bluuebunny/crossref_metadata_2025_split huggingface dataset:
Quantitative Susceptibility Atlas Construction in Montreal Neurological Institute Space: Towards Histological-consistent Iron-rich Deep Brain Nucleus Sub-region Identification
Authors: He Chenyu , Guan Xiaojun , Zhang Weimin , Li Jun , Liu Chunlei , Wei Hongjiang , Xu Xiaojun , Zhang Yuyao 
Published: November 2021

Abstract
Iron-rich deep brain nuclei (DBN) of the human brain are involved in various motoric, emotional and cognitive brain functions. The abnormal iron alterations in the DBN are closely associated with multiple neurological and psychiatric diseases. Quantitative susceptibility mapping (QSM) provides the spatial distribution of tissue magnetic susceptibility in the human brain. Compared to traditional structural imaging, QSM has superiority for imaging the iron-rich DBN owing to the susceptibility difference existing between brain tissues. In this study, we construct a Montreal Neurological Institute (MNI) space unbiased QSM human brain atlas via group-wise registration from 100 healthy subjects aged 19-29 years. The atlas construction process is guided by hybrid images that fused from multi-modal Magnetic Resonance Images (MRI), thus named as Multi-modal-fused magnetic Susceptibility (MuSus-100) atlas. The high-quality susceptibility atlas provides extraordinary image contrast between iron-rich DBN with their surroundings. Parcellation maps of DBN and their sub-regions that are highly related to neurological and psychiatric pathology are then manually labeled based on the atlas set with the assistance of an image border-enhancement process. Especially, the bilateral thalamus is delineated into 64 detailed sub-regions referring to the Schaltenbrand and Wahren stereotactic atlas. To our best knowledge, the histological-consistent thalamic nucleus parcellation map is well defined for the first time in MNI space. Comparing with existing atlases emphasized on DBN parcellation, the newly proposed atlas outperforms on atlas-guided individual brain image DBN segmentation accuracy and robustness. Moreover, we apply the proposed DBN parcellation map to conduct detailed identification of the pathology-related iron content alterations in subcortical nuclei for Parkinson Disease (PD) patients. We envision that the MuSus-100 atlas could play a crucial role in improving the accuracy of DBN segmentation for the research of neurological and psychiatric disease progress and also be helpful for target planning in deep brain stimulation surgery
Engineering Saccharomyces Cerevisiae for the Production and Secretion of Affibody Molecules.
Authors: Gast Veronica , Sandegren Anna , Dunås Finn , Ekblad Siri , Güler Rezan , Thorén Staffan , Mohedano Marta Tous , Molin Mikael , Engqvist Martin K M , Siewers Verena 
Published: November 2021

Abstract
BackgroundAffibody molecules are synthetic peptides with a variety of therapeutic and diagnostic applications. To date, Affibody molecules have mainly been produced by the bacterial production host Escherichia coli. There is an interest in exploring alternative production hosts to address if improvements in terms of yield, ease of production and if purification advantages can be identified. In this study, we evaluated the feasibility of Saccharomyces cerevisiae as a production chassis for this group of proteins. Results We examined the production of three different Affibody molecules in S. cerevisiae and found that these Affibody molecules were partially degraded. An albumin-binding domain, which may be attached to the Affibody molecules to increase their half-life, showed to be a substrate for several S. cerevisiae proteases. We tested the removal of three vacuolar proteases, proteinase A, proteinase B and carboxypeptidase Y. Removal of one of these, proteinase A, resulted in intact secretion of one of the targeted Affibody molecules. Removal of either or both two additional proteases, carboxypeptidase Y and proteinase B, resulted in intact secretion of the two remaining Affibody molecules. The produced Affibody molecules were verified to bind human HER3 as potently as the corresponding molecules produced in E. coli in an in vitro surface-plasmon resonance binding assay. Finally, we performed a fed-batch fermentation with one of the engineered protease-deficient S. cerevisiae strains and achieved a protein titer of 530 mg Affibody molecule/L. ConclusionThis study shows that engineered S. cerevisiae has a great potential as a production host for recombinant Affibody molecules, reaching high yields and for proteins where endotoxin removal could be challenging, the use of S. cerevisiae obviates the need for endotoxin removal from protein produced in E. coli.
Normalization of assembly technical process of the frame of power and control equipment for nuclear power plants by the method of simulation
Authors: Loskutov I. A. 
Published: November 2021

