bluuebunny/crossref_metadata_2025_split huggingface dataset:
Matrix Normalization Techniques for Definitive Urine Drug Testing
Authors: Rosano Thomas G , Rumberger John M , Wood Michelle 
Published: May 2021

Abstract
Analytical performance of stable isotope-labeled internal standardization (SIL-IS) and threshold accurate calibration (TAC) methods of matrix normalization are compared for quantitation of 51 drugs and metabolites (analytes) in urine with analysis by ultra performance liquid chromatography with tandem mass spectrometry (UPLC–MS-MS). Two SIL-IS methods of analysis were performed, one method using analyte-specific internal standardization (ASIL-IS) and another method using a shared stable isotope from another analyte for internal standardization (SSIL-IS). Variance in inter-specimen matrix effect, without the use of a matrix normalization method, was studied by UPLC–MS-MS analysis of 338 urine donor samples and showed &gt;200% variation in ion response for some analytes. Matrix normalization methods were evaluated for precision, accuracy, calibration, multi-matrix recovery and positive casework quantitation. Acceptable calibration and quality control criteria were achieved for all methods when calibrators and controls were prepared from the same urine matrix pool. Quantitative accuracy, determined by the addition of analytes to multi-donor urine pools at two concentration levels, resulted in acceptable percent relative standard deviation (%RSD) and bias for TAC and ASIL-IS methods. SSIL-IS method quantitations in analyte-supplemented donor pools revealed a %RSD ranging from 20% to 60% for &gt;30% of the analytes and a method bias that ranged up to 87%, with a differential matrix effect on analyte and shared internal standard accounting for the imprecision and bias. Analyte quantitation in 162 authentic case samples showed close agreement for TAC and ASIL-IS methods, with greater variance in the SSIL-IS method. The study demonstrates effective matrix normalization by ASIL-IS and TAC methods and a matrix-caused bias in the SSIL-IS method.
Use of Ground Covers to Control Three-Cornered Alfalfa Hopper, Spissistilus festinus (Hemiptera: Membracidae), and Other Suspected Vectors of Grapevine Red Blotch Virus
Authors: Billings Alexis C , Flores Kristen , McCalla Kelsey A , Daane Kent M , Wilson Houston 
Published: May 2021

Abstract
Grapevine red blotch virus (GRBV) is the causal agent of grapevine red blotch disease, which affects wine grapes and leads to reduced crop yield and quality. The pathogen-plant-vector relationship of GRBV is not well understood; however, some possible vectors have been identified: Caladonus coquilletti (Van Duzee; Hemiptera: Cicadellidae), Colladonus reductus (Van Duzee; Hemiptera: Cicadellidae), Erythroneura spp., Melanoliarus sp. (Hemiptera: Cixiidae), Osbornellus borealis DeLong. & Mohr (Hemiptera: Cicadellidae), Scaphytopius granticus (Ball; Hemiptera: Cicadellidae), Spissistilus festinus (Say). Of these species, S. festinus has been shown to transmit the virus to uninfected grapevines, making it of particular interest. Since the pathogen-plant-vector relationship of GRBV is not yet completely understood, pesticide use is not necessarily the best way to manage these possible vectors. Here we test if ground cover removal, by discing in spring, could reduce the activity of potential GRBV vectors. We show that S. festinus presence in the canopy was reduced in disc rows compared to just mowing the ground vegetation, whereas there were no differences in presence in the canopy between disc and mow rows of the other possible insect vectors. Erythroneura elegantula (Osborn; Hemiptera: Cicadellidae), a common pest of grapevines but not a candidate GRBV vector, was found to have higher densities in the canopy in disc rows compared to mow rows, an effect possibly mediated by changes in vine vigor associated with ground covers. We conclude that if S. festinus is a primary vector of GRBV, discing ground covers in early spring may be a viable way to reduce their presence in the vine canopy.
A Regional Approach Shows Differences Among Invasive AntsSolenopsis geminataandWasmannia auropunctata(Hymenoptera: Formicidae) Within Its Native Range of Distribution
Authors: Rojas Patricia , Fragoso Carlos 
Published: May 2021

