bluuebunny/crossref_metadata_2025_split huggingface dataset:
142 Open Science: Improving Access and Reducing Bias in Science
Authors: Fox Nick 
Published: October 2021

Abstract
The promise of science lies in the discovery of basic knowledge, new treatments for disease and possible solutions to the world’s problems. Fulfilling this promise requires confidence that the findings of published science are valid—that they represent an unbiased conclusion based on available data. In recent years, however, a “reproducibility crisis” has emerged indicating that published findings across research fields may be less credible than they seem, perhaps due to hidden biases in the research process. This talk will provide an overview of the key challenges that reduce the credibility and reproducibility of research and will discuss how open science practices address these challenges. Current practice is sustained by a dysfunctional incentive structure that prioritizes publication over accuracy. Changing the research culture to prioritize “getting it right” over “getting it published” requires nudges to the incentive landscape, while still fueling the engine of innovation and discovery that drives science into new domains.
169 Estimating the Environmental Impact of Livestock Operations Using the Canadian Whole-farm Model Holos Developed for Canadian Farmers
Authors: Kroebel Roland , Alemu Aklilu , Pogue Sarah , McPherson Aaron 
Published: October 2021

Abstract
The Holos model is a Canadian whole-farm model that uses IPCC Tier II emission factors to calculate greenhouse gas (GHG) emissions from Canadian farming systems. These Tier II emission factors are Canada-specific with respect to land-based nitrous oxide emission, but are universal with respect to the livestock calculation. Here, however, Tier II is limited to ruminant animals, as so far only Tier I models are available for monogastrics. The model is designed to permit farmers to enter readily available farm management information themselves in order to calculate GHG sources and management driven mitigation practices. The presentation will provide an overview of the model and its design (interface and algorithms), and will showcase some of the scientific studies (cradle-to-farmgate) that were accomplished using the Holos model for assessing the carbon footprint of Canadian beef and dairy production systems.
170 Effectiveness of On-line Webinars for Delivering Extension Education to Beef Producers Throughout the COVID-19 Pandemic
Authors: VanValin Katherine , Bullock Darrh , Anderson Leslie , Laurent Kevin , Lehmkuhler Jeffrey W 
Published: October 2021

Abstract
In response to the COVID-19 pandemic, all in-person Extension programs in Kentucky were canceled in March 2020. In order to continue reaching beef producers, a webinar series was developed. The initial series was eight weekly evening sessions. Sessions were streamed live on a video meeting and social media platform and were recorded for future viewing. Programs consisted of a presentation followed by a question-and-answer session, covering multi-disciplinary topics related to beef management. Following each session, participants were asked to complete a survey to gauge the effectiveness of the programs using a series of questions with responses on a 5-point Likert scale. The average number of responses was 17 and ranged from 4–31 respondents per session. Knowledge level before and after the presentation was asked for a given topic to assess the increase in subject awareness/understanding (1: not very knowledgeable; 5: very knowledgeable). The average response across all respondents (n = 138) for pre-program knowledge was 2.98, and the average response post-program was 4.37. This represented a 46.6% change across all topics covered in the post-attendance surveys. The most common age demographic represented by survey respondents was 50–59 years of age, and 84% of respondents reported previously participating in a webinar. Participants were asked to evaluate the webinar technology in terms of its ease of access (1: not very easy; 5: very easy), and the average response was 4.64. Participants were asked to evaluate the material presented in each session for its usefulness (1: not very useful; 5- very useful), and the average response was 4.71. When asked if the webinar was an effective use of time (1: not very useful; 5: very useful), the average response was 4.8. Overall, webinars were an effective means of delivering Extension education related to beef cattle management in the midst of the COVID-19 pandemic.
176 Evaluating Dual-purpose Wheat Varieties in the Southeastern U.S
Authors: West Micayla H , Carrell Russell C , Dillard Sandra L 
Published: October 2021

