bluuebunny/crossref_metadata_2025_split huggingface dataset:
Detection of Human Papillomavirus Genotypes, Herpes Simplex, Varicella Zoster and Cytomegalovirus in Breast Cancer Patients
Authors: Mofrad Morvarid Golrokh , Sadigh Zohreh Azita , Ainechi Sanaz , Faghihloo Ebrahim 
Published: January 2021

Abstract
Background: The role of viruses as a cause of breast cancer (BC) has been significantly investigated in recent years. Human papillomavirus (HPV) has been detected in invasive breast carcinomas, while most studies have only focused on the detection of viral DNA, we aimed to examine the prevalence and genotypes of HPV among Iranian BC patients. We also examined the presence of herpes simplex-1 (HSV-1), herpes simplex-2 (HSV-2), varicella zoster virus (VZV), and cytomegalovirus (CMV) in these samples.Methods: We collected and analyzed 70 Formalin-Fixed Paraffin-Embedded (FFPE) blocks including 59 BC samples, and 11 benign breast lesions as control from Iranian patients using nested PCR. Real-time PCR utilized as a confirming test to nested PCR findings. Genotyping of HPV positive samples was performed, the samples were also subjected to a multiplex PCR to detect HSV-1, HSV-2, VZV, and CMV in BC.Results: Papillomavirus DNA was present in 7 of 59 BC samples (11.8%); while none was detected in control samples. The most prevalent type was HPV18, followed by HPV 6. All HPV positive patients had high tumor grades (II/ III) with a histologic diagnosis of ductal carcinoma. The patient age range was 33 to 73 years with a median of 51 years. Most of HPV positive patients had low levels of education. HPV16 was not detected. Also, 5 of 59 BC specimens (8.47%), were positive for HSV-1. But none of the samples were positive for HSV-2, VZV, and CMV.Conclusions: Our results suggest a carcinogenesis role for High-risk HPV (HPV18) in breast tumors. Our findings of HSV-1 and low-risk HPV (HPV6) in BCs may propose a cancer-causing role for them. Further large-scale studies are warranted to assess the significance of our findings.
WITHDRAWN: An integrated process of methanol coagulation and side-stream membrane bioreactor for the treatment of rice gruel wastewater
Authors: Nazia Shaik , Mishra Karishma , Jegatheesan Veeriah , Bhargava Suresh K , Sundergopal Sridhar 
Published: January 2021

Abstract
The demand for water supply is increasing rapidly across the world due to the exponential growth of population, swift urbanization, and industrialization. Methods for recovery of reusable water from domestic, industrial and agricultural wastewater should be cost-effective, and environmentally safe for the sustainability of water resources. Domestic wastewater is one of the contaminated water sources which need efficient treatment before discharge into water bodies. The novelty of the present study is to treat domestic rice gruel wastewater by coagulation integrated aerobic membrane bioreactor (AMBR) for the generation of reusable water. A hydrophilized ultrafiltration (HF-UF) spiral wound membrane of 5 kDa molecular weight cut off was used for the treatment of rice gruel wastewater at a feed temperature of 30 °C and 80 °C to study separation performance. Further, a comparison study was carried using various coagulants such as methanol, ethanol, and HCl for integration with HF-UF membrane to determine overall process efficiency at 30 °C. Methanol coagulation + HF-UF membrane based integrated process was able to achieve 93.77%, 95.4%, 91%, and 78.6 %, respectively of total dissolved solids (TDS), turbidity, conductivity, and chemical oxygen demand (COD). Further, this process was integrated with a bioreactor operated under aerobic conditions to improve the process efficiency and purified water quality. From the overall experimental results, the integrated coagulation + AMBR process attained a maximum removal of 93.9, 96, 91, and 94.6 percent of TDS, turbidity, conductivity and COD, correspondingly. Finally, an economic estimation of a 1000 Lh-1 capacity pilot plant is described in detail. The study depicts unique advantages of economy, process safety, small footprint, and eco-friendly for rice gruel wastewater by a combination of chemical coagulation with AMBR.
A Visual Framework of Meta Genomic Analysis on Variations of Whole SARS-CoV-2 Sequences
Authors: Zheng Jeffrey , Liu Jianzhong 
Published: January 2021

