bluuebunny/crossref_metadata_2025_split huggingface dataset:
Adolescent Binge Drinking Is Associated With Accelerated Decline of Gray Matter Volume
Authors: Infante M A , Eberson S C , Zhang Y , Brumback T , Brown S A , Colrain I M , Baker F C , Clark D B , De Bellis M D , Goldston D , Nagel B J , Nooner K B , Zhao Q , Pohl K M , Sullivan E V , Pfefferbaum A , Tapert S F , Thompson W K 
Published: September 2021

Abstract
The age- and time-dependent effects of binge drinking on adolescent brain development have not been well characterized even though binge drinking is a health crisis among adolescents. The impact of binge drinking on gray matter volume (GMV) development was examined using 5 waves of longitudinal data from the National Consortium on Alcohol and NeuroDevelopment in Adolescence study. Binge drinkers (n = 166) were compared with non-binge drinkers (n = 82 after matching on potential confounders). Number of binge drinking episodes in the past year was linked to decreased GMVs in bilateral Desikan–Killiany cortical parcellations (26 of 34 with P &lt; 0.05/34) with the strongest effects observed in frontal regions. Interactions of binge drinking episodes and baseline age demonstrated stronger effects in younger participants. Statistical models sensitive to number of binge episodes and their temporal proximity to brain volumes provided the best fits. Consistent with prior research, results of this study highlight the negative effects of binge drinking on the developing brain. Our results present novel findings that cortical GMV decreases were greater in closer proximity to binge drinking episodes in a dose–response manner. This relation suggests a causal effect and raises the possibility that normal growth trajectories may be reinstated with alcohol abstinence.
UHPLC–MS/MS Method for Quantifying Fangchinoline, Tetrandrine and Calycosin-7-O-β-D-Glucoside of Fangji Huangqi Decoction in Rat Plasma and Its Application to a Pharmacokinetic Study
Authors: Guan Jiao , Wang Liming , Ji Yuan , Zhang Tianyi , Sang Ye , Chang Sheng , Feng Bo , Zhu Heyun 
Published: September 2021

Abstract
Fangji Huangqi Decoction is composed of Stephaniae Tetrandrae Radix, Astragli Radix, Atractylodis Macrocephalae Rhizoma and Glycyrrhizae Radix Et Rhizoma. It is a classic traditional Chinese medicine formula for the treatment of chronic glomerulonephritis in China. However, its pharmacokinetic characteristics in vivo are still unclear. In this study, a method for quantifying fangchinoline, tetrandrine and calycosin-7-O-β-D-glucoside, the main active constituents of Fangji Huangqi Decoction, in rat plasma by using ultrahigh-performance liquid chromatography–tandem mass spectrometry technique was developed. Plasma samples were processed with a deproteinization procedure using acetonitrile, followed by chromatographic separation on a Shim-pack XR-ODS C18 column using gradient elution of 0.1% aqueous formic acid and acetonitrile at 0.4 mL/min. The analytes and internal standard, diphenhydramine hydrochloride, were detected using positive electrospray ionization in multiple reactions monitoring mode. The optimized mass transition ion-pairs (m/z) were 609.3/367.3 for fangchinoline, 623.3/174.3 for tetrandrine, 447.2/285.1 for calycosin-7-O-β-D-glucoside and 256.2/167.1 for diphenhydramine hydrochloride, respectively. The developed method was validated for intraday and interday precision and accuracy whose values fell in the acceptable limits. Recovery efficiency of all the analytes was found to be &gt;90.5%. Matrix effect was found to be negligible. Stability results showed that the analytes were stable under all conditions. The validated method was successfully used for studying the pharmacokinetics of the three compounds in rat plasma after oral administration of Fangji Huangqi Decoction.
Purification of Recombinant Mouse C-Reactive Protein fromPichia PastorisGS115 by Nickel Chelating Sepharose Fast-Flow Affinity Chromatography andP-Aminophenyl Phosphoryl Choline Agarose Resin Affinity Chromatography in Tandem
Authors: Cheng Bin , Wu Di , Wu Ke , Huang Xiao-Ping , Lv Jian-Min , Ji Shang-Rong , Zhu Li 
Published: September 2021

