bluuebunny/crossref_metadata_2025_split huggingface dataset:
Reduced ectoparasite load, body mass and blood haemolysis in Eurasian kestrels (Falco tinnunculus) along an urban–rural gradient
Authors: Wemer Laura , Hegemann Arne , Isaksson Caroline , Nebel Carina , Kleindorfer Sonia , Gamauf Anita , Adrion Marius , Sumasgutner Petra 
Published: September 2021

AbstractUrbanisation is proceeding at an alarming rate which forces wildlife to either retreat from urban areas or cope with novel stressors linked to human presence and activities. For example, urban stressors like anthropogenic noise, artificial light at night and chemical pollution can have severe impacts on the physiology of wildlife (and humans), in particular the immune system and antioxidant defences. These physiological systems are important to combat and reduce the severity of parasitic infections, which are common among wild animals. One question that then arises is whether urban-dwelling animals, whose immune and antioxidant system are already challenged by the urban stressors, are more susceptible to parasitic infections. To assess this, we studied nestlings of Eurasian kestrels (Falco tinnunculus) in Vienna, Austria, during 2015 and 2017. We measured biomarkers of innate immune function, oxidative stress and body mass index and ectoparasite infection intensity in 143 nestlings (from 56 nests) along an urban gradient. Nestlings in more urbanised areas had overall fewer ectoparasites, lower haemolysis (complement activity) and lower body mass index compared to nestlings in less urbanised areas. None of the other immune or oxidative stress markers were associated with the urban gradient. Despite some non-significant results, our data still suggest that kestrel nestlings experience some level of reduced physiological health, perhaps as a consequence of exposure to more urban stressors or altered prey availability in inner-city districts even though they had an overall lower ectoparasite burden in these heavily urbanised areas.
AI under great uncertainty: implications and decision strategies for public policy
Authors: Nordström Maria 
Published: September 2021

AbstractDecisions where there is not enough information for a well-informed decision due to unidentified consequences, options, or undetermined demarcation of the decision problem are called decisions under great uncertainty. This paper argues that public policy decisions on how and if to implement decision-making processes based on machine learning and AI for public use are such decisions. Decisions on public policy on AI are uncertain due to three features specific to the current landscape of AI, namely (i) the vagueness of the definition of AI, (ii) uncertain outcomes of AI implementations and (iii) pacing problems. Given that many potential applications of AI in the public sector concern functions central to the public sphere, decisions on the implementation of such applications are particularly sensitive. Therefore, it is suggested that public policy-makers and decision-makers in the public sector can adopt strategies from the argumentative approach in decision theory to mitigate the established great uncertainty. In particular, the notions of framing and temporal strategies are considered.
Severe bicompartmental bone bruise is associated with rotatory instability in anterior cruciate ligament injury
Authors: Agostinone Piero , Di Paolo Stefano , Lucidi Gian Andrea , Dal Fabbro Giacomo , Grassi Alberto , Zaffagnini Stefano 
Published: September 2021

Abstract
Purpose
The presence and severity of bone bruise is more and more investigated in the non-contact anterior cruciate ligament (ACL) injury context. Recent studies have advocated a correlation between bone bruise and preoperative knee laxity. The aim of the present study was to investigate the correlation between bone bruise and preoperative rotatory knee laxity.

Methods
Twenty-nine patients (29.1 ± 9.8 years) with MRI images at a maximum of 3 months after ACL injury (1.6 ± 0.8 months) were included. The bone bruise severity was evaluated according to the International Cartilage Repair Society (ICRS) scale for lateral femoral condyle, lateral tibial plateau, medial femoral condyle, and medial tibial plateau. The intraoperative rotational knee laxity was evaluated through a surgical navigation system in terms of internal–external rotation at 30° and 90° of knee flexion (IE30, IE90) and internal–external rotation and acceleration during pivot-shift test (PS IE, PS ACC). The KOOS score was also collected. The association between ICRS grade of bone bruise and rotational laxity or KOOS was investigated.

