bluuebunny/crossref_metadata_2025_split huggingface dataset:
Tree line advance reduces mixing and oxygen concentrations in arctic–alpine lakes through wind sheltering and organic carbon supply
Authors: Klaus Marcus , Karlsson Jan , Seekell David 
Published: May 2021

AbstractOxygen depletion in lake bottom waters has adverse impacts on ecosystem health including decreased water quality from release of nutrients and reduced substances from sediments, and the reduction of fish growth and reproduction. Depletion occurs when oxygen is consumed during decomposition of organic matter, and oxygen replenishment is limited by water column stratification. Arctic–alpine lakes are often well mixed and oxygenated, but rapid climate change in these regions is an important driver of shifts in catchment vegetation that could affect the mixing and oxygen dynamics of lakes. Here, we analyze high‐resolution time series of dissolved oxygen concentration and temperature profiles in 40 Swedish arctic–alpine lakes across the tree line ecotone. The lakes stratified for 1−125 days, and during stratification, near‐bottom dissolved oxygen concentrations changed by −0.20 to +0.15 mg L−1 day−1, resulting in final concentrations of 1.1−15.5 mg L−1 at the end of the longest stratification period. Structural equation modeling revealed that lakes with taller shoreline vegetation relative to lake area had higher dissolved organic carbon concentrations and oxygen consumption rates, but also lower wind speeds and longer stratification periods, and ultimately, lower near‐bottom dissolved oxygen concentrations. We use an index of shoreline canopy height and lake area to predict variations among our study lakes in near‐bottom dissolved oxygen concentrations at the end of the longest stratification period (R2 = 0.41). Upscaling this relationship to 8392 Swedish arctic–alpine lakes revealed that near‐bottom dissolved oxygen concentrations drop below 3, 5, and 7 mg L−1 in 15%, 32%, and 53% of the lakes and that this proportion is sensitive (5%−22%, 13%−45%, and 29%−69%) to hypothetical tree line shifts observed in the past century or reconstructed for the Holocene (±200 m elevation; ±0.5° latitude). Assuming space‐for‐time substitution, we predict that tree line advance will decrease near‐bottom dissolved oxygen concentrations in many arctic–alpine lakes.
A tailored intervention for illness acceptance improves adherence and quality of life in adults with haemophilia using prophylaxis
Authors: Hoefnagels Johanna Wilhelmina , Fischer Kathelijn , Bos Ruud A. T. , Driessens Mariette H. E. , Schrijvers Liesbeth H. 
Published: May 2021

AbstractIntroductionAdherence to prophylactic treatment (prophylaxis) in persons with haemophilia is challenging and has been reported at only ±50%. Acceptance problems are one of the main reasons for non‐adherence in haemophilia. An evidence‐based intervention was developed based on an acceptance and commitment therapy (ACT) approach.AimTo evaluate a tailored intervention focused on illness acceptance in adults with haemophilia who were prescribed prophylaxis.MethodsA pre‐post study was executed in adults with haemophilia who were prescribed prophylaxis. A series of 8 2‐hour group trainings were held, including 3‐8 participants/series. Adherence (VERITAS‐Pro, optimum 0), health‐related quality of life (HRQoL, SF‐36, optimum 100) and illness perception (BIPQ, optimum 0) were measured at start, after six months and 12 months and analysed using Wilcoxon signed‐rank test.ResultsTwenty‐four patients (median age 47 years, range 27‐74) were included. After 12 months, adherence improved in 68% of patients, quality of life in 48% and illness perception in 31%. Adherence (total score) improved from 35 to 25 (P<0.01). HRQoL showed clinically relevant improvement in domains of social‐functioning (P = 0.04), role‐emotional, physical‐functioning, role‐physical and bodily pain. Illness perception improved statistically significant on domains of affect (P = 0.01), concern (P = 0.01) and understanding (P = 0.04). Patients evaluated the training useful, an eye‐opener, a personal enrichment and insightful.ConclusionThe tailored group intervention resulted in significant improvement of adherence, quality of life and illness perception. Based on our current experience, we have implemented it in clinical practice and collaborate with the patient association to make it available for all Dutch people with haemophilia.
NRAS Q61R immunohistochemical staining in thyroid pathology: sensitivity, specificity and utility
Authors: Saliba Maelle , Katabi Nora , Dogan Snjezana , Xu Bin , Ghossein Ronald A 
Published: May 2021

