bluuebunny/crossref_metadata_2025_split huggingface dataset:
Metabolomic Analysis and Identification of Sperm Freezability Biomarkers in Boar Seminal Plasma
Authors: Zhang Yu-ting , Liang Han-lin , Liu Yan , Zhao Meng , Xu Qian-qian , Liu Zhong-hua , Weng Xiao-gang 
Published: April 2021

Abstract
Background: In the freezing process of boar sperm, there are obvious differences of freezability between individuals. Studies suggest that specific freezability markers might be useful in good (GFE) and poor freezabitity ejaculates (PFE) selection prior to cryopreservation. Some potential markers of boar sperm freezability have been found from spermatozoa, little attention has been paid to seminal plasma. The seminal plasma is composed of secretions from testis, epididymis, and accessory sex glands, and the exposure of spermatozoa to small molecules such as metabolites can affect the sperm functions. However, details and significance of the seminal plasma metabolome related to boar sperm freezability are unknown. Therefore, the main aim of this study was to explore the difference in the metabolic level of seminal plasma between boars with differential freezability, and to explore the biomarkers of semen freezing tolerance. Results: A total of 953 metabolites were identified in boar semen plasma by UHPLC-qTOF-MS analysis, and 50 metabolites show significant change between GFE group and PFE group. Further, twelve metabolites were subjected to metabolic target analysis and three metabolites (D-Aspartic acid, N-Acetyl-L-glutamate (NAG), and Inosine) show differences.Conclusions: There is significant differece on metabolome of seminal plasma between GFE and PFE individuals. The D-Aspartic acid, NAG, and Inosine in seminal plasma may be potential markers for assessing sperm cryopreservation resistance in boars.
Flood disturbance affects morphology and reproduction of woody riparian plants
Authors: Fischer Sarah , Greet Joe , Walsh Christopher , Catford Jane 
Published: April 2021

Abstract
Riparian forests are structured and maintained by their hydrology. Woody riparian plants typically adapt to the local flood regime to maximise their likelihood of survival and reproductive success. Understanding how extant trees form and reproduce in response to flood disturbance is crucial for predicting vegetation changes and informing restoration. Working in a temperate evergreen riparian forest, we aim to determine whether disturbance-based responses of plants found in other ecosystems also typify woody plants in riparian forests where disturbances are non-lethal, annual events. Using plant surveys and 20-year modelled hydrological data, we examined whether i) the morphology (main stem diameter, height, crown width, crown extent, stem leaning) and ii) reproduction type (sexual and asexual reproduction) and extent of three dominant woody species (Eucalyptus camphora, Leptospermum lanigerum and Melaleuca squarrosa) vary with flood regime (flood frequency and flood duration); and iii) whether different morphology is associated with different reproductive strategies. Increased flooding generally resulted in increased stem numbers and greater stem leaning – morphologies associated with asexual reproduction – of our study species. More frequent flooding also reduced plant size and sexual reproduction in E. camphora. Sexual reproduction in the studied species was more common in taller plants with single, more upright stems in good condition. Flexible morphology and plastic reproductive strategy may constitute an adaptation to mild or chronic disturbance in floodplains. Our findings suggest that woody plants respond to physical disturbance in consistent ways regardless of the nature of the disturbance – be it fires, hurricanes or floods.
Repeated Neonatal Sevoflurane Induced Neurocognitive Impairment Through NF-κB-Mediated Pyroptosis
Authors: Dai Jing , Li Xue , Wang Cai , Gu Shuxin , Dai Lei , Zhang Jingyun , Fan Yunxia , Wu Jing 
Published: April 2021

