bluuebunny/crossref_metadata_2025_split huggingface dataset:
A T-cell independent universal cellular therapy strategy through antigen depletion
Authors: Li Dan , Wang Wenbing , Xie Shufeng , Ge Maolin , Wang Ruiheng , Xu Qiongyu , Sun Yan , Zhu Jiang , Liu Han 
Published: March 2021

AbstractCD19-targeting chimeric antigen receptor (CAR) T-cell therapeutics is a revolutionary, novel and successful treatment for B-cell malignancies. However, while CD19-CAR-T therapy can obtain high rates of complete responses in these patients, a significant fraction of patients may experience CD19-negative relapse. Moreover, the dependency on T-cell mediated cytotoxicity restricts CAR-T therapy as a patient-specific individualized therapy with severe side effects such as cytokine-release syndrome (CRS). Whether CAR-T therapy can be substituted by a non-T-cell based universal cellular therapy is largely unknown. Surprisingly, we have demonstrated here that T-lymphocytic cells, as well as non-lymphocytic cells, can cause CD19 internalization and subsequent depletion when they are armed with a CD19-recognizing moiety. This CD19 antigen depletion can efficiently induce T-cell independent apoptosis in target cancer cells whose survival is dependent upon CD19 expression, suggesting that CD19 antigen depletion constitutes a crucial tumor destroying mechanism for CD19-CAR-T, especially for its long-term efficacy. We therefore proposed a universal strategy for CRS-free cellular therapeutics, utilizing artificial antigen-recognizing cells (AARC), which can be manufactured universally and standardly as “off-the-shelf” mesenchymal stromal cells (MSCs) or other types of non-autologous cell expressing anergic CARs. Our results not only uncovered an unrecognized mechanism for CAR-T cytotoxicity and antigen loss, but also shed new insight into a shift in cellular therapeutics from unique patient-specific autologous therapeutics, to universal and standardized allogeneic treatment.
Reproducible Coactivation Patterns of Functional Brain Networks Reveal the Aberrant Dynamic State Transition in Schizophrenia
Authors: Yang Hang , Zhang Hong , Di Xin , Wang Shuai , Meng Chun , Tian Lin , Biswal Bharat 
Published: March 2021

AbstractIt is well documented that massive dynamic information is contained in the resting-state fMRI. Recent studies have identified recurring states dominated by similar coactivation patterns (CAP) and revealed their temporal dynamics. However, the reproducibility and generalizability of the CAP analysis is unclear. To address this question, the effects of methodological pipelines on CAP are comprehensively evaluated in this study, including preprocessing, network construction, cluster number and three independent cohorts. The CAP state dynamics are characterized by fraction of time, persistence, counts, and transition probability. Results demonstrate six reliable CAP states and their dynamic characteristics are also reproducible. The state transition probability is found to be positively associated with the spatial similarity. Furthermore, the aberrant CAP in schizophrenia has been investigated by using the reproducible method on three cohorts. Schizophrenia patients spend less time in CAP states that involve the fronto-parietal network, but more time in CAP states that involve the default mode and salience network. The aberrant dynamic characteristics of CAP are correlated with the symptom severity. These results reveal the reproducibility and generalizability of the CAP analysis, which can provide novel insights into the neuropathological mechanism associated with aberrant brain network dynamics of schizophrenia.HighlightsThree coactivation patterns (CAPs) pairs with opposite coactivation profiles were identified, and the between-state transition probability was positively correlated with their spatial similarity.Good spatial and temporal reproducibility and generalizability of CAPs were achieved under varied analytic methods and independent cohorts.Schizophrenia patients showed altered temporal dynamics not only within the triple-network but also other primary and higher-order networks.
NRF1 Association with AUTS2-Polycomb Mediates Specific Gene Activation in the Brain
Authors: Liu Sanxiong , Aldinger Kimberly A , Cheng Chi Vicky , Kiyama Takae , Dave Mitali , McNamara Hanna K. , Caraffi Stefano G , Ivanovski Ivan , Errichiello Edoardo , Zweier Christiane , Zuffardi Orsetta , Schneider Michael , Papavasiliou Antigone S. , Perry M. Scott , Cho Megan T , Weber Astrid , Swale Andrew , Badea Tudor C. , Mao Chai-An , Garavelli Livia , Dobyns William B , Reinberg Danny 
Published: March 2021

