bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cardiovascular RNA markers and artificial intelligence may improve COVID-19 outcome: a position paper from the EU-CardioRNA COST Action CA17129
Authors: Badimon Lina , Robinson Emma L , Jusic Amela , Carpusca Irina , deWindt Leon J , Emanueli Costanza , Ferdinandy Péter , Gu Wei , Gyöngyösi Mariann , Hackl Matthias , Karaduzovic-Hadziabdic Kanita , Lustrek Mitja , Martelli Fabio , Nham Eric , Potočnjak Ines , Satagopam Venkata , Schneider Reinhard , Thum Thomas , Devaux Yvan 
Published: April 2021

AbstractThe coronavirus disease 2019 (COVID-19) pandemic has been as unprecedented as unexpected, affecting more than 105 million people worldwide as of 8 February 2020 and causing more than 2.3 million deaths according to the World Health Organization (WHO). Not only affecting the lungs but also provoking acute respiratory distress, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is able to infect multiple cell types including cardiac and vascular cells. Hence a significant proportion of infected patients develop cardiac events, such as arrhythmias and heart failure. Patients with cardiovascular comorbidities are at highest risk of cardiac death. To face the pandemic and limit its burden, health authorities have launched several fast-track calls for research projects aiming to develop rapid strategies to combat the disease, as well as longer-term projects to prepare for the future. Biomarkers have the possibility to aid in clinical decision-making and tailoring healthcare in order to improve patient quality of life. The biomarker potential of circulating RNAs has been recognized in several disease conditions, including cardiovascular disease. RNA biomarkers may be useful in the current COVID-19 situation. The discovery, validation, and marketing of novel biomarkers, including RNA biomarkers, require multi-centre studies by large and interdisciplinary collaborative networks, involving both the academia and the industry. Here, members of the EU-CardioRNA COST Action CA17129 summarize the current knowledge about the strain that COVID-19 places on the cardiovascular system and discuss how RNA biomarkers can aid to limit this burden. They present the benefits and challenges of the discovery of novel RNA biomarkers, the need for networking efforts, and the added value of artificial intelligence to achieve reliable advances.
Colonic Strictures in Inflammatory Bowel Disease: Epidemiology, Complications, and Management
Authors: Fumery Mathurin , Yzet Clara , Chatelain Denis , Yzet Thierry , Brazier Franck , LeMouel Jean-Philippe , Laharie David , Sabbagh Charles 
Published: April 2021

Abstract
The management of colorectal stricture complicating inflammatory bowel disease [IBD] remains a challenging condition. Stricture raises concern about neoplastic complications, which cannot be fully ruled out by negative endoscopic biopsies. Also, impassable strictures restrict the endoscopic monitoring of upstream disease activity and dysplasia. Surgery remains the ‘gold standard’ treatment for colonic strictures but is associated with high morbidity. Over the past few decades, our therapeutic arsenal for IBD has been reinforced by biologics and therapeutic endoscopy. Few studies have focused on colonic strictures, and so current therapeutic strategies are based on a low level of evidence and applied by analogy with the treatment of ileal strictures. With a view to facilitating the decision making process in clinical practice, we reviewed the literature on the epidemiology, natural history, and management of colonic strictures in IBD.
Efficiency and outcome quality of Herbst-Multibracket appliance therapy in Class II:2 patients
Authors: Bock Niko Christian , Killat Sophia , Ruf Sabine 
Published: April 2021

Summary

Objectives
The aim of this retrospective investigation was to generate representative data on the efficiency and outcome quality of Class II:2 Herbst-Multibracket appliance (Herbst-MBA) treatment.

Subjects and methods
All Class II:2 patients who had started Herbst-MBA treatment at the study centre since 1986 were included. Study casts from before treatment, after Herbst-MBA treatment, and (if available) after ≥24 months of follow-up were evaluated using standard occlusal variables, the Peer Assessment Rating (PAR)-Index, and the Ahlgren-Scale.

Results
During treatment, the pre-treatment PAR score was reduced from 23.6 ± 7.66 to 5.6 ± 3.85 (n = 192); during the follow-up period, a slight increase to 6.0 ± 3.60 occurred (n = 127). The percentage of patients who could be assigned to the PAR category ‘greatly improved’ was 30% after treatment and 33% after follow-up; only 1%, respectively, 2% had to be assigned to the category ‘worse/no different’. The outcome ratings according to the Ahlgren-Scale revealed 20% excellent, 32% good, 46% acceptable, and 2% unsuccessful results.

