bluuebunny/crossref_metadata_2025_split huggingface dataset:
Assessment of ecotoxical state of technologically modified edaphotopes with waste of oil refinery with the Allium-test method
Authors:   Drohobych Ivan Franko State Pedagogical University, Klepach H. , Holub N. ,   Ivan Franko National University of Lviv, Lupak O. ,   Drohobych Ivan Franko State Pedagogical University
Published: July 2021

The ecotoxic state of technologically modified edaphotopes by waste of refinery was investigated with the Allium-test method. Material for the investigations was samples of edaphotopes which were selected at a depth of 15–20 cm from seven research areas of different locations. The last ones were 10–200 m away in relation to the surface storages of oil sludge and other waste of refinery. It has been detected in Allium-test that edaphotopes of the research areas located at a distance of 10–50 m relative to the storages of waste are toxic. Particularly, their aqueous extracts cause a medium and high level of phyto-, cyto- and genotoxic effects on the growing and proliferative activity of Allium cepa Stuttgarter Riesen variety meristem. Test extracts of edaphotopes in growing Allium-test caused darkening of rootlets and blackening of their tips (contrary to the light rootlets with light tips in control). In addition to it the growing activity of A. сepa bulb roots that were grown on the aqueous extracts of edaphotopes was decreased in 1.71–3.5 times depending on the location and measure of remoteness of the research area from the storage of waste of the refinery. Phytotoxicity of aqueous extracts of those edaphotopes regarding to A. сepa is above average and higher levels, and tolerance of onion rootlets to the test extracts decreases up to 29–52 % comparing to control. The mitotic activity of apical meristem of rootlets also decreased. Particularly, mitotic indexes diminished to 51.05–63.57 % (in control – 82.34 %) and cytotoxicity ones were in the range of 22.8–48.0 % that confirms the medial level of cytotoxicity of the investigated edaphotopes. Ana-telophase method and micronucleus test have shown that the chromosome aberrations frequency and appearance of micronuclei in the cells of the apical meristem of the test object A. cepa is higher in 3.34–5.20 times comparing to control. It indicates the average and the high level of genotoxicity of the investigated edaphotopes. The edaphotopes, which are located at the distance of 100–200 m in relation to the surface storages of waste of refinery have a slight phyto-, cyto- and genotoxic effects which are close to the background level.
Excess Screen Time - Impact on Childhood Development and Management: A Review
Authors: Karki Utkarsh , Sravanti Lakshmi 
Published: July 2021

In recent years children are immersed in both traditional and new forms of digital media around the globe. Screen time is now embedded in the daily lives of young children beginning from infancy. Pre-schoolers exposed to screen and interactive media may benefit in many ways but excessive and inappropriate screen exposure could potentially lead to several health and developmental risks. Environmental factors such as parents, siblings, peers have a profound influence on a child’s development. Despite challenges of screen media parenting practices and those posed by lockdown (work from home, online schooling) during this pandemic, parents hold the key to navigate these challenges by serving as role models to ensure positive developmental outcomes in their children.
Experience of organization of the course " the Basics programming for schoolchildren " by the center of pre-university training in a pandemic
Authors: Litvak E. G. , Bradul N. V. 
Published: July 2021

The article discusses the processes of designing, developing and launching an online course "The basics of programming for schoolchildren" for the center of pre-university training in a pandemic. The choice of the top-level design model SAM1 for courses that need to be developed and launched in a short time without complete data on the target audience is substantiated. The advantages of choosing a backward design model for developing the content of the target course are shown. Using the method of backward design, the course "The basics of programming for schoolchildren" was designed and developed. The program of the course is presented in the form of a sequence of modules and individual topics. The use of gamification elements based on the "VKontakte" social network has been substantiated. Examples of educational activities are given. A link is given to the developed course posted in LMS Moodle Donetsk Academy of Management and Public Administration.
A STUDY OF HYPERURICACIDEMIA AND THE PRESENCE AND SEVERITY OF ACUTE CORONARY SYNDROME IN INDEX MEDICAL COLLEGE, HOSPITAL AND RESEARCH CENTER, INDORE ( M.P.)
Authors: Khanam B. , Khan M. Imran , Singh Ajay Kumar , Solanki Sumit , Holkar S.M. 
Published: July 2021

