bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Morphology of Inner Cell Mass Is the Strongest Predictor of Live Birth After a Frozen-Thawed Single Embryo Transfer
Authors: Ai Jihui , Jin Lei , Zheng Yu , Yang Peiwen , Huang Bo , Dong Xiyuan 
Published: February 2021

BackgroundThe scoring system for human blastocysts is traditionally based on morphology; however, there are controversies on the effect of morphology parameters on pregnancy outcomes. The aim of this study is to evaluate the predicting value of each morphology parameter on pregnancy outcomes in a setting of single embryo transfer.MethodsThis is a retrospective cohort study on patients undergoing frozen-thawed single blastocyst transfer at our center, between Jan. 2009 and Dec. 2018. A total of 10,482 cycles were analyzed. The blastocysts were scored according to the expansion and hatching status, morphology of inner cell mass (ICM), and cells of trophectoderm (TE). The primary outcome measure was live birth rate. One-way analysis of variance, chi-square test, and multiple logistic regression were used for statistical analysis.ResultsThe clinical pregnancy rate was lower in the blastocysts of stage 3 (48.15%), compared with those of stage 4 (56.15%), stage 5 (54.91%), and stage 6 (53.37%). The live birth rate was lower in the blastocysts of stage 3 (37.07%), compared with those of stage 4 (44.21%) and stage 5 (41.67%). The rates of clinical pregnancy (A: 66.60%, B: 53.25%, C: 39.33%) and live birth (A: 54.62%, B: 41.29%, C: 28.45%) were both decreased with decreasing grade of ICM morphology, and these differences were pairwise significant. The miscarriage rate of blastocysts with ICM grade A was lower, compared with ICM grade C (17.53 vs. 27.66%). Blastocysts with TE morphology of C had lower rates of clinical pregnancy (43.53%) and live birth (32.57%), compared with those with TE morphology of A and B (clinical pregnancy rate: 64.26% for A, 58.11% for B; live birth rate: 52.74% for A, 45.64% for B). There were no significant differences in rates of clinical pregnancy, live birth, and miscarriage between the blastocysts with TE grade A and B.ConclusionsThe blastocyst expansion stage, ICM grade, and TE grade are all associated with pregnancy outcomes. ICM grade is the strongest predictor of live birth. A blastocyst with stage 4–5, ICM grade A, and TE grade A/B should be given priority for single embryo transfer.
Longitudinal Analysis of Anti-cardiolipin and Anti-β2-glycoprotein-I Antibodies in Recent-Onset Systemic Lupus Erythematosus: A Prospective Study in Swedish Patients
Authors: Frodlund Martina , Walhelm Tomas , Dahle Charlotte , Sjöwall Christopher 
Published: February 2021

Background: Anti-phospholipid syndrome (APS) and systemic lupus erythematous (SLE) are autoimmune disorders that often co-occur. Anti-phospholipid antibodies (aPL) are typical of both conditions and may be associated with vascular events and pregnancy-related morbidities. Whereas, aPL-screening is mandatory for individuals with suspected SLE, the clinical value of longitudinal aPL analyses in established SLE is unclear.Methods: We investigated the occurrence and variation of IgG/IgA/IgM anti-cardiolipin (aCL) and anti-β2-glycoprotein-I (anti-β2GPI) antibodies, using both the manufacturer's cut-off and a cut-off based on the 99th percentile of 400 apparently healthy donors, in recent-onset SLE. Furthermore, we evaluated the relationships between aPL levels and SLE/APS manifestations, as well as the pharmacotherapy. Patients with SLE who met validated classification criteria were included in this prospective study (N = 54). Samples were obtained at 0, 6, 12, 24, 36, 48, 60, 72, 84, and 96 months after SLE diagnosis.Results: Depending on the cut-off applied, 61.1 or 44.4% showed a positive result for at least one aPL isotype or the lupus anticoagulant test over time. Median values for all six aPL isotypes numerically decreased from inclusion to last follow-up, but none of the isotypes met statistical significance. Seroconversion (from positive to negative, or the opposite direction) was occasionally seen for both aCL and anti-β2GPI. IgA and IgM anti-β2GPI were the most common isotypes, followed by IgM aCL. Presence of IgG aCL associated significantly with myocardial infarction and miscarriage, and IgG/IgA anti-β2GPI with miscarriage.Conclusion: aPL were common during the first years of SLE. Even though the levels fluctuated over time, the patients tended to remain aPL positive or negative. Repeated aPL testing in the absence of new symptoms seems to be of uncertain value in patients with recent-onset SLE.
Imaging of Vulva Syringoma With Reflectance Confocal Microscopy
Authors: Feng Lin , Lin Yan , Wang Leilei , Chen Hongxiao , Gao Min , Liu Huaxu , Yang Hongyu 
Published: February 2021

