bluuebunny/crossref_metadata_2025_split huggingface dataset:
Salvage Treatment of Acute Respiratory Failure after Transcomposite Myocutaneous Flaps Transplantation for Chronic Empyema Combined with Chest Wall Sinus: A Case Report
Authors: Wang Lei , He Zhongliang 
Published: July 2021

Abstract
BackgroundChronic empyema with chest wall sinus is a complex and refractory disease caused by multiple factors.It may be combined with bronchopleural fistula, residual bone and other necrotic tissue,causing local infection difficult to control, and the disease is a vicious circle.Case presentationThis paper reports a 62-year-old male patient who underwent right pneumonectomy for squamous cell carcinoma of the lung 11 years ago and began to develop empyema with purulent sinus in the anterior chest wall 3 years ago. Therefore, he was admitted to our medical center for further treatment. Chest computed tomography (CT) showed the right pleural effusion with the chest wall rupture sinus. According to his clinical symptoms and imaging examination, he was diagnosed as chronic empyema with chest wall sinus.Due to the large empyema cavity,the patient should be treated with free vastus lateralis musculocutaneous flap combined with pedicled pectoralis major muscle flap transplantation.After the operation, acute respiratory failure occurred due to left lung aspiration pneumonia.ConclusionsAfter a series of treatment measures such as tracheal intubation, tracheotomy, anti-infection, maintenance of circulatory stability, and rehabilitation training, he was finally rescued and cured.Follow-up after discharge showed that the tissue flap survived and empyema was eliminated.
Acceptability and Safety of Thermal Ablation to Prevent Cervical Cancer in Sub-Saharan Africa
Authors: Metaxas Tania , Kenfack Bruno , Sormani Jessica , Tincho Eveline , Makajio Sophie Lemoupa , Wisniak Ania , Vassilakos Pierre , Petignat Patrick 
Published: July 2021

Abstract
Background - The World Health Organization recommends thermal ablation as an alternative to cryotherapy to treat women with precancerous lesions in low-resource settings. However, limited data are available on women’s experience and adverse events (AEs) of the procedure in the context of Sub-Saharan Africa. The objective of this study was to evaluate the acceptability and safety of thermal ablation in women screened positive for precancerous cervical lesions.Methods - Asymptomatic women aged 30-49 years old living in the Dschang Health District were invited to participate in a cervical cancer screening campaign termed “3T-Approach" (for Test-Triage and Treat). Recruited women were asked to perform HPV self-sampling followed by triage with visual assessment and treatment with thermal ablation if required. After treatment and 4-6 weeks later, interviews were conducted to assess women’s experience on anxiety, discomfort, and pain during thermal ablation. AEs were recorded on pre-defined electronic forms 4-6 weeks after treatment to assess the procedure’s safety.Results - Between September 2018 and January 2020, 399 HPV-positive women (18.7% of women screened) were recruited, 236 (59.1%) had a positive visual assessment, 234 were treated by thermal ablation and 198 (84.6%) received therapy in the same visit. Treatment was not considered as painful (score ≤4/10) by 209 (90.9%) patients while 5 (2.5%) reported high pain (score 8-10/10). During post-treatment interviews 4-6 weeks later, most reported AEs were graded mild or moderate (grade I-II). The most frequent symptoms reported as mild AEs (grade 1-2) were vaginal watery discharge (75.5%), vaginal bloody-stained discharge (21.5%) and malodourous discharge (14.5%). None of the participants experienced serious AEs (grade 3-4) or AEs requiring admission to hospital or emergency consultation. The vast majority of women (99.6%) would agree to repeat the procedure if necessary and (99.6%) would recommend it to friends or family. Conclusion - Thermal ablation is widely accepted by women and appears as a safe procedure. It may contribute to improving the link between screening and treatment in a single visit and to optimizing cervical cancer control in low-resource settings.Trial registrations: The study was registered on clinicaltrials.gov (NCT03757299) in November 2018 (28/11/2018).
Treatment Strategy of Oxaliplatin-induced Peripheral Neuropathy: A Retrospective, Nationwide Study
Authors: Yokoyama Satoshi , Nakagawa Chihiro , Hosomi Kouichi 
Published: July 2021

