bluuebunny/crossref_metadata_2025_split huggingface dataset:
Optimization of the Extraction Process and Application of Bacterial Extracts in the Control of Brown Spot and Leaf Blast in Rice Culture
Authors: Arriel-Elias Marina Teixeira , Arriel Gabriel Carlos Teixeira Freire , Bezerra Gustavo Andrade , Santos Pedro Henrique Dias dos , Severino Vanessa Gisele Pasqualotto , Filippi Marta Cristina 
Published: December 2021

Abstract
The objective of this work was to optimize the extraction process and application of bacterial extracts of Bacillus sp. and Serratia sp. in leaf blast control (Magnapothe oryzae) and brown spot (Bipolaris oryzae) in rice culture. The work was divided into three stages: 1) Bacterial obtaining extracts through liquid-liquid extraction 2) Antagonistic capacity of bacterial extracts to M. oryaze and B. oryae 3) Suppression of brown spot (A1) and leaf blast (A2) in greenhouse. The bacterial isolates in present study were identified as Bacillus sp. (BRM32110) and Serratia marcescens (BRM32113). The crude extract of both isolates at different extraction times 6, 16, 24, 48 and 72 hours reduced the growth of colonies of M. oryzae and B. oryzae by up to 92% and 28%, respectively. The extracts that showed highest inhibition of colony growth were those obtained after 6 and 16h of incubation and were selected for subsequent assays. These, for both isolates were able to reduce conidia germination by up to 91% and apressorium formation of M. oryzae by up to 93%. In green house, A1 the treatment that stood out was the extract of Bacillus sp. (16h) with 6.7% of leaf area affected and in A2 the treatment S. marcescens extract (16h) stood out with only 7.6% of leaf area affected with brusone when compared to control. The use of extracts of Bacillus sp. and Serratia marcescens was efficient in reducing the severity of brown spot and leaf blast in rice crop.
Water deficit stress tolerance assessment in barley cultivars using drought tolerance indices
Authors: Habib Md , Mannan Md , Karim Md , Miah Md , Singh Hari 
Published: December 2021

Abstract
Crop productivity is greatly affected by drought stress. Understanding the drought tolerance capability of the crop varieties available in a country is the foremost consideration for drought adaptation. The objective of this research work was to examine the drought tolerance potentiality of 5 cultivated barley varieties (BARI Barley5, BARI Barley6, BARI Barley7, BARI Barley8 and BARI Barley9) through calculating drought tolerance indices. A completely randomized design (CRD) with three replications was followed in the experiment, where crops were grown under control (80% of FC) and water deficit environment (50% of FC). Stress Tolerance (TOL), Mean Productivity (MP), Geometric Mean Productivity (GMP), Stress Susceptibility Index (SSI), Stress Tolerance Index (STI), Harmonic Mean (HAM), Yield Index (YI) and Yield Stability Index (YSI) were calculated based on grain yield under control and drought conditions. BARI Barley7 and BARI Barley8 were the most tolerant variety and BARI Barley9 considered as susceptible based on TOL and SSI. Drought tolerance indices like MP, HAM, GMP, TOL as well as STI were showed a high correlation with grain yield under both conditions and were recognized as appropriate indices to identify varieties with high grain yield and low sensitivity to drought stress.
Recurrent Painful Ophthalmoplegic Neuropathy: Diagnostic Controversies. Single Case Report with a Systematic Review
Authors: Falsaperla Raffaele , Presti Santiago , Bianco Manuela Lo , Catanzaro Stefano , Marino Silvia , Ruggieri Martino 
Published: December 2021

