bluuebunny/crossref_metadata_2025_split huggingface dataset:
Visual assessment of movement quality: a study on intra- and interrater reliability of a multi-segmental single leg squat test
Authors: Ressman John , Grooten Wilhelmus Johannes Andreas , Rasmussen-Barr Eva 
Published: June 2021

Abstract
Background
The Single Leg Squat test (SLS) is a common tool used in clinical examination to set and evaluate rehabilitation goals, but there is not one established SLS test used in the clinic. Based on previous scientific findings on the reliability of the SLS test and with a methodological rigorous setup, the aim of the present study was to investigate the intra- and interrater reliability of a standardised multi-segmental SLS test.

Methods
We performed a study of measurement properties to investigate the intra- and interrater reliability of a standardised multi-segmental SLS test including the assessment of the foot, knee, pelvis, and trunk. Novice and experienced physiotherapists rated 65 video recorded SLS tests from 34 test persons. We followed the Quality Appraisal for Reliability Studies checklist.

Results
Regardless of the raters experience, the interrater reliability varied between “moderate” for the knee variable (ĸ = 0.41, 95% CI 0.10–0.72) and “almost perfect” for the foot (ĸ = 1.00, 95% CI 1.00–1.00). The intrarater reliability varied between “slight” (pelvic variable; ĸ = 0.17, 95% CI -0.22-0.55) to “almost perfect” (foot variable; ĸ = 1.00, 95% CI 1.00–1.00; trunk variable; ĸ = 0.82, 95% CI 0.66–0.97). A generalised kappa coefficient including the values from all raters and segments reached “moderate” interrater reliability (ĸ = 0.52, 95% CI 0.43–0.61), the corresponding value for the intrarater reliability reached “almost perfect” (ĸ = 0.82, 95% CI 0.77–0.86).

Conclusions
The present study shows a “moderate” interrater reliability and an “almost perfect” intrarater reliability for the variable all segments regardless of the raters experience. Thus, we conclude that the proposed standardised multi-segmental SLS test is reliable enough to be used in an active population.
The impacts of land-use and land-cover change on wetland ecosystem service values in peri-urban and urban area of Bahir Dar City, Upper Blue Nile Basin, Northwestern Ethiopia
Authors: Assefa Workiyie Worie , Eneyew Belachew Getnet , Wondie Ayalew 
Published: June 2021

Abstract
Background
Even though wetlands are essential in enhancing water quality and providing recreation and entertainment opportunities in urban areas, their values are overlooked by the decision-makers. Underestimation of the economic value of wetland ecosystem services contributes to their continuing deterioration and inevitable loss. Investigating the changes in ecosystem service values (ESV) can provide crucial information for decision-making. This study, therefore, analyzes the temporal and spatial land-use/land-cover (LULC) change patterns over 35 years (1984–2019) intending to evaluate its impact on wetland ecosystem service values in Bahir Dar City, Ethiopia. Estimation and change analyses of ESVs were conducted by employing ArcGIS using LULC inputs of the year 1984, 1994, 2004, 2014, and 2019 with their corresponding global value coefficients that were developed earlier and our own modified value coefficients for the studied landscape.

Results
The results showed that wetlands and water bodies of the city and its peripheries had decreased by 75.71% (−1618 ha) within 35 years, while built-up area increased by 216.24% (+2599 ha). Cultivated land had increased slightly from 1984 to 1994 and then gradually declined since 1994. Changes in LULC had resulted in a decline of the total ESV. The total ESV had decreased from USD 29.73 × 106 to USD 20.84 × 106 in 35 years. This indicates the loss of nearly USD 8.9 × 106 ESV from 1984 to 2019. A sensitivity analysis suggested the robustness of ESV estimation in the study area. All individual ecosystem services experienced a negative change. However, a greater reduction in ESV was observed for services such as water regulation, waste treatment, and habitats for maintaining biodiversity. The expansion of built-up area of Bahir Dar City was the major factor that contributed to the loss of ESV provided by wetlands.

