bluuebunny/crossref_metadata_2025_split huggingface dataset:
The effect of an orally-dosed Caralluma Fimbriata extract on appetite control and body composition in overweight adults
Authors: Rao Amanda , Briskey David , dos Reis Carla , Mallard Alistair R. 
Published: March 2021

AbstractTo examine the effect of a Caralluma Fimbriata extract (CFE) on biomarkers of satiety and body composition in overweight adults. A double-blind, randomised, placebo controlled trial to examine the effect of a Caralluma Fimbriata extract (CFE) on biomarkers of satiety and body composition in overweight adults. Eighty-three men and women aged between 20 and 50 years of age completed 16 weeks of daily supplementation with either CFE or placebo. Plasma cardiometabolic (lipid profile, glucose, insulin) and satiety (ghrelin, leptin, neuropeptideY) biomarkers, body composition, diet history and gastrointenstinal function were assessed at baseline, weeks 4, 8, 12 and 16. Subjects in the CFE and placebo groups were well matched and predominatly female 93% and 87.5%, with a mean age of 40.9 ± 6.7 and 39.5 ± 7.5 years and body mass index (BMI) of 30.0 ± 3.1 and 30.2 ± 2.9 kg/m2 respectively. There was a significant difference in plasma leptin concentration change between groups at week 16 (p = 0.04), with the placebo group increasing concentration (2.27 ± 4.80 ng/mL) while the CFE group (0.05 ± 4.69 ng/mL) remained the same. At week 16, the CFE group had significantly reduced their calorie intake from baseline compared to the placebo group (245 cal vs 15.8 cal respectively p < 0.01). The CFE group also had a significant reduction in waist circumference of 2.7 cm compared to an increase of 0.3 cm in the placebo group (p = 0.02). A weight increase from baseline was seen in the placebo group that was not observed in the CFE group (1.33 kg weight gain vs 0.37 kg weight loss respectively; p = 0.03). The placebo group also had a significant increase in fat mass, android fat mass, BMI and leptin compared to the CFE group (p = 0.04, 0.02, < 0.01 respectively). CFE was effective at maintaining bodyweight during a non-calorie controlled diet compared to a placebo. The mechanism responsible for this action is requiring further research and could be due to an increase in satiety receptor sensitivity.
CRISPY-BRED and CRISPY-BRIP: efficient bacteriophage engineering
Authors: Wetzel Katherine S. , Guerrero-Bustamante Carlos A. , Dedrick Rebekah M. , Ko Ching-Chung , Freeman Krista G. , Aull Haley G. , Divens Ashley M. , Rock Jeremy M. , Zack Kira M. , Hatfull Graham F. 
Published: March 2021

AbstractGenome engineering of bacteriophages provides opportunities for precise genetic dissection and for numerous phage applications including therapy. However, few methods are available for facile construction of unmarked precise deletions, insertions, gene replacements and point mutations in bacteriophages for most bacterial hosts. Here we describe CRISPY-BRED and CRISPY-BRIP, methods for efficient and precise engineering of phages in Mycobacterium species, with applicability to phages of a variety of other hosts. This recombineering approach uses phage-derived recombination proteins and Streptococcus thermophilus CRISPR-Cas9.
Comparative in silico genome analysis of Clostridium perfringens unravels stable phylogroups with different genome characteristics and pathogenic potential
Authors: Abdel-Glil Mostafa Y. , Thomas Prasad , Linde Jörg , Busch Anne , Wieler Lothar H. , Neubauer Heinrich , Seyboldt Christian 
Published: March 2021

AbstractClostridium perfringens causes a plethora of devastating infections, with toxin production being the underlying mechanism of pathogenicity in various hosts. Genomic analyses of 206 public-available C. perfringens strains´ sequence data identified a substantial degree of genomic variability in respect to episome content, chromosome size and mobile elements. However, the position and order of the local collinear blocks on the chromosome showed a considerable degree of preservation. The strains were divided into five stable phylogroups (I–V). Phylogroup I contained human food poisoning strains with chromosomal enterotoxin (cpe) and a Darmbrand strain characterized by a high frequency of mobile elements, a relatively small genome size and a marked loss of chromosomal genes, including loss of genes encoding virulence traits. These features might correspond to the adaptation of these strains to a particular habitat, causing human foodborne illnesses. This contrasts strains that belong to phylogroup II where the genome size points to the acquisition of genetic material. Most strains of phylogroup II have been isolated from enteric lesions in horses and dogs. Phylogroups III, IV and V are heterogeneous groups containing a variety of different strains, with phylogroup III being the most abundant (65.5%). In conclusion, C. perfringens displays five stable phylogroups reflecting different disease involvements, prompting further studies on the evolution of this highly important pathogen.
Fast physical repetitive patterns generation for masking in time-delay reservoir computing
Authors: Argyris Apostolos , Schwind Janek , Fischer Ingo 
Published: March 2021

