bluuebunny/crossref_metadata_2025_split huggingface dataset:
ChAd155-RSV vaccine is immunogenic and efficacious against bovine RSV infection-induced disease in young calves
Authors: de Jong Rineke , Stockhofe-Zurwieden Norbert , Bonsing Judith , Wang Kai-fen , Vandepaer Sarah , Bouzya Badiaa , Toussaint Jean-François , Dieussaert Ilse , Song Haifeng , Steff Ann-Muriel 
Published: October 2021

Abstract
Respiratory syncytial virus (RSV) infection causes a substantial lower respiratory-tract disease burden in infants, and is a global priority for vaccine development. We evaluated the immunogenicity, safety and efficacy of a chimpanzee adenovirus (ChAd)-based vaccine candidate, ChAd155-RSV, in a bovine RSV (bRSV) challenge model. This model closely reproduces the pathogenesis/clinical manifestations of severe pediatric RSV disease. In seronegative calves, ChAd155-RSV elicited robust neutralizing antibody responses against human RSV. Two doses protected calves from clinical symptoms/lung pathological changes, and reduced nasal/lung virus loads after both a short (4-week) and a long (16-week) interval between the last immunization and a subsequent bRSV challenge. The one-dose regimen also conferred near-complete or significant protection after the short-term or long-term intervals before challenge, respectively. Importantly, immunized calves presented no clinical signs of enhanced respiratory disease. Collectively, the data supported the development of ChAd155-RSV as an RSV vaccine candidate for infants.
Are Patients with Cardiovascular Disease at Higher Risk for Postoperative Complications following Total Hip or Total Knee Replacements? A Retrospective Cohort Study
Authors: Orland Mark , Siva Nandini Rajaram , Barragan Diego , Sood Anshum , Gonzalez Mark H 
Published: October 2021

Abstract
Background: Cardiovascular disease affects 121.5 million Americans, or 48% of the adult US population and is the leading cause of death in the United States. The demand for total joint replacement is expected to increase due to an aging population and advances in technology. Given the prevalence of cardiovascular disease and the expected increase in total joint replacement procedures, we sought to examine whether cardiovascular disease was associated with increased postoperative complications in patients undergoing total hip and knee replacements.Methods:CPT codes were used to investigate the American College of Surgeons National Surgical Quality Improvement Program (ACS-NSQIP) database for patients who underwent total hip or knee replacements from 2010 to 2017. Patients were classified as having cardiovascular disease as per the American Health Association guidelines. Patient samples with all possible covariates were included in a multivariate logistic regression analysis. A patient sample size of 119,076 for total hip and 189,297 for total knee replacement was analyzed.Results: Patients with cardiovascular disease were found to have a statistically significant increased risk for the following postoperative complications: sepsis, wound complications, pneumonia, urinary tract infection, myocardial infarction, unplanned intubation, and cardiac arrest. Conclusions: Patients with cardiovascular disease undergoing a total hip or knee replacement have increased risk for wound, infectious and cardiovascular-associated postoperative complications. This finding necessitates the need for more frequent and sooner postoperative visits, a stringent cardiovascular preoperative evaluation, and patient education on the increased postoperative risks of joint replacements to lower the incidence of complications.
MiR-425-5p Regulates Glucagon-like Peptide-1 Production and Affects Blood Glucose Levels in High-fat Diet-fed Mice
Authors: Wei Lirui , Zhao Xuenan , Guo Feng , Huang Fengjiao , Zhao Yanyan , Wu Lina , Liu Yanling , Ma Xiaojun , Chen Linlin , Li Jing , Ji Linlin , Xiao Ya , Ding Chengzhi , Wang Jiao 
Published: October 2021

