bluuebunny/crossref_metadata_2025_split huggingface dataset:
Determination And Statistical Analysis of Atmospheric Deposition of Heavy Metals In Kosovo. A Moss Survey
Authors: Sopaj Flamur , Stafilov Trajče , Šajn Robert , Tašev Krste , Paçarizi Musaj 
Published: October 2021

Abstract
Atmospheric deposition of heavy metals on the territory of Kosovo was studied using the already widely used technique of mosses as biomonitors. This method is very convenient as it uses natural samples and thus avoids many difficulties associated with artificial samplers. Eight heavy metals (Al, Cd, Cr, Cu, Fe, Ni, Pb, and Zn) were determined in 45 moss samples. Statistical analysis was performed to better present and explain the data. High concentrations of Pb, Zn, Cd, and Ni were found near industrial sites and in more densely populated areas. Principal component analysis (PCA) identified more polluted sites such as Zveqan, Stanterg, Prapashticë, Siboc, and Lupç. It was also found that Pb, Zn, Cd, Cr, and Ni are the heavy metals that affect these polluted sites the most. High contents of Cd were found in Kaçanik and Paldemicë, Te Kalaja, Çikatovë, and Shalc, all sampling points found around industrial sights. The contamination factor (CF) and the polluted load index (PLI) were calculated. CF showed that only Cu, and Zn, had no or almost no contamination levels over the range of moss samples, while Cd and mainly Pb gave extremely high values for CF, indicating extreme contamination levels. The pollution load index also showed that only a few samples were slightly polluted, while most samples showed considerable and very high pollution levels.
Nonlocal Euler-Bernoulli Beam Theories With Material Nonlinearity and Their Application to Single-walled Carbon Nanotubes
Authors: Huang Kun , Qu Benning , Xu Wei , Yao Ji 
Published: October 2021

Abstract
The small-scale effect and the material nonlinearity significantly impact the mechanical properties of nanobeams. However, the combined effects of two factors have not attracted the attention of researchers. In the present paper, we proposed two new nonlocal theories to model mechanical properties of slender nanobeams for centroid locus stretching or inextensional effect respectively. Two new theories consider both the material nonlinearity and the small-scale effect induced by the nonlocal effect. The new models are used to analyze the static bending and the forced vibration for single-walled carbon nanotubes (SWCNTs). The results indicate that the stiffness softening effect induced by the material nonlinearity has more prominent impact than the nonlocal effect on SWCNT’s mechanical properties. Therefore, neglecting the material nonlinearity may cause qualitative mistakes.
Reactions of Some Varieties of Pearlmillet, Pennisetum glaucum (Linn.) R. Br. emend Stuntz. to Rice Moth, Corcyra cephalonica Staint.
Authors: Sharma Anita , Kumawat K C , Khinchi S K 
Published: October 2021

Abstract
Ten varieties of pearlmillet (RHB-173, RHB-177, MPMH-17, HHB-67, Pro Agro-9450, Pro Agro-9001, Pioneer 86 m 86, Pioneer 86 m 84, Pioneer 86 m 38 and Raj-171.) were evaluated for reaction to Corcyra cephalonica Staint. in the laboratory conditions. The adult emergence (F1), weight losses and susceptibility index in these varieties were ranged from 9.33-32.00, 10.44–31.69% and 2.77–6.82, respectively, the minimum in Pioneer 86 m 86 and maximum in Raj-171; other varieties were in the middle order. The seed damage recorded in different varieties ranged from 23.67–75.84%, the minimum in Pioneer 86 m 86 (23.67%), Pro Agro-9450 (25.55%) and Pro Agro-9001 (28.33%), these treatments were found at par each other. The Raj-171 was found to be badly infested (75.84% grain damage) which differed significantly over other varieties. The developmental period of the pest varied from 50.88–80.61 days in different varieties, minimum being in Raj-171 and maximum in Pioneer 86 m 86.
Bacteriophage EK99P-1 Alleviates Enterotoxigenic Escherichia Coli K99-Induced Barrier Dysfunction and Inflammation
Authors: Kim Narae , Gu Min jeong , Kye Yoon-Chul , Ju Young-Jun , Hong Rira , Ju Do Bin , Pyung Young Jin , Han Seung Hyun , Park Byung-Chul , Yun Cheol-Heui 
Published: October 2021

