bluuebunny/crossref_metadata_2025_split huggingface dataset:
Crustal Deformation Detection Capability Associated With a Fault Slip on the Interplate Boundary in the Gnss-a Seafloor Geodetic Observation Array (SGO-A), Provided By Japan Coast Guard
Authors: Yokota Yusuke , Ishikawa Tadashi , Watanabe Sun-ichi , Nakamura Yuto 
Published: August 2021

Abstract
The GNSS-A technique is an observation method that can detect seafloor crustal deformations with centimeter level accuracy. The GNSS-A seafloor geodetic observation array operated by the Japan Coast Guard, called SGO-A, has been constructed near the Japan Islands along the Nankai Trough and the Japan Trench. This observation array has detected several earthquakes’ displacements and episodic slow crustal deformation. To compare the detection results of SGO-A with other observation networks and expand the SGO-A distribution, it is necessary to correctly understand its detection capability. In this paper, the capabilities of current GNSS-A (frequency: f = 4–6 times/year, position accuracy: σ (standard deviation) = 1.5 cm) to detect a crustal deformation rate only, an event only, and crustal deformation rate and event together were arranged by numerical simulations. Results suggested the following features: when it is known that there is no event, the 95% confidence level (CL) for the estimation of crustal deformation rate with 4-year observation is about 0.5–0.8 cm/year; when the deformation rate is known, a signal of about 3.0 cm can be detected by observations of about 4 times before and after the event. When the deformation rate and the event are detected together, to keep the false positive low (about 0.05), the false negative becomes high (about 0.2–0.7 for detecting a signal of 4.5–6.0 cm). The determined rate and event variations are approximately 1.8 cm/year (95%CL) and 1.5 cm (standard deviation), respectively. We also examined the detection capability for higher frequency and accuracy, to examine how the detection capability improves by technological advancements in the future. Additionally, we calculated the spatial range of event detectability using the determined values of detection sensitivity. Each seafloor site can detect a slip event larger than 0.1 m scale within about 50 km radius. A subseafloor slip event smaller than about 1 m at the distance of 100 km or more from the land can often be detected only on the seafloor observation array.
Establishment of a Novel CD34+ Hematopoietic Stem Cell-Derived Xenograft Model of Hyperleukocytic Acute Myeloid Leukemia
Authors: Jin Yanxia , Luo Ziyi , Ma Linlu , Chen Yanling , Zheng Yujia , Liang Yuxing , Tong Xiqin , Yang Qian , Wu Balu , Wu Sanyun , Shen Hui , Liu Li , Liu Yin , Yang Tian , Zhou Fuling 
Published: August 2021

Abstract
Hyperleukocytic acute myeloid leukemia (HLL) with high early mortality is difficult to therapy. The related research on HLL is still in its infancy, and the establishment of a patient derived xenograft (PDX) model of HLL has not been fully reported. In this study, we establish a CD34+ hematopoietic stem cell-derived xenograft model of HLL for further exploration. Our findings demonstrated that the leukemia cells were detected in peripheral blood, bone marrow, liver, and spleen tissues in mice after inoculation of patient-derived cells. By performing a comparative analysis of multiple experiments, we found the irradiation doses, injection cell counts, gene abnormal and the survival status of the enrolled patients affected the survival time in mice. We successfully established a novel CD34+ hematopoietic stem cell-derived xenograft model of HLL, which shows great promise for mechanistic research, drug screening, individualized therapy, clinical efficacy assessment and precision medicine in HLL.
WITHDRAWN: MicroRNA-155-5p Contributes to 5-Fluorouracil Resistance Through Down-Regulating TP53INP1 in Oral Squamous Cell Carcinoma
Authors: Liu Bowen , Hu Jingchao , Zhao Han , Zhao Li 
Published: August 2021

