bluuebunny/crossref_metadata_2025_split huggingface dataset:
HIV trends and disparities by gender and urban–rural residence among adolescents in sub-Saharan Africa
Authors: Maulide Cane Réka , Melesse Dessalegn Y. , Kayeyi Nkomba , Manu Adom , Wado Yohannes Dibaba , Barros Aluisio , Boerma Ties 
Published: June 2021

Abstract
Background
In sub-Saharan Africa HIV transmission is a major challenge in adolescents, especially among girls and those living in urban settings. Major international efforts have aimed at reducing sexual transmission of HIV. This analysis aims to assess the trends in HIV prevalence by gender in adolescents, as well as urban–rural disparities.

Methods
HIV prevalence data at ages 15–19 years were obtained for 31 countries with a national survey since 2010 and for 23 countries with one survey circa 2005 and a recent survey circa 2015. Country medians and average annual rates of changes were used to summarize the trends for two subregions in sub-Saharan Africa, Eastern and Southern Africa and West and Central Africa, which largely correspond with higher and lower HIV prevalence countries. Data on HIV incidence at ages 15–24 and prevalence at 5–9 and 10–14 years were reviewed from 11 recent national surveys. Trends in urban–rural disparities in HIV prevalence and selected indicators of sexual and HIV testing behaviours were assessed for females and males 15–24 years, using the same surveys.

Results
HIV prevalence among girls 15–19 years declined in eastern and Southern Africa from 5.7 to 2.6% during 2005–2015 (country median), corresponding with an average annual rate of reduction of 6.5% per year. Among boys, the median HIV prevalence declined from 2.1 to 1.2%. Changes were also observed in West and Central Africa where median HIV prevalence among girls decreased from 0.7 to 0.4% (average annual rate of reduction 5.9%), but not for boys (0.3%). Girl-boy differences at 10–14 years were small with a country median HIV of 1.0% and 1.3%, respectively. Urban females and males 15–24 had at least 1.5 times higher HIV prevalence than their rural counterparts in both subregions, and since the urban–rural declines were similar, the gaps persisted during 2005–2015.

Conclusions
HIV prevalence among adolescents declined in almost all countries during the last decade, in both urban and rural settings. The urban–rural gap persisted and HIV transmission to girls, but not boys, is still a major challenge in Eastern and Southern African countries.
Developing a provincial patient support network for children and families affected by Tourette syndrome and/or obsessive–compulsive disorder: results of a stakeholder consultation
Authors: Fletcher Julian , Dimitropoulos Gina , Martino Davide , Wilcox Gabrielle , MacMaster Frank , Arnold Paul , Pringsheim Tamara 
Published: June 2021

Abstract
Background
Tourette syndrome and OCD are disorders that frequently occur in children and cause a high level of disability. In Alberta there is a huge delivery gap in providing healthcare services for children with TS and OCD. A stakeholder consultation was performed to ascertain how service delivery could be improved across the province and to inform the development of a provincial information and support organization, the Tourette OCD Alberta Network.

Methods
A mixed-methods study was employed: 10 parents were recruited for interview and 140 parents responded to a survey.

Results
Qualitative data showed there was often an absence of a clear pathway to access healthcare for people with TS and OCD. The negative impact of not receiving treatment, information, and resources in a timely and prompt manner was also revealed. Good clinical practice existed across the province but too often it was hindered by a shortage of knowledge about TS and OCD. In schools, learning for students with TS and OCD was also impaired by educators’ lack of knowledge and preparedness in relation to the disorders.

Conclusions
This study identified ways that challenges with healthcare access, school learning, and seeking information can be overcome. Skills-based training webinars, educational outreach in schools, and peer support were recognized as actions for improving healthcare outcomes for people with TS and OCD. The aim of the Tourette OCD Alberta Network is to provide services and support that directly address the healthcare service delivery shortfalls shown in this study.
Adolescents’ experiences of the information they received about the coronavirus (Covid-19) in Norway: a cross-sectional study
Authors: Kaiser Sabine , Kyrrestad Henriette , Martinussen Monica 
Published: June 2021

Abstract
Background
In the first months of the coronavirus (Covid-19) pandemic, many countries took radical prevention measures. Authorities had to communicate with the public regularly to explain and ensure compliance with these measures and promote safety. The information given by authorities was mainly developed for adults, but children and adolescents may have different needs when it comes to information. This study examined how adolescents perceived information about Covid-19 provided by the media and other sources, and about what topics adolescents reported they lacked information during the first months of the Covid-19 pandemic.