Background: Few studies have assessed the relation of hyperuricacidemia with the acute coronary syndrome (ACS). This study investigated the association between high uric acid levels with the presence and severity of ACS.
Methods: Three hundred and seventy patients having angiographic evidence of atherosclerosis (CAD + case group) compared to 170 patients with no luminal stenosis (n=110) or with <50% luminal stenosis (n=60) at coronary angiography (CAD – control group).
Results: The mean age of the patients was 60 ± 10 years (317 men, 58.7%). Hyperuricacidemia was more likely associated with a trend toward higher vessel scores, indicating a more severe CAD (adjusted OR=1.51, 95% CI=1.09-2.09; P=0.005) in the whole population. A comparison of sex-specific values showed a significant association existed only in men.
Conclusions: Asymptomatic hyperuricacidemia may be associated with the presence and severity of ACS.
Keywords: Hyperuricacidemia, Severity & Acute Coronary Syndrome.
QUALIDADE DE OVOS DE PRODUÇÃO ORGÂNICA AO LONGO DE CINQUENTA SEMANAS DE POSTURA
Authors: Poletti Bruna , Vieira Maitê de Moraes , Pinto Andrea Troller , Ferreira Jeruza Indiara , Do Nascimento Vladimir Pinheiro 
Published: July 2021

Atualmente, a produção de ovos em sistemas alternativos vem crescendo, porém não há informações relativas aos seus critérios de qualidade. Com o objetivo de analisar características físico-químicas de ovos orgânicos, associadas ao avanço da idade de postura das aves, avaliou-se peso do ovo, altura de albúmen, espessura da casca, índice e porcentagem de gema, porcentagem de albúmen e de casca e unidade Haugh (UH). Quinzenalmente, seis ovos frescos foram avaliados, durante a 28ª e 80ª semana de postura. A qualidade externa e interna dos ovos foi afetada pela idade. Peso do ovo e espessura da casca resultaram em efeito cúbico e quadrático (P<0,001), respectivamente. Avaliação da UH e da porcentagem de gema e albúmen resultaram em comportamento cúbico (P<0,05). Índice de gema teve comportamento linear decrescente. O aumento da idade das aves ocasionou diminuição de indicadores de qualidade interna. Apesar da redução na qualidade, ovos de sistema orgânico mantiveram os padrões adequados para consumo, ao longo do ciclo produtivo. Com o avanço da idade, os ovos ficaram mais pesados, porém com redução da espessura de casca, da unidade Haugh e do índice e porcentagem de gema.
Budget Impact analysis of ponatinib for the management of patients with chronic myeloid leukemia
Authors: Basile Michele , Rumi Filippo , Cicchetti Americo 
Published: July 2021

Background: The current clinical practice for patients affected by chronic myeloid leukemia (CML) is based on the evaluation of second generation alternatives following therapeutic failure that leads to a lengthening of patients’ management times and a consequent negative impact in terms of quality of life.
Objective: To determine the economic absorption of resources associated to the management of patients with CML in a scenario in which an early recourse to ponatinib is considered as compared with a scenario based on the current Italian clinical practice characterized by a cyclical recourse to the available therapies.
Methods: A Budget Impact model was developed to compare the resources absorbed in the scenarios under assessment considering a 3-year time horizon and the perspective of the Italian National Health Service. Results are expressed in terms of differential resources absorbed in the alternative scenarios.
Results: The increase in the recourse to ponatinib allowed a saving of resources for the Italian NHS over the 3-year time horizon of –€ 1,979,322 (€ 825,104,350 vs € 823,125,028). The parameter affecting the most of the results achieved in the base-case is the monthly cost of bosutinib used as a third-line treatment.
Conclusions: The increase in the recourse to ponatinib in patients affected by CML that failed to respond to a previous pharmacological therapy resulted to be associated to a lower level of resources’ absorption in the Italian NHS allowing to re-allocate health founds to other fields of the care sector ensuring greater sustainability of the system.
An Analysis of Student Difficulties in Mathematics Learning in terms of Student Mathematical Connection Ability on Pythagoras Theorem
Authors: Sholeha Viona Aida , Risnawati Risnawati , Habibullah Habibullah 
Published: July 2021

