bluuebunny/crossref_metadata_2025_split huggingface dataset:
Bacterial Community Analysis and Potential Functions of Core Taxa in Different Parts of the Fungus Cantharellus cibarius
Authors: GE WEI , ZHANG ZHI-YUAN , DONG CHUN-BO , HAN YAN-FENG , DESHMUKH SUNIL K. , LIANG ZONG-QI 
Published: September 2021

Abstract

Cantharellus cibarius is a widely distributed, popular, edible fungus with high nutritional and economic value. However, significant challenges persist in the microbial ecology and artificial cultivation of C. cibarius. Based on the 16S rRNA sequencing data, this study analyzed bacterial community structures and diversity of fruit bodies and rhizomorph parts of C. cibarius and mycosphere samples (collected in the Wudang District, Guiyang, Guizhou Province, China). It explored the composition and function of the core bacterial taxa. The analyzed results showed that the rhizomorph bacterial community structure was similar to mycosphere, but differed from the fruit bodies. Members of the Allorhizobium-Neorhizobium-Pararhizobium-Rhizobium complex had the highest abundance in the fruit bodies. However, they were either absent or low in abundance in the rhizomorphs and mycosphere. At the same time, members of the Burkholderia-Caballeronia-Paraburkholderia complex were abundant in the fruit bodies and rhizomorphs parts of C. cibarius, as well as mycosphere. Through functional annotation of core bacterial taxa, we found that there was an apparent trend of potential functional differentiation of related bacterial communities in the fruit body and rhizomorph: potential functional groups of core bacterial taxa in the fruit bodies centered on nitrogen fixation, nitrogen metabolism, and degradation of aromatic compounds, while those in rhizomorphs focused on aerobic chemoheterotrophy, chemoheterotrophy, defense against soil pathogens, decomposition of complex organic compounds, and uptake of insoluble inorganic compounds. The analysis of functional groups of bacteria with different structures is of great significance to understand that bacteria promote the growth and development of C. cibarius.
Rapid Detection and Differentiation of KPC and MBL Carbapenemases among Enterobacterales Isolates by a Modified Combined-Disk Test
Authors: WEI MING , WANG PENG , WANG SHUAI , YANG CHUNXIA , GU LI 
Published: September 2021

Abstract
This study was conducted to develop a cheap, rapid, and accurate modified combined-disk test (mCDT) approach to detect and differentiate KPC and MBL carbapenemases among clinical carbapenem-resistant Enterobacterales (CRE) isolates and simultaneously distinguish them from carbapenem-susceptible Enterobacterales (CSE) isolates. A total of 163 CRE and 90 third-generation cephalosporin-resistant Enterobacterales isolates were tested using imipenem and meropenem disks and different concentrations of carbapenemase inhibitors. The optimal sensitivity and specificity for detecting KPC carbapenemase were 97.2% and 100%, respectively. The sensitivity and specificity for detecting MBL carbapenemase were 100% and 100% with imipenem or meropenem and carbapenemase inhibitors within six hours. The inhibitory zone diameter of 18 mm for imipenem or meropenem disks without inhibitor could distinguish CRE from CSE isolates. Therefore, this mCDT approach may be a useful tool in clinical laboratories to detect CRE isolates and differentiate KPC and MBL producers, which is beneficial for patient management and hospital infection prevention and control.
Comparative Genomic Analysis and Phenotypic Characterization of Bronchoscope-Associated Klebsiella aerogenes
Authors: HUANG FANG , LI SHUANG , LOU LAN , MO JUNJUN , XU HAO 
Published: September 2021

