bluuebunny/crossref_metadata_2025_split huggingface dataset:
Long term treatment with omalizumab in adolescent with refractory solar urticaria
Authors: Iannelli Mauro , Passanisi Stefano , Crisafulli Giuseppe , Arasi Stefania , Caminiti Lucia , Zirilli Giuseppina , Pajno Giovanni B. 
Published: September 2021

AbstractBackgroundSolar urticaria represents an uncomfortable form of chronic inducible urticaria. First and second-line treatments are ineffective in some patients, leading to an impairment in their quality of life. Omalizumab represents a safe therapeutic option in case of refractory solar urticaria.Case presentationWe update a case of a 21-year-old Caucasian girl affected by solar urticaria from the age of 14. Poor disease control was achieved with standard or high-dose of H1-antihistamines. Several omalizumab courses, including a 1-year-long course, were practiced resulting in clinical remission and significant improvement in patient’s quality of life.ConclusionOur experience confirms the effectiveness and safety of omalizumab for the management of refractory solar urticaria. Future studies are awaited in order to monitor long term effects and chronic doses of this treatment, particularly in patients who need concomitant therapy with antihistamines.
Association of deranged cerebrovascular reactivity with brain injury following cardiac arrest: a post-hoc analysis of the COMACARE trial
Authors: Laurikkala Johanna , Aneman Anders , Peng Alexander , Reinikainen Matti , Pham Paul , Jakkula Pekka , Hästbacka Johanna , Wilkman Erika , Loisa Pekka , Toppila Jussi , Birkelund Thomas , Blennow Kaj , Zetterberg Henrik , Skrifvars Markus B. 
Published: September 2021

AbstractBackgroundImpaired cerebrovascular reactivity (CVR) is one feature of post cardiac arrest encephalopathy. We studied the incidence and features of CVR by near infrared spectroscopy (NIRS) and associations with outcome and biomarkers of brain injury.MethodsA post-hoc analysis of 120 comatose OHCA patients continuously monitored with NIRS and randomised to low- or high-normal oxygen, carbon dioxide and mean arterial blood pressure (MAP) targets for 48 h. The tissue oximetry index (TOx) generated by the moving correlation coefficient between cerebral tissue oxygenation measured by NIRS and MAP was used as a dynamic index of CVR with TOx > 0 indicating impaired reactivity and TOx > 0.3 used to delineate the lower and upper MAP bounds for disrupted CVR. TOxwas analysed in the 0–12, 12–24, 24–48 h time-periods and integrated over 0–48 h. The primary outcome was the association between TOxand six-month functional outcome dichotomised by the cerebral performance category (CPC1-2 good vs. 3–5 poor). Secondary outcomes included associations with MAP bounds for CVR and biomarkers of brain injury.ResultsIn 108 patients with sufficient data to calculate TOx, 76 patients (70%) had impaired CVR and among these, chronic hypertension was more common (58% vs. 31%,p = 0.002). Integrated TOxfor 0–48 h was higher in patients with poor outcome than in patients with good outcome (0.89 95% CI [− 1.17 to 2.94] vs. − 2.71 95% CI [− 4.16 to − 1.26],p = 0.05). Patients with poor outcomes had a decreased upper MAP bound of CVR over time (p = 0.001), including the high-normal oxygen (p = 0.002), carbon dioxide (p = 0.012) and MAP (p = 0.001) groups. The MAP range of maintained CVR was narrower in all time intervals and intervention groups (p < 0.05). NfL concentrations were higher in patients with impaired CVR compared to those with intact CVR (43 IQR [15–650] vs 20 IQR [13–199] pg/ml,p = 0.042).ConclusionImpaired CVR over 48 h was more common in patients with chronic hypertension and associated with poor outcome. Decreased upper MAP bound and a narrower MAP range for maintained CVR were associated with poor outcome and more severe brain injury assessed with NfL.Trial registrationClinicalTrials.gov,NCT02698917.
Anabolic–androgenic steroids and dietary supplements among resistance trained individuals in western cities of Saudi Arabia
Authors: Almohammadi Ameen Mosleh , Edriss Anas Mohammed , Enani Turki Talal 
Published: September 2021

Abstract
Background
Anabolic–androgenic steroids (AAS) contain testosterone-like androgens and are used as supplements to improve performance, therapeutic measures, appearance, and muscular development.

