bluuebunny/crossref_metadata_2025_split huggingface dataset:
Results of Surveillance of Influenza in Out-patients Influenza-like Illness (ILI), Inpatients Severe Acute Respiratory Illness (SARI) and Severe Pneumonia cases in Community Based Medical College Hospital, Bangladesh, Mymensingh during the period of May 2
Authors: Islam Md Aminul , Khan M Karim , Bhuiyan Mohammod Kamruj Jaman , Beg Ayesha , Md Asaduzzaman - , Md Badruzzaman - 
Published: June 2021

Influenza is prevalent in Bangladesh among both adults and children. This observation is proved by recent population-based estimates. A hospital based surveillance study was conducted under the supervision of ICDDRB at Community Based Medical College Hospital, Bangladesh (CBMCH,B). To explore the epidemiology and seasonality of influenza throughout the Mymensingh region and all age groups, we analyzed the data collected during the period of May 2007 to May 2012 for a period of 5 years. The result revealed that out of 1014 patients, 140(14%) were influenza positive by real time RT-PCR. Among the sample-positive patients, 90(64%) were type A and 50(36%) type B. Hemagglutinin subtyping of type A virus detected 7(8%) A/H1 and 42(47%) A/H3, 41(46%) H1N1 pdm09 but no A/H5 or other novel influenza strains. The frequency of influenza cases was highest among children aged under 5 year 44%, while the laboratory confirmed cases were highest among patients aged under 1 year to 30 years 54%. We identified a distinct influenza peak during the rainy season (May to September), highest in July and August. There is a very low presence of influenza in the month of October, November and December (2, 1, 2 flu positive cases in 5 year period respectively). Our surveillance data confirms that influenza is prevalent throughout Mymensingh, affecting a wide range of ages and causing considerable morbidity and hospital care. A unimodal influenza seasonality may allow Mymensingh, Bangladesh to time annual influenza prevention messages and vaccination campaigns to reduce the national influenza burden. To scale-up such national interventions, we need to quantify the national rates of influenza and the economic burden associated this disease through further studies.
CBMJ 2015 January: Vol. 04 No. 01 P: 03-12
Outcome of Antegrade Interlocking Intramedullary Nailing in Fracture Shaft of the Femur in Community Based Medical College Hospital, Bangladesh
Authors: Chowdhury Mamunur Rashid , Haque Md Anwarul , Uddin Md Nasir , Islam Md Saiful , Mumu Sabrina Alam , Hasan Md Sabbir , Md Touhiduzzaman - 
Published: June 2021

Day by day road traffic accident in Bangladesh is increasing. Mymensingh-Dhaka highway passes beside the Community Based Medical College Hospital, Bangladesh. We often have to manage a greater number of traumatic patients. In road traffic accident the incidence of fracture shaft of femur is the most common. That's why my study was done to evaluate the result of management of fracture shaft of the femur with antegrade interlocking intramedullary nailing under the department of Orthopedic Surgery in CBMCH,B in the period between January 2009 to July 2013. Total 88 patients were selected for interlocking nailing initially in the study. Out of them 8 patients did not report in subsequent follow up. So the result of the study was based on 80 patients (48 males; 32 females) with the range of 20- 68 years of age. The average age of the patient was 36 years. Motor vehicle accidents was the most common cause of fracture shaft femur (65%) and the second most common cause was fall from height (20%) specially fall from tree. Among the patients; right sided femur involvement was 67.5% (54) and left sided femur involvement was 32.5% (26). The majority fracture of the shaft of the femur was lower third (70%). The patients were mostly in the day-laborers' groups (35%), and, the next group was students. The mean union time was 18 weeks with the range of 15-30 weeks. After operation, complications were found such as; infection 3.75%(3), non-union 1.25% (1). Excellent functional outcome was found in 82.5% (66), good in 12.5% (10), fair in 5%(4). This functional outcome study was evaluated by Klemm & Borner 1 criteria.
CBMJ 2015 January: Vol. 04 No. 01 P: 13-17
A Case of Congenitally Corrected Transposition of Great Arteries (CCTGA)
Authors: Ahmed Tofayel Uddin , Rahman M Mafizur , Khan Parvez Rahman , Hasan Mohammad Rajibul 
Published: June 2021