AbstractWorldwide, two of the most harmful invasive ants typical of disturbed sites are Solenopsis geminata (Fabricius) and Wasmannia auropunctata (Roger). Both are natives of the Neotropics and are widely distributed all over the tropics. Within its original geographic range, there are few data on its abundance and potential damage to natural ecosystems. In this study, we recorded their abundance and relationships to diversity and richness of soil ant communities in two localities with different amount of forested area (López Mateos, LM 77% and Venustiano Carranza, VC 27%), at Los Tuxtlas reserve. In each locality, four land use systems (LUS) were sampled: tropical rain forests, agroforestry plantations, annual crops, and pastures. Data were gathered from 360 ant samples obtained from litter squares, pitfall traps, and soil monoliths in 40 sampling points (20 per locality, and five per LUS). Solenopsis geminata was more abundant in LM than in VC; the opposite trend was observed for W. auropunctata. In LM, S. geminata was more abundant in crops than in the other LUS, whereas W. auropunctata tended to have higher abundances in less managed sites of both localities. Abundance and species richness of ant communities were higher in LM than in VC. At regional and local levels, we found negative relationships between the abundance of S. geminata and species richness; the inverse pattern was found for W. auropunctata. We conclude that at Los Tuxtlas, W. auropunctata can be considered as a typical dominant native species, whereas S. geminata is the common exotic invasive ant.
Incidence of CMV Infection and Disease and Adverse Events Associated with Antiviral Therapy in a Retrospective Cohort of Allogeneic Hematopoietic Cell Transplant Recipients at an Academic Children’s Hospital
Authors: Hayes Molly , Newman Alexander M , Boge Craig L K , Galetaki Despoina M , Elgarten Caitlin W , Freedman Jason L , Olson Timothy S , Fisher Brian T 
Published: May 2021

AbstractBackgroundCytomegalovirus (CMV) is a significant source of morbidity and mortality among transplant recipients; the epidemiology is less understood in pediatric hematopoietic cell transplantation (HCT) cohorts. Furthermore, there is a paucity of data related to CMV prophylactic and preemptive strategies.MethodsA single-center retrospective observational cohort of allogeneic HCT recipients at the Children’s Hospital of Philadelphia January 1, 2004–December 31, 2017 was constructed. Subjects were followed for 180 days after transplant to determine whether they had CMV infection or disease. Data on antiviral therapy were collected as were outcomes of CMV disease and adverse events (AEs) related to the antiviral therapy.ResultsBetween January 2004 and March 2017, 345 allogeneic HCTs in 333 patients undergoing CMV surveillance testing were identified. CMV DNAemia was detected during the 180-day follow-up in 89 (25.8%) HCTs. CMV recipient-positive transplants were most likely to have CMV infection (47%). Infection rates were high for those receiving a CMV-specific prophylaxis regimen (50%). CMV DNAemia progressed to CMV disease 11.2% of the time. Of 224 subjects receiving CMV-specific prophylaxis, 19.2% experienced ≥1 AE. Of 53 receiving preemptive therapy during any CMV DNAemia episode, 32.1% experienced ≥1 AE.ConclusionsCMV infection is common in pediatric allogeneic HCT recipients. The CMV-specific prophylaxis regimen employed in this cohort did not effectively prevent DNAemia, progression to CMV disease was uncommon, and AEs from prophylaxis and preemptive therapy were frequent. Novel approaches that reduce the impact of CMV on pediatric allogeneic HCT recipients are needed.
Insights into the role of alternative splicing in plant temperature response
Authors: Dikaya Varvara , El Arbi Nabila , Rojas-Murcia Nelson , Nardeli Sarah Muniz , Goretti Daniela , Schmid Markus 
Published: May 2021

AbstractAlternative splicing occurs in all eukaryotic organisms. Since the first description of multiexon genes and the splicing machinery, the field has expanded rapidly, especially in animals and yeast. However, our knowledge about splicing in plants is still quite fragmented. Though eukaryotes show some similarity in the composition and dynamics of their splicing machinery, observations of unique plant traits are only starting to emerge. For instance, plant alternative splicing is closely linked to their ability to perceive various environmental stimuli. Due to their sessile lifestyle, temperature is a central source of information, allowing plants to adjust their development to match current growth conditions. Hence, seasonal temperature fluctuations and day–night cycles can strongly influence plant morphology across developmental stages. Here we discuss available data on temperature-dependent alternative splicing in plants. Given its fragmented state, it is not always possible to fit specific observations into a coherent picture, yet it is sufficient to estimate the complexity of this field and the need for further research. Better understanding of alternative splicing as a part of plant temperature response and adaptation may also prove to be a powerful tool for both fundamental and applied sciences.
Unveiling the singular dynamics in the cosmic large-scale structure
Authors: Rampf Cornelius , Frisch Uriel , Hahn Oliver 
Published: May 2021

