bluuebunny/crossref_metadata_2025_split huggingface dataset:
Tracing the Long-Term Evolution of Land Cover in an Alpine Valley 1820–2015 in the Light of Climate, Glacier and Land Use Changes
Authors: Hohensinner Severin , Atzler Ulrike , Fischer Andrea , Schwaizer Gabriele , Helfricht Kay 
Published: August 2021

Alpine glacial environments and their fluvial systems are among those landscapes most comprehensively affected by climate change. Typically, studies on the consequences of climate change in such environments, e.g., glacier retreat, cover a maximum of 70 years, reflecting the availability of orthophotos or satellite images. This study addresses the long-term transformation processes in a glaciated catchment and highlights the role of human agency in a changing Alpine environment. In order to identify land cover changes between 1820 and 2015 in the Long-Term Ecosystem Research (LTER) site “Jamtal” (Tyrol, Austria) we apply a “regressive-iterative GIS reconstruction method” combining both historical maps and optical remote sensing data. Below 2,100 m a.s.l. the Jamtal experienced a massive 62% decline of unvegetated debris areas and bedrock outcrops (so-called “wasteland”) that was mainly transformed to grassland and sparsely wooded areas. Forests increased by an outstanding 323% and grassland was replaced by sparsely or densely wooded areas. This primarily reflects the abandonment of agricultural uses at unfavourable remote sites. In the higher (formerly) glaciated subbasin, ice-covered areas declined by 55%, which was associated with a major (82%) growth of exposed wasteland. Concurrently, Alpine grassland expanded by 196% and krumholz even by 304%. Approximately half of the new fluvial system that evolved in deglaciated areas between 1870 and 1921 still existed in 2015. Unconsolidated debris buried almost one fifth of the new channels, and almost one third was colonized by vegetation. Recent data show that the deglaciation process is much faster than the colonization process by Alpine vegetation. Accordingly, the extent of wasteland has expanded and potentially amplifies the sediment supply to the fluvial system. Alterations in high Alpine hydrological and sediment/debris regimes significantly affect human use in lower, more favourable areas of the Alpine region. The long-term investigation of the Alpine landscape reveals that the transformation processes have accelerated in recent decades.
Predictive Value of Serum Cytokeratin 19 Level for the Feasibility of Conserving Ovaries in Endometrial Cancer
Authors: Xu Jie , Chen Can , Xiong Jing , Wang Hui , Linghu Hua 
Published: August 2021

Objective: To determine the predictive value of cytokeratin 19 (CK19) for evaluating the safety of ovarian preservation in patients with endometrial cancer (EC).Methods: Five hundred and seventeen EC patients hospitalized from November 2010 to June 2016 were reviewed retrospectively. Pre-operative tumor biomarkers including CA125, HE4, CK19, and CA19-9 were obtained. Predictive biomarkers associated with ovarian metastasis were selected using univariate and multivariate Logistic regression. The cut-off values were determined by receiver operating characteristic (ROC) curves. Kaplan-Meier method and Cox multivariate regression model was used to perform survival analysis.Results: Among clinical parameters and biomarkers included, age &gt; 65, type II EC, CA125 ≥ 35 u/ml, CK19 &gt; 3.3 ng/ml, and myometrial invasion ≥ 50% depth appeared as significant predictors of the risk of ovarian involvement in univariable logistic analysis. In multivariable analysis, CK19 &gt; 3.3 ng/ml (OR = 11.541, 95%CI: 1.968–67.668, P = 0.007) and Type II EC (OR = 8.336, 95%CI: 1.456–47.722, P = 0.017) were independent risk predictors of ovarian metastasis in pre-menopausal women. In pre-menopausal women with Type I EC (n = 142), CK19 level could satisfactorily predict the risk of ovarian metastasis (AUC = 0.860, 95%CI: 0.792–0.912, P &lt; 0.001), and when the cut-off point was set as 2.45 ng/ml, the negative predictive value and negative likelihood ratio were 99% and 0.19, with the maximum Youden index of 0.598.Conclusions: The present study advocates the necessity of incorporating serum CK19 measurement into the pre-operative evaluation of EC, especially as extension of current standard approach with ovarian preservation counseling.
Gender Differences in Idiopathic Pulmonary Fibrosis: Are Men and Women Equal?
Authors: Sesé Lucile , Nunes Hilario , Cottin Vincent , Israel-Biet Dominique , Crestani Bruno , Guillot-Dudoret Stephanie , Cadranel Jacques , Wallaert Benoit , Tazi Abdellatif , Maître Bernard , Prévot Gregoire , Marchand-Adam Sylvain , Hirschi Sandrine , Dury Sandra , Giraud Violaine , Gondouin Anne , Bonniaud Philippe , Traclet Julie , Juvin Karine , Borie Raphael , Carton Zohra , Freynet Olivia , Gille Thomas , Planès Carole , Valeyre Dominique , Uzunhan Yurdagül 
Published: August 2021

