bluuebunny/crossref_metadata_2025_split huggingface dataset:
Dispariflora robertae gen. et sp. nov., a mid-Cretaceous flower of possible Lauralean affinity from Myanmar amber
Authors: Poinar, Jr. George O. , Chambers Kenton L. 
Published: November 2021

Three flowers of a fossil angiosperm preserved in amber from Myanmar (Burma) are described as the new genus and species Dispariflora robertae. Although joined in a single cymose inflorescence, the flowers are variable in size and, in addition, they possess an unusual perianth in which 1 sepal is much enlarged and leaf-like, while the remaining 4 are smaller and unequal. The flowers each have 1 superior pistil with a peculiarly plumose and bristly ovary. The number of stamens is uncertain because most were lost before floral preservation, but scars on the receptacle suggest that at least 15 were present. The bithecal anthers open by longitudinal slits and basal glands may have been present on the filaments. Taken as a whole, the floral features that characterize Dispariflora suggest an affinity with members of Laurales, especially several Southern Hemisphere families allied with the Monimiaceae.
Miconia rufinervis (Melastomataceae: Miconieae), an enigmatic endemic of the Massif de la Hotte, Haiti
Authors: Judd Walter S. 
Published: November 2021

An updated description of this enigmatic species, an endemic to the Massif de la Hotte, Haiti, is provided, and the presence of axillary inflorescences is reported for the first time. Miconia rufinervis likely belongs within the Caribbean clade of Miconieae; however, its placement within this clade is problematic, although it does show some features of Miconia sect. Calycopteris. Axillary inflorescences are present in M. rufinervis, although these are quite uncommon within the Caribbean clade. Finally, the species is distinctive in the morphology of the multicellular hairs of its stems and leaves, i.e., they are elongate, eglandular, ferrugineous, and provided with numerous short, ascending side branches.
Observations of Tripsacum dactyloides and T. floridanum (Poaceae): measuring climate and soil gradient effects on Tripsacum in Texas, U.S.A.
Authors: McBryde Gary 
Published: November 2021

Reports of Tripsacum floridanum Porter ex Vasey in Texas have been countered with two arguments. First is that narrow-leafed Texas plants, when grown under favorable conditions, are more typical of wider-leafed T. dactyloides (L.) L. Second is that the offspring from crosses of T. dactyloides with T. floridanum are sterile or partially so. The support for these two claims is examined. The conclusion is narrow-leafed Texas plants typical of T. floridanum are in Texas, and crosses between the two produce fully fertile offspring. This raises the question of what factors are at play to segregate the wider-leafed from the narrower-leafed Texas populations. To investigate, two statistical models were constructed using separate data. First, a simultaneous system of seven equations was used to predict phenological and morphological plant characteristics given environmental factors. Second, a single logit equation predicted the probability of narrow-leafed diploid versus widerleafed diploid and tetraploid plants given environmental factors. Predictions allow for the measurement of plant characteristics from climate and soil gradients. Results suggest that narrow-leafed diploid plants occupy distinct habitats with the larger tetraploid plants coping by adapting mechanisms to overcome interspecies competition while the smaller diploid plants adapt with mechanisms to overcome stress. In conclusion, findings support consideration of T. floridanum as a variety of T. dactyloides and conservation implications are reviewed.
MORPHO-PHYSIOLOGICAL RESPONSES OF BOS INDICUS, BOS TAURUS AND CROSSBRED WEANED HEIFERS TO SEASONAL VARIATIONS
Authors: Pegoraro Mastelaro Ariadne , Sayuri Miyagi Eliane , Karvatte Junior Nivaldo , Romeiro de Oliveira Menezes Gilberto , Gustavo Macedo de Almeida Martins Paulo , Villa Alves Fabiana 
Published: November 2021

