bluuebunny/crossref_metadata_2025_split huggingface dataset:
Src-mediated tyrosine phosphorylation of PRC1 and kinastrin/SKAP on the mitotic spindle
Authors: Morii Mariko , Kubota Sho , Hasegawa Chizu , Takeda Yumi , Kometani Shiori , Enomoto Kyoko , Suzuki Takayuki , Yanase Sayuri , Sato Rika , Akatsu Aki , Hirata Kensuke , Honda Takuya , Kuga Takahisa , Tomonaga Takeshi , Nakayama Yuji , Yamaguchi Noritaka , Yamaguchi Naoto 
Published: January 2021

AbstractSrc-family tyrosine kinases (SFKs) play important roles in a number of signal transduction events during mitosis, such as spindle formation. A relationship has been reported between SFKs and the mitotic spindle; however, the underlying mechanisms remain unclear. We herein demonstrated that SFKs accumulated in the centrosome region at the onset of mitosis. Centrosomal Fyn increased in the G2 phase in a microtubule polymerization-dependent manner. A mass spectrometry analysis using mitotic spindle preparations was performed to identify tyrosine-phosphorylated substrates. Protein regulator of cytokinesis 1 (PRC1) and kinastrin/small kinetochore-associated protein (kinastrin/SKAP) were identified as SFK substrates. SFKs mainly phosphorylated PRC1 at Tyr-464 and kinastrin at Tyr-87. Although wild-type PRC1 is associated with microtubules, phosphomimetic PRC1 impaired the ability to bind microtubules. Phosphomimetic kinastrin at Tyr-87 also impaired binding with microtubules. Collectively, these results suggest that tyrosine phosphorylation of PRC1 and kinastrin plays a role in their delocalization from microtubules during mitosis.
Meta-analysis of host transcriptional responses to SARS-CoV-2 infection reveals their manifestation in human tumors
Authors: Chen Fengju , Zhang Yiqun , Sucgang Richard , Ramani Sasirekha , Corry David , Kheradmand Farrah , Creighton Chad J. 
Published: January 2021

AbstractA deeper understanding of the molecular biology of SARS-CoV-2 infection, including the host response to the virus, is urgently needed. Commonalities exist between the host immune response to viral infections and cancer. Here, we defined transcriptional signatures of SARS-CoV-2 infection involving hundreds of genes common across lung adenocarcinoma cell lines (A549, Calu-3) and normal human bronchial epithelial cells (NHBE), with additional signatures being specific to one or both adenocarcinoma lines. Cross-examining eight transcriptomic databases, we found that host transcriptional responses of lung adenocarcinoma cells to SARS-CoV-2 infection shared broad similarities with host responses to multiple viruses across different model systems and patient samples. Furthermore, these SARS-CoV-2 transcriptional signatures were manifested within specific subsets of human cancer, involving ~ 20% of cases across a wide range of histopathological types. These cancer subsets show immune cell infiltration and inflammation and involve pathways linked to the SARS-CoV-2 response, such as immune checkpoint, IL-6, type II interferon signaling, and NF-κB. The cell line data represented immune responses activated specifically within the cancer cells of the tumor. Common genes and pathways implicated as part of the viral host response point to therapeutic strategies that may apply to both SARS-CoV-2 and cancer.
MicroRNA‑331 inhibits isoproterenol‑induced expression of profibrotic genes in cardiac myofibroblasts via the TGFβ/smad3 signaling pathway
Authors: Yousefi Fatemeh , Soltani Bahram M. , Rabbani Shahram 
Published: January 2021