Objectives.The modern world is associated with a clear dependence on it. The number of consumers is growing exponentially. In order to minimize the costs of enterprises, an assumption was made about the rationality of the number of nuclear power plants. When considering the timing of the construction of nuclear complexes, an excessive duration of the manufacture of power and control equipment for nuclear power plants was revealed. In order to reduce time costs, it was proposed to standardize the work of assembly, assembly and packaging production associations.Method.Research in this area was practically not carried out and were, often, of an episodic nature. Initially, it was believed that the operations of the technological process of manufacturing equipment should be rationalized by classical methods, however, in the previous works of the author, a new approach was proposed, based on the analysis of the characteristics of the employee employed in the technical process, the specifics of the type of operational work, as well as various disturbing factors that negatively affect the production time. Then the systematization of the obtained data and their further modeling is carried out.Results. The paper shows the derivation of the final equation for the assembly of the frame of power and control equipment for nuclear power plants. The substantiation of the applied methods was given, the target audience was indicated, the regulatory framework was taken into account. In addition, the results of previous works are shown, on the basis of which the mathematical model was built. Separately, recommendations were given on the additional use of the functions of the resulting equation.Conclusion.The equation obtained in the work makes it possible to calculate with maximum accuracy the time spent on assembling the frame of the power and control equipment for a nuclear power plant. The principles shown in other works can be used in an area that has directionality with minimal model change.
Não violência, Cristianismo e Sociedade em O Reino de Deus está em Vós, de Tolstói
Authors: Martins de Souza César , Castro Weverton 
Published: November 2021

No final do século XIX, o renomado escritor russo Liev Tolstói publicou O reino de Deus está em vós, no qual defende suas principais propostas para a sociedade baseadas em sua visão sobre o cristianismo. Em momentos em que o cristianismo aparece na sociedade como sendo utilizado para embasar a defesa do endurecimento de punições contra o crime, como pena de morte, o uso de armas para se defender e até mesmo de guerras e exclusões sociais em todo o mundo, a proposta de Tolstói de libertar a humanidade do mal, baseando-se na resistência não violenta a todas formas de violência ganha atualidade. A análise de O reino de Deus está em vós, de Tolstói, evidencia sua defesa de que Cristo libertou todas as pessoas, e que podem então decidir entre o bem e o mal, rejeitando a violência e buscando estabelecer o reino de Deus em cada ser humano para que pudessem chegar ao entendimento da verdade. Esta busca não permitiria se submeter às ordens contrárias aos ensinamentos de Cristo, que para Tolstói, destacam de forma inegociável que os cristãos não devem fazer uso de todo e qualquer uso de violência, nem mesmo para reprimi-la.
ANALISIS PERGERAKAN SAHAM PERUSAHAAN SUB SEKTOR FARMASI MENGGUNAKAN INDIKATOR BOLLINGER BAND DI TENGAH PANDEMI COVID-19
Authors: Elita Intan , Wardianto K. Bagus , Harori M. Iqbal 
Published: November 2021

This study aims to measure the accuracy of technical analysis using the Bollinger Band indicator in predicting stock prices in the middle of pandemic covid-19. The concept in this study is to compare daily stock price predictions according to technical indicators with the closing prices that occured on that day. Sample selection technique used in this research used a purposive sampling method and obtained 9 pharmaceutical sub-sector companies listed on the IDX from February to April 2020. The type of data used is a chart of the company's daily stock price movements obtained from finance.yahoo.com. The data analysis technique used was the paired sample t-test and used the SPSS 26 analysis tool. The results of this study indicate that the Bollinger indicator does not have a significant difference.
ABSTRAK
Penelitian ini bertujuan untuk mengukur keakuratan analisis teknikal dengan indikator Bollinger Band dalam memprediksi harga saham pada masa pandemi Covid-19. Konsep pada penelitian ini adalah membandingkan prediksi harga saham harian menurut indikator teknikal dengan harga penutupan yang terjadi pada hari tersebut. Teknik pengambilan sampel dalam penelitian ini menggunakan metode purposive sampling dan diperoleh sebanyak 9 perusahaan sub sektor farmasi yang terdaftar di BEI selama Februari hingga April 2020. Jenis data yang digunakan yaitu berupa grafik pergerakan harga saham harian perusahaan yang diperoleh dari finance.yahoo.com. Teknik analisis data yang digunakan adalah uji independent sample t-test dan menggunakan alat analisis program SPSS 26. Hasil penelitian ini menunjukkan bahwa indikator Bollinger tidak memiliki perbedaan yang signifikan.
DAMPAK DIGITALISASI TERHADAP INDUSTRI SKINCARE
Authors: Sari Dila Komala , Sugiono Arif , Nugeraha Prasetya 
Published: November 2021

