bluuebunny/crossref_metadata_2025_split huggingface dataset:
Risk Factors of Early Postoperative Bowel Obstruction for Patients Undergoing Selective Colorectal Surgeries
Authors: Yang Shuguang , Gao Zhidong , Zhao Huiying , An Youzhong , Yang Jianghui , Zhang Hua , Bao Yudi , Ye Yingjiang 
Published: August 2021

Abstract
Objective: Postoperative bowel obstruction was one of the most severe complications in patients received colorectal surgeries. The aim of this study was to explore risk factors of early postoperative obstruction and to construct a nomogram to predict the possibility of occurrence. Methods: The records of 1437 patients who underwent selective colorectal surgery in Peking University People’s Hospital from 2015 to 2020 was retrospectively collect. Risk factors of early postoperative bowel obstruction were identified by logistic regression analysis and a nomogram was then constructed. Bootstrap was applied to verify the stability of the model. Results: COPD, hypothyroidism, probiotic indications, duration of antibiotics, and time to postoperative feeding were identified as independent risk factors and were put into a nomogram for predicting early postoperative bowel obstruction. The nomogram showed a robust discrimination, with area under the receiver operating characteristic curve was 0.894 and was well calibrated. Conclusion: A nomogram including independent risk factors of COPD, hypothyroidism, probiotic indications, duration of antibiotics, and time to postoperative feeding were established to predict the risk of early postoperative bowel obstruction.
Effectiveness of Negative Pressure Wound Therapy With Instillation and Dwelling After Stoma Closure: A Retrospective and Propensity Score Matching Analysis
Authors: Yane Yoshinori , Hida Jin-ichi , Chiba Yasutaka , Makutani Yusuke , Ushijima Hokuto , Yoshioka Yasumasa , Iwamoto Masayoshi , Wada Toshiaki , Daito Koji , Tokoro Tadao , Ueda Kazuki , Kawamura Junichiro 
Published: August 2021

Abstract
The use of temporary diverting stoma has become more common in low colorectal anastomosis to reduce anastomotic complications. Surgical site infection (SSI) at the stoma closure site has been one of the most frequent postoperative complications. The aim of this study was to compare the short-term outcomes between conventional primary suture closure and negative pressure wound therapy with instillation and dwelling (NPWTi-d) therapy following purse-string suturing, using propensity score matching analysis. We retrospectively evaluated the medical records of 107 patients who underwent stoma closure between January 2016 and October 2020. The primary outcome was the proportion of SSI. The secondary outcome was the day of postoperative length of stay. Propensity score matching with one-to-one match was performed for reducing treatment selection bias. Of a total of 107 patients, 67 patients had been treated with conventional primary closure and 40 with NPWTi-d therapy. The propensity score matching derived 37 pairs. The respective SSI proportions were 0% and 16.2% in the groups with NPWTi-d and primary closure (P=0.025). The respective median days of postoperative hospital stay were 9.0 and 10.0 in the groups with NPWTi-d and primary closure (P=0.453). NPWTi-d therapy with purse-string suturing was effective in reducing SSI after stoma closure.
An Integrated Analysis of The Prognosis And Immune Cell Infiltration of ITGB Superfamily Members In Gastric Cancer
Authors: Li Chuan-hong , Meng Lei , Chen Zhang-ming , Sui Wan-nian , Chen Pei-feng , Lin Ming-gui , Ying Song-cheng , Han Wen-xiu 
Published: August 2021

