bluuebunny/crossref_metadata_2025_split huggingface dataset:
Preparation of red and grey elemental selenium for food fortification
Authors: Khandsuren B. , Prokisch J. 
Published: April 2021

AbstractIn recent years, the importance of nanomaterials in food science, medicine, etc. has been increasing quickly. Herein, organic and inorganic red selenium nanoparticles synthesised by the reduction of sodium selenite with chemical and biological reducing agents. Grey hexagonal form in aqueous and powder was assembled at a high temperature of 85 °C for 10 min. Also, selenium enriched yogurt powder was made that contained about 2,000 mg kg−1 selenium, 93.8% of which is in nano form with a size of 50–500 nm. The synthesised nanoparticles were characterised by Dynamic Light Scattering Particle Size Analyzer (DLS), X-ray Diffraction Analysis (XRD), Scanning Electron Microscope (SEM) and Transmission Electron Microscope (TEM). The prepared SeNPs could be promising additive for a wide range of applications.
Measures for flood discharge transformation on the Ondava River
Authors: Mydla Jakub , Šoltész Andrej , Orfánus Martin 
Published: April 2021

AbstractThe contribution is dealing with run-off conditions on the lower part of the Ondava River as the capacity of the river bed is insufficient at high flow rates. The reason for the proposed research was the flood situations when protection dikes were breached. For mathematical modeling of flood wave progress, the HEC-RAS software has been applied coupling 1D and 2D modeling procedures. Results of the mathematical model of the surface water level regime in the Ondava River were compared with measured values and afterwards it was used to design further flood protection measures utilizing the existing drainage channel system and pumping stations, as well.
Thermal performance of inserting hybrid nanofluid in parabolic trough collector
Authors: Al-Oran Otabeh , Lezsovits Ferenc 
Published: April 2021

Abstract
In this work, the thermal performance of using hybrid nanofluid of Ceria oxide and multi-walled carbon nanotube-based MOL 68 in the receiver tube of parabolic trough collector is simulated numerically. The influence of using this nanofluid under various volume concentrations and different Reynold numbers is solved numerically using computational fluid dynamics. The turbulent model's analysis is carried out based on k–ϵ re-normalization group and employed to find the Nusselt number and the heat transfer coefficients. The model results were validated with the previous correlation, which were used to evaluate the Nusselt number. The results showed that hybrid nanofluid enhances the heat transfer characteristics of the parabolic trough collector in comparison with the base fluid. Furthermore, even better heat transfer characteristics can be achieved with an increased volume concentration of the modified nanofluids.
Error sources and models of the laser tracker without a beam steering mirror
Authors: Choleva Radoslav , Kopáčik Alojz 
Published: April 2021

Abstract
The laser tracker is a widely used instrument in many industrial and metrological applications with high demand measurement accuracy. Imperfections in construction and misalignment of individual parts deliver systematic errors in the measurement results. All error sources need to be identified and reduced to the minimum to achieve the best possible accuracy. The paper summarizes error sources of the laser tracker without beam steering mirror with emphasis on error modeling. Descriptions of error models are provided for the static and kinematic type of measurement.
Immunolocalization of Protein Kinase C Isoenzymes α, βI and βII in Rat Kidney
Authors: PFAFF IMKE L. , WAGNER HANS-JOACHIM , VALLON VOLKER 
Published: April 2021

