bluuebunny/crossref_metadata_2025_split huggingface dataset:
16S rDNA Sequencing analysis in identification of some multidrug resistant (MDR) bacterial isolates from clinical specimens
Authors: Iliyasu M.Y. , Bamanga R.A. , Umar A.F. , Agbo E.B. , Uba A. 
Published: May 2021

Accurate identification of bacterial pathogens from clinical specimens and Multidrug Resistant (MDR) characterization is a key to empirical therapy. Twelve (12) bacteria isolates from blood, urine and faecal samples were selected based on the ability to grow on Luria Bertani (LB) agar medium containing 100μg/ml ampicillin, identified by 16S rDNA PCR and sequencing. Identified isolates tagged; U01, U02, U03, U04, S08, U10 and U11 were from urine specimens, S05, S06, S07 and S12 from stool, while B09 was from blood. The isolates were screened for MDR pattern according to Kirby-Bauer disc diffusion method. Conventional biochemical tests revealed that all the isolates are Escherichia coli. The 16S gene sequencing results confirmed that, ten (10) isolates had high similar sequence alignment with identified E. coli strains, while two are Enterobacter cloacae and P. aeruginosa. The antimicrobial susceptibility pattern shows that, most of the isolates (83.3%) were MDR. All the 12 isolates (100%) are resistant to Ampicillin, Cephalothin, Erythromycin, Fusidic acid, Novobiocin and Oxacillin, but sensitive to Colistin sulphate and Imipenem. Eleven isolates (91.7%) are resistant to Chloramphenicol, Cotrimoxazole, Streptomycin, Sulphatriad and Tetracycline. Eight of the 12 isolates (66.7%) are resistant to Ciprofloxacin and Ceftriaxone. Seven (58.3%) are resistant to Cefotaxime, Cefuroxime and Gentamycin. Nine (75%) are sensitive and three isolates (25%) are resistant to Augmentin. The high resistance to these antibacterial agents in this study was due to the indiscriminate use of first-line common antibiotics like ampicillin in the study area, which is now substituted with Augmentin. Routine biochemical identification tests should always be confirmed with genotypic methods such as 16S gene sequencing, to avoid misdiagnosis, as variations do exist among some bacterial strains.
Keywords: Multidrug resistance, sequencing, 16S rDNA, E. coli, Enterobacter cloacae, P. aeruginosa.
“ANALISIS PELAYANAN FUNGSI PENDAMPINGAN PASTORAL DI GKPPD LIANG JERING RESORT ACEH TENGGARA TAHUN 2019”
Authors: Sinamo Arjun 
Published: May 2021

Abstrak - Penelitian ini bertujuan untuk mendeskripsikan pelaksanaan pendampingan pastoral dan hambatan serta menganalisis 5 (lima) fungsi pendampingan pastoral di jemaat GKPPD Liang Jering. Penelitian ini menggunakan jenis penelitian kualitatif. Proses pengumpulan data dilakukan melalui wawancara atau inteview. Adapun wawancara yang dilakukan, bertujuan untuk mengetahui hal-hal sebagai berikut:Mendeskripsikan proses bagaimana teknik-teknik pelayanan pastoral berjalan di jemaat GKPPD Liang JeringMengidentifikasi penyebab kurangnya pelaksanaan penerapan fungsi pendampingan pastoral dan menganalisis efektivitas pelayanan pastoral di GKPPD Liang JeringSumber data yang diperoleh dalam penelitian ini adalah Pendeta, Guru Kuria, Sekretaris Kuria, dan Bendahara Kuria. Hasil analisis menunjukkan bahwa fungsi pendampingan pastoral masih sangat kurang dilakukan oleh pelayan di jemaat GKPPD Liang Jering. Kurangnya pelaksanaan pendampingan di jemaat GKPPD Liang Jering ini karena pendeta yang berstatus PNS dan tidak tinggal di Liang Jering dan kurangnya pengetahuan tentang pastoral terhadap pengendeng kuria. Hal ini dilihat dari hasil wawancara kepada pendeta dan pengendeng kuria, serta observasi di gereja GKPPD Liang Jering Resort Aceh Tenggara. Dari hasil penelitian dapat disimpulkan bahwa pendampingan pastoral adalah hal yang sangat dibutuhkan oleh jemaat GKPPD Liang Jering, tetapi karena adanya hambatan dan keterbatasan waktu sehingga pelaksanaan pendampingan pastoral tidak terlaksana dengan baik. Menurut penulis dari 5 (lima) fungsi pendampingan pastoral, ada hanya ada satu fungsi yang dilaksanakan di jemaat GKPPD Liang Jering Resort Aceh Tenggara yaitu fungsi mendukung.Kata Kunci : Pendampingan Pastoral, Fungsi Pendampingan Pastoral
Pengembangan Naskah Drama Materi Menjadi Berkat Bagi Sesama Dalam Menumbuhkan Sikap Berbagi
Authors: Tamba Sartika S 
Published: May 2021

