bluuebunny/crossref_metadata_2025_split huggingface dataset:
A comparison of the efficacy and safety of complementary and alternative therapies for polycystic ovary syndrome A protocol for network meta-analysis
Authors: Pan Jing , Xiang Shan , Dong Shuangqian , Qin Xiuyun , Zhang Jianwei 
Published: February 2021

Abstract
Background: With the increase in the incidence of Polycystic Ovary Syndrome (PCOS) over the years, restoring ovulation function has become one of the important contents of reproductive medicine research today. PCOS affects reproductive ability and harms physical and mental health. Studies show both complementary and alternative therapies to be effective in treating PCOS. However, the consistency of the conclusion is still inadequated. In view of this, we will develop a research to evaluate the effectiveness and safety of complementary and alternative therapies for PCOS. We therefore carry on a study protocol for a proposed network meta-analysis (NMA) and systematic review on PCOS.Methods: The following electronic bibliographic database will be searched: PubMed, Chinese National Knowledge Infrastructure (CNKI), VIP database, Wanfang database, The Cochrane Library, EMBASE and Web of Science from inception till 31 December2020. A search at the World Health Organization (WHO) International Clinical Trials Registry Platform will also be done. Two researchers will independently screen literature, extract data and assess the risk of bias of included studies. Analysis will be performed on included studies using the NMA technique. Data will be analyzed using ADDIS 1.16.8 and STATA 15.0.Results: This work will evaluate the safety and effectiveness of alternative and complementary therapies used in the treatment of PCOS.Conclusion: The results of this study will provide reliable evidence for clinicians in the field to build on for best practices in effective interventions (complementary and alternative therapies) for PCOS.
Optimizing Quality of Electronic Medical Records as a Platform for Resident Education
Authors: Hung Hsuan , Kueh Ling-Ling , Tseng Chin-Chung , Huang Han-Wei , Wang Shu-Yen , Hu Yu-Ning , Lin Pao-Yen , Wang Jiun-Ling , Chen Po-Fan , Liu Ching-Chuan , Roan Jun-Neng 
Published: February 2021

Abstract
Background Electronic medical records (EMRs) are becoming a predominant feature of health care and an important tool in residents’ medical education. Few studies have examined and compared the EMR performance of residents from different departments. This study explores the quality of EMRs in residency and offers pedagogical implications.Method Authors developed an EMR checklist which examined fundamental EMR requirement through six dimensions and 20 items. A total of 149 records created by residents from 32 departments/stations were randomly chosen. Seven senior physicians rated the EMRs using the checklist. Medical records were grouped as General Medicine, Surgery, Pediatric, Obstetrics and Gynecology and Other Departments. Overall and grouped performances were analyzed by ANOVA.Results Overall performance was rated as fair to good. Regarding the six dimensions, the discharge note (0.81) gained the highest scores, followed by the admission note (0.79), problem list (0.73), overall performance (0.73), progress note (0.71), and weekly summary (0.66).Among the five groups, Other Departments (80.20) gained the highest total score, followed by Obstetrics and Gynecology (78.02), Pediatrics (77.47), General Medicine (75.58), and Surgery (73.92).Conclusions The quality of EMRs, core competencies, and clinical reasoning skills are deeply interconnected in resident education. This study suggested that the use of duplication in EMR, and the communication abilities of residents are associated with the variable quality of medical records in different departments. Further study is required to apply the insights obtained in this study to improve the quality of EMRs, and thereby the effectiveness of resident training.
miR-106b regulates the reprogramming of spermatogonial stem cells into iPSC-like cells
Authors: Fard Amir Hossein Hasani , Valizadeh Mahmoud , Mazaheri Zohreh , Hosseini Jalil 
Published: February 2021

Abstract
Background

Recent years have brought notable progress in raising the efficiency of the reprogramming technique, so that approaches have evolved from known transgenic factors to only a few microRNAs. Nevertheless, there is a poor understanding of both the key factors and biological networks underlying this reprogramming. Therefore, the present study aimed to investigate the potential of miR-106b in regulating Spermatogonial stem cells (SSCs) to iPSC-like cells. We used SSCs because pluripotency can be induced in them under defined culture conditions with fewer issues compared to other adult stem cells.

