bluuebunny/crossref_metadata_2025_split huggingface dataset:
Spodoptera littoralis genome mining brings insights on the dynamic of expansion of gustatory receptors in polyphagous noctuidae
Authors: Meslin Camille , Mainet Pauline , Montagné Nicolas , Robin Stéphanie , Legeai Fabrice , Bretaudeau Anthony , Johnston J. Spencer , Koutroumpa Fotini , Persyn Emma , Monsempès Christelle , François Marie-Christine , Jacquin-Joly Emmanuelle 
Published: December 2021

AbstractThe bitter taste, triggered via gustatory receptors, serves as an important natural defense against the ingestion of poisonous foods in animals, and the diversity of food diet is usually linked to an increase in the number of gustatory receptor genes. This has been especially observed in polyphagous insect species, such as noctuid species from the Spodoptera genus. However, the dynamic and physical mechanisms leading to these gene expansions and the evolutionary pressures behind them remain elusive. Among major drivers of genome dynamics are the transposable elements but, surprisingly, their potential role in insect gustatory receptors expansion has not been considered yet.In this work, we hypothesized that transposable elements and possibly positive selection would be involved in the active dynamic of gustatory receptor evolution in Spodoptera spp. We first sequenced de novo the full 465Mb genome of S. littoralis, and manually annotated all chemosensory genes, including a large repertoire of 373 gustatory receptor genes (including 19 pseudogenes). We also improved the completeness of S. frugiperda and S. litura gustatory receptor repertoires. Then, we annotated transposable elements and revealed that a particular category of class I retrotransposons, the SINE transposons, was significantly enriched in the vicinity of gustatory receptor gene clusters, suggesting a transposon-mediated mechanism for the formation of these clusters. Selection pressure analyses indicated that positive selection within the gustatory receptor gene family is cryptic, only 7 receptors being identified as positively selected.Altogether, our data provide a new good quality Spodoptera genome, pinpoint interesting gustatory receptor candidates for further functional studies and bring valuable genomic information on the mechanisms of gustatory receptor expansions in polyphagous insect species.
Atypus karschiDönitz, 1887 (Araneae: Atypidae): an Asian purse-web spider established in Pennsylvania, USA
Authors: Řezáč Milan , Tessler Steven , Heneberg Petr , Ávila Herrera Ivalú Macarena , Gloríková Nela , Forman Martin , Řezáčová Veronika , Král Jiří 
Published: December 2021

AbstractThe Mygalomorph spiders of the family Atypidae are among the most archaic spiders. The genusAtypusLatreille, 1804 occurs in Eurasia and northern Africa, with a single enigmatic species,Atypus snetsingeriSarno, 1973, restricted to a small area in southeastern Pennsylvania in Eastern USA. This study was undertaken to learn more about genetics of that species, its habitat requirements and natural history. A close relationship to European species could be assumed based onA. snetsingeri’s occurrence on the eastern coast of the USA, however molecular markers (CO1 sequences) confirmed thatA. snetsingeriis identical withAtypus karschiDönitz, 1887 native to East Asia; it is an introduced species. The specific epithetsnetsingeriis therefore relegated to a junior synonym ofA. karschi.The karyotype ofA. karschihas 42 chromosomes in females and 41 in males (X0 sex chromosome system). Chromosomes were metacentric except for one pair, which exhibited submetacentric morphology. In Pennsylvania the above-ground webs are usually vertical and attached to the base of bushes, trees, or walls, although some webs are oriented horizontally near the ground. It was found in a variety of habitats from forests to suburban shrubbery, and over a wide range of soil humidity and physical parameters. Prey include millipedes, snails, woodlice, carabid beetles and earthworms. The number of juveniles in excavated female webs ranged from 70 to 201.Atypus karschiis the first known case of an introduced purse-web spider. It is rarely noticed but well-established within its range in southeastern Pennsylvania.
Modulation of the Drosophila Transcriptome by Developmental Exposure to Alcohol
Authors: Morozova Tatiana V. , Shankar Vijay , MacPherson Rebecca A. , Mackay Trudy F. C. , Anholt Robert R. H. 
Published: December 2021

