bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evidence for Basolateral P2Y6 Receptors along the Rat Proximal Tubule
Authors: BAILEY MATTHEW A. , IMBERT-TEBOUL MARTINE , TURNER CLARE , SRAI S. KAILA , BURNSTOCK GEOFFREY , UNWIN ROBERT J. 
Published: April 2021

Abstract.
In this study, the distribution of P2Y6 receptor mRNA in rat nephron segments was investigated and a functional approach was used to analyze basolateral protein expression. Reverse transcription-PCR studies revealed more intense expression of P2Y6 receptor mRNA in the proximal tubule and the thick ascending limb of Henle's loop, less intense expression in the thin descending limb and the cortical and outer medullary collecting ducts, and no detectable expression in either the thin ascending limb or the inner medullary collecting duct. Dose-dependent calcium responses to basolateral administration of UDP (a selective agonist for the P2Y6 receptor) were observed in the proximal tubule but not in any of the other segments studied. In the proximal tubule, intracellular calcium concentration changes induced by UDP were associated with increased production of inositol phosphates, as were those induced by ATP and norepinephrine. However, UDP-induced intracellular calcium concentration changes were different, exhibiting no plateau after the initial peak; moreover, a single stimulation with a high concentration of UDP induced full desensitization of the UDP-sensitive calcium pathway but did not alter the responsiveness of the proximal tubule to ADP (a specific P2Y1 receptor agonist), ATP or norepinephrine. In summary, this report demonstrates that P2Y6 receptor mRNA is expressed in most segments of the rat nephron but that basolateral expression of the protein is restricted to the proximal tubule, where the receptor is coexpressed with the P2Y1 receptor. The differences in the distributions of P2Y6 receptor mRNA and UDP responses may indicate the presence of luminal receptors in other nephron segments.
Role of Mast Cell Tryptase in Renal Interstitial Fibrosis
Authors: KONDO SHUJI , KAGAMI SHOJI , KIDO HIROSHI , STRUTZ FRANK , MU[Combining Diaeresis]LLER GERHARD A. , KURODA YASUHIRO 
Published: April 2021

Abstract.
Renal interstitial fibrosis is characterized by increased proliferation of fibroblasts and excessive accumulation of extracellular matrix. Mast cell tryptase has been implicated in the development of tissue fibrosis in skin and lungs. However, the significance of mast cell tryptase in human renal diseases has not been investigated. The potential role of mast cell-derived tryptase in the development of renal fibrosis was studied using immunohistochemical techniques and cultured human renal fibroblast cell lines. Semiquantitative immunostaining analysis of samples from 70 patients with several renal diseases, including IgA glomerulonephritis (GN) (n = 30), non-IgA GN (n = 5), membranous GN (n = 5), focal segmental glomerulosclerosis (n = 4), minor glomerular abnormalities (n = 5), lupus nephritis (n = 3), and acute or chronic tubulointerstitial nephritis (n = 18), revealed that the degree of renal interstitial fibrosis was well correlated with the number of infiltrating tryptase-positive mast cells (P < 0.01). Mast cells could not be detected in damaged glomeruli in any form of renal disease. [3H]Thymidine uptake experiments demonstrated that DNA synthesis by cultured renal fibroblasts was increased with the concentration of tryptase (0.5 to 5 nM) coincubated with heparin and was suppressed by coincubation with the protease inhibitors leupeptin and benzamidine hydrochloride. Tryptase alone also increased DNA synthesis by fibroblasts but exhibited less effectiveness, compared with the combination of tryptase and heparin. Conversely, heparin alone suppressed DNA synthesis by fibroblasts. Metabolic [35S]methionine-labeling experiments with cultured renal fibroblasts indicated that tryptase increased the synthesis of fibronectin and collagen type I, in a dose-dependent manner. These findings suggest that mast cell tryptase plays a role in the proliferation and extracellular matrix protein production of renal interstitial fibroblasts and thus contributes to the development of renal interstitial fibrosis.
PAX2 Gene Mutation in a Family with Isolated Renal Hypoplasia
Authors: NISHIMOTO KAORI , IIJIMA KAZUMOTO , SHIRAKAWA TAKU , KITAGAWA KOUSAKU , SATOMURA KENICHI , NAKAMURA HAJIME , YOSHIKAWA NORISHIGE 
Published: April 2021

