bluuebunny/crossref_metadata_2025_split huggingface dataset:
Different DGAT1s show different TAG synthesis abilities and a specific amino acid substitution enhances oil accumulation
Authors: Hatanaka Tomoko , Tomita Yoshiki , Matsuoka Daisuke , Sasayama Daisuke , Fukayama Hiroshi , Azuma Tetsushi , Soltani Gishini Mohammad Fazel , Hildebrand David 
Published: September 2021

AbstractTriacylglycerols (TAGs) are the major component of plant storage lipids. Acyl-CoA:diacylglycerol acyltransferase (DGAT) catalyzes the final step of the Kennedy pathway, and responsible for plant oil accumulation. We previously found DGAT activity of Vernonia galamensis DGAT1 was distinctively higher than that of Arabidopsis thaliana DGAT1 and soybean DGAT1 in a yeast microsome assay. In this study, the DGAT1 cDNAs of Arabidopsis, Vernonia, soybean, and castor were introduced into Arabidopsis (ecotype Col-0). All Vernonia DGAT1 expressing lines showed a significantly higher oil content (average 49% relative increase compared to the wild type) followed by soybean, and castor. Most Arabidopsis DGAT1 over-expressing lines did not show a significant increase. In addition to these four DGAT1s, sunflower, Jatropha and sesame DGAT1 genes were introduced into the TAG biosynthesis defective yeast mutant (H1246). In the yeast expression culture, DGAT1s from Arabidopsis, castor, and soybean only slightly increased TAG content, however, DGAT1s from Vernonia, sunflower, Jatropha, and sesame remarkably increased TAG content more than 10 times higher than the former three DGAT1s. Three amino acid residues were characteristically common in the latter four DGAT1s. Using soybean DGAT1, these amino acid substitutions by site-directed mutagenesis was performed and analyzed. These substitutions substantially increased the TAG content.HighlightDGAT1s from several plant species were tested their TAG accumulation promotion in Arabidopsis and yeast. They were divided into high and low function and single amino acid substitution enhanced function
Opening the side exit pores of ClpP by lowering the pH of proteolytic chamber coupled with substrate hydrolysis
Authors: Kim Leehyeon , Lee Byung-Gil , Kim Min Kyung , Kwon Do Hoon , Kim Hyunmin , Brötz-Oesterhelt Heike , Roh Soung-Hun , Song Hyun Kyu 
Published: September 2021

AbstractThe ClpP serine peptidase is a tetradecameric degradation machine involved in many physiological processes. It becomes a competent ATP-dependent protease with Clp-ATPases. Small chemical compounds, acyldepsipeptides (ADEPs), are known to cause dysregulation and activation of ClpP without ATPases, and have potential as novel antibiotics. Previously, structural studies of ClpP from various species revealed the structural details, conformational changes, and activation mechanism. Although product release by the side exit pores has been proposed, the detailed driving force for product release remains elusive. Here, we report crystal structures of ClpP from Bacillus subtilis (BsClpP) in unforeseen ADEP-bound states. Cryo-electron microscopy structures revealed various conformational states at different pH conditions. To understand the conformational change for product release, we investigated the relationship between substrate hydrolysis and the pH lowering process. Our data, together with previous findings, provide insight into the molecular mechanism of product release by ClpP self-compartmentalizing protease.
SARS-CoV-2 preS dTM vaccine booster candidates increase functional antibody responses and cross-neutralization against SARS-CoV-2 variants of concern in non-human primates
Authors: Pavot Vincent , Berry Catherine , Kishko Michael , Anosova Natalie G. , Huang Dean , Tibbitts Tim , Raillard Alice , Gautheron Sylviane , Gutzeit Cindy , Koutsoukos Marguerite , Chicz Roman , Lecouturier Valerie 
Published: September 2021

