bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluation of the control effect of SAR inducers against citrus Huanglongbing applied by foliar spray, soil drench or trunk injection
Authors: Li Jinyun , Kolbasov Vladimir G. , Pang Zhiqian , Duan Shuo , Lee Donghwan , Huang Yixiao , Xu Jin , Teper Doron , Lamichhane Tirtha , Wang Nian 
Published: January 2021

AbstractHuanglongbing (HLB) or greening disease, associated with the bacterial pathogen Candidatus Liberibacter asiaticus (Las), is currently the most devastating citrus disease worldwide and no cure is available. Inducers of systemic acquired resistance (SAR) are effective and sustainable to combat various plant diseases. In this study, the SAR inducers acibenzolar-S-methyl (ASM), imidacloprid (IMI), 2,6-dichloroisonicotinic acid (INA), and salicylic acid (SA), applied individually by foliar spray, soil drench or trunk injection at various rates and frequencies, were evaluated for control of HLB in a 3-year field trial with mature Hamlin sweet orange trees in central Florida, USA in the 2016, 2017, and 2018 crop seasons. Six foliar sprays, six soil applications, and three trunk injections of ASM, IMI, INA, or SA per year were conducted with the untreated as a negative control. HLB disease severity, Las titers, pre-harvest fruit drop, yield and fruit quality were investigated for the treatments. By the end of the 2018 season, all trunk injection treatments at 0.25 g/tree and foliar sprays of INA or SA (but not ASM or IMI) at 0.5 g/tree significantly reduced disease severity, Las population, and fruit drop, and increased fruit yield; whereas all foliar spray treatments at 0.25 g/tree, trunk injection treatments at 0.125 g/tree, and soil drench treatments at 0.25 or 0.5 g/tree did not provide effective control of HLB. Additionally, all trunk injection treatments at 0.25 g/tree had shown a significant decrease in fruit drop and increase of fruit yield starting from 2016 after 1 year of applications, whereas foliar sprays of INA or SA at 0.5 g/tree exhibited similar effects at 2018 after 3 years of applications. None of the SAR inducer treatments had significant effect on fruit quality. Economic analysis suggested that the trunk injection treatments at 0.25 g/tree might produce financial benefits. Overall, this study presents useful information for management of citrus HLB with SAR inducers.
A combined geophysical and lithological study on eruptive history and Quaternary lacustrine stratigraphy of a maar in Leizhou Peninsula, China
Authors: Chen Cong , Zheng Zhuo , Zeng Li-Feng , Xiao Fan , Tian Li-Ping , Huang Kang-You 
Published: January 2021

AbstractAs the second common type of volcanic vent on Earth, maar-diatreme volcanoes and their post-eruptive lacustrine sediments are a main focus of volcanology, palaeolimnology, palaeoclimatology and palaeontology. A number of maar-type volcanoes have been found in Leizhou Peninsula, South China, but little is known about their eruption processes and detailed stratigraphy of the post-eruptive sediments. We present a combined geophysical and geological analysis to study the eruptive history and post-eruptive sediment stratigraphy of a large maar, the elliptical (1.8 × 3.0 km2) Jiudouyang (JDY) maar. The lacustrine stratigraphy revealed by drilling cores shows that the JDY maar lake has three major stages of evolution: (i) deep-lake sedimentary environment characterized by high autochthonous diatom productivity; (ii) shallow lake to swamp with very low water levels, characterized by a high total organic carbon (TOC) and abundant wood fragments; and, (iii) intermittent shallow lake and alluvial deposits composed of clay minerals and sand. The electrical resistivity tomography (ERT) values and lithological features are highly consistent, which clearly reveal the presence of ca. 50 m thick lacustrine sediments, directly underlain by a ca. 70 m thick basaltic lava rather than diatreme breccia in the crater. This infill sequence implies an alternation of eruption style from phreatomagmatic to Strombolian and/or lava flow, due to high magma flux and ascent rate of the Hainan Plume during the middle Pleistocene. The ERT data also reveal the initial phreatomagmatic crater floor at ca. 120 m depth. The initial crater had a large diameter/depth ratio (ca. 17), with an elongated shape (major axis to minor axis = 0.6), implying possible lateral vent migration during the eruption. A significant erosion under tropical weathering condition during the last few hundred thousand years, accounted for the large size of the maar crater. The study provides insights into the eruptive history and post-eruptive evolution of a large maar, as well as the spatial distribution of the lacustrine sediments.
A comprehensive review on bioactive peptides derived from milk and milk products of minor dairy species
Authors: Guha Snigdha , Sharma Heena , Deshwal Gaurav Kr , Rao Priyanka Singh 
Published: January 2021

