bluuebunny/crossref_metadata_2025_split huggingface dataset:
Patterns of regulatory divergence and gene expression in hybrids are associated with molecular evolution in species undergoing gene flow
Authors: Díaz Fernando , Wolf Jason , de Brito Reinaldo 
Published: November 2021

AbstractThe extent to which hybridization disrupts a gene’s pattern of expression likely governs its propensity for introgression, while its extent of molecular divergence can itself underlie such disruption. Together, these phenomena shape the landscape of sequence and transcriptional divergence across the genome as species diverge. To understand this process, we examine gene expression inheritance, regulatory and molecular divergences in the reproductive transcriptomes of species linked by gene flow. The fruit flies Anastrepha fraterculus and A. obliqua show evidence of gene flow despite clear evolutionary divergence and incomplete reproductive isolation. We find that their transcriptional patterns are a mosaic between those typically observed within and between allopatric species. Genes showing transgressive expression in hybrids or cis-regulatory divergence between species are associated with greater molecular divergence. This may reflect pleiotropic constraints that make them more resistant to gene flow or they may be more likely to experience divergent selection. However, while these highly divergent genes are likely to be important contributors to species differences, they are relatively rare. Instead, most differentially regulated genes, including those linked to reproduction, show high degrees of dominance in hybrids and trans-regulated divergence between species, suggesting widespread genetic compatibility that allowed for the identified introgression. These findings provide insights into how postzygotic isolating mechanisms might evolve in the presence of gene flow: regions showing cis-regulatory divergence or transgressive expression contribute to reproductive isolation, while regions with dominant expression and trans-regulatory divergence act as a buffer of hybrid breakdown, facilitating introgression, and leading to a genomic mosaic of expression and sequence divergence.
Ca2+-inactivation of the mammalian ryanodine receptor type 1 in a lipidic environment revealed by cryo-EM
Authors: Nayak Ashok R. , Samsó Montserrat 
Published: November 2021

AbstractActivation of the intracellular Ca2+channel ryanodine receptor (RyR) triggers a cytosolic Ca2+surge, while elevated cytosolic Ca2+inhibits the channel in a negative feedback mechanism. Cryo-EM carried out under partially inactivating Ca2+conditions revealed two conformations of RyR1, an open state and an inactivated state, resolved at 4.0 and 3.3 Å resolution, respectively. RyR1s were embedded in nanodiscs with two lipids resolved at each inter-subunit crevice. Ca2+binding to the high affinity site engages the central (CD) and C-terminal domains (CTD) into a quasi-rigid unit, which separates the S6 four-helix bundle and opens the channel. Further out-of-plane rotation of the quasi-rigid unit pushes S6 towards the central axis, closing (inactivating) the channel. The inactivated conformation is characterized by a downward conformation of the cytoplasmic assembly, a tightly-knit subunit interface contributed by a fully occupied and partially remodeled Ca2+activation site, and two salt bridges between the EF hand domain and the S2-S3 loop of the neighboring subunit validated by naturally-occurring diseasecausing mutations. Ca2+also bound to ATP, mediating a tighter interaction between S6 and CTD. Our study suggests that theclosed-inactivatedis a distinctive state of the RyR1 and its transition to theclosed-activablestate is not a simple reverse of the Ca2+mediated activation pathway.
pGlycoQuant with a deep residual network for precise and minuscule-missing-value quantitative glycoproteomics enabling the functional exploration of site-specific glycosylation
Authors: Cao Weiqian , Kong Siyuan , Zeng Wenfeng , Gong Pengyun , Jiang Biyun , Hou Xinhang , Zhang Yang , Zhao Huanhuan , Liu Mingqi , Qiao Xihua , Wu Mengxi , Yan Guoquan , Liu Chao , Yang Pengyuan 
Published: November 2021

