bluuebunny/crossref_metadata_2025_split huggingface dataset:
Assessment of Bioanalytical Method Validation Data Utilizing Heteroscedastic Seven-Point Linear Calibration Curves by EZSTATSG1 Customized Microsoft Excel Template
Authors: Sofalvi Szabolcs , Schueler Harold E 
Published: May 2021

Abstract
Bioanalytical methods developed in accredited forensic toxicology laboratories need to be validated according to the ISO/IEC 17025 standards to demonstrate that a method is fit for the intended use. The evaluation of the validation data can be a time-consuming process without a streamlined procedure on a common platform or the aid of a specific software package. The objective of this work was to develop a Microsoft (MS) Excel (Redmond, WA, USA) template, which can rapidly generate all the required numerical and statistical validation results of a quantitative bioanalytical assay with minimal data entry and to provide documented traceability of the validation data directly to the instrument raw data. Evaluation of method validation data can be done easily by taking advantage of the power of statistical tools available within Excel. The three key features of the template were the development of six pertinent weighted linear calibration models, the variance in instrument response as a function of concentration and integrated one-way analysis-of-variance (ANOVA) tables. None of the ANOVA tables in this template need to be recreated whenever the source data changes, which is a major time savings when a method contains several dozen analytes. It takes ∼60 minutes per analyte to enter all the instrument raw data manually from the chromatograms. The final method validation results are summarized in a two-page validation summary report, whereas the associated data tables and graphs are presented in an additional 65 pages of digital information, which can be saved for electronic records using portable document format (PDF). This EZSTATSG1.xltm customized MS Excel method validation template and a completed PDF example are being provided as downloadable files in the Supplementary data link to offer an option to the scientific community for expediting the evaluation of validation data.
pH-regulated chaperone function of cyanobacterial Hsp90 and Hsp70: implications for light/dark regulation
Authors: Akter Tahmina , Nakamoto Hitoshi 
Published: May 2021

Abstract
We have shown that cyanobacterial chaperonins have pH-dependent anti-aggregation activity. The pH in cyanobacterial cytosol increases by one pH unit following a shift from darkness to light. In this study, we examined whether other major chaperones such as Hsp90 (HtpG) and Hsp70 (DnaK2) from the cyanobacterium Synechococcus elongatus PCC 7942 also display pH-dependent activity. Suppressing aggregation of various heat-denatured proteins, especially lactate dehydrogenase, at an equimolar ratio of cyanobacterial Hsp90 to protein substrate was found to be pH-dependent. Hsp90 showed the highest activity at pH 8.5 over the examined pH range of 7.0 to 8.5. pH affected the anti-aggregation activity of DnaK2 in a similar manner to that of Hsp90 in the presence of half equimolar DnaK2 to the protein substrate. The ATPase activity of cyanobacterial Hsp90 was pH-dependent, with a fourfold increase in activity when the pH was raised from 7.0 to 8.5. The ATPase activity of DnaK2 was also regulated by pH in a similar manner. Finally, an increase in pH from 7.0 to 8.5 enhanced activities of both Hsp90 and Hsp70 in protein-folding assistance by two- to threefold. These results suggest that changes in pH may regulate chaperone function during a light–dark cycle in cyanobacterial cells.
Disjunction in Negative Contexts: A Cross-Linguistic Experimental Study
Authors: Lungu Oana , Fălăuș Anamaria , Panzeri Francesca 
Published: May 2021

Abstract
This squib reports experimental findings from a study investigating the interpretation of simple disjunction in negative contexts in four languages: Italian, French, English, Romanian. We provide evidence that casts doubt on the robustness of the distinction between PPI disjunction languages and non-PPI disjunction languages. The difference turns out to be less clear-cut than assumed in the theoretical (e.g., Szabolcsi 2002, Spector 2014, Nicolae 2017) or experimental literature (e.g., Crain 2012, Guasti et al. 2017). The results reported here inform current accounts of positive polarity and flesh out some methodological issues raised by the various tasks used in experimental investigations of the polarity sensitivity of disjunction.
The use of Indocyanine green fluorescent in patients with abdominal trauma for better intraoperative decision-making and less bowel anastomosis leak: case series
Authors: Afifi Ibrahim , Abdelrahman Husham , El-Faramawy Ahmed , Mahmood Ismail , Khoschnau Sherwan , Al-Naimi Noof , El-Menyar Ayman , Al-Thani Hassan , Rizoli Sandro 
Published: May 2021

