bluuebunny/crossref_metadata_2025_split huggingface dataset:
Gonadal tumor and malignancy in 118 patients with disorders of sex development with Y chromosome
Authors: Ouyang Yunwei , Tan Shiqiao , Yu Ya , Luo Bing , Yin Weiyao , Luo Li 
Published: October 2021

AbstractObjectiveTo provide more information about tumor prevalence and malignant transformation among patients with disorders of sex development (DSD) for further guidance in prophylactic gonadectomies and surveillance.MethodsSPSS software (version 20.0) was used for all statistical analyses.ResultsPhenotypically female DSD patients with a Y chromosome have a higher risk of gonadal malignancy.ConclusionBilateral gonadal resection is recommended as soon as diagnosis is made for phenotypically female patients with disorders of sex development with a Y chromosome.
Comparison of nitrogen assimilation components among Agrostis species and cultivars
Authors: DaCosta Michelle , Ebdon Jeffrey Scott , Chappell Lindsey Hoffman 
Published: October 2021

AbstractEnhanced nitrogen use efficiency (NUE) in turfgrass systems comprises several factors, including more efficient uptake and assimilation of applied nitrogen (N), as well as reduced N losses to the environment. The objectives of the current research were to evaluate the inter‐ and intraspecific variation in N use parameters in two Agrostis species, creeping bentgrass (Agrostis stolonifera L.) and velvet bentgrass (Agrostis canina L.), under field and controlled environmental conditions. Measurements included leaf NUE (g dry matter mg–1 N), leaf N content (mg g–1 dry matter), leaf and root nitrate reductase activity (NRA)(μmol NO2– g–1 fresh weight h–1), and root nitrate uptake rate (NUR). Velvet bentgrass cultivars exhibited higher leaf N content and therefore lower leaf NUE than creeping bentgrass cultivars in the field and in a nutrient solution. Differences in leaf NRA were only observed in Agrostis species and cultivars in the field, with higher NRA observed in creeping bentgrass. Velvet bentgrass cultivars grown in a nutrient solution exhibited a 50% higher root NUR than creeping bentgrass, with leaf NRA and N content increasing and leaf NUE decreasing in Agrostis under higher NO3– supply.
Research and scholarly methods: Semi‐structured interviews
Authors: Adeoye‐Olatunde Omolola A. , Olenik Nicole L. 
Published: October 2021

AbstractThe popularity and value of qualitative research has increasingly been recognized in health and pharmacy services research. Although there is certainly an appropriate place in qualitative research for other data collection methods, a primary benefit of the semi‐structured interview is that it permits interviews to be focused while still giving the investigator the autonomy to explore pertinent ideas that may come up in the course of the interview, which can further enhance understanding of the pharmacy service being assessed. The purpose of this narrative review is to summarize methodological considerations and procedures for conducting semi‐structured interviews in pharmacy services research. In this article, we propose the Seven Steps to Conducting, Analyzing, and Reporting Semi‐Structured Interview Data (7S CARS‐SID) for Pharmacy Services Research. While many of the proposed steps can be applied to various qualitative methods and types of research, this narrative review intentionally focuses discussion on semi‐structured interviews and pharmacy services research. These seven steps along with the cited resources and applicable examples provide novice qualitative researchers with a step‐by‐step introductory guide to conducting qualitative pharmacy services research using semi‐structured interview methods. Finally, the 7S CARS‐SID for Pharmacy Services Research is intended to be a tool for assisting readers, reviewers, and editors of the Journal of the American College of Clinical Pharmacy to better understand the methodology behind qualitative research papers using semi‐structured interview methods.
Strategies for the integration of foundational and clinical sciences in doctor of pharmacy programs
Authors: Malhotra Ashim , Reddy Indra K. , Fulford Michael , Khasawneh Fadi T. , Tiwari Amit K. , Feng Xiaodong 
Published: October 2021