This research aimed to describe student difficulties in mathematics learning in terms of student mathematical connection ability on Pythagoras theorem. This research was a qualitative descriptive research with case study design. The research subjects were 18 the IX grade students, then reduced to 5 students and purposive sampling technique was used in this research. Triangulation data such as mathematical connection ability and difficulties of mathematic learning tests and interview were used for collecting the data. The data were analyzed by Miles and Hubermen techniques including three stages: reduction, presentation, and conclusion/verification. The findings of this research showed that, each respondent has different difficulties at each mathematical connection ability level; (1) The subject (very high) mathematical connection ability level did not have problem with all indicators of difficulties in mathematics learning; (2) The subject (high) mathematical connection ability level had associations or visual-motor combination; (3) The subject (medium) mathematical connection ability level had associations or visual-motor combination and difficulties in recognizing and using symbols; (4) The subject (low) mathematical connection ability level had little spatial disruption, association or visual-motor combination, and little difficulties in recognizing and using symbols; (5) The subject (very low) mathematical connection ability level had spatial disruption, association or visual-motor combination, and difficulties in recognizing and using symbols
The neural correlates of consciousness under the free energy principle: From computational correlates to computational explanation
Authors: Wiese Wanja , Friston Karl J. 
Published: July 2021

How can the free energy principle contribute to research on neural correlates of consciousness, and to the scientific study of consciousness more generally? Under the free energy principle, neural correlates should be defined in terms of neural dynamics, not neural states, and should be complemented by research on computational correlates of consciousness – defined in terms of probabilities encoded by neural states.
We argue that these restrictions brighten the prospects of a computational explanation of consciousness, by addressing two central problems. The first is to account for consciousness in the absence of sensory stimulation and behaviour. The second is to allow for the possibility of systems that implement computations associated with consciousness, without being conscious, which requires differentiating between computational systems that merely simulate conscious beings and computational systems that are conscious in and of themselves.
Given the notion of computation entailed by the free energy principle, we derive constraints on the ascription of consciousness in controversial cases (e.g., in the absence of sensory stimulation and behaviour). We show that this also has implications for what it means to be, as opposed to merely simulate a conscious system.
Effect of ISM1 on the Immune Microenvironment and Epithelial-Mesenchymal Transition in Colorectal Cancer
Authors: Wu Yuhui , Liang Xiaojing , Ni Junjie , Zhao Rongjie , Shao Shengpeng , Lu Si , Han Weidong , Yu Liangliang 
Published: July 2021

Background: An increasing number of studies have shown that Isthmin 1 (ISM1), a secreted protein, is important in tumorigenesis and invasion, including in colorectal cancer (CRC). However, the mechanisms are still unclear. This study aims to explore the function and prognosis capacity of ISM1 in CRC.Methods: We investigated the expression of ISM1 in 18 CRC tissues vs. adjacent normal tissues from GSE50760, 473 CRC tissues vs. 41 normal tissues from The Cancer Genome Atlas (TCGA), and across gastrointestinal cancer types. Differences were further confirmed in CRC tissues via quantitative real-time polymerase chain reaction (qRT-PCR). Then, we analyzed correlations between clinicopathologic features and ISM1 expression, including prognostic prediction value, using the Kaplan–Meier method and multivariate Cox regression. Gene set enrichment analysis (GSEA) was performed to identify ISM1-related pathways. In vitro experiments were performed to verify the role of ISM1 in epithelial-mesenchymal transition (EMT) and CRC progression.Results: Multiple datasets showed that ISM1 is upregulated in CRC tissues, which was validated. Patients with higher ISM1 expression had shorter overall survival (OS), and ISM1 expression served as an independent prognostic factor. Enrichment analysis showed that ISM1 upregulation was positively correlated with cancer-related pathways, such as EMT, hypoxia, and the Notch and KRAS signaling pathways. We were exclusively interested in the connection between ISM1 and EMT because 71% of genes in this pathway were significantly positively co-expressed with ISM1, which may account for why patients with higher ISM1 expression are prone to regional lymph node involvement and progression to advanced stages. In addition, we found that ISM1 was positively correlated with multiple immunosuppressive pathways such as IL2/STAT5, TNF-α/NF-κB, and TGF-β, and immune checkpoints, including PD-L1, PD-1, CTLA-4, and LAG3, which may account for upregulation of ISM1 in immunotherapy-resistant patients. Notably, through in vitro experiments, we found that ISM1 promoted EMT and colon cancer cell migration and proliferation.Conclusion: ISM1 is critical for CRC development and progression, which enhances our understanding of the low response rate of CRC to immunotherapy via immunosuppressive signaling pathways.
Biliary Neuroendocrine Neoplasms: Analysis of Prognostic Factors and Development and Validation of a Nomogram
Authors: Zhou Shengnan , Jiang Shitao , Chen Weijie , Yin Haixin , Dong Liangbo , Zhao Hao , Han Shaoqi , He Xiaodong 
Published: July 2021

