bluuebunny/crossref_metadata_2025_split huggingface dataset:
Prediction of Blood Pressure Change During Surgical Incision Under Opioid Analgesia Using a New Opioid Sensitivity Index: A Cross-Sectional Study
Authors: Kamiya Satoshi , Nakamura Ryuji , Saeki Noboru , Kondo Takashi , Miyoshi Hirotsugu , Narasaki Soushi , Morio Atsushi , Kawamoto Masashi , Hirano Harutoyo , Tsuji Toshio , Tsutsumi Yasuo 
Published: January 2021

Abstract
Opioid inhibition of nociceptive stimuli varies in individuals and is difficult to titrate. We have reported the vascular stiffness value (K) as a standard monitor to quantify sympathetic response with high accuracy. On the contrary, among individuals, a considerable variation in the rate of change in K for constant pain has been observed. In this study, we proposed a new index, minimum evoked current of K (MECK) and evaluated its accuracy in predicting sympathetic response to nociceptive stimuli under constant opioid administration. Thirty patients undergoing open surgery under general anesthesia were included. After anesthetic induction, remifentanil was administered at a constant concentration of 2 ng/ml at the effect site followed by tetanus stimulation. MECK was defined as the minimal current needed to produce a change in K. MECK significantly (P < 0.001) correlated with the rate of change of systolic blood pressure during skin incision (ROCBP). Bland-Altman plot analysis using the predicted ROCBP calculated from MECK and the measured ROCBP showed that the prediction equation for ROCBP was highly accurate. This study showed the potential of MECK to predict blood pressure change during surgical incision under opioid analgesia.
Suppression of Autophagy Promotes Fibroblast Activation in P53-deficient Colorectal Cancer Cells
Authors: Inoue Takanori , Hayashi Yoshito , Tsujii Yoshiki , Yoshii Shunsuke , Sakatani Akihiko , Kimura Keiichi , Uema Ryotaro , Kato Minoru , Saiki Hirotsugu , Shinzaki Shinichiro , Iijima Hideki , Takehara Tetsuo 
Published: January 2021

Abstract
Deficiency of p53 in cancer cells activates the transformation of normal tissue fibroblasts into carcinoma-associated fibroblasts; this promotes tumor progression through a variety of mechanisms in the tumor microenvironment. The role of autophagy in carcinoma-associated fibroblasts in tumor progression has not been elucidated. We aimed to clarify the significance of autophagy in fibroblasts, focusing on the TP53 status in co-cultured human colorectal cancer cell lines (TP53-wild-type colon cancer, HCT116; TP53-mutant colon cancer, HT29; fibroblast, CCD-18Co) in vitro. Autophagy in fibroblasts was significantly suppressed in association with ACTA2, CXCL12, TGFβ1, VEGFA, FGF2, and PDGFA mRNA levels, when co-cultured with p53-deficient HCT116sh p53 cells. Exosomes isolated from the culture media of HCT116sh p53 cells significantly suppressed autophagy in fibroblasts via inhibition of ATG2B. Exosomes derived from TP53-mutant HT29 cells also suppressed autophagy in fibroblasts. miR-4534, extracted from the exosomes of HCT116sh p53 cells, suppressed ATG2B in fibroblasts. In conclusion, a loss of p53 function in colon cancer cells promotes the activation of surrounding fibroblasts through the suppression of autophagy. Exosomal miRNAs derived from cancer cells may play a pivotal role in the suppression of autophagy.
A comprehensive physical interaction map between the Turnip mosaic potyvirus and Arabidopsis thaliana proteomes
Authors: Martínez Fernando , Toft Christina , Hillung Julia , Giménez-Santamarina Silvia , Yenush Lynne , Rodrigo Guillermo , Elena Santiago 
Published: January 2021

