bluuebunny/crossref_metadata_2025_split huggingface dataset:
Streptomyces venezuelae NRRL B-65442: genome sequence of a model strain used to study morphological differentiation in filamentous actinobacteria
Authors: Gomez-Escribano Juan Pablo , Holmes Neil A , Schlimpert Susan , Bibb Maureen J , Chandra Govind , Wilkinson Barrie , Buttner Mark J , Bibb Mervyn J 
Published: June 2021

Abstract
For over a decade, Streptomyces venezuelae has been used to study the molecular mechanisms that control morphological development in streptomycetes and is now a well-established model strain. Its rapid growth and ability to sporulate in a near-synchronised manner in liquid culture, unusual among streptomycetes, greatly facilitates the application of modern molecular techniques such as ChIP-seq and RNA-seq, as well as time-lapse fluorescence imaging of the complete Streptomyces life cycle. Here we describe a high-quality genome sequence of our isolate of the strain (Northern Regional Research Laboratory [NRRL] B-65442) consisting of an 8.2 Mb chromosome and a 158 kb plasmid, pSVJI1, which had not been reported previously. Surprisingly, while NRRL B-65442 yields green spores on MYM agar, the American Type Culture Collection (ATCC) type strain 10712 (from which NRRL B-65442 was derived) produces grey spores. While comparison of the genome sequences of the two isolates revealed almost total identity, it did reveal a single nucleotide substitution in a gene, vnz_33525, involved in spore pigment biosynthesis. Replacement of the vnz_33525 allele of ATCC 10712 with that of NRRL B-65442 resulted in green spores, explaining the discrepancy in spore pigmentation. We also applied CRISPR-Cas9 to delete the essential parB of pSVJI1 to cure the plasmid from the strain without obvious phenotypic consequences.
Side Effects 15 Years After Lymph Node Irradiation in Breast Cancer: Randomized EORTC Trial 22922/10925
Authors: Poortmans Philip M , Struikmans Henk , De Brouwer Peter , Weltens Caroline , Fortpied Catherine , Kirkove Carine , Budach Volker , Peignaux-Casasnovas Karine , van der Leij Femke , Vonk Ernest , Valli Mariacarla , vanTienhoven Geertjan , Weidner Nicola , Noel Georges , Guckenberger Matthias , Koiter Eveline , vanLimbergen Erik , Engelen Antoine , Fourquet Alain , Bartelink Harry ,   for the EORTC Radiation Oncology and Breast Cancer Groups
Published: June 2021

Abstract

Background
Uncertainty about the benefit–risk ratio of regional lymph node irradiation led to varying clinical protocols. We investigated long-term late side effects after internal mammary and medial supraclavicular (IM-MS) lymph node irradiation to improve shared decision making.

Methods
The multicenter European Organization for Research and Treatment of Cancer trial (ClinicalTrials.gov, NCT00002851) randomly assigned stage I-III breast cancer patients with involved axillary nodes and/or a medially located primary tumor. We analyzed late side effects both longitudinally at every follow-up and cross-sectionally at 5-year intervals. All statistical tests were 2-sided.

Results
Between 1996 and 2004, 46 departments from 13 countries accrued 4004 patients. Median follow-up was 15.7 years. Longitudinal follow-up data showed cumulative incidence rates at 15 years of 2.9% (95% confidence interval [CI] = 2.2% to 3.8%) vs 5.7% (95% CI = 4.7% to 6.9%) (P &lt; .001) for lung fibrosis, 1.1% (95% CI = 0.7% to 1.7%) vs 1.9% (95% CI = 1.3% to 2.6%) (P = .07) for cardiac fibrosis, and 9.4% (95% CI = 8.0% to 10.8%) vs 11.1% (95% CI = 9.6% to 12.7%) (P = .04) for any cardiac disease when treated without or with IM-MS lymph node irradiation. There was no evidence for differences between left- and right-sided breast cancer (Wald χ2 test of treatment by breast side interaction, P = .33 and P = .35, for cardiac fibrosis and for any cardiac disease, respectively). The cumulative incidence probabilities of cross-sectionally reported side effects with a score of 2 or greater at 15 years were 0.1% (95% CI = 0.0% to 0.5%) vs 0.8% (95% CI = 0.4% to 1.4%) for pulmonary (P = .02), 1.8% (95% CI = 1.1% to 2.8%) vs 2.6% (95% CI = 1.8% to 3.7%) for cardiac (P = .15), and 0.0% (95% CI not evaluated) vs 0.1% (95% CI = 0.0% to 0.4%) for esophageal (P = .16), respectively. No difference was observed in the incidence of second malignancies, contralateral breast cancer, or cardiovascular deaths.