AbstractIntroductionEnhancing the integration of foundational and pharmaceutical sciences with the clinical pharmacy curriculum will develop practitioner‐scholar pharmacists. However, implementing and assessing integration is challenging. Hindrance factors include limited pragmatic approaches, the lack of a “how‐to” blueprint, faculty and student resistance to change, compression of pharmaceutical science teaching, and a paucity of assessment strategies.MethodsThis study examined the history, theory, and practice of integration and utilization of the 2013 Center for Advancement of Pharmacy Education (CAPE) Outcomes to achieve integration in Doctor of Pharmacy (PharmD) programs. For this, 252 faculty members across the American Association of Colleges of Pharmacy (AACP) Biological Sciences, Pharmaceutics, and Chemistry sections were surveyed to assess faculty perception regarding (1) the importance of integrating foundational and clinical sciences, (2) the extent, and (3) intentionality of incorporation of the 2013 CAPE Outcomes in the foundational sciences. The 2013 CAPE Outcomes perceived to be challenging to incorporate into the foundational sciences were identified. Subsequently, strategies for integration and assessment grounded in educational theories were developed.ResultsPatient advocacy (CAPE 3.3), cultural sensitivity (CAPE 3.5), innovation and entrepreneurship (CAPE 4.3), medication use management (CAPE 2.2), self‐awareness (CAPE 4.1), and leadership (CAPE 4.2) received scores suggesting limited incorporation in foundational sciences. To address this, examples of integration strategies were shown based on these 2013 CAPE Outcomes to enhance foundational science bridging with clinical and practice pharmacy education. Curricular models such as the McMaster method, H and Z stack approaches, and the Harden's Ladder as an assessment tool to measure curricular integration are examined in the context of the 2013 CAPE Outcomes, grounded in educational theory.ConclusionThis work summarizes the recommendations of the AACP Biological Sciences Section Task Force for examples of methods for achieving, augmenting, and assessing the integration of pharmaceutical and foundational sciences with clinical practice in PharmD programs.
Pharmacist‐prescribed hormonal contraception services: A systematic review of implementation studies
Authors: Newlon Jenny L. , Reed Jason B. , Stone Rebecca H. , Satterfield Kennedi G. , Meredith Ashley H. 
Published: October 2021

AbstractAs of July 2021, 16 states and Washington DC permit pharmacist prescribing of hormonal contraceptives. The objective of this systematic review is to describe the impact of pharmacist prescribing on hormonal contraceptive access in the United States (U.S.). Using a protocol based on PRISMA guidelines, a librarian performed the literature search for articles published between January 1, 2013 and January 15, 2021. Studies were excluded if they were incomplete (ie, without results) or the full text was not available. Studies that took place outside of the U.S., within the U.S. but in a state that had not implemented pharmacist contraception prescribing at the time the research was initiated, or not written in English, as well as those that were not primary literature (ie, reviews, editorials, opinion papers, etc.) were also excluded from this review. Articles were categorized based on whether the outcomes evaluated (a) uptake of pharmacist‐prescribed hormonal contraceptive services, (b) implementation of pharmacist‐prescribed hormonal contraceptive services, or (c) impact of pharmacist‐prescribed hormonal contraceptive services on patients' access to hormonal contraception. Of 2056 identified articles, 21 articles met the inclusion criteria and were included in the analysis. The majority (n = 16) assessed uptake of the service, whereas eight examined implementation and three evaluated impact of the service on patients' access. More research is warranted on the impact of pharmacist prescribing on patients' access to hormonal contraceptives.
Confronting opioid use disorder through virtual interprofessional education
Authors: Fusco Nicholas M. , Ohtake Patricia J. 
Published: October 2021

