bluuebunny/crossref_metadata_2025_split huggingface dataset:
A sequentially advancing algorithm based on multi-value dynamic programming for the cut-off grade optimization in open-pit metalliferous deposits
Authors: Biswas Pritam , Sinha Rabindra Kumar , Sen Phalguni 
Published: December 2021

Abstract
In techno-economic concern, cut-off grade (COG) optimization is the key for efficient mineral liquidation from the huge metalliferous surface mining sector. In this paper, a sequentially advancing algorithm based on exact multi-value dynamic programming (MDP) has been developed to determine the optimum COG of an open-pit metalliferous deposit. The proposed COG optimization algorithm aims to overcome the limitations of straightforward classical techniques in determining the optimum COG. This discrete COG-MDP model is the first of its kind and has the novelty of dealing with the simulation of eight dynamic possibilities to achieve the maximal Net Present Value (NPV). A high-level programming language (Python) has been used to develop the computer model to deal with the complexity of handling a minimum of 500 series of dynamic variables with a precision value of 0.01% in grade bins. This model can generate results in polynomial-time from the complex mine, mill, and smelter and refinery system corresponding to various limiting conditions. The prime objective considered in the model is to optimize the COG of a metalliferous deposit. The model validation has been done using a real-life case study of an open-pit copper mine in India (Malanjkhand Copper Mine, HCL), considering the fixed yearly output of the mining, milling, and smelting and refining. In this study, the optimum COG for the Malanjkhand copper deposit has been found to be (0.33%, 0.23%, 0.52%, 0.26%, 0.27%, 0.22%, 0.24%) with a maximum NPV of ₹ (12204, 14653, 16948, 14609, 21454, 26717, 38821) million corresponding to various scenarios. The findings also show that the present value of net cash-flow grows in the early years, peaks at a specified mid-life time, and then drops as the reserve is depleted. The present value gradually hits zero after the project’s life cycle, confirming the typical pattern of other mining firms.
Bimetallic Chitosanstabilized Nanoparticles Ag/Cu, Ag/Co with Fungicidal Properties
Authors: Vokhidova Noira , Rashidova S. Sh. 
Published: December 2021

Abstract
Synthesized chitosan stabilized bimetallic nanoparticles Ag/Cu and Ag/Co. It was found that the size and shape of bimetallic nanoparticles can be controlled by varying the concentration of the reducing agent – NaBH4 and the molar ratios of metal ions.Established that an increase in the concentration of the reducing agent, as well as metal ions, promote the formation of fibrillar nanoparticles. It was found that solutions of the synthesized samples effectively suppress the growth and development of the phytopathogen - Fusarium oxysporium forming a sterile zone from 13.3 to 36 mm.
Fabrication of Methylene Blue Loaded Ovalbumin/Polypyrrole Nanoparticles for Enhanced Phototherapy-Triggered Antitumor Immune Activation
Authors: Xu Xiao , Mao Huafen , Wu Yunchao , Liu Suwan , Liu Jingjin , Li Qianzhe , Yang Mengyu , Zhu Jinqian , Zou Shengqiang , Du Fengyi 
Published: December 2021

Abstract
Phototherapy-triggered immunogenic cell death (ICD) hardly elicit robust antitumor immune response partially due to low antigen exposure and inefficient antigen presentation. To address these issues, we developed a novel methylene blue loaded ovalbumin/ polypyrrole nanoparticles (MB@OVA/PPY NPs) via oxidative polymerization and π-π stacking reaction. The as-prepared MB@OVA/PPY NPs with outstanding photothermal conversion efficiency (38%) and photodynamic property could be readily internalized into cytoplasm and accumulated in lysosome and mitochondria. Upon 808 nm and 660 nm laser irradiation, the MB@OVA/PPY NPs not only ablated the tumor cells by inducing local hyperthermia, but also damaged residual tumor cells by generating a large amount of reactive oxygen species (ROS), finally triggered the release of large amount of damage associated molecular patterns (DAMPs). Moreover, the MB@OVA/PPY NPs synergized with DAMPs promoted thematuration and improved antigen presentation ability of DCs in virto and vivo. This work demonstrated that the MB@OVA/PPY NPs could be used as effective nanotherapeutic agents for eliminating the solid tumor and triggering powerful antitumor immune response.
Sequence Analysis of Several Ancient River Blocking Events in the Suwalong Reach of the Upper Jinsha River Based on Multidisciplinary Approaches, SE Tibetan Plateau
Authors: Zhang Yiwei , Chen Jianping , Wang Qing , Tan Chun , Li Yongchao , Song Shengyuan , Cao Chen , Gu Feifan 
Published: December 2021