SUMMARYThe heterogeneous complexes comprising the family of Polycomb Repressive Complex 1 (PRC1) are instrumental to establishing facultative heterochromatin that is repressive to transcription. Yet, two PRC1 species, PRC1.3 and PRC1.5, are known to comprise novel components, AUTS2, P300, and CK2 that convert this repressive function to that of transcription activation. Here, we report that patients harboring mutations in the HX repeat domain of AUTS2 exhibit defects in AUTS2 and P300 interaction as well as a developmental disorder reflective of Rubinstein-Taybi syndrome, which is mostly associated with a heterozygous pathogenic variant inCREBBP/EP300. As well, the absence of AUTS2 gives rise to a mis-regulation of a subset of developmental genes and curtails motor neuron differentiation from embryonic stem cells in the context of a well-defined system. Moreover, the transcription factor, Nuclear Respiratory Factor 1 (NRF1) exhibits a novel and integral role in this aspect of the neurodevelopmental process, being required for PRC1.3 recruitment to chromatin.
Don’t Stress, It’s Under Control: Neural Correlates of Stressor Controllability in Humans
Authors: Meine Laura E. , Meier Jana , Meyer Benjamin , Wessa Michèle 
Published: March 2021

AbstractAnimal research has repeatedly shown that experience of control over an aversive event can protect against the negative consequences of later uncontrollable stress. Neurobiologically, this effect is assumed to correspond to persistent changes in the pathway linking the ventromedial prefrontal cortex (vmPFC) and the dorsal raphe nucleus. However, it remains unclear to what extent these findings translate to humans. During functional magnetic resonance imaging, we subjected participants to controllable and uncontrollable aversive but non-painful electric stimuli, as well as to a control condition without aversive stimulation. In each trial, a symbol signalled whether participants could terminate the stressor through correct performance in a button-matching task or whether the stressor would be randomly terminated, i.e., uncontrollable. Along with neural responses, we assessed participants’ accuracy, reaction times, and heart rate. To relate neural activations and subjective experience, we asked participants to rate perceived control, helplessness, and stress. Results were largely in line with our hypotheses. The vmPFC was generally deactivated by stress, but this effect was attenuated when participants could terminate the stressor compared to when their responses had no effect. Furthermore, activation in stress-responsive regions, including the bilateral insula, was reduced during controllable trials. Under uncontrollable stress, greater vmPFC recruitment was linked to reduced feelings of helplessness. An investigation of condition-dependent differences in vmPFC connectivity yielded no significant results. Our findings further corroborate animal research and emphasise the role of the vmPFC in controllability-dependent regulation of stress responses. Based on the results, we discuss future directions in the context of resilience research and mental health promotion.
A constitutively expressed fluorescence ubiquitin cell cycle indicator (FUCCI) in axolotls for studying tissue regeneration
Authors: Duerr Timothy J , Jeon Eun Kyung , Wells Kaylee M , Villanueva Antonio , Seifert Ashley W , McCusker Catherine D , Monaghan James R 
Published: March 2021

AbstractRegulation of cell cycle progression is essential for cell proliferation during regeneration following injury. After appendage amputation, the axolotl (Ambystoma mexicanum) regenerates missing structures through an accumulation of proliferating cells known as the blastema. To study cell division during blastema growth, we generated a transgenic line of axolotls that ubiquitously expresses a bicistronic version of the Fluorescent Ubiquitination-based Cell Cycle Indicator (FUCCI). We demonstrate near-ubiquitous expression of FUCCI expression in developing and adult tissues and validate these expression patterns with DNA synthesis and mitosis phase markers. We demonstrate the utility of FUCCI for live and whole-mount imaging, showing the predominantly local contribution of cells during limb and tail regeneration. We also show that spinal cord amputation results in increased proliferation at least 5 mm from the injury. Finally, we use multimodal staining to provide cell type information for cycling cells by combining fluorescencein-situhybridization, EdU click-chemistry, and immunohistochemistry on a single FUCCI tissue section. This new line of animals will be useful for studying cell cycle dynamics usingin-situendpoint assays andin-vivoimaging in developing and regenerating animals.Summary statementWe generated a ubiquitous transgenic fluorescence ubiquitin cell cycle indicator (FUCCI) axolotl line for examination of cell cycle dynamics during tissue regeneration.
Does effects of brand origin misperception jeopardize brand equity?
Authors: Tseng Ting-Hsiang , Chan Nga Cheng , Liu Matthew Tingchi , Lin Chieh-Yu 
Published: March 2021