Abstract.
The PAX2 gene encodes a transcription factor that plays a critical role in the development of the urogenital tract, eyes, ears, and central nervous system. Recently, renal hypoplasia was observed to be part of the renal-coloboma syndrome, which is caused by heterozygous mutations of the PAX2 gene. The renal-coloboma syndrome is a rare autosomal dominant syndrome that involves optic nerve colobomas and renal anomalies. For investigation of whether PAX2 mutations occur in patients with isolated renal hypoplasia, patient DNA was analyzed for PAX2 mutations, by using PCR and direct sequencing. The study involved 20 patients with bilateral renal hypoplasia associated with decreased renal function. Heterozygous PAX2 mutations were detected in two patients, i.e., a novel nonsense mutation (C to A transversion at position 1566 in exon 9) in patient 1 and another novel nonsense mutation (C to T transversion at position 1318 in exon 7) in patient 2. The nucleotide changes for patients 1 and 2 directly introduced stop codons, presumably resulting in a message for a truncated PAX2 protein that lacked a partial transactivation domain. An ophthalmologic examination revealed a very mild, asymptomatic coloboma in patient 2, whereas the fundus was normal for patient 1. The mutation cosegregated with the presence of renal hypoplasia in the family of patient 1, appearing de novo in the mother of the patient, which strongly suggests that this mutation was the cause of renal hypoplasia in this family. This study demonstrates for the first time that PAX2 mutations can be responsible for isolated renal hypoplasia.
Furniture from Antoni Gaudi’s Guell Palace and its Connection with the Design
Authors: Miquel Mónica Piera 
Published: April 2021

This paper offers new data on the furniture designed by Antoni Gaudí for the Güell palace in Barcelona (start in 1886) some integrated into the architecture and others exempt. The studdy offers new data about the furniture as well as an analysis of the connections between the decoration and the rest of the furniture, part of which came from the previous residence of the family. The relationships are shown in materials, forms and iconography. We discovered the architect’s interest in gilding, fabrics and trimmings. The focus of the study have been the furniture survived, the photos and the descriptions of the palace when the family Güell López lived there, since there is little proof in the practically empty building.
Differential potency of vitamin D3, folic acid and memantine in protecting against neurobehavioral alterations of scopolamine induced Alzheimer’s model in rats
Authors: Eldeeb Abeer A. , Fathy Amira E. , Elgendy Salwa A. 
Published: April 2021

Background: Alzheimer disease is the cause of 60% to 70% of cases of dementia in elderly people, it is a chronic neurodegenerative disease that usually starts slowly and worsens over time. AD is characterized by the presence of senile plaques enriched with insoluble aggregate of beta-amyloid, neurofibrillary tangles and cholinergic neuronal degeneration in the brain tissue, leading to neural dysfunction, neuroinflammation, and critical pathological perturbations.Methods: Thirty-six males were classified into control group, Alzheimer-induced model (scopolamine 2.5 mg/kg IP once daily for 21 days). Folic acid-treated group (4 mg/kg, IP) once daily for 21 days with scopolamine. Vitamin D3-treated group (42 IU/kg, SC) once daily for 21 days with scopolamine. Vitamin D3 and folic acid-treated group (vitamin D3; 42 IU/kg, SC and folic acid; 4 mg/kg, IP) once daily with scopolamine for 21 days. Memantine-treated group (20 mg/kg IP) once daily with scopolamine for 21 days.Results: Induction of Alzheimer’s showed significant decrease in brain tissue levels of BDNF, Ach, glutathione reductase and significant increase in amyloid peptide 1-42 level with significant memory impairment, significant increase of initial acquisition latency, firstt retention latency and second retention latency. While administration of folic acid, vitamin D3, memantine separately or in combination resulted insignificant increase of brain tissue levels of BDNF, Ach, glutathione reductase with significant reduction of amyloid peptide 1-42 level with significant memory improvement (significant decrease IAL, first RL and second RL). Also showed improvement of histopathological changes occurred in the brain.Conclusions: Data obtained in the present study revealed that treatment of experimentally induced alzheimer rats with folic acid or vitamin D3 or memantine separately or combined group (folic acid+vitamin D3) resulted in significant increase of brain tissue levels of BDNF, acetyl choline, glutathione reductase with significant reduction of amyloid peptide 1-42 level with significant decrease of IAL, first RL and second RL to reach the platform with improvement of histopathological changes occurred in the brain. But combined and memantine-treated groups resulted in more significant improvement than other treated groups.
Study of the cost variation analysis of anti-epileptic drugs available in different brands in Indian pharmaceutical market
Authors: Kumar Hansraj , Kesari Uma Shankar Prasad , Kumar Rajiv 
Published: April 2021

