bluuebunny/crossref_metadata_2025_split huggingface dataset:
Barriers and Facilitators of Physical Activity Participation in Adolescent Girls: A Systematic Review of Systematic Reviews
Authors: Duffey Keeva , Barbosa Ana , Whiting Stephen , Mendes Romeu , Yordi Aguirre Isabel , Tcymbal Antonina , Abu-Omar Karim , Gelius Peter , Breda João 
Published: October 2021

Background: Persistent low physical activity (PA) levels among adolescent girls constitute a public health concern that calls for immediate and evidence-based policy action. This systematic review (SR) aimed to summarize evidence from SRs examining the barriers and facilitators of PA participation in adolescent girls. The objectives were to provide a synthesis of the available evidence and identify key areas for fostering gender-responsive action and policy implications.Methods: A comprehensive search of relevant SR and meta-analyses were performed in PubMed and Cochrane Library, until February 2021. Studies were included if they were SRs or meta-analyses, included adolescent girls aged between 10 and 19 years, and described barriers or facilitators of PA. Two independent authors performed the screening of potentially eligible studies and both assessed the methodological quality of included studies using the AMSTAR 2 tool. The barriers and facilitators were synthesized at environmental, interpersonal, and individual levels.Results: A total of eight SRs were included in the qualitative synthesis. The most frequent barriers identified were the lack of support from peers, family, and teachers, and the lack of time. The most reported facilitators were weight loss, and support from peers, family, and teachers. Key areas for action and policy implementation include an inclusive approach to curriculum development to address gender norms; adequate training of professionals so they have a range of skills to ensure inclusion of adolescent girls; environmental changes in and out of schools to stimulate participation, to allow adolescent girls to be active in a safe and attractive environment; multistakeholder support at local, regional, and national level in incorporating a gender-responsive approach toward PA participation.Conclusion: The results highlight a variety of factors that influences the PA participation of adolescent girls. For the attainment of effective policies that increase PA levels in adolescent girls, it is essential to engage several stakeholders at different levels in incorporating a gender-responsive approach toward PA participation.Systematic Review Registration: PROSPERO, identifier: CRD42020204023.
Development of a Freshness Index for Fruit Quality Assessment—Using Bell Pepper as a Case Study
Authors: Althaus Bernhard , Blanke Michael 
Published: October 2021

(1) Background: This special issue contains new developments in the field of fresh produce quality. Freshness provides the appeal for the consumer to purchase a particular horticultural produce. Freshness is a combination of size, colour, shape, flesh firmness, turgescence and glossiness without wilting, which imply that the produce has been picked only a short while ago and has not suffered any decay during harvest, transport or storage. (2) Objective: The objective of the present work was to develop potential freshness indices. The indices are based on non-invasive, real-time measurements, using changes in surface appearance after harvest, using Bell pepper as the most difficult model. (3) Methods: The selection criteria were a dramatic change in values over 14 days of storage at 17 °C, and either a consistent decrease or increase without peaks and troughs. Only two (out of five) non-invasive techniques were selected, i.e., surface glossiness, measured non-destructively using a luster sensor (type CZ-H72 from Keyence Co., Osaka, Japan) and light reflection spectra, recorded by a spectrometer, whereas the three techniques fruit firmness (penetrometer), colorimeter (e.g., Minolta) and false colour images (profilometer) were excluded. (4) Results: The first technique based on fruit glossiness provides luster values depending on fruit colour. Green Bell pepper fruit with a Fresh Index in excess of 463–490 a.i. can be classified as fresh, and similarly of 525–565 a.i. for yellow and 486–502 a.i. for red pepper fruit. This first Freshness index dropped, indicating a lesser freshness, to less than 100 a.i. after 14 days storage, irrespective of fruit colour, thereby providing a suitable wide, ca. 5-fold, range of parameterization. The second index is based on the difference between the light reflectance peaks at 630–633 nm and at 500 nm, also measured perpendicular to the convex fruit side. These percentage values decreased from >40% with fresh red and yellow Bell pepper to ca. 20% after 14 days storage; similarly, these percentages decreased from ca. 16% to ca. 8% in green pepper fruit, in both cases by a twofold factor. Overall, a third Freshness index could be a combination of luster values larger than 470 a.u. and >40% difference of the light reflectance between 630 nm and 500 nm. (5) Conclusions: Two viz three freshness indices are proposed and thresholds elaborated for Bell pepper fruit. The analysis showed that both indices viz technologies, luster and light reflection spectra, require colour differentiation, i.e., a specific index for yellow, red and green Bell pepper fruit.
Unraveling Protein Interactions between the Temperate Virus Bam35 and Its Bacillus Host Using an Integrative Yeast Two Hybrid–High Throughput Sequencing Approach
Authors: Lechuga Ana , Lood Cédric , Berjón-Otero Mónica , del Prado Alicia , Wagemans Jeroen , van Noort Vera , Lavigne Rob , Salas Margarita , Redrejo-Rodríguez Modesto 
Published: October 2021

