bluuebunny/crossref_metadata_2025_split huggingface dataset:
Image-based deep learning reveals the responses of human motor neurons to stress and ALS
Authors: Verzat Colombine , Harley Jasmine , Patani Rickie , Luisier Raphaëlle 
Published: April 2021

SUMMARYAlthough morphological attributes of cells and their substructures are recognized readouts of physiological or pathophysiological states, these have been relatively understudied in amyotrophic lateral sclerosis (ALS) research. In this study we integrate multichannel fluorescence high-content microscopy data with deep-learning imaging methods to reveal - directly from unsegmented images - novel neurite-associated morphological perturbations associated with (ALS-causing) VCP-mutant human motor neurons (MNs). Surprisingly, we reveal that previously unrecognized disease-relevant information is withheld in broadly used and often considered ‘generic’ biological markers of nuclei (DAPI) and neurons (βIII-tubulin). Additionally, we identify changes within the information content of ALS-related RNA binding protein (RBP) immunofluorescence imaging that is captured in VCP-mutant MN cultures. Furthermore, by analyzing MN cultures exposed to different extrinsic stressors, we show that heat stress recapitulates key aspects of ALS. Our study therefore reveals disease-relevant information contained in a range of both generic and more specific fluorescent markers, and establishes the use of image-based deep learning methods for rapid, automated and unbiased testing of biological hypotheses.
PKR-mediated Stress Response Enhances Dengue and Zika Virus Replication
Authors: Ricciardi-Jorge Taissa , Lummertz da Rocha Edroaldo , Gonzalez-Kozlova Edgar , Rodrigues-Luiz Gabriela Flavia , Ferguson Brian J , Sweeney Trevor , Irigoyen Nerea , Mansur Daniel Santos 
Published: April 2021

ABSTRACTThe mechanisms by which flaviviruses use non-canonical translation to support their replication in host cells are largely unknown. Here we investigated how the integrated stress response (ISR), which promotes translational arrest by eIF2ɑ phosphorylation (p‒eIF2ɑ), regulates flavivirus replication. During Dengue virus (DENV) and Zika virus (ZIKV) infection, eIF2ɑ phosphorylation peaked at 24 hours post infection and was dependent on PKR but not type I interferon. The ISR is activated downstream of p-eIF2α during infection with either virus, but translation arrest only occurred following DENV4 infection. Despite this difference, both DENV4 and ZIKV replication was impaired in cells lacking PKR, independent of IFN-I/NF-kB signalling or cell viability. By using a ZIKV 5′ UTR reporter system as a model, we found that this region of the genome is sufficient to promote an enhancement of viral mRNA translation in the presence of an active ISR. Together we provide evidence that flaviviruses escape ISR translational arrest and co-opt this response to increase viral replication.
Calcium waves facilitate and coordinate the contraction of endfeet actin stress fibers inDrosophilainterommatidial cells
Authors: Ready Donald F. , Chang Henry C. 
Published: April 2021

AbstractActomyosin contraction shapes theDrosophilaeye’s panoramic view. The convex curvature of the retinal epithelium, organized in ∼800 close-packed ommatidia, depends upon a fourfold condensation of the retinal floor mediated by contraction of actin stress fibers in the endfeet of interommatidialcells (IOCs). How these tensile forces are coordinated is not known. Here, we discover a novel phenomenon: Ca2+waves regularly propagate across the IOC network in pupal and adult eyes. Genetic evidence demonstrates that IOC Ca2+waves are independent of phototransduction, but require inositol 1,4,5-triphosphate receptor (IP3R), suggesting these waves are mediated by Ca2+releases from ER stores. Removal ofIP3Rdisrupts stress fibers in IOC endfeet and increases the basal retinal surface by ∼40%, linking IOC waves to facilitating stress fiber contraction and floor morphogenesis. Further,IP3Rloss disrupts the organization of a collagen IV network underneath the IOC endfeet, implicating ECM and its interaction with stress fibers in eye morphogenesis. We propose that coordinated Ca2+spikes in IOC waves promote stress fiber contractions, ensuring an organized application of the planar tensile forces that condense the retinal floor.Summary StatementCa2+waves have an important role in generating tensile forces to shape theDrosophilaeye’s convex curvature. Coordinated Ca2+spikes facilitate actomyosin contractions at the basal endfeet of interommatidial cells.
Widespread false gene gains caused by duplication errors in genome assemblies
Authors: Ko Byung June , Lee Chul , Kim Juwan , Rhie Arang , Yoo DongAhn , Howe Kerstin , Wood Jonathan , Cho Seoae , Brown Samara , Formenti Giulio , Jarvis Erich D. , Kim Heebal 
Published: April 2021