Abstract
The anticancer drug 5-fluorouracil (5-FU) resistance is a major obstacle to reducing the effectiveness of cancer treatment, and its detailed mechanism has not been fully elucidated. Here, in 5-FU-resistant human oral squamous cell carcinoma (OSCC) HSC3 cells (HSC3/5-FU), the levels of 21 miRNA candidates were detected and miR-155-5p level increased strikingly in HSC3/5-FU cells compared to HSC3 cells. Compared with HSC3 cells, the HSC3/5-FU cells transfected with miR-155-5p inhibitors decreased 5-FU IC50. Ectopic expression of miR-155-5p in HSC3 and HSC4 cells increased 5-FU IC50, migration and invasion abilities. Seven miR-155-5p target candidates were discovered by miRNA prediction algorithms, and in HSC3/5-FU cells TP53INP1 showed the lowest mRNA expression level compared with HSC3 cells. Ectopic expression of miR-155-5p in HSC3 and HSC4 cells decreased TP53INP1 expression level, and luciferase reporter assay further determined the interference effect of miR-155-5p on TP53INP1 expression. The enhancement of cell viability, migration and invasion by miR-155-5p after 5-FU treatment was reversed by TP53INP1 overexpression. After treatment with 5-FU, HSC3-miR-155-5p tumor-bearing nude mice presented growing tumors, while HSC3-TP53INP1 group possessed shrinking tumors. In conclusion, these results lead to the proposal that miR-155-5p enhances 5-FU resistance by decreasing TP53INP1 expression in OSCC.
Comparative Genetics Using Three mtDNA Markers in Aedes aegypti (Linnaeus) Populations from Four Municipalities in the Satate of Mato Grosso, Brazil.
Authors: Mariotto Sandra , Silva Mari Rose Oliveira , Butakka Cristina Márcia Menezes , Cerqueira Lenicy Lucas Miranda , Miyazaki Rosina Djunko , Rodrigues Fabiana Aparecida Caldart 
Published: August 2021

Abstract
Aedes aegypti mosquito has spread throughout the tropical and subtropical world and is currently the primary species responsible for transmitting dengue, urban yellow fever, Chikungunya, and Zika virus. This study aimed to investigate the inter- and intrapopulational genetic variability of Aedes aegypti through mitochondrial DNA, COI, ND4, and ND5 molecular markers in four municipalities in Mato Grosso. We used the Geneious software to build dendrograms for differentiating populations from each municipality. The interpopulational genetic distance obtained from sequence analysis showed a difference within populations through groups' formation in the ordering. Besides, we identified a difference in the interindividual genetic distance values, notably for the ND5 gene from the populations captured in the four municipalities. We recorded the smallest interindividual genetic distance within populations for populations from Chapada dos Guimarães. Extrinsic factors, including breeding habitat removal, can contribute to decreasing variability. Consequently, the dendrogram showed some similarities. Ovitrap monitoring, vector elimination, and genetic flow investigation stimulate actions to prevent transmitted diseases and support essential effective measures to control and fight Ae. aegypti.
CELF1 Promotes Matrix Metalloproteinases Gene Expression at Transcriptional Level in Lens Epithelial Cells
Authors: Xiao Jun , Tian Xi , Jin Siyan , He Yanhui , Song Meijiao , Zou He 
Published: August 2021

Abstract
Background: RNA binding proteins (RBPs)-mediated regulation plays important roles in many eye diseases, including the canonical RBP CELF1 in cataract. While the definite molecular regulatory mechanisms of CELF1 on cataract still remain elusive. Methods: In this study, we overexpressed CELF1 in lens epithelial SRA01/04 cells and applied whole transcriptome sequencing (RNA-seq) method to analyze the global differences mediated by CELF1. We then analyzed public RNA-seq and CELF1-RNA interactome data to decipher the underlying mechanisms.Results: The results showed that transcriptome profile was globally changed by CELF1 overexpression (CELF1-OE). Functional analysis revealed CELF1 specifically increased the expression of genes in extracellular matrix disassembly, extracellular matrix organization, and proteolysis, which could be classified into matrix metalloproteinases (MMPs) family. This finding was also validated by RT-qPCR and public mouse early embryonic lens data. Integrating analysis with public CELF1-RNA interactome data revealed that no obvious CELF1-binding peak was found on the transcripts of these genes, indicating an indirectly regulatory role of CELF1 in lens epithelial cells. Conclusions: Our study demonstrated that CELF1-OE promotes transcriptional level of MMP genes; and this regulation may be completed by other ways except for binding to RNA targets. These results suggest that CELF1-OE is implicated in the development of lens, which is associated with cataract and expands our understanding of CELF1 regulatory roles as an RNA binding protein.
Guided Endodontics: Accuracy of Access Cavity Preparation and Discrimination of Angular and Linear Deviation On Canal Accessing Ability – An Ex Vivo Study
Authors: Su Ying-Hui , Chen Cheng-Hui , Lin Chia-Hua , Lee Hui-Na , Chen Ker-Kong , Lin Yen-Kun , Chuang Fu-Hsiung 
Published: August 2021

