bluuebunny/crossref_metadata_2025_split huggingface dataset:
Appetite Regulation in Response to Acute Exercise among Weight-Loss Treatment Responders vs Nonresponders: A Pilot Study
Authors: DiPietro Loretta , Malin Steven K. , Barbiero Matthew , Levers Kyle , Heinz Eric , Napolitano Melissa A. 
Published: January 2025

ABSTRACT

Introduction
The reason for weight-loss heterogeneity in response to lifestyle interventions is not clear. Important regulators of food intake and energy expenditure include peripheral hormonal signals from insulin, glucagon-like peptide 1 (GLP-1), leptin, and ghrelin. Herein, we examine the hormonal regulators of appetite and satiety following a high-volume bout of moderate-to-vigorous intensity exercise in responders and nonresponders to a 6-month, digitally delivered, weight-loss lifestyle intervention.

Methods
Young adults (27.7 ± 3.7 yr; 11 female, 1 male) characterized as responders (n = 7; lost 3% of their initial body weight) and nonresponders (n = 5) participated in a 60-min treadmill exercise challenge, consisting of three 15-min intervals at 80% of peak oxygen consumption (V̇O2peak), with 5 min of walking at 50% V̇O2peak between intervals. Blood samples were obtained prior to and immediately after exercise and 30 and 60 min after exercise during seated recovery for determination of plasma glucose, insulin, GLP-1active, leptin, and acylated ghrelin. Univariate analyses (mean ± standard deviation) and Cohen’s d described between-group differences in hormone responses to exercise.

Results
Glucose and leptin responses during the exercise challenge and recovery were similar for nonresponders and responders; however, nonresponders had higher insulin concentrations post-exercise compared to responders (9.8 ± 3.5 μU·mL−1 vs 6.2 ± 2.2 μU·mL−1 at 30 min (d = 1.23); 9.4 ± 1.8 μU·mL−1 vs 6.3 ± 2.1 μU·mL−1 at 60 min (d = 1.54)). Concentrations of GLP-1active also appeared higher in nonresponders compared to responders following exercise (12.2 ± 2.8 pmol·L−1 vs 9.3 ± 1.1 pmol·L−1 (d = 1.36)), but this difference disappeared after 30 min of recovery. Ghrelin levels were lower in the nonresponders following exercise (308 ± 27 pg·mL−1 vs 494 ± 98 pg·mL−1 (d = −2.58)) and remained so over the recovery period (287.4 ± 12.3 pg·mL−1 vs 392.1 ± 47.2 pg·mL−1 at 30 min (d = −3.08); 313.0 ± 39.8 pg·mL−1 vs 393.9 ± 60.8 pg·mL−1 at 60 min (d = −1.21)).

Conclusions
These findings support potential appetite hormonal differences in the heterogenous weight-loss response to lifestyle therapy. A better understanding of how exercise affects appetite regulation in young adults with overweight and obesity could help with precision care.
Magnitude of telemedicine utilization and associated factors among health professionals working at selected public hospitals in Southern Ethiopia
Authors: Fikrie Anteneh , Daniel Dawit , Ermiyas Samrawit , Hassen Hawa , Seyoum Wongelawit , Kebede Seyoum , Wako Wako Golicha 
Published: January 2025

Background
Despite the immense potential of telemedicine, its implementation in Ethiopia and other developing nations has faced formidable challenges, leading to disappointingly low utilization rates. Therefore, this study sought to assess the magnitude and factors associated with telemedicine service practice among healthcare professionals in the pilot public hospitals of Sidama and Southern Nations Nationalities Peoples Regions.

Methods
Cross-sectional study was conducted from June 1–30, 2021 among randomly selected 407 health professionals working at Pilot Hospitals in Southern Ethiopia. A pretested and structured self-administered questionnaire was used to collect the socio-demographic, knowledge and attitude of Health Professionals towards telemedicine and health system-related data. Data were coded and entered using Epi-data version 4.6. and exported to SPSS version 20 for analysis. Bi-variable and multivariable binary logistic regression was done to identify factors associated with telemedicine utilization. A P-value<0.05 and Adjusted odds ratio (AOR) together with 95% Confidence Interval (CI) was used to declare statistical significance. The data were presented by tables, text and figures and charts.

