bluuebunny/crossref_metadata_2025_split huggingface dataset:
The influence of the SARS-CoV-2 lockdown on patients with inflammatory rheumatic diseases on their adherence to immunomodulatory medication: a cross sectional study over 3 months in Germany
Authors: Hasseli R , Müller-Ladner U , Keil F , Broll M , Dormann A , Fräbel C , Hermann W , Heinmüller C -J , Hoyer B F , Löffler F , Özden F , Pfeiffer U , Saech J , Schneidereit T , Schlesinger A , Schwarting A , Specker C , Stapfer G , Steinmüller M , Storck-Müller K , Strunk J , Thiele A , Triantafyllias K , Vagedes D , Wassenberg S , Wilden E , Zeglam S , Schmeiser T 
Published: March 2021

Abstract

Objectives
To evaluate the influence of the SARS-CoV-2 pandemic on the adherence of patients with inflammatory rheumatic diseases (IRD) to their immunomodulatory medication during the three-month lockdown in Germany.

Methods
From 16th March until 15th June 2020, IRD patients from private practices and rheumatology departments were asked to answer a questionnaire addressing their behaviour with respect to their immunomodulating therapy. Eight private practices and nine rheumatology departments that included rheumatology primary care centres and university hospitals participated. A total of 4252 questionnaires were collected and evaluated.

Results
The majority of patients (54%) were diagnosed with RA, followed by psoriatic arthritis (14%), ankylosing spondylitis (10%), connective tissue diseases (12%) and vasculitides (6%). Most of the patients (84%) reported to continue their immunomodulatory therapy. Termination of therapy was reported by only 3% of the patients. The results were independent from the type of IRD, the respective immunomodulatory therapy and by whom the patients were treated (private practices vs rheumatology departments). Younger patients (&lt;60 years) reported just as often as older patients to discontinue their therapy.

Conclusion
The data show that most of the patients continued their therapy in spite of the pandemic. A significant change in behaviour with regard to their immunomodulatory therapy was not observed during the three months of observation. The results support the idea that the immediate release of recommendations of the German Society of Rheumatology were well received, supporting the well-established physician–patient relationship in times of a crisis.
Religious Moral Communities or Secular Tolerance? Individual-, National-, and Regional-Level Explanations for Restrictive Refugee Policy Preferences in Europe
Authors: Vaughan Kenneth R 
Published: March 2021

AbstractReligious communities can encourage generosity toward strangers. In these communities, such norms are high among religious individuals and nonadherents. It remains unclear how these norms inform policy. Recent refugee crises provide critical tests of how charitable values translate into political preferences. As Europe experiences turmoil over the acceptance and integration of Muslim refugees, I ask how religion affects preferences regarding refugee admissions. I find that marginalized religious groups among new immigrants offer the highest levels of support for admitting refugees. Catholics are more restrictive than religious nones regarding policy preferences, while there are mixed results comparing nones to other Christian denominations. However, for Catholics and most other Christians, generous policy preferences increase with higher rates of religious service attendance. Respondents offer more generous policy preferences in regions with higher rates of Protestant and Catholic affiliation and in nations with higher Protestant affiliation. Restrictive preferences increase with regional and national percent unaffiliated.
A field trial comparing four oral nonsteroidal anti-inflammatory drugs on controlling cautery dehorning pain and stress in calves
Authors: Stock Matthew L , Kleinhenz Michael D , Mazloom Reza , Jaberi-Douraki Majid , Barth Laura A , Van Engen Nicholas K , Voris Erica A , Wang Chong , Coetzee Johann F 
Published: March 2021

