bluuebunny/crossref_metadata_2025_split huggingface dataset:
Correlation of Alberta Stroke Program Early Computed Tomography Score With Computed Tomography Perfusion Core in Large Vessel Occlusion in Delayed Time Windows
Authors: Voleti Sriharsha , Vidovich Johnathan , Corcoran Brendan , Zhang Bin , Khandwala Vivek , Mistry Eva A. , Khatri Pooja , Tomsick Thomas , Vagal Achala 
Published: January 2021

Background and Purpose:
The Alberta Stroke Program Early Computed Tomography (CT) Score (ASPECTS) and CT perfusion (CTP) are commonly used to predict the ischemic core in acute ischemic strokes. CT angiography source images (CTA-SI) can also provide additional information to identify the extent of ischemia. Our objective was to investigate the correlation of noncontrast CT (NCCT) ASPECTS and CTA-SI ASPECTS with CTP core volumes.

Methods:
We utilized a single institutional, retrospective registry of consecutive patients with acute ischemic stroke with large vessel occlusion between May 2016 and May 2018. We graded ASPECTS both on baseline NCCT and CTA-SI and measured CTP core using automated RAPID software (cerebral blood flow <30%). We used Spearman’s correlation coefficients to evaluate the correlation between continuous variables.

Results:

A total of 52 patients fit the inclusion criteria of large vessel occlusion in 6 to 24 hours and baseline imaging work up of NCCT, CTA, and CTP. The median age was 63 (interquartile range=53.5–75) and 38.46% were female. The median NCCT ASPECTS was 7 (interquartile range=6–9), CTA-SI ASPECTS was 5 (interquartile range=4–7), and CTP core was 14.5 mL (interquartile range=0–46 mL). There was a moderate correlation between NCCT ASPECTS and CTP core (r
s
=−0.55,
P
<0.0001) and between CTA-SI ASPECTS and CTP core (r
s
=−0.50,
P
=0.0002). The optimal NCCT ASPECTS cutoff score to detect CTP core ≤70 mL was ≥6 (sensitivity, 0.84; specificity, 0.57; positive predictive value, 0.93; negative predictive value, 0.36) and the optimal CTA-SI ASPECTS was ≥5 (sensitivity, 0.76; specificity, 0.71; positive predictive value, 0.94; negative predictive value, 0.31).

Conclusions:
There was a moderate correlation between NCCT and CTA-SI ASPECTS in predicting CTP defined ischemic core in delayed time windows. Further studies are needed to determine if NCCT and CTA imaging could be used for image-based patient selection when CTP imaging is not available.
Average Lifespan Shortened due to Stroke in Canada
Authors: Pham Truong-Minh , Thanh Nguyen Xuan , Wasylak Tracy , Hill Michael D. , Jeerakathil Thomas , Sikdar Khokan C. , Kaposhi Bethany , Shack Lorraine , Cheung Winson Y. 
Published: January 2021

Background and Purpose:
There are challenges in comparability when using existing life lost measures to examine long-term trends in premature mortality. To address this important issue, we have developed a novel measure termed average lifespan shortened (ALSS). In the present study, we used the ALSS measure to describe temporal changes in premature mortality due to stroke in the Canadian population from 1990 to 2015.

Methods:
Mortality data for stroke were obtained from the World Health Organization mortality database. Years of life lost was calculated using Canadian life tables. ALSS was calculated as the ratio of years of life lost in relation to the expected lifespan.

Results:
Over a 25-year timeframe, the age-standardized rates adjusted to the World Standard Population for deaths from all strokes and stroke types substantially decreased in both sexes. The ALSS measure indicated that men who died of stroke lost 12.1% of their lifespan in 1990 and 11.4% in 2015, whereas these values among women were 11.1% and 10.0%, respectively. Patients with subarachnoid hemorrhagic stroke lost the largest portion whereby both sexes lost about one-third of their lifespan in 1990 and one-fourth in 2015. Men with intracerebral hemorrhagic stroke lost around 18% of their lifespan in 1990 and 14% in 2015 as compared to women who lost about 16% and 12% over the same timeframe. The loss of lifespan for patients with ischemic stroke and other stroke types combined was relatively stable at about 10% throughout the study period.

