bluuebunny/crossref_metadata_2025_split huggingface dataset:
Labrador Sea freshening linked to Beaufort Gyre freshwater release
Authors: Zhang Jiaxu , Weijer Wilbert , Steele Michael , Cheng Wei , Verma Tarun , Veneziani Milena 
Published: February 2021

AbstractThe Beaufort Gyre (BG), the largest Arctic Ocean freshwater reservoir, has drastically increased its liquid freshwater content by 40% in the past two decades. If released within a short period, the excess freshwater could potentially impact the large-scale ocean circulation by freshening the upper subpolar North Atlantic. Here, we track BG-sourced freshwater using passive tracers in a global ocean sea-ice model and show that this freshwater exited the Arctic mostly through the Canadian Arctic Archipelago, rather than Fram Strait, during an historical release event in 1983–1995. The Labrador Sea is the most affected region in the subpolar North Atlantic, with a freshening of 0.2 psu on the western shelves and 0.4 psu in the Labrador Current. Given that the present BG freshwater content anomaly is twice the historical analog studied here, the impact of a future rapid release on Labrador Sea salinity could be significant, easily exceeding similar fluxes from Greenland meltwater.
Percolation of heterogeneous flows uncovers the bottlenecks of infrastructure networks
Authors: Hamedmoghadam Homayoun , Jalili Mahdi , Vu Hai L. , Stone Lewi 
Published: February 2021

AbstractWhether it be the passengers’ mobility demand in transportation systems, or the consumers’ energy demand in power grids, the primary purpose of many infrastructure networks is to best serve this flow demand. In reality, the volume of flow demand fluctuates unevenly across complex networks while simultaneously being hindered by some form of congestion or overload. Nevertheless, there is little known about how the heterogeneity of flow demand influences the network flow dynamics under congestion. To explore this, we introduce a percolation-based network analysis framework underpinned by flow heterogeneity. Thereby, we theoretically identify bottleneck links with guaranteed decisive impact on how flows are passed through the network. The effectiveness of the framework is demonstrated on large-scale real transportation networks, where mitigating the congestion on a small fraction of the links identified as bottlenecks results in a significant network improvement.
A stable cathode-solid electrolyte composite for high-voltage, long-cycle-life solid-state sodium-ion batteries
Authors: Wu Erik A. , Banerjee Swastika , Tang Hanmei , Richardson Peter M. , Doux Jean-Marie , Qi Ji , Zhu Zhuoying , Grenier Antonin , Li Yixuan , Zhao Enyue , Deysher Grayson , Sebti Elias , Nguyen Han , Stephens Ryan , Verbist Guy , Chapman Karena W. , Clément Raphaële J. , Banerjee Abhik , Meng Ying Shirley , Ong Shyue Ping 
Published: February 2021

AbstractRechargeable solid-state sodium-ion batteries (SSSBs) hold great promise for safer and more energy-dense energy storage. However, the poor electrochemical stability between current sulfide-based solid electrolytes and high-voltage oxide cathodes has limited their long-term cycling performance and practicality. Here, we report the discovery of the ion conductor Na3-xY1-xZrxCl6 (NYZC) that is both electrochemically stable (up to 3.8 V vs. Na/Na+) and chemically compatible with oxide cathodes. Its high ionic conductivity of 6.6 × 10−5 S cm−1 at ambient temperature, several orders of magnitude higher than oxide coatings, is attributed to abundant Na vacancies and cooperative MCl6 rotation, resulting in an extremely low interfacial impedance. A SSSB comprising a NaCrO2 + NYZC composite cathode, Na3PS4 electrolyte, and Na-Sn anode exhibits an exceptional first-cycle Coulombic efficiency of 97.1% at room temperature and can cycle over 1000 cycles with 89.3% capacity retention at 40 °C. These findings highlight the immense potential of halides for SSSB applications.
Flavivirus maturation leads to the formation of an occupied lipid pocket in the surface glycoproteins
Authors: Renner Max , Dejnirattisai Wanwisa , Carrique Loïc , Martin Itziar Serna , Karia Dimple , Ilca Serban L. , Ho Shu F. , Kotecha Abhay , Keown Jeremy R. , Mongkolsapaya Juthathip , Screaton Gavin R. , Grimes Jonathan M. 
Published: February 2021

