bluuebunny/crossref_metadata_2025_split huggingface dataset:
Chemical quality parameters (water, protein, fat, NaCl ash and nitrites) in fermented sausage with the addition of Yersinia enterocolitica
Authors: Mitrovic R R , Jankovic V V , Ciric J S , Djordjevic V Z , Lj Juric Z , Mitrovic-Stanivuk M R , Baltic B M 
Published: November 2021

Abstract
The connection between diet and health was known to the oldest civilizations. Meat processing has influenced the increase of meat consumption, protein utilization and its energy value. Fermented sausages which are prepared in a safe way are rich in animal proteins and are an important part of the diet. Meat and adipose tissue are the basic ingredients in the production of fermented sausages, along with spices and salts, and other additives depending on the specifics of the product. Ripening is a process that separates raw sausages from all other types of sausages, and the quality of raw materials and their treatment directly affects the quality of the finished product, because the process of fermented sausage production is dominated by biological and biochemical processes. Different types of foods can cause food-borne illnesses, one of which is zoonotic yersiniosis caused by Yersinia enterocolitica. Due to the importance of Yersinia in meat, the European Food Safety Authority (EFSA) recommended that in addition to controlling the presence of Salmonella, it is mandatory to examine pork carcasses for the presence of Y. enterocolitica.
Biosensors for animal health and meat safety monitoring: farm-to-slaughterhouse continuum
Authors: Nastasijevic Ivan , Mitrovic Radmila , Jankovic Sasa 
Published: November 2021

Abstract
The meat supply chain needs to be managed for sufficient levels of consumer protection. Healthy animals are an essential precondition for a safe food supply, since zoonotic diseases, including meatborne pathogens, are a major threat to public health. Information about the livestock’s general health, animal welfare and prevalence of major meatborne hazards such as Salmonella, Campylobacter, STEC and Listeria monocytogenes is of utmost importance for effective biosecurity control on farm. Early detection of these hazards in faecal samples, monitoring blood levels of metabolites relevant for animal welfare (hormones) and animal health (acute phase proteins) can provide high-level control in the animal farming industry. Multiplex biosensors for pathogens and metabolites in the farm-to-slaughterhouse continuum constitute a practical and cost-efficient tool for early detection of signs related to meat safety. Point-of-care multiplex biosensors are an advantage versus commonly used methods ELISA and RT-PCR, since they provide possibilities for early detection and do not require expensive equipment, trained personnel or significant time for sample transfer and analyses. Biosensors can improve meat inspection and meat safety controls, and can serve as a primary tool for monitoring food safety parameters and contribute to the modernization of veterinary inspection and risk-based meat safety assurance system.
Categorization of animal feed according to microbiological quality - preferable improvement in the food chain
Authors: Nesic Ksenija , Mitrovic Radmila , Markovic Radmila 
Published: November 2021

Abstract
Given the fact that the law on animal feed in Serbia has long been expected and that the current Regulation on the quality of animal feed, which includes microbiological criteria, requires improvement over the years, it is time to choose the best new solutions. The recommendable change that would bring the categorization of animal feed according to more objective and comprehensive criteria is based on the use of the VDLUFA (Verbands Deutscher Landwirdschaftlicher Untersuchungs und Forschungsanstalten) method. In several European countries, it has become routine, due to the great interest of feed producers and animal breeders in good knowledge of microbiological quality of feed as a guarantee of a wholesome final product. It involves determination of the contents of moulds, yeasts and bacteria while taking into account their potential pathogenicity. Based on the number of microorganisms, divided into seven groups, feed and feedingstuffs are classified into four categories. Classes I to III can be placed on the market, while class IV is not suitable for animal nutrition. More precise, regular determination of microorganisms would also provide a better insight into other common feed-born problems, such as, for instance, the possibility of mycotoxin occurrence.
Health status and microbial quality of common carp reared in a pond fed with treated wastewater from a slaughterhouse
Authors: Pelic Milos , Novakov Nikolina , Djordjevic Vesna , Ljubojevic Pelic Dragana 
Published: November 2021

