bluuebunny/crossref_metadata_2025_split huggingface dataset:
Next Generation Sequencing-Based Mitochondrial Genome Analysis In Relation To The Drug- Induced Toxicity In Korean Subjects
Authors: Hoa Pham Vinh , Lam Nguyen Van , Jung Hye-Eun , Cho Yong-Soon , Shin Jae-Gook 
Published: November 2021

Abstract
Background: Mitochondrial variants have been investigated to be associated with many diseases, which was reported largely from European populations. Few studies, however, have annotated the whole mitochondrial DNA (mtDNA) genome associated with drug responses including adverse drug reactions (ADRs), especially in Asian populations. This study was performed to characterize mtDNA genetic profiles, especially the distribution and frequency of well-known genetic biomarkers associated with diseases and drug-induced toxicity, in the Korean population by using high throughput next-generation sequencing.Results: A total of 681 variants was identified among all 118 Korean subjects. The MT-TRNP and displacement loop (D-loop) showed the highest numbers of variants (113 and 74 variants, respectively). In the D-loop, 25.4% of the subjects were identified to have m.16189T>C allele, which was known to reduce the mtDNA copy number variation in human cells. The variant m.1095T>C, annotated to aminoglycoside-induced ototoxicity, was found from only one subject in this study, while the frequency of m.2706A>G and m.3010A>G, which are associated with antibiotic-induced toxicity, were 99.15% and 30.51%, respectively. The 5 subjects were identified to have the variant m.2150T>TA, a genotype associated with highly disruptive effects on mitochondrial ribosomes. In addition, the mitochondrial haplogroups of 118 Korean subjects were found that D and M groups were the most dominant groups with the frequency of 34.74% and 16.1%, respectively. Conclusions: Our finding was constant with Korean 1K project and well reflected the unique profile of mitochondrial haplogroup distribution. It was the first study to annotate the whole mitochondrial genome with drug-induced toxicity to predict the ADRs event in clinical implementation for Korean population. This approach could be extended for further study for validation the potential ethnic specific mitochondrial genetic biomarkers in Korea population.
When will the world be committed to 1.5 and 2.0°C of global warming?
Authors: Dvorak Michelle , Armour Kyle , Frierson Dargan , Proistosescu Cristian , Baker Marcia , Smith Christopher 
Published: November 2021

Abstract
We investigate committed warming, i.e., the global mean temperature change that would follow complete cessation of anthropogenic emissions. The removal from the atmosphere of short-lived particulate aerosols, which have a cooling effect on the climate, leads to a peak in warming within a decade, followed by a slow decline over centuries to millennia to a relatively stable temperature determined by the residual CO2 forcing. This has important consequences: temporary warming well beyond present-day levels without any additional emissions. We use an emissions-based climate model (FaIR) to estimate temperature change after abrupt cessation of all anthropogenic emissions in 2021 and in every year thereafter until 2080, assuming that emissions prior to cessation proceed along priority Shared Socioeconomic Pathways (SSPs). We find that society may already be committed to peak warming of greater than 1.5°C with approximately 40% probability, with a small (2%) probability of peak warming greater than 2.0°C. The probability of being committed to 1.5°C increases to at least 50% by 2024. Taking into account short-lived climate forcers advances warming commitments by a half a decade, considerably reducing the remaining carbon budget. While an abrupt cessation of all anthropogenic emissions is not likely to occur, this idealized scenario provides a quantification of when we will be committed to exceeding key global warming levels while following realistic emissions scenarios.
Three-Dimensional Morphometry of Normal Japanese Knees For Designing Total Knee Arthroplasty
Authors: Tomita Tetsuya , Suzuki Masahiko , Nakamura Takashi , Ryu Keinosuke , Tsumura Hiroshi 
Published: November 2021