AbstractFlaviviruses such as Dengue (DENV) or Zika virus (ZIKV) assemble into an immature form within the endoplasmatic reticulum (ER), and are then processed by furin protease in the trans-Golgi. To better grasp maturation, we carry out cryo-EM reconstructions of immature Spondweni virus (SPOV), a human flavivirus of the same serogroup as ZIKV. By employing asymmetric localised reconstruction we push the resolution to 3.8 Å, enabling us to refine an atomic model which includes the crucial furin protease recognition site and a conserved Histidine pH-sensor. For direct comparison, we also solve structures of the mature forms of SPONV and DENV to 2.6 Å and 3.1 Å, respectively. We identify an ordered lipid that is present in only the mature forms of ZIKV, SPOV, and DENV and can bind as a consequence of rearranging amphipathic stem-helices of E during maturation. We propose a structural role for the pocket and suggest it stabilizes mature E.
A synergetic effect of BARD1 mutations on tumorigenesis
Authors: Li Wenjing , Gu Xiaoyang , Liu Chunhong , Shi Yanyan , Wang Pan , Zhang Na , Wu Rui , Leng Liang , Xie Bingteng , Song Chen , Li Mo 
Published: February 2021

AbstractTo date, a large number of mutations have been screened from breast and ovarian cancer patients. However, most of them are classified into benign or unidentified alterations due to their undetectable phenotypes. Whether and how they could cause tumors remains unknown, and this significantly limits diagnosis and therapy. Here, in a study of a family with hereditary breast and ovarian cancer, we find that two BARD1 mutations, P24S and R378S, simultaneously exist in cis in surviving cancer patients. Neither of the single mutations causes a functional change, but together they synergetically impair the DNA damage response and lead to tumors in vitro and in vivo. Thus, our report not only demonstrates that BARD1 defects account for tumorigenesis but also uncovers the potential risk of synergetic effects between the large number of cis mutations in individual genes in the human genome.
Prioritizing variants of uncertain significance for reclassification using a rule-based algorithm in inherited retinal dystrophies
Authors: Iancu Ionut-Florin , Avila-Fernandez Almudena , Arteche Ana , Trujillo-Tiebas Maria Jose , Riveiro-Alvarez Rosa , Almoguera Berta , Martin-Merida Inmaculada , Del Pozo-Valero Marta , Perea-Romero Irene , Corton Marta , Minguez Pablo , Ayuso Carmen 
Published: February 2021

AbstractInherited retinal dystrophies (IRD) are a highly heterogeneous group of rare diseases with a molecular diagnostic rate of >50%. Reclassification of variants of uncertain significance (VUS) poses a challenge for IRD diagnosis. We collected 668 IRD cases analyzed by our geneticists using two different clinical exome-sequencing tests. We identified 114 unsolved cases pending reclassification of 125 VUS and studied their genomic, functional, and laboratory-specific features, comparing them to pathogenic and likely pathogenic variants from the same cohort (N = 390). While the clinical exome used did not show differences in diagnostic rate, the more IRD-experienced geneticist reported more VUS (p = 4.07e-04). Significantly fewer VUS were reported in recessive cases (p = 2.14e-04) compared to other inheritance patterns, and of all the genes analyzed, ABCA4 and IMPG2 had the lowest and highest VUS frequencies, respectively (p = 3.89e-04, p = 6.93e-03). Moreover, few frameshift and stop-gain variants were found to be informed VUS (p = 6.73e-08 and p = 2.93e-06). Last, we applied five pathogenicity predictors and found there is a significant proof of deleteriousness when all score for pathogenicity in missense variants. Altogether, these results provided input for a set of rules that correctly reclassified ~70% of VUS as pathogenic in validation datasets. Disease- and setting-specific features influence VUS reporting. Comparison with pathogenic and likely pathogenic variants can prioritize VUS more likely to be reclassified as causal.
Inter-body coupling in electro-quasistatic human body communication: theory and analysis of security and interference properties
Authors: Nath Mayukh , Maity Shovan , Avlani Shitij , Weigand Scott , Sen Shreyas 
Published: February 2021