Abstract
BackgroundIn modern society, obesity has become a global problem with resulting in metabolic disorders and poses high risk for type 2 diabetes mellitus (T2DM). The glucagon-like peptide-1 (GLP-1) has been taken as an effective drug for the therapy of T2DM and obesity. In the present study, the regulatory roles and molecular mechanisms of miR-425-5p in GLP-1 secretion in high-fat diet (HFD)-induced diabetic mice were explored. MethodsOral glucose tolerance test and insulin tolerance test were performed to assess glucose metabolism and GLP-1 and LPS levels. Quantitative real time polymerase chain reaction (qRT-PCR) was employed to detect the expression of LPS, GLP-1, GLP-1 receptors, miR-425-5p, phosphatase and tensin homology (PTEN), proglucagon, p65 and β-catenin. Western blot was performed to determine the expression of proglucagon, p65, β-catenin and PTEN. ResultsThe results showed that plasma GLP-1 level was negatively correlated with plasma LPS level in HFD-fed mice, and miR-425-5p expression and LPS level were up-regulated in the ileal fluid compared with control groups. LPS injection boosted miR-425-5p expression in ileum. MiR-425-5p ameliorated glucose intolerance and insulin resistance in HFD-fed mice by increasing GLP-1 secretion. Furthermore, p65 protein level in the cytoplasmic and nuclear in the ileum of HFD-fed mice was increased compared with the control group. MiR-425-5p agomir elevated nuclear β-catenin protein level, but reduced PTEN protein level in HFD-fed mice compared with HFD-fed mice treated with the miR-425-5p antagomir. ConclusionsOur results suggest that miR-425-5p promotes GLP-1 secretion and improves glucose tolerance and insulin resistance in high-fat diet-fed mice.
Association between Long noncoding RNA rs944289 and rs7990916 polymorphisms and the risk of colorectal cancer in a Chinese population
Authors: Wang Yan , Qiu Zhiyuan , Tian Guangyu , Zhu Qianqian , Zhang Zhao , Qin Rong , Peng Yong , Tang Weifeng , Zhang Sheng , Xi Yan 
Published: October 2021

Abstract
Long non-coding RNAs (LncRNAs) play vital roles in the tumorigenesis of many cancers. Single nucleotide polymorphisms (SNPs) of the lncRNA also play vital roles in tumorigenesis. We explored lncRNA rs944289 and rs7990916 polymorphisms and analyzed the relationship between these lncRNA polymorphisms with the colorectal cancer (CRC) risk in a Chinese population. We recruited 1,003 CRC patients from the Affiliated People’s Hospital of Jiangsu University and the Fujian Medical University Union Hospital from October 2014 to August 2017. Genomic DNA was extracted using a DNA Kit from lymphocytes of peripheral blood and the genotyping was performed with a SNPscan method. We found that the rs944289 TT homozygote was associated with the decreased CRC risk. However, there was no statistically significant association between lncRNA rs7990916 polymorphism and the CRC risk. LncRNA rs944289 TT decreased the CRC risk in the subgroup of female by logistic regression, male, age≥61, without alcohol intake, smoking and BMI≥24. The subgroup analysis revealed that lncRNA rs7990916 was not associated with the CRC risk except for age<61. Logistic regression analysis revealed that the lncRNA rs944289 TT homozygote was associated with the increased risk of rectum cancer (TT vs. CC+CT: adjusted OR=1.29, 95% CI: 1.10-1.66, P=0.041) or colon cancer. In summary, we proved that the lncRNA rs944289 might be significantly related to the decreased CRC risk in the Chinese Han populations and lncRNA rs7990916 was not associated with the CRC risk except for patients of age<61. In the future, studies with larger samples should be conducted to validate our results.
Biochemical And Genetic Characteristics of Patients With Primary Carnitine Deficiency Identified Through Newborn Screening
Authors: Lin Yiming , Lin Bangbang , Chen Yanru , Zheng Zhenzhu , Fu Qingliu , Lin Weihua , Zhang Weifeng 
Published: October 2021

Abstract
Background: Primary carnitine deficiency (PCD) is an autosomal recessive disorder of the carnitine transportation that leads to impaired fatty acid oxidation. Large-scale studies on newborn screening (NBS) for PCD are limited. This study aimed to investigate the biochemical and genetic characteristics of patients with PCD detected by NBS.Results: A total of 548,247 newborns were screened for PCD between January 2014 and June 2021, 1714 newborns had low free carnitine (C0) levels were called back and forty-nine patients were diagnosed with PCD. The latest incidence rate in Quanzhou, China was estimated to be 1 in 11,189 newborns. NBS results showed that all patients had varying degrees of decreased C0 levels, while seven patients exhibited normal C0 levels during recall review. All patients harbored biallelic pathogenic variants in the SLC22A5 gene. Nineteen distinct SLC22A5 variants were detected in the 49 patients, most of the detected variants were clustered in exons 1, 4, and 7. The top eight variants together had an allele frequency of 86.73%. The most common variant was c.760C>T (p.R254*) with an allele frequency of 31.63%, followed by c.51C>G (p.F17L) (17.35%) and c.1400C>G (p.S467C) (16.33%). The C0 level of patients with N/N genotype was significantly lower than that of M/M group. The C0 level of patients with genotypes of R254*/R254* and R254*/F17L were far lower than patients with genotype of R254*/S467C.Conclusions: This study presented more than 500,000 NBS data with the latest incidence of 1:11,189 in Quanzhou area. The SLC22A5 variant spectrum in the selected southern Chinese population was updated. Patients with null variants were associated with low C0 levels. It is necessary to combine genetic testing to improve screening efficiency due to PCD patients may have normal C0 levels during NBS and recall review.
Long Non-coding RNA ADAMTS9-AS2 Suppresses Biological Functions of Ovarian Cancer Cells Through Recruiting DNMTl and Up-regulating SEPT6
Authors: Wang Juan , Ding Hongmei , Zhou Jinhua , Shen Fangrong , Li Yan , Chen Youguo 
Published: October 2021