AbstractThe emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants that partly evade neutralizing antibodies has raised concerns of reduced vaccine effectiveness and increased infection. We previously demonstrated in preclinical models and in human clinical trials that our SARS-CoV-2 recombinant spike protein vaccine adjuvanted with AS03 (CoV2 preS dTM-AS03) elicits robust neutralizing antibody responses in naïve subjects. Here, the objective was to document the potency of various booster vaccine formulations in macaques previously vaccinated with mRNA or protein subunit vaccine candidates.We show that one booster dose of AS03-adjuvanted CoV2 preS dTM, D614 (parental) or B.1.351 (Beta), in monovalent or bivalent (D614 + B.1.351) formulations, significantly boosted pre-existing neutralizing antibodies and elicited high and stable cross-neutralizing antibodies covering the four known SARS-CoV-2 variants of concern (Alpha, Beta, Gamma and Delta) and, unexpectedly, SARS-CoV-1, in primed macaques. Interestingly, the non-adjuvanted CoV2 preS dTM B.1.351 vaccine formulation also significantly boosted and broadened the neutralizing antibody responses.Our findings show that these vaccine candidates used as a booster have the potential to offer cross-protection against a broad spectrum of variants. This has important implications for vaccine control of SARS-CoV-2 variants of concern and informs on the benefit of a booster with our vaccine candidates currently under evaluation in phase 2 and 3 clinical trials (NCT04762680 and NCT04904549).
A genetic compensatory mechanism regulated by c-Jun and Mef2d modulates the expression of distinct class IIa HDACs to ensure peripheral nerve myelination and repair
Authors: Velasco-Aviles Sergio , Patel Nikiben , Casillas-Bajo Angeles , Frutos-Rincón Laura , Velasco-Serna Enrique , Gallar Juana , Arthur-Farraj Peter , Gomez-Sanchez Jose A. , Cabedo Hugo 
Published: September 2021

ABSTRACTThe class IIa histone-deacetylases (HDACs) have pivotal roles in the development of different tissues. Of this family, Schwann cells express HDAC4, 5 and 7 but not HDCA9. Here we show that a transcription factor regulated genetic compensatory mechanism within this family of proteins, blocks negative regulators of myelination ensuring peripheral nerve developmental myelination and remyelination after injury. Thus, when HDAC4 and 5 are knocked-out from Schwann cells, a c-Jun dependent mechanism induces the compensatory overexpression of HDAC7 permitting, although with a delay, the formation of a myelin sheath. When HDAC4,5 and 7 are simultaneously removed, the Myocyte- specific enhancer-factor d (Mef2d) binds to the promoter and induces the de novo expression of HDAC9, and although several melanocytic- lineage genes are mis- expressed and Remak bundle structure is disrupted, myelination proceeds after a long delay. Thus, our data unveil a finely tuned compensatory mechanism within the class IIa HDAC family, coordinated by distinct transcription factors, that guarantees the ability of Schwann cells to myelinate during development and remyelinate after nerve injury.
A simple immunoassay for extracellular vesicle liquid biopsy in microliters of non-processed plasma
Authors: Campos-Silva Carmen , Cáceres-Martell Yaiza , Sánchez-Herrero Estela , Condado Amaia Sandúa , Beneitez-Martínez Alexandra , Hernández Álvaro González , Provencio Mariano , Romero Atocha , Jara Ricardo , Yáñez-Mó María , Valés-Gómez Mar 
Published: September 2021

AbstractExtracellular vesicles (EVs), released by most cell types, provide an excellent source of biomarkers in biological fluids. Here we describe a method that, using just a few microliters of patient’s plasma, identifies tumour markers exposed on EVs. Studying physico-chemical properties of EVs in solution, we demonstrate that they behave as stable colloidal suspensions and therefore, in immunocapture assays, many of them are unable to interact with a stationary functionalised surface. Using flocculation methods, like those used to destabilize colloids, we demonstrate that cationic polymers increase EV ζ-potential, diameter, and sedimentation coefficient and thus, allow a more efficient capture on antibody-coated surfaces by both ELISA and bead-assisted flow cytometry. These findings led to optimization of a protocol in microtiter plates allowing effective immunocapture of EVs, directly in plasma without previous ultracentrifugation or other EV enrichment. The method, easily adaptable to any laboratory, has been validated using plasma from lung cancer patients in which the epithelial cell marker EpCAM has been detected on EVs. This high throughput, easy to automate, technology allows screening of large numbers of patients to phenotype tumour markers in circulating EVs, breaking barriers for the validation of proposed EV biomarkers and the discovery of new ones.
Acoustic Force Spectroscopy Reveals Subtle Differences in Cellulose Unbinding Behavior of Carbohydrate-Binding Modules
Authors: Hackl Markus , Contrada Edward V. , Ash Jonathan E. , Kulkarni Atharv , Yoon Jinho , Cho Hyeon-Yeol , Lee Ki-Bum , Yarbrough John M. , Chundawat Shishir P. S. 
Published: September 2021