Abstract
Despite technological advances in the management of blunt abdominal trauma, the rate of bowel anastomotic leakage (AL) remains high. The etiology of AL is multifactorial, but insufficient blood perfusion is considered to play a substantial role in the pathogenesis. In recent years, angiography with Indocyanine green (ICG), a fluorescent dye, has been introduced in the clinical practice to assess organ perfusion in several conditions. Given the scarcity of publications describing the use of ICG in trauma patients as a potentially useful strategy that may facilitate intraoperative decisions and limit the extent of bowel resection, we presented the utility of intraoperative ICG fluorescent in abdominal trauma patients in a level 1 trauma center. The use of ICG fluoroscopy in patients with abdominal trauma is feasible and useful; however, large prospective studies in trauma patients are warranted.
Primary omental torsion diagnosed and treated laparoscopically: a case report
Authors: Imanishi Kentaro , Iga Norichika , Mizuno Daisuke , Nishi Hideyuki , Miyoshi Shinichiro 
Published: May 2021

Abstract
Omental torsion, a rare cause of acute abdomen in children and adults, is difficult to correctly diagnose before surgery because it mimics the common causes of acute surgical abdomen. We present a case of greater omental torsion that was diagnosed by laparoscopy. A 37-year-old man presented with right lower abdominal pain and was suspected to have appendicitis. Blood tests revealed elevated C-reactive protein and white blood cell count, whereas computed tomography revealed a nodular mass and high-density lesions in the fat tissue. As the patient’s abdominal symptoms were severe and a clear diagnosis could not be made, we performed exploratory laparoscopy. Laparoscopy revealed omental torsion, and an omentectomy was performed. The patient’s pain had significantly reduced post-surgery, and post-operative recovery was uneventful. Thus, laparoscopic examination is useful for accurately diagnosing omental torsion and is less invasive than surgery.
A tough 3D puzzle in the walnut shell
Authors: Elbaum Rivka , Elbaum Michael 
Published: May 2021

This article comments on:
Antreich SJ, Xiao N, Huss JC, Gierlinger N. 2021. A belt for the cell: cellulosic wall thickenings and their role in morphogenesis of the 3D puzzle cells in walnut shells. Journal of Experimental Botany 72,4744–4756.
Magnetic angle evolution in accreting neutron stars
Authors: Biryukov Anton , Abolmasov Pavel 
Published: May 2021

ABSTRACT
The rotation of a magnetized accreting neutron star (NS) in a binary system is described by its spin period and two angles: spin inclination α with respect to the orbital momentum and magnetic angle χ between the spin and the magnetic moment. Magnetospheric accretion spins the NS up and adjusts its rotation axis, decreasing α to nearly perfect alignment. Its effect upon the magnetic angle is more subtle and relatively unstudied. In this work, we model the magnetic angle evolution of a rigid spherical accreting NS. We find that the torque spinning the NS up may affect the magnetic angle while both α and χ significantly deviate from zero, and the spin-up torque varies with the phase of the spin period. As the rotation axis of the NS is being aligned with the spin-up torque, the magnetic axis becomes misaligned with the rotation axis. Under favourable conditions, magnetic angle may increase by Δχ ∼ 15°−20°. This orthogonalization may be an important factor in the evolution of millisecond pulsars, as it partially compensates the χ decrease potentially caused by pulsar torques. If the direction of the spin-up torque changes randomly with time, as in wind-fed high-mass X-ray binaries, both the rotation axis of the NS and its magnetic axis become involved in a non-linear random-walk evolution. The ultimate attractor of this process is a bimodal distribution in χ peaking at χ = 0° and χ = 90°.
Mass calibration of distant SPT galaxy clusters through expanded weak-lensing follow-up observations with HST, VLT, & Gemini-South
Authors: Schrabback T , Bocquet S , Sommer M , Zohren H , van den Busch J L , Hernández-Martín B , Hoekstra H , Raihan S F , Schirmer M , Applegate D , Bayliss M , Benson B A , Bleem L E , Dietrich J P , Floyd B , Hilbert S , Hlavacek-Larrondo J , McDonald M , Saro A , Stark A A , Weissgerber N 
Published: May 2021