ABSTRACT
It is known that the gravitational collapse of cold dark matter leads to infinite-density caustics that seed the primordial dark-matter haloes in the large-scale structure. The development of these caustics begins, generically, as an almost one-dimensional phenomenon with the formation of pancakes. Focusing on the one-dimensional case, we identify a landscape of non-differentiable, and thus, singular features in the particle acceleration that emerge after the first crossing of particle trajectories. We complement our fully analytical studies by high-resolution simulations and find outstanding agreement, particularly shortly after the first crossing. We develop the methods in one space dimension but outline briefly the necessary steps for the 3D case.
Prostate cancer-associated SPOP mutations lead to genomic instability through disruption of the SPOP–HIPK2 axis
Authors: Jin Xiaofeng , Qing Shi , Li Qian , Zhuang Hui , Shen Liliang , Li Jinhui , Qi Honggang , Lin Ting , Lin Zihan , Wang Jian , Cao Xinyi , Yang Jianye , Ma Qi , Cong Linghua , Xi Yang , Fang Shuai , Meng Xiaodan , Gong Zhaohui , Ye Meng , Wang Shuyun , Wang Chenji , Gao Kun 
Published: May 2021

Abstract
Speckle-type Poz protein (SPOP), an E3 ubiquitin ligase adaptor, is the most frequently mutated gene in prostate cancer. The SPOP-mutated subtype of prostate cancer shows high genomic instability, but the underlying mechanisms causing this phenotype are still largely unknown. Here, we report that upon DNA damage, SPOP is phosphorylated at Ser119 by the ATM serine/threonine kinase, which potentiates the binding of SPOP to homeodomain-interacting protein kinase 2 (HIPK2), resulting in a nondegradative ubiquitination of HIPK2. This modification subsequently increases the phosphorylation activity of HIPK2 toward HP1γ, and then promotes the dissociation of HP1γ from trimethylated (Lys9) histone H3 (H3K9me3) to initiate DNA damage repair. Moreover, the effect of SPOP on the HIPK2-HP1γ axis is abrogated by prostate cancer-associated SPOP mutations. Our findings provide new insights into the molecular mechanism of SPOP mutations-driven genomic instability in prostate cancer.
Cropland redistribution to marginal lands undermines environmental sustainability
Authors: Kuang Wenhui , Liu Jiyuan , Tian Hanqin , Shi Hao , Dong Jinwei , Song Changqing , Li Xiaoyong , Du Guoming , Hou Yali , Lu Dengsheng , Chi Wenfeng , Pan Tao , Zhang Shuwen , Hamdi Rafiq , Yin Zherui , Yan Huimin , Yan Changzhen , Wu Shixin , Li Rendong , Yang Jiuchun , Dou Yinyin , Wu Wenbin , Liang Liqiao , Xiang Bao , Yang Shiqi 
Published: May 2021

ABSTRACT
Cropland redistribution to marginal land has been reported worldwide; however, the resulting impacts on environmental sustainability have not been investigated sufficiently. Here we investigated the environmental impacts of cropland redistribution in China. As a result of urbanization-induced loss of high-quality croplands in south China (∼8.5 t ha–1), croplands expanded to marginal lands in northeast (∼4.5 t ha–1) and northwest China (∼2.9 t ha–1) during 1990–2015 to pursue food security. However, the reclamation in these low-yield and ecologically vulnerable zones considerably undermined local environmental sustainability, for example increasing wind erosion (+3.47%), irrigation water consumption (+34.42%), fertilizer use (+20.02%) and decreasing natural habitats (−3.11%). Forecasts show that further reclamation in marginal lands per current policies would exacerbate environmental costs by 2050. The future cropland security risk will be remarkably intensified because of the conflict between food production and environmental sustainability. Our research suggests that globally emerging reclamation of marginal lands should be restricted and crop yield boost should be encouraged for both food security and environmental benefits.
Interventions to Treat Obesity in Mexican Children and Adolescents: Systematic Review and Meta-Analysis
Authors: Aceves-Martins Magaly , López-Cruz Lizet , García-Botello Marcela , Gutierrez-Gómez Yareni Yunuen , Moreno-García Carlos Francisco 
Published: May 2021

Abstract

Context
Prevalence of overweight and obesity has been rising in the past 3 decades among Mexican children and adolescents.