Abstract
The worldwide outbreak of the COVID-19 has become a global pandemic resulting in millions of confirmed cases and hundreds of thousands of deaths. To face such a global crisis, bioinformatics has played a key role in the diagnosis, follow-up, prognosis and treatment of COVID-19-infected patients.A novel bioinformatic tool for metagenomic analysis of whole genomes is proposed in this paper that is composed of three projections: global, clustering and genomic index. For each projection, key modules are described. Global projection provides various combinatorial distributions for a whole genome of N length, and the m-mer scheme partitions this sequence as M segments on 1D, 2D and 3D density matrices for multiple projections. Clustering projections based on distributions from global projections make special filters extract specific parts as probability eigenvalues. Genomic index projection provides comprehensive technologies under the theory of information entropy, and a list of measuring entropies are included, such as combinatorial entropy CE, integrated entropy IE, mean entropy ME and topological entropy TE. Three projections provide unified information to describe complicated functions, internal structures and refined variations for multiple groups of SARS-CoV-2 on variations and other genomes in comparisons.The outputs of three projections are illustrated on variant maps to support category, clustering, classification and establishing root activities for refined quantitative operations from bottom to top strategy.
Similarity Comparisons of SARS-CoV-2 Samples between Wuhan and G20
Authors: Wang Zhenhui , Qiao Mu , Zheng Jeffrey 
Published: January 2021

Abstract
To analyze SARS CoV-2 genomes, this paper uses a viral genome collected in Wuhan as a basic sequence to select a set of genomes from G20 countries/regions for comparison. Two methods are applied in visualization followed in the A1 and C1 modules of the MAS to provide 1D similarity projection and information entropy approaches. For a pair of two genomes segmented as M segments to calculate base differences corresponding to each segment, a measuring vector can be generated to be projected as a visual map. Refined information will be provided to calculate the information entropy corresponding to base differences. This provides quantitative measures to organize these genomes arranged into a linear order. The first method uses a line chart representation to illustrate relevant differences among genomes 1 by 1 between Wuhan and other regions. The second method uses a histogram representation to observe the information entropy projection results. Significant similarities are observed among the differences with approximately equal entropies with higher similarity. Based on the comparison of gene sequence diagrams between Wuhan and various regions, a difference analysis was carried out.
Observing Variations of Differences on COVID-19 in Varous Regions Extracting Type and Mutation Information
Authors: Liu Jianzhong , Zheng Jeffrey 
Published: January 2021

Abstract
Covid-19 genomes were collected from three regions: Shanghai-China, Tbilisi-Georgia and Sydney-Australia. Five similar genomes were selected from each region for research in this paper. Applying the “datum gene sequence” method proposed, our results show that variation is immense in the Sydney-Australia region, followed by variation in the Tbilisi-Georgia region, which has a minimal value in the Shanghai-China region.
Biological control of Meloidogyne spp. in glasshouse-grown chrysanthemum
Authors: De Long J. R. , Streminska M. A. , Persijn A. , Huisman H. M. I. , van der Salm C. 
Published: January 2021

Abstract
Root-knot nematodes (Meloidogyne spp.) are a major problem in soil-based glasshouse-grown chrysanthemums. To combat root-knot nematodes in the glasshouse, the soil is typically steamed every 5–6 production cycles. However, this method is expensive, environmentally unfriendly and reduces resistance and resilience of the soil against pathogens and pests. Here, we added biological pesticides/a basic substance and biostimulants both individually and in combination to determine individual or interactive effects against damage by root-knot nematodes in chrysanthemums. We found that the application of biological nematicides derived from garlic extract, the basic substance chitosan HCl and biostimulants comprised of sea minerals and plant oils correlated with reduced root-knot nematode damage. These effects may have been due to direct effects against the nematodes or through indirect effects such as increased resistance and resilience of the plants. Overall, the biostimulants increased the total number of free-living nematodes in the soil, which could lead to a beneficial increase in nutrient cycling in the soils. Our results demonstrate that biological reagents show promise in reducing root-knot nematode damage in glasshouse-grown chrysanthemum and may lead to more resistance and resilient soils.
Friction pendulum test and parameter optimization for the vibration reduction of a 100 kv isolating switch
Authors: Wang Hao , Wang Hongrun , Xu Yang 
Published: January 2021