AbstractRadiative communication using electromagnetic fields is the backbone of today’s wirelessly connected world, which implies that the physical signals are available for malicious interceptors to snoop within a 5–10 m distance, also increasing interference and reducing channel capacity. Recently, Electro-quasistatic Human Body Communication (EQS-HBC) was demonstrated which utilizes the human body’s conductive properties to communicate without radiating the signals outside the body. Previous experiments showed that an attack with an antenna was unsuccessful at a distance more than 1 cm from the body surface and 15 cm from an EQS-HBC device. However, since this is a new communication modality, it calls for an investigation of new attack modalities—that can potentially exploit the physics utilized in EQS-HBC to break the system. In this study, we present a novel attack method for EQS-HBC devices, using the body of the attacker itself as a coupling surface and capacitive inter-body coupling between the user and the attacker. We develop theoretical understanding backed by experimental results for inter-body coupling, as a function of distance between the subjects. We utilize this newly developed understanding to design EQS-HBC transmitters that minimizes the attack distance through inter-body coupling, as well as the interference among multiple EQS-HBC users due to inter-body coupling. This understanding will allow us to develop more secure and robust EQS-HBC based body area networks in the future.
Twenty-first century droughts have not increasingly exacerbated fire season severity in the Brazilian Amazon
Authors: Libonati R. , Pereira J. M. C. , Da Camara C. C. , Peres L. F. , Oom D. , Rodrigues J. A. , Santos F. L. M. , Trigo R. M. , Gouveia C. M. P. , Machado-Silva F. , Enrich-Prast A. , Silva J. M. N. 
Published: February 2021

AbstractBiomass burning in the Brazilian Amazon is modulated by climate factors, such as droughts, and by human factors, such as deforestation, and land management activities. The increase in forest fires during drought years has led to the hypothesis that fire activity decoupled from deforestation during the twenty-first century. However, assessment of the hypothesis relied on an incorrect active fire dataset, which led to an underestimation of the decreasing trend in fire activity and to an inflated rank for year 2015 in terms of active fire counts. The recent correction of that database warrants a reassessment of the relationships between deforestation and fire. Contrasting with earlier findings, we show that the exacerbating effect of drought on fire season severity did not increase from 2003 to 2015 and that the record-breaking dry conditions of 2015 had the least impact on fire season of all twenty-first century severe droughts. Overall, our results for the same period used in the study that originated the fire-deforestation decoupling hypothesis (2003–2015) show that decoupling was clearly weaker than initially proposed. Extension of the study period up to 2019, and novel analysis of trends in fire types and fire intensity strengthened this conclusion. Therefore, the role of deforestation as a driver of fire activity in the region should not be underestimated and must be taken into account when implementing measures to protect the Amazon forest.
A surfactant polymer wound dressing protects human keratinocytes from inducible necroptosis
Authors: Khandelwal Puneet , Das Amitava , Sen Chandan K. , Srinivas Sangly P. , Roy Sashwati , Khanna Savita 
Published: February 2021

AbstractChronic wounds show necroptosis from which keratinocytes must be protected to enable appropriate wound re-epithelialization and closure. Poloxamers, a class of synthetic triblock copolymers, are known to be effective against plasma membrane damage (PMD). The purpose of this study is to evaluate the efficacy of a specific poloxamer, surfactant polymer dressing (SPD), which is currently used clinically as wound care dressing, against PMD in keratinocytes. Triton X-100 (TX100) at sub-lytic concentrations caused PMD as demonstrated by the efflux of calcein and by the influx of propidium iodide and FM1-43. TX100, an inducer of necroptosis, led to mitochondrial fragmentation, depletion of nuclear HMGB1, and activation of signaling complex associated with necroptosis (i.e., activation of RIP3 and phosphorylation of MLKL). All responses following exposure of human keratinocytes to TX100 were attenuated by pre- or co-treatment with SPD (100 mg/ml). The activation and translocation of phospho-MLKL to the plasma membrane, taken together with depletion of nuclear HMGB1, characterized the observed cell death as necroptosis. Thus, our findings show that TX100-induced plasma membrane damage and death by necroptosis were both attenuated by SPD, allowing keratinocyte survival. The significance of such protective effects of SPD on keratinocytes in wound re-epithelialization and closure warrant further studies.
No consistent startle modulation by reward
Authors: Schutte Iris , Baas Johanna M. P. , Heitland Ivo , Kenemans J. Leon 
Published: February 2021

