bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Prognostic lncRNA Signature Associated with Lipid Metabolism in Glioma
Authors: Duan Weiwei , Li Chen , Cao Hui , Wen ZhiPeng , He Shiqing , Cheng Quan , Xia Zhiwei 
Published: June 2021

Abstract
Background: Long noncoding RNAs (lncRNAs) are promising cancer biomarkers and therapeutic targets . And lipid metabolism reprogramming is a trait of cancer metabolism. However, the relationship between lncRNAs and lipid metabolism and their prognostic value are still unclear in glioma. Hence, we screened for prognostic lncRNAs associated with lipid metabolism and explored their potential biological functions and effects on glioma. Methods: The glioma data were obtained from TCGA and CGGA databases. Correlation analysis was used to identify the lncRNAs associated with lipid metabolism . The Cox and LASSO regression analysis were adopted to find prognostic lncRNAs, and further established ceRNA network, clustering and risk score models. CNV and somatic mutation data were used to explore the correlation between risk score and genomic alterations. GSVA and GSEA was adopted to reveal the potential biological functions of prognostic lncRNAs. The abundance of 22 immune cells was inferred by CIBERSORT algorithm . The TIDE algorithm and GDSC database were used to predict the patient's response to immunotherapy and chemotherapy, respectively. Human glioma cell lines were used for further experimental validation. CCK-8 and colony forming assays were adopted to evaluate the cell viability. The cell proliferation was detected by EdU assay. Transwell assay was used to evaluate the ability of cell invasion and migration. Results: A total of twenty prognostic lncRNAs associated with lipid metabolism were found, and a prognostic lncRNA signature was established. A high risk-score suggested a poor prognosis , more malignant clinicopathological and genomic aberrations features. The risk score was also an independent prognos tic factor. The high-risk patients were more likely to benefit from anti-PD1 treatment. The biological function of signature lncRNAs was mainly to regulate the biosynthesis and transportation of lipid (especially the fatty acid). Among signature lncRNAs, LINC01614 was associated with prognosis, genomic aberrations characteristics, m6A methylation modification, immune infiltration status, and chemotherapy response of patients with glioma. In vitro experiments, silencing the expression of LINC01614 could inhibit the viability, migration , and proliferation of glioma cells. Conclusions: A prognostic lncRNA signature containing twenty lncRNAs associated with lipid metabolism was established in glioma. The signature lncRNAs play an important role in the development of glioma by regulating lipid biosynthesis and transportation. Our study also provides a new perspective for understanding the lipid metabolism and the biological role of lncRNAs in glioma.
Optimization of ultrasonically welded High density polyethylene with Medium density polyethylene
Authors: Asokan Vignesh , Prasanna Venkat 
Published: June 2021

Abstract
Ultrasonic welding is a mechanical system whereby high-recurrence ultrasonic acoustic vibrations are privately connected to workpieces being held together compelled to make a strong state weld. It is usually utilized for plastics, and particularly for joining disparate materials. The point of the undertaking is to examine the quality of weld when unique materials are welded together. The sort of weld will rely upon an on-hold time, weld time, weight, and recurrence. Materials utilized will be HDPE (High Density Polyethylene) and a blend of HDPE-LDPE which is termed as MDPE (Medium density polyethylene). Diverse materials have distinctive properties and thus making a joint with wanted properties will give a better outcome. If one material has properties like better elasticity, pressure quality and other material have properties like pliability and flexibility and so forth then the blend of these materials will have properties of both the materials and when these kinds of materials are welded ultrasonically, they give a better outcome. Subsequently we will get a material which will give better quality with ease. Likewise, the material will be increasingly adaptable.
Estimating the pre- and post-diagnosis costs of tuberculosis care for adults in Karachi, Pakistan: Household economic impact and strategies for meeting the costs
Authors: Razzaq Shama , Zahidie Aysha , Fatmi Zafar 
Published: June 2021

Abstract
Background

Despite of free TB care in Pakistan, patients still have to bear high costs which push them more into poverty. This study estimated the types of costs households bear for TB care, and coping mechanisms used for bearing TB expenditures among adults ≥ 18 years in Karachi, Pakistan.
Methods