AbstractFalse duplications in genome assemblies lead to false biological conclusions. We quantified false duplications in previous genome assemblies and their new counterparts of the same species (platypus, zebra finch, Anna’s hummingbird) generated by the Vertebrate Genomes Project (VGP). Whole genome alignments revealed that 4 to 16% of the sequences were falsely duplicated in the previous assemblies, impacting hundreds to thousands of genes. These led to overestimated gene family expansions. The main source of the false duplications was heterotype duplications, where the haplotype sequences were more divergent than other parts of the genome leading the assembly algorithms to classify them as separate genes or genomic regions. A minor source was sequencing errors. Although present in a smaller proportion, we observed false duplications remaining in the VGP assemblies that can be identified and purged. This study highlights the need for more advanced assembly methods that better separates haplotypes and sequence errors, and the need for cautious analyses on gene gains.
Physicochemical characteristics and mineral status of honey from different agro-climatic zones of Himachal Pradesh, India
Authors: Thakur Meena , Gupta Neha , Sharma Harish Kumar , Devi Sunita 
Published: April 2021

PurposeThe purpose of this study is to assess the quality of honey from different agro-climatic zones of Himachal Pradesh in terms of physicochemical characteristics and mineral status.Design/methodology/approachThree honey-producing locations were selected within each agro-climatic zone, honey sampled from four separate apiaries within each location and analyzed for physicochemical characteristics and mineral status using standard methodologies. The data were analyzed using one-way analysis of variance with one-way classification, after appropriate transformation through online OP-STAT software and MS Excel. The correlation coefficient (r) was also calculated. Principal component analysis was done using XL-STAT software.FindingsThe honey of Zone 4 had highest fructose (36.62%), F:G ratio (1.55), acidity (46.07 meq/kg), vitamin C (25.04 mg/100 g) and diastase (19.22 DN), whereas the pollen density (76,666.67 pollen grains per 10 g), pH (5.94), sucrose (6.94%), hydroxy methyl furfuraldehyde (70.20 mg/kg), amino acid (103.83 mg/100 g), phenols (77.39 mg/100 g), Ca (81.04 mg/kg) and K (354.17 mg/kg) were highest for Zone 2. Highest electrical conductivity (0.24 mS/cm), moisture (16.50 %), glucose (34.20%) and P content (62.93 mg/kg) were recorded for Zone 1. Correlation studies indicated a significant positive correlation between pH and EC; EC and moisture; colour and pollen density. Examining the graphical distribution of the honey samples, a natural separation between honeys of four different agro-climatic zones was obtained.Originality/valueThe impact of geographical/agro-climatic variations in physicochemical characteristics of honey has not been worked out under the present scenario in Himachal Pradesh.
Inclusion of Indigenous workers in workplace mental health
Authors: O'Loughlin Robyn A. , Kristman Vicki L. , Gilbeau Audrey 
Published: April 2021

PurposeThis paper highlights inclusion issues Indigenous people experience maintaining their mental health in the workplace.Design/methodology/approachUsing a grounded theoretical approach, five sharing circles were conducted with the Nokiiwin Tribal Council's community members to better understand inclusivity issues related to workplace mental health.FindingsFive themes emerged from the data related to enhancing inclusivity and workplace mental health for Indigenous workers: (1) connecting with individuals who understand and respect Indigenous culture; (2) respecting Indigenous traditions; (3) hearing about positive experiences; (4) developing trusting relationships and (5) exclusion is beyond the workplace.Research limitations/implicationsThe next step is to finalize development of the Wiiji app and evaluate the effectiveness of the app in helping Indigenous workers feel included at work and to improve workplace mental health. If effective, the Indigenous-developed e-mental health app will be promoted and its benefits for helping Indigenous workers feel included at work and also for providing accessible mental health resources, will be known. In the future, other Indigenous groups may be potentially interested in adopting a similar application in their workplace(s).Originality/valueThere is very little known about inclusivity issues related to Indigenous workers' maintaining their mental health. This paper identifies major issues influencing the exclusion and inclusion of Indigenous workers.
Big data analytics capabilities and organizational performance: the mediating effect of dual innovations
Authors: Su Xiaofeng , Zeng Weipeng , Zheng Manhua , Jiang Xiaoli , Lin Wenhe , Xu Anxin 
Published: April 2021