AbstractIntroductionInterprofessional education (IPE) and collaborative practice (IPCP) are requirements of most health professions education curricula and may be an important method to promote an interprofessional approach to treating opioid use disorder (OUD). The objective of this study was to evaluate the quality of a large‐scale, virtual IPE Forum on the topic of OUD and its impact on student pharmacists' assessment of their interprofessional behaviors.MethodsStudents completed a virtual IPE Forum which consisted of asynchronous online pre‐work and a live, virtual small‐group case‐based discussion activity. Within 2 weeks after participating in the activity, students completed a survey that included a program evaluation and a self‐assessment of IPCP behaviors (Interprofessional Collaborative Competency Attainment Scale [ICCAS]). Paired sample t tests determined differences between pre‐ and post‐Forum ICCAS assessments. Additionally, faculty facilitators of the small‐group activity completed a post‐program evaluation.ResultsThere were 105 third‐year student pharmacists that participated in the virtual IPE Forum. A total of 103 (98%) students completed the ICCAS instrument. There were statistically significant increases in each item on the ICCAS, as well as a total mean ICCAS score, from pre‐ to post‐activity. Most students (>86%) reported they gained new knowledge related to OUD. Most faculties (93%) reported the Forum was an effective way to teach students about OUD and IPCP. Both students and faculty rated the program highly, with most (64%) students preferring the virtual forum compared with previously offered in‐person experiences.DiscussionA virtual IPE Forum focused on OUD was successful at improving students' self‐assessed interprofessional collaborative behaviors. Additionally, student pharmacists reported they gained new knowledge related to OUD. Incorporating virtual IPE related to OUD into student pharmacists' training is beneficial and may be preferable to in‐person experiences. Future studies should compare educational outcomes of in‐person vs virtual IPE experiences and the impact such experiences have on student knowledge.
Impact of a pharmacy‐led nursing education on discharge opioid prescribing after kidney transplant
Authors: Benken Jamie , Lichvar Alicia , Reticker Anesia , Benedetti Enrico , Votta Velis Effrossyni , Campara Maya 
Published: October 2021

AbstractA major contributor to the opioid epidemic is prescribing in the postoperative setting. There remains the need for opioid stewardship both postoperatively and upon discharge in kidney transplant recipients. In October 2017, pharmacist‐led education was given to all transplant nursing staff at a single center with the goal of optimizing postoperative pain control through education and empowerment of nurses. Furthermore, the education focus was to advance nursing knowledge, enhance patient assessment, and reform patient education. Clinical pharmacists continued to work with the transplant team to base a patient's discharge analgesia on inpatient analgesia use. This study assessed the impact of the nursing education on discharge analgesic prescribing patterns after kidney transplant admission. Opioid prescribing on transplant discharge was significantly lower after the education (pre 68.3% vs post 11.1%, P <.001). Transplant admission was shortened by 1 day (6 vs 5 days, P = .03). Over time, a significant downtrend in opioid prescribing was observed on discharge from 86.1% in 2015 to 49.6% in 2017 and 8.5% in 2018 (P <.001). If opioid therapy was required on discharge in the posteducation group, tramadol was predominantly prescribed (7/13 opioid prescriptions, 53.9%). Thus, opioid minimization and pain management using nonopioid analgesic prescribing on discharge are feasible in an adult kidney transplant population with proper nursing collaboration and education.
Toward allogenizing a xenograft: Xenogeneic cardiac scaffolds recellularized with human‐induced pluripotent stem cells do not activate human naïve neutrophils
Authors: Al‐Hejailan Reem S. , Bakheet Razan H. , Al‐Saud Mashael M. , Al‐Jufan Mansour B. , Al‐Hindas Hussain M. , Al‐Qattan Somaya M. , Al‐Muhanna Muhanna K. , Parhar Ranjit S. , Conca Walter , Hansmann Jan , Collison Kate S. , Walles Heike , Al‐Mohanna Futwan A. 
Published: October 2021

AbstractThe limited availability of human donor organs suitable for transplantation has resulted in ever‐increasing patient waiting lists globally. Xenotransplantation is considered a potential option, but is yet to reach clinical practice. Although remarkable progress has been made in overcoming immunological rejection, issues with functionality are still to be resolved. Bioengineering approaches have been used to create cardiac tissues with optimized functions. The use of decellularized xenogeneic cardiac tissues seeded with donor‐derived cardiac cells may prove to be a viable strategy as supporting structures of the native tissue such as vasculature can be utilized. Here we used sequential perfusion to decellularize adult rat hearts. The acellular scaffolds were reseeded with human endothelial cells, human fibroblasts, human mesenchymal stem cells, and cardiac cells derived from human‐induced pluripotent stem cells. The ability of the resultant recellularized rat scaffolds to activate human naïve neutrophils in vitro was investigated to measure xenogeneic recognition. Our results demonstrate that in contrast to cadaveric xenogeneic hearts, acellular and recellularized xenogeneic scaffolds did not activate human naïve neutrophils and suggest that decellularization removes the xenogeneic antigens that lead to human naïve neutrophil activation thus allowing human cells to populate the now “allogenized” xenogeneic scaffolds.
Functional differences between Hsp105/110 family proteins in cell proliferation, cell division, and drug sensitivity
Authors: Teshima Hiroko , Watanabe Hiroko , Yasutake Ryuji , Ikeda Yuki , Yonezu Yukiko , Okamoto Namiko , Kakihana Ayana , Yuki Ryuzaburo , Nakayama Yuji , Saito Youhei 
Published: October 2021