A total of 516 TB patients with completion of at least one month intensive treatment were recruited from four public sector hospitals in Karachi, Pakistan. A standardized questionnaire to estimate patient's costs was administered. Direct medical and non-medical costs incurred as out-of-pocket and indirect costs (loss of income) during pre-diagnostic, diagnostic, treatment and hospitalization phase were estimated.
Results

Out of 516 participants, 52.1% were female with a mean age of 32.4 (± 13.7) years. The median costs per patient borne during the pre-diagnostic, diagnostic, treatment and hospitalization was estimated at USD63.8/ PKR7377, USD24/ PKR2755, USD10.5/ PKR1217 and USD349.0/ PKR40300, respectively. The total household median costs was estimated at USD129.2/ PKR14919 per patient. The median indirect costs were estimated at USD52.0/ PKR5950 per patient. First point of care was a private provider by 54.1% of patients, 36% attended public service, 5% and 4.1% went to dispensary and pharmacy, respectively.
Conclusion

TB patients bear substantial out-of-pocket costs before they are enrolled in publically funded TB program. There should be provision of transport and food vouchers, also health insurance for in-patient treatment. This advocates a critical investigation into an existing financial support network for TB patients in Pakistan with an eye towards easing the burden.
Design of decentralized adaptive control approach for large-scale nonlinear systems subjected to input delays under prescribed performance
Authors: Han Yu-Qun 
Published: June 2021

Abstract
For the first time, the issue of input delays and prescribed performance control is investigated in the same framework for large-scale nonlinear systems in this study, and a original adaptive decentralized control method is proposed take advantage of multi-dimensional Taylor network (MTN) method. Firstly, the problem of input delays is solved by introducing new variables, and a new form of coordinate transformation is introduced before controller design, which simplified the control system. Secondly, the problem of prescribed performance control is coped with by integrating the idea of prescribed performance into the Lyapunov functions of first step of backstepping of each subsystem. Thirdly, MTNs are employed to evaluate the combination of unknown functions, and then a decentralized MTN-based adaptive control scheme is developed by way of backstepping technology. The theoretical analysis indicates that the proposed control scheme can implement the expected tracking goals under the condition of meeting the prescribed performance control. Finally, one numerical example is given to show the validity and rationality of the proposed control method.
Exercise as an Add-on Strategy for the Treatment of Bipolar Disorder: Systematic Review and Meta-analysis Protocol
Authors: Rocca Andrey Rocha , Filho José Celio da Rocha Lima , Pesconi Rafael Barbosa Roque , Alves Déborah Alvim Monteiro Batista , Pereira João Victor Coutinho , Pontes Kaio Henrique Oliveira , Souza Pedro Vinicyus Novais , Ramos Marina Matos , Campos Rodolfo Nunes 
Published: June 2021

Abstract
Background: Bipolar disorder (BD) is a severe, recurrent and chronic disorder associated with cognitive impairment, reduction in quality of life and substantially reduction in psychosocial functioning. It presents high rates of comorbidity with cardiovascular and cerebrovascular diseases, diabetes and metabolic syndrome. Individuals with bipolar disorder need to focus their attention and treatment on mental and physical health. Physical exercise is often recommended in bipolar disorder, based on extrapolation from the major depressive disorder literature, theory and clinical expertise. However, studies tend to exclude individuals with BD or make no distinction between diagnostic groups, which leads to heterogeneity and difficulty in generalizing the results. The aim of this review is to evaluate the role of physical exercise as an intervention in bipolar disorder treatment. Method: The study populations must be humans, aged 18 years or older, with a clinical diagnosis of Bipolar Disorder (BD) according to a recognised widely-used diagnostic classification approach, confirmed with a structured interview. We will evaluate two main outcomes (mood symptoms improvement and functioning) and an additional outcome (prevention of relapse/recurrence). The search strategy will be based on the PICOS framework, using medical subject headings, on the following databases: MEDLINE (via Pubmed), EMBASE, CENTRAL, SPORTDiscus (via EBSCO), PsycINFO (via APA) and OpenGrey Repository. Selection and data collection process will be carried out by two authors, independently. Risk of bias and quality of evidence will be graded acording ROB-2 and GRADE. We will present a narrative and quantitative synthesis of the results from the included studies. Regarding quantitative data, we will extract means (M) and standard deviations (SD), when available, to calculate the standardised mean difference (SMD). Effects size will be calculated using SMD and 95% confidence interval and heterogeneity will be assessed. Subgroup analysis will be conducted to explore heterogeneity across studies depending on quality and quantity of the data extracted.Discussion: To date, there wasn't a systematic review with only randomized controlled trials on effects of physical activity on BD. Because of this, we will conduct this systematic review trying to estabilish the effects of exercise on mood, functionality and prevention of relapse.Registration: submitted
Community Dynamics of Fungi During the Progression of Human Non-Small-Cell Lung Cancer
Authors: Zhao Yiming , Yi Junqi , Xiang Juanjuan , Jia Wei , Chen Anqi , Chen Liyu , Zheng Leliang , Zhou Wen , Wu Minghua , Yu Zheng , Tang Jingqun 
Published: June 2021

