bluuebunny/crossref_metadata_2025_split huggingface dataset:
ZSCAN18 Promoter is Hypermethylated and Its Restored Expression Inhibits Proliferation in Gastric Cancer
Authors: Ren Baoqing , Li Bin , Ma Gang , Cai Fenglin , Wang Pengliang , Dong Yinping , Zeng Yi , Liu Yong , Zhang Li , Yang Yang , Liang Han , Zhang Nannan , Nie Yongzhan , Deng Jingyu 
Published: December 2021

Abstract
Background: Zinc finger and scan domain containing 18 (ZSCAN18) belongs to the zinc finger transcription factor superfamily, which consists of hundreds of members that play critical roles in all steps of tumorigenesis. Although hypermethylation of the ZSCAN18 promoter and its consequent deficiency in a variety of malignancies have been reported recently, its functions and mechanisms in the initiation and progression of gastric cancer (GC) are not clear. This study aims to investigate the roles of ZSCAN18 in gastric carcinogenesis.Methods: Methylation of ZSCAN18 promoter in GC cell lines was analyzed via MassARRAY and treatment with 5-aza-2′-deoxycytidine. Bioinformatics analysis, quantitative real-time polymerase chain reaction (qRT-PCR), western blot, immunohistochemistry, Cell Counting Kit 8, colony formation, and an in vivo xenograft tumor model were used to examine the expression and function of ZSCAN18. Survival analysis was performed to determine the correlation between ZSCAN18 expression and the prognosis of patients with GC. Genes modulated by ZSCAN18 in NCI-N87 cells were identified through RNA next-generation sequencing and verified by qRT-PCR in AGS and NCI-N87 cells.Results: ZSCAN18 expression was markedly reduced in GC tissues compared with adjacent normal tissues as a result of hypermethylation in GC. Likewise, ZSCAN18 expression was significantly reduced in a panel of GC cell lines as a result of the densely methylated ZSCAN18 promoter. Forced expression of ZSCAN18 inhibited proliferation in AGS and NCI-N87 cells in vitro. Additionally, ZSCAN18 impaired the growth of NCI-N87 cells in immunodeficient mice. Survival analysis showed that ZSCAN18 expression was positively associated with favorable outcomes in patients with GC. RNA next-generation sequencing showed three representative genes (FGF20, CEACAM5, and TP53INP2) involved in downstream signaling pathways modulated by ZSCAN18.Conclusions: Collectively, this study unveils the anti-cancer role of ZSCAN18 in GC and provides a promising diagnostic and therapeutic target.
New Insight Into The Spatial Autoregressive Model of Industrial Wastewater Discharge In The Yellow River Basin
Authors: Xia Libo , Wang Zhiliang , Du Shuang , Tian Decun , Chen Feng 
Published: December 2021

Abstract
This article has carried out a statistical analysis of the industrial wastewater discharge (IWD) and gross regional product (GRP) of 79 cities in the Yellow River Basin from 2003 to 2019. By calculating the Moran index of IWD and GRP, the study has found that a certain spatial autoregressive in space. There is an environmental Kuznets curve (EKC) between the environmental pollution and economic development of cities in the Yellow River Basin, and a spatial autoregressive is modelled by a set of random effects that are assigned a conditional autoregressive prior distribution. In the Bayesian environment, Markov chain Monte Carlo (MCMC) is used for inferencing, and the spatial weight matrix is ​​selected to be U-shaped matrix, and the error of the model is minimized. The parameter posterior distribution results of the model showed that the GRP did not show a significant decline. The modified EKC showed that the discharge of industrial wastewater in the entire Yellow River Basin will be reduced. Generally, cities with high pollutant emissions should learn from other cities to reduce emissions, and cities with low GRP need to increase local economic development.
Physical activity and sedentary behavior; mechanistic insights and role in disease prevention
Authors: Hoed Marcel den , Wang Zhe , Emmerich Andrew , Pillon Nicolas , Moore Timothy , Hemerich Daiane , Cornelis Marilyn , Mazzaferro Eugenia , Broos Siacia , Ameur Adam , Bandinelli Stefania , Bis Joshua , Boehnke Michael , Bouchard Claude , Chasman Daniel , de Geus Eco , Deldicque Louise , Dörr Marcus , Evans Michele , Ferrucci Luigi , Fornage Myriam , Fox Caroline , Garland Theodore , Gyllensten Ulf , Hansen Torben , Hayward Caroline , Horta Bernardo , Hypponen Elina , Johnson W. Craig , Kardia Sharon , Kiemeney Lambertus , Laakso Markku , Langenberg Claudia , Lehtimäki Terho , Marchand Loic Le , Magnusson Patrik , Martin Nicholas , Melbye Mads , Metspalu Andres , Meyre David , North Kari , Ohlsson Claes , Oldehinkel Albertine , Orho-Melander Marju , Pare Guillaume , Park Taesung , Pedersen Oluf , Penninx Brenda , Pers Tune , Polasek Ozren , Prokopenko Inga , Rotimi Charles , Samani Nilesh , Sim Xueling , Smith George Davey , Snieder Harold , Sorensen Thorkild , Spector Tim , Timpson Nicholas , Dam Rob van , Velde Nathalie van der , Vollenweider Peter , Völzke Henry , Voortman Trudy , Waeber Gerard , Wareham Nick , Weir David , Wichmann Erich , Wilson James , Hevener Andrea , Krook Anna , Zierath Juleen , Thomis Martine , Loos Ruth 
Published: December 2021

