bluuebunny/crossref_metadata_2025_split huggingface dataset:
Reflections on the Clinician’s Role with Individuals Who Self-identify as Transgender
Authors: Levine Stephen B. 
Published: September 2021

AbstractThe fact that modern patterns of the treatment of trans individuals are not based on controlled or long-term comprehensive follow-up studies has allowed many ethical tensions to persist. These have been intensifying as the numbers of adolescent girls declare themselves to be trans, have gender dysphoria, or are “boys.” This essay aims to assist clinicians in their initial approach to trans patients of any age. Gender identity is only one aspect of an individual’s multifaceted identity. The contributions to the passionate positions in the trans culture debate are discussed along with the controversy over the official, not falsifiable, position that all gender identities are inherently normal. The essay posits that it is relevant and ethical to investigate the forces that may have propelled an individual to create and announce a new identity. Some of these biological, social, and psychological forces are enumerated. Using the adolescent patient as an example, a model for a comprehensive evaluation process and its goals are provided. The essay is framed within a developmental perspective.
Multiomic Analyses of Dopaminergic Neurons Isolated from Human Substantia Nigra in Parkinson’s Disease: A Descriptive and Exploratory Study
Authors: Zaccaria Affif , Antinori Paola , Licker Virginie , Kövari Enikö , Lobrinus Johannes A. , Burkhard Pierre R. 
Published: September 2021

AbstractDopaminergic neurons (DA) of the substantia nigra pars compacta (SNpc) selectively and progressively degenerate in Parkinson’s disease (PD). Until now, molecular analyses of DA in PD have been limited to genomic or transcriptomic approaches, whereas, to the best of our knowledge, no proteomic or combined multiomic study examining the protein profile of these neurons is currently available. In this exploratory study, we used laser capture microdissection to extract regions from DA in 10 human SNpc obtained at autopsy in PD patients and control subjects. Extracted RNA and proteins were identified by RNA sequencing and nanoliquid chromatography–mass spectrometry, respectively, and the differential expression between PD and control group was assessed. Qualitative analyses confirmed that the microdissection protocol preserves the integrity of our samples and offers access to specific molecular pathways. This multiomic analysis highlighted differential expression of 52 genes and 33 proteins, including molecules of interest already known to be dysregulated in PD, such as LRP2, PNMT, CXCR4, MAOA and CBLN1 genes, or the Aldehyde dehydrogenase 1 protein. On the other hand, despite the same samples were used for both analyses, correlation between RNA and protein expression was low, as exemplified by the CST3 gene encoding for the cystatin C protein. This is the first exploratory study analyzing both gene and protein expression of laser-dissected neuronal parts from SNpc in PD. Data are available via ProteomeXchange with identifier PXD024748 and via GEO with identifier GSE 169755.
A simple method for decellularizing a cell-derived matrix for bone cell cultivation and differentiation
Authors: Weng Weidong , Zanetti Filippo , Bovard David , Braun Bianca , Ehnert Sabrina , Uynuk-Ool Tatiana , Histing Tina , Hoeng Julia , Nussler Andreas K. , Aspera-Werz Romina H. 
Published: September 2021

AbstractThe extracellular matrix regulates cell survival, proliferation, and differentiation. In vitro two-dimensional cell experiments are typically performed on a plastic plate or a substrate of a single extracellular matrix constituent such as collagen or calcium phosphate. As these approaches do not include extracellular matrix proteins or growth factors, they fail to mimic a complex cell microenvironment. The cell-derived matrix is an alternative platform for better representing the in vivo microenvironment in vitro. Standard decellularization of a cell-derived matrix is achieved by combining chemical and physical methods. In this study, we compared the decellularization efficacy of several methods: ammonium hydroxide, sodium dodecyl sulfate (SDS), or Triton X-100 with cold or heat treatment on a matrix of Saos-2 cells. We found that the protocols containing SDS were cytotoxic during recellularization. Heat treatment at 47 °C was not cytotoxic, removed cellular constituents, inactivated alkaline phosphatase activity, and maintained the levels of calcium deposition. Subsequently, we investigated the differentiation efficiency of a direct bone coculture system in the established decellularized Saos-2 matrix, an inorganic matrix of calcium phosphate, and a plastic plate as a control. We found that the decellularized Saos-2 cell matrix obtained by heat treatment at 47 °C enhanced osteoclast differentiation and matrix mineralization better than the inorganic matrix and the control. This simple and low-cost method allows us to create a Saos-2 decellularized matrix that can be used as an in vivo-like support for the growth and differentiation of bone cells.
Detection of Isopeptide Bonds in Monoclonal Antibody Aggregates
Authors: Powell Thomas , Knight Michael J. , Wood Amanda , O’Hara John , Burkitt William 
Published: September 2021