Abstract
Bacteriophages have long been used as a potential alternative to antibiotics for livestock due to their ability to specifically kill ETEC, which are a major cause of diarrhea in piglets. However, the control of ETEC infection by phages within intestinal epithelial cells, and their relationship with host immune responses, remain poorly understood. In this study, we evaluated the effect of phage EK99P-1 against ETEC K99-infected IPEC-J2. Phage EK99P-1 prevented ETEC K99-induced barrier disruption by attenuating the increased permeability mediated by the loss of tight junction proteins such as ZO-1, occludin, and claudin-3. ETEC K99-induced inflammatory responses, such as IL-8 secretion, were decreased by treatment with phage EK99P-1. We used a IPEC-J2/PBMC Transwell co-culture system to investigate whether the modulation of barrier disruption and chemokine secretion by phage EK99P-1 in ETEC K99-infected IPEC-J2 would influence basolateral immune cells. The results showed that phage EK99P-1 reduced the mRNA expression of ETEC K99-induced pro-inflammatory cytokines, IL-1β and IL-8, from PBMC collected on the basolateral side. Together, these results suggest that phage EK99P-1 prevented ETEC K99-induced barrier dysfunction in IPEC-J2 and alleviated inflammation caused by ETEC K99 infection. Reinforcement of the intestinal barrier by phage EK99P-1 also modulates the immune cell inflammatory response.
An Integrative Multi-omics Analysis based on Liquid-liquid Phase Separation Delineates Distinct Subtypes of Lower-grade Glioma and Identifies a Prognostic Signature
Authors: Zheng Jianglin , Wu Zhipeng , Qiu Yue , Wang Xuan , Jiang Xiaobing 
Published: October 2021

Abstract
Background: Emerging evidences have indicated that the aberrant liquid-liquid phase separation (LLPS) leads to the dysfunction of biomolecular condensates, thereby contributing to the tumorigenesis and progression. Nevertheless, it remains unclear whether or how the LLPS of specific molecules affects the prognosis and tumor immune microenvironment (TIME) of patients with lower-grade glioma (LGG).Methods: We integrated the transcriptome information of 3585 LLPS-related genes to comprehensively evaluate the LLPS patterns of 423 patients with LGG in The Cancer Genome Atlas (TCGA) cohort. Then, we systematically demonstrated the differences among four LLPS subtypes based on multi-omics analyses. In addition, we constructed the LLPS-related prognostic risk score (LPRS) for individualized integrative assessment. Results: Based on the expression profiles of 85 scaffolds, 355 regulators, and 3145 clients in LGG, we identified four LLPS subtypes, namely LS1, LS2, LS3 and LS4. We confirmed that there were significant differences in prognosis, clinicopathological features, cancer hallmarks, genomic alterations, TIME patterns and immunotherapeutic responses among four LLPS subtypes. In addition, a prognostic signature called LPRS was constructed for individualized integrative assessment. LPRS exhibited a robust predictive capacity for prognosis of LGG patients in multiple cohorts. Moreover, LPRS was found to be correlated with clinicopathological features, cancer hallmarks, genomic alterations and TIME patterns of LGG patients. The predictive power of LPRS in response to immune checkpoint inhibitor (ICI) therapy was also prominent.Conclusions: This study provided a novel classification of LGG patients based on LLPS. The constructed LPRS might facilitate individualized prognosis prediction and better immunotherapy options for LGG patients.
Is Prophylactic Hyperthermic Intraperitoneal Chemotherapy Beneficial to The Long-Term Survival of Patients After Radical Gastric Cancer Surgery: A Systematic Review and Meta-analysis
Authors: Zhuang Xuhui , He Yuewen , Ma Wuhua 
Published: October 2021