Background: Idiopathic pulmonary fibrosis (IPF) is characterized by a male predominance. The aim of the study was to explore gender differences in a well-designed French multicentre prospective IPF cohort (COhorte FIbrose, COFI) with a 5-year follow-up.Methods: Between 2007 and 2010, 236 patients with incident IPF were included in COFI. Gender characteristics were compared using a t-test, Chi-squared test and ANOVA, as appropriate. Survival analyses were performed.Results: Fifty-one (22%) females and 185 (78%) males with an average age at diagnosis of 70.1 ± 9.20 and 67.4 ± 10.9 years, respectively, were included in the cohort. Women were significantly less exposed to tobacco smoke [never n = 32 (62.7%) vs. n = 39 (21.1%), p &lt; 0.001] and to occupational exposure [n = 7 (13.7%) vs. n = 63 (34.1%), p = 0.012]. Baseline forced vital capacity, % of predicted (FVC%) was significantly better in women compare to men (83.0% ± 25.0 v. 75.4% ± 18.7 p = 0.046). At presentation honeycombing and emphysema on CT scan were less common in women [n = 40 (78.4%) vs. n = 167 (90.3%) p = 0.041] and [n = 6 (11.8%) vs. n = 48 (25.9%) p = 0.029], respectively. During follow-up fewer women were transplanted compared to men [n = 1 (1.96%) vs. n = 20 (10.8%) p = 0.039]. Medians of survival were comparable by gender [31 months (CI 95%: 28–40) vs. 40 months (CI 95%: 33–72) p = 0.2]. After adjusting for age and FVC at inclusion, being a woman was not associated to a better survival.Conclusions: Women appear to have less advanced disease at diagnosis, maybe due to less exposure history compare to men. Disease progression and overall survival remains comparable regardless gender, but women have less access to lung transplantation.
Effects of Combined Endurance and Resistance Training in Women With Multiple Sclerosis: A Randomized Controlled Study
Authors: Correale Luca , Buzzachera Cosme Franklim , Liberali Giulia , Codrons Erwan , Mallucci Giulia , Vandoni Matteo , Montomoli Cristina , Bergamaschi Roberto 
Published: August 2021