AbstractPrenatal exposure to ethanol can cause fetal alcohol spectrum disorder (FASD), a prevalent, preventable pediatric disorder. Identifying genetic risk alleles for FASD is challenging since time, dose, and frequency of exposure are often unknown, and manifestations of FASD are diverse and evident long after exposure. Drosophila melanogaster is an excellent model to study the genetic basis of the effects of developmental alcohol exposure since many individuals of the same genotype can be reared under controlled environmental conditions. We used 96 sequenced, wild-derived inbred lines from the Drosophila melanogaster Genetic Reference Panel (DGRP) to profile genome-wide transcript abundances in young adult flies that developed on ethanol-supplemented medium or standard culture medium. We found substantial genetic variation in gene expression in response to ethanol with extensive sexual dimorphism. We constructed sex-specific genetic networks associated with alcohol-dependent modulation of gene expression that include protein-coding genes, Novel Transcribed Regions (NTRs, postulated to encode long non-coding RNAs) and female-specific coordinated regulation of snoRNAs that regulate pseudouridylation of ribosomal RNA. We reared DGRP lines which showed extreme upregulation or downregulation of snoRNA expression during developmental alcohol exposure on standard or ethanol supplemented medium and demonstrated that developmental exposure to ethanol has genotype-specific effects on adult locomotor activity and sleep. There is significant and sex-specific natural genetic variation in the transcriptional response to developmental exposure to ethanol in Drosophila that comprises networks of genes affecting nervous system development and ethanol metabolism as well as networks of regulatory non-coding RNAs.Summary statementWe developed a Drosophila model for Fetal Alcohol Spectrum Disorder and found that developmental exposure to alcohol modulates expression of networks of genes affecting nervous system development and ethanol metabolism as well as networks of regulatory noncoding RNAs.
The luminal AAA+ ATPase torsinA mediates distinct mechanisms of nuclear-cytoplasmic communication by adopting different functional assembly states
Authors: Hur Kwang-Ho , Hennen Jared , Saunders Cosmo A. , Schoenhoefen Amy , Willey Patrick T. , Morris Cassandra M. , Ibrahim Rabab H. , Maisuria Ravi S. , Mueller Joachim D. , Luxton G.W. Gant 
Published: December 2021

ABSTRACTChemical and mechanical nuclear-cytoplasmic communication across the nuclear envelope (NE) is largely mediated by the nuclear pore complex (NPC) and the linker of nucleoskeleton and cytoskeleton (LINC) complex, respectively. While NPC and LINC complex assembly are functionally related, the mechanisms responsible for this relationship remain poorly understood. Here, we investigated how the luminal ATPases associated with various cellular activities (AAA+) protein torsinA promotes NPC and LINC complex assembly using fluorescence fluctuation spectroscopy (FFS), quantitative photobleaching analyses, and functional cellular assays. We report that torsinA controls LINC complex-dependent nuclear-cytoskeletal coupling as a soluble hexameric AAA+ protein and interphase NPC biogenesis as a membrane-associated helical polymer. These findings help resolve the conflicting models of torsinA function that were recently proposed based on in vitro structural studies. Our results will enable future studies of the role of defective nuclear-cytoplasmic communication in DYT1 dystonia and other diseases caused by mutations in torsinA.
The intrinsic spatiotemporal organization of the human brain - A multi-dimensional functional network atlas
Authors: Li Jian , Liu Yijun , Wisnowski Jessica L. , Leahy Richard M. 
Published: December 2021