Conclusions
The incidence of late pulmonary side effects was statistically significantly higher after IM-MS lymph node irradiation, as were some of the cardiac events, without a difference between left- and right-sided treatments. Absolute rates and differences were very low, without increased non-breast cancer–related mortality, even before introducing heart-sparing techniques.
Reduced expression of lipoxygenase genes improves flour processing quality in soft wheat
Authors: Lv Guoguo , Tian Qiuzhen , Zhang Fuyan , Chen Jianhui , Niaz Mohsin , Liu Chunyi , Hu Huiting , Sun Congwei , Chen Feng 
Published: June 2021

Abstract
Lipoxygenases (Loxs) are dioxygenases that play an important role in plant growth and defense. Loxs affect flour processing quality in common wheat (Triticum aestivum). We conducted a genome-wide association study (GWAS) that identified 306 significant single-nucleotide polymorphisms (SNPs) related to Lox activity in Chinese wheat accessions. Among them, a novel lipoxygenase-encoding (Lpx) gene, TaLpx-B4, was detected on chromosome 3B in a biparental population. Analysis of mutant wheat lines induced using ethyl methanesulfonate confirmed the role of TaLpx-B4 in modulating Lox activity. A phylogenetic tree of various plant Lpx genes indicated the predominance of the 9-Lpx type in common wheat. Further analysis revealed conserved intron number, exon length, and motif number in the TaLpx gene family. GWAS, linkage mapping, and gene annotation collectively showed that 14 out of 29 annotated TaLpx genes played a critical role in regulating Lox activity in the Chinese wheat accessions. Transgenic wheat grains with knockdown of Lpx family genes by RNAi showed significantly lower Lox activity than the wild type. One TaLpx-RNAi line had significantly reduced starch content and dough stability, and thus possessed relatively superior biscuit quality in soft wheat. Further analysis of the transcriptome, lipid components, and other metabolites revealed that knockdown of TaLpx genes significantly increased biscuit quality via changes in unsaturated fatty acid content as well as in starch, sucrose, and galactose metabolism. Our results provide new insights into the role of the TaLpx gene family that will be beneficial in improving soft wheat flour quality.
Stress effects on the reactive oxygen species-dependent regulation of plant growth and development
Authors: Considine Michael J , Foyer Christine H 
Published: June 2021

Abstract
Plant growth is mediated by cell proliferation and expansion. Both processes are controlled by a network of endogenous factors such as phytohormones, reactive oxygen species (ROS), sugars, and other signals, which influence gene expression and post-translational regulation of proteins. Stress resilience requires rapid and appropriate responses in plant growth and development as well as defence. Regulation of ROS accumulation in different cellular compartments influences growth responses to abiotic and biotic stresses. While ROS are essential for growth, they are also implicated in the stress-induced cessation of growth and, in some cases, programmed cell death. It is widely accepted that redox post-translational modifications of key proteins determine the growth changes and cell fate responses to stress, but the molecular pathways and factors involved remain poorly characterized. Here we discuss ROS as a signalling molecule, the mechanisms of ROS-dependent regulation that influence protein–protein interactions, protein function, and turnover, together with the relocation of key proteins to different intracellular compartments in a manner that can alter cell fate. Understanding how the redox interactome responds to stress-induced increases in ROS may provide a road map to tailoring the dynamic ROS interactions that determine growth and cell fate in order to enhance stress resilience.
The temperatures of red supergiants in low-metallicity environments
Authors: González-Torà Gemma , Davies Ben , Kudritzki Rolf-Peter , Plez Bertrand 
Published: June 2021

