bluuebunny/crossref_metadata_2025_split huggingface dataset:
The mechanism of artemisinin resistance of Plasmodium falciparum malaria parasites originates in their initial transcriptional response
Authors: Zhu Lei , van der Pluijm Rob W. , Kucharski Michal , Nayak Sourav , Tripathi Jaishree , Nosten François , Faiz Abul , Amaratunga Chanaki , Lek Dysoley , Ashley Elizabeth A , Smithuis Frank , Phyo Aung Pyae , Lin Khin , Imwong Mallika , Mayxay Mayfong , Dhorda Mehul , Chau Nguyen Hoang , Thuy Nhien Nguyen Thanh , Newton Paul N , Jittamala Podjanee , Tripura Rupam , Pukrittayakamee Sasithon , Peto Thomas J , Miotto Olivo , Seidlein Lorenz von , Hien Tran Tinh , Ginsburg Hagai , Day Nicholas PJ , White Nicholas J. , Dondorp Arjen M , Bozdech Zbynek 
Published: May 2021

AbstractThe emergence and spread of artemisinin resistant Plasmodium falciparum, first in the Greater Mekong Subregion (GMS), and now in East Africa, is a major threat to global malaria eliminations ambitions. To investigate the artemisinin resistance mechanism, transcriptome analysis was conducted of 577 P. falciparum isolates collected in the GMS between 2016-2018. A specific artemisinin resistance-associated transcriptional profile was identified that involves a broad but discrete set of biological functions related to proteotoxic stress, host cytoplasm remodeling and REDOX metabolism. The artemisinin resistance-associated transcriptional profile evolved from initial transcriptional responses of susceptible parasites to artemisinin. The genetic basis for this adapted response is likely to be complex.One sentence summaryThe transcriptional profile that characterize artemisinin resistant infections with malaria parasites Plasmodium falciparum originates in the initial transcriptional response to the drug.
Diel CO2 fluctuations alter the molecular response of coral reef fishes to ocean acidification conditions
Authors: Schunter Celia , Jarrold Michael D. , Munday Philip L. , Ravasi Timothy 
Published: May 2021

AbstractEnvironmental CO2 variation can modify the responses of marine organisms to ocean acidification, yet the underlying mechanisms for this effect remain unclear. On coral reefs, environmental CO2 fluctuates on a regular day-night cycle. Effects of future ocean acidification on coral reef fishes might therefore depend on their response to this diel cycle of CO2. To evaluate the effects on the brain molecular response, we exposed two common reef fishes (Acanthochromis polyacanthus and Amphiprion percula) to two projected future CO2 levels (750 and 1,000 μatm) under both stable and diel fluctuating conditions. We found a common signature to stable elevated pCO2 for both species, which included the downregulation of immediate early genes, indicating lower brain activity. The transcriptional program was more strongly affected by higher average CO2 in a stable treatment than for fluctuating treatments, however, the largest difference in molecular response was between stable and fluctuating CO2 treatments. This indicates that a response to a change in environmental CO2 conditions is different for organisms living in a fluctuating than in stable environments. The differential regulation was related to steroid hormones and circadian rhythm (CR). Both species exhibited a marked difference in the expression of CR genes among CO2 treatments, possibly accommodating a more flexible adaptive approach to acid-base control, which could explain reduced impairment. Our results suggest that environmental CO2 fluctuations might enable reef fishes to phase shift their clocks and anticipate CO2 changes, thereby avoiding impairments and more successfully adjust to ocean acidification conditions.
Genomic and physiological characterization of new Plant Growth Promoting Bacilli isolated from salt-pans
Authors: Petrillo Claudia , Castaldi Stefany , Lanzilli Mariamichela , Selci Matteo , Cordone Angelina , Giovannelli Donato , Isticato Rachele 
Published: May 2021

