bluuebunny/crossref_metadata_2025_split huggingface dataset:
Assement of Anxiety and Factor Related Among Mizan Tepi University Freshman Students, South, Ethiopia 2021
Authors: wolde yetayale berhanu , Ayenalem Alem Eskeziya 
Published: December 2021

Abstract
Background

Anxiety can disturb a student's academic success and his future, including withdrawal from classes. The dropout rate was found to be twice as high as in the 1st grade compared to the 2nd or 3rd grade. It also causes people around the world to feel excessive anxiety, fear, lack of self-confidence, and embarrassment. One of the key reasons in the current study is the lack of data on the degree of mental illness, especially in higher education institutions. Objective: To assess the anxiety and factors of Mizan Tepi University students.
METHODS

From April 21th to May 21, 2020, an organization-based cross-sectional survey was conducted in the city of Mizan Tepi. The survey used a structured questionnaire and a self-administered questionnaire-ASQ tool. The stratified sampling method included 301 individuals in the study.Pre-test questionnaires that are also used for data collection. Data analysis was done using SPSS version 20.
Result

There are 320 freshmen students of these, 301 attended during the investigation. There are 162 (54%) male respondents and 138 (46%) female respondents in the study. The average student age and (SE) age are 21.6, (± 0.13) years old. The prevalence of anxiety was found to be 26.7% among first-year students. Compared with males (16.7%), female freshmen (40.6%) have a statistically higher incidence of anxiety. [AOR 95%CI=3.36 (1.88, 6.01)
Conclusion

his study shows that anxiety is a common mental problem among college students calling for public intervention to prevent more serious forms of anxiety. This finding indicates that women and older students have a high chance of suffering from anxiety.
Relative Sensitivity of Common Target Genes For The Detection of SARS-Cov-2 In Real Time-PCR
Authors: Valadan Reza , Golchin Soheila , Alizadeh-Navaei Reza , Haghshenas Mohammadreza , Zargari Mehryar , Mousavi Tahoora , Ghamati Mohammad 
Published: December 2021

Abstract
SARS-CoV-2(COVID-19) currently is the main cause of the severe acute respiratory disease and fatal outcomes in human beings worldwide. Several genes are used as targets for the detection of SARS-CoV-2, including the RDRP, N, and E genes. The present study aimed to determine the RDRP, N, and E genes expressions of SARS-CoV- 2 in clinical samples. For this purpose, 100 SARS-CoV-2 positive samples were collected from diagnostic laboratories of Mazandaran province, Iran. After RNA extraction, the real time RT-PCR assay was performed for differential gene expressions’ analysis of N, E, and RDRP. The CT values for N, RDRP, and E targets of 100 clinical samples for identifying SARS-CoV-2 were then evaluated using qRT-PCR. This result suggests N gene as a potential target for the detection of the SARS‐CoV‐2, since it was observed to be highly expressed in the nasopharyngeal or oropharynges of COVID-19 patients (P < 0.0001). Herein, we showed that SARS-CoV- 2 genes were differentially expressed in the host cells. Therefore, to reduce obtaining false negative results and to increase the sensitivity of the available diagnostic tests, the target genes should be carefully selected based on the most expressed genes in the cells.
Identification of a Potential Autophagy-Associated Therapeutic Target in Osteosarcoma using Bioinformatic Analyses
Authors: Feng Haijun , Wang Liping , Liu Jie , Wang Shengbao 
Published: December 2021

Abstract
Background: Osteosarcoma, primarily resulting from mesenchymal cell differentiation, is the most common primary malignancy of the bone in children and adolescents. Metastases to other sites, such as the lung, often occur in the early stages and progress rapidly. Autophagy functions as a tumor-suppressing mechanism in the early stages of oncogenesis, however, in later stages, autophagy may promote tumor growth, spread, and increase treatment resistance. Methods: In the present study, we aimed to screen new key autophagy-related biomarkers that may serve as therapeutic targets for osteosarcoma. The expression profile of the GSE42352 dataset, including 103 OS cell lines and 15 MSC lines from Gene Expression Omnibus (GEO), was analyzed to identify the differentially expressed genes. Results: WGCNA was used to construct the gene co-expression network in osteosarcoma, identify co-expression modules for protein interactions (PPI), and screen the key genes. A total of 757 differentially expressed genes were identified by WGCNA analysis including one key autophagy-related module. Conclusions: Further, we performed the PPI analysis for module genes and identified a key gene. We also theoretically and functionally validated its expression using the validation dataset GSE14359, and in vitro experiments, respectively.
In Different Gender Groups, What Is the Impact of the Fibular Notch on The Severity of High Ankle Sprain: A Retrospective Study of 240 Cases
Authors: Peng Xiaoyao , Su Fan , Tang Xiangyu , Yang Yuening , Chen Junyao , You Guixuan , Huang Lei , Wang Guoyou , Zhang Lei 
Published: December 2021