Abstract
The purpose of this study was to compare the analgesic effect of four nonsteroidal anti-inflammatory drugs (NSAIDs) administered as a single, standardized, oral dose in dairy calves at the time of cautery dehorning. The NSAIDs investigated have pharmacokinetic properties in cattle that produce persistent plasma concentrations that may provide prolonged analgesia with the added practicality of a simple administration regimen. One hundred and eighty-five Holstein calves aged approximately 50 d old were either sham dehorned (n = 31) or cautery dehorned following oral administration of carprofen (n = 31), firocoxib (n = 31), flunixin meglumine (n = 30), meloxicam (n = 31) or placebo (n = 31) in a randomized, controlled trial. A standard dose of 2.0 mg/kg was administered to all calves receiving an oral NSAID. All calves received local anesthesia prior to actual or sham dehorning. Cortisol concentrations, heart rate, mechanical nociception thresholds, ocular and dehorning area temperatures, and average daily gains were evaluated. A linear mixed-effects model with repeated measures was used for statistical analysis. Administration of oral meloxicam, flunixin meglumine, and firocoxib at 2.0 mg/kg resulted in decreased cortisol concentrations compared to placebo-treated controls for the first 24 h postdehorning (AUEC0–24) (P = 0.03). Moreover, firocoxib, flunixin meglumine, and meloxicam attenuated the maximum cortisol concentrations compared to placebo-treated calves (P = 0.04, P= 0.02). In calves treated with flunixin meglumine, cortisol concentrations was reduced at 4 h (P = 0.04) and 8 h (P = 0.02). In addition, analgesic administration was associated with changes in ocular and dehorning area temperature differences (P = 0.09). Carprofen and meloxicam reduced heart rates during the entire study period (P = 0.003). Although a treatment effect (P &lt; 0.0001) was observed in the determination of mechanical nociception threshold among all treatment groups, meloxicam expressed marginally significant effects (P = 0.09) among NSAID treated groups dehorned. A single dose of oral meloxicam, flunixin meglumine, or firocoxib administered at 2.0 mg/kg reduced the acute stress response associated with cautery dehorning. However, carprofen administration was associated with increased cortisol concentrations and dehorning area temperatures for the initial 24 h. Given the changes in pain and stress outcome variables assessed in this study, NSAIDs should be administered at the time of dehorning.
High-Frequency Visual Stimulation Primes Gamma Oscillations for Visually Evoked Phase Reset and Enhances Spatial Acuity
Authors: Lantz Crystal L , Quinlan Elizabeth M 
Published: March 2021

Abstract
The temporal frequency of sensory stimulation is a decisive factor in the plasticity of perceptual detection thresholds. However, surprisingly little is known about how distinct temporal parameters of sensory input differentially recruit activity of neuronal circuits in sensory cortices. Here we demonstrate that brief repetitive visual stimulation induces long-term plasticity of visual responses revealed 24 h after stimulation and that the location and generalization of visual response plasticity is determined by the temporal frequency of the visual stimulation. Brief repetitive low-frequency stimulation (2 Hz) is sufficient to induce a visual response potentiation that is expressed exclusively in visual cortex layer 4 and in response to a familiar stimulus. In contrast, brief, repetitive high-frequency stimulation (HFS, 20 Hz) is sufficient to induce a visual response potentiation that is expressed in all cortical layers and transfers to novel stimuli. HFS induces a long-term suppression of the activity of fast-spiking interneurons and primes ongoing gamma oscillatory rhythms for phase reset by subsequent visual stimulation. This novel form of generalized visual response enhancement induced by HFS is paralleled by an increase in visual acuity, measured as improved performance in a visual detection task.
Pavement markings: identification of relevant covariates and controllable factors of retroreflectivity performance as a road safety measure
Authors: Lee Ho Linda , Bueno Filho Júlio Silvio de Souza , Fujii Walquiria Yumiko , Machado Cláudia A S , Bernucci Liedi Legi Bariani , Quintanilha José Alberto 
Published: March 2021

Abstract
In this paper we present relevant contributions and important features related to the study of the retroreflectivity performance of pavement markings. The contribution of this paper is threefold. First, we propose an artificial scheme to allow some randomization of the treatments owing to several restrictions imposed on the choice of the experimental units. It is an experiment involving one fixed factor (three types of materials) in a randomized block design executed on a high-traffic-volume highway. Under this condition, the traffic volume works as a stress factor and the degradation of the retroreflectivity of pavement markings is faster than the degradation on rural roads or streets. This is related to the second contribution: the possibility of a reduction of experimental time. The current experiment spent 20 weeks to collect the data. And finally a mixed linear model considering three random effects and several fixed effects is fitted and the most relevant effects pointed out. This study can help highway managers to improve road safety by scheduling the maintenance of pavement marks at the appropriate time, choosing adequate material for the pavement markings and applying the proposed artificial scheme in future studies.
Risk of hospitalization and risk of death for healthcare workers with COVID-19 in nine European Union/European Economic Area countries, January 2020–January 2021
Authors: Ferland Lisa , Dias Joana Gomes , Carvalho Carlos , Adlhoch Cornelia , Suetens Carl , Beauté Julien , Kinross Pete , Plachouras Diamantis , Lamb Favelle , Hannila-Handelberg Tuula , Fabiani Massimo , Riccardo Flavia , Mossong Joël , Vergison Anne , van Gageldonk-Lafeber Rianne , Teirlinck Anne , Melillo Jackie , Melillo Tanya , Mook Piers , Pebody Richard , Rehse Ana Paula Coutinho , Monnet Dominique L. 
Published: March 2021

