bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effect of Gamma Radiation on the Adsorption Capacity and Regeneration / Desorption of Sulphate and Phosphate Ions Using Smart Hydrogel.
Authors: Eid Mona , Elarnaouty Magda , Hassouna Mohamed , Hafez Afaf , Salem Mohamed 
Published: December 2021

Abstract
Co-polymeric hydrogels containing poly (Acrylamide /Epichlorohydrine) P(AAm/EPI) with different acrylamide and Epichlorohydrine content were fabricated by gamma radiation at different irradiation doses as adsorbent materials for wastewater treatment. The mechanisms of radiation-induced crosslinking of hydrogel in aqueous solution has been evaluated. The gel contents and the swelling/diffusion kinetic parameters were evaluated at different irradiation doses, and the result confirm a non-fichian mechanism. The shape, surface morphology, and porosity of P (AAm/ Epi) hydrogel were characterized by scanning electron microscope (SEM). Adsorption experiments were carried out for the removal of sulphate and phosphate ions from wastewater using P(AAm/EPI) hydrogels as adsorbent materials. The isotherm data were analyzed by Freundlich equation. The equilibrium isotherm results show a better fitting (R2 > 0.9) to the Freundlich model for all anions. The calculated regeneration efficiency (%) values of sulphate and phosphate ions found to be ranged between 63.2 (%) and 46 (%).The relatively higher regeneration efficiency (%) and keeping the hydrogels its shape without any deformation promising to use the same hydrogels further times which decrease the economic cost.
Pulmonary Delivery of Engineered Exosomes to Suppress Postoperative Melanoma Lung Metastasis through Preventing Premetastatic Niche Formation
Authors: Han Xiaoqing , Bi Luopeng , Wu Yunyun , Yan Jiao , Wu Xiaqing , Zheng Runxiao , Sun yingying , Wang Yanbo , Zhang Haiyuan 
Published: December 2021

Abstract
Premetastatic niche (PMN) is a prerequisite for initiation of tumor metastasis. Targeting prevention of PMN formation in distant organs is becoming a promising strategy to suppress metastasis of primary tumor. Based on “organotropic metastasis”, melanoma tends to metastasize to lungs, where granulocytic myeloid-derived suppressor cells (G-MDSCs) recruitment in lungs significantly contributes to the PMN formation. Herein, functional exosomes (GExoI) were designed to present pulmonary targeting peptide GFE1 on the membrane and load PI3Kγ inhibitor (IPI549) inside, aiming at suppressing postoperative lung metastasis of melanoma. In postoperative mice model, intravenously injected GExoI could significantly accumulate in lungs and release IPI549 to block G-MDSCs recruitment through interfering with CXCLs/CXCR2/PI3Kγ signaling. The increased percentages of CD4+ T cells and CD8+ T cells in lungs could transform microenvironment from immunosuppression to immunostimulation, leading to metastasis inhibition. This study suggests an effective anti-metastasis strategy of targeting prevention of PMN formation through specifically blocking G-MDSCs recruitment.
A Standardized Approach to Orthotopic (Life-Supporting) Porcine Cardiac Xenotransplantation in a Non-Human Primate Model
Authors: Goerlich Corbin E. , Griffith Bartley P. , Treffalls John A. , Zhang Tianshu , Singh Avneesh K. , Lewis Billeta , Tatarov Ivan , Hershfeld Alena , Sentz Faith , Braileanu Gheorghe , Ayares David , Mohiuddin Muhammad M. 
Published: December 2021