Abstract
Background: Lung cancer is the most common cancer worldwide. The correlation between lung microbiota dysbiosis and lung cancer has been explored. However, the characteristics of the lung mycobiome in lung cancer patients has not been fully examined. In this study, we performed metagenomic sequencing on bronchoalveolar lavage (BAL) from 24 patients with non-small-cell lung cancer (NSCLC) and 14 non-cancerous controls with benign mass. Results: We observed greater fungal diversity and connectivity in NSCLC patients compared to non-cancer controls. Network analysis revealed that the sum of degrees in NSCLC was more numerous, suggesting the connections of fungal communities were more active. The community networks were reconstructed in 4 main ecological modules. The module and network hubs included Vanderwaltozyma polyspora, Apiotrichum porosum, Lobosporangium transversal, Capronia epimyces and Pseudomicrostroma glucosiphilum. Random forest analysis and DESeq2 were performed to screen out distinct species in two seperate groups and 3 fungi Alternaria arborescens, Eremoyces bilateralis and Malassezia restricta were identified as potential diagnosis biomarkers for NSCLC patients. In these 3 fungi, the relative abundance of Alternaria arborescens was gradually increasing as NSCLC progressed. Conclusions: Taken together, our study innovatively proposes that changes in the structure of fungal communities in lung cancer may be closely related to lung carcinogenesis as well as its progression and the distinct species listed above could be used to detect and monitor NSCLC.
Determinants of the Postprandial Triglyceride Response to a High-fat Meal in Healthy Overweight and Obese Adults.
Authors: Wilson Stephanie M , Maes Adam P. , Yeoman Carl J. , Walk Seth T. , Miles Mary P. 
Published: June 2021

Abstract
Background: Dyslipidemia is a feature of impaired metabolic health in conjunction with impaired glucose metabolism and central obesity. However, the contribution of factors to postprandial lipemia in healthy but metabolically at-risk adults is not well understood. We investigated the collective contribution of several physiologic and lifestyle factors to postprandial triglyceride (TG) response to a high-fat meal in healthy, overweight and obese adults. Methods: Overweight and obese adults (n=35) underwent a high-fat meal challenge with blood sampled at fasting and hourly in the 4-hour postprandial period after a breakfast containing 50 grams fat. Incremental area under the curve and postprandial magnitude for TG were calculated and data analyzed using a linear model with physiologic and lifestyle characteristics as explanatory variables. Model reduction was used to assess which explanatory variables contributed most to the postprandial TG response.Results: TG responses to a high-fat meal were variable between individuals, with approximately 57% of participants exceeded the nonfasting threshold for hypertriglyceridemia. Visceral adiposity was the strongest predictor of TG iAUC (β=0.53, p=0.01), followed by aerobic exercise frequency (β=0.31, p=0.05), insulin resistance based on HOMA-IR (β=0.30, p=0.04), and relative exercise intensity at which substrate utilization crossover occurred (β=0.05, p=0.04). For postprandial TG magnitude, visceral adiposity was a strong predictor (β=0.43, p<0.001) followed by aerobic exercise frequency (β=0.23, p=0.01), and exercise intensity for substrate utilization crossover (β=0.53, p=0.01). Conclusions: Postprandial TG responses to a high-fat meal was partially explained by several physiologic and lifestyle characteristics, including visceral adiposity, insulin resistance, aerobic exercise frequency, and relative substrate utilization crossover during exercise. Trial Registration: ClinicalTrials.gov, NCT04128839, Registered 16 October 2019 – Retrospectively registered, https://clinicaltrials.gov/ct2/show/NCT04128839
The Landing Error Scoring System Real-Time (LESS-RT) As a Predictive Tool for Overuse Injuries and Ankle Sprain: A Historical Cohort Study.
Authors: SCHWARTZ OREN , Talmy Tomer , Olsen Cara H , Dudkiewicz Israel 
Published: June 2021

