bluuebunny/crossref_metadata_2025_split huggingface dataset:
RIPK1/RIPK3-Mediated Necroptosis is Involved&nbsp;in Sevoflurane-Induced Neonatal Neurotoxicity in The Rat Hippocampus.
Authors: Xu Rui , Zhu Yun , Jia Jie , Li Wenxian , Lu Yi 
Published: February 2021

Abstract
Purpose: Recent studies have shown that exposure to sevoflurane in developing brains causes neuronal apoptosis and cognitive dysfunction. “Necroptosis” is a novel pathway of necrosis. We introduced the caspase-specific inhibitor Z-VAD in addition to the receptor-interacting protein kinase 1 (RIPK1) inhibitor Nec-1, to ascertain the existence and importance of necroptosis.Methods: Sprague–Dawley rat pups postnatal day 7 were randomly assigned into one of four groups: control, sevoflurane+Z-VAD, sevoflurane+Z-VAD+Nec-1 and 3% sevoflurane group. Neuronal apoptosis was evaluated by hematoxylin and eosin staining . The MTT assay was performed to evaluate cell viability. Immunofluorescence was employed to measure expression of RIPK1 and RIPK3. Western blots showing expression of RIPK1 and RIPK3 were used to explore the role of necroptosis. Binding of RIPK1/RIPK3 was detected via co-immunoprecipitation. Finally, the Morris water maze test was used to determine cognitive function.Results: Exposure to 3% sevoflurane for 6 hours induced neurotoxicity and inhibited cell viability. Neuron viability was low in the SEV and SEV +Z-VAD groups. The study revealed that RIPK1 and RIPK3 protein expression increased significantly, but there was no significant differences between the SEV and SEV+Z-VAD groups. Co-immunoprecipitation results showed that sevoflurane exposure enhanced binding of RIPK1/RIPK3 protein significantly. Blockade of apoptosis and necroptosis alleviated sevoflurane-induced cognitive impairment.Conclusions: Sevoflurane exposure elicited neurotoxicity within neonatal hippocampal neurons and tissues. Blockade of apoptosis alone did not attenuate sevoflurane-induced neurotoxicity (SIN). RIPK1/RIPK3-mediated necroptosis was involved in SIN in hippocampal neurons. SIN could be attenuated only by inhibiting apoptosis and necroptosis.
Sustainable tire supply chain network design and operations planning using a multi objective , scenario-based optimization approach
Authors: Zenouz Reza Yousefi , Jamalnia Aboozar , Farrokh Mojtaba Farrokh , Asadi Mastaneh 
Published: February 2021

Abstract
Each year, millions of tires reach their end of life. Worn-out tires are either buried or burned, both of which harm the environment through polluting the air and groundwater. Companies need to consider their social responsibility, such as employment and regional development, and the environmental impact of their activities when making strategic and operational decisions. This study addresses the closed-loop supply chain network design (SCND) and operations planning problem with regard to the three dimensions of sustainability using a mathematical programming approach. The options of retreading, recycling, and energy recovery together with the use of green technologies are considered to minimize the environmental impacts. The proposed decision model can help supply chain managers in tire manufacturing industry make better-informed decisions in order to achieve the three-fold objectives of sustainability. The developed mathematical model turns out to be a multi-objective, multi-echelon, and multi-product mixed integer linear programming. The model is solved using the Lp-metric method and CPLEX solver. The scenario approach is used to address the uncertainty in demand of new products and the rate of return of worn-out tires. The model solutions are the optimal location of the facilities considering population density and unemployment rate in addition to economic dimension, the optimal amount of allocation, the flow of materials, and the best green technology selection. Sensitivity analysis is also conducted to validate the model and test the robustness of the obtained solutions. Finally, managerial implications are provided.
The effect of MQL on tool wear progression in low-frequency vibration-assisted drilling of CFRP/Ti6Al4V stack material
Authors: Hussein Ramy , Sadek Ahmad , Elbestawi Mohamed , Attia Helmi 
Published: February 2021