Abstract
Background: Hyperthermic intraperitoneal chemotherapy (HIPEC) has been proven to improve the survival rate of gastric cancer and reduce peritoneal recurrence. We aimed to evaluate the effectiveness and safety of prophylactic HIPEC after radical gastric cancer surgery in this study.Methods: Researchers searched for studies published in PubMed, Embase, Web of science, Scopus, Cochrane, Clinicalkey databases and Microsoft Academic databases to identify studies that examine the impact of prophylactic HIPEC on the survival, recurrence and adverse events of patients undergoing radical gastric cancer surgery. RevMan 5.3 was used to analyze the results and risk of bias. The PROSERO registration number is CRD42021262016.Results: This meta-analysis included 19 studies with a total of 2097 patients, 12 of which are RCTs. The results showed that the 1-,3-and 5-year overall survival rate was significantly favorable to HIPEC (OR=5.10,2.47,1.96 respectively). Compared with the control group, the overall recurrence rate and peritoneal recurrence rate of the HIPEC group were significantly lower (OR=0.43,0.26 respectively). Significantly favorable to the control group in terms of renal dysfunction and pulmonary dysfunction complications(OR=2.44,6.03 respectively). Regarding the causes of death due to postoperative recurrence: liver recurrence, lymph node and local recurrence and peritoneal recurrence, the overall effect is not significantly different (OR=0.81,1.19,0.37 respectively).Conclusions: 1-,3-and 5-year overall survival follow-up can be incremented by the prophylactic HIPEC, and which can significantly reduce the overall recurrence rate and peritoneal recurrence rate. HIPEC can cause significant pulmonary dysfunction and renal dysfunction complications. No difference has been found in the deaths due to recurrence after surgery.
Susceptibility of different life stages of Callosobruchus maculatus and Callosobruchus chinensis to ECO2FUME gas and its impact on cowpea seeds quality
Authors: Amin Manar Y. , Abotaleb Abeer Omar , Mohamed Refaat A. 
Published: October 2021

Abstract
ECO2FUME® phosphine fumigant was used to fumigate stored cowpea piles under gas-proof sheets to assess its performance against different developmental stages of Callosobruchus maculatus and Callosobruchus chinensis. The mortality was determined on four developmental stages of C. maculatus and C. chinensis, employing ECO2FUME at different concentrations 25, 30, 40, and 50 g/m3 (2% PH3 and 98% CO2 by weight) for 3-days. All stages of both insect species in packed cowpea stacks were completely controlled at 3-days when applied with an ECO2FUME application rate of 50 g/m3. Cases of pupae of C. maculatus and C. chinensis exhibit the highest resistance than other stages, with 78.2 and 73.93% mortality respectively, at 40 g/m3 after 3-days post-exposure to ECO2FUME. Suppression of F1 generation was obtained after fumigation with the same concentration (50 g/m3). Quality (in terms of cowpea germination) and all chemical constituents of cowpea seeds were non significantly (P ≤ 0.05) affected by the fumigation concentration of 50 g/m3.
Molecular Epidemiological Study of Porcine Epidemic Diarrhea from Winter 2020 to Spring 2021 in Jiangsu Province
Authors: Zhu Tingfan , Qian Jinhan , Shen Zijun , Shao Hongxia , qian Kun , Jin Wenjie , Qin Aijian 
Published: October 2021

Abstract
Background: Porcine epidemic diarrhea (PED) is an acute and highly contagious infectious disease caused by the porcine epidemic diarrhea virus (PEDV) that occurs most frequently from winter to spring. It is associated with high morbidity and mortality rates, especially among piglets, and causes huge losses in the pig industry. The aim of this molecular epidemiological study was to identify the current strains of PEDV that are prevalent in Jiangsu Province, China.Methods: From winter 2020 to spring 2021, 793 small intestine tissue, fecal, and anal swab samples were collected from 72 pig farms in 11 counties in the jurisdiction of 5 regions of Jiangsu Province (Yancheng, Suqian, Changzhou, Xuzhou, and Yangzhou). A highly variable region of the S gene was amplified and sequenced, and phylogenetic analysis was conducted to compare this sequence with corresponding sequences from reference strains deposited in GenBank. Results: A total of 457 samples from 57 pig farms were positive for PEDV: this implies a positivity rate of 79% (57/72) for pig farms and a sample positivity rate of 57.6% (457/793). The positivity rates were 78% (107/137) in Yancheng, 53% (218/409) in Suqian, 48% (94/195) in Changzhou, 80% (16/20) in Xuzhou, and 88% (14/16) in Yangzhou. Seven representative samples were selected for sequencing, and phylogenetic analysis showed that the seven isolated strains exhibited 88.0%–100% nucleotide identity and 87.3%–99% amino acid identity. Additionally, our isolates exhibited 88.3%–99.7% nucleotide identity and 88%–98.5% amino acid identity with the reference PEDV strains. Phylogenetic tree analysis indicated that there were considerable difference in the sources of the variants.Conclusions: PEDV had a high infection rate among pigs and is possibly the main pathogenic agent of pig diarrhea in Jiangsu province. Importantly, vaccines must be screened for their efficacy against the newly identified variants.
Obstetric and Perinatal Outcomes in Parturients with Active SARS-CoV-2 Infection During Labor and Delivery: a Retrospective Cohort Study
Authors: Zlatkin Rita , Dollinger Sarah , Jacoby Chen , Shmueli Anat , Barbash-Hazan Shiri , Chen Rony , Wiznitzer Arnon , Hadar Eran 
Published: October 2021

