bluuebunny/crossref_metadata_2025_split huggingface dataset:
Vitamin D inhibits TNF-α induced apoptosis of human nucleus pulposus cells through regulation of NF-kB signaling pathway
Authors: Zhang Cun , Tong Tong , Miao De-chao , Wang Lin-feng 
Published: June 2021

Abstract
Background
To observe the effects of vitamin D on the apoptotic human nucleus pulposus cells under tumor necrosis factor-α (TNF-α) treatment.

Methods
The gene expression data was downloaded from the NCBI Gene Expression Omnibus (GEO) database (https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE34095). Differentially expressed genes between degenerative disc and non-degenerative disc were performed by R software. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genome (KEGG) pathway enrichment analyses were performed using The Database for Annotation, Visualization and Integrated Discovery (DAVID). Then, the human nucleus pulposus tissue was harvested from 12 patients according to the modified Pfirrmann classification and human nucleus pulposus cells were obtained from digestion of herniated nucleus pulposus tissue. The collected nucleus pulposus cells were treated with different concentration of TNF-α, and cellular apoptosis was measured by flow cytometry. Then, human nucleus pulposus cells were divided into following groups: normal culture medium, TNF-α treated, TNF-α, and vitamin D-treated groups. Cellular apoptosis rate was quantified by flow cytometry. Protein expression of p-p65, p65, and IkBa was detected with western blot analysis.

Results
A total of 536 differentially expressed genes were identified through bioinformatic analysis. KEGG pathway revealed that NF-kB signaling pathway was involved in the process of disc degeneration. In the NP cell cultures, vitamin D significantly increased cell proliferation potency. Furthermore, vitamin D inhibited TNF-α induced apoptosis of human nucleus pulposus cells. Vitamin D reduced the phospho-NF-κB/p65 expression in the TNF-α-treated NP cells.

Conclusion
Vitamin D can attenuate TNF-α-induced NP cells apoptosis through interfering with the NF-κB pathway.
The voltage-gated proton channel Hv1 promotes microglia-astrocyte communication and neuropathic pain after peripheral nerve injury
Authors: Peng Jiyun , Yi Min-Hee , Jeong Heejin , McEwan Przemyslaw Peter , Zheng Jiaying , Wu Gongxiong , Ganatra Shashank , Ren Yi , Richardson Jason R. , Oh Seog Bae , Wu Long-Jun 
Published: June 2021

AbstractActivation of spinal cord microglia contributes to the development of peripheral nerve injury-induced neuropathic pain. However, the molecular mechanisms underlying microglial function in neuropathic pain are not fully understood. We identified that the voltage-gated proton channel Hv1, which is functionally expressed in spinal microglia, was significantly increased after spinal nerve transection (SNT). Hv1 mediated voltage-gated proton currents in spinal microglia and mice lacking Hv1 (Hv1 KO) display attenuated pain hypersensitivities after SNT compared with wildtype (WT) mice. In addition, microglial production of reactive oxygen species (ROS) and subsequent astrocyte activation in the spinal cord was reduced in Hv1 KO mice after SNT. Cytokine screening and immunostaining further revealed that IFN-γ expression was compromised in spinal astrocytes in Hv1 KO mice. These results demonstrate that Hv1 proton channel contributes to microglial ROS production, astrocyte activation, IFN-γ upregulation, and subsequent pain hypersensitivities after SNT. This study suggests Hv1-dependent microglia-astrocyte communication in pain hypersensitivities and identifies Hv1 as a novel therapeutic target for alleviating neuropathic pain.
Role of bulk nanobubbles in removing organic pollutants in wastewater treatment
Authors: Wu Jiajia , Zhang Kejia , Cen Cheng , Wu Xiaogang , Mao Ruyin , Zheng Yingying 
Published: June 2021