AbstractInterpreting large-scale glycoproteomic data for intact glycopeptide identification has been tremendously advanced by software tools. However, software tools for quantitative analysis of intact glycopeptides remain lagging behind, which greatly hinders exploring the differential expression and functions of site-specific glycosylation in organisms. Here, we report pGlycoQuant, a generic software tool for accurate and convenient quantitative intact glycopeptide analysis, supporting both primary and tandem mass spectrometry quantitation for multiple quantitative strategies. pGlycoQuant enables intact glycopeptide quantitation with very low missing values via a deep residual network, thus greatly expanding the quantitative function of several powerful search engines, currently including pGlyco 2.0, pGlyco3, Byonic and MSFragger-Glyco. The pGlycoQuant-based site-specific N-glycoproteomic study conducted here quantifies 6435 intact N-glycopeptides in three hepatocellular carcinoma cell lines with different metastatic potentials and, together with in vitro molecular biology experiments, illustrates core fucosylation at site 979 of the L1 cell adhesion molecule (L1CAM) as a potential regulator of HCC metastasis. pGlycoQuant is freely available at https://github.com/expellir-arma/pGlycoQuant/releases/. We have demonstrated pGlycoQuant to be a powerful tool for the quantitative analysis of site-specific glycosylation and the exploration of potential glycosylation-related biomarker candidates, and we expect further applications in glycoproteomic studies.
Rotten web citations cited in scholarly journals: use of time travel for retrieval
Authors: Niveditha B. , Kumbar Mallinath , Sampath Kumar B.T. 
Published: November 2021

PurposeThe present study compares the use of web citations as references in leading scholarly journals in Library and Information Science (LIS) and Communication and Media Studies (CMS). A total of 20 journals (each 10 from LIS and CMS) were selected based on the publishing history and reputation published between 2008 and 2017.Design/methodology/approachThe present study compares the use of web citations as references in leading scholarly journals in LIS and CMS. A PHP script was used to crawl the Uniform Resource Locators (URLs) collected from the reference list. A total of 12,251 articles were downloaded and 555,428 references were extracted. Of the 555,428 references, 102,718 web citations were checked for their accessibility.FindingsThe research findings indicated that 76.90% URLs from LIS journals and 84.32% URLs from Communication and Media Studies journals were accessible and others were rotten. The majority of errors were due to HTTP 404 error code (not found) in both the disciplines. The study also tried to retrieve the rotten URLs through Time Travel, which revived 61.76% rotten URLs in LIS journal articles and 65.46% in CMS journal articles.Originality/valueThis is an in-depth and comprehensive comparative study on the availability of web citations in LIS and CMS journals articles spanning a period of 10 years. The findings of the study will be helpful to authors, publishers, and editorial staff to ensure that web citations will be accessible in the future.
Sales promotion: the role of equity sensitivity
Authors: Fam Kim-Shyan , Richard James E. , McNeill Lisa S. , Waller David S. , Zhang Honghong 
Published: November 2021

PurposeThis paper explores how consumer psychographics impact responses to sales promotions (SPs), and specifically whether equity sensitivity (ES) moderates attitudes towards sales promotion in the retail purchase experience (PE).Design/methodology/approachThe study examines data from a survey of 284 Hong Kong consumers, using a shopping mall-intercept method. Every third person walking past the researchers was asked to participate in the survey. After obtaining their permission, those agreeing to take part in the study were surveyed either inside or outside of the shopping complex. The face-to-face intercept surveying method also increases confidence in sample and response reliability.FindingsThe study finds that ES has a significant positive relationship with evaluations of the retail PE. Consumers identified as “Benevolents” were significantly more positive towards SPs and reported significantly higher satisfaction with the PE. In contrast, consumers identified as “Entitled” were less positive towards SP and less satisfied with the PE. In addition, noncash SPs significantly positively influenced Benevolents' PE.Research limitations/implicationsThe current study extends and expands equity theory and ES research by applying these concepts to consumer SPs. The study is limited to an examination of common consumer purchases, across different product categories and SP types. While this allows us to examine the relationship between SP attitudes, ES and purchase satisfaction, future comparisons between individual sales promotion techniques (SPTs) and specific consumer profiles are recommended.Practical implicationsFrom a retail perspective, it is important to understand individual differences and what influences and motivates the consumers' retail PE. Retail managers are advised to track customer purchases and satisfaction levels linked to SPs as this would allow for the identification of which customers are more likely to fit the Entitled or Benevolent psychological profiles and predict their likely responses to SP offers.Originality/valueTo date, there has been little research on individual psychological differences between consumers when offered SPs at retail stores. The current study contributes to the marketing literature by extending the price fairness equity model to the retail PE, thereby addressing a prominent gap in the literature.
Tourist's satisfaction with local food effect behavioral intention in COVID-19 pandemic: a moderated-mediated perspective
Authors: Rehman Shafique Ur , Samad Sarminah , Singh Sandeep , Usman Muhammad 
Published: November 2021