Bacillus virus Bam35 is the model Betatectivirus and member of the family Tectiviridae, which is composed of tailless, icosahedral, and membrane-containing bacteriophages. Interest in these viruses has greatly increased in recent years as they are thought to be an evolutionary link between diverse groups of prokaryotic and eukaryotic viruses. Additionally, betatectiviruses infect bacteria of the Bacillus cereus group, which are known for their applications in industry and notorious since it contains many pathogens. Here, we present the first protein–protein interactions (PPIs) network for a tectivirus–host system by studying the Bam35–Bacillus thuringiensis model using a novel approach that integrates the traditional yeast two-hybrid system and high-throughput sequencing (Y2H-HTS). We generated and thoroughly analyzed a genomic library of Bam35′s host B. thuringiensis HER1410 and screened interactions with all the viral proteins using different combinations of bait–prey couples. Initial analysis of the raw data enabled the identification of over 4000 candidate interactions, which were sequentially filtered to produce 182 high-confidence interactions that were defined as part of the core virus–host interactome. Overall, host metabolism proteins and peptidases were particularly enriched within the detected interactions, distinguishing this host–phage system from the other reported host–phage PPIs. Our approach also suggested biological roles for several Bam35 proteins of unknown function, including the membrane structural protein P25, which may be a viral hub with a role in host membrane modification during viral particle morphogenesis. This work resulted in a better understanding of the Bam35–B. thuringiensis interaction at the molecular level and holds great potential for the generalization of the Y2H-HTS approach for other virus–host models.
Enhanced Cellular Cryopreservation by Biopolymer-Associated Suppression of RhoA/ROCK Signaling Pathway
Authors: Lee Tae Wook , Lee Gyeong Won , An Seonyeong , Seong Keum-Yong , Lee Jong Soo , Yang Seung Yun 
Published: October 2021

With increasing demands on long-term storage of cells, cryopreservation of cells is gaining more importance in cell-based research and applications. Dimethyl sulfoxide (DMSO) is a commonly used chemical cryoprotectant, providing increased cell survival during the freezing process. However, its use is limited in clinical applications due to its low biocompatibility above cryogenic temperatures. Herein, we present a new approach for reducing the use of DMSO in cryopreservation by using biodegradable hyaluronic acids (HAs). By adding HAs into cryoprotectant media containing a low concentration of DMSO, higher cell viability and cell proliferation rate were observed upon thawing after cryopreservation. The HA-supplemented cryopreservation media did not reduce the size of the ice crystal, which significantly influenced cell viability during cell freezing, but decreased the Ras homolog family member A (RhoA)/Rho-associated protein kinase (ROCK) signaling pathway related to apoptosis. The cell-interactive cryoprotectants containing HA can be applied to the development of a new cryoprotectant that reduces the adverse effect of DMSO.
Clear Evidence of LAMA5 Gene Biallelic Truncating Variants Causing Infantile Nephrotic Syndrome
Authors: Taniguchi Yukimasa , Nagano China , Sekiguchi Kiyotoshi , Tashiro Atsushi , Sugawara Noriko , Sakaguchi Haruhide , Umeda Chisato , Aoto Yuya , Ishiko Shinya , Rossanti Rini , Sakakibara Nana , Horinouchi Tomoko , Yamamura Tomohiko , Kondo Atsushi , Nagai Sadayuki , Nagase Hiroaki , Iijima Kazumoto , Miner Jeffrey H. , Nozu Kandai 
Published: October 2021

Key Points

LAMA5 gene biallelic variants have been identified in only seven patients so far, and no functional analysis had been conducted for all but one.We report three patients with LAMA5 biallelic truncating variants manifesting infantile nephrotic syndrome and in vitro heterotrimer assays.We report one patient with SRNS with biallelic LAMA5 missense variants.