AbstractTo rationally engineer more efficient cellulolytic enzymes for cellulosic biomass deconstruction into sugars for biofuels production, it is necessary to better understand the complex enzyme-substrate interfacial interactions. Carbohydrate binding modules (CBM) are often associated with microbial surface-tethered cellulosomal or freely secreted cellulase enzymes to increase substrate accessibility. However, it is not well known how CBM recognize, bind, and dissociate from polysaccharide surfaces to facilitate efficient cellulolytic activity due to the lack of mechanistic understanding of CBM-substrate interactions. Our work outlines a general approach to methodically study the unbinding behavior of CBMs from model polysaccharide surfaces using single-molecule force spectroscopy. Here, we apply acoustic force spectroscopy (AFS) to probe a Clostridium thermocellum cellulosomal scaffoldin protein (CBM3a) and measure its dissociation from nanocellulose surfaces at physiologically relevant, low force loading rates. An automated microfluidic setup and methodology for uniform deposition of insoluble polysaccharides on the AFS chip surfaces is demonstrated. The rupture forces of wild-type CBM3a, and its Y67A mutant, unbinding from nanocellulose surface suggests distinct CBM binding conformations that can also explain the improved cellulolytic activity of cellulase tethered to CBM. Applying established dynamic force spectroscopy theory, the single-molecule unbinding rate at zero force is extrapolated and found to agree well with bulk equilibrium unbinding rates estimated independently using quartz crystal microbalance with dissipation monitoring. However, our results highlight the limitations of applying classical theory to explain the highly multivalent CBM-cellulose interactions seen at higher cellulose-CBM bond rupture forces (>15pN).Significance StatementCellulases are multi-modular enzymes produced by numerous microbes that catalyze cellulose hydrolysis into glucose. These enzymes play an important role in global carbon cycling as well as cellulosic biofuels production. CBMs are essential components of cellulolytic enzymes involved in facilitating hydrolysis of polysaccharides by tethered catalytic domains (CD). The subtle interplay between CBM binding and CD activity is poorly understood particularly for heterogeneous reactions at solid-liquid interfaces. Here, we report a highly multiplexed single-molecule force spectroscopy method to study CBM dissociation from cellulose to infer the molecular mechanism governing substrate recognition and dissociation. This approach can be broadly applied to study multivalent protein-polysaccharide binding interactions relevant to other carbohydrates such as starch, chitin, or hyaluronan to engineer efficient biocatalysts.
DNA metabarcoding Uncover the Diet of Subterranean Rodents in China
Authors: Zhang Xuxin , Zou Yao , Nan Xiaoning , Han Chongxuan 
Published: September 2021

