bluuebunny/crossref_metadata_2025_split huggingface dataset:
Circ-GALNT16 restrains colorectal cancer progression by enhancing the SUMOylation of hnRNPK
Authors: Peng Chaofan , Tan Yuqian , Yang Peng , Jin Kangpeng , Zhang Chuan , Peng Wen , Wang Lu , Zhou Jiahui , Chen Ranran , Wang Tuo , Jin Chi , Ji Jiangzhou , Feng Yifei , Tang Junwei , Sun Yueming 
Published: August 2021

Abstract
Background
Recent studies have investigated the role of circular RNAs (circRNAs) as significant regulatory factors in multiple cancer progression. Nevertheless, the biological functions of circRNAs and the underlying mechanisms by which they regulate colorectal cancer (CRC) progression remain unclear.

Methods
A novel circRNA (circ-GALNT16) was identified by microarray and qRT-PCR. A series of in vitro and in vivo phenotype experiments were performed to investigate the role of circ-GALNT16 in CRC. The FISH, RNA pulldown assay, RIP assay, RNA sequencing, coimmunoprecipitation, and ChIP were performed to investigate the molecular mechanisms of circ-GALNT16 in CRC progression.

Results
Circ-GALNT16 was downregulated in CRC and was negatively correlated with poor prognosis. Circ-GALNT16 suppressed the proliferation and metastatic ability of CRC cells in vitro and in vivo. Mechanistically, circ-GALNT16 could bind to the KH3 domain of heterogeneous nuclear ribonucleoprotein K (hnRNPK), which promoted the SUMOylation of hnRNPK. Additionally, circ-GALNT16 could enhance the formation of the hnRNPK-p53 complex by facilitating the SUMOylation of hnRNPK. RNA sequencing assay identified serpin family E member 1 as the target gene of circ-GALNT16 at the transcriptional level. Rescue assays revealed that circ-GALNT16 regulated the expression of Serpine1 by inhibiting the deSUMOylation of hnRNPK mediated by SUMO-specific peptidase 2 and then regulating the sequence-specific DNA binding ability of the hnRNPK-p53 transcriptional complex.

Conclusions
Circ-GALNT16 suppressed CRC progression by inhibiting Serpine1 expression through regulating the sequence-specific DNA binding ability of the SENP2-mediated hnRNPK-p53 transcriptional complex and might function as a biomarker and therapeutic target for CRC.
Women empowerment, Land Tenure and Property Rights, and household food security among smallholders in Nigeria
Authors: Kehinde Mojisola Olanike , Shittu Adebayo Musediku , Adeyonu Abigail Gbemisola , Ogunnaike Maria Gbemisola 
Published: August 2021

Abstract
Background
Empowering women, land tilting to enhance the security of Land Tenure and Property Rights (LTPRs) in agriculture vis-a-vis food and nutrition security are crucial in the pursuit of the Sustainable Development Goals. The main goal of this paper is to examine the crucial roles of women’s empowerment and LTPRs as they affect household food security among smallholder farmers in Nigeria.

Methodology
Cross-sectional data were obtained from 1152 maize and rice farmers, selected by multistage random sampling across 192 communities, 16 States and the six geopolitical zones in Nigeria. The data were collected on households’ socio-economics, food security situations, empowerment and LTPRs on parcels cultivated during the 2016/17 farming season by interviewing the adult members of the farmers’ households. HFS was assessed using the United States Department of Agriculture’ HFS Survey Module and Food and Agriculture Organization guidelines for measuring Household Dietary Diversity Score. LTPRs were measured in terms of tenure type and title registration to farmlands. HFS modelling was within the framework of Poisson, Instrumental Variable Poisson (IVP) and Zero-inflated Poisson (ZIP) regression methods, with endogeneity concerns and choice of specification addressed within Hausman specification tests.

Results
The results of the study show that households that have a share of farmland on purchase and also participate in off-farm activities are likely to be certainly food-secure in all regards. Crop diversity, households that cultivate maize only, the share of farmland on purchase and access to extension contact significantly reduce the severity of food insecurity while an increase in farm size increases the severity of food insecurity. Similarly, IV Poisson and ZIP Count results show that increase in the farm size results in the severity of food insecurity. The evidence with respect to women’s empowerment reveals that gender parity and female achievement in group membership, income control, as well as workload; reduce the extent of food insecurity among the farming households in Nigeria. We, however, discover that the female achievement in the productive decision and credit increases the severity of food insecurity among the smallholder farmers. The analyses also reveal that education of the household head, female achievement in the asset; group membership and workload are the major factors that positively influenced household dietary diversity.