PurposeThis study explored the relationship between local food consumption value and satisfaction with local food, leading to behavioral intention. Moreover, tourist's involvement is used as a mediator, and COVID-19 fear moderates between satisfaction with local food and behavioral intention.Design/methodology/approachStructural equation modeling (SEM) technique presents researchers with extra flexibility and better research conclusions. This study used Partial Equation Modeling SEM to test the proposed hypotheses. The convenience sampling technique was used to collect data, and 339 questionnaires were part of the final analysis.FindingsThe results reveal that local food consumption value is positively associated with local food satisfaction except for emotional value. Satisfaction on local food significantly determined tourist's involvement and behavioral intention. Tourist's involvement is positively related to behavioral intention. Despite this, COVID-19 fear significantly decreases behavioral intention. Tourist's involvement significantly mediates, and COVID-19 fear moderates between satisfaction with local food and behavioral intention significantly.Practical implicationsThe results of our research will support scholars and practitioners to recognize the importance of factors that influence people's intention to eat local food. Besides, our research offers a significant policy to get maximum benefits for the tourism industry in Pakistan.Originality/valueTo the author's knowledge, our study initially incorporates a research model in the COVID-19 pandemic and covers local food consumption value, satisfaction on local food, tourist's involvement and COVID-19 fear to determine the behavioral intention of people to eat local food. Besides, consumption value theory was used to build a research framework.
Digital financial inclusion and vulnerability to poverty: evidence from Chinese rural households
Authors: Wang Xiuhua , Fu Yang 
Published: November 2021

PurposeDigital finance has the transformative power to realise financial inclusion. However, evidence on the relationship between digital finance and poverty reduction remains limited. This study examines the mitigating effects of digital financial inclusion (DFI) on vulnerability to poverty in rural China, explores potential mechanisms at the micro-level, and investigates the external conditions for DFI to validate these effects.Design/methodology/approachRural household data from the China Labour Force Dynamics Survey and the regional DFI index compiled by Peking University are used. The probit and mediation effect models are employed to assess the impacts of the DFI on vulnerability to poverty and explore its mechanisms, with an appropriate instrumental variable to mitigate potential endogeneity.FindingsDFI can mitigate vulnerability to poverty in Chinese rural households. Specifically, both sub-indices – coverage breadth and depth of use – have a significant effect. Further analyses based on the mediation model show that improving agricultural productivity, stimulating entrepreneurial activities and promoting non-agricultural employment are the core mechanisms for alleviating poverty vulnerability. Heterogeneity analysis shows that DFI is pro-poor and benefits those who lack economic opportunities. Moreover, adequate endowment in rural households, such as production and human capital, is an external condition for digital finance to mitigate vulnerability to poverty.Originality/valueThis study is among the first to examine the vulnerability-mitigation effects from the perspective of digital finance development, relying on data from a large-scale, nationwide household survey and the regional DFI index. It also checks for the mechanisms and heterogeneity of the effects, which prove the effects can help balance efficiency and equity.
Strategies for sustainable irrigation system management: a socio-technical system approach
Authors: Sirimewan DC , Samaraweera Aparna , Manjula NHC , Rameezdeen Raufdeen , Rodrigo MNN , Ekanayake EMAC 
Published: November 2021