Abstract
Dual-purpose wheat (Triticum aestivum L.) systems increase farm sustainability by diversifying on-farm income. While these systems are common in the Southern Great Plains of the United States, they are not often utilized in the Southeast. This study aimed to evaluate pre- and post-grazing herbage mass (HM) of four winter wheat varieties managed under a dual-purpose grazing and grain production system. The winter wheat varieties evaluated were generic feed-type wheat (mixed variety, Feed), seed-type wheat (‘GA Gore’) and two forage-type varieties, ‘AGS 2024’ (AGS) and ‘Pioneer 26R41’ (Pioneer). The experiment was a completely randomized block design with n = 4. Each plot was randomly assigned either as no-grazing (CON) or grazing (GF2). Plots were grazed with cow-calf pairs that were fasted 24 h before each grazing event. Grazing was considered complete when the average stubble height was 10 cm. Herbage mass was determined using three 0.1m2-quadrats per plot and clipping to a 10 cm stubble height before (PreG) and after (PostG) each grazing event. Forage samples were then dried at 45°C for 72 h. Data were analyzed using PROC GLIMMIX of SAS (SAS Inst., Cary, NC). Differences were declared at P &lt; 0.05. Initial HM was greater for PreG than PostG (883 and 615 kg/ha, respectively; P &lt; 0.01). Prior to grazing, AGS (1204 kg/ha) was greater (P &lt; 0.02) than all other varieties. Pre-grazing, there were no differences among the other varieties (776 kg/ha; P &gt; 0.14). Post-grazing, AGS had a greater HM than Seed (788 and 391 ka/ha, respectively; P = 0.04), while all other varieties were intermediate (642 kg/ha). Herbage mass was affected by grazing frequency with CON being greater (P &lt; 0.01) than GF2 (993 and 691 kg/ha, respectively). These results indicate that both wheat variety and grazing treatment had an effect on dual-purpose wheat herbage mass.
180 Effects of Forage Mixtures on Rumen Fermentation Parameters as Assessed by Continuous Culture
Authors: Snider Miriam A , Johnson Jacqueline M , Ziegler Sara E , Darby Heather M , Greenwood Sabrina L 
Published: October 2021

Abstract
The impact of using diverse forage mixtures on rumen performance is poorly characterized. The objective of this study was to evaluate the impact of feeding diverse pastures on rumen performance using in vitro continuous culture fermenters. Treatments were assessed using a 4 x 4 Latin square design. Each 10-d period included 7-d of adaption followed by 3-d of sample collection. Dietary treatments (DM basis) included 1) 50% orchardgrass + 50% alfalfa (OG-ALF); 2) 50% OG-ALF + 50% red clover (RC); 3) 50% OG-ALF + 50% pearl millet (MIL); and 4) 50% OG-ALF + 50% sorghum x sudangrass (SUD). Forage diets (131 g DM/ fermenter/ d) were divided into 4 portions/d (33%, 0600 h; 17%, 0720 h; 33%,1800 h; 17%, 1920 h). Fermenter pH measurements were collected every min for the entire experiment. Methane concentrations were measured using a gas probe and recorded twice daily (0530 h,1730 h) in triplicate on d 7, 8, 9, and 10. Daily total effluent samples were collected on d 8, 9, and 10 for VFA analysis. Results were analyzed using PROC MIXED of SAS. There were no differences in total VFA concentrations (P = 0.08), molar proportions (P = 0.22), or individual VFA ratios (P &gt; 0.05) as a result of treatment. Fermenter pH did not differ between fermenters as a result of treatment (P &gt; 0.05). Fermenters receiving OG-ALF had the greatest methane concentration (50.8 mg/dL), which was higher (P &lt; 0.05) than methane concentrations in fermenters receiving MIL (6.2 mg/dL), SUD (6.9 mg/dL), or RC (21.2 mg/dL). Methane concentrations from the MIL, SUD and RC treatments did not differ (P &gt; 0.05). This indicates that binary forage mixtures may have lower nutritional value compared with diverse mixtures, and this diversification could provide nutritional benefits.
189 Techniques in Teaching Forages to Animal Scientists
Authors: Naumann Harley D 
Published: October 2021

Abstract
How do you teach a plant-based course focused on forage physiology, production and management to an animal science student? How do you engage them and keep them engaged? Empathy. Put yourself in the shoes of the animal science student who is sitting in a plant-science course. Make it applied and relatable to the animal scientist. In this talk I will take a case-study approach to sharing my experience as an animal science student tasked with knowing something about plants and how I use that experience to guide my teaching methods in a Forages course at the University of Missouri.
191 Pulsatile Administration of Leucine Promotes mTOR Signaling and Protein Synthesis in Skeletal Muscle of Continuously Fed Preterm Pigs
Authors: Rudar Marko , Suryawan Agus , Nguyen Hanh , Stoll Barbara , Burrin Douglas , Fiorotto Marta , Davis Teresa A 
Published: October 2021