Conclusions
The loss of ESV resulting in LULC changes has a negative implication on local climate, waste management, and the livelihoods of the poor community. Thus, interventions should be made for the restoration and sustainable management of wetlands in the urban and peri-urban areas of Bahir Dar City.
Is the use of high correlated color temperature light at night related to delay of sleep timing in university students? A cross-country study in Japan and China
Authors: Higuchi Shigekazu , Lin Yandan , Qiu Jingjing , Zhang Yichi , Ohashi Michihiro , Lee Sang-il , Kitamura Shingo , Yasukouchi Akira 
Published: June 2021

AbstractBackgroundBlue-enriched white light at night has the potential to delay the circadian rhythm in daily life. This study was conducted to determine whether the use of high correlated color temperature (CCT) light at home at night is associated with delay of sleep timing in university students.MethodsThe survey was conducted in 2014–2015 in 447 university students in Japan and 327 students in China. Habitual sleep timing and type of CCT light at home were investigated by using a self-administered questionnaire. The Japanese students were significantly later than the Chinese students in bedtime, wake time, and midpoint of sleep. They were asked whether the lighting in the room where they spend most of their time at night was closer to warm color (low CCT) or daylight color (high CCT). The amount of light exposure level during daily life was measured for at least 1 week by the use of a light sensor in 60 students in each country.ResultsThe percentages of participants who used high CCT lighting at night were 61.6% for Japanese students and 80.8% for Chinese students. Bedtime and sleep onset time on school days and free days were significantly later in the high CCT group than in the low CCT group in Japan. The midpoint of sleep in the high CCT group was significantly later than that in the low CCT group on free days but not on school days. On the other hand, none of the sleep measurements on school days and free days were significantly different between the high CCT and low CCT groups in China. Illuminance level of light exposure during the night was significantly higher in Japanese than in Chinese, but that in the morning was significantly higher in China than in Japan.ConclusionsThe use of high CCT light at night is associated with delay of sleep timing in Japanese university students but not in Chinese university students. The effects of light at night on sleep timing and circadian rhythm may be complicated by other lifestyle factors depending on the country.
SARS-CoV-2 detection status associates with bacterial community composition in patients and the hospital environment
Authors: Marotz Clarisse , Belda-Ferre Pedro , Ali Farhana , Das Promi , Huang Shi , Cantrell Kalen , Jiang Lingjing , Martino Cameron , Diner Rachel E. , Rahman Gibraan , McDonald Daniel , Armstrong George , Kodera Sho , Donato Sonya , Ecklu-Mensah Gertrude , Gottel Neil , Salas Garcia Mariana C. , Chiang Leslie Y. , Salido Rodolfo A. , Shaffer Justin P. , Bryant Mac Kenzie , Sanders Karenina , Humphrey Greg , Ackermann Gail , Haiminen Niina , Beck Kristen L. , Kim Ho-Cheol , Carrieri Anna Paola , Parida Laxmi , Vázquez-Baeza Yoshiki , Torriani Francesca J. , Knight Rob , Gilbert Jack , Sweeney Daniel A. , Allard Sarah M. 
Published: June 2021

Abstract
Background
SARS-CoV-2 is an RNA virus responsible for the coronavirus disease 2019 (COVID-19) pandemic. Viruses exist in complex microbial environments, and recent studies have revealed both synergistic and antagonistic effects of specific bacterial taxa on viral prevalence and infectivity. We set out to test whether specific bacterial communities predict SARS-CoV-2 occurrence in a hospital setting.

Methods
We collected 972 samples from hospitalized patients with COVID-19, their health care providers, and hospital surfaces before, during, and after admission. We screened for SARS-CoV-2 using RT-qPCR, characterized microbial communities using 16S rRNA gene amplicon sequencing, and used these bacterial profiles to classify SARS-CoV-2 RNA detection with a random forest model.