Conclusions:
Our study demonstrated a modest improvement in lifespan among patients with stroke in Canada between 1990 and 2015. Our novel ALSS measure provides intuitive interpretation of temporal changes in lifespan among patients with stroke and helps to enhance our understanding of the burden of strokes in the Canadian population.
Spider monkey use of natural and artificial terrestrial water sources in Calakmul, Mexico
Authors: Delgado-Martínez Carlos M. , Spaan Denise , Contreras-Moreno Fernando M. , Simá-Pantí David E. , Mendoza Eduardo 
Published: January 2021

Abstract
Geoffroy’s spider monkeys (Ateles geoffroyi) satisfy their need for water mainly from food items and arboreal water sources, but climate change and other anthropogenic impacts can drive a more frequent use of ground water by this arboreal species. Here, we report 25 events of use of natural and artificial terrestrial water bodies by spider monkeys recorded using camera-trapping in the Calakmul region, Mexico. Interestingly, these events occurred during the rainy season in locations where we did not record large predators and in most cases involved adult male individuals. These records suggest that habitat disruptions such as those associated with changing weather patterns and selective logging of large trees holding water reservoirs can lead to a greater use of terrestrial water sources by spider monkeys.
Problem-solving performance in wild Steller’s jays using a string-pulling task
Authors: Harvey Derek P. , Black Jeffrey M. 
Published: January 2021

Abstract
Animals that exploit resources from human-modified environments may encounter unique problems when searching for food. Pulling a string tied to a food reward (string-pulling task) is one of the most widespread methods of testing a species’ problem-solving performance in non-human animals. Performance in problem-solving tasks may be influenced by an individual’s characteristics and social interactions, especially in its natural habitat. We examined problem solving by free-ranging Steller’s jays (Cyanocitta stelleri) when extracting food from a string-pulling task presented in their natural habitat. During the study, seven of 50 jays successfully solved the task on their first to eighteenth experimental opportunity; solvers differed from nonsolvers by showing higher levels of persistence by pulling the string in more trials. Of the successful jays, five birds solved without observing others, while two birds were present during successful trials and subsequently completed the task. All seven jays demonstrated improvement in the task by using less string pulls over additional successful trials. Nineteen other jays in the population interacted with the apparatus and pulled the string, but not enough to acquire the food. These 19 jays were significantly bolder (shorter latencies to approach), more explorative (contacted more parts of the apparatus), and had observed solvers more than the 24 individuals that did not pull the string. These results indicate a broad spectrum of individual differences in propensity for solving novel tasks in our population of Steller’s jays.
Aerobic exercise improves VCI through circRIMS2/miR-186/BDNF-mediated neuronal apoptosis
Authors: Niu Yali , Wan Chunxiao , Zhang Jing , Zhang Shu , Zhao Zilong , Zhu Leshan , Wang Xiaodong , Ren Xiaodong , Wang Jing , Lei Ping 
Published: January 2021

Abstract
Background
Vascular cognitive impairment (VCI) is a common cognitive disorder caused by cerebrovascular disease, ranging from mild cognitive impairment to dementia. Studies have shown that aerobic exercise might alleviate the pathological development of VCI, and our previous study observed that aerobic exercise could alleviate VCI through NF-κB/miR-503/BDNF pathway. However, there are few studies on the mechanism. Therefore, it is of great significance to fill the gaps in the mechanism for the early diagnosis of VCI and the clinical prevention and treatment of vascular dementia.

Methods
CircRNA microarray analysis and quantitative real-time PCR were used to detect the expression of circRNA regulating synaptic be exocytosis 2 (RIMS2) (circRIMS2). Cell apoptosis was determined by TdT-mediated dUTP nick-end labeling (TUNEL) assay. The dual-luciferase reporter assay was performed to verify the interaction between circRIMS2 and miR-186, as well as miR-186 and BDNF. RNA pull-down assay detected the binding between circRIMS2 and miR-186. A VCI mouse model was established by repeated ligation of bilateral common carotid arteries (2VO). The lentiviral interfering vector was injected into the VCI mice through the lateral ventricle. The mice in the aerobic exercise group performed 30 min (12 m/min) running for 5 days a week. A Morris water maze test was performed after 4 weeks.