AimsThe diagnosis of thyroid neoplasms relies upon the demonstration of histological parameters that can be focal and prone to subjective interpretation. We evaluated the utility of NRAS Q61R immunohistochemistry (IHC) in the diagnosis of thyroid lesions after determining its specificity and sensitivity as a surrogate marker for RAS Q61R mutation.Method and resultsNRAS Q61R IHC was performed on 282 primary or metastatic thyroid lesions from 256 patients. RAS mutation status was collected from patients’ charts. Sensitivity and specificity of NRAS Q61R IHC for detecting a RAS Q61R mutation was calculated. IHC‐positive cases were reviewed to determine the diagnostic utility of NRAS Q61R IHC. NRAS Q61R immunopositivity was seen in non‐neoplastic, benign and malignant thyroid lesions. NRAS Q61R antibody cross‐reactivity led to the detection of NRAS Q61R, KRAS Q61R and HRAS Q61R proteins. Among primary thyroid carcinomas, immunopositivity was most frequent in papillary thyroid carcinomas, follicular variant (48.0%). The sensitivity and specificity of NRAS Q61R IHC in detecting RAS Q61R mutation was 90.6% and 92.3%, respectively. When positive, the NRAS Q61R stain was determined to be helpful in demonstrating infiltration, tumour size, capsular and/or vascular invasion and multifocality.ConclusionNRAS Q61R IHC is highly sensitive and specific for the detection of RAS Q61R mutations in thyroid pathology and is particularly relevant in follicular‐patterned neoplasms. When evaluated alongside histological features, NRAS Q61R immunoreactivity can be instrumental in the diagnosis and classification of thyroid nodules.
Shorebird embryos exhibit anti‐predator responses
Authors: Kostoglou Kristal N. , van Dongen Wouter F. D. , Bowe Steven J. , Weston Michael A. 
Published: May 2021

Ground‐nesting shorebirds rely on egg crypsis as one of their main anti‐predator defence strategies. Avian embryos vocalize late in incubation and communicate with parents through the eggshell but calls may compromise crypsis. Conceivably, embryos may cease calling when exposed to signals that suggest a predator is nearby (acoustic crypsis), but this has not been studied. Signals of nearby predators may include bird calls (predator, parental alarm), parental heart rate (higher when predators are nearby), or changes in light and temperature (incubators leave nests when predators are nearby). Experiments using eggs of Red‐capped Plovers Charadrius ruficapillus and Masked Lapwings Vanellus miles examined whether the number of embryonic vocalizations varied with exposure to the above factors. For both species, exposure to predator calls caused fewer embryonic calls (although this effect was evident for only one variant of predator calls for Red‐capped Plover). For Red‐capped Plovers, there was a statistically non‐significant tendency (P = 0.068) for exposure to light/changed temperature and noise to be associated with fewer calls. Parental heart rate (tested only in Lapwings) and exposure to light/temperature and noise change had no influence on the number of embryonic vocalizations for Masked Lapwings. We show that embryos are able to respond to predator calls and possibly light/temperature/noise levels as cues, in a manner consistent with anti‐predator behaviour before hatching.
Out of sight, out of mind: public and research interest in insects is negatively correlated with their conservation status
Authors: Wang Zhengyang , Zeng Jimmy , Meng Weilin , Lohman David J. , Pierce Naomi E. 
Published: May 2021

Abstract
Conservation ‘culturomics’ analyses changes in word frequencies within large digital sources to gain conservation insights. Studies of mammals and birds have found little correlation between conservation need and research effort or popular interest. In this study, we examine research and public interest in 9355 potentially endangered insect species from the Union for Conservation of Nature (IUCN) Red List.
Using search counts from Google search, Google Scholar and Ngram as indicators of public and research interest (Internet salience), we found that Internet attention is negatively correlated with the level of threat faced by an insect species. All measures of Internet salience and research effort were highly correlated, and searches for an insect's scientific name were more likely if it had a common name.
Since few past studies have incorporated phylogenetic information into their analyses, we used search counts and Twitter text content to study the phylogenetic signal of research interest across 870 insect families. Phylogenetic regressions demonstrate that species‐rich families receive more searches, but research interest is not proportional to the size of a family. Phylogenetically distinct insect families receive fewer searches per family and per species.
Our results suggest that many endangered insects, unlike vertebrates, have been largely ignored on the World Wide Web.
A replacement of traditional insulation refractory brick by a waste‐derived lightweight refractory castable
Authors: Hossain Sk S. , Bae Chang‐Jun , Roy P. K. 
Published: May 2021