Abstract
Even though physical activity and sedentary behavior are moderately heritable, little is known about the mechanisms that influence these traits. Here, we combine data for up to 674,980 individuals from 51 studies in a trans-ancestry meta-analysis of genome-wide association studies for self-reported moderate-to-vigorous intensity physical activity during leisure time (MVPA); leisure screen time (LST); sedentary commuting; and sedentary behavior at work. We identify 99 loci that associate with at least one trait. Loci associated with LST are enriched for genes whose expression in skeletal muscle is altered by resistance training. Molecular dynamics simulations suggest that the Glu to Ala substitution encoded by rs2229456 (ACTN3) – associated with more MVPA – disrupts salt bridge interactions and makes the alpha actinin 3 filaments more flexible. In isolated type IIA muscle fibers, the Ala-encoding allele is associated with lower maximal force and power during an isometric contraction, suggesting protection from exercise-induced muscle damage. Finally, Mendelian Randomization analyses show that the causal effect of LST on BMI is 2-3 times larger than the effect of body mass index (BMI) on LST, and that beneficial effects of LST and MVPA on several risk factors and diseases are mediated or confounded by BMI. Taken together, our results provide mechanistic insights into the regulation of MVPA and into the role of LST and MVPA in disease prevention. These insights may facilitate the development of tailored physical activity interventions.
CD138 Expression Promotes Accumulation and Activation of T Cells in Autoimmune MRL/lpr Mice
Authors: Xie Tianhong , Liu Xin , Li Ping 
Published: December 2021

Abstract
CD138+ T cells that accumulated in Fas-deficiency lupus mice, had been identified as autoreactive T cells in SLE which significantly promoted autoantibody secretion. In present study, we found CD138 expression in T cells played a key role in the progression of SLE in MRL/lpr mice. Our results indicated CD138+ T cells apoptosis was in Fas dependent way. However, CD138 expression of T cells in MRL/lpr mice could significantly prevent T cells apoptosis, contribute to accumulation of T cells and DN T cells and simultaneously promote T cells activation. Importantly, CD138 expression in DN T cells significantly increased FasL expression of DN T cells enhancing the cytotoxity of DN T cells. Phorbol 12-myristate 13-acetate and Ionomycin (PI) stimulation could significantly prevent CD138+ T cells accumulation by strikingly inducing their specific apoptosis. Moreover, PI stimulation significantly activated CD138+ T cells with increased CD69 expression in them. Importantly, our results showed CD69 expression in CD138+ T cells could significantly increase the apoptosis level of them. That indicated PI stimulation could induce specific apoptosis of CD138+ T cells via increasing CD69 expression in CD138+ T cells. In addition, our results showed CD138- T cells in MRL/lpr mice had significant defects in activation. However, to activate T cells could significantly prevent CD138 expression in CD3+ T cells of MRL/lpr mice. Our results suggested CD138 expression in CD3+ T cells of MRL/lpr mice was probably caused by the failure of activation in autoreactive T cells before self-antigens exposure to immune system.
Fuzzy set intersection based paired-end short-read alignment
Authors: Bolosky William , Subramaniyan Arun , Zaharia Matei , Pandya Ravi , Sittler Taylor , Patterson David 
Published: December 2021

