bluuebunny/crossref_metadata_2025_split huggingface dataset:
Genome-Wide Analysis of Circular Rnas and Validation of hsa_circ_0086354 as a Promising Biomarker for Early Diagnosis of Cerebral Palsy
Authors: Hu Yuanyuan , Bian Xuzhao , Wu Chao , Wang Yan , Wu Yang , Gu Xiaoqin , Zhuo Suyan , Sun Shiquan 
Published: September 2021

Abstract
Background: Cerebral palsy (CP) is a spectrum of non-progressive motor disorders caused by brain injury during fetal or postnatal periods. Current diagnosis of CP mainly relies on neuroimaging and motor assessment. Here, we aimed to explore novel biomarkers for early diagnosis of CP. Methods: Blood plasma from five CP children and their healthy twin brothers/sisters was analyzed by gene microarray to screen out differentially expressed RNAs. Selected differentially expressed circular RNAs (circRNAs) were further validated using quantitative real-time PCR. Receiver operating characteristic (ROC) curve analysis was used to evaluate the value of using hsa_circ_0086354 as a biomarker of CP.Results: 43 up-regulated circRNAs and 2 down-regulated circRNAs were obtained by difference analysis (fold change>2, p<0.05), among which five circRNAs related to neuron differentiation and neurogenesis were chosen for further validation. Additional 30 pairs of CP children and healthy controls were recruited and five selected circRNAs were further detected, showing that hsa_circ_0086354 was significantly down-regulated in CP plasma compared with control, which was highly in accord with microarray analysis. ROC curve analysis showed that the area under curve (AUC) to discriminate CP children and healthy controls using hsa_circ_0086354 was 0.967, the sensitivity was 0.833 and the specificity was 0.966. Moreover, hsa_circ_0086354 was predicted as a competitive endogenous RNA for miR-181a, miR-4741 and miR-4656, and much literature evidence suggested that miR-181a may be a key target of hsa_circ_0086354 to regulate neuronal survival and neuronal differentiation. Conclusion: Hsa_circ_0086354 was significantly down-regulated in blood plasma of CP children, which may be a novel competent biomarker for early diagnosis of CP.
The Nonlinear Links Between Urbanization And CO2: Evidence From Unconditional Quantile And Threshold Regression
Authors: Kocoglu Mustafa , Awan Ashar , Tunc Ahmet , Aslan Alper 
Published: September 2021

Abstract
The extant literature has provided empirical evidences about the relationship between urbanization and environment, however, a little attention has been paid to non-linear relationship among them. This study aims to measure the effects of urbanization on carbon dioxide emission using quantile and threshold regression method. To this end, the study employed threshold analysis and quantile regression method and analyzed the variation of such non-linearity for different levels of carbon dioxide using quantile regression. The results illustrate that a single threshold and two regimes exist and the threshold for urbanization is 29.56%. Across both regimes, the elasticity estimates form an inverted U-shape impact of urbanization on the carbon dioxide emission. The increase in the marginal effect of urbanization on carbon dioxide emissions up to the median level and a declining trend after this level implies that environmental quality significantly improves for emerging country.
Monocyte Distribution Width As A Novel Sepsis Indicator in COVID-19 Patients
Authors: Alsuwaidi Laila , Heialy Saba Al , Shaikh Nahid , Najjar Firas Al , Seliem Rania , Han Aaron , Hachim Mahmood 
Published: September 2021