Purpose
This study aimed to estimate using anabolic–androgenic steroids (AAS) and good and bad practices about dietary supplements among resistance-trained individuals. It further seeked to determine the use of common drugs and supplements containing anabolic steroids among resistance-trained individuals (who work out at the sports centre) and assess users' knowledge about its side effects.

Methods
A cross-sectional survey was conducted at the sports centres of the western cities of Saudi Arabia. A self-administered questionnaire was used to collect data from 120 male resistance-trained individuals.

Results
The majority of the participants (80%) reported that they had not used any hormonal bodybuilding supplement last year, while 20% said they had used such hormonal supplements. Approximately half (52.5%) of participants reported that they always used dietary supplements. A total of 44.2% of participants possessed inadequate knowledge of these products. The main reason behind the use of hormones and supplements was to increase muscle mass.

Conclusions
A minority of resistance-trained individuals in the studied population frequently misused AAS. However, the results cannot be generalised to the whole of Saudi Arabia. AAS consumption can be reduced by enhancing the level of awareness and knowledge of potential adverse health outcomes.
Novel culture media enhances mononuclear cells from patients with chronic limb-threatening ischemia to increase vasculogenesis and anti-inflammatory effect
Authors: Chruewkamlow Nuttapol , Pruekprasert Kanin , Phutthakunphithak Phakawan , Acharayothin Onchira , Prapassaro Tossapol , Hongku Kiattisak , Hahtapornsawan Suteekhanit , Puangpunngam Nattawut , Chinsakchai Khamin , Wongwanit Chumpol , Ruangsetakit Chanean , Sermsathanasawadi Nuttawut 
Published: September 2021

Abstract
Background
Quality and Quantity culture media (QQ culture media) was reported to enhance vasculogenesis and angiogenesis function of mononuclear cells (MNCs) from healthy volunteers. In this study, MNCs from chronic limb-threatening ischemia (CLTI) patients were cultured in QQ culture media, and then investigated for angiogenesis-related phenotype and function.

Methods
Patients aged ≥ 18 years with CLTI caused by atherosclerosis of the lower extremities were prospectively recruited at Siriraj Hospital (Bangkok, Thailand) during July 2017–December 2018. Peripheral blood mononuclear cells (PBMNCs) were isolated from peripheral blood. PBMNCs were cultured in either QQ culture media or standard culture media. The number of CD34+CD133+ cells, CD206+ cells, CD4+CD25+CD127+ cells, colony formation assay, and human umbilical vein endothelial cell (HUVEC) tube formation assay in MNCs were compared between those cultured in QQ culture media and those cultured in standard culture media.

Results
Thirty-nine patients were included with a mean age of 69 ± 11 years. Diabetes mellitus was found in 25 (64%) patients. The percentage of CD34+CD133+ progenitor cells in MNCs cultured in QQ culture media and in MNCs cultured in standard culture media was 4.91 ± 5.30% and 0.40 ± 0.46%, respectively (p < 0.0001). The percentage of CD206+ cells in MNCs cultured in QQ culture media and in MNCs cultured in standard culture media was 19.31 ± 11.42% and 4.40 ± 2.54%, respectively (p < 0.0001). The percentage of inactive population of T regulatory cells (CD4+CD25+CD127+ cells) in MNCs cultured in standard culture media and in MNCs cultured in QQ culture media was 14.5 ± 10.68% and 1.84 ± 1.37%, respectively (p < 0.0001). The total number of colony-forming units from MNCs cultured in QQ culture media and in MNCs cultured in standard culture media was 8.86 ± 8.35 of 2 × 105 cells/dish, and 0.58 ± 1.05 of 2 × 105 cells/dish, respectively (p < 0.0001). The mean intensity of Dil-Ac-LDL uptake that incorporated into the HUVEC forming tube was 1.37 ± 0.88 in MNCs cultured in QQ culture media, and 0.78 ± 0.41 in MNCs cultured in standard culture media. (p < 0.0003).