Abstract
Much genomic data comes in the form of paired-end reads: two reads that represent genetic material with a small gap between. We present a new algorithm for aligning both reads in a pair simultaneously by fuzzily intersecting the sets of candidate alignment locations for each read. This algorithm is often much faster and produces alignments that result in variant calls having roughly the same concordance as the best competing aligners.
Electromotive Force Generated in All Materials under Temperature Difference
Authors: Song Dong-il 
Published: December 2021

Abstract
In this research, we investigate the thermoelectric effects of general materials. The results of this showed that an electromotive force was generated under a temperature difference between two points in materials. As no material has infinite electric resistance, an electromotive force is expected to be generated under a temperature difference in all materials. In conclusion, the thermoelectric effect generates an electromotive force. This electromotive force causes an electric current to flow, thereby generating a magnetic field.This magnetic field generates the Earth's magnetic field, triboelectricity, sunspots, and kinetic energy of celestial bodies.This temperature differential electromotive force also generates lightning and creates an ionosphere that reflects radio waves.
Leveraging Machine Learning To Assess Market-Level Food Safety And Zoonotic Disease Risks In China
Authors: Gao Qihua , Levi Retsef , Renegar Nicholas 
Published: December 2021

Abstract
While many have advocated for widespread closure of Chinese wet and wholesale markets due to numerous zoonotic disease outbreaks (e.g., SARS) and food safety risks, this is impractical due to their central role in China’s food system. This first-of-its-kind work offers a data science enabled approach to identify market-level risks. Using a massive, self-constructed dataset of food safety tests, market-level adulteration risk scores are created through machine learning techniques. Analysis shows that provinces with more high-risk markets also have more human cases of zoonotic flu, and specific markets associated with zoonotic disease have higher risk scores. Furthermore, it is shown that high-risk markets have management deficiencies (e.g., illegal wild animal sales), potentially indicating that increased and integrated regulation targeting high-risk markets could mitigate these risks.
Developing a Hazard Profile of The Kashmir Valley Through Historical Data Analysis For The Period 1900-2020
Authors: Ali Noureen , Alam Akhtar , Bhat M Sultan , Shah Bilquis 
Published: December 2021

Abstract
Disasters not only cause high mortality and suffering, but thwart developmental activities and damage local economies in process of formation. A part of the NW Himalayas, the Kashmir Valley is very distinct with respect to its location, topography, climate, socioeconomic structure, and strategic geopolitical nature owing to which it has witnessed a multitude of disasters ranging from local incidents of rockfalls to catastrophic earthquakes, and has often paid heavily in terms of loss of life and property. However, the information on most of the events is either partially reported or exaggerated or sometimes not recorded at all and largely scattered. Availability of organized and reliable record of past hazards and disasters is essential for tackling the risks and mitigating the future disasters. In this context, the present study attempts to address the lack of data availability by focusing on developing a dependable hazard and disaster catalogue of the Kashmir Valley by investigating into the existing literature and the available secondary data sources. A record of natural hazards and disasters most prevalent in the valley viz., earthquakes, floods, landslides and snow avalanches, has been compiled for the time period 1900 to 2020 by making use of various secondary sources, comprising of 1854 events with a range of triggers and impacts reported in the valley, which provide an insight into the spatial and temporal (frequency and distribution) trends of different hazard types for the selected time-period. Developing a catalogue of events reported in the Kashmir Valley can help in building a hazard and disaster scenario which serves as a reliable information source and is of great value from the perspective of regional design, planning and policy responses to promote disaster risk reduction.
Pulsed Magnetic Field Treatment of TiAlSiN Coated Milling Tools For Improved Cutting Performances
Authors: Qu Hao , Zhang Lin , Chen Zhe , Zhang Lei , Jiang Kyle , Liu Jian 
Published: December 2021