Abstract
Background: Exposure to general anesthesia (GA) during the postnatal period is associated with neuroinflammation and long-term neurocognitive impairment in preclinical and clinical settings. Pyroptosis, a novel type of programmed cell death that along with inflammation, plays an important role in the mechanism of diverse neurological diseases. Nevertheless, its role in GA-induced neuroinflammation and neurocognitive impairment in developing brain has not been investigated. Methods: Rats at postnatal day 6 or primary hippocampal neurons at 9 days in vitro, received 3% sevoflurane for 2 hours daily for three consecutive days. A pharmacological inhibitor of nuclear factor (NF)‑κB (BAY 11-7082) was administered to suppress NF-κB activation. Histological and biochemical analysis were performed to assess the pyroptosis and neuronal and synaptic damages both in vivo and in vitro. In addition, behavioral tests were performed to evaluate the neurocognitive ability in rats.Results: Repeated sevoflurane exposures activated NF-κB-mediated pyroptosis and neuroinflammation in the hippocampus of developing rats, caused damages in neuronal morphology and synaptic integrity, and induced neurocognitive impairment in rats. BAY 11-7082, the inhibitor of NF-κB, suppressed the activation of pyroptosis, attenuated the neuronal and synaptic damages, and ameliorated the neurocognitive impairment induced by repeated sevoflurane in the developing rats.Conclusions: These results demonstrated that repeated sevoflurane GA might induce neuroinflammation and cognitive impairment in developing rats via activation of NF-κB-mediated pyroptosis. Our findings characterize a novel role for pyroptosis as a potential therapeutic target in neuroinflammation to repeated neonatal GA.
Autopsy and premortem diagnostic discrepancy review in an Irish tertiary PICU.
Authors: O'Rahelly Mark , McDermott Michael , Healy Martina 
Published: April 2021

Abstract
Objective: 1) Review ante- and post-mortem diagnoses and assign a Goldman error classification. 2) Establish autopsy rates. Design: A retrospective analysis of autopsies performed on patients who died in Paediatric intensive care unit (PICU) between November 13th 2012 and October 31st 2018. We reviewed medical and autopsy data of all patients and Goldman classification of discrepancy between ante- and post-mortem diagnoses was assigned. Setting: Tertiary PICU. Patients: All patients that died in PICU within the designated timeframe. Interventions: Goldman error classification assignment. Measurements and main results: 396 deaths occurred in PICU from 8,329 (4.75%) admissions. 99 (25%) had an autopsy, 75 required by the coroner. All were included in the study. Fifty-three were male and 46 females. Fifty-three patients were transfers from external hospitals, 46 from our centre. Forty-one were neonates, 32 were <1 year of age, and 26 were >1 year of age. Median length of stay was 3 days. Eighteen were post cardiac surgery, and three post cardiac catheter procedure. Major diagnostic errors (Class I/II) were identified in 14 (14.1%), 2 (2%) Class I, and 12 (12.1%) were Class II errors. Class III and IV errors occurred in 28 (28.2%) patients. Complete concordance (Class V) occurred in 57 (57.5%) cases. Conclusion: The autopsy rate and the diagnostic discrepancy rate within our PICU is comparable to those previously reported. Our findings show the continuing value of autopsy in determining cause of death and providing greater diagnostic clarity. Given their value, post-mortem examinations, where indicated, should be considered part of a physician’s duty of care to families and future patients.
TTK Predicts Triple Positive Breast Cancer Prognosis and Regulates Tumor Proliferation and Invasion
Authors: Gao Yunhe , Qu Shanshan , Cao Lanqing , Yao Min 
Published: April 2021

Abstract
Background: This study was conducted to investigate the expression of the spindle assembly checkpoint kinase tyrosine/threonine kinase (TTK) in triple positive breast cancer (TPBC) and its effect on TPBC cells. Methods: We analyzed the status of TTK in 69 TPBC samples using immunohistochemistry. The correlation between TTK and clinicopathological parameters was analyzed using a chi-squared test. The prognostic value of TTK was evaluated using Kaplan–Meier survival curves. We analyzed the role of TTK in the invasion and proliferation of TPBC cells in vitro and in vivo. Results: The mean age of the 69 patients with TPBC enrolled in this study was 53 years (range: 29–86 years). TTK expression was positively correlated with tumor size (P = 0.034), p53 status (P = 0.023), TNM stage (P = 0.023), and Ki-67 index (P< 0.001). The Kaplan–Meier curves revealed that TTK expression was correlated with poor disease-free survival (P = 0.001) and overall survival (P = 0.050). Multivariate proportional hazard regression analyses showed that TTK and TNM staging were significant independent predictors of disease-free survival (P = 0.007 and P = 0.034, respectively). Additionally, TTK knockdown inhibited the invasion and proliferation of the BT474 TPBC cell line. Conclusions: The findings of the study indicate that TTK overexpression is associated with cancer progression and prognosis in patients with TPBC, whereas TTK knockdown inhibits the invasion and proliferation of TPBC cells. Thus, TTK may serve as a prognostic marker for TPBC.
Risk analysis and influencing factors of drought and flood disasters in China
Authors: Pei Wei , Tian Cuizhu , Fu Qiang , Ren Yongtai , Li Tianxiao 
Published: April 2021