Abstract
Bronchoscopes have been linked to outbreaks of nosocomial infections. The phenotypic and genomic profiles of bronchoscope-associated Klebsiella aerogenes isolates are largely unknown. In this work, a total of 358 isolates and 13 isolates were recovered from samples after clinical procedures and samples after decontamination procedures, respectively, over the five months. Antimicrobial susceptibility testing found seven K. aerogenes isolates exhibiting a low-level resistance to antimicrobial agents. Among seven K. aerogenes isolates, we found five sequence types (STs) clustered into three main clades. Collectively, this study described for the first time the phenotypic and genomic characteristics of bronchoscope-associated K. aerogenes.
Fungal Infections in COVID-19 Intensive Care Patients
Authors: COŞKUN AYŞENUR SÜMER , DURMAZ ŞENAY ÖZTÜRK 
Published: September 2021

Abstract
Opportunistic fungal infections increase morbidity and mortality in COVID-19 patients monitored in intensive care units (ICU). As patients’ hospitalization days in the ICU and intubation period increase, opportunistic infections also increase, which prolongs hospital stay days and elevates costs. The study aimed to describe the profile of fungal infections and identify the risk factors associated with mortality in COVID-19 intensive care patients. The records of 627 patients hospitalized in ICU with the diagnosis of COVID-19 were investigated from electronic health records and hospitalization files. The demographic characteristics (age, gender), the number of ICU hospitalization days and mortality rates, APACHE II scores, accompanying diseases, antibiotic-steroid treatments taken during hospitalization, and microbiological results (blood, urine, tracheal aspirate samples) of the patients were recorded. Opportunistic fungal infection was detected in 32 patients (5.10%) of 627 patients monitored in ICU with a COVID-19 diagnosis. The average APACHE II score of the patients was 28 ± 6. While 25 of the patients (78.12%) died, seven (21.87%) were discharged from the ICU. Candida parapsilosis (43.7%) was the opportunistic fungal agent isolated from most blood samples taken from COVID-19 positive patients. The mortality rate of COVID-19 positive patients with candidemia was 80%. While two out of the three patients (66.6%) for whom fungi were grown from their tracheal aspirate died, one patient (33.3%) was transferred to the ward. Opportunistic fungal infections increase the mortality rate of COVID-19-positive patients. In addition to the risk factors that we cannot change, invasive procedures should be avoided, constant blood sugar regulation should be applied, and unnecessary antibiotics use should be avoided.
Kiai dan Pendidikan Toleransi di Pesantren
Authors: Fajriyah Fajriyah , Rahman Mufiqur , Mo'tasim Mo'tasim , Hairit Artamin , Sayyi Ach. , Afandi Afandi , Rahmawati Roro Kurnia Nofita 
Published: September 2021

The kiai as a designer, implementer and controller of the course of education in the pesantren. Kiai determines the direction of a boarding school education. The attitude and character of the kiai determine the color of the pesantren education. This study is to describe particularly a role of Kiai in pesantren management patterns by promoting tolerance education as one of the Kiai's leadership styles in the pesantren. This research method uses qualitative methods with case studies. The data network was carried out by observation, interviews, documentation, and Focus Group Discussion (FGD). While data analysis using data reduction, display and conclusion. As well this study found a new model of tolerance education which a model of kiai cantered or a Model of kiai. First kiai became advisor and planner to set a education of tolerance based on multicultural view. Second, learning of tolerance delivered in the class integrated with the all the subjects, teachers should stated about tolerance during the class. And then the practice of tolerance accommodated by kiai including the community and santri activities as well kiai welcomed the tradition of community.
FOXO4 Inhibits the Migration and Metastasis of Colorectal Cancer by Regulating the APC2/β-Catenin Axis
Authors: Sun Yan , Wang Lin , Xu Xuehu , Han Puqing , Wu Jinghao , Tian Xuan , Li Mingsong 
Published: September 2021

