bluuebunny/crossref_metadata_2025_split huggingface dataset:
Ozone oil promotes wound healing via increasing miR‐21‐5p‐mediated inhibition of RASA1
Authors: Xiao Wei‐Rong , Wu Meng , Bi Xiang‐Rong 
Published: March 2021

AbstractSkin wound is a very common type of injury and the healing process greatly affects the life quality of individuals. Ozone has been shown beneficial to wound healing with unclear mechanisms. Here, we tested the effect of ozone oil (OZ) on wound healing and investigated the underlying mechanisms. Mouse skin wound model and Masson staining were used to evaluate the effect of OZ on wound healing. Primary fibroblast culture was employed to assess the functions of OZ, miR‐21‐5p, and RASA1. QRT‐PCR and western blot were used to determine expression levels of miR‐21‐5p, RASA1, α‐SMA, and collagen I. CCK‐8 assay and scratch wound healing assay were used to measure viability and migration of fibroblasts. Dual luciferase activity assay was performed to validate miR‐21‐5p/RASA1 interaction. OZ accelerated wound healing in mice and promoted proliferation and migration abilities of fibroblasts. miR‐21‐5p was increased while RASA1 was reduced during the wound healing and OZ treatment augmented those changes, as well as increased levels of α‐SMA and collagen I. Knockdown of miR‐21‐5p suppressed those effects of OZ on fibroblasts. Furthermore, miR‐21‐5p directly targeted RASA1 mRNA and negatively regulated its expression. Overexpression of RASA1 inhibited fibroblast proliferation and migration as well as diminished α‐SMA and collagen I protein expression. Additionally, RASA1 overexpression blocked the promotion of miR‐21‐5p overexpression on fibroblast viability and migration. In vivo, miR‐21‐5p facilitated wound healing while overexpression of RASA1 reversed the effect. OZ promoted wound healing by enhancing miR‐21‐5p‐mediated RASA1 inhibition to increase fibroblast proliferation and migration.
Solution of Two-Dimensional Stokes Flow With Elliptical Coordinates and Its Application to Permeability of Porous Media
Authors: Hellou Mustapha , Lominé Franck , Bourbatache Mohamed Khaled , Hajjam Mohamed 
Published: March 2021

AbstractIn this paper, analytical developments of the biharmonic equation representing two-dimensional Stokes flow are performed with elliptical coordinates. It is found that the streamfunction is expressed with series expansions based on Gegenbauer polynomials of first and second kinds with order one Cn1 and Dn1 for n natural integer number. Application to an elliptical fiber enclosed in an elliptical boundary with uniform flow is made. It is found that a particular solution responsible of the drag must be added to the general solution. Following this, the flow through rectangular arrays of elliptical fibers is studied, and the permeability of this medium is determined as a function of porosity.
A New Approach Utilizing Liquid Catalyst for Improving Heavy Oil Recovery
Authors: Alarbah Ali , Shirif Ezeddin , Jia Na , Bumraiwha Hamdi 
Published: March 2021

AbstractChemical-assisted enhanced oil recovery (EOR) has recently received a great deal of attention as a means of improving the efficiency of oil recovery processes. Producing heavy oil is technically difficult due to its high viscosity and high asphaltene content; therefore, novel recovery techniques are frequently tested and developed. This study contributes to general progress in this area by synthesizing an acidic Ni-Mo-based liquid catalyst (LC) and employing it to improve heavy oil recovery from sand-pack columns for the first time. To understand the mechanisms responsible for improved recovery, the effect of the LC on oil viscosity, density, interfacial tension (IFT), and saturates, aromatics, resin, and asphaltenes (SARA) were assessed. The results show that heavy oil treated with an acidic Ni-Mo-based LC has reduced viscosity and density and that the IFT of oil–water decreased by 7.69 mN/m, from 24.80 mN/m to 17.11 mN/m. These results are specific to the LC employed. The results also indicate that the presence of the LC partially upgrades the structure and group composition of the heavy oil, and sand-pack flooding results show that the LC increased the heavy oil recovery factor by 60.50% of the original oil in place (OOIP). Together, these findings demonstrate that acidic Ni-Mo-based LCs are an effective form of chemical-enhanced EOR and should be considered for wider testing and/or commercial use.
Implementation of a Neural Network into a User-Material Subroutine for Finite Element Simulation of Material Viscoplasticity
Authors: Benabou Lahouari 
Published: March 2021

