bluuebunny/crossref_metadata_2025_split huggingface dataset:
Yeast-expressed Recombinant SARS-CoV-2 Receptor Binding Domain, RBD203-N1 as a COVID-19 Protein Vaccine Candidate
Authors: Chen Wen-Hsiang , Pollet Jeroen , Strych Ulrich , Lee Jungsoon , Liu Zhuyun , Kundu Rakhi Tyagi , Versteeg Leroy , Villar Maria Jose , Adhikari Rakesh , Wei Junfei , Poveda Cristina , Keegan Brian , Bailey Aaron Oakley , Chen Yi-Lin , Gillespie Portia M. , Kimata Jason T. , Zhan Bin , Hotez Peter J. , Bottazzi Maria Elena 
Published: August 2021

ABSTRACTBackgroundSARS-CoV-2 protein subunit vaccines are being evaluated by multiple manufacturers to fill the need for low-cost, easy to scale, safe, and effective COVID-19 vaccines for global access. Vaccine candidates relying on the receptor-binding domain (RBD) of the SARS-CoV-2 spike protein have been the focus of our development program. In this paper, we report on the generation of the RBD203-N1 yeast expression construct, which produces a recombinant protein that when formulated with alum and the TLR-9 agonist, CpG1826 elicits a robust immune response and protection in mice.MethodThe RBD203-N1 antigen was expressed in the yeast Pichia pastoris X33. After fermentation at the 5 L scale, the protein was purified by hydrophobic interaction chromatography followed by anion exchange chromatography. The purified protein was characterized biophysically and biochemically, and after its formulation, the immunogenicity and efficacy were evaluated in mice.Results, Conclusions, and SignificanceThe RBD203-N1 production process yielded 492.9 ± 3.0 mg/L of protein in the fermentation supernatant. A two-step purification process produced a >96% pure protein with a recovery rate of 55 ± 3% (total yield of purified protein: 270.5 ± 13.2 mg/L fermentation supernatant). The protein was characterized as a homogeneous monomer with well-defined secondary structure, thermally stable, antigenic, and when adjuvanted on alum and CpG, it was immunogenic and induced robust levels of neutralizing antibodies against SARS-CoV-2 pseudovirus. These characteristics show that this vaccine candidate is well suited for technology transfer with feasibility of its transition into the clinic to evaluate its immunogenicity and safety in humans.
Sensory Modality and Spoken Language Shape Reading Network in Blind Readers of Braille
Authors: Tian Mengyu , Saccone Elizabeth J. , Kim Judy S. , Kanjlia Shipra , Bedny Marina 
Published: August 2021

AbstractThe neural basis of reading is highly consistent across many languages and scripts. Are there alternative neural routes to reading? How does the sensory modality of symbols (tactile vs. visual) influence their neural representations? We examined these questions by comparing reading of visual print (sighted group, n=19) and tactile Braille (congenitally blind group, n=19). Blind and sighted readers were presented with written (words, consonant strings, non-letter shapes) and spoken stimuli (words, backward speech) that varied in word-likeness. Consistent with prior work, the ventral occipitotemporal cortex (vOTC) was active during Braille and visual reading. A posterior/anterior vOTC word-form gradient was observed only in sighted readers with more anterior regions preferring larger orthographic units (words). No such gradient was observed in blind readers. Consistent with connectivity predictions, in blind compared to sighted readers, posterior parietal cortices were recruited to a greater degree and contained word-preferring patches. Lateralization of Braille in blind readers was predicted by laterality of spoken language and reading hand. The effect of spoken language increased along a cortical hierarchy, whereas effect of reading-hand waned. These results suggested that the neural basis of reading is influenced by symbol modality and spoken language and support connectivity-based views of cortical function.
Circulation of third-generation cephalosporin resistant Salmonella Typhi in Mumbai, India
Authors: Argimón Silvia , Nagaraj Geetha , Shamanna Varun , Sravani Darmavaram , Vasanth Ashwini Kodlipet , Prasanna Akshatha , Poojary Aruna , Bari Anurag Kumar , Underwood Anthony , Kekre Mihir , Baker Stephen , Aanensen David M. , Lingegowda Ravikumar Kadahalli 
Published: August 2021