ABSTRACT
Expanding from previous work, we present weak-lensing (WL) measurements for a total sample of 30 distant (zmedian = 0.93) massive galaxy clusters from the South Pole Telescope Sunyaev–Zel’dovich (SPT-SZ) Survey, measuring galaxy shapes in Hubble Space Telescope (HST) Advanced Camera for Surveys images. We remove cluster members and preferentially select z ≳ 1.4 background galaxies via V − I colour, employing deep photometry from VLT/FORS2 and Gemini-South/GMOS. We apply revised calibrations for the WL shape measurements and the source redshift distribution to estimate the cluster masses. In combination with earlier Magellan/Megacam results for lower-redshifts clusters, we infer refined constraints on the scaling relation between the SZ detection significance and the cluster mass, in particular regarding its redshift evolution. The mass scale inferred from the WL data is lower by a factor $0.76^{+0.10}_{-0.14}$ (at our pivot redshift z = 0.6) compared to what would be needed to reconcile a flat Planck νΛCDM cosmology (in which the sum of the neutrino masses is a free parameter) with the observed SPT-SZ cluster counts. In order to sensitively test the level of (dis-)agreement between SPT clusters and Planck, further expanded WL follow-up samples are needed.
Spectroscopic signatures of the vanishing natural coronagraph of Eta Carinae
Authors: Damineli A , Navarete F , Hillier D J , Moffat A F J , Corcoran M F , Gull T R , Richardson N D , Weigelt G , Morris P W , Stevens I 
Published: May 2021

ABSTRACT
Eta Carinae is a massive interacting binary system shrouded in a complex circumstellar environment whose evolution is the source of the long-term brightening observed during the last 80 yr. An occulter, acting as a natural coronagraph, impacts observations from our perspective, but not from most other directions. Other sight-lines are visible to us through studies of the Homunculus reflection nebula. The coronagraph appears to be vanishing, decreasing the extinction towards the central star, and causing the star’s secular brightening. In contrast, the Homunculus remains at an almost constant brightness. The coronagraph primarily suppresses the stellar continuum, to a lesser extent the wind lines, and not the circumstellar emission lines. This explains why the absolute values of equivalent widths (EWs) of the emission lines in our direct view are larger than those seen in reflected by the Homunculus, why the direct view absolute EWs are decreasing with time, and why lower-excitation spectral wind lines formed at larger radii (e.g Fe ii 4585 Å) decrease in intensity at a faster pace than higher excitation lines that form closer to the star (e.g. H δ). Our main result is that the star, despite its 10-fold brightening over two decades, is relatively stable. A vanishing coronagraph that can explain both the large flux evolution and the much weaker spectral evolution. This is contrary to suggestions that the long-term variability is intrinsic to the primary star that is still recovering from the Great Eruption with a decreasing mass-loss rate and a polar wind that is evolving at a slower pace than at the equator.
Identity and compatibility of reference genome resources
Authors: Stolarczyk Michał , Xue Bingjie , Sheffield Nathan C 
Published: May 2021

Abstract
Genome analysis relies on reference data like sequences, feature annotations, and aligner indexes. These data can be found in many versions from many sources, making it challenging to identify and assess compatibility among them. For example, how can you determine which indexes are derived from identical raw sequence files, or which annotations share a compatible coordinate system? Here, we describe a novel approach to establish identity and compatibility of reference genome resources. We approach this with three advances: first, we derive unique identifiers for each resource; second, we record parent–child relationships among resources; and third, we describe recursive identifiers that determine identity as well as compatibility of coordinate systems and sequence names. These advances facilitate portability, reproducibility, and re-use of genome reference data. Available athttps://refgenie.databio.org.
Haemodialysis patients show a highly diminished antibody response after COVID-19 mRNA vaccination compared with healthy controls
Authors: Simon Benedikt , Rubey Harald , Treipl Andreas , Gromann Martin , Hemedi Boris , Zehetmayer Sonja , Kirsch Bernhard 
Published: May 2021

Abstract

Background
Haemodialysis (HD) patients are exposed to a high risk due to the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic. They are prone to acquiring the infection and are threatened by high mortality rates in case of infection. However, HD patients were not included in the efficacy trials of the SARS-CoV-2 vaccines. Such efficacy data would have been critical because HD patients show decreased responses against various other vaccines and this could translate to the SARS-CoV-2 vaccines as well.