Abstract
In this study, a neural network is trained to predict the response of a viscoplastic solder alloy based on a reduced data set. The model is shown to accurately describe the behavior of the material for the temperature range from 298 °K to 398 °K and the strain rate range from 2 × 10−5 s−1 to 2 × 10−2 s−1. The model is then implemented in the form of a user subroutine in the finite element code Abaqus to be used for simulations of the material behavior. The implementation requires that the weights and biases of the network are extracted and that its gradients (derivatives of the output with respect to the inputs) are calculated to be passed on to the user subroutine. Finite element (FE) simulations based on the implemented neural network are compared with those based on the physical viscoplastic model of Anand, showing an overall good agreement between both approaches. However, some limitations concerning the neural network ability to predict the transient effects during a strain rate jump or a temperature change are identified and discussed.
Observation of Grease Film Behavior in Sliding-Rolling Concentrated Contacts
Authors: Jin Xuyang , Li Xinming , Chen Qi , Yang Ping , Guo Feng , Jiang Nan 
Published: March 2021

Abstract
In sliding-rolling concentrated contacts, the grease film is subjected to shear action. In this study, grease film behaviors have been observed under different sliding-rolling ratios (SRRs) by measuring film thickness and friction coefficient simultaneously. The evolution patterns of the grease films with disc revolutions are affected by SRR, entrainment speed, and grease consistency. The severe starvation due to film decay at low shear and the film recovery at high shear has been recognized. The grease with low consistency is in a fully flooded state due to improvement in lubricant replenishment. In addition, the corresponding friction coefficient tends to increase or be constant in terms of contact states. The mechanisms of the grease behavior related to shear actions and grease properties are analyzed.
“Just Going to a Spin Class”: Participant Experiences of Inclusion Within an Integrated Indoor Cycling Program
Authors: Auger Joanna M. , Spencer Nancy L.I. 
Published: March 2021

Justifications for access to physical activity for people who experience disability tend to focus on the health benefits associated with a medical model of disability. The result is often programs that are segregated and impairment-focused, with limited access to integrated settings that are also potentially inclusive. In this instrumental case study, the authors engaged 20 participants with and without impairment from an adult integrated indoor cycling program to explore what contributed to meaningful and inclusive experiences in this setting. Data were generated through semistructured interviews and reflective notes. Thematic analysis led to three themes: (a) “just going to a spin class” (b) “seamless”? and (c) “deliberate community.” Using a relational ethics framework, the findings are discussed with regard to their potential to inform the development of integrated and inclusive physical activity programs, with emphasis on program structure and instructor reflexivity and training.
Factors Associated With Penalty Outcome on Male Elite Goalball
Authors: Furtado Otávio Luis Piva da Cunha , Häyrinen Mikko , Alves Isabela dos Santos , Travitzki Leonardo , Morato Márcio Pereira 
Published: March 2021

The authors’ purpose was to examine the factors associated with penalty outcomes of male elite goalball. A total of 122 video-recorded matches from two Paralympic Games (i.e., 2012 and 2016) and the 2014 Goalball World Championship were assessed using notational analysis. Individual (n = 2), situational (n = 4), and performance variables (n = 7) were analyzed with good strength of agreement for intra- and interrater kappa index values. Their results showed that penalties play a very important role in the final score, composing around 25% of total goals in elite goalball matches. Winners were awarded on average with 62% of penalties and had 66% effectiveness compared with losers (31% and 53%) or drawers (8% and 52%). Based on the authors’ findings, penalty takers should direct their throws at specific target sectors on the court, which could increase their rate of success to over 80%.
Associations of Mothers’ and Children’s Stress With Children’s Device-Measured Physical Activity and Sedentary Behavior Trajectories Across 3 Years
Authors: Dunton Genevieve F. , Chu Daniel , Naya Christine H. , Belcher Britni R. , Mason Tyler B. 
Published: March 2021