AbstractC-reactive protein (CRP) is a circulating marker of inflammation yet with ill-defined biological functions. This is partly due to the uncharacterized activities of endogenous CRP in mice, the major animal model used to define protein function. The hurdles for purification and characterization of mouse CRP are its low circulating levels and the lack of specific antibodies. To clear these hurdles, here we developed an efficient expression system by constructing recombinant Pichia pastoris cells for secretion of native conformation mouse CRP. The recombinant expression of mouse CRP in Escherichia coli failed to yield sufficient amount of native protein, reflecting the importance of post-translational modification of glycosylation in aiding proper folding. By contrast, sufficient amount of native mouse CRP was successfully purified from P. pastoris. Preliminary purification was performed by Nickel Chelating Sepharose Fast-Flow affinity chromatography with 6 × His tags attached to the protein. Subsequently, p-Aminophenyl Phosphoryl Choline Agarose resin affinity chromatography was used for tandem purification. The purified mouse CRP showed native pentamer and capabilities of PC binding. Moreover, the 6 × His tag provides a convenient tool for detecting the interactions of mouse CRP with ligands.
Effectiveness of Four Different Interventions Against Schistosoma haematobium in a Seasonal Transmission Setting of Côte d’Ivoire: A Cluster Randomized Trial
Authors: Ouattara Mamadou , Bassa Fidèle K , Diakité Nana R , Hattendorf Jan , Coulibaly Jean T , Yao Patrick K , Tian-Bi Yves-Nathan T , Konan Cyrille K , Assaré Rufin K , Koné Naférima , Guindo-Coulibaly Négnorogo , Utzinger Jürg , N’Goran Eliézer K 
Published: September 2021

Abstract

Background
Annual mass drug administration (MDA) using praziquantel is the cornerstone of schistosomiasis morbidity control but is not sufficient to interrupt transmission. We implemented a cluster-randomized trial to compare the effectiveness of 4 different intervention packages to interrupt transmission of Schistosoma haematobium in a seasonal transmission setting of Côte d’Ivoire.

Methods
Sixty-four localities with a S. haematobium prevalence in school children aged 13–14 years above 4% were randomly assigned to 1 of 4 intervention arms over a 3-year period: (1) the current standard strategy consisting of annual MDA before peak of transmission, (2) annual MDA after peak of transmission, (3) biannual MDA, and (4) standard MDA combined with snail control. The primary outcome was prevalence and intensity of S. haematobium infection in children aged 9–12 years 1 year after the final intervention, using urine filtration performed by experienced microscopists.

Results
By study end, we observed the lowest S. haematobium prevalence in the biannual MDA, compared to the standard treatment arm (0.6% vs 7.5%; odds ratio [OR] = 0.07, 95% confidence interval [CI] = .02 to .24). The prevalence in arms 2 and 4 was about 3.5%, which was not statistically significantly different from the standard strategy (both ORs 0.4, 95% CI = .1 to ~1.8). New cases of infection were still observed in all arms at study end.

Conclusions
Biannual MDA was the only regimen that outperformed the standard treatment. All strategies resulted in decreased prevalence of infection; however, none of them was able to interrupt transmission of S. haematobium within a 3-year period.

Clinical Trials Registration
ISRCTN10926858.
South Indian Children’s Neurodevelopmental Outcomes After Group B Streptococcus Invasive Disease: A Matched-Cohort Study
Authors: John Hima B , Arumugam Asha , Priya Mohana , Murugesan Nandhini , Rajendraprasad Nandhini , Rebekah Grace , Paul Proma , Chandna Jaya , Lawn Joy E , Santhanam Sridhar 
Published: September 2021

Abstract

Background
This study is part of a multicountry matched-cohort study designed to estimate the risk of long-term neurodevelopmental impairment (NDI) of children exposed to invasive group B Streptococcus (iGBS). The specific objective of this paper is to compare NDI across domains of iGBS survivors with a matched non iGBS group in our population.

Methods
Survivors of iGBS in a South Indian hospital were identified and recruited between January 2020 and April 2021. Cases were compared with age- and gender-matched non iGBS children. Participants were assessed using Bayley Scales of Infant and Toddler Development–3rd edition (BSID-III), Wechsler Preschool and Primary Scale of Intelligence–4th edition (WPPSI-IV), Wechsler Intelligence Scale for Children–5th edition (WISC-V), Child Behavior Checklist (CBCL), and Bruininks-Oseretsky Test of Motor Proficiency, 2nd edition (BOT-2), depending on age.

Results
Our cohort comprised 35 GBS-exposed and 65 matched non iGBS children, aged 1–14 years. The iGBS-exposed group had 17 (48.6%) children with impairment in ≥1 domain compared to 25 (38%) in the non iGBS group (unadjusted OR, 1.51; 95% CI, .65–3.46), 9 (26%) children with “multi-domain impairment” compared to 10 (15.4%) in the non iGBS group (unadjusted OR, 1.90; 95% CI, .69–5.24), and 1 (2.9%) child with moderate to severe impairment compared to 3 (4.6%) in the non iGBS group (unadjusted OR, .60; 95% CI, .06–6.07). In the iGBS group, more children had motor impairments compared with the non iGBS group (unadjusted OR, 10.7; 95% CI, 1.19–95.69; P = .034).