Abstract
PurposeChemotherapy-induced peripheral neuropathy (CIPN) is a common adverse events of cancer treatment; however, no drug is recommended for the prevention of CIPN. In Japan, several drugs such as Gosha-Jinki-Gan and duloxetine have been frequently administered for the treatment of CIPN. The aim of this study was to elucidate prescription patterns of drugs administered for the treatment of CIPN caused by oxaliplatin and the association between these drugs and the duration of oxaliplatin treatment.MethodsWe conducted a retrospective nationwide study using the JMDC administrative claims database (January 2005–June 2020). Patients newly treated with oxaliplatin were identified, and prescription patterns of CIPN medication including Gosha-Jinki-Gan, pregabalin, duloxetine, mecobalamin, and mirogabalin were investigated. The primary outcome was the duration of oxaliplatin treatment. Multivariable logistic regression analysis was performed to examine the association between CIPN medication and duration of oxaliplatin treatment.ResultsA total of 4,739 patients who newly received oxaliplatin were identified. Of these, 759 (16.0%) had received CIPN medication. Duloxetine was administered in 99 (2.1%) patients. Multivariable logistic regression analysis revealed that CIPN medication was significantly associated with the prolonged duration of oxaliplatin treatment (odds ratio: 2.35, [95% confidence interval: 1.99-2.77]).Conclusion﻿Real-world data demonstrated that the administration rate of CIPN medication was higher in patients who underwent oxaliplatin treatment for over 6 months. Increasing administration preference of duloxetine and conducting prospective studies to verify the causal relationship between CIPN medication and prolonged duration of oxaliplatin treatment are needed.
YKL-40 Enhanced the Permeability of HDMECs with Histamine Through Activating Akt and p38 Pathways
Authors: Zhou Peimei , Fu Lixin , Chen Tao , Wang Lin , Lu Yonghong , Wang Wenju , Zeng Shihua , Li Lin , Han Li 
Published: July 2021

Abstract
Background, YKL-40 is currently considered as an important marker of endothelial dysfunction. Chronic spontaneous urticaria (CSU) is a common vascular skin disease. The increased vascular permeability play an important role in the occurrence and pathogenesis of CSU.Objective, the aim of this study is to explore the role of YKL-40 on the permeability of HDMECs.Methods, in this study, the mRNA level of YKL-40 in human mast cell line (HMC-1) were detected by RT-PCR. The effects of YKL-40 on vascular permeability, VE-cadherin release, VE-cadherin disruption in human dermal microvascular endothelial cells (HDMECs) were investigated by transwell, ELISA or immunofluorescence. The phosphorylation of VE-cadherin, p38 and Akt, in histamine plus YKL-40 treated HDMECs were detected by Western Blot.Results, we found that YKL-40 significantly promoted the permeability changes and leaded to the released, disruption of VE-cadherin in HDMECs induced by histamine. Furthermore, YKL-40 also enhanced the Akt and p38 pathways. Conclusion, we suggest that YKL-40 may serve as pro-permeability cytokines, and play a role in the pathogenesis of CSU. This study will help to further elucidate the pathogenesis of CSU and provide a new target for the development of anti-histamine resistance drugs for CSU.
Contextual Factors Influencing Physicians’ Perception of Antibiotic Prescribing in Primary Care in Germany - A Prospective Observational Study
Authors: Queder Annika , Arnold Christine , Wensing Michel , Poß-Doering Regina 
Published: July 2021

Abstract
Background: Antimicrobial resistance is a worldwide challenge for health services and systems alike. To reduce the overuse of antibiotics, multifaceted interventions are often used to achieve sustainable effects. It can be assumed that these effects are influenced by contextual factors. Embedded in the cluster randomized trial ARena (Sustainable reduction of antibiotic-induced antimicrobial resistance), the aim of this present study was to identify contextual factors associated with practitioners’ perceptions of antibiotic prescribing in German primary health care. Methods: In a prospective observational study, data were generated in a three-wave survey study between January 2018 and July 2019. Analysis was performed using logistic regression models. The outcome of interest was the physician perceived impact of participating in the ARena project on decision-making regarding antibiotic prescribing, the independent variables of interest included individual characteristics, intervention arm allocation, primary care network (PCN) environment and characteristics of the medical practice.Results: 46.8% (n= 126) of participants indicated to have perceived an impact on their decision-making regarding antibiotic prescribing by participating in the ARena project. Bivariate logistic regression analyses indicated that work experience (odds ratio (OR) 1.05, 95% confidence interval (CI) 1.006–1.103), PCN environment (OR 2.06, 95% CI 1.256–3.363), structural conditions (OR 1.66, 95% CI 1.161–2.371), environment of existing processes (OR 1.46, 95% CI 1.011–2.094), and externally defined general conditions (OR 1.57, 95% CI 1.035–2.378) were associated with physicians’ perceived impact of participating in the ARena project on decision-making regarding antibiotic prescribing. In the multivariate logistic regression analysis, only work experience OR 1.05 (95% CI 1.001–1.104) continuously showed a significant influence.Conclusions: This study indicates that contextual factors at individual, practice, and system level influence physicians’ perceptions of antibiotic prescribing. Trial registration: ISRCTN, ISRCTN58150046 (registered 13.09.2017).
The Efficacy of PI3Kγ and EGFR Inhibitors on the Suppression of the Characteristics of Cancer Stem Cells
Authors: Xu Yanning , Afify Said M. , Du Juan , Liu Bingbing , Wang Qing , Li Hanbo , Liu Yixin , Fu Xiaoying , Zhu Zhengmao , Chen Ling , Seno Masaharu 
Published: July 2021

