bluuebunny/crossref_metadata_2025_split huggingface dataset:
Quantitative imaging in whole-mount zebrafish embryos traces morphogen gradient maintenance and noise propagation in BMP signaling
Authors: Wang Xu , Li Linlin , Bu Ye , Liu Yixuan , Wu Tzu-Ching , Umulis David M. 
Published: November 2021

AbstractDorsoventral (DV) embryonic patterning relies on precisely controlled interpretation of morphogen signaling. In all vertebrates, DV axis specification is informed by gradients of Bone Morphogenetic Proteins (BMPs). We developed a 3D single-molecule mRNA quantification method in whole-mount zebrafish to quantify the inputs and outputs in this pathway. In combination with 3D computational modeling of zebrafish embryo development, data from this method revealed that Sizzled (Szl), shaped by BMP and Nodal signaling, kept a consistent inhibition level with Chordin (Chd) to maintain the BMP morphogen gradient. Intriguingly, BMP morphogen intrinsic expression is highly noisy at the ventral marginal layer in early zebrafish gastrula, where the gradient for DV patterning is established, which implies an unexpected role for noise in gradient shaping.
Convergent evolution of elaborate nests as structural defences in birds
Authors: Street Sally E. , Jaques Robert , De Silva Thilina N. 
Published: November 2021

AbstractThe ‘hanging-basket’ nests of some weaverbird and icterid species are among the most complex structures built by any animal, but why they have evolved remains to be explained. The precarious attachments and extended entrance tunnels characteristic of these nests are widely speculated to act as structural defences against invasion by nest predators, particularly tree-climbing snakes, but this hypothesis has yet to be systematically tested. We use phylogenetic comparative methods to investigate the role of nest structure in developmental period length, a proxy for offspring mortality, in the weaverbirds (Ploceidae) and icterids (Icteridae), two bird families in which ‘hanging-basket’ nests have independently evolved. We find that more elaborate nest designs, particularly those with entrance tunnels, are associated with longer developmental periods in both families. This finding is robust to potentially confounding effects of body mass, phylogenetic relationships and nest location. We provide the first comparative evidence for the role of elaborate nest designs in reducing offspring mortality from nest invasion. More broadly, our findings highlight the importance of offspring mortality risk in shaping diversity in birds’ nest designs and suggest that complex structures built by animals can buffer against environmental hazards and facilitate the evolution of slower life histories.
Reproductive isolation via polygenic local adaptation in sub-divided populations: effect of linkage disequilibria and drift
Authors: Sachdeva Himani 
Published: November 2021

AbstractThis paper considers how polygenic local adaptation and reproductive isolation between hybridizing populations is influenced by linkage disequilibria (LD) between loci, in scenarios where both gene flow and genetic drift counteract selection. It shows that the combined effects of multi-locus LD and genetic drift on allele frequencies at selected loci and on heterozygosity at neutral loci are predicted accurately by incorporating (deterministic) effective migration rates into the diffusion approximation (for selected loci) and into the structured coalescent (for neutral loci). Theoretical approximations are tested against individual-based simulations and used to investigate conditions for the maintenance of local adaptation on an island subject to one-way migration from a differently adapted mainland, and in an infinite-island population with two habitats under divergent selection. The analysis clarifies the conditions under which LD between sets of locally deleterious alleles allows these to be collectively eliminated despite drift, causing sharper and (under certain conditions) shifted migration thresholds for loss of adaptation. Local adaptation also has counter-intuitive effects on neutral (relative) divergence: FST is highest for a pair of subpopulations belonging to the same (rare) habitat, despite the lack of reproductive isolation between them.Author summaryEnvironmental adaptation often involves spatially heterogeneous selection at many genetic loci. Thus, the evolutionary consequences of hybridisation between populations adapted to different environments depend on the coupled dynamics of multiple loci under selection, migration and genetic drift, making them challenging to predict. Here, I introduce theoretical approximations that accurately capture the effect of such coupling on allele frequencies at individual loci, while also accounting for the stochastic effects of genetic drift. I then use these approximations to study hybridisation in a metapopulation consisting of many interconnected subpopulations, where each subpopulation belongs to one of two habitats under divergent selection. The analysis clarifies how subpopulations belonging to a rare habitat can maintain local adaptation despite high levels of migration if net selection against multi-locus genotypes is stronger than a threshold which depends on the relative abundances of the two habitats. Further, local adaptation in a metapopulation can significantly elevate FST between subpopulations belonging to the same habitat, even though these are not reproductively isolated. These findings highlight the importance of carefully considering the genetic architecture and spatial context of divergence when interpreting patterns of genomic differentiation between speciating populations.
Multiplex genomic recording of enhancer and signal transduction activity in mammalian cells
Authors: Chen Wei , Choi Junhong , Nathans Jenny F. , Agarwal Vikram , Martin Beth , Nichols Eva , Leith Anh , Lee Choli , Shendure Jay 
Published: November 2021

