bluuebunny/crossref_metadata_2025_split huggingface dataset:
Ex-situ biogas upgrading to methane and removal of VOCs in a system of zero valent iron and anaerobic granular sludge
Authors: Lytras Nikolaos , Andronikou Maria , Chrysanthou Georgia , Stylianou Marinos , Agapiou Agapios , Vyrides Ioannis 
Published: June 2021

Abstract
In the current study, a novel process of ex-situ biogas upgrading to biomethane is presented which is based on a system consisted of anaerobic sludge and zero valent iron (ZVI). The ZVI when is added in an aquatic system with anaerobic granular sludge generates H2 under anaerobic abiotic conditions. Then, the H2 and CO2 are converted by the hydrogenotrophic methanogens to CH4. Biogas upgrading to biomethane was achieved in 4 days in a system of anaerobic granular sludge, 50 g L1 ZVI initial pH 5 and 20 g L1 NaHCO3. In this system when zero valent scrap iron (ZVSI) was added instead of ZVI required longer period (21 days) to achieve biogas upgrading. Volatile organic compounds (VOCs) analysis in raw biogas (in system of anaerobic sludge and ZVI) showed mainly the reduction of low mass straight- and branched-chain alkanes (C6-C10); however, no other particular trend regarding the removal of other VOCs was observed. H2S and NH3 were found to be substantially reduced, when the anaerobic sludge was exposed to ZVI compared to no decrease in serum bottles free of ZVI.
A 5-mRNA-Based Prognostic Signature of Survival in Lung Adenocarcinoma
Authors: xia qianlin , Yu Weimo , Li Qiuyue , Wang Jin , Du Yuzhen 
Published: June 2021

Abstract
Background: Lung adenocarcinoma (LUAD) is the most common non-small cell lung cancer, with an increasing incidence and poor prognosis. To evaluate the prognosis of LUAD patients and optimize treatment, effective clinical research prediction models are urgently needed. Methods : In this study, we thoroughly mined LUAD genomic data from GEO (GSE43458, GSE32863, and GSE27262) and TCGA datasets, including 698 LUAD and 172 healthy (or adjacent normal) lung tissue samples. Single-factor Cox and LASSO regression analyses were used to screen DEGs related to patient prognosis, and multivariate Cox regression analysis was applied to establish the risk score equation and construct the survival prognosis model. Receiver operating characteristic (ROC) curve and Kaplan-Meier (KM) survival analyses with clinically independent prognostic parameters were performed to verify the predictive power of the model and further establish a prognostic nomogram. Results: A total of 380 DEGs were identified in LUAD tissues through GEO and TCGA datasets, and 5 DEGs (TCN1, CENPF, MAOB, CRTAC1 and PLEK2) were screened out by multivariate Cox regression analysis, indicating that the prognostic risk model could be used as an independent prognostic factor (HR = 1.520, P < 0.001). Internal and external validation of the model confirmed that the prediction model had good sensitivity and specificity (AUC = 0.754, 0.737). Combining genetic models and clinical prognostic factors, nomograms can also predict overall survival more effectively. Conclusion: A 5-mRNA-based model was constructed to predict the prognosis of lung adenocarcinoma, which may provide clinicians with reliable prognostic assessment tools and help clinical treatment decisions.
The Effect of Four Different Single Nucleotide Polymorphisms on Coronary Heart Disease in a Han Chinese Population in Xinjiang Region
Authors: Abudureyimu Shajidan , Abulaiti Palida , Xing Zhi , Li Hui , Liu Sha Sha , Li Wen , Gao Ying 
Published: June 2021