Abstract
The temporary or permanent river blocking event caused by mass movement usually occurs on steep terrain. With the increase of mountain population and land use pressure and the construction of water conservancy and hydropower projects, river blocking event has gradually attracted people’s attention and understanding. The study area (Wangdalong-Gangda reach) is located in the upper reaches of the Jinsha River and the southeast edge of the Qinghai-Tibet Plateau. Affected by strong tectonic activity in the Jinsha River suture zone and the rapid uplift of the Tibetan Plateau, in the past 6000 years, there have been at least five obvious river blocking events in the reach of about 30 km in the study area. The number and density are very rare. Combined with the field investigation, indoor interpretation, laboratory tests, optically stimulated luminescence (OSL) dating, SBAS-InSAR and previous studies, multidisciplinary approaches are used to systematically summarize the analysis methods and further the understanding of one river blocking event and multiple river blocking events from difference perspectives. Especially in multiple river blocking events, we could get the wrong results, even the opposite conclusion if interaction is not considered. Through this study, the general method of analyzing the river blocking event and the problems that should be paid attention to in sampling are given, and relatively reliable historical results of river blocking events are obtained. This method has extensive applicability to the identification and analysis of river blocking events in other areas.
KMT2C is Potential Prognostic Biomarker and its Immune Regulating Roles in Pan-Cancer: A Comprehensive Analysis
Authors: Wei Guangnan , Zhang Yuchen , Zhuang Hongkai , Li Yingzi , Ren Chongyang , Wen Lingzhu , Lin Jiali , Dai Danian , Chen Bo , Liao Ning 
Published: December 2021

Abstract
Background: A member of histone lysine methyltransferases subfamily, The histone 3 lysine 4 (H3K4) monomethylase KMT2C, has mutations across many cancer types. However, the role of KMT2C in different cancers and its correlation with tumor infiltration and immune therapy indicators remain unknown.Method: Expression and mutation information of KMT2C has been analyzed through the Genotype-Tissue Expression (GTEx), The Cancer Genome Atlas (TCGA) Cancer Cell Line Encyclopedia (CCLE) and International Cancer Genome Consortium (ICGC) database in our study. Prognostic value of KMT2C was evaluated via univariate survival analysisand expression detection in different cancer cells. Result: Survival analysis showed that high expression of KMT2C in some cancer type may be a indication of better outcome, while in other cancer like UVM, patient with high expression of KMT2C suffered from early recurrence. Further, we found there is a strongly link between KMT2C expression and immune cells infiltration, mutation indicators through analysising in the Tumor Immune Evaluation Resource (TIMER) database. Conclusion: The bioinformatics analysis here deliver us a message that KMT2C might be a good molecular biomarker for prognostic and therapeutic evaluation in specific cancer types.
Development of an SNP Marker Set for Marker-Assisted Backcrossing Using Genotyping-By-Sequencing in Tetraploid Perilla
Authors: Kang Yun-Joo , Lee Bo-Mi , Kim Jangmi , Nam Moon , Lee Myoung-Hee , Lee Keunpyo , Kim Tae-Ho , Jo Sunghwan , Lee Jeong-Hee 
Published: December 2021

Abstract
High-quality molecular markers are essential for marker-assisted selection to accelerate breeding progress. Compared with diploid species, recently diverged polyploid crop species tend to have highly similar homeologous subgenomes, which is expected to limit the development of broadly applicable locus-specific single-nucleotide polymorphism (SNP) assays. Furthermore, it is particularly challenging to make genome-wide marker sets for species that lack a reference genome. Here, we report the development of a genome-wide set of kompetitive allele specific PCR (KASP) markers for marker-assisted recurrent selection (MARS) in the tetraploid minor crop perilla. To find locus-specific SNP markers across the perilla genome, we used genotyping-by-sequencing (GBS) to construct linkage maps of two F2 populations. The two resulting high-resolution linkage maps comprised 2,326 and 2,454 SNP markers that spanned a total genetic distance of 2,133 cM across 16 linkage groups and 2,169 cM across 21 linkage groups, respectively. We then obtained a final genetic map consisting of 22 linkage groups with 1,123 common markers from the two genetic maps. We selected 96 genome-wide markers for MARS and confirmed the accuracy of markers in the two F2 populations using a high-throughput Fluidigm system. We confirmed that 91.8% of the SNP genotyping results from the Fluidigm assay were the same as the results obtained through GBS. These results provide a foundation for marker-assisted backcrossing and the development of new varieties of perilla.
Assessing Health Risk Associated with Uranium in Rietspruit Water, Far West Rand Goldfield, South Africa
Authors: Raji Iyioluwa Busuyi , Hoffmann Emile , Erasmus Ewald , Ngie Adeline , Winde Frank 
Published: December 2021