Abstract
There are 5.7 million people in the United States with heart failure, which is life-limiting in 20% of patients.1 While data is most robust in the United States for this cohort, it is known to be a global problem with over 23 million people carrying the diagnosis.1 For end-stage heart failure, many require a heart transplantation, however, there is a shortage in the supply of organ donors. Cardiac xenotransplantation has been proposed to “bridge the gap” in supply for these patients requiring transplantation. Recent pre-clinical success using genetically modified pig donors in baboon recipients has demonstrated survival greater than 6 months.2–5 First-in-human transplantation of a genetically modified pig kidney demonstrated 54 hour rejection-free function when perfused by a deceased human recipient, demonstrating the feasibility of cross-species transplantation and invigorating enthusiasm further to utilize this new organ source for a population that would otherwise die waiting for a human organ.6 While this human study demonstrated proof-of-principle of overcoming hyperacute rejection, further regulatory oversight by Food and Drug Administration (FDA) may be required with pre-clinical trials in large animal models of xenotransplantation with long-term survival. These studies not only require a multi-disciplinary team and expertise in orthotopic transplantation (cardiac surgery, anesthesia and cardiopulmonary bypass), immunology and genetic engineering; but also, specifically handling large animal recipients that cannot communicate their symptoms. Here we detail our approach to pig-to-primate large animal model of orthotopic cardiac xenotransplantation perioperatively and in the months thereafter in long-term surviving animals. We also detail xenograft surveillance methods and common issues that arise in the postoperative period specific to this model and ways to overcome them.
Impairment of Neutrophil Functions and Homeostasis in COVID-19 Patients: Association With Disease Severity
Authors: Loyer Chloé , Lapostolle Arnaud , Urbina Tomas , Elabbadi Alexandre , Lavillegrand Jean-Rémi , Chaigneau Thomas , Simoes Coraly , Dessajan Julien , Desnos Cyrielle , Morin-Brureau Mélanie , Chantran Yannick , Aucouturier Pierre , Guidet Bertrand , Voiriot Guillaume , Ait-Oufella Hafid , Elbim Carole 
Published: December 2021

Abstract
Background Emerging data based on analyses of peripheral and pulmonary immune responses to SARS-CoV-2 increasingly suggest that a dysregulated immune response underpins the development of severe disease in COVID-19 patients. Neutrophils are key components of early innate immunity that, if not tightly regulated, contribute to uncontrolled systemic inflammation. We sought to decipher the role of neutrophil phenotypes, functions, and homeostasis in COVID-19 disease severity and outcome. Methods This longitudinal study compares study compares peripheral whole-blood neutrophils from 90 COVID-19 ICU patients with those of 22 SARS-CoV-2 – patients hospitalized for severe community-acquired pneumonia (CAP) and 38 healthy controls. We also assessed correlations between these phenotypic and functional indicators and markers of endothelial damage as well as disease severity. Results At ICU admission, the circulating neutrophils of the COVID-19 patients showed continuous basal hyperactivation not seen in CAP patients, associated with higher circulating levels of soluble E- and P-selectin, which reflect platelet and endothelial activation. Furthermore, COVID-19 patients had expanded aged-angiogenic and reverse transmigrated neutrophil subsets — both involved in endothelial dysfunction and vascular inflammation. Simultaneously, COVID-19 patients had significantly lower levels of neutrophil oxidative burst in response to bacterial formyl peptide, an abnormality that was greater in superinfected than non-superinfected COVID-19 patients. Moreover, patients dying of COVID-19 had significantly higher expansion of aged-angiogenic neutrophil subset and greater impairment of oxidative burst response than survivors. Conclusions These data suggest that neutrophil exhaustion may play a central role in the pathogenesis of severe COVID-19 and identify angiogenic neutrophils as a potentially harmful subset involved in fatal outcome.
Identification of the Yield Traits Related Haplotype Combinations of Transcription Factor Genes TaHDZ34 in Common Wheat
Authors: Yu Ming , Wang Xiaolong , Zhou Hongwei , Yu Yang , Wei Fan , Zhang Shuangxing , Song Tianqi , Wang Yukun , Zhang Xiaoke 
Published: December 2021

Abstract
The authors have requested that this preprint be removed from Research Square.
Influence of a Catalyst in Obtaining a Post-Consumer Pet-Based Alkyd Resin That Meets Circular Economy Principles
Authors: Senra Elaine Meireles , Silva Antônio E. F. A. da , Visconte Leila Lea Yuan , Pacheco Elen Beatriz Acordi Vasques 
Published: December 2021