AbstractLightweight insulation refractories are essential for high‐temperature performance to reduce energy consumption. This study investigates a new insulation material, that is, solid waste rice husk ash (RHA) derived lightweight refractory castable, replacing traditional insulation refractory brick. The RHA is generated after the burning of rice husk as biomass fuel. The RHA is used as an aggregate and alkali‐extracted silica sol from RHA as a binder to fabricate the insulation castable. The nanosilica containing (~30 wt%) sol is employed to synthesize the refractory castable by varying the sol amount (2.5‐12.5 wt% silica from sol). The castable specimens are cast by a vibro‐caster and fired at 900‐1200°C in a muffle furnace. The physic‐mechanical and thermal conductivity (κ) of the castable is investigated. At 1100°C with 10 wt% dry sol retaining sample shows an excellent apparent porosity (~65%), low bulk density (~ 0.8 g/cm3), and κ (0.136 W/m k) with sustainable compressive strength (6 MPa). The acquired results are a good match with the literature (other wastes‐derived insulation materials) and industrial (silica insulation brick) obtained data. These promising outcomes may inspire the refractory industries for using RHA as an aggregate and RHA extracted sol as a binder for making insulation castable.
A comparative study of the expectations of SME employers recruiting young people in Germany, Australia and the United States
Authors: Zenner‐Höffkes Lea , Harris Roger , Zirkle Chris , Pilz Matthias 
Published: May 2021

AbstractDebate continues to feature prominently in many countries on such notions as skills, skill shortages and employability. Clearly such notions remain significant internationally in the rhetoric of both economic reform and training policy and practice. However, the problem remains of differing perceptions. The purpose of this comparative study was to examine the expectations of small and medium‐sized enterprises (SMEs) when they recruit young people. Focusing on Germany, Australia and the United States, interviews were held with 30 managers in two diverse job roles—hospitality front‐desk staff and automotive technicians. In addition to differences in the range of desired skills between the job roles in these countries, difficulties in recruiting young people were highlighted by the study. Generational differences and academization were perceived as having effects on the hiring of young workers. Various problems with each country's general education system were also emphasized, as managers perceived many young people being inadequately prepared for work. The study also demonstrates the influence of the skill formation system of the countries is less relevant in comparison to the sector in which the SMEs are active.
COVID‐19 and future pandemics: is isolation and social distancing the new norm?
Authors: Collignon Peter 
Published: May 2021

AbstractThe coronavirus, named SARS‐CoV‐2, is the cause of COVID‐19. This virus spreads readily from person to person and predominantly to and from the respiratory route and through droplets. There are many different interventions that can be and are used to decrease successfully the risk and spread of COVID‐19. Most of the principles underpinning these interventions relate to isolation and social distancing. These will need to be continued, at least in part, until safe and very effective vaccines become widely available and are delivered extensively and successfully globally. This new norm is isolation, plus social and physical distancing, and this new norm will likely be with us for some time to come. It will also be with us in any future pandemics, whether caused by bacteria or viruses, but especially when the causative pathogen spreads predominantly through the respiratory route. However, lockdowns and restrictions also cause many adverse but unintended economic, social and health consequences. Therefore, what is put into place needs to be proportionate to levels of risk of disease as well as spread, and which will vary in different localities and with time.
Regulation of CTLA‐4 recycling by LRBA and Rab11
Authors: Janman Daniel , Hinze Claudia , Kennedy Alan , Halliday Neil , Waters Erin , Williams Cayman , Rowshanravan Behzad , Hou Tie Zheng , Minogue Shane , Qureshi Omar S. , Sansom David M. 
Published: May 2021