PurposeIrrigation System Management (ISM) poses many issues in terms of social and technical aspects. These can be further elaborated as the issues toward the efficiency of infrastructure, equity of water sharing, environmental integrity and economic acceptability. Water losses and the structural changes of the system caused many issues putting the irrigation system into distress. Hence, this urges the need for sustainable ISM to improve social and technical attributes in ISM. This paper aims to apply the Socio-Technical Systems (STSs) theory for sustainable ISM.Design/methodology/approachThe qualitative expert opinion survey method was selected as the research strategy to approach the research aim. Sixteen industry professionals in the ISM sector were selected through the purposive sampling method to conduct semi-structured interviews to collect data in the Sri Lankan context. Code-based content analysis through a directed approach was used to analyse the data.FindingsFour main categories of strategies were emerged from the research findings as “engineering strategies”, “operational strategies”, “management strategies” and “regulatory strategies” through STS approach for sustainable ISM. The STS framework was developed by combining engineering and operational strategies into the technical subsystem and the management and regulatory strategies into the social subsystem to approach the aim of achieving contemporary system management in irrigation in a sustainable manner.Originality/valueThe research revealed the links between the strategies emerged from various disciplines to minimise the issues in ISM from the perspectives of technical and social subsystems. The study contributes to knowledge by providing a framework for sustainable ISM by applying the socio-technical systems theory by integrating the concepts of sustainability.
Psychosocial safety climate, work engagement and organizational commitment in Malaysian research universities: the mediating role of job resources
Authors: Gan Kia Hui , Kee Daisy Mui Hung 
Published: November 2021

Purpose
The current study is an examination of the effects of psychosocial safety climate on work engagement, organisational commitment and to mediate job resources in Malaysian research universities (RUs) during pandemic.

Design/methodology/approach
The population of this study consisted of full-time lecturers who work in Malaysian RUs at least a year. A sampling technique was used to select the respondents for this study. A total of 1,000 questionnaires were administered to respondents from 5 Malaysian RUs with 484 usable questionnaires were returned, giving a response rate of 48.4%.

Findings
The present study’s objective is to examine psychosocial safety climate’s (PSC) effect, job resources on work engagement and organizational commitment. The study also aims to test the mediating roles of job resources on PSC’s relationship, work engagement and organizational commitment. It is interesting to note that the relationship between PSC and work engagement was not significant.

Research limitations/implications
Although the present study had contributed to the existing literature, the present study’s result cannot be generalized. Suggestions for future research include an attempt to conduct a study over three-time points that looks at both the employee’s perspective, managerial perspective and organizational perspective within the workplace. All correlation and cross-sectional studies identified the need for a comprehensive three-wave study to examine the model’s longitudinal effects accurately.

Practical implications
The finding shown that university is suggested to apply higher PSC to allow their management discover more ways to increase the adequate job resources to support lecturers in RUs and in improving their work engagement and organizational commitment.

Originality/value
The integration of PSC in academicians of Malaysian RUs provides a novel perspective.
Use of 4.0 (I4.0) technology in HRM: a pathway toward SHRM 4.0 and HR performance
Authors: Pillai Rajasshrie , Yadav Shilpi , Sivathanu Brijesh , Kaushik Neeraj , Goel Pooja 
Published: November 2021

Purpose
This paper aims to investigate the use of Industry 4.0 (I4.0) technology and its barriers in human resourcemanagement (HRM) for Smart HR 4.0 and its impact on HR performance.

Design/methodology/approach
The research has been conducted using the grounded theory approach. Semi-structured interviews were conducted with 122 senior HR officers of national and multi-national companies in India after the extensive literature review. NVivo 8.0 software was used for the analysis of the interview data.

