bluuebunny/crossref_metadata_2025_split huggingface dataset:
Nucleobase clustering contributes to the formation and hollowing of repeat-expansion RNA condensate
Authors: Ma Ying-Xue , Li Hao-Zheng , Gong Zhou , Yang Shuai , Wang Ping , Tang Chun 
Published: November 2021

AbstractRNA molecules with repeat expansion sequences can phase separate into gel-like condensate, and this process may lead to neurodegenerative diseases. Here we report that in the presence of Mg2+ion, RNA molecules containing 20×CAG repeats coacervate into filled droplets or hollowed condensate. Using hyperspectral stimulated Raman spectroscopy, we show that RNA coacervation is accompanied by the stacking and clustering of nucleobases, while forfeiting the canonical base-paired structure. At an increasing RNA/Mg2+ratio, the RNA droplets first expand in sizes, and then shrink and adopt hollow vesicle-like structures. Significantly, for both large and vesicle-like droplets, the nucleobase-clustered structure is more prominent at the rim than at the center, accounting for the rigidification of RNA droplets. Thus, our finding has broad implications for the general aging processes of RNA-containing membrane-less organelles.
TRIM24 controls induction of latent HIV-1 by stimulating transcriptional elongation
Authors: Horvath Riley M. , Dahabieh Matthew , Malcolm Tom , Sadowski Ivan 
Published: November 2021

AbstractThe conserved HIV-1 LTR cis elements RBE1/3 bind the factor RBF2, consisting of USF1/2 and TFII-I, and are essential for reactivation of HIV-1 by T cell signaling. We determined that TFII-I recruits the tripartite motif protein TRIM24 to the LTR, and this interaction is required for efficient reactivation of HIV-1 expression in response to T cell signaling, similar to the effect of TFII-I depletion. Knockout of TRIM24 did not affect recruitment of RNA Pol II to the LTR promoter, but inhibited transcriptional elongation, an effect that was associated with decreased RNA Pol II CTD S2 phosphorylation and impaired recruitment of CDK9 to the LTR. These results demonstrate that TFII-I promotes transcriptional elongation in response to T cell activation through recruitment of the co-factor TRIM24, which is necessary for efficient recruitment of the elongation factor P-TEFb.
Dosage of duplicated and antifunctionalized homeobox proteins influences spikelet development in barley
Authors: Thirulogachandar Venkatasubbu , Govind Geetha , Hensel Götz , Kale Sandip M. , Kuhlmann Markus , Eschen-Lippold Lennart , Rutten Twan , Koppolu Ravi , Rajaraman Jeyaraman , Palakolanu Sudhakar Reddy , Seiler Christiane , Sakuma Shun , Jayakodi Murukarthick , Lee Justin , Kumlehn Jochen , Komatsuda Takao , Schnurbusch Thorsten , Sreenivasulu Nese 
Published: November 2021

AbstractIlluminating the mechanisms of inflorescence architecture of grain crops that feed our world may strengthen the goal towards sustainable agriculture. Lateral spikelet development of barley (Hordeum vulgare L.) is such an example of a floral architectural trait regulated by VRS1 (Vulgare Row-type Spike 1 or Six-rowed Spike 1, syn. HvHOX1). Its lateral spikelet-specific expression and the quantitative nature of suppressing spikelet development were previously shown in barley. However, the mechanistic function of this gene and its paralog HvHOX2 on spikelet development is still fragmentary.Here, we show that these duplicated transcription factors (TFs) have contrasting nucleotide diversity in various barley genotypes and several Hordeum species. Despite this difference, both proteins retain their basic properties of the homeodomain leucine zipper class I family of TFs. During spikelet development, these genes exhibit similar spatiotemporal expression patterns yet with anticyclic expression levels. A gene co-expression network analysis suggested that both have an ancestral relationship but their functions appear antagonistic to each other, i.e., HvHOX1 suppresses whereas HvHOX2 rather promotes spikelet development. Our transgenic promoter-swap analysis showed that HvHOX2 can restore suppressed lateral spikelets when expression levels are increased; however, at its low endogenous expression level, HvHOX2 appears dispensable for spikelet development. Collectively, this study proposes that the dosage of the two antagonistic TFs, HvHOX1 and HvHOX2, influence spikelet development in barley.
Automated BIM schedule generation approach for solving time–cost trade-off problems
Authors: ElMenshawy Mohamed , Marzouk Mohamed 
Published: November 2021