Purpose: To test the hypothesis that combined resistance and endurance training would improve muscle strength, fatigue, depression, and quality of life in persons with MS.Methods: Twenty-seven women with MS were randomly assigned to either control (CON, n = 13) or the experimental (EXP, n = 14) group. The participants in the EXP group trained twice a week for 12 weeks, followed by 12 weeks of detraining. Both CON and EXP groups were tested before and after 12 weeks of the intervention period, as well as 12 weeks after training cessation (follow-up), where measures of muscle strength, fatigue, depression, and quality of life were evaluated.Results: There were significant changes in maximal voluntary isometric contraction (MVIC), 1RM leg extension, and 1RM chest press following the intervention period in the EXP group (P &lt; 0.05), but not in the CON group (P &gt; 0.05). These changes persisted after 12 weeks of detraining. Similar findings were found for fatigue, depression, and physical and mental composites of quality of life.Conclusion: These results suggest that combined exercise training, at a minimum, prevents the disease-related deterioration of muscular performance and quality of life and well-being in persons with MS.
Dosimetric Comparison, Treatment Efficiency Estimation, and Biological Evaluation of Popular Stereotactic Radiosurgery Options in Treating Single Small Brain Metastasis
Authors: Duan Yanhua , Cao Hongbin , Wu Boheng , Wu Yinghui , Liu Dong , Zhou Lijun , Feng Aihui , Wang Hao , Chen Hua , Gu Hengle , Shao Yan , Huang Ying , Lin Yang , Ma Kui , Fu Xiaolong , Fu Hong , Kong Qing , Xu Zhiyong 
Published: August 2021

ObjectivesThis study aimed to show the advantages of each stereotactic radiosurgery (SRS) treatment option for single small brain metastasis among Gamma Knife (GK), Cone-based VMAT (Cone-VMAT), and MLC-based CRT (MLC-CRT) plans.Materials and MethodsGK, Cone-VMAT, and MLC-CRT SRS plans were retrospectively generated for 11 patients with single small brain metastasis whose volume of gross tumor volume (GTV) ranged from 0.18 to 0.76 cc (median volume 0.60 cc). Dosimetric parameters, treatment efficiency, and biological parameters of the three techniques were compared and evaluated. The metric variation with the planning target volume (PTV) was also studied.ResultsThe conformity index (CI) was similar in GK and MLC-CRT plans, higher than Cone-VMAT. Cone-VMAT achieved comparable volume covered by 12 Gy (V12) and gradient index (GI) as GK, lower than MLC-CRT. The heterogeneity index (HI) of GK, Cone-VMAT, and MLC-CRT decreased sequentially. GK gave the lowest volume covered by 3 Gy (V3) and 6 Gy (V6), while MLC-CRT got the highest. The beam-on time and treatment time of GK, Cone-VMAT, and MLC-CRT decreased in turn. Tumor control probability (TCP) of all three SRS plans was greater than 98%, and normal tissue complication probability (NTCP) of all organs at risk (OARs) was below 0.01%. GK and Cone-VMAT resulted in superior TCP and NTCP of the normal brain tissue than MLC-CRT. The relative value of Cone-VMAT and GK for all metrics hardly changed with the target volume. Except for the unchanged HI and TCP, the other results of MLC-CRT with respect to GK improved as the target volume increased. MLC-CRT could produce higher CI than GK and Cone-VMAT when the target volume increased above 2 and 1.44 cc, respectively.ConclusionFor single small brain metastases, Cone-VMAT may be used as an alternative to GK-free centers. In addition to the advantage of short treatment time, MLC-CRT showed superiority in CI as the target volume increased. Treatment centers can choose appropriate SRS technique on a case-by-case basis according to institutional conditions and patients’ individual needs.
Disappearance of Seasonal Respiratory Viruses in Children Under Two Years Old During COVID-19 Pandemic: A Monocentric Retrospective Study in Milan, Italy
Authors: Ippolito Giulio , La Vecchia Adriano , Umbrello Giulia , Di Pietro Giada , Bono Patrizia , Scalia Catenacci Stefano , Pinzani Raffaella , Tagliabue Claudia , Bosis Samantha , Agostoni Carlo , Marchisio Paola Giovanna 
Published: August 2021