Abstract

In this paper, the tool wear mechanism in low-frequency vibration-assisted drilling (LF-VAD) of carbon fiber reinforced polymer (CFRP)/Ti6Al4V stacks has been proposed using variably machining parameters. Based on the kinematics analysis, the effect of vibration amplitude on the chip formation, uncut chip thickness, chip radian, and axial velocity was presented. Subsequently, the effect of LF-VAD on the cutting temperature, tool wear, delamination, and geometrical accuracy was presented for different vibration amplitude. The LF-VAD with the utilization of minimum quantity lubricant (MQL) resulted in a successful drilling process of 50 holes, with a 63 % reduction of the cutting temperature. For the rake face, LF-VAD reduced the adhered height of Ti6Al4V by 80 % at low cutting speed and reduced the crater depth by 33 % at the high cutting speed. On the other hand, LF-VAD reduced the flank wear land by 53 %. Furthermore, LF-VAD showed a significant enhancement on the CFRP delamination, geometrical accuracy, and burr formation.
Transcription Factor C-Jun Modulates GLUT1 in Glycolysis and Breast Cancer Metastasis
Authors: Zhu Ping , Lu Jingjing , Zhou Yue , Gao Yabiao , Chen Shuyi , Yu Jerry , Sun Yangbai , Zhou Ping 
Published: February 2021

Abstract
As one of the main isoforms of membranous glucose transporters (GLUT), GLUT1 involves tumorigenesis, metastasis and prognosis in a variety of cancers. However, its role in breast cancer metastasis remains to be elucidated. Here we examined its transcriptional and survival data in patients with breast cancer from several independent databases including the Oncomine, Gene Expression Profiling Interactive Analysis, Gene Expression across Normal and Tumor tissue, UALCAN, cBioPortal, Kaplan-Meier Plotter and PROGgeneV2. We found that its mRNA expression was significantly high in cancer tissues, which was associated with metastasis and poor survival. Transcription factor c-Jun might bind to GLUT1 promoter to downregulate its gene expression or mRNA stability, therefore to suppress glycolysis and metastasis. By qRT-PCR, we verified that GLUT1 was significantly increased in 38 paired human breast cancer samples while JUN was decreased. Furthermore, the protein level of GLUT1 was higher in tumor compared to normal tissues through IHC assay. We also performed GO and KEGG analysis of genes related to GLUT1 and JUN to explore underlying pathways. Our results further support that transcription factor c-Jun regulates GLUT1 to influence glycolysis and breast cancer metastasis.
Software Evaluation for De Novo Detection of Transposons
Authors: Rodríguez Matias , Makalowski Wojciech 
Published: February 2021

Abstract
Transposable elements (TEs) are major genomic components in most eukaryotic genomes and play an important role in genome evolution. However, despite their relevance the identification of TEs is not an easy task and a number of tools were developed to tackle this problem. To better understand how they perform, we tested several widely used tools for de novo!TE detection and compared their performance on both simulated data and well curated genomic sequences. The results will be helpful for identifying common issues associated with TE-annotation and for evaluating how comparable are the results obtained with different tools.
Antimicrobial Activity Study of Chinese Medicine Madeng’ai
Authors: Tang Zhenrong , Zhao Yannan , Zhang Zaiqi , Yue Huan , Wang Dan , Liu Shengchun , Tang Hua 
Published: February 2021

Abstract
Background Due to the overuse of antibiotics, many multidrug-resistant bacteria have emerged, which brings huge challenges to the clinical treatment of bacterial infections. New products for anti-infection are necessary. Methods Madeng’ai powder was added with Milli-Q water or LB culture and autoclaved to prepare medicine suspension at different concentration. Bacteria were cultured in LB with different concentration of Madeng’ai. and swab on LB agar plates to get minimal inhibitory concentration (MIC) of Madeng’ai. Mice back was cut to make wound and MRSA/PAE suspension was injected in the wound area. Then swab with Madeng’ai extracts. Bacteria growth of infected secretions was checked on LB agar, and Hematoxylin and eosin (H&E) staining was performed for Histological analysis of skin tissues infected with bacteria after Madeng’ai and PBS (control) treatment. Results Madeng’ai could widely inhibit E.faecalis, Pseudomonas aeruginosa (PAE), Klebsiella pneumoniae (K.pneumoniae) and Acinetobacter baumannii (A.baumannii) at concentration of 4.0 mg/ml. The mice model also showed that Madeng’ai had imposed restrictions on MRSA and PAE growth in vivo. Conclusion Here, we report that a new Chinese medicine Madeng’ai has antimicrobial activity functions in vitro and in vivo. These data briefly showed that Madeng’ai functioned on antimicrobial and provided a new consideration for an antibiosis product.
Long noncoding RNA ANRIL Knockdown Attenuates Neuroinflammation Following Ischemic Stroke via Suppressing the Expression of NF-κB In Vitro and In Vivo
Authors: Dong Zhi , Deng Ling , Guo Yi , Liu Jingdong , Chen Sha , Wang Xuan , Zhao Hongxia , Zuo Tianrui , Hu Qingwen 
Published: February 2021