Abstract
Purpose
A major difficulty in monoclonal antibody (mAb) therapeutic development is product aggregation. In this study, intermolecular isopeptide bonds in mAb aggregates were characterized for the first time. We aim to propose a mechanism of covalent aggregation in a model antibody using stressed studies at raised temperatures to aid in the understanding of mAb aggregation pathways.

Methods
Aggregate fractions were generated using raised temperature and were purified using size-exclusion chromatography (SEC). The fractions were tryptically digested and characterized using liquid chromatography hyphenated to tandem mass-spectrometry (LC–MS/MS).

Results
An increased amount of clipping between aspartic acid and proline in a solvent accessible loop in the constant heavy 2 (CH2) domain of the mAb was observed under these conditions. Detailed peptide mapping revealed 14 isopeptide bonds between aspartic acid at that cleavage site and lysine residues on adjacent antibodies. Two additional isopeptide bonds were identified between the mAb HC N-terminal glutamic acid or a separate aspartic acid to lysine residues on adjacent antibodies.

Conclusions
Inter-protein isopeptide bonds between the side chains of acidic amino acids (aspartate and glutamate) and lysine were characterized for the first time in mAb aggregates. A chemical mechanism was presented whereby spontaneous isopeptide bond formation could be facilitated via either the aspartic acid side chain or C-terminus.
A Combined Experimental and Atomistic Investigation of PTFE Double Transfer Film Formation and Lubrication in Rolling Point Contacts
Authors: von Goeldel Stephan , Reichenbach Thomas , König Florian , Mayrhofer Leonhard , Moras Gianpietro , Jacobs Georg , Moseler Michael 
Published: September 2021

AbstractSolid lubricants such as polytetrafluoroethylene (PTFE) are used in rolling-element bearings (REBs) when conventional lubrication (i.e. by fluids or greases) cannot be applied owing to extreme operating conditions (e.g. high temperatures or vacuum). Often a double transfer film mechanism is used with a cage acting as a lubricant reservoir resupplying the REB with solid lubricant by cage wear. An increase in service life of such bearings requires a better understanding of the transfer processes in the sliding and rolling contacts. Here, we investigate the effect of PTFE resupply on friction and lubricant film formation in a steel/steel and steel/glass rolling contact by tribometry and classical molecular dynamics (MD). A ball-on-disk tribometer is enhanced by a pin-on-disk sliding contact that transfers PTFE to the disk. The experiment allows simultaneous in situ measurement of friction and film thickness by white light interferometry in the rolling point contact. Increasing the pin load results in an increased PTFE film thickness in the rolling contact accompanied by a significant decrease in friction. To elucidate the observed film transfer and friction mechanism, sliding MD simulations with a newly developed density-functional-based, non-reactive force field for PTFE-lubricated iron oxide surfaces are performed. A strong adhesion of PTFE chains to iron oxide drives transfer film formation, whilst shear-induced chain alignment within PTFE results in reduced friction. The simulations reveal an anti-correlation between PTFE film thickness and friction coefficient—in agreement with the experiments. These investigations are a first step towards methods to control PTFE transfer film formation in REBs.
Graphic Abstract
Molecular epidemiology of respiratory syncytial virus among children and adults in India 2016 to 2018
Authors: Bandla Suresh S. , Devadiga Santhosha , Bhatt Rushil , Dsa Oliver C. , Govindakarnavar Arunkumar 
Published: September 2021