Background: The containment measures adopted during COVID-19 pandemic have influenced the epidemiology of other respiratory viruses.Aim: We analyzed the modification of the incidence and etiology of lower respiratory tract infections (LRTIs) in young children during COVID-19 pandemic.Methods: Case series of all children under 2 years old hospitalized at a tertiary care Hospital in the Center of Milan, Italy diagnosed with LRTIs in three consecutive winter seasons (from the 1st of November to the last day of February in 2018/2019, 2019/2020 and 2020/2021). We compared the number of hospitalizations and viral detections in the 2020/2021 with the average of 2018/2019 and 2019/2020 (pre-COVID-19) using the Poisson distribution.Results: we enrolled 178 patients (66 from 2018/2019, 96 from 2019/2020, 16 from 2020/2021) 94 males (53%) and 84 females (47%), with a median (IQR) age of 5 (2–13) months. The number of hospitalizations during the 2020/2021 season was 80% lower than the average of the pre-COVID-19 seasons (16 vs. 81, p&lt;0.001). Overall, 171 (96%) patient's nasopharyngeal aspirate (NPA) detected at least one virus (110, 64%, single-detection, 61, 36%, co-detections). In 2020/2021 we observed the disappearance of Respiratory Syncytial virus (0 vs. 54, p &lt; 0.001), Influenza virus (0 vs. 6.5, p = 0.002), Metapneumovirus (0 vs. 8, p &lt; 0.001), Parainfluenza viruses (0 vs. 3.5, p = 0.03) and a significant reduction of Adenovirus (2 vs. 7, p = 0.03), Bocavirus (2 vs. 7.5, p = 0.02) and Enterovirus (1 vs. 5, p = 0.04). No significant difference was found for Rhinoviruses (14 cases vs. 17, p = 0.2), other Coronaviruses (0 vs. 2, p = 0.1), and Cytomegalovirus (1 vs. 1, p = 0.7).Conclusions: We observed a striking reduction in hospitalizations due to LRTIs and a modification of the etiology, with enveloped viruses mainly affected.
Milk Fat Globule-EGF Factor 8 Alleviates Pancreatic Fibrosis by Inhibiting ER Stress-Induced Chaperone-Mediated Autophagy in Mice
Authors: Ren Yifan , Cui Qing , Zhang Jia , Liu Wuming , Xu Meng , Lv Yi , Wu Zheng , Zhang Yuanyuan , Wu Rongqian 
Published: August 2021

Pancreatic fibrosis is an important pathophysiological feature of chronic pancreatitis (CP). Our recent study has shown that milk fat globule-EGF factor 8 (MFG-E8) is beneficial in acute pancreatitis. However, its role in CP remained unknown. To study this, CP was induced in male adult Mfge8-knockout (Mfge8-KO) mice and wild type (WT) mice by six intraperitoneal injections of cerulein (50 μg/kg/body weight) twice a week for 10 weeks. The results showed that knockout of mfge8 gene aggravated pancreatic fibrosis after repeated cerulein injection. In WT mice, pancreatic levels of MFG-E8 were reduced after induction of CP and administration of recombinant MFG-E8 alleviated cerulein-induced pancreatic fibrosis. The protective effect of MFG-E8 in CP was associated with reduced autophagy and oxidative stress. In human pancreatic stellate cells (PSCs), MFG-E8 inhibited TGF-β1-induced ER stress and autophagy. MFG-E8 downregulated the expression of lysosomal associated membrane protein 2A (LAMP2A), a key factor in ER stress-induced chaperone-mediated autophagy (CMA). QX77, an activator of CMA, eliminated the effects of MFG-E8 on TGF-β1-induced PSC activation. In conclusion, MFG-E8 appears to mitigate pancreatic fibrosis via inhibiting ER stress-induced chaperone-mediated autophagy. Recombinant MFG-E8 may be developed as a novel treatment for pancreatic fibrosis in CP.
Long-Lasting Changes in Glial Cells Isolated From Rats Subjected to the Valproic Acid Model of Autism Spectrum Disorder
Authors: Traetta Marianela Evelyn , Uccelli Nonthué Alejandra , Zárate Sandra Cristina , Gómez Cuautle Dante , Ramos Alberto Javier , Reinés Analía 
Published: August 2021