Abstract
Background
The severe acute respiratory syndrome coronavirus (SARS-CoV-2) is a highly transmittable virus which causes the novel coronavirus disease (COVID-19). Monocyte distribution width (MDW) is an in-vitro hematological parameter which describes the changes in monocyte size distribution and can indicate progression from localized infection to systemic infection. In this study we evaluated the correlation between the laboratory parameters and available clinical data in different quartiles of MDW to predict the progression and severity of COVID-19 infection.
Methods
A retrospective analysis of clinical data collected in the Emergency Department of Rashid Hospital Trauma Center-DHA from adult individuals tested for SARS-CoV-2 between January and June 2020. The patients (n = 2454) were assigned into quartiles based on their MDW value on admission. The four groups were analyzed to determine if MDW was an indicator to identify patients who are at increased risk for progression to sepsis.
Results
Our data showed a significant positive correlation between MDW and various laboratory parameters associated with SARS-CoV-2 infection. The study also revealed that MDW ≥ 24.685 has a strong correlation with poor prognosis of COVID-19.
Conclusions
Monitoring of monocytes provides a window into the systemic inflammation caused by infection and can aid in evaluating the progression and severity of COVID-19 infection.
Visualization of Radiocesium Distribution in Surface Layer of Seafloor Around Fukushima Daiichi Nuclear Power Plant
Authors: Sanada Yukihisa , Urabe Yoshimi , Misonou Toshiharu , Shiribiki Takehiko , Nakanishi Takahiro , Watanabe Yusuke , Tsuruta Tadahiko 
Published: September 2021

Abstract
Large quantities of volatile radionuclides were released into the atmosphere and the hydrosphere following the Fukushima Daiichi Nuclear Power Plant (FDNPP) accident on March 11, 2011. Monitoring of radiocesium in sediment is important for evaluating the behavior of radiocesium in the environment and its effect on aquatic organisms. In this study, the radiocesium distribution in the surface sediment around the FDNPP was visualized as a radiocesium map using periodical survey data from a towed gamma-ray detection system. The uncertainty of the radiocesium map was evaluated via comparison with a large amount of sediment core sample data. The characteristics of the radiocesium distribution were examined considering the seafloor topography and a geological map, which were obtained via acoustic wave survey. The characteristics of the formation of 137Cs anomaly at the estuaries were analyzed using a contour map of 137Cs concentration combined with water depth. Validation of the created map showed that it was comparable with actual sediment core samples. The map generated using the towed radiation survey depicted the 137Cs concentration distribution as the position resolution of a 1 km mesh. Finally, the 137Cs concentration decreased with time in consideration of such uncertainty.
The precision of gingival recession measurements is increased by an automated curvature analysis method
Authors: Kuralt Marko , Gašperšič Rok , Fidler Aleš 
Published: September 2021

Abstract
Background

The extent of gingival recession represents one of the most important measures determining outcome of periodontal plastic surgery. The accurate measurements are, thus, critical for optimal treatment planning and outcome evaluation. Present study aimed to introduce automated curvature-based digital gingival recession measurements, evaluate the agreement and reliability of manual measurements, and identify sources of manual variability.
Methods

Measurement of gingival recessions was performed manually by three examiners and automatically using curvature analysis on representative cross-sections (n = 60). Cemento-enamel junction (CEJ) and gingival margin (GM) measurement points selection was the only variable. Agreement and reliability of measurements were analysed using intra- and inter-examiner correlations and Bland-Altman plots. Measurement point selection variability was evaluated with manual point distance deviation from an automatic point. The effect of curvature on manual point selection was evaluated with scatter plots.
Results

Bland-Altman plots revealed a high variability of examiner's recession measurements indicated by high 95% limits of agreement range of approximately 1mm and several outliers beyond the limits of agreement. CEJ point selection was the main source of examiner's variability due to smaller curvature values than GM, i.e., median values of -0.98mm− 1 and − 4.39mm− 1, respectively, indicating straighter profile for CEJ point. Scatter plots revealed inverse relationship between curvature and examiner deviation for CEJ point, indicating a threshold curvature value around 1mm− 1.
Conclusions

Automated curvature-based approach increases the precision of recession measurements by reproducible measurement point selection. Proposed approach allows evaluation of teeth with indistinguishable CEJ that could be not be included in the previous studies.
Climate Change Scenarios, Their Impacts And Implications On Indian Cardamom-Coffee Hot Spots, One of The Two In The World
Authors: Muthusamy Murugan , Alagupalamuthirsolai M , Ashokkumar K , Anandhi A , Ravi R , Rajangam R , Dhanya M.K , Krishnamurthy K.S 
Published: September 2021