Limitations
Retrospective study design with follow-up data not available from all subjects and no data from untreated controls.

Conclusions
Herbst-MBA is an efficient treatment approach in orthodontic care of Class II:2 malocclusions. High-quality results (mean/median PAR score: 5.6/5.0) with very good short-term stability (2.2 years’ follow-up) were achieved. Unfortunately, no appropriate control group or groups, where other treatment approaches had been applied, exist.
Inhibitors of DNA topoisomerases I and II applied to Candida dubliniensis reduce growth, viability, the generation of petite mutants and toxicity, while acting synergistically with fluconazole
Authors: Tagle-Olmedo Tania , Andrade-Pavón Dulce , Martínez-Gamboa Areli , Gómez-García Omar , García-Sierra Francisco , Hernández-Rodríguez César , Villa-Tanaca Lourdes 
Published: April 2021

ABSTRACT
The increasing resistance of Candida species to azoles emphasizes the urgent need for new antifungal agents with novel mechanisms of action. The aim of this study was to examine the effect of three DNA topoisomerase inhibitors of plant origin (camptothecin, etoposide and curcumin) on the growth of Candida dubliniensis. The phylogenetic analysis showed a close relationship between the topoisomerase enzymes of C. dubliniensis and Candida albicans. The alignment of the amino acid sequences of topoisomerase I and II of yeasts and humans evidenced conserved domains. The docking study revealed affinity of the test compounds for the active site of topoisomerase I and II in C. dubliniensis. Curcumin and camptothecin demonstrated a stronger in vitro antifungal effect than the reference drugs (fluconazole and itraconazole). Significant synergistic activity between the topoisomerase inhibitors and fluconazole at the highest concentration (750 µM) was observed. Fluconazole induced the petite phenotype to a greater degree than the topoisomerase inhibitors, indicating a tendency to generate resistance. Lower toxicity was found for such inhibitors versus reference drugs on Galleria mellonella larva. The topoisomerase inhibitors exhibited promising antifungal activity, and the DNA topoisomerase enzymes of C. dubliniensis proved to be an excellent model for evaluating new antifungal compounds.
Population Genomics of the Maize PathogenUstilago maydis: Demographic History and Role of Virulence Clusters in Adaptation
Authors: Schweizer Gabriel , Haider Muhammad Bilal , Barroso Gustavo V , Rössel Nicole , Münch Karin , Kahmann Regine , Dutheil Julien Y 
Published: April 2021

AbstractThe tight interaction between pathogens and their hosts results in reciprocal selective forces that impact the genetic diversity of the interacting species. The footprints of this selection differ between pathosystems because of distinct life-history traits, demographic histories, or genome architectures. Here, we studied the genome-wide patterns of genetic diversity of 22 isolates of the causative agent of the corn smut disease, Ustilago maydis, originating from five locations in Mexico, the presumed center of origin of this species. In this species, many genes encoding secreted effector proteins reside in so-called virulence clusters in the genome, an arrangement that is so far not found in other filamentous plant pathogens. Using a combination of population genomic statistical analyses, we assessed the geographical, historical, and genome-wide variation of genetic diversity in this fungal pathogen.We report evidence of two partially admixed subpopulations that are only loosely associated with geographic origin. Using the multiple sequentially Markov coalescent model, we inferred the demographic history of the two pathogen subpopulations over the last 0.5 Myr. We show that both populations experienced a recent strong bottleneck starting around 10,000 years ago, coinciding with the assumed time of maize domestication. Although the genome average genetic diversity is low compared with other fungal pathogens, we estimated that the rate of nonsynonymous adaptive substitutions is three times higher in genes located within virulence clusters compared with nonclustered genes, including nonclustered effector genes. These results highlight the role that these singular genomic regions play in the evolution of this pathogen.
The rec8 gene of Schizosaccharomyces pombe is involved in linear element formation, chromosome pairing and sister-chromatid cohesion during meiosis.
Authors: Molnar M , Bähler J , Sipiczki M , Kohli J 
Published: April 2021