Objective: Adenomatous polyposis coli 2 (APC2) is a colorectal cancer (CRC) tumor-suppressor gene. The progression of several kinds of cancer is closely associated with Forkhead box O4 (FOXO4). However, the function of FOXO4 in CRC is unclear. This study focused on the role of FOXO4 and the relationship between FOXO4 and APC2 in CRC migration and metastasis.Methods: The expressions of FOXO4, APC2, and p(S37)-β-catenin were detected in CRC tissues by immunohistochemistry, and their correlation was analyzed using the Spearman coefficient. Chromatin immunoprecipitation was used to test whether FOXO4 binds and regulates APC2 as a transcription factor. Either FOXO4 overexpression or APC2 knockdown was performed in CRC cell lines. The roles of FOXO4 and APC2 were investigated in CRC migration and metastasis.Results: FOXO4 was downregulated in CRC tissues compared with normal tissues and positively correlated with APC2 and p(S37)-β-catenin. FOXO4 could combine the promoter region of APC2 to upregulate its expression and increase the phosphorylated degradation of β-catenin. Stemness genes (CD133, ABCG1, and SOX2) were inhibited by FOXO4 overexpression in SW620 and HCT116 cell lines. Overexpressed FOXO4 suppressed epithelial–mesenchymal transition and the migration of CRC cell lines and metastasis of HCT116 in both the spleen and liver of nude mice, which was reversed by APC2 knockdown.Conclusion: This research demonstrates that overexpressed FOXO4 inhibits the migration and metastasis of CRC cells by enhancing the APC2/β-catenin axis, suggesting that FOXO4 is a potential therapeutic target of CRC.
Stereotactic and Robotic Minimally Invasive Thermal Ablation of Malignant Liver Tumors: A Systematic Review and Meta-Analysis
Authors: Tinguely Pascale , Paolucci Iwan , Ruiter Simeon J. S. , Weber Stefan , de Jong Koert P. , Candinas Daniel , Freedman Jacob , Engstrand Jennie 
Published: September 2021

BackgroundStereotactic navigation techniques aim to enhance treatment precision and safety in minimally invasive thermal ablation of liver tumors. We qualitatively reviewed and quantitatively summarized the available literature on procedural and clinical outcomes after stereotactic navigated ablation of malignant liver tumors.MethodsA systematic literature search was performed on procedural and clinical outcomes when using stereotactic or robotic navigation for laparoscopic or percutaneous thermal ablation. The online databases Medline, Embase, and Cochrane Library were searched. Endpoints included targeting accuracy, procedural efficiency, and treatment efficacy outcomes. Meta-analysis including subgroup analyses was performed.ResultsThirty-four studies (two randomized controlled trials, three prospective cohort studies, 29 case series) were qualitatively analyzed, and 22 studies were included for meta-analysis. Weighted average lateral targeting error was 3.7 mm (CI 3.2, 4.2), with all four comparative studies showing enhanced targeting accuracy compared to free-hand targeting. Weighted average overall complications, major complications, and mortality were 11.4% (6.7, 16.1), 3.4% (2.1, 5.1), and 0.8% (0.5, 1.3). Pooled estimates of primary technique efficacy were 94% (89, 97) if assessed at 1–6 weeks and 90% (87, 93) if assessed at 6–12 weeks post ablation, with remaining between-study heterogeneity. Primary technique efficacy was significantly enhanced in stereotactic vs. free-hand targeting, with odds ratio (OR) of 1.9 (1.2, 3.2) (n = 6 studies).ConclusionsAdvances in stereotactic navigation technologies allow highly precise and safe tumor targeting, leading to enhanced primary treatment efficacy. The use of varying definitions and terminology of safety and efficacy limits comparability among studies, highlighting the crucial need for further standardization of follow-up definitions.
The Influence of Transaction Process With Doctors on Patient Satisfaction, Self-Rating Anxiety and Self-Efficacy Among International Students in China
Authors: Julien Janelle , Wang Xuemei , Meng Han , Qian Zhou , Wang Dan , Zhang Xinping 
Published: September 2021