Abstract
Wastewater from slaughterhouses in many countries is still discharged into rivers, without having been adequately treated. Such wastewater contains plenty of organic matter which is an ideal source of nutrients for fish, but also for the development of microorganisms. Thus, usage of wastewater in aquaculture could become a health risk for humans, fish due to the introduction of microorganisms into the aquatic environment. In the available literature, there is insufficient data on health and meat safety regarding common carp reared in purified wastewater. The aim of this study was to assess the health and meat safety of common carp cultivated in a fishpond supplemented with slaughterhouse wastewater that was subjected to tertiary treatment. The number of parasites was not significant and not a single parasitic disease was found in this study, but the number of parasite species detected was as expected and typical for carp production. No spring viraemia of carp or koi herpesvirus disease was found. The carp cultivated were in good health and completely safe for human consumption in terms of the presence of microbial contaminants. The safe use of wastewater for fish rearing should be encouraged, but proper treatment of wastewater must be applied before its use.
Trichinellosis among the human population in Vojvodina
Authors: Petrovic J M , Prodanov-Radulovic J Z , Medic S N 
Published: November 2021

Abstract
The origin of the parasites of Trichinella species goes back to very ancient times–as far as the Paleozoic era, and the organism has been present in the territory of today’s Europe for millions of years. The first epidemic in the region of Serbia was reported in Zemun in 1923. Trichinellosis is the most important parasitic food borne disease in our country. In Vojvodina alone, 58 epidemic outbreaks were reported in the period 2015-2019 with 853 affected patients. The average incidence was 3.5 diseased individuals per 100 000 population. High morbidity and hospitalization rates and the occurrence of lethal outcomes qualifies trichinellosis as belonging to the category of relatively severe human diseases in Serbia. However, there are promising data indicating that implementation of relevant control measures and improvement of the awareness of the population have led to a statistically significant decrease in the number of diseased persons with trichinellosis in both Vojvodina and the entire country.
Green Economy and Meat Processing–Future Prospects
Authors: Petrovic Z , Milicevic D , Vranic D , Rajic S , Simunovic S 
Published: November 2021

Abstract
This paper provides a brief overview of the possible strategies for reducing hydrocarbon emissions from the meat industry according to the Green Deal program of the EU in the next decades. An overview of emerging technologies (high-pressure processing (HPP), shock wave technology (SW), ohmic heating (OH) and pulsed electric field (PEF), cultured meat) that should reduce gas emissions is given, as well as methodologies that can be applied (labelling, sustainable cooking, product lifecycle management (PLM) and product data management (PDM) applications). Noticeably, most novel strategies draw the conclusion that we should go for lower consumption of meat, especially beef, and change habits to eat and prepare foods in energy and environmentally friendly ways, as well as apply the so-called “green” food declaration in the future. Transforming into a climate-friendly economy, protecting biodiversity, and reorienting the agri-food industry growth can contribute to creating greater resilience of society.
Determination of patulin in apple juice by liquid chromatography-electrospray tandem mass spectrometry
Authors: Radicevic T , Jankovic S , Simunovic S , Stefanovic S , Petrovic Z , Jelusic V 
Published: November 2021

Abstract
Patulin is a mycotoxin produced by several fungi, (Penicillium, Aspergillus, Byssochlamys). The main sources of patulin intake in human diet are apples, apple juice and apple nectar, and for this reason, apple based foods are monitored for the presence of this mycotoxin. Commission Regulation EC No 1881/2006 lays down maximum residue limits (MRLs) of 50 µg/kg in apple juice and cider, 25 µg/kg in solid apple products, and 10 µg/kg in products for infants and young children. In Serbia, maximum permitted amounts of patulin in fruit juices, reconstituted concentrated fruit juices and fruit nectars, as well as in solid apple products, including apple compote and apple puree, intended for direct human consumption are prescribed in the Regulation on maximum concentrations of certain contaminants in foodstuffs. This paper presents the LC-MS/MS method for quantitative determination of patulin in apple juice. Criteria for method validation were taken from Commission Decision 2002/657/EC. Linearity was confirmed in the concentration ranges of 0-100 µg/kg, with the limit of detection (LoD) of 9.85 µg/kg. The performance of the method was successfully verified by participating in a proficiency study.
Intensive genetic selection and meat quality concerns in the modern broiler industry
Authors: Rajcic A , Baltic M Z , Brankovic Lazic I , Starcevic M , Baltic B M , Vucicevic I , Nesic S 
Published: November 2021