Abstract
The risk analysis of flood and drought disasters and the study of their influencing factors enhance our understanding of the temporal and spatial variation law of disasters and help identify the main factors affecting disasters. This paper uses the provincial administrative region of China as the research area. The statistical distribution of flood and drought disasters was optimized from 10 alternative distributions by the KS test, and the disaster risk was analyzed. Thirty-five indicators were selected from nature, agriculture and the social economy as alternative factors. The main factors affecting flood and drought disasters were selected by Pearson, Spearman and Kendall correlation coefficient test. The results demonstrated that the distribution of floods and drought is right skewed, and the beta distribution is the best statistical distribution for fitting disasters. In terms of time, the risk of flood and drought disasters in all regions showed a downward trend. Economic development and the enhancement of the ability to resist disasters were the main reasons for the change in disasters. Spatially, the areas with high drought risk were mainly distributed in Northeast and North China, and the areas with high flood risk were mainly distributed in the south, especially in Hubei, Hunan, Jiangxi and Anhui. The distribution of floods and drought disasters was consistent with the distribution characteristics of precipitation and water resources in China. Among the natural factors, precipitation was the main factor causing changes in floods and drought disasters. Among the agricultural and socioeconomic factors, the indicators reflecting the disaster resistance ability and regional economic development level were closely related to flood and drought disasters. The research results have reference significance for disaster classification, disaster formation mechanisms and flood and drought resistance.
Social and Environmental Risk Factors for Unintentional Suffocation Among Infants in China
Authors: Yu Xue , Kang Leni , Miao Lei , Zhu Jun , Liang Juan , Dai Li , Xiaohong Li , Li Qi , Rao Rui , Wang Yanping , Yuan Chunhua , He Chunhua 
Published: April 2021

Abstract
Background: This retrospective study aims to determine the epidemiological features of deaths caused by unintentional suffocation among infants in China, with a focus on environmental and sociodemographic factors may contribute to these deaths.Methods: Data were obtained from China’s under 5 Child Mortality Surveillance System (U5CMSS) from October 1, 2015 to September 30, 2016. A total of 377 children under 1 year of age who died from unintentional suffocation were included in the survey. Primary caregivers were interviewed individually using the Unintentional Suffocation Mortality among Children under 5 Questionnaire. EpiData was used to establish the database, and the results were analysed using SPSS 22.0.Results: Most (85.9%) unintentional infant suffocations occurred in rural areas, and 67.5% occurred in infants 0 to 3 months old. Among the primary caregivers of the infants, most (82.7%) had a junior middle school education or below, and 83.1% of them lacked unintentional suffocation first aid skills. Of the 377 unintentional suffocated-infant deaths, the causes of death were unintentional suffocation and strangulation in bed (ASSB) (193, 51.2%), inhalation suffocation (154, 40.8%), other unintentional suffocation (6, 1.6%), and unknown (24, 6.4%). Among the infant deaths due to ASSB, overlaying (88.6%) was the frequently reported circumstance. A total of 93.8% of cases were reported to occur during co-sleeping/bed sharing with parents, and in 72.8% of the cases, the infants were covered with the same quilt as their parents. In our study, most inhalation suffocation deaths (88.3%) involved liquid food (such as breast milk and formula milk). A total of 80.5% of infant deaths were reported to occur after eating; in 28.2% of those cases, the infants were held upright and patted by their caregivers, and in 57.2% of them, were laid down to sleep immediately after eating.Conclusions: To reduce the occurrence of unintentional suffocation, local government should strengthen knowledge and awareness of unintentional suffocation prevention and safety among parents and caregivers, healthcare providers should educate parents and caregivers about safety issues of unintentional suffocation and relevant policies should be introduced to provide environments and activities that reduce the risk of suffocation such as promoting the Safe to Sleep Campaign. It is important to enhance the focus on infant unintentional suffocation as a health issue.
Needs, gaps and opportunities for standard and e-mental health care among at-risk populations in the Asia Pacific in the context of COVID-19: A rapid scoping review.
Authors: Murphy Jill K. , Khan Amna , Sun Qiumeng , Minas Harry , Hatcher Simon , Ng Chee H , Withers Mellissa , Greenshaw Andrew , Michalak Erin E , Chakraborty Promit Ananyo , Sandanasamy Karen Sharmini , Ibrahim Nurashikin , Ravindran Arun , Chen Jun , Nguyen Vu Cong , Lam Raymond W 
Published: April 2021