Abstract
Background: Ophthalmoplegic migraine, renamed “Recurrent Painful Ophthalmoplegic Neuropathy (RPON) in 2013 by the International Headache Society is a rare neurologic disorder characterized by recurrent attacks of ophthalmoplegia associated to ipsilateral headache. The etiology is still unknown. Typical magnetic resonance imaging findings show a focal nerve thickening and contrast enhancement. In the majority of cases, there is a full recovery within days or weeks. There is no evidence supporting a specific treatment. The review defines the characteristics of the recurrent painful ophthalmoplegic neuropathy in patients within 2 years of age underlying the importance of the role of magnetic resonance imaging even in presence of the first attack. Thus, an emblematic case report is presented.Case presentation: The authors present a case of third cranial nerve paresis in a 17-month-old male child, presenting a neuroradiological pattern highly suggestive of schwannoma, aneurism or recurrent painful ophthalmoplegic neuropathy. Thus, a review of the literature with the pediatric casuistry of recurrent painful ophthalmoplegic neuropathy occurred within 2 years of age focusing on diagnostic considerations is presented. The authors highlight the importance to consider recurrent painful ophthalmoplegic neuropathy in presence of magnetic resonance imaging findings and clinical symptoms referable to aneurysm or schwannoma. Thus, the review defines the characteristics and the neuroradiological findings at the first RPON attack occurred under 2 years of age.Conclusion: Although two attacks are necessary, the review strongly suggests to consider recurrent painful ophthalmoplegic neuropathy even at the first attack, in presence of described characteristics and the aforementioned magnetic resonance imaging findings.
Preparation and Characterization of Novel Polymer-Based Heterogeneous MPg-C3N4 Catalyst and Its Application in Multi-Component Reactions
Authors: Ghafuri Hossein , Zare-Bakheir Ensiye , Hanifehnejad Peyman , Talebi Maryam , Ghanbari Nastaran 
Published: December 2021

Abstract
Magnetic polyvinyl alcohol-based g-C3N4 composite (MPg-C3N4) was synthesized as a heterogeneous catalyst. It was characterized by various analyses such as FT-IR, XRD, FE-SEM, TGA, EDX, and VSM. For showing the catalytic merits of MPg-C3N4, it was used in the synthesis of acridinedione and 4H-chromene derivatives in a highly efficient yield. Various aldehyde derivatives were used to show the merits of the MPg-C3N4. High yield, short reaction time, easy separation, mild condition reaction, simple purification, non-toxic solvents, and reusability are the advantages of using polymer-based heterogeneous MPg-C3N4 catalyst to synthesize acridinedione and 4H-chromene derivatives. Also, the intended products were purified by simple crystallization.
Development and Validation of Nomogram to Predict Very Early Recurrence of Combined Hepatocellular-Cholangiocarcinoma After Hepatic Resection: A Multi-Institutional Study
Authors: Wu Yijun , Liu Hongzhi , Zeng Jianxing , Chen Yifan , Fang Guoxu , Zhang Jingyu , Zhou Weiping , Zeng Yongyi , Liu Jingfeng 
Published: December 2021

Abstract
Background and Objectives
Combined hepatocellular cholangiocarcinoma (cHCC) has a high incidence of early recurrence. The objective of this study is to construct a model predicting very early recurrence (VER)(ie, recurrence within 6 months after surgery) of cHCC.
Methods
131 consecutive patients from Eastern Hepatobiliary Surgery Hospital served as a development cohort to construct a nomogram predicting VER by using multivariable logistic regression analysis. The model was internally and externally validated in an validation cohort of 90 patients from Mengchao Hepatobiliary Hospital using the C concordance statistic, calibration analysis and decision curve analysis (DCA).
Results
The VER nomogram contains microvascular invasion(MiVI), macrovascular invasion(MaVI) and CA19-9>25mAU/mL. The model shows good discrimination with C-indexes of 0.77 (95%CI: 0.69 - 0.85 ) and 0.76 (95%CI:0.66 - 0.86) in the development cohort and validation cohort respectively. Decision curve analysis demonstrated that the model are clinically useful and the calibration of our model was favorable. Our model stratified patients into two different risk groups, which exhibited significantly different VER.
Conclusions
Our model demonstrated favorable performance in predicting VER in cHCC patients.
Thermodynamic Surprises of Cu(II)-Amylin Complexes in Membrane Mimicking Solutions
Authors: Dzień Emilia , Dudek Dorota , Witkowska Danuta , Rowińska-Żyrek Magdalena 
Published: December 2021