AbstractWe report the persistent circulation of third-generation cephalosporin resistant Salmonella Typhi in Mumbai, linked to the acquisition and maintenance of a previously characterized IncX3 plasmid carrying the ESBL gene blaSHV-12 and the fluoroquinolone resistance gene qnrB7 in the genetic context of a triple mutant also associated with fluoroquinolone resistance.
Prochlorococcus exudate stimulates heterotrophic bacterial competition with rival phytoplankton for available nitrogen
Authors: Calfee Benjamin C. , Glasgo Liz D. , Zinser Erik R. 
Published: August 2021

AbstractThe marine cyanobacterium Prochlorococcus numerically dominates the phytoplankton community of the nutrient-limited open ocean, establishing itself as the most abundant photosynthetic organism on Earth. This ecological success has been attributed to lower cell quotas for limiting nutrients, superior resource acquisition, and other advantages associated with cell size reduction and genome streamlining. In this study we tested the prediction that Prochlorococcus outcompetes its rivals for scarce nutrients, and that this advantage leads to its numerical success in nutrient-limited waters. Strains of Prochlorococcus and its sister genus Synechococcus grew well in both mono- and co-culture when nutrients were replete. However, in nitrogen-limited medium Prochlorococcus outgrew Synechococcus, but only when heterotrophic bacteria were also present. In the nitrogen-limited medium, the heterotroph Alteromonas macleodii outcompeted Synechococcus for nitrogen, but only if stimulated by exudate released by Prochlorococcus, or if a proxy organic carbon source was provided. Analysis of a nitrate reductase mutant Alteromonas suggested that Alteromonas outcompetes Synechococcus for nitrate, during which co-cultured Prochlorococcus grows on ammonia or other available nitrogen species. We propose that Prochlorococcus can stimulate antagonism between heterotrophic bacteria and potential phytoplankton competitors through a metabolic cross-feeding interaction, and this stimulation could contribute to the numerical success of Prochlorococcus in the nutrient-limited regions of the ocean.Significance StatementIn nutrient-poor habitats, the competition for limited resources is thought to select for organisms with enhanced ability to scavenge nutrients and utilize them efficiently. Such adaptations characterize the cyanobacterium Prochlorococcus, the most abundant photosynthetic organism in the nutrient-limited open ocean. In this study the competitive superiority of Prochlorococcus over a rival cyanobacterium, Synechococcus, was captured in laboratory culture. Critically, this outcome was achieved only when key aspects of the open ocean were simulated: a limited supply of nitrogen, and the presence of heterotrophic bacteria. Results indicate that Prochlorococcus promotes its numerical dominance over Synechococcus by energizing the heterotroph’s ability to outcompete Synechococcus for available nitrogen. This study demonstrates how interactions between trophic groups can influence interactions within trophic groups.
A mechanistic switch from selective transporter to an ion channel impairs the filamentation signalling capability of ammonium transceptors in yeast
Authors: Williamson Gordon , Brito Ana Sofia , Bizior Adriana , Tamburrino Giulia , Mirandela Gaëtan Dias , Harris Thomas , Hoskisson Paul A. , Zachariae Ulrich , Marini Anna Maria , Boeckstaens Mélanie , Javelle Arnaud 
Published: August 2021

AbstractAmmonium translocation through biological membranes by the ubiquitous Amt-Mep-Rh family of transporters plays a key role in all domains of life. Two highly conserved histidine residues protrude into the lumen of these transporters, forming the family’s characteristic Twin-His motif. It has been hypothesized that the motif is essential to confer the selectivity of the transport mechanism. Here, using a combination of in vitro electrophysiology, in vivo yeast functional complementation and in silico molecular dynamics simulations, we demonstrate that variations in the Twin-His motif trigger a mechanistic switch between a specific transporter, depending on ammonium deprotonation, to an unspecific ion channel activity. We therefore propose that there is no selective filter that governs the specificity in Amt-Mep transporters but the inherent mechanism of translocation, dependent on the fragmentation of the substrate, ensures the high specificity of the translocation. We further show that both mechanisms coexist in fungal Mep2 Twin-His variants, disrupting the transceptor function and so inhibiting the filamentation process. These data strongly support a transport mechanism-mediated signalling process in the long-standing debate on the sensory function of Mep2-like transporters.
Cytogenetic evidence supports Avena insularis being closely related to hexaploid oats
Authors: Fominaya Araceli , Loarce Yolanda , González Juan M. , Ferrer Esther 
Published: August 2021