The purpose of this study was to develop drama scripts in Menjadi Berkat Bagi Sesama in developing attitudes in fostering sharing in grade 5 students through Christian religion education. Sample is 61 teachers PAK IN Kec. Tarutung and Kec. Sipoholon, where 31 teachers as research and 30 teachers for instrument test. This research using development research with procedure model ADDIE which was developed Dick Carry, namely Analysis, Design, Development, Implementation, and Evaluation. Based on the research it was concluded that the drama script is very necessary as a learning medium by PAK teachers when teaching material Menjadi Berkat Bagi Sesama in fostering shared attitudes for students.
PENGARUH VARIASI PENGAJARAN PAK TERHADAP HASIL BELAJAR SISWA KELAS VIII SMP NEGERI 2 BALIGE TAHUN PEMBELAJARAN 2019/2020
Authors: Ritonga Sari Arta 
Published: May 2021

This study aims to determine the effect of Christian Religion Education teaching variations on students’ learning outcomes of Class VIII of SMP Negeri 2 Balige Academic Year 2019/2020, with the hypothesis that there is a positive and significant influence on Christian Religion Education teaching variations on students’ learning outcomes of class VIII of SMP Negeri 2 Balige Academic Year 2019/2020. This study uses inferential quantitative descriptive approach, with a population of all Protestant Christian students in class VIII of SMP Negeri 2 Balige, totaling 221 people with a sample of 60 people. Data were collected with a closed questionnaire of 20 items and a test of 20 items compiled by the author based on variable indicators according to expert theory. The research instrument trials were conducted on 30 students who were not research respondents, and its validity and reliability were tested. The results of the data analysis showed that there was a positive and significant influence on Christian Religion Education teaching variations on students’ learning outcomes of class VIII of SMP Negeri 2 Balige Academic Year 2019/2020 with a coefficient of determination (r²) = 25.90% and a significance test of influence obtained tcoun ttable = 6.097 2,000 , meaning that H0 is rejected and Ha is accepted. Keywords: Christian Religion Education teaching variations, Learning Outcomes.
Pengaruh Lingkungan Keluarga Kristen Terhadap Perkembangan Moral Anak
Authors: Hutajulu Anastesya Anggelika 
Published: May 2021

AbstrakPenelitian ini bertujuan mengetahui pengaruh lingkungan keluarga Kristen terhadap perkembangan moral anak usia 10-12 tahun di HKBP Partalitoruan Resort Hutabarat tahun 2020. Penelitian ini regresi dengan teknik Statistik Deskriptif. Populasi seluruh anak usia 10-12 tahun di HKBP Partalitoruan Resort Hutabarat tahun 2020 yaitu 40 orang. Sampel penelitian adalah seluruh populasi. Instrumen penelitian angket tertutup. Nilai rhitung=0,708 rtabel=0,312 dan thitung=6,180 ttabel=2,021 menunjukkan adanya hubungan lingkungan keluarga Kristen dengan perkembangan moral anak dan persamaan regresi . Diperoleh Fhitung=38,038Ftabel=1,51 maka H0 ditolak dan Ha diterima yaitu terdapat pengaruh yang positif dan signifikan antara lingkungan keluarga Kristen terhadap perkembangan moral anak usia 10-12 tahun di HKBP Partalitoruan Resort Hutabarat tahun 2020 sebesar 50,12%.Kata Kunci: lingkungan keluarga kristen, perkembangan moral anak. AbstractThe aim of study was to determine the effect of the Christian family environment on children moral development of aged 10-12 years at HKBP Partalitoruan Resort Hutabarat in 2020. This study used regression with descriptive statistical techniques. The population were 40 participants, all children who aged 10-12 years at HKBP Partalitoruan Resort Hutabarat in 2020. The research sample was the entire population. The study used closed questionnaire as research instrument. The statistic calculation showed that value of rcount was greater than ttable (0.708 0.312) and tcount was higher than ttable (6.180 2.021) indicated that there was a relationship between the Christian family environment and children moral development. The regression equation was obtained that value of Fcount was greater than Ftable (38.038 1.51) then H0 was rejected and Ha was accepted. It means there was a positive and significant effect between the Christian family environment on children moral development of aged 10-12 years at HKBP Partalitoruan Resort Hutabarat in 2020. The effect was to 50.12%.Keywords: Christian Family Environment, Children Moral Development.
Kajian Syair dan Bentuk Struktur Lagu Kekuatan Serta Penghiburan Pada Prodi PMG IAKN Tarutung
Authors: Situmeang Mona Wenni 
Published: May 2021