AbstractRespiratory syncytial virus (RSV) is a common cause of respiratory tract infections among children less than 5 years of age and the elderly. This study intended to determine the circulating genotypes of RSV among severe acute respiratory illness (SARI) cases during the period 2016–2018 in India, among hospitalized acute febrile illness cases of age ranging from 1 to 65 years. Throat/nasopharyngeal swab samples were subjected for testing RSV and subgroups by real-time reverse transcriptase polymerase chain reaction (RT-PCR), further sequencing and phylogenetic analysis were performed for the second hypervariable region of the G gene. RSV-A and B subtypes co-circulated during the years 2016, 2017, and 2018, with RSV-A as the dominant subtype in 2016, and RSV-B as the dominant subgroup in 2017 and 2018. Phylogenetic analysis revealed that the circulating genotypes of RSV were GA2 (16/16), of RSV-A, and GB5 (23/23) of RSV-B in the South, North, and Northeast region of India during the period between 2016 and 2018. Here we report the first study comprising the distribution of RSV-A and B genotypes in the different geographic regions of India among children and adults during the year 2016 to 2018. We also report GA2.3.7 lineage of GA2 genotype for the first time in India to the best of our knowledge.
Lung cancer and interstitial lung diseases: the lack of prognostic impact of lung cancer in IPF
Authors: Carobene Loredana , Spina Donatella , Disanto Maria Giulia , Micheletto Claudio , Mazzei Maria Antonietta , Paladini Piero , Ghiribelli Claudia , Bargagli Elena , Rottoli Paola 
Published: September 2021

AbstractLung Cancer (LC) is the first cause of death worldwide. Recently increased interest in interstitial lung diseases (ILD) has highlighted an association with lung cancer, offering interesting insights into the pathogenesis of the latter. Describe the association between lung cancer and ILD and evaluate the impact of LC on survival in these populations. We collected clinical, radiological, histologic data of 53 cases of advanced pulmonary fibrosis with lung cancer: 17 with UIP pattern (usual interstitial pneumonia, UIP/IPF-LC) and 36 with non-UIP pattern (ILD-LC). Adenocarcinoma was the most frequent histological subtype of lung cancer in all three groups and in UIP/IPF-LC developed in the lung periphery and in an advanced fibrosis context. Patients with DLCO% < 38% showed survival < 10 months, irrespective of group and development of carcinoma in UIP/IPF does not necessarily affect survival, unlike in SR-ILD. Our results confirm that the oncogenic mechanism is closely linked to fibrotic and inflammatory processes and that the development of carcinoma affects survival in SR-ILD but not in IPF.
Non-alcoholic fatty liver disease frequency and associated factors at admission of acute stroke
Authors: Mori Takahisa , Yoshioka Kazuhiro , Tanno Yuhei 
Published: September 2021

Abstract
Background/purpose of the study
If non-alcoholic fatty liver disease (NAFLD) frequency is very high in stroke patients, NAFLD may be a risk factor for stroke and identifying factors of NAFLD presence may lead to stroke prevention. This retrospective study aimed to investigate whether NAFLD frequency was very high and identify factors associated with NAFLD presence at acute stroke admission.

Methods
We included stroke patients aged 40 − 79 years who (1) were admitted from 2016 to 2019, within 24 h of onset; (2) underwent abdominal ultrasonography; and (3) underwent blood examination of biomarkers. We evaluated the frequency and significant factors of NAFLD presence.

Results
Among 1672 stroke patients, 676 patients met our inclusion criteria, and 267 patients (39.5%) had NAFLD. Compared to patients without NAFLD, patients with NAFLD were young; had high anthropometric values; high blood pressure; low aspartate aminotransferase/alanine aminotransferase ratio (AST/ALT) ratio; high levels of liver enzymes, serum albumin, HbA1c, and serum lipids; low-density lipoprotein; high serum level of some fatty acids; and high fatty acid% of palmitic acid (PA) and dihomo-gamma-linolenic acid (DGLA). After excluding variables with multicollinearity, independent NAFLD-presence factors were high body mass index (BMI), low AST/ALT ratio, high serum albumin level, high PA%, and high DGLA level.

