bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cissus Quadrangularis enhances UCP1 mRNA, indicative of white adipocyte browning and decreases central obesity in humans in a randomized trial
Authors: Chatree Saimai , Sitticharoon Chantacha , Maikaew Pailin , Pongwattanapakin Kitchaya , Keadkraichaiwat Issarawan , Churintaraphan Malika , Sripong Chanakarn , Sririwichitchai Rungnapa , Tapechum Sompol 
Published: January 2021

AbstractObesity is associated with the growth and expansion of adipocytes which could be decreased via several mechanisms. Cissus Quadrangularis (CQ) extract has been shown to reduce obesity in humans; however, its effect on human white adipocytes (hWA) has not been elucidated. This study aimed to investigate the effects of CQ on obesity, lipolysis, and browning of hWA. CQ treatment in obese humans significantly decreased waist circumference at week 4 and week 8 when compared with the baseline values (p < 0.05 all) and significantly decreased hip circumference at week 8 when compared with the baseline and week 4 values (p < 0.05 all). Serum leptin levels of the CQ-treated group were significantly higher at week 8 compared to baseline levels (p < 0.05). In hWA, glycerol release was reduced in the CQ-treated group when compared with the vehicle-treated group. In the browning experiment, pioglitazone, the PPAR-γ agonist, increased UCP1 mRNA when compared to vehicle (p < 0.01). Interestingly, 10, 100, and 1000 ng/ml CQ extract treatment on hWA significantly enhanced UCP1 expression in a dose-dependent manner when compared to pioglitazone treatment (p < 0.001 all). In conclusion, CQ decreased waist and hip circumferences in obese humans and enhanced UCP1 mRNA in hWA suggestive of its action via browning of hWA.
Effect of homogenization and solution treatments time on the elevated-temperature mechanical behavior of Inconel 718 fabricated by laser powder bed fusion
Authors: Fayed Eslam M. , Saadati Mohammad , Shahriari Davood , Brailovski Vladimir , Jahazi Mohammad , Medraj Mamoun 
Published: January 2021

AbstractIn the present study, the effect of homogenization and solution treatment times on the elevated-temperature (650 °C) mechanical properties and the fracture mechanisms of Inconel 718 (IN718) superalloy fabricated by laser powder bed fusion (LPBF) was investigated. Homogenization times between 1 and 7 h at 1080 °C were used, while solution treatments at 980 °C were performed in the range from 15 to 60 min. The as-printed condition showed the lowest strength but the highest elongation to failure at 650 °C, compared to the heat-treated conditions. After heat treatments, the strength of the IN718 alloy increased by 20.3–31% in relation to the as-printed condition, depending on the treatment time, whereas the ductility decreased significantly, by 67.4–80%. Among the heat treatment conditions, the 1 h homogenized conditions at 1080 °C (HSA1 and HSA2) exhibited the highest strength and ductility due to the combined effects of the precipitation hardening and sub-structural changes. Further increases in the homogenization time to 4 and 7 h led to a decrease in the strength and significant ductility loss of the LPBF IN718 due to the considerable annihilation of the dislocation tangles and a greater precipitation of coarse MC carbide particles. Furthermore, it was found that the solution treatment duration had a crucial influence on the mechanical properties at 650 °C due to the increase in the grain boundary strength through the pinning effect of the intergranular δ-phase. In addition, the fracture mechanism of the LPBF IN718 was found to be dependent on the heat treatment time. Finally, this investigation provides a map that summarizes the effect of homogenization and solution treatment times on the high-temperature mechanical behavior of LPBF IN718 by relating it to the corresponding microstructural evolution. This effort strives to assist in tailoring the mechanical properties of LPBF IN718 based on the design requirements for some specific applications.
Intestinal long non-coding RNAs in response to simulated microgravity stress in Caenorhabditis elegans
Authors: Sun Lingmei , Li Dan , Yuan Yujie , Wang Dayong 
Published: January 2021