Abstract
The genetic selection toward bigger broilers provoked the development of muscular myopathies and abnormalities. Since the affected meat is downgraded and often inadequate for further processing, economic losses to the broiler industry are inevitable. In addition, not only the nutritional value of the meat has been decreased, but also sensory properties and technological traits seem to concern consumers more. This can have a very negative attitude toward poultry meat as consumers connect these traits with poor animal welfare. To avoid these problems in the future, new studies must be focused on identifying markers in live birds for newly developed myopathies. Also, better strategies for determining genetic factors, nutritional and slaughter conditions, and hence meat quality, are a complex concept, and all factors together have an impact on parameters of meat quality.
Optimization of pasteurization of meat products using pasteurization values (p-values)
Authors: Raseta M , Jovanovic J , Becskei Z , Brankovic Lazic I , Mrdovic B , Djordjevic V 
Published: November 2021

Abstract
This study aimed to examine the effect of shortening the effective heat treatment time by 15 minutes for finely and coarsely chopped sausages and canned meat in pieces packed in polyamide casings with diameters of 75 and 90 mm. Product safety was ensured in accordance with legislation and with the producer food safety system. Optimization led to a decrease in p-values for finely chopped sausages (diameter 90: 148.8 → 97.64 minutes; diameter 75: 111.2 → 58.4 min), coarsely chopped cooked sausages (diameter 90: 115.5 → 79.1 min; diameter 75: 245.3 → 106.6 min) and for canned meat in pieces (diameter 90: 249.7 → 95.91; diameter 75: 213.9 → 48.42 min). The use of p-value in optimization confirms the pasteurization level and ensures the safety of the product in the defined storage conditions. It is also possible to compare the levels of heat treatment for different products that are differently packaged.
Amino acids in animal feed: significance and determination techniques
Authors: Sefer M , Petronijevic R B , Trbovic D , Ciric J , Baltic T , Parunovic N , Djordjevic V 
Published: November 2021

Abstract
Amino acids are fundamental for animal nutrition. Their presence is necessary to maintain the normal structure and function of the intestine, and they are key in regulating metabolic pathways for improving health, survival, growth, development, lactation, and reproduction. The animal feed industry invests great resources and efforts to obtain optimal formulations in which the composition of amino acids plays a key role. In support of these aspirations in recent decades, much attention has been paid to the development and improvement of analytical techniques for the reliable, rapid and accurate determination of amino acid content in animal feed. This paper outlines different methodologies for the analysis of amino acid content in animal feed. Various methods, based on different analytical techniques, are presented for determination of amino acids in feed for nutritional and regulatory purposes.
Potential utilization of emulsion gels and multiple emulsions as delivery systems to produce healthier meat products
Authors: Serdaroğlu M 
Published: November 2021

Abstract
In recent years the increase in consumers’ demands for healthy food have accelerated the studies searching for innovative approaches in meat product formulations. Developing a healthier lipid profile and reducing fat are the most important goals in the meat industry. One of the main problems of animal fat replacement with plant oils is maintaining the technological and sensory properties of the products. Pre-emulsions provide a great opportunity to carry the healthier plant oils to meat systems for increasing mono and polyunsaturated fatty acid content, since adding liquid plant oils directly to product formulation can have technological and sensory problems. Using emulsion gels and multiple emulsions prepared with polyunsaturated oils could be a good option to achieve healthier meat products. This review addresses the emulsion gel and multiple emulsion properties and their use in meat products as fat replacers.
Uncertainty of measurement and conformity assessment
Authors: Stajkovic Silvana , Vasilev Dragan , Dimitrijevic Mirjana , Karabasil Nedjeljko 
Published: November 2021