AbstractCardiac fibrosis in the failing heart is modulated by activated myofibroblasts, and is a pathology marked by their deposition of extracellular matrix proteins. The TGFβ signaling pathway is important in stimulating fibrosis and therefore seems an attractive new target for anti-fibrotic therapy. The relationship between ncRNAs and TGFβ signaling pathway has been extensively studied. Here, we have provided several lines of evidence to prove that the fibrosis process could be regulated by miR-331 through targeting TGFβ signaling. First, bioinformatics analysis and dual luciferase assay validated a direct interaction between the miR-331 and TGFβ-R1 3′UTR sequence which results in the downregulation of TGFβ signaling pathway. Second, miR-331 expression was inversely related to the expression of a number of genes which are involved in extracellular matrix (ECM) production and deposition processes, both in the in vivo and in vitro fibrosis models. Third, in cultured mouse and human cardiac myofibroblasts (CMyoFbs) under ISO treatment, overexpression of miR-331 decreased the expression level of fibrosis-related genes. Consistently, western blot analysis confirmed that miR-331 overexpression ended in both Smad3 and Col1A1 protein level reduction in mouse cardiac myofibroblasts. Finally, flow cytometry analysis, cyclin D1 and D2 gene expression analysis, and wound-healing assay confirmed the inhibitory effect of miR-331 against cell proliferation and migration in ISO-treated cardiac myofibroblasts. Taken together, accumulative results showed that miR-331 reduced the level of fibrosis-related proteins in cardiac myofibroblasts culture via regulating TGFβ signaling pathway.
Detection of volcanic unrest onset in La Palma, Canary Islands, evolution and implications
Authors: Fernández José , Escayo Joaquín , Hu Zhongbo , Camacho Antonio G. , Samsonov Sergey V. , Prieto Juan F. , Tiampo Kristy F. , Palano Mimmo , Mallorquí Jordi J. , Ancochea Eumenio 
Published: January 2021

AbstractLa Palma island is one of the highest potential risks in the volcanic archipelago of the Canaries and therefore it is important to carry out an in-depth study to define its state of unrest. This has been accomplished through the use of satellite radar observations and an original state-of-the-art interpretation technique. Here we show the detection of the onset of volcanic unrest on La Palma island, most likely decades before a potential eruption. We study its current evolution seeing the spatial and temporal changing nature of activity at this potentially dangerous volcano at unprecedented spatial resolutions and long time scales, providing insights into the dynamic nature of the associated volcanic hazard. The geodetic techniques employed here allow tracking of the fluid migration induced by magma injection at depth and identifying the existence of dislocation sources below Cumbre Vieja volcano which could be associated with a future flank failure. Therefore they should continue being monitored using these and other techniques. The results have implications for the monitoring of steep-sided volcanoes at oceanic islands.
Psychosocial morbidity profile in a community based sample of low back pain patients
Authors: Asrar Mir Mahmood , Ghai Babita , Pushpendra Dhanuk , Bansal Dipika 
Published: January 2021

AbstractLow back pain (LBP) is a major health concern and is closely associated with psychosocial morbidity and diminished Health-related quality of life (HRQoL). This is minimally investigated in community-based samples of developing nations like India. This study is aimed to specifically investigate the exposure-outcome associations between LBP and burden of disability (Modified Oswestry questionnaire (MODQ)), psychological morbidities(Depression, Anxiety and Stress Scale (DASS-21)),and HRQoL(Short Form -12 version 2 (SF12V2).A Cross-sectional study using a community-based sample of LBP positive population was conducted. The range of treatment options sought was also collected. Chi-square tests and independent t-test were used to analyze the data. Of 1531 recruited participants, 871(57%) were identified as LBP positive of whom 60% were females. Mean (SD) of age and pain intensity of LBP patients was 33 (11) years and numeric rating scale4.2 (2.6) respectively. Two-third reported minimal/moderate disability. Mean (SD) scores of depression 11.87 (4.05), anxiety (8.32), stress 13.7 (5.98), physical and mental summary scores of SF-12v2 were 47.9 (7.4) and 42.2 (10.4). A multitude of remedial options was sought for the ailment. LBP causes significant disability and psychological morbidity among affected population. This may adversely affect their HRQoL and subsequently productivity. Acupuncture was a preferred treatment sought by Indian LBP patients.
Mapping the immune environment in clear cell renal carcinoma by single-cell genomics
Authors: Borcherding Nicholas , Vishwakarma Ajaykumar , Voigt Andrew P. , Bellizzi Andrew , Kaplan Jacob , Nepple Kenneth , Salem Aliasger K. , Jenkins Russell W. , Zakharia Yousef , Zhang Weizhou 
Published: January 2021