Results
Sixteen percent of surfaces from COVID-19 patient rooms had detectable SARS-CoV-2 RNA, although infectivity was not assessed. The highest prevalence was in floor samples next to patient beds (39%) and directly outside their rooms (29%). Although bed rail samples more closely resembled the patient microbiome compared to floor samples, SARS-CoV-2 RNA was detected less often in bed rail samples (11%). SARS-CoV-2 positive samples had higher bacterial phylogenetic diversity in both human and surface samples and higher biomass in floor samples. 16S microbial community profiles enabled high classifier accuracy for SARS-CoV-2 status in not only nares, but also forehead, stool, and floor samples. Across these distinct microbial profiles, a single amplicon sequence variant from the genus Rothia strongly predicted SARS-CoV-2 presence across sample types, with greater prevalence in positive surface and human samples, even when compared to samples from patients in other intensive care units prior to the COVID-19 pandemic.

Conclusions
These results contextualize the vast diversity of microbial niches where SARS-CoV-2 RNA is detected and identify specific bacterial taxa that associate with the viral RNA prevalence both in the host and hospital environment.
Impact of COVID-19 on the mental health of US college students
Authors: Lee Jenny , Solomon Matthew , Stead Tej , Kwon Bryan , Ganti Latha 
Published: June 2021

Abstract
Background/aim
In the beginning of 2020, the novel Coronavirus disease (COVID-19) caused by the SARS-CoV-2 virus, became a public health emergency in the U.S. and rapidly escalated into a global pandemic. Because the SARS-CoV-2 virus is highly contagious, physical distancing was enforced and indoor public spaces, including schools and educational institutions, were abruptly closed and evacuated to ensure civilian safety. Accordingly, educational institutions rapidly transitioned to remote learning. We investigated the impacts of the COVID-19 pandemic on domestic U.S. college students, ages 18–24 years.

Methods
Through Pollfish®’s survey research platform, we collected data from 200 domestic U.S. college students in this age range (N = 200) regarding the physical, emotional, and social impacts of COVID-19 as well as key background information (e.g. whether or not they are first-generation or if they identify with the LGBTQIA+ community).

Results
Our results indicate that students closer to graduating faced increases in anxiety (60.8%), feeling of loneliness (54.1%), and depression (59.8%). Many reported worries for the health of loved ones most impacted their mental health status (20.0%), and the need to take care of family most affected current and future plans (31.8%). Almost one-half of students took to exercising and physical activity to take care of their mental health (46.7%). While a third did not have strained familial relationships (36.5%), almost one half did (45.7%). A majority found it harder to complete the semester at home (60.9%), especially among those who had strained relationships with family (34.1%). Seventy percent spent time during the pandemic watching television shows or movies. Significantly more men, first-generation, and low-income students gained beneficial opportunities in light of the pandemic, whereas their counterparts reported no impact. First-generation students were more likely to take a gap year or time off from school.

Conclusions
Although students found ways to take care of themselves and spent more time at home, the clear negative mental health impacts call for schools and federal regulations to accommodate, support, and make mental health care accessible to all students.
Biorefinery methods for extraction of oil and protein from rubber seed
Authors: Yang Miao , Zhu Wenlei , Cao Hui 
Published: June 2021

AbstractRubber seeds are a by-product of rubber production and are rich in oil and protein. Upgrading of rubber seeds to produce proteins, oils and feedstock can generate additional revenue for rubber production and reduce waste. The present study investigates the effects of different pre-treatments and extraction methods to determine the optimal methods to produce oil and protein from rubber seed kernels. Mechanical expulsion using a screw press and solvent extraction using n-hexane were employed for oil separation. The highest oil recovery efficiency of 95.12% was obtained using rubber seed meal that was pre-dried at 105 ℃. The sequential water–alkaline treatment was ideal for achieving high protein recovery while reducing the protein denaturation that can result from high operating temperatures and organic solvent contact. Over 90% of the total protein from rubber seed kernels could be recovered. Separating oil from kernels using hexane followed by protein extraction from the meals by enzymatic treatment provides a suitable method for comprehensive utilization of rubber seeds.
Evaluation of the bioformulation of potent native strains of Trichoderma spp. against the foot rot/gummosis of Kinnow mandarin
Authors: Choudhary Ajay Kumar , Singh Narinder , Singh Daljeet 
Published: June 2021

Abstract
Background
Foot rot/gummosis of citrus, caused by Phytophthora spp., is the most important disease of citrus, responsible for the yield losses of 10 to 30% in fruit production in major citrus growing countries. Considering the burden of disease, the present study was undertaken to evaluate different native potent strains of Trichoderma spp. against the foot rot/gummosis of citrus.

