bluuebunny/crossref_metadata_2025_split huggingface dataset:
Generating aptamers towards human sperm cells using massively parallel sequencing
Authors: Gooch James , Tungsirisurp Sireethorn , Costanzo Hayley , Napier Richard , Frascione Nunzianda 
Published: August 2021

AbstractDetermining the presence of sperm cells on an item or swab is often a crucial component of sexual offence investigation. However, traditional histological staining techniques used for the morphological identification of spermatozoa lack both specificity and sensitivity, making analysis a complex and time-consuming process. New methods for the detection of sperm cells based on aptamer recognition may be able to overcome these issues. In this work, we present the selection of ssDNA aptamers against human sperm cells using Cell-SELEX and massively parallel sequencing technologies. A total of 14 rounds of selection were performed following a modified Cell-SELEX protocol, which included additional steps for the isolation of spermatozoa from seminal fluid. Massively parallel sequencing using the Illumina Miseq platform was conducted on enriched aptamer pools to elucidate the structure of potential binders. A custom bioinformatics pipeline was also developed using Galaxy for the automated processing of sequencing datasets. This data revealed several promising aptamer candidates, which were shown to selectively bind sperm cells through both microscale thermophoresis and enzyme-linked oligonucleotide assays. These aptamers have the potential to increase the efficiency of sexual offence casework by facilitating sperm detection.
Graphical abstract
A randomized, double-blind, single-dose study (LAVENDER) to assess the safety, tolerability, pharmacokinetics, and immunogenicity of a combined infusion of ABP 980 and pertuzumab in healthy subjects
Authors: Hanes Vladimir , Chow Vincent , Stewart Tina , Puri Adeep 
Published: August 2021

Abstract
Purpose
ABP 980 (KANJINTI™) is a biosimilar to reference product HERCEPTIN® (trastuzumab RP). The goal of this study was to characterize the safety, tolerability, and immunogenicity of ABP 980 plus pertuzumab (PERJETA®) when co-administered in a single infusion bag in healthy subjects.

Methods
This randomized, double-blind, single-dose, 2-arm, parallel-group study (LAVENDER Study) evaluated an intravenous (IV) infusion of ABP 980 (6 mg/kg) plus pertuzumab (420 mg) combined in a single infusion bag relative to an IV infusion of trastuzumab RP (6 mg/kg) plus pertuzumab (420 mg) combined in a single infusion bag given over 60 min. The subjects were followed for 92 days post dosing.

Results
A total of 42 subjects were enrolled in the study and treated with investigational product. Due to an operational issue during dosing, the first 6 subjects enrolled in the study were replaced. A total of 36 randomized subjects, n = 18 for ABP 980 plus pertuzumab and n = 18 for trastuzumab RP plus pertuzumab, were treated. Resulting serum concentrations of ABP 980 and trastuzumab RP were similar. There were no serious adverse events, no deaths, and no cardiac disorders during the study. No subject developed anti-drug antibodies throughout the study.

Conclusions
This study demonstrated the safety and tolerability of ABP 980 and pertuzumab admixture in a single infusion bag. The safety profiles and pharmacokinetic parameters of ABP 980 and pertuzumab were consistent with what is known for trastuzumab RP and pertuzumab.

Clinical trial listing
EudraCT 2018-002903-33.
Prävalenz kraniomandibulärer Dysfunktionen bei Senioren – symptombezogene Analysen bei jüngeren und älteren Senioren
Authors: Rauch Angelika , Angrik Cäcilie , Zenthöfer Andreas , Weber Sophia , Hahnel Sebastian , Nitschke Ina , Schierz Oliver 
Published: August 2021