Abstract
Background: The COVID-19 pandemic is an ongoing global healthcare crisis that negatively affects pregnant women. Although patients with an acute infection during pregnancy have been widely studied, information regarding labor and delivery while infected is sparse. The aim of the study was to ascertain maternal, obstetrical, and perinatal outcomes of women who gave birth while infected with SARS-CoV-2. Methods: Patients diagnosed with COVID-19 during pregnancy at a tertiary medical center in 4/20-2/21 were identified by a retrospective database search. Those with an active intrapartum SARS-CoV-2 infection were compared with those who recovered at least 10 days before labor and delivery. Results: Of the 176 women included in the study, 84 had a SARS-CoV-2 infection at the time of delivery and 92 had recovered from the infection. There was no statistically significant between-group difference in mean gestational age at delivery (39 weeks for both, p=0.71) and overall rate of cesarean delivery (26.2% vs 17.4%, respectively, p=0.35) or non-elective cesarean delivery (10.71% vs 4.34%, respectively, p=0.48). In the active-infection group, the rate of severe disease was 2.4%, and of critical disease (with intensive care unit admission, mechanical ventilation, and ECMO), 3.6%, compared to zero for both in the recovered group. No differences were found between the groups in adverse perinatal outcomes. Conclusion: Delivery is safe and feasible in women with active SARS-CoV-2 infection. Nevertheless, we found a non-significant trend for more severe disease and for cesarean delivery and urgent cesarean delivery (for COVID-19-related indications) in women with an intrapartum SARS-CoV-2 infection.
Lack of miRNA-17 family mediates high glucose-induced PAR-1 up-regulation in glomerular mesangial cells
Authors: Tang Zhuang-Zhuang , Gu Pan-Pan , An Xiao-Fei , Gou Ling-Shan , Liu Yao-Wu 
Published: October 2021

Abstract
Up-regulation of thrombin receptor protease-activated receptor 1 (PAR-1) is verified to contribute to chronic kidney diseases, including diabetic nephropathy, however, the mechanisms are still unclear. In this study, we investigated the effect of PAR-1 on high glucose-induced proliferation of human glomerular mesangial cells (HMCs), and explored the mechanism of PAR-1 up-regulation from alteration of microRNAs. We found that high glucose stimulated proliferation of the mesangial cells whereas PAR-1 inhibition with vorapaxar attenuated the cell proliferation. Moreover, high glucose up-regulated PAR-1 in mRNA level and protein expression while did not affect the enzymatic activity of thrombin in HMCs after 48 h culture. Then high glucose induced PAR-1 elevation was likely due to the alteration of the transcription or post-transcriptional processing. It was found that miR-17 family members including miR-17-5p, -20a-5p, and − 93-5p were markedly decreased among the eight detected microRNAs only in high glucose-cultured HMCs, but miR-129-5p, miR-181a-5p, and miR-181b-5p were markedly decreased in both high glucose-cultured HMCs and osmotic press control compared with normal glucose culture. So miR-20a was selected to confirm the role of miR-17 family on PAR-1 up-regulation, finding that miR-20a-5p overexpression reversed the up-regulation of PAR-1 in mRNA and protein levels induced by high glucose in HMCs. In summary, our finding indicated that PAR-1 up-regulation mediated proliferation of glomerular mesangial cells induced by high glucose, and deficiency of miR-17 family resulted in PAR-1 up-regulation.
Peer Support for People with Chronic Conditions: A Systematic Review of Reviews
Authors: Thompson Dean M. , Booth Lesley , Mathers Jonathan 
Published: October 2021