Recent years, much scientific attention has been given to congenital heart diseases (CHD) and probable complications. Congenitally corrected transposition of the great arteries (CCTGA) is a rare, complex form of congenital heart defects. CCTGA is characterized by atrioventricular (AV) and ventriculoarterial (VA) discordance and hence by a physiologically normal direction of blood flow sometimes called "double discordance". The development of complete AV block and global ventricular dysfunction has been identified as the cause of cardiac death. This paper presents a case of CCTGA with rhythm disorders and exertional dyspnea.
CBMJ 2015 January: Vol. 04 No. 01 P: 37-40
Assessment of status and threats of wild mammals in some selected locations of Dinajpur district, Bangladesh
Authors: Haque Md Amdadul , Khan M Monirul H , Kabir Md Mofizul 
Published: June 2021

The status of mammals in Dinajpur district was studied from October 2016 to November 2017. For inventory and assessment of wild mammal’s belt-transect sampling, box trap survey, mist netting, night survey and questionnaire survey methods were used. A total of 22 species of mammals belonging to 11 families and 5 orders were recorded. Of the recorded species 8 were rodents, 1 hare, 1 shrew, 7 bats and 5 carnivores. Muridae appeared as the largest family containing 8 species. A total of 9 species (41%) were recorded as “very common”, 6 species (27%) “common”, 4 species (18%) “uncommon”, and 3 species (14%) “rare”. Among all the mammalian species, 41% (9 species) were nocturnal, 14% (3 species) crepuscular, 37% (8 species) metaturnal and 9% (2 species) diurnal. On the other hand, 55% (12 species) of mammals were terrestrial, 9% (2 species) arboreal, 32% (7 species) aerial and 4% (1 species) aquatic mammals. Of all the recorded species, 32% (7 species) were herbivores followed by 27% (6 species) carnivores, 5% (1 species) opportunists while frugivores and insectivores were 18% (4 species) each. About 19% (4 species) of the known species are nationally threatened comprising of Endangered (5%), Vulnerable (5%) and Near Threatened (9%) and Least Concern (81%). Based on the habitats, 14 species (64%) were recorded from forests, 7 (31%) from homestead vegetation’s and 1 (5%) species from wetlands. A good number of individuals (n=12) of the nationally threatened Bengal Fox (Vulpes bengalensis) were found in the study area and two mammal species, Indian Hare (Lepus nigricollis) and Large Indian Civet (Viverra zibetha) have been locally facing the threat of extirpation. The mammalian diversity in the natural forests (64%) was higher than that of the other habitats. Different anthropogenic threats, especially hunting by ethnic Santal people (frequency of observation 24 times, impact point 4), exist in the study area. The major proposed actions for the study area are habitat restoration, increase public awareness and conflict management.
Jahangirnagar University J. Biol. Sci. 9(1 & 2): 21-34, 2020 (June & December)
Comparative karyomorphological analysis of two varieties of Brassica campestris from Bangladesh
Authors: Warasy Ashma Ahmed 
Published: June 2021