Objectives: To investigate the application of reflectance confocal microscopy (RCM) imaging in diagnosis of vulva syringoma.Methods: Patients with lesions suspicious of syringoma on vulva were enrolled in the study. After informed consent was taken, the lesions were photographed and imaged with RCM. The features of the lesion in confocal images were then analyzed and compared with the biopsy findings for histology correlation.Results: Eleven cases in total were included in the study. The typical RCM features observed in syringoma are the presence of round to oval high refractive, and relatively monomorphous mass of varying sizes in the superficial and middle dermis, usually surrounded with 1–2 layers of light-dark line structures, which were further confirmed by histological evaluation. Ten cases showed classic features of syringoma and 1 case exhibited milia in RCM images.Conclusions: Syringoma has distinct features in RCM imaging, which correlates well with histological findings, highlighting the potential role of RCM in the diagnosis and differential diagnosis of vulva syringoma.
Resilience to Plasma and Cerebrospinal Fluid Amyloid-β in Cognitively Normal Individuals: Findings From Two Cohort Studies
Authors: Lin Li , Sun Yu , Wang Xiaoqi , Su Li , Wang Xiaoni , Han Ying 
Published: February 2021

Objective: To define resilience metrics for cognitive decline based on plasma and cerebrospinal fluid (CSF) amyloid-β (Aβ) and examine the demographic, genetic, and neuroimaging factors associated with interindividual differences among metrics of resilience and to demonstrate the ability of such metrics to predict the diagnostic conversion to mild cognitive impairment (MCI).Methods: In this study, cognitively normal (CN) participants with Aβ-positive were included from the Sino Longitudinal Study on Cognitive Decline (SILCODE, n = 100) and Alzheimer's Disease Neuroimaging Initiative (ADNI, n = 144). Using a latent variable model of data, metrics of resilience [brain resilience (BR), cognitive resilience (CR), and global resilience (GR)] were defined based on the plasma Aβ and CSF Aβ. Linear regression analyses were applied to investigate the association between characteristics of individuals (age, sex, educational level, genetic, and neuroimaging factors) and their resilience. The plausibility of these metrics was tested using linear mixed-effects models and Cox regression models in longitudinal analyses. We also compared the effectiveness of these metrics with conventional metrics in predicting the clinical progression.Results: Although individuals in the ADNI cohort were older (74.68 [5.65] vs. 65.38 [4.66], p &lt; 0.001) and had higher educational levels (16.3 [2.6] vs. 12.6 [2.8], p &lt; 0.001) than those in the SILCODE cohort, similar loadings between resilience and its indicators were found within both models. BR and GR were mainly associated with age, women, and brain volume in both cohorts. Prediction models showed that higher CR and GR were related to better cognitive performance, and specifically, all types of resilience to CSF Aβ could predict longitudinal cognitive decline.Conclusion: Different phenotypes of resilience depending on cognition and brain volumes were associated with different factors. Such comprehensive resilience provided insight into the mechanisms of susceptibility for Alzheimer's disease (AD) at the individual level, and interindividual differences in resilience had the potential to predict the disease progression in CN people.
Bone-Derived Factors as Potential Biomarkers for Parkinson’s Disease
Authors: Lin Yuwan , Zhou Miaomiao , Dai Wei , Guo Wenyuan , Qiu Jiewen , Zhang Zhiling , Mo Mingshu , Ding Liuyan , Ye Panghai , Wu Yijuan , Zhu Xiaoqin , Wu Zhuohua , Xu Pingyi , Chen Xiang 
Published: February 2021