PurposeNowadays, building information modeling (BIM) represents an evolution in the architecture, engineering and construction (AEC) industries with its various applications. BIM is capable to store huge amounts of information related to buildings which can be leveraged in several areas such as quantity takeoff, scheduling, sustainability and facility management. The main objective of this research is to establish a model for automated schedule generation using BIM and to solve the time–cost trade-off problem (TCTP) resulting from the various scenarios offered to the user.Design/methodology/approachA model is developed to use the quantities exported from a BIM platform, then generate construction activities, calculate the duration of each activity and finally the logic/sequence is applied in order to link the activities together. Then, multiobjective optimization is performed using nondominated sorting genetic algorithm (NSGA-II) in order to provide the most feasible solutions considering project duration and cost. The researchers opted NSGA-II because it is one of the well-known and credible algorithms that have been used in many applications, and its performances were tested in several comparative studies.FindingsThe proposed model is capable to select the near-optimum scenario for the project and export it to Primavera software. A case study is worked to demonstrate the use of the proposed model and illustrate its main features.Originality/valueThe proposed model can provide a simple and user-friendly model for automated schedule generation of construction projects. In addition, opportunities related to the interface between an automated schedule generation model and Primavera software are enabled as Primavera is one of the most popular and common schedule software solutions in the construction industry. Furthermore, it allows importing data from MS Excel, which is used to store activities data in the different scenarios. In addition, there are numerous solutions, each one corresponds to a certain duration and cost according to the performance factor which often reflects the number of crews assigned to the activity and/or construction method.
National Dairy deriving value through project management
Authors: Bhoola Vanita , Dwivedi Vineeta , Vedpuriswar Ayalur 
Published: November 2021

Subject area
Project Management, Entrepreneurship, Risk Management, Communication.

Study level/applicability
Study level: MBA and Executive Education.
Applicability: This case can be taught in the project management course. It will cover the project-planning phase, which is an iterative or progressive elaboration for the entire project lifecycle. The case will help in discussing how project management is an important discipline to manage projects and stakeholders effectively.

Case overview
The case depicts the dilemma of a passionate entrepreneur who is setting up an ambitious dairy business but project execution goes awry. The case discusses the challenges related to project planning and execution.
It captures the essence of proactive risk management, measures that can mitigate risks and create opportunities. The case also discusses the entire project lifecycle from project initiation to closure and the challenges a manager has to face in terms of stakeholder engagement, risk management, stakeholder communication and scope change.

Expected learning outcomes
To understand the alignment of the project with the organisation strategy; to learn to create a project plan and monitor and control the scope, schedule, resources and costs; to accurately estimate project costs, timelines, and quality and schedule, using tools like MS Project; to learn the risk management techniques for managing projects, teams and stakeholders; and to manage stakeholder communication effectively.

Supplementary materials
Teaching notes are available for educators only.

Subject code
CSS 2: Built Environment.
Technological regimes or “perceived” technological regimes? Evidence from the Italian wine industry
Authors: Muscio Alessandro , Nardone Gianluca , Stasi Antonio 
Published: November 2021

PurposeTechnological regimes define the environment in which innovative and learning activities take place in each sector of the economy. In this paper, the authors argue that technological regimes must be interpreted and elaborated by each organisation operating within a sector in order to be implemented rationally, which leads us to the concept of perceived technological regimes.Design/methodology/approachThe authors tested the relevance of firms' perceptions of different technological regimes on a sample of wine companies in Italy. The authors run a questionnaire survey and obtained 334 clean responses. Data drawn from questionnaires were analysed via econometric analysis.FindingsThe authors present empirical evidence that this perception tends to vary across different wine technologies. Additionally, the authors find evidence that firms' technology adoption, absorptive capacity and external knowledge sourcing have a strong impact on their perceptions of the relevance of a given wine-making technology.Originality/valueWhile individual technological regimes are characterised by systematic differences in the distribution of heterogeneous firm types, previous empirical studies have not explored whether the technological environment defining a given industry is differently interpreted and elaborated by each firm operating in it.
Integrated SDM database: Enhancing the relevance and utility of species distribution models in conservation management
Authors: Frans Veronica F. , Augé Amélie A. , Fyfe Jim , Zhang Yuqian , McNally Nathan , Edelhoff Hendrik , Balkenhol Niko , Engler Jan O. 
Published: November 2021