ABSTRACT
The temperatures of red supergiants (RSGs) are expected to depend on metallicity (Z) in such a way that lower Z RSGs are warmer. In this work, we investigate the Z-dependence of the Hayashi limit by analysing RSGs in the low-Z galaxy Wolf–Lundmark–Mellote, and compare with the RSGs in the higher Z environments of the Small Magellanic Cloud and Large Magellanic Cloud. We determine the effective temperature (Teff) of each star by fitting their spectral energy distributions, as observed by VLT + SHOOTER, with MARCS model atmospheres. We find average temperatures of $T_{\textrm {eff}_{\textrm {WLM}}}=4400\pm 202$ K, $T_{\textrm {eff}_{\textrm {SMC}}}=4130\pm 103$ K, and $T_{\textrm {eff}_{\textrm {LMC}}}=4140\pm 148$ K. From population synthesis analysis, we find that although the Geneva evolutionary models reproduce this trend qualitatively, the RSGs in these models are systematically too cool. We speculate that our results can be explained by the inapplicability of the standard solar mixing length to RSGs.
An Expert Elicitation on the Effects of a Ban on Menthol Cigarettes and Cigars in the United States
Authors: Levy David T , Cadham Christopher J , Sanchez-Romero Luz Maria , Knoll Marie , Travis Nargiz , Yuan Zhe , Li Yameng , Mistry Ritesh , Douglas Clifford E , Tam Jamie , Sertkaya Aylin , Warner Kenneth E , Meza Rafael 
Published: June 2021

AbstractIntroductionThe US Food and Drug Administration announced its intention to ban menthol in cigarettes. However, information is needed on how a federal ban would affect population health.Aims and MethodsWe conducted an expert elicitation to gauge the impact of a menthol cigarette and cigar ban in the United States. We developed and pilot tested a questionnaire that focused on tobacco use transitions of current smokers (age 18–24 menthol, age 35–54 menthol, and age 35–54 nonmenthol) and potential menthol smokers (age 12–24). Using a structured expert elicitation, we estimated mean net transitions under a ban from cigarette use to combustible tobacco product, smokeless tobacco, novel nicotine delivery product (NNDPs, such as e-cigarettes) use, or no tobacco use.ResultsEleven experts provided responses. Of those ages 12–24 who would have initiated menthol cigarette use in the absence of a ban, the experts estimated that 41% would still initiate combustible products under a ban, while 18% would initiate with NNDPs and 39% would not initiate regular tobacco use. Combustible use by menthol smokers ages 35–54 was expected to decline by 20% postban relative to preban rates, half switching to NNDPs and half quitting all tobacco use. Menthol smokers ages 18–24 were expected to reduce combustible use by 30%, with 16% switching to NNDPs. Greater reductions in combustible use were estimated for African Americans across the three age groups. Negligible impacts were expected for current adult nonmenthol smokers.ConclusionsAccording to expert opinion, a menthol ban is expected to substantially reduce smoking initiation and combustible tobacco product use among current menthol smokers.ImplicationsThe US Food and Drug Administration recently announced its intention to ban menthol in cigarettes, but information on the potential impact on smoking and other nicotine product use is limited. We conducted an expert elicitation to gauge the impact of a menthol cigarette and cigar ban in the United States. A panel of experts estimated that menthol smokers ages 35–54 would reduce combustible tobacco use by 20%, with half switching to e-cigarettes and half quitting all nicotine use. Larger reductions were expected at younger ages, and menthol smoking initiation was reduced by 59% with 18% instead using e-cigarettes. African Americans were expected to have greater reductions in combustible tobacco use than the rest of the population.
Effect of the Equity Capital Ratio on the Relationship between Competition and Bank Risk-Taking Behavior
Authors: Hao Jia , Zheng Kuncheng 
Published: June 2021

AbstractWe examine how the relationship between competition and risk-taking changes with the ex ante bank equity capital ratio. We show that competition in the banking market, on average, mitigates risk-taking by banks. This relationship, however, can be altered by a bank’s ex ante equity capital ratio. More specifically, when face with increased competition, banks with low ex ante equity capital ratios engage in relatively larger reductions in risk-taking. They do so primarily by decreasing the risk in their lending portfolios. In contrast, banks with high enough ex ante equity capital ratios might not reduce their risk-taking at all. (JEL G21, G32, O16, D40, G18)
Morphological characteristics of sacroiliac joint MRI lesions in axial spondyloarthritis and control subjects
Authors: Seven Sengül , Østergaard Mikkel , Morsel-Carlsen Lone , Sørensen Inge Juul , Bonde Birthe , Thamsborg Gorm , Lykkegaard Jens Jørgen , Pedersen Susanne Juhl 
Published: June 2021

Abstract

Objectives
To investigate SI joint MRI inflammation, structural and degenerative lesion characteristics in patients with axial spondyloarthritis (axSpA) and various control groups.