AbstractMassive application of chemical fertilizers and pesticides has been the main strategy used to cope with the rising crop demands in the last decades. The indiscriminate use of chemicals while providing a temporary solution to food demand has led to a decrease in crop productivity and an increase in the environmental impact of modern agriculture. A sustainable alternative to the use of chemicals for crop production is the use of microorganisms naturally capable of enhancing plant growth and protecting crops from pests, known as Plant-Growth-Promoting Bacteria (PGPB). Aim of the present study was to isolate and characterize PGPB from salt-pans sand samples able to ameliorate plant fitness. To survive high salinity, salt-tolerant microbes produce a broad range of compounds with heterogeneous biological activities that are potentially beneficial for plant growth. We have isolated and screenedin vitroa total of 20 halophilic spore-forming bacteria for phyto-beneficial traits and compared the results with twoBacillirecently isolated from the rhizosphere of the same collection site and recently characterized as potential biocontrol agents. Whole-genome analysis on five selected halophilic strains confirmed the presence of numerous gene clusters with PGP and biocontrol functions and of novel secondary-metabolite biosynthetic genes potentially involved in plant growth promotion and protection. The predicted biocontrol potential was confirmed in dual culture assays against several phytopathogenic fungi and bacteria. Interestingly, the absence of predicted gene clusters with known biocontrol functions in some of the isolates was not predictive of thein vitroresults, supporting the need of combining laboratory assays and genome mining in PGPB identification for future applications.
Detection of Campylobacter in air samples from poultry houses using shot-gun metagenomics – a pilot study
Authors: Haverkamp Thomas H.A. , Spilsberg Bjørn , Johannessen Gro S. , Torp Mona , Sekse Camilla 
Published: May 2021

AbstractBackgroundFoodborne pathogens such as Campylobacter jejuni are responsible for a large fraction of the gastrointestinal infections worldwide associated with poultry meat. Campylobacter spp. can be found in the chicken fecal microbiome and can contaminate poultry meat during the slaughter process. The current standard methods to detect these pathogens at poultry farms use fecal dropping or boot swaps in combination with cultivation / PCR. In this study, we have used air filters in combination with shotgun metagenomics for the detection of Campylobacter in poultry houses and MOCK communities to test the applicability of this approach for the detection of foodborne pathogens.ResultsThe spiked MOCK communities showed that we could detect as little as 200 CFU Campylobacter per sample using our protocols. Since we were interested in detecting Campylobacter, a DNA extraction protocol for Gram negative bacteria was chosen, and as expected, we found that the DNA extraction protocol created a substantial bias affecting the community composition of the MOCK communities. It can be expected that the same bias is present for poultry house samples analyzed. We observed significant amounts of Campylobacter on the air filters using both real-time PCR as well as shotgun metagenomics, irrespective of the amount of spiked in Campylobacter cells, suggesting that the flocks in both houses harboured Campylobacter spp.. Interestingly, in both houses we find diverse microbial communities present in the indoor air. In addition, have we tested the Campylobacter detection rate using shotgun metagenomics by spiking with different levels of C. jejuni cells in both the mock and the house samples. This showed that even with limited sequencing Campylobacter is detectable in samples with low abundance.ConclusionsThese results show that air sampling of poultry houses in combination with shotgun metagenomics can detect and identify Campylobacter spp. present at low levels. This is important since early detection of Campylobacter in food production can help to decrease the number of food-borne infections.
Gymnasts’ ability to modulate bioelectric sensorimotor rhythms during kinesthetic motor imagery of sports non-specific movements
Authors: Sugino Hirotaka , Ushiyama Junichi 
Published: May 2021