Background: Parkinson’s disease (PD) and osteoporosis are both common aging diseases. It is reported that PD has a close relationship with osteoporosis and bone secretory proteins may be involved in disease progression.Objectives: To detect the bone-derived factors in plasma and cerebrospinal fluid (CSF) of patients with PD and evaluate their correlations with C-reaction protein (CRP) level, motor impairment, and Hoehn-Yahr (HY) stage of the disease.Methods: We included 250 PD patients and 250 controls. Levels of osteocalcin (OCN), osteopontin (OPN), osteoprotegerin (OPG), Sclerostin (SO), Bone morphogenetic protein 2 (BMP2), and Dickkopf-1 (DKK-1) in plasma and CSF were measured by custom protein antibody arrays. Data were analyzed using Mann–Whitney U-test and Spearman’s receptor activator of NF-κB (RANK) correlation.Results: Plasma levels of OCN and OPN were correlated with CRP levels and HY stage and motor impairment of PD. Furthermore, the plasma assessment with CSF detection may enhance their potential prediction on PD.Conclusions: OCN and OPN may serve as potential biomarkers for PD. The inflammation response may be involved in the cross-talk between the two factors and PD.
Advanced NSCLC Patients With EGFR T790M Harboring TP53 R273C or KRAS G12V Cannot Benefit From Osimertinib Based on a Clinical Multicentre Study by Tissue and Liquid Biopsy
Authors: Fu Yulong , Wang Anqi , Zhou Jieqi , Feng Wei , Shi Minhua , Xu Xiao , Zhao Hongqing , Cai Liming , Feng Jian , Lv Xuedong , Zhang Xiaodong , Xu Wenjing , Zhang Zhengrong , Ma Guoer , Wang Jian , Zhou Tong , Zhao Dahai , Fang Haohui , Liu Zeyi , Huang Jian-an 
Published: February 2021

BackgroundNon-small cell lung cancer (NSCLC) patients treated with first-generation epidermal growth factor receptor-tyrosine kinase inhibitor (EGFR-TKI) almost always acquire resistance, and the development of novel techniques analyzing circulating tumor DNA (ctDNA) have made it possible for liquid biopsy to detect genetic alterations from limited amount of DNA with less invasiveness. While a large amount of patients with EGFR exon 21 p.Thr790 Met (T790M) benefited from osimertinib treatment, acquired resistance to osimertinb has subsequently become a growing challenge.MethodsWe performed tissue and liquid rebiopsy on 50 patients with EGFR-mutant NSCLC who acquired resistance to first-generation EGFR-TKIs. Plasma samples underwent droplet digital PCR (ddPCR) and next-generation sequencing (NGS) examinations. Corresponding tissue samples underwent NGS and Cobas® EGFR Mutation Test v2 (Cobas) examinations.ResultsOf the 50 patients evaluated, the mutation detection rates of liquid biopsy group and tissue biopsy group demonstrated no significant differences (41/48, 85.4% vs. 44/48, 91.7%; OR=0.53, 95% CI=0.15 to 1.95). Overall concordance, defined as the proportion of patients for whom at least one identical genomic alteration was identified in both tissue and plasma, was 78.3% (36/46, 95% CI=0.39 to 2.69). Moreover, our results showed that almost half of the patients (46%, 23/50) resistant to first-generation EGFR-TKI harbored p.Thr790 Met (T790M) mutation. 82.6% (19/23) of the T790M positive patients were analyzed by liquid biopsy and 60.9% (14/23) by tumor tissue sequencing. Meanwhile, a wide range of uncommon mutations was detected, and novel mechanisms of osimertinib resistance were discovered. In addition, 16.7% (2/12) of the T790M positive patients with either TP53 R237C or KRAS G12V failed to benefit from the subsequent osimertinib treatment.ConclusionOur results emphasized that liquid biopsy is applicable to analyze the drug resistance mechanisms of NSCLC patients treated with EGFR-TKIs. Moreover, we discovered two uncommon mutations, TP53 R273C and KRAS G12V, which attenuates the effectiveness of osimertinib.
Effects of High-Intensity Interval Training and Moderate-Intensity Training on Stress, Depression, Anxiety, and Resilience in Healthy Adults During Coronavirus Disease 2019 Confinement: A Randomized Controlled Trial
Authors: Borrega-Mouquinho Yolanda , Sánchez-Gómez Jesús , Fuentes-García Juan Pedro , Collado-Mateo Daniel , Villafaina Santos 
Published: February 2021