Results
The study found that 34.6% (95% CI: 30–39.6%), 54.1% (95% CI: 49.6–59.2%), and 26% (95% CI: 21.6–30.2%) of the respondents have good knowledge, a positive attitude, and practiced telemedicine service, respectively. Age ≥ 36 years (AOR = 2.99, 95% CI: 1.18–7.60), being a medical doctor (AOR = 3.91, 95% CI 1.15–13.25), having good knowledge (AOR = 2.75, 95% CI 1.54–4.89), presence of an information sharing culture (AOR = 3.95, 95% CI 1.16–13.45), presence of a practicing platform (AOR = 3.01, 95% CI 1.06–8.53), and presence of government commitment (AOR = 2.52, 95% CI 1.09–5.82) were found to be significantly associated with telemedicine service utilization.

Conclusion
Despite positive attitudes, the adoption of telemedicine among healthcare professionals in the study area remains limited. Factors such as age, profession, knowledge, and cultural factors influence its uptake. To promote wider adoption and address challenges, governments should: implement comprehensive guidelines, training programs, and platforms for healthcare professionals to effectively utilize telemedicine technologies can accelerate healthcare delivery in the study area.
Effectiveness of robot-assisted task-oriented training intervention for upper limb and daily living skills in stroke patients: A meta-analysis
Authors: Jin Chengzhu , Chen Yonghuan , Ma Yuanyuan 
Published: January 2025

Purpose
Stroke is one of the leading causes of acquired disability in adults in high-income countries. This study aims to determine the intervention effects of robot-assisted task-oriented training on enhancing the upper limb function and daily living skills of stroke patients.

Methods
A systematic search was conducted across PubMed, China National Knowledge Infrastructure, Web of Science, Cochrane Library, Embase, and Scopus databases through March 1, 2024. This process yielded 1,649 articles, from which 15 studies with 574 samples met the inclusion criteria for analysis. The quality of the included studies was evaluated using the Cochrane Risk of Bias tool. We performed meta-analyses, subgroup analyses, regression analyses, and sensitivity analyses using Review Manager 5.4 and Stata 17.0. Furthermore, publication bias was assessed using Begg’s and Egger’s tests. This study is registered with PROSPERO (No. CRD42024513483).

Results
A random effects model was utilized. The results indicated that robot-assisted task-oriented training significantly improved Fugl-Meyer Assessment-Upper Extremity scores compared to the control group [SMD = 1.01, 95% CI (0.57, 1.45)]. Similarly, robot-assisted task-oriented training demonstrated a significant effect on the Modified Barthel Index scores [SMD = 0.61, 95% CI (0.41, 0.82)]. Subgroup and regression analyses revealed that the use of combined interventions, the geographical region of the first author, and the age of the subjects did not appear to be sources of high heterogeneity. Publication bias tests using the FMA-UE as an outcome measure yielded Begg’s test (p = 0.76) and Egger’s test (p = 0.93), suggesting no significant publication bias. Sensitivity analyses confirmed the robustness of the study findings.

Conclusions
Robot-assisted task-oriented training significantly enhances the rehabilitation of upper limb function and the recovery of daily living skills in stroke patients.
Kniest dysplasia in girl aged 4 years: a case report
Authors: Sahoo Ipsita , Jena Nitish , Dash Mrutunjay , Bisoi Swarup 
Published: January 2025

Kniest dysplasia is a rare chondrodysplasia characterized by distinct musculoskeletal and craniofacial abnormalities. Additional features like hearing loss and some ocular abnormalities can also be present. We report a case of Kniest dysplasia from eastern India with a rare mutation confirmed by genetic testing. A 4-year-old girl presented with skeletal deformities with normal intelligence. On examination, she had dysmorphism, short stature, barrel-shaped chest, scoliosis, short limbs with enlarged joints, and dental problems. The skeletal survey showed platyspondyly, epiphyseal enlargement, and a hypoplastic femoral head with cloud-like calcification. Clinical suspicion of Kniest dysplasia was kept based on characteristic skeletal abnormalities and radiological findings. The diagnosis was confirmed by genetic analysis, which revealed a mutation in the COL2A1 gene (c.905C>T) on chromosome 12 exon 14.
Improvement of Sexed Sperm Quality of Bali Bulls by Adding Palmyra (Borassus flabellifer Linn.) Fruit Water to Citrate-Egg Yolk Extender
Authors: Hine Thomas Mata , Nalley Wilmientje Marlene , Marawali Aloysius , Kihe Johny Nada , Kune Petrus , Uly Kirenius 
Published: January 2025