This study aimed to determine how much influence e-service quality, e-advertisement, and celebrity endorser had on online purchasing decisions at Bening's Clinic skincare. The population in this study were Bening's Clinic consumers. The data were obtained from questionnaires that were filled out online using a Likert scale,. The sampling technique used was purposive sampling technique with a total sample size of 385 respondents from Bening's Clinic consumers. The data analysis of this research used descriptive data analysis and multiple linear regression analysis. The results of this study indicated that e-service quality partially did not had a significant effect on purchasing decisions, e-advertisement and celebrity endorsers partially have a significant effect on online purchasing decisions at Bening's Clinic skincare. In this study, trust and response were dominant factors that need to be maintained, because they determined Bening's Clinic's online skincare purchase decision as a beauty product.

Abstrak
Penelitian ini bertujan untuk mengetahui seberapa besar pengaruh e-service quality, e- advertisement, dan celebrity endorser terhadap keputusan pembelian secara online pada skincare Bening’s Clinic. Populasi dalam penelitian ini adalah konsumen Bening’s Clinic. Data tersebut diperoleh dari kuesioner yang diisi secara online dengan menggunakan skala likert, . teknik pengambilan sampel menggunakan teknik purposive sampling dengan jumlah sampel sebanyak 385 responden konsumen Bening’s Clinic. Analisis data penelitian ini menggunakan analisis data deskriptif dan analisis regresi linear berganda.Hasil penelitian ini menunjukkan bahwa e-service quality secara parsial tidak berpengaruh signifikan terhadap keputusan pembelian, e- advertisement dan celebrity endorser secara parsial berpengaruh signifikan terhadap keputusan pembelian secara online pada skincare Bening’s Clinic. Pada penelitian ini kepercayaan dan respon menjadi faktor dominan yang perlu dipertahankan, karena menentukan keputusan pembelian online skincare Bening’s Clinic sebagai produk kecantikan.
Tuberculosis detection in nonhuman primates is enhanced by use of testing algorithms that include an interferon-γ release assay
Authors: Yee JoAnn L. , Prongay Kamm , Van Rompay Koen K. A. , Meesawat Suthirote , Kemthong Taratorn , Halley Bryson , Carpenter Amanda , Nham Peter , Rogers Kenneth , Hasselschwert Dana , Villinger Francois , Jay Alexandra N. , Warit Saradee , Malivijitnond Suchinda , Roberts Jeffrey A. 
Published: November 2021

Abstract

OBJECTIVE
To develop a testing algorithm that incorporates multiple assays to evaluate host cellular and humoral immunity and antigen detection concerning Mycobacterium tuberculosis complex (MTBC) infection in captive nonhuman primates.

ANIMALS
Cohorts of captive-bred and wild-caught macaques from 5 different geographic regions.

PROCEDURES
Macaques were tested for MTBC infection by use of a γ interferon tuberculosis (GIFT) assay, an interferon-γ release assay, and other assays. In the first 2 cohorts (n = 15 and 181), initial validation of the GIFT assay was performed by use of experimentally infected and unexposed control macaques. In the next 3 cohorts (n = 59, 42, and 11), results were obtained for opportunistically collected samples from macaques exposed during spontaneous outbreaks.

RESULTS
Sensitivity and specificity of the GIFT assay in the control cohorts were 100% and 97%, respectively, and were variable but enhanced by incorporating results from multiple assays in spontaneous outbreaks.