Abstract
Milk from different species has been exploited for the isolation of various functional ingredients for decades. Irrespective of the source, milk is considered as a complete food, as it provides essential nutrients required by the human body. Proteins and their fractions are valuable sources of bioactive peptides that might exert a health beneficial role in the human body such as immune-modulation, antioxidant activity, ACE-inhibitory activity, anti-neoplastic, anti-microbial, etc. In milk, bioactive peptides may either be present in their natural form or released from their parental proteins due to enzymatic action. The increasing interest in bioactive peptides among researchers has lately augmented the exploration of minor dairy species such as sheep, goat, camel, mithun, mare, and donkey. Alternative to cow, milk from minor dairy species have also been proven to be healthier from infancy to older age owing to their higher digestibility and other nutritive components. Therefore, realizing the significance of milk from such species and incentivized interest towards the derivatization of bioactive peptides, the present review highlights the significant research achievements on bioactive peptides from milk and milk products of minor dairy species.

Graphical abstract
Effect of discontinuation of ticagrelor and switching-over to other P2Y12 agents in patients with acute coronary syndrome: a single-center real-world experience from India
Authors: Senguttuvan Nagendra Boopathy , Sankaran Ramesh , Rajeev Yashasvi , Thaiyal Devi , Mathew Angel , Dharsini K. , Marcelene Divya , Kalsingh Maria Jusler , Sahu Sujit Kumar , Sampath Aravind , Prem Kumar K. J. , Parthasarathy Harikrishnan , Louis Amal , Gnanaraj Anand , Reddy K. N. , Abraham K. A. 
Published: January 2021

Abstract
Background
Dual antiplatelet therapy is the current standard of care after acute coronary syndrome (ACS) and percutaneous coronary intervention (PCI). We intended to study the pattern of use of ticagrelor in patients with acute coronary syndrome undergoing PCI and the effect of switching over to other P2Y12 receptor inhibition on clinical outcomes.

Results
All patients aged > 18 years who had been admitted with acute coronary syndrome and had been provided ticagrelor as the second antiplatelet agent were included as study participants. The primary outcome of the study was the composite outcome of death, recurrent myocardial infarctions, re-intervention, and major bleeding.
We studied 321 patients (54 female patients, 16.82%). The mean age of the patients was 56.65 ± 11.01 years. Ticagrelor was stopped in 76.7% on follow-up. It was stopped in 6.3%, 13.5%, 13.1%, 21.9%, and 45.1% of patients during the first month but after discharge, between first and third months, between 3 and 6 months, between 6 and 12 months, and after 12 months, respectively. In the majority of patients, ticagrelor was replaced by clopidogrel (97.9%). It was stopped according to the physician’s discretion in 79.3% of patients, whereas it was the cost of the drug that made the patient to get swapped to another agent in 18.6%. No difference in the primary composite outcome was observed between the groups where ticagrelor was continued post 12 months and ticagrelor was continued and ticagrelor was switched-over to another agent. Similarly, no difference in death, recurrent myocardial infarctions, re-interventions, or major bleeding manifestations was observed between the two groups.

Conclusion
In patients with acute coronary syndrome who undergo PCI, we observed that early discontinuation of ticagrelor and switching over to other P2Y12 inhibitors after discharge did not affect clinical outcomes.
The added value of chemical shift imaging in evaluation of bone marrow changes in sickle cell disease
Authors: Allam Mohammad Fouad Abdel-Baki , Samra Manal Fayez Abu , Rahman Al Shaimaa Mahfouz Abdel 
Published: January 2021

Abstract
Background
The aim of this study was to assess the added value of chemical shift imaging when used with routine MRI study in evaluation of bone marrow changes in SCD. Forty-two patients with SCD and bone pain were included in the study; they underwent CSI and routine MRI study on the symptomatic anatomic part of the skeleton.