Animals kept in extensive production systems are constantly exposed to the effects of climatic aspects, which could favour thermal discomfort, decreasing productive and reproductive performances. Utilisation of Bos taurus crosses with Bos indicus animals is increasing in the tropics aimed at improving production and it is now well known how these animals respond in such environment. The objective was to characterize the morpho-physiological thermoregulation responses of weaned heifers from four different genetic groups to infer regarding the different degrees of adaptation to heat under tropical conditions Forty-eight, pasture-kept, 6-month-old, weaned heifers from four distinct genetic groups: (1) Nellore; (2) Senepol; (3) Angus x Nellore; and, (4) ¼ Brahman x ¼ Nellore x ½ Senepol (Tri-cross) were evaluated. Skin, hair coat and rectal temperatures; sweating rate; respiratory and heart rates; visual analysis of hair and hair coat colour; hair coat temperature and thickness; and length, diameter, density and number of hairs were evaluated. In addition, the temperature-humidity index, black-globe temperature and humidity index, and radiant thermal load were determined. Angus x Nellore animals presented the lowest (P=0.008) respiratory rate. Rectal temperature did differ (P=0.001) between seasons. Angus x Nellore heifers showed the greatest (P=0.001) values for the morphological measures in the evaluated months, coinciding with thermal comfort indexes above those considered comfortable for cattle. Nellore (Bos indicus), Brahman x Nellore x Senepol, and Senepol (B. taurus) present the best adaptive traits in savanna. The utilisation of B. indicus animals or adapted breed confers desirable morphological characteristics for the tropical environment.
Review Potensi Daun Awar-awar Sebagai Agen Ko-Kemoterapi Antimetastasis Kanker Payudara
Authors: Salsabila Dhiya Ulhaq , Faqiha Syifa Athia Zainun , Anggraeni Afivah Dewi , Ikawati Muthi' 
Published: November 2021

<p>Kematian akibat kanker payudara sebagian besar disebabkan oleh metastasis. Kemoterapi sebagai agen terapi kanker payudara juga menimbulkan efek samping metastasis. Daun awar-awar (<em>Ficus septica</em> Burm. F.) mengandung senyawa golongan alkaloid fenantroindolisidin. Ekstrak daun awar-awar dan alkaloid fenantroindolisidinnya berefek sitotoksik pada sel kanker payudara dan sinergis terhadap agen kemoterapi. Artikel ini mengulas potensi daun awar-awar sebagai agen pendamping kemoterapi (ko-kemoterapi), khususnya melalui mekanisme antimetastasis. Aktivitas sitotoksik daun awar-awar pada sel kanker terjadi melalui peningkatan apoptosis dan modulasi siklus sel. Daun awar-awar menghambat metastasis sel kanker payudara dengan menurunkan ekspresi VEGF, MMP-9, dan Rac-1 yang terlibat dalam metastasis. Oleh karena itu, dapat disimpulkan bahwa daun awar-awar berpotensi untuk dikembangkan sebagai agen ko-kemoterapi antimetastasis untuk kanker payudara. </p>
Analisis Hubungan Pengetahuan Terhadap Kepatuhan Terapi Pada Pasien Hipertensi di Puskesmas Pucang Sawit Surakarta
Authors: Farida Yeni , Salsabila Yumna Zulfa , Amsari Alfiani , Niruri Rasmaya , Yugatama Adi , Handayani Nestri , Prihapsara Fea 
Published: November 2021

<p>Hipertensi yang tidak terkendali dengan baik dapat meningkatkan terjadinya komplikasi, untuk itu diperlukan kepatuhan pasien dalam terapi hipertensi. Kepatuhan dipengaruhi oleh beberapa faktor, salah satu faktornya adalah pengetahuan. Tujuan penelitian adalah untuk mengetahui hubungan pengetahuan terhadap kepatuhan terapi pasien hipertensi di Puskesmas Pucangsawit Surakarta. Studi <em>crosssectional</em> dilakukan pada populasi pasien hipertensi di Puskesmas Pucang Sawit Surakarta yang memenuhi kriteria inklusi. Sampel ditetapkan dengan kriteria pasien mendapatkan terapi hipertensi minimal 1 bulan, melakukan kunjungan kontrol ke Puskesmas di bulan Maret 2019, serta bersedia menjadi responden yang dibuktikan dengan <em>informed consent</em>. Pengetahuan dinilai dengan kuesioner yang disusun peneliti berdasarkan panduan <em>pharmaceutical care </em>untuk penyakit hipertensi, sedangkan kepatuhan dinilai dengan kuesioner <em>hill-bone</em>. Pengetahuan dikategorikan menjadi baik, cukup dan kurang, sedangkan kepatuhan dikategorikan menjadi patuh dan tidak patuh. Analisis variabel dilakukan dengan uji <em>Pearson correlation</em>. Hasil penelitian menunjukkan bahwa 5,3% responden memiliki pengetahuan yang baik, 27,1% berpengetahuan cukup dan 17,6% berpengetahuan kurang. Tidak terdapat hubungan yang signifikan antara pengetahuan dengan kepatuhan terapi hipertensi pada pasien di Puskesmas Pucang Sawit Surakarta, sedangkan arah korelasinya positif tetapi korelasi antara keduanya sangat lemah (p = 0,319; r = 0,109). </p>
Membrane-Inducing “Masquelet” Technique for Massive Traumatic Diaphyseal Femur Bone Loss in a Pediatric Patient
Authors: Cardin Stefano , Garrett Christopher H. , Williams Warren A. , Langford Joshua R. , Herrera-Soto José A. 
Published: November 2021