Findings
I4.0 technology is used for HRM functions by HR professionals. It is revealed that Smart HR 4.0 that emerged from the I4.0 technology has leveraged the HR performance. It is also found that usage barriers, traditional barriers and risk barriers affect the use of I4.0 technology in HRM.

Originality/value
A model is developed using the grounded theory approach for HR managers to understand the impact of I4.0 on HRM. This study reveals the barriers affecting the use of I4.0 technology in HRM. It also provides the model for HR performance that emerged through the use of I4.0 technology in HR and Smart HR 4.0. The research delivered key insights for the HR professionals, marketers of HR technology and technology developers.
Understanding social media users’ privacy-protection behaviors
Authors: Baker-Eveleth Lori , Stone Robert , Eveleth Daniel 
Published: November 2021

PurposeThis study aims to identify the roles that privacy experiences and social media use play in influencing privacy-protection behaviors. As social media use expands in terms of the number of users and functionality; it is important to understand social media user privacy-protection behaviors and the users’ psychological underpinnings driving those behaviors. Among these, perceptions are the users’ evaluation of their privacy concerns and data sharing benefits inherent in social media use which influence the users’ behaviors to protect their privacy.Design/methodology/approachTo research these issues, a theoretical model and hypotheses were developed, based on self-efficacy theory. The theoretical model was empirically tested using 193 questionnaire responses collected from students enrolled in business courses at a medium-sized university in the western USA. All the respondents reported that they routinely use social media. The empirical analysis was performed using structural equations modeling in PC SAS version 9.4, procedure Calis.FindingsThe estimation of the paths in the structural model indicates that privacy concerns positively influence social media users’ protection behaviors while the perceived benefits of data sharing negatively influence protection behaviors. Privacy experience positively influences privacy concerns. Alternatively, social media use positively influences social media self-efficacy and perceived usefulness, which, in turn, have meaningful influences on data sharing benefits.Originality/valuePrevious findings about the effect of self-efficacy on protection behaviors has been inconclusive. This study adds some clarity. Specifically, the findings suggest that the effect depends upon the foci of self-efficacy. While higher self-efficacy with respect to using privacy-related features of a specific technology tends to lead to greater privacy concerns, higher self-efficacy with respect to the more general technology (e.g. social media, computer) seems to affect protection behaviors through perceived benefits. Further, the results of this study offer conclusions about the roles that privacy experiences, social media use and perceived social media benefits play in affecting protection behaviors.
The transformative outcomes of frontline employee adaptability for service value co-creation: a study of the banking sector
Authors: Le Hau Nguyen , Pham Tram-Anh Ngoc , Pham Thuy Ngoc 
Published: November 2021

PurposeThis study aims to address two relatively unexplored issues in banking service literature. The first relates to the impact of co-creation behaviors of frontline employees (FLEs) on their well-being. The second is the impact of FLEs' adaptability on their performance of co-creation behaviors and their well-being in the workplace.Design/methodology/approachA structural model was built and tested using survey data collected from 366 FLEs offering financial consulting services to customers at banks.FindingsFLE co-creation behaviors have positive impacts on FLEs’ well-being, including well-being in the workplace (job satisfaction) and general well-being (quality of life). Moreover, FLEs with a high level of interpersonal and service-offering adaptability perform co-creation behaviors better than those with lower adaptability and have higher job satisfaction. Between service-offering adaptability and interpersonal adaptability, the former has stronger effects than the latter.Practical implicationsThe findings suggest that banks develop and enhance FLEs’ adaptability and co-creation behaviors to enhance their well-being and customer value.Originality/valuePrior research on FLEs' co-creation mainly focuses on customer-related transformative outcomes, leaving their own well-being less examined. This study fills this gap by providing evidence to suggest that although active co-creation behaviors require FLEs to have more skills and put in more effort, they do bring about transformative impacts in terms of better job satisfaction and quality of life. Additionally, a high level of adaptability helps FLEs to comfortably perform their co-creation behavior, thereby reducing stress and improving well-being.