AbstractCytogenetic observations, phylogenetic studies and genome analysis using high-density genetic markers have suggested a tetraploid Avena species carrying the C and D genomes (formerly C and A) to be the donor of all hexaploid oats (AACCDD). However, controversy surrounds which of the three extant CCDD tetraploid species - A. insularis, A. maroccana and A. murphyi - is most closely related to hexaploid oats. The present work describes a comparative karyotype analysis of these three CCDD tetraploid species and two hexaploid species, A. sativa and A. byzantina. This involved the use of FISH with six simple sequence repeats (SSRs) with the motifs CT, AAC, AAG, ACG, ATC and ACT, two repeated ribosomal sequences, and C genome-specific repetitive DNA. The hybridization pattern of A. insularis with oligonucleotide (AC)10 was also determined and compared with those previously published for A. sativa and A. byzantina. Significant differences in the 5S sites and SSR hybridization patterns of A. murphyi compared to the other CCDD species rule out its being directly involved in the origin of the hexaploids. In contrast, the repetitive and SSR hybridization patterns shown by the D genome chromosomes, and by most of the C genome chromosomes of A. maroccana and A. insularis, can be equated with the corresponding chromosomes of the hexaploids. Several chromosome hybridization signals seen for A. insularis, but not for A. maroccana, were shared with the hexaploid oats species, especially with A. byzantina. These diagnostic signals add weight to the idea that the extant A. insularis, or a direct ancestor of it, is the most closely related progenitor of hexaploid oats. The similarity of the chromosome hybridization patterns of the hexaploids and CCDD tetraploids was taken as being indicative of homology. A common chromosome nomenclature for CCDD species based on that of the hexaploid species is proposed.
Improved protein docking by predicted interface residues
Authors: Pozzati Gabriele , Kundrotas Petras , Elofsson Arne 
Published: August 2021

ABSTRACTScoring docking solutions is a difficult task, and many methods have been developed for this purpose. In docking, only a handful of the hundreds of thousands of models generated by docking algorithms are acceptable, causing difficulties when developing scoring functions. Today’s best scoring functions can significantly increase the number of top-ranked models but still fails for most targets. Here, we examine the possibility of utilising predicted residues on a protein-protein interface to score docking models generated during the scan stage of a docking algorithm. Many methods have been developed to infer the portions of a protein surface that interact with another protein, but most have not been benchmarked using docking algorithms. Different interface prediction methods are systematically tested for scoring >300.000 low-resolution rigid-body template free docking decoys. Overall we find that BIPSPI is the best method to identify interface amino acids and score docking solutions. Further, using BIPSPI provides better docking results than state of the art scoring functions, with >12% of first ranked docking models being acceptable. Additional experiments indicated precision as a high-importance metric when estimating interface prediction quality, focusing on docking constraints production. We also discussed several limitations for the adoption of interface predictions as constraints in a docking protocol.
Cryo-EM Structures of CTP Synthase Filaments Reveal Mechanism of pH-Sensitive Assembly During Budding Yeast Starvation
Authors: Hansen Jesse M , Horowitz Avital , Lynch Eric M , Farrell Daniel P , Quispe Joel , DiMaio Frank , Kollman Justin M 
Published: August 2021