Abstract
The paper studied the influence of a catalyst, comparing it with its traditional counterparts, in the process of obtaining a polyethylene terephthalate (PET)-based alkyd resin from post-consumer beverage bottles and how it consumes raw materials and generates waste. The resin was obtained in two phases: 1) glycerol and soybean oil alcoholysis reaction, a renewable material, for polyalcohol production, and 2) polyalcohol and polyacid esterification reaction to obtain the alkyd resin (reaction via solvent). A lithium octoate catalyst (OctLi) was used, not traditional in the alcoholysis reaction, and a fraction of the polyacid replaced by post-consumer PET at a proportion of up to 24% by weight in the esterification reaction. The OctLi catalyst caused a reaction in 30 min, compared to zinc acetate (120 min) and lithium hydroxide (LiOH, 60 min). Using post-consumer PET in obtaining the alkyd resin also decreased the esterification reaction time by 22% (8% PET), 67% (16% PET) and 72% (24% PET), compared to esterification without PET. The reaction time, considering alcoholysis with OctLi and partial esterification with PET (with 24% PET), was 180 min. Adding alcoholysis time with the LiOH catalyst and esterification without PET raises the reaction time to 600 min. Process water formed during the esterification stage declined by 15% (8% PET), 50% (16% PET) and 77% (24% PET), compared to the reaction without PET. The shorter reaction time resulted in less equipment use and consequent lower energy consumption. Another result was that the alkyd resin obtained with 8% PET was adequate for paint formulations.
Dynamic Performance-exposure Algorithm for Falling Risk Assessment and Fall Prevention in Community-dwelling Older Adults
Authors: Pereira Catarina , Rosado Hugo , Almeida Gabriela , Bravo Jorge 
Published: December 2021

Abstract
Background: Several models and algorithms were designed to identify older adults at risk of falling supported on an intrinsically and extrinsically traditional approach. However, the dynamic interaction between multiple risk factors for falls must be considered. The present study aimed to design a dynamic performance-exposure algorithm for falling risk assessment and fall prevention in community-dwelling older adults.Methods: The study involved 1) a cross-sectional survey assessing retrospective falls, performance-related risk factors for falls (sociodemographic such as gender and age, cognitive, health conditions, body composition, physical fitness, and dual-task outcomes), exposure risk factors (environmental hazards and (in)physical activity), and performance-exposure risk factors (affordance perception), and 2) follow-up survey assessing prospective falls. Participants were Portuguese community dwellings (≥ 65 years). Data were reported based upon descriptive statistics, curve estimation regression, binary logistic regression, and ROC curve.Results: The selected and ordered outcomes included in the algorithm and respective cutoffs were: (1) falls in previous year (high risk: n>1, moderated: n=1, low risk: n=0); (2) health conditions (high risk: n >3, moderated: n=3, low risk: n<3); (3) multidimensional balance (high risk: score <32 points, moderated risk: 32 points ≤ score ≤33 points, low risk: score>33); (4) lower body strength (high risk: rep/30s< 11, moderated risk: 11≤ rep/30s ≤14, low risk: rep/30s >4); (5) perceiving action boundaries (high risk: overestimation bias, moderated risk: not applied, low risk: underestimation bias); (6) fat body mass (high risk: % fat >38, moderated risk: 37≤ % fat ≤38, low risk: % fat <7); (7) environmental hazards (high risk: n>5, moderated risk: n=5, low risk: n<5); (8) rest period (high risk: hours/day >4.5, moderated risk: 4≤ hours/day ≤4.5, low risk: hours/day <4); (9) physical activity metabolic expenditure (high risk: MET-min/week <2300 or >5200, moderated risk: 2300≤ MET-min/week <2800, low risk: 2800≤ MET-min/week ≤5200).Conclusions: Results demonstrated a dynamic relationship between older adults’ performance capacity and the exposure to falls opportunity, supporting the build algorithm’s conceptual framework. Fall prevention measures should consider the above factors that most contribute to the individual risk of falling, relative weights, and their distance from low-risk value, as proposed in the dynamic algorithm.
TRAAC framework for regulatory acceptance and wider usability of tools and methods for safe innovation and sustainability of manufactured nanomaterials
Authors: Shandilya Neeraj , Barreau Marie-Sophie , Suarez-Merino Blanca , Porcari Andrea , Pimponi Daniela , Jensen Keld , Fransman Wouter , Franken Remy 
Published: December 2021