Abstract

Species' ranges are changing at accelerating rates. Species distribution models (SDMs) are powerful tools that help rangers and decision‐makers prepare for reintroductions, range shifts, reductions and/or expansions by predicting habitat suitability across landscapes. Yet, range‐expanding or ‐shifting species in particular face other challenges that traditional SDM procedures cannot quantify, due to large differences between a species' currently occupied range and potential future range. The realism of SDMs is thus lost and not as useful for conservation management in practice. Here, we address these challenges with an extended assessment of habitat suitability through an integrated SDM database (iSDMdb).

The iSDMdb is a spatial database of predicted sites in a species' prediction range, derived from SDM results, and is a single spatial feature that contains additional, user‐friendly data fields that synthesise and summarise SDM predictions and uncertainty, human impacts, restoration features, novel preferences in novel spaces and management priorities. To illustrate its utility, we used the endangered New Zealand sea lion Phocarctos hookeri. We consulted with wildlife rangers, decision‐makers and sea lion experts to supplement SDM predictions with additional, more realistic and applicable information for management.

Almost half the data fields included in this database resulted from engaging with these end‐users during our study. The SDM found 395 predicted sites. However, the iSDMdb's additional assessments showed that the actual suitability of most sites (90%) was questionable due to human impacts. >50% of sites contained unnatural barriers (fences, grazing grasslands), and 75% of sites had roads located within the species' range of inland movement. Just 5% of the predicted sites were mostly (>80%) protected.

Integrating SDM results with supplemental assessments provides a way to address SDM limitations, especially for range‐expanding or ‐shifting species. SDM products for conservation applications have been critiqued for lacking transparency and interpretation support, and ineffectively communicating uncertainty. The iSDMdb addresses these issues and enhances the practical relevance and utility of SDMs for stakeholders, rangers and decision‐makers. We exemplify how to build an iSDMdb using open‐source tools, and how to make diverse, complex assessments more accessible for end‐users.
Laparoscopic approach as a safe and effective option for incarcerated femoral hernias
Authors: Okazaki Ryo , Poudel Saseem , Hane Yuma , Saito Takahiro , Muto Jun , Syoji Yasuhito , Hase Ryunosuke , Senmaru Naoto , Hirano Satoshi 
Published: November 2021

AbstractIntroductionThe laparoscopic approach for elective femoral herniorrhaphy is well established. However, femoral hernias often present as incarcerations and require emergency repair surgery, mainly using the open approach. This study aimed to retrospectively analyze the efficacy of the laparoscopic approach for incarcerated femoral hernias.MethodsData of patients who underwent emergency surgery for incarcerated femoral hernia between April 2016 and August 2021 were retrospectively analyzed. Laparoscopy was performed whenever possible; however, conversion to an open approach remained a fallback option for when laparoscopic repair was not possible. In laparoscopic repair, incarcerated femoral hernias reduced using traction, water pressure, and preperitoneal methods. Data of patients who underwent open repair and laparoscopy were then compared.ResultsDuring the observation period, 20 patients underwent emergency surgery for incarcerated femoral hernia. Eleven patients subsequently underwent repair using a laparoscopic approach, and eight underwent repair using an open approach. Only one patient underwent intestinal resection without hernia repair due to perforated bowel. Operative time for laparoscopic repair was longer. Mesh repair was performed in 18 patients. Four patients each in the laparoscopic repair and open group required intestinal resection.ConclusionIncarcerated femoral hernias can be safely repaired using the laparoscopic approach.
Ethnic identity concealment and disclosure: Contexts and strategies
Authors: Dobai Anna , Hopkins Nick 
Published: November 2021

