bluuebunny/crossref_metadata_2025_split huggingface dataset:
Constrained evolutionary paths to macrolide resistance in aNeisseriacommensal converge on ribosomal genes through sequence duplication
Authors: Raisman Jordan C. , Fiore Michael A. , Tomin Lucille , Adjei Joseph K.O. , Aswad Virginia , Chu Jonathan , Domondon Christina J. , Donahue Ben A. , Masciotti Claudia A. , McGrath Connor G. , Melita Jo , Podbielski Paul A. , Schreiner Madelyn R. , Trumpore Lauren J. , Wengert Peter C. , Wrightstone Emalee A. , Hudson André O. , Wadsworth Crista B. 
Published: April 2021

AbstractNeisseriacommensals are an indisputable source of resistance for their pathogenic relatives; however, the evolutionary paths commensal species take to reduced susceptibility in this genus have been relatively underexplored. Here, we leveragein vitroselection as a powerful screen to identify the genetic adaptations that produce azithromycin resistance (≤ 2 μg/mL) in theNeisseriacommensal,N. elongata. Across multiple lineages (n=7/16), we find mutations encoding resistance converge on the gene encoding the 50S ribosomal L34 protein (rpmH) and the intergenic region proximal to the 30S ribosomal S3 protein (rpsC)through duplication events. Importantly, one of the laboratory evolved mutations inrpmHis identical, and two nearly identical, to those recently reported to confer high-level resistance to azithromycin inN. gonorrhoeae. Transformations into the ancestralN. elongatalineage confirmed the causality of bothrpmHandrpsCmutations. Though most lineages inheriting duplications sufferedin vitrofitness costs, one variant showed no growth defect, suggesting the possibility that it may be sustained in natural populations. Finally, we assessed the potential of horizontal transfer of derived resistance mutations into multiple strains ofN. gonorrhoeae. Though we were unable to transformN. gonorrhoeaein this case, studies like this will be critical for predicting commensal alleles that are at risk of rapid dissemination into pathogen populations.ImportanceCommensal bacterial populations have been increasingly recognized for their importance as sources of resistance for pathogens, however the collection of antimicrobial resistance (AMR) mechanisms within these communities are often understudied. The risk of reduced antibiotic susceptibility as a result of horizontal gene transfer (HGT) is amplified in highly recombinogenic genera, such as theNeisseria. Indeed, there have been multiple documented cases of macrolide and beta-lactam resistance acquisition in the pathogenN. gonorrhoeaefrom close commensal relatives. This work uncovers multiple novel azithromycin resistance-conferring mutations in aNeisseriacommensal through experimental evolution, investigates their fitness impacts, and explores the possibility of transfer toN. gonorrhoeae. Ultimately these types of studies will illuminate those resistance mutations that may rapidly be acquired across species boundaries.
Ripple Band Phase Precession of Place Cell Firing during Replay
Authors: Bush Daniel , Olafsdottir Freyja , Barry Caswell , Burgess Neil 
Published: April 2021

SummaryPhase coding offers several theoretical advantages for information transmission compared to an equivalent rate code. Phase coding is shown by place cells in the rodent hippocampal formation, which fire at progressively earlier phases of the movement related 6-12Hz theta rhythm as their spatial receptive fields are traversed. Importantly, however, phase coding is independent of carrier frequency, and so we asked whether it might also be exhibited by place cells during 150-250Hz ripple band activity, when they are thought to replay information to neocortex. We demonstrate that place cells which fire multiple spikes during candidate replay events do so at progressively earlier ripple phases, and that spikes fired across all replay events exhibit a negative relationship between decoded location within the firing field and ripple phase. These results provide insights into the mechanisms underlying phase coding and place cell replay, as well as the neural code propagated to downstream neurons.
Klebsiella pneumoniaeBolA contributes to cell morphology, siderophore production, stresses challenge, cell adhesion and virulence
Authors: Yan Xiangjin , Zhang Feiyang , Ding Manlin , Xiang Li , Bai Jiawei , Li Qin , Zhou Yingshun 
Published: April 2021