Conclusion
The study suggests the adoption of climate-smart practices to overcome the issue of marginal farmlands, promotion of crop and livelihood diversification among the smallholder farmers, effective extension services delivery, and improving women’s access to productive resources to enhance household food security status in Nigeria.
An anthropogenic model of cardiovascular system adaptation to the Earth’s gravity as the conceptual basis of pathological anthropology
Authors: Belkaniya G. S. , Dilenyan L. R. , Konkov D. G. , Wsol A. , Martusevich A. K. , Puchalska L. G. 
Published: August 2021

AbstractApplying human biological evolution to solve topical problems of medicine and preventive cardiology was inspired by the realization of the need for clinical and experimental studies of biological (evolutionary) prerequisites in the occurrence of a pathology. Although it has been stated that there is a need to provide a full biological understanding of features, including those that increase an animal’s vulnerability to diseases, unfortunately, in this regard, erectile and associated adaptations to the Earth’s gravity in their physiological and pathological manifestations have not been considered. At the same time, it should be noted that humans, unlike other animal species, have the greatest vulnerability of the cardiovascular system (CVS). The latter is associated with fundamental differences in the functioning and regulation of the CVS by the influence of gravity on blood circulation in humans as upright creatures. Based on a review of comparative physiological, ontogenetic, and clinical studies from an evolutionary perspective, the idea of adaptation to the Earth’s gravity when walking upright in humans is justified as an anthropogenic basis for the physiology and pathology of the cardiovascular system and hemodynamic support systems (physio-anthropology and pathological anthropology).
Infant weight gain and DXA-measured adolescent adiposity: data from the Japan Kids Body-composition Study
Authors: Fujita Yuki , Kouda Katsuyasu , Ohara Kumiko , Nakamura Harunobu , Nakama Chikako , Nishiyama Toshimasa , Iki Masayuki 
Published: August 2021

Abstract
Background
Rapid weight gain in early life is associated with adiposity later in life. However, there is limited information on the association between weight gain and body fat mass measured using precise methods. This study aimed to investigate whether weight gain is associated with body fat mass measured by dual-energy X-ray absorptiometry (DXA) in adolescents.

Methods
Participants of this retrospective cohort study were 423 adolescents born at full-term who were enrolled in the Japan Kids Body-composition Study. Anthropometric measurements related to pregnancy, delivery, and child health were obtained from the Japanese Maternal and Child Health Handbook. Fat mass in adolescents was measured with a DXA scanner. Weight gain was defined as the change in body weight from birth to age 1.5 years. Associations between birthweight and fat mass, and between weight gain and fat mass, were evaluated using multiple regression analysis.

Results
There was a significant positive association between weight gain from birth to age 1.5 years and fat mass in adolescents (boys: standardized regression coefficient (β) = 0.253, p < 0.01; girls: β = 0.246, p < 0.01), but not between birthweight standardized for gestational age and fat mass.

Conclusion
Children with a greater change in weight from birth to age 1.5 years tended to have increased fat mass in adolescence. Weight gain in early life has a greater impact on fat mass in adolescents than birthweight.
Furniture and television tip-over injuries to children treated in United States emergency departments
Authors: Lu Chang , Badeti Jaahnavi , Mehan Tracy J. , Zhu Motao , Smith Gary A. 
Published: August 2021

Abstract
Background
Furniture and television tip-over injuries are an important source of injury to children, especially those younger than 6 years old. A current epidemiologic evaluation of tip-over injuries is needed, especially considering changes in the voluntary safety standard for clothing storage units (CSUs) and the shift in the consumer market from cathode ray tube to flat-screen televisions (TVs), and a decline in household TV ownership during recent years. The objective of this study is to update our understanding of the epidemiologic characteristics and trends of furniture (especially CSU) and TV tip-over injuries treated in United States emergency departments among children < 18 years old.

