bluuebunny/crossref_metadata_2025_split huggingface dataset:
Determinants of outcomes following surgery for type A acute aortic dissection: the UK National Adult Cardiac Surgical Audit
Authors: Benedetto Umberto , Dimagli Arnaldo , Kaura Amit , Sinha Shubhra , Mariscalco Giovanni , Krasopoulos George , Moorjani Narain , Field Mark , Uday Trivedi , Kendal Simon , Cooper Graham , Uppal Rakesh , Bilal Haris , Mascaro Jorge , Goodwin Andrew , Angelini Gianni , Tsang Geoffry , Akowuah Enoch 
Published: August 2021

Abstract

Aims
Operability of type A acute aortic dissections (TAAAD) is currently based on non-standardized decision-making process, and it lacks a disease-specific risk evaluation model that can predict mortality. We investigated patient, intraoperative data, surgeon, and centre-related variables for patients who underwent TAAAD in the UK.

Methods and results
We identified 4203 patients undergoing TAAAD surgery in the UK (2009–18), who were enrolled into the UK National Adult Cardiac Surgical Audit dataset. The primary outcome was operative mortality. A multivariable logistic regression analysis was performed with fast backward elimination of variables and the bootstrap-based optimism-correction was adopted to assess model performance. Variation related to hospital or surgeon effects were quantified by a generalized mixed linear model and risk-adjusted funnel plots by displaying the individual standardized mortality ratio against expected deaths. Final variables retained in the model were: age [odds ratio (OR) 1.02, 95% confidence interval (CI) 1.02–1.03; P &lt; 0.001]; malperfusion (OR 1.79, 95% CI 1.51–2.12; P &lt; 0.001); left ventricular ejection fraction (moderate: OR 1.40, 95% CI 1.14–1.71; P = 0.001; poor: OR 2.83, 95% CI 1.90–4.21; P &lt; 0.001); previous cardiac surgery (OR 2.29, 95% CI 1.71–3.07; P &lt; 0.001); preoperative mechanical ventilation (OR 2.76, 95% CI 2.00–3.80; P &lt; 0.001); preoperative resuscitation (OR 3.36, 95% CI 1.14–9.87; P = 0.028); and concomitant coronary artery bypass grafting (OR 2.29, 95% CI 1.86–2.83; P &lt; 0.001). We found a significant inverse relationship between surgeons but not centre annual volume with outcomes.

Conclusions
Patient characteristics, intraoperative factors, cardiac centre, and high-volume surgeons are strong determinants of outcomes following TAAAD surgery. These findings may help refining clinical decision-making, supporting patient counselling and be used by policy makers for quality assurance and service provision improvement.
Interaction of sex, age, body mass index and race on hypertension risk in the American population: a cross-sectional study
Authors: Zhang Zhenhong , Zhang Tao , Zhao Enhui , Ding Shihan , Kang Xiao , Zhang Wenwen , Liu Bingkun , Liu Haoran , Cheng Anlan , Li Guoju , Wang Qiuzhen 
Published: August 2021

Abstract

Background
The joint effects of sex, age, body mass index (BMI) and race on hypertension have not been fully addressed. Herein, we carried out this study aiming to investigate the possible effects of the interaction of sex, age, BMI and race on risk of hypertension.

Methods
By using the data of a sample-adjusted 2656 women and 2515 men in American National Health and Nutrition Examination Survey 2015–16, we analyzed the interaction of sex, age, BMI and race by logistic regression models, followed by strata-specific analyses. Hypertension was defined as a systolic blood pressure ≥130 mmHg/diastolic blood pressure ≥80 mmHg or taking anti-hypertensive medication.

Results
A total of 5171 participants were included in analysis, and the prevalence of hypertension was 53.68%. The interactive effect of sex and age, BMI and age, race and age were statistically significant on hypertension. Strata-specific analyses showed that female at 40 years and above were positively associated with hypertension than those at 20–39 years. The associations also persistence in male. The risk estimates for age ≥40 on hypertension were consistently positive across all overweight/obesity and race groups. The effect was most prominent among overweight populations aged 60–80 years and Other Hispanic aged 40 years and above.