Results
Based on molecular characteristics, i.e., ITS ribosomal DNA sequence analysis, 3 isolates of Phytophthora nicotianae var. parasitica and 7 isolates of Trichoderma (6 isolates of T. asperellum and one of T. harzianum) were identified. Trichoderma isolates and Metalaxyl-M + Mancozeb were used to assess antagonistic activity against the foot rot/gummosis under field conditions. T. asperellum T20 mutant reduced gummosis lesion size (95.67%), improved feeder root density (0.0236 cm3), increased number (990.16), and yield of fruits (168.83 kg).

Conclusions
The talc-based bioformulation of T20 mutant (T. asperellum) strain, used as a soil treatment, was able to manage effectively the gummosis/foot rot of citrus and also exhibited the highest yield.
Assessment of production potential of long-linted Gossypium arboreum L. genotypes under different sowing times
Authors: Sankaranarayanan K. , Venugopalan M. V. , Kanjana D. 
Published: June 2021

Abstract
Background
Gossypium arboreum is one of the cultivated diploids which has inherent resistance against insect pests and diseases, can withstand moisture stress, needs less nutrients and is suitable for erratic, less rainfall and marginal soils. Due to its smaller boll size and poor locule retentivity, adoption of species by farmers is very low. So, best long-linted G. arboreum genotypes with high yielding potential and suitable sowing time will be identified for rainfed tracts of cotton cultivated areas in India.

Results
The pooled analysis results found that the significantly highest mean seed cotton yield was recorded with Phule Dhanwanthry (1069 kg/ha) which was on par with K12 (1027 kg/ha), DLSA 17 (977 kg/ha) and PA 812 (951 kg/ha). Planting at 4 August yielded the significantly higher seed cotton yield (1345 kg/ha) in comparison with 4 September sowing (536 kg/ha). The interaction results in pooled data revealed that Genotype PA 812 planted at 4 August registered the significantly highest seed cotton yield (1487 kg/ha) which was on par with all genotypes sown on 4 August except PA 402. Amongst long-linted genotypes, PA 760 recorded the significantly highest upper half length (29.9 mm), mean length (24.9 mm) and fibre strength (27.0 g/tex) and fibre quality index (349.7); which was on par with DLSA 17, PA 812 and PA 402. The quality characters had not been influenced significantly by different times of sowing.

Conclusions
The results concluded that the significantly highest mean seed cotton yield was recorded with Phule Dhanwanthry (1069 kg/ha) which was on par with K12 (1027 kg/ha), DLSA 17 (977 kg/ha) and PA 812 (951 kg/ha). Planting at 4 August found that the significantly higher seed cotton yield (1345 kg/ha) in comparison with 4 September sowing (536 kg/ha). Amongst long-linted genotypes, PA 760 recorded the significantly higher fibre quality index (349.7)
The effect of COVID-19 lockdown on psychiatric admissions: role of gender
Authors: Davies Monica , Hogarth Luke 
Published: June 2021

Background
The UK went into nationwide lockdown on 24 March 2020, in response to COVID-19. The direct psychiatric effects of this are relatively unknown.

Aims
We examined whether the first UK lockdown changed the demographics of patients admitted to psychiatric hospitals (to include gender, legality, route of admission and diagnoses), independent of seasonal variation..

Method
We conducted an anonymous review of psychiatric admissions aged ≥18 years in the 6-month period after the announcement of the first UK lockdown (March to August 2020), and in the previous year (March to August 2019), in Kent and Medway NHS and Social Care Partnership Trust in-patient facilities. The number of admissions were compared, along with factors that may help to explain the psychological effects of national lockdown.