Methods
This study retrospectively analyzed data from the National Electronic Injury Surveillance System from 1990 to 2019. Trends in population-based rates were evaluated with regression techniques. All numbers of cases are expressed as national estimates.

Results
An estimated 560,203 children < 18 years old were treated in United States emergency departments for furniture or TV tip-over injuries during the 30-year study period, averaging 18,673 children annually. CSUs were involved in 17.2% (n = 96,321) of tip-overs, and TVs accounted for 41.1% (n = 230,325), which included 16,904 tip-overs (3.0%) that involved both a CSU and TV. The rate of furniture and TV tip-over injuries among children < 18 years old increased by 53.8% (p < 0.0001) from 1990 to 2010, and then decreased by 56.8% (p < 0.0001) from 2010 to 2019. Almost half (47.0%) of injuries occurred to the head/neck; 3.4% of children were admitted to the hospital. Children < 6 years old accounted for 69.9% of furniture and TV tip-over injuries overall; they accounted for 82.5% of CSU-related and 74.7% of TV-related tip-over injuries.

Conclusions
Despite the decline in tip-over injuries since 2010, more should be done to prevent these injuries, especially among children < 6 years old, because the number of injuries remains high, outcomes can be life-threatening, and effective prevention strategies are known. Safety education, warning labels, and promotion and use of tip restraint devices, while important, are not a substitute for strengthening and enforcing the stability requirements for CSUs and TVs.
Community Outpatient Psychotherapy Engagement Service for Self-harm (COPESS): a feasibility trial protocol
Authors: Saini Pooja , Hunt Anna , Taylor Peter , Mills Catherine , Clements Caroline , Mulholland Helen , Kullu Cecil , Hann Mark , Duarte Rui , Mattocks Felicity , Guthrie Else , Gabbay Mark 
Published: August 2021

Abstract
Background
People who self-harm are at high risk for future suicide and often suffer considerable emotional distress. Depression is common among people who self-harm and may be an underlying driver of self-harm behaviour. Self-harm is often repeated, and risk of repetition is highest immediately after an act of self-harm. Readily accessible brief talking therapies show promise in helping people who self-harm, but further evaluation of these approaches is needed. A brief talking therapy intervention for depression and self-harm has been designed for use in a community setting. This mixed methods feasibility study with repeated measures will examine the feasibility and acceptability of the Community Outpatient Psychological Engagement Service for Self-Harm (COPESS) for people with self-harm and depression in the community, compared to routine care.

Methods
Sixty participants with a history of self-harm within the last six months, who are also currently depressed, will be recruited to take part in a feasibility single-blind randomised controlled trial (RCT). Participants will be randomised 1:1 to receive COPESS plus treatment as usual (TAU) or TAU alone. Recruitment will be via General Practitioners (GP) and self-referral. Assessment of feasibility and acceptability will be assessed via quantitative and qualitative methods including measures of recruitment and retention to the feasibility trial, participants’ experience of therapy, completion/completeness of outcome measures at relevant time-points and completion of a service use questionnaire.

Discussion
The results will indicate whether it is feasible to conduct a definitive full trial to determine whether COPESS is a clinically and cost effective intervention for people who self-harm in the community. Qualitative and quantitative data will in addition help identify potential strengths and/or challenges of implementing brief community-based interventions for people who self-harm.

Trial registration
NCT04191122 registered 9th December 2019.
Quantitative Systems Pharmacology Modeling of Avadomide-Induced Neutropenia Enables Virtual Clinical Dose and Schedule Finding Studies
Authors: Abbiati Roberto A. , Pourdehnad Michael , Carrancio Soraya , Pierce Daniel W. , Kasibhatla Shailaja , McConnell Mark , Trotter Matthew W. B. , Loos Remco , Santini Cristina C. , Ratushny Alexander V. 
Published: August 2021