ABSTRACTMany metabolic enzymes self-assemble into micron-scale filaments to organize and regulate metabolism. The appearance of these assemblies often coincides with large metabolic changes as in development, cancer, and stress. Yeast undergo cytoplasmic acidification upon starvation, triggering the assembly of many metabolic enzymes into filaments. However, it is unclear how these filaments assemble at the molecular level and what their role is in the yeast starvation response. CTP Synthase (CTPS) assembles into metabolic filaments across many species. Here, we characterize in vitro polymerization and investigate in vivo consequences of CTPS assembly in yeast. Cryo-EM structures reveal a pH-sensitive assembly mechanism and highly ordered filament bundles that stabilize an inactive state of the enzyme, features unique to yeast CTPS. Disruption of filaments in cells with non-assembly or hyper-assembly mutations decreases growth rate, reflecting the importance of regulated CTPS filament assembly in homeotstasis.
Teaming up in entrepreneurship education: does the team formation mode matter?
Authors: Warhuus Jan P. , Günzel-Jensen Franziska , Robinson Sarah , Neergaard Helle 
Published: August 2021

PurposeThis paper investigates the importance of team formation in entrepreneurship education, and the authors ask: how do different team formation strategies influence teamwork in higher education experiential learning-based entrepreneurship courses?Design/methodology/approachEmploying a multiple case study design, the authors examine 38 student teams from three different entrepreneurship courses with different team formation paths to uncover potential links between team formation and learning outcomes.FindingsThe authors find that team formation mode matters. Randomly assigned teams, while diverse, struggle with handling uncertainty and feedback from potential stakeholders. In contrast, student self-selected teams are less diverse but more robust in handling this pressure. Results suggest that in randomly assigned teams, the entrepreneurial project becomes the team's sole reference point for well-being. Seeking to protect the project, the team's ability to deal with uncertainty and external feedback is limited, stifling development. In student self-select teams, team well-being becomes a discrete reference point. This enables these teams to respond effectively to external project feedback while nurturing team well-being independently.Originality/valueEducation theories' implications about the benefit of team diversity may not apply to experiential learning-based entrepreneurship education's typical level of ambiguity and uncertainty. Therefore, educators may have to reconsider the unique dynamics of team formation strategies to ensure strong teamwork and teamwork outcomes.
The impact of contingency fit on organisational performance: an empirical study
Authors: Amhalhal Abdallah , Anchor John , Tipi Nicoleta S. , Elgazzar Sara 
Published: August 2021

PurposeThe research investigates the effectiveness of the performance measurement alignment approach which claims that measurement diversity (multiple performance measures) should be aligned with organisational contingencies to enhance organisational performance.Design/methodology/approachThe theoretical framework is contingency theory. The study is an empirical investigation of the indirect relationship between three contextual factors (business strategy, information technology and organisation size) and organisational performance via multiple performance measures. The results are derived from cross-sectional questionnaire survey data from 132 Libyan companies (response rate of 61%). For data analysis, the research uses mediation regression analysis via Preacher and Hayes' (2004) macro.FindingsThere is a significant indirect effect of business strategy and information technology, but not organisation size, on organisational performance. The measurement diversity approach plays a core mediating role in the relationship between the contingencies and organisational performance.Practical implicationsThe study helps to provide a better understanding of the usefulness of the fit/match between contingencies and Multiple Performance Measures in improving organisational performance.Originality/valueThe empirical evidence supports the central proposition of contingency theory that there is no universally appropriate performance measurement system which applies equally to all organisations in all circumstances. It also provides evidence relating to non–manufacturing and an emerging market context. This research significantly extends the relevant literature by highlighting the relationship between information technology, multiple performance measures and organisational performance. This study is the first to use Preacher and Hayes' (2004) macro to analyse mediation design in the field of contingency-based performance measurement.
Job meaningfulness, employee engagement, supervisory support and job performance: a moderated-mediation analysis
Authors: Panda Abinash , Sinha Subhashis , Jain Nikunj Kumar 
Published: August 2021