Abstract.
Protein kinase C (PKC) significantly contributes to the control of renal function, but little is known about the renal function or localization of PKC isoenzymes. Therefore, the localization of PKC isoenzymes α, βI, and βII was studied in rat kidney. Immunoblot analysis identified immunoreactive bands corresponding to PKC α, βI, and βII in total cell extracts of both renal cortex and medulla. Immunohistochemistry using confocal laser scanning microscopy revealed immunostaining for PKC α within the glomerulus including podocytes and mesangial cells. PKC βI was detected in mesangial cells, whereas anti-PKC βII labeled neither podocytes nor mesangial cells. PKC βII, however, was detected in cells within the mesangial area, which expressed MHC II, a marker for antigen-presenting cells. None of the three isoforms was detected in glomerular endothelial cells. A prominent immunostaining with anti-PKC α and βI was localized to the brush border of S2 and S3 segments of proximal tubule, whereas S1 segments were not stained. Along the loop of Henle, both PKC α and PKC βI were found in the luminal membrane of cortical and medullary thick ascending limb. In addition, anti-PKC βI labeled the luminal membrane of thin limbs. In the cortical collecting duct (CCD), immunofluorescence for PKC α was observed at the apical membrane of both peanut agglutinin (PNA)-negative cells and part of PNA-positive cells, whereas in the medullary collecting duct (MCD), PKC α was detected at the basolateral membrane. In comparison, PKC βI was localized at the luminal membrane of PNA-positive cells only in CCD and at the luminal membrane of MCD. Unlike PKC α or βI, there was (1) no detectable immunostaining with anti-PKC βII in the proximal tubule, the loop of Henle, or the CCD and (2) a distinct staining for PKC βII of interstitial cells in cortex and medulla (including MHC II-positive dendritic cells). Furthermore, PKC βII was detected in the luminal membrane of MCD. In summary, a distinct and differential expression pattern for PKC α, βI, and βII was shown in rat kidney, which may contribute to a better understanding of the specific role of these isoenzymes in the control of renal function.
High Glucose Stimulates Proliferation and Collagen Type I Synthesis in Renal Cortical Fibroblasts
Authors: HAN DONG CHEOL , ISONO MOTOHIDE , HOFFMAN BRENDA B. , ZIYADEH FUAD N. 
Published: April 2021

Abstract.
Renal tubular epithelial cells and interstitial fibroblasts are active participants in tubulointerstitial fibrosis, the best correlate of decreased glomerular filtration in diabetic nephropathy. It was reported previously that high ambient glucose stimulates transforming growth factor-β (TGF-β) mRNA and bioactivity, promotes cellular hypertrophy, and increases collagen synthesis in proximal tubular cells. This study evaluates the effects of high glucose and TGF-β on the behavior of murine renal cortical fibroblasts (TFB) in culture. High glucose (450 mg/dl) significantly increased [3H]-thymidine incorporation (by 60 to 80% after 24 to 72 h) and cell number, without significantly increasing cell death when compared with normal glucose (100 mg/dl). There also was a transient increase in the mRNA of the c-myc and egr-1 early-response genes. Exogenous TGF-β1 was promitogenic rather than antiproliferative in contrast to other renal cell types. Northern blot analysis demonstrated constitutive expression of TGF-β1, -β2, and -β3 transcripts. Exposure to high glucose increased all three TGF-β isoforms in a time-dependent manner. High glucose as well as exogenous TGF-β1 also increased [3H]-proline incorporation, α2(I) collagen mRNA, and type I collagen protein (measured by immunoassay). Treatment with a neutralizing pan-selective monoclonal anti-TGF-β antibody markedly attenuated the stimulation by high ambient glucose of thymidine incorporation, TGF-β1 mRNA, and type I collagen mRNA and protein levels. It is concluded that high ambient glucose and exogenous TGF-β1 share similar actions on renal fibroblasts. Moreover, the stimulation of cell proliferation and collagen type I synthesis in these cells by high ambient glucose are mediated by activation of an autocrine TGF-β system.
Role of Caspases in Hypoxia-Induced Necrosis of Rat Renal Proximal Tubules
Authors: EDELSTEIN CHARLES L. , SHI YUEXIAN , SCHRIER ROBERT W. 
Published: April 2021