AbstractAvadomide is a cereblon E3 ligase modulator and a potent antitumor and immunomodulatory agent. Avadomide trials are challenged by neutropenia as a major adverse event and a dose-limiting toxicity. Intermittent dosing schedules supported by preclinical data provide a strategy to reduce frequency and severity of neutropenia; however, the identification of optimal dosing schedules remains a clinical challenge. Quantitative systems pharmacology (QSP) modeling offers opportunities for virtual screening of efficacy and toxicity levels produced by alternative dose and schedule regimens, thereby supporting decision-making in translational drug development. We formulated a QSP model to capture the mechanism of avadomide-induced neutropenia, which involves cereblon-mediated degradation of transcription factor Ikaros, resulting in a maturation block of the neutrophil lineage. The neutropenia model was integrated with avadomide-specific pharmacokinetic and pharmacodynamic models to capture dose-dependent effects. Additionally, we generated a disease-specific virtual patient population to represent the variability in patient characteristics and response to treatment observed for a diffuse large B-cell lymphoma trial cohort. Model utility was demonstrated by simulating the avadomide effect in the virtual population for various dosing schedules and determining the incidence of high-grade neutropenia, its duration, and the probability of recovery to low-grade neutropenia.
ARL3 and ARL13B GTPases participate in distinct steps of INPP5E targeting to the ciliary membrane
Authors: Fujisawa Sayaka , Qiu Hantian , Nozaki Shohei , Chiba Shuhei , Katoh Yohei , Nakayama Kazuhisa 
Published: August 2021

ABSTRACT
INPP5E, a phosphoinositide 5-phosphatase, localizes on the ciliary membrane via its C-terminal prenyl moiety, and maintains the distinct ciliary phosphoinositide composition. The ARL3 GTPase contributes to the ciliary membrane localization of INPP5E by stimulating the release of PDE6D bound to prenylated INPP5E. Another GTPase, ARL13B, which is localized on the ciliary membrane, contributes to the ciliary membrane retention of INPP5E by directly binding to its ciliary targeting sequence. However, as ARL13B was shown to act as a guanine nucleotide exchange factor (GEF) for ARL3, it is also possible that ARL13B indirectly mediates the ciliary INPP5E localization via activating ARL3. We here show that INPP5E is delocalized from cilia in both ARL3-knockout (KO) and ARL13B-KO cells. However, some of the abnormal phenotypes were different between these KO cells, while others were found to be common, indicating the parallel roles of ARL3 and ARL13B, at least concerning some cellular functions. For several variants of ARL13B, their ability to interact with INPP5E, rather than their ability as an ARL3-GEF, was associated with whether they could rescue the ciliary localization of INPP5E in ARL13B-KO cells. These observations together indicate that ARL13B determines the ciliary localization of INPP5E, mainly by its direct binding to INPP5E.
Hypusinated eIF5A is required for the translation of collagen
Authors: Barba-Aliaga Marina , Mena Adriana , Espinoza Vanessa , Apostolova Nadezda , Costell Mercedes , Alepuz Paula 
Published: August 2021

ABSTRACT
Translation of mRNAs that encode peptide sequences with consecutive prolines (polyproline) requires the conserved and essential elongation factor eIF5A to facilitate the formation of peptide bonds. It has been shown that, upon eIF5A depletion, yeast ribosomes stall in polyproline motifs, but also in tripeptide sequences that combine proline with glycine and charged amino acids. Mammalian collagens are enriched in putative eIF5A-dependent Pro-Gly-containing tripeptides. Here, we show that depletion of active eIF5A in mouse fibroblasts reduced collagen type I α1 chain (Col1a1) content, which concentrated around the nuclei. Moreover, it provoked the upregulation of endoplasmic reticulum (ER) stress markers, suggesting retention of partially synthesized collagen 1 (Col1) in the ER. We confirmed that eIF5A is needed for heterologous collagen synthesis in yeast and, using a double luciferase reporter system, showed that eIF5A depletion interrupts translation at Pro-Gly collagenic motifs. A dramatically lower level of Col1a1 protein was also observed in functional eIF5A-depleted human hepatic stellate cells treated with the profibrotic cytokine TGF-β1. In sum, our results show that collagen expression requires eIF5A and imply its potential as a target for regulating collagen production in fibrotic diseases.
Python-Microscope – a new open-source Python library for the control of microscopes
Authors: Susano Pinto David Miguel , Phillips Mick A. , Hall Nicholas , Mateos-Langerak Julio , Stoychev Danail , Susano Pinto Tiago , Booth Martin J. , Davis Ilan , Dobbie Ian M. 
Published: August 2021