Abstract
Cancer stem cells (CSCs) are capable of continuous proliferation, self-renewal and are proposed to play significant roles in oncogenesis, tumor growth, metastasis and cancer recurrence. We have established a model of CSCs that was originally developed from mouse induced pluripotent stem cells (miPSCs) by proposing miPSCs to the conditioned medium (CM) of cancer derived cells, which is a mimic of carcinoma microenvironment. Further research found that not only PI3K-Akt but also EGFR signaling pathway was activated during converting miPSCs into CSCs. In this study, we tried to observe both of PI3Kγ inhibitor Eganelisib and EGFR inhibitor Gefitinib antitumor effects on the models of CSCs derived from miPSCs (miPS-CSC) in vitro and in vivo. As the results, targeting these two pathways exhibited significant inhibition of cell proliferation, self-renewal, migration and invasion abilities in vitro. Both Eganelisib and Gefitinib showed antitumor effects in vivo while Eganelisib displayed more significant therapeutic efficacy and less side effects than Gefitinib on all miPS-CSC models. Thus, these data suggest that the inhibitiors of PI3K and EGFR, especially PI3Kγ, might be a promising therapeutic strategy against CSCs defeating cancer in the near future
Stimulation of Phenolic Compounds Accumulation and Antioxidant Activity in In Vitro Culture of Salvia Tebesana Bunge in Response to Nano-TiO2 and Methyl Jasmonate Elicitors
Authors: Shoja Amin allah , Cheniany Monireh - , Ganjeali Ali 
Published: July 2021

Abstract
In the present study, we first attempted to achieve an efficient procedure for optimizing callogenesis from apical meristem and leaf explants of S. tebesana on MS media containing different concentrations of BAP alone and in combination with 2,4-D. Then, the inducing effect of nano-TiO2 (10, 60, and 120 mg L− 1) and methyl jasmonate (50, 100, and 200 µM), as abiotic elicitors were studied on the enhancement of phenolic compounds, rosmarinic acid, and some individual flavonoids as well as antioxidant capacity of callus extracts. According to the results, the highest callogenesis rate (100 and 93.33, respectively) and DW (0.55 ± 0.03 and 0.36 ± 0.02 g, respectively) per responsive explant were achieved from apical meristem on MS media containing "BAP 1 + 2,4-D 1" mg L− 1 and from leaf explant on the medium supplemented with "BAP 0.5 + 2,4-D 1" mg L− 1. The elicitation with 10 and 60 mg L− 1 nano-TiO2 (respectively for apical meristem and leaf), and 50µM MeJa could significantly promote the production of predominant phenolic derivatives in S. tebesana calli, where the highest content of total phenolics, O-diphenols, phenolic acid, flavonoid, flavone and flavonol, proanthocyanidin was recorded. Additionally, in increasing the amount of rosmarinic acid of callus, nano-TiO2 treatment was more effective than the elicitation with MeJa. Also, the highest content of Apigenin (0.33 ± 0.02 µg g− 1 DW) was detected after MeJa-elicitation (50µM), while the maximum level of Quercetin (2.61 ± 0.09 µg g− 1 DW) and Rutin (13.79 ± 08 µg g− 1 DW) were obtained after exposure to 60 mg L− 1 nano-TiO2, both from leaf-derived calli. While a significant positive correlation was recorded between antioxidant assays (DPPH and FRAP) and phenolic derivatives of treated calli; a very strong correlation occurred between the content of rosmarinic acid of apical meristem-derived calli and DPPH and FRAP values (r2 = -0.921 and r2 = -0.913, P < 0.01 respectively). Our results showed that the combination of in vitro culture and elicitation would be a good technique to successfully produce and enhance the content of pharmacologically valuable metabolites in S. tebesana.
Dual Electrochemical Sensing of Spiked Virus and SARS-CoV-2 Using Natural Bed-Receptor (MV-gal1)
Authors: ghazizadeh lham , Neshastehriz Ali , Firoozabadi Ali Dehghani , Saievar-Iranizad Esmail , Einali Samira 
Published: July 2021