Background: Psychological stress has adverse effects on health-related behaviors, yet longitudinal research is lacking. Research examined how children’s and mothers’ perceived stress are associated with children’s physical activity and sedentary behavior trajectories across 3 years. Methods: Mothers and their children (N = 186 dyads; 8–12 y at baseline, 57% Hispanic) completed 6 assessments across 3 years. Children and mothers self-reported perceived stress using the Stress in Children Scale and Perceived Stress Scale, respectively. Children’s moderate to vigorous physical activity (MVPA) and sedentary behavior were assessed using accelerometers. Mixed models examined interactions of mothers’ and children’s perceived stress by time elapsed in the study on children’s MVPA and sedentary behavior. Results: The perceived stress × time elapsed interactions were significant for children’s MVPA and sedentary behavior (Ps < .05). Higher average perceived stress in mothers was associated with greater decreases in children’s MVPA and increases in children’s sedentary behavior. The child stress × time elapsed interactions was significant for children’s MVPA (P < .05) but not sedentary behavior. Higher average perceived stress in children was associated with smaller decreases in children’s MVPA. Conclusion: Interventions to promote physical activity and reduce screen time in children should mitigate the effects of psychological stress, especially among mothers, on these behaviors.
Intrasession and Intersession Reliability of Isometric Trunk and Hip Strength Measurements Using the Portable Tension Dynamometer
Authors: Oyama Sakiko , Garza Edgar , Dugan Kylie 
Published: March 2021

Context: The trunk/pelvis is an important link between the upper- and lower-extremities. Therefore, assessing strength of the trunk and hip muscles that control the segments is clinically meaningful. While an isokinetic dynamometer can be used to measure trunk strength, the equipment is expensive and not portable. Objective: To test the reliability of simple trunk and hip strength measures that utilize a bar, straps, and a portable tension dynamometer. Design: Test–retest reliability study. Setting: Biomechanics research laboratory. Patients (or Other Participants): Twenty college-age individuals (10 males/10 females, age = 20.9 [3.7] y) participated. Intervention(s): The participants attended 2 testing sessions, 1 week apart. The participants’ trunk-flexion, rotation, and hip abduction strength were measured at each session. Main Outcome Measures: Peak trunk flexion, rotation, and hip abduction forces were normalized to the participant’s body weight (BW). In addition, hip-abduction torque was calculated by multiplying the force times the leg length and normalized to BW. The trial data from both sessions were used to calculate the intrasession reliability, and the averages from the 2 sessions were used to calculate the intersession reliability. Intraclass correlation coefficients, SEM, and minimal detectable change were calculated to evaluate reliability of measures. Results: The intrasession intraclass correlation coefficients (SEM) for trunk flexion, rotation, hip abduction, and hip abduction torque were .837 (5.2% BW), .978 (1.3% BW), .955 (1.0% BW), and .969 (5.8 N·m/BW), respectively. The intersession reliability for trunk flexion, rotation, hip abduction, and hip abduction torque were .871 (4.3% BW), .801 (3.8% BW), .894 (1.5% BW), and .968 (5.9 N·m/BW), respectively. Conclusions: The measures of trunk and hip abduction strength are highly repeatable within a session. The reliability of the measures between sessions was also good/excellent with relatively small SEM and minimal detectable change. The tests described in this study can be used to assess changes in trunk/hip strength over time.
Melatonin for non-operating room sedation in paediatric population: a systematic review and meta-analysis
Authors: Ahmed Javed , Patel Waseemoddin , Pullattayil Abdul Kareem , Razak Abdul 
Published: March 2021

ContextThe literature on melatonin as a sedative agent in children is limited.ObjectiveTo conduct a systematic review of studies assessing the efficacy and safety of melatonin for non-operating room sedation in children.MethodsMedline, Embase, Cochrane Library and Cumulative Index to Nursing and Allied Health were searched until 9 April 2020 for studies using melatonin and reporting one of the prespecified outcomes of this review. Two authors independently assessed the eligibility, risk of bias and extracted the data. Studies with a similar study design, comparator and procedure were pooled using the fixed-effect model.Results25 studies (clinical trials=3, observational studies=9, descriptive studies=13) were included. Melatonin was used for electroencephalogram (EEG) (n=12), brainstem evoked response audiometry (n=8) and magnetic resonance imaging (MRI) (n=5). No significant differences were noted on meta-analysis of EEG studies comparing melatonin with sleep deprivation (SD) (relative risk (RR) 1.06 (95% CI 0.99 to 1.12)), melatonin with chloral hydrate (RR 0.97 (95% CI 0.89 to 1.05)) and melatonin alone with melatonin and SD combined (RR 1.03 (95% CI 0.97 to 1.10)) for successful procedure completion. However, significantly higher sedation failure was noted in melatonin alone compared with melatonin and SD combined (RR 1.55 (95% CI 1.02 to 2.33)) for EEG. Additionally, meta-analysis showed lower sleep latency for melatonin compared with SD (mean difference −10.21 (95% CI −11.53 to −8.89) for EEG. No major adverse events were reported with melatonin.ConclusionAlthough several studies were identified, and no serious safety concerns were noted, the evidence was not of high quality to establish melatonin’s efficacy for non-operating room sedation in children.
Development and evaluation of a deep learning model for the detection of multiple fundus diseases based on colour fundus photography
Authors: Li Bing , Chen Huan , Zhang Bilei , Yuan Mingzhen , Jin Xuemin , Lei Bo , Xu Jie , Gu Wei , Wong David Chuen Soong , He Xixi , Wang Hao , Ding Dayong , Li Xirong , Chen Youxin , Yu Weihong 
Published: March 2021