Abstract
Viruses are obligate intracellular parasites that have co-evolved with their hosts to establish an intricate network of protein-protein interactions. Yet, the systems-level mode of action of plant viruses remains poorly understood. Here, we followed a high-throughput yeast two-hybrid screening to identify 378 novel virus-host protein-protein interactions between Turnip mosaic virus (TuMV), a representative plant RNA virus, and Arabidopsis thaliana, one of its natural hosts. We found the RNA-dependent RNA polymerase NIb as the virus protein with the largest number of contacts. We verified a subset of 25 selected interactions in planta by bimolecular fluorescence complementation assays. We then constructed a comprehensive network comprising 399 TuMV-A. thaliana interactions to perform, together with intravirus and intrahost connections, detailed computational analyses. In particular, we found that the host proteins targeted by the virus participate in a higher number of infection-related functions, are more connected and have an increased capacity to spread information throughout the cell proteome, display higher expression levels, and have been subject to stronger purifying selection than expected by chance. Overall, our results provide a comprehensive mechanistic description of a plant virus-host interplay, with potential impact on disease etiology, and reveal that plant and animal viruses share fundamental features in their mode of action.
Investigation of the Invasion Mechanism Mediated by the Outer Membrane Protein PagN of Salmonella Typhimurium
Authors: Barilleau Emilie , Védrine Mégane , Koczerka Michael , Burlaud-Gaillard Julien , Kempf Florent , Grépinet Olivier , Virlogeux-Payant Isabelle , Velge Philippe , Wiedemann Agnès 
Published: January 2021

Abstract
Background. Salmonella can invade host cells via a type three secretion system called T3SS-1 and its outer membrane proteins, PagN and Rck. However, the mechanism of PagN-dependent invasion pathway used by Salmonella enterica, subspecies enterica serovar Typhimurium remains unclear.Results. Here, we report that PagN is well conserved and widely distributed among the different species and subspecies of Salmonella. We showed that PagN of S. Typhimurium was sufficient and necessary to enable non-invasive E. coli over-expressing PagN and PagN-coated beads to bind to and invade different non-phagocytic cells. According to the literature, PagN is likely to interact with heparan sulfate proteoglycan (HSPG) as PagN-mediated invasion could be inhibited by heparin treatment in a dose-dependent manner. This report shows that this interaction is not sufficient to allow the internalization mechanism. Investigation of the role of β1 integrin as co-receptor showed that mouse embryo fibroblasts genetically deficient in β1 integrin were less permissive to PagN-mediated internalization. Moreover, PagN-mediated internalization was fully inhibited in glycosylation-deficient pgsA-745 cells treated with anti-β1 integrin antibody, supporting the hypothesis that β1 integrin and HSPG cooperate to induce the PagN-mediated internalization mechanism. In addition, use of specific inhibitors and expression of dominant-negative derivatives demonstrated that tyrosine phosphorylation and class I phosphatidylinositol 3-kinase were crucial to trigger PagN-dependent internalization, as for the Rck internalization mechanism. Finally, scanning electron microscopy with infected cells showed microvillus-like extensions characteristic of Zipper-like structure, engulfing PagN-coated beads and E. coli expressing PagN, as observed during Rck-mediated internalization.Conclusions. Our results supply new comprehensions into T3SS-1-independent invasion mechanisms of S. Typhimurium and highly indicate that PagN induces a phosphatidylinositol 3-kinase signaling pathway, leading to a Zipper-like entry mechanism as the Salmonella outer membrane protein Rck.
A newly developed polydopamine-coated paper-based microchip for rapid and highly selectivity detection of foodborne pathogens
Authors: Liu Jinfeng , Zhang Mimi , Shen Zhiqiang , Liu Yongxin , Song Yang , Yu Liang , Li Zhende , Nie Lingmei , Fang Yanjun , Zhao Youquan 
Published: January 2021

Abstract
Background

In 2020, Covid-19 pneumonia has had a great impact on human health in although the countries around the world, it brings serious threaten to people’s lives and resulted in serious economic losses. At the same time, a lot news about the detection of Covid-19 in food emerges endlessly, a rapid and high selectivity detection method or technology is in urgent need for its ability to help relevant departments effectively control the epidemic situation and ensuring people’s lives and property safety. In recent years, loop-mediated isothermal amplification (LAMP) has been certified as a quick and highly selective technique to detect foodborne microorganisms.
Results