Results
Four patterns of diffuse bone marrow changes were recognized; they varied from persistent red marrow to diffuse hypointense patterns with abnormal signal loss percentage on CSI that suggest presence of iron overload (n = 28, 66.6%). Serum ferritin level was increasing in accordance to the degree of signal changes found on CSI with significant high negative correlation between the percentage of signal loss on CSI obtained from IP-OP/IP formula and serum ferritin level. In focal marrow lesions, all T1 hyperintense lesions demonstrated corresponding hyperintensity on IP and OP; the detection frequency on CSI was relatively higher on OP compared with IP images.

Conclusion
CSI has high diagnostic performance in detecting diffuse marrow changes and development of iron overload in SCD. In SCD-related focal marrow lesions, CSI could have a complementary role in detection of T1 hyperintensity and lesion conspicuity.
Chemical profiling of selected Ayurveda formulations recommended for COVID-19
Authors: C. T. Sulaiman , M. Deepak , P. R. Ramesh , K. Mahesh , E. M. Anandan , Balachandran Indira 
Published: January 2021

Abstract
Background
The novel coronavirus disease 2019 (COVID-19), caused by the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), is the global health concern since December 2019. It has become a big challenge for the researchers to find a solution for this newly evolved pandemic. In Ayurveda point of view, COVID-19 is a Janapadodhwamsa vikara (epidemic disease), a situation where the environment—air, water, land, and seasons—is vitiated, causing a simultaneous manifestation of a disease among large populations. The aim of this study is to identify the active compounds of selected Ayurveda medicines recommended for COVID-19.

Results
The selected preparations are traditionally recommended for the management of various kinds of fever including the infectious ones and to enhance the immunity. HPTLC analysis of the same showed presence of many active molecules like umbelliferone, scopoletin, caffeic acid, ferulic acid, gallic acid, piperine, curcumin, berberine, and palmatine.

Conclusion
The study provided valuable scientific data regarding the active ingredients of the selected medicines with proven therapeutic potentials like anti-viral, immunomodulatory, and anti-inflammatory activities.
Pharmacognostical characterization and formulation of herbal-based low-cost mosquito repellents from Elettaria cardamomum (Linn.) seed by using natural binder
Authors: Das Rajat , Pal Prosanta , Bhutia Sonam 
Published: January 2021

Abstract
Background
In the present scenario, the markets are flooded with chemical-based mosquito repellent products that are proven to be toxic to the human life especially to the children and quite expensive in term of cost. This present investigation was attempted to evaluate the complete pharmacognostical characteristics of Elettaria cardamomum (Linn.) seed and to formulate eco-friendly and cost-effective herbal mosquito repellents of the seed powder by using a natural binder.

Results
The results of microscopic study of seed confirmed the presence of perisperm, embryo, endosperm and testa (Figs. 4, 5 and 6). The extractive values, moisture contents and ash values were also analysed and found to be alcoholic extract, 4.88% w/w; water extract, 40% w/w; moisture content, 8.6%; total ash, 5% w/w; acid insoluble ash, 1.5% w/w; and water soluble ash, 3.5% w/w (summarized in Tables 1, 2 and 3). From the mosquito repellency test (Table 6), the combination of cardamom seed cake was done with cardamom powder 3/2 + 1/4 spoons, wood powder 3 spoons and chilli powder 1/4 spoon which showed low residual percentage of 15.05%, no irritation and an average burning time of 65 min. The formulated herbal-based mosquito repellents were found to be more effective without any side effects and found less cost too.

Conclusion
All the ingredients used in the formulation were herbal based and has no side effect on human health. The cost of the cake was Rs. 11.332/- and it can be considered as good mosquito repellent cakes because of its consistent burning ability with no irritating smoke and low residual percentage and high mosquito repellent ability. By utilizing this concept, the researchers can develop herbal, eco-friendly and cost-effective mosquito repellents in future.
Anticancer studies on methanolic extract of aerial parts of Uraria Picta (Jacq.) DC
Authors: Chhipa Abu Sufiyan , Baksi Ruma , Nivsarkar Manish 
Published: January 2021

Abstract
Background
Uraria picta (Jacq.) DC is a traditional Indian herb used in the Ayurvedic system of medicine. It is an ingredient of the popular Ayurvedic formulation “Dasamoola.” It is also traditionally used as an anti-inflammatory herb in Ayurveda. The plant also has excellent antioxidant potential. Different parts of Uraria picta are useful and have diverse medicinal properties. The present investigation was done to evaluate the anticancer activity of methanolic extract of aerial parts of Uraria picta (Jacq.).