PurposeFollowing the rapid expansion of data volume, velocity and variety, techniques and technologies, big data analytics have achieved substantial development and a surge of companies make investments in big data. Academics and practitioners have been considering the mechanism through which big data analytics capabilities can transform into their improved organizational performance. This paper aims to examine how big data analytics capabilities influence organizational performance through the mediating role of dual innovations.Design/methodology/approachDrawing on the resource-based view and recent literature on big data analytics, this paper aims to examine the direct effects of big data analytics capabilities (BDAC) on organizational performance, as well as the mediating role of dual innovations on the relationship between (BDAC) and organizational performance. The study extends existing research by making a distinction of BDACs' effect on their outcomes and proposing that BDACs help organizations to generate insights that can help strengthen their dual innovations, which in turn have a positive impact on organizational performance. To test our proposed research model, this study conducts empirical analysis based on questionnaire-base survey data collected from 309 respondents working in Chinese manufacturing firms.FindingsThe results support the proposed hypotheses regarding the direct and indirect effect that BDACs have on organizational performance. Specifically, this paper finds that dual innovations positively mediate BDACs' effect on organizational performance.Originality/valueThe conclusions on the relationship between big data analytics capabilities and organizational performance in previous research are controversial due to lack of theoretical foundation and empirical testing. This study resolves the issue by provides empirical analysis, which makes the research conclusions more scientific and credible. In addition, previous literature mainly focused on BDACs' direct impact on organizational performance without making a distinction of BDAC's three dimensions. This study contributes to the literature by thoroughly introducing the notions of BDAC's three core constituents and fully analyzing their relationships with organizational performance. What's more, empirical research on the mechanism of big data analytics' influence on organizational performance is still at a rudimentary stage. The authors address this critical gap by exploring the mediation of dual innovations in the relationship through survey-based research. The research conclusions of this paper provide new perspective for understanding the impact of big data analytics capabilities on organizational performance, and enrich the theoretical research connotation of big data analysis capabilities and dual innovation behavior.
Performance appraisal justice and affective commitment: examining the moderating role of age and gender
Authors: Rana Shikha , Singh Shalini 
Published: April 2021

PurposePerformance appraisal (PA) is one of the most indispensable human resource management practices as many critical decisions regarding employees’ performance are heavily based on the PA results as they are responsible for various attitude-related outcomes. This study aims to investigate the relationship between PA justice and affective commitment (AC) of employees in the Indian banking sector, and to examine the moderating effects of age and gender on this relationship.Design/methodology/approachA total of 298 employees from the banking sector were randomly selected to examine the relationship between PA justice and AC along with the moderating role of age and gender on this relationship through structural equation modelling using AMOS 21.FindingsThe findings of the study supported hypothesized relationships as PA justice significantly predicted the AC of bank employees in India. The moderating effects of age and gender on the relationship of PA justice and AC are found to be high for older employees and female employees, respectively.Research limitations/implicationsAs the present study was cross-sectional, so any inferences regarding causality are limited. Theoretical and managerial implications have been discussed in the context of banking sector.Originality/valueThe paper contributes new insights to the existing literature by examining the moderating effects of age and gender on the relationship of PA justice and AC in the context of Indian banking sector.
Reconciling conflicting predictions about transience and persistence of management concepts in management fashion theory and new institutionalism
Authors: Aksom Herman 
Published: April 2021

PurposeAlthough drawing from neoinstitutional theoretical apparatus and ontology, management fashion theory is understood as a theory that explains the transitory nature of popular ideas and practices while institutional theory explains their stabilization, persistence and further institutionalization. In a nutshell, it seems that being opposed to each other, these two theories describe and predict different, incommensurable diffusion trajectories and organizational behaviour patterns. The purpose of this paper is to unify these two competing perspectives.Design/methodology/approachThis paper makes an attempt toward further unification of management fashion theory with new institutionalism by offering an alternative understanding and conceptualization of institutional change and deinstitutionalization and by distinguishing emerging concepts from already popular fashions.FindingsMost emerging concepts never achieve popularity and disappear while few of them achieve massive media attention and diffuse widely becoming new management fashions. Once these concepts have achieved a wide popularity institutional forces would favor them and lead to further institutionalization. Institutional change is understood not as a deinstitutionalization of existing management fashion in terms of erosion, discontinuity or disappearance but as a decline in its media coverage while media attention focuses on new fashionable concept. The former management fashion gets institutionalized, institutional change occurs in terms of shifting attention toward new fashion and diffusion and institutionalization cycle restarts. Institutional prediction of isomorphism and institutionalization as irreversible tendencies thus can be unified with MF prediction about the bell-shaped curves in fashions’ popularity. Therefore, postulates and predictions of management fashion theory can be derived from new institutionalism and vice versa.Practical implicationsThe paper aims to cover, generalize and explain different trajectories of various management and organizational concepts, deducing theoretical propositions from both institutional theory and management fashion theory. Theoretical and methodological ideas offered in this paper can be helpful in future research on management fashions and diffusion. Studies on the evolution of management concept can benefit from proposed categorization and causal relationships between different stages of the life cycle.Originality/valueUnifying seemingly conflicting and disparate perspectives and views allows making organization theory more coherent in terms of both explanatory power and ontological commensurability. Following other mature sciences, we share the same notion of progress, namely, the aim of achieving unification and demonstrating that different organizational theories still describe the same reality.
Shopping without the fuss: the effect of curation type in clothing subscription adoption on cognitive dissonance and consumer responses
Authors: Johnson Olivia , Seifert Christin , Lee Angie 
Published: April 2021