Conclusions
MNCs from CLTI patients that were cultured in QQ culture media had a significantly higher number of CD34+CD133+ cells and anti-inflammatory cells, and higher angiogenesis-related function compared to MNCs cultured in standard culture media.
Applications, challenges and prospects of mesenchymal stem cell exosomes in regenerative medicine
Authors: Rezabakhsh Aysa , Sokullu Emel , Rahbarghazi Reza 
Published: September 2021

AbstractRecent advances in the identification and application of different stem cell types have offered alternative therapeutic approaches for clinicians. The lack of successful engraftment, migration into the injured site, loss of functionality and viability, ethical issues, shortage of donated allogeneic stem cells and the possibility of transmission of infectious are the main challenges associated with direct cell transplantation. The discovery and research on exosomes have led to the rise of hopes for the alleviation of different pathologies in regenerative medicine. Exo are nano-sized extracellular vesicles (40–150 nm) and released by each type. These nanoparticles participate in cell-to-cell communication in a paracrine manner. It is thought that the application of Exo can circumvent several drawbacks related to whole-cell therapies. Because of their appropriate size and stability, Exo are touted as therapeutic bullets transferring signaling factors into the acceptor cells in a paracrine manner. Despite these advantages, technologies associated with Exo isolation and purification are challenging because of heterogeneity in exosomal size and cargo. The lack of standard GMP-grade protocols is the main hurdle that limits the extensive application of Exo in the clinical setting. Here, the authors aimed to inspire a logical and realistic vision about problems associated with Exo application in regenerative medicine.
FP-ADMET: a compendium of fingerprint-based ADMET prediction models
Authors: Venkatraman Vishwesh 
Published: September 2021

Abstract
Motivation
The absorption, distribution, metabolism, excretion, and toxicity (ADMET) of drugs plays a key role in determining which among the potential candidates are to be prioritized. In silico approaches based on machine learning methods are becoming increasing popular, but are nonetheless limited by the availability of data. With a view to making both data and models available to the scientific community, we have developed FPADMET which is a repository of molecular fingerprint-based predictive models for ADMET properties.

Summary
In this article, we have examined the efficacy of fingerprint-based machine learning models for a large number of ADMET-related properties. The predictive ability of a set of 20 different binary fingerprints (based on substructure keys, atom pairs, local path environments, as well as custom fingerprints such as all-shortest paths) for over 50 ADMET and ADMET-related endpoints have been evaluated as part of the study. We find that for a majority of the properties, fingerprint-based random forest models yield comparable or better performance compared with traditional 2D/3D molecular descriptors.

Availability
The models are made available as part of open access software that can be downloaded from https://gitlab.com/vishsoft/fpadmet.
On iterative methods for bilevel equilibrium problems
Authors: Jaipranop Chanitnan , Saejung Satit 
Published: September 2021

AbstractWe use the notion of Halpern-type sequence recently introduced by the present authors to conclude two strong convergence theorems for solving the bilevel equilibrium problems proposed by Yuying et al. and some authors. Our result excludes some assumptions as were the cases in their results.
Coupling reduced-order blood flow and cardiac models through energy-consistent strategies: modeling and discretization
Authors: Manganotti Jessica , Caforio Federica , Kimmig François , Moireau Philippe , Imperiale Sebastien 
Published: September 2021

AbstractIn this work we provide a novel energy-consistent formulation for the classical 1D formulation of blood flow in an arterial segment. The resulting reformulation is shown to be suitable for the coupling with a lumped (0D) model of the heart that incorporates a reduced formulation of the actin-myosin interaction. The coupling being consistent with energy balances, we provide a complete heart-circulation model compatible with thermodynamics hence stable numerically and informative physiologically. These latter two properties are verified by numerical experiments.
A cross-sectional examination of the simultaneous association of four emotion regulation strategies with abnormal eating behaviours among women in Japan
Authors: Murayama Yasuo , Ohya Aiko 
Published: September 2021

Abstract
Background
Research has suggested an association between emotion regulation strategies (ERSs) and abnormal eating behaviours/attitudes (AEB), and many studies have examined the association of one particular ERS with AEB. Additionally, different ERSs are reported to be strongly correlated with each other. Therefore, the associations between an individual ERS and AEB, reported previously, may be spurious. The present cross-sectional study aims to examine the simultaneous associations of four ERSs (brooding, reflection, expressive suppression, cognitive reappraisal) with AEB in a sample of women in Japan.