Abstract
There has been an unprecedented use of advanced materials, particularly manufactured nanomaterials, in industrial applications and consumer products across several sectors in the last two decades. It has instigated concerns about the sustainability, in particular, risks and uncertainties regarding the interactions of the manufactured nanomaterials with humans and the environment. Consequently, significant resources in Europe and beyond have been invested into the development of tools and methods to support risk mitigation and risk management, and thus facilitate the research and innovation process of the manufactured nanomaterials. The level of risk analysis is increasing, including assessment of socio-economic impacts, and sustainability aspects, moving from a conventional risk-based approach to a wider safety-and-sustainability-by-design perspective. Despite these efforts on tools and methods development, the level of awareness and use of the majority of such tools and methods by stakeholders is limited. Issues of user-friendliness, trust, implementation training, regulatory or authority compliance needs, regulatory acceptance and unsuitability to the users’ needs are some of the factors which have been traditionally known to hinder their widespread use. Therefore, a framework is presented to quantify the readiness of different tools and methods towards their wider regulatory acceptance and downstream use by different stakeholders. The framework diagnoses barriers which hinder regulatory acceptance and wider usability of a tool/method based on their Transparency, Reliability, Accessibility, Applicability and Completeness (TRAAC framework). Each TRAAC pillar consists of criteria which help in evaluating the overall quality of the tools and methods for their (i) compatibility with regulatory frameworks and, (ii) usefulness and usability for end-users, through a calculated TRAAC score based on the assessment. Fourteen tools and methods were assessed using the TRAAC framework as proof-of-concept. The results provide insights into any gaps, opportunities, and challenges in the context of each of the 5 pillars of the TRAAC framework.
Reliability Evaluation of CNC Machine Tools Considering Competing Failures of Fault Failure Data and Machining Accuracy Degradation Data
Authors: Feng Cong , Yang Zhaojun , Chen Chuanhai , Guo Jinyan , Leng Jiangong , Zhou Jie 
Published: December 2021

Abstract
Traditional reliability evaluation of CNC machine tools usually considers a single failure mode of fault failure or degradation failure, or considers fault failure and degradation failure to be independent of each other. However, in the actual working conditions, fault failure and degradation failure are mutually affected, and the reliability evaluation of the competing failure models of CNC machine tools by considering the two failure modes comprehensively can get more accurate evaluation results. Therefore, this paper proposes a reliability evaluation method for CNC machine tools considering fault failure data competing with machining accuracy degradation data. A fault failure model of CNC machine tools is established based on a non-homogeneous Poisson process. The fault failure model is updated according to the different effects of each maintenance result of the failure on machining accuracy. By integrating multiple geometric errors of CNC machine tools through multi-body system theory, the amount of machining accuracy degradation is extracted. A machining accuracy degradation failure model is established using the Wiener process. Considering the correlation between fault failure and degradation failure, a competing failure model based on the Coupla function is developed for evaluating the reliability of CNC machine tools. Finally, the effectiveness of the proposed method is verified by example analysis.
Perceptions of Community Health Workers (CHW) On Barriers And Enablers To Care For People With Psychosis In Rural Mozambique: Findings of a Focus Group Discussion Study Using The Capability, Opportunity, Motivation and Behavior Framework (COM-B Framework).
Authors: Mabunda Dirceu , Oliveira Déborah , Sidat Mohsin , Cournos Francine , Wainberg Milton , Mari Jair de Jesus 
Published: December 2021