AbstractClear cell renal cell carcinoma (ccRCC) is one of the most immunologically distinct tumor types due to high response rate to immunotherapies, despite low tumor mutational burden. To characterize the tumor immune microenvironment of ccRCC, we applied single-cell-RNA sequencing (SCRS) along with T-cell-receptor (TCR) sequencing to map the transcriptomic heterogeneity of 25,688 individual CD45+ lymphoid and myeloid cells in matched tumor and blood from three patients with ccRCC. We also included 11,367 immune cells from four other individuals derived from the kidney and peripheral blood to facilitate the identification and assessment of ccRCC-specific differences. There is an overall increase in CD8+ T-cell and macrophage populations in tumor-infiltrated immune cells compared to normal renal tissue. We further demonstrate the divergent cell transcriptional states for tumor-infiltrating CD8+ T cells and identify a MKI67 + proliferative subpopulation being a potential culprit for the progression of ccRCC. Using the SCRS gene expression, we found preferential prediction of clinical outcomes and pathological diseases by subcluster assignment. With further characterization and functional validation, our findings may reveal certain subpopulations of immune cells amenable to therapeutic intervention.
Elucidating the tunability of binding behavior for the MERS-CoV macro domain with NAD metabolites
Authors: Lin Meng-Hsuan , Cho Chao-Cheng , Chiu Yi-Chih , Chien Chia-Yu , Huang Yi-Ping , Chang Chi-Fon , Hsu Chun-Hua 
Published: January 2021

AbstractThe macro domain is an ADP-ribose (ADPR) binding module, which is considered to act as a sensor to recognize nicotinamide adenine dinucleotide (NAD) metabolites, including poly ADPR (PAR) and other small molecules. The recognition of macro domains with various ligands is important for a variety of biological functions involved in NAD metabolism, including DNA repair, chromatin remodeling, maintenance of genomic stability, and response to viral infection. Nevertheless, how the macro domain binds to moieties with such structural obstacles using a simple cleft remains a puzzle. We systematically investigated the Middle East respiratory syndrome-coronavirus (MERS-CoV) macro domain for its ligand selectivity and binding properties by structural and biophysical approaches. Of interest, NAD, which is considered not to interact with macro domains, was co-crystallized with the MERS-CoV macro domain. Further studies at physiological temperature revealed that NAD has similar binding ability with ADPR because of the accommodation of the thermal-tunable binding pocket. This study provides the biochemical and structural bases of the detailed ligand-binding mode of the MERS-CoV macro domain. In addition, our observation of enhanced binding affinity of the MERS-CoV macro domain to NAD at physiological temperature highlights the need for further study to reveal the biological functions.
Atomic insight into spin, charge and lattice modulations at SrFeO3−x/SrTiO3 interfaces
Authors: Xu Kun , Gu Youdi , Song Cheng , Zhong Xiaoyan , Zhu Jing 
Published: January 2021

Interfacial lattice, charge and spin order parameters at atomic scale are investigated in SrFeO3−x epitaxial films.
A Questionnaire Survey About Support Requests From School-Age Children and Adolescents Who Stutter
Authors: Iimura Daichi , Ishida Osamu , Takahashi Saburo , Yokoi Hideaki , Miyamoto Shoko 
Published: January 2021

PurposeChildren who stutter (CWS) face communication difficulties in school activities and at home. Although the importance of receiving support from their surroundings has been documented, few studies have investigated potential requests of CWS from their surroundings. This study aimed to elucidate such requests.MethodA total of 43 school-age children and 25 adolescents who stutter completed a free-description questionnaire, including questions such as “what you want your classmates/your classroom teacher/your family to do about your stuttering?” Their descriptions were summarized and categorized based on similarity.ResultsThe results indicate that 90.6% of the participants had more than one request for their classmates, classroom teacher, or family. A total of 197 items were extracted and categorized into seven themes. In particular, the responses included “listen attentively,” “treat us naturally,” and “make arrangements.” While participants tended to hope for classmates or family to “listen attentively” and “treat us naturally,” the request to “make arrangements” was higher for their teacher. Their potential requests varied by age: While school-age CWS wanted people around them to “listen carefully,” the hope of adolescents who stutter was “treat us naturally.”ConclusionsThe various potential requests of CWS were categorized, and the responses shed light on the importance of increasing knowledge of stuttering. The difference between the requests could reflect psychosocial differences between school-age children and adolescents who stutter. In addition, social interaction among peers is more developed in adolescents, and they could harbor fear of being excluded within their community.
Magnetic field line random walk and solar energetic particle path lengths
Authors: Chhiber R. , Matthaeus W. H. , Cohen C. M. S. , Ruffolo D. , Sonsrettee W. , Tooprakai P. , Seripienlert A. , Chuychai P. , Usmanov A. V. , Goldstein M. L. , McComas D. J. , Leske R. A. , Szalay J. R. , Joyce C. J. , Cummings A. C. , Roelof E. C. , Christian E. R. , Mewaldt R. A. , Labrador A. W. , Giacalone J. , Schwadron N. A. , Mitchell D. G. , Hill M. E. , Wiedenbeck M. E. , McNutt R. L. , Desai M. I. 
Published: January 2021