Background: The aim of this study was to analyze the cost ratio and percentage cost variations in different brands of the commonly prescribed anti-epileptic drugs available in Indian pharmaceutical market.Methods: The maximum and minimum price of each brand of the drug given in Indian rupees (INR) was noted by using CIMS January to April 2020 edition and drug today April to June 2020 volume 1. The cost ratio and the percentage cost variation for individual drug brands was calculated. The cost of one bottle in case of 100 ml syrup and 10 tablets/capsules was calculated in case of oral drugs and the cost of one 1 vial or ampoule was noted in case of injectable drugs. At last the cost ratio and percentage cost variation of various brands was compared.Results: After calculation of cost ratio and percentage cost variation for each brand of anti-epileptic drug tablet clonazepam (2 mg) shows highest cost ratio and percentage cost variation as 10.41 and 941.66, carbamazepine (200 mg SR tablet) shows lowest cost ratio and percentage cost variation as 1.09 and 9.32.Conclusions: Epilepsy is the most common neurological disorder and epileptic drugs are to be prescribed for prolonged period. If a costly brand is prescribed, the patients have to pay more money unnecessarily for their treatment. There is a wide difference in the cost of different brands of anti-epileptic drugs available in India. The clinicians prescribing these drugs should be aware of these variations in cost to reduce the cost of drug therapy.
Irrational prescribing: myths and facts
Authors: Saravanabavan Nandhini , R. Sudar Codi , K. Manimekalai 
Published: April 2021

According to WHO, irrational prescribing is a disease which is difficult to treat but prevention is possible. There are many factors which contributes for this irrational prescribing like patient factors, practitioners/doctor factors, influence from pharmaceutical industry or combination of all.
Does local implantation of gentamicin impair renal function in patients undergoing surgery for chronic bone infection?
Authors: Muir Ross , Birnie Catherine , Hyder-Wilson Robert , Ferguson Jamie , McNally Martin A. 
Published: April 2021

<p><strong>Background: </strong>The treatment of chronic bone infection often involves excision of dead bone and implantation of biomaterials which elute antibiotics. Gentamicin is a preferred drug for local delivery, but its systemic use carries a well-established risk of nephrotoxicity. We aim to establish the risk of acute kidney injury (AKI) with local delivery in a ceramic carrier.</p><p><strong>Methods: </strong>163 patients with Cierny-Mader type 3 or 4 chronic osteomyelitis had a single-stage operation including filling of the osseous defect with a calcium sulphate-hydroxyapatite carrier containing gentamicin. Mean gentamicin dosing was 191.3 mg (maximum 525 mg). Glomerular filtration rate (GFR) was calculated pre-operatively and during the first seven days post-operatively. Renal impairment was graded using the chronic kidney disease (CKD) staging system, and AKI was assessed using the RIFLE criteria.</p><p><strong>Results: </strong>155 cases had adequate data to allow calculation of pre- and post-operative GFR. 7 had pre-existing renal disease. 70 patients (45.2%) had a temporary GFR drop post-operatively, with the greatest decrease occurring at a mean of 3.06 days following surgery. Twenty cases had a &gt;10% decline in GFR, but 12 resolved within 7 days. 7 patients transiently fell into the “Risk” category according to RIFLE criteria, but no patient had a change consistent with “Injury”, “Failure” or “Loss” of renal function and none had clinical signs of new acute renal impairment post-operatively. </p><p><strong>Conclusions: </strong>Renal function is not significantly affected by local implantation of gentamicin up to 525 mg. The presence of pre-existing renal disease is not a contraindication to local gentamicin therapy.</p><p><strong> </strong></p>
Characteristics and outcome of road traffic injured patients in the emergency department of a tertiary teaching hospital in Nigeria
Authors: Afuwape Oludolapo O. , Alonge Temitope O. , Irabor Achiaka E. , Balogun Mosi J. 
Published: April 2021