ZusammenfassungHintergrundKraniomandibuläre Dysfunktionen (CMD) können auch im hohen Alter auftreten. Die Prävalenz von CMD-Symptomen bei Senioren wurde bisher nur wenig untersucht.Ziel der ArbeitZiel dieser Untersuchung war es, die Prävalenz von Symptomen einer CMD bei Senioren nach Befunderhebung mit den Research Diagnostic Criteria for Temporomandibular Disorders (RDC/TMD) zu bestimmen. Dabei sollten die Prävalenzwerte von jüngeren (60 bis 74 Jahren) und älteren (≥ 75 Jahre) Senioren verglichen werden.Material und MethodenIm Rahmen der Interdisziplinären Längsschnittstudie des Erwachsenenalters (ILSE) wurden Probanden nach repräsentativen Gesichtspunkten rekrutiert. Während der vierten Nachverfolgungswelle im Zeitraum von 2014 bis 2016 im Bereich des Studienzentrums Leipzig wurden die Probanden auf das Vorliegen von anamnestischen und klinischen CMD-Symptomen untersucht.ErgebnisseAnamnestische CMD-Symptome bei Senioren (n = 192) waren v. a. durch Schmerzen im Gesichtsbereich (13,0 %) gekennzeichnet. Das häufigste klinische CMD-Symptom waren Kiefergelenkgeräusche mit einer Prävalenz bis zu 35,5 %. Frauen gaben anamnestisch häufiger Kopfschmerzen/Migräne an. Kiefergelenkgeräusche und eine limitierte Mundöffnung wurden klinisch häufiger bei weiblichen Teilnehmenden beobachtet. Statistisch signifikante Unterschiede zeigten sich bei dem Vergleich von jüngeren und älteren Senioren hinsichtlich der Prävalenz von Kopfschmerzen/Migräne, jedoch nicht bei klinischen Symptomen.SchlussfolgerungAnamnestisch werden Gesichtsschmerzen von 13,0 % der Senioren angegeben. Kiefergelenkgeräusche werden bei jedem dritten Älteren klinisch beobachtet. CMD-Symptome scheinen bei jüngeren und älteren Senioren im ähnlichen Maße ausgeprägt zu sein.
Recurrent fusions in PLAGL1 define a distinct subset of pediatric-type supratentorial neuroepithelial tumors
Authors: Sievers Philipp , Henneken Sophie C. , Blume Christina , Sill Martin , Schrimpf Daniel , Stichel Damian , Okonechnikov Konstantin , Reuss David E. , Benzel Julia , Maaß Kendra K. , Kool Marcel , Sturm Dominik , Zheng Tuyu , Ghasemi David R. , Kohlhof-Meinecke Patricia , Cruz Ofelia , Suñol Mariona , Lavarino Cinzia , Ruf Viktoria , Boldt Henning B. , Pagès Mélanie , Pouget Celso , Schweizer Leonille , Kranendonk Mariëtte E. G. , Akhtar Noreen , Bunkowski Stephanie , Stadelmann Christine , Schüller Ulrich , Mueller Wolf C. , Dohmen Hildegard , Acker Till , Harter Patrick N. , Mawrin Christian , Beschorner Rudi , Brandner Sebastian , Snuderl Matija , Abdullaev Zied , Aldape Kenneth , Gilbert Mark R. , Armstrong Terri S. , Ellison David W. , Capper David , Ichimura Koichi , Reifenberger Guido , Grundy Richard G. , Jabado Nada , Krskova Lenka , Zapotocky Michal , Vicha Ales , Varlet Pascale , Wesseling Pieter , Rutkowski Stefan , Korshunov Andrey , Wick Wolfgang , Pfister Stefan M. , Jones David T. W. , von Deimling Andreas , Pajtler Kristian W. , Sahm Felix 
Published: August 2021

AbstractEpendymomas encompass a heterogeneous group of central nervous system (CNS) neoplasms that occur along the entire neuroaxis. In recent years, extensive (epi-)genomic profiling efforts have identified several molecular groups of ependymoma that are characterized by distinct molecular alterations and/or patterns. Based on unsupervised visualization of a large cohort of genome-wide DNA methylation data, we identified a highly distinct group of pediatric-type tumors (n = 40) forming a cluster separate from all established CNS tumor types, of which a high proportion were histopathologically diagnosed as ependymoma. RNA sequencing revealed recurrent fusions involving the pleomorphic adenoma gene-like 1 (PLAGL1) gene in 19 of 20 of the samples analyzed, with the most common fusion being EWSR1:PLAGL1 (n = 13). Five tumors showed a PLAGL1:FOXO1 fusion and one a PLAGL1:EP300 fusion. High transcript levels of PLAGL1 were noted in these tumors, with concurrent overexpression of the imprinted genes H19 and IGF2, which are regulated by PLAGL1. Histopathological review of cases with sufficient material (n = 16) demonstrated a broad morphological spectrum of tumors with predominant ependymoma-like features. Immunohistochemically, tumors were GFAP positive and OLIG2- and SOX10 negative. In 3/16 of the cases, a dot-like positivity for EMA was detected. All tumors in our series were located in the supratentorial compartment. Median age of the patients at the time of diagnosis was 6.2 years. Median progression-free survival was 35 months (for 11 patients with data available). In summary, our findings suggest the existence of a novel group of supratentorial neuroepithelial tumors that are characterized by recurrent PLAGL1 fusions and enriched for pediatric patients.
Cross-cultural adaptation and validation of the 22-item sinonasal outcome test (SNOT-22) in German-speaking patients: a prospective, multicenter cohort study
Authors: Albrecht Tobias , Beule Achim Georg , Hildenbrand Tanja , Gerstacker Kathrin , Praetorius Mark , Rudack Claudia , Baumann Ingo 
Published: August 2021

