bluuebunny/crossref_metadata_2025_split huggingface dataset:
Metformin Sensitizes Osteosarcoma Cells to Chemotherapy Through IGF-1R/miR-610/FEN1 Pathway
Authors: Dong Suwei , Xiao Yanbin , Zhu Ziqiang , Ma Xiang , Peng Zhuohui , Kang Jianping , Xie Biao , Wang Jianqiang , Li Zhen , Wang Yunqing 
Published: January 2021

Abstract
Background: Due to constitutive or acquired non-sensitive to cytotoxic agents, the prognosis of osteosarcoma remains unfavorable. It’s has been proved that metformin could enhance the chemosensitivity of cancer cells to anticancer drugs. A novel finding states that IGF-1R involves in cancer chemoresistance, However, whether IGF-1R play a role in metformin-induced osteosarcoma chemosensitivity is incompletely understood. Hence, the current study aimed to elucidate the role of metformin in OS cell chemosensitivity modulation to identify the underlying mechanism of metformin regulating the IGF-1R/miR-610/FEN1 signaling.Methods: Immunohistochemistry and qRT-PCR were used to evaluate the expression pattern of IGF-1R, miR-610 and FEN1 in osteosarcoma and paired normal tissues. Western blot and qRT-PCR were performed to determine changes in expression of key molecules in the IGF-1R/miR-610/FEN1 signaling pathway after various treatments. The direct modulation between miR-610 and FEN1 was monitored by luciferase reporter assay. Osteosarcoma cell sensitivity to chemotherapy was detected by MTS assay. In vivo experiments were conducted to further verify the role of the metformin in the chemosensitivity modulation of OS cells to ADM.Results: We found that IGF-1R, miR-610 and FEN1 were abberently expressed in osteosarcoma, and participated in apoptosis modulation (p < 0.05). We found that this effect was abated by metformin treatment. Luciferase reporter assays confirmed that FEN1 is a direct target of miR-610. Moreover, we observed that metformin treatment decreased IGF-1R and FEN1, but elevated miR-610 expression. Metformin sensitized OS cells to cytotoxic agents, while overexpression of FEN1 compromised the sensitizing effects of metformin partly. Furthermore, metformin was observed to enforce the ADM treatment effect in nude mice xenograft models.Conclusions: Overall, metformin enhanced the sensitivity of OS cells to cytotoxic agents via the IGF-1R/miR-610/FEN1 signaling axis, highlighting the capacity of metformin as an adjunct to the chemotherapy of OS.
Preoperative Beta-Blocker in Ventricular Dysfunction Patients: Need a More Granular Quality Metric.
Authors: Tang Hanwei , Chen Kai , Hou Jianfeng , Huang Xiaohong , Liu Sheng , Hu Shengshou 
Published: January 2021

Abstract
BackgroundThe use of preoperative beta-blockers has been accepted as a quality standard for patients undergoing coronary artery bypass graft (CABG) surgery. However, conflicting results from recent studies have raised questions concerning the effectiveness of this quality metric. We sought to determine the influence of preoperative beta-blocker administration before CABG in patients with left ventricular dysfunction.MethodsThe authors analyzed all cases of isolated CABGs in patients with left ventricular ejection fraction less than 50%, performed between 2012 January and 2017 June, at 94 centres recorded in the China Heart Failure Surgery Registry database. In addition to the use of multivariate regression models, a 1 to 1 propensity scores matched analysis was performed.ResultsOf 6,116 eligible patients, 61.7% received a preoperative beta-blocker. No difference in operative mortality was found between two cohorts (3.7% for the non-beta-blockers group vs 3.0% for the beta-blocker group; adjusted odds ratio [OR], 0.82 [95% CI, 0.58-1.15]). Few differences in the incidence of other postoperative clinical end points were observed as a function of preoperative beta-blockers except in stroke (0.7% for the non-beta-blocker group vs 0.3 for the beta-blocker group; adjusted OR, 0.39 [95% CI, 0.16-0.96]). Results of propensity-matched analyses were broadly consistent.ConclusionsIn this study, the administration of beta-blockers before CABG was not associated with improved operative mortality and complications except the incidence of postoperative stroke in patients with left ventricular dysfunction. A more granular quality metric which would guide the use of beta-blockers should be developed.
Tooth Loss and Its Association With Frequency of Pregnancy: A Cross-Sectional Study Among Yemeni Women
Authors: Shamala Anas , Al-Maimooni Ebtesam , Al-Matari Salsbeel , Hiyat Ryhana , Al-wesabi Mohammed , Al-Maweri Sadeq , Nassani Mohammad , Halboub Esam 
Published: January 2021