PurposeThe purpose of this study is to examine the effects of brand origin (BO) misperception (hereafter BOM) or non-identification on brand equity. Besides, the current study investigates the moderating role of brand strength in the relationship between BOM and brand equity.Design/methodology/approachThe current study adopted a 4 (BO identification: favorable BOM vs adverse BOM vs non-identification vs correct identification) × 2 (brand strength: strong vs weak) between-subjects design. A total number of 547 participants performed assessments on the automotive brand. The current study selected three strong brands and three weak brands for tests. In the experiment, respondents had to associate the brand with its country of origin. The assignment of BO conditions was based upon respondents' natural responses provided. ANOVA was used for data analysis.FindingsThe results indicate that as compared to correct BO identification, BOM (either adverse or favorable) or non-identification exerts a more negative impact on brand equity. Moreover, the study demonstrates that brand strength moderates the effect of perceived BO on brand equity.Originality/valueThis study provides empirical support to the notion that BOM is detrimental to brand equity. Specifically, when adverse BOM occurs, a strong brand suffers more from the negative consequences resulted than a weak brand does. Conversely, when consumers misattribute the BO to a country with a stronger image than its real origin (i.e. favorable BOM), the resulting negative effect is reversed. Moreover, the non-identification of BO hurts the brand equity of both strong and weak brands.
A novel fractional discrete nonlinear grey Bernoulli model for forecasting the wind turbine capacity of China
Authors: Wu Wen-Ze , Xie Wanli , Liu Chong , Zhang Tao 
Published: March 2021

PurposeA new method for forecasting wind turbine capacity of China is proposed through grey modelling technique.Design/methodology/approachFirst of all, the concepts of discrete grey model are introduced into the NGBM(1,1) model to reduce the discretization error from the differential equation to its discrete forms. Then incorporating the conformable fractional accumulation into the discrete NGBM(1,1) model is carried out to further improve the predictive performance. Finally, in order to effectively seek the emerging coefficients, namely, fractional order and nonlinear coefficient, the whale optimization algorithm (WOA) is employed to determine the emerging coefficients.FindingsThe empirical results show that the newly proposed model has a better prediction performance compared to benchmark models; the wind turbine capacity from 2019 to 2021 is expected to reach 275954.42 Megawatts in 2021. According to the forecasts, policy suggestions are provided for policy-makers.Originality/valueBy combing the fractional accumulation and the concepts of discrete grey model, a new method to improve the prediction performance of the NGBM(1,1) model is proposed. The newly proposed model is firstly applied to predict wind turbine capacity of China.
Fading and transformation: how a woman entrepreneur's multiple identities interact throughout her life
Authors: Cesaroni Francesca Maria , Sentuti Annalisa , Pediconi Maria Gabriella 
Published: March 2021

PurposeThis paper aims to further the understanding of women entrepreneurs' multiple identities by exploring how they interact throughout women's life cycles.Design/methodology/approachThis article combines a case study and a narrative analysis to investigate the experience of a woman who founded a business and retired after passing it on to her son. Data were collected by combining two different methods: biographical interview and follow-up interviews or conversations.FindingsFindings show that interactions between a woman entrepreneur's multiple identities may evolve through two main processes of change: transformation and fading. In the transformation process, adverse interactions between identities turn into synergistic and fruitful relationships. In the fading process, conflicts between identities gradually disappear, giving way to peaceful coexistence. Women's agency proves paramount in making these processes possible and helping her achieve personal and professional fulfillment.Originality/valueIn prior studies, women entrepreneurs have mostly been observed at a specific time or stage in their life and entrepreneurial experience. This paper responds to the call for the adoption of a dynamic perspective in the analysis of interactions among a woman entrepreneur's multiple identities so as to show how they may evolve during her entrepreneurial experience.
Thymoquinone chemosensitizes human colorectal cancer cells to imatinib via uptake/efflux genes modulation
Authors: Thabet Nadia A. , El‐Khouly Dalia , Sayed‐Ahmed Mohamed M. , Omran Mervat M. 
Published: March 2021