Objectives:To investigate the communication mechanism between international students and Chinese physicians by evaluating the influence of the transaction process on patient satisfaction, self-rating anxiety and self-efficacy.Methods:A cross-sectional survey was conducted among international students living in Central, Northern and Eastern China; enrolled at Chinese universities and experienced outpatient and inpatient healthcare services. Guided by the elements of King's transaction process: IR, Initiating and Responding; IP, Identifying Problems; MGS, Mutual Goal Setting; and EM, Exploring means and agreeing on means to achieve goals. We used spearman correlation analysis to calculate the correlation of the variables: patient satisfaction, self-efficacy, transaction process, IR, IP, MGS, and EM and regression analysis to measure the influence of transaction process on patient satisfaction, self-rating anxiety and self-efficacy.Results:Four hundred and four (404) participants were investigated for this study. The results of correlation analysis showed that there was a significant positive correlation among patient satisfaction, self-efficacy, transaction process, IR, IP, MGS, and EM (p&lt; 0.05). Regression analysis showed that the higher scores of IR (β = 0.176,p= 0.003) and MGS (β = 0.249,p= 0.002) was associated with the higher score of patient satisfaction; the higher the score of IR and IP was associated with the higher self-efficacy score (β = 0.148,0.225;p= 0.016,0.001); and higher the MGS score was associated the lower the self-rating anxiety (β = −0.220,p= 0.022).Conclusion:The influence of transaction process on patient satisfaction, self-rating anxiety and self-efficacy between Chinese physicians and international students (Patients) was established. Findings support the urgent implementation of tools at healthcare facilities to improve the communication between Chinese doctors and international students, therefore improving patient satisfaction and self-efficacy, and reducing anxiety.
Türkiye’de Yaşayan Suriyeli Mülteci Transtibial ve Transfemoral Amputelerde Protez Memnuniyetinin Değerlendirilmesi: Çok Merkezli Çalışma
Authors: KABLAN Nilüfer , VAROL Fatmagül , TATAR Yaşar 
Published: September 2021

Amaç: Bu çalışma uluslararası bir sivil toplum örgütü aracılığıyla Türkiye’de protez hizmeti verilen Suriye savaşı mağduru transtibial ve transfemoral amputelerin protez memnuniyetlerini değerlendirmek amacıyla yapılmıştır.
Yöntem: Araştırma Mart, 2019- Eylül,2020 tarihleri arasında xxxx Derneği Protez merkezlerinden protez hizmeti alan Suriye Savaşı mağduru unilateral transtibial (n=55) ve transfemoral (n=57) amputeler üzerinde gerçekleştirilmiştir. Protezle ilişkili memnuniyet değerlendirilmesi Orthotics Prostetics Users Survey- Cihaz Memnuniyeti Modülü (OPUS-CMM) ile yapılmıştır. Verilerin analizinde tanımlayıcı istatistiksel teknikler (ortalama ve standart sapma), ikili karşılaştırmalarda Bağımsız t Test, çoklu karşılaştırmalarda Kruskal Wallis Test kullanılmıştır.
Bulgular: Amputelerin cinsiyet, amputasyon seviyesi, amputasyon yılı, amputasyon nedeni, protez kullanım süresi ve süspansiyon tipine göre oluşturan alt gruplardaki OPUS-CMM puanlarının benzer olduğu görülmüştür (p>0,05).
Sonuç: Çalışılan bu gruplarda amputasyon seviyesi, cinsiyet, amputasyon yılı, protez kulllanım süresi ile süspansiyon tipinin protez memnuniyetini belirleyici bir etken olmadığı tespit edilmiştir.
Evaluation of the Level of Vitamin D and Angiopoetic Factors in Preeclampsia
Authors: AYDIN Sükeynet , DEVECİ ÖZKAN Asuman , USLU YUVACI Hilal , TUNCER Fatıma Betül , AKDOĞAN Mehmet 
Published: September 2021