Results
The expression of circRIMS2 and BDNF in the serum of VCI patients was significantly reduced, miR-186 expression was increased, and the expression of circRIMS2 was increased in the 2VO group of mice undergoing aerobic exercise. The expression levels of circRIMS2 and BDNF in the oxygen and glucose deprivation-treated (OGD-treated) cells were decreased, the miR-186 expression and cell apoptosis were increased, while the effect was weakened after transfection with the lentiviral vector pLO-ciR-RIMS2. CircRIMS2 could bind to miR-186, and after interference with circRIMS2 in HT22 cells, the expression of miR-186 was increased. Besides, miR-186 could bind to BDNF, and BDNF expression was decreased because of the overexpression of miR-186 in HT22 cells. The expression level of BDNF in the pLO-ciR-RIMS2 group was increased, and apoptosis was decreased, but the miR-186 mimic weakened the effect of pLO-ciR-RIMS2. Aerobic exercise could shorten the average time that mice reached the platform in the Morris water maze, increase the expression level of circRIMS2 and BDNF, reduce miR-186 expression, and inhibit neuronal apoptosis. However, the interference with circRIMS2 weakened this effect.

Conclusion
The expression of circRIMS2 was down-regulated in VCI and aerobic exercise reduced neuronal apoptosis, and circRIMS2 improved VCI through the circRIMS2/miR-186/BDNF axis.
Wind-induced vibration performance of early Chinese hall-style timber buildings
Authors: Han Yidan , Chun Qing , Jin Hui 
Published: January 2021

AbstractTraditional Chinese timber buildings are lightweight, flexible, and quite vulnerable to wind. This paper aims to present a methodology for evaluating the wind-induced vibration performance of early Chinese hall-style timber buildings based on numerical simulations. First, the architectural and structural characteristics of early Chinese hall-style timber buildings were presented and analyzed. It is found that the main hall of the Tianning Temple can be considered a representative to carry out the research. Second, the wind pressure coefficients of the main hall were determined via computational fluid dynamics (CFD) simulation. Finally, the time-series wind pressures were generated, and the wind-induced vibration performance was analyzed by the finite-element method (FEM). The CFD simulation results show that the eave corners bear the maximum wind suction, and the windward pediment bears the maximum wind pressure. The FEM analysis has revealed that the lateral structural stiffness in the depth direction is smaller. Under the depth-direction wind load, most of the large displacement occurs intensively at the middle part of the windward frames. The structural response is dominated mainly by the top three vibration modes. The time-series wind vibration coefficients are larger than the code values by a factor of 1.39–1.82. This study can provide a reference for evaluating the wind-induced vibration performance of early Chinese hall-style timber buildings.
Enhanced UV Emission from ZnO on Silver Nanoparticle Arrays by the Surface Plasmon Resonance Effect
Authors: Wang Xiao , Ye Qiong , Bai Li-Hua , Su Xi , Wang Ting-Ting , Peng Tao-Wei , Zhai Xiao-Qi , Huo Yi , Wu Hao , Liu Chang , Bu Yu-Yu , Ma Xiao-Hua , Hao Yue , Ao Jin-Ping 
Published: January 2021

AbstractPeriodical silver nanoparticle (NP) arrays were fabricated by magnetron sputtering method with anodic aluminum oxide templates to enhance the UV light emission from ZnO by the surface plasmon resonance effect. Theoretical simulations indicated that the surface plasmon resonance wavelength depended on the diameter and space of Ag NP arrays. By introducing Ag NP arrays with the diameter of 40 nm and space of 100 nm, the photoluminescence intensity of the near band-edge emission from ZnO was twofold enhanced. Time-resolved photoluminescence measurement and energy band analysis indicated that the UV light emission enhancement was attributed to the coupling between the surface plasmons in Ag NP arrays and the excitons in ZnO with the improved spontaneous emission rate and enhanced local electromagnetic fields.
Evaluation of the potential of Pap test fluid and cervical swabs to serve as clinical diagnostic biospecimens for the detection of ovarian cancer by mass spectrometry-based proteomics
Authors: Boylan Kristin L. M. , Afiuni-Zadeh Somaieh , Geller Melissa A. , Argenta Peter A. , Griffin Timothy J. , Skubitz Amy P. N. 
Published: January 2021

Abstract
Background
The purpose of this study was to determine whether the residual fixative from a liquid-based Pap test or a swab of the cervix contained proteins that were also found in the primary tumor of a woman with high grade serous ovarian cancer. This study is the first step in determining the feasibility of using the liquid-based Pap test or a cervical swab for the detection of ovarian cancer protein biomarkers.