AbstractAlbeit the conceptual simplicity of hardware reservoir computing, the various implementation schemes that have been proposed so far still face versatile challenges. The conceptually simplest implementation uses a time delay approach, where one replaces the ensemble of nonlinear nodes with a unique nonlinear node connected to a delayed feedback loop. This simplification comes at a price in other parts of the implementation; repetitive temporal masking sequences are required to map the input information onto the diverse states of the time delay reservoir. These sequences are commonly introduced by arbitrary waveform generators which is an expensive approach when exploring ultra-fast processing speeds. Here we propose the physical generation of clock-free, sub-nanosecond repetitive patterns, with increased intra-pattern diversity and their use as masking sequences. To that end, we investigate numerically a semiconductor laser with a short optical feedback cavity, a well-studied dynamical system that provides a wide diversity of emitted signals. We focus on those operating conditions that lead to a periodic signal generation, with multiple harmonic frequency tones and sub-nanosecond limit cycle dynamics. By tuning the strength of the different frequency tones in the microwave domain, we access a variety of repetitive patterns and sample them in order to obtain the desired masking sequences. Eventually, we apply them in a time delay reservoir computing approach and test them in a nonlinear time-series prediction task. In a performance comparison with masking sequences that originate from random values, we find that only minor compromises are made while significantly reducing the instrumentation requirements of the time delay reservoir computing system.
The effect of a smartphone-based pain management application on pain intensity and quality of life in adolescents with chronic pain
Authors: Shaygan Maryam , Jaberi Azita 
Published: March 2021

AbstractThe development, implementation, and qualitative evaluation of smartphone-based pain management applications may provide an opportunity for more optimal management of pediatric pain in the homesetting. The present mixed-method study was conducted to assess a smartphone-based pain management application regarding the feasibility, adherence, participant satisfaction, and effectiveness on pain intensity and quality of life in adolescents with chronic pain. The study was carried out in the quantitative and qualitative stages using a mixed-method approach. The quantitative stage included 128 adolescents who met the ICD-11 criteria of chronic pain. After random allocation, adolescents allocated to the intervention group received a pain management program through a smartphone-based application. No education was given to the adolescents in the control group. The adolescents were assessed regarding pain intensity and different dimensions of quality of life at pre-intervention, post-intervention, and three-month follow-up. The findings in the quantitative stage were explained by qualitative interviews. The findings of the quantitative stage showed significant improvements in the pain intensity, emotional, social, and school functioning but not in the physical functioning of the adolescents. A high level of adherence (78.12%) and satisfaction (Mean = 26.45, SD = 6.45) with thes martphone-based pain management program was found. Based on the qualitative interviews, adolescents’ pain management strategies can be classified in three main categories: physical management, psychological management, and interpersonal resources. The results confirm the positive effect of a smartphone-based pain management program on the pain intensity and different dimensions of quality of life of adolescents with chronic pain. Within the context of chronic pain management, a mobile application incorporating both the psychological and physical management of pain may help adolescents with chronic pain to reduce the negative impacts of pain on their life.
Comparison between two devices in the detection of corneal thickness changes after cataract surgery
Authors: De Bernardo Maddalena , Vitiello Livio , Abbinante Giulia , De Pascale Ilaria , Capasso Luigi , Marotta Giuseppe , Rosa Nicola 
Published: March 2021