Abstract
The fission yeast Schizosaccharomyces pombe does not form tripartite synaptonemal complexes during meiotic prophase, but axial core-like structures (linear elements). To probe the relationship between meiotic recombination and the structure, pairing, and segregation of meiotic chromosomes, we genetically and cytologically characterized the rec8-110 mutant, which is partially deficient in meiotic recombination. The pattern of spore viability indicates that chromosome segregation is affected in the mutant. A detailed segregational analysis in the rec8-110 mutant revealed more spores disomic for chromosome III than in a wild-type strain. Aberrant segregations are caused by precocious segregation of sister chromatids at meiosis I, rather than by nondisjunction as a consequence of lack of crossovers. In situ hybridization further showed that the sister chromatids are separated prematurely during meiotic prophase. Moreover, the mutant forms aberrant linear elements and shows a shortened meiotic prophase. Meiotic chromosome pairing in interstitial and centromeric regions is strongly impaired in rec8-110, whereas the chromosome ends are less deficient in pairing. We propose that the rec8 gene encodes a protein required for linear element formation and that the different phenotypes of rec8-110 reflect direct and indirect consequences of the absence of regular linear elements.
A Microsatellite Linkage Map of Rainbow Trout (Oncorhynchus mykiss) Characterized by Large Sex-Specific Differences in Recombination Rates
Authors: Sakamoto Takashi , Danzmann Roy G , Gharbi Karim , Howard Pamela , Ozaki Akiyuki , Khoo Sok Kean , Woram Rachael A , Okamoto Nobuaki , Ferguson Moira M , Holm Lars-Erik , Guyomard René , Hoyheim Bjorn 
Published: April 2021

Abstract
We constructed a genetic linkage map for a tetraploid derivative species, the rainbow trout (Oncorhynchus mykiss), using 191 microsatellite, 3 RAPD, 7 ESMP, and 7 allozyme markers in three backcross families. The linkage map consists of 29 linkage groups with potential arm displacements in the female map due to male-specific pseudolinkage arrangements. Synteny of duplicated microsatellite markers was used to identify and confirm some previously reported pseudolinkage arrangements based upon allozyme markers. Fifteen centromeric regions (20 chromosome arms) were identified with a half-tetrad analysis using gynogenetic diploids. Female map length is ~10 M, but this is a large underestimate as many genotyped segments remain unassigned at a LOD threshold of 3.0. Extreme differences in female:male map distances were observed (ratio F:M, 3.25:1). Females had much lower recombination rates (0.14:1) in telomeric regions than males, while recombination rates were much higher in females within regions proximal to the centromere (F:M, 10:1). Quadrivalent formations that appear almost exclusively in males are postulated to account for the observed differences.
Perceiving Dynamic Emotions Expressed Simultaneously in the Face and Body Minimizes Perceptual Differences Between Young and Older Adults
Authors: Abo Foul Yasmin , Eitan Renana , Mortillaro Marcello , Aviezer Hillel 
Published: April 2021

Abstract

Objectives
It is commonly argued that older adults show difficulties in standardized tasks of emotional expression perception, yet most previous works relied on classic sets of static, decontextualized, and stereotypical facial expressions. In real life, facial expressions are dynamic and embedded in a rich context, 2 key factors that may aid emotion perception. Specifically, body language provides important affective cues that may disambiguate facial movements.

Method
We compared emotion perception of dynamic faces, bodies, and their combination in a sample of older (age 60–83, n = 126) and young (age 18–30, n = 124) adults. We used the Geneva Multimodal Emotion Portrayals set, which includes a full view of expressers’ faces and bodies, displaying a diverse range of positive and negative emotions, portrayed dynamically and holistically in a nonstereotypical, unconstrained manner. Critically, we digitally manipulated the dynamic cue such that perceivers viewed isolated faces (without bodies), isolated bodies (without faces), or faces with bodies.

Results
Older adults showed better perception of positive and negative dynamic facial expressions, while young adults showed better perception of positive isolated dynamic bodily expressions. Importantly, emotion perception of faces with bodies was comparable across ages.