Results
Significant correlation (p < 0.05) was found between the bone bruise severity on the medial tibial plateau and rotational laxity (IE90, PS IE, and PS ACC) and between the severity of bone bruise on femoral lateral condyle and KOOS-Symptoms sub-score. The presence of bone bruise on the medial tibial plateau was significantly associated with a lateral femoral notch sign > 2 mm (very strong odds ratio). No kinematical differences were found between none-to-deep and extensive-generalized lateral bone bruise, while higher IE30 and IE90 were found in extensive-generalized bicompartmental bone bruise than isolated extensive-generalized lateral bone bruise.

Conclusion
A severe bicompartmental bone bruise was related to higher rotatory instability in the intraoperative evaluation of ACL deficient knees. The severity of edema on the medial tibial plateau was directly correlated with higher intraoperative pivot shift, and the size of edema on the lateral femoral condyle was associated with lower preoperative clinical scores.

Level of evidence
Level II.
Modeling ocean-induced rapid Earth rotation variations: an update
Authors: Harker Alexander A. , Schindelegger Michael , Ponte Rui M. , Salstein David A. 
Published: September 2021

AbstractWe revisit the problem of modeling the ocean’s contribution to rapid, non-tidal Earth rotation variations at periods of 2–120 days. Estimates of oceanic angular momentum (OAM, 2007–2011) are drawn from a suite of established circulation models and new numerical simulations, whose finest configuration is on a "Image missing"$$^\circ $$

∘

grid. We show that the OAM product by the Earth System Modeling Group at GeoForschungsZentrum Potsdam has spurious short period variance in its equatorial motion terms, rendering the series a poor choice for describing oceanic signals in polar motion on time scales of less than $$\sim $$
∼
2 weeks. Accounting for OAM in rotation budgets from other models typically reduces the variance of atmosphere-corrected geodetic excitation by $$\sim $$
∼
54% for deconvolved polar motion and by $$\sim $$
∼
60% for length-of-day. Use of OAM from the "Image missing"$$^\circ $$

∘

model does provide for an additional reduction in residual variance such that the combined oceanic–atmospheric effect explains as much as 84% of the polar motion excitation at periods < 120 days. Employing statistical analysis and bottom pressure changes from daily Gravity Recovery and Climate Experiment solutions, we highlight the tendency of ocean models run at a 1$$^\circ $$

∘

grid spacing to misrepresent topographically constrained dynamics in some deep basins of the Southern Ocean, which has adverse effects on OAM estimates taken along the 90$$^\circ $$

∘

meridian. Higher model resolution thus emerges as a sensible target for improving the oceanic component in broader efforts of Earth system modeling for geodetic purposes.
The effect of potent CYP2D6 inhibition on the pharmacokinetics and safety of deutetrabenazine in healthy volunteers
Authors: Schneider F. , Stamler D. , Bradbury M. J. , Loupe P. S. , Gordon M. F. , Rabinovich-Guilatt L. 
Published: September 2021

Abstract
Purpose
Deutetrabenazine is a deuterated form of tetrabenazine with a confirmed lower rate of CYP2D6 metabolism of the active metabolites, α- and β-HTBZ. In this study, we assessed the effect of paroxetine, a potent CYP2D6 inhibitor, on the pharmacokinetics and safety of deutetrabenazine and its metabolites.

Methods
In this open-label sequential drug-drug-interaction study, 24 healthy adults who were CYP2D6 extensive or intermediate metabolizers received a single deutetrabenazine 22.5-mg oral dose on days 1 and 11 and a single paroxetine 20-mg oral daily dose on days 4–12. Pharmacokinetics of deutetrabenazine and its metabolites were assessed on days 1–4 and 11–14. Paroxetine trough concentrations were obtained pre-dose on days 9–13. Safety examinations occurred throughout the study.

Results
Paroxetine administered under steady-state conditions, increased exposure of the deuterated active metabolites, α-HTBZ (1.2-fold Cmax and 1.8-fold AUC0–∞) and β-HTBZ (2.1-fold Cmax and 5.6-fold AUC0–∞), and correspondingly, 1.6-fold Cmax and threefold AUC0–∞ for total (α + β)-HTBZ. Sixteen subjects reported 45 adverse events and most were mild. Headache was the most common AE reported 8 times by 7 subjects (5 following paroxetine alone; 2 following deutetrabenazine + paroxetine).