AbstractCTLA‐4 is an essential regulator of T‐cell immune responses whose intracellular trafficking is a hallmark of its expression. Defects in CTLA‐4 trafficking due to LRBA deficiency cause profound autoimmunity in humans. CTLA‐4 rapidly internalizes via a clathrin‐dependent pathway followed by poorly characterized recycling and degradation fates. Here, we explore the impact of manipulating Rab GTPases and LRBA on CTLA‐4 expression to determine how these proteins affect CTLA‐4 trafficking. We observe that CTLA‐4 is distributed across several compartments marked by Rab5, Rab7 and Rab11 in both HeLa and Jurkat cells. Dominant negative (DN) inhibition of Rab5 resulted in increased surface CTLA‐4 expression and reduced internalization and degradation. We also observed that constitutively active (CA) Rab11 increased, whereas DN Rab11 decreased CTLA‐4 surface expression via an impact on CTLA‐4 recycling, indicating CTLA‐4 shares similarities with other recycling receptors such as EGFR. Additionally, we studied the impact of manipulating both LRBA and Rab11 on CTLA‐4 trafficking. In Jurkat cells, LRBA deficiency was associated with markedly impaired CTLA‐4 recycling and increased degradation that could not be corrected by expressing CA Rab11. Moreover LRBA deficiency reduced CTLA‐4 colocalization with Rab11, suggesting that LRBA is upstream of Rab11. These results show that LRBA is required for effective CTLA‐4 recycling by delivering CTLA‐4 to Rab11 recycling compartments, and in its absence, CTLA‐4 fails to recycle and undergoes degradation.
Epidemiology and outcome of influenza‐associated infections among hospitalized patients with acute respiratory infections, Egypt national surveillance system, 2016‐2019
Authors: Fahim Manal , AbdElGawad Basma , Hassan Hossam , Naguib Amel , Ahmed ElSabbah , Afifi Salma , Abu ElSood Hanaa , Mohsen Amira 
Published: May 2021

AbstractIntroductionEgypt has established different types of surveillance systems to monitor influenza activities, early detect outbreaks, and tailor efficient prevention and control strategies. This is the first study to describe epidemiology and outcome of influenza‐associated infections among hospitalized patients using the National Electronic Disease Surveillance System (NEDSS) data, 2016‐2019.MethodsData reported from 284 hospitals all over Egypt were extracted from the NEDSS. Data of hospitalized patients with Acute Respiratory Infections (ARI), 2016‐2019, were included in the analysis. Laboratory testing for influenza by RT‐PCR according to US CDC testing protocol was used to confirm influenza type and subtype.ResultsOverall 46 417 patients hospitalized with ARI were identified, their mean age was 30.9 ± 26 and 52.9% were males. Among 41 512 (89.4%) laboratory‐tested patients, 7167 (17.3%) were positive for one or more types of influenza viruses. Influenza viruses circulated in all ages and throughout the year, with higher rates in winter, late childhood, and middle ages. Mortality from influenza was significantly higher than other causes of ARIs (5.0% vs 3.8%, P < .001), and it was associated with older ages, December‐May, delay in hospital admission, residence in urban and frontier governorates and infection with A/H1N1 virus. The distribution of influenza subtype by time shows alternate pattern between A/H1N1 and H3N2, each subtype peaks every other year with a high peak of A/H1N1 in 2016.ConclusionsThe national Egyptian surveillance succeeded to describe the epidemiology of hospitalized patients with ARIs and influenza in Egypt over time. Surveillance with strain‐specific laboratory testing and annual assessment of associated severity might be useful to guide influenza prevention and control strategies including vaccination and case management.
A pragmatic randomised controlled clinical study to evaluate the use of silicone dressings for the treatment of skin tears
Authors: LeBlanc Kimberly , Woo Kevin 
Published: May 2021