Context. In 2020 May-June, six solar energetic ion events were observed by the Parker Solar Probe/IS⊙IS instrument suite at ≈0.35 AU from the Sun. From standard velocity-dispersion analysis, the apparent ion path length is ≈0.625 AU at the onset of each event.
Aims. We develop a formalism for estimating the path length of random-walking magnetic field lines to explain why the apparent ion path length at an event onset greatly exceeds the radial distance from the Sun for these events.
Methods. We developed analytical estimates of the average increase in path length of random-walking magnetic field lines, relative to the unperturbed mean field. Monte Carlo simulations of field line and particle trajectories in a model of solar wind turbulence were used to validate the formalism and study the path lengths of particle guiding-center and full-orbital trajectories. The formalism was implemented in a global solar wind model, and the results are compared with ion path lengths inferred from IS⊙IS observations.
Results. Both a simple estimate and a rigorous theoretical formulation are obtained for field-lines’ path length increase as a function of path length along the large-scale field. From simulated field line and particle trajectories, we find that particle guiding centers can have path lengths somewhat shorter than the average field line path length, while particle orbits can have substantially longer path lengths due to their gyromotion with a nonzero effective pitch angle.
Conclusions. The long apparent path length during these solar energetic ion events can be explained by (1) a magnetic field line path length increase due to the field line random walk and (2) particle transport about the guiding center with a nonzero effective pitch angle due to pitch angle scattering. Our formalism for computing the magnetic field line path length, accounting for turbulent fluctuations, may be useful for application to solar particle transport in general.
Heart Rate-based Lactate Minimum Test in Running and Cycling
Authors: Perret Claudio , Hartmann Kathrin 
Published: January 2021

AbstractThe heart rate-based lactate minimum test is a highly reproducible exercise test. However, the relation between lactate minimum determined by this test and maximal lactate steady state in running and cycling is still unclear. Twelve endurance-trained men performed this test in running and cycling. Exercise intensity at maximal lactate steady state was determined by performing several constant heart rate endurance tests for both exercise modes. Heart rate, power output, lactate concentration, oxygen uptake and rating of perceived exertion at lactate minimum, maximal lactate steady state and maximal performance were analysed. All parameters were significantly higher at maximal lactate steady state compared to lactate minimum for running and cycling. Significant correlations (p<0.05) between maximal lactate steady state and lactate minimum data were found. Peak heart rate and peak oxygen uptake were significantly higher for running versus cycling. Nevertheless, the exercise mode had no influence on relative (in percentage of maximal values) heart rate at lactate minimum (p=0.099) in contrast to relative power output (p=0.002). In conclusion, all measured parameters at lactate minimum were significantly lower but highly correlated with values at maximal lactate steady state in running and cycling, which allows to roughly estimate exercise intensity at maximal lactate steady state with one single exercise test.
Visible-Light-Mediated Z-Stereoselective Monoalkylation of β,β-Dichlorostyrenes by Photoredox/Nickel Dual Catalysis
Authors: Ollivier Cyril , Fensterbank Louis , Abdellaoui Mehdi , Millanvois Alexandre , Levernier Etienne 
Published: January 2021

AbstractMetal-catalyzed alkylation of 1,1-dihalovinyl moiety commonly suffers from both a lack of stereoselectivity and the overreaction leading to the dialkylation product. The methodology described herein features a new pathway to alkylate stereoselectively β,β-dichlorostyryl substrates to provide the Z-trisubstituted olefin only with fair to good yields. This cross-coupling reaction bears on the smooth and photoinduced formation of a C-centered radical that engages in a nickel-catalyzed organometallic cycle to form the key Csp2–Csp3 bond.
Iridium-Catalyzed Direct Amidation of Imidazoles at the C-2 Position with Isocyanates in the Presence of Hydrosilanes Leading to Imidazole-2-Carboxamides
Authors: Fukumoto Yoshiya , Shiratani Motohiro , Noguchi Hikaru , Chatani Naoto 
Published: January 2021