Abstract
Increasing evidence suggests that long noncoding RNAs can exert neuroprotective effects in cerebral ischemia-reperfusion injury. Levels of the long noncoding RNA ANRIL (ANRIL) are reportedly altered in ischemic stroke (IS) patients, but its role in IS requires further clarification. This study was designed to explore the mechanistic function of ANRIL in IS. In vitro, HT22 cells was treated with an oxygen-glucose deprivation/reperfusion (OGD/R). In vivo, brain ischemia/reperfusion was induced by 60-minute transient middle cerebral artery occlusion/ reperfusion (MCAO/R) IS model in C57/BL6 mice. Additionally, cells were transfected with si-ANRIL, pcDNA3.1-ANRIL, pcDNA3.1-NF-κB, or appropriate negative controls, and si-ANRIL and pcDNA3.1-NF-κB were administered into the lateral ventricles in MCAO/R model mice. Cell viability and apoptosis were detected via MTT and flow cytometry assays. mRNA and protein expression of NF-κB were detected via qRT-PCR and Western blotting. IL-1β, IL-6, TNF-a, and iNOS levels were detected via ELISA. In addition, infarcted area and neuronal injury were evaluated via TTC, Nissl, and immunofluorescent staining. We found that ANRIL knockdown increased cell viability and reduced apoptosis in vitro. Additionally, we found that ANRIL knockdown decreased p-P65, P65, IL-1β, IL-6, TNF-a, and iNOS levels, whereas these effects were reversed by NF-κB overexpression both in vitro and in vivo. Our results suggest that ANRIL knockdown attenuates neuroinflammation by suppressing the expression of NF-κB both in vitro and vivo model of IS, sugguesting that ANRIL might be a potentially viable therapeutictarget to diminish neuroinflammation in IS patients.
Willingness of Households to Pay Community Based Health Insurance and its Associated Factors in Mecha District, Northwest Ethiopia: Community based Cross-sectional Study
Authors: Demeke Getaneh Bizuayehu 
Published: February 2021

Abstract
Background

Community-based health insurance schemes helps to give financial protection and decrease direct out- of-pocket payment for health care based on the assumption of risk-pooling and community solidarity to risks of falling sick. Ethiopia is a low income country with more of health spending out of pocket payment by households. Community based health insurance was introduced in Ethiopia in 2010.It covers only the rural community and informal sector.
Objectives

this study aimed to assess willingness of households to pay community based health insurance and its associated factors in Mecha district, Northwest, Ethiopia.
Methods

Community based cross-sectional study design was used to collect data from 285 household heads using multistage sampling techniques in Mecha district Northwest Ethiopia. The data were collected by using trained data collectors and using a pre-tested structured questionnaire. A binary logistic regression model was used to determine the presence of statistically significant associations between the dependent and independent variables at p-value < 0.05 and AOR values with 95% CI.
Results

From the total of 296 sampled respondents, 285 participated in the study with the response rate of 96.3%. Of these, 251(88.1%) were willing to join voluntary as well as 34(11.9%) were join mandatory and 256(89.8%) of them were willing to pay community based health insurance services. The average amount of money the households were willing to pay per household per annum was 334.02 ETB found with the interval of (317.32–351.30) with the range between 240–1000 ETB.
Conclusions

The willingness of house hold heads to pay for the community-based health insurance was high. Residence, join CBHI, premium affordable, CBHI have an advantage and distance from households home to HF were more willing to pay CBHI schemes. The study indicated that high willing to pay and low CBHI package fulfil the needs of HH treatment as well as overall CBHI service level was poor. Therefore, Mecha district CBHI coordinating office should be scale up the community-based health insurance services in the scheme.
The relationship between demographic features, anthropometric parameters, sleep hours, and physical activity and ECG parameters in Fasa Persian cohort study
Authors: Mirahmadizadeh Alireza , Farjam Mojtaba , Sharafi Mehdi , Fatemian Hossein , Kazemi Maryam , Geraylow Kiarash Roustai , Dehghan Azizallah , Amiri Zahra , Afrashteh Sima 
Published: February 2021