ABSTRACT
Custom-built microscopes often require control of multiple hardware devices and precise hardware coordination. It is also desirable to have a solution that is scalable to complex systems and that is translatable between components from different manufacturers. Here we report Python-Microscope, a free and open-source Python library for high-performance control of arbitrarily complex and scalable custom microscope systems. Python-Microscope offers simple to use Python-based tools, abstracting differences between physical devices by providing a defined interface for different device types. Concrete implementations are provided for a range of specific hardware, and a framework exists for further expansion. Python-Microscope supports the distribution of devices over multiple computers while maintaining synchronisation via highly precise hardware triggers. We discuss the architectural features of Python-Microscope that overcome the performance problems often raised against Python and demonstrate the different use cases that drove its design: integration with user-facing projects, namely the Microscope-Cockpit project; control of complex microscopes at high speed while using the Python programming language; and use as a microscope simulation tool for software development.
Supramolecular Polymers and Materials Formed by Host-Guest Interactions
Authors: Harada Akira , Takashima Yoshinori , Hashidzume Akihito , Yamaguchi Hiroyasu 
Published: August 2021

Abstract
Supramolecular polymers and materials have been designed and prepared using macromolecular recognition by cyclodextrins as host parts. Polyrotaxanes, tubular polymers, supramolecular machines, artificial polymerases have been obtained by main-chain recognition. Macroscopic-self-assembly, self-healing materials, sol-gel transition, muscle-like materials have been achieved by side-chain recognition. End-group recognition leads to the construction of supramolecular polymers in narrow sense.
Supramolecule Stripped MoS2 Nanosheets for Enhanced Surface Plasmon Resonance Spectroscopy Application
Authors: Chen Jie , Hu Ruhui , Zhu Han , Wang Yindian , Mao Zhihui , Hu JunJie , Hu Xiaojun , Chen Hongxia 
Published: August 2021

Abstract
Molybdenum disulfide nanosheets (MoS2 NSs) show great promise in the field of optical biosensors due to its unique optical transparency and semiconducting properties. In this work, supramolecule calix[4]arene (pSC4) modified MoS2 NSs (pSC4-MoS2 NSs) was synthesized through a one-step preparation method. The strong p-π binding and electrostatic interactions between pSC4 and MoS2 NSs increased the stripping efficiency by preventing aggregation of nanosheets. Based on pSC4-MoS2 NSs, a simple and feasible surface plasmon resonance (SPR) sensor was proposed. SPR refractive index change experiments were conducted to evaluate the sensor’s sensitivity performance, and the three main parameters of sensitivity were improved. Benefiting from the modification of pSC4, proteins were captured with higher surface coverage through the host-guest recognition, which is identical to optimization analysis experiment. According to a bovine serum albumin antibody immune experiment, pSC4-MoS2 NSs based SPR sensors provided a new sensitive sensing interface with a 50.85% SPR signal increased compared that of MoS2 NSs modification. Therefore, supramolecule modified MoS2 NSs is promising for application to SPR immunosensing for the detection of cancer biomarkers.
Materials Informatics for 2D Materials Combined with Sparse Modeling and Chemical Perspective: Toward Small-Data-Driven Chemistry and Materials Science
Authors: Oaki Yuya , Igarashi Yasuhiko 
Published: August 2021

Abstract
Application of data-scientific approaches to conventional sciences, such as chemo-informatics, bio-informatics, and materials informatics (MI), has attracted much interest toward data-driven research. The concept enables accelerated discovery of new materials, enhancement of performance, and optimization of processes. However, sufficient bigdata is not always prepared to apply to machine learning. For example, experimental scientists have their own small data including success and failure in their laboratory, whether in academia or industry. If such small data is effectively utilized with a data-scientific approach, research activities can be accelerated without energy, resource, and cost consumption. This account focuses on MI for small data, a recent concept for application of small data, with introduction of model cases, such as control of exfoliation processes to obtain 2D materials. Combination of machine learning and chemical perspective is effective for construction of straightforward and interpretable predictors through the extraction of a limited number of descriptors from small dataset. Although the prediction accuracy is not so precise, the model has enough accuracy to be a guideline reducing the number of the next experiments. The present MI for small data opens potentials of small-data-driven chemistry and materials science.