bluuebunny/crossref_metadata_2025_split huggingface dataset:
Recent trends in smartphone-based detection for biomedical applications: a review
Authors: Banik Soumyabrata , Melanthota Sindhoora Kaniyala , Arbaaz  , Vaz Joel Markus , Kadambalithaya Vishak Madhwaraj , Hussain Iftak , Dutta Sibasish , Mazumder Nirmal 
Published: February 2021

AbstractSmartphone-based imaging devices (SIDs) have shown to be versatile and have a wide range of biomedical applications. With the increasing demand for high-quality medical services, technological interventions such as portable devices that can be used in remote and resource-less conditions and have an impact on quantity and quality of care. Additionally, smartphone-based devices have shown their application in the field of teleimaging, food technology, education, etc. Depending on the application and imaging capability required, the optical arrangement of the SID varies which enables them to be used in multiple setups like bright-field, fluorescence, dark-field, and multiple arrays with certain changes in their optics and illumination. This comprehensive review discusses the numerous applications and development of SIDs towards histopathological examination, detection of bacteria and viruses, food technology, and routine diagnosis. Smartphone-based devices are complemented with deep learning methods to further increase the efficiency of the devices.
A Noise-Induced Transition in the Lorenz System
Authors: Coti Zelati Michele , Hairer Martin 
Published: February 2021

AbstractWe consider a stochastic perturbation of the classical Lorenz system in the range of parameters for which the origin is the global attractor. We show that adding noise in the last component causes a transition from a unique to exactly two ergodic invariant measures. The bifurcation threshold depends on the strength of the noise: if the noise is weak, the only invariant measure is Gaussian, while strong enough noise causes the appearance of a second ergodic invariant measure.
Homological branching law for $$({\mathrm {GL}}_{n+1}(F), {\mathrm {GL}}_n(F))$$: projectivity and indecomposability
Authors: Chan Kei Yuen 
Published: February 2021

AbstractLetFbe a non-Archimedean local field. This paper studies homological properties of irreducible smooth representations restricted from$${\mathrm {GL}}_{n+1}(F)$$GLn+1(F)to$${\mathrm {GL}}_n(F)$$GLn(F). A main result shows that each Bernstein component of an irreducible smooth representation of$${\mathrm {GL}}_{n+1}(F)$$GLn+1(F)restricted to$${\mathrm {GL}}_n(F)$$GLn(F)is indecomposable. We also classify all irreducible representations which are projective when restricting from$${\mathrm {GL}}_{n+1}(F)$$GLn+1(F)to$${\mathrm {GL}}_n(F)$$GLn(F). A main tool of our study is a notion of left and right derivatives, extending some previous work joint with Gordan Savin. As a by-product, we also determine the branching law in the opposite direction.
Antibacterial and safety tests of a flexible cold atmospheric plasma device for the stimulation of wound healing
Authors: Boekema Bouke , Stoop Matthea , Vlig Marcel , van Liempt Jos , Sobota Ana , Ulrich Magda , Middelkoop Esther 
Published: February 2021

Abstract
Cold atmospheric plasma (CAP) devices generate an ionized gas with highly reactive species and electric fields at ambient air pressure and temperature. A flexible dielectric barrier discharge (DBD) was developed as an alternative antimicrobial treatment for chronic wounds. Treatment of Staphylococcus aureus in collagen-elastin matrices with CAP for 2 min resulted in a 4 log reduction. CAP treatment was less effective on S. aureus on dermal samples. CAP did not affect cellular activity or DNA integrity of human dermal samples when used for up to 2 min. Repeated daily CAP treatments for 2 min lowered cellular activity of dermal samples to 80% after 2 to 4 days, but this was not significant. Repeated treatment of ex vivo human burn wound models with CAP for 2 min did not affect re-epithelialization. Intact skin of 25 healthy volunteers was treated with CAP for 3× 20” to determine safety. Although participants reported moderate pain scores (numerical rating scale 3.3), all volunteers considered the procedure to be acceptable. Severe adverse events did not occur. CAP treatment resulted in a temporarily increased local skin temperature (≈3.4°C) and increased erythema. Lowering the plasma power resulted in a significantly lower erythema increase. Good log reduction (2.9) of bacterial load was reached in 14/15 volunteers artificially contaminated with Pseudomonas aeruginosa. This study demonstrated the in vitro and in vivo safety and efficacy in bacterial reduction of a flexible cold plasma device. Trial registration number NCT03007264, January 2, 2017