Abstract.
The role of the caspases, a newly discovered group of cysteine proteases, was investigated in a model of hypoxia-induced necrotic injury of rat renal proximal tubules. An assay for caspases in freshly isolated rat proximal tubules was developed. There was a 40% increase in tubular caspase activity after 15 min of hypoxia in association with increased cell membrane damage as indicated by a threefold increase in lactate dehydrogenase release. The specific caspase inhibitor Z-Asp-2,6-dichlorobenzoyloxymethylketone (Z-D-DCB) attenuated the increase in caspase activity during 15 min of hypoxia and markedly decreased lactate dehydrogenase release in a dose-dependent manner. In the proximal tubules, Z-D-DCB also inhibited the hypoxia-induced increase in calpain activity, another cysteine protease. In contrast, when Z-D-DCB was added to purified calpain in vitro, there was no inhibition of calpain activity. The calpain inhibitor (2)-3-(4-iodophenyl)-2-mercapto-2-propenoic acid (PD150606) also inhibited the hypoxia-induced increase in caspase activity in proximal tubules, but did not inhibit the activity of purified caspase 1 in vitro. In these experiments, caspase activity was detected with the fluorescence substrate Ac-Tyr-Val-Ala-Asp-7-amido-4-methyl coumarin (Ac-YVAD-AMC), which is preferentially cleaved by caspase 1. However, minimal caspase activity was detected with the fluorescence substrate Ac-Asp-Glu-Val-Asp-7-amido-4-methyl coumarin (Ac-DEVD-AMC), which is cleaved by caspases 2, 3, and 7. The present study in proximal tubules demonstrates that (1) caspase inhibition protects against necrotic injury by inhibition of hypoxia-induced caspase activity; and (2) caspase 1 may be the caspase involved. Thus, although the role of caspases in apoptotic cell death is well established, this study provides new evidence that caspases contribute to necrotic cell death as well.
Clinical Presentation and Follow-Up of 30 Patients with Congenital Nephrogenic Diabetes Insipidus
Authors: VAN LIEBURG ANGENITA F. , KNOERS NINE V. A. M. , MONNENS LEO A. H. 
Published: April 2021

Abstract.
Congenital nephrogenic diabetes insipidus is characterized by insensitivity of the distal nephron to arginine vasopressin. Clinical knowledge of this disease is based largely on case reports. For this study, data were collected on clinical presentation and during long-term follow-up of 30 male patients with congenital nephrogenic diabetes insipidus. The majority of patients (87%) were diagnosed within the first 2.5 yr of life. Main symptoms at clinical presentation were vomiting and anorexia, failure to thrive, fever, and constipation. Three older patients were diagnosed as a result of events not directly related to the disease. Except for a possibly milder phenotype in patients with a G185C mutation, no clear relationship between clinical and genetic data could be found. Most patients were on hydrochlorothiazide-amiloride treatment without significant side effects. Two patients suffered from severe hydronephrosis with a small rupture of the urinary tract after a minor trauma, and two patients experienced episodes of acute urine retention. Height SD scores for age remained below the 50th percentile in the majority of patients, whereas weight for height SD scores showed a catch-up after several years of underweight.
Mycophenolate Mofetil for Maintenance Therapy of Wegener's Granulomatosis and Microscopic Polyangiitis
Authors: NOWACK RAINER , GÖBEL URSULA , KLOOKER PIETER , HERGESELL OLAF , ANDRASSY KONRAD , VAN DER WOUDE FOKKO J. 
Published: April 2021