AbstractThe occurrence of a variety of organic pollutants has complicated wastewater treatment; thus, the search for sustainable and effective treatment technology has drawn significant attention. In recent years, bulk nanobubbles, which have extraordinary properties differing from those of microbubbles, including high stability and long residence times in water, large specific surface areas, high gas transfer efficiency and interface potential, and the capability to generate free radicals, have shown attractive technological advantages and promising application prospects for wastewater treatment. In this review, the basic characteristics of bulk nanobubbles are summarized in detail, and recent findings related to their implementation pathways and mechanisms in organic wastewater treatment are systematically discussed, which includes improving the air flotation process, increasing water aeration to promote aerobic biological technologies including biological activated carbon, activated sludge, and membrane bioreactors, and generating active free radicals that oxidise organic compounds. Finally, the current technological difficulties of bulk nanobubbles are analysed, and future focus areas for research on bulk nanobubble technology are also proposed.
Cardiac effects and clinical applications of MG53
Authors: Zhong Weina , Benissan-Messan Dathe Z. , Ma Jianjie , Cai Chuanxi , Lee Peter H. U. 
Published: June 2021

AbstractHeart disease remains the leading cause of mortality globally, so further investigation is required to identify its underlying mechanisms and potential targets for treatment and prevention. Mitsugumin 53 (MG53), also known as TRIM72, is a TRIM family protein that was found to be involved in cell membrane repair and primarily found in striated muscle. Its role in skeletal muscle regeneration and myogenesis has been well documented. However, accumulating evidence suggests that MG53 has a potentially protective role in heart tissue, including in ischemia/reperfusion injury of the heart, cardiomyocyte membrane injury repair, and atrial fibrosis. This review summarizes the regulatory role of MG53 in cardiac tissues, current debates regarding MG53 in diabetes and diabetic cardiomyopathy, as well as highlights potential clinical applications of MG53 in treating cardiac pathologies.
Reconstruction of ocean environment time series since the late nineteenth century using sclerosponge geochemistry in the northwestern subtropical Pacific
Authors: Asami Ryuji , Matsumori Taketo , Shinjo Ryuichi , Uemura Ryu , Miyaoka Yuki , Mizuyama Masaru , Ise Yuji , Sakamaki Takashi 
Published: June 2021

AbstractThe geochemistry of calcifying marine organisms is an excellent proxy for reconstructing paleoceanographic history, but studies of hypercalcified demosponges (sclerosponges) are considerably fewer than those of corals, foraminifers, and bivalves. For this study, we first generated near-annual resolved stable carbon and oxygen isotope (δ13C and δ18O) and element/Ca ratios (Sr/Ca, Ba/Ca, Pb/Ca, U/Ca) time series for 1880–2015 from sclerosponge samples (Acanthochaetetes wellsi) collected at Miyako Island and Okinawa Island in the Ryukyu Islands of southwestern Japan. The δ13C records exhibited a typical variation of anthropogenically derived Suess effects, demonstrating that the rates of decrease of –0.0043‰/year before 1960 and – 0.024‰/year after 1960 in the northwestern subtropical Pacific were respectively similar to and about 1.4 times higher than those of the Caribbean Sea in the tropical Atlantic. Spectral analysis of the δ18O time series revealed significant periodicity of approximately 2, 3, 6.5, 7–10, and 20–30 year/cycle, indicating that sea surface conditions in the southern Ryukyu Islands had been dominated by interannual and decadal variations in temperature and seawater δ18O since the late nineteenth century. The Sr/Ca and U/Ca ratios for the species A. wellsi (high-Mg calcite) might not be a robust proxy for seawater temperatures, unlike Astrosclera willeyana and Ceratoporella nicholsoni sclerosponges (aragonite). An evident increasing Pb/Ca trend after 1950 found in the samples is probably attributable to Pb emissions from industrial activities and atmospheric aerosols in eastern Asian countries. The Ba/Ca variations differ greatly among sampling sites, which might be attributable to the respective local environments. This evidence demonstrates that more high-resolution age determinations and geochemical profilings enable delineation of secular variations in ocean environments on annual and interannual timescales. Results of our study suggest that if sclerosponges living in deeper ocean environments are collected, spatial and vertical oceanographic variations for the last several centuries will be reconstructed along with coral proxy records.
Colorectal cancer in Crohn’s disease: a series of 6 cases
Authors: Ishimaru Kazuhide , Tominaga Tetsuro , Nonaka Takashi , Fukuda Akiko , Moriyama Masaaki , Oyama Shosaburo , Ishii Mitsutoshi , Sawai Terumitu , Nagayasu Takeshi 
Published: June 2021

Abstract
Background
Colorectal cancer (CRC) is the most malignant complication in patients with Crohn’s disease (CD). We report 6 cases of CD-related CRC treated surgically at our hospital.

