 33 children (aged 22-69 months from our department) and their caregivers were included in a two-week training of ten 90-minute lessons. Then caregivers were encouraged to try their best to apply intervention skills in both home routines and play routines, in order to prompt the ability of cognition, motion, social adaptability and behavior of children. The based demographic information, video-taking data and diagnostic sales were collected at two key time‐points at least: Baseline(BL); Post-training (PT; within six months). Results: The outcomes mainly involved three aspects - primary variables, secondary variables and correlation analyses. Compared with BL, those favored PT in (1) Adult/Child Interaction Fidelity Rating (P<0.01), (2) adaptability of Gesell Developmental Scale and stereotyped behaviors and limited interests of Autism Diagnostic Observation Schedule (P<0.05, P<0.01). Moreover, a negative correlation appeared between caregiver skill improvement and parent education(P<0.05), without correlation with other demographics. Conclusions: As an efficacious family intervention for both children and their caregivers, CMI, with little limitation, is worth being generalized widely. Trial registration: China Clinical Trial Registry (ChiCTR-IOC-17013638, 15/12/2017).
Dissecting Four Correlated Growth Period Traits Using a Genome-Wide Association Study Approach in Soybean
Authors: Li Yinghui , Yanfei Li , Huilong Hong , Yansong Ma , Yongzhe Gu , Zhangxiong Liu , Scott Jackson , Lijuan Qiu 
Published: February 2021

Abstract
Soybean (Glycine max (L.) Merri) is a short-day, photoperiod-sensitive crop. The vegetative growth period (V), reproductive growth period (R), whole growth period (V + R) and the ratio of growth period structure (R/V) are photoperiod traits critical to adaptability and yield in soybean. To thoroughly dissect the genetic basis of these growth period traits and their correlations, a panel of 277 soybean accessions were genotyped with a single nucleotide polymorphisms (SNPs) chip and phenotyped at three field environments over three years. A genome-wide association study found 29 SNPs on fifteen chromosomes with 33 significant marker-trait associations, eleven, eight, four and ten for V, R, V + R and R/V, respectively. Two SNPs, Map-6077 and − 6502, showed pleiotropy in three and two traits, respectively. Map-6077, associated with V, R, and V + R, was a synonymous SNP of the GmMFT, a member of PEBP family that has been reported to inhibit seed germination and regulate oil content. These results provide candidate SNP markers and genes for further functional experiments to advance molecular marker-assisted breeding.
Effectiveness and Efficiency of Monosodium Glutamate as a Potential Mutagen Inducing Polyploidy in Urginea Indica Kunth
Authors: SINHA RICHA 
Published: February 2021

Abstract
Monosodium Glutamate is a food additive well-known as a flavor enhancer being used extensively in food industries. Also, it has long been used as a cheap source of fertilizer owing to the presence of high nitrogen contents in it. Furthermore, it also shows genotoxic effects on plants. Hence, in the present study the genotoxic properties of Monosodium Glutamate had been explored to assess its potential as a mutagen. The objective was specifically to find out its potential in inducing polyploidy in plants. For this, a medicinal plant, Urginea indica Kunth with immensely high therapeutic value was selected. The effectiveness and efficiency of Monosodium Glutamate on Urginea indica Kunth was assessed based on the records obtained after treatments in two generations, viz. M1 and M2 Generations. Its effectiveness and efficiency on Urginea indica Kunth pave a way for its use as a potential mutagen which can induce genetic variability among the selected wild species of the plants with great ease. Moreover, the higher degree of polyploidy obtained in the M2 generation established the objective of the experiment, that Monosodium Glutamate could be used with controlled, adequate dose and maintained experiment setup in inducing polyploidy in plants.
Rediscovery and Characterization of Citrus Indica Tanaka, A Wild Endangered and Progenitor Species in Dailong Forest, Manipur, and Recommendations for Its Conservation.
Authors: Devi Elangbam Julia , Labala Rajendra Kumar , Sanabam Rakesh , Singh Nandeibam Samarjit , Modak Rahul , Devi Huidrom Sunitibala 
Published: February 2021