AbstractThe mammalian HSP105/110 family consists of four members, including Hsp105 and Apg‐1, which function as molecular chaperones. Recently, we reported that Hsp105 knockdown increases sensitivity to the DNA‐damaging agent Adriamycin but decreases sensitivity to the microtubule‐targeting agent paclitaxel. However, whether the other Hsp105/110 family proteins have the same functional property is unknown. Here, we show that Apg‐1 has different roles from Hsp105 in cell proliferation, cell division, and drug sensitivity. We generated the Apg‐1‐knockdown HeLa S3 cells by lentiviral expression of Apg‐1‐targeting short hairpin RNA. Knockdown of Apg‐1 but not Hsp105 decreased cell proliferation. Apg‐1 knockdown increased cell death upon Adriamycin treatment without affecting paclitaxel sensitivity. The cell synchronization experiment suggests that Apg‐1 functions in mitotic progression at a different mitotic subphase from Hsp105, which cause difference in paclitaxel sensitivity. Since Apg‐1 is overexpressed in certain types of tumors, Apg‐1 may become a potential therapeutic target for cancer treatment without causing resistance to the microtubule‐targeting agents.
Reference values of urine protein/creatinine ratio in healthy Dalian adults
Authors: Liu Tong , Xue Bang‐Lu , Du Bin , Cui Tong , Gao Xin , Wang Yue‐Ying , Wang Bo , Wei Jin‐Long 
Published: October 2021

AbstractBackgroundThe urine protein/creatinine ratio (UPCR) is commonly used in current clinical practice. However, there are only few published clinical data on UPCR from large cohorts of Chinese adults. This study aimed to determine the overall and age‐ and sex‐specific UPCR reference values for healthy Dalian adults.MethodsAccording to the Clinical & Laboratory Standards Institute EP28‐A3c guidelines, 1321 healthy Dalian adults (646 men and 675 women) aged 20–69 years were enrolled. Urine protein and creatinine levels were analyzed in the random morning spot urine samples, and UPCR was calculated. The 95th percentile of the UPCR was used as the normal upper limit. The Mann–Whitney U test was used to test differences among groups.ResultsThe UPCR reference value was 141.7 mg/g for the entire cohort, 128.7 mg/g for men, and 150.8 mg/g for women. In addition, women had relatively higher UPCR values than men in the same age group. We also compared the UPCR reference values between different estimated glomerular filtration rate (eGFR) groups and found that women had significantly higher UPCR values than men in the normal eGFR groups.ConclusionsThis study provides the overall and age‐ and sex‐specific UPCR reference values for healthy Dalian adults.
Changes in systemic GDF15 across the adult lifespan and their impact on maximal muscle power: the Copenhagen Sarcopenia Study
Authors: Alcazar Julian , Frandsen Ulrik , Prokhorova Tatyana , Kamper Rikke S. , Haddock Bryan , Aagaard Per , Suetta Charlotte 
Published: October 2021