Two varieties of the oilseed plant viz. Brassica campestris var. Tori-7 and Brassica campestris var. BARI Sarisha-9 were karyomorphologically investigated following orcein staining. Presence of “Round Pro-chromosome Type” of interphase nuclei and “Continuous Type” of prophase chromosomes indicating that both Tori-7 and BARI Sarisha-9 were originated from common ancestor. Moreover, both the varieties were found to possess 2n=20 chromosomes with almost similar range of length i.e.; 0.90±0.04-2.51±0.05μm for Tori-7 and 0.99±0.06-2.60±0.06μm for BARI Sharisha-9. The total length of 2n chromosome complement was recorded as 29.61±0.76μm for Tori-7 and 29.75±0.80μm for BARI Sharisha- 9 where a slight gradual decrease of chromosomal length was observed in both cases. Average chromosomal length, range of individual chromosomal length, relative length and centromeric index of both varieties were more or less similar. Karyotype symmetry index and Karyotype asymmetry index indicating the symmetric nature of both varieties. All metacentric chromosomes were found in Tori-7 representing strictly symmetric karyotype. Beside metacentric, 2 sub-metacentric chromosomes were found in BARI Sharisha-9 representing almost symmetric karyotype. Thus the two varieties of Brassica campestris -were plants of primitive nature. However, BARI Sharisha-9 can be considered comparatively advanced than Tori-7 based on different cytogenetical features. Therefore, the compilation of these karyomorphological information will be useful for authentic identification and characterization of the two varieties of Brassica campestris which are very basic and important information for breeding in crop improvement programme.
Jahangirnagar University J. Biol. Sci. 9(1 & 2): 69-77, 2020 (June & December)
Assessment of molecular and clinical characteristics in blood transfusion dependent HbE/β-thalassemia patients
Authors: Islam Nazneen Naher , Hossain Mohabbat , Das Shuvo Chandra , Seuti Sabrina Alam , Chowdhury Mahmood A 
Published: June 2021

Hb E/β-thalassemia is one of the most common forms of hemoglobinopathies worldwide. This life-threatening genetic disorder is not completely curable. This study aims to investigate the correlation of mutations with clinical manifestations among the transfusion dependent Hb E/β- thalassemia patients using molecular, hematological and biochemical methods. A total of 60 blood samples were collected from regular blood transfusion-dependent HbE/β-thalassemia patients. Mutations within HBB gene were analysed by Sanger sequencing, BLAST 2.0, HbVar database and Alibaba 2.1. Clinical data were analysed based on CBC, Hemoglobin Electrophoresis and Serum Ferritin assay. Results demonstrate that a total of three mutationsdeletion of A (+23bp in HBB promoter region), c.9 T>C and c.79 G>A were detected among the study population, where deletion of A (+23bp in HBB promoter region) is a novel mutation. A total of three (USF, AP-1 and GATA-1) important putative binding sites were identified within the mutated region of HBB gene. Hematological analysis also showed remarkable correlation between the mutations and clinical manifestations in the patients. Reduced levels of RBC, Hb, MCV and MCH were found among the patients than the normal. Highly increased level of serum ferritin was also found among the patients due to the frequent destruction of RBCs. In conclusion, the findings of this study will be helpful to understand the mutational and hematological status of HbE/β-thalassemia patients. And this study will also be beneficial for effective molecular drug designing, drug response and other therapeutic approach for HbE/β-thalassemia patients.
Jahangirnagar University J. Biol. Sci. 9(1 & 2): 109-121, 2020 (June & December)
Avaliação do desempenho produtivo e especialização da laranja (Citrus sinensis L. Osbeck) no Pará no período de 2008 a 2018 utilizando métodos de análise de desenvolvimento regional associados a geotecnologias
Authors: Rosário Raimara Reis do , David Érica Coutinho , Pereira André Gustavo Campinas , Viana Josiene Amanda dos Santos , Tavares Treyce Stephane Cristo , Santos Junior Leonardo Nascimento dos , Oliveira Suelen Melo de , Souza Lourivan Carneiro de , Barros Márcia Nazaré Rodrigues , Silva Junior Osmar Guedes da 
Published: June 2021