Methods
We conducted a prospective cohort study that contained a group of 81 HD patients and 80 healthy controls. All of them had been vaccinated with the BioNTech/Pfizer mRNA vaccine (two doses, as per the manufacturer’s recommendation). The anti-SARS-CoV-2 spike (S) antibody response was measured for all participants 21 days after the second dose. The groups were compared using univariate quantile regressions and a multivariate analysis. The adverse events (AEs) of the vaccination were assessed via a questionnaire. Finally, a correlation between the HBs-antibody response and the SARS-CoV-2 antibody response in the HD patients was established.

Results
The HD patients had significantly lower anti-SARS-CoV-2 S antibody titres than the control patients 21 days after vaccination (median was 171 U/mL for dialysis patients and 2500 U/mL for the controls). Further, the HD group presented fewer AEs than the control group. No correlation was found between the antibody response to previous Hepatitis B vaccination and that of the SARS-CoV-2 vaccine.

Conclusions
HD patients present highly diminished SARS-CoV-2 S antibody titres compared with a cohort of controls. Therefore, they could be much less protected by SARS-CoV-2 mRNA vaccinations than expected. Further studies to test alternative vaccination schemes should be considered.
Extended Retrosigmoid Craniotomy and Approach Through the Glossopharyngeal Cochlear Triangle for Clipping of a High-Riding Vertebral-Posterior Inferior Cerebellar Artery Aneurysm: 2-Dimensional Operative Video
Authors: Peitz Geoffrey W , McDermott Ryan A , Baranoski Jacob F , Lawton Michael T , Mascitelli Justin R 
Published: May 2021

Abstract
The far lateral transcondylar (FL) craniotomy is the standard approach for posterior inferior cerebellar artery (PICA) aneurysm exposure through microsurgical dissection in the vagoaccessory triangle (VAT).
1,2
However, the extended retrosigmoid (eRS) craniotomy and dissection through the glossopharyngeal-cochlear triangle (GCT) may be more appropriate when the patient has an aneurysm arising from a high-riding vertebral artery (VA)—PICA origin.
3-5
We present a case of a 41-yr-old woman with hypertension presenting with left occipital pain and left-side hearing loss and past facial spasm and pain. Computed tomography angiography and digital subtraction angiography demonstrated an unruptured 8.4 × 9.0 × 10.2 mm saccular aneurysm at the left VA-PICA junction. Surgical clipping was chosen over endovascular therapy given the relationship of the PICA origin to the aneurysm neck as well as the history of cranial neuropathy. It was noted that the VA-PICA junction and aneurysm was high-riding at the level of the internal auditory canal. An eRS craniotomy was performed with dissection through the GCT, and the aneurysm was clipped as shown in the accompanying 2-dimensional operative video. Postoperative angiography demonstrated complete occlusion of the aneurysm and patency of the left VA and PICA without stenosis, and the patient had a favorable postoperative course although her left-sided hearing remained diminished. The eRS craniotomy allowed direct exposure via the GCT for clipping of the high-riding VA-PICA junction aneurysm and decompression of the cranial nerves. The traditional FL craniotomy and exposure through the VAT would likely have resulted in a less desirable inferior trajectory.
The patient gave informed consent for the operation depicted in the video.
Animation at 2:43 in video is used with permission from Barrow Neurological Institute, Phoenix, Arizona.
Sirach
Authors: Corley Jeremy 
Published: May 2021

Abstract
This survey article on the Book of Sirach (Latin: Ecclesiasticus) first considers its composition by a Jerusalem scribe named Ben Sira, who lived in the early second century bce. The article then examines major themes in the book’s theology (wisdom, creation, theodicy, and death), as well as its much-criticized portrayal of women. The chapter also explores its ethics of honor, generosity, and friendship, as well as the review of biblical history in the Praise of the Ancestors (Sirach 44–50). Thereafter, the chapter notes the book’s parallels to the New Testament, as well as its mixed reception history within Jewish, Catholic, and Protestant faith communities. Finally, to illustrate aspects of the reception history, the article offers four case studies showing how various later interpreters have used the sage’s teaching on testing (2:1–18), friendship (6:5–17), social justice (34:21–31), and remembering godly ancestors (44:1–15).
The Additions of the Greek Book(s) of Esther
Authors: Smith Tyler , de Troyer Kristin 
Published: May 2021