Objective: Preeclampsia (PE), a pregnancy complication whose pathogenesis is still not fully clarified. Studies in the literature evaluating vitamin D deficiency more frequently in women with PE have conflicting results. Considering these contradictions, we aimed to evaluate the level and predictable potential of Vitamin D and angiogenic factors causing many disorders in maternal deficiency/insufficiency in women with PE.
Materials and Methods: Thirty-one (31) women diagnosed with PE and thirty-two (32) healthy pregnant women were included in this study. Vitamin D levels were measured by the chemiluminescence immunoassay (CLIA) TNF-α and PLGF levels by ELISA and PCT levels by electrochemiluminescence assay (ECLIA) method. ROC curves were created to determine the role of biochemical parameters in the prediction of PE.
Results: Vitamin D and PLGF levels were found lower and TNF-α and PCT levels were found higher in the PE group compared to the control group. The area under the ROC was 0.814, 0.7681, 0.8201, 0.9251 for gestational week (GW), Vitamin D, TNF-α and PLGF, respectively.
Conclusion: Vitamin D, TNF-a, PLGF values are consistent with the literature and they could be important markers in the prediction of PE. Our data revealed that these markers, which are already routinely analyzed, do not require an additional cost and time. Thereby, women with PE could be identified and followed up closely
The effect of vitamin E supplements added to Clomiphene citrate treatment on fertility in polycystic ovary syndrome
Authors: KANZA GÜL Derya , ŞOLT Ayça 
Published: September 2021

Objective: This study was designed to investigate the role of vitamin E supplementation during cycles in ovulation induction with clomiphene citrate (CC) in women with infertile Polycystic Ovary Syndrome (PCOS) on ovulation defect, endometrium thickness, clinical and ongoing pregnancy increase.
Materials and Methods: In this retrospective case control study, PCOS patients admitting to Yenibosna Private Nisa Hospital Obstetrics Clinic between January 1st 2015 and October 1st 2020 for infertility treatment were evaluated. A total of 306 patients were evaluated, including 139 Patients with PCOS receiving CC and vitamin E treatment (Vitamin E group), and 167 patients with PCOS who received CC treatment only (Clomiphene group). The primary result was that the cumulative ovulation rate (i.e. the rate of the cycles in which ovulation occurs during the entire follow-up period), the secondary result was the number of dominant follicles, the endometrial thickness on the day of HCG admission, and the clinical and ongoing pregnancy rates.
Results: No statistical differences were detected between the two groups in terms of dominant follicle counts, ovulation rates, and clinical and ongoing pregnancy rates (p>0.05). When the vitamin E group and control group were compared it was determined that there was a statistically significant difference between the groups in terms of endometrial thickness (p
Manisa Celal Bayar Üniversitesi Hafsa Sultan Hastanesi Adli Tıp Polikliniği’ne 2015-2019 Arasında Başvuran Kadın Adli Olgularımızın Değerlendirilmesi
Authors: KARABAĞ Gökmen , YAVUZ Mehmet Sunay , AKIN Ufuk , AYDIN Faruk , AYDIN Yusuf 
Published: September 2021