Conclusion
There exists interactive effect of sex and age, BMI and age, race and age on hypertension in American population. The effect of age on hypertension was more prominent in female, overweight populations and Other Hispanic populations. Differences in age, BMI and race should be considered when providing corresponding antihypertensive measures.
Public perceptions and behavioural responses to the first COVID-19 pandemic wave in Italy: results from the iCARE study
Authors: Stojanovic Jovana , Raparelli Valeria , Boccia Stefania , Bacon Simon L , Lavoie Kim L ,   for the iCARE Study Team
Published: August 2021

Abstract

Background
Italy was the first European country to be affected by COVID-19. Considering that many countries are currently battling the second wave of the pandemic, understanding people’s perceptions and responses to government policies remain critical for informing on-going mitigation strategies. We assessed attitudes towards COVID-19 policies, levels of adherence to preventive behaviours, and the association between COVID-19 related concerns and adherence levels.

Methods
We recruited a convenience sample of Italian individuals from an international cross-sectional survey (www.icarestudy.com) from 27 March to 5 May 2020. Multivariate regression models were used to test the association between concerns and the adoption of preventive measures.

Results
The survey included 1332 participants [female (68%), younger than 25 (57%)] that reported high awareness (over 96%) and perceived importance (88%) of policies. We observed varied levels of adherence to: hand-washing (96%), avoiding social gatherings (96%), self-isolation if suspected or COVID-19 positive (77%). Significantly lower adherence to self-isolation was reported by individuals with current employment. High levels of concerns regarding health of other individuals and country economy were reported. Only health concerns for others were significantly associated with higher adherence to hand-washing behaviour.

Conclusions
In order to inform current/future government strategies, we provide insights about population’s responses to the initial pandemic phase in Italy. Communication approaches should consider addressing people’s concerns regarding the health of other individuals to motivate adherence to prevention measures. Provision of social and economic support is warranted to avoid unequal impacts of governmental policies and allow effective adherence to self-isolating measures.
Invasion of the choroid plexus epithelium byNeisseria meningitidisis differently mediated by Arp2/3 signaling and possibly by dynamin dependent on the presence of the capsule
Authors: Herold Rosanna , Sünwoldt Gina , Stump-Guthier Carolin , Weiss Christel , Ishikawa Hiroshi , Schroten Horst , Adam Rüdiger , Schwerk Christian 
Published: August 2021

ABSTRACTNeisseria meningitis (Nm) is a human-specific bacterial pathogen that can cause sepsis and meningitis. To cause meningitis Nm must enter the central nervous system (CNS) across one of the barriers between the blood and the brain. We have previously shown that a capsule-depleted Serogroup B strain of Nm displays enhanced invasion into human choroid plexus (CP) epithelial papilloma (HIBCPP) cells, which represent an in vitro model of the blood–cerebrospinal fluid barrier (BCSFB). Still, the processes involved during CNS invasion by Nm, especially the role of host cell actin cytoskeleton remodeling, are not investigated in detail. Here, we demonstrate that invasion into CP epithelial cells by encapsulated and capsule-depleted Nm is mediated by distinct host cell pathways. Whereas a Serogroup B wild-type strain enters HIBCPP cells by a possibly dynamin-independent, but actin related protein 2/3 (Arp2/3)-dependent mechanism, invasion by a capsule-depleted mutant is reduced by the dynamin inhibitor dynasore and Arp2/3-independent. Both wild-type and mutant bacteria require Src kinase activity for entry into HIBCPP cells. Our data show that Nm can employ different mechanisms for invasion into the CP epithelium dependent on the presence of a capsule.
The ins and outs of Bacillus proteases: activities, functions and commercial significance
Authors: Harwood Colin R , Kikuchi Yoshimi 
Published: August 2021