Abstract
Background:Overuse injuries and ankle sprains are commonly sustained by soldiers and athletes during conditioning programs and may have significant ramifications on both individuals and organizations. The Landing Error Scoring System (LESS), and its expedited real-time version (LESS-RT) were developed to assess risk for ACL injuries, but have never been used to assess overuse or ankle injuries which are of high priority in a military or athletic settings. The purpose of this study was to evaluate predictive value of The Landing Error Scoring System Real-Time (LESS-RT) for overuse injuries of the lower limb and ankle sprains among Israel Defense Forces (IDF) combat soldiers.Methods: A historical cohort study was conducted on 2,474 IDF combat soldiers from different service units with a wide range of fitness levels and training requirements. The predictive variable was LESS-RT score and the outcome variables were the incidence of overuse injuries and ankle sprains. Receiver operator characteristic curves were used to assess the predictive value of LESS-RT for both outcomes. Results: The AUC for overuse injuries and ankle sprains were 0.537, 95% CI 0.514-0.560 and 0.523, 95% CI 0.491-0.556 respectively. Conclusions:The results of this study shows no predictive value of LESS-RT score for both overuse injuries of the lower limb and ankle sprains.
Examination of Patient Profiles Admitted to the Paediatric Emergency According to Various Variables
Authors: YUKSEL Ayfer 
Published: June 2021

Abstract
Purpose: The aim is to examine the applications to the paediatric emergency service of Foundation University Hospital in Ankara according to variables: gender, age, season of application, diagnosis, number of consultations and hospitalizations, and triage codes. Materials and methods: The research is a retrospective descriptive study. The data of patients admitted to the paediatric emergency department of a hospital in Ankara between 01.08.2017-31.07.2018 were obtained (n=16383). Frequency, percentages, cross table, and chi-square test were used for comparison. Findings: 0% of patients: very urgent application, 19%: urgent and 81%: non-urgent. The most common first three diagnoses: “unknown origin fever”, “cough”, "nausea and vomiting”. The 0-28 days age group had the lowest number of applications with 5% and had the highest emergency triage status with 66.5%; and the 19 months-5 years age group had the highest number of applications with 49% and had the lowest emergency triage rate with 12.8%.Conclusion: The reasons for applying to PES may differ according to contextual variables and providing parents with information from reliable sources may reduce unnecessary anxiety and emergency service admissions. There should be strengthened primary health care services and referral system to maintain high-quality emergency services.
An assessment of canine ectoparasiticide administration compliance in the United States based on timing of ectoparasiticide purchases recorded in veterinary hospital transactions.
Authors: Lavan Robert Philip , Normile Dorothy , Husain Imran , Singh Amita , Armstrong Robert , Heaney Kathleen 
Published: June 2021

Abstract
Background: This study evaluated the timing of dog owner ectoparasiticide purchases to estimate administration compliance and assess the consequent impact of dose purchase gaps on the proportion of time that dogs are protected over a 12-month period. Methods: Ectoparasiticide purchase transactions over a 12-month period were evaluated for dogs from 626 U.S. veterinary hospitals to determine dose purchase timing and identify consequent gaps between dose administration. Orally administered prescription ectoparasitic medications with active ingredients from the isoxazoline family (afoxolaner, fluralaner, lotilaner, or sarolaner) are included in the analysis. A period was calculated for each of the four isoxazoline-containing medications that represented the duration of protection provided by two doses of ectoparasiticide plus the average gap between these two doses. The maximum percentage of time possible for ectoparasiticide protection for this aggregate period was then calculated for each active. Results: Ectoparasiticide transaction records were analyzed for 506,637 dogs. Of these, 43% of dog owners purchased just one dose over the 12 months. If a dog owner purchased more than one dose, then the timing of these transactions could create a time gap between the completion of ectoparasite protection from the first dose and onset of protection from the subsequent purchase and administration of the second dose. Such gaps were observed in purchases made by 31-65% of dog owners depending on the selected active ingredient and number of doses. The average gap duration between dose purchases was calculated for all possible dose combinations over 12 months of ectoparasite protection. Time gaps between the first and second doses are as follows: for sarolaner (20.3 weeks), afoxolaner (12.9 weeks), fluralaner (12.8 weeks), and lotilaner (8.9 weeks). The proportion of time when protection was provided during the aggregate period between administration of the first and second doses was fluralaner 65%, lotilaner 49%, afoxolaner 40,% and sarolaner 30%. Conclusions: Dog owner ectoparasiticide purchase transactions show that there are time gaps between doses leading to reduced ectoparasite protection. The longer re-administration interval of fluralaner, which results from its extended duration, results in dog owners gaining the greatest proportion of ectoparasite protection time compared with shorter-acting monthly re-treatment medications.
Diffuse Midline Glioma with H3-K27M Mutation: A Rare Case with GFAP-positive Anucleate Whorled Patterns
Authors: Wang Yi-Hua , Gu Jian , Yu Juan-Han , Fu Lin , Li Qing-Chang , Qiu Xue-Shan , Wang En-Hua 
Published: June 2021