Background
Pathogenic variants in single genes encoding podocyte-associated proteins have been implicated in about 30% of steroid-resistant nephrotic syndrome (SRNS) patients in children. However, LAMA5 gene biallelic variants have been identified in only seven patients so far, and most are missense variants of unknown significance. Furthermore, no functional analysis had been conducted for all but one of these variants. Here, we report three patients with LAMA5 gene biallelic truncating variants manifesting infantile nephrotic syndrome, and one patient with SRNS with biallelic LAMA5 missense variants.

Methods
We conducted comprehensive gene screening of Japanese patients with severe proteinuria. With the use of targeted next-generation sequencing, 62 podocyte-related genes were screened in 407 unrelated patients with proteinuria. For the newly discovered LAMA5 variants, we conducted in vitro heterotrimer formation assays.

Results
Biallelic truncating variants in the LAMA5 gene (NM_005560) were detected in three patients from two families. All patients presented with proteinuria within 6 months of age. Patients 1 and 2 were siblings possessing a nonsense variant (c.9232C>T, p.[Arg3078*]) and a splice site variant (c.1282 + 1G>A) that led to exon 9 skipping and a frameshift. Patient 3 had a remarkable irregular contour of the glomerular basement membrane. She was subsequently found to have a nonsense variant (c.8185C>T, p.[Arg2720*]) and the same splice site variant in patients 1 and 2. By in vitro heterotrimer formation assays, both truncating variants produced smaller laminin α5 proteins that nevertheless formed trimers with laminin β1 and γ1 chains. Patient 4 showed SRNS at the age of 8 years, and carried compound heterozygous missense variants (c.1493C>T, p.[Ala498Val] and c.8399G>A, p.[Arg2800His]).

Conclusions
Our patients showed clear evidence of biallelic LAMA5 truncating variants causing infantile nephrotic syndrome. We also discerned the clinical and pathologic characteristics observed in LAMA5-related nephropathy. LAMA5 variant screening should be performed in patients with congenital/infantile nephrotic syndrome.
Pengembangan Bahan Ajar Materi Pythagoras yang Berorientasi pada Kompetensi Abad 21 untuk Guru SMP
Authors: Maryamah Ida , Anriani Nurul , Fathurrohman Maman 
Published: October 2021

Tujuan penelitian ini adalah untuk menghasilkan produk berupa buku ajar matematika yang berorientasi pada kompetensi abad 21 pada materi Pythagoras kelas VIII semester genap yang valid. Jenis penelitian ini merupakan penelitian pengembangan yang melipiuti tujuh langkah pokok, yaitu: analisis kebutuhan, kemampuan peneliti sebagai pengembang, produk yang dikembangkan, konsep produk, pengembangan produk, uji ahli, dan uji coba lapangan. Hasil penelitian berupa buku ajar matematika yang berorientasi pada kompetensi abad 21 pada materi Pythagoras kelas VIII yang valid. Kevalidan buku ajar diperoleh berdasarkan data dari angket penilaian para ahli, yaitu ahli materi, ahli bahasa, dan ahli media. Penilaian ahli materi mencapai kategori baik, ahli bahasa mencapai kategori sangat baik, dan ahli media mencapai kategori sangat baik. Sedangkan keterbacaan dan kepraktisan buku ajar diperoleh dari data angket penilaian guru matematika yang hasilnya mencapai kategori sangat baik. Jadi dapat disimpulkan bahwa buku ajar matematika yang berorientasi pada kompetensi abad 21 pada materi Pythagoras layak digunakan dalam kegiatan pembelajaran matematika. DOI: https://doi.org/10.31235/osf.io/9z27d
Pandemi COVID-19 Memberdayakan Ibu-Ibu yang kehilangan Pekerjaan untuk Mengelolah Sedekah Jum’at ke Mesjid dan Panti asuhan di Kota Makassar
Authors: Daga Rosnaini , Salam Karta Negara 
Published: October 2021