AbstractMeasurements of gene expression and signal transduction activity are conventionally performed with methods that require either the destruction or live imaging of a biological sample within the timeframe of interest. Here we demonstrate an alternative paradigm, termed ENGRAM (ENhancer-driven Genomic Recording of transcriptional Activity in Multiplex), in which the activity and dynamics of multiple transcriptional reporters are stably recorded to DNA. ENGRAM is based on the prime editing-mediated insertion of signal- or enhancer-specific barcodes to a genomically encoded recording unit. We show how this strategy can be used to concurrently genomically record the relative activity of at least hundreds of enhancers with high fidelity, sensitivity and reproducibility. Leveraging synthetic enhancers that are responsive to specific signal transduction pathways, we further demonstrate time- and concentration-dependent genomic recording of Wnt, NF-κB, and Tet-On activity. Finally, by coupling ENGRAM to sequential genome editing, we show how serially occurring molecular events can potentially be ordered. Looking forward, we envision that multiplex, ENGRAM-based recording of the strength, duration and order of enhancer and signal transduction activities has broad potential for application in functional genomics, developmental biology and neuroscience.
Ranking the Effectiveness of Non-Pharmaceutical Interventions to Counter COVID-19 in UK Universities with Vaccinated Population
Authors: Niu Zirui , Scarciotti Giordano 
Published: November 2021

AbstractSeveral universities around the world have resumed in-person teaching after successful vaccination campaigns have covered 70/80% of the population. In this study, we combine a new compartmental model with an optimal control formulation to discover, among different non-pharmaceutical interventions, the best prevention strategy to maximize on-campus activities while keeping spread under control. Composed of two interconnected Susceptible-Exposed-Infected-Quarantined-Recovered (SEIQR) structures, the model enables staff-to-staff infections, student-to-staff cross infections, student-to-student infections, and environment-to-individual infections. Then, we model input variables representing the implementation of different non-pharmaceutical interventions and formulate and solve optimal control problems for four desired scenarios: minimum number of cases, minimum intervention, minimum non-quarantine intervention, and minimum quarantine intervention. Our results reveal the particular significance of mask wearing and social distancing in universities with vaccinated population (with proportions according to UK data). The study also reveals that quarantining infected students has a higher importance than quarantining staff. In contrast, other measures such as environmental disinfection seems to be less important.
Development and quality assessment of low-sodium functional chevon patties
Authors: Nayak Narendra Kumar , Pathak Vikas 
Published: November 2021