Abstract
Backgrounds: Cardiovascular Diseases (CVDs) are the first leading cause of death worldwide. The present study aimed to investigate the relationship between demographics, anthropometrics, sleep time, and physical activity and ECG parameters in Fasa Persian cohort study. Methods: In this cross-sectional study, the basic information of 10,000 participants aged 35-70 years in Fasa cohort study was used. The information used in this study included demographic data, main Electrocardiogram (ECG) parameters, anthropometric data, sleep time, and physical activity. Data analysis was performed using t-test, chi-square, and linear regression model. Results: The findings of this study suggested that increased age was significantly associated with all study parameters. Nevertheless, gender and body mass index showed no significant relationship with SV3 and PR. Wrist circumference، hip circumference, and waist circumference significantly increased the mean values of the ECG parameters. However, sleep time was not significantly associated with the ECG parameters. In addition, hypertension was a major comorbidity, which was shown to increase the mean values of the ECG parameters. Conclusion: A number of factors affected the ECG parameters. Thus, in order to interpret ECGs, in addition to age and gender, anthropometric indices, physical activity, and previous history of comorbidities, such as hypertension and ischemic heart disease, should be taken into consideration.
WITHDRAWN: Development of ceramide and honey based biodegradable hydrogel dressing’s materials for the application to burn skin
Authors: Rana S. M. Masud , Ashrafuzzaman Md. 
Published: February 2021

Abstract
Ceramide is well known for its skin barrier function where hydrogel is an ideal one for wound healing, on the other hand honey is used for antibacterial activity and burn wound treatment from many years ago. So our aim to develop a ceramide loaded honey based hydrogel biomaterial for burn wound healing. The biomedical hydrogel biomaterial have been prepared by solution casting method. The developed Hydrogel membranes were characterized by using Fourier transform infrared spectroscopy (FT-IR), field emission scanning electron microscopy (FESEM) and UV- Vis Spectrophotometer. Besides that, swelling behavior in physiological solution, moisture retention capability, gel fraction, water vapor transmission rate (WVT), porosity and antimicrobial study were also evaluated. After all the hydrogel were evaluated for burn wound healing activity in mice model. Ceramide Hydrogel (C2) & Ceramide & Honey Hydrogel (CH) showed significant wound healing rate but only CH hydrogel showed significant antibacterial activity against Staphallycoccus aureus bacteria. Other physicochemical, swelling, WVT, porosity, gel fraction, weight loss, and FTIR, FESEM & UV study was very satisfactory and all experimented data strongly support the use of the hydrogel membrane as a burn wound healing.
Preparation of microcrystalline cellulose from Rabdosia rubescens and study on its membrane properties
Authors: Wei Tong , Li Meng , Shi Wenrui , Liang Zhengyong 
Published: February 2021

Abstract
Microcrystalline cellulose (MCC) from Rabdosia rubescens was prepared by acid hydrolysis, and the corresponding yield of MCC was about 94% when the hydrochloric acid concentration was 10%, the acid hydrolysis time was 70min and the acid hydrolysis temperature was 70℃. The MCC was dissolved in N-methylmorpholine-N-oxide(NMMO)by phase transformation method to prepare MCC membrane. The structure and mechanical properties of MCC membrane were studied systemically. The results showed that the cellulose structure changed from type I to type II in the process of membrane formation, and the thermal stability also decreased. The content of MCC in the casting solution has a great influence on the mechanical properties of the membranes. The higher the content of MCC, the better the comprehensive mechanical properties of the membranes. When the MCC content is 9%, the tensile strength is 8.38 MPa and the elongation at break is 26.72%. Finally, the separation properties of membrane studed by separation bovine serum albumin (BSA) from water. The results showed that the rejection rate and water flux changed positively and negatively with the change of MCC content. When the content was 5%, MCC membrane showed the best comprehensive performance, its rejection of BSA was 37.23 g/(m2·h), and the corresponding rejection rate and water flux were 88.87% and 41.89 L/(m2·h) respectively.
Disparate Genetic Variants Associated With Distinct Components of Cowpea Resistance to the Seed Beetle Callosobruchus Maculatus
Authors: Messina Frank J , Lish Alexandra M , Gompert Zachariah 
Published: February 2021