Abstract.
Successful maintenance therapy with mycophenolate mofetil (MMF) 2 g/d and low-dose oral corticosteroids (OCS) over a period of 15 mo was given to patients with Wegener's granulomatosis (WG) (n = 9) and microscopic polyangiitis (MPA) (n = 2). All patients had severe generalized disease with pauci-immune necrotizing glomerulonephritis and received standard induction therapy with oral cyclophosphamide and OCS for a mean of 14 wk until remission was achieved. Of 11 patients, only one WG patient relapsed in the 14th month of maintenance therapy. Maintenance therapy with MMF was able to further reduce grumbling disease activity as measured by the Birmingham vasculitis activity score (BVAS2) and proteinuria that were still present by the end of induction therapy. OCS could be reduced to a median daily dose of 5 mg and discontinued in three patients. Possible drug-related adverse effects were transient and included abdominal pain, respiratory infection, diarrhea, leukopenia, and a cytomegalovirus-colitis in one patient that was successfully treated with ganciclovir. It is concluded that MMF in combination with low-dose OCS is well tolerated and effective for maintenance therapy of WG and MPA. Long-term treatment with MMF in these diseases is attractive because of its low toxicity. MMF will have to be studied further and compared with cyclophosphamide or azathioprine maintenance therapy in randomized trials.
Feedback Control of Glomerular Vascular Tone in Neuronal Nitric Oxide Synthase Knockout Mice
Authors: VALLON VOLKER , TRAYNOR TIMOTHY , BARAJAS LUCIANO , HUANG YUNING G. , BRIGGS JOSIE P. , SCHNERMANN JU[Combining Diaeresis]RGEN 
Published: April 2021

Abstract.
For further elucidation of the role of neuronal nitric oxide synthase (nNOS) in macula densa (MD) cells, experiments were performed in anesthetized nNOS knockout mice (nNOS -/-). At comparable levels of arterial BP, renal blood flow was not significantly different between nNOS +/+ and nNOS -/- (1.7 ± 0.2 versus 1.4 ± 0.1 ml/min), and autoregulation of renal blood flow was maintained to a pressure level of approximately 85 mmHg in both groups of mice (n = 6 in each group). The fall in proximal tubular stop-flow pressure in response to an increase in loop of Henle perfusion rate from 0 to 30 nl/min was comparable in nNOS +/+ and -/- mice (40.7 ± 1.6 to 32 ± 2 mmHg versus 40.6 ± 1.6 to 31.6 ± 2 mmHg; not significant; n = 13 versus 18 nephrons). Luminal application of the nonselective NOS inhibitor nitro-L-arginine (10-3 and 10-2 M) enhanced the perfusion-dependent fall in stop-flow pressure in nNOS +/+ (7 ± 1 to 13 ± 2 mmHg; P < 0.05) but not in nNOS -/- (7 ± 1 to 8 ± 1 mmHg; not significant) mice. nNOS -/- mice exhibited a lower nephron filtration rate, compared with nNOS +/+, during free-flow collections from early distal tubules (influence of MD intact, 7 ± 0.7 versus 10.9 ± 1 nl/min; P = 0.002) but not from late proximal tubule (influence of MD minimized, 10.1 ± 1 versus 11.7 ± 1 nl/min; not significant; n = 16 nephrons). Distal Cl concentration and fractional absorption of fluid or chloride up to the early distal tubule was not different between nNOS -/- and +/+ mice. The data indicate that nNOS in MD tonically attenuates the GFR-lowering influence of ambient luminal NaCl, which may serve to increase the fluid and electrolyte load to the distal tubule, consistent with a role of MD nNOS in tubuloglomerular feedback resetting.
Effects of Ammonia on Acid-Base Transport by the B-Type Intercalated Cell
Authors: FRANK AMY E. , WEINER I. DAVID 
Published: April 2021