Abstract
Background
Guided endodontics technique has been introduced for years, but the accuracy in different types of teeth has yet to be assessed. The aim of this study is to evaluate the accuracy of three dimensional (3D)-printed endodontic guides for access cavity preparation in different types of teeth, and to evaluate the predictive ability of angular and linear deviation on canal accessibility ex vivo.
Method
Eighty-four extracted human teeth were mounted into six jaw models and categorised into three groups: anterior teeth (AT), premolar (P), and molar (M). Preoperative cone beam computed tomography (CBCT) and surface scans were taken and matched using implant planning software. Virtual access cavity planning was performed, and templates were produced using a 3D printer. After access cavities were performed, the canal accessibility was recorded. Postoperative CBCT scans were superimposed in software. Coronal and apical linear deviations and angular deviations were measured and evaluated with nonparametric statistics. The receiver operating characteristic (ROC) curve was used to evaluate the predictive ability of angular and linear deviation for canal accessibility in SPSS v20.
Results
A total of 117 guided access cavities were created and 23 of them were record as canal inaccessibility, but all canals were accessible after canal negotiation. The average linear deviation for all groups was 0.13 ± 0.21 mm at coronal position, 0.46 ± 0.4 mm at apical position, and 2.8 ± 2.6° in angular deviation. At the coronal position, the linear deviations of the AT and P groups were significantly lower than M group deviation (P < 0.05), but no statistically significant difference between AT group and P group. The same results were found in linear deviation at the apical position and in angular deviation. The area under the ROC curve was 0.975 in angular deviation, 0.562 in linear deviation at the coronal position, and 0.786 at the apical position. Statistical significance was noted in linear deviation at the apical position and in angular deviation (P < 0.001).
Conclusions
In conclusion, this study demonstrated that the accuracy of access cavity preparation with 3D-printed endodontic guides was acceptable. The linear and angular deviations in the M group were significantly higher than those in the other groups, which might be caused by the interference of the opposite teeth. Angular deviation best discriminated the canal access ability of guided access cavity preparation.
Investigating Closed Room Conspirators of Posttraumatic Inflammatory Response: Multi- Omics Profiles of Exosomes Derived from Intestinal Epithelial Cells Under Ischemia Reperfusion Injury
Authors: Senda Atsushi , Kojima Mitsuaki , Watanabe Arisa , Kobayashi Tetsuyuki , Nakatsutsumi Keita , Yagi Masayuki , Morishita Koji , Aiboshi Junichi , Otomo Yasuhiro 
Published: August 2021

Abstract
Intestinal ischemia-reperfusion injury leads to multiple organ injuries via gut-derived mediators following severe injury. Growing evidence suggests that exosomes secreted from intestinal epithelial cells are heavily involved in the development of systemic inflammation, but a full elucidation of its pathology remains to be completed. To produce an integrated understanding of its pathology, this study aimed to reveal the changes in exosome content after ischemic stimulation. Our result showed (1) the proteins involved in inflammation by catalyzing RNAs were upregulated, (2) hsa-miR-21-5p, hsa-miR-23a-3p, and hsa-miR-30d-5p levels were increased while hsa-miR-124-3p level was decreased, (3) the increase in unsaturated lysophosphatidylcholines levels. These results together with those of previous studies, suggest that lysophosphatidylcholines may activate the NK-κB pathway. The proteins and microRNAs jointly act to disrupt negative feedback, thereby increasing inflammation. Thus, our results clarify part of the mechanism of multi-organ failure after intestinal ischemic recanalization, thereby providing a new target for treatment.
The Effects of Total Parenteral Nutrition on Plasma Aluminium Level in Premature
Authors: bahar SEMRA , zubarioglu umut , Bulbul Ali 
Published: August 2021