AbstractLong non-coding RNAs (lncRNAs) are important in regulating the response to environmental stresses in organisms. In this study, we used Caenorhabditis elegans as an animal model to determine the functions of intestinal lncRNAs in regulating response to simulated microgravity stress. Among the intestinal lncRNAs, linc-2, linc-46, linc-61, and linc-78 were increased by simulated microgravity treatment, and linc-13, linc-14, linc-50, and linc-125 were decreased by simulated microgravity treatment. Among these 8 intestinal lncRNAs, RNAi knockdown of linc-2 or linc-61 induced a susceptibility to toxicity of simulated microgravity, whereas RNAi knockdown of linc-13, linc-14, or linc-50 induced a resistance to toxicity of simulated microgravity. In simulated microgravity treated nematodes, linc-50 potentially binds to three transcriptional factors (DAF-16, SKN-1, and HLH-30). RNAi knockdown of daf-16, skn-1, or hlh-30 could suppress resistance of linc-50(RNAi) nematodes to the toxicity of simulated microgravity. Therefore, our results provide an important basis for intestinal lncRNAs, such as the linc-50, in regulating the response to simulated microgravity in nematodes.
The impact of pipeline changes and temperature increase in a hospital historically colonised with Legionella
Authors: Quero S. , Párraga-Niño N. , Garcia-Núñez M. , Pedro-Botet M. L. , Gavaldà L. , Mateu L. , Sabrià M. , Mòdol J. M. 
Published: January 2021

AbstractHealthcare-related Legionnaires’ disease has a devastating impact on high risk patients, with a case fatality rate of 30–50%. Legionella prevention and control in hospitals is therefore crucial. To control Legionella water colonisation in a hospital setting we evaluated the effect of pipeline improvements and temperature increase, analysing 237 samples over a 2-year period (first year: 129, second year: 108). In the first year, 25.58% of samples were positive for Legionella and 16.67% for amoeba. Assessing the distance of the points analysed from the hot water tank, the most distal points presented higher proportion of Legionella colonisation and lower temperatures (nearest points: 6.4% colonised, and temperature 61.4 °C; most distal points: 50% and temperature 59.1 °C). After the first year, the hot water system was repaired and the temperature stabilised. This led to a dramatic reduction in Legionella colonisation, which was negative in all the samples analysed; however, amoeba colonisation remained stable. This study shows the importance of keeping the temperature stable throughout the circuit, at around 60 °C. Special attention should be paid to the most distal points of the circuit; a fall in temperature at these weak points would favour the colonisation and spread of Legionella, because amoeba (the main Legionella reservoir) are not affected by temperature.
Association of proportion of the HDL-cholesterol subclasses HDL-2b and HDL-3 and macrovascular events among patients undergoing hemodialysis
Authors: Lee Wen-Chin , Chen Jin-Bor , Moi Sin-Hua , Yang Cheng-Hong 
Published: January 2021

AbstractAltered high-density lipoprotein cholesterol (HDL-C) subclass distribution in hemodialysis (HD) patients is well documented. Aim of this study is to investigate the relationship between HDL-C subclass distribution and macrovascular events in patients undergoing HD. A total of 164 prevalent HD patients and 71 healthy individuals in one hospital-facilitated clinic were enrolled from May 2019 to July 2019 and individual HD patients was follow-up for one year. Macrovascular events (cerebral stroke, coronary heart disease) were recorded in the study period. The HDL-2b, HDL-3 proportions and biochemical parameters were measured. Pearson correlation test and logistic regression analysis were used to examine correlation and odds ratio (OR). 144 HD patients completed one-year follow-up. Cohort with macrovascular events revealed significantly lower HDL-2b and higher HDL-3 subclass proportions compared to those without events. By multivariable adjustment, HDL-3 subclass proportion revealed significantly increase risk for these events (OR 1.17, 95% CI 1.02–1.41, P = 0.044). HDL-2b subclass was significantly higher and HDL-3 subclass was significantly lower in the HD cohort under the hs-CRP level of < 3 mg/L compared to higher hs-CRP level. In conclusion, HDL-2b and HDL-3 subclasses distributions were associated with macrovascular events in HD patients. Proinflammatory status influences the distribution of HDL-2b and HDL-3 subclasses in HD patients.
Preferential lattice expansion of polypropylene in a trilayer polypropylene/polyethylene/polypropylene microporous separator in Li-ion batteries
Authors: Hsu Wen-Dung , Yang Po-Wei , Chen Hung-Yuan , Wu Po-Hsien , Wu Pin-Chin , Hu Chih-Wei , Saravanan Lakshmanan , Liao Yen-Fa , Su Yen-Teng , Bhalothia Dinesh , Chen Tsan-Yao , Chang Chia-Chin 
Published: January 2021