Objective
To systematically review experimental studies evaluating interventions to treat obesity in Mexican children and adolescents (≤18 years old).

Data Sources
For this study, 13 databases and 1 search engine were searched.

Data Analysis
A total of 29 studies met the inclusion criteria. Overall, 2302 participants (age range, 8–16 years) from 11 states in Mexico were included. Most of the studies (n = 17 of 29) were provided in a clinical setting. A random-effect meta-analysis of 4 randomized controlled trials was conducted and a significant effect was found on body mass index reduction that favored the intervention group (−1.52; 95%CI, −2.15 to −0.89) for short-term (≤6 mo) interventions.

Conclusions
A multicomponent, multidisciplinary, and individualized intervention that includes dietary modifications, physical activity practice, behavioral strategies, and active parental involvement might help treat childhood obesity in Mexico. However, long-term results need to be produced to identify effectiveness pointers that might help establish an integrated, long-lasting care model to treat obesity.
An Elementary Theory of Directed Technical Change and Wage Inequality
Authors: Loebbing Jonas 
Published: May 2021

AbstractThis article generalizes central results from the theory of (endogenously) directed technical change to settings where technology does not take a labour-augmenting form and with arbitrarily many levels of skill. Building on simple notions of complementarity, the results remain intuitive despite their generality. The developed theory allows to study the endogenous determination of labour-replacing, that is, automation technology through the lens of directed technical change theory. In an assignment model with a continuum of differentially skilled workers and capital, where capital perfectly substitutes for labour in the production of tasks, any increase in the relative supply of skilled workers stimulates investment into improving the productivity of capital, potentially leading skill premia to increase in relative skill supply. Relatedly, trade with a skill-scarce country discourages improvements in capital productivity, potentially reversing the standard Heckscher–Ohlin effects.
‘To Cause Sleepe Safe and Shure’: Dangerous Substances, Sleep Medicine and Poison Theories in Early Modern England
Authors: Hunter Elizabeth K 
Published: May 2021

Summary
Recipes found in letters and manuscript receipt books testify to the use of potentially lethal substances in domestic sleep medicine. This article examines the theory behind the use of poisons to induce sleep, contrasting Galenic theory with the radical approach of the Paracelsians. According to Galenic medicine, the coldness of stupefactives such as henbane, deadly nightshade and the opium poppy were useful in counteracting fever and helping a patient to sleep. However, their coldness could also cause death. They were therefore used mainly in external medicine. The exceptions were diacodium made from native poppies that were considered less lethal, and sleeping draughts used in a surgical context. Laudanum, a new drug developed using alchemical methods to separate medicine from poison, broke with traditional safety advice. On account of its novelty, personal experience and recommendation were particularly important in establishing it within the canon of sleeping drugs considered safe for use.
Predicting circadian phase across populations: a comparison of mathematical models and wearable devices
Authors: Huang Yitong , Mayer Caleb , Cheng Philip , Siddula Alankrita , Burgess Helen J , Drake Christopher , Goldstein Cathy , Walch Olivia , Forger Daniel B 
Published: May 2021