Abstract
Knowledge of the measurement uncertainty of test results is fundamentally important for laboratories, their customers and all parties using and interpreting these results. In conformity assessment, a measurement result is used to decide if an item of interest conforms to a specified requirement. Because of measurement uncertainty, there is always the risk of incorrectly deciding whether or not an item conforms to a specified requirement based on the measured value of a property of the item. Conformity assessment can be quite challenging when the entity measured is so close to the tolerance limits of the specification that its uncertainty, however estimated, critically affects decision-making. In such cases, different decision rules can be used to make statements of conformity. The aim of this paper is to provide a survey of methods for the evaluation of measurement uncertainty in testing, as well as to stress the need for appropriate estimation of measurement uncertainty. This paper also aims to assist testing laboratories in understanding the different decision rules used in conformity assessment and level of risk (such as false accept and false reject) associated with the decision rule employed.
Extraction procedure optimization of the method for detecting ethylene oxide and 2-chloroethanol in sesame seed
Authors: Stefanovic S , Katanic N , Jankovic S 
Published: November 2021

Abstract
This study describes modifications of the extraction procedure within the European Union Reference method for determination of ethylene oxide and 2-chloroethanol in sesame seed. The method suggests utilisation of uniform stainless steel balls in order to facilitate extraction in small seed samples. Experiment was conducted with combination of balls of different sizes and the extraction efficacy was assessed by measurement of 2-chloroethanol in sesame seed samples from the local market. Increased efficacy of 18.3% for ethylene oxide and 16.2% for 2-chloroethanolwas observed when the combination of two diameters of balls was used compared to samples extracted by the guidelines in original method and alternative approach with uniform balls of the lower diameter..
Strategy for the study of the proteome in animal muscle tissue
Authors: Vostrikova N L , Chernukha I M , Khvostov D V , Zherdev A V 
Published: November 2021

Abstract
The existing approaches to the methodology of using proteomic and mass-selective methods for the analysis of the component composition of meat product proteins are systematized, formalized and modified. The scientific and practical foundations of a systemic proteomic strategy for identifying the protein composition of meat raw materials and the authenticity of meat products have been developed. A scheme for constructing proteomic maps and spectra of identified proteins and peptides of meat and meat products using bioinformatic data processing has been developed, and a scheme for choosing a research methodology as a tool for identifying and confirming the composition of meat products has been formed.
Prevalence, serovar, and antimicrobial resistance of Salmonella isolated from meat and minced meat used for production smoked sausage
Authors: Zaiko E V , Bataeva D S , Yushina Yu K , Grudistova M A , Velebit B 
Published: November 2021

Abstract
The objective of this study was to research the prevalence, serovars, and antimicrobial resistance profiles of Salmonella isolated from meat and minced meat used for the production of fermented sausage. A total of 116 samples were tested, and among them, 20 (17.2%) were positive. Salmonella was detected in 3 (10.3%) beef samples, 5 (19.2%) pork samples, and 6 (20.7%) poultry samples. In minced meat, the Salmonella prevalence was 18.8%. Salmonella enterica serovar Agama (5.2%) was the most commonly identified serovar, followed by S. Enteritidis (4.3%), S. Typhimurium (3.4%), S. Infantis (2.6%), and S. Lindenburg (1.7%). Most of the serovars identified in the present study are recognized as frequent causes of human salmonellosis. Thus, the presence of these serovars means foods with these meats are a likely source of human infections. We found the Salmonella isolates exhibited high rates of resistance to antimicrobials tetracycline, ampicillin, streptomycin, and ciprofloxacin. The highest level of resistance was to tetracycline (75%), followed by resistance to ampicillin (50%), streptomycin (30%), ciprofloxacin (20%), gentamicin (20%), and neomycin (10%). The high-level resistance observed for some of the serovars calls for concern. Salmonella with multidrug resistance in meat used to produce fermented sausages is considered a high additional risk for human health.
Five-Year Dynamic Prediction of Dementia Using Repeated Measures of Cognitive Tests and a Dependency Scale
Authors: Ben-Hassen Céline , Helmer Catherine , Berr Claudine , Jacqmin-Gadda Hélène 
Published: November 2021