Case:
A 14-year-old boy sustained 22 cm of femur bone loss after a motor vehicle accident. The patient underwent treatment with the membrane-inducing “Masquelet” technique for management of the injury. The grafts incorporated to form new bone and fill-in the void.

Conclusion:
Although the Masquelet technique is thoroughly described in adult orthopaedic trauma and oncology literature, there are minimal reports to support its use in pediatric patients. Five-year follow-up data on this patient concluded that utilization of this technique for significant bone defects proves to be a safe and effective alternative for the management of pediatric trauma patients.
Tuberculosis of the Cuboid in a 16-Month-Old Child
Authors: Patwardhan Sandeep , Sodhai Vivek , Sable Aashiket , Shyam Ashok , Sancheti Parag 
Published: November 2021

Case:
A 16-month-old male child presented with swelling over the dorsolateral aspect of the right foot associated with limp and no other constitutional symptoms. Tenderness and swelling were noted over the dorsolateral aspect, and radiographs revealed an eccentric lytic expansile lesion in the right cuboid. Biopsy of the lesion revealed necrotizing granulomas, and molecular testing later documented tuberculosis. The child received antitubercular medications and was relieved of symptoms in 6 weeks. Complete healing was observed after 12 months.

Conclusion:
In developing countries, a high index of suspicion helps in the early diagnosis and appropriate management of tubercular osteomyelitis in children.
An Unusual Presentation of Osteogenesis Imperfecta
Authors: Lindsay Sarah E. , Nicol Lindsey E. , Gamayo Ashley C. , Raney Ellen M. 
Published: November 2021

Case:
We report an 18-year-old patient with a clinical phenotype consistent with severe osteogenesis imperfecta (OI) with frequent fractures, short stature, shortening and bowing of extremities, and unusual radiographic features of severe fibrous dysplasia, including lytic lesions and a “ground-glass” appearance. Genetic testing for the patient was notable for a c.119C>T (p.Ser40Leu) variant in exon 1 of IFITM5 and a c.676C>A (Pro226Thr) variant in exon 5 of CREB3L1.

Conclusion:
This unusual skeletal presentation was in the setting of a rare IFITM5 mutation and represents a unique case of severe OI.
Bioorthogonal labeling of live dissociated and organotypic slice culture neurons
Authors: Bessa-Neto Diogo , Pecoraro Valeria , Beliu Gerti , Choquet Daniel 
Published: November 2021

Abstract
Over the past couple decades, the explosion in the development of high-resolution and super-resolution microscopy techniques has led to the need for the development of new protein labeling techniques. Click-labeling via genetic code expansion (GCE) has received particular attention given its potential has the ultimately small labelling probe for proteins. Click-labeling via GCE offers a reliable and sterically minimally demanding capacity to label proteins, but its application in non dividing cells such as neurons remains poorly exploited due to its low efficiency. Here, we describe a simple, efficient and reproducible protocol that allows to fluorescently label transmembrane proteins in live neurons using click-labeling via GCE, both in dissociated culture and organotypic brain slices.
Habitat formed by the invasive macroalga Caulerpa filiformis (Suhr) Hering (Caulerpales, Chlorophyta) alters benthic macroinvertebrate assemblages in Peru
Authors: Aguilar Silvia , Moore Pippa J. , Uribe Roberto A. 
Published: November 2021