O objetivo do estudo foi caracterizar a dinâmica produtiva e a especialização da citricultura nas 22 microrregiões paraenses no período de 2008 a 2018, utilizando como ferramentas de análise as geotecnologias e técnicas de análise regional. Para realizar a análise, utilizou-se a base de dados de produção agrícola disponibilizada pela plataforma do Sistema IBGE de Recuperação Automática (SIDRA), com o auxílio do software Microsoft Excel®, foram posteriormente tabulados em planilhas, para o tratamento por meio de ferramentas estatísticas ofertadas pelo programa, o qual, visou a análise do Quociente Locacional (QL) e manipulação de tabelas para elaboração dos mapas coropléticos, que foram executados utilizando o Software ArcGis 10.5. A partir da análise foi possível identificar que entre o intervalo de 2008-2018, a microrregião do Guamá foi a única região a apresentar os maiores índices QL em todo o período avaliado, apresentando um crescimento de 85,47% para este indicador. Com relação ao quantitativo de produção, as maiores microrregiões paraenses produtoras de Laranjas, foram: Guamá, Santarém, Itaituba, Bragantina, Altamira e Tomé-Açu, com médias anuais a 169.890,4 t, 12.195,3 t, 4.305,8 t, 3.753,9 t, 3,304,7 t e 2.967,9 t, respectivamente. Desta forma, foi possível compreender que uma região especializada pode não apresentar uma grande produtividade de laranja, assim como, uma região não especializada pode vir a ser uma grande produtora do fruto. Além disso, pode ocorrer de uma região especializada conseguir apresentar uma grande produção agrícola total.
Left Ventricular Remodeling Following Balloon Mitral Valvuloplasty in Rheumatic Mitral Stenosis: Magnetic Resonance Imaging Study
Authors: Samaan Amir Anwar , Said Karim , Aroussy Wafaa El , Hassan Mohammed , Romeih Soha , El Sawy Amr , Fawzy Mohammed Eid , Yacoub Magdi 
Published: June 2021

Background: Rheumatic heart disease affects primarily cardiac valves, it could involve the myocardium either primarily or secondary to heart valve affection. The influence of balloon mitral valvuloplasty (BMV) on left ventricular function has not been sufficiently studied.Aim: To determine the influence of balloon mitral valvuloplasty (BMV) on both global and regional left ventricular (LV) function.Methods: Thirty patients with isolated rheumatic mitral stenosis (MS) were studied. All patients had cardiac magnetic resonance imaging (CMR) before, 6 months and 1 year after successful BMV. LV volumes, ejection fraction (EF), regional and global LV deformation, and LV late gadolinium enhancement were evaluated.Results: At baseline, patients had median EF of 57 (range: 45–69) %, LVEDVI of 74 (44–111) ml/m2 and LVESVI of 31 (14–57) ml/m2 with absence of late gadolinium enhancement in all myocardial segments. Six months following BMV, there was a significant increase in LV peak systolic global longitudinal strain (GLS) (−16.4 vs. −13.8, p &lt; 0.001) and global circumferential strain (GCS) (−17.8 vs. −15.6, p = 0.002). At 1 year, there was a trend towards decrease in LVESVI (29 ml/m2, p = 0.079) with a significant increase in LV EF (62%, p &lt; 0.001). A further significant increase, compared to 6 months follow up studies, was noticed in GLS (−17.9 vs. −16.4, p = 0.008) and GCS (−19.4 vs. −17.8 p = 0.03).Conclusions: Successful BMV is associated with improvement in global and regional LV systolic strain which continues for up to 1 year after the procedure.
A Coronavirus Outbreak Linked to a Funeral Among a Romani Community in Central Italy
Authors: Ripabelli Giancarlo , Sammarco Michela Lucia , Cannizzaro Fabio , Montanaro Carmen , Ponzio Guido Vincenzo , Tamburro Manuela 
Published: June 2021