AbstractObjectiveIn the past, the zokor, which lived in northern China, caused great harm to agriculture and forestry production due to its large and sophisticated diet. Since the rat lives underground for most of its life, researchers know little about its dietary habits. Further understanding of its diet in the field is of important meaning for developing green and sustainable control strategies for the rat.MethodsLongde County in Liupan Mountain area of Ningxia Hui Autonomous Region was selected as the interest area to capture zokor and investigate the species of habitat plants.We selected chloroplast trnL gene and eukaryotic internal transcription spacer 1 (ITS 1) primers to amplify DNA from the gastric contents of zokor,and then sequenced on Illumina Miseq PE300 platform.ResultsThe gastric contents of Eospalax smithii (n=16) and E.cansus(n=9) were analyzed by operational taxonomic units (OTU) clustering and amplicon sequence variants(ASVs).The OTU clustering method obtained 2,995 OTUs, and the ASV method obtained 4,657 ASVs. The ASV method was better than the OTU clustering method, and the ASV method was adopted in the subsequent analysis. The food list of 32 families, 80 genera and 154 species was obtained by ASV method after the error was removed. The food composition of zokor was evaluated by relative abundance(%RA) method and frequency of occurrence(%ROO) method. At the Family level, it was found that zokor mainly fed on Asteraceae, Poaceae, Rosaceae, Pinaceae, Brassicaceae, Apiaceae, etc. At the Genus level, they are mainlyEchinops, Littledalea,Artemisia,Picea, Cirsium, Elymus and so on. The diet alpha diversity of E.cansus was slightly higher than that of E.smithii (P > 0.05). The correlation coefficient between Sobs index of alpha diversity and body weight of zokor was −0.382 (P = 0.059). The diet beta diversity proved that most zokors (22/25) clustered together, with low heterogeneity. They fed positively on Calamagrostis, Cirsium, Echinops, Medicago, Sanguisorba and Taraxacum. We found that zokor mainly fed on the roots of perennial herbs(PH), which were rich in water, carbohydrate, fat and protein, which provided an important energy source for its survival.ConclusionHigh-throughput sequencing(HTS) based DNA metabarcoding technology has well revealed the diet of zokor, which are generalist.
Exploring information seeking of rural older adults during the COVID-19 pandemic
Authors: Lund Brady , Ma Jinxuan 
Published: September 2021

PurposeThis study investigates the impact of the COVID-19 pandemic on the types and sources of information sought by older adults along with their motivations in the Midwestern United States.Design/methodology/approachInterviews were conducted with 30 older adults residing in rural communities around the Midwestern United States during late-summer (July/August) 2020, using a protocol based on Dervin's Sense-Making Methodology. The resulting data was analyzed using standard content analysis procedures, guided by the theoretical frameworks based on Dervin's Sense-Making and Williamson's Ecological Model of Information Behavior. Implications of COVID-19 for the normative behaviors described in these models are discussed.FindingsFindings show that older adults were concerned primarily with health and political information during this period, but that this information was not necessarily sought only to address an informational need, but also to satisfy the need to maintain social and emotional connections in coping with isolation and loneliness. Sources of information that allowed for social interaction with people were favored. Wider personal networks (community members) were strained by the social distancing measures and closures. These findings have theoretical and practical implications for considering the impact of social restrictions on information seeking behaviors of older adults in a time of crisis.Originality/valueThis study is the first, known to the authors, that applied the two adopted theoretical frameworks to explore information seeking behaviors of older adults during the COVID-19 pandemic.
The characteristics and evolution of business model for green buildings: a bibliometric approach
Authors: Zhao Xiaojing , Pan Wei 
Published: September 2021

PurposeThe wide uptake of green building is hindered by factors such as limited market demand and high up-front cost despite substantial policy incentives. Successful business models (BMs) help facilitate the diffusion of sustainable innovation and have attracted increasing attention. However, little attention has been paid to examining the adoption of green building from the perspective of BM. This paper aims to identify the characteristics of BM that companies adopted to deliver green building projects and examine the evolution path of BM for green buildings.Design/methodology/approachA total of 744 interdisciplinary bibliographic records related to BM and green buildings from the Web of Science Core Collection database have been identified from the Web of Science Core Collection and analyzed through co-author analysis, co-word analysis and co-citation analysis.FindingsBM research mainly focuses on five subjects, namely, environmental science, engineering, business, economics, science and technology. Keywords “strategy”, “capability”, “perspective” and “design” receive the highest centrality index, which suggests that the strategy design and corporate capability are indispensable elements when designing or innovating a company's BM. “BM canvas” and “resource based view” received the burst citations in the recent decade. Twelve research clusters are identified, including performance evaluation, waste management, energy efficiency, BM, project business, professional capital, leadership and product innovation. These topics highlight three evolution direction for green building projects, i.e. life cycle-oriented value proposition, collaborative value delivery and innovative cost and revenue structure.Originality/valueThe findings contribute to a systematic understanding of the underlying mechanisms of BM for green buildings and illuminate the development trend of business strategies for sustainability.
Improving women's energy access, rights and equitable sustainable development: a Ghanaian perspective
Authors: Owusu-Manu De-Graft , Sackey David Mensah , Osei-Asibey Dickson , Kyerewah Agyapong Rachelle , John Edwards David 
Published: September 2021