Abstract
Background:Members of the integrin β superfamily(ITGBs) have been shown to be aberrantly expressed in various human cancers and involved in tumorigenesis and progression. However, the diverse expression patterns and prognostic values of the entire ITGB family members in gastric cancer(GC) has not been systematically investigated.Methods:In the current study, Oncomine, GEPIA, Kaplan Meier plotter, TIMER, GeneMANIA, STRING and Metascape database were employed to explore the transcriptional and survival data of ITGB superfamily members in GC. Moreover, we confirmed the mRNA expression levels of ITGB superfamily members in GC cell lines by qRT-PCR.Results:The mRNA expression level of ITGB1/2/4/5/8 was upregulated in GC, while the expression level of ITGB7 was downregulated. Higher expression of ITGB2/7 was significantly associated with the tumor stage of patients with GC. However, we found that the expression level of ITGB1/2/4/5/6/7/8 was remarkably increased in GC cell lines compared to stomach normal cell lines, while ITGB3 expression was decreased in the former than in the latter. Meanwhile,higher expression levels of ITGB2/6/7 were closely correlated with better overall clinical survival (OS) and recurrence-free survival (RFS) in GC patients, while higher ITGB3/4/5 expression were strongly associated with poorer OS and RFS.We also discovered that the functions of ITGBs and their adjacent genes are mainly related to protein complexes involved in cell adhesion. the functions of ITGBs and their adjacent proteins are mainly related to focal adhesion, cell adhesion molecules, proteoglycans in cancer, small cell lung cancer, rap1 signaling pathway, IgA production by intestinal immune network, and microRNAs in cancer.In addition, the expression of ITGBs was significantly correlated with the infiltration of multiple immune cells, including B cells, CD8+ T cells, CD4+ T cells, neutrophils, macrophages, and dendritic cells.Conclusions:Our results suggested that abnormal expression of ITGBs plays a key role in the progression of GC and that ITGBs may be potential prognostic biomarkers and therapeutic targets for GC.
Microspheres and Nanorods Produced in the Dissolution of Mildly Carboxylated Cellulose Fibers in Alkaline Solutions
Authors: Moradian Mohammadhadi , de Ven Theo G.M. G.M. van 
Published: August 2021

Abstract
A mild etherification of spruce kraft pulp was performed to introduce 1.3 and 2.5 mmol/g carboxyl groups on cellulose chains. 1.3 mmol/g carboxymethyl fibers (CMF) were dissolved partially in alkaline water to form balloons and collars on the tracheid and their ultra-structure was investigated. Primary wall, expanded S1, swollen S2, wrinkled S3, spiral bands of S1, parallel microfibrils of S2 and their transverse splitting were observed on swollen fibers. It is indicated that balloons, collars and wrinkled S3 were formed due to different cellulose microfibril features in different layers of tracheid cell wall. Microspheres with a size up to about 0.6 µm were observed by field emission scanning electron microscopy (FE-SEM) and transmission electron microscopy (TEM). It is shown that they originated from transverse splitting of S2 microfibrils and contain bundles of well-known cellulose nanocrystals (CNC). After homogenization and sonication of an aqueous dispersion of 2.5 mmol/g CMF, electroacoustic spectroscopy showed the presence of nanorods with a size distribution of 18-208 nm. Similar sizes were observed by TEM.
Investigating the existence of asymmetric Environment Kuznets Curve and Pollution-Haven Hypothesis in China: Fresh evidence from QARDL and Quantile Granger Causality
Authors: Akram Rabia , Fareed Zeeshan , Xiaoli Gan , Zulfiqar Bushra , Shahzad Farrukh 
Published: August 2021

Abstract
This study aims to analyze the asymmetric long-run relationship between economic growth (EG), Foreign Direct Investment (FDI) and carbon emissions (CO2) within the context of the Environment Kuznets curve (EKC) and the pollution haven hypothesis (PHH) in China. Employing the quarterly data from 1982Q1 to 2018Q4, we have used novel techniques to meet the stated objectives of our study, named Quantile ARDL and quantile Granger causality. The significant implication of this method is that it provides locational asymmetry. We find strong evidence of the EKC and PHH for China based on the empirical results of linear and nonlinear ARDL models. Similarly, findings of quantile Granger causality validate the bidirectional relationship among all variables in upper and lower quantiles. Moreover, the results of the Wald test confirm the asymmetric long-run relationship between FDI and carbon emissions (CO2). This study will be conducive for the policymakers to combat environmental contamination concerning economic growth and FDI inflow in China.
Heterogeneous Analysis of Pollution Abatement via Renewable and Non-Renewable Energy: Lessons from Investment in G20 Nations
Authors: Ajide Kazeem Bello , Mesagan Ekundayo Peter 
Published: August 2021