Objective: This study aimed to compare the effects of two intervention programs, (1) high-intensity interval training (HIIT) and (2) moderate-intensity training (MIT), on anxiety, depression, stress, and resilience during the confinement caused by the coronavirus disease 2019 (COVID-19) in healthy adults.Methods: A total of 67 adults who participated were randomly assigned to two groups: HIIT and MIT groups. The MIT group had to perform a home-based intervention based on aerobic exercises, whereas the HIIT group had to perform a home-based intervention based on HIIT exercises. The two groups (HIIT and MIT) had to complete the same physical exercise volume, 40 min per session (6 days per week) during the confinement period (6 weeks). Depression, anxiety, stress, and resilience were assessed before and after the intervention.Results: Results showed that HIIT and MIT significantly reduced the stress, anxiety, and depression as well as increase the resilience (p &lt; 0.05). Moreover, the improvements obtained in the HIIT group seem to be greater than those of the MIT group in depression (p &lt; 0.05).Conclusions: HIIT and MIT decreased anxiety, stress, and depression as well as increased resilience during the COVID-19 confinement. In addition, the HIIT intervention seemed to be more beneficial to reduce depression than the MIT intervention.
MENINGKATKAN NILAI PERUSAHAAN MELALUI GREEN INNOVATION DAN ECO-EFFISIENSI
Authors: Dewi Rosiyana , Rahmianingsih Ajeng 
Published: February 2021

Kondisi bumi dan lingkungan yang semakin memburuk saat ini menjadi salah satu dasar penilaian utama para pemangku kepentingan dalam mengambil keputusan. Sebuah perusahaan diharapkan memiliki peran dan partisipasi terhadap perbaikan lingkungan. Tujuan penelitian ini adalah untuk mengetahui pengaruh green innovation dan eco-efficiency terhadap nilai perusahaan pada perusahaan yang terdaftar di Bursa Efek Indonesia. Data penelitian didapat dari laporan tahunan, laporan keberlanjutan dari website perusahaan atau situs Bursa Efek Indonesia. Sampel yang digunakan adalah 45 perusahaan yang memenuhi kriteria dan terdaftar di Bursa Efek Indonesia (BEI) pada tahun 2016-2018. Teknik pengambilan sampel yang digunakan adalah purposive sampling. Pengujian hipotesis pada penelitian ini menggunakan metode analisis regresi linear berganda. Hasil penelitian ini menunjukan bahwa Green innovation berpengaruh positif terhadap Eco-efficiency, Eco efficiency berpengaruh positif terhadap nilai perusahaan, Green Innovation berpengaruh positif terhadap Nilai perusahaan dan Eco-efficiency tidak memediasi pengaruh Green Innovation terhadap Nilai perusahaan.
The case study of Villa Beatrice d’Este: management planning of a multi-layered site with Medieval ruins in the Veneto Region, Italy
Authors: Gamba Valentina , Calò Sergio , Malé Maurizio , Moretto Enzo 
Published: February 2021

Villa Beatrice d’Este is a 17th century Venetian Villa in the Veneto Region, Italy, located within the area of the Euganean Hills Regional Park. The villa was built to replace a previous 13th century Medieval Monastery, whose structure was integrated in the villa. Remains of the Medieval structures are still visible today. The monumental complex constitutes an example of a multi-layered site with continuous life from Medieval times up to the present days. For this reason, the site was selected as a case study by the EU-funded project RUINS[1], in view of proposing a management plan to protect and valorise its complex heritage, as an example of heritage site with Medieval ruins in Europe.