AbstractFrom smart work scheduling to optimal drug timing, there is enormous potential in translating circadian rhythms research results for precision medicine in the real world. However, the pursuit of such effort requires the ability to accurately estimate circadian phase outside of the laboratory. One approach is to predict circadian phase noninvasively using light and activity measurements and mathematical models of the human circadian clock. Most mathematical models take light as an input and predict the effect of light on the human circadian system. However, consumer-grade wearables that are already owned by millions of individuals record activity instead of light, which prompts an evaluation of the accuracy of predicting circadian phase using motion alone. Here, we evaluate the ability of four different models of the human circadian clock to estimate circadian phase from data acquired by wrist-worn wearable devices. Multiple datasets across populations with varying degrees of circadian disruption were used for generalizability. Though the models we test yield similar predictions, analysis of data from 27 shift workers with high levels of circadian disruption shows that activity, which is recorded in almost every wearable device, is better at predicting circadian phase than measured light levels from wrist-worn devices when processed by mathematical models. In those living under normal living conditions, circadian phase can typically be predicted to within 1 h, even with data from a widely available commercial device (the Apple Watch). These results show that circadian phase can be predicted using existing data passively collected by millions of individuals with comparable accuracy to much more invasive and expensive methods.
A systematic review of patient-reported outcome measures used to assess sleep in postpartum women using Consensus Based Standards for the Selection of Health Measurement Instruments (COSMIN) guidelines
Authors: Sultan P , Ando K , Sultan E , Hawkins J , Blake L , Barwick F , Kawai M , Carvalho B 
Published: May 2021

AbstractStudy ObjectivesWe performed a systematic review to identify the best patient-reported outcome measure (PROM) of postpartum sleep in women.MethodsWe searched four databases for validated PROMs used to assess postpartum sleep. Studies were considered if they evaluated at least one psychometric measurement property of a PROM. An overall performance rating was assigned for each psychometric measurement property of each PROM based upon COSMIN criteria. A modified GRADE approach was used to assess the level of evidence and recommendations were then made for each PROM.ResultsWe identified 15 validation studies of eight PROMs, in 9,070 postpartum women. An adequate number of sleep domains was assessed by five PROMs: Bergen Insomnia Scale (BIS), Pittsburgh Sleep Quality Index (PSQI), General Sleep Disturbance Scale (GSDS), Athens Insomnia Scale (AIS), and the Sleep Symptom Checklist (SSC). BIS and GSDS were the only PROMs to demonstrate adequate content validity and at least a low level of evidence of sufficient internal consistency, resulting in Class A recommendations. The BIS was the only PROM, which is easily accessible and free to use for noncommercial research, that achieved a Class A recommendation.ConclusionThe BIS is the best currently available PROM of postpartum sleep. However, this PROM fails to assess several important domains such as sleep duration (and efficiency), chronotype, sleep-disordered breathing and medication usage. Future studies should focus on evaluating the psychometric measurement properties of BIS in the North American setting and in different cultural groups, or to develop a more specific PROM of postpartum sleep.
Overnight affective dynamics and sleep characteristics as predictors of depression and its development in women
Authors: Minaeva Olga , George Sandip V , Kuranova Anna , Jacobs Nele , Thiery Evert , Derom Catherine , Wichers Marieke , Riese Harriëtte , Booij Sanne H 
Published: May 2021

Abstract

Study Objectives
We examined (1) differences in overnight affective inertia (carry-over of evening affect to the next morning) for positive (PA) and negative affect (NA) between individuals with past, current, and no depression; (2) how sleep duration and quality influence overnight affective inertia in these groups, and (3) whether overnight affective inertia predicts depression development.

Methods
We used data of 579 women from the East-Flanders Prospective Twin Survey. For aim 1 and 2, individuals with past (n = 82), current (n = 26), and without (lifetime) depression (n = 471) at baseline were examined. For aim 3, we examined individuals who did (n = 58) and did not (n = 319) develop a depressive episode at 12-month follow-up. Momentary PA and NA were assessed 10 times a day for 5 days. Sleep was assessed daily with sleep diaries. Affective inertia was operationalized as the influence of evening affect on morning affect. Linear mixed-effect models were used to test the hypotheses.

Results
Overnight affective inertia for NA was significantly larger in the current compared to the non-depressed group, and daytime NA inertia was larger in the past compared to the non-depressed group. Overnight NA inertia was differently associated with shorter sleep duration in both depression groups and with lower sleep quality in the current compared to the non-depressed group. Overnight affective inertia did not predict depression development at 12-month follow-up.

Conclusions
Current findings demonstrate the importance of studying complex affect dynamics such as overnight affective inertia in relation to depression and sleep characteristics. Replication of these findings, preferably with longer time-series, is needed.