Abstract
Continuous feeding does not elicit an optimal anabolic response but is required for some premature infants. We reported previously that intermittent intravenous pulses of Leu (800 μmol Leu·kg-1·h-1 every 4 h) to continuously fed pigs born at term enhanced skeletal muscle mTOR signaling and protein synthesis (PS). The objective of this study was to determine if the anabolic response of skeletal muscle to intermittent Leu pulses is altered following premature birth. Pigs delivered 10 d preterm by cesarean section were advanced to full oral feeding over four days (195 kcal·kg-1·d-1; 13.5 g protein·kg-1·d-1). Pigs were assigned to 1 of 4 treatments: 1) ALA (continuous feeding; 800 μmol Ala·kg-1·h-1 every 4 h; n = 7); 2) L1× (continuous feeding; 800 μmol Leu·kg-1·h-1 every 4 h; n = 6); 3) L2× (continuous feeding; 1600 μmol Leu·kg-1·h-1 every 4 h; n = 6); and 4) INT (intermittent feeding every 4 h; n = 5). Pigs received a Phe tracer 30 min following the amino acid pulse or intermittent feeding to measure PS in longissimus dorsi muscle. Anabolic signaling downstream of mTOR was determined by immunoblot.ResultsPS was higher in L2× and INT compared to ALA (P &lt; 0.05) but was not different between L2× and INT; PS in L1× was intermediate and not different from other groups. Phosphorylation of 4EBP1 and S6K1 was higher in INT compared to L1× and ALA groups (P &lt; 0.05) but was not different compared to L2×. Phosphorylation of 4EBP1, but not S6K1, was higher in L2× compared to ALA (P &lt; 0.05); phosphorylation of 4EBP1 and S6K1 was not different between L1× and L2×.ConclusionsThese results demonstrate that intravenous Leu enhances mTOR activation and PS in LD muscle of continuously fed preterm pigs. However, the amount required may be higher than in pigs born at term.Source of Research SupportNIH and USDA.
192 Identification of Fos and FosB as Transcriptional Regulators of Bovine Satellite Cell Differentiation
Authors: Lyu Pengcheng , Settlage Robert , Jiang Honglin 
Published: October 2021

Abstract
Transcription factors (TFs) are key regulators of gene expression during cell differentiation. Four TFs including Myf5, MyoD, MyoG and Myf6 have been identified as key myogenic regulatory factors (MYFs) that regulate gene transcription during myogenesis. Satellite cells (SCs) are the myogenic precursor cells in adult skeletal muscle. The objective of this study was to identify additional TFs that control the differentiation of bovine satellite cells. Bovine satellite cells (bSCs) were isolated from 4 crossbred steers and were initially cultured in growth medium for 12 days to expand and then in differentiation medium for 48 hours to differentiate. Chromatin immunoprecipitation followed by sequencing (ChIP-seq) was performed to identify chromatin regions marked with acetylation of histone H3 on lysine 27 (H3K27ac). This ChIP-seq analysis revealed 3,348 and 38,800 H3K27ac-associated chromatin regions in bSCs before and after differentiation, respectively. A motif enrichment analysis of the H3K27ac-marked chromatin regions from the differentiated bSCs indicated the enrichment of binding sites for the 4 MYFs and many other TFs including Fos and FosB. RNA-sequencing revealed the upregulation of Fos and FosB mRNAs in bSCs from growth to differentiation. To verify the roles of Fos and FosB in bSC differentiation, their expressions in bSCs were reduced by siRNA-induced knockdown. Based on qRT-PCR analyses, expressions of MYH2, MYH3, MYOG, and CKM mRNAs, which were selected as markers of muscle cell differentiation, were increased (P &lt; 0.05) in bSCs from growth to differentiation, but the increases in at least three of them were reversed (P &lt; 0.05) by Fos or FosB knockdown. Taken together, these results establish Fos and FosB as transcriptional regulators of bovine satellite cell differentiation.
193 Single Cell RNA-sequencing Reveals a Role of Lipid Metabolism in Muscle Satellite Cells
Authors: Kuang Shihuan , Yue Feng , Oprescu Stephanie 
Published: October 2021