AbstractOne of the most common types of skin breakdown in ageing populations is skin tears. The International Skin Tear Advisory Panel advocates for special attention to be paid to dressing selection related to skin tear management. The panel recommends choosing dressings that will promote the maintenance of moisture balance, suit the local wound environment, protect peri‐wound skin, control or manage exudate and infection, and optimise caregiver time. It is paramount that dressings protect the fragile nature of the skin associated with those who at heightened risk for skin tear development. To compare the effectiveness of soft silicone dressings (a contact layer and/or foam) for the healing of skin tears with local practices that do not include soft silicone dressings. The study was a pragmatic randomised controlled prospective study. One hundred and twenty‐six individuals from two long‐term care facilities in Ontario Canada who presented with skin tears were randomised into the treatment group using either soft silicone dressings (a contact layer and/or foam) or the control group using non‐adhesive dressings. The current study demonstrated that 96.9% (n = 63) of skin tears in the treatment group healed over a 3‐week period compared with 34.4% (n = 21) in the control group. The proportion of wound healing experienced at week 2 was 89.2% (n = 58) in the treatment group compared with 27.9% (n = 17) in the control group. There was a significantly greater reduction in wound surface area relative to baseline in the treatment group (2.9 cm2) compared with the control group (0.6 cm2) (χ2 = 21.792 P < .0001) at week 1. Survival analysis data supported that skin tears healed 50% faster in the treatment group (11 days) compared with the control group (22 days) (χ2 = 59.677 P < .0001). The expected healing trajectory of acute wounds, including skin tears, if proper wound bed preparation is realised and infection is controlled, is 7 to 21 days. Results of this study suggest the use of silicone dressings support wound healing and aid in wound closure within the expected healing trajectory, with faster complete wound closure and mean healing times compared with non‐silicone dressing for the treatment of STs.
Strong differences in migratory connectivity patterns among species of Neotropical‐Nearctic migratory birds revealed by combining stable isotopes and abundance in a Bayesian assignment analysis
Authors: Studds Colin E. , Wunderle Joseph M. , Marra Peter P. 
Published: May 2021

AbstractAimConservation planning for migratory species requires knowing how populations are connected throughout the annual cycle. We measured migratory connectivity for five migratory songbird species by combining stable‐hydrogen isotopes in feathers (δ2HF) with citizen science data on breeding range abundance.LocationCaribbean Basin and North America.TaxaNeotropical migratory birds: Mniotilta varia, Setophaga ruticilla, Seiurus aurocapilla, Setophaga americana, and Setophaga discolor.MethodsWe analyzed δ2HF in feathers grown on North American breeding grounds and sampled at 20 Caribbean Basin sites across most of the non‐breeding range of each species. We made Bayesian assignments to breeding origin by combining δ2HF values and North American Breeding Bird Survey abundance data. For each species, we used cluster analysis to group Caribbean sites into non‐breeding regions that shared birds with similar breeding ground assignment probabilities. We then mapped assignment probabilities for the non‐breeding regions of each species onto breeding ground δ2HF surfaces and estimated the strength of migratory connectivity.ResultsApart from some general similarities, the five species demonstrated different patterns of migratory connectivity. Species with larger non‐breeding ranges exhibited stronger migratory connectivity compared to those with smaller non‐breeding ranges. Despite geographical overlap, birds from each non‐breeding region had mostly unique breeding ground origins.Main conclusionsThe contrasting patterns of migratory connectivity shown by the five species suggest that geographic distribution during the non‐breeding period is a poor indicator of breeding ground origin. Our results indicate that full annual cycle conservation planning, which requires migratory connectivity information, should proceed on a species‐specific basis.
Age‐related alterations in cardiac and arterial structure and function in hypertensive women and men
Authors: Cai Anping , Zhou Dan , Liu Lin , Zhou Yingling , Tang Songtao , Feng Yingqing 
Published: May 2021

AbstractThe study was to compare age‐related alterations in cardiac and arterial structure and function by sex and to explore the impacts of achieved systolic blood pressure (SBP; <130 mm Hg vs. <140 mm Hg) level on age‐related alterations in cardiac and arterial structure and function in hypertensive women and men. Community hypertensive individuals without cardiovascular disease who had echocardiographic examination were included. Age‐related alterations in cardiac and arterial structure and function were compared by sex, and interplay between age and sex was analyzed according to achieved SBP level. The mean age of the cohort was 66.5 years, and women accounted for 62% (n = 602) of the cohort (n = 971). Compared to men, women had worse left ventricular (LV) diastolic function and greater LV and arterial stiffness. After adjusting for covariates, the magnitude of the associations between age with septal E/e′ ratio, septal S′ velocity, effective arterial elastance (Ea) and LV end‐diastolic elastance (Eed) were greater in women. Sex differences in the magnitude of association between age with these four indices varied according to achieved SBP level. When achieved SBP <130 mm Hg, the magnitude of the associations between age with septal E/e′ ratio, septal S′ velocity, Ea and Eed did not differ by sex. Since age and sex are non‐modifiable, achieving SBP target, especially at a lower level, might be beneficial to attenuate sex differences in age‐related alterations in cardiac and arterial structure and function.