Methods
Proteins were concentrated by acetone precipitation from the cell-free supernatant of the liquid-based Pap test fixative or eluted from the cervical swab. Protein was also extracted from the patient’s tumor tissue. The protein samples were digested into peptides with trypsin, then the peptides were run on 2D-liquid chromatography mass spectrometry (2D-LCMS). The data was searched against a human protein database for the identification of peptides and proteins in each biospecimen. The proteins that were identified were classified for cellular localization and molecular function by bioinformatics integration.

Results
We identified almost 5000 proteins total in the three matched biospecimens. More than 2000 proteins were expressed in each of the three biospecimens, including several known ovarian cancer biomarkers such as CA125, HE4, and mesothelin. By Scaffold analysis of the protein Gene Ontology categories and functional analysis using PANTHER, the proteins were classified by cellular localization and molecular function, demonstrating that the Pap test fluid and cervical swab proteins are similar to each other, and also to the tumor extract.

Conclusions
Our results suggest that Pap test fixatives and cervical swabs are a rich source of tumor-specific biomarkers for ovarian cancer, which could be developed as a test for ovarian cancer detection.
Early childhood screen time as a predictor of emotional and behavioral problems in children at 4 years: a birth cohort study in China
Authors: Liu Wenwen , Wu Xiaoyan , Huang Kun , Yan Shuangqin , Ma Liya , Cao Hui , Gan Hong , Tao Fangbiao 
Published: January 2021

Abstract
Background
Previous studies have suggested that screen time (ST) has a negative effect on children’s emotional and behavioral health, but there are few longitudinal studies that have been conducted with infants and toddlers. This study sought to examine the effect of ST in early childhood on emotional and behavioral problems in children aged 4 years, based on a birth cohort study in China.

Methods
A total of 2492 children aged 4 years were enrolled in this study. The parents and guardians of each child completed a questionnaire that included items eliciting information on children’s birth information, socio-demographic information at baseline, and ST at each follow-up. Emotional and behavioral problems were assessed using the Strengths and Difficulties Questionnaire (SDQ) at 4 years of age. Multivariate logistic analysis was used to explore the effects of ST on emotional and behavioral problems.

Results
The percentages of children with ST > 0 h/day at age 0.5 years, ST > 2 h/day at age 2.5 years, and ST > 2 h/day at age 4 years were 45.7, 55.5, and 34.5% respectively. The prevalence of emotional and behavioral problems was 10.8%. ST at 6 months was a risk factor for emotional symptoms and hyperactivity at the age of 4 years. ST at age 2.5 years was a risk factor for hyperactivity at the age of 4 years. However, ST at age 4 years was a risk factor for total difficulties, conduct problems, peer problems, hyperactivity, and prosocial behavior.

Conclusions
Higher ST exposure at early childhood is associated with later emotional and behavioral problems. In particular, sustained high ST exposure is a risk factor for behavioral problems. These findings suggested the importance of controlling ST to prevent the occurrence of emotional and behavioral problems in the early years.
Histology of variants of urothelial carcinoma of the bladder: a Moroccan series of 39 cases
Authors: Saouli Amine , Karmouni Tarik , El Khader Khalid , Koutani Abdellatif , Andaloussi Ahmed Iben Attya 
Published: January 2021

Background
The variant of urothelial carcinoma (UCV) is a rare and aggressive histological entity; its therapeutic management remains debated.

Methods
Between January 2017 and October 2019, 39 patients were diagnosed retrospectively with urothelial carcinoma with a histological variant. The aim of this study was to analyze the clinico-pathological and therapeutic aspects of these variants to better understand this invasive disease.