Abstract
Background: Diffuse midline glioma with H3-K27M mutation is a new tumor type in the 2016 WHO classification of tumors of the central nervous system, with predominantly astrocytic differentiation and is characterized by K27M mutation in either H3F3A/-HIST1H3B/C. It occurs at the midline of the CNS. It is mainly found in children, and adult cases are relatively rare . Although its histopathological features have been widely described, GFAP-positive anucleate whorled patterns have not been reported to the best of our knowledge. Hence, we have reported this unusual case.Case presentation：A woman presented with complaints of walking instability. MRI showed a mass shadow of isometric T1 and slightly longer T2 with mild mixed signals in the third ventricle of the suprasellar region, about 3.4 x 2.5 x 3.4 cm in size. The enhanced scan showed that the lesion was irregular and annular, with a clear edge. The lesion had spread to the right lateral ventricle, which dilated the ventricular system, especially the right lateral ventricle. Furthermore, the lamellar long T2 signal was seen in bilateral paraventricular white matter, the midline structure had shifted to the left, and the corpus callosum was thinner. Radiological findings suggested ependymoma. Subtotal resection craniotomy was performed. After surgery, the patient was routinely treated with temozolomide for chemotherapy and synchronous radiotherapy (Dt=60 Gy/30f). It has been 11 months now, and the patient is living well.Result: We described an extremely rare case of diffuse midline glioma, H3-K27M mutation with GFAP-positive anucleate whorled patterns. Conclusions: This case report provides information on the microscopic morphological features of diffuse midline glioma with H3K27M mutation, which can help pathologists to make a definitive diagnosis of this tumor.
CircFNDC3B inhibits cell growth in abdominal aortic aneurysm by targeting the miR-1270/PDCD10 axis
Authors: Deng Baoping , Wei Yue , Zhang Jinfeng , Zeng Na , He Yulan , Zeng Qiaoli , Zou Dehua , Guo Runmin , Xu Jing 
Published: June 2021

Abstract
BackgroudIn certain cancers, circRNA fibronectin type III domain containing 3B (circFNDC3B) may serve as a specific target for the treatment. However, the role and underlying regulatory mechanisms of circFNDC3B in abdominal aortic aneurysm (AAA) remain unknown.MaterialsCircFNDC3B expression in AAA and normal tissues were assessed by qRT-PCR. The biological functions of circFNDC3B were evaluated by MTT, flow cytometry and Caspase-3 activity assays. Furthermore, the molecular mechanism of circFNDC3B was demonstrated by RNA immunoprecipitation (RIP), dual-luciferase reporter assay, western blotting, qRT-PCR and rescue experiments. ResultsWe found that the expression of circFNDC3B was significantly upregulated in AAA clinical specimens. Functionally, overexpression of circFNDC3B inhibited vascular smooth muscle cell (VSMC) proliferation and induced apoptosis in vitro, yet, knockdown of circFNDC3B had the opposite effects. Mechanistically, circFNDC3B upregulated the expression of programmed cell death 10 (PDCD10) by acting as a molecular sponge for miR-1270. Notably, forced expression of PDCD10 countervailed the impact of circFNDC3B knockdown on AAA biological processes.ConclusionsOur data indicated that circFNDC3B promoted the progression of AAA by targeting the miR-1270/PDCD10 pathway, and may be a potential therapeutic target in the treatment of AAA.