Key Points
• CAP strongly reduced bacterial numbers both in vitro and in vivo.
• Re-epithelialization of burn wound models was not affected by repeated CAP.
• CAP treatment of intact skin was well tolerated in volunteers.
Transpathology: molecular imaging-based pathology
Authors: Tian Mei , He Xuexin , Jin Chentao , He Xiao , Wu Shuang , Zhou Rui , Zhang Xiaohui , Zhang Kai , Gu Weizhong , Wang Jing , Zhang Hong 
Published: February 2021

AbstractPathology is the medical specialty concerned with the study of the disease nature and causes, playing a key role in bridging basic researches and clinical medicine. In the course of development, pathology has significantly expanded our understanding of disease, and exerted enormous impact on the management of patients. However, challenges facing pathology, the inherent invasiveness of pathological practice and the persistent concerns on the sample representativeness, constitute its limitations. Molecular imaging is a noninvasive technique to visualize, characterize, and measure biological processes at the molecular level in living subjects. With the continuous development of equipment and probes, molecular imaging has enabled an increasingly precise evaluation of pathophysiological changes. A new pathophysiology visualization system based on molecular imaging is forming and shows the great potential to reform the pathological practice. Several improvements in “trans-,” including trans-scale, transparency, and translation, would be driven by this new kind of pathological practice. Pathological changes could be evaluated in a trans-scale imaging mode; tissues could be transparentized to better present the underlying pathophysiological information; and the translational processes of basic research to the clinical practice would be better facilitated. Thus, transpathology would greatly facilitate in deciphering the pathophysiological events in a multiscale perspective, and supporting the precision medicine in the future.
Two muscular variations in the elbow associated with the anterior interosseous nerve
Authors: Olewnik Łukasz , Szewczyk Bartłomiej , Zielinska Nicol , Grzelecki Dariusz , Polguj Michał 
Published: February 2021

AbstractThe coexistence of different muscular-neurovascular variations is of significant clinical importance. A male cadaver, 76 years old at death, was subjected to routine anatomical dissection; the procedure was performed for research and teaching purposes at the Department of Anatomical Dissection and Donation, Medical University of Lodz. The right forearm and hand were dissected using standard techniques according to a strictly specified protocol. The presence accessory head of the flexor pollicis longus may potentially compress the anterior interosseous nerve. The present case report describes a rare variant of the ulnar head of the pronator teres, characterized by two independent bands (i.e., two proximal attachments). The main band originates from the coronoid process and the second originates from the tendon of the biceps brachii. This type of attachment could potentially affect the compression of the ulnar artery running between the two bands. Additionally, the accessory head of the flexor pollicis longus was observed, which started on the medial epicondyle; its coexistence with a high division median nerve creates a potential pressure site on the anterior interesosseous nerve.
Information geometry for phylogenetic trees
Authors: Garba M. K. , Nye T. M. W. , Lueg J. , Huckemann S. F. 
Published: February 2021

AbstractWe propose a new space of phylogenetic trees which we callwald space. The motivation is to develop a space suitable for statistical analysis of phylogenies, but with a geometry based on more biologically principled assumptions than existing spaces: in wald space, trees are close if they induce similar distributions on genetic sequence data. As a point set, wald space contains the previously developed Billera–Holmes–Vogtmann (BHV) tree space; it also contains disconnected forests, like the edge-product (EP) space but without certain singularities of the EP space. We investigate two related geometries on wald space. The first is the geometry of the Fisher information metric of character distributions induced by the two-state symmetric Markov substitution process on each tree. Infinitesimally, the metric is proportional to the Kullback–Leibler divergence, or equivalently, as we show, to anyf-divergence. The second geometry is obtained analogously but using a related continuous-valued Gaussian process on each tree, and it can be viewed as the trace metric of the affine-invariant metric for covariance matrices. We derive a gradient descent algorithm to project from the ambient space of covariance matrices to wald space. For both geometries we derive computational methods to compute geodesics in polynomial time and show numerically that the two information geometries (discrete and continuous) are very similar. In particular, geodesics are approximated extrinsically. Comparison with the BHV geometry shows that our canonical and biologically motivated space is substantially different.
Growth impacts in a changing ocean: insights from two coral reef fishes in an extreme environment
Authors: D’Agostino Daniele , Burt John A. , Santinelli Veronica , Vaughan Grace O. , Fowler Ashley M. , Reader Tom , Taylor Brett M. , Hoey Andrew S. , Cavalcante Geórgenes H. , Bauman Andrew G. , Feary David A. 
Published: February 2021