Results
Preliminary phytochemical screening of MEUP confirmed the presence of flavonoids, phenolics, glycosides, and tannins. Total phenolic and flavonoid contents in MEUP were found to be 14.6 gram equivalent of gallic acid and 0.735 gram equivalent of catechin, respectively. IC50 values of cisplatin and MEUP were found to be 8.75 μg/ml and 436.92 μg/ml, respectively. Induction of apoptosis was evident from DAPI staining showing a dose-dependent increase in apoptosis. Also, a reduction in intracellular ROS was evident from the DCFH-da assay, where a dose-dependent decrease in intracellular ROS (fluorescence) was observed. Significant reduction (P < 0.05) in tumor volume was observed in mice receiving cisplatin and MEUP at both dose levels.

Conclusion
The methanolic extract of aerial parts of Uraria picta (Jacq.) DC is effective in inhibiting tumor growth and has significant anticancer property.
Evaluation of the anti-bacterial activity of methanolic extract of Chlorella vulgaris Beyerinck [Beijerinck] with special reference to antioxidant modulation
Authors: Pradhan Biswajita , Patra Srimanta , Dash Soumya Ranjan , Nayak Rabindra , Behera Chhandashree , Jena Mrutyunjay 
Published: January 2021

Abstract
Background
The natural antioxidants from Chlorella have potent therapeutic implication in several diseases. However, the anti-bacterial activity and their molecular mode of action have not been investigated yet. The present study focussed on the assessment of antioxidant potential as well as free radical scavenging activity such as DPPH, hydroxyl radical, hydrogen peroxide, and superoxide anion radical assay of Chlorella vulgaris Beyerinck [Beijerinck] (BUACC25) isolated from marine habitat. Furthermore, the anti-bacterial activity and their molecular mode of action have been evaluated.

Results
In the present study, the preliminary phytochemical screening of methanolic algal extract revealed the presence of alkaloids, glycosides, proteins, terpenoids, saponins, coumarin, phenols, and tannins, which was confirmed by in an UV-visible and FT-IR spectroscopy, indicated the distinct spectral peaks. The methanolic algal extract was found to be rich in phenolic content (45 ± 0.06 mg GAE g−1) and flavonoid content (470 ± 0.25 mg of RUE g−1). Furthermore, the methanolic extract was revealed potent antioxidant scavenging activity to scavenge various free radicals with minimum IC50 values of DPPH, hydroxyl, H2O2, superoxide 2.82 ± 0.30, 2.30 ± 0.25, 3.24 ± 0.32, and 3.15 ± 0.02 μg ml−1 respectively. Furthermore, the methanolic extract of C. vulgaris exhibited potent anti-bacterial activity which was evident with the reduction in cfu × 107/ml and % of cell viability. Mechanistically, reduction of SOD, CAT, and GSH activity provoked ROS-mediated cell death after drug treatment. Moreover, in combination with norfloxacin and ciprofloxacin, methanolic extract of C. vulgaris demonstrated enhanced anti-bacterial activity with an evident reduction in cfu/ml and % of cell viability.

Conclusion
This study advocates that C. vulgaris (BUACC25) has promising antioxidant activity owing to the presence of phenolic and flavonoids evidenced by scavenging of DPPH, hydroxyl, H2O2, and superoxide radicals. In addition to this, it sustained anti-microbial activity against E. coli through modulation of SOD, CAT, and GSH. This study carved a path for uncovering a better therapeutic agent against disease-causing bacterial pathogens.
Screening and Preventative Strategies for Patients at High Risk for Breast Cancer
Authors: Ter-Minassian Monica , Schaeffer Marcy L. , Jefferson Celeena R. , Shapiro Stacey C. , Suwannarat Pim , Visvanathan Kala 
Published: January 2021

