bluuebunny/crossref_metadata_2025_split huggingface dataset:
Quantifying the effects of varietal types × management on the spatial variability of sorghum biomass across US environments
Authors: Ojeda Jonathan J. , Hammer Graeme , Yang Kai‐Wei , Tuinstra Mitchell R. , deVoil Peter , McLean Greg , Huber Isaiah , Volenec Jeffrey J. , Brouder Sylvie M. , Archontoulis Sotirios , Chapman Scott C. 
Published: December 2021

AbstractRegional‐scale estimations of sorghum biomass production allow identification of optimum genotype×environment×management (G×E×M) combinations for bioenergy generation. The objective of this study was to determine the degree of contributions of G, E, and M toward variability in sorghum biomass in the United States. Using the Agricultural Production Systems sIMulator in a grid computing platform, biomass was simulated for irrigated and rainfed conditions for 30 years across the United States for four sorghum varietal types (grain—GS, sudangrass—SS, photosensitive—PS, and photo‐insensitive—PI). Simulated biomass was assessed by environments clustered using the sum of intercepted solar radiation (ir), mean of temperature stress factor (tp) and water stress factor (sw). Simulated biomass ranged from 5.8 t ha−1 (GS‐rainfed) to 27.5 t ha−1 (PI‐irrigated). Under high‐temperature environments (mean annual temperature = 25°C), rainfed biomass between 40 and 80 days after planting (DAP) was strongly correlated with sw (r = 0.64–0.86) and irrigated biomass with ir (r = 0.68–0.81). Under low‐temperature environments (mean annual temperature = 18°C) after 40 DAP, tp and ir had greater effects than sw (r = 0.55–0.82). Biomass variance was mainly explained by varietal type (50%–76%) in all environments×irrigation combinations, except in the high‐ and mid‐temperature environments under rainfed conditions where rainfall had the major effect (25%–45%). However, when mean temperature during the growing season decreased from 25°C (high environments) to 18°C (low environments), the contribution of mean temperature to biomass variance increased from 7% to 34% (rainfed) and from 4% to 36% (irrigated). Varietal type had the larger interactions with other factors independently of the environment and irrigation. We demonstrated a need to quantify (i) the main G×E×M drivers of biomass variability based on environmental stress factors and (ii) the variance contribution of these drivers on sorghum biomass. Our regional‐scale estimations are key inputs for future robust biomass projections of energy sorghum genotypes integrating G×E×M under climate change scenarios.
Human amniotic membrane extracellular matrix scaffold for dental pulp regeneration in vitro and in vivo
Authors: Bakhtiar Hengameh , Ashoori Azin , Rajabi Sarah , Pezeshki‐Modaress Mohamad , Ayati Alireza , Mousavi Mohammad Reza , Ellini Mohammad Reza , Kamali Amir , Azarpazhooh Amir , Kishen Anil 
Published: December 2021

AbstractAimIn order to obtain a 3‐dimentional scaffold with predictable clinical results for pulp regeneration, this study aims to fabricate and characterize a porous decellularized human amniotic membrane (HAM) extracellular matrix (ECM) scaffold, and evaluate its potential to promote pulp regeneration in vitro and in vivo.MethodologyThe HAM was decellularized, and its histology and DNA content were analysed to confirm decellularization. The scaffolds were synthesized with 15, 22.5 and 30 mg/ml concentrations. The porosity, pore size, phosphate‐buffered saline (PBS) absorption and degradation rate of the scaffolds were assessed. In vitro experiments were performed on human dental pulp stem cells (hDPSCs) to assess their viability, proliferation, adhesion and migration on the scaffolds. The optimal group was selected for in vivo immunogenicity assessment and was also used as the cell‐free or cell‐loaded scaffold in root segment models to evaluate pulp regeneration. All nonparametric data were analysed with the Kruskal–Wallis test followed by Dunn's post hoc test, whilst quantitative data were analysed with one‐way anova.ResultsDecellularization of HAM was confirmed (p < .05). The porosity of all scaffolds was more than 95%, and the pore size decreased with an increase in ECM concentration (p < .01). PBS absorption was not significantly different amongst the groups, whilst 30 mg/ml ECM scaffold had the highest degradation rate (p < .01). The hDPSCs adhered to the scaffold, whilst their proliferation rate increased over time in all groups (p < .001). Cell migration was higher in 30 mg/ml ECM scaffold (p < .05). In vivo investigation with 30 mg/ml ECM scaffold revealed mild to moderate inflammatory response. In root segments, both cell‐free and cell‐loaded 30 mg/ml scaffolds were replaced with newly formed, pulp‐like tissue with no significant difference between groups. Immunohistochemical assessments revealed high revascularization and collagen content with no significant difference amongst the groups.ConclusionThe 30 mg/ml HAM ECM scaffold had optimal physical properties and better supported hDPSC migration. The HAM ECM scaffold did not interfere with formation of pulp‐like tissue and revascularization within the root canal when employed as both cell‐free and cell‐loaded scaffold. These results highlight the potential of HAM ECM membrane for further investigations in regenerative endodontics.
Consumer collecting behaviour: A systematic review and future research agenda
Authors: Lee Cary , Brennan Stacey , Wyllie Jessica 
Published: December 2021