AbstractThis study compared corneal thickness (CT) changes obtained with specular microscopy (SM) and a rotating Scheimpflug camera (RSC) after conventional phacoemulsification surgery (PS). One hundred sixty six eyes of 83 patients were analyzed before and one month after PS. One eye underwent PS, while the fellow phakic one was used as control. CT was measured with SM at the center of the cornea and with RSC at the pupil center, at the corneal apex and at the thinnest point. In the operated eye, SM showed a larger CT mean increase than those one detected at the three different measurements’ points evaluated by RSC. Inversely, in the fellow phakic eye, SM showed a greater CT mean decrease than those one registered by RSC at its three measurement’s points. Thus, one month after surgery, even if cornea appears clear at the slit-lamp, a significant thickness increase is still present. This is even more evident if the slight decrease of the fellow phakic eye is considered. The differences between the two devices are probably related to the different measured areas.
Phagocytosis of microparticles increases responsiveness of macrophage-like cell lines U937 and THP-1 to bacterial lipopolysaccharide and lipopeptide
Authors: Ueno Takayuki , Yamamoto Yumi , Kawasaki Kiyoshi 
Published: March 2021

AbstractFollowing bacterial infection, macrophages produce pro-inflammatory cytokines in response to bacterial cell components, including lipopolysaccharide (LPS) and lipopeptide, and simultaneously phagocytize and digest the invading bacteria. To study the effects of phagocytosis on pro-inflammatory responses, we determined if phagocytosis of polystyrene latex beads with ~ 1 µm diameter increases pro-inflammatory cytokine expression by human macrophage-like U937 and THP-1 cells stimulated with LPS. Treating macrophage-like cells with beads coated with IgG to facilitate Fcγ receptor-mediated phagocytosis increased LPS-induced expression of pro-inflammatory cytokines, including tumor necrosis factor-alpha, interleukin-1 beta, and interleukin-6. Treatment with beads coated with poly-l-lysine to facilitate Fcγ receptor–independent phagocytosis also increased LPS-induced cytokine expression. Our results indicate that LPS-induced pro-inflammatory responses are enhanced by bead phagocytosis regardless of the uptake mechanism. Additionally, phagocytosis enhanced LPS-induced NF-κB activation, suggesting that Toll-like receptor (TLR) 4 signaling is enhanced by phagocytosis. Furthermore, bead phagocytosis enhanced pro-inflammatory responses in U937 cells stimulated with lipopeptide, a ligand for the TLR2/TLR6 heterodimeric receptor. In conclusion, microparticle phagocytosis by macrophage-like U937 and THP-1 cells enhances the innate immune response induced by bacterial components.
Identifying the major lactate transporter of Toxoplasma gondii tachyzoites
Authors: Zeng Joy M. , Hapuarachchi Sanduni V. , Shafik Sarah H. , Martin Rowena E. , Kirk Kiaran , van Dooren Giel G. , Lehane Adele M. 
Published: March 2021

AbstractToxoplasma gondii and Plasmodium falciparum parasites both extrude l-lactate, a byproduct of glycolysis. The P. falciparum Formate Nitrite Transporter, PfFNT, mediates l-lactate transport across the plasma membrane of P. falciparum parasites and has been validated as a drug target. The T. gondii genome encodes three FNTs that have been shown to transport l-lactate, and which are proposed to be the targets of several inhibitors of T. gondii proliferation. Here, we show that each of the TgFNTs localize to the T. gondii plasma membrane and are capable of transporting l-lactate across it, with TgFNT1 making the primary contribution to l-lactate transport during the disease-causing lytic cycle of the parasite. We use the Xenopus oocyte expression system to provide direct measurements of l-lactate transport via TgFNT1. We undertake a genetic analysis of the importance of the tgfnt genes for parasite proliferation, and demonstrate that all three tgfnt genes can be disrupted individually and together without affecting the lytic cycle under in vitro culture conditions. Together, our experiments identify the major lactate transporter in the disease causing stage of T. gondii, and reveal that this transporter is not required for parasite proliferation, indicating that TgFNTs are unlikely to be targets for anti-Toxoplasma drugs.
Assessment of the relationship of serum liver enzymes activity with general and abdominal obesity in an urban Bangladeshi population
Authors: Ali Nurshad , Sumon Abu Hasan , Fariha Khandaker Atkia , Asaduzzaman Md , Kathak Rahanuma Raihanu , Molla Noyan Hossain , Mou Ananya Dutta , Barman Zitu , Hasan Mahmudul , Miah Rakib , Islam Farjana 
Published: March 2021