AbstractEthnic minority group members’ responses to their prejudicial treatment can take several forms. One involves identity concealment (e.g., ‘passing’). In order to understand such a response, we must explore participants’ understandings of the interactional context before them, their meta‐perceptions of the identity others ascribe to them, and the varied meanings that identity concealment/non‐disclosure may have in that context. Our analysis of interview data (N = 30) obtained with Roma in Hungary reveals diverse forms of, and motivations for, the concealment of their Roma identity. Some participants reported examples of proactive identity concealment, others reported more reactive forms (in which they went along with others’ mistaken assumptions concerning their identity). The motivations for identity concealment (whether proactive or reactive) included the desire to: secure material benefits; avoid conflict; take pleasure from seeing others’ assumptions blinding them to the reality before them; test (and expose) majority group members’ attitudes; allow themselves opportunities to experience the world in new ways. Our analysis highlights the importance of social identity researchers recognizing the diverse motivations for ethnic identity concealment: From the actors’ perspective concealment is not always assimilatory, and in some contexts can be experienced as empowering.
The impact of rent control: Investigations on historical data in the city of Lyon
Authors: Bonneval Loïc , Goffette‐Nagot Florence , Zhao Zhejin 
Published: November 2021

AbstractThis paper reexamines the debated issue of the effects of rent control policy on the rental market. We investigate the impact on rents of three different forms of rent regulation in Lyon over a 78‐year period. We use an original historical data set which allows us to track regulation changes, rent paid, and tenant moves for a long‐run panel of flats. Using a difference‐in‐differences method, we estimate the impact of regulation on rents depending on the type of rent control over different economic periods. Our results show that the impact of rent control deepened over time. Starting with an 11% reduction in rents between 1914 and 1929, it reached a decrease by 47% in the regulated rental market in the 1949–1968 period. We do not find any increase in rents in the unregulated segment of the rental market, which could be a result of a reduction in housing investment in the long run.
Perspectives of substitute decision‐makers and staff about person‐centred physical activity in long‐term care
Authors: Chu Charlene H. , Quan Amanda M. L. , Gandhi Freya , McGilton Katherine S. 
Published: November 2021

AbstractIntroductionThis paper aims to explore the care processes that best exemplify person‐centred care during physical activity (PA) for long‐term care (LTC) residents with dementia from the perspectives of substitute decision‐makers (SDMs) and LTC home staff. Little is known about how person‐centred care is reflected during PA for residents with dementia, or the barriers and benefits to enacting person‐centred care during PA.MethodsSemistructured interviews were used to collect SDMs and LTC home staffs' perspectives on the importance of person‐centred care during PA from two LTC homes in Canada. The McCormack and McCance person‐centredness framework was used to guide thematic content analysis of responses.ResultsSDM (n = 26) and staff (n = 21) identified actions categorized under the sympathetic presence or engagement care processes from the person‐centredness framework as most reflecting person‐centred care. Benefits of person‐centred care during PA were categorized into three themes: functional and physical, behavioural and communication and psychosocial improvements. Barriers to person‐centred care during PA identified were lack of time, opportunities for meaningful activity in LTC setting and staff experiences with resident aggression.SignificanceUnderstanding the care processes that are most recognized as person‐centred care and valued by SDMs and LTC home staff has implications for education and training. Insights into SDMs' care expectations regarding person‐centred care can inform staff about which actions should be prioritized to meet care expectations and can foster relationships to the benefit of residents with dementia.Patient and Public ContributionStudy participants were not involved in the development of research questions, research design or outcome measures of this study.
Clinical, radiological and pathological features of idiopathic and secondary interstitial pneumonia with pleuroparenchymal fibroelastosis in patients undergoing lung transplantation
Authors: Ikegami Naoya , Nakajima Naoki , Yoshizawa Akihiko , Handa Tomohiro , Chen‐Yoshikawa Toyofumi , Kubo Takeshi , Tanizawa Kiminobu , Ohsumi Akihiro , Yamada Yoshito , Hamaji Masatsugu , Nakajima Daisuke , Yutaka Yojiro , Tanaka Satona , Watanabe Kizuku , Nakatsuka Yoshinari , Murase Yuko , Nakanishi Tomoko , Niwamoto Takafumi , Chin Kazuo , Date Hiroshi , Hirai Toyohiro 
Published: November 2021

