bluuebunny/crossref_metadata_2025_split huggingface dataset:
Timing and duration of light exposure during conidia development determine tolerance to ultraviolet radiation
Authors: Brancini Guilherme T P , Bachmann Luciano , Braga Gilberto Ú L 
Published: October 2021

ABSTRACTMetarhizium is an important genus of soil-inhabiting fungi that are used for the biological control of insects. The efficiency of biocontrol is dependent on the maintenance of inoculum viability under adverse field conditions such as solar ultraviolet (UV) radiation. Therefore, increasing the tolerance of Metarhizium to UV radiation is necessary. It was previously established that, in mycelium, exposure to visible light increases tolerance to UV radiation. Similarly, growth under visible light for 14 days induces the production of tolerant conidia. However, a study evaluating if and how visible light affects conidia and their relationship with UV radiation was never performed. Here, we report that a relatively short and timed exposure to light around the time of conidiation is sufficient to induce the production of conidia with increased photoreactivating capacity and UV tolerance in Metarhizium acridum. Conidia produced by this method retain their characteristic higher tolerance even after many days of being transferred to the dark. Furthermore, we show that mature conidia of M. acridum and Metarhizium brunneum can still answer to light and regulate UV tolerance, suggesting that gene expression is possible even in dormant spores. Being able to respond to light in the dormant stages of development is certainly an advantage conferring improved environmental persistence to Metarhizium.
Extracellular Domains of Transmembrane Proteins Defy the Expression Level–Evolutionary Rate Anticorrelation
Authors: Sarkar Chandra , Alvarez-Ponce David 
Published: October 2021

Abstract
Highly expressed proteins tend to evolve slowly, a trend known as the expression level–rate of evolution (E–R) anticorrelation. Whereas the reasons for this anticorrelation remain unclear, the most influential hypotheses attribute it to highly expressed proteins being subjected to strong selective pressures to avoid misfolding and/or misinteraction. In accordance with these hypotheses, work in our laboratory has recently shown that extracellular (secreted) proteins lack an E–R anticorrelation (or exhibit a weaker than usual E–R anticorrelation). Extracellular proteins are folded inside the endoplasmic reticulum, where enhanced quality control of folding mechanisms exist, and function in the extracellular space, where misinteraction is unlikely to occur or to produce deleterious effects. Transmembrane proteins contain both intracellular domains (which are folded and function in the cytosol) and extracellular domains (which complete their folding in the endoplasmic reticulum and function in the extracellular space). We thus hypothesized that the extracellular domains of transmembrane proteins should exhibit a weaker E–R anticorrelation than their intracellular domains. Our analyses of human, Saccharomyces and Arabidopsis transmembrane proteins allowed us to confirm our hypothesis. Our results are in agreement with models attributing the E–R anticorrelation to the deleterious effects of misfolding and/or misinteraction.
The Framing Effect of Intergenerational Comparison of Technologies on Technophobia Among Older Adults
Authors: Xi Wanyu , Zhang Xin , Ayalon Liat 
Published: October 2021

Abstract

Objectives
Sharing similar negative age stereotypes (e.g., outdated, unfashionable), older adults and older technologies are stereotypically associated with each other. This also was found to be internalized by older adults. Recent research has suggested that internalized negative age stereotypes may be one of the reasons for technophobia among older adults. Therefore, considering the pervasiveness of intergenerational comparison of technologies (e.g., computer vs. tablet) in which older-generation technologies are negatively portrayed, we aim to investigate whether a mere intergenerational comparison of technologies would affect technophobia via negative self-stereotypes activation among older adults. Specifically, 2 commonly seen framings of intergenerational comparison of technologies are examined: contrast framing, which describes opposing intergenerational relationship of technologies; and connect framing, which describes the continuous intergenerational relationship of technologies.

Methods
We designed 3 advertisements for a made-up new technological product using contrast framing, connect framing, and neutral framing (where intergenerational comparison was absent). A sample of 284 participants (aged 27–83 years) was gathered online and randomly assigned into the 3 experimental conditions. Self-perception of aging (SPA), technophobia, and potential covariates were measured.