Methods
Three hundred seventy-seven adolescents in 11th, 12th, and 13th grade in Norwegian upper secondary schools (67% girls) completed an online questionnaire. Analyses included descriptive statistics, in addition to Generalized Linear Mixed Models analyses to examine gender differences in adolescents’ satisfaction with the information provided about Covid-19, to what extent the pandemic affected their everyday life, and to what extent they were concerned about becoming infected with Covid-19.

Results
The results showed that the majority of adolescents used the internet as the main source of information about Covid-19, followed by (online) newspapers. About half (49%) reported that they were satisfied with the information available, while 39% were neither satisfied nor dissatisfied, and 12% were dissatisfied. Adolescents wanted more information about the school situation, as well as virus- and future-related topics. A total of 21% reported that they were concerned about becoming infected with Covid-19. There was no significant gender difference in overall satisfaction with the information provided about Covid-19. Girls reported being significantly more affected by the pandemic than boys, and that they were significantly more concerned than boys about becoming infected with Covid-19.

Conclusions
This survey provides important knowledge to professionals working with adolescents, as well as to authorities, about what information channels can be used to reach adolescents, and what information they lacked about the Covid-19 pandemic.
Novel SCNN1A gene splicing-site mutation causing autosomal recessive pseudohypoaldosteronism type 1 (PHA1) in two Italian patients belonging to the same small town
Authors: Serra Gregorio , Antona Vincenzo , D’Alessandro Maria Michela , Maggio Maria Cristina , Verde Vincenzo , Corsello Giovanni 
Published: June 2021

Abstract
Introduction
Pseudohypoaldosteronism type 1 (PHA1) is a rare genetic disease due to the peripheral resistance to aldosterone. Its clinical spectrum includes neonatal salt loss syndrome with hyponatremia and hypochloraemia, hyperkalemia, metabolic acidosis and increased plasmatic levels of aldosterone. Two genetically distinct forms of disease, renal and systemic, have been described, showing a wide clinical expressivity. Mutations in the genes encoding for the subunits of the epithelial sodium channels (ENaC) are responsible for generalized PHA1.

Patients’ presentation
We hereby report on two Italian patients with generalized PHA1, coming from the same small town in the center of Sicily. The first patient is a male child, born from the first pregnancy of healthy consanguineous Sicilian parents. A novel SCNN1A (sodium channel epithelial subunit alpha) gene mutation, inherited from both heterozygous parents, was identified by next generation sequencing (NGS) in the homozygous child (and later, also in the heterozygous maternal aunt). A more detailed family history disclosed a possible related twenty-year-old girl, belonging to the same Sicilian small town, with referred neonatal salt loss syndrome associated to hyperkalemia, and subsequent normal growth and neurodevelopment. This second patient had a PHA1 clinical diagnosis when she was about 1 year old. The genetic investigation was, then, extended to her and to her family, revealing the same mutation in the homozygous girl and in the heterozygous parents.

Conclusions
The neonatologist should consider PHA1 diagnosis in newborns showing hyponatremia, hyperkalemia and metabolic acidosis, after the exclusion of a salting-loss form of adrenogenital syndrome. The increased plasmatic levels of aldosterone and aldosterone/renin ratio, associated to a poor response to steroid administration, confirmed the diagnosis in the first present patient. An accurate family history may be decisive to identify the clinical picture. A multidisciplinary approach and close follow-up evaluations are requested, in view of optimal management, adequate growth and development of patients. Next generation sequencing (NGS) techniques allowed the identification of the SCNN1A gene mutation either in both patients or in other heterozygous family members, enabling also primary prevention of disease. Our report may broaden the knowledge of the genetic and molecular bases of PHA1, improving its clinical characterization and providing useful indications for the treatment of patients. Clinical approach must be personalized, also in relation to long-term survival and potential multiorgan complications.
Genetic diversity of vector-borne pathogens in spotted and brown hyenas from Namibia and Tanzania relates to ecological conditions rather than host taxonomy
Authors: Krücken Jürgen , Czirják Gábor Á. , Ramünke Sabrina , Serocki Maria , Heinrich Sonja K. , Melzheimer Jörg , Costa M. Carolina , Hofer Heribert , Aschenborn Ortwin H. K. , Barker Nancy A. , Capodanno Stefano , de Carvalho Luís Madeira , von Samson-Himmelstjerna Georg , East Marion L. , Wachter Bettina 
Published: June 2021

Abstract
Background
Improved knowledge on vector-borne pathogens in wildlife will help determine their effect on host species at the population and individual level and whether these are affected by anthropogenic factors such as global climate change and landscape changes. Here, samples from brown hyenas (Parahyaena brunnea) from Namibia (BHNA) and spotted hyenas (Crocuta crocuta) from Namibia (SHNA) and Tanzania (SHTZ) were screened for vector-borne pathogens to assess the frequency and genetic diversity of pathogens and the effect of ecological conditions and host taxonomy on this diversity.