Abstract
Background: To examine racial and sex-related differences in bone morphology and to determine whether it is necessary to take sex-related and ethnic differences into account in designing artificial knee joints.Methods: Hundred Japanese women normal knees, 88 Japanese men normal knees, and 18 Caucasian women normal knees were examined. Knee joints were measured to enable selection and design of artificial knee joints based on assumed bone resection face dimensions in TKA. Results: The dimensional measurements were performed by reading the three-dimensional CT based bone model. F-MAP/F-ML and F-LAP/F-ML relative to F-ML dimensions and T-MAP/T-ML and T-LAP/T-ML relative to T-ML dimensions were determined in both sexes. Results: In both sexes and in both the femur and tibia, the value increased with decreasing size. Therefore, the width narrowed with decreasing size. It was considered that the width may be narrower in women than in men because women have smaller bone sizes than men. The matching ratio was considered to improve as the aspect ratio is adjusted according to the size when an artificial joint is designed. There were no significant differences in the measured sagittal flexion angle between the Japanese and Caucasian women. Conclusion: The individual differences were greater than the racial differences; therefore, we consider that there is no significant need to change the shape of the artificial knee joint according to racial differences in bone morphology.
Two Different Minimally Invasive Approaches for Management of Thoracolumbar Fractures
Authors: Yu Chenghao , Ding Fan , Wu Xiaosong , Ye Zhengyun , Hu Bing 
Published: November 2021

Abstract
Objective To compare the clinical effect and safety of pedicle screw fixation via percutaneous approach and Wiltse paraspinal approach for thoracolumbar fractures without neurological deficit.Methods 98 cases who suffered from single level thoracolumbar fracture without nerve injury were treated by pedicle screws fixation via either percutaneous approach (percutaneous group) and Wiltse paraspinal approach(paraspinal group). Perioperative indexes, imaging parameters and functional and symptom results of the two groups were recorded and compared. Results All patients were followed for more than 12 months, and the incision length and postoperative hospital stay in the percutaneous group were significantly shorter than those in the paraspinal group (P<0.05), intraoperative blood loss was less than that of the paraspinal group (P<0.05), operative and postoperative costs and the number of fluoroscopy were significantly higher than those of the paraspinal group (P<0.05). There was no significant difference in operative time between the two groups (P > 0.05). The anterior edge height percentage of the injured vertebrae and kyphosis Cobb Angle were significantly improved 1 week and 1 year postoperatively in each group (P<0.05), there was no statistical difference between the two groups (P<0.05). As for Visual Analog Scale (VAS) scores, in each group there were continuous decreases 3 days, 6 months, and 1 year postoperatively (P<0.05); There were no statistically significant differences between the two groups before operation, 6 months and 1 year postoperatively (P<0.05), but a significant difference 3 days postoperatively (P<0.05). In terms of Oswestry disability index (ODI), in each group there was continuous decreases 6 months and 1 year postoperatively (P<0.05); and there was no significant difference between the two groups (P<0.05).There was no significant difference in the accuracy of implant between the two groups (P<0.05). In the percutaneous group, there were 2 cases of incision fat liquefaction, 1 case of guidewire fracture and 1 case of the anterior wall of the vertebra penetrated by guide wire rupture. 1 diabetic case of superficial incision infection and 2 cases of skin edge necrosis were found in the paraspinal group.Conclusion In the treatment of thoracolumbar fractures without neurological defect, pedicle screw fixations via Wiltse paraspinal and percutaneous approach both can obtain minimally invasive and reliable effect, but the percutaneous approach bring smaller trauma, less blood loss, longer operation time, more fluroscopy, higher surgery and postoperative costs, with its own unique complications especially in early learning curve.
The Comparation of Two Instruments for Rat Cervical 2 Hemisection Model
Authors: Shen LinLin , Song Chen , Zhang Liang , Wang Kai 
Published: November 2021