AbstrakKajian syair dan bentuk struktur lagu No. 332 Kekuatan Serta Penghiburan pada prodi PMG IAKN tarutung. Tujuan dari penelitian ini adalah untuk mengetahui lebih banyak mengenai pengembangan kajian yang terdapat dalam lagu kekuatan serta penghiburan dan mengetahui bentu-bentuk dan struktur lagu yang di teliti. Penelitian ini menggunakan metode kajian pustaka dengan pendekatan kualitatif. Adapun proses kerja yang dilakukan yaitu pengamatan, wawancara, dokumentasi, studi pustaka (online) dan analisis. Penelitian dilakukan dengan mengumpulkan informasi melalui penelitian di lapangan untuk memproleh hasil yang diolah menjadi data pasti. Dalam hal ini untuk pengembangan dalam bernyanyi dan memainkan organ. Dalam aspek musikologi, penulis mengambil salah satu lagu yang di nyanyikan oleh jemaat dalam ibadah yaitu lagu kekuatan serta penghiburan dengan nada dasar es=do, birama 4/4 tempo andante, (Mm 69) dan perubahan moderato (Mm80).lagu tersebut ditulis lagi dengan not balok kemudian di analisis dengan melihat aspe-aspek musik yaitu : melodi, tangga nada, ambitus, frase, motif, tempo, dan gaya vocal.Kata kunci : Kerajaan Allah, Kebenaran dan Pemeliharaan. Abstract The purpose of this study is to find out more about the development of studies that are in the song of strength and consolation and to know the forms and structures of the songs under study. This research uses literature review method with a qualitative approach. The work process carried out is observation, interview, documentation, literature study (online) and analysis. The Research Was Conducted By Gathering Information Through Field Research To Get Results That Are Processed Into Certain Data. In This Case For Development In Singing And Playing The Organ. In the aspect of musicology, the author of one of the songs sung by the congregation in worship is the song of strength and consolation with the basic tone of Es = Do, Birama 4/4 Tempo Andante, (Mm 69) and Moderato Changes (Mm80). The notes are then analyzed by looking at musical aspects, namely: melody, scale, ambitus, phrases, motifs, tempo, and vocal style.Keywords: Poetry study, form, song structure
Sikap Waspada Terhadap Ketamakan Akan Kekayaan (Lukas 12:13-21)
Authors: Imeldawati Tiur , Tarigan Iwan Setiawan , Sihombing Warseto Freddy 
Published: May 2021

AbstrakKetamakan akan kekayaandapat dimiliki oleh siapa saja, termasuk oleh orang percaya. Ketamakan dapat mengalihkan fokus orang percaya kepada hal-hal yang bersifat duniawi seperti mencari banyak harta dan menghalalkan segala cara untuk menjadi kaya. Dalam Injil Lukas, Yesus dengan jelas memberikan peringatan akan bahaya ketamakan yang dapat menjerumuskan orang ke dalam kebinasaan. Alkitab tidak melarang orang percaya menjadi kaya—kaya di hadapan Allah, itulah yang dikehendaki-Nya. Kekayaan adalah titipan yang diberikan Tuhan kepada umat-Nya. Perumpamaan yang Yesus mengajarkan bagaimana mengelola kekayaan untuk kemuliaan Allah,yang jauh lebih berkenan dari pada sekedar mengumpulkan kekayaan dan menikmatinya sendirian.Kata kunci : Ketamakan, mengumpulkan harta, kaya di hadapan Allah Abstract Greed for wealth can be owned by anyone, including Christians. But, greed can shift Christians’ focus to worldly things, like finding a lot of wealth and justifying any means to be rich. In Luke's Gospel, Jesus asserts against the dangers of greed can lead people to God’s destruction. The Bible does not forbid believers to be rich — rich before God, what He wills. Wealth is a gift God has given to His people. Jesus’ parable taught how to manage wealth for glory of God, is more pleasurable than accumulating wealth and enjoying it alone.Keywords: greedy, collect treasure, rich before God
To Disclose or Not to Disclose? Reasons for HIV Serostatus Disclosure and Non-disclosure by People Living with HIV/AIDS in Eastern India
Authors: Kansal Sangeeta , Sarkar Madhutandra , Kumar Alok , Chakravarty Jaya , Kumar Rakesh 
Published: May 2021