AbstractConsumer interest in collecting is surging globally, with the behaviour prevalent as a hobby and for investment. Additionally, collecting is technologically advancing and continues to infiltrate regular shopping as a potent sales promotion tool, presenting marketing and ethical implications. Despite collecting's burgeoning popularity in practice, research is lagging. To address this limitation, the present study conducts a systematic review, consolidating over 30 years of research into the motivations that drive consumer collecting. Following the format of a domain‐based structured systematic literature review, the study provides scholars theoretical, methodological, and thematic guidance. Our analysis of the 51 journal articles reveals a set of six motivations driving the consumption of collectibles, including Achievement through Collecting Goals; Social Membership; Cooperation and Competition; Societal and Personal Memories; Legacy; and Financial Value. The findings from the review also illuminate several shortcomings in the literature and the complexity associated with the theoretical grounding of consumer collecting research and of its associated behaviours (i.e., acquisition through disposal). Future research propositions are presented in the Theory, Context, and Methodology framework that provides direction for the scholarly community and aids further theory development in consumer collecting research.
Unravelling the relation between natural microbiota and biogenic amines in Brazilian dry‐cured loin: a chemometric approach
Authors: Mutz Yhan S. , Kaic Alves Rosario Denes , Alves de Aguiar Bernardo Yago , Paulo Vieira Carla , Vilela Pinto Moreira Rodrigo , Bernardes Patrícia C. , Conte‐Junior Carlos A. 
Published: December 2021

AbstractExploratory data analysis was used to evaluate the influence of NaCl concentration on the relation between endogenous microbiota (mesophilic aerobic bacteria, psychrotrophic bacteria, lactic acid bacteria, Enterobacteriaceae and Staphylococcus spp.) and biogenic amines (BAs) (histamine, tyramine, putrescine, cadaverine and spermidine) content in Brazilian dry‐cured loin (BDL). Using hierarchical cluster analysis (HCA), initial data analysis led to samples separation into a higher and a lower NaCl cluster. The analysis of variance showed that the microbial counts did not differ between the clusters (p > 0.05). However, the higher NaCl cluster showed a lower level of BAs. Further, principal component analysis (PCA) demonstrated a negative correlation between the microbial counts and BAs content in the lower NaCl cluster, thus indicating the possibility of the higher BA’s content in the lower NaCl cluster being a result of a stress–response mechanism. On the other hand, in the higher NaCl cluster, the salt concentration had an inhibitory property in BA’s formation except for histamine. The collective results point to a NaCl threshold to minimise the production of BAs in BDL. The findings of the present exploratory study highlight the possibility of intervention for BA minimization without the need for designed starter cultures or preservation treatments.
Effect of pH on the coagulation properties of Tenebrio molitor coagulant
Authors: Song Zhaoyang , Zhang Yan , Wen Pengcheng , Wang Yue , Qiao Haijun , Zhang Weibing , Zhang Zhongming 
Published: December 2021