Pandemi COVID-19 di Indonesia pertama kali dideteksi pada tanggal 2 Maret 2020, ketika dua orang terkonfirmasi tertular dari seorang warga negara Jepang. Pada tanggal 9 April, pandemi sudah menyebar ke 34 provinsi dengan DKI Jakarta, Jawa Barat dan Jawa Tengah. Hingga 30 Juli 2021, Indonesia telah melaporkan 3.372.374 kasus positif menempati peringkat pertama terbanyak di Asia Tenggara. Dalam hal angka kematian, Indonesia menempati peringkat ketiga terbanyak di Asia dengan 92.311 kematian. Di tengah situasi pandemi seperti ini tentunya telah menimbulkan dampak yang sangat besar terhadap seluruh aspek, utamanya dalam aspek perekonomian. Komunitas Gebug Jum’at di Kota Makassar memiliki salah satu program yang bernama Jumat Berkah dimana kegiatan dilaksanakan setiap hari jumat berupa pembagian makanan baik itu makanan berat maupun makanan ringan kepada warga di Kota Makassar dengan tujuan sedekah dan menyambung silaturahmi antar warga setempat yang notabenenya warga menengah ke bawah karena kegiatan dilakukan secara bersama-sama.
Upaya Peningkatan Pengetahuan Ibu Hamil Melalui Pendidikan Kesehatan Mengenai Anemia Kehamilan
Authors: Retnaningtyas Erma , Yonni Siwi Retno Palupi , Wulandari Anggrawati , Fauzianty Reski , Muflikah Lilik , Putri Adela , Suharti Eti , Wahyuni Sri 
Published: October 2021

Anemia dalam kehamilan adalah kondisi ibu dengan kadar haemoglobin <11 gr% pada trimester I dan II, sedangkan pada trimester II kadar haemoglobin ibu hamil <10.5 gr%. Ibu hamil yang mengalami anemia beresiko menjalani persalinan yang abnormal dan kecenderungan pendarahan yang berdampak pada morbiditas dan mortalitas pada ibu dan bayi. Tujuan pengabdian masyarakat ini adalah untuk Meningkatkan pengetahuan ibu hamil tentang Anemia kehamilan. Kegiatan ini dilakukan dengan memberikan penyuluhan dengan leaflet kepada ibu hamil tentang anemia kehamilan dengan memberikan pre tes sebelum penyuluhan dan post tes sesudah penyuluhan. Penyuluhan ini dilaksanakan di Aula Desa Kota besi Hilir Kalimantan Tengah yang diikuti oleh 15 ibu hamil dengan menjalankan Protokol Kesehatan Covid 19. Hasil penyuluhan didapatkan Sebelum diberikan penyuluhan sebanyak 4 peserta (27%) dengan pengetahuan Baik dan setelah kegiatan terdapat peningkatan menjadi 12 peserta (80%) dengan penegtahuan Baik. Diharapkan untuk penyuluhan selanjutnya di fokuskan tentang Macam-macam anemia pada Ibu Hamil.
Обоснование параметров малогабаритной молотилки для обмолота початков кукурузы
Authors: Хажметова Залина Лиуановна , Шекихачев Юрий Ахметханович , Хажметов Лиуан Мухажевич , Шекихачева Людмила Зачиевна 
Published: October 2021

Одно из ведущих мест среди зерновых и кормовых культур занимает кукуруза. Из нее производят около 3500 видов продукции. Эта культура имеет большое значение как высоко энергетический корм для всех видов животных и птиц. В процессе производства зерна кукурузы наиболее трудоемким является сбор урожая – 60...80% от общих трудозатрат. В последнее наиболее трудоемким этапом сбора кукурузы является обмолот початков, качество которого характеризуется двумя основными показателями – травмированием зерна и недомолотом початков. На данный момент разработано большое количество молотилок различных по принципам и технологическими схемами обмолота. Существующие молотилки имеют большую производительность и предназначены для обмолота значительных объемов початков кукурузы. Однако в структуре выращивания кукурузы значительное место занимают небольшие посевы в частном секторе. После сбора початков кукурузы их обмолачивают вручную или изготавливают разнообразные ручные и механические молотилки. Кроме того, в настоящее время, когда еще недостаточно изучена физическая природа процесса обмолота молотилками кукурузы и недостаточно разработана их теория, распределение молотилок по принципу обмолота ударом или перетиранием является не полным. В связи с этим в статье обоснована конструктивно-технологическая схема малогабаритной молотилки, обеспечивающей обмолот початков кукурузы в обертке при минимальном травмировании зерен. В результате теоретических исследований установлены рациональные значения конструктивно-технологических параметров разработанного молотильного устройства: влажность початков 12…22%; частота вращения молотильного барабана 300…400 мин-1; высота зубьев 12…18 мм; шаг зубьев 3…8 мм..
Сдобное печенье повышенной пищевой ценности
Authors: Алексеенко Елена Викторовна , Белявская Ирина Георгиевна , Зайцева Лариса Валентиновна , Уварова Анна Георгиевна 
Published: October 2021