BackgroundFor this study, we explored the prognostic profiles of biliary neuroendocrine neoplasms (NENs) patients and identified factors related to prognosis. Further, we developed and validated an effective nomogram to predict the overall survival (OS) of individual patients with biliary NENs.MethodsWe included a total of 446 biliary NENs patients from the SEER database. We used Kaplan-Meier curves to determine survival time. We employed univariate and multivariate Cox analyses to estimate hazard ratios to identify prognostic factors. We constructed a predictive nomogram based on the results of the multivariate analyses. In addition, we included 28 biliary NENs cases from our center as an external validation cohort.ResultsThe median survival time of biliary NENs from the SEER database was 31 months, and the value of gallbladder NENs (23 months) was significantly shorter than that of the bile duct (45 months) and ampulla of Vater (33.5 months, p=0.023). Multivariate Cox analyses indicated that age, tumor size, pathological classification, SEER stage, and surgery were independent variables associated with survival. The constructed prognostic nomogram demonstrated good calibration and discrimination C-index values of 0.783 and 0.795 in the training and validation dataset, respectively.ConclusionAge, tumor size, pathological classification, SEER stage, and surgery were predictors for the survival of biliary NENs. We developed a nomogram that could determine the 3-year and 5-year OS rates. Through validation of our central database, the novel nomogram is a useful tool for clinicians in estimating individual survival among biliary NENs patients.
Comparison of Oncological Outcomes Between Radical Prostatectomy and Radiotherapy by Type of Radiotherapy in Elderly Prostate Cancer Patients
Authors: Guo Xiao-Xiao , Xia Hao-Ran , Hou Hui-Min , Liu Ming , Wang Jian-Ye 
Published: July 2021

ObjectiveWe aimed compare the oncologic outcomes of radical prostatectomy (RP) with those of external beam radiotherapy (EBRT), brachytherapy (BT), or EBRT + BT (EBBT) in elderly patients with localised prostate cancer (PCa).MethodsLocalised PCa patients aged ≥70 years who underwent RP, EBRT, BT, or EBBT between 2004 and 2016 were identified from the Surveillance, Epidemiology, and End Results database. Multivariable competing risks survival analyses were used to estimate prostate cancer-specific mortality (CSM) and other-cause mortality (OCM). Subgroup analyses according to risk categories were also conducted.ResultsOverall, 14057, 37712, 8383, and 5244 patients aged ≥70 years and treated with RP, EBRT, BT, and EBBT, respectively, were identified. In low- to intermediate-risk patients, there was no significant difference in CSM risk between RP and the other three radiotherapy modalities (all P &gt; 0.05). The corresponding 10-year CSM rates for these patients were 1.2%, 2.3%, 2.0%, and 1.8%, respectively. In high-risk patients, EBRT was associated with a higher CSM than RP (P = 0.003), whereas there was no significant difference between RP and BT or RP and EBBT (all P &gt; 0.05). The 10-year CSM rates of high-risk patients in the RP, EBRT, BT, and EBBT groups were 7.5%, 10.2%, 8.3%, and 7.6%, respectively. Regarding OCM, the risk was generally lower in RP than in the other three radiotherapy modalities (all P &lt; 0.001).ConclusionsAmong men aged ≥70 years with localised PCa, EBRT, BT, and EBBT offer cancer-specific outcomes similar to those of RP for individuals with low- to intermediate-risk disease. In patients with high-risk disease, EBBT had outcomes equally favourable to those of RP, but RP is more beneficial than EBRT. More high-quality trials are warranted to confirm and expand the present findings.
The Albumin-to-Fibrinogen Ratio Independently Predicts Acute Kidney Injury in Infants With Ventricular Septal Defect Undergoing Cardiac Surgery With Cardiopulmonary Bypass
Authors: Cao Fan , Chen Xinxin , Huang Guodong , Liu Wenhua , Zhou Na , Yuan Huili , Zou Minghui 
Published: July 2021