Results
The results showed a significant framing × age × gender effect on psychosocial loss dimension of SPA and technophobia. Men were significantly affected by the framing effect as they age, but women were not affected. Contrast framing (vs. connect vs. neutral framing) led to significantly higher technophobia via the psychosocial loss dimension of SPA among older men aged 49 and older.

Discussion
The findings have important implications for how to better convey persuasive information to promote new technology adoption among older adults.
Sibling Deaths, Racial/Ethnic Disadvantage, and Dementia in Later Life
Authors: Cha Hyungmin , Thomas Patricia A , Umberson Debra 
Published: October 2021

Abstract

Objectives
Sibling loss is understudied in the bereavement and health literature. The present study considers whether experiencing the death of siblings in mid-to-late life is associated with subsequent dementia risk and how differential exposure to sibling losses by race/ethnicity may contribute to racial/ethnic disparities in dementia risk.

Methods
We use discrete-time hazard regression models, a formal mediation test, and a counterfactual simulation to reveal how sibling loss in mid-to-late life affects dementia incidence and whether unequal exposures by race/ethnicity mediate the racial/ethnic disparities in dementia. We analyze data from the Health and Retirement Study (2000–2016). The sample includes 13,589 respondents (10,670 non-Hispanic White, 1,761 non-Hispanic Black, and 1,158 Hispanic adults) aged 65 years and older in 2000 who show no evidence of dementia at baseline.

Results
Discrete-time hazard regression results show that sibling loss in mid-to-late life is associated with up to 54% higher risk for dementia. Sibling loss contributes to Black–White disparities in dementia risk. In addition, a simulation analysis shows that dementia rates would be 14% lower for Black adults if they experienced the lower rates of sibling loss experienced by White adults. This pattern was not observed among Hispanic adults.

Discussion
The death of a sibling in mid-to-late life is a stressor that is associated with increased dementia risk. Black adults are disadvantaged in that they are more likely than Whites to experience the death of siblings, and such losses contribute to the already substantial racial/ethnic disadvantage in dementia.
The microtubule-associated protein Tau suppresses the axonal distribution of PDF neuropeptide and mitochondria in circadian clock neurons
Authors: Zhang Melanie Y , Lear Bridget C , Allada Ravi 
Published: October 2021

Abstract
Disrupted circadian rhythms are a prominent feature of multiple neurodegenerative diseases. Yet mechanisms linking Tau to rhythmic behavior remain unclear. Here, we find that expression of a phosphomimetic human Tau mutant (TauE14) in Drosophila circadian pacemaker neurons disrupts free-running rhythmicity. While cell number and oscillations of the core clock protein PERIOD are unaffected in the small LNv (sLNv) neurons important for free running rhythms, we observe a near complete loss of the major LNv neuropeptide pigment dispersing factor (PDF) in the dorsal axonal projections of the sLNvs. This was accompanied by a ~50% reduction in the area of the dorsal terminals and a modest decrease in cell body PDF levels. Expression of wild-type Tau also reduced axonal PDF levels but to a lesser extent than TauE14. TauE14 also induces a complete loss of mitochondria from these sLNv projections. However, mitochondria were increased in sLNv cell bodies in TauE14 flies. These results suggest that TauE14 disrupts axonal transport of neuropeptides and mitochondria in circadian pacemaker neurons, providing a mechanism by which Tau can disrupt circadian behavior prior to cell loss.
Comparison of patency of single and sequential radial artery grafting in coronary artery bypass
Authors: Kasahara Hirofumi , Shin Hankei , Takahashi Tatsuo , Murata Satoru , Mori Mitsuharu 
Published: October 2021

Abstract

OBJECTIVES
Sequential radial artery (RA) grafting has the potential to enhance arterial revascularization compared to single grafting. Sequential RA grafting was performed predominantly with a single side-to-side anastomosis. The study aimed to assess if sequential RA grafting improved long-term graft patency compared to single RA grafting. In addition, the anastomotic patencies of side-to-side and end-to-side anastomoses in sequential RA grafting were assessed.