Conclusions
Paroxetine-induced increases in exposure to the active deutetrabenazine metabolites were less than those previously reported for tetrabenazine, a finding expected to reduce the burden of drug interaction. In addition, single doses of 22.5 mg deutetrabenazine, when given alone or in the presence of steady-state paroxetine (20 mg daily), were safe.
Increased biosynthesis of acetyl-CoA in the yeast Saccharomyces cerevisiae by overexpression of a deregulated pantothenate kinase gene and engineering of the coenzyme A biosynthetic pathway
Authors: Olzhausen Judith , Grigat Mathias , Seifert Larissa , Ulbricht Tom , Schüller Hans-Joachim 
Published: September 2021

AbstractCoenzyme A (CoA) and its derivatives such as acetyl-CoA are essential metabolites for several biosynthetic reactions. In the yeast S. cerevisiae, five enzymes (encoded by essential genes CAB1-CAB5; coenzyme A biosynthesis) are required to perform CoA biosynthesis from pantothenate, cysteine, and ATP. Similar to enzymes from other eukaryotes, yeast pantothenate kinase (PanK, encoded by CAB1) turned out to be inhibited by acetyl-CoA. By genetic selection of intragenic suppressors of a temperature-sensitive cab1 mutant combined with rationale mutagenesis of the presumed acetyl-CoA binding site within PanK, we were able to identify the variant CAB1 W331R, encoding a hyperactive PanK completely insensitive to inhibition by acetyl-CoA. Using a versatile gene integration cassette containing the TPI1 promoter, we constructed strains overexpressing CAB1 W331R in combination with additional genes of CoA biosynthesis (CAB2, CAB3, HAL3, CAB4, and CAB5). In these strains, the level of CoA nucleotides was 15-fold increased, compared to a reference strain without additional CAB genes. Overexpression of wild-type CAB1 instead of CAB1 W331R turned out as substantially less effective (fourfold increase of CoA nucleotides). Supplementation of overproducing strains with additional pantothenate could further elevate the level of CoA (2.3-fold). Minor increases were observed after overexpression of FEN2 (encoding a pantothenate permease) and deletion of PCD1 (CoA-specific phosphatase). We conclude that the strategy described in this work may improve the efficiency of biotechnological applications depending on acetyl-CoA.Key points• A gene encoding a hyperactive yeast pantothenate kinase (PanK) was constructed.• Overexpression of CoA biosynthetic genes elevated CoA nucleotides 15-fold.• Supplementation with pantothenate further increased the level of CoA nucleotides.
Prediction of TERTp-mutation status in IDH-wildtype high-grade gliomas using pre-treatment dynamic [18F]FET PET radiomics
Authors: Li Zhicong , Kaiser Lena , Holzgreve Adrien , Ruf Viktoria C. , Suchorska Bogdana , Wenter Vera , Quach Stefanie , Herms Jochen , Bartenstein Peter , Tonn Jörg-Christian , Unterrainer Marcus , Albert Nathalie L. 
Published: September 2021

Abstract
Purpose
To evaluate radiomic features extracted from standard static images (20–40 min p.i.), early summation images (5–15 min p.i.), and dynamic [18F]FET PET images for the prediction of TERTp-mutation status in patients with IDH-wildtype high-grade glioma.

Methods
A total of 159 patients (median age 60.2 years, range 19–82 years) with newly diagnosed IDH-wildtype diffuse astrocytic glioma (WHO grade III or IV) and dynamic [18F]FET PET prior to surgical intervention were enrolled and divided into a training (n = 112) and a testing cohort (n = 47) randomly. First-order, shape, and texture radiomic features were extracted from standard static (20–40 min summation images; TBR20–40), early static (5–15 min summation images; TBR5–15), and dynamic (time-to-peak; TTP) images, respectively. Recursive feature elimination was used for feature selection by 10-fold cross-validation in the training cohort after normalization, and logistic regression models were generated using the radiomic features extracted from each image to differentiate TERTp-mutation status. The areas under the ROC curve (AUC), accuracy, sensitivity, specificity, and positive and negative predictive value were calculated to illustrate diagnostic power in both the training and testing cohort.