Abstract
Background: In this current work we aimed to find the effect of four different single Nucleotide Polymorphisms(SNPs) rs1122608(SMARCA4)，rs2230806(ABCA1), rs12563308（ANGPTL3）,and rs662799（APOA5 ) on coronary heart disease(CHD) in a Han Chinese population in Xinjiang region of China. Methods: This study involved 914 subjects with 493 CHD patients and 421 healthy controls. The genotype distribution of analyzed and their relations with CHD risk factors were assessed.Results: No statistical differences were found in genotype and allele distributions of above SNPs between CHD and healthy controls (P>0.05). Serum level of high-density lipoprotein cholesterol (HDL-C) was higher in in TT genotype of rs1122608 when compared with GT and GG genotypes (P< 0.01) in CHD patients ; serum triglyceride(TG) level was higher in rs662799 GG genotype than GA and AA genotypes(P <0.00); AA of rs662799 was associated with higher HDL-C level compared with other two genotypes (P < 0.01) ; rs12563308 and rs223086 were not associated with any serum lipid traits (P > 0.05 for all). Logistic regression analysis showed that the SNPs examined were not related to CHD (p > 0.05). Also no association was found between four SNPs with the angiographic severity of CHD patients (p > 0.05). Conclusions: APOA5 rs662799 GG allele is associated with elevated triglyceride and might act as a risk factor for CHD; SMARCA4 rs1122608 TT allele and APOA5 rs662799 AA allele are associated with elevated high-density lipoprotein cholesterol levels, and might play a protective role in the development of CHD.
Human leucocyte antigen G (HLA-G) and its murine homologue Qa-2 protect from pregnancy loss
Authors: Dietz Stefanie , Schwarz Julian , Velic Ana , Menendez Irene Gonzalez , Quintanilla-Martinez Leticia , Casadei Nicolas , Marmé Alexander , Poets Christian , Gille Christian , Köstlin-Gille Natascha 
Published: June 2021

Abstract
During pregnancy, the maternal immune system has to balance tightly between protection against pathogens and tolerance towards a semi-allogeneic organism. Dysfunction of this immune adaptation can lead to severe complications such as pregnancy loss, preeclampsia or fetal growth restriction. The MHC-Ib molecule HLA-G is well known to mediate immunological tolerance. However, no in-vivo studies have yet demonstrated a beneficial role of HLA-G for pregnancy success. Myeloid derived suppressor cells (MDSC) are suppressively acting immune cells accumulating during pregnancy and mediating maternal-fetal tolerance. Here, we analyzed the impact of Qa-2, the murine homologue to HLA-G, on pregnancy outcome in vivo. We demonstrate that lack of Qa-2 led to intrauterine growth restriction and increased abortion rates especially in late pregnancy accompanied by changes in uterine gene expression, altered spiral artery remodeling and protein aggregation in trophoblast cells indicating a preeclampsia-like phenotype. Furthermore, lack of Qa-2 caused decreased accumulation of MDSC and impaired MDSC function. Lastly, we show that application of sHLA-G reduced abortion rates in Qa-2 deficient mice by inducing MDSC. Our results highlight the importance of an interaction between HLA-G and MDSC for pregnancy success and the therapeutic potential of HLA-G for the treatment of immunological pregnancy complications.
Comparison Between Sevoflurane and Propofol With Remifentanil Anesthesia Avoid Neuromuscular Blocking Agent After Intubation in Thyroid Operation
Authors: Yang Chunyao , Sun Jing , Lu Xihua , Yang Chen 
Published: June 2021

Abstract
Background: The goal of the study is to compare sevoflurane and propofol with remifentanil anesthesia avoid neuromuscular blocking agent after intubation in thyroid operation with intra-operative neuromonitoring.Methods: 80 patients scheduled to receive thyroid operation were randomly assigned to sevofluraneor propofol anesthesia maintained group. The time of rocuronium recovery profile were monitored. Adverse events such as sinus bradycardia, tachycardia, hypertension, hypotension and movement were recorded. Analyze the time from anesthetic stop to the extubation time and operation time. Record the incidence rate of sore throat, drowsiness, agitation, nausea and vomiting after extubation. Record the first neuromonitoring time and the number of successful neuromonitoring.Results: There were significant differences in the recovery profile of rocuronium between sevoflurane and propofol group(P<0.05). The incidence of hypotension was similar between sevoflurane and propofol group. There was no significant difference of sinus braducardia between the two groups. The incidence of movement was less in sevoflurane compared to propofol(12.% vs. 47.5%, P=0.002). The propofol group had longer extubation time than sevoflurane group(13.10±1.52 vs. 8.07±1.07 min, P=0.001).The incidence of sore throat, drowsiness, agitation, nausea and vomiting in the two groups was similar during the recovery period, and the difference was not statistically significant. There was no significant difference of neuromonitoring between the two groups.Conclusion: Sevoflurane has advantage with less movement adverse effects in thyroid surgery with intra-operative neuromonitoring compared to propofol when associated with remifentanil 0.1μg/kg/min in general anesthesia.Trial registration: Chinese Clinical Trial Registry (ChiCTR1800017166, 15 July 2018)
Canagliflozin Protects The Cardiovascular System Through Effects On The Gut Environment In Non-Diabetic Nephrectomized Rats
Authors: Matsui Ayumi , Yoshifuji Ayumi , Irie Junichiro , Tajima Takaya , Uchiyama Kiyotaka , Itoh Tomoaki , Hasegawa Kazuhiro , Kanda Takeshi , Tokuyama Hirobumi , Wakino Shu , Itoh Hiroshi 
Published: June 2021