Abstract
There are studies showing that parenteral nutrition fluids used for adaptation of preterm infants contain aluminum. It is known that aluminum accumulates in the body, disrupts mental development, affects bone mineral structure. In our study, we aimed to investigate parenteral nutrition and aluminum transmission in preterm infants. A total of 45 babies born during pregnancy week ≤32 and / or under 1500 gr were included in the study. A statistically significant increase was observed when we compared the levels of aluminum in the cord blood and in the 14th day of the patients. When the factors affecting serum aluminum levels were examined, no significant difference was found between birth weight, birth week and gender. No statistically significant difference was found between the duration of taking antibiotics, inotropic drugs serum aluminum levels. Statistically significance was found between number of days of furosemid administeration and serum Al levels. Longer parenteral nutrition was associated with greater increase in serum aluminium levels. A significant positive correlation was found between serum aluminium levels on 14th day and the calcium and magnesium levels in the TPN solutions which were administered in the first 7 days. In conclusion, the increase in serum aluminum levels in preterm infants fed parenteral nutrition was found to be statistically significant. In infants with longer parenteral nutrition, the increase in serum aluminum levels was greater. It is planned to continue our research in terms of long term effects of aluminum.
Androgen regulates the expression and function of connexin 43 in granulosa cells via the PKC signalling pathway
Authors: Zhang Yangyang , Xu Yang , Kuai Yanrong , Wang Sheng , Yu Jiao 
Published: August 2021

Abstract
Background

Poor ovarian response (POR) is one of the most challenging issues in assisted reproductive technology. Given the importance of androgens in follicle recruitment, growth and development, domestic and foreign reproductive centers have added androgen as an adjuvant to in vitro fertilization protocols for patients with POR to improve the ovarian response. The mechanism underlying the effects of androgens is unclear. The aim of this research was to study the effects of testosterone on granulosa cells and the underlying mechanisms.
Methods

The human ovarian granulosa cell line KGN was treated with testosterone at various concentrations, an androgen receptor antagonist (flutamide) and a protein kinase C (PKC) inhibitor (GF109203X). The protein levels of PKC and phosphorylated connexin 43 (p-Cx43) were measured using Western blot analysis. The location and distribution of Cx43 and p-Cx43 were determined by immunofluorescence. Gap junction intercellular communication (GJIC) was monitored using the scrape loading/dye transfer method.
Results

The results showed that treatment with testosterone at a physiological level significantly increased the PKC and p-Cx43 levels and GJIC activity in a concentration-dependent manner. However, the expression of PKC, p-Cx43 levels and GJIC activity in the cells treated with testosterone above physiological concentrations (10− 5 M) decreased compared with those in the cells treated with 10− 7 M testosterone. Compared to the 10− 7 M testosterone-treated cells, the KGN cells treated with flutamide had much lower levels of PKC and p-Cx43. The levels of p-Cx43 and GJIC activity in the GF109203X-treated group were decreased compared to those in the 10− 7 M testosterone group. Immunofluorescence showed that testosterone treatment had no effect on the localization and distribution of gap junction proteins.
Conclusions

This study suggests that androgen can increase GJIC activity in granulosa cells by phosphorylating Cx43 via the PKC signalling pathway to promote oocyte development. The results of this study might provide a theoretical basis for androgen pretreatment of patients with POR.
Non-Field Analytical Method for Filtration of Slightly Compressible Fluid through Porous Media with Stagnation Areas
Authors: Kulish Vladimir , Schmirler Michal , Sláma Pavel 
Published: August 2021

Abstract
In this study the method of Kulish has been used to derive a non-field solution of the equation, which models the process of unsteady filtration of a slightly compressible fluid within a domain consisting of both flow and stagnation areas under the influence of some pressure distribution at the boundary. The solution relates the local values of pressure and the corresponding pressure gradient and is valid everywhere within the domain including the boundary. The solution thus obtained is in the form of a series with respect to generalised differ-integral operators of fractional orders. The solution has been compared with the know solution of the filtration problem with no stagnation areas. Finally, an integral equation to estimate the pressure evolution at the boundary for a given filtration speed has been proposed.
Development of Hybrid Artificial Neural Network and Decision Tree Algorithm for Air Quality Prediction
Authors: Gunasekar S , Kumar G Joselin Retna , Vijayakumar K , Agbulu G Pius 
Published: August 2021