<p class="abstract"><strong>Background:</strong> The exit modes from the emergency department (ED) for road traffic injury patients are namely planned discharge; admission to the wards/intensive care unit or transfer to the operating theatre; discharge against medical advice (DAMA). The aim of this study was to assess the characteristics and outcomes of non-poly-traumatized road traffic injury (RTI) related ED admissions of a tertiary health care facility in a developing country and the exit pattern from the ED.</p><p class="abstract"><strong>Methods:</strong> This was a retrospective hospital data-based study of outcome of RTI patients seen in the ED of a tertiary teaching hospital in Nigeria from January to December 2017.<strong></strong></p><p class="abstract"><strong>Results:</strong> 1120 RTI patients were recruited consisting of 774 males and 346 females with a male: female ratio of 2.24:1. The age range was 1 to 94 years with a mean age of 37.5±17.5 years. Peak ages were in the fourth (24.2%) and third (20.4%) decades of life respectively. 85.5% of the patients were commercial vehicles passengers. Motorcycle accidents constituted 44% of the patients while 41.8% were in cars. The commonest injuries were head injuries (31% m:f 2.7) and fractures (21% m:f 3). There were 11.5% DAMA and 7.1% deaths. Motorcycles injuries had the highest mortality rate while the motorized tricycles injuries had the highest DAMA rates.</p><p class="abstract"><strong>Conclusions:</strong> Motorized tricycles may be considered as a safer means of commercial mode of transportation compared to motorcycles despite its own limitations. There is a need to educate patients against the common practice of discharge against medical advice.</p>
Study on functional outcome of bimalleolar ankle fractures treated by open reduction and internal fixation
Authors: Gangadhran Nithin , Pillai Manju G. 
Published: April 2021

<p class="abstract"><strong>Background:</strong> Ankle injury is the most common weight bearing orthopaedic musculoskeletal trauma encountered in emergency medicine and practice. Ankle joint is highly congruous and any disturbance of normal articular relationship may result in some progressive arthrosis of biomechanical dysfunction. As with all intra-articular fractures it necessitates accurate reduction and stable internal fixation. The objectives were to study the functional outcome of surgical treatment of bimalleolar ankle fractures and to know the complications of open reduction internal fixation of bimalleolar fractures.</p><p class="abstract"><strong>Methods:</strong> 45 patients with malleolar fractures were included in this prospective longitudinal interventional study. Patients who underwent operative treatment were followed up regularly for 6 months with OPD visits and X-ray imaging at each stage. Patient parameters were recorded at immediate post op period, 6 weeks, 12 weeks and 24 weeks. Baird and Jackson scoring system for ankle were used for the functional outcome measurement.<strong></strong></p><p class="abstract"><strong>Results:</strong> Most common type of injury pattern was supination-external rotation with 21 cases (47% of cases). The results are excellent to good in 65% of patients, 27% of patients had fair and 8% had poor result. Syndesmotic screw fixation was done with 4.5 mm cortical screw in 7 cases. Most common complication was surgical site infection in 3 cases (6.67%). 2 patients underwent implant removal due to unresolved infection at 3 months.</p><p class="abstract"><strong>Conclusions:</strong> The results of operative fixation were satisfactory in 90% of patients. Most of the complications were minor and resolved within three weeks.</p><p class="abstract"> </p>
Assessment of the functional outcome of multiple discs prolapses treated with discectomy and surgical stabilization in tertiary care hospital in Salem district
Authors: Jose Leo , K. Soundararajan , S. Aldrin Shalom , Manoharan A. E. 
Published: April 2021

<p><strong>Background: </strong>Low back pain affect up to 80% of people in the modern world at some point in their lives, creating high rates of activity limitation, work absence, impaired quality of life, and the need for medical care. To assess the functional outcome of patients with multiple discs prolapses treated with discectomy and surgical stabilization.</p><p><strong>Methods: </strong>A longitudinal study was conducted in the orthopedics department of Vinayaka missions Kirupananda Variyar medical college, Salem for two years between August 2019 and July 2020. A total of 30 patients presenting with lumbar disc herniation with radiculopathy and neurological deficits were included in the study. The diagnosis was confirmed by doing an X-ray and MRI of the lumbosacral spine. Patients were followed up for 6 months and the nature of the pain was assessed post-operatively using the Japanese orthopedic association system scoring for low back pain. It ranges from 0-15. Score 0 is the worst pain and 15 is normal.</p><p><strong>Results: </strong>The mean Japanese association score for low back pain was 7.2 before the surgical procedure and it was 12 after the procedure and the difference was found to be statistically significant. Among the postoperative complications, 4 patients developed failback syndrome and 1 patient had implant failure.</p><p><strong>Conclusios</strong><strong>: </strong>Relief of radicular pain was associated with subjective satisfaction with the surgery among our study population, as evidenced by the decrease in radicular pain and the subjective satisfaction with the operation.</p>
Sujeitos indígenas: estigmatizados pelas instituições
Authors: Melo Sílvia Mara de 
Published: April 2021

