bluuebunny/crossref_metadata_2025_split huggingface dataset:
Adherence to Inhalers and Associated Factors Among Adult Asthma Patients: an Outpatient Based Study in a Tertiary Hospital of Rajshahi, Bangladesh
Authors: Rafi Md. Abdur , Tahmin Chowdhury Ibtida , Tashrik Symom , Bonna Atia Sharmin , Jannat Ferdousy , Mily Sabrina Jahan , Shrestha Abhigan babu , Seemanta Senjuti , Rashid Afsana , Mahjabeen Mosarrat , Nura Nurunnahar , Shahriar Tasnim , Mahadi Ashrafur Rahaman , Ahmed Kawser , Hasan Md. Jahid , Haque Md. Azizul , Hossain Md. Golam 
Published: December 2021

Abstract
Background: Adherence to inhaler medication is an important contributor of optimum asthma control along with adequate pharmacotherapy. The objective of the present study was to assess self-reported adherence level and to identify the potential factors associated with non-adherence to the inhalers among asthma patients.Methods: This facility-based cross-sectional study was conducted in the medicine outpatient department of Rajshahi Medical College Hospital from November 2020 to January 2021. A total of 357 clinically confirmed adult asthma patients were interviewed. Inhaler adherence was measured using the 10-item Test of Adherence scale (TAI). . Both descriptive and inferential statistics was used to express the socio-demographic of the patients and predictors of poor adherence to inhaler. Results: A substantial number of participants were non-adherent (86%) to inhaler medication. Patient non-adherent to inhaler medication are often younger (23.15, 95% CI 3.67-146.08), lived in rural area ( 23.28, 95% CI 2.43-222.66), less year of schooling (5.69, 95% CI 1.27-25.44), and belonged to the middle income (aOR 9.74, 95% CI 2.11-44.9) than those adherent with inhaler. Presence of comorbidities (12.91, 95% CI 1.41-117.61), prolonged duration of inhaler intake (5.69, 95% CI 1.22-26.49), consulting non-qualified practitioners (13.09, 95% CI 3.10-55.26) were the significant contributor of non-adherence.Conclusion: Despite ongoing motivation and treatment, non-adherence to inhalation anti-asthmatic is high and several factors have been found contributed. Regular monitoring and a guided patient centered self-management approach might be helpful to address the in long run.
Production of Recombinant Human EGFR CR2 Domain and Generation of Monoclonal Antibodies Discriminating The R and K Variants.
Authors: Marzouq Maryam , Alshammary Amal , Bourguiba-Hachemi Sonia , Raslan Waseem , Ashoor Dana , Fathallah M. Dahmani 
Published: December 2021

Abstract
Background: The epidermal growth factor receptor (EGFR) was the first molecular marker to be targeted successfully in monoclonal antibodies-based cancer immunotherapy. The human EGFR displays two common natural genetic variants (R521K) located in the molecule extracellular CR2 domain. This polymorphism affects the outcome of cancer immunotherapy using anti-EGFR mAbs.Results: In this paper, we report the production, purification and characterization of recombinant forms of the EGFR/CR2-R and CR2-K variants as Glutathione S-transferase (GST) fusion protein in E. Coli BL21. We used these two proteins to generate three different murine monoclonal antibodies to the EGFR CR2 domain (anti-CR2R, anti-CR2K and anti-CR2-RK). We carried out the molecular characterization of the anti-CR2-RK mAb. Analysis using Western blot, ELISA and Immunohistochemistry of various tumor tissues samples, showed that anti-CR2RK mAb was specific of the human EGFR CR2 extracellular domain and recognizes equally both the CR2-K and CR2-R natural genetic variants. In addition, the affinity binding of anti-CR2-RK mAb, as determined by Surface Plasmon Resonance, was equal to 27.7KD μM. Conclusions: We produced recombinant forms of the human EGFR CR2 domain R and K variants and generated mAbs that discriminate between them. These mAbs can be engineered into novel cancer therapeutic and or diagnostic tools tailored to the patients EGFR genetic makeup.
Microporous Polymer Adsorptive Membranes with Unprecedented Processing Capacity for Molecular Separation
Authors: Wang Zhenggong , Luo Xiaofan , Lu Kuan , Zhu Shouwen , Yang Yanshao , Song Zejun , Fang Wangxi , Zhang Yatao , Jin Jian 
Published: December 2021