Abstract
With the drastic development of smart cities and technology, various pollutions occurred in an environment such as air, water and noise. More specifically, air pollution has risen due to the population and transportation and it also direct impact on human health. To suggest a useful preventive measure, proper prediction of air quality is needed. Today machine learning (ML) algorithms support intelligent data analysis and are applied in various fields like medical, Market basket analysis, Finance and weather forecasting etc. This work is used to analyze the performance of air quality prediction by using various ML algorithms. In addition, a hybrid neural network and decision tree model is proposed for accurate forecasting. Experiential results show that the proposed hybrid model achieves higher accuracy than other methods. The proposed model attained an accuracy of 99.88%, with a sensitivity score of 99% and a specificity score of 99.88%.
Influence of intensive parameters and assemblies on friction evolution during piston-cylinder experiments
Authors: Condamine Pierre , Tournier Simon , Charlier Bernard , Médard Etienne , Triantafyllou Antoine , Dalou Célia , Tissandier Laurent , Lequin Delphine , Cartier Camille , Füri Evelyn , Burnard Pete G. , Demouchy Sylvie , Marrocchi Yves 
Published: August 2021

Abstract
Piston-cylinder assemblies exhibit inhomogeneous pressure distributions and biases compared to the theoretical pressure applied to the hydraulic press because of the thermal and mechanical properties of the assembly components. Whereas these effects can partially be corrected by conventional calibration, systematic quantification of friction values remain very sparse and results vary greatly among previous studies. We performed an experimental study to investigate the behavior of the most common cell assemblies, i.e., talc [Mg3Si4O10(OH)2], NaCl, and BaCO3, during piston-cylinder experiments to estimate the effects of pressure, temperature, run duration, assembly size, and assembly materials on friction values. Our study demonstrates that friction decreases with time and also partially depends on temperature but does not depend on pressure. We determined that friction decreases from 24 to 17% as temperature increases from 900 to 1300 °C when using talc cells, indicating a friction decrease of ~2% per 100 °C increase for 24 h experiments. In contrast, friction becomes independent of time above 1300 °C. Moreover, at a fixed temperature of 900 °C, friction decreases from 29% in 6 h runs to 21% in 48 h runs, corresponding to a decrease of friction of 0.2% per hour. Similar results obtained with NaCl cell assemblies suggest that friction is constant within error, from 8% in 9 h runs to 5% in 24 h runs. At 900 °C, possible steady-state friction values are only reached after at least 48 h, indicating that friction should be considered a variable for shorter experiments. We establish that assembly materials (and their associated thermomechanical properties) influence the friction correction more than the dimensions of the assembly parts. Finally, we show that the use of polytetrafluoroethylene film instead of conventional Pb foil does not modify friction but significantly reduces the force required for sample extraction, thus increasing the lifetime of the carbide core, which in turn enhances experimental reproducibility.
Efficacy and safety of carotid endarterectomy in the acute phase of ischemic stroke
Authors: Khayrutdinov A. I. , Tarasov Yu. V. , Valiullin D. Kh. , Yakubova V. T. , Komarov R. N. , Shchanitsyn I. N. , Bazhanov S. P. 
Published: August 2021

Introduction.Nowadays it’s recommended to perform carotid endarterectomy (CEA) in up to 14 days after nondisabling stroke; the procedure is aimed at the prevention of recurrent stroke.The objectiveof this research was the comparison of short-term and long-term (12 months) outcomes with early (in up to 30 days) and delayed (30-180 days) CEA in patients who suffered strokes of various severities.Materials and methods. The research involved 88 patients who underwent CEA in the early stage (Group 1) and 88 patients who underwent CEA in their late period (Group 2). We assessed primary endpoints: ipsilateral stroke, myocardial infarction, 30-day Lethality, 30-day lethality after the surgery, any stroke or infarction (MACE). Secondary endpoints: the same parameters within 12 months after the surgery, post-surgery local or systemic complications, restenosis, changes in neurologic or cognitive status.Results.In 30 days we observed ipsilateral strokes in 3 patients in Group 1 (3.4 percent), it was lethal in 1 patient (1.1 percent). 170 of 176 (96 percent) patients were followed up for 12 months. One lethal stroke was registered in the group of late interventions (1.1 percent). No statistically significant differences were observed between the outcomes in early and late treatment groups. The severity of disability (mRS) in patients of the early intervention group was significantly smaller at discharge and in 12 months after surgery.Conclusion.The advisability of early CEA performance was proven by the absence of differences in post-surgery 30-day and longterm lethality as well as the progress of stroke or infarction with early or late CEA. In 12 months after the surgeries, significant improvement in neurologic status by mRS was only observed in the group of early interventions.