Abstract
In this study a pulsed magnetic treatment was attempted to improve the cutting performance of the TiAlSiN coated WC-12wt%Co cemented carbide end mills and the effects of the strength of the pulsed magnetic field on the cutting forces, the cutting vibrations, the tool wear, the machined surface roughness and mechanical properties were investigated. It is found that the cutting performances of the coated tools are successfully improved with a relatively lower cutting force and less wear area. The average resultant cutting force Fxyave decrease by 14.53% in the last machining process when the optimum processing parameters of 0.5T magnetic field is used, accompanying a maximum decrease of 46.8% in the cutting vibration. The maximum reductions of 57.65% and 25.4% in the flank wear and the average surface roughness of the workpiece are obtained respectively after the treatment. Both the hardness and toughness of the cemented carbides are slightly improved with the imposition of the field. The improvements in the cutting performance of the tool are attributed to the enhanced adhesion strength between the coating and matrix, which is caused by the increased compressive residual stress induced by the PMT.
Comparison of Laparoscope-Assisted Single-Needle Laparoscopic Percutaneous Extraperitoneal Closure versus Open Repair for Pediatric Inguinal Hernia
Authors: Wu Shaofeng , Xing Xiaoyu , He Rong , Zhao Haiteng , Zhong Liang , Sun Jie 
Published: December 2021

Abstract
Background:

Laparoscopic repairs have gained gradual acceptance in pediatric inguinal hernia over the past decade. However, consensus about the optimal management is still lacking. The aim of this study is to present a modified single-needle laparoscopic approach with the assistance of a laparoscope and to evaluate the surgical outcomes in comparison with traditional open repair method.
Materials and Methods:

We retrospectively reviewed the medical data of children who underwent laparoscope-assisted single-needle laparoscopic percutaneous extraperitoneal closure (LPEC) and open repair (OR) for inguinal hernia from 2014 to 2019. Data were reviewed and analyzed with respect to the operating time, the incidence of metachronous contralateral inguinal hernia (MCIH), recurrence, and other complications.
Results:

In our cohort, 961 patients in the OR group and 1098 patients in the LPEC group were analyzed retrospectively. Mean operative time was significantly shorter in the LPEC group (22.3±3.5 min) than in the OR group (27.8±5.9 min) for bilateral hernia repair (p<0.001). Iatrogenic cryptorchidism occurred statistically more frequently in the OR group than in the LPEC group (0.4% vs. 0%, p=0.013). In addition, the incidence of MCIH was 3.7% (33/887) in the OR group and 0.3% (3/1014) in the LPEC group (p<0.01).
Conclusion:

Based on the current outcomes, laparoscope-assisted single-needle LPEC provides a simple and attractive option for the administration of pediatric patients with inguinal hernia/hydrocele to reduce the MCIH.
New Perspective on Fatigue in Hemodialysis Patients With Preserved Ejection Fraction: Diastolic Dysfunction
Authors: Akbay Ertan , AKINCI Sinan , CONER Ali , ADAR Adem , GENCTOY Gultekin , DEMIR Ali Riza 
Published: December 2021

Abstract
Purpose: The relationship between diastolic dysfunction and fatigue in hemodialysis patients with preserved ejection fraction is unknown. In this context, the objective of this study is to assess fatigue using the relevant scales and to demonstrate its relationship with diastolic dysfunction.Methods: The patients who underwent hemodialysis were evaluated prospectively. Patients' fatigue was assessed using the Visual Analogue Scale to Evaluate Fatigue Severity (VAS-F). The echocardiographic works were performed as recommended in the American Society of Echocardiography guidelines.Results: A total of 94 patients [mean age 64.7±13.5 years, 54 males (57.4%)] were included in the study. The median VAS-F score of these patients was 68.5 (33.25-91.25), and they were divided into two groups according to this value. Peak myocardial velocities during early diastole (e') and tricuspid annular plane systolic excursion (TAPSE) values were found to be significantly lower in the group with high VAS-F scores, whereas the early diastolic flow velocities (E)/e' ratio and pulmonary artery peak systolic pressures (PAP) were found to be significantly higher (p<0.05, for all). E/e' ratio (r:0.311, p:0.002) and PAP (r:0.281, p:0.006) values were found to be positively correlated with the VAS-F score, as opposed to the TAPSE (r:-0.257, p:0.012) and e' (r:-0.303, p:0.003) values, which were found to be negatively correlated with the VAS-F score. ConclusionHigh fatigue scores in hemodialysis patients may be associated with diastolic dysfunction. In addition, in our study, we determined the correlation of VAS-F score with E/e' ratio, PAP and TAPSE.
A novel method to predict nonvisible symptoms using machine learning in cancer palliative care
Authors: Shimada Kazuki , Tsuneto Satoru 
Published: December 2021