[1] RUINS, Sustainable re-use, preservation and modern management of historical ruins in Central Europe - elaboration of integrated model and guidelines based on the synthesis of the best European experiences. A project funded by the EU through the Interreg Central Europe Programme.
Sustainable re-use, preservation and modern management of historical ruins. RUINS’ tools & guidelines
Authors: Soldano Silvia 
Published: February 2021

Owners and managers of the thousands of medieval ruins around Europe face the challenge of preservation despite limited options for modern use of their sites. The RUINS project develops approaches that help managers to find contemporary, social uses for old ruins while keeping historical heritage intact. Research and evaluation of various sites will create a basis for comprehensive management plans.
A lack of functionality of medieval ruins leaves limited opportunities for establishing a viable economic future of these sites. Giving new functions to ruins can result in broad, economically profitable ways of using the medieval ruins.
The aim is finding the balance between the needs of stakeholders and public expectations concerning use of medieval ruins on one hand, and on the other hand preservation of authenticity and historical value of medieval ruins.
The individuation of the new function requires a specific knowledge of the building in all its aspects, but also considerations regarding socio-economic values of the context that identifies its historical meaning and artistic value. The approach is to find new aims to monuments, but in harmony with the characteristics that give value and meaning to them.
Within the INTERREG Central Europe project RUINS, is developing an handbook that supplies an operational tool useful to guide owners and managers of the thousands of medieval ruins around Europe toward a stainable re-use, preservation and modern management of historical ruins.
The work follows an outline composed by 5 chapters that will try to respond in an exhaustive way to the aims related to contemporary use of historical ruins.
ZMIANY W KRAJOBRAZIE KULTUROWYM WYWOŁANE REKONSTRUKCJĄ GRODZISKA ŚREDNIOWIECZNEGO W OWIDZU (GMINA STAROGARD GD.)
Authors: Łaguna Wanda , Rayss Joanna 
Published: February 2021

W referacie autorzy przedstawiają wyniki działań projektowych i inwestycyjnych związanych z „Rekonstrukcją wczesnośredniowiecznego grodziska w Owidzu, w gminie Starogard Gdański, wraz z infrastrukturą towarzyszącą”. Grodzisko w Owidzu jest jednym z większych rozpoznanych grodzisk wczesnośredniowiecznych w Polsce Północnej. Zajmuje obszar ponad 2 ha i posiada własną formę krajobrazową. Zlokalizowane jest w zakolu rzeki Wierzyca, w odległości 2 km od Starogardu Gdańskiego, w pobliżu miejscowości pofolwarcznej Owidz. Opisywany projekt uzyskał dofinansowanie ze środków unijnych w ramach programu Infrastruktura i Środowisko i realizowany jest w 4 etapach. Zakończony został już I etap inwestycji, który dotyczył rekonstrukcji samego grodziska oraz budowy budynku wystawienniczo-dydaktycznego. Etap II, kończący się we wrześniu, dotyczy budowy amfiteatru i budynku wieży schodowej wraz z sanitariatami, natomiast etapy III i IV znajdują się w trakcie przygotowania inwestycji. Całe założenie, oprócz rekonstrukcji grodziska średniowiecznego jako ekspozycji, ma spełniać również rolę dydaktyczną, kulturową i rekreacyjną. Do badań na potrzeby niniejszego artykułu wykorzystano bogaty materiał zdjęciowy uzyskany podczas procesu projektowego oraz w trakcie nadzoru autorskiego przy realizacji I i II etapu.
PENGARUH MOTIVASI DAN GAYA KEPEMIMPINAN TERHADAP KINERJA PEGAWAI PADA KANTOR RUMAH TAHANAN NEGARA KELAS IIB SELAYAR
Authors: Hasnawati Hasnawati , Sapiri Muchtar , Ruslan Muhlis 
Published: February 2021