Abstract
Membrane environment often has an important effect on the structure, and therefore also on the coordination mode of biologically relevant metal ions.This is also true in the case of Cu(II) coordination to amylin analogues – rat amylin, amylin1-19, pramlintide and Ac-pramlintide, which offer N-terminal amine groups and/or histidine imidazoles as copper(II) anchoring sites. Complex stabilities are comparable, with the exception of the very stable Cu(II)-amylin1-19, which proves that the presence of the amylin C-terminus lowers its affinity for copper(II); although not directly involved, its appropriate arrangement sterically prevents early metal binding.Most interestingly, in membrane-mimicking solution, the Cu(II) affinities of amylin analogues are lower than the ones in water, probably due to the crowding effect of the membrane solution and the fact that amide coordination occurs at higher pH, which happens most likely because the α-helical structure, imposed by the membrane-mimicking solvent, prevents the amides from binding at lower pH, requiring a local unwinding of the α-helix.
Has Green Finance Facilitated China's Low-Carbon Economic Transition?
Authors: Wenqi Li , Fan Jingjing , Zhao Jiawei 
Published: December 2021

Abstract
The development of green finance helps to promote the transition to a low-carbon economy. Using data from 30 provinces in China from 2001-2019, we empirically examine the impact of green finance on the transition to a low-carbon economy and further explore the mediating role of low-carbon technological innovation in this facilitation process. The study finds that (1) green finance can significantly contribute to the transformation of the low-carbon economy from China as a whole. However, when China is divided into four regions: east, central, west and northeast, the contribution of green finance to the low-carbon economic transition in the west is not significant. (2) After adding low-carbon technologies to the model, green finance still has a significant contribution to the low-carbon economic transition, but this contribution decreases with the intervention of low-carbon technologies. (3) There is a strong spatial dependence between green finance development and low-carbon economic transformation in 30 Chinese provinces. However, the dependence among provinces tends to weaken after 2011. And the overall green financial development in China shows a positive spillover effect on the low-carbon economic transition. Based on the analysis results, several countermeasures are proposed to promote the further development of China's low-carbon economy.
Phenotypic plasticity in adults of Anticarsia gemmatalis exposed to sub-doses of Bt-based bioinsecticide
Authors: Fernandes Fabricio Oliveira , de Souza Tamires Doroteo , Sanches Ariadne Costas , Carvalho Ivan Ricardo , Dias Naymã Pinto , Desiderio Janete Apparecida , Polanczyk Ricardo Antonio 
Published: December 2021

Abstract
Anticarsia gemmatalis Hünber, 1818 is one of the main defoliating species in the soybean crop. Bacillus thuringiensis Berliner, 1915, is a bacterium used in the biological control of this pest species. Resistant populations and their sublethal effects caused by the use of the bacteria have already been reported; however, there are no studies on phenotypic plasticity in adulthood exposed to Bt-based bioinsecticide sub-doses. This study aimed to evaluate the morphometry of A. gemmatalis adults under laboratory conditions submitted to the Bt-based bioinsecticide Dipel SC over the three generations. The body segments mensuread were width, length, and area of the anterior and posterior wings, the weight of the integument, chest, abdomen, wings, and the whole adult of males and females. Among the treatments, LC5 in the first generation and CL10 in the second generation were those with lower thresholds in relation to the weight of the chest and abdomen, considering the proportions of the body smaller than the females. The female’s weight adulthood was reduced by 10% about males, and, only in the first generation. Males have larger body size and more pronounced phenotypic plasticity than females. Here, we demonstrate the first study assessing the phenotypic plasticity of A. gemmatalis adults.
Radiological study of the nasal septal body
Authors: aydın canset , önay övsen , gözükara melih gaffar , Ulubay Hakan 
Published: December 2021

Abstract
Objectives

The nasal septal body (NSB) is the thickened area of the septum located superior to the inferior turbinates and anterior to the middle turbinates. NSB contributes to nasal breathing via functionally and anatomically. The aim of the present study was to analyze NSB size and its association with such variables as age, septal deviation, and nasal turbinate size
Material and Methods

This retrospective study included 381 randomly selected patients that underwent paranasal sinus CT between 2014 and 2019. NSB size, septal deviation angle, and middle and inferior turbinate size were analyzed.
Results