ABSTRACT
Because the majority of bacterial species divide by binary fission, and do not have distinguishable somatic and germline cells, they could be considered to be immortal. However, bacteria ‘age’ due to damage to vital cell components such as DNA and proteins. DNA damage can often be repaired using efficient DNA repair mechanisms. However, many proteins have a functional ‘shelf life’; some are short lived, while others are relatively stable. Specific degradation processes are built into the life span of proteins whose activities are required to fulfil a specific function during a prescribed period of time (e.g. cell cycle, differentiation process, stress response). In addition, proteins that are irreparably damaged or that have come to the end of their functional life span need to be removed by quality control proteases. Other proteases are involved in performing a variety of specific functions that can be broadly divided into three categories: processing, regulation and feeding. This review presents a systematic account of the proteases of Bacillus subtilis and their activities. It reviews the proteases found in, or associated with, the cytoplasm, the cell membrane, the cell wall and the external milieu. Where known, the impacts of the deletion of particular proteases are discussed, particularly in relation to industrial applications.
The Molecular Basis of Glucose Galactose Malabsorption in a Large Swedish Pedigree
Authors: Lostao M Pilar , Loo Donald D , Hernell Olle , Meeuwisse Gunnar , Martin Martin G , Wright Ernest M 
Published: August 2021

Abstract
Glucose-galactose malabsorption (GGM) is due to mutations in the gene coding for the intestinal sodium glucose cotransporter SGLT1 (SLC5A1). Here we identify the rare variant Gln457Arg (Q457R) in a large pedigree of patients in the Västerbotten County in Northern Sweden with the clinical phenotype of GGM. The functional effect of the Q457R mutation was determined in protein expressed in Xenopus laevis oocytes using biophysical and biochemical methods. The mutant failed to transport the specific SGLT1 sugar analog α-methyl-D-glucopyranoside (αMDG). Q457R SGLT1 was synthesized in amounts comparable to the wild-type (WT) transporter. SGLT1 charge measurements and freeze-fracture electron microscopy demonstrated that the mutant protein was inserted into the plasma membrane. Electrophysiological experiments, both steady-state and presteady-state, demonstrated that the mutant bound sugar with an affinity lower than the WT transporter. Together with our previous studies on Q457C and Q457E mutants, we established that the positive charge on Q457R prevented the translocation of sugar from the outward-facing to inward-facing conformation. This is contrary to other GGM cases where missense mutations caused defects in trafficking SGLT1 to the plasma membrane. Thirteen GGM patients are now added to the pedigree traced back to the late 17th century. The frequency of the Q457R variant in Västerbotten County genomes, 0.0067, is higher than in the general Swedish population, 0.0015, and higher than the general European population, 0.000067. This explains the high number of GGM cases in this region of Sweden.
Analysis of blood group antigens on MUC5AC in mucinous ovarian cancer tissues using in situ proximity ligation assay
Authors: Mateoiu Constantina , Vitiazeva Varvara , Kristjansdottir Björg , Weijdegård Birgitta , Örnros Jessica , Gallini Radiosa , Kamali-Moghaddam Masood , Sundfeldt Karin , Karlsson Niclas G 
Published: August 2021

Abstract
MUC5AC has been indicated to be a marker for mucinous ovarian cancer (OC). We investigated the use of in situ proximity ligation assay (PLA) for blood group ABH expressing MUC5AC to differentiate between serous and mucinous OC, to validate preceding observations that also MUC5AC ABH expression is increased in mucinous OC. We developed PLA for anti-A, B, and H/anti-MUC5AC and a PLA using a combined lectin Ulex europaeus agglutinin I (UEA I)/anti-MUC5AC assay. The PLAs were verified with mass spectrometry, where mucinous OC secretor positive patients’ cysts fluids containing ABH O-linked oligosaccharides also showed positive OC tissue PLA staining. A nonsecretor mucinous OC cyst fluid was negative for ABH and displayed negative PLA staining of the matched tissue. Using the UEA I/MUC5AC PLA, we screened a tissue micro array of 410 ovarian tissue samples from patients with various stages of mucinous or serous OC, 32 samples with metastasis to the ovaries and 34 controls. The PLA allowed differentiating mucinous tumors with a sensitivity of 84% and a specificity of 97% both against serous cancer but also compared to tissues from controls. This sensitivity is close to the expected incidence of secretor individuals in a population. The recorded sensitivity was also found to be higher compared to mucinous type cancer with metastasis to the ovaries, where only 32% were positive. We conclude that UEA 1/MUC5AC PLA allows glycospecific differentiation between serous and mucinous OC in patients with positive secretor status and will not identify secretor negative individuals with mucinous OC.
Curvature effects and radial homoclinic snaking
Authors: Gomila Damià , Knobloch Edgar 
Published: August 2021