Abstract
Background: The function of the distal tibiofibular ligament on the ankle in the occurrence of high ankle sprain (HAS) has been widely studied. Then, in different genders, the effect of the anatomical morphology of fibular notch (FN) on HAS is unclear. Therefore, on the basis of excluding the anatomical differences caused by gender, we explore the impact of different types of FN on the severity of HAS.Methods: We selected 120 patients and further classified these 120 patients into four HAS groups according to FN depth with deep concave type FN ≥ four mm and shallow flat type FN < four mm. A further 120 normal individuals were served as a control group. FN morphological indicators, tibiofibular distance (TFD), and ankle mortise indexes were measured and compared between patients and control groups.Results: In males with shallow flat type, the Anterior tibiofibular distance (aTFD), Middle tibiofibular distance (mTFD), Posterior tibiofibular distance (pTFD), Front tibial width (FTiW), Middle tibial width (MTiW), Posterior tibial width (PTiW) and Depth of ankle mortise (DOAM) of HAS group were higher than those in normal group (P < 0.05). In males with deep concave type, the aTFD, mTFD and DOAM of patients were significantly higher (P < 0.05). Among females with shallow flat type, the aTFD, mTFD, pTFD, FTiW and MTiW in HAS group were greater than those in normal group (P < 0.05). Among the females with deep concave type, the mTFD and pTFD of patients were higher (P < 0.05).Conclusions: After analyzing the morphological indicators of FN, it is found that in both males and females, HAS patients have significant differences in TFD and certain ankle mortise indexes compared with normal people. But more importantly, the above abnormalities are often more common in HAS patients with shallow flat FN, indicating that shallow flat FN may be related to more serious distal tibiofibular ligament injury and ankle mortise widening, resulting in a worse prognosis.Level of evidence: Level III, retrospective comparative study.
Landscape Integrity Eclipses Local Effects of Floral Resource Availability on Bumble Bee (Bombus Spp.) Abundance in a Water-Limited Island Ecosystem
Authors: Simon Andrew , Starzomski Brian M. 
Published: December 2021

Abstract
ContextHabitat loss threatens to exacerbate climate change impacts on pollinator communities, particularly in Mediterranean-type ecosystems where late season floral resource availability is limited by seasonal drought. While gardens have been found to supplement floral resources in water-limited urban landscapes, less is known about the role of natural habitat diversity in sustaining late season floral resources in more intact landscapes. ObjectivesWe investigated the importance of habitat integrity and diversity for bumble bees in a water-limited ecosystem, observing bumble bee community response to seasonal drought across gradients of disturbance and soil moisture.MethodsWe applied hierarchical models to estimate the effects of local site conditions versus landscape scale estimates of matrix habitat on bumble bee abundance. Floral resources, soil moisture, and other environmental variables were sampled along randomly distributed belt transects. Geospatial estimates of matrix habitat were derived from terrestrial ecosystem data. Bumble bees were sampled with blue vane traps.ResultsIn the late season we found that modified wet areas supported more floral resources and bumble bee workers as compared to dry semi-natural environments. Wetlands also supported more late season floral resources and bumble bee workers, though the latter effect was not significant. Despite higher levels of late season floral resources in modified wet environments, modified matrix habitat was negatively associated, and natural matrix positively associated, with workers in June and late-flying queens in July and August. We also detected differences in bumble bee community composition in disturbed versus undisturbed environments.ConclusionsThough wet modified habitats sustained the highest levels of late season floral resource availability and worker abundances in our study, bumble bee diversity and abundance were limited primarily by the availability of natural matrix habitat at the landscape scale. The conservation of natural habitat integrity and diversity can help support critical nesting and foraging habitat, and should be prioritized in efforts to foster the resilience of pollinator communities.
Nouns and Verbs Identify Different Subtypes of MCI
Authors: Bao Zhiming , Cao Luwen , Han Kunmei , Li Lin , Hing Jia Wen , Ooi Vincent , Huang Nick , Feng Lei , Mahendran Rathi , Kua Ee Heok 
Published: December 2021