AimTo explore and evaluate an appropriate deep learning system (DLS) for the detection of 12 major fundus diseases using colour fundus photography.MethodsDiagnostic performance of a DLS was tested on the detection of normal fundus and 12 major fundus diseases including referable diabetic retinopathy, pathologic myopic retinal degeneration, retinal vein occlusion, retinitis pigmentosa, retinal detachment, wet and dry age-related macular degeneration, epiretinal membrane, macula hole, possible glaucomatous optic neuropathy, papilledema and optic nerve atrophy. The DLS was developed with 56 738 images and tested with 8176 images from one internal test set and two external test sets. The comparison with human doctors was also conducted.ResultsThe area under the receiver operating characteristic curves of the DLS on the internal test set and the two external test sets were 0.950 (95% CI 0.942 to 0.957) to 0.996 (95% CI 0.994 to 0.998), 0.931 (95% CI 0.923 to 0.939) to 1.000 (95% CI 0.999 to 1.000) and 0.934 (95% CI 0.929 to 0.938) to 1.000 (95% CI 0.999 to 1.000), with sensitivities of 80.4% (95% CI 79.1% to 81.6%) to 97.3% (95% CI 96.7% to 97.8%), 64.6% (95% CI 63.0% to 66.1%) to 100% (95% CI 100% to 100%) and 68.0% (95% CI 67.1% to 68.9%) to 100% (95% CI 100% to 100%), respectively, and specificities of 89.7% (95% CI 88.8% to 90.7%) to 98.1% (95%CI 97.7% to 98.6%), 78.7% (95% CI 77.4% to 80.0%) to 99.6% (95% CI 99.4% to 99.8%) and 88.1% (95% CI 87.4% to 88.7%) to 98.7% (95% CI 98.5% to 99.0%), respectively. When compared with human doctors, the DLS obtained a higher diagnostic sensitivity but lower specificity.ConclusionThe proposed DLS is effective in diagnosing normal fundus and 12 major fundus diseases, and thus has much potential for fundus diseases screening in the real world.
North Carolina macular dystrophy shows a particular drusen phenotype and atrophy progression
Authors: Birtel Johannes , Gliem Martin , Herrmann Philipp , Neuhaus Christine , Holz Frank G , MacLaren Robert E , Scholl Hendrik P N , Charbel Issa Peter 
Published: March 2021

Background/AimTo provide a comprehensive multimodal retinal imaging characterisation of patients with North Carolina macular dystrophy (NCMD).MethodsClinical evaluation and retinal imaging in six families.ResultsTwenty-one subjects showed phenotypic characteristics of NCMD . Small drusen-like deposits were found in all affected individuals, either tightly grouped in the macula, or surrounding atrophic or fibrotic macular alterations. These small subretinal lesions showed an increased fundus autofluorescence and were associated with only mild irregularities on optical coherence tomography imaging. Similar drusen-like deposits were regularly seen in the peripheral fundus, predominantly temporally and often with a radial distribution. Two patients showed a bilateral chorioretinal atrophy and two had a macular neovascularisation (MNV). Findings from follow-up examinations were available from 11 patients. The retinal phenotype remained overall stable, except for two patients: one patient with atrophy showed a distinct growth of the atrophic lesions on longitudinal AF imaging over a review period of 14 years. One patient with MNV showed a unilateral decline of best-corrected visual acuity. Genetic testing identified the single nucleotide variant chr6:100040987G>C upstream of thePRDM13gene in all family members with NCMD phenotype.ConclusionPatients with NCMD show a characteristic retinal phenotype and distribution of drusen that differ from drusen in patients with age-related macular degeneration. Although the prognosis of this developmental condition is overall better than for other macular diseases with drusen, patients may be at risk of developing MNV or enlargement of pre-existing atrophy.
Role of anterior segment optical coherence tomography angiography in assessing limbal vasculature in acute chemical injury of the eye
Authors: Ang Marcus , Foo Valencia , Ke Mengyuan , Tan Bingyao , Tong Louis , Schmetterer Leopold , Mehta Jodbhir S 
Published: March 2021