AbstractPrevious studies have not clearly demonstrated whether motivational tendencies during reward feedback are mainly characterized by appetitive responses to a gain or mainly by aversive consequences of reward omission. In the current study this issue was addressed employing a passive head or tails game and using the startle reflex as an index of the appetitive-aversive continuum. A second aim of the current study was to use startle-reflex modulation as a means to compare the subjective value of monetary rewards of varying magnitude. Startle responses after receiving feedback that a potential reward was won or not won were compared with a baseline condition without a potential gain. Furthermore, startle responses during anticipation of no versus potential gain were compared. Consistent with previous studies, startle-reflex magnitudes were significantly potentiated when participants anticipated a reward compared to no reward, which may reflect anticipatory arousal. Specifically for the largest reward (20-cents) startle magnitudes were potentiated when a reward was at stake but not won, compared to a neutral baseline without potential gain. In contrast, startle was not inhibited relative to baseline when a reward was won. This suggests that startle modulation during feedback is better characterized in terms of potentiation when missing out on reward rather than in terms of inhibition as a result of winning. However, neither of these effects were replicated in a more targeted second experiment. The discrepancy between these experiments may be due to differences in motivation to obtain rewards or differences in task engagement. From these experiments it may be concluded that the nature of the processing of reward feedback and reward cues is very sensitive to experimental parameters and settings. These studies show how apparently modest changes in these parameters and settings may lead to quite different modulations of appetitive/aversive motivation. A future experiment may shed more light on the question whether startle-reflex modulation after feedback is indeed mainly characterized by the aversive consequences of reward omission for relatively large rewards.
High-efficiency, flexibility and lead-free X-ray shielding multilayered polymer composites: layered structure design and shielding mechanism
Authors: Li Zefeng , Zhou Wei , Zhang Xianlong , Gao Yue , Guo Shaoyun 
Published: February 2021

AbstractTo overcome the severe toxicity and blind absorption zone of conventional lead-based shielding materials for X-rays in the 70–90 keV range, the lead-free multilayered polymer composites were designed and fabricated. The effects of the direction of incidence of the X-rays and number of layers as well as layer thickness ratio of the (tungsten/ethylene-octene copolymer)/(bismuth/ethylene-octene copolymer) layered composites on their shielding efficiency were studied systematically. Compared to the traditional polymer blending, the multilayered polymer composites exhibited the improved photon attenuation. The multilayered polymer composites (layer thickness ratio was 3:7) with 6 layers had the best X-ray shielding ability. Moreover, the X-ray shielding provided by the multi-layered interfaces and the multiple complementary effect of the absorption within the multilayered structure were firstly proposed based on computer simulations. The multilayered structural design effectively weakened the probability of the X-ray penetration. Therefore, the X-ray shielding capability can be effectively enhanced through increasing number of layers and the synergistic effect of multi-layered interfaces. The experimental results of this study can serve as guidelines for the fabrication of flexibility, lead-free, lightweight and high-efficiency X-ray shielding materials.
Running barefoot leads to lower running stability compared to shod running - results from a randomized controlled study
Authors: Hollander Karsten , Hamacher Daniel , Zech Astrid 
Published: February 2021

AbstractLocal dynamic running stability is the ability of a dynamic system to compensate for small perturbations during running. While the immediate effects of footwear on running biomechanics are frequently investigated, no research has studied the long-term effects of barefoot vs. shod running on local dynamic running stability. In this randomized single-blinded controlled trial, young adults novice to barefoot running were randomly allocated to a barefoot or a cushioned footwear running group. Over an 8-week-period, both groups performed a weekly 15-min treadmill running intervention in the allocated condition at 70% of their VO2 max velocity. During each session, an inertial measurement unit on the tibia recorded kinematic data (angular velocity) which was used to determine the short-time largest Lyapunov exponents as a measure of local dynamic running stability. One hundred running gait cycles at the beginning, middle, and end of each running session were analysed using one mixed linear multilevel random intercept model. Of the 41 included participants (48.8% females), 37 completed the study (drop-out = 9.7%). Participants in the barefoot running group exhibited lower running stability than in the shod running group (p = 0.037) with no changes during the intervention period (p = 0.997). Within a single session, running stability decreased over the course of the 15-min run (p = 0.012) without differences between both groups (p = 0.060). Changing from shod to barefoot running reduces running stability not only in the acute phase but also in the longer term. While running stability is a relatively new concept, it enables further insight into the biomechanical influence of footwear.
Dynamic and history of methane seepage in the SW Barents Sea: new insights from Leirdjupet Fault Complex
Authors: Argentino Claudio , Waghorn Kate Alyse , Vadakkepuliyambatta Sunil , Polteau Stéphane , Bünz Stefan , Panieri Giuliana 
Published: February 2021