Abstract
Trade-off between permeability and nanometer-level selectivity is an inherent shortcoming of membrane-based separation of molecules, while most highly porous materials with high adsorption capacity lack solution processability and stability for achieving adsorption-based molecule separation. We hereby report a hydrophilic amidoxime modified polymer of intrinsic microporosity (AOPIM-1) as a membrane adsorption material to selectively adsorb and separate small organic molecules from water with ultrahigh processing capacity. The membrane adsorption capacity for Rhodamine B reaches 26.114 g m−2, 10~1000 times higher than previously reported adsorptive membranes. Meanwhile, the membrane achieves >99.9% removal of various nano-sized organic molecules with water flux 2 orders of magnitude higher than typical pressure-driven membranes of similar rejections. This work confirms the feasibility of microporous polymers for membrane adsorption with unprecedented capacity, and provides the possibility of adsorptive membranes for molecular separation.
Hydrocoel Morphogenesis Forming the Pentaradial Body Plan in a Sea Cucumber, Apostichopus Japonicus
Authors: Udagawa Sumio , Ikeda Takafumi , Oguchi Kohei , Kohtsuka Hisanori , Miura Toru 
Published: December 2021

Abstract
Echinoderms constitute an animal phylum characterized by the pentaradial body plan. During the development from bilateral larvae to pentaradial adults, the formation of the multiple of five hydrocoel lobes, i.e., the buddings from the mesodermal coelom, is the firstly emerging pentameral character. The developmental mechanism underlying the hydrocoel-lobe formation should be revealed to understand the evolutionary process of this unique and highly derived body plan of echinoderms, although the morphogenetic mechanisms of hydrocoel lobes is largely uninvestigated. In this study, using the sea cucumber Apostichopus japonicus, in which the hydrocoel is easily observable, the developmental process of hydrocoel lobes was described in detail, focusing on the cell proliferation and rearrangement. Cell proliferation was not specifically distributed in the growing tips of the hydrocoel lobes and inhibition of the cell proliferation did not affect the lobe formation. During lobe formation, epithelium of the hydrocoel lobes were firstly stratified and then transformed into single-layered, suggesting that radial cell intercalation contributes to hydrocoel-lobe formation.
GBP4 is an Accurate Diagnostic Biomarker and a Potential Treatment Target for Crohn’s Disease
Authors: Shi Heng , Peng Qin , Zhou Xian-Ling , Zhu Shi-Ping , Sun Sheng-Yun 
Published: December 2021

Abstract
Background: Extensive evidence has shown that immune cell infiltration is associated with the pathogenesis of Crohn’s disease (CD). In the present study, we explored the potential mechanism underlying the pathogenesis biomarkers for CD.Methods: The GSE179285 dataset containing sequence data for intestinal mucosal was downloaded from the Gene Expression Omnibus (GEO) database. Differentially expressed genes (DEGs) in the intestinal mucosa of CD patients and healthy individuals were then identified. The infiltration pattern of 22 immune cell types was assessed using the CIBERSORT algorithm. The DEGs and 22 immune cell types were combined to find the key gene network using weighted gene co-expression network analysis (WGCNA), and pathway enrichment analyzes were performed on the hub module in the WGCNA. A linear regression model for the relationship between the expression of the hub genes in CD patients and infiltration of immune cells were also developed. The utility and accuracy of the hub genes for CD diagnosis were assessed using receiver operating characteristic (ROC) analysis. The accuracy of the model was validated using GSE20881 dataset. Results: There were 1135 DEGs between the intestinal mucosal tissue of CD patients and healthy individuals. Of these DEGs, 711 genes were upregulated, whereas 424 of them were downregulated. There was also a significant difference in the infiltration of immune cells to the intestinal mucosal between the CD patients and healthy individuals. WGCNA revealed that the turquoise module genes were strongly correlated with the infiltration of M1 macrophages (cor=0.68, p=10-16). Pathway enrichment analysis further showed the genes in the turquoise module mainly regulated the secretion of interferon-gamma and other immune effector molecules. Finally, the expression of GBP4, the identified hub gene, strongly correlated with the infiltration of M1 macrophages (adjusted r-squared=0.661, p<2x10-16), and is a relatively good marker for CD diagnostic prediction (AUC=0.736). The relationship between GBP4 expression and infiltration of M1 macrophages (adjusted r-squared=0.435, p<2x10-16) and prognostic value of the gene (AUC=0.702) were verified using the GSE20881 validation dataset.Conclusion: GBP4 is a potential biomarker for accurate CD diagnosis. The expression of GBP4 promotes the infiltration of M1 macrophages to the intestinal mucosa of CD patients.
Inhibitory Effects of LOXL2 Knockdown On Cellular Functions of Liver Cancer Stem Cells
Authors: Li Na , Gu Huan , Liu Liu , Zhang Xiao Li , Cheng Qiu Luo , Zhu Ying 
Published: December 2021