AbstractThe abnormal lattice expansion of commercial polypropylene (PP)/polyethylene (PE)/polypropylene (PP) separator in lithium-ion battery under different charging current densities was observed by in-situ X-ray diffraction. Significant lattice changes of both PP and PE were found during the low current density charging. The capacity fading and the resistance value of the cell measured at 0.025 C (5th retention, 92%) is unexpectedly larger than that at 1.0 C (5th retention, 97.3%) from the electrochemical impedance spectroscopic data. High-resolution scanning electron microscopy is employed to witness the pore changes of the trilayered membrane. Density functional theory calculations were used to investigate the mechanism responsible for the irregular results. The calculations revealed that the insertion of Li-ion and EC molecule into PP or PE are thermodynamically favourable process which might explain the anomalous significant lattice expansion during the low current density charging. Therefore, designing a new separator material with a more compact crystalline structure or surface modification to reduce the Li insertion during the battery operation is desirable.
Metagenomic analysis of the cow, sheep, reindeer and red deer rumen
Authors: Glendinning Laura , Genç Buğra , Wallace R. John , Watson Mick 
Published: January 2021

AbstractThe rumen microbiota comprises a community of microorganisms which specialise in the degradation of complex carbohydrates from plant-based feed. These microbes play a highly important role in ruminant nutrition and could also act as sources of industrially useful enzymes. In this study, we performed a metagenomic analysis of samples taken from the ruminal contents of cow (Bos Taurus), sheep (Ovis aries), reindeer (Rangifer tarandus) and red deer (Cervus elaphus). We constructed 391 metagenome-assembled genomes originating from 16 microbial phyla. We compared our genomes to other publically available microbial genomes and found that they contained 279 novel species. We also found significant differences between the microbiota of different ruminant species in terms of the abundance of microbial taxonomies, carbohydrate-active enzyme genes and KEGG orthologs. We present a dataset of rumen-derived genomes which in combination with other publicly-available rumen genomes can be used as a reference dataset in future metagenomic studies.
Sedimentary microplastic concentrations from the Romanian Danube River to the Black Sea
Authors: Pojar Iulian , Stănică Adrian , Stock Friederike , Kochleus Christian , Schultz Michael , Bradley Chris 
Published: January 2021

AbstractA multitude of recent studies have detailed microplastic concentrations in aquatic and terrestrial environments, although questions remain over their ultimate fate. At present, few studies have detailed microplastic characteristics and abundance along a freshwater–marine interface, and considerable uncertainties remain over the modelled contribution of terrestrial and riverine microplastic to the world’s oceans. In this article, for the first time, we detail sedimentary microplastic concentrations along a River–Sea transect from the lower reaches of a major continental river, the River Danube, through the Danube Delta, the Black Sea coast to the Romanian and Bulgarian inner shelf of the Black Sea. Our results indicate that isolated areas of the Danube Delta are still relatively pristine, with few microplastic particles in some of the sediments sampled.
Effects of biotin on promoting anammox bacterial activity
Authors: Li Qinyu , Chen Jinhui , Liu Guo-hua , Xu Xianglong , Zhang Qian , Wang Yijin , Yuan Junli , Li Yinghao , Qi Lu , Wang Hongchen 
Published: January 2021

AbstractAnaerobic ammonium oxidation (anammox) bacteria significantly improve the efficiency and reduce cost of nitrogen removal in wastewater treatment plants. However, their slow growth and vulnerable activity limit the application of anammox technology. In this paper, the enhancement of biotin on the nitrogen removal activity of anammox bacteria in short-term batch experiments was studied. We found that biotin played a significant role in promoting anammox activity within a biotin concentration range of 0.1–1.5 mg/L. At a biotin concentration of 1.0 mg/L, the total nitrogen removal rate (NRR) increased by 112%, extracellular polymeric substance (EPS) secretion and heme production significantly improved, and anammox bacterial biomass increased to maximum levels. Moreover, the predominant genus of anammox bacteria was Candidatus Brocadia.
N-doped graphene foam obtained by microwave-assisted exfoliation of graphite
Authors: Skorupska Malgorzata , Ilnicka Anna , Lukaszewicz Jerzy P. 
Published: January 2021