ABSTRACTKlebsiella pneumoniaeinfection is one of the important reasons for the increased of morbidity and mortality. The main virulence factors ofK. pneumoniaeinclude capsule polysaccharide, lipopolysaccharide, fimbriae, outer membrane proteins and siderophores. BolA homologues form a broadly conserved family of proteins in prokaryotes and eukaryotes. InEscherichia coli, bolAexpression is quickly induced in response to different stresses or stationary phase that rapidly adapt to changing environments. In this report, we confirmed thatbolAmutant strain exhibited increased sensitivity to bile and oxidative stresses. In addition, gene deletion showed thatbolAhas an important role for the adherence ofK. pneumoniaeto host cell and establishment in mice, including liver, spleen, kidney and lung tissues, and induce the formation of liver abscess in mice. Our results also demonstrated thatK. pneumoniae bolAincreases the production of siderophore and virulence in Galleria mellonella larvae. Collectively, our results demonstrated thatK. pneumoniaeBolA is a new virulence factor which contributes to survival in different stresses and overcome host defense. These findings are helpful for the research of new treatment strategies forK. pneumoniaeinfection.IMPORTANCEKlebsiella pneumoniaeis an important conditional pathogen causing nosocomial infections and community-acquired infections. It can resistant to multiple antibiotics, causing refractory infections and public health threat. Therefore, new treatments are required to fight the pathogen, and a better understanding of its virulence factors are needed to develop new drugs. Here, we unraveled the role of BolA in survival under different stresses and overcome host defense. Our results suggested thatbolAactively contributes to cell morphology, stresses challenge, cell adhesion and siderophore production that are tightly related to bacterial virulence. Therefore,bolAmutant strain reduces the virulence ofK. pneumoniaein G. mellonella larvae and its colonization ability in mice. These results reportedbolAis a key virulence factor inK. pneumoniae, and they are helpful for research of new therapies to treat this increasingly problematic pathogen.
Extensive OMICS resource for Sf21 and Tni cell lines
Authors: Galik Bence , Landry Jonathan J.M. , Kirkpatrick Joanna M. , Fritz Markus Hsi-Yang , Baying Bianka , Blake Jonathon , Haase Bettina , Collier Paul G. , Hercog Rajna , Pavlinic Dinko , Stolt-Bergner Peggy , Besir Hüseyin , Remans Kim , Gyenesei Attila , Benes Vladimir 
Published: April 2021

AbstractInsect-derived cell lines, fromSpodoptera frugiperda(Sf21) and fromTrichoplusia ni(Tni), are the two most widely used cell lines for recombinant protein expression in combination with the Baculoviral Expression Vector System (BEVS). Genomic sequences and annotations are still incomplete for Sf21 and absent for Tni. In this study, we present an approach using different sequencing data types, including short-read sequencing, long synthetic and Oxford Nanopore reads, to build genomes. The Sf21 and Tni assemblies contain 4,020 scaffolds of 463 Mb in size with N50 of 364 Kb and 2,954 scaffolds of 332 Mb in size with N50 of 326 Kb, respectively. Furthermore, we built a new gene prediction workflow, which integrates transcriptome and proteome information using pre-existing tools. Using this approach, we predicted 21,506 Sf21 and 14,159 Tni genes, generated and integrated proteomic datasets to validate predicted genes and could identify 5577 and 4919 proteins in the Sf21 and Tni cell lines respectively. This integrative approach could be theoretically applied to any uncharacterized genome and result in valuable new resources. With this information available, Sf21 and Tni cells will become even better tools for protein expression and could be used in a wider range of applications, from promoter identification to genome engineering and editing.
Emergent Spatiotemporal Population Dynamics with Cell-Length Control of Synthetic Microbial Consortia
Authors: Winkle James J. , Karamched Bhargav R. , Bennett Matthew R. , Ott William , Josić Krešimir 
Published: April 2021