Background: The epidemic dynamics of COVID-19 in the Molise region, central Italy, has dramatically changed from the beginning of May 2020, which was when infections were reported amongst Romani people. The aims of this study were to describe the characteristics of an outbreak that occurred in the Romani community and the interventions implemented for control.Methods: A retrospective analysis of outbreak data was performed to describe the SARS-CoV-2 transmission dynamics.Results: A young Romani woman was the first case reported and epidemiological investigation established a possible link with the funeral of a deceased member of this community. In total, 150 close contacts within 34 family groups in two cities were traced, and 109 (72.7%) Romani individuals were found to be infected by COVID-19. The patient's median age was 31 years, 58% were female, and the highest (20.2%) incidence occurred in the 0–9 years age group. A total of 26 (23.8%) patients developed typical SARS-CoV-2 symptoms, 15 (57.8%) were hospitalized, and 21 (22.1%) had comorbidities [most commonly hypertension (28.6%) and/or coronary heart diseases (23.8%)]. The outbreak was effectively controlled through compulsory quarantine and enhanced active surveillance.Conclusions: This is the first study providing insight into COVID-19 transmission dynamics among a Romani population living in Italy. These findings support general conclusions about the role of crowded social gatherings in SARS-CoV-2 spread, the high communicability among close contacts and household settings, and the impact of asymptomatic carriers. These features are of relevance to certain Romani customs where family gatherings are a fundamental pillar of their lives. Although difficulties emerged in interacting with Romani people related to cultural drivers, beliefs, and lifestyle, the outbreak management was effective and should be considered as a valuable model applicable to similar incidents occurring in minority populations.
Extended Ganglion Cell Layer Thickness Deviation Maps With OCT in Glaucoma Diagnosis
Authors: Lehmann Paul , Hohberger Bettina , Lämmer Robert , Mardin Christian 
Published: June 2021

Purpose: The aim of the present study was to investigate the diagnostic power of RGCL in the macula quantitatively and qualitatively by using a conventional and extended elliptic grid with deviation maps.Subjects and Methods: Thickness of RGCL was measured using SPECTRALIS® OCT (Heidelberg Engineering, Heidelberg, Germany) in 150 eyes of 150 subjects of the Erlangen Glaucoma Registry (EGR; NTC00494923): 26 ocular hypertension (OHT), 39 pre-perimetric open-angle glaucoma (pre-OAG), 19 normal tension glaucoma (NTG), 34 primary open-angle glaucoma (POAG), 16 secondary open-angle glaucoma (SOAG), and 16 controls. Analysis of RGCL was done quantitatively (global value, GV) and qualitatively (qualitative total value, QTV) by using a color-coded point score for data of the common elliptic macular grid of deviation maps. Furthermore, qualitative analysis of RGCL was done for an extended elliptic macula grid (extended qualitative total value, eQTV). Receiver operating characteristic (ROC) curves were calculated for the conventional and the enlarged macular grid for all subjects' groups.Results: GV of RGCL thickness differed significantly between pre-OAG (p &lt; 0.05), NTG (p &lt; 0.001), POAG (p &lt; 0.001), SOAG (p &lt; 0.001), yet not OHT (p &gt; 0.05) and controls, respectively. Quantitative ROC analysis of GV showed AUC of 0.965 (SOAG), 0.942 (POAG), 0.916 (NTG), 0.772 (pre-OAG), and 0.526 (OHT). QTV differed significantly between pre-POAG (p &lt; 0.05), NTG (p &lt; 0.001), POAG (p &lt; 0.001), SOAG (p &lt; 0.001), yet not OHT (p &gt; 0.05) and controls, respectively. Qualitative ROC analysis of QTV showed AUCs of 0.908 (NTG) 0.914 (POAG), 0.930 (SOAG), 0.734 (pre-POAG), and 0.519 (OHT). Implementation of eQTV yielded even higher AUCs for NTG (0.919), POAG (0.969), and SOAG (0.973) compared to GV. Similar AUCs of eQTV and GV were observed for OHT (0.514) and pre-OAG (0.770).Conclusion: The results of the present study showed that quantitative and qualitative analysis of RGCL thickness yielded similar diagnostic impacts compared to RNFL. Qualitative analysis might be a quick and easy useable tool for clinical all-day life. The present data suggest that analysis of an extended macula region might improve its diagnostic impact.
Differential microRNA Expression in Newcastle Disease Virus-Infected HeLa Cells and Its Role in Regulating Virus Replication
Authors: Chen Yu , Zhu Shanshan , Pei Yuru , Hu Jiao , Hu Zenglei , Liu Xiaowen , Wang Xiaoquan , Gu Min , Hu Shunlin , Liu Xiufan 
Published: June 2021