METHODS
Two hundred nineteen patients underwent isolated coronary artery bypass grafting with skeletonized RA conduits between 2005 and 2016. Of these, 208 patients underwent radiological graft assessment; thus, 125 and 83 patients underwent single and sequential RA grafting, respectively. The graft and anastomotic patency rates were estimated using the Kaplan–Meier method.

RESULTS
The median follow-up period was 9.1 years, and the radiological assessment lasted 5.1 years. The overall RA graft patency rates at 1, 5 and 10 years were 99.4%, 92.7% and 88.1%, respectively. The RA graft patency rate for sequential grafting was similar to that for single grafting (88.7% vs 87.4% at 10 years; P = 0.88). In the stratified analysis of anastomotic patency, the patency rate of side-to-side anastomoses of sequential RA grafting was significantly better than that of end-to-side anastomoses (100% vs 88.7% at 10 years; P = 0.01).

CONCLUSIONS
The long-term RA graft patencies of sequential and single grafting were equally high. The anastomotic patency of side-to-side anastomoses of sequential RA grafting was remarkably high. Considering these findings, the RA can be effectively used for multiple arterial coronary revascularizations.
Influence of measurement and sizing techniques in thoracic endovascular aortic repair on outcome in acute complicated type B aortic dissections
Authors: Rychla Miriam , Dueppers Philip , Meuli Lorenz , Rancic Zoran , Menges Anna-Leonie , Kopp Reinhard , Zimmermann Alexander , Reutersberg Benedikt 
Published: October 2021

Abstract

OBJECTIVES
Thoracic endovascular aortic repair (TEVAR) is the first-line therapy in acute complicated type B aortic dissections (cTBAD). Nevertheless, no evidence-based consensus on the optimal measurement technique and sizing for TEVAR in cTBAD exists. The aim was to evaluate how different measurement and sizing techniques for TEVAR affect long-term outcomes.

METHODS
Retrospective analysis investigating the association between sizing and postoperative results after TEVAR in patients with cTBAD, treated between January 2003 and December 2020. Diameter measurements were performed perpendicular to a centreline in pre-interventional Computed tomography angiographies. Oversizing was determined by measuring aortic diameter in zone 2 of the aortic arch in relation to the implanted stent graft, and categorized into 2 sizing groups (≤10% and &gt;10%). The primary outcome was freedom from aortic-related events. Secondary outcomes included mortality and a comparison of 3 alternative measurement techniques considering the estimated pre-dissection diameter.

RESULTS
Fifty-seven patients (median age 69, interquartile range 59.6–78.2 years) were included. Stent graft oversizing by ≤10% showed a trend towards fewer aortic-related events hazard ratio 0.455 (95% confidence interval 0.128–1.624, P = 0.225).
The 3 measurement techniques using the pre-dissection aortic diameter differed by a mean of 1.7–4.0 mm with a variability of up to 8.4 mm. In none of the 57 patients, the same stent graft would have been chosen based on the different measurement techniques using an oversizing ≤10%.

CONCLUSIONS
TEVAR oversizing of ≤10% in patients with cTBAD might reduce aortic-related events up to 50%. Consensus on measurement techniques of the pre-dissection aortic diameter and stent graft sizing is of paramount importance.
Localized malignant pleural mesothelioma of the interlobar fissure mimicking a lung cancer
Authors: Ciriaco Paola , Viscardi Stefano , Filipello Federica , Negri Giampiero 
Published: October 2021

Abstract
We report a case of localized malignant pleural mesothelioma (LMPM) of the interlobar fissure mimicking lung cancer. A 74-year-old woman presented with dyspnoea and a left pleural effusion. Chest computed tomography revealed a bulky mass, arising from the left lower lobe with intense uptake at the 18F-FDG PET. A left lower lobectomy was performed after thoracoscopic pleural biopsies. The final histology revealed an LMPM epithelioid like. We describe the diagnostic and curative path of the disease, speculating on the differential diagnosis and treatment of LMPM.
Regeneration of antigen-specific T cells by using induced pluripotent stem cell (iPSC) technology
Authors: Kawamoto Hiroshi , Masuda Kyoko , Nagano Seiji 
Published: October 2021