Abstract
Backgrounds: The present study sought to assess tooth loss and oral hygiene practices among a sample of Yemeni women in association with giving births (parity).Subjects and Methods: This cross-sectional study included a sample of 644 Yemeni women. They were interviewed to gather socio-demographic (age, education, number of children) and behavioral data (oral hygiene practices, khat chewing and smoking). Additionally, they were examined clinically to assess number of tooth loss. Data were analyzed using SPSS with a P-value < 0.05 was considered statistically significant. Results: The participating women aged between 16 and 51 years. Only one fifth of the sample reported brushing their teeth regularly (i.e, at least once a day), and around 36.8% reported using dental aids occasionally. Additionally, 52% and 21% of the sample reported they were khat chewers and smokers, respectively. The mean number of tooth loss was 4.7. The mean number of children was 4.15, with approximately 54% of the participating women had more than 3 children. The results of the logistic regression revealed a significant association between number of children and tooth loss. Conclusion: Yemeni women had poor oral hygiene practices and high prevalence of tooth loss increased proportionally with the increased number of children. This emphasizes the importance of an effective oral hygiene motivation and health education among female patients during pregnancy periods.
Assessment of Safety and Efficacy of Ultra-micro Transconjunctival Lower Eyelid Bags Removal Combined with Orbital Fat Transfer
Authors: Wang Ke-xue , Xiang Xiong , Zhang Jian-feng , Ma Man-cang 
Published: January 2021

Abstract
BackgroundThe elaboration of a precise operation is essential during lower eyelid blepharoplasty. The aim of this study was to investigate the clinical effect of ultra-micro transconjunctival lower eyelid bags removal combined with orbital fat transfer in the correction of lower eyelid bags. MethodsA total of 226 patients underwent lower eyelid blepharoplasty in our department during the past 3 years (2018-2020). Excluded were patients with previous eyelid surgery, diplopia, strabismus and associated procedures. A retrospective chart review of patients undergoing traditional transconjunctival blepharoplasty (n=104) and ultra-micro transconjunctival lower eyelid bags removal combined with orbital fat transfer (n=122) was performed. The length of follow-up ranged between 24 hours and 3 months after operation. ResultsThe control group (conventional conjunctival blepharoplasty) included 45 males and 59 females with an average age of (44.5±3.5) years, while the observation group (subconjunctival blepharoplasty with orbital fat transfer) included 49 males and 73 females with an average age of (43.1±4.2) years. There were no statistically significant differences between the two groups in gender (p = 0.27) and age (p = 0.44) (p > 0.05). The Clinical improvement of the observation group is significantly higher than the control group, after 5 days has significant statistical differences between two groups (P < 0.05). It was found that the clinical efficiency of the observation group was significantly higher than that of the control group (P < 0.05). Next, we compared the complications between the two groups and found that there was a significant difference between the two groups (P < 0.05). The satisfaction of the two groups at different follow-up times was compared: 1 day after the operation, the satisfaction of the observation group was 52.5%, and that of the control group was 32.5%, with no statistically significant difference (P > 0.05). The satisfaction of the observation group was higher than that of the control group at 3d and 1 month after surgery, and the difference was statistically significant (P < 0.05).ConclusionThe application of ultra-micro transconjunctival lower eyelid bags removal combined with orbital fat transfer can correct the lower eyelid bag safely and effectively with short postoperative recovery time, high patient satisfaction and low complication rate. This advanced technical skill may significantly improve the treatment of lower eyelid bags.
Primary Splenic Histiocytic Sarcoma Associated with Hemophagocytic Lymphohistiocytosis (Hemophagocytic Syndrome): A Case Report of Next-Generation Sequency Involving&nbsp;FLT3, NOTCH2,&nbsp;and&nbsp;KMT2A&nbsp;Mutations
Authors: Montalvo Nelson , Lara Jorge , Redrobán Ligia , Leiva María , Armijos Christian , Russo Leonardo 
Published: January 2021