Abstract
Purpose
Chronic rhinosinusitis (CRS) is a common condition associated with a significant reduction of the health-related quality of life. One of the most widely used assessment tools in CRS is the disease-specific, health-related questionnaire SNOT-22. The aim of this study was to translate and validate the SNOT-22 into the German language.

Methods
The questionnaire was translated using the forward–backward translation technique. After the translation its reliability, validity, and sensitivity were evaluated. For this purpose, the questionnaire was completed by patients diagnosed with CRS before, 3 months and 1 year after endoscopic sinus surgery and by healthy individuals as controls at three university hospitals in Germany. The individual scores of the questionnaire before surgery was correlated with the Lund–Mackay score as well as a global disease-specific question.

Results
A total of 139 CRS patients and 31 healthy individuals participated in the study. Internal consistency at all timepoints was very good, with Cronbach’s alpha scores of 0.897, 0.941, and 0.945. The questionnaire was able to discriminate between CRS patients and control subjects (p < 0.0001) and scores improved significantly 3 month and 1 year after sinus surgery (p < 0.0001), indicating a good test–retest reliability, validity, and responsiveness. A significant correlation to the single global disease-specific question could be found (p < 0.0001), but no correlation with the Lund–Mackay score.

Conclusion
The German Version of the SNOT-22 is a reliable, valid, and sensitive instrument for measuring health-related quality of life in patients with CRS. It can be recommended for clinical practice and outcome research for German-speaking patients.
Maternal SARS-CoV-2 infection during pregnancy: possible impact on the infant
Authors: Morhart Patrick , Mardin Christian , Rauh Manfred , Jüngert Jörg , Hammersen Johanna , Kehl Sven , Schuh Wolfgang , Maier-Wohlfart Sigrun , Hermes Katharina , Neubert Antje , Schneider Michael , Hein Alexander , Woelfle Joachim , Schneider Holm 
Published: August 2021

AbstractThe risk and potential consequences of mother-to-child transmission of severe acute respiratory syndrome-coronavirus type 2 (SARS-CoV-2) during pregnancy are still a matter of debate. We studied the impact of SARS-CoV-2 infection on 56 complete households, including 27 newborns whose mothers were pregnant when exposed to the virus. Two PCR-confirmed perinatal SARS-CoV-2 transmissions with mild symptoms in affected neonates were recorded. In addition, we observed a severe eye malformation (unilateral microphthalmia, optic nerve hypoplasia, and congenital retinopathy) associated with maternal SARS-CoV-2 infection in weeks 5 and 6 of embryonic development. This embryopathy could not be explained by other infectious agents, genetic factors, drug use, or maternal disease during pregnancy. Eight other women with a history of SARS-CoV-2 infection prior to gestational week 12, however, delivered healthy infants.Conclusion: The repeated occurrence of mother-to-child transmission in our cohort with risks that remain incompletely understood, such as long-term effects and the possibility of an embryopathy, should sensitize researchers and stimulate further studies as well as support COVID-19 vaccination recommendations for pregnant women. Trial registration number: NCT04741412. Date of registration: November 18, 2020

What is Known:•Materno-fetal transmission of severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2) during pregnancy has rarely been reported so far, but was demonstrated in isolated cases.

What is New:•In a study of complete households with documented SARS-CoV-2 infection, including a cohort of pregnant women, we observed perinatal coronavirus transmission at a higher frequency than expected.•We also describe a newborn boy with an eye malformation reminiscent of rubella embryopathy but associated with early gestation SARS-CoV-2 infection of his mother.•A coronavirus-related embryopathy, reported here for the first time, is a finding that requires further investigation.
Opinion: What do rescue experiments with heterologous proteins tell us and what not?
Authors: Streit Adrian 
Published: August 2021