AbstractDetermining the life-history consequences for fishes living in extreme and variable environments will be vital in predicting the likely impacts of ongoing climate change on reef fish demography. Here, we compare size-at-age and maximum body size of two common reef fish species (Lutjanus ehrenbergii and Pomacanthus maculosus) between the environmentally extreme Arabian/Persian Gulf (‘Arabian Gulf’) and adjacent comparably benign Oman Sea. Additionally, we use otolith increment width profiles to investigate the influence of temperature, salinity and productivity on the individual growth rates. Individuals of both species showed smaller size-at-age and lower maximum size in the Arabian Gulf compared to conspecifics in the less extreme and less variable environment of the Oman Sea, suggesting a life-history trade-off between size and metabolic demands. Salinity was the best environmental predictor of interannual growth across species and regions, with low growth corresponding to more saline conditions. However, salinity had a weaker negative effect on interannual growth of fishes in the Arabian Gulf than in the Oman Sea, indicating Arabian Gulf populations may be better able to acclimate to changing environmental conditions. Temperature had a weak positive effect on the interannual growth of fishes in the Arabian Gulf, suggesting that these populations may still be living within their thermal windows. Our results highlight the potential importance of osmoregulatory cost in impacting growth, and the need to consider the effect of multiple stressors when investigating the consequences of future climate change on fish demography.
North Atlantic gyre circulation in PRIMAVERA models
Authors: Meccia Virna L. , Iovino Doroteaciro , Bellucci Alessio 
Published: February 2021

AbstractWe study the impact of horizontal resolution in setting the North Atlantic gyre circulation and representing the ocean–atmosphere interactions that modulate the low-frequency variability in the region. Simulations from five state-of-the-art climate models performed at standard and high-resolution as part of the High-Resolution Model Inter-comparison Project (HighResMIP) were analysed. In some models, the resolution is enhanced in the atmospheric and oceanic components whereas, in some other models, the resolution is increased only in the atmosphere. Enhancing the horizontal resolution from non-eddy to eddy-permitting ocean produces stronger barotropic mass transports inside the subpolar and subtropical gyres. The first mode of inter-annual variability is associated with the North Atlantic Oscillation (NAO) in all the cases. The rapid ocean response to it consists of a shift in the position of the inter-gyre zone and it is better captured by the non-eddy models. The delayed ocean response consists of an intensification of the subpolar gyre (SPG) after around 3 years of a positive phase of NAO and it is better represented by the eddy-permitting oceans. A lagged relationship between the intensity of the SPG and the Atlantic Meridional Overturning Circulation (AMOC) is stronger in the cases of the non-eddy ocean. Then, the SPG is more tightly coupled to the AMOC in low-resolution models.
CaMKII and PKA-dependent phosphorylation co-regulate nuclear localization of HDAC4 in adult cardiomyocytes
Authors: Helmstadter Kathryn G. , Ljubojevic-Holzer Senka , Wood Brent M. , Taheri Khanha D. , Sedej Simon , Erickson Jeffrey R. , Bossuyt Julie , Bers Donald M. 
Published: February 2021

