bluuebunny/crossref_metadata_2025_split huggingface dataset:
Exogenous and endogenous triggers differentially stimulate Pigr expression and antibacterial secretory immunity in the murine respiratory tract
Authors: Pausder Alexander , Fricke Jennifer , Schughart Klaus , Schreiber Jens , Strowig Till , Bruder Dunja , Boehme Julia Désirée 
Published: August 2021

Abstract
PurposeTransport of secretory immunoglobulin A (sIgA) through the airway epithelial cell barrier into the mucosal lumen by the polymeric immunoglobulin receptor (pIgR) is an important mechanism of respiratory mucosal host defense. Identification of immunomodulating substances that regulate secretory immunity might have therapeutic implications with regard to an improved immune exclusion. Thus, we sought to analyze secretory immunity under homeostatic and immunomodulating conditions in different compartments of the murine upper and lower respiratory tract (URT&LRT). MethodsPigr gene expression in lung, trachea and nasal-associated lymphoid tissue (NALT) of germ-free mice, specific-pathogen-free mice, mice with an undefined microbiome as well as LPS- and IFN-γ-treated mice was determined by quantitative real-time RT-PCR. IgA levels in bronchoalveolar lavage (BAL), nasal lavage (NAL) and serum were determined by ELISA. LPS- and IFN-γ-treated mice were colonized with Streptococcus pneumoniae and bacterial CFUs were determined in URT and LRT. ResultsRespiratory Pigr expression and IgA levels were dependent on the degree of exposure to environmental microbial stimuli. While immunostimulation with LPS and IFN-γ differentially impact respiratory Pigr expression and sIgA in URT vs . LRT, only prophylactic IFN-γ treatment reduces nasal colonization with S. pneumoniae . ConclusionAirway-associated secretory immunity can be partly modulated by exposure to microbial ligands and proinflammatory stimuli. Prophylactic IFN-γ-treatment significantly improves antibacterial immunity in the URT.
Rescuing Epileptic and Behavioral Alterations in a Dravet Syndrome Mouse Model by Inhibiting Eukaryotic Elongation Factor 2 Kinase (eEF2K)
Authors: Beratta Stefania , Ponzoni Luisa , Gritti Laura , Scalmani Paolo , Mantegazza Massimo , Sala Mariaelvina , Verpelli Chiara , Sala Carlo 
Published: August 2021

Abstract
Background: Dravet Syndrome is a severe childhood pharmaco-resistant epileptic disorder mainly caused by mutations in the SCN1A gene, which encodes for the α1 subunit of the type I voltage-gated sodium channel (NaV1.1), that cause imbalance between excitation and inhibition in the brain. We recently found that eEF2K knock out mice displayed enhanced GABAergic transmission and tonic inhibition and were less susceptible to epileptic seizures. Thus, we investigated the effect of inhibition of eEF2K on the epileptic and behavioral phenotype of Scn1a+/- mice, a murine model of Dravet Syndrome.Methods: To elucidate the role of eEF2K pathway in the etiopathology of Dravet syndrome we generated a new mouse model deleting the eEF2K gene in Scn1a+/- mice. By crossing Scn1a+/- mice with eEF2K-/- mice we obtained the three main genotypes needed for our studies, Scn1a+/+eEF2K+/+ (WT mice), Scn1a+/-eEF2K+/+ mice (Scn1a+/- mice) and Scn1a+/-eEF2K-/- mice, that were fully characterized for EEG and behavioral phenotype. Furthermore, we tested the ability of a pharmacological inhibitor of eEF2K in rescuing EEG alterations of the Scn1a+/- mice.Results: We showed that the activity of eEF2K/eEF2 pathway was enhanced in Scn1a+/- mice. Then, we demonstrated that both genetic deletion and pharmacological inhibition of eEF2K were sufficient to ameliorate the epileptic phenotype of Scn1a+/- mice. Interestingly we also found that motor coordination defect, memory impairments, and stereotyped behavior of the Scn1a+/- mice were reverted by eEF2K deletion. The analysis of spontaneous inhibitory postsynaptic currents (sIPSCs) suggested that the rescue of the pathological phenotype was driven by the potentiation of GABAergic synapses. Limitations: Even if we found that eEF2K deletion was able to increase inhibitory synapses function, the molecular mechanism underlining the inhibition of eEF2K/eEF2 pathway in rescuing epileptic and behavioral alterations in the Scn1a+/- needs further investigations.Conclusions: Our data indicate that pharmacological inhibition of eEF2K could represent a novel therapeutic intervention for treating epilepsy and related comorbidities in the Dravet syndrome.
Bio-Tolerance Potential and Environmental Risks Assessment of Oreochromis Niloticus and Lpomoea Aquatica in Agodi Reservoir, Nigeria
Authors: Ogungbile Peter , O Ajibare A. , Ayeku Patrick , Akinola Joshua 
Published: August 2021