In this paper, a newly developed microchip with polydopamine-coated paper based on LAMP was fabricated. This microchip consists of nine chambers for sampling and reactions, the targeted nucleic acid of foodborne pathogens was labeled by calcein fluorescence rather than SYBR. The microchip is advantageous of lower cost of materials and simple pretreated methods, and is easy to operate without the need for complex controlled fluid flow. The LAMP procedure and fluorescence detection of pathogens can be carried on the chip without opening the lid, preventing aerosol contamination and reducing the probability of false positives. In experiments, the LAMP reaction conditions including the optimal reaction temperature and reaction time are thoroughly discussed and have been executed for various foodborne bacteria samples, including Escherichia coli O157:H7 (E. coli O157:H7), Salmonella spp., Staphylococcus aureus (S. aureus), and Vibrio parahaemolyticus (V. parahaemolyticus). Testing of E. coli O157:H7 proved to be highly selective and sensitive (as low as 0.0134 ng µL− 1). Additionally, experimental test of real milk sample was figured, the complete detection duration time was within 68 min, the limit of detection(LOD) for Salmonella spp. was determined to be lower than 12 CFU mL− 1.
Conclusion

In summary, a newly developed LAMP microchip with polydopamine-coated and calcein fluorescence labeling paper-based provides a lower cost, easy to use, highly selective, and multiplexable pathogen detection capability with great promise as a rapid, highly efficient, and economical solution for future foodborne pathogen testing.
Novel Robust Time Series Analysis for Long-term and Short-term Prediction
Authors: Okamura Hiroshi , Osada Yutaka , Nishijima Shota , Eguchi Shinto 
Published: January 2021

Abstract
Nonlinear phenomena are universal in ecology. However, their inference and prediction are generally difficult because of autocorrelation and outliers. A traditional least squares method for parameter estimation is capable of improving short-term prediction by estimating autocorrelation, whereas it has weakness to outliers and consequently worse long-term prediction. In contrast, a traditional robust regression approach, such as the least absolute deviations method, alleviates the influence of outliers and has potentially better long-term prediction, whereas it makes accurately estimating autocorrelation difficult and possibly leads to worse short-term prediction. We propose a new robust regression approach that estimates autocorrelation accurately and reduces the influence of outliers. We then compare the new method with traditional least squares and least absolute deviations methods using simulated data and real ecological data. Simulations and analysis of real data demonstrate that the new method generally has better long-term and short-term prediction ability for nonlinear estimation problems using spawner--recruitment data. The new method provides nearly unbiased autocorrelation even for highly contaminated simulated data with extreme outliers, whereas the other methods fail to estimate autocorrelation accurately.
Application of Self-Pulling and Latter Transection in Totally Laparoscopic Total Gastrectomy
Authors: Zhao Yang , Bai Zhi-Xia , Wang Tao , Song Dong , Li Tao 
Published: January 2021

Abstract
Background: To investigate the safety and efficacy of self-pulling and latter transection in totally laparoscopic total gastrectomy (SPLT-TLTG). Methods: Eighty patients with gastric cancer who received either SPLT-TLTG or laparoscopic-assisted total gastrectomy (LATG) were enrolled for this study from January 2016 to June 2018. Clinical data including clinicopathologic parameters, postoperative conditions and long-term prognosis were collected and compared between patients received different types of surgeries. Results: Compared to LATG, patients who received SPLT-TLTG surgery were associated with shorter operation time, less intraoperative blood loss and smaller incision lesion. In addition, patients who received SPLT-TLTG surgeries spent significantly less time in bed-rest post-surgically, to first bowel movement, hospital stay and before first oral food-intake (P < 0.05), as long as lower postoperative pain scores. However, no significant difference was observed between SPLT-TLTG and LATG surgeries in terms of how many lymph nodes (LNs) were retrieved during operation, as well as the overall incidence of postoperative complications (P > 0.05). Conclusions: This pilot study provided primary evidence for the application of self-pulling and latter transection in totally laparoscopic total gastrectomy in the treatment of gastric cancer.
Policy Discourse: Shifting the burden of healthcare from state to market in the Kingdom of Saudi Arabia
Authors: Rahman Redwanur , Salam Mohammed Asif 
Published: January 2021