Results
The TTP model comprised nine selected features and achieved highest predictability of TERTp-mutation with an AUC of 0.82 (95% confidence interval 0.71–0.92) and sensitivity of 92.1% in the independent testing cohort. Weak predictive capability was obtained in the TBR5–15 model, with an AUC of 0.61 (95% CI 0.42–0.80) in the testing cohort, while no predictive power was observed in the TBR20–40 model.

Conclusions
Radiomics based on TTP images extracted from dynamic [18F]FET PET can predict the TERTp-mutation status of IDH-wildtype diffuse astrocytic high-grade gliomas with high accuracy preoperatively.
Benchmarking prediction skill in binary El Niño forecasts
Authors: Hu Xinjia , Eichner Jan , Faust Eberhard , Kantz Holger 
Published: September 2021

AbstractReliable El Niño Southern Oscillation (ENSO) prediction at seasonal-to-interannual lead times would be critical for different stakeholders to conduct suitable management. In recent years, new methods combining climate network analysis with El Niño prediction claim that they can predict El Niño up to 1 year in advance by overcoming the spring barrier problem (SPB). Usually this kind of method develops an index representing the relationship between different nodes in El Niño related basins, and the index crossing a certain threshold is taken as the warning of an El Niño event in the next few months. How well the prediction performs should be measured in order to estimate any improvements. However, the amount of El Niño recordings in the available data is limited, therefore it is difficult to validate whether these methods are truly predictive or their success is merely a result of chance. We propose a benchmarking method by surrogate data for a quantitative forecast validation for small data sets. We apply this method to a naïve prediction of El Niño events based on the Oscillation Niño Index (ONI) time series, where we build a data-based prediction scheme using the index series itself as input. In order to assess the network-based El Niño prediction method, we reproduce two different climate network-based forecasts and apply our method to compare the prediction skill of all these. Our benchmark shows that using the ONI itself as input to the forecast does not work for moderate lead times, while at least one of the two climate network-based methods has predictive skill well above chance at lead times of about one year.
Magnesium intake and vascular structure and function: the Hoorn Study
Authors: Vermeulen Emma A. , de Jong Hanne B. T. , Blomjous Alexander G. A. , Eelderink Coby , Hoekstra Tiny , Elders Petra J. M. , de Borst Martin H. , Vervloet Marc G. , van Ballegooijen Adriana J. , Beulens Joline W. 
Published: September 2021

Abstract
Purpose
Circulating and dietary magnesium have been shown to be inversely associated with the prevalence of cardiovascular disease (CVD) and mortality in both high and low-risk populations. We aimed to examine the association between dietary magnesium intake and several measures of vascular structure and function in a prospective cohort.

Methods
We included 789 participants who participated in the vascular screening sub-cohort of the Hoorn Study, a population-based, prospective cohort study. Baseline dietary magnesium intake was estimated with a validated food frequency questionnaire and categorised in energy-adjusted magnesium intake tertiles. Several measurements of vascular structure and function were performed at baseline and most measurements were repeated after 8 years of follow-up (n = 432). Multivariable linear and logistic regression was performed to study the cross-sectional and longitudinal associations of magnesium intake and intima-media thickness (IMT), augmentation index (Aix), pulse wave velocity (PWV), flow-mediated dilatation (FMD), and peripheral arterial disease (PAD).

Results
Mean absolute magnesium intake was 328 ± 83 mg/day and prior CVD and DM2 was present in 55 and 41% of the participants, respectively. Multivariable regression analyses did not demonstrate associations between magnesium intake and any of the vascular outcomes. Participants in the highest compared to the lowest magnesium intake tertile demonstrated in fully adjusted cross-sectional analyses a PWV of −0.21 m/s (95% confidence interval −1.95, 1.52), a FMD of −0.03% (−0.89, 0.83) and in longitudinal analyses an IMT of 0.01 mm (−0.03, 0.06), an Aix of 0.70% (−1.69, 3.07) and an odds ratio of 0.84 (0.23, 3.11) for PAD

Conclusion
We did not find associations between dietary magnesium intake and multiple markers of vascular structure and function, in either cross-sectional or longitudinal analyses.
Introducing a novel model for simulating large loop excision of the transformation zone (LLETZ) using 3D printing technique
Authors: Kiesel Matthias , Beyers Inga , Kalisz Adam , Wöckel Achim , Herbert Saskia-Laureen , Curtaz Carolin , Diessner Joachim , Joukhadar Ralf , Wulff Christine 
Published: September 2021

Abstract
Purpose
Electrosurgery is the gold-standard procedure for the treatment of cervical dysplasia. The quality of the outcome depends on the accuracy of performance, which underlines the role of adequate training of surgeons, especially, as this procedure is often performed by novice surgeons. According to our knowledge, medical simulation has up until now lacked a model, which focuses on realistically simulating the treatment of cervical dysplasia with the concerning anatomy.