Discussion
Dynamic emotion perception in young and older adults may be more similar than previously assumed, especially when the task is more realistic and ecological. Our results emphasize the importance of contextualized and ecological tasks in emotion perception across ages.
Ectopic expression of CGG-repeats alters ovarian response to gonadotropins and leads to infertility in a murine FMR1 premutation model
Authors: Shelly Katharine E , Candelaria Nicholes R , Li Ziyi , Allen Emily G , Jin Peng , Nelson David L 
Published: April 2021

Abstract
Women heterozygous for an expansion of CGG repeats in the 5’UTR of FMR1 risk developing fragile X-associated primary ovarian insufficiency (FXPOI) and/or tremor and ataxia syndrome (FXTAS). We show that expanded CGGs, independent of FMR1, are sufficient to drive ovarian insufficiency and that expression of CGG-containing mRNAs alone or in conjunction with a polyglycine-containing peptide translated from these RNAs contribute to dysfunction. Heterozygous females from two mouse lines expressing either CGG RNA-only (RNA-only) or CGG RNA and the polyglycine product FMRpolyG (FMRpolyG+RNA) were used to assess ovarian function in aging animals. The expression of FMRpolyG+RNA led to early cessation of breeding, ovulation and transcriptomic changes affecting cholesterol and steroid hormone biosynthesis. Females expressing CGG RNA-only did not exhibit decreased progeny during natural breeding, but their ovarian transcriptomes were enriched for alterations in cholesterol and lipid biosynthesis. The enrichment of CGG RNA-only ovaries for differentially expressed genes related to cholesterol processing provided a link to the ovarian cysts observed in both CGG-expressing lines. Early changes in transcriptome profiles led us to measure ovarian function in prepubertal females that revealed deficiencies in ovulatory responses to gonadotropins. These include impairments in cumulus expansion and resumption of oocyte meiosis, as well as reduced ovulated oocyte number. Cumulatively, we demonstrated the sufficiency of ectopically expressed CGG repeats to lead to ovarian insufficiency and that co-expression of CGG-RNA and FMRpolyG lead to premature cessation of breeding. However, the expression of CGG RNA-alone was sufficient to lead to ovarian dysfunction by impairing responses to hormonal stimulation.
Percutaneous major aortopulmonary collateral artery banding using a covered stent in an infant
Authors: Nagatomo Yusaku , Nagata Hazumu , Yamamura Kenichiro , Ohga Shouichi 
Published: April 2021

Abstract
We report a 6-month-old infant with pulmonary atresia with ventricular septal defect who was successfully treated with a novel technique of percutaneous major aortopulmonary collateral artery (MAPCA) banding with a covered stent. He suffered from heart failure due to a residual MAPCA of 4.5 mm in diameter. A covered stent tied with 2 5–0 size nylon threads was successfully deployed into the MAPCA in a dumbbell shape with a banding diameter of 2.8 mm. Banding covered stent implantation is a useful method to reduce the pulmonary blood flow in patients with MAPCA, which could be a less-invasive alternative to surgery.
Apramycin resistance in epidemic carbapenem-resistant Klebsiella pneumoniae ST258 strains
Authors: Hao Mingju , Schuyler Jessica , Zhang Haifang , Shashkina Elena , Du Hong , Fouts Derrick E , Satlin Michael , Kreiswirth Barry N , Chen Liang 
Published: April 2021

Abstract

Background
Recent studies indicated that the monosubstituted deoxystreptamine aminoglycoside apramycin is a potent antibiotic against a wide range of MDR Gram-negative pathogens.

Objectives
To evaluate the in vitro activity of apramycin against carbapenem-resistant Klebsiella pneumoniae (CRKp) isolates from New York and New Jersey, and to explore mechanisms of apramycin resistance.

Methods
Apramycin MICs were determined by broth microdilution for 155 CRKp bloodstream isolates collected from 2013 to 2018. MLST STs, wzi capsular types and apramycin resistance gene aac(3’)-IV were examined by PCR and Sanger sequencing. Selected isolates were further characterized by conjugation experiments and WGS.

Results
Apramycin MIC50/90 values were 8 and &gt;128 mg/L for CRKp isolates, which are much higher than previously reported. Twenty-four isolates (15.5%) were apramycin resistant (MIC ≥64 mg/L) and they were all from the K. pneumoniae ST258 background. The 24 apramycin-resistant K. pneumoniae ST258 strains belonged to six different capsular types and 91.7% of them harboured the apramycin resistance gene aac(3’)-IV. Sequencing analysis showed that different ST258 capsular type strains shared a common non-conjugative IncR plasmid, co-harbouring aac(3’)-IV and blaKPC. A novel IncR and IncX3 cointegrate plasmid, p59494-RX116.1, was also identified in an ST258 strain, demonstrating how apramycin resistance can be spread from a non-conjugative plasmid through cointegration.