Case presentation
From 2010 to 2016, six CD patients were diagnosed with CRC. All patients were diagnosed with CD at < 25 years old, and the interval from onset of CD to diagnosis of CRC was > 10 years (range, 15–42 years) in all patients. The histological type of cancer was mucinous carcinoma in two cases, well-differentiated tubular adenocarcinoma in two cases, and moderately differentiated tubular adenocarcinoma in two cases. CRC was detected by screening colonoscopy in three cases (50%), and from clinical symptoms in the remaining three cases (50%). Two cases underwent colonoscopy within 2 months after symptom onset, detecting CRC in the relatively early stage. However, one case was diagnosed with advanced-stage CRC by endoscopy 1 year after symptom onset, and experienced poor prognosis.

Conclusions
Regular surveillance colonoscopy is needed to detect early-stage CRC in CD patients. Clear surveillance methods need to be established based on evidence.
Disparate access to nutritional food; place, race and equity in the United States
Authors: Sansom Garett , Hannibal Bryce 
Published: June 2021

Abstract
Background
Prior research has demonstrated minority communities have fewer options to access healthy foods when compared to their majority counterparts. While much focus has been placed upon community-level resources, little research has been placed on the efforts that minority groups need to undergo to reach well-stocked stores to purchase healthy food options.

Methods
As part of the Water, Energy, Food Nexus Research Group at Texas A&M University, a nationally representative survey (n = 1612) was conducted to acquire self-reported distance, time, and motives that certain populations must travel to purchase food for themselves and their families.

Results
Findings suggest that minority populations consider saving money, driving less, having a better selection of foods, and have the ability to buy organic foods as an important factor when choosing where to buy foods. Further, minority populations across the nation need to drive a significantly greater (p < 0.05) amount of time to reach their destinations than white populations.

Conclusion
This underscores the importance, and scope of the issues, of promoting and implementing more equitably distributed opportunities to purchase healthy food options throughout the United States.
Combined soft measurement on key indicator parameters of new competitive advantages for China's export
Authors: Wang Taosheng , Zuo Hongyan , Wu C. H. , Hu B. 
Published: June 2021

AbstractThe estimation of the difference between the new competitive advantages of China's export and the world’s trading powers have been the key measurement problems in China-related studies. In this work, a comprehensive evaluation index system for new export competitive advantages is developed, a soft-sensing model for China’s new export competitive advantages based on the fuzzy entropy weight analytic hierarchy process is established, and the soft-sensing values of key indexes are derived. The obtained evaluation values of the main measurement index are used as the input variable of the fuzzy least squares support vector machine, and a soft-sensing model of the key index parameters of the new export competitive advantages of China based on the combined soft-sensing model of the fuzzy least squares support vector machine is established. The soft-sensing results of the new export competitive advantage index of China show that the soft measurement model developed herein is of high precision compared with other models, and the technical and brand competitiveness indicators of export products have more significant contributions to the new competitive advantages of China's export, while the service competitiveness indicator of export products has the least contribution to new competitive advantages of China's export.
TAVR and cancer: machine learning-augmented propensity score mortality and cost analysis in over 30 million patients
Authors: Monlezun Dominique J. , Hostetter Logan , Balan Prakash , Palaskas Nicolas , Lopez-Mattei Juan , Cilingiroglu Mehmet , Iakobishvili Zaza , Ewer Michael , Marmagkiolis Konstantinos , Iliescu Cezar 
Published: June 2021

Abstract
Introduction
Cardiovascular disease (CVD) and cancer are the top mortality causes globally, yet little is known about how the diagnosis of cancer affects treatment options in patients with hemodynamically compromising aortic stenosis (AS). Patients with cancer often are excluded from aortic valve replacement (AVR) trials including trials with transcatheter AVR (TAVR) and surgical AVR (SAVR). This study looks at how cancer may influence treatment options and assesses the outcome of patients with cancer who undergo SAVR or TAVR intervention. Additionally, we sought to quantitate and compare both clinical and cost outcomes for patients with and without cancer.