AbstractRegioselective coupling reaction of N-substituted imidazoles with isocyanates in the presence of a stoichiometric amount of hydrosilanes catalyzed by Ir4(CO)12 to give imidazole-2-carboxamides is reported. Imidazoles bearing an (O-silyl)carboximidate group at the 2-position appear to be initially formed in the reaction; these are then hydrolyzed to the final products in situ. The addition of the hydrosilane was essential for the catalytic reaction to proceed. Substituents on the imidazole ring had no effect on the reaction, except for certain bulky substituents such as
t
Bu and Ph groups at the 4-position. Triazoles such as 4-methyl-4H-1,2,4-triazole and 1-methyl-1H-1,2,4-triazole were also applicable to this C–H amidation, and the latter reaction proceeded regioselectively at the carbon atom between the sp3 and sp2 nitrogen atoms of the ring, and not between the two sp2 nitrogen atoms.
Dutch Translation and Validation of the FACE-Q Rhinoplasty Module
Authors: Pingnet Laura , Verkest Valérie , Fransen Erik , Declau Frank 
Published: January 2021

AbstractFACE-Q was developed by Klassen et al in 2010 as a validated psychometric evaluation instrument for patients undergoing aesthetic surgery. The aim of this study was to translate, adapt, and validate the FACE-Q rhinoplasty module into a Dutch version of the FACE-Q questionnaire conceptually equivalent to the original English version. “Satisfaction with nose” and “satisfaction with nostrils” questionnaires were used and translated from English into Dutch. The translation process and cross-cultural adaptation were conducted in accordance to the International Society for Pharmacoeconomics and Outcomes Research and World Health Organization guidelines. Psychometric validation was performed prospectively on a patient cohort of 30 patients. Each step in the translation process allowed us to make changes to achieve a conceptual translation equivalent to the original version. Psychometric validation revealed highly significant values for internal consistency, test–retest reliability, and responsiveness. The use of international translation guidelines, with a strict translation–back-translation process, led to a Dutch version of the FACE-Q rhinoplasty module. Statistical validation proved the conceptual correspondence with the original English version. The FACE-Q rhinoplasty module is an adequate instrument for determining successful aesthetic surgery based on patient satisfaction. This tool measures twofold: the degree of success with respect to the patient as well as being an assessment tool for the surgeon. We hope this will provide an additional tool to the clinician evaluating the Dutch-speaking rhinoplasty patient.
Breast Cancer Management during Coronavirus Disease 2019 Pandemic: A Literature Review
Authors: Kurniawan Andree , Halim Devina Adella , Giselvania Angela , Sutandyo Noorwati , Panigoro Sonar Soni , Adisasmita Asri , Hatma Ratna Djuwita 
Published: January 2021

Introduction Breast cancer is the most prevalent cancer found in women. The World Health Organization declared the coronavirus disease 2019 (COVID-19) as a public health emergency of international concern. Delaying treatment was associated with mortality. The aim is to evaluate breast cancer management during the pandemic.
Methods We searched articles evaluating the management of breast cancer in the midst of COVID-19 outbreak, by searching in PubMed, PubMed Central, and Oncology Society related websites. We only included articles discussing adult breast cancer management in COVID-19 era. The two reviewers screened the titles and abstracts.
Results Around 124 articles were found through the searching process. Eight observational trials, two review articles, six guidelines or recommendations, and one letter to the editor were included in final review. It was concluded that breast cancer posed a risk to contract COVID-19. Newly suspected breast cancer could be deferred for screening or diagnosis, except for special breast cancer cases with bleeding, invasive, and complicated tumors. The benefit of radiotherapy option in every case should be carefully weighed against the risk of infection. Oral chemotherapy and hormonal therapy drugs were preferred to be given than intravenous chemotherapy. The scheduled chemotherapy could be adjusted to reduce hospital visit. Each breast cancer patient is different, so they should be discussed in multidisciplinary team consisting of surgical, radiation, and medical oncologists, pathologist, radiologist, palliative care people, nurses, nutritionist, and others related to the field.
Conclusion The International Oncology Society has released recommendations for breast cancer during COVID-19 pandemic but there is still questionable-quality evidence.
Bilateral Advancement Heart-Shaped Anoplasty for Severe Anal Stenosis: A New technique
Authors: Khanna Ajay K. , Khanna Soumya 
Published: January 2021

AbstractSevere anal stenosis is an unusual complication following hemorrhoidectomy but leads to severe morbidity. The treatment of severe anal stenosis is usually some type of anoplasty. We designed a heart-shaped bilateral advancement anoplasty for the correction of this condition. We carried out this operation in five patients with successful results.