AbstractPrevious psychological studies using questionnaires have consistently reported that athletes have superior motor imagery ability, both for sports-specific and sports non-specific movements. However, regarding motor imagery of sports non-specific movements, no physiological studies have demonstrated differences in neural activity between athletes and non-athletes. The purpose of the present study was to examine differences in bioelectric sensorimotor rhythms during kinesthetic motor imagery (KMI) of sports non-specific movements between gymnasts and non-gymnasts. We selected gymnasts as an example population because they are likely to have particularly superior motor imagery ability due to frequent usage of motor imagery including KMI as part of daily practice. Healthy young participants (16 gymnasts and 16 non-gymnasts) performed repeated motor execution and KMI of sports non-specific movements (wrist dorsiflexion and shoulder abduction of the dominant hand). Scalp electroencephalogram (EEG) was recorded over the contralateral sensorimotor cortex. During motor execution and KMI, sensorimotor EEG power is known to decrease in the α- (8–15 Hz) and β-bands (16–35 Hz), referred to as event-related desynchronization (ERD). We calculated the maximal peak of ERD both in the α- (αERDmax) and β-bands (βERDmax) as a measure of changes in corticospinal excitability. αERDmax was significantly greater in gymnasts, who subjectively evaluated their KMI as being more vivid, for both KMI tasks. On the other hand, βERDmax was greater in gymnasts only for shoulder abduction KMI. These findings suggest gymnasts’ signature of flexibly modulating sensorimotor rhythm with no movements, which may be the basis of their superior ability of KMI for sports non-specific movements.New & NoteworthyKinesthetic motor imagery of sports non-specific movements was compared between gymnasts and non-gymnasts (i.e., healthy controls) from both physiological and psychological approaches. The EEG sensorimotor rhythms during kinesthetic motor imagery were more desynchronized in gymnasts who subjectively imaged their own movements as being more vivid. The work reveals novel ability in gymnasts to flexibly control their sensorimotor rhythms with no actual movements, which would be the basis of their superior ability of motor imagery.
Twenty-one Year Trends for Shorebirds, Waterfowl, and Other Waterbirds at Great Salt Lake, Utah
Authors: Tavernia Brian G. , Meehan Tim , Neill John , Luft John 
Published: May 2021

AbstractMillions of wetland-dependent birds annually depend on saline lakes and associated wetlands in the western United States. Understanding the population status and trends of birds with different life histories and habitats can guide efforts to secure water resources needed to sustain bird habitats. We used a 21-year dataset to examine population trends for 24 survey units presumed to be high-quality habitat for migratory shorebirds, waterfowl, and other waterbirds at Great Salt Lake and associated wetlands. As expected for high-quality habitats, we found stable or positive trends for 36 of 37 species or groups in fall, spring, or both seasons when considering survey units in aggregate. Despite stable or positive aggregate trends, negative trends did occur in some individual survey units. Foraging, migration distance, and taxonomic groupings were unrelated to trend direction. Research is needed to test whether survey units represent high-quality habitat. With declining regional water resources, stable and positive aggregate trends reinforce the importance of surveyed units at Great Salt Lake and associated wetlands to wetland-dependent birds. Ensuring continuation of stable and positive trends will require identifying environmental factors - including water quantity and quality - driving trends, and require coordinated regional management and monitoring of wetland-dependent birds.
The impact of depressive symptoms on response to integrated cognitive behavioral therapy for substance use disorders and intimate partner violence
Authors: Kelly Lourah M. , Crane Cory A. , Zajac Kristyn , Easton Caroline J. 
Published: May 2021

Purpose
Past studies demonstrated the efficacy of integrated cognitive-behavioral therapy (CBT) for substance use disorder (SUD) and intimate partner violence (IPV) as well as high rates of depressive symptoms in this population. However, little is known about how depressive symptoms impact treatment outcomes. The authors hypothesized that integrated CBT, but not standard drug counseling (DC), would buffer the negative effects of depressive symptoms on treatment response.

Design/methodology/approach
A secondary analysis of a randomized trial compared men assigned to 12 weeks of integrated CBT for SUD and IPV (n = 29) to those in DC (n = 34).