Abstract.
Ammonia, in addition to its role as a constituent of urinary net acid excretion, stimulates cortical collecting duct (CCD) net bicarbonate reabsorption. The current study sought to begin determining the cellular transport processes through which ammonia regulates bicarbonate reabsorption by testing whether ammonia stimulates B-type intercalated cell bicarbonate secretion, bicarbonate reabsorption, or both. The effects of ammonia on single CCD intercalated cells was studied by use of measurements of intracellular pH taken from in vitro microperfused CCD segments after luminal loading of the pH-sensitive fluorescent dye BCECF. These results showed, first, that ammonia inhibited B-cell unidirectional bicarbonate secretion and that this occurred despite no effect of ammonia on apical Cl-/HCO3
- exchange activity. Second, ammonia increased the contribution of a SCH28080-sensitive apical H+-K+-ATPase to basal intracellular pH regulation and it stimulated basolateral Cl-/HCO3
- exchange activity. Thus, ammonia activated both apical proton secretion and basolateral base exit, consistent with stimulation of unidirectional bicarbonate reabsorption. It was concluded that ammonia regulates CCD net bicarbonate reabsorption, at least in part, through the coordinated regulation of the separate processes of B-cell bicarbonate reabsorption and bicarbonate secretion. These effects do not reflect a general activation of ion transport but, instead, reflect coordinated and specific regulation of ion transport.
Human Immunodeficiency Virus-1 Induces Loss of Contact Inhibition in Podocytes
Authors: SCHWARTZ ELISSA J. , CARA ANDREA , SNOECK HANS , ROSS MICHAEL D. , SUNAMOTO MASAAKI , REISER JOCHEN , MUNDEL PETER , KLOTMAN PAUL E. 
Published: April 2021

Abstract.
Human immunodeficiency virus—associated nephropathy (HIVAN) affects up to 10% of HIV-positive black adults and children and is the leading cause of renal disease in infected individuals. The disease is characterized by proliferation of renal epithelial cells, both glomerular and tubular. Diseased kidneys are enlarged, and glomerular visceral epithelial cells (podocytes) express proliferation markers. In a transgenic murine model of HIVAN expressing a deletion construct of HIV-1, the identical pathologic features are observed. It was demonstrated that HIV-1 mRNA is expressed in renal epithelium of the transgenic mouse and in patients with HIVAN, suggesting a direct role for HIV-1 in disease pathogenesis in both humans and the murine model. For investigating the mechanisms responsible for proliferative changes in podocytes, the HIV-1 transgenic mouse was bred onto the immortomouse background, and conditionally immortalized transgenic and nontransgenic podocyte cell lines were established. Transgenic podocytes demonstrated increased spontaneous proliferation, compared with nontransgenic podocytes at confluence, and they were found to have a greater percentage of cells in the proliferative phase of the cell cycle. It is striking that transgenic podocytes were not contact inhibited and formed aggregates in soft agar. Aggregates also formed when nontransgenic podocytes were infected with the identical HIV-1 construct used to generate the transgenic model. This demonstrates that the loss of contact inhibition is due to a direct effect of HIV-1. Therefore, proliferation induced by HIV-1 gene expression is likely to play a key role in the pathogenesis of HIVAN.
Angiotensin II and Prostaglandin Interactions on Systemic and Renal Effects of L-NAME in Humans
Authors: PERINOTTO PATRIZIA , BIGGI ALMERINA , CARRA NICOLETTA , ORRICO ANTONELLA , VALMADRE GIUSEPPE , DALL'AGLIO PIERPAOLO , NOVARINI ALMERICO , MONTANARI ALBERTO 
Published: April 2021