Abstract
Background and aim: Lysyl oxidase-like 2 (LOXL2) plays a role in tumor microenvironment formation and metastasis of hepatocellular carcinoma (HCC), which has a high mortality burden. Liver cancer stem cells (LCSCs) are related with the major malignant phenotypes of HCC. The function of LOXL2 in regulation of LCSCs remains unknown.Methods: CD133+HepG2 and CD133+Hep3B cells were sorted by fluorescence-activated cell sorting (FACS) from two human hepatoblastoma cell lines. Spheroid formation, apoptosis, cell cycle, as well as transwell assays were performed upon LOXL2 knock down in CD133+HepG2 and CD133+Hep3B cells. Protein and mRNA levels were quantified by Western blotting, Immunofluorescence and real-time PCR. Results: Knockdown of LOXL2 decreased spheroid formation, migration and invasion (p < 0.05), also induced apoptosis (p < 0.05) and cell cycle arrest (p < 0.05) in CD133+HepG2 and CD133+Hep3B cells. Knockdown of LOXL2 effectively inhibited expression of the anti-apoptosis proteins baculoviral IAP repeat-containing 3 (BIRC3) and murine double minute 2 (MDM2) (p < 0.01), as well as autophagy marker microtubule-associated protein 1 light chain 3 B (LC3) and autophagy gene ATG5 in CD133+HepG2 and CD133+Hep3B cells (p < 0.01). Conclusions: The results revealed that LOXL2 inhibition could reduce the proliferation and expansion of LCSCs, making LOXL2 inhibitors an attractive and novel therapeutic strategy of HCC.
Research On Arc Fault Detection Using Morlet Wavelet Features And ResNet
Authors: Zhang Shuai , Qu Na , Zheng Tianfang , Hu Congqiang 
Published: December 2021

Abstract
Series arc fault is the main cause of electrical fire in low-voltage distribution system. A fast and accurate detection system can reduce the risk of fire effectively. In this paper, series arc experiment is carried out for different kinds of electrical load. The time-domain current is analyzed by Morlet wavelet. Then, the multiscale wavelet coefficients are expressed as the coefficient matrix. We use HSV color index to map the coefficient matrix to the phase space image. Random gamma transform and random rotation are applied to data enhancement. Finally, typical deep residual network (ResNet) is established for image recognition. Training results show that this method can detect faults in real time. The accuracy of ResNet50 is 96.53% by using the data set in this paper.
Risk Factors for Secondary Glaucoma in Patients with Vogt-Koyanagi-Harada Disease
Authors: Alvarez-Guzman Carlos , Hartleben-Matkin Curt , Ruiz-Lozano Raul E. , Rodríguez-Garcia Alejandro , Quiroga-Garza Manuel E. , Valdez-Garcia Jorge E. 
Published: December 2021

Abstract
Background: To identify the prevalence and risk factors for secondary glaucoma among Mexican-mestizo patients with Vogt-Koyanagi-Harada Disease (VKH). A retrospective cohort study was conducted to identify the risk factors for developing secondary glaucoma based on demographic, clinical, and epidemiological variables of VKH Mexican-mestizo patients. Risk estimates were calculated using a Cox proportional hazards regression model. Main text: One hundred eyes of 50 patients, 44 (88%) women and 6 men (12%) with a median age of 35.5 years (IQR 29 – 46), and a median follow-up time of 72 months (IQR 13.7 – 126.7) were included for analysis. The prevalence of glaucoma was 20%, with angle-closure glaucoma accounting for 70% of all cases. Significant clinical risk factors for glaucoma development were a chronic recurrent stage at presentation (RR 2.88 95% CI 1.11 – 12.63, p=0.037), more than two episodes of recurrent anterior uveitis (RR 8.52 95% CI 2.02 – 35.92, p<0.001), angle-closure disease (ACD, RR 7.08 95% CI 2.44 – 20.48, p<0.001), iris bombé (RR 5.0 95% CI 2.10 – 11.90, p<0.001), and peripapillary atrophy (RR 3.56 95% CI 1.43 – 8.85, p<0.001). Exposure to prednisone for more than 24 months (RR 9.33 95% CI 2.21 – 39.28, p<0.001) or topical corticosteroid drops for more than 12 months (RR 3.88 95% CI 1.31 – 11.46, p=0.007) were associated with an increased likelihood for secondary glaucoma development. Conclusions: Glaucoma is a frequent complication in patients with VKH, often attributed to mixed pathogenic mechanisms. Chronic disease at presentation, recurrent inflammation, angle-closure mechanisms, iris bombé, and peripapillary atrophy represent clinically significant risk factors for secondary glaucoma development. Prompt and aggressive steroid-spearing immunosuppressive therapy for reaching adequate control of inflammation may lower the risk of glaucoma in VKH patients.
Electroretinogram Analysis In Patients With Non-Proliferative Diabetic Retinopathy
Authors: Maleki Zahra , Behbahani Soroor , Ahmadieh Hamid 
Published: December 2021