PurposeGuided by social exchange, broaden and build and conservation of resources theoretical perspectives, this study explores the moderated mediating role of supervisory support (SS) on the relationship between job meaningfulness (JM) on job performance (JP) through employee engagement (EE).Design/methodology/approachField data were collected from two hundred and nineteen executives and their thirty-eight supervisors of a large paint manufacturing industry through a time-lagged research design and was analyzed with partial least squares based structural equation modeling.FindingsFindings of this study indicate that JM mediated by EE contributes to JP, which means if an employee finds one's job meaningful, she/he is likely to be more engaged emotionally, psychologically and cognitively to deliver better JP. SS is also found to be salient as it moderates both direct and indirect relationships between JM and JP through EE.Research limitations/implicationsGeneralizability of the findings of this study should be done with caution. Though the study has time-laggard data from two different sources but missing longitudinal data restricts causality of relationships/findings.Practical implicationsThese findings are relevant for organizations given that organizational leaders can create a context, by appropriate job design and engaging work context that motivates employees to perform better in their jobs. Insights of this study will be useful for organizations to curate meaningful jobs for their employees and also groom leaders with requisite skills and competencies to help subordinates perform up to their potential.Originality/valueThis study is an attempt toward a better understanding of the interplay of JM, work engagement and SS on JP in a manufacturing set-up in India, which has not been hitherto examined in Indian context.
Fretting fatigue behavior and failure characteristics of 35CrMoA steel under elliptical loading path
Authors: Huang Ziao , Liu Xiaoshan , He Guoqiu , Zhou Zhiqiang , Ge Bin , Le Peiwen , Pan Jiaqi , Xu Xiaojun 
Published: August 2021

Purpose
This study aims to understand the multiaxial fretting fatigue, wear and fracture characteristics of 35CrMoA steel under the elliptical loading path.

Design/methodology/approach
By keeping the contact pressure and torsional shear cyclic stress amplitude unchanged; the axial cyclic stress amplitude varied from 650 MPa to 850 MPa. The fretting fatigue test was carried out on MTS809 testing machine, and the axial cyclic strain response and fatigue life of the material were analyzed. The fretting zone and fracture surface morphology were observed by scanning electron microscope. The composition of wear debris was detected by energy dispersive X-ray spectrometer.

Findings
In this study, with the increase of axial stress amplitude, 35CrMoA steel will be continuously softened, and the cyclic softening degree increases. The fretting fatigue life decreases unevenly. The fretting scars in the stick region are elongated in the axial direction. The area of fracture crack propagation zone decreases. In addition, the results indicate that wear debris in the slip region is spherical and has higher oxygen content.

Originality/value
There were few literatures about the multiaxial fretting fatigue behavior of 35CrMoA steel, and most scholars focused on the contact pressure. This paper reveals the effect of axial cyclic stress on fretting fatigue and wear of 35CrMoA steel under the elliptical loading path.
Empowerment of rural young people in informal farm entrepreneurship: the role of corporate social responsibility in Nigeria’s oil producing communities
Authors: Uduji Joseph Ikechukwu , Okolo-Obasi Elda Nduka 
Published: August 2021

Purpose
The purpose of this paper is to critically examine the corporate social responsibility (CSR) initiatives of multinational oil companies in Nigeria. Its main focus is to investigate the impact of the global memorandum of understanding (GMoU) on equipping rural young people with essential farming skills and knowledge for the adoption and application of modern agricultural inputs in the Niger Delta region.

Design/methodology/approach
This paper adopts a survey research technique, aimed at gathering information from a representative sample of the population, as it is essentially cross-sectional, describing and interpreting the current situation. A total of 800 rural young people were sampled across the oil producing region.

Findings
The results from the use of combined propensity score matching and logit model indicate that the GMoU model has a significant impact on the development of informal farm entrepreneurship generally, but somewhat undermined rural young people in the targeted agricultural clusters.

Practical implications
This suggests that youth-specific CSR farm projects can be effective in providing young people with the extra push needed to tackle the knowledge gap and poor agronomic that erect the below-per yield and lack of competitiveness of small-holder farmers in the region.

Social implications
It implies that a coherent and integrated CSR response from the business would be necessary to unlock investment opportunities on young people in farms for agricultural competitiveness and food security in Africa.

Originality/value
This study adds to the literature on informal farm entrepreneurship and rural communities’ debate in sub-Saharan Africa. It concludes that business has obligation to help in solving problems of youth unemployment in developing countries.