Abstract
The lateral C2 hemisection (HS) rat is the most studied reclinical model in the study of respiratory function after high cervical spinal cord injury. There are two main surgical methods in several studies— microscissors or microscalpel. This study is to evaluate the experimental results between those two methods. In this study, we performed rat lateral C2HS by microscissors (group A) or microscalpel (group B). We record cut frequency during hemisection as well as recovery of diaphragm electrophysiology by electromyogram (EMG) on the 14th day post injury. On the 14th day post injury, we record survival rate and evaluated the injury extent by hematoxylin-eosin (HE) stain. As a result, we found that group A had milder C2 injury extent than group B, higher survival rate on the 14th day post injury, and higher percent of peak root mean square (RMS) EMG post injury to that before injury. However, group A had larger cut frequency during hemisection. Weigh the advantages and disadvantages, microscissors seem had superiority over microscalpel.
Asparagine-EDTA MNPs: A Highly Efficient And Recyclable Magnetic Multifunctional Core-Shell Nanocatalyst For Green Synthesis of Biologically-Active 3,4-Dihydropyrimidin-2(1H)-One Compounds
Authors: Rostami Negin , Dekamin Mohammad G , Valiey Ehsan , FaniMoghadam Hamidreza 
Published: November 2021

Abstract
In this study, the new asparagine grafted on the EDTA-modified Fe3O4@SiO2 core-shell (Fe3O4@SiO2-APTS-EDTA-asparagine) magnetic nanoparticles were prepared and their structures were properly confirmed using different spectroscopic, microscopic and magnetic methods or techniques such as FT-IR, EDX, XRD, FESEM, TEM, TGA and VSM. The Fe3O4@SiO2-APTS-EDTA-asparagine core-shell nanomaterial was examined, as a highly efficient multifunctional and recoverable nanocatalyst, for the synthesis of a wide range of nitrogen-containing heterocycles and biologically-active 3,4-dihydropyrimidin-2(1H)-one derivatives under solvent-free conditions. It was proved that Fe3O4@SiO2-APTS-EDTA-asparagine MNPs, as a catalyst having excellent thermally and magnetic stability, specific morphology and acidic sites, can activate the Biginelli reaction components. Moreover, environmental-friendliness and nontoxic nature properties of the catalyst, cost effectiveness, low catalyst loading, easy separation of the catalyst from products and short time of reaction are some of the remarkable advantages of this green protocol.
Metastasis suppressor 1 controls osteoblast differentiation and bone homeostasis through regulating Src-Wnt/β-catenin signaling
Authors: Chen Meng , Shan Liying , Gan Ying , Tian Lijie , Zhou Jie , Zhu Endong , Yuan Hairui , Li Xiaoxia , Wang Baoli 
Published: November 2021

Abstract
Metastasis suppressor 1 (MTSS1) plays an inhibitory role in tumorigenesis and metastasis of a variety of cancers. To date, the function of MTSS1 in the differentiation of marrow stromal progenitor cells is completely unknown. In the current study, we explored whether and how MTSS1 has a role in osteoblast differentiation and bone homeostasis. Our data showed that MTSS1 mRNA was upregulated during osteoblast differentiation and downregulated in the osteoblastic lineage cells of ovariectomized and aged mice. Functional studies revealed that MTSS1 promoted the osteogenic differentiation from marrow stromal progenitor cells. Mechanistic explorations uncovered that the inactivation of Src and afterwards activation of canonical Wnt signaling were involved in osteoblast differentiation induced by MTSS1. The enhanced osteogenic differentiation induced by MTSS1 overexpression was attenuated when Src was simultaneously overexpressed, and conversely, the inhibition of osteogenic differentiation by MTSS1 siRNA was rescued when the Src inhibitor was supplemented to the culture. Finally, the in vivo transfection of MTSS1 siRNA to the marrow of mice significantly reduced the trabecular bone mass, along with the reduction of trabecular osteoblasts, the accumulation of marrow adipocytes, and the increase of phospho-Src positive cells on the trabeculae. No change in the number of osteoclasts was observed. This study has for the first time unraveled that MTSS1 contributes to osteoblast differentiation and bone homeostasis through regulating Src-Wnt/β-catenin signaling. It also suggests the potential of MTSS1 as a new target for the treatment of osteoporosis.
A Study on the Association Between Subclinical Hypothyroidism and Polycystic Ovary Syndrome
Authors: Wang mei , Li Xiaofeng , Ding Nan , Wang Fang 
Published: November 2021

