bluuebunny/crossref_metadata_2025_split huggingface dataset:
A SlMYB75-centred transcriptional cascade regulates trichome formation and sesquiterpene accumulation in tomato
Authors: Gong Zehao , Luo Yingqing , Zhang Wenfa , Jian Wei , Zhang Lu , Gao Xueli , Hu Xiaowei , Yuan Yujin , Wu Mengbo , Xu Xin , Zheng Xianzhe , Wu Guanle , Li Zhengguo , Li Zhi , Deng Wei 
Published: February 2021

Abstract
Tomato trichomes act as a mechanical and chemical barrier against pests. An R2R3 MYB transcription factor gene, SlMYB75, is highly expressed in type II, V, and VI trichomes. SlMYB75 protein is located in the nucleus and possesses transcriptional activation activity. Down-regulation of SlMYB75 increased the formation of type II, V, and VI trichomes, accumulation of δ-elemene, β-caryophyllene, and α-humulene in glandular trichomes, and tolerance to spider mites in tomato. In contrast, overexpression of SlMYB75 inhibited trichome formation and sesquiterpene accumulation, and increased plant sensitivity to spider mites. RNA-Seq analyses of the SlMYB75 RNAi line indicated massive perturbation of the transcriptome, with a significant impact on several classes of transcription factors. Expression of the MYB genes SlMYB52 and SlTHM1 was strongly reduced in the RNAi line and increased in the SlMYB75-overexpressing line. SlMYB75 protein interacted with SlMYB52 and SlTHM1 and activated their expression. SlMYB75 directly targeted the promoter of the cyclin gene SlCycB2, increasing its activity. The auxin response factor SlARF4 directly targeted the promoter of SlMYB75 and inhibited its expression. SlMYB75 also bound to the promoters of the terpene synthase genes SlTPS12, SlTPS31, and SlTPS35, inhibiting their transcription. Our findings indicate that SlMYB75 perturbation affects several transcriptional circuits, resulting in altered trichome density and metabolic content.
Comparison of Royal Canadian Navy Watchstanding Schedules
Authors: Paul Michel A , Love Ryan J 
Published: February 2021

ABSTRACT

Introduction
Life on board a naval vessel is exceptionally demanding. Workdays for naval sailors can quite easily become 18+ hours long when watch schedules, training, and drills/evolutions are taken into account. Rotating watches and short off-watch periods can force sailors into a biphasic sleep pattern that is not sufficiently restful or a rotating pattern that is impossible to adapt to.

Materials and Methods
Six different watch systems were evaluated over four separate at-sea trials. Engineering and tactical/combat departments have had different watch systems in the past because of constraints related to the specific environment in which they work. Therefore, two of the watch systems were engineering-specific watch evaluations, three of the systems were specific to tactical/combat departments, and one watch system was evaluated with the entire company of the naval vessel.

Results
Both two-section (1-in-2) watch systems and three-section (1-in-3) watch systems were evaluated, which involve two or three shifts of sailors rotating through a full continuous 24-h day, respectively. Moving beyond three rotations of sailors is impossible on Canadian naval vessels due to bunk space and other limitations. The best watch system that we evaluated with respect to fatigue and quality of life at sea was the 1-in-3 straight 8-h shift system that was tested for the entire ships’ company. The system has a single 8-h daily watch obligation (red watch, 4:00 am-12:00 pm; white watch 12:00 pm-8:00 pm; and blue watch, 8:00 pm-4:00 am). The best 1-in-2 system was the 8-4-4-8 system in which sailors are on-watch for 8 h, off-watch for 4 h, on-watch for 4 h, and then rest for 8 h. Both of these two systems have the advantage of equitably sharing the Window of Circadian Low (from about midnight to about 8:00 am), especially when melatonin concentration in the body is usually at its peak, between 2:00 am and 6:00 am.