AbstractIncreased complexity of engineered microbial biocircuits highlights the need for distributed cell functionality due to concomitant increases of metabolic and regulatory burdens imposed on single-strain topologies. Distributed systems, however, introduce additional challenges since consortium composition and spatiotemporal dynamics of constituent strains must be robustly controlled to achieve desired circuit behaviors. Here, we address these challenges with a modeling-based investigation of emergent spatiotemporal population dynamics that result from cell-length control of monolayer, two-strain bacterial consortia. We demonstrate that with dynamic control of a strain’s division length, nematic cell alignment in close-packed monolayers can be destabilized. We found this destabilization conferred an emergent, competitive advantage on smaller-length strains—but by mechanisms that differed depending on the spatial patterns of the population. We used complementary modeling approaches to elucidate underlying mechanisms: an agent-based model to simulate detailed mechanical and signaling interactions between the competing strains and a reductive, stochastic lattice model to represent cell-cell interactions with a single rotational parameter. Our modeling suggests that spatial strain-fraction oscillations can be generated when cell-length control is coupled to quorum-sensing signaling in negative feedback topologies. Our research employs novel methods of population control and points the way to programming strain fraction dynamics in consortial synthetic biology.Engineered microbial collectives are more versatile and robust than single strain populations. However, the function of such collectives is sensitive to their spatiotemporal organization. Here, we demonstrate control of the spatiotemporal composition of synthetic microbial consortia by dynamically modulating the average cell length of constituent strains. Such modulation confers an emergent “mechanical fitness” advantage upon the shorter length strain. We used both a biophysically realistic agent-based model to test the impact of cell shape on spatiotemporal dynamics and a conceptually simpler stochastic lattice model to explain the essential mechanisms driving the dynamics.
Numerical investigations on the hydrogen jet pressure variations in a strut based scramjet combustor
Authors: Suppandipillai Jeyakumar , Kandasamy Jayaraman , Sivakumar R. , Karaca Mehmet , K. Karthik 
Published: April 2021

Purpose
This paper aims to study the influences of hydrogen jet pressure on flow features of a strut-based injector in a scramjet combustor under-reacting cases are numerically investigated in this study.

Design/methodology/approach
The numerical analysis is carried out using Reynolds Averaged Navier Stokes (RANS) equations with the Shear Stress Transport k-ω turbulence model in contention to comprehend the flow physics during scramjet combustion. The three major parameters such as the shock wave pattern, wall pressures and static temperature across the combustor are validated with the reported experiments. The results comply with the range, indicating the adopted simulation method can be extended for other investigations as well. The supersonic flow characteristics are determined based on the flow properties, combustion efficiency and total pressure loss.

Findings
The results revealed that the augmentation of hydrogen jet pressure via variation in flame features increases the static pressure in the vicinity of the strut and destabilize the normal shock wave position. Indeed, the pressure of the mainstream flow drives the shock wave toward the upstream direction. The study perceived that once the hydrogen jet pressure is reached 4 bar, the incoming flow attains a subsonic state due to the movement of normal shock wave ahead of the strut. It is noticed that the increase in hydrogen jet pressure in the supersonic flow field improves the jet penetration rate in the lateral direction of the flow and also increases the total pressure loss as compared with the baseline injection pressure condition.

Practical implications
The outcome of this research provides the influence of fuel injection pressure variations in the supersonic combustion phenomenon of hypersonic vehicles.

Originality/value
This paper substantiates the effect of increasing hydrogen jet pressure in the reacting supersonic airstream on the performance of a scramjet combustor.
Do privacy concerns determine online information disclosure? The case of internet addiction
Authors: Thompson Nik , Ahmad Atif , Maynard Sean 
Published: April 2021