Abstract
Purpose: Non-proliferative diabetic retinopathy (NPDR) is the earliest stage of diabetic eye disease. Microscopic changes occur in the blood vessels of the eye in NPDR. The changes typically do not produce symptoms and are not visible to the naked eye. This paper aims to investigate a method for distinguishing NPDR based on Electroretinogram (ERG).Method: The ERG responses were recorded in 20 eyes from 14 patients with NPDR and 20 eyes from 20 healthy subjects as the control group. The responses of three standard stimuli were collected for both groups. Time-domain parameters, including amplitudes and implicit time, and a nonlinear criterion, were used to differentiate the groups. Results: This study showed that implicit time and amplitude of b-wave in dark-adapted 10.0 ERG and amplitude and implicit time of light-adapted flicker 30 Hz could distinguish between controls and NPDR groups. Theta values obtained for dark-adapted 10.0 ERG (p=0.0019), light-adapted 3.0 ERG (p=0.0021), and light-adapted flicker 30 Hz (p=0.0023) had significant differences between the groups. Conclusion: The proposed features have made it possible to distinguish between healthy and NPDR eyes. Choosing an appropriate method can effectively evaluate inner retinal dysfunction, especially in diabetic retinopathy.
Evaluating Technological Innovation Impact. An Empirical Analysis of The Offshore Wind Sector.
Authors: Shi Junguo , Hu Xuhua , Dou Shanshan , Alemzero David , Alhassan Elvis Elvis 
Published: December 2021

Abstract
This study analyses the drivers that impact innovation on offshore wind energy for a select group of countries, applying the quantile and GMM approaches for a period between 2010-2019. The OLS results from the quantile analysis say the log of trademark, Carbon emissions, offshore wind capacity, and electricity from renewable energy are significant and impact on innovation regarding offshore wind energy. Generally, the Breusch-Pagan / Cook-Weisberg test for heteroskedasticity test reveals the variables have a constant variance, confirming the robustness of the findings. The quantile regression depicts that at 25th and 75th quantiles levels, the log of trademark, the log of trade flows, the log of scientific and technical journals quantile coefficients is significantly different from zero and exhibit varied effects on the explained variable patent.Similarly, the analysis applied the IV-GMM estimation in ivreg2 to identify the over restrictions, the Hansen J statistic, and give the robust moment of conditions analysis. The findings are consistent with prior analysis with the log of trademark, the log of offshore wind capacity, the log of carbon emissions, Scientific and technology journals, the log of patent, electricity from renewables to be significant and impact on innovation.The robustness was done on the GMM models, by applying the Huber-White-Sandwich estimator of the variance of the GMM linear models approximators. The ivreg2 robust analysis revealed that the estimates are efficient for homoskedasticity and Statistics robust to heteroskedasticity.Ultimately, the interaction term ‘’cross’’ came out significant in the analysis. Signifying the importance of the interaction variables in scaling innovation.This study will sever as a reference document for policy formulators regarding scaling up innovation for offshore wind energy.
Resilience And Mental Health Among Young Adults In The Context of Lockdown Due To The COVID-19 Pandemic
Authors: Guerrero-Vaca Darío , Carpio-Arias Valeria , Rodríguez-Betancourt Andrés , Ruiz Patricio Inca , Vinueza-Veloz Andrés Fernando , Loza-Chiriboga Juan Sebastián , Pérez-Haro Jéssica , Vinueza-Veloz María Fernanda 
Published: December 2021