NSB, inferior turbinate, middle turbinate, and inferior turbinate-P were significantly smaller on the deviated side. NSB, inferior turbinate, middle turbinate, and inferior turbinate-P size according to nasal septal angle was also significantly smaller on the deviated side. NSB and inferior turbinate size was positively correlated.
Conclusion

The NSB is an important structure for the regulation of air flow in the nose and should be evaluated in routine examination of the nose especially before the nasal surgery.
Post-Diagnostic Beta Blocker Use and Breast Cancer-Specific Mortality: A Population-Based Cohort Study
Authors: Scott Oliver William , TinTin Sandar , Elwood J Mark , Cavadino Alana , Habel Laurel A , Kuper Marion , Campbell Ian , Lawrenson Ross 
Published: December 2021

Abstract
Purpose Beta blockers (BB) have been associated with improved, worsened, or unchanged breast cancer outcomes in previous studies. This study examines the association between the post-diagnostic use of BBs and death from breast cancer in a large, representative sample of New Zealand (NZ) women with breast cancer. Methods Women diagnosed with a first primary breast cancer between 2007 and 2016 were identified from four population-based regional NZ breast cancer registries and linked to national pharmaceutical data, hospital discharges, and death records. The median follow up time was 4.51 years. Cox proportional hazard models were used to estimate the hazard of breast cancer-specific death (BCD) associated with post-diagnostic BB use. Results Of the 14,976 women included in analyses, 21% used a BB after diagnosis. BB use (vs non-use) was associated with a small and non-statistically significant increased risk of BCD ( adjusted hazard ratio: 1.11; 95% CI: 0.95-1.29). A statistically significant increased risk confined to short-term use (0-3 months) was seen (HR=1.40; 1.14-1.73), and this risk steadily decreased with increasing duration of use and became a statistically significant protective effect at 3+ years of use (HR=0.54; 0.34-0.87). Conclusion Our findings suggest that any increased risk associated with BB use may be driven by risk in the initial few months of use. Long-term BB use may be associated with a reduction in BCD.
A Telemonitoring Programme in Patients with Heart Failure in France: A Cost-Utility Analysis
Authors: Caillon Mégane , Sabatier Rémi , Legallois Damien , Courouve Laurène , Donio Valérie , Boudevin Florence , de Chalus Thibault , Hauchard Karine , Belin Annette , Milliez Paul 
Published: December 2021

Abstract
Background

Certain telemedicine programmes for heart failure (HF) have been shown to reduce all-cause mortality and heart failure-related hospitalisations, but their cost-effectiveness remains controversial. The SCAD programme is a home-based interactive telemonitoring service for HF, which is one of the longest-running and largest telemonitoring programmes for HF in France. The objective of this cost-utility analysis was to evaluate the cost-effectiveness of the SCAD programme with respect to standard hospital-based care in patients with HF.
Methods

A Markov model simulating hospitalisations and mortality in patients with HF was constructed to estimate outcomes and costs. The model included six distinct health states (three ‘not hospitalised’ states, two ‘hospitalisation for heart failure’ states, both depending on the number of previous hospitalisations, and one death state. The model lifetime in the base case was ten years. Model inputs were based on published literature. Outputs (costs and QALYs) were compared between SCAD participants and standard care. Deterministic and probabilistic sensitivity analyses were performed to assess uncertainty in the input parameters of the model.
Results

The number of quality-adjusted life years (QALYs) was 3.75 in the standard care setting and 4.41 in the SCAD setting. This corresponds to a gain in QALYs provided by the SCAD programme of 0.65 over the ten-year lifetime of the model. The estimated total cost was €30,932 in the standard care setting and €35,177 in the SCAD setting, with an incremental cost of €4,245. The incremental cost-effectiveness ratio for the SCAD programme over standard care was estimated at €4,579/QALY. In the deterministic sensitivity analysis, the variables that had the most impact on the ICER were HF management costs. The likelihood of the SCAD programme being considered cost-effective was 90% at a willingness-to-pay threshold of €11,800.
Conclusions