AbstractImatinib (IM) is a pharmaceutical drug that inhibits tyrosine kinase enzymes that are responsible for the activation of many proteins by signal transduction cascades as c‐Abl, c‐Kit and the platelet‐derived growth factor (PDGF) receptor. Thymoquinone (TQ) is an active constituent of Nigella sativa seeds. Thymoquinone benefits are attributed to its medicinal uses as antioxidant, anticancer and antimicrobial agent. This study aimed to investigate the impact of using TQ with IM in the HCT116 human colorectal cancer cell line model. The HCT116 cells were treated with IM or/and TQ in non‐constant ratios, in which the fixed concentrations of TQ (5, 10 or 20 µmol/L) were co‐treated with various concentrations of IM (7.5–120 µmol/L) for 24, 48 and 72 hours. Imatinib‐TQ interaction was analysed using CompuSyn software. The IC50 values for IM were 105, 72 μmol/L after 48 and 72 hours, respectively, and were significantly reduced to 7.3, 7 and 5.5 μmol/L after combination with TQ (10 μmol/L) and to 5.8, 5.6 and 4.6 μmol/L after combination with TQ (20 μmol/L) to 24, 48 and 72 hours, respectively. The combination index (CI) and dose reduction index (DRI) values indicate a significant synergism in HCT‐116 cells at different treatment time points. Thymoquinone significantly enhances the cellular uptake of IM in HCT116 cells in a time and concentration‐dependent manner. A significant downregulation in ATP‐binding cassette (ABC) subfamily B member 1 (ABCB1), ABC subfamily G member 2 (ABCG2) and human organic cation transporter 1 (hOCT1) genes was observed in the cells exposed to IM+TQ combination as compared to IM alone, which resulted in a substantial elevation in uptake/efflux ratio in combination group. In conclusion, TQ potentiates IM efficacy on HCT116 cells via uptake/efflux genes modulation.
Media framing of fighting COVID‐19 in China
Authors: Gui Lili 
Published: March 2021

AbstractThis study focuses on media framings of COVID‐19 in Chinese social and cultural contexts. After analysing nine weeks’ news reports of Xinwen Lianbo, one of China's mainstream media, we discovered that the metaphorical war frame dominated throughout the corpus but it did not remain the same. Some of its semantic concepts like the type of war evolved over time. There are also several minor metaphors such as race, challenge, chess and combination blow metaphors, which are not as dominant as the war metaphor but should by no means be neglected. The race and challenge metaphors are complementary with the war metaphor to frame COVID‐19, while the chess and combination blow metaphors are culturally loaded. What's more, literal frames like responsibility and collaboration were also found in the corpus. All frames, whether metaphorical or literal, work together to shape what COVID‐19 was, what roles people should play and what people could do. In this study, we intend to explore how media framings of COVID‐19 were related to media and government's responses as well as how they might have contributed to the public's responses. The media is an important source of information, whose role in public health emergencies deserves our attention.
Less Information, More Comparison, and Better Performance: Evidence from a Field Experiment
Authors: EYRING HENRY , FERGUSON PATRICK J. , KOPPERS SEBASTIAN 
Published: March 2021

ABSTRACTWe use a field experiment in professional sports to compare effects of providing absolute, relative, or both absolute and relative measures in performance reports for employees. Although studies have documented that the provision of these types of measures can benefit performance, theory from economic and accounting literature suggests that it may be optimal for firms to direct employees’ attention to some types of measures by omitting others. In line with this theory, we find that relative performance information alone yields the best performance effects in our setting—that is, that a subset of information (relative performance information) dominates the full information set (absolute and relative performance information together) in boosting performance. In cross‐sectional and survey‐data analyses, we do not find that restricting the number of measures shown per se benefits performance. Rather, we find that restricting the type of measures shown to convey only relative information increases involvement in peer‐performance comparison, benefitting performance. Our findings extend research on weighting of and responses to measures in performance reports.
A proctolin‐like peptide is regulated after baculovirus infection and mediates in caterpillar locomotion and digestion
Authors: Llopis‐Giménez Angel , Parenti Stefano , Han Yue , Ros Vera I. D. , Herrero Salvador 
Published: March 2021