AbstractThe synthesis of metal-free but electrochemically active electrode materials, which could be an important contributor to environmental protection, is the key motivation for this research approach. The progress of graphene material science in recent decades has contributed to the further development of nanotechnology and material engineering. Due to the unique properties of graphene materials, they have found many practical applications: among others, as catalysts in metal-air batteries, supercapacitors, or fuel cells. In order to create an economical and efficient material for energy production and storage applications, researchers focused on the introduction of additional heteroatoms to the graphene structure. As solutions for functionalizing pristine graphene structures are very difficult to implement, this article presents a facile method of preparing nitrogen-doped graphene foam in a microwave reactor. The influence of solvent type and microwave reactor holding time was investigated. To characterize the elemental content and structural properties of the obtained N-doped graphene materials, methods such as elemental analysis, high-resolution transmission electron microscopy, scanning electron microscopy, and Raman spectroscopy were used. Electrochemical activity in ORR of the obtained materials was tested using cyclic voltamperometry (CV) and linear sweep voltamperometry (LSV). The tests proved the materials’ high activity towards ORR, with the number of electrons reaching 3.46 for tested non-Pt materials, while the analogous value for the C-Pt (20 wt% loading) reference was 4.
A cross-species assessment of behavioral flexibility in compulsive disorders
Authors: Benzina Nabil , N’Diaye Karim , Pelissolo Antoine , Mallet Luc , Burguière Eric 
Published: January 2021

AbstractLack of behavioral flexibility has been proposed as one underlying cause of compulsions, defined as repetitive behaviors performed through rigid rituals. However, experimental evidence has proven inconsistent across human and animal models of compulsive-like behavior. In the present study, applying a similarly-designed reversal learning task in two different species, which share a common symptom of compulsivity (human OCD patients and Sapap3 KO mice), we found no consistent link between compulsive behaviors and lack of behavioral flexibility. However, we showed that a distinct subgroup of compulsive individuals of both species exhibit a behavioral flexibility deficit in reversal learning. This deficit was not due to perseverative, rigid behaviors as commonly hypothesized, but rather due to an increase in response lability. These cross-species results highlight the necessity to consider the heterogeneity of cognitive deficits in compulsive disorders and call for reconsidering the role of behavioral flexibility in the aetiology of compulsive behaviors.
Evidence-Based Practice in Speech-Language Pathology: Where Are We Now?
Authors: Greenwell Tamar , Walsh Bridget 
Published: January 2021

Purpose
In 2004, American Speech-Language-Hearing Association established its position statement on evidence-based practice (EBP). Since 2008, the Council on Academic Accreditation has required accredited graduate education programs in speech-language pathology to incorporate research methodology and EBP principles into their curricula and clinical practicums. Over the past 15 years, access to EBP resources and employer-led EBP training opportunities have increased. The purpose of this study is to provide an update of how increased exposure to EBP principles affects reported use of EBP and perceived barriers to providing EBP in clinical decision making.

Method
Three hundred seventeen speech-language pathologists completed an online questionnaire querying their perceptions about EBP, use of EBP in clinical practice, and perceived barriers to incorporating EBP. Participants' responses were analyzed using descriptive and inferential statistics. We used multiple linear regression to examine whether years of practice, degree, EBP exposure during graduate program and clinical fellowship (CF), EBP career training, and average barrier score predicted EBP use.

Results
Exposure to EBP in graduate school and during the CF, perception of barriers, and EBP career training significantly predicted the use of EBP in clinical practice. Speech-language pathologists identified the three major components of EBP: client preferences, external evidence, and clinical experience as the most frequently turned to sources of EBP. Inadequate time for research and workload/caseload size remain the most significant barriers to EBP implementation. Respondents who indicated time was a barrier were more likely to cite other barriers to implementing EBP. An increase in EBP career training was associated with a decrease in the perception of time as a barrier.