Abstract
The gut produces toxins that contribute to the cardiovascular complications of chronic kidney disease. Canagliflozin, a sodium glucose cotransporter (SGLT) 2 inhibitor that is used as an anti-diabetic drug, has a weak inhibitory effect against SGLT1 and may affect the gut glucose concentration and environment. Here, we determined the effect of canagliflozin on the gut microbiota and the serum gut-derived uremic toxin concentrations in 5/6th nephrectomized (Nx) rats. Canagliflozin increased the colonic glucose concentration and restored the number of Lactobacillus bacteria, which was low in Nx rats. In addition, the expression of tight junction proteins in the ascending colon was low in Nx rats, and this was partially restored by canagliflozin. Furthermore, the serum concentrations of gut-derived uremic toxins were significantly increased by Nx and reduced by canagliflozin. Finally, the wall of the thoracic aorta was thicker and there was more cardiac interstitial fibrosis in Nx rats, and these defects were ameliorated by canagliflozin. The increases in colonic glucose concentration, Lactobacillus numbers, and tight junction protein expression; and the decreases in serum uremic toxin concentrations and cardiac interstitial fibrosis may have been caused by the inhibition of SGLT1 by canagliflozin because similar effects were not identified in tofogliflozin-treated rats.
Estimation Of Excessive Weight Loss In Bariatric Surgery Patients: A Prediction Model Retrospective Cohort Study
Authors: Hatami Mahsa , Pazouki Abdolreza , Kabir Ali 
Published: June 2021

Abstract
Background and Objective: Bariatric surgery has been recognized as the most effective long-term treatment for morbid obesity. Despite the considerable positive results, adverse consequence can develop. Excessive Weight Loss (EXWL), a rare consequence of bariatric surgery, can lead to a broad adverse consequence. The aim of this study was determining of prevalence and the predicting model of EXWL in patient underwent bariatric surgery until 24 months after surgery.Material and Methods: Data have been extracted from the National Obesity Surgery Database in obesity clinic of * University of Medical Sciences. The subjects of this retrospective cohort study were morbid obese individuals who underwent three various types of bariatric surgery (One Anastomosis Gastric Bypass (OAGB), Roux-en-Y gastric bypass (RYGB) or sleeve gastrectomy (SG)) in period of 24 months ago. EXWL has been defined as excess weight loss more than 100% at any time until 24 months after surgery. SPSS (version 23) was used in data analysis.Results: Among 4214 subjects of this study, most excess weight loss after surgery has taken place in 18 months after surgery. 18.5% (n=407) of patients experienced EXWL with highest percentage among OAGB patients (22.6%). The females (20.4% vs.9.9%) and younger persons (35.45 ± 10.25 vs. 39.06 ± 10.76) were more susceptible to EXWL. patients with EXWL had significantly lower BMI (body mass index) (41.11± 4.51 vs. 46.73±6.26) (Kg/m2), and were less probable to had emotional eating. Visceral fat level, fat percentage and BMI were the best predictor of EXWL (P-value for all <0.05).Conclusions: Surgery should be adjusted in younger females with a lower BMI and healthy metabolic status who are more prone to EXWL. In such a way that minimize weight loss speed/value. It may be possible by selection of other surgery procedures, rather than OAGB, tighter follow ups and consultations of patients after surgery is emphasized for more EXWL vulnerable patients.
Virucidal Efficacy of Guanidine-Free Inactivants and Rapid Test Buffers Against SARS-CoV-2: Implications for Risk Assessment of Diagnostic Procedures
Authors: Davies Katherine , Arnold Ulrike , Buczkowski Hubert , Burton Christopher , Welch Stephen R , Green Nicole , Strachan Rhea , Beetar-King Tracy , Spencer Peter , Hettiarachchi Nipunadi , Hannah Matthew J , Jones Matthew , Cane Patricia A , Bruce Christine B , Woodford Neil , Roberts Allen DG , Killip Marian J 
Published: June 2021