Abstract
Cowpea ( Vigna unguiculata ) is an important grain and fodder crop in arid and semi-arid regions of Africa, Asia, and South America, where the cowpea seed beetle, Callosobruchus maculatus , is a serious post-harvest pest. Development of cultivars resistant to C. maculatus population growth in storage could increase grain yield and quality and reduce reliance on insecticides. Here, we use a MAGIC (multi-parent, advanced-generation intercross) population of cowpea consisting of 305 recombinant inbred lines (RILs) to identify genetic variants associated with resistance to seed beetles. Because inferences regarding the genetic basis of resistance may depend on the source of the pest or the assay protocol, we used two divergent geographic populations of C. maculatus and two complementary assays to measure several aspects of resistance. Using polygenic genome-wide association mapping models, we found that the cowpea RILs harbor substantial additive-genetic variation for most resistance measures. Variation in several components of resistance, including larval development time and survival, was largely explained by one or several linked loci on chromosome 5. A second region on chromosome 8 explained increased seed resistance via the induction of early-exiting larvae. Neither of these regions contained genes previously associated with resistance to insects that infest grain legumes. We found some evidence of gene-gene interactions affecting resistance, but epistasis did not contribute substantially to resistance variation in this mapping population. The combination of mostly high heritabilities and a relatively consistent and simple genetic architecture increases the feasibility of breeding for enhanced resistance to C. maculatus.
Simultaneous in Situ Detection of Protein Expression of Multiple Tumor Markers of CTC and Heteroploid of Chromosome 8 in Primary Lung Cancer
Authors: Yao Hanqing , Wang Zhengdong , Yang Junjun , Xu Xingxiang , Song Yong 
Published: February 2021

Abstract
Background

Past studies have shown that circulating tumor cells (CTCs) play an important role in the clinical staging, efficacy monitoring and prognosis evaluation of lung cancer.
Methods

In this study we investigated the expression of CK18 and Vimentin on the surface of CTC and the aneuploidy of chromosome 8 in peripheral blood of 24 patients with metastatic primary lung cancer which were detected by subtraction enrichment and immunofluorescence in situ hybridization (SE-iFISH), and their correlation with clinicopathological features, curative effect and prognosis was analyzed.
Results

The positive rate of CTC was 95.83% (23/24). There was a certain correlation between the positive rate of CTC and smoking and a correlation between the number of CTC and the histopathological type of patients. The number of monoploid, diploid, triploid and polyploid CTC had no statistical correlation with progression-free survival (PFS) or overall survival (OS). However, a tetraploid CTC count of two or more was an unfavorable predictor of response and a tetraploid CTC count ≥ 1 is an unfavorable prognostic predictor of poor OS. The positive rate of CK18 + CTC in all 24 patients was 4.35% (1/23). Meanwhile, 7 out of these 24 patients were also tested for Vimentin, and the positive rate of Vimentin + CTC was 85.71% (6/7). Small-cell (≤ 5µm) CTC was found in 6 of these 7 patients and it accounted for 11.83% (11/93) of the total CTC. The tumor marker phenotype of small-cell CTC was CK18 - Vimentin +. In addition, circulating tumor microthrombi (CTM) were found in 2 of these 7 patients (28.57%).
Conclusion

SE-iFISH has a high detection rate of CTC in peripheral blood of patients with metastatic primary lung cancer, and it can identify small-cell CTC. Tetraploid CTC count ≥ 2 can predict poor PFS in patients with advanced lung cancer. Tetraploid CTC count ≥ 1 may predict poor OS in patients with advanced lung cancer. CTM can predict poor prognosis in patients with lung cancer.
The Inherent Relationship between Carbon Nanodots and Carbon Source in the Determination of Metal Ions
Authors: Chen Manling , Liu Xue 
Published: February 2021

Abstract
Carbon nanodots (CDs) have exhibited excellent sensing capability for various metal ions. However, it is difficult to determine the selectivity of CDs to metal ions. In this work, we chose appropriate carbon source to design CD sensors against Cu(II) and Ag(I). Glycine, histidine and leucine have been confirmed to form complexes with Cu(II) and Ag(I), and were applied to prepare CDs using microwave heating method. The as-prepared CDs inherited the specific ion-binding capability from their carbon source and could response to both Cu(II) and Ag(I). The response sensitivity corresponded to the binding energy between the carbon source and metal ions. These experimental results are very important for the further design of CD sensors for a large variety of analytes.