Abstract
Background: Polycystic ovary syndrome (PCOS) and hypothyroidism are endocrine and metabolic disorders. Hypothyroidism has been found to be related to changes in blood lipids and insulin insensitivity. However, the relationship between subclinical hypothyroidism (SCH) and endocrine disorders in PCOS patients remains unclear. The incidence of SCH in PCOS patients is increasing. Metabolomics methods have been used to investigate the differences in metabolites and metabolic pathways between normal body weight and overweight (not obese) PCOS patients with SCH. Understanding the association between PCOS and SCH can guide diagnosis and treatment.Methods: We performed an untargeted serum metabolomics analysis in 62 PCOS patients. From 38 PCOS patients with SCH, 24 were selected and divided into the overweight (n = 13) and normal weight (n = 11) groups. Differential metabolites were identified using ultra-high-performance liquid chromatography–quadrupole time-of-flight mass spectrometry analysis. The significance of metabolites was evaluated by calculating the variable importance in projection score (> 1 and P < 0.01) from partial least squares discriminant analysis (PLS-DA) and orthogonal PLS-DA models. Kyoto Encyclopedia of Genes and Genomes pathway analysis was conducted to investigate the metabolomic pathways. P < 0.05 (Fisher’s exact test) was considered statistically significant.Results: In PCOS patients with SCH, significant differences in body weight, right ovary volume, homeostasis model assessment for insulin resistance value, insulin level at 2 h after a meal, and triglyceride level were observed between the overweight and normal weight groups. Twenty-six different metabolites were identified, mainly fatty acids and phosphatidylcholines, to have significantly levels in overweight patients with SCH. Moreover, 18 enriched metabolic pathways were identified, mainly biosynthesis of fatty acids and unsaturated fatty acids, digestion and absorption of proteins, aminoacyl-tRNA biosynthesis, and ATP-binding cassette (ABC) transporters.Conclusion: The interaction between body mass index and thyroid-stimulating hormone affects the metabolic status of PCOS patients. Overweight PCOS patients with SCH may have the worst metabolic status. The overweight and normal weight groups showed differences in glycerol phospholipid, sterol lipid, phosphatidylcholine, and androsterone sulphate levels. PCOS with SCH affects endocrine metabolism and ester metabolism through fatty acid biosynthesis, protein digestion and absorption, and ABC transporters.
A Beam Broadening Method for Phased Arrays in Wireless Communications
Authors: Fonteneau Corentin , Crussière Matthieu , Jahan Bruno 
Published: November 2021

Abstract
5G and IEEE 802.11ay introduce the use of the millimeter band as one promising solution to provide broadband wireless communication at multi-Gb/s user data rate. Due to the severe path-loss at such frequencies, it is generally assumed that large antenna arrays are used at the base station to steer narrow beams and build highly directionnal communication links towards the terminal points. However, broader and less directional beams are also of high interest in some of the steps involved in the establishment or the maintenance of the communication links. Indeed, search of a large area by narrow beams becomes too time consuming and link outage becomes more critical, thus affecting the latency and the robustness of the communications. A method enabling an adaptation of the beam widths is then worthwhile to consider.
In this article, we investigate how narrow beams naturally produced by large antenna arrays can be broadened to adapt the beam width to a desired angular sector. We consider that the multi-antenna processing is performed by phase shifters on the radio-frequency stage since its digital counterpart is hardly feasible in practice at such high frequencies. The main idea of our systematic phase-only beam broadening technique relies on the determination of a quadratic phase excitation law from a desired beam width and steering angle. We first lead a thorough analysis of the radiation behavior regarding the coefficients of such quadratic excitation. We then propose a calculation method for determining the polynomial coefficients as a function of the desired beam width and steering angle. This non-iterative beam broadening method is described for boresight and non-boresight directions and is intended for discrete antenna arrays.
A Six-Immune-Related Genes Prognostic Signature for Glioblastoma
Authors: Huang Kate , Rao Changjun , Lu Jianglong , Zhu Zhangzhang , Wang Chengde , Tu Ming , Sheng Chaodong , Zheng Shuizhi , Chen Xiaofang , Li Qun 
Published: November 2021