AbstractThe recent progress in sequencing technology allowed the compilation of gene lists for a large number of organisms, though many of these organisms are hardly experimentally tractable when compared with well-established model organisms. One popular approach to further characterize genes identified in a poorly tractable organism is to express these genes in a model organism, and then ask what the protein does in this system or if the gene is capable of replacing the homologous endogenous one when the latter is mutated. While this is a valid approach for certain questions, I argue that the results of such experiments are frequently wrongly interpreted. If, for example, a gene from a parasitic nematode is capable of replacing its homologous gene in the model nematode Caenorhabditis elegans, it is often concluded that the gene is most likely involved in the same biological process in its own organism as the C. elegans gene is in C. elegans. This conclusion is not valid. All this experiment tells us is that the chemical properties of the parasite protein are similar enough to the ones of the C. elegans protein that it can perform the function of the C. elegans protein in C. elegans. Here I discuss this misconception and illustrate it using the analog of similar electric switches (components) controlling various devices (processes).
Shade affects magnitude and tactics of juvenile Chinook salmon antipredator behavior in the migration corridor
Authors: Sabal Megan C. , Workman Michelle L. , Merz Joseph E. , Palkovacs Eric P. 
Published: August 2021

AbstractEnvironmental conditions strongly affect antipredator behaviors; however, it is less known how migrating prey adjust antipredator behavior in migration corridors, in part, because active migrants are difficult to observe and study. Migrants are vulnerable and encounter many predators in the corridor, and their propensity to travel towards their destination ties antipredator behavior with movement. We evaluated how environmental risk cues in the migration corridor including in-water habitat structure (present, absent) and overhead shade (sun, shade), and salmon origin (hatchery, wild) affected how juvenile Chinook salmon (Oncorhynchus tshawytscha) reacted to a live predator. We measured how salmon react to predation risk as the difference in time to swim downstream through a 9.1-m long field enclosure with or without a live predatory largemouth bass (Micropterus salmoides). Shade significantly modified the reaction to the predator, and it did so in two ways. First, the magnitude of antipredator behavior was larger in shade compared to direct sun, which suggests salmon perceived shade to be a riskier environment than sun. Second, the escape tactic also varied; salmon slowed down to be cautious in shade and sped up in sun. Structure did not significantly affect behavior and hatchery and wild salmon behaved similarly. Our study suggests that environmental risk cues can shape the magnitude and tactics of how migrants react to predation risk and illustrates how these responses relate to movement with potential to scale up and affect migration patterns.
A unified modelling and simulation for coupled anomalous transport in porous media and its finite element implementation
Authors: Barrera O. 
Published: August 2021

AbstractThis paper presents an unified mathematical and computational framework for mechanics-coupled “anomalous” transport phenomena in porous media. The anomalous diffusion is mainly due to variable fluid flow rates caused by spatially and temporally varying permeability. This type of behaviour is described by a fractional pore pressure diffusion equation. The diffusion transient phenomena is significantly affected by the order of the fractional operators. In order to solve 3D consolidation problems of large scale structures, the fractional pore pressure diffusion equation is implemented in a finite element framework adopting the discretised formulation of fractional derivatives given by Grunwald–Letnikov (GL). Here the fractional pore pressure diffusion equation is implemented in the commercial software Abaqus through an open-source UMATHT subroutine. The similarity between pore pressure, heat and hydrogen transport is also discussed in order to show that it is possible to use the coupled thermal-stress analysis to solve fractional consolidation problems.
Is this love? Sex differences in dog-owner attachment behavior suggest similarities with adult human bonds
Authors: D’Aniello Biagio , Scandurra Anna , Pinelli Claudia , Marinelli Lieta , Mongillo Paolo 
Published: August 2021

AbstractSex differences in the behavioral responses of Labrador Retriever dogs in the Strange Situation Test were explored. Behaviors expressed by dogs during seven 3-min episodes were analyzed through a Principal Component Analysis (PCA). The scores of factors obtained were analyzed with a Generalized Linear Mixed Model to reveal the effects of the dog’s sex and age and the owner’s sex. In Episode 1 (dog and owner) and 5 (dog alone), the PCA identified three and two factors, respectively, which overall explained 68.7% and 59.8% of the variance, with no effect of sex. In Episodes 2 (dog, owner, and stranger), 3 and 6 (dog and stranger), and 4 and 7 (dog and owner), the PCA identified four factors, which overall explained 51.0% of the variance. Effects of sex were found on: Factor 1 (distress), with lower scores obtained by females in Episode 2 and higher in Episode 3; Factor 2 (sociability), which was overall higher in females; Factor 3 (separation-distress), with females, but not males, obtaining higher scores when left with the stranger than when with the owner. Therefore, females were overall more social but seemed more affected than males by the owner’s absence. Parallels can be traced between our results and sex differences found in adult human romantic attachment, suggesting that the dog-owner bond has characteristics that are not found in the infant-mother relationship.
Inflation Risk?
Authors: De Grauwe Paul 
Published: August 2021