Abstract
In currently ongoing adoptive T-cell therapies, T cells collected from the patient are given back to the patient after ex vivo cell activation and expansion. In some cases, T cells are transduced with chimeric antigen receptor (CAR) or T-cell receptor (TCR) genes during the ex vivo culture period. Although such strategies have been shown to be effective in some types of cancer, there remain issues to be solved; these methods (i) are time-consuming, (ii) are costly and (iii) it is difficult to guarantee the quality because the products depend on patient-derived T cells. To address these issues, several groups including ours have developed methods in which cytotoxic cells are mass-produced by using induced pluripotent stem cell (iPSC) technology. For the regeneration of T cells, the basic idea is as follows: iPSCs produced from T cells inherit rearranged TCR genes, and thus all regenerated T cells should express the same TCR. Based on this idea, various types of T cells have been regenerated, including conventional cytotoxic T lymphocytes (CTLs), γδT cells, NKT cells and mucosal-associated invariant T (MAIT) cells. On the other hand, any cytotoxic cells can be used as the base cells into which CAR is introduced, and thus iPSC-derived NK cells have been developed. To apply the iPSC-based cell therapy in an allogeneic setting, the authors’ group developed a method in which non-T-cell-derived iPSCs are transduced with exogenous TCR genes (TCR-iPSC method). This approach is being prepared for a clinical trial to be realized in Kyoto University Hospital, in which acute myeloid leukemia patients will be treated by the regenerated WT1 antigen-specific CTLs.
Bodies Coming Apart and Bodies Becoming Parts: Widening, Deepening, and Embodying Ontological (In)Security in the Context of the COVID-19 Pandemic
Authors: Purnell Kandida 
Published: October 2021

AbstractThis article widens and deepens the notion of ontological security and therefore both the scope of ontological security studies (OSS) within the discipline of international relations (IR) and ontological security theory (OST) writ large by introducing and explaining the implications of (re/dis)embodiment—the continually contested social–political process through which bodies come to be or not be and upon which everybody existentially and ontologically depends. Understood as both a source of and a threat to individual and collective bodies’ ontological security, in this article I explain how taking the process of (re/dis)embodiment into account entails widening and deepening OSS to allow for the consideration and appreciation of how individual and collective bodies are continually, simultaneously, materially, and ideationally contested. As a primarily theoretical contribution, this is done through an interdisciplinary approach bringing Achille Mbembe's necropolitical theory into conversation with Sara Ahmed's theses on willfulness and use and is illustrated through discussions on the body politics of the COVID-19 pandemic. In short, I argue that, under conditions of contemporary global necropolitics, individuals’ ontological security as bodies becomes increasingly threatened according to raced, classed, and gendered local–global hierarchies which determine the reduction and use of individuals to the status of parts within collectives that are themselves embodied and increasingly unfit for the purpose of healthy living, especially in a time of pandemic.
Pathologies of a Different Kind: Dysfunctional Interactions between International Organizations
Authors: Kranke Matthias 
Published: October 2021

AbstractThis article develops the concept of “interorganizational pathologies” as an extension of Michael N. Barnett and Martha Finnemore's work on bureaucratic pathologies. Adopting an open system perspective, I argue that dysfunctional interactions may arise between international organizations (IOs) even when their cooperation is fairly institutionalized. To advance this line of reasoning, I examine interactions between the International Monetary Fund (IMF or Fund) and the World Bank (or Bank). Evidence from more than ninety stakeholder interviews indicates that the interactions have been marked by what Barnett and Finnemore call “insulation.” In particular, two opposing types of interorganizational insulation have been common in the context of Fund–Bank relations: (1) groupthink, or sustained intellectual decoupling by the two IOs from the outside world; and (2) silence, or (temporary) communicative disruption between them. This finding is partly puzzling because while we may expect IMF–World Bank interactions to produce groupthink given the organizations’ highly similar worldviews, we would expect them to prevent silence given established protocols for continuous cross-organizational information sharing. The analysis sheds fresh light on the promises and pitfalls of cogovernance by IOs as key players in regime complexes and transnational networks.
Adoption of Advanced Chemometric Methods for Determination of Pyridoxine HCl, Cyclizine HCl, and Meclizine HCl in the Presence of Related Impurities: A Comparative Study
Authors: Saad Ahmed S , Draz Mohammed E , Naguib Ibrahim A , Zaazaa Hala E , Lashien Adel S , Abdallah Fatma F 
Published: October 2021