Conclusions
Children with iGBS seem at higher risk of developing motor impairments compared with a non iGBS group.
Stakeholder Perceptions About Group B Streptococcus Disease and Potential for Maternal Vaccination in Low- and Middle-Income Countries
Authors: Mantel Carsten , Cherian Thomas , Ko Melissa , Malvolti Stefano , Mason Elizabeth , Giles Michelle , Lambach Philipp 
Published: September 2021

Abstract

Background
To inform the World Health Organization’s full value of vaccine assessment for group B Streptococcus (GBS) vaccines, a rapid literature appraisal was conducted to inform the operationalization of maternal GBS vaccination. We found limited published information on stakeholder perceptions of the public health importance of GBS disease and vaccination, and we therefore undertook a multicountry survey.

Methods
An online survey was conducted in late 2019 to collect information on stakeholders’ awareness of GBS disease and the priority accorded to vaccination. The survey was distributed by email to 395 representatives of national pediatric, gynecology, and obstetrics associations, national immunization technical advisory groups (NITAGs), national regulatory agencies, academia, and United Nations organizations.

Results
Among 101 survey respondents from 66 countries, 36% were pediatricians, 25% obstetricians/gynecologists, 21% immunization specialists, and 18% other public health specialists. More than half (58%) of respondents reported being familiar with GBS disease as a public health problem; familiarity decreased by country income level. Knowledge of GBS disease was greatest in the Americas (68%) and Europe (66%) and lowest in Asia (13%–38%). Perception of GBS disease as a public health problem was highest among pediatricians (71%) and lowest among public health policy makers and NITAG members (30%) across country groupings. Approximately half of respondents (49%) considered the introduction of a GBS vaccine as a priority.

Conclusions
The information obtained will inform the appropriate packaging and presentation of information to address stakeholder perceptions and promote evidence-based decision making on GBS vaccination.
Bee and Flowering Plant Communities in a Riparian Corridor of the Lower Rio Grande River (Texas, USA)
Authors: Rubio Amede , Wright Karen , Longing Scott 
Published: September 2021

AbstractThe Rio Grande in Texas is the geopolitical boundary between the United States and Mexico. Considered one of the world’s most at-risk rivers, it has been impacted by intensified management by both countries sharing its watershed. Invasion by Arundo donax (Linnaeus) (Poales: Poaceae), giant reed, has been extensive in the riparian corridor, with potential impacts on native wildlife. A need exists to better understand the ecological communities in these habitats to support strategies for enhancing resources for pollinators. We sampled bee and flowering plant communities monthly over 2 yr along a 3.22 km stretch of the lower Rio Grande in Webb County, TX. Bee and plant richness and abundance were bimodal with peaks in March–April and September in both riparian and upland habitats. The bee community was similar across habitats and sampling dates and dominated by a few common species. Anthophora occidentalis (Cresson) (Hymenoptera: Apidae) and Lasioglossum sp. L (Curtis) (Hymenoptera: Apidae) were indicator species of the riparian habitat, and Halictus ligatus (Say) (Hymenoptera: Halictidae) was an indicator species of the upland habitat. Three plant species were indicator species in riparian habitats, spiny pricklepoppy (Argemone sanguinea Greene) (Papaverales: Papaveraceae), spotted beebalm (Monarda punctata Linnaeus) (Lamiales: Lamiaceae), and Pennsylvania cudweed (Gamochaeta pensylvanica Willdenow) (Asterales: Asteraceae). Analysis showed a positive relationship between bee richness and abundance with flowering plant diversity, increasing bee richness within an optimal temperature range 25−30°C, and higher bee abundance with increased average monthly precipitation. This geographically extensive riparian corridor could be managed using ecological restoration to enhance resources for pollinators.
Computed tomography to identify risk factors for left circumflex artery injury during mitral surgery
Authors: Kishimoto Noriaki , Takahashi Yosuke , Fujii Hiromichi , Sakon Yoshito , Izuta Shinichiro , Kitada Ryoko , Morisaki Akimasa , Yoshida Hisako , Ehara Shoichi , Shibata Toshihiko 
Published: September 2021

Abstract

OBJECTIVES
Cases in which the left circumflex coronary artery (LCX) runs close to the mitral annulus are considered high risk for LCX injury during mitral surgery. We investigated the anatomical relationship between the LCX and the mitral annulus using 3-dimensional (3D) computed tomography (CT).