Methods

As both signaling and post-transcriptional gene control are critical for the regulation of pluripotency, we traced the expression of Oct-4, Sox-2, Klf-4, c-Myc, and Nanog (OSKMN), and studied miR-106b targets using bioinformatic methods.

Results

Our results showed that transfected SSCs with miR-106b increased expression of the OSKMN factors, and this expression in iPSC and induced SSC groups was significantly more than negative control groups. Moreover, using the functional miRNA enrichment analysis, online tools, and databases we predicted that miR-106b targeted a signaling pathway gene named MAPK1/ERK2, which regulates stem cell pluripotency.

Conclusions

Together, these data suggest that miR-106b regulates reprogramming of SSCs into iPSC-like cells by targeting the ERK2 gene as a part of the regulatory network that controls the pluripotency state and reprogramming process.
Cardioprotection of Sodium–Glucose Cotransporter 2 Inhibition in Rats With Isoproterenol-Induced Cardiomyopathy
Authors: Wang Fang-Zheng , Wei Wen-Bo , Li Xin , Huo Jun-Yu , Jiang Wan-Ying , Wang Hong-Yu , Qian Pei , Li Zhen-Zhen , Zhou Ye-Bo 
Published: February 2021

Abstract
Background: Sodium-glucose cotransporter 2 inhibitor (SGLT2i) has been reported to improve glycaemic control in patients with type 2 diabetes. The aim of this study was to investigate the effect of SGLT2i Dapagliflozin (Dapa) on cardiomyopathy induced by isoproterenol (ISO) and its potential mechanism.Methods: Fifty male Sprague Dawley rats were randomly assigned to Control (n = 10) and ISO (2.5 mg/kg/day)-treated groups (n = 40). After 2 weeks, 28 survived rats with obvious left ventricular dysfunction in ISO group were randomized into three groups for medication including ARNI (angiotensin receptor neprilysin inhibitor, 68 mg/kg/day, n = 9), Dapa (3 mg/kg/day, n = 9) and ISO (saline, n = 10) for 4 weeks. After that, electrical programmed stimulation (EPS) was performed in all groups for the evaluation of the susceptibility of ventricular arrhythmias (VAs). Echocardiography was used to evaluate cardiac function. Results: Echocardiography revealed significant left ventricular (LV) dysfunction in rats with ISO treatment for 2 weeks compared to the control group. Dapa administration for 4 weeks reduced the cumulative risk of death, myocardial fibrosis, plasma angiotensin II level and its functional receptor AT1R protein expression in the heart, and proinflammatory cytokines levels in the cardiac tissue of ISO-treated rats. It also improved cardiac function and inhibited oxidative stress when compared to the ISO group. These effects were similar to ARNI. However, Dapa showed a greater efficacy than ARNI in reducing left ventricular end-diastolic volume, lowing heart rate and VAs, and decreasing body weight and plasma glucose in ISO-treated rats. Conclusion: Dapa effectively improved the myocardial remodelling and oxidative stress like ARNI in ISO-induced cardiomyopathy in rats, but Dapa may be more effectively in decreasing VAs, and improving cardiac function when compared to ARNI. The mechanisms by which Dapa exerts protective effects on cardiomyopathy may be related to its antioxidant capacity and hypoglycemic action.
Knockdown of SIPA1L3 Inhibits the Proliferation and Invasion of Non–Small Cell Lung Cancer Cells Through the Hippo Pathway
Authors: Wang Si , Han Qiang , Rong Xue-Zhu , Sun Ming-Fang , Diao Ke-Xin , Wang En-Hua , Liu Yang 
Published: February 2021