Conclusions
The frequency of NAFLD was high in our patient group. Significant NAFLD-presence factors were high BMI, low AST/ALT ratio, high serum albumin level, high PA%, and high DGLA level. A further study is warranted to determine the effects of the NAFLD-presence factors on stroke onset or prevention.
Paleogene lake deep water sedimentary facies in the northern zone of the Chezhen Sag, Bohai Bay Basin, China
Authors: Yuanpei Zhang , Xuecai Zhang , Haifang Cao , Xugang Zheng , Jinkai Wang , Jinliang Zhang 
Published: September 2021

AbstractThis study researches an important genetic type of Paleogene sublacustrine fan deposits in widely developed glutenite bodies in the northern Chezhen Sag. The Paleogene sublacustrine fans in the Bohai Bay Basin are primarily distributed in Es3 (The third member of Paleogene Shahejie Formation). There are many sedimentary types in Chezhen depression, but understanding of the reservoir is too limited to support a development strategy. Therefore, a detailed study on the core characteristics, sedimentary structure, sedimentary microfacies and reservoir properties has been completed in order to reduce uncertainty. It is confirmed that the sublacustrine fan deposits are mostly characterized by a positive cycle of upward thinning, and the base is composed of conglomerate supporting conglomerate and massive gravel sandstone, which reflect the sedimentary characteristics of traction and gravity currents. Finally, the influence of sedimentary process on properties was assessed by establishing the correlation between microfacies and reservoir physical parameters, such as porosity, permeability, detrital particle. It is revealed that the sublacustrine fan glutenite body formed in the deep depression period is interspersed with deep- to semi-deep lake source rocks. The superior source–reservoir–caprock combination is one of the most important exploration targets in the northern zone.
Risk factors in bee and Vespula venom allergy: state of the art
Authors: Stoevesandt Johanna , Trautmann Axel 
Published: September 2021

Summary
Background
Correct recognition of risk factors enables individualized management and treatment of venom allergic patients.

Methods
Systematic research and review of current literature regarding the risk of (1) severe sting-induced anaphylaxis, (2) anaphylactic adverse event during venom immunotherapy (VIT), and (3) treatment failure.

Results and discussion
(1) Mastocytosis is the most important risk factor for severe sting-induced anaphylaxis. Hereditary α‑tryptasemia was recently identified as a genetic predictor of severe reactions. Older age is clearly associated with an increased risk; the respective impact of defined cardiovascular comorbidities has yet to be determined. Recent data do not support an aggravation of venom-induced anaphylaxis by intake of β‑blockers or angiotensin-converting enzyme (ACE) inhibitors. A higher risk in men can be attributed to more intensive exposure to stinging insects. (2) Anaphylactic side effects of VIT are most common during the buildup phase, particularly in the course of (ultra-)rush protocols involving a high number of injections and high cumulative daily doses. They are significantly more frequent during honeybee compared to Vespula VIT. Data supporting a negative effect of mastocytosis on the tolerability of VIT are scarce. Older age and cardiovascular medication are not associated with a higher incidence of VIT-induced anaphylaxis. (3) Relapsing anaphylactic reactions to both field and challenge stings are significantly more common during and after honeybee compared to Vespula VIT. Reports of severe field-sting reactions in mastocytosis patients suggest an increased risk of treatment failure which may be overcome by higher maintenance doses and longer duration of VIT.
Staffing in a Level 1 Trauma Center: Quantifying Capacity for Preparedness
Authors: Woods Kaitlin , Hayanga J.W. Awori , Cannon Jeffrey , Lemons Wesley , Philips Michael , Schmidt Ashley , Boh Roosevelt , Noor Kinza , Fornaresio Lisa , Thibault Dylan , Martin PS , Wilson Alison , Hayanga Heather K. 
Published: September 2021