Sperm sexing is a technique designed to separate sperm carrying the X and Y chromosomes, which are then used for artificial insemination to generate offspring with the sex the breeder desires. However, the sexing process causes a decrease in sperm quality caused by oxidative stress due to an excessive increase in free radicals. Thus, to counteract the detrimental effects of free radicals on sperm life, antioxidants must be added to the diluent. This study was designed by adding palmyra fruit water (PFw) to citrate-egg yolk (CEy) diluent. This study aimed to evaluate the potency of PFw as a natural antioxidant supplement in CEy diluent to improve the quality of sexing sperm in Bali bulls. Sperm were collected using the artificial vagina method from three Bali bulls aged 3-4 years. Good quality sperm (motility ?70%, abnormality ?20%) were sexed with a three-layer albumin gradient method (5, 10, and 15 percent) for 20 minutes, and sperm that were on the bottom albumin layer were preserved in CEy, PFw, PFw-egg yolk (PFw-Ey), or CEy-PFw. The results showed that sperm preservation in the CEy-PFw diluent resulted in higher sperm quality (P0.05) compared to the other three diluents, except for the sperm abnormality parameter (P0.05). It was concluded that adding PFw into the CEy diluent could potentially improve the sexed sperm quality of Bali bulls.
Diversity of the Monoamine Oxidase A Gene in Beef Cattle
Authors: Siregar Nadira Andarini , Noor Ronny Rachman , Priyanto R , Ulum Mokhamad Fakhrul , Jakaria Jakaria 
Published: January 2025

Monoamine Oxidase A (MAOA) is a gene that controls aggressive traits. The MAOA gene plays a role in encoding the monoamine oxidase A enzyme, which plays a role in the catabolism of neurotransmitters, including dopamine, norepinephrine, and serotonin. This study aims to identify the diversity of the MAOA SNP gene in beef cattle using sequencing methods. This research used 127 cattle DNA samples to identify diversity, including Bali, Limousin, Wagyu, PO, Madura, and Wagyu-Bali (F1) cattle. MAOA gene polymorphisms in the promoter and exon 1 were analyzed using sequencing methods. Genotype frequencies, allele frequencies, heterozygosity values, and Hardy-Weinberg balance were calculated using the PopGen32 program. The results showed that the MAOA gene in the promoter region has six SNPs, one of which is SNP g.385GA, while the MAOA gene in exon 1 is monomorphic. The PCR-RFLP method was used to investigate the SNP g.385GA MAOA gene polymorphism using the RSaI restriction enzyme. The MAOA gene was detected in 3 genotypes: GG, GA, and AA. SNP g.385GA is polymorphic in Bali, PO, Madura, and Wagyu-Bali cross (F1) cattle while monomorphic in Limousin and Wagyu cattle. Further studies are necessary to explore the functional implications of SNP g.385GA and their relationship to aggressive behaviors in cattle.
Animal Welfare Study in Sheep Transported with Methionine Hydroxy Analog and Dextrose Supplementation
Authors: Baihaqi Muhamad , Nuraini Henny , Jayanegara Anuraga , Manalu Wasmen 
Published: January 2025

Livestock transportation adversely affects animal welfare and performance during subsequent production periods. This research aimed to evaluate the effects of methionine hydroxy analog (MHA) and dextrose supplementations before transportation on the animal welfare status of sheep. The study used 27 thin-tailed male sheep with an average body weight of 21,8±2,0 kg, 10-12 months of age, which were divided randomly into three treatments: sheep without transportation and additional supplement (K: Control); sheep transported without additional supplement (P1); sheep transported with additional supplement (P2: MHA 0.5 g/kg body weight and dextrose 0.5 g/kg body weight). Sheep in groups P1 and P2 were transported for 8 hours using commercial vehicles usually used by local farmers. The results showed that sheep in the P2 group had significantly lower body weight loss than those in the P1 group and faster recovery time (P0.05). Sheep in the P2 group also demonstrated better post-transportation behavior than those in P1, with no significant difference from the control group. The experimental sheep in the P2 group had a significantly lower N/L ratio than those in P1 and was comparable to the control group (P0.05). The P2 group also has significantly lower cortisol hormone and blood glucose concentrations than P1, which indicates improved sheep welfare status in P2 and Control groups. Therefore, it can be concluded that supplementation of MHA and dextrose before departure improves sheep's welfare status under transportation.
Germline deletion of Rgs2 and/or Rgs5 in male mice does not exacerbate left ventricular remodeling induced by subchronic isoproterenol infusion
Authors: Dahlen Shelby , Mohanty Ipsita , Sun Bo , Nallapaneni Sanjana , Osei‐Owusu Patrick 
Published: January 2025