Conclusions
The goal of this work was to comprehensively evaluate both submarine and surface fleet watch systems. We were able to develop alternative watch systems that increased Royal Canadian Navy operational readiness and improved the quality of life of our sailors at sea.
Leveraging the Existing Anesthesia Information Management System to Improve Anesthesia Quality Assurance Outcome Reporting
Authors: Kristobak Benjamin M , Jabaut Joshua M , Dickson Cody F , Cronin William A 
Published: February 2021

ABSTRACT

Introduction
Tracking measures of quality over time has been shown to improve care within institutions and across health systems. Perioperative quality assurance (QA) tracking by anesthesia departments in the Military Health System (MHS) has not used a uniform system integrated into the workflow of anesthesia providers. The purpose of this study was to demonstrate that the use of the embedded QA outcome reporting feature in the anesthesia information management system (AIMS) increased the rate of reporting compared to the current paper reporting system in a military anesthesia department.

Materials and Methods
An electronic outcome reporting mechanism embedded in the AIMS was activated as an alternative to paper QA outcome reporting. The proportion of anesthesia cases per month in a 12-month period with a reported QA outcome was compared to the previous year in which only the paper reporting system was used. The total number of cases in each time period with an outcome reported was compared using chi square for proportions, and systems were evaluated using the Statistical Process Control methodology. This project was evaluated and determined to be exempt from review by our institutional review board.

Results
There was a 389.8% increase in the number of cases with a QA outcome reported after the implementation of the outcome reporting function integrated into the AIMS (χ2 = 207.72; P &lt;.001, Table I). Systems before and after the intervention were stable, and special cause variation was noted only at the point of implementation of the electronic reporting system. Anesthesia providers were surveyed and felt that the addition of QA reporting to the AIMS made QA reporting more likely.

Conclusions
The use of an electronic QA outcome reporting method integrated into the AIMS dramatically increased the likelihood that a QA outcome would be reported. The decreased administrative burden of the integrated outcome reporting system was likely the primary reason for this increase. This study was limited by the fact that it was done in a single institution; however, the size and timing of the increase clearly indicate that the intervention was the reason for improved reporting. Electronic health record upgrades should consider incorporating QA reporting into the AIMS across the MHS. These measures could allow for system-wide improvement, evaluation, and evidence-based education on their own, but also by facilitating participation in the American Society of Anesthesiologists’ Anesthesia Quality Institute’s National Anesthesia Clinical Outcomes Registry. This report serves as a valuable example to institutions and perioperative leaders in the MHS of how to improve the robustness of perioperative QA reporting such that it could be used to validate and improve the value of care.
On the radio frequency dependence of the pulse delay phenomenon in PSR B0943+10
Authors: Suleymanova S A , Kazantsev A N , Rankin J M , Logvinenko S V 
Published: February 2021

ABSTRACT
We report the result of measurements of a gradual shift of the integrated pulses towards later spin phase of the anomalous pulsar B0943+10 at high radio frequencies. We have used observations from the Arecibo Observatory and the GMRT at 327 and 325 MHz correspondingly. For the measurements, we have proposed a special method for calculating the correct positions of the partially merged two components of the pulse profile shape with significant temporal changes in their amplitude ratio. The exponential change in the pulse phase with an amplitude of 4 ms and characteristic time of about 1 h has been found. Comparison of our measurements at 325 and 327 MHz with those at the lower frequencies of 25–80, 62 and 112 MHz have shown that the character of the process does not depend on frequency across a wide frequency range. The result is very important for constraining the nature of the delay. It supports the assumption that the process results from changes in the vacuum gap near the surface of the pulsar. The further correlation between changes in the pulse phase and its intensity is discussed.
Time delay lens modelling challenge
Authors: Ding X , Treu T , Birrer S , Chen G C-F , Coles J , Denzel P , Frigo M , Galan A , Marshall P J , Millon M , More A , Shajib A J , Sluse D , Tak H , Xu D , Auger M W , Bonvin V , Chand H , Courbin F , Despali G , Fassnacht C D , Gilman D , Hilbert S , Kumar S R , Lin J Y-Y , Park J W , Saha P , Vegetti S , Van de Vyvere L , Williams L L R 
Published: February 2021