Abstract
Background: People with chronic conditions experience functional impairment, lower quality of life, and greater economic hardship and poverty. Social isolation and loneliness are common for people with chronic conditions, with multiple co-occurring chronic conditions predicting an increased risk of loneliness. Peer support is a socially driven intervention involving people with lived experience of a condition helping others to manage the same condition, potentially offering a sense of connectedness and purpose, and experiential knowledge to manage disease. However, it is unclear what outcomes are important to patients across the spectrum of chronic conditions, what works and for whom. The aims of this review were to (1) collate peer support intervention components, (2) collate the outcome domains used to evaluate peer support, (3) synthesise evidence of effectiveness, and (4) identify the mechanisms of effect, for people with chronic conditions.Methods: A systematic review of reviews was conducted. Reviews were included if they reported on formal peer support between adults or children with one or more chronic condition. Data were analysed using narrative synthesis.Results: The search identified 6222 unique publications. Thirty-one publications were eligible for inclusion. Components of peer support were organised into nine categories: social support, psychological support, practical support, empowerment, condition monitoring and treatment adherence, informational support, behavioural change, encouragement and motivation, and physical training. Fifty-five outcome domains were identified. Quality of life, and self-efficacy were the most measured outcome domains identified. Most reviews reported positive but non-significant effects.Conclusions: The effectiveness of peer support is unclear and there are inconsistencies in how peers are defined, a lack of clarity in research design and intervention reporting, and widely variable outcome measurement. This review presents a range of components of peer support interventions that may be of interest to clinicians developing new support programmes. However, it is unclear precisely what components to use and with whom. Therefore, implementation of support in different clinical settings may benefit from participatory action research so that services may reflect local need.
The Expression of LIMK1 is Related to the Poor Prognosis and Immune Function of Hepatocellular Carcinoma
Authors: Peng Yisheng , Fan Jun , Zhu Gang , Tan Shunde , Chen Jianfei , Wang Xuewen , Gou Haoxian , Su Song , Yang Xiaoli , Li Bo 
Published: October 2021

Abstract
Background: According to reports, LIMK1 may have the effect of promoting tumor progression. However, the effect of the expression of LIMK1 on the healing of patients with hepatocellular carcinoma and its effect on the immune function are still not clear. Therefore, we analyzed the effect of LIMK1 on the healing of patients with hepatocellular carcinoma and its correlation with immunity through bioinformatics analysis.Methods: Download the transcriptional expression profile of LIMK1 in hepatocellular carcinoma tissues and normal tissues in TCGA, and study its expression in hepatocellular carcinoma. Study the expression of LIMK1 in hepatocellular carcinoma through CPTAC and HPA database. The Kaplan-Meier method was used to evaluate the effect of LIMK1 expression on the survival of patients with hepatocellular carcinoma. Use the STRING database to construct a protein-protein interaction (PPI) network. Use the "ClusterProfiler" package for feature-rich analysis. Use TISIDB database and Xiantao platform to study the relationship between LIMK1 mRNA expression and immune infiltration.Results: The expression of LIMK1 in hepatocellular carcinoma tissues was significantly up-regulated. Increased expression of LIMK1 mRNA is related to high TNM staging. In the ROC curve, when the cut-off level is 1.813, the sensitivity and specificity of LIMK1 to distinguish hepatocellular carcinoma from adjacent controls are 80.7% and 86%, respectively.The Kaplan-Meier curve shows that the higher the expression of LIMK1, the worse the survival of patients with hepatocellular carcinoma (42.2 months vs. 70 months, P = 0.001). Correlation analysis studies have shown that the expression of LIMK1 mRNA in hepatocellular carcinoma is related to immune cell infiltration.Conclusion: Up-regulation of LIMK1 may affect the survival rate and immune invasion of hepatocellular carcinoma. Studies have shown that LIMK1 may be related to the poor prognosis of hepatocellular carcinoma, and has a certain relationship with the immune infiltration of hepatocellular carcinoma.