Abstract
This study analyses the role of renewable and non-renewable energy in pollution reduction through the capital investment channel in G20 economies between 1990 and 2017. We consider cross-sectional dependence since the countries are heterogeneous and cross-sectionally dependent using the pooled mean group approach. Findings reveal that renewable energy negatively impacts carbon emissions in both the short- and long-run, while non-renewable energy positively affects carbon emissions in both the short- and long-run. Again, results show that capital investment lowers pollution in the short-run but increases it in the long-run. Lastly, we find that capital investment interacts with renewable energy to reduce pollution in both short- and long-run, while its interaction with non-renewable energy expands pollution in both short- and long-run. We, therefore, conclude that capital investment provides an important channel to reduce pollution in G20 nations and recommend that if energy consumption is to work through the capital investment channel to lower pollution in the G20, the proportion of renewable energy must increase relative to non-renewable energy in their energy mix.JEL Classification: Q41; Q42; Q53; F23; O50.
Gene Co-Expression Network Analysis Confirms Four Independent Predictors of Survival In Ovarian Cancer Patients Under Optimal Debulking Surgery
Authors: Zhang Cong , Zhu Tao , Hu Ting , Sun Qian 
Published: August 2021

Abstract
Background: Serious ovarian cancer (OvCa) is the most common histological type of epithelial OvCa with poor prognosis. Despite received optimal cytoreduction and standard chemotherapy, a large proportion of patients are forced to recurrence or death within three years. To identify exact prognostic biomarkers associated with overall survival (OS) is urgent requirements of exploring rapid tumor progression mechanisms and developing novel strategies for immunotherapy.Methods: The gene expression profiles of GSE49997, GSE9891 and TCGA were screened through rigorous criteria using R software and Bioconductor package. Weighted gene co-expression network analysis (WGCNA) was constructed to figure out gene clusters associated with OS. Protein-protein interaction (PPI) networks were built through STRING website. Prognostic values of potential biomarkers were validated using forest map and Kaplan-Meier analysis.Results: According to screening criteria, 788 samples and 10402 genes were reserved as the modeling dataset. We detected five modules related to OS and intersected 108 genes through WGCNA after random sampling. PPI network analysis, Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis revealed potential mechanisms of above biomarkers. Conclusions: Four exact biomarkers (CANT1, P4HB, DUS1L and SIRT7) were confirmed as independent predictors of survival in OvCa patients with success of debulking surgery, which might provide promising biomarkers for prognostic judgement in ovarian cancer.
LINC01272 Activates Epithelial-Mesenchymal Transition Through miR-153-5p in Crohn’s Disease
Authors: Fang Lin , Hu Mengcheng , Xia Fei , Bai wenxia 
Published: August 2021

Abstract
Background: Long non-coding RNAs (lncRNAs) have different functions in different diseases. There is seldom research on the functions of lncRNAs in Crohn’s disease (CD). By RNA-seq technology, we identify a lncRNA associated with Crohn's disease. However, the mechanism of lncRNA regulation remains unknown. This study aimed to determine the association of LINC01272 with epithelial cell-mesenchymal transition and the underlined mechanism in CD.Methods: RNA is detected by qRT-PCR. Interaction of protein and RNA was determined by RNA binding protein immunoprecipitation. Luciferase reporter assays were used to detect the targeted miRNA of LINC01272. Tissue fibrosis was observed by Masson and HE staining. The protein expression is determined by western blot and immunofluorescence. Results: LINC01272 was highly expressed in patients with CD. Knockdown of LINC01272 inhibited TGF-β1-induced epithelial-mesenchymal transition (EMT). Additionally, LINC01272 regulated TGF-β1 induced EMT by miR-153-5p axis and knockdown of LINC01272 inhibited EMT in the CD mice in vivo. Conclusion: LINC01272 activated epithelial-mesenchymal transition through miR-153-5p in CD.
Carryover of a saddle-node bifurcation after transforming a parameter into a variable
Authors: Contreras Carlos , Carrero Gustavo , de Vries Gerda 
Published: August 2021