Methods
Patients with axSpA (n = 41) and lumbar disc herniation (n = 25), women with (n = 46) and without (n = 14) post-partum (childbirth within 4–16 months) buttock/pelvic pain, cleaning assistants (n = 26), long-distance runners (n = 23) and healthy men (n = 29) had MRI of the SI joints prospectively performed. MRI lesions were assessed on nine slices covering the cartilaginous compartment by two experienced readers according to the definitions of the Spondyloarthritis Research Consortium of Canada SI joint inflammation and structural scores, and were evaluated according to depth and extent. Other morphological characteristics were also analysed.

Results
Total depth scores for bone marrow oedema (BME) and fat lesion (FAT) and total extent score for erosion were statistically significantly highest in axSpA, while scores for sclerosis were numerically highest in women with post-partum pain. Maximum BME depth &gt;10 mm was frequently and exclusively found in axSpA and post-partum women (39% vs 14–17%) while FAT depth &gt;5 mm was predominantly found in axSpA (76% vs 0–10%). Erosions were primarily seen in axSpA, especially when extensive (≥4 or confluent; 17% vs 0%). Capsulitis was absent in non-axSpA groups. BME and FAT in the ligamentous compartment were primarily found in axSpA (17/22% vs 0/2% in non-axSpA groups). In non-axSpA, osteophytes (axSpA vs non-axSpA: 0% vs 3–17%) and vacuum phenomenon (7% vs 30–66%) were more frequent, and the joint space was wider [mean (s.d.) 1.5 (0.9) vs 2.2 (0.5) mm].

Conclusions
FAT depth &gt;5 mm, but not BME depth &gt;10 mm, could almost differentiate axSpA patients from all other groups. When excluding post-partum women, BME &gt;5 mm and erosion were highly specific for axSpA.
Financial oikonomization: the financial government and administration of the household
Authors: Ossandón José , Deville Joe , Lazarus Jeanne , Luzzi Mariana 
Published: June 2021

AbstractThis article introduces a new social scientific understanding of the relationship between households and finance. We call it financial oikonomization. Financial oikonomization signals a specific research problem and a distinctive analytical approach to this problem. The problem is how households are financially administered and governed. The approach is pragmatist and descriptive. It is oriented to the how, to the study of the problems and practices of those directly involved with administrating and governing households’ financial flows. On the basis of an extensive review of recent research, we distinguish seven operations of financial oikonomization, seven distinct problems—with their own practices, sites and techniques, but all oriented to the financial administration and government of households. We call these operations: budgeting, juggling, evaluating, attaching, educating, publicizing and infrastructuring.
Accelerated Diversification Explains the Exceptional Species Richness of Tropical Characoid Fishes
Authors: Melo Bruno F , Sidlauskas Brian L , Near Thomas J , Roxo Fabio F , Ghezelayagh Ava , Ochoa Luz E , Stiassny Melanie L J , Arroyave Jairo , Chang Jonathan , Faircloth Brant C , MacGuigan Daniel J , Harrington Richard C , Benine Ricardo C , Burns Michael D , Hoekzema Kendra , Sanches Natalia C , Maldonado-Ocampo Javier A , Castro Ricardo M C , Foresti Fausto , Alfaro Michael E , Oliveira Claudio 
Published: June 2021

AbstractThe Neotropics harbor the most species-rich freshwater fish fauna on the planet, but the timing of that exceptional diversification remains unclear. Did the Neotropics accumulate species steadily throughout their long history, or attain their remarkable diversity recently? Biologists have long debated the relative support for these museum and cradle hypotheses, but few phylogenies of megadiverse tropical clades have included sufficient taxa to distinguish between them. We used 1288 ultraconserved element loci spanning 293 species, 211 genera, and 21 families of characoid fishes to reconstruct a new, fossil-calibrated phylogeny and infer the most likely diversification scenario for a clade that includes a third of Neotropical fish diversity. This phylogeny implies paraphyly of the traditional delimitation of Characiformes because it resolves the largely Neotropical Characoidei as the sister lineage of Siluriformes (catfishes), rather than the African Citharinodei. Time-calibrated phylogenies indicate an ancient origin of major characoid lineages and reveal a much more recent emergence of most characoid species. Diversification rate analyses infer increased speciation and decreased extinction rates during the Oligocene at around 30 Ma during a period of mega-wetland formation in the proto-Orinoco-Amazonas. Three species-rich and ecomorphologically diverse lineages (Anostomidae, Serrasalmidae, and Characidae) that originated more than 60 Ma in the Paleocene experienced particularly notable bursts of Oligocene diversification and now account collectively for 68% of the approximately 2150 species of Characoidei. In addition to paleogeographic changes, we discuss potential accelerants of diversification in these three lineages. While the Neotropics accumulated a museum of ecomorphologically diverse characoid lineages long ago, this geologically dynamic region also cradled a much more recent birth of remarkable species-level diversity. [Biodiversity; Characiformes; macroevolution; Neotropics; phylogenomics; ultraconserved elements.]
In Defence of AIB v Mark Redler
Authors: Chan Alex 
Published: June 2021