Abstract
Uranium, U, as a toxic element has detrimental radiological and chemical impacts on human health when ingested at elevated concentration in water. The establishment of gold mining operations upstream to the Rietspruit is assumed to be a source of uranium into this water body which flows through residential and agricultural land. Water samples from the stream were collected and analysed using inductively coupled plasma mass spectrometry to determine the concentration of U. Uranium concentrations above South African regulatory limit were recorded in the stream water which is used for domestic, agricultural and religious activities as observed during field visits. Results from calculations showed that the maximum annual effective dose within this mining environment was above 1 mSv/year for all age groups. Furthermore, the probability of radiological risk is lower than the chemical risk which is due to the ingestion of U in the Rietspruit water. The chemical risk analysis showed that within 24 km of this mining environment, stream users are in danger of U chemical toxins therefore, it is important to educate the stream users about their health risk of U and recommend adequate interventions by relevant government agencies to this effect.
Antibacterial and Antibiofilm Activity of Mercaptophenol Functionalized-Gold Nanorods Against a Clinical Isolate of Methicillin-Resistant Staphylococcus Aureus
Authors: Eltai Nahla O. , Mahmoud Nouf , Zakaria Zain Z. , Abdelrahman Hana , Moustafa Ala-Eddin Al , Al-Asmakh Maha 
Published: December 2021

Abstract
Gold nanorods (AuNRs) were synthesized and surface functionalized with 4-mercaptophenol (4-MPH) ligand. The surface-functionalized AuNRs, 4-MPH-AuNRs, were characterized by UV-Vis spectroscopy, dynamic light scattering (DLS), transmission electron microscopy (TEM) imaging, zeta potential, and Fourier-Transformed Infrared (FTIR) spectroscopy. The antibacterial and antibiofilm activities of 4-MPH-AuNRs were evaluated against a clinical isolate of Methicillin-Resistant Staphylococcus aureus (MRSA). The results indicate that the surface-modified nanorods, 4-MPH-AuNRs, exhibit a bactericidal activity with a minimum inhibitory concentration (MIC) of ~6.25 \(\mu\)g/ml against a planktonic suspension of MRSA. Furthermore, 4-MPH-AuNRs resulted in 2-3 log-cycle reduction of MRSA biofilm viable count over a concentration range of 100-4.0 \(\mu\)g/ml. The bacterial uptake of surface-modified nanorods was investigated by inductively coupled plasma-optical emission spectroscopy (ICP-OES) and scanning electron microscopy (SEM) imaging; the results reveal that ~27% of the nanorods were internalized into the bacterial cells after 6 hrs of exposure. SEM imaging revealed a significant accumulation of the nanorods at the bacterial cell wall and a possible cellular internalization. Thus, 4-MPH-AuNRs can be considered a potential novel antibacterial agent, particularly against resistant MRSA strain biofilm.
Maize (Zea Mays L.) Genotypes Induce the Changes of Rhizosphere Microbial Communities
Authors: Li Yuehan , Qu Zheng , Xu Weihui , Chen Wenjing , Hu Yunlong , Wang Zhigang 
Published: December 2021

Abstract
Plant-microbe interactions affect ecosystem function, and plant species influence relevant microorganisms. However, the different genotypes of maize that shape the structure and function of the rhizosphere microbial community remain poorly investigated. During this study, the structures of the rhizosphere microbial community among three genotypes of maize were analyzed at the seedling and maturity stages using high-throughput sequencing and bioinformatics analysis. The results demonstrated that Tiannuozao 60 (N) showed higher bacterial and fungal diversity in both periods, while Junlong1217 (QZ) and Fujitai519 (ZL) had lower diversity. The bacterial community structure among the three varieties was significantly different; however, fewer differences were found in the fungal community. The bacterial community composition of N and QZ was similar yet different from ZL at the seedling stage. The bacterial networks of the three cultivars were more complex than the fungal networks, and the networks of the mature stages were more complex than those of the seedling stages, while the opposite was true for the fungi. FAPROTAX functional and FUNGuild functional predictions revealed that different varieties of maize were different in functional abundance at the genus level, and these differences were related to breeding characteristics. This study suggested that different maize genotypes regulated the rhizosphere bacterial and fungal communities, which would help guide practices.
Association of Elevated Non-HDL-C and LDL-C with Carotid Atherosclerosis among Tibetans Living at High Altitudes: A Cross-sectional Study
Authors: Hu Xianjin , Zhang Xin , Gou Qiling , Ye Runyu , Zhang Zhipeng , Li Xinran , Chen Xiaoping 
Published: December 2021