Abstract
In this work, we revisit some general results on the dynamics of circular fronts between homogeneous states and the formation of localized structures in two dimensions (2D). We show how the bifurcation diagram of axisymmetric structures localized in radius fits within the framework of collapsed homoclinic snaking. In 2D, owing to curvature effects, the collapse of the snaking structure follows a different scaling that is determined by the so-called nucleation radius. Moreover, in the case of fronts between two symmetry-related states, the precise point in parameter space to which radial snaking collapses is not a ‘Maxwell’ point but is determined by the curvature-driven dynamics only. In this case, the snaking collapses to a ‘zero surface tension’ point. Near this point, the breaking of symmetry between the homogeneous states tilts the snaking diagram. A different scaling law is found for the collapse of the snaking curve in each case. Curvature effects on axisymmetric localized states with internal structure are also discussed, as are cellular structures separated from a homogeneous state by a circular front. While some of these results are well understood in terms of curvature-driven dynamics and front interactions, a proper mathematical description in terms of homoclinic trajectories in a radial spatial dynamics description is lacking.
The clinical correlates of vaccine-induced immune thrombotic thrombocytopenia after immunisation with adenovirus vector-based SARS-CoV-2 vaccines
Authors: Gaunt Eleanor R , Mabbott Neil A 
Published: August 2021

Summary
We are at a critical stage in the COVID-19 pandemic where vaccinations are being rolled out globally, in a race against time to get ahead of the SARS-CoV-2 coronavirus and the emergence of more highly transmissible variants. A range of vaccines have been created and received either emergency approval or full licensure. To attain the upper hand, maximum vaccine synthesis, deployment, and uptake as rapidly as possible is essential. However, vaccine uptake, particularly in younger adults is dropping, at least in part fuelled by reports of rare complications associated with specific vaccines. This review considers how vaccination with adenovirus vector-based vaccines against the SARS-CoV-2 coronavirus might cause rare cases of thrombosis and thrombocytopenia in some recipients. A thorough understanding of the underlying cellular and molecular mechanisms that mediate this syndrome may help to identify methods to prevent these very rare, but serious side effects. This will also help facilitate the identification of those at highest risk from these outcomes, so that we can work towards a stratified approach to vaccine deployment to mitigate these risks.
Kinetics of Archived M184V Mutation in Treatment-Experienced Virally Suppressed HIV-Infected Patients
Authors: Palich Romain , Teyssou Elisa , Sayon Sophie , Abdi Basma , Soulie Cathia , Cuzin Lise , Tubiana Roland , Valantin Marc-Antoine , Schneider Luminita , Seang Sophie , Wirden Marc , Pourcher Valérie , Katlama Christine , Calvez Vincent , Marcelin Anne-Geneviève 
Published: August 2021

AbstractBackgroundWe aimed to assess the kinetics of drug-resistant viral variants (DRVs) harboring the M184V mutation in proviral DNA of long-term virally suppressed patients, and factors associated with DRV persistence.MethodsHuman immunodeficiency virus (HIV) DNA from blood cells stored in 2016 and 2019 was sequenced using Sanger and ultradeep sequencing (SS and UDS; detection threshold 1%) in antiretroviral therapy (ART)-treated patients with HIV RNA &lt; 50 copies/mL for at least 5 years, with past M184V mutation documented in HIV RNA.ResultsAmong 79 patients, by combining SS and UDS, M184V was found to be absent in 26/79 (33%) patients and persistent in 53/79 (67%). M184V-positive patients had a longer history of ART, lower CD4 nadir, and higher pretherapeutic HIV RNA. Among 37 patients with viral sequences assessed by UDS, the proportion of M184V-positive DRVs significantly decreased between 2016 and 2019 (40% vs 14%, P = .005). The persistence of M184V was associated with duration and level of HIV RNA replication under lamivudine/emtricitabine (3TC/FTC; P = .0009 and P = .009, respectively).ConclusionsWhile it decreased over time in HIV DNA, M184V mutation was more frequently persistent in HIV DNA of more treatment-experienced patients with longer past replication under 3TC/FTC.
Early antiretroviral therapy initiation effect on metabolic profile in vertically HIV-1-infected children
Authors: Tarancón-Diez Laura , Rull Anna , Herrero Pol , Vazquez-Alejo Elena , Peraire Joaquim , Guillén Sara , Navarro-Gomez Maria Luisa , Viladés Consuelo , Muñoz-Fernandez Mª Ángeles , Vidal Francesc 
Published: August 2021