Abstract
It has been necessary to use methods that can detect the specificity of a virus during virus screening. In this study, we use a dual platform to identify any spiked virus and specific SARS-CoV-2 antigen, sequentially. We introduce a natural bed-receptor surface as Microparticle Vesicle-Galactins1 (MV-gal1) with the ability of glycan binding to screen every spiked virus. MV are the native vesicles which may have the gal-1receptor. Gal-1 is the one of lectin receptor which can bind to glycan. After dropping the MV-gal1 on the SCPE/GNP, the sensor is turned on due to the increased electrochemical exchange with [Fe(CN)6] -3/-4 probe. Dropping the viral particles of SARS-CoV-2 cause to turn off the sensor with covering the sugar bond (early screening). Then, with the addition of Au /Antibody-SARS-CoV-2 on the MV-gal1@ SARS-CoV-2 Antigen, the sensor is turned on again due to the electrochemical amplifier of AuNP (specific detection). As a result, our sensor has the capacity of screening of every spike virus, and the specific detection of covid-19 (LOD: 4.57 × 102 copies/mL) by using the natural bed-receptor and a specific antibody in the solid biosensor, for the first time.
An Experimental Validation Study of Ferrofluid Evaporation
Authors: YU Wenjuan , Li Decai , Niu Sifang 
Published: July 2021

Abstract
Kerosene based ferrofluid was put into a test tube to evaporate under different conditions. The weight losses of samples were measured and the evaporation rates were calculated. The predictions of evaporation rates were made based on Bolotov’s model. It was found that the magnetic particles prevent the base fluid from evaporation and lower volume fraction leaded to higher evaporation rate. Bolotov’s model had a certain deviation but still well responsive to different variables. It was also found that the magnetic field made a difference to the evaporation rate.
An Unconstrained Face Recognition Method Based on Siamese Networks
Authors: CunLi Song , Ji Shouyong 
Published: July 2021

Abstract
It is aimed at the low accuracy and low efficiency of face recognition under unlimited conditions.In this paper, a Siamese neural Network model SN-LF (Siamese Network based on LBP and Frequency Feature perception) is designed based on the Local Binary Pattern (LBP) and the Frequency sensing model.Based on Siamese neural networks, the network adopts circular LBP algorithm and frequency feature perception to realize face recognition under unrestricted conditions.The LBP algorithm can eliminate the influence of light on the image and provide directional input to the network model at the same time.Frequency feature sensing divides the image features into low frequency features and high frequency features. The low frequency features are compressed in the Siamese neural network to increase the recognition efficiency of the network. At the same time, information is exchanged with the high frequency features, so that the target noise data can be eliminated while the feature data is retained.In this way, the recognition rate of the network is maintained, and the computing speed of the network is improved.Simulation experiments are carried out on standard face dataset CASIA-Webface and Yale-B, and compared with other network models. The experimental results show that the proposed SN-LF network structure can improve the recognition accuracy of the algorithm, and achieve a good recognition accuracy.
A Pythagorean Cubic Fuzzy Methodology Based on TOPSIS and TODIM Methods and its Application to Software Selection Problem
Authors: Seker Sukran , Kahraman Cengiz 
Published: July 2021

Abstract
Software selection process for many organizations is a challenging task to conduct their business activities and sustain competitiveness. This paper develops a new hybrid multi-criteria decision making (MCDM) method to select the most effiecient vendor-supplied software package which is used in all business activities for planning or designing, organizing, and supervising functions by operations management of a fuel oil company operated in Turkey. The proposed method is a hybridization of two well-known MCDM approaches, namely TODIM (an acronym in Portuguese for Interactive and Multi criteria Decision Making) and TOPSIS (technique for order preference by similarity to an ideal solution) using Pythagorean cubic fuzzy sets (PCFS) to manage uncertainty, subjectivity and bias of decision makers (DMs). To prove the efficiency and applicability of the proposed method, a real life application to select best software package for fuel oil company, is conducted. Finally, a sensitivity and comparison analyses are carried out to verify validity and stability of the results obtained by the proposed approach.
Multi-scale neural signatures of major depressive, anxiety, and stress-related disorders
Authors: Zhukovsky Peter , Wainberg Michael , Milic Milos , Tripathy Shreejoy , Mulsant Benoit , Felsky Daniel , Voineskos Aristotle 
Published: July 2021