CLINICAL RELEVANCE
The detection and management of MTBC infection in captive nonhuman primate populations is an ongoing challenge, especially with animal imports and transfers. Despite standardized practices of initial quarantine with regular intradermal tuberculin skin testing, spontaneous outbreaks continue to be reported. Since infection encompasses a range of disease manifestations over time, a testing algorithm that incorporates multiple assays, such as the GIFT assay, to evaluate host cellular and humoral immunity in addition to agent detection is needed. Testing a combination of samples from controlled studies and spontaneous outbreaks of MTBC infection in nonhuman primates would advance the development and validation of a functional algorithm that incorporates promising tools such as the GIFT assay.
Preoperative serum C-reactive protein concentration can be used to detect gallbladder rupture in dogs with gallbladder mucocele
Authors: Asakawa Makoto , Fukuzawa Mayuko , Asakawa Midori Goto , Flanders James A. 
Published: November 2021

Abstract

OBJECTIVE
To determine whether serum C-reactive protein (CRP) concentration could be used to detect gallbladder rupture (GBR) prior to surgery in dogs undergoing cholecystectomy for treatment of gallbladder mucocele (GBM).

ANIMALS
45 dogs that underwent cholecystectomy because of GBM at a companion animal referral hospital from 2017 to 2020.

PROCEDURES
Electronic medical records were reviewed, and dogs were included if serum CRP concentration had been measured within 24 hours prior to cholecystectomy. Dogs were grouped as to whether the gallbladder was found to be ruptured or intact during surgery. Accuracy of using preoperative CRP concentration to predict GBR was compared with accuracy of abdominal ultrasonography and other preoperative blood tests.

RESULTS
GBR was present in 15 dogs at the time of surgery. Median preoperative CRP concentration was significantly higher in dogs with GBR (15.1 mg/dL; interquartile range, 7.4 to 16.8 mg/dL) than in dogs with an intact gallbladder (2.65 mg/dL; interquartile range, 0.97 to 13.4 mg/dL). Sensitivity, specificity, and accuracy of using preoperative CRP concentration to predict GBR were 100%, 67%, and 78%, respectively.

CLINICAL RELEVANCE
Measurement of preoperative CRP concentration provided excellent sensitivity and moderate specificity for detection of GBR in dogs undergoing cholecystectomy because of GBM. Accuracy of using preoperative CRP concentration for detection of GBR was not superior to the accuracy of preoperative abdominal ultrasonography. However, when CRP concentration was combined with results of ultrasonography, the sensitivity, specificity, and accuracy for detection of GBR were 100%, 93%, and 96%, respectively.
Single, large, meshed full-thickness free skin graft for reconstruction of a dorsal lumbosacral wound defect in a dog
Authors: Brown Yasmin , Cinti Filippo , Mattioli Valerio , Pisani Guido 
Published: November 2021

Abstract

CASE DESCRIPTION
A 12-year-old 32-kg neutered female crossbreed dog was treated for a large lumbosacral skin defect.

CLINICAL FINDINGS
The dog had sustained multiple, penetrating dog bite wounds to the lumbosacral and gluteal regions 5 days earlier. The referring veterinarian had initiated treatment with amoxicillin–clavulanic acid, which was continued at a dosage of 8.75 mg/kg, SC, every 24 hours at the referral hospital. Examination of the skin defect revealed a large subcutaneous abscess in the dorsal lumbosacral region with draining perimeter tracts at the wound margin. The partial-thickness wound measured 24 × 35 cm and had multifocal regions of necrosis extending caudally from the dorsal aspect of the T11 vertebra to the tail base. The skin defect was bounded by discolored and necrotic skin edges.

TREATMENT AND OUTCOME
The dog underwent extensive soft tissue wound reconstruction. A single, large, meshed full-thickness free skin graft was harvested from the left dorsolateral aspect of the thorax and grafted to the dorsal lumbosacral region, thereby enabling successful closure of the wound defect. Primary healing of the wound eventually occurred, without postoperative complications.