Methods
The participants comprised 1528 Japanese women (Mage = 40.65 years, SDage = 10.22 years, range 21–59). They self-reported the frequency at which they use these ERSs, their levels of AEB (i.e. drive for thinness, bulimic symptoms), and the confounding variables (e.g. psychological distress and BMI) online. AEB was measured using the Japanese version of the 91-item Eating Disorder Inventory; brooding and reflection were measured using the Japanese version of the Rumination Response Scale; individual differences in the use of reappraisal and expression suppression was measured using the Japanese version of the Emotion Regulation Questionnaire (J-ERQ); and participants’ psychological distress was assessed using the Kessler 6 Japanese version (K6-J).

Results
Correlation analyses revealed that all ERSs were positively correlated with AEB. However, regression analyses revealed inconsistent findings. In the regression model, after controlling for the confounding variables, only brooding indicated a positive association with the drive for thinness. Regarding bulimic symptoms, all ERSs showed a positive association, except reappraisal, which had a weak, negative association.

Conclusion
These results suggest that brooding is related to the symptom levels of both eating disorders among women, whereas, the other ERSs are related to those of bulimic symptoms only. However, further research is required to clarify the causal relations between AEB and ERSs.
High-throughput and high-sensitivity capillary electrophoresis–mass spectrometry method for sulfur-containing amino acids
Authors: Zhao Jieyu , Dong Bing , Zhao Yanni , Yang Jun , Pei Pei , Ji Xianbing , Quan Yulian 
Published: September 2021

AbstractBiological thiol amino acids have been suggested as biomarkers for pathological changes because they are reactive chemicals that participate in various physiological processes. In this study, multisegmented injection capillary electrophoresis–mass spectrometry with online sample preconcentration was used for analysis of thiol amino acids and intermediates of sulfur metabolism in human glioma cell line U-251 with high accuracy, throughput, and sensitivity. This was achieved using multiple, large-volume injections for online sample preconcentration. The 16 intermediates of sulfur metabolism had a good linear correlation coefficient range of 0.984–1 and the limit of detection range was 1.4–203.9 ng/mL. The recovery ranges of most amino acids were 88.1–114.5%, 89.0–104.3%, and 76.9–104.5% at 0.3, 0.75, and 1.5 μg/mL, respectively. The relative standard deviation ranges for the inter- and intra-day precision were 1.8–10.7% and 4.3–18.8%, respectively. Compared with the traditional injection method, the analytical time for compounds in sulfur metabolism was reduced to 4 min/sample, the method throughput was enhanced five times, and the sensitivity was increased 14.4–33.1 times. Customized injection sequences were applied in experimental optimization. The developed method simplified the experimental optimization to one injection and is suitable for the analysis of sulfur metabolites in biological samples and has high sensitivity, throughput, speed, and accuracy.
Convolutional neural network optimizes the application of diffusion kurtosis imaging in Parkinson’s disease
Authors: Sun Junyan , Chen Ruike , Tong Qiqi , Ma Jinghong , Gao Linlin , Fang Jiliang , Zhang Dongling , Chan Piu , He Hongjian , Wu Tao 
Published: September 2021

Abstract
Objectives
The literature regarding the use of diffusion-tensor imaging-derived metrics in the evaluation of Parkinson’s disease (PD) is controversial. This study attempted to assess the feasibility of a deep-learning-based method for detecting alterations in diffusion kurtosis measurements associated with PD.

Methods
A total of 68 patients with PD and 77 healthy controls were scanned using scanner-A (3 T Skyra) (DATASET-1). Meanwhile, an additional five healthy volunteers were scanned with both scanner-A and an additional scanner-B (3 T Prisma) (DATASET-2). Diffusion kurtosis imaging (DKI) of DATASET-2 had an extra b shell compared to DATASET-1. In addition, a 3D-convolutional neural network (CNN) was trained from DATASET-2 to harmonize the quality of scalar measures of scanner-A to a similar level as scanner-B. Whole-brain unpaired t test and Tract-Based Spatial Statistics (TBSS) were performed to validate the differences between the PD and control groups using the model-fitting method and CNN-based method, respectively. We further clarified the correlation between clinical assessments and DKI results.

Results
An increase in mean diffusivity (MD) was found in the left substantia nigra (SN) in the PD group. In the right SN, fractional anisotropy (FA) and mean kurtosis (MK) values were negatively correlated with Hoehn and Yahr (H&Y) scales. In the putamen (Put), FA values were positively correlated with the H&Y scales. It is worth noting that these findings were only observed with the deep learning method. There was neither a group difference nor a correlation with clinical assessments in the SN or striatum exceeding the significance level using the conventional model-fitting method.