Abstract
Saudi Arabia has modified from a predominantly free, public and comprehensive system under a welfare model to more of a mixed-economy model of healthcare. The welfare state slowly moved to a liberal model, emphasising market forces to dominate, and the private sector was trusted to provide better provision of healthcare. The country has confronting enormous problems in the health sector due to population growth, lifestyle changes, shift of disease pattern, elevated expectations, escalated healthcare costs, limited infrastructure and resources, and poor management practice in the provision of healthcare. Moreover, the government has been emphasizing on the need to bring in private sector investment to improve quality and efficiency, development of manpower, and standardization of services. As the current pattern of healthcare is unsustainable, the country is planning to restructure the present healthcare system towards institutionalizing it to meet the future challenges. The governments must make an appropriate amount of effort to build its healthcare systems by transforming and modifying the challenges faced by the society and its political-economic systems. The government should encourage equity, and fairness in the provision of healthcare.
S100A14 Induces Apoptosis and Negatively Regulates Proliferation of Nasopharyngeal Carcinoma Cells
Authors: Xiao Yu , Han Jibo , Li Fen , Zheng Anyuan , Chen Qibing , Chen Fuhai , Cheng Xiang , Tao Zezhang 
Published: January 2021

Abstract
Background: S100A14 is involved in multiple pathological processes; however, its role in nasopharyngeal carcinoma is poorly understood. Methods: S100A14 was deleted or upregulated in 6-10B cells. Results: S100A14-knockdown 6-10B cells showed significantly higher optical density values in the CCK-8 assay, smaller scratch width in the scratch experiment, and significantly more invading cells in the transwell assay compared with controls. Compared with the control group, the G2/M and S phase proportions of the S100A14-overexpression group were significantly higher, early apoptosis was observed via JC-1 fluorescence, and flow cytometry showed a significantly higher proportion of apoptotic cells. Protein expression of Bcl-2 and Bcl-xl decreased significantly, whereas that of Bax, Bad, cleaved-PARP, and cleaved-caspase-3/9 increased. Conclusions: Knockdown of S100A14 promoted proliferation, migration, and invasion of 6-10B cells, whereas its upregulation promoted caspase-dependent apoptosis and induced S and G2/M phase arrest, indicating a role of S100A14 as a tumor suppressor gene in nasopharyngeal carcinoma.
A Transcription Factor Signature Predicts Prognosis of Patients with Adrenocortical Carcinoma
Authors: Zhao Jianyu , Liu Bo , Li Xiaoping 
Published: January 2021

Abstract
Background: Adrenocortical carcinoma (ACC) is a rare endocrine cancer that manifests as abdominal masses and excessive steroid hormone levels. Transcription factors (TFs) deregulation is found to be involved in adrenocortical tumorigenesis and cancer progression. This study aimed to construct a TF-based prognostic signature for prediction of survival of ACC patients.Results: We identified a 13-TF prognostic signature comprised of CREB3L3, NR0B1, CENPA, FOXM1, E2F2, MYBL2, HOXC11, ZIC2, ZNF282, DNMT1, TCF3, ELK4, and KLF6 using the univariate Cox analysis and LASSO Cox regression. The risk score based on the TF-signature could classify patients into low- and high-risk group. Kaplan-Meier analyses showed that patients in the high-risk group had significantly shorter overall survival compared to the low-risk patients. ROC curves showed that the prognostic signature predicted the overall survival of ACC patients with good sensitivity and specificity. Furthermore, the TF-risk score was an independent prognostic factor.Conclusion: Taken together, we identified a 13-TF prognostic marker to predict overall survival in ACC patients.
Association Between Circulating Adiponectin and Heart Rate Recovery in Women With Polycystic Ovarian Syndrome
Authors: Zheng Huan , Li Zhiling , Feng Wei , Liu Bin 
Published: January 2021