Background: Understanding the reasons for HIV serostatus disclosure and nondisclosure and how these reasons differ by certain characteristics of the people living with HIV/AIDS (PLWHA) is important for effective HIV prevention intervention strategies. Objectives: This study was undertaken to investigate/identify the reasons for disclosure and nondisclosure of HIV serostatus by PLWHA, and to find out any association between the reasons for disclosure or nondisclosure and certain demographic and clinical characteristics, i.e. age, gender, WHO clinical stage and CD4 count of the study population. Methods: A cross-sectional study was conducted among all patients of 18-49 years with confirmed HIV infection registered at the antiretroviral therapy (ART) center of a tertiary care hospital in eastern Uttar Pradesh, India for one year, from July 2017 to June 2018. Results: The most common reason for disclosure of HIV serostatus was the presence of any family member at the time of collection of HIV test report (68.5%), and the most common reason for not disclosing the serostatus was stigmatization (68%). The reasons for disclosure was found to be associated with the WHO clinical stage of the respondents (p <0.05). Conclusions: The findings of this study highlights the need for tailoring intervention strategies for improving disclosure decision making according to the specific needs of PLWHA. There is also a need to address the concerns of those who are reluctant to disclose. More emphasis should be given on creating awareness about HIV stigma, on the importance of serostatus disclosure and secondary HIV prevention in the community.
EVIDÊNCIAS CIENTÍFICAS ACERCA DA INDUÇÃO DE GENOTOXICIDADE PROVOCADA PELA HIDROXIURÉIA EM PACIENTES COM ANEMIA FALCIFORME
Authors: Lima Azenate de Sousa , Lustosa Jeiciane Rodrigues , Batista Nelson Jorge Carvalho 
Published: May 2021

A anemia falciforme (AF) é uma patologia crônica que possui fatores hereditários, descrita como autossômica recessiva, no qual as hemácias adquirem a forma de foice, uma condição mutagênica da proteína hemoglobina (Hb) encontrada na hemácia circulante no sangue e responsável pelo transporte de O2 e CO2. Analisar as evidências científicas acerca da indução de genotoxicidade provocada pela Hidroxiuréia (HU) em pacientes com doença falciforme. Trata-se de uma pesquisa de revisão integrativa, dos artigos disponíveis na íntegra, nos últimos 10 anos, dos idiomas português e inglês, com a estratégia de busca PICO. Os resultados obtidos, em populações com faixa etárias distintas, descrevem sendo possível uma instabilidade genômica que pode vir a possibilitar a mutação de células que posteriormente são denominadas carcinogênicas. O uso da HU, pode está causando alterações a nível hematológico e nas células de defesa, apresentando mielotoxicidade, induzindo a um processo de leucopenia e plaquetopenia de acordo com o tempo do uso do fármaco. De acordo com a análise comparativa dos estudos encontrados, pacientes com AF que fazem uso prolongado da HU, apresentam evidências de danos ao DNA.

Palavras-chave: Anemia falciforme. Hidroxiuréia. Teste de micronúcleo.
An Introduction to Fashion Retailing
Authors: Koumbis Dimitri 
Published: May 2021

If you’re taking your first steps into the fast-paced world of retail, then merchandiser, store designer, retailer and educator Dimitri Koumbis is the ideal guide. In An Introduction to Fashion Retailing, he’ll walk you through everything from the history of retail design, to the intricacies of consumer behaviour, fast fashion and corporate social responsibility. You’ll also learn professional techniques through detailed case studies of international retailers, including LVMH, Estée Lauder and ASOS.
This revised edition includes expanded coverage of omnichannel retail approaches and retail KPIs as well as an outline of future retail trends in brick and mortar, e-commerce and technology. There's also a whole new chapter introducing visual merchandising, expanding on the importance of the store's overall design and visual representation of products.
Atari Design
Authors: Guins Raiford 
Published: May 2021

Drawing from deep archival research and extensive interviews, Atari Design is a rich, historical study of how Atari’s industrial and graphic designers contributed to the development of the coin-op cabinet.
Innovative game design played a key role in the growth of Atari – from Pong to Asteroids and beyond – but fun, challenging, and exciting game play was not unique to the famous Silicon Valley company. What set it apart from its competitors was innovation in cabinet design. Atari did not just make games, it designed products for environments.
With “tasteful packaging”, Atari exceeded traditional locations like bars, amusement parks, and arcades, developing the look and feel of their game cabinets for new locations such as fast food restaurants, department stores, country clubs, university unions, and airports, making game-play a ubiquitous social and cultural experience. By actively shaping the interaction between user and machine, overcoming styling limitations and generating a distinct corporate identity, Atari designed products that impacted the everyday visual and material culture of the late 20th century.
Design was never an afterthought at Atari.
ВОКАЛЬНО ЕСТРАДНЕ МИСТЕЦТВО В ДИСКУРСІ ФОРМУВАННЯ МУЗИЧНОГО ЕТОСУ КУЛЬТУРИ
Authors: KАBLOVА Tetіаnа 
Published: May 2021