Abstract
In this study, we investigated climatic parameters as well as predicted future change in precipitation and atmospheric temperature levels based on RCP4.5/8.5 scenarios in all cardamom-coffee hot spots of southern India. Our results showed that larger precipitation levels and pattern occurred in Cardamom hills (Kerala) followed by lower Puleny hills in Tamil Nadu. The least variation in precipitation levels has noticed for temperate upper Puleny hills and Kodagu hills in Karnataka. RCP4.5/8.5 scenario analysis showed greater variability in precipitation up to 180% increase and 90% decrease for all hot sports. The scenario analysis also predicted extreme variation in temperature levels ranging from 0.5–8.5ºC increase for the entire study region. A significant change in the coffee yield and quality has been recorded over the last 30 years. Increased yield trends in coffee were noticed for Cardamom hills (CH) and Kodagu hills, but significantly lower coffee production was observed for lower Pulney hills. The mixed response of yield variability in coffee has been primarily attributed to the ongoing changing climatic factors. Ecophysiological studies of coffee, cardamom and black pepper have proved that coffee would adapt well to a future challenging climatic condition, closely followed by cardamom and black pepper. Since all the coffee-cardamom hot spots in southern India undergoes considerable change in precipitation levels and pattern, necessary precautions, including water and irrigation management strategies, must be given utmost priority to increase the crop yield sustainability of these delicate cardamom-coffee hot spots in India.
The Protective Effect of Carvacrol on Acetaminophen-Induced Renal Damage in Male Rats
Authors: Najafizadeh Alireza , Kaeidi Ayat , Rahmani Mohammadreza , Hakimizadeh Elham , Hassanshahi jalal 
Published: September 2021

Abstract
Acetaminophen overdose causes renal injury via oxidative stress and apoptosis induction. Carvacrol has antioxidant effect. The aim of this study was to determine the protective effect of carvacrol on acetaminophen-induced renal damage in male rats. In this experimental study, forty male Wistar rats were randomly divided to five groups (n = 8) including control, carvacrol 10 mg/kg, acetaminophen, acetaminophen + carvacrol 5 mg/kg, and acetaminophen + carvacrol 10 mg/kg. Animals initially received a single dose of acetaminophen (500 mg/kg), then were treated with carvacrol for one week (daily). Afterwards, renal blood flow (RBF), mean arterial pressure (MAP), renal perfusion pressure (RPP), renal vascular resistance (RVR), blood urea nitrogen (BUN), and serum creatinine were measured. Also, malondialdehyde (MDA) concentration, glutathione peroxidase (GPx) and superoxide dismutase (SOD) activity levels were measured in the kidney tissue. Hematoxylin and eosin method was used for histological assessment. The western blotting analysis was used to determine the Bax, Bcl-2 and cleaved caspase-3 proteins expression level in the kidney tissue. Carvacrol (10 mg/kg) could significantly increase the RBF, GPx and SOD activities and also reduced the RVR, serum creatinine, BUN, and MDA in the acetaminophen + carvacrol 10 mg/kg group versus acetaminophen group (P < 0.05). Also, carvacrol significantly decreased the cleaved caspase-3, Bax proteins expression level, and also kidney tissue damage score in the acetaminophen + carvacrol 10 mg/kg group versus acetaminophen group (P < 0.05). This study showed that carvacrol can attenuate the acetaminophen induced acute kidney damage via suppressing oxidative stress, apoptosis and its antioxidant effects.
Bioaccumulation And Biotransformation of BDE-47 Using Zebrafish Eleutheroembryos (Danio Rerio)
Authors: De Oro-Carretero Paloma , Landaluze Jon Sanz 
Published: September 2021