SummaryBecause of the continuous increase in global cheese consumption, there is a continual need to find new coagulants for producing cheeses with different characteristics to suit various consumers. A new coagulant was extracted from Tenebrio molitor larvae. We then investigated the influence of pH on the coagulation properties of Tenebrio molitor coagulant. The results indicate that the rennet flocculation time and whey OD value of Tenebrio molitor coagulant increased with the increase in coagulation pH (P < 0.05). The water holding capacity, turbidity, particle size and storage modulus increased and then decreased (P < 0.05) and reached the maximum at pH 5.6. There was a decrease and then increase for casein glycopeptide in the hydrolysis solution (P < 0.05), and its content reached 0.64 mg/mL at pH 6.2; the minimum hydrolysis of Tenebrio molitor coagulant was found at pH 5.6. SDS‐PAGE, secondary structure and confocal laser scanning microscope indicated that the curd structure was stable at pH 5.6, and the coagulation performance was better. Moreover, there were the smallest differences for the texture structure of Tenebrio molitor coagulant curd with commercial rennet curd at pH 5.6. Therefore, pH 5.6 is the optimum pH of Tenebrio molitor coagulant. The study provides a theoretical basis and reference for the development of a new edible insect coagulant and its application.
A comparative study on the physicochemical, cooking and textural properties of fortified rice kernels prepared from raw and parboiled rice
Authors: Nithya A. , Dalbhagat Chandrakant Genu , Mishra Hari Niwas 
Published: December 2021

SummaryFortified rice kernels (FRK) are extruded rice‐shaped kernels manufactured by blending broken rice flour and vitamin–mineral premix. In this study, four parboiled and three raw rice with intermediate amylose content (20–24%) were processed to manufacture FRK. The system parameters (torque and die pressure), physicochemical properties (length, lightness, water absorption index and water solubility index), cooking properties (cooking time, solid loss, elongation) and textural properties of FRK were analysed and correlated using the Pearson correlation coefficient. The torque and die pressure were 16.76–22.76 Nm and 92.56–136.41 bar, respectively. Compared to raw rice, the parboiled rice showed the highest torque, die pressure and axial expansion in uncooked FRK while longer cooking times, higher elongation ratio and hardness; lesser water absorption ratio and solid loss in cooked FRK. The raw rice FRK showed higher lightness. There was a significant difference between all FRK for water absorption index (1.74–2.52) and water solubility index (2.06–3.53%).
Presence of perfluoroalkyl substances in Mediterranean sea and North Italian lake fish addressed to Italian consumer
Authors: Chiesa Luca Maria , Pavlovic Radmila , Arioli Francesco , Nobile Maria , Di Cesare Federica , Mosconi Giacomo , Falletta Ermelinda , Malandra Renato , Panseri Sara 
Published: December 2021

SummaryThe primary source of perfluoroalkyl substances (PFASs) exposure is the diet, mainly fish. Seabass (Dicentrarchus labrax) and seabream (Sparus aurata) are among the most consumed sea fish in Italy. Fish from Northern Italy lakes like perch (Perca fluviatilis) is of particular importance in terms of consumption volume, while Mediterranean shad (Alosa agone) and European whitefish (Coregonus lavaretus) represent niche consumption. These species too could be a cause of consumer exposure to PFAS. The study investigated the presence of PFASs in the above‐mentioned fish species through LC‐HRMS analysis. Only PFBA and PFOS were found both in lake and sea fish while PFBS was found only in lake fish. The found concentrations were used to estimate intake of PFOS, the only detected molecule of the four discussed in the last EFSA report. The Tolerable Weekly Intake of 4.4 ng kg−1 b.w. per week was never exceeded also for the 99th percentile consumers.
Role of Vesical Imaging–Reporting and Data System in predicting muscle‐invasive bladder cancer: A diagnostic meta‐analysis
Authors: Yuan Baorui , Cai Lingkai , Cao Qiang , Wu Qikai , Zhuang Juntao , Sun Xueying , Zhang Yudong , Li Pengchao , Yang Xiao , Lu Qiang 
Published: December 2021