PurposeIt is a widely held belief that users make a rational cost-benefit decision when choosing whether to disclose information online. Yet, in the privacy context, the evidence is far from conclusive suggesting that strong and as-yet unmeasured influences on behaviour may exist. This paper aims to demonstrate one such link – the effect of internet addiction on information disclosure.Design/methodology/approachData from 216 Web users was collected regarding their perceptions on privacy and information disclosure intentions as well as avoidance behaviour, an element of internet addiction. Using a research model based on the Privacy Calculus theory, structural equation modelling was applied to quantify the determinants of online disclosure under various conditions.FindingsThe authors show that not all aspects of privacy (a multi-dimensional construct) influence information disclosure. While concerns about data collection influence self-disclosure behaviour, the level of awareness about privacy does not. They next examine the impact of internet addiction on these relationships, finding that internet addiction weakens the influence of privacy concerns to the point of non-significance.Originality/valueThe authors highlight some of the influences of self-disclosure behaviour, showing that some but not all aspects of privacy are influential. They also demonstrate that there are powerful influences on user behaviour that have not been accounted for in prior work; internet addiction is one of these factors. This provides some of the first evidence of the potentially deleterious effect of internet addiction on the privacy calculus.
Impact of online social media activities on marketing of green products
Authors: Gupta Meesha , Syed Asif Ali 
Published: April 2021

Purpose
The purpose of this paper is to identify, empirically analyze and validate the relationship between various social media marketing activities influencing the attitude of Indian consumers toward green products and their willingness to pay (WTP) a premium price for them.

Design/methodology/approach
Data was collected from 536 respondents who not only use various social media channels for purchases and gaining information but also prefer and buy green products across India. Exploratory factor analysis was done for the refinement and validation of the questionnaire items, and structural equation modeling was used to analyze the relationship among various variables.

Findings
The findings of the empirical study confer consumer’s attitude toward green products being shaped by four main social media marketing activities, namely, word of mouth, interaction, entertainment and customization, whereas trendiness was found to be insignificant toward predicting consumer’s attitude. The positive attitude so formed has a significant and positive impact on the willingness of consumers to pay a premium price for green products.

Originality/value
This research takes a lead in analyzing the construct WTP a premium for green products because this has been comparably less explored in Indian context. As spending a little extra by Indian consumers is a super sensitive issue, studying their willingness is therefore an important construct to be taken into account.
Study on tribological behavior, lubrication and anti-corrosion properties of W/O microemulsion for cold rolling of copper strip
Authors: Wu Hao , Xiong Sang , Lin Wei , Kong Fanxin 
Published: April 2021

PurposeThe purpose of this paper is to improve lubrication and anti-corrosion properties of the water-in-oil (W/O) microemulsion for rolling of copper strip and sheet to replace the traditional rolling oil.Design/methodology/approachThe W/O microemulsion is prepared by using hydrogenated base oil, a deionized aqueous solution of 0.03 mol/L of Na2SO4 and composite emulsifier such as Sp20, Tx-7 or sodium petroleum sulfonate. Tribological behavior of the microemulsions and traditional cold rolling oil was conducted by MR-10A four-ball tester. The lubrication performance of microemulsion for cold rolling of copper strip was performed by cold-rolling experiment. The morphology of worn surface and the rolled copper was characterized. Anti-corrosion properties of microemulsion for rolled copper was investigated, and the corroded surface was analyzed by X-ray photoelectron spectrometer (XPS).FindingsThe results show that the extreme pressure and antiwear properties of the microemulsions have been improved; the average friction coefficient of the improved microemulsion is 0.065, which is 30% lower than the commercial cold rolling oil. For cold rolling of copper strip, the microemulsion has a higher thinning effect than the commercial cold rolling oil, and a smooth surface is obtained and the surface roughness (Sa) is decreased by 6.8%. The XPS analysis indicated microemulsion adsorbed on the copper surface mitigate the corrosion of oils.Originality/valueThis paper used the prepared W/O microemulsion as a new lubricant in the process of rolling for copper strip and sheet in industry, demonstrating the microemulsion has broad application prospects in the future.Peer reviewThe peer review history for this article is available at:https://publons.com/publon/10.1108/ILT-06-2020-0227/
Novel design method for multi-configuration dexterous hand based on a gesture primitives analysis
Authors: Tao Zhicheng , Sheng Shineng , Chen Zhipei , Bao Guanjun 
Published: April 2021

Purpose
This paper aims to propose a novel method based on a gesture primitives analysis of human daily grasping tasks for designing dexterous hands with various grasping and in-hand manipulation abilities, which simplifies the complex and redundant humanoid five-finger hand system.