Abstract
Objective: The mechanism of long non-coding RNA ADAM metallopeptidase with thrombospondin type 1 motif, 9 antisense RNA 2 (ADAMTS9-AS2) has been insufficiently explored in ovarian cancer (OC). Here, the action of ADAMTS9-AS2 in OC with the involvement of DNA methyhransferase l (DNMT1) and septin 6 (SEPT6) was explored. Methods: Epithelial OC tissue and ovarian epithelial tissue specimens were collected to test ADAMTS9-AS2, DNMT1 and SEPT6 expression. An appropriate cell line was screened out and transfected with highly expressed ADAMTS9-AS2 or SEPT6 or lowly expressed DNMT1 to detect their roles in biological functions of OC cells. Tumorigenesis of OC cells in nude mice was projected to validate cell experiment results. The interactions between ADAMTS9-AS2 with DNMT1/SEPT6 were tested. Results: Elevated DNMT1 and reduced ADAMTS9-AS2 and SEPT6 were detected in OC. ADAMTS9-AS2 bound to both DNMT1 and SEPT6. Restoring ADAMTS9-AS2 or SEPT6 or depleting DNMT1 diminished OC cell viability, invasion and migration, arrested cell cycle and accelerated apoptosis in vitro, and repressed tumor growth in vivo. Conclusion: Collectively, ADAMTS9-AS2 fights against OC through recruiting DNMT1 and up-regulating SEPT6.
Three-dimensional Finite Element Analysis of the Effect of Alveolar Cleft Bone Graft on the Maxillofacial Biomechanical Stabilities of Unilateral Complete Cleft Lip and Palate
Authors: Tian Tao , Huang Han-yao , Wang Wei , Shi Bing , Zheng Qian , Li Cheng-hao 
Published: October 2021

Abstract
Background: The objective was to clarify the effect of alveolar cleft bone graft on maxillofacial biomechanical stabilities, the key areas when bone grafting and in which should be supplemented with bone graft once bone resorption occurred in UCCLP (Unilateral Complete Cleft Lip and Palate).Methods: Maxillofacial CAD (Computer Aided Design) models of non-bone graft and full maxilla cleft, full alveolar cleft bone graft, bone graft in other sites of the alveolar cleft were acquired by processing the UCCLP maxillofacial CT data in three-dimensional modeling softwares. The maxillofacial bone equivalent (EQV) stresses and bone suture EQV strains under occlusal states were obtained in the finite element analysis software.Results: Under corresponding occlusal states, the EQV stresses of maxilla, pterygoid process of sphenoid bone on the corresponding side and anterior alveolar arch on the non-cleft side were higher than other maxillofacial bones, the EQV strains of nasomaxillary, zygomaticomaxillary and pterygomaxillary suture on the corresponding side were higher than other maxillofacial bone sutures. The mean EQV strains of nasal raphe, the maximum EQV stresses of posterior alveolar arch on the non-cleft side, the mean and maximum EQV strains of nasomaxillary suture on the non-cleft side in full alveolar cleft bone graft model were all significantly lower than those in non-bone graft model. The mean EQV stresses of bilateral anterior alveolar arches, the maximum EQV stresses of maxilla and its alveolar arch on the cleft side in the model with bone graft in lower 1/3 of the alveolar cleft were significantly higher than those in full alveolar cleft bone graft model.Conclusions: For UCCLP, bilateral maxillae, pterygoid processes of sphenoid bones and nasomaxillary, zygomaticomaxillary, pterygomaxillary sutures, anterior alveolar arch on the non-cleft side are the main occlusal load bearing structures before and after alveolar cleft bone graft. Alveolar cleft bone graft mainly affects biomechanical stabilities of nasal raphe and posterior alveolar arch, nasomaxillary suture on the non-cleft side. The areas near nasal floor and in the middle of the alveolar cleft are the key sites when bone grafting, and should be supplemented with bone graft when the bone resorbed in these areas.
Screening of Antibacterial Compounds With Novel Structure From The FDA Approved Drugs Using Machine Learning Methods
Authors: Li Wen-Xing , Tong Xin , Yang Peng-Peng , Zheng Yang , Liang Ji-Hao , Li Gong-Hua , Guan Dao-Gang , Dai Shao-Xing 
Published: October 2021