As an oncolytic virus, Newcastle disease virus (NDV) can specifically kill tumor cells and has been tested as an attractive oncolytic agent for cancer virotherapy. Virus infection can trigger the changes of the cellular microRNA (miRNA) expression profile, which can greatly influence viral replication and pathogenesis. However, the interplay between NDV replication and cellular miRNA expression in tumor cells is still largely unknown. In the present study, we compared the profiles of cellular miRNAs in uninfected and NDV-infected HeLa cells by small RNA deep sequencing. Here we report that NDV infection in HeLa cells significantly changed the levels of 40 miRNAs at 6 h post-infection (hpi) and 62 miRNAs at 12 hpi. Among 23 highly differentially expressed miRNAs, NDV infection greatly promoted the levels of 3 miRNAs and suppressed the levels of 20 miRNAs at both time points. These 23 miRNAs are predicted to target various genes involved in virus replication and antiviral immunity such as ErbB, Jak-STAT, NF-kB and RIG-I-like receptor. Verification of deep sequencing results by quantitative RT-PCR showed that 9 out of 10 randomly selected miRNAs chosen from this 23-miRNA pool were consistent with deep sequencing data, including 6 down-regulated and 3 up-regulated. Further functional research revealed that hsa-miR-4521, a constituent in this 23-miRNA pool, inhibited NDV replication in HeLa cells. Moreover, dual-luciferase and gene expression array uncovered that the member A of family with sequence similarity 129 (FAM129A) was directly targeted by hsa-miR-4521 and positively regulated NDV replication in HeLa cells, indicating that hsa-miR-4521 may regulate NDV replication via interaction with FAM129A. To our knowledge, this is the first report of the dynamic cellular miRNA expression profile in tumor cells after NDV infection and may provide a valuable basis for further investigation on the roles of miRNAs in NDV-mediated oncolysis.
Freely Generated Word Responses Analyzed With Artificial Intelligence Predict Self-Reported Symptoms of Depression, Anxiety, and Worry
Authors: Kjell Katarina , Johnsson Per , Sikström Sverker 
Published: June 2021

BackgroundQuestion-based computational language assessments (QCLA) of mental health, based on self-reported and freely generated word responses and analyzed with artificial intelligence, is a potential complement to rating scales for identifying mental health issues. This study aimed to examine to what extent this method captures items related to the primary and secondary symptoms associated with Major Depressive Disorder (MDD) and Generalized Anxiety Disorder (GAD) described in the Diagnostic and Statistical Manual of Mental Disorders (DSM-5). We investigated whether the word responses that participants generated contained information of all, or some, of the criteria that define MDD and GAD using symptom-based rating scales that are commonly used in clinical research and practices.MethodParticipants (N = 411) described their mental health with freely generated words and rating scales relating to depression and worry/anxiety. Word responses were quantified and analyzed using natural language processing and machine learning.ResultsThe QCLA correlated significantly with the individual items connected to the DSM-5 diagnostic criteria of MDD (PHQ-9; Pearson’s r = 0.30–0.60, p &lt; 0.001) and GAD (GAD-7; Pearson’s r = 0.41–0.52, p &lt; 0.001; PSWQ-8; Spearman’s r = 0.52–0.63, p &lt; 0.001) for respective rating scales. Items measuring primary criteria (cognitive and emotional aspects) yielded higher predictability than secondary criteria (behavioral aspects).ConclusionTogether these results suggest that QCLA may be able to complement rating scales in measuring mental health in clinical settings. The approach carries the potential to personalize assessments and contributes to the ongoing discussion regarding the diagnostic heterogeneity of depression.