Abstract
The extent of shared and distinct neural mechanisms underlying major depressive disorder (MDD), anxiety and stress-related disorders is still unclear. We compared the neural signatures of these disorders in 5,405 UK Biobank patients and 21,727 healthy controls. We found the greatest case-control differences in resting-state functional connectivity and cortical thickness in MDD, followed by anxiety and stress-related disorders. Neural signatures for MDD and anxiety disorders were highly concordant, namely in altered frontostriatal connectivity, whereas stress-related disorders showed a distinct pattern. Controlling for cross-disorder genetic risk somewhat increased the similarity between functional neural signatures of stress-related disorders and both MDD and anxiety disorders. Among cases and healthy controls, reduced within-network and increased between-network frontoparietal and default mode connectivity was associated with poorer cognitive performance (processing speed, attention, associative learning and fluid intelligence). These results provide evidence for distinct neural circuit function impairments in MDD and anxiety disorders compared to stress disorders; yet cognitive impairment appears unrelated to diagnosis and varies with circuit function.
Oral Health Among Children with Haematological Malignancies in Syria
Authors: Alhaffar MHD Bahaa Aldin , Mustafa Khattab , Alsabbagh Samira , Alhaffar Ola , Kakaje Ameer 
Published: July 2021

Abstract
Background:Since 2011, Syria has been facing a conflict that impacted all aspects of life. Haematological malignancies are mainly treated with chemotherapy which can further harm oral health. This study evaluates oral health of children with haematological malignancies and on chemotherapy in Syria.Methods: This is a cross-sectional study of (40) patients from the major paediatric hospital in Damascus. Decayed, Missing, Filled Teeth (DMFT) and Oral Assessment Guide (OAG) indices were used to evaluate oral health. Data about cancer was collected from medical records. Results: Patients aged between 3-14 years. Three types of paediatric cancer were included; acute lymphoblastic leukaemia (ALL) 52.2%, Hodgkin’s Lymphoma (HL) 27.5%, and Burkitt Lymphoma (BL) 20%. Average DMFT was (6.7 ± 3.2) and average OAG index was (10.8 ± 1.7). DMFT was significantly correlated with OAG (r=0.021). In patients undergoing chemotherapy, better DMFT and OAG indices were reported in males who had cancer for shorter periods, had higher frequency of toothbrushing, or/and were helped while toothbrushing. However, age and cancer type did not significantly affect DMFT and OAG.Conclusion: Children undergoing chemotherapy in Syria had worse oral health and pain compared to other children from the same age in similar studies. More attention should be given to these patients in order to improve their overall health and their quality of life especially during the ongoing conflict.
Chasing perfection: validation and polishing strategies for telomere-to-telomere genome assemblies
Authors: Rhie Arang , Cartney Ann Mc , Shafin Kishwar , Alonge Michael , Bzikadze Andrey , Formenti Giulio , Fungtammasan Arkarachai , Howe Kerstin , Jain Chirag , Koren Sergey , Logsdon Glennis , Miga Karen , Mikheenko Alla , Paten Benedict , Shumate Alaina , Soto Daniela , Sović Ivan , Wood Jonathan , Zook Justin , Phillippy Adam 
Published: July 2021

Abstract
Advances in long-read sequencing technologies and genome assembly methods have enabled the recent completion of the first Telomere-to-Telomere (T2T) human genome assembly, which resolves complex segmental duplications and large tandem repeats, including centromeric satellite arrays in a complete hydatidiform mole (CHM13). Though derived from highly accurate sequencing, evaluation revealed that the initial T2T draft assembly had evidence of small errors and structural misassemblies. To correct these errors, we designed a novel repeat-aware polishing strategy that made accurate assembly corrections in large repeats without overcorrection, ultimately fixing 51% of the existing errors and improving the assembly QV to 73.9. By comparing our results to standard automated polishing tools, we outline common polishing errors and offer practical suggestions for genome projects with limited resources. We also show how sequencing biases in both PacBio HiFi and Oxford Nanopore Technologies reads cause signature assembly errors that can be corrected with a diverse panel of sequencing technologies
Phenotypic Clusters On Computed Tomography Reflects Asthma Heterogeneity And Severity
Authors: Kim Sujeong , Choi Sanghun , Kim Taewoo , Jin Kwang Nam , Cho Sang-Heon , Lee Chang Hyun , Kang Hye-Ryun 
Published: July 2021

Abstract
The authors have requested that this preprint be removed from Research Square.