AbstractNuclear histone deacetylase 4 (HDAC4) represses MEF2-mediated transcription, implicated in the development of heart failure. CaMKII-dependent phosphorylation drives nucleus-to-cytoplasm HDAC4 shuttling, but protein kinase A (PKA) is also linked to HDAC4 translocation. However, the interplay of CaMKII and PKA in regulating adult cardiomyocyte HDAC4 translocation is unclear. Here we sought to determine the interplay of PKA- and CaMKII-dependent HDAC4 phosphorylation and translocation in adult mouse, rabbit and human ventricular myocytes. Confocal imaging and protein analyses revealed that inhibition of CaMKII—but not PKA, PKC or PKD—raised nucleo-to-cytoplasmic HDAC4 fluorescence ratio (FNuc/FCyto) by ~ 50%, indicating baseline CaMKII activity that limits HDAC4 nuclear localization. Further CaMKII activation (via increased extracellular [Ca2+], high pacing frequencies, angiotensin II or overexpression of CaM or CaMKIIδC) led to significant HDAC4 nuclear export. In contrast, PKA activation by isoproterenol or forskolin drove HDAC4 into the nucleus (raising FNuc/FCyto by > 60%). These PKA-mediated effects were abolished in cells pretreated with PKA inhibitors and in cells expressing mutant HDAC4 in S265/266A mutant. In physiological conditions where both kinases are active, PKA-dependent nuclear accumulation of HDAC4 was predominant in the very early response, while CaMKII-dependent HDAC4 export prevailed upon prolonged stimuli. This orchestrated co-regulation was shifted in failing cardiomyocytes, where CaMKII-dependent effects predominated over PKA-dependent response. Importantly, human cardiomyocytes showed similar CaMKII- and PKA-dependent HDAC4 shifts. Collectively, CaMKII limits nuclear localization of HDAC4, while PKA favors HDAC4 nuclear retention and S265/266 is essential for PKA-mediated regulation. These pathways thus compete in HDAC4 nuclear localization and transcriptional regulation in cardiac signaling.
Local bone quality measure and construct failure prediction: a biomechanical study on distal femur fractures
Authors: Gehweiler Dominic , Styger Ursula , Gueorguiev Boyko , Colcuc Christian , Vordemvenne Thomas , Wähnert Dirk 
Published: February 2021

Abstract
Introduction
The aim of this investigation was to better understand the differences in local bone quality at the distal femur and their correlation with biomechanical construct failure, with the intention to identify regions of importance to optimize implant anchorage.

Materials and methods
Seven fresh–frozen female femurs underwent high-resolution peripheral quantitative computed tomography (HR-pQCT) to determine bone mineral density (BMD) within three different regions of interest (distal, intermedium, and proximal) at the distal femur. In addition, local bone quality was assessed by measuring the peak torque necessary to break out the trabecular bone along each separate hole of a locking compression plate (LCP) during its instrumentation. Finally, biomechanical testing was performed using cyclic axial loading until failure in an AO/OTA 33 A3 fracture model.

Results
Local BMD was highest in the distal region. This was confirmed by the measurement of local bone quality using DensiProbe™. The most distal holes represented locations with the highest breakaway torque resistance, with the holes on the posterior side of the plate indicating higher values than those on its anterior side. We demonstrated strong correlation between the cycles to failure and local bone strength (measured with DensiProbe™) in the most distal posterior screw hole, having the highest peak torque.

Conclusion
The local bone quality at the distal femur indicates that in plated distal femur fractures the distal posterior screw holes seem to be the key ones and should be occupied. Measurement of the local bone strength with DensiProbe™ is one possibility to determine the risk of construct failure, therefore, thresholds need to be defined.
Cerebrovascular manifestations in hematological diseases: an update
Authors: Ferro José M. , Infante Joana 
Published: February 2021

AbstractPatients with hematological diseases often experience cerebrovascular complications including ischemic stroke, intracerebral and subarachnoid hemorrhage, microbleeds, posterior reversible encephalopathy syndrome, and dural sinus and cerebral vein thrombosis (CVT). In this update, we will review recent advances in the management of cerebrovascular diseases in the context of myeloproliferative neoplasms, leukemias, lymphomas, multiple myeloma, POEMS, paroxysmal nocturnal hemoglobinuria (PNH), thrombotic thrombocytopenic purpura (TTP), and sickle-cell disease. In acute ischemic stroke associated with hematological diseases, thrombectomy can in general be applied if there is a large vessel occlusion. Intravenous thrombolysis can be used in myeloproliferative neoplasms and sickle-cell anemia, but in other diseases, a case-by-case evaluation of the bleeding risks is mandatory. Patients with sickle-cell disease and acute stroke need very often to be transfused. In PNH, acute ischemic stroke patients must be anticoagulated. Most patients with CVT can be treated with low-molecular weight heparin (LMWH) acutely, even those with leukemias. Prevention of recurrence of cerebral thrombotic events depends on the control of the underlying disease, combined in some conditions with antithrombotic drugs. The recent introduction of specific monoclonal antibodies in the treatment of PHN and TTP has dramatically reduced the risk of arterial and venous thrombosis.
Profiles of endogenous ABA, bioactive GAs, IAA and their metabolites in Medicago truncatula Gaertn. non-embryogenic and embryogenic tissues during induction phase in relation to somatic embryo formation
Authors: Kępczyńska Ewa , Orłowska Anna 
Published: February 2021