Design/methodology/approach
First, the authors developed the fingers and the joint configuration with a series of gesture primitives configurations and the modular virtual finger scheme, refined from the daily work gesture library by principal component analysis. Then, the authors optimized the joint degree-of-freedom configuration with the bionic design analysis of the anatomy, and the authors optimized the dexterity workspace. Furthermore, the adaptive fingertip and routing structure were designed based on the dexterous manipulation theory. Finally, the effectiveness of the design method was experimentally validated.

Findings
A novel lightweight three-finger and nine-degree-of-freedom dexterous hand with force/position perception was designed. The proposed routing structure was shown to have the capability of mapping the relationship between the joint space and actuator space. The adaptive fingertip with an embedded force sensor can effectively increase the robustness of the grasping operation. Moreover, the dexterous hand can grasp various objects in different configurations and perform in-hand manipulation dexterously.

Originality/value
The dexterous hand design developed in this study is less complex and performs better in dexterous manipulation than previous designs.
Self-reported face recognition abilities for own and other-race faces
Authors: Estudillo Alejandro J. 
Published: April 2021

Purpose
The other-race effect shows that people are better recognizing faces from their own-race compared to other-race faces. This effect can have dramatic consequences in applied scenarios whereby face identification is paramount, such as eyewitness identification. This paper aims to investigate whether observers have insights into their ability to recognize other-race faces.

Design/methodology/approach
Chinese ethnic observers performed objective measures of own- and other-race face recognition – the Cambridge Face Memory Test Chinese and the Cambridge Face Memory Test original; the PI20 – a 20-items self-reported measured of general face recognition abilities; and the ORE20 – a new developed 20-items self-reported measure of other-race face recognition.

Findings
Recognition of own-race faces was better compared to other-race faces. This effect was also evident at a phenomenological level, as observers reported to be worse recognizing other-race faces compared to own-race faces. Additionally, although a moderate correlation was found between own-race face recognition abilities and the PI20, individual differences in the recognition of other-race faces was only poorly associated with observers’ scores in the ORE20.

Research limitations/implications
These results suggest that observers’ insights to recognize faces are more consistent and reliable for own-race faces.

Practical implications
Self-reported measures of other-race recognition could produce misleading results. Thus, when evaluating eyewitness’ accuracy identifying other-race faces, objective measures should be used.

Originality/value
In contrast to own race recognition, people have very limited insights into their recognition abilities for other race faces.
Measurement of intangible assets using higher-order construct model
Authors: Tefera Chalachew Almaw , Hunsaker William D. 
Published: April 2021

PurposeIntangible assets (IA) have been described as a multidimensional concept. However, there is a gap in research that considers IAs as a higher-order construct (HOC). Therefore, this paper aims to focus on demonstrating how IAs can be properly specified and measured as HOCs, given IA’s four major dimensions in small- and medium-sized enterprises (SMEs).Design/methodology/approachA HOC model is applied using a partial least squares–structural equation modeling (PLS-SEM) repeated-indicator approach. This approach helps validate the argument for considering IAs as HOCs by testing the basic requirements of the construct model.FindingsThe main finding from testing the model’s reliability, validity, multicollinearity and redundancy analysis indicates that the establishment of IAs as a HOC model is valid. Additionally, the simulation study results support the use of the proposed model.Research limitations/implicationsThe proposed model is believed to enhance the proper understanding of IAs and their relationship with organizational outcomes. Additionally, the model can inform organizational management of the integrative approach needed to effectively synchronize their strategic resources for improved performance of entrepreneurially growing SMEs.Originality/valueThis paper is the first to show the measurement of IAs as HOCs using PLS-SEM, based on IA’s updated dimensions. The authors believe that this paper paves the way for future discussion on IA measurement to conduct rigorous impactful studies with larger sample size.