Abstract
In this article, we introduce and study the carryover of a saddle-node bifurcation, a concept that describes how a saddle-node bifurcation of a dynamical system is carried over into an extended dynamical system obtained by transforming one of the parameters of the original system into a variable. We show that additional transversality and singularity conditions are needed to guarantee the carryover of a saddle-node bifurcation and provide a graphical methodology with a two-parameter bifurcation diagram to verify that such conditions are met. The results are applied to a gene activation model when the parameter describing the signal for activation is transformed into a variable, and to a cell cycle regulatory model when the parameter describing the cell mass is transformed into a variable. In both cases, we show that a saddle-node bifurcation carryover takes place.
Exome Sequencing of Glioblastoma-Derived Cancer Stem Cells Reveals Rare Clinically Relevant Frameshift Deletion in MLLT1 Gene
Authors: Marei Hany , Althani Asmaa , Afifi Nahla , Hasan Anwarul , Caceci Thomas , Felsani Armando , Tringali Giuseppe , Pozzoli Giacomo , Cenciarelli Carlo 
Published: August 2021

Abstract
Background: Glioblastoma multiforme (GBM) is a heterogeneous CNS neoplasm which causes significant morbidity and mortality. One reason for the poor prognostic outcome of GBM is attributed to the presence of cancer stem cells (CSC) which confer resistance against standard chemo- and radiotherapeutics modalities. Two types of GBM-associated CSC were isolated from the same patient: tumor core- (c-CSC) and peritumor tissue-derived cancer stem cells (p-CSC).Methods: In the present study, potential differences in genetic variants between c-CSC versus p-CSC derived from four GBM patients were investigated with the aims of 1) comparing the exome sequences between all the c-CSC or p-CSC to identify the common variants; 2) identifying the variants affecting the function of genes known to be involved in cancer origin and development.Results: By comparative analyses, we identified common gene single nucleotide variants (SNV) in all GBM c-CSC and p-CSC, a potentially deleterious variant was a frameshift deletion at Gln461fs in the MLLT1 gene, that was encountered only in p-CSC samples with different allelic frequency.Conclusions: Our study supports the hypothesis that the varied genetic composition of GBM-associated c-CSC and p-CSC may be involved in different therapeutic responses or the recurrent nature of GBM.
Protective effects of theaflavins and epigallocatechin gallate against ZnO-NP-induced apoptosis in rat tracheal epithelial cells
Authors: Shan Xiaodong , Chen Feifei , Lin Huikang , Zhang Hangjun , Zhong Yuchi , Liu Zhiquan , Li Yan 
Published: August 2021

Abstract
Zinc oxide nanoparticles (ZnO-NPs) can affect human health primarily via inhalation. This study evaluated the protective effects of theaflavins (TFs) and epigallocatechin gallate (EGCG) against ZnO-NP-induced cytotoxicity in rat tracheal epithelial (RTE) cells. After exposure to ZnO-NPs (100 µg/L), treatment with TFs and EGCG (10, 100 and 1000 µg/L) significantly inhibited the levels of reactive oxygen species (ROS), and the content of malondialdehyde (MDA). Treatment also alleviated apoptosis induced by oxidative stress, which was achieved by inhibiting cytochrome C (CytoC) and Caspase 3/8/9 mRNA expression. Upon treatment with the highest concentrations of TFs and EGCG (1000 µg/L), CytoC gene expression was downregulated by 59.10% and 77.27%, Caspase 3 gene expression by 50.03% and 60.01%, Caspase 8 gene expression by 45.11% and 55.57%, and Caspase 9 gene expression by 51.33% and 66.67%. In addition, about the interleukin family as interleukin 1β (IL-1β) and interleukin 6 (IL-6), tumor necrosis factor-α (TNF-α) and the other inflammatory chemokines like C-C motif chemokine 2 (CCL2), C-X-C motif chemokine 8 (CXCL8) were upregulated in RTE cells in the presence of ZnO-NPs. All factors were gradually rescued after the addition of TFs and EGCG. These results showed that TFs and EGCG could effectively protect RTE cells from oxidative damage induced by ZnO-NPs through antiapoptotic and antioxidant effects.
Corticosteroid Sparing Agents in Frequent Relapsing and Steroid Dependent Nephrotic Syndrome in Children: A Single Center Retrospective Study
Authors: Alabbasi Bashair , Abdulmajeed Naif , Alghamdi Abdulmonem , Alzahrani Saeed , Alghwery Saeed , Aloufi Majed 
Published: August 2021