ABSTRACT
In recent years, breakthroughs in methods and data have enabled gravitational time delays to emerge as a very powerful tool to measure the Hubble constant H0. However, published state-of-the-art analyses require of order 1 yr of expert investigator time and up to a million hours of computing time per system. Furthermore, as precision improves, it is crucial to identify and mitigate systematic uncertainties. With this time delay lens modelling challenge, we aim to assess the level of precision and accuracy of the modelling techniques that are currently fast enough to handle of order 50 lenses, via the blind analysis of simulated data sets. The results in Rungs 1 and 2 show that methods that use only the point source positions tend to have lower precision ($10\!-\!20{{\ \rm per\ cent}}$) while remaining accurate. In Rung 2, the methods that exploit the full information of the imaging and kinematic data sets can recover H0 within the target accuracy (|A| &lt; 2 per cent) and precision (&lt;6 per cent per system), even in the presence of a poorly known point spread function and complex source morphology. A post-unblinding analysis of Rung 3 showed the numerical precision of the ray-traced cosmological simulations to be insufficient to test lens modelling methodology at the percent level, making the results difficult to interpret. A new challenge with improved simulations is needed to make further progress in the investigation of systematic uncertainties. For completeness, we present the Rung 3 results in an appendix and use them to discuss various approaches to mitigating against similar subtle data generation effects in future blind challenges.
QSO photometric redshifts using machine learning and neural networks
Authors: Curran S J , Moss J P , Perrott Y C 
Published: February 2021

ABSTRACT
The scientific value of the next generation of large continuum surveys would be greatly increased if the redshifts of the newly detected sources could be rapidly and reliably estimated. Given the observational expense of obtaining spectroscopic redshifts for the large number of new detections expected, there has been substantial recent work on using machine learning techniques to obtain photometric redshifts. Here, we compare the accuracy of the predicted photometric redshifts obtained from deep learning (DL) with the k-nearest neighbour (kNN) and the decision tree regression (DTR) algorithms. We find using a combination of near-infrared, visible, and ultraviolet magnitudes, trained upon a sample of Sloan Digital Sky Survey quasi-stellar objects, that the kNN and DL algorithms produce the best self-validation result with a standard deviation of σΔz = 0.24 (σΔz(norm) = 0.11). Testing on various subsamples, we find that the DL algorithm generally has lower values of σΔz, in addition to exhibiting a better performance in other measures. Our DL method, which uses an easy to implement off-the-shelf algorithm with neither filtering nor removal of outliers, performs similarly to other, more complex, algorithms, resulting in an accuracy of Δz &lt; 0.1 up to z ∼ 2.5. Applying the DL algorithm trained on our 70 000 strong sample to other independent (radio-selected) data sets, we find σΔz ≤ 0.36 (σΔz(norm) ≤ 0.17) over a wide range of radio flux densities. This indicates much potential in using this method to determine photometric redshifts of quasars detected with the Square Kilometre Array.
Constraining velocity-dependent self-interacting dark matter with the Milky Way’s dwarf spheroidal galaxies
Authors: Correa Camila A 
Published: February 2021

ABSTRACT
The observed anticorrelation between the central dark matter (DM) densities of the bright Milky Way (MW) dwarf spheroidal galaxies (dSphs) and their orbital pericentre distances poses a potential signature of self-interacting dark matter (SIDM). In this work, we investigate this possibility by analysing the range of SIDM scattering cross-section per unit mass, σ/mχ, able to explain such anticorrelation. We simulate the orbital evolution of dSphs subhaloes around the MW assuming an analytical form for the gravitational potential, adopting the proper motions from the Gaia mission and including a consistent characterization of gravitational tidal stripping. The evolution of subhalo density profiles is modelled using the gravothermal fluid formalism, where DM particle collisions induce thermal conduction that depends on σ/mχ. We find that models of dSphs, such as Carina and Fornax, reproduce the observed central DM densities with fixed σ/mχ ranging between 30 and 50 cm2 g−1, whereas other dSphs prefer larger values ranging between 70 and 100 cm2 g−1. These cross-sections correlate with the average collision velocity of DM particles within each subhalo’s core, so that systems modelled with large cross-sections have lower collision velocities. We fit the cross-section–velocity correlation with a SIDM particle model, where a DM particle of mass mχ = 53.93 ± 9.81 GeV interacts under the exchange of a light mediator of mass mϕ = 6.6 ± 0.43 MeV, with the self-interactions being described by a Yukawa potential. The outcome is a cross-section–velocity relation that explains the diverse DM profiles of MW dSph satellites and is consistent with observational constraints on larger scales.
Conditions in the WR 140 wind-collision region revealed by the 1.083-μ m He i line profile
Authors: Williams Peredur M , Varricatt Watson P , Chené André-Nicolas , Corcoran Michael F , Gull Ted R , Hamaguchi Kenji , Moffat Anthony F J , Pollock Andrew M T , Richardson Noel D , Russell Christopher M P , Sander Andreas A C , Stevens Ian R , Weigelt Gerd 
Published: February 2021