METHODS
We constructed 3D-CT images of the LCX and the mitral annulus before surgery in 122 patients with mitral regurgitation (MR). We classified coronary dominance by 3D-CT and MR aetiologies (degenerative, atrial functional MR, ventricular functional MR and Barlow’s disease) using echocardiography. We detected the point on the mitral annulus closest to the LCX (X point) and measured the minimum distance from the LCX to the mitral annulus (mCAD). We judged whether atrioventricular disjunction existed using CT. We also investigated the factors affecting mCAD and examined how coronary dominance and MR aetiologies relate to the location of the X point.

RESULTS
The median mCAD was 4.2 mm (range 0.9–11.4 mm). Considering coronary dominance and MR aetiologies, mCAD was shorter in patients with left coronary dominance and Barlow’s disease. The X point mostly existed on the lateral side of the posterior annulus, but it sometimes existed on the medial side. Multiple regression revealed left dominance and mitral annular disjunction as significant factors affecting mCAD (P = 0.01).

CONCLUSIONS
The anatomical relationship between the LCX and the mitral annulus can be recognized using superimposed 3D-CT images. This approach is useful to avoid LCX injury in mitral valve surgery since the X point varies between patients.
European Society of Cardiology guidance for the diagnosis and management of cardiovascular disease during the COVID-19 pandemic: part 1—epidemiology, pathophysiology, and diagnosis
Authors:   The Task Force for the management of COVID-19 of the European Society of Cardiology, Baigent Colin , Windecker Stephan , Andreini Daniele , Arbelo Elena , Barbato Emanuele , Bartorelli Antonio L , Baumbach Andreas , Behr Elijah R , Berti Sergio , Bueno Héctor , Capodanno Davide , Cappato Riccardo , Chieffo Alaide , Collet Jean-Philippe , Cuisset Thomas , de Simone Giovanni , Delgado Victoria , Dendale Paul , Dudek Dariusz , Edvardsen Thor , Elvan Arif , González-Juanatey José R , Gori Mauro , Grobbee Diederick , Guzik Tomasz J , Halvorsen Sigrun , Haude Michael , Heidbuchel Hein , Hindricks Gerhard , Ibanez Borja , Karam Nicole , Katus Hugo , Klok Fredrikus A , Konstantinides Stavros V , Landmesser Ulf , Leclercq Christophe , Leonardi Sergio , Lettino Maddalena , Marenzi Giancarlo , Mauri Josepa , Metra Marco , Morici Nuccia , Mueller Christian , Petronio Anna Sonia , Polovina Marija M , Potpara Tatjana , Praz Fabien , Prendergast Bernard , Prescott Eva , Price Susanna , Pruszczyk Piotr , Rodríguez-Leor Oriol , Roffi Marco , Romaguera Rafael , Rosenkranz Stephan , Sarkozy Andrea , Scherrenberg Martijn , Seferovic Petar , Senni Michele , Spera Francesco R , Stefanini Giulio , Thiele Holger , Tomasoni Daniela , Torracca Luccia , Touyz Rhian M , Wilde Arthur A , Williams Bryan 
Published: September 2021

AbstractAimsSince its emergence in early 2020, the novel severe acute respiratory syndrome coronavirus 2 causing coronavirus disease 2019 (COVID-19) has reached pandemic levels, and there have been repeated outbreaks across the globe. The aim of this two-part series is to provide practical knowledge and guidance to aid clinicians in the diagnosis and management of cardiovascular disease (CVD) in association with COVID-19.Methods and resultsA narrative literature review of the available evidence has been performed, and the resulting information has been organized into two parts. The first, reported here, focuses on the epidemiology, pathophysiology, and diagnosis of cardiovascular (CV) conditions that may be manifest in patients with COVID-19. The second part, which will follow in a later edition of the journal, addresses the topics of care pathways, treatment, and follow-up of CV conditions in patients with COVID-19.ConclusionThis comprehensive review is not a formal guideline but rather a document that provides a summary of current knowledge and guidance to practicing clinicians managing patients with CVD and COVID-19. The recommendations are mainly the result of observations and personal experience from healthcare providers. Therefore, the information provided here may be subject to change with increasing knowledge, evidence from prospective studies, and changes in the pandemic. Likewise, the guidance provided in the document should not interfere with recommendations provided by local and national healthcare authorities.
Mapping causal genes and genetic interactions for agronomic traits using a large F2 population in rice
Authors: Feng Laibao , Ma Aimin , Song Bo , Yu Sibin , Qi Xiaoquan 
Published: September 2021