Methods
Tissue samples from BHNA (n = 17), SHNA (n = 19) and SHTZ (n = 25) were analysed by PCRs targeting Anaplasmataceae, Rickettsia spp., piroplasms, specifically Babesia lengau-like piroplasms, Hepatozoidae and filarioids. After sequencing, maximum-likelihood phylogenetic analyses were conducted.

Results
The relative frequency of Anaplasmataceae was significantly higher in BHNA (82.4%) and SHNA (100.0%) than in SHTZ (32.0%). Only Anaplasma phagocytophilum/platys-like and Anaplasma bovis-like sequences were detected. Rickettsia raoultii was found in one BHNA and three SHTZ. This is the first report of R. raoultii from sub-Saharan Africa. Babesia lengau-like piroplasms were found in 70.6% of BHNA, 88.9% of SHNA and 32.0% of SHTZ, showing higher sequence diversity than B. lengau from South African cheetahs (Acinonyx jubatus). In one SHTZ, a Babesia vogeli-like sequence was identified. Hepatozoon felis-like parasites were identified in 64.7% of BHNA, 36.8% of SHNA and 44.0% of SHTZ. Phylogenetic analysis placed the sequences outside the major H. felis cluster originating from wild and domestic felids. Filarioids were detected in 47.1% of BHNA, 47.4% of SHNA and 36.0% of SHTZ. Phylogenetic analysis revealed high genetic diversity and suggested the presence of several undescribed species. Co-infections were frequently detected in SHNA and BHNA (BHNA median 3 pathogens, range 1–4; SHNA median 3 pathogens, range 2–4) and significantly rarer in SHTZ (median 1, range 0–4, 9 individuals uninfected).

Conclusions
The frequencies of all pathogens groups were high, and except for Rickettsia, multiple species and genotypes were identified for each pathogen group. Ecological conditions explained pathogen identity and diversity better than host taxonomy.

Graphical Abstract
Identification of monoclonal antibodies against human renal glomerular endothelial cells in lupus nephritis that induce endothelial interferon-alpha production
Authors: Hu Ya-Chiao , Tsai I-Jung , Hsu Hui-Yao , Chiang Bor-Luen , Yang Yao-Hsu 
Published: June 2021

Abstract
Background
The pathogenesis of lupus nephritis (LN) remains not fully understood. In this study, we aimed to explore the pathogenic roles of autoantibodies against human renal glomerular endothelial cells (HRGEC) in LN patients.

Methods
The serum levels of anti-HRGEC antibodies in systemic lupus erythematosus (SLE) patients without LN and LN patients were determined by cell-based enzyme-linked immunosorbent assay (ELISA). Monoclonal IgG anti-HRGEC antibodies were subsequently generated from LN patients. The binding activities of these monoclonal antibodies to HRGEC, their cross-reactivity with double-stranded DNA (dsDNA), and the ability to activate HRGEC were further evaluated.

Results
LN patients had higher serum levels of IgG anti-HRGEC antibodies than SLE patients without LN and healthy controls. Four monoclonal IgG anti-HRGEC antibodies (LN1–4) were obtained; LN1 and LN2 were IgG3 while LN3 and LN4 were IgG1. Among these monoclonal antibodies, LN1–3 were cross-reactive with dsDNA. The functional assays showed that compared with IgG1/IgG3 isotype controls, LN3 had an effect on HRGEC to enhance interleukin (IL)-6 production, LN4 could enhance IL-8 and monocyte chemoattractant protein (MCP)-1 production, and LN1–3 possessed the ability to induce interferon (IFN)-α production by HRGEC. Moreover, the removal of DNA on the HRGEC surface by DNAse 1 did not interpose the binding of LN1–3 to HRGEC and the effects of LN1–3 on IFN-α induction by HRGEC.