Synaptic alterations concomitant with neuroinflammation have been described in patients and experimental models of autism spectrum disorder (ASD). However, the role of microglia and astroglia in relation to synaptic changes is poorly understood. Male Wistar rats prenatally exposed to valproic acid (VPA, 450 mg/kg, i.p.) or saline (control) at embryonic day 10.5 were used to study synapses, microglia, and astroglia in the prefrontal cortex (PFC) at postnatal days 3 and 35 (PND3 and PND35). Primary cultures of cortical neurons, microglia, and astroglia isolated from control and VPA animals were used to study each cell type individually, neuron-microglia and microglia-astroglia crosstalk. In the PFC of VPA rats, synaptic changes characterized by an increase in the number of excitatory synapses were evidenced at PND3 and persisted until PND35. At PND3, microglia and astroglia from VPA animals were morphologically similar to those of age-matched controls, whereas at PND35, reactive microgliosis and astrogliosis were observed in the PFC of VPA animals. Cortical neurons isolated from VPA rats mimicked in vitro the synaptic pattern seen in vivo. Cortical microglia and astroglia isolated from VPA animals exhibited reactive morphology, increased pro-inflammatory cytokines, and a compromised miRNA processing machinery. Microglia from VPA animals also showed resistance to a phagocytic challenge. In the presence of neurons from VPA animals, microglia isolated from VPA rats revealed a non-reactive morphology and promoted neurite outgrowth, while microglia from control animals displayed a reactive profile and promoted dendritic retraction. In microglia-astroglia co-cultures, microglia from VPA animals displayed a reactive profile and exacerbated astrocyte reactivity. Our study indicates that cortical microglia from VPA animals are insensitive or adapted to neuronal cues expressed by neurons from VPA animals. Further, long-term in vivo microgliosis could be the result of altered microglia-astroglia crosstalk in VPA animals. Thus, our study highlights cortical microglia-astroglia communication as a new mechanism implicated in neuroinflammation in ASD; consequently, we propose that this crosstalk is a potential target for interventions in this disorder.
Sinomenine Attenuated Capsaicin-Induced Increase in Cough Sensitivity in Guinea Pigs by Inhibiting SOX5/TRPV1 Axis and Inflammatory Response
Authors: Ma Jian-Ling , Ji Kun , Shi Li-Qing , Li Niu-Niu , Wang Li-Yun , Dong Shang-Juan , Zhang Yan-Xia , Wen Shao-Hui , Liu Xue-Mei , Wang Ying , Luo Jing-Yue 
Published: August 2021

BackgroundChronic cough is a common complaint which affects a large number of patients worldwide. Increased cough sensitivity is a very important cause of chronic persistent cough. However, there are limited clinical diagnosis and treatment for increased cough sensitivity. Transient receptor potential vanilloid-1 (TRPVl) is a member of the transient receptor potential (TRP) family of channels which is very closely associated with respiratory diseases. However, the mechanism through which TRPV1 that influences downstream events is still poorly understood.ResultsCapsaicin induced increase in cough sensitivity by upregulating the protein level of TRPV1, leading to the secretions of Substance P and neurokinin A which stimulated neurogenic inflammation. However, sinomenine, a component of traditional Chinese medicine, significantly attenuated the capsaicin-induced cough by inhibiting the expression of TRPV1 in guinea pigs. In addition, capsaicin increased the expression of SOX5 which mediated the transcriptional upregulation of TRPV1. However, pretreatment with sinomenine reduced the expression of SOX5.ConclusionThese results indicate that capsaicin induced increase in cough sensitivity by activating neurogenic inflammation, while sinomenine attenuated the increase in cough sensitivity by inhibiting the expressions of SOX5 and TRPV1 in guinea pigs. This finding may provide a novel target for the treatment of aggravated cough sensitivity.
The EURO-D Measure of Depressive Symptoms in the Aging Population: Comparability Across European Countries and Israel
Authors: Maskileyson Dina , Seddig Daniel , Davidov Eldad 
Published: August 2021