AbstractThe objective of this study is to systematically evaluate the diagnostic performance of the Vesical Imaging–Reporting and Data System for predicting muscle‐invasive bladder cancer. Embase, PubMed and Web of Science were systematically searched from 1 September 2018 to 30 July 2021 to include proper studies. We included studies that included data on Vesical Imaging–Reporting and Data System and their associated pathological findings, and we assessed their quality using the Quality Assessment of Diagnostic Accuracy Studies‐2 tool. The pooled sensitivity and specificity were calculated and plotted using hierarchical summary receiver operating characterisijutic modeling. Meta‐regression analysis was carried out to detect heterogeneity. A total of 20 studies with 2725 patients were included. When the cut‐off point was 3, the pooled sensitivity and specificity were 0.92 (0.89–0.94) and 0.85 (0.78–0.90), respectively, and 0.82 (0.75–0.88) and 0.95 (0.91–0.97), respectively, when the cut‐off point was 4. The area under the curve was 0.95 and 0.95, respectively. Heterogeneity was substantially considerable in sensitivity and specificity. All subgroup variables, including patient number, study design, magnetic resonance imaging field strength, number of radiologists, surgery pattern, diffusion‐weighted imaging, and dynamic contrast‐enhanced magnetic resonance imaging, contributed to sensitivity heterogeneity when the cut‐off point was 3 and specificity heterogeneity when the cut‐off point was 4. Multiple image acquisition plane of diffusion‐weighted imaging achieved a higher sensitivity than single image acquisition plane of diffusion‐weighted imaging in both the Vesical Imaging–Reporting and Data System 3 and 4 groups, and higher specificity in the Vesical Imaging–Reporting and Data System 4 group. Another significant source of heterogeneity was the cut‐off point. The diagnostic performance of the Vesical Imaging–Reporting and Data System for predicting muscle‐invasive bladder cancer was excellent in both cut‐off points of the Vesical Imaging–Reporting and Data System 3 and 4. Multiple image acquisition planes of diffusion‐weighted imaging should be given more attention in the Vesical Imaging–Reporting and Data System.
Insights into the development of a new index, vesical adaptation response to diuresis, for understanding lower urinary tract dysfunction
Authors: Matsuoka Kanako , Akaihta Hidenori , Hata Junya , Imai Hitomi , Tanji Ryo , Honda‐Takinami Ruriko , Hoshi Seiji , Koguchi Tomoyuki , Sato Yuichi , Kataoka Masao , Ogawa Soichiro , Kojima Yoshiyuki 
Published: December 2021

ObjectivesTo clarify how vesical adaptation response, the homeostatic system that constantly changes voided volume to adapt to diuresis, is involved in male lower urinary tract symptoms and bladder storage function.MethodsWe included male patients older than 65 years with lower urinary tract symptoms. Vesical adaptation response to diuresis was defined as a positive correlation between urine output rate and voided volume on 3‐day sensory‐related frequency volume charts. Patients were divided into two groups according to the presence or absence of vesical adaptation response to diuresis, and characteristics were compared between groups.ResultsNinety‐four male patients were finally analyzed. Vesical adaptation response to diuresis was found in 48 patients (51%) and was lacking in 46 patients (49%). Patients without vesical adaptation response to diuresis were significantly more often diagnosed with overactive bladder (P = 0.04). After adjusting for confounders, absence of vesical adaptation response to diuresis was significantly associated with overactive bladder (adjusted odds ratio 3.76, 95% confidence interval 1.34–10.55; P = 0.01) and benign prostatic enlargement (adjusted odds ratio 1.04, 95% confidence interval 1.01–1.07; P = 0.02).ConclusionsThe absence of vesical adaptation response to diuresis, characterized by decreased voided volume during a diuretic phase, can be interpreted as a form of bladder storage dysfunction. Assessment of vesical adaptation response to diuresis may provide a new index of bladder storage function and contribute to a better understanding of the pathophysiology underlying bladder storage dysfunction in patients with lower urinary tract symptoms.
Regulatory function of the trehalose‐6‐phosphate synthase gene TPS3 on chitin metabolism in brown planthopper, Nilaparvata lugens
Authors: Zhou Min , Shen Qida , Wang Shasha , Li Guoyong , Wu Yan , Xu Caidi , Tang Bin , Li Can 
Published: December 2021