AbstractThe human brain is a complex, integrative and segregative network that exhibits dynamic fluctuations in activity across space and time. A canonical set of large-scale networks has been historically identified from resting-state fMRI (rs-fMRI), including the default mode, visual, somatomotor, salience, attention, and executive control. However, the methods used in identification of these networks have relied on assumptions that may inadvertently constrain their properties and consequently our understanding of the human connectome. Here we define a brain “network” as a functional component that jointly describes its spatial distribution and temporal dynamics, where neither domain suffers from unrealistic constraints. Using our recently developed BrainSync algorithm and the Nadam-Accelerated SCAlable and Robust (NASCAR) tensor decomposition, we identified twenty-three brain networks using rs-fMRI data from a large group of healthy subjects acquired by the Human Connectome Project. These networks are spatially overlapped, temporally correlated, and highly reproducible across two independent groups and sessions. We show that these networks can be clustered into six distinct functional categories and naturally form a representative functional network atlas for a healthy population. Using this atlas, we demonstrate that individuals with attention-deficit/hyperactivity disorder display disproportionate brain activity increases, relative to neurotypical subjects, in visual, auditory, and somatomotor networks concurrent with decreases in the default mode and higher-order cognitive networks. Thus, this work not only yields a highly reproducible set of spatiotemporally overlapped functional brain networks, but also provides convergent evidence that individual differences in these networks can be used to explain individual differences in neurocognitive functioning.
Influence of effective polarization on ion and water interactions within a biomimetic nanopore
Authors: Phan Linda X. , Lynch Charlotte I. , Crain Jason , Sansom Mark S.P. , Tucker Stephen J. 
Published: December 2021

AbstractInteractions between ions and water at hydrophobic interfaces within ion channels and nanopores are suggested to play a key role in the movement of ions across biological membranes. Previous molecular dynamics (MD) simulations have shown that the affinity of polarizable anions to aqueous/hydrophobic interfaces can be markedly influenced by including polarization effects through an electronic continuum correction (ECC). Here, we designed a model biomimetic nanopore to imitate the polar pore openings and hydrophobic gating regions found in pentameric ligand-gated ion channels. MD simulations were then performed using both a non-polarizable force field and the ECC method to investigate the behavior of water, Na+ and Cl− ions confined within the hydrophobic region of the nanopore. Number density distributions revealed preferential Cl− adsorption to the hydrophobic pore walls, with this interfacial layer largely devoid of Na+. Free energy profiles for Na+ and Cl− permeating the pore also display an energy barrier reduction associated with the localization of Cl− to this hydrophobic interface, and the hydration number profiles reflect a corresponding reduction in the first hydration shell of Cl−. Crucially, these ion effects were only observed through inclusion of effective polarization which therefore suggests that polarizability may be essential for an accurate description for the behavior of ions and water within hydrophobic nanoscale pores, especially those that conduct Cl−.
Extracellular release of two peptidases dominates generation of the trypanosome quorum-sensing signal
Authors: Tettey Mabel Deladem , Rojas Federico , Matthews Keith R. 
Published: December 2021

SummaryTrypanosomes causing African sleeping sickness use quorum-sensing (QS) to generate transmission-competent stumpy forms in their mammalian hosts. This density-dependent process is signalled by oligopeptides that stimulate the signal transduction pathway leading to stumpy formation. Using mass spectrometry analysis, peptidases released by trypanosomes were identified and, for 12 peptidases, their extracellular delivery was confirmed. Thereafter, the contribution of each peptidase to QS signal production was determined using systematic inducible overexpression in vivo, activity being confirmed to operate through the physiological QS signalling pathway. Gene knockout of the QS-active peptidases identified two enzymes, oligopeptidase B and metallocarboxypeptidase I, that significantly reduced QS when ablated individually. Further, a combinatorial gene knockout of both peptidases confirmed their dominance in the generation of the QS signal, with peptidase release of oligopeptidase B mediated via an unconventional protein secretion pathway. This identifies how the QS signal driving trypanosome virulence and transmission is generated in mammalian hosts.Abstract FigureGraphical Abstract
Lipidomic analysis of adipose-derived extracellular vesicles reveals their potential as lipid mediators of obesity-associated metabolic complications
Authors: Blandin Alexia , Hilairet Grégory , Ponnaiah Maharajah , Ducheix Simon , Dugail Isabelle , Cariou Bertrand , Lhomme Marie , Le Lay Soazig 
Published: December 2021