Abstract
A pathogen inactivation step during collection or processing of clinical samples has the potential to reduce infectious risks associated with diagnostic procedures. It is essential that these inactivation methods are demonstrated to be effective, particularly for non-traditional inactivation reagents or for commercial products where the chemical composition is undisclosed. This study assessed inactivation effectiveness of twenty-four next-generation (guanidine-free) nucleic acid extraction lysis buffers and twelve rapid antigen test buffers against SARS-CoV-2, the causative agent of COVID-19. These data have significant safety implications for SARS-CoV-2 diagnostic testing and support the design and evidence-based risk assessment of these procedures.
Selective pericentromeric heterochromatin dismantling caused by TP53 activation during senescence
Authors: Mendez-Bermudez Aaron , Pousse Mélanie , Lototska Liudmyla , Tessier Florent , Croce Olivier , Latrick Chrysa , Cherdyntseva Veronica , Nassour Joe , JIANG Xiaohua , LU Yiming , Abbadie Corinne , Gagos Sarantis , Ye Jing , Gilson Eric 
Published: June 2021

Abstract
Cellular senescence triggers various types of heterochromatin remodelling that contribute to aging. However the age-ralated mechanisms that lead to these epigenetic alterations remain elusive. Here, we asked how two key aging hallmarks, telomere shortening and consititutive heterochromatin loss, are mechanistically connected during senescence. We show that, at the onset of senescence, pericentromeric heterochromatin is specifically dismantled consisting of chromatin decondensation, accumulation of DNA breakages, illegitimate recombination and loss of DNA. This process is caused by telomere shortening or genotoxic stress by a sequence of events starting from p53-dependent downregulation of the telomere protective protein TRF2. The resulting loss of TRF2 at pericentromeres trigger DNA breaks activating ATM, which in turn leads to heterochromatin decondensation by releasing Kap1 and Lamin B1, recombination and satellite DNA excision found in the cytosol associated to cGAS. This TP53-TRF2 axis activates the interferon response and the formation of chromosome rearrangements when the cells escape the senescent growth arrest. Overall, these results reveal the role of p53 as pericentromeric disassembler and define the basic principles of how a TP53-dependent senescence inducer hierarchically leadns to selective pericentromeric dismantling through the downregulation of TRF2.
The Effect of Abrasive Particles Movement on Deburring Effectiveness in REMF Process by using Explicit Dynamic Simulations
Authors: Lee Jung-Hee , Lee Se-Yeong , Kwak Jae-Seob 
Published: June 2021

Abstract
The main aim of this study was to suggest rotating electro-magnetic finishing(REMF) which had the capability to yield ultra-fine finished surface in micro-meter level with high productivity in a short time. The performance of the REMF process was primarily dependent upon magnetic and impact energy by the physical properties of a particle, particle velocity, and impact angle. Therefore, in this study, numerical simulations were conducted by explicit dynamic analysis to verify the effect of both single and multiple abrasive media impact characteristics on a deburring area of the machined surface. Based on the observed results and Pareto analysis, it was found that the overall weight of the particles was the most significantly affected for burr reduction, and deburring area was proportional to the increasing impact angle as well. In addition, to estimate the relationship between process factors and output variable and determine the optimum condition for burr reduction, a second-order polynomial model was developed by adopting a response surface methodology. As a result, the predictive model agreed with numerical analyzed results with 84.4% of the accuracy rate. The optimized deburring area was 2.27mm2 at 1,800rpm of rotational velocity, 0.7mm of particle diameter, and 2.0kg of total particle weight. It was founded to be similar to the maximum deburring area, about 2.37mm2, obtained from the simulations at the same condition.
Lung Damage among Individuals with Biomass Smoke Exposure Characterized by CT Features and Changes in Pulmonary Function
Authors: Chen Jinglong , Jiang Changbin , Zheng Youlan , Zhao Dongxing , Wu Fan , Zhao Zhuxiang , Zhao Jun , Li Qin , Li Bing , Peng Gongyong , Zhou Yumin , Ran Pixin 
Published: June 2021