AbstractSympathoexcitation is a hallmark of heart failure, with sustained β‐adrenergic receptor (βAR)‐G protein signaling activation. βAR signaling is modulated by regulator of G protein signaling (RGS) proteins. Previously, we reported that Gαi/o regulation by RGS2 or RGS5 is key to ventricular rhythm regulation, while the dual loss of both RGS proteins results in left ventricular (LV) dilatation and dysfunction. Here, we tested whether sustained βAR stimulation with isoproterenol (ISO, 30 mg/kg/day, 3 days) exacerbates LV remodeling in male mice with germline deletion of Rgs2 and/or Rgs5. Rgs2 KO and Rgs2/5 dbKO mice showed LV dilatation at baseline, which was unchanged by ISO. Rgs2 or Rgs5 deletion decreased Rgs1 expression, whereas Rgs5 deletion increased Rgs4 expression. ISO induced cardiac hypertrophy and interstitial fibrosis in Rgs2/5 dbKO mice without increasing cardiomyocyte size or LV dilation but increased expression of cardiac fetal gene Nppa, α‐actinin, and Ca2+‐/calmodulin‐dependent kinase II. Single Rgs2 and Rgs5 KO mice had markedly increased CD45+ cells, whereas tissue from Rgs5 KO mice showed increased CD68+ cells, as revealed by immunohistochemistry. The results together indicate that ventricular remodeling due to Rgs2 and/or Rgs5 deletion is associated with augmented myocardial immune cell presence but is not exacerbated by sustained βAR stimulation.
Behind the scenes of EQA – characteristics, capabilities, benefits and assets of external quality assessment (EQA)
Authors: Buchta Christoph , Marrington Rachel , De la Salle Barbara , Albarède Stéphanie , Badrick Tony , Berghäll Heidi , Bullock David , Coucke Wim , Delatour Vincent , Geilenkeuser Wolf-Jochen , Griesmacher Andrea , Henriksen Gitte M. , Huggett Jim F. , Luppa Peter B. , Pelanti Jonna , Pezzati Paola , Sandberg Sverre , Spannagl Michael , Thelen Marc , Restelli Veronica , Perrone Lucy A. 
Published: January 2025

Abstract
External quality assessment (EQA) cycles are the smallest complete units within EQA programs that laboratories can use to obtain external assessments of their performance. In each cycle, several samples are distributed to the laboratories registered for participation, and ideally, EQA programs not only cover the examination procedures but also the pre- and post-examination procedures. The properties and concentration range of measurands in individual samples are selected with regard to the intended challenge for the participants so that each sample fulfils its purpose. This aims to ensure the most significant possible information gain in every cycle using the lowest possible number of EQA samples and thus, under economically optimal conditions. Participants examine samples and the results are reported to the EQA provider, who compares them with the target values for individual measurands in every sample. The EQA provider assesses the laboratory performance, and finally communicates the assessment results to the participant. The participants evaluate the outcomes of the assessment of their examination results and can draw conclusions in the case of both failing and passing and, if necessary, define improvement measures. After completion, each cycle is evaluated by the provider so that limitations and weaknesses of the EQA program can be identified and appropriate measures taken, or to confirm its continued suitability and appropriateness.
EN ISO 15189 revision: EFLM Committee Accreditation and ISO/CEN standards (C: A/ISO) analysis and general remarks on the changes
Authors: Linko Solveig , Boursier Guilaine , Bernabeu-Andreu Francisco A. , Dzneladze Nana , Vanstapel Florent , Brguljan Pika Meško , Tosheska-Trajkovska Katerina , Mehay Hélène , Panteghini Mauro , Brugnoni Duilio , Milinkovic Neda , Lohmander Maria , Šprongl Luděk , Çubukçu Hikmet Can , Thelen Marc 
Published: January 2025

Abstract
The EN ISO 15189:2022 standard, titled “Medical laboratories – Requirements for quality and competence,” is a significant update to the regulations for medical laboratories. The revised standard was published on December 6, 2022, replacing both EN ISO 15189:2012 and EN ISO 22870:2016. Key objectives of the revision include: 1. Alignment with ISO/IEC 17025:2017, 2. Removal of unintended prescription, 3. Focus on patient interest and safety, 4. Minimization of new requirements, and 5. Improved clarity of text. Dedicating to harmonizing accreditation processes across Europe the EFLM Committee on Accreditation and ISO/CEN standards (C: A/ISO) has produced this guidance document to assist the laboratory medicine community in understanding and implementing the criteria of the EN ISO 15189 revision. Two main objectives of the guidance in educating both laboratories and accreditation bodies with their assessors as well as other stakeholders in laboratory medicine were agreed on. Firstly, to clarify the relevant changes covering all paragraphs of the standard and secondly to make an impact analysis on previous C: A/ISO guidance documents.
Students’ Perceptions of PBL Usefulness
Authors: Babo Lurdes , Mendonça Jorge , Pinto Carla 
Published: January 2025