Abstract
The concentration of nine heavy metals in Oreochromis niloticus and Ipomoea aquatica inhabiting Agodi reservoir, Oyo State, Nigeria were investigated for twelve months. The concentrations of the metals were carried out using PG990 Atomic Absorption Spectrophotometer. The Ecological Risk Quotient (ERQ) was calculated using standard methods while the tolerability was determined with Box Plot analysis. The metal bioaccumulation in O. niloticus and I. aquatica followed the order Mn>Fe>Zn>Cu>Co>Pb>Cd>Cr>Ni and Fe>Zn>Mn>Cu>Pb>Cd>Co>Cr>Ni respectively. The results revealed O. niloticus to bioaccumulate the metals more than I. aquatica. Most of the examined metals were higher than the safety limit for the metals concentration in O. niloticus and I. aquatica. Also, I. aquatica had higher tolerability for heavy metals than O. niloticus. In O. niloticus, metal concentration, as well as ERQ, was higher in the wet season while no particular order was observed for I. aquatica. The ERQ result revealed that Cd, Fe, Mn, Zn, Cu and Pb exhibited a high level of ecological risk to both the aquatic flora and fauna as the ERQ values were above the risk limit of one (1). Thus, there is a significant environmental risk associated with heavy metals in the water body.
Formation Mechanism of "Encapsulated" Carbide Slag Composite Alkalinity Sustained-release Particles for the Source Control of Acid Mine Drainage
Authors: Bai Jichi , Zhang Haiqin , Xiao Liping 
Published: August 2021

Abstract
Acid mine drainage (AMD) has caused serious and long-lasting damage to the environment in many countries. The source control of AMD is considered to be the most direct and effective method of remediation. Carbide slag is an industrial waste which is considered as a potential treatment material for AMD treatment due to the strong alkalinity. However, the application of carbide slag in the source control of AMD encounters difficulties due to its fast alkalinity release rate. This is a first attempt to mix carbide slag with bentonite to prepare alkalinity sustained-release particles for the source control of AMD. Results showed that the gel phase(C-S-H) formed between the interface of carbide slag and bentonite encapsulated the surface of the Ca(OH)2 which grain decreases from 267nm to 211nm. The optimum preparation conditions are suggested as follows: a mass ratio of bentonite to carbide slag of 3:7, a Na2CO3 dose of 10 wt%, and a calcination temperature of 500°C for 1 h. The particles can remove 105 mg/g Cu2 + within 12 h, and the loss rate is only 7.8%. Compared with carbide slag, the alkalinity release time of the particles is prolonged by 4 times.
Circ_0018534 Promotes Non-Small Cell Lung Cancer Progression by Upregulating BTBD7 via Sponging miR-153-3p
Authors: Bai Haoran , Zhu Lihua , Li Hegen , Zhou Lei 
Published: August 2021

Abstract
Background: Non-small cell lung cancer (NSCLC) is the most common malignant tumor in lung. In this study, we aimed to explore the role and underlying mechanism of circular RNA 0018534 (circ_0018534) in NSCLC progression.Methods: Real-time quantitative PCR (RT-qPCR) was used to detect the expression of circ_0018534, microRNA-153-3p (miR-153-3p) and BTB/POZ domain-containing protein 7 (BTBD7) in NSCLC tissues and cells. Protein expression was measured by western blot analysis. The morphological features of obtained NSCLC patient tissues were observed by haematoxylin and eosin staining assay. 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT), 5-Ethynyl-29-deoxyuridine, flow cytometry analysis, and transwell migration and invasion assays were used to examine cell viability, proliferation, apoptosis, migration and invasion, respectively. Bioinformatics analysis and dual-luciferase reporter assay were carried out to determine the interaction among circ_0018534, miR-153-3p and BTBD7. Furthermore, lentivector for short hairpin RNA against circ_0018534 (sh-circ_0018534) was used to decrease circ_0018534 expression in an animal tumor model.Results: Circ_0018534 and BTBD7 expression were significantly increased, whereas miR-153-3p expression was decreased in NSCLC tissues and cells. Circ_0018534 downregulation markedly alleviated cell proliferation, migration, invasion, and elevated cell apoptosis in NSCLC cells in vitro. MiR-153-3p inhibitors partially reversed the effects of circ_0018534 knockdown on cell proliferation, migration, invasion and apoptosis in NSCLC. Moreover, miR-153-3p could directly target BTBD7, and circ_0018534 promoted BTBD7 expression by targeting miR-153-3p. Besides, BTBD7 overexpression attenuated miR-153-3p mimics-mediated effects on NSCLC cell progression. Furthermore, circ_0018534 deletion suppressed NSCLC tumor growth in vivo.Conclusion: Our results demonstrated that circ_0018534 might contribute to the progression of NSCLC by miR-153-3p/BTBD7 axis, providing a potential target for the treatment of NSCLC.
Efficacy Analysis of One-stage Posterior-only Surgical Treatment for Thoracic Spinal Tuberculosis in the T4-6 Segments with Minimum 5-year Follow-up
Authors: Zhan Yi , Kang Xin , Gao Wenjie , Zhang Xinliang , Kong Lingbo , Hao Dingjun , Wang Biao 
Published: August 2021