Метою даної роботи є розгляд концепції музичного етосу та обґрунтування ролі естрадного та вокального виконавства як явища для формування культурних цінностей найвищого рівня існування суспільства. Було проаналізовано значну кількість джерел, що дозволило визначити основні проблеми, що потребують вивчення, а саме вивчення естрадного мистецтва з позицій смислового навантаження щодо формування культурних цінностей. Методологія дослідження полягає у застосуванні порівняльного, історичного та логічного методів. Цей методологічний підхід дозволяє розкрити та проаналізувати певні погляди на українську популярну музику та її значення як культурний код швидкоплинних змін часу та значення цього виду мистецтва на шляху до позиції культурної цінності етосу. Встановлено, що культурний вектор вокальної та естрадної діяльності поширюється на антропологічний напрямок у контексті історичного та культурного сенсу існування суспільства, яке, в свою чергу, є носієм як колективне несвідоме в процесі сприйняття дійсності. Це дозволяє вивчати естрадно-вокальне мистецтво як втілення цінностей короткого проміжку часу і як тенденцію до визначення вокально-естрадного мистецтва як соціокультурного явища в історичному континуумі, що впливає на формування загальнолюдського музичного етосу. Близькість до соціальних подій людського життя, а також відповідність інтерактивному існуванню міжкультурної комунікації дозволяє нам стверджувати естрадно-вокальне мистецтво як спосіб виокремлення складової музичного етосу епохи. Визначено взаємозв'язок між соціальними та історичними факторами, які суттєво впливають на формування естрадно-вокального мистецтва, що дозволяє виокремити основні віяння та характерні погляди суспільства в короткий проміжок часу. Зазначено, що саме завдяки естрадно-вокальному мистецтву структурується певне підґрунтя для формування музичного етосу культури.
СЦЕНІЧНИЙ МЕТОД ВСЕВОЛОДА МЕЙЄРХОЛЬДА ТА ЙОГО ЗАСТОСУВАННЯ У ЦИРКОВОМУ МИСТЕЦТВІ
Authors: TYSHCHUK Veronica 
Published: May 2021

Доля, творчість, художня спадщина Всеволода Мейєрхольда володіють нескінченно привабливою силою. Невтомний винахідник, майстер режисури, який відкрив нові театральні горизонти, він пройшов нелегкий шлях, прокладаючи свою стежину на ниві театрального мистецтва, постійно відчуваючи невдоволеність, потяг до нових, більш дієвих засобів у мистецтві. Створюючи свій театр, свого актора, свою методологію, він заплатив життям за право театру бути театром. Творчість Всеволода Мейєрхольда – це виснажлива боротьба за право вийти на новий етап власної свободи. Театр, лідером якого раніше був драматург і актор, знайшов нового лідера – режисера. Автором театру став режисер.
Всеволод Мейєрхольд з невтомною енергією в організованому ним театральному просторі почав створювати повний, вичерпний запас засобів побудови художньої форми вистави. Він знайшов ці засоби у тілі артиста та мізансценах, будував композиції своїх вистав за законами музики. Руйнуючи естетику натуралістичного психологічного театру, Всеволод Мейєрхольд ввів біомеханіку як відчуження від психологічної правди на сцені і як форму тієї технології, яка вивільнила актора для іншого непсихологічного існування. З ім'ям Мейєрхольда пов’язане будівництво Нового Театру, нової, гнучкої і багатозмістовної театральної системи, яка прийшла на зміну старій, ренесансовій системі.
Побудова Нового театру неможлива без ідеї Нової системи гри у ньому. А для Нової системи гри обов’язково потрібен Новий актор, який у ній буде почувати себе, як риба у воді. А такого Нового актора треба виховати, розробивши Нову акторську систему виховання. Ці речі взаємопов’язані і одна без одної існувати не можуть. Тому Всеволод Мейєрхольд і розробив авангардну акторську техніку – знамениту біомеханіку, що вчить артиста організовувати та контролювати свій психофізичний апарат у просторі та часі сценічної дії