Abstract
Background: SIPA1L3 is a member of the signal-induced proliferation-associated (SIPA) protein family, only limited data about the SIPA1L3 are currently available in human carcinoma. Our present study provides the expression pattern and function of SIPA1L3 in non-small cell lung cancer (NSCLC).Methods: We performed immunohistochemistry to detect the distribution of SIPA1L3 in NSCLC specimens and analyzed the relationship between SIPA1L3 expression and patients clinicopathological feature. We used small interfering RNA (siRNA) to specifically silence SIPA1L3, then investigated its effect on cell growth and invasion in human lung cancer cell lines. Western blot and immunoprecipitation were performed to show the interaction of SIPA1L3 with the core proteins of Hippo pathway, and the Hippo pathway activity.Results: Immunohistochemical staining showed that SIPA1L3 was cytoplasmic increasing in 147 of 217 cases. High levels of SIPA1L3 expression were associated with poor differentiation and a high tumor node metastasis stage, positive lymph nodal status, and poor prognosis. Downregulation of SIPA1L3 inhibited cell EMT, growth, and invasion. As well as SIPA1L3 inhibited Hippo pathway. SIPA1L3 interacted with AMOT, which inhibited AMOT binding to Pals, then decreased nuclear location of YAP.Conclusions: SIPA1L3 overexpression may be a marker for advanced NSCLC. SIPA1L3 reduction inhibits the proliferative and invasive ability of the lung cancer cells, which involves the Hippo pathway by contacting with AMOT.
Information Retrieval Sources and Skill, and Knowledge of Evidence-based Dentistry Among Iranian Dental Students
Authors: Sistani Mohammad Mehdi Naghibi , Kazemian Ali , Virtanen Jorma I 
Published: February 2021

Abstract
Background: This study aimed to determine the common scientific information sources used, information retrieval skills, and knowledge of evidence-based dentistry (EBD) among graduating dental students in two dental schools in Iran.Methods: In total, 179 final-year dental students from Mashhad University (71%) and Babol University (29%) participated in this anonymous survey in 2019. A valid self-administered questionnaire has been translated using forward-backward method to assess students' use of information sources for the past six months, their information retrieval skills, and knowledge of EBD. We used chi-square test, t-test, and one way ANOVA for statistical analysis.Results: Most commonly used information sources by the students in the university and at home were: personal lecture notes, fellow dental students, and Compact Dentistry References (CDR) books. Proportion of students with good knowledge of EBD was 53.6%. Dental students who sensed to possess weaker information retrieval skills revealed lower EBD scores (p=0.01). Students who used at least one evidence-based information source (Cochrane, PubMed/Medline, scientific journals’ websites) more frequently sensed to possess better information retrieval skills (p=0.02).Conclusions: Commonly used information sources by the dental students were not evidence-based. Those students with better information retrieval skill showed higher EBD knowledge score and used evidence-based information sources more frequently. Therefore, more efforts should be put to basic skill of information retrieval and EBD in Iranian dental curricula.
WITHDRAWN: Combining photo-elicitation and discourse analysis to examine adolescents’ sexuality in rural Zambia
Authors: Mulubwa Chama , Hurtig Anna-Karin , Zulu Joseph Mumba , Michelo Charles , Sandøy Ingvild Fossgard , Goicolea Isabel 
Published: February 2021

Abstract
Introduction: This article aimed to analyse constructions of adolescents’ sexualities and sexual health and the consequences of these discourses for adolescents’ exercise of their sexual reproductive health and rights (SRHR) in rural Zambia. Methods: We analysed data from photo-elicitations interviews and focus group discussions with 25 adolescents who participated in a SRHR intervention that aimed to reduce adolescents’ pregnancies and early marriages. Results: We identified three interpretative repertories: 1) sex is for mature people in which adolescents positioned themselves as ‘immature, and young to engage in sex; 2) gendered respectful behaviours in which what was considered disrespectful (and respectful) behaviour in relation to sexuality were strongly influenced by gender, and more clearly defined for girls than it was for boys. Sexuality was not only about individual choices but about being respectful to parents; and 3) acquiring and using knowledge about sexuality in which adolescents conflicted between having and applying SRHR knowledge. Conclusion: These repertories offer an important context that shape how adolescents negotiate, adopt and resist SRHR interventions. Future interventions that target adolescents’ SRHR must aim to address the sexual scripts that have serve to erect barriers against positive sexual behaviours, including access to SRHR services that promote safer sex.
Potential Target Screening and Molecular Docking of Newly Isolated Compound From Inonotus Obliquus
Authors: Liu Zhen-Dong , Liu Yi , Li Liang , Xue Bei , Wang Wenhan , Yan Xiu-Feng 
Published: February 2021