Abstract
BackgroundHistiocytic sarcoma (HS) is a very rare monocyte/macrophage-derived hematopoietic system tumor with a poor prognosis whose diagnosis is pathologically challenging due to its extreme rarity and histological overlap with various mimicking entities in which histiocytes also predominate.Case PresentationWe report the case of a 33-year-old male patient with hemophagocytic lymphohistiocytosis (HLH), purpuric syndrome, and significant splenomegaly. The patient underwent splenectomy; subsequent macroscopic examination revealed a spleen weighing 2,065 grams with hyperemic red pulp and multiple infarcts at the periphery. The histological and immunohistochemical study established a diagnosis of primary splenic histiocytic sarcoma (PSHS) with frequent hemophagocytosis. Next-generation sequencing (NGS) demonstrated mutations in FLT3, NOTCH2, and KMT2A, microsatellite stability (MSS), and a tumor mutational burden (TMB) of 2 mut/Mb. The patient's condition deteriorated clinically from the appearance of the first symptoms and he died six months later from multi-organ failure.ConclusionPrimary splenic histiocytic sarcoma (PSHS) is one of the rarest tumors of the hematopoietic system. We report the first case with mutations in FLT3, NOTCH2, and KMT2A, and associated hemophagocytic lymphohistiocytosis.
The Vaccine Coverage and Vaccine Immunity Status and Risk Factors of Non-Protective Levels of Antibodies Against Vaccines in Children with Juvenile Idiopathic Arthritis: Russian Tertiary Centre Study.
Authors: Kostik Mikhail , Lubimova Natalia A. , Fridman Irina V. , Goleva Olga V. , Kharit Susanna M. 
Published: January 2021

Abstract
Background: Immunosuppressive drugs, decreased vaccine coverage, aberrant immunity might be factors of low anti-vaccine antibodies in JIA patients. The study aimed to evaluate vaccine coverage, post-vaccine immunity and risk factors of non-protective levels of antibodies against measles, mumps, rubella, hepatitis B and diphtheria in JIA patients. Methods: A prospective study included 170 children diagnosed with JIA aged 2 to 17 years, who received routine vaccinations against measles, rubella, mumps (MMR) diphtheria and hepatitis B. In all patients, the levels of post-vaccination antibodies (IgG) for measles, rubella, mumps, hepatitis B and diphtheria measured with ELISA.Results: Protective level of antibodies were 50% against hepatitis B, 52% - diphtheria, 58% - measles, 80% - mumps, 98% rubella. The best coverage for MMR had patients with enthesytis-related arthritis-85%, compare to oligoarthritis-70%, polyarthritis-69%, systemic arthritis-63%. Diphtheria coverage was 50%, 51%, 46%, 63%, respectively. Incomplete MMR vaccination had 39% patients, treated with biologics, 22% with methotrexate and 14% with NSAID (p=0.025), and 61%, 46%, 36% for diphtheria (p=0.021). Incomplete vaccination was a risk factor of non-protective level of antibodies against measles (HR=2.03 [95%CI: 1.02; 4.0], p=0.042), parotitis (HR=6.25 [95%CI: 2.13; 17.9], p=0.0008) and diphtheria (HR=2.39 [95%CI: 1.18; 4.85], p=0.016) vaccines, as well as JIA category, biologics, corticosteroids and long-term methotrexate treatment for distinct vaccines.Conclusion: Children with JIA may have lower anti-vaccine antibodies levels and required routine check, especially in children with incomplete vaccination, biologics, systemic arthritis and long-term methotrexate treatment.
Attitude and Self Care Practice on Hypertension among Antihypertensive Medication Users in a Tertiary Care Hospital Nepal
Authors: Shrestha Jyoti , Sanhi Sabina , lamichhane Rajendra , Marasini Nabin Raj , Marasine Nirmal Raj 
Published: January 2021