Abstract
Background: The COVID-19 pandemic is expected to have profound mental health impact, including in the Asia Pacific Economic Cooperation (APEC) region. Some populations might be at higher risk of experiencing negative mental health impacts and may encounter increased barriers to accessing mental health care. The pandemic and related restrictions have led to changes in care delivery, including a rapid shift to the use of e-mental health and digital technologies. It is therefore essential to consider needs and opportunities for equitable mental health care delivery to the most at-risk populations. This rapid scoping review: 1) identifies populations in the APEC region that are at higher risk of the negative mental health impacts of COVID-19, 2) identifies needs and gaps in access to standard and e-mental health care among these populations, and 3) explores the potential of e-mental health to address these needs.Methods: We conducted a rapid scoping review following the PRISMA Extension for Scoping Reviews (PRISMA-ScR). We searched Medline, Embase and PsychInfo databases and Google Scholar using a search strategy developed in consultation with a biomedical librarian. We included records related to mental health or psychosocial risk factors and COVID-19 among at-risk groups; that referred to one or more APEC member economies or had a global, thus generalizable, scope; English language papers, and papers with full text available.Results: A total of 132 records published between December 2019 and August 2020 were included in the final analysis. Several priority as-risk populations, risk factors, challenges and recommendations for standard and e-mental health care were identified. Results demonstrate that e-mental health care can be a viable option for care delivery but that specific accessibility and acceptability considerations must be considered. Options for in-person, hybrid or “low-tech” care must also remain available.Conclusions: The COVID-19 pandemic has highlighted the urgent need for equitable standard and e-mental health care. It has also highlighted the persistent social and structural inequities that contribute to poor mental health. The APEC region is vast and diverse; findings from the region can guide policy and practice in the delivery of equitable mental health care in the region and beyond.
A Transitional Fossil Mite (Astigmata: Levantoglyphidae fam. n.) from the Early Cretaceous Suggests Gradual Evolution of Phoresy-Related Metamorphosis
Authors: Klimov Pavel B. , Vorontsov Dmitry D. , Azar Dany , Sidorchuk Ekaterina A. , Braig Henk R. , Khaustov Alexander A. , Tolstikov Andrey V. 
Published: April 2021

Abstract
Metamorphosis is a key innovation allowing the same species to inhabit different environments and accomplish different functions, leading to evolutionary success in many animal groups. Astigmata is a megadiverse lineage of mites that expanded into a great number of habitats via associations with invertebrate and vertebrate hosts (human associates include stored food mites, house dust mites, and scabies). The evolutionary success of Astigmata is linked to phoresy-related metamorphosis, namely the origin of the heteromorphic deutonymph, which is highly specialized for phoresy (dispersal on hosts). However, the origin of this instar is enigmatic since it is morphologically divergent and no intermediate forms are known. Here we describe the heteromorphic deutonymph of Levantoglyphus sidorchukae n. gen. and sp. (Levantoglyphidae fam. n.) from early Cretaceous amber of Lebanon (129 Ma), which displays a transitional morphology ("missing link"). It is similar to extant phoretic deutonymphs in its modifications for phoresy but has the masticatory system and other parts of the gnathosoma well-developed. These aspects point to a gradual evolution of the astigmatid heteromorphic morphology and metamorphosis. The presence of well-developed presumably host-seeking sensory elements on the gnathosoma suggests that the deutonymph was not feeding either during phoretic or pre- or postphoretic periods.
A Comparison between W score and Ryan score in diagnosing of Laryngopharyngeal reflux disease
Authors: Wang Gang , Wang Lei , Sun Zhezhe , Guo Yuzhu , Liu Hongdan , Han Haolun , Zhong Changqing , Sui Xinke , Li Baowei , Zhou Ying , Qu Changmin , Li Lianyong , Sataloff Robert T. , Wu Jimin , Wang Qi , Fang Jugao , Lv Qiuping , Huang Yijiang , Wu Wei 
Published: April 2021