Abstract
Compound (1-(4-hydroxyphenyl)-3-methoxypropan-1-one) is a newly isolated compound from Inonotus obliquus. In this paper, four target protein prediction methods were used to search the potential targets of compounds. The target proteins were determined according to the corresponding screening criteria. The target proteins with therapeutic value were docked with compound and target proproteinogen ligands by molecular docking software, and their binding affinity with target proteins was compared. The pharmacophore analysis program was used to investigate whether the pharmacophore characteristics of the compound and the original ligand compounds were consistent. 26 target proteins were obtained by virtual screening. The PASS server filtered out 2 targets. 7 targets were screened by the PharmMapper database. 2 targets were obtained by screening the PDB protein database. 18 targets were obtained by ROCS screening. Compared with the binding affinity of molecular docking, it was found that the binding affinity of the compound to oxysterols receptor LXR-beta, chymase, heat shock protein HSP 90-alpha, carbonic anhydrase 2 and arylamine N-acetyltransferase Nat was higher than that of the original ligands. It shows that the compound has potential applications in anti-tumor, anti-inflammatory, affecting intestinal flora and affecting human lipid transport. Pharmacophore analysis was performed using LigandScout software. The results of pharmacophore analysis show that the phenolic hydroxyl group in the compound is the key group and can participate in the interaction with amino acids. Besides, the mother nuclear benzene ring can also participate in the hydrophobic interaction.
Clinical and Immune Responses of Peripheral Chemical Sympathectomy in Enterovirus 71 Infection&nbsp;
Authors: Liao Yu-Ting , Tsai Huey-Pin , Wang Shih-Min , Chen Shun-Hua 
Published: February 2021

Abstract
Background: The activation of the sympathetic nervous system, release of norepinephrine (NE), and adrenergic receptor signaling participate in and regulate the complicated enterovirus 71 (EV71) brainstem encephalitis (BE). The neurotoxin 6-hydroxydopamine (6-OHDA) selectively ablates sympathetic nerves and markedly depletes NE in innervated organs.Methods: A mouse-adapted-EV71 strain (MP4) model was used to determine the effects of 6-OHDA on the survival rate, clinical score, plasma levels of norepinephrine (NE), epinephrine (EP), dopamine (DP), and cytokines. The peripheral blood mononuclear cells and splenocytes of mouse were analyzed by flow cytometry.Results: The survival rate (76.9%) of MP4-infected after 6-OHDA-treated mice was increased significantly. The clinical scores were decreased markedly on days 8-12 in MP4-infected after 6-OHDA-treated mice compared to those without treatment. The plasma levels of NE, EP, and DP were decreased at most on day 8 after 6-OHDA treatment. The plasma levels of interleukin (IL)-12p70, tumor necrosis factor, IL-6, and IL-10 did not change significantly after 6-OHDA treatment. Interferon-g levels decreased evidently on days 4, 6, and 8 after 6-OHDA treatment. The absolute events of CD3+CD4+, CD3+CD8+, and CD3+NK1.1+ cells of peripheral blood mononuclear cells and the absolute cells of CD3−NK1.1+ and CD3+NK1.1+ cells in splenocytes were increased significantly in MP4-infected after 6-OHDA-treated mice compared to those without treatment. Conclusions: These findings suggested that 6-OHDA may be used a probe to explore clinical improvements and immune responses in the complicated EV71 infection. These properties together contribute to the immunopathogenesis of EV71 BE with autonomic nervous system dysregulation.
Effects of TiO2 nanoparticle lubricant on micro deep drawing performance
Authors: Pan Di , Zhang Guangqing , Jia Fanghui , Kamali Hamidreza , Lu Yao , Lin Fei , Jiang Zhengyi 
Published: February 2021

Abstract
Micro deep drawing is a process to manufacture the thin walled, hollow, box or cup like products at micro scale. Lubricant can affect the products’ quality in micro deep drawing at micro scale due to the decrease of coefficient of friction between the material and tools, it is crucial to enhance the forming efficiency. In this study, 40 µm thickness stainless steel 301 (SUS301) was annealed at 980 ℃ for 2 min under protection of argon gas ambient, and this stainless steel was selected as the specimen material. The micro deep drawing was conducted on a micro press machine under dry and 4% TiO2 nanoparticle lubrication conditions with different forming velocities. The experimental results showed that the micro cup’s profile is affected by changing the forming velocity under the dry and nanoparticle lubrication conditions. Under the dry condition, the surface became rough with the increase of the forming velocity, and then the micro forming efficiency under application of nanoparticle lubricant increases with a rise of drawing velocity.
An evaluation of three-dimensional facial changes after surgically assisted rapid maxillary expansion (SARME)
Authors: Zupan Jurij , Hren Nataša Ihan , Verdenik Miha 
Published: February 2021