Methods
This population-based case-control study uses the most recent year available National Inpatient Sample (NIS (2016) from the United States Department of Health and Human Services’ Agency for Healthcare Research and Quality (AHRQ). Machine learning augmented propensity score adjusted multivariable regression was conducted based on the likelihood of undergoing TAVR versus medical management (MM) and TAVR versus SAVR with model optimization supported by backward propagation neural network machine learning.

Results
Of the 30,195,722 total hospital admissions, 39,254 (0.13%) TAVRs were performed, with significantly fewer performed in patients with versus without cancer even in those of comparable age and mortality risk (23.82% versus 76.18%, p < 0.001) despite having similar hospital and procedural mortality. Multivariable regression in patients with cancer demonstrated that mortality was similar for TAVR, MM, and SAVR, though LOS and cost was significantly lower for TAVR versus MM and comparable for TAVR versus SAVR. Patients with prostate cancer constituted the largest primary cancer among TAVR patients including those with metastatic disease. There were no significant race or geographic disparities for TAVR mortality.

Discussion
Comparison of aortic valve intervention in patients with and without cancer suggests that interventions are underutilized in the cancer population. This study suggests that patients with cancer including those with metastasis have similar inpatient outcomes to patients without cancer. Further, patients who have symptomatic AS and those with higher risk aortic valve disease should be offered the benefit of intervention. Modern techniques have reduced intervention-related adverse events, provided improved quality of life, and appear to be cost effective; these advantages should not necessarily be denied to patients with co-existing cancer.
Development and implementation of an ultralow-dose CT protocol for the assessment of cerebrospinal shunts in adult hydrocephalus
Authors: Ryan David J. , Kavanagh Richard G. , Joyce Stella , O’Callaghan Maher Mika , Moore Niamh , McMahon Aisling , Hussey Deirdre , O’Sullivan Michael G. J. , Wyse Gerald , Fanning Noel , O’Connor Owen J. , Maher Michael M. 
Published: June 2021

Abstract
Background
Cerebrospinal fluid shunts in the treatment of hydrocephalus, although associated with clinical benefit, have a high failure rate with repeat computed tomography (CT) imaging resulting in a substantial cumulative radiation dose. Therefore, we sought to develop a whole-body ultralow-dose (ULD) CT protocol for the investigation of shunt malfunction and compare it with the reference standard, plain radiographic shunt series (PRSS).

Methods
Following ethical approval, using an anthropomorphic phantom and a human cadaveric ventriculoperitoneal shunt model, a whole-body ULD-CT protocol incorporating two iterative reconstruction (IR) algorithms, pure IR and hybrid IR, including 60% filtered back projection and 40% IR was evaluated in 18 adult patients post new shunt implantation or where shunt malfunction was suspected. Effective dose (ED) and image quality were analysed.

Results
ULD-CT permitted a 36% radiation dose reduction (median ED 0.16 mSv, range 0.07–0.17, versus 0.25 mSv (0.06–1.69 mSv) for PRSS (p = 0.002). Shunt visualisation in the thoracoabdominal cavities was improved with ULD-CT with pure IR (p = 0.004 and p = 0.031, respectively) and, in contrast to PRSS, permitted visualisation of the entire shunt course (p < 0.001), the distal shunt entry point and location of the shunt tip in all cases. For shunt complications, ULD-CT had a perfect specificity. False positives (3/22, 13.6%) were observed with PRSS.

Conclusions
At a significantly reduced radiation dose, whole body ULD-CT with pure IR demonstrated diagnostic superiority over PRSS in the evaluation of cerebrospinal fluid shunt malfunction.
Role of preoperative ultrasound mapping in the surgical management of deep infiltrating endometriosis: a prospective observational study
Authors: El-Maadawy Samar M. , Alaaeldin Nesreen , Nagy Charles B. 
Published: June 2021

Abstract
Background
Endometriosis is a challenging gynecological condition that has a profound influence on the quality of life of affected women. Transvaginal ultrasound is considered the first-line imaging method in preoperative assessment of the extent and severity of endometriosis. Accurate preoperative mapping can aid the surgeon in patient counselling, selection of the most appropriate surgical method that minimizes the operative and post-operative complications. The aim of our study is to evaluate the accuracy of transvaginal sonography (TVS) in precisely assessing the size, location and extent of deep infiltrating endometriosis (DIE) using a new modified endometriosis mapping proforma with histopathological confirmation. Our prospective observational study included 101 women with clinically suspected DIE who underwent TVS followed by laparoscopy from October 2018 to December 2020 with a maximum of 4 weeks interval. Precise mapping of DIE was done during TVS and laparoscopy. Results were correlated with histopathology findings.