AimsIdiopathic pleuroparenchymal fibroelastosis (PPFE) is a rare type of idiopathic interstitial pneumonia, and pathological PPFE is also observed in patients with secondary interstitial pneumonia. This study aimed to evaluate the pathological findings associated with radiological PPFE‐like lesions and the clinical and morphological features of patients with pathological PPFE.Methods and resultsWe retrospectively reviewed the pathology of the explanted lungs from 59 lung transplant recipients with radiological PPFE‐like lesions. Pathological PPFE lesions were identified in 14 patients with idiopathic disease and in 12 patients with secondary disease. Pathological PPFE was associated with previous pneumothorax, volume loss in the upper lobes, and a flattened chest. Patients with idiopathic disease and those with secondary disease with pathological PPFE had similar clinical, radiological and pathological findings, whereas fibroblastic foci were more common in patients with idiopathic disease, and patients with secondary disease more frequently showed alveolar septal thickening with elastosis or fibrosis. Post‐transplantation survival did not differ between patients with idiopathic and secondary disease with pathological PPFE (log‐rank; P = 0.57) and was similar between patients with idiopathic disease with pathological PPFE and those with idiopathic pulmonary fibrosis (IPF) (log‐rank; P = 0.62).ConclusionsNot all patients with interstitial pneumonia with radiological PPFE‐like lesions have pathological PPFE. Characteristic clinical features can suggest the presence of pathological PPFE, and idiopathic and secondary cases with pathological PPFE are similar except for fibroblastic foci in idiopathic cases and alveolar septal thickening with elastosis or fibrosis in secondary cases. Patients with pathological PPFE have a similar prognosis to those with IPF after transplantation.
Using eye‐movements to assess underlying factors in online purchasing behaviors
Authors: Johnson Rebecca L. , Nambiar Devika , Suman Gabriella 
Published: November 2021

AbstractThe field of consumer neuroscience allows researchers to account for an individual’s explicit reported behaviors as well as their implicit behaviors that are reflected in the neural mechanisms that occur during the purchase decision phase of a consumer’s online shopping experience. The purpose of the current study was to use eye‐tracking technology in conjunction with self‐report purchase intention data to observe the relative impact of star rating, price, discount, and time pressure on purchase decisions. The results suggest that purchase intention was most affected by star rating, price, and discount with higher purchase intentions on items with higher star ratings, lower prices, and greater discounts. The eye movement data revealed that these factors, as well as time pressure, influenced where consumers directed their attention in making their purchasing decisions. These findings have significant implications for future ecommerce marketing strategy, especially across efforts to increase purchase intention.
Synthesis of yttria nanopowder with poly acrylic acid as dispersant for highly transparent yttria ceramics
Authors: Ni Meng , Wang Jun , Ma Jie , Yao Bingqing , Ong Boonchong , Dong Zhili , Tang Dingyuan 
Published: November 2021

AbstractHighly pure and well‐dispersed yttria nanocrystalline powders were synthesized with a modified coprecipitation method that uses mixed ammonium hydroxide‐carbonate solution as the precipitant and poly acrylic acid (PAA) as the dispersant. Comparing with the yttria powders synthesized using the same route but without PAA dispersant, the PAA‐assisted powders exhibit lower level of agglomeration and better sinterability. Sintering trajectories of the powders with and without PAA dispersant were investigated. Y2O3 ceramics sintered with the PAA‐assisted powders exhibited smaller grain size, homogeneous microstructure, and higher in‐line optical transmittance. The result suggests that the polymer‐assisted coprecipitation could be used to synthesize yttria nanopowders for fabricating high optical quality transparent ceramics.