Methods and result
In our work, we present a model created using 3D printing for holistically simulating diagnostic, as well as surgical interventions of the cervix, as realistically as possible.

Conclusion
This novel simulator is compared to an existing model and both are evaluated. By doing so, we aim to provide novice gynecologists with standardized and high-quality simulation models for practicing to improve their proficiency.
Changes in gynecologic and breast cancer diagnoses during the first wave of the COVID-19 pandemic: analysis from a tertiary academic gyneco-oncological center in Germany
Authors: Kaltofen Till , Hagemann Friederike , Harbeck Nadia , Wuerstlein Rachel , Kost Bernd Peter , Burges Alexander , Mahner Sven , Trillsch Fabian 
Published: September 2021

Abstract
Purpose
With the beginning of 2021, the world has been suffering from the COVID-19 pandemic for more than 1 year. More and more, we are able to evaluate side effects of the pandemic in the healthcare sector. A negative impact on cancer diagnoses is one of them. Careful observation of trends in an academic gyneco-oncological context appears important to identify potential negative developments.

Methods
We analyzed the case number of gynecologic and breast cancer diagnoses in the period from January to June 2020 compared to 2019 and during the period of the first general German lockdown (March 22nd until May 5th 2020). Patients were characterized by age, tumor type, FIGO or TNM stage and presence of symptoms at initial hospital presentation.

Results
The frequency of newly diagnosed gynecologic and breast cancer cases from beginning of January until end of June changed by − 10% and by − 12% during the lockdown in 2020 compared to 2019. In both periods, reduction of breast cancer cases was relatively larger than decrease of gynecologic cancers. Moreover, median patient age decreased. For the first half of 2020, we found a shift towards higher tumor stages (N+/M1 or FIGO III–IV). During the lockdown period, the appearance of tumor-associated symptoms at diagnosis increased by about 12%.

Conclusion
This analysis illustrates the anticipated general decrease in diagnoses of primary cancers during the lockdown periods in 2020 due to COVID-19 pandemic for gynecologic and breast cancer cases.
Internal validation and improvement of mitochondrial genome sequencing using the Precision ID mtDNA Whole Genome Panel
Authors: Faccinetto Christian , Sabbatini Daniele , Serventi Patrizia , Rigato Martina , Salvoro Cecilia , Casamassima Gianluca , Margiotta Gianluca , De Fanti Sara , Sarno Stefania , Staiti Nicola , Luiselli Donata , Marino Alberto , Vazza Giovanni 
Published: September 2021

AbstractWith the recent advances in next-generation sequencing (NGS), mitochondrial whole-genome sequencing has begun to be applied to the field of the forensic biology as an alternative to the traditional Sanger-type sequencing (STS). However, experimental workflows, commercial solutions, and output data analysis must be strictly validated before being implemented into the forensic laboratory. In this study, we performed an internal validation for an NGS-based typing of the entire mitochondrial genome using the Precision ID mtDNA Whole Genome Panel (Thermo Fisher Scientific) on the Ion S5 sequencer (Thermo Fisher Scientific). Concordance, repeatability, reproducibility, sensitivity, and heteroplasmy detection analyses were assessed using the 2800 M and 9947A standard control DNA as well as typical casework specimens, and results were compared with conventional Sanger sequencing and another NGS sequencer in a different laboratory. We discuss the strengths and limitations of this approach, highlighting some issues regarding noise thresholds and heteroplasmy detection, and suggesting solutions to mitigate these effects and improve overall data interpretation. Results confirmed that the Precision ID Whole mtDNA Genome Panel is highly reproducible and sensitive, yielding useful full mitochondrial DNA sequences also from challenging DNA specimens, thus providing further support for its use in forensic practice.