AbstractBackgroundNoising is an undesirable phenomenon accompanying the development of widely used chemometric models such as partial least square regression (PLSR) and support vector regression (SVR).ObjectiveOptimizations of these chemometric models by applying orthogonal projection to latent structures (OPLS) as a preprocessing step which is characterized by canceling noise is the purpose of this research study. Additionally, a comprehensive comparative study between the developed methods was undertaken highlighting pros and cons.MethodsOPLS was conducted with PLSR and SVR for quantitative determination of pyridoxine HCl, cyclizine HCl, and meclizine HCl in the presence of their related impurities. The training set was formed from 25 mixtures as there were five mixtures for each compound at each concentration level. Additionally, to check the validity and predictive ability of the developed chemometric models, independent test set mixtures were prepared by repeating the preparation of four mixtures of the training set plus preparation of another four independent mixtures.ResultsUpon application of the OPLS processing method, an upswing of the predictive abilities of PLSR and SVR was found. The root-mean-square error of prediction of the test set was the basic benchmark for comparison.ConclusionThe major finding from the conducted research is that processing with OPLS reinforces the ability of models to anticipate the future samples.HighlightsNovel optimizations of the widely used chemometric models; application of a comparative study between the suggested methods; application of OPLS preprocessing methods; quantitative determination of pyridoxine HCl, cyclizine HCl and meclizine HCl; checking the predictive power of developed chemometric models; analysis of active ingredients in their pharmaceutical dosage forms.
PSXV-17 Dietary postbiotic mediates cytokine gene expression in senior horses
Authors: Norton Sharon A , Adams Amanda A 
Published: October 2021

Abstract
Senior horses often exhibit chronic inflammation and decreased immune responses. Dietary Saccharomyces cerevisiae fermentation product (SCFP; Diamond V, Cedar Rapids, IA) has been shown to affect immune responses in several species. This study aimed to evaluate SCFP-mediated immune function in senior horses. Sixteen horses (24.8 ± 3.0 y; BW = 545.8 ± 61.9 kg) were allotted to two treatments: CON (n = 8; no SCFP supplementation) and SCFP top dressed onto a common concentrate for 56 d (21 g/d; n = 8). Body condition score (BCS), BW, whole blood cytokine (INF-ɣ, TNF-α, IL-1β, IL-6, IL-10, IL-4, IL-8, IL-13, IL-17) and COX-1 and COX-2 gene expression were measured at d 0, 42, 49 and 56. Horses were challenged with a monovalent influenza vaccine at d 42 (MIV; Fluvacc Innovator; Zoetis Animal Health, Parsippany, NJ). Pre-MIV (d 0–42) and post-MIV (d 42–56) responses were analyzed using general mixed model procedures. Pre-MIV, BW tended to increase (P = 0.09) over time. Expression of IL-10 tended to be lower for SCFP than CON (P = 0.09) and IL-13 expression decreased over time (P &lt; 0.05). Post-MIV, BCS linearly increased over time (P = 0.006) while TNF-α tended to increase at d 49 and return to d 42 levels by d 56 (P = 0.06). Both INF-ɣ and IL-10 expression were lower for SCFP vs CON (P &lt; 0.05). Gene expression of COX-2 tended to decrease (P = 0.06) at d 49 and return to the d 42 baseline by d 56. Gene expression of IL-13 tended to decrease with SCFP but increased with CON at d 49 with both returning to d 42 levels by d 56 (P = 0.08). Influenza H1 titers increased over time post-vaccination (P &lt; 0.001) with no difference between treatments. Dietary SCFP may modulate pro-inflammatory and anti-inflammatory cytokine gene expression in senior horses.