Abstract
It is well-documented that patients with semantic dementia and Alzheimer’s disease present with difficulty in lexical retrieval and reversal of the concreteness effect in nouns and verbs. Little is known about the lexical phenomena before the onset of symptoms. We anticipate that there are linguistic signs in the speech of people who suffer from mild cognitive impairment (MCI), the prodromal stage of dementia. Here, we report the results of a novel corpus-linguistic approach to the early detection of cognitive impairment. We recorded 40 hours of natural, unconstrained speech of 188 English-speaking Singaporeans; 90 are diagnosed with MCI (51 amnestic, 39 nonamnestic), and 98 are cognitively healthy. The recordings yield 327,470 words, which are tagged for parts of speech. We calculate the per-minute speech rates and concreteness scores of nouns and verbs, and of all tagged words, in our dataset. Our analysis shows that the two measures of nouns and verbs identify different subtypes of MCI. Compared with healthy controls, subjects with amnestic MCI produce fewer but more abstract nouns, whereas subjects with nonamnestic MCI produce fewer but more concrete verbs. Cognitive impairment is manifested in ordinary language before the presentation of clinical symptoms, and can be detected through non-invasive corpus-based analysis of natural speech.
Synergistic Antitumor Effect of Andrographolide and Cisplatin Through ROS-Mediated ER Stress and STAT3 Inhibition in Colon Cancer
Authors: Hong Huang , Cao Weilan , Wang Quanpeng , Liu Changbao , Huang Chongjie 
Published: December 2021

Abstract
Colon cancer is one of the most leading death-causing cancers in the world. Cisplatin has been widely used as the first-line treatment of cancer. However, its clinical application is limited by the side effects or acquired drug resistance. Hence, it is of vital clinical significance to develop novel agents that synergize with cisplatin and decrease its side effects. The aim of this study was to investigate whether Andrographolide (AP) synergistically potentiates the anti-tumor effect of cisplatin on colon cancer cells. Here, we found that AP synergizes with cisplatin in exerting anticancer activity in colon cancer cells. Further studies showed that AP potentiates cisplatin-induced endoplasmic reticulum stress and STAT3 inhibition through increasing intracellular ROS. Notably, pre-treatment of NAC, a ROS scavenger, reversed apoptosis induced by combined treatment of AP and cisplatin, while relieving the activation of endoplasmic reticulum stress as well as STAT3 inhibition. These findings indicated that ROS plays a pivotal role in mediating synergistic anticancer effects of AP and cisplatin on colon cancer cells. Overall, this study presents a potential new therapeutic strategy for the treatment of colon cancer.
The Efficacy and Mechanism of Positive Psychological Intervention on Well-Being for Colostomy Patients: A Randomized Controlled Trial
Authors: Jin Yanfei , Wang Yuqing , Ma Hongwen , Tian Xu , Honghong Wang 
Published: December 2021

Abstract
Aims
To evaluate the efficacy and mechanism of positive psychological intervention (PPI) on the psychological capital, psychological distress, and life satisfaction among colostomy patients.
Methods
Patients (n=120) with permanent stomas were recruited and randomly assigned into two groups. Patients in the experimental group (n=60) received standard care and PPI, whereas patients in the control group (n=60) only received standard care. The psychological capital, psychological distress, and life satisfaction were measured and compared between two groups before the intervention, the immediate post-intervention, and follow-up.
Results
All 120 patients completed the study. The hope, optimism, resilience, psychological distress, and life satisfaction score of the experimental group were significantly higher than those of the control group at T1 and T2 (P<0.05). Self-efficacy score of the experimental group had no significant difference at the two-time points after the intervention than the control group (P>0.05). Changes in hope and resilience which belong to psychological capital mediated the intervention’s efficacy on changes in PPI on life satisfaction (β = 0.265, P=0.005; β = 0.686, P=0.002).
Conclusions
PPI could effectively improve psychological capital, psychological distress, and life satisfaction among patients with stomas. Besides, our findings add novel support that increased hope and resilience are the active ingredients that promote intervention change.
Adverse Outcomes Following Free Tissue Transfer: A 3-year Experience From a Major Referral Centre
Authors: Nann Silas , Yip Jia Miin , Glanville Tyler , Marshall Nicholas 
Published: December 2021