AbstractDissecting the genetic mechanisms underlying agronomic traits is of great importance for crop breeding. Agronomic traits are usually controlled by multiple quantitative trait loci (QTLs) and genetic interactions, and mapping the underlying causal genes is still labor-intensive and time-consuming. Here, we present a genetic tool for directly targeting the specific causal genes by using a single-gene resolution linkage map that was constructed from 3756 F2 rice plants via targeted sequencing technology and Tukey-Kramer multiple comparisons test. Three large- and moderate-effect QTLs, qHD6-2, qGL3-1, and qGW5-2, were successfully mapped to their specific causal genes, Hd1, GS3, and GW5, respectively. A complex genetic interaction network containing 30 QTL–QTL interactions was constructed, revealing that the alternative allele of hub QTL, qHD6-2, can hide or release the genetic contributions of the alleles at interacting loci. Moreover, arranging genetic interactions in the models lead to more accurate phenotypic predictions. These results provide a community resource and new feasible strategy for deciphering the genetic mechanisms of complex agronomic traits and accelerating crop breeding.
Aegilops tauschii genome assembly Aet v5.0 features greater sequence contiguity and improved annotation
Authors: Wang Le , Zhu Tingting , Rodriguez Juan C , Deal Karin R , Dubcovsky Jorge , McGuire Patrick E , Lux Thomas , Spannagl Manuel , Mayer Klaus F X , Baldrich Patricia , Meyers Blake C , Huo Naxin , Gu Yong Q , Zhou Hongye , Devos Katrien M , Bennetzen Jeffrey L , Unver Turgay , Budak Hikmet , Gulick Patrick J , Galiba Gabor , Kalapos Balázs , Nelson David R , Li Pingchuan , You Frank M , Luo Ming-Cheng , Dvorak Jan 
Published: September 2021

Abstract
Aegilops tauschii is the donor of the D subgenome of hexaploid wheat and an important genetic resource. The reference-quality genome sequence Aet v4.0 for Ae. tauschii acc. AL8/78 was therefore an important milestone for wheat biology and breeding. Further advances in sequencing acc. AL8/78 and release of the Aet v5.0 sequence assembly are reported here. Two new optical maps were constructed and used in the revision of pseudomolecules. Gaps were closed with Pacific Biosciences long-read contigs, decreasing the gap number by 38,899. Transposable elements and protein-coding genes were reannotated. The number of annotated high-confidence genes was reduced from 39,635 in Aet v4.0 to 32,885 in Aet v5.0. A total of 2245 biologically important genes, including those affecting plant phenology, grain quality, and tolerance of abiotic stresses in wheat, was manually annotated and disease-resistance genes were annotated by a dedicated pipeline. Disease-resistance genes encoding nucleotide-binding site domains, receptor-like protein kinases, and receptor-like proteins were preferentially located in distal chromosome regions, whereas those encoding transmembrane coiled-coil proteins were dispersed more evenly along the chromosomes. Discovery, annotation, and expression analyses of microRNA (miRNA) precursors, mature miRNAs, and phasiRNAs are reported, including miRNA target genes. Other small RNAs, such as hc-siRNAs and tRFs, were characterized. These advances enhance the utility of the Ae. tauschii genome sequence for wheat genetics, biotechnology, and breeding.
Replication timing analysis in polyploid cells reveals Rif1 uses multiple mechanisms to promote underreplication in Drosophila
Authors: Das Souradip , Caballero Madison , Kolesnikova Tatyana , Zhimulev Igor , Koren Amnon , Nordman Jared 
Published: September 2021

Abstract
Regulation of DNA replication and copy number is necessary to promote genome stability and maintain cell and tissue function. DNA replication is regulated temporally in a process known as replication timing (RT). Rap1-interacting factor 1 (Rif1) is a key regulator of RT and has a critical function in copy number control in polyploid cells. Previously, we demonstrated that Rif1 functions with SUUR to inhibit replication fork progression and promote underreplication (UR) of specific genomic regions. How Rif1-dependent control of RT factors into its ability to promote UR is unknown. By applying a computational approach to measure RT in Drosophila polyploid cells, we show that SUUR and Rif1 have differential roles in controlling UR and RT. Our findings reveal that Rif1 acts to promote late replication, which is necessary for SUUR-dependent underreplication. Our work provides new insight into the process of UR and its links to RT.