Penelitian ini bertujuan buat mengetahui korelasi antara dampak motivasi serta gaya kepemimpinan terhadap kinerja pegawai pada rumah Tahanan Negara Kelas II B Selayar. Metode analisis yg dipergunakan merupakan analisis regresi linear berganda menggunakan contoh uji-t serta uji-f. Dari akibat pengujian regresi linear berganda diketahui bahwa motivasi serta gaya kepemimpinan secara Partial dan simultaneous berpengaruh positif serta significan terhadap hasil kerja pegawai. Hal ini sebagai bukti empiric bahwa indikator-indikator yg dipakai dalam kegiatan penelitian mengenai usaha kerja digunakan sebagai dasar buat peningkatan kinerja pegawai di pada tempat tinggal tahanan kelas II B Selayar. berasal yang akan terjadi uji regresi dapat dicermati bahwa variabel motivasi serta gaya kepemimpinan yang lebih bertenaga mempengaruhi peningkatan kinerja pegawai pada tempat tinggal tahanan kelas II B Selayar.
This study aims to determine the correlation between the impact of motivation and leadership style on employees’ performance in the Detention Center Class II B Selayar. The analytical method used is multiple linear regression analysis using the sample t-test and f-test. From the results of multiple linear regression testing, it is known that motivation and leadership style, partially and simultaneously, have positive and significant impacts on employees’ work results. This is empirical evidence that the indicators used in this research activities regarding work effort are used as a basis for improving employees’ performance in the Detention Center Class II B Selayar. From the regression test, it can be seen that motivation and leadership style variables significantly influence the improvement of employees’ performance in the Detention Center Class II B Selayar.
Efficacy of Tocilizumab in the treatment of severe COVID-19 patients with respiratory failure
Authors: Adıyeke Esra , Coşkun Nilüfer , Bakan Nurten , Demir Serkan , Cihan Murat , Yiyit Nurettin 
Published: February 2021

Objective: This study aimed to investigate the impact of tocilizumab, an IL6R inhibitor, on clinical features and laboratory tests of subjects admitted with severe COVID-19 and respiratory failure.
Material and Methods: A total of 30 patients with positive polymerase chain reaction for COVID-19 and respiratory failure were analyzed in a retrospective manner. All patients received 8mg/kg body weight tocilizumab i.v. once in addition to the standard COVID-19 treatment protocol, including Oseltamivir phosphate 75mg twice daily, hydroxychloroquine 200 mg twice daily, and azitromycine 250 mg once daily following a 500 mg loading dose. Demographic characteristics, and clinical features including oxygen saturation, the concentration of oxygen inhalation, body temperature, mean arterial pressure and heart rate, and SpO2, end-tidal CO2, and blood tests including complete blood count, procalcitonin, C-reactive protein (CRP), Troponin-I, D-dimer, and liver and kidney function tests were recorded before and after treatment with tocilizumab.
Results: A significant increase occurred in SaO2 on first and third days following treatment with tocilizumab (84.3 % and 90.3%, respectively, p<0.001 for both recordings compared to baseline). There was also a significant increase in end-tidal CO2. The increase in mean SaO2 after tocilizumab was followed by a decline in respiratory rate on the first and third days of treatment. A dramatic decline was observed in body temperature from the first day of treatment with tocilizumab. Lymphocyte count increased following tocilizumab and C-reactive protein and Troponin I levels were reduced.
Conclusion: Tocilizumab appears as an effective therapeutic option for improving oxygenation, symptoms and laboratory surrogates of ongoing inflammation in subjects with severe COVID-19.