AbstractBaculoviruses constitute a large group of invertebrate DNA viruses, predominantly infecting larvae of the insect order Lepidoptera. During a baculovirus infection, the virus spreads throughout the insect body producing a systemic infection in multiple larval tissues, included the central nervous system (CNS). As a main component of the CNS, neuropeptides are small protein‐like molecules functioning as neurohormones, neurotransmitters, or neuromodulators. These peptides are involved in regulating animal physiology and behavior and could be altered after baculovirus infection. In this study, we have investigated the effect of Spodoptera exigua multiple nucleopolyhedrovirus (SeMNPV) infection on expression of Spodoptera exigua neuropeptides and neuropeptide‐like genes. Expression of the gene encoding a polypeptide that resembles the well‐known insect neuropeptide proctolin and named as proctolin‐like peptide (PLP), was downregulated in the larval brain following infection and was chosen for further analysis. A recombinant Autographa californica multiple nucleopolyhedrovirus (AcMNPV) overexpressing the C‐terminal part of the PLP was generated and used in bioassays using S. exigua larvae to study its influence on the viral infection and insect behavior. AcMNPV‐PLP‐infected larvae showed less locomotion activity and a reduction in growth compared to larvae infected with wild type AcMNPV or mock‐infected larvae. These results are indicative of this new peptide as a neuromodulator that regulates visceral and skeletal muscle contractions and offers a novel effector involved in the behavioral changes during baculovirus infection.
Fluid‐driven Hydrovolcanic Activity along Fracture Zones and near Seamounts: Evidence from Deep‐sea Fe‐rich Spherules, Central Indian Ocean Basin
Authors: AMONKAR Ankeeta , IYER Sridhar D. , BABU Elapavalooru V.S.S.K. , SHAILAJHA Natarajan , SARDAR Areef , MANJU S. 
Published: March 2021

AbstractAn insight on occurrence of Fe‐rich spherules from the Central Indian Ocean Basin (CIOB) provides an understanding of their distribution at a water depth of >5,000 m. In the present study, Fe‐rich spherules are identified to occur in two different sediment types (i.e., siliceous and pelagic) and tectonic settings (i.e. near seamounts and fracture zones). These are single spheres or aggregates, of different sizes (63 to 390 μm) and show textural variability (smooth/quenched, brickwork, corkscrew, interlocking and dendritic). A comparative study based on physical morphology and chemical composition suggests a common mechanism of formation. The association of spherules with fracture zones (FZ) and seamounts signifies that morpho‐tectonic features play an important role in fluid‐driven hydrovolcanism. Based on the evidence and geologic conditions existing in the basin, we conclude that molten fuel‐coolant interaction (MFCI) coupled with submarine hydrothermal exhalations could be an ideal mechanism for the formation of spherules and Fe‐particles. The accretion of the spherules on the surface sediments could be a result of recent volcanic phenomena, while those occurring at different depths (280–355, and 460–475 cm‐bsf) within the sediment core indicate two different episodes. The study provides a global implication in understanding fluid‐driven magmatism in a deep‐sea intraplate environment.
Geochemistry and Geochronology of the Jinghong Ophiolites: Implications for the Tectonic Evolution of the Eastern Paleo‐Tethys
Authors: ZHU Jianjiang , LIU Fulai , WANG Fang , XIE Shiwen , CAI Jia , JI Lei , WANG Huining 
Published: March 2021

AbstractThe Jinghong mafic–ultramafic complex, exposed in the eastern margin of the Lancangjiang tectonic belt, is related to the subduction of the Paleo‐Tethys Ocean. Its petrogenesis plays a key role in constraining the tectonic evolution of the eastern Paleo‐Tethys Ocean in southwestern China. In this study, we present petrological, geochemical and geochronological results of the Jinghong complex rocks, in order to decipher their origin and tectonic significance. The Jinghong mafic–ultramafic complex was composed of peridotite, gabbro, basalt and minor plagiogranite. Whole‐rock geochemical data of the mafic rocks indicate that they have both MORB and IAB affinities and plot in the back‐arc basin basalt (BABB) field in the FeO∗/MgO vs. TiO2 diagram. Combined with their trace element characteristics, it can be concluded that the Jinghong mafic–ultramafic complex represents an ophiolite suite that was formed in a back‐arc ocean basin. Precise LA‐ICP‐MS zircon U‐Pb dating yielded weighted mean 206Pb/238U ages of 298.4 ± 1.7 Ma, 294.3 ± 1.6 Ma, and 292.8 ± 2.0 Ma for gabbroic rocks from this complex, which indicates that the Jinghong ophiolites were formed during the early Permian (298–293 Ma). We propose that during subduction of the main Paleo‐Tethys Ocean, a back‐arc ocean basin was formed at the east of the Lancangjiang tectonic belt.