Abstract
ObjectiveTo assess the diagnostic value of W score which was supposed to identify laryngopharyngeal reflux disease (LPRD) patients from the normal population by Dx-pH monitoring, comparing with Ryan score.MethodsOne hundred and eight patients with suspected LPRD and complete follow-up results after more than 8 weeks of anti-reflux therapy were enrolled from the Department of Otolaryngology-Head and Neck Surgery, Gastroenterology and Respiratory Medicine of seven hospitals. Their Dx-pH monitoring data before treatment were reanalyzed to obtain the W score besides Ryan score and then the diagnostic sensitivity and specificity were compared according to the result of anti-reflux therapy.ResultsEighty-seven (80.6%) cases were anti-reflux therapy effective, and 21 patients (19.4%) were ineffective. Twenty-seven patients (25.0%) had a positive Ryan score. The W score was positive in 79 (73.1%) patients. There were 52 patients who had a negative Ryan score, but a positive W score. The diagnostic sensitivity, specificity, positive predictive value and negative predictive value of the Ryan score were 28.7%, 90.5%, 92.6% and 23.5%, respectively (kappa = 0.092, p = 0.068), whereas those of the W score for LPRD were 83.9%, 71.4%, 92.4% and 51.7%, respectively (kappa = 0.484, p < 0.001).ConclusionsW score is much more sensitive for the diagnosis of LPRD. Prospective studies with larger patient populations are necessary to validate and improve the new diagnostic criteria.Trial RegistrationChinese Clinical Trial Registry: ChiCTR1800014931.
Development and Validation of a Between Primary PLA2R-Negative Membranous Nephropathy and Minimal Change Disease Confirmed by Renal Biopsy
Authors: Wu Feng , Zhang Yiding , Cui Wen , Dong Yijun , Geng Yingyang , Liu Changhao , Li Zemeng , Xie Yandong , Cai Xiaojing , Shang Jin , Xiao Jing , Zhao Zhanzheng 
Published: April 2021

Abstract
Background: Membranous nephropathy (MN) and minimal change disease (MCD) are two common causes leading to nephrotic syndrome (NS). They have similar clinical feature but different treatment strategy and prognosis. M-type phospholipase A2 receptor (PLA2R) is considered as a specific marker of membranous nephropathy. However, its sensitivity is only about 70%. Therefore, there is a lack of effective and noninvasive tools to distinguish PLA2R-negative MN and MCD patients without renal biopsy. We aim to develop a discrimination model using noninvasive parameters for distinguishing the two diseases and support immediate treatment before result of renal biopsy.Methods: A total 949 patients who were pathologically diagnosed as idiopathic MN or primary MCD in the First Affiliated Hospital of Zhengzhou University from January 2017 to August 2019 were enrolled in this study, including 805 idiopathic MN (605 PLAR2-positive MN and 200 PLA2R-negative MN) and 144 primary MCD. Based on the basic information and laboratory examination of 200 PLA2R-negative MN and 144 MCD, we used a univariate and multivariate logistic regression to select the relevant variables and develop the discrimination model. ROC curve and calibration curve were used to evaluate the diagnostic ability and calibration ability of the model. The decision curve was used to calculate the net benefit. We also test the effectiveness of our model in all 949 patients.Results: A novel model that included age, albumin, urea, high density lipoprotein, urea and red blood cell count was established for PLA2R-negative MN and MCD. The discrimination model has excellent differential capability (an AUC of 0.889 in training group and an AUC of 0.920 in test group) and calibration capability. When testing in all 949 patients, our model also showed good discrimination ability for all idiopathic MN and MCD.Conclusion: We constructed a discrimination model with high diagnostic effectiveness for PLA2R-negative MN and MCD. The model could also be used for all idiopathic MN and MCD patients.