Abstract
BackgroundBiomass smoke exposure (BSE) is an important etiological factor in chronic obstructive pulmonary disease (COPD). However, few studies have focused on the effects of BSE in the respiratory muscles or lungs.MethodsUsing a cohort, we selected 98 participants in underdeveloped rural areas: 16 healthy individuals with BSE (“BSE normal”), 19 patients with BSE and COPD (“BSE+COPD”), 13 healthy individuals with cigarette smoke exposure (“CSE normal”), 25 patients with cigarette smoke exposure and COPD (“CSE+COPD”), and 25 healthy controls. Patients with GOLD stage I and II COPD were included. Baseline data (demographic data, BSE or CSE, lung function, and CT findings) and follow-up lung function data were collected. CT parameters of emphysema, pulmonary small vessels, airway remodeling, pectoralis muscles, and erector spinae muscle were measured.ResultsIndividuals with BSE were mainly women (32/35, 91.43%). Compared with the CSE+COPD group, the BSE+COPD group demonstrated slow lung function decline, increased lower lung emphysema, and narrower airway lumen and airway wall thickening in moderate and small airways (all P<.05). Compared with healthy controls, the CSE normal and BSE normal groups exhibited significant reduction of pulmonary small vessel area and obvious airway remodeling in small airways (P<.05). Compared with the BSE normal group, the BSE+COPD group showed significantly more severe emphysema and airway remodeling, as well as reduced left pectoralis major muscle area (all P<.05).ConclusionsHealthy individuals with BSE had reduced pulmonary small vessel area and obvious airway remodeling; patients with BSE and COPD showed more severe emphysema, airway remodeling, and pectoralis major muscle change. More investigations are needed regarding interventions for BSE.Trial registration: ChiCTR, ChiCTR-OO-14004264 . Registered 12 February 2014, http://www.chictr.org.cn/showproj.aspx?proj=5304
Validation of New Cine Magnetic Resonance Imaging Parameters For The Differential Diagnosis of Chronic Idiopathic Intestinal Pseudo-Obstruction
Authors: Sato Hiroki , Ogiwara Hiroyuki , Takahashi Kazuya , Kawata Yuzo , Kojima Yuichi , Tominaga Kentaro , Yokoyama Junji , Hamamoto Yoshihiko , Terai Shuji 
Published: June 2021

Abstract
Chronic idiopathic pseudo-obstruction (CIPO) is a severe and refractory intestinal motility disorder whose diagnosis currently relies on subjective imaging assessments. Cine magnetic resonance imaging (MRI) may potentially improve the quantitative analysis of gastrointestinal motility; however, suitable CIPO detection parameters should be determined.Cine MRI was performed in seven patients with CIPO and 11 healthy controls. The logarithm of the Mahalanobis distance (x1) and luminal distance variation per time (x2) were used as the original parameters to determine CIPO diagnostic thresholds. Furthermore, the correlation between cine MRI findings and CIPO severity was investigated.Threshold values of α=1.10 and β=0.15 for x1 and x2, respectively, produced a CIPO diagnosis sensitivity of 1.00 (7/7) and specificity of 0.82 (9/11). The resulting error was 0.11 (2/18). The two parameters were correlated (Pearson’s correlation coefficient: -0.52). The intestinal tracts of patients with severe CIPO requiring home parenteral nutrition belonged to the category defined by x1 ≥1.10 and x2 ≤0.15.Cine MRI is effective for the quantitative evaluation of small intestinal motility and CIPO diagnosis when using the abovementioned parameters and can be useful for treatment decision-making. However, these parameters have a wide distribution in healthy volunteers; this may complicate the detection of other disorders.