Abstract
Patients with cancer at the end of life may find it difficult to express their symptoms if they can no longer communicate verbally because of deteriorating health. In this study, we assessed these symptoms using machine learning. We conducted a clinical survey of 213 cancer patients from August 2015 to August 2016. We divided the reported symptoms into two groups—visible and nonvisible symptoms. Our machine learning model used patient background data and visible symptoms to predict nonvisible symptoms: pain, dyspnea, fatigue, drowsiness, anxiety, delirium, inadequate informed consent, and spiritual issues. The highest and/or lowest values for prediction accuracy, sensitivity, and specificity, respectively, are as follows: 88.0%/55.5%, 84.9%/3·3%, and 96.7%/24.1%. This work will facilitate better assessment and management of symptoms in patients with cancer.
Influence of Pinealectomy and Long-term Melatonin Administration on Inflammation and Oxidative Stress in Experimental Gouty Arthritis
Authors: BALLUR Arwa Fadıl Haqi , ALTINOZ Eyup , YİGİTTURK Gurkan , Onal Melike Ozgul , ELBE Hulya , BICER Yasemin , KARAYAKALI Melike , demir mehmet 
Published: December 2021

Abstract
Gout is an inflammatory arthritis characterized by the deposition of monosodium urate (MSU) crystals in the joints or soft tissue. MSU crystals are potent inflammation inducers. Melatonin (MLT) is a powerful endogenous anti-inflammatory agent and effective in reducing cellular damage. In the present study, possible underlying mechanisms associated with anti-inflammatory and anti-oxidative effects were investigated in rats with gouty arthritis and melatonin deprivation treated with MLT. Fifty-six rats were divided into seven groups: control, sham control, pinealectomy (PNX), MSU (On the 30th day, single dose 20 mg/ml, intraperitoneal), MSU+MLT (10 mg/kg/day for 30 days, intraperitoneal), MSU+PINX and MSU+PINX+MLT. PNX procedure was performed on the first day of the study. As compared to the controls, the results showed that MSU administration caused significant increases in oxidative stress parameters (malondialdehyde and total oxidant status). Besides, significant decreases in antioxidant defense systems (glutathione, superoxide dismutase and total antioxidant status were observed. A statistically significant increase was found in the mean histopathological damage score in the groups that received MSU injection. It was found that histopathological changes were significantly reduced in the MSU+MLT group given MLT. In our study, it was determined that many histopathological changes, as well as swelling and temperature increase in the joint, which are markers of inflammation, were significantly reduced with MLT supplementation. These results suggest that melatonin ameliorates MSU-induced gout in the rat through inhibition of oxidative stress and proinflammatory cytokine production.
Metagenomic Characterization of The Tracheobronchial Microbiome in Lung Cancer
Authors: Hogue Stephanie , Pierce Christine M. , Paul Shirlene , Thapa Ram , Kim Youngchul , Robinson Lary A. 
Published: December 2021

Abstract
BackgroundThe tracheobronchial and oral microbiome may be associated with lung cancer, potentially acting as predictive biomarkers. Therefore, we studied the lung and oral bacteriome and virome in non-small cell lung cancer (NSCLC) patients compared to melanoma controls to discover distinguishable features.MethodsIn this pilot case-control study, we recruited ten patients with resectable NSCLC (cases) and ten age-matched melanoma patients (controls) who both underwent tumor resection. Preoperative oral gargles were collected from both groups, who then underwent transbronchoscopic tracheal lavage after intubation. Lung tumor and adjacent non-neoplastic lung were sterilely collected after resection. Microbial DNA from all specimens underwent 16S rRNA gene sequencing. Lavage and gargle specimens underwent whole-genome shotgun sequencing. Microbiome metrics were calculated to compare both cohorts. T-tests and Wilcoxon rank sum tests were used to test for significant differences in alpha diversity between cohorts. PERMANOVA was used to compare beta diversity. ResultsNo clear differences were found in the microbial community structure of case and control gargles, but beta diversity of case and control lavages significantly differed. Two species, Granulicatella adiacens and Neisseria subflava, appeared in higher abundance in case versus control lavages. Case lavages also maintained higher relative abundances of oral commensals compared to controls. ConclusionsLung lavages demonstrated oral microbiota enrichment in cases compared to controls, suggesting microaspiration and resultant inflammation. The oral commensals Granulicatella adiacens and Neisseria subflava were more abundant in the tracheobronchial lavages of lung cancer versus melanoma patients, implicating these microorganisms as potential lung cancer biomarkers, warranting further validation studies.