Amaç: Kişilerin beden veya ruh sağlığında bozulmaya hatta ölümle sonuçlanmasına neden olabilen dış etkenlerle meydana gelen her türlü olay adli olgu olarak kabul edilmektedir. Modernleşmenin bir etkisi olarak kadınların iş hayatına katılımı ve ev dışında geçirdikleri zamanın artmasıyla dış etkenlere maruz kalma ihtimallerinin yükselmesi sonucu kadınların adli olgu olarak daha sık oranda karşımıza çıkmasına neden olmaktadır. Çalışmamızda adli rapor düzenlenmek üzere kliniğimize yönlendirilen kadın olgular incelenerek, adli nitelikli kadın olguların değerlendirilmesi amaçlanmıştır.
Gereç ve Yöntem: 01.01.2015-31.12.2019 tarihleri arasında Anabilim Dalımıza adli rapor düzenlenmesi amacıyla yönlendirilen 443 kadın olgu, geriye dönük olarak değerlendirilerek; yaş, olay türü, yaralanmanın niteliği, olayın gerçekleşme zamanı ile polikliniğe müracaat zamanı ve adli rapor sonuçları yönünden incelenmiştir.
Bulgular: Anabilim Dalımıza beş yıllık süre içerisinde başvuran 1450 olgunun %30,6 (n=443)’sı kadın olup, yaş ortalaması 29,36±15,91 bulunmuştur. Olay türleri incelendiğinde; en sık %35,2 ile trafik kazası, ikinci sıklıkta ise %30,2 ile etkili eylem olgularının olduğu görülmüştür.
Sonuç: Çalışmamızdaki beş yıllık süre içerisinde polikliniğimize başvuruların yaklaşık üçte birini kadınlar oluşturmaktadır. Ülkemiz nüfusunun yaklaşık yarısını oluşturan kadın bireylerin, azımsanamayacak oranda, travmalar nedeniyle mağdur olmaları, bireysel ve toplumsal kayıplar, maddi ve manevi zararlar oluşturmaktadır. Bu zararların önlenmesinde; koruyucu hekimliğin gereği olarak bireysel dikkat göstermek, toplumsal önlemlerin alınmasına kılavuzluk etmek, olaylar meydana geldiğinde de olguların adli tıbbi değerlendirmelerin hızlı ve eksiksiz bir şekilde yapılarak adli sürecin işlemesi son derece önem taşımaktadır.
Karpal Tünel Sendromunun Cerrahi Tedavisinde Sınırlı İnsizyon Uygulamalarımız
Authors: AHMEDOV Anvar 
Published: September 2021

Amaç: Klasik açık teknik ile yapılan karpal tünel ameliyatlarında, uygulanan insizyona bağlı olarak, ameliyat sonrası pilar bölgede ağrı ve hassasiyet, el bileğinde sertlik, palmar kütanöz dal kesilmesine bağlı nöroma şikayetleri görülmektedir. Ameliyat insizyonunun sınırlı tutularak, pilar bölgesinin belirli derecede korunduğu olgularda, bahsedilen şikayetlerin azaltılacağı düşündüğümüz için bu çalışma planlandı.
Gereç yöntem: 2010 Ocak -2011 Aralık tarihleri arasında kliniğimizde seçilmiş karpal tünel sendromu tanısı alan 40 hastaya, sınırlı insizyonla karpal tünel cerrahi tedavisi uygulandı. Hastalarda preoperatif EMG ile sinirlerin hasar derecesine bakıldı. Disabilities of the Arm, Shoulder and Hand-Türkçe semptom skorlaması (Quick DASH-T) ve Boston Karpal Tünel Sorgulama Anketi (BKTSA) kullanılarak hastalığın şiddeti ve üst ekstremitenin fonksiyonel durumu değerlendirildi. Tüm hastalarda Tinel ve Phalen testleri yapıldı.
Bulgular: Postoperatif dönemdeki takiplerde hastalar değerlendirildi. Pilar bölgesinde ağrı ve hasiyet hiçbir hastada görülmedi. Hiçbir hastada skar sertliği şikayeti ve nöroma gelişimine rastlanmadı. Tinel ve Phalen testlerinin postoperatif negatiflik oranı literatür verilerine yakın olarak hesaplandı. Quick DASH-T ve BKTSA sonuçları preoperatif sonuçlarla karşılaştırıldı ve istatistiksel olarak anlamlı bulundu. Literatür ve benzer yayınlarda değerlendirilmesi önerilen yüzeyel ark yaralanmasının tespiti amacıyla 5 kişilik randomize grup oluşturuldu. Yapılan ultrason incelemesinde hiçbir hastada yüzeyel palmar ark hasarına rastlanmadı.
Sonuç: Çalışmamız sonucunda, uygun olgularda sınırlı insizyon uygulanarak yapılan karpal tünel cerahisininin olumlu sonuçlar verdiği, özellikle mesleği gereği pilar bölgesine bası uygulanan olgularda tercih edilebilecek bir yöntem olabileceği kanaatine varıldı.