Enrolment of patients into the SCAD programme is highly cost-effective. Extension of the programme to other hospitals and more patients would have a limited budget impact but provide important clinical benefits. This finding should also be taken into account in new public health policies aimed at encouraging a shift from inpatient to ambulatory care.
Spatio-temporally Varying Manning Roughness in Rivers and Streams: A calibration approach using in-situ water level and UAS altimetry
Authors: Liu Jun , Jiang Liguang , Bandini Filippo , Kittel Cécile Marie Margaretha , Balbarini Nicola , Hansted Nisha Gill , Grosen Henrik , Bauer-Gottwein Peter 
Published: December 2021

Abstract
Hydraulic roughness (expressed in terms of e.g. Manning's roughness coefficient) is an important input to hydraulic and hydrodynamic simulation models. One way to estimate roughness parameters is by hydraulic inversion, using observed water surface elevation (WSE) collected from gauging stations, satellite platforms or UAS (Unmanned Aerial System) −based altimeters. Specifically, UAS altimetry provides close to instantaneous observations of longitudinal profiles and seasonal variations of WSE for various river types, which are useful for calibrating roughness parameters. However, it is computationally expensive to run high−resolution hydrodynamic models for long simulation periods (e.g. multiple years), and thus global optimization of spatially and temporally distributed parameter sets for such models, e.g., spatio−temporally varying river roughness, is still challenging.This study presented an efficient calibration approach for hydraulic models, using a simplified steady-state hydraulic solver, UAS altimetry datasets, and in-situ observations. The calibration approach minimized the weighted sum of a misfit term, spatial smoothness penalty, and a sinusoidal a priori temporal variation constraint. The approach was first demonstrated for several synthetic calibration experiments and the results indicated that the global search algorithm accurately recovered the Manning–Strickler coefficients M for short river reaches in different seasons, and M varied significantly in time (due to the seasonal growth cycle of the aquatic vegetation) and space (due to, e.g. spatially variable vegetation density). Subsequently, the calibration approach was demonstrated for a real WSE dataset collected at a Danish test site, i.e., Vejle Å. Results indicated that spatio-temporal variation in M was required to accurately fit in-situ and UAS altimetry WSE observations. This study illustrated how UAS altimetry and hydraulic modeling can be combined to achieve improved understanding and better parameterization of small and medium-sized rivers, where conveyance is controlled by vegetation growth and other spatio-temporally variable factors.
Reporter System Controlled by the Involucrin Promoter as a Tool to Follow the Epidermal Differentiation
Authors: Fernandes Myrian Thiago Pruschinski , Santos Jeniffer Farias dos , Freitas Bruna Letícia , Reigado Gustavo Roncoli , Antunes Fernanda , Tessarollo Nayara , Chambergo Felipe Santiago , Strauss Bryan Eric , Nunes Viviane 
Published: December 2021

Abstract
Different approaches have been explored to study skin biology, including the use of stem cells. Mesenchymal stem cells (MSC) from umbilical cord can be safely and easily obtained, however a simple strategy to monitor their differentiation is essential. Involucrin is a marker of keratinocyte terminal differentiation, and its promoter (pINV) directs stratum-specific expression of this protein. We designed a reporter system containing EGFP under control of pINV to assess MSC differentiation into keratinocytes. The functional sequence of pINV was inserted into a lentiviral vector, originating LeGO-GpINV. MSC were transduced with the LeGO-GpINV and induced to differentiate into keratinocytes upon cultivation with Keratinocyte Serum Free Medium supplemented. MSC differentiation was confirmed by morphological changes and by the expression of epidermal markers, by flow cytometry, quantitative PCR and western blot. The activity of kallikreins 5, 6 and 7 was detected using fluorogenic substrates. After 14 days of differentiation, MSC transduced with LeGO-GpINV showed an increase in EGFP fluorescence and expressed CK10, CK14, involucrin and filaggrin. There was also an increase in the kallikrein activity. This reporter system allowed to temporally assess the epidermal differentiation, simultaneously with involucrin expression, opening perspectives for the in vivo study of skin biology and in regenerative medicine.