AbstractWe assessed the impact of COVID-19 on healthcare workers (HCWs) from data on 2.9 million cases reported from nine countries in the EU/EEA. Compared to non-HCWs, HCWs had a higher adjusted risk of hospitalization (IRR 3.0 [95% CI 2.2-4.0]), but not death (IRR 0.9, 95% CI 0.4-2.0).Article Summary LineHealthcare workers are hospitalized more frequently than non-healthcare workers when adjusting for age, sex, and comorbidities.
Direct detection of microorganisms responsible for the sexually transmitted infection on the mobile real-time PCR device without treating in advance
Authors: Muraoka Masaaki , Sohma Kazunori , Kawaguchi Osamu , Mizukoshi Mikio 
Published: March 2021

ABSTRACTAs WHO reported, four curable STIs-chlamydia, gonorrhoea, syphilis and trichomoniasis occur more than 1 million per each day globally almond 2016. For this reason, it is important to control these STIs, one of which is “to detect”. The general methods in order to detect STIs are nucleic acid amplification tests (NAATs). One of the reasons why NAATs are utilized in many tests is that it is possibly to be more sensitive than other test. However, there needs to treat extraction of nucleic acids in advance and amplify specific regions by NAATs, and hence it must take much labour and much time. In this work, for Chlamydia trachomatis (CT), Neisseria gonorrhoeae (NG) and Treponema pallidum (TP) which is each etiological agent of chlamydia, gonorrhoea and syphilis, we evaluate and propose “quicker and simpler” NAATs. Specifically, utilizing mobile real-time PCR device “PCR1100” and PCR reagent kit “KAPA3G Plant PCR Kit”, it was considered whether real-time direct PCR could be performed or not without treating DNA extraction in advance so-called “direct”.As a result, firstly, we established that real-time direct PCR could be performed in all of CT, NG, and TP, and moreover, each Ct value correlated with the concentration of each organism similarly to detection of genome DNA (each correlation coefficient R2 > 0.95). Moreover, each assay demonstrated a limit of detection (LOD) of the follows; CT was 10^0.86 = 7.24 IFU/reaction, NG was 10^-0.19 = 0.65 CFU/reaction, and TP was 10^1.4 = 25.1 organisms/reaction. However, it appeared the sensitivity was a little low, especially for CT and TP.Secondly, we found that even as without treating sample in advance, the time of detection was required more less 15 minutes at any of case, which was very quick compared with other current methods for real-time PCR. Additionally, compared with other commercial devices, it was easier to operate the PCR1100 device, for example, start, analysis of Ct value.In conclusion, the present study has demonstrated that it is possible for real-time direct PCR to perform with combination of the PCR1100 device and the PCR reagent kit in 3 kinds of microorganisms-CT, NG and TP. Furthermore, we propose “quicker and simpler” methods for NAATs, which it would not take labour and time. Further studies are needed in order to contribute to control STIs.
Timing of parental depression on risk of child depression and poor educational outcomes: a population based routine data cohort study from Born in Wales, UK
Authors: Brophy Sinead , Todd Charlotte , Rahman Muhammad A , Kennedy Natasha , Rice Frances 
Published: March 2021