PurposeTo address the volatile nature of the retail industry, retailers have adopted clothing subscription services (CSS) to meet the demanding needs of consumers. This study provides insight into different types of CSS, as well as a process by which behavioural intentions are influenced by CSS type through cognitive dissonance (wisdom of purchase and emotional dissonance) and attitude towards the CSS.Design/methodology/approachAn experimental design manipulating the CSS type (full/partial/none) was conducted among 358 US consumers to test the proposed hypotheses.FindingsHayes PROCESS macro model results demonstrated that consumers did not experience more cognitive dissonance towards a partially, fully curated or non-curated CSS. However, a significant interaction effect further uncovered that consumers with high aesthetic perception experience more negative wisdom of purchase towards a fully compared to a partially curated CSS, thereby impacting attitude and behavioural intention towards CSS.Practical implicationsDue to today's rapidly evolving retail industry, retailers endeavouring to engage in this business model should come up with strategies to turn a visitor into a subscriber and decrease hesitation in novice consumers. Moreover, retailers should ascertain consumers’ level of aesthetic perception as it plays an important role in CSS adoption.Originality/valueWe introduced a unique operationalization of CSS types by differentiating between fully, partially and non-curated subscriptions, which are commonly employed in the subscription-box marketplace. The previous literature rarely makes distinctions between these types, although our findings show that consumers perceive them differently.
Jealousy due to social media? A systematic literature review and framework of social media-induced jealousy
Authors: Tandon Anushree , Dhir Amandeep , Mäntymäki Matti 
Published: April 2021

PurposeThe association between social media and jealousy is an aspect of the dark side of social media that has garnered significant attention in the past decade. However, the understanding of this association is fragmented and needs to be assimilated to provide scholars with an overview of the current boundaries of knowledge in this area. This systematic literature review (SLR) aims to fulfill this need.Design/methodology/approachThe authors undertake an SLR to assimilate the current knowledge regarding the association between social media and jealousy, and they examine the phenomenon of social media-induced jealousy (SoMJ). Forty-five empirical studies are curated and analyzed using stringent protocols to elucidate the existing research profile and thematic research areas.FindingsThe research themes emerging from the SLR are (1) the need for a theoretical and methodological grounding of the concept, (2) the sociodemographic differences in SoMJ experiences, (3) the antecedents of SoMJ (individual, partner, rival and platform affordances) and (4) the positive and negative consequences of SoMJ. Conceptual and methodological improvements are needed to undertake a temporal and cross-cultural investigation of factors that may affect SoMJ and acceptable thresholds for social media behavior across different user cohorts. This study also identifies the need to expand current research boundaries by developing new methodologies and focusing on under-investigated variables.Originality/valueThe study may assist in the development of practical measures to raise awareness about the adverse consequences of SoMJ, such as intimate partner violence and cyberstalking.
Sensegiving in organizations via the use of business analytics
Authors: Namvar Morteza , Intezari Ali , Im Ghiyoung 
Published: April 2021

PurposeBusiness analytics (BA) has been a breakthrough technological development in recent years. Although scholars have suggested several solutions in using these technologies to facilitate decision-making, there are as of yet limited studies on how analysts, in practice, improve decision makers' understanding of business environments. This study uses sensemaking theory and proposes a model of how data analysts generate analytical outcomes to improve decision makers' understanding of the business environment.Design/methodology/approachThis study employs an interpretive field study with thematic analysis. The authors conducted 32 interviews with data analysts and consultants in Australia and New Zealand. The authors then applied thematic analysis to the collected data.FindingsThe thematic analysis discovered four main sensegiving activities, including data integration, trustworthiness analysis, appropriateness analysis and alternative selection. The proposed model demonstrates how these activities support the properties of sensemaking and result in improved decision-making.Research limitations/implicationsThis study provides strong empirical evidence for the theory development and practice of sensemaking. It brings together two distinct fields – sensemaking and business analytics – and demonstrates how the approaches advocated by these two fields could improve analytics applications. The findings also propose theoretical implications for information system development (ISD).Practical implicationsThis study demonstrates how data analysts could use analytical tools and social mechanisms to improve decision makers' understanding of the business environment.Originality/valueThis study is the first known empirical study to conceptualize the theory of sensemaking in the context of BA and propose a model for analytical sensegiving in organizations.