AbstractAdipose extracellular vesicles (AdEV) transport lipids that could participate to the development of obesity-related metabolic dysfunctions. This study aimed to define mice AdEV lipid signature in either healthy or obesity context by a targeted LC-MS/MS approach.Distinct clustering of AdEV and visceral adipose tissue (VAT) lipidomes by principal component analysis reveals specific lipid composition of AdEV compared to source VAT. Comprehensive analysis identifies enrichment of ceramides and phosphatidylglycerols in AdEV compared to VAT in lean conditions. Lipid subspecies commonly enriched in AdEV highlight specific AdEV-lipid sorting. Obesity impacts AdEV lipidome, driving triacylglycerols and sphingomyelins enrichment in obese versus lean conditions. Obese mice AdEV also display elevated phosphatidylglycerols and acid arachidonic subspecies contents highlighting novel biomarkers and/or mediators of metabolic dysfunctions.Our study identifies specific lipid-fingerprints for plasma, VAT and AdEV that are informative of the metabolic status and underline the signaling capacity of lipids transported by AdEV in obesity-associated complications.
How and when does leader narcissism hinder team radical creativity? The role of team information elaboration and inter-team competition
Authors: Liu Zhiqiang , Zhou Rong , Wei Lihua , Ouyang Xi , Zhou Kong 
Published: December 2021

Purpose
Drawing on social information processing theory and trait activation theory, this study aims to examine the mediating effect of leader narcissism on team radical creativity via team information elaboration and explores the moderating role of inter-team competition.

Design/methodology/approach
Time-lagged and multisource survey data were collected from 86 team leaders and 409 employees in a Chinese company. Path analysis was used to test the hypotheses.

Findings
The results indicated that leader narcissism could impede team radical creativity via team information elaboration. Moreover, the negative indirect effects of leader narcissism on team radical creativity were more pronounced when the inter-team competition was low.

Originality/value
This study makes contributions to the literature on leader narcissism and team radical creativity by examining the detrimental indirect effects of leader narcissism on team radical creativity via team information. Furthermore, it broadens current literature by investigating the potential positive intervention of inter-team competition on the negative aspects of leader narcissism.
From quality control to TQM, service quality and service sciences: a 30-year review of TQM literature
Authors: Chen Chi-Kuang , Reyes Lidia , Dahlgaard Jens , Dahlgaard-Park Su Mi 
Published: December 2021

Purpose
This paper aims to review total quality management (TQM) literature in the past three decades to identify the quality related key terms, to analyse their linkage among the identified key terms and their developmental trends.

Design/methodology/approach
Bibliometric and statistical methods are used to analyse article titles published in the Total Quality Management and Business Excellence journal during 1990–2019. The current research is based on a search from the ProQuest academic database and the journal’s website, resulting in 2,452 articles collected. The VOSviewer and Microsoft Excel were then used for the analyses.

Findings
A total of 52 key terms were extracted from the journal’s 2,452 article titles, the top three key terms in terms of occurrences were “quality,” “total quality management” and “service quality.” Five themes were then proposed from clustering the 52 key terms: “frameworks/models,” “essentials/enablers,” “methods/techniques,” “culture/characteristics” and “effects/results.” Trend analyses were also conducted regarding the five themes in an attempt to highlight the patterns of research publications from 1990 to 2019. It is found that the research publications for “essentials/enablers,” “methods and techniques” and “effects/results” have steadily increased during the analysis period, while “frameworks/models” and “culture/characteristic” have slightly decreased. These insights provide implication for the historical evolution of quality from “quality control,” “total quality management” and “service quality,” combining with the development of “service sciences.”

Originality/value
This paper highlights the concept of quality since its meaning has changed and evolved over time from quality control, TQM to service quality. And it is emerging in the present and future development of service sciences because of both of TQM and service sciences having the same nature of multidisciplinary background and characteristics. Also the authors can conclude that quality and service sciences are in fact two sides of the same coin because both of them having the same duality of “tangible-intangible” and “physical-virtual” faces which are the important topics that TQM should focus on.
Path-dependency theory in a post-conflict state: the case of Fiji
Authors: Loga Patricia , Cardow Andrew , Asquith Andy 
Published: December 2021

Purpose
Violent geo-political conflicts are on the rise across the globe, particularly within fragile states. Using path-dependency theory, this paper aims to explore Fiji in the context of its public administrative history examining the legacies of history that have contributed to its ongoing conflicts.