AbstractBrown planthopper (Nilaparvata lugens) is one of the important pests that damage rice. Trehalose‐6‐phosphate synthase (TPS) is a key enzyme responsible for catalysing the biosynthesis of trehalose, which is the energy substance of insects. In this study, combined with the reported N. lugens TPS1, TPS2 and newly discovered TPS3, we studied the regulation of TPS in chitin metabolism by RNA interference. Firstly, we found that the relative expression levels of TRE1‐1, TRE1‐2 and TRE2 increased significantly after 48 h of dsTPS3 injection, and the activity of TRE1 enhanced significantly. Secondly, abnormal and lethal phenotypes were observed after dsTPS3 and dsTPSs injection. The relative expression levels of PGM2, G6PI2, Cht1‐4, Cht6‐10 and IDGF decreased significantly after 48 h of dsTPS3 injection. At 72 h after injection of dsTPS3, the relative expression levels of CHS1, Cht2, Cht4, Cht7 and Cht8 reduced significantly, but the expression levels of G6PI1, Cht5 and ENGase increased significantly. The relative expression levels of GFAT, UAP, PGM2, G6PI2, CHS1, CHS1a, CHS1b, Cht2, Cht4, Cht8, Cht9 and Cht10 decreased significantly after 48 h of dsTPSs injection. However, at 72 h after the injection of dsTPSs, the expression levels of GNPNA, UAP, PGM1, G6PI1, HK, CHS1, CHS1a, CHS1b, Cht3, Cht5, Cht7 and ENGase increased significantly. Finally, the chitin content decreased in dsTPS1, dsTPS2 and dsTPSs treatments. In conclusion, the inhibition of TPS expression affected the metabolism of trehalose and chitin in N. lugens. The related research results provide a theoretical basis for pest control.
Biochemical characterization and overexpression of an α‐amylase (BmAmy) in silkworm, Bombyx mori
Authors: Yan Hao , Wen Feng , Xiang Haiying , Wen Yuchan , Shang Deli , Liu Anyang , Niu Yicheng , Xia Qingyou , Wang Genhong 
Published: December 2021

AbstractSilkworm (Bombyx mori) is the only fully domesticated insect. As an economically important insect, nutrition utilization is important for its productivity. Hence, the present study investigated the expression pattern of BmAmy, an α‐amylase, in B. mori. BmAmy protein purification and biochemical characterization were performed, and effects of BmAmy overexpression were assessed. Real‐time quantitative reverse transcription polymerase chain reaction indicated that BmAmy transcription was positively correlated with the silkworm's food intate. Moreover, enzymatic activity assay results showed that BmAmy had significant α‐amylase activity of about 1 mg/min/mg protein. Furthermore, treatment with mulberry amylase inhibitors MnAI1 and MnAI2 resulted to 89.92% and 93.67% inhibition in BmAmy activity, respectively, and the interaction between BmAmy and MnAI was also confirmed by protein docking analysis. A silkworm line that specifically overexpressed BmAmy in the midgut was generated through piggyBac‐based transgenic technology, and compared to those of non‐transgenic silkworms, the whole cocoon and cocoon shell weights of these transgenic silkworms increased by 10.13% and 18.32%, respectively, in the female group, and by 5.83% and 6.00%, respectively, in the male group. These results suggested that BmAmy may be a suitable target for breeding better silkworm varieties in the future.
Transcriptome analysis and identification of sex pheromone biosynthesis and transport related genes in Atrijuglans hetaohei (Lepidoptera: Gelechioidea)
Authors: Zhu Shuying , Shaheen Tayyab , Li Rong , Pei Pei , Zhao Xing , Li Yan , Li Feifei , Tang Guanghui 
Published: December 2021

AbstractAtrijuglans hetaohei Yang (Lepidoptera: Gelechioidea) is one of the major pests that can seriously damage the walnut tree, leading to harvest loss. Sex pheromones regulate mating communication and reproduction in insects and provide targets for developing a novel pest control strategy. In this study, by transcriptomic sequencing and analysis of the female pheromone gland (PG) and male genitalia of A. hetaohei, we identified 92 putative genes, of which 7 desaturases (Dess), 8 fatty acyl reductases (FARs), 4 fatty acid synthetases (FASs), 2 aldehyde oxidases (AOXs), 4 acetyltransferases (ACTs), 1 chemosensory protein (CSP), and 2 odorant‐binding proteins (OBPs) were predominantly expressed in the female PG, while 5 Dess, 11 FARs, 7 FASs, 6 AOXs, 1 ACT, and 1 CSP showed more robust expression in the male genitalia. Moreover, phylogenetic analysis revealed that 7 Dess and 1 FAR were grouped with genes involved in pheromone synthesis in other Lepidoptera species. Thus, we proposed that these candidate genes are possibly involved in the sex pheromone biosynthetic pathway in A. hetaohei. Our findings will provide a solid genetic basis for further exploring the function of the tissue‐biased genes and may be useful to screen potential targets for interfering chemical communication in A. hetaohei.