Abstract
Background: Imbalance of autonomic nervous system is confirmed as a key contributor for cardiometabolic complications in polycystic ovarian syndrome (PCOS) women. Heart rate recovery (HRR) is an easy test for autonomic nervous tone evaluation and a powerful index for predicting cardiovascular events and mortality. Adiponectin (APN) is reported to be correlated closely with autonomic nervous function in different populations.Methods: This study aimed to investigate the relationship between serum total APN and HRR in PCOS women. A total of 89 PCOS women were enrolled and divided into two groups. Women with HRR values slower than 12 beats were defined as Blunted HRR Group. APN levels were compared between Blunted HRR Group and Normal HRR Group. Multivariate logistic regression analysis and multiple linear regression analysis were performed to determine which clinical variables were independently associated with decreased HRR and the effect of clinical variables on APNlevels, respectively. Results: 23 women were categorized in Blunted HRR Group, in which the APN level was significantly lower than Normal HRR Group (10.2±3.9 ug/ml vs. 13.1±4.1 ug/ml, P=0.015). Age [odds ratio (OR) = 1.15; 95% confidence interval (CI) = 1.04-1.21; P = 0.032], BMI (OR = 0.93; 95% CI = 0.72-1.08; P = 0.006), hypertension (OR = 1.08; 95%CI = 1.02-1.26; P = 0.025) and APN (OR= 0.61; 95%CI = 0.43-0.88; P = 0.018) were independent factors of attenuated HRR in PCOS women. Meanwhile, multiple linear regression analysis showed only age (β=-0.26; 95%CI =-0.47- -0.03, P= 0.041) and hyperlipemia (β=-0.13; 95%CI =-0.27-0.08, P= 0.024) were closely associated with APN levels in PCOS women.Conclusions: Our findings suggested that decreased APN concentration was closely associated with HRR blunt in PCOS women. Further studies are needed to explore the underlying interactions between APN and autonomic nervous function.
CASK Silence Enhances Chemosensitivity of Hepatocellular Carcinoma Through activating Apoptosis, Inducing JNK/c-Jun-Mediated Autophagic Cell Death and Decreasing ABCG2
Authors: Ding BiSha , Bao Chang , Jin Luqi , Xu Liang , Dai Zhijun , Fan Weimin , Lou Weiyang 
Published: January 2021

Abstract
Background: Advanced hepatocellular carcinoma (HCC) patients usually fail to be treated because of drug resistance, including sorafenib. Methods: The expression and prognostic role of calcium/calmodulin-dependent serine protein kinase (CASK) in HCC were assessed by combination of bioinformatic analysis and experimental validation. The effects of CASK in regulating proliferation, apoptosis and drug resistance of HCC cells in vitro and in vivo were investigated using gain- or loss-of-function strategies by performing lots of specific methods including Cell Counting kit-8 (CCK8), colony formation assay, flow cytometry, transmission electron microscopy, immunofluorescent confocal laser microscopy and tumor xenograft experiments, immunohistochemistry staining. Moreover, the underlying molecular mechanisms responsible for CASK’s functions in HCC were also explored. Results: Currently, we discovered that CASK was positively associated with sorafenib resistance of HCC in vitro and in vivo, and was significantly related with poor prognosis in HCC. Moreover, inhibition of CASK can increase the effect of sorafenib partially by promoting apoptosis and autophagy, while CASK overexpression presented the opposite results. Besides, all the pan-caspase inhibitor Z-VAD-FMK, autophagy inhibitor 3-Methyladenine (3-MA) and small interfering RNA (siRNA) of LC3B reversed CASK knockout-induced effects with sorafenib treatment, suggesting that both apoptosis and autophagy were involved in CASK-mediated above functions and autophagy played a pro-death role in this research. Intriguingly, similar results were observed in vivo. In molecular level, CASK knockout activated the c-Jun N-terminal kinase (JNK) pathway, and treatment with JNK inhibitor SP600125 or transiently transfected with si-JNK significantly attenuated CASK knockout-mediated autophagic cell death. Besides, knockout of CASK dramatically inhibited the expression of ATP binding cassette subfamily G member 2 (ABCG2) and reversed of multidrug-resistance (MDR) of HCC. Conclusions: Collectively, all these results together indicated that CASK might be a promising biomarker for HCC patients and a potential therapeutic target for relieving drug resistance of HCC.