Abstract
Background:Hypertension a “silent killer” is a serious global health problem, whose prevalence is increasing in Nepal. This study aimed to determine the attitude and practice of hypertension among antihypertensive medication users in a tertiary care teaching hospital in western Nepal.Methodology: A hospital-based cross-sectional study was conducted among 136 hypertensive patients under medication, aged ≥30 years, and visiting medical outpatient department, medical ward, and geriatric ward, using a non-probability convenience sampling technique. Semistructured questionnaires were used for data collection through interviews. Descriptive and inferential statistics were used, and a p-value < 0.05 was considered statistically significant.Results: The maximum number of patients (36; 26.5%) was in the age range of 50-60 years with a mean (SD) of 56.74 (12.58). Most of 77(56.6%) were male, Hindu, 95(69.9%), upper caste people (40; 29.4%), illiterate (30; 22.1%), house maker (37; 27.2), and monthly family income of 21,000 and above (66; 48.5).Almost half of the patients (69; 50.7%) had a positive attitude and more than half (71; 52.2%) had performed adequate self-care practice. Selected variables such as educational status and dietary pattern and attitude were significantly associated, whereas no association was found between sociodemographic variables and self-care practice. Attitude and self-care practices were found strongly associated with one another (p=0.002).Conclusion: Hypertensive patients were found with better level of attitude and adequate self-care practice. Educational interventions and awareness programs on dietary aspects should be focused for improving the attitude and practice of all the patient groups.
Use of a novel integrated port closure system - A multi-center study
Authors: Milad Qarawany , Mizrahi Hagar , Ugale Surendra , Pascal Guy , Hazzan David 
Published: January 2021

Abstract
BackgroundThe incidence of port site hernia (PSH) after laparoscopic surgery can reach up to 4 %. The fascial closure at the port site can be challenging in some cases.MethodsA multi-center, prospective, open label study to evaluate a new integrated port closure system (Gordian TroClose™ 1200 by Gordian Surgical™), is presented.ResultsFifty patients were enrolled in the study. All enrolled patients underwent laparoscopic surgery, during which at least one TroClose1200 device was used per patient. Patients were followed for two and six weeks and for one year. No port PSH related to TroClose 1200 device was diagnosed at one year of follow-up. One PSH was diagnosed at a non-TroClose1200 trocar site. The surgeons were very satisfied with the new device’s performance, with an average range of 4.8-5.0 out of 5.0 usability question being above 4.80 out of 5. No severe adverse events or device related adverse events were observed.ConclusionsThe TroClose 1200 is a simple, safe and friendly device that may reduce the incidence of port site hernia.
A Qualitative Study of Ghanaian Pediatric Oncology Nurses’ Care Practice Challenges.
Authors: Nukpezah Ruth Nimota , Fomani Fatemeh Khoshnavay , Hasanpour Marzieh , Nasrabadi Alireza Nikbakht 
Published: January 2021

Abstract
Background: Pediatric cancer is a global problem, and some studies have emphasized that nurses caring for these children experience work-related challenges. This has caused many children diagnosed with cancer to have a prolonged hospital stay and suffer unnecessary pain. However, there is insufficient documentary evidence on this issue. This study aims to explore and understand the challenges faced by pediatric oncology nurses in caring for children in Ghana.Methods: An exploratory qualitative research design study was conducted from August 2019 to April 2020. The study was conducted at the pediatric oncology unit which is located at the Tamale Teaching Hospital (TTH), Ghana. The study was conducted among 14 Ghanaian pediatric oncology nurses who were purposively sampled. A semi-structured interview guide was used to collect data. The interviews were recorded, transcribed verbatim, and analyzed inductively using Elo and Kyngas content analysis approach. The criteria proposed by Guba and Lincoln were used to ensure the validity of the study.Results: From the analysis of participants transcripts, eight subcategories emerged from two major categories. The subcategories were; time-consuming care, low job motivations, inadequate logistics, work stress, reduced labour force, low knowledge level, lack of teamwork and the perception of contracting cancer.Conclusions: The results point to several organizational and personal constraints experienced by the nurses who work at the pediatric oncology ward. It is hoped that by addressing these challenges, it would lead to further improvement in the care that is provided to children with cancer. There is the need for the administrative managers of hospitals, government and other stakeholders to invest in human, material and financial resources for delivering childhood cancer care services.
Modelling an unconventional closed-loop deep borehole heat exchanger (DBHE): Sensitivity Analysis on the Newberry Volcanic Setting
Authors: Doran Hannah Rose , Renaud Theo , Falcone Gioia , Pan Lehua , Verdin Patrick 
Published: January 2021