Conclusions
The CNN-based method improves the robustness of DKI and can help to explore PD-associated imaging features.
A case of appendiceal ganglioneuroma in neurofibromatosis type 1
Authors: Shimizu Tadaaki , Hondo Nao , Miyagawa Yusuke , Kitazawa Masato , Muranaka Futoshi , Tokumaru Shigeo , Nakamura Satoshi , Koyama Makoto , Yamamoto Yuta , Ehara Takehito , Miyazaki Satoru , Iijima Yasuhiro , Iwaya Mai , Soejima Yuji 
Published: September 2021

Abstract
Background
Neurofibromatosis type 1 is an autosomal dominant inherited disease associated with multiple skin neurofibromas or other neurogenic tumors, such as nodular plexiform neurinoma or cerebrospinal tumor. Gastrointestinal stromal tumors are often complicated in patients with neurofibromatosis type 1, although involvement of the appendix is rare, and there have been few reports of appendiceal ganglioneuroma.

Case presentation
The patient was a 29-year-old man diagnosed with neurofibromatosis type 1 based on physical findings and his family history. During the follow-up of neurofibromatosis, computed tomography was performed to detect neurological tumors, such as neurofibromas in the brain, spinal cord, and gastrointestinal tract. Computed tomography showed a markedly thickened appendix wall, and an appendiceal tumor was suspected. Laparoscopic appendectomy was performed, and a 50 × 35 mm appendiceal submucosal tumor was resected with a negative resection margin. At histopathological examination, the tumor was diagnosed as ganglioneuroma; it showed short spindle-shaped cells and ganglion cells diffusely infiltrated into the proper muscle layer and fibrous tissue that grew around nerve cells. The patient was discharged on the 5th postoperative day without postoperative complications and was doing well at 13 months following the operation.

Conclusions
Gastrointestinal stromal tumor and neurofibroma are the most common gastrointestinal tumors associated with neurofibromatosis type 1, but ganglioneuroma of the appendix is rare. Appendiceal neurogenic tumors should be considered in patients with neurofibromatosis type 1, and surgical resection is necessary because of the risk of malignancy.
The legacy of fire: long-term changes to the forest understory from periodic burns in a New England oak-hickory forest
Authors: Borden Caroline G. , Duguid Marlyse C. , Ashton Mark S. 
Published: September 2021

AbstractBackgroundOver the last century, fire exclusion has caused dramatic structural and compositional changes to southern New England forests, highlighting the need to reintroduce fires into the historically pyrogenic landscape to study the response. We investigated the effects of a single overstory thinning and midstory removal to create an open oak-hickory woodland structure, followed by repeated prescribed burns. We hypothesized that burning would create greater floristic diversity comprising fire-tolerant woody regeneration and shade-intolerant herbaceous flora. We followed shifts in plant structure, composition, and diversity over a 23-year period, using a before-after-control-impact design with data collected once prior to burning and twice after burn treatments had begun and with soil samples collected after nearly 20 years of burning.ResultsWe observed a dense ingrowth of saplings on unburned plots that were largely absent from burned plots and a shift in midstory composition to favor mesic sweet birch (Betula lentaL.) in the unburned treatment, as opposed to the hickories (CaryaNutt. spp.) and oaks (QuercusL. spp.) that dominated the burned treatment. Burning resulted in a significantly greater density, richness, Shannon diversity, and evenness of understory vegetation (forbs, shrubs, tree seedlings). These four measures remained high on burned plots, despite a decrease in both floristic diversity and evenness on unburned plots and a reduction in unburned site-level richness. Understory composition varied significantly by year and burn treatment, with unburned plots largely characterized by shade-tolerant species while burned plots showed an enhanced abundance of heliophilic plants.ConclusionsOur results suggest that periodic burning increases nutrient microsite heterogeneity and periodically maintains greater understory light, both of which in turn increase understory plant density and diversity and cause a shift in understory composition. This study shows that repeated prescribed burns in an open New England woodland have lasting structural and compositional effects capable of restoring pre-settlement, pyrogenic vegetation patterns.