Abstract
Background: Hypoxic circumstances impair endothelial function and may contribute to carotid atherosclerosis. In high-altitude areas, there is a scarcity of data on the correlation between lipid particles and carotid atherosclerosis. Methods: A total of 587 patients who underwent carotid artery ultrasound and met the inclusion and exclusion criteria were enrolled in our cross-sectional study. All participants resided in Luhuo County, Ganzi Tibetan Autonomous Prefecture, Sichuan Province, China (mean altitude: 3,860 meters). We used questionnaires, physical examination, blood sample testing, and ultrasound in our investigation. Spearman correlation analysis and multiple linear regression analysis were performed to explore the association between lipid particles and carotid atherosclerosis. We compared the disparity between lipid particles in predicting atherosclerosis using the receiver operator characteristic curve.Results: We found a statistically significant association between lipid particles and carotid atherosclerosis. After adjustment for certain variables, including age, gender, mean arterial pressure, and fasting blood glucose, we discovered that non-high-density lipoprotein-cholesterol (non-HDL-C) was a risk factor for carotid intima-media thickness (β = 0.012, p = 0.032) but not for low-density lipoprotein cholesterol (LDL-C) (p = 0.073). In terms of lifestyle, non-HDL-C was also found to be a risk factor for atherosclerosis independent of cigarette smoking and vegetarian (β = 0.012, p = 0.049). The optimal cut-off value of non-HDL-C was 3.625 mmol/L in predicting carotid plaques.Conclusions: Among Tibetans living in high-altitude areas, non-HDL-C is a better biomarker than LDL-C for carotid artery atherosclerosis independent of conventional risk factors.
Long Term Protective Immunity to Goatpox in Goats After Single Immunization with a Live Attenuated Goatpox Vaccine
Authors: V Bhanuprakash , Hosamani Madhusudan , Venkatesan Gnanavel , Singh Raj Kumar 
Published: December 2021

Abstract
In this study, duration of immunity following single shot vaccination using an attenuated goatpox vaccine (GTPV/Uttarkashi/1978) was evaluated in sero-negative kids for 52 months. Long term immunity was evaluated by clinical protection upon virulent virus challenge and serum neutralization assay applied for serum samples. Rise in level of GTPV specific antibodies was found to be maximum on 21 days post vaccination, which was maintained between 1 and 2 years of immunization with steady decline. Upon virulent virus challenge on 21 days, 12, 24, 42 and 52 months post vaccination, protection in all vaccinated animals was evident, whereas, control animals developed severe clinical disease. This is for the first time that long term immunity of a live goatpox vaccine has been investigated up to 52 months of post-vaccination in goats and it has immense potential in controlling and eradicating goatpox from an enzootic situation.
Risk Factors for Lymph Node Metastasis in Clinical Stage IA3 Lung Adenocarcinoma Patients
Authors: Zheng Yuan-Liang , Sheng Ju , Huang Ri-Sheng , Zhao Jun 
Published: December 2021