Results
There was no significant increase in total number of admissions or the gender percentage. However, there was a 11.8% increase in formal sectioning under the Mental Health Act 1983. This increase was sustained and statistically significant across all 6 months. A sustained decrease in admissions via the crisis team was also observed as being statistically significant. Separate diagnoses saw changes in percentage of admissions between March and May. The most statistically significant was schizophrenia admissions for men in April (18.7%), and women in March (18.4%).

Conclusions
Our findings highlight the effect of COVID-19 on the legal status of psychiatric admissions, and emphasise the importance of having a robust, adaptable and open psychiatric service that caters to the ongoing needs of patients, regardless of government restrictions.
Boat noise interferes with Lusitanian toadfish acoustic communication
Authors: Alves Daniel , Vieira Manuel , Amorim M. Clara P. , Fonseca Paulo J. 
Published: June 2021

ABSTRACTAnthropogenic noise is considered a major underwater pollutant as increasing ocean background noise due to human activities is impacting aquatic organisms. One of the most prevalent anthropogenic sounds is boat noise. Although motorboat traffic has increased in the past few decades, its impact on the communication of fish is still poorly known. The highly vocal Lusitanian toadfish (Halobatrachus didactylus) is an excellent model to test the impact of this anthropogenic stressor as it relies on acoustic communication to attract mates. Here, we performed two experiments to test the impact of boat noise on the acoustic communication of the Lusitanian toadfish. Using the auditory evoked potential (AEP) technique, we first compared the maximum distance a fish can perceive a boatwhistle (BW), the mate attraction acoustic signal, before and after embedding it in boat noise. Noises from a small motorboat and from a ferryboat reduced the active space from a control value of 6.4–10.4 m to 2.0–2.5 m and 6.3–6.7 m, respectively. In the second experiment we monitored the acoustic behaviour of breeding males exposed to boat noise playbacks and we observed an increase in the inter-onset interval of BWs and a disruption of the usual vocal interactions between singing males. These results demonstrate that boat noise can severely reduce the acoustic active space and affect the chorusing behaviour in this species, which may have consequences in breeding success for individuals and could thus affect fitness.
The role of ligand-gated chloride channels in behavioural alterations at elevated CO2 in a cephalopod
Authors: Thomas Jodi T. , Spady Blake L. , Munday Philip L. , Watson Sue-Ann 
Published: June 2021

ABSTRACTProjected future carbon dioxide (CO2) levels in the ocean can alter marine animal behaviours. Disrupted functioning of γ-aminobutyric acid type A (GABAA) receptors (ligand-gated chloride channels) is suggested to underlie CO2-induced behavioural changes in fish. However, the mechanisms underlying behavioural changes in marine invertebrates are poorly understood. We pharmacologically tested the role of GABA-, glutamate-, acetylcholine- and dopamine-gated chloride channels in CO2-induced behavioural changes in a cephalopod, the two-toned pygmy squid (Idiosepius pygmaeus). We exposed squid to ambient (∼450 µatm) or elevated (∼1000 µatm) CO2 for 7 days. Squid were treated with sham, the GABAA receptor antagonist gabazine or the non-specific GABAA receptor antagonist picrotoxin, before measurement of conspecific-directed behaviours and activity levels upon mirror exposure. Elevated CO2 increased conspecific-directed attraction and aggression, as well as activity levels. For some CO2-affected behaviours, both gabazine and picrotoxin had a different effect at elevated compared with ambient CO2, providing robust support for the GABA hypothesis within cephalopods. In another behavioural trait, picrotoxin but not gabazine had a different effect in elevated compared with ambient CO2, providing the first pharmacological evidence, in fish and marine invertebrates, for altered functioning of ligand-gated chloride channels, other than the GABAAR, underlying CO2-induced behavioural changes. For some other behaviours, both gabazine and picrotoxin had a similar effect in elevated and ambient CO2, suggesting altered function of ligand-gated chloride channels was not responsible for these CO2-induced changes. Multiple mechanisms may be involved, which could explain the variability in the CO2 and drug treatment effects across behaviours.