AbstractObesity is a global health concern because of its increasing trend both in developed and developing countries. A limited number of studies have evaluated the association of liver enzymes with both general and abdominal obesity in the general population; data for the Bangladeshi population are not available yet. This study aimed to assess the relationship of serum liver enzymes activity with both general and abdominal obesity in Bangladeshi adults. In total, 540 blood samples were obtained from the participants (388 males and 152 females) and analyzed for serum levels of ALT, AST, GGT, and ALP using standard methods. General obesity was defined as body mass index (BMI) ≥ 27.5 kg/m2and abdominal obesity was defined as waist circumference (WC) ≥ 90 cm in males and ≥ 80 cm in females. The relationship between liver enzymes and obesity was evaluated by multivariate logistic regression models. Overall, 58% of participants in the general obesity group and 55% of the participants in the abdominal obesity group had at least one or more elevated levels of liver enzymes. The prevalence of elevated liver enzymes was significantly higher in the obesity group compared to the normal BMI and WC groups (p < 0.05 for all cases). The mean level of serum ALT, AST and GGT were significantly higher in the obesity group than the normal BMI group (p < 0.05). In the WC groups, mean AST and GGT were significantly higher in the obesity group compared to the normal group (p < 0.05). In regression analysis, serum levels of ALT showed an independent and significant association with general obesity, whereas, serum GGT showed a significant association with both general and abdominal obesity. In conclusion, a high prevalence of elevated liver enzymes was observed among participants included in the present study. Of the four enzymes, serum GGT was independently associated with both general and abdominal obesity. Further studies are required to understand the complex relationship between liver enzymes and obesity in the general population.
Evidence for influx of Atlantic water masses to the Labrador Sea during the Last Glacial Maximum
Authors: Seidenkrantz Marit-Solveig , Kuijpers Antoon , Aagaard-Sørensen Steffen , Lindgreen Holger , Olsen Jesper , Pearce Christof 
Published: March 2021

AbstractThe Last Glacial Maximum (LGM, 23–19,000 year BP) designates a period of extensive glacial extent and very cold conditions on the Northern Hemisphere. The strength of ocean circulation during this period has been highly debated. Based on investigations of two marine sediment cores from the Davis Strait (1033 m water depth) and the northern Labrador Sea (2381 m), we demonstrate a significant influx of Atlantic-sourced water at both subsurface and intermediate depths during the LGM. Although surface-water conditions were cold and sea-ice loaded, the lower strata of the (proto) West Greenland Current carried a significant Atlantic (Irminger Sea-derived) Water signal, while at the deeper site the sea floor was swept by a water mass comparable with present Northeast Atlantic Deep Water. The persistent influx of these Atlantic-sourced waters entrained by boundary currents off SW Greenland demonstrates an active Atlantic Meridional Overturning Circulation during the LGM. Immediately after the LGM, deglaciation was characterized by a prominent deep-water ventilation event and potentially Labrador Sea Water formation, presumably related to brine formation and/or hyperpycnal meltwater flows. This was followed by a major re-arrangement of deep-water masses most likely linked to increased overflow at the Greenland-Scotland Ridge after ca 15 kyr BP.
Oncolytic peptides DTT-205 and DTT-304 induce complete regression and protective immune response in experimental murine colorectal cancer
Authors: Fleten Karianne Giller , Eksteen J. Johannes , Mauseth Brynjar , Camilio Ketil André , Vasskog Terje , Sveinbjørnsson Baldur , Rekdal Øystein , Mælandsmo Gunhild M. , Flatmark Kjersti 
Published: March 2021