PurposeThe study aims to develop low-sodium chevon patties from low-fat emulsion using various salt substitutes (KCl, CaCl2 and mushroom extract) in different combination without affecting the quality of the products.Design/methodology/approachEfficacy of salt substitutes was assessed for the development of low-sodium chevon patties. The developed products were assessed for various physicochemical properties, instrumental texture and color profile, estimation of mineral and sensory attributes.FindingsSignificant difference (p < 0.05) in mineral content was observed among the patties developed with different salt blends. Sodium content was reduced to the level of 38.07% in salt-substituted chevon patties. Calcium content was significantly (p < 0.05) higher in LS2, LS3 and LS4 salt-substituted chevon patties. Hardness, gumminess and chewiness values were significantly (p < 0.05) higher, and the springiness value was significantly (p < 0.05) lower in control chevon patties compared to treatments. Flavor and saltiness were significantly (p < 0.05) lower in LS1, LS3 and LS4 compared to control. However, flavor, juiciness and saltiness as well overall acceptability scores for LS2 were superior and widely accepted among the sensory panelists.Practical implicationsBased on sensory attributes and physicochemical properties, it is concluded that LS2 salt blend may suitably be used as excellent salt replacer to develop low-sodium chevon patties.Originality/valueInnovative findings of the experiment strengthen the current literature available on functional animal food products. Further, it provides one of the important natural alternatives to develop low-sodium meat products with special reference to chevon. Looking to increase attention toward health of the consumer and increase in the risk of cardiovascular diseases, the demand of low-sodium products is very high. Therefore, this may be the excellent choice without affecting the quality and sensory attribute of the products.
Valorisation of mango (Mangifera indica) kernel as an ingredient of macaron: sensory acceptance and physicochemical properties
Authors: Ng Seng Bee , Pong Ze Yee , Chang Kian Aun , Neo Yun Ping , Chew Lye Yee , Khoo Hock Eng , Kong Kin Weng 
Published: November 2021

PurposeMango seed, an agricultural food (agri-food) waste obtained during mango processing is generally discarded and causes environmental stress. In the present study, mango kernel was processed into flour and incorporated into macaron formulation. This study aimed to investigate the antioxidant potential, physicochemical properties, and sensory qualities of macaron after substitution of almond flour (AF) with 0% (control, MC-0), 20% (MC-20), 40% (MC-40), 60% (MC-60), 80% (MC-80) and 100% (MC-100) (w/w) of mango kernel flour (MKF).Design/methodology/approachSensory evaluation was conducted using a nine-point hedonic scale (n = 90 young adults) to evaluate the acceptance towards MC-0 (control), MC-20, MC-40, MC-60, MC-80 and MC-100 macaron shells (without mango-flavoured ganache). The most preferred formulated and control macarons were subjected to proximate analyses according to the methods of Association of Official Analytical Chemists for moisture, ash, and protein, and American Oil Chemists' Society for fat. Carbohydrate content was estimated by difference. Physical analyses such as colour, pH and water activity (aw) were performed using various instrumental techniques. Antioxidant activity of all macaron shell formulations was accessed using 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity (DPPH-RSA). Sensory evaluation was repeated using a five-point hedonic scale to determine the acceptance of the most preferred macaron (with mango-flavoured ganache) among consumer panels of all age groups (n = 80).FindingsThe first sensory acceptance test revealed that macaron shell with 40% MKF substitution (MC-40) was the most preferred formulation among young adults aged 18–35 years old. Moisture and ash contents between MC-0 and MC-40 were found to be similar (p > 0.05), while significant differences (p < 0.05) were observed for the fat and protein contents. Antioxidant activity increased proportionally with increasing MKF substitution and exhibited significant improvement (p < 0.05) in MC-40. Approximately 93% of the panellists expressed their liking towards MC-40 in the second sensory acceptance test. Overall, this study demonstrated that 40% MKF substitution in macaron improved its antioxidant performance without compromising consumers' acceptance.Originality/valueThis innovative research features the incorporation of MKF in the development of an economical and healthier macaron. The results constitute industrial relevant findings pertaining to its application feasibility and nutritional properties. This research has contributed knowledge to the existing literature as well as benefitted food manufacturers to develop value-added products that could meet the needs and expectations of interested parties, including consumers, governmental organisations, health-cautious advocates and healthcare providers.
Adaptation and parametrization of an iron loss model for rotating magnetization loci in NO electrical steel
Authors: Schauerte Benedikt , Nell Martin Marco , Brimmers Tim , Leuning Nora , Hameyer Kay 
Published: November 2021