Findings
Most (60%) of the sample reported any depressive symptoms. Controlling for baseline IPV, reporting any depressive symptoms was associated with more positive cocaine screens during treatment. Among men with depressive symptoms, integrated CBT but not DC was associated with fewer positive cocaine screens. Controlling for baseline alcohol variables, integrated CBT and depressive symptoms were each associated with less aggression outside of intimate relationships (family, strangers, etc.) during treatment. For men without depressive symptoms, integrated CBT was associated with less non-IPV aggression compared to DC. Effects were not significant for other substances, IPV, or at follow-up.

Research limitations/implications
This study found some evidence for differential response to CBT by depressive symptoms on cocaine and aggression at end of treatment, which did not persist three months later. Future studies should explore mechanisms of integrated CBT for SUD and IPV, including mood regulation, on depressive symptoms in real-world samples.

Practical implications
Integrated CBT buffered depressive symptoms’ impact on cocaine use, yet only improved non-IPV aggression in men without depressive symptoms.

Originality/value
Although integrated CBT’s efficacy for improving SUD and IPV has been established, moderators of treatment response have not been investigated.
Age-inclusive HR practices and the thriving of older workers: the mediating role of occupational future time perspective
Authors: Oliveira Eduardo 
Published: May 2021

PurposeDrawing on social exchange theory and socio-emotional selectivity theory, this paper examines the role of occupational future time perspective (OFTP) in the relationship between age-inclusive HR practices (AIHRP) and the thriving of older workers.Design/methodology/approachA two-wave cross-sectional design was adopted with bootstrapped parallel multiple mediation analyses. In addition, polynomial regression with response surface analysis was used to examine the extent to which combinations of focus on opportunities and remaining time relate to thriving at work. Data were collected from 310 older workers working in 13 companies located in Portugal.FindingsAIHRP have direct effects on OFTP dimensions (i.e. focus on opportunities and remaining time), and indirect effects on the two thriving dimensions (i.e. learning and vitality) via focus on opportunities. The positive relationship between AIHRP and learning was mediated by remaining time, while no significant mediating effect on vitality through remaining time was found. Additionally, surface analysis showed that overall thriving and learning increase more sharply when focus on opportunities is higher than remaining time, rather than vice versa.Originality/valueThis paper contributes to the literature by showing the importance of personal resources like OFTP in the relationship between AIHRP and the thriving of older workers. It also provides further support for the distinctiveness of the two OFTP dimensions as remaining time was not linked to vitality, whereas focus on opportunities was linked to both thriving dimensions.
A new hyperchaotic system from T chaotic system: dynamical analysis, circuit implementation, control and synchronization
Authors: Emiroglu Selcuk , Akgül Akif , Adıyaman Yusuf , Gümüş Talha Enes , Uyaroglu Yılmaz , Yalçın Mehmet Ali 
Published: May 2021

Purpose
The purpose of this paper is to develop new four-dimensional (4D) hyperchaotic system by adding another state variable and linear controller to three-dimensional T chaotic dynamical systems. Its dynamical analyses, circuit experiment, control and synchronization applications are presented.

Design/methodology/approach
A new 4D hyperchaotic attractor is achieved through a simulation, circuit experiment and mathematical analysis by obtaining the Lyapunov exponent spectrum, equilibrium, bifurcation, Poincaré maps and power spectrum. Moreover, hardware experimental measurements are performed and obtained results well validate the numerical simulations. Also, the passive control method is presented to make the new 4D hyperchaotic system stable at the zero equilibrium and synchronize the two identical new 4D hyperchaotic system with different initial conditions.

Findings
The passive controllers can stabilize the new 4D chaotic system around equilibrium point and provide the synchronization of new 4D chaotic systems with different initial conditions. The findings from Matlab simulations, circuit design simulations in computer and physical circuit experiment are consistent with each other in terms of comparison.