Conclusions
We described a high apramycin resistance rate in clinical CRKp isolates in the New York/New Jersey region, mainly among the epidemic K. pneumoniae ST258 strains. The high resistance rate in an epidemic K. pneumoniae clone raises concern regarding the further optimization and development of apramycin and apramycin-like antibiotics.
Building a Life in Australia: Young Hazara Men and the Journey to ‘Adulthood’
Authors: Neve Amy 
Published: April 2021

AbstractYoung Hazara men who entered Australia as unaccompanied minors have insecure futures. While this insecurity of pathways and opportunities is in some ways similar to that of other young people in Australia, the challenges and opportunities that unaccompanied minors face as refugee youth mean that their experiences of growing-up also have unique characteristics. The journey to adult life is still shaped by the core dimensions of employment, education, housing, and family relationships. However, for the young Hazara men who participated in this study, they are also sites of disruption and adaption. Their experiences suggest a desire to fulfil these goals within social structures that actively prevent their achievement. Drawing on interviews and ethnographic fieldwork, this article investigates the challenges and strategies of young asylum seekers during a period in their lives in which the transition to adult life intersects with the process of settlement. This research contributes to the growing literature that represents experiences of young adults in the wake of global diversity and social change.
Efficacy of one-dose intramuscular rabies vaccine as pre-exposure prophylaxis in travellers
Authors: Mills Deborah J , Lau Colleen L , Mills Christine , Furuya-Kanamori Luis 
Published: April 2021

Abstract

Background
Current guidelines for rabies pre-exposure prophylaxis (PrEP) recommend multiple vaccine doses. Travellers sometimes present for pre-travel consultation with insufficient time to complete standard PrEP schedules. We investigated the efficacy of one-dose intramuscular (IM) vaccine in priming the immune system (as PrEP) by measuring antibody response to simulated post-exposure prophylaxis (PEP).

Methods
A quasi-experimental pre–post intervention clinical trial was conducted at a specialist travel clinic in Australia. Adults (≥18 years) without a history of rabies vaccination were included. At Visit 1, seronegative status was confirmed and one dose of 0.5 ml IM rabies vaccine (Verorab®) administered. At Visit 2 (≥60 days after Visit 1), serology was repeated and a simulated PEP dose (0.5 ml IM) given on this day and again 3 days later (Visit 3). Serology was repeated at Visit 4 (7 days after Visit 2).

Results
A total of 94 antibody-negative participants were included (&lt;50 years [n = 50]; ≥50 years [n = 44]). At Visit 2, 38.0 and 31.8% of participants aged &lt;50 and ≥50 years were antibody-positive (≥0.5 EU/ml). At Visit 4, all participants were antibody-positive; 82.0 and 47.7% of participants aged &lt;50 and ≥50 years had antibody levels &gt;4 EU/ml, respectively.

Conclusions
One-dose IM vaccine was effective as PrEP for priming the immune system in both age groups, resulting in rapid development of antibodies 7 days after commencing simulated PEP. If there is insufficient time to complete a standard PrEP schedule, one-dose IM could be considered as an alternative schedule for short trips, rather than not offering travellers any doses at all.
Clinical trials registration: ACTRN12619000946112.
Between Love and Misogamy: Changing Perceptions of Marriage among German Jews, 1750–1800
Authors: Naimark-Goldberg Natalie 
Published: April 2021

Abstract
Marriage is a central and binding institution of Jewish life. However, as a historical construct, it was never a static, immutable structure. This article focuses on the changing attitudes towards marriage among German Jews in the second half of the eighteenth century. It discusses how rational considerations external to the couple’s personal needs and desires started losing ground, while its function as a framework for emotional and erotic satisfaction intensified. As marriage was increasingly perceived in terms of self-fulfilment, many pursued happiness through matrimony, embracing the new idea of the love marriage. Although this idea developed from contemporary trends in non-Jewish society, maskilic authors used Jewish sources to maintain this position, trying to present it as consistent with tradition rather than as a break from it. The emergence of a romantic discourse was not the only transformation in the perception of marriage. The individualism that impelled the notion of a love marriage led to another type of discourse among Jewish women and men: the discourse against marriage. Using the perspectives of continuity and change, the article seeks to discern the role that Judaism and Jewish sources played in discourses about misogamy and the modernization of the traditional institution of marriage.