Abstract
Problem-based learning (PBL) is a teaching technique in which students’ learning is conveyed using real-world problems. However, the implementation of PBL is confronted with several challenges, namely the involvement of students, the definition of the teacher’s role, and the development of effective assessment mechanisms. This paper describes and analyses a teaching framework based on PBL followed in the Differential and Integral Calculus and Statistical Models courses in a Biomedical Engineering Bachelor, at the School of Engineering, Polytechnic of Porto. In order to assess students’ points of view about the effectiveness of this PBL approach with regard to knowledge acquisition, development of soft skills, and assessment methods, a questionnaire was administered at the terminus of the semester. Data analysis revealed that newcomers are more likely to use PBL, while second-year students feel more pressure and report more workload with this type of assessment. In this regard, we consider that factors such as maturity, education level, workload in other courses, and prior PBL experience may influence students’ willingness to adhere to PBL.
Multifunctional characterisation of pressureless sintered Al2O3 –CaTiO3 nanocomposite
Authors: Randhari July , Mallik Prafulla Kumar , Senapati Sukant Kumar 
Published: January 2025

Abstract
This paper highlights the synthesis and processing of alumina−calcium titanate (Al2O3–CaTiO3) composite for multifunctional application in the biomedical industry. In this work, the Al2O3–CaTiO3 composite was synthesized using sol-gel techniques. Calcium oxide, aluminum isopropoxide, titanium isopropoxide, ethanol, and nitric acid were used as precursor chemicals, solvents, stabilizers, and catalysis, respectively. The powder was calcined at a temperature of 800 °C for 1 h. The densification and sintering behavior of uniaxially pressed calcined powder was carried out using a pressureless sintering electric furnace at a temperature of 1,400 °C for 2 h. X-ray diffraction and scanning electron microscopy analysis were used to determine the phases and morphology of powders and sintered nano-composite. The impedance analysis was used to investigate the electrical properties of the sintered composite. As a result, the phases and morphology of Al2O3 and CaTiO3 were confirmed by X-ray diffraction, scanning electron microscopy and high resolution transmission electron microscopy analysis. Impedance analysis indicates the increasing dielectric constant and AC conductivity of the sintered sample with the function of temperature and frequency. Finally, it can be concluded that the biocompatibility of both phases could potentially be fabricated and used in biomedical industries as a functional biomaterials application.
Investigative optimization of nonlinear ion-ion interactions in thulium–erbium doped cascaded fiber amplifier for S + C band
Authors: Sarma Krishna , Khan Mohd Mansoor 
Published: January 2025

Abstract
A combination of a thulium-doped fiber amplifier (TDFA) and an erbium-doped fiber amplifier (EDFA) in a sequential configuration has been identified to design a hybrid amplifier. The proposed amplifier module delivers a flat and high gain. This method is potentially an effective strategy to fully utilize the S + C band (1,460 nm–1,565 nm) for amplifying fiber optic signals. The proposed TDF-EDF hybrid amplifier has an optimum average flat-band gain of 24.27 dB from 1,460 nm to 1,516 nm with a maximum gain of 47.07 dB at 1,528 nm. The parametric optimization has been performed considering the optical pumping configuration with corresponding power, input-signal power, amplifier length, ion density with its radius, and the hybrid amplifier’s numerical aperture (NA). The nonlinear clustering-ion effects known as the ion-ion interaction mechanism (IM) have been aimed to distort the signal gain with broadband flat-gain profile minimally. Apparently, the optimized parameter reduced the gain deterioration by the IM effect to 1.12 % with homogeneous up-conversion (HUC) and 8.14 % with the combined effect of HUC and pair-induced quenching (PIQ) corresponding to the maximum gain. In the flat-band region, the HUC and HUC + PIQ combination have the respective gain deteriorating contributions of 0.67 % and 5.24 %. For the input signal power of −40 dBm, the gain flatness of the hybrid amplifier is obtained as 3.27 dB for 1,460 nm–1,516 nm regions without IM effects. However, with HUC and HUC + PIQ, gain flatness has been reduced to 3.08 dB and 2.19 dB, respectively. Furthermore, the gain variation ratio (GVR) of 0.05 (preferably GVR

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0.4) is obtained from 1,472 nm to 1,504 nm wavelength region indicating a flatter gain response.