An earthquake is a natural disaster with a high destructive force. According to previous history, a great many power grid facilities are damaged during the earthquake, and the damage to electrical equipment in substations are often very serious. This article takes the isolating switch in the substation as an experimental object. Firstly, the friction pendulum for vibration isolation is designed and manufactured according to the numerical simulation, and then the seismic test and vibration isolation test of the high voltage isolating switch are designed. The input waveforms and input acceleration intensities of the seismic test were designed experimentally. The test data of the isolating switch under the seismic condition and the vibration isolation condition with the friction pendulum vibration isolation system installed are mainly investigated. The measured acceleration of the top of the isolating switch bracket is used to compare and analyze the vibration isolation performance of the friction pendulum. Then, it can be seen that the friction pendulum system has the vibration isolation effects on the isolating switch. Especially, the isolation effects of friction pendulum are better when the acceleration intensity become higher. In order to obtain a better curvature radius and friction coefficient of the friction pendulum, based on the results of this test, the systematically numerical simulation tests for optimization on the friction pendulum vibration isolation system are conducted. The simulated top displacement of the isolating switch and the stress change of the root of the porcelain bottle were obtained. By comparing the vibration isolating performance of the friction pendulum under different parameters, numerical simulations show that the vibration isolation performance of the friction pendulum is better when the radius of curvature is 1200 mm and the friction coefficient is 0.04. The numerical results demonstrate that reasonable design of friction pendulum parameters can play a significant role in isolating vibration for power equipment.
Evaluating the Effectiveness of a Web-Based Program (POP4Teens) to Prevent Prescription Opioid Misuse Among Adolescents: Randomized Controlled Trial
Authors: Marsch Lisa A , Moore Sarah K , Grabinski Michael , Bessen Sarah Y , Borodovsky Jacob , Scherer Emily 
Published: January 2021

Background
Prescription opioid (PO) use is common among adolescents in the United States. Despite recent declines from unprecedented peaks in adolescent PO use (eg, in 2012-2013), there is seemingly paradoxical evidence that PO-related consequences (eg, opioid use disorder and overdoses) are increasing. These trends and their possible consequences emphasize the importance of prevention efforts targeting PO misuse. To our knowledge, we have developed the first interactive web-based program (POP4Teens [P4T]) focused specifically on the prevention of PO misuse among adolescents.

Objective
This study aimed to evaluate the effectiveness of P4T, a web-based program designed to prevent adolescent PO misuse, in comparison with JustThinkTwice (JTT), an active control website, on PO-related attitudes, knowledge, risk perception, and intentions to use.

Methods
We conducted a web-based randomized controlled trial in 2018. A total of 406 adolescents (aged 12-17 years) were randomly assigned to either P4T or JTT. The outcome variables were attitudes, knowledge, and risk perceptions associated with PO misuse, intentions to use POs, and program feedback. Data were collected at baseline and at 1, 3, and 6 months.

Results
Both programs resulted in significant and sustained improvements in intention to use POs, increased perceived risk, impacted expectancies consistent with prevention, and improved PO refusal skills. P4T produced significantly greater increases in PO-related knowledge than JTT did, and it was reportedly easier to use and more liked. Baseline scores for youth reporting past-year medical use of POs, friends who engage in nonmedical use of POs, and/or poor mental health underscored their at-risk status compared with youth from the other groups.

Conclusions
P4T positively impacted all study variables that are known to prevent PO misuse among teens. Moreover, its web-based nature simplifies the dissemination and implementation of this novel tool designed to help meet the challenges of the evolving national opioid crisis.