Abstract
The green macroalga Caulerpa filiformis has been spreading on shallow soft sediment habitats along the Peruvian coast, colonizing previously unvegetated sediments to create monospecific meadows. We examined the nature of the impact of C. filiformis meadows on the density, taxonomic richness and assemblage structure of epifaunal and infaunal benthic macroinvertebrates. Specifically, we tested whether the spread of C. filiformis has resulted in different macroinvertebrate assemblages than those formed by the dominant native macroalgae (i.e., Rhodymenia spp.) and unvegetated sediments. Surveys were undertaken in two bays in each of two locations, in central and southern Peru, during winter 2017 and summer 2018. In general, our results show that macroinvertebrate assemblages were similar across all three habitats, although there were some differences, related to location and season, but with no clear patterns observed. Taxonomic richness and density was generally higher in the vegetated habitats than the unvegetated habitat, and where there were differences between the two vegetated habitats there was no consistent pattern of which habitat supported the highest richness or density. Given invading C. filiformis is primarily colonizing unvegetated habitats it would appear that this species is creating a new niche which supports similar assemblages, but higher taxonomic richness and density than unvegetated habitats. While our study suggests that C. filiformis is having a limited ecological impact we recommend that actions be put in place to limit the spread of this invasive species at the same time as increasing monitoring of the ecological impacts of this species as lags in the ecological impacts of invasive species are common.
Unnecessary Chest Tube for a Hemodialytic Patient with Multiple Trauma: A Case Report
Authors: Reisi-Vanani Vahid , Esfahani Hooman 
Published: November 2021

Abstract
Background

Pneumothorax (PTX) is a life-threatening condition that overdiagnosis could result in increases in mortality and morbidity of patients, this overdiagnosis would be increased if physicians do not manage the patient classically and do not pay attention to the physical exam and history of the patient.
Case presentation:

A-71-year old man was admitted to the emergency department due to multiple trauma. His vital signs were stable and in examination, there were two lacerations on his scalp with venous bleeding source and galea transaction; there were also some abrasions all over his body including his thorax. In the physical exam, there was no sucking lesion, decreases in respiratory sounds in auscultation or chest deformity but he had little right hemithorax rib tenderness. In more evaluations, there was a suspected visceral line of pleura in his CXR and no plural sliding movement was seen in E-FAST by the operator. Due to the inconsistency in physical exam and radiologic findings we decided to take a chest CT-scan before the insertion of the chest tube that indicated no PTX for him and the suspected visceral line in CXR was skin fold of a permacath for hemodialysis.
Conclusions

Several conditions could mimic findings of PTX in CXR that every physician should know and pay attention to them besides special attention to the history taking and physical examination to reduce the mortality and morbidity of patients.
Recombinantly Expressed MeICT, a new Toxin from Mesobuthus Eupeus Scorpion, Inhibits Glioma Cell Proliferation and Downregulates Annexin A2 and FOXM1 Genes
Authors: Ayat Hoda , Gandomkari Maryam Shahbazi , Ahadi Ali Mohammad 
Published: November 2021

Abstract
Gliomas are highly invasive and lethal malignancy that do not respond to current therapeutic approaches. Novel therapeutic agents are required to target molecular mechanisms involved in glioma progression. MeICT is a new short-chain toxin isolated from Mesobuthus eupeus scorpion venom. This toxin contained 34 amino acid residues and belongs to chloride channels toxins. In this study, the coding sequence of MeICT was cloned into the pET32Rh vector and a high yield of soluble recombinant MeICT was expressed and purified. Recombinant MeICT-His significantly inhibited the proliferation and migration of glioma cells at low concentration. In vivo studies showed that MeICT was not toxic when administrated to mice at high doses. We also determined the effect of MeICT on the mRNA expression of MMP-2, Annexin A2 and FOXM-2 that are key molecules in the progression and invasion of glioma. Expression of Annexin A2 and FOXM1 mRNA was significantly down-regulated following treatment with MeICT. However, no significant decrease in the expression of MMP-2 gene was identified. In this study a short toxin with four disulfide bonds was successfully produced and its anti-cancer effects was detected. Our findings suggest that recombinant MeICT can be considered as a new potent agent for glioma targeting.
Immunoglobulin (IgG4) Sclerosing Cholecystitis- Camouflaging Gall Bladder Cancer- A Case Report and Review of Literature
Authors: Singh Rashpal , Mahajan Puneet , Prasher Rizul , Rajdev Vivek , Singh Jagwinder , Kattoor Cliffin Mathai , Mardi Kavita , Gupta Jagdish 
Published: November 2021