Design/methodology/approach
An archival document analysis along with a theoretical thematic analysis was used to collect and assess data. Themes were identified that explain how and when the conflict became path-dependent.

Findings
Analysing conflict as path-dependent demonstrates how indirect rule while Fiji was under colonial rule, and the short time it has taken for the nation to transition from a colony to an independent State contributed to the eruption of conflicts in Fiji.

Originality/value
The research makes two key contributions, namely, it develops a theoretical understanding of conflict using path-dependency theory and it uncovers legacies of colonialism that have shaped conflict in Fiji.
Social determinants of mental well-being: senses of powerlessness and empowerment among Romani in Ukraine
Authors: Gorbunova Viktoriia , Gusak Natalia , Klymchuk Vitalii , Palii Valeriia , Ustenko Vitalina , Kondur Zemfira , Popenko Viola , Oates John 
Published: December 2021

Purpose
The purpose of this paper is to explore senses of powerlessness and empowerment among Romani in Ukraine in relation to such social circles as the extended family, the Romani local community and the whole Ukrainian society. The main research interest is focused on situations and factors that make people feel powerless or empowered.

Design/methodology/approach
The study was conducted using a semi-structured interview method via telephone. Romani NGO experts approved the interview content. Trained Roma-facilitators were interviewers. Every interview was transcribed for further thematic analysis.

Findings
The most prominent empowerment factors for Romani people are located within their families and local communities, while the bigger society (Ukrainians themselves and Ukrainian public institutions) is a main source of powerlessness. At the same time, the rigid role of family and community expectations is seen as detrimental for both males and females.

Originality/value
The paper is original in terms of its topic (senses of powerlessness and empowerment among Romani in Ukraine as social determinants of mental well-being) and research strategy (engagement of Roma-facilitators as interviewers).
How the leading Chinese real estate brokerage transformed into a digital platform business
Authors: Zhang Yanli , Yao Lixia 
Published: December 2021

Purpose
In this article, we study how a Chinese real estate broker - Lianjia successfully transformed itself into Beike - China’s leading digital platform for housing transactions and services. We explain the motivation behind this platform transformation, how it turned out, and what are the lessons learned for other companies contemplating a platform transformation. Beike’s lessons are significant as they not only can help the companies achieve growth via platform transformation but also create social value by contributing to higher service quality in traditional service industries.

Design/methodology/approach
We draw upon comprehensive archival research into Beike, and our many years of ongoing research on platform strategy and business growth strategy.

Findings
This article provides important lessons for companies in traditional service industries on how to expand growth via digital platforms. We summarize four key lessons learned: 1) data is central to success in platform transformation; 2) industry knowledge and experience play an important role; 3) the right platform governance is critical in value creation; 4) harness the double powers of platform and digital transformation.

Research limitations/implications
More research on digital platforms and platform transformation in traditional service industries is needed to delve into the underlying factors and delineate the boundary conditions for specific details in this strategy and implementation.

Practical implications
This article is useful to business executives, academics, management consultants, and entrepreneurs interested in learning more about how to use digital platforms to achieve business growth and create economic and social value. In particular, Beike’s case offers inspiration and valuable lessons to companies in traditional service industries and helps them consider the factors that are important in the process of platform transformation.

Social Implications
This article on Beike provides an innovative solution to business leaders in traditional service industries grappling with a lack of professional standards and trust to use digital platforms to elevate service quality and create social value.

Originality/value
This article is unique and add value because Beike is a pioneer of using the digital platform to achieve growth and transform traditional service industries. Our study shows that platform transformation not only can help a company in a traditional industry achieve impressive growth but at the same time can create enormous social value by elevating the service quality of the whole industry.