Abstract
Background: Due to the lack of new antibiotics in recent years, bacterial resistance has increasingly become a serious problem globally. The aim of this study is to construct an antibacterial compound predictor using machine learning methods to screen potential antibacterial drugs.Methods: Active and inactive antibacterial compounds were acquired from the ChEMBL and PubChem database, which were used to construct benchmark datasets. The antibacterial compound predictor is constructed using the support vector machine (SVM), random forest (RF), and multi-layer perception (MLP) methods. We predicted the antibacterial activity of the Food and Drug Administration (FDA) approved drugs in the DrugBank database and screened novel antibacterial drugs through structural similarity analysis.Results: In the initial screen process, the results suggested that the benchmark dataset based on FP2 molecular fingerprints, along with the SVM, RF, and MLP methods showed excellent prediction performance (mean AUC > 0.9 for all models). Using the combination of these three models, a total of 957 potential antibacterial drugs were predicted. Most of the predicted drugs showed low structural similarities compared with the FDA approved antibacterial drugs. We finally screened 9 predicted antibacterial drugs with novel structures including 2 anti-tumor drugs (cyclophosphamide and ifosfamide), 2 ophthalmic drugs (apraclonidine and echothiophate) and 5 anesthetics (desflurane, enflurane, isoflurane, methoxyflurane, and sevoflurane).Conclusions: This study provides a new insight for predicting antibacterial compounds with novel structures by using FDA approved drugs. The predicted compounds with novel structures are worthy of further experimental verification in the future.
CrossFit® Open Performance is Affected by the Nature of Past Competition Experiences
Authors: Mangine Gerald T , McDougle Jacob M. 
Published: October 2021

Abstract
Purpose: To examine the relationships between past competition performances and 2020 CrossFit® Open (CFO) performance. Methods: A random selection from the top one thousand athletes (n = 220, 28.5 ± 4.4 years, 178 ± 7 cm, 87.5 ± 10.2 kg) were selected for this study. Overall and weekly performances (including ranks and scores) of the 2020 CFO, as well as overall ranks from previous CFO, regional, and Games™ competitions, were recorded from their publicly available online profile. The highest, lowest, average, and standard deviation (SD) of past rankings, as well as participation statistics (i.e., years since first appearance, total and consecutive appearances, and participation rate), were calculated for each competition stage. Relationships were then assessed between 2020 CFO performance and all past competition experience variables by calculating Kendall’s tau (τ) correlation coefficients and Bayes factors (BF10). Results: Overall and weekly ranking of the 2020 CFO was extremely favored (p < 0.001, BF10 > 100) to be related to the athlete’s highest previous CFO rank (τ = 0.26 – 0.39) and individual regional appearances (τ = –0.26 to –0.34), as well as individual Games™ appearances (overall and for weeks 1, 3, and 4; τ = –0.20 to –0.22, p < 0.001, BF10 > 100). Evidence for all other significant relationships ranged from moderate to very strong (p < 0.05, BF10 = 3 – 100) and varied among specific 2020 CFO workouts. Few associations were noted for team competition experience, and these were generally limited to Games™ appearances (τ = –0.12 to –0.18, p < 0.05, BF10 = 3.3 – 100). Conclusions: Although specific relationships were found between 2020 CFO performance and individual appearances at regional and Games™ competitions, the most consistent relationships were seen with participation and ranking in past CFO competitions.
A Pediatric BCI Program for Children With Severe Neurological Disabilities: Thematic Analysis of Family Perspectives
Authors: Jadavji Zeanna , Zewdie Ephrem , McDonough Meghan , Kelly Dion , Kinney-Lang Eli , Kirton Adam 
Published: October 2021