Abstract
In recent years, with the in-depth research on spinal tuberculosis, posterior surgery alone has been praised highly by more and more surgeons due to the better correction of kyphosis, better maintenance of spinal physiological curvature, smaller surgical trauma and fewer surgical complications. However, there is currently lack of relevant reports about the efficacy of posterior surgery alone in the treatment of tuberculosis in the T4-6 segments. This study aimed to evaluate the clinical study efficacy and feasibility of one-stage posterior-only surgical treatment for thoracic spinal tuberculosis in the T4-6 segments. 67 patients with tuberculosis in T4-6 segments who underwent one-stage posterior-only surgery were included in this study. The clinical efficacy was evaluated using statistical analysis based on the data about erythrocyte sedimentation rate (ESR), C-reactive protein (CRP), Oswestry Dability Index (ODI) score, Visual Analogue Scale (VAS) score and Cobb angle before surgery, after surgery and at the last follow-up. All patients completed fusion during the follow-up period of 6–9 months. ESR and CRP were returned to normal for all patients at 6 months follow-up. In the meanwhile, among the 27 patients combined with neurological impairment, neurological functions of 22 cases (81.48%) recovered completely at the last follow-up (P < 0.05). Cobb angle of the kyphosis was improved from preoperative 34.8 ± 10.9° to postoperative 9.6 ± 2.8°, maintaining at 11.3 ± 3.2° at the last follow-up, The ODI and VAS scores were improved by 77.10% and 81.70%, respectively. This 5-year follow-up study shows that better clinical efficacy can be achieved for tuberculosis in T4-6 segments using one-stage posterior-only approach by costotransverse debridement in combination with bone graft and internal fixation. The posterior surgical method cannot only effectively accomplish debridement, obtain satisfactory clinical results, but also well correct kyphotic deformity and maintain it.
Inhibition of Proliferation in Ovarian Cancer Cell Line (PA-1) By The Action of Green Compound ‘Betanin’
Authors: R Rakshanaa , Shafreen Mohiraa , KUMAR NITIN 
Published: August 2021

Abstract
Ovarian carcinoma has a cure rate of 30% which makes it deadlier than any other disease. There are a number of genetic and epigenetic changes that lead to ovarian carcinoma cell transformation. Chemoprevention of cancer through application of natural compounds is the need of present generation as other methods are rigorous and have many side effects. Betanin, a compound from Beta vulgaris extract is used in present study to check its potential for inhibition of (PA-1) cancer cell proliferation. Determination of IC50 values through MTT assay was carried out, in addition measurement of Mitochondrial Membrane Potential (MMP), effect of Reactive Oxygen Species (ROS) generation and induction of Apoptosis in ovarian cancer cells through betanin was also observed. Results have shown betanin as a potential candidate for inhibition of ovarian cancer cell proliferation and it can be taken up as a serious compound for further studies for its application in cancer cure.
Intermolecular Interactions Between Serine and C60, C59Si, and C59Ge: A DFT Study
Authors: Mohammadi Mohsen Doust , Abdullah Hewa Y. 
Published: August 2021