Trial Registration
ClinicalTrials.gov NCT02737696; https://clinicaltrials.gov/ct2/show/NCT02737696
Efficacy of Smart Speaker–Based Metamemory Training in Older Adults: Case-Control Cohort Study
Authors: Kim Jeongsim , Shin EunJi , Han KyungHwa , Park Soowon , Youn Jung Hae , Jin Guixiang , Lee Jun-Young 
Published: January 2021

BackgroundMetamemory training (MMT) is a useful training strategy for improving cognitive functioning in the older adult population. Despite the advantages, there are limitations imposed by location and time constraints.ObjectiveThis study aimed to develop a smart speaker–based MMT program and evaluate the efficacy of the program in older adults without cognitive impairment.MethodsThis study used a case-control cohort design. The smart speaker–based MMT program comprised 3 training sessions per day, 5 days a week, for 8 weeks. Each training session took approximately 15 minutes. This program was implemented using smart speakers, not human trainers. All participants completed the Mini-Mental State Examination, Subjective Memory Complaints Questionnaire, Verbal Learning Test, Digit Span Test, fluency tests, and a short-form version of the Geriatric Depression Scale before and after training.ResultsA total of 60 subjects (29 in the MMT group and 31 in the control group) participated in the study. The training group showed significant increases in the delayed free recall, digit span forward, digit span backward, and fluency test scores compared with the control group.ConclusionsThis study confirmed the efficacy of smart speaker–based MMT in older adults. Home-based smart speaker–based MMT is not limited with respect to location or constrained by space and may help older adults with subjective cognitive decline without requiring intervention by human professionals.
Conducting Virtual, Synchronous Focus Groups Among Black Sexual Minority Men: Qualitative Study
Authors: Dangerfield II Derek T , Wylie Charleen , Anderson Janeane N 
Published: January 2021

Background
Focus groups are useful to support HIV prevention research among US subpopulations, such as Black gay, Black bisexual, and other Black sexual minority men (BSMM). Virtual synchronous focus groups provide an electronic means to obtain qualitative data and are convenient to implement; however, the protocols and acceptability for conducting virtual synchronous focus groups in HIV prevention research among BSMM are lacking.

Objective
This paper describes the protocols and acceptability of conducting virtual synchronous focus groups in HIV prevention research among BSMM

Methods
Data for this study came from 8 virtual synchronous focus groups examined in 2 studies of HIV-negative BSMM in US cities, stratified by age (N=39): 2 groups of BSMM ages 18-24 years, 5 groups of BSMM ages 25-34 years, and 1 group of BSMM 35 years and older. Virtual synchronous focus groups were conducted via Zoom, and participants were asked to complete an electronic satisfaction survey distributed to their email via Qualtrics.

Results
The age of participants ranged from 18 to 44 years (mean 28.3, SD 6.0). All participants “strongly agreed” or “agreed” that they were satisfied participating in an online focus group. Only 17% (5/30) preferred providing written informed consent versus oral consent. Regarding privacy, most (30/30,100%) reported “strongly agree” or “agree” that their information was safe to share with other participants in the group. Additionally, 97% (29/30) reported being satisfied with the incentive.

Conclusions
Conducting virtual synchronous focus groups in HIV prevention research among BSMM is feasible. However, thorough oral informed consent with multiple opportunities for questions, culturally relevant facilitation procedures, and appropriate incentives are needed for optimal focus group participation.
Early Perceptions of COVID-19 Contact Tracing Apps in German-Speaking Countries: Comparative Mixed Methods Study
Authors: Zimmermann Bettina Maria , Fiske Amelia , Prainsack Barbara , Hangel Nora , McLennan Stuart , Buyx Alena 
Published: January 2021

Background
The main German-speaking countries (Germany, Austria, and Switzerland) have implemented digital contact tracing apps to assist the authorities with COVID-19 containment strategies. Low user rates for these apps can affect contact tracing and, thus, its usefulness in controlling the spread of the novel coronavirus.

Objective
This study aimed to assess the early perceptions of people living in the German-speaking countries and compare them with the frames portrayed in the newspapers during the first wave of the COVID-19 pandemic.

Methods
We conducted qualitative interviews with 159 participants of the SolPan project. Of those, 110 participants discussed contact tracing apps and were included in this study. We analyzed articles regarding contact tracing apps from 12 newspapers in the German-speaking countries.