Abstract
Single Cell RNA-sequencing (scRNA-seq) is a powerful technique to deconvolute gene expression of various subset of cells intermingled within a complex tissue, such as the skeletal muscle. We first used scRNA-seq to understand dynamics of cell populations and their gene expression during muscle regeneration in murine limb muscles. This leads to the identification of a subset of satellite cells (the resident stem cells of skeletal muscles) with immune gene signatures in regenerating muscles. Next, we used scRNA-seq to examine gene expression dynamics of satellite cells at various status: quiescence, activation, proliferation, differentiation and self-renewal. This analysis uncovers stage-dependent changes in expression of genes related to lipid metabolism. Further analyses lead to the discovery of previously unappreciated dynamics of lipid droplets in satellite cells; and demonstrate that the abundance of the lipid droplets in newly divided satellite daughter cells is linked to cell fate segregation into differentiation versus self-renewal. Perturbation of lipid droplet dynamics through blocking lipolysis disrupts cell fate homeostasis and impairs muscle regeneration. Finally, we show that lipid metabolism regulates the function of satellite cells through two mechanisms. On one hand, lipid metabolism functions as an energy source through fatty acid oxidation (FAO), and blockage of FAO reduces energy production that is critical for satellite cell function. On the other hand, lipid metabolism generates bioactive molecules that influence signaling transduction and gene expression. In this scenario, lipid metabolism and FAO regulate the intracellular levels of acetyl-coA and selective acetylation of PAX7, a pivotal transcriptional factor underlying function of satellite cells. These results together reveal for the first time a critical role of lipid metabolism and lipid droplet dynamics in muscle satellite cell fate determination and regenerative function; and underscore a potential role of dietary fatty acids in satellite cell-dependent muscle development, growth and regeneration.
500 Late-Breaking: Heart Rate During Exercise Is Positively Correlated with State IV Mitochondrial Respiration in the Equine Skeletal Muscle
Authors: Kawaida Mia Y , Kwon Oh-Sung , Anh Ahram , Reiter Amanda S , Tillquist Nicole M , Reed Sarah A 
Published: October 2021

Abstract
Mitochondria are critical for oxidative phosphorylation in skeletal muscle, especially in athletic species such as the horse. Mitochondrial respiration increases with physical exercise, but the relationship between mitochondrial respiration and cardiovascular functions are not well described in the horse. The objective of this study was to determine if there is a relationship between heart rate (HR) during and after submaximal exercise tests (SETs) and skeletal muscle mitochondrial respiration in polo ponies. We hypothesized that horses with greater maximum HR and average HR during the exercise tests would have greater mitochondrial respiration in skeletal muscle. Twelve fit polo ponies (14.8 ± 1.7 years old, 10 mares and 2 geldings) were equipped with Polar equine heart rate monitors (Polar Electro Inc., Lake Success, NY) and underwent 26-minute SETs designed to mimic a polo chukker followed by a 30-minute recovery period. Muscle biopsy samples from the semitendinosus muscle were taken 2 weeks prior to the SET to determine mitochondrial oxygen consumption using the Oroboros O2k high-resolution respirometer (Oroboros Instruments, Innsbruck, Austria). Data were analyzed using the PROC CORR procedure (SAS Inst. Inc., Cary, NC). Correlations were considered strong at r &gt; 0.6 and significant at P &lt; 0.05. Maximum HR during SET and state IV respiration were positively correlated (P = 0.02, r = 0.68). Average HR during SET and state IV respiration were also positively correlated (P = 0.01, r = 0.72). However, correlations between maximum and average HR and state III respiration were not as strong (P ≥ 0.05, r &lt; 0.6). These data suggest that state IV mitochondrial respiration (proton leak) in equine skeletal muscle may impact cardiac responses to high-intensity exercise. Horses with higher HR during exercise may have less efficient oxidative phosphorylation, resulting in earlier fatigue and/or greater formation of reactive oxygen species resulting from proton leak.
204 Awardee Talk: Recent Advances in Protein Nutrition in Horses
Authors: Urschel Kristine 
Published: October 2021