Purpose
The magnetic characterization of electrical steel is typically examined by measurements under the condition of unidirectional sinusoidal flux density at different magnetization frequencies. A variety of iron loss models were developed and parametrized for these standardized unidirectional iron loss measurements. In the magnetic cross section of rotating electrical machines, the spatial magnetic flux density loci and with them the resulting iron losses vary significantly from these unidirectional cases. For a better recreation of the measured behavior extended iron loss models that consider the effects of rotational magnetization have to be developed and compared to the measured material behavior. The aim of this study is the adaptation, parametrization and validation of an iron loss model considering the spatial flux density loci is presented and validated with measurements of circular and elliptical magnetizations.

Design/methodology/approach
The proposed iron loss model allows the calculation and separation of the different iron loss components based on the measured iron loss for different spatial magnetization loci. The separation is performed in analogy to the conventional iron loss calculation approach designed for the recreation of the iron losses measured under unidirectional, one-dimensional measurements. The phenomenological behavior for rotating magnetization loci is considered by the formulation of the different iron loss components as a function of the maximum magnetic flux density Bm, axis ratio fAx, angle to the rolling direction (RD) θ and magnetization frequency f.

Findings
The proposed formulation for the calculation of rotating iron loss is able to recreate the complicated interdependencies between the different iron loss components and the respective spatial magnetic flux loci. The model can be easily implemented in the finite element analysis of rotating electrical machines, leading to good agreement between the theoretically expected behavior and the actual output of the iron loss calculation at different geometric locations in the magnetic cross section of rotating electrical machines.

Originality/value
Based on conventional one-dimensional iron loss separation approaches and previously performed extensions for rotational magnetization, the terms for the consideration of vectorial unidirectional, elliptical and circular flux density loci are adjusted and compared to the performed rotational measurement. The presented approach for the mathematical formulation of the iron loss model also allows the parametrization of the different iron loss components by unidirectional measurements performed in different directions to the RD on conventional one-dimensional measurement topologies such as the Epstein frames and single sheet testers.
Price-limit effectiveness: evidence from the Borsa Istanbul (BIST)
Authors: Aktas Osman Ulas , Kryzanowski Lawrence , Zhang Jie 
Published: November 2021

Purpose
This paper aims to analyze the impact of price-limit hits by hit type and when such hits start and stop using intraday trades and quotes at a one-second frequency for firms included in the BIST-50 index during the 13-months starting with March 2008. Like the recent COVID-19 period, this period includes the heightened stress in global financial markets in September 2008.

Design/methodology/approach
Using intra-day trades and quotes at a one-second frequency, the authors examine the market effects of price limits for firms included in the BIST-50 index during the global financial crisis. The authors compare the values of various metrics for 60 min centered on price-limit hit periods. The authors conduct robustness tests using auto regressive integrated moving average (ARIMA) models with trade-by-trade and with 3-min returns.

Findings
The findings are supportive of the following hypotheses: magnet price effects, greater informational asymmetric effects of market quality and each version of price discovery. Results are robust using samples differentiated by cross-listed status, same-day quotes instead of transaction prices and equidistant and trade-by-trade returns.

Originality/value
The authors use intraday data to reduce measurement error that is particularly pronounced when daily data are used to assess price limits that start and/or stop during a trading session. The authors contribute to the micro-structure literature by using ARIMA models with trade-by-trade and 3-min returns to alleviate some bias due to the autocorrelations in returns around price-limit hits in the presence of a magnet effect. The authors include some recent regulation changes in various countries to illustrate the importance of circuit breakers using price limits during COVID-19.
An investigation of the tensile characteristics of printed handsheets
Authors: Engin Merve , Sönmez Sinan , Kurt Mustafa Batuhan 
Published: November 2021