Abstract
Polybrominated diphenyl ethers (PBDEs) industrially used as flame retardants are nowadays considered emerging pollutants as they are endocrine disrupting chemicals (EDCs), persistent in the environment, bioaccumulative and in addition, its hydroxylated (OH-BDEs) and methoxylated (MeO-BDEs) metabolites have similar ecotoxic properties. The aim of this work was to develop an analytical method to be applied in the study of the bioconcentration and biotransformation of BDE-47 due to its bioavailability, toxicity and high persistence and abundance in environmental samples, including humans. So, a dependable ultrasonic extraction process followed to dispersive SPE clean-up step and GC-MS-μECD detection has worked out for the determination of BDE-47 and its main biotransformation products (MeO-BDEs and OH-BDEs), considering the polarity difference. In addition, an alternative method to bioconcentration official guideline OECD 305, developed previously with zebrafish (Danio rerio) eleutheroembryos (i.e., hatched but not yet free feeding embryos) is used, reducing dramatically the animal suffering but also time and reagents. Bioconcentration factors (BCF) were calculated using first order one-compartment toxicokinetic model. The profiles found show rapid absorption in the first hours of larval development and great bioaccumulative with capacity, finding bioconcentration factors (BCF) of 6631 and 44210 at nominal concentrations of 10 and 1 μg·L-1 (< 1% LC50), respectively. Metabolization studies show increasing concentrations of the metabolites BDE-28, 2'-OH-BDE-28 and 5-MeO-BDE-47 throughout the exposure time. The results obtained show the feasibility of the method for bioaccumulation and opens the possibility of metabolic studies with zebrafish eleutheroembryos, which is a very underdeveloped field without official testing or regulation.
A Survey of Residency Directors in Academic Trauma Centers for Policies on Airway Management in The Trauma Ward
Authors: Tewfik George , Gajewski Michal , Salem Jena , Borad Neil , Zales Michael , Govindraj Gopal 
Published: September 2021

Abstract
Background
Despite its presence as a critical procedure in the trauma setting, airway management is not performed uniformly, varying between institutions, particularly with personnel involved in decision-making. Past literature has noted a trend in which emergency medicine physicians assumed greater responsibility for primary management of airways in the trauma ward. In addition, many institutions have adopted tiered activation systems for traumas in order to improve patient care, deploying resources more effectively. In this study, a survey of residency directors was deployed to assess trends in airway management.
Methods
A validated survey was distributed to residency directors in anesthesiology, general surgery and emergency medicine in 190 Level I trauma centers in the United States. Questions assessed personnel management, complication tracking and difficult airway prediction factors, amongst other considerations for airway management in the trauma bay.
Results
Respondents completed the survey at a rate of 23.8% of those solicited. A majority of respondents indicated that emergency medicine physicians are primary airway managers in the trauma bay and that their institutions utilize tiered trauma activation systems at 77.4% and 95.6% respectively. Anesthesia providers were immediately available in 81% of respondent institutions with inconclusive data regarding protocols for delineating anesthesia involvement in difficult airways. More than a third of respondents indicated their institution either does not track airway complications or they did not know if complications were tracked. Finally, nine different criteria were used in varying degrees by respondents’ institutions to predict the presence of a difficult airway, including such factors as head/face trauma, airway fluid and obesity.
Conclusion
The trend towards airway management by emergency medicine physicians in the trauma bay continues, with anesthesia personnel available in many situations to assist in complicated patients. Complication tracking for airway management remains inconsistent, as does the criteria for prediction of the presence of difficult airways.
Correlation between the Accumulation of Skin Glycosylation End Products and the Development of type 2 Diabetic Peripheral Neuropathy
Authors: Zhao Xing-Wang , Yue Wan-Xu , Zhang Sen-Wei , Qiu Chen 
Published: September 2021

Abstract
With the increasing prevalence, disability and mortality of diabetic peripheral neuropathy (DPN), convenient, effective and inexpensive examination methods remain lacking. Advanced glycation end products (AGEs) have been shown to cause DPN independently or indirectly .Presently, the methods of detecting its concentration in serum are complicated and limited. AGE Pro developed by the Chinese Academy of Sciences uses the principle of positive correlation between fluorescence intensity of specific excitation/emission band and AGEs accumulation amount in skin tissues to invert the AGE level of subjects in vivo based on the obtained optical signals, to realize the risk and disease assessment of diabetes complications. Through the detection of skin AGEs with different severity of diabetic peripheral neuropathy and retrospective analysis of relevant data, this study showed that the accumulation of skin AGEs could reflect the severity of DPN to some extent. Skin AGEs were proven to be a good reference for the diagnosis and severity evaluation of DPN.
Understanding Satisfaction with Health care Services among Congolese Refugees in Bujumbura, Burundi
Authors: Misago Nadine , Habonimana Desire , Roberts Ann Alero , Bitangumutwenzi Patrick 
Published: September 2021