PurposeThe purpose of the study is to investigate the challenges in improving women's energy access, rights and equitable sustainable development from a Ghanaian perspective.Design/methodology/approachThe research utilizes a mixed method. A qualitative in-depth exploratory design was chosen to understand how gender is mainstreamed within Ghana's energy sector. This included semi-structured interviews with key managers, experience policy experts and focus groups. The semi-structured interviews were analyzed using thematic content analysis (TCA).FindingsThe study reveals that the National Energy Policy of 2010, as the main energy policy regulating the energy sector in Ghana, does make provision for gender equality, safety especially women, in line with Ghana's sustainable development goals. The energy policy aims to empower women and create gender parity in the sector. Nevertheless, the study also found major challenges to gender mainstreaming in the energy sector, including poor analysis in formulating energy policies, inadequate financial resources, and poor monitoring and evaluation.Originality/valueThe paper exposes gender equity challenges associated with the energy sector in Ghana. It also offers a new policy angle which connects gender mainstreaming to sustainable development. The research describes how women are included in developing energy policies and in addressing gender challenges in the energy sector.
The influence of altruistic leadership behavior and learning culture on work–family relationship in Chinese SMEs
Authors: Xie Lei , Qiu Shaoping , Biggs Mary Jo Garcia 
Published: September 2021

Purpose
This study aims to examine the relationship between supervisor’s altruistic leadership behavior (ALB) and family-to-work development (FWD) in the context of Chinese small and medium-sized enterprises (SMEs); and test the mediation effect of learning organization culture (LOC) between ALB and FWD.

Design/methodology/approach
A non-experimental, quantitative, cross-sectional survey research approach was applied. A structural equation modeling technique was used to test all hypotheses.

Findings
Results indicated that ALB is positively and significantly correlated with FWD. Additionally, the relationship between ALB and FWD is mediated by LOC.

Practical implications
This study suggests that building and maintaining an effective LOC requires leaders to champion, human resource (HR) professionals to strengthen and employees to support learning as a system. Thus, HR professionals should implement altruistic leadership mentoring and coaching programs. In the case of Chinese SMEs, altruistic leaders are especially instrumental in generating followers’ positive outcomes in both work and family domains.

Originality/value
This study looks into the influence of organizational factors on the direction of FWD. In particular, this study seeks to examine organizational factors that relate to spillover from family to work.
Impact of anthropomorphic features of artificially intelligent service robots on consumer acceptance: moderating role of sense of humor
Authors: Zhang Mengying , Gursoy Dogan , Zhu Zhangyao , Shi Si 
Published: September 2021

Purpose
This study aims to investigate the impact of both physical and personality-related anthropomorphic features of an artificial intelligence service robot on the cognitive and affective appraisals and acceptance of consumers during service delivery.

Design/methodology/approach
The proposed hypotheses that investigate the effects of service robots’ physical appearance on the emphasis consumers place on each evaluation criteria they use in determining their willingness to accept the use of service robots in service delivery and the moderating role of sense of humor are tested by conducting two studies using scenario-based experiments.

Findings
The results show that humanlike appearance leads to higher performance expectancy, mascot-like appearance generates higher positive emotions and machine-like appearance results in higher effort expectancy. The effects of humanlike and mascot-like appearances on consumer acceptance are moderated by the sense of humor of service robots. However, the sense of humor effect is attenuated with a machine-like appearance owing to the lack of anthropomorphism.

Practical implications
This study provides crucial insights for hospitality managers who plan to use service robots in service delivery. The findings highlight the key roles of appearance type and sense of humor of service robots in influencing the appraisals and acceptance of consumers regarding the use of service robots in service delivery.

Originality/value
This study focuses on comparing the effects of traditional and mascot-like appearances of service robots on consumer appraisals and identifies sense of humor as a cute anthropomorphized personality trait of service robots.