Background: Acute kidney injury (AKI) is a common and serious complication faced by children following ventricular septal defect (VSD) surgery with cardiopulmonary bypass (CPB). The objective of this study was to explore potential predictors inherent to AKI.Methods: VSD infants who were scheduled for elective cardiac surgery with CPB from 2017 to 2020 were enrolled in this study. Based on the Pediatric Risk, Injury, Failure, Loss, End-Stage Renal Disease (pRIFLE) criteria, patients were divided into AKI and non-AKI groups. Univariate and multivariate logistic regression analyses were carried out in order to evaluate potential risk factors for AKI. Receiver operating characteristic (ROC) curves were generated to evaluate the predictive probabilities of risk factors for AKI.Results: Of all the 338 enrolled VSD infants, 49 manifested AKI with an incidence of 14.5% (49/338). The ROC curve indicated that albumin-to-fibrinogen ratio (AFR) during CPB was a significant predictor of AKI [area under the curve (AUC), 0.711; p &lt; 0.001]. Based on the univariate and multivariate logistic analyses, AFR during CPB [odds ratio (OR), 1.89; 95% confidence interval (CI), 1.22–2.76, p = 0.011] was the only independent risk factor for AKI.Conclusions: This study demonstrated that a low AFR (&lt;9.35) during CPB was an independent risk factor for AKI in VSD infants following cardiac surgery with CPB.
Biological Control of Pear Valsa Canker Caused by Valsa pyri Using Penicillium citrinum
Authors: Yuan Hongbo , Shi Bingke , Huang Tianxiang , Zhou Zengqiang , Wang Li , Hou Hui , Tu Hongtao 
Published: July 2021

Valsa canker caused by Valsa pyri is one of the most destructive diseases of commercial pear. For the present analysis, 29 different endophytic fungal strains were isolated from the branches of a healthy pear tree. In dual culture assays, strain ZZ1 exhibited robust antifungal activity against all tested pathogens including Valsa pyri. Microscopic analyses suggested that following co-culture with ZZ1, the hyphae of V. pyri were ragged, thin, and ruptured. ZZ1 also induced significant decreases in lesion length and disease incidence on detached pear branches inoculated with V. pyri. ZZ1 isolate-derived culture filtrates also exhibited antifungal activity against V. pyri, decreasing mycelial growth and conidium germination and inhibiting V. pyri-associated lesion development on pear branches. These results suggest that the ZZ1 isolate has the potential for use as a biological control agent against V. pyri. The strain was further identified as Penicillium citrinum based on its morphological characteristics and molecular analyses. Overall, these data highlight a potentially valuable new biocontrol resource for combating pear Valsa canker.
Low C/N Ratios Promote Dissimilatory Nitrite Reduction to Ammonium in Pseudomonas putida Y-9 under Aerobic Conditions
Authors: Huang Xuejiao , Tie Wenzhou , Xie Deti , Li Zhenlun 
Published: July 2021

The biogeochemical consequences of denitrification and dissimilatory nitrate reduction to ammonium (DNRA) have a significant influence on nitrogen (N) cycling in the ecosystem. Many researchers have explored these two pathways in soil and sediment ecosystems under anaerobic conditions. However, limited information is available regarding the influence of external environmental conditions on these two pathways in a well-defined experimental system under aerobic conditions. In this study, the impacts of the external environmental factors (carbon source, C/N ratio, pH, and dissolved oxygen) on nitrite reduction through the denitrification and DNRA routes in Pseudomonas putida Y-9 were studied. Results found that sodium citrate and sodium acetate favored denitrification and DNRA, respectively. Furthermore, neutral pH and aerobic conditions both facilitated DNRA and denitrification. Especially, low C/N ratios motivated the DNRA while high C/N ratios stimulated the denitrification, which was opposite to the observed phenomena under anaerobic conditions.