Abstract
The study of intermolecular interactions is of great importance. This study attempted to quantitatively examine the interactions between Serine (C3H7NO3) and fullerene nanocages, C60, in vacuum. As the frequent introduction of elements as impurities into the structure of nanomaterials can increase the intensity of intermolecular interactions, nanocages doped with silicon and germanium have also been studied as adsorbents, C59Si and C59Ge. Quantum mechanical studies of such systems are possible in the density functional theory (DFT) framework. For this purpose, various functionals, such as B3LYP-D3, ωB97XD, and M062X, have been used. One of the most suitable basis functionals for the systems studied in this research is 6-311G (d), which has been used in both optimization calculations and calculations related to wave function analyses. The main part of this work is the study of various analyses that reveal the nature of the intermolecular interactions between the two components introduced above. The results of conceptual DFT, natural bond orbital, non-covalent interactions, and quantum theory of atoms in molecules were consistent and in favor of physical adsorption in all systems. Germanium had more adsorption energy than other dopants. The HOMO–LUMO energy gaps were as follows: C60: 5.996, C59Si: 5.309, and C59Ge: 5.188 eV at B3LYP-D3/6-311G (d) model chemistry. The sensitivity of the adsorption increased when an amino acid molecule interacted with doped C60, and this capability could be used to design nanocarrier to detect Serine amino acid.
Design and Analysis of Posterior Semicircular Canal BPPV Diagnostic Test Based on Physical Simulation
Authors: Yang Xiaokai , Yang Qiancheng , Liu Zhaobang 
Published: August 2021

Abstract
To discusses and analyzes how to realize the design of posterior semicircular canal BPPV diagnostic maneuver. First, measure the spatial attitude of the human semicircular canal, establish a BPPV virtual simulation platform, then analyze the key positions of the maneuver, and finally design a new diagnostic maneuver according to the demand, and perform physical simulation verification. The average value of the unit normal vector of the right posterior semicircular plane is [ 0.660, 0.702, 0.266], after rotate -46.8 ° around Z axis and 15.4 ° around Y axis, it parallel to the X axis. After that, when the tilt back angle reaches 70 °, the free otoconia in the left utricle will fall into the common crus; when bend forward 53.3°, the unit normal vector of the crista ampullaris plane of the posterior semicircular canal to the XY plane; when bend forward angle reaches 30°, the otoconia slides to the opening of the ampulla; when bend forward angle reaches 70°, the otoconia slides to the bottom of the crista ampullaris. The shallow pitching Yang maneuver is designed as turn head 45° to the one side, bend forward 45°, tilt back 90°, and bend forward 90°. The deep pitching Yang maneuver is designed as bend forward 90°, turn head 45° to one side, tilt back 135°, and bend forward 90°. A new posterior semicircular BPPV diagnostic test is designed to make the induced nystagmus have the characteristics of long latency, reversal, and repeatability, will not cause the inhibitory stimulation of the contralateral superior semicircular canal, and has good operation fault tolerance, which is of great value for clinical and scientific research.
Cell Viability And Electrical Response of Breast Cancer Cell Treated In Aquoes Graphene Oxide Solution Deposition On Interdigitated Electrode
Authors: Ramli Muhammad M. , Rosman Anis , Mazlan Nur , Ahmad Mohd , Halin Dewi Che , Mohamed Rafeezul , Osman Nurul , Reshak Ali 
Published: August 2021

Abstract
Breast cancer is one of the most reported cancers that can lead to death. Despite the advances in diagnosis and treatment procedures, the possibility of cancer recurrences is still high in many cases. With that in consideration, researchers from all over the world are showing interest in the unique features of Graphene oxide (GO), such as its excellent and versatile physicochemical properties, to explore further its potential and benefits towards breast cancer cell treatment. In this study, the cell viability and electrical response of GO, in terms of resistivity and impedance towards the breast cancer cells (MCF7) and normal breast cells (MCF10a), were investigated by varying the pH and concentration of GO. Firstly, the numbers of MCF7 and MCF10a were measured after being treated with GO for 24 and 48 hours. Next, the electrical responses of these cells were evaluated by using interdigitated electrodes (IDEs) gold electrodes that are connected to an E4980 20 Hz – 2 MHz Precision LCR meter. Based on the results obtained, as the pH of GO increased from pH 5 to pH 7, the number of viable MCF7 cells decreased while the number of viable MCF10a slightly increased after the incubation period of 48 hours. Similarly, the MCF7 also experienced higher cytotoxicity effects when treated with a GO concentration of more than 25 μg/mL. The findings from the electrical characterization of the cells observed that the number of viable cells has corresponded to the impedance of the cells. The electrical impedance of MCF7 decreased as the number of highly insulating viable cells membrane decreased, but in contrast, the electrical impedance of MCF10a increased as the number of highly insulating viable cells membrane increased. Hence, it can be deduced that the GO with higher pH and concentration impacts the MCF7 cancer cell line and MCF10a normal breast cell.
Questioning Abenomics and a Keynesian Approach to Japan’s Deflation Problem via Input-Output Analysis
Authors: BIYIK ONUR 
Published: August 2021