AbstractInflation is on the rise again in the industrialised world. This has led to fears of a sustained surge in inflation. This article argues that while such fears may make sense in the US, they do not in the eurozone, where the monetary-fiscal policy mix has been much less expansionary than in the US. The fear expressed by some that the monetary overhang from the large injections of liquidity through quantitative easing might lead to inflation in the eurozone does not stand up to scrutiny either. The conclusion offers some observations on the monetary operating procedures in the ECB. It argues that in the future, when interest rates rise again, the ECB risks transferring all (and even more) of its profits to the banking system. This article proposes a way to avoid this unacceptable outcome.
Hunting pressure modulates the composition and size structure of terrestrial and arboreal vertebrates in Amazonian forests
Authors: Scabin Andressa B. , Peres Carlos A. 
Published: August 2021

AbstractOverhunting is a leading contemporary driver of tropical forest wildlife loss. The absence or extremely low densities of large-bodied vertebrates disrupts plant-animal mutualisms and consequently degrades key ecosystem services. Understanding patterns of defaunation is therefore crucial given that most tropical forests worldwide are now “half-empty”. Here we investigate changes in vertebrate community composition and size structure along a gradient of marked anthropogenic hunting pressure in the Médio Juruá region of western Brazilian Amazonia. Using a novel camera trapping grid design deployed both in the understorey and the forest canopy, we estimated the aggregate biomass of several functional groups of terrestrial and arboreal species at 28 sites along the hunting gradient. Generalized linear models (GLMs) identified hunting pressure as the most important driver of aggregate biomass for game, terrestrial, and arboreal species, as well as nocturnal rodents, frugivores, and granivores. Local hunting pressure affected vertebrate community structure as shown by both GLM and ordination analyses. The size structure of vertebrate fauna changed in heavily hunted areas due to population declines in large-bodied species and apparent compensatory increases in nocturnal rodents. Our study shows markedly altered vertebrate community structure even in remote but heavily settled areas of continuous primary forest. Depletion of frugivore and granivore populations, and concomitant density-compensation by seed predators, likely affect forest regeneration in persistently overhunted tropical forests. These findings contribute to a better understanding of how cascading effects induced by historical defaunation operate, informing wildlife management policy in tropical peri-urban, rural and wilderness areas.
Two methods for the maximization of homogeneous polynomials over the simplex
Authors: Ahmed Faizan , Still Georg 
Published: August 2021

AbstractThe paper deals with the numerical solution of the problem P to maximize a homogeneous polynomial over the unit simplex. We discuss the convergence properties of the so-called replicator dynamics for solving P. We further examine an ascent method, which also makes use of the replicator transformation. Numerical experiments with polynomials of different degrees illustrate the theoretical convergence results.
Imagery Rescripting of Autobiographical Memories Versus Intrusive Images in Individuals with Disordered Eating
Authors: Kadriu Fortesa , Claes Laurence , Witteman Cilia , Krans Julie 
Published: August 2021

Abstract
Background
This study compared the effect of imagery rescripting (ImRs) of early autobiographical memories to ImRs of intrusive images and a no task control condition on eating disorder (ED) related core beliefs and ED symptoms in individuals at risk of developing an eating disorder. We qualitatively explored the content of ImRs scripts.

Method
Participants (N = 66, 87.8% females) were allocated to one of three conditions: ImRs of autobiographical memories, ImRs of intrusive images, or a no task control condition. Participants in the ImRs conditions received a 9-min self-guided ImRs intervention in the lab and practiced ImRs daily for the next 6 days online. Participants in the no task control condition took a 9-min break and did not do any further tasks. All participants attended a follow-up testing 1 week after the lab session.

Results
Both ImRs manipulations resulted in greater reductions in negative core beliefs and ED symptoms compared to the no task control group. However, there were no differences among the two ImRs groups. The most widely used rescripting strategy was self-compassion, followed by unhealthy, ED-confirming strategies. No significant associations were found between the ImRs strategies and any of the outcome measures.

Conclusion
ImRs may be a promising intervention for individuals with disordered eating and both early memories as well as intrusive images may be useful targets.