ABSTRACT
We present spectroscopy of the P Cygni profile of the 1.083-$\mu$m He i line in the WC7+O5 colliding-wind binary (CWB) WR 140 (HD 193793), observed in 2008, before its periastron passage in 2009, and in 2016–2017, spanning the subsequent periastron passage. Both absorption and emission components showed strong variations. The variation of the absorption component as the O5 star was occulted by the wind-collision region (WCR) sets a tight constraint on its geometry. While the sightline to the O5 star traversed the WCR, the strength and breadth of the absorption component varied significantly on time-scales of days. An emission subpeak was observed on all our profiles. The variation of its radial velocity with orbital phase was shown to be consistent with formation in the WCR as it swung round the stars in their orbit. Modelling the profile gives a measure of the extent of the subpeak-forming region. In the phase range 0.93–0.99, the flux in the subpeak increased steadily, approximately inversely proportionally to the stellar separation, indicating that the shocked gas in the WCR where the line was formed was adiabatic. After periastron, the subpeak flux was anomalously strong and varied rapidly, suggesting formation in clumps downstream in the WCR. For most of the time, its flux exceeded the 2–10-keV X-ray emission, showing it to be a significant coolant of the shocked wind.
Iron–sulfur biology invades tRNA modification: the case of U34 sulfuration
Authors: Zhou Jingjing , Lénon Marine , Ravanat Jean-Luc , Touati Nadia , Velours Christophe , Podskoczyj Karolina , Leszczynska Grazyna , Fontecave Marc , Barras Frédéric , Golinelli-Pimpaneau Béatrice 
Published: February 2021

Abstract
Sulfuration of uridine 34 in the anticodon of tRNAs is conserved in the three domains of life, guaranteeing fidelity of protein translation. In eubacteria, it is catalyzed by MnmA-type enzymes, which were previously concluded not to depend on an iron–sulfur [Fe–S] cluster. However, we report here spectroscopic and iron/sulfur analysis, as well as in vitro catalytic assays and site-directed mutagenesis studies unambiguously showing that MnmA from Escherichia coli can bind a [4Fe–4S] cluster, which is essential for sulfuration of U34-tRNA. We propose that the cluster serves to bind and activate hydrosulfide for nucleophilic attack on the adenylated nucleoside. Intriguingly, we found that E. coli cells retain s2U34 biosynthesis in the ΔiscUA ΔsufABCDSE strain, lacking functional ISC and SUF [Fe–S] cluster assembly machineries, thus suggesting an original and yet undescribed way of maturation of MnmA. Moreover, we report genetic analysis showing the importance of MnmA for sustaining oxidative stress.
Sugar-sweetened beverage consumption, weight gain, and risk of type 2 diabetes and cardiovascular diseases in Asia: a systematic review
Authors: Neelakantan Nithya , Park Su Hyun , Chen Guo-Chong , van Dam Rob M 
Published: February 2021

Abstract

Context
The prevalence of type 2 diabetes mellitus (T2DM) and cardiovascular diseases (CVDs) is increasing in Asia and several countries are adopting preventive policies to reduce consumption of sugar-sweetened beverages (SSBs). However, evidence on the relation between SSB consumption and cardiometabolic health in Asian populations has not been summarized.

Objective
In this systematic review, the associations between consumption of SSBs and cardiometabolic outcomes, including obesity, T2DM, and CVD, are examined in Asian populations.

Data sources
The PubMed, Scopus, and Web of Science databases, and gray literature were searched up to October, 2020 to identify relevant articles.

Data extraction
Two investigators independently extracted data from included studies.

Data analysis
When sufficient studies were available, a random-effects meta-analysis was used to calculate the pooled estimates (expressed as risk ratio [RR] and 95% confidence interval [CI]). Heterogeneity was tested and quantified using the Cochrane Q test and I2 statistic, respectively.