Conclusions
Some IgG anti-HRGEC antibodies in LN patients had the ability to enhance endothelial proinflammatory cytokine (IL-6, IL-8, and MCP-1) production, and some could induce the DNA-independent production of IFN-α by HRGEC.
Protection of coenzyme Q10 against contrast-induced acute kidney injury in male diabetic rats
Authors: Couto Sheila Marques Fernandes , da Fonseca Cassiane Dezoti , Watanabe Mirian , de Fátima Fernandes Vattimo Maria 
Published: June 2021

Abstract
Background
Diabetes mellitus (DM) is a major risk factor for contrast-induced acute kidney injury (CI-AKI). DM and CI-AKI result in oxidative damage and inflammation that can be reduced when treated with the coenzyme Q-10 (CoQ10). The aim of this study was to investigate the therapeutic potential of CoQ10 in renal function, renal hemodynamics, oxidative profile and renal histology in diabetic rats subjected to CI-AKI.

Methods
Wistar rats, male, randomized into five groups: citrate: control animals received citrate buffer (streptozotocin vehicle, 0.4 mL); Tween: control animals of CoQ10 treatment received 1% Tween 80 (CoQ10 vehicle, 0.5 mL); DM: animals that received streptozotocin (60 mg/kg); DM + IC: DM animals treated with iodinated contrast (IC, 6 mL/kg); DM + IC + CoQ10: DM animals treated with CoQ10 (10 mg/kg) and that received IC (6 mL/kg). The protocols lasted 4 weeks. An evaluation was made to measure renal function, inulin clearance and serum creatinine, renal hemodynamics by renal blood flow (RBF) and renal vascular resistance (RVR), markers of oxidative stress such as urinary peroxides and nitrate, lipid peroxidation, thiols in renal tissue and renal histological analysis.

Results
DM animals showed reduced renal function, which was followed by an increase inserum creatinine and significant reduction of inulin clearance and RBF. It was noticed an increase in RVR and redox imbalance with higher urinary peroxides and nitrate lipid peroxidation levels with depletion of thiols in renal tissue. IC treatment exacerbated these changes in DM + IC. CoQ10 administration ameliorated renal function, prevented hemodynamic changes and neutralized oxidative damage and progression of the histologic damage in the DM + IC + CoQ10 group.

Conclusion
This study demonstrated the renoprotection properties of CoQ10 in an experimental model of risk factor of DM for CI-AKI. CoQ10 presented an antioxidant effect on the CI-AKI in male diabetic rats by improving renal function and renal hemodynamics, preserving morphology and reducing oxidative stress.
Artificial intelligence in medical imaging practice in Africa: a qualitative content analysis study of radiographers’ perspectives
Authors: Antwi William Kwadwo , Akudjedu Theophilus N. , Botwe Benard Ohene 
Published: June 2021

AbstractPurposeStudies have documented the clinical potentials of artificial intelligence (AI) in medical imaging practice to improving patient care. This study aimed to qualitatively explore the perception of radiographers relating to the integration of AI in medical imaging practice in Africa.MethodsThe study employed a qualitative design using an open-ended online instrument administered between March and August 2020. Participants consisted of radiographers working within Africa during the time of the study. Data obtained were analysed using qualitative content analysis. Six themes of concerns were generated: expectant tool; career insecurity; cost of new technology, equipment preservation and data insecurity; service delivery quality; need for expanding AI awareness.ResultsA total of 475 valid responses were obtained. Participants demonstrated a positive outlook about AI in relation to clinical quality improvement, competent diagnosis, radiation dose reduction and improvement in research. They however expressed concerns relating to the implementation of this technology, including job security and loss of core professional radiographer skills and roles. In addition, concerns regarding AI equipment maintenance, lack of awareness about AI and education and training opportunities were evident.ConclusionAwareness of the importance of AI in medical imaging practice was acknowledged; however, concerns relating to job security, data protection must be given critical attention for successful implementation of these advanced technologies in medical imaging in Africa. Inclusion of AI modules in the training of future radiographers is highly recommended.
Postoperative wound assessment in cattle: How reliable is the back hand palpation?
Authors: Proios Ioannis , Kusenda Marian , Seiler Christian , Siewert Carsten , Seifert Hermann , Kaske Martin 
Published: June 2021