Abstract
BackgroundFree tissue transfer encompasses a variety of techniques by which tissue is moved to another region of the body, with anastomosis of the divided artery and vein. Currently, success rates are reported at 91-99% [1], however, little is known regarding predictors for adverse outcomes.We aim at identifying predictors for negative outcomes following free flap surgery; and predict that elderly patients and patients with head and neck free flaps will have inferior outcomes due to comorbidity.MethodsThis is a retrospective case series. All free flap surgeries between 02/2018 to 02/2021 were identified using the electronic operation record system at Flinders Medical Centre. Chi squared hypothesis testing assessed patient factors and implications on outcome. Results67 patients of varying demographics were included in this study. The odds of wound infection was much higher in patients aged older than 65 (OR: 4.1 (95%CI 1.24-13.6, z-score: 2.31, p=0.017)). The odds of unplanned reoperation was also higher in this population (OR: 13.7 (95%CI 1.42-132.9, p=0.0053)). Free flap location was significant in determining whether patients would require a subsequent blood transfusion (p=0.0071). Head and neck patients did not experience a higher rate of adverse events.ConclusionPatients aged 65 and older are more likely to require treatment for infection and more often require reoperation because of flap related issues. Patients with limb free flaps are more likely to require transfusions. Head and neck patients did not have higher complication rates.
Strengthening Mental Abilities with Relational Training (SMART) in Multiple Sclerosis (MS): Study Protocol for a Feasibility Randomised Controlled Trial
Authors: Moghaddam Nima , Dawson David L , Evangelou Nikos , Turton James , Hawton Annie , Law Graham , Roche Bryan , Rowan Elise , Burge Rupert , Frost Alexandra C , Nair Roshan das 
Published: December 2021

Abstract
Background

Multiple Sclerosis (MS) is a chronic condition of the central nervous system, affecting around 1 in every 600 people in the UK, with 130 new diagnoses every week. Cognitive difficulties are common amongst people with MS, with up to 70% experiencing deficits in higher-level brain functions – such as planning and problem-solving, attention, and memory. Cognitive deficits make it difficult for people with MS to complete everyday tasks and limit their abilities to work, socialise, and live independently. There is a clear need – and recognised research priority – for treatments that can improve cognitive functioning in people with MS. The absence of effective cognitive interventions exacerbates burdens on the services accessed by people with MS – requiring these services to manage sequelae of untreated cognitive deficits, including reduced quality of life, greater disability and dependence, and poorer adherence to disease-modifying treatments. Our planned research will fill the evidence gap through developing – and examining the feasibility of trialling – a novel online cognitive rehabilitation programme for people with MS (SMART).
Methods

The primary objective of this study aims to conduct a feasibility study to inform development of a definitive trial of SMART for improving cognitive functioning in people with MS. Secondary objectives include accessing the acceptability to participants of the intervention, delivery format, inclusion/exclusion criteria, baselines and outcome measures, randomisation protocol, and the study procedures. It will further assess the framework for a cost-effectiveness analysis alongside a definitive trial; participant recruitment and retention rates, sample-size needed for a fully powered trial, and signal of efficacy.
Discussion

As a feasibility trial, outcomes are unlikely to immediately effect changes to NHS practice. However, this is a necessary step towards developing a definitive trial – and will give us a signal of efficacy, a prerequisite for progression to a definitive trial. If found to be clinically- and cost-effective, the latter trial could create a step-change in MS cognitive rehabilitation – improving service-delivery and optimising support with limited additional resources.
Trial Registration:

Registration ID: ClnicalTrials.gov: NCT04975685 – registered on July 23rd, 2021
Protocol version:
2.0, 25 November 2021
Genome-wide Analysis of CNVs in Three Populations of Tibetan Sheep using Whole-genome Resequencing
Authors: Hu Linyong , Li Qi , Zhang Liangzhi , Liu Hongjin , Xu Tianwei , Zhao Na , Han Xueping , Xu Shixiao , Zhao Xinquan , Zhang Cunfang 
Published: December 2021

Abstract
Background: Copy number variation (CNV), an important source of genomic structural variation, can disturb genetic structure, dosage, regulation and expression, and is associated with phenotypic diversity and adaptation to local environments in mammals. In the present study, 24 resequencing datasets were used to characterize CNVs in three ecotypic populations of Tibetan sheep and assess CNVs related to domestication and adaptations in the Qinghai-Tibetan Plateau.Results: A total of 87,832 CNV events accounting for 0.3% of the sheep genome were detected in three Tibetan sheep populations. After merging the overlapping CNVs, 2777 CNV regions (CNVRs) were obtained, among which 1098 CNVRs were shared by the three populations. The average length of these CNVRs was more than 3 kb, and duplication events were more frequent than deletions. Functional analysis showed that the shared CNVRs were significantly enriched in 56 GO terms and 18 KEGG pathways that were mainly concerned with ABC transporters, olfactory transduction and oxygen transport. Moreover, 188 CNVRs overlapped with 97 quantitative trait loci (QTLs), such as growth and carcass QTLs, immunoglobulin QTLs, milk yield QTLs and fecal counts QTLs. PCDH15, APP and GRID2 overlapped with body weight QTLs. Furthermore, Vst analysis at each CNVR showed that RUNX1, LOC101104348, LOC105604082 and PAG11 were highly divergent between HTS and VTS, and RUNX1 and LOC101111988 were significantly differentiated between VTS and OTS. Meaningfully, the duplication of RUNX1 may facilitate the hypoxia adaptation of OTS and HTS in QTP, which deserves further research in detail.Conclusions: In this study, we represented the genome-wide distribution characteristics of CNVs in Tibetan sheep and provided a valuable genetic variation resource, which will facilitate the elucidation of the genetic basis underlying the distinct phenotypic traits and local adaptation of Tibetan sheep.
Estimated 24 Hour Urinary Sodium and Potassium Excretion in a Population of Moroccan Adults
Authors: idrissi Mohamed , Saeid Naima , Mounach Samir , Berri Hicham El , Jawaldah Ayoub Al , Rahhaoui Fadoua , Mouzouni Fatima-Zahra , Rami Anass , Benjeddou Kaoutar , Lahmam Houria , Benkirane Hasnae , Elmzibri Mohammed , Kari Khalid El , Bagri Abdallah , Aguenaou Hassan , Belakhel Latifa 
Published: December 2021

Abstract
Background: Excessive sodium (Na) intake and low potassium (K) intake are associated with adverse cardiovascular health outcomes. Morocco lacks data on actual Na and K intake in adults. The aim of this study was to estimate the mean intake of Na and K in a Moroccan population of adults using the 24-h urinary excretion and to examine their association with blood pressure (BP). Methods: A total of 371 adults, who participated in the urinary validation sub-study of the STEP-wise Survey-Morocco-2017-2018, have complete data on demographic, anthropometric and blood pressure and have provided a valid 24-h urine collection according to the standard protocol of the World Health Organization (WHO). Results: The mean 24-h urinary sodium excretion was 2794 mg (SD, 1394) and the median was 2550 mg (IQR, 1780-3726). The mean 24-h urinary potassium excretion was 1898 mg (SD, 1044) and the median was 1640 mg (IQR, 1170-2410). Sodium excretion was between 3000 and 5000 mg/day in 31% of participants, < 3000 mg/day in 64%, and > 5000 mg/day in only 5%. No significant association of urinary sodium or potassium with blood pressure was found. Conclusion: Sodium intake in the studied population of Moroccan adults was higher than WHO recommendation and was comparable to levels reported in countries from Eastern Mediterranean Region. The vast majority of participants had a sodium intake < 5000 mg/day, with only 5% were above this level. Potassium intake was in the range of 1000 to 3000 mg/day. Within these ranges, there was no association between sodium or potassium intake and blood pressure. This information is crucial to help implement the national strategy to reduce sodium intake as a cost-effective intervention to prevent chronic disease in Morocco.