Abstract
Main conclusion
During the 3-week-long induction phase, when M. truncatula cells leaf explants from non-embryogenic genotype (M9) and embryogenic variant (M9-10a) were forming the callus, biosynthesis and degradation of ABA, Gas and IAA proceeded at different levels. Induction of embryo formation is related to a lower ABA content, compared to the content of IAA and that of total bioactive GAs.

Abstract
Endogenous phytohormones are involved in the regulation of zygotic embryogenesis, but their role, especially of ABA, a plant growth inhibitor, in inducing somatic embryogenesis (SE) in angiosperms is still incompletely known. To arrive a better understanding of the ABA role in the process, we analyzed simultaneously and in detail changes in the contents of both ABA and five bioactive GAs (GA4, GA7, GA1, GA3, GA6) and IAA in M. truncatula non-embryogenic M9 (NE) and embryogenic M9-10a (E) genotypes. The initial leaf explants of both genotypes, and particularly NE, contained many times more ABA compared to the total bioactive GAs or IAA. In tissues during the entire 21-day induction all the hormones mentioned and their metabolites or conjugates were present; however, their contents were found to differ between the lines tested. The ABA level in primary explants of NE genotype was more than two times higher than that in E genotype. An even larger difference in the ABA content was found on the last day (day 21) of the induction phase (IP); the ABA content in E callus was over six times lower than in NE callus. In contrast, the IAA and GAs contents in primary explants of both genotypes in relation to ABA were low, but the contents of IAA and GAs exceeded that of ABA in the M9-10a tissues on the last day of IP. It is shown for the first time that endogenous ABA together with endogenous bioactive GAs and IAA is involved in acquisition of embryogenic competence in Medicago truncatula leaf somatic cells. These findings have a strong functional implication as they allow to improve the SE induction protocol.
Similar glycaemic control and risk of hypoglycaemia with patient- versus physician-managed titration of insulin glargine 300 U/mL across subgroups of patients with T2DM: a post hoc analysis of ITAS
Authors: Giaccari Andrea , Bonadonna R. C. , Buzzetti R. , Perseghin G. , Cucinotta D. , Fanelli C. , Avogaro A. , Aimaretti G. , Larosa M. , Pagano V. , Bolli G. B. 
Published: February 2021

Abstract
Aims
The Italian Titration Approach Study (ITAS) demonstrated comparable HbA1c reductions and similarly low hypoglycaemia risk at 6 months in poorly controlled, insulin-naïve adults with T2DM who initiated self- or physician-titrated insulin glargine 300 U/mL (Gla-300) in the absence of sulphonylurea/glinide. The association of patient characteristics with glycaemic and hypoglycaemic outcomes was assessed.

Methods
This post hoc analysis investigated whether baseline patient characteristics and previous antihyperglycaemic drugs were associated with HbA1c change and hypoglycaemia risk in patient- versus physician-managed Gla-300 titration.

Results
HbA1c change, incidence of hypoglycaemia (any type) and nocturnal rates were comparable between patient- and physician-managed arms in all subgroups. Hypoglycaemia rates across subgroups (0.03 to 3.52 events per patient-year) were generally as low as observed in the full ITAS population. Small increases in rates of 00:00–pre-breakfast and anytime hypoglycaemia were observed in the ≤ 10-year diabetes duration subgroup in the patient- versus physician-managed arm (heterogeneity of effect; p < 0.05).

Conclusions
Comparably fair glycaemic control and similarly low hypoglycaemia risk were achieved in almost all patient subgroups with patient- versus physician-led Gla-300 titration. These results reinforce efficacy and safety of Gla-300 self-titration across a range of phenotypes of insulin-naïve people with T2DM.

Clinical trial registration
EudraCT 2015-001167-39