Abstract.
For investigation of whether interactions between prostaglandins and angiotensin II modulate renal response to acute nitric oxide synthesis inhibition in humans, seven young volunteers who were kept on a 240-mM Na diet underwent four experiments with 90 min of infusion of 3.0 μg/kg.min-1 NG-nitro-L-arginine methyl ester (L-NAME), each preceded by a 3-d treatment with placebo (PL), 50 mg of losartan (LOS), 75 to 125 mg of indomethacin (IND), or both drugs. Mean arterial pressure (MAP), GFR, effective renal plasma flow (ERPF), and Na excretion rate (UNaV) were measured at baseline and from 0 to 45 min and 45 to 90 min of L-NAME infusion. After PL, L-NAME reduced GFR by 5% at 45 min (P < 0.05) and by 9% at 90 min (P < 0.001), ERPF by 11 to 17% (P < 0.001), and UNaV by 28 to 45% (P < 0.001). MAP, unchanged at 45 min, rose by 5% (P < 0.001) at 90 min. LOS prevented pressor but not renal effects of L-NAME. With L-NAME+IND, MAP rose even at 45 min (+5%; P < 0.001 versus baseline) with a 10% rise at 90 min (P < 0.001). Changes in GFR (-13 to -20%), ERPF (-19 to -26%), and UNaV (-51 to -70%) were greater than those with L-NAME+PL or L-NAME+LOS (P < 0.05 to 0.001). With L-NAME+IND+LOS, MAP did not increase, and GFR, ERPF, and UNaV fell much less than with L-NAME+IND alone (P < 0.02 to 0.001) with no differences versus PL or LOS alone. Angiotensin II blockade does not affect renal changes caused by L-NAME but prevents their potentiation by prostaglandin inhibition. Thus, endogenous prostaglandins counteract renal actions of endogenous angiotensin II in Na-repleted humans even when nitric oxide synthesis is inhibited.
Diversidad y estructura de franjas de árboles en una matriz agrícola (tolchés) en relación con la intensidad de uso forestal en Yaxcabá, Yucatán
Authors: Cortés-Sosa Diana M. , Levy-Tacher Samuel I. , Ramírez Marcial Neptalí , Navarrete Gutiérrez Darío A. , Rodríguez-Sánchez Perla Victoria 
Published: April 2021

Antecedentes: Los tolchés son franjas de árboles dentro del paisaje agrícola yucateco; proporcionan varios servicios ecosistémicos, incluyendo el suministro de material leñoso.
Preguntas e hipótesis: ¿Existen diferencias en la estructura de la vegetación leñosa de los tolchés a lo largo de la distancia al pueblo y la intensidad de uso? La hipótesis es que los atributos estructurales de la vegetación de los tolchés son afectados negativamente por la intensidad del uso del bosque, que disminuye de los márgenes del poblado, al área agrícola, hasta llegar a la vegetación madura.
Descripción de datos: Se evaluaron los atributos estructurales de tolchés y vegetación madura (VM), se realizó una rarefacción para estandarizar el esfuerzo de muestreo, un modelo lineal univariante seguido de pruebas a posteriori .
Sitio y años de estudio: El estudio se realizó en tolchés y VM de Yaxcabá, Yucatán, de febrero a julio de 2019.
Métodos: Se establecieron 44 unidades de muestreo (UM): 36 UM se ubicaron en tolchés, de 3 grupos diferenciados por su distancia al poblado y 8 UM se ubicaron en VM. Se registraron los individuos leñosos > 2.5 cm de DAP y se evaluaron las diferencias de los valores estructurales de ambas condiciones.
Resultados: La diversidad de los tolchés fueron significativamente superiores a la alcanzada por la VM, relacionada a la distancia al poblado.
Conclusiones: Se determinó la influencia de un patrón de aprovechamiento basado en la distancia al poblado. Niveles intermedios de aprovechamiento favorecen la riqueza y diversidad de los tolchés.
Connected Royal Oficios (offices): Furriera, Upholstery and Guardajoyas
Authors: Mateos Martín Mario , Benito García Pilar 
Published: April 2021

As a general rule, during 18th century the production and treatment of furniture within the Spanish court was determined by the characteristics of the raw materials used for its construction, mainly the wood and the textile.
The pre-eminance of one of these materials explained the management of furniture by different Oficios (offices) of the Royal Household. If wood was considered the key element, the furniture would be managed by the Furriera. When a textile was the most outstanding element, the Tapicería was the Oficio in charge of it. The presence of rich elements such as gold, silver or precious stones meant that the Guardajoyas was also involved. Therefore, it can be established that there was a close collaboration between the different workers of the Royal household.
However, materials were not always the reason why a furniture was going to be managed by one Oficio or another so, occasionally, function and/or type of furniture were also the key elements that determined its management.