CLINICAL RELEVANCE
Use of a large, meshed full-thickness free skin graft led to a satisfactory outcome for this dog but required challenging postoperative management. Application of single, large, meshed full-thickness free skin grafts may be an option to manage large skin deficits in the lumbosacral area in dogs.
Development of renal adenocarcinoma in a ferret with renal cortical cysts (Mustela putorius furo)
Authors: Wong Amanda D. , Laniesse Delphine , zur Linden Alex , Singh Ameet , Susta Leonardo , Beaufrère Hugues 
Published: November 2021

Abstract

CASE DESCRIPTION
A 5.5-year-old 0.929-kg spayed female domestic ferret (Mustela putorius furo) underwent serial abdominal ultrasonographic and clinicopathologic examinations after multiple renal cysts were detected bilaterally during a routine examination.

CLINICAL FINDINGS
The ferret was apparently healthy at the start of the monitoring period and had no clinical signs for > 20 months. Four months after the initial examination, the largest cyst became increasingly mineralized; 17 months after detection, it had increased in size and become amorphous, and the ferret’s plasma BUN concentration was mildly high. Within 21 months after the first visit, a nodule was detectable, and hydronephrosis developed in the kidney with the largest cyst. Findings for fine-needle aspirates from the nodule were consistent with renal carcinoma.

TREATMENT AND OUTCOME
Contrast-enhanced CT revealed severe unilateral nephromegaly with no contrast uptake in the affected ureter. Following surgical removal of the affected kidney, histologic examination identified renal adenocarcinoma replacing the entire renal cortex and medulla. The ferret was euthanized postoperatively because of declining condition. On necropsy, metastasis to a mesenteric lymph node was identified; comorbidities included 2 other neoplasms and acute, severe injury of the contralateral kidney.

CLINICAL RELEVANCE
Neoplastic transformation of a renal cyst was suspected in the ferret of this report on the basis of observed ultrasonographic changes over time and extensive infiltration of the neoplasm throughout the affected kidney. Renal cysts are linked to renal neoplasia in other species, and the findings for this patient supported the need for periodic monitoring of renal cysts in ferrets.
MR. GEORGE HARVEY’S SHADOW ARCHETYPE AS SEEN IN ALICE SEBOLD’S THE LOVELY BONES
Authors: Dianika Rafida Arge 
Published: November 2021

The thesis writer focuses on the analysis of Mr. George Harvey’s Shadow Archetype as seen in Alice Sebold’s The Lovely Bones. The objectives of the study are, to find out the types of Mr. George Harvey’s Shadow Archetype, the causes of Mr. George Harvey’s Shadow Archetype and the effects of Mr. George Harvey’s Shadow Archetype as seen in Alice Sebold’s The Lovely Bones on himself and on neighborhood (Joe Ellis). The thesis writer applies the theory of the psyche, archetypes and shadow archetype, the causes of shadow archetype, the effects of shadow archetype and she also applies a qualitative research.Keywords: The Psyche, Archetype, Shadow
Archetype in Grimm’s Cinderella and Rapunzel
Authors: Masofa Ima 
Published: November 2021

This article attempts to analyze archetype in Grimm’s Cinderella and Rapunzel that uses comparative studies to find differences and similarities of archetype in Grimm’s Cinderella and Rapunzel. This article uses archetype concepts that two stories have collective unconsciousness that love can solve problem. This article uses comparative studies to find archetype in Grimm’s Cinderella and Rapunzel. The study aims at comparing two different literary works. The results of this article can be formulated as follows: 1) Archetype concepts can be seen in Grimm’s Cinderella and Rapunzel because they have same characters types and themes. The two main characters of two stories face complication from harsh condition but after meeting a prince, their life change better. The themes of two stories are love. 2) Archetype in Grimm’s Cinderella and Rapunzel have similarities and differences. The similarities of the two stories are the two main characters faces complication because of bad women, themes of the two stories are love. Archetype in Grimm’s Cinderella and Rapunzel get resolution by marrying a prince. The differences of two stories are the way of the two main characters to meet a prince. In Cinderella comes to the prince party with fairy’s help and leaves her shoes that lead to prince meets and marries her. In Grimm’s Rapunzel, she meets a prince in a tower by using her long hair. Keywords: Archetype, Collective unconsciousness, Comparative Studies.