AbstractObjective:We sought to determine who is involved in the care of a trauma patient.Methods:We recorded hospital personnel involved in 24 adult Priority 1 trauma patient admissions for 12 h or until patient demise. Hospital personnel were delineated by professional background and role.Results:We cataloged 19 males and 5 females with a median age of 50-y-old (interquartile range [IQR], 35.5-67.5). The average number of hospital personnel involved was 79.71 (standard deviation, 17.62; standard error 3.6). A median of 51.2% (IQR, 43.4%-59.8%) of personnel were first involved within hour 1. More personnel were involved in direct versus indirect care (median 54.5 [IQR, 47.5-67.0] vs 25.0 [IQR, 22.0-30.5];P< 0.0001). Median number of health-care professionals and auxiliary staff were 74.5 (IQR, 63.5-90.5) and 6.0 (IQR, 5.0-7.0), respectively. More personnel were first involved in hospital locations external to the emergency department (median, 53.0 [IQR, 41.5-63.0] vs 27.5 [IQR, 24.0-30.0];P< 0.0001). No differences existed in total personnel by Injury Severity Score (P= 0.1266), day (P= 0.7270), or time of admission (P= 0.2098).Conclusions:A large number of hospital personnel with varying job responsibilities respond to severe trauma. These data may guide hospital staffing and disaster preparedness policies.
Shifting Standards of Judicial Review During the Coronavirus Pandemic in the United States
Authors: Mariner Wendy K. 
Published: September 2021

AbstractEmergencies are exceptions to the rule. Laws that respond to emergencies can create exceptions to rules that protect human rights. In long lasting emergencies, these exceptions can become the rule, diluting human rights and eroding the rule of law. In the United States, the COVID-19 pandemic prompted states to change rules governing commercial and personal activities to prevent the spread of the coronavirus. Many governors’ executive orders were challenged as violations of the constitutionally protected rights of those affected. Judges are deciding whether emergencies can justify more restrictions than would be permitted in normal circumstances and whether some rights deserve more protection than others, even in an emergency. This article analyzes ongoing litigation involving emergency restrictions on religious freedom and access to reproductive health services. These cases suggest that some judges are altering the standards of judicial review of the state’s emergency powers in ways that could permanently strengthen some rights and dilute others in normal circumstances.
First morphological and molecular identification of the cercaria of Stomylotrema vicarium from the endemic apple snail Pomacea americanista
Authors: Dellagnola Federico A. , Campoy-Diaz Alejandra D. , Vega Israel A. 
Published: September 2021

AbstractThe adult fluke Stomylotrema vicarium (Stomylotrematidae, Microphalloidea) was described for the first time in Theristicus caerulescens in 1901, but the complete life cycle has remained unknown to date. Here, we found a stomylotrematid trematode in the digestive gland of the endemic apple snail Pomacea americanista. The digestive gland's tubuloacini were compressed by the trematode larvae placed on connective tissues and haemocoel spaces. Non-virgulate, stylet-bearing cercariae showed three pairs of penetration glands with a body, oral sucker and stylet morphometrically similar to those of stylet-bearing, unencysted young metacercariae of S. vicarium found in the aquatic coleopteran Megadytes glaucus, and at a lesser extent with cercariae of S. gratiosus found in the apple snail Pomacea maculata. The larvae molecular phylogeny was inferred using the markers rRNA 28S and ITS1, being these sequences grouped with the sequences of S. vicarium obtained from adult flukes. Together, these findings indicate that the life cycle of S. vicarium begins in P. americanista, thus supporting the hypothesis that the ampullariid snails act as a first intermediate host.
A qualitative investigation of the psychosocial services utilised by care-givers of patients with Alzheimer's disease and related dementias in southwestern Uganda
Authors: Abaasa Catherine , Obua Celestino , Wakida Edith K. , Rukundo Godfrey Zari 
Published: September 2021

AbstractIndividuals with Alzheimer's disease and related dementias often require substantial support from other people. Much of the care-giving is from family members who eventually experience physical, emotional and financial stress, depression and fatigue. In Uganda, families are a cornerstone in providing care to individuals with dementia. However, little is known about the psychosocial supports available to the care-givers in their care-giving role. We assessed the psychosocial supports available to care-givers of individuals with Alzheimer's disease and related dementias in southwestern Uganda. We conducted 34 in-depth interviews at three referral hospitals at which care-givers identified by the treating clinicians were approached for informed consent. The interviews were conducted until thematic saturation was reached, and the interviews were translated and transcribed. Thematic content analysis was used to analyse the data. Care-giver supports were structured into two major themes: medical supports utilised and supports beyond the medical care system. Medical supports highlighted information provided by medical professionals. Supports beyond the medical care system included emotional and instrumental supports provided by religious leaders, the local communities and family members. Care-givers for individuals with dementia in southwestern Uganda receive educational support from medical practitioners, and unstructured emotional and instrumental supports from the family and community.