Abstract
Background: IgG4 related disease is a rare systemic disorder having an underlying autoimmune cause. These disorders mainly affects pancreatico biliary tree i.e. pancreas, gall bladder and biliary tree (extrahepatic as well intrahepatic),but can also affect other part of body.Majority of disorders involving biliary tree are associated with autoimmune pancreatitis component.These disorders are difficult to diagnose clinically as they can mimic inflammatory as well malignancy and poses a real diagnostic challenge to manage and treat.Case presentation: 64 years old female known diabetic evaluated for pain in right hypochondrium. Gall bladder cancer was suspected clinically as well on radiologically basis. Patient underwent extended cholecystectomy as it was a resectable disease.Final histopathology revealed immunoglobulin G4 (IgG4) related cholecystitis which was confirmed after immunohistochemistry for CD 138 and IgG4.This disease could be managed conservatively by giving oral steroids ,if it has been picked up preoperatively and major surgical intervention have been avoided. No defined blood test or tumour markers are currently available to diagnose this entity except serum immunoglobulin G4 which is costly and not feasible to get done in all patients especially in developing nations like India.Conclusion: IgG4 cholecystitis is an immune mediated disease whose pathophysiology is still not completely understood. Every clinician should keep possibility of IgG4 cholecystitis in mind whenever any patient with abnormal gall bladder thickening or gall bladder mass is encountered in their clinical practice, as both these entities have completely different options of treatment. We should not rely solely upon clinical and radiological picture.
Effects of MRI on Stemness Properties of Wharton’s Jelly Derived Mesenchymal Stem Cells
Authors: Tashakori Mahnaz , Asadi Fatemeh , Khorram Faezeh-Sadat , Manshoori Azita , Hosseini-Chegeni Ali , Kamalabadi Mahdieh Ahmadi , Yousefi-Ahmadipour Aliakbar 
Published: November 2021

Abstract
BackgroundMesenchymal stem cells (MSCs), derived from various tissues, have served as a promising source of cells in clinic and regenerative medicine. Umbilical cord-Wharton’s jelly (WJ-MSCs)-derived MSCs exhibit advantages over those from adult tissues, such as no ethical concerns, shorter population doubling time, broad differentiation potential, readily available non-invasive source, prolonged maintenance of stemness properties. Material and methodsThe aim of this study was to evaluate the effect of MRI (1.5 T, 10 min) on stemness gene expression patterns (OCT-4, SOX-2, NANOG) of WJ-MSCs. In addition, we assessed cell viability, growth kinetics and apoptosis of WJ-MSCs after MRI treatment. ResultsThe quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR) data showed that transcript levels of SOX-2, NANOG in MRI-treated WJ-MSCs were increased 32- and 213-fold, respectively. MTT assay was performed at 24, 48, and 72 hours post-treatment and the viability was not significantly difference between two groups. The doubling time of MRI group was markedly higher than control group. In addition, the colony formation ability of WJ-MSCs after MRI treatment significantly increased. Furthermore, no change in apoptosis was seen before or after MRI treatment. ConclusionsOur results suggest the use of MRI can improve quality of MSCs and may enhance the efficacy of mesenchymal stem cell-based therapies.
Peri-Operative Medication Errors in a Tertiary Care Teaching Hospital of a Low-Middle Income Country
Authors: Abbasi Shemila , Rashid Saima , Khan Fauzia Anis 
Published: November 2021

Abstract
BackgroundIdentifying medication errors is one method of improving patient safety. Peri operative anesthetic management of patient includes polypharmacy and various steps prior to drug administration. Our objective was to analyze the medication errors reported in our critical incident reporting system (CIRS) database over the last 15 years (2004-2018) and to review measures taken for improvement based on the reported errors.MethodsAll Critical incidents (CI) reported during January 2004 till December 2018 were retrieved from CIRS database. Medication errors were identified and entered on a data extraction form which included reporting year, patients age, surgical specialty, ASA status, time of incident, phase and type of anesthesia and drug handling, type of error, class of medicine, level of harm, severity of adverse drug event (ADE) and steps taken for improvement.Results 311 medication errors were reported. Fifty two percent errors occurred in ASA II and III patient, and 43% during induction. Sixty % occurred during administration phase and 65 % were due to human error. Thirty seven percent were ADE, 58 of which were significant, 23 serious and five life-threatening errors. Majority errors involved neuromuscular blockers (32%) and opioids (13%).Conclusion Sharing of CI and a lesson to be learnt e-mail, colour coded labels, change in medication trolley lay out, decrease in floor stock and high alert labels were the low-cost steps taken to reduce incidents.Medication errors were more frequent during administration. Twenty eight percent resulted in significant, serious, or life-threatening events.