PurposeTo study the role of two anterior segment optical coherence tomography angiography (AS-OCTA) systems in eyes with acute chemical injury.MethodsProspective study in subjects with unilateral chemical injuries. Sequential slit-lamp assessment with spectral domain (SD) (AngioVue, Optovue, USA) and swept source (SS) (Plex Elite, Zeiss, Carl Zeiss Meditec, Dublin, California, USA) AS-OCTA was performed in both eyes within 24–48 hours of injury. Subjects were managed with a standard clinical protocol and followed-up for 3 months. We assessed limbal disruption (loss of normal limbal vessel architecture), limbal vessel density measurements and agreement (kappa coefficient, κ) between masked assessors of limbal disruption based on AS-OCTA scans and slit-lamp assessment.ResultsTen subjects with median age 31 (25–33) years, 20% women, 60% suffered alkali injuries (Roper-Hall grade 1.5±0.7, Dua grade 2.3±1.2) at presentation. Mean limbal vessel density was lower in quadrants of affected eyes compared with controls detected by SD AS-OCTA (9.4%±2.0% vs 15.5%±1.8%, p<0.001) and SS AS-OCTA (8.8%±2.5% vs 13.9%±1.3%, p=0.01). There was substantial agreement when assessing limbal disruption on AS-OCTA (κ=0.7) compared with slit-lamp evaluation (κ=0.4). Overall, we found good agreement between SD and SS AS-OCTA systems in assessing limbal vessel density in eyes with chemical injury at presentation (mean paired difference: −1.08, 95% CI −3.2 to 0.5; p=0.189).ConclusionsIn this pilot study, AS-OCTA provided objective, non-contact, rapid assessment of limbal vasculature involvement in eyes with acute chemical injury. Further studies are required to establish the role of AS-OCTA in determining the prognosis of eyes with chemical injury.
Loss of ARID1A activates mTOR signaling and SOX9 in gastric adenocarcinoma—rationale for targeting ARID1A deficiency
Authors: Dong Xiaochuan , Song Shumei , Li Yuan , Fan Yibo , Wang Lulu , Wang Ruiping , Huo Longfei , Scott Ailing , Xu Yan , Pizzi Melissa Pool , Ma Lang , Wang Ying , Jin Jiangkang , Zhao Wei , Yao Xiaodan , Johnson Randy L , Wang Linghua , Wang Zhenning , Peng Guang , Ajani Jaffer A 
Published: March 2021

BackgroundGastric adenocarcinoma (GAC) is a lethal disease with limited therapeutic options. Genetic alterations in chromatin remodelling gene AT-rich interactive domain 1A (ARID1A) and mTOR pathway activation occur frequently in GAC. Targeting the mechanistic target of rapamycin (mTOR) pathway in unselected patients has failed to show survival benefit. A deeper understanding of GAC might identify a subset that can benefit from mTOR inhibition.MethodsGenomic alterations in ARID1A were analysed in GAC. Mouse gastric epithelial cells from CK19-Cre-Arid1Afl/fl and wild-type mice were used to determine the activation of oncogenic genes due to loss of Arid1A. Functional studies were performed to determine the significance of loss of ARID1A and the sensitivity of ARID1A-deficient cancer cells to mTOR inhibition in GAC.ResultsMore than 30% of GAC cases had alterations (mutations or deletions) of ARID1A and ARID1A expression was negatively associated with phosphorylation of S6 and SOX9 in GAC tissues and patient-derived xenografts (PDXs). Activation of mTOR signalling (increased pS6) and SOX9 nuclear expression were strongly increased in Arid1A−/− mouse gastric tissues which could be curtailed by RAD001, an mTOR inhibitor. Knockdown of ARID1A in GAC cell lines increased pS6 and nuclear SOX9 and increased sensitivity to an mTOR inhibitor which was further amplified by its combination with fluorouracil both in vitro and in vivo in PDXs.ConclusionsThe loss of ARID1A activates pS6 and SOX9 in GAC, which can be effectively targeted by an mTOR inhibitor. Therefore, our studies suggest a new therapeutic strategy of clinically targeting the mTOR pathway in patients with GAC with ARID1A deficiency.