AbstractBackgroundMaternal depression is a risk factor for depression in children, though the influence of paternal depression has been less well examined. We examined the association between maternal and paternal depression, and the timing of their depression (before or after the child’s birth) and outcomes for the child including incidence of child depression and poor educational attainment.MethodsA linked routine data cohort study linking General Practitioner(GP), hospital and education records of young people (aged 0 to 30 years) in Wales. Parental and child diagnosis of depression was identified from GP data. Regression analysis examined the association of maternal and paternal depression with time to diagnosis of depression in the child and odds of attaining educational milestones.OutcomesIn adjusted models, the relative risk of offspring developing depression was 1.22 if the mother had depression before the child was born, 1.55 if the mother had depression after the child was born and 1.73 if she had depression both before and after the child was born (chronic depression), compared to those were there was no maternal depression history. For achieving milestones at end of primary school, odds were 0.92, 0.88 and 0.79 respectively. Association of depression in the child was similar if the male living in the household had depression with risk ratios of 1.24 (before), 1.43 (after) and 1.27 (before and after) for child diagnosed depression and 0.85, 0.79 and 0.74 for achieving age 11 milestones.InterpretationChildren who live with a parent who has depression are more likely to develop depression and not achieve educational milestones, compared to children who live with a parent who has a history of depression (but no active depression in child’s lifetime) and compared to those with no depression. This finding suggests that working closely with families where depression (particularly chronic depression) is present in either parent and treating parental depression to remission is likely to have long-term benefits for children’s mental health and educational attainment.FundingThis study had no specific funding. The infrastructure to enable the study was funded by Health Care Research Wales (https://healthandcareresearchwales.org/) which funded; the National Centre for Population Health and Wellbeing Research (https://ncphwr.org.uk/) enabling the involvement of SB, CT, MAR, TK, the National Centre for Mental Health Wales (https://www.ncmh.info/), which supported the involvement of FR, and the Secure Anonymised Information Linkage Database (https://saildatabank.com/)
CNBP, REL, and BHLHE40 variants are associated with IL-12 and IL-10 responses and tuberculosis risk
Authors: Shah Javeed A. , Warr Alex J. , Graustein Andrew D. , Saha Aparajita , Dunstan Sarah J. , Thuong Nguyen T.T. , Thwaites Guy E. , Caws Maxine , Thai Phan V.K. , Bang Nguyen D. , Chau Tran T.H. , Nguyen Felicia K. , Hernandez Carlo A. , Jones Madison A. , Sassetti Christopher M. , Fitzgerald Katherine A. , Musvosvi Munyaradzi , Gela Anele , Hanekom Willem A. , Hatherill Mark , Scriba Thomas J. , Hawn Thomas R. 
Published: March 2021

AbstractRationaleThe major human genes regulating M. tuberculosis (Mtb)-induced immune responses and tuberculosis (TB) susceptibility are poorly understood. Although IL-12 and IL-10 are critical for TB pathogenesis, the genetic factors that regulate their expression are unknown. CNBP, REL, and BHLHE40 are master regulators of IL-12 and IL-10 signaling.ObjectivesTo determine whether common human genetic variation in CNBP, REL and BHLHE40 is associated with IL-12 and IL-10 expression, adaptive immune responses to mycobacteria, and susceptibility to TB.Methods and Main MeasurementsWe characterized the association between common variants in CNBP, REL, and BHLHE40 and innate immune responses in dendritic cells and monocyte-derived macrophages (MDM), BCG-specific T cell responses, and susceptibility to pediatric and adult TB.ResultsSNP BHLHE40 rs4496464 was associated with increased BHLHE40 expression in MDMs and increased IL-10 from both peripheral blood dendritic cells and MDMs after LPS and TB whole cell lysate stimulation. SNP BHLHE40 rs11130215, in linkage disequilibrium with rs4496464, was associated with increased BCG-specific IL2+CD4+ T cell responses and decreased risk for pediatric TB in South Africa. SNPs REL rs842634 and CNBP rs11709852 were associated with increased IL-12 production from dendritic cells, and SNP REL rs842618, in linkage disequilibrium with rs842634, was associated with increased risk for TB meningitis.ConclusionsGenetic variation in CNBP, REL, and BHLHE40 is associated with IL-12 and IL-10 cytokine response and TB clinical outcomes. Common human genetic regulation of well-defined intermediate cellular traits provides insights into mechanisms of TB pathogenesis.
Knowledge transfer to enhance the performance of deep learning models for automated classification of B-cell neoplasms
Authors: Mallesh Nanditha , Zhao Max , Meintker Lisa , Höllein Alexander , Elsner Franz , Lüling Hannes , Haferlach Torsten , Kern Wolfgang , Westermann Jörg , Brossart Peter , Krause Stefan W. , Krawitz Peter M. 
Published: March 2021