Abstract
Background: Glioblastoma (GBM) multiforme is a common malignant brain tumor with high mortality. It is urgently necessary to develop a new treatment because traditional approaches have reached a bottleneck.Purpose: Here we created an immune-related gene (IRGs)-based prognostic signature to comprehensively define the prognosis of glioblastoma (GBM).Methods: Glioblastoma samples were abstracted from the Chinese Glioma Genome Atlas (CGGA) and the Gene Expression Omnibus (GEO). We retrieved IRGs from the ImmProt data resource. Univariate Cox analysis was adopted to determine the prognostically remarkable IRGs for individual with GBM. The prognostically optimal IRGs were determined via LASSO regression, and predictive model created. Besides, the association of specific factors with the overall survival (OS) of individuals with GBM was explored via multivariate Cox-regression. Lastly, we constructed a predictive nomogram integrating the independent predictive factors to determine the one-, two-, and three-year OS likelihoods of individuals with GBM. Additionally,gene set enrichment analysis(GSEA) and single sample GSEA(ssGSEA) were performed to understand the correlation between the risk score and immune activity.Results: Overall, 273 IRGs which exhibited differential expression were identified in GBM tumor in contrast with the non-malignant samples. Of these 273 IRGs, only six were remarkably linked to OS of individuals with GBM, which were employed in constructing the predictive signature. The GBM were categorized into either the high-risk GBM group or the low-risk GBM group. There were remarkable differences between the high-risk GBM and the low-risk GBM groups regarding OS. The AUC for predicting one-,two-, and three-year OS in training set was 0.610,0.698 and 0.694.In line with the AUC of validation set was 0.608,0.692 and 0.678.Besides,the results of ssGSEA showed the score of prognostic signature is closely related to immune activity.Conclusion: Herein, a robust predictive model based on IRGs was created to estimate the diversity of OS likelihoods in GBM patients, as well as aid future clinical research.
Sacral Osteotomy Combined With Lumbopelvic Distraction Osteosynthesis in the Treatment of Malunion or Nonunion of Vertically Displaced Pelvic Fractures
Authors: Luo Yangxing , He Li , Li Yue , Xie Jie , Gong Song , Zhang Qian , Yin Enzhi , Gu Meiqi , Yi Chengla 
Published: November 2021

Abstract
Background Malunion or nonunion of vertically displaced pelvic fractures resulting in lower limb length discrepancies, claudication, and pain. There have been few previous reports of this type of corrective surgery of the old pelvis. We present a new surgical technique of sacral osteotomy combined with lumbopelvic distraction osteosynthesis (LPDO) in the treatment of malunion and nonunion of vertically displaced pelvic fractures and report on its short-term clinical results.Methods We retrospectively reviewed nine patients (five males and four females) with malunion or nonunion of vertically displaced pelvic fractures treated with sacral osteotomy and LPDO from April 2015 to January 2020. The age ranged from 14 to 45 years (average, 30.7 years). The time from injury to deformity correction surgery ranged from 3 months to 5 years (average, 12.8 months). The vertical displacement of a unilateral pelvis was 3.0-4.5 cm (average, 3.80 cm). According to the AO/OTA classification at initial pelvic fracture, eight cases were type C1.3 and one case were type C3.3. Sacral osteotomy and LPDO were used in all nine patients. The degree of unilateral pelvic reduction was assessed postoperatively based on measurements from the anteroposterior (AP) X-ray. The Majeed score and pain visual analog scale (VAS) score were used to assess the therapeutic effect of the patients during follow-up.Results In all nine patients, postoperative AP X-ray showed correction displacement of 1.7-3.9 cm (average, 3.20 cm). All the patients were followed up for 6 to 36 months (average, 12.7 months). At the last follow-up, the Majeed score of pelvic fracture increased from an average of 53.9 points (30-84 points) preoperatively to 87.0 points (72-94 points), and the VAS score for pain decreased from an average of 6.0 points (4-8 points) preoperatively to 1.2 points (0-3 points). None of the patients had complications, such as infection, implant broken, screw loosening, iatrogenic nerve, or blood vessel injury. Conclusion Sacral osteotomy combined with LPDO for the treatment of pelvic malunion or nonunion caused by sacral fracture can correct significantly vertical displacement of a unilateral pelvis, prolong limb length, and reconstruct the stability of a pelvic ring, leading to good clinical results.