Abstract
Background3502 Congolese refugees based in Bujumbura possess the United Nations Higher Commissioner for Refugees (UNHCR) health insurance covering primary health care needs offered by CMC SOLIS clinic. This study sought to assess the quality of health care services received by Congolese refugees and determine factors affecting satisfaction. MethodsA cross-sectional survey was conducted on 400 refugees visiting CMC SOLIS for health care from June to August 2018. A questionnaire based on SERQUAL tool composed of five health quality dimensions was used to collect data on reliability, tangibility, assurance, responsiveness, and empathy. Logistic models were used to determine factors affecting satisfaction. ResultsOverall, 43% of refugees reported satisfaction with health care services. Age and gender significantly determined overall satisfaction. Other determinants such as education did not have significant effects. ConclusionsOverall, results from this study claim that CMC SOLIS does not offer quality health care services to Congolese refugees living in Bujumbura. Most importantly, the quality of health care services worsened with an increasing age as older ages were associated with less likelihood of satisfaction. Also, health care services offered to females seem to be deficient. Trial registration: N/A
Adaptive Coordinated Motion Constraint Control for Cooperative Multi-manipulator Systems
Authors: Su Chunjian , Zhang Min , Zhang Shuai , Guo Sumin , Wang Rui , Zhang Guosong , Yao Yanan , Zhang Qiang 
Published: September 2021

Abstract
A cooperative multi-manipulator system is a nonlinear, time-varying, strong coupling system with multiple inputs and outputs. Because of external disturbance, load change and other factors, a cooperative multi-manipulator system needs to address the problems of uncertain environment interaction, feasible control methods and coordinated planning. In this study, the coordinated motion of a multi-manipulator is divided into two types: coupling and superposition motions. An adaptive coordinated motion constraint scheme is proposed for the two different motion forms. The coupled and superposition motions were investigated by coordinating a carrying task and a circle drawing task, respectively, and the effectiveness of the adaptive coordinated motion scheme was verified. Based on the adaptive kinematics constraint algorithm of the multi-manipulator, co-simulation analysis of the multi-manipulator coordinated motion is conducted. Simulation results show that the multi-manipulator preserves the coordination relationship at all points of the trajectory planning path and has a good motion effect. Finally, an experimental platform for a cooperative multi-manipulator system was built to conduct experimental research on the coordinated motion of the multi-manipulator. Experimental results show that the proposed adaptive motion constraint control scheme for cooperative multi-manipulator systems has a good coordination effect, strong adaptivity and high-control precision.
Evaluating Effect of Metallic Ions on Aggregation Behavior of Amyloid Aβ42 by AFM Imaging and SERS
Authors: Xie Yang , Yu Lin , Fu Yuna , Sun Heng , Liu Chundong , Wang Jianhua 
Published: September 2021

Abstract
In this study, self-assembled monolayer (SAM) method was employed to fabricate monolayer of β-amyloid peptides Aβ42 on gold substrate with a bolaamphiphile named thiol (MHA). Firstly, the samples of gold substrate (blank control), the MHA-modified substrate and the Aβ42-modified substrate were detected by X-ray photoelectron spectroscopy (XPS) to track the self-assembly process. The spectra of binding energy measured from these three sample surfaces could be well fitted with the corresponding monolayer’s composition, which means Aβ42 monolayer is well formed. Aggregation behaviors of Aβ42 in the absence and presence of metallic ions (Zn2+、Ca2+、Al3+) were then monitored by atomic force microscopy (AFM) and surface-enhanced Raman Scattering (SERS), respectively. The recorded surface morphology of different experimental groups obtained by AFM showed markedly different nanostructures, indicating occurrence of aggregation behaviors of Aβ42. In solutions with added metal ions, the increased size of surface structures was observed, which suggest the presence of metal cations promotes aggregation behavior between Aβ42 molecules. Furtherly, the interaction between Aβ42 and metal cations was investigated by SERS. The results demonstrate that the Raman strength of Aβ42 changes after the metal cation treatment. Taken together, the combined AFM imaging and Raman analyses show that the three kinds of metallic ions promote the process of Aβ42 aggregation.