Most of the countries in Europe are experiencing a rapid aging of their populations and with this an increase in mental health challenges due to aging. Comparative research may help countries to assess the promotion of healthy aging in general, and mentally healthy aging in particular, and explore ways for adapting mental health policy measures. However, the comparative study of mental health indicators requires that the groups understand the survey questions inquiring about their mental health in the same way and display similar response patterns. Otherwise, observed differences in perceived mental health may not reflect true differences but rather cultural bias in the health measures. To date, research on cross-country equivalence of depression measures among older populations has received very limited attention. Thus, there is a growing need for the cross-country validation of existing depression measures using samples of the older population and establishing measurement equivalence of the assessment tools. Indeed, insights on mental health outcomes and how they compare across societies is paramount to inform policy makers seeking to improve mental health conditions of the populations. This study, therefore, aims to examine measurement equivalence of self-reported depressive symptoms among older populations in 17 European countries and Israel. The data for the current analysis are from the sixth wave (2015) of the Survey on Health, Ageing and Retirement in Europe (SHARE) and consist of the population of respondents 50 years of age and older. The measurement of depression is based on the EURO-D scale, which was developed by a European consortium. It identifies existing depressive symptoms and consists of the 12 items: depression, pessimism, suicidality, guilt, sleep, interest, irritability, appetite, fatigue, concentration, enjoyment, and tearfulness. We examine the cross-country comparability of these data by testing for measurement equivalence using multigroup confirmatory factor analysis (MGCFA) and alignment. Our findings reveal partial equivalence thus allowing us to draw meaningful conclusions on similarities and differences among the older population across 18 countries on the EURO-D measure of depression. Findings are discussed in light of policy implications for universal access to mental health care across countries.
Differential Effects of Ethical Education, Physical Hatha Yoga, and Mantra Meditation on Well-Being and Stress in Healthy Participants—An Experimental Single-Case Study
Authors: Matko Karin , Sedlmeier Peter , Bringmann Holger C. 
Published: August 2021

Traditionally, yoga is a multicomponent practice consisting of postures, breathing techniques, meditation, mantras, and ethics. To date, only a few studies have tried to dismantle the effects of each of these components and their combinations. To fill this gap, we examined the incremental effects of ethical education and physical Hatha yoga on mantra meditation using a single-case multiple-baseline design. This study was part of a project evaluating the new mind–body programMeditation-Based Lifestyle Modification. Fifty-seven healthy participants with no regular yoga or meditation practice were randomly assigned to three baselines (7, 14, and 21 days) and four conditions using a random number generator. The conditions were mantra meditation alone (MA), meditation plus physical yoga (MY), meditation plus ethical education (ME), and meditation plus yoga and ethical education (MYE). All the interventions lasted for 8 weeks and were run consecutively according to baseline length. During the baseline and treatment phases, participants received daily questionnaires measuring their well-being (WHO-5 Well-Being Index), stress (Perceived Stress Scale), and subjective experiences. Forty-two participants completed the treatment and were entered in the analyses. We analyzed our data using visual inspection, effect size estimation (Tau-U), and multilevel modeling. Almost all participants showed a longitudinal increase in well-being. Regarding between-group differences, participants who received ethical education exhibited the largest increases in well-being (Tau-U= 0.30/0.23 for ME/MYE), followed by participants in the MY condition (Tau-U= 0.12). Conversely, participants in the MA condition showed no change (Tau-U= 0.07). There was a tendency for the combined treatments to decrease stress. This tendency was strongest in the MY condition (Tau-U= –0.40) and reversed in the MA condition (Tau-U= 0.17). These results emphasize the incremental and differential effects of practicing meditation in combination with other practices from the eight-fold yoga path. This approach is valuable for better understanding the multifaceted practice of yoga.Clinical Trial Registration:www.ClinicalTrials.gov, identifier: NCT04252976.