Abstract
One of the main issues with the Additions to the Greek version of Esther is that it needs to explain the relation between the Additions and the core text of the Esther story as present in the Old Greek or the Septuagint. For instance, with regard to Additions B and E, it has to be explained how the surrounding text after 3:13 and 8:13 was adapted to fit the contents of B and E, respectively. Similarly, with regard to Addition D, any explanatory theory needs to show how the text of 5:1–2 was rewritten in both the Old Greek (OG) and the Alpha Text (AT) to become what is now Addition C. Given the complexity of the relationship between the Masoretic Text, OG, and AT (and the daughter versions) and the similarities and the differences between the text of the Additions in the OG and the AT, an all-explaining theory remains a desideratum.
1 Esdras (Greek Ezra)
Authors: Grabbe Lester L. 
Published: May 2021

Abstract
1 Esdras is a Greek book parallel to Ezra and parts of Nehemiah and 2 Chronicles 35–36 in the Hebrew canon. There has been a long debate on the relationship of 1 Esdras to the Hebrew books. Three main theories are examined here, along with a summary of the book’s contents. The thesis favored in this essay is that 1 Esdras represents in some fashion an earlier stage of the Zerubbabel–Joshua and the Ezra traditions from which the compiler of the Hebrew Ezra–Nehemiah drew. 1 Esdras was not, however, the specific source used but is itself also a development of that source. The tradition picked up by the Hebrew Ezra–Nehemiah apparently did not have the story of the guards’ contest. This and other considerations indicate that this story was added at a later date to 1 Esdras.
Baruch
Authors: Hogan Karina Martin 
Published: May 2021

Abstract
Despite being cited relatively rarely by ancient exegetes, the book of Baruch enjoyed authority and inclusion in most Bible manuscripts because of its association with the prophet Jeremiah. In modern scholarship, the book of Baruch can be read as an example of the creative reuse of scriptures by a Jewish author (or authors) of the Hellenistic or Roman period. Its message about alienation, sin, repentance, and restoration is presented from different angles through the genres of confessional prayer, wisdom poem, and prophetic poem of consolation. Its Deuteronomic theology, which unites the various parts of the book, is very typical of the literature of ancient Judaism. At the same time, it is a powerful reminder to take responsibility for one’s own part in perpetuating structures of social injustice, even when one feels oppressed by them.
The US–Mexican War
Authors: Winders Bruce 
Published: May 2021

Abstract
Usually thought of as a two-year-long conflict between the United States and Mexico, the US–Mexican War (1846–1848) represents the culmination of a much longer struggle over the control of what became the American Southwest. Years before Mexico declared its independence, early citizens of the United States resolved to seize Spain’s North American possessions. Devastated by a decade of revolt, Mexico lacked the unity needed to halt American efforts to acquire land at its expense. The US–Mexican War revealed an important divide among Mexicans over the issue of federalism. In the end, the Treaty of Guadalupe Hidalgo defined the modern political boundary between the two nations. Far from bringing peace to either nation, though, the war generated internal strife for both the United States and Mexico. The historic conflict still affects the relationship between the two nations.
Romanticism in Mexico
Authors: Shelton Laura 
Published: May 2021

Abstract
During the nineteenth century, romanticism became central to how Mexicans engaged in practices of self-definition. Romanticism in Mexico was an intellectual and artistic movement that was at once autonomous and connected to transcultural influences. As evidenced by the works of historians and literary scholars, as well as novelists, politicians, poets, and antiquarians from the period, romanticism was gendered in terms of women’s participation and representation, and in themes such as love, family, virtue, domesticity, and eroticism. Women were critical to the transmission of romanticism in quotidian practices of attending theater and opera, hosting salons, and instilling appreciation for poetry and the natural world in their families and their communities. Romanticism also exercised a profound influence on how Mexicans thought about ethnicity, race, and nationalism. In their quest for a unique national identity, Mexican intellectuals looked to the indigenous past and celebrated mestizaje as the foundation of Mexico’s cultural patrimony, even as they persisted in exclusionary practices toward their indigenous and casta compatriots. Romanticism offers a fruitful area to reevaluate well-studied themes of Mexican history, particularly its complex relationship with nationalism, modernization, gender, and the politics of ethnicity and race.