Abstract
Background: Children with frequently relapsing nephrotic syndrome (FRNS) or Steroid-dependent nephrotic syndrome (SDNS) may be prescribed non-corticosteroid immunosuppressive agents whenever there is a failure to maintain remission with low-dose alternate-day prednisone and/or signiﬁcant adverse effects of prednisone develop. A wide variety of immunosuppressive agents have been used in these patients to reduce the number of relapses and maintain remission. Objective: to evaluate the outcome of Steroid sparing agents in the management of FRNS and SDNS in children with nephrotic syndrome. Patients and methods: A retrospective study was conducted on all steroid-sensitive nephrotic syndrome (SSNS) children (1-11 years) who received any type of second line agents (e.g. CNI, MMF, cyclophosphamide, and Rituximab) over a period of 9 years from January 2010 to January 2019 in pediatric nephrology unit in Prince Sultan Military Medical City, Riyadh. Results: The study included 24 patients. Their age at diagnosis ranged between 1 and 11 years with a mean of 3.8 years and standard deviation of (±) 2.6 years. During the first year of steroid therapy, relapse occurred among 87% of patients; of them, the number of relapses being 4 or more in 21.7%. Regarding indication for the second line of treatment, SDNS was the most frequent reported (60.9%), followed by FRNS (30.4%). Concerning agents used in the second line, MMF ranked first (58.4%), followed by Cyclophosphamide (33.3%). Number of relapses after starting steroid sparing agent was more than once among 41.7% of patients. Duration of remission after starting steroid sparing agent ranged between 2 and 72 months (14±14.1). Overall response to the second line of treatment was observed among majority of patients (91.7%). Renal biopsy was performed in 45.8% of patients. Concerning side effects of steroid sparing agents, electrolytes disturbances and hypertension were reported by two (8.3%) and one (4.2%) patients respectively. Duration of remission was significantly longer among patients treated with cyclosporine (48±33.9 months) compared to other lines of treatment, p<0.001. On the other hand, hypertension was only reported among patients treated with cyclosporine, p=0.003. Conclusion: The overall response of children with SDNS and FRNS to the second line agents was significant, with favorable longer remission free period with Cyclosporine use with no major side effects. Our results affected by the retrospective design of the study, as well as the small sample size. Therefore larger scale study with prospective design is highly encouraged.
Integrated Assessment of Urban Land Carrying Capacity (ULCC) for Reducing Earthquake Risk Risaster in Palu City
Authors: Erwindy Jossi , Asdak Chay , Suganda Bombom Rachmat , Hadian Mohamad Sapari Dwi 
Published: August 2021

Abstract
The land is a natural resource that has limitations to accommodate human activities. Rapid urban population growth, continuous expansion of urban scale, rapid socioeconomic development, and increased pressure on land resources between residents and urban land are monumental contradictions when urban planning does not match land carrying capacity. Assessing urban land carrying capacity is very important to evaluate and obtain an overview of the land capability level by classifying its capacity to be designed according to the area function; to get an overview of the potential and constraints of each land capability class, and to serve as a basis for future regional development. This research was conducted in Palu City, a national urban area in Indonesia. It has limited regional development because it is an area prone to high earthquake disasters. Developing the area requires assessing the land's carrying capacity, especially to minimize the risk of earthquake hazards. The assessment involves three stages of analysis, namely Mapping Earthquake-Prone Areas with Mapping of Earthquake-Prone Areas with seismic micro-zonation; Land Capability Assessment; and Comparative Analysis of Land Capability and City Planning of Palu 2030. This study's results indicate that 74.56% of Palu City is an earthquake-prone area, dominated by land capability classes type A to B, namely low to very low land capability classes (55.42%). Thus, there are physical limitations in urban development. However, suppose it is integrated with the spatial plan of Palu City until 2030. In that case, most (56.07%) are already in accordance with the carrying capacity of their land, especially in protected areas. However, land development still does not comply with their carrying capacity (35%) in cultivation areas with earthquakes. High and covering an area of 24% of the total area of Palu City requires special attention in the development of its area going forward. The requirement that land use plans that do not comply with their carrying capacity must be strictly controlled, especially in high disaster-prone areas.