Abstract
Following Target Holdings v Redferns and AIB v Mark Redler, it is now common theme that equitable compensation is underpinned by a compensatory mindset requiring but-for causation. This article argues that, despite criticisms that the compensatory reasoning does not sit well with the orthodoxy, the law has moved on. The traditional accounting mechanism should no longer be maintained since it could create unjust results. The better approach is to look at the obligation in question which, together with the underlying policy and the remedial goal, informs the proper remedy to a particular claim.
Feeding different cultivars and quality levels of faba bean to broiler chickens
Authors: Smit Miranda N , He Liangfei , Beltranena Eduardo 
Published: June 2021

Abstract
A concern of both pulse growers and poultry producers is how frost damage around harvest time affects the nutritional quality of faba bean for broiler chickens. To investigate, two zero-tannin cultivars (Snowbird, Snowdrop) and one low vicine and convicine cultivar (Fabelle) sourced from seed growers were spring planted 3 weeks later than recommended (mid-May) and harvested late October to purposely increase frost damage. Parent, certified seed (high quality), and harvested frost damaged beans (low quality) of the three cultivars were fed to 740 chickens housed in 64 floor pens in a 2 × 3 factorial plus control (9 pens of 11 or 12 birds per treatment). Starter (d 0 to 11), grower (d 12 to 24), and finisher (d 25 to 40) diets included 15%, 30%, and 45% faba bean in partial (starter, grower) or total replacement of soybean meal (SBM; control). Harvested Snowbird, Snowdrop, Fabelle averaged 52%, 62%, 17% blackened hull and 35%, 43%, 51% immature beans, respectively. There was a cultivar × quality interaction (P &lt; 0.05) on daily feed disappearance (ADFI) and gain-to-feed (G:F). Broilers fed low-quality Snowdrop consumed 10 g/d more finisher and 6 g/d more feed overall than those fed low-quality Snowbird or Fabelle; broilers fed parent seed were intermediate. Feeding low-quality Fabelle resulted in best overall G:F (0.646) versus high-quality Snowbird (0.611), high-quality Fabelle (0.624), or low-quality Snowdrop (0.624). Average daily weight gain (ADG) and bird body weight (BW) at the end of each growth phase were not affected by cultivar or quality level. Controls fed SBM only grew 2.75 g/d faster overall and were 113.5 g heavier at the end of the trial than broilers fed faba bean (P &lt; 0.05). Controls fed SBM only had 0.024 g/g better overall G:F than broilers fed faba bean (P &lt; 0.05). Feeding low-quality beans or high-quality seed had no effect on antemortem BW, chilled carcass weight (WT), dressing percentage or yield of saleable cuts except that broilers fed Snowbird or Snowdrop had 0.8%-unit larger thighs than those fed Fabelle. Controls fed SBM only were 110 g heavier at slaughter, had 72 g heavier chilled carcass WT, and 0.5%-unit greater dressing percentage than broilers fed faba bean (P &lt; 0.05). These results indicate that feeding frost damaged and(or) immature faba bean, to the extent observed in this trial, did not negatively affect growth performance or carcass attributes of broiler chickens compared to feeding parent, certified, high-quality seed of these cultivars.
Increasing Advance Care Planning Conversations Among Korean Americans Through a Faith Community Nurse Intervention
Authors: Park Morgan 
Published: June 2021

ABSTRACT:
Minority populations have lower rates of advance care planning (ACP) than the general population. Faith community nurses (FCNs) can facilitate ACP training to improve end-of-life care outcomes for patients and caregivers. A nurse-led ACP training delivered to a primarily Korean American faith community increased the number of families who discussed end-of-life care values and wishes. Considerations for ACP outreach to Korean Americans and implications for FCNs are discussed.