Results
DIE was confirmed by histopathology in 88 patients. Sensitivity and specificity for individual DIE locations were rectovaginal septum 67.9% and 98.6%; vagina 52.2% and 98.7%; uterosacral ligaments 82.5% and 96.2%; torus 96.4% and 97.3%; parametrium 68.8% and 96.9%; rectum 100% and 98.8%; bladder 100% and 100%, ureters 63.4% and 99.0%; scar endometriosis 100% and 100%; pouch of Douglas obliteration 97.7% and 100%. No statistically significant difference was detected between ultrasound and histopathology size. Ultrasound tended to underestimate the lesion size; the underestimation was more pronounced for lesions > 3 cm. “Butterfly” and “tram-track” signs are two new sonographic signs related to posterior compartment DIE. No post-operative complications were recorded. There were no cases of DIE recurrence. Eleven out of 22 cases of infertility achieved pregnancy during 18 months follow-up.

Conclusion
TVS provides a thorough and accurate evaluation of the extent of endometriosis. An experienced radiologist can use E-PEP to provide an accurate demonstration of the location and extent of DIE which helps the surgeon select the most appropriate surgical approach ensuring radical treatment of the disease and minimizing short- and long-term complications.
Evaluation of HCV-related liver fibrosis post-successful DAA therapy
Authors: Ehsan Nermine , Sweed Dina , Elsabaawy Maha 
Published: June 2021

Abstract
Background
The rapidly developing era of direct-acting antiviral regimens (DAAs) for more than one hepatitis C virus (HCV) genotype had certainly alleviated HCV burden all over the world. Liver fibrosis is the major dramatic complication of HCV infection, and its progression leads to cirrhosis, liver failure, and hepatocellular carcinoma. The impact of DAAs on liver fibrosis had been debatably evaluated with undetermined resolution.

Main body
The aim of this review is to accurately revise the effects of DAA regimens on liver fibrosis which can either be regression, progression, or non-significant association. Liver fibrosis regression is a genuine fact assured by many retrospective and prospective clinical studies. Evaluation could be concluded early post-therapy reflecting the dynamic nature of the process.

Conclusions
The ideal application of DAA regimens in treating HCV has to be accomplished with efficient non-invasive markers in differentiating proper fibrosis evaluation from necroinflammation consequences. Liver biopsy is the gold standard that visualizes the dynamic of fibrosis regression.
In search of America’s old covered bridges
Authors: Knapp Ronald G. 
Published: June 2021

AbstractAmerica’s first documented wooden covered bridge was erected at Philadelphia, Pennsylvania in 1805. Hundreds were constructed within two decades and at least 10,000 by the later 1800s. As settlers moved West, broad rivers were crossed with inventive structures incorporating timber trusses ingeniously developed by carpenters. Called covered bridges because of the roof and siding needed to protect the timber trusses, they became ubiquitous features on the American landscape. Over the past two centuries, most covered bridges were lost to flood, ice, arson, lightening, decay, as well as “progress,” replaced by “modern” iron, concrete, and steel spans. Of some 700 covered bridges remaining, many are mere replicas of their original forms no longer supported by timber trusses. Genuine historic bridges remain largely from the last half of the 1800s while civic boosterism has led to claims of earlier dates with often questionable authenticity. This essay presents three wooden covered bridges constructed in the 1820s along a 10-mile stretch of the Wallkill River in New Paltz, New York. Of the three, only Perrine’s Bridge, constructed first in 1821 and covered in 1822, is still standing with intact Burr timber trusses. Perrine’s is an iconic structure with exceptional heritage value because of authentic re-building and restoration in 1834, 1846, 1917, and 1968. Using documentary records, this essay establishes an accurate intertwined chronology for the three bridges, detailing nineteenth century building practices and contentious mid-twentieth century struggles pitting preservationists wanting authentic restoration against those wanting removal.