Abstract
Japan’s economy has had chronic deflation and high public debt problems so that it has been trying to handle its problems based on either the demand-side or the supply-side for more than two decades. This paper suggests that a perspective of Keynesian-inspired Japanese economic policy may positively impact a specific part of the economy such as demand-side solutions, as mentioned in Keynes’ theory. The Japanese economy is examined by applying the Input-Output (I/O) method in which the aggregated eleven-sector is arranged by using statistical data from the Ministry of Economy, Trade, and Industry (METI). Firstly, our analysis focuses mainly on the updated 2012 domestic I/O table in order to understand the Japanese economic structure by examining final demand elements and eleven sectors, associated with the Leontief method. Secondly, this paper then investigated comparing with Abenomics representing by a mix of the new Keynesian and neoliberal policies in order to comparatively analyzed between the current economic structure and the economic policy and lastly provided some discussions to figure out in perspective of how to solve some economic issues. Therefore, not only did this paper delineate three scenarios/keys through dominant final demand which account for the public consumption expenditures (PUCE) and the public gross fixed capital formation (PUGFC); the target of the growth rate -the automobile sector through PUGFC-, the employment rate -the commerce sector through PUCE-, and the import rate -the commerce sector through PUCE- based on the results of the data analyzed, but also comparatively examined Abenomics in order to provide some possible policy paths which highlight the demand-side remedies for the economic issues; structural reform in the fiscal sustainability, mainly wage increase (especially low and middle incomes), working practice (bringing back the mutual work environment), and fair tax system.
Winner and Loser in Terms of the FTAs and the Trade War: Case Study of the Japanese Market
Authors: BIYIK ONUR 
Published: August 2021

Abstract
The US raised trade war issues under protecting national security against China in July 2018. Likewise, the trade war has spread out across other regions such as India, the EU, Canada, Mexico, Russia, and Turkey through an additional tariff on products such as steel and aluminum. Clearly, the uncertainty has shown an increase since this friction created a pessimistic environment for the future world economy and did hurt economic development. Therefore, it has had negative effects for welfare -especially those (low-income consumers) who prefer to buy cheap imported goods. Contrary to protectionism, Japan has signed new FTAs with the EU and the US. In that context, this paper quantitatively examines the Japanese new FTAs under the trade war. It employs the general equilibrium approaches to not only investigate the economic structure of each country trade flow but also address the FTAs and the impacts of the welfare and sectoral value chains of the trade war. Essentially, the paper scenarios depend on the official list of the FTAs and the trade war-related goods. As a result of the FTAs under the trade war, the new Japanese trade agreements have provided some opportunities for its market as well as targeted countries. For instance, the Japanese benefit from the EU-Japan FTA would be $4.11 billion U.S.D. and the EU would gain $768 million U.S.D. within the 15-year. Moreover, the US not only would get a huge advance but also could get back its export market share from Pacific island nations in Japan when Japan would eliminate the tariff on concerned sectors for the US goods. For example, the US and Japan would improve their welfare by $4.09 billion U.S.D. and $398 million U.S.D., respectively through the limited USA-Japan FTA. That is, the US market would comparatively earn much more than Japan. Lastly, those who participate in the FTAs would boost their GDP, welfare, and value-added (productivity). For example, not only would Japan provide some opportunities for its market and then enhance its welfare and GDP, but also the EU and the US would boost their macro variables. However, from the perspective of the other regions/countries, those regions/countries which are not in the trade deal could lose their export market share in Japan, the US, and the UE and would, therefore, have a negative impact on their GDP and welfare.
Feature Selection and Scaling for Random Forest Powered Malware Detection System
Authors: Tripathi Ashutosh , Bhoj Naman , Khari Mayank , Pandey Bishwajeet 
Published: August 2021

Abstract
With the rise of internet usage malwares pose a great threat to user security and privacy. Therefore, to mitigate the problem it is essential to develop an efficient malware detection framework. In our research we experimented with various machine learning and feature scaling algorithms. Chi-Square was used as the feature selection technique which selected a set of 48 features from a feature space of 128 features. The empirical results provide us with conclusive evidence that Random Forest is the best algorithm for detection of malware achieving an accuracy of 91.300% on our dataset followed by Gradient Boosting and Support Vector Machines.