Abstract
Protein has been recognized as an essential nutrient for animals for well over 100 years. Protein plays many important structural and metabolic roles, and some of its component amino acids have additional functions, including as regulatory molecules, as energy substrates and in the synthesis of other non-protein molecules. Skeletal muscle makes up approximately 50% of body weight in horses, with protein being the major non-water component. As an athletic species, the development and maintenance of muscle mass is of the utmost importance in horses. Because muscle mass is largely determined by the balance of rates of muscle protein synthesis and breakdown, understanding how these pathways are regulated and influenced by dietary protein and amino acid provision is essential. Historically, much research regarding protein nutrition in horses has focused on the protein digestibility of different feed ingredients, and the adequacy of different protein sources in supporting the growth and maintenance of horses. This presentation will focus on some of the current areas of active research relating to protein nutrition in horses: the activation of the signaling pathways that regulate muscle protein synthesis, amino acid supplementation in athletic horses, protein metabolism in aged and horses and those with insulin dysregulation, and amino acid and protein nutrition in predominantly forage-fed horses. There are many exciting opportunities for future research in the area of protein and amino acid nutrition in horses across the lifespan.
213 Nutritional and Genetic Influences on Fatty Acid Composition of Beef and Lamb
Authors: Duckett Susan K , Thomas Alexandra R , Udoka Aliute , Greene Maslyn A 
Published: October 2021

Abstract
There is increased competition in the protein market with the entrance of cell-based and plant-based products. Consumers issues with consumption of red meat typically center around saturated (SFA) fatty acid content and environmental impacts associated with production. Nutritional system used for finishing beef and lamb alters fatty acid composition of the meat product. Forages and grasses that are utilized in a grass-fed finishing system increases the animal’s intake of a-linolenic acid (C18:3 n-3), the main fatty acid present in plants (57%) that can be converted to eicosapentaenoic (C20:5 n-3), docosapentaenoic (C22:5 n-3) and docosahexaenoic (C22:6 n-3) acids in animal tissues. Grain-fed finishing systems increases the animal’s intake of linoleic acid (C18:2 n-6), the main fatty acid present in corn grain (57%), that can be converted to arachidonic acid (C20:4 n-6) and docosatetraenoic acid (C22:4 n-6) in animal tissues. Biohydrogenation of polyunsaturated fatty acids (PUFA) is extensive in the rumen (&gt; 80% of dietary PUFA) and rumen protected supplements are needed to enhance PUFA content of ruminant meat products. Saturated (SFA) and monounsaturated (MUFA) fatty acids are the majority (&gt;75% of total fatty acids) of fatty acids present in ruminant meat products. Therefore, finding approaches to attain greater desaturation of palmitic and stearic acids to their MUFA products, palmitoleic and oleic acids, would be most beneficial. The content of oleic acid and MUFA in beef is highly heritable and some breeds have high levels of MUFA. Stearoyl-CoA desaturase (SCD1) is the rate limiting enzyme in the production of MUFA. More research is needed to find ways of altering SCD1 expression in meat animal tissues to enhance MUFA and lower SFA content.
226 Integrated Pork Production During a Global Pandemic: Impact on the Live Production Supply Chain, Feed Mills, & Nutrition
Authors: Parks Chris , Shipp Tommy , Hoogland Marlin , Cordova Alejandro 
Published: October 2021

Abstract
The global COVID-19 pandemic of 2020/2021 resulted in widespread impacts on the production & processing of animal proteins. Specifically, the U.S. pork industry was hit with multiple, long-term plant closures and slow-downs due to labor safety issues and availability, resulting in a backlog reaction felt throughout the live production supply-chain. Impact and timing differed by region and required variable strategies to address them. While some plants in the Western United States weren’t impacted until the latter stages of the pandemic and thus were attempting to add valuable liveweight, other regions, including the Midwest and Southeastern United States were experiencing moderate to severe reductions in processing capacity that created an accumulation of heavy animals and placed additional feed and ingredient demands on the feed mills. The backlog also reduced available space & disrupted the normal barn fill/empty cycles. While there was no way to prepare for these impacts, university & industry groups attempted to fill the knowledge gap with management and nutritional tools to address the issues of excess inventory, decreasing feed capacity, and supply-chain disruptions. Numerous approaches were employed, including altered grading strategies, sow breeding target reductions, double/triple stocking situations, and feed, ingredient, and nutrient manipulation meant to slow down growth rate and feed intake. Nutritional strategies included the feeding of low energy ingredients as well as low protein/high methionine diets designed to reduce the growth rate of both heavy and light animals. While several of these strategies resulted in moderate success, some resulted in additional issues including stress-related vices, ulcers, increased mortality, and sub-optimal carcass characteristics. Ultimately, continuous planning, coordination, and communication between the plants, feed mills, nutritionists, and production groups enabled a return to normalcy, and while we hope to never experience a pandemic again, we are left with knowledge that will improve the resilience of the U.S. pork industry.