AbstractBackgroundAs part of clinical wound assessment in bovine surgery, discrepancies in skin temperature are evaluated by placing the back of the hand on the area to be examined. Generally, an increased skin temperature at the wound site for a prolonged period is considered as an indicator of impaired wound healing.The aim of this study was to verify the reliability of palpation under bovine practice conditions using laparotomy as an example. Fourteen cows (German Holstein) with a left displacement of the abomasum (LDA) without other severe concurrent diseases were examined preoperatively and once daily for ten days after surgery. The skin temperature of the wound site in the right flank was assessed by palpation, followed by thermographic evaluation using an infrared camera after a 45-min acclimatisation period, under standardised conditions in a closed examination room daily for 10 days.ResultsAll the incisions healed without clinical detectable perturbances. The ambient temperature range during the study period was 7.8 − 24.1 °C. Two groups were retrospectively defined according to the ambient temperature: high ambient temperature (HT group; median: 20.2 °C 25/75 quartile: 18.5 °C / 21.7 °C;n = 6) and low ambient temperature (LT group; 10.8 °C; 9.4 °C / 12.8 °C;n = 8). The temperature differences (Δϑ) between the mean skin temperature of the wound site and a defined reference area cranial to the wound were assessed. A significant negative correlation was found between the ambient temperature (ϑAmb) and Δϑ (r=-0.51;P < 0.001). The Δϑ was postoperatively higher in the cows in the LT group (median of the individual animals 0.8–2.5 °C) than in the HT group (0.1–0.5 °C;P < 0.05). In contrast to the thermographic findings, manual palpation rarely detected local hyperthermia (> 1 °C) at the wound site (sensitivity 0.20; specificity 0.96).ConclusionsThe infrared thermography provides a more reliable assessment of temperature changes at the wound site in comparison to manual palpation. The ambient temperature markedly affects the extent of local hyperthermia at the wound site.
Feature compensation based on independent noise estimation for robust speech recognition
Authors: Lü Yong , Lin Han , Wu Pingping , Chen Yitao 
Published: June 2021

AbstractIn this paper, we propose a novel feature compensation algorithm based on independent noise estimation, which employs a Gaussian mixture model (GMM) with fewer Gaussian components to rapidly estimate the noise parameters from the noisy speech and monitor the noise variation. The estimated noise model is combined with a GMM with sufficient Gaussian mixtures to produce the noisy GMM for the clean speech estimation so that parameters are updated if and only if the noise variation occurs. Experimental results show that the proposed algorithm can achieve the recognition accuracy similar to that of the traditional GMM-based feature compensation, but significantly reduces the computational cost, and thereby is more useful for resource-limited mobile devices.
Starch extracted from pineapple (Ananas comosus) plant stem as a source for amino acids production
Authors: Chu Pei Hsia , Jenol Mohd Azwan , Phang Lai Yee , Ibrahim Mohamad Faizal , Prasongsuk Sehanat , Bankeeree Wichanee , Punnapayak Hunsa , Lotrakul Pongtharin , Abd-Aziz Suraini 
Published: June 2021

Abstract
Background
Pineapple plant (Ananas comosus) is one of the largest productions in Asia and its increasing production has generated a huge amount of pineapple wastes. Pineapple plant stem is made up of high concentration of starch which can potentially be converted into value-added products, including amino acids. Due to the increasing demand in animal feed grade amino acids, especially for methionine and lysine, the utilisation of cheap and renewable source is deemed to be an essential approach. This study aimed to produce amino acids from pineapple plant stem hydrolysates through microbial fermentation by Pediococcus acidilactici Kp10. Dextrozyme was used for hydrolysis of starch and Celluclast 1.5 L for saccharification of cellulosic materials in pineapple plant stem.

Results
The hydrolysates obtained were used in the fermentation to produce methionine and lysine. Pineapple plant stem showed high starch content of 77.78%. Lignocellulosic composition of pineapple plant stem consisted of 46.15% hemicellulose, 31.86% cellulose, and 18.60% lignin. Saccharification of alkaline-treated pineapple plant stem gave lower reducing sugars of 13.28 g/L as compared to untreated, where 18.56 g/L reducing sugars obtained. Therefore, the untreated pineapple plant stem was selected for further process. Starch hydrolysis produced 57.57 g/L reducing sugar (100% hydrolysis yield) and saccharification of cellulosic materials produced 24.67 g/L reducing sugars (56.93% hydrolysis yield). The starch-based and cellulosic-based of pineapple plant stem were subjected as carbon source in methionine and lysine production by P. acidilactici Kp10.

Conclusions
In conclusion, higher methionine and lysine production were produced from starch-based hydrolysis (40.25 mg/L and 0.97 g/L, respectively) as compared to cellulosic-based saccharification (37.31 mg/L and 0.84 g/L, respectively) of pineapple plant stem.

Graphical Abstract