AbstractBackgroundAlthough growth differentiation factor 15 (GDF15) is known to increase with disease and is associated with low physical performance, the role of GDF15 in normal ageing is still not fully understood. Specifically, the influence of circulating GDF15 on impairments in maximal muscle power (a major contributor to functional limitations) and the underlying components has not been investigated.MethodsData from 1305 healthy women and men aged 20 to 93 years from The Copenhagen Sarcopenia Study were analysed. Circulating levels of GDF15 and markers of inflammation (tumor necrosis factor‐alpha, interleukin‐6, and high‐sensitivity C‐reactive protein) were measured by ELISA (R&D Systems) and multiplex bead‐based immunoassays (Bio‐Rad). Relative (normalized to body mass), allometric (normalized to height squared), and specific (normalized to leg muscle mass) muscle power were assessed by the Nottingham power rig [leg extension power (LEP)] and the 30 s sit‐to‐stand (STS) muscle power test. Total body fat, visceral fat, and leg lean mass were assessed by dual energy X‐ray absorptiometry. Leg skeletal muscle index was measured as leg lean mass normalized to body height squared.ResultsSystemic levels of GDF15 increased progressively as a function of age in women (1.1 ± 0.4 pg·mL−1·year−1) and men (3.3 ± 0.6 pg·mL−1·year−1) (both P < 0.05). Notably, GDF15 increased at a faster rate from the age of 65 years in women (11.5 ± 1.2 pg·mL−1·year−1, P < 0.05) and 70 years in men (19.3 ± 2.3 pg·mL−1·year−1, P < 0.05), resulting in higher GDF15 levels in men compared with women above the age of 65 years (P < 0.05). Independently of age and circulatory markers of inflammation, GDF15 was negatively correlated to relative STS power (P < 0.05) but not LEP, in both women and men. These findings were mainly explained by negative associations of GDF15 with specific STS power in women and men (both P < 0.05).ConclusionsA J‐shaped relationship between age and systemic GDF15 was observed, with men at older age showing steeper increases and elevated GDF15 levels compared with women. Importantly, circulating GDF15 was independently and negatively associated with relative STS power, supporting the potential role of GDF15 as a sensitive biomarker of frailty in older people.
Kinetıc analysıs of Cr(VI) transport wıth electromembrane processes
Authors: Çalık Gamze , Kaya Ahmet , Onac Canan , Aytaç Aylin , Alpoguz Hamza Korkmaz 
Published: October 2021

AbstractBACKGROUNDIn this study, the transport of Cr (VI) ions was investigated with electroanalytical approach, called electro‐membrane extraction (EME), by using a supported liquid membrane (SLM).RESULTSAliquat 336 and Celgrad 2500 were used as a carrier and support material, respectively. Chromium (VI) ions were transferred from sample solution containing target analyte to extractant solution at low potential values (10–50 V) and with different current values (0.1–0.4 A).CONCLUSIONThe following parameters such as carrier change, carrier concentration effects, solvent change effect were investigated and Cr(VI) transport was achieved with 54.73% recovery in 100 min at optimum conditions. In the EME‐SLM process, Danesi mass transfer model was used to calculate the kinetic data, rate constant, flux, permeability, and recovery, for each parameter examined. © 2021 Society of Chemical Industry (SCI).
Lactic acid production from food waste using the lactogenic Escherichia coli strain JU15: optimization of reducing sugar recovery
Authors: Santos‐Corona Anaid M , Lázaro‐Mixteco Pedro E , Vargas‐Tah Ana Alejandra , Castro‐Montoya Agustín J 
Published: October 2021

AbstractBACKGROUNDd‐Lactic acid (DLA) has numerous industrial applications, including production of biodegradable polylactic acid, which is one of the most promising biodegradable polymers. Looking for alternative feedstocks is a high priority and recent focus has been on its biological synthesis and biotechnological valorization from food waste (FW) materials. In this study, a response surface methodology (RSM) approach and central composite design (CCD) were performed to hydrolyze FW, obtaining released reducing sugars (RRS) for DLA production using the lactogenic Escherichia coli strain JU15.RESULTSAfter an optimized sulfuric acid‐based pretreatment methodology using RSM and CCD, FW was utilized to obtain the maximum concentration of RRS, obtaining liquors containing glucose (85.59%), fructose (5.48%) and arabinose (8.92%) with an efficiency of ∼98% total sugar conversion from hydrolysis of FW. As the total sugar sources obtained titers of 26.85 ± 1.28 g L−1 (DNS) and DLA production was 22.66 ± 1.01g L−1, the DLA yield was 0.99 g g −1 total sugar. While arabinose was discretely consumed, with a remaining concentration of 0.36 ± 0.02 g L−1 after culture, the production of DLA through bacterial fermentation of the FW hydrolysates by the JU15 strain showed total depletion of glucose and fructose, achieving a maximum DLA production of 22.66 g L−1, with a maximum volumetric productivity of 0.73 g L−1 h−1 at 36 h.CONCLUSIONThis study provides an experimental optimization of a pretreatment to obtain released reducing sugars to lactic acid production from food waste as feedstock using the lactogenic E. coli strain JU15. © 2021 Society of Chemical Industry (SCI).