Results
Of the 17 studies included, 8 provided results about measures of adiposity (3 on weight change, 4 on body mass index (BMI), 2 on percent body fat, and 1 on abdominal obesity), 6 reported results about T2DM, and 3 reported on different CVD outcomes. High SSB consumption was significantly associated with greater weight gain and with a higher risk of selected CVD outcomes as compared with low consumption. In the meta-analysis, high SSB consumption was associated with greater T2DM risk before (RR, 1.51; 95%CI, 1.15–1.98 for highest vs lowest category; I2 = 76%) and after (RR, 1.38; 95%CI, 1.09–1.73; I2 = 56%) adjustment for BMI.

Conclusions
These findings suggest that high SSB consumption is directly associated with weight gain, risk of T2DM, and, possibly, selected CVD outcomes in Asian populations. Public health strategies to reduce SSB consumption in Asian countries are warranted.

Systematic Review Registration
PROSPERO registration no. CRD42019129456.Keywords: adiposity. cardiovascular disease, Asia, sugar-sweetened beverages, type 2 diabetes.
The berry health tool chest – an evidence map and interactive resource
Authors: Kay Kristine L , Strauch Renee C , Granillo Cheryl D , Bame Megan W , Xiong Jia , Mast Aubrey C , Burton-Freeman Britt , Kay Colin D , Lila Mary Ann 
Published: February 2021

AbstractBerry consumption is linked to diverse health benefits, but numerous questions remain regarding mechanism of action, dose efficacy, and optimal duration and frequency of intake. Addressing these outstanding questions requires an organized assessment of current research, to inform future study designs and fill critical knowledge gaps. Tools that organize such information will also facilitate consumer messaging, targeted nutritional health initiatives, and dietary intake guidelines. This review aimed to describe the development and utility of the “Berry Health Tool Chest,” an evidence map summarizing trial design features of studies characterizing the impact of berry consumption upon human health biomarkers. A systematic search strategy identified relevant high-quality human feeding studies, whose study design parameters were collected and compiled into an evidence map that is freely available as an interactive online interface enabling tabulated data to be interrogated, filtered, and exported. Of the 231 included studies, approximately 70% were of less than 3 months’ duration and/or fewer than 50 participants, illustrating research gaps that could potentially inform the design of future studies.
Mass Media and the Colonial Informant: Messaoud Djebari and the French Empire, 1880–1901
Authors: Asseraf Arthur 
Published: February 2021

Abstract
The nineteenth century saw an explosion of mass media as well as an expansion of colonial states. These two processes mutually influenced each other, and at the intersection lay a thin layer of individuals who could gain inordinate power to influence global information flows. This article follows the career of one such individual, Messaoud Djebari, an Algerian man who generated several controversies by fabricating information first in Tunisia in 1881, and then across West Africa and France in 1892–5. Djebari’s case suggests that some men trained to act as colonial intermediaries could end up playing important roles in shaping the circulation of information well beyond their territory of origin. Colonial informants were defined less by their role within a given colonial territory than by an ability to portray themselves as conduits to valuable information inaccessible to Europeans, irrespective of the location. Beyond the colonial context, this calls our attention to a particular practice of information manipulation characteristic of this age: the art of making oneself into a conduit by passing off one’s opinions as somebody else’s information.
Bacterial diketopiperazines stimulate diatom growth and lipid accumulation
Authors: Sittmann John , Bae Munhyung , Mevers Emily , Li Muzi , Quinn Andrew , Sriram Ganesh , Clardy Jon , Liu Zhongchi 
Published: February 2021

Abstract
Diatoms are photosynthetic microalgae that fix a significant fraction of the world’s carbon. Because of their photosynthetic efficiency and high-lipid content, diatoms are priority candidates for biofuel production. Here, we report that sporulating Bacillus thuringiensis and other members of the Bacillus cereus group, when in co-culture with the marine diatom Phaeodactylum tricornutum, significantly increase diatom cell count. Bioassay-guided purification of the mother cell lysate of B. thuringiensis led to the identification of two diketopiperazines (DKPs) that stimulate both P. tricornutum growth and increase its lipid content. These findings may be exploited to enhance P. tricornutum growth and microalgae-based biofuel production. As increasing numbers of DKPs are isolated from marine microbes, the work gives potential clues to bacterial-produced growth factors for marine microalgae.