Results
In 39 cases, 36 (92%) were men and 3 (8%) were women; the average age was 62 years; 54% of patients had a ≥ cT3 stage with clinical lymph node involvement in 28% and 23% had distant metastases, 36% of patients were treated with RC and 54% by other therapeutic and 10% refused RC. No patient received neoadjuvant chemotherapy. Lymph node involvement was 28.5%. The two predominant histological types were squamous and micropapillary differentiation between the operating piece and bladder biopsy 28.5%, 30% and 21.5% and 13%, respectively. The overall survival at 20 months was 70 and 40% for RC and the other therapeutic (TURB alone or a TURB with an adjuvant), respectively. There was no significant difference in survival between the two groups (p = 0.27).

Conclusion
UCVs are heterogeneous groups of tumors which are increasingly identified due to their distinct morphological characteristics with variable clinical prognosis. Therapeutic management is not in favor of cystectomy in our study. However, a prospective study using a larger cohort is still necessary to validate our results.
A Novel PCR Method for Detecting ACE Gene Insertion/Deletion Polymorphisms and its Clinical Application
Authors: Yang Xue-min , Liang Jian-ping , Huang Xiao-juan , Wang Xiang-rong , Sun Yang , Dong Chen , Cui Ya-li , Hui Wen-li 
Published: January 2021

Abstract
Background
Angiotensin-converting enzyme (ACE) plays a major role in blood pressure regulation and cardiovascular homeostasis. The wide distribution and multifunctional properties of ACE suggest it’s involvement in various pathophysiological conditions.

Results
In this study, a novel visual detection method for ACE I/D polymorphisms was designed by integrating direct PCR without the need for DNA extraction using gold magnetic nanoparticles (GMNPs)-based lateral flow assay (LFA) biosensor. The entire detection procedure could enable the genotyping of clinical samples in about 80 min. The detection limit was 0.75 ng and results could be obtained in 5 min using the LFA device. Three hundred peripheral blood samples were analyzed using the direct PCR-LFA system and then verified by sequencing to determine accuracy and repeatability. A clinical preliminary study was then performed to analyze a total of 633 clinical samples.

Conclusions
After grouping based on age, we found a significant difference between the genotypes and the age of patients in the CHD group. The introduction of this method into clinical practice may be helpful for the diagnosis of diseases caused by large fragment gene insertions/deletions.
Temporal changes in DNA methylation and RNA expression in a small song bird: within- and between-tissue comparisons
Authors: Lindner Melanie , Verhagen Irene , Viitaniemi Heidi M. , Laine Veronika N. , Visser Marcel E. , Husby Arild , van Oers Kees 
Published: January 2021

AbstractBackgroundDNA methylation is likely a key mechanism regulating changes in gene transcription in traits that show temporal fluctuations in response to environmental conditions. To understand the transcriptional role of DNA methylation we need simultaneous within-individual assessment of methylation changes and gene expression changes over time. Within-individual repeated sampling of tissues, which are essential for trait expression is, however, unfeasible (e.g. specific brain regions, liver and ovary for reproductive timing). Here, we explore to what extend between-individual changes in DNA methylation in a tissue accessible for repeated sampling (red blood cells (RBCs)) reflect such patterns in a tissue unavailable for repeated sampling (liver) and how these DNA methylation patterns are associated with gene expression in such inaccessible tissues (hypothalamus, ovary and liver). For this, 18 great tit (Parus major) females were sacrificed at three time points (n = 6 per time point) throughout the pre-laying and egg-laying period and their blood, hypothalamus, ovary and liver were sampled.ResultsWe simultaneously assessed DNA methylation changes (via reduced representation bisulfite sequencing) and changes in gene expression (via RNA-seq and qPCR) over time. In general, we found a positive correlation between changes in CpG site methylation in RBCs and liver across timepoints. For CpG sites in close proximity to the transcription start site, an increase in RBC methylation over time was associated with a decrease in the expression of the associated gene in the ovary. In contrast, no such association with gene expression was found for CpG site methylation within the gene body or the 10 kb up- and downstream regions adjacent to the gene body.ConclusionTemporal changes in DNA methylation are largely tissue-general, indicating that changes in RBC methylation can reflect changes in DNA methylation in other, often less accessible, tissues such as the liver in our case. However, associations between temporal changes in DNA methylation with changes in gene expression are mostly tissue- and genomic location-dependent. The observation that temporal changes in DNA methylation within RBCs can relate to changes in gene expression in less accessible tissues is important for a better understanding of how environmental conditions shape traits that temporally change in expression in wild populations.