Abstract

Background
Early combined antiretroviral treatment (cART) in perinatally acquired HIV-1 children has been associated with a rapid viral suppression, small HIV-1 reservoir size and reduced mortality and morbidity. Immunometabolism has emerged as an important field in HIV-1 infection offering both relevant knowledge regarding immunopathogenesis and potential targets for therapies against HIV-1.

Objectives
To characterize the proteomic, lipidomic and metabolomic profile of HIV-1-infected children depending on their age at cART initiation.

Patients and methods
Plasma samples from perinatally HIV-1-infected children under suppressive cART who initiated an early cART (first 12 weeks after birth, EARLY, n = 10) and late cART (12–50 weeks after birth, LATE, n = 10) were analysed. Comparative plasma proteomics, lipidomics and metabolomics analyses were performed by nanoLC-Orbitrap, UHPLC-qTOF and GC-qTOF, respectively.

Results
Seven of the 188 proteins identified exhibited differences comparing EARLY and LATE groups of HIV-1-infected children. Despite no differences in the lipidomic (n = 115) and metabolomic (n = 81) profiles, strong correlations were found between proteins and lipid levels as well as metabolites, including glucidic components and amino acids, with clinical parameters. The ratio among different proteins showed high discriminatory power of EARLY and LATE groups.

Conclusions
Protein signature show a different proinflammatory state associated with a late cART introduction. Its associations with lipid levels and the relationships found between metabolites and clinical parameters may potentially trigger premature non-AIDS events in this HIV-1 population, including atherosclerotic diseases and metabolic disorders. Antiretroviral treatment should be started as soon as possible in perinatally acquired HIV-1-infected children to prevent them from future long-life complications.
When to change treatment of acute invasive aspergillosis: an expert viewpoint
Authors: Slavin Monica A , Chen Yee-Chun , Cordonnier Catherine , Cornely Oliver A , Cuenca-Estrella Manuel , Donnelly J Peter , Groll Andreas H , Lortholary Olivier , Marty Francisco M , Nucci Marcio , Rex John H , Rijnders Bart J A , Thompson George R , Verweij Paul E , White P Lewis , Hargreaves Ruth , Harvey Emma , Maertens Johan A 
Published: August 2021

AbstractInvasive aspergillosis (IA) is an acute infection affecting patients who are immunocompromised, as a result of receiving chemotherapy for malignancy, or immunosuppressant agents for transplantation or autoimmune disease. Whilst criteria exist to define the probability of infection for clinical trials, there is little evidence in the literature or clinical guidelines on when to change antifungal treatment in patients who are receiving prophylaxis or treatment for IA. To try and address this significant gap, an advisory board of experts was convened to develop criteria for the management of IA for use in designing clinical trials, which could also be used in clinical practice. For primary treatment failure, a change in antifungal therapy should be made: (i) when mycological susceptibility testing identifies an organism from a confirmed site of infection, which is resistant to the antifungal given for primary therapy, or a resistance mutation is identified by molecular testing; (ii) at, or after, 8 days of primary antifungal treatment if there is increasing serum galactomannan, or galactomannan positivity in serum, or bronchoalveolar lavage fluid when the antigen was previously undetectable, or there is sudden clinical deterioration, or a new clearly distinct site of infection is detected; and (iii) at, or after, 15 days of primary antifungal treatment if the patient is clinically stable but with ≥2 serum galactomannan measurements persistently elevated compared with baseline or increasing, or if the original lesions on CT or other imaging, show progression by &gt;25% in size in the context of no apparent change in immune status.