AbstractMulti-parameter flow cytometry (MFC) is a cornerstone in clinical decision making for hematological disorders such as leukemia or lymphoma. MFC data analysis requires trained experts to manually gate cell populations of interest, which is time-consuming and subjective. Manual gating is often limited to a two-dimensional space. In recent years, deep learning models have been developed to analyze the data in high-dimensional space and are highly accurate. Such models have been used successfully in histology, cytopathology, image flow cytometry, and conventional MFC analysis. However, current AI models used for subtype classification based on MFC data are limited to the antibody (flow cytometry) panel they were trained on. Thus, a key challenge in deploying AI models into routine diagnostics is the robustness and adaptability of such models. In this study, we present a workflow to extend our previous model to four additional MFC panels. We employ knowledge transfer to adapt the model to smaller data sets. We trained models for each of the data sets by transferring the features learned from our base model. With our workflow, we could increase the model’s overall performance and more prominently, increase the learning rate for very small training sizes.
Cost-Sharing in Medical Care Can Increase Adult Mortality Risk in Lower-Income Countries
Authors: Buitrago Giancarlo , Miller Grant , Vera-Hernández Marcos 
Published: March 2021

AbstractPatient cost-sharing in medical care constrains total health spending, presumably with little harm to underlying patient health. This paper re-evaluates the link between cost-sharing and health, studying Colombia’s entire formal sector workforce with individual-level health care utilization records linked to payroll data and vital statistics. Given discrete breaks in outpatient cost-sharing imposed at multiple income thresholds by Colombia’s national health system, we use a regression discontinuity design and find that outpatient cost-sharing reduces use of outpatient care, resulting in fewer diagnoses of common chronic diseases and increasing subsequent emergency room visits and hospitalizations. Ultimately, these effects measurably increase mortality, and disproportionately so among the poor – raising the absolute difference in 7-year mortality risk by 0.80 and 0.23 deaths per 1,000 individuals at lower- and higher-income thresholds, respectively. To the best of our knowledge, this study is the first to show a relationship between cost-sharing and adult mortality risk in lower-income countries, a relationship important to incorporate into social welfare analyses of cost-sharing policies.One Sentence SummaryOutpatient cost-sharing in medical care discourages use – but over time, also increases costly hospital service use and raises mortality risk.
The COVID-19 health equity twindemic: Statewide epidemiologic trends of SARS-CoV-2 outcomes among racial minorities and in rural America
Authors: Dixon Brian E. , Grannis Shaun J. , Lembcke Lauren , Roberts Anna , Embi Peter J. 
Published: March 2021

AbstractBackgroundEarly studies on COVID-19 identified unequal patterns in hospitalization and mortality in urban environments for racial and ethnic minorities. These studies were primarily single center observational studies conducted within the first few weeks or months of the pandemic. We sought to examine trends in COVID-19 morbidity and mortality over time for minority and rural populations, especially during the U.S. fall surge.MethodsStatewide cohort of all adult residents in Indiana tested for SARS-CoV-2 infection between March 1 and December 31, 2020, linked to electronic health records. Primary measures were per capita rates of infection, hospitalization, and death. Age adjusted rates were calculated for multiple time periods corresponding to public health mitigation efforts.ResultsMorbidity and mortality increased over time with notable differences among sub-populations. Initially, per capita hospitalizations among racial minorities were 3-4 times higher than whites, and per capita deaths among urban residents were twice those of rural residents. By fall 2020, per capita hospitalizations and deaths in rural areas surpassed those of urban areas, and gaps between black/brown and white populations narrowed. Cumulative morbidity and mortality were highest among minority groups and in rural communities.ConclusionsBurden of COVID-19 morbidity and mortality shifted over time, creating a twindemic involving disparities in outcomes based on race and geography. Health officials should explicitly measure disparities and adjust mitigation and vaccination strategies to protect vulnerable sub-populations with greater disease burden.