Abstract
Citrus indica Tanaka is a wild endangered and one of the progenitor species of citrus endemic to North East (NE) India, which is reported in Nokrek Biosphere Reserve in the Garo hills of Meghalaya. A wild orange morphologically claimed to be C. indica was encountered in the Dailong forest of Tamenglong district, Manipur which is located in the Indo-Burma biodiversity hotspot region. Surveys were conducted to establish the exact identity of possible C. indica and study the natural habitat in Dailong forest area. The traditional morpho-taxonomic description for the identification of the plant is performed along with the molecular characterization using three chloroplast regions (trnL-F, psbK-I, matK-5′trnK spacer) and one nuclear (ITS) region. Phylogenetic analysis revealed that the C. indica found in Dailong Forest is monophyletic with the earlier reported C. indica sequence from Garo hills, Meghalaya. Both morphological and molecular data support the identity of C. indica that has been collected from Manipur. However, lack of knowledge and clearing of forest cover has led to an urgent need for the implementation of conservation strategies to safeguard this endangered species. This study supports the evidence that NE India is one of the origin centres for citrus and this finding can guide the conservationists to initiate priority for adopting possible methods of proper conservation of C. indica in the region.
Inhibitory effects of aviptadil on the SARS-CoV-2 nsp10/ nsp16 protein complex
Authors: Alnomasy Sultan F. , Alotaibi Bader S. , Aldosari Ziyad M. , Mujamammi Ahmed H. , Anand Pragya , Akhter Yusuf Akhter , Hasan Mohammad R. 
Published: February 2021

Abstract
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which emerged in late 2019, causes COVID-19, a disease that has been spreading rapidly worldwide. In human lung epithelial cells and monocytes, RLF-100 (aviptadil) has been found to inhibit the RNA replication machinery of SARS-CoV-2, which includes several non-structural proteins (nsp) that play essential roles in synthesizing and replicating viral RNA. This virus is unique in requiring nsp10 and nsp16 for methyltransferase (MTase) activity. This enzyme is essential for RNA stability, protein translation, and viral ability to escape the host's immune recognition. Therefore, we aimed to use bioinformatics tools to analyze aviptadil's inhibitory effect on the SARS-CoV-2 nsp10/nsp16 complex. We present a comprehensive, in silico-generated picture showing how aviptadil may interact with the nsp complex. Specifically, our model predicts how the initial binding of aviptadil to nsp10 and nsp16 may occur. This knowledge can assist drug development efforts against SARS-CoV-2 by providing more target information against nsp16.
sFLT01 Modulates Invasion and Metastasis in Prostate Cancer DU145 Cells By Inhibition of VEGF/GRP78/MMP2&amp;9 Axis
Authors: Taghizadeh Sepideh , Soheili Zahra-Soheila , Sadeghi Mehdi , Samiei shahram , Zakeri Fahimeh , Pirmardan Ehsan Ranaei , kashanian Ali , Najafabadi Hoda Shams 
Published: February 2021

Abstract
BackgroundAbout 90% of cancer-related deaths are due to metastasis of cancer cells and angiogenesis is a critical step in this process. sFLT01 is a novel fusion protein and a dual targeting agent that neutralizes both VEGF and PlGF proangiogenic activities. GRP78 dual effect in tumor growth and angiogenesis could be activated under VEGF stimulation. The current study was designed to investigate the inhibitory impact of sFLT01 protein on VEGF/GRP78 axis. To this point, sFLT01 construct was synthesized, recombinant plasmid was expressed in eukaryotic host cells, sFLT01-HisTag protein was extracted and analyzed. The functional activity of sFLT01 on VEGF-enhanced tube formation and angiogenesis of HUVEC cells were examined. Eventually, the inhibitory impact of sFLT01 on growth and invasiveness and migration of human prostate cancer cell line, DU145, was assessed. Real-time PCR evaluated the level of GRP78 and its effect on the downstream factors; matrix metallopeptidase proteins 2&9 (MMP2&9) along with tissue inhibitor of metalloproteinase proteins1&2 (TIMP1&2). Results According to the data, sFLT01 protein showed modulatory impact on proliferation, invasion, and migration of DU145 cells along with the potential of HUVECs angiogenesis. Real-Time PCR analysis depicted a significant downregulation in GRP78, MMP2 and MMP9 transcripts’ levels, and a subsequent elevation of TIMP1 and TIMP2 expression under sFLT01 stimulation was detected. Conclusion Overall, these data indicated that the inhibitory impact of sFLT01 on cancer cells growth and invasiveness could be mediated through the modulation of VEGF/GRP78/MMP2&9 axis and activation of TIMPs.