Abstract
Background: Children with severe physical disabilities and no expressive communication have few options for interaction and engagement with the world around them. There is a need for new technologies and programs dedicated to this population and brain computer interface (BCI) can provide new opportunities for independence. We established a novel, family-centered, clinical BCI program for cognitively capable children with severe disability. Here, we aimed to understand the potential impact on participants from the perspective of families to help guide program development and future modifications. Methods: Children in the program were aged 6-16 years with severe physical (non-ambulatory, minimal hand use) and communication disabilities and estimated grade 1 cognitive capacity. Parents and caregivers of participants were invited to complete semi-structured interviews to discuss their impressions and opinions of the program. Children were invited to complete a simpler “yes”/ “no” questionnaire about their experience and interests. Thematic analysis was performed according to established methods. Results: Seven parents/caregivers and 4 children from 6 families with nearly 300 hours of experience with the BCI program participated. Thematic analysis revealed that despite limited initial BCI knowledge, the program elicited positive emotional responses including feelings of enhanced independence, inclusion, and recognition for parents and children. Families were interested in contributing to BCI development to enhance their child’s future. Conclusions: Clinical BCI programs may have positive impacts on children with disabilities and their families. Continuous engagement of parents and children is imperative to create programs and technologies that will meet the needs of this population. Trial registration: The current study was approved by the University of Calgary Research Ethics Board, study ID:REB15-2567.
Construction of Web-GIS in San'in region for integrating geophysical survey data with geotechnical information
Authors: Nishimura Isamu , Noguchi Tatsuya , Kagawa Takao 
Published: October 2021

Abstract
Constructing a database of information on geotechnical information, such as geophysical survey results and borehole data, and sharing it among researchers and practitioners will be useful for the development of subsurface research and the prevention of disasters such as earthquakes and landslides. In earthquake disaster prevention, geotechnical information is particularly important for strong ground motion prediction. The geotechnical information includes analysis results based on geophysical surveys and seismic observations, and borehole data. These databases can be displayed on a map using GIS, and the existing analysis results can be checked sequentially. This will allow us to consider new observation plans and to improve the efficiency and accuracy of the analysis of the subsurface structure model. In this study, a database of geotechnical information was constructed for the San-in region. The database of geotechnical information includes the results of microtremor and gravity survey, analysis of ground structure by seismic observation, and borehole data in Tottori and Shimane prefectures. In addition, we constructed a system to display the constructed database on a map in a web browser (Web-GIS). For the base system of the GIS, Leaflet, a Java Script library, was used to display the prepared database of geotechnical information using the GSI tiled map as the base map. The developed database and GIS system will be used to researchers and the public in the future.
Identification of enterotoxigenic Bacteroides fragilis in patients with diarrhea: a study targeting 16S rRNA, gyrB and nanH genes
Authors: Amiri Rosa , Norouzbabaei Zahra , Kalali Naeemeh , Ghourchian Sedighe , Yaseri Mehdi , Abdollahi Alireza , Douraghi Masoumeh 
Published: October 2021

Abstract
Background We aimed to identify the enterotoxigenic Bacteroides fragilis (ETBF) and bft subtypes among patients with diarrhea. In addition, we assessed whether DNA gyrase subunit B (gyrB) and neuraminidase (nanH) genes are useful determinants for identification of B. fragilis compared to 16S rDNA sequencing as a reference method. Results A total of 111 Gram-negative anaerobic coccobacilii were isolated from 530 fecal specimens using BBE agar. Of the 111 isolates, 100 (90.09%) were assumed to be a member of Bacteroides fragilis group as they yielded an amplicon through PCR using the group-specific primers (Bfra-F/g-Bfra-R). However, only 28 isolates out of 100 were definitively identified as species of Bacteroides using16S rRNA gene sequencing; of which 15 isolates were B. fragilis and the remaining 13 isolates were identified as B. thetaiotaomicron (n=6), Parabacteroides distasonis (n=3), B. vulgatus (Phocaeicola vulgatus) (n=1), B. ovatus (n=1), B. congonensis (n=1) and B. nordii (n=1). Among the 15 isolates of B. fragilis, 4 were found to be ETBF. Compared to the reference method, the specificity and accuracy of the PCR targeting gyrB gene (64.7% and 65%) was higher than of nanH (36.4% and 46%, respectively.ConclusionsThis study demonstrated that more than one-fourth of B. fragilis isolates harbored bft gene and less than 1% of patients with diarrhea harbored ETBF. The slight agreement between the PCR assays -already used for identification of B. fragilis which targeting gyrB or nanH - and 16S rRNA gene sequencing as the reference method was noted.