Abstract
Background: Psychotic disorders contribute significantly to the global disease burden by causing disability, impaired quality of life, and higher mortality in affected people compared with the general population. In rural settings, where there is limited or no access to healthcare, individuals living with psychotic disorders often seek support from Community Health Workers (CHWs). However, little is known about what CHWs know about psychosis and how they manage such cases. This study aimed to explore the CHWs perception of psychosis and their experiences and beliefs about the factors that might enable or hinder care taking for patients with psychosis in rural settings in Mozambique. Methods: A qualitative study was conducted in rural districts of Maputo Province, a southern region of Mozambique, using six focus group discussions with participation of 79 CHWs. Thematic analysis was used informed by the Capabilities, Opportunities, Motivation and Behavior framework (COM-B).Results: Nine primary themes were identified. Overall, CHWs perceived psychosis as treatable medical conditions and held a positive attitude about being part of the care taking process of patients with psychosis in rural settings. Partnerships with key-stakeholders such as traditional healers, health care workers and families, were perceived by CHWs as enablers to improve access to care in rural areas. However, stigma, myths, and lack of competencies to treat people with psychosis were perceived by CHWs as barriers for appropriate care. Conclusion: CHWs, with adequate support, can play an important role in the care of patients with psychosis in rural settings, including identification of patients requiring care and referring them to appropriate healthcare professionals, and follow-up of medicated patients with psychosis within the rural remote setting. Training of CHWs should consider inclusion of basic mental health care competencies.
Morphological Changes in Blood Cells as Indicators for Disease Progression in COVID 19
Authors: A ABINAYA SUNDARI , M KARTHIKEYN T 
Published: December 2021

Abstract
A novel highly pathogenic human corona virus (COVID19) has been recently recognised in Wuhan, China as the cause of corona disease outbreak. It has rapidly spread from China to various countries across the world evolving as a pandemic. In our study we have categorized the covid positive patients into mild, moderate and severe based on the clinical criteria suggested by WHO. The coagulation parameters of the patients were analysed and documented. A peripheral smear was made for every patient and the morphological changes in blood cells were documented. The peripheral smear findings were then correlated with the disease stage and coagulation parameters. There were significant differences in the total WBC count and the differential WBC count between stages 1 &2 and stages 1 & 3 (p<0.005). Leucocytosis, neutrophilia and toxic changes in neutrophils were seen in severe stage of the disease and in covid coagulopathy suggesting these are important indicators of disease severity. Schistocytes an important finding in any other coagulopathy was not present in covid associated coagulopathy. Activated lymphocytes was found to be the most common morphological presentation seen in all covid patients irrespective of the disease stage whereas plasmacytoid lymphocytes was an important finding in severe stage disease. Monocyte cytoplasmic vacuoles, large/giant platelets were other morphological findings observed but these findings did not have any significant correlation with disease stage. Since follow up smears of the same patient were not analysed during disease progression and also post recovery, additional research in this field will provide further insights.
Oxygen Reduction Reaction Causes Iron Leaching from Fe-N-C Electrocatalysts
Authors: Ku Yu-Ping , Ehelebe Konrad , Bierling Markus , Speck Florian , Seeberger Dominik , Mayrhofer Karl , Thiele Simon , Cherevko Serhiy 
Published: December 2021

Abstract
The electrochemical activity of modern Fe-N-C electrocatalysts in alkaline media is on par with that of platinum. For successful application in fuel cells, however, also high durability and longevity must be demonstrated. Currently, design and synthesis of simultaneously active and stable platinum group metal-free electrocatalysts is hindered by a limited understanding of Fe-N-C degradation, especially under operando conditions. In this work, using a gas diffusion electrode half-cell coupled with inductively coupled plasma mass spectrometry setup, Fe dissolution is studied under more realistic conditions, i.e. real catalyst layer and current densities up to 125 mA·cm-2. Varying the rate of oxygen reduction reaction, we show a remarkable correlation between Faradaic electrode charge and Fe dissolution. This finding is rationalized assuming that oxygen reduction and Fe dissolution reactions are interlinked, likely through a common intermediate formed during the Fe3+/Fe2+ redox transitions in coordinated Fe cations. Moreover, such linear correlation allows an introduction and use of a simple metric (stability number). Hence, in the current work, a powerful tool for a more applied stability screening of different electrocatalysts is introduced, which allows on the one hand fast performance investigations under more realistic conditions, and on the other hand more advanced mechanistic understanding of Fe-N-C degradation in catalyst layers.