PURPOSE: Current US guidelines recommend more intensive breast cancer screening and preventive strategies for patients at more than 20% lifetime risk for breast and ovarian cancer (high risk for breast and ovarian cancer [HRBOC]). Guidelines recommend that yearly mammograms alternating with magnetic resonance imaging (MRI) screening should be considered as early as 30 years old. Furthermore, BRCA mutation carriers should consider bilateral mastectomy and bilateral oophorectomy after age 35. It was unclear what the uptake of screening and risk-reducing strategies were for patients who were cancer-free and cancer survivors seen by Kaiser Permanente Mid-Atlantic States (KPMAS) Genetics. METHODS: We retrospectively studied female patients (members of KPMAS between 2005 and 2016) diagnosed as HRBOC and/or tested for breast cancer-related mutations by KPMAS Genetics during 2013-2016. We identified cancer diagnoses, mammogram and breast MRI screening, mastectomies, and oophorectomies that occurred before and after the Genetics visit during the study period. RESULTS: Our cohort included 813 women with a HRBOC diagnosis, with a median 51 years of age at diagnosis, 45% White, 38% Black, and 15% other ethnicity. Most cancers occurred prior to the Genetics visit: 513/527 breast cancer diagnoses and 55/57 ovarian cancer diagnoses. Fewer than five prophylactic mastectomies and 89 prophylactic oophorectomies were identified. Among 228 patients who were 30-75 years old, breast cancer–free at the time of HRBOC diagnosis, and members for over 6 months, 190 (83%) had at least one screening test (mammogram or MRI) after the consultation with Genetic, but 79% never had an MRI before or after the consultation. CONCLUSION: Our findings suggest that earlier detection of patients with HRBOC and closer monitoring is needed.
Disparities in Characteristics, Access to Care, and Oncologic Outcomes in Young-Onset Colorectal Cancer at a Safety-Net Hospital
Authors: Fangman Benjamin D. , Goksu Suleyman Y. , Chowattukunnel Nivan , Beg Muhammad S. , Sanford Nina N. , Sanjeevaiah Aravind , Cox John , Folkert Michael R. , Aguilera Todd A. , Mathews Joselin , Pogacnik Javier Salgado , Khatri Gaurav , Olson Craig , Polanco Patricio M. , Verma Udit , Hsiehchen David , Jones Amy , Kainthla Radhika , Kazmi Syed M. 
Published: January 2021

PURPOSE: Young-onset colorectal cancer is an emerging cause of significant morbidity and mortality globally. Despite this, limited data exist regarding clinical characteristics and outcomes, particularly in safety-net populations where access to care is limited. We aimed to study disparities in clinical characteristics and outcomes in patients with young-onset colorectal cancer in the safety-net setting. METHODS: We performed a retrospective review of patients < 50 years old diagnosed and/or treated for colorectal cancer between 2001 and 2017 at a safety-net hospital. Kaplan-Meier and Cox regression models were constructed to compare overall survival (OS), progression-free survival (PFS), and relapse-free survival (RFS) by race and ethnicity, stratifying for relevant clinical and pathologic factors. RESULTS: A total of 395 young-onset patients diagnosed at a safety-net hospital were identified and 270 were included in the analysis (49.6% Hispanic, 25.9% non-Hispanic Black, 20.0% non-Hispanic White, and 4.4% other). Non-Hispanic White race was independently associated with worse OS (hazzard ratio [HR], 0.53; 95% CI, 0.29 to 0.97), as were lack of insurance, higher clinical stage, and mismatch repair proficiency. There was no significant difference seen in PFS or RFS between racial and ethnic groups. CONCLUSION: Non-Hispanic White race or ethnicity was found to be independently associated with worse OS in a safety-net population of patients with young-onset colorectal cancer. Other independent predictors of worse OS include higher stage, lack of insurance, and mismatch repair proficiency.
Latency of mechanically stimulated escape responses in the Pacific spiny dogfish, Squalus suckleyi
Authors: Schakmann Mathias , Becker Victoria , Søgaard Mathias , Johansen Jacob L. , Steffensen John F. , Domenici Paolo 
Published: January 2021

ABSTRACT
Fast escape responses to a predator threat are fundamental to the survival of mobile marine organisms. However, elasmobranchs are often underrepresented in such studies. Here, we measured the escape latency (time interval between the stimulus and first visible reaction) of mechanically induced escape responses in the Pacific spiny dogfish, Squalus suckleyi, and in two teleosts from the same region, the great sculpin, Myoxocephalus polyacanthocephalus, and the pile perch, Rhacochilus vacca. We found that the dogfish had a longer minimum latency (66.7 ms) compared with that for the great sculpin (20.8 ms) and pile perch (16.7 ms). Furthermore, the dogfish had a longer latency than that of 48 different teleosts identified from 35 different studies. We suggest such long latencies in dogfish may be due to the absence of Mauthner cells, the giant neurons that control fast escape responses in fishes.