PurposeThe purpose of this paper is to investigate the influences of fibre lengths and a given range of paper grammages on the fundamental properties of unprinted and printed papers by using mineral oil-based offset printing inks and also evaluate these results in terms of printing and tensile characteristics.Design/methodology/approachA design research approach has been based on the production of various laboratory handmade papers and their printing process with mineral oil-based offset printing inks. The analysis of mechanical and structural tests results of the unprinted and the printed papers have been evaluated.FindingsThis study is confirmed that the mineral oil-based offset printing inks can be easily applied to the surface of papers having different grammages and pulp contents. An increase was observed in the tensile index values of the papers with the printing process, and these increases were more evident (about 80%) particularly in low grammage papers having high short fibre content.Originality/valueThe originality of this work is based on understanding and comparing the effects of grammage and the effect of pulp contents (having long and short fibre) on tensile characteristics of printed and unprinted handsheets.
Income changes do not influence political involvement in panel data from six countries
Authors: JUNGKUNZ SEBASTIAN , MARX PAUL 
Published: November 2021

AbstractThe income gradient in political participation is a widely accepted stylized fact. Based on nine panel datasets from six countries, this research note asks whether income changes trigger short‐term effects on political involvement. Irrespective of indicator, specification, and method (hybrid random effects models, fixed effects models with lags and leads, and error correction models), there are few significant short‐term effects of income changes. In conjunction with earlier research, this finding suggests that the income gradient in political participation is likely to reflect stable differences between rich and poor voters emerging early in the life course.
A tissue‐engineered, decellularized, connective tissue membrane for allogeneic arterial patch implantation
Authors: Yamanami Masashi , Kanda Keiichi , Morimoto Kazuki , Inoue Tomoya , Watanabe Taiji , Sakai Osamu , Kami Daisuke , Gojo Satoshi , Yaku Hitoshi 
Published: November 2021

AbstractBackgroundWe have previously applied in vivo tissue‐engineered vascular grafts constructed in patients’ subcutaneous spaces. However, since the formation of these vascular grafts depends on host health, their application is challenging in patients with suppressed regenerative ability. Therefore, the allogeneic implantation of grafts from healthy donors needs to be evaluated. This study aimed to fabricate allogeneic cardiovascular grafts in animals.Materials and methodsSilicone rod molds were implanted into subcutaneous pouches in dogs; the implants, along with surrounding connective tissues, were harvested after four weeks. Tubular connective tissues were decellularized and stored before they were cut open, trimmed to elliptical sheets, and implanted into the common carotid arteries of another dog as vascular patches (n = 6); these were resected and histologically evaluated at 1, 2, and 4 weeks after implantation.ResultsNo aneurysmal changes were observed by echocardiography. Histologically, we observed neointima formation on the luminal graft surface and graft wall cell infiltration. At 2 and 4 weeks after implantation, α‐SMA‐positive cells were observed in the neointima and graft wall. At 4 weeks after implantation, the endothelial lining was observed at the grafts’ luminal surfaces.ConclusionOur data suggest that decellularized connective tissue membranes can be prepared and stored for later use as allogeneic cardiovascular grafts.
The Geography and Education Research Group and school geography: Problematics and possibilities
Authors: Kinder Alan , Brace Steve 
Published: November 2021

AbstractThe project facing the Royal Geographical Society with Institute of British Geographers (RGS‐IBG) Geography and Education Research Group (GeogEd), launched at the RGS‐IBG annual conference in August 2019, is for the research group to explore points of connection between geographies of education and the scholarship of geography education, with its focus on geography in education and its practice of attending to questions of curriculum, pedagogy, teaching, learning, and progression. The purposes and scope of GeogEd are described in detail elsewhere in this issue. Suffice to say here that this is a broad and ambitious endeavour and we join others in wishing the research group well. This paper aims to make a contribution by identifying, from the perspectives of the Geographical Association (GA) and the RGS‐IBG, some of the intersections between the work of GeogEd and geography in schools and by shedding some light on the opportunities and challenges ahead. It concludes with proposals for new initiatives for the group to pursue.