Abstract
Purpose: Psychological resilience is defined as the ability of an individual to adapt to adverse situations and in a certain way regulate mental health. Currently, the COVID-19 pandemic and lockdown measures implemented to avoid infection are considered risk agents for the development of mental disorders. The objective of this study is to explore the relationship between resilience capacity and the presence of depressive symptoms and poor sleep quality in the context of lockdown due to the pandemic. Methods: The sample consisted of n = 8,426 young adults who were recruited via convenience sampling. Sociodemographic and mental health data was collected through online surveys. Previously validated test were used to collect information on depression and sleep quality. Restriction of mobility was evaluated using information from Google Mobility Reports. Results: Most of participants were highly educated women. Independently from sex, age and other confounders, individuals with psychological resilience were less likely to develop depressive symptoms or sleep disorders during confinement due to the COVID-19 pandemic. Moreover, higher mobility restriction was associated with depression but not with bad sleep quality. In this way, a longest stay at home and a higher restriction of mobility to groceries and pharmacies were related to depression. Conclusions: These results show evidence of the importance of psychological resilience on mental health, and can contribute to the development of preventive public health interventions to face the negative effects of confinement on mental health.
Silicon Binding in Soil Humic Substances
Authors: kar sanjib 
Published: December 2021

Abstract
In the present study, an attempt has been made to probe the nature of silicon-humus bonding as it occurs in nature ('untreated') as compared to the samples wherefrom the metalloid ions have been removed ('treated'). Infrared (IR) spectroscopy revealed the role of carboxylic acid groups of Humic acid (HA) in metal complexation. Si-O absorption reduced considerably in treatment to remove metalloid ions, but it remained quite strong in the treated samples indicating a strong Si-O linkage with humus. In XRD analysis, treated samples showed stronger γ-bands. DTA analysis indicated structural strain on the humic molecule induced by complexation with metalloid ions. Treated HAs had higher acidity values compared to the untreated samples. Treatment for removal of metal ions caused an increase in cation exchange capacity while a reduction of E4/E6 ratio. Treated HAs also showed higher molecular weights (Mv) compared to the untreated samples. Removal of metals renders certain groups free which form intermolecular bonds. On the basis of analytical data, it was observed that there is still some silicon bonds within humic acid molecule, even after removal of silicon by forcing conditions. Association of silicon with humus in soils are quantitatively and qualitatively different from other metal ions and it is clear that humic acid molecule possess silicon-humus bond like pure organo-metallic compounds.
ROSTMEMA: A Double-Blind Randomized Placebo-Controlled Trial with the PDE4 Inhibitor Roflumilast in Patients Suffering from Long-term Cognitive Sequela After Stroke
Authors: Kerckhoffs Jill , Blokland Arjan , Prickaerts Jos , Winkens Ieke 
Published: December 2021

Abstract
Background : The aim of this study is to examine whether treatment with roflumilast improves cognition in patients suffering from cognitive sequelae after stroke. The results may provide a proof of concept on the potential of roflumilast (or other phosphodiesterase-4 (PDE-4) inhibitors) as pharmacotherapeutic treatment to enhance cognition, and will further increase our knowledge on the role of PDE4 in human cognition in general. Cognitive processes, in particular memory, will be assessed. Methods : 100 female and male patients (41-70 years old) suffering from cognitive complaints 1 year after stroke will be recruited via advertisements via social media and via local caretaking organizations. The first phase of the study will be conducted according to a double-blind, randomized placebo-controlled, between-subjects design. After a baseline measurement, participants will be tested for acute (1 hour after drug intake) and subsequent chronic (1.5 and 3 months after start treatment) treatment effects. In a second phase, the placebo group (50 people) will be given the opportunity to receive roflumilast. This is an open label design. The roflumilast group will be tested for long-term treatment effects at three months after the end of treatment. Discussion : Strengths of the current study are the open-label design as well as the assessment of long-term effects. The findings of the current study will demonstrate if the mechanism of PDE4 inhibition is a relevant target to improve cognitive functions in patients with brain damage after a stroke. If positive effects are found in this patient group, this treatment could also be relevant for other brain disorders (e.g. head trauma, mild cognitive impairment, Fragile X syndrome, schizophrenia) in which enhanced neuronal plasticity is required to improve cognition. Trial registration : The Medical Ethics Committee of Maastricht University Medical Center+ granted ethics approval of the fourth version of the protocol on February 25, 2021. The trial was registered at the European Drug Regulatory Affairs Clinical Trials (EudraCT) register on July 29, 2020, https://www.clinicaltrialsregister.eu/ctr-search/search?query=2020-003768-16 and Clinicaltrials.gov (NCT04854811) at April 21, 2021, https://clinicaltrials.gov/ct2/show/NCT04854811. The Central Committee on Research Involving Human Subjects (CCMO) granted approval on May 4, 2021.