Este artigo corresponde a um dos frutos do projeto de pesquisa institucional intitulado “As representações midiáticas do sujeito índio sobrevivente no Estado do Mato Grosso do Sul, MS”, desenvolvido por mim na Universidade Federal da Grande Dourados, UFGD, e visa trazer à tona os estigmas e estereótipos veiculados em enunciados da comunidade acadêmica em relação a estudantes indígenas. Como corpus de pesquisa apresentamos tanto respostas de questionário aplicado a alunos indígenas e matérias jornalística do jornal O Progresso, mídia local. Para sustentar nossas análises, buscamos dialogar com autores do campo dos Estudos Culturais e com uma obra de Michel Foucault. A pergunta de pesquisa que norteou nossas análises foi: Como estigmas e estereótipos se constituem nas relações acadêmicas? Como resultado dessa investigação chegamos à conclusão de que as relações no âmbito educacional estão permeadas de preconceito e que as diferenças culturais promovem uma identidade deteriorada.Palavras-chave: Sujeito Indígena. Estigma. Instituição Educacional
Assessment of an antitumor effect of 2-(6,8-dimethyl-5-nitro-4-chloroquinoline-2-yl)-5,6,7-trichloro-1,3-tropolone in A-549 tumor cell subcutaneous xenografts
Authors: Lukbanova E. A. , Zaikina E. V. , Sayapin Yu. A. , Gusakov E. A. , Filippova S. Yu. , Zlatnik E. Yu. , Volkova A. V. , Kurbanova L. Z. , Khodakova D. V. , Kaymakchi D. O. , Lazutin Ju. N. , Shevchenko A. N. , Pandova O. V. 
Published: April 2021

Rationale: Chemotherapy is one of the lung cancer treatment methods. The search for new substances with antitumor effect against malignant lung neoplasms is relevant because of low efficacy and side effects of cytotoxic agents. A promising substance class with various biological activities, including antitumor, includes alkaloids of the tropolone family, such as heptamerous non-benzoid aromatic compounds. 2-(6,8-dimethyl-5-nitro-4-chloroquinoline-2-yl)- 5,6,7-trichloro-1,3-tropolone has been synthesized in Institute of Physical and Organic Chemistry; it is a new compound belonging to 2-quinoline-2-yl derivatives of 1,3-tropolone.Aim: To assess the antitumor effect of 2-(6,8-dimethyl-5-nitro-4-chloroquinoline-2-yl)- 5,6,7-trichloro-1,3-tropolone on subcutaneous xenografts of A-549 lung tumor cells in immunodeficient Balb/c Nude mice.Materials and methods: The study included 50 immunodeficient Balb/c Nude mice divided into 4 experimental groups depending on the dosage of the study substance (0.0055, 0.055, 0.55, and 2.75 mg/g); group 5 was the control group. A-549 cells of lung cancer were used as a xenograft. The antitumor effect of tropolone was evaluated by the inhibition of tumor growth and the index of tumor growth. The experiment lasted for 36 days starting from the first administration of the substances.Results: The mean tumor volumes on day 36 of the experiment in the control group and four experimental groups were 2729.5; 2150.8; 1746.4; 952.3 and 678.9 mm3 , respectively. The indices of tumor growth in groups 1, 2, 3 and 4 were significantly lower than in group 5 (control) starting from days 24, 21, 21 and 15, respectively, and till the end of the experiment. Maximal differences between groups 4 and 5 were observed at days 33 and 36 (by 3.7, p=0.01 and 4.1, p=0.003 times, respectively).Discussion: The anti-tumor effect of 2-(6,8-dimethyl-5-nitro-4-chloroquinoline-2-yl)- 5,6,7-trichloro-1,3-tropolone demonstrated in the study could be related to various mechanisms. For example, numerous studies have shown that its related compound hinokitiol exerts a cytotoxic effect associated with cessation of the cell cycle, apoptosis induction, DNA damage, and autophagic death of tumor cells.Conclusion: The study demonstrated significant differences in xenograft volumes in all experimental groups, compared to the control group. In mice, 2.75 mg/g bodyweight was the most effective dosage of the studied compound leading to a slow decrease in tumor growth rates and a decrease in the volumes of subcutaneous xenografts.