AbstractOncolytic peptides represent a novel, promising cancer treatment strategy with activity in a broad spectrum of cancer entities, including colorectal cancer (CRC). Cancer cells are killed by immunogenic cell death, causing long-lasting anticancer immune responses, a feature of particular interest in non-immunogenic CRC. Oncolytic peptides DTT-205 and DTT-304 were administered by intratumoral injection in subcutaneous tumors established from murine CRC cell lines CT26 and MC38, and complete regression was obtained in the majority of animals. When cured animals were rechallenged by splenic injection of tumor cells, 1/23 animals developed liver metastases, compared to 19/22 naïve animals. Treatment with both peptides was well tolerated, but monitoring post-injection hemodynamic parameters in rats, less extensive changes were observed with DTT-205 than DTT-304, favoring DTT-205 for future drug development. DTT-205 was subsequently shown to have strong in vitro activity in a panel of 33 cancer cell lines. In conclusion, both peptides exerted a strong inhibitory effect in two immunocompetent CRC models and induced a systemic effect preventing development of liver metastases upon splenic rechallenge. If a similar effect could be obtained in humans, these drugs would be of particular interest for combinatory treatment with immune checkpoint inhibitors in metastatic CRC.
Genetic association of ERAP1 and ERAP2 with eclampsia and preeclampsia in northeastern Brazilian women
Authors: Ferreira Leonardo Capistrano , Gomes Carlos Eduardo Maia , Duggal Priya , De Paula Holanda Ingrid , de Lima Amanda Samara , do Nascimento Paulo Ricardo Porfírio , Jeronimo Selma Maria Bezerra 
Published: March 2021

AbstractThe clinical spectrum of hypertensive disorders of pregnancy (HDP) is determined by the interplay between environmental and genetic factors, most of which remains unknown. ERAP1, ERAP2 and LNPEP genes code for multifunctional aminopeptidases involved with antigen processing and degradation of small peptides such as angiotensin II (Ang II), vasopressin and oxytocin. We aimed to test for associations between genetic variants in aminopeptidases and HDP. A total of 1282 pregnant women (normotensive controls, n = 693; preeclampsia, n = 342; chronic hypertension with superimposed preeclampsia, n = 61; eclampsia, n = 74; and HELLP syndrome, n = 112) were genotyped for variants in LNPEP (rs27300, rs38034, rs2303138), ERAP1 (rs27044, rs30187) and ERAP2 (rs2549796 rs2927609 rs11135484). We also evaluated the effect of ERAP1 rs30187 on plasma Ang II levels in an additional cohort of 65 pregnant women. The genotype C/C, in ERAP1 rs30187 variant (c.1583 T > C, p.Lys528Arg), was associated with increased risk of eclampsia (OR = 1.85, p = 0.019) whereas ERAP2 haplotype rs2549796(C)–rs2927609(C)–rs11135484(G) was associated with preeclampsia (OR = 1.96, corrected p-value = 0.01). Ang II plasma levels did not differ across rs30187 genotypic groups (p = 0.895). In conclusion, ERAP1 gene is associated with eclampsia whereas ERAP2 is associated with preeclampsia, although the mechanism by which genetic variants in ERAPs influence the risk of preeclampsia and eclampsia remain to be elucidated.
The effect of polytetrafluoroethylene particle size on the properties of biodegradable poly(butylene succinate)-based composites
Authors: Chen Shudong , Peng Xiangfang , Geng Lihong , Wang Hankun , Lin Jialin , Chen Binyi , Huang An 
Published: March 2021

AbstractPoly(butylene succinate) (PBS)/polytetrafluoroethylene (PTFE) composites, including three types of PTFE powders, were prepared by melt blending using a HAAKE torque rheometer. Microcellular foams were successfully fabricated by batch foaming with supercritical fluids (scCO2). The effects of PTFE powder type on crystallization, rheological properties and foaming behavior were studied. PTFE L-5 and PTFE JH-220 powders showed good dispersion in the PBS matrix, and PTFE FA-500 powder underwent fibrillation during the melt blending process. All three PTFE powders gradually increased the crystallization temperature of PBS from 78.2 to 91.8 ℃ and the crystallinity from 45.6 to 61.7% without apparent changes in the crystal structure. Rheological results revealed that PBS/PTFE composites had a higher storage modulus, loss modulus, and complex viscosity than those of pure PBS. In particular, the complex viscosity of the PBS/P500 composite increased by an order of magnitude in the low-frequency region. The foamed structure of PBS was obviously improved by adding PTFE powder, and the effect of fibrillated PTFE FA-500 was the most remarkable, with a pore mean diameter of 5.46 μm and a pore density of 1.86 × 109 cells/cm3 (neat PBS foam: 32.49 μm and 1.95 × 107 cells/cm3). Moreover, PBS/P500 foam always guarantees hydrophobicity.