Abstract
Background: The purpose of the study was to evaluate the three-dimensional (3D) facial changes following surgically assisted rapid maxillary expansion (SARME). In 15 skeletally mature patients with maxillary transverse deficiency the planned maxillary expansion (on average 8.8 mm ± 2.3 mm) was achieved with bone-borne palatal distractor. The 3D optical scans of the facial surface were obtained before and 6 months after SARME and compared with cephalometric and regional analysis to evaluate the soft-tissue changes. Findings. The largest differences between the pre- and post-operation scans were observed for the paranasal and cheek area (1.4 ± 1.0 mm) in the lateral direction with a soft-to-hard tissue ratio of 0.22. Significant differences occurred for an increased nasal width, a decreased upper-face height with an unchanged lower height, an increased vertical philtrum height and an increased nasolabial angle. A significant increase in the facial profile angle was also observed, resulting in an increased facial convexity and anterior displacement of the upper-lip area. Conclusions. We have confirmed the widening of the nose and increased projection in the cheek and paranasal area in the lateral direction after maxillary expansion, while we found facial convexity increases, reflecting the underlying advancement of the maxilla.
Assessing the impact of working pressure on water meter registration
Authors: Musaazi Isaac G. , Sempewo Jotham I. , Babu Mohammed , Kiggundu Nicholas 
Published: February 2021

Abstract
Fluctuations in the network pressure of water supply systems affect hydraulic performance and water meter accuracy. The development of metering error curves requires steady-state conditions which are extremely rare in water distribution systems characterized by intermittent supply. Simple deterministic models are suggested and developed from monthly data collected over a 4-year period (2010–2014) for three most dominant meter models (Models 1–3) in the Kampala Water Distribution System (KWDS), Uganda. This study combines pressure and billing information at the same time to understand metering accuracy. Results showed that metering accuracy increased by 4.2% for Model 1, 8.4% for Model 2 and 2.9% for Model 3, when the pressure was increased from 10 to 50 meters head. Age did not influence the impact of pressure on meter accuracy. The most sensitive parameter in the model was the meter age. Metering accuracy was relatively constant after a period of 5 years. The least sensitive parameter was the working pressure which caused a slight change to the annual billed volume. The ability of the model to accurately predict the meter registration degenerated with an increasing annual billed volume. Model 2 meters were the best performing and probably the most suitable meters in the KWDS.
Elucidating the Drug Repurposing Spectra of COVID-19 with its
Analogues SARS and MERS
Authors: Mishra Jayanti , Prasun Chakrawarti , Sahoo Pravat K. , Nair Maya S. 
Published: February 2021

Abstract:
Corona Virus Disease-2019 (COVID-19), caused by the SARS CoV-2 virus, has been announced
as a pandemic by the World Health Organization. COVID-19 has affected people globally,
infecting more than 39.8 million people and claiming up to 1.11 million lives, yet there is no effective
treatment strategy to cure this disease. As vaccine development is a time-consuming process, currently,
efforts are being made to develop alternative plans for the timely and effective management of this
disease. Drug repurposing always fascinated researchers and can be utilized as the most acceptable
alternative to develop the therapeutics for COVID-19 using the pre-approved drugs. Severe acute respiratory
syndrome coronavirus 2 (SARS-CoV-2) has shown resemblance with distinctive enzyme targets,
such as 3CLpro/Mpro, RdRp, Cathepsin L, and TMPRSS2 present in SARS CoV and MERS
CoV. Therefore, the drugs that have shown efficacy in these viruses can also be used for the treatment
of COVID-19. This review focuses on why repurposing could provide a better alternative in COVID-
19 treatment. The similarity in the structure and progression of infection of SARS CoV and MERS
viruses offers a direction and validation to evaluate the drugs approved for SARS and MERS against
COVID-19. It has been indicated that multiple therapeutic options that demonstrate efficacy against
SARS CoV 2 are available to mitigate the potential emergence of COVID-19 infection.