Conclusions
These findings suggest that explicit training in graduate school and during the CF lays a foundation for EBP principles that is shaped through continued learning opportunities. We documented positive attitudes toward EBP and consistent application of the three components of EBP in clinical practice. Nevertheless, long-standing barriers remain. We suggest that accessible, time-saving resources, a consistent process for posing and answering clinical questions, and on the job support and guidance from employers/organizations are essential to implementing clinical practices that are evidence based. The implications of our findings and suggestions for future research to bridge the research-to-practice gap are discussed.
W‐band TE102‐mode filter with doubly loaded E‐plane and H‐plane irises
Authors: Bartlett Chad , Höft Michael 
Published: January 2021

AbstractIn this correspondence, high‐precision computer numerical control milling is utilised to demonstrate a doubly loaded iris, cross‐coupled waveguide bandpass filter for inline operation within the W‐band. This sixth‐order filter is designed in a stacked H‐plane configuration and utilises both E‐plane and H‐plane doubly loaded irises to maintain the flow of a TE102‐mode electromagnetic field. In this configuration, a high rejection level is maintained outside of the passband while a good unloaded quality factor is obtained through the support of larger resonator dimensions. The filter is designed for approximately 2% fractional bandwidth centred at 97.5 GHz and has been fabricated as three brass components. Measurements of the filter agree very well with the simulated results and demonstrate a spurious‐free design over the full W‐band.
Five‐level class‐D amplifier employing fourth‐order continuous‐time sigma‐delta modulator
Authors: Pakniat Hossein 
Published: January 2021

AbstractA new closed‐loop multi‐level Class‐D amplifier employing a fourth‐order ‘continuous‐time sigma‐delta modulator’ is proposed and analysed. The proposed amplifier is intrinsically linear and it has approximately the fixed ‘total harmonic distortion plus noise’ against the input frequency. The corresponding circuit is designed and simulated in 180 nm technology (3.3 V), while the post‐layout model of the power stage is employed. A total harmonic distortion plus noise of 0.0016%, the quiescent current of 2.6 mA, the maximum efficiency of 94%, the average switching frequency of 400∼500 kHz and an excellent ‘power supply reject ration’ of 120 dB are achieved.
Demonstration of a 20‐W membrane‐external‐cavity surface‐emitting laser for sodium guide star applications
Authors: Priante D. , Zhang M. , Albrecht A.R. , Bek R. , Zimmer M. , Nguyen C. , Follman D. , Cole G.D. , Sheik‐Bahae M. 
Published: January 2021

AbstractWe present a record 20‐W continuous‐wave output power membrane‐external‐cavity surface‐emitting laser (MECSEL) double‐bonded to silicon carbide (SiC) heat spreaders emitting around 1178 nm. We achieved a maximum slope efficiency of 27.7% and an optical‐to‐optical efficiency of 26.7%. By utilising an intracavity birefringent filtre, the wavelength was tuned over 75 nm and maximum output power of 12.7 W was recorded at 1178 nm. This study shows the potential of MECSELs to achieve high‐performance and cost‐effective optically pumped semiconductor disk lasers targeting applications in sodium guide stars.
Enhancement of sensing range of Brillouin optical time‐domain reflectometry system up to 150 km with in‐line bi‐directional erbium‐doped fibre amplifications
Authors: Clement Pierre , Gabet Renaud , Lanticq Vincent , Jaouën Yves 
Published: January 2021

AbstractBrillouin optical time‐domain reflectometry (BOTDR) has the advantage of requiring access to only one end of the sensing fibre, which is an important property for some applications. The coherent detection of spontaneous Brillouin scattering allows high sensitivity of temperature/strain measurements, close to one of the well‐known Brillouin optical time‐domain analyser‐based solutions. In‐line amplification based on bidirectional erbium‐doped fibre amplification (EDFA) modules can improve sensing distance and temperature accuracy. This study demonstrates a distributed sensing over 150 km with 5‐m spatial resolution and a temperature error of less than 2°C in 10 min without requiring specific BOTDR adaptation. A record performance of 0.7°C in 60 min has been also obtained, which corresponds to the best performance obtained in reflectometer configuration at this distance.