Приоритетным направлением в разработке новых видов сдобного печенья является модификация традиционных рецептур и использование инновационных ингредиентов, обеспечивающих повышение пищевой ценности готовых изделий и формирующих их функциональную направленность. Проведен комплекс исследований по экспериментальному обоснованию, разработке рецептуры и технологических решений с целью получения сдобного печенья повышенной пищевой ценности на основе муки пшеничной из цельносмолотого зерна и жирового продукта энзимной переэтерификации с омега-3 жирными кислотами с добавлением микроводоросли спирулины. Изучено влияние выбранных ингредиентов на органолептические, физико-химические показатели и пищевую ценность готовых изделий. Показано, что разработанный вид сдобного печенья имеет достойные потребительские свойства, по физико-химическим показателям - соответствует требованиям ГОСТ 24901-2014 при некотором снижении показателя намокаемости. Доказано, что разработанное изделие характеризуется улучшенным липидным профилем: в составе жирных кислот содержится значительное количество ненасыщенных жирных кислот (55%) по сравнению со сдобным печеньем, полученным по традиционной рецептуре, выявлено наличие γ-линоленовой кислоты, отмечается десятикратное увеличение содержания эссенциальной линолевой кислоты; печенье может быть позиционировано как изделие, являющееся источником незаменимых омега-3 жирных кислот (альфа-линоленовой более 0,2 г/100 г). Установлено, что сдобное печенье, полученное по усовершенствованной рецептуре, является источником пищевых волокон (5,87 г/100 г продукта), магния (20% средней суточной потребности на 100 г) и железа (23% средней суточной потребности на 100 г) при низком содержании натрия (соли) (42,3 мг/100г). Разработан комплект ТД (ТУ, ТИ) (проект) на новое обогащенное сдобное печенье.
Исследование физико-химического состава и технологических свойств овечьего и козьего молока в летний период лактации
Authors: Оспанов Асан Бекешович 
Published: October 2021

В статье представлены результаты исследования физико-химического состава и технологических свойств овечьего и козьего молока в летний период лактации (с июня по август 2021 года) по 4 породам овец (Казахский тонкорунный, Южноказахский меринос, Едильбай и Иль Де Франц) и 4 породам коз (Зааненская, Нубийская, Альпийская и Бурская) из различных регионов Казахстана. В связи с тем, что количество и качество молока в значительной мeрe зависят от корма и соответственно сезона года, было изучено изменение содержания жира и белка. Разница в содержании белка в летний период не превышает от 0,1 до 0,5 %, высокое содержание жира отмечалось в августе месяце (5,4% для овец и 3,6% для коз), а самое низкое в июле месяце (4,9 % для овец и 3,2 % для коз), это связано с климатическими условиями, в июле стоит жаркая погода и животные пьют больше воды и испытывают стрессы погодных аномалий а также летом основа рациона – пастбищный травостой, животные потребляют зеленый корм, содержащий много влаги. В августе значения показателей повышаются, так как многие кормовые культуры к этому времени созревают и питательная ценность соответственно возрастает.
Banimento e vigilância
Authors: Andreatta Samuel Medeiros 
Published: October 2021

Resumo
A evolução dos modelos de vigilância levou à criação de novos espaços de exclusão. O modelo banóptico é a lente aqui utilizada para verificar de que maneira o judiciário cria e sedimenta esses espaços nos crimes de associação ao tráfico no Superior Tribunal de Justiça (STJ). Foram coletadas e analisadas, dentro de uma perspectiva indutiva empírica sob um modelo banóptico, diversas sentenças em habeas corpus e os votos atinentes a elas. Concluiu-se que a jurisprudência tem atuado para fortalecer a exclusão por meio de uma associação “pura” de determinado tipo (o art. 35 da Lei de Drogas) aliada a um discurso sedimentado sobre determinado local, com forte centralidade no depoimento policial.
Palavras-chave: Criminologia Crítica- Surveillance- Política de Drogas- Direito Penal- Banóptico.