Abstract
Alternative (unconventional) deep geothermal designs are needed to provide a secure and efficient geothermal energy supply. An in-depth sensitivity analysis was investigated considering a deep borehole closed-loop heat exchanger (DBHE) to overcome the current limitations of deep EGS. A T2Well/EOS1 model previously calibrated on an experimental DBHE in Hawaii was adapted to the current NWG 55-29 well at the Newberry volcano site in Central Oregon. A sensitivity analysis was carried out, including parameters such as: the working fluid mass flow rate, the casing and cement thermal properties and the wellbore radii dimensions. The results conclude the highest energy flow rate to be 1.5MW, after an annulus radii increase and an imposed mass flow rate of 5kg/. At 3kg/s, the DBHE yielded an energy flow rate a factor of 3.5 lower than the NWG 55-29 conventional design. Despite this loss, the sensitivity analysis allows an assessment of the key thermodynamics within the wellbore and provide a valuable insight into how heat is lost/gained throughout the system. This analysis was performed under the assumption of subcritical conditions, and could aid the development of unconventional designs within future EGS work like the Newberry Deep Drilling Project (NDDP). Requirements for further software development are briefly discussed, which would facilitate the modelling of unconventional geothermal wells in supercritical systems to support EGS projects that could extend to deeper depths.
Maintaining proper health records improves machine learning predictions for novel 2019-nCoV
Authors: KHAN KOFFKA , Ramsahai Emilie 
Published: January 2021

Abstract
Background: An ongoing outbreak of a novel coronavirus (2019-nCoV) pneumonia continues to affect the whole world including major countries such as China, USA, Italy, France and the United Kingdom. We present outcome (’recovered’, ’isolated’ or ’death’) risk estimates of 2019-nCoV over ’early’ datasets. A major consideration is the likelihood of death for patients with 2019-nCoV.Method: Accounting for the impact of the variations in the reporting rate of 2019-nCoV, we used machine learning techniques (AdaBoost, bagging, extra-trees, decision trees and k-nearest Neighbour classifiers) on two 2019-nCoV datasets obtained from Kaggle on March 30, 2020. We used ’country’, ’age’ and ’gender’ as features to predict outcome for both datasets. We included the patient’s ’disease’ history (only present in the second dataset) to predict the outcome for the second dataset.Results: The use of a patient’s ’disease’ history improves the prediction of ’death’ by more than 7-fold. The models ignoring a patent’s ’disease’ history performed poorly in test predictions.Conclusion: Our findings indicate the potential of using a patient’s ’disease’ history as part of the feature set in machine learning techniques to improve 2019-nCoV predictions. This development can have a positive effect on predictive patient treatment and can result in easing currently overburdened healthcare systems worldwide, especially with the increasing prevalence of second and third wave re-infections in some countries.
Microbial fuel cell assisted utilization of glycerol for succinate production by mutant of Actinobacillus succinogenes
Authors: Zheng Tianwen , Xu Bin , Ji Yaliang , Zhang Wenming , Xin Fengxue , Dong Weiliang , Wei Ping , Ma Jiangfeng , Jiang Min 
Published: January 2021

Abstract
Background: The global production of glycerol is increasing year by year since the demands of biodiesel is rising. It is benefit for high-yield succinate synthesis due to its high reducing property. A. succinogenes, a succinate-producing candidate, cannot grow on glycerol anaerobically, as it needs a terminal electron acceptor to maintain the balance of intracellular NADH and NAD+. Microbial fuel cell (MFC) has been widely used to release extra intracellular electrons. However, A. succinogenes is a non-electroactive strain which need the support of electron shuttle in MFC, and pervious research showed that acid tolerant A. succinogenes has higher content of unsaturated fatty acids, which may be beneficial for the transmembrane transport of lipophilic electron shuttle.Results: MFC assisted succinate production was evaluated using neutral red as an electron shuttle to recover the glycerol utilization. Firstly, an acid tolerant mutant JF1315 was selected by atmospheric and room temperature plasma (ARTP) mutagenesis aiming to improve transmembrane transport of neutral red (NR). Additionally, MFC was established to increase the ratio of oxidized NR to reduced NR. By combining these two strategies, ability of JF1315 for glycerol utilization was significantly enhanced, and 23.92 g/L succinate was accumulated with a yield of 0.88 g/g from around 30 g/L initial glycerol, along with an output voltage above 300 mV.Conclusions: A novel MFC-assisted system was established to improve glycerol utilization by A. succinogenes for succinate and electricity production, making this system as a platform for chemicals production and electrical supply simultaneously.