Abstract
Background: lymph node metastasis is a poor prognostic factor for lung cancer; however, the risk of lymph node metastasis has not been clarified yet, so it is controversial to conduct systematic lymph node dissection for early lung cancer. Therefore, this study aimed to focus on analyzing the predictive factors for lymph node metastasis in patients with clinical stage IA3 lung adenocarcinoma.Methods: Our study group retrospectively analyzed all surgical patients admitted to our hospital from January 1, 2017 to June 2021, and these patients were considered having stage IA3 lung adenocarcinoma. A total of 334 patients underwent lobectomy combined with systematic lymph node dissection. Univariate and multivariate logistic regression analysis were adopted to predict the risk factors of lymph node metastasis.Results: Among the 334 patients eligible for this study, the overall mediastinal lymph node metastasis rate was 15.27%. There were 45 cases of N1 metastasis and 11 cases of N2 metastasis, 5 cases had both N1 and N2 metastasis at the same time. The patients were divided into three groups according to consolidation tumor ratio (CTR) values (<0.25, 0.25-0.5, >0.5). The lymph node metastasis rates in each CTR group were 1.8% (2/112), 11.7% (17/145) and 41.6% (32/77), respectively. The mediastinal lymph node metastasis rate in patients with carcinoembryonic antigen (CEA>5ng/ml) was 57.89% (22/38). The receiver operating characteristic curve (ROC) showed that the area under the curve (AUC) of CTR, pathological type and CEA were 0.790 [95% confidence interval (CI): 0.727 – 0.853，P<0.001]; 0.800（95% CI:0.735–0.865，P<0.001）;0.682（95% CI: 0.591–0.773, P<0.001）;respectively. Multivariate regression analysis showed that these listed factors were significantly correlated with lymph node metastasis of clinical stage IA3 lung adenocarcinoma: CEA [Odds Ratio (OR)=3.05, P=0.016], CTR 0.25 to 0.5 (OR=14.12, P<0.017), CTR>0.5 (OR=7.75, P=0.015), micropapillary adenocarcinoma (OR=15.704, P<0.001), and solid adenocarcinoma (OR=8.971, P=0.001).Conclusions: CEA (>5ng/ml), histologic subtype and CTR (>0.25) are important predictors of lymph node metastasis in clinical stage IA3 lung adenocarcinoma, systematic lymph node dissection should be the prior choice for patients with clinical stage IA3 incorporated with risk factors. The lymph node dissection method in stage IA3 should be alternative from those in stage IA1 and IA2.
Dysregulated Spliceosome Gene Expression May Be a Common Process in Brains of Neurological and Psychiatric Disorders
Authors: Xia Cuihua , Dai Rujia , Yu Jing , Zhang Chunling , Wong Ma-li , Liu Chunyu 
Published: December 2021

Abstract
Alternative splicing (AS) contributes to the increased cellular and functional tissue complexity that is substantial in the brain. AS is tightly regulated because it is critical to many biological processes. Defective splicing is observed in several neurological and psychiatric disorders. While exonic mutations usually affect the splicing of an individual RNA, mutations in the splicing factors (components of spliceosome) frequently produce widespread disruption in the processing of many precursor-mRNAs. Thus, we tested the hypotheses that expression changes of spliceosome genes may be a common process and shared splicing pathways may be involved in complex polygenic brain disorders. We searched for expression changes of spliceosome-related genes (SGs) using a transcriptome database of several brain regions in 6 neurological and psychiatric disorders, namely Alzheimer’s disease, and autism spectrum, bipolar and major depressive disorder, Parkinson’s disease, and schizophrenia. Out of 255 SGs detected in brain, 138 showed excessive, significant changes in one or more of these disorders. Dysregulation of 10 SGs was shared in 4 disorders, and they were mostly downregulated. Six associated pathways were over-represented in all 6 disorders, including the major and the minor mRNA splicing pathways and RNA metabolism. Therefore, we found that aberrations in the mRNA splicing process may be a common trajectory to many complex brain disorders involving the spliceosome complex.
High Expression of H2BC12 Predicts Poor Survival Outcome of Low-Grade Gliomas: A Study Based on TCGA Data
Authors: Xiao Yilei , Xing Zhaoquan , Li Mengyou , Li Xin , Wang Ding , Dong Zhaogang 
Published: December 2021

Abstract
Purpose

Low-grade gliomas (LGG) have highly variable clinical behaviors, with a high incidence of disease progression as 70% within ten years. Regardless of treatment combining surgery and radiotherapy or chemotherapy, LGG is still associated with adverse survival outcomes. Therefore, our study was performed to satisfy the increasing demand of novel sensitive biomarkers and therapeutic targets in treatment and diagnosis of LGG.
Methods

The TCGA data set was used to examine the relationship between H2BC12 expression and clinical pathologic characteristics. The significance of H2BC12 expression in prognosis was also investigated. In addition, H2BC12 expression-related pathways were enriched by gene set enrichment analysis (GSEA). Association analysis of H2BC12 gene expression and immune infiltration was performed by single sample gene set enrichment analysis (ssGSEA).
Results

Significantly up-regulated expression of H2BC12 mRNA was found in LGG tissue when compared to normal tissue and was proven to be diagnostic (have diagnostic significance) for LGG. In the meantime, high H2BC12 levels were associated with WHO grade, IDH status, 1p/19q codeletion, primary therapy outcome and histological type of LGG, and additionally, prognostic for adverse survival outcomes. In the multivariate analysis, high H2BC12 levels were identified to be an independent predictor for poor survival outcomes of LGG patients. Pathways in cancer, signaling by Wnt or PI3K-AKT signaling pathway, DNA repair, cellular senescence and DNA double strand break repair were differentially activated in the phenotype that positively associated with H2BC12.
Conclusion

H2BC12 is a promising biomarker for the diagnosis and prognosis of LGG.