Abstract
The progression of dementia prevalence over the years and the lack of efficient treatments to stop or reverse the cognitive decline make dementia a major public health challenge in the developed world. Identifying people at high risk of developing dementia could improve the treatment of these patients and help select the target population for preventive clinical trials. We used joint modeling to build a dynamic prediction tool of dementia based on the change over time of 2 neurocognitive tests (the Mini-Mental State Examination and the Isaacs Set Tests) as well as an autonomy scale (the Instrumental Activities of Daily Living). The model was estimated with data from the French cohort Personnes Agées QUID (1988–2015) and validated both by cross-validation and externally with data from the French Three City cohort (1999–2018). We evaluated its predictive abilities through area under the receiver operating characteristics curve and Brier score, accounting for right censoring and competing risk of death, and obtained an average area under the curve value of 0.95 for the risk of dementia in the next 5 or 10 years. This tool is able to discriminate a high-risk group of people from the rest of the population. This could be of help in clinical practice and research.
The Impact of Increasing the Minimum Legal Drinking Age from 18 to 20 Years in Lithuania on All-Cause Mortality in Young Adults—An Interrupted Time-Series Analysis
Authors: Tran Alexander , Jiang Huan , Lange Shannon , Livingston Michael , Manthey Jakob , Neufeld Maria , Room Robin , Štelemėkas Mindaugas , Telksnys Tadas , Petkevičienė Janina , Radišauskas Ričardas , Rehm Jürgen 
Published: November 2021

Abstract

Aims
To determine the effect of an alcohol policy change, which increased the minimum legal drinking age (MLDA) from 18 years of age to 20 years of age on all-cause mortality rates in young adults (18–19 years old) in Lithuania.

Methods
An interrupted time series analysis was conducted on a dataset from 2001 to 2019 (n = 228 months). The model tested the effects of the MLDA on all-cause mortality rates (deaths per 100,000 individuals) in three age categories (15–17 years old, 18–19 years old, 20–22 years old) in order to control for general mortality trends in young adults, and to isolate the effects of the MLDA from other alcohol control policies. Additional models that included GDP as a covariate and a taxation policy were tested as well.

Results
There was a significant effect of the MLDA on all-cause mortality rates in those 18–19 years old, when modelled alone. Additional analyses controlling for the mortality rate of other age groups showed similar findings. Inclusion of confounding factors (policies on alcohol taxation, GDP) eliminated the effects of MLDA.

Conclusions
Although there was a notable decline in all-cause mortality rates among young adults in Lithuania, a direct causal impact of MLDA on all-cause mortality rates in young adults was not definitively found.
iCRISEE: an integrative analysis of CRISPR screen by reducing false positive hits
Authors: Zhang Tengbo , Li Yaxu , Yang Yanrong , Weng Linjun , Wu Zhiqiang , Zhu Jiali , Qin Jieling , Liu Qi , Wang Ping 
Published: November 2021

Abstract
Clustered regularly interspaced short palindromic repeats associated protein 9 (CRISPR/Cas9) technology has become a popular tool for the study of genome function, and the use of this technology can achieve large-scale screening studies of specific phenotypes. Several analysis tools for CRISPR/Cas9 screening data have been developed, while high false positive rate remains a great challenge. To this end, we developed iCRISEE, an integrative analysis of CRISPR ScrEEn by reducing false positive hits. iCRISEE can dramatically reduce false positive hits and it is robust to different single guide RNA (sgRNA) library by introducing precise data filter and normalization, model selection and valid sgRNA number correction in data preprocessing, sgRNA ranking and gene ranking. Furthermore, a powerful web server has been presented to automatically complete the whole CRISPR/Cas9 screening analysis, where we integrated the main hypothesis in multiple algorithms as a full workflow, including quality control, sgRNA extracting, sgRNA alignment, sgRNA ranking, gene ranking and pathway enrichment. In addition, output of iCRISEE, including result mapping, sample clustering, sgRNA ranking and gene ranking, can be easily visualized and downloaded for publication. Taking together, iCRISEE presents to be the state-of-the-art and user-friendly tool for CRISPR screening data analysis. iCRISEE is available at https://www.icrisee.com.