AbstractMethane emissions from Arctic continental margins are increasing due to the negative effect of global warming on ice sheet and permafrost stability, but dynamics and timescales of seafloor seepage still remain poorly constrained. Here, we examine sediment cores collected from an active seepage area located between 295 and 353 m water depth in the SW Barents Sea, at Leirdjupet Fault Complex. The geochemical composition of hydrocarbon gas in the sediment indicates a mixture of microbial and thermogenic gas, the latter being sourced from underlying Mesozoic formations. Sediment and carbonate geochemistry reveal a long history of methane emissions that started during Late Weichselian deglaciation after 14.5 cal ka BP. Methane-derived authigenic carbonates precipitated due to local gas hydrate destabilization, in turn triggered by an increasing influx of warm Atlantic water and isostatic rebound linked to the retreat of the Barents Sea Ice Sheet. This study has implications for a better understanding of the dynamic and future evolution of methane seeps in modern analogue systems in Western Antarctica, where the retreat of marine-based ice sheet induced by global warming may cause the release of large amounts of methane from hydrocarbon reservoirs and gas hydrates.
High engraftment capacity of frozen ready-to-use human fecal microbiota transplants assessed in germ-free mice
Authors: Berland Magali , Cadiou Julie , Levenez Florence , Galleron Nathalie , Quinquis Benoît , Thirion Florence , Gauthier Franck , Le Chatelier Emmanuelle , Plaza Oñate Florian , Schwintner Carole , Rabot Sylvie , Lepage Patricia , Ehrlich Dusko , Doré Joël , Juste Catherine 
Published: February 2021

AbstractThe number of indications for fecal microbiota transplantation is expected to rise, thus increasing the needs for production of readily available frozen or freeze-dried transplants. Using shotgun metagenomics, we investigated the capacity of two novel human fecal microbiota transplants prepared in maltodextrin-trehalose solutions (abbreviated MD and TR for maltodextrin:trehalose, 3:1, w/w, and trehalose:maltodextrin 3:1, w/w, respectively), to colonize a germ-free born mouse model. Gavage with frozen-thawed MD or TR suspensions gave the taxonomic profiles of mouse feces that best resembled those obtained with the fresh inoculum (Spearman correlations based on relative abundances of metagenomic species around 0.80 and 0.75 for MD and TR respectively), while engraftment capacity of defrosted NaCl transplants most diverged (Spearman correlations around 0.63). Engraftment of members of the family Lachnospiraceae and Ruminoccocaceae was the most challenging in all groups of mice, being improved with MD and TR transplants compared to NaCl, but still lower than with the fresh preparation. Improvement of engraftment of this important group in maintaining health represents a challenge that could benefit from further research on fecal microbiota transplant manufacturing.
Generation of reconstituted hemato-lymphoid murine embryos by placental transplantation into embryos lacking HSCs
Authors: Jeon Hyojung , Asano Keigo , Wakimoto Arata , Kulathunga Kaushalya , Tran Mai Thi Nhu , Nakamura Megumi , Yokomizo Tomomasa , Hamada Michito , Takahashi Satoru 
Published: February 2021

AbstractIn order to increase the contribution of donor HSC cells, irradiation and DNA alkylating agents have been commonly used as experimental methods to eliminate HSCs for adult mice. But a technique of HSC deletion for mouse embryo for increase contribution of donor cells has not been published. Here, we established for the first time a procedure for placental HSC transplantation into E11.5 Runx1-deficient mice mated with G1-HRD-Runx1 transgenic mice (Runx1-/-::Tg mice) that have no HSCs in the fetal liver. Following the transplantation of fetal liver cells from mice (allogeneic) or rats (xenogeneic), high donor cell chimerism was observed in Runx1-/-::Tg embryos. Furthermore, chimerism analysis and colony assay data showed that donor fetal liver hematopoietic cells contributed to both white blood cells and red blood cells. Moreover, secondary transplantation into adult recipient mice indicated that the HSCs in rescued Runx1-/-::Tg embryos had normal abilities. These results suggest that mice lacking fetal liver HSCs are a powerful tool for hematopoiesis reconstruction during the embryonic stage and can potentially be used in basic research on HSCs or xenograft models.