Results
Study participants perceived and newspaper coverage in all German-speaking countries framed contact tracing apps as governmental surveillance tools and embedded them in a broader context of technological surveillance. Participants identified trust in authorities, respect of individual privacy, voluntariness, and temporary use of contact tracing apps as prerequisites for democratic compatibility. Newspapers commonly referenced the use of such apps in Asian countries, emphasizing the differences in privacy regulation among these countries.

Conclusions
The uptake of digital contact tracing apps in German-speaking countries may be undermined due to privacy risks that are not compensated by potential benefits and are rooted in a deeper skepticism towards digital tools. When authorities plan to implement new digital tools and practices in the future, they should be very transparent and proactive in communicating their objectives and the role of the technology—and how it differs from other, possibly similar, tools. It is also important to publicly address ethical, legal, and social issues related to such technologies prior to their launch.
Expanding Telemonitoring in a Virtual World: A Case Study of the Expansion of a Heart Failure Telemonitoring Program During the COVID-19 Pandemic
Authors: Wali Sahr , Guessi Margarido Milena , Shah Amika , Ware Patrick , McDonald Michael , O'Sullivan Mary , Duero Posada Juan , Ross Heather , Seto Emily 
Published: January 2021

Background
To minimize the spread and risk of a COVID-19 outbreak, societal norms have been challenged with respect to how essential services are delivered. With pressures to reduce the number of in-person ambulatory visits, innovative models of telemonitoring have been used during the pandemic as a necessary alternative to support access to care for patients with chronic conditions. The pandemic has led health care organizations to consider the adoption of telemonitoring interventions for the first time, while others have seen existing programs rapidly expand.

Objective
At the Toronto General Hospital in Ontario, Canada, the rapid expansion of a telemonitoring program began on March 9, 2020, in response to COVID-19. The objective of this study was to understand the experiences related to the expanded role of a telemonitoring program under the changing conditions of the pandemic.

Methods
A single-case qualitative study was conducted with 3 embedded units of analysis. Semistructured interviews probed the experiences of patients, clinicians, and program staff from the Medly telemonitoring program at a heart function clinic in Toronto, Canada. Data were analyzed using inductive thematic analysis as well as Eakin and Gladstone’s value-adding approach to enhance the analytic interpretation of the study findings.

Results
A total of 29 participants were interviewed, including patients (n=16), clinicians (n=9), and operational staff (n=4). Four themes were identified: (1) providing care continuity through telemonitoring; (2) adapting telemonitoring operations for a more virtual health care system; (3) confronting virtual workflow challenges; and (4) fostering a meaningful patient-provider relationship. Beyond supporting virtual visits, the program’s ability to provide a more comprehensive picture of the patient’s health was valued. However, issues relating to the lack of system integration and alert-driven interactions jeopardized the perceived sustainability of the program.

Conclusions
With the reduction of in-person visits during the pandemic, virtual services such as telemonitoring have demonstrated significant value. Based on our study findings, we offer recommendations to proactively adapt and scale telemonitoring programs under the changing conditions of an increasingly virtual health care system. These include revisiting the scope and expectations of telemedicine interventions, streamlining virtual patient onboarding processes, and personalizing the collection of patient information to build a stronger virtual relationship and a more holistic assessment of patient well-being.
Aesthetic Principles
Authors: Sarhaddi Deniz , Nahai Foad 
Published: January 2021

The perception of beauty impacts our daily lives, such that the pursuit of beauty is often the pursuit of an improved quality of life. Although there are guidelines for facial proportions, there is no set beauty ideal in the modern day. Aesthetic surgeons should be familiar with facial proportions and should be able to modify these with respect to cultural and ethnic variations. To achieve imperceptible scarring, aesthetic surgeons should be able to control tissue tension, properly orient incisions, appreciate anatomical subunits, and avoid distortion of surrounding structures. Aesthetic surgery, when performed with craftsmanship, can achieve a long-lasting and harmonious result.
This review contains 10 figures, and 26 references.
Keywords: defining beauty, facial aesthetic proportions, facial subunits, scarring, resting skin tension lines, evolution of facelift, SMAS, facial aging