Originality/value
The 4D hyperchaotic system is presented, and dynamical analysis and numerical simulation of the new hyperchaotic system were firstly carried out. The circuit of new 4D hyperchaotic system is realized, and control and synchronization applications are performed.
Reliability analysis for dependent competing failure of harmonic drive with strength failure and stiffness degradation failure
Authors: Zhang Xian , Jiang Gedong , Zhang Hao , Yun Xialun , Mei Xuesong 
Published: May 2021

PurposeThe purpose of this paper is to analyze the dependent competing failure reliability of harmonic drive (HD) with strength failure and degradation failure.Design/methodology/approachBased on life tests and stiffness degradation experiments, Wiener process is used to establish the accelerated performance degradation model of HD. Model parameter distribution is estimated by Bayesian inference and Markov Chain Monte Carlo (MCMC) and stiffness degradation failure samples are obtained by a three-step sampling method. Combined with strength failure samples of HD, copula function is used to describe the dependence between strength failure and stiffness degradation failure.FindingsStrength failure occurred earlier than degradation failure under high level accelerated condition; degradation failure occurred earlier than strength failure under medium- or low-level accelerated condition. Gumbel copula is the optimum copula function for dependence modeling of strength failure and stiffness degradation failure. Dependent competing failure reliability of HD is larger than independent competing failure reliability.Originality/valueThe reliability evaluation method of dependent competing failure of HD with strength failure and degradation failure is first proposed. Performance degradation experiments during accelerated life test (ALT), step-down ALT and life test under rated condition are conducted for Wiener process based step-down accelerated performance degradation modeling.
How social media influencer’s event endorsement changes attitudes of followers: the moderating effect of followers’ gender
Authors: Sun Jie , Leung Xi Yu , Bai Billy 
Published: May 2021

Purpose
This paper aims to study how a social media influencer (SMI) endorsement affects followers’ event attitude and behavioral intentions. More importantly, it comparatively examines the role of followers’ gender in event SMI marketing effectiveness.

Design methodology approach
Based on the congruity theory of attitude change, the current study developed an integrated framework of SMI marketing. In total, 335 online surveys were collected. Partial least squares structural equation modeling and multi-group analysis were conducted to test the hypotheses.

Findings
Results show that a follower’s attitude and behavioral intentions toward the event are affected by his or her influencer attitude. The present study also finds that follower’s social media post attitude plays a mediating role. Moreover, female followers are impacted more directly by their influencer attitude, while male followers are affected more indirectly through their attitude toward the social media posts.

Research limitations implications
The current study not only extends the event literature on SMI marketing but also provides recommendations for event professionals on SMI selection.

Originality value
This study examines SMI endorsement as a relatively new marketing tool in the event setting. It also is a pioneer study in testing the effect of social media followers’ gender differences.
Human capital interaction on foreign aid-growth nexus: evidence from South Asia and sub-Saharan Africa
Authors: Nwani Stanley Emife 
Published: May 2021

Purpose
The purpose of this study is to examine the interactive role of human capital development (HCD) in foreign aid-growth relations in South Asia and sub-Saharan Africa countries from 1985–2019.

Design/methodology/approach
The study used panel data that cut across all countries in South Asia and sub-Saharan Africa collected from The World Bank’s Development Indicators. The data were analysed using Bai and Ng panel unit root idiosyncratic cross-sectional tests and the system generalised method of moments (SGMM).

Findings
The study found that foreign aid and HCD have negative impacts on economic growth. Fortunately, the interaction of human capital with foreign aid reduces the extent to which foreign aid impedes economic growth. The presumption is that South Asia and sub-Saharan Africa economies had not reaped the potential growth effect of foreign aid inflows due to high illiteracy rates and weak social capacities. The peculiarity of these regions hinders the absorptive capacity to transform positive externality associated with foreign aid into sizeable economic prosperity.

Practical implications
It is imperative for South Asia and sub-Saharan Africa countries to not depend on foreign aid; instead, the strategic action by policymakers should be to developing sustainable social capacities with